LOCUS       DATLJT010000001         1582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_32, whole
            genome shotgun sequence.
ACCESSION   DATLJT010000001 DATLJT010000000
VERSION     DATLJT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VM910_00005"
     CDS             complement(<1..968)
                     /locus_tag="VM910_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVI60969.1"
                     /translation="MQRKISRRDFGKRLAGGAALLALQPAWNCLAAAQSLRNDLKDLS
                     GELFFDEAILQTAADDFGHVVHKKPTAVLRPRDAQDIAKLVQFANRQGLKVAMRGQGH
                     SFFGQTQVAGGVVVDSSSLNAVRIIKSGAGGTAEIGPGSKWHPVLMASNAQKLTVPVI
                     TDTFLSVGGTISTGGFGVTTYNLGLQIDHVQELEVVTGDGQIVTCSDERNSDLFNAML
                     GGLGQCGIMTKVVVRLMAAPTNVLFIKMDYDDFQSASADLALLAKDGRFHHLDGRGAA
                     RPTGGVGYYVEGGAFYDEPNDPDEAKLTAGLKFAKKTTTVMSYEQY"
     gene            complement(1075..>1582)
                     /locus_tag="VM910_00010"
     CDS             complement(1075..>1582)
                     /locus_tag="VM910_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVI60970.1"
                     /translation="NKILLATAAFALVAAAAPLCAQQATTKDQLIGSWKVLSLKATTG
                     DKVSYPLGEHVTGYVTVTPDRFWLLFVDATRQAPAAATLTDLEAVAMMKSHVAWTGKY
                     STAEQTPEGIKLTARVDAASSQAIFGTDRVYFMRLDGNRLMVKSPGVIVPMTGATSVV
                     EFELVKAD"
BASE COUNT          301 a          474 c          464 g          343 t
ORIGIN      
        1 aaatactgct cgtagctcat cacggtggtg gtctttttcg cgaacttgag ccctgcagtc
       61 agcttcgcct cgtcgggatc gtttggttcg tcatagaagg cgcccccttc gacatagtat
      121 ccgacgccac cggtcggccg ggcggcgccg cgaccgtcga gatgatggaa gcgcccatcc
      181 ttcgcgagga gcgcgagatc ggcggatgcg ctctggaagt cgtcgtagtc catttttatg
      241 aagagcacat tcgtgggcgc agccatcagc cgcaccacga ctttcgtcat gatgccgcat
      301 tggcccaggc ccccgagcat ggcgttgaag agatcgctgt tacgctcatc ggaacacgtc
      361 acgatctgac cgtcgccggt cacgacctca agctcctgca cgtggtcgat ctgaagacca
      421 aggttgtacg tggttacgcc aaatcccccg gtgctgatcg ttccgccgac tgacaggaat
      481 gtatcggtaa tcactggcac cgtgagtttc tgggcgttcg aggccatgag gacagggtgc
      541 cacttagagc cgggaccgat ctccgcggtc ccgcccgcgc ccgatttgat gatgcggacg
      601 gcattcaggc tgctggaatc gacgaccacg cctcccgcaa cctgggtctg cccgaaaaac
      661 gaatgcccct ggccgcgcat cgcgacctta agtccctggc ggtttgcgaa ctgcacgagc
      721 ttcgcgatgt cctgcgcatc gcgaggcctg agcacggcgg tcggcttctt atgcacgacg
      781 tgtccaaagt cgtcggctgc cgtttgcaag atggcttcat cgaagaacaa ctctccgcta
      841 agatccttta ggtcattgcg cagcgactgt gctgcggcca ggcagttcca cgccggttga
      901 agcgcaagta gtgctgcacc gcctgcgagc cgtttgccga agtcgcggcg agagattttg
      961 cgctgcatga tacacctctc tagtgcgatg cttgtttctg atgggtgcct gacatggacc
     1021 ctacgttaat cccggggcgg aggcttaaag gcgggatcgc gatgatccct taggttagtc
     1081 ggccttcaca agctcaaact cgacgacact tgttgcgcca gtcatcggaa caatgacacc
     1141 cggtgatttc accatcaacc tgttgccgtc gagccgcatg aagtatacgc gatcagtgcc
     1201 gaagattgcc tgactggatg cagcgtcaac acgggcggtc aacttaatgc cttctggcgt
     1261 ctgctcggct gttgagtatt tgcctgtcca agccacgtgc gatttcatca ttgcgacggc
     1321 ttcgagatcg gtcagcgtcg ctgcggccgg tgcttgccgc gtcgcgtcaa cgaacaacaa
     1381 ccaaaatcgg tcaggggtga ccgtaacgta gcccgtcaca tgctccccaa ggggatagct
     1441 cactttatcg ccagttgtgg ctttgaggct gaggaccttc cagctcccga taagctggtc
     1501 cttagtcgtc gcctgctggg cgcacaacgg cgccgctgcg gcaaccagtg caaaggcggc
     1561 cgtggcgagc agtatcttgt tc
//
LOCUS       DATLJT010000002         2118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2067729,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000002 DATLJT010000000
VERSION     DATLJT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067729"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VM910_00015"
     CDS             complement(<1..631)
                     /locus_tag="VM910_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI60971.1"
                     /translation="MKLTRRKFLRLAAGAAALPGASRIACAQSYPARPVRIVVPVAPG
                     GALDILARLIGQWLTERLGQPFVIENRPGAGTNIGVEAVVRAHADGYTLLLIPQSVTT
                     NATLYEKLSFNFVRDIVPIAMISSLPLVMEVALSVPAKTVPEFIAYVKSNPGTISMAS
                     GGSGSMSHVGGELFKLMTHIDMLHVPYRGGAPALTDLIGGQVQVMFSPLP"
     gene            complement(667..1671)
                     /locus_tag="VM910_00020"
     CDS             complement(667..1671)
                     /locus_tag="VM910_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI60972.1"
                     /translation="MELPRRRLLSLAAGAVALPTVLRIARAQVARAQVYPSRPVTIIV
                     PVPPGGVADPIARILADHLTVTLGQPVVVENVSGAGGSIGVGRAARAAPDGYTVSIGN
                     WLSHVGASAVYPVQYDVLKDFEAVSLLTNSPILITARKDFPASDLKQLIAWLKANPDK
                     ASAATVGAGSASHVSGVYFQRATGTHFQFVPYRGGGPAVQDEVAGHVDLMFNEATGAL
                     PYVLSHQIKTYAVLAKRRWFAAPEIPTSEELGVLGVNISFWHGLWVPKGTPKEIVATL
                     NAAVVAALADPTVRKRLTDIGQEIFPRDKQTPEALYAFHRDEIEKWWPIIKAANIRAE
                     "
BASE COUNT          393 a          661 c          664 g          400 t
ORIGIN      
        1 cgggcagagg gctgaacatc acctgcacct gcccgccgat caggtcggtg agtgcgggtg
       61 cgccaccgcg atatggcaca tggagcatgt cgatatgagt catcagctta aacagctcgc
      121 cgccgacatg ggacatactt ccgctgccgc cagacgccat gctaatcgtg cccgggtttg
      181 atttgacgta ggcgatgaat tccggaaccg ttttggccgg aaccgagagg gccacttcca
      241 tcaccagcgg caagctactg atcattgcga tcggaacgat gtcgcggacg aaattgaagc
      301 tgagcttctc atacagcgtc gcgttggtcg ttaccgactg gggtatcagg agaagcgtgt
      361 agccatccgc gtgcgcacgc acgaccgcct cgaccccgat attggtaccc gcgccgggcc
      421 ggttctcgat aacgaagggc tgtccgagcc gctccgtcag ccattgacct atcagtcgcg
      481 caaggatgtc gagggctccg ccaggagcga cgggcacgac gatacgcacc ggccgcgccg
      541 gatagctttg cgcgcaggcg atccgcgacg cgccaggcag cgcggcagcg cccgcggcca
      601 ggcgcaagaa tttgcggcgg gtaagcttca tggcgctcca gtgcctgcaa tgccggcaag
      661 ccggttttac tctgccctga tgttcgccgc cttgatgatc ggccaccact tctcgatctc
      721 gtccctatgg aaggcgtaga gcgcttctgg agtctgttta tcgcgcggga atatctcctg
      781 gccaatatcg gtgagccgct tgcgaaccgt cgggtcggcg agcgcggcga ccaccgcggc
      841 gttgagcgtt gcaacgatct ctttcggcgt gcccttgggg acccacagcc cgtgccagaa
      901 tgaaatattg accccgagca cgccgagctc ctcgctggtc gggatttccg gcgccgcgaa
      961 ccagcgcctc ttggccaata cagcgtaggt cttgatttgg tggctgagca cgtagggcag
     1021 cgcgcccgtc gcctcgttga acatcaggtc gacatggccg gcgacctcat cctgaacggc
     1081 ggggccgccg cctcgataag gcacgaactg gaaatgggtg ccggtcgcgc gttgaaaata
     1141 gacgccgctc acatgcgacg cgctgccggc cccaacggtc gcggccgatg ctttgtcggg
     1201 gtttgccttg agccaagcga tcagttgctt gagatcgctc gccggaaaat ctttcctggc
     1261 tgtgatgagt atcgggctgt tggtcaacag cgacaccgct tcaaaatcct tgagcacgtc
     1321 gtattggacc gggtacacgg cgctcgcgcc tacatggctg agccaattgc cgatactgac
     1381 cgtatagccg tccggcgccg cgcgtgcagc gcggccgacc ccgatgctgc ccccagcgcc
     1441 gctgacgttc tcgaccacca cgggctggcc gagcgtgacc gtcagatgat cggcaaggat
     1501 acgcgcgatc gggtcggcga ccccgccggg tggtacgggc acgatgatgg tgacggggcg
     1561 cgatggatag acctgggccc tcgcaacctg ggccctcgca atccgcaaga cggtcgggag
     1621 cgcgacagcg cccgctgcca gagacagaag ccgacggcgc ggaagttcca cagtggtccc
     1681 aattgccttt gcaagcgcag gttatcacgc gctgcaaaca attcgagagc tttgcggtcg
     1741 gccgttcgga ctggtttgcc tgatgtccgc tccgccccca aaagcggcca aatagcagac
     1801 gtctcgattt gtccgctttg tgcaagagcg gatgctccgg tgacgcccgg gcaagttttc
     1861 acccccattc gtaatgtgga actgctgcag gtgcggcatt ttggtcgttg ccaagccggt
     1921 cagaaatctt cgtcgccgcg ctgacccgtg ccacaatgag cggcaagagc cggcatgcga
     1981 cgaatcgtcg tgacccgtcg acaaaaatcc agaggggaaa tgccatgctc aagcgtgcac
     2041 tcagcacatt tgcgttcgcc gctgcgctga gctttgcgag ctccgctgca tgtgtctggc
     2101 tactggcgat ctcgactc
//
LOCUS       DATLJT010000003         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_689283,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000003 DATLJT010000000
VERSION     DATLJT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689283"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VM910_00025"
     CDS             <1..317
                     /locus_tag="VM910_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI60973.1"
                     /translation="EPLRVAVLGTGGLSHSIGEPTMGWIDEEFDHACIKHFEDGEDAR
                     LATFLTDALARTGNGAHEIRDWVIAHAAAGSKGFELIDYFPSPQTLVGAGFASWRVTG
                     AA"
     gene            451..>2068
                     /locus_tag="VM910_00030"
     CDS             451..>2068
                     /locus_tag="VM910_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI60974.1"
                     /translation="MVVTAPYPAEPPHDSPTLSGRPAAFGERISGYIASRLFITIILV
                     LLVLAGLPIAVWLDLRNLSERALTEQADELSSTIDSIRNYYASNVVGRVLAGGEKTHV
                     LPNYAEVPGAIPIPATLSLELGDLINRNNGNTQFRFFSDYPFKYRPPHAFDEFEQQAL
                     ASLRQNPHSRVYGVSGSIFDRRVRLATPIIMGAACVNCHNTHPDSPKRDWQVGDVRGI
                     EEFVISQPIAAHIFAFKYLLLYFAFVAVTGLAFIALQRHQSSLIARFNKELGQANEFL
                     SALAKKIAKYLPPQLYRGIFAGKDVEIATERKKLTIFFSDIIDFTATAERMQPEELTA
                     LLNEYLTEMSSIAAAHGGTVNKFIGDAILVFFGDPESRGVVRDAKACLAMALDMQRRL
                     AELNVQWRKRGIEEPFRVRMGINTGFCNVGNFGSDDRMDYTIIGAEANLAARLQSIAE
                     PGGIVLSYETFMLVRDMVRARPLEPITLKGIAHPVVPYAVEGSIAAAGKAAQVINEHD
                     TGLDLFIDTDAIDASAAERVRRTLENVVAEL"
BASE COUNT          395 a          683 c          634 g          356 t
ORIGIN      
        1 ccgagccgtt gcgggtggcg gtgctcggca ccggcggctt gagccactcg atcggcgagc
       61 cgaccatggg ctggatcgac gaggaattcg atcacgcctg catcaagcac ttcgaggatg
      121 gcgaggatgc gcggctcgcc acgttcctga ccgatgcgct tgcgcgcacc ggcaacggcg
      181 cgcacgaaat ccgcgactgg gtgatcgccc acgccgctgc cggcagcaag ggcttcgagc
      241 tgatcgacta tttcccgagc ccgcagacgc tggtcggtgc cggattcgcg tcgtggcggg
      301 tgacgggggc ggcctagccg gtcgacagcc ggaccgttcg atcccgaagc tcacacgaaa
      361 gtgtgccgcg agaggttgcg aacgtcgcaa tcgggacact aggtgccccc gcaaggcgat
      421 aacgccgaag aacgcgaaag cgaggccgag atggtcgtaa ctgcgcccta tcccgcggaa
      481 ccgccccacg attctccgac gctatccggc cgaccggccg ccttcggaga gcgaatcagc
      541 ggatacatcg cgagcaggct tttcatcacc atcatcctcg tgttgctcgt cctggccgga
      601 ctgccgattg cggtctggct cgacctgcgc aacctgtcgg agcgcgcgct gaccgagcag
      661 gccgacgagc tgtcgtcgac gatcgacagc attcggaact attacgccag caacgtggtc
      721 ggccgcgtgc tcgccggcgg cgagaagacg cacgtgctcc ccaactacgc ggaagtcccc
      781 ggcgccattc cgatcccggc caccctctcg ctcgagctcg gcgacctgat caatcgaaac
      841 aacggcaaca cgcaattccg ctttttctcg gactatccct tcaagtaccg cccgccgcat
      901 gccttcgacg agttcgagca gcaggcgctg gcaagcctgc ggcaaaatcc gcattcgcgc
      961 gtgtatgggg tttcaggttc gattttcgac cgccgcgtcc gcttggccac gccgatcatc
     1021 atgggtgccg cctgcgtgaa ttgccataat acgcaccccg acagccccaa acgcgactgg
     1081 caagtcggcg acgtgcgcgg catcgaggag ttcgtcatca gccagccgat cgcggcacac
     1141 atcttcgcat tcaaatatct gctgctttat ttcgccttcg tcgccgtcac cggcctcgcc
     1201 ttcatcgcac tgcaacgcca ccaatccagc ctgatcgcgc gcttcaacaa ggagctggga
     1261 caagccaacg agttcctctc ggccttggcg aaaaaaatcg ccaaatatct gccgccgcag
     1321 ctctatcgcg gcattttcgc cggcaaggac gtcgagatcg cgaccgagcg caagaagctg
     1381 acgatcttct tctcggacat aatcgacttc acggcgaccg ccgagcgtat gcagccggaa
     1441 gaactcacgg cgctgctaaa cgaatatctg accgaaatgt cgtcgatcgc ggcggcgcac
     1501 ggcggcacag tcaacaaatt catcggcgac gcgatcctgg tgttttttgg tgatcccgaa
     1561 tcgcgcgggg tggtcaggga cgcaaaggcg tgcctggcga tggccttgga catgcagcgc
     1621 cgcctcgccg agctcaacgt gcaatggcga aagcgcggca tcgaggagcc cttccgcgtg
     1681 cgtatgggca tcaacaccgg cttctgcaac gtcggcaatt tcggcagcga cgaccgcatg
     1741 gattacacga tcatcggcgc cgaggccaat ctcgccgcgc gtctgcagtc gattgcggag
     1801 ccgggcggga tcgtgctgag ctacgagacc ttcatgctcg tgcgcgacat ggtgcgtgcg
     1861 cgcccgctcg agccgatcac gttgaagggc atcgcccacc cggtcgtgcc ctacgccgtg
     1921 gagggcagca tcgccgcggc gggcaaagct gcgcaagtca tcaacgagca cgataccggg
     1981 ctcgacctct tcatcgatac cgatgcgatc gatgcgtcgg cggcggagcg cgtgcggcgg
     2041 acgctcgaaa acgtcgtcgc cgagttga
//
LOCUS       DATLJT010000004         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_919020,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000004 DATLJT010000000
VERSION     DATLJT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919020"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="VM910_00035"
     CDS             <1..150
                     /locus_tag="VM910_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase"
                     /protein_id="HVI60975.1"
                     /translation="SMRNIRESLAKMATGLLPVIDTEVALADFERGLARIESRQVFGK
                     IIVTF"
     gene            173..1105
                     /locus_tag="VM910_00040"
     CDS             173..1105
                     /locus_tag="VM910_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HVI60976.1"
                     /translation="MTKLGALAHRVRRHVKAGVDTALGSLAVGMLRTIRATNRKHMAD
                     FAGSFMRRLGPRLKEHKIGRANLAAAFPEKSPAEIETILRGVWDNLGRVAAEFAHIDR
                     LQMFDPDPQQMGDIIYSSEVDKRFRQLRCDGKPALVFAAHLANWELPALIAAKYRLET
                     TVLYRQPNIAAVSDAIIQIRKGSMGTLVPTSLDAPFKLIRVLEAGGHVAMIVDQHYVN
                     GVDVTFFGRRCKANPFIARLARHVDCPIHGTRIIRLPDRHRFRVDLTEAIAPPRDADG
                     KIDVEGTMQAITSVIEGWVREYPEQWLWVHRRWR"
     gene            complement(1131..>2069)
                     /locus_tag="VM910_00045"
     CDS             complement(1131..>2069)
                     /locus_tag="VM910_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI60977.1"
                     /translation="RYDTFDSNDTLETKLLAGKSGYDVVVPTAYFLERQIKAGVFQKL
                     DKAKLPNLANMWPEISQRLAVYDPGNQYAVNYMWGTTGIGYNVKKARDALGANGKIDS
                     WDIVFKPENLAKFKDCGVHLLDSSDDILAAALHYLGLDPNTTKEADLQKAADLVTKIR
                     PYVRKFHSSEYLNALASGEICLVVGFSGDVKQSQKRALEAKNGVEIAYAIPKGGAQLW
                     FDNLGIPRDAKNVAEADAFINYLQKPEVAAKNTNFISYANGNLASQKFIDKSILDDKT
                     IYPDEATMKELYIINAHDPKTQRLMNRLWTRIKTGK"
BASE COUNT          380 a          633 c          639 g          417 t
ORIGIN      
        1 tcgatgcgca acatccgcga gagcctcgcc aagatggcga ccggcctgtt gccggtgatc
       61 gacaccgagg ttgcgctcgc cgacttcgag cgcgggctgg cgcggatcga aagccggcag
      121 gtgttcggaa agatcatcgt caccttttga cgagccgccg cgagcatcgc ctatgacgaa
      181 gttgggagct ctcgcccatc gggtccgccg acatgtcaag gcaggcgtcg ataccgcgct
      241 cggatcgctg gcggtgggaa tgctgcggac gatccgcgcc accaaccgca agcacatggc
      301 cgacttcgcc ggcagcttca tgcgcaggct tggtccgagg ctgaaggagc acaagatcgg
      361 ccgcgccaat ctcgcggccg cctttccgga gaaatccccg gccgagatcg aaacgatcct
      421 gcgcggcgtt tgggacaatc tcggacgcgt cgcggccgag ttcgcccaca tcgatcgcct
      481 gcaaatgttc gaccccgacc cgcagcagat gggcgacatc atctacagca gcgaagtcga
      541 caaacggttt cggcaattgc gctgcgacgg caagccggcc ttggtgttcg ccgcccatct
      601 cgcgaattgg gaactgccgg cgctgattgc ggccaagtat cggctcgaga cgacggtgct
      661 gtaccgccag cccaatatcg ccgccgtgag cgatgcgatc atccagatcc gcaaaggcag
      721 catgggcacc ctcgttccga ccagcctcga cgcgccgttc aagctcatac gcgtgctcga
      781 agccggcggc cacgtcgcca tgatcgtgga ccagcattac gtgaatggtg tggacgtgac
      841 cttcttcggg cgccgatgca aagcgaaccc cttcatcgcc cgcctggcgc ggcacgtcga
      901 ttgcccgatt cacggcacgc gcatcatccg cctgcccgac cgccatcgct ttcgcgtcga
      961 tctgaccgaa gctatcgcgc cgccacgcga tgccgacggc aagatcgacg tcgagggcac
     1021 gatgcaggcg atcacgtcgg tgatcgaagg ctgggtgcgc gagtacccgg agcaatggct
     1081 gtgggtgcat cggcgctggc gatgacgaga gcgtcgctcg aatctcgcgc ttacttgccg
     1141 gtcttgatcc tggtccacag ccgattcatc agccgctgcg tcttcgggtc gtgggcgttg
     1201 atgatgtaaa gctccttcat ggtcgcctcg tccggataga tcgtcttgtc gtcgaggatc
     1261 gacttgtcga tgaatttctg gctcgccaga ttgccgttgg cataggaaat gaagttcgta
     1321 ttcttcgcgg ccacctcggg cttctgcaga taattgatga acgcgtcagc ttcggccacg
     1381 ttcttggcat cgcgcgggat gccgagatta tcgaaccaca attgcgcgcc ccccttcggt
     1441 atggcgtagg cgatctcgac accgttcttg gcttccagcg cgcgcttttg cgactgcttg
     1501 acgtcgccgg aaaagcccac cacgaggcag atctcgccgg aggcgagcgc gttgagatac
     1561 tccgaggaat ggaatttgcg cacgtagggc ctgatcttgg tcacgagatc ggcggccttt
     1621 tgcagatcgg cctctttggt ggtgttgggg tcgagaccaa gatagtgcag cgctgccgcc
     1681 aggatgtcgt cggaggaatc gagcaaatgc acgccgcagt ccttgaactt ggccaagttc
     1741 tcgggcttga agacgatgtc ccagctatcg atcttgccgt ttgccccaag cgcatcgcgt
     1801 gcctttttga cgttgtagcc gatgccggtc gtgccccaca tgtaattgac ggcgtactga
     1861 ttgccggggt cgtagaccgc caatcgctgc gagatctccg gccacatatt ggcgaggttc
     1921 ggcaacttcg ccttgtccag tttctggaaa acaccggcct tgatctgacg ctcgagaaaa
     1981 taggcggtcg gcaccaccac gtcgtagccg gatttgccgg ctagcagctt ggtctcgagc
     2041 gtgtcgttgg aatcgaaagt gtcgtagcg
//
LOCUS       DATLJT010000005         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4135488,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000005 DATLJT010000000
VERSION     DATLJT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135488"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VM910_00050"
     CDS             <1..136
                     /locus_tag="VM910_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein usg"
                     /protein_id="HVI60978.1"
                     /translation="PALLDFLTFWQEKLEGPLFRVTVAHSRLIKPAELRAVDGVFRLH
                     "
     gene            230..763
                     /locus_tag="VM910_00055"
     CDS             230..763
                     /locus_tag="VM910_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI60979.1"
                     /translation="MARKKPTSRAAARTAAQKKSPARKAGAKSTARKTIAGKGRAQTS
                     VKRARPKQKIAISHHREEDFKADGLRAYAKYRDLGIADATGGLARAHVIRLMGPCNPE
                     EVSKLHYHDVEFQMVYVLKGWVKTYMEGHGEMLYEQNSSWTQPPRIRHMILDYSDDVE
                     LLEVILPADFKTVELVA"
     gene            831..2834
                     /locus_tag="VM910_00060"
     CDS             831..2834
                     /locus_tag="VM910_00060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVI60980.1"
                     /translation="MTSSFRVPGVTMTRALDQIVSPHKGNEARALTFGYREDIDWLRA
                     IAVLAVVAFHFEAPAVFGGFVGVDIFFVISGYLITGIILSELKAGNFSFARFYERRVR
                     RLLPALYAMVALTAIPSFHFLLTSERAEFFRSIASVVTFTSNFFFWFQTGYFDHAAVE
                     KPLLHTWSLAVEEQFYLALPITLFLLSRFSRGGRAALPIAFGTLTLASFGLSVWLMRT
                     GGSANAFFMSPPRAWEFLVGGIVALPGFPVLRHAFARRAVRGAAIAMLAIPIFSLRQG
                     PGFPGINALSPCIGAALFIWSGIGVPTRVRRSYSPLNVARFFGQISYSLYLWHWPLFA
                     FVRFSKDSLVLGASDKIALFALTVAISWVSWRFVEQPFRNRTLAPTRGSAFRLAFASS
                     FVLLIASAGGLAISRNPSAVDEAAVRFDSYNSYEFAPLYRNGSCFNPPSGIFGDDCLV
                     PAAGKANLLLWGDSLAAHYFYGLSRTVDAQRLNVLQATQAACMPTLNAATQSIATCRD
                     FDVQMTAYFKEHKPDLVIMSADWLEYSRSGQFDSMISDIRTTISRLNAAGIRIALIGP
                     NVQFRSRLPSMLARARLRGVEARSDDVLVPEVFAFDRSMQAALPDTEGFRYVSVANAV
                     CPARQCPLLVGDNVPLAWDHAHLTAEGSIYVAEKLLPLLGLDR"
     gene            complement(2843..>3560)
                     /locus_tag="VM910_00065"
     CDS             complement(2843..>3560)
                     /locus_tag="VM910_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HVI60981.1"
                     /translation="MTGGMAGIAGTVLVLYATLLAPLIPDAAAHFVIASVLGAPAAIL
                     VSLIMVPETTDRLTGGALDDPEMQASSTMDAIVKGTASGLELLLNIIAMLIVLVALVH
                     LANAVLGLLPDVGGSGISLQRMLGLLMAPVCWLMGLPWPQAVTAGSLMGTKTVLNELI
                     AYVDLSKLAPDALDARSRLIMLYAMCGFANFGSLGIMIAGLGTMAPERRHEINSLGLK
                     SIVSGTLTTCLMGAIVGLLT"
BASE COUNT          621 a         1115 c         1106 g          718 t
ORIGIN      
        1 tccggcgctg ctggattttc tcacgttctg gcaggagaag ctcgaaggcc ccttgttcag
       61 ggtgacggtg gcacactcga ggctgatcaa gccggctgag ctgcgcgccg tcgacggcgt
      121 gttccgcctg cactgatcct ctcttcctca ggcgtttgat cgcgctccgc tgtgcggagc
      181 ctgcgaacgt gctaaccttt cgaaccggaa accgaagggg agagcactga tggccaggaa
      241 gaagccgacc tcacgcgcgg cggcgagaac tgccgcacag aagaaatcgc ccgcgcgcaa
      301 ggcgggtgcc aaatccaccg cgcgcaagac gatcgcgggc aaggggcggg cgcagaccag
      361 cgtgaagcgc gcgcggccga agcagaagat cgccatcagc catcaccgcg aggaggattt
      421 caaggccgac ggactgcgcg cctatgcgaa gtatcgcgac ctcggcatcg cggacgcgac
      481 cggaggcctt gcgcgcgccc atgtgatccg cctgatgggg ccgtgcaacc ccgaggaagt
      541 ttcaaagctg cattatcatg acgtcgaatt tcaaatggtc tatgtactca agggctgggt
      601 gaaaacctac atggaaggcc atggcgagat gctgtacgag caaaacagct catggaccca
      661 accgccgcgc atccgccaca tgatcctcga ctattccgac gatgtcgaat tgctcgaagt
      721 gattctgccc gcggacttca agacggtcga actcgtcgcg tgagccgcgg tcatcctggg
      781 gccgtgcctg ggcgttcaac ccggaatctc ggagtcgtct gcgagctgga atgacatcga
      841 gtttccgggt accaggcgtc acgatgacgc gcgctttgga tcaaatcgtt tcccctcaca
      901 agggaaacga agcaagggct ttgaccttcg gctatcgcga ggacattgac tggctgcggg
      961 ccatcgccgt gctggcggtg gttgccttcc atttcgaagc acccgcagtg ttcggcggct
     1021 ttgtcggcgt cgacattttc ttcgtgatat cgggctatct gatcaccggc atcatcctgt
     1081 cggaactcaa ggccggcaat ttctccttcg cgcgcttcta cgagcggcgc gtgcggcggt
     1141 tgctgccggc gctctatgcc atggtcgcgc tgacggcgat tccatcgttc catttcctgt
     1201 tgacgtccga gcgcgcggaa ttctttcgct cgatcgcgtc cgtcgtgacg ttcacgtcga
     1261 acttcttctt ctggtttcaa accggctatt tcgaccacgc cgcggtggag aagccgctgt
     1321 tgcacacatg gtcgctcgcg gtcgaggagc agttttatct ggcactgccg atcaccctgt
     1381 tcctgctctc gcggttttcg cggggagggc gcgcggcgct gccgatcgcg ttcggcacgc
     1441 tgacgcttgc ttccttcggc ctgagcgtat ggctgatgcg gacgggcggc tccgccaatg
     1501 ccttcttcat gagcccgccg cgtgcgtggg aatttctggt tggcggcatc gtcgcgctgc
     1561 cgggttttcc tgtgctgcgc cacgcgtttg cgcggcgggc ggtgcggggc gctgctattg
     1621 cgatgctggc aataccgatt ttttcgttgc ggcagggacc gggatttccc ggcatcaacg
     1681 cgctgtcgcc ctgcattggg gcagcgctgt tcatctggag cggcatcggc gtaccgacgc
     1741 gcgtgcgcag aagctattcg ccgctcaacg tcgcaagatt cttcggccag atttcctatt
     1801 cgctttatct atggcattgg ccgttgtttg cctttgtccg cttttccaaa gacagcctgg
     1861 tgctgggagc gagcgacaag atcgcacttt tcgcgctcac ggtcgcgatc tcctgggtct
     1921 cctggcgatt cgtcgagcag ccgttccgca accgcacgct cgcgccgacg cgcggctccg
     1981 cgttccgcct ggctttcgcg agctcattcg ttctcttgat cgcgagcgcc ggcgggcttg
     2041 ccatcagccg caacccatcg gcggtcgacg aggccgcggt gcggttcgat tcctataatt
     2101 cctacgaatt cgcgccgctc tatcgcaacg gaagctgctt caatccgccg agcgggatct
     2161 ttggcgacga ttgcctggtt ccggccgcgg gaaaagccaa tctgctgttg tggggcgaca
     2221 gcctcgccgc tcattatttc tatgggctga gccggaccgt cgacgcgcaa aggctgaacg
     2281 tcctgcaggc cacacaggcc gcgtgcatgc cgacgctgaa tgcggcgacc cagtcgattg
     2341 cgacgtgccg cgacttcgac gtgcaaatga cagcctattt caaagagcac aagccggatc
     2401 tcgtcatcat gtccgcggat tggctggaat attcgcgatc ggggcagttc gacagcatga
     2461 tatccgacat caggacgacg atttccaggc tcaacgcggc cggcattcgt atcgctttga
     2521 tcggacccaa cgtgcagttc cgaagccggc tgccgtcgat gctggcgcgg gctcgcctgc
     2581 gcggcgtcga ggcacgttcc gatgacgtcc tggtgccgga ggtctttgcg ttcgaccggt
     2641 cgatgcaggc tgcgctcccc gataccgagg gctttcggta cgtttccgtc gccaacgcgg
     2701 tctgccccgc gcggcaatgt ccgctccttg tcggcgacaa cgttccgctc gcctgggatc
     2761 acgcccatct caccgcagag ggatcgatct atgtggctga gaaacttctg ccgctactcg
     2821 ggcttgatcg ataggcggtg catcacgtca gcaggccgac gatcgctccc atgaggcaag
     2881 tcgtcagcgt gcccgacacg atcgatttca accccaacga attgatctcg tggcgccgct
     2941 cgggcgccat cgtgccaagg cctgcgatca tgatgccgag gctgccgaaa ttggcaaagc
     3001 cgcacatcgc atagagcatg atcagcctgg agcgcgcatc gagcgcgtcg ggtgcaagtt
     3061 ttgagagatc gacataggcg atcagctcgt tcagcacggt cttcgtaccc atcaggcttc
     3121 cggcggtgac ggcctgcggc cagggcaggc ccatcagcca gcacaccggc gccatgagaa
     3181 gaccaagcat ccgctgcaac gagatgccgc tgccgccgac gtcaggcaac aggccgagca
     3241 ccgcattggc gaggtgcacc agcgccacca gaacgatcag catggcgatg atattgagca
     3301 ggagctcgag tccgcttgca gtgcccttga cgatggcgtc catggtgctg gaggcctgca
     3361 tctccggatc atcgagcgcc ccgccggtca gacgatcggt tgtctccggc accatgatga
     3421 ggctcaccaa aattgctgcc ggcgcgccca gcacggatgc gatcacgaaa tgcgccgcgg
     3481 cgtccgggat gagcggcgcc agcagcgtgg catagagcac gagcacggtg ccggcgatcc
     3541 ccgccatgcc gccggtcatc
//
LOCUS       DATLJT010000006         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1838043,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000006 DATLJT010000000
VERSION     DATLJT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838043"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..1593
                     /locus_tag="VM910_00070"
     CDS             802..1593
                     /locus_tag="VM910_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI60982.1"
                     /translation="MVRITKTSRPKKALGRKARRASKSLEPARRVSENKPIDLYYWPT
                     PNGWKITIMLEECSLPYTMVPVDISKGDQFKPQFLAISPNNRMPAIVDHDGPDSAPIS
                     IFESGAILQYLGRKTGKFYPRDERGRVEVEQWLFWQMANLGPKAGEANHFRHYAPEKL
                     PYAIGRFSNEMNRIYGVMNTRLKDREFLAGNYSIADIACVGWASRAERHGHDLSGFPN
                     VRRWLQTLLARPAVKRGLSVKVEAAFHVDMKDPNVRSVLFNQKAR"
     gene            1875..>2729
                     /locus_tag="VM910_00075"
     CDS             1875..>2729
                     /locus_tag="VM910_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HVI60983.1"
                     /translation="MPALTVAAVRKTAAQIHRQEVRDTLAPGLYLVVQPKPSGAKSWA
                     LRFRRPDGRPAKLTLGPVDLGEKETAEAPVLGAALTLRQARELANQIDRARVRGVDVI
                     AERKQSAGTEFGHAVRQFFSDYKTKRHTRPRRWRDDARLLGLRWPVGADPATHAPEVI
                     KGGLAATWASKPVAAIDGAAIHSLVEAARREGIPGLARRNGSTSETRGRKMHAALSIL
                     FRWLLQRRLVTSNPTVGVWHPGAPPARDRALTDHELRAFWRACEQAGAPYGPLLQVLL
                     LTGCRLNEV"
BASE COUNT          535 a          881 c          861 g          452 t
ORIGIN      
        1 gctgcaaaaa cactggtttt tttatcacgc tgtagtaatc ccaatgcagt gttcgcgatc
       61 cccgcgggca cggacgacat cgccactcac tcacggctag gagggcagca tggcgaagaa
      121 gcgaaggaag atggcgaagg ccgcgaaaaa gggcgctaaa aagaccaagc gccggaagaa
      181 gaagtaaccg cgcgccgcgc atcagtcttc gatttcgaac gacgtcgatg actgcgtcaa
      241 acgggccggc gcaagtcgaa gagcggtaag cgcgcagcgc gtcgcacttc gaaatcgccg
      301 gccccggtag acgacagggg gcgtcggcga gacaggggtc gagcttcatc tgtccccgct
      361 tggcggacag tgaagatgag gaaacggaag acctccgtac ctcgccgcgg cacaacattt
      421 gccgacggat tcgtagcccc ggcctctcgc cgacgccctc ggtatttttg gaaaatgcgt
      481 gccgtgtggg cgaggccgga cagtcgtgcg ggctcgcgcc gttttgactg gccgcgattg
      541 ccctcgcccg ttgaggcggg tgcctttttc agacactcac ccatgaattt gcgataggcc
      601 gtgcggccct tcacgccggt cgtggtgagc cgtgtcggcg gcactatgga acgccgaaca
      661 cgatcgatct caacgaagat aagctatccc cccggcacgg cgtccattcg ttgcagcctc
      721 gccgatataa tggttgctgc ctcgtttggt cgcggcttgc caggaggagg cggcacccat
      781 tctgcatcga gggaggtgtc catggtccgt atcaccaaga cgtcgcgccc aaaaaaagcc
      841 ctcgggcgca aggcccgccg cgcgtcaaaa tcgctcgaac cggcacgccg cgtgagcgaa
      901 aacaaaccca tcgacctcta ttactggccg acgcccaatg ggtggaagat cacgatcatg
      961 ctcgaggagt gctcgctgcc gtacaccatg gtcccggtcg acatctccaa gggcgaccag
     1021 ttcaagccgc agtttctggc aatctcgccc aacaaccgca tgccggcgat cgtcgatcac
     1081 gacggccccg acagcgcccc gatctcgatc ttcgagtccg gcgcgatcct gcaatatctt
     1141 ggccgtaaga ccggaaagtt ctatccgcgt gacgagcgcg gccgcgtcga ggtcgagcag
     1201 tggctgttct ggcaaatggc caatctcggc cccaaggcag gggaggcgaa ccacttccga
     1261 cattatgcgc ccgagaagct gccatacgct atcgggcgct tcagcaacga gatgaaccga
     1321 atctacggcg tcatgaacac gcggctgaag gaccgcgaat tcctcgccgg caattattcg
     1381 atcgccgata tcgcttgcgt gggttgggcg agccgggccg agcgccacgg ccacgacctg
     1441 agcggctttc ccaacgtcag gcgctggctc caaaccttgc tggcgcgtcc ggcggtcaaa
     1501 cgcggtctgt ccgtcaaggt ggaagcggcc tttcacgtcg acatgaaaga tccgaacgtg
     1561 cgctcggttc tattcaatca gaaagcgcgc taaacggagg gacacggccc cgctcgaccc
     1621 attgcggtcg tttgaggtcc ttgacggtac tcggcatggc cgatacggac gttgcgttgc
     1681 cggagacgtg gccgagtggc tgaaggcggc ggtttgctaa accgacgccg gccactttca
     1741 acccccaaca cccgccaaca ttttctccct gaaatagtct actaagctgt tgtgcctttg
     1801 ggcaaaatag ccgtatgtta gtaacgcacg ctaacagggc ataacgccct cgcggcgtta
     1861 ccccaggttt acccgtgccc gcattgactg tggcggcggt gcgcaaaacc gcagcacaaa
     1921 ttcacaggca ggaagtccgc gacactctcg cccccggcct gtacttggtg gtccaaccca
     1981 agccgagcgg agccaagtcg tgggcgctgc gcttccgccg gcccgacggg cgccccgcca
     2041 agttgacctt ggggccggta gacctcgggg aaaaggaaac cgctgaggcc cccgtgctcg
     2101 gtgccgcgct gacgttgcgg caagcgcgcg agctcgccaa ccagatcgac cgcgcccgcg
     2161 tgcgcggtgt tgacgtcatc gcggagcgca agcaaagcgc ggggacggag ttcggccacg
     2221 cggtgcggca gttcttcagc gactacaaga ccaagcggca cactcgcccc cggcgctggc
     2281 gtgacgacgc ccgcctgttg ggcttgcgct ggccagtggg tgccgacccg gcaacccacg
     2341 cccccgaagt catcaagggc gggctggcgg caacgtgggc cagcaagccg gtggcggcta
     2401 tagacggcgc cgcgatccac agcttggtgg aggcagcccg ccgcgagggc atccctgggc
     2461 tggcgcggcg caacggcagc accagcgaga cgcgcgggcg caagatgcac gcggcgctct
     2521 cgatcctgtt ccggtggttg ctgcagcggc gcttggtcac cagcaacccc accgtgggcg
     2581 tgtggcaccc cggcgcacca ccggcccgcg atcgagcgtt gaccgaccac gagctgcgcg
     2641 cgttctggcg cgcttgcgaa caagccgggg ccccatatgg cccgctactg caagtgctgc
     2701 tgttgaccgg ctgccgactg aacgaagtc
//
LOCUS       DATLJT010000007         5540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2067873,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000007 DATLJT010000000
VERSION     DATLJT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067873"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..305
                     /locus_tag="VM910_00080"
                     /pseudo
     CDS             159..305
                     /locus_tag="VM910_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1178..1906)
                     /locus_tag="VM910_00085"
     CDS             complement(1178..1906)
                     /locus_tag="VM910_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI60984.1"
                     /translation="MPPAPSLRSAQALSSDVARACREINALTDKIKNVDYEINGLTDQ
                     LKHAKRKAEEYQIAIGRHLKAIKAARPDDWGDIVKAECNLGRTRAYELMAISGGTKTV
                     EQTRAQTNIRKIKHRKSVRSGTDGGTLMADIDAGTADSDAAGVTRPRRTKHESYLEGI
                     RTLARTLEMMCGIPVPRFDLETFGGVIEAIHEVEAALSQFKEKVTREGEPAQEGDAAA
                     LAEQPADVIEPDCDGELVWRSSES"
     gene            complement(2020..2757)
                     /locus_tag="VM910_00090"
     CDS             complement(2020..2757)
                     /locus_tag="VM910_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI60985.1"
                     /translation="MSRKRQPTDVRTAANKWQTHPFATREDWRPGEKDAITGTLMVLD
                     KNRLRVREADFERLLDLLMKDFKIDDRATLKSHVEVATRIYNHNPDKLSDRDRERLVK
                     FAPKLAGLLRKNAIPIVNIITAFDQQHVRGFEPEFCKSIYDQGEADAFMTRIWQASHT
                     LLELADVLSRHHPRGRPPDAGLHGAMTLLRDYWERELGRNLKSDPWKRGFASFLRVVF
                     GFVDPSAIDKLPSAAKRRVRALDKTPR"
     gene            3154..3471
                     /locus_tag="VM910_00095"
     CDS             3154..3471
                     /locus_tag="VM910_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HVI60986.1"
                     /translation="MSKRKPARSIETRSASKAAGSVQPTGRADSKQAWVLALLCGPSG
                     ATIATVTRSTGWQPHTVRAFLAAVVRKKLGLRLESQKTDGERVYRIVAREAYTDATSV
                     PSA"
     gene            3474..4034
                     /locus_tag="VM910_00100"
     CDS             3474..4034
                     /locus_tag="VM910_00100"
                     /inference="COORDINATES: protein motif:HMM:NF022598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HVI60987.1"
                     /translation="MRGQSTNPAAIEAEIAHLHSLALDALRRHWRVTFGRTPPVDLSK
                     DLLGRMIAYRLQERAFGGLDRESLRFLHGLARHGGSPRRRLKPGTVLIRDYHDQRHTV
                     TVVTDGFEWQGATYPSLSAIARAITGTAWSGPRFFALAPANGAPTSRNKNARAREGRD
                     GLQPARGMNGSEMNTASLPLQTAARA"
     gene            4057..4887
                     /locus_tag="VM910_00105"
     CDS             4057..4887
                     /locus_tag="VM910_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVI60988.1"
                     /translation="MRPYWKGYLKLALVSCPIALHAACSSAERIALRQINKATGNRLR
                     QQLIDEETREPVAPEHKGRGYEVAKGQYLIIEDAELEAIEIESTHTIEIDRFVPHSQI
                     DQRFFDSPYYVMPSEPAGQEAFAVIREAMRGKGVVALGRLVLSKRERGIALEPYGKGL
                     LGTTLRYPYEVRKVEDYFCDLPDLTIAPDMLTLAEHILDSKASEFDPATFRDRYEEAL
                     LAHLKAKQAGAVPERRKTFAPPQRVVNLMEALRRSVAEDTKGAAPRKGASATPARKRA
                     "
     gene            5072..>5540
                     /locus_tag="VM910_00110"
     CDS             5072..>5540
                     /locus_tag="VM910_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI60989.1"
                     /translation="MNGPISKLARCAIYTRKSTEYNLELAFNSLDAQREACEAYIKSQ
                     AHEGWRLISGRYDDGAFSGASLDRPALQQLLADVRASKIDIVLVYKVDRLTRSLADFA
                     KLIELFDTHGVSFVSVTQSFNTSSSMGRLTLNVLLSFAQFERELIGERVRDKIA"
BASE COUNT         1013 a         1738 c         1720 g         1069 t
ORIGIN      
        1 tccgggcacc cactcctcag gcttgatcac ccaatcgggt tgcgttcgtc gcctcgccgt
       61 tccgcctccg gtccgctacc ttttcgaacg cctcgcccgt gtcggcaaga ataacggctg
      121 cgcccgtttc cgcctcatag cttcacgccg gcgttcttgc tggcctcatt ctcaagggtg
      181 ccaaacccgc cgatctcccc gtcgtgcagc ccgcaaaatt tcagctggtc atcaattcga
      241 acacggcgag gcagcttggg ctgacgcctc ccccagacct gatcccggac gagctgatcg
      301 aataaccaaa gccggcgctg ccttctgtcc gctgctggcg gaagcagaca taaacagccc
      361 aacagacacc aaggaggagg cgcttgatat agcgccgcct ggccctgcac cgaaagtgac
      421 ggattcagtg agctacaaac acacgttggt cacggtcaag tggtgacaga tgcgttgctt
      481 tgcggcctcg ccggttcgcc gatcgcgcgg cttggcgcca gagtctcgct tggtagcacg
      541 gtcaagcaag gtcgggccac gatgggaagg cccagaaaaa ccgagcggcg cggcgcccgg
      601 agatggacag caattcttgg tcgatggccg gcggatcacg aggtgccgcc aactgttcaa
      661 gtcgagccgg catgttggct ggtagagacc cgactgcatg gctgggcatg taggactcga
      721 actcagaaat gtcgacataa actatccctt tgaaacaccg tatagatctg ttggggtcca
      781 gccgaattcc cggcacagag accattcgcg tttgagctgc cgcgccgggg gtacgcagct
      841 caggctcagt gccaacatcc cgcggcgtcc tcgcgcagac ggtgctcatc gatctgaatc
      901 gttgagatga cgcgcgaatt gccacgattt ttgccagcgc aaagagagcg tgtgtggtga
      961 ccacggacag tgcctataag ctggggatgc agcgctcaat ggttgcggtt tgagtgccat
     1021 catcgaccgc gccatggcat gtccggcgtg gcgaggactg gaggcgaggc ctcggcgacc
     1081 tgatcaaggg cggccgataa ggtagttttc gtgctctcgc cgacttcggc gcgctccgaa
     1141 atctgcgcct gggaggtgga ggaccggctg accgagatta gctttcgctg gaacgccaca
     1201 ctaactcgcc gtcgcagtcg ggctcgatca catcggcggg ctgttccgcg agcgcggccg
     1261 catccccctc ctgtgcaggc tcaccttcgc gggtgacttt ctccttaaac tgggaaagag
     1321 cggcctcgac ttcgtgaatt gcctcaatca cgccgccaaa cgtttcgagg tcaaagcgcg
     1381 gaaccggtat cccgcacatc atctccaagg ttcgggcaag agtcctgatg ccctctaggt
     1441 agctctcgtg cttggtcctt ctcggccgcg tcacgccggc tgcatcgctg tccgccgtcc
     1501 ccgcgtcgat atcggccatg agagtgccgc cgtccgttcc ggaacggacg gactttcgat
     1561 gcttgatttt gcggatattg gtttgagcgc gggtctgctc gaccgtcttg gtgccgccgc
     1621 tgatggccat caactcatag gcgcgggtgc gaccgagatt gcactcggcc ttgacgatgt
     1681 ccccccagtc gtccggccga gcggccttga tggccttgag atgccgaccg atggcaatct
     1741 gatattcctc agctttacgt ttagcgtgct tgagctggtc ggtcagcccg ttgatctcgt
     1801 aatcgacgtt cttgatcttg tcggtcagcg cgttgatctc gcggcaggcg cgtgcgacgt
     1861 cgctcgaaag ggcttgcgcg gatcttaggg agggggcagg tggcatggcg tgaacctctc
     1921 atctttgctg ggtgaaggtg gaaatccctg tccgtctatc ttcggacacg gatatgcgcc
     1981 cccgaaaaaa tctaggtaag cggacagaat tggctcgatt tagcggggtg ttttgtccaa
     2041 tgcccgcacg cgccgtttgg ccgcgctcgg caacttgtcg atggcgctag ggtcgacgaa
     2101 cccaaaaacg acgcgcaaga agctggcaaa tccgcgcttc caaggatcag actttaaatt
     2161 tctgccgagt tcgcgctccc aatagtctcg taacaatgtc attgcgccgt gcagtcccgc
     2221 gtccggtggc ctcccccgcg gatggtgccg tgagagaaca tccgccaact ccaggagcgt
     2281 gtgagaggct tgccatatgc gcgtcatgaa ggcgtcggcc tcgccttgat cgtagatgct
     2341 cttgcaaaac tccggctcga agccgcgaac gtgctgctgg tcaaaggccg tgatgatgtt
     2401 cacgatcggg atggcgttct tgcgaagtag gcccgctagt ttaggtgcga actttacgag
     2461 ccgttcgcgg tcgcggtctg ataatttatc ggggttgtgg ttgtagatac gcgtcgcgac
     2521 ctcgacgtgg cttttgagag tcgcgcgatc gtcgatcttg aaatctttca tcagcagatc
     2581 gaggagccgc tcgaagtcag cctcgcgcac ccgcaggcga ttcttgtcga gcaccatgag
     2641 ggtaccggtg atggcgtcct tctcacccgg gcgccagtcc tcgcgggtcg cgaacgggtg
     2701 ggtttgccat ttgttggcgg ctgtccgaac gtcggtaggc tgtcgcttgc gagacatcaa
     2761 ccagccctcc gccgcattgg agtgacgacg ccgccacggg ccatcctttc tttttcttac
     2821 tcgaaattcg aacacgaccg gatctgttcc gttccaatcc acaggacgat agatgcgagc
     2881 ggggccaatt ctcgaggtcg agggctagca tcgagtatgg ccggcgccac tgcccaggag
     2941 gccgagcaaa gccagcaaca ccattgctca gtttgcgctg gactttccgc gcaaagagag
     3001 cgtgtgtggt gatcacggac gccgagcctg accggcttgc tcctctcgcc ccgccagcgc
     3061 cgatgggcct ccgcggggtt tgggtggtgg cggcgcgctg ttgcgccagc cctccggcgc
     3121 gctgttgcgc cgaccccttg agaggagttt tgcatgtcca agcgcaaacc tgcgcgcagc
     3181 attgagacgc ggtctgcatc gaaggcagcc ggcagcgttc agcccacggg gcgagcggac
     3241 tcgaagcagg cgtgggtgct ggcactgctc tgcggaccga gcggcgccac gatcgccacc
     3301 gtcacgcgct ccaccggctg gcagccgcac acggtgcgcg ccttcctggc cgccgtggtg
     3361 cgcaagaagc tcgggctcag gctggagtcg cagaagacgg acggcgagcg cgtgtatcgg
     3421 attgttgccc gcgaggctta caccgatgcc acgagtgtgc cgagtgcgta gccatgcggg
     3481 ggcagtcgac aaacccggcc gcaatcgagg ccgagattgc tcatttgcac tcgcttgcgc
     3541 ttgacgcgct tcggcgacac tggcgcgtga ccttcggacg gaccccgcca gtagatctaa
     3601 gcaaggacct gttggggcga atgatcgctt atcgcctgca ggagcgggcg tttggcgggc
     3661 ttgatcggga aagcctgagg ttcttgcacg ggctggctcg gcacggcggg tcgccacggc
     3721 gccgcctcaa gcccggcacg gtgctcattc gcgactatca cgaccagcgc cacaccgtca
     3781 cagtcgtcac cgatggcttc gagtggcagg gcgcgactta tccgagcctg tccgccatcg
     3841 cccgcgccat caccggcacc gcctggagtg gtcctcggtt ctttgccctc gcaccagcaa
     3901 acggcgcccc taccagccgc aataaaaatg cacgtgcgcg ggagggccgc gatggcttgc
     3961 aacccgcccg cggaatgaac ggctcggaaa tgaacaccgc atctttgccc ctgcaaaccg
     4021 ccgcgcgggc gtaaacctaa aacataggag ttggttatgc gtccctactg gaaaggctat
     4081 ctcaaactgg ctctggtctc ctgtccaatt gcgctccacg cggcttgctc gagtgccgag
     4141 cgcatcgcct tgcggcagat caacaaggcc accggcaatc gcctgcgcca gcagctcatc
     4201 gacgaagaga cccgcgagcc ggtcgccccc gagcacaaag gccgcggcta cgaggtggcc
     4261 aagggtcagt acctcatcat tgaggatgcg gagctcgaag cgatcgagat cgagagcacg
     4321 cacaccatcg agatcgacag atttgtgccg cactcgcaaa tcgatcagcg cttcttcgac
     4381 agcccttatt acgtcatgcc cagcgagccg gccgggcagg aagcctttgc ggtgattcgc
     4441 gaggccatgc gcggcaaggg ggtggtggcg ctcgggcggc ttgtgctctc gaagcgcgag
     4501 cgggggatcg cgcttgagcc atacggcaag gggcttctcg gcaccacgct gcgctacccc
     4561 tatgaggtgc gcaaggttga ggattacttc tgcgacctgc ccgacctcac cattgcgccg
     4621 gacatgctca cgctggccga gcatatcctc gacagcaagg ccagcgagtt tgaccccgcg
     4681 accttccggg accgctacga ggaggcactg ctggcgcatc tgaaggccaa gcaggccggt
     4741 gccgttccag agcgcaggaa gacctttgcc ccgccgcagc gggtggtcaa tctcatggag
     4801 gcattgcgcc gcagcgtcgc cgaggacact aaaggcgccg caccgcgcaa gggagcctcg
     4861 gcaaccccgg cgcgcaaacg ggcgtgagtg ccttagccca tcgcgccgca atgctgcggc
     4921 ttgccgacag cgagcccacg ccaatggcga agctgtcgta ccgcggcatc tcggcgacaa
     4981 ctcaggcggc gcgacgtctg aaaggcaccg ctcgtccaca cgcccgtccc ggcgatgagc
     5041 cgtccctcgt gcgtcatggg atgtcggtcg catgaacgga cccatcagca agctcgcgcg
     5101 ctgcgccatc tatacgcgca agtcgaccga gtacaatctc gagcttgcct tcaactcgct
     5161 cgatgcccaa cgcgaggcct gcgaggcata tatcaagagc caagcgcacg aaggttggcg
     5221 gctgatttcc ggccgctacg acgatggcgc attctccggc gcttcgctcg accgcccggc
     5281 gctgcagcag ctcttggccg atgtccgcgc cagcaagatc gacatcgtgc tggtttataa
     5341 ggtcgaccgg ctgacccgtt cgctggctga tttcgccaag ctcatcgagc tgttcgatac
     5401 ccacggcgtc tcgtttgtgt cggtgaccca atccttcaac accagctcca gcatggggcg
     5461 gctcacgctc aatgtgctgc tgtcatttgc ccagttcgaa cgcgagctga tcggggagcg
     5521 ggtgcgcgac aagatcgctg
//
LOCUS       DATLJT010000008         6784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2067998,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000008 DATLJT010000000
VERSION     DATLJT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067998"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VM910_00115"
     CDS             complement(<1..210)
                     /locus_tag="VM910_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HVI60990.1"
                     /translation="MTFVEDRGAAAQFKELTLPLLGEVYTVARYLLDSAADADDAVQE
                     CYLRAFRHFDTYHGPAIKPWLFTILR"
     gene            complement(197..331)
                     /locus_tag="VM910_00120"
                     /pseudo
     CDS             complement(197..331)
                     /locus_tag="VM910_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amicyanin"
     gene            complement(357..1259)
                     /locus_tag="VM910_00125"
     CDS             complement(357..1259)
                     /locus_tag="VM910_00125"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HVI60991.1"
                     /translation="MPKRRTDMSTLLELQMRLRRAVLGGDTGEVVAAIQGDGLDPAAR
                     VGIYRHHAFTTLGDALQTTFPVVCRLVDKRFFAYAAHEYLRQHPPHSRCLVEYGADFA
                     DFLARFTPCEKLPYLADVARFEWALNIAATVGEAAPLHAETLAAIPPGKAAYVALRLQ
                     PSLSYFASPWPIDTIWQANQADEVPPIDLARGGASLEIRRVGEAVSWRRLDLGTFAFR
                     TALADGLVLAAAMSAATFKDPAFDLTVALRQVFSEGLAVDFGRPGSTSRIRELATERQ
                     ERPNSSTRSHYATTNDMRCWSLQL"
     gene            complement(1222..2061)
                     /locus_tag="VM910_00130"
     CDS             complement(1222..2061)
                     /locus_tag="VM910_00130"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HVI60992.1"
                     /translation="MTRGRPIASGRVGIGLHLPHVSEIAAARPRLGFLEVHAENYMAE
                     TPALDRLLGLRRDYPVSLHGVALSLGSAERLDHCHLMRFKALIERVEPMLVSEHLAWS
                     AISGAYLNDLLPLPYTEASLDLFCRHVDEAQEGLGRRLLIENPSSYLRYQDSTIPEAE
                     FMTEVAHRTGCGILCDVNNVHVSAWNFGFDPLAYLDALPIEAIGEIHLAGHRVAEDVG
                     ILIDDHGARVAEPVWGLYAAALRRFGPVPTLIEWDTNLPTLEVLLNEARHAEEAHRYV
                     DAA"
     gene            complement(2089..2262)
                     /locus_tag="VM910_00135"
     CDS             complement(2089..2262)
                     /locus_tag="VM910_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HVI60993.1"
                     /translation="MKTLLLIADAGSVPTPQFAAEKHWCSYVSRQARDANSWIYVPVG
                     TCTKIAGGSLKKA"
     gene            3262..4290
                     /locus_tag="VM910_00140"
     CDS             3262..4290
                     /locus_tag="VM910_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI60994.1"
                     /translation="MTNQKPVNLALQGGGAHGAFAWGVLDKLLEDGRLTIDAMSATSA
                     GAMNAVAAAYGLSIGGPDGAREKLEEFWRRVSSAGQLYSPVRSLPWEKWLQGGAPLSD
                     FSPTFFVFQAMTHIFSPYHINPFNLNPLKEALAEVVDFSRLTKCNKAPRLFLSATNVR
                     TGKIKVFENDAISVDVVLASACLPYLFHAVEIDGEHYWDGGFMGNPAIFPLVYNCGSK
                     DVILVHINPIERKKLPMTAAEIFNRMHEISFNSSLMREMRAVAFVTKLIDDNVLDQRK
                     YRRMLIHSIRDDAAMVELGVASKLNPDWDFLCRLRDSGRTKAAEWLDCNFDEVGQSST
                     VDLAEVFL"
     gene            4346..5722
                     /locus_tag="VM910_00145"
     CDS             4346..5722
                     /locus_tag="VM910_00145"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI60995.1"
                     /translation="MTLFETLRNVLPRSLFTHLGEPPRAIEVPHRVAELIRAREEDSE
                     RLIGWVQLVMAATFAALYVIAPRPLDAGMSMLAPVPLALSGYAAFTAARLWFAHRGFL
                     PGWLLVLSMLVDTALLLGLIWAFHFQYEQPAAFSLKVPTFVYIFAFIALRALRFDHRY
                     VLSAGLFAAAGWALLVILAIQLSDGAGITRNFVAYITSNSILIGAEFDKIFTILMVTA
                     LLAIAVQRARSMLVTAMREEAAGREVRRFLSEGVAEAIARSETLIEAGHAVERDAAIL
                     MLDIRGFTRFSMTVPPKDVVQMLTSLHMRIIPIVRAHGGVVDKFLGDGVMATFGAVMP
                     SRTAAADALRALDAIMEEAGSWRQAPPAVGVAVPLEVNGAVSAGPVVFATLGGGDRLE
                     YTVIGEAVNLAAKLEKHNKAACTRALTSAATYELAVAQGYAARGERRPGSSVAGVSDS
                     IDLVVLKS"
     gene            6023..6205
                     /locus_tag="VM910_00150"
     CDS             6023..6205
                     /locus_tag="VM910_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HVI60996.1"
                     /translation="MGIRDGPTSFRSPWQNGYVERFIGSIRRECTDHLLVFNAEHLRR
                     ILAKYATYYNEVRTLQ"
BASE COUNT         1278 a         2006 c         2162 g         1338 t
ORIGIN      
        1 ccgcaggatc gtgaagagcc atggctttat cgccggtccg tgatacgtgt cgaaatgtcg
       61 gaaggcgcgc agatagcatt cctgcacggc atcgtcggcg tctgctgcgc tgtcaagcag
      121 atagcgggcg accgtgtaca cctcgccgag tagcggcaaa gtgagctcct tgaactgagc
      181 tgccgcgccg cgatcctcaa caaaagtaat cgtaaacgcc gggcgtcgcg aacgtgaacg
      241 agaatttgtg gtcggtgtcg agcaccttcg atttgaagac cttggtattc gaagcgagcg
      301 cattagggat gtcgtcccga ttcacccagg tgaagccacg gaggtagaac cgaaaatcag
      361 agctgcagcg accagcatct catatcgttg gtggtggcgt aatgcgacct tgttgaagaa
      421 ttcggacgct cttgccgctc ggttgcaagc tcacggattc gtgacgtcga ccccggcctc
      481 ccgaagtcga ctgccaaccc ttcggagaaa acctgccgaa gggccactgt caggtcgaag
      541 gcggggtcct tgaatgtcgc agccgacatc gccgcagcga ggacgaggcc gtcggcgagc
      601 gccgttcgga aagcgaaggt ccccagatcg agccggcgcc agctgaccgc ttcaccgacg
      661 cggcggatct ccaggctggc gccgcctctc gcgagatcga tgggcggcac ctcatcggct
      721 tggttggcct gccagatcgt gtcgatcggc cagggggagg cgaagtagct cagcgagggc
      781 tgcagacgga gcgcaacgta tgcggctttc cccggaggga tcgcagcgag cgtctccgcg
      841 tgcagcggcg ccgcctcccc aaccgtggcg gcgatattga gggcccattc gaagcgcgcg
      901 acatccgcca ggtaaggcaa tttctcacac ggggtgaagc gggcgaggaa gtcggcgaag
      961 tcggcgccgt actcgacaag gcagcgcgaa tgcggtggat gctggcgcag atattcgtgc
     1021 gcggcatagg cgaagaagcg tttgtcgacc agacggcaga ccacggggaa ggtggtctgc
     1081 aatgcatctc cgagggtcgt gaaggcgtgg tggcggtaaa tgccaactcg agccgccgga
     1141 tcgagcccgt cgccctggat ggcggcgacg acctctccgg tgtcaccacc gagaacggct
     1201 cggcggagcc gcatctgcag ctcaagcagc gtcgacatat cggtgcgcct cttcggcatg
     1261 acgcgcctcg ttcagcagga cctcaagcgt aggaaggttg gtgtcccatt cgatcagagt
     1321 gggcacgggg ccgaagcgcc ggagcgctgc ggcgtaaagc ccccagacag gctctgcgac
     1381 ccgggcaccg tggtcgtcga tcaggatacc cacgtcctcg gcaacgcgat ggccggcgag
     1441 gtggatctcg ccgatcgcct cgatcggcag tgcgtcgaga taggcaagcg gatcgaaccc
     1501 gaaattccac gcgctgacat ggacgttgtt gacgtcgcag aggatgccgc agccggttcg
     1561 atgggcgact tccgtcataa actccgcctc cggaatcgtg gaatcctggt agcggaggta
     1621 ggaggacggg ttctcgatca gcaaccgccg gccgagtccc tcctgcgcct catcgacgtg
     1681 ccggcagaac agatcgagac tcgcctctgt gtaaggcagc ggcagcaaat cgttgaggta
     1741 ggccccgctg atcgcgctcc acgccaggtg ctcagagacg agcatcggct cgacccgctc
     1801 gatcagcgcc ttgaagcgca tgagatggca atggtcgagc cgctcggcgc ttcccaggga
     1861 aagcgcgacg ccgtggaggc tcaccgggta atcccgccgc agcccgagga gccggtctag
     1921 cgccggcgtc tcggccatat agttctcggc gtgcacctca agaaagccga gccggggccg
     1981 agcggccgcg atctccgata cgtgcggcaa gtgcaggccg atgcctacac ggccgctcgc
     2041 gattggtcga cctctggtca tgatcgcggc ctcgcccggt cccttcggtc aggctttctt
     2101 gaggctgccg ccggcgatct tcgtgcaggt gccaacggga acgtagatcc aggaattcgc
     2161 gtcccgagcc tgcctggata catagctgca ccagtgcttc tcggccgcga attgcggtgt
     2221 tgggaccgac ccagcgtcgg cgatgagcaa tagggttttc atttcaggac tccatcgtgt
     2281 taggcgtgag aaccgagcga ctcggctgcg atgatcgagg gaacagcggc atcgctgttc
     2341 gctggcggtc ggcagattgg tcgcggtcac gcttgcagga aaagcaaagc tgatcgatgt
     2401 gatcttacac acgtcagtct ctcgctcttg aacgttgctc tgacgaagag ttcggaaagc
     2461 aggtcgttac ggttacaaca tcacgcggaa gtgaaaagat ggagctgctc ctttcgaccg
     2521 tggtgcaggc gtgctcaccg ccgccgcctg accgcgccgt aaacactatc gcctactagc
     2581 ttctggggcc gaaggccgaa gcggcgtttg atgcgatcaa tcatggtttc ggtgatggca
     2641 atctcgcggc acgattagcg cgcgtgccct cggcatcaac gatgatccca gccttgttgt
     2701 cgatgagata gtttgtatcg taagcaaaga acgggtcggt gcccttcctt gcaacccatg
     2761 ccgcctgtgg atcggtcagc gagacttctt tcggaggttt tcaggttcgg gctgcgggca
     2821 ggcagcacga gcggttcgac ttgacccgac gcaatgatcg ctcggaatga ctgggtgtac
     2881 ctgctgtcgc ggtcgtgcag cagatagcgg caatcccgca gcgcgccgcg caggacattg
     2941 ccgaccgtct gatcgcagac ctgatacccc agattggcca gcgccccacg atgcggtcat
     3001 agccccaatc atggttctcc tgggccatgc gggtgggggc gcttcatttt tgtgccgtta
     3061 ggtcaaacac cccaccgagt gaatagacgc cgagtccatt acgacgcaat ttgcgcaatt
     3121 ccggggcata aatagcgccc gcgaccaact cggttggtga cgaccaattg atcgagaagt
     3181 gatgctatag cgggcgcgca ggtgctactg catcgcgcag tcatgactcg ggtaatcggc
     3241 cgctctccag gatgtggacc gatgaccaat cagaaaccgg taaacttggc gttgcagggt
     3301 ggaggcgcgc atggcgcatt cgcctggggc gtgctcgata agctgctcga ggatggccgg
     3361 ctcacaatcg acgcgatgag cgcgacgagc gccggtgcca tgaacgctgt cgccgccgcc
     3421 tatgggctat cgatcggtgg gccggacggc gcgagagaga agcttgaaga gttctggcgg
     3481 cgcgtctcga gtgccggcca gctctacagt cctgtccgtt cgttgccgtg ggagaaatgg
     3541 ctgcagggtg gcgccccgct ctcggacttt tcgccaacgt tcttcgtctt tcaggccatg
     3601 acgcacattt tctcccccta tcacatcaac cccttcaacc taaatccgct gaaggaggcg
     3661 ctcgcagagg tcgtcgactt ttctcgactc acgaagtgca acaaggcgcc gcgactattc
     3721 ctttccgcca ccaatgtccg cacgggcaag atcaaggtgt tcgagaatga cgccatatcg
     3781 gtcgacgtgg tccttgcgtc tgcctgcctg ccctaccttt tccatgctgt cgaaatcgat
     3841 ggggagcact attgggacgg cgggttcatg ggcaatcccg caatctttcc actggtttac
     3901 aattgcggtt ccaaagacgt gatcctggtc cacatcaatc cgatcgagcg caagaagctg
     3961 cccatgacag cggcagaaat cttcaatcgc atgcacgaaa tcagctttaa ctcttcgctg
     4021 atgcgcgaga tgcgcgccgt cgcgttcgtt acgaagctta tcgacgacaa tgttctcgat
     4081 caacgcaaat accgccgcat gcttattcat tcgatccgcg atgacgcggc gatggtcgag
     4141 ctcggagtgg cgagcaaact caacccggat tgggacttcc tgtgtcgcct gcgcgacagc
     4201 gggcgcacca aggcggccga gtggctggac tgcaatttcg acgaggtcgg gcaatcgtcg
     4261 acagtcgatc tggccgaggt ctttctttag gccctactcc gaattggcga tgccgaattg
     4321 tgcggccatg ccgggaggtg aagtcgtgac cttgttcgaa acactgcgca acgtattgcc
     4381 gagaagcctt ttcacgcacc ttggtgagcc gccgagggcg atcgaggtgc cgcaccgcgt
     4441 tgcagagctc atacgcgccc gggaggagga cagcgagcgt ctgatcggct gggtgcagct
     4501 cgtgatggcg gcgacctttg cagcgcttta cgtgattgca ccacggccgc tcgatgccgg
     4561 gatgagcatg ctggcgccag ttccactggc gctcagcggc tatgcggcct tcacggccgc
     4621 gcggctgtgg ttcgcccacc gcggcttcct gcctggctgg ttgctcgtgc tgtcgatgct
     4681 cgtcgacacg gcgcttctgt tgggactcat ctgggcgttc cacttccagt acgaacagcc
     4741 ggccgcgttc tcgctcaagg tcccgacctt cgtctacatc ttcgcgttca tcgcgctccg
     4801 cgccttgcgg ttcgatcacc ggtacgtgct gtctgcggga ctgtttgccg cggccggctg
     4861 ggcgctgctc gtaatccttg cgattcaatt atccgacgga gctgggataa cccgcaattt
     4921 cgtcgcatac attaccagca acagcatttt gatcggcgcc gagttcgaca agatctttac
     4981 catcctgatg gtgacggccc tgctcgccat cgctgtccag cgagcccgat cgatgctggt
     5041 aactgcgatg cgtgaggagg cggcaggccg tgaggttcgc cggtttctgt ccgagggcgt
     5101 tgccgaggcc atcgcacgct ccgagacgct gatcgaggcc ggtcacgccg tcgagcgcga
     5161 cgccgcgatt ctaatgctcg atatccgcgg cttcacgcgc ttctcgatga cagtcccgcc
     5221 gaaggacgtg gtgcagatgc taaccagcct ccatatgcgg atcatcccca tcgtgcgtgc
     5281 acacggcggc gtggtggaca agtttcttgg cgatggtgtg atggcgacgt tcggcgcggt
     5341 catgccatcg aggaccgccg ccgcggacgc cctacgggct ctcgacgcga tcatggaaga
     5401 ggccgggagc tggcggcaag cgccgccggc tgtcggtgtc gccgtgccgc ttgaggtcaa
     5461 cggtgccgtg tctgccgggc ccgtcgtttt cgcgacgctc ggcggtggcg accgcctcga
     5521 gtacacggtg atcggcgaag cggtgaacct ggcggcgaaa ctcgagaagc acaataaggc
     5581 agcgtgtacg cgagcgttga cttcagcggc gacttacgag ctggcggtcg cccaaggcta
     5641 tgccgcgcgg ggcgagcgcc ggcccggctc gtcagtggcc ggggtatccg actctattga
     5701 tctggtggtg ctcaagtctt agacccggca tgtccgtccg tgcataaaga gcatagcagc
     5761 gcgaccatcg tcgctgacgt taagcgattc tgtcaccatt ccgcccgttg tcgcttccga
     5821 aaaccatccg attgactcgc ggtcagcagc gaatcagaac atgatcgccg catgattgca
     5881 atcggtctcc tattcgtccg catgctgtgc gactacttca agccgcgacc gcagctagaa
     5941 gccgagatcg tgatcctgcg gcatcagctc aacctcctac agcggcgagc gccacgccgg
     6001 cggcatttgc gttgggtcga tcgtggggat ccgggacgga cccacgtcat tccgttcccc
     6061 ttggcaaaac ggatatgttg aacgcttcat cggctcgatc cggcgcgagt gcacggacca
     6121 tttgctggtg ttcaatgccg agcaccttcg gcgaattctc gcgaaatacg cgacctatta
     6181 caatgaggtt cggaccctac aatgaggttc ggacccacgt ttcgctcggt aaggatgcgg
     6241 cccgtcgaac ggttcggtca cattgtcgcg tatccggtcc ttggcgggct acaccaccgc
     6301 tatgcccgaa tctaagtttt cggaagcgac aggacgtgaa ttgcgacaag cagcctaccg
     6361 caggagagcg tgcgctgtcg gtgcgttggg tcaggctgac gctgactaag gagagattcg
     6421 aaaggaatta aaacacgacg gtggccccag gtgcgggggt tgcctaacct ggggcctgcg
     6481 gcgtacatga caggacctgt tcgggtttcg aggatcgcca ttcggcattg cgaggaaagc
     6541 cgctgggttc ggagcaggat ggttctcgtt ccgactgtct ggctgggcgc ggttgtagtt
     6601 gtcgacggta accgggacag cagtgcctga cggcgcttgc gcgagcgcga cagagctcgt
     6661 tgccgccagc gacaaggcgg cgcagagttg agtgcggaaa tttgtcatca cagtggaatc
     6721 ccggggttta atgtaaaagc tgggtaccgt ctgattttgt tggcggagca actgggactc
     6781 cagt
//
LOCUS       DATLJT010000009         4801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2068101,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000009 DATLJT010000000
VERSION     DATLJT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068101"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..708)
                     /locus_tag="VM910_00155"
     CDS             complement(1..708)
                     /locus_tag="VM910_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI60997.1"
                     /translation="MLLEVRDLEVAYGDYQVVWGVSLSVAAGESVALLGPNGSGKSTV
                     VNTISGLLAAKAGEIRFDGASLKGRPTHVRASLGIAHVLERRRVFPHLTVLQNLTLGA
                     WHAAAKAARAPSLERMFTLFPRLSECKSQLARTLSGGEQQMLALGRGLMALPRLLIVD
                     EPFLGLAPRVVEQMKSVFEALQAEGIAILFIEQNVRLALSIAGRGYILESGRLAIEGD
                     GPTLINSPEVRRIFLGG"
     gene            complement(698..1426)
                     /locus_tag="VM910_00160"
     CDS             complement(698..1426)
                     /locus_tag="VM910_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI60998.1"
                     /translation="MSPLLDVQGLTKRYAGLTANAHITFDVNEGEIVGIIGPNGAGKS
                     TLFDLITGFQPPTAGRVFLDGHDITGLRPDRIGRLGLARTFQKLKPFVDLTVTENVMV
                     GALARTSDLKTARERALESLAFVDLLEKRNHFARELSTGQRKRLELARGLAMSPRLIL
                     MDEVTGGVDQRSIAGLMALVLELKQRGLTIVMIEHNMQVIMRLADRILALHAGRVIAF
                     GAPEEVRSDAAVVDAYLGTIDHAA"
     gene            complement(1423..2430)
                     /locus_tag="VM910_00165"
     CDS             complement(1423..2430)
                     /locus_tag="VM910_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI60999.1"
                     /translation="MLKSALAERLWPRSPLWVRAFGAAALALLLAYPFIFTTPFAHHL
                     MILILLYALMAQSWNVVAGLSGQISLGQAIFFGIGAYSSTVLFAKYGITPWAGLMVGM
                     LLSALAAVAIGVPTLRLRGHYFAIATLLIGSSVQIVFQRWDWVGAASGLYVPIERASP
                     WFYLQFHTSKVPYYYLALGAVAAGYLAVWKLRHSRLGFRLQALRDEPDAAASLGIAIA
                     RHKVMAFMISAAMMSVAGTFYAQYVLVLDPERLFSAEISIIVLLMTVLGGSGTLWGPA
                     LGAAILVPLSEYSRIWFGGTGRTIDLILYGILILLVCMWRPSGILSLLEERIRRRAGT
                     P"
     gene            complement(2454..3305)
                     /locus_tag="VM910_00170"
     CDS             complement(2454..3305)
                     /locus_tag="VM910_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61000.1"
                     /translation="MLQAVISGLLMGLIYALVAVGLTLIFGLMEIVNFAHGEFLMLAM
                     FTAFWFWALAGLDPVLSLPFVVIILALGGILAHFGIIRRLLKAPMLAQVCGTFGLGIA
                     LRAAAQFLWTPDFRTVTNPVIAGRVQIGSIFIGQPQLITGGVCLLSFAALWWFVTRTE
                     TGLGLQATAQDRQAAAVIGIPTERMFALGWAIGLGCVGIAGTMLSTFYYIFPEVGTNF
                     ALLAFVAVALGGFGSIAGSLLAGLLIGLVESLGGLLLDPSYKTLIVFALYLAVVIFRP
                     QGLFGRF"
     gene            3570..>4801
                     /locus_tag="VM910_00175"
     CDS             3570..>4801
                     /locus_tag="VM910_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61001.1"
                     /translation="MRSRIVTLACVAMLSGAASAQAQQEVKIGVLDPLSGPTAQIGID
                     AVAAIKTAAEIVNEGADLPLALARNKGLPGLGGAKVTLVVVDHQGKPEVGQSETERLI
                     TQEKVQALIGAYFSSVTAAASQAAERAGVPFINGDSTQPALTQRGLKYFFRTTPTDEN
                     FSELMFDFLKDFAAKSGQKFQSVSLFHEDTAYGTDSAKVQERLAGERGFRVLEKIAYK
                     AQTTSLTAEVQRLKAANADVLMPSSYTSDTFLLLRTAKDLDYNPKLIVAQNAGFTDPT
                     FISTMGKDAEGAITRSPYNADLESRIPLLPKINAIFKKHSNGRDLSDVPARNFTAAMT
                     LFDAINRAGSTDPEKIRTALAATNIPPDQLIVPYRGVRFATTGQNELVRAILMQVQKG
                     KYCTIYPFELASCQVLYPT"
BASE COUNT         1028 a         1536 c         1468 g          769 t
ORIGIN      
        1 tcatccaccg agaaaaatcc gtcgtacctc cgggctgttg attagagtgg gtccgtctcc
       61 ctcgatcgct aggcgcccgc tttccagaat atagccgcgg ccggcgatgg aaagcgcgag
      121 gcggacgttt tgttcgatga ataggatcgc gatgccttct gcctgaagcg cctcgaacac
      181 cgacttcatc tgctcgacaa cacgcggcgc gagcccgagg aacggctcat cgacgatgag
      241 cagccgcgga agcgccatga ggccacggcc gagcgcgagc atctgctgct cgccgccgga
      301 aagggtgcgc gcgagctggc ttttgcactc ggagagacgc gggaacagcg tgaacatccg
      361 ttccaagctt ggcgcacgcg ctgccttcgc tgccgcatgc cacgcgccca aggtcaaatt
      421 ctgcagcacg gtcaggtgcg ggaaaacacg tcgtcgctcg aggacgtgag caatgccgag
      481 acttgcccgc acgtgcgtcg gccggccctt gagactcgcg ccgtcgaagc gaatttcgcc
      541 ggccttggcc gcgagcagac cgctgatggt gttgaccacg gtgctcttgc cggacccgtt
      601 ggggccgagc aacgccacgc tttccccggc ggcaaccgag agcgacacgc cccaaaccac
      661 ctgatagtcg ccgtaggcga cttcgagatc gcggacctca agcagcatgg tcgatggtcc
      721 cgagataggc gtccacgacg gcggcgtcgc tgcgcacctc ttccggcgcg ccgaaggcga
      781 tgactcgccc cgcgtggagc gccaggatgc gatcggccag gcgcataatc acctgcatat
      841 tgtgctcgat catcacgatg gtgagtcccc gctgcttgag ctcgagcacg agcgccatca
      901 agccggcgat ggagcgctgg tcgacgccgc cggtcacctc gtccatgagg atgagccgcg
      961 ggctcatggc gagcccgcgc gcgagctcga gccgcttgcg ttgcccggtg gagagctcgc
     1021 gcgcaaaatg attgcgcttc tccagcaggt ccacgaaggc gagtgactcc agtgcccgct
     1081 cgcgcgcggt tttcaagtcc gacgtgcggg cgagcgctcc gaccatgaca ttttccgtga
     1141 cggtgagatc gacaaagggt ttgagcttct gaaacgtgcg ggcgagcccc aagcggccga
     1201 tgcggtcggg ccgcaggccg gtgatgtcgt ggccgtccag gaatacgcga ccggcggtgg
     1261 gcggctggaa accggtgatc aggtcgaaca gcgtcgactt gccggcgccg ttcggaccga
     1321 tgatgccgac gatctcgccc tcgttcacat caaaggtgat atgggcgttg gcggtcagcc
     1381 cggcgtagcg cttcgtcagg ccttggacgt cgagcagcgg gctcatggcg ttcccgcgcg
     1441 acggcgaatg cgttcttcca gcaggctgag aatgccgctc gggcgccaca tgcacacgag
     1501 caggatcaag atcccgtaaa gaatgagatc gatcgtgcga ccggtgccgc cgaaccagat
     1561 ccggctgtat tccgacaagg gcaccaggat cgctgctccc agcgccggtc cccacaacgt
     1621 gccgctcccg ccgagcacgg tcatgaggag aacaatgatc gaaatctccg ccgagaacag
     1681 ccgttccggg tccagaacga gcacatattg cgcataaaat gtgcccgcga ccgacatcat
     1741 cgcggcggag atcatgaagg ccatcacctt gtggcgtgcg atcgcgatgc cgaggcttgc
     1801 ggcggcatcc ggttcatcgc gcaatgcctg caggcgaaag ccgagccggc tgtggcgcag
     1861 cttccacacc gccaggtagc cggccgccac cgcacccagc gcgaggtaat agtacggcac
     1921 cttgctcgtg tggaactgca gatagaacca gggcgaggcg cgttcgatcg gtacgtaaag
     1981 cccggacgct gcgcccaccc agtcccatcg ttggaagacg atctgcacgc tcgagccgat
     2041 gagcagcgta gcgatcgcaa aatagtgtcc gcgcaggcgc aaggtcggaa cgccgatggc
     2101 aacggcagcc aacgccgaca gcagcatgcc taccatcaat cccgcccagg gcgtgatgcc
     2161 gtatttcgcg aagagaacgg tcgaggagta cgcgccgatt ccgaaaaaga tcgcctgccc
     2221 gaggctgatc tggcccgaga gtcccgccac cacgttccac gactgggcca tcaaggcata
     2281 caacaaaatg agaatcatca aatgatgggc aaacggtgtg gtgaagatga aaggataggc
     2341 gagcaacagc gcgagcgccg ccgcaccgaa tgcgcggacc cagagcggcg accgtggcca
     2401 aaggcgctcg gccagcgcac tcttgagcat gtccctccgg ctgctcgaca ttgctagaac
     2461 cggccgaaca ggccctgcgg cctgaaaatg accaccgcaa gatagagtgc gaacacgatc
     2521 aacgtcttgt aggacgggtc gagcaagagc ccgccgagcg actcgacaag tccgatcaag
     2581 aggccggcga gaagactgcc ggcgatgctg ccgaagccgc cgagcgccac ggcaacgaat
     2641 gccagcagtg cgaaattcgt gcccacctcg ggaaatatgt aatagaacgt cgagagcatc
     2701 gtaccggcga tcccgacaca gccgagtccg atcgcccatc ccaatgcgaa catgcgctcg
     2761 gtcggaattc cgatcacggc cgccgcctgg cgatcctgcg cggtcgcctg caggccgaga
     2821 cccgtttcgg tgcgcgtgac gaaccaccac agcgcggcga acgacaacag gcagacgccg
     2881 ccagtgatca gctgcggctg gccgatgaag atggagccga tttgcacgcg cccggcaatg
     2941 actggattgg tgacggtgcg aaagtcgggc gtccagagga attgcgcggc ggcacgcagc
     3001 gcgataccga ggccgaacgt gccgcagacc tgggcgagca tgggtgcctt caacagccgc
     3061 cggatgatgc cgaagtgtgc caatattccg ccaagggcga ggataatgac cacgaacggc
     3121 agcgacagca ccggatcgag gccggcaaga gcccagaacc agaatgccgt gaacatggcg
     3181 agcatgagga actcaccatg cgcgaaattg acaatttcca tgaggccgaa aatcagcgtc
     3241 aggccgaccg ccacaagcgc gtagatcaac cccatcagca gcccactgat gacggcttgc
     3301 aggatgagct ctctagacct cgatgcgcgc gcccctcaaa acagctgaac cgattgacga
     3361 gtacatgcaa aaagcgctcc aatcagacta gcgaccccgg catttgtgag ccttggtcgc
     3421 acctgcgatg gcgagctttg gaagcagacg cactcagcaa ctctatcatt gtcgtgcggt
     3481 tttgcattcg aacccctgcc ccagttcgcc gcccccgcga taggaacttc agctaatcgt
     3541 gcgaatatga aggcgaacgg agcaccgcca tgagatccag aatcgtcact ctcgcgtgcg
     3601 tcgcaatgtt gagcggcgca gcatcggcgc aggcacagca agaggtcaag attggcgtgc
     3661 tcgatccgct gtcgggcccg accgcgcaga tcggcatcga cgccgtcgcc gcgatcaaga
     3721 cggccgccga gatcgtcaac gagggcgccg atctcccgtt ggctctcgcc aggaataagg
     3781 gcttgccggg tctcggcggc gccaaagtca cccttgtcgt ggtcgatcat cagggcaagc
     3841 cggaggtggg ccaaagcgaa accgagcgtc tcatcacgca ggagaaggtc caagccctca
     3901 tcggcgccta cttctcctcg gtgaccgccg cggcaagcca agccgccgag cgcgcaggag
     3961 tgcccttcat caatggagat tccacccagc cggcgctgac gcagcgcgga ctgaagtatt
     4021 tcttccgcac gactccgacg gacgagaact tcagcgagct gatgttcgac ttcttgaagg
     4081 atttcgcggc caagtccggc cagaaattcc aatcggtgtc tctcttccac gaggataccg
     4141 cctacggcac cgacagcgca aaagtccagg aacggcttgc cggggagcgc ggttttcgcg
     4201 tgctcgaaaa aattgcctat aaggcccaga cgacgtcgct caccgcggag gtgcagcggc
     4261 tcaaggccgc caacgccgac gttttgatgc cgtcgtccta tacatcggac acgtttctgc
     4321 tcctgcgtac cgccaaggat ctggactaca atccaaagct catcgtggcg cagaacgccg
     4381 ggttcactga cccgaccttc atcagcacca tgggcaagga cgcggagggg gcaatcaccc
     4441 gctctccgta caacgctgac ctcgaaagcc gcattccgct tcttcccaag atcaacgcca
     4501 tcttcaaaaa gcactcgaac ggacgtgatc tttcggacgt tccagctcgc aactttaccg
     4561 ccgccatgac cctgttcgac gcgatcaacc gcgccggctc caccgatccc gagaagattc
     4621 gcaccgcact tgcggcaacc aacattcccc ccgaccagct gatcgtgccc taccgcggcg
     4681 tcaggttcgc aacgaccggc cagaacgagc tggtgcgggc gatcttgatg caggtgcaga
     4741 agggcaaata ctgcacgatc tatccgttcg agcttgcgtc gtgtcaggtc ctttatccga
     4801 c
//
LOCUS       DATLJT010000010         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2987098,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000010 DATLJT010000000
VERSION     DATLJT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987098"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VM910_00180"
     CDS             <1..524
                     /locus_tag="VM910_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HVI61002.1"
                     /translation="QLNDRRVETVPVSRLIAYAGNARTHSRSQIRQIAGSIKCFGFVN
                     PVLADDADQIIAGHGRVEAAKLLGIEAVPVLRVTHLTGAEKRAYVLADNRLAELAGWD
                     RAILAIELQGLLDVGFEVEATGFSIPDIDLCCATIWMRTTAIEFEAAAGSAGMIVSII
                     EVRKLFLLPLDCR"
     gene            complement(555..1364)
                     /gene="istB"
                     /locus_tag="VM910_00185"
     CDS             complement(555..1364)
                     /gene="istB"
                     /locus_tag="VM910_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI61003.1"
                     /translation="MKTTDPVDAARIELLLAELRLPAVKAMWAKFAAQSDKEGWPAAR
                     FLAVLAEHEIADRDRRRIERHLAEARLPVGKTLASFDFDAVPMVSKAQVMALTSGDSW
                     IENGANLLLFGPPGAGKSHLAAAIGLALVENGRRVLFTRTSDLVQRLQVARRELALES
                     ALAKLDKYHLLILDDIAYISKDQAETSVLFELIGSRYERRSMLITANQPFGAWSKVFP
                     DQAMTLAAIDRLVHHATILEMNVESYRRRAALDRKRGPGRPPTHATIKENA"
     gene            complement(1361..2857)
                     /gene="istA"
                     /locus_tag="VM910_00190"
     CDS             complement(1361..2857)
                     /gene="istA"
                     /locus_tag="VM910_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI61004.1"
                     /translation="MKSRQNNVPAIAAAKAGFSTATAYRIEADPRLPSQKQTPRGRRR
                     PDPLAGVWDSEIVPMLNAAPGIRPVAIFEEICRRHPEIVTGVRRTLERRIGRWRALYG
                     PNRDVMFRQEHPPGRMGLSDFTAMAALGITIAGEPFDHRLYHFRLAFSGFEHAHVVLG
                     GESFVALAEGLQNALWALGGVPEQHRSDSLSAAFRNLDRRTQEDLTRRYEELCAHYGM
                     TPSRNNRGLAHENGAIESPHGHLKKALADELLLRGSRDFEDLTAYRRFIDEVIGRRNA
                     RNRKRIEIERAALRPLPERRTTDYEEARVLVTSSGGFILRRVFYSVPSRLIGHRLNVR
                     LYDDRLECFLGATQLLTLRRGRPPQGSGKHGHVVDYHHVIHALRRKPMALLNLVYRDQ
                     LFPHRAYARAFEALLARKSEKQACRTMVGLLALAHDRACEVELANAIDGDLDAGDLPD
                     LDRLRDRFRPDRDAIPEVAVAFVPLSTYDELAAVHQSAAVLALDGGLA"
BASE COUNT          571 a          961 c         1051 g          591 t
ORIGIN      
        1 cccagctcaa tgatcgacgg gttgagaccg ttccggtaag ccgcctgatc gcgtatgcgg
       61 gcaacgccag gacgcattcg aggagtcaaa tccgccagat cgccggcagc ataaagtgct
      121 tcggttttgt caacccagtg ctggccgacg atgccgacca gatcatcgct gggcatggcc
      181 gggtggaagc cgccaagctg ctgggcattg aggcggtgcc tgtactgcgg gtcacgcatc
      241 tcaccggtgc tgagaagcgc gcctatgtgc tggccgacaa ccggcttgcc gagctcgccg
      301 gctgggaccg cgcaattctt gccatcgaac tgcagggcct cctggatgtc gggttcgagg
      361 tcgaggcgac gggcttttcc attcctgaca tagacctctg ttgcgcgaca atctggatga
      421 gaacgacggc aatcgagttt gaagcggcag ccggtagcgc cggcatgatc gtgtcgatta
      481 ttgaggtccg caagcttttt ttgctgccgc ttgattgtcg ctgagtgtca gagttgattg
      541 tcgcggagcg acaatcaggc attttccttg attgtcgcgt gtgttggcgg tcgtccggga
      601 ccacgtttac ggtcgagcgc agcgcggcgt cgatagcttt cgacgttcat ctccaaaatg
      661 gtggcgtgat ggacgaggcg atcgatggcg gcgagcgtca tggcttggtc ggggaagacc
      721 ttgctccatg ccccgaatgg ctgattggca gtgatcagca tcgagcgtcg ctcgtagcgg
      781 ctgccgatca gctcgaacag cacgctggtt tcggcttgat ccttgctgat ataggcgatg
      841 tcgtcgagga tcagcaggtg atatttgtcg agcttggcga gcgcgctttc gagagcgagt
      901 tcgcggcgcg cgacttgcag gcgctgaact aggtcgctgg tgcgcgtgaa cagcacccgc
      961 cgtccgtttt cgacaagggc gaggccgatg gcggcggcaa gatgactctt gccggcgccg
     1021 ggcgggccga acaacaaaag attggcgccg ttttcgatcc aactgtcgcc ggaggtcagc
     1081 gccatcacct gagctttgct gaccattggc acagcatcga agtcgaagct tgcgagggtt
     1141 ttgccgaccg gcagccgcgc ttcggcgagg tggcgctcga tgcggcggcg gtcacggtcg
     1201 gcaatttcgt gttcagccag cacggccagg aagcgggcag cgggccagcc ttccttgtcg
     1261 gattgcgcag caaacttggc ccacatggct ttaacggccg gcaagcgcag ctcggcaagc
     1321 aggagttcga tgcgggcggc atcgacagga tcggtcgtct tcatgccaaa cctccatcga
     1381 gcgcgagaac ggccgccgac tggtgaacag cggcgagttc gtcgtaggtg gagagtggca
     1441 cgaaggcgac ggcgacctcc ggtatcgcgt cccgatccgg tcggaagcgg tctcgcagcc
     1501 gatcgagatc gggcagatcg ccggcgtcaa gatcgccatc gatggcgttg gcgagttcga
     1561 cctcacaagc gcgatcatgc gcgagtgcca ggagaccgac catggtgcgg caggcctgct
     1621 tttcgctttt tctcgccagc agcgcctcga aggcgcgggc ataagcgcga tgtgggaaca
     1681 actgatcgcg atagaccaga ttgaggagcg ccatcggctt cctgcgcagc gcgtggatga
     1741 cgtgatggta gtcgaccaca tggccatgct tgccgctccc ctgcggcggt cggccacgcc
     1801 gcaaggtcaa cagctgcgtc gcgccgagaa agcattcgag ccggtcatca tagaggcgga
     1861 cgttgaggcg gtggccgatc aggcgcgagg gaacgctata gaacacgcgc cgcagaatga
     1921 agccgccact cgatgtaacg agcacgcgcg cttcctcgta gtcggtggtt cgccgctcgg
     1981 ggagaggccg cagcgcggcg cgctcgatct cgatgcgctt gcggttcctg gcattgcgcc
     2041 gaccgatcac ttcatcgatg aagcgacgat aggcggtgag gtcctcgaag tcgcgtgagc
     2101 cgcgtaacag cagctcgtca gcaagtgctt tcttgagatg gccatgggga ctctcgatcg
     2161 cgccgttctc gtgcgcaagg ccgcggttgt tgcgcgacgg cgtcatgccg taatgagcac
     2221 acagctcctc gtaacgtcgt gtcaggtcct cctgggtccg acgatcaagg ttgcggaacg
     2281 ctgccgacaa gctgtcgctg cggtgttgtt ccggcactcc gcccaacgcc cacagcgcat
     2341 tctgcaggcc ttccgccagc gccacaaagc tctcgccacc gagcaccaca tgggcatgct
     2401 cgaagcccga gaacgccagt cggaagtgat agagccggtg atcgaacggc tcgcccgcga
     2461 tggtgatgcc aagcgctgcc atcgcggtga agtcggacag gcccatccgt cccggcgggt
     2521 gctcctgacg gaacatcaca tcccggttag ggccgtagag cgcgcgccat ctcccaatgc
     2581 ggcgctccaa ggtccggcgc accccggtga cgatctccgg gtggcgacgg cagatctcct
     2641 cgaagatcgc caccggccgg atgccaggag cagcattgag catcggcacg atctcgctgt
     2701 cccacacgcc agcgagcgga tcgggacgac ggcgcccacg gggcgtttgc ttctgcgaag
     2761 gcagccgcgg atcagcctcg atccgatagg cagtggcagt ggaaaacccg gccttcgcgg
     2821 ccgcgatggc agggacgttg ttctgacggg acttcatgta caacctcgtc tggcagtcag
     2881 tgatgtgtcg gccaggcaag ctgctgatcc ttctggtgac gaagaatcag atgctgcgcc
     2941 aaaccgctcc gaccgccaga cgcgttcgcc cgcctgggga aaacgcgccg ccggggatgg
     3001 gctgacttcg gtcgggctac gccctccctc cgtcacccca tccccggcgc ctttctcatc
     3061 ctgattgtcg ctcggttctc accttgattg tcgcgcggca cgacgtgacg gtgcgccggc
     3121 tgggcgccag aatgaccccg gcgatcagca ccagcacgtg cgaccaggta gggc
//
LOCUS       DATLJT010000011         3277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2527683,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000011 DATLJT010000000
VERSION     DATLJT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527683"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VM910_00195"
     CDS             <1..744
                     /locus_tag="VM910_00195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVI61005.1"
                     /translation="IDTLVGFFWIDEKPTGSKDPYALRRAALGTIRTVLSNDIRLALR
                     RKAIPAAMALFGHSKSASANRHIDKMSLETDLLAFFADRLKVQLREQGARHDLVDAVF
                     ALEGQDDLLMIVRRVEALAAFLDTDDGKNLLAGTKRANNILAIEEKRDKRRFDGAPNP
                     ALYSLAEEKALADAIDLVKVEASVAVMNEDFATAMGAMAKLRPAVDAFFDHVKVNDDD
                     PKIRENRLKLLNVIRSATRAVADFSRIEG"
     gene            746..1342
                     /locus_tag="VM910_00200"
     CDS             746..1342
                     /locus_tag="VM910_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI61006.1"
                     /translation="MDRQTRDAYDRGASAFARDWHEQPAPVDLQSTVARFFIKGGRTC
                     DIGCGSGREVAWLNANGFLAEGFDASDGLLAEARRRYPDLKFAHAELPELSGIAANTF
                     DNVLCETVIMHLDRALIAPSVHRLLDIAKPSGILYLSWRVTEGADLRDANGRLYAAFD
                     AALVREACANATLLLDEEVVSASSGKRVHRLVARKRDT"
     gene            complement(1470..1829)
                     /locus_tag="VM910_00205"
     CDS             complement(1470..1829)
                     /locus_tag="VM910_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HVI61007.1"
                     /translation="MRNRILAIAAIAAAIAAPAVAEAQSTMGVVRGGPVIVNEDAAGI
                     TVENVPAFREYVVRERVPEYTIPDRVIVGTVLPETGVTYYDVPQSFAVTPYRFTVVNG
                     RTVLVEPRSRRIVQVID"
     gene            2058..2213
                     /locus_tag="VM910_00210"
     CDS             2058..2213
                     /locus_tag="VM910_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HVI61008.1"
                     /translation="MTLTVAHISPIMSLIAGVLILIMPRLLNFIVALFLILNGLLGLG
                     LLKWLHL"
     gene            2318..>3277
                     /locus_tag="VM910_00215"
     CDS             2318..>3277
                     /locus_tag="VM910_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HVI61009.1"
                     /translation="MAKAAARPKKIVAKSKSSEATRAKPAPVSIARKALKKSTAKPVA
                     KAPGRSATAEAAKTGKWVYTFGDGKAEGQARLRDLLGGKGANLAEMANLGLPVPPGFT
                     IPTSVCTYFYAHDKTYPKELKAQVEKALDHVGKLTGKVFGDPKNPLLVSVRSGARASM
                     PGMMDTVLNLGLNDQTVEALAEMSGDRRFAYDSYRRFITMYSDVVLGFEHHHFEDILD
                     TFKDSQGYSLDTDLTADDWVELVGKYKEAVARETGKEFPQDPHDQLWGAIGAVFSSWM
                     NARAVTYRKLHDIPESWGTAVNVQAMVFGNMGETSATGVAFTRN"
BASE COUNT          661 a         1035 c          982 g          599 t
ORIGIN      
        1 atcgacacgc tggtcgggtt cttttggatc gatgagaaac cgacgggttc gaaggatcct
       61 tacgcgcttc gccgtgcagc gctcggcact atcagaaccg tcttgtcgaa tgatattcgg
      121 ctcgcgctgc gtaggaaagc aatcccggct gccatggcgc ttttcgggca ttcgaaatcc
      181 gccagcgcca atcggcatat cgataagatg tcgttggaga ccgacctcct cgcgttcttc
      241 gccgaccgac tgaaggtcca gcttcgcgag cagggtgcgc ggcacgatct cgtcgatgcc
      301 gtttttgcgc tggaaggcca ggacgacctc ttgatgatcg tgcgccgcgt ggaggcactt
      361 gcggcattcc tcgacaccga tgacggcaag aacctgctcg cgggcaccaa gcgcgcgaac
      421 aatattcttg cgatcgagga gaagcgggac aagcgcagat ttgatggcgc accgaatccg
      481 gcgctttata gcctcgcgga agaaaaggcg cttgccgacg cgatcgacct ggtgaaggtc
      541 gaggccagcg ttgccgtcat gaatgaggac ttcgccaccg ccatgggcgc aatggcaaaa
      601 ctgcgaccgg cggtggatgc gttcttcgat cacgtcaagg tcaacgacga cgatccgaaa
      661 atccgcgaga accgcctgaa actcctgaac gtgatccgca gcgcgacgcg cgcggtcgcg
      721 gacttttccc gaatcgaagg ctgagatgga tcgccagacg cgcgacgcgt atgatcgtgg
      781 cgcatcggcg tttgccaggg actggcacga gcaacccgcg ccggtcgacc tgcaatcaac
      841 tgttgcgcgc ttcttcatta aaggcggcag gacctgcgat atcggctgcg gcagcgggcg
      901 cgaggtcgcc tggctcaatg ccaacggatt tctggcggaa ggttttgacg cctccgacgg
      961 tctcctggcg gaagcgcgcc gccgctatcc cgacctcaaa tttgcgcacg ccgaattgcc
     1021 ggagctttcc ggcatcgccg caaatacgtt tgataacgta ttgtgcgaaa ccgtgatcat
     1081 gcacctcgac cgcgcgctga tcgcgccatc cgtgcaccgc ctgctcgaca tcgcaaagcc
     1141 ttcgggcatt ctttatctga gctggcgcgt caccgagggc gcggacttgc gcgacgcaaa
     1201 tggccggctc tatgccgcct tcgacgcggc gctggtacgt gaggcgtgtg cgaacgcgac
     1261 cttgctgctc gacgaagagg tcgtgagcgc gtcctccggc aaaagggtcc accgtctggt
     1321 cgcgcgcaag cgagatacgt gatgtcatct ccacgaggga gagacgcaat cacagcaaaa
     1381 aagccccacc cggcgggggg gaccgggcgg ggcctcaatg tccggttcgc cgctgcggtt
     1441 cgccggacaa tcaggtcatg cgctgagcgt cagtcgatca cctgcacgat gcgccgcgag
     1501 cgcggctcaa ccagcaccgt gcggccattc acgaccgtga agcgataggg tgtcaccgca
     1561 aaactctgcg gcacgtcgta ataggtcacg ccggtttccg gcagtaccgt tccgacgatc
     1621 acgcgatccg gaatggtgta ctccggcacg cgctcgcgaa cgacatattc gcgaaatgcc
     1681 ggcacattct cgactgtgat gcccgcggcg tcctcattga cgatcaccgg gccaccgcgc
     1741 accacgccca tggtgctctg agcctcggct accgcaggag cagcgattgc agccgcaatc
     1801 gcggcgatgg caagaatcct gttccgcatg gatacctccg tgagaatagg caagctcccg
     1861 gcccgtcccg catcccaatg catcgcgtgc acgatcgttc ccgggaacag ccgaacccac
     1921 cggcggcaat ttcaggcaat ttttgtgatg cggggaacct tgcgccgtcc cgcggcgtct
     1981 cctattcgca aagccccgac gatagaatgc gggcccgaat cgaccttccc cctatcgcta
     2041 tcgcggagag ctttcagatg accctgacgg tcgcccacat ctcgccgatc atgtcgctca
     2101 ttgcgggcgt tttgatcctg atcatgccgc gacttctcaa cttcatcgta gcgttgttct
     2161 tgattctcaa cggcctgctg ggtctcggat tgctgaaatg gctgcacctc taattcccaa
     2221 aaactggaac ccgcctcctt gcgcgggctt gcccaaaatg gtgtaaggcg cccaatccat
     2281 tacaacccct caccctccca gcggattgat ccaagccatg gccaaagccg ccgcaagacc
     2341 taaaaaaatt gttgcgaaat caaagtcttc cgaggccacc cgggccaagc ccgcgccggt
     2401 gtcgatcgcc cgcaaggcgc tgaagaagag cacggccaag ccggtcgcga aagcgccagg
     2461 ccgatccgcg acagcggagg ccgcaaagac cggcaagtgg gtgtacacct tcggcgacgg
     2521 caaggccgag ggccaggcac ggctgcgcga tctgttgggc ggcaagggcg caaatctcgc
     2581 ggaaatggcc aatctcggac tgccggtgcc gcccggcttc acgatcccga cctcggtgtg
     2641 cacttatttc tacgcccacg acaaaaccta tccgaaggaa ttgaaggcgc aggtcgaaaa
     2701 ggcgctcgac catgtcggca agctgaccgg caaggtcttc ggcgatccca aaaatccgct
     2761 gcttgtctcg gtgcgttcgg gagcgcgcgc gtcgatgccg ggcatgatgg acaccgtgct
     2821 caatctcggc ctcaacgatc agaccgtgga agcgctggcc gagatgtcgg gcgatcgccg
     2881 cttcgcctat gacagctatc gccgcttcat caccatgtat tcggatgtcg tgctcggctt
     2941 cgagcatcat catttcgagg acatcctcga caccttcaag gacagccagg gctattcgct
     3001 cgataccgac ctgactgccg acgactgggt cgaactggtc ggcaagtaca aggaagcggt
     3061 cgcgcgcgag accggcaagg aatttccgca agacccgcat gaccagttgt ggggcgcgat
     3121 cggcgcggta ttttcatcct ggatgaacgc gcgcgcggtg acctatcgca agctgcacga
     3181 catcccggaa tcctggggca ccgcggtcaa cgtgcaggcc atggtgttcg gcaacatggg
     3241 cgagacgtcg gcgaccggcg tcgccttcac ccgcaac
//
LOCUS       DATLJT010000012         9507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1608699,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000012 DATLJT010000000
VERSION     DATLJT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608699"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..165)
                     /locus_tag="VM910_00220"
     CDS             complement(40..165)
                     /locus_tag="VM910_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61010.1"
                     /translation="MTAQIINLAAYRKAKEAEEVRKVVNDYFAAHSQIMAWWLSK"
     gene            complement(197..574)
                     /locus_tag="VM910_00225"
     CDS             complement(197..574)
                     /locus_tag="VM910_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI61011.1"
                     /translation="MAARAVTRLYDDTLRSVGLRATQLAVLVAVGGGDVVSITALAKF
                     MGMDRSTLTRNLTPLERDGLIRVGNEGWRRSRTVEITKKGRSRLREALPLWEKAQETL
                     RRKLGLRRWAVIRADLDDLIRAA"
     gene            complement(652..1020)
                     /locus_tag="VM910_00230"
     CDS             complement(652..1020)
                     /locus_tag="VM910_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61012.1"
                     /translation="MRLRSLPCFAGGRAVDALRSITVAQHLFGIQNIIVVHHSHCGAT
                     SFTADGIIKAYEYEHHVDISNLYDRSNICITDYEASLKHDTALVRSHDGTPKNVDIFG
                     YFYDIDTSALTEVVRDIHRD"
     gene            complement(1384..5961)
                     /locus_tag="VM910_00235"
     CDS             complement(1384..5961)
                     /locus_tag="VM910_00235"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61013.1"
                     /translation="MPNGKPYPSGTAYELLTALEASKAGGVPDVYVFRKTADATLPMA
                     DVERRRQAQTQLDALEAFWSEWFRSEKGEFKAAFQNFLSTDVFEHQVELLLRQWLETR
                     GFLGPRIAWPKEKGSPFRGLAPFEAEHAAVFFGRDRVNDEARRRFVGAAERGCPFLLI
                     VGASGSGKSSLARAGLIPRLTTPGVVASVDLWRVARMKPGEGQAGPLMALAMALLVAD
                     ALPELVHGDYPSAASLADNLRRGGTASVQPITSALARVAEDAQRERHADQPPKPVLVL
                     LVDQFEELFAQRVSDAERVAFAESLRQLIATGRVWMVATLRADLYELLLKQPALKALK
                     ETGASLDLGPPGPAELAEIVHAPAAAAGLVFETDTEKGELGERLLADAKTADSLPLLQ
                     FTLRQLYERREEIAGEARLTHAAYDQLGGLAGAIAAEAERAVSSLPSQAVATLPQLLR
                     RLAEPARDGKTLTLREVSHADVASQASEATLVDALLMARILIARTDAEGRPTVRLAHD
                     AVLASWPRASEAAQASREFYRVRAEVEDALRLWQEHGRPKDRLIQPGVPLAEAEKLVE
                     DFGAELPAEFTSYVKTSRNRARVRQRLVAAAAIFFFILAVAATATGIWAYRSQQEAVQ
                     AEARAVAERDRATQNFKLAKRTADSLVVDIARGLRNVLGMPADTVRKILEAAKATFEQ
                     LAASAPNDLDLQYSQSTMLEEFGKTYQTLGDLDAALKAYRDSVTIFERLIAADGSNMQ
                     WQHDLSISSIEVGDVLRAQGKLEEALKAYRDSLAIAERLAAANRNNTQWQRDLTASYD
                     RVGDALRAQGRLDEALKTFRDSLTIRERLTATDRSNTQWQRDLSVSYIKVGDVLKAQG
                     KLEDALKTFRESLAIAERLAATDRSNTQWQRDLAAFYATVGHVLKAQGKLGEALKTYR
                     DSLAIGERLTAADRSNTQWQRDLSSAYTDVGDVLVAQGKLEEALKAFRDSLAISERLA
                     AVDRSNMQWQSDVSASYVRIGDVLKAQGKLEEALKTFRESVAIAERLAATDRSNTQRQ
                     SSLAFSYSRVGDALVAQGKLEEALKTYRDSLVIRERLAATDPSDTQWQSDLATAFNTI
                     GYVLVQQGKLEETLKTYRDSLVIRERLAATDRSNTQWQRDLAWSYMNLGDVLVVQGRL
                     EEALKVYGDSLAVAERLAAADPSNTQWQSGLTWSYVKLGDVLKAQGKLEEALKPFRDS
                     LTIAERLAAADPSNTQWQSSLAVAYNRVGDALTAQGKLEEALKPFRDSLAIAERLAAA
                     DPSNAQWQSNLAVAYNRVGDVLVAQGKLEEARKVYRDSLAIAERLADVDRSNTQWQRD
                     VSFASNRVGDVLKAQGKLDEALKTFRDSLAIAKRLADVDRSNTQWQSDLSFAYNRVGD
                     VLVAQGKLEEALKVHRDSLAIAERLAAADRSNTQWQQNLQGSISSIGDLANELVLARD
                     FTRALEASDLVISIAPDQIWLYTNRAHALMFLARVDEARALYLQYRGEKNVAADKPWE
                     TVVLEDFAELRKAGLTHPLMDEIEKRFAAGG"
     gene            6363..7028
                     /locus_tag="VM910_00240"
     CDS             6363..7028
                     /locus_tag="VM910_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI61014.1"
                     /translation="MPAHQGKFIAYFRVSTDRQGKSGLGLEAQRKSVLDYLDGGRWNL
                     VGEFTEVESGKHNDRPELKSALAACKKQKAKLVIAKLDRLSRNLAFIATLMDSGVEFI
                     AVDNPHANKLTIHILAAVAQHEREIISARTSAALKAAKARGKRLGNPKLPEARRRAAL
                     AKKEKADRYSANVLPVIREIQRSGIKSLRGIARALAARGIRTARGGAWTPVQVTAILR
                     RAG"
     gene            7517..7822
                     /locus_tag="VM910_00245"
     CDS             7517..7822
                     /locus_tag="VM910_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HVI61015.1"
                     /translation="MSTKRPTLNRLTAYAWYLGSAFWRERREHILQRANYTCEKCGRR
                     PATEVHHLTYVRVFQELPADLVALCKQCHAEIHWQQPANDNQLQCSFDPPTALEEES"
     gene            7881..8375
                     /locus_tag="VM910_00250"
     CDS             7881..8375
                     /locus_tag="VM910_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVI61016.1"
                     /translation="MISSFFTTDQAAERLRKSRKWLQEWLCEHPISWSGMPYYMAIGR
                     TKLFTDENIRWIARAIDDQEAERKERNEAAKNDDGFIYFIEAGDFIKIGHTRSPVGRG
                     HKMRTDNPFDLKLLHIEDGTFKREKLYHRHFAAIRVRGEWFHKTPDLLAFIEQRKRIV
                     AGEP"
     gene            complement(8701..>9507)
                     /locus_tag="VM910_00255"
     CDS             complement(8701..>9507)
                     /locus_tag="VM910_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI61017.1"
                     /translation="CNMRCAFCQNGDISTDKDNGEETDPRTLAAMAWTLRREGCHNIN
                     WVGGEVVIHLHAIVDAIALLGRDFTPTPAELKRARKTKADRFLWFDEVPGAATYEGKF
                     NAPMLWNSNFFMTLESMKILRVLTDVWLPDFKFGPGRCAMTLAKTPWYWETVTRNIAL
                     LADWGEDFSIRHLVMPNHVECCTYPVLEWIAERTPAAPVSVMAQFRPDNFCDPASAKY
                     RDKYAEIARPPTRTELDDSWRHARELGLKFETATFEGRNGLGLTSMLELW"
BASE COUNT         1805 a         2954 c         2849 g         1899 t
ORIGIN      
        1 gtgccaaaag cagacatcgg ccccgcacct gtagctggcc tacttggata accaccacgc
       61 cattatttga ctgtgcgcag cgaagtaatc gttgaccacc ttacgaactt cctctgcttc
      121 tttcgcctta cggtacgccg caagattgat gatctgcgcc gtcatgacag tctcccgttt
      181 agggatagtt agattcttac gcggcgcgga tgagatcgtc caagtcggcc cggataacgg
      241 cccatctccg aaggcccagt tttcgcctca gtgtctcctg cgccttctcc cagagcggca
      301 atgcctcgcg cagtcgcgag cgtcctttct ttgtgatttc aactgtgcga ctacggcgcc
      361 agccctcgtt gccgacccga ataaggccat ctctttcaag cggagttagg tttcgcgtca
      421 atgtcgaccg atccatgccc atgaatttgg cgagtgcggt aatcgaaacg acatcaccgc
      481 ctccgacggc gaccaagacg gcaagctgcg tggcgcgaag cccgaccgat cgcaacgtgt
      541 cgtcatacaa acgggtcacc gcacgggctg ccattcgcgt cctatggcac acgcagttct
      601 cgacaacctc tcgctccgta accatctagg ccctcaatca tgcggccggg atcaatcccg
      661 gtggatgtct ctcaccacct cggtgagcgc gctggtatcg atatcgtaga agtagccgaa
      721 gatatccacg ttctttggcg tgccgtcatg cgatcgcact aaagccgtat cgtgcttcaa
      781 cgatgcttca taatcggtga tgcagatgtt gcttcgatcg tagagattcg agatatcgac
      841 gtggtgttcg tactcgtagg ccttgatgat cccatcagcc gtaaagcttg tggcgccgca
      901 gtgcgagtgg tgaaccacga tgatattctg gatcccgaat agatgctgcg cgacggtgat
      961 agaccgaagc gcgtcgacgg cacggccgcc ggcaaagcac ggcaaagatc gaagcctcat
     1021 ccgacaattc ccggacctga aggtggagca gcgcacagcg ccatgctcga acggcatgcg
     1081 gggcagcacc gctcgtcgag ttccaccgcc aggcatgaag caattccccg ttgggcatag
     1141 tcacaagcag gggctggagc tgattacgcc cggcacggac ttgcgatgga tggggggtaa
     1201 gaagccatga gagccggctg tctcctcgac tggggcgata gaggttgtcc cgagatgtct
     1261 actatttgtc tgctttcggg gggttgcact cggattcggg ctggcgtttc acgaaatgcg
     1321 attctgggtt tatatgctgc attctcgccc ttatggttgg tttcgatcac actgtccgct
     1381 cattcatccc cccgcagcga accgcttctc gatctcgtcc atcagcgggt gggtgagccc
     1441 ggccttgcgc agttcggcaa agtcctcaag cacgacggtc tcccagggct tatcggccgc
     1501 cacgttcttc tcgccgcggt actgaagata gagtgcccgc gcctcgtcca cgcgtgcgag
     1561 gaacatcagc gcatgagcgc ggttcgtgta gagccatatc tggtcaggcg cgatcgagat
     1621 gacgaggtcg gaggcttcca gcgctctcgt gaaatcacgg gccaagacga gctcgttcgc
     1681 taagtcgccg atgctgctga tgctaccctg caaattctgc tgccactggg tattgctgcg
     1741 gtcggcggcg gcgaggcgct cggcgatggc gaggctgtcg cggtggactt tgagcgcctc
     1801 ctcgagcttg ccctgcgcca ccagcacgtc gccgactctg ttgtaggcga aagacagatc
     1861 actttgccac tgcgtgttac tgcggtcgac atcggccagg cgcttggcga tagcgaggct
     1921 gtcgcggaag gtcttgagtg cctcgtcgag cttaccctgc gccttaagca cgtcgcctac
     1981 cctattggat gcgaacgaca catcgcgctg ccactgcgta ttactgcggt cgacatcggc
     2041 caggcgctcg gcgatagcga ggctgtcgcg gtagaccttg cgcgcctcct caagcttgcc
     2101 ctgcgccacc agcacgtcgc cgaccctgtt gtaggcgacc gccagattgc tctgccactg
     2161 cgcattgctc ggatcggcgg cggccaagcg ctcggcaatg gcgaggctgt cgcggaaagg
     2221 cttgagcgcc tcctcgagct tgccctgcgc cgtcagcgcg tcgccgaccc tgttgtaggc
     2281 gaccgccaga ctgctctgcc actgcgtatt gctcggatcg gcggcggcca agcgttcggc
     2341 aatggtgagg ctgtcgcgga aaggcttgag cgcctcctcg agcttgccct gcgccttcag
     2401 cacgtctccg agcttgacgt atgaccacgt cagaccgctc tgccattggg tgttgctggg
     2461 atcggcggcg gccaagcgct cggcaacggc gagactgtcg ccgtatacct tgagcgcctc
     2521 ctcaagcctg ccctgcacca ccagcacgtc tccgaggttc atgtatgacc acgccagatc
     2581 gcgctgccat tgcgtattgc tccgatcagt ggcggccagg cgctcacgaa tgacgaggct
     2641 atccctgtag gtcttgagcg tctcctcgag cttgccctgc tgtaccagca cgtaaccgat
     2701 cgtattgaaa gcagtcgcca gatcgctctg ccattgggta tcgctcggat cagtggcggc
     2761 caggcgctca cgaatgacga ggctatccct gtaggtcttg agcgcctcct cgagcttgcc
     2821 ctgcgccaca agcgcgtcgc cgaccctgct gtaggagaac gcaagactgc tttgccgttg
     2881 cgtattgctg cgatcagtgg ccgcaaggcg ctcggcgatc gcgacacttt cgcggaaggt
     2941 cttgagcgcc tcctcgagct tgccctgtgc cttcagcacg tcgccgatcc tgacgtagga
     3001 ggccgacacg tcgctctgcc actgcatgtt actgcggtcg acagcggcca gccgctcgga
     3061 gatagcgagg ctgtcgcgga aagccttgag cgcctcctca agcttgccct gcgccaccag
     3121 gacgtcaccg acatcggtgt aggcagacga cagatcgcgc tgccattgcg tattgctccg
     3181 atcagcggct gtgaggcgct cgccgatggc gaggctgtcg cggtaggtct taagggcctc
     3241 cccgagcttc ccctgcgcct taagcacgtg gccgaccgta gcgtagaagg ccgccagatc
     3301 gcgctgccac tgcgtattgc tgcgatcagt ggcggcgagg cgctcggcga tcgcgagact
     3361 ttcccggaag gtcttcagcg cgtcctcgag cttgccctgt gccttcagca cgtcgccgac
     3421 cttgatgtag gagaccgaca aatcgcgctg ccactgcgta ttgctgcgat ccgtggcggt
     3481 gagacgttcc cggatggtga ggctgtcgcg gaaggtcttg agcgcctcgt cgagccttcc
     3541 ctgcgcccta agcgcgtcgc cgaccctgtc gtaggaggcc gtcagatcac gctgccactg
     3601 cgtgttattg cggttagcag cggccaggcg ctcggcgatg gcgaggctgt cccggtaggc
     3661 cttgagcgcc tcttcgagct tgccctgcgc cctaagcacg tcgccgacct cgatggagga
     3721 gatcgacagg tcgtgctgcc actgcatatt gctgccatcg gcggcgatga ggcgctcgaa
     3781 gatggtaacg ctgtcgcggt aggccttgag tgccgcgtcg aggtcgccga gtgtctgata
     3841 cgttttgccg aactcttcaa gcatcgtcga ttggctatat tgcaggtcga gatcatttgg
     3901 cgccgaggcg gcgagctgct cgaaggtagc ctttgctgcc tcgaggatct tgcgcactgt
     3961 gtccgctggc atgcccagga cgttgcgcaa acctcgggcg atgtcgacca ccaggctgtc
     4021 ggcggtgcgc ttggcaagtt tgaagttttg tgtcgcccga tcacgctcgg caaccgcgcg
     4081 cgcttcggcc tgaacggctt cctgctggga ccggtaggcc cagatcccgg ttgcggtggc
     4141 cgcaaccgcc agaatgaaga agaatatggc agctgctgcc accagccgct gtcgcacccg
     4201 cgcccgattg cgggacgtct tcacgtacga ggtgaactcg gccggcagtt ccgcgccgaa
     4261 atcctcgacc agcttctcgg cctcggcgag tgggacaccc ggctggatca accggtcctt
     4321 cggacggcca tgctcctgcc agagccgcag cgcatcctcg acctcagccc gtacccgata
     4381 gaactcgcgg ctcgcttggg cggcctcgct ggccctcggc caactcgcta agaccgcatc
     4441 gtgcgctagc cgcaccgtcg gccgtccctc cgcatccgtg cgcgcgatca ggatgcgcgc
     4501 catcagcagt gcatcgacca gcgtggcctc cgacgcctgg ctcgcgacgt cggcatgtga
     4561 cacctctcgc agcgttaagg tcttgccgtc tcgtgccggc tcggcaagcc gccgcagcaa
     4621 ttgcggtagc gtggcgacag cttgcgatgg caggcttgat accgcgcgct ccgcttccgc
     4681 tgcgatcgca cccgcaagcc cgccgagctg gtcataggcc gcgtgggtca gcctggcctc
     4741 gccagcgatc tcctctcgcc gctcgtagag ctgccgcagc gtgaactgca ggagcggcag
     4801 gctgtcggcg gtcttggcgt cggcgagcag ccgctcgcca agctcgccct tctcagtatc
     4861 cgtctcgaac accagcccgg ccgctgcggc tggcgcgtgc acgatctcag cgagttcggc
     4921 cggtccgggc gggccgagat cgaggcttgc gcctgtctcc ttcagggctt tcaacgccgg
     4981 ctgcttcagc agcagctcgt agaggtcggc gcgcaatgtt gccaccatcc acacccgccc
     5041 agtcgcgatg agctggcgca ggctctcggc aaaggccacg cgctcagcat cactgaccct
     5101 ctgggcgaac agctcctcga actgatcgac tagcagcaca agcacaggct tcggtggctg
     5161 atccgcatga cgctcccgtt gggcgtcttc agccacgcgc gcgagcgcac tggtgatcgg
     5221 ctgcactgaa gcggttccgc cgcgtcggag gttgtcggcg aggctggccg cgctcggata
     5281 atcaccgtgc accagttcgg gtaatgcgtc cgccacaagc agggccatcg ccagggccat
     5341 gagcgggccg gcctggccct cgccgggctt cattcgcgcc acccgccaca aatcgaccga
     5401 cgccaccacc ccgggcgtgg tcaaccgcgg gatcaggccg gcgcgagcca gcgaggactt
     5461 gcctgacccg ctcgccccaa cgatcaggag gaatgggcag ccgcgctctg ctgctcccac
     5521 aaaccgccgc cgggcctcat cgttcactcg gtcgcggccg aagaatactg ccgcatgttc
     5581 ggcctcgaac ggggccagtc ctcgaaacgg cgaacccttc tccttaggcc aggctatccg
     5641 cggaccaagg aaaccacgcg tctcgagcca ctgccgcagc agcagctcga cctggtgctc
     5701 gaatacatcc gtgctgagga aattctgaaa ggcggccttg aactcaccct tctccgacct
     5761 gaaccattcg ctccagaatg cctcgagcgc gtcgagctgg gtctgcgctt gccggcgccg
     5821 ctccacgtcc gccatcggca acgtcgcatc ggcagtcttg cggaacacat agacatctgg
     5881 cacgcccccc gccttcgagg cctcgagggc tgtgagcagc tcgtaggccg tccccgacgg
     5941 atagggcttc ccgtttggca tgcgggggaa gtcggcggga accgatgcgc gtccagaaga
     6001 tcgacactac aatgtcgctg gcgaccgctg ccggaatctg gggctggaaa ctcttggcgg
     6061 ctgtatagaa atgctcctcc cagcgcaccg tctcgaacca aacaaggcct tcatattcgc
     6121 ggttgagctt ggcagtgacc gcctgcacgc ggccgcgctc ggccgccacg tccttcggcg
     6181 aggacacgaa gatcgtgact ttgcgcaccc gcgtgtccgg catggcggag cttcaccata
     6241 aagagcacgg gatcagcttg gatattctat acgatctcgc aacgggaggt tgaaacaacc
     6301 aggttgtaag ttatctaaac gctggtatag tatccttaca gttcggctct aagggcccgc
     6361 aaatgccggc gcaccaaggc aaattcatcg cctatttccg ggtcagcact gacaggcagg
     6421 ggaagtctgg acttggcctt gaggcgcagc ggaaatctgt ccttgactat ctcgacggtg
     6481 gtcgatggaa cctcgtcggg gagttcactg aggtcgagag cggtaagcac aatgatcggc
     6541 cagaattgaa gagcgcgctt gcagcgtgca agaagcagaa ggccaagcta gtcattgcca
     6601 agctcgatcg cctatcccgt aatttggcat tcatcgcgac gttgatggat tccggcgtgg
     6661 agttcatcgc ggtcgataac ccgcacgcga acaagttgac aatccatatc ttggccgcgg
     6721 ttgcgcagca cgagcgcgag ataatcagtg cacgcacttc cgctgccttg aaggccgcca
     6781 aggcgcgggg caaacgtctt ggcaacccga aactccccga agcccgacgg cgtgcggcct
     6841 tggccaagaa ggaaaaggct gaccgatatt cagcaaacgt gttgccagtc attcgtgaaa
     6901 ttcaacgaag tggcatcaaa tcgcttcggg gcatcgcgcg cgcattggca gcgcgcggta
     6961 ttcgaacggc tcgcggaggc gcatggacgc cggtgcaagt aactgcaatc ctgcggcgtg
     7021 ccggctgacc ggatcaggcg cgactcattc caggccgata tgcacggtct tggtttccat
     7081 taggtaggag cgctcctcaa tgggcgatta cgacaagatg gcggaggagc gccgacagct
     7141 atcattacag tcacatagag cacccccttc ggaagggggt acctaaaggg agagcgggcc
     7201 tatggcgaac gtgacattga ttcgcgctct atacgattta agcgtgtgta gcgcccttgc
     7261 gaatttctgc tcgactgccg agtcggcggc aaaagcgtga agtcggttcg tgacatttcg
     7321 agacgcaacg aagcacgaac aggacatctc agtccggtgc gaaatccgcg cgtttggcga
     7381 tcgggcggaa aattagccaa ctctctcaac gatagagcac tccctaacca agggggtgcc
     7441 aattttcttg tttggtaaag gatgaaaaga cagtcactgt cgtgccttcc gaagtttttt
     7501 gttcgaggtg cacgtcatgt ctactaagcg acccacgctc aatcgtctca cagcttatgc
     7561 ctggtatctc ggctctgcgt tctggcggga acgccgcgag cacatcctgc agcgcgccaa
     7621 ctacacttgc gagaaatgcg gtaggcggcc agcaaccgag gtccatcacc taacttacgt
     7681 gcgcgtgttc caggaactac cggctgattt ggtcgctctg tgcaagcaat gccacgcgga
     7741 aattcattgg cagcaaccgg cgaacgacaa tcagctgcaa tgctccttcg atccccccac
     7801 agcgctggag gaggagtcct gatgggttgc tatttcctgt ccaatatctt ggccgaatgg
     7861 tagcccaaaa gagttcaatc gtgatctcct ctttttttac aacagatcag gcggctgagc
     7921 ggcttcgcaa gagccgcaaa tggttgcagg agtggttgtg cgagcacccg atcagctggt
     7981 cgggcatgcc gtattacatg gctattgggc gcacaaagct tttcacagac gaaaatattc
     8041 gttggatcgc gcgggcaatc gacgatcaag aagctgaacg aaaggaacgc aacgaggcgg
     8101 ccaagaacga tgacggcttc atctacttca tagaagctgg cgacttcatc aaaatcggcc
     8161 acacgcgatc accggtcggg cgaggccata agatgcggac cgacaatcct tttgatctga
     8221 aattgctcca catcgaggat ggcacattta aaagggaaaa gctctaccat cgacactttg
     8281 cggcaatccg agtgcgcggc gagtggttcc acaagacacc tgatctcctc gccttcatcg
     8341 aacagcgtaa gcggatcgtc gcgggggagc cataacgggg cggccgggcg agacgaactt
     8401 gtcccgccgc cgcctggcgc gttccagttg accggcgcat cgaccggttc atcatcggtt
     8461 gtcgtggtaa cactggtaag gtgagatact gagagaagct cttctgagcc gtcatccgac
     8521 cgatcgtcaa cttcccgaag gtctgcccca agcggaagca catgaccctg agccttcacc
     8581 gtcaaccgtc acgttcggtg cgtggttacc aacaagttaa aacgcaacca caatcccttg
     8641 aaatggcttt ggcatggcgg tgcagatgcg acctgcgcgc cagtctcagc ctcaacgtaa
     8701 ttaccagagc tcaagcatcg acgtcaggcc gagcccatta cggccctcga acgtggcggt
     8761 ctcgaatttg aggcctaatt cgcgcgcgtg gcgccaggaa tcatcgagct ccgttcgcgt
     8821 cggcgggcga gcgatttccg cgtatttgtc ccggtatttg gcgctcgccg gatcgcagaa
     8881 attgtcggga cggaattgcg ccatcacact caccggagcg gccggtgtgc gctcggcaat
     8941 ccattccagt accggatacg tgcagcactc gacgtggttg ggcataacca agtgtcggat
     9001 cgaaaaatct tcgccccaat cggccagcag cgcgatgttg cgcgtcaccg tctcccagta
     9061 ccacggcgtc ttcgcgagcg tcattgcgca acggccaggt ccgaatttga agtcgggcag
     9121 ccagacgtca gtgagcacgc gtaggatctt catgctttcg agtgtcatga agaaattgct
     9181 gttccatagc atcggcgcat tgaacttgcc ctcataggtg gcggcgcccg gtacctcgtc
     9241 gaaccagagg aagcggtcgg ccttggtctt acgggcccgt ttgagctccg cgggtgtagg
     9301 cgtgaagtcg cggcccaaca atgcgatagc gtcgacgata gcatgcaggt gaatgacgac
     9361 ctcaccgccc acccaattga tattgtggca accttcgcgg cgcagcgtcc acgccatcgc
     9421 cgcgagtgtg cgcgggtccg tctcctcgcc gttgtctttg tcagtgctga tgtccccatt
     9481 ctggcagaag gcgcaccgca tgttgca
//
LOCUS       DATLJT010000013         4372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4135992,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000013 DATLJT010000000
VERSION     DATLJT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4135992"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(803..1618)
                     /locus_tag="VM910_00260"
     CDS             complement(803..1618)
                     /locus_tag="VM910_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI61018.1"
                     /translation="MTRPIPHSGEELPIVGLGTAVSFPSADESQRAALKDVIDALVAG
                     GGKLIDTASVYGNAESVIGDIVRASNARNKIFIATKIEVRSAKAGADEFQRSLQRLRA
                     ENVDLLQLHNVSRASQSLSQFREWKAAGRCRYVGITSTFSADYNAMEAIIRREKPDFV
                     QVNYSMDNRAAEKRILPAAADAGAAVLTAMPFGKTSLFRAVKGKSLPDWAKHFDAATW
                     GQFFLKFLLGNKQVTAVISGTGNADHMIDNLGAARGRLPDAQQRERMAALLAS"
     gene            complement(1835..3193)
                     /locus_tag="VM910_00265"
     CDS             complement(1835..3193)
                     /locus_tag="VM910_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61019.1"
                     /translation="MTESDADAAPASNNPFHRLLRRLINVTPAEAPALGWCWLYIFSV
                     LASYYILRPIRDQMGVAGGVNNLPWLFTGTLIAMLVLNVPFSALVKFLPRKQFISLSY
                     RFFAASILAFGAALHWATPEQVVWIGRFFFIWISVFNLFVVSIFWSMVVDIFNSDQGK
                     RLFGFIAAGATLGAIVGSSVTVSLAQLVQPPFLLVGAAILLEVSTFCVRRLSQLSEKM
                     SDRPHPEREEEPIGGGVMAGFVDAVRSPYLLNTAIFLLLYAVTSTFLYFSQASVVSES
                     FTSRGAQTTFFATIDLSVNVITLVVQIFFTGRLVRWLGVAVVLGSLPALSVLGFGAIS
                     VVPTLAAVASFQVLRRAGNYALSQPARQVLYTVVSREDRYKAKNFIDTAVYRAGDQVG
                     AWSYALVGSIGWGIKEAGVIAIVLSALWLANSLWLGKRQETLAEQQADAERNNAEAAA
                     TA"
     gene            complement(3190..3930)
                     /locus_tag="VM910_00270"
     CDS             complement(3190..3930)
                     /locus_tag="VM910_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HVI61020.1"
                     /translation="MTDDVLDHSRRKFFRFGAAVLFASVTPTHAMAQSVSNKLKIGVI
                     GSGHIGGTIGGLWVKAGHSVLFSSRHPEELKPLTDSLGPLAKAGTVGDALDFGDVILV
                     AVPYKAIPELAKDYGPKFAGKIVIDPANAVARRDGEDLLKETKEKGIGATTQSYLKGS
                     HVVRAFNSMSYTNFVKEAHRAGDPMAIPIAGDDARAVQVASQLVRDAGFEPVAVPLAR
                     AQEFAQGGPLYGQQLTGKALRERFGLVK"
     gene            complement(4155..>4372)
                     /locus_tag="VM910_00275"
     CDS             complement(4155..>4372)
                     /locus_tag="VM910_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61021.1"
                     /translation="THGTGRFNLAQGIIGTASGLGAALSTALFGFVAANFDWAATFLS
                     MASVSLLAISIMWLLMPETRPSTEPQA"
BASE COUNT          924 a         1323 c         1292 g          833 t
ORIGIN      
        1 gcggcgagaa ttgttatttg ggcagtgcgc gaacgaaaca tcatcttgga tccgtccctt
       61 ggtgctgcag ccgactatgc tagacctagg atccgtgcga gcggtcatta ttggcgccac
      121 ttctccgcgg atcgagcaca tcttaaccca acgcctgcgg cttggtgaaa atcattgacg
      181 ttgtgcgcca tcaacaatgc gcgcacaact tctccacgac ggtgagactg ttcggctggt
      241 tttttcgcca tcaagcgcag catgctgctg accgtggagg gtgagcgcga cgacatttgc
      301 gccgtcggtc agcggcccgc tccggggctc acgaccacgc aaatcctatt caaacttcac
      361 caggagcccg cccgggctgg ccaaattgga ccgggcaaac ttcctgctta ccagccaacg
      421 tgggctttag ctcgccggac gtgcgggcgc gatcgatggt ggcggcaatg ttctggctgg
      481 tcgtcggcca ccatcatgtg aggattgaga gcggcaaaca cacggatcag ttccgtccag
      541 tcgtcaggtg cggcggcgct taatacccct ccactcgtcg aacggcggtt attggccagg
      601 gtagttgtta acgcgcgcat gatcaggtta cgttgcttct cgttaattcg ttgcccccgg
      661 tgtcagataa aaggcgcagt ccggcgggcc ccaccagctg acgatgcacg ttccgctgcg
      721 ccgttcggac cggagaaaga cttcattcaa atctgtccgc gagccgatag agtgttccac
      781 catctagtgt gtgtgagccc tttcacgaag cgagaagcgc cgccatcctc tcgcgttgct
      841 gtgcgtccgg taaacggccc ctggccgcac ccaggttatc aatcatgtga tcagcattgc
      901 cggtgcctga gatgacagcg gtgacttgtt tgttgccgag caggaacttg agaaaaaact
      961 ggccccaggt ggctgcgtcg aagtgcttgg cccagtccgg caagctcttg cctttaaccg
     1021 cccggaacaa tgacgttttc ccgaacggca ttgcggttaa caccgcggct cccgcatccg
     1081 ctgccgctgg aaggatgcgc ttctctgcgg cgcgattgtc catcgaataa ttaacctgga
     1141 caaaatccgg cttctcccgc cggatgatcg cttccattgc gttgtagtcc gcactgaacg
     1201 tgctggtaat cccgacgtag cggcaacggc cggcggcttt ccactctcgg aactgcgata
     1261 ggctctggct ggcgcgcgag acgttgtgga gttgcagcaa atcgacattt tcggcccgca
     1321 ggcgctgcaa ggatcgctgg aattcatccg cgcccgcctt cgcggaccga acctcgattt
     1381 tcgttgcaat gaatatctta ttgcgggcgt tgctcgcgcg aacgatgtcg ccgattacgc
     1441 tttctgcgtt gccataaacc gacgccgtat cgattagctt gccgccaccg gccacaagcg
     1501 cgtcgattac gtctttgagc gcggcgcgct gcgactcatc ggccgacgga aagctcaccg
     1561 cggtgccaag cccaacgatg ggcagttcct cgccggaatg agggatcggt cgggtcatca
     1621 cggactcggt gtttttgtgc ttgtgatcgg ccgacaagcc aagacgaaga aacggccaaa
     1681 ccggctgcca acaccgagcg acgcgaccat ccaagcatgt tgtccatatt gcctccacgc
     1741 aacgatagcc actgatgagt aatgtaagtg gcggcgtcat ggtttgtagc ggtgtggaac
     1801 cgctcgtcca gcgagcgacg cgcgccggac acggtcacgc tgtcgctgcc gcctcagcat
     1861 tgttccgctc ggcatccgcc tgctgttcgg ccaaggtttc ctgtcgtttg ccgagccaga
     1921 ggctattggc gagccaaagc gccgaaagca caatagcgat gacgccggct tccttgatcc
     1981 cccagccaat agatccgacc agcgcatagg accaggctcc cacctggtcg ccggcccggt
     2041 acaccgcggt gtcgatgaaa ttcttcgcct tgtagcggtc ttcccgggac acgactgtgt
     2101 acaagacctg gcgcgcgggc tgactgagcg cataattgcc ggcacggcga agcacctgga
     2161 aggacgcgac cgccgccaag gtcggaacca ccgagatcgc tccaaagccc agcacactca
     2221 gtgccggaag tgatcccagc accaccgcaa cgcccagcca gcgcaccagg cgcccggtaa
     2281 agaaaatctg cacgacaagt gtgatcacat ttacgcttag gtcgatggtg gcaaaaaagg
     2341 ttgtctgcgc gccgcggctg gtgaagctct cggagacgac gctggcctgg ctgaagtaca
     2401 ggaacgtgga tgtcaccgcg tagagcaata agaagatcgc cgtgtttagc agatagggcg
     2461 agcgcacggc atcgacgaat cctgccatca caccaccgcc gatcggttcc tcttcacgct
     2521 cgggatgcgg ccgatcgctc atcttttccg agagctgaga cagtctccgc acgcagaagg
     2581 tcgatacctc aagcagaatg gcggcgccga caagcagaaa gggcggctgc accagctgag
     2641 ccagagaaac cgtcacgctc gacccgacga tcgcaccgag ggttgcgcct gccgcgatga
     2701 atccgaacag ccgcttgcct tggtcggagt tgaagatatc gaccaccatc gaccagaaga
     2761 tcgacacgac gaaaaggttg aacaccgaga tccagataaa gaagaatcga ccaatccata
     2821 ccacttgctc cggcgtggcc cagtgcagcg ccgcgccgaa cgccaggatg gaggcagcga
     2881 agaaacggta ggacagtgag atgaattgct tgcgcggcaa aaacttcaca agagccgaaa
     2941 acggcacgtt caagaccagc atggcgatca acgtgcccgt gaacagccat ggcagattat
     3001 tcaccccacc ggccacaccc atctggtcgc ggatgggacg caggatgtag tacgaggcaa
     3061 ggaccgagaa aatgtagagc caacaccagc cgagcgcagg agcctcggct ggtgtcacat
     3121 ttatcaggcg acgaaggagg cgatgaaacg ggttgttcga cgccggcgcg gcgtccgcat
     3181 cggattcggt catttgacga gaccgaagcg ctcgcgcaat gccttgccgg tgagctgctg
     3241 gccgtagagg ggcccgccct gcgcgaactc ctgcgcccgg gcaaggggca ccgccactgg
     3301 ctcgaaccct gcatcgcgca ccagctgcga ggcgacctgg accgcgcgcg cgtcgtcgcc
     3361 cgcgattggt atagccatcg gatcgcccgc acggtgtgct tccttcacga agttggtata
     3421 gctcatcgag ttgaatgcac gcaccacgtg agagcccttg aggtacgatt gcgtcgtcgc
     3481 accgatgcct ttttctttcg tctccttcag caggtcctcg ccgtcgcggc gggcaaccgc
     3541 gttggcaggg tcgatcacga tcttgcctgc gaacttggga ccgtaatcct tggcaagctc
     3601 cgggatcgcc ttgtagggca cggccacgag gatgacatcg ccaaagtcca gcgcgtcacc
     3661 aaccgttcca gccttggcaa ggggcccgag gctatcggtc agaggcttca gctcttccgg
     3721 atggcgcgat gagaacagga ccgagtgacc ggccttgacc cataggccgc cgattgtgcc
     3781 gccgatgtgg ccacttccaa tgacgccaat ctttagctta ttggaaacgg attgagccat
     3841 ggcgtgcgtg ggcgtcacgc tcgcgaacag gactgcggca ccgaaacgga agaatttgcg
     3901 acggctatgg tccaacacat cgtcggtcat gactcatctc caaatgcccg gtactgggag
     3961 gggtatagat gcggaccata gtagccagag aaggcgtcct caggagattt acggtcatca
     4021 attcggtgtg agtgtgcctg cgcagcgact tcgcgagtgg gtcttcgctg ctcgcccgcg
     4081 ccggaacctc aagcgcacgc gcgaacggtg caaagagccc cagtccgcgg tggaccgttt
     4141 gcctgcgacc gtcattaggc ttgtggttcg gtcgacggtc tcgtctctgg catcaagagc
     4201 cacataatcg aaatagcaag caagcttacc gatgccatac tcagaaatgt tgccgcccaa
     4261 tcaaagttcg cggcgacgaa cccaaagagt gcagtgctga gcgcagcacc aagtcctgac
     4321 gcggtcccaa tgatgccttg agccagattg aaccgacccg tgccatgcgt aa
//
LOCUS       DATLJT010000014         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1149379,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000014 DATLJT010000000
VERSION     DATLJT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149379"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VM910_00280"
     CDS             <1..1122
                     /locus_tag="VM910_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI61022.1"
                     /translation="RGANLWLGRNEECGIRCVYHGWKFNTEGACVDMPTSYPDLNAKD
                     LIRIKSYPVREWGDMIWAYMGPSGVVPELPALEMALVPASHRYVSKKWQDCNWVQALE
                     GSIDTAHFTFAHLAFEKEEDEILDIKKHFINPLARMSTDHMRWIAEDPRPVIKILPHE
                     AGLTVAGGRLSGSDNIYWRIAQFLMPVHAYAPSAMPGDNIFGQSFVPVTDTNCWIYTY
                     AWNPERPLTQAEHDAYDAGNGVIAEVDDNYVPLRNKSNNYLIDRKLQKTKSYTGIKGV
                     SEQDAAVQDSQGPIADRTREHLGPTDLGIMHFRKLVMDAARALERGEVPPHIAHQDRY
                     TVRSGACVTSKAKDLPAVMIERFGDVAGFVGPPRIAAAE"
     gene            1127..2101
                     /locus_tag="VM910_00285"
     CDS             1127..2101
                     /locus_tag="VM910_00285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVI61023.1"
                     /translation="MLSSGGRLAPSGRRTILAALAALLLLPMEASDAGWLQDVFKGSP
                     KHDKSAKHVKPKSSASAKPAPSSKPHNVKLAALGPASIALPALKPPAPMCEPSKFRIV
                     LDVGHTAQSEGAMSARNVAEFTFNLRLAQRIEEKLKAEGFAETRLLVTQGKARPSLVK
                     RVDAANNLRANLFLSIHHDSVPDSLLEKWEFDGKKSHFSDRFSGYSVFVSHENPDFKT
                     SLVFAELVGKEMKAQGLQYAQQYTLPIMGHYRHPLLNKETGVYRYDELIVLRTTRMPA
                     VLLEAGSIINRDEELKMSSPERQEAISNSVTAAVKEFCDPRWGILGPL"
     gene            complement(2109..2420)
                     /locus_tag="VM910_00290"
     CDS             complement(2109..2420)
                     /locus_tag="VM910_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI61024.1"
                     /translation="MSVYIVAQLKFTRRELYDRYQARFFEVFRKFKGRLLIADEQPMV
                     LEGSFERDKVVVLEFPDREAAMEFHNSPEYAAIAVDRKAGADAIVLMLRGWQPPVKTG
                     S"
     gene            complement(2511..>2724)
                     /locus_tag="VM910_00295"
     CDS             complement(2511..>2724)
                     /locus_tag="VM910_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HVI61025.1"
                     /translation="QLPQSLDPDTCDVPVAGAALTRELIAAERKLIHALLRDGRITDE
                     ARRRIERDLDLEEASLANREYRATPL"
BASE COUNT          539 a          846 c          826 g          513 t
ORIGIN      
        1 cgcggcgcca atttgtggct cggccgcaac gaggaatgcg gtatccgctg cgtctatcat
       61 ggctggaaat tcaacacgga gggcgcttgc gtcgacatgc cgacctccta tcccgatctc
      121 aacgccaagg acctgatccg catcaaatcc tatccggtgc gcgaatgggg cgacatgatc
      181 tgggcctata tgggaccttc cggcgtcgtg ccggaattgc ccgcgctcga gatggcgctg
      241 gtgccggctt cgcatcgcta cgtctcgaag aaatggcagg actgcaactg ggtgcaggcg
      301 ctggaaggct cgatcgacac cgcgcatttc accttcgcgc atctcgcctt cgagaaggaa
      361 gaggacgaga tcctcgacat caagaagcat ttcatcaatc cgctggcacg gatgtcgacc
      421 gaccacatgc gctggatcgc cgaggatccg cggcccgtca tcaagatcct tccgcatgag
      481 gccgggctca cggtcgccgg cggccggctc agcggcagcg acaacatcta ctggcgcatc
      541 gcgcagtttc tgatgccggt gcatgcctac gcgccgagcg cgatgccggg cgataacatc
      601 ttcggccaga gcttcgtgcc ggtcaccgac accaattgct ggatctatac ctatgcctgg
      661 aatccggagc gtccgctgac gcaagccgag cacgacgcct acgatgccgg caacggcgtg
      721 atcgccgagg tcgacgacaa ttatgtcccg ctgcgcaaca agtcgaacaa ctatctgatc
      781 gaccgcaagc tgcagaagac caaaagctat accggcatca agggcgtctc cgagcaggat
      841 gccgccgtcc aggacagcca gggcccgatc gccgatcgca cccgcgagca tcttgggccg
      901 accgatctcg gaatcatgca tttccgaaaa ctggtgatgg atgcggcacg cgcgcttgag
      961 cgcggcgagg tgccgccgca tattgcgcat caggatcgct ataccgtacg gtcaggtgcc
     1021 tgcgtgacga gcaaggcaaa ggatctgccg gccgtgatga tcgagcgctt tggggacgtg
     1081 gcgggtttcg tcggcccgcc gcgtattgca gcagcggagt aggggattgc tgtcgtcagg
     1141 gggaagactc gcgccgtcgg gccgccgtac cattcttgcg gcattggccg cattgctgct
     1201 tctgccgatg gaagccagcg atgccggctg gttgcaggac gttttcaagg gttcgccaaa
     1261 gcacgacaaa tcggcaaagc acgtcaagcc gaagtcctcc gcctcggcga agcccgcccc
     1321 ctcgtcaaag ccccacaatg taaagcttgc cgcgctcggg ccggcctcca tagccctccc
     1381 cgctttgaag ccccccgcgc ccatgtgcga accgtcgaaa ttccgcatcg tgctggatgt
     1441 gggccatacc gcccagtcgg agggtgcgat gagcgcccgc aacgttgccg agttcacctt
     1501 caacctgcgc ctcgcgcagc gaatcgagga gaaactgaag gccgaaggct ttgccgagac
     1561 caggcttctg gtgacgcagg gcaaggccag gcccagcctg gtcaagcgtg tcgacgcagc
     1621 caacaatctg cgggctaatc ttttcctctc gatccatcat gattccgtgc ccgactctct
     1681 tctcgagaaa tgggaattcg acggcaagaa aagtcatttc agcgaccgct tcagcggtta
     1741 ctccgtcttc gtctcgcacg agaatccgga cttcaagacg agcctcgtgt tcgcagaact
     1801 ggtcggcaag gaaatgaagg cccagggcct gcaatatgcg cagcaatata cgttgccgat
     1861 catgggccac tatcgacatc cgctgctgaa caaggaaacc ggcgtctata ggtacgatga
     1921 gttgatcgtg ctgcggacga cccggatgcc ggccgttttg ctggaggcag gctcgatcat
     1981 caaccgcgat gaagagctca agatgagttc gccggagcgc caggaagcca tcagcaacag
     2041 cgtcaccgcg gctgtgaagg agttctgtga cccgcgatgg ggcatcctcg gtccgctctg
     2101 attgcccttc aagagccggt tttgacgggc ggttgccagc ctcgaagcat caatacgatg
     2161 gcgtcggcgc cggccttgcg gtcgacggcg atggccgcgt attcgggcga gttgtggaat
     2221 tccatcgcgg cttctcgatc cggaaactcc agcacgacga ccttgtcgcg ctcgaacgat
     2281 ccttccagca ccatcggctg ctcgtcggca atgagtagcc tgcccttgaa cttgcgaaac
     2341 acctcgaaga agcgcgcctg gtatcggtca taaagttcgc gacgggtgaa tttgagctgg
     2401 gcgacgatgt acacgctcat acctttgacc ttactggtta cgtgcgctgc tttgcgagcg
     2461 cggtctatag gaattctgcc tcactcccct tgcacgcgtg aaaagggaaa tcacagcggc
     2521 gtcgcccgat attcgcgatt ggcgaggctt gcttcctcga ggtcgagatc gcgctcgatc
     2581 cggcgccgcg cctcgtcggt gatgcgtccg tcgcgcaaca gcgcgtggat gagcttgcgc
     2641 tccgccgcga tcagctcccg cgtcaatgcc gcccccgcca cgggcacgtc gcaggtgtcg
     2701 ggatcgagcg actgcggcag ttga
//
LOCUS       DATLJT010000015         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2298065,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000015 DATLJT010000000
VERSION     DATLJT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298065"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VM910_00300"
     CDS             complement(<1..560)
                     /locus_tag="VM910_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI61026.1"
                     /translation="MAKFGIGQAVRRVEDQRFLLGQGRYVDDISLPGQCYGVTVLSPH
                     AHARIKRVDVAKAKAAPGVLCVLTGADAVAEKLGSFTAHLMPEDFGAPKGYRTFQPVL
                     NADKVRFVGDRVAFVVAETLSEARDAAELVEVDYEPLPAVVNLEDAAKDGAAKVWDDC
                     PQGNVGFRLMFGNKDTTDAAFAKAKHV"
     gene            complement(709..1662)
                     /locus_tag="VM910_00305"
     CDS             complement(709..1662)
                     /locus_tag="VM910_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61027.1"
                     /translation="MSKSSTTVYWTGFSIVLALLIVAPLVLPEFWRRFVTEILIWGLL
                     AMSSDILIGYTGMVSFGHSAFFGLGMYGAAAALLMVKPPNLWLAIVYGLIGSAGVALF
                     VAYFSTRLRDIYFAITTLIFSQIFYVIIFTWTEVTGGENGLSFRRPPLAIPGLFSVPF
                     SSETLHWFVLAVVTASYLILRRITQSPFGMVLQSIRENEARTRAIGYAVERYKMVAVM
                     LSGLFAGLAGVLYAVQNRFAAPDFVYFLVSGETVIFNVIGGIGTLVGPIVGAGFFLLL
                     REAFSRFFTEYYLIPVGVIFIAMVIFMPQGLLGFMRRWLNQ"
     gene            complement(1659..>2229)
                     /locus_tag="VM910_00310"
     CDS             complement(1659..>2229)
                     /locus_tag="VM910_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61028.1"
                     /translation="TFGISLVLQNYALWQFGPVARKIQEPFTGYFTLFYLEYPWYRLL
                     IAALSAAIIGAFWLFLKYGKYGVWIRATTQDRVMAEAMGIPVPWVHTGAFAIGAGMAA
                     ASGVLFGPLVGVNHTMGLDWILKAFIVVVVGGMGSLGGSIAASIFISLLEAYASIWAS
                     PAQAVIVSFVVLILTLLFRPTGLFVPTPK"
BASE COUNT          473 a          703 c          726 g          327 t
ORIGIN      
        1 acgtgcttgg ccttggcgaa ggcggcgtcg gttgtatcct tgttgccgaa catgagccgg
       61 aacccgacat tgccctgcgg gcaatcgtcc cagactttag cggcgccgtc cttggcggcg
      121 tcttcgaggt tcaccaccgc cggcagcggc tcatagtcga cctcgaccag ttcggcggca
      181 tcgcgcgcct cactcagcgt ctcagccacg acgaacgcga cgcggtcgcc gacgaagcgc
      241 accttgtcgg cattgagcac cggctggaac gtgcggtagc ccttgggcgc gccgaagtcc
      301 tcgggcatca gatgggcggt gaagctgccc agcttctccg ccacggcatc ggcgccggtg
      361 agcacgcaga gcacgcccgg cgcggcctta gccttggcga cgtcgacgcg cttgatgcgg
      421 gcgtgcgcgt gcggcgagag caccgtcacc ccgtaacatt gcccgggcag gctgatgtcg
      481 tcgacgtagc gcccttgccc gagcagaaaa cgctggtctt ccactcgccg cacagcttgt
      541 ccgatgccga acttggccat gtccgcttag ccttctggtc gcgatgtgat gggtcccggc
      601 cgaatgttag tggagacgcg aggctcacgc cagcgcagcc ggcggtccgc cgtgcgcggg
      661 aaatccggag ttctcgtccc tggcgcgacc ggctcgccat cggctccgct attggttgag
      721 ccagcgccgc atgaagccga gcagcccctg cggcatgaag atcaccatgg cgatgaagat
      781 cacgcccacc ggaataaggt agtactcggt gaagaagcgc gagaaggcct cgcgcagcag
      841 caggaagaag ccggcgccga cgatcggtcc caccagcgtg ccgatgccgc cgataacgtt
      901 gaaaatcacc gtctcgccgg acaccagaaa ataaacgaaa tcgggcgccg cgaagcggtt
      961 ttggacggcg tagagcacgc cggcgaggcc cgcgaacaag ccggacaaca tcaccgccac
     1021 catcttgtag cgctcgacgg cgtagccgat cgcgcgcgtg cgtgcctcgt tctcgcggat
     1081 cgactgcagc accatgccga acggcgactg ggtgatgcgc cgcaggatca gataggaggc
     1141 cgtgaccacg gcgagcacga accagtgcag cgtctcggac gaaaacggca ccgagaacag
     1201 ccccggaatg gcgagcggcg gacggcggaa actcagtccg ttctcgcccc cggtcacctc
     1261 ggtccaggtg aagatgatga cgtagaagat ctgcgagaag atcagggtcg tgatcgcgaa
     1321 atagatgtcg cgcagccgcg tgctgaaata tgccacgaac aaggcgacgc cagccgagcc
     1381 gatgaggccg tagacgattg ccagccagag attaggcggc ttgaccatca acagcgccgc
     1441 cgcggcgccg tacatgccga gaccgaagaa agccgagtgg ccgaacgaca ccatgcccgt
     1501 gtagccgatg aggatgtcgg aggacatggc cagcaggccc cagatcagga tctccgtcac
     1561 gaagcgacgc cagaactccg gcagcaccag cggcgccacg atcagcagcg ccaggacgat
     1621 ggagaatccg gtccagtaga cggtcgtgga gctcttgctc atttcggcgt cggaacgaag
     1681 agacctgtcg ggcggaacag cagggtcaga atgagaacca cgaaggagac gattaccgcc
     1741 tgcgccgggc tcgcccagat cgaggcgtag gcttccagca ggctaatgaa gatcgaggcc
     1801 gcgatggagc cgcctaggct tcccatgccg ccgacgacca ccacgatgaa ggccttcagt
     1861 atccagtcca gacccatagt atgattgacg ccgacgagag gaccaaacag cacgccgctc
     1921 gcggcggcca tgccggcgcc gatggcgaac gcgccggtgt ggacccacgg cacggggatg
     1981 cccatggcct cggccatgac gcggtcctgc gtggtggcgc ggatccagac gccgtacttg
     2041 ccgtatttga ggaacagcca aaaggcacca atgatggcgg ccgagagggc cgcgatcagc
     2101 agccggtacc aggggtattc aagatagaaa agggtgaaat aaccggtgaa gggctcctgg
     2161 atcttgcgcg ccaccggacc gaattgccac agcgcatagt tctgcagtac cagcgaaatg
     2221 ccaaacgta
//
LOCUS       DATLJT010000016         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3217101,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000016 DATLJT010000000
VERSION     DATLJT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217101"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VM910_00315"
     CDS             complement(<1..146)
                     /locus_tag="VM910_00315"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HVI61029.1"
                     /translation="MARITLRQLLDHAAENDYGVPAFNINNMEQALAIMDAASSLDAP
                     VIIQA"
     gene            complement(193..1389)
                     /locus_tag="VM910_00320"
     CDS             complement(193..1389)
                     /locus_tag="VM910_00320"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVI61030.1"
                     /translation="MTKQFRTLDDVDVKGKRVLLRVDLNVPTENGHVTDATRLERVAP
                     TITEISDRGGRVILLAHFGRPKGRDPKESLKPVAAALSDVVKRPVAFADDCIGEAAQK
                     AVAAMKDGDILCLENTRYHKEEEKNDPAFTAELAKLGDIWVNDAFSAAHRAHASTEGL
                     GHKLPAYAGRSMQAELEALSRALEAPTKPVIAIVGGAKVSTKIDLLENLVAKVDALVI
                     GGGMANTFLHAQGVAIGKSLAEKDLAPTALRIIDKANEANCAIILPVDATVAYHFAAN
                     SPSHAYGLDAIPPDGMILDVGPQSTARIHAAIDDAATLVWNGPLGAFEMPPFDRGTVL
                     AAKYAAGRTRAKKLISVAGGGDTVAALNQAGVASDFTYVSTAGGAFLEWMEGKPLPGV
                     EVLKIR"
     gene            complement(1501..>2037)
                     /locus_tag="VM910_00325"
     CDS             complement(1501..>2037)
                     /locus_tag="VM910_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HVI61031.1"
                     /translation="LAPVAKVLNDTVGIETGFMTTIHAYTGDQPTLDTLHKDLYRGRA
                     AAMSMIPTSTGAAKAIGLVLPELKGKLDGVAIRVPTPNVSVVDLKIVAKRATDAKEIN
                     EAMKRASEQQLKGILNYTTHPNVSIDFNHDPHSSTVHMDQTKVMNGNLVRVMSWYDNE
                     WGFSNRMADTAVAMGKVL"
BASE COUNT          318 a          639 c          664 g          416 t
ORIGIN      
        1 gcctggatga tgacgggtgc atcgagcgac gaggcggcgt ccatgatcgc cagcgcctgc
       61 tccatattgt tgatgttgaa ggccggcacg ccgtagtcgt tttccgccgc gtggtcgagc
      121 aattgacgca acgtgatgcg agccatctgt cctgttctcc gcgttggggc gttccgccca
      181 atatggccta acctatctga tcttcaaaac ctcgacgccc ggcaagggtt tcccctccat
      241 ccattcaaga aacgcgccgc cggcggtcga aacataggtg aaatcgctgg ccacaccggc
      301 ctggttgagc gctgcgaccg tgtcgccgcc gccggcaacc gagatcagct ttttggccct
      361 tgtccgcccg gcggcatatt tcgctgcaag caccgtgccg cggtcgaacg gcggcatttc
      421 gaacgctccg agcggcccgt tccagaccag cgtcgcggca tcgtcgattg cggcatggat
      481 gcgcgcggtg gattgcgggc cgacatcgag gatcatgccg tcgggcggga tcgcgtcgag
      541 gccataggcg tgcgacggcg agttggccgc gaaatgatag gcaaccgtcg cgtccaccgg
      601 caggatgatc gcgcaattcg cttcattggc cttgtcgatg atgcggagcg cggtcggcgc
      661 gagatccttc tcggcgagcg acttgccgat cgcaacgccc tgcgcgtgca ggaaggtgtt
      721 ggccatgccg ccgccgatca cgagcgcgtc gaccttggcg acgagatttt ccaaaaggtc
      781 gatcttggtg gacaccttgg cgccgccgac gatcgcgatc acgggcttgg tcggcgcctc
      841 cagcgccctg gagagcgcct cgagttcggc ctgcatcgag cgccccgcat aggccggcag
      901 cttgtggccg agcccttcgg tggaagcgtg cgcgcggtgc gcggccgaga acgcgtcatt
      961 cacccagatg tcgccgagtt tcgcgagctc ggccgtgaat gcggggtcgt tcttttcttc
     1021 ctctttatgg tagcgggtgt tctccaggca caggatgtcg ccgtccttca tcgcggcgac
     1081 tgccttctgc gcggcttccc cgatgcaatc gtcggcaaag gcgaccgggc gtttgaccac
     1141 gtccgacagc gccgccgcga ccggcttcag cgactccttg ggatcgcgcc ccttgggccg
     1201 gccgaaatgc gcgagcagga tgaccctgcc gccgcgatcg gaaatctcgg tgatggtcgg
     1261 cgcgacgcgc tccagccgcg tggcgtcggt gacgtggcca ttttcggtgg gcacgttgag
     1321 atcgacgcgc agcagcacgc gcttgccttt gacgtcgacg tcatcgaggg tacgaaattg
     1381 ttttgtcatt ggaggcttcc gttcgtcatg gcggggtttg tcccggccat tcacgtcttc
     1441 ccttcgtgag gcgctaaaga cgtggatgcc cggcacgagg ccgggcatca cggaaatcgt
     1501 ttacagcacc ttccccatcg cgaccgcggt gtcggccatg cggttcgaga agccccactc
     1561 gttgtcgtac caggacatca cccgcaccag gttgccgttc atcaccttgg tctggtccat
     1621 gtgaaccgtc gacgagtgcg gatcgtggtt gaagtcgatc gagacgttcg ggtgggtcgt
     1681 gtagttcaga atgcccttga gctgctgctc gctggcgcgc ttcatcgctt cattgatctc
     1741 cttcgcgtcg gttgcacgct tggcgacaat cttcaaatcg acgaccgaga cgttgggcgt
     1801 cggcacgcgg atcgcgacgc cgtcgagctt gcccttcaat tccggcagca cgagcccgat
     1861 cgccttggcc gcaccggtcg aggtcgggat catcgacatc gcggcggccc ggccgcggta
     1921 aaggtccttg tgcaacgtgt cgagcgtcgg ctggtcgccg gtataggcgt ggatcgtggt
     1981 catgaagccg gtctcgatgc cgaccgtgtc gttcaggacc tttgcgaccg gcgccag
//
LOCUS       DATLJT010000017         1851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_460137,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000017 DATLJT010000000
VERSION     DATLJT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460137"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VM910_00330"
     CDS             complement(<1..291)
                     /locus_tag="VM910_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61032.1"
                     /translation="MRSQTLGRKSRSTGTKTRTRVGQARKPRADLERQLETCRRELAE
                     ALEQQAATSEVLKVISRSAFDLQLVLDTLVESAVRLCDADHAWLFQREGEFFR"
     gene            complement(556..1545)
                     /locus_tag="VM910_00335"
     CDS             complement(556..1545)
                     /locus_tag="VM910_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61033.1"
                     /translation="MKLPRREFLHLAAGAAALPAVSRTARAQTYPSRPITMVVPYPPG
                     GLTDVIGRILAEGMRTSLGQSVIIENVGGANGSIGTGRVARAAPDGYTLVVGIWNTHV
                     ANGATYALQYDVVKDFEPVLLLADAPLLLVASKAVPANDLKEFIAWLRLNPDKSSMGT
                     VGAGSPGHLLGLLLQKETGTRFGLVAYRGAGPAIQDVVAGQIDTTFVNTATSLPQVRA
                     GSIKAFAVTAKNRLAVAPDIQSVDEAGLPGLHFSLWAGLFAPRGTPKNIISKLNTAAV
                     STISDPTIHERLAEQGFEIPSHEQQTPEALGAYQKAEIEKWWPIIKAAGIKAE"
BASE COUNT          361 a          562 c          565 g          363 t
ORIGIN      
        1 gcggaagaat tcgccctcgc gttggaacag ccaggcgtgg tcagcgtcgc acagacgaac
       61 agctgattcc acgagcgtat cgagcaccag ctggagatcg aaagcagaac ggctgatcac
      121 cttgagcacc tcggaggtcg ctgcctgctg ctccagcgcc tcggcgagtt cgcgcctgca
      181 tgtttcgagc tgccgttcca gatcggcgcg tggcttgcgc gcctgaccaa cgcgggttct
      241 cgtcttcgtt ccggtcgaac gacttttgcg accgagcgtt tggcttctca cgcctttgcg
      301 agacgaggcc acctgaaatc gccccatgcc gaggatcagg ccttccgggg ctaagaaaat
      361 agcacgcagg cccgagcgtg tgtgagccag ttcacattaa tcgccccata caaggccacg
      421 ctgccgccgc gccggcgagc agcatgatga actcgcggcg cttggaccgt ggcaattccc
      481 gctgcaaagt gcagaaaagt tctacggtga aatttcatag catcccctct ctgagatgcc
      541 ggctaggaag gacgcttact ccgccttgat gccggccgct ttgatgatcg gccaccactt
      601 ttcgatctca gccttctgat aagcgccaag cgcctccggc gtttgctgct catgcgacgg
      661 aatttcaaag ccctgctcgg cgagcctctc atgtatggtt ggatcgctta tcgtgctcac
      721 ggccgcagta ttgagcttgc tgatgatgtt cttcggcgtg cccctgggtg caaataggcc
      781 ggcccatagc gaaaagtgaa gccctggcaa tcccgcctcg tccacggact ggatatctgg
      841 cgctacggcc aagcggtttt tggccgtgac ggcgaaggct tttatgctgc cggcgcgcac
      901 ctgcggcagc gaagtcgccg tgttgacaaa tgtcgtgtca atctgccccg ccacaacgtc
      961 ttgaatcgcc gggccagcgc cgcgataagc cactaatcca aatcgcgtgc ctgtttcctt
     1021 ttgcagtaag aggccgagga gatggcctgg gctgccggca ccgacagttc ccatggatga
     1081 tttgtcgggg tttaacctaa gccaagcgat gaactccttc aaatcattcg ccggtacagc
     1141 tttgcttgcc acgagtagca acggagcatc cgcgagtagc aagacaggct cgaaatcctt
     1201 cacgacgtcg tactggagcg cataggtagc gccgttcgcg acgtgcgtgt tccaaatgcc
     1261 gaccaccaga gtgtatccgt cgggcgccgc gcgggcgacc cggccggtac cgatgctgcc
     1321 gttcgcgcca ccgacgttct caatgatgac ggattgacct agcgacgtcc gcatgccctc
     1381 ggccaggata cgcccgatca catccgtcag gccgcccggc gggtatggca cgaccatcgt
     1441 gatcgggcgc gacggataag tttgcgccct cgcggtgcgc gacacggcag gcagcgcagc
     1501 ggcgccggcg gccaagtgca gaaattctcg gcggggaagt ttcattggcg tcctcctccc
     1561 tgatcgcctt taatgcacgc gcgtaaaatg ggcgcgggcg cgcgatgcga gcgtggatag
     1621 accgcacaaa cgatggtctg agtgggatgc ccgccgatgg catcacatgc cgctaaaaag
     1681 gggacttcgg caggtcccgc taggcggctc gaaggttagg ctacatcgac cacgcgactg
     1741 ggcggaaggg ggcatgaccg cttgtggcac ttctcggacg tcgagagatg tccgactttg
     1801 gtccgcaaag acttgggtcc gcaaagtagg ccaaagcgga cgttaagcgg g
//
LOCUS       DATLJT010000018         9329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2757630,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000018 DATLJT010000000
VERSION     DATLJT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757630"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VM910_00340"
     CDS             <1..478
                     /locus_tag="VM910_00340"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVI61034.1"
                     /translation="SSLADLAARIDDPGLDVSPQDVLVLQNAGPHASECMPEAGYLPI
                     PKKLAQSGVKDMIRVSDARMSGTAFGTIVLHVTPDSASGGPLGLVRSGDRIRLSVKER
                     RIDLLVEQAELKARATAIKPTQMKPERGYAKLYAQEILGADDGCDFAFLRPAPSGR"
     gene            620..1378
                     /locus_tag="VM910_00345"
     CDS             620..1378
                     /locus_tag="VM910_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI61035.1"
                     /translation="MAVESIFYSVSGMSCRGALIWDEGVKAPRPLLLMAPNWRGVIKP
                     AIETGQMLAGEGYVVFVADMFGEGNGPVGTENPMEFLRPFMSDVAGMRRRIAAALDTL
                     TREADRRAIGDISRRAAMGFCFGGLNVLDLARTGADVQAVVSMHGTLATPQPAKRGDI
                     KAAVLAVHGATDPVAPKAERDAFEAEMTEAGACWGLLTFGDVYHAFTDPQANTAGVAV
                     YDEPATRHGYALAHAFIADAFVGRRHGCSLPRPA"
     gene            1414..2124
                     /locus_tag="VM910_00350"
     CDS             1414..2124
                     /locus_tag="VM910_00350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVI61036.1"
                     /translation="MHRLSIPQSVVDRVIERRGREHVYEDLDPAKTALVVVDMQNAFM
                     LPGVAHALCPMAEKIVPNINRLAQAVRETGGTVVWIKTTFKDDALQSWSTYFEMVTPQ
                     QSAKRIEALTADSKGHQLWADLDVRAEDVIVEKNRFSAFIQGSSNLAEVLRGRGIDTI
                     LITGTVTNVCCESTARDAMMLNFKTVMVTDGNAAVTDEDHNGSLRAFYLMFGDIASTD
                     MLITWLARNAKRNLAAAE"
     gene            2155..3351
                     /locus_tag="VM910_00355"
     CDS             2155..3351
                     /locus_tag="VM910_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61037.1"
                     /translation="MEPARHISAVTPEPTEIVARARAMIPALARRSLEGRRRRRIPDE
                     TIADMQRAGFFRVLQPKRWSGYEMDLHTFYEIQLALAEGDMSTAWIYGVSGVHPWFMA
                     LLDDRAAQEVWRSDTSALICSSLMPAGRATPAEGGYRLSGRWRYASGCEHCDWALLGA
                     MVATDSGRPPEGRIFLLPRKDYGTIDTWEVSGLQATGSWDVTVENVFVPAYRSQSMLD
                     NFLLKGPGQALNSSSLYRLPFGQIFVRGISTAALGALQGMLNAFLDYGKTRVTRAGGR
                     SAENPFVQLLCAETVAAIDEMTNTLHRNFRNLHAYAGRGETPPLEERLRYKFQSTEVT
                     ERCTLLAARIFKATGAAGLAEGLPFGGILADLMAGRQHISNQYEYVGSSWGGVMFGLE
                     NKDLML"
     gene            complement(3429..3653)
                     /locus_tag="VM910_00360"
     CDS             complement(3429..3653)
                     /locus_tag="VM910_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61038.1"
                     /translation="MADDNRSLSSAILSLLVAADQRCPAIIGQAHLLYHARSSVQLPT
                     AAKTLAQLKRDTDDLVRAIAEVEAAMKQAT"
     gene            complement(3646..3963)
                     /locus_tag="VM910_00365"
     CDS             complement(3646..3963)
                     /locus_tag="VM910_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61039.1"
                     /translation="MAPKHQGQARTSEARRPADRASRFIARVDAHLPTLANDGDRRAF
                     LDRQLDGWEHRYACFIATEGASEFATSRADPPQAADFLLTIIGLAARRSALGRDAKAD
                     DHG"
     gene            complement(4058..4660)
                     /locus_tag="VM910_00370"
     CDS             complement(4058..4660)
                     /locus_tag="VM910_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVI61040.1"
                     /translation="MAREKRFKSARLAGKAMGIPVSTYGAHERAQSPGGRDYGPEDAK
                     KYARRFGVTPEWLLTGREPFPTGADESPAAPKVPVVGYVGAGSAAHLYDVAQPDLDEV
                     GGPADAAEDTVAVEIRGDSLGPFFNRWYVFYDDVHRPMTADLIGQLCVVGLADGRVLV
                     KQVQRGRAEGLYNLHSATEKPIADVKIEWAARVNSISRRS"
     gene            4785..5432
                     /locus_tag="VM910_00375"
     CDS             4785..5432
                     /locus_tag="VM910_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HVI61041.1"
                     /translation="MLPLSRGVLPTSQRRASPWTTERTRLLRRRWRQGARVREIAAEL
                     GHGITCNAVISKIHRLGISALSPYGGAPGRRYTANTRPADRPVHAQRAAWWFRKGPLP
                     AWVINARPYVETVGADARIPRHQRRSLLELNDETCSWPVGDPRSSRFFFFCGAQPLCN
                     KPYCAAHCARAYRRRGESTRGVSVLRPRDATSEADRSIMPSSKTATQCRPTGEKK"
     gene            5429..5986
                     /locus_tag="VM910_00380"
     CDS             5429..5986
                     /locus_tag="VM910_00380"
                     /inference="COORDINATES: protein motif:HMM:NF040428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HVI61042.1"
                     /translation="MSERSRQSDMRVRKSIKSRKEPLVERRALPTPERLRRAGPDFER
                     GDTGQITMRDSPLERAFARNALTPEQYSAGQKYRHHWYHAGLADRLESIDLNRVFAID
                     LGAYSGMAKTESQLFHRQRYREAAQAVGKIGSHVLEWAVCREIALEQVGQTLGWNSRP
                     QAYAAAVERMKTALDELCKLWGIGS"
     gene            complement(6102..6575)
                     /locus_tag="VM910_00385"
     CDS             complement(6102..6575)
                     /locus_tag="VM910_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61043.1"
                     /translation="MEKLKQETGADIVRIPFRSGNELVNAVLSGSTPVAILALSNMVP
                     QLQSGHITALVVTGKTRSPLFPDIPSLAEVRGGENYPATWFGLFAPADTPKPIIARLA
                     HEVARIVDERDFRQRMYIDRAVEPSGIRLEEFARFIRDERVAAERMVRESGLQLK"
     gene            complement(7328..7531)
                     /locus_tag="VM910_00390"
     CDS             complement(7328..7531)
                     /locus_tag="VM910_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61044.1"
                     /translation="MKTITKTIATRYAMTVGLAGALALTAVSHSFAQIRTGAEAGTTV
                     SQYCAPPDEERPDARRFYCRSGA"
     gene            complement(7645..7812)
                     /locus_tag="VM910_00395"
     CDS             complement(7645..7812)
                     /locus_tag="VM910_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61045.1"
                     /translation="MKTLITALALAALIATTQSAAAAPGDRRDIGQPGQGYEGTYQGY
                     PLSDWYRTDGW"
     gene            complement(8177..8338)
                     /locus_tag="VM910_00400"
     CDS             complement(8177..8338)
                     /locus_tag="VM910_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61046.1"
                     /translation="MTPKLALIISAVVVIAAFVAINSWAAVPKHESNSAAPIAAKGVI
                     YRPALWKVY"
     gene            8604..8831
                     /locus_tag="VM910_00405"
     CDS             8604..8831
                     /locus_tag="VM910_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61047.1"
                     /translation="MYITPYLGDIDASPETKRVLGVALEMTRLSLGLGDNFADGIIAR
                     RIVQLAKAGERNPDLLCKGAVDELCGHLYTD"
BASE COUNT         1762 a         2874 c         2949 g         1744 t
ORIGIN      
        1 ctcctcgctt gccgatcttg ccgcacgcat cgacgaccca gggctcgacg tcagtccgca
       61 ggacgtgctg gtgctgcaga atgccggccc ccatgcgagc gaatgcatgc ctgaggcggg
      121 ttacctcccg atcccgaaga aactcgccca aagcggtgtc aaggacatga tccgagtctc
      181 ggacgcgcgc atgagcggaa ccgcattcgg caccatagtg ctgcatgtga ctccggattc
      241 ggcgtccggt ggcccgctcg gcctcgtccg cagcggcgac cgcatccgcc tttcggtcaa
      301 ggaacgtcgc atcgatctcc tggtcgaaca ggccgaattg aaagcgcggg ccaccgcgat
      361 caagcccacg cagatgaagc ccgagcgcgg ctatgccaag ctctatgcac aggaaattct
      421 cggcgccgat gatggttgcg acttcgcctt cttgcggcca gcccccagtg ggcgctgacg
      481 aatggtcgac ttacgtactg aacgatgtcg cgactatgcc ggcgttatct catcgccgta
      541 agtggcaccg ttttggacgg aatttcatcc gctgcgctag cccactatct tgttgcatgc
      601 cacaggggag gaacaactga tggccgtcga gagcatcttc tattccgtat ccggcatgag
      661 ttgtcgcggg gctctgatct gggacgaagg ggtcaaagcg ccccgtccgc tcttgctgat
      721 ggcgcccaac tggaggggcg tcatcaagcc ggccatcgaa acgggccaga tgctcgcggg
      781 cgagggttat gtcgtcttcg tcgccgacat gttcggcgaa ggtaatggac cggtcgggac
      841 ggaaaaccca atggagtttc tgcggccgtt catgtccgac gtggcaggaa tgcgtcggcg
      901 catcgccgcc gccctcgaca cgctgacgcg ggaagccgac cggcgcgcca tcggcgacat
      961 cagccggcgg gccgcgatgg gcttctgctt tggcggcctc aatgtcctcg atctcgcccg
     1021 caccggcgcg gatgtgcagg cggtcgtctc catgcacggg acgctggcga cgccgcagcc
     1081 cgccaagcgt ggcgacatca aagcggccgt ccttgccgtg cacggcgcca ctgatccggt
     1141 cgcgccaaag gccgaacgcg acgccttcga ggccgagatg acggaggccg gagcgtgctg
     1201 ggggctgctc acattcggcg atgtatacca tgccttcacc gacccgcagg ccaatacggc
     1261 tggcgtcgcc gtatacgacg agcctgccac ccgccatggc tacgcgcttg cccatgcctt
     1321 catcgcagac gcttttgtcg ggcggcggca cggttgctct ctgccgcgcc cggcataata
     1381 atcttaatcc ctatcctacg agcggaggct tcgatgcata ggctgtccat tccgcaatcg
     1441 gtcgtcgatc gcgtcatcga gcgccgcggc cgcgagcatg tctacgaaga tctcgatccg
     1501 gccaagaccg ccctggtcgt tgtcgacatg cagaacgcct tcatgctgcc gggcgttgcg
     1561 cacgcgctct gtccaatggc ggaaaagatc gtgcccaaca tcaaccgtct cgctcaggcg
     1621 gtgcgcgaga ccggcggaac ggtggtttgg atcaaaacca ccttcaagga cgatgcgctg
     1681 caaagctggt cgacctattt cgagatggtg acgccgcaac agagcgccaa acgcatcgaa
     1741 gcgctcacgg ccgacagcaa aggacatcag ctctgggccg atctcgacgt gcgcgccgag
     1801 gacgtcatcg tcgagaagaa tcgcttcagc gcctttattc agggctcgtc gaacctggcc
     1861 gaagtcctgc gcggccgcgg catcgacact attcttatca ccggcacggt gaccaacgtg
     1921 tgctgcgagt cgacggcacg tgacgccatg atgctcaatt tcaagacggt catggtgacc
     1981 gatggcaatg ccgccgtgac cgatgaggac cataacggtt cgctccgcgc gttctatctc
     2041 atgttcggcg atatcgcatc gaccgatatg ctgatcacgt ggctcgcgcg caacgccaag
     2101 aggaatctgg ccgcggccga atagcagtcg ggtacgtcgg cggctgatgg cgccatggag
     2161 ccggcgagac atatctcggc agtaacgccc gagccgacgg agatcgttgc ccgtgcgcgc
     2221 gcgatgatcc ccgcgcttgc ccgccgctcg ctcgaaggtc ggcgccggcg ccgaattccg
     2281 gatgagacca tcgccgacat gcagcgcgcc ggctttttcc gcgtgctgca gcccaagcgt
     2341 tggagcggct atgaaatgga cctccatacc ttctatgaga tccagctcgc gcttgccgag
     2401 ggagatatgt cgaccgcttg gatttatggc gtgtcgggcg tgcatccctg gttcatggcg
     2461 ttgctcgacg atcgcgccgc gcaagaggtc tggcgcagcg atacgtccgc tctgatctgc
     2521 tcgtcgctga tgcccgctgg ccgggccacg ccggccgagg ggggctaccg cctgagcggg
     2581 cgctggagat atgcgagtgg ttgcgagcat tgcgactggg cgctgctcgg cgccatggtc
     2641 gctactgata gcggccgacc gcctgagggg cgaatctttc tcctgccgcg gaaagactat
     2701 gggactatcg acacctggga ggtgtccggc ctgcaggcaa cgggaagctg ggacgtgacc
     2761 gtggaaaacg tgttcgtgcc ggcctatcga tcgcagagca tgctcgacaa tttcctgctg
     2821 aagggccccg gccaggcgct caacagctcg agcctctacc ggctgccgtt tgggcagatt
     2881 ttcgtgcgcg gcatttccac ggctgcgctc ggcgcgttgc agggcatgct caatgcgttc
     2941 ttggactacg gcaaaacccg cgtgacgcgg gcaggcggac gctcggcgga aaatcctttc
     3001 gttcagctgc tttgcgccga aacggtcgcg gccatcgacg agatgacgaa cacgctgcac
     3061 cgcaactttc gaaatttgca cgcttacgcc gggcgaggcg agacgccgcc gctcgaagag
     3121 cgcctgcgct acaagttcca gtcgacggag gtcacggaac gctgcacgct gcttgccgcg
     3181 cgcatcttca aggcgaccgg cgccgcaggc ctggcggagg gtctgccgtt cggtggaatt
     3241 ctggcggacc tcatggccgg gcgtcagcac atttccaatc aatacgaata tgtcggcagc
     3301 agctggggcg gtgtgatgtt cggtcttgag aacaaagatt tgatgctttg aatgtcccct
     3361 cgttgctgcg gttgacgccg caaatcggat tggtctgacc ggccgcaagc tagttctgtc
     3421 gggcccgact acgttgcttg tttcatggcg gcttcgacct cggcgatggc tcgaacgaga
     3481 tcgtcagtgt cccgcttcaa ttgcgcgagg gtcttggcgg cagtgggtag ctgcacgctc
     3541 gagcgggcat ggtagagaag atgcgcttgc ccgatgattg cgggacaacg ctggtccgca
     3601 gcgaccaaga gcgacagaat cgcgctgctg agggacctgt tgtcgtcagc catgatcgtc
     3661 cgccttcgca tccctgccga gcgcgctgcg ccgcgccgcg agcccgatga tggtgagcag
     3721 gaaatcggcg gcctgcggcg gatcggcacg actggtggcg aactccgacg cgccttccgt
     3781 cgcgatgaag caggcgtagc gatgctccca tccgtcgagc tggcggtcga ggaaggcacg
     3841 gcggtccccg tcattcgcca aagttggcag gtgggcgtcg acgcgcgcga tgaagcggct
     3901 tgcgcgatcg gcgggccggc gggcttccga cgtcctcgct tggccttggt gtttcggcgc
     3961 catcttgttt ccgacagcca ttctacaggc tgcggaggaa atggctacat tatgtagcag
     4021 tacttgtcaa ctacattctg tagtaattcc ggattattca gctgcggcgg gaaatgctgt
     4081 ttacccgcgc tgcccattcg atcttgacgt cggcgatggg cttctcggtg gcggaatgca
     4141 ggttgtacag gccctcggcg cggccacgct gcacctgttt gacgaggacg cggccgtcgg
     4201 cgagaccgac cacgcagagc tggccgatca agtcggccgt cattggacgg tgcacatcgt
     4261 cgtagaaaac gtaccagcgg ttgaagaatg gccccagact gtcgccgcgg atctcgaccg
     4321 cgaccgtgtc ctcggcggcg tcggccgggc cgcccacttc gtcaagatct ggctgtgcca
     4381 cgtcgtaaag atgcgccgcc gagccggccc cgacatagcc gaccaccggg accttgggcg
     4441 ccgctggcga ctcgtctgcc cccgtgggga acggttcgcg gccggtcagc agccattccg
     4501 gcgtaacccc gaagcgccgc gcatattttt tcgcgtcctc ggggccatag tcgcggccgc
     4561 cgggactttg cgcgcgctca tgggcgccgt aggtcgacac cggaatgccc atggccttgc
     4621 cggcaaggcg cgccgacttg aagcgcttct cgcgcgccat gcgtagccgc tctccgcgag
     4681 tcgtagccat aggcgcatac taattgcatg ataactacaa ttggtggttg acatcggaga
     4741 caaattgtag ttaaattgtc cccaagaacg atccgggagg cgtgatgttg ccattatcga
     4801 gaggggtcct gccaacgtcg cagcggcggg cctcgccgtg gacgacggag cgcacccggc
     4861 tgttgcggcg acgctggcga caaggcgccc gcgtgcgcga gatcgccgcg gaattgggtc
     4921 acggcatcac ctgcaacgcg gttatctcca agatccatcg cctcgggatt tccgcgcttt
     4981 cgccgtacgg cggcgcaccc ggcagacgct acacagccaa cacgcgtccc gccgataggc
     5041 ccgtacacgc acagcgcgcc gcctggtggt tccggaaagg accgctgccg gcctgggtca
     5101 tcaatgcgag gccctatgtc gagactgtcg gcgccgacgc gcgcatcccg cgccaccagc
     5161 gccgctcgct gctcgaattg aacgatgaaa cctgcagctg gccggtcggc gatccgcggt
     5221 cctctcgctt cttcttcttc tgcggagcgc agccgctttg caacaagccg tactgcgccg
     5281 cgcattgtgc gcgcgcctat cggcggcggg gggagtcgac acgaggcgtc tcggtgctgc
     5341 gtccacgtga cgccaccagc gaagcggaca ggtccatcat gcctagcagc aaaactgcca
     5401 ctcaatgtcg accgaccgga gagaaaaaat gagcgaacga tcaagacaat ccgacatgcg
     5461 ggtgcggaaa tcgataaaga gccgaaagga gccgctggtc gagcgacgcg cgcttccaac
     5521 gcctgaacgt cttcgtcggg ccgggccgga cttcgagcga ggtgataccg gccagattac
     5581 catgcgggac agcccgctgg aacgcgcttt cgcgcgcaac gctctcacgc cagagcaata
     5641 ttcagccggg cagaaatatc gccatcattg gtatcacgcc ggccttgccg accgattgga
     5701 atcaatcgat ctcaaccgcg tctttgcaat cgacctcggt gcctattccg ggatggcgaa
     5761 gacggaaagc caactctttc atcggcaaag ataccgggag gccgcgcaag ccgtcggcaa
     5821 gattggatcg cacgtgttgg aatgggcggt gtgccgcgaa attgcgctcg agcaagtcgg
     5881 ccagacattg ggctggaaca gtcggccaca agcctatgcg gctgctgtcg aacggatgaa
     5941 aacggctttg gatgagctgt gtaagctgtg ggggattggg agttgagccg tcgcacttca
     6001 tcgttcctcg tgggtaacgt gggcgccggc gcccgcacgg ggcaggtcga ttccagcatc
     6061 gcacgcgccg ctcctgcggc tcgagctcga attccgcacg cttatttcag ctgcagcccc
     6121 gactctctga ccatccgttc cgcggccacg cgctcgtcgc gaatgaagcg cgcgaactcc
     6181 tccagtctga tccccgacgg ctcgacggca cgatcgatgt acattcgttg ccggaagtcg
     6241 cgctcatcga caatgcgcgc aacctcatgc gcgagtcgcg cgatgatcgg cttcggcgtg
     6301 tcagccggcg cgaacaagcc gaaccacgtc gcgggataat tttcgccgcc gcggacctcc
     6361 gccagcgagg ggatatcggg aaaaagcggc gagcgcgttt tgccggtgac cacgagggcg
     6421 gtaatgtggc cgctttgcag ctgggggacc atgttggaga gcgccagaat cgcaaccgga
     6481 gtcgacccgg acagcaccgc gttgaccagc tcgttgccgc tcctgaaggg gatgcgcacg
     6541 atatcggcgc cggtctcctg cttgagcttc tccatgaaat gcgcgagcgg aaacgagaac
     6601 gtgcctcagc gtgccgggct tggccttcga caacgccatc agttcgggaa ttgtccgcac
     6661 cttgagcgag cggtcggtga cgagcgccag cgtgttgaaa aacaaaaggc tgatcggctc
     6721 gaaatccttc tcggggtcga acggaaggct cttgaacgcg aactgattgt agaccatcgg
     6781 ctcgctcgaa aggacgcaga tggtgtagcc gtccggcgca gactccgcgc acgcccgcgt
     6841 gccgatgttg agcgcgccgc cgggcctgtt ctccaccacg agcggctgac gcaacgtcct
     6901 cgacagctca tcagcgagcg ctcgggtgaa cacatctccc cctccgccgg cgccgagagg
     6961 aatgatgacc ctgacgggcc gggtgggata gtcctgggcc ggcgcggtcg tacacagggc
     7021 gagcgaagcg acggtgagga tcggggcgat gaaaaatggc ggcacgacgt ccctccgttt
     7081 ttgtttttat tgaggatagg ccgccggtga tcgcggcgca aagccgacgg caacgtgtgg
     7141 cgagatcgaa tcgatttgcc tgcgcgccgc tacccgcgcg caaggcacaa tttatgcgac
     7201 tttgcggcgt tgaggtgtcg caccgcaatc ggcgcgctca caaagcgcca cggatccatc
     7261 cgacccgagg aaatcgcctt ggcagtttgg cgtcggtgcg ctggcacgcc gaaaaaccgt
     7321 tgcccggtca tgccccactg cgacaataga acctgcgcgc gtcgggcctc tcctcgtccg
     7381 ggggtgcaca gtactgagac acggtcgtgc ccgcctctgc tcccgttcgg atttgagcga
     7441 acgaatggct caccgcggtc agcgcaagcg cgccggccag tccgaccgtc atcgcatagc
     7501 gtgtcgctat cgttttcgtg atcgttttca tggccgcacc tcgtgctcaa gcattttcga
     7561 ggttgtgcgg ccggctcgcg agccggccgc ttccgagcgg ctcgctgcga agccccgacg
     7621 ccttcgcggc gacctgattt gactttacca gccgtcagtc ctgtaccaat cgctcagggg
     7681 gtacccctga tacgtgcctt catagccttg tcccggctga ccgatgtcgc ggcgatcgcc
     7741 gggcgccgcc gcggcggact gggtggtcgc aatcaaggcg gccagagcca gagcggtgat
     7801 gagcgtcttc atgggttcat tccatcctgt gacggaccat cgtccgtcgg tgcgtgcagc
     7861 agcgcatagg ctgatgggcc ggcgcctgcg aaatggaaga tgccaccggc ggtgccacag
     7921 tcctgtgatg caggtcacac tgagggatga ttcagaacga tatctgtcaa ttcggcaatt
     7981 tcagagactt agcccaattg aacagctgct cactccatgt atggcgaagg agcggaggca
     8041 agaaattgag aatgaagaaa ggctgccgag gttcaccgcg ccattcagca agcgcaagcg
     8101 gcggaagaat actcggcggc ctgatgacag agaaccgctg cgcccacaag cccccgcccg
     8161 gtcggcgagg gcggacctag tacaccttcc acagggcggg ccggtaaatc actcccttgg
     8221 ctgcgatagg cgcagccgaa ttgctctcat gtttcgggac agccgcccac gagttaatcg
     8281 ccacgaatgc cgcgatcaca acaaccgcgg aaatgatcaa tgcgagtttc ggtgtcatgt
     8341 cttcctctcc tcgcgcttct agcaacagtc ttacaggggg aatcggaaag aaatctgtga
     8401 cggccgtcac gctttggtcg tataggcgga agctatagcc gcggccggac cgtacgccga
     8461 cgcacggtta tgccgcgacg cgcgaagacg cccgcgaggt gcgctagggc gctccgccag
     8521 ctataaacgt cgcttattgt ttcgatgaag ataataaagt tgcacgctaa tggttttttg
     8581 ccaagatagc gtcggaggcc ggcatgtaca tcacgcctta cctaggcgat atcgatgcca
     8641 gtcccgaaac caagcgggtt ctaggtgtcg cgctcgaaat gactcgccta tcgcttgggc
     8701 ttggagacaa tttcgccgac ggaatcatcg ccagacgaat cgtccaactt gccaaggccg
     8761 gcgagcgcaa tcccgatctc ttgtgcaaag gcgcggtcga tgaattatgc gggcatttgt
     8821 acacagacta ataagcaaaa cgctgggtta gcctcgaaat tgaatgcgag cacggtaggg
     8881 acggcctctt cctcacagca agatcgggat cacgctcgat ttgtattctc atgttatgcc
     8941 ggggatacaa gaggacgccg ccgcccgcgt tgacggtgcc ttacaggcgg ccctacagaa
     9001 acgagtggta gtggtagagc agcggttttg taattcggcc gccgctgtgc cggccaatgc
     9061 catcgggtaa cagttggtca gcaatttcgg gcttaagctc gctctctggg ccgcgctttg
     9121 tgccagccgg ttgtcgggca gttgggtagc aaaatggcag caaaggagcc gaagaagttt
     9181 cccaatgaat ggagacgcca gcaccaaaat cccggtcagc ctgcgaatat tagccatcat
     9241 tctccgagcg ctgttcatca gtgcgctggt ggcaataacg gtgcgcgtca gcactcccca
     9301 aagcgaaacc ctttggtccg tacacgaga
//
LOCUS       DATLJT010000019         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2068465,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000019 DATLJT010000000
VERSION     DATLJT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068465"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_00410"
     CDS             complement(<1..230)
                     /locus_tag="VM910_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HVI61048.1"
                     /translation="MTTIDLNSDLGESFGPWEMGNDAAMIELATSVNVACGYHAGDAD
                     IMRKTVEMAKARGVSVGAHPGYRDLHGFGRRP"
     gene            complement(257..1306)
                     /locus_tag="VM910_00415"
     CDS             complement(257..1306)
                     /locus_tag="VM910_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HVI61049.1"
                     /translation="MSKLIVASIGPANSVQDGGRHGAQRYGLPPSGAMDRLALAAANV
                     LVGNDPFAAAIEIGPFTATFKAKDGAVRVALSGAPRSADVAGRPVAVDMSMTIADGES
                     LTLGFAKGGAFSYLAIEGGIAGEPMFGSLAVNARAGLGSPYPRPLHAGDELQVNAASG
                     GTERRIELPAPATGPIRVIFGPQDDEFSDDARALFLECEWKISPTSDRMGYRLEGPVI
                     KHLDGHNIVSDGTVDGSIQVPGNGAPIVLMPDRGTSGGYPKIATVLSVDLGRMAQIPA
                     GTAFRFKAVSMAQAQAEARKFADLLRSLPDRLRAIDATALNIDALHDVNVAGSAVNAI
                     DVDTWHAEADVLTPD"
     gene            complement(1303..2034)
                     /gene="pxpB"
                     /locus_tag="VM910_00420"
     CDS             complement(1303..2034)
                     /gene="pxpB"
                     /locus_tag="VM910_00420"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HVI61050.1"
                     /translation="MAATLAPPRLLPSGDRAVTVEFARTIDDAANQRVLALDRALHET
                     PIEGVTETVPTYRSLLVHYDPVRIGFDELGTKIVALALQPSPPTEKAKHWRIPVAYGG
                     ENGIDLGDVAKTLQTTPDDIVARHVAGQYRVAMIGFTPGWSYLSGLDKFLHISRRQNP
                     RLLTPAGTISIGGIQTGVQCLAGPSGWHLLGRTAVRTFQLHRDPIFLLEPGDHVTFFA
                     VDAKTFWEQDRAAEAGEIVAELMSA"
     gene            2256..2954
                     /locus_tag="VM910_00425"
     CDS             2256..2954
                     /locus_tag="VM910_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HVI61051.1"
                     /translation="MSLSKDAIATLSHVSTATITTVLLKKGLRNVWMRGARPLKPGQK
                     RLVGPAFTLRFVPAREDLATPESWSSPISTRTAIEAMPNGCIAVVDAMGITDAGIFGD
                     ILCARMVKRGVTALITDGVVRDVEGVLGTGLPVWCNGYAAPPSVAGLTFVGWDQPIGC
                     GGVAVFPNDIVVADQDGAVLIPQTQLDHVLSEGPEQERMEGWIVNEVNNGAVLPGLYP
                     MNAETKARYAASKK"
     gene            2991..3395
                     /locus_tag="VM910_00430"
     CDS             2991..3395
                     /locus_tag="VM910_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI61052.1"
                     /translation="MDIYLAGSRPTRRAPKESFTGTVWQDPIITPQAPARVVAGRVAF
                     EPGARTAWHTHPLGQTLYVIAGVGRVQAKGGPIREIKAGDVVWIPPGEKHWHGAAPTT
                     GMVHIAMQESLDGNHVNWMEHVTDAEYSAAVG"
     gene            complement(3551..>4284)
                     /locus_tag="VM910_00435"
     CDS             complement(3551..>4284)
                     /locus_tag="VM910_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI61053.1"
                     /translation="IDPDRGTVSIPGVYDNTGRKSKRARDQDSKTKQSPREAKVSPET
                     PPPPAAPAPAAAAPATTAAPAASPPAVATAPAPPPATTTTTAPDTATANAPPTDTAAL
                     PPPPPPPLPSQDVAAPPPAPTVATTAPVPTPQPSVPAPAIRDPNSPIGVWLTEEKEGK
                     VRIEPCGPNLCGYSVDAKTNLNGEQVLINMKPAQALKWAGRIHDPNTGSNYDSTIALK
                     GDTLRVQGCAFGGMFCGGQTWTRVN"
BASE COUNT          744 a         1341 c         1413 g          786 t
ORIGIN      
        1 aacggtctgc ggccgaagcc gtgaagatcg cgatatcccg ggtgcgcgcc gaccgatacg
       61 ccgcgcgcct tcgccatttc gaccgtcttg cgcatgatgt cggcatcgcc ggcatggtaa
      121 ccgcaggcga cgttgacgga cgtagcaagc tcgatcatcg cggcgtcatt gcccatttcc
      181 catgggccaa agctttcgcc gagatcgctg ttgagatcga ttgtggtcat gggttttccc
      241 gcttcgttga cgtgaactag tccggtgtca ggacgtctgc ctctgcatgc caggtatcta
      301 catcgattgc gttgaccgcg ctgccggcaa cattaacatc atgcagcgcg tcgatgttga
      361 gcgccgttgc atcgatggcg cgcaggcggt ccggcagact gcgcaacaga tccgcgaatt
      421 tcctggcttc ggcttgcgcc tgcgccatgc tgacggcctt gaagcggaac gcggttccgg
      481 ccggaatctg cgccatccgc ccgagatcga ccgagagcac ggttgcgatc ttgggatagc
      541 cgccgctggt gccgcggtcc ggcatcaaga cgatcggcgc gccattaccg ggcacctgga
      601 tgctgccgtc cacggtgccg tcggagacga tgttgtggcc gtcgagatgc ttgatcacgg
      661 gaccttccag gcgatagccc atgcgatcgc tggtcggcga aatcttccat tcgcactcca
      721 ggaacaacgc cctggcgtcg tcggaaaact catcgtcctg cggaccgaag atcacgcgga
      781 tcggaccggt cgccggagcg ggaagctcga tccgacgttc cgtcccgccg cttgcggcgt
      841 taacctgcag ctcatcgccc gcgtggagcg gccgcgggta ggggctgccg agaccggcgc
      901 gggcattgac tgcgaggctg ccaaacatcg gctcgcccgc gataccgcct tcgatcgcga
      961 gatagctgaa cgcgccgccc ttggcaaagc cgagcgtcaa gctttcaccg tccgcaatcg
     1021 tcatcgacat atccacggca accggccggc ccgcgacgtc cgcactgcgg ggggcgcccg
     1081 acagggctac acgcaccgcg ccgtccttcg ccttaaatgt cgcggtgaat gggccgattt
     1141 caatggcagc ggcgaacggg tcgtttccga ccagcacgtt tgccgccgca agcgccagcc
     1201 ggtccatcgc gccgctcggc ggcagcccgt aacgttgcgc gccgtggcgc ccgccgtcct
     1261 ggacggagtt cgcgggcccg atcgatgcga cgatcagctt gctcatgcgg acatcagttc
     1321 ggcgacgatc tcgccggcct cggcagcacg atcttgttcc cagaaggtct ttgcatcgac
     1381 cgcaaagaac gtcacgtggt cgccgggctc aagcagaaag atcggatcac gatgcagttg
     1441 gaaagtcctg accgccgtgc ggcccaggag atgccagccg ctggggccgg caagacactg
     1501 gacgccggtc tggatgccgc cgatcgaaat cgtgccggcc ggcgtgagca gccgcgggtt
     1561 ttgccggcgg gatatgtgca ggaacttatc caggccgctc aaataggacc agcccggcgt
     1621 aaagccgatc atggcgacgc ggtattgacc cgcgacatgc cgcgccacga tgtcatctgg
     1681 cgtggtttgc aacgtcttgg cgacatcccc cagatcgatg ccgttctccc cgccgtaggc
     1741 gacgggaata cgccaatgct ttgccttctc ggtcggcggg gagggctgca gcgcaagcgc
     1801 aacgatcttc gtgccgagtt catcaaatcc gatcctgacc ggatcgtaat gcacgagcag
     1861 cgagcgatag gtcggaacgg tttcggtcac gccttcgatc ggcgtttcat gaagcgcgcg
     1921 atcgagcgca agcacccgct ggttggcggc gtcatcgatg gtgcgggcaa attccaccgt
     1981 gacggcacgg tcgccactcg gcaaaagacg gggtggggca agcgtcgcag ccatggcctc
     2041 acagcggatc ggcgggtttt gcgacctgta gcctgtttcg gtgcgaagtg gaattcgctt
     2101 ccccgcaaat cacccgtaaa ttcaataaat taatcgaatg tggcgtaata gggcggcttt
     2161 gtcccggcaa agctgcctcg gcacttgacg caagggacgc acctcgcaag atgcccctgc
     2221 agtttttttg cgcgcgaacg gagccaaatc ccaaaatgtc cctgtcaaaa gacgcgatcg
     2281 ccaccctctc tcacgtctcg accgcaacga tcaccacggt tctcctcaag aagggactgc
     2341 gaaacgtctg gatgcgcggc gctcgaccgc tgaaacccgg tcagaagcgg ctggtcgggc
     2401 cggcattcac cctgcgcttt gttcccgcgc gcgaagatct ggcgacgccg gagtcgtggt
     2461 cgtcgccgat ctcgacgcgc accgcgatcg aagcgatgcc gaacggctgc attgccgtgg
     2521 tcgatgccat gggcatcacc gatgccggta tcttcggcga cattctctgc gcgcgcatgg
     2581 tcaagcgcgg cgtcaccgca ctgatcacag atggcgtcgt gcgcgatgtc gagggtgtcc
     2641 tcggcaccgg tcttccggtc tggtgcaacg gttacgcggc gccgccgtcc gtggcgggat
     2701 tgacttttgt cggctgggac cagccgatcg gttgcggggg cgttgcggtg tttcccaacg
     2761 acatcgtggt tgccgatcag gacggtgcgg tgttgatccc acaaacgcaa ctcgaccatg
     2821 tgctgtccga ggggccggag caggaacgga tggaaggctg gatcgtcaat gaggtgaaca
     2881 atggcgcagt gctgccgggt ctctatccga tgaacgccga aaccaaggcg cgctacgcgg
     2941 cttcgaagaa atagcggggc aacccgcgct cagcccaagg gagaacaggc atggacatct
     3001 atctcgctgg atcgcggccg acgcgtcgtg cgcccaaaga aagctttacc ggcaccgtct
     3061 ggcaggaccc gatcattaca ccacaggcgc ccgcgcgcgt ggtggcgggc cgggtcgcct
     3121 tcgagcccgg cgcgcggacc gcgtggcata cccatccgct cggccagacg ctctatgtga
     3181 ttgcgggcgt cgggcgggtc caggccaagg gcggtccgat ccgcgaaatc aaggccggtg
     3241 acgtcgtctg gattccacct ggcgaaaaac attggcatgg ggccgcgccg acgaccggca
     3301 tggtgcacat cgccatgcag gaatcgctcg acggaaacca cgtcaactgg atggagcacg
     3361 tcaccgacgc ggaatattca gccgccgtcg gataagcccg cccgtcgagg tgtgttgcgg
     3421 tcaaatctcc tgcgcggtgc aggctccggt tcgaacattg tgcgcgtggc aggcgacctg
     3481 acgcagccac aaaagaaaaa ccccgtctcc ttgagacggg gtttttcttg cggacgcgtc
     3541 aatccgactg tcagttcaca cgggtccagg tttggccgcc gcagaacatg ccgccaaaag
     3601 cgcaaccctg cacgcgcagc gtatcgccct tcagcgcgat ggtggaatca taattgctgc
     3661 cggtgtttgg atcgtggatc cggccggccc atttcagagc ctgcgcgggc ttcatgttga
     3721 tcagaacctg ctcgccattg aggttggtct tggcgtcgac cgaatagccg cagaggttcg
     3781 gtccacacgg ctcgatacgg accttgccct ccttctcttc cgtcaaccag acgccgatcg
     3841 gcgaattggg gtccctgatc gccggcgcag gtaccgacgg ctgaggagtt ggcaccggtg
     3901 cggtggtggc aaccgtcggc gcgggaggcg gcgccgccac atcctgagaa ggcagcggtg
     3961 ggggcggggg cggcggcaaa gccgcagtgt ctgtcggcgg agcgttggcc gtcgcagtgt
     4021 ccggcgctgt cgtggtggtc gtggccggcg gaggggccgg ggccgttgcg accgcaggcg
     4081 gcgacgcggc gggtgctgcc gttgttgccg gcgcggcggc tgccggcgca ggagctgcgg
     4141 gtggaggcgg tgtctccggg ctgaccttcg cttctcgcgg gctctgcttg gtcttgctgt
     4201 cctggtcgcg tgcgcgcttc gatttccgtc cggtattgtc gtagacgccg gggatcgaaa
     4261 ccgttccgcg gtccgggtcg atgc
//
LOCUS       DATLJT010000020         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_779, whole
            genome shotgun sequence.
ACCESSION   DATLJT010000020 DATLJT010000000
VERSION     DATLJT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..851
                     /gene="lepB"
                     /locus_tag="VM910_00440"
     CDS             81..851
                     /gene="lepB"
                     /locus_tag="VM910_00440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVI61054.1"
                     /translation="MKANSARTGITKRKSSGGVVDTIKTVVYAILIALVVRTVAVEPF
                     NIPSGSMVPTLLIGDYLFVSKFSYGYSRYSLPLGLPLFSGRIFFHSPERGDVTVFKLP
                     TDNSTDYIKRVIGLPGDHIQMKSGILYINDQPCLRKRIEDFLYQEGNGAVIPLAQYIE
                     TLPNGVQHRIIKMGDNGPLDNTQIYDVPPGEYFMMGDNRDNSQDSRVLSAVGYVPAEN
                     LIGRAEFIFFSTDGSARLWEFWRWPFAIRYSRLFRGVN"
     gene            876..>1525
                     /gene="rnc"
                     /locus_tag="VM910_00445"
     CDS             876..>1525
                     /gene="rnc"
                     /locus_tag="VM910_00445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVI61055.1"
                     /translation="MSAEELGHIIGYEFACRELLEEALTHPSALVPERRRYRRPIPVK
                     RDFERLEFLGDRVLGLVVADYLWRRFEGEPEGDLTRRHTHFVRREALVRVAETIGLGR
                     YIVLSRAEATAGAAGNPGILADACEALIAAIYLDGGFEAASTFVRRFWEPLIEEMQEP
                     PRDPKTALQEWVQARGLPLPAYELVATSGPDHAPLFTVTASVAGGDPASATASSKRT"
BASE COUNT          290 a          459 c          445 g          331 t
ORIGIN      
        1 gctttttcgg caggcgcgcc cttgactcgc cgcatcgcga tgggcttgat cctgcgccaa
       61 ccaggccctc aaggcaatcc atgaaagcca attctgcgcg cactggcatc accaagagaa
      121 aatcctccgg tggcgtcgtc gacactataa agactgtcgt ttacgccatt ctaattgctt
      181 tggttgtccg cacggtcgct gttgagccgt tcaatattcc atccggctcg atggtgccga
      241 cgttgctgat aggcgactat ctattcgttt ctaagttttc ctacggatat agccgctact
      301 cgttgcccct gggattgccg ctgttctcgg ggcggatttt ctttcactca cccgagcgcg
      361 gcgacgtgac ggtgtttaag ctgccgacag acaactctac ggattatatc aagcgggtga
      421 tcgggctccc cggcgatcat atccagatga agagcggtat cctctacatc aacgaccagc
      481 catgtctgcg caagcgcatc gaggacttcc tttatcagga aggtaatggc gcggtaatac
      541 cgctcgccca atatatcgaa acgctgccaa acggcgtaca acatcggatc attaagatgg
      601 gcgacaacgg cccgctcgac aacactcaga tctatgacgt accgcccggc gaatacttca
      661 tgatgggtga caatcgcgac aactcgcagg atagccgtgt gctttctgcc gtcggctacg
      721 tcccagccga gaacctgatc ggacgcgctg agtttatctt tttttcgacc gacggctcgg
      781 cgcgtctttg ggagttctgg agatggccgt tcgcgattcg ctatagccgg ctgtttcgcg
      841 gcgtaaacta gcgcaaacgt cggcagagag ggattttgtc ggcggaggag ctcgggcaca
      901 tcatcggtta cgagttcgct tgccgcgaac tcctcgagga ggcactgacc catccgagcg
      961 cgctagtccc cgaacgtcgt cgctatcggc gtccgatccc cgtcaagcgc gatttcgagc
     1021 gccttgaatt tctcggcgat cgcgttcttg gtctcgtcgt ggccgattat ctgtggcgac
     1081 gctttgaagg tgagcccgag ggcgatctta cacgccggca tacccacttc gtgcgccgcg
     1141 aggcgcttgt ccgggtcgcc gaaacgatcg gcctgggacg gtatatcgtt ctatcgcggg
     1201 ccgaggcaac cgccggggcc gcaggcaacc ccgggattct cgccgatgcc tgcgaagcct
     1261 tgattgcggc aatctatctc gacggcgggt ttgaggccgc gtcaaccttt gtccgacggt
     1321 tctgggagcc gctgattgaa gagatgcagg agccgccgcg cgatccgaag acagcgttgc
     1381 aagaatgggt tcaagcgcgc ggtctgcccc ttcccgcgta cgaactcgtc gcgacaagcg
     1441 ggccggacca cgcgccgctc ttcactgtaa cggcgagtgt ggccgggggc gatcccgcca
     1501 gcgcaaccgc ctcgtcgaag cgaac
//
LOCUS       DATLJT010000021         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1149561,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000021 DATLJT010000000
VERSION     DATLJT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149561"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..521)
                     /locus_tag="VM910_00450"
                     /pseudo
     CDS             complement(185..521)
                     /locus_tag="VM910_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024997.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            517..724
                     /locus_tag="VM910_00455"
                     /pseudo
     CDS             517..724
                     /locus_tag="VM910_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934119.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1702..>2811)
                     /locus_tag="VM910_00460"
     CDS             complement(1702..>2811)
                     /locus_tag="VM910_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI61056.1"
                     /translation="AIFVSLELSRSTWLITSLSPGAREKMSKHSIRAGDVAGLLVRLA
                     QLKEKARARTGRDFPIIAIQEAGLDGFWIHRVLQSEGIESHVVDAASILTSRRRRRAK
                     TDGIDGETLVRTLLAYKRGEPRVCAMVKAPTPEEEDRRRICRERKTLVGERVEHVNRI
                     KGLLFAQGVSNYEPLKRNRRARLEELKTGDGRPLPAHLKAQIGRELDRLELVLPQLKA
                     VEAERDALLKPVSEGCPTPAAMLAQLKGMGPEFTAILWSEGLFRSFSNRRQLAAYAGL
                     APTPWQSGSVAHEQGVSKAGNPRLRTTMIQLAWLWLRHQPTSALSVWFRERVQSKGGK
                     IRKTTIVALARKLLVALWKYSTSGVVIEGAAMKTI"
BASE COUNT          480 a          942 c          810 g          579 t
ORIGIN      
        1 gagacaagtc gctatattcc ttcgcgacga agcgggaagc ccaagctgac gcagacaagt
       61 ttgtccaaag gctcaattcc gtttggcgac ctcgccaagg gggcgtgtca aagtaacgag
      121 gctcgtcgat atgccgcgcg accgccgtcc gcgagcggca atctacgccg cgagagcgcg
      181 ccactcagcc aggttgcctc gtgacgagat ggcgctcctg attgaattgg ttgtgcacct
      241 gggcatgaac tgagctgaac ttctgcagcg tcttcatgct tcgaaagcgc tgcatcgccc
      301 gttcccgtcg ccgaaacggc tgatgggaat tctctgcgcg attgttgagc cgaccgccga
      361 cctcgtgccc gctcggcaac accgatctcg ttcatcgccg ccgaataggc tcgaagccca
      421 tcggtgacga tgctgccagg agccccgtat ttcttcatga tccgcttgag aagcttgagc
      481 gccgcagcct tgtcccgtcg tgcggtgact accgcctcca agacctcgcc ctcatcaacc
      541 ggttcgacgg cgcgagcgca agatgcagcg cttcaaatcg ccaggatcag cccagcgctt
      601 cctctccact cacgccgccg tgcagaacac tttcaatgtc cagcgccatc tcacatcccg
      661 caccacgttc cgagtcctcc gagagaaggc gttccaaacg tggcgagcgg ccacagcggc
      721 ataaggagaa attgaacctc cattaccttc tgacgccaga actaagttgt tcgcggtagg
      781 gacgtccatt gctggacgcc ccccgcacag atccggacga gcccgattcg agcatccggc
      841 tcctacctcg ggtgtgtgac ggcgaagcgc cgctcaggcg cttgccgtac gcggcccagc
      901 gcctgggtca cgcgtgcccg gctctgggtc cggagcgtgc tttgctggtc cgcgttcccc
      961 ttggtccccg cccttggctc caccgcctcc gccgccgggg tcccggcttt gttcgacggc
     1021 ttcacagcta ctatggcgga gtcagacttc tcctgatcgt gcatcggcgg ctacggctcc
     1081 tcaccttccc gccgcggacc atccggccca ggagggccta tggccgacca aaagatctcc
     1141 cggttcccgt acaagaagct tgcggacatg ccagggtctt cgaccacgcc gggccgtcca
     1201 gacgctcgcg ctggcgcgtc tgaacatgtc gccttccgct acacagacag cgtcggcgcc
     1261 cgggattagt tttctatcgc ggctcaatgg ctggcctgtc cgttcccctg ccgacgcttc
     1321 gccgatgtcc tcacggatat ctgcgcacgg cttggggccg acgtggttcg ctactccttc
     1381 atcgcagagg actcacatgg gcgttttcgg gtcaaggggt caggttcatc ggcatatcaa
     1441 aaactccggt tgctgtttcg gtatccaagg tctgagccct atttcatgac ctggttggtt
     1501 ccgcaccaga tcaaactcac atccggtcgc cattcaaagc agctgcaccg atatcccgcg
     1561 ttcggcggat ggggctcagg aggtcgagac cattctttca ctcggctgtt taagccagtc
     1621 ctctgctcgg ttccgatcgc ctggatccgt caggaccgat taggtcccgg ggattggggg
     1681 attgtgttgg agcgtctgcg ctcagatggt cttcatcgcg gcgccctcga tgacgacgcc
     1741 gctggttgaa tatttccaca acgcgacaag gagcttgcga gccagcgcaa cgatcgtggt
     1801 cttgcgtatc ttaccgcctt tgctctggac gcgctctcgg aaccagacgc tcagcgccga
     1861 ggttggctgg tgtcgcagcc acagccacgc gagctgtatc atcgtcgttc gcagccgcgg
     1921 attgccggcc ttggagaccc cctgttcgtg agcaaccgat ccgctctgcc agggtgtcgg
     1981 cgccagccca gcgtaggccg ccagttgcct tcggttgctg aagctgcgga acagcccttc
     2041 agaccagagt attgccgtaa attccggtcc cattcccttc agctgcgcca gcatcgccgc
     2101 aggcgttggg cacccttcgc tcaccggctt gagtaaggca tcccgttccg cctcgaccgc
     2161 cttcagctgc ggcaggacga gttcgaggcg gtcgagttcg cggccgatct gcgctttcag
     2221 gtgcgccggc aaggggcggc catcgcccgt cttcaattct tccagccgcg cccggcggtt
     2281 acgcttcaac ggctcataat tagagacgcc ttgggcgaaa agcagcccct tgatccggtt
     2341 gacgtgttcc actcgctcgc caacgagcgt cttgcgctcc cggcagatcc ggcggcggtc
     2401 ttcctcttcg ggtgtcggcg ccttgaccat cgcgcacacc cgcggctccc cgcgtttgta
     2461 tgctaagagc gtgcggacca gcgtctcccc atcgatcccg tcggtcttcg ctcggcgccg
     2521 gcggcgcgag gtcaaaatcg acgcggcatc gacaacgtgg ctttcgattc cttcgctctg
     2581 cagaacgcgg tggatccaga agccgtccaa tccggcctct tggatggcga tgatgggaaa
     2641 gtcccgtccc gtccgcgctc gcgctttctc cttgagctgg gccaaccgca cgagcaagcc
     2701 cgccacatcg ccggcccgaa tcgagtgctt cgacatcttc tccctggcgc ctggcgacaa
     2761 agatgtgatc agccaggtag atcggctgag ttccaaagac acaaaaattg c
//
LOCUS       DATLJT010000022         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2757779,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000022 DATLJT010000000
VERSION     DATLJT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757779"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VM910_00465"
     CDS             <1..900
                     /locus_tag="VM910_00465"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI61057.1"
                     /translation="IGASPHGQGLATTLAQLVADAIGVVPASVRVVHGDTDRTPYGWG
                     TFASRSLVISGGAATLAARKMREKLLTIAGDLLEASRDDIVLEDGKARVTGTDRSVTM
                     AVLARAAYHQSHRFKDLSPGLAESAHYDPPGTFSNACHVAIVDVDVETGGVRLERFLA
                     VEDAGRIINPLIADGQVHGGIAQGIGNALLEEIVYDALGNVLTATLADYLPPTVREVP
                     PIELRHLETPAAGSVTGAKGLGEGGAIGAPAAILNAVNDALSPFGVSIDEMPATPQRI
                     RAALRAAVIGDGRSGIRDEVRDS"
     gene            complement(922..1377)
                     /locus_tag="VM910_00470"
     CDS             complement(922..1377)
                     /locus_tag="VM910_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HVI61058.1"
                     /translation="MTDQPQRRATRPSDLLEPVRLAKVVRGGTWTDYLVLFLRLMAGV
                     SLIKGLYHWIQVCGIGAAPGQGFEAHTIAWQTTTVFFAVLDLVAAVGLWLAAPWGAVL
                     WLASVVSMSAVEVFFPQVYGGSLFVVFVETTLLGVYLFLAIVSARERAT"
     gene            1540..>2444
                     /gene="hemB"
                     /locus_tag="VM910_00475"
     CDS             1540..>2444
                     /gene="hemB"
                     /locus_tag="VM910_00475"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVI61059.1"
                     /translation="MAIKFGRPLERSRFAPAPAEVKAERLDLAMRPRRNRRSEWSRRM
                     VRENVLTADDLIWPLFIVDGTQVRAPVESMPDVERLSVDEAIRAAEQAAALAIPCVAL
                     FPYTDPDRRDDAGSEALNPDNLVCRAIRAIKKAVPEIGVLCDVALDPYTSHGHDGLLR
                     DGVILNDETVAVLVRQALVEAEAGCDIISPSDMMDGRVGAIRSGLDAAGFADVQIMAY
                     AAKYASAFYGPFRDAIGSNATLIGDKRTYQMDPANTDEALREVELDIAEGADMVMVKP
                     GLPYLDVLQRVKDAFGMPTFAYQVSG"
BASE COUNT          475 a          773 c          778 g          418 t
ORIGIN      
        1 atcggcgcga gcccgcacgg ccaaggcctg gcaacgacgc tggcgcagct tgtcgccgac
       61 gcgatcggag tcgtgccagc gagtgttcgc gtggtgcatg gcgataccga ccgcacaccc
      121 tatggctggg ggacatttgc cagccggtcg ctcgtcatca gtggtggcgc tgcgacgctg
      181 gcggcgcgca agatgcgcga aaagctgctc accattgccg gcgatctgtt ggaggcctcg
      241 cgcgacgaca ttgtgctcga agatggcaag gctcgcgtca ccgggacgga tcggagcgta
      301 acaatggcgg tgctggcgcg cgcggcctac caccagtcgc accgcttcaa ggatctgagt
      361 ccgggtcttg ccgagagcgc gcactacgat ccgcccggca ctttttccaa tgcctgtcat
      421 gtcgccatcg ttgacgttga cgtggagacc ggaggcgtcc ggctggagcg ctttcttgcg
      481 gtcgaggatg ccggccgcat catcaaccca ctgatagcag acggccaggt gcacggcggc
      541 atcgctcaag gcatcggcaa cgcgcttttg gaagagatcg tctatgacgc gctcggcaac
      601 gtattgaccg cgacgcttgc cgactatttg ccgccaaccg tgcgtgaagt tccgccgatc
      661 gagttgcggc acctcgaaac gccggcggcg ggctcggtga ccggcgccaa aggcctcggc
      721 gagggcggcg cgattggggc gcctgccgcg atcctcaatg cggtgaacga tgcgctctcg
      781 cctttcgggg tctcgatcga cgagatgccg gcaacgccgc agcgcatcag agctgccctg
      841 cgggccgcag tgatcggtga tgggcgatca gggatcaggg atgaggtaag agactcctga
      901 ttccagatca ctgactcatg gttacgtcgc gcgctcgcgc gctgaaacga tcgcgagaaa
      961 caaataaacg ccgagcagcg tcgtctcgac aaaaaccaca aaaaggctgc cgccgtagac
     1021 ctgcggaaag aacacctcca ccgccgacat cgagaccacc gaggcaagcc agaggaccgc
     1081 gccccacggc gcggcgagcc acagcccaac cgccgccacc aaatccagca ctgcgaaaaa
     1141 caccgtggtg gtctgccagg cgatcgtgtg cgcctcgaag ccttgtcccg gcgccgcgcc
     1201 gatgccgcag acctggatcc agtgataaag ccccttgatg agggacaccc cggccatgag
     1261 ccgcagaaac aggaccaaat aatcggtcca ggtcccgccg cggacgactt ttgccagccg
     1321 caccggctcg agcaggtcgc tgggcctcgt cgcgcggcgc tgcggttgat cggtcatgat
     1381 ccttgccttt aagggatcaa ggggcggatt gatggcgaac aggcctgcag atcgatacgg
     1441 cactttggca gcgcgtcaaa gcccaggaat cggcctcaaa ccaatgcctt gtggcagatc
     1501 ggcccgggac gagataaaac aggggtaagg agacctctca tggcgatcaa attcggccgt
     1561 ccgctcgaaa ggagccgctt tgccccggcg ccggccgagg tcaaggctga acggctcgat
     1621 ctcgcgatgc ggccccgccg caaccgacgc agcgagtggt cgcgccgcat ggtgcgcgaa
     1681 aacgtgctca ccgccgacga cctgatctgg ccgttgttca tcgtcgatgg aacgcaggtg
     1741 cgcgcaccgg tcgagtcgat gccggacgtg gagcgcctat cggttgacga agcgattcgt
     1801 gccgctgaac aggcggccgc gctggcaatt ccgtgtgtgg ctttgttccc gtataccgat
     1861 ccggatcgcc gcgacgatgc cggttcggaa gcgctcaatc ccgacaatct cgtctgccgc
     1921 gccattcgcg ccatcaaaaa agcggtgccg gaaatcggcg tgttatgcga cgtggcgctc
     1981 gatccctata ccagccatgg ccacgacggc cttctgcgcg acggcgtgat cctcaatgac
     2041 gagaccgtcg ccgttctggt ccgtcaagcg ctggtggagg cggaagcggg ctgcgacatc
     2101 atctcgccgt cggacatgat ggacggccga gtcggcgcca ttcgcagcgg actcgatgcc
     2161 gccggctttg ccgacgttca aatcatggcc tatgcagcca aatatgcctc cgccttctat
     2221 gggccgtttc gcgacgccat cggttcgaac gcaactctga tcggcgataa gcggacttac
     2281 cagatggatc cggccaatac cgacgaggcc ttgcgcgagg tggaactcga tatcgccgaa
     2341 ggcgccgata tggtgatggt gaaacccggt ttgccctatc tcgacgtttt gcaacgtgtg
     2401 aaagacgcat tcgggatgcc caccttcgcc taccaagtct ccgg
//
LOCUS       DATLJT010000023         5572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_919944,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000023 DATLJT010000000
VERSION     DATLJT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919944"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..957)
                     /locus_tag="VM910_00480"
     CDS             complement(127..957)
                     /locus_tag="VM910_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HVI61060.1"
                     /translation="MIGHRFRLLCAAGLLGAALGAPPASVAAAEPYDPWPGLVQDIFN
                     NRPMNDGSDVIAIEMPYRAEDAAIVPVTLRTKLSSDDVRRVLAITLVIDQNPAPMAAK
                     FELGPDARVSEISTRVRVNSYTNVHAVAELSDGKLYMIKTYVKASGGCSAPAAKNAEE
                     AKNRLGQMKYREFAKDDQSPTNGTREAQIMIGHPNNSGLQRDQLTLLYIPAFFINELR
                     LWQDNSPVLSVESGISISEDPNIRFTYVSNGAKLFRAEARDTAGHVFQKDWKVDDSGS
                     "
     gene            complement(1014..1943)
                     /locus_tag="VM910_00485"
     CDS             complement(1014..1943)
                     /locus_tag="VM910_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HVI61061.1"
                     /translation="MGFHRVLLCIVLALAAASISARAEQQALPVTEIAPGVFVHVGDV
                     ALMTRENEGAIANVGFVVGDNAVAVIDTGGSVREGRQLLAAIRAKTDKPIRYVVNTHG
                     HPDHAFGNAAFLAEGAIFVGHKNLPRALSMRGQFYLDAFRGIMGDELIDEVRLVPPTL
                     LVDDTLKLDLGSRTLTLRAWPTAHSDSDLTIFDETSKTLFAGDLLFLTHVPVVDGSLG
                     GWMQIMGNLSALPAERVIPGHGSVSDWPAALADERRYLDTLATDVRISVKRGTPISAA
                     AETAAASERKNWRLFDDYNARNATAAYSQIEWE"
     gene            2163..3050
                     /locus_tag="VM910_00490"
     CDS             2163..3050
                     /locus_tag="VM910_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI61062.1"
                     /translation="MKTAVIERCLLISLLAIPASFVRGDVARAQANNEGLDLSIELVD
                     PKVLRVCADPRNLPFSNEKGEGFENKLAEFFADKLQKKLDYVYYPQATGFVRMTLGAH
                     RCDVIMGFPQGDELVQGTNPYYRTAYALIAKPGSGLEEVATLEDQRLKGKRIGIVAGT
                     PPATYMAANGLMSNAKPYPLMIDTRVDSSAEAMINDLNKGEIEAGVLWGPMAGFYARK
                     SNPPLHVTPLVKETSGPRLVYRIGMGVRSADQNWKRLLNRLIQENQPAINKILLDFGV
                     PLLDENDQPINAETATKSP"
     gene            3047..3622
                     /locus_tag="VM910_00495"
     CDS             3047..3622
                     /locus_tag="VM910_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HVI61063.1"
                     /translation="MIRRAAALFVLTFAFATAAPAQDIVPEPADYRTEDYRAPVPATL
                     AGARVLTTREAEAIWRARSGIFIDVLPRAPKPPNLPAGTVWRDKPRLNIPGSVWLPDT
                     GYGRLAPPTEYYLRQGLARASGRNQSALIVVYCQADCWMSWNAAKRILSYGYSNVAWY
                     PDGTDGWERADLPTTEAQPEPRPADAELPPG"
     gene            complement(3720..4331)
                     /locus_tag="VM910_00500"
     CDS             complement(3720..4331)
                     /locus_tag="VM910_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61064.1"
                     /translation="MRWPIIFAALLAIAISTRPGFAADPRYPDWPCAQAKVPEISLPA
                     VWAGPSLDDIKDKWKDDAKISALVAKLAARRTPLDEAEKTIKDYLAGAADKVTAGKLL
                     FAGLFDTLNEQRSSVMNGLERVMRKQRDAADKIRSDTLALQALQDATSRDPAKVDELG
                     NQLVWETRIFEDRRRVMKFVCEVPTTIDQRLFALGRTIQQEME"
     gene            complement(4328..5176)
                     /locus_tag="VM910_00505"
     CDS             complement(4328..5176)
                     /locus_tag="VM910_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61065.1"
                     /translation="MSSTIVDQRRRGFALTEYATCLSGIVWREALRFLHQRERFVSAL
                     VRPLVWLFIFAAGFRQVLGISIIPPYETYILYEVYIAPGLMAMIQLFNGMQSSLSMVY
                     DREMGNMRTLLVSPLPRWYLLFCKLVAGTAVSLLQVYAFLLIAWFWDITPPPIGYLTV
                     LPALILSGLMLGALGMLISSGIKQLENFAGVMNFVIFPMFFASSALYPLWRVQEGSPW
                     LYYVCEANPFTHAVELIRFALYGKINWISLAVVAGCTTIFMIGAILAYDPARGFSRRG
                     PAGGET"
     gene            complement(5173..>5572)
                     /locus_tag="VM910_00510"
     CDS             complement(5173..>5572)
                     /locus_tag="VM910_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61066.1"
                     /translation="VLARLGLADRAGSKVRDLSGGQMRRLEIARALLHRPRLLLLDEA
                     TVGLDVKARADILAHVRQLVGDQGIGVLWATHLFDEIVPSDDLVILHQGRVVARGDGA
                     TVLKEAGAADVNTAFMRLTGTAALSGSLPP"
BASE COUNT         1116 a         1699 c         1732 g         1025 t
ORIGIN      
        1 ataagctttc aattttggaa gatgtgtccg ctggtactga ctccccttga aaaggggagg
       61 tcgcttggct ccgccaggag caaagcggga ggggatcatt tttcagggga gggagcgcat
      121 caggcgctag ctgccggagt cgtcgacttt ccaatccttc tggaagacgt gaccggcggt
      181 atccctcgct tcagcgcgga aaagttttgc accgttcgag acataggtga agcggatatt
      241 gggatcttcg gagatcgaga tgccgctctc caccgagagc accgggctgt tgtcctgcca
      301 caggcgcagc tcattgatga agaaggcagg aatatacaat agcgtgagct gatccctttg
      361 cagcccggaa ttgttgggat gcccgatcat gatctgcgcc tcgcgcgtgc cgttggtcgg
      421 gctctgatca tccttggcaa actcccgata tttcatctgg ccaagcctgt ttttggcctc
      481 ttcggcgttc ttggccgcag gcgctgagca tccgcctgag gctttcacat aggtcttgat
      541 catatagagc ttgccgtcgc tgagctcggc cacggcatga acgttggtgt aggaattgac
      601 gcgaacgcgc gtcgagattt ccgaaaccct cgcgtcaggt ccgagctcga attttgccgc
      661 catcggagcg ggattttggt cgatcaccag cgtgatcgcc agaacgcgcc tgacatcgtc
      721 ggacgaaagc ttcgtccgca acgttacggg aacgatcgcc gcgtcctcgg cgcggtaggg
      781 catctcgatg gcaatcacgt cgctgccgtc gttcatcggg cgattgttga agatgtcctg
      841 cacgagcccc ggccagggat catagggctc ggccgccgcc acagatgccg gcggcgctcc
      901 aagcgctgca cccagcagac ctgcagcgca aagcaggcga aaacgatgtc cgatcatggt
      961 tgcgttcctc gcggggaccg aagcagcagc ttcactatag ggtattacac aggctattcc
     1021 cactcaatct gcgaatatgc tgcagtggcg ttgcgggcat tgtagtcgtc aaacaatcgc
     1081 cagtttttcc gttcggacgc agccgcagtc tcggcggcgg ccgagatcgg cgtcccacgc
     1141 ttgaccgaga tgcgtacatc tgtcgccaaa gtgtcgagat aacgtcgttc gtcagcaagc
     1201 gccgccggcc aatcgctcac ggatccgtgt ccgggaatca cgcgctctgc gggcagggcg
     1261 ctaagattgc ccatgatctg catccagcct ccaaggctgc cgtccaccac ggggacatgg
     1321 gtgagaaaca gaagatcgcc cgcgaacagc gttttgctgg tctcgtcgaa gatcgtgaga
     1381 tcgctgtcgc tgtgagcagt cggccaggcc cgcagcgtca gggtgcggga gccgaggtcg
     1441 agcttgaggg tgtcgtcgac aagcagcgta ggcggcacga ggcgaacctc gtcgatcagt
     1501 tcatcgccca tgatcccccg gaacgcgtca agatagaatt gtccgcgcat gctgagcgcg
     1561 cggggcaggt tcttgtgacc gacgaagatc gcgccttccg ccaaaaaagc ggcattgcca
     1621 aaggcatgat cgggatgtcc gtgcgtgttg acgacatagc ggatcggttt gtcggtcttc
     1681 gcgcggatcg cggcaagcaa ttgccgtcct tcgcggacgc tgccgccggt gtcgatcacg
     1741 gcgaccgcgt tgtcgcccac gacaaatccc acgttggcga tcgcgccttc gttttcacgc
     1801 gtcatcagtg cgacgtcgcc gacatgcacg aaaacgcccg gcgcgatttc ggtgaccggc
     1861 agcgcttgct gctccgcacg cgcagatatc gatgcagcgg ccaaagcgag cacaatgcaa
     1921 agcaggacgc gatgaaatcc cattcgcctc gaagaatggt tgaagccggt gggttccatc
     1981 acacagatcc gttgcgtcgc tgcgcgcgaa gcggcgctgc cagcgaagat tatcctgttg
     2041 cacgccttaa ttgggaagca tagcattttg gtctcttctt tcgcgtcgcg cgccttatga
     2101 aggggcggca aggccggcag gcctgaaacg aacgaaggcg ggccggaacc gaaaagaatc
     2161 taatgaaaac tgccgtaatc gagcgctgtc tgttgatttc gcttttggcc atccctgcaa
     2221 gcttcgtcag aggcgatgtc gcgcgtgcgc aagcgaacaa tgaaggtctc gatctgtcga
     2281 tcgagctggt cgatcccaag gtgctaaggg tctgcgccga cccgcgcaat ctgccgttct
     2341 cgaacgagaa gggcgaaggt ttcgagaaca agctcgccga atttttcgcc gacaagctgc
     2401 agaagaagct tgattacgtg tactacccgc aggcgaccgg cttcgtgcgg atgacactcg
     2461 gcgctcatcg ctgcgacgtc atcatgggct ttccgcaggg cgacgaactc gttcaaggca
     2521 ccaatcccta ttaccgcacg gcctatgcgc tgatcgccaa gccaggctcg ggccttgaag
     2581 aggttgcaac gctggaagat caacgcctga aaggcaagcg tatcggcatc gtcgccggca
     2641 cgccgcctgc gacctacatg gcggcgaacg gcctgatgtc gaatgcaaag ccctacccgc
     2701 tgatgatcga cacgcgcgtg gattcttccg ccgaagccat gatcaatgat ctcaacaagg
     2761 gcgagatcga ggccggcgtt ctctggggac cgatggcggg tttctacgcc aggaagtcca
     2821 atccgccgct gcatgtcacg cctttggtga aggaaacgtc gggacctcgc ctcgtttatc
     2881 gcatcggcat gggtgtccgt tctgccgatc agaactggaa gcggttgctc aaccgcctca
     2941 tccaggagaa ccagccggcg atcaacaaga tcctgctcga cttcggcgtt cccctgctcg
     3001 acgagaacga tcagccgatc aatgcggaga ccgcgacgaa gtcgccatga tccgacgcgc
     3061 ggccgctctg ttcgttctga catttgcatt tgcgacagcg gcgcccgcgc aagacatcgt
     3121 tcctgaaccc gccgattacc gcaccgaaga ttaccgtgca ccggtgccgg caacgcttgc
     3181 cggcgcccgc gttctcacga cgcgggaggc ggaggcgatc tggcgcgcaa ggagcggcat
     3241 ctttatcgac gtgctgccgc gcgcgccgaa gccgccgaac cttccggccg gcacggtgtg
     3301 gcgcgacaag ccgcggctca acattcccgg cagcgtgtgg ctgcccgata cgggttacgg
     3361 gagactggcg ccgccgaccg agtactatct gcggcaagga ctcgcacgtg cgtccgggcg
     3421 caatcaatca gccctgatcg tggtctattg tcaggctgat tgctggatgt cgtggaacgc
     3481 cgcaaagcga atcctctcat acggctattc aaacgtggcc tggtacccag acggcaccga
     3541 cggctgggaa cgcgccgatt tgccgacaac cgaggcgcag cccgagccgc ggccggcgga
     3601 tgcggagctt cctccgggtt agagcttttg atgcttcatc cccagccgct ttgcttgtgg
     3661 cgccgcagag cgacctccct tttcaaagga ggtcagaatc agatctgtcc ttgcgcgaac
     3721 tactccatct cctgctggat ggtccggccg agcgcgaaca atcgctggtc gatcgtggtc
     3781 ggcacctcgc aaacgaattt catcacgcgc cggcggtcct cgaaaatccg cgtttcccac
     3841 acgagttggt tgccgagctc gtcgaccttg gccgggtcgc gcgatgttgc gtcctgcagc
     3901 gcctgcagcg ccagcgtgtc ggacctgatc ttgtccgcag cgtcgcgctg cttgcgcatg
     3961 acccgctcga gcccgttcat gaccgacgag cgctgctcgt tgagcgtgtc gaacaggcca
     4021 gcgaacagaa gcttgccggc ggtaaccttg tcggcggcgc cggccagata gtccttgatg
     4081 gtcttctcgg cttcatcgag cggcgtccgt cttgccgcaa gcttcgccac cagcgcgctg
     4141 atcttggcgt cgtccttcca tttgtccttg atgtcatcga gcgatgggcc cgcccacacg
     4201 gcgggcaggg agatttcggg gaccttcgcc tgggcacacg gccaatccgg atagcgcgga
     4261 tcggcggcaa agccgggccg cgtcgatatt gcgatcgcaa gcaatgcggc gaaaatgatg
     4321 ggccacctca tgtttcacct ccggcaggcc cgcggcggga gaagccgcgc gccggatcgt
     4381 aggcgaggat cgcgccgatc atgaagatcg tcgtgcaacc ggcgacgacg gcgagcgaaa
     4441 tccagttgat cttgccgtag agcgcaaagc ggatcagctc gaccgcatgg gtaaagggat
     4501 tggcctcgca aacgtaataa agccaagggc ttccctcctg aactcgccat aatgggtaaa
     4561 gcgcggacga ggcaaagaac atagggaaga tcacgaagtt catgacgccc gcaaaatttt
     4621 cgagctgctt gatgccggag gaaatcagca tgccgagcgc gccgagcatc aggccggaca
     4681 ggatcagggc tggcagcacc gtgagatagc cgatcggcgg cggcgtgatg tcccagaacc
     4741 acgcaatcag caggaacgcg tagacctgca gcagcgagac cgccgttccc gccacgagct
     4801 tgcaaaacaa caggtaccag cgcggcagcg ggctcaccag caaggtgcgc atattgccca
     4861 tctcgcgatc atagaccatc gacagcgagg actgcatgcc gttgaagagc tggatcatcg
     4921 ccatcaatcc cggcgcgatg tagacctcat agaggatata ggtctcatac ggcgggatga
     4981 tggagatgcc gagcacctgc cgaaagcccg ccgcgaagat gaagagccac accagggggc
     5041 gcaccagcgc ggagacgaag cgttcgcgct gatgcaggaa gcgcaacgcc tcgcgccaca
     5101 cgatgcccga caggcacgtg gcatattcgg tgagcgcaaa gccgcgccgg cgctggtcga
     5161 caatcgtgga gctcatggcg ggaggctccc ggagagcgca gcggtgccgg tcaggcgcat
     5221 gaaagccgtg ttgacatcgg cagcgccggc ctccttgagg acagttgcgc catcgccgcg
     5281 cgccacgacc cggccctgat gcaggatgac gagatcgtcg ctcggaacga tctcgtcaaa
     5341 cagatgcgtg gcccacagca cgccgatgcc ctggtcgccc acgagctgcc gcacgtgcgc
     5401 aagaatgtcg gcgcgcgcct tcacgtcgag cccgacggtc gcctcgtcga gcagcagaag
     5461 tcgcggccga tgcagcaacg cgcgtgcgat ttcgagccgc cgcatctggc cgcccgagag
     5521 gtcgcgcacc ttgctgccgg cgcgatcggc aaggccgagc cgtgcaagca cc
//
LOCUS       DATLJT010000024         8090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1379494,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000024 DATLJT010000000
VERSION     DATLJT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379494"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VM910_00515"
     CDS             complement(<1..426)
                     /locus_tag="VM910_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61067.1"
                     /translation="MTGFAIRLVTLTLFFGALVATPAVMPAFAAGGGGGGGGGPPGED
                     IMRPSAPPANTKSPATTRSTHRAKKSDKQSFLNDPAFIQGYRDAYDTIYDRYDYETAI
                     EKLRALGHDDYANVANLIGYSYRKLGDYRQSQIWYERALK"
     gene            663..935
                     /locus_tag="VM910_00520"
     CDS             663..935
                     /locus_tag="VM910_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HVI61068.1"
                     /translation="MTEQTDTRPLPLGLARRGYRGVIQRLSAAKAGSALPDVELKSRL
                     IELGFVEGARVEVLHEGIVGHDPIAVRVENVTIAVRRREAMAVIVA"
     gene            954..2825
                     /locus_tag="VM910_00525"
     CDS             954..2825
                     /locus_tag="VM910_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HVI61069.1"
                     /translation="MEAPLMHLALVGTPNSGKTSLFNALTGSRQKVANYPGVTVERKE
                     GSFVTPKGRQVSVVDLPGTYSLRGRSPDEEITRDIVLGRTSGEHVPDLVLCVADSTNL
                     RLTIRLLLELKSTGRPLALVLNMYDIATRRGVNVDVPRLSEALGVPVVTSIAVRKGGT
                     ADLLRLTDDILAQAPTKPRENLWEPLTVSQLRATQREADRIIAATVSLPTRPDTWTAR
                     IDAVVLHPVAGLAILLLILFVMFQAVFAWAQPLMQLLSSAFDAAGQFVHDTLPGGLLQ
                     SFLQNGVISGVGSVVVFLPQIIIIFLFILLLEDFGYMARAAFLMDRIMGGAGLHGRAF
                     IPLLSSFACAIPGIMATRVIDNRRDRLTTILIAPLMTCSARIPVYTLIISAFIPARDV
                     FGWINLQGLVMFGLYGAGIASALGVSFLIKFFMWRDYQPAPFMLELPDYKMPRARSVA
                     IGVYNRAKMFLQRAGTTIFAMMVLIWFLASFPQPPAGADGPAINYSLAAMIGKVIAPL
                     LAPVGFNWQIAVALIPGMAAREVAVAALGTVYAIEGGKEAAAQIGQVLASKWSLATAL
                     SLLAWYIFAPQCASTLAVIRRETGSWKWMAATFAYMLALAYAASLATYNVAVALGAG"
     gene            complement(2828..3034)
                     /locus_tag="VM910_00530"
     CDS             complement(2828..3034)
                     /locus_tag="VM910_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61070.1"
                     /translation="MDSINIRSAAGEAQSDRSPVDTAWLVLEAANDLGDEATVEVCRR
                     VIDASLRGMPPAQADLHLVVDYFR"
     gene            complement(3069..4193)
                     /locus_tag="VM910_00535"
     CDS             complement(3069..4193)
                     /locus_tag="VM910_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HVI61071.1"
                     /translation="MNQAKSYLEDLDDAISRGTAESRSRALWHATDLLIAGRYSEEEI
                     ATFGKVISRLAEEIEVEVRAQLSNRIACFDRAPIDVIHKLAFDDSIKVAGPVLEQSER
                     LDDDALIANARTKSQAHLLAISKRKSIGETVTDVLVARGNRAVVNSVAGNAGARISGS
                     SLLHMVKRAEGDSILAEQLGLRKDVPRHLFQQLIAKATDDVRKRLEHERPEMVDEIGT
                     SVIDVAGTLQSKFGPVSRSYFVAKRVVTTQYRLGNLNEASISGYAMTHKFEEVTIGLS
                     LLSALPVDVIERAMVDRNREMLLILAKALKFSWQTTMALLFLGAKDHRITARELTALE
                     RDFNRLNVETSERVLEFYRSRRNPGSAEPGLGAEPLVAAG"
     gene            complement(4391..8080)
                     /locus_tag="VM910_00540"
     CDS             complement(4391..8080)
                     /locus_tag="VM910_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVI61072.1"
                     /translation="MQTTLLGLAIAFIVALLAALIGPYFIDWNQFRPQFEAEAAKVIG
                     APVRVAGGLDARLLPTPTLRLRSVVVGGANDLGKVRADKLDVEFSLGELMRGEWRATE
                     LSIDGMALDLGLDRDGRIDWPASSGAFDLGSLSIDRLNLTGRVALHDAATRKTLELND
                     IAYSGDVRSLAGSLRGRGSFVLAGTRYPFRVSSGQGQGGSGTRVHLMVDPTERTPALD
                     LDGILGFEARAPRFEGAAILAVPPRKAKGNQPMPTPWRITARVKADHAAAQLEQIEAS
                     YGADDRALKFAGAGDIRFGASPLLRATLSARQLDADRLFASDGGKDNSAAEPVRLLPA
                     LRAALAALPPVPVPAKIDASAEQIMLGGRPLQNLTLSLRSDGGNWAVDRLDARAPGTT
                     QVSFESVATGSGGGASSANLSGRLDVDSSDSDVLMAWLQGRSDIGFHSQKPFRLHGVV
                     GVTDDRITVDALKAEIDGGTVEGRLALSGAVAGGGANFEAALKAQRLDLDAAAAFVRS
                     IAPVQADWPQQATVSLDIDRAISAGQELRSFGAKFGYSPRTLSLELLKFGQANGVAIE
                     GTGSFDRGDATGKLTLSANAVSIARITAMLAPLAPALAARLNPLAVQPGPARLKLALN
                     IDKNAQEHDRANARAVLDVDAPQLRGVVTVTAKPPLAAIRDVDLDQLSRGEASVETKL
                     SADRGDALLTLLGLDHVLAAGEGTVQFEGSASGVWRAPVRLSAKMWGSGIDAEAQGTA
                     EPWAQAPKANVNLRVRSVNLAPLFGLKPSDSVAQNVRLFGHLALAGNKLTFDDLDSVV
                     ASSRLRGRLAVTLEQTREVDGEVGVDALDLAPAFALAIGAAGREASAPLGTGLLRGWR
                     GRVAFQALSAVLPGGAELRPLGGVVKSDGQSLAFDALKGKIGGGDVSANVDARQDANG
                     IALNARLELSGIDGNALHYRSLKMPAGRASLQMTLISQGRSVQALTGAVSGSGTVTLE
                     RASIAGLDPRAFEVAIRASDSGQATDDARLRQIVDPVLAAGTLPVTSAQIPFNIRDGR
                     LRVDAATLDAANARAIISGGYDFAADQTDIRASLTSTATGTASSRPEIQLFVVGPPDA
                     LNRTVDVGSLSSWLAVRTIDRETRRLDAIERGEPPPADPASIPPATAALPSTTVPDAG
                     PLEVPLPGRDPRRPQPRAKAAPPRPPQLGGPSSASQPSAPQPIASQVAPLPPPVEVRP
                     PPGPPAQAKPKPRPPLPLRPPANNP"
BASE COUNT         1370 a         2672 c         2574 g         1474 t
ORIGIN      
        1 tttgagcgcg cgctcgtacc agatctggga ctgcctgtag tcgccgagct tgcggtagga
       61 atagccgatc aggttggcga cattggcgta gtcgtcgtgt ccgagcgccc tgagcttctc
      121 gattgcggtt tcatagtcgt aacgatcgta gatcgtgtca taggcgtcac gatagccctg
      181 gatgaaagcg gggtcgttga ggaatgattg cttgtcggat ttcttggccc gatgggtcga
      241 acgggtggtc gcgggagatt tcgtgttcgc cggcggcgcc gacggcctca tgatatcctc
      301 gcccggaggt cctcctccgc ctccaccacc gccgccggcc gcaaaggccg gcatcacagc
      361 cggtgtcgcg acgagcgctc cgaagaaaag tgtgagtgtc accaacctga tcgcaaatcc
      421 ggtcatgtat ccctcctgtt tgcaggttgt ccgcggttcc tccagccgct gctgtaaaac
      481 cccatttgcc ttgcgacatt ccccgcctcg atcgggctcg ccgaaaacat gaatgtgatc
      541 ggccaccggc gtgtcggcat ttgcgccagg tcaaacgccc ggcttcttaa aatcgctcta
      601 aggtaaccct cgcgggaacg tgcgcgcttg acctgccgcg tcacgattga aaatagagat
      661 tgatgaccga acagactgat actcgccctc tgccgctcgg ccttgcccgg cgcggttatc
      721 gcggcgtgat ccagcgcctt tctgccgcca aagcggggtc ggcattgccg gacgtcgagc
      781 tgaaaagccg cctgatcgaa ctcggctttg tcgagggcgc tcgggtcgag gtgctgcacg
      841 agggcatcgt cgggcatgat ccgatcgcgg ttcgcgtcga aaacgtcacc atcgcggtgc
      901 gccggcgcga ggcgatggcc gtcatcgtcg cctgacgggc cggacacaac gcgatggaag
      961 ctcctctgat gcatcttgcc ctggtgggca cgccgaacag cggcaagact tcgctgttca
     1021 atgcgctgac gggcagccgg cagaaggttg cgaactatcc gggcgtcacc gtcgaacgca
     1081 aggaaggatc ctttgtcacg ccgaagggcc ggcaggtctc cgtagtcgat ctgcccggca
     1141 cctattcgct gcgcggacgc agcccggatg aggagatcac gcgcgacatc gtgctcggcc
     1201 gcaccagcgg tgagcacgtg cctgacctgg tgctgtgtgt cgccgactcg accaatctgc
     1261 ggctgaccat ccgtctcctg ctcgaactca agagcaccgg ccgtccgctc gcgctggtac
     1321 tcaacatgta cgatatcgcc acacgccggg gcgtaaacgt cgacgtgccg cgactgtcgg
     1381 aagcgctcgg cgtaccggtc gtcacttcga tcgccgtgcg caagggcggc accgccgatc
     1441 tcctgcgcct gaccgacgac atactggcgc aagccccgac caagccccgc gaaaacctct
     1501 gggagccgct cacggtatcc caattgcggg cgacgcaacg cgaggccgac cgcatcatcg
     1561 cggcgaccgt gagcctgccg acacggcccg acacctggac cgcgcggatc gatgccgtgg
     1621 tgctgcatcc cgtggctggg ctggcgatcc tgctgttgat cctgttcgtg atgttccagg
     1681 cggtgttcgc ctgggcgcag ccgctgatgc aactgttgtc ttcggctttc gatgccgccg
     1741 ggcagttcgt ccatgacacc ctgccgggag gcctgttgca aagcttcctg cagaacggag
     1801 tgatctccgg cgtcggcagc gtcgtggtgt tcctgccgca gatcatcatc attttcctgt
     1861 tcatcctgct gctcgaagac ttcggctata tggcgcgcgc agcgttcctg atggaccgca
     1921 tcatgggcgg cgccggattg catggccgcg ccttcattcc gctgctgtcg agctttgcct
     1981 gcgcgatccc gggcatcatg gcgacaaggg tcatcgacaa ccgccgcgac cggctgacca
     2041 ccatcctgat cgcgccgctg atgacgtgct cggccagaat tcccgtctac acgctgatca
     2101 tctccgcctt cattccagcc agggacgttt tcggctggat caatttgcag ggtctcgtga
     2161 tgttcggcct ttatggcgcc ggcatcgcga gcgcgctcgg cgtgtccttc ctgatcaagt
     2221 tcttcatgtg gcgcgattat cagcccgcgc ctttcatgct ggaattgccc gactacaaga
     2281 tgccccgcgc gcgcagcgtc gcgatcggcg tctataaccg cgcaaagatg ttcctgcagc
     2341 gcgccggcac cacgatcttc gcgatgatgg tgctgatctg gtttctggcc tcgtttccgc
     2401 aaccgccagc cggagccgat gggcccgcga tcaactacag cctggccgcc atgatcggga
     2461 aggtcattgc gccattgctt gcgccggtcg gcttcaactg gcagatcgcg gtcgccctga
     2521 tcccggggat ggccgcgcgc gaagtcgcgg tcgcagccct cggcacggtc tatgccatcg
     2581 aaggtggcaa ggaggcggcc gcacagatcg gccaggtgct cgcctccaaa tggagccttg
     2641 cgaccgcgct gtcgctgctc gcctggtaca ttttcgcccc gcaatgcgca tcgacgctcg
     2701 ccgtgatccg ccgcgaaacc ggaagctgga aatggatggc ggcgaccttc gcctatatgc
     2761 tggcgctcgc ctatgcggcg agccttgcga cctacaatgt cgctgtggcg ttaggcgcgg
     2821 gctgaaatca cctgaaataa tcgaccacga ggtgaaggtc ggcttgcgcg ggcggcatgc
     2881 cgcgcaggct cgcgtcaatc acgcggcggc agacttcgac ggtcgcttcg tcgccgagat
     2941 cgttggcggc ttcaagcacg agccaggccg tatcgaccgg cgacctgtcc gactgggctt
     3001 cgcccgctgc agagcggatg ttgatcgagt ccatggtcaa tttcctcagg tcaacgcagt
     3061 atcgctgctc agcccgccgc gaccagaggc tcggcgccaa ggccgggctc cgccgatccg
     3121 ggattcctgc gcgaacggta aaattcgaga acgcgctccg acgtctcgac gttgagacga
     3181 ttgaaatcgc gctccaacgc ggtcagctcg cgtgccgtga tgcggtgatc ctttgcgccg
     3241 aggaaaagca gcgccatggt ggtctgccag gaaaacttca gcgctttggc caggatcagc
     3301 agcatctccc ggttcctgtc gaccatggcg cgctcgatca cgtcgaccgg aagcgcggac
     3361 agcagcgaca gtccaatcgt gacctcttcg aatttatgcg tcatcgcgta gccggatatc
     3421 gacgcctcgt tcaggttgcc caaccggtac tgggtcgtca cgacgcgctt ggccacaaaa
     3481 tagctccgcg acaccggacc gaatttggac tgcagtgtgc ccgcgacgtc gatcaccgag
     3541 gtgccgattt catcgaccat ctcggggcgc tcgtgttcga gccgtttcct gacatcgtcg
     3601 gtggccttgg cgatgagctg ctggaacagg tgacggggga cgtccttgcg cagcccgagc
     3661 tgctcggcga gaatcgaatc gccttccgcc cgctttacca tgtgcaggag gctggaaccc
     3721 gaaatgcgcg caccggcgtt gccggcgacc gagttgacga cggcgcgatt gccgcgcgcg
     3781 acaagcacgt cggtcaccgt ctcgccgatc gacttgcgtt ttgagatcgc aagcaggtgc
     3841 gcctgactct tggtgcgcgc gttggcgatc agggcgtcgt catcgagtcg ctccgattgc
     3901 tcgagcactg gacctgcgac cttgatcgaa tcgtcaaagg cgagcttgtg gatgacatcg
     3961 atcggcgccc ggtcaaaaca ggcgatgcga ttggagagct gcgcccgcac ttcgacctcg
     4021 atctcttccg caaggcggct gatcaccttg ccgaaggtgg cgatctcctc ttcgctgtag
     4081 cggcccgcga tcagaagatc ggtcgcgtgc cagagcgcgc gcgagcggct ttccgccgtc
     4141 ccccgcgaaa ttgcatcgtc aaggtcctcc agatacgatt tcgcctggtt catcctgcaa
     4201 gccttcaaaa acacaaacga gcgcgcgcat gcgcatcgac agcgtcgcat gctagaggtc
     4261 gagtgcgagt attccgtaaa atcggcaaat aagtattgtt gttcaaaatt cgctaaaatg
     4321 cgcgcgattt cgttcatcct taatcacgca cgacctgcgg atgccggtgc ttgcccctga
     4381 cggcataacg tcaggggttg ttggctggcg gcctcagcgg cagcggcggc cgcggtttcg
     4441 gctttgcttg cgccggcggt cccggcggcg gccgcacctc gaccggtggc gggagcggcg
     4501 ccacctggct tgcgatcggc tgcggcgccg aaggttgcga tgcggacgac ggcccaccga
     4561 gctgcggcgg ccgcggcgga gcggccttcg cccttggctg cggacgccgc ggatcacggc
     4621 cgggcagcgg cacctcgagt ggtcccgcat cgggtaccgt ggttgacggc aacgcggcgg
     4681 tcgccggcgg aatcgacgcc ggatccgcgg gcggcggctc gccgcgctcg atcgcatcga
     4741 gcctgcgcgt ttcgcggtcg attgtccgca ccgcaagcca cgacgacaac gagccgacat
     4801 ccaccgtgcg attgagcgca tccggcgggc ccactacaaa aagctggatt tccgggcggc
     4861 tgctcgccgt accggttgca gtggacgtca ggcttgcgcg aatgtcggtc tggtccgcgg
     4921 cgaaatcgta gccgcccgag atgatggcgc gcgcgttcgc cgcatcgagc gtcgccgcat
     4981 cgacgcgaag gcgcccatcc ctgatgttga aggggatttg ggccgacgtt accggcaacg
     5041 tgcccgccgc cagcactgga tcgacgatct gccgcagcct tgcatcatcg gtcgcctgac
     5101 cggagtcgct ggcacggatc gcaacctcga acgcgcgcgg gtcgaggccg gcgatgcttg
     5161 cccgttccaa cgtcactgtt ccgcttccgg aaaccgcgcc tgtcaacgcc tgaacgctgc
     5221 ggccctggct gatcagcgtc atctgcagcg aggcgcggcc tgccggcatt ttgagagagc
     5281 gatagtgcaa agcgttgccg tcgatgccgg acagttcgag gcgtgcattc agcgcaatac
     5341 catttgcgtc ctgtctcgcg tcgacattcg cgctgacgtc gccgccgccg atcttgcctt
     5401 tgagcgcatc gaatgccagc gattgaccgt cgctcttgac gacgccgccg agcggccgca
     5461 actcggcgcc acccggcagc acggcgctca gcgcctgaaa tgcgacccgg ccgcgccaac
     5521 ccctcaggag tcccgtgccg agcggcgcgg acgcttcacg gcccgcggcg ccgatcgcga
     5581 gcgcgaatgc gggcgcgagg tcgagtgcgt cgacaccgac ttcgccgtcg acctctcttg
     5641 tctgctccag ggtcaccgcg agacgtccgc gcaaccgcga gctggcgacg acgctgtcca
     5701 gatcgtcgaa cgtcaatttg ttgccggcga gcgcgagatg cccgaacagc ctgacgtttt
     5761 gcgccacgct gtcggatggc ttgaggccga acagcggcgc gaggttgacg ctgcggaccc
     5821 gcaaattgac gttggctttc ggggcttgcg cccagggctc cgccgttccc tgggcttcgg
     5881 cgtcgatgcc actcccccac atttttgcac tcaaccgcac aggcgcgcgc cacacgccgc
     5941 tcgccgatcc ctcgaattgc accgttcctt cgccggctgc gaggacatga tcgagcccaa
     6001 gcagggtgag cagcgcgtcg ccccgatctg ccgacaattt cgtttcgacg ctggcctcgc
     6061 cgcggctgag ttggtcgaga tcgacgtcgc gaatcgcggc aagcggaggc tttgccgtaa
     6121 ccgtcacaac acctctgagc tgcggagcgt cgacgtcgag aaccgcgcgc gcattggcgc
     6181 gatcatgttc ttgcgcgttc ttgtcgatgt tgagcgccaa tttcaggcgg gccggcccgg
     6241 gctggactgc aagcggattc aaacgcgcgg caagagccgg tgccagcggc gcgagcatcg
     6301 cggtgatccg ggcgattgaa acggcatttg cgctcagggt gagtttgccg gtggcatcac
     6361 cgcgatcaaa acttcctgtg ccctctatcg ccacgccatt cgcctggccg aacttcagga
     6421 gttccagcga gagcgtcctg gggctgtagc cgaattttgc gccgaacgaa cgcagctcct
     6481 ggcccgccga tatcgcgcgg tcgatatcca gcgagacggt tgcctgctgc ggccaatcgg
     6541 cttgcacggg cgcgatcgat cgaacaaagg ccgcagccgc gtcgagatcg agccgctggg
     6601 ctttcagcgc cgcctcgaaa tttgcacctc cgcccgccac ggcgcccgac agcgcaagac
     6661 ggccctcgac ggtcccgccg tcgatctccg ccttcagtgc gtcaaccgtg atgcgatcgt
     6721 cggtcacgcc gaccacaccg tgcaggcgga acggcttctg gctgtggaag ccgatatcgc
     6781 tgcggccttg cagccacgcc atcagcacat ccgaatcgga ggagtcgacg tccagcctgc
     6841 cggacagatt ggcggacgat gctccgccgc cggagccggt cgcaacgctc tcgaaggaga
     6901 cctgggtcgt gccgggcgcc cgcgcgtcca ggcgatcgac cgcccagttc ccgccatcgc
     6961 tgcgcaagct cagggtgagg ttttgcaacg ggcgaccgcc cagcatgatc tgctcggcac
     7021 tcgcgtcgat tttcgcgggg accggcacgg gcggcagcgc ggccagcgcc gcgcgcagtg
     7081 caggcaacag ccgcaccggc tcggcagcgc tattgtcctt gccgccgtcg gacgcaaaaa
     7141 gtcggtccgc atcgagctgg cgcgccgaca gggtcgcgcg caggagcggc gaggcgccaa
     7201 agcggatatc gcctgcaccg gcaaatttca gcgcgcgatc atccgcgcca tagcttgcct
     7261 cgatctgttc gagctgggcc gcggcatggt cggccttgac cctggccgtg attcgccaag
     7321 gcgtcggcat aggctggttg ccttttgcct tgcgcggcgg cacggccaaa atggctgcac
     7381 cctcgaagcg cggcgcacgc gcctcaaagc ccaggatccc gtcgagatcg agggccggcg
     7441 tgcgctcggt cggatcgacc atcagatgca cgcgggtacc gctgccgccc tggccctgcc
     7501 ctgacgagac gcgaaacgga taacgcgttc ccgccaagac aaagcttccg cggccgcgca
     7561 acgatccggc cagcgaacgc acgtcgcctg aataggcgat atcgttcagt tccagcgtct
     7621 tgcgggtcgc ggcatcgtgc agggcaacgc ggccggtgag attcaaccgg tcgatcgaga
     7681 gcgagccgag atcgaacgcg ccgcttgaag ccggccagtc gattcgcccg tcgcggtcga
     7741 ggccgagatc gagagccatg ccgtcgatgc tcaactcggt ggcccgccat tcgccgcgca
     7801 tcaattcgcc gagcgagaac tcgacgtcga gcttgtcggc gcggaccttg ccgagatcat
     7861 tggcaccgcc gaccaccacc gagcgcaatc gcaaggtggg cgtcggcaac agccgcgcgt
     7921 cgagcccgcc cgcaacccgc accggcgcac cgatcacctt ggcggcttcg gcctcgaact
     7981 gcggccgaaa ctggttccag tcgatgaaat aaggcccgat cagcgcggcg agcaacgcaa
     8041 cgatgaaagc aattgccagt ccgagcagcg tcgtctgcac ggggtctccc
//
LOCUS       DATLJT010000025         4249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2757970,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000025 DATLJT010000000
VERSION     DATLJT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757970"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..962)
                     /locus_tag="VM910_00545"
     CDS             complement(3..962)
                     /locus_tag="VM910_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HVI61073.1"
                     /translation="MTRLRCAILDDYYNLALTIADWSKITDRVDITVFNQPFASQEAA
                     ATALKDFEIICAMRERTAFPRSLFAALPKLKLLITSGMRNLAIDMEAAKDHKVVLCGT
                     QWARDPTAPLTMGLILELTRKIGRENSRMHGGESWQKFVGIEIEGRTLGVVGLGKLGG
                     KVSKLAQAFGMNVIAWSPNLTADRCKEVGVTYATKEELFSTADIVTVHVVLSPRSRGL
                     VGREDLARMKPTAYLVNTARGPIVDEQALLETLQQRKIAGAGIDVFSVEPLPLDHPFR
                     KLDNMVLTPHLGYATEESFRNHYTQMVEGIDAWFKGESPRRLA"
     gene            complement(1094..1312)
                     /locus_tag="VM910_00550"
     CDS             complement(1094..1312)
                     /locus_tag="VM910_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61074.1"
                     /translation="MATIISASLRAPAVDRAGQSSPVTGFKAFWQRFFATAFHPYRPE
                     LHYMRGPGPAWRAKYGDQALYRLKSGDV"
     gene            1629..3281
                     /gene="ettA"
                     /locus_tag="VM910_00555"
     CDS             1629..3281
                     /gene="ettA"
                     /locus_tag="VM910_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HVI61075.1"
                     /translation="MARQFVYFMQGLSKTYPGNRKVLDNVHLSFYPDAKIGVLGVNGS
                     GKSTLLRIMAGIDKEYNGEAWVAEGARVGYLEQEPQLDAKLTVRENVMQGVAKQKAIL
                     DRYNELATNYSDETADEMTRLQDEIEAAGLWDLDSKVDQAMDALRCPPDDADVTKLSG
                     GERRRVALCRLLLDQPELLLLDEPTNHLDAESVSWLEGHLRQYPGAILIVTHDRYFLD
                     NVTGWILELDRGRGIPYEGNYSSWLVQKQKRLAQEGREEAARERTLAREQEWIAASPK
                     ARQAKSKARYTRYEELLKQASEKQTQTAQIIIPVAERLGANVIDFEGLSKGYGDRELI
                     EDLTFKLPPGGIVGVIGPNGAGKTTLFRMITGQEKPDKGTITIGESVHLGYVDQSRDA
                     LDGSKTVWEEVSGGNELILLGKREVNSRGYCSSFNFKGADQQKKVGSLSGGERNRVHL
                     AKMLKSGANVLLLDEPTNDLDVDTLRALEEALEDFAGCAVIISHDRWFLDRIATHMLA
                     FEGDSHVEWFEGNFQDYEKDKMRRLGQDSIIPHRLKYKKLTR"
     gene            3281..>4249
                     /locus_tag="VM910_00560"
     CDS             3281..>4249
                     /locus_tag="VM910_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI61076.1"
                     /translation="MMRRLALIAAVVAAVCGSLPACAADAAFTQFVTSLWPEAQAAGV
                     SRATFDRETAGLEPDYKLPDLILPGRPPTGAPAQAEFVQVPADYIREASIARLAGEGQ
                     KLMQQYRDALAAIEARFGVPGTIVLAIWGRETDYGRYTLPYDAVRVMATQAYVGRRKD
                     TYRNEFILALKILGEGAVSRKDMRASWAGATGLTQFLPSEYYKHGVDLDGDGRIDIWH
                     SVPDALASAAKQLVDKGWQKGVRWAYEVTAPANVDCTMGVPDVTRPISEWLRAGFVPV
                     RGQGLTPAEQAEPASLLQPEGIYGPSFLTTKNYFVIKEYNFSDLYVL"
BASE COUNT          806 a         1332 c         1362 g          749 t
ORIGIN      
        1 agctacgcca atcgccgggg cgactcgccc ttgaaccagg cgtcgatgcc ttcgaccatc
       61 tgggtatagt gattgcgaaa gctttcctcg gtggcgtagc cgagatgcgg cgtcagcacc
      121 atgttgtcga gcttgcgaaa gggatgatca agcggcagcg gctcgaccga gaacacgtcg
      181 atgccggcac ctgcgatctt cctctgctgc aaggtctcga gcagcgcctg ctcgtcgacg
      241 atcggcccgc gcgcggtgtt gaccagatag gctgtcggct tcatgcgcgc gaggtcttcg
      301 cgcccgacca gcccccgcga gcgtgggctc agcaccacat ggacggtgac gatatcggcg
      361 gtcgaaaaca gttcctcctt ggtggcatag gtcacgccga cttccttgca gcggtccgcc
      421 gtcaggttcg gactccaggc aatcacgttc atgccgaagg cctgcgccag ctttgagacc
      481 ttgccgccga gcttgccaag gcccaccaca ccgagcgtcc gtccttcgat ctcgatgccg
      541 acgaatttct gccaggactc gccgccatgc atacgcgagt tctcgcggcc aatcttgcgc
      601 gtcagctcca ggatcaggcc catcgtcagc ggcgcggtcg gatcgcgcgc ccattgcgta
      661 ccgcacagca cgaccttgtg gtccttggcg gcttccatgt cgatggcgag gttccgcatc
      721 cccgaggtga tcagaagctt cagcttgggt agcgccgcaa acaggctgcg cggaaacgcc
      781 gtgcgctcgc gcatcgcgca gatgatctcg aaatccttca gcgcggttgc ggctgcttcc
      841 tgggaggcga agggctggtt gaaaacggtg atgtcgacgc ggtcggtgat cttcgaccag
      901 tcggcgatgg tcaaagcgag attgtaatag tcgtcgagga ttgcacagcg cagccgcgtc
      961 attggggagg tccgttcaga gcgaggtgac ggcccggaag gaaggccgac agaacgtccc
     1021 atcgttgcgc gcccggcgca ggcgcgcaag ccgcgtcaag cttcaaaatt gccctggaac
     1081 aggtgccggc ggctcagacg tcgccggatt tcaagcgata gagcgcctga tcgccatact
     1141 tggcgcgcca ggcggggccc gggccgcgca tgtaatgcag ttccggccgg taggggtgaa
     1201 aggcggtggc gaagaagcgc tgccagaagg ccttgaaccc ggtgaccggg ctggattgtc
     1261 cggcgcggtc gaccgccggg gcccgaaggg aagccgaaat gatcgttgcc atgacgcggg
     1321 ctcgctgttc ttgtccgcgg ctctggccgc gtttgacgcg gctttgccgc gtcggaagcc
     1381 agctttggcc cgattcatta aaagatagtt tcaattgttc tgatttgccg gagtcatggt
     1441 atcgagccgg tattcgtccg tggtgaacga attgaaaacg agtgcccgga ccgccaaatc
     1501 gcctctctcg cggcgacccg ggaggaaact tctgaaccgc cgaggctggt tgctctttcg
     1561 cggccccgcc gctacatgag ccgcagcaaa attccgacaa atcgcaggat ttaccgttag
     1621 gcagctcaat ggcccgtcag ttcgtctatt tcatgcaggg tctgtccaag acctatcccg
     1681 gcaatcgcaa ggtgctcgat aacgtccatc tgtcgttcta tccggacgcc aagatcggcg
     1741 tgctcggcgt caacggttcc ggcaaatcga cgctgctcag aataatggcc ggcatcgaca
     1801 aggagtataa cggcgaggct tgggtcgccg agggcgcccg cgtcggctat ctcgagcagg
     1861 agccgcagct cgatgccaag ctcaccgtgc gcgagaacgt catgcagggc gtggccaagc
     1921 agaaggcgat cctcgaccgc tacaacgagc ttgccacgaa ctactcggac gagaccgccg
     1981 acgagatgac caggctgcag gacgagatcg aggcggcagg cctttgggat ctcgacagca
     2041 aggtcgacca ggcgatggac gcgctgcgct gcccgcccga cgacgccgac gtgaccaaac
     2101 tgtcgggcgg cgagcggcgc cgcgtcgcgc tgtgccgcct tttgctcgac cagcccgagc
     2161 ttctgctgct cgacgagccg accaaccatc tcgacgccga atccgtgtcg tggctggaag
     2221 gacatttgcg ccagtatccc ggtgcgatcc tgatcgtcac ccacgaccgc tacttcctcg
     2281 acaatgttac gggctggatc ctcgagctcg atcgcggcag aggcatcccc tacgagggca
     2341 attattcctc ctggctggtg cagaagcaga agcggcttgc gcaggagggc cgcgaggagg
     2401 ccgcccgcga gcgcacgctg gcgcgcgagc aggaatggat cgcggcttcg ccgaaggcgc
     2461 gtcaggcgaa atccaaggcg cgctatacgc gctatgagga actgctcaag caggcgagcg
     2521 agaagcagac ccagaccgcg cagatcatca tccccgtcgc cgagcggctc ggcgccaatg
     2581 tcatcgactt cgagggcctg agcaaaggct atggcgaccg cgagctgatc gaggatctca
     2641 ccttcaagct gccgcccggc ggcatcgtcg gggtgatcgg cccgaacggc gccggcaaga
     2701 ccacgctgtt ccggatgatc acgggccagg aaaagccgga caagggcacc atcaccatcg
     2761 gcgagtccgt ccatctcggc tatgtcgacc agtcgcgcga cgcgctcgac gggtcgaaaa
     2821 ccgtgtggga ggaggtttcc ggcggcaacg aattgatcct gctcggcaag cgcgaggtca
     2881 attcgcgcgg ctattgctcg tcgttcaact tcaagggcgc cgaccagcag aagaaggtcg
     2941 gctcgctgtc gggcggcgag cgcaaccgcg tgcatctcgc caagatgctg aaatccggcg
     3001 ccaacgtgct gctgctcgac gagccgacca acgatctcga cgtcgacacg cttcgcgcgc
     3061 tggaagaagc gctggaggat tttgccggct gcgccgtgat catcagccat gatcgctggt
     3121 tcctcgaccg catcgcaact cacatgctgg ccttcgaagg cgacagccat gtcgaatggt
     3181 tcgaaggcaa tttccaggat tacgagaagg acaagatgcg caggctgggt caggattcca
     3241 tcatcccgca tcggctgaag tacaagaagc tgacgcgatg atgatgagga ggttggcgct
     3301 catcgctgcg gtggtcgcag ccgtctgcgg ctcattgccc gcctgcgccg ccgacgccgc
     3361 cttcacacaa ttcgtcacct cgctctggcc ggaggcgcaa gccgccggcg tctcgcgcgc
     3421 gacgtttgat cgcgagaccg ccggcctcga gcccgactac aagctgcccg atctgattct
     3481 gccggggcga cccccaaccg gtgcgccggc gcaggcggag ttcgtgcagg tgccagccga
     3541 ctacatcagg gaagcctcca tcgcgcgcct tgcgggcgag gggcagaagc tgatgcagca
     3601 atatcgcgat gcgcttgccg caatcgaagc gcgtttcggc gtacccggca ccatcgtact
     3661 cgcaatatgg ggccgcgaga ccgattacgg ccgctacacg ctgccctatg acgcggtgcg
     3721 cgtgatggca acccaggcct atgtcggccg gcgcaaggat acttatcgga acgagttcat
     3781 cctcgcgttg aagatcctgg gcgaaggcgc cgtcagccgc aaggacatgc gcgcctcctg
     3841 ggccggcgcg accgggctca cgcagtttct cccatccgaa tactacaagc acggcgtcga
     3901 tctcgatggc gacggccgca ttgacatctg gcattccgtg ccggatgcgc tcgcctcagc
     3961 cgcaaagcag cttgtcgaca agggttggca gaagggtgtg cgttgggcgt atgaagtgac
     4021 ggcgccggcc aatgtcgact gcaccatggg cgtgcccgac gtgacgcgac cgatcagcga
     4081 atggctgcgc gcgggctttg tgccggtccg cggccaaggg cttacgccgg ccgaacaggc
     4141 tgagccggcg tcgctgctgc agcccgaagg catctacggg ccgtcctttc tgaccacgaa
     4201 gaactacttc gtcatcaagg aatacaattt ctccgatctc tatgtgctg
//
LOCUS       DATLJT010000026         4284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4366276,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000026 DATLJT010000000
VERSION     DATLJT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4366276"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2763)
                     /locus_tag="VM910_00565"
     CDS             complement(<1..2763)
                     /locus_tag="VM910_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI61077.1"
                     /translation="MPSIFAEPTYGMVFRYDGELLSVVAHYNLDQTALDAYQKIWPMQ
                     PDNRTATGRTVLERKIVHIPDVEQEPRYTFAAAYRASIGIRTYLGVPMLRDGNPIGAI
                     ALYRGHVALFSDREIELVKAFADQAVIAIENARLLNELRESLQQQTATSEVLRVISSS
                     PGELEPVFQAMLANAVRICEAKFGTLYLCEGDALRTIAMYGAPPAFAEARRRDPMVRP
                     NPETTLGRALATKRAVQIGDVQDEPGYANAPTGTTGAQFAKLSGARTVLAVPMVKENE
                     LVGAIVIYRQQVRPFTDKQVELVTNFAAQAVIAIENTRLLNELRQSLQQQTATADVLK
                     VISRSTFDLQSVLDTLTASAARLCEADRGAVMQRDGDVYRLASNYGYSREAERYALEH
                     PLRPERGSVTGRAALESRPFHIPDVLADPEYEATGYQQALGYRTILAVPLLREGTTIG
                     TFTLTRVKVNSFTDKQIELVTTFADQAVIAIENARLLSELRQSLQQQTATADVLKVIS
                     RSTFDLQVVLDTLVESAVRLCEADIGHIARPNEAGLFHTQAYFGYSREFKEVLERTPI
                     KAGRESVIGRALLEARAVQVADVLAEPNYALIEVQRIGGFRTVLGMPLMREGTPIGVF
                     GLARNSVRPFSDKQIELVTTFADQAVIAIENVRLFEETKEALERQTATGEILASISGS
                     MTDPKPVFDAIVRNLLRLFGTRFAGVQLLNDGIIEMPAADGAPGFEKILKRFPRPLDD
                     TTAAGRTMLARQVVQYSPLRGNPKAPSATRQFAQEFGYDSMIYAPMVRGDKVIGAIGT
                     AHHEPRVFDDREVALIKSFADQAVIAIENVRLFDEVQQRTRELSESLQQQTATADVLK
                     VISRSTFDLKAVLQTLVESAGRMCDADFAMITRQRDGVLFFAEAYGYSPEFVEHVRAL
                     PVERGRG"
     gene            complement(3083..4072)
                     /locus_tag="VM910_00570"
     CDS             complement(3083..4072)
                     /locus_tag="VM910_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61078.1"
                     /translation="MNRRDFITLLGGAPVAWPLAVRAQQPDMRRIGVLMNTAADEPEA
                     QARLTAFMQGLQESGWAVGRNLRVDYRWSPVPGDLARLQKDAAELVALRPEVVLAGVG
                     ATTAALQQATRTIPVVMAQGIDPVGNSFVESLARPGGNTTGFIQFEYSLAGKWMELLK
                     EVAPGVARVGVLREPAGAAIGQWAMIQAVAQSLGAELKPIDARDPDELERSIAAFARA
                     PNGGLIVAVSAAALTHRDLIITLAARYRLPTVYAYRVFVTYGGLMTYGPDILSQYRGA
                     AGYVDRILRGEKPADLPVQAPTKYELVINLKTARALGLEVPATLLARADEVIE"
     gene            complement(4069..>4284)
                     /locus_tag="VM910_00575"
     CDS             complement(4069..>4284)
                     /locus_tag="VM910_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61079.1"
                     /translation="AGGLMSYGASLPDLFRRGASYVDKILRGTRPAELPVEEPVKFDL
                     SINLKTAKAAGLTIPESFILRADEVIE"
BASE COUNT          783 a         1341 c         1305 g          855 t
ORIGIN      
        1 cccgcgtccc cgctcgaccg gcaaagccct gacgtgctcg acgaactcgg gcgaataccc
       61 gtaagcctcc gcgaagaaga gcaccccgtc tctctgccga gttatcatgg cgaagtcggc
      121 gtcacacatg cggccggctg actcaacgag agtttgtagc accgccttga gatcgaaggt
      181 tgagcggctg atgaccttga gcacgtccgc agtggcggtc tgctgttgca gcgattcgct
      241 cagttcgcga gtacgttgtt gcacttcgtc gaacagccgc acgttctcga tcgcgatcac
      301 cgcttggtcg gcgaaggact tgatgagcgc gacctccctg tcatcgaaga ccctgggttc
      361 gtgatgcgcc gtgccgatag cgccgatcac cttgtcgcct cggaccatgg gcgcatagat
      421 catcgaatcg taaccgaatt cctgggcgaa ctgcctggtg gcggacggtg ctttggggtt
      481 gccgcgcagc ggcgagtatt gaacgacctg gcgcgcaagc atcgtccgac cggcagccgt
      541 ggtgtcatcc agcgggcgcg ggaaccgctt gagtattttc tcaaaccccg gcgcaccgtc
      601 ggcggcgggc atttcaatga tgccgtcgtt caacagctgg acaccagcaa agcgcgttcc
      661 gaacagccgc agcaggttgc gcacgatcgc gtcgaacacg ggcttggggt ccgtcattga
      721 gccgctgatc gaagcgagaa tctcgcctgt cgcggtctgc cgctccagcg cctccttggt
      781 ctcttcgaac agccgcacgt tctcgatcgc gatcaccgct tggtcggcga aggtggtgac
      841 cagctcgatc tgcttgtcgc tgaaggggcg caccgagtta cgcgctagcc cgaacacgcc
      901 gattggcgtg ccttcgcgca tgaggggcat gccgagcaca gttcggaatc cgccgattct
      961 ctggacttcg atcaaagcgt aattcggctc ggccagaaca tcagccacct ggacggcccg
     1021 agcttcgagc agagctcgac cgatcacgct ctcacgcccc gctttgattg gcgtgcgctc
     1081 cagcacctcc ttgaactccc tcgagtagcc gaagtacgcc tgggtgtgaa agaggcccgc
     1141 ctcgttcggg cgagcgatat gtccgatgtc cgcctcgcaa aggcggacgg ccgactcgac
     1201 taacgtatcg agcactacct gcaaatcgaa cgtcgagcgg ctgataacct tgagcacatc
     1261 ggcagtggcc gtctgctgtt ggagagactg gcgtagctcc gataggagac gcgcattctc
     1321 gatcgcgatc accgcctggt cggcgaaggt ggtcacaagc tcgatctgct tgtcggtaaa
     1381 ggagttcact ttaacgcggg ttagcgtgaa ggtaccgatc gttgttccct cgcgcaggag
     1441 cggaacagcc agaatggttc tgtatcccag cgcctgctga tatccggttg cttcatactc
     1501 aggatcggcc agcacgtcgg gaatatgaaa cggcctgctt tctaacgccg ctcgcccggt
     1561 cacactgcca cgctccggcc gcagaggatg ctccagcgca taccgctccg cttcgcgtga
     1621 atacccataa ttcgacgcca gtctatagac gtctccgtcc cgctgcatga cggcgcccct
     1681 gtcagcctcg caaagtcggg cagccgatgc ggtcagcgta tcgagcacag attgtagatc
     1741 gaatgtggaa cggctgatga ctttgagcac gtcggcggtg gccgtttgct gctgcagtga
     1801 ttgccgcaac tcattaagca accgcgtatt ctcgatggcg atgacggcct gggctgcgaa
     1861 gtttgtgacg agttcgacct gcttgtcggt gaatgggcgg acctgctggc ggtagataac
     1921 aatggcgcca accaactcgt tttctttgac catggggacg gcaagcactg tccgggcccc
     1981 tgagagcttg gcaaactgtg ctccagttgt gcccgtcggg gcgttggcgt aacccggctc
     2041 gtcctggaca tcgccgattt gaactgcccg cttcgtcgcc aacgcgcgtc cgagcgttgt
     2101 ttcggggtta gggcggacca ttggatcgcg ccgccgcgct tcggcaaacg ctggcggtgc
     2161 accgtacatg gcaatcgtac gaagggcatc gccttcgcag aggtagagtg ttccgaactt
     2221 cgcctcgcag atgcgcactg cgttcgccag catggcctgg aacactggct ccagttcacc
     2281 aggcgagctg ctgatcacgc gcaacacctc agaggtcgcg gtctgctgtt gcagcgattc
     2341 gcgcagttca ttgagcagcc gcgcgttctc gatggcgatg acggcttggt cggcaaacgc
     2401 cttgacaagc tcgatttctc ggtcggagaa gagcgcgacg tgcccacgat agagagcgat
     2461 tgcacctatt ggatttccgt cacgcagcat cggcacgccg agataggtac gaatgccgat
     2521 ggaggctcgg taagctgcgg cgaaagtgta ccttggctcc tgttcgacat cgggaatgtg
     2581 aacgattttg cgttccaaga ccgttcggcc cgtagccgtt ctattgtctg gttgcatggg
     2641 ccagattttt tgatacgcat cgagcgcggt ctggtcgagg ttgtagtggg ccaccacgct
     2701 gagcagttcg ccgtcgtacc gaaagaccat accgtacgtg ggctccgcaa agatcgacgg
     2761 cactgcgcgc gatcgtatcg aagaccggct gcaggtcact gtgcgagctt gagattacgc
     2821 gtaggatttt gctcgtcgcc gcctgctgtt cccgcgcctc ggtgagcttc tttttgaggt
     2881 cggcattggc tgcgcgaagg cggtcaacgt cagtcgcctt cgttgtcttc gaacgaagac
     2941 cggtaatgcg tcttcggctc tttgcccctt tgcgagacaa ggtcacctcg tcggcacagt
     3001 cactggtccc cccttccggt tggcccagcc aaggatcgag cctgtcaatc ctaacagaac
     3061 cgcacgatga cctcgcgcct cttcactcga tcacctcatc ggcgcgggcg agcagcgttg
     3121 ccggcacttc aaggccgagc gctctcgcgg tcttgagatt gatcaccagc tcgtacttgg
     3181 tgggcgcctg caccggcagg tcggcgggct tctcgccgcg caagatgcga tcgacgtacc
     3241 cggccgcgcc ccggtattgg ctgagaatgt cggggccgta ggtcatcaac ccgccgtagg
     3301 tgacgaacac gcgataggcg taaaccgtcg gcagccgata gcgggccgca agcgtaatga
     3361 tcaagtcgcg atgggtcagc gcggccgcac tcacggccac gatcaggcca ccatttggag
     3421 cacgcgcgaa cgctgcgatg gaccgttcga gttcatcagg atcacgcgca tcaatcggct
     3481 tcaactccgc acccaatgac tgagctacgg cctggatcat tgcccattga ccgattgccg
     3541 caccggcagg ctcgcgcagg accccaacgc gcgctacgcc cggcgccacc tctttcagca
     3601 gctccatcca ttttcctgcc aagctgtatt cgaactgaat gaacccggtg gtgtttccac
     3661 ccggccgcgc caagctctcg acaaagctgt ttccgaccgg gtcaatgcct tgcgccatga
     3721 caaccggaat ggtgcgcgtc gcttgttgca gcgcggccgt ggtcgcgcca acgccggcga
     3781 ggacgacctc gggccgcagc gcaaccagtt cggccgcgtc cttctgcaag cgggcgagat
     3841 cgcccggcac gggactccag cgataatcga cgcgcagatt gcgtccgacc gcccaacccg
     3901 attcctgaag cccctgcatg aaggcggtga gccgcgcctg cgcctcgggc tcgtcggcag
     3961 cggtattcat gagcacgccg atgcgccgca tgtccggctg ctgcgcgcgt acggcgagcg
     4021 gccatgccac cggtgcgccg ccgagcagcg tgatgaaatc gcgccggttc actcgatcac
     4081 ctcgtcggcg cgcagaatga acgattccgg gattgtcagc ccggccgcct tcgcggtctt
     4141 taggttgatg ctcaggtcaa acttgaccgg ctcctccacg ggcagttcgg cgggcctagt
     4201 gcctcgcaaa atcttatcta catacgaggc tccgcgccga aacaagtcgg gtaggctggc
     4261 gccgtaggac atgaggcctc cggc
//
LOCUS       DATLJT010000027         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2298782,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000027 DATLJT010000000
VERSION     DATLJT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298782"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VM910_00580"
     CDS             complement(<1..774)
                     /locus_tag="VM910_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HVI61080.1"
                     /translation="MQPNSIAAWLTQARELVRRHRLQLILALRVTVAALSALALAQAL
                     HLRLPLWAVLTSLIVTQMSLGRSVKVATDYLLGTFGGVAYGGALAILIPHDNEWALLA
                     VLALAVAPLAFIAAFRANFNVLPVTAIIVLLVPSMQHVSPAVSALDRVLEVTVGGVVG
                     FIVSFLVWPSRAHEMTTDAAADTLELMAKALSKLLEDHRRGLERAALRRLNDGIGAAL
                     VRLNAFGAEAEHERNARLTSGPDTGPLLRTLLRLRHDIVI"
     gene            complement(808..1233)
                     /locus_tag="VM910_00585"
     CDS             complement(808..1233)
                     /locus_tag="VM910_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61081.1"
                     /translation="MSTKSRQKAFSGRVIGARIRADAAREAARKAVREADRAEAEAWS
                     VRMEGYGGPAQPSPTIAQCLNGGLAWLEVECNRCKTRASLPLDAIRRPRETPLWKLEA
                     SLKCRACRKGRHAPPVHMIRLTEQREITPYLWVHPDEER"
     gene            complement(1358..2527)
                     /locus_tag="VM910_00590"
     CDS             complement(1358..2527)
                     /locus_tag="VM910_00590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVI61082.1"
                     /translation="MLQRPDDGTPIATKAERYVPLDRARTFITLLVVLYHSVINYTYY
                     GIGGDRMRWLGFDLVVLFCDSFFMAAMFFISGLFVHASLTRRGAANYLASRAFRLGVP
                     FLASIFVVMPIAYYRYYAAKLDFAEYYWQMLTIGPWSAGSSWFLWVLLVFGAVGAMVW
                     AAAPHAIDALGRRVDALRDRPMTAFGAFLIFTIIVYLPSHLIFGDSSWLTPGHYPFPI
                     QTSRILLYAGYFFCGVAVGAVSLRSGMLAEDGELAKRWTTWLGSALICYGAILVLVYV
                     HRGGFVDLKAPPLWWRTAYGLAFAMFAAAMTFTVPAVFLRFALSSFRLLDAMQPQAYG
                     IYLVHFMPLIWLQYLIYDPPYPAFAKFAIVFAGTLSLSWALTFLLRRLPVLADMI"
BASE COUNT          583 a          865 c          932 g          429 t
ORIGIN      
        1 aatcacgatg tcatgacgca accgaagcag ggtgcgcagc agcggtcctg tatcgggacc
       61 ggaggtgagg cgcgcgttgc gctcgtgctc ggcctcggcg ccgaacgcgt tcaatcgcac
      121 cagcgctgcg ccgatcccgt cattgaggcg gcggagagcg gcccgttcca gcccgcgcct
      181 gtgatcctcg agcagctttg acagcgcttt cgccatcagc tcgagggtgt cggcggcggc
      241 gtcggtcgtc atctcatgcg cgcgggaggg ccagaccaga aacgacacga tgaagccgac
      301 gacgccgccg acggttacct ccagcacgcg atcgagcgcg gagacggccg ggctgacatg
      361 ttgcatgctc ggcaccagca gcacgatgat tgccgtcacc ggcagcacgt tgaaattggc
      421 ccgaaaggcc gcgatgaagg cgagcggcgc tacggcgagc gcaaggaccg caagcagcgc
      481 ccattcgttg tcgtgcggga tgaggattgc gagcgccccg ccataggcga cgccgccaaa
      541 cgttcccagc agataatcgg tcgccacctt cacggagcgg ccgagcgaca tctgggtcac
      601 gatcagcgat gtcagcaccg cccagagcgg caggcgcagg tgcagcgctt gagcaagcgc
      661 cagcgccgag agcgctgcga ccgtgacccg cagcgcgaga atgagctgca gccgatggcg
      721 gcgcacaagc tcgcgtgctt gcgtcagcca tgcggcgatg gagttgggct gcattttcgt
      781 cgtggcctca agccgtgtgc aaaggtttca ccgctcctcg tccggatgca cccagagata
      841 gggcgtgatc tcgcgctgtt cggtgaggcg gatcatgtgc accggcggtg cgtgacggcc
      901 cttgcggcag gcgcggcatt tcagcgaggc ttcgagcttc cacagtggcg tctcgcgcgg
      961 ccttcggatc gcatccagcg gcaggctcgc gcgcgtcttg cagcggttgc attcgacttc
     1021 gagccaggcg aggcctccgt tgagacattg cgcgatggtc ggcgagggct gcgcgggccc
     1081 gccatagcct tccatgcgga ccgaccaggc ttcggcctcc gcgcggtcgg cctcgcggac
     1141 ggctttcctt gcagcctcgc gcgcggcatc ggctcggatc ctggcgccga tgaccctgcc
     1201 gctgaaggcc ttctggcggg atttcgtgct catcgtcccg attacagcgc cgcgggcggc
     1261 caagcgcaac gccccagtga tggggtgagg gtcgggccgg ccccaccagc gcttcggcat
     1321 ggcgattttg tccacaacag gtgcgcaggg cagaccgtca gatcatgtcg gccaggaccg
     1381 gaagccgtcg caagagaaat gtcagtgccc agcttagcga cagcgtgccg gcgaaaacga
     1441 tcgcgaattt cgcaaaagcc gggtagggcg gatcgtagat gaggtattgc agccagatca
     1501 gtggcataaa gtgtacgaga tagatgccgt aggcctgcgg ctgcatcgca tcgagcagcc
     1561 gaaacgagga tagcgcaaag cgcaggaaga ccgccggaac cgtaaacgtc atggcggctg
     1621 cgaacatcgc gaaggcaagg ccataggccg tacgccacca gagcggcggc gctttcagat
     1681 cgacgaaccc gccgcgatgg acatagacga ggaccaagat cgcgccgtag cagatcagcg
     1741 cgctgccaag ccaggtagtc cagcgcttcg ccagttcccc atcctccgca agcatcccgg
     1801 acctcaaact gaccgccccg acagcgacac cgcaaaagaa atagccggca taaagcagga
     1861 tgcggctggt ctggatcgga aacgggtagt gccccggcgt gagccaggat gaatcgccga
     1921 agatcaggtg cgacggcagg tagacgatga tcgtgaagat caggaacgcg ccgaacgcgg
     1981 tcatcggccg gtcacgcagg gcgtcaacgc gtcgtccgag cgcgtcgatg gcgtgcggag
     2041 ctgccgccca caccattgcg ccgaccgcgc cgaagacgag caggacccac aggaaccacg
     2101 acgatcctgc cgaccaggga ccgatggtca gcatctgcca atagtactcg gcaaaatcga
     2161 gcttcgcggc gtaatagcgg taataggcga tcggcatcac cacgaagatc gaggcaagaa
     2221 acggcacacc gagacgaaag gcgcggcttg caagataatt cgcggcgccc cggcgcgtca
     2281 ggcttgcgtg cacgaacagt ccggagatga agaacatggc ggccatgaag aagctgtcgc
     2341 agaacagcac gacgagatcg aagccgagcc agcgcatgcg gtcgccgccg atgccgtaat
     2401 aggtataatt gatgacggaa tggtagagca cgacgaggag ggtgatgaag gtccgcgcgc
     2461 gatcgagcgg aacgtatcgc tccgcctttg tcgcgattgg cgtgccgtca tcgggccgtt
     2521 gcagcatggg ctgaagctac gtccccggac ctcgtggcgc cagtgctccc gagcttcgaa
     2581 agagggcacc ggtgcaacgc actgatttgc ccgacaagcg gaacgcaaag tgcgcatgcg
     2641 tgttatgcgc aaattgcccg tcgtgccaat ctgccgcagt tccgattttc gaaaatcatg
     2701 ttgaccgaaa ccccaaatca aaacgatcat ccgcgccatc ccgcctctgc aagaggggcg
     2761 cttcgcgatc gtcacgagtg ttggaagcgg gatgcgatgg acgcgacac
//
LOCUS       DATLJT010000028         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3447490,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000028 DATLJT010000000
VERSION     DATLJT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447490"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VM910_00595"
     CDS             <1..1153
                     /locus_tag="VM910_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVI61083.1"
                     /translation="MVVMDPWTGRVLAMVGGFSFDQSQFNRATQAYRQPGSSFKPIVY
                     SAALDNGYTPSTVVMDAPIEIDQGQGAGVWRPENFSSGKFQGPTTLRNALRLSLNTVT
                     VRLAQDIGMPLISEYARRFGVYDELPNYLSYALGAGETTVMRMVTAYSMIANGGRRVK
                     PTLIDRIQDRYGHTIFKHDARECRGCDAPGGWKSQPEPQLVDRREQVLDAMTAYQMTS
                     LMEGVVQGGTGTALKEVGKPIAGKTGTTNEAKDAWFIGFSPDIVVGIYMGYDKPRPLG
                     KGNAATGGHLAAPIARDFMKLALADKPPTPFKIPAGIKLIRVDAKSGMRVGPGEGGRG
                     VILEAFKPGTAPPDNYSVIGVADADRVIMPQGTSPDAGNIMRPGTGGLY"
     gene            1252..2383
                     /gene="prfB"
                     /locus_tag="VM910_00600"
     CDS             join(1252..1320,1322..2383)
                     /gene="prfB"
                     /locus_tag="VM910_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVI61084.1"
                     /translation="MRAEIERLVEEIKQSVGLLRRHLDVERSTARLAELNKLAEDPNL
                     WNDPQKAQKLMQERTSLEDALSGIGKVERELQDNIDMIELGEAENDAGVVAEAEEALK
                     DLKKEVARRELEALLSGEADRFDSYLEVHAGAGGTESQHWAEMLLRMYTRWAEKHGFK
                     IEYLEESQGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSV
                     QVFPVIDDTIKIDIKDSDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVCQAGR
                     SQHKNRAQAWDMLRARLYEIELKKREEKAAAEQAAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVQTSDTSGVLDGDLDEFMAATLAQRAFGTTADAVEDVE"
     gene            2387..>3226
                     /locus_tag="VM910_00605"
     CDS             2387..>3226
                     /locus_tag="VM910_00605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI61085.1"
                     /translation="MGHVAFIGLGRMGHGMAGRYLDAGFRLAVWNRSKEKAEDLIARG
                     AQWATSPEDAAIDADAVVTMVADDEASRSVWLGKNGAAATMKAGTLAIECSTISYAHA
                     LDLSRELDNRGLVYIDSPVTGLPDAAASGKLTLLVGAHPADLEKARPFLEPLSSTIRH
                     FGAVGTGTVYKLINNLMGAIQIAGIAEGLAIAEQAGLDMKLVLEAIESGVAASPQVIR
                     HSRRMAARNFAGATFTAALRHKDAAYAVALAESLLSSEPLIGRAAVEAYARAKAAAPD
                     DDEA"
BASE COUNT          658 a          983 c         1032 g          553 t
ORIGIN      
        1 catggttgtt atggacccct ggaccggacg cgtgcttgcg atggtcggcg gcttctcctt
       61 cgaccagagc cagttcaacc gcgcgacgca ggcctatcgg cagccgggtt cctcgttcaa
      121 gccgatcgtc tattcggcgg ctctggacaa cggctatacg ccctcgacgg tggtaatgga
      181 cgcgccgatc gaaatcgatc aagggcaggg tgccggggtc tggcgtccgg aaaacttctc
      241 ctccggcaaa ttccaggggc cgaccacgct gcgtaacgca ctcaggctgt cgctgaatac
      301 ggtgactgtg cggctggcgc aggacatcgg gatgcccttg atcagcgaat acgcgagacg
      361 cttcggcgtt tacgacgaat tgccgaatta tctgtcctac gcgctgggcg ccggcgaaac
      421 cactgtcatg cggatggtca ccgcttactc gatgatcgcc aatggcggaa ggcgtgtgaa
      481 gccgaccctg atcgaccgca tccaagaccg ttacggccat accatcttca agcatgatgc
      541 tcgcgaatgc cgcggctgcg acgcgcccgg cggctggaaa agccagccag aacctcagct
      601 cgtcgaccgc cgcgagcagg tgcttgatgc catgactgcg taccagatga cctcgttgat
      661 ggagggcgtt gtgcagggcg gtaccggcac tgcgctgaag gaagtcggca agccgatcgc
      721 cgggaaaacc ggcaccacca acgaggcgaa ggacgcgtgg ttcatcggct tctcgcccga
      781 tatcgtggtc ggcatttaca tgggctatga caagccgcgt ccgctgggca aaggcaatgc
      841 cgcgaccggc ggtcacctcg ctgctcccat cgcccgcgac ttcatgaagc tcgcgttggc
      901 cgacaagccg ccgacgccgt tcaagattcc ggccggcatc aagctgatcc gtgttgacgc
      961 caagagcggc atgcgggtcg gtccgggcga gggcggacgc ggtgtcatac tggaagcgtt
     1021 caagccgggt acggcgccgc cggataatta ctccgtcatc ggcgttgccg atgcggacag
     1081 agtcatcatg ccgcagggaa cgtcgccgga cgccggcaat atcatgcggc cggggaccgg
     1141 cgggctctac tgatcaacgg cgtctcgacg aagacggcgg ggccgattgc actttcggct
     1201 ccgcgccgct acaccctctg accatattgc aagcgatcac ccgggaacac catgcgcgcg
     1261 gaaatcgaac ggttagtcga agagatcaag cagtcggtcg gactgctgag gaggcatctt
     1321 tgacgtcgag agatcgacgg cgcggctggc tgagctgaac aagctcgccg aagaccccaa
     1381 tctctggaac gatccgcaaa aggcccagaa actgatgcag gagcgcacct cgctggagga
     1441 tgcgctgtcg ggcatcggca aggtcgagcg cgagcttcaa gacaatatcg acatgatcga
     1501 gctcggcgag gccgagaacg atgccggcgt cgttgccgaa gccgaagagg cactgaaaga
     1561 tctgaagaag gaggttgcgc ggcgcgagct ggaagcactc ttgtcaggcg aagccgaccg
     1621 cttcgattcc tatctggaag tccatgccgg cgcgggcggt accgaaagcc agcactgggc
     1681 ggagatgctg cttcgcatgt acacccgctg ggccgagaag cacggcttca agatcgaata
     1741 tctcgaggag tcccagggcg aagaggccgg catcaaatcg gcgaccatcc agatctcggg
     1801 ccataacgcc tatggctggc tgaagacaga agccggcgtg cacaggctcg tacgcatctc
     1861 gccgtttgat tccaacgcgc gacggcacac ctcgttttca tccgtgcagg tcttcccggt
     1921 catcgacgac acgatcaaga tcgacatcaa ggattcggac gtgcgggttg ataccatgcg
     1981 ttccggcggt gccggtggcc aacacgtcaa caagaccgaa tccgcggtgc gtctcaccca
     2041 tatcccgacc ggcatcgccg tggtctgtca ggccggccgt tcccagcaca agaatcgcgc
     2101 ccaggcctgg gacatgctgc gggcacggct ctatgaaatc gaactgaaaa agcgcgagga
     2161 gaaggctgcc gccgagcagg ccgccaaaac cgatatcggc tggggccacc agatccgctc
     2221 ctacgtgctg cagccctatc agatggtgaa ggatttgcgc acgggcgtgc agacctccga
     2281 cacttcaggc gtgcttgacg gcgacctcga cgaattcatg gcggcgacgc tcgcacaacg
     2341 cgccttcggc accaccgcgg atgcggtcga ggacgtggaa taaatcatgg ggcatgtcgc
     2401 cttcatcggt ctcgggcgaa tgggtcacgg catggccggc cgttatctcg atgccgggtt
     2461 caggctcgcg gtatggaatc gcagcaaaga aaaggccgaa gacctgattg cgcgcggcgc
     2521 gcaatgggcc acctcgccgg aggacgctgc aatcgacgcc gatgcggtcg tgaccatggt
     2581 cgccgacgac gaggcctcgc gctccgtctg gcttggcaag aatggcgcgg ccgcgaccat
     2641 gaaggccggt acgcttgcga tcgaatgttc gaccatctcc tatgcccacg ctctcgatct
     2701 gtcgcgagaa ctcgacaacc gcgggctggt ctatatcgat agcccggtga cgggcctgcc
     2761 ggatgccgcg gcaagcggaa agctgacgct tttggtgggt gcccatccgg ccgatctcga
     2821 aaaggcacgg ccgttcctcg aaccgctgtc atccacgatc agacatttcg gtgccgttgg
     2881 aacaggcacg gtctacaagc tcatcaacaa tctgatgggt gcaatccaga ttgcgggcat
     2941 tgctgagggg ctcgcgattg ccgagcaggc cggcctcgac atgaaattgg tgttggaggc
     3001 aattgaaagc ggcgttgccg ccagccctca ggtgatacgg cattcgaggc gaatggctgc
     3061 gaggaacttt gcgggcgcga cctttaccgc ggcgcttcgc cacaaggatg ccgcctatgc
     3121 cgtggcactc gccgaaagcc tgctttccag cgaaccgctc atcggacgcg cggcagtcga
     3181 agcctatgcg cgcgccaagg cggctgcgcc ggatgacgac gaagcc
//
LOCUS       DATLJT010000029         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1273,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000029 DATLJT010000000
VERSION     DATLJT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..597
                     /locus_tag="VM910_00610"
     CDS             178..597
                     /locus_tag="VM910_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61086.1"
                     /translation="MAKLAQGTVCKILAEHEVKPHKVRYYLEKRDPEFEPKMAEILCV
                     YRQVAMLRGQAQSGASQDPDGGGGGGLAIVSYDEKPGIQAIATTAPDRPPLPGTSPTV
                     MRDHEYKRHGTLTLMAGIDLFTGHAGATSRRGRRRAD"
     gene            825..2204
                     /locus_tag="VM910_00615"
     CDS             825..2204
                     /locus_tag="VM910_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HVI61087.1"
                     /translation="MQTECSADLFGFAPVEGRKVVAGFDGGRMTSDAGAMLLGAADKR
                     IGLIERVAGCFTDYRDADLIEHTVAGLVGQRVFGIALGYEDLIDHEQLRHDPVMAVLG
                     GKLEARRADCAPLAGKSTLNRLELSREEPTRYHKISYDAAAIEGLFVDLFLAAHPAPP
                     AQITLDLDATDDPLHGHQEGRFFHGYYDTYCYLPLYVFCGRHLLAAKLRPANIDASAG
                     SVEEIARIVAQIRRRWPKVRILLRADSGFAREALMTWCENNDVDFLLGLAKNSGLVGE
                     IEAELAAAQEHSQRTEKPARRFKDFTWQTRESWSRARRVVAKAEWTRGEANPRFVVTS
                     LAREEHEARHLYEKLYCARGEMENRIKECQLELYADRTSAHTMRANQLRLWFAAMAYV
                     LICAVRRIGLKHTQFARAACGTIRLKLLKIGALVRISVRRIKLAMPSAFPYQTEYRAA
                     HAALTAAAA"
BASE COUNT          472 a          754 c          763 g          399 t
ORIGIN      
        1 gggtattttt cggccgcgtg ccggacgcga tcggatcatc accgctgagg cgaagacctg
       61 gctggtggca ttggcgtgcc ggaaggccga ggaattgggc cacccccacg aactgtggac
      121 cacccggctg ctcgccgcgc acgcccgcca gcacgggccg agcgccggca tccgagcttg
      181 gcaaagctgg cccagggaac ggtttgcaag atcctggccg agcacgaggt caagccgcac
      241 aaagtgcgct attacctgga aaagcgcgat cccgagttcg agcccaagat ggccgaaatc
      301 ctctgtgtct accggcaggt ggcgatgctg cgtgggcaag cgcaaagtgg ggcgagccag
      361 gacccggatg gcggcggtgg cgggggtctc gccattgtct cttacgacga gaaacccggg
      421 atccaggcga tcgctacgac ggcgccggat cgcccacccc ttcccggcac aagcccgacc
      481 gtgatgcgcg atcacgaata caagcggcac ggcactctga ccctgatggc gggtatcgat
      541 ctgttcaccg gtcacgccgg agcaacctcg agaagaggtc gcaggagggc ggactagaca
      601 gccagccacg tcccggaagc accgcgacga gccctgcgaa gagcgcaaga aagacagcga
      661 cgcggaccac cgggatcaat cctgccagca gctgttgaat aggcccgagg cgttatcatg
      721 aaacggctaa cccggatttc tcaaacgggg caggtgcatc gggaccctgg atctgagaaa
      781 atccagttga cagaacggtt tatctcagga ccaggggagt cccgatgcag acagagtgta
      841 gcgcggatct gtttggattt gcacccgtcg aagggcgcaa agtggtggcc gggtttgatg
      901 gcggccggat gacctcagat gccggcgcaa tgctgctcgg cgccgccgac aagcggatcg
      961 ggctgatcga gcgcgttgcc ggctgtttca ctgactaccg ggacgccgat ctgatcgaac
     1021 atacggtggc gggtctggtc gggcaacggg tgttcgggat tgcgttgggt tacgaggacc
     1081 tgatcgacca cgagcaattg cgccacgacc cggtgatggc ggtgctcggc ggcaagcttg
     1141 aagcgcggcg cgcggactgt gccccattgg ctggcaaatc gacgctcaac cggttggaat
     1201 tgagccggga ggagccgacc cgttatcaca agatcagcta tgacgcggcg gcgatcgagg
     1261 gcctgtttgt cgacctgttt cttgcggcgc atccggcgcc gccggcgcaa atcaccctcg
     1321 atctcgacgc caccgacgat ccgctgcacg gccatcagga agggcgtttc ttccatggct
     1381 attacgatac ctattgctat ctgccgctct atgtgttctg cggccggcat ttgctggccg
     1441 ccaaattgcg cccggccaat atcgacgcca gtgccggcag cgtcgaagag attgcgcgga
     1501 tcgtcgccca gatccgccgg cgctggccca aggtgcgcat tctgctgcgt gccgattcgg
     1561 gctttgcccg cgaggcgctg atgacctggt gcgagaacaa cgacgtcgat ttcctgctcg
     1621 gcctggccaa aaacagtggg ctggtcgggg agatcgaggc cgagctcgcc gccgcgcaag
     1681 agcacagcca aaggaccgaa aagccggccc gccgcttcaa ggacttcacc tggcagacgc
     1741 gcgagagctg gagccgcgcg cggcgggtcg tggccaaggc ggaatggacc cgcggcgaag
     1801 ccaacccgcg ctttgtcgtc acctcgctgg cgcgcgagga gcatgaggcc cgccacctct
     1861 atgagaagct ctactgcgcc cgcggcgaga tggaaaaccg gatcaaggag tgccagctcg
     1921 agctttatgc cgaccgcacc tcagcccaca cgatgcgcgc caaccagctg cggctgtggt
     1981 tcgccgcgat ggcctatgtg ctgatctgcg ccgtgcgccg catcggcctc aagcacaccc
     2041 agttcgcccg cgccgcgtgc ggcaccatcc gcctcaaact gctgaagatc ggggcgctgg
     2101 tgcgcatcag cgtgcgccgc atcaagctcg ccatgccctc tgcctttccc tatcagaccg
     2161 agtaccgcgc cgcccatgcc gcgctgaccg cggcggcagc ctgacacgaa ctggaaagca
     2221 cgacaaaaaa ataatcctca cccgctcaaa ggatcactgc cctcaatagg actcgctcaa
     2281 gttgaaatct gtctcgttta cccgcaaccg ttatcgcgtt cggctgctct gttgagctgc
     2341 gccttgctgc cacttaaaaa tcaaaccggg tatgagaaat ccgggcta
//
LOCUS       DATLJT010000030         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_231027,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000030 DATLJT010000000
VERSION     DATLJT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231027"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2870)
                     /gene="polA"
                     /locus_tag="VM910_00620"
     CDS             complement(<1..2870)
                     /gene="polA"
                     /locus_tag="VM910_00620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVI61088.1"
                     /translation="MPKSPQKAAAKPAAAEKQVPAKTPVAAEGAGKGDHVFLVDGSSY
                     IFRAYHALPPLNRKSDGLQVNAVLGFCNMLWKLLRDMPENEKPTHLAIVFDKSEVTFR
                     NKLYPDYKAHRPPAPDDLIPQFALIRDAVRAFDLPCLEQNGFEADDLIATYARLATTR
                     GATTTIVSSDKDLMQLVTDKVTMYDTMKDRRIGRAEVIEKFGVPPEKVVEVQALAGDS
                     TDNVPGVPGIGVKTAAQLIVEYGDLDAILFRATEIKQPKRREALIENAEKARISRQLV
                     LLDDKVDLEVPLDDLAVQEPDARKLIAFLKAMEFSSLTRRVAEFSQIDASQIGAAMPS
                     SSHLRAPSNGEAAQEGSTSGAPPSATQKAGAAAPGKGTGDRSDKSASLKGTPQALAEA
                     RADTARKTPVDRAKYQTIRNLDQLKAWAARARDAGLFAIEAKSSSIDPMQSEFSGIAL
                     ALGVNDACYVPLSHKQAGDGVGLFDAGLAPDQIKESEALDVLRPLLQSAGILKIGFNI
                     KFGAVMLAQAGISIRNYDDVQLMSYALDAGRNAHGLDSLAERWLGHTTLTHGELTGSG
                     KNKLSFDQVTIDRATAYSAEDADVILRLWRLLKPRLVAERMMTVYEMLERPLVAVLAR
                     MERRGISIDRQVLSRLSGDFAKTAARVEAELQELAGEPINVGSPKQIGDILFGKMGLP
                     GGTKTKTGAWSTSAQVLDDLAEQGYEFPRKILEWRQVSKLKSTYTDALPTYVNPQTHR
                     VHTTYALAATTTGRLSSNEPNLQNIPVRTEDGRKIRRAFIATPGHKLVSADYSQIELR
                     LLAEIADVPMLRQAFRDGLDIHAMTASEMFGVPIKDMPSEVRRRAKAINFGIIYGISA
                     FGLANQLGIAREEASAYIKKYFERFPGIRAYMDETREFCRKNGYVTTLFGRKCYYPEI
                     RASNASIRSFNERAAINARLQGSAADIIRRAMTRMEDALAEK"
     gene            complement(3054..4094)
                     /locus_tag="VM910_00625"
     CDS             complement(3054..4094)
                     /locus_tag="VM910_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVI61089.1"
                     /translation="MTANGTSATKRVDWVDYAKGICIVMVVMMHSVLGVEQAAGQTGF
                     MHALVAFAKPFRMPDFFLISGLFLNLVIDRDWRTYLDRKVVHFAYFYVLWVTIQFGFK
                     APAFAAESSWSHVGLLYLESFIEPFGTLWFIYLLPIFFVVIKLTRRVSPGLIWGAAAL
                     LEAAHVSTGWTVIDESCARFVYIYSGYLFADYVFALSDRARAYPFAAIAALLAWALVN
                     GTLVARGFSEWPVISLMLGLCGACAIVTIGTLLARMEWLNILRFCGEHSIVIYLAFFL
                     PMASTRTLLLKTGLISDIGTVSLIVTVVGVVGSLLIWRLALASGANFLFERPDAFWIA
                     PKKGPTALQAAE"
     gene            4264..>5192
                     /locus_tag="VM910_00630"
     CDS             4264..>5192
                     /locus_tag="VM910_00630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HVI61090.1"
                     /translation="MRIAMIGTGYVGLVSGACFADFGHRVTCVDKDADKIAGLHRGEI
                     PIYEPGLDALVASNVKARRLDFTTNLSAPVAEADAVFIAVGTPSRRGDGYADLTYVYA
                     AAREIAAALSGFTVVVTKSTVPVGTGDEVELIIRETNPAADVVVASNPEFLREGAALR
                     DFKFPDRIVIGTSDERGRKVLSDIYRPLSLNQAPLMFTKRRTAELIKYAANAFLATKI
                     TFINEIADLSEKVGADVQEVARGIGLDNRIGSKFLHAGPGFGGSCFPKDTRALVQIAR
                     DHDVQLRIVEAVLGVNDNRKRAMARKVSSAAGGS"
BASE COUNT          959 a         1563 c         1674 g          996 t
ORIGIN      
        1 tttttctcgg cgagtgcatc ttccatgcgg gtcatggcgc ggcggatgat atcggcggcg
       61 gatccctgca gtcgcgcgtt gatcgcggcg cgttcgttga aggaccggat cgaggcgttg
      121 gaagccctga tctcggggta atagcacttg cggccgaaca gcgtcgtcac gtagccgttc
      181 ttgcggcaga actccctggt ttcgtccata taggcgcgga tgccgggaaa gcgctcgaaa
      241 tatttcttga tataggcgga agcctcttcg cgcgcgatgc cgagctggtt ggcgagcccg
      301 aacgccgaga tgccgtagat gatgccaaaa ttgatcgcct ttgcgcggcg ccgcacctcg
      361 ctcggcatgt ccttgatcgg cacgccgaac atttcggacg cggtcatcgc gtgaatgtcg
      421 agcccatcgc ggaacgcctg ccgcagcatg ggaacgtcgg caatctcggc aagcagccgc
      481 agctcgattt gcgaatagtc cgccgatacc agcttgtggc cgggcgtcgc gataaaggcg
      541 cggcggattt ttcggccgtc ctcggtgcga acagggatgt tttgcaaatt cggctcgttc
      601 gacgacagcc gtcccgtggt ggtcgcagcg agcgcgtagg tcgtgtgaac gcggtgggtt
      661 tgcggattga cataggtcgg cagcgcgtcg gtataggtcg atttcagttt cgagacctga
      721 cgccactcca ggatcttcct ggggaattcg tagccctgct cggcaagatc atccagcacc
      781 tgcgccgaag tcgaccacgc gccggtcttg gtcttggtgc cgccgggcaa acccatcttc
      841 ccgaacagga tatcgccgat ctgcttcggg ctgccgacat tgatcggttc gcctgcaagc
      901 tcctggagtt cggcttcgac ccttgccgcg gtctttgcga aatcgccaga caggcgcgac
      961 aggacctgcc gatcgatcga aatgccgcgg cgttccatgc gcgccagcac ggcaaccagc
     1021 ggccgctcca gcatttcata gaccgtcatc atgcgttcgg cgacaaggcg cggtttgagc
     1081 aggcgccaca gccgcaagat gacatcggcg tcctcggccg aataggcggt cgcccggtcg
     1141 atcgtcacct ggtcgaagga aagcttgttc ttgccgctgc cagtcagctc gccgtgggtc
     1201 agggtggtgt ggccgagcca gcgctccgca agcgaatcga gcccgtgcgc attgcgcccg
     1261 gcatccagcg cgtagctcat gagctgcacg tcgtcgtaat tgcggatgct gattccggcc
     1321 tgcgcgagca tcaccgcgcc gaacttgatg ttgaagccga tcttgagaat gccggcggat
     1381 tgaagcagcg gccgcagcac gtcgagcgcc tcgctctcct tgatctgatc cggtgccagg
     1441 cccgcatcga acaggccgac gccgtcgccg gcctgcttgt gtgaaagcgg cacgtagcag
     1501 gcgtcgttca cgccaagcgc aagcgcgatg cctgaaaatt ccgactgcat cggatcgatc
     1561 gagctcgatt tggcttcgat cgcgaacagg cctgcgtcgc gggcgcgtgc agcccaggcc
     1621 ttgagctgat cgagattgcg gatcgtctga tacttcgcgc ggtcgaccgg tgtctttcgc
     1681 gcggtatcgg cgcgcgcttc cgcaagcgcc tgaggcgtgc ccttgaggct tgcggacttg
     1741 tcgcttctgt cgcccgtccc cttgcccgga gcggctgctc ccgccttttg ggttgcggag
     1801 gggggtgcac cggaagtgct tccctcctgg gcggcctcgc cgttcgacgg cgcacgcaga
     1861 tgtgacgacg agggcatggc ggccccaatc tgcgaagcgt cgatttgcga aaattcggcc
     1921 acgcgcctgg tgagcgagga gaactccatc gccttcagaa acgcgatcag cttgcgtgcg
     1981 tccggttctt ggactgcgag atcgtccagc ggcacttcga gatcgacctt gtcgtcgagc
     2041 agcacgagtt gtcgcgaaat ccgcgccttc tcggcgtttt cgatgagggc ttcgcgccgc
     2101 ttcggctgct tgatctcggt ggcgcggaac aggatcgcat ccaggtcgcc atattcgaca
     2161 atgagctgcg cggcggtctt gacgccgatg ccgggcacgc ccggtacgtt gtcggtcgaa
     2221 tcgccggcga gcgcctgcac ctcgaccacc ttctccggcg ggactccgaa cttctcgatc
     2281 acctcggcgc gtccgatgcg gcggtccttc atggtgtcgt acatggtgac cttgtcggtc
     2341 acgagttgca tcaggtcctt gtcggaggag acgatggtcg tggtggcgcc gcgcgtcgtc
     2401 gcgagccgcg cataggttgc gatcagatcg tcagcctcga agccgttctg ctccagacag
     2461 ggcaggtcga aggcacgcac ggcatcgcgg atcagcgcaa attgcgggat caggtcgtcg
     2521 ggggccggcg gccggtgcgc cttgtagtcg ggatagagct tgttgcggaa ggtgacctcg
     2581 gacttgtcga agacgatggc gagatgcgtc ggcttctcgt tctccggcat gtcgcgcaga
     2641 agcttccaca gcatgttgca gaagccgagc acggcgttga cctgcaagcc gtcggatttg
     2701 cggttgagcg gaggcagcgc gtggtaggcg cggaaaatgt aggaggaacc gtcgaccaga
     2761 aaaacatggt cgcctttgcc cgcgccttcg gcggcgacag gggttttggc agggacctgc
     2821 ttttcggcag cggcgggttt ggcggcggcc ttttggggag atttcggcat ggccgcaatt
     2881 taagaatttt tgcggcgatt gacagtctcc ggaaggcgat ttttgccctg ctgacaggtc
     2941 tatccacgct gtcatcaccc gcgaaagcgg gtgatccagt accccagaaa tcgtcgtgat
     3001 tgaatggatc ggctgcggct tccggtcaag ccgggcgatg acagcagtga ggcttactcc
     3061 gccgcctgca atgcggtcgg acccttcttc ggtgcgatcc agaacgcgtc cggacgctcg
     3121 aacaggaagt ttgcgccgct agccagcgcc aggcgccaga tcagaagcga tccgacgacg
     3181 ccgacgacgg tgacgatcag cgaaaccgtg ccgatgtcgg agatcaggcc ggtcttcaac
     3241 aagagcgtcc gcgtcgaggc catcggtaga aagaaggcga gataaatgac gatggagtgc
     3301 tcgccgcaga agcggaggat gttcagccac tccatccgtg cgagcagcgt tccgatcgtg
     3361 acgatggcgc aggcgccgca aaggccaagc atcaacgaga tgacgggcca ttcgctaaag
     3421 ccgcgcgcaa cgagggtgcc gttgacgagc gcccacgcga ggagcgctgc gatcgccgcg
     3481 aacggataag cccgcgcacg atcggacagc gcgaagacat aatccgcgaa cagatagccg
     3541 gagtagatgt agacgaatcg cgcgcaggac tcgtcgatga cggtccagcc ggttgagaca
     3601 tgtgcagcct ccagcagcgc ggctgcgccc cagatcaggc cgggcgagac acgacgtgtc
     3661 agttttatga cgacgaagaa gatcggcagc agatagatga accagagcgt gccgaacggc
     3721 tcgatgaagg attcgagata gagcaagccg acatggctcc aactcgattc ggcagcgaag
     3781 gcgggggctt tgaagccgaa ctggatcgtc acccacagca cgtaaaagta agcgaagtgc
     3841 acgaccttgc ggtcgagata ggtacgccag tcgcggtcga tcacgaggtt gaggaacagc
     3901 ccggaaatca ggaagaagtc cggcatccga aacggctttg cgaaggcaac caacgcatgc
     3961 atgaagccgg tctgaccggc ggcctgctcc acgcccagga ccgaatgcat catcacgacc
     4021 atgacgatgc agatgccctt ggcatagtcc acccagtcca cgcgcttcgt cgcggatgtg
     4081 ccgtttgcgg tcatcggtgt ccctttatgc cgtgcgatcg cccatcatgg gtggcaagcg
     4141 gtttctttcc cctttattcg ttcaaggacc atgccgtttt ctccttcctt ttgcggccaa
     4201 atgctttaag aggcggttcg cattgaacag cggtcgcgtt aaccataagg ggcggatctt
     4261 tccatgcgga ttgccatgat tggcacgggc tatgtggggc tggtttccgg cgcctgcttt
     4321 gcggatttcg gccaccgcgt cacctgcgtg gacaaggatg cagacaagat cgcgggcctg
     4381 caccggggcg agattccgat ctatgagccg gggctggacg cgctggtggc atcgaatgtc
     4441 aaggcgagac ggctcgactt caccaccaat ctgtcggcgc cggtcgccga agccgacgcc
     4501 gtcttcattg cagtcggcac gccatcgcgg cgcggtgacg ggtacgccga cctcacctat
     4561 gtctatgccg ccgcgcgcga gatcgccgca gccctttccg gctttaccgt ggtcgtgacc
     4621 aaatcgaccg tgccggtcgg aaccggcgac gaggtcgagc tcatcatccg cgaaaccaat
     4681 ccggccgccg atgtcgtggt cgcatcgaat ccggaattcc tgcgcgaggg cgcggcgctc
     4741 cgcgacttca aatttcccga tcgcatcgtg atcggcacat ccgacgagcg cggccgcaag
     4801 gtgctgagcg acatctatcg tccgctctcg ctcaaccagg caccattgat gttcaccaag
     4861 cggcgtacgg cggaattgat caaatatgcc gccaacgcct ttcttgccac caagatcacc
     4921 ttcatcaacg aaatcgccga tctgtcggag aaggtcggcg ccgacgtgca ggaggttgcg
     4981 cgcggcattg gtctcgacaa ccgcatcggc tcgaaattcc tgcatgccgg ccccggcttt
     5041 ggcggctcct gcttcccgaa ggatacgcgc gcgctcgtcc agatcgcgcg cgaccatgac
     5101 gtccagctcc gcatcgtgga ggcggtgctc ggcgtcaacg acaaccgcaa gcgcgcgatg
     5161 gcgcgcaaag tgtcgagtgc ggccggcgga tc
//
LOCUS       DATLJT010000031         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2298897,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000031 DATLJT010000000
VERSION     DATLJT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298897"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..388)
                     /locus_tag="VM910_00635"
     CDS             complement(86..388)
                     /locus_tag="VM910_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61091.1"
                     /translation="MPAVEENATNERPRRNEVCLVMAVNDGGVPPILAQKHDSQRSAF
                     TSENLLREACRQKRITDSSIPNICVLDPDGNIVRSLLAGSEAMIAIAADRLRSRLP"
     gene            647..1792
                     /locus_tag="VM910_00640"
     CDS             647..1792
                     /locus_tag="VM910_00640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI61092.1"
                     /translation="MNHETAVSNAKEIADRILAPAARQNDKEARFSSEAVAALGPAGL
                     LGIVVPAEMGGSALGPRTLAAVIATLAEADASVAMVYLMHVCATATIAAARPGAAVAQ
                     TLNDIAAGKHLTTLAFSEAGSRSHFWAPISRAKRNGAGVRLTAKKSWVTSAGHAQSYV
                     VSALAPEGKSPTDSTLYLCASGTPGLSVAGPWDGLGMRANASAPMILEDCEIASDLQL
                     TDDGAGFKAMLEIPLPLFNLGSSAVALGLCRAAVAGTAAHLNGARFEHLGQTLGESLP
                     TLRAQLATMQIDTDGLAARIDDVADHLERPRDTTMLRVLESKAAAGDVAIAVTSAAMR
                     VCGGAAFSKHLAIERLFRDAHAGAVMAPTGDVLRDFVGKAILGLPLF"
     gene            1949..>2449
                     /locus_tag="VM910_00645"
     CDS             1949..>2449
                     /locus_tag="VM910_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HVI61093.1"
                     /translation="MVGIWEGMRRYFHEEAGLPVEVVLFQSYEAQVAALLALPGEPLP
                     RIDIGWNTNLAYIQADAWSDHRCRPIAMRDTDVGWMTKIVAVTGGPVAKLADVRNRTL
                     ALGSRDSGHAAILPVYFLEREGLAEGKDYRTLRFDTDLGKHGDTGTSEADVVRAVLDG
                     RGDAGAI"
BASE COUNT          440 a          759 c          806 g          444 t
ORIGIN      
        1 gcaaccgcgc caatggccgt cgcaagccgc gtctttgcgc ggccgggctg tggcgccttg
       61 cagatgatgc cgatcgccgc gctgctcacg gtagccgtga ccgcaggcga tcggctgcga
      121 tcgcaatcat ggcctcgcta ccggcgagca agctgcgcac gatgttgccg tcggggtcga
      181 gcacgcagat gttcggaata ctggagtccg tgatgcgctt ttgtctgcat gcttcccgca
      241 gcaaattttc ggacgtaaat gcggaccgtt ggctgtcatg cttttgcgcg aggataggcg
      301 ggacaccgcc gtcattgacc gccatcacca gacaaacctc gttacgccgt ggccgctcat
      361 tggtagcgtt ctcctcgaca gcgggcaagc atcgcccgcc cgcagcggtt cggaagagga
      421 aagccggcgc ccgcgtcaca aaaaaataga tctcgagtga cgtaacaaaa cagctccaag
      481 cgcgtaatga ccaccgcccg taaccgccct tgatatggca agagtttgtc atgccggaat
      541 cccatcgtgt tgctcccaag gtgcgggtcg cgtagcatcg ggggcgaaag agagcgggtt
      601 cgaattttcg aacgtggtag gccgcgccca gatgtcgagg gtcgtcatga atcacgagac
      661 cgcagtttcg aatgccaagg aaatcgccga tcgcattctc gcaccggcgg cgaggcaaaa
      721 cgacaaggaa gctcgatttt cgtccgaggc agtcgcggcg ctcggtccag ccggattgct
      781 ggggatcgtg gtgcccgccg aaatgggcgg ctcggcgctc ggaccgcgga cgctggctgc
      841 cgtcatcgcg acgctggcag aggccgacgc gtcggtcgcg atggtctatt tgatgcacgt
      901 gtgcgccacc gcaaccatcg cggcggcccg tccgggcgcg gcggtcgcgc agacgctgaa
      961 cgacattgcg gcgggaaaac atttgaccac tctggcattc agcgaagccg gatcgcgcag
     1021 tcacttctgg gcgcccatct cgcgcgcgaa gcgcaacggc gccggcgtgc gcctcaccgc
     1081 caagaagtcg tgggtgacga gcgccggtca cgcgcaaagc tatgtggttt cggcactggc
     1141 ccctgagggg aaaagtccga cggattcgac actttacctt tgcgccagcg gtacgcccgg
     1201 actgtccgtc gcaggcccct gggatggtct tgggatgcgc gccaacgctt ccgcgccgat
     1261 gatcctcgaa gactgtgaaa tcgcgtcgga tcttcagttg accgacgatg gcgccgggtt
     1321 caaggcgatg ctggagatcc cgcttccgct gttcaacctc ggatcgtcag cggttgcgct
     1381 tggtctctgc cgggccgccg tggccgggac cgcggcgcat ctcaacggcg cccgcttcga
     1441 gcatttgggc cagaccctcg gagagagcct gccgacgctt cgtgcgcaac ttgcgacgat
     1501 gcagatcgac actgacggcc tagcggcgcg catcgacgat gtcgcagatc acctcgagcg
     1561 cccccgggat accaccatgt tgcgcgtgct cgaaagcaag gcggcagcag gtgatgttgc
     1621 catcgccgtc acctcggcgg cgatgcgggt ttgcggaggt gcggcgtttt ccaagcatct
     1681 ggcaatcgag cggctgttcc gcgatgccca tgcgggcgcc gtcatggcac cgacgggcga
     1741 cgttttgcgc gacttcgtcg gcaaagccat tttgggacta ccgctgttct agcgatccga
     1801 tcctgacatt cgcgtcccat tccgggagcc gcccgcgagt gtcgggatca aaggaccact
     1861 ggcaagtggt gctgcggttg caagcgtgac aggtgaggcc atcatgaacc ggacgatctg
     1921 ggtaggtgcg gtcgcttatg atccgaaggt ggtcggcatc tgggagggca tgcggcggta
     1981 ctttcacgaa gaagcgggcc tgcctgtcga ggtggtgctg tttcagagct acgaggcgca
     2041 ggtcgccgcc ctcctcgcct tgcccggcga gccgctgccg cgcatcgata tcggatggaa
     2101 tacgaacctc gcttatatcc aggccgatgc ctggagtgac caccgctgcc gaccgatcgc
     2161 gatgcgcgat acggatgtcg gctggatgac caagatcgtc gcagtcaccg gcgggcccgt
     2221 cgccaagctt gccgatgtca ggaaccgcac tctggctctc ggcagccgcg acagcggcca
     2281 cgcggcaatt ctcccggtct attttctgga gcgcgaaggg ctcgctgaag ggaaagacta
     2341 ccgaacgctg cgctttgaca ccgaccttgg caagcacggt gacaccggca caagcgaggc
     2401 cgatgtggtc cgcgcggtgc tcgacggccg cggcgatgct ggcgcaatc
//
LOCUS       DATLJT010000032         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1379860,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000032 DATLJT010000000
VERSION     DATLJT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379860"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VM910_00650"
     CDS             complement(<1..638)
                     /locus_tag="VM910_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61094.1"
                     /translation="MPSQIESRTHDPVAATASASAQALLSMRGITKSYGDHVVLNHID
                     LDVASREKVAVIGPSGSGKTTILRAAIGLVKPDEGTIRIDGEYLWHVKATDGLRPAGE
                     RHARRVRRAVGIVFQQFNLFPHMTALENVREPLVHALGMRRQESDARATDLLCQVGLK
                     DHLGKRPAQLSGGQQQRVAIARSLALRPKIMLFDEVTSALDPELVGEVLRVVR"
     gene            complement(622..1287)
                     /gene="ehuD"
                     /locus_tag="VM910_00655"
     CDS             complement(622..1287)
                     /gene="ehuD"
                     /locus_tag="VM910_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HVI61095.1"
                     /translation="MSSFHWDWGFAWAILPELLQGLVVTVLATLLGSMVAFALGMVWT
                     LVRMLRIPLLTPSVSFFVEFIRGTPLLIQLYFLYYVLPTWGLTISPLPTGIFGLGLFY
                     SAYAAEIFRAGIEDLPPGQWEAALTLGLPVRRVWLGIILPQAIRSVVPILGNVVISMF
                     KQSSLLSTITMFELLAAGLNIGSVNYRYVEPLTLVGAFYFAISFLSARIVRSLEAKHA
                     FAD"
     gene            complement(1284..2009)
                     /gene="ehuC"
                     /locus_tag="VM910_00660"
     CDS             complement(1284..2009)
                     /gene="ehuC"
                     /locus_tag="VM910_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="HVI61096.1"
                     /translation="MATPQSLWDYLPYLATGIPVTLEITCIAMALAIVVGVILALGRN
                     SQIALLRWAAGFVIEFFRGSSALVQLFWAFYVLPFFGIQLPPLIAGILVLGLNEGSYF
                     SEVVRAGLDAVPKGQREAAVTLGLPFHYRLYRIVLPQALPIMVPPFGNELITMLKFTA
                     LVSLVTVQDLSSRASLISSTLGESGPIFGLTIIIYFVLALLLGGGVRLLERPVSRWAG
                     RDFSAAPAARRSPIPDWAVGGSR"
     gene            complement(2154..3095)
                     /locus_tag="VM910_00665"
     CDS             complement(2154..3095)
                     /locus_tag="VM910_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI61097.1"
                     /translation="MNRLHTQSFANGITVHASERDGSVNVRPMGRRSVLTGLAAASLA
                     CRIGNVVAQDQSGSGQGLLARLQQAKKVRVGVANQPPFSAVGPDGTLTGVAPSVSKAV
                     MKRLGILEFEPSIGTYGELIPGMLAGRWDFVTAALTVTKPRCEQVLFSDPVTLDARAI
                     VSFKGELADPPTRIADLVAKRTVVGIIAGGADLRLMLSVGVDQSNLKQFPQDSALMDG
                     LIAKRVQVAFGSHAALVRISQLRKLDLAITYPIEDDRPTFTACAFRKEDTDLYGAFQK
                     ELGALKASGEFQVIVEQNGFEAPPALVRATPVQACAF"
     gene            complement(3040..>3273)
                     /locus_tag="VM910_00670"
     CDS             complement(3040..>3273)
                     /locus_tag="VM910_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HVI61098.1"
                     /translation="VNTGNLMVRWTNDEYLSTKHRVINNYGVERYSIPVFFGPSADAV
                     IEVLPTCQSPQRPALYEPITYAKLREWYYGPRI"
BASE COUNT          731 a          995 c          980 g          567 t
ORIGIN      
        1 cgcacgacgc gcaggacctc gccgaccagc tccgggtcga gagcggaggt gacttcgtcg
       61 aacaacatga tctttggcct gagggcaagc gaccgcgcaa tggcgacgcg ttgctgttga
      121 cccccggaca gctgtgccgg acgcttgccg agatgatcct tgagaccaac ttggcacaag
      181 agatcggtgg ctcttgcatc gctctcctgt cgccgcatcc ctagcgcgtg aacgagcggc
      241 tcgcggacat tctccagcgc cgtcatgtgc ggaaaaagat tgaattgctg aaacactatg
      301 ccaaccgcgc ggcggaccct gcgggcatgc cgctcgccgg ccggtcgcag accgtcggtc
      361 gccttgacat gccaaagata ctcaccatcg atccggatgg tgccttcgtc cggctttacg
      421 agacctatgg ccgcgcgcag aatcgtggtt ttgccggatc cgctcggccc gataaccgcg
      481 actttctccc gcgacgccac gtcgagatcg atgtggttga ggaccacgtg atcgccatag
      541 ctctttgtga tgcctcgcat cgagagcagc gcctgcgcgg acgcactcgc cgtcgccgcc
      601 acaggatcgt gggtgcgcga ctcaatctgc gaaggcatgc ttcgcctcca aggagcggac
      661 gatccgagct gacagaaagc tgattgcgaa ataaaacgcg ccgacaagag tcaacggctc
      721 gacatatcgg tagttgacgg aaccaatatt cagtcctgcg gcgagcagct cgaacatcgt
      781 gattgtcgaa agaagcgagc tctgcttgaa catgctgatg acgacattgc ccagaatcgg
      841 cacgaccgat ctgatggcct gcggcaggat gatgccaagc cagacgcgac ggaccggtaa
      901 gccgagagtg agcgccgcct cccattgacc ggggggaagg tcctcgatgc cggcgcgaaa
      961 gatttcggct gcataggcac tataaaacag cccgagcccg aaaatgcccg tgggcaaagg
     1021 ggaaatcgtc aggccccatg tcggcagcac gtagtacagg aagtaaagct ggatcaacag
     1081 cggggtgccg cgaatgaatt cgacgaaaaa tgaaacggac ggtgtcaaga gggggatgcg
     1141 cagcatgcga acaagggtcc agaccattcc caacgcaaaa gcaaccatcg agccgagcaa
     1201 ggtcgccagc acggtgacga cgagcccctg cagaagctct ggcaggattg cccaggcgaa
     1261 gccccagtcc caatggaatg agctcacctc gagcccccca ccgcccaatc gggaatcggg
     1321 gagcgccgcg ccgcgggagc cgcggagaaa tcacggcctg cccagcggct gaccgggcgc
     1381 tcaagcagcc ggaccccgcc gcccagcaga agcgccagaa cgaaataaat gatgatggtc
     1441 aggccgaata tcggaccgct ttcgccaagc gtgctgctga tgaggctcgc gcgagacgac
     1501 aggtcctgga ccgtcaccag cgaaaccagc gcggtaaatt tcaacatggt gatcagttcg
     1561 ttcccgaacg gcggcaccat gatcggaagc gcctgcggca ggactattcg atacaggcgg
     1621 tagtgaaacg ggaggcccaa tgtaaccgcg gcttcgcgct ggcctttcgg aaccgcatca
     1681 agaccggctc ggaccacctc ggaaaagtag ctcccctcgt tcaatccgag gacgagaatc
     1741 ccggcgatca aaggaggaag ctggatgcca aaaaaaggca gcacgtagaa tgcccagaac
     1801 aactggacga gcgccgagct cccgcggaag aactcgatga cgaaacccgc cgcccaacgt
     1861 agaagcgcga tctgcgaatt ccggccaagc gccaggatta ctccgacgac aatggcaagc
     1921 gccatcgcaa tgcatgtaat ttccaacgtg accggtatgc cggttgcgag gtacggcaga
     1981 tagtcccaga gcgactgcgg tgtggccatg gcgaacgctc cgcgttgcgt ctcagcgagg
     2041 ttgcggaccg tctgcaacat tcgtgagcgg cgcttgcgtt tccgcaagca tctgacctgg
     2101 gggagtaaat acgagcggcc gaaacgctgg caagtccaat gccgctgctg cttctagaaa
     2161 gcgcaggctt ggacgggtgt cgctcgcacc aaagctggtg gagcttcaaa accgttctgc
     2221 tcgacgataa cctggaattc gccggacgct ttgagcgccc ccagctcttt ttggaaagcg
     2281 ccgtagaggt cggtatcctc tttcctgaac gcgcaagctg taaacgtcgg cctgtcgtct
     2341 tctatcggat aggtgattgc cagatcgagc ttccgcagtt ggcttattct gactaacgcc
     2401 gcgtgcgaac cgaatgcgac ttgcacccgc ttcgcgatca agccgtccat aagcgcggaa
     2461 tcttgcggaa actgcttcaa attcgattgg tccacaccca cggaaagcat caaccgcaaa
     2521 tccgcgccgc cagcaatgat tccgacgact gtccttttcg cgaccagatc ggcgatccgc
     2581 gttggcggat cagcaagctc gcctttgaac gagacaatgg cgcgggcatc gagtgtcacc
     2641 ggatcggaga ataagacctg ctcgcagcgc ggcttggtga cggtgagagc cgccgtaacg
     2701 aaatcccatc gtcccgccag catgccgggg atgagctcgc cataggttcc tatggacggc
     2761 tcaaattcaa ggatgcccag gcgcttcatg actgcctttg agacggacgg cgcgacgccc
     2821 gtgagcgtcc cgtcgggacc gacggcgctg aacggcggct gattggcaac cccgaccctt
     2881 accttcttag cctgctgcaa ccgggccaga agtccctggc cgctgccgga ttgatcttga
     2941 gcgacgacgt tccctatccg acacgcgagg ctcgccgcgg ccaggccggt caaaaccgaa
     3001 cgtcgcccca ttggcctcac attcacgctc ccgtcgcgct cagatgcgtg gaccgtaata
     3061 ccattcgcga agctttgcgt atgtaatcgg ttcataaagc gcgggccgtt gcggactttg
     3121 gcacgtcggc agcacctcga tgaccgcgtc cgcgctcggc ccaaaaaaca ccggaatgga
     3181 gtagcgctcg acgccgtagt tgttgatcac gcggtgcttg gtcgaaagat attcgtcgtt
     3241 ggtccagcgg accatgagat tgccggtgtt cac
//
LOCUS       DATLJT010000033         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1362,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000033 DATLJT010000000
VERSION     DATLJT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VM910_00675"
     CDS             complement(<1..804)
                     /locus_tag="VM910_00675"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI61099.1"
                     /translation="MGRLDGKVAVITGATSGIGLCTAEVFVAEGAKVVIAGRRVAEGE
                     KLAKKLGSACVFRQTDVTIEEQMRALIALSVERFGRIDCLFNNAGGPAQTGGIEGLDA
                     ARFDTAMATLVRSVMLGMKHAAPYMKKQGFGSIINNGSIAGRLAGFSSSIVYSAAKAA
                     VIHLTKCVAMELGEAGVRVNSISPGAIATGIFAKALGLSVEAAEDTPSVMREVSKTAQ
                     PIPRAGLPDDIAQAAVFLASDESSFVNGHDLVVDGAITGGRNWTQQQQGY"
     gene            1001..1975
                     /locus_tag="VM910_00680"
     CDS             1001..1975
                     /locus_tag="VM910_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HVI61100.1"
                     /translation="MKLTESKLIDEFRRGWQGVSPPSVTFSLAFAAGCLALSTLARWG
                     LAQIRPDVFFTPYIPAVLFATAVGGLRLGILTALASGLLGVAVNFGDTTADTSRIILL
                     VIFWAVCGLTVWGLEHYRAVAAQHRENARRLIQEEEYRKLVVNELQHRLKNKLSIIHA
                     VLHQVLESQPQVWSAIDHRLRALSATDDLIARVDGVGCDIRDLLISELGPYGHVRFNL
                     NGDRLFLPAKLAVSLALIFHELATNAGKYGAFSSARGLLQVSWTLSDDRLNVTWDETE
                     GPTIEQVGPPGFGTRLLKSALTPFDGKTEITFLKSGVHCTMQCRVPRS"
     gene            2124..>2363
                     /locus_tag="VM910_00685"
     CDS             2124..>2363
                     /locus_tag="VM910_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVI61101.1"
                     /translation="MRRQIVNELRISATEGDGPCAVEAELNALKDALMLLPAGVTVQD
                     EDGNLLLVNDVAAAQLGVSQSEAAEPSPQLIQRRSQ"
BASE COUNT          422 a          776 c          711 g          454 t
ORIGIN      
        1 gtaaccctgt tgctgctggg tccagttgcg gccgccggtg atcgcgccat cgaccacgag
       61 atcatggccg ttgacgaacg aagattcgtc gctggccagg aacactgcgg cctgagcgat
      121 gtcgtcggga aggcccgcgc gtgggattgg ctgtgcagtc ttgctgacct cacgcatcac
      181 agacggcgtg tcctccgcgg cctccacgga aaggccgagt gccttggcga aaattccagt
      241 ggcaatcgcg cccggagaga tcgagttgac gcggacgccg gcttcgccga gttccatcgc
      301 cacgcacttg gtgaggtgga tgacggccgc tttcgccgcg ctgtagacga tcgaggacga
      361 gaagccggcc agccgtccgg cgatgctgcc attgttgatg atgctgccga agccctgttt
      421 cttcatatag ggcgcggcgt gcttcatgcc gagcatgacg ctgcgcacca gcgttgccat
      481 cgccgtatcg aaccgtgcgg cgtcgagccc ctcgatgccg ccggtctgag ccgggccgcc
      541 cgcattgttg aacaggcaat cgatccggcc gaatctttcc accgaaagcg cgatcagcgc
      601 gcgcatctgc tcctcgatcg tgacgtcggt ctggcggaac acgcaggctg aacccagctt
      661 tttcgccagc ttctcgccct ccgcaacgcg ccgtcccgca atgacgacct tcgcgccctc
      721 ggccacaaat acctcggccg tgcacaagcc gatgccggag gttgcacccg tgatcactgc
      781 gaccttgccg tcgagccgtc ccatgccgtg ttcctcttcg tcactgtcaa atgccaattt
      841 tcgcttgcca aacaaatgct tccgaaccga caaaaggccg ccccgaccgg aaccggaaac
      901 cgcaaccaat atttgcgatc aacgcacgac aaggcaagct atgcgtgtaa tgggcagccg
      961 cgtattttcc tcaaggctgg tcgggccgtg ggtctggatg atgaaattga ccgaatcgaa
     1021 attgatagac gagttccggc gcggctggca gggcgtctcc ccgccgtccg taacctttag
     1081 cctcgccttt gcggccggct gccttgcgct ttcgacgctg gcgcgctggg gtctcgccca
     1141 gatcagaccc gacgtgtttt tcacgcccta tattccggcc gtgttattcg ccaccgcggt
     1201 tggcggtctg cggctcggca ttctcacggc cctcgcaagc ggattgctcg gcgtcgccgt
     1261 caatttcggc gacaccactg ccgacacctc gcgcatcatc ctgcttgtga tcttctgggc
     1321 cgtgtgcggg ctgactgtct ggggcctgga acattatcgc gcggtcgccg ctcagcatcg
     1381 cgagaatgcc aggcgcctga tccaggagga ggaataccgc aagctggtcg tcaacgagct
     1441 gcagcatcgg ctgaagaaca agctttcgat catccacgcc gtgctgcacc aggtgctgga
     1501 aagccagccg caggtctggt cggccatcga ccatcggctg cgcgcgctgt cggcgaccga
     1561 cgatctgatc gcccgcgtcg acggtgtggg ctgcgatatc agggatctgc tgatctccga
     1621 actcggccct tatggccatg tccgcttcaa cctgaacggc gatcgtctgt tcctccccgc
     1681 caagctcgcg gtgtcacttg cgctgatctt tcacgagctt gccaccaatg ccggaaaata
     1741 cggcgcgttc tcctccgcgc gcggcctgtt gcaggtgtcc tggacgctgt cggacgaccg
     1801 gctcaacgtg acttgggacg agaccgaagg cccgacgatc gagcaagtcg ggccgcccgg
     1861 cttcggcacc cgcctcttga aatcggcgct gacgccgttc gacggcaaga ccgagatcac
     1921 cttcttgaaa agcggcgttc attgcaccat gcaatgccgt gtgccccgaa gctgaacgcg
     1981 tctcggcctg cgggctgccg ttgacactct gttaatgaca actcgcggcg aaccggtcgg
     2041 catcggcccg gccgcatgcc gcgcccgtaa ttctacgagc catttaacca aacctaagag
     2101 ggaactgccc acaatccgcg gccatgcggc gtcaaatagt caacgaattg cggatttccg
     2161 ccacggaggg cgacggcccc tgcgcggtcg aggcagaact gaacgctctg aaggacgcgc
     2221 ttatgctcct gccggccggt gtcacggtgc aggacgaaga tggcaacctg ttgctggtga
     2281 acgatgtcgc ggcggcccag ctcggggtgt cccaaagcga ggctgccgag ccttcgccgc
     2341 aactgatcca gcgccgcagc caa
//
LOCUS       DATLJT010000034         3972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2069348,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000034 DATLJT010000000
VERSION     DATLJT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069348"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VM910_00690"
     CDS             complement(<1..475)
                     /locus_tag="VM910_00690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI61102.1"
                     /translation="MFHVVNRTTENILGTGVVQLTSGGDKSLTQAEVGGLMVHPAARG
                     FGIASLLVKVMMLYAIKQSGRDAPDEQYLAHVVDGNGGPLHALLEAGFRPAGHVDIHR
                     GDIDAVIDHMLKDGESEVRLQAFVFDRQAVGKLVLSLWTFVREHRGLITRSDGAGD"
     gene            complement(1011..2468)
                     /locus_tag="VM910_00695"
     CDS             complement(1011..2468)
                     /locus_tag="VM910_00695"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61103.1"
                     /translation="MKRREFIALLGGAAAWPLAARAQQPKIATVGILVAGNTSPEAFL
                     QGFRAALAAAGLIEGQNVQLEVRSAEGSDTLLPQKATELVRLKADVIVAFLTPAVQAA
                     RHVTSDIPIVMAPAGDPVATGLISSLARPGGNITESNQAAAALKLMERWIEQGQHLAA
                     IARHLRLTKGASADLMRKAGQKALDAKDVIAVIEGIAAIVANKAFDLVDPVVMPGLRL
                     LTEQNDTRWVSAIWFMPELGEFLTHLSESQSEIVLENLVLCARIDHDEEHILTAIAGS
                     YPRSVWHFFKARLDRAATKERDEEHYEDIPFQLHELVQPLSRDPSFAVDAVRSWYAAD
                     QSLFQYRGGKLLHNVFPGFSSALGAKLVAIVQECTDAAINFVLTILQTYQGQPALHDV
                     CKEVVDKLPENDSRLNEIEVILESTGIISGQFGFVEAYQRKKNEVSPWLSDPRPKVRA
                     FAERYQRTLDRSIAAEQRRSETDYELRRREWPEEE"
     gene            complement(2465..2899)
                     /locus_tag="VM910_00700"
     CDS             complement(2465..2899)
                     /locus_tag="VM910_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61104.1"
                     /translation="MQAAGRALGRQILVLNASTEGEIDAAFAALVQLRAGALAVGGDA
                     FLNSRREQLVALAARHGVPAVYPWPEYVTAGGLMSYGASLIGAYRQAGVYDGRVLKGA
                     KPANLPVQQPTKFELMINLKTAKALGLNVPDRLLALADEVIE"
     gene            complement(3106..3750)
                     /locus_tag="VM910_00705"
     CDS             complement(3106..3750)
                     /locus_tag="VM910_00705"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI61105.1"
                     /translation="MSFARGILFVLTGLVLAGYMQSGPVFDANFTPRDRQQLAHPPYQ
                     QAWIPPAYQRQIVDYHRKEAPGTIVVDTDARYVYYVLRQGKAVRYGAIVGEQGQAWSG
                     VATVDRKEEWPGWTPTAGEKRRLGPLPNYVEGGAANPMGARALYLYANGQDTLYRIHG
                     TNQPGWIGHAISSGCIRLTNEDIIGLYSRVKTGATVVVLGPGQGSNRVVSASAL"
BASE COUNT          755 a         1224 c         1173 g          820 t
ORIGIN      
        1 tatcacctgc cccgtctgat cgagtgatca ggccgcggtg ctcgcggacg aacgtccaaa
       61 gggacaaaac cagctttccc accgcttgac ggtcgaagac gaaggcctgc agacgaactt
      121 cggactcacc gtccttgagc atgtggtcga tcaccgcatc gatgtcgccg cggtggatgt
      181 cgacatgtcc ggccggtctg aagccggcct cgagcagcgc gtggaggggg ccgccattac
      241 catcgacgac gtgggcgaga tactgctcat ccggcgcatc gcgcccgctt tgcttgatgg
      301 cgtagagcat catgaccttc acgagaagag acgcgatacc gaagccgcgt gcagccgggt
      361 gaaccatcag gccaccgacc tcggcctgcg tgagcgattt gtctccgccc gatgtcagtt
      421 gaacgactcc ggtgccgaga atgttttcag tggtccggtt cacgacgtga aacaagcggt
      481 gctgccgggc tgcagcgcgg aaaacatgcg ccggacgatc gaaaagtgtt ccctcgttca
      541 gcgcctcaat gaaggtgaag ggaagccggt attcggcctc cctcgtcagt cggatctcga
      601 ccctgtcctg gtccctgatc attgtgggtc gatggaccat ctggcggcgc ctcgcttgcg
      661 gggtgatgag cggtgagcgt cggtgcgttc cagcgggcta tgacgtccgc ttcgaggcgc
      721 aaaggactct gccccgcaaa aaaacgcggc gtgacgaacc gcgctgaggg ggcatgacac
      781 gttagtaact gttacggtca gcaacgccat ctttaaaccc attcgccgcg aacgagacca
      841 ttcgtcttac gcggtccgtc tccgccaaat gtgagtgaaa ttgcttgata attttaatgt
      901 gacttgtgtt tgaccatcac cttgcccatg ccggaacgtc cgatggccgc tgttcctggc
      961 ccttcgcttg cgttggaccg cgcgcattgt gatgccgcgc accacggcct ctattcttct
     1021 tcaggccatt ctcttcgccg cagttcataa tcagtctccg agcggcgctg ttccgccgca
     1081 atgctacgat cgagtgttcg ttggtatcgt tctgcgaaag cgcggacttt cgggcgcgga
     1141 tcgctgagcc atggagaaac ttcgttcttt tttcgttgat acgcttcgac gaagccaaac
     1201 tgtccggata tgataccggt gctttccaag atgacctcga tctcattcag acggctatcg
     1261 ttctccggaa gcttgtcgac gacctctttg catacgtcgt gcagagcggg ttgcccttgg
     1321 taggtttgta ggatcgtcaa gacgaaattg atcgcggcgt ctgtacattc ctgaactatg
     1381 gcaacgagct tcgcccccaa cgcggagctg aaccccggaa agacgttgtg tagcaacttg
     1441 ccgccgcggt actgaaacag gctctgatcc gcagcatacc aactgcggac cgcatcgacg
     1501 gcgaacgacg ggtcccggga caagggctgc accagttcgt gcagctggaa cgggatgtct
     1561 tcataatgtt cttcgtcgcg ttcctttgtc gcagcgcgat ccaaacgagc cttgaagaag
     1621 tgccagacgg agcgcggata gctgccggcg atcgccgtca agatgtgctc ttcgtcgtgg
     1681 tcgatgcgag cgcacagcac gaggttctcc aatacgatct cgctttgaga ttctgatagg
     1741 tgtgttagga actcacccag ctccggcatg aaccagatgg cgcttaccca ccgcgtgtcg
     1801 ttctgttctg tgagcagccg taacccgggc atgacgaccg ggtctaccaa atcgaacgct
     1861 ttgttcgcga caatcgcggc gatgccttca atgacagcga tcacgtcctt tgcgtcgagg
     1921 gctttctgcc cggctttgcg catgaggtct gcggaggcac ccttggtgag ccgcaggtgc
     1981 ctcgcaatgg ccgcgaggtg ctgaccttgc tcaatccaac gctccataag cttgagtgct
     2041 gctgccgcct ggttgctctc ggtgatgttt ccacccggcc gggcgaggct ggagatgagg
     2101 ccggtcgcga ccggatcgcc ggccggcgcc atcacgattg gaatgtcgct cgtcacatgc
     2161 cgcgccgcct ggacggccgg tgtcaggaag gcgacgatca cgtctgcctt gaggcgaacc
     2221 agctctgtgg ccttctgcgg cagaagggtg tcgctgcctt cggccgagcg cacttccaac
     2281 tggacgttct ggccctcgat caggccggcc gctgcaagcg ccgcacgaaa accctgcaag
     2341 aacgcttcgg gactcgtgtt gccagcgacg aggatgccaa cggtcgcgat cttcggctgc
     2401 tgcgcgcgcg ccgcgagcgg ccacgcagcc gcgccgccga gcagcgcaat gaactcgcgt
     2461 cgcttcattc gatcacctcg tcggcgagcg cgagcagtcg atccggcaca ttcagcccaa
     2521 gcgctttcgc cgttttgagg ttgatcatca gttcgaactt ggtcggttgc tgcacgggaa
     2581 gattagcggg cttagcacct ttcagaaccc ggccgtcata aacgccggcc tgacggtacg
     2641 cgccgattag gctcgctccg tagctcatca ggccgcccgc cgtgacgtat tcaggccatg
     2701 ggtaaaccgc cggcaccccg tgccgtgccg ccagcgcaac gagttgttcg cggcggctgt
     2761 tcaagaacgc atcgccaccc acagcgagcg cgcctgcccg cagctggacc aaagccgcaa
     2821 atgctgcatc gatctcgcct tcagtgctgg cattcaggac aaggatttgc cgcccgaggg
     2881 cgcgccccgc cgcctgcatg gctttcgtag tggaccttca ccagcacggc gatcaagctg
     2941 gagttagcta tcagctcgtg cagcaattca atcctgatca ctatcgcgtg ccggtgctcg
     3001 tcagtgaacg cgcaaggtcg cccgatgaat acccgtgcgg tccctgtgtg ccgtgggagt
     3061 ggccgctgca cacgagccga cgatcgcctc agggccgtcg tggtcttata gagcgctggc
     3121 cgagaccact cggttcgacc cctgccccgg tcccagcacc accacagtcg cgccggtctt
     3181 cactcgactg tacagaccaa tgatgtcctc attggtcagt cggatgcagc ccgacgaaat
     3241 cgcatgtccg atccatccag gctggttcgt gccatggatg cgatacagcg tgtcctgccc
     3301 gttggcataa aggtagagcg cgcgggcgcc cattggatta gctgcaccgc cttcgacata
     3361 gttcggaaga ggtcctagcc gccgcttctc acccgcggtt ggcgtccatc ccggccactc
     3421 ttccttgcgg tcgaccgtcg ccacgccgga ccaggcttgg ccctgctcgc ccacgatcgc
     3481 gccatagcgg acagccttgc cttggcgaag cacgtagtag acgtagcgtg cgtcggtatc
     3541 gaccacgata gttcctggcg cttctttccg gtggtaatcg acaatctgac gctgataggc
     3601 gggtggaatc cacgcctgct gataaggcgg atgcgccagt tgctgtctgt cgcgaggcgt
     3661 gaaattggcg tcaaacactg gaccggactg catgtagcca gcaagcacca gtccagtcaa
     3721 aacaaacaag atgcctcgcg cgaatgacat cgctcccccg aagcccaaat cagggtgtta
     3781 aagtttacct taattgtgag agtccagtct gtgaccagtc ggtcacagcc catgcaaatg
     3841 tgggtcgcat cggccagtgt acgtcatgcg caacatccgt ttcagcaacc gtgcgagcga
     3901 acattctgtg gatctgaaat tcggaagagc ccagggcccc gcagcgtggc gccgtttcaa
     3961 aaaacttgac ag
//
LOCUS       DATLJT010000035         2194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1609584,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000035 DATLJT010000000
VERSION     DATLJT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609584"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..446)
                     /locus_tag="VM910_00710"
     CDS             complement(69..446)
                     /locus_tag="VM910_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HVI61106.1"
                     /translation="MTTMGRRYWAIAEGFIPGESTGPAPFMTSHETVCILNTGDTDAN
                     VEITIFFQDRGPVGPYRFVVPAQRTKHLLFNDLNDPAPVPRNTPYASTIVSDVPIVCQ
                     HTRLDSRQAENALMSTIAYAGTE"
     gene            complement(525..1265)
                     /locus_tag="VM910_00715"
     CDS             complement(525..1265)
                     /locus_tag="VM910_00715"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HVI61107.1"
                     /translation="MSREKRPFEAPFVTTPQMAEVDRAMIEDFKIELIQMMENAGRNL
                     AHLTRIRFLDGDPQGRRVIVLAGGGGNGGGALVAARRLHNWGASVDVILGQPSSAMSE
                     IPAHQLEIVERMDISVMAASAAANLAPATVILDGLIGYGLKGPPRGAVAALIRWANEQ
                     SAPTLALDVPSGVDTTNGTFFDPVIHAAATMTLALPKEGLRTPGVERLVGELYLADIS
                     VPPALYARPPLNMCVESLFARDEVIRLR"
     gene            complement(1281..>2194)
                     /locus_tag="VM910_00720"
     CDS             complement(1281..>2194)
                     /locus_tag="VM910_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVI61108.1"
                     /translation="ICVDPALQHPNVTLLTNAFVSRLQTDAAGRTVTTVHVERQGNAE
                     EYAAEIVVLSCGAINSALLLLRSANDKHPNGLANRSGVVGRYYMRHNNAAFLAISKQA
                     NPTVFQKTLALNDFYFGSNDWDYPLGHIQMLGKSHGEMLKGEAPDWLFWKPEMSFDVM
                     ARHSIDFWLTSEDLPDPENRVMLDSDGKVVLHLTENNMEGHRRLMAKLKEMLATLDCH
                     PHLLPRSLYLGKNIPIGGTAHQCGTIRFGRDPESSALDVNCKAHDLDNLYVLDGSFFV
                     SSGAVNPALTIMANALRVAQHLEQRLR"
BASE COUNT          430 a          661 c          680 g          423 t
ORIGIN      
        1 cgcgaacgcc gcaccgacgc gccgcaggca agctccgggc cgaatcagaa ttacgacgtg
       61 caggtccgtc attcggtgcc agcgtaggcg atcgttgaca tgagggcgtt ctcggcctgt
      121 ctcgaatcga gacgagtgtg ctggcaaacg attgggacgt cagagacgat tgtgctcgca
      181 tagggagtgt tgcgcggtac gggagcgggg tcattgaggt cgttgaaaag cagatgcttg
      241 gtccgttgcg ccggcactac gaagcggtat ggtccaaccg gaccgcgatc ctgaaagaag
      301 atagtgatct ccacgttcgc atcggtgtca ccggtgttca atatgcagac cgtttcatgg
      361 ctcgtcatga acggagccgg cccggtgctc tcgccgggaa tgaagccttc cgcgatcgcc
      421 caatacctcc gtcccatggt ggtcatcggc tgcttctcct gtgttcagcg cggctttgtg
      481 cacgctcctg cgctgccgag ccacgcagca taaatcccgc gggctcaacg cagccggatc
      541 acttcatccc gagcgaaaag cgattccaca cacatgttca ggggcgggcg agcatacaga
      601 gccggtggca cgctgatgtc ggcaaggtag agctcaccga ctagtcgctc gacgcctggg
      661 gtgcggagcc cttctttcgg cagagccagc gtcatggtcg cggcggcgtg gatcaccggg
      721 tcgaagaaag tcccgttggt agtgtccacg ccggagggca cgtcaagcgc cagagtcggc
      781 gcgctctgct cgtttgccca gcggataagc gcggccacgg caccgcgcgg cgggcccttc
      841 aggccatagc cgatcagccc atcgagaatg acggtcgctg gagcgagatt tgccgcggca
      901 gaggcagcca tgactgaaat gtccatgcgt tcaacgattt cgagctgatg tgccggaatt
      961 tcggacatgg ccgatgacgg ctggcccaga atcacatcga ccgacgcccc ccaattgtgc
     1021 aatcgtcgcg ccgcgaccag cgcgccgccg ccgttgccgc caccgcccgc cagcacgatg
     1081 acgcgccggc cctgagggtc gccgtcgaga aagcggatcc gcgtgagatg cgcgagattg
     1141 cgcccggcgt tctccatcat ctgaatcagc tcgatcttga aatcctcgat catcgcgcgg
     1201 tcgacttccg ccatctgagg cgtcgtgacg aagggcgctt cgaatggacg tttttcgcgg
     1261 ctcatgacgt cctcgacccg tcagcgcagt cgctgctcga ggtgctgggc tacccgcaga
     1321 gcattcgcca tgatcgtcag cgcgggattc acggccccac tcgagacaaa gaagctgccg
     1381 tcaagaacgt agagattatc gagatcgtgc gccttgcaat tgacgtcgag cgcggaactc
     1441 tcgggatcgc ggccgaatcg aatggtgccg cattggtgcg ctgtgcctcc aatcgggatg
     1501 tttttgccga gataaagcga tcgaggaagc agatgcggat ggcaatccag cgtcgcgagc
     1561 atctccttga gcttcgccat caagcgccga tgcccctcca tgttgttttc ggttagatgc
     1621 agcaccacct tgccgtcgga atcgagcatg acacggttct ccggatcggg cagatcctcg
     1681 gaggtcagcc aaaaatcgat cgaatgccgc gccatgacgt caaatgacat ctctggcttc
     1741 cagaacaacc aatccggcgc ctcgcccttg agcatctcgc cgtgggattt gccgagcatc
     1801 tggatatgac caagcgggta gtcccagtca ttcgagccga agtagaaatc attgagcgcg
     1861 agcgtttttt ggaacacggt gggattggcc tgcttcgaaa tcgcgaggaa ggctgcgttg
     1921 ttgtggcgca tgtagtaacg tccgaccact cccgagcggt tcgccaatcc gttcggatgc
     1981 ttgtcattgg ccgagcgcag cagcagcaag gccgagttga tcgcgccaca cgagagcacc
     2041 acgatttcgg cggcatactc ctccgcgttc ccctgccgct cgacgtgaac cgtcgtaacg
     2101 gtgcggccgg ccgcatcggt ctgcaaccgg ctcacgaatg cgttggtcaa aagcgtcaca
     2161 ttcggatgct ggagtgccgg gtcgacgcag atga
//
LOCUS       DATLJT010000036         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_690761,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000036 DATLJT010000000
VERSION     DATLJT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690761"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VM910_00725"
     CDS             complement(<1..305)
                     /locus_tag="VM910_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61109.1"
                     /translation="MELVEAIILSVLAASTPLLIAATGELVTERSGVLNLGVEGMMIV
                     GAACGFGGAWPSGSIFVGALCGIAAGTLMSLIFAVMTLGLAVNQVATGLALTILGVGL
                     "
     gene            complement(292..1383)
                     /locus_tag="VM910_00730"
     CDS             complement(292..1383)
                     /locus_tag="VM910_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61110.1"
                     /translation="MQLVLEKRAERSNTIALISPLIAIGLTIVTMVILFAMLGKNPLS
                     ALFVYFIDPLTDSYSLQEIAVKATPLVMIAIGLSLCYLANAWNIGAEGQFLMGAVAGS
                     WIAVKTQGTDAGYWVLPAMLLLGAAAGALYALIPAICKVRFGASEILTSLMLVYVADL
                     LLDYLVRGPWRDPGGFNFPTTAEFDPVATVPLLVPGGRLHLGAIIALVVVAAATVLLG
                     RTIKGFEIRVVGAAPRAARFGGFNANQLILLTFAVSGALAGLAGIIEVAGPVGHLQPG
                     VSPGYGFTAIIVAFLGRLNPIGILIAGLFLALTFIGGEQAQIAMKIPLDVTKVFQGIL
                     LFYVLACDSLILYRFRLISASRQVPSGTG"
     gene            complement(1367..>2500)
                     /locus_tag="VM910_00735"
     CDS             complement(1367..>2500)
                     /locus_tag="VM910_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61111.1"
                     /translation="WQLSVGERQRIEIVRALMQDPKFLILDEPTAVLTPQEADQLFIV
                     LDRLKAEGRAILYISHKLEEVKRLCDTATILRGGRKVATCNPKTETAASLARMMVGSE
                     IKEVKAAAARKTTVPRLVVNDLSLEPDDPHGVRLQHISLELKGGEILGIAGVAGNGQD
                     ELFAALSGERMARDPGTIVIEGIAAGHLSITERRRLGAAFVPEERLGHGTAPRMKLSE
                     NALLTGHAASDMVKRGFVNTAATLKTVDRATETFDVRKAKRDPEAASLSGGNLQKFVV
                     GREILRSPAVLVVSQPTWGVDAGAAAVIRQALLDLAAGGAAVLVTSQDLDELAEIADR
                     IAVIFHGRLSAPLLAAEATRENLGLLMGGSSLEHKEAAHAARA"
BASE COUNT          478 a          799 c          807 g          416 t
ORIGIN      
        1 agaccgacgc cgaggatcgt caaggcgaga ccggtggcaa cctggttgac cgcaagcccc
       61 agcgtcatca ccgcaaagat cagggacatc agggtcccgg cggcgatgcc gcaaagcgcg
      121 ccgacgaaga tcgaaccgga cggccacgcg ccgccgaagc cgcacgctgc gcccacgatc
      181 atcatgcctt caacaccgag gttgagaacg ccggatcgct cggtcacgag ctcaccggtg
      241 gctgcaatca gcagcggcgt cgatgccgcc agcaccgaca ggataatcgc ctcaaccagt
      301 tccactgggc acctgtcgtg acgcggaaat aagccggaag cggtaaagaa tgagcgagtc
      361 gcaggcgagc acgtagaaca gcaggatgcc ctgaaacacc ttggtgacgt ccaacgggat
      421 tttcattgcg atttgggcct gttcgccacc gatgaaagtg agggcgagga acagtcctgc
      481 aattaatatt ccaatcgggt tcagtcggcc gaggaaggcg acgatgatcg cggtgaagcc
      541 gtaacccggc gatacgccgg gctggagatg gccgaccggg ccggcaacct cgatgattcc
      601 ggccaacccc gcgagggccc cggatacggc gaacgtcaag aggatcaact ggttggcatt
      661 gaacccgccg aaccgcgcag cacggggagc ggcgccgacg accctgattt cgaaaccctt
      721 gatggtgcgg ccgagcagca cggtggcggc ggcgacgacc acgagcgcaa tgatggcgcc
      781 aaggtgcagg cggcctcccg gaacgagcaa tggtacggtc gcaacggggt cgaactcagc
      841 ggtggtcggg aagttgaagc cgcccggatc gcgccagggg ccgcgcacga gatagtcgag
      901 caagagatcg gcgacgtaga ccagcatcag gctcgtcaga atctcgctgg cgccgaacct
      961 gaccttgcag attgcaggga tcagcgcgta gagcgcgcct gcggcggcac cgagcaggag
     1021 cattgccggc aacacccaat agccggcgtc cgtgccttgc gtcttgaccg cgatccagct
     1081 ccccgcgacg gcgcccatca gaaattgtcc ctcggcgccg atgttccagg cgttggcgag
     1141 gtagcagagc gatagcccga tcgcgatcat cacaagcggt gtcgccttca ccgcgatctc
     1201 ttgcagcgaa tagctgtcgg tcaagggatc gatgaagtag acgaacagcg cggagagcgg
     1261 attcttgccg agcatggcaa acaggatgac catggtcacg atggtgagcc cgatcgcgat
     1321 caacggcgag atcagtgcga tcgtgttcga tcgctcggcg cgcttttcaa gcacgagctg
     1381 catgcgccgc ttccttgtgt tcaagactgc ttccgcccat cagcaggccg aggttttcgc
     1441 gcgtcgcttc ggcggccaga agcggcgccg acaggcgccc atgaaatatc accgcgatgc
     1501 ggtcggcgat ttcagccagc tcgtcgagat cctggcttgt caccagcacg gccgcgccgc
     1561 cggctgcgag gtcgagcagc gcctggcgga tcacggcggc ggcgccggca tcgacgcccc
     1621 aggtcggctg gctcaccacc aggaccgcgg ggcttctgag gatttcgcga cccaccacga
     1681 acttctgcag gttgccgccg gacaggcttg ccgcttcggg atcgcgcttg gccttgcgta
     1741 cgtcgaaggt ctctgtggca cggtcgactg tcttcagcgt agccgcagtg ttgacaaagc
     1801 cacgcttgac catgtcgctg gcggcatgtc cggtcagaag cgcgttttcc gaaagcttca
     1861 tgcgcggcgc ggtaccgtga ccgagccgtt cctcaggcac gaacgccgct cccaacctgc
     1921 ggcgctctgt gatcgaaagg tggcccgcag caattccttc gatcacgatg gtgccgggat
     1981 cccgggccat gcgctcgccg gacaaggccg caaagagctc gtcctggccg ttgccggcga
     2041 cgccggcgat cccgaggatc tcgccgccct tcagctcgag cgaaatgtgc tgcagcctca
     2101 cgccgtgcgg atcgtcgggc tcgaggctga gatcgttgac gaccagcctc ggcaccgtgg
     2161 tctttcgcgc tgccgccgct ttcacttcct tgatctcgga gccgaccatc atccgcgcga
     2221 gcgaggctgc ggtctccgtc ttcggattgc aggtcgctac cttcctgccg ccacgcagga
     2281 tggttgccgt gtcgcagagc cgtttcacct cctcgagctt gtggctgatg tagaggatcg
     2341 cgcgaccctc ggccttcagc cgatcgagca cgatgaatag ctggtccgct tcctgcggcg
     2401 tcagcacggc ggtcggctcg tcgaggatca gaaatttcgg atcctgcatc agcgcgcgga
     2461 cgatctcgat gcgctggcgc tcgccgaccg acagttgcca
//
LOCUS       DATLJT010000037         4024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1542,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000037 DATLJT010000000
VERSION     DATLJT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VM910_00740"
     CDS             complement(<1..183)
                     /locus_tag="VM910_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI61112.1"
                     /translation="MTTRRAACSCGQLQLTIEGEPSRISMCHCLECQRRTGAAISNQA
                     RFRREQVTFAGKSTAWM"
     gene            complement(451..1533)
                     /locus_tag="VM910_00745"
     CDS             complement(451..1533)
                     /locus_tag="VM910_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI61113.1"
                     /translation="MRINAKGLHWTPVTLADGTKKIYWYAWRGGPRLSGEYGSPEFIA
                     SYNAAVATKVVVPEGRLLSLMQAFQQSQDFLSLRERTRADYIKQIAKIEQKFGDAPLK
                     ALSDPRTRGVFLDWRDELALRSQRQADYVWQVLARVLSWAKDRGKITINPCERGGRVY
                     HGTRVDFVWSVDDEAAFLEHAPAHLHLPLLLALWTGQRQGDLLRLPWSGYDGSTIRLI
                     QSKSKRRDAVRPVRVLIPVGAPLKAALDAAPRRSPIILTSKAGRPWTSGGFRASWRIG
                     CDAAGIIGITFNDLRGTAVTRLALVGCSEPEIATITGHSLRDVRSILDAHYLHRDIEL
                     ARSAITKLEMGYAKSAGAKTGTDSSK"
     gene            complement(1535..1747)
                     /locus_tag="VM910_00750"
     CDS             complement(1535..1747)
                     /locus_tag="VM910_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61114.1"
                     /translation="MPKPRKFTPRARNRFRSVEVARAIRAARAAGGERVEIDPETGTI
                     SVVIGGKLGESVADNEVENWFSKQKG"
     gene            complement(2592..3023)
                     /locus_tag="VM910_00755"
     CDS             complement(2592..3023)
                     /locus_tag="VM910_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61115.1"
                     /translation="MNVNRLSKPHWWQERLRSAPTTKCVLAAVLLILLALSAPVHADD
                     PIAEWLKQDESDGQNDQVRPDLHALTDQQLQDIFNIGRDQADSHQLIERGSERHPRVL
                     YIWFVTIAAGFGAVIGAVKAVPFVRRQLRTLAVPVQMKDQS"
     gene            complement(3061..3414)
                     /locus_tag="VM910_00760"
     CDS             complement(3061..3414)
                     /locus_tag="VM910_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61116.1"
                     /translation="MFYIHADPPKSSPPIPVRVSRRSQLTTALHQHARPIVIEDQELA
                     GPFVRLLRARQLRLWGLGEFVANTMSSGIDQSYGANVEAHWYVGRYVLPGNLQKVILK
                     PKPYGAGRPPRHLQA"
     gene            complement(3543..3695)
                     /locus_tag="VM910_00765"
     CDS             complement(3543..3695)
                     /locus_tag="VM910_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61117.1"
                     /translation="MLVTPLAWISLMMGRTLAANVSAISLRPVREAHRLFEARAFRRL
                     DIGQAF"
BASE COUNT          744 a         1252 c         1221 g          807 t
ORIGIN      
        1 catccacgcc gtagacttac cggcgaaggt gacctgctcg cggcggaagc gcgcctggtt
       61 gctgatcgcg gcgccagtgc ggcgttggca ctccaggcag tggcacatgg agatgcgcga
      121 aggttcgccc tcaatcgtaa gctgaagctg cccgcaactg caggcggcgc gtcgggtggt
      181 catggactgg ctccgtggct gtcgatatgg cgcagtgtag gctggacttc gaaacgatgc
      241 accatgggca gcggaatcct gtgaccagct aatgtcggct tctggcagcc cccgaaagcg
      301 gacctcgagc gatacgacgg gcatgtccgc tgagcgccag gagcggactc atgttccgcg
      361 actagagctt cgactcctgg tcccgcagcc agctgatccc attgcgcttt ttccgttttc
      421 ctcgttacac tcgttcgacc attttgaggg tcattttgaa gaatccgttc ctgttttcgc
      481 cccggcggac ttcgcgtacc ccatctcaag cttggtgatt gcgctgcgcg ccaactcaat
      541 atcgcggtgc agataatgcg cgtcgaggat cgagcgcacg tcgcgcagtg agtgccccgt
      601 gatggtcgct atctcgggct cgctgcatcc aacgagcgca agccgcgtga ccgccgttcc
      661 acgcaagtcg ttaaacgtga ttccgatgat cccggctgcg tcacagccga tccgccaact
      721 ggcgcggaag ccgccagatg tccacggcct gcccgccttg ctggtgagaa tgatcggcga
      781 acgccttggt gccgcatcga gcgcggcctt gagcggcgcg ccgaccggga tcaagacgcg
      841 gacgggccgg acggcatcgc gtcgcttgct cttcgactga atcagacgga tcgtggaacc
      901 atcgtagccg gaccacggca ggcgcaagag atcgccctgc cgctggcccg tccacagcgc
      961 gagcaagagc ggcaagtgca gatgcgccgg agcgtgttcg agaaatgccg cctcgtcgtc
     1021 gacgctccac acgaaatcga cgcgcgtccc gtgatagacg cgcccaccgc gctcgcacgg
     1081 attgatcgtg atcttgccgc gatccttcgc ccacgagagc acccgcgcca aaacctgcca
     1141 cacgtaatcg gcttgtcgct gtgatcgcaa cgcaagctca tcgcgccagt cgaggaagac
     1201 tccgcgcgtg cgcggatcgg acaatgcctt gagcggggcg tcaccgaatt tctgctcgat
     1261 cttggcgatc tgcttgatgt aatcggcgcg cgtgcgctcg cgtagactaa gaaagtcttg
     1321 gctctgttgg aaggcttgca taagcgacag caaccggccc tcgggcacca ctaccttggt
     1381 cgcaacagcg gcgttgtagc tcgcaatgaa ttcgggcgag ccgtattcgc cggagagccg
     1441 cggaccgccg cgccaagcgt accagtaaat tttcttcgtg ccgtctgcca gcgtgaccgg
     1501 cgtccagtgc aggcccttag cgttgatgcg catcttaccc tttctgtttg ctgaaccagt
     1561 tctcgacttc gttatccgca acgctctcgc cgagcttgcc gccgatcacg acgctgatcg
     1621 tgccggtctc gggatcgatc tcgacgcgct cgccaccggc ggccctagcg gctcgaattg
     1681 cgcgagcaac ttcgacggac cgaaagcgat tgcgcgcgcg cggagtgaac ttcctgggct
     1741 taggcatgcg caaacatttt gcgcagcgcg cagccatttc gtcaacgaag caatcatctc
     1801 ggcatcaaaa aagtgaatgt gcgcgccgcc gtgcggagcg cgttttgctc cgtaaagcgg
     1861 tttttactgc gtaaagcggt ttttactgcc gtaaagcgct tttaaagcgc tttttgctgt
     1921 gtggagcgcg tttcaccgtg tgaccccgcg cgcgtcgccg tgcgattttt ttctgacggt
     1981 cgcgtcgatc gcgatctttc gcctgcttgc tccagcggcg gatgcgtcgc tggcgtcgtg
     2041 cctcgatctg ctggcactct tgaactcgcg ctcgcatgcc cgtagccgtt tgacgattcg
     2101 cgcatgatga gtgagagcgc gcgcggcaag atcgacctgg gctgagctgg tcgagctggg
     2161 cggcatgctg ggcaccgagg cgccaggcgc gccggtctca ccccatgaga tcatggtcaa
     2221 acagggcaga agcctcccgg tcgaatactg ggccgatccg ttcttaagta aagcgtcgaa
     2281 gttggccgcc cgcgtggctt gcctcatcag gttggcgagc gcggcgcgaa ccctggcccg
     2341 ctgccatcgc gcgtacgcgc tccatggccg cagaagcccg tcgtggccca agccgcggat
     2401 gacctaagtg tgcgtccacg gccaccggag agtcaggggg catcctacgc gctccattgc
     2461 cggggcgggc gctcagcagt cgcgctgaag ccgccgctcg gggttaggac gccttcgcag
     2521 ccggcaacgt gatctgagtt aactacgccg gggattgcat ttttggagcg ctcgtccgcc
     2581 gcggggtggc atcagctttg atccttcatt tgcactggga ccgctaaagt tctcagttga
     2641 cggcgcacaa aaggcacggc cttgactgca ccgattaccg cgccaaatcc cgcagctatc
     2701 gttacgaacc aaatatagag aacgcgcggg tgccgctcgc tcccacgctc gatcagttgg
     2761 tggctgtctg cttgatcgcg cccaatgttg aaaatgtctt gcagttgctg gtccgtgagc
     2821 gcatgcaagt ctggccttac ctggtcgttt tgaccgtcgc tttcatcctg tttcagccac
     2881 tctgcaatcg gatcgtcggc atgcactggt gcgcttagtg ccagcaaaat caagagcacc
     2941 gcagccaaca cgcactttgt agtcggcgct gatcgcaaac gctcctgcca ccaatgcggc
     3001 ttgcttaacc gattgacgtt cacagcactt ccgttgaggg tgctagtttc tgcctgatgg
     3061 ttatgcttgc agatgtcgcg gtggtcgtcc ggcgccgtaa ggctttggct tgaggatcac
     3121 cttttgcagg ttccccggca gaacatatcg gcccacgtac caatgcgcct cgacgttagc
     3181 tccgtaggac tgatcgatcc cgctcgacat cgtatttgcc acgaactcgc caagccccca
     3241 cagtcgtaac tgccgcgcac gtaacagacg gacgaacgga ccagctagct cttggtcctc
     3301 gatgacgata ggtctcgcat gttggtggag agcagtagtc aattgtgacc tacgagagac
     3361 gcgcaccggg atcggcggcg aggacttcgg aggatcggcg tggatgtaga acatgccgac
     3421 atccttgtgg gcaggatgtg ggcgtgcaga aggcagaccc aaaaaatgcc agccgctgtc
     3481 gatggtaacc ctgataatga gtaaattttc tttggttggg gcggagagca agctggtcaa
     3541 acttagaacg cctgtccgat gtcgagccga cgaaaggccc gcgcctcgaa cagccgatga
     3601 gcctccctca caggccgcag cgagatcgcc gatacgttcg ccgccaacgt ccggcccatc
     3661 atcaaagaaa tccaggcgag cggggtcacc agcatgcgcg gaatcgcccg cgccctaacg
     3721 gcccgcggcg tcaagaccgc tcgtggtggt ggtgattgaa gcgacgtgca ggtggccgcc
     3781 atactgcggc gttaggcccg tcgactcgcc ggacggcgcc gagccttccc ccttccccag
     3841 caccgccacg ctctcggcgc cacctagact agtaggccgt gtactcatta agccccacct
     3901 cgcggacgtc agcgcccggc ggttcgcaga atggtgaggt ttctatgagg tcgctcgatg
     3961 gagacgagcc tcgaccacgc agatgagcgc gacctcatag aaaccttcgt tgaacgaagc
     4021 cgct
//
LOCUS       DATLJT010000038         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3447861,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000038 DATLJT010000000
VERSION     DATLJT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447861"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VM910_00770"
     CDS             complement(<1..305)
                     /locus_tag="VM910_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI61118.1"
                     /translation="MRYSKEHKQETHARIVKKASVRLREKGAHGIGVADLMKEAGLTH
                     GGFYAHFDSREALVIEAFTYASSRAREHWRKLAQETPPEKRLALIVDSYLTSLHRDNP
                     "
     gene            472..1242
                     /locus_tag="VM910_00775"
     CDS             472..1242
                     /locus_tag="VM910_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVI61119.1"
                     /translation="MNMLNNHCSVERDARGVVRFTICNAGSLNILGTAVINDVREGLD
                     TLAKDPTIRVLVVAGQSEKSMIGGADIKEMATLDQKTAEAFITRLRDLCEAVRAFPAP
                     VIARMPGWCLGGGLEVAAACDFRIAAHDAKFGMPEVRVGIPSVIHAALLPRLIGWGRA
                     RWLVLTAENIDASTALAWGLVDVVAPQGGLDAAVERTVKLLLECGPEALRSQKALLRQ
                     WEELPLRESIDLSIGVFGRSFLTDEPTRLMQGFVNRKR"
     gene            1382..2578
                     /locus_tag="VM910_00780"
     CDS             1382..2578
                     /locus_tag="VM910_00780"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVI61120.1"
                     /translation="MAATSDPVVIVSAARTPLGRFMGELSPLAAHKLGSHVIGAALER
                     AKLKPEKVDEVFMGNVLPAGQGQAPARQAARGAGLPDATGATTINKVCGSGMKATMLA
                     HDIIHAGSADIVVSGGMESMSNAPYLLAKARSGYRAGHDRIIDHMMMDGLEDAYESGR
                     SMGDFGEATAEAYQFTRKDQDTYAMETLTRARKAVESGAFRAEIAPITLVEKAGPRVI
                     ANDEHPLKVDPAKIPGLKPAFRSNGTITPAASSANADGAAALVLAKRSLAIREGLPIF
                     AEIKGHATHSQEPQWFTTAPIPAIRKLLDKVGWSVADVDLFEINEAFAVVAMAAQKDL
                     GIPRDKLNINGGACALGHPIGATGARLIVTLLHAMEANNLRRGVAALCIGGGEATAIA
                     IERVAA"
     gene            complement(2638..>3393)
                     /locus_tag="VM910_00785"
     CDS             complement(2638..>3393)
                     /locus_tag="VM910_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVI61121.1"
                     /translation="DPERSEEILMKAVPRSRRQVYKMRPIIEAVVDKGSFFEMNANFG
                     RPMIVGLARLQGRAVLVLASDPFHYGGSWTAEACQKVVRWVDFAEIFHLPIVYLMDCP
                     GFMIGLEAEKAATIRHGVRAMAAVNQTTVPWCTVIVRNSFGVAGVVHQPADRFSIRYA
                     WPSAYWGSLPLEGGIEAAYRAEIDAAPDPAAKMKEIEDRLNKLRSPLRSAEKFWVEEI
                     IDPRKTRSLLCEVARLAEPLRTPGLAKNMTIRP"
BASE COUNT          657 a         1068 c         1047 g          621 t
ORIGIN      
        1 ggattatcgc gatggaggga tgtcagatag gaatccacga tcagtgccag acgcttctcc
       61 ggcggcgtct cctgagccaa cttgcgccag tgctccctcg cgcggcttga cgcgtaggtg
      121 aaggcctcga tcaccaacgc ctcgcgagaa tcgaaatggg cgtagaaacc gccatgggtc
      181 agccccgcct ccttcatcag gtcagcgacg ccgatgccgt gcgcgccctt ctcgcgcagc
      241 ctcaccgagg ccttcttcac gatccgggcg tgggtttctt gcttgtgctc ttttgaatat
      301 cgcatggtcg tcctatggaa tgtctatggt catataatag cacgagaacc gacaaaaagt
      361 gcaccaattt cttgaattct gctccacctg ccggccttcg acctgccttg acaaaccgcg
      421 gttttggccg ggaagtgttg tcattcccgc cccgcacgca tgagaccagc catgaacatg
      481 ttgaacaacc attgtagcgt cgaacgtgac gcgcgcggcg ttgtccggtt tactatctgc
      541 aatgccggct cgctcaacat cctggggacg gctgtcatca acgacgtccg cgaggggtta
      601 gacaccctgg ccaaggatcc gacgatccgc gtgctggtgg ttgccggcca aagtgagaaa
      661 agcatgatcg gcggcgccga catcaaggaa atggcgacgc tggatcagaa aacggccgag
      721 gcctttatca cccgcctgcg cgatctctgc gaggccgtgc gggcgtttcc ggcgccagtg
      781 atcgcgcgca tgcccggctg gtgtcttggc ggcggattgg aggtcgctgc agcctgcgac
      841 ttccgcattg cagcgcacga tgcaaagttc ggcatgccgg aggttcgggt cggcatcccc
      901 tcggtgatcc acgcggcgct cctgccccgc ctgatcggct ggggccgcgc ccgctggctg
      961 gtcttgactg cggaaaatat cgacgcgtca accgcactcg catggggcct cgtcgatgtg
     1021 gtcgcgccgc aaggcgggct cgatgccgct gtcgaacgca cggtcaagct attgctggag
     1081 tgcgggccgg aggcgctgcg ctcgcaaaag gcgctgctca ggcaatggga agaattgccg
     1141 ctaagggaat cgatcgacct gagcatcggc gtcttcggca ggtcgtttct gaccgacgag
     1201 ccgacgcgtc tgatgcaggg ttttgtcaac cgcaagcggt aggtacctcg gccgattcag
     1261 gaggctcaat tcgcctcatg ggatcacgac attttgtcat gggacgcatg tgttgcaata
     1321 ttcgcgacgc atcatatgat aggtatcata tatcctatcc gaaatcctgt ggagcgttgc
     1381 catggccgcc acttccgatc ccgttgtcat cgtttccgcc gcccgcactc cgctcggccg
     1441 cttcatgggc gagctgtctc cgctggcagc ccacaagcta ggttcgcatg tgatcggcgc
     1501 ggcgctcgag cgcgcaaaac tcaagccgga gaaggttgac gaggtgttca tgggcaacgt
     1561 gctgccggcc ggacagggcc aggcgccggc ccgtcaggcg gcacgcggcg ccggcctgcc
     1621 cgacgccact ggcgccacca cgattaacaa ggtgtgcgga tccggcatga aggcgaccat
     1681 gctggcacac gacatcatcc acgcgggttc cgcagacatc gtcgtgtccg gcggcatgga
     1741 gagcatgtcg aacgcgccct atctgctcgc aaaggcacga tccggctacc gagcaggcca
     1801 cgaccgtatc atcgatcaca tgatgatgga tgggctcgag gacgcctacg agagcggccg
     1861 ctcgatgggc gatttcggcg aggcgaccgc ggaggcctat caattcacgc gcaaggacca
     1921 ggacacttac gcgatggaga cgctgacgcg cgcgcggaaa gccgtcgaaa gtggagcctt
     1981 cagggccgag atcgcaccta tcacgcttgt cgaaaaggcc ggtccacgcg tcatcgcaaa
     2041 tgatgagcac ccgctgaagg tcgatcccgc caagatccca gggctcaagc cggcattccg
     2101 gtccaacggt acgatcacgc ctgcggcgtc ctcggcgaat gccgacggcg ccgcggcgct
     2161 tgtgctcgcc aagcgctcgc tcgcgatccg tgagggtctg ccgattttcg cggagatcaa
     2221 gggtcatgcg acccacagcc aggaaccgca atggttcacc accgcaccga tccctgcgat
     2281 ccgcaagctg ctcgacaagg tcggctggag cgtcgcagac gtcgatctct ttgaaatcaa
     2341 cgaagccttc gcagtcgtgg cgatggccgc gcagaaggac cttgggattc cccgcgacaa
     2401 gctcaatatc aatggcggtg cctgcgcgct tgggcatccc atcggcgcga ccggcgcccg
     2461 gctgatcgtg acgctcttac acgcaatgga agcaaacaac ctcaggcgcg gcgtggccgc
     2521 gctctgcatc ggcggcggcg aggcaaccgc gatcgcgatc gagcgtgttg cagcctgaca
     2581 gcgaatcgta attggcaaat agcgagtggc ggagatttcc tgttcgctac tcgcctgcta
     2641 tggcctgatg gtcatgttct tcgccaggcc gggcgtgcgc agtggctcgg caaggcgcgc
     2701 aacctcacac agcaacgacc gtgtcttgcg cgggtcgatg atctcctcga cccagaattt
     2761 ctctgcggaa cgaagcggcg atcgcaactt gttcaggcga tcctcgatct ccttcatctt
     2821 ggccgcagga tcgggcgccg catcgatctc ggcgcgatag gccgcttcaa taccgccctc
     2881 cagcggcaga gaaccccaat aggcagacgg ccaggcatag cggatggaaa agcgatccgc
     2941 cggctgatgc acaacaccgg caacaccaaa ggaattgcgc acgatgacgg tgcaccaggg
     3001 caccgtggtc tgattgacgg cggccatggc gcgcacgcca tggcggatgg ttgcggcctt
     3061 ctcggcctct agcccgatca tgaagcccgg gcagtccatg agatagacga tcggcagatg
     3121 gaaaatctcc gcaaaatcga cccagcgcac caccttctgg caggcctctg ccgtccagga
     3181 gccaccgtaa tggaacggat cgctcgcaag cacgagcacc gcgcggccct gaagccgcgc
     3241 cagcccgacg atcatcgggc ggccgaaatt ggcgttcatc tcgaaaaacg agcccttgtc
     3301 gacgacggcc tcgatgatcg ggcgcatctt gtagacctgg cggcggctgc gcggcaccgc
     3361 cttcatcagt atttcctcgc ttcgttcggg atc
//
LOCUS       DATLJT010000039         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_461114,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000039 DATLJT010000000
VERSION     DATLJT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461114"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM910_00790"
     CDS             complement(<1..692)
                     /locus_tag="VM910_00790"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666343.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HVI61122.1"
                     /translation="MAERQLKLGAFMRPVSIHTGAWRYPGAWPDANFNFPHLRQLIRK
                     LEAGKFDAFFMADHLAVLNMPINALKRSHTVTSFEPFTLLSALSGATEHIGLIATGST
                     TFDEPYHIARRFASLDHISGGRAGWNIVTTSNPDAALNFGLDDHMEHAERYKRAREFY
                     DVVTGLWDSFADDAFVRDVEQGLFFDPARMHVLDHKGKYLKVRGPLNIARPVQGWPVI
                     VQAGASEDGKQLA"
     gene            complement(894..1640)
                     /locus_tag="VM910_00795"
     CDS             complement(894..1640)
                     /locus_tag="VM910_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI61123.1"
                     /translation="MTDAAQAMTDDKPEEKEADHHPYFRPKDAATLILVDRAGAVPKV
                     LVGKRHDNVVFMPGKFVFPGGRVDKLDNRVPVAASIPAELEQNLLKGSPRITASRAKS
                     LAIAAIREACEETGLCLGRKADRAPARLEGAWKPFADAGLLPDPSGLFLIARAITPPG
                     RVRRFDTRFFTADASAITHRVEGVIHADAELVELVWVELGSKPLADLHPMTKNVLGEL
                     ENRLATGPLRHDAAVPFFHFYGGKMQKDVL"
     gene            complement(1674..2114)
                     /locus_tag="VM910_00800"
     CDS             complement(1674..2114)
                     /locus_tag="VM910_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HVI61124.1"
                     /translation="METVSSATKVWTRPAVSAEKRDLWTAMKRGFRSRCPRCGQGKLF
                     RAFLKVDDHCSVCGLDFTPHRADDLPAYLVIVIVGHIVVPTALWIETDYAPPVWLQLA
                     IYLPFTLVASLALLQPVKGAVVGLQWALRMHGFDDSPPEGIPPV"
     gene            complement(2102..4423)
                     /gene="rnr"
                     /locus_tag="VM910_00805"
     CDS             complement(2102..4423)
                     /gene="rnr"
                     /locus_tag="VM910_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HVI61125.1"
                     /translation="MKQKHDNGFPSRDAIVAYIRNNPGKVGTKEIAREFGLKNADRAE
                     LKRVLRDLADEGTIKKQGKKISEGATLPATVLADVTGRDADGELIASPAEWDEVEGGE
                     PPKIRIQNLRHARPGTAAGVGDRALLRVERTDDDGVSYRGRVIKVIDHAKHRVLGIFR
                     ALPDGGGRLVPVDKKQAGRELNIAKADALGAGDGDLVSVDLVRSRAYGLASGKVRERL
                     GSIASEKAISLIAIHAHDIPLEFSGSALQEAERAAPATLKGREDWRHVPLVTIDPPDA
                     KDHDDAVHAEADHDPNNKGGYVIHVAIADVAFYVRPGSALDRDAAIRGNSVYFPDRVV
                     PMLPERISNDLCSLVPGEPRGALAVRLVIGSDGRKRSHSFHRVLMRSAAKLNYAQAQA
                     TIDGRPDDITGPLLDPILKPLYAAYALIKRARDERDPLDLDIPERKILLKRDGTVDRV
                     IVPERLDAHRLIEEFMILANVAAAETLEKKSLPLIYRVHDEPTLEKVHALQEFLKTLD
                     VPFAKAGALRPSLFNRVLTLVAGQDYEQLVNEVVLRSQAQAEYSAENYGHFGLNLRRY
                     AHFTSPIRRYADLVVHRGLIRALGLGEGALPDSETAEHLSEIAAEISVTERRAMKAER
                     ETADRLIAHHLVDRVGATFQGRISGVTRAGLFVKLDESGADGLVPIRSLGTEYFNYDE
                     SRHALVGSRSGTMHRLGDVVDVRLVEAQPIAGALRFELLSDGEVVPRGIRRDFAARGA
                     RAKERQRRDNRAKPRKSDKKKSGKSRRGRSWKP"
BASE COUNT          795 a         1364 c         1451 g          841 t
ORIGIN      
        1 gcgagctgct tgccgtcttc ggacgcacca gcctgcacga tcaccggcca gccctggacg
       61 gggcgcgcga tgttcagagg accgcgcact ttgagatact tgcccttgtg atcgagcaca
      121 tgcatccggg ccgggtcgaa aaagaggcct tgctcgacgt cgcgcacaaa ggcgtcgtcg
      181 gcaaaggaat cccacaggcc cgtcaccacg tcgtagaatt cgcgggcgcg cttgtagcgt
      241 tcggcgtgct ccatgtgatc gtcgaggccg aaattcagcg cggcatccgg attcgaggtg
      301 gtgacgatgt tccagcccgc gcgaccgccg gagatatggt caagcgaggc gaagcggcgg
      361 gcgatgtggt agggttcgtc gaatgtcgtc gagccggttg cgatgaggcc gatgtgttcg
      421 gtcgcgcccg aaagcgccga gagcagcgtg aacggttcga acgaggtcac cgtgtggctg
      481 cgcttgagcg cattgatcgg catgttcagc acggcgagat gatcggccat gaagaaggcg
      541 tcgaatttcc cggcctccag cttccggatc agttgcctca gatgcggaaa attgaagttg
      601 gcgtctggcc atgcgcccgg atagcgccat gcgccggtgt gaattgagac cggccgcatg
      661 aaagcgccga gcttgagttg acgttctgcc atagccccgt ttccgccatt tgttgcaggt
      721 cacgaataaa tggggagtgc gcaggggagt accatcgccg ccccacggaa caattttgca
      781 aattgaccgg cgtcgggaga acaaacccgt catttgcgag cggagcaatc gagaaccgtg
      841 gaaaggagtg tgcattgctt cgggcttgcg cctccctgca atgacgccgg aaactagagg
      901 acatccttct gcatcttgcc gccatagaaa tggaaaaacg ggaccgccgc gtcatggcgg
      961 agcggcccgg tagcgagccg gttttccaat tcgcccagca catttttggt catcggatgc
     1021 agatcggcga gcggcttcga gccgagttcg acccagacaa gttcgacgag ctcggcatcg
     1081 gcatggatca cgccctcgac gcgatgggtg atggcagacg catccgccgt gaaaaagcgc
     1141 gtatcgaaac gcctgacgcg accgggcggg gtgatcgcgc gcgcgatcag aaagagtccg
     1201 gacggatcgg gcaaaaggcc agcgtcggcg aacggcttcc aggcgccttc gagcctcgct
     1261 ggagcccgat cggccttgcg gccgaggcag aggccggttt cttcgcaagc ctcgcggatc
     1321 gccgcaatcg cgagcgattt ggcacgggag gccgtgattc tggggctgcc cttaagcagg
     1381 ttctgctcca gctccgccgg gatcgaagct gcgaccggta cgcggttatc caatttgtcg
     1441 acgcgcccgc cgggaaagac gaacttgccg ggcatgaaca ccacattatc gtggcgcttt
     1501 ccgaccagga ctttgggcac ggcaccggcg cgatcgacga ggatcagcgt cgcggcatcc
     1561 tttggccgaa aatagggatg gtgatcggct tccttctctt ccggcttgtc gtcggtcatg
     1621 gcctgtgcgg cgtcggtcat ttccttcccc agatacctag atcgtctgct cgatcagacg
     1681 ggcggaatgc cctcaggcgg actatcgtca aagccatgca tgcgcaacgc ccattgcaag
     1741 cccacgaccg cgccctttac cggttgcaat agcgccaggg atgcgacgag ggtgaacggc
     1801 agatagatcg caagctgcag ccagaccggc ggggcgtaat ccgtttcgat ccagagcgcc
     1861 gtcggcacca cgatgtggcc gacaatgacg atcacgaggt aagccggcag atcgtcggcg
     1921 cggtgcggtg tgaagtcgag cccgcagacc gagcagtggt cgtcgacctt gaggaaggcg
     1981 cgaaacagct ttccttggcc gcaacgagga cagcggctgc gaaatccacg cttcatcgcc
     2041 gtccacaggt cgcgcttctc ggcggacacg gcgggccgcg tccagacctt ggtggcactg
     2101 ctcacggttt ccatgacctg cctctcctgg atttgccgga ctttttcttg tccgacttgc
     2161 gcggcttggc gcggttgtcg cgtcgctggc gctctttggc gcgtgcgcct cgcgcagcga
     2221 aatcgcgcct tatgccgcgc ggcacgacct cgccgtcaga taagagttcg aaccgcagcg
     2281 cgccagcgat cggctgagct tctaccagac ggacgtccac gacgtccccc agccggtgca
     2341 tggtaccact gcgtgagccg accagcgcat ggcggctctc gtcgtagttg aagtattccg
     2401 tacccaggga gcggatcgga accagcccgt cggcgccact ctcgtccagc ttgacgaaca
     2461 ggccggcgcg ggtgacgccg gaaatacggc cctgaaaagt cgcgccgacc cggtcgacga
     2521 gatgatgggc gatcaggcgg tcggcggtct cgcgctcggc cttcattgcc ctccgctcgg
     2581 tgacggaaat ctccgctgcg atttcgctca aatgttctgc cgtctcgcta tccggcagcg
     2641 cgccttcgcc gaggcccaag gcgcggatca gtccgcggtg cacgacgagg tccgcatagc
     2701 ggcggatcgg cgaggtgaaa tgcgcatagc gacggagatt gaggccgaaa tggccgtaat
     2761 tttcggcgga atattcggct tgcgcctgcg agcgcagcac cacttcattg acaagctgct
     2821 cgtagtcctg acccgcgacc agcgtcagca cgcgattgaa cagcgacggc cgtaacgcgc
     2881 cggctttcgc aaagggcacg tcgagcgtct tcaggaattc ctgcagcgcg tgaaccttct
     2941 cgagcgtcgg ctcgtcgtgc acgcggtaga tcaggggcag tgacttcttt tcgagcgttt
     3001 cggctgcggc gacattggcg aggatcatga actcctcgat cagccggtgt gcgtcgagtc
     3061 gctccggaac gatgacgcga tccacggtgc cgtctcgctt gagcaggatc tttcgctcgg
     3121 gaatgtcgag gtcgagcgga tcgcgctcgt cgcgcgcgcg cttgatgagc gcgtaggccg
     3181 catagagcgg cttcagaatc ggatcgagga ggggccccgt gatgtcgtcc ggacggccgt
     3241 cgatggtggc ctgcgcctgc gcgtagttga gcttcgcggc cgagcgcatg aggacgcgat
     3301 gaaagctgtg cgagcgcttg cgtccgtcgg acccgatcac gagccgcacc gcgagcgcgc
     3361 cgcggggctc gccaggcacc agcgagcaga gatcgttgga gatgcgctcc ggcagcatcg
     3421 gcacgacgcg atccggaaaa taaaccgaat tgccgcggat cgccgcgtcg cggtcgagcg
     3481 cagaacccgg ccgcacatag aaggccacat cggcgatcgc gacatggatg acgtagccgc
     3541 ccttgttgtt gggatcgtgg tcggcttcgg cgtgcaccgc gtcgtcatgg tccttcgcgt
     3601 ccggcggatc gatggtgacg agcggcacgt ggcgccagtc ctcgcggcct ttcaacgtcg
     3661 caggcgcggc ccgttcggct tcctgcagcg cggacccaga gaattcgagt ggaatatcgt
     3721 gggcgtgaat ggcgataagg ctgatcgcct tctcgcttgc aatcgagccc agtctttccc
     3781 tgaccttgcc cgaggcaagt ccataagctc gggagcggac gaggtcgaca ctgacgagat
     3841 cgccgtcccc agcgccaagc gcgtctgcct tcgcgatgtt caattcgcgg ccggcctgct
     3901 tcttgtcgac aggaacgagc cgcccgccgc cgtcgggcag agcgcggaaa atgccgagca
     3961 cgcggtgctt ggcatgatcg atgaccttga tgaccctccc gcgataggaa acgccatcat
     4021 catctgtacg ctcgacgcgc aacagcgcgc ggtcgccgac gccggcggcc gtccccggcc
     4081 gggcatggcg aagattttga atgcggattt tcggcggttc gccgccttcg acttcgtccc
     4141 attcagcggg gctggcgatc aactcgccgt cggcatcgcg tccggtgacg tcggccagta
     4201 cggttgccgg cagcgttgcg ccttcgctga tcttcttgcc ctgcttcttg atcgtgccct
     4261 catcggcaag atcgcgcaga acgcgcttga gttcggcgcg atcggcattt ttcaggccga
     4321 actcgcgcgc gatttccttg gtcccgacct ttccgggatt gtttcggatg taggcgacga
     4381 tggcgtcccg cgacggaaag ccattgtcgt gtttttgttt cacttaacct cgtgcctttc
     4441 cggcgctctt t
//
LOCUS       DATLJT010000040         1546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_231297,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000040 DATLJT010000000
VERSION     DATLJT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231297"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VM910_00810"
     CDS             complement(<1..361)
                     /locus_tag="VM910_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HVI61126.1"
                     /translation="MKVHLWLAGIVLLGVVAPAAAAESEAERLKQAQGLFQPLPRDMA
                     RPDAPITPQRVDLGRLLFFDPRLTVDADLSCSSCHQPARYGTDGLPKSIGVRQRPHPR
                     HAPTILNAGLNFIIHWRG"
     gene            complement(443..646)
                     /locus_tag="VM910_00815"
     CDS             complement(443..646)
                     /locus_tag="VM910_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61127.1"
                     /translation="MDRLIGCLIGGAVHQPFEGNVTTAHIISTDLSQIYVAASWAGTV
                     ELAIVAAVGLGLWLRERKFAQRI"
     gene            complement(988..>1546)
                     /locus_tag="VM910_00820"
     CDS             complement(988..>1546)
                     /locus_tag="VM910_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI61128.1"
                     /translation="HALRWSRLWPFLLGAAFGVPLGVAILGWARPDYVRVTVGVVLVL
                     YSTYSLARPAMKPVKRGGATADAGVGFLNGILGGLTGLAGILTTIWCGLRGWPKDEQR
                     AIFQPIGVAIFAMSGLWLGINGAVSTDTIWFFVIGLPALLVGTWLGLKLYGHLDEAGF
                     RKLVLVLLLVSGIALVAPALTPLFG"
BASE COUNT          338 a          496 c          457 g          255 t
ORIGIN      
        1 cgccgcgcca gtgaatgatg aaattgagcc cggcattgag gattgtcggg gcatggcgcg
       61 gatgcggtcg ctgcctgaca ccgatcgact tgggcagccc gtccgttccg tagcgcgctg
      121 gttgatggca actggaacaa ctgaggtcgg cgtcgaccgt cagtcgagga tcgaagaaga
      181 gcagccgacc gagatcgaca cgctgcggcg taatcggcgc gtccggcctc gccatgtccc
      241 gcggaagcgg ctgaaacagt ccctgagcct gcttgagccg ctcggcttcg ctctccgctg
      301 cggctgccgg cgcaacgacg cccagcagca cgatgccggc caaccagagg tgtaccttca
      361 tcacctatct ccctcggcca ttaaccccgt taccgctccc ggcttcgaag ctttcacgaa
      421 atcttgatcc gctgcgcgga gatcagatgc gctgggcgaa cttgcgctcc cgaagccaga
      481 ggccgagacc gacggcggcg acgatggcaa gttccaccgt ccctgcccaa gaggcggcaa
      541 catagatctg agacaggtct gtcgagatga tgtgagcggt cgtcacattc ccctcaaagg
      601 gttggtgtac cgcaccgccg atcagacatc cgatgagtct atccatagct ctctccttcg
      661 cggtacatac atcgaggagt agagcgaagc ggtctacatt taaggcaaaa ttaaacgtcg
      721 gcctacgtta atcacagatc ggtgaggccc gaaatcacag atcggtgagg cccgaccttc
      781 cgtatcgagc gctgccgtag aactcatcga catcggcggc acttgcaggc cgagtgccca
      841 ctcgatgaag cttatgtcgc gctatgtggt gtaatcagag agacggccag gcgcggcatt
      901 cgctcgcgat gatgtgcgta ctaaaaacct cgttccgtac ttcgcggcca gcagtcagca
      961 gcacgctgtg cacttcgtcg ccgaccatca cccaaaaagc ggtgtgagag cgggcgctac
     1021 caacgcgatt cccgagacca gcaacaggac gaggaccagc ttgcgaaagc cggcttcatc
     1081 cagatgtcca tagagcttta atcccagcca cgtcccgacc agcaatgccg ggaggccaat
     1141 gacgaagaac caaatggtgt cggtcgatac cgcaccgttg atcccaagcc acagaccgct
     1201 catggcgaag atcgccactc caatcggctg gaagatcgcc cgctgctcgt ccttgggcca
     1261 accacgaagg ccgcaccaga tggtcgtgag aatgccggcg aggccggtga ggccgccgag
     1321 aatgccgttc agaaagccca cgcccgcgtc cgccgtagca ccgccgcgct taacgggctt
     1381 catcgcgggc cgggccaggc tataagtgct gtagagcacg agcaccacgc cgacggtgac
     1441 gcggacgtag tcaggacgcg cccatccgag aatcgccacg cccaacggga ctccaaaggc
     1501 cgcccccaac aggaatggcc acagccgact ccagcgcagc gcgtgg
//
LOCUS       DATLJT010000041         1983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3907472,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000041 DATLJT010000000
VERSION     DATLJT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907472"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VM910_00825"
     CDS             <1..270
                     /locus_tag="VM910_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61129.1"
                     /translation="ALAQVKRGAVELLTPRMDLAAAEALADKLAGGHWTHDYALTATE
                     ARALGLPVKVGMPQEVLALMKLYPQPIQQSGIEYLPIDIPRQRRV"
     gene            371..1351
                     /locus_tag="VM910_00830"
     CDS             371..1351
                     /locus_tag="VM910_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61130.1"
                     /translation="MKRRDVITLLGGAAAAWPLAARAQQAGKLPTIGFLGSSTLSAMS
                     QWTAAFVQRLRELGWVEGRNVAIEYRWAEGRADRLSEIAAEFVRLKVDVIVTYATTGT
                     LAAKQATSAIPIVFAIAGDPVGTGLVASLARPSGNVTGLSLQLTEITGKRLELLREAV
                     PDLRRLAILGNVGNPFTVLEIDEVQAAARTLGLEVHTPEIRRAQDIAPAFEALRGRAD
                     AIYVSTDPLVFTNLTRINTLALGSRLPTIYTSRDYVAAGGLMSYGPNYPDLWRRAADY
                     VDKILRGAKPGDIPVEQPTKFDLIVNLTTAKTLGLTIPESFLLRADEVIE"
     gene            complement(1762..>1983)
                     /locus_tag="VM910_00835"
     CDS             complement(1762..>1983)
                     /locus_tag="VM910_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61131.1"
                     /translation="GTFLAIRSRVAWSFMPCQPPRARRHGLPGLPGTFLAALRKQDGI
                     AARALEFLILTATRTGETLGATWDEVDMR"
BASE COUNT          376 a          619 c          636 g          352 t
ORIGIN      
        1 gctctcgccc aggttaagcg aggggcggta gagctattga cgccgcggat ggacctagcg
       61 gctgcggaag ccctcgcgga taagcttgcg ggtggacatt ggacgcatga ctacgcgctt
      121 actgcaactg aagcccgcgc gcttgggctc cccgtcaaag tgggtatgcc gcaagaggta
      181 ttggcgttga tgaagctcta cccgcagcca attcaacaat ctggcatcga gtacttgccc
      241 atcgacatcc cgcggcagcg ccgagtctga cggtgatgca aatatgactg atcccattat
      301 cgcgcgcgac ccgaagggat tgagccggaa gtgccgcccc caattcggcc agagccgacg
      361 aggtgatcga gtgaaacgcc gcgatgtcat cacgctgctc ggcggtgcgg ctgccgcttg
      421 gccgctcgca gcgcgcgcgc agcaggcggg gaagctgccg accatcgggt tcttgggctc
      481 aagcacgctt tccgccatga gccaatggac tgctgctttt gtacagcgac tgcgcgaact
      541 cggctgggtc gaaggtcgca atgtggcgat cgagtatcgc tgggcggagg gacgcgccga
      601 ccgcttgtcc gagatcgcgg ccgagttcgt ccgcctcaag gttgatgtca tagtcacgta
      661 cgcaaccaca ggaaccctcg cggccaagca ggcgacttcg gccatcccga tcgtcttcgc
      721 catagcgggg gacccggtcg gcaccggcct ggtggcgagt ctggcacgac cgagcggcaa
      781 cgtcaccggc ctatcgctcc agctgaccga gattactggc aagcgtctcg aattgttgcg
      841 cgaggctgtc cccgatctgc gccggttggc gatcttgggc aatgtcggca atcccttcac
      901 cgtgctggag atagacgagg tgcaggcggc ggcccgcacg ctcggtcttg aagtccacac
      961 acccgaaatc cggcgagcgc aagatatcgc gcccgccttc gaggcactca ggggccgcgc
     1021 ggacgcgatt tatgtctcga cggacccact tgtatttacc aacctgactc gcatcaacac
     1081 cttggcgcta ggctcgcggc tgccgacgat ttacacttcg cgggactacg tcgcagcggg
     1141 aggtctgatg tcctatggac caaactaccc ggacctgtgg cggcgcgccg ccgattatgt
     1201 cgacaagatt ttgcgggggg caaagccggg cgacattccg gtcgagcagc caaccaaatt
     1261 cgatctcatt gtcaatctga ccactgcgaa aacgctcggc ctaaccatcc cagagtcgtt
     1321 cctgctgcgc gccgacgagg tgatcgagtg aagcggcgcg acttcatcac gctgctcggc
     1381 ggcgcggcag cagtgtggcc gctcgcagcg cgcgcacagc accgacgatg ccggtggttg
     1441 ggtatctcgg catcggatcg ctcgaaccga acgcgccgtc gatggccggc ttgcgcaagg
     1501 gtctgaacga gcaaggcttc gtcgaagctc agaacgtaat catcgaaacc cgccttgcgg
     1561 cggaaggtca ttatgaccaa ctgctagcgc tcgcattcga tctcgtcggc cgcccagtgg
     1621 cggtccttgt cgcgactggc agcgctggct ctgcgcaagc cgccaaagca gcgaccacgg
     1681 ccatcccgat cgtgtttgcg aacggcagcg acccggtcag ggtcggtctc gttgcaagta
     1741 tgaatcggcc gggcggcaac gctacctcat atcgacttcg tcccaggtcg cgcccaacgt
     1801 ctcgccggtg cgggtggcgg taaggatgag aaattcgagg gcacgggcgg cgatgccgtc
     1861 ctgctttcgc aacgcggcca agaaggtacc gggaagaccg ggaaggccat gccgtcgcgc
     1921 tcgcggcggc tggcatggca tgaaggacca tgccacacgg ctacggatcg caaggaatgt
     1981 tcc
//
LOCUS       DATLJT010000042         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2988470,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000042 DATLJT010000000
VERSION     DATLJT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988470"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..710
                     /locus_tag="VM910_00840"
     CDS             177..710
                     /locus_tag="VM910_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61132.1"
                     /translation="MSGNDSLRRELDAASTIIFPSAYLAKLDELKKRFQHSIRVLPEA
                     AGKIARFNCFAYAFGVWDKPRYERLVDQTNNSALMNSGFIMERLDRGELIEIDAAEAQ
                     PNDIVVYFAGDRLRHAGRIITASGKLTIRSKWGGNEVHEHELWEVPLEHGNQVRFFRP
                     PDPKALLDRLEAAQKPA"
     gene            941..1273
                     /gene="erpA"
                     /locus_tag="VM910_00845"
     CDS             941..1273
                     /gene="erpA"
                     /locus_tag="VM910_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVI61133.1"
                     /translation="MTTASITVTERAAERIAQIVCNEPDKKMFRISVEGGGCSGFEYR
                     FDLVPKAEPEDAVIEHADAKILVDSLSLVYLAGSEIDYVDDLISASFKIKNPNATAAC
                     GCGTSFSV"
     gene            1330..1431
                     /locus_tag="VM910_00850"
                     /pseudo
     CDS             1330..1431
                     /locus_tag="VM910_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
     gene            complement(1624..1899)
                     /locus_tag="VM910_00855"
     CDS             complement(1624..1899)
                     /locus_tag="VM910_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVI61134.1"
                     /translation="MSIAATIREKLMIALRPTRLDVINESYVHAGHRSSPGTGESHFR
                     VLVMSPEFAGKSRVDRHRLVNDTLAAELKGQVHALAIQAYAPGEEMR"
     gene            complement(2197..2343)
                     /locus_tag="VM910_00860"
     CDS             complement(2197..2343)
                     /locus_tag="VM910_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HVI61135.1"
                     /translation="MGIRDRPTSPRSPWQNAYAERLIGTLRRDCLDHVLIFGVQHLRQ
                     ILTS"
     gene            3460..3747
                     /locus_tag="VM910_00865"
     CDS             3460..3747
                     /locus_tag="VM910_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61136.1"
                     /translation="MLGEQRKKRTTEARDLIFKRARWSRLSGEWSEDDYDVLAAGEVV
                     GRINKVKAAPEGSPWFWTLAYGYHYDCTPIHGYEATREAAMAAFAKSWRRE"
BASE COUNT          843 a         1130 c         1152 g          714 t
ORIGIN      
        1 cgggatcggc tgctacttcg atgatcccgt tcacgaggtg ttcggcatct cttcgcgcga
       61 ctggcagagc ttctaccact tcacggtagg tggcgccgtc gaggacaaac ggctcacgac
      121 gttgccagcg tacaagttcg aggaaaaacg atgagctcgc accggggacc gccgaaatgt
      181 ccggaaacga cagcctgagg cgtgagctag atgccgccag cacgataata tttccatcgg
      241 cctatctggc gaagctggat gaattgaaaa aacgcttcca gcattcaatc cgcgtgcttc
      301 cagaagccgc ggggaagatc gcgcgtttca attgcttcgc ctatgccttc ggggtgtggg
      361 acaaacctcg ctatgagcgc ctggtggatc agacgaacaa ctcggcattg atgaactcgg
      421 gcttcatcat ggagcggctt gaccgcggcg agttgatcga gattgatgct gccgaagcgc
      481 aaccgaatga catcgtggtt tatttcgccg gagatagact caggcacgcg gggcgcatca
      541 tcacggcctc aggcaagctt actatccgtt ccaagtgggg cggcaacgaa gtccacgagc
      601 acgaactttg ggaggtgcca ctggagcacg gtaatcaggt acgtttcttc cgcccgcccg
      661 atccgaaagc cctgcttgat cgcctagaag cggctcagaa accggcatga ccatgaaaac
      721 ttcatgccgt caggagtttc atcctcctga ctgggcgcat ccagcgcgat cattccgcgc
      781 tgacattagg cgattctgtt agtaatcaat acggatgagg ttctcggtac acacaggggc
      841 cgaaatccta tccatgggcg gagtcctgcc gcctgattga cggccagccg caaggccacc
      901 gtccctcgca acccgtatcg caaaaaggga atcacgagcc atgacgaccg catccatcac
      961 cgtcaccgag cgcgcggccg agcgcatcgc acaaatcgtc tgcaacgaac ctgacaaaaa
     1021 gatgtttcgg atcagcgtcg agggcggcgg ctgttcgggc ttcgagtacc gcttcgatct
     1081 ggtgcccaag gcggagcccg aagacgccgt gatcgagcac gctgacgcga agatcctagt
     1141 cgattcgctc tcgctcgtct atctggcggg ctcggagatc gactacgtcg acgacctgat
     1201 aagcgcgtcg ttcaagatca agaatccgaa cgcgaccgcc gcctgcggct gcggcacaag
     1261 cttctcggtc tgaagccgat ccgtcacggg agcgctcgcg tgctcgacag tcgcgccaac
     1321 ggcaataaga tgacgctcga tacgatccgc tccgatttcg agttcctcga cgactgggag
     1381 gaccgctacc gctacgtgat cgagctcggg cgcggcttgc ccccttgccc gaggcctacc
     1441 gcacggaccg cggccaggtg tggctcgcaa ccgaggccga gccccagggc attgtcgcgt
     1501 tagcgcagta aaggcgtttt cgggtttaga cggagcctgc aaaatgctac agcgccatgg
     1561 aacgaaactg gcggagtttg gaagcaaatc ccgttaacgt gacaatgccc attggcgtgg
     1621 ctctcagcgc atctcttccc ccggcgcgta agcctggatg gcgagggcgt ggacctggcc
     1681 tttcagctcg gccgcgagcg tgtcgttgac cagccggtgg cggtcgaccc gcgacttgcc
     1741 ggcgaactcg ggcgacatca cgagtacgcg gaaatggctc tcgcccgtgc cgggcgagct
     1801 ccggtggccc gcatgcacat acgactcgtt gatcacgtcg agccgggtcg gccggagcgc
     1861 gatcatcagt ttctctcgga ttgtcgccgc gatagacatt caggaagctg tcccttccga
     1921 aaagcatgtc cggacatatt ggtgatacac gggcgtgcag catcgactcg cggtgcgcgc
     1981 ggaaggcgac caacacgtca catgcctcct cgcggaggca acccatcact accacaccgt
     2041 gagcaatcga aaaagcccgg cctgtcgttc ccgaaaatta tatccgcgca tactgatgat
     2101 gcaggccgga cagaatgggc gtggcgacaa tggttccgca tcgctggacc gcccgccgca
     2161 gcggcgcatc cttgtccaat gataaatgcg tgcgggtcac gaggtcagga tctgccgcag
     2221 atgctgcacg ccgaagataa gaacgtgatc caaacagtcg cgccggagcg ttccaatcag
     2281 tcgttcagca tatgcattct gccacggcga tctcggtgat gttggacgat cacgaatacc
     2341 catcgtccga atccgacgcc tgaaggcctg tccgtaagca ccatcgttgt cgcgcaccag
     2401 ataggccgca gccgcgttcc acggaaatgc cttcacgatc tgttggaaag gttctcgacc
     2461 tcaagcccga cgccgcgact tgagcagatc ggcgataaat attccaatca ggtagatgat
     2521 cgcaaacatc gcattggatg atggtctgat tctccctcac agcgcgaatc ctaccggatc
     2581 gaattttcgg gaacgacagc ctctgccgca cgcgttcccg gcccacggtt aggaaaaggt
     2641 agcgcccctc gaaaacgacc ccctcacaat gcctaatttg tcgcaaggca gggcgccgcc
     2701 aacagcagta atcttagagc caaaatttag aaccagggag cacgtcagaa aagggagaga
     2761 gctatgatcg ccatgttcga agggcccgac agaaacagaa acataataat cgccgtagcg
     2821 gtggtggtag tcgctgtgat catctttctt ttcgcggggg gctatttgtc aggaataatc
     2881 tgaggaggca cattcacccg agcttccata ggggccgcct cagttgggca gtggctggtt
     2941 tccgagccag atttgagatt gggttggctg ttgttgcgtg gccaagttca cggtgatcgc
     3001 tggcgacggt cgcgggccgg aagacccgca cgtcctcgaa gcgtgctacc atttcaggcg
     3061 gctggtcgta gaaattctgc gcgccatcgt tcgcggaaac gattacggat cgcgtgtcgg
     3121 tcgctcatcg atcctgcgct cgccgagctg cagaaaaaaa ttgcggcaga gcgcgacgat
     3181 actaccgaca gctacatgac cgagatcagc gacattgtgc tcgggagcct catggtcgcg
     3241 accgagctgt gttgcacgga tggcttcgca aaggcccgac tatccgaccg cacgactgaa
     3301 ctcagaacct atatcgaaga gcacatcatc ggctgtgagc gtcgatcgcg cgcgagcggg
     3361 ggagcttcct gcgccgcttg atgcaaaagc gcttcccacc ggagccgaga cggccgaaga
     3421 ggtagggcgc cgcatgtcct cgccgaaaac accggaggcg tgcttggaga gcagaggaaa
     3481 aagcgcacga cagaggcgcg cgacttaatt ttcaagcgcg cccgatggag ccgcctctcg
     3541 ggcgagtgga gcgaggacga ttacgacgtg ctcgccgccg gtgaggtggt cggccgcatc
     3601 aataaggtca aggcggcgcc tgagggctcg ccatggttct ggacacttgc ctatggatat
     3661 cattacgact gcacgccgat acatggctac gaggcgacac gcgaggctgc gatggcggca
     3721 ttcgcaaaga gctggcggcg ggagtgagat atggccaaac cgaaggcgaa cgcgcccaaa
     3781 ttcccgacag taacgatggt ggattcccgg accctcaccc gggcggatgg gaccacctc
//
LOCUS       DATLJT010000043         6331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2988506,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000043 DATLJT010000000
VERSION     DATLJT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2988506"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VM910_00870"
     CDS             complement(<1..674)
                     /locus_tag="VM910_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI61137.1"
                     /translation="MADRLTGLEVFAKVAATGSLSAAGRAMGLSQTMVTKHLAALEAR
                     LGAKLFHRTTRRLSITDAGRSYLESCERILAEIEAADAAVSADRIEPRGLLRLNVPLV
                     FGVRQIAPRLGEFARRHPRITVELGLNDRLVDLAEEGWDLAIRIGSLRDSSLMARRLA
                     PCRMTICAAPSYLAARGTPRTVAELAGHNCLGYTLAQSGADRWLMGKRAEISVAVSGN
                     LRANNG"
     gene            781..1224
                     /locus_tag="VM910_00875"
     CDS             781..1224
                     /locus_tag="VM910_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI61138.1"
                     /translation="MIDSRTAPYAALLLRLSLGALFLAHAGLKFFVFTPAGTAKFFGS
                     VGLPGELAYVVILAEVLAAVALILGVWTRVVALLATPILLGAIFTVHGSAGFFFSNPH
                     GGWEFPAFWTVALLVQALLGDGALAVGSPFAHPAGGLRLSSAHSN"
     gene            1339..2481
                     /gene="solA"
                     /locus_tag="VM910_00880"
     CDS             1339..2481
                     /gene="solA"
                     /locus_tag="VM910_00880"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HVI61139.1"
                     /translation="MRSCEIGIIGLGLMGSAALYELTRRGCDALGFDPLVIGEARGSS
                     HGSCRVFRRFNFESEAYTALSDRAFEGWLALESESGRTLLKPSAVLEAGPPGCQLVAN
                     SRAAALAAKSRVTGPRSGAEANAAFPAFSLPFDWDVIVQESGAILLAEEAMRAFRDGA
                     KGRIIQAAAQMRATPAGIRIVTASEEVMAEKVIVAAGPWVAGLIPGLKSHLTVTRQAV
                     GWFAPVRPDTTRYGEFPIFILETARGMIYGFPDFEGRGIKAAQHDHGAIAGADEWEPS
                     ATDAELDGVAATLAELVPGAAGPITDRDICLYTNAARADMRVDGGNEFIIDRLPQDPR
                     IIVASPCSGHGAKFATAIGAMLVDLALDPKLAAPDPFRLDRFSSFA"
     gene            2680..3708
                     /locus_tag="VM910_00885"
     CDS             2680..3708
                     /locus_tag="VM910_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI61140.1"
                     /translation="MTIAVSRREFVKTSAMAATWLAAAPAIIGRAEAATLKLKCSSSL
                     PNDPKYANGRVYYDNLVKNLKANGLSEQVDVTFFPDNQLGQEIDVINSVKLGVIDLMV
                     SGSSISANLVPLVGTFDLGYLFASFPQQTRAFEGGAAKPIEDALLKGAGIRIIAWAYN
                     FGARSVLSKKPVKTPEDLAGLKIRTLPNPIITECLRLMGAAATPLAFGEIYTALQAGV
                     LDGLEHDPPTILASKFFETSKNYALTQHIFSPLAIYFSEATFTRMDPKLREGFLDAAQ
                     KAAADSRAHGLAVEKEALATLMEKGVTVVECDKEAFRKRVAPQTDNFVKAHPEAKSVV
                     DAIRATSA"
     gene            3720..5561
                     /locus_tag="VM910_00890"
     CDS             3720..5561
                     /locus_tag="VM910_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVI61141.1"
                     /translation="MRSAAPATSWIMAALLAISDAVAAIMLAADLLVVCASVMLRYWF
                     NAPVEWADDVARGLMVGSSFFGAASALARSENPGVHFFIDKLPAALRALVDSVGALLM
                     ALIAGYVAFNALKLGGLTAGQTTGSGLPLELTFYPMGVGALFMTVFALDIFRARPVRD
                     MTLGVIATVLIAGLYLAWDYLAPSSVPSSGMLMLAGFFITLFGGLPIGFALALAALIF
                     IWVEGTLPGVIFAQQMARGIDNFVLLAIPFFILVGYLMEANGMSVRLIELLQRAVGHM
                     RGGLNVVMVTSMVLFSGISGSKMADVAAVGSVLIPAARRTRQNPGSAVALLAASAVMA
                     ETIPPCINLIILGFVANLSIGGLFVAGILPAALMALALIAVSVIAGKRPAAEEAQLRT
                     EVKGLWSGAIASFGLIFMIFFGFKSGFATATEISAFAALYAIVIGSVLFRELSLKAAA
                     HSFVQAATRSGLVLFIVAAAQSLAFILTLQQVPHAVGDIMLSLSKTNGVWLFMLLSIA
                     VLIVMGSVLEGAAALIIFGPLLLPVAVKLGVDPLHFGVVLVIAMGIGLFAPPLGLGLY
                     GACLIGNVPIERTVKPILGYLGLLLVCLLVVAFVPIISTALPRAFGY"
     gene            5574..>6331
                     /locus_tag="VM910_00895"
     CDS             5574..>6331
                     /locus_tag="VM910_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI61142.1"
                     /translation="MKVLLTHPPEFRRQYYGERSLKGLQAIADVKLHRGDQALDAKGL
                     LAAARDVDIIVSDRMTEGRGEIFRELPHLRAFVRCAVDIRNVDVAAASASGVLVTHAS
                     PGFVAAVAELALGFMVDRSRGVSRATADYHAGRKPEVRMGRQLAGSKVGIIGYGAIGR
                     YLARLVKALGMEVLVADPHVTVSEPQIRHLALDEMLGRADYVVCLAIANEETENLIGQ
                     ATLARMQKHAFFINLSRGNLVDEAALAAALRENL"
BASE COUNT          993 a         2065 c         2046 g         1227 t
ORIGIN      
        1 tcgccgttgt tcgcgcgcag gtttcccgaa acggcaacgc tgatctcggc ccgcttgccc
       61 atcagccagc ggtcggcgcc ggattgcgca agcgtgtagc cgaggcaatt gtgaccggcg
      121 agttccgcga ccgtgcgcgg cgtgccgcgg gcggcgagat aggagggggc cgcgcagatc
      181 gtcatgcggc acggcgcaag ccgccgcgcc atcaggctcg agtcgcgcaa actgccgatc
      241 cggatcgcaa ggtcccagcc ctcttcggcg agatcgacca gccggtcatt gaggccaagc
      301 tcgaccgtga tacgcggatg gcggcgcgcg aactcgccaa gccgcggcgc gatctggcgg
      361 acgccgaaca ccagcggcac gttgagccgc agcagcccgc gcggctctat cctgtcggcc
      421 gagacggctg catccgcggc ttcgatctcg gccaggatgc gctcgcagga ttcgaggtag
      481 ctgcgtccgg catcggtgat cgaaaggcgc ctggtggtgc ggtgaaagag ctttgcacca
      541 aggcgcgctt ccagtgctgc gagatgcttc gtgaccatgg tctgcgagag gcccatcgcg
      601 cgccccgccg ccgacaggct gccggttgcg gccactttcg caaacacctc aaggcccgtg
      661 agccggtccg ccatggaaca tctcattccc tgagtgtgag ctgtatttac cgaataccgg
      721 attatctgtc gaactggaat ggatcatacc tcccgggacc cccaaatcaa ggagattgcc
      781 atgatcgatt cccgaacggc gccctatgcg gcgctgctcc tgcgcctcag cctgggcgcc
      841 ttgtttcttg cccacgcggg cctgaagttc ttcgtgttca cgccggccgg cacggcgaaa
      901 ttcttcggca gcgtcgggtt gccgggcgag cttgcctatg tggtgatcct tgccgaagtg
      961 ctcgccgcgg tcgcgctgat cctcggcgtc tggacgcgcg tcgtcgcgct gttggccacg
     1021 ccgatcctgc tcggagccat ctttaccgtg catgggtcgg ccggattttt cttctccaac
     1081 ccgcatggcg gctgggaatt tccggccttc tggaccgtgg cgctgcttgt gcaggctctg
     1141 ctcggtgacg gcgctttggc tgtgggctcg cccttcgccc atccggccgg cggcctgcgg
     1201 ctgtcgagcg cgcattcgaa ctgaacggcg cccacggcgc gagacggatg cggtgcgagg
     1261 gtggcttgcc gcgttcgcag accgtaggat gggttgggcg aagccaaacc catcgacgtg
     1321 ccgtgaagag aacgcatcgt gcgaagctgt gagatcggca tcatcggcct cggcctgatg
     1381 ggaagcgcag cgctttatga actgacccgc cgcggctgcg acgcgctcgg gttcgatccg
     1441 cttgtgatcg gcgaggcgcg aggctcgtcg cacggttcgt gccgggtctt tcgccgcttc
     1501 aacttcgaga gcgaagccta caccgcgctc agcgaccgtg ccttcgaggg ctggctcgcg
     1561 ctcgagtcgg aaagcgggcg gactcttctg aagccttctg ccgtgctcga ggcggggcct
     1621 ccgggctgcc aactggtcgc aaactcgcgc gccgcggcct tggcggcgaa gagccgcgtc
     1681 acggggccgc gcagcggagc cgaggccaac gcggcgtttc ctgccttcag cctgccgttc
     1741 gactgggacg tcatcgtgca ggagagcggc gccattctgc tggccgagga ggcgatgcgc
     1801 gccttccgcg atggcgcgaa gggccgcatc attcaggcgg cggcgcaaat gcgcgccacg
     1861 ccggccggca tccgcatcgt gactgcaagc gaagaggtaa tggcggaaaa ggtgatcgtc
     1921 gccgccggtc cgtgggtcgc cggcctcatc cccggtctga aatcccacct gacggtgacg
     1981 cgacaggctg tcggctggtt cgcgcctgta cggccagata ccacgcgcta cggcgagttt
     2041 ccgattttca tcctcgagac cgcgcgcggg atgatctacg ggtttcccga tttcgagggc
     2101 cgcggcatca aagccgcgca acacgaccac ggcgcaatcg caggcgccga cgaatgggag
     2161 ccgtcggcga ccgatgccga attggacggc gtagcggcga cgctcgccga gctcgttccg
     2221 ggcgcagccg gtccgatcac cgatcgcgat atctgtctct acacgaacgc ggccagggcc
     2281 gacatgcgcg tcgacggcgg taacgagttc atcatcgatc gattgccgca ggacccgcgc
     2341 atcatcgtcg cctcgccctg ttcgggtcac ggcgcgaaat tcgcaaccgc cattggcgcg
     2401 atgcttgtgg atctcgcgct cgatccgaag ctcgcggcgc cagacccgtt tcgactggac
     2461 cgcttttcca gctttgcctg aatgttgcga actcacgccg ttcgcgatgg tggccgttta
     2521 agatcgatgc gacatcggca ttgatccata ccgcaactgg atcggtcctt cgcagcctgc
     2581 cacccttgcc tgcaccttct ctttcgccct acagtcgcac aacaagcggt cgcggcgacg
     2641 cgcgcgatcg gaaagaaaag catctgggag aaaagcgcca tgaccatcgc cgtcagccgt
     2701 cgcgagttcg tgaaaacgtc ggcgatggcc gcaacatggc ttgccgcggc acccgcaatc
     2761 atcggccgcg ccgaggctgc gacgttgaag ctcaagtgct cgtcgtcgct gccgaacgat
     2821 cccaaatacg ccaacggccg cgtctattac gacaatctgg tcaagaacct gaaagccaac
     2881 gggctctccg agcaggtcga cgtcaccttt tttcccgaca atcaactggg tcaggagatc
     2941 gacgtcatca actcggtgaa gctcggggtc atcgacctca tggtgtcggg ctcctcgatc
     3001 tcggcgaatc tggtgccgct ggtcggcacc ttcgacctcg gctatctctt tgcgagtttc
     3061 ccgcagcaga ccagggcgtt cgaaggcggc gcggcaaagc cgatcgagga tgccctgctc
     3121 aagggcgccg gcatccgcat catcgcctgg gcctataatt tcggcgcgcg cagcgtgctc
     3181 tcgaagaagc cggtgaagac gccggaggac ctcgcaggcc tcaagatcag gacgctgccc
     3241 aatccgatca tcaccgaatg cctgcgcctg atgggcgcgg cggcgacccc gctcgccttc
     3301 ggcgagatct acaccgcgct gcaggccggt gtgctcgacg ggctcgagca cgatccgccg
     3361 accatcctcg ccagcaagtt tttcgagacc tcgaagaact acgcgctgac ccagcacatc
     3421 ttctcgccgc ttgcgattta tttcagcgag gcgaccttca cccgcatgga tccgaaactg
     3481 cgcgaaggat ttctggatgc cgcgcagaag gccgccgccg acagccgcgc ccatggactt
     3541 gcggtggaaa aggaagcgct cgcaaccctc atggagaagg gcgtcaccgt ggtcgaatgc
     3601 gacaaggagg ctttccgcaa gcgcgtcgcg ccgcagaccg ataacttcgt caaggcgcac
     3661 ccggaggcca agtccgtcgt ggacgcgatt cgcgccacat ccgcctgagg caggttggca
     3721 tgaggagtgc cgctccggcg acgagctgga tcatggccgc gctgcttgcg atcagcgacg
     3781 ccgtcgccgc gatcatgctg gcggccgatc tgctcgtggt gtgcgcgtcg gtgatgctgc
     3841 gctactggtt caacgcaccg gtcgaatggg ccgacgacgt tgcgcgcggg ctgatggtcg
     3901 gttcgagctt cttcggcgcg gccagcgcgc tggcgcgctc ggaaaatccc ggcgtgcatt
     3961 ttttcatcga caagctgccg gccgctctgc gcgccctggt cgattccgtc ggcgcgctgt
     4021 tgatggcgct gatcgccggc tatgtcgcgt tcaatgccct caagctcggc ggcctgacgg
     4081 ccgggcagac cacgggttcc ggcctgccgc tggaactgac cttttatccc atgggcgtcg
     4141 gtgcgctgtt catgacggtt tttgcgctcg acatttttcg cgcgcgcccg gttcgcgaca
     4201 tgacgttggg ggtcattgcg accgtgctga tcgccggtct ctatctcgcc tgggattatc
     4261 tggcgccttc gtcggtgccg tcgtcgggca tgctgatgct ggccggtttc ttcatcacgc
     4321 tgttcggcgg acttccgatc ggctttgcgc tggcgctggc cgcgctgatc ttcatctggg
     4381 tggagggtac gctgcccggc gtcattttcg cccagcagat ggcacgcgga atcgacaatt
     4441 tcgtgctgct ggcaatcccg ttcttcatcc tggtcggcta tctcatggaa gccaacggca
     4501 tgtcggtacg cctgatcgag ctcttgcagc gcgcagtcgg ccacatgcgc ggcggtctca
     4561 acgtcgtgat ggtcacctcg atggtgctgt tctccggcat ctccgggtcc aagatggccg
     4621 atgttgccgc cgtcggctcg gtcctgattc ctgccgcgcg tcgaaccagg caaaatccgg
     4681 gaagcgcagt ggcgctgctc gccgcctccg ccgtcatggc ggagaccatt ccgccctgca
     4741 tcaatctgat catcctgggc tttgtcgcca atctttcgat cgggggcctg ttcgtcgccg
     4801 gtattctgcc ggccgccctg atggcgcttg ccttgattgc ggtgtcggtc attgcgggaa
     4861 agcggccggc ggcggaggag gcgcaattgc ggacggaggt gaagggcctg tggagcggcg
     4921 caatcgcctc cttcgggctg atcttcatga tcttcttcgg cttcaagagc ggctttgcca
     4981 ccgcgaccga aatctcggcg ttcgccgccc tctacgccat cgtcatcggc agcgtgctgt
     5041 ttcgcgagct cagtctcaaa gccgccgcac acagcttcgt ccaggcagcg acgcgctcgg
     5101 ggctcgttct ctttattgtc gccgccgcgc agtcgcttgc cttcatcctg acgctgcagc
     5161 aggtgccgca cgcggtcggc gatatcatgc tgtcgctgtc gaaaacgaac ggcgtgtggc
     5221 tgttcatgct gctgtcgatc gcggtcctga tcgtgatggg ctcggtgctc gagggcgccg
     5281 cggcgctgat catcttcggc ccgctgctct tgccggtcgc ggtcaaactc ggcgtcgatc
     5341 ccctgcattt tggcgtcgtg ctcgtgattg ccatgggaat cggcctgttc gcgccgccgc
     5401 tcggcctcgg actttacggc gcctgcctga tcggcaacgt gccgatcgaa cggacggtga
     5461 agccgatcct gggttatctc ggattgttgc tggtatgcct cttggtggtg gctttcgtgc
     5521 cgattatctc cacggcgctg ccgcgcgcgt tcggctattg acggaaaaga cacatgaaag
     5581 tcctgctgac gcacccgccc gagttccgcc gccaatatta cggcgagcgt agcctcaaag
     5641 gcctgcaggc gatcgccgac gtcaagctgc accggggcga ccaggcgctg gatgccaagg
     5701 gcctgctcgc ggccgcgcgc gacgtcgaca tcatcgtgtc cgaccgcatg accgagggca
     5761 ggggcgagat ctttcgcgag ctcccgcacc tgcgcgcctt cgtgcgctgc gcggtggaca
     5821 tccgcaatgt cgatgtcgca gcggcatcgg ccagcggcgt gctggtgacc cacgcttcgc
     5881 ccggcttcgt cgcggcggtc gccgagcttg cgctcggctt catggtcgat cggtcgcgcg
     5941 gtgtgtcgcg cgccaccgcc gactaccacg ccggccgcaa gcccgaggtc aggatgggcc
     6001 gccagcttgc cggcagcaag gtcggcatca tcggttatgg cgcgatcggc cgctatctcg
     6061 cgcgccttgt gaaagcgctg ggcatggagg tgctggtcgc cgatccccac gtcaccgtca
     6121 gcgagcccca gatcaggcat ctcgcgctcg atgaaatgct tggtcgtgcc gactatgtcg
     6181 tttgccttgc gatcgccaat gaagaaaccg aaaacctgat cgggcaggcg acattggcgc
     6241 ggatgcagaa gcatgccttc ttcatcaacc tgtcgcgcgg caatctcgtc gacgaagcgg
     6301 cgctggccgc agccttgcgc gagaacctca t
//
LOCUS       DATLJT010000044         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3218352,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000044 DATLJT010000000
VERSION     DATLJT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218352"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1410
                     /locus_tag="VM910_00900"
     CDS             313..1410
                     /locus_tag="VM910_00900"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVI61143.1"
                     /translation="MTRAMEPELGDSKKKMVSGTYLRNAWYVAAWSDDLADGQLLSRT
                     ILKERIVLFRKSDGHVGALQDRCPHRFAPLHMGKIVGGDRVQCPYHGLEFDSSGACVL
                     NPHGTKNIPPRARVRSYPVTEKHKAVWIWMGEQPPDLAKVPDFSVLDNVPEMHATKRD
                     RITIRANYELIIDNLLDLSHTSYLHDGILGNSDTVESEITVEQDGDDVVVGRYARNAT
                     APGLFAILMPSTPERVDKFTRMRWMAPSNLRLLTGICPPGAVPESGTGYHAIHMLTPE
                     SDKTTHYFFTAVRFNVLTADDRLNAQIQDKISTTRRFAFEEQDAPVIEAQQEMIDAAA
                     TAVDPVILAIDVGPVRYKQILQKLIHAEQRQ"
     gene            complement(1534..2247)
                     /locus_tag="VM910_00905"
     CDS             complement(1534..2247)
                     /locus_tag="VM910_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HVI61144.1"
                     /translation="MSTRGHVVAVTCLALEARIALGPGVAVICNHASELVAALEAAAK
                     RGAAGIISFGIAGGLAPDLGAGDWVVGSGVRTEQGHFPTDRGWARALLEALPSAVHAE
                     IVGADAPIAEPSEKRRLHLQTGAVAVDTESHLAARIAAAHRIPFAACRAVIDPAHSVL
                     PPAAMYGLRHDGTADVFAVLRSVMRQPSQLPALTRTALDARIAAAALRRGRRLLGAGL
                     GFPYFSEPVLDGRMAVEMG"
     gene            2820..>3418
                     /locus_tag="VM910_00910"
     CDS             2820..>3418
                     /locus_tag="VM910_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HVI61145.1"
                     /translation="MPTWTPDPSFYPSPRQAAKAPPETLAYVAAFDPDRKSPDRIAVV
                     DVDPKSSSYSKIIGNVAATEVGDEFHHFGWNACSSCLCPNAPHPHVERRYLVVPGLRS
                     SRIYILDTKPDPRAPKIVKVIEPAELADKTGYTRPHTVHCGPGGIYVAALGNKDGKAP
                     GGVFVMDHESFEPLGRWEVDRGPQQLGYDAWWHLGYDTMV"
BASE COUNT          669 a         1114 c         1032 g          603 t
ORIGIN      
        1 ccccggcgag gttttcaccg gggccttagc gctcccgttg gctcttgggg ggcagtgggg
       61 ccttccggga acgccacaag gttatcgttc ggcgagcggt ttggttcccg ccgatataaa
      121 tcgatcgttc gcgaccgcca cagtcagcgc aaagcgcgtc gggcggcttt gcgccattcg
      181 ccgtgccaag acggcggctt gacccgcaac gatggcccga aagataatcc gctgcattcg
      241 cgcaggtatg ccgcctcgcc atcgcggagg tcgcgcttcc gcttgatcgg cctcagacat
      301 ggaggacccg cgatgacgcg cgcaatggag cccgagcttg gcgacagcaa aaagaaaatg
      361 gtcagcggga cctacctccg caacgcgtgg tatgtcgccg cctggtccga tgacctcgcc
      421 gacggtcaat tgctcagccg caccatcttg aaagagcgga ttgtcctttt ccgaaaatcg
      481 gatggacatg tcggcgcgct gcaggatcgc tgtccacacc gattcgcacc tctgcatatg
      541 ggcaagatcg tcggcggcga tcgcgttcaa tgtccttatc atgggttgga attcgactcg
      601 tccggcgctt gcgtcttgaa cccgcatgga acgaagaata ttcctccccg cgcccgcgtg
      661 cgcagctatc cggtgacgga gaagcacaag gcggtctgga tttggatggg tgaacagccg
      721 cccgacctcg ccaaagtgcc ggacttcagc gtgctcgata atgtccccga gatgcacgca
      781 acgaagcgcg accgcatcac cattcgcgcc aactatgagc tcataatcga taatctgctc
      841 gacttgagcc atacctctta tctgcacgac gggattctcg gcaattcgga cacggtcgag
      901 tcggaaatca ccgtcgagca ggacggtgac gacgtcgtcg tcggccgtta cgcccgcaat
      961 gcgacggcac cggggctatt cgccattctg atgccatcga cgccggaacg cgtcgacaag
     1021 ttcacccgca tgcggtggat ggcgcccagc aatttgcgtc tgttgacggg aatttgcccg
     1081 cccggggcgg tgcccgagag cggaaccggt tatcatgcga tccatatgct cacccccgaa
     1141 agcgacaaga ccacgcacta ttttttcacc gcggtgcggt tcaatgtttt gaccgcggac
     1201 gatcggttga acgcgcagat ccaggacaag atatcaacaa cccgccggtt cgcattcgaa
     1261 gagcaagacg ccccggtgat cgaggcgcaa caagagatga tcgacgcggc ggcgacggcg
     1321 gttgatccgg tcattctagc gatcgacgtg gggccggtgc gctacaagca aatcctgcaa
     1381 aaattgattc atgccgagca gcggcaatag gcgatgcgcg ccggagccat cggccgggcg
     1441 gatcagggtt taactcgaga cgttgacaag gtgatcgcgc cgtcgacgtc acggcgcggt
     1501 aaagccaagc tcttctggca aagagacgac ggctcaaccc atctcgaccg ccatgcggcc
     1561 gtcgagcaca ggctcgctga aatacgggaa acccaatccg gcgcccaata gccggcggcc
     1621 gcgccgcagc gccgccgccg cgatgcgggc atcgagggcg gtgcgcgtaa gcgcaggaag
     1681 ctggctgggc tgtcgcataa ccgagcgtag taccgcgaag acatccgctg tgccgtcgtg
     1741 gcgtaatccg tacatcgctg ccggcggaag cacgctgtgg gccggatcga tgaccgcgcg
     1801 gcaggcggca aatggaatcc gatgcgcggc ggcgatcctg gctgcaagat gtgattcggt
     1861 atcgacggcg acggcaccgg tttgcagatg aagccgacgc ttttccgaag gctccgcgat
     1921 cggggcatcc gccccgacga tctccgcgtg gaccgcgctc ggaagcgcct cgagaagagc
     1981 gcgtgcccat ccacgatcgg ttggaaagtg tccctgctcg gtcctgacgc cggatccgac
     2041 gacccagtcg ccggcgccaa gatcgggcgc caatccgccg gcaataccaa aactgatgat
     2101 gcccgcagcg ccacgcttgg ctgccgcctc gagcgcggcc acgagctcgg aggcatgatt
     2161 gcagatcacc gccacgcccg gccctagggc gatgcgtgcc tccaacgcaa gacaggtgac
     2221 ggcgactacg tgccccctag tcgacattca actctcgttc tgttcgcgta gcgcgtccgc
     2281 gtatttcggc ggtaatcttc cccagaaagc gaaattggtc aacggccttg ccctcgatct
     2341 gcccacccgc gatggtcgcc tcgctgcgaa gacacgcctc cccggtgcgg cacctcggcg
     2401 tgcatttttc cgcgcttggc tggccatccg tcggccgact cgacctccgt attggacaac
     2461 tttatcactg ttcctcggac gatttgatgt cccgcccatg ccgcttttgg tgtgccccag
     2521 tgccatgacc gccgcggcgg tgcctgctgt cgcaacccag ccagaatcgc catcacgcac
     2581 aagaacggac cgggtacaag cgtccattcc ggcttgatat atgcgacagg atccaccgag
     2641 ctacaatggc cacgaatgcg cggggcgtgc ccactcgcgc ctatctcgca gaccgcttcc
     2701 gagttcggag aaaaagggac gctgcacgtg agcgattcgg tcgcgctcgg atccgcttgc
     2761 gcgatgcgac gcaagcgaga agatgaagat gcacgccggt ctcgagagga gaaaatcaca
     2821 tgcccacctg gacgcctgat ccaagttttt atccctctcc gcgccaagcg gcgaaggcac
     2881 cgcccgaaac gctcgcctat gtcgccgcct tcgatcccga ccgaaaatcc cctgaccgca
     2941 tcgccgtcgt cgacgtcgat ccgaaatcgt cgtcgtactc caagatcatc ggcaacgtgg
     3001 cggcgaccga ggtgggcgac gagtttcatc atttcggctg gaacgcctgc tcgtcgtgtt
     3061 tgtgcccgaa cgcgccgcac ccccatgtgg agcgacgcta cctcgtggtc cccggcctgc
     3121 gctcctcgcg catctatatt ctcgacacca aacccgatcc gcgagcgccg aaaatcgtca
     3181 aggtcatcga gccagcggag cttgcggaca agaccggcta cacccgcccg cataccgtcc
     3241 actgcggacc cgggggcatc tacgtcgccg ccctcggaaa caaggacggc aaggcgcccg
     3301 gcggcgtctt cgtcatggat cacgagagtt tcgagcccct cggccgctgg gaggtcgacc
     3361 gcggaccgca gcagctcggt tacgacgcct ggtggcacct cggctacgac acgatggt
//
LOCUS       DATLJT010000045         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1839696,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000045 DATLJT010000000
VERSION     DATLJT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839696"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VM910_00915"
     CDS             complement(<1..171)
                     /locus_tag="VM910_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HVI61146.1"
                     /translation="MQPVSIILNVFWILIGGAWMAFGWLVAAIVMALTIIGLPWARAA
                     FNIAAYTLMPFGS"
     gene            complement(318..3413)
                     /locus_tag="VM910_00920"
     CDS             complement(318..3413)
                     /locus_tag="VM910_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI61147.1"
                     /translation="MQGRSSKAATATWRRLTFGQRRDPIFWLIASGCILVAAICVGTM
                     IMIGEFRERALKNGERELENTVMLLTRHFEQQFEDSEAIAAEVIGQMKTSGIDSPEIF
                     KRRMSTPEMHELLHSKVSALSYIGDIAMFDADGTLLNWSRSFPPPRISIADRPYFKIF
                     KTDPQSEPIVAEPARSYVDGNWTTIVAHRLNASDGTLLGVITRRIDPANYERFFASLA
                     LGEGAAISMFHSDGTMLARYPHIDAMIGRKFKDAPLVNRVLAAGGQQTLRVKSPVDGL
                     DRLGAAAQLSHFPIMVVATNTVAAALTDWRAQTRFMVTAAALSATLIACILFLIIRQI
                     NQHNRDAQQRLEAEKHRLDTALNNMTQGLVQYDASARIVTFNQRYIDMYKLSTDVVKP
                     GLHYYDLIQHRKDTGSYDGDVRAFCDPIMRDVAEGKVSRAVMETGDGRAYHIVNKPLK
                     QGGWVATIEDITERRSLEQERDRNYAFLREIIDRIPSQITVKDARSRRYVLANSVAEM
                     QFGMSREGIVGKTCFDLFAKRLAEIITADDDRTILSRDSLFIDEHRWESQALGSRFMT
                     SKRVAIRDNAGEPRYIINVVEDVTERRQANDKIAHMAHYDALTDLPNRALFREQIERE
                     LQKSAHGEQFALLYIDIDEFKGINDSLGHHVGDELLKSVAARIRGCIGDTDLIARLGG
                     DEFAVIQTAVGSKADAVEFVMRLHEAIRQPYQCLTHQLSTDASIGIALAPQDGTDLDQ
                     LIKNADLAMYGAKAEGRRTYRFFEPSMDASAKARLTMQQDLRQAMADGAFEIHYQPLV
                     TLANSEVCGCEALLRWRHPERGMVSPAEFIPLAEDTGLINELGEWVLSTACAEAATWP
                     EHIRLAVNVSPLQLKCQTLALRITSALAASGLKPSRLELEITEAVLIRDNEAALAILH
                     QLREIGVRIALDDFGTGYSSLGYLKRFPFDKIKIDRCFVSDLSETEGSSSIVQAVVNI
                     ASSLNMTTAAEGVETAAQRELLRKLGCTEMQGYLFSAAKPAADIKRFFTASGKNTTAA
                     A"
BASE COUNT          628 a         1042 c         1136 g          698 t
ORIGIN      
        1 tgagccgaac ggcatcaagg tgtaggcggc aatgttgaag gctgcgcggg cccagggcag
       61 tccgatgatc gtgagcgcca tcacgatcgc cgcgacgagc cagccaaagg ccatccaggc
      121 gccgccgatc aggatccaga agacgttcag gataatggag acgggttgca tgcggtcacg
      181 ggcctctgcg caagatcgaa caattctagc agccggcgac gccgaaggcg agggttcggc
      241 cgaggtgacg cgggttgcgg ccgaggctcg cgcggcgtaa cgcgcgcggt tggcctgtcc
      301 gatcaggcac cggaatttta cgctgccgcc gtcgtgtttt tgccggaggc tgtgaaaaat
      361 cgcttgatgt cggcagccgg cttcgccgcg ctgaacaggt aaccctgcat ctcggtgcag
      421 ccgagcttgc gcagcaattc gcgctgcgcg gcggtttcga cgccctcggc ggccgtcgtc
      481 atgttcagcg aggaggcgat gttgacgacc gcctgcacga tcgacgagga accctccgtc
      541 tccgagaggt cgctgacaaa gcagcggtcg atcttgattt tgtcgaacgg aaaacgcttc
      601 aaatagccca gcgaggaata gccggtgccg aaatcgtcaa gcgcgatgcg cacgccaatc
      661 tcgcgcaatt gatgcagaat ggcgagcgcg gcctcgttgt cgcggatcag cacggcttcc
      721 gtgatctcga gctcgagccg gctcggcttc aggccggagg cggccagtgc gctcgtgatc
      781 cgcagcgcca gtgtctggca tttcagttgc agcggcgaca cgttgacggc taggcgaatg
      841 tgctcgggcc aggtcgcagc ctcggcgcag gcggtcgaca gcacccattc gccgagttcg
      901 ttgataagtc cagtgtcttc ggcgagcgga atgaactcgg cgggcgaaac cataccgcgc
      961 tcggggtgac gccagcgcaa caatgcctcg catccgcaaa cctcgctgtt cgcgagcgtt
     1021 accagcggct ggtagtggat ctcgaaggcg ccatccgcca tagcctggcg caggtcctgc
     1081 tgcatggtca gccgcgcctt ggcgctggcg tccatcgacg gctcgaagaa gcgataggtg
     1141 cggcggccct ccgccttggc gccgtacatc gcaaggtcgg cattcttgat gagctggtcg
     1201 aggtcggtgc cgtcctgcgg cgccagcgcg atgccgatgc tggcgtcggt cgagagctgg
     1261 tgcgtgaggc attggtaggg ctgtcggatc gcctcgtgca ggcgcatcac gaactccacg
     1321 gcgtcagcct tgctccccac tgcggtctgg atcacggcga actcgtcgcc gccgagacgc
     1381 gcgatcaggt cggtatcccc tatgcagccg cgaatccgcg cggctacgct cttcaagagt
     1441 tcgtcgccga catggtgccc gagggaatcg ttgatgcctt tgaattcgtc gatatcgatg
     1501 tagagcaggg caaattgctc gccgtgagcc gatttttgca gctcgcgctc gatctgctcg
     1561 cggaacaatg cgcggttcgg caggtcggtg agggcgtcat agtgcgccat atgcgcgatc
     1621 ttgtcgttgg cctggcgtcg ctcggtgaca tcctcgacca cgttgatgat gtagcggggc
     1681 tcgccggcgt tgtcgcggat cgcgacgcgc ttcgaggtca tgaagcggct gccgagcgcc
     1741 tggctttccc aacgatgttc gtcgatgaac aggctatcgc gggacaatat ggttctgtcg
     1801 tcgtccgccg taatgatctc ggccagccgt ttggcaaaca gatcgaaaca ggtttttccg
     1861 acgataccct cgcgcgacat gccgaattgc atctcggcca ccgaattggc cagcacgtaa
     1921 cgacggctgc gcgcatcctt caccgtgatt tgcgacggaa tacggtcgat gatttcacgg
     1981 aggaaagcat aattgcggtc gcgctcctgc tcgaggctgc gccgctcggt gatgtcctcg
     2041 atggtggcaa cccagccgcc ctgcttgagc ggcttgttga cgatgtggta cgcgcgaccg
     2101 tcaccggttt ccatgacggc gcggctgacc ttaccctccg cgacgtcacg catgatggga
     2161 tcgcagaagg cgcggacgtc gccgtcatac gagccggtgt ccttgcgatg ctggatcagg
     2221 tcgtaataat gcaagccggg tttcaccacg tcagtcgaca gcttgtacat gtcgatgtaa
     2281 cgctggttga atgtgacgat gcgcgccgac gcgtcgtact gcacgagacc ctgcgtcatg
     2341 ttgttgaggg cggtgtcgag ccggtgcttt tcggcttcca gccgctgctg cgcgtcgcgg
     2401 ttatgctgat tgatctggcg tatgatcaaa aacaggatgc atgcgatcag cgtggcggac
     2461 agcgccgcgg cagtcaccat gaaccgggtt tgcgcgcgcc agtcggtcaa cgcagcggcg
     2521 accgtgttgg tcgcgaccac catgatcggg aaatggctga gctgcgccgc cgcgccgagc
     2581 cggtcgagtc cgtcgacggg gctctttacg cgcagcgtct gctggcctcc ggcggccagc
     2641 accctgttta ccagcggcgc atccttgaat ttccgtccta tcatcgcatc gatgtgcgga
     2701 tagcgtgcga gcatggtgcc gtcgctgtga aacatcgaaa tagcggcgcc ttcgccaagt
     2761 gcaagcgagg cgaagaaccg ctcgtaattg gcgggatcga tgcgccgcgt tatcacgccg
     2821 agcagcgtcc cgtcggacgc gttcagccgg tgggccacga tggtcgtcca attgccgtcg
     2881 acgtagctgc gcgccggttc ggcgacgatc ggctccgatt gcgggtctgt cttgaatatc
     2941 ttgaagtagg gtcggtcggc gatgctgatg cgcggcgggg gaaagcttcg cgaccagttg
     3001 agcagtgtcc cgtcggcatc gaacatcgcg atatccccga tgtaggacag cgcactgacc
     3061 ttcgaatgca gcaactcgtg catttccggc gtggacatcc gacgcttgaa tatttcaggc
     3121 gaatcgatgc ccgaggtctt catctgcccg ataacttcgg ccgcgatcgc ctccgaatcc
     3181 tcgaactgct gttcgaagtg ccgcgtcagg agcatcacgg tgttttccag ttcgcgctcg
     3241 ccgttcttca acgcgcgctc gcggaattcg ccgatcatga tcatggtgcc gacgcagatc
     3301 gccgcaacaa ggatgcagcc gctcgcgatc agccagaaga tggggtcgcg gcgctgcccg
     3361 aatgtgagcc gccgccacgt ggcggtggcg gcttttgacg atctgccctg aatgctggaa
     3421 atcaacgcgc atttcccctc gaaggggatg aatacgacgc cgaaatggca taagccgtta
     3481 ggaaatccag ttactttcga ttta
//
LOCUS       DATLJT010000046         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_461360,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000046 DATLJT010000000
VERSION     DATLJT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461360"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VM910_00925"
     CDS             <1..559
                     /locus_tag="VM910_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVI61148.1"
                     /translation="LVAEPQANGPAAKAGIESGDVITAVNGESVKDARELARTIGGLA
                     PGTAVKLNVLHKGEDKVINLTLGTLPNQLEAKADNGNDDRGGATRGTDVPKLGLTVAP
                     ANSVAGAGKEGVVVTEVDPKSAAAERGFKEGDVILEVAGKTVASANDVRDAIAAAKND
                     NKNVVLMRVKSGGQSRFVAIPLAKG"
     gene            829..1509
                     /locus_tag="VM910_00930"
     CDS             829..1509
                     /locus_tag="VM910_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI61149.1"
                     /translation="MRLLIIEDDRESADYLVKAFREVGYVADLAGDGEEGLAMAENGD
                     YDVMVVDRMLPRRDGLSLIGALREKNVRTPVLILSALGQVDDRIKGLRAGGDDYLPKP
                     YSFAELLARVEVLSRRNVGPAEETTYRVGDLELDRLSHRVARGKDELTLQPREFRLLE
                     YLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKGFDRPLLHTIRGAG
                     YMIRDGLR"
     gene            1493..2959
                     /locus_tag="VM910_00935"
     CDS             1493..2959
                     /locus_tag="VM910_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI61150.1"
                     /translation="MTAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITE
                     QITTTVNAEASEISDIYNRRGLRGLVFTVDSRALRPGANLYLVTTPAGQAIAGNVGSL
                     APGVMGTEGWSETAYRRLEDQDNTDHRALVNVTQLSGGFRLLIGRDLEERRRLFGIVA
                     KAAQWSILVVVVLGIAGGIFVARRVLQRIDAMTGTTRRIMAGDLSGRLPVGRSGDELD
                     RLAENLNAMLERIEALMVGLKEVSDNIAHDLKTPLTRLRNRAEEALAKSSCEAEYRAA
                     LERTIEESDGLIRTFNALLMIARAESGQARGNMDDFDAAEVANGIHELYEPLAEDDGM
                     TLRVKTAPAPIHANRELISQALANLVENAIKYGKPTSAAQVQGAVVSMDAKQILIEAR
                     REGDKVLLSVTDRGIGIPEGDRRHAVERFVRLDSSRSQPGSGLGLSLASAVATLHGGE
                     LRLDDAHPGLVATLVIPARAGGTDRLAAQTQDVPQKVA"
BASE COUNT          589 a         1003 c         1046 g          554 t
ORIGIN      
        1 acttgtcgcc gagccccagg ccaacggtcc ggcggccaag gccggcatcg agtccggcga
       61 cgtgatcacc gcagtcaacg gcgagagcgt caaggatgcg cgcgaattgg cgcgcaccat
      121 cggcggcctt gcgccgggca cagcggtcaa gctcaacgtg ctgcacaagg gcgaggacaa
      181 ggtcatcaat ctgactttgg gtaccttgcc caaccagctc gaggccaagg ccgacaacgg
      241 caatgacgac cggggcggcg ccactcgcgg cacggatgta ccgaaactgg gcttgacggt
      301 ggcccccgcc aacagcgttg ccggtgccgg caaggaaggc gtggtcgtca ccgaggtcga
      361 tccgaagagt gcggcggccg agcgcggctt caaggaaggc gacgtcattc tcgaggtcgc
      421 cggcaagacg gtcgcttcgg ccaacgatgt gcgtgatgcg atcgcggccg cgaagaacga
      481 caacaagaac gtcgtcctga tgcgcgtgaa gagcggcggt cagtcgcgct tcgtcgcgat
      541 ccccttggca aagggctgac gggttttgcg agatggaggg tgtcgccggc atcagtcgcc
      601 cccgccgacg gctctttccg gggagcgggc gaaagcccgc tcctgcaatc taccttccga
      661 tggaaatacg cccccctccg tcggaagggc ctaagggcgg tggagttccc ccagctccac
      721 cgcctgcttt ttgcgattaa ccgaatggct ctcggtcgcc ttgcatgacg tagccgaccg
      781 ggccatgttg aaggaaggcc gacccacttg accgcaaccc cgccgcagat gcgcctgttg
      841 atcatcgaag acgaccgcga atccgccgac tatctggtga aggcattccg cgaggtcggt
      901 tacgttgcgg acctcgccgg tgacggcgag gaaggtctcg caatggccga gaacggcgac
      961 tacgacgtga tggtggtcga ccggatgctg ccgagacgcg acgggctgtc gttgatcggt
     1021 gcgttgcgcg agaagaacgt ccgcacgccc gtcctcattc tctccgcact tgggcaggtc
     1081 gacgaccgca tcaaaggtct gcgcgccggc ggcgacgact atctgccgaa gccctattca
     1141 tttgccgagc tgttggcgcg ggtcgaagtg ctgtcccgcc gcaatgtcgg cccggccgag
     1201 gaaaccacct atcgcgtcgg cgacctcgag ctcgatcgtt tgtcgcaccg cgtcgcacgc
     1261 ggcaaggacg aactgacgct tcagccgcgc gaatttcgtc tgttggaata cctgatgaag
     1321 catgccggcc aggtggtgac gcggaccatg ctcttggaaa atgtctggga ttatcatttc
     1381 gatcctcaga ccaacgtgat cgacgtgcat atttcgcggc tgcgctccaa aatcgacaag
     1441 ggttttgacc ggccgctgct ccataccatt cgcggcgcgg gatacatgat acgtgacggc
     1501 cttcggtaag ctgatccgaa ccaccgcgtt ccggttgacg ctggtctatt tgttgctgtt
     1561 cgcgcttttt gcggcctcgc tgctcggcta tttcgcctgg aacacacggc gcctgatcac
     1621 cgagcagatc accaccaccg tcaacgccga ggcgagcgag atcagcgaca tctataaccg
     1681 gcgcgggctg cgcgggctcg tcttcaccgt cgacagccgc gcattgcggc ccggcgcgaa
     1741 tctctatctc gtcaccacgc ccgcagggca ggcgattgcc ggcaatgtcg gttcgctcgc
     1801 gccgggcgtg atgggcacgg agggttggtc ggagaccgcc taccgcaggc tcgaggacca
     1861 ggacaacacg gatcaccggg cgctcgtcaa cgtcacgcaa ttgagcggcg gcttccgtct
     1921 tctgatcgga cgcgatctcg aagagcggcg gcggctgttc ggcatcgtcg ccaaggcggc
     1981 gcaatggtcg atcctggtcg tcgtcgtgct cggcatcgcc ggcggcatct tcgtggcgcg
     2041 acgcgtgctg cagcggatcg acgcgatgac cggcaccacg cggcgcatca tggccggcga
     2101 tctgtccggc cgcttgccgg tcgggcgcag cggcgacgag ctcgatcgtc ttgcggaaaa
     2161 tctcaacgcc atgcttgagc ggatcgaggc gctgatggtg ggactgaagg aagtctccga
     2221 caacatcgct cacgatctca agacgccgct gacgcggttg cgaaaccgcg ccgaagaggc
     2281 gctggcgaaa tcgagctgcg aggcggaata tcgcgcagcg ctggagcgca cgatcgagga
     2341 atccgacggc ttgatccgca ccttcaacgc gcttctgatg atcgcgcgcg ccgaatccgg
     2401 acaggcgcgc ggcaacatgg acgacttcga cgccgccgag gtcgccaacg gcattcacga
     2461 gctctacgaa ccgctcgccg aagacgacgg catgacgctg agggtcaaga cggcgccggc
     2521 gccgatccac gccaatcgcg agctgatcag ccaggcgctg gccaatctcg tcgaaaacgc
     2581 catcaagtac ggcaagccta cttccgctgc gcaggtgcaa ggcgcggtcg tcagcatgga
     2641 cgccaagcag atcctgatcg aggcccggcg cgaaggcgac aaggtgctgc tgagcgtcac
     2701 cgatcgcggc atcggaattc cggaaggcga ccgcaggcat gcggtcgagc gtttcgtccg
     2761 gctcgattcg agccgcagcc agcccggttc cggccttggg ctcagcctgg cgtccgccgt
     2821 cgcgactttg catggcggcg agctcaggct ggacgatgct catccgggtc tcgttgcgac
     2881 cctggtcatt ccggcgcggg caggcggcac cgacaggctt gcagcccaaa cgcaggatgt
     2941 gccacagaag gtggcgtgag ctcgatcccg cgagacacgg tcaccttcgc tcgttcccat
     3001 ccgcaaaatt cgtgatgctt tgccatgacg acttgcgaat gcgctcgtgg cccggtcccg
     3061 acgttcgcat tttgctgggg aggcgggttt gcgaatgtta gagtcaacgg acgtcgagca
     3121 gacatcgctg ccagcggagc tttggatttg tcgttcgcat gaaggaatcg cgtcgaaatg
     3181 ggtagcgaac gt
//
LOCUS       DATLJT010000047         1920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2069820,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000047 DATLJT010000000
VERSION     DATLJT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069820"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VM910_00940"
     CDS             <1..699
                     /locus_tag="VM910_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI61151.1"
                     /translation="LPGEAPYFNKDIATGEWSGMCIEMAKDIAGVFDAKVDYVESTYG
                     NSVLDLQANKIDLAFSLNPTPKRALVIDFTHAFYLHGFGMVGAKGFHASNWGELNKPE
                     VRIAVDIGSVHEIAARRFAPNATIIGLKARDDCILAVQSGRADCVILAVNLGLTAVKK
                     NPALGEFQMLHQPRVQLPTCMGVRMEADKRWRDFLDAWVDYNRGIQQIREWLYGGLAI
                     NGVKPEDVPADVEF"
     gene            723..1397
                     /locus_tag="VM910_00945"
     CDS             723..1397
                     /locus_tag="VM910_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI61152.1"
                     /translation="MTGTRYAWDFSFLWSYRGLILIGLGVTIAYTIGTILLGLVVGLV
                     TGLLRLSKNVLITAPLVAYVEVFRCTPLLVQIVWFYYALPVVLGIDIPAHVAATLVLS
                     LYTGAFYAEIVRGGVNSIERGQWDAARAIGMRRAQVMRYVVLPQAIKRMIPPFMNQSI
                     IQLKNTSLVSTIAVADLLYQGTIITAATYRPLEVYTMVAVIYFLVLFPLTLAAQQVEH
                     RLARAD"
     gene            1421..>1920
                     /locus_tag="VM910_00950"
     CDS             1421..>1920
                     /locus_tag="VM910_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61153.1"
                     /translation="MMIEIADLWKSFGALPVLKGVSLKVERGSVVAIIGPSGSGKSTL
                     LRCINLLEEPDRGHIRVGDRERGFGAGVRTKPSDRELSAFRAATGMVFHHFNLFPHMT
                     ALGNVMTGPSIVKGMARHEATEIARALLSKVGLADKAEAYPSHLSGGQRQRVAIARAL
                     AMNPAV"
BASE COUNT          347 a          589 c          591 g          393 t
ORIGIN      
        1 ctccccggcg aggcacccta tttcaacaag gacatcgcga ccggggaatg gtcggggatg
       61 tgcatcgaaa tggccaagga catcgccggg gttttcgacg ccaaggtcga ttatgtcgaa
      121 tcgacctatg gcaactcggt cctcgatctg caggccaata agatcgattt ggcgttttcg
      181 ttgaatccga cgccgaagcg agctctcgtc atcgatttca cgcatgcctt ttatctgcac
      241 ggctttggca tggttggggc gaagggcttt catgcgtcca actggggcga gctcaacaag
      301 cccgaagtca gaatcgccgt cgatatcggc tcggtgcacg agatcgcagc ccgccgcttc
      361 gcacccaatg cgacgatcat cggattgaag gcgcgcgacg attgcatcct ggctgtccaa
      421 tccggccgag ccgactgcgt gatcctcgcg gtcaatctcg ggctgaccgc ggtcaagaaa
      481 aatcccgcac tcggcgagtt tcagatgctc catcagccgc gtgtgcaatt accgacctgc
      541 atgggcgtgc gcatggaagc ggacaagcgc tggcgcgatt ttctcgacgc ctgggtcgat
      601 tacaaccgcg gcatccagca gattcgggaa tggctctacg gcggcctcgc gattaacggc
      661 gtcaagcccg aagacgtccc tgccgacgtc gaattctagc gtgccagcgg aacgtcggcg
      721 cggtgactgg cacgcgatac gcctgggact tctcgtttct gtggagctat cgcggcctga
      781 tcttgatcgg ccttggagtg acgatcgcct atacgatcgg gacaatcctc ctcggtctgg
      841 tggtcggcct cgtcaccggg ctgctgcggc tgtcgaagaa cgtgctgatc acagcgccgc
      901 tggtcgccta tgtcgaggtc ttccgctgta cgccgctgct ggtgcagatc gtctggttct
      961 attacgcact gccggtcgtc ctcggcatcg atatcccggc tcacgtcgcg gcgacgttgg
     1021 tattgtcgct ctacaccggc gctttctatg ccgagatcgt ccgaggcggg gtcaactcga
     1081 tcgagcgggg gcagtgggat gcggcacgtg cgatcggcat gcggcgcgcg caagttatgc
     1141 gctatgtcgt gctgccgcag gcgatcaagc ggatgatccc gccctttatg aaccagtcga
     1201 tcattcagct caaaaatacc tcgctggtgt cgacgatcgc cgtcgccgat ctgctgtatc
     1261 agggcacgat catcactgcg gcgacctatc ggccgctcga ggtctatacg atggttgcgg
     1321 tcatctattt tctcgtgctg ttcccgctga cattggcggc gcaacaggtc gagcacaggc
     1381 tcgcgcgcgc ggattagagc ggaccgacga ggtgccggag atgatgatcg agattgcgga
     1441 tctgtggaag agcttcggcg cgctgccggt attgaagggc gtctcactga aggtcgagcg
     1501 cggctcggtc gtcgccatca tcggcccctc cggctcgggc aaatcgaccc tgttgcgctg
     1561 catcaacctg ctcgaggaac ccgaccgcgg tcacatccgt gttggcgatc gcgagagggg
     1621 atttggcgcc ggcgtacgta ccaaaccaag cgatcgcgag ctctccgcct ttcgcgccgc
     1681 gaccggcatg gtcttccacc acttcaatct gttcccacac atgacagcgc tcggcaacgt
     1741 catgacagga ccgagtatcg tcaagggtat ggcgcgacac gaggcgacgg agatcgcccg
     1801 cgcgctgctc tctaaagtcg ggctcgccga caaggccgaa gcttatccct cgcatctctc
     1861 gggcggacag cggcaacgcg tcgcgatcgc tcgcgccctg gcgatgaacc ccgccgtcat
//
LOCUS       DATLJT010000048         1789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3907774,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000048 DATLJT010000000
VERSION     DATLJT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907774"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..>1789)
                     /locus_tag="VM910_00955"
     CDS             complement(299..>1789)
                     /locus_tag="VM910_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HVI61154.1"
                     /translation="IRDHLSGANMVFVTAGMGGGTGTGAAPVIAKTAREMGILTVGVV
                     TKPFHFEGQRRMRTAEAGIAELHKVVDTLLIIPNQNLFRVANEKTTFADAFAMADQVL
                     YSGVACITDLMVKEGLINLDFADVRAVMREMGKAMMGTGEASGEKRALTAAEAAIANP
                     LIDDSSMKGARGLLISITGGKDLTLFEVDEAATRIREEVDQDANIIVGATFDEALDGL
                     IRVSVVATGIEPEKFTRSITPPTVTSHSSPVASSGAPENRLADLTARLRADNARLADR
                     AARQDAQPAAQAAAPQRTTHERAALAAIAAAVAPEIPAASAPVTPASYGDVTVRPIAQ
                     KPSLFPDRDTARSEPEQPATPETFIPPAAERPTARAPRMPKFEELPMPAQAEIRQARG
                     ESEEEHPQKSRLSLLQRLANVGLGRRDEETESPIAARATGPAMPPLPDRKAKRTGSEQ
                     PEPVSEYARRPAPQGLDSHGRSAPVAPAPQGDDHLDIPAFLRRQTP"
BASE COUNT          280 a          576 c          599 g          334 t
ORIGIN      
        1 ccgattcgtg cttttaggta tccccaagtc gccgcaaaca taatcacagc cccaaagagc
       61 gccaactcac gcttttttac cgattgttac cctatctctg ccgtattggc gcccgtcctc
      121 aaccaaattg cgccgcgctg cagagcgctt ccaacgcggc cgtaatcttt ctaactacgc
      181 cattaaccat ttgggatcag tcgcttgccc gccgtgtgac gcgtcctgca gacctcaaaa
      241 taacggtccc ggctcacccg agccgggacc tttctcgttg ccgaggctgt aacaaacttc
      301 acggcgtctg ccggcgcagg aaggcgggga tatcaagatg gtcgtctccc tgtggcgccg
      361 gcgcaacagg tgctgaccga ccatggctgt ccaaaccttg tggcgcaggc cgtcttgcat
      421 actccgatac cggttcaggc tgctcggaac ccgtgcgctt ggccttgcga tcgggcaagg
      481 gcggcatggc gggaccagtg gcacgggccg caatcggcga ttcggtctcc tcatcgcggc
      541 ggccgaggcc gacattggcc aagcgctgca gcagcgagag ccgcgacttc tgcggatgct
      601 cctcctctga ttcgccgcgg gcctgacgaa tctcggcctg ggccggcatc ggcagctcct
      661 cgaatttcgg catccgcggc gctcgggccg tcgggcgttc cgctgccggc ggaatgaagg
      721 tctccggcgt ggccggctgc tcaggctcgc tgcgagcggt atcgcgatcg gggaagagag
      781 agggcttttg ggcgatcggc cgtaccgtga cgtcgccata ggaggccggc gtaaccggcg
      841 ccgacgccgc ggggatctcg ggcgccaccg ccgctgcgat agcggcgagc gccgcgcgct
      901 catgcgtcgt gcgctgcggc gccgcggcct gcgccgctgg ctgcgcgtcc tgcctggcgg
      961 cacggtcggc gagccgtgca ttgtcggcgc gcaaccgcgc cgtcagatcg gccagccggt
     1021 tctcgggcgc gccggaggaa gcaacggggc tgctatggct ggtcacggtc ggcggcgtga
     1081 tgctgcgggt aaacttttcg ggctcgatgc cggttgcaac cacggagacg cgaatcaagc
     1141 cgtcgagcgc ctcatcgaat gtcgcgccga cgatgatatt ggcgtcctgg tcgacctctt
     1201 cgcggatgcg ggtcgcggct tcgtcgacct cgaacagtgt caggtccttg ccgccggtga
     1261 tggagatcag aagaccgcgt gcgcccttca tcgaggaatc gtcgatcagc ggattggcga
     1321 ttgcggcctc ggctgcggtc agcgcgcgtt tctcgccgga cgcttcgccg gtacccatca
     1381 tcgccttgcc catctcgcgc atcacggcgc gaacgtcggc gaagtcgaga ttgatcaggc
     1441 cttccttgac catcaggtcg gtgatgcagg cgacgcccga atagagcacc tggtcggcca
     1501 tcgcgaaggc atcggcgaag gtggtctttt cgttggcgac gcggaacagg ttctggttcg
     1561 ggatgatcag gagcgtgtcg acgaccttgt gcagctcggc gatgccggcc tccgcggtgc
     1621 gcatgcgccg ctggccttcg aaatgaaaag gcttggtcac cacgccgacg gtgaggatgc
     1681 ccatctcacg ggcggttttc gcgatcacgg gagccgcgcc ggtgccggtg ccgccgccca
     1741 tgccggcggt gacgaacacc atgttggcgc cggagagatg gtcgcggat
//
LOCUS       DATLJT010000049         1910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1150838,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000049 DATLJT010000000
VERSION     DATLJT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150838"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VM910_00960"
     CDS             complement(<1..331)
                     /locus_tag="VM910_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61155.1"
                     /translation="MIEGAKQHQAPPPTFRLEHVNSADANNQFFSITMFDDARAAMSC
                     WHGEVDTFAADGNSAEPMSILHTMLLAGIGLHGYGLEWRQALQVRDQLVSLAKASDRQ
                     VSEVHVAG"
     gene            complement(572..907)
                     /locus_tag="VM910_00965"
     CDS             complement(572..907)
                     /locus_tag="VM910_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61156.1"
                     /translation="MRESRFTHLTRLKRPLLIGAAVWVATQHPAHAADEIQVYNAAIA
                     AVGQFTVQQHLNYTPLGVKEPPFPGGLVSNGAINGTPEFAYGLTDWWEVGLYLPFAIQ
                     NQTIVSDNP"
     gene            complement(922..1893)
                     /locus_tag="VM910_00970"
     CDS             complement(922..1893)
                     /locus_tag="VM910_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HVI61157.1"
                     /translation="MKNFADLTEREVLAVAISSEEEDSRIYMTFAEDLRERYPDTAKI
                     FEEMAEEERGHRHRLLEMYEQRFGAHLPPIRREDVKGFLRRRPIWLTKNLPLDTIRKE
                     VETMELQAERFYAKAAERAEDVGVRRLLGDLAEEEKSHENLAVKLTGEILKPDVREAE
                     DRTRRRMFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHQNWQTFLVGLAASIGAGIS
                     MGFAEALSDDGSLTGRGSPWLRGLTCGAMTAIGGLGHTLPYLVPDSLPNAFWIATGIA
                     SVVVFFELWAIAYVRARYMDTPFLQAVFQIVLGGVIVLGVGILIGGA"
BASE COUNT          389 a          611 c          557 g          353 t
ORIGIN      
        1 cgccagcgac atggacctca gacacctgtc tgtcggatgc cttcgccaaa ctaacgagct
       61 gatctcgcac ttggagggcc tgacgccact ccagcccata gccgtgaagg ccgatcccag
      121 ccagcaacat ggtatgcagg atcgacatcg gctcggcgct gtttccgtcg gctgcgaacg
      181 tatcgacctc gccatgccaa cacgacatcg ccgcacgagc atcgtcgaac atcgtgatgc
      241 tgaaaaattg gttattcgcg tctgcggagt tgacgtgttc caaccggaat gtcggaggtg
      301 gcgcctggtg ctgcttggcg ccctcaatca tcgcggcctc gaactcgtcg caggtcgctg
      361 ccgcccgagc cgcggtaggc gacaacgcca acagcgcgct gatcactaat atccgaatac
      421 gcatgaaaca cctcacacta gctacgctcg ttcttggacc caagtctgga cccaaactca
      481 ataggacggg cacgaaccaa taaaacagaa gcacactctg catcggcgca aggtcttgaa
      541 atctctcact ctcgggtact aggcgggatg ctcaaggatt atcgctaacg atggtctggt
      601 tctggattgc gaacggcaga tagagcccga cctcccacca gtcggtcaag ccataggcaa
      661 attccggcgt gccgttgatg gcgccattgg agaccagccc gccggggaac ggcggctcct
      721 tcaccccgag cggggtatag ttgagatgct gctgaacggt aaactggccg accgcggcaa
      781 ttgcggcatt atagacctgg atctcgtcgg ccgcgtgtgc cggatgttgc gtcgcgaccc
      841 aaaccgccgc gccgatcagc agcggacgct tgagccgcgt gagatgagtg aatcgacttt
      901 ccctcatcgc cttgtgccgc gctacgctcc tccgatcaga atcccgacgc caagcacaat
      961 aacgccgccg agcacgatct ggaacaccgc ctgaaggaac ggcgtatcca tgtaacgcgc
     1021 gcggacatag gcaatcgccc acaattcgaa gaacacgacg acgctcgcga tcccggtcgc
     1081 aatccagaag gcgttgggca gggagtccgg aacgagatag ggcagcgtat ggccgaggcc
     1141 tccaattgcc gtcatcgcac cgcaggtcag gccgcgcagc cacggcgatc cgcggcccgt
     1201 gagcgaaccg tcgtcggata aagcctccgc aaagcccatg ctgatgccgg cgccaatcga
     1261 ggcggcgagg ccgaccagga aggtttgcca gttttgatgg gtcgcgaacg ccgccgcaaa
     1321 caatggcgcc agtgtcgaca ccgagccgtc catcagtccg gcgagccccg gctgcacata
     1381 ttgcaaaacg aacatgcgcc gccgggtgcg atcctcggcc tcgcgcacat cgggtttcaa
     1441 tatctcgccg gtgagcttga cggcgagatt ctcgtggctc ttttcctcct cggcgagatc
     1501 gccgagcagg cgacgcacgc cgacatcctc ggccctttcg gcggcctttg cgtagaaccg
     1561 ctcggcttgc agctccatgg tctcgacttc cttgcggatg gtgtcgagcg gcaggttctt
     1621 ggtcagccag atcggacgcc gtcgcaaaaa tcccttgacg tcttcgcggc ggatcggcgg
     1681 cagatgcgca ccgaagcgtt gctcgtacat ttcgagcagg cgatggcggt ggccgcgctc
     1741 ttcttcggcc atctcctcga aaatcttcgc cgtgtccggg tagcgttccc tgaggtcctc
     1801 ggcaaaggtc atgtagatgc gactgtcttc ctcctcggag gagatcgcca ccgccagcac
     1861 ctcgcgctcg gtcagatcgg cgaaattctt catcgaagcc ttcctgagag
//
LOCUS       DATLJT010000050         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2759122,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000050 DATLJT010000000
VERSION     DATLJT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759122"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VM910_00975"
     CDS             <1..990
                     /locus_tag="VM910_00975"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVI61158.1"
                     /translation="REMLNILDERKFPADEVVALASRRSVGIEVSYGDRTLKVKALEH
                     YDFSDVDICLMSAGGAVSREWSPKIAAQGAVVIDNSSAWRMDSDVPLIVPEVNADAAA
                     GFVKKNIIANPNCSTAQLVVALKPLHDKAVIKRVVVATYQSVSGAGKDAMDELFSQTK
                     AVYTNDELVNKKFPKRIAFNVIPEIDVFMEDGYTKEEWKMMAETKKILDPKIKLSATC
                     VRVPVFVGHSEAVNIEFEKPITPDEARDVLRNAPGCLVIDKQEPGGYVTPYEAAGEDA
                     TYISRIREDATVENGLALWCVSDNLRKGAALNAVQIAEVLINRKLLSAKKKAA"
     gene            1013..1561
                     /locus_tag="VM910_00980"
     CDS             1013..1561
                     /locus_tag="VM910_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HVI61159.1"
                     /translation="MTDQPALPRSTPVRIRRGFEGVLFNSRWLMAPFYVGLMVALAVL
                     FLKFLRMLWEFMLHAPGAKSTETILEALSLIDVTLVGNLILIVVFSGYENFVSRIETS
                     GNPNWPLWITKIDFAGLKQKLLASIVAISAIHVLEAFLNIDASFDATRMTWLVAVHLV
                     FVVSTLLLALSDRWTPDRANGG"
     gene            complement(1631..2284)
                     /locus_tag="VM910_00985"
     CDS             complement(1631..2284)
                     /locus_tag="VM910_00985"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI61160.1"
                     /translation="MITFPRQLINGYRAFQTQRLPTEQSRYRELSERGQSPEVMVIGC
                     CDSRVSPEVIFDARPGELFVVRNIANLVPVYAPDGGTHGVSAALEYAVQVLRVKHIVV
                     LGHAQCGGVRAFIDKIEPLSPGDFIGKWMSMFVKPGEVVEQRPQETIQDFATRIEKAS
                     VFRSLENLTTFPCIQILVDRGKLQLHGAYFGVAEGSLFVLDKATREFRSVRESQPSP"
     gene            2384..>2983
                     /locus_tag="VM910_00990"
     CDS             2384..>2983
                     /locus_tag="VM910_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVI61161.1"
                     /translation="MIRPRRSLLFMPGSNARALEKARSLPADGLILDLEDSVAPDAKA
                     KARDQIAAAIAAKGFGKREILIRTNSLDTPFWPDDIAMAAKAKPDGILVPKISTVDDL
                     KLVGGRLNEAGGGSSIEVWAMIETARAVLDADRLAAASRDLSTRLVGFVFGPNDISRE
                     TRIKMLPGRAAMIPMITHCILAARAHGLEILDGPYGDIGN"
BASE COUNT          593 a          918 c          903 g          569 t
ORIGIN      
        1 cgggagatgc tcaatattct cgacgagcgc aaattccccg ccgacgaggt tgtggcgctg
       61 gcgtcacgcc gcagcgtcgg tatcgaagtc tcctatggcg accgcaccct gaaggtcaaa
      121 gcactcgagc attacgactt ctccgacgtc gacatctgcc tgatgtcggc cggcggtgca
      181 gtttccaggg agtggtcacc gaagatcgcc gcgcaaggcg cggtcgtgat cgacaattct
      241 tccgcctggc gcatggattc ggacgtgccg ctgatcgtgc ccgaggtcaa tgcggacgcg
      301 gccgcgggct tcgtcaagaa gaacattatc gccaacccga actgctcgac cgcgcaactc
      361 gtggtcgcgc tgaagccgct gcacgacaag gcagtaatca agcgcgtggt ggttgcgacc
      421 tatcaatcgg tttcaggcgc cggcaaggat gcaatggacg agttgttctc gcagaccaag
      481 gccgtctaca ccaacgacga gctggtcaac aagaaatttc cgaagcgaat cgccttcaac
      541 gtcatccccg agatcgatgt gttcatggag gacggctaca ccaaggaaga atggaagatg
      601 atggcggaga ccaagaagat tcttgatccc aagatcaagc tctccgccac ttgcgtgcgc
      661 gtgccggtct tcgtcggcca ttcggaagcg gtcaacatcg agtttgagaa gccgatcacc
      721 ccggacgagg cgcgcgacgt cctgcgcaat gcgccgggat gtctcgtgat cgacaagcag
      781 gagcccggag gctacgtcac gccttacgaa gcggccggcg aggacgcgac ctatatcagc
      841 cgcatccgcg aagatgccac cgtcgaaaac gggcttgctc tgtggtgcgt atcggacaat
      901 ttgcgcaagg gtgcggcgct gaacgcagtc cagatcgccg aggttctgat caaccgcaag
      961 ctgctcagcg ccaagaagaa ggcggcgtaa gcccgacgca ggagccggag acatgaccga
     1021 ccagccggct cttccccggt cgacccccgt acgtatccgg cgcggcttcg aaggcgtgct
     1081 gttcaacagc cgctggctga tggcgccgtt ctatgtgggc ctcatggtcg cgctcgccgt
     1141 gttgtttctc aagtttctgc gcatgttgtg ggagttcatg ctgcatgcgc ccggtgcgaa
     1201 atcgaccgaa accattcttg aagccttgtc cctgatcgac gtgacgctgg taggcaatct
     1261 cattctcatc gtggtgtttt cgggctacga gaatttcgtc tcccgcatcg agacgtcagg
     1321 caatccgaat tggcccttgt ggatcaccaa gatcgatttc gccggactga aacagaagct
     1381 gctcgcctcg atcgtggcga tttcggcgat ccacgtgctt gaggcgtttt tgaacatcga
     1441 tgcatctttc gacgcgacca ggatgacctg gctcgtcgcc gtgcacctgg tcttcgtggt
     1501 ctcaacgttg ttgcttgcgc tgtccgatcg ctggacgcca gacagagcga atggcgggta
     1561 ggcggtagag cgcgatagcc ttatgccaaa ccgccatcgc gttccccgct tgtcgcgagg
     1621 cgccttctcg tcagggtgag ggctgcgact ccctcacgct cctgaattcc ctggtcgcct
     1681 tatcgagcac aaacagcgag ccctccgcca cgccgaaata ggcgccgtgc aattgcagct
     1741 tgccgcggtc gaccagtatc tggatacagg gaaaggtcgt cagattttcc aggctgcgga
     1801 acaccgaggc cttttcgatc cgggtcgcga aatcctgtat ggtttcctgc gggcgctgtt
     1861 caacgacctc gcccggcttg acgaacatcg acatccactt gccgatgaaa tcgccgggcg
     1921 acaacggttc gatcttgtca atgaaggcgc gaacgccgcc gcattgcgca tgcccgagca
     1981 ccacgatgtg ctttacgcgc aagacctgca cggcatattc cagcgccgcc gagacgccgt
     2041 gcgtgccgcc gtccggtgcg tagaccggca cgagattggc gatgttgcgg accacgaaca
     2101 gttcgcccgg cctggcgtcg aagatcactt cgggcgagac gcgggagtcg cagcagccga
     2161 tcaccataac ctccggcgac tggccccgct ccgacaattc gcgatagcga gactgctcgg
     2221 tcggaagccg ctgggtctga aacgcccggt acccgttgat cagttgtcgc gggaaggtaa
     2281 tcattgcctt tgctaaccat aagccgatga cgggaacaag acttctcccg cgcgaagcag
     2341 gatgttatcg cctgcgccag aacatgtcag agggaacaag ccgatgatcc gtccgcgccg
     2401 cagtctgctg ttcatgcccg ggtcgaacgc ccgcgcgctc gaaaaagcgc gcagcttgcc
     2461 agccgacggc ctgatccttg acctcgagga ttccgtcgcc cccgacgcca aggccaaagc
     2521 gcgcgatcag atcgctgcgg caatcgctgc caaaggattc ggcaagcggg aaatcctgat
     2581 ccgcaccaat agcctcgata cgccgttctg gcccgacgac atcgcgatgg ccgcgaaagc
     2641 gaagcccgac ggcattctgg tgcccaagat ctcgaccgtg gatgacctca agctggtcgg
     2701 cggtcgcttg aacgaggccg gcgggggttc ctcgatcgag gtctgggcca tgatcgagac
     2761 ggcgcgtgcg gtgctcgatg ccgacaggct tgcggcggcg tcgcgcgatt tgagcacaag
     2821 actcgttgga ttcgtattcg ggcccaacga tatttcgcgc gagacgcgga tcaagatgct
     2881 gcctggccgt gcggccatga tcccgatgat cacgcattgc atcctggccg ctcgcgcgca
     2941 cggcctcgaa atcctcgacg gcccctacgg cgacatcggc aat
//
LOCUS       DATLJT010000051         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2069975,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000051 DATLJT010000000
VERSION     DATLJT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069975"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            809..946
                     /locus_tag="VM910_00995"
     CDS             809..946
                     /locus_tag="VM910_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61162.1"
                     /translation="MSQRSPVINAIVDALAELGVRHIEMPATPERVWRAIREANGRRQ
                     V"
     gene            1318..2037
                     /locus_tag="VM910_01000"
     CDS             1318..2037
                     /locus_tag="VM910_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI61163.1"
                     /translation="MRAVDVMTTNVITVTPDTSIQALAALLSERGISGVPVVDAAGRL
                     VGIVSEGDLLHRAETGTARRTERRRFRWLDTVASDQELARDYMKSRGRVVSDVMSREV
                     IAVTEMTELAEIADLLEIKRIKRVPVVRDGKLVGIVSRANLVRALATVKAESSSSPDI
                     GDHTIRQKLFAELQAQEWAGPWAADIVVRDGIVHLWFPEGHSAEEKRAVHIAAENIAG
                     VRAVEEHIVQIPLIQAIMTRL"
BASE COUNT          437 a          646 c          694 g          415 t
ORIGIN      
        1 tcaggcacac gagagtcgta atggccgagt gcaatcggag gagttagtct ctacggtttt
       61 gccgccgtca ggacgccggg gccgcggcaa cggccgtagg cgtatgagta tcagcgccca
      121 agttcgtcga ccggtgtgaa ggtctcttaa gggtcgattt gagacactct ccggctgtca
      181 gcactgtttc gacctatact ttacgtaaat ttcgccggct gatctaatat tccgatcgtc
      241 aggaggaagc gctttaagta tccttccagc gtaaacgccc gcctggcgag cgacagctgc
      301 gctgtgaatt ccatagctga tcaggccacc gattgcggtg aacattcgcc cctcatagat
      361 cgccggtacg gcatagcgtg atgctagccc cacgagctgc ttgcgcctgt agataaagaa
      421 cgaatcggcg ttaggggggc gccgcaaagg agccggcagg ccgcacactc gcgcgatgct
      481 tcccgagctc gaccgctaac cgtgacctcg aggtcgagga tgtccttcct tcggtgttca
      541 tgacgcaggt aatgaacgac gaaacagccc ctatccaggt gcgcctcgat gctgcatcgg
      601 ccccgacttg tccggtatgg aaaatttttc cacatcggcc tccttgaaaa ccttttgcgg
      661 gcgatcgagc aaccggcgag ccggggccgc gggcggccgc caagggcagc cgcgactaag
      721 tcggcggccc gcaaacccag cgtgccgagc taggtgagcg cgtcctggcc ctggctaccg
      781 gcaggacccg acaggaactt tacctggcgt gtcccagcga tcgcccgtga tcaacgcgat
      841 cgtcgatgcg cttgccgaac tcggggtacg ccacatcgag atgccggcga cgccggagcg
      901 ggtctggcgc gccattcgag aggcgaatgg gcggaggcaa gtctaatttc tcggcccgcc
      961 ccaaatcgac gacgcgaccc ctggaataaa ggacagctga tgccccaaag cggccgtgct
     1021 atggtcgtgt ccgccgggcg gtgtaggagc ggtgggtaag tagcccgccg cattgatcac
     1081 ctcttggttg cggcaaagcc gacttttcgt tgcccgagcc ggtcagaagt gcgtttcttg
     1141 cccgaaacgg ccgggaatct ggctaattgt cggatcagag caactgagcc actggccgag
     1201 tctgcttcgc gccgaggcgc cgtgatgcca gtcccgcttg acttgcgtca atgtgggcag
     1261 gagaccgccc cgatacaaag ccgatggaaa gaggtgcgtt agacggaggg ccttctgatg
     1321 cgggctgtgg acgtgatgac gacaaacgtg attaccgtca ccccagatac atcgattcaa
     1381 gcactggcag cgctcctctc cgaacggggc atcagcgggg ttccggtcgt cgatgcggca
     1441 ggccggctgg tcggcatcgt cagcgaaggt gacctcttgc accgcgccga gacagggaca
     1501 gcgcgccgaa cagagcggcg acgcttccgg tggctcgata ccgtcgcatc tgatcaagag
     1561 cttgcgcgcg attacatgaa gtcccgcggc cgagtcgtca gcgacgtcat gtcgcgcgag
     1621 gtcattgcgg tcaccgagat gaccgagctc gctgaaatcg ccgacctctt agagatcaaa
     1681 cggatcaagc gggtgcccgt ggtccgcgat ggcaagctgg tcggcatcgt cagccgggcg
     1741 aacctcgttc gggcgttggc gacggtcaag gccgagtcct cgtcgagccc ggatatcggc
     1801 gatcacacaa tccgccaaaa gctcttcgcc gagctgcaag cgcaggagtg ggccgggcct
     1861 tgggccgcgg acatcgtcgt tagagacggg attgtgcatc tctggttccc agaaggtcac
     1921 tctgcagagg agaaacgggc ggtgcacatc gccgccgaaa atattgcagg tgtccgtgca
     1981 gtcgaagagc acatcgtgca aattccgctg atacaggcga taatgacgcg gctgtaacct
     2041 ctctttcttt tctcaagtct aagtgactgt ccgcgaacat gttgattctg tggagtcggt
     2101 tgtgattcaa gagaggcggc cggaatcggc gctgacgcgc cgctcctccg gcgaccgacg
     2161 aatgcgaagg gtatgcccct caacggccat tg
//
LOCUS       DATLJT010000052         1840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_921163,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000052 DATLJT010000000
VERSION     DATLJT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921163"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VM910_01005"
     CDS             <1..209
                     /locus_tag="VM910_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61164.1"
                     /translation="AIVEMTSVATEFTETELTAAMRRLIKTGAIRNVEEGPPTRRRSF
                     LETAELPGLDGASSSNVVNLRAAE"
     gene            complement(1113..1379)
                     /locus_tag="VM910_01010"
                     /pseudo
     CDS             complement(1113..1379)
                     /locus_tag="VM910_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022538.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1645..>1840)
                     /locus_tag="VM910_01015"
     CDS             complement(1645..>1840)
                     /locus_tag="VM910_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61165.1"
                     /translation="VRQLDLGHILWCGRVVRVERAHRVRVVRSVQKMPDVGNGAVAIG
                     VGIAARNSQGALIAVLDVVG"
BASE COUNT          352 a          590 c          555 g          343 t
ORIGIN      
        1 gagccatcgt cgagatgaca tcggtcgcga ccgagttcac agaaaccgag ctcacggccg
       61 caatgcgtcg gctcatcaag acgggggcaa tccggaacgt cgaggaaggt ccgccgacgc
      121 ggcgtcgctc tttcctcgaa accgcggagc tgccgggcct cgacggggct agtagctcca
      181 acgtcgtcaa tctgagggcg gctgagtgaa tcgcgtctgc aaccgtggct gcaccgtacg
      241 gggtggtctg tcgagagcgc gacgtcgccg ggaaccacgt cttcgtctgt aaggtggcga
      301 tctcgtgttg ccccagcgtt ttgcgcacga tgtcgagccc actcgagagc ggcgcatagc
      361 ggaagctgcg cccgccgtct ccggtgcgga tggtgcgggg tcccggggtg cggctgcgca
      421 aggcgcatca acacaaataa tatatcacgg ggccgctttg gctgtcgtgg gccgccccat
      481 cgcaaattat ggctcaaagt aggaacttta ccagacggca tcagccatgg atgcggccaa
      541 cttggcacga tgacgccgtc ctcgcgcgca tgtcggatga agagctgata aaggcgctcg
      601 aagagaaggg ccaatcggct cgcgttaagc tcaatctgaa ttatggcgac gggcggctga
      661 aaaacgcttt tcgcgcgtct tgaagttcaa ttcggcgcga agggttgccg acgggtgggc
      721 ccccatcttt gccgccgagg cgggttcccc ttgctaactg ccccgctgac cgttgcaaag
      781 tgtgcgcatt acccgctgtg cggctcgatc gaagccgtgc cggcaagggg cgggggcgtg
      841 cgcccatctc atcttccgag cgcgttccgg ttgcgcgagc cacggttgcc gacctcgccc
      901 tatggatcct cgaagctgat gcctgagatc atgctgcgcg gcgccggtag caccctcgct
      961 tagcgccacg taatcttgcg ttacttcacc cacctcgtca cggtcgaagc cgcgctcggc
     1021 ctgcggcaaa ggtggaggtc ttcgccaccg actacccgaa caccggcgcg aagaatgacg
     1081 agatcagccg ttgatcaccg cagaagtact aagtaccggc tctccaccca gcgatcgcac
     1141 atagcctcgt cggaaaggtc gtaagtgtgc ttgaggatcg ctagccccgc catcagccgc
     1201 gtcggcaatg gcggctggcc cggcttgtcg gtgtagaccg ctccaaacgt ctgttccaga
     1261 aaaccccaat cgatcgcccg cgccagcttc accaacgcgt gtttcacatc gatgatctgg
     1321 tcaagccgtg agcggaacag atcctgctca ccggtctccc gtcgctcgcg tggttgcatc
     1381 gcaaatcgcc cgctccactt ctcgtccgct ctcagcggcg cgtcctatac cccgccatgt
     1441 tgcgtatgcg tactctaccg gccgggttca tcgagccgtg ccttcctaca aaggccgaca
     1501 cgctgccttc tggcgggctg tggattcatg aaatcaagca cgacgggttc cgcatcatcg
     1561 cctgtaagaa tggcgcgcag gtgaggctca tcagccgtcc gaagagctgc ggatcggctt
     1621 gggcggttgc gacagggccg gtactcaacc gacaacgtca agcacagcga ttagcgcacc
     1681 ttgtgaattt cgcgctgcaa ttccaacacc aatcgcgacg gcaccgttgc cgacatccgg
     1741 catcttctgg acactgcgca cgacgcgaac gcggtgggcg cgctcgaccc gaactacacg
     1801 accgcaccac aagatatgac caaggtccag ttgccgcacc
//
LOCUS       DATLJT010000053        12734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_231754,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000053 DATLJT010000000
VERSION     DATLJT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231754"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VM910_01020"
     CDS             <1..656
                     /locus_tag="VM910_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HVI61166.1"
                     /translation="NTAGGMAGGDHFDLDIAVGPDAKLTVTTAAAEKIYRSPGPDTEI
                     RVKLDIGSGGTLGWLPQETILFDQIRLRRSIEADLAPDASLLLAEGIVFGRAAMGETL
                     AHGHLLDRWRVRRAGVLVFAESLRLDGAIADRLARRAVAGGAVAIASVLKVPGDEAAV
                     AAVRAKEKDFAGEVGVSTWNGLALARLVAPDGAALHRDLIAVLTAFGNMPLPRLWVN"
     gene            741..1043
                     /locus_tag="VM910_01025"
     CDS             741..1043
                     /locus_tag="VM910_01025"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HVI61167.1"
                     /translation="MNLTPREKDKLLIAMAAMVARRRLERGVKLNHPEAVALISDFIL
                     EGARDGRTVAELMEKGAHVISRTQVMDGIAEMIPEIQVEATFPDGTKLVTVHEPIR"
     gene            1180..1509
                     /locus_tag="VM910_01030"
     CDS             1180..1509
                     /locus_tag="VM910_01030"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HVI61168.1"
                     /translation="MIPGEYFIQDGEIELNKGRKGLTLTVSNTGDRPIQVGSHYHFFE
                     TNPALRFDRKKARGMRLDIPAGTAVRFEPGQTRDVNLVALAGTRTVYGFRQDVMGKLE
                     KGKSQKS"
     gene            1821..2891
                     /locus_tag="VM910_01035"
     CDS             1821..2891
                     /locus_tag="VM910_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI61169.1"
                     /translation="MEIGLVLQGDGALGAYEWGAITALFDVIDEAIHNGQDITLRVVT
                     GVSIGAINAACVVGAADRADARNRLSAMWSDFMIDLPYLPPSANRDLALYNVPHFYTY
                     RSDLLAAPTWTYLYDTHQLLQTLSDHVDFNALNANKTAFVITAVDVESGVLTRFTNQK
                     VGDTDPTAIEPHHVLASGSIPPQFPWTDISASDNTVHHYWDGGIVDNTPLGDAIDAFS
                     SDPGVTRLLVIMNLFPQTASLPRSLTQVNERLDQLRFGNRLRQDSSTARQMNALIATI
                     QDLSALLSGPLPEDLQKRVDKANAYKLVKLIEISLTADAVSDDPNSFRDFSREGIVAR
                     RDAGRRIALSALRTTFQSKLAA"
     gene            2905..4614
                     /gene="ureC"
                     /locus_tag="VM910_01040"
     CDS             2905..4614
                     /gene="ureC"
                     /locus_tag="VM910_01040"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HVI61170.1"
                     /translation="MPSKISRSVYADMFGPTTGDRVRLADTDLIIEIEKDFCTYGEEV
                     KFGGGKVIRDGMGQSQLTNKQGAVDTVITNALIVDHWGIVKADVGIKDGNIYAVGKAG
                     NPDIQPGVTIVIGPGTEVIAGEGKILTAGGFDTHIHFICPQQVDEALMSGVTSMLGGG
                     TGPAHGTFATTCTPGPWHIARMIQAADDFPVNLGFAGKGNASRPAALEEMIKAGACAL
                     KLHEDWGTTPAAIDCCLSVADDYDIQVMIHTDTLNESGFVEDTIAAFEGRAVHSFHTE
                     GSGGGHAPDIIKIASLPNVLPSSTNPTLPYGINSQAELYDMIMVCHHLSPDIPSDVAF
                     TESRIRAETIAAENVLQDMGVISMFSSDSQAMGRIGECWLRCIQTADAMKTGRGKLPE
                     DAPGNDNFRVRRYVAKITINPAITHGIADVLGSVEVGKIADLVLWEPAFFGAKPKIVI
                     KNGFISWAVMGDPNASLPTPQPTYYRPMFGAYGDALAASCITFVSGAAHAAGVKERLG
                     LRRQVMPVRNTRKIGKRDMVCNAATPKIEVSPETFAVTVDGKHATVPPLKTVSLNQKY
                     FFS"
     gene            4625..5317
                     /gene="ureE"
                     /locus_tag="VM910_01045"
     CDS             4625..5317
                     /gene="ureE"
                     /locus_tag="VM910_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935999.1"
                     /note="involved in the assembly of the urease
                     metallocenter; possible nickel donor; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HVI61171.1"
                     /translation="MVVIETVLGNISDPEWAGRLAASEVDVLEIDQWEAQKSRFRKTT
                     AKGAEVAVSVDRGTYIRDGDVLLWDAQALSALVARIEVRDVMIVHLDELMHLAPQVAM
                     RTCVELGHAMGNQHWPALVKGDLVYVPLTVDRKVMASVMETHHFEGVRYEFVSGREVV
                     PYLAPHESRRLFGAAEGPVHSHTHESYATTDTETGRVYGRPPVRVHQHPETDAHAHGS
                     PPKAGFTHDQGG"
     gene            5379..6020
                     /locus_tag="VM910_01050"
     CDS             5379..6020
                     /locus_tag="VM910_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HVI61172.1"
                     /translation="MFPIGGFSFSCGLESAIQKGVVADAITLRAFTRTAVEQAARGDG
                     IALIAAHRAATVGDVDALIRIDAQVYARKLSDEARLMSVRMGKKFTEMGVEVIGAPLL
                     GTWRKCIAMSVTPGCYPVALAISFTVQNLSARQAFVVHQNGIAATILGAALRLMKISH
                     VETQKILYELNGLADAAYENVAAARLSDMAGYAPLTEILAAVHNKAHVRLFMS"
     gene            6041..6685
                     /gene="ureG"
                     /locus_tag="VM910_01055"
     CDS             6041..6685
                     /gene="ureG"
                     /locus_tag="VM910_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HVI61173.1"
                     /translation="MSKKITRVGIGGPVGSGKTAIVEAITPRLLDLGIKVLIITNDVV
                     TTEDAKHVQRTLKGVLLQERILGVETGACPHTAVREDPSMNLAAVEDMERRFPDSDVV
                     LIESGGDNLTLTFSPALVDYFIYVIDVAAGEKIPRKNGPGITHSDILVINKTDLAPHV
                     GASLEVMKQDSRLMRGSKPFLFTNCKTGEGIAELVDLIKREFLFDINPRVQRSA"
     gene            6682..7632
                     /locus_tag="VM910_01060"
     CDS             6682..7632
                     /locus_tag="VM910_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HVI61174.1"
                     /translation="MIPAASTPWAHAEELGASVPELAAFQDEPLQMKSGTVGKTGFLR
                     LGFEHRANKTILANLERRAPYMVQRALYCDPEMPDLACVFLITTTGCLLQGDRLALDI
                     TLGPRAEAHVTTQSATKIHAMDANYAAQTQIITLADDAYLEFLPDPVIPHRQARFVSD
                     TQISIAPSATLLFSEIIQPGRKHHRPDECFGATVISISTSAARPDGCRLFTEKLLIEP
                     QRYAMRQTGVMDSFDVFANVILCTPKDKADRVYERVEADVDLADGVAFGACRLPNDAG
                     LIYKILGRETAQVKAKVREFWGVVRREVTGAGLPPPFLWR"
     gene            7694..8710
                     /gene="yut"
                     /locus_tag="VM910_01065"
     CDS             7694..8710
                     /gene="yut"
                     /locus_tag="VM910_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HVI61175.1"
                     /translation="MEKFLAKWQSLCASSRVLRFVDINLRGIGQVMFQDNPLTGALFL
                     IAIAWGSYAAGVPQVAIAGVVAVIVATLTAQWLRVDDESLNAGLYGFNAILVGLALAT
                     FLAPGPLLWVYVILGATVSVVVMLATTNVVKPWGGALTFPFVLTTWILLLATYGFSGL
                     AGTALPAGKVVTAFQPYQASPLQLIDLVQGVFQSISQVFLKASGIAALLLLAGLAVNS
                     LAAAAFALGGAILAVLTAHLFGAESDLITGGLLGFSPVLTAIALGTVFYQPSWRVAVY
                     TALATVFTVIAQSALNVALTPFAIPALTAPFVLVTWLFLLPRQRLEEVPAVAPDIEKP
                     AAAA"
     gene            8931..10376
                     /locus_tag="VM910_01070"
     CDS             8931..10376
                     /locus_tag="VM910_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HVI61176.1"
                     /translation="MGLIGILLGLALLMWLAYRGWSILLVSPLAALVAAVFSGEPLLA
                     HWTQTFMSGAARFVAQWFPLFLLGGLFGKLMGDSGSVSAIAQYLTKKLGASRTMLAVV
                     AASAIVTYGGVSVFVAFFVLAPLAHEMFRAANLPRRLIPAAVGLGAFTFTMSVMPGTP
                     SVNNAIPMPYFGTTTFAAPGLSIIASAIIFVFGMWWLGRAEAAARKAGEGYAGEAGTA
                     PVVNEMVREQAAPAGDFDPAELPHGKQAESGPSFVLAMLPILVVIVTNFLMSLVVFPR
                     LDFSFLSEHQWGGITIGAVSGVWSVLVALAAANLTVILINFRRLPSPRESLDAGANSA
                     ALPMLMIASLVGFGAVVAALPAFTLVRDAVFSVQGGPLVSLALSMNVLAGLTGTASGG
                     MAIVLNAFGADFMRLAIEHRIDPDLMHRITTMSAGTLDALPHNGTVLLLLQISGLTHR
                     ESYLDMVMTVIVSCIIALVAVLILGSVFGSF"
     gene            complement(10439..11182)
                     /locus_tag="VM910_01075"
     CDS             complement(10439..11182)
                     /locus_tag="VM910_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61177.1"
                     /translation="MTPEQIAYDYDEQPESEDEQKYREDVGYKIAESEAFCEEEHCDS
                     PCSLDRPVAGVASGLRSFCCLRYASDPCGRNSCTAPLTRQRTHPFACRVAARGTRRKL
                     SRTAHGESATCRFDMRGPRLQLVHAIDLPVDAFQRSGEHLFALQGMFGCAWKAFASPR
                     PFAACFAALFARQSPFLVAHIAQPLAQRPEVIKPGIIDFGMVTAQDDLMLIVAENVAF
                     EFAGYGHDYPRLFLTLHSSPIPTRSEVSA"
     gene            11213..11443
                     /locus_tag="VM910_01080"
     CDS             11213..11443
                     /locus_tag="VM910_01080"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HVI61178.1"
                     /translation="MIALIVFPYLGIFAYLITQSRGMTERSSQQAQQAREELRRVAGF
                     SVADEIEKLDRLKKSGTITDQEFTRLRAKLVA"
BASE COUNT         2494 a         3839 c         3916 g         2485 t
ORIGIN      
        1 tcaataccgc cggcggcatg gcgggcggcg atcatttcga tctggatatt gcggttggcc
       61 ccgacgcgaa gcttacggtc accacggccg ccgccgaaaa aatctaccgg tcgcctgggc
      121 cggacacaga aattcgcgtg aagctcgata tcggttccgg cggtacgctc ggctggctgc
      181 cgcaggagac gatcctcttc gaccaaatcc ggctgcgccg ctcgatcgaa gcggacttgg
      241 ccccggatgc aagccttctc ctggccgaag gcatcgtctt cggccgcgcg gccatgggcg
      301 aaacgctggc gcacggccat cttctcgatc gttggcgggt gcggcgtgcc ggcgttctcg
      361 tctttgccga aagtctccgg ctggacggtg cgatcgcgga tcggctcgcc cggcgcgcgg
      421 tcgccggcgg cgcggtcgcg atcgcaagcg tgctcaaggt cccgggcgat gaagcggcgg
      481 ttgcggccgt gcgggctaag gagaaagatt tcgccggcga agtcggcgtt tcaacctgga
      541 acggccttgc gcttgcgcgg ctcgttgcgc ccgacggcgc cgcgttgcac cgcgacttga
      601 ttgccgtttt gaccgctttc ggcaatatgc cactgccgcg actttgggtg aactagagca
      661 tgatctcgaa aagtggaaac cggttttcgg aaaagatcat gttccaacgc aatatcgaga
      721 gctgaactga aggaccaccg atgaatctca caccgcgcga aaaggacaag cttctgatcg
      781 ccatggcggc gatggtggcg cgccgccggc tggagcgcgg cgtcaagctc aaccatccgg
      841 aagccgtcgc actcatttcg gatttcattc tggaaggcgc ccgcgatggc cgcacggtgg
      901 ccgagctcat ggaaaaaggc gcgcatgtta tctcgcgtac ccaggtgatg gacggaatcg
      961 ccgaaatgat cccggagatt caagtggaag cgacttttcc ggacggcacc aagctcgtca
     1021 ccgttcacga gccgatacga tgaatttttc gttcttgaac aagacgtcgt catgttcggg
     1081 cgtgtcccgg ccatccacgt ctttcttccc aagtccgcaa tgagacgtgg atgcccgcga
     1141 aaagcgctgt cacgacaaga accaaaggac tctgtcgcaa tgatccccgg cgaatatttc
     1201 atccaggacg gcgagatcga actcaacaaa ggccgcaagg gcctcaccct caccgtctcg
     1261 aacactggcg accggccgat tcaggtcgga tcccattacc atttctttga gaccaacccg
     1321 gcgctgcgat tcgaccgcaa gaaggcgcgc ggcatgcggc ttgatattcc ggccggaacg
     1381 gccgtccgct tcgagcctgg tcagacacgc gacgtgaatc tcgtggcgct cgccggcacg
     1441 cgcaccgtct acggctttcg ccaggatgtc atgggaaagc tggaaaaagg aaagtcgcaa
     1501 aaatcatgac gagcggactt gcgcaagccc gcccgtcacg ttctcagcgc tgctaccgcg
     1561 gctgggacca ggatcaaatc agcacttggt gcgacgctgg tagcggcttc agcaccaagc
     1621 caactcgacc ttggcgatct ctctcattcg gtggaaccaa acgcgagcga tctggaaatc
     1681 atgatactgc agaagcggcg cgaagcaagc aaaagcgtgc atgcgcgcgc tggtgcaacg
     1741 cagtggggat aacgttgggg atttgttgaa gccggaggac ctgtatgacc cgtgcagcga
     1801 atgcggaagc tccaacaagg atggagatcg gtctggtttt gcaaggcgat ggcgcgctcg
     1861 gcgcctatga atggggcgcc atcaccgctt tgtttgatgt gatcgatgag gcaatccaca
     1921 acggccagga catcacgctc agggttgtca ccggcgtttc catcggcgcc atcaacgccg
     1981 cttgtgtggt cggggctgcc gaccgcgccg acgcgcgcaa ccggctgagt gcgatgtgga
     2041 gcgatttcat gatcgacctg ccgtatttgc cgccttcggc aaaccgcgac ctcgccctct
     2101 acaacgtgcc gcatttttac acgtatcgct ccgacctctt agccgcgccg acttggacct
     2161 acctctacga cacgcatcaa ttgctgcaga cattgtcgga ccacgtcgat tttaatgcgc
     2221 tcaacgccaa caagacggcg ttcgtgatta ccgcggtgga tgtagagagc ggtgtgctaa
     2281 cacggttcac caatcaaaag gtcggcgata ccgacccgac cgcgatcgag ccgcaccacg
     2341 tattggcgag cggcagcata ccgccgcaat ttccctggac cgacatcagc gccagcgaca
     2401 acacggtgca tcactattgg gacggcggca ttgtcgacaa cacgccgctt ggcgacgcca
     2461 tcgacgcttt cagctccgat cccggcgtta cgcggttgct ggtgatcatg aatctgttcc
     2521 cccaaaccgc gtctcttccc cgctcgctga cccaagtgaa cgagcggctg gatcaacttc
     2581 gcttcggcaa tcgcttgcgc caggacagca gcaccgcgcg gcagatgaat gcgctgatag
     2641 cgaccattca agatctttcg gcgctccttt ccgggccgct gcctgaagac ctgcaaaagc
     2701 gggtcgacaa agccaacgcc tacaagcttg tgaaactgat tgagatctcc ctgacagccg
     2761 acgcggtctc cgacgacccc aacagctttc gcgatttttc gcgcgaaggt attgtggcgc
     2821 gccgcgatgc gggccgccgg atcgctcttt ccgcgctacg gaccaccttc caaagcaaac
     2881 tcgccgcctg acaaggagct ttcgatgccg tctaagattt cacgttcggt ctatgccgat
     2941 atgttcggac cgacgacagg cgaccgcgta cggcttgccg acaccgacct catcatcgag
     3001 atcgagaagg atttttgcac ctacggcgag gaagtgaaat tcggcggcgg caaggtgatc
     3061 cgcgacggca tgggccagag ccagctcacc aacaaacaag gcgcggtcga taccgtcatc
     3121 accaacgcgc tgatcgtcga tcactggggc atcgtcaaag cggacgtggg catcaaggac
     3181 ggaaacattt acgccgtcgg caaggccggc aatccggaca ttcagcccgg cgtcaccatc
     3241 gtcatcggcc cgggcaccga agtgatcgcc ggcgaaggaa agatcctcac cgctggcggc
     3301 ttcgacacgc atattcattt catctgcccg cagcaggtcg acgaagcgct gatgtcgggc
     3361 gtcacctcga tgctcggcgg cgggaccggt ccggcgcacg gtacgttcgc cactacctgc
     3421 acgcccgggc cctggcatat cgcgcgcatg atccaggccg cggacgattt tccggtgaac
     3481 ctcgggttcg ccggcaaggg caacgcctca cggcccgcgg cgctcgagga aatgatcaag
     3541 gccggcgcct gcgcgctgaa gctgcatgag gattggggca cgacgccggc cgccatcgac
     3601 tgctgcctgt cggtcgcgga cgactacgac attcaagtga tgatccacac cgacacgctc
     3661 aacgaatccg gttttgtcga ggacaccatc gcggcattcg aggggcgcgc cgtgcactcc
     3721 ttccataccg agggatcagg tggcgggcac gcgccggaca ttatcaagat tgccagcctg
     3781 cccaacgtat tgccctcctc gaccaatccc acgctcccct acggcatcaa ctcgcaggcg
     3841 gagctctatg atatgatcat ggtctgccac cacctcagtc cggacatccc gtccgacgtg
     3901 gcattcaccg agagccgcat ccgcgctgag accatcgcag ccgagaacgt gcttcaagac
     3961 atgggcgtga tctcgatgtt ctcgagcgat tcgcaggcaa tggggcgtat tggggaatgc
     4021 tggctgcggt gcatccagac ggcagacgcg atgaaaaccg ggcgcggcaa gctgcccgag
     4081 gacgcaccgg gcaatgacaa tttccgggta cggcgctacg tggcgaagat cacgatcaat
     4141 ccggcaatca cgcacggcat cgccgacgtg ctgggctcgg tcgaggtcgg caagatcgcg
     4201 gacctcgtgt tgtgggagcc ggccttcttc ggcgccaaac cgaagatcgt gatcaagaac
     4261 ggcttcatca gctgggcggt gatgggcgac ccgaacgcgt cgctgccgac gccgcagccg
     4321 acctactatc gtccgatgtt cggcgcctac ggtgatgctt tggcggccag ctgcattacg
     4381 tttgtttccg gagcggccca cgctgccggg gtcaaggagc gcctcggact gcgcaggcag
     4441 gtgatgccgg ttcgtaacac gcgcaaaatc ggcaagcgcg acatggtttg caatgccgcg
     4501 acgccgaaaa tcgaagtgag cccggaaact ttcgccgtca cagtcgacgg caagcacgcg
     4561 accgtgccgc ccttgaaaac ggtctcgctg aaccagaaat atttcttcag ctgaagagcg
     4621 tgtcgtggtc gtcattgaaa ccgtactcgg caatatcagt gatccggaat gggccggtcg
     4681 gctggctgcg tccgaggtcg acgtgttgga gatcgaccag tgggaggcgc agaagagccg
     4741 cttccgcaag acgaccgcaa agggcgctga agttgcggtc tccgtcgatc gcggcacgta
     4801 catccgggat ggcgacgtgc tgctctggga cgcgcaggcg ctttcggccc ttgtcgcccg
     4861 catcgaggtg cgcgacgtaa tgatcgtgca tctggacgag ctgatgcacc tcgcgccgca
     4921 agtagcgatg cgcacctgcg tcgaactagg gcatgcgatg ggcaaccagc actggccagc
     4981 attggtcaag ggcgacctcg tctatgtgcc gctcacggtc gaccgcaagg tgatggcctc
     5041 cgtcatggag acgcaccact tcgaaggtgt ccgctacgag tttgtatcgg gccgagaggt
     5101 cgtgccctac ctcgcaccac atgaatcacg tcggctcttc ggcgcggcgg aaggtccggt
     5161 gcactcgcac acgcacgaga gctatgccac caccgacacc gagacgggac gggtgtacgg
     5221 acgcccgcca gtgcgcgtcc atcagcatcc ggagaccgat gctcatgcac atggatcgcc
     5281 gcccaaggcc ggtttcacgc acgaccaggg gggatgacag cgtcgaagcg ccaagtagca
     5341 ccgcattact tgcgaggatg ctgcagtttg gcgactcgat gttccccatc ggcgggtttt
     5401 cgttttcttg cggtttggaa tccgccatcc agaaaggcgt tgttgccgat gcaatcacgc
     5461 tccgtgcgtt cacgcgcacc gcagtggagc aggcagcgcg cggtgacggg atcgcgttga
     5521 tcgctgcaca ccgcgcagca acggtgggcg atgttgatgc actcatacga atcgatgcgc
     5581 aggtgtacgc gcgcaagctt tccgacgagg cccgactcat gtcggtgcgc atgggcaaga
     5641 agttcacgga aatgggtgtg gaggtaatcg gcgcgccgct gcttggcacg tggcgcaaat
     5701 gcattgcaat gtcggttact cccgggtgct atcccgtggc gctggcgatc agttttactg
     5761 tgcagaatct atcggcgcgc caagcgttcg tcgtgcacca gaatggtatc gcagcaacga
     5821 tcctaggcgc ggccctgcgg ctgatgaaga tcagccacgt cgagacgcag aagatcctct
     5881 acgagctcaa cggactcgcg gacgccgcct acgaaaacgt cgccgcggct cgcttgtcgg
     5941 acatggcggg ctatgcaccg ctgacggaaa tcctggcggc ggtccacaac aaggcgcacg
     6001 tgcgcctgtt catgagttga ccctttggtt ggagcatcga atgtcgaaga aaatcacgcg
     6061 cgtcggcata ggcggtccgg ttggatcggg caagaccgcg atcgtggaag ccattacgcc
     6121 gcgcttgctc gatcttggca tcaaggtgct cattatcacc aacgatgtgg tgacgaccga
     6181 agacgccaaa cacgtccagc gtactctcaa gggcgtcctg ctgcaggaac gcatcctcgg
     6241 agtggagacg ggggcgtgcc cgcatacggc ggtgcgcgaa gatccgagca tgaacctcgc
     6301 agcggtcgag gacatggagc gacgctttcc tgacagcgat gtggtgctga tcgaaagcgg
     6361 tggcgacaat ctgacgctca cgttctcgcc ggcgctggta gactacttca tctacgtgat
     6421 cgacgtcgcc gccggcgaga agattcctcg caagaacggg cctgggatca cgcattcgga
     6481 cattctggtc atcaacaaga ccgacttggc gccccacgtc ggggccagcc tggaggtgat
     6541 gaagcaggat tcacggctga tgcgcggcag caagccgttc ctgttcacca actgcaagac
     6601 cggggagggc attgcggaac tggtcgacct gatcaaaagg gagtttcttt tcgatatcaa
     6661 tccgcgagtg cagaggtcag catgataccg gcagcctcca ctccatgggc gcacgcggaa
     6721 gagctcggcg cttcggttcc ggaactggcc gcgttccagg acgagccgct tcagatgaag
     6781 agcggcacgg tcggcaagac tggtttcctg cgtctcggct tcgagcaccg ggccaacaaa
     6841 acaatcctgg caaacctgga gcgccgcgca ccgtatatgg ttcagcgcgc gttatattgc
     6901 gatccggaaa tgcctgatct cgcctgcgtg ttcttgatca ccaccaccgg atgcctgctg
     6961 cagggcgatc ggcttgcgct cgacatcacg cttggtccgc gcgcggaggc gcacgtcacc
     7021 acgcagtcgg caaccaagat acacgccatg gatgccaact acgcggcaca gacgcagatc
     7081 atcacgctcg ccgacgatgc ctacctggaa ttccttcccg atcccgtgat cccgcatcgg
     7141 caggcgcggt tcgtcagcga cacgcagatc tctatcgctc catcggccac gctgctcttt
     7201 tcggagatca ttcagcccgg ccgcaaacat catcgcccgg acgaatgctt tggcgccacc
     7261 gtgatatcga tatcgacgtc cgctgcccgc cccgatggct gccgcttgtt caccgaaaag
     7321 ctgctgatcg agccgcagcg atatgcaatg cggcagacgg gcgtgatgga ctcctttgac
     7381 gtattcgcca atgtgatcct gtgtacgccc aaagacaagg ctgatcgcgt ctatgagcgc
     7441 gtggaggccg atgtcgacct tgcagacggc gtggcgtttg gcgcctgccg gctgcccaac
     7501 gatgccgggc tgatctacaa gatcctgggt cgcgagaccg cacaggtaaa agccaaggta
     7561 cgcgagttct ggggggtggt gcgcagggag gtcactggcg ccggcctccc tccgccgttt
     7621 ctatggcgct gacaagaaca gcggcgtccg caggggtctg caactgcaga gctaaagctt
     7681 cgtggaggcg gcaatggaaa aattcttggc aaagtggcaa agcctctgcg catcatcacg
     7741 cgtcttgcgc ttcgtggaca tcaatctgcg cggcatcggg caggtgatgt ttcaggacaa
     7801 tccgctgacc ggggcgctct tcctgatcgc aatcgcctgg ggttcgtacg cagccggtgt
     7861 gccgcaagtt gcgattgccg gtgtggtcgc ggtcatcgtt gccacgctga ccgcgcaatg
     7921 gcttcgcgtc gacgacgagt cgctgaacgc cgggctctat ggcttcaacg ccatcctggt
     7981 cggcctcgcg ctggcgacat tcctggctcc cggaccgctg ctttgggtat atgtgatact
     8041 gggagcgacc gtatccgtcg tcgtgatgct tgccactacc aacgtcgtca agccatgggg
     8101 tggcgccctc acatttccgt tcgtgctcac aacgtggatt ctcttgttgg ccacgtatgg
     8161 attttccggc ctcgcgggaa cagcactgcc ggcgggcaag gttgtcaccg cgttccaacc
     8221 ctaccaagca agcccgctcc agctgattga ccttgtccag ggagtgttcc agagcatctc
     8281 gcaagtcttc ctgaaagcca gcggcatcgc cgcgttgctc ctgctcgccg ggctcgccgt
     8341 gaactcgctc gcagccgccg ccttcgctct cgggggtgcg atattggctg tgctgacggc
     8401 ccatcttttc ggcgccgaga gcgacctgat caccggcggt ctcctcggat tcagcccggt
     8461 cctgacggcg atcgcacttg gtaccgtctt ctatcagccg agctggcgcg tggcggtcta
     8521 cacggcgcta gccacggtgt tcacggtgat cgcccagtca gctttgaacg ttgcgttgac
     8581 accgttcgcc attccggctc ttacggcacc tttcgtgctg gtgacatggc tgttcctact
     8641 gccgcggcaa cgcctggagg aggtgcctgc cgtcgccccc gatatcgaaa agcctgccgc
     8701 cgcagcttga tccgcgttac gtttgggaat tccgtcttga ctgaaacggc cgtgacgacc
     8761 tgcacaagca cggaggcgac gatgccggca agttcaggcc ggcatgcaca cgtccaacgc
     8821 tcaagaatgg cgctcatcgc actttggcag gttctcggtt tgccgttggg tctgtgagtg
     8881 agacagcgat agtcaatcgt caacctggga tttgcgggag aaagcgaacc atgggcctca
     8941 ttgggatatt gcttggtcta gccctgctca tgtggctcgc gtaccggggg tggagcattc
     9001 tgctggtctc tccactggcg gcgctcgtcg cggctgtgtt ctcgggcgaa ccgctgctcg
     9061 cgcactggac gcagacgttc atgagcgggg cggcgcggtt cgtagcgcag tggttcccat
     9121 tgtttctgct cggcgggttg tttggaaagc tcatgggcga cagcggttcg gtcagcgcca
     9181 tcgcgcagta tttgaccaag aagttaggag ccagccgcac gatgctcgcg gtggtggccg
     9241 cctcggctat tgtgacttac ggcggcgtga gtgtgttcgt cgcatttttt gtcttggcgc
     9301 ccctggctca tgagatgttc cgggccgcga acctcccgcg ccggctgata ccggcagccg
     9361 tcggcctcgg tgcattcacg ttcaccatgt cggtgatgcc gggaactccc tcggtcaaca
     9421 acgccattcc gatgccgtac ttcggcacca ccacctttgc ggcccccggg ctgagtatca
     9481 tcgcgtcggc catcatcttc gtcttcggca tgtggtggct cggccgtgcg gaggcggccg
     9541 cgcgcaaggc tggcgaaggt tacgctggcg aggcgggaac cgctcctgtt gtcaacgaga
     9601 tggtccgcga gcaggcggcg ccggcgggcg acttcgaccc ggccgagtta ccgcatggca
     9661 agcaggccga gagcgggccg tccttcgtcc tggccatgct gcccatcctc gtcgtgatcg
     9721 tcaccaattt cctgatgtcg ctcgtcgtat tcccgcggct cgatttttcg ttcctgtccg
     9781 agcaccagtg gggcggtatt acgatcgggg ccgtgtccgg cgtctggtca gttttggtgg
     9841 ctctcgcggc ggcaaacttg accgtcattc tcatcaactt tcgacgcttg ccatctcccc
     9901 gcgaaagtct cgacgcgggt gcgaattctg ccgccttgcc gatgctgatg atcgccagtc
     9961 ttgtcggctt cggcgcggtc gttgccgcgt tgccggcatt tacgctggtt cgcgatgcgg
    10021 tattctcggt tcagggagga ccgctggtct cattggctct ctccatgaat gtgctggcgg
    10081 ggctgaccgg tactgcttcc ggcggcatgg caatcgtact caatgcattt ggcgccgact
    10141 ttatgcgtct cgccatcgag catcgcatcg atcccgactt gatgcatcgt ataacaacga
    10201 tgagcgccgg gaccctggac gccctccctc acaatggcac ggtgctgctg ttgctgcaga
    10261 tcagcggtct gactcaccgt gaaagctatc tcgacatggt gatgacggtg atcgtcagtt
    10321 gcatcatcgc acttgttgct gtcctgatac tgggttcggt ttttggatcg ttttgacgga
    10381 tgcaagggac gaggtcgcct gacgcagtta ttcacccctg aggggcagga aggctgtttc
    10441 aagcggaaac ctctgatcgc gttggaattg gtgacgaatg gagtgtcaag aataatcggg
    10501 gataatcatg tccataccct gcaaattcga acgcaacatt ctcagctaca atgagcatga
    10561 gatcgtcctg cgcagtcacc atcccgaaat ctatgatgcc gggcttgatg acctcagggc
    10621 gttgcgccaa cggctgcgcg atatgcgcaa caaggaaagg actctggcgc gcgaaaagcg
    10681 ccgcgaagca cgcggcaaag gggcgcggag aggcgaaagc tttccaggca cagccgaaca
    10741 tcccttgcag cgcaaacagg tgttcgccgc tgcgttgaaa cgcgtcaaca gggagatcga
    10801 tcgcatgcac aagctggagg cgcgggccgc gcatgtcgaa gcggcacgtc gcgctctcgc
    10861 catgcgccgt gcggctcaat ttcctgcgcg tcccccgagc ggcgacacgg caagcgaagg
    10921 gatgcgtgcg ctgccgagtc aacggcgccg tacacgagtt ccgcccgcaa ggatcggaag
    10981 cgtatcgcaa gcaacaaaaa ctgcgcaagc cgctcgcgac gcccgcaaca ggtctgtcca
    11041 gtgaacaagg agaatcacaa tgctcttctt cgcagaaggc ttcactttcc gcaattttgt
    11101 agccgacgtc ttcgcggtat ttttgttcgt cctctgattc tggctgctca tcatagtcat
    11161 aagcgatctg ttccggcgtc atgacatatc cggctgggtc aaggcgatct gggtgattgc
    11221 cctgatcgtg ttcccctatc tcggcatatt cgcgtacctg atcacccagt cgaggggcat
    11281 gaccgaacga agcagccagc aggcacaaca ggcgcgcgaa gagctgcggc gtgtggcggg
    11341 tttcagcgtt gccgacgaga ttgagaagct tgatcggctc aagaaatcag gaacgatcac
    11401 cgatcaggaa ttcacgcgtc ttcgcgccaa gctggttgca tagtcgcccg cccaccatcg
    11461 cggtcgttcc gacgtcggag cgcgccatgg atcgcgcaga gagcgcacgg gaaaaccgag
    11521 tcacccgaat acgtaggaga gaatctgaat tagggcggtc agcgctgagc atatcaagag
    11581 cacgagccag aaccatttcc agctccaggc tgacagcccg gcgcctatgg cagcgaccaa
    11641 cgacggagca aatactccta tgtggtacag gattccgcgg cggactgggc ttgtgcccag
    11701 gtaaaagctg atggcagcaa ccagtgcgat caggactaca gcgcgagccg ttggactctg
    11761 cacaatggaa ttaatgacac tttgcattga gcaagcttgg caaagttact gtacggcttc
    11821 aagaaggcga acgatggctg gttcagagac gacacatcct ccagcaagaa gttctgcacg
    11881 tgttgaaatt ggtaaaacaa ttcgggccgg gatggcggct ttcgctcggc gatcgctccg
    11941 tgctcagcgc tgttgtgtgc tcccaagcgg atcgggctgg acagcggagt gagccgccga
    12001 gcgcgacttg gttatgccga taggcgcctc gttggatggc agcactcaaa gttggtcttc
    12061 gccgtggctg gtgttggaac agcggtcgcg gactaccgtt tggagaggtg ctgcttcagc
    12121 cgcgtgatcc cttccggggc ccagtcgcga acgtctgccc gctcccccgg ccatgggggc
    12181 gcttccaggc ctatgcggcg ttgacgctaa aggtggcggc aatgcaagga attgttcaaa
    12241 cagtcggttc gaacaatatt caagaaaagc caaccgactg cgcaaccatg gatcccgatc
    12301 atcttgtctg ctggaacttt atgtgcaaag ccgccggagg gcgaggtcaa gaaggacgga
    12361 aagcctgcgg gcaagcaacc cgtgctcgac ctcatggggc gtgtctccgt gctctgcaac
    12421 gacgctgagc tgttcgaaga agatggcgcc tggaaggtgg aaggagaccc cctaaaaggg
    12481 ggtagccttt ctgacggaag gcgtttttga aaaaaaattt tcaggtgccg gagaggattc
    12541 cgctgtcggc gctagtgcca gcacatatgg cagcgccgct accggggcga ggcagcgcga
    12601 gcgccggtgg gatggccgac gtttgcccga ccgcctcgat ggctcgaagt gcgcggacta
    12661 aacgactgcc atgacaaaga ggcaaccagc cgcgacacat ccgaaggccg ctccccgcca
    12721 gttgttgtcc atcg
//
LOCUS       DATLJT010000054         6432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3218754,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000054 DATLJT010000000
VERSION     DATLJT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218754"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /locus_tag="VM910_01085"
     CDS             complement(<1..2162)
                     /locus_tag="VM910_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI61179.1"
                     /translation="MGARLRAHDWVNTPLGEPQHWPRSLKTAVRIMLTSRQPIWIGWG
                     GELTYLYNDAYKSIIGGKHPWALGRSTAEVWREIWHEIGPMLATAMKGDEGTYVEEQL
                     LIMERSGYREETYYTFSYSPIPDDEGKAGGIICANTDDTRRVIGERQLALLRELAAAA
                     ADVRTLEGACRRAAQALATDPKDLPFAMIFLVERDGRTISLVGSTGFSADHPAAPASV
                     AIDTPSLWLLSDVIRGQEPRVVSHLVPRFGAEFPKGFWDQSPNQATLIPIPARGETAR
                     KGVLVAGLNPFRAFDDTYRRFLELAAGEIGASIANAQAYEEERRRAEALAEIDRAKTL
                     FFSNISHEFRTPLTLMLGPIEDALNDASATALNGAQRERIEVAHRNSLRLLRLVNSLL
                     DFSRIEAGRMRASFAPTDLAAFTAELASNFRSAMDRAGLKLKIECSPLPHPVYVDREM
                     WEKIVLNLVSNAFKFTFEGEISIEVRSVENGAAAELAVRDTGSGIPSHELPRLFERFH
                     RIEGAKGRTFEGSGIGLALVHELVHQHGGTISVKSELGRGSVFAVRLPFGDRHLPREH
                     VAVQAPAPPAVGSRADAYVHEALRWLPEAIAEELQSALATELSDETLVARGEGERIIL
                     ADDNADLREYVRRLLAAQGYIVEAFSDGEAALEAARRKRPDLILSDVMMPRLDGFELL
                     AQLRQDQRLRDLPIILLSARAGEEARVEGLDAGADDYV"
     gene            complement(2341..2562)
                     /locus_tag="VM910_01090"
     CDS             complement(2341..2562)
                     /locus_tag="VM910_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61180.1"
                     /translation="MIADGFDFRTLIRMDKALERVCRGRPGGEGHELRKQVAESILVS
                     ARGGRTGFGQLTEAGERALTGVWRVTRHV"
     gene            2748..3104
                     /locus_tag="VM910_01095"
     CDS             2748..3104
                     /locus_tag="VM910_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61181.1"
                     /translation="MIEGISAITLATHDMPRAVRFYRALGFDVLYGGEASSFTSFRAG
                     AGYLNLIAQPDEKHWSWWGRVIFYVDDVDALYGRALAAGFQPSTVPRDAEWGERYFHL
                     TDPDGHELSFARPLPA"
     gene            complement(3131..4132)
                     /locus_tag="VM910_01100"
     CDS             complement(3131..4132)
                     /locus_tag="VM910_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HVI61182.1"
                     /translation="MTNKDQMQAALLDGPKQPFRVASVVRPTPKSGEVLVRIKASGVN
                     PLDLKIRAGQAAHARHPLPAILGIDLAGTVEAIGPGVAAFREGDEVYGMTGGVGGVQG
                     SLAEYAAVDAALLARKPSNLAMREAAALPLIFITAWEGLVDRAKVQAGQKVLVHGGAG
                     GVGHVAVQLARARGAQVFATGSAKSTAFIAQIGATPIDYNVMTVDDYVARYTGGRGFD
                     VIYDTVGGTTLDASFNAVRRFGHVVSALGWGTHALAPLSFRAGTYSGVFTLLPLLTNE
                     GRAHHGEILREATGLVEAGKLRPRLDPRRFGLGSAGAAHAAIEDRSAEGKIVIDIAG"
     gene            complement(4186..4965)
                     /locus_tag="VM910_01105"
     CDS             complement(4186..4965)
                     /locus_tag="VM910_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI61183.1"
                     /translation="MQMNRRAFSAALVAGTAASLIATRGTVAMPAPPKARNVVLVHGL
                     FADGSCWSEVIARLQAAGLNVTAVQNPLTTLPEAVASAERVLARQDGPTVLVGHSFSG
                     MIVTEAGVHPNVSALVYVAARAPDAGEDYTALAKTYPTPPATAGIVFDGDEGRLGEEA
                     FLRDFAGDLPPAKARVLYAVQEPFHKALLTGKTTQAAWRSKPSYYAVSTEDRTINPDL
                     ERFMARRMGAKTIEVKASHLSLISHPDEIADLIVEAAGKER"
     gene            complement(5041..5424)
                     /locus_tag="VM910_01110"
     CDS             complement(5041..5424)
                     /locus_tag="VM910_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HVI61184.1"
                     /translation="MIRGYSDHAANERTFLAWVRTGIAIVVFGFVIEKFNLFLVMIAN
                     ADAVNGRRAMLERLSGPVGRYEGMALVLVGLTLIVAAAVRFIHVRRLLADQETHEVGG
                     YGAELVLSAVLALAVVAYSIYLLVR"
     gene            complement(5472..5951)
                     /locus_tag="VM910_01115"
     CDS             complement(5472..5951)
                     /locus_tag="VM910_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HVI61185.1"
                     /translation="MARPRPKWAAGLAAIALIWLWGVHAMAADGLITIKSAFGPEETM
                     QRLEAEVRAKGMTVFAHVDHAAGAAAVGLPLRPTDLLIFGNARGGTPLMQSAQAIGID
                     LPLKALVWQDASGATWLSYNDPAWLAHRQGVGEEAKPIVNALTAALNALATKATTPP"
     gene            complement(5979..6122)
                     /locus_tag="VM910_01120"
                     /pseudo
     CDS             complement(5979..6122)
                     /locus_tag="VM910_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
BASE COUNT         1208 a         2062 c         1948 g         1214 t
ORIGIN      
        1 ttgacgtaat catcggcgcc cgcatcaagc ccctcgaccc gcgcctcctc tccggcgcga
       61 gccgacagga gaatgatcgg caggtcgcgc agtcgctggt cctgccgcaa ttgcgcgagc
      121 agttcgaacc catcgaggcg aggcatcatg acgtcggaaa gaatgaggtc cggcctcttg
      181 cgccgcgcgg cttcgagcgc cgcttcgccg tcggaaaacg cctccacgat ataaccctgg
      241 gcggcgagca ggcgccggac gtattcacgc aggtcagcgt tgtcgtccgc cagaatgatg
      301 cgctcgccct ccccgcgcgc gacaagcgtc tcgtcggaca attcggtcgc caaagcggat
      361 tgcagttctt ccgcaatagc ctcgggcagc caacgcaagg cctcatgaac ataagcgtcc
      421 gcccgactgc cgactgccgg cggtgccggc gcttgaacag cgacatgttc acgcggcaaa
      481 tgccggtcgc cgaacggaag cctgacggcg aagacactgc cgcggcccag ttcgcttttg
      541 acactgatcg tgccgccgtg ctggtggacc agttcgtgga ccaaagcgag cccgattccg
      601 ctaccctcga acgtcctgcc cttggcgcct tcgatccggt ggaaccgttc aaacagccgc
      661 ggcaattcat ggctcgggat gccgctgccc gtgtcgcgaa cggcgagttc cgcggctgct
      721 ccattctcca ccgagcgaac ttcgatggaa atctctccct cgaacgtgaa cttgaacgcg
      781 ttggacacga gattgagcac aatcttttcc cacatctcgc ggtcgacata gacgggatga
      841 ggtagcgggg aacactcgat cttcaatttc aacccggcgc gatccatggc ggatcggaag
      901 ttggacgcaa gctccgcagt gaaggccgcg aggtcggtgg gagcgaaact tgcccgcatc
      961 cgtccggctt cgatgcgaga aaagtcgagc aagctgttca ccagccgcaa cagtcgcaac
     1021 gagttgcgat gcgcaacttc gatgcgctcg cgttgggctc cattcaaggc tgttgcggag
     1081 gcgtcgttga gggcgtcttc gatcggcccg agcatcagcg tcaacggcgt ccggaattca
     1141 tgactaatgt tcgagaagaa cagagtcttg gcccgatcaa tttcagccag agcctcggcg
     1201 cggcgccgct cttcctcata ggcctgggcg tttgcgatgc ttgcgccgat ttcgccggcc
     1261 gccagttcga gaaagcgccg atacgtgtcg tcaaatgccc gaaacggatt gagacccgca
     1321 acgagcacgc ccttgcgtgc ggtttcaccc cgcgccggaa tgggaatgag cgtggcctgg
     1381 ttcgggctct gatcccaaaa gccttttgga aattcggcgc cgaaacgcgg tacgagatgg
     1441 gaaaccactc gaggttcttg cccgcgaatg acatcggata gcagccacag cgacggcgta
     1501 tcgattgcaa ccgaggccgg agccgcgggg tgatccgcgc tgaaaccggt agaccccacg
     1561 agggatatgg ttcggccatc ccgttcgacc aaaaagatca ttgcgaaggg cagatccttc
     1621 ggatcggtcg ccagcgcctg ggcggcacga cggcacgccc cctccagggt ccgcacgtcg
     1681 gccgccgcgg cggcaagctc gcgcaaaagg gccaactgcc gctcgcctat gacgcgacgg
     1741 gtatcatcgg tattggcaca gatgattcct ccggccttac cctcgtcatc gggaatcggg
     1801 ctgtaggaga acgtatagta cgtctcttct cgatatccgc tgcgctccat gatcaggagc
     1861 tgttcttcga cgtaggtgcc ttcgtcgccc ttcatggcgg tggccagcat cggaccgatt
     1921 tcgtgccaga tttcgcgcca gacttcggcg gtggaccggc caagagccca gggatgcttt
     1981 ccgccgatga tcgacttgta ggcgtcgttg tagagataag tgagttcgcc tccccagccg
     2041 atccagatcg gctgacgcga cgtcagcatg atacgaacag cggttttcag gctccgcggc
     2101 cagtgttgcg gttcgccgag cggagtattg acccagtcat gagcgcggag gcgtgcgccc
     2161 atctcgcctc caccggaaag aaaatcagag gcggagggga tcgccagatt tctgaactcg
     2221 tggagattgc tcaaacgggc tccaggctgt ttcgcgatcg gatctcctcc cgcgcaacgc
     2281 ccgatagaac tccggacggt cgcaggttgc tggcgcgaca aagagggaga ggggctgtgg
     2341 tcagacgtgt ctggtcacgc gccatactcc ggtaagggcg cgttcgcccg cttcggtgag
     2401 ctgcccgaag ccggttcttc cgccccttgc ggaaacgagg atgctttcgg caacctgctt
     2461 gcgaagctca tggccctccc cgccgggcct gccgcgacag acccgctcca gcgccttgtc
     2521 catgcgaata agagtgcgaa agtcgaagcc atctgcgatc atttttgccg cccggcgggc
     2581 cctcccgatc attgctgccg gcatcacaat cgtgaaaggg caactttggt tccaacccaa
     2641 aagcgtgtcc cgcagcttcc tgtcgatcat tacactcggc tgcccggtct ggtcatggcg
     2701 acgccctgcc ctgttgcccc cggtcttcaa cccatggagg tgtcgccatg atcgaaggca
     2761 tcagcgccat cacgcttgca acccacgaca tgccgcgcgc cgtgcgattt taccgcgcgc
     2821 tcgggtttga cgttttgtac ggtggcgaag cgtcgtcgtt caccagcttc cgcgccggcg
     2881 cgggctatct caacctgatc gcccagcctg acgagaagca ctggtcctgg tggggacgcg
     2941 tcatcttcta tgtcgacgac gtcgatgcct tgtacgggcg tgctctggcg gcaggatttc
     3001 agccctcgac cgtgccccgc gacgccgagt ggggcgagcg ctactttcac ttgaccgacc
     3061 ccgatggcca cgagctcagt tttgcacgtc cattgccggc gtagagcgca caagcgcgcc
     3121 cggcggcaaa tcatcctgcg atatcgataa caatcttgcc ctcggccgat cgatcttcga
     3181 tcgcagcatg cgctgcgccg gcgctgccca agccgaagcg acgcggatcg aggcggggcc
     3241 gaagctttcc ggcttcgacg agtccggttg cttctctcag aatctcgccg tgatgcgcgc
     3301 gaccttcatt cgtcaggagt ggcagcaggg taaacacccc ggaatacgtc ccggcacgaa
     3361 acgacagagg cgccagcgca tgcgtgcccc aacccagcgc gctcacgaca tggccgaagc
     3421 ggcgcacggc gttgaacgat gcgtcaagcg tcgttccgcc gacggtgtca tagatcacgt
     3481 cgaagccgcg accacctgtg tatcttgcga catagtcatc gaccgtcatc acgttgtagt
     3541 cgatcggggt tgcgccgatc tgcgcgatga aggctgtgct cttcgccgaa ccggtcgcga
     3601 acacctgtgc accccgtgcg cgcgcgagtt gaaccgcgac atggcccacg ccaccggcgc
     3661 cgccgtgaac caggactttc tggccggcct gcaccttggc gcgatcgacc aacccttccc
     3721 atgccgtgat gaagatcaat gggagagcgg ccgcctcgcg catcgccagg ttcgaaggct
     3781 ttcgcgcaag cagcgctgca tcgacggccg cgtattcggc gagtgagccc tgtactccgc
     3841 cgacgccgcc cgtcatgccg tagacctcat cgccttcgcg gaaagccgca acaccgggac
     3901 caatggcttc gacagtcccg gcaaggtcga tacccaatat cgcgggcagc gggtggcgcg
     3961 cgtgagcggc ctgaccggca cgtatcttca gatcgagcgg attgacgccg ctcgccttga
     4021 tgcgcaccag cacctcgccg gatttgggcg tggggcgcac gaccgatgcg accctgaagg
     4081 gctgcttcgg gccatcgaga agtgccgctt gcatctggtc tttgttcgtc atgagcaggt
     4141 ttctccggct ggaacggctt aactcgtgat tgctcaggca actcgctatc gctcttttcc
     4201 cgcagcttcc acaatcaggt cggcgatttc atcgggatgc gagatcagcg agagatggct
     4261 tgcttttact tcgatcgtct tggctcccat ccgcctggcc atgaagcgct ccaggtcggg
     4321 attgattgtg cggtcttctg tggaaacggc ataataactt ggcttcgatc gccatgcagc
     4381 ctgcgtagtt ttgcccgtga gcagtgcctt gtggaacggc tcctgaaccg catagagcac
     4441 cctcgccttt gccggcggca ggtcgcctgc aaaatcacgc aggaatgcct cctcgccgag
     4501 acgtccttcg tcgccgtcga aaacaatccc ggcggtggca ggcggcgtcg gatacgtctt
     4561 cgccagcgcg gtgtagtcct cgccggcatc cggcgctcgc gccgccacgt agaccagcgc
     4621 ggagacgtta ggatgcactc cggcctccgt gacgatcatc ccggaaaagg aatgaccgac
     4681 gagaaccgtc gggccatcct gccgcgccag cacccgctcg gccgaagcga ccgcttcggg
     4741 cagcgtcgtt agcgggtttt gcacggccgt cacgttgagc cccgccgcct gcaatcgcgc
     4801 gatcacttcg gaccagcatg atccgtcggc aaacagcccg tgcacgagca cgacgttgcg
     4861 cgcctttggc ggagcgggca ttgcgaccgt tccacgcgtg gcgatcagcg aagcagccgt
     4921 accggcaacc agagcggccg agaatgcacg tctgttcatc tgcatggaat cctccggcca
     4981 ttcgtttcga gtcattcggt gtcaggaaaa gagcgcctac cgcattctcc aacacctgcg
     5041 tcaacggacc agcaggtaga tactataggc gaccaccgcc agtgccagca ccgccgagag
     5101 cacgagttcc gcgccgtagc cgcccacctc atgcgtttcc tgatctgcaa gcaatcggcg
     5161 aacgtggatg aagcggaccg cggcggcgac aatcagcgtc aggccgacaa gaacgagcgc
     5221 catcccctca taacggccaa ccgggccgga cagcctttcg agcatcgccc ggcgtccgtt
     5281 cactgcgtcg gcgtttgcaa tcatgaccag gaacaggttg aacttctcga tcacgaatcc
     5341 gaacacgaca atcgcaatgc cggtccgcac ccaggcgagg aaagtacgtt cgttggcggc
     5401 atgatcgctg tagccccgga tcatgaatca cctcactgcg gtttgtggtt atccgaacgg
     5461 cgcggccgct gtcatggagg cgtggtcgct ttggtcgcga gcgcattcaa cgcggccgtc
     5521 aaggcattca ctatcggctt tgcttcttcg cccacgccct gacgatgcgc gagccaggcg
     5581 ggatcattgt aggagagcca ggtcgcgccc gatgcgtcct gccagaccag cgccttcagg
     5641 ggcagatcga tgccgatggc ttgcgccgat tgcatcaacg gcgtgccgcc cctggcgttg
     5701 ccgaagatca acagatcggt ggggcggagc ggcaggccga cggctgcggc tcccgccgca
     5761 tggtcgacat gggcaaacac cgtcatgccc ttggcgcgga cttcggcctc gagccgttgc
     5821 atggtctctt ccggcccaaa ggcgcttttg attgtgatca gtccgtctgc ggccattgcg
     5881 tgcactcccc atagccatat caaggcgatc gctgcgagac ctgccgccca tttcggtcgc
     5941 gggcgcgcca tctcatttcc tgccgaatga ccgcgccggt cgaattccgg gtcgggtacg
     6001 aatccgccgc gattgggcat cttgatcttc ggcaggcttt cacggtcgag ctttccctcc
     6061 gcaggaacaa tgccgttcaa gctcaggatg taggcggcga ccgcataggt gtcgtcatcg
     6121 ctcggcaagc gaggggattc aacggcgtcg ttcggaaatt cgggctgatc ggtcatcgct
     6181 atctccgaag accatcgatg aaaagatcat atctcccgcg agggccttga tgcaggttcg
     6241 tcctggaccg gtcgcggcat tgcgcggacg gcaacagaac gccgcaggcc gctgttgatc
     6301 taaatcaaca taagtcgtcc ttgggtcacg accgggcgaa agccaattgc gttctggagg
     6361 tcaagagcgc aacgtcgagt tcgaaacctt tggcaacgtc aaggacccgc gcgtcgcagc
     6421 aagatgagca aa
//
LOCUS       DATLJT010000055         8506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1840075,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000055 DATLJT010000000
VERSION     DATLJT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840075"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2648)
                     /locus_tag="VM910_01125"
     CDS             complement(<1..2648)
                     /locus_tag="VM910_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI61186.1"
                     /translation="MTAFAQPTPTRKRSSLEQQAATSEVLSVISRSPGDLSPVFDAML
                     ANATHICEAKFGVMYRSEGDAFRAVALHGAPAAYAEERRRNPIIRPNPNSTLGRTVAT
                     KQAVQITDVLEEPGYLDPPSGYYVPQLGKLAGARTVLGVPMVKDNELMGAILIFRQEV
                     RLFTDKQIDLVSNFAKQAVIAIENTRLLNELRQRTDDLSEALEQQTATSEVLGVISSS
                     PGELEPVFQSMLANAIRLCEAKFGNLYLCEGDAFRTVALHGATPEYTEARRRAPLIRP
                     AANTGLRRVLETNQVVQIADVQAVAGYLDNPVQAPFVQLAGGRSLLSVPMLKDDELVG
                     AIEIYRQEVCPFTDKQIALVQNFASQAVIAIENTRLLNELRESLQQQTATSDVLKVIS
                     RSTFDLQAVLNTLVESAARLCEADMASIARQKGANYHLVATHGFSASFKEYMETLPME
                     PGRGSGTGRALLERKPVHIIDALADPEYTMAEAQKKGGWRTMLAVPLLREGTPIGVLH
                     LLRTVVRPFTDKQIELIETFADQAVIAIENVRLFEQVQARTRELSEALEQQTAASEVL
                     RVISSSPGELAPVFQTMLANAVRICEGKFGVMFGFADGAFRALSSLGAPPPPLTQQPH
                     VVSEHPHNPLTRLAESKAVVHIPDLTVDQAYIERNPRIVALVESGGARNLLLVPMLKD
                     DELVGAIAIYRQEVRPFTDKQIELVTNFAAQAVIAIENTRLLNELQESLQQQTATADV
                     LKVISRSTFDLQAVLDTLIESAVRLCEADMGSINRQRGDVYRQVANFGHSPALDKFME
                     THPLEISRGTVVGRTVLEGRTVHIADVQADPEYAFLEAARIGGLHTMLGIPLLREGTP
                     IGVIVLQRKSVQPFTEKQIELATTFA"
     gene            complement(2857..3234)
                     /locus_tag="VM910_01130"
     CDS             complement(2857..3234)
                     /locus_tag="VM910_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61187.1"
                     /translation="MKRRDTGPLTVVWILLTASAALAAEPMAPSDIQATFFNGQPFTA
                     STPGGTKFRMTFTPDGKMTREPLAESGHKNNGTWKLNTKGFCTTWQHAKSNCFTVIPK
                     GENSWSVQTIATTIATTVAVWSK"
     gene            complement(3231..4208)
                     /locus_tag="VM910_01135"
     CDS             complement(3231..4208)
                     /locus_tag="VM910_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61188.1"
                     /translation="MRRREFITLLGGAAALPLAARAQQARLPIIGYLNAQAPAAVETA
                     RNKTAFQQGLREAGYIEGQNVVIEYRWAEGRYERLPALAAELTGRSVAVIAATGSDFS
                     VRAAKAATTTIPIIFSTASDPVQSGFVASLNRPSGNVTGVTSLGVEVAPKRLQLLREV
                     VPAANIFALLVNPINTNAETISRQTQAAAQTLGVQLHVVHASTEREIETALASLRASA
                     LVIGSDGFFTTRIEQLGAQLLRHAVPSIYAGREYAVAGGLMSYGEDFRDMYRHIGVYA
                     GRILRGEKPADLPVLQATKVELIINMKTAKALGLTVPITLLGRADEVIE"
     gene            complement(4208..5182)
                     /locus_tag="VM910_01140"
     CDS             complement(4208..5182)
                     /locus_tag="VM910_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61189.1"
                     /translation="MKRREFIITLLGCAAAWPLAARAQQSATMPVIGLLDQRSPDVLA
                     DRLRAFRQGLKEAGLIEGQNVAIEYRWAETDRLPEVARDLVHRQVDVIVATGALPTVL
                     AAKTATTTIPIVFVIAADPVKLGLVASLARPGGNLTGINFLTGELTAKRLALLRELVP
                     AASRVAVLINPTNTENNEREIETIARTMGLQVQLLNASTIHEINAAFATFTRERPDAL
                     FVGLDPFFNSRRTQLVHLATRHAIPASYAGREFVEAGGLMSYGANISDAWRQAGPYVG
                     RIVKGAKPADLPVVQSSKFELVFNVQTATMLGITVPQTLLATADEVIE"
     gene            complement(5179..6165)
                     /locus_tag="VM910_01145"
     CDS             complement(5179..6165)
                     /locus_tag="VM910_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61190.1"
                     /translation="MKRRQFITLLVGGAAAWPLAARAQQGERMRTIGVLNALATDAPE
                     GVARLAAFVQGLQQLGWTEGRNVRIETRWAADSDHLRRYAAELVALAPDVILASGGTV
                     VGPLLQATRTVPIVFTLAIDPVGGGWVDSLAQPGRNATGFTSYEYSLTAKWLELLKEI
                     APGVTRVAVLRDANSPQGVGQFAVLQSVASTLSVELRPLDVSDPGEIERGITAFTQTP
                     NGGLIVTGSSWAFVHRDLILALAARHRLPAVYFQHHFVAGGGLISYAPDDIEPHRRAA
                     GYVDRILRGEKPADLPVQASTKYRLAINLKAAKALGLEVPATLLARADEVIE"
     gene            complement(6165..7172)
                     /locus_tag="VM910_01150"
     CDS             complement(6165..7172)
                     /locus_tag="VM910_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61191.1"
                     /translation="MSDMRRRQFITLLGGAAAAWPLAARAQTAASMRSIGVLTALAEN
                     DVGWQRNFAGFVAGLSEKGWLEGRSFRFLFRYVPGGNLDHLRMAAGELVSLAPDVILA
                     VNPYSARFVADRTREIPVVFLLSIDPVAEGLVASVSRPGGNVTGFTQSDYSLGGKWPQ
                     LLKEMAPSVTALLVIVGGDASWSSWSAAYLRSIEQAAQTLNISVTAIEIHNQSEIEQK
                     IIALGKPNLGLIFPPNSFTSDHRPEIIELVTRLRLPSIYFARFFVLDGGLMSYGIDQP
                     HLFRQAGSYVDRILRGTKPADLPAQFPTKFELVINIKTAKKLGLNVPDALLARADEVI
                     E"
     gene            7451..>8506
                     /locus_tag="VM910_01155"
     CDS             7451..>8506
                     /locus_tag="VM910_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI61192.1"
                     /translation="MGHLDPTQNSQAASEKKLKASACELEQKLEARTRELAEAREQQT
                     ATSEILRVISSSPGDLKPVFETMLENATRLSEAKFGFLFLREGDAFRAVAMHGPPPIV
                     EWMQQAPVVELRDHPHVPFARIARTKEALHVSDLTTEQSYIERDPRIVALVEAAGARS
                     FIAVPMLKEEQLIGAMAIYRQEVRPFTDKQIELVQNFANQAVIAIENTRLLNELRESL
                     QQQTATADVLKVISRSTFDLQAVLETLVENAVRLCDADRGIINRQDGDLYRPAASFGN
                     PPEWIEIMERNPIRKDRTSAVGRAILERRVVHIHDILADAEYRWAEEHRGEKEMHRTI
                     VAVPMLREGAVIGVIVIR"
BASE COUNT         1653 a         2580 c         2595 g         1678 t
ORIGIN      
        1 tcggcgaacg tggtggccag ttcgatctgc ttctcggtga acggctggac gctcttccgt
       61 tgcaggacaa tcacgccgat cggcgttcct tcgcgcagca acggaatgcc taacatggta
      121 tgaaggccgc caattcgtgc agcttcgaga aaagcgtatt ccggatcggc ctgtacatct
      181 gcgatatgga ccgtccggcc ttcaagaact gtccggccga caacggtccc tcgactgatt
      241 tccaacgggt gggtttccat gaacttgtca agcgcgggcg agtgaccgaa attggcaact
      301 tgccggtata cgtcgcctcg ctgtcgattg attgatccca tatccgcctc gcacaagcgg
      361 acagctgatt caatcagcgt atccagcact gcctgaagat cgaacgtcga gcggctgatc
      421 accttgagca cgtcggcggt ggcggtctgc tgctgcagcg attcttgcaa ctcattgagg
      481 agtcgcgtgt tctcgatggc gataaccgct tgcgcggcaa aattggtcac gagttcgatc
      541 tgcttatccg tgaacgggcg aacctcctgt cgatagatgg cgatggcgcc gactagttcg
      601 tcatccttga gcatcggcac aaggagaaga tttcgcgcac cacccgactc gaccagggcg
      661 acgatacggg gattccgctc gatgtaagcc tggtccacgg tgaggtcggg aatgtgcacc
      721 accgctttgc tctcggcgag gcgtgtgagc ggattatgcg gatgctcgct cacgacgtgc
      781 ggctgttggg tgagcggcgg cggcggcgcc cccagcgacg acagcgcacg aaatgcgcca
      841 tcggcaaacc cgaacatgac gccgaatttg ccttcgcaga tgcgcaccgc gttggcgagc
      901 atggtctgaa acacgggcgc tagctcgccg ggcgagctcg agatgacgcg cagcacctcc
      961 gaggccgccg tctgctgctc cagcgcctcg cttagttcgc gcgtgcgcgc ttgtacttgc
     1021 tcgaaaagcc gcacgttctc gatcgcgatc accgcctggt cagcgaaggt ctcgatcagt
     1081 tcgatttgct tgtcggtaaa aggccggacg acagtgcgga gcaagtggag caccccaata
     1141 ggagttccct cgcgcaggag cggtacagcg agcattgtgc gccagccgcc tttcttctgc
     1201 gcttcagcca tggtgtattc cggatcggct aaagcgtcga tgatatgaac cggtttgcgc
     1261 tcaaggagag cgcgcccagt tcccgacccg cgacccggct ccatcggcaa ggtttccatg
     1321 tattctttaa agctggccga gaacccgtgc gtcgccacaa gatggtagtt cgcccctttc
     1381 tggcgagcga tcgacgccat gtccgcctcg caaaggcgag cggccgattc gacgagcgta
     1441 ttcaataccg cctgaagatc gaaggtcgag cggctgatta ccttgagcac gtcggaggtc
     1501 gcggtctgct gttgcagcga ttcccgcagc tcgttgagca agcgggtgtt ctcgatggcg
     1561 atgacggctt ggctggcaaa attctggacc agcgcgatct gcttgtcggt gaaggggcaa
     1621 acctcctggc gatagatttc gatggccccg accaactcgt catccttcag cattggaacg
     1681 cttagcaggc tgcgcccgcc ggcaagttga acaaacggcg cctgcacagg attgtccaaa
     1741 tagcctgcca ccgcctgtac gtcggctatt tgaactactt ggttggtttc cagcacgcgc
     1801 cgaaggcctg tattggcagc gggccgaatt agtggcgcgc gccgtctggc ctcggtgtat
     1861 tcgggcgttg cgccatgcaa ggccaccgtg cgaaacgcgt ctccctcgca cagataaaga
     1921 ttgccgaact tggcctcgca gagccggata gcgttcgcca gcatggactg gaaaacgggc
     1981 tccagctcgc caggcgaact cgagataacg ccaagcacct cggacgtcgc cgtctgctgc
     2041 tccaatgcct cgctcagatc gtccgtgcgc tggcgcagct cgttgagcag ccgcgtgttc
     2101 tcgatcgcga tgacggcctg cttggcgaaa ttgctgacca gatcgatttg cttatcggtg
     2161 aagagccgga cctcttggcg gaagataaga atggcgccca tcagctcgtt gtccttgacc
     2221 atcggaaccc cgagcacggt ccgggcaccg gcaagcttgc cgagctgcgg aacgtagtag
     2281 cccgacggcg gatcgagata tccgggttcc tcaaggacat cggtgatctg gaccgcctgc
     2341 ttagtcgcca ccgtgcgtcc gagcgtgctg ttcggattcg gacggattat tgggttacgg
     2401 cgccgctctt cagcataagc ggccggtgcc ccatgcagtg caacggcgcg aaacgcatcc
     2461 ccttcggatc gatacatgac accgaatttt gcttcgcaga tgtgggtcgc gttcgccagc
     2521 atggcgtcga acacgggaga gaggtcgccc ggcgacctgg agatgacgct cagcacctcg
     2581 gaggtcgccg cctgctgctc cagcgagctt ctttttctgg tcggcgttgg ctgcgcgaag
     2641 gcggtcaacg tgagtcctcg ccttcgttgt cttcgaacga agactggtaa tccgtgttcg
     2701 gctcttcgcc cctttgcgag ccaaggtcac ctcgtcggca cagtcactgg tcccccttcc
     2761 ggtcggccca gccaaggatc gggcctacca atcctaacgc acgatgaact cgcgcctctt
     2821 cattcgatca cctcgtcggc gcgggcgatt agatcgctat ttagaccaca ctgccaccgt
     2881 ggtagcgatt gtggttgcaa ttgtttgaac cgaccaactg ttctcgccct tcggaatgac
     2941 ggtgaagcaa ttagacttcg cgtgttgcca ggtcgtgcaa aagcccttcg tattaagttt
     3001 ccacgtacca ttgttcttgt ggccggattc tgcgagaggc tcacgggtca tcttgccgtc
     3061 cggcgtaaac gtcattctga atttggtgcc accaggtgtc gaggcagtga agggctggcc
     3121 gttgaagaaa gttgcctgga tgtcgctcgg agccattggc tcagccgcta aggctgcgct
     3181 cgcagtgagg agaatccata cgacagtcaa tggtcctgta tcacgacgtt tcactcgatc
     3241 acctcgtcgg cgcggccaag aagcgtgatc gggaccgtaa ggcccagcgc cttggcggtc
     3301 ttcatgttga tgattagctc aaccttcgtc gcctgcagaa ccggcagatc ggccggtttc
     3361 tcgcccctca gaatccggcc ggcatagacg ccgatgtgtc ggtacatatc ccgaaaatct
     3421 tcaccatagc tcatcaagcc gccagctacg gcatactcgc gccctgcata aatggaagga
     3481 actgcgtggc ggagcagttg tgcgccgagt tgctcgatcc gggtggtgaa gaatccatcg
     3541 ctgccgatta cgagcgcgct tgcgcgcaag cttgcgagcg ctgtctcgat ctcgcgttca
     3601 gtgctggcgt gcacgacatg aagctgtacc cctagggtct gggccgccgc ttgcgtttgt
     3661 cttgatatgg tctcagcatt tgtattgatc ggatttacga gcagcgcaaa aatgttcgcc
     3721 gcagggacca cctcgcgcag tagctgcagc cgttttggtg ctacctcgac acccagcgag
     3781 gtcacccccg tgacattgcc acttggtcga ttgaggctgg cgacgaaacc actctggacc
     3841 gggtcgctcg cggtactgaa aatgatcgga atcgtcgtgg ttgcagcctt agccgcgcgc
     3901 accgaaaagt cgctgcccgt agcggcgatg acggcgaccg aacggccggt gagctcagca
     3961 gccagcgccg gaagtctttc gtaccgaccc tccgcccaac ggtactcgat gacaacattc
     4021 tggccctcga tatagccggc ttcgcgaagg ccctgctgga atgctgtctt attgcgcgcg
     4081 gtttcgaccg cggccggtgc ttgagcgttg agataaccga tgatcggaag cctcgcctgc
     4141 tgcgcgcgcg ccgcgagcgg caacgcagcc gcgccgccga gcagcgtgat gaactcgcgg
     4201 cgcctcatta ttcgattacc tcgtcggcag tagcgagcag cgtctgcgga acggtgatgc
     4261 cgagcatcgt ggcggtctgg acgttgaaga ccagctcgaa cttgctcgac tggaccaccg
     4321 gcaagtcggc gggcttggca cccttgacga tgcggccgac gtaaggaccg gcttggcgcc
     4381 atgcatccga aatgttggct ccataactca tcaacccgcc ggcttcgaca aattcccggc
     4441 cggcatatga tgccggaatc gcgtggcggg tcgcgaggtg gaccaattga gtccgtcggc
     4501 tgttaaagaa aggatcgaga ccgacgaaca gcgcgtcggg ccgctcacgc gtgaacgtag
     4561 caaaggccgc gttaatctcg tggatggtgc tggcgttgag cagctgtact tgcaatccca
     4621 tggtgcgagc tatcgtttca atttcgcgct cgttattctc cgtattggtc ggattgatca
     4681 gcacggccac acgagacgct gcgggcacca gctcacgcag gagtgcgagc cgtttagccg
     4741 tcaactcccc ggttaaaaaa ttgattcccg ttaggttgcc gcccggtcgg gcaagacttg
     4801 cgacgagccc gagcttgacc gggtctgcgg caatgacgaa gacgataggg atcgtcgtag
     4861 ttgctgtctt ggctgctaat accgtaggaa gagctccggt ggcgacgatc acgtcaactt
     4921 gccggtgaac cagatcacgc gccacttccg gcagtcgatc cgtctccgcc caacggtatt
     4981 caatcgcgac gttctgcccc tcgatgaggc ctgcctcctt aaggccttgc cgaaaggcgc
     5041 gaaggcgatc cgccaataca tctggcgatc tttgatcgag caatccgatc accggcatgg
     5101 tcgccgactg ctgcgcccgc gccgcgagcg gccacgccgc cgcgcagccg agcagcgtga
     5161 tgatgaactc gcgccgtttc attcgatcac ctcgtcggcc cgggcgagca gtgtcgccgg
     5221 cacttcgagg ccaagcgctt tcgcggcctt gaggttgatg gcgagccgat acttggtcga
     5281 tgcctgcacc ggcagatcgg cgggcttctc gcctctgagg atgcgatcga cgtagccggc
     5341 cgcgcgccgg tgcggctcga tgtcgtccgg cgcgtaggag atcagaccgc cgccagcgac
     5401 gaaatggtgt tggaagtaga ctgcggggag cctgtgccgg gctgccagcg cgaggatcag
     5461 atcgcgatga acaaaagccc acgaactccc cgtcacgatc aggccgccat tcggggtttg
     5521 cgtgaaggcc gtgatgccgc gctcgatctc gccggggtcg ctcacgtcga ggggacgcaa
     5581 ctccacgctg agcgtcgatg cgactgactg caagacggcg aactggccaa ccccctgagg
     5641 ggagttcgca tcccgaagca ccgccactct tgtcaccccc ggcgcgatct ccttgaggag
     5701 ctccagccat ttggcggtga ggctgtactc gtagctggtg aaaccggtgg cattgcggcc
     5761 cggctgcgcc aagctgtcga cccagccgcc gccaaccggg tcgatggcca gcgtgaacac
     5821 gatcggcacc gtccgtgtcg cctgcagcaa aggtccgacg accgtgccac cgctggccag
     5881 aatgacgtcc ggcgcgagtg cgaccaattc ggccgcatat cggcgcaggt ggtcggaatc
     5941 ggcggcccag cgggtctcga tgcgcacgtt gcgaccctcg gtccagccta attgctgcag
     6001 cccctgcacg aatgctgcga gacgggcgac tccttccgga gcgtctgtgg cgagtgcatt
     6061 aagtacgccg atggtccgca tccgctcgcc ctgctgcgcg cgcgccgcga gcggccacgc
     6121 ggccgcgccg ccgacgagca gagtgatgaa ctgtcggcgc ttcatcactc gatcacctcg
     6181 tcggcgcggg cgaggagcgc gtccggcacg ttcaacccaa gcttctttgc ggtctttatg
     6241 ttgatgacca gctcgaattt ggtcgggaat tgggcgggta aatcagctgg cttcgtcccg
     6301 cgtaagatcc gatcgacgta gctgcctgcc tgccgaaaaa gatggggttg gtcaattcca
     6361 tacgacataa gaccgccgtc taaaacaaaa aatcgggcga agtaaatcga gggcaaacga
     6421 agacgagtca ctagctcgat aatctccggc cgatggtctg aggtgaagct gttcggtggg
     6481 aaaatcaatc ctaggttggg ctttcctaaa gcgatgattt tctgttcgat ctctgactgg
     6541 ttgtgaatct caatggccgt caccgaaatg tttaaagtct gtgcggcctg ctcaatcgat
     6601 cgaaggtagg cagccgacca acttgaccaa cttgcgtcgc ctcccacgat aacaagtagc
     6661 gccgtgacgc tcggcgccat ctccttgagc aactgaggcc atttgcctcc cagcgaatag
     6721 tcggactgag taaagcccgt tacattgccg ccggggcgcg atacgcttgc gacgagccct
     6781 tcagcgaccg gatcaatact gagcaggaat acaacgggga tttcacgagt gcggtcggcc
     6841 acgaatcgtg ctgagtacgg gttcacagcc agaatcacgt ctggcgcgag tgatacaagc
     6901 tctcctgccg ccattcgaag atggtccagg ttgccgccgg ggacataacg aaataggaaa
     6961 cggaaactgc gcccctcgag ccaacccttt tcgcttaatc ctgcgacaaa gcccgcaaaa
     7021 ttcctctgcc atcccacatc gttttcagcg agtgccgtca aaacaccaat gctgcgcata
     7081 gatgcagctg tctgcgcgcg cgccgccagc ggccacgcgg ccgccgcgcc gccgagcagc
     7141 gtgatgaatt gccgccgtct catgtcggac atgggggcgt gcttcccccg cgctttgtgc
     7201 caacgaagcg tacccagggt atcacggagc ggccggcagg tccttgggca acacctgaaa
     7261 tgttctgaat cgacgcgtga ggtcgcgtcg cggtcgtgag gggcgctgga acgaggctga
     7321 gggccccgac tgggctaact ctagaggttc ccccgctgtg ggctagggtc ccgctgagtg
     7381 aacgacctca cacaaccagc cagccccggc gtggtatgct ctgatcggcg ttcagctcgg
     7441 ggcgaccagc atgggtcatc ttgacccgac ccaaaactct caagcggcat cggaaaagaa
     7501 gctcaaggcg tccgcgtgcg agctggagca gaagcttgag gcgcgcaccc gcgagctcgc
     7561 cgaggcgcgg gagcagcaga cggcgacatc cgaaattttg cgcgtcatct cgagctcgcc
     7621 gggtgacctg aagcccgtgt tcgaaaccat gctggagaac gcgacgaggc tcagcgaggc
     7681 caagttcggt tttttgttcc tgcgcgaagg ggatgcattt cgtgcggtcg cgatgcatgg
     7741 tccgccgcct attgtcgagt ggatgcagca ggcaccggtg gtcgagctac gcgatcaccc
     7801 acacgtcccg ttcgcccgca tcgcccgaac caaagaggcg ctccacgttt ccgacctcac
     7861 cactgagcag tcctatatcg agcgtgatcc ccgcatcgtc gccctcgtcg aagccgccgg
     7921 cgcgcgcagc tttatcgccg tgccgatgct caaggaggag caactcatcg gcgccatggc
     7981 gatctaccgc caggaggtgc gtccgttcac cgacaagcag atcgaattgg tccaaaattt
     8041 cgccaaccag gccgtcatcg cgatcgagaa cacgcggttg ctcaacgagc tgcgcgagtc
     8101 gctgcaacag cagacggcca ccgccgacgt gctcaaggtc atcagccgct cgacattcga
     8161 cctacaagca gtgttggaaa ccctggtgga gaacgcggtc cgcctctgcg acgcggatag
     8221 aggcatcatc aacagacagg acggggacct ttatcgcccc gcggccagct ttggcaaccc
     8281 gcccgaatgg atcgagatta tggaacggaa tccgatccgt aaggatcgaa cgtcggcggt
     8341 gggacgagcc attttggagc gccgcgtcgt gcacattcac gacattctcg cggatgccga
     8401 gtaccggtgg gccgaggaac atcgaggaga gaaggaaatg catcggacaa tcgtcgcggt
     8461 cccgatgctc agggagggtg ccgtcatcgg cgtgattgtg attcgc
//
LOCUS       DATLJT010000056         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3448530,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000056 DATLJT010000000
VERSION     DATLJT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448530"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VM910_01160"
     CDS             <1..438
                     /locus_tag="VM910_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVI61193.1"
                     /translation="PGHSGTELHGFLAYLVKCAVTDTPYVVFGYRGKQVRDNIHSFDL
                     VNAFWHFFEAPRLGEVYNMGGGRYCNCSMLEAIEKCQNFSGREMRWSYNEANRAGDHI
                     WWISDIRRFQAHYSGWSVRYDLDAILCEIFDAVNDRMSRSELG"
     gene            458..886
                     /locus_tag="VM910_01165"
     CDS             458..886
                     /locus_tag="VM910_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI61194.1"
                     /translation="MAEWEREGIADMLSIVIPAHNEEGHIADTVKGVAAALDAAGIRY
                     EILVVNDYSVDRTETIVRALGRNNPAVRCVNNDPPNGFGVAVRRGLADFRGDVVATFM
                     ADGSDTPADLVAFYRKLHEGYDCVFGSCFSRGGRTVAYPA"
     gene            1528..2475
                     /locus_tag="VM910_01170"
     CDS             1528..2475
                     /locus_tag="VM910_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HVI61195.1"
                     /translation="MRLLVTTGLLWALSTRVDLSHVKEVVDHVSLPLLAAGAAALLAT
                     SPFAALRWHIVLAAGTTSPGPWTLLKIVLVGLFFNQVLPSGVGGDAVRAWRCHRLGIG
                     VAAAIRSLVLDRVSGYVVMVVLFAAGLPVLLHILPDVRQRYGVVLLLGAALCGLLALF
                     LIDYLPWGLLRFRLVAELAGLSREGRRLFGRPARAGALTGLSVATVGLTIVASMLVAE
                     SLGVDLSFFRWVVIIPPVTLIQLVPVSLAGWGVRELGFVVVLAGFGIPAEAALAASLL
                     FGMCMIVVGLPGGLLWLTGWDIASPTAASNGRVAAQADP"
BASE COUNT          471 a          794 c          810 g          621 t
ORIGIN      
        1 cccggccatt ccgggaccga actgcacggg tttctcgcct accttgtgaa atgtgcggtg
       61 accgataccc cttatgtcgt gttcggctat cgcggcaaac aggtgcgcga taacatccat
      121 tcctttgacc tggtgaacgc gttctggcat ttcttcgagg ctcctcgcct gggtgaggtt
      181 tacaacatgg gtggcggccg ctactgcaat tgctcgatgc tcgaggcgat cgagaaatgt
      241 cagaacttct ctggccgaga aatgcgatgg tcctacaacg aggccaaccg tgccggcgac
      301 cacatctggt ggatcagcga cattcgccgg ttccaagcgc attactccgg ctggtcggtg
      361 cgctacgacc tcgatgcaat tctttgcgag atattcgacg cggtaaatga ccggatgtcc
      421 cggtcggagc tgggttgatg ccgctcgcgg tcaatccttg gccgaatggg agcgggaagg
      481 catcgctgac atgctctcca tcgtgatccc ggcccataac gaggaaggtc atatcgccga
      541 tacggtgaag ggagttgcgg cggcgctcga tgcagctggg atccgatacg agatcctggt
      601 ggtcaacgac tacagcgtcg atcggacgga gacgatcgtc cgtgccctcg gtcggaacaa
      661 tcctgcggtg cggtgcgtca acaacgaccc accgaacggc ttcggcgttg ccgtgcgacg
      721 gggcctcgcc gatttccggg gcgatgtcgt agcgaccttc atggcggacg gttcggatac
      781 gccggccgat ctcgtcgctt tctaccgcaa gctccacgaa ggctatgact gcgtgtttgg
      841 ctcgtgcttt agccgaggtg ggcgcacagt ggcctatccc gcctaaagcc agtattgaac
      901 cgattgggaa acaaattcat ccagctactg tttcgcattc cccgcgatga catttcgaat
      961 gcgtccaaaa tgtatcgacg cactgtaatc gccggtgtcc agccgctgct cgcccagcac
     1021 ttcaatttga cagtcgagct cccgctcaag tcgatctgcg cggctaccgg tatgcgatcg
     1081 taccgaatac ctggatcaat cgcaatcagg gtgtgtcgaa gttcaaaatc aaggaaatgg
     1141 gatcgctatt ttttcatcgt gctctatagg ctcctcgaaa agactctggg gcacgcagat
     1201 ctgcggacat ccacgcagtt gcgcgagacg cagctccagg tgtggcaccg ttgagaccgg
     1261 agtgagggca tcgcccgtca gcaatgatag ctcctaatgg agaaattttg gctttccata
     1321 attattccat gcaataacgg cgcgcgttgg ctcatagccg cgctccgaag atcggtggag
     1381 cagaacgcac cttacatctc ggcttccggc gcgatttgtt cgcgggttct gaaacccccc
     1441 ttaacttcaa acggacttgc gcttttgcta gcacacagcc acccaacgaa gcacgtaact
     1501 gcgatccgca agcttctgat ccccagcttg cgtctgctgg tgaccaccgg gctattgtgg
     1561 gcgttgtcga cgcgcgtcga tctaagccat gtcaaggagg tcgtcgatca cgtctcgttg
     1621 ccgctcttgg cggcaggcgc ggcagcacta ctggcgacga gcccgttcgc cgcgctgcgc
     1681 tggcatatcg tcttggcggc cgggacgacc tctcccggac catggacctt gctgaagatc
     1741 gtgctcgtcg ggctgttctt taaccaagtc ctgccttccg gcgtcggcgg cgacgcggtt
     1801 cgggcgtggc gttgccaccg gctcggcatc ggcgtggctg cggccatccg cagcctcgtg
     1861 ctcgatcgtg tcagtggcta cgttgtgatg gtcgtcctct ttgcggcggg cctgcccgtt
     1921 ctgctgcaca ttctccctga cgtacgccaa cgctatggag tcgtcctgct gcttggggcg
     1981 gcgctatgcg gattgctcgc gctgttcctg attgattacc tgccgtgggg gctattgcgt
     2041 tttcggctgg ttgccgagct tgctgggcta tcgcgcgaag ggcgtcggct gtttggtcgg
     2101 ccggcgcggg ccggagctct cacgggcctt tctgtcgcca ccgtggggct tacgatcgtt
     2161 gcctccatgc tagtggcgga gagtctcggc gtcgatcttt ccttctttcg ctgggtcgta
     2221 atcatacccc cggtcacttt gatccagctg gtacccgtgt cgcttgccgg gtggggggtg
     2281 cgcgaactcg gctttgtcgt cgtcctcgcc gggttcggaa tcccggccga ggcggcgctc
     2341 gctgcctcgc tgttgttcgg catgtgcatg atcgtcgtcg gtttgcccgg cggtttgcta
     2401 tggctcaccg gctgggacat cgcatcgccg accgctgcga gcaacggacg tgttgccgcc
     2461 caggccgatc cataataaac aatgcgtcta taaattacag tcaaaccgga cccaatcagg
     2521 ccttctgatt ggacgggagt tcgtggacga caactaagac ggcagggtag ggccgcctct
     2581 ccctttccct gccgcgcagg gaaactcagg gtaaaaattt ttttgaggtt cgcccggtat
     2641 cgaaagtcgt ttctgatcaa aaccttgcac gcgactcttc ggtcgaatcg aaggcg
//
LOCUS       DATLJT010000057         5943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1840194,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000057 DATLJT010000000
VERSION     DATLJT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840194"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VM910_01175"
     CDS             <1..403
                     /locus_tag="VM910_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61196.1"
                     /translation="SEEVICALLRDFREHGQKAIAKVRREQPGVYLRCLTLLVPREHK
                     AEHSNAIKDMTDEQIERSIEVIEQILAEREAGANAKVIEGEAEVVPSLPAPDVVPDGP
                     NKVMDAADTAVGARERKPRKRKVPSPASVIS"
     gene            1269..1613
                     /locus_tag="VM910_01180"
     CDS             1269..1613
                     /locus_tag="VM910_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61197.1"
                     /translation="MKRHAKPERAAFLDMQVVALSAGGDGGSGVLEQSKDLFGMPPSG
                     LTIRCDCTPSSGAREISTTILGAIRLDGPSATREVIEVREALEETKAHGIRFKSAKPK
                     GRPAQDHPAEYC"
     gene            1657..1968
                     /locus_tag="VM910_01185"
     CDS             1657..1968
                     /locus_tag="VM910_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI61198.1"
                     /translation="MLLGQGRLAPDDLLFGNLEGGPLRPSAISSDWGDVAERLGMPEV
                     TFHGLRHTHASQLIAGNVDIVTISKRLGHAKPSVTLAIYAHMFHTDDSKAAVAINAAL
                     G"
     gene            2037..2129
                     /locus_tag="VM910_01190"
     tRNA            2037..2129
                     /locus_tag="VM910_01190"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2071..2073,aa:Ser,seq:gct)
     gene            2270..3532
                     /locus_tag="VM910_01195"
     CDS             2270..3532
                     /locus_tag="VM910_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI61199.1"
                     /translation="MARTIGRLTALKVDKAKRPGMYADGGGLYLRVTHNGTKNWVFRF
                     MLNGRPRWMGVGPLHIIGLAEARNRAAAFRLQRHDGIDPIERRRADRLQGRLEAAKAI
                     TFNECAARYIASHRAGWRNPKHAAQWQATLATYAERVIGGLSVQAIDTALVHRVLEPI
                     WTTKPETAGRVRGRIESILDWAKVRGYRAGENPARWRGHLDKLLPVRSKVRRVEHHAA
                     LPYAELPGFLVSLREQEGIGARALEFAIVTAARTGEVIGARWGEIDLLDKTWTLPATR
                     MKAGKEHRVPLSGRALAILQDMQAHRHADEAFVFPGAKPGRPLSNMAFLMLLRRMARG
                     YVTAHGFRSSFRDWVAERTNFPAEVAEIALAHTVSDKTVAAYNRSDLFDRRRRLMADW
                     ATFCTAPEQKVQSNVALLHQGPASNPRG"
     gene            3590..4303
                     /locus_tag="VM910_01200"
     CDS             3590..4303
                     /locus_tag="VM910_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61200.1"
                     /translation="MTMAGIASPFSEHAVELIKASLPGGIDQRRLDLLPLILNEWSRT
                     DLREHLSRDTPAASRKRFAQLTKVGKCATHLRQALEALDQRGWVWIAQEIGREDGSPL
                     WSVTREGLSEMKERLKQQDDFLKRIEAAIPRLLEEDKQESGHPRNIRAYLVVMDLVAI
                     FEWLTGRKATREVDRDLGKDTGPFWDFAAAVWPHVFGQGGYGLSAAMKNWKKWHSLYG
                     EESPFIRNMAMRHPEMADI"
     gene            4751..>5943
                     /locus_tag="VM910_01205"
     CDS             4751..>5943
                     /locus_tag="VM910_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61201.1"
                     /translation="MRDGTIQGAAEIIDLALKPKPELIVHAGNLPATADGLRDLLAAS
                     GKLFDRGLPVRVIRPADGTLPSAVRLTKHNVVMEAHRLCQPVKIDADGERVPVTLPDR
                     VAQMYLDMCGEWHLSPLAGVSTAPLLSQDGSVREADGYDPETGLWCCGVPRLKLPVRP
                     SRGEAEAALMQLRNAFRTFPFADAPRCRDASLAVEIIDVSCPPGRDESAFLNALLTAV
                     CRASLWLAPGLLLTAPAVSGAGTGKGLLVRAICTIAFGIRPRAFTMGSDRQELDKRLA
                     AELIEAQPALFLDNANGVVLRSDTLASVLTERPARVRVLGQTRMVPLNSTAFVAITGN
                     GLTVTEDLARRFNHCQLDAHCEDPELRQFGSGFLDQIERRRDELLVACLTIWRWGRQN
                     ATLLPR"
BASE COUNT         1185 a         1771 c         1878 g         1109 t
ORIGIN      
        1 gagcgaggaa gtcatttgcg cgcttctacg cgatttccgc gagcacgggc aaaaggccat
       61 tgcgaaggtg cgccgcgagc aacccggggt gtacttgcga tgtctcaccc tgcttgtccc
      121 gcgcgaacac aaggccgagc atagcaatgc gatcaaggac atgaccgacg agcagatcga
      181 gcggtccatc gaggttatcg agcaaatact agccgaacgg gaagccggcg cgaatgccaa
      241 agtgattgag ggggaagcgg aagtcgtccc gagcttgccg gcgcctgacg ttgtcccgga
      301 cggaccgaac aaggtcatgg atgcggcgga caccgcggtg ggggcgaggg agcgcaagcc
      361 gaggaagcgc aaggtgccat cccctgcgag cgtaatttca tgatgtcggc ggcttagacg
      421 ccttcggcac gcgattgcag acggcgagcg caattccgat ctcttgtgcg aaggcgcaat
      481 cgagaaatta cgtgagcatt tgttcggaga ttaacaggga ccgccaagcg ccccgggtcc
      541 gatgattgca aaaatcctca ggaccaggtt gtaaggaact tctggcgcgc ccgcatattg
      601 cccaagcgga cccctagcca aaaagccatg gggttgccga gaccccgccc gacctccctt
      661 tggcaatgcc acactcgggc ggggttttcg tcagacaaac ccgccgagac gctcgcgtcg
      721 cgcagcaacg gcggcgactg gggaagaagt ttacctggtc atggtggaac gttggcgttt
      781 acgcgggcgt tacgtcgcgg ggtcgactcg tcaaaaaggg aggtaaacga tgaaacgtgc
      841 tcttatcaca atcgcaactg tgggcatttt ggctccggcc gcctgggtcg ggaccgcaaa
      901 cgcacaatat ggcgctccgg ccccaggcta cgcccctgca ccagcctatg cccctgcgcc
      961 agcctatggc ccttctcgac gatatgcccg tcgagcctac cgtcgctctg ctcgtcagac
     1021 ctatcgtggt gctccccctg cggtctatgc cggtggtacg gccttcaccc ctggctctgg
     1081 tgggcagccc tatgccgtta ctcctcgaac atatgccgca caatctgccc aaccgaatga
     1141 atggtacggc ccaatgagac gcggtaatat gtgcacgacc cacgtcgata tcaatcgcgg
     1201 ttacgcctac gaaacggcgt gcaagaagtg agtgtgtgac aagagccccg cccgagcggg
     1261 gcttttcttt gaagcgacac gcgaagccgg agcgggcggc cttcctcgac atgcaagtgg
     1321 tggccctctc ggctggtggc gacggcggca gcggagtgct tgagcaaagc aaagacttgt
     1381 tcggcatgcc accgagcggc ctcaccatcc gctgcgattg cacgccgagc agcggcgcac
     1441 gagaaatttc aacgacgata cttggggcga tccgactgga tggcccatcc gctacccgcg
     1501 aggtcatcga ggtgcgcgag gcactcgagg aaacaaaggc tcacggcatc cgtttcaagt
     1561 cggcgaagcc taaaggccgg ccggcgcaag atcaccctgc cgaatattgc tagcgaggcg
     1621 ctacgagaac accgcaagca acttttggag acgcgattgt tgctggggca ggggaggctt
     1681 gccccggacg atcttctgtt cggtaatctc gagggcggcc cgcttcgccc aagcgccatc
     1741 tcatctgatt ggggcgatgt cgctgagcgc cttggtatgc ccgaggtcac gtttcatggg
     1801 ttgcgccaca ctcacgcctc gcaactgatc gcgggcaatg tcgatattgt caccatcagc
     1861 aagcgcctgg ggcacgccaa gccgagcgtc acacttgcga tctatgccca catgttccat
     1921 accgatgaca gcaaggccgc ggtggcaatc aacgcggccc ttgggtagca aagcggtagc
     1981 aaagttcaca tttgttctgt cggcacccat gcttaagtgc ttgaaactac aacgccggag
     2041 acgtggccga gtggctgaag gcggcggttt gctaaaccgt tatacggtct aaagccgtat
     2101 cgagggttcg aatccctccg tctccgccat ctcggcattc gccgccgttc gctgacgttc
     2161 agaaatctgc agaaacggcc aatctgccgg cctctttcgg ctttacacgt tcgcagccgt
     2221 tcgccacaat ccggaggatg attgtggtct gattcgtgga ccgggtggca tggcgcgaac
     2281 gattgggcgg ctgacggcgc tcaaggtgga taaggcgaag cggccaggca tgtatgccga
     2341 cggcggcggt ctctaccttc gcgtgacgca taacggcacg aagaactggg ttttccggtt
     2401 catgctgaac ggtcggccgc gctggatggg ggtcggaccg ctgcacatca tcgggctcgc
     2461 tgaggcacgt aatagagcgg ctgctttccg ccttcagcgc cacgacggga ttgacccaat
     2521 cgagaggcga cgtgctgata gattgcaagg gcgcctcgag gccgcgaagg cgataacgtt
     2581 caacgagtgt gccgcaaggt acatcgccag ccatagagct ggctggcgca accccaagca
     2641 tgcggcgcaa tggcaagcga cgctcgccac ctatgccgag cgcgtcattg gcggtctctc
     2701 ggttcaggcc atcgataccg cgctcgtgca cagggtcctg gagccaatct ggacgacgaa
     2761 gcccgagact gcgggccgcg tccgcgggcg tatcgagtcc atcctggatt gggcgaaggt
     2821 acgcggctat cgcgcgggcg agaacccggc ccgatggcgc gggcacctcg ataagctgtt
     2881 gccggtgcgc tccaaggtga ggagggtgga gcaccatgcg gcgctgccct acgccgagtt
     2941 gccgggcttc ctggtgagct tgcgcgagca ggagggcatc ggggcgcgcg cactcgagtt
     3001 tgccatcgtg accgccgcta gaaccggcga ggtgattggc gcgcgatggg gcgaaataga
     3061 cttgctcgat aagacttgga cgctccctgc tacgcgaatg aaggccggta aagaacaccg
     3121 cgtcccgcta tccggtcgcg cgctcgccat cttgcaggac atgcaggcgc atcgtcatgc
     3181 cgacgaggcc ttcgtgttcc cgggcgccaa gcccggtcga ccgctctcga acatggcgtt
     3241 cctgatgctg ttacggcgga tggctcgggg ctatgtgacg gcgcacggat ttcggtcgag
     3301 cttccgcgat tgggtggctg agcgcacgaa cttccctgcc gaagtagcgg agatcgcgct
     3361 agcgcacact gttagcgaca agacggtggc ggcgtacaat cgatccgacc tgttcgatcg
     3421 ccgcaggcgt ctgatggcag actgggcgac cttttgcacc gcgcccgagc aaaaggtgca
     3481 aagcaatgtc gcgttgctgc accaaggacc agcatcaaat cccaggggct agggcggtca
     3541 acagtgccgc gctgccgccg cgccttgcgg agtctcagtg gtggcacaca tgacgatggc
     3601 cggtatagct tccccattca gcgagcatgc cgtagagctg atcaaggctt ccttgcctgg
     3661 agggatcgat cagcgacggc tcgatttgtt gccgctgatc cttaatgagt ggagccgcac
     3721 cgatttacgc gagcatttgt cgcgagacac tcccgcggcc agtcgaaagc gtttcgccca
     3781 gctaaccaag gtcggaaagt gcgcaactca cctacggcag gcgcttgaag cgcttgacca
     3841 acgtggctgg gtctggattg cacaagagat cgggcgcgaa gacgggagcc cactgtggag
     3901 tgtgacccgc gagggactct ccgagatgaa ggagcggctc aaacaacagg atgattttct
     3961 caagaggatt gaggctgcga taccgaggct ccttgaggag gataagcaag agtcaggaca
     4021 cccccgtaat atacgtgcat acctggtcgt gatggacctc gttgcgattt tcgagtggct
     4081 tactggcagg aaagcgaccc gagaggtgga ccgcgatctt ggtaaggaca ctggcccatt
     4141 ctgggatttt gccgcagcgg tgtggccgca tgtgttcggg caaggcggtt atggcttgtc
     4201 tgcggcaatg aagaactgga agaaatggca ttcgctctat ggcgaagaat ctccgttcat
     4261 taggaacatg gctatgaggc atccggagat ggcggatatt taacgctggc tccacaatag
     4321 tccccatgtg ccaaagttag cgttcgtccc catctccgcg ttcgaccgta ggcgcacgcg
     4381 gagaaccatc gtgagcgtgt taagcacggg cctttcagat tacgaaactc gtggcgcgaa
     4441 gccgctcact gtgaccgtgg gtaccgccaa aaagctcagc gggctcggca ataccacaat
     4501 ctgggcactg atcaaagatc gaaaactcga aacggtgcac gtcggtcgcc gcacgcttat
     4561 cacttatcgt tcacttgaag cattgcttgc gcctcgctca cagccgcggc cgcggcgggg
     4621 tcggccgcgc aagaacctcc ccagcggcgg agaagcaccg tgatggccgc agacatcgac
     4681 ccgtttggca cccccgcgag gctcaagcga accgcgtggc gcggcatctg ctcaactgga
     4741 gggctcgccc atgcgtgacg ggaccattca gggcgccgct gagattattg atctcgcgct
     4801 caaacccaaa ccggaactta tcgtgcatgc gggcaatctg ccggctaccg cggacgggct
     4861 gcgcgatttg ctcgcggcgt ccggaaagct attcgaccgc ggccttcctg tgcgcgtcat
     4921 cagacctgcg gatggcacct tgccatcagc cgtgcggcta acgaagcata atgtcgtcat
     4981 ggaggcgcac cggctttgcc aaccggtgaa gatagacgcc gacggcgagc gcgtcccagt
     5041 aacgttgccg gaccgcgtcg ctcagatgta tctcgacatg tgcggcgaat ggcacctgtc
     5101 cccgttggct ggtgtcagca cggcaccgct gctatcccag gacgggagcg tgcgcgaggc
     5161 tgatggctac gatcctgaga ctggcctttg gtgctgcggt gtcccaagat tgaaactccc
     5221 ggtgcggccg tcgcgtggcg aagccgaggc agcgctgatg caactgcgca acgcgtttcg
     5281 gactttcccg ttcgcggatg cgccgcggtg ccgggatgcg tccctagcag tcgaaatcat
     5341 tgatgtctcc tgcccgccag ggcgggatga aagcgcgttt ctgaacgccc ttctgacagc
     5401 ggtctgtcgc gccagcctgt ggcttgcgcc gggattgctg ctcaccgcgc cggccgtctc
     5461 gggcgcgggc actggcaagg gacttcttgt gcgcgcgatc tgcaccatcg cattcggaat
     5521 ccgaccgcgc gcattcacaa tgggaagcga ccggcaagaa ctggacaagc gacttgcagc
     5581 ggaactgatc gaagctcagc ccgccctgtt tctggacaac gccaacggag tagtgctgcg
     5641 ctcggacacc cttgcttctg tgcttaccga gcgtccggca cgggtccgtg tgcttggaca
     5701 gacacgaatg gtgccgctga acagcacggc attcgttgcg attacgggaa atggcctgac
     5761 cgtcaccgaa gacctggccc gccgcttcaa tcactgccaa ctcgatgccc attgcgagga
     5821 ccccgagctg cgccagttcg gctcgggttt tcttgaccag attgaacgcc gacgcgatga
     5881 actcttggtc gcatgtctca cgatctggcg ctggggccgt cagaacgcga cgttgctacc
     5941 acg
//
LOCUS       DATLJT010000058         3948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1840217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000058 DATLJT010000000
VERSION     DATLJT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VM910_01210"
     CDS             complement(<1..387)
                     /locus_tag="VM910_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61202.1"
                     /translation="MIHRPHFGRFRTGLLAATAVCLAASAHAQSGEPIRIGVIAEVQS
                     IAGAATPGGAQIAADEINAKGGILGRKIEIVTYDNKSSSADSVRAFQRAVSEDKVSAV
                     IASYISEVVLALEPWAARLKMPLITPG"
     gene            complement(537..2489)
                     /locus_tag="VM910_01215"
     CDS             complement(537..2489)
                     /locus_tag="VM910_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61203.1"
                     /translation="MIRFPFRRTLPGIMFAGALALLPAQHAVAQRAVDTPLQISWEVK
                     NRFRLFREERDFQLHVESARDRNVLASEQVLELQSDGRGWARNVVNRLCIDLQGRVSE
                     PCTRDNVKESYLTPIDHAIVVRLVGAVPVGATCAWTFDDGDGPQQSTFDCAEPVNLRV
                     RYGRQTVATVDVASGQEAPQRISTEIQVRDIFIAGLGDSIASGEGNPDKPIGLSDEGF
                     CFRYYLGSAAAQYYRPSRAGYKGGRACEAPDSLQNWQRYGALWFNAACHRSLYSYQTR
                     TALALAVQYPHIAVTYLPLACTGATIADGLFGSQRARECPPSKSTSNCQASVNGQLAE
                     LREAVIAARRRQPDRQLDLVLLSIGANDIHFSGLVADVIVDTPTERTLFRRTGVLATL
                     DDSRAALARDLPQSFLKLREALKPLVGDLSHVIYTAYANPALSNGAPCPGGRAGFDIH
                     PSFNADPQRLASVTSYVETEFLPELKALALCQSGIICRDPAADRMTFVDAHQATFADH
                     GFCARAQNDPEFDRECFSLKGESFDPDIVNSATEPMLCGRSASEYRAYLPRARWIRDA
                     NDSYFAAMTYPQGLPSAMQPSDIHDATWGVLSAVYGGAVHPSAEGHAVMADAVLPAAA
                     AVLQLNAVVGDVSSEPAVPLTAPEQR"
     gene            complement(2721..3836)
                     /locus_tag="VM910_01220"
     CDS             complement(2721..3836)
                     /locus_tag="VM910_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HVI61204.1"
                     /translation="MQGLLTTLARGFKKWIGWKRLGVAASLFIIAFAITTLVRTLKGV
                     DTGVILTALTEIRPGHIALAALCVVCAFCTLTFYDFFALRTIGKRHVPYRIAALSSFT
                     SYSIGHNIGATVFTGGAIRFRIYSEYGLNAIDVAKICFLSGLTFWLGNLLVLGVGMAY
                     HPWAASAMDLLPAAMNRLIGIGCLAGIVMYLVWLVLGENRRELGQHGWKVVLPSARLT
                     LVQILIGVVDLGFCATAMYLLMPAQPDIDFLSLAVVFILATLLGFASHAPGSIGVFDA
                     AMLVALPQFGREQLLATLLVFRVLYFVIPFTIAISIMGTREFWLNVVAPWQKRRKMNG
                     NGNGNCAPSAAARPTKTTPAQTAPIRPKPVKRLRSQA"
BASE COUNT          747 a         1215 c         1296 g          690 t
ORIGIN      
        1 gcccggcgtg atcagcggca tcttgagccg cgccgcccac ggttccagtg cgagcaccac
       61 ctcgctgata tagcttgcga tcaccgcgct caccttgtct tcgctgaccg cgcgctggaa
      121 cgcacgcacg gaatccgccg acgagctctt gttgtcatac gtcacgatct cgatcttgcg
      181 gcccaggatg ccgcccttgg cgttgatctc gtcggctgcg atttgtgcgc ctccgggcgt
      241 cgcggcgcct gcgatcgact gcacttccgc gatcacgccg atcctgatcg gctcgccgga
      301 ttgtgcgtgg gcggaagccg cgaggcagac cgcagtggcg gcaagcaggc cggtgcggaa
      361 ccgcccgaag tggggacgat ggatcatctt gttgtctctc ccttgatgcg ttttgcgttg
      421 tcatggtgac ggcgccggcg aacgacggcg cttcaacgtt agcctacgcc gcgccggtgc
      481 ggcttgagaa cacgcgtttg aaactgcctc cttgcgagag gcgcatggcg gaaggctcag
      541 cgctgctccg gcgccgtgag gggaacggcg ggttcagaac tcacatcgcc cacgaccgcg
      601 ttgagctgaa gcacagccgc tgccgccggc agcactgcat cggccatcac ggcgtggcct
      661 tcggcggaag gatgaacggc gccgccgtag accgcagaca agacgcccca ggtcgcgtcg
      721 tgaatgtcgg atggttgcat cgcggaaggc aacccctgcg gataagtcat ggccgcgaaa
      781 tagctgtcat tggcgtcgcg gatccagcgc gcccgcggca gataggcgcg atattcggag
      841 gcgctgcggc cgcacagcat cggttcggtc gcggagttga cgatgtcagg gtcaaagctc
      901 tcgcccttca gcgagaagca ttcgcggtca aattcgggat cgttttgggc gcgcgcgcag
      961 aagccatggt cggcgaatgt agcctgatgg gcgtcgacga atgtcatgcg gtcggcggcg
     1021 ggatcgcggc agattatgcc ggactggcac agcgcgagcg ccttcagttc cggcaggaat
     1081 tcggtctcca cgtagctcgt gaccgaagcg agccgctgcg gatcggcgtt gaacgaggga
     1141 tggatgtcga agccggcgcg tccgcccggg cacggcgcgc cgttcgacag tgcaggattg
     1201 gcataggcgg tgtagatgac atgcgagagg tcgccaacca gcggcttcaa tgcctcgcgc
     1261 agcttcagga agctttgcgg caggtcgcgc gccagcgctg cgcgcgagtc gtccagcgtt
     1321 gccagcacgc cggtgcgccg gaacagcgtg cgctcggtcg gcgtgtcgac gatgacgtcg
     1381 gcaacgagcc cggaaaaatg gatatcgttg gcgccgatcg acagcagcac gaggtcgagc
     1441 tggcggtcgg gttggcggcg ccgggcggcg atcaccgcct cgcgcaattc ggcaagctgc
     1501 ccgttgactg atgcctgaca gttcgacgtc gatttgctcg gcgggcactc gcgcgcgcgt
     1561 tggctcccga acaatccgtc ggctatcgtg gcgccggtgc aggcgagcgg cagataggtc
     1621 accgcgatgt gcgggtattg tacggcaagc gcgagtgcgg ttcgggtctg gtagctgtag
     1681 agcgagcggt ggcaggcggc gttgaaccag agcgcgccgt agcgttgcca gttctgcaaa
     1741 ctatcgggcg cttcgcaggc gcgcccgccc ttatagccgg cccggctcgg ccggtagtat
     1801 tgagctgcgg ccgaacccag ataatagcgg aagcagaagc cttcgtcgga taggccgatg
     1861 ggtttgtccg gattgccctc gccggaggcg atgctgtccc caagccccgc gatgaagatg
     1921 tcgcgcacct ggatctcggt tgagatcctc tggggcgctt cctgtccgct ggcgacgtcg
     1981 acggtggcaa ccgtctggcg gccgtagcgg acgcgcagat tgaccggttc ggcacaatcg
     2041 aaggtcgatt gctgcggacc gtcgccgtca tcgaacgtcc aggcgcaagt ggcgccaacc
     2101 ggcactgcac cgaccagccg aacaacgatc gcatggtcga tcggcgtgag atagctttcc
     2161 ttgacgttgt cccgcgtgca gggctcgctg acgcggccct gcagatcaat gcaaagccgg
     2221 ttgaccacgt tgcgcgccca gccgcggccg tcgctttgca attccagcac ctgctcggaa
     2281 gccagaacat tgcgatcgcg cgcgctttcg acgtggagct ggaaatcgcg ctcctcgcgg
     2341 aacagacgga agcggttctt gacctcccaa gaaatctgca gcggcgtgtc gacagccctc
     2401 tgcgcgaccg catgttgggc cggcaacagt gccagcgctc ccgcaaacat gatgcccggg
     2461 agagtccggc ggaaggggaa acggatcatg gcgcggtttt gacgttacag ttggggcgga
     2521 aataagagta tccgtccgaa accaatggcg cgattcgacc attcgcattc ccttcgggag
     2581 gcacgcttgc gaatgtgaaa atcgaaggac cgccagcaaa tcattattcc gatggcgctt
     2641 tgttttgacg ttcgcgtgag ggaaccgcgg gcaaatcggc ggtgagcgtc aaatccgctc
     2701 cggcgggacg acgtaacctg tcacgcctgc gatctgaggc gtttgaccgg cttgggtcgg
     2761 atcggggcag tttgtgctgg cgtcgtcttc gtgggccggg ccgctgcgga tggcgcgcaa
     2821 ttgccgttcc cgtttccgtt catcttgcgg cgcttttgcc agggcgccac gacgttgagc
     2881 cagaactccc gtgttcccat gatggaaatg gcgatcgtaa acgggatcac gaaatacagc
     2941 acgcggaaca ccagcagcgt cgcaagcagt tgctcccggc cgaactgcgg cagcgccacc
     3001 agcatcgccg cgtcgaacac gccgatgctg cccggcgcat ggctggcgaa gcccaacaac
     3061 gtcgccagga tgaacaccac cgccagcgac agaaaatcga tatcgggttg ggccggcatc
     3121 aacagataca tcgctgtggc gcaaaatccg agatcgacca cgccgatcag gatctgcacc
     3181 agcgtcagcc gcgccgacgg caacaccact ttccagccgt gctgtccgag ttcgcgccgg
     3241 ttctcaccga gcacgagcca gacaagatac atcacgatcc cggccaggca gccgatcccg
     3301 atcaggcggt tcatcgccgc cggcaacaga tccatcgcgg aggccgccca tggatggtag
     3361 gccatgccga ccccgagcac gagcaggttg ccgagccaga atgtcagccc ggacagaaag
     3421 cagatctttg caacatcgat cgcgttcagc ccgtattccg aatagatccg gaaacgaatg
     3481 gcgccgccgg tgaaaacggt ggcgccgata ttgtggccga tcgaatagct cgtgaagctc
     3541 gacaacgctg cgatgcggta aggcacgtgc ctcttgccga tcgtgcgcaa ggcgaagaag
     3601 tcatagaacg tcagcgtgca gaacgcgcag accacgcaca gtgcggcgag cgcaatgtga
     3661 cccggccgga tttcggtcag cgcggtcagg atcacgccgg tgtcgacgcc cttcagcgtg
     3721 cggaccaggg tggtgatcgc aaaggcgatg atgaacaggc tcgccgcgac gccaagtcgc
     3781 ttccagccga tccatttctt gaaaccacgc gccagcgtgg ttaacagtcc ttgcattcat
     3841 cctcccggca gggcgatgag gtcttgaaac gaccttcaaa ccatcgatcg aacacggcag
     3901 ccgatcgcac ggtatgcccg agttccttaa ggcaaaagca ccgccttg
//
LOCUS       DATLJT010000059         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2529762,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000059 DATLJT010000000
VERSION     DATLJT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529762"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VM910_01225"
     CDS             complement(<1..555)
                     /locus_tag="VM910_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HVI61205.1"
                     /translation="MPPAVLYLKNRLAAADRRYAGRFALLAAIHLAAFGVLLWSEDEP
                     AARAAFVLTWGALNFFWLVLLRRPLTSAALSFALIVILIALSQFKHGTLMMTATFVDV
                     MLIDVATFWFFLKVNPGLVGKLALAAALAMPVMVLLWRTEPFRVRRGTALLGCLLCLI
                     ALAGLSLAVPTDREDEFYPHQYVSK"
     gene            1427..>2070
                     /locus_tag="VM910_01230"
     CDS             1427..>2070
                     /locus_tag="VM910_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HVI61206.1"
                     /translation="MSAAHAAPKTCTGLWDFIETDCQLTWYGITLYGVIDAGFTYQTH
                     GAPLDPRSPPGSAYIVQRYSRSSRWDLAPNGLQNSFVGIKGTEPIGGNTSVVFALDAG
                     FDPYSFKLSNGPGSAAHNAGVPQDQQTSWADSSRAGQWYNGNGYVGLSSPSYGTLTVF
                     RQNVLTYDGVLEYDPMGASYAFSPIGWQGLTCGGGNTENCRHTTSLKYRLTVGQA"
BASE COUNT          427 a          604 c          661 g          378 t
ORIGIN      
        1 tttggagacg tattgatgcg gatagaattc gtcctcgcgg tcggtcggca ccgccaacga
       61 cagcccggcg agcgcgatga ggcaaaggag gcatccgagc aaggcggtgc cgcggcgcac
      121 ccggaacggc tcggtccgcc acagcaggac catcacgggc atcgccaacg ccgcggcgag
      181 cgcgagtttc ccgaccagac ccggattgac ctttagaaaa aaccagaacg tggcgacgtc
      241 gatgagcatc acgtcgacga aggtcgccgt catcatgagg gtgccgtgct tgaactgcga
      301 cagcgcgatc aggatgacga tcagcgcgaa cgacagcgcc gccgaggtca gcgggcgccg
      361 cagcaggacg agccagaaaa agttgagcgc gccccaggtg aggacgaacg ccgcgcgcgc
      421 cgccggctcg tcctcggacc agagcaacac gccgaaggcg gcgagatgga tggcggcaag
      481 gagcgcgaag cggccggcat accggcgatc ggcggccgcg aggcggttct tcaggtagag
      541 gacggcgggc ggcatgattg acggctgcag ccgcgctctg gtcgacattc accgcggcgg
      601 gcggctgtca tctttctgta ataaaattgt catggcaagg ggctggagcc gccgggcgcg
      661 tcgcgcggac ttgattttaa tccgattcag accggatggg ccttacggag gcgcatttga
      721 aattagatcg gcaggcaatg gacgcactgc gcaagagtaa agcgcccgtc gcgcctcgat
      781 aacatgcggt cgtttaggcg ccgctatacc actgccaaag ggggtatccc cgccagtgac
      841 agaagtcggt ttgtccggac cgacaaagtt gttcatcagt tgggaaaacg aactcggttt
      901 gcgtggaagg ccgcgcggcg ggccgagcga aggtcgggct ggcgaacagc gtgaccaacg
      961 cgagagcagc gataagtggc ttcatcatat ctctccatgc ttatggacca ccgttggtga
     1021 ttttcctgcg acagaagaag agcgacgcct cgtgccttgt attcccaagg aaataggctc
     1081 agcgtattgc ccgacgaaat aggctcagcg ccaggctcgg accagttata tctttggatt
     1141 tattggataa gccaacgatc gaaggcctta tactttggtt tcgtcagctc gcgccatcgc
     1201 ctgagctttg agctcagatc ggctttagcg ggtttgcgat gccgcgtgca aaaccgctgt
     1261 ggcgcggcag ccacaaggat agaaattttc tgccaaggcc ggacggtgtc ttgtaactgt
     1321 ctttttctaa gtcaggatgg tcgatgttta ggcagggggg tctgccatca ccaagctcgg
     1381 catattcgct gcaacgatcc tcgcggccgc tggcgcctgc tgcggagtgt cggccgccca
     1441 cgccgcgccc aagacgtgca ctggcctttg ggactttatc gaaacggact gccagctgac
     1501 ctggtacggc atcacgctct acggcgtgat tgacgcgggg ttcacctacc agactcacgg
     1561 cgcgccgctt gatccgcgaa gccctccggg gtccgcatac atcgtccaga gatacagccg
     1621 gtcgtcgcgc tgggatctcg caccgaacgg gttgcaaaac tcgttcgttg gcatcaaggg
     1681 aactgagccg atcggcggaa acacctctgt agtctttgct ctggacgccg gcttcgatcc
     1741 ttattcattc aaattgtcca acggaccggg atcggctgct cacaacgccg gcgttcccca
     1801 ggaccagcaa acctcgtggg cggattcaag ccgagcgggg cagtggtaca acggcaacgg
     1861 ttatgtcggc ctcagctcgc cgagctatgg cacgctgacg gtattccggc agaacgtgct
     1921 gacctacgac ggtgtcctcg agtacgatcc gatgggcgcc tcttacgcct tctcgcccat
     1981 cggctggcag gggttaacct gcggcggcgg caataccgaa aactgcaggc acacgacgtc
     2041 gctgaaatac cggctcacgg tgggccaggc
//
LOCUS       DATLJT010000060         5608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3908340,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000060 DATLJT010000000
VERSION     DATLJT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908340"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VM910_01235"
     CDS             complement(<1..795)
                     /locus_tag="VM910_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HVI61207.1"
                     /translation="MTRIAVGGFLHETNTFAPTKATYQDFVHGGGWPAMAVGPDVFRA
                     MHRINTGLSGFIEAAKDEGWDLAPTVSCGASPSAHVTRDAFERIVKSMVDGIASAGPL
                     DAVYLDLHGAMVSEHFDDGEGEILARVRKIVGKDLPLVISLDLHANVTPQMVELADAL
                     IAYRTYPHVDMAMTGRAAARHLALLLKTKRRLAKAFRQVPFLIPISWQCTNDRPAKGI
                     YEKLAALQSEAVPTLSFAPGFPAADFEHCVPSVFAYGLTQKDADAAA"
     gene            complement(950..2593)
                     /locus_tag="VM910_01240"
     CDS             complement(950..2593)
                     /locus_tag="VM910_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61208.1"
                     /translation="MTDTVLDIRDLVVSLGRNAGATRIIDGVSLDVGRRETLCVVGES
                     GSGKSVTSLSVMGLLQKGSLVPTGGSIRLVGEELLTASDRRMRQLRATRMAMIFQEPM
                     TALNPVLPVGRQIDEVLRAHTDLDARTRRKRILDMMDQVRLPEIERIFASYPHKLSGG
                     QRQRIMIAMALVLEPKLLIADEPTTALDVTTQKQILTLIRDLQRDHGTAVLFITHDMG
                     VVAEIADRVAVMRGGRLVETGSLDSILRAPTNEYTRNLLVSVPSLVPRAPRPETKEPV
                     VLEANDLGKVYRERSFLGSTREVTAAKDVTLILRKGRTLGIVGESGSGKSTVARCIVR
                     LIDPTSGGIRLAGCEISELSRRLLQPHRKKIQIVFQDPYRSLNPRVTVGETIAEGPIN
                     YGTPRAEALEKARELLELVDLPADAISRYPHQFSGGQRQRIAIARAIALDPDVLVADE
                     AVSALDVSVQAQVLELLDEIQGRLGIALLFITHDLRVAAQICDDVAVMQHGRIVEQGP
                     AGEVLTNPKQAYTRALLDAAPGRGWDFANFRPVAATAAE"
     gene            complement(2590..3483)
                     /locus_tag="VM910_01245"
     CDS             complement(2590..3483)
                     /locus_tag="VM910_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61209.1"
                     /translation="MAIETLPGPSIPITRPFRSTLGFLAATPIIATATACLLLIVAMA
                     ILAPYLAPHDPLLLAPAQRLKPSSAEFLLGTDAYGRDLLSRIIFGARISLLIGVGAAI
                     VSVGIGLVIGLVSGFFKWVDAVMMRVMDGLMAIPAILLAIAVVSLSGASVVTVLVAIT
                     IPEIPRVARLVRAVVLSAREEPYVEAAISVGSGLPKIMWRHLMPNTVAPLIVQGTYVC
                     ASAILTEAILSFLGAGLSPETPTWGNIIAEGRSYFQIKPSLIFWPGLLLSITILSVNL
                     IGDAARDALDPRMKQREGGKA"
     gene            complement(3496..4437)
                     /locus_tag="VM910_01250"
     CDS             complement(3496..4437)
                     /locus_tag="VM910_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61210.1"
                     /translation="MLGYLLRRVLAAIPVMGVVALFVFLLLRLTPGDPAAILAGDNAT
                     PEQLERIRVSLGLNQPIYIQFFTWIDKLLHGDLGVSLISNVPVLKMIGQRIEPSISIA
                     LSTIILSILIAVPLGVVAAWKHGTWIDRFVMGLSVLGFSVPVFVIGYVLIQIFAIDLR
                     WLPVQGFRSITAGFGPFLERLVLPTLTLSFIYVALIARMTRAAMLDVLGEDYIRTARA
                     KGIGEIAVLLRHGLRNAAVPVITVIGSGFALLISGVVVTESVFNLPGIGRLTVDAVLA
                     RDYPVIQAMILLTSLLYVTVNLLIDVAYTLLDPRIRY"
     gene            complement(4604..5329)
                     /locus_tag="VM910_01255"
     CDS             complement(4604..5329)
                     /locus_tag="VM910_01255"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVI61211.1"
                     /translation="MAKAQTRAPKKSGAIPFKTILTRAEKRKGGSGGLQKLLPKKPSA
                     RALEKLSDDRFLSEMTRRVFCAGFAWSVIEAKWQGFEDAFLNFHPAKLTFQPDDYWDG
                     LLKDARIVRNGAKISSVRENAALVQAIAKEHGSFGKFLANWPSSDEIGLLDLLAKRGS
                     RLGGNTGQMFLRFVGWDGFVTSQDVVACLRDAGLDIASEVKSKGDLAKVQAQFNAWAE
                     ETGLPYVHLSRICAMSIGENRMP"
     gene            complement(5335..>5608)
                     /locus_tag="VM910_01260"
     CDS             complement(5335..>5608)
                     /locus_tag="VM910_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI61212.1"
                     /translation="GVTRPVWEPNEATMALYERAKRLAKQIGMELPHGSAGGGSDANF
                     TGAMGIPTLDGLGPRGADPHTLNEHIVVESLAERGRLMAGLLATLE"
BASE COUNT         1102 a         1722 c         1793 g          991 t
ORIGIN      
        1 cgccgcggcg tcggcatctt tctgcgtcag gccgtaggca aatacgctcg gcacgcaatg
       61 ctcgaaatcg gcggcaggaa atcccggcgc gaaagagagc gtcggcaccg cttcgctttg
      121 aagcgccgca agtttctcat aaatgccttt ggcgggccga tcattggtgc attgccaact
      181 gatcggaatc agaaagggca cttgccggaa tgccttcgcc aggcgccgct tggtcttgag
      241 cagcagcgcg agatgcctcg cggcggcgcg gcccgtcatg gccatgtcga catgcgggta
      301 agtgcgatag gcgatcagtg cgtcggcaag ctcaaccatc tgcggcgtga cattggcgtg
      361 caaatcgagg ctgataacca aaggcagatc cttgccgacg attttccgca cgcgtgccag
      421 aatttcacct tcgccgtcgt cgaaatgttc gctcaccatc gcgccatgga ggtcgagata
      481 gacggcatcg agcggaccgg cggatgcaat gccgtcgacc atcgacttca cgatacgctc
      541 gaaggcatcc ctggtgacgt gtgcagaggg gcttgcaccg caggaaacgg tcggcgccag
      601 atcccagccc tcgtctttgg ccgcttcgat gaaacccgac aatcccgtat tgatgcggtg
      661 catggccctg aacacgtccg gcccgacggc cattgccggc cagccgccac cgtgcacgaa
      721 gtcctggtag gtggccttcg tcggcgcaaa ggtgttggtc tcgtgcagga agccgccgac
      781 cgcgatgcgg gtcatcaacg ctgtctcagt gatgtgttcg tgcgcaggac gttagccctg
      841 catgagcgag aagcgcaagc gaggaagcaa ttccattttg catgacccgc aacgaggtct
      901 gtcattacgg gacgctgcct agcaacgcta cggaatgatg acgcggggtt tactccgcag
      961 ccgttgcggc aaccggcctg aaattcgcaa aatcccagcc gcgaccgggg gcggcgtcca
     1021 gcagtgcgcg ggtataggcc tgtttcggat tggtcagcac ctcgccagcc gggccctgtt
     1081 cgacgatgcg accatgttgc atcacggcaa cgtcgtcgca gatttgcgcc gcgacacgaa
     1141 gatcgtgggt gatgaacaac aacgcaatgc cgaggcgccc ctggatttca tcgagcagtt
     1201 ccagcacctg tgcctgcacg gaaacgtcga gcgcggagac ggcttcgtct gcgaccagca
     1261 catcgggatc gagcgcgatc gcacgcgcga tcgcgatgcg ctggcgctgg ccgccggaga
     1321 attgatgggg atagcgcgag atcgcgtccg ccggcagatc gacgagctcg agcagctcgc
     1381 gggccttttc cagcgcctcg gcgcgcggcg tgccatagtt gatgggacct tccgcgatgg
     1441 tctcgccgac ggtgacgcgc ggattgagcg aacgataggg atcctggaac acgatctgga
     1501 ttttcttgcg atgcggctgc aacaggcgac gcgacagttc cgagatttcg catcccgcaa
     1561 gacggattcc gcccgaggtc ggatcgatca gccgcacgat gcagcgcgcc accgtcgatt
     1621 tgccggagcc gctctcgccg acgatgccga gcgtgcggcc cttgcgcaag atgagcgtga
     1681 cgtccttggc agccgtcacc tcgcgtgtgc tcccgaggaa ggaacgctcg cgatagacct
     1741 tgccgaggtc gttagcttcc agcaccaccg gctctttcgt ttccgggcgc ggcgcgcgcg
     1801 gcaccaggct cggcacggag acaagcaggt tgcgggtata ttcgtttgtc ggcgcgcgca
     1861 ggatgctgtc gagcgagccg gtctcgacca gccgcccgcc acgcatgacc gcgacgcggt
     1921 cggcgatctc ggcgacaacg cccatgtcgt gggtgatgaa cagcacggca gtgccgtgat
     1981 cgcgctgcag gtcgcggatc agggtcagga tctgcttttg cgtggtgacg tcgagcgcgg
     2041 tggtcggctc gtccgcgatc aggagtttcg gttccagcac cagtgccatc gcgatcatga
     2101 tgcgctggcg ctggccgccg gagagcttgt gcggatagga agcgaagatg cgctcgatct
     2161 ccggcaggcg gacctgatcc atcatgtcga ggatacgttt gcgacgcgtg cgcgcatcga
     2221 gatcggtgtg cgcgcgcaac acctcgtcga tctggcggcc gaccggcaac accggattga
     2281 gggccgtcat cggctcctga aagatcatcg ccatccgcgt ggcgcgcaat tgccgcatgc
     2341 ggcgatcgct cgccgtgagc agctcttcgc cgacaagcct gatgctgccg ccggtcggca
     2401 ccagcgaacc tttttgcagc aggcccatca cgctgagcga ggtcaccgat ttgccggagc
     2461 cgctttcacc gaccacgcac agcgtctcgc gtcggccgac gtcgagcgac acgccatcga
     2521 tgatccgcgt cgcgcctgca ttcctgccga ggctgacgac gagatctctg atgtcgagta
     2581 cggtgtcggt catgccttgc cgccctcgcg ctgcttcatc cgcggatcga gcgcatcgcg
     2641 cgcggcatcg ccgatcaggt tcacgctgag gatcgtgatc gacagcaaaa gtccgggcca
     2701 gaagatcaag gacggcttga tctgaaaata ggaacgccct tcggctatga tgttgcccca
     2761 ggtcggggtt tccgggctga ggccagcgcc gaggaacgac aggatcgctt cggtgaggat
     2821 cgccgacgcg cagacataag tgccctggac gatcagcggc gcgaccgtgt tcggcatcag
     2881 atgccgccac atgatcttgg gcagcccgga gccgaccgag atcgcagcct cgacataggg
     2941 ctcctcccgc gccgagagga cgactgcacg caccagccgc gccacgcgcg gaatttccgg
     3001 aatggtgatg gcgaccagga cggtcacgac gctggcgccc gacagcgaga cgactgcgat
     3061 ggcgagcaga atcgccggga tcgccatcaa tccatccatc acgcgcatca taacggcatc
     3121 gacccatttg aagaagccgg agacgagccc gatcacgagt ccgatgccga ccgagacgat
     3181 tgcggcccca accccgatca acagcgaaat ccgtgcgcca aagatgatgc gcgacaacag
     3241 atcgcggccg taggcgtcgg tgccaagcaa gaactccgcg ctcgacggtt tcaggcgctg
     3301 cgctggcgcg agcaacagcg gatcgtgtgg ggcgaggtag ggcgcgagaa tcgccatcgc
     3361 gacgatcagg agcaggcagg cggtggcagt tgcaatgatc ggcgttgccg cgaggaagcc
     3421 gagggtcgac cggaacggcc tggtgatcgg tattgacggg cctggtaagg tctcgattgc
     3481 catcggtacc tgacctcagt agcggattct gggatcaaga agcgtgtagg cgacgtcgat
     3541 caagagattg acggtgacgt agagcagcga ggtcagaagg atcatcgcct ggatcaccgg
     3601 atagtcgcgc gccaacacgg catcgacggt gaggcggccg atgccaggca ggttgaacac
     3661 gctttcggtc acgacgacgc ccgagatcag cagcgcgaag cctgagccga tcactgtgat
     3721 gacggggacc gcggcattgc gcaagccatg gcgcagcagg actgcgatct cgccgatacc
     3781 cttggcgcgc gcagtacgga tgtaatcctc gccgagcacg tcgagcatgg ccgcccgtgt
     3841 catgcgcgca atcagcgcaa catagatgaa ggagagggtg agcgtcggca gcacgagacg
     3901 ttcgaggaac ggcccaaagc ccgctgtgat actgcggaag ccctgtaccg gcagccagcg
     3961 caggtcgatc gcaaagatct ggatgagaac atagccgatc acgaataccg gcacggagaa
     4021 gccgagcacc gacaatccca tcacgaaacg gtcgatccac gtgccgtgtt tccaggctgc
     4081 gaccacgccg agcggcacgg cgatcaggat ggagaggatg atggtcgaga gcgcgatcga
     4141 gatcgagggc tcgatacgct ggccgatcat cttcaaaacc ggaacattgg agatcaggga
     4201 aacgccgagg tcaccgtgca gcagcttgtc gatccaggtg aagaactgga tgtagatcgg
     4261 ttgattcagg ccgagcgaaa cacggatgcg ctcaagctgt tccggagttg cattgtcgcc
     4321 ggcgagaatt gcggcgggat cgcccggcgt cagccgcagg agtagaaaca cgaacaatgc
     4381 gaccacgccc atgacgggaa tggcggcaag cacgcggcga agcaaataac cgagcatcga
     4441 aacgccaatc tctcggcagc ggtcgtcaaa tcatgcggac ttcggaatct aactgaaggc
     4501 ttttgtaggt ccttggcaat ccgccatttt acgataggcc tgttttttgg ctgccgctcg
     4561 cgctgcaagc cccatgccat gaccagggca gggagccgtg gcttcagggc atgcggttct
     4621 cgccgatcga catggcgcag atgcgcgaca agtgcacgta gggcagcccg gtttcctcgg
     4681 cccaggcgtt gaactgcgcc tgcaccttgg caaggtcgcc cttggatttg acttcgcttg
     4741 cgatgtcgag gcctgcatcg cgcaggcagg ccaccacatc ctgcgacgtc acgaatccgt
     4801 cccagccgac aaagcgcaaa aacatctgac cggtgttgcc gccgagccgg ctgcctcgct
     4861 tggcgagaag gtcgagcagg ccgatctcgt cggacgaggg ccagttcgcc aaaaatttcc
     4921 cgaagcttcc atgctctttc gcgatcgcct ggaccaaagc cgcattctcg cgcacggatg
     4981 agatttttgc gccgttgcgc acgatgcgcg cgtccttcaa caggccgtcc cagtagtcgt
     5041 cgggctggaa ggtcagcttg gccggatgga agttgagaaa agcatcctca aagccctgcc
     5101 acttcgcttc gatcacgctc caggcaaatc ctgcgcaaaa cacccgcctg gtcatctcgg
     5161 agagaaagcg atcgtccgaa agcttctcca gcgctctggc gctgggtttc ttcggcagca
     5221 gcttttgcaa accgccggag ccgcctttgc gcttctcggc acgcgtcaaa atggtcttga
     5281 acgggattgc gccggatttt tttggcgccc tggtctgtgc cttcgccatc agtcctattc
     5341 cagcgttgcc aatagtccgg ccatcagccg gccgcgttcg gcgaggcttt ccaccacgat
     5401 atgttcgttg agcgtgtgcg ggtcggcacc gcgcggaccc aggccgtcga gtgtcgggat
     5461 gcccattgca ccggtgaaat tcgcgtccga gccgccgccc gcgctgccgt gcggcagttc
     5521 catcccgatc tgctttgcga ggcgcttcgc cctctcgtaa agcgccatcg tcgcttcgtt
     5581 cggctcccat accggccgcg tcacgccg
//
LOCUS       DATLJT010000061         3241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2300194,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000061 DATLJT010000000
VERSION     DATLJT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300194"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="cbiB"
                     /locus_tag="VM910_01265"
     CDS             complement(<1..657)
                     /gene="cbiB"
                     /locus_tag="VM910_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HVI61213.1"
                     /translation="MAVALTLLALLIEAMAGYPDRLVRSIGHPVIWMGSLIAALERRW
                     NRETAAPALRRTLGAVAIVLLVGIVGVAAFIIERGLLILPFGLLGAGILGSTLIAQRS
                     LRQHVARVADALDNAGLASGREAVSHIVSRDTNALDEAGVARAAIESLAENFSDGVVA
                     PVLWMIIAGLPGAAIYKAVNTADSMIGHRTARFQAFGWAAARFDDLLNLPASRLSALL
                     I"
     gene            641..1696
                     /gene="cobD"
                     /locus_tag="VM910_01270"
     CDS             641..1696
                     /gene="cobD"
                     /locus_tag="VM910_01270"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HVI61214.1"
                     /translation="MSATAMISRSEATPLEHGGDLAAARRLFPDAPQPFIDLSTGINP
                     YPYPVPPLAANVFTRLPDSAAQVRLREIAARTYGAPSAAHMVCAPGTQILLPLLAALV
                     PPGRAGVLGPTYSEHARAAALAGHAVMDVRDINALRAVDLAVVVNPNNPDGRIVTKER
                     LLALSHDLSARGGILAVDEAFMDVGPPGASLAGEIHRGNIIVLRSFGKFFGLPGLRLG
                     FALAAPDLAAKLNATLGPWAVSGPAIAIAERALADHAWIDATRIRLASAAGKLDRLLA
                     EANVEVLGGTALFRLVRSRRAHEIFQHLGAAGIIVRAFREQPTWLRFGIPSESSWDRV
                     RSRLATYGKLAGTATTP"
     gene            1826..2473
                     /locus_tag="VM910_01275"
     CDS             1826..2473
                     /locus_tag="VM910_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVI61215.1"
                     /translation="MGTIITVATMKGGSGKSTLASCLAVHWHLDGRHPTIIDADPQRS
                     IARLAARERRLGGVPVVEDATEHACEAARRLAVAGGPVIIDTPGFRLETTLTCLAASD
                     FVLVPVKPSPFDVDRMLDTLNMLINGASDWRPIFRCLLTQTTRESVIAKHIRSELATA
                     GFPLLESELTNRVVYAEAALWGATPSLVDRTGPAARDIAAIAAEVDNILAIKRAA"
     gene            2674..3150
                     /locus_tag="VM910_01280"
     CDS             2674..3150
                     /locus_tag="VM910_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HVI61216.1"
                     /translation="MPVTAPPTHGARKFAETNLAKRQARARAIVARHAAYSAVGGIVP
                     LPVASVAGITTIIIRMVKMLSDLYGVPFERDRARAIIVGLVGGTVPTGFAAVTTSTLF
                     FAVPSGALMGLVVSSVTAIACTRSIGQIFIEHFESGATLHESSVKNSVSGQFELCG"
BASE COUNT          606 a         1027 c         1010 g          598 t
ORIGIN      
        1 gatgagcaga gccgagagcc gcgatgccgg caggttgagc aagtcatcga agcgtgcagc
       61 cgcccaaccg aaggcctgga aacgcgcggt acgatgcccg atcatgctgt ccgccgtgtt
      121 gacggcctta taaattgccg caccggggag cccagcgatg atcatccaga ggacaggcgc
      181 aaccaccccg tcggaaaaat tctccgccag gctttcgatt gccgcacggg caacacctgc
      241 ttcgtcgagc gcgttggtgt cgcgactgac gatgtgcgat accgcttcgc gcccgctggc
      301 gaggccagca ttgtcgagcg cgtcggcaac acgcgccacg tgttggcgca gacttcgctg
      361 ggcgatcaac gtgctgccga gaatcccggc accgagcagg ccgaacggaa gaatcaggag
      421 tccgcgctcg atgatgaacg ccgccacgcc gacgatgcca acgagcagaa caatggcgac
      481 agctccgagc gtgcgtcgca gcgcgggggc cgccgtttcg cggttccatc ggcgctccag
      541 tgccgcgatc aagctgccca tccagatcac gggatgacca attgatcgca ccaggcgatc
      601 cggataaccg gccatagcct cgatcagcag ggcgagaagc gtgagcgcga cagccatgat
      661 ctcccgttcg gaagccacgc cgctcgaaca tggtggcgac ctggcggcag cgcggcggtt
      721 gtttcccgat gcgccgcaac ctttcatcga cctctcaacg ggaatcaatc cctatcctta
      781 tcccgtaccg ccccttgccg ccaacgtgtt cacgcgactg cccgattccg cggcgcaggt
      841 tcggctccgc gagatcgcgg cacgaaccta cggggctcca tcggccgcgc atatggtgtg
      901 tgcgccgggc acccagatcc tgttgccatt gctggcagcg ctcgtgcccc ccggtcgcgc
      961 gggcgttctc gggccgacct attccgagca tgcgcgggcc gcggccctcg ccgggcatgc
     1021 cgtcatggat gtccgcgaca tcaatgcgct gcgcgcggtt gacctcgccg ttgtcgtcaa
     1081 tccgaacaat ccggatggcc gcatcgtcac gaaagagagg ctgctggctt tgtcgcacga
     1141 tttgagcgcc cggggtggca ttctcgccgt cgacgaagcg ttcatggatg tcgggccgcc
     1201 aggggccagc ttggcgggcg aaatacaccg cggcaacatc atcgtgctgc ggtcctttgg
     1261 gaaattcttc ggtctcccag gcctgaggct gggctttgcg ctcgccgcgc cagatctcgc
     1321 agcaaagctc aatgcgacct tgggcccgtg ggcggtatcc ggtccggcta tcgccatcgc
     1381 cgaacgggcc cttgcagatc atgcttggat cgacgccact cgcatccgac tggcgagcgc
     1441 cgcgggaaag ctcgaccgcc tgttggcgga ggcaaacgtc gaggttctcg gcggcacagc
     1501 gttgttccgg ctggttcgct cgcgccgcgc ccatgagata tttcagcatc ttggagccgc
     1561 cggaatcatt gtgcgcgcct ttcgcgaaca accgacatgg ctgcggtttg gtattccgag
     1621 cgaatccagc tgggatcgtg tgcgttcgcg actcgcaacc tacggcaagc ttgccgggac
     1681 ggcgaccacg ccgtgaaagg cgcattgaag gggggcccgg tcctctttct cagcctaata
     1741 ttggcaacag taggcagttc ttcaagaaaa cgataacatc gattcggatg attcgttgcg
     1801 ttggcggccg ggaaaccgac aagagatggg cacgatcatc acagtggcaa ccatgaaggg
     1861 cggcagcggc aaaagcacgc tcgcaagctg ccttgcggtt cattggcatc ttgatggacg
     1921 ccacccgacg atcatcgatg ccgatccgca gcgctcgatc gcacgcctcg ccgcgcgcga
     1981 acggcggctc ggcggggtgc ccgttgtcga ggacgcgact gagcacgcct gcgaagcggc
     2041 acgccgtctc gccgtcgccg gcggtcccgt catcattgat acaccgggct ttcgcttgga
     2101 gacgacgctg acatgtctcg ctgcaagcga ttttgtcctg gtcccggtta agccctcacc
     2161 gtttgatgtc gatcgcatgc tcgatacact caatatgctc attaacggtg cgagcgactg
     2221 gcggccgatc ttccgctgtc tgcttaccca aacgacgcgt gagtccgtca ttgccaagca
     2281 cattcgttcc gagctcgcca cggccggctt cccgctgctg gaaagcgagc tgacgaaccg
     2341 tgttgtctat gcggaagccg cactctgggg tgcgacgccg agtctcgtcg accgcactgg
     2401 acccgctgcg cgcgacatcg cggcgatcgc agcggaggtc gacaacatcc tcgccatcaa
     2461 acgggcggca tgagcgtgag taagaaaaaa cttcctaacg caatcatgca cccgatcgat
     2521 gggatgcgtg aggccgtggc cgctgatcgc tcacgggagt ggcgaggccc gctcacctcc
     2581 acgacggcgt cgaatcaatc ggcaaatgga ggggcaatca cggcgcggtc cggtcctgtc
     2641 ggcgaggtga ttgaggagac gcgagtctct acaatgcccg tcacggcgcc tccaacccac
     2701 ggcgcgcgaa agttcgctga gaccaatttg gcgaaacgcc aagcacgagc gcgtgcgatc
     2761 gtagcgcgcc atgccgccta ctcagcggtt ggcggaattg tcccgcttcc agtagccagc
     2821 gttgctggca ttacgaccat catcatccgc atggtgaaga tgctgagcga tctttatggc
     2881 gtgccgttcg agcgtgatcg agctcgcgcc atcatcgtag gcctcgttgg aggcaccgtg
     2941 cccactggat ttgccgcagt cacgacatca acgctttttt tcgccgtgcc gtccggcgcc
     3001 ctcatgggac ttgtggtctc ctcggtcacg gcgattgctt gcacccgcag cattggccag
     3061 attttcatcg agcatttcga aagcggggcg acgttgcatg agtcgtccgt gaagaactcg
     3121 gtttcaggcc aatttgagct gtgcggctag gccgagagcg atcagaattc tgagcaacaa
     3181 aaacctcaac cacgcgagga ggcggcggaa attatatccg gcggcggcga gcacggcgtt
     3241 g
//
LOCUS       DATLJT010000062         4921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381119,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000062 DATLJT010000000
VERSION     DATLJT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381119"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VM910_01285"
     CDS             <1..307
                     /locus_tag="VM910_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HVI61217.1"
                     /translation="TISKLKERRPELVIGFAAETEHLIDNAKAKLKRKGCDWIVANDV
                     SAASGVMGGDRNTVHLLTRNGADIAVDSWPVMTKEEVATALIAEVANRFGKSAGTSP"
     gene            304..762
                     /gene="dut"
                     /locus_tag="VM910_01290"
     CDS             304..762
                     /gene="dut"
                     /locus_tag="VM910_01290"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HVI61218.1"
                     /translation="MSAQVTIEIHQLPHGEGLPLPAYQSAHAAGLDLLAAVPTDAPLI
                     LSPGQYALVPTGLAIALPEGFEAQVRPRSGLAAKHGVTVLNAPGTVDADYRGEVSVIL
                     INHGSAPFTIARGERIAQMVIAQVANAELVPVVSLAPTGRGGGGFGSTGR"
     gene            976..3450
                     /locus_tag="VM910_01295"
     CDS             976..3450
                     /locus_tag="VM910_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HVI61219.1"
                     /translation="MSDVIVAMRRTLLSCTALVLPSSRAAAAGDLRLPHAVTTFFDLN
                     HQEVAVLTTAMALLGFSVVAAILLMRNRVRAARAEAKLRADIAELQVQADSYRALLFA
                     EPQVLISWAAGGNRPKISGDASLLLQHDTPQRILAFGTWLPPEPALQMDHAVDALRET
                     GESFLFHLTTTAGHAMEAMGRAIGGQAIVRIRELSGLRRELAETNHRLKALMEETEML
                     RGFAATAPWPIWAKGAKGSLTYANAAYAQATEAATVHDAIDRNLELLGTADRADMERT
                     LGATAAFAARLPIVARGERRIYDVRATNVGGGSVGIALDASEADALSSALVRMAEAHR
                     RTLDQLSSGVAVFDGQRRLAFYNDSYRRLWDLEPAFLDSNPDDATVLDRLRAARKLPE
                     QADFRAWKAKLHEAYRAMEPAKDTWYLPDGRALSVVTTPNPEGGVTYLFDDVTESLDL
                     ARRFDRLIRVQRETLDSLAEGVAVFGSNGKAQLFNPAFAKMWKLSPDALREEPHVDTV
                     ASWCRPLFDDAKGWRAIHEAITGIENRNDLSLKLERKDGSVLDCMSRPLPDGATMLTF
                     QDITDTENVERALRESNEALRAADQMKIDFVHHVSYELRAPLTTIIGFAHFLSDPSTG
                     PLTPKQAEYLDYVTKSTNALLALTNNILDLATIDAGAMKLELGPVDIGKAIAAATEGI
                     QDRLATDRIRLKVEIAPNIGSFVGDERRVVQVLYNLLANAVGFSPQGSTVGVSARRTE
                     RNVVFTVTDSGPGIPPDVKDKVFDWFESHSQGSRHRGAGLGLSLVRSFVELHGGKVRV
                     DSTVGKGTTVTCDFPIDQAAHRNAAE"
     gene            3447..>4921
                     /gene="tsaE"
                     /locus_tag="VM910_01300"
     CDS             3447..>4921
                     /gene="tsaE"
                     /locus_tag="VM910_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVI61220.1"
                     /translation="MTSLATFSVALSNEAATAHLMADLALLIGPGDVITLTGDLGAGK
                     TAAARALIRYLADDDGIEVPSPTFTLAQTYELPSFPVLHADLYRVSDASELDEIGLSP
                     LPEATVALIEWPERAASALPADRIDIALSHRPALGPTARAAEITGSGKGASVVERLRT
                     LRQFLTEVGFVEARRRRMAGDASIRSYARLIRDDGVFILMNFPKRPDGPAIYDGKSYS
                     AAVHLAEDVKPFVAIANGLRGRGFSAPMIHHADIDAGFLVTEDFGNAGFVDGDPPGPI
                     TERYEAATDMLAALHRQSLPDRLPLVPHVDYAIPVFDIEAWLVEIGLMLEWYLPDRGA
                     RPNDTLRTEFVAMWRELLQKPAAAAKTWVLRDFHSPNLIWLGERDDVARVGVIDFQDA
                     VLGPAAYDLVSLLQDARVDVPEQFELMLFTRYVKARRAADAKFDPADFAALYAIMSAQ
                     RNTRLLGTFARLNRRDGKPQYLKHLPRIWTYLSRSLAHPEL"
BASE COUNT          845 a         1664 c         1557 g          855 t
ORIGIN      
        1 cacgatctcg aaactgaagg agcggcggcc cgagctcgtc atcggctttg ccgccgagac
       61 agagcatctg atcgacaacg ccaaggccaa gctgaagcgc aagggctgcg actggatcgt
      121 ggccaacgat gtgtcggccg cgtcaggcgt gatgggcggc gaccgcaaca ccgtgcatct
      181 cctgacccgg aacggggccg acattgcggt agattcatgg ccggtcatga ccaaggaaga
      241 ggtcgcgacc gcgctgatcg ccgaagtcgc aaaccgcttt ggcaagagcg cggggacatc
      301 accttgagcg cacaggtcac gatcgagatc caccaattgc cgcatggcga ggggctgccg
      361 ctgccggcct atcagagcgc ccatgcggcg ggtctcgacc tgctcgcagc ggtcccgacg
      421 gatgctccgc tgatcctttc gcccggccag tacgcgctgg tgccgaccgg gcttgccatc
      481 gcgcttccgg aagggttcga ggcgcaggtg cggccgcgct cgggactggc ggcaaagcat
      541 ggcgtcacgg tgttgaacgc gccgggcacg gtggatgcgg attatcgcgg cgaggtctcc
      601 gtgatcctga tcaatcacgg cagcgcgccc ttcacgatag ctcgcggcga gcgcatcgcg
      661 cagatggtga tcgcgcaggt cgcgaatgcc gagctcgttc ccgtggtctc gctggcgccg
      721 acgggtcgcg gcggcggcgg tttcggctcg acgggacggt aattcgccgc aacacttcgc
      781 gcaaaaattc gccggatacg tcttagtcct gcttttttcc ccagcgagcc tcgcgttcac
      841 ggtctggact cttacccccc gagtcccctt tggcgatatt gttctttgat tcgcgcccgg
      901 tggggagttt gccgggcgca atgacgtacg gacatttttc gtgcgggctt ttttcgtgcg
      961 gtcttggggc aaaccatgtc ggacgtgatc gtggcgatgc gtcggacact gctgtcgtgc
     1021 acagcgctgg tgctgccgtc cagccgcgcg gctgcagccg gcgacctgcg cctgccgcat
     1081 gcggtcacga ccttcttcga cctcaaccac caggaagttg cggtcttgac cacggccatg
     1141 gcgttgctcg gcttctccgt ggtggcggcg atcctcctga tgcgaaaccg cgtccgcgcc
     1201 gcccgcgcag aggcgaagct gcgcgccgac atcgccgaac tgcaggtgca ggccgattcc
     1261 taccgcgccc tgctgttcgc ggaaccgcag gtcctgatct cctgggcagc cggcggcaat
     1321 cgcccaaaga tctcgggcga cgcctcgctt ctgctgcagc acgacacacc gcaacggatt
     1381 ctcgccttcg gaacctggct gccgccggag ccggcgctgc aaatggacca tgccgttgac
     1441 gcgctccgcg agaccggcga aagttttctg ttccacctga ccaccaccgc cggccacgcc
     1501 atggaggcga tgggtcgcgc gatcggcggc caggccattg tccggattcg cgagctttcg
     1561 ggcctgcggc gggagctggc ggagacgaat catcggttga aggcgctgat ggaagagacc
     1621 gagatgctgc gcggctttgc agcgaccgcg ccctggccga tctgggcgaa gggcgccaag
     1681 ggctcgctga cctatgccaa tgccgcctac gcccaggcga ccgaagccgc gaccgtccac
     1741 gacgcgatcg accgcaatct cgaactgctc ggcaccgccg accgcgccga catggagcgc
     1801 acgctcggcg cgaccgccgc cttcgccgca cggcttccga tcgtggcgcg cggcgagcgg
     1861 cggatctacg acgtacgcgc gaccaatgtc ggcggcggca gtgtcggcat tgcgctcgat
     1921 gccagcgagg ccgacgcatt gagctcggcg ctggtgcgga tggcggaggc acatcgccgc
     1981 acgctcgatc aattgtcctc gggcgttgcc gtgttcgacg gacagcggcg gcttgccttc
     2041 tataacgatt cgtatcgcag gctgtgggac ctcgagcccg ccttcctcga ctccaaccca
     2101 gacgacgcaa cggtgctcga ccggctgcgc gccgcacgca agctgcccga gcaagccgat
     2161 ttccgcgcct ggaaggcgaa gctgcacgag gcctatcgcg cgatggagcc tgccaaggac
     2221 acctggtatc tgcctgacgg gcgcgcactc agcgtcgtca ccacgcccaa cccggaaggc
     2281 ggcgtcacct atctgttcga cgacgtcacc gagagtctcg acctggcacg gcggttcgac
     2341 cgcctgatcc gcgtccagcg cgaaacgctc gacagccttg ccgaaggcgt cgcggtgttc
     2401 ggcagcaacg gcaaggcgca attgttcaac ccggccttcg ccaagatgtg gaagctgtcg
     2461 ccggacgcgc tgcgcgagga gccgcatgtc gacacggtgg cgtcctggtg caggccgctg
     2521 ttcgacgatg cgaaaggctg gcgcgcgatc cacgaggcga tcaccggcat cgagaaccgc
     2581 aacgacctct cgctgaagct cgagcgcaag gacggcagcg tgctcgactg catgagccgg
     2641 ccgctgcccg acggcgccac catgctgacc ttccaggaca tcaccgacac cgagaatgtc
     2701 gagcgggcgc tgcgcgaaag caacgaggcg ctgagggccg ccgatcagat gaagatcgat
     2761 ttcgtgcacc atgtgtccta tgagctgcgg gcgccgctca ccaccatcat cggcttcgcc
     2821 catttccttt ccgatccctc gactggcccg ctgacgccga agcaggccga atatctcgac
     2881 tacgtcacca aatcgaccaa cgcgctgctc gcgctcacca acaacatcct cgacctcgct
     2941 actatcgacg ccggcgcgat gaagctcgaa ctcggcccgg tcgatatcgg caaggcgatc
     3001 gcggcagcca ccgaaggaat tcaggaccgt ctcgccaccg atcgtatccg cctgaaggtc
     3061 gaaatcgctc ccaacatcgg cagcttcgtg ggcgacgagc ggcgcgtggt acaggtgctc
     3121 tacaatctgc tcgccaatgc ggtcggcttc tcgccgcagg gctcgaccgt tggcgtcagt
     3181 gcccgtcgca ccgagcgcaa cgtcgtcttc accgtgacgg actccggacc cggcattccg
     3241 cctgacgtca aggacaaggt gttcgactgg ttcgagagcc attcgcaggg gtcgcggcat
     3301 cgcggcgccg gcctcggcct gtccctggtg cgctcctttg tcgaactgca cggcggcaag
     3361 gtgcgcgtcg attcgaccgt cggtaaaggt acgacagtca cctgcgactt cccgatcgac
     3421 caggccgcgc accgcaacgc cgcggaatga cgtcgcttgc aacattctcg gtggcgctgt
     3481 cgaacgaggc ggcgaccgcg catctgatgg ccgaccttgc acttctgatc ggcccgggcg
     3541 acgtcatcac actgaccggc gatctcggcg ccggcaagac ggcggcggcg cgggcattga
     3601 tccgctatct cgccgacgac gatggcatcg aagttccgag tcccactttt acgctcgcgc
     3661 aaacctacga actcccgtcg tttcccgtgc tccacgccga tctctaccgc gtcagcgatg
     3721 cgtccgaact ggacgagatc ggactatcgc cgctcccgga agccacggta gcgctcatcg
     3781 aatggcccga gcgcgcagcc agcgcgcttc ccgccgatcg gatcgatatt gccttgagcc
     3841 atcgcccggc gctcggtcca accgcgcgtg cagccgagat cacgggctcc ggcaaaggtg
     3901 caagcgtcgt cgagaggctg agaaccttgc gacaattcct gacggaggtc ggctttgtcg
     3961 aagcgaggcg ccgacgcatg gcgggcgatg cttcgatccg ttcctatgcc cgcctgatcc
     4021 gggacgacgg cgttttcatc ctgatgaact tcccgaagcg gccggacgga cctgcgatct
     4081 atgacggcaa atcttacagc gccgcagtcc atctcgccga ggacgtgaag ccctttgtcg
     4141 ccatcgccaa tggcttgcgc gggcgcggct tctccgcacc gatgatccat cacgcggata
     4201 tcgacgccgg attcctggtg accgaggatt ttggcaatgc cggtttcgtc gacggcgatc
     4261 cacccggccc catcaccgag cggtatgagg cggcaaccga catgctggcc gcgctgcacc
     4321 gtcaaagtct tccggaccgg ctgccgctgg tgccgcatgt cgactatgcg attcccgtct
     4381 tcgacatcga agcctggctg gtcgagatcg gcttgatgct cgaatggtat ctgccggacc
     4441 gcggcgctcg gccgaacgac acgctgcgca ccgaatttgt cgccatgtgg cgcgaactat
     4501 tgcagaagcc tgccgcagcg gccaaaacct gggtgctccg cgattttcat tcgccgaacc
     4561 tgatctggct cggcgaacgc gacgacgttg cgcgcgtcgg agtcatcgat tttcaggacg
     4621 ccgtgctcgg gccggcggcc tatgacttgg tatcgctgtt gcaagacgcc cgcgtggacg
     4681 tgccggagca gttcgaactc atgctgttca cccgctacgt caaggcacgc cgcgcggccg
     4741 acgcaaagtt cgaccctgcc gatttcgccg cgctctatgc catcatgtcg gcacagcgca
     4801 acacgcggct gctcggcacc ttcgcgcggc tgaaccgccg cgacggcaag ccgcaatatc
     4861 tcaagcacct gccccggatc tggacctatc tcagtcgttc gctggcgcat ccggagctgg
     4921 a
//
LOCUS       DATLJT010000063         4783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381123,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000063 DATLJT010000000
VERSION     DATLJT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381123"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..274
                     /locus_tag="VM910_01305"
     CDS             17..274
                     /locus_tag="VM910_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61221.1"
                     /translation="MKPTTTIVLLTLLALPANAQDGGRIFHDAAGRVTGSARTDANGV
                     TTFHDRAGFVTGTARTDSNDTTTFHDASGRVTGRSGVKANG"
     gene            657..956
                     /locus_tag="VM910_01310"
     CDS             657..956
                     /locus_tag="VM910_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HVI61222.1"
                     /translation="MVSNEDRAIVEALNEPRRRCTSVFTTGGTGPTHGDITADCVAKA
                     FGVPIDTDTRALAILHEWVKTTGAKMNEARLRMTRIPKGVDLILNKVSGAWLLDR"
     gene            complement(1048..1548)
                     /locus_tag="VM910_01315"
     CDS             complement(1048..1548)
                     /locus_tag="VM910_01315"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVI61223.1"
                     /translation="MTKQQKWTPESPIEACATTYESLAKALGGLQNKFEIPEAAREFV
                     KRSAAAAKDRSSDLHAGANKATGAVEEALVGAVNGLADLNRKIVDATHQDLEAAFAAI
                     DKLASAESLTDAFETYVDYLRQQSKVGVARAKDAASLVSTKASEGFDALRDGIANFMT
                     VRSQAA"
     gene            1807..3117
                     /locus_tag="VM910_01320"
     CDS             1807..3117
                     /locus_tag="VM910_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HVI61224.1"
                     /translation="MGDADSSQGRKTLTAAALDPHDRARLRELKRTKWLAGGVLVASL
                     TVLVIAKLLERHNAGFGFLAAFTEAATIGGLADWYAVVVLFRRPLGLPIPHTAIIPAN
                     HREIAERLGGFIETHFLAPEPVGAKLRRVDFAVAASEWLRDPERSDGLARLILRLLPQ
                     ALTAAEHSGLRGFLARQLVDQIEAVKLAPLTAELMTTFTQDQRHQRLLDEVLLALNRL
                     MTDPVTLNAIRQKIRAELPTLLNLYRADRFLLKKIATSAFTFLEEVRADKNHPLRREF
                     ERFVASFIDKIASSPEYAARLETFKRDLIADPRVGDLAEGMWTSFRRLLEQNVRGSNS
                     VLHAHLRGLLIEAGRKLADDPRLRAHINRGMVAVLENFVEDHKGGAATFIAEQIKAWD
                     MDHLVRLIEINVGKDLQFIRFNGAIVGGLAGLAIYTAEILAKLA"
     gene            3399..>4783
                     /locus_tag="VM910_01325"
     CDS             3399..>4783
                     /locus_tag="VM910_01325"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HVI61225.1"
                     /translation="MAGKLIGVARAAYQYPLLIKQLLHAPMVQNPDQQIVYRDLRRLK
                     YSELHDRIGRLANLLKMLGVRPGDTVGVMDWDSNRFLEAFFAVPMMGAILQTVNIRLS
                     PEQITYTINHAGASVLLVNDEFVGLVQGIKSQLDKVRTFVVLTDRPEPAKGDIGFAGE
                     YEELLAAASADYDFPDFDENTQATTFYTTGTTGLPKGVYFSHRQLVLHTLAELAAFGS
                     APEQGRFHRNDVYMPITPMFHVHAWGFPWAATAAGVKQVYPGRYAPDVLLKLIKTEGV
                     TFTHGVPTILQMLLAAAEKADVDLSGLTMVIGGSSLSKALALKALDRGIDIYAGYGMS
                     ETGPVLTVAHLKSHHLTGNPDAQVEIRTRAGLAVPLVDLRIVDPDMKEMARDGKATGE
                     IVARAPWLTQGYFNNLEASEQLWAGGYLHTNDIGNIGPDGYVQITDRIKDVIKTGGEW
                     VSSLELEDIIS"
BASE COUNT          942 a         1500 c         1461 g          880 t
ORIGIN      
        1 ggggtaatca acgaccatga aacccaccac caccatcgtg ctcttgaccc tcttggcgct
       61 tcccgccaac gcgcaggacg gcgggcgcat ctttcacgac gccgccggtc gcgtcaccgg
      121 aagcgccaga accgacgcca acggcgtcac gacattccac gaccgcgcgg gcttcgtcac
      181 cggtaccgca cgcaccgata gcaacgacac gaccacgttc cacgacgcca gcggtcgcgt
      241 cacagggcga tcaggagtga aggccaatgg ctaagcacga accctcgatc ggcgccacgt
      301 cggagtggta ggcccccaac ctcgccctct cacggcgaaa acaggggttt cgagtcccta
      361 gggagcgcca atgatttcaa cgatttagtg gtattaaagc ctgttaggtt atcggcatct
      421 ccaacttttc tccaatgagc ggtgcctccg aagatcggtt cgaggccacc cggcgatcgt
      481 cggctcgagg aggccgaaga gatggcgcag tcgccgccga gccggcatcg gtgcttactt
      541 tctaaagatg acccacgccc gcatcgcggc tcgcgctccg cggagcaaat tgaacgcgcg
      601 ccctgtctgg gcgtgagaag acgacggcgg tcggcatcga tctcaaggaa gtgcgggtgg
      661 tcagcaatga ggacagagcg atcgtcgagg cactcaacga gccccggcgc cgctgcacct
      721 ctgtcttcac caccggcggg accgggccga cccacggtga catcaccgcc gactgcgtgg
      781 cgaaggcgtt cggtgtgccg atcgacactg atacgcgcgc gctcgccatc ctgcacgaat
      841 gggtgaagac gaccggcgcc aagatgaacg aggcacgcct ccgcatgaca cgtattccca
      901 agggcgtgga tctcattctc aacaaggtgt cgggggcgtg gcttctggat cggtaatgta
      961 atcgtgctgg cgggcgcgcg gcccaaacaa attggagggc ccgctcacgc gggccctcgg
     1021 caaattgtac gcccgaaggg cggcggatca ggcggcctga gagcgcaccg tcatgaagtt
     1081 agcgataccg tcacgtagtg cgtcaaaccc ttccgacgcc ttcgtgctca ccaagctcgc
     1141 cgcatccttc gcacgagcga caccgacctt gctctgctga cgcagatagt cgacgtacgt
     1201 ctcaaaagcg tcggtgagtg actcggcgct ggccagcttg tcgatcgccg cgaacgccgc
     1261 ctcgaggtct tgatgcgtcg cgtcaacgat cttgcgattg agatcggcca ggccgttcac
     1321 cgccccgacg agggcctcct cgaccgcgcc ggtggccttg ttggcgcccg cgtgcaggtc
     1381 ggaactgcga tccttcgcgg cagcggcgct gcgcttgacg aactcgcgtg cggcttccgg
     1441 gatttcaaac ttgttctgaa gcccgccaag cgccttcgca agcgactcgt aggtcgtggc
     1501 gcaggcctcg atcggggact caggcgtcca tttctgctgc ttcgtcatct gacttctcca
     1561 tcctcattga gagctaatgg tgaccccgtc cggtatcggc ccgaggaata ccccaccctt
     1621 gcacgattta tgttgcatcg caacataaat tgtgcattgc acaaaattgt gatcgcgacg
     1681 agaaactcgt tgagcggcag gcagttaatg gcgaccacac caagcggtcg gtggccgcgg
     1741 cgctttcgcc atgccagccg gcacctaagt agcgggcgga ccgacgcgcg ggcaggagat
     1801 ggtgcaatgg gcgacgccga ttcgagccaa ggccgaaaaa cgctgaccgc agcggccctc
     1861 gacccgcacg atcgcgcgcg attgcgcgag ctcaagcgaa ccaaatggct agccggcggg
     1921 gttctggtcg cgagcctcac ggttcttgtc atcgcgaagc tcctcgagcg ccacaacgcg
     1981 ggcttcgggt ttctcgccgc cttcacggag gcggcaacga tcggcggcct cgcggattgg
     2041 tacgccgtcg tcgtgttgtt tagacggccg ctcggtctgc cgatcccgca taccgccatc
     2101 atcccggcga accatcgcga gatcgcggaa cggctcgggg gcttcattga gacacatttt
     2161 ctcgcgcccg aaccggtcgg agcgaagctg aggcgagtcg attttgcggt ggcggcgtcg
     2221 gaatggctgc gcgatccgga acgcagcgac gggctcgcac gcttgatcct acggctgctg
     2281 ccgcaggcat tgacggcggc cgagcattca ggtctgcgcg gcttcctggc aaggcaactc
     2341 gtcgatcaga ttgaggcggt gaaactcgcg ccgctcaccg cggagctgat gaccacgttc
     2401 acgcaagacc agcgtcacca gcgcctgctc gacgaagtac tccttgccct caaccggctg
     2461 atgaccgatc cggtgacgct caacgcgatc cgccagaaga tccgcgccga gcttccgacg
     2521 ctgctcaatc tctatcgggc ggaccggttt ctgctgaaga agatcgcgac gtcggcgttc
     2581 acgtttctcg aggaagtaag ggcggacaag aaccatccac tgcgcagaga attcgaacgg
     2641 ttcgttgcgt cattcatcga taaaattgcg agctcgcccg agtacgccgc aaggctcgag
     2701 acgtttaagc gcgatctcat tgccgaccca cgcgtcggcg acctcgcgga gggcatgtgg
     2761 acgagctttc gccgccttct cgaacagaac gttcggggct cgaattcggt gcttcacgcg
     2821 catctgcggg ggcttctgat cgaagccggt cgcaagcttg ccgacgatcc gcgactgcgt
     2881 gcacacatca accgcggtat ggtggcggtg ctggagaact ttgttgagga ccataaaggc
     2941 ggcgcagcca cattcatcgc ggagcagatc aaggcctggg acatggatca cctggtccga
     3001 ctgatcgaga tcaacgtcgg aaaagatctg cagttcatcc gcttcaacgg cgcgatagtc
     3061 ggaggtctcg cagggctcgc gatctatacg gccgagatcc tggcgaagct tgcctgatac
     3121 ttggctccgt caggctattg gccgaagcgc ttctcgaccg gcatcgaccg ggagtgggtc
     3181 gaccgccggc gcacgcggct tgaccgaatt gaacccgctg cgcggccgtt tgacaccttc
     3241 gcgagggcga tgtgctggga agaactgaaa ttcagtgata gatttgcact ttacttccgc
     3301 cattcacgcc gtcggtcgcg attgtgtggc ggctgtcttg gcggccagaa acagacacgc
     3361 gctcctcgat gcgtcggggg aatgcgaggg aggacgtcat ggccggcaag ctgatcggcg
     3421 ttgcgcgggc ggcgtatcaa tatcctctcc tgatcaagca acttctgcac gcacccatgg
     3481 tgcagaaccc ggatcagcag atcgtctatc gggatctcag acggctcaag tattccgagc
     3541 ttcacgaccg gatcggccgt ctggccaact tgctcaagat gctcggcgtt cgccccggcg
     3601 acaccgtcgg cgtgatggac tgggacagca atcgatttct ggaagcattc ttcgctgttc
     3661 cgatgatggg cgccatccta cagactgtga acatcaggct gtcgccggag cagattacgt
     3721 acacgatcaa tcacgccggt gcgtcggtac ttctggtcaa cgacgagttc gtcgggctcg
     3781 tccaaggcat caaatcgcag cttgacaagg tgcggacatt cgtcgtcctc accgaccggc
     3841 ctgagcctgc gaagggtgac atcgggttcg ctggcgaata tgaggagctc ctcgcagccg
     3901 cctccgccga ctacgacttt ccggatttcg acgagaacac gcaggcgacg acgttctaca
     3961 caacagggac cactggcctt cccaagggcg tgtatttcag ccatcggcag ctcgtgctgc
     4021 ataccttggc ggagcttgcg gcgttcggaa gcgcacccga gcaaggccgc ttccaccgca
     4081 acgacgtgta tatgccgatc acgccgatgt tccacgtcca cgcctgggga ttcccgtggg
     4141 cggcgacggc ggccggcgtc aagcaggtgt atccggggcg gtacgctccc gatgtgcttc
     4201 tcaaactcat caagaccgaa ggcgtcacct tcacgcacgg cgtacccacc atactccaga
     4261 tgctgctggc cgccgcggag aaagcagatg tcgacctatc gggactgacc atggtgatag
     4321 gtggttcctc cctgtccaag gccctcgcct tgaaggctct ggacagaggt atcgacatct
     4381 atgcgggata cggcatgtcg gagacaggcc ccgtcctcac ggtggcgcat cttaaaagcc
     4441 accatttgac gggcaatccc gacgcacagg tggaaatccg gaccagggcg gggctcgccg
     4501 ttccgctggt cgatctccgt atcgtcgatc ccgacatgaa ggaaatggct cgcgacggca
     4561 aggctacggg cgagatcgtc gcgcgagctc catggctgac ccagggctat ttcaacaacc
     4621 tcgaggcatc cgagcagctg tgggccggcg gatatctgca caccaacgac atcggaaaca
     4681 tcggtcccga cggctatgta cagatcaccg atcgcatcaa ggacgtcatc aaaaccggcg
     4741 gcgaatgggt ttcctctctc gagctagagg acatcatctc gca
//
LOCUS       DATLJT010000064         3799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2300245,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000064 DATLJT010000000
VERSION     DATLJT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300245"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VM910_01330"
     CDS             complement(<1..165)
                     /locus_tag="VM910_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HVI61226.1"
                     /translation="MKESDVRARAFAMPLTSPAFPPGPYRFRQREYLIIAYRTDPDKL
                     RALVPEPLEVD"
     gene            complement(216..1346)
                     /locus_tag="VM910_01335"
     CDS             complement(216..1346)
                     /locus_tag="VM910_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI61227.1"
                     /translation="MPQGRTPFEQIALLLQGGGALGAYQAGVYEALAEADLHPDWVAG
                     ISIGAINSAIIAGNPPKQRVDALREFWHAVSTSPVGIPYFRQIEFSDHQHQLLNQARA
                     VNILLFGTPNFFVPRLPPPALWPPGSPDKASYYNNTPLRATLERLVDFERINSGEMRF
                     SVGAVEVRSGNFTYFDNTKHKIAFEHIAASGSLPPGFPATKIGDEYFWDGGLISNTPL
                     EWVLGARPRRDTLAFQVDLWHARGELPKDMIAVEVRQKEIIYSSRNRAATDRYRYIQK
                     LRIAFANLLKQMPEELRESADAKLLLAEADDKVCNIVHLIYRARPYEGIAKDFEFSRR
                     TMEEHWQTGYSNARQTLANPEALELPDRLEGVRTFDIGEERP"
     gene            1721..>3799
                     /locus_tag="VM910_01340"
     CDS             1721..>3799
                     /locus_tag="VM910_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI61228.1"
                     /translation="MGPHSLIGASSDGGSQVLWEDGERVFRRGWRPHDNGKRAVLLVM
                     PAAEHPSRSSLDRLTHEYELKDELDGAWAVRPLELVRDAGRTMLVLEDAGGEPLDRLL
                     GAPMQVVRFLRLAISVAVALGKLHQRGLVHKDIKPINILVNGTTGEVRLTGFGIASRL
                     ARERQSPHPPETIAGTLAYMAPEQTGRMNRSIDSRSDLYALGVTFYQMLTGALPFTAA
                     DPMEWVHCHLARRPATPAERLREIPGAVSAIVMKLLAKRAEDRYQTAAGLESDLRRCQ
                     TEWDALNRIDEFPLGEHDTPDRLLIPEKLYGRRREVETLLAAFDRVVSGGAPELVLVS
                     GYSGIGKSSVVYELQPVLVPPRGLFASGKFDQYKRDIPYSTLAQAFQSLIRPLLGKNE
                     ADLAPWRDGLREALGPNAGLIVDLVPEVKLIIGEPPPVPELAPQDAQRRFQLVFRNFI
                     GVFARPEHPLALFLDDLQWLDAATLDLLEDLLSRSELRNLLLIGAYRDNEITPAHPLM
                     RKLDAIKTAGGRIEEITLGPLGRAHIGQLIADTVRCTSEPIAPLTQLVHQKTGGNPFF
                     AIQFISSLADERMLAFDHDTACWYCDLDRIHAKSYTDNVVDILVAKLARLPAKTQLAL
                     RQMACLGNAAEVTMHAIVLGTSTEEVHAALSEAVRQELVERLEGSYKFIHDRVQEAAY
                     SMIPPALRAEA"
BASE COUNT          722 a         1186 c         1162 g          729 t
ORIGIN      
        1 atcgacctcc agcggctccg gcaccagcgc gcgcaactta tccggatcag tccggtaagc
       61 gatgatgaga tattcccgct gacggaagcg gtagggtcca ggcggaaacg cggggctcgt
      121 caacggcatc gcaaaagccc gtgcccgcac atcactttcc ttcatggcga ttcctctcgt
      181 gaacaatgaa gaccgaacgc gcgcacaatt cgatgtcagg gccgctcttc gccgatatcg
      241 aacgtgcgca ccccttcgag ccgatccgga agctcaagcg cctcgggatt ggccaatgtc
      301 tggcgcgcat tgctgtaacc ggtctgccag tgctcctcca tggtgcgccg ggagaactca
      361 aaatccttgg cgatgccttc atagggccgg gcgcgataaa tcagatgcac gatgttacag
      421 accttgtcgt cggcttcggc gagcaagagc ttggcgtcgg cgctctcgcg caattcttcg
      481 ggcatctgtt tgagtagatt ggcgaatgca atccgcagct tctgaatata tcgatatcga
      541 tcggtagcgg cacgattccg gctggaatag atgatctcct tttgccgtac ctcgactgcg
      601 atcatgtcct tcggcaattc gccgcgcgca tgccaaagat cgacctggaa ggcgagcgtg
      661 tcgcggcgcg ggcgtgcgcc gagcacccat tcgagcggcg tattcgagat caagccgccg
      721 tcccaaaaat actcgtcgcc gatcttggtc gccgggaaac ccggcggcag cgaacccgag
      781 gcggcgatat gttcgaaagc aatcttgtgt ttggtgttgt cgaaataggt gaaattgccg
      841 ctgcgcactt cgaccgcgcc gacgctaaag cgcatctcgc cggaattgat acgctcgaaa
      901 tcgaccagac gctcgagtgt cgcgcgcaac ggcgtgttgt tgtaatagct cgccttgtct
      961 ggactccccg gcggccaaag ggccggcggt ggcaggcgcg gcacgaagaa attcggcgtg
     1021 ccgaacaaca agatattcac ggcgcgcgcc tgattgagaa gctgatgctg gtggtcgctg
     1081 aattcgatct gcctgaaata gggtatgccg actggcgaag tagagaccgc gtgccaaaat
     1141 tcgcgcagcg cgtccacccg ttgcttcggc ggattgccgg cgatgatcgc cgagttgatg
     1201 gcgccgatcg agattccggc aacccaatcg ggatgcagat cggcctcggc gagcgcttca
     1261 taaacgcccg cctgataagc cccgagcgcg ccgccgccct gtagcaacag cgcaatttgt
     1321 tcgaacggag tgcgcccctg cggcacggca aatctctgcg ccgcgacaag cctatgacgc
     1381 ttgcgcccct gctcgcaagt tggcgcgggc gctggatgcg gccggcatcg aaggccaatg
     1441 tgttcatcaa ccatttgagc tcccattctt ccagcttgcc cgcgtcggcc tctagttgac
     1501 ccggatatgc tgtgggccca agtgaatccc ggcaaatcgc gccaaatcgt tgctaacgag
     1561 acgtcgcacc gcgtacgtct gatcaggcaa gatcaacgga cggctggtat ttgtcaggcc
     1621 tgatgcggta cgatttctgc ggagggggaa tcgcgggcgc gcagtgctgg taagctgggt
     1681 gagccgaact caacccgaag cccgacgccg gagttgacag atggggccgc attcgctgat
     1741 cggcgcatcc tcagatggcg gctctcaggt cttgtgggag gacggcgaac gcgtcttccg
     1801 ccgtggctgg cgaccgcacg ataacggcaa gcgcgccgtg ttgctcgtta tgcctgccgc
     1861 ggagcacccg tctcgctcaa gcctcgatcg cctcacccac gaatacgaac tcaaggatga
     1921 gctcgacggg gcgtgggcgg tgcgaccgct cgagctcgtg cgcgatgccg gtcggaccat
     1981 gctggtgctc gaggacgccg gcggcgagcc gctcgaccga ttgctcggcg ctcccatgca
     2041 ggtggtacgc ttcttgcgct tggccatcag tgttgccgtg gctctcggca agctccacca
     2101 gcgcgggctc gtccacaagg acatcaaacc gatcaatatc ctggtcaacg gtacgaccgg
     2161 cgaggtgcgg ctgaccgggt tcggcatcgc ctcacgcttg gcgcgtgagc gccagtcgcc
     2221 tcatcctccg gagacgatcg ccggcacgct cgcctacatg gcgcccgagc agaccggacg
     2281 gatgaatcgg tcgatcgatt cccgcagcga cctctacgcc ctcggcgtca ccttctacca
     2341 gatgctcacc ggtgcattgc cgtttaccgc ggctgatccc atggaatggg tgcactgcca
     2401 cctcgccagg cgcccggcga cgccagccga gcgactgagg gaaatccccg gcgccgtctc
     2461 ggcgatcgtc atgaagctgc tcgcgaagcg ggccgaagat cgctatcaga ccgccgccgg
     2521 cctcgaaagc gaccttcgcc gctgccagac cgaatgggac gcgctgaacc ggattgacga
     2581 gtttccactc ggcgaacacg acacgcccga ccggctgctg atccccgaga aactctacgg
     2641 acgccgacgc gaggtcgaga ccttgctcgc cgctttcgat cgcgtcgtga gcggtggtgc
     2701 cccggaattg gtgctggtct cgggctattc cggcatcggc aagtcctcgg tcgtctacga
     2761 gttgcagccg gtgctggtcc cgccgcgcgg ccttttcgcc tccggcaagt tcgatcagta
     2821 caaacgcgac attccctact cgacgctggc acaggccttt caaagcctga ttcggccgtt
     2881 gctcggcaaa aacgaagcgg accttgcgcc ttggcgcgac gggttgcggg aggcgctggg
     2941 accgaacgca ggattgattg tggatctcgt tcccgaagtg aagctgatca tcggagagcc
     3001 gccgccagtt cctgagcttg caccgcagga cgcgcaacgg cgcttccagt tggtatttcg
     3061 gaatttcata ggcgtcttcg cgcggccgga acatccgttg gcgctgttcc ttgacgactt
     3121 gcaatggctc gacgcagcga cgctcgacct gctggaagac ctgctgagcc ggtccgaact
     3181 tcgcaatctt ctcctcatcg gcgcctaccg cgacaacgaa atcactccag cgcatccgct
     3241 catgcgcaag ctcgatgcga tcaagaccgc tggcggaaga atagaggaga ttacgcttgg
     3301 gccgctcggc cgagcacata tcgggcagtt gattgcggac actgttcgct gcacgtctga
     3361 acctatcgcg ccgctcacgc aactggtgca ccagaagacg ggtggcaatc cgttcttcgc
     3421 cattcagttc atttcttcgc tcgccgacga aagaatgctc gcctttgatc atgatactgc
     3481 gtgctggtac tgcgatctcg atcgcattca cgccaagagt tacaccgata atgtggtgga
     3541 catcctggtc gcgaagctgg cccgcctgcc ggccaaaacg cagctggcgt tgcggcagat
     3601 ggcctgtctc ggcaatgccg ccgaagtcac gatgcatgcg atcgttctcg ggacgtctac
     3661 ggaggaggtc cacgcggcgc tatcggaagc ggtccgtcag gaattggtcg agcgcctgga
     3721 gggttcctac aagttcatcc acgaccgggt gcaggaagcc gcttactcaa tgataccacc
     3781 agcactgcgt gcggaggcc
//
LOCUS       DATLJT010000065         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381163,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000065 DATLJT010000000
VERSION     DATLJT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381163"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1051)
                     /locus_tag="VM910_01345"
     CDS             complement(59..1051)
                     /locus_tag="VM910_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61229.1"
                     /translation="MIKRREFITLLGGAAVAWPLAVRAQPTTERVRRIGVLVSTTADD
                     PEGQARIAAFHQGLQERGWTLGRNARVDVRWGAFDADSSRHYATELVALAPEVIVASA
                     SAAMGALQQMRTVPIVFVSIIDPVGAGFVESLARPGGNVTGFSLFEYGLSGKWLELLK
                     EIAPSVTRAAVLREAALASGVGQYAIIQAVASSLGIELRPIDLRDPGEIERGVLAFAR
                     EPNSGLIIVGAPSGVVHRDLIIALAARHRLPAVYPYPAFAHSGGLISYGPDAVDQYRR
                     AAGYVDRILKGAKPADLPVQAPTKYELVINLKTAKALGLEIPSSVLARADEVIE"
     gene            complement(1048..2034)
                     /locus_tag="VM910_01350"
     CDS             complement(1048..2034)
                     /locus_tag="VM910_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61230.1"
                     /translation="MKRREFITLLASTTATWPLVARTEQAIPVIGFFHVASLETRRDY
                     LPWFNKGLGEAGYTDGRNVRIEYRWAHGQSDQLPRLAAELVRQNVSVLVILESTHGAL
                     SAKAATQAIPIVFMQGADPVETGLTRSLNHPGGNVTGFTLLMSGTVGKRLELLHELVP
                     AAKVIGYLRNPTNPAYAKAETEAMQAAARALGIRLLLVDANQPAEIENAFGNFVQQKA
                     AALLVSADALLTTTHADQIVALAGHHAIPTSYLLGEIVSAGGLMSYGPDLFDAWRQAG
                     VYTGRILKGEAAGDLPVQQVTKLRLVINLKTAKALGLTVPDKLLALADEVIE"
     gene            complement(2031..3014)
                     /locus_tag="VM910_01355"
     CDS             complement(2031..3014)
                     /locus_tag="VM910_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61231.1"
                     /translation="MIKRREFITLLGGAAAAWPLAVRAQQPAMPVIGYLSFGSPESDA
                     PRLTGLRRGLNETGYVEGRNFVIEYRWAGNQVDRLPALAADLVQLQVTVIVAAGVPPA
                     LAAKAATTRIPIVFSVAADPVQLGLVASLNRPGGNLTGFNGFVGELGAKGLALLHELV
                     PSTATIGFLENLNNPLFELTTRDVLAAAPVLGLKVQTLKAGTDREIDAAFASLVQART
                     GALLVGSDLFFNNRIEQIIGLAARHAIPTMFTFREFVLAGGLISYGISLIENYRQVGL
                     YAGRILKGEKPADLPVIQATKLELVINLKTAKALGLQIPDRLLALADEVIE"
     gene            complement(3011..3751)
                     /locus_tag="VM910_01360"
     CDS             complement(3011..3751)
                     /locus_tag="VM910_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61232.1"
                     /translation="MAKELADLRPDATLGQTTPVVRALAREMPASPIVFVTVSDPIGS
                     GFAASLARPGGNITGFTVDDSALGGKWVELLKQIAPTTARVMLLFNPAGPPLQFFLPS
                     IQAAASSLSVQANVVTIHTKDEIEGVIAEQAREPGSGIVVLANAFNTTNRELIIELTA
                     RYRVPAIYFSAYCAQSGGLIGYGSDYAEQARQAAGYVDRILRGTKPADLPVQAPTKFE
                     LVINRKTAKTLNLDVPDKLLALADEVIE"
     gene            complement(3987..>4439)
                     /locus_tag="VM910_01365"
     CDS             complement(3987..>4439)
                     /locus_tag="VM910_01365"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61233.1"
                     /translation="ESEARDLEEAARAIGHQILMVNAANEREVHAAFAKVVQAGAGGL
                     LIGTSPFFLSQRRQLVALAARHALPTMYNQREYAEVGGLISYGPSQSDAYRRAGAYVG
                     RILKGEKPGDLPVDVGAKFELVINLATAKAFGVEIPPTLLALADEVIE"
BASE COUNT          858 a         1387 c         1350 g          844 t
ORIGIN      
        1 cgcgccgcga gcggccacgc cgccgcgccg ccgagcagcg tgatgaactc gcggcgtctc
       61 actcgatcac ctcgtcggcg cgcgcaagca cggacgacgg aatttcgagc ccgagcgcct
      121 tggcggtctt gaggttgatc accagttcgt acttggtcgg cgcctgcacc ggaagatcag
      181 ccggcttcgc gcccttgagg atacggtcga catagccggc ggcgcgtcgg tactggtcga
      241 ccgcatctgg cccgtaggag atcagaccgc cgctgtgagc gaaagccggg taggggtaca
      301 cagcaggcaa tcggtggcgg gcggcgagcg cgatgatgag atcgcgatga accacccctg
      361 atggagctcc aacgatgatc agaccactgt tcggctcgcg cgcgaacgcc aggacgccgc
      421 gctcgatctc gccgggatcg cgcaggtcga tcggacgcaa ctcgatcccg agcgacgacg
      481 ctaccgcctg gatgatggca tattgaccga ccccggaggc taacgcggcc tcccgaagga
      541 ccgccgcccg cgtcacgctg ggcgcgatct ctttgagcag ctcaagccac tttccgctca
      601 ggccgtactc gaacaagctg aatccggtga cgttgccgcc cggacgcgcc aggctctcga
      661 cgaatcccgc gccgaccgga tcgataatac tcacgaatac aatcggcacc gtgcgcatct
      721 gttggagcgc tcccatcgca gcgctcgcgg aggcaacaat gacttcaggc gcgagcgcga
      781 caagctccgt cgcgtaatgg cggctgctgt cagcatcgaa cgcgccccag cgaacgtcga
      841 cccgcgcgtt gcgtccgagt gtccatcccc gctcctgtaa tccctgatgg aacgctgcga
      901 tgcgggcttg tccttctgga tcgtcggcag tggtgctcac gagaacgcca atgcgccgca
      961 cccgctcggt cgtcggctgc gcgcggaccg cgagcggcca cgcgaccgcc gcgccgccga
     1021 gcagcgtgat gaactcgcgg cgtttgatca ttcgatcacc tcgtcggcga gggcgagcag
     1081 cttatccggc acagtaaggc cgagcgcctt ggcggttttc aagttgatca cgagccggag
     1141 ctttgtgact tgctgcacgg gcaaatcgcc agccgcctcg cccttcagaa tacgcccagt
     1201 gtagacaccc gcttgccgcc aggcatcaaa caggtccggt ccataactca tgaggccgcc
     1261 agccgagaca atctccccca gaaggtacga ggttgggatt gcatggtggc ccgcgagcgc
     1321 gacgatctga tctgcgtgag tagtggtaag taaggcgtcg gcggacacta gaagcgccgc
     1381 ggccttctgc tgaacaaaat tgccaaatgc gttctcgatc tccgctggct gatttgcgtc
     1441 aacgagcagc aaacgaatcc caagggcacg cgccgcggct tgcatcgcct ccgtttcggc
     1501 cttcgcataa gccgggtttg tcggattgcg gagataacca atcaccttag ccgcaggcac
     1561 taattcgtgc agcagttcaa gccgctttcc aaccgttccg ctcatcagaa gagtaaaccc
     1621 tgtgacgttc ccgccgggat ggttgagact gcgagttaag ccggtctcga ccgggtccgc
     1681 gccttgcata aagacaatag ggatggcttg ggtggccgcc ttggcggaaa gcgcaccgtg
     1741 tgtgctctcc agtatgacga gaacagatac gttctggcgc accaattcag cggctaatct
     1801 tggcagctga tcactttggc catgtgccca tcggtactcg atcctcacat ttctgccgtc
     1861 ggtgtaacca gcctctccga gacctttgtt aaaccagggg aggtaatcac gcctcgtttc
     1921 caacgatgca acgtgaaaaa acccgatcac gggaatcgct tgctccgtgc gtgccacgag
     1981 cggccacgta gccgttgtgc ttgcaagcag tgtgatgaac tcacgccgct tcactcgatc
     2041 acctcgtcgg caagcgcgag cagcctgtca ggaatttgga ggccgagtgc cttcgcggtc
     2101 ttgaggttga tgactaactc gagcttcgtg gcctgtatga ccggcaggtc ggcgggcttt
     2161 tcacccttaa ggatacgccc cgcgtaaagt ccgacctgtc ggtaattctc tatcaggctg
     2221 attccgtaac tgatgagccc gccagccagg acaaactcac gaaacgtgaa catggtcgga
     2281 attgcatggc gcgccgcgag tccaatgatt tgctcaatcc ggttgttgaa aaaaagatca
     2341 ctaccgacga gcagcgcccc ggttcgcgct tggaccagac tcgcgaaggc ggcatcgatt
     2401 tcgcggtcgg tgcctgcttt caatgtctga acctttagcc cgaggactgg agccgccgcc
     2461 agcacgtctc tggtcgttag ctcaaatagc ggattgttaa gattctcaag aaagccgatc
     2521 gtcgcagtgc tgggcactag ctcgtgcaaa agcgcgagtc ccttggcccc gagttcaccg
     2581 acgaagccgt tgaagcccgt caggttgcct cccggccgat taaggctggc aacaagcccg
     2641 agctgcacag ggtcagcggc gacggagaac acgatgggga tgcgggtagt cgcagccttg
     2701 gcggcgagag caggaggaac tccggctgcg acgatcacag tgacttgaag ttggaccaga
     2761 tcagccgcca acgctggcag tcggtcaact tggtttcccg cccagcgata ttcgattaca
     2821 aagttccggc cttcaacgta gccggtctcg ttcaaacccc gccggaggcc ggtgagccgg
     2881 ggggcgtcgg actccggcga cccgaagctt aaatacccaa tcaccggcat cgccggctgc
     2941 tgcgcgcgca ccgcgagcgg ccacgcggct gctgcgccgc caagcagcgt gatgaactcg
     3001 cggcgtttga tcattcgatc acctcgtcgg cgagcgcgag cagcttatcc ggcacgtcga
     3061 ggttgagcgt ctttgcggtc ttgcgattaa tcaccagctc aaacttggtt ggcgcttgca
     3121 ccgggaggtc cgcgggcttg gtccctctga ggatgcggtc aacgtatccc gccgcttgtc
     3181 gcgcctgttc ggcataatca ctgccatagc caatcaagcc gcctgattgc gcgcaatacg
     3241 ccgagaaata gatcgccgga acgcggtaac gggctgtcag ctcgatgatc agttcccgat
     3301 tggtggtgtt gaaagcgttc gccagcacga caatgccgct ccctggctcg cgcgcttgct
     3361 ccgcgataac gccttcgatc tcatccttcg tatgaatcgt aacgacgttc gcctggacgc
     3421 ttaaggagga tgcggcggct tgaattgaag gcaagaagaa ttggagtggc gggccagccg
     3481 gattgaacag cagcatcact cgggcagtgg ttggcgcaat ctgcttcaac agttcgaccc
     3541 atttgccgcc cagtgcggaa tcgtcaaccg tgaagccggt gatattgccg cccggacgcg
     3601 cgaggcttgc ggcgaagccg ctgccgattg gatcggatac tgtgacaaat acgataggac
     3661 tggccggcat ctcgcgggca agggctctga ccacgggcgt cgtctgaccg agggtcgcgt
     3721 cgggccggag atcagccaac tcttttgcca tcgtgttaat cttatcggga tcagggccag
     3781 cccagcgaag ttcgatgcgg acattgctcc cttcagtcca tccgagcttc catttcggaa
     3841 ggctgcaacc atagactgcg caaccggatc actccccgcg aatcccatca acacgccgac
     3901 cagtcgcgtc cggttcggga attgtgcgtg cgccgcgagc ggccacgccg ccgcaccgcc
     3961 gagcagcgtt atgaactcgc ggcgtttcac tcgatcacct cgtcggcgag tgcgagcagc
     4021 gtcggtggga tttcgacgcc gaatgccttt gcggtggcga gattgatgac cagctcgaat
     4081 ttggccccaa cgtcgaccgg cagatcgccg ggcttttcgc ctttgagaat ccggccgacg
     4141 taggccccgg cgcggcgata agcgtccgac tgactaggtc cgtagctgat caagccgcca
     4201 acctcggcgt actcacgctg attatacatc gtcggcaggg catgccgggc agcgagcgcc
     4261 acgagctgtc ggcgctggct gaggaaaaat ggactggtgc cgatgagcag cccgcctgcg
     4321 cccgcttgca cgacctttgc gaaggcggca tggacttctc gctcgttggc cgcgttcacc
     4381 atcaggattt ggtgcccgat ggcacgggcc gcttcctcca ggtctcgcgc ctcgctctc
//
LOCUS       DATLJT010000066         1761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4138278,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000066 DATLJT010000000
VERSION     DATLJT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138278"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VM910_01370"
     CDS             complement(<1..861)
                     /locus_tag="VM910_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61234.1"
                     /translation="MKGCRIEIALKARPRMSLLVAAAVTLSGAFFSAQAQEPIKIGFG
                     VSLTGGLASSGKAHLMSKQIWAEEINAKGGLLGRPIKLVYYDDQTNAATVPGIYAKLI
                     DVDKVDLLMGHATNLIVAAMPLIIERKKLVMVLLALGSNAEFKYPRYFQSAAFGPDSK
                     GVLSNNFFAVAKSLAPEPKTVALVGADAEFSNNVLIGARENAKKYNLKIVYDRSYPPA
                     TVDYTPIVRAIQATSPDVVLVASYPPDSVGIVRAATEIGLKTQLFGGAMVGMQYASLM
                     QQLGEKLNRVV"
     gene            1396..>1761
                     /gene="coaD"
                     /locus_tag="VM910_01375"
     CDS             1396..>1761
                     /gene="coaD"
                     /locus_tag="VM910_01375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVI61235.1"
                     /translation="MPRTALFPGSFDPVTNGHLDLVRQAARLADRLVLAIGTHPGKAP
                     LFEVEERRAMIEETCGALARASGCDFACITFGDLVVAAAQREGATLLIRGLRSATDFD
                     YEIEMAGMNATMAPAVQTVF"
BASE COUNT          310 a          562 c          549 g          340 t
ORIGIN      
        1 cacgacgcgg ttgagctttt cgcccagctg ctgcatgagc gacgcgtatt gcattccgac
       61 cattgcccca ccgaacaatt gggtcttcag gccgatttcg gtcgccgccc gaacgatccc
      121 gacggaatcg ggcgggtagg acgcaaccag aaccacgtcg ggactggtcg cctggatggc
      181 gcgtacgatc ggcgtgtagt cgaccgtggc cggcggatag ctgcggtcgt atacgatttt
      241 gaggttgtac tttttcgcgt tctctcgcgc gccaatcagc acgttgttgg agaactcggc
      301 gtcggcgccg accagggcca cggtcttcgg ctccggcgcg agcgatttgg cgaccgcgaa
      361 gaagttgttc gacagcacgc ccttggaatc agggccaaag gctgcgctct ggaaatagcg
      421 cggatacttg aactccgcat tagagcccag cgcgaggagc accatgacca gcttcttgcg
      481 ctcgatgatg agcggcatcg ccgcgacgat gaggttcgtc gcgtggccca tcagcaggtc
      541 gaccttatcg acgtcgatca gcttggcgta gatgccgggt acggtcgcgg cgttggtctg
      601 gtcgtcgtag tagacgagct tgatcggacg gccgagcagc ccgcccttcg cgttgatctc
      661 ctcggcccag atctgtttgg acatcagatg ggccttgccc gaggaggcga gcccgccggt
      721 gagcgatacg ccaaagccga tcttgatcgg ctcctgcgcc tgcgccgaaa agaacgcgcc
      781 ggacaatgtc accgccgccg ccaccagtag cgacattcgc ggccgtgctt tcagcgcgat
      841 ctcgatcctg caccctttca tcgcgtcctc ccctggtgca tcaaacgagc caacgcaccg
      901 tgcgttcgat gccacgatac tagcgatttg ctgagtgctc gaaagccaag atgcgagatg
      961 ggaccgtgcc cgccgttgca acgggccgcg aactgggtgg gcgccgcaca atcgaataat
     1021 accgtttgca ttttcgccgg cttgctccgc cttcatcgag cagcggcatc cgatttctct
     1081 tgagttgggc aaggtgggtc ttgaggctgg cctccaggcc gcaacgcgac gagtaccggg
     1141 catccccgta ccgtcagtaa tgcgagcccg ttgctgcaac gccgggcgcc aagatcgtgc
     1201 ggcaaccctt aagccgcgca cgagggaagc agttcataat tctgattcag taaaaggcgt
     1261 aagaatctga gggacagggc cattggtaaa ccattcggcc tgggttcgtg ccacccctga
     1321 tagcgtcccc ccgccagttc ggatggccgt ctgcacgacc ttgcggctcc ctggggcgcg
     1381 cgctagaccc cctgcatgcc gcggaccgcg ctttttcccg ggtcgttcga tcccgtcacc
     1441 aacggccatc tcgatctcgt gcgccaagca gcccggctcg cggaccggct cgtactcgcg
     1501 atcggcactc atcccggcaa ggcacccttg ttcgaggtcg aggagcgccg cgccatgatc
     1561 gaggagacct gcggcgcgct cgcgcgcgcc agcggttgtg atttcgcctg catcaccttt
     1621 ggcgatctgg tcgtggctgc ggcccaacgc gagggcgcga cgctcttgat ccgcgggctg
     1681 cgcagcgcga ccgacttcga ttacgagata gaaatggcgg gcatgaacgc caccatggcg
     1741 cccgccgtgc agacggtttt c
//
LOCUS       DATLJT010000067         5075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2759802,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000067 DATLJT010000000
VERSION     DATLJT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759802"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VM910_01380"
     CDS             complement(<1..256)
                     /locus_tag="VM910_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI61236.1"
                     /translation="MKAILCKQYCQPDDLKLEEVPDPVAQPGEAVIAIKAAALNFFDI
                     LMIQGKYQIKPPFPFSPAAEVAGVVESVGAGVTDLKPGDRV"
     gene            370..1209
                     /locus_tag="VM910_01385"
     CDS             370..1209
                     /locus_tag="VM910_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI61237.1"
                     /translation="MFENGLLKGRRVLVTGGGSGLGAAMGRRFLELGADLLICGRRLE
                     LLEQAAAEMRSATSGKVAIHRCDIRDGAQVDAMMHGIFAEAQIDVLVNNAAATFIAQT
                     EHLSFRAADAILAPTLHGAMYCTLAAGRRWIEGKRKGVVLSILSTSTITGRAFTVPSA
                     MAKSALLAMTKSLAVEWGPKGVRLVAIAPGAFPTPGATGQLRPQGRDEGWAARNPLGR
                     AGEHRELADLASFLVSDGAAYINGEMVVVDGGAHLRTSGAEDLLQWSNEQWENQRASR
                     AKG"
     gene            1339..1938
                     /locus_tag="VM910_01390"
     CDS             1339..1938
                     /locus_tag="VM910_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI61238.1"
                     /translation="MFRWILTALVTGILACVVAGFAHAQSAPPKPAKAAAKQETKPPA
                     AAIAGGAEPTLIGQYGTWGAYSATPNGRRVCFALAKPTSSKTNPPNRPRDPAYAFVST
                     RPSEKVINEVSVMIGYALKPGSESTLEVGGAAYAMYTQGDGLWIKNAAEEEQMVQAMR
                     RSADAVVKGVSAKGTETTDTFSLKGLGQALDRIAQDCKR"
     gene            2032..3225
                     /gene="rlmN"
                     /locus_tag="VM910_01395"
     CDS             2032..3225
                     /gene="rlmN"
                     /locus_tag="VM910_01395"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HVI61239.1"
                     /translation="MSGQQASAPLEKTPLEIYVPPARPSLIGLSRAELGECLAEIGVG
                     PAQRKMRVQQLWHWIYFRGARNFDEMTSVSKETRARLAERFTVDRPEVVAEQVSNDGT
                     RKWLLRLPSGETHEKPHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRLVRNL
                     TAGEIVGQVMVARDRLNDWADRENGSRLVTNIVMMGMGEPLYNFEAVRDALLIVADNE
                     GIGISRRRITLSTSGVVPNIKRTGEEIGVMLAISLHAVRDELRNELVPLNRKYPIAEL
                     LQACRDYPGASNARRITFEYVMLKGVNDSLEDAKLLVKLLKGIPAKINLIPFNPWPGT
                     RYECSDWDQIEKFSEYIFNAGYSSPVRTPRGRDILAACGQLKSETEKLSARERQALRA
                     MAMTD"
     gene            3249..3737
                     /locus_tag="VM910_01400"
     CDS             3249..3737
                     /locus_tag="VM910_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61240.1"
                     /translation="MALIGRLFVIFFGFLAACLVAGIIVVAAVLFPELGGLGAGPIDQ
                     SAFDIVLGFSFIFISGFALLPAMIIAAITEAFCIRGVLAYAVGGGLVGLACYLGLVPF
                     DPDTLHFEGIVRRHLEIMTGAGIVAGLVYWMIAGRNAGAWRAPAIRPPPPLPSQSRPP
                     RV"
     gene            3782..4558
                     /locus_tag="VM910_01405"
     CDS             3782..4558
                     /locus_tag="VM910_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVI61241.1"
                     /translation="MNRTGLFVALSLALTIGIVFGLYPELDLKLAALFYDVPTRSFPL
                     KLVERAAFARDAAMWIAWAIAVPAIAALLIKLLRPDRPLLMSGRAIIFLLLTLTLSAG
                     VLTNLTFKSYWGRPRPVLVTEFGGTLPAVAWWDPHGGCGRNCSFFSGESATAFWTLAP
                     AALTPPAWRPLAYAAATLFGLATSVLRMAFGGHFFTDIAAAGLMTYLVIWLVHAFIYR
                     RPRTRLSDAGIDAALTRLFWPAYRLRQRWRGREAGPAPSM"
     gene            4660..>5075
                     /locus_tag="VM910_01410"
     CDS             4660..>5075
                     /locus_tag="VM910_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HVI61242.1"
                     /translation="MSTILKGLPKGEKVGIAFSGGLDTSAALLWMKQKGARVFAYTAN
                     LGQPDETDYDEIPRKALEFGAEKARLVDCRTQLVHEGIAAVQSGAFHVSTAGATYFNT
                     TPLGRAVTGTMLVSAMKEDGVNVWGDGSTFKGNDIER"
BASE COUNT          872 a         1624 c         1640 g          939 t
ORIGIN      
        1 ccacgcggtc gccgggcttg aggtccgtga cgcctgcgcc gacgctctcg acgacgcccg
       61 cgacctccgc agccggcgaa aacggaaacg gcggcttgat ctggtacttg ccctggatca
      121 tcaggatgtc gaagaagttc aacgcggccg ccttgatcgc gatcacggcc tcgcccggct
      181 gggccacggg gtccggcact tcctccagct tgaggtcgtc gggttggcaa tattgcttgc
      241 agaggatggc tttcatggac gcacctgatg ttcggacgcg atagcctgag gtttaacgct
      301 tcatgccgga tttgaagctg cgccacaata tatccgggga gaagcaaaaa atccgggagg
      361 attcaaacga tgtttgaaaa cggtctactc aagggcaggc gcgtcctcgt cacgggcggc
      421 ggcagtgggc ttggggcggc gatgggacgg cgttttctcg agctaggcgc cgatctcctg
      481 atctgcggcc gccggcttga attgctcgag caggcggcgg ccgagatgcg cagtgccacc
      541 tccggcaagg tcgcgatcca ccgttgcgac atcagggatg gcgcacaggt cgatgccatg
      601 atgcacggca tttttgccga ggcgcagatc gatgtactcg tcaacaatgc cgccgcgacc
      661 tttatcgcgc aaaccgagca tctgtctttt cgtgccgccg acgccattct cgcgccgacc
      721 ctgcacggcg cgatgtattg cacgctcgcg gccggcagac gctggatcga gggcaaacga
      781 aagggcgtag tgttgagcat tctgtcgacc tcgacgatta ccgggcgcgc tttcacggta
      841 ccctcggcga tggccaaatc cgcattgctg gcgatgacca aatcgctcgc ggtcgagtgg
      901 ggaccaaaag gggtccgcct ggtcgcgatt gcgcccggcg cgtttccgac cccgggcgcc
      961 accggtcagc tacgccccca aggccgtgac gagggctggg cggcacgcaa tccgcttggc
     1021 cgcgccggcg agcatcggga acttgccgat ctcgcaagct tcctggtttc cgacggcgcc
     1081 gcctacatca atggcgagat ggtggtggtg gatggcggcg cgcatttgcg cacctcgggc
     1141 gccgaggatc tcctgcaatg gagcaacgaa cagtgggaga accagcgggc atcgcgggcc
     1201 aagggctgaa ctcgatcaag ggctgaactt gatgaaggct gaccgtcttt cggtggcgcg
     1261 cttcccgttg agcagaggtt ggtgacggag cgcggcgggc catcttccag tcacaatatt
     1321 gcggaaccaa atcccgtcat gtttcggtgg attctcacag cgctggttac gggcattctg
     1381 gcgtgcgtgg tggcgggctt tgcgcacgcc cagagcgcac cgccgaagcc ggccaaagcc
     1441 gccgccaagc aggagaccaa gccgccggct gcggcaatcg ccggcggcgc cgagccgacc
     1501 ctgatcggcc agtacggcac ctggggcgcc tatagcgcaa cgccgaacgg caggcgcgtc
     1561 tgttttgccc tggcaaagcc cacctcgtcc aagaccaacc cgccgaatcg cccgcgcgat
     1621 ccggcttacg ccttcgtcag cacacgtccg tccgaaaagg tgatcaacga agtctccgtg
     1681 atgatcggtt acgcgctgaa gccgggctcg gaatcgacgc tggaagtcgg cggcgccgct
     1741 tatgcgatgt atacgcaggg cgatggtctc tggatcaaga acgccgccga ggaagagcag
     1801 atggtccagg ccatgcgccg ctcggccgac gccgtggtga aaggcgtctc ggccaagggc
     1861 accgagacca ccgatacctt ctcgctgaag gggttggggc aggcgctcga ccggatcgcg
     1921 caggactgca agcgctaagc ggctttcaat accgtcatcg acgcatttga tgcctttcca
     1981 gcccgcacgg taaggcttat ctgatggccc atgacagaca ccctgaccag catttccggg
     2041 cagcaggcca gcgcgccgct ggagaagacc ccgcttgaga tctatgtgcc gccggccaga
     2101 ccgtcgctga ttgggctgtc gcgcgccgag ctcggcgagt gcctggcgga aatcggcgtg
     2161 gggccggcgc agcgcaagat gcgcgtgcag caactgtggc actggatcta ttttcgcggc
     2221 gcgaggaatt tcgacgagat gacctccgtc tccaaggaga cgcgcgcaag gcttgccgag
     2281 cgattcaccg tcgatcgtcc cgaagtggtc gccgagcagg tctccaacga cggtacccgg
     2341 aaatggctgc tgcggctgcc gagcggcgag actcacgaaa agccgcatga ggtcgagtgc
     2401 gtctatattc ccgagaccga ccgcggcacg ctgtgcgtct cctcgcaggt cggctgcacg
     2461 ctgaactgct cgttctgcca caccggcacg cagcgactgg tacgcaacct caccgcaggc
     2521 gagatcgtcg gccaggtgat ggtggcgcgc gaccggctga acgattgggc cgaccgcgag
     2581 aacggcagtc gcctcgtcac caacatcgtg atgatgggca tgggcgagcc gctctacaat
     2641 ttcgaggcgg tgcgtgacgc gctcctgatc gttgccgaca atgagggcat cggcatctca
     2701 cgccgccgca tcacgctctc gacctcgggc gtggtgccga atatcaagcg cacgggggaa
     2761 gagatcggcg tgatgcttgc gatttcgctg catgcggtgc gcgacgaatt gcgcaacgag
     2821 ctcgtgccgc tcaaccgcaa atatccgatc gccgaattgc tgcaggcctg ccgcgactat
     2881 cccggcgcgt cgaatgcgcg gcgcatcacc ttcgaatatg tgatgctgaa gggcgtcaac
     2941 gattcgctgg aggatgcaaa gcttctggtc aagctgctca agggcatccc ggccaagatc
     3001 aacctcattc cgttcaatcc atggcccggc acgcgttacg aatgttcgga ctgggatcag
     3061 atcgagaaat tttcggagta tattttcaac gccggctact cctcgccggt gcgaacgccg
     3121 cgcggccgcg atattctcgc cgcctgcggg caattgaaat cggaaaccga aaaattgtcc
     3181 gcgcgcgagc ggcaggcgct gcgcgccatg gcgatgacgg attagatgca aacctgccag
     3241 gcatcgtcat ggccctgatc ggtcggctgt tcgtgatctt cttcggcttc ctggcggcgt
     3301 gcctggtggc ggggatcatc gtggtcgccg cggtgctgtt tccggaactc ggcggtctcg
     3361 gcgcagggcc gatcgatcag agcgcgttcg acatcgtgct cggattcagc ttcatcttca
     3421 tctcgggctt tgcgttgctg ccggcgatga tcatcgccgc gatcaccgag gccttctgta
     3481 tccgcggtgt gctcgcctat gccgtgggcg gcggcctggt cgggctcgcc tgctatctcg
     3541 gactggtgcc gttcgatccc gacacgctgc acttcgaagg catcgtgcgg cgccatctcg
     3601 agatcatgac gggcgccggt atcgtcgccg gccttgtcta ctggatgatc gccggacgca
     3661 atgccggcgc ctggcgcgcg ccggcgatcc ggcctccgcc gccactgccg tcgcaatcgc
     3721 gtccgccgcg ggtgtgagtc gggggtttcc atccgcccct cgctccgcta aaccgcccgc
     3781 catgaaccgg accggactct ttgttgcgct ctcgctcgcg ctcacgatcg ggatcgtgtt
     3841 cgggctctat cccgagctcg acctgaaact ggccgcgttg ttctacgatg tgccgacgcg
     3901 gtcattcccg ctgaagctcg tcgagcgggc cgcctttgcg cgcgatgcgg cgatgtggat
     3961 cgcctgggcg atcgcggtcc cggcgatcgc agccttgctg atcaagctgc tgcgacctga
     4021 ccgcccgctc ctgatgtcgg gccgcgccat tatcttcctc ctgctgacgc tgaccctgtc
     4081 ggcgggggtc ctcaccaatc tcacattcaa aagctattgg ggccggccgc gccccgtgct
     4141 ggtgaccgag ttcggcggga ccttgccggc cgtcgcgtgg tgggatccac atggcggctg
     4201 cggtcgcaac tgctcgttct tctccggcga gagcgcaacc gcgttctgga ccttggcgcc
     4261 agccgcgctg acgcctccgg cgtggcgtcc gctagcgtac gccgcggcga ccttgtttgg
     4321 cctcgcgacc agcgtgctgc gcatggcgtt cggcgggcac ttcttcaccg acatcgccgc
     4381 cgccggcctc atgacgtatc tcgtgatctg gcttgtgcat gcgttcattt accgcaggcc
     4441 gcgcacccgt ctgtcggatg ccggcatcga tgccgcgctg acgcggctgt tctggcccgc
     4501 ctatcgcctc cgacaacgct ggcggggccg cgaggcaggg ccggcgccgt cgatgtgagc
     4561 gcgccagaag ccattaatcg cgtgcccgcc ggcgtgaaat ttgatattcg cgcacccaaa
     4621 cagcaaacga cttcgcaaat tcgaccgatt ggaagctcca tgagtacgat cctgaaaggc
     4681 ctgcccaagg gggagaaagt cggcatcgcc ttttcgggcg gtctcgacac cagcgcagcg
     4741 ctcctctgga tgaagcaaaa gggcgcccgc gtttttgcct ataccgccaa tctcggccag
     4801 cctgacgaga ccgattacga cgagattccg cgcaaagcgc tcgagttcgg cgccgaaaag
     4861 gcccggctgg tggattgccg cacgcaattg gttcacgagg gcatcgctgc cgtccagtcg
     4921 ggcgccttcc acgtctcgac cgccggcgcc acgtacttca acaccacgcc gctcggtcgc
     4981 gcggtgacgg gcacgatgct ggtctcggcg atgaaggagg acggcgtcaa cgtctggggt
     5041 gacggctcga ccttcaaagg caacgacatc gagcg
//
LOCUS       DATLJT010000068         3598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_921899,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000068 DATLJT010000000
VERSION     DATLJT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921899"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VM910_01415"
     CDS             complement(<1..292)
                     /locus_tag="VM910_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61243.1"
                     /translation="MLSAVTFSALIIGIDNLRRLSLAFAFELACAAVAGALFVWRQRM
                     LADPMLALELFARPVFALSVVGSICSFTAQNLTFVSLPFFLEEVLGRSAVETG"
     gene            complement(498..1295)
                     /locus_tag="VM910_01420"
     CDS             complement(498..1295)
                     /locus_tag="VM910_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HVI61244.1"
                     /translation="MGAVTKIEWCHHTFNPWWGCTQVSPLCDRCYAMMLDTRWFKRVH
                     WGPRASRRYFGDAYWREPLKWDCLAGAARRRHRVFCASMADVFDNEVEPAVRDRLWRL
                     IRRTRNLDWLLLTKRIGNAAEMLPNDWGGGYPNVWLVISVDQAELGRDVPKLLTIPAV
                     VHGVSIEPQLAPVRLGRFASLLQWVINGGEAGAGSRPFHLEWARALVAECRSASIPIF
                     VQRLGSKPFEGTTRLQLNDYAGGDWSEWPDDLRIREFPNVRVVSRSF"
     gene            1607..2677
                     /locus_tag="VM910_01425"
     CDS             1607..2677
                     /locus_tag="VM910_01425"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HVI61245.1"
                     /translation="MHCGSFSNLAILLLVATSSGGARAADSDSEHRLSGPAVSENLAV
                     YFVLGNSSDGPVPLTLQEAMADDTVRVYETGNVNELAIENRGAEGVFVQAGDIVKGGQ
                     QDRSLTVSLLLPPHSGRIPIASFCVEPNRWSQRGHEDVHHFSSANAAMPSREAMIAMK
                     APSDAGSAMQRSLASDPSQRPIAETRERQHAVWDYVASINRALSANLSSNSGPMPAPG
                     SLQTALEDDKLKEAQGSYIKLLRPAGESDPDTIGYVFAVNGKLEGGDVYSSNALFRKM
                     WPKLLAASVTEAIANRNSAAVTAPSIETVRAFVSAAERGNPTVAKLTQDVRLETRETE
                     QALFFETAWADGRWVHRNYLAK"
     gene            complement(2726..>3598)
                     /locus_tag="VM910_01430"
     CDS             complement(2726..>3598)
                     /locus_tag="VM910_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVI61246.1"
                     /translation="MATACTSGIDTGFVSLGYAKAAVLGVVQGITELMPISSTAHMRI
                     VPALFGWQDPGSAFSAAMQLAALAAVIGYFWRDVRDILIGSLSALVRRDFSDHSLRFA
                     FGIVIGTIPIGIAGLALAKTLNACGSPLRALWVVGAACIVMALLLGLAELAARHQRSF
                     DRVGLVDCLLVGIAQVGALIPGVSRSGSTLTAALFLGFKREEAARLSFLLGLPAIALA
                     GFKELEELQHAGLDVHGWAVLGVGLLVASLSAFVAIWGLLRILERFSAWPFAVYRALI
                     GVVLLAGVAAGWLG"
BASE COUNT          770 a         1137 c         1119 g          572 t
ORIGIN      
        1 ggccggtctc gaccgcggat cggccgagca cctcttcgag gaagaacggc agcgagacga
       61 aggtcaggtt ttgcgcggtg aacgaacaaa tcgagcccac aacggaaagc gcgaacaccg
      121 gccgcgcaaa gagttcgagc gccagcatcg ggtcggccag catgcgttgc cgccagacga
      181 agagcgcacc cgcgacggcg gcgcacgcca gctcgaacgc aaaagccagc gacaaccggc
      241 gaaggttgtc gatgccgatg atcagcgcgc tgaaggtcac cgcgctcaac aaggcgctga
      301 gccaatcgaa atgatgccgg gagagcgggg ttcgcgggtg acaaaccccc ggttctgggg
      361 tcccgggggc tgtggcggtg ttgtcgatga gcaagcagga gttcagccgc ttgtagctac
      421 atgtaactag gacccctgag gcaaggggaa ggtcacgaaa acacctcaag gtgccggtcg
      481 acttcggtcg ccgcttccta aaacgaacgc gagacaaccc ttacattcgg aaactcccgt
      541 attcgaagat catcaggcca ttcgctccag tcaccaccgg cgtagtcatt taactggagc
      601 cgtgtggtgc cttcgaaggg cttgctgcct aacctctgga cgaagatcgg gatgctggcg
      661 ctcctgcact cagcgacaag ggcacgcgcc cattccaggt gaaacggtcg cgacccggca
      721 cctgcttcac cgccattgat cacccattgc agcagcgatg cgaatctgcc gaggcgtacg
      781 ggcgccaact gcggctcgat actgacaccg tgcaccacgg cagggatggt caacagtttc
      841 ggaacgtcgc gcccaagctc ggcctggtca actgatatga ccagccagac attcggatat
      901 ccgccgcccc aatcgttcgg gagcatctct gccgcattgc cgatgcgctt cgtcaaaagc
      961 agccagtcga ggtttcgcgt ccgacggatc agccgccata gccggtcacg taccgccggc
     1021 tcaacctcgt tgtcaaacac gtcagccatc gacgcgcaga aaacgcggtg acggcgtcgt
     1081 gcggcgcccg caagacaatc ccatttcagc ggctcgcgcc aatacgcatc gccaaaatag
     1141 cggcgcgaag cgcgcggccc ccaatgcacc cgcttgaacc atctggtgtc aagcatcatc
     1201 gcgtaacagc gatcgcagag cgggctgacc tgggtacaac cccaccaggg attaaaggtg
     1261 tggtggcacc actcgatctt ggtgaccgct cccattccgc actgctctga tcaagtcaag
     1321 ccgctcagtc gcgacgctaa acgccatccg cagggccgcg gcaacctgca gcccgataca
     1381 tacggcaagg accgctcgat acctaccggc atgtgggttc gcgatctgga tgtgtttggg
     1441 gctcaacgtc tggtttcata ggtcgtatcg cacagacctg cagtgcgcac gaaaatgtcg
     1501 cggttgtgtc tctccacagc cgctttcggc tgccattcga tgctgtcggc gacagcagaa
     1561 agcggatgcc ctggtcgaac cgaacgtttt tgtgcaggaa ttgcggatgc actgtggatc
     1621 gttctcgaat ctggccatac tcctactcgt cgcaacgagc tcgggcggag cccgggcggc
     1681 ggatagcgac agcgagcacc gcctctccgg gccggcggtg agcgaaaatc tcgctgtcta
     1741 tttcgttctt ggaaattcga gcgacggacc ggtgccgttg acgctccagg aggcgatggc
     1801 ggatgacacg gtgcgggtct acgagaccgg taacgtcaac gagttggcca tcgaaaatcg
     1861 cggcgcagaa ggggtattcg tgcaagccgg cgatatcgtg aagggcggcc aacaggaccg
     1921 ctccttgacg gtcagcctgt tgctgccgcc gcattccggg cgtatcccga tcgcttcatt
     1981 ttgcgtcgag ccgaaccgtt ggtcccagcg cgggcatgag gatgtccatc acttctctag
     2041 cgccaatgct gcgatgccgt cgcgcgaagc gatgatcgcg atgaaggcgc cgtcagacgc
     2101 cggaagcgcg atgcaacgaa gtctggcttc ggatccgtcg cagcgcccga tcgccgaaac
     2161 acgggagcgc cagcacgcgg tatgggatta tgtcgcgagc ataaatcggg cgctgtccgc
     2221 caatctcagc agcaactccg gtccgatgcc tgcgccgggg agccttcaga ctgcgctcga
     2281 agacgacaag ctcaaagagg cacaggggtc ctacatcaaa ctgctgcgcc cggccgggga
     2341 gagcgacccg gacaccatcg gctacgtctt cgccgtgaac ggcaagctcg agggtggtga
     2401 cgtgtattcc tcgaacgcgc tgtttcggaa aatgtggccg aagctccttg cggccagcgt
     2461 taccgaggcg atcgccaacc gcaacagcgc cgctgtcacc gccccttcaa tcgagacggt
     2521 aagggcattc gtcagcgccg cggaacgcgg aaatccgacg gtagcgaaac tcactcagga
     2581 cgttcgcctg gaaacgcgcg agacagagca ggcgctcttt ttcgagaccg cctgggccga
     2641 tggccggtgg gttcatcgca actatctcgc caagtaacgt aatttatgtg gtcctgtacc
     2701 gcgggtagcc gcgttctgtc ggctgtcaac cgagccagcc ggcggcgacg ccggcgagca
     2761 atacaacgcc aatcaaggcc cgatagacgg caaacggcca ggccgagaac cgctccagga
     2821 tgcgcagcag cccccagatc gccacgaacg ccgagaggct cgcgacgagg agcccgacac
     2881 cgagcaccgc ccagccgtgc acatcgaggc ccgcatgttg cagctcctcc agctccttga
     2941 agccggcgag cgcaatcgcc ggcagcccaa gcaggaaaga gagccgggct gcctcctcgc
     3001 gcttgaagcc gagaaagagt gccgccgtca acgtcgagcc cgaacgcgag acaccgggga
     3061 tcaacgctcc aacctgcgcg atcccaacaa gcaagcagtc gacgaggccg acccggtcga
     3121 acgagcgttg atggcgcgcg gccagctccg cgaggccgag cagcagcgcc atgacgatgc
     3181 aggccgcacc caccacccag agggcgcgga gtggagagcc gcaggcgttg agcgtcttgg
     3241 ccagcgcaag tccggcaatg ccgatcggta tcgtgccgat cacgatcccg aaagcaaatc
     3301 tgagcgagtg atcggagaag tctcgccgga caagtgcgga cagcgagccg atgaggatat
     3361 ccctgacgtc tcgccaaaaa tagccgatga cagcggcgag tgccgcgagt tgcatcgcgg
     3421 ccgagaatgc cgaccccgga tcctgccagc cgaagagcgc gggcacgatg cgcatatgcg
     3481 ccgtcgacga gatcggcatc agctcggtga tgccttggac gacgcccagc acggccgctt
     3541 tcgcatagcc gagcgagacg aacccggtgt cgatgccact ggtgcaagcg gtggccat
//
LOCUS       DATLJT010000069         5870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2759924,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000069 DATLJT010000000
VERSION     DATLJT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759924"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM910_01435"
     CDS             <1..315
                     /locus_tag="VM910_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI61247.1"
                     /translation="RQPKAYPVYDDAYRDHMAVIRRDLEQSYPSLHLVGRNGMHKYNN
                     QDHAMMTAMLTARNILASERIYDIWQVNEDAEYHEAGASSAQTALASERLVPLKANTR
                     AA"
     gene            598..1383
                     /locus_tag="VM910_01440"
     CDS             598..1383
                     /locus_tag="VM910_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI61248.1"
                     /translation="MKLSGKVAVITGAARGIGKACAERFLDEGAKVVISDIDAAGLAR
                     TASEFGRPNEVRAVEADVAKRADVDRAVATAIEQFGRIDIMVNNAGVARNQDLLEISE
                     KDFDEIIAINLKGAFFGVQAAAKQMIAQGGGGVIINMSSVNALLAIPSLATYAISKGG
                     MKQLTSVAAVALAPHRIRVVAVGPGTILTDMVASAIFSSEDARRSVLSRTPIGRGGDP
                     GEVAAVVAFLASDDASYITGQTIYPDGGRLVLNYTVPVKEPGK"
     gene            1581..2522
                     /gene="htpX"
                     /locus_tag="VM910_01445"
     CDS             1581..2522
                     /gene="htpX"
                     /locus_tag="VM910_01445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HVI61249.1"
                     /translation="MNYLRTAILLAGLTALFMGVGYLIGGSSGALIALLIAAATNLFA
                     YWNSDRMVLSTYGAHEVDRESAPELVQLVAELAGRAGLPMPRVFVMDNPQPNAFATGR
                     NPQNAAVAVTTGLMHSLSREELAGVIAHELAHVKNHDTLLMTITATIAGAISMLAQFG
                     MFFGGHRNNNSGPGVIATIVMMVLAPLAAMLVQMAISRTREYAADNLGARIAGQPMWL
                     ASALEKIEGAAHQIPNMDAEHNPATAHLFIINPLSGHGVDNLFATHPSTANRIAVLQQ
                     LAAELGTHSPTPWVQTESDVSARSPWNRSPPARGPWG"
     gene            2600..4420
                     /locus_tag="VM910_01450"
     CDS             2600..4420
                     /locus_tag="VM910_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVI61250.1"
                     /translation="MALKIEDYAMIGDCKSAALVGRNGSIDWLCWPRFDSAACFAALL
                     GECENGRWLIAPKEEPIKVSRRYRPGTLVLETEFRTKTGSASLVDFMTPGEGADLVRI
                     LIGRQGRVDFRVEFVVRFDYGATVPWVNRLDDGGLSAIAGPERLVLRTAAALYGEDLK
                     TVGEFTVEAGQSIPFVLSYGPSFQNPPPAIDAFDALERTEAFWRRWSDRCPEIGPWTE
                     AVKRSLITLKALTYAPTGGIVAAPTTSLPERIGGVRNWDYRYCWLRDATFTLLALMYL
                     GYYDEARAWRDWLIRAVAGSPQQVQIVYGVGGERWLPEMTVPWLTGYEKTAPVRIGNA
                     AYDQLQLDVLGEVADALFQAGKVGIGLTDRGRALRPIILEYLAKVWREPDEGIWEVRG
                     GRQHFVHSKVMAWVAFDRAANGFAAEGSTEAAQHWRAVADEIHAEVCKRGFDRDLDSF
                     VQSYGSKRLDASLLLIPLVGFLPATDPRVKGTLRAVEEKLLVADEFVLRYEPENPGDG
                     LPAGEGAFLVCSFWLVDNYIMQGRYAEARKLFDRLLSRRNDVGLLAEEFDPVAGRLLG
                     NFPQAYSHVGLINCALNLTRETCPAERRADARRPPTPATA"
     gene            4605..>5870
                     /locus_tag="VM910_01455"
     CDS             4605..>5870
                     /locus_tag="VM910_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI61251.1"
                     /translation="MQQRGRSGQPAKGRRANRPKARKGSTAAPSIADLQRQVGTLTRE
                     LKEANERQTASAEVLRVINSSPGDLAPVFDAILEKAHRLCGVSQGSLQLYDGEKFRAV
                     AVHGLSEAFADRLRRGFRLGPNSPGQRLLDGARFVQIPDLGEIDDPMSRAAFELAGVR
                     TILFIPLRKDGTFFGRISASRPEVKPFTEMEIVLLESFAAQAVIAIENARLFEEVQAK
                     TCDLTEALTYQTGSANILKVIASSPTDVGPVLTAIVESACVICEAENAHVTLKDGDEL
                     VFQAQHGSAPVVWKRQPINRRWVTGRAAVDRRPIHVHDLLSSEGDEFPDGQELARRDG
                     ARTMLAVPLLREGECVGVIVLRRMEVRPFSEKQIELLQTFADQAVIAIGNVRLFGEVQ
                     AKTRDLEESLQQQTATSEVLQVISSSPGDL"
BASE COUNT         1146 a         1763 c         1850 g         1111 t
ORIGIN      
        1 cgtcagccca aagcctaccc cgtctatgac gacgcctatc gcgaccatat ggcggtgatc
       61 cggcgcgacc tggagcaaag ctatccaagc ttgcaccttg tcggtcgaaa cggaatgcac
      121 aaatacaaca atcaggatca cgccatgatg accgccatgt tgacggcgcg aaacatcctg
      181 gccagcgaac gcatttacga catttggcaa gtcaacgagg acgcagaata tcacgaagca
      241 ggagcatcga gcgcgcagac agcacttgca agtgaacgcc tggtgccgct caaggccaac
      301 acccgcgctg cttgaagcct ctacgaggtg atcgagtgag gcgcgtattc gcttcgagtc
      361 cctctagtcg aagggcttgg ttgggagcgc cacttctggc gatgccgggc agaaacaggc
      421 agaaaatttg gcggtgctca atcggaacgg gctaaaccgc tcgccactcg gctccttacc
      481 gcgcgagggg cttgcgctca gctctctttc tcagcaaccc gattcgtgct catcttgggg
      541 ggcggcctga gccgatgtcc ggcttgcgag ctgcatgagc ttccccggga gtgatttatg
      601 aaactatccg gcaaggtcgc ggtcattacg ggtgcggcgc gaggcattgg gaaagcgtgc
      661 gctgaacgat ttctcgacga gggcgccaag gtcgtcattt cagacatcga tgccgccggt
      721 ctggcaagga cggcatccga attcggccga cccaatgagg tgcgagcggt cgaggcggat
      781 gttgcaaaac gcgccgatgt cgatcgtgcc gtggcgaccg caatagagca atttggccgg
      841 atcgacatca tggtcaacaa tgccggcgtc gcacggaatc aggatctgct cgagatttcc
      901 gaaaaggatt tcgacgagat cattgcgatc aacctgaagg gcgcattctt cggtgtgcag
      961 gccgcagcga aacagatgat cgcgcagggc ggtggcgggg tcatcatcaa catgtcgtcg
     1021 gtcaatgcgc tgctggccat tccctcgctt gcgacctacg ccatctccaa gggcggcatg
     1081 aagcaactca cctcggttgc ggcggtggcg ctcgccccac acaggattcg ggtcgtggcg
     1141 gtcgggccgg gaacgatttt gacggacatg gtagcatcgg cgatcttctc gtccgaagat
     1201 gcgcgccgca gcgtgctgtc gcgaacgccg atcggtcgcg gcggcgatcc aggtgaagtg
     1261 gccgccgtcg tggccttcct ggcaagcgat gatgcttctt acatcaccgg ccagaccatc
     1321 tatccggatg gcggccgact ggttcttaac tacacggtgc cggtcaaaga gccgggtaaa
     1381 tgaaagatca aatggggcgc gcctcgatcc gcggctggtc atgggacgcg ccttggtgac
     1441 ccgagcaggg cggacggcat gccggcccgg caatctgttt cggcgtaaag acgcaacgcc
     1501 cagtcttgca gcgccgaaag gcagatacca atttgagttg gagttgccgc gccggacggc
     1561 cgcgctcgag ggaggtggca atgaattacc tgcgaactgc aattctgctg gcaggcctca
     1621 cagccctttt catgggcgtc ggttatctga tcggtggcag cagcggtgca ttgatcgcgt
     1681 tactgatcgc ggccgcaacc aacctcttcg cctattggaa ctcggaccgc atggtgctgt
     1741 cgacctacgg cgcccacgag gtcgatcgcg aatccgcgcc cgaactcgtc caactcgtgg
     1801 ccgaactcgc tggccgcgcc ggcctgccga tgccgcgcgt gttcgtcatg gacaatccgc
     1861 agccgaatgc ctttgcaacc gggcgcaacc cgcaaaatgc ggcggtggcc gtgaccaccg
     1921 gattgatgca ttcccttagc cgggaagaac tcgccggtgt gatcgcccac gagctcgcgc
     1981 atgtcaaaaa tcacgacacg ctgctgatga ccatcaccgc aaccatcgcc ggcgcgatct
     2041 cgatgctggc gcagttcggc atgttcttcg gtggtcatcg caataacaac agtgggccgg
     2101 gcgtgatcgc gacgatcgtc atgatggttc tggcaccgct cgccgccatg ctggtgcaga
     2161 tggcgatcag tcgcacacgc gaatacgccg ccgacaatct cggcgcgcgc atcgccggac
     2221 agccgatgtg gcttgcttcc gccctggaaa agattgaagg cgctgcccat cagatcccga
     2281 acatggatgc cgagcacaat ccggcgacgg cgcacttgtt catcattaat ccgttgtcgg
     2341 gtcatggggt cgacaacctc tttgcgacgc atccgtcgac cgcaaaccgc atcgccgtgt
     2401 tacagcagct cgcagccgag ctcggcacgc acagcccaac accttgggtt caaaccgagt
     2461 ccgatgtttc tgctcgcagt ccatggaatc gttcaccgcc tgctcgtggg ccgtggggat
     2521 gagggcgccc gcgcgcaata caaacttgta ctgaaaacag gtagtatggg tatcgcgccc
     2581 gaccaggcaa aggcacgcga tggcgcttaa aatcgaagat tatgcgatga tcggcgactg
     2641 caagagcgca gcgctggttg gccgaaacgg gtcgatcgat tggctgtgct ggccgagatt
     2701 cgactcggcc gcgtgctttg cagccctgct cggagaatgc gaaaacgggc ggtggctcat
     2761 tgcaccaaag gaagagccga tcaaggtgtc gcgccgctac cgcccgggta ctctggtcct
     2821 cgaaacggaa ttccgaacga aaaccggcag cgcgagccta gtcgatttca tgacgccggg
     2881 agagggagcc gatcttgtgc gcatccttat cggccggcag gggcgagtgg attttcgcgt
     2941 ggaatttgtg gtgcgtttcg actacggggc gacggtgcca tgggtcaatc ggctcgatga
     3001 cggcggcctc agcgcgattg ccggtccgga gcgcctggtg ctgcgtaccg cggcggcact
     3061 ctacggcgaa gatttgaaga cggtcggtga gttcacggtc gaggccggcc agtcaatccc
     3121 gtttgtcctc tcctacggac catcgtttca aaatcccccg ccagcaatcg atgccttcga
     3181 cgccctggag cgaacggaag cattttggcg gcgatggagc gatcggtgtc ccgagatcgg
     3241 tccctggaca gaggcggtca aacgttcgct gattacattg aaggctttga cctacgctcc
     3301 gacgggtggg attgtcgccg ctcccaccac gtcgctgccc gagcgtatcg gcggcgtccg
     3361 aaattgggat tatcgttatt gctggctacg cgacgcgacc ttcacgctgc tggccctgat
     3421 gtacctcgga tactatgacg aagcgcgtgc atggcgagac tggctgattc gcgccgtcgc
     3481 cggcagcccg cagcaggtgc agatcgttta cggagtaggt ggcgagcgtt ggctgccgga
     3541 gatgaccgtt ccgtggctca ccggctatga aaaaaccgcg ccggtgcgca tcggaaacgc
     3601 cgcctacgat caacttcagc tggacgttct cggcgaagtc gccgacgccc tgtttcaggc
     3661 cggcaaggtg ggaataggtc tgacagatcg tggccgcgcc ctgcggccca tcatattgga
     3721 atatctcgca aaggtttggc gagagccgga tgaaggcatc tgggaggtgc gcggcggacg
     3781 acagcacttc gtccattcga aggtcatggc ctgggtcgcg ttcgatcgtg ccgcaaatgg
     3841 cttcgccgcc gaaggatcaa cggaggcggc ccaacattgg cgagcggtcg ccgacgaaat
     3901 ccacgccgaa gtgtgtaagc gcggcttcga ccgcgatctc gacagtttcg tgcaaagcta
     3961 cggctcgaaa cggctcgatg ccagcctcct gctgatcccg ctcgtcggtt ttctgcccgc
     4021 caccgatccg cgggtgaagg gcactcttcg tgcggtcgag gaaaaactat tggtcgcgga
     4081 cgaattcgtt ctgcggtacg aacccgaaaa tcccggagat ggcctgcctg ccggagaagg
     4141 ggcttttttg gtgtgcagtt tctggcttgt cgacaactac attatgcaag gacgttatgc
     4201 agaggcgcgg aagctgttcg atcgtttgct gtcgcgccgc aacgacgtcg gtctgcttgc
     4261 cgaagagttc gatccggttg ccggccgctt gctcggaaat tttccgcagg cctacagtca
     4321 tgtaggtttg atcaattgcg ctttaaatct cacccgtgag acatgccctg ccgagcggcg
     4381 cgcagatgcg cggcgtccgc cgacgcccgc aactgcttaa gccgaggcgg cgctggctga
     4441 cgggaagatt cgggcagctc ctccgggttt gacgggcgcg gctgaactcg ggctgaaggg
     4501 ccgctggtcg agccttcgcc cctgagtacg gactttccgg catggcgtaa aaccgaagat
     4561 atgcataaga ttgagtccca tcgtggccca cccgagggag cgaaatgcag cagcgtggtc
     4621 gaagcgggca gccggccaag ggtcggcgcg cgaataggcc caaggctcgc aaaggatcga
     4681 ccgcagcccc atctatcgcc gacctgcaaa ggcaggtagg cactcttacc cgcgagctga
     4741 aagaggccaa cgagcggcag actgcgtccg ccgaggttct gcgggtcatc aattcatcgc
     4801 ccggcgatct cgcgccggta ttcgatgcga tcctggaaaa agcgcatcgt ctttgcgggg
     4861 tcagccaagg gagcctgcag ctttatgacg gcgagaagtt ccgcgccgtc gcggtgcacg
     4921 ggctatccga ggcgttcgcc gatcggctgc gccggggctt ccgcctcggc cccaattcgc
     4981 cgggccagcg tttgctggac ggggcgcgct tcgttcagat ccccgacctc ggcgagatcg
     5041 acgatccgat gtcacgtgcc gccttcgaac tggccggcgt ccgcaccatc ctgttcattc
     5101 ccctgcgcaa ggacggcaca ttcttcggcc ggatctccgc ctctcgaccg gaggtcaagc
     5161 ccttcaccga gatggagatc gtccttctcg aaagcttcgc cgcgcaggcg gtcattgcga
     5221 tcgagaatgc gcggctgttc gaggaggtgc aggccaagac gtgcgatctg actgaggccc
     5281 tcacctacca gaccggcagc gccaatatcc tcaaggtgat cgcctcgtcg ccgaccgatg
     5341 tcggaccggt tctaacggcg atcgtagaga gcgcctgcgt aatttgcgag gcggaaaacg
     5401 cccacgtcac cttgaaggac ggcgatgagc ttgtattcca ggcgcagcac gggtcggccc
     5461 cagtcgtttg gaaacgacaa ccaatcaacc gccgctgggt cacggggcgt gcggcagtcg
     5521 atcgcagacc cattcatgtg catgacctcc tctcgtctga aggcgacgag tttcccgacg
     5581 gacaggaact cgcacgccgg gacggcgcgc gaactatgct ggccgtgcct ctgctgcgcg
     5641 agggcgaatg cgtcggcgtg atcgtgcttc gccggatgga ggtacgtcca ttcagcgaga
     5701 agcagattga actgctgcag accttcgccg atcaggccgt gatcgcgatc ggcaacgtcc
     5761 gcctgttcgg cgaggtgcag gccaagacgc gcgatctcga ggaatccctc caacaacaga
     5821 ccgcaacgtc cgaagtgctg caggtcatca gttcctcgcc cggcgatctg
//
LOCUS       DATLJT010000070         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2070847,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000070 DATLJT010000000
VERSION     DATLJT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070847"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VM910_01460"
     CDS             <1..111
                     /locus_tag="VM910_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI61252.1"
                     /translation="RCANVCFGGVKRNRLFMAASQSIYSLYVETQGALGG"
     gene            168..983
                     /locus_tag="VM910_01465"
     CDS             168..983
                     /locus_tag="VM910_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI61253.1"
                     /translation="MSGQAASFVGSIPQHYDRGMGPVIFADFADDIARRVAAFVPMRV
                     LEIAAGTGIVTRRLRDTLPAAAELTATDLNSPMLDVARIKFAPDEQVEYRPADATSLP
                     FPNGAFDAVVCQFGVMFFPDKERSYREVHRVLSSGGHYVFNVWDAHRHNPFGRLVHET
                     IGGFFAADPPQFYQVPYGYHRIDPVKDSLTNAGFTDLRIAVIRLECQIPDARAFALGL
                     IYGNPVIIDQIQTRGGVDPDRIVDALTEALRREFGPDPGCLPLQAIVFEARRD"
     gene            1156..1293
                     /locus_tag="VM910_01470"
     CDS             1156..1293
                     /locus_tag="VM910_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61254.1"
                     /translation="MQIIVVLVIIWILFLVLSWLLHAVIWLLVVAIIVSLAAVAAKYV
                     L"
     gene            1733..2701
                     /locus_tag="VM910_01475"
     CDS             1733..2701
                     /locus_tag="VM910_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61255.1"
                     /translation="MKPQSYTYSPHYPVLQYNATQANFYGGNFYDLRATGYKLMSPDS
                     KQAQGPVVDTQEMGFPDFACVVFRLSKAVYRPLFEGIWRASFAIRWPFNTERICSTAG
                     GSAMLGGKPTPVNLTPVERGIANATFDAYAHSLTAYKASPDVSAFSSKFEASLAGNTT
                     LTVDEKAGYDLFGGKANCNSCHLDGRGVARTPDLSDNSNATDAGPLFTDTTSANLGLP
                     KNPAVPFYYETTRDSLGFTPNPVGFDFTDLGVGRFLRGGSDTNPNANWRQYADKFDGF
                     MQVATLRNVDMRPCPAFPKSYMHMVIRRASRRSSISITLATRSRSR"
     gene            2698..2919
                     /locus_tag="VM910_01480"
     CDS             2698..2919
                     /locus_tag="VM910_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61256.1"
                     /translation="MTAGNCPAGTVEKVSCWPMPEVPGTKDMTFINLGLTDREEDHIV
                     AVLQTLTDGFTTPYPDLNTYTGIVCQQSK"
     gene            3514..4032
                     /locus_tag="VM910_01485"
     CDS             3514..4032
                     /locus_tag="VM910_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61257.1"
                     /translation="MPSIGDTTARVQPGKPAPALKLNFLSHGTLESRDLAFSRRFYEE
                     FLGLEVVQTSPISLLIRLGGPNTIAVVYSKKLGGMNLLNHNGLDVRTQEEVDQAHRIV
                     CAQAEEWHLKKISKPHLQHGTYSFFFWDGDDNCWEILTNPEGGYSWLFEQGDQQGKGH
                     LDRHFKRPGVAT"
BASE COUNT          799 a         1274 c         1168 g          830 t
ORIGIN      
        1 cgctgtgcca acgtctgctt cggcggggtc aaacgcaacc gcctctttat ggcagcgagt
       61 cagtcgatct attcgctcta cgtcgaaacc cagggcgcgc tgggtggctg agcagcctct
      121 ggcggcgttg caagcgacac cggaggagca ggaaagggaa cgacgcattg agcggccagg
      181 cagcaagctt tgtcggcagc attccgcaac attacgaccg cgggatgggg cccgttattt
      241 tcgccgattt cgccgacgac attgcgcgtc gggtagcggc gttcgttccg atgcgcgtat
      301 tggagatcgc cgccgggacc ggcatcgtaa cccgtcgtct aagggatacg ctcccggccg
      361 ccgccgagct gaccgcaacc gacctgaact caccgatgct cgatgtggca cgcataaaat
      421 ttgcgcccga cgagcaggtc gagtaccggc ccgccgacgc cacgtcgctt ccgttcccca
      481 atggcgcctt cgatgcggtc gtctgccagt tcggcgtcat gttcttcccg gacaaggaga
      541 ggtcctaccg cgaggttcac cgcgtcctgt cgtccggcgg ccattacgta ttcaacgtct
      601 gggacgccca ccgtcataac ccgttcgggc ggctcgtgca cgaaacgatc ggtggcttct
      661 ttgccgccga tcctccacag ttctaccagg tgccgtacgg atatcaccgg atcgacccgg
      721 tcaaggactc gctaaccaat gccgggttca ccgacctcag gatcgccgtt atccggctcg
      781 aatgccagat tccggacgcc cgcgcatttg cgcttggcct gatctatggc aacccggtga
      841 tcatcgacca aatccagacg cgcggtggag tcgatcctga ccggattgtc gatgccctga
      901 ccgaggcgct gcgccgcgaa ttcggtccgg atccggggtg cctcccgctc caggcgatcg
      961 tattcgaggc aaggcgagac tgatcgggcc tgcccaggac ccggccgatt gcgcgccgcg
     1021 aaggtgcaag ttctcggcca gtccctccct gctgacaacc attgaacgcg tctttggaaa
     1081 acgacggcag tctcagcacg ggtgtcgcag ctgtgatgcc ccgagctgac ttagcagagg
     1141 aggtaacctc gcgtcatgca gatcattgtc gttctcgtga taatctggat cttgttccta
     1201 gtgctctcct ggttgctgca cgcggtcata tggttgctcg tcgtcgccat catcgtgagc
     1261 ctggcggctg tcgccgcgaa atacgtgctg taaatcaccc gttagcggta aacgtgcccg
     1321 gaacccctgg gtctgaagac gtgcagtccg gcccccgagc caaatatttt cccgaggatc
     1381 tcacatcgga accggaggcc gcgccaatca cgcggcgctg caataatcca gcaagggcgt
     1441 cgtgggtcgc ccggtgaggt gcgatgacaa agcgcgctcc ggaaaggaag ccctcgcgga
     1501 atggcatgcc ttggggggcc cctcaccccc tccggcgatc cgcttgtgct cgcaggaagc
     1561 ggaatgcgcg ggcttcaggt tctcggcaag ctcgaacact ttgatgagca gctctcggtc
     1621 aacaagaatg aggcgtgcgc cttttgccac atgccgtata gcggatttac ccgccctatc
     1681 ccctcactga atcgcaccac agtggcctat ccgggatcgt ttcactgccg ctttgaagcc
     1741 gcaatcctac acctattcac cgcattatcc ggtgcttcag tacaacgcca ctcaggccaa
     1801 tttctacggc ggcaacttct atgacctgcg cgcgaccggc tacaagctga tgagccccga
     1861 ttcgaagcag gcgcaagggc cggtcgtcga tacgcaagag atggggtttc cggatttcgc
     1921 ttgcgtggtg ttccggcttt ccaaggcggt ttacaggcct ttgttcgaag gtatttggcg
     1981 cgcgtctttt gcgatccgct ggccattcaa taccgagagg atctgtagca ccgcaggcgg
     2041 ctcggccatg cttggcggca aacctactcc cgtcaacttg acgcccgttg agagaggtat
     2101 cgcaaacgct acgtttgacg catacgcgca ttccctcact gcctacaagg cctcgcctga
     2161 cgtcagcgcg ttctcctcga aattcgaagc atccttggcc ggcaacacca cactgactgt
     2221 ggacgaaaaa gcgggctacg atttattcgg cggcaaggcg aactgcaatt cctgccatct
     2281 cgacggaaga ggggtcgccc gcacgccaga tctgagcgac aacagcaacg ctacagatgc
     2341 cgggcccttg ttcaccgata cgacgtcggc caatctcggc cttcccaaga acccggcggt
     2401 tccgttttat tatgagacca cgcgagattc tttgggtttt accccgaatc cggtgggttt
     2461 cgacttcacc gatttgggag tggggcgctt tctaaggggc ggatcggaca ccaatccaaa
     2521 cgcaaattgg cggcaatacg ctgacaagtt cgacggtttc atgcaggtag ccactctccg
     2581 caatgtggat atgcggccct gtcctgcctt cccgaagagt tacatgcata tggttatccg
     2641 aagagcctca aggaggtcgt ccatttctat aacactcgcg acgcgttcgc gaagccggtg
     2701 accgcaggga actgcccggc gggaaccgtc gagaaggtga gttgctggcc catgccggag
     2761 gtgccgggaa ccaaagacat gacgttcatt aatctcggct tgaccgatcg ggaagaggac
     2821 catatcgtcg cagtcctgca aaccttgacc gacggcttta caacgccgta ccccgacctc
     2881 aacacctaca ctggtattgt ctgccaacag tcaaaatgag gcttttgctt gtcgcggttt
     2941 ctgcgccgtg cagccggcaa gctagcccca ttgggacgat tgccatgcgg gcgcaatcaa
     3001 ccccgctttg cgtattacgg tccagtaccg accgtaatat tactgcggcg gctactgaat
     3061 gctccgatta gagagcggcg cccctggcaa ttcgccgctc gcccgaatgc agcgagttaa
     3121 cgttgtaaag ccacgggcct ggcagccgcg gatcgaggat gtcgcggatg ccgtcgcccg
     3181 cgcgaattga aagcgagacc agtgctcgta atctcgaagg cgggtcaccc ttccgctgaa
     3241 cgggcggcgc cgcgcgattg catacccgca attttacata tctgattcac atgacctaat
     3301 ttttcccgtg tatgccgtga tacatcttcg ccccgcttag cactcgcgct cggtgcgctg
     3361 tcgttcgctc aacccgcggt ctgcgtctcg acgagcgcgg caatcgcggt ctgcctttcg
     3421 ccggaggggc gctcagcggt tgaggctgtt cccgcgattg tgctcagccc caggctggct
     3481 atgatcggct caggcaaatg catggaggct ctgatgccat caattggaga cacgaccgca
     3541 cgggtgcagc ccggcaaacc ggcgccagca ctcaagctca actttctgtc gcacggcacg
     3601 ctggagagcc gcgacctcgc tttctcacgc cgcttctacg aagagtttct cggtctcgag
     3661 gtcgtccaga cgagcccgat ctctcttctc attcgattgg gcggccccaa caccatcgcc
     3721 gtcgtctaca gcaagaaact cggcggaatg aaccttctga accacaacgg actcgatgtc
     3781 cgcacccagg aagaggtgga tcaggcgcac cgcatcgtct gcgcccaggc cgaagagtgg
     3841 cacctcaaga aaatctccaa accgcatctt cagcacggca cctacagctt cttcttttgg
     3901 gatggcgacg ataattgctg ggaaatcctg accaacccgg aaggcggcta tagttggttg
     3961 ttcgaacagg gcgaccagca gggcaaaggg catctcgacc gccacttcaa gcgacccggc
     4021 gtcgccacct gagcctgttg cggagccgcg catgcggcgt cgggatctcc t
//
LOCUS       DATLJT010000071         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4368140,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000071 DATLJT010000000
VERSION     DATLJT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368140"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VM910_01490"
     CDS             <1..294
                     /locus_tag="VM910_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336158.1"
                     /note="type IV secretion VirD4 coupling protein family;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HVI61258.1"
                     /translation="RGADDWSHLPVPASVEDAAGPIAGSFSDQDSENSGIRREPTLPD
                     HEAIVAEEPKPGTEFTFCDDEPDDDAIRAGALRQRFGTVARQAALDPDDGIPL"
     gene            304..759
                     /locus_tag="VM910_01495"
     CDS             304..759
                     /locus_tag="VM910_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVI61259.1"
                     /translation="MKERLSVYLDPHVMRSLIDYADRRGKSKSLVAEAAIASFLSPDA
                     AERQEATLARRLDRLVRQTERLERDVGIAVETLALFVRFWLMATPALPEQAQAAARAK
                     GAERYHAFIEALGRRIAKGPTLLREVSVDVAAASDSDASAKPVGDTRES"
     gene            902..1894
                     /gene="trbB"
                     /locus_tag="VM910_01500"
     CDS             902..1894
                     /gene="trbB"
                     /locus_tag="VM910_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HVI61260.1"
                     /translation="MVGAFTFRTEAISRGARMLRTALGPAIAAWLEDPGVVEVMLNPD
                     GRLWVDRLTEGLIDTGERLAAADGERIVRLVAHHVGAEVHSAAPRVSAELPETGERFE
                     GLIPPVVAAPAFAIRKPAVAVFTLDDYVAAGIMSADEAGVLRRAVEKRRNIFVAGGTS
                     TGKTTLVNALLAEVAKTTDRVVLIEDTRELQCKSPNLVALRTKDGVASLSDLVRSSLR
                     LRPDRIPIGEVRGAEALDLLKAWGTGHPGGIGTIHAGSALGALRRLEQLIQEAVVTVP
                     RALIVETIDLIAVLAGRGPERRLAELAAIEALGPNGDYVLVSTASDFEAEGEKA"
     gene            1891..2223
                     /locus_tag="VM910_01505"
     CDS             1891..2223
                     /locus_tag="VM910_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HVI61261.1"
                     /translation="MTRSAAHTHHRIAIAFTVSFLAWTTITPAHAAGSNMPWEQPLQQ
                     ILQSIEGPVAKIMAVIIIIVTGLTLAFGDTSGGFRRLIQIVFGLSIAFAASSFFLSFF
                     SFGGGALV"
     gene            2223..>2368
                     /locus_tag="VM910_01510"
     CDS             2223..>2368
                     /locus_tag="VM910_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HVI61262.1"
                     /translation="MSEIHDPIPGFEAPVHRALTEPILLGGAPRAVAITNGTLAAAVG
                     LGLRL"
BASE COUNT          396 a          763 c          755 g          454 t
ORIGIN      
        1 aggggagctg acgactggag ccatctcccg gtcccagcgt cagtcgagga tgcggctggg
       61 ccgatcgccg gctctttctc cgatcaggat tccgaaaaca gcggcatccg gcgtgagccg
      121 acgctgcctg accacgaagc gatcgtggcc gaggagccga agcccggcac agagtttact
      181 ttctgcgatg atgagcctga cgatgacgcc atccgtgctg gagcactgcg gcagcgcttt
      241 gggacagtcg cgcgacaggc cgcgctcgat ccggatgatg gcatcccgct ctgaggcccg
      301 cccatgaaag aacgtctgtc ggtctatctc gatccgcacg tcatgcgctc gctcatcgac
      361 tatgccgatc gccgaggcaa atcgaagtcg cttgttgccg aagcagcgat tgcctcgttt
      421 ttgtcacccg acgcagccga gcggcaggaa gcaaccttgg cgcggaggct cgaccggttg
      481 gtccggcaga cggagcggct tgagcgcgat gtcgggatcg cggtggagac cttggcgctc
      541 ttcgttcgtt tctggctcat ggcgacgccg gcgttgccgg agcaagccca ggctgctgcg
      601 cgcgccaaag gcgccgaacg ataccatgcc ttcatcgagg ctctcggtcg gcgaatcgcc
      661 aaaggtccaa cgctgctgcg tgaagtgtcc gtggatgtag cagcggcaag tgactcggac
      721 gcaagcgcaa agccggtcgg cgacacccga gaatcgtaac cgccgcgagc gcactccgcc
      781 attcacctgt ctttctacgc cagcgcgcta cgactcgtcg ttcgttgttg cacgcagcca
      841 cgcttggccc tttctaatca tcctcgacat tgggttgcgt gggtcgccgt gggggcgggg
      901 gatggtcggg gcatttacat tccggacgga agcgatctcg cgcggtgcgc ggatgctgcg
      961 cactgcactc gggccggcca tcgcagcatg gcttgaagat cctggcgttg tcgaggtgat
     1021 gctcaatccc gatggccgtc tatgggtcga tcgtctgaca gaagggctga tcgataccgg
     1081 cgagcggctc gcagctgccg atggcgaacg catcgtccgc ctcgtcgccc atcatgttgg
     1141 tgcggaagtg cattcggcag ctccacgcgt ctctgcggag ctgccggaaa ccggtgagcg
     1201 gttcgaagga ttgatacctc cggtcgttgc cgcaccggcc tttgccatcc gcaaacctgc
     1261 cgtcgcggtc ttcaccctcg acgattatgt cgctgccgga atcatgtcag ccgatgaggc
     1321 cggcgtgctg cgccgtgccg tcgaaaaacg acgcaatatc tttgttgccg gtgggacctc
     1381 gaccggcaag accacgctcg tcaatgcgct gctcgccgaa gttgccaaga cgaccgatcg
     1441 cgtcgttctg attgaagata cgcgcgagct ccagtgcaaa tcgccgaacc tggtggcgtt
     1501 gcgaaccaag gacggcgtgg cctcgctgtc cgatcttgta cgctcctcgc ttcggctgcg
     1561 gccagatcgc atcccgatcg gcgaggtgcg cggcgcagaa gcgctcgacc tcttgaaggc
     1621 gtggggtacc ggacatcccg gcgggatcgg caccattcat gccgggtcgg ctctcggcgc
     1681 gctgcgccgc ctggagcagc tcatccagga agcagtcgtc accgtaccgc gcgccctcat
     1741 cgtggagacg atcgacctga ttgccgtgct cgccggtcgc gggcccgagc gacgacttgc
     1801 cgagctcgcc gcgatcgaag cactcgggcc gaacggcgat tatgtcctcg tatccacagc
     1861 atccgacttt gaagccgagg gagagaaggc atgacccggt cggccgcaca cacccatcac
     1921 cgcatcgcga tcgcgttcac cgtctcgttt ctcgcatgga cgacgatcac accggctcac
     1981 gccgctggtt cgaacatgcc ctgggagcaa ccgctgcagc agatcctgca gtcgatcgaa
     2041 ggtccagtgg cgaagatcat ggcggtgatc atcatcatcg tcaccggcct gacgctcgcc
     2101 ttcggcgaca cgtcgggcgg cttccggcgc ctgatccaga tcgtgttcgg cctctcgatc
     2161 gcgtttgcag caagttcgtt cttcctgtcg ttcttctcgt tcggcggtgg agctctggtc
     2221 tgatgagcga gatccacgat ccgatccccg ggttcgaagc gccggtccat cgcgccttga
     2281 ccgagccgat cctgcttggc ggtgcgcccc gcgcggtcgc gatcaccaac ggcacgctcg
     2341 cggctgctgt cggattgggg ctgcgcct
//
LOCUS       DATLJT010000072         2946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381463,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000072 DATLJT010000000
VERSION     DATLJT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381463"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..407)
                     /locus_tag="VM910_01515"
     CDS             complement(12..407)
                     /locus_tag="VM910_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61263.1"
                     /translation="MTRLILALLVLAISILPLYAQSTSLHTDRSGYTTGTDGNRTVNT
                     YTDRYGNTTGWVGGKYISTYSDGYGGTTGAIGNKHISTYEDGYGSTTGVIGRDRINLY
                     TDPSGTTTGTIGRRRLNCYTDRFQTTTCN"
     gene            complement(599..769)
                     /locus_tag="VM910_01520"
     CDS             complement(599..769)
                     /locus_tag="VM910_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61264.1"
                     /translation="MMPADLKRFHKYMLEVEHIDHISDEMRAVVESEWPELAHKLLPE
                     GRITSRVSTLER"
     gene            complement(1620..1847)
                     /locus_tag="VM910_01525"
     CDS             complement(1620..1847)
                     /locus_tag="VM910_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61265.1"
                     /translation="MNEETKAARESSEASWKSLEASLAAVEREAKMMAARIRRNVILE
                     LLKQARDPDERRVIEEVSSRMLRQDKAEGRP"
     gene            complement(1949..2374)
                     /locus_tag="VM910_01530"
     CDS             complement(1949..2374)
                     /locus_tag="VM910_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI61266.1"
                     /translation="MAVRLQLPRKKAPSPTDQHVGARVRMRRKELAMSQEQLAEALGI
                     TYQQVQKCEKGANRIGASRLQQISHILQVPVAFFFEGAPNASAPHGSNGSALSMAQID
                     DFVSDSDGLRLIGAFMRIDNAALRRRIVMLVQEIAGDDD"
     gene            2744..>2946
                     /locus_tag="VM910_01535"
     CDS             2744..>2946
                     /locus_tag="VM910_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM domain-containing protein"
                     /protein_id="HVI61267.1"
                     /translation="MQQIADWLEKLGLSEYTARFVENGIDISVLRYLTDQDLKDIGVL
                     LGHRRKMLAAISELTGPIPVTSEA"
BASE COUNT          535 a          896 c          884 g          631 t
ORIGIN      
        1 gcgatcagtc cctaattgca tgtggtggtt tgaaaacggt cagtgtaaca gttcagacgc
       61 cgtcggccga ttgtccctgt ggtggtgccg gaagggtcgg tgtagagatt tattcgatct
      121 ctgccgatga cacccgtggt gctgccgtag ccatcttcgt atgtggatat gtgcttgttg
      181 ccgatcgcac ccgtcgtccc gccgtagccg tcgctataag tgctgatgta cttaccgccg
      241 acccagcctg tcgtattgcc gtaccgatca gtgtaggtgt ttacggtgcg attgccgtca
      301 gtgcctgtcg tataaccaga tcgatccgtg tgaaggctag tgctttgggc gtacaacgga
      361 agaatggaaa tcgcgagcac cagcaacgca agaatgaggc gcgtcataga acacctcctt
      421 tgctgttagt gcgctgcgat gacgctggac gatgatagcg caatcctcat gctgcctcca
      481 tcgccgcccg aatcccggct cctgggcggt cactggcgcg cctcgatctc ttgcgccgtg
      541 tattcgccgc gctcaggctg gccacccttc catcgggtcc cgctgcggac cgtggtgatc
      601 aacgttcgag tgttgacacg cgtgacgtga tgcggccctc cggtagcaac ttgtgcgcca
      661 actcgggcca ctcgctttcg accacggcgc gcatttcatc cgagatgtgg tcgatgtgct
      721 cgacctccag catgtatttg tggaatcgct ttaggtccgc cggcatcatg accttgccgc
      781 cgactatgcg gggcttatta gacgcggcgt tcttttctca tcagcgccta ctgtcgtcga
      841 tcacgcgggc accctgacac ggggtaacga ccgcttccgg ccatcgtttt atggccgccc
      901 gatgtcacca cggcagcgac gaacaaatgt ttggcgcaaa gtaacaagtc ccgcatggag
      961 ggctgaagcg actaagaagc ggacgacgac gttcaaccgc ggcatcgtca ggaaggtcaa
     1021 gcgcgggctc taccgatggc acggatgacc gagcacgacg tgatcggatg gttcaatgcg
     1081 gcgccgatgg gctcgcggca acagcggatg ctatgggccg cgcacaagat cgcgcgcctg
     1141 actgcggcca ccaagggcag cgtttatcag atgctagaac gcaggttgga acgtgccgga
     1201 cccctccctc caggccctca gttcaacggc agcgccgggc cgcgctcggc cgccttggct
     1261 ctctggcgat ctcgcgctcg cggtagagcg cggcgagttc ggcctcggca tgctgccggg
     1321 cgcggaccgt cgcctgtagc tcgcgcagag cggtgctcgc acgggcttct acagcccgat
     1381 taagtaccgc gattctttgc actgaaaagg ggcccccgtt ttctctccgg cctccctcgc
     1441 cccgcgcgtc ccctccggcg gagatacgcc caaagaggca acgcgccgaa aatgaaagac
     1501 ccgttcgaga aaagaccgcg aaaagatgaa cgcggccctt tcggttgcgc ttagggcgtg
     1561 gtcggcggca aaggatgggg atttggtcga tctggtgcgc cgcctcaatt tgggttgcgt
     1621 catggcctac cctccgcttt gtcctgccgc agcattcggc tgctcacttc ctcgattacc
     1681 ctccgctcgt ccgggtcgcg agcttgttta agcaactcga gaatgacgtt gcgccttatc
     1741 ctcgctgcca tcatcttggc ctctcgctca acggcggcca gggatgcctc tagactcttc
     1801 cagctggcct cggagctttc ccgagcggct ttcgtttcct cgttcatggc tcggctcctc
     1861 cgatcaggtc ggtagtccgc agcagatcgt tttcgatccg ctccagattc tcagccgcga
     1921 gcgtcgcagc ggccgagatc gcggcatcct agtcgtcgtc accggcaatt tcttgcacga
     1981 gcatcacgat cctgcgccgc agggcggcat tatcaattcg catgaacgct ccgatcagcc
     2041 ttaagccgtc tgaatcggag acgaagtcat caatctgggc catcgataac gcgctcccat
     2101 tggaaccgtg tggcgctgat gcgttcggcg cgccctcgaa gaagaacgct actggcacct
     2161 gaaggatatg tgagatttgc tgcaaccggc tggccccgat gcggttggcg cccttctcgc
     2221 atttctgcac ttgctggtag gtgatgccga gcgcctcggc cagttgctcc tggctcattg
     2281 ccagctcctt gcggcgcatc cgcacccggg ccccgacgtg ctggtcggtg gggctcgggg
     2341 ccttctttcg cggcaattgt aagcgaaccg ccatggctga ccgctcgcta aagctatcga
     2401 gcaaaaatcg tcccgacgct gggacattcg acatcccgat tcaacgagca atttttggac
     2461 aaggtccacg caaaataatg cccatagcta gggactatag ccgcgcattc aatctttttt
     2521 atttgcgcgt ctcacgcgag cgaagcatgg ttcaagcgcc ccggacgctg gtgttcggtg
     2581 ggtgagtggg agaggtcaaa tggatcgtcg taacgttcct agtctgacga ggtgatcgag
     2641 tgaagcggcg cgagttcatc acgctgctcg gcggcgcggc ggccgcgtgg ccgctcacgg
     2701 cacgcgcggc taggctgcgg taaatccgtg gggagggatc acgatgcagc agatcgcgga
     2761 ctggctcgaa aagctcggcc tgtccgagta caccgcgcgc tttgtagaaa acgggatcga
     2821 tatctcggta ctgcgttatc tgaccgatca agatctcaag gacatcggtg tgctgcttgg
     2881 gcatcggcgc aaaatgctcg cggccatcag tgagctgacc ggccccatcc cggtaacaag
     2941 tgaagc
//
LOCUS       DATLJT010000073         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4138622,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000073 DATLJT010000000
VERSION     DATLJT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4138622"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VM910_01540"
     CDS             complement(<1..185)
                     /locus_tag="VM910_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI61268.1"
                     /translation="MGEVTTIGLDLAKHAFQVHGVDAEGATVLRKQLRRAQVLAFFSR
                     LPPCLLGLEACATAHYWA"
     gene            374..1065
                     /locus_tag="VM910_01545"
                     /pseudo
     CDS             374..1065
                     /locus_tag="VM910_01545"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
     gene            1039..>2061
                     /locus_tag="VM910_01550"
     CDS             1039..>2061
                     /locus_tag="VM910_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVI61269.1"
                     /translation="MGALTKARSSLSEGCHSIRQRFISGWRQWGKTLDIPDSPADIRR
                     EAYLSAVVLKVHQDRAYPGSIVASLSVPWGNSTDSIGGYHLVWPRDCVEAGLALLGVG
                     QIDDARSMLSYLIAIQNQDGSWNQNCFPDGRPFWVGIQLDEVGFPIILAAKLAEQDAL
                     RGLGGVGAMIRRAVCYLVHNGPISPQDRWEENSGISPFTLAIEIVALIGAAKFLSADE
                     RDYVISLADYWNERIEDWTYVSGGPFASQYGVDGYYVRIGPAAVQGGLCGRVNVANRW
                     GHSMPAIALIGMEYLHLARLGLRSPDDRRIQNTSKIADALLKVETPLGIAYHRYNEDG
                     YGEHADG"
BASE COUNT          419 a          611 c          632 g          399 t
ORIGIN      
        1 gcccagtagt gcgccgtcgc gcacgcctca aggccaagca gacagggcgg cagtcgactg
       61 aagaacgcca gaacctgcgc acgccgaagc tgcttgcgca gaaccgtcgc tccctctgca
      121 tcgaccccgt gcacctgaaa agcatgcttc gccaagtcca atccgatcgt agtaacctcg
      181 cccatggatg gctccattcg ctactgattc gatcagacag cagtttggca ctcagatgtc
      241 gcaagggagc catccaccca tcattcgatc acctgtcggc gcgggcgagc agggggctgc
      301 cccgatggga tggcaggact acaaagccga cgggcgccat ccgagaagga aaccgagaag
      361 gagcgggtga gcgatgacaa tcgcgatcgc cccgggaggt ccgggctccc gcccaacttg
      421 gacttcgagc gcgaaggaca tcgtgacgac cgggctcggc accagccggg tgtgggtcac
      481 gctgggctat ggcattctca acgaggtcta ctggccagca accgggctac cgcagattcg
      541 ggatctgggg ttcatcgtgg cgggaccatc cggttggttc gaggtgaaac gcgtcaaccg
      601 ctaccagatc tccataccag acgattggat gccgttaccg caggtcacgc accaaggcga
      661 ctgctatcgg ttggtgatcg aagtggttcc tgacccgcgc cgagacgtag ttctcatctc
      721 gtatcgtctc actggcgacg atgtgacgct ctacgcgctg ctggcaccgc atctcagcaa
      781 tggggcgaac acaacaacgt tcgcgccgca ggcgatttga cggcttggaa ggagcccgcc
      841 gcactgtgcc tgttcagcga ttgtggcttt tccaggtcga gtgcaggata cgtgggagct
      901 tccgacggat ggcaagattt ttccagaaac gggcgaatga cctgggaata tggcgaagcc
      961 attgacggca acgtagcgct gctcggcgag ctgccagcaa cgcaaggtac gcttgcgatc
     1021 gggttctccg acaccatcgt gggagccctc accaaggcac gctcgagcct cagcgaagga
     1081 tgtcactcga tacgccagag attcatctca ggctggcggc agtgggggaa aactctcgac
     1141 attccggata gcccggccga catccggcga gaagcttatc tgtcggctgt cgttctgaaa
     1201 gtgcatcagg accgcgccta tccaggttcg attgtcgcca gcctgagcgt gccgtgggga
     1261 aattccaccg attccattgg cggctatcat ttggtctggc cgcgcgattg cgtcgaagcg
     1321 ggcctcgccc ttttgggagt gggacagatc gatgatgccc gctccatgtt gtcctacctg
     1381 atcgcaattc aaaaccagga tggaagctgg aaccagaact gctttcccga tggtcgccca
     1441 ttctgggttg gcattcagct cgacgaagtc ggttttccca tcatcctcgc cgccaagctc
     1501 gcggaacaag atgcactgcg cggattgggt ggcgtgggtg ccatgatccg gcgcgctgtc
     1561 tgctatctcg ttcacaatgg cccaatcagc ccgcaagacc gctgggaaga gaattccggc
     1621 atcagccctt tcaccttggc aatcgagatc gtcgcactga tcggcgcggc taaatttctc
     1681 agcgctgatg aacgcgatta cgttatttca ctggccgact actggaacga gcggattgag
     1741 gactggacct atgtctcggg tgggccgttt gcctcccagt acggcgtcga cggctattac
     1801 gtgcggatcg ggccggccgc agtgcagggc ggcctgtgcg gacgcgtgaa cgtcgcgaac
     1861 cggtggggtc acagcatgcc ggcgatcgca ctcatcggga tggagtatct ccacttggcc
     1921 cgcctggggc tacgcagccc cgatgatcgc cgaattcaaa acacgtcgaa gatcgccgat
     1981 gcgttgctca aggtcgaaac accgctgggc attgcctatc atcgttacaa tgaagacggc
     2041 tatggtgagc atgcggacgg c
//
LOCUS       DATLJT010000074         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1611077,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000074 DATLJT010000000
VERSION     DATLJT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611077"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1501
                     /locus_tag="VM910_01555"
     CDS             20..1501
                     /locus_tag="VM910_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HVI61270.1"
                     /translation="MRFASFGLALCAFLLAPNEIGYQDIASLLARQPGVAERWQKRVY
                     SAAASVQLATYTFGRPIGTSAPQSATYRLASLTSEADITGSVPRNPILVAPPRYQASD
                     FPKVDRTLKGDRLVVEPQTPPEGAAPANNEPGLENPDASNASVAGAKTATAPAAAAPG
                     ANASAAEAPVSAPAAAVSSSARSPLDPELQAAMSAPPLPQYDISLSLEAQPLDDLNPA
                     PEATTIERSAARDGLNIKTANLFFGTSSLGGALEGMERWQPGEEPVVVMPDPDMKMMA
                     ALPPGSDAAKASESGESVAPKGEVNSDDQRTKTPAERLGLLDEKSRAKSEKCLAEAIY
                     FEARGEAVRGQIAVAQVVMNRAFSGYYPNTVCGVVYQNKYRHLACQFTFACDNNPDVI
                     REPDMWERAKKIAKATLDGQIWLPEVGKSTHYHAYYVHPSWVAEMKKLYKFGVHTFYR
                     PKAWGDGSDAPSWGTPAQTAALSEQLAQEAKSEAEMNPNSVRR"
     gene            complement(1528..2406)
                     /gene="nadC"
                     /locus_tag="VM910_01560"
     CDS             complement(1528..2406)
                     /gene="nadC"
                     /locus_tag="VM910_01560"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVI61271.1"
                     /translation="MTTPASLLHPDAFLSPLAIEEATTRALDEDLGRAGDVTSIATIP
                     ESAKAHAVLVARQAGVIAGLPLAIATFRKLSADILIQAYLRDGAAVAAGVHLLTISGP
                     ARAVLAGERTALNFVGRLSGIATLTADYVRHAAGTRMRICCTRKTTPGLRALEKYAVR
                     CGGGFNHRFGLDDALLIKDNHIAVAGGIRAVLERARAHAGHLVKIEIEVDTLDQLREV
                     LDTGIADAVLLDNMDIPTLTEAVGLAHGRVVLEASGGVTLSSIAKIAATGVDYASCGA
                     LTHSAPNFDVALDIDA"
     gene            complement(2416..4020)
                     /locus_tag="VM910_01565"
     CDS             complement(2416..4020)
                     /locus_tag="VM910_01565"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HVI61272.1"
                     /translation="MSNNIQNLLRSTDNIVIVGGGLAGLFCALKLAPRPVTLITAAPL
                     GEGASSAWAQGGIAAAVAEGDSAEAHAADTIAVGGGIVDEAIALGLAREAPDRIDDLL
                     RYGVPFDRDLEGRLAVGREAAHSARRIVHVQGDMAGKAIMAALTNAVRNTPSIRIIEG
                     FVAEALLAADGAVTGVQLRRTDDVTARPLTIATRALVLATGGIGHLYAVTTNPPEASG
                     LGLAIAARAGAVIADPEFVQFHPTAIMTGRDPAPLATEALRGEGAVLINAGGERFMPK
                     HHPLAELAPRDVVARGVFAEIRAGRGAFLDARAALGTRFAERFPTVYASCLEGGIDPA
                     TQPIPIAPAVHYHMGGIAVDTRGRTSLRGLWAGGEVSSTGAHGANRLASNSLLEAVVY
                     AARIADDIAGSVLPSPAPLPAEQPAPRNCAHPALAAKNLRTMMSSHVGVIRDGAHLAE
                     AVRAFAAIERDTGNIALRNMATSALLVAASALSRRESRGAHFRSDYPNEKPALARRTL
                     TTLNAARDIAKMLAETAREQTEPMIA"
     gene            complement(4075..>4803)
                     /gene="nadA"
                     /locus_tag="VM910_01570"
     CDS             complement(4075..>4803)
                     /gene="nadA"
                     /locus_tag="VM910_01570"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVI61273.1"
                     /translation="GVHFMAETSKILNPHKTVLIPDSRAGCSLASSITGADVRLLRER
                     FPAVPVVAYVNTSAEVKAEVDICCTSSNAVEVVESLNAPTVIFLPDQYLAKYVASKTD
                     VKIIAWKGACEVHERFTGDELRAYREADPSVQIIAHPECPPDVLAEADFTGSTAHMIN
                     WVRNNRSKRVVMITECSMADNVQAELPDVEMVKPCNLCPHMKRITLSNILDSLLYLRE
                     EVAIDPAIAERARRPIERMINLKN"
BASE COUNT          868 a         1554 c         1548 g          833 t
ORIGIN      
        1 gcgtaaccga ccgaaggacg tgcgttttgc gtccttcggt cttgctctct gcgccttctt
       61 attggcgccg aacgagatcg gctatcagga cattgcctcg ctgctggccc gtcagcccgg
      121 cgtcgccgag cgctggcaga aacgggttta ttccgcggcc gcatctgtcc agctcgccac
      181 ctatacgttc ggtcgcccga tcggaacgtc tgcgccgcaa agcgcgacgt atcggctggc
      241 cagcctgacc agcgaggctg atatcaccgg ttcggtaccg cgcaatccga tcctcgttgc
      301 gccgccgcgc taccaggcct cggactttcc gaaggtcgac cgcaccctga aaggcgaccg
      361 tctcgtcgtc gagccgcaga cccctccgga gggtgcagcg ccggcaaata acgagcccgg
      421 cctcgaaaat cccgacgcct cgaatgcctc cgtggcgggc gccaagaccg caacggcgcc
      481 cgcggccgca gcgcccggcg ccaatgcgtc cgctgcagaa gcgcccgttt ccgcaccagc
      541 ggctgcggta tcttcctccg cacgctcccc gctggatccg gaactccagg ccgcgatgag
      601 cgcgccgccg ctgccgcaat acgatatctc gctctcgctg gaagcccaac cgctcgacga
      661 tctcaacccg gcgcccgaag ccaccaccat cgagcggtcc gctgctcgcg acggtctcaa
      721 catcaagacc gcgaacctgt tcttcggtac gtcttcgctc ggcggggcgc tggaaggcat
      781 ggagcgctgg cagcccggcg aagagcctgt cgtcgtgatg cccgatcccg atatgaagat
      841 gatggcggcg ctgccgccgg gatccgacgc ggcgaaagct agcgaaagcg gcgagagcgt
      901 tgcgccgaag ggtgaggtga acagcgacga tcagcgtacc aaaacgccgg cggaacggct
      961 tggcctcctt gacgagaagt cgcgcgccaa atccgagaag tgcctcgccg aagcgattta
     1021 tttcgaagcc cgcggcgagg cggtgcgcgg ccagattgcg gtcgcgcagg tcgtgatgaa
     1081 ccgcgccttc tccggctact acccgaacac ggtgtgcggc gtggtttacc agaacaagta
     1141 tcggcacctt gcctgccagt tcaccttcgc ttgcgacaac aatcccgacg tcatccgcga
     1201 acccgacatg tgggagcgcg caaagaagat cgccaaagcg acgctcgacg gccagatttg
     1261 gctgcccgag gtcggcaagt cgacgcacta tcacgcctat tacgtgcatc cctcctgggt
     1321 cgccgaaatg aagaagctct acaaattcgg cgtgcacacc ttctaccggc cgaaagcctg
     1381 gggcgacggc agcgacgcgc cgagctgggg aacgccggcg cagactgcag ccctttcaga
     1441 gcagctcgcc caggaggcaa agagcgaagc ggagatgaac ccgaactccg ttcggcgata
     1501 gggtgagggg agacggtcgg acaatcttca ggcgtcgata tccagcgcca cgtcgaaatt
     1561 cggcgccgaa tgcgtgagtg cgccgcaaga ggcatagtcc acgccggttg ccgcaatttt
     1621 cgcaatcgag ctcaacgtaa cgccgccgga agcttccagc accacgcggc catgcgcaag
     1681 ccccaccgcc tccgttagcg tcggaatatc catattgtcg agcagcaccg catcggcaat
     1741 gccggtatcg agcacctcgc gcaactgatc gagcgtgtcg acctcgatct cgatcttgac
     1801 gagatggccg gcatgggcgc gggcgcgttc cagcaccgcc cggatgccgc cggcgaccgc
     1861 gatgtggttg tccttgatca ggagcgcgtc gtcgagcccg aagcggtgat tgaagccgcc
     1921 gccgcaccgc accgcatatt tctccaatgc gcgcaagcca ggtgtggttt tccgcgtaca
     1981 gcagatgcgc atcctggtgc cggcggcatg cctgacatag tccgcagtga gcgttgcgat
     2041 cccggacagc cggccgacaa aattgagtgc ggtgcgttcg ccagcgagca cagcacgcgc
     2101 gggtccggag atcgtcagca gatggacgcc ggctgccacc gctgcgccgt cgcgaagata
     2161 ggcctgaatg agaatatcgg ccgagagttt ccgaaacgtg gcgatcgcaa gcggcagccc
     2221 cgcgatcacg cccgcctgtc gcgccacaag aacggcatgc gccttggcgc tttccggaat
     2281 ggtcgcgatt gaggttacgt cgccggcgcg gccaaggtct tcgtcgaggg cgcgggtggt
     2341 cgcttcctcg atcgcaagcg gcgacaggaa cgcgtccggg tgcaggagcg aagcaggggt
     2401 ggtcattgag gtcctctagg cgatcatcgg ctctgtttgc tcgcgcgcag tctcggcgag
     2461 catcttcgcg atgtctcgcg cggcattgag cgtcgtcagg gtgcgcctgg cgagcgccgg
     2521 tttctcgttt ggatagtcgg aacgaaagtg cgcgccacgg ctttcgcgcc ggcttagggc
     2581 ggatgccgcg accaacagtg ccgatgtcgc catgttgcgg agcgcaatgt tgccggtgtc
     2641 gcgctcgatc gctgcaaagg cccgcacggc ttccgcaagg tgcgcgccgt ccctgatcac
     2701 gccgacatgc gagctcatca ttgtgcggag atttttggcg gcaagcgcgg ggtgagcgca
     2761 attgcgagga gcaggttgct ctgccggcag aggggcaggg gacggcagca cactgccggc
     2821 aatgtcatct gcgatgcggg cggcatagac gaccgcttcc aggagcgagt tcgaggcgag
     2881 ccgattggcg ccatgcgcgc cagtggatga gacctcgccg ccggcccaga gaccacggag
     2941 cgaagtgcgg ccacgtgtat ccaccgcgat gccgcccatg tgataatgca cggcgggtgc
     3001 aatcggtatc ggttgcgttg ccggatcgat gccgccttca aggcaactcg catacacggt
     3061 cgggaatctt tccgcaaagc gcgtgccgag tgctgcgcgc gcatcgagaa acgcgccgcg
     3121 accggccctg atctcggcga acacgccgcg cgcgaccacg tcacgcggcg caagctcggc
     3181 aagcggatga tgtttcggca tgaagcgttc gccgccggca ttgatcagta cggcgccttc
     3241 gccgcgcaag gcctcggtcg caagcggcgc cggatcgcgc cccgtcatga tggcggtcgg
     3301 gtggaattgc acgaattcgg gatcggcaat gaccgcgccg gcacgcgccg cgatcgccaa
     3361 tcccagcccg cttgcttcag gcggattggt tgtcactgca taaagatggc cgatgccgcc
     3421 ggttgccagc accagcgccc gcgttgcgat cgtcaggggt cgcgcggtga cgtcgtccgt
     3481 cctgcgcaat tgcacgcccg ttaccgcgcc gtcggcagcg agcagcgctt cggcgacgaa
     3541 accttcgatg atgcggatcg agggtgtatt gcgcacggca ttcgtcagcg ctgccatgat
     3601 cgctttgccc gccatgtcgc cctgcacatg gacgatgcgt cgagccgaat gcgcagcctc
     3661 gcggccgacc gcgagccggc cttcgaggtc gcgatcgaag ggaacgccgt agcgcaagag
     3721 atcgtcgata cggtcgggcg cttcgcgcgc aagtccgagc gcaatcgctt catcgacaat
     3781 gccgccgcct accgcaatcg tgtcggcggc atgcgcttcc gcgctgtcac cttcggcgac
     3841 ggctgcggcg atgccgccct gcgcccaggc gctggaggca ccttctccga gcggggccgc
     3901 ggtaatcagc gtaacgggcc gtggcgcgag tttcagcgcg caaaacaatc cggcaagccc
     3961 gccgccgacg atgacgatgt tgtcggttga gcggaggaga ttttggatat tgtttgacat
     4021 ggctggttcc tcgtcgtcgc tctgctcgcg ttgctcgcca ggcggacgac ggagctagtt
     4081 tttcaaattg atcatccgct cgatcggacg ccgcgcacgt tcggcgatcg cgggatcgat
     4141 cgcgacctcc tcacgcagat aaagcaggct gtccagaatg ttcgacagcg tgatccgttt
     4201 catgtgcgga cagagattgc agggcttcac catctcgacg tcgggcagtt cggcctgcac
     4261 attgtcggcc atcgagcatt cggtgatcat caccacgcgc ttgctgcgat tgttgcgcac
     4321 ccagttgatc atgtgcgccg tggaccccgt gaaatccgct tctgccagca cgtcgggcgg
     4381 gcattcgggg tgcgcgatga tctgcacgga agggtccgcc tcgcgatagg cgcgcaactc
     4441 gtcgccggtg aagcgctcat gcacctcgca ggcgcccttc caggcgatga tcttcacatc
     4501 ggtcttcgag gccacatatt tcgcgagata ctggtcgggt agaaagatta cggtcggcgc
     4561 attcaggctc tcgaccacct cgaccgcatt cgacgatgtg cagcagatat cgacttccgc
     4621 tttcacctcg gccgacgtgt tgacgtaagc gacgaccggc acggcgggaa aacgctcgcg
     4681 caacagccgc acatcggcgc ccgtgatgct cgaagccagc gagcatccgg cgcgcgaatc
     4741 cgggatcagt accgtcttgt gcggattgag gatcttcgag gtctccgcca tgaagtgaac
     4801 gcc
//
LOCUS       DATLJT010000075         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_922118,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000075 DATLJT010000000
VERSION     DATLJT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922118"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VM910_01575"
     CDS             <1..832
                     /locus_tag="VM910_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI61274.1"
                     /translation="GSPNIYYGSSRGGGYSVAARGGNTAQTEQADREALAETNTAAVE
                     SCTGLAPGVTNLPIDQIRQTVHPTADQEAALDDLSGASTRASDVIKSSCPSSVPLTPI
                     GRLDAAEQRLDSTIKAIQVVRSPLERFYEALNDEQRQRFNAMNGSTRGAPSAGKMAAA
                     CNQQTGIDLPAQRIEQVVQPTAQQQSAFDDLKKAAQKAGDQLQASCPTAVPKSPVARV
                     DTVETRLTAMADAIKSVRPGLQNFYASLSDEQKAKFNTMGPPPNTTSSPQQRQTGGQQ
                     "
     gene            976..1917
                     /locus_tag="VM910_01580"
     CDS             976..1917
                     /locus_tag="VM910_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI61275.1"
                     /translation="MRKIGAILGITALATVIFSAPPAAAFSIHLGSFYFHVPFVGHHH
                     RHHPANDARTKQSDVSRGGAAKIEQADREARVETNTAAIESCTGLAPGVTNLPIDQIR
                     QTVRPTADQEAALDDLSAASSQASDIIKSSCPSSVPLTPIGRLDAADQRLDATVKAIQ
                     IVRSPLERFYQALSDEQRQRFNAMGAPSAGNMAAACSQQRGSFVDLPVQRIEQIIQPT
                     AQQRSTFNDLKKATQSATDHLRSSCPTAVPLSPVTRLDTIETRLRAMADAIKSVRPDL
                     QNFYASLNDEQKAKFNTMGPPPNTTSSPQQRQSGGQQ"
     gene            complement(2147..2347)
                     /locus_tag="VM910_01585"
                     /pseudo
     CDS             complement(2147..2347)
                     /locus_tag="VM910_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2376..3035)
                     /locus_tag="VM910_01590"
     CDS             complement(2376..3035)
                     /locus_tag="VM910_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HVI61276.1"
                     /translation="MSLDFAECDRARLARDAAYDGLFYTGVHTTGIYCRPVCPVRPAR
                     SANVSFFPSAAAAEVAGFRPCLRCRPETAPFCRAWIGSRATVERALRLIAEEGALDQE
                     GATVERLAQRLGMGSRQLTRLFARHLQASPSKVARTARVQRAKRLIDESRLPMTEIAM
                     RAGFGSLRRFNSAFAEIYGRPPTEIRRARRSARKEDPTVENVVIGPPTPARPSTLAQR
                     A"
     gene            3254..>3994
                     /locus_tag="VM910_01595"
     CDS             3254..>3994
                     /locus_tag="VM910_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI61277.1"
                     /translation="MTGGYRKTPVLQIGADIYCDSQLIMRELERRHPTPSFYPAGHGA
                     ADALAWWAEKTTFSPAVSIAFAKRRDALPEGFLEDRAKFSGRNIDPAAMMAAVPNLLD
                     QLRAHFDWLNQMLIDERSFLQGSAASLADLAAYHPIWFLKQNFGPTAVPLDGFPRLLS
                     WTERIAAIGHGRRSPTTSKKALDAARDATSIASVIPDPRDPIGRKPGQTVTVTPDDTG
                     RDPVVGELVASGVHEIVIRRSDPAVGEVC"
BASE COUNT          824 a         1262 c         1176 g          732 t
ORIGIN      
        1 cggttcaccc aacatctact acggcagctc tcgtggcggt ggctatagcg tcgccgcccg
       61 cgggggtaac accgcccaga ccgagcaggc ggatcgcgaa gcactcgccg aaaccaatac
      121 cgcggcggtc gaaagttgca ccggcctagc acccggtgtg accaatctgc cgattgatca
      181 aatccgacaa accgtccatc cgacagctga tcaggaagcg gctctcgacg atctcagcgg
      241 agcatcgacg cgagcaagcg atgtcatcaa gtcgtcgtgc ccgagttcgg ttccgctcac
      301 accgattggt cgcctcgatg ccgccgagca acgactggat tcgaccatca aagccataca
      361 agttgtccgc tcgccacttg agaggtttta cgaggcgctg aacgatgaac agaggcagcg
      421 gttcaacgcg atgaacggct cgaccagggg cgctccttcg gccggcaaaa tggcggccgc
      481 atgcaatcag cagacgggca tcgacctgcc ggcgcaacgc attgaacagg tggttcagcc
      541 gactgcgcaa cagcagagcg ccttcgatga tctcaagaag gccgcgcaaa aggcgggcga
      601 ccagctgcaa gcatcgtgtc cgaccgcggt gccgaaatcg ccagtggcgc gggtcgatac
      661 agttgaaaca cggctaaccg cgatggcgga cgccatcaag tcagttcgcc ctggccttca
      721 gaacttctat gcttcgttga gcgacgagca gaaggcaaag ttcaatacaa tgggcccgcc
      781 gcctaatact acttcgtcgc cacagcagcg tcagaccggc ggccagcaat agccgaatgc
      841 cttcgcgaac aataagcagc cactgccatt acctcaatgc cgctcaggtt cgccgcaatc
      901 tataagagca agaaggggca agttgttcgt tgttttaacc tctgtggcca ccccttctac
      961 ataagttgcc gtgtcatgcg aaagatcggc gctatcttag ggataaccgc actcgccacc
     1021 gttattttct ccgctccgcc agccgcggcg tttagcatcc atctcgggtc gttttatttc
     1081 cacgtacctt ttgtcgggca ccatcaccgt catcacccgg caaacgatgc tcgaactaag
     1141 cagagcgacg tctcgcgcgg cggtgctgcc aagatcgagc aggccgatcg tgaggcgcga
     1201 gtcgaaacca atactgcggc gatcgaaagt tgcactggcc tagcgccggg cgtgaccaat
     1261 ctgccaatcg atcagatccg acaaaccgtc cgtccgacag ctgatcagga agcggctctc
     1321 gacgatctca gcgccgcgtc gtcgcaagcg agcgatatca tcaagtcgtc gtgcccaagc
     1381 tcggttccgc ttacaccgat tggccgcctc gacgccgccg atcagcgact ggatgcgact
     1441 gtcaaagcga tacaaattgt ccgctcaccg cttgaaaggt tttaccaggc gctgagcgat
     1501 gaacagaggc agcggttcaa cgcgatgggc gctccttcgg ctggtaatat ggcggccgcg
     1561 tgcagtcagc aaagaggcag cttcgtcgat ctgccagtgc aacgcattga acagatcatt
     1621 cagcccactg cgcaacagcg aagcaccttc aacgacctta agaaagcaac acaaagcgct
     1681 accgaccatt tacggtcgtc ctgtccaact gcagtgccgc tatcgccagt gactcggctc
     1741 gatacgatcg agacacggct aagagctatg gcggacgcaa ttaaatcagt tcgccctgac
     1801 cttcagaact tctacgcctc gctgaacgac gagcagaagg caaagttcaa tacaatgggc
     1861 ccgccgccta atactacttc gtcgccacag caacgtcaga gcggcggcca gcaatagcgg
     1921 acgcctcttt ggaatagcct cggctacccg ctgcgggttg catcagcggc ttgagtgacg
     1981 tacccgcagc ttgtgatgaa gggcaggaag gaggattttt cctggcccct cctggcaccg
     2041 cgcagccctc gcttccttcc atctcgccgc aaagactatc ggtaattgac caggcggcaa
     2101 tcattccgcc acgtctcgat gccgcggcct catttgaagt tcgcggttac gatgcaccgg
     2161 ccttgccgat gcggtcgatc aggtcgagaa gctccctggt cgagcggcca agccggtcaa
     2221 gccggtcaag cttggtcacg gcgacgacat cgccagcttt aagcccggcc ataagcttcg
     2281 cgagttgcgg gcgatcagcg cgggcgccgc tcactttctc gcggaagata gtgccagcgc
     2341 cggcagcctt gagcgcgtca acctagccgg tcaggttacg cccgctgcgc gagagtcgac
     2401 gggcgtgccg gcgttggcgg tccgatgacg acattctcga ccgttgggtc ctctttgcgc
     2461 gctgaccgcc gcgcacgccg aatctcggtc ggcggcctgc cgtagatttc ggcaaaggcg
     2521 gagttgaacc gtcgcaggct tccgaagccc gcccgcatcg caatctcggt catcggcagg
     2581 cgggactcgt cgatcaggcg tttggctcgc tgcacacgcg cggtccttgc cacttttgaa
     2641 ggactagcct ggagatggcg cgcgaataat cgcgtcagtt gccgtgaccc cataccgagg
     2701 cgctgggcga ggcgctccac agtggctccc tcctgatcga gtgctccctc ctcggcgatg
     2761 agccgcagtg cccgctcgac cgtcgcccgg ctgccgatcc aggctctaca aaatggagcg
     2821 gtttccggcc ggcagcgcag gcacgggcgg aagccggcca cctccgcagc ggcggccgac
     2881 gggaagaacg aaacgttggc tgaccgggcg gggcgcaccg gacagaccgg acggcaatag
     2941 atgcccgtgg tatggacgcc ggtatagaac agcccgtcat aggccgcgtc tcgcgccaag
     3001 cgcgcccgat cacattccgc aaagtcgagc gacatgagag cgataatact cgctgccgct
     3061 cctctgccag acactctttc ggggatggag tccggttttg gccagcaaca gcggcttggt
     3121 tccactacat ctaattgcga gcgctcaggg gccgtctcga tgactgatat catcctgcat
     3181 cactacgaga cctctcccta ttcggagaaa gttcggctcg gtcttggtct gaagggcttg
     3241 gcctgggggg gcgctgaccg gaggctaccg caagacgccc gtgttgcaga tcggcgcaga
     3301 catctattgc gatagccagt tgatcatgcg cgaactcgag cgccggcatc ccaccccgag
     3361 cttttatccc gccggccacg gtgcggccga tgcgcttgcc tggtgggccg aaaagacaac
     3421 gttcagcccg gctgttagca tcgcgttcgc gaagagacgc gacgcgttgc ccgagggatt
     3481 tctcgaagac cgggccaaat tctccggccg aaatatcgat ccggccgcga tgatggcggc
     3541 agtaccgaac ctgctcgatc agttgcgcgc gcatttcgat tggctgaatc agatgctgat
     3601 cgacgagcgc tcattcttgc agggctctgc cgctagcctt gccgatctcg ccgcctacca
     3661 tccgatttgg tttttgaaac aaaatttcgg gccaacggcg gtgccgctcg acggctttcc
     3721 gaggctgctg agttggaccg agcgcattgc cgcaatcggc cacggcaggc ggagcccaac
     3781 gacctcgaaa aaagcgctcg atgcggcgag ggacgctacg tcgatcgcca gcgtaatccc
     3841 cgatccgcga gatccgatcg gccggaaacc tggtcagaca gtgaccgtca cgccggatga
     3901 taccggacgc gacccggtcg tcggcgagtt ggtcgcctcg ggcgtccatg agatcgtcat
     3961 ccgacgcagc gatcccgcgg tcggagaggt ctgt
//
LOCUS       DATLJT010000076         4548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3908992,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000076 DATLJT010000000
VERSION     DATLJT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908992"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VM910_01600"
     CDS             <1..343
                     /locus_tag="VM910_01600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI61278.1"
                     /translation="QTLGESLPTLRAQLATMQIDTDGLAARIDDVVDHLERPRDTTML
                     RVLESKAAAGDVAIAVTSAAMRVCGGAAFSKHLAIERLFRDAHAGAVMAPTGDVLRDF
                     VGKAILGLPLF"
     gene            455..1294
                     /locus_tag="VM910_01605"
     CDS             455..1294
                     /locus_tag="VM910_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HVI61279.1"
                     /translation="MNRTIWVGAVAYDPKVVSIWEGMRRYFHEEAGLPVEVVLFQSYE
                     AQVAALLALPGEPLPRIDIGWNTNLAFIQADAWSDHRCRPIAMRDTDVGWMTKIVAVT
                     GGPVAKLADIKNRTLALGSRDSGHAAILPVYFLQREGLVEEKDYRTLRFNNDVGKHGD
                     TGRSEADVVRAVLDGRADAGAIGSPFWNAVRSERLVPEGALTEIWTSQPYNHCMFTAR
                     SDLDPALEQAFAKALFAMSYDNPTHRAVLEAEGLRQWIAPQLEAYAELRNASAQQGFL
                     RRP"
     gene            complement(1314..2342)
                     /locus_tag="VM910_01610"
     CDS             complement(1314..2342)
                     /locus_tag="VM910_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI61280.1"
                     /translation="MRTNADVELDAGTKTFASGLLPDLIDTLRRSRQGDLVAVTSGDP
                     TIGPELEAWCRFTRNSLVDVAVQEGHTRWVFRYGEAPEQEDRPVGSRLWLYTNFDCNL
                     CCDYCCVRSSPKAPRRALGIERVRRIAREAAELGVSEIFVTGGEPFMLPEIGEILAVC
                     AAAAPTTVLTNGMLFAGRRLEMLRSLPRERVKLQISLDSPTPERHDSHRGKGTWARTR
                     EGIERARAEGFRVRLAATVSTDAEAEEFRCFLDAHHVSEEDRVIRRIALRGFATQGIA
                     LAKADLVPEVTITAEGVFWHPVGAEDNDLLVSREIFPLTDSFAAVRRAFDREFDHERR
                     LARIFNCA"
     gene            2804..3226
                     /locus_tag="VM910_01615"
     CDS             2804..3226
                     /locus_tag="VM910_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HVI61281.1"
                     /translation="MIGPAFAPSALPRAAAVLVLLAAGFALSGCSDMNESLTTVFADP
                     AKYELWDCKQLEPERKRLANRAAELQGLMAKAETGAAGPVVAELAYRNDYVAVRGQSH
                     YAEEAWRRNKCHETAPAAESPPASVKPAPSSKAGRAVY"
     gene            complement(3231..4433)
                     /locus_tag="VM910_01620"
     CDS             complement(3231..4433)
                     /locus_tag="VM910_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI61282.1"
                     /translation="MAASRTIFVAGAGIGGLTASLALAARGFRVVVLEKAERLEEAGA
                     GLQVSPNASRVLVDLGLKDRLGRRAITPEAISIMSARAGREIVRLPLGEEAAARAGAP
                     YWVMHRADLQAALYAAVNEHPDIDLRFGWQFEDAAAHAKGLTIVQRSGNARHQELALA
                     LVGADGIWSSVRHHLFPEAQPQFSGLIAWRGTLDATQLPREYTAPRVQLWMGPGAHLV
                     AYPISGARQINVVAIVPGTWNRPGWSVPGDGVELKAAFDASRWPGPARMLIGAVDIWR
                     RWALFTVQEDGEWTSGSIALLGDAAHAMLPFAAQGAGMAIEDAAVLAKILSQSPLDNA
                     NQVAAALKRYAKLRRPRVAQVQRAARSLGRTYHLTGPFALARDVAIRAIGAQRMMERQ
                     SWIYDWQA"
     gene            complement(4441..>4548)
                     /locus_tag="VM910_01625"
     CDS             complement(4441..>4548)
                     /locus_tag="VM910_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HVI61283.1"
                     /translation="DSEIICPYCSTLYRFASDLGAGEARPPECVLKDVA"
BASE COUNT          831 a         1468 c         1455 g          794 t
ORIGIN      
        1 ccagaccctc ggagagagcc tgccgacgct tcgtgcgcaa cttgcgacga tgcagatcga
       61 cactgacggc ctagcggcgc gcatcgacga tgtcgtcgat cacctcgagc gccctcggga
      121 taccaccatg ttgcgcgtgc tcgaaagcaa ggcggcggca ggtgatgttg ccatcgctgt
      181 cacctcggcg gcgatgcggg tgtgcggagg tgcggcgttt tccaagcatc tggcaatcga
      241 gcggttgttc cgcgatgccc atgcgggcgc tgtcatggca ccgacgggcg acgttttgcg
      301 ggacttcgtc ggcaaagcca ttttgggact accgctgttc tagcgatcga atcctgacat
      361 tcgcatccca ttcggggagc cacccgcgag tgtcgggatc aaaggaccac tggcaagttg
      421 tgtagcggtt gcaagcgtga caggtgaggc gatcatgaac cggacgatct gggtaggtgc
      481 cgtcgcttat gatccgaagg tggtcagcat ctgggagggc atgcggcggt actttcacga
      541 agaagcgggc ctccctgtcg aggtggtact gtttcagagc tacgaggcgc aggtcgccgc
      601 ccttctcgca ttgcccggcg agccgctgcc gcgaattgac atcggatgga atacgaacct
      661 cgctttcatc caggccgatg cctggagcga ccaccgttgc cgaccgatcg ccatgcgcga
      721 tacggatgtg gggtggatga ccaagatcgt cgcggtcacc ggagggccgg tggcgaagct
      781 tgccgatatc aagaaccgca cgctcgcgct cggcagccgc gacagcggcc acgcggcaat
      841 cctgccggtt tattttctgc agcgcgaagg actggtcgag gaaaaggact accgaacatt
      901 gcgcttcaac aacgacgtcg gcaagcacgg tgacaccgga agaagcgagg cggatgtcgt
      961 tcgcgcggtg ctcgacggcc gcgccgatgc cggcgcaatc ggcagtcctt tctggaatgc
     1021 cgtacgcagc gaacgactgg tgccggaagg cgcgcttacc gaaatctgga cctcgcagcc
     1081 ctataatcac tgcatgttca cggcgcggtc ggatctcgac ccggcattgg agcaggcgtt
     1141 tgccaaagca ctcttcgcga tgagctacga taacccgacg catcgcgccg tgctcgaggc
     1201 ggaagggctg cgccaatgga tcgcgccgca gcttgaagct tatgccgagc tgcggaatgc
     1261 gtcggcgcag caaggctttc taaggcggcc gtaagctgca cgctccgcga cgatcaggca
     1321 caattgaaaa tcctggccaa ccggcgctca tgatcgaact cgcgatcgaa ggcgcggcgc
     1381 acggccgcga aggaatcggt gagcggaaag atctcgcggc tgaccagcag gtcgttatcc
     1441 tcagcgccga ccggatgcca aaacacgcct tccgccgtaa tcgtcacttc cggaacgaga
     1501 tctgcctttg ccagggcgat gccttgcgtg gcgaagcccc gcagggcaat tcgcctgatc
     1561 acccggtcct cctcgctgac gtgatgggcg tcaaggaaac agcggaattc ctcggcctcg
     1621 gcgtcagtcg acaccgtcgc cgccaaccgc acgcggaatc cttcggcgcg ggcgcgctca
     1681 attccttcgc gtgtccgtgc ccatgttccc tttccgcgat ggctgtcgtg gcgctccggc
     1741 gttggactgt cgaggctaat ctgcagctta acgcgctcgc gcggcaatga acgcagcatt
     1801 tcgagccgac gcccggcaaa cagcatgccg ttggtgagca ccgtggtcgg cgctgccgca
     1861 gcgcatacag caaggatctc gccgatctcc ggcagcatga acggctcacc gccggttaca
     1921 aaaatctcgc ttacgccaag ctccgccgcc tcgcgggcga tccgccgcac ccgctcgata
     1981 ccaagagccc gtcgcggagc cttgggtgac gagcgcacgc aacaatagtc gcagcagagg
     2041 ttgcagtcga agttggtgta aagccacaag cgagagccga caggccgatc ctcttgttcg
     2101 ggcgcctctc cgtatcgaaa cacccagcgg gtgtgccctt cctggacggc tacatcgacc
     2161 aaactattgc gggtgaaccg acaccatgcc tcgagttccg ggccgatggt cgggtcgccg
     2221 ctggtcacgg ccacgaggtc gccttgacgg ctgcggcgca gcgtgtcgat caaatccggc
     2281 aggaggcccg aagcaaatgt cttggtgccg gcgtcgagtt caacgtccgc gttcgtcctc
     2341 atggacagaa ctatagtttt cgcttcaaca aatccaagag gtctggggcg tcgtgatggg
     2401 gcctctgatc gttcgcgtat tgcatgcaag tccggccaac cctggcgaac gtttgcgcga
     2461 gcaatccgtg tggtcgacga ttacgtggaa gcaaaggaaa gctttagcct tcctcggcga
     2521 gaccttgccg tgcctgacgc tcagatcgcc tgccgctcac cgtccagtcg cgagcaaatc
     2581 gtggcaatcg caacgttgtt tgagccataa atccgatacc cggcttttcc ggatcatgcc
     2641 tgatagatca aatacttagc tggctttctg gcggtctgga atgaagggca gtcgggcgga
     2701 agggcgccct tgctcacgtc gcaccaagtt gccggaaaag cctcaatccg gccaagagcg
     2761 cctgctttag ccaatctcaa tctcctcggt ctagcaaagc cttatgatcg gacccgcttt
     2821 cgcaccttcc gctttgccgc gcgcggccgc cgtcctcgtg ctgcttgcgg cgggctttgc
     2881 gctctcgggc tgctccgaca tgaacgagag cctgacgacg gtgtttgccg atcccgccaa
     2941 atacgaactc tgggattgca agcagctcga gccggagcgc aagcgcctcg ccaatcgcgc
     3001 ggccgaattg cagggcctga tggccaaggc cgagaccggc gccgcgggtc ccgtggtggc
     3061 ggagctagcc taccgaaacg attatgtcgc ggtgcgcggc caatcgcact atgccgaaga
     3121 ggcctggcgt cgcaacaaat gccacgaaac ggcgccggcg gccgagagtc cacccgcaag
     3181 cgtcaagccc gcaccgtcct ccaaggccgg aagggccgtg tattagggac tcatgcctgc
     3241 cagtcgtaga tccagctctg ccgctccatc atgcgttgcg ccccgatcgc cctgatggcg
     3301 acgtcgcgcg cgagcgcgaa ggggccggtg aggtgatagg tgcgtccgag gctgcgggcg
     3361 gcgcgctgca cctgcgccac gcgcggccgg cgcagctttg cgtaacgctt caaggcggcc
     3421 gcaacctgat ttgcgttgtc cagggggctt tggctcaaaa tcttggcgag caccgcggcg
     3481 tcctcgatcg ccatgccggc gccttgcgcc gcaaatggca gcatcgcgtg cgcggcgtcg
     3541 ccgagcaggg caatgctgcc gctggtccac tcgccgtcct cctgcaccgt gaacagcgcc
     3601 cagcggcgcc agatatcgac ggcgccgatc agcatccgcg ccgggcctgg ccaccgtgac
     3661 gcgtcgaacg cggccttcag ctcgacccca tcgccgggca cgctccagcc cggcctgttc
     3721 catgtgcccg gcacgatcgc gacaacgttg atctggcgcg cgccggagat cgggtaggcg
     3781 acgagatgcg cgcccggtcc catccagagt tgcacccgag gcgccgtata ttcgcgcggc
     3841 aattgcgtgg catcgagcgt tccgcgccac gcgatcagcc cggaaaattg cggttgcgct
     3901 tcggggaaca gatgatggcg gaccgaggac cagattccgt cggcgccgac cagcgccagc
     3961 gccagctcct gatggcgcgc attgccgctg cgctggacga tcgtcagccc cttggcgtga
     4021 gcggctgcgt cctcgaattg ccagccgaac cgcagatcga tgtcgggatg ctcgttgacg
     4081 gcggcgtaaa gtgcggcctg cagatcggcc cgatgcatca cccaataggg tgcgccggcg
     4141 cgcgcggccg cctcctcgcc gagcggcagg cgaacgattt cgcggccggc ccgggcgctc
     4201 atgatgctga tcgcttcggg ggtgatggcg cgccgcccga ggcggtcctt caggccgagg
     4261 tcgacaagca cgcggctcgc gttgggggaa acctgcagtc ccgcaccggc ttcttcgagc
     4321 cgttcggcct tctccagcac cacaacacga aacccgcgcg ccgcgagcgc cagcgaggcg
     4381 gtcaggccgc cgatcccggc gcccgcgacg aaaatggtac gcgacgccgc caccgtctaa
     4441 tcaggcgaca tccttgagca cgcattctgg cggccgggct tcgcccgcgc caaggtcgga
     4501 cgcaaaacga tacagcgtgg agcaataggg gcagatgatt tcgctgtc
//
LOCUS       DATLJT010000077         1734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2760211,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000077 DATLJT010000000
VERSION     DATLJT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760211"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1267
                     /locus_tag="VM910_01630"
     CDS             281..1267
                     /locus_tag="VM910_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61284.1"
                     /translation="MRRREFITLLGGAAVAWPFPVRAQQPPTTVKQARVGVLAVSPPT
                     PAMLNAFREGMRERGYIEGQNLSIDVRWSQGTFEQISALAAELVSANVQVIIAWATPS
                     VIAARRATSVIPIVMVSVGDPVGSGFVTSLARPGGNITGFSGFTADLSAKLMALFVEL
                     VPNIKRVGIVSNSYNPNVMVQLWETEDAVHKLGLQSQVVEARTPEEFERAFARLSMER
                     VHGVVLLADPSVIEQAKKIAELAQAARLPTAFQRRENVEAGGLMSYGGNIQYQFRQAA
                     IHVDRILKGAKPADLPVEQPTKFELVVNLSAAKALGLSIPESFLSLADEVIE"
     gene            1264..>1734
                     /locus_tag="VM910_01635"
     CDS             1264..>1734
                     /locus_tag="VM910_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61285.1"
                     /translation="MKRRAFITLLGGAAAWPITARAQQPERRRRIGVLMNVAANDPQG
                     QVRVAAFQQVLQQLGWSEGRNARIDTRWGENDVEYERQYAAELVTLAPDVMLASGTQS
                     VAALQRATRTLPIVFVGVADPVGAGLIDSLARPGGNTTGFMEFEYSFSGKWLELL"
BASE COUNT          328 a          527 c          551 g          328 t
ORIGIN      
        1 gcgataccgt caacatcgcc gcgcgtctcg aggctgccgg tcagcccaat cgcgtgctcg
       61 cgtccgcgac gaccgtgaaa gggctgggaa gcgattacag cttcgatggt ccacacaaga
      121 tcgacaccaa ggaaggccgc gtgctggagg cgcttttcgt aagtcgtcac ccttgaccgt
      181 agctcgcatc tttcggtcag gaagctactt tatcaagggc atcaacccga ctcggacatg
      241 ggcgtcatcc ggcaaggttc tcttgagact ggagcagcaa atgagacggc gcgagttcat
      301 caccctgctt ggcggtgcgg cagtcgcgtg gccgttcccg gtgcgcgcgc agcagccgcc
      361 cacgacggtt aagcaggccc gcgttggagt gctcgcggtt tcgcctccga cgcccgcgat
      421 gttgaacgct ttccgcgagg gtatgcgcga acgcggctat atcgaaggac aaaacctgtc
      481 cattgacgtt cgctggtcgc aagggacctt cgagcaaatc tccgccctcg cagctgaact
      541 cgttagtgcc aacgtccagg tcataatcgc atgggccacg ccgtcggtga tcgcggcccg
      601 ccgcgcaacg tccgtaattc ccatcgttat ggtgtcggtc ggcgatccgg tgggttcggg
      661 gttcgtcacc agtctggcgc gacccggtgg aaacatcacc ggtttcagtg gcttcacggc
      721 cgatctcagc gccaaactga tggcgctatt cgtcgagctt gtccccaata tcaagcgcgt
      781 cggtatcgtg agtaattcct ataacccgaa cgtgatggtg caactttggg agaccgagga
      841 cgccgttcat aaattgggct tacagtcgca ggtcgtcgag gcgcgcacgc ctgaggaatt
      901 cgagcgcgca ttcgctcgtc tgagcatgga gcgtgtccac ggcgtcgtgc tgcttgcgga
      961 cccctcggtc atcgaacagg cgaagaaaat cgcggagctt gcgcaggcgg ctcggttgcc
     1021 gactgcattt cagcgccgcg agaacgtcga ggcgggcggc ctgatgtcat atggagggaa
     1081 tattcaatat cagttccgcc aggcggcgat ccatgtcgac cgcattctga aaggtgccaa
     1141 gcccgccgac cttccagtgg agcaaccgac caagttcgaa ctcgtcgtca atctttcggc
     1201 cgccaaagca ctcgggctga gcatcccgga atcgttcctg tcactcgccg acgaggtgat
     1261 cgaatgaagc gccgcgcgtt catcacgctg ctcggcggcg cggcggcgtg gccaatcaca
     1321 gcgcgcgcgc agcagccgga gcgcaggcgg cgcattggcg tgctcatgaa cgtggcggct
     1381 aacgatccac aaggtcaggt acgtgtcgcg gcgttccagc aagtcctgca acaactgggc
     1441 tggagcgagg gtcgcaatgc gcggatcgac acgcgctggg gcgagaacga tgtcgaatac
     1501 gagcgccaat acgcggcgga attggtcacg ctcgccccgg atgtcatgct ggccagtggc
     1561 acccaaagcg tggcggcgct gcaaagggct acccgcacct tgccgatcgt gttcgtgggc
     1621 gtcgccgatc cggtcggtgc cggcttgatc gacagtctgg cccgcccggg cggcaacacc
     1681 accggcttca tggagttcga gtacagtttc agcggcaaat ggctggagtt gctc
//
LOCUS       DATLJT010000078         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2989882,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000078 DATLJT010000000
VERSION     DATLJT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989882"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            978..1448
                     /locus_tag="VM910_01640"
     CDS             978..1448
                     /locus_tag="VM910_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVI61286.1"
                     /translation="MREADDRIVNDERRSGRSQGTRKDADNQTAVVSVNNAYLAAKNP
                     WLQRRFRAPIGLGDLPFVVGRGPVAGEGLPPLPPDLKLNDTVPFRLSRNHFMIEKRNG
                     SYYVRDLCSTLGTIVNGEPIGEHFRGDDAPLRAGENEVIAGGVDSPFVFSVLIG"
     gene            2128..2358
                     /locus_tag="VM910_01645"
     CDS             2128..2358
                     /locus_tag="VM910_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HVI61287.1"
                     /translation="MVERWVKLFKNGRNQAVRIPREFELPGEDAVIRKEGQRLIIEPA
                     PPKSLLAVLATLQTLEEEFPPIEGLPAEPVNL"
     gene            2361..2759
                     /locus_tag="VM910_01650"
     CDS             2361..2759
                     /locus_tag="VM910_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVI61288.1"
                     /translation="MGFLLDTNVLSDLVRRPHGRIADRIGQVGEQNVCTSIIVAAELR
                     YGAARKASPLLSAQLETVLGALEVLALESPVDVVYGELRTRLERAGRSIGANDLLIAA
                     HALALAHTVVTDNEREFSRIDDLRIENWLR"
     gene            complement(2880..>3165)
                     /locus_tag="VM910_01655"
     CDS             complement(2880..>3165)
                     /locus_tag="VM910_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI61289.1"
                     /translation="NLRAINVTPHVAQNDSITSTGKRRRSAIDGRTTRHQGYGTSQSC
                     RAMTECIFGWGKQHGTMRKTKHRGIAAVAADFLLNLIAYNLIRIPKLVAA"
BASE COUNT          635 a          885 c          969 g          676 t
ORIGIN      
        1 tcctgcacga tgttgaccgc gtaaaggcgc aacgagtcgt cggtggactg ggcctggctg
       61 gtaggctcgg catgcgcgta cccgagcagc atcaaggcca tcactgccaa gcagaattca
      121 atcgcacggc gcacggttga ccttcggccc aacgcgcatg aaagatgccc cacgcttgtc
      181 cctaaagata gggttccggg cccctgtgtc aaacgatgtt ccgatcaatg gccgaggcac
      241 acgatgcggc gttgggttag ttctgctaaa aaagttggat tgaatcatct ctggtaatcg
      301 ggctcggagc gcccaaaaca gccatctgac gacgagcaca gtcgttgttc gctgacaaga
      361 tttcagaaac ggcgcgacgc gacccaatta gtccgcgaca caagcagggg aatggatgca
      421 tgccggcgcg gcacgccacc cgccaccgac cggtgcgctt tcacgtacgt ggattcgatc
      481 atctcggtgt ccgtggatca tccgttccca gcaagttccc gaaacaggtt ttcccagacg
      541 ccgcgccgct cccaatcccc ttccaaaagc cactcaggat cgtactgtca gggtcgagaa
      601 attaatgagt tctgacccta gactagtttg ccctcattgg gtctgccacg attgagagga
      661 ccacaatgac tcgcacgcac ttcgccgaag ggcaaatcct cttcagagag ggagatccgg
      721 cggatagtgt ttttcgcctc ctcagcggtg ccgtcgacat actccgtgaa ctcgatggcg
      781 acccgatcct tctcgggacg gttgacgcag gacagcttat aggggagatg ggcgttgtgg
      841 aaaaccggcc ccgcagtgcg acggctcgag tcgccagcga ggtcgaggta gagatcctca
      901 cgccgaccga gttccttgat cagattgtag cctcgccgcg ggccgcccgc gagttgattc
      961 ggcgcctcag tcagcggttg cgcgaggccg acgatcgcat cgtcaacgat gaaaggcgaa
     1021 gcggccgatc ccaggggacc cgcaaggacg ccgacaacca aacggctgta gtgtcagtca
     1081 ataatgctta tctcgctgcc aagaatccgt ggttgcagcg tcggttccgc gctccaatcg
     1141 ggctcggcga tctgccgttc gtcgttggcc gggggcccgt tgcaggggaa gggctgccgc
     1201 cgttgccgcc agacctcaag ctcaatgaca ccgtcccgtt cagactatcg cgcaaccact
     1261 tcatgatcga gaagcgcaac ggaagttatt atgtgcgcga cctctgcagc acgcttggaa
     1321 cgatcgtcaa cggcgagccc atcggcgagc atttccgcgg tgatgatgcg ccgctgcgag
     1381 cgggagagaa tgaagtgatt gccggcggcg tggattcgcc gtttgtcttc tccgtgctca
     1441 taggttgaga ctgctggagc gttcgtgcgc gggatctcta atcgcccaag atcgaacttg
     1501 ctctgcatgg cgatctcctc cggtcggctc ttttgcgtca gcaccagcaa cgtctgcgca
     1561 attaaaggct cggctcagtc cgggccgagg atggtttaga gccgctccat gtacattgtc
     1621 gctgacgcgg ctgacggtgc tgatcctccc cggacagcgg tgcctcgtac ggcataccct
     1681 gaaggctttc cctcatcgag atgaattggc ttgatcagca ggccggtcag aggacacttt
     1741 cgggtggcca tgacgtgcct tttggctgat tcgaggaaaa aacattattg gcgtggtgtt
     1801 ggccacaatg agtttgagta tgcggcgttg gaccacctcg gtctcggcct ggggtcgccg
     1861 gagtgcattg caagtcttgc gccttgccta gtttgcaaag tctgtcgtca cgtcactcga
     1921 agctgatgta cgacagccaa gtcggccgac gcgagctcgg tgatcgacca catcctcggc
     1981 aggcgggtgc gctcacccta ttggaaagct gtgtgcagga ccggaggaaa ctgtgcttgt
     2041 cggagcctcc caacacggcc ttggcaactt ggccgtttcg caccgaccgg gcttgacata
     2101 tgccgaggca tatgccagtt aggattgatg gtcgagcggt gggtgaaatt attcaaaaat
     2161 gggcgcaatc aggccgtgcg gattccgcgc gagttcgagc tgccgggaga ggatgcagtt
     2221 atccgaaagg aaggccagcg acttattatt gaaccggcac cacccaaatc gctgctggcc
     2281 gttctcgcga ccctacagac gctcgaagag gaatttccgc caatcgaagg gcttccggca
     2341 gagcccgtga acctgtgata atgggtttcc tgctcgacac caatgtcctt tcggacctcg
     2401 tgcgacgtcc gcatggacgg attgccgatc gcattgggca ggtcggagaa cagaacgtct
     2461 gtaccagtat catcgtcgct gccgagttgc gctatggggc ggcgaggaaa gcctcgccgc
     2521 tgttgagtgc gcagctcgaa accgtgctgg gtgcactcga ggtgctcgcg ctcgaatcgc
     2581 cagttgatgt tgtttatggc gaactaagaa cacggctcga acgcgctggt cgatccattg
     2641 gcgcaaacga cttgctcatc gccgctcatg cattggcatt ggctcatacg gtagtcaccg
     2701 ataatgagcg agaattctcc cggatcgatg accttcggat cgaaaattgg ctgcggtaat
     2761 gcttgatggc aagacctagc agtttgcgga aaaaatttag aaactgaacc attgtcatct
     2821 cagatgaggg tcgagttttg acgatggatc gagctttggt cccttcgatc ggccgtggac
     2881 tacgccgcga ccagtttggg gatgcggatc agattgtagg cgatcaagtt gagcaggaaa
     2941 tcggccgcga cggcggcgat gccgcggtgc ttggtcttgc gcattgtacc gtgctgtttg
     3001 ccccagccga agatgcattc ggtcatcgct cggcaggatt gcgacgtgcc gtagccttgg
     3061 tgccgcgtgg tgcgtccgtc gatggcgctg cggcgacgct tgccggttga ggtgatgctg
     3121 tcgttctgtg ccacatgcgg cgtgacgttg atggcgcgta gattg
//
LOCUS       DATLJT010000079         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381718,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000079 DATLJT010000000
VERSION     DATLJT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381718"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VM910_01660"
     CDS             complement(<1..194)
                     /locus_tag="VM910_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61290.1"
                     /translation="MSVMIEMTGAEKTFTMHLQGGVRLPVVRGVTFQVKAGECVVLSG
                     PSGAGKSSILKMIFGNYRCDG"
     gene            complement(203..1003)
                     /gene="phnK"
                     /locus_tag="VM910_01665"
     CDS             complement(203..1003)
                     /gene="phnK"
                     /locus_tag="VM910_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HVI61291.1"
                     /translation="MADVQRETQCDQPLLVAEGLSKSYGRLAACRDVSFTLYPGEVLA
                     IVGESGSGKTTLLQMLSAQLASSAGHVSYRMRDGVLRDLNSLGEAERRFLFRTDWGYV
                     RQDPAQGLRMAFSAGANVGERLMAVGWSHYGRIRDAASSWLERVEIDTARIDDAPRTY
                     SGGMRQRLQIARNLVTEPRLVFMDEPTGGLDVSVQARLLDLMRNLVSELGLAAIVVTH
                     DLAVARLLSHRVMVMKGGEVIETGLTDQVLDDPHEPYTQLLVSSILPP"
     gene            complement(996..1880)
                     /locus_tag="VM910_01670"
     CDS             complement(996..1880)
                     /locus_tag="VM910_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HVI61292.1"
                     /translation="MNAPAYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFASREMPMP
                     YGWGTGGVQVTAAILGPDDVLKVIDQGSDDTTNAISIRKFFAKTAGVATTTATTEATV
                     IQTRHRIPEAPLHAGQVLVYQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDI
                     ARFGYIATSYAYPVKVNARYVMDPSPTPKFDNPKMGNCPALQLFGAGREKRIYAIPPH
                     TTVVSLDFEDHPFTPYRFDAPCALCGAGDAYLDEIVTDDNGSRMFVCSDTDYCESRQA
                     AGHRGSESAAPHKERAHG"
     gene            complement(1877..>2536)
                     /locus_tag="VM910_01675"
     CDS             complement(1877..>2536)
                     /locus_tag="VM910_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HVI61293.1"
                     /translation="AATPRVTDILGRDGLIEPSPQQDADTPVGDLTREPLNFPAERDL
                     RLQNLARADEGFLLAMGYSTQRGYGRNHPFAGEIRFGEVEVEFFAEDAGFAVPLGAIA
                     LTECQMVNQFKGSATEPPCFTRGYGLAFGQSERKTMSMALVDRSLRARELGEEVLAPA
                     QDEEFVMSHSDNVQATGFVEHLKLPHYVDFQSELGLLRKLRKEFAESADRAEPLQEAA
                     E"
BASE COUNT          473 a          818 c          796 g          449 t
ORIGIN      
        1 ccgtcgcagc gataattgcc gaaaatcatc ttcaggatcg acgacttccc ggcccccgac
       61 ggcccggaca gcaccacgca ctcgccggcc ttgacctgga aggtcacgcc gcggacgacc
      121 ggcaacctta ctccgccctg caaatgcatg gtgaaggtct tttcggcccc ggtcatttct
      181 atcattacgg acattggcgt gctcatggcg gcaggatcga tgagacgagc agttgcgtat
      241 aaggttcatg cggatcgtct agcacctggt cggtcaaccc ggtctcgatc acctcgccgc
      301 ccttcatcac catcacccgg tgcgatagca gccgcgcgac cgcgagatcg tgcgtcacca
      361 caatggccgc caatcccaac tcgctgacga ggttacgcat taaatcgagg agccgcgcct
      421 gcaccgagac gtcgagcccg cccgtcggtt catccatgaa gaccagccgc ggctcggtaa
      481 cgagattgcg cgcgatctgc aggcgctggc gcatgccgcc cgaataggtc cgcggcgcat
      541 cgtcgatccg cgccgtatcg atctcgaccc gctccagcca ggacgaggcc gcatcgcgga
      601 tgcggccata gtgactccag ccgactgcca tcagccgctc gccgacattg gcgccggcgg
      661 agaaggccat gcggaggcct tgcgccggat cctggcgcac gtagccccag tcggtgcgga
      721 acagaaagcg ccgctccgct tcgccgagcg aattgagatc gcgcaagacg ccgtcgcgca
      781 tgcggtaaga cacgtgtcct gcgctggacg ctagctgagc ggagagcatt tgcaagagcg
      841 tcgtcttgcc ggagccggat tcgccgacga tcgccagcac ctcgccggga tagagcgtaa
      901 aggacacgtc gcgacaagcc gcaagccggc cgtagctctt ggacaggcct tccgcaacca
      961 ggagcggctg gtcgcactgt gtctcgcgtt gcacgtcagc catgcgccct ctccttgtgc
     1021 ggagctgcgc tctcactgcc gcgatgaccc gccgcctggc ggctttcgca gtaatcggtg
     1081 tcggagcaga cgaacatccg gctgccattg tcgtcggtga cgatctcgtc gagataggca
     1141 tcgccggcgc cgcacagcgc gcaaggcgcg tcgaagcggt acggcgtgaa cggatgatct
     1201 tcgaaatcga gcgataccac ggtggtgtgc gggggaatcg catagatgcg cttttcgcgg
     1261 ccggcgccga acaattgcaa cgcagggcaa ttgcccatct tcgggttgtc gaatttcgga
     1321 gtcggcgacg ggtccatcac atagcgcgca ttgaccttca ccgggtaggc ataggaggtc
     1381 gcgatataac cgaagcgcgc gatgtcttcg tagagcttga catgcattag gccgtattca
     1441 gcaagcgcat gcatgcgccg cgtttcggtc tcgcgcggct cgaggaagcg taggggttcc
     1501 gggatcggca cctgatagac cagcacctgc cctgcgtgca gcggcgcctc cggaatccgg
     1561 tgacgggtct ggatcacggt cgcctccgtc gtagcggtcg ttgtcgccac gcccgcggtc
     1621 ttggcaaaga atttccggat cgagatggcg ttggtggtgt catcggagcc ctggtcgatc
     1681 accttcagca catcgtcagg cccgaggatc gcggcggtga cctgcacgcc gccggtgccc
     1741 cagccatagg gcatcggcat ttcacggctg gcaaacggca cttgataacc cgggatcgcg
     1801 acggccttga ggatggcgcg gcggatcatc cgtttggtct gctcgtcgag ataggcgaaa
     1861 ttgtaggccg gggcgttcat tcggcggcct cctgcagggg ctccgcgcgg tcggcgcttt
     1921 ccgcaaattc cttgcgcagc ttacggagca gtccgagctc ggactggaag tcgacataat
     1981 gcggcaactt caggtgttcg acaaaaccgg tcgcctgaac attgtcggag tgcgacatca
     2041 cgaactcttc gtcctgcgcg ggcgccagga cctcctcgcc gagctcgcga gcacgcaagc
     2101 tccggtcgac cagcgccatc gacatcgtct tgcgctcgct ctgtccgaaa gcgagaccat
     2161 aaccacgagt gaagcacggc ggctccgtcg ccgatccctt gaactgattg accatctggc
     2221 attcggtcag cgcgatcgcg ccgagcggca ccgcgaagcc ggcatcctcg gcaaaaaact
     2281 caacctcgac ctcgccaaag cggatttcgc cggcaaaggg atgattgcgg ccatagccgc
     2341 gttgggtcga atagcccatc gccagcagaa agccttcgtc ggcgcgcgcg aggttctgca
     2401 gacgcaaatc gcgctcggcc ggaaaattca gcggctcgcg cgtgaggtcg ccgaccggcg
     2461 tatccgcatc ctgctgcggc gacggttcga tcaggccgtc acggccaaga atatccgtga
     2521 cgcgcggagt ggccgc
//
LOCUS       DATLJT010000080         7271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_462716,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000080 DATLJT010000000
VERSION     DATLJT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462716"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VM910_01680"
     CDS             complement(<1..708)
                     /locus_tag="VM910_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61294.1"
                     /translation="MSRHAVNGAAVTALCFLCSLLAPAASAAPPQRGGAPAAMPHAAA
                     PAPHFAAPAPHFAAPAPRMAAPRVAAPAPHFSAPARVAAPHTAPPRNFAARPASPHFA
                     ETPRGQAPQARAQGFAGSSRHATIAHVNRELNRPGAAAPSEAARHATENAKTGPEGRI
                     RQQPVTGPGQTANLNRQNRGATEAERTRLDQGRFAQARRSPIIKNPVFASQSPRAVGT
                     SLSNWTFRGHFAQSAFFR"
     gene            1082..1234
                     /locus_tag="VM910_01685"
     CDS             1082..1234
                     /locus_tag="VM910_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61295.1"
                     /translation="MTKTQAEYRDTPNGIYSCATCTLFDKPNTCKVVEGEVSPDGWCR
                     AFAMAD"
     gene            complement(1290..2732)
                     /locus_tag="VM910_01690"
     CDS             complement(1290..2732)
                     /locus_tag="VM910_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HVI61296.1"
                     /translation="MPADSGKKLFVGPRFRRIRQQLGLSQTQIAEGLGISPSYINLIE
                     RNQRPVTAQILLRLAEAYDLDLRDLATADEDRFFAELNEVFSDPLFRQIELPKQELRD
                     LAELCPGVTHALQRLYAAYTEARRGETLVAAQMADREGSQFEANPIERVRELIEANRN
                     YFAELEQAAEQLRDELNVPAEGLYTALAARLRETHSIVTRIMPVDVMRETLRRFDRHR
                     RQLLISELVDGPGRTFQLAFQLGLAECAPQLETILGRAGALDDAPRRLYRITLANYFA
                     ASVMMPYQPFLTAAEALSYDVHVLAQRFNAGFEQVCHRLTTLSRPNARGIPFFLLRVD
                     NAGNVSKRFSSGTFPFSKFGGTCPLWNVHSTFDTPDRLLKQVIELPDGTRYFSIAQMV
                     RRPTVPHPLPQPRFAIGLGCEIRHASKLVYAAGMDLEKTEGTPIGVNCRLCERENCSQ
                     RAEPPITRTLILDENTRRVSSFAFSNAREL"
     gene            2911..4221
                     /gene="aceA"
                     /locus_tag="VM910_01695"
     CDS             2911..4221
                     /gene="aceA"
                     /locus_tag="VM910_01695"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVI61297.1"
                     /translation="MAKTFEQLVPNAPEGRFEGISRPYGPAEVERLRGSVPIAYTLAE
                     RGANRLWKALSNEPYVNSLGAVTGNQAMQQARAGLPAIYLSGWQVAADANTAGAMYPD
                     QSLYPANAGPELCRRINRTFQRADQIEHSEGGAKIDWFVPIVADAEAGFGGPLNAFEI
                     MKAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTAAHERNLIAARLAADVCGVPSFV
                     LARTDAESAKLITSDVDERDREFLTGERTAEGFYRLKPGTGLAHCIKRGLAFAKYADL
                     LWWETSTPNLDEAKAFAEAVRKVYPNKMLAYNCSPSFNWEANIDKATIAKFQREIGAM
                     GYKFQFVTLAGFHSLNHGMFELARGYKAEGMAAYSRLQQAEFASEKFGYSATRHQREV
                     GTGYFDLVSTTISGGQSSTTALHDSTEAAQFKSHGSDIRVQAAE"
     gene            4234..4443
                     /locus_tag="VM910_01700"
     CDS             4234..4443
                     /locus_tag="VM910_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61298.1"
                     /translation="MTKGSNFWVIGGEFGSMNFHKLVEGSAQVKGPFKTRKEAEDCWR
                     EVSEENRHKAGVRFSIVEEPNRSAA"
     gene            4670..5227
                     /locus_tag="VM910_01705"
     CDS             4670..5227
                     /locus_tag="VM910_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61299.1"
                     /translation="MSEAQTTGNSGAGEQRPIRLRDALRQARIEAADRTGVVVDLRDA
                     ELARLEILNEALDPLFAQVPDTVNLFDRGISQGETPRLWIDVVAHVVMGRDKRIYRFV
                     QDTRFGRKVLAESHEVPAIVGAVTDYVARRIVEREQALVATPEQGSVKRKKRRGGGFW
                     TFVLGFVLGAAALFGLALFAALRSL"
     gene            complement(5233..6120)
                     /locus_tag="VM910_01710"
     CDS             complement(5233..6120)
                     /locus_tag="VM910_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI61300.1"
                     /translation="MAAFTLAHLSDPHLPPLPAPRLSELVGKRALGYLNWTRNRRKYH
                     RREVLDALVADLASQSPDHIAVTGDLVNLALESEFPLALKWLESVGPPERVTAIPGNH
                     DAYVRATRDRFAQSWAGYLRDEQAGGHVTFPSLRRRGPMALISLSSAVPTPPLMATGT
                     LGRTQLAALERVLKTLAAEQTFRVLLVHHPLRSDSRIKRLTDSAELIALIRLHGVELV
                     LHGHDHVHSTVWLEGPNGAIPVIGVPSASALAHGRHPAAAYNLYSIEHDNAVWRCEQI
                     VRGIDAKSGLREIGRVRLF"
     gene            6257..6742
                     /locus_tag="VM910_01715"
     CDS             6257..6742
                     /locus_tag="VM910_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI61301.1"
                     /translation="MSGPTLRKRLEPALRRLFHLYWRVARGMTLGVRGVVLDRDNKVF
                     LIKHSYVSGWHLPGGGVEIGENLVDALKRELFEEGRIELAGKPELHGVFFNSHVSTRD
                     HVAVFVVREFRQDRPPEPNHEIVATGFFPPAALPEGTTEGTRRRIGEVLDGVAQIATW
                     R"
     gene            6733..>7271
                     /locus_tag="VM910_01720"
     CDS             6733..>7271
                     /locus_tag="VM910_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61302.1"
                     /translation="MALGLAWTRSWSDKMNPLKAICGLLCLLVLASNLWSMSRWNEAR
                     GVFDDVCYLRQAHLFQRFGLAGFDTDISRDDDHYLTSKLKEIGFPTWNDVATAPCHNP
                     MPALGRFVIQYPPGTGLVMAMFPSGHQVVPLYVLATIAVFGFALLGIVAARSTAATLL
                     AGAFGCLAIYLMINPSKAS"
BASE COUNT         1353 a         2169 c         2432 g         1317 t
ORIGIN      
        1 gcggaagaac gccgattgcg caaaatgacc gcggaaggtc cagttcgaaa ggctcgtccc
       61 cactgcacgc ggcgactgcg aggcgaagac cgggttcttg atgatcggcg acctccgcgc
      121 ttgcgcaaac cggccctgat ctagacgcgt gcgctcagct tcagtcgcgc cccggttctg
      181 acggttgaga ttggcagtct ggccgggacc ggtcacgggc tgctgccgga tgcggccctc
      241 ggggccggtc tttgcgttct ccgtcgcgtg tctggccgct tcagacggcg cagccgcacc
      301 gggccggttc agctcgcggt tgacgtgcgc aatcgttgca tggcgcgacg agccggcaaa
      361 tccttgcgct cgcgcctgcg gtgcctgccc cctcggcgtc tcggcgaagt gcggcgaagc
      421 cgggcgcgcc gcaaaatttc gcggcggcgc cgtatgtggt gcggcaacgc gcgcaggcgc
      481 ggagaaatga ggggcaggtg cggcgacgcg cggtgccgcc atacgaggtg cgggtgccgc
      541 gaaatgcggt gcaggtgctg cgaagtgcgg tgcaggtgcg gccgcgtgag gcatggcggc
      601 aggcgcgccg cctcgctgcg gtggcgccgc cgaagctgcg ggagccaaga gtgagcagag
      661 gaagcaaagc gcagtcaccg ctgcgccgtt caccgcatgc cgcgacatgg ccgtaactcc
      721 gaaaaaatca cgtcaaattt caaacgcgac gtgcgcaatt ttgttcctgc taggctgaat
      781 gtgaactgaa cgcgcgcact cgactttcgc agcaattttt cgcagcgatt ggagaatgtg
      841 aacagttgtt caggcaatcc gacgcgcaat cctggcggaa cgacacgctg atgcgacctc
      901 ggtcgggtgc gagcatgacg gcggatgatt gcgctgctac agtgaggaca aggagaagaa
      961 gccgagagaa ggacgcatgg tcgaacaacg caactgctgg acaaggcgac aggcgattgg
     1021 cgcggttgca ggcggtgttt catcggcgct ttggcttcgg tcctcccgtg catcggagaa
     1081 gatgaccaag acgcaggccg aatatcggga cacacccaac ggcatctata gctgtgcaac
     1141 ctgcacgctg ttcgacaaac ccaacacctg caaggtggtc gaaggcgagg tcagcccgga
     1201 cggctggtgc agggcctttg cgatggcgga ttagaagccg cattttttgt cgtaccggca
     1261 aagcgagcgc cgagtcggga cgacgacgat cacaactccc ttgcattcga gaacgcgaag
     1321 gacgacaccc gccgcgtgtt ctcgtcgagg atcagcgtgc gggtgatcgg cggctcggcg
     1381 cgctggctgc aattctcgcg ctcacagagc cggcaattga cgccgatggg tgttccttcg
     1441 gttttttcga gatccatgcc tgcggcatag acgagttttg aagcgtgacg aatctcgcag
     1501 ccgagcccga tcgcaaagcg cggttgcggc aaagggtgcg gcaccgtcgg ccgccgcacc
     1561 atctgcgcga tcgagaaata gcgcgtgccg tccggcagtt cgatcacctg cttgaggagc
     1621 cggtccggcg tgtcgaaggt cgaatgcaca ttccacagcg ggcaggtgcc tccaaatttg
     1681 gagaacggga aggtgcccga ggagaagcgc ttggagacgt tgccggcatt gtcgacgcgc
     1741 aacaggaaga agggaatgcc gcgcgcgttc ggccgcgaga gcgtggtgag gcgatggcag
     1801 acctgctcga atcccgcatt gaaccgctgc gctaacacat gcacgtcgta gctcagcgct
     1861 tcggccgcag tgagaaaagg ctgatacggc atcatgacgc ttgccgcaaa atagttcgca
     1921 agcgtgatgc ggtagagccg ccgcggagca tcgtcgagcg cgccggcgcg gcccaggatg
     1981 gtctcgagtt gcggggcgca ttcggcgagc ccgagttgga acgcgagctg aaaggtgcgt
     2041 cccggtccgt ccaccagttc ggagatcaaa agctgccggc gatggcggtc gaagcggcgc
     2101 aatgtctcgc gcatgacgtc gaccggcatg atgcgggtga cgatggaatg tgtctcgcgc
     2161 aggcgggccg ccagcgcggt atagagtcct tcggccggta cgttgagctc gtcgcgcaat
     2221 tgttctgcag cctgctcgag ctcggcaaaa taattgcggt tggcctcgat cagctcgcgc
     2281 acgcgctcga tcggattggc ctcgaactgg ctgccttcgc ggtcggccat ttgcgctgcg
     2341 accagcgtct cgccgcgccg cgcctcggtg taggcggcgt aaaggcgctg cagtgcatgg
     2401 gtgacgcctg ggcagagctc ggcgagatcg cgcaattcct gtttcggcag ttcgatctgg
     2461 cggaacagcg gatcggaaaa gacctcgttg agttccgcaa agaagcggtc ctcgtcggcg
     2521 gtggcaagat cccgcaggtc gaggtcatag gcctccgcca gccgcaagag aatctgcgca
     2581 gtcaccggac gttggttgcg ctcgatcaag ttgatgtagc ttggggaaat gccaagtccc
     2641 tcggcaatct gggtctgaga aaggccgagt tgctggcgga tgcgccgaaa cctcgggcca
     2701 acgaacagct tctttccgga atcggcgggc atggcagctt ggctgaccta tattgtgaca
     2761 aactttacag aataacatat attacaagtg catatgttac atgacatcac cattcgaatg
     2821 caagccgtct gtacgaacta aaaagtttcg cgtttaggta ccgccacgca tttcgctttg
     2881 cactgtcaaa aatgtcgatt ggagagaacg atggcgaaga cctttgaaca actcgtcccc
     2941 aacgcgccgg aaggccgttt cgagggcatt tcccgcccct atggcccggc cgaggtggag
     3001 cggctgcgcg gctccgtgcc gatcgcctac acgctcgccg agcgcggcgc caatcggctg
     3061 tggaaggccc tgagcaacga gccctatgtc aactcgcttg gcgcggtcac gggtaaccag
     3121 gcgatgcagc aggcacgcgc tggcttgccc gcaatctatc tgtccggctg gcaggttgcg
     3181 gccgacgcca acaccgcggg cgcgatgtat ccggaccaga gcctttatcc ggccaacgca
     3241 ggccccgagc tgtgccgccg catcaaccgc acgttccagc gtgccgacca gatcgagcac
     3301 tccgagggcg gcgccaagat cgattggttc gtgccgatcg ttgccgacgc cgaagccggc
     3361 ttcggtggtc cgctcaacgc gttcgagatc atgaaggcct atatcgaggc gggcgcggcc
     3421 ggcgttcact tcgaggacca gctcgcgtcc gaaaagaagt gcggtcacat gggcggcaag
     3481 gtgctgatcc cgaccgcggc gcatgagcgc aacctgatcg cggcgcggtt ggctgccgac
     3541 gtttgcggcg tgcccagttt cgtgctcgcc cgcaccgacg cggagagcgc caagctcatc
     3601 acctcggatg tcgacgagcg cgaccgcgag ttcctcacgg gcgagcgcac ggcggaaggc
     3661 ttctaccggc tgaagccggg aacgggcctc gcgcactgca tcaagcgcgg ccttgccttt
     3721 gcaaagtacg cggaccttct gtggtgggaa acctcgacgc cgaacctcga cgaggcgaag
     3781 gcctttgcgg aggccgtgcg caaggtctat ccgaacaaga tgctggccta taactgctca
     3841 ccctctttca actgggaggc caacatcgac aaggcgacca tcgcgaaatt ccagcgcgag
     3901 atcggcgcga tgggctacaa gttccagttc gtgacgctgg cgggcttcca ctcgctcaat
     3961 cacggcatgt tcgagttggc gcgcggctac aaggccgaag gcatggcggc ttactcccgc
     4021 ctgcagcagg ccgagttcgc gtcggaaaaa ttcggctact cggccacgcg ccaccagcgc
     4081 gaggtcggca ccggctactt cgacctggtc tcgaccacga tctccggcgg tcagtcgtcg
     4141 accaccgcgc tccacgactc caccgaagcc gcgcagttca agtcgcacgg ttcggacatc
     4201 cgagttcagg ctgctgaata acaggaggat tttatgacca aaggcagcaa tttctgggtg
     4261 attggcggtg agttcggctc gatgaacttc cacaagctcg tggaaggctc ggcccaggtc
     4321 aaaggaccct tcaagacgcg caaggaagcg gaagattgct ggcgcgaggt ctcggaagag
     4381 aatcgtcaca aggccggcgt gcgcttctcg atcgtcgagg agcctaaccg ctccgcagct
     4441 tgatggagtg ttgccgggta tgcgccgcat gatccgatcc cggtctcgga tcggatcgtg
     4501 tgcgacacgt gcccagagga tggcggcctc atccggtttg atccgggtga ggccgtccgt
     4561 gttttcagaa cagtcgcata taagtcattg agagcaaacg acctttgcgg actgcgcggt
     4621 tactgattag gttaatcggg caaacacccg tcacgacaag ggctcttcaa tgtcagaggc
     4681 gcagaccacc ggaaatagcg gcgccggcga gcagcgtccg atccgcttgc gcgatgcgct
     4741 tcggcaggcg cggatcgagg ccgccgaccg caccggtgtc gtcgtcgacc tgcgcgatgc
     4801 ggagcttgct cggctcgaaa ttctcaacga ggcgctcgat ccgttgtttg cgcaggtgcc
     4861 ggacaccgtc aacctgttcg atcgcggtat cagccagggc gaaaccccgc ggctgtggat
     4921 cgacgtcgtc gctcatgtgg tgatggggcg cgacaagcga atttatcgct tcgtgcagga
     4981 cacgcgtttc gggcgcaagg tgcttgcgga atcccatgag gtcccggcga tcgtcggtgc
     5041 ggtgaccgat tacgtcgcgc gccgtattgt ggaacgcgag caggccctcg tcgcgacgcc
     5101 ggaacagggg tccgtcaagc gcaaaaagcg ccgcggtggc gggttctgga ccttcgtgct
     5161 cggctttgtc ctgggcgccg cagcgctgtt cgggcttgcg ctgttcgcgg cgttgagaag
     5221 tctctaaagc cgtcagaaca acctgaccct gccgatctcc cgcaggcccg atttcgcatc
     5281 gatcccgcgt acgatctgct cgcagcgcca gaccgcattg tcgtgttcga tcgaatagag
     5341 attgtaggct gcggccggat ggcggccgtg agcgagcgcc gaggccgacg gtacgccgat
     5401 cacggggatt gcgccgttcg ggccttcgag ccagaccgtg gagtggacat gatcgtggcc
     5461 gtgcaggacg agctctacgc cgtgcagtct gatcagcgcg atcagctcgg ctgaatcggt
     5521 caggcgcttg atccgcgaat ccgagcgcaa ggggtgatgc accagcaata cgcgaaatgt
     5581 ctgctcggcg gccagcgtct tcaggacgcg ctccagggcg gcgagctggg tgcggccgag
     5641 cgttccggtc gccatgagag gcggcgtcgg cacggccgag gacaggctga tcagcgccat
     5701 cgggccgcgg cggcggagcg atggaaacgt cacgtgaccg ccggcctgct cgtcgcggag
     5761 atagccggcc cagctctgcg caaaacgatc gcgcgtcgcg cgcacatagg catcgtgatt
     5821 gccgggaatg gcggtgaccc gttccggtgg gccgacgctt tcgagccatt tgagcgcgag
     5881 cgggaattca ctttcaaggg cgaggttgac gagatcgccg gttacggcga tgtggtcggg
     5941 cgactgcgag gcaaggtcgg ccacaagcgc gtcgagcact tcgcgccgat ggtatttgcg
     6001 gcgattgcgg gtccagttga gatagccgag cgcgcgcttg cccacgagtt cggaaagccg
     6061 cggcgcgggc agcggcggca gatggggatc ggacaggtgc gcgagcgtga aggcggccat
     6121 tatgcccgtc cctgcgcctt cccggaactg tcggaccgct gctcggtcac ccctggtatc
     6181 ctcccgccgt cacttcatct attggcaagg ggcccgcgcg agcgcaagga tcaaacagga
     6241 tggaaatttg gttgaaatga gcggccccac tttgcgaaag cgccttgaac cggcattgcg
     6301 gcgcttgttt cacctttact ggcgcgtcgc ccgcggcatg acgcttggcg tccgcggcgt
     6361 cgtgctggac cgggacaaca aggtgtttct gatcaagcac agctatgtct ccggctggca
     6421 tttgccgggc ggcggggtcg aaattggcga aaaccttgtc gacgcgctca agcgcgagct
     6481 gttcgaagag gggaggatcg agctcgccgg caagccggag ctgcatggcg tcttcttcaa
     6541 cagtcatgta tcgacgcgag accacgtcgc ggtgttcgtc gtcagggagt tccggcagga
     6601 tcggccgccc gagcccaacc acgagatcgt ggccaccggc ttctttccgc ccgcagcact
     6661 gccggagggg acgaccgagg gcacacggcg acgcatcggg gaagtgctcg acggggtcgc
     6721 gcagatcgcg acatggcgct agggcttgcc tggacgagga gctggtcgga caagatgaat
     6781 cccctgaaag ccatctgcgg cctgctttgc ctgctggttc tcgccagcaa tctgtggtcg
     6841 atgtcgcgct ggaacgaggc gcgcggcgtc ttcgacgacg tctgctactt gcggcaggcc
     6901 cacctgttcc agcggttcgg attggccggc ttcgataccg acatttcccg cgacgacgat
     6961 cattatctga cgtcgaaatt gaaggagatc ggcttcccga cctggaacga cgtggcgacc
     7021 gcgccctgtc acaatccgat gccggcgctg ggaagattcg tgatccagta tccgcccggc
     7081 acaggtcttg tgatggcgat gtttccaagc ggacaccagg tggtgccgct ctatgtgctg
     7141 gcgacgatcg ccgtgttcgg ctttgcgctg ctcgggatcg tcgcggcgcg atcgacggct
     7201 gcaaccctgc tcgccggcgc attcggttgc cttgcgatct atctgatgat caatccgtcg
     7261 aaggcaagct a
//
LOCUS       DATLJT010000081         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4368519,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000081 DATLJT010000000
VERSION     DATLJT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368519"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VM910_01725"
     CDS             complement(<1..398)
                     /locus_tag="VM910_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI61303.1"
                     /translation="MIVTEQTPPRQRAREPRIDIDIATTRPGTPGGIFMRQFWHAVCR
                     SRDLPVGSAKPLRIMSEDFAIYRTQAGVPQVVAYRCPHRGAPLHLGWVEDEAIRCVYH
                     GWKFDHCGQCVEQPAEEAGFARKVTLKTYPT"
     gene            532..1770
                     /locus_tag="VM910_01730"
     CDS             532..1770
                     /locus_tag="VM910_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61304.1"
                     /translation="MTGIFASRWWIVFACACGLLVGAGAILIFTFGVFLKPVTEDLGV
                     TRGELSGALGVSTWFVAVSCPVIGWLIDRFGTRHVMIPGILLFALAVASFGLMQAQPL
                     LLIFVIFCVAGFISGVQTPIPYAAVITHWFDSHRGIALGLATAGVGLGVAIIPQLAAL
                     LIGHFGWRWAYAGLGIAVVVLAWLPVLLFVREPPWVPSSGSADAASAAALPGIDAAAA
                     LTTRNFWLLSVAFFLGVLAINGTIVHIVALLTDRGVPLQTATGALSVAGIAIIVGRII
                     CGWCLDRFWGPYVAVCFFVLPMLGIALLGSGWGFPVPLVGAALCGAGIGAEIDLMAFF
                     ISRYFGLKAYGRIYGLMFMLFNIGTGMGPALSGRAYDRFHSYSQIFTVYEVALALTCV
                     LLVGLGKYAYPPVKHAPQPA"
     gene            1929..>2528
                     /locus_tag="VM910_01735"
     CDS             1929..>2528
                     /locus_tag="VM910_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61305.1"
                     /translation="MTYVPMQPPILSLAERDRRWARVRSLMRERRFDGLLVAGFRSRE
                     MYETYISDDYNEGCVIFPLEGDPVVITWAHLRVMRARWSQERGTKLWVDDYRVATNGT
                     AVAEVLREKRLAQSSLGVVGLSSQAPTEIYGAIPANFWMELTAALPQARWEDISEEFS
                     HLMLVKSDEELAQVRYAARAAEAGCRMLVEVATPGIGEEV"
BASE COUNT          379 a          786 c          844 g          519 t
ORIGIN      
        1 gtcggatagg tcttcagggt caccttgcgg gcgaaccctg cttcctcggc cggctgctcg
       61 acgcactggc cgcaatggtc aaatttccag ccgtgataga cgcagcggat cgcctcatcc
      121 tcgacccagc cgagatgcag cggtgcgccg cgatgcgggc agcgataggc gacgacctgc
      181 ggcacgcccg cttgcgtgcg gtagatcgcg aagtcctcgc tcatgatgcg cagcggcttg
      241 gcgctgccca ccggcaggtc gcggctgcgg cagaccgcat gccagaactg acgcatgaaa
      301 atgccgcccg gcgttcccgg ccgcgtggtc gcgatatcga tgtcgatgcg cggttcgcgg
      361 gcgcgctgac gcggcggtgt ctgctcagtg actatcatga gcgtgctcct cttgaaccat
      421 gtttgcgcga ttatagcatc gtgccgcctg ggcgtggctt gtgggcgccg tgccgggaaa
      481 ctaacgttgc gaacaagcaa accgtccgag tgtctgagcg gggaaggcgt catgaccggg
      541 attttcgcca gccgctggtg gatcgtgttc gcttgcgcct gcggcctctt ggtcggcgct
      601 ggcgcgatcc tgatcttcac cttcggcgtg tttctcaagc cggtaaccga ggatctcggc
      661 gtcacccgcg gagaactgtc gggtgcgttg ggagtgagca cctggttcgt tgccgtgtcg
      721 tgcccggtga tcggctggct gatcgatcgt ttcggcacgc gccacgtaat gatccccggc
      781 attttgctgt tcgcgcttgc ggtcgcgagt tttggcttga tgcaggcaca gccgctcctg
      841 ctgattttcg tgatcttctg cgtggctggc ttcatcagcg gcgtgcagac gccgattccc
      901 tacgccgcgg tgatcacgca ctggttcgac agccatcgcg gcatcgcgct cggtttggca
      961 acggcgggcg tcggactggg cgtggccatc atcccgcagc tcgccgcgct tctgatcggc
     1021 catttcggtt ggcgttgggc ctatgccggg ctcggcatcg ccgtcgtggt gctagcctgg
     1081 cttcccgttc tcctgttcgt acgcgaaccg ccctgggtgc cgtcgtcggg cagcgccgac
     1141 gccgcgagcg cagcggcgtt gcccggcatc gacgccgcgg cggcgctgac aacgagaaac
     1201 ttctggctgc tgtcggtcgc gttctttctc ggcgttctag cgatcaacgg caccatcgtt
     1261 catatcgtcg cgctcctcac cgatcgcggc gtgccgctgc agaccgcaac cggcgcgctc
     1321 tcggtcgctg gcatcgccat catcgtcggc cgaatcatct gcggctggtg tctcgatcgg
     1381 ttctggggtc cgtatgtcgc cgtctgcttc ttcgtgctgc cgatgctcgg gatcgcgctg
     1441 ctcggcagcg gatggggatt tcccgttccc ttggtgggcg cggcgttgtg cggcgccggc
     1501 atcggcgccg aaattgatct catggccttc ttcatcagcc gctatttcgg gctcaaggcc
     1561 tacggccgga tctatggcct gatgttcatg ctgttcaaca tcggcaccgg catgggcccg
     1621 gcgctgagcg ggcgcgccta cgaccggttc cactcctatt cgcagatctt cacggtctat
     1681 gaggtcgccc ttgccctgac ctgtgtgctc ctggtcgggc tcggcaaata tgcctatccg
     1741 ccggtcaagc acgcgccgca gccggcgtga agtagatcgc gtgtagccct cgctccaaca
     1801 gagggacaag ctcggccatg acggtgcaca atagccctgc gcaaagagag ggaagcagct
     1861 cggtcgcgtg gcgcatgtgc agcaatcgca tgcgagtgca ggggccgaga tgaacgaaag
     1921 gagacgtcat gacctatgtc ccaatgcagc caccaatcct gtcgttggct gagcgcgatc
     1981 ggcgctgggc gcgagtccgc agtttgatgc gcgagcgcag attcgatggg cttctggtcg
     2041 caggatttcg gtcgcgtgag atgtacgaga cctacatcag tgacgattac aacgaaggct
     2101 gcgtcatttt cccgctcgag ggcgatccgg tcgtcatcac ctgggcgcac ttacgggtga
     2161 tgcgcgcccg ctggtcgcag gaacgcggca ccaagctctg ggtcgacgac tatcgcgtcg
     2221 cgaccaacgg caccgcagta gcggaagtgc tgcgcgagaa acgtctcgcc cagagcagtc
     2281 tgggggtggt tggtctcagc tcgcaggcgc ctaccgagat ctacggcgcg atccccgcca
     2341 atttctggat ggagctgacc gccgcgctgc cccaggcgcg ctgggaggat atctccgagg
     2401 agttcagcca cttgatgctg gtgaagagcg acgaggagct ggcgcaggtc cgctatgctg
     2461 cacgcgcggc cgaggcgggt tgccggatgc tcgtcgaggt ggcaacgccc ggtatcggag
     2521 aggaggtc
//
LOCUS       DATLJT010000082         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3449552,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000082 DATLJT010000000
VERSION     DATLJT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3449552"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VM910_01740"
     CDS             complement(<1..536)
                     /locus_tag="VM910_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61306.1"
                     /translation="MRRREFITLFAGAAATAPIIAHAQTYPSRTITLVVPFPPGGVAD
                     YAARPLAAHLADTLGQRVVVENKGGAGGGIGHAYVARAEPDGYTIMTALPSLAVIPEG
                     NRLAGKPVPYEMDQFVPLARMFADPPILAVKNSSPWNSLEDFIAEVKAKPGQIPYGTS
                     GHLGTVHLAMEMFLNAAQL"
     gene            complement(637..1242)
                     /locus_tag="VM910_01745"
     CDS             complement(637..1242)
                     /locus_tag="VM910_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61307.1"
                     /translation="MLTSRLLGIFAVVSALVSPGAEFAVARDAPANDFSSLVEWKNQI
                     AADIQHNKRYLPGVRTRGKIGVVYFDLNINRAGWVLPGTRILTVDRELGSAALLLLQQ
                     SQPLPAPDHVGLRDATFRITVPVRFNEIPPQQAAGLQDLERRKLLECYEADREELEYQ
                     RRKAAYRKAEPPTEVYRMPEGCLEAKRQGAERERREADALR"
     gene            complement(1532..>2066)
                     /locus_tag="VM910_01750"
     CDS             complement(1532..>2066)
                     /locus_tag="VM910_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61308.1"
                     /translation="NRGGARVSKTSIAAVLLALGVLSLQEGTNAMVVPPSGLQPASAT
                     PAIAETVALRKDRTVTRHVVVRHRGSHVRTRHVASGHRRVAVRTRHVAVRHRAVATRR
                     THVAVAKRSAVAAKSTEVVRPVRPWVRQSYYGSVFDGVTLGAIIAASAVPTSPSFDVC
                     WYWSNSSQTRGYWDFCQ"
BASE COUNT          375 a          643 c          656 g          392 t
ORIGIN      
        1 agctgcgcgg cattgagaaa catttccatc gccaggtgca cggtgcccag atgcccggac
       61 gtgccatagg gtatctggcc gggcttcgcc ttgacctccg cgatgaagtc ttccaacgag
      121 ttccacggcg atgagttctt cactgccagg atcggcggat cggcaaacat gcgcgcgagc
      181 ggcacgaact ggtccatctc gtagggaacg ggcttgcccg ccagccggtt gccttcggga
      241 atgacggcaa gcgaaggcag cgcggtcatg atcgtgtagc cgtcgggctc ggcgcgtgcg
      301 acgtaagcgt gcccgatccc gcctcccgct ccgcccttgt tctcgacgac gaccctctgt
      361 cccaacgtgt cggcgagatg ggcggcgagc gggcgggcgg cgtagtcggc gacgccgccc
      421 ggcggaaacg gcaccacgag cgtgatcgtg cgcgatggat aggtttgcgc gtgcgcgatt
      481 atgggcgcgg tcgccgccgc gccggcgaag agcgtaatga actcgcggcg cctcatgtgc
      541 tccccttggt ttgagggatc acatgctatc acatcgctaa ggagaatgcc gcgttgtcgt
      601 ggaggcgatg cgttaatttc gtcgccgttt catcgactac cgtagcgcgt ccgcctcccg
      661 acgctcacgc tccgcgccct gacgctttgc ctcgagacat ccttccggca tgcggtatac
      721 ctcggtcgga ggttccgcct tccgatacgc cgccttccgg cgctgatact cgagctcctc
      781 ccgatccgcc tcgtaacact ccaggagctt gcgtcgctcc aggtcctgaa gccccgcggc
      841 ttgttgcgga gggatttcgt tgaaccggac cggcacggtg atgcggaacg tcgcatcgcg
      901 aaggccgacg tgatccggcg ctggaagtgg ctgtgattgc tgcaggagga ggagcgccgc
      961 actgccgagt tcacggtcaa cggtcaggat gcgggttccc ggcagcaccc acccggcgcg
     1021 gttgatgttc aagtcgaagt acacaacgcc gattttgccc cgggttctaa cgccgggcag
     1081 atagcgctta ttgtgttgaa tgtccgcggc gatttggttc ttccactcga ccagcgaaga
     1141 aaaatcgttt gccggcgcat cacgcgcgac cgcaaattca gcgcccgggg acaccagagc
     1201 cgatacgaca gcaaaaatgc cgagcaatcg cgatgtcagc atctcaaacc cgagccaaga
     1261 gtggatgaaa gttacatgac gattctgtgc taatcgttgc cgaaaaatct gctggagatg
     1321 gtttactgat ggcgaaaaaa tggccggccg ctctatcgac aaacgtgttg gccgcggcgc
     1381 tgtgcgattt cgccccgcgc tatgtcaacc cgagctccat cggctccggc ctcatggttt
     1441 atggttgctg acccggccgc ggccgagttc ccgaccagtg ggaggcccga tcggcacgcg
     1501 agcttcgagg caaatcgtgg gtccgatcga attattggca gaagtcccaa tagccccgag
     1561 tctgcgagga attcgaccaa taccagcaca cgtcgaaaga cggcgaggtc ggcacggcgc
     1621 tggcggcaat gatggcgccc aacgtgacac cgtcaaagac gctgccgtag taggattgcc
     1681 gcacccacgg ccgcacgggc ctcacgacct cggtggattt cgcggccacg gcgctgcgct
     1741 tggccaccgc gacatgggtc cttcgggttg cgacggcgcg atgccgaacc gcgacgtgac
     1801 gggttctgac cgcgacgcgt cgatgtccgc ttgcgacgtg acgggttctg acgtggctgc
     1861 ctcgatgtcg aaccacgacg tgacgggtta ccgtgcgatc tttgcgtagc gcgaccgttt
     1921 cggcgatggc cggcgttgcc gacgctggct gcaaacccga cggcggaacc accattgcgt
     1981 tggtgccctc ctggagagaa aggacaccca acgcgagcag cacggctgca attgacgttt
     2041 tggacactct cgctcctcct ctgttg
//
LOCUS       DATLJT010000083         1883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3679271,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000083 DATLJT010000000
VERSION     DATLJT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679271"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VM910_01755"
     CDS             <1..969
                     /locus_tag="VM910_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HVI61309.1"
                     /translation="EIRQRALAAIDLIGLDGYESAYPRELSGGMRQRVGFARALVVHP
                     NILLMDEPFSALDVLTAETLRTDFLDLWSEGRLPIKGVILVTHNIEEAVLMCDRVLVF
                     STNPAQIVSEITVDLPQPRNRLDPRFRDLVERIYVAMTERAAAAARATSLPERFPRIG
                     TDTVLARVSANLLSGLIEAVAGPPYNGKADLPVIASSLHMEVDDLFPVAETLQMLRLA
                     EIEKGDIRLTDVGKEFAGATLDDRKKLFQHQLLTHVPLAAHIRRVLQERANHIAPRSR
                     FLDELEDHMTTDAAEQTLRAVVAWARFAEAFAYDDDSQTFSLENPG"
     gene            1307..1690
                     /locus_tag="VM910_01760"
     CDS             1307..1690
                     /locus_tag="VM910_01760"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVI61310.1"
                     /translation="MRWFRSRARLGSGLALFALVAQLTLSFGHLHADASARDLGVAAA
                     ASNMQPSADQTSTPSDSSDHQSIPDDYCPICALIHLACGMVPAEAPPLPLRDHFGRLH
                     LELAVELGSSTPRATPFQARAPPTA"
BASE COUNT          349 a          586 c          565 g          383 t
ORIGIN      
        1 gagattcgcc agcgtgcgct tgccgcgatc gacttgattg gcctggatgg ctacgagtcg
       61 gcctatccac gcgagctgtc gggcggcatg cgccagagag tcggtttcgc acgcgctctc
      121 gttgttcacc ccaatattct gttgatggat gaaccgttct cggcgctcga cgtcctcacc
      181 gcggaaactc ttcggacgga ctttctcgat ctctggagcg aaggccggct tcccatcaaa
      241 ggggtcatcc tggtgacgca caatatcgaa gaagcggtac tgatgtgcga ccgcgtcctc
      301 gttttctcga ccaatccggc gcagatcgtc agcgagatca cggttgactt gccgcaacct
      361 cgcaaccgcc ttgatcccag gttccgtgat cttgtcgagc gaatctatgt cgccatgacg
      421 gagcgtgctg ccgcggcggc gcgcgcgacg tccctgccag aacgctttcc taggattggg
      481 actgacacgg tgctggcgcg ggtctcggcc aatttgctgt ccggtctgat cgaggcggtc
      541 gccggcccgc catacaatgg aaaggctgac cttccggtca ttgcaagttc acttcacatg
      601 gaagtggacg acctgtttcc ggtcgccgag acactgcaga tgctgcggct ggcggaaatc
      661 gagaaaggcg atattcggct gaccgatgtc ggcaaggaat tcgcgggcgc gacgttggac
      721 gaccgaaaaa aacttttcca gcatcagttg ctgacccacg ttccgcttgc cgcccatatc
      781 cggcgtgtgc tgcaggaacg cgccaatcac atcgcaccaa gaagccggtt tctcgacgag
      841 ctcgaagacc acatgaccac cgacgcggca gagcagacgt tgcgcgcagt cgtggcctgg
      901 gctcgctttg cggaagcctt tgcttacgac gacgactccc agaccttcag cctcgagaat
      961 ccggggtaac ggaatggagc gtcctaagca acacttgcag caattgtttg cgaatcaacg
     1021 gcgcctctcc acccggccgc gcgcgtctgg ggcttgacca aagagaacgc ttattagatc
     1081 gcggacagaa cggcagaaga cgggcatcat gtcatggcgg cgggtttcgg gaccagctca
     1141 tcaggcgaat cttccaggtt ccctgaaatt gccatcattt gggagcttgc cggagtcgag
     1201 cggtagcgag ccgtcgaacg ctcgttcctt ttacaagcgg ccaccaccga aagggcgggg
     1261 cgggctccaa ctgcctcagt gccatggcaa cgtaggcgta atgcggatgc ggtggttccg
     1321 atcgcgggcg agattgggct cgggcttagc gctgtttgcg ctggtcgcgc agctcacgct
     1381 ctcgttcggg catcttcacg ctgatgcgag cgcgcgagac cttggcgtgg cggctgcggc
     1441 ctcgaacatg cagccgtcgg ccgaccaaac gagtacgcct tccgacagct ccgatcatca
     1501 atctatcccc gacgattatt gcccgatctg cgcactgatt catttggcgt gcggaatggt
     1561 cccggcagag gcccctccgc tgccgctgcg ggaccatttt ggccggctgc atctcgagct
     1621 cgcggtcgaa ctcggctcga gcacgccacg tgcaactccg ttccaggccc gcgcgccccc
     1681 caccgcctaa cctcatcctg atctcgccct gcctcgcgca agcgaagatt gcgccggtat
     1741 ccatcgcgca atgttgcgcc ggcaatggtc ataggaacaa gttttcggtg gggatacccg
     1801 tgaaaaccat ctggcaacga agcgtacttc atgtcgctgt gctggccgct gccgtcatcg
     1861 gccttctgct cgtggcaggc ctg
//
LOCUS       DATLJT010000084         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3679298,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000084 DATLJT010000000
VERSION     DATLJT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679298"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VM910_01765"
     CDS             complement(<1..388)
                     /locus_tag="VM910_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI61311.1"
                     /translation="MLRVVVLGAAAGGGVPQWNCGCEVCRAARGEHPELKSTQASVAI
                     SADGEHWFLINASPDLRQQSIATPQLHPAKGRLRHSPIAGVILTNGEVDAVAGLLSMR
                     EGSPFAIYAHPKVLAVLKANSIFNVLS"
     gene            complement(530..610)
                     /gene="pqqA"
                     /locus_tag="VM910_01770"
     CDS             complement(530..610)
                     /gene="pqqA"
                     /locus_tag="VM910_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HVI61312.1"
                     /translation="MAWKAPKIVEVPCGMEINMYASAARK"
     gene            919..1176
                     /locus_tag="VM910_01775"
     CDS             919..1176
                     /locus_tag="VM910_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61313.1"
                     /translation="MPRYFFNTRIGDELIVDPEGEELRNADHAWEVARTMILELMRTE
                     GSTVLLLNAVIEVTDDDGEIVLEFPFSEAVLDLPDERVTRH"
     gene            1359..2777
                     /locus_tag="VM910_01780"
     CDS             1359..2777
                     /locus_tag="VM910_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVI61314.1"
                     /translation="MIARLSPRGLLLAGVFLGAFNLAAAAEDTPASPPAAAPVPAPAA
                     VPAPAPAETPAAAPAAAAPAPSPSPAAAAAPAPAAAPAPTTAAASPPALPPGSPMIGR
                     PDNEAALKLAPVAPPPLPAAADKLPAARLKLAPGFNIEVYASGMANARSLALGDKGTV
                     FVGSRLVDKVYAIVNKDGKREVKVLASGLYRPNGVAFHNGTLYIAELSKVSKIEHVED
                     VLDNPPKPTVIYDKLPKDEAHGWKFIAIGPDNKLYLEVGQPGNNVLHDDAHGQIRRIN
                     LDGTGAEVVALGVRHSVGFDWHPETKQFYFTDNGRDWLSEDVPEDELNRVTKVGEDFG
                     APYCYQGNIPDPEFGWGKSCSDYTPPVGLMGPHAGTLGMRFYTGSMFPKSFKNTIIVA
                     RHGSWNRSRKFGGDVVFVHLDKDGKVKSMEPLITGFLVDNSYIGRPVDVLQMKDGSIL
                     VSDDWNGAVYRVTYGKQKVAHQ"
     gene            2922..3488
                     /locus_tag="VM910_01785"
     CDS             2922..3488
                     /locus_tag="VM910_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61315.1"
                     /translation="MRKFWPVLALALLVPSVRAETIEERAVACFACHGEHGQSQTDNT
                     PSLGGQQAPYALIQLFMFREKLRTFEPMNEMAKPLSDGDLRDFSDFIARLPKPQPPAD
                     AGDPSRMQRAQALVVQNHCNSCHRPDFSGGDNVPRIANQREDYLAKTLKEYKDNTRHG
                     YDATMADVMQPVTPEQIADLAYYIARVR"
     gene            3647..5047
                     /locus_tag="VM910_01790"
     CDS             3647..5047
                     /locus_tag="VM910_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI61316.1"
                     /translation="MQDLWRLSAIDLAALVRSKKVSAKEAVAAALARLDAVNPKINAV
                     VDHRSDDVLKEASRVDAAIARGEDAGPLAGVPVTVKVNIDQEGFATTNGLKLQAGAIA
                     KSNSPVIDNLRKAGAVILGRTNCPAFSYRWFTTNLIHGDTKNPRDPGVTPGGSSGGAG
                     AAVAAGIGHIAHGTDIAGSIRYPAYACGVHGLRPTVGRIAAFNAALPERTIGPQISAV
                     SGPLARTIADLRIALAAMSSKDVRDPWWVPAPLEGPPVQKRAALCLNPDGLDPVPEVK
                     AAVADAGKRLARAGWTVEEISNTPPLREAADWQTKLWLGDGYEAQLEAAEKEGDPGAL
                     ACLRGNRANVFPFDQAAFSKALTRRATLTREWLQFFESYAVLLMPVSGELPFPDNLDR
                     KDGASFARVWRAQLPQIAIPFMGLPGLTVSTGLVGKIPVGVQVVSGRYREDLCLAAGE
                     AIEAGGAPPSPIDPVS"
     gene            5229..5705
                     /locus_tag="VM910_01795"
     CDS             5229..5705
                     /locus_tag="VM910_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HVI61317.1"
                     /translation="MSGVYDFTATSLSGDEVPLKRFEGQVLLIVNTASKCGFTPQYQG
                     LEALQREFSPRGFAVLGFPCNQFGGQEPGDARQIADFCESKYDVTFPMFSKIDVNGFD
                     AHPLYDYLKKEKSGLLGPSIKWNFTKFLVDRAGRVVARHAPTTTPDALKKEIEALL"
     gene            5717..5956
                     /locus_tag="VM910_01800"
     CDS             5717..5956
                     /locus_tag="VM910_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HVI61318.1"
                     /translation="MDQLPDRLSVDPASPYYNAEILARDVGIRFKGIEKTNVEEYCVS
                     EGWVRVTAGNAKDRHGNPLTIKVHGPVEPYFRDKK"
     gene            6104..>6311
                     /locus_tag="VM910_01805"
     CDS             6104..>6311
                     /locus_tag="VM910_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HVI61319.1"
                     /translation="MSVRITDVCEVTKPISSPIRNAYIDFTKMTSSLVAVVTDARRDG
                     KRVVGYGFNSNGRYGQGGLIRERFA"
BASE COUNT         1235 a         2012 c         1923 g         1141 t
ORIGIN      
        1 cgctcagcac attgaagatg ctgttggcct tcagcactgc cagcaccttg ggatgcgcat
       61 agatcgcaaa gggcgaaccc tcgcgcatcg acaacaatcc cgcgaccgca tcgacctcgc
      121 cattggtgag aatgaccccc gcgatcgggg agtggcggag ccggcccttt gcgggatgaa
      181 gctgcggggt tgcgatcgac tgctggcgca ggtcgggcga ggcattgatc aaaaaccagt
      241 gctcgccgtc ggcactgatc gcgaccgagg cttgcgtgct cttcagttcg ggatgttcgc
      301 ctcgggctgc ccggcatact tcgcatccgc aattccactg cggaacgccg ccgcctgccg
      361 ccgcgcccag gacgacgacg cgaagcatga tccgtcccct ggaaccttca ttcaaagact
      421 gcacgtaaaa attcaagaag gtggatacgc gccccggtca cgccccctgc aggcacgtgg
      481 tcgcggcccg aggccacctt gcgaagaacg cgaagctgtc gccggctaat tacttgcggg
      541 cggcagatgc atacatgttg atttccatgc cgcagggcac ttcgacaatc ttcggtgctt
      601 tccaggccat tttggctctc cttgttgacc cttgttgcaa acggacgacc tcgccctctc
      661 cattaaatta acgcgcaacc gcaagttagc cagctaaatc tttgttgaga gagccgcgcc
      721 ttttgttgcg ctgcaatagc gagtttgggc gtcgcagccg ccaaaagttc caaatcaaaa
      781 cgggatcaat ggtttcgttc cctgttccat cggcgcaagg ctgatcggca tcggacgaga
      841 tgcgggaacg gcgaataaag aagttgtgag agaccggccg cttccaattt ccagcgtttc
      901 gccccaattg cactggctat gccgagatat tttttcaaca cgagaatcgg cgatgagctg
      961 atcgtcgacc ccgagggcga agagttgcgc aacgcggacc acgcctggga agtcgcgcgc
     1021 acgatgatcc tcgagctgat gcgaaccgaa ggttcaaccg tgctgctgtt gaatgccgtt
     1081 atcgaggtga ccgacgatga cggggagata gtgctggagt ttccgttttc ggaagcggtc
     1141 ctcgacctgc ccgacgagcg ggtgacgcgg cactgatacg ccgccgctac cccgaccccg
     1201 agatccattc cggcgcccct tccgattgat cgcggacgcc ttcgtcatgt ttccgattgc
     1261 gcaagggacg aaaaatataa caaactgaag caggaaggcc gaccccggaa cttaaccggt
     1321 tgcgaggagc tgcggccgcc atgcaagggg aggatgacat gatcgctcga ttgtcgccgc
     1381 gcgggctttt gctcgcgggc gtttttcttg gtgcattcaa tcttgccgca gccgcagagg
     1441 acacgccggc ctcgccacct gccgccgcgc ccgtgcccgc acctgccgcg gtacctgcgc
     1501 cggcgcctgc cgagacacct gcagcagcac cggccgcggc agcacctgcg ccgtcacctt
     1561 ctccggctgc cgccgcggct cccgcaccgg ccgcagcgcc ggctccgacc accgccgcag
     1621 cgtccccgcc cgcgcttccg ccgggttccc cgatgatcgg ccggccggac aatgaggccg
     1681 ccctgaagct tgcgccggtc gcgccgccgc cgctgccggc cgccgccgac aaactgccgg
     1741 ccgccaggct caagctggcg cccggcttca acatcgaggt ctacgcctcg ggcatggcga
     1801 acgcgcgttc gctcgcgctc ggcgacaagg gcaccgtgtt cgtcggcagc cgcctcgtcg
     1861 acaaggtcta tgccatcgtc aacaaggacg gcaaacgcga ggtcaaggtg ctggcgtccg
     1921 gcctctatcg gccgaacggc gtcgccttcc acaacggcac gctctacatc gcggaactct
     1981 caaaggtctc caagatcgag catgtcgagg acgtcctcga caacccgccg aagccgaccg
     2041 tcatctacga caagttgccg aaggacgagg cgcatggctg gaagttcatt gcgatcggcc
     2101 ccgacaacaa gctctatctc gaggtcggac aacccggcaa caacgtgctt catgacgatg
     2161 ctcacggcca gatccggcgc atcaatctgg atggaacagg cgcggaagtg gtcgcccttg
     2221 gcgttcgcca ctcggtgggc ttcgactggc atcctgagac caagcagttc tacttcaccg
     2281 acaacggccg cgactggctg tcggaggatg tgcccgagga tgagctcaac cgcgtcacca
     2341 aggtcggcga ggatttcggc gcgccctatt gctaccaagg caatatcccc gacccggaat
     2401 ttggctgggg caaatcctgc agcgactaca ccccgccggt cggcttgatg ggaccgcatg
     2461 ccggcacgct cggcatgcgc ttctacaccg gcagcatgtt ccccaagagc ttcaagaaca
     2521 cgatcatcgt cgcgcgccac ggctcctgga acaggagccg caaattcggc ggcgacgttg
     2581 tcttcgtcca tctcgacaag gacggcaagg tcaagtcgat ggagccgctg atcaccggtt
     2641 tccttgtcga caacagctat atcggccgcc ccgtcgacgt gctgcaaatg aaggacggct
     2701 cgatcctggt ctccgacgac tggaacggcg cggtctaccg cgtcacttac ggcaagcaga
     2761 aggtcgcgca tcaataggga tgtcaggtaa cgacgtgatt ccgagccgaa ggtccccgcg
     2821 aagcgcggcc cgatgacagg ctccgcggag caatccagtg cgacgattcg attctggatt
     2881 gcctcggagc tgctcgcaat gacgaaagta gagaccagtt gatgcgcaaa ttctggcctg
     2941 tactcgctct ggcgctcctc gtcccttctg ttcgcgcaga aaccatcgag gagcgtgcgg
     3001 tcgcctgctt cgcctgccat ggcgagcacg gccagtcgca aaccgataac accccttcgc
     3061 tcggcggtca gcaggcgcct tatgcgttga tccagctctt catgtttcgc gagaagttgc
     3121 gaaccttcga gccgatgaac gagatggcga agccgctgag cgacggcgac ctccgcgatt
     3181 tttccgattt catcgccaga ttgccgaagc cccagccgcc tgcggatgcc ggcgatccct
     3241 cgcggatgca gcgggcgcag gcgctggtgg tgcagaatca ttgcaattcg tgccaccggc
     3301 cggatttctc cggcggcgat aacgttccgc gcatcgccaa ccagcgcgag gattatcttg
     3361 cgaagacgct gaaggaatac aaggacaaca cccgccacgg ttatgacgcc accatggccg
     3421 acgtgatgca gccggtgacg cctgagcaga tagccgatct tgcctattac atcgcccgcg
     3481 tgcggtagcg cttgatcacc cgggagaggc aagggggaga gagtcgcctt ggttggcgcc
     3541 gccgtgtgtt ttgcagacat ccggcaagcg ggaaagggag acggggccac gactggcctt
     3601 gctgttgcag cggcgctaaa agaccaagca tcacacggag caagccatgc aggatctctg
     3661 gcgcctctcg gccatcgacc tcgccgccct cgttcgatcg aaaaaggtct cggcaaagga
     3721 ggcggtcgcc gccgcgctgg cgcggctcga cgcggtcaac ccaaaaatca acgccgtggt
     3781 cgatcatcgc tccgacgacg tgttgaagga ggcttcacgt gtcgatgcgg ccattgcgcg
     3841 cggcgaagat gcggggccgc ttgcgggcgt cccggtcacg gtcaaagtca atatcgacca
     3901 ggaaggcttt gccaccacca acgggctgaa gctgcaggcg ggcgccatcg ccaagagcaa
     3961 cagcccggtg atcgacaatc tgcgcaaagc gggcgccgtc atattgggcc gcaccaattg
     4021 tccggccttt tcctatcgct ggttcaccac caatctgatc catggcgaca ccaaaaaccc
     4081 gcgcgatccc ggcgtaacgc cgggcggctc ctccggcggc gcgggtgcgg cggtcgcggc
     4141 cggcatcggt cacatcgcgc atggtaccga tatcgcgggt tcgatccgct atcccgccta
     4201 tgcctgcggt gtgcatggtc tgcggccgac cgtgggacgc atcgcggcct tcaacgccgc
     4261 cttgccggag cgcaccatcg gtccccagat ctcggcggtg tcgggtccgc tcgcgcgcac
     4321 gatcgccgat ctgagaatcg cgctggccgc aatgtccagc aaagacgtgc gcgatccctg
     4381 gtgggtgccg gcaccgctgg aagggccgcc cgtgcaaaag cgtgccgcac tgtgcctcaa
     4441 cccggacggg ctcgatcccg ttcccgaggt taaggctgcg gtcgccgatg ccggcaagcg
     4501 gcttgcccgc gccggctgga ccgtcgaaga gatttcaaac acgccgccgc tgcgcgaagc
     4561 cgccgactgg cagacaaaac tctggctcgg cgacggctat gaggcgcaac tcgaagccgc
     4621 ggagaaggag ggcgatcccg gcgcgctcgc atgtctgcgc ggcaaccggg caaacgtgtt
     4681 tcccttcgat caggcggcgt tctcgaaggc gctgacccgc cgcgccacac tgacgcgcga
     4741 atggctgcaa ttcttcgaga gctatgcggt gctcttgatg ccggtctccg gcgaactgcc
     4801 gtttccggac aatctggatc gcaaggatgg cgcctccttt gctcgcgtgt ggcgcgcaca
     4861 attgccgcag atcgctattc ccttcatggg gttgcctggt ctaaccgtct caaccggact
     4921 ggtcggaaag attcctgtcg gcgttcaggt cgtctccggc cgctaccgcg aggatctttg
     4981 ccttgccgcc ggcgaagcga tcgaggcggg cggcgcgccg ccatcgccga tcgatccggt
     5041 gagctagagt acgatgtggt caggcaattg aaacggaagt gcgctccctc cccctgaaag
     5101 agggagggtt ggggaggggg gtctgatcac cttccgcttt gctcctcgcg gagcaaagcg
     5161 acctccccct tttcaagggg agtttaggaa tagcaaggac tgagcgatca agcgaacgag
     5221 ggaactggat gtcaggcgtt tacgatttca ccgctacatc gctgtctggc gacgaggtgc
     5281 cgctcaagcg gttcgagggc caggtgctcc tgatcgtcaa caccgcgagc aaatgcggct
     5341 tcaccccgca atatcagggg ctcgaggcac tgcagcgcga attttcgccg cgcggctttg
     5401 ccgtgcttgg ctttccctgc aatcagttcg gcggccagga accgggtgac gccaggcaga
     5461 tcgccgattt ctgcgaaagc aaatacgacg tcacctttcc aatgttttcc aagatcgacg
     5521 tcaacggttt tgacgcgcat ccgctctacg actatctgaa gaaagagaaa tcgggtctgc
     5581 tcgggccctc catcaaatgg aatttcacga aattcctggt cgaccgtgcg ggcagggtgg
     5641 tcgcgcggca cgcaccgacg acgacacccg atgcgctgaa aaaagagatc gaggcactgc
     5701 tgtgaacgat gacaacatgg atcaactgcc agaccgcctg tcggtcgatc cggcaagccc
     5761 gtattacaac gcggagattc tcgcgcgcga cgtcggcatc cgcttcaagg gcatcgagaa
     5821 gaccaatgtc gaggaatatt gcgtcagcga aggctgggtg cgcgtgactg cgggcaatgc
     5881 caaggaccgc cacggcaatc ccctcaccat caaggtgcac ggcccggtcg agccatattt
     5941 tcgcgacaag aagtgacggt gctggcgtca aaccggcgct ctttgtataa ctccatcatg
     6001 gccgggcctg tcccggccat ccacgtcctt cgcgcagaga aagaaaaggc gtggatgccc
     6061 gggtcaagcc cgggcatgac gaaaacaacc tgtgaactta atcatgtccg tccgcatcac
     6121 ggatgtctgc gaagtcacaa agccgatctc ttcgccgatc cgcaacgcct atatcgattt
     6181 cacgaagatg acgtcgagcc tcgtcgccgt cgtcacagat gccaggcgcg atggcaagcg
     6241 cgtggtcggc tacggcttca attcgaacgg tcgttacggc cagggcgggt tgatccgcga
     6301 acgctttgcg c
//
LOCUS       DATLJT010000085         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_922535,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000085 DATLJT010000000
VERSION     DATLJT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922535"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="VM910_01810"
     CDS             complement(<1..2425)
                     /locus_tag="VM910_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HVI61320.1"
                     /translation="MRPLLAPEVRAIMSLPRGPQRDRAPDWIAAGTPPRLRGDLVALL
                     GSDRVLTRPIDLIRYATDASPYRFFPKVVVIARTVDDVRKVLDYARQKNETVTFRSAG
                     TSLSGQAQSDGILVDVRRHWAGVNIEAGGRRLRARPGTILSRANLALVAHGYRLGPDP
                     ASASACTIGGVIANNSSGMCCGTTQNSYKTLSSLTFMLPSGTLIDSARANAEQQFSAT
                     EPGLVAGLMEIKREIEADPELVARLRKKFSIKNTTGYHMEAFLDGSTPLEIFRRLIVG
                     SEGTLAFIAEAVFDTVPDDQYRLTSFMIFPDMYAACAAVKPFVDHGAAAVELVDRASL
                     RSVEGKPGVPDRWKTLPEQATGLLVEFRAPSEVGRADAERTAGATLAELKLLEPAEFT
                     RDPVLAAQFWNVRSGLLASVGGARPSGSSFILEDVCFPAEGLADGALDLQALFARHGY
                     AGIVFGHASAGNLHFLITPFLNDPKEIAHYGAFMQDVVELVVGKYDGSLKAEHGTGRN
                     VAPYVEREWGGKLTGLMWKLKRLADPKNILAPGVLLTDDPESHLKNLQTSPIIEDTVD
                     RCIQCGYCEHVCPSRNLTTTPRQRIALRREMARQPEASSVSAQILAEYDYDAIQTCAG
                     DGSCAMACPVNINTGALMKHFRYLEHGPRAERIAATMARRFGSVERVARIGLAVGDAT
                     AKVLGHRTLAAATALARAFVSKDLVPSWLPNMPAAANAQLPKTSRRAAAGVYFTACVN
                     RIFGRMPGQGRKPSLQEAMIAVSARAGLPLWLPEDIGGHCCATIWHSKGYAKGNSIMA
                     NRIAESLWRW"
     gene            complement(2816..3142)
                     /locus_tag="VM910_01815"
     CDS             complement(2816..3142)
                     /locus_tag="VM910_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61321.1"
                     /translation="MDGHTKLRCFSQYLIVGAIVAATPAFAADPTNGRRLAERWCVAC
                     HVVTPAQVRAATDQAPPFATIAKTSDFDAAKIALFLLDPHPKMPDMALSRSEAADLAA
                     YIVSLK"
BASE COUNT          708 a         1122 c         1188 g          694 t
ORIGIN      
        1 tccaacgcca cagactttcg gcgatgcggt tcgccataat ggagttgcct ttggcgtagc
       61 ccttggaatg ccagatggtc gcgcaacaat ggccgccgat gtcttcggga agccacagcg
      121 gcaacccggc gcgcgccgag acagcgatca tggcctcctg cagcgaaggt ttgcgcccct
      181 gcccgggcat ccggccgaaa atgcggttga cacacgcagt gaagtagacg ccggcggcgg
      241 cccggcgcga cgtcttcggc agctgagcgt tcgcggcagc cggcatgttc ggcagccagc
      301 tgggcaccaa atccttgctc acgaaggcgc gggccagcgc ggtcgccgcc gcgagcgtgc
      361 ggtggccgag cacttttgcc gtcgcatcgc cgacggcgag accgatgcgt gctacgcgct
      421 caaccgagcc aaatcgcctc gccatagtcg cggcgatgcg ttcggcgcgc gggccgtgtt
      481 cgagatacct gaaatgtttc attaacgccc cggtattgat gttcacgggg caggccatcg
      541 cgcaggaccc atcgcccgcg caggtctgga tggcgtcata gtcgtattcg gcgagaatct
      601 gcgcggatac gcttgaggct tcgggttgtc gggccatctc acggcgcagc gcgattcgct
      661 ggcgcggggt ggtggtcaga ttgcggctgg ggcagacgtg ttcgcaatag ccgcactgga
      721 tgcagcgatc gacagtgtcc tcgatgatgg gcgaagtttg caggttcttc agatggcttt
      781 ccggatcgtc agtgagcagg actcccggtg caagaatatt cttaggatcg gcgaggcgct
      841 tcagcttcca catcaagcca gtaagttttc cgccccattc gcgctcaacg tacggcgcga
      901 cgttacgccc ggtgccgtgt tcagccttga gtgagccgtc atatttgccg acaaccaatt
      961 cgacgacgtc ctgcatgaac gcgccgtagt gcgcgatttc cttcggatcg ttgagaaacg
     1021 gcgtgatgag gaagtgcagg ttgccggccg aggcgtgccc aaaaacgatt ccggcgtagc
     1081 cgtgcctggc gaacagcgcc tgcaaatcga gcgcgccgtc ggcgagcccc tccgcgggaa
     1141 agcatacgtc ctctaaaatg aaagacgatc cagaggggcg cgcccctccg accgatgcta
     1201 agagcccgct gcgcacgttc cagaactgcg ccgccaacac cggatcgcgg gtgaattcag
     1261 ccggctcgag cagcttcagc tcggccaatg tcgcgccggc agtgcgctcg gcgtcggcgc
     1321 ggcctacttc actgggcgcg cgaaattcga ccagcagtcc cgtcgcctgc tcgggcagcg
     1381 ttttccagcg gtccggcact cccggcttgc cttccaccga acgcagcgac gctcgatcga
     1441 ccagctcgac tgcggccgcg ccatgatcga caaacggctt caccgccgcg cacgccgcgt
     1501 acatgtccgg gaagatcatg aacgaggtga gccggtattg atcgtccggg acagtgtcga
     1561 aaactgcctc ggcgataaac gccagcgtgc cctccgagcc gacgatcaga cggcgaaaaa
     1621 tctcaagcgg cgtggagccg tcgaggaacg cctccatgtg gtagccggtc gtattcttga
     1681 tgctgaattt tttgcgcagc cgcgccacga gttcagggtc ggcttcaatc tcacgtttga
     1741 tttccatcaa cccggcgacg aggcccggct cggtggccga gaattgctgc tcggcattgg
     1801 cgcgtgcgct gtcgatcaac gtgcctgagg gcagcatgaa cgtgagcgac gacagcgtct
     1861 tgtaggagtt ctgcgtcgtg ccgcagcaca tgccgctgga attgttggcg atgacgccgc
     1921 cgatggtgca agcggatgcg cttgccggat cgggaccgag gcgatagccg tgcgccacga
     1981 gcgcgaggtt ggcacgcgac aggatggtgc cgggacgcgc gcgcaggcgc cgtccgcctg
     2041 cttcgatgtt gacgccggcc caatgtcgcc gcacgtcgac caaaatgccg tcgctctgcg
     2101 cttggccgga gaggctcgtc ccggcggaac ggaacgtcac ggtctcattc ttttgccgcg
     2161 catagtcgag cactttgcgc acgtcgtcga cagtgcgcgc gatcaccacc actttgggga
     2221 aaaatcgata aggactcgcg tcggtcgcgt agcggatcag atcgatgggt cgcgtgagca
     2281 cgcggtcgga gccaaggagc gccacgagat cgccgcgcag ccgcggtggt gttcccgccg
     2341 cgatccaatc cggcgcacgg tcacgttgtg ggccgcgagg tagcgacatg atggcccgga
     2401 cctcgggggc gagcagtggg cgcatagcga agtgatccag ccgggatatg gtgatctgtg
     2461 gaagacgtta tgctagatca aagccggtcg ggccggagcg ggaatctgag ttgcccgctt
     2521 cagccaagcc gttccattgt acgccgaaat cggctcgagg tccgtactgc gatcgattgg
     2581 ttcgccagca gatcggccgt gataatcgcg accggagtta ctgtatcaga cgccgtgaac
     2641 ttcctcctgt cggccaacga tgtccgcagt ccgacggtgc tgcggatctc gcggccggaa
     2701 aggctctcgc cgccctacag cgcgcatttc gacttgacgg tcgatcgaag cgacaccggt
     2761 cctcggtacc agcgaaaatg atggagggat gggtcaatgc cacacgccat tggtgttatt
     2821 tgagacttac aatatatgct gcgaggtctg ccgcttcgga tcgagatagt gccatgtccg
     2881 gcatcttcgg atgcgggtcg agcaagaaca aagcgatctt ggccgcatcg aaatcggatg
     2941 tcttcgcaat cgtcgcgaac ggtggcgcct gatcggtggc tgcgcgaact tgcgcgggcg
     3001 tcaccacatg gcaagccacg caccatcgct cggcaagccg tcgaccattg gtgggatcgg
     3061 cagcaaatgc cggggtggcc gccacaatcg caccaactat caggtactga gaaaagcagc
     3121 ggagctttgt gtgtccatcc attggggaca atctcctcgc tttggggcaa gagtaagatt
     3181 cacctcggaa taaatggtcg aatgagagtg gaatgagacg ctcctgggcg cttcctggag
     3241 gcttcacatg ctcatgcttt cacgcattct tatcacgttc gtgagcttga ttacgctaat
     3301 cggctcgaat atcgcagatt ggaacagcac gccgccccgg aattgtggca aaggtcgacg
     3361 ccgatctgag aatcctcgag catcttgccc atctcgccga gcgagagata ccggagccgc
     3421 ggcgctaccg gcctgtcgac gcgtgggcca actgcgccgc tcgctggagc gcgagcttga
     3481 tctcgcagtt gaggcacgca acacggaacg cttcgcccgc aactttgctg gcgatctcga
     3541 cgtgctcgtc ccgcgcgtct atatggacga ccagctcgat gaatgtgcaa gagcacatcg
     3601 agggcatccg cggcgacgat ttagaggcca tcgacaacgc tggcctcgac cgcaaggcga
     3661 tcggcgcgcg cggcgtggat accatgctca aaatgatcct gatcgacggc tt
//
LOCUS       DATLJT010000086         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1381972,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000086 DATLJT010000000
VERSION     DATLJT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381972"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1348)
                     /locus_tag="VM910_01820"
     CDS             complement(308..1348)
                     /locus_tag="VM910_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI61322.1"
                     /translation="MEAVAHSELAKLSLDLKTLNAKPLWERTTRMGPGSPAVPAIWRY
                     RDMRPQLLRAIELITAKEAERRVFMLENPGLPGSGYITNSLYCGLQVIKPGEIAPAHR
                     HSPNALRFIIEGEGAYSTVEGERVPMHPGDFVLTPGWTWHDHGHLGSAPVIWLDALDN
                     PFGQFFGATFRENYPGDTHPVSQVEGAAAARYGSNLMPVQYRPQRRASPLLVYPYDRT
                     REALDRVARNEPLHPAHGAKMRYANPTDGGYVYPTLAVFIQWMPKGFSGQTYRSTDGA
                     VFNVVEGHGRAQIGEDDFAFEPHDVFVVPPWTSYYIVTESECVLFSYSDRAAQEALGF
                     WREEDPANRSAR"
     gene            complement(1378..2364)
                     /locus_tag="VM910_01825"
     CDS             complement(1378..2364)
                     /locus_tag="VM910_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61323.1"
                     /translation="MCAIVPSAPRVLLLVLTAVLALAASIPYALAQSWPQRTVKLIIP
                     LPPGTGTDIAGRLLAERLAGRWGQPVVVENKQGGDGIPAVTAFLAARDTHTLLLSFAG
                     IITINPLTHERLPYDPAADLVPLVQLSDNFLGVAASATLKVESLADLIRVARGQPGKL
                     NWAATPGLPYYVVLALEQSSGIEMVPVGYRDFAPALQDLATGRLHVAATGVPPLVPHH
                     QASTAKLLFVTNRERSPQAPEVPTASEAGYPDLTFEGSVGLFGWRDMPAYVRERIVRD
                     VQTVAADSAFRSRLIAGGTVPRSGTSEEFAAAIEEQRKKIAAIHRAFAKPAQ"
BASE COUNT          483 a          762 c          809 g          456 t
ORIGIN      
        1 tatctcgcca tcaatctgaa ggccgccgcg gcaatcggca ttgcgctgcc aacgtcaacc
       61 ttgctgcgcg ccgacgaggt gatcgaatag agacaccgct ttgctgcatc tgctcacagc
      121 cgcttgtggc acaaagcgga cttagcgaga tgtctgtcta tttgtccgct tccggggcga
      181 agcggacgtg catgggcgtg tggcttcgac cgcctcggtc gcggatgacc caacgcggat
      241 ggctcaacat cataagtcag ctctgcgctg aaagtccgat tgccgataat gcaacccctc
      301 cagcccctta cctggcagac ctattcgccg ggtcttcctc gcgccagaaa ccgagcgctt
      361 cctgcgcggc gcggtcggaa tagctgaaca ggacgcattc gctctcggtc acaatgtaat
      421 aggatgtcca cggcggcacc acgaagacat cgtgcggttc gaaggcaaaa tcgtcttcgc
      481 cgatttgtgc acgtccatgg ccctcgacca cgttgaacac ggcaccgtcg gtgctgcgat
      541 aagtctggcc ggaaaatcct ttcggcatcc attgaatgaa gacggcgagc gtcggataca
      601 cataaccgcc gtcggttggg ttggcatagc gcatcttcgc gccgtgcgcg ggatgcagcg
      661 gctcgttgcg cgctacgcgg tcgagtgcct cgcgcgtgcg gtcgtaagga tagaccagaa
      721 gcggagacgc gcggcgctgc ggccggtatt gcaccggcat cagattcgaa ccatagcgcg
      781 cggctgcagc gccctcgact tgagagaccg gatgggtatc gccgggatag ttttcgcgaa
      841 aggtggcgcc gaagaactgg ccgaacggat tgtcgagcgc gtccagccaa atcaccggcg
      901 ctgagccgag atggccgtgg tcgtgccagg tccagccggg ggtgagaacg aagtctccgg
      961 gatgcatcgg aacgcgctcg ccctcgaccg tggagtaggc gccttcgccc tcgatgatga
     1021 aacgtagcgc attgggcgaa tgccggtgag cgggcgcgat ctcgccgggt ttgatcacct
     1081 gaagcccgca atagagcgag ttggtgatgt agccgctacc aggcagtcca gggttctcca
     1141 acatgaagac tcggcgttct gcttccttgg ccgtgattag ctcgatggcg cgcagcaatt
     1201 gcggacgcat gtcgcggtag cgccagatgg ccggaacggc cggcgagcct ggccccatcc
     1261 gcgtcgtgcg ctcccagagc ggctttgcgt tgagcgtctt gagatcaagc gagagctttg
     1321 ccagctccga atgggccacg gcttccatgc gatccgtctc ctgtcgaaca gccgtttcta
     1381 ctgcgctggc ttggcgaagg cgcgatgaat ggctgcaatc ttcttgcgtt gctcctcaat
     1441 ggcggcggcg aattcctctg acgtgccgga gcgcggcacc gttccgcccg ctatcaggcg
     1501 agagcggaat gccgagtcgg cggcgacggt ctgcacatcg cgcacaatgc gttcccttac
     1561 atacgccggc atatcgcgcc acccgaagag gccgacactg ccttcgaaag tgagatcagg
     1621 atagcccgcc tcgctcgctg tcggcacctc gggggcctgt ggagaacgct cgcggttcgt
     1681 cacaaacagg agctttgcgg tgctggcttg atggtgggga acgagaggcg gcacccccgt
     1741 cgcggcgaca tgcagacgtc ccgtcgccaa atcctgcagt gccggggcaa aatcgcggta
     1801 gcccaccggc accatctcaa ttccgctgct ctgctccagc gcgagcacga cgtaataggg
     1861 caggccgggc gtggcagccc aattgagctt gcctggctgc ccacgggcga cacggatgag
     1921 atccgcgagc gactccactt ttaaggtcgc ggacgcggca acgccgagaa agttatccga
     1981 tagttggaca agggggacca ggtctgcagc gggatcgtag ggcagccgct catgtgtcag
     2041 cggattgatg gtgatgatcc cagcgaacga aagcaggagg gtatgggtgt cgcgtgctgc
     2101 aaggaatgcc gtgaccgccg ggatgccgtc gccgccttgt ttgttttcca cgaccactgg
     2161 ctgaccccag cgccccgcca gccgttccgc aagcagccgg cccgcaatgt ccgtgccggt
     2221 ccccggcggg agcgggatga tcagcttgac cgtacgttgc ggccagctct gggcgagcgc
     2281 atacggaatg gacgcggcaa gcgctaatac agcggtgaga actagcagaa ggacgcgcgg
     2341 agcggaggga acgatcgcac acacgacggc ctcctgactt gcggactctc gttgcacgca
     2401 cggcgcagtt ccggagcccg actgactgct actgcgtagc gtaacgaatt gtaaattcga
     2461 tcacaatcgc gcgctgcaag gcgtgatcaa cgccaagaca tcgatcgggc
//
LOCUS       DATLJT010000087         1734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_922550,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000087 DATLJT010000000
VERSION     DATLJT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922550"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..271)
                     /locus_tag="VM910_01830"
     CDS             complement(2..271)
                     /locus_tag="VM910_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI61324.1"
                     /translation="MASEPAKSDEMPGADIAHHLARHGVKVEVDSIVAVETDVASTTL
                     SHAADISADFLVMGGYGHSRLREFVLGGVTRGILGSMTLPTLMSH"
     gene            complement(196..831)
                     /locus_tag="VM910_01835"
     CDS             complement(196..831)
                     /locus_tag="VM910_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI61325.1"
                     /translation="MIKDIVANLAVGVSRDVATDFAVSAAAMLDSHLTGIAFRYEPLV
                     PLTDRYGFPAEVMDSQRVENEKRAKAAMVAFDEAARRAAVSAQSRMLDVPVASAPDVF
                     AKIARRFDLAIMGQPEPDTPALERLLVEAALFDSGRPVLIVPYIQRSGLTLERVMVCW
                     DGSRSAARAVADAMPLLVRAKATEVLTWPASRQRATRCPGRTSRTISPATV"
     gene            complement(909..1202)
                     /locus_tag="VM910_01840"
     CDS             complement(909..1202)
                     /locus_tag="VM910_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61326.1"
                     /translation="MLATVLIAANMCLQGGERIDISRYVDPTASLLELKVTMERTTGT
                     LVVYSPGYEEQQARFSDPESSALIPFSEPVLCVAAIGGPFEFNIEVMPLDNAD"
     gene            1354..1659
                     /locus_tag="VM910_01845"
     CDS             1354..1659
                     /locus_tag="VM910_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61327.1"
                     /translation="MQLLRFCSLLPAIVAASGALAADADNGKRLAETRCVTCHIVSPS
                     ERRDVTDAPPFEAIARKFASNPDTLAFSIVDPHPRMNVTLTRREAQDVAAYINTLAK"
BASE COUNT          354 a          534 c          515 g          331 t
ORIGIN      
        1 ctcaatgcga catcagcgtc ggaagagtca tggagcccag gattcctcgc gtaacgcctc
       61 cgagaacgaa ctcacgcaga cgcgaatggc catagccgcc catgaccagg aaatctgctg
      121 agatgtcggc ggcatgcgat agggttgtgc tggcgacgtc ggtctcgacg gcaacgatcg
      181 agtcgacttc gaccttcaca ccgtggcggg cgagatggtg cgcgatgtcc gccccgggca
      241 tctcgtcgct ctttgccggc tcgctggcca cgtcaggacc tctgtagcct tggctcgcac
      301 cagcaacggc atggcatcgg ccacggcacg ggcggcactc cggctgccat cccagcacac
      361 catgacgcgt tcgagcgtca gaccgcttcg ttgaatatac ggaacgatga gcacgggcct
      421 accggagtcg aagagcgccg cctcgacgag gagccgctcg agcgcgggcg tgtcaggctc
      481 cggctggccc atgatcgcca aatcgaaccg ccgcgcgatt ttggcgaaaa cgtccggggc
      541 gctcgcgaca ggtacgtcga gcatgcgcga ttgggccgaa acggcggcgc gtcgcgccgc
      601 ttcatcgaac gcgaccatgg cggccttcgc ccgcttttca ttttcgacgc gctgagaatc
      661 catcacttcg gcgggaaaac catacctgtc ggtcagaggc acgagcggct cgtagcggaa
      721 ggcgattccc gtaaggtgcg aatcgagcat ggcggcggcc gaaacggcaa aatccgtcgc
      781 cacgtcgcgg gagacgccca ccgctagatt cgcaacaatg tccttgatca tgattgaccc
      841 ccggccttgg acgcgatttg tcctgcgatt tgcgacgatg ggatgcagag ttcatgtcgc
      901 gaataggatc agtccgcatt gtcgaggggc atgacttcga tgttgaattc aaatggacct
      961 ccgatggccg ctacgcacaa gacgggttcc gaaaacggta tcaaggcgga tgattctgga
     1021 tcggaaaacc gcgcctgctg ctcctcatag cccggcgagt agaccaccag ggttccagtt
     1081 gtgcgctcca tggtcacctt gagttccaga aggctcgcag tcggatcaac gtaccgcgaa
     1141 atatcgatcc gctcgccgcc ttgcaggcac atatttgcgg caattagaac cgtggccagc
     1201 atggctgcct cctgcaggtt ttaaccaatg tcgaggcgac cagaatggcc gtctttgacc
     1261 tgcatcaact ttgcagtatc cagtcagttt catggtattg cgtcggcaag tgagcctcag
     1321 agcaatcagc ccaaattttt gacggaggtt ttcgtgcagc tgcttcgctt ttgttcgctt
     1381 cttcccgcga tcgtggctgc atcgggcgcg cttgctgccg acgccgataa cggcaaacgt
     1441 ctcgctgaaa cgcgctgcgt cacctgccac atcgtctcgc cgagcgagcg tcgagacgtg
     1501 accgacgccc caccgtttga agcgatcgca agaaagtttg cttccaaccc ggacacgctc
     1561 gcgttttcaa tcgttgatcc gcatcccagg atgaacgtga cgctgacgcg ccgggaggcg
     1621 caagacgtcg cggcctacat caacacgctc gcaaagtagt cgacgtgcgc atccaatagc
     1681 cgaggcggct catctcgggt caagccaaat ggcaccgacc gcgtggacca gcga
//
LOCUS       DATLJT010000088         3363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4139141,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000088 DATLJT010000000
VERSION     DATLJT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4139141"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VM910_01850"
     CDS             complement(<1..514)
                     /locus_tag="VM910_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61328.1"
                     /translation="MKVPRRQFLHLAAGAAALPVASRFAFAQAYPTRPVRVIVGFIPG
                     GAPDILARLMGQWLSERLGQQFVIDNRPGGGGNIGTQAVVRASADGYTLLMVGAFSAV
                     NATLYDKLNFNLIRDIAPVAGIIRTPLVMVVHPSFPAKTVPEFIAYAKANPRKVNMAS
                     PGNGTMPHVSG"
     gene            1055..1417
                     /locus_tag="VM910_01855"
     CDS             1055..1417
                     /locus_tag="VM910_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61329.1"
                     /translation="MRFEIARRVFVIALSVALATGMAARSVQALAMDVAAPAATAAAT
                     TDTGMPMSGKCDGCAGDEKAMAAACSAVCSGAVAVLPLIVAFGPVPVETIGPSATPAA
                     TGHAFPPDPYPPRPTVLS"
     gene            1542..2966
                     /locus_tag="VM910_01860"
     CDS             1542..2966
                     /locus_tag="VM910_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HVI61330.1"
                     /translation="MSLIIHPAISRRSFLAGVASAAAAASFPSKAGNSSTAEFGLVAK
                     PGRVPLVGASHSSTAVWCYDGRVPGPEVHVRQGLPVRIVVRNELPEDTTVHWHGIRVP
                     NAMDGVPGLTQPPIKPSEQFTYEFTPPDAGTFWYHPHADSLQQLGRGLAGALIVEEQT
                     VLPVDHDILWMLTDWRLTSDAQIASGFGNGMEAAMSGRVGNTVTINGSMSDEVRVRAG
                     ERIRLRLVNSSLARIMALRFEGHRPTVVAIDGQPCDPHQPEGGRVLLGPAMRTDLVLD
                     MQGEPGRSYRVIDDFYEGLSYWLTKLAYEDGAPIRAHPLDAPLSLPRNPLPEPDLSMA
                     ERHELRLQGGMMGGGAMMGMGGMRGIARGASWAINGVSTICDGGGGMPPLLILPRGRT
                     YVMTIRNETAWWHPMHLHGYSFRVLSRNGVPVPHRQSADTVLVPPKKAVEIAFVADNP
                     GDWMLHCHVTDHQVSGMMAVLRVA"
     gene            2993..>3363
                     /locus_tag="VM910_01865"
     CDS             2993..>3363
                     /locus_tag="VM910_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HVI61331.1"
                     /translation="MNRRVFCVATLMALIAPPPALAEPIHATLYKNPQCSCCEGYAAY
                     LRKNGFEVEVKATNDLAEISSKAGVPENLQGCHTMFVDGYVVDGHVPVSIIRKLLSER
                     PAIAGITLPGMPEGSPGMTGS"
BASE COUNT          630 a         1046 c         1053 g          634 t
ORIGIN      
        1 cgccggagac gtgcggcatg gttccgttgc ccggcgacgc catgttgact ttgcggggat
       61 tggccttggc gtaggcgatg aactcgggaa ccgtcttggc cggaaatgat ggatgtacca
      121 ccatgacgag gggtgtgcga atgatgccag cgaccggcgc gatatcgcgg atcaagttga
      181 aattgagctt gtcgtagagc gtcgcgttga ccgcgctgaa agcaccgacc atgagtagcg
      241 tatagccgtc cgccgatgcg cgcacgaccg cctgtgtgcc gatattgccg ccgccgcccg
      301 gcctgttgtc gataacaaat tgctggccga ggcgctccga cagccattga cccattaggc
      361 gcgcgaggat gtcgggtgcg ccgccgggga taaagccaac aatcacgcgc accggccgcg
      421 tcggataggc ttgcgcgaaa gcgaagcgcg atgcgaccgg cagggcggca gcacctgcag
      481 cgagatgcaa aaattgacga cggggaactt tcatggcgtt tcctttccga aacgctcgcg
      541 acgagctagc tgtttccctt tgatagttcg cggccttgag gacgggccac cacttctcga
      601 tctcccagag aacgcggcga taggccgcct tcccgcgatg cgggtcgggc aaatcgcaat
      661 gatccagcac tccgcgccca cgggccgcct cagcggaata ccactgttat gtagacgctg
      721 cggacgcagt tgcacagtcg tgggatgggt tggcaatccc aaccaaattc accgtgaaag
      781 acgccaacgg caccggcgcc gaggtgatgc aacgcatcgc cgggccgatg atcggcggca
      841 tgggaccctc atcgtcattc catcgatcta cgccgtggtc aaaggcgcca cgatccggtc
      901 gccgcatctc gcgcccacgc tccagcagac ctgaccaccg gagcgtactc acagtcgaac
      961 gagccttgtc acaaagctgg caacttctgc aaagggcaat tccgcgttgt gagcacccct
     1021 ggaatgtcaa gcttcgaatt gttaaagtaa agacatgcgg ttcgaaatcg cccgccgcgt
     1081 gttcgtgatt gcgctctcag tggctctagc caccgggatg gcggcgcgtt cggttcaggc
     1141 acttgccatg gacgtcgccg cgccggcggc aaccgctgcc gcgaccacag acaccggcat
     1201 gcctatgtcc ggtaagtgcg atggttgcgc tggtgacgag aaggccatgg cagcggcatg
     1261 ctctgctgtt tgtagtggtg ccgtcgcagt gctccctctc atcgtcgcgt tcggcccggt
     1321 ccccgtcgag accatcggcc cttccgccac gccggccgcg accggccacg cttttccccc
     1381 cgatccctac cctcctagac ccaccgtcct gagctgagac gcgtcgctga ggcgacggcg
     1441 tcttggcgcg cgtgcgacgc ggaagcgtcg caggagaatc cgagttgagt tgcggacgaa
     1501 ctcgtgccgc atctcgttct tccggtacag gacggctgtc gatgtccctc atcattcatc
     1561 cggcgatcag tcgccgttca ttcctcgccg gtgtggcgag cgcagcggca gcggcatcgt
     1621 tcccgagcaa ggccggtaac tcgtcgaccg cggaattcgg cctggttgcg aaaccgggtc
     1681 gggtgccgct cgtgggcgcc tctcattcat ccaccgccgt ctggtgctac gacggacgtg
     1741 ttccgggccc cgaggtccat gtccgccaag gactgccggt tcgcatcgtc gtgcgcaatg
     1801 aactgccgga agacacgact gtgcactggc atggcatacg agtgccaaac gccatggacg
     1861 gcgtgcccgg cctcacgcaa ccgccgataa aaccgagcga gcaattcact tacgagttca
     1921 cgccgccgga cgcgggaacg ttctggtatc acccccatgc cgacagcctt cagcagctcg
     1981 gacgcggctt ggcgggcgca ttgattgtgg aggagcagac ggtcctccca gtcgatcacg
     2041 acattctctg gatgctcacg gattggcgac ttacttccga tgcgcagatc gcatcggggt
     2101 tcggcaatgg catggaagcg gcgatgtccg gccgcgtggg caacaccgtc acgatcaatg
     2161 gctcgatgtc ggacgaagtg cgggttcgtg ccggagagcg catacgtctg cggctcgtca
     2221 acagctccct cgcgcgcatc atggccctcc gtttcgaggg acatcgtccc acggtggttg
     2281 cgatcgacgg ccagccctgt gatccccacc agcccgaagg tggtcgtgtg cttcttggac
     2341 cggcgatgcg caccgatctg gtgctcgaca tgcagggcga gcccgggcgg agctatcggg
     2401 tgatcgatga tttctacgag gggctttcct actggctcac caagctcgcg tatgaggacg
     2461 gagcgccgat ccgcgcgcat ccgctcgatg ctccgctttc cctcccgcgt aatccattgc
     2521 ccgaaccgga tttatccatg gccgagcgcc acgaattgag attgcaaggc ggcatgatgg
     2581 gcggcggcgc catgatggga atgggcggca tgcggggcat agctcgcgga gcaagttggg
     2641 cgatcaacgg cgtgtcgacg atatgcgacg gcggcggcgg aatgcctccg cttttaatac
     2701 ttccccgtgg gcgaacatac gtaatgacca tccgcaatga aaccgcttgg tggcatccga
     2761 tgcatctgca cggctacagc ttccgggtgc tgagccgcaa tggagtgccg gtcccgcacc
     2821 ggcaatcggc cgatacggtt ctggtgccgc cgaaaaaagc agtcgaaatc gcgttcgtcg
     2881 ccgacaatcc cggcgactgg atgctgcatt gccacgtcac ggatcatcag gtttcgggaa
     2941 tgatggctgt actccgcgtc gcgtgaacaa cgaccttcga aggagaatcg agatgaatag
     3001 acgagtgttc tgtgttgcca cgctcatggc gttgatcgct ccgccgccgg ctcttgccga
     3061 gccgatccat gcgaccttgt acaagaatcc gcaatgcagc tgctgcgaag gttacgccgc
     3121 ctatttgcgc aaaaacggct tcgaagtcga ggtgaaggcc accaacgatc tcgccgagat
     3181 cagcagcaag gcgggcgtgc ctgaaaatct ccaaggctgc catacgatgt tcgtggatgg
     3241 gtacgtcgtc gatggtcacg tgccggtgag cataatccgg aagctgctgt ctgagcggcc
     3301 cgcgatcgcc ggcattaccc ttcccggcat gccggagggc tctcccggca tgaccggctc
     3361 gaa
//
LOCUS       DATLJT010000089         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3909489,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000089 DATLJT010000000
VERSION     DATLJT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909489"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VM910_01870"
     CDS             <1..240
                     /locus_tag="VM910_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61332.1"
                     /translation="GKIVQLFAQSAVSWRRRGIESGQRIQRGRFEANAFLAGKRQIDA
                     KIGAAFVQRTRRTIERRDRKLDQAALTILPLAVDS"
     gene            complement(198..1001)
                     /locus_tag="VM910_01875"
     CDS             complement(198..1001)
                     /locus_tag="VM910_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61333.1"
                     /translation="MHFPASLFVIFFAISASAAEQRTTLDEAAQDYVHLVLEIGAHEK
                     GYVDAYFGPSHWRAEAEAHPRVISELKVEADRIQSTLSAMDASAQQPAERRRNAWLKA
                     SVASARSRLDIIEGARFRFRDEATRLFALTPELRPLESYDPVLARIEAFLPGPGSLAE
                     PVEAFRDRYVIPSNRLAAVMDAAIAECRRRTLAHIQLPSNEHFKIEFVTGQSWGAYNW
                     YQGGNQSLIQVNVDLPIFIDRAVQLGCHEGYPGHHVQESTASGNIVSAA"
     gene            complement(1789..>2484)
                     /locus_tag="VM910_01880"
     CDS             complement(1789..>2484)
                     /locus_tag="VM910_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61334.1"
                     /translation="TYDLTIFKLHCGGLTLKIYSKGERVLRIEVIVHNTRELGCGRAL
                     ENFPEIVRRLQALLERFADALSCIDSCFISDRTLERLPTPSRVGKIVVGGIDLNKPRM
                     RWVAEAVIALSASSDGFTASDLAARVRALGQQTLAQYGPTRAAYDLKKFRGQQIVQRI
                     GATRRYEARPAGLKAITALVVLRDKAIKPLLAAAQELRPSRGAHNPTPLDAHYDALRA
                     AMRGVFHELGIAA"
BASE COUNT          493 a          724 c          794 g          473 t
ORIGIN      
        1 ggaaagatcg tgcagctctt cgcgcagagc gccgtaagct ggcgccgtcg cgggatcgag
       61 tccggccaaa ggatacagcg tggtcgcttc gaagcgaatg cgttcctcgc cgggaaacgc
      121 cagatcgacg ccaaaatcgg cgccgccttc gttcagcgca ctcgtcggac catagagcgg
      181 cgcgaccgaa aactcgatca ggccgcgctc acgatattgc cgctcgctgt agattcctga
      241 acatgatggc cgggatagcc ttcgtggcag ccgagctgga ccgcgcggtc gatgaagatg
      301 ggcaggtcga cgttgacttg gatcagactc tgattgccgc cctgatacca attgtaggcg
      361 ccccagcttt ggcctgtcac aaactcaatc ttgaagtgct cgttggacgg caattgtata
      421 tgtgcgagcg tgcgccgacg gcactcggcg atcgcggcgt ccatgacggc ggcgagtcgg
      481 ttgcttggaa tgacgtaccg atcgcgaaac gcttctacgg gctcagctaa cgagcccggt
      541 cccggaagga atgcctcaat gcgcgccagc accggatcat agctttcaag cggacgtagc
      601 tcgggcgtga gcgcgaacag gcgcgtggct tcgtcgcgga aacgaaagcg cgcgccctcg
      661 attatatcga ggcgagaccg cgcactcgca acgcttgcct tcagccaggc gttgcggcgg
      721 cgctccgctg gctgctgcgc gcttgcatcc attgcgctta aggtcgattg gatgcggtcc
      781 gcttcgactt tcagttcgga aatcacgcgc ggatgagcct cggcctcggc tctccaatgc
      841 gatggcccaa aataggcgtc gacatagcct ttttcatgcg cgccgatttc aagcacgagg
      901 tgcacataat cttgcgctgc ttcgtcaagc gtcgtgcgct gctcggcggc gctggcagat
      961 atggcgaaga aaatgacgaa tagcgatgcc ggaaaatgca tcgaaaaatc tccgttcgcg
     1021 cgtaagctcg gtccggcaag ttgggccatc gcgtacgcgt ttcccaagaa tcctactcgg
     1081 ccagacgggt ccggccaata aaaaatttcg ccggaagctc aacaagagtg acgtcggggc
     1141 ggtaaaggcg ttcatgtgta tcgaagaccg gatcctgaca gcgccggcct gaccgccgga
     1201 aggtgccaat tagtttgctg gcagcgcctc gagcacctcc tgcgcctcgc tggcgtcggc
     1261 aaacacttat gggacaatca ttcgcgaagc cggcacccag ccggaatgat gtagctcgac
     1321 gatgaagatg gtcgaacacg acgaattaca tcaaaccgca cactgcgctc cggtgttgct
     1381 gtgaaagtcc gcgacatttg tggtcggcgc gatgcagccg gcacgtatca cgaaggcacc
     1441 aaaccgcgca attgcatggc tggggcgccg ccccccgggc cgcgaggcgt catccagaaa
     1501 gcgcgtcggg atgcaggggc gatgaagccg tcggaccccg taaccatggc cccggttgcc
     1561 ggttcgccac cctccatggg gaactgccgg agtgtcgcgg aattgatccc gttggaggtt
     1621 cgccgcgcat ggaattcctt catggcgtca ggtggctcac gggcctaagc gccctgctgc
     1681 gagcccgcgt gtcctgacac ggaggatgct cggtagcgtg ggcgaggaca tcggaaaggc
     1741 gacctttagg cgcttaggcg cggtgcttcg aaaaacaatt ttcgatcttt atgcagctat
     1801 accgagttca tggaacactc ctcgcatggc tgctcggagc gcgtcgtagt gcgcgtcgag
     1861 cggtgtcggg ttgtgggcgc cgcggcttgg tctgagttcc tgggcggcag cgagcagcgg
     1921 tttaatggcc ttgtctctga gaacaaccag ggcggttatc gccttgaggc ctgctggcct
     1981 ggcctcatag cgacgtgtcg cgccgatgcg ctgcacgatc tgctgaccgc ggaacttctt
     2041 taggtcgtag gccgcccggg tgggaccata ttgagcgagt gtctgctggc cgagcgcacg
     2101 gacccgtgcc gcgagatcgg aagcagtgaa gccgtcggag gaggcggaca aggcaatgac
     2161 tgcttcggca acccagcgca tgcgcggctt gttgagatcg atgccgccga ctacgatctt
     2221 accgacacgg gatggcgtcg gcagacgctc aagtgtccga tccgagatga agcatgagtc
     2281 gatgcagctg agggcatcgg caaatcgctc cagcagagcc tgcagtcggc ggacgatctc
     2341 ggggaagttc tccagcgcgc gaccgcagcc cagctcgcgc gtgttatgga cgatgacctc
     2401 gatgcgaagc acgcgttcgc ctttgctata gatcttcaga gtcaagccgc cacagtgcag
     2461 cttgaagatc gtcagatcat aggt
//
LOCUS       DATLJT010000090         1951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3909619,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000090 DATLJT010000000
VERSION     DATLJT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909619"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1313
                     /locus_tag="VM910_01885"
     CDS             168..1313
                     /locus_tag="VM910_01885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI61335.1"
                     /translation="MTAVASIALAVWLYLLLARGAFWRCSERDDWGPAELPGWPRVAA
                     VVPARNEADCIGDSIGSLLAQDYGGEWTVILVDDDSSDGTAEIARRVANGDARLRVAR
                     SRGLPAGWTGKLWAAKQGVDAASALPQPPDYLLLTDADIVHAPDSVSRLVAHAERQHL
                     VLTSLMVKLHCESFAERATIPAFIFFFQMLYPFSCVNRPESAVAAAAGGCMLVRADAL
                     REAGGIEAVRAALIDDCALAKALKAQGPIWLGLTERVHSVRRYPAGADIRRMVARSAY
                     AQLRYSPLLLLAMMALMALTFLVSPLATIFGSGAARWIGAGGWALMFVAFQPTLRFYR
                     LSPLWGLALPAIALQYMLYTLDSAHQYVRGKGGSWKGRVQANVSERR"
     gene            1310..>1951
                     /locus_tag="VM910_01890"
     CDS             1310..>1951
                     /locus_tag="VM910_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HVI61336.1"
                     /translation="MITPAEARSGKGHRDENFPVASRLIHPRHRAPILAFYEFVRTAD
                     DIADHSALAPQEKLALMDRLEATLLGQSNDDPVGVALRSQLAARQLSPRHAQDLLAAF
                     RMDVTKLRYRDWDDLIGYCTYSAMPVGRFVLDVHGESRATWPANDALCAALQIINHLQ
                     DCGKDYRQLNRVYIPLDVFAETGASVDELGAGHASARLLQCIHQLASRTGALLR"
BASE COUNT          314 a          625 c          618 g          394 t
ORIGIN      
        1 tccacacagc ccgcatgcgg gccgcctgac aatctagcaa gggcgacccg cccgtccaaa
       61 cttttcctaa cccaattcat tgaatatgtt gaattgtttt tgctgacccg gtccgcgggg
      121 cctgggatcg gccgctatgc ccgagatact acggaggctt tgctcgcgtg acagctgttg
      181 cttcgattgc gcttgcggtc tggctctatc tgctgctcgc ccgcggcgcc ttttggcgct
      241 gcagcgaacg cgacgattgg gggccggcag aacttccggg atggccgcgg gtcgcggccg
      301 tggtgccggc tcgcaacgag gcggactgca tcggtgacag catcggatca ttgctcgcgc
      361 aggactatgg cggtgagtgg acggtcatcc tggtcgacga cgatagcagc gatggcaccg
      421 ccgaaattgc gcgccgggtt gcgaacggcg acgcgcgact gcgcgtggcg aggagccgcg
      481 gcttgccggc aggatggacg ggcaagctct gggcagcaaa gcagggggtc gatgccgcct
      541 cggcgctgcc gcagccgccg gattatctgc tgctcacaga cgccgatatc gtgcatgcgc
      601 ccgattccgt cagccgtctg gtcgcgcacg ccgagcggca gcacctcgtg ctgacgtccc
      661 tcatggtcaa gctgcactgc gaaagcttcg ccgagcgcgc aactattccg gcattcatct
      721 tcttcttcca gatgctctat ccgttctcct gcgtgaaccg accggagagc gcagtcgcgg
      781 cggccgccgg cggctgcatg ctggtccgcg ctgacgccct tcgcgaggcc ggcgggatcg
      841 aggccgtacg cgcggctctc atcgatgact gcgccttggc gaaagccctc aaggcccagg
      901 gaccgatctg gctcggattg accgaacgcg tgcacagcgt ccgtcgttat cccgccggtg
      961 ccgatatccg ccgcatggtg gcgcgttcgg cgtatgcgca gctgcgctac tcgccgcttc
     1021 ttctgttggc aatgatggcg ctgatggctt tgacttttct cgtatctccg ttggcgacga
     1081 ttttcggcag cggcgcggcg cgatggattg gagctggagg ctgggcgctg atgttcgtcg
     1141 cctttcagcc gacgttgcgg ttctaccggc tgtcgccctt atggggtctc gcgctacctg
     1201 ctattgcttt gcagtacatg ctctacaccc tcgattcggc acatcaatat gtgcgaggaa
     1261 agggcggaag ctggaagggc cgggtgcagg ccaacgtctc ggagcggcga tgatcacccc
     1321 cgccgaagcg cgatcaggca aaggccaccg ggacgagaat tttccggtgg catcacggct
     1381 gattcacccg cgccatcggg cgccgatcct cgcattctac gaattcgtac gtaccgccga
     1441 cgatatcgcc gaccactccg cgcttgcgcc gcaggaaaag cttgcgctga tggaccgact
     1501 cgaagccacc ttgcttggcc aaagcaacga cgatccggtc ggcgtcgctt tgcgctcaca
     1561 gctcgcggca cgccagctct cgccgcgtca cgcgcaagat ttgctcgctg cgtttcgaat
     1621 ggatgtgacc aagctgcgtt accgcgattg ggacgatctg atcggttatt gcacgtattc
     1681 cgccatgccg gtggggcgtt tcgtgctcga cgttcacggc gagagtcgtg ccacctggcc
     1741 cgccaatgat gcgctctgcg cggcgctgca aatcatcaat cacctgcagg attgcggaaa
     1801 ggattaccgt cagctcaacc gcgtctacat tccgcttgac gttttcgccg aaaccggcgc
     1861 gagcgtggac gaactcggcg ctggtcacgc gtccgcgcgc ctgctccaat gtatccacca
     1921 gctggcgtct cgcactggcg cgctgctgcg c
//
LOCUS       DATLJT010000091        30674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_692720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000091 DATLJT010000000
VERSION     DATLJT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 30674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30674
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..493)
                     /locus_tag="VM910_01895"
     CDS             complement(365..493)
                     /locus_tag="VM910_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61337.1"
                     /translation="MSKRLGSRYRSGRSKDWLKFKDPAAPAVKREAEEDWGRERWR"
     gene            446..823
                     /locus_tag="VM910_01900"
     CDS             446..823
                     /locus_tag="VM910_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HVI61338.1"
                     /translation="MLRSTGAVARAEPLRHDASTDTRSELGARFDRVLAVYSNAVLAL
                     RGRVAWAHDWVSDATLLPVFQSLPGDSFIVNGATLPKNSALTSAGAELRVANGVTLLA
                     KFDGEFASHSSTYAGSGAIRYRW"
     gene            2068..3552
                     /locus_tag="VM910_01905"
     CDS             2068..3552
                     /locus_tag="VM910_01905"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HVI61339.1"
                     /translation="MIEAETKRALAQQRQEQEAARRREQREKEKARQEKVTAKEKRQD
                     DDRQDRKARQDARDQEKARKETERLDRERKKREAVFAEIADLPKLTHEARLKEAAIRL
                     GEDFELLLEEFEVFLAARTIPEDLEPWPEPVNMAKLLGEVETKFCRYVVANKAIVTAS
                     VLYSLFTYVVEIARHAPKLVYTFPTRDAGKSVAQEVLRWMVLRPYAAVEATGAAVYRI
                     IDRLRPTLCLDEADTLFARRGSVLAHILNNCWSNNKRMIPRAGSRGKSVDEYDVYGAQ
                     IISMRGLNMPDTTQSRSIICLIWPKLPTEVVEEFTYQDDDEFKAIRRKLLRFAIDNAA
                     TLRDAEPQFPPGFNNRIRTNWKMLLAIAELAGGEWPKRVRKAALELEADRDEPSEDIR
                     LFQAVKDVWDRAEKERTSESLCAALAAHPSGEWVDITPHKFAAKVRPYGIKPIHGLHP
                     TGRSDLTRGGYRYAQFENVWARLLQKPSRDSHTRTPSGRRRRKT"
     gene            3633..4043
                     /locus_tag="VM910_01910"
     CDS             3633..4043
                     /locus_tag="VM910_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61340.1"
                     /translation="MTSSQDDDDEERAQRRPPSHVFFPPDTPLLKYLVPRILRASLDG
                     VGEGGDHEAEERAMACFIVLISHLAGITDDEVRNRTVDELSKTAALQVSLIRQLVSKR
                     SSAAAARGMSLREAYEEAARAGGFEPLPDEPSEH"
     gene            4116..4322
                     /locus_tag="VM910_01915"
     CDS             4116..4322
                     /locus_tag="VM910_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61341.1"
                     /translation="MASTEFALGAIDRRAGRGYRSDYTTWSGNQQWNYERGRAWATTA
                     PRSVPLKRNGKLTPAALRFAGDII"
     gene            4375..4614
                     /locus_tag="VM910_01920"
     CDS             4375..4614
                     /locus_tag="VM910_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61342.1"
                     /translation="MRQVAKRSGKVRAKARKQNARSAVREITYAPTEKLIPVLKAMLD
                     DPLRSTKYDEETLEKAETILRMLRDLEKRGFFRPS"
     gene            4918..5556
                     /locus_tag="VM910_01925"
     CDS             4918..5556
                     /locus_tag="VM910_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVI61343.1"
                     /translation="MPLWLSRKLARRDPAPPWRQVFFLAFVGIRGVVSLAAALAIPLT
                     TLAGVPFPYRDLILFVTFGVIVVTLVGQGLVLPGVVRCVGLGSHAAEEREREHAAELS
                     ARAEALNVVQNRLDRLAAEGRIAPEVLAILRARHHDRVGRLPRSPSDGFETASAVAEL
                     RSDLIAAEREFIYQLFREGKITDEARRRIERELDLEEAGIACKKEGGVEPPL"
     gene            complement(6917..7111)
                     /locus_tag="VM910_01930"
     CDS             complement(6917..7111)
                     /locus_tag="VM910_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61344.1"
                     /translation="MNMISVPYGLDLNDSVIYAIEKHSMPEMKLRIEQAIRTFEREIT
                     ESSDNRASSLFRYTRTSHQA"
     gene            7316..8146
                     /locus_tag="VM910_01935"
     CDS             7316..8146
                     /locus_tag="VM910_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI61345.1"
                     /translation="MPAFICNTCGTQYAPTEKPPASCPICEDERQYVTTAGQSWTTLD
                     VLAKRNMNAIRELEPGLIALLTFPHFGIGQRALFLRTPHGNVLWDCIALVDRATIEMI
                     NAMGGIKAIAISHPHYYTTMVEWSEAFGGVPIHLHEADRKWIMRHSPSIALWKGQTKE
                     LLPGLTIICSGGHYPGGTVLHWAAGAGGKGALLSGDIVQVVQDNKSVSFMWSFPNVIP
                     LSAPRVEGVVYSLKAYNFDRIHGAFIDRTVWSDGKGVLERSAARYLKIIRGDGTYELQ
                     "
     gene            8811..9608
                     /locus_tag="VM910_01940"
     CDS             8811..9608
                     /locus_tag="VM910_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HVI61346.1"
                     /translation="MIVRYESDGSIVMITQNDHAQLSGLFAAHWGNGRFEKPRPYGSL
                     VRAAMFHDRGWIRYETSPQLNPETGKTPNYREVPNDRMQLEAFEWAGDWLSAIDPYAG
                     LMITKHRSGLWQGRYGVITHPPAIQRGALPPEIQAFIAKSEAKQKVAAGKLDAKEMAI
                     NYNLLQVWDMMSLYICSTEVLKPDRIEPVPVAYSGDAGVKMTLTPLKANTIVLDPYPF
                     DQCSLTANVIFRRLRQTRFKDSAELQTVYFKTAPQIASFTLVSPALN"
     gene            9992..10810
                     /locus_tag="VM910_01945"
     CDS             9992..10810
                     /locus_tag="VM910_01945"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61347.1"
                     /translation="MKPMRAADSLDTSTNRAPALILARGLSKTYITASGEVTALKSLD
                     FEIYDGEFVSVIGQSGCGKSTLLKVLAGLLPCTTGSVELGGKPLRGPTPEAAVVFQSP
                     VLLPWRTLVDNVLLPIEFRKLPTASYRQSAADLLAMVGLTDFAQRYPFELSGGMQQRA
                     ALVRALVQDPRLLLMDEPFGALDAMTREQMNLELLRIWSQSRKTVIFITHSIAEAIFL
                     SDRVIAMTARPGTIADVITIDLPRPRALSSINTERFGRYAAKLRSLLDAQGGMF"
     gene            10810..11688
                     /locus_tag="VM910_01950"
     CDS             10810..11688
                     /locus_tag="VM910_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61348.1"
                     /translation="MMVQAVDSRSVDSGAQAREPRPGVLRRYPQFTISPTILVALLVA
                     WWLATDVLNTPGYIVPPPQDVLRAAVGGLSRSPWDKAGYWYHAAITVWEAVLGFAIGS
                     ALGACLGLVLSHWPILGKSWYPYIVGFQSLPKVALAPLMVVWFGFGLEGKVFITAIIT
                     FFPVLVNMMAGYQAVEPERIELARSCNASELDLLTKIIIPSCLPFLFAGLGVASVLSI
                     LGAVVGEFVGASAGLGMLLMQYNQAMEISPMFAVILLLAVLGFLMNYAVGLVERRYCF
                     WAQRANAMARTDWDRP"
     gene            11704..12789
                     /locus_tag="VM910_01955"
     CDS             11704..12789
                     /locus_tag="VM910_01955"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61349.1"
                     /translation="MLTRLCHVTLALTVATGASGTHATDVAAQSLKKMTLVQMHPIMG
                     IGEEVFLYAVPKRLGYFAAEGLNVDIQNSQTGMISAQVLQSNNAQVGTTAAEAVMAVR
                     EQSGDLISFFNLKRNAGTFLVVLKDSPIQKLEDLKGKTVGAPSFGAGGGLALKQNLSE
                     IGITPDQYIPIATGAGPSAIAALHTGKIDALVMWDAMLGAAENTGLALRVVNIPLEDR
                     MVGTTLATKKSFADANAKELAGYCRAMTKGLVFTMTNRAAAIRIFWDEFPTTKPANLD
                     DATALKNSVHIMDRFLEMALQGQPEGSRLGEFIATNWQNTHAAYVKLGTLKGTEAATD
                     SYTEHFLAACNDFERATVVAQAKSMNQ"
     gene            13785..14606
                     /locus_tag="VM910_01960"
     CDS             13785..14606
                     /locus_tag="VM910_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI61350.1"
                     /translation="MTLRTWFRMLGITVLTFSLPEVALAASSLDKVKQSGVIKACLAQ
                     QQPDNYKDPRSGQWTGVMVDLLNELSKWMKVKIDIAEVGWDVAVLSLKQGTCDLFASS
                     IVYTAPRAMEIAFVTPFGAKGDNIVIDKKNPKNIKVHADLNSPNITIIAELGTREHDN
                     AQRFFPKAHILAVKVPSTVQVIDWVKRGDADAAVLPTITARWWLNVPENAGWAAMGFP
                     DNDFGNAPNGWAVRQGDPDWLDFLNSFSGWVTANGMAKQLYDEYLERTNPFSKKD"
     gene            14658..15350
                     /locus_tag="VM910_01965"
     CDS             14658..15350
                     /locus_tag="VM910_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI61351.1"
                     /translation="MSFGRFYRLIADNADVIGFGIWVTIQLAIVAMLIAVILGLIACL
                     GSLSRARILRFPSMLFVEFGRNTPILVQLIWVHFAWPEILGIKFSAWTSAVIALSLQS
                     SGYLAEEFRAGIESIDKGQIEASQALGMTYQRLMRRIVVPQALMRMIPGILNQFVTCF
                     KSTSIVSVIAVPDLMYQAGLIVSMTFLPMPVYSFVAFLYFLMVMMIALGVRFATRRLP
                     QFGYFNQRHGGE"
     gene            15350..16129
                     /locus_tag="VM910_01970"
     CDS             15350..16129
                     /locus_tag="VM910_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVI61352.1"
                     /translation="MQLGHPVKPLVRLSAVWKRFGKLDVLRGIDLDVNDGATIAIIGP
                     SGSGKSTLVRCLNHLEPIQDGAIEIDGFLITPRGVEKSGRLLGQREVAKFRTLLGMVF
                     QQFNLFPHLTVLGNILEAPTGVLGRDRATVTEEAMDLLAKVNLADKANVYPARLSGGQ
                     QQRAAIVRSLIMHPKIMLFDEPTSALDPELIGEVLKVIGELAIKGRTSIIVTHELAFA
                     RDIASKIAFMDAGLIVEEGPPDKLLTSPKLDRTRAFIARIL"
     gene            16174..17304
                     /locus_tag="VM910_01975"
     CDS             16174..17304
                     /locus_tag="VM910_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVI61353.1"
                     /translation="MKKSHDILINLNRLNQKMDEYGLAGIVARAGVNYTYLSGVSYRG
                     TLARHLDLTDSPRGTFLVWPRHGEPRIVLNAFAEGLTRRDSWVEIIDVYEGYSELPVE
                     RLAKVISDMGLADETVGFEQNFVSAADWGMLRSRLPRMRMVDSTTAMDEVRAIKTAGE
                     VSLLKRAADILDDAYTAVLPTVRPGMRERDVHARLVAECLVQGCEFVHGILNSQRNTI
                     PYAGESDFAFADRDAIRSDYVAYLMGYPGHQSRCAVVGKPTAEQRQQYRVVRDIYRAS
                     ADQCRVGKTADEIYRNVVDRFSAAGMQYTTLLAGHSVGCWWHQQEPVIARGNLRRLED
                     GMVIAMEPHVNHWHIQDMFIVREEGPELLSDKFNTDELFSCG"
     gene            complement(17306..18586)
                     /locus_tag="VM910_01980"
     CDS             complement(17306..18586)
                     /locus_tag="VM910_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI61354.1"
                     /translation="MQKRLDSGPAISTVNERRIFELEQRSIRIPSSTFEEGEIADLYA
                     STMADIGLDVEMMEVIHPQDPAKKSRQPIGRLRGTGEGPTLMLNGHMDPGAEMTGWTV
                     DPLGAKFEDGWIWGIGAHDDKGGCVAAISAVEAIVRSGTRLRGDVIVTPVIAHKLGGA
                     GTRALLDRGVKADLCINMEHSNNTIANVCVGVVMARLVCTAPELFFRYSAEARAAYMN
                     PIEQQSEIVRRIGPSLKPIPHGGWMTFKSHPELPGFPTHTFDVIHKEHYYFKNYTGRS
                     SRECELTLQFRTVPGQTTASIRKDLTRLLAAIKREHPAFNCTLEIPARGTESGWNQAP
                     MECPSDHALVRALAEGQTIASGEQSYIGADGRLGNVGDGNIIQEKLGIPTVQYGPGDI
                     RIYKEWPTPDERVLLADLVTAAKAVAHAAVCLCG"
     gene            complement(18871..19320)
                     /locus_tag="VM910_01985"
     CDS             complement(18871..19320)
                     /locus_tag="VM910_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HVI61355.1"
                     /translation="MAHAIEIGRAGLRTKDARPFAAVVVRQGKIVGEGRSQQKTLLDP
                     TAHGEIVAIRDACRRLGTTDLSGCELYTTCEPCLLCVSAIWWAKISKMYYAHTQEDCE
                     RIGFSTKALVDEVRRPPASRKLPSERICVAEAEMLFQEWAASPNFEP"
     gene            19760..20974
                     /locus_tag="VM910_01990"
     CDS             19760..20974
                     /locus_tag="VM910_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61356.1"
                     /translation="MPSLDICSATRPAVALAAGIGLLTLAFGAPAQENGITPSEISIG
                     AIGALTGPLAFIGTPGRDSMMLAFDEINAKGGVCGRKLRLDFEHASSPAESVAAVKKL
                     VEQDKVFILVLASGSTGAAAAADYVRQVGVPTYNLYGSTPIIREPFAKNVFHGAVVPV
                     QVSAAALISMFYDGEYTPKKFGILAGTYAFPQATLKAAQDILGQKGATFTVEQFDIGA
                     RDFTSQLVSLQRQSVDSVLVLGSFSEAGFAIKQAREMGMANVRWVVDGSAVSTAIIPI
                     LGNADGIRGYYNAPAFPGQTPATREFEARLKKQLGSMPQGRPSVYDLIGYGSGYVVAE
                     AIGATNCQLTRDNLINAWSNLKDAGPTRLDGLDITFPESYSPTDHQGVYRIGAAIVKD
                     GQWQVYRVIDKP"
     gene            20982..21860
                     /locus_tag="VM910_01995"
     CDS             20982..21860
                     /locus_tag="VM910_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61357.1"
                     /translation="MLTELGFVVLAGIKFGALYALAALGLVVVHKATKTVNFAHGAFA
                     MLGAFASFLIVVNWHWPYWAAYLLIPWAIGLIAAAMESAILRPLRRADMFTVVIATVF
                     LGIAISEGFRLTYNTELLAVPSLFAGPPFIVAGVLITRDTLWVIAGALAAALACIALF
                     SYGRFGRGMRAMAANIRGAQLCGYSIDRVYMLAWFLGGGLAGLTGVLIAPVTGVSIDL
                     SIAIITAGFVAGVIGGFDSPKGAIVGGLLLGIAESVSAAYVSSAMKNCVSFLLLFLVL
                     MWRPQGMFPERSGRSV"
     gene            21853..23643
                     /locus_tag="VM910_02000"
     CDS             21853..23643
                     /locus_tag="VM910_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI61358.1"
                     /translation="MSDRRPDLAALGPALLIILCAAVGLRLDSYPQYVIAVAIISMLV
                     GTALVVLVGLARCITLASGSIMAIGAYVSTLAVAQLRVPYLGTLALAILAGGAGGWVI
                     AFLGTRFRGHNLAMVTLVFQSVSIILIRESRWLTGGAEGIHVPAPEIFGLAIATDRAF
                     LIMIGIAVAVMTLIVTILVRGPFGKNLQAASGNEIAAEAFGVSVRGYLTAAFVVSSIA
                     IAFAGALLAPRVRILDPDSFGVLESIFMLAYPIVGGMHAVWGGLIGGGGLRILPELLR
                     PVADYQELFFATLVIFVVMFFPGGLTELIRRVVASKVKPTPPALAPVPAVPAAPTDRD
                     ETGPGGHELLLVESVDKHFDALHAVNDASLRVPARAIHGLIGPNGAGKTSLFNILSGF
                     LAADAGRVTFAGTSLLELRPRDRIRLGITRTFQNVAVFGQLSCLDNVIIGLGRNAVLH
                     AMGASFDHFARGAATRGAQRAALAALESVGLAAQAYSRAGSLSLGDQRRLELARAIVY
                     HPRLILLDEPVSGLAESETEDLRELLLRINAERGIAMLVVEHNIPFVSRLCRSLSVMG
                     GGRIVAEGKPADVISLPVVQQLYFGEQATS"
     gene            23640..24359
                     /locus_tag="VM910_02005"
     CDS             23640..24359
                     /locus_tag="VM910_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61359.1"
                     /translation="MIALRATDLTVAYGPVVAVRDLSFSLESGEALVLLGANGAGKTS
                     AVEAIAGLLPKRRGNVEFLGRDISRLSASAIVRSGLALVPQWRELFPAFTVEETLIAA
                     TTAAIGRAPTPLNSVYELFPVLQERRRQTAGSLSGGEQQMLAIARALVTNPKLLLLDE
                     PSAGLSLGIARTLIGAINRIREGGVAILLVEQNMEIARAIGERCCMLRAGRLAWAGSM
                     QEAMARNEAERVYFGAHGRVC"
     gene            24579..25592
                     /locus_tag="VM910_02010"
     CDS             24579..25592
                     /locus_tag="VM910_02010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HVI61360.1"
                     /translation="MTALFSVGFPTGMEGLTYPIPFSNPEDLVRIAQHAEALGYHSVW
                     GNDHMTTQHYVRAEFPVPPRFWEPLVTYAYLAAHTKRLRFGTGVLVLPMRRDIVVTAK
                     QIATLDHFSNGRVEIGVGIGAYREEFEALNPQARVHRGDMVVEGVTALRLLFSQRAAS
                     FDGKYYKFRDVELFPKPKQQRLPIYFGGNNPAHLRRTAASGDGWIPAAMPQPTLRRMV
                     AELREMTEAAGRKFSELQIAPQYIVHLGRNQEQAVARFRQSQMFKHLESLKRSTLRGQ
                     ASVNPEDVNLVGSIDIVIARATALREAGVTHFLGLFFAANSVPELLDQMQMFAEEVRP
                     KIA"
     gene            25873..27114
                     /locus_tag="VM910_02015"
     CDS             25873..27114
                     /locus_tag="VM910_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVI61361.1"
                     /translation="MAADASAAIGQRSLGDPSLLFPLKPPLLRGCPRTSMAEMQYPLE
                     IDFDYAKVSRDIFHQPPLSGLQRWAPLLPPLVPELSLGEGGTALIPSHRIARWAGLDG
                     PIWLKDESRNPTWSHKDRLNYCIVSTAVANGARGIAIASTGNHGASAAAYARRAGLRC
                     VVISTPDAQRAFEHFQTALGSDFLLVSREERWPTLNRVVDETGFMPASNLTRFHTGNP
                     FGPEGYKTIAYEIFSQLGGRVPGTVVLPTGYGELLFGVAKGFRELKSFGLSDTVPQML
                     SAEPAVRAPLARAMSRNEAAAEVSGPPSRAAGIACTVSSYRAVVALRGSHGRPLTFHE
                     DVVAEAASELAHEGLWQEYSGVAGLAALHEARARGEVFAEPVVTVLTSTGLKEMPAPS
                     VVSYATALPSLDSVMVSLLRA"
     gene            27126..28106
                     /locus_tag="VM910_02020"
     CDS             27126..28106
                     /locus_tag="VM910_02020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI61362.1"
                     /translation="MQIGVSVDGNEPPSLLRALTEAAEAAGAANLWLATHLFNREPIA
                     CATAVLAQTRRLGVVLMAMSPYTVHPVYAAMAAATLDELFPGRVQLCFGVGAPGALEA
                     VGITAPHPMETLREAMAVARLLLSGEPVTFSGRRYRVSGRLAMGAHRLPVWLAASGPR
                     MLELAGETADGVVISAGTAPAFIGWCLQSVRRGEERGTRAVRKAALVACSVDADAHRA
                     HERVRRRLAYILRGSHHARNLELAGTRLDQSALAEAFARESWQRVDELTGDDVVVRHC
                     ASGTPEQAMTMLQAYRDAGLDEIVVYGMQERQQLEDVLALMCPQRASVGR"
     gene            28112..29290
                     /locus_tag="VM910_02025"
     CDS             28112..29290
                     /locus_tag="VM910_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVI61363.1"
                     /translation="MLVRDDLRAIAEKVRSKVFTACDQDADVIISTDPTHVGYLCGYR
                     SILFDLMRDYRAAAIVTRDKAVLVTGASDVAAALEVLCDPACIYRYGLFFFESSNGAG
                     VDLSALPKPESTFVDALCAAVAATVKPRQRVGLDCASAWELQELKNLAGPRSFDARPA
                     ILKARRTKLPEEIEKIAHAATITERGLEKALARGKAGVSELDLSTIIAGEMRAGGGVP
                     RFVVVTSGERSALADAYATPAKLRSGDLVRFDIGCTVDGYWADMARTAVVGEPTREQQ
                     QRYDALLAGEEAQRALARSGVSAGNLFKVAVERVRQGALPAYRRSNCGHGIGIDVHEF
                     PALSAANEGVALEDGMTLCVETPYYQISWGGMMVEDMIVVGPTGNECLTHLPRELRRL
                     "
BASE COUNT         5928 a         9168 c         9352 g         6226 t
ORIGIN      
        1 gtggcctggc agggcgacac caaatctcga tagggcaggc gcaagggcac tgtatgtggc
       61 ttctccatta cccgcatgta tggcgcgggg ctctgggata gccacatagg caaattcatt
      121 cgacgtcgaa tcgaccggag cgttccacga tccgccaccc ttacttcggt ccgcgccgac
      181 acgaacgagt agacaccgca cggcatcacc ccctaccgta gtttttgacg aagtgccgat
      241 caattcaagc caacgccaga gtgtagtcct cgacgcctgg ccaaatgctc aacgatgctc
      301 gcgccgtcag cggtccatgt gtcgatgcgg aacgtgaaat gcgtccgcgt cttctccggc
      361 ttcttcaccg ccaccgctcc cggccccaat cttcctcggc ctcgcgcttt accgccggcg
      421 ccgccgggtc cttgaacttc agccaatcct tcgatcgacc ggagcggtag cgcgagccga
      481 gccgcttcga cacgatgcct cgaccgatac taggagcgag ctgggcgcgc gcttcgaccg
      541 ggtgctggcg gtctattcca acgcggtgct ggcgctgcgc gggcgcgtcg cctgggcgca
      601 cgactgggtg agcgatgcga cgctcctgcc ggtgttccag tcgctgccgg gtgacagctt
      661 catcgtcaac ggcgcgacgc tgccgaaaaa ttccgcgctc acctccgccg gcgcggagct
      721 tcgcgtcgcc aatggcgtga cgctgcttgc caagttcgac ggcgaatttg cctcccactc
      781 gtccacctac gcagggtcgg gagcgattcg gtacaggtgg taaatgccag agtgccattc
      841 acgtctgctt ttcatcaatc gcgtcacacc ggggcgccgt ttggtttggt tcgtcccggc
      901 ccatcgatgg tgacgacgcg ccgtctgtag cataggttgt ggcatggcac tcgatcccgc
      961 ggttcttaaa gcgcgcatca gcttgcgagt ttgtcagttg aaagaacttg ctgcgtggac
     1021 cgcagtgaat tggcacgggc gtgcggagcg ggagcagccg ccacaacctc ctggacgccg
     1081 cgacgaagat gacgagtgaa ctctcccatg ttgttggtct ttgaccgcgt cctttcctgg
     1141 tgctgtgtcc tggtgcccgc acgcgtccct tcaatcgctt tccgttttct ctccaggttt
     1201 tcctaggtca tccggtaagg cccatctcga aataacgcac cggcgggctc cgcatttagt
     1261 tgagcgcgtc gcgatttcaa ctttaattta aaaaatcgac catctgagct gtttcggcta
     1321 ctttcgccta caacgtcacc ggttaggtga tgacaggccg agcgcggccg actaagatcg
     1381 cagcgtgttc tcagagaatg cccgcgcagt cgaaagctct ccggagactc tggcgggttc
     1441 gaccctaaac gatgtgccgc gagaacacct gagccggatg cacaacgcct gcgggcgttg
     1501 attcccagct tcagcataag cgctgaaagt gcccaacccg ccgtgtgacc ggcgggccag
     1561 gacttgcaac agccgctgtg ttggagcgac cgtggccaaa tctccccata gcaaaaaact
     1621 ttgtaacgcg cacgccgtgt tgcgccgctt gttctttgct tcgcgcgccg atacggagcg
     1681 caaccagctt gcagcactca agcattgtgc ctcgtcatga ctgacgacag cgctagcaac
     1741 ggcaacaatg aggtcagccg cgcccctata gagcgaacgg taacgtggcc ggtgaaagac
     1801 ggtgacgctc tcatcggcac cgtgcggctt tcgaagggcg tgcatgaatg cgtgctgcct
     1861 gatgggacag tcgtcgagac gttcaagagc cataagtttg cattcgagtt tttgcagcag
     1921 caaggacgac cgatctcgcc ggaggagcag gcattaaggc tcaagactga tgtcgaagat
     1981 ctgcgcctca agtctccgac cgagcaaata tattggctac cggactatgc taagcgacac
     2041 ggcatcgccg agaccaagct cgagcagatg atcgaagccg agacgaaaag ggcactcgca
     2101 cagcagcggc aggaacagga ggcggcccgt cgacgtgaac agcgcgaaaa ggagaaagcc
     2161 cgccaagaga aagtaacagc caaagaaaaa cgccaagacg acgatcgtca agatcgcaaa
     2221 gcccgccagg atgcacgcga tcaagaaaaa gcgcgcaagg aaactgagcg cctcgaccgc
     2281 gagcggaaga aacgcgaggc cgtttttgcc gagatcgccg atctcccgaa attgacacat
     2341 gaagcgcgcc tcaaggaggc ggctatccgc ctcggcgagg actttgaact tttgctggag
     2401 gagttcgagg ttttccttgc tgcgcgcacg atcccggagg acctggagcc gtggcccgag
     2461 ccggtgaaca tggccaaatt gctcggcgag gtcgagacga agttctgtcg ctatgtggtc
     2521 gcgaacaagg ccatcgtcac ggcgtctgtg ctgtattcgc tcttcaccta cgtggtcgag
     2581 atcgctcggc acgcccccaa gctggtctac acgtttccga cacgtgacgc gggcaagagc
     2641 gtagcgcagg aggtcctacg ctggatggtg ctgcgcccgt atgcggcggt cgaggcgact
     2701 ggcgccgccg tttaccgcat catcgaccgc ctgcggccga cgttgtgcct cgacgaggcg
     2761 gacacactgt ttgcgcgccg tggcagtgtg ctggcgcaca tcctcaataa ctgctggagc
     2821 aacaacaagc ggatgattcc tcgtgccgga tcgcgcggta agtcagtcga tgagtatgac
     2881 gtttatggcg cgcagataat ttccatgcgt gggctgaaca tgcccgacac gacgcagagt
     2941 cgttctatca tttgtttgat atggccgaag ctcccgaccg aggtggtgga agagttcacc
     3001 taccaggacg atgacgagtt caaagccatt cgccgtaaac tgctccgctt tgcaatcgac
     3061 aatgcagcga ccctgcgcga tgccgagccg caattcccgc ccggattcaa caaccgcatt
     3121 cgcacgaact ggaaaatgtt gctggcaatt gccgagcttg ccggcggcga gtggcccaag
     3181 cgggtgcgga aagccgcact ggaactggaa gccgatcgcg acgagccgag cgaggacatt
     3241 cggcttttcc aggcagtgaa ggatgtctgg gaccgcgcag aaaaggaacg gacctcggag
     3301 agcttgtgcg cggcgctggc ggcgcaccca tcgggagaat gggtcgatat cactccgcac
     3361 aagtttgctg caaaggtgag gccgtacggg atcaagccca ttcacggttt gcacccgact
     3421 gggcgttcgg acctgacgcg gggcggttac cggtatgcgc aattcgaaaa cgtgtgggcg
     3481 cggttgctgc aaaaaccgtc ccgcgactcg cacactcgca cacccagcgg taggcgtcgg
     3541 cgcaaaacgt agtgtgcgag tgtgcgagtc ctacgacgat tttttcgggg gcgagtgacc
     3601 ccccgtcggc cataagtcat caagaggaaa ctatgaccag tagccaagac gacgatgacg
     3661 aagagcgcgc ccaacgccgg ccacccagcc atgttttctt tccgccggat acaccgcttc
     3721 tgaaatatct cgtgccgcgg attttgcgcg catcgttgga cggggtcggc gaaggcggcg
     3781 atcatgaagc cgaagagcgc gccatggcat gctttatcgt tctcatttca catcttgccg
     3841 ggatcaccga cgatgaggtt cgcaacagga ccgttgacga gctgagtaaa accgccgcgc
     3901 tacaggtttc gctcattcgg cagctcgtga gcaagcgaag ctcggctgcg gccgcgcggg
     3961 gaatgagcct gcgggaagca tacgaagagg cagccagggc ggggggcttc gagccgctgc
     4021 cggatgagcc atccgagcac tgaatacgcg acgtaggagg aaacgtggtt aagaagtagc
     4081 aggtcccgac tgttacaacg tcgatcgaga agatcatggc ctcgaccgag ttcgcattgg
     4141 gagcgatcga ccgtcgagcc gggcgtggtt atcgcagcga ttacacgacg tggagcggca
     4201 atcagcaatg gaattacgaa cgcggccgcg cctgggccac gacggcgccg cgttcggtgc
     4261 cactcaagcg caacggcaag ctcacgccag cggcgctgcg tttcgccggt gacatcattt
     4321 gatgtccccc caacagaaat cttgtttcgg gagactgccg taaggagcgt cagcatgagg
     4381 caggtggcaa aaagaagcgg aaaggtgcgc gcaaaagcgc gcaagcaaaa cgcgcgatcg
     4441 gcagtgaggg aaatcaccta tgctccgact gagaagctga ttccggtgct taaggccatg
     4501 ctcgacgacc cgctgcgcag tacgaagtac gacgaggaga cgctggaaaa ggcggagacg
     4561 atactgcgga tgttgcggga tctggagaag cgggggtttt tccgcccgtc gtgatggtct
     4621 ccccctaaat cttgcccgac tcacgccacg ctgcgtacta tcggcgctgt gctttgctac
     4681 aaggggcaca ctttcaggca gcaacgcagc ctcggcggga gcctccaccc cctccggggc
     4741 cgctggcgtt tccgggcgac cctcaaccga catctggaag gcgccgcgct gacgcgcaga
     4801 ggggtcgccc atgcgttttt cggtgttgag ggcgacgtgg gtagcctggc cgatatattt
     4861 cacatccatt gcacacgggt ggatttggca ttggtttttc aagagaattt tcgttaattg
     4921 ccgctctggc tgagccgcaa gcttgcgcgc cgcgacccag ctccgccctg gcggcaggtc
     4981 ttctttcttg cgttcgtcgg catacgcggc gttgtctcgc tggcggcggc gctggccatt
     5041 ccgttgacca ctctggccgg cgtgccattt ccctaccgcg atctcattct cttcgtgact
     5101 ttcggagtta tcgtcgtcac gctggtcggc cagggattgg tgttgccggg cgttgtgcgc
     5161 tgcgttgggt tggggagcca cgcggccgag gagcgtgagc gcgagcacgc agccgagctt
     5221 tccgcgcgcg ccgaagcctt gaacgtggtg caaaaccggc tcgaccgact tgcagccgag
     5281 ggccgaattg cacccgaggt tctggcaatt ctgcgggccc ggcaccacga tcgtgtggga
     5341 cggctgccga gaagtccgtc agatgggttc gaaactgctt ccgctgtcgc ggagcttaga
     5401 tcggacctga ttgccgccga gcgtgaattc atctatcagc tgttccgaga gggcaaaatt
     5461 accgacgagg cgcgccggcg catcgagcgc gagcttgatc tggaagaggc gggcatcgca
     5521 tgcaagaaag agggcggagt ggaacctcca ctctaggact cgccgcgaat aatctcagcg
     5581 atcctggacg gcaccgcgcc ggctggtctc accgtgacag gactcgcaca accgctggcg
     5641 tattcctgga ccgaacagga gcgctgtctc cgaccatgaa tggccgaata ccgatacatc
     5701 aagatcagac aaaccggtct cttgcgatgt caaaccaagt ctctggcatc agaacttgga
     5761 tattgaaaac tgccgatcag agactgacgc ccaaaattgg ccgattgccg ccgacaattc
     5821 agaaaattac gcctcagaga ctgcgtaagg tctcgctaac tcccggaaat ctggcgccta
     5881 atttggcaac ccggacagag catacaaaga ccggactggc tggctgggca cgtaagactc
     5941 gaaactcaga aatgtcgttg caaaatatcc ctttgaaagg ttgcacagat ttccggtgat
     6001 ccagccgcac cgtccattgg agaaaagttg gagatgcggt aaaccggaag agcctaaatt
     6061 gccttaactc gctgaaatca ttggcgctcc ctaggggact ttaccaagca agcgttttca
     6121 gcatgttacg cgaaagtggg acagtaactg gttctatgat gttccaaggc ttttccggcc
     6181 aactgtccca ctgattggca ttttgcgatc cgcacaagcg acatggacgt gtgcgtccgg
     6241 cgtgctggca aatcgggtga cgctcggcgc aactgaccag cgcgctcgat ctgttcccac
     6301 aacataccct ttggccaacg gtggtagcgg gggcgcgcta cagccgttgg aaaccggcca
     6361 tcgaagcggc tttcgtgttc gcggcttagc ggctgggaaa ataacccatc cagggtcggc
     6421 gcaacgggac tccgttttgg tcggtgggcg ggggctggga tggagcctcc gggccaggaa
     6481 aggtgttgca cgtagcctcg atcattccag agccctcggg agatttcccg aaatgaagtt
     6541 caagcacact cccccacatc ttctgctttg tgcgctcttt cacttacccg cgctccctcc
     6601 tggaaataag gcagcgctgt acggccttcc tatcgcctcg cccgccggga acggtccccc
     6661 atggttcaca aaaatgctca tgttttcttt gagagcggaa ccccgtccag cgccggaaca
     6721 tgatcgcgcg gcggaccccg tccagcgccg gaacatgatc gcgcggtttg tacatgccac
     6781 gtacggcctt cctatcgcct caccccggcg ctcttccaat gattaacggc cagtgaaaag
     6841 cgctcttcat cagattggcg ggtcgctggg gcctgcaacc cctcgacaat gtcagtgagg
     6901 aagttgattc gttgagctac gcttgatgag aggtgcgggt gtagcggaat agtgaggacg
     6961 cgcggttgtc cgaactctcc gtgatttcgc gctcaaatgt tcggatagcc tgttctatcc
     7021 gcagcttcat ttcgggcatg gaatgttttt cgatcgcgta gatgacgctg tcgttgagat
     7081 caaggccgta ggggaccgaa atcatgttca ccaaacttcg tcttaagcgg ggtaggaatg
     7141 ttatcgatgg cgcaattggc acgcatactc gatgccctat ttccattgcg acatcggaaa
     7201 aactgatgct taatcgtgtt gatcgttctt gagccgttcc ctccggtgat ggttcaggat
     7261 tgccgtggaa ttttcgccag tgaagtcaaa cactctttga gatcagggaa aggccatgcc
     7321 cgccttcatc tgcaacacct gcggcacgca atacgcaccg acggagaagc cgccggcgtc
     7381 gtgtccgatt tgcgaagacg agcggcagta cgtcacgact gccgggcaaa gttggaccac
     7441 gctcgacgtg cttgccaagc gcaacatgaa cgccattcgc gaactcgaac ccggtttgat
     7501 tgcgcttctg acgttccccc atttcggcat aggccagcgc gcgctgtttc tgcgcacgcc
     7561 gcacggtaac gtcctgtggg actgcatcgc gcttgttgat cgcgcgacga tcgagatgat
     7621 caacgctatg ggcgggatca aagccatcgc gatctcccac ccgcactatt acacgaccat
     7681 ggtggaatgg agcgaagcat tcggcggcgt gccgatccat ctacatgaag ccgaccgcaa
     7741 gtggatcatg cgccatagcc cctccattgc tctctggaag ggccagacta aggagctact
     7801 gccgggtctt accattatct gttcgggcgg tcattatccc ggcggcaccg tgctacactg
     7861 ggcggcgggc gccggaggga aaggtgcact gctttccggc gacatagtgc aggtcgttca
     7921 agacaataag tcagtgagct tcatgtggag ctttccgaac gtgatcccgt tgtcggcgcc
     7981 gcgcgtcgaa ggcgtggtct actcgctcaa agcttacaat ttcgaccgca ttcacggcgc
     8041 cttcattgac cgaaccgtct ggtcagacgg taaaggcgtg ctggagcgct cagccgcacg
     8101 ctatctgaaa atcattcgcg gtgacggcac atacgaattg cagtgattct caagcgttgc
     8161 ttcgccttcg caggcggacc aaattcagtc tttttgtcgc agacggtgca acgacagccc
     8221 cagcttcaga aaatgtggcc tagaccggcg gcaaaatgga ttcactatcg gcgctgcttt
     8281 ctgtacagtc agggacgtcg ggaaccgcag gctttcactg ccatagcgag tgatcgcaca
     8341 tgtgggaatt tgacagtgac ctcgccgcag tccgcttaca gcgcaggcaa atcttccgct
     8401 cagtaagccc gcgaggctcg attattggtt agccagccag tccggtctcc gcgcgctaac
     8461 atgtgaagac cgctcgaaac tcaccgttgc ttttactgcc gtcggtgctt ccaccaccga
     8521 tgcaagctcg gcaatgaggc catcgtagag taccacagcg tctttggcac gtccgtagac
     8581 gcaggcagcc cttgccgcat gatcgcgatg ctcgcctcgg ttaaattttg cggacgcggc
     8641 attggggact atgctgcgcc tgagccggag gtgccgacgt tcatgcgctg ccgaactcct
     8701 aggcagcaag ccaaccaagg ctcaccctca ggcctcggat cgagcagaat agtcagcatc
     8761 ggcacgcacg cgttagtctc gtacgcggct tgcgactgag agggacaacc atgattgtca
     8821 gatacgaatc tgatggctcg atcgtcatga tcacccagaa cgatcacgcc caactctcgg
     8881 ggctgtttgc tgcgcattgg ggcaatggga ggttcgagaa gccgcggcct tatgggtcgt
     8941 tggtgcgcgc cgccatgttt catgaccgtg gctggatccg atacgaaacc agccctcagc
     9001 ttaacccgga aaccgggaag acgccgaact accgtgaagt acccaacgat cggatgcaac
     9061 tcgaagcgtt cgaatgggcc ggcgactggc tgagcgccat cgatccctat gcaggactga
     9121 tgatcaccaa gcataggtcg ggcctttggc aagggcgcta cggcgtcatc acccatccgc
     9181 ccgcgattca gcgcggcgcg ctgccgcccg agatccaggc gttcatcgcc aaaagcgagg
     9241 ccaagcagaa agtcgcggcg ggtaagctcg acgccaagga aatggcgatc aactataacc
     9301 tgctgcaggt ctgggacatg atgtctctat acatttgcag cacagaagta ctcaagccgg
     9361 accggatcga gccggtgccg gtcgcttatt ccggcgacgc gggagtcaag atgacgctca
     9421 ccccgttgaa agcaaacacg attgtgctcg atccctatcc gttcgatcag tgttcactaa
     9481 cggcgaacgt catcttccgc cggttgcggc agaccagatt taaagactcg gccgagttgc
     9541 aaacggtcta tttcaagacc gcgccgcaga tcgcgtcatt cacactggta tcgcccgcgc
     9601 tgaactagcg gacccataga gcctgaacga aacatctttt ctatcggtat cgtcgtcgac
     9661 gtcattctgt gttcgtgggt gtagcggacc gcggtgtcaa tggtcttttg gtttgtctcg
     9721 ctaggaatcg gccgacggat cgggagcgaa cagcgctcgc tcatcttccc ggtctgcagc
     9781 gaaatcacgg cccgcggaac tgcacgcatg ttagccatcc gctgataaag caaggtgttt
     9841 cgcctcttcg aacgcaccgc aaagcatggc cgtgcaatcc gatggggcaa cacggctcgc
     9901 gcgaggcaat ccagcgcatt gcatcgctgg catgcccctt gcataatgcg aagcggattt
     9961 gtcgtgtcgc aaggctacgc cgcaaaatcc gatgaagccg atgcgcgccg ccgattcgct
    10021 cgatacgagc accaaccgcg cgccggcgct gatcctggcg cgcggcctgt cgaagacgta
    10081 catcacggct tccggcgagg tgacggcgct caagagcctg gacttcgaga tttacgacgg
    10141 cgagtttgtt tcggtcatcg gccagagcgg atgcggaaag tcgacgctgc tcaaggtgct
    10201 ggccgggttg ctgccctgca ccacgggatc tgttgagctg ggcggcaagc cgctgcgcgg
    10261 gccgacgcca gaggctgcgg tggtgttcca gtctccggtg ctgctgccgt ggcgcacact
    10321 agtcgacaac gtgctgcttc cgatcgaatt ccgcaagttg ccgacagcaa gctatcggca
    10381 gagcgcggcc gacctgctgg cgatggtggg cttgaccgac ttcgcgcaac gctacccgtt
    10441 cgagctatca ggcggcatgc agcaacgtgc ggcgctcgtt cgcgcgctgg tgcaggaccc
    10501 gcgccttctt ctgatggacg aaccgttcgg cgcgctcgat gcgatgacgc gcgagcagat
    10561 gaacctcgaa ctactgcgga tctggagtca gagccgcaag acggtgattt tcattaccca
    10621 ctccatcgcg gaagcgatct ttctctcgga ccgggtgatc gcgatgactg cgcggcccgg
    10681 caccatagcc gacgtcatca ccatcgatct gccgcggccg cgcgcgcttt cgagcattaa
    10741 caccgaacgc ttcggccgct acgctgcgaa gctgcgctcg ctgctggacg ctcagggagg
    10801 catgttctga tgatggtgca agccgtggac agcagatccg tggattccgg cgcacaggct
    10861 cgggagccgc gaccgggcgt gctgcggcgt tatccgcaat tcacgatttc gcctacaatc
    10921 cttgtggctt tgctggtggc ctggtggctt gcgaccgacg tgctgaacac gccgggttat
    10981 atagtgcccc cgccgcaaga tgtcttgcgc gccgccgtgg gcggcctaag ccgctcgccg
    11041 tgggacaagg ccggctactg gtatcacgcc gccattacag tctgggaagc ggtgctcggt
    11101 ttcgcgatcg gctcggcgct cggcgcgtgc ctcgggctcg tcctgtcgca ttggccgatc
    11161 ctcggaaaga gttggtatcc ctacatcgtc ggcttccagt cccttccaaa ggtcgcgctt
    11221 gcgccgctga tggtggtgtg gtttggcttc ggccttgagg gcaaggtctt catcactgcc
    11281 atcatcacgt tcttcccggt gctggtgaac atgatggcgg gctatcaggc agtcgagccc
    11341 gagcggatcg agctggcgcg ctcatgcaac gcgagcgaac tggacctgct tacgaaaatc
    11401 attattccaa gctgcctgcc gttcctgttc gccggtctcg gcgttgcctc agttctgtca
    11461 atccttggcg cggtagtcgg agaattcgtc ggggccagcg ccggtctggg catgctcttg
    11521 atgcagtaca atcaggcgat ggagatctcg ccgatgtttg ccgtcatttt gctgctggcc
    11581 gtgcttggtt ttctgatgaa ttacgccgtc ggtttagtcg agcgccgcta ttgcttctgg
    11641 gcgcagcggg cgaacgctat ggcgcgaact gactgggacc gaccttaagc gcggaggcca
    11701 tacatgctca ctcgactttg ccacgtcaca ctggcgctga cagtagcaac cggcgcatct
    11761 gggacacacg ccaccgacgt tgctgcgcaa tccctcaaga agatgacgtt ggtgcagatg
    11821 cacccgatca tgggcatcgg cgaggaggtg tttctctacg ccgtgcccaa acgcctcggc
    11881 tacttcgcgg cggaagggct caatgtagac atccaaaatt ctcagaccgg catgatctca
    11941 gcgcaagtgt tgcagtccaa caacgcccag gtcggcacca ccgccgccga ggcggtcatg
    12001 gcggtacgag agcaaagtgg cgatctgatc tccttcttca acctcaagcg caatgcgggg
    12061 accttcctcg ttgttcttaa ggactcaccc atccagaagc tcgaagacct caagggcaag
    12121 accgtcggcg cgccctcgtt tggggccggc ggcggattgg ctttaaagca gaacctgagc
    12181 gaaatcggca ttacgcctga tcagtacatc ccgattgcaa ccggcgccgg gccttcggcg
    12241 atcgcggctt tgcacactgg caagatcgat gcgctagtga tgtgggatgc aatgctcggc
    12301 gcagccgaga acacaggact cgcgctgcgc gtggtcaata ttccgcttga ggatcgcatg
    12361 gttgggacca cgctcgcgac gaagaagtca ttcgccgatg caaacgcaaa ggaacttgca
    12421 ggctactgcc gggcgatgac caagggcctc gtttttacga tgacgaatag ggcagccgct
    12481 atccgcattt tctgggatga gttccccacc acgaaacctg cgaacttgga cgatgccact
    12541 gcgctcaaga acagcgtgca tatcatggat cggttcctag agatggcgct tcaaggccaa
    12601 cccgagggct cgcgtcttgg cgaattcatt gcgacgaatt ggcagaacac gcacgccgcc
    12661 tatgtcaagc tgggcacgct gaaaggaacc gaagccgcga ccgactctta cacggagcat
    12721 tttttggcag cctgcaatga cttcgaacgt gcgactgtcg tcgcgcaggc aaagagcatg
    12781 aatcaataga tcgcaaacgg accttgcgag agtcagggaa aggccaagga atggcgtaaa
    12841 acccagacat ggcgcacatg agcagcaatc gagccgcaaa aaacaggccg gccaacacgc
    12901 cgaaatgaga agcctagctc acttggactt caacgaactg aattgttcca ggacttcgat
    12961 cggttttgaa aacatggccg agaaggctct cggcttcaga cacgacgccg acgccttcgc
    13021 tcagggcaag aacactcaca acgatattgg ttcgagacga tctgaaagtt cggattgggt
    13081 gccgtcgtca tggcaccccg ccgcctcatg gcctcctcta acttggccgc ggcgtcgtcg
    13141 cctatcaggt gcgtcctaag catatccaaa accctcggat ttgttgtcac tatgcgatgc
    13201 tgttaggttg ccgtgttcga gccgtcagtg cattagccgc tgcggtatcg attctggcaa
    13261 tttagcacga cctcggctga tgattgagac aaacaagagc taaatgagga agccgctgac
    13321 gacatctgtc gcgcgggagg cacctcactc tcgaacctac ttcgcgtcaa ccacttcctc
    13381 agcgatctca ccgtaattca tcgtgcgttg cgcgcctggc aagagaagct cgcgggcgca
    13441 tctatcccat tcgacgcggt ccgtacgccg tcgccgatgc tcgtgcctgg ctgcaatatc
    13501 gtggcggaca tgtgggtcta tcatccatag cgcgaagacg atcttggagg acggtttatg
    13561 gaacgtcggc gagttgcaaa ctgccaagag tgaaaatcat attttggtcc agccacccaa
    13621 ccataaaaac attggagact gtttctgcat cctgggtcat gctagccgac cgggttgatc
    13681 aactttgata tgggcattac ggtgatggtt ccgtttatcc ggaggcagcg taccgttctc
    13741 gcgaccgggc cggatgatga atgtctcgaa caacgggggt ggagatgact ttgcggacct
    13801 ggtttcgaat gctgggcatc actgtcctta ccttcagtct tcccgaagta gcacttgctg
    13861 catcaagcct cgacaaggtg aagcaatccg gagtgattaa ggcctgcctg gcccagcaac
    13921 aacccgataa ttacaaagat cctcggtcag gccagtggac tggtgtcatg gtcgacctgc
    13981 ttaacgaact gtccaaatgg atgaaggtga aaatagatat tgctgaggtg gggtgggatg
    14041 tcgcagttct ttcgctcaag caagggacct gcgatctatt cgcttcctcg atcgtttata
    14101 cggcgccccg tgccatggaa attgctttcg tgacgccttt cggagcgaaa ggcgacaaca
    14161 tcgtgatcga taagaagaac ccgaagaaca tcaaagtaca tgcggatcta aacagcccaa
    14221 atattacaat cattgccgag cttggcacgc gggagcatga caatgcccaa cgtttctttc
    14281 ccaaagcaca tatattggcg gtcaaggttc caagcaccgt ccaagtgata gactgggtca
    14341 agcgaggcga cgctgacgcc gctgtactcc cgacgatcac ggctcgatgg tggttgaacg
    14401 tgcctgagaa tgccggatgg gctgcgatgg gatttcccga caacgatttt ggcaatgcgc
    14461 cgaatggatg ggccgtgcgg caaggcgatc cggactggtt ggatttcctc aactcgttct
    14521 ctggttgggt aacggcaaat ggcatggcca agcagcttta cgacgagtat ttagagcgga
    14581 cgaatccatt ctcaaagaag gactgaattc cccgagcttg acagtcacga cgtacagcag
    14641 acgcggagcc ctgttgcatg tcgttcggcc gattttaccg actgatcgct gataatgccg
    14701 atgtgatcgg cttcggaatt tgggtgacga ttcagcttgc catcgtcgcc atgctgattg
    14761 ctgtcatcct cgggttgatt gcgtgcctcg gatcgctgtc gcgtgctagg attctgcgct
    14821 ttccatcaat gctatttgtc gagttcggcc gcaatacgcc tattctggtg caactcatct
    14881 gggtgcactt tgcctggccg gagattttag ggattaagtt tagtgcttgg actagcgcgg
    14941 tcattgccct ctcgctgcag tcgagtggtt atcttgcaga ggagtttcga gcgggaatcg
    15001 agtcaatcga caaaggtcag atcgaggcat cacaagccct cgggatgact tatcagcgct
    15061 taatgcgtcg catcgtagtc ccgcaggcct tgatgaggat gattccgggt atcctcaacc
    15121 agttcgtcac gtgcttcaag tcgaccagca tcgtatcggt catcgcggtc ccggacttga
    15181 tgtaccaagc tggtctcatt gtttcgatga cgtttcttcc catgccggtc tacagcttcg
    15241 tcgccttcct atattttcta atggtgatga tgattgcact aggcgtgcgt tttgccacgc
    15301 gtcgtctccc tcagttcggc tacttcaacc aacgtcacgg cggggagtga tgcagctcgg
    15361 tcatccggta aagccgctcg tacgtctctc tgccgtttgg aaacgattcg gcaagctcga
    15421 cgtgttgcga ggtatcgatc tcgatgtgaa tgatggtgcc actattgcca ttatcgggcc
    15481 atcgggatct ggtaagagta ccctcgtgcg gtgcttgaat cacctcgagc ctattcaaga
    15541 tggagcgatt gaaattgacg gcttcctgat cacgccgcgc ggcgtcgaga agagcggccg
    15601 gcttctggga cagcgcgagg tggccaagtt ccgcactttg ctggggatgg tttttcagca
    15661 attcaatcta tttccgcacc taaccgttct ggggaacata cttgaggcgc ccactggagt
    15721 tcttggtcgt gatcgggcga cggtcactga agaggctatg gaccttctcg cgaaggtaaa
    15781 tctggctgac aaggcgaatg tgtatcctgc ccggctttcg ggcggtcaac agcagcgcgc
    15841 tgccatcgtt cgctcactga tcatgcatcc caagatcatg ctcttcgacg agccaacctc
    15901 cgcactcgat ccggaattga taggcgaggt gctcaaggtg attggggaat tagctatcaa
    15961 aggtcgcacc tccataatcg tgacgcacga gctcgcattt gcccgagaca tcgcgagcaa
    16021 gatcgcgttt atggatgcgg ggctgatcgt cgaggaaggg cctcctgaca aattgttgac
    16081 ctcgcccaaa ctggatcgga cccgagcgtt tattgctcgt attctgtagt tggctaatgc
    16141 agaatcctaa acattcggat aaacgcgcgt ttcatgaaga aatcacacga cattctgatc
    16201 aatctcaacc gcctcaatca gaagatggat gagtacggtc ttgcaggaat cgtggctcgg
    16261 gcaggcgtca actacacgta cctttcaggc gtttcatacc gcggaacatt agcgcggcac
    16321 ctagacttga cggattcccc acggggtacg tttctggtct ggccgcggca tggcgaacca
    16381 agaatcgtgc taaacgcctt cgccgaaggc ctaactcgtc gcgattcttg ggtagagatc
    16441 atcgacgtgt atgaaggcta tagcgagcta cccgtcgagc ggctcgcaaa agtcatatct
    16501 gatatgggtc tggcagacga gaccgtcgga ttcgaacaga acttcgtcag tgcagcggac
    16561 tggggcatgt tacgatcacg ccttcccaga atgcgcatgg tcgacagcac gacggcaatg
    16621 gatgaggtgc gcgctattaa aacggccgga gaggtttccc tgcttaaacg tgcggccgat
    16681 atactggatg acgcctacac tgctgtgctt ccaactgttc gccctggcat gcgcgagcgc
    16741 gacgttcacg cgcggcttgt agctgagtgc ctcgttcagg gatgcgaatt cgttcacggc
    16801 atccttaatt ctcagcgcaa taccattccg tatgccgggg aaagcgattt cgcctttgcc
    16861 gatcgggacg ccattcgaag tgactatgtc gcgtatttga tgggttatcc ggggcatcaa
    16921 tcccggtgcg ccgtcgttgg caagccaact gcggaacagc gacagcaata tcgcgtggtg
    16981 cgggacatct atcgcgcatc agcggaccag tgtcgagttg gaaagacggc cgatgagata
    17041 taccgtaacg tagtcgacag attctcagca gccgggatgc aatatactac gctactcgct
    17101 ggccacagtg tgggctgctg gtggcatcag caggagcctg tcattgcccg cggcaacctg
    17161 cgtcggcttg aagacgggat ggtcattgcc atggagccgc acgtcaatca ttggcacatc
    17221 caggatatgt ttattgtccg cgaggagggc cccgaactcc tttccgataa attcaacacg
    17281 gacgagcttt tctcctgcgg gtgagtcagc cgcacaagca gacggccgcg tgagcaaccg
    17341 cctttgccgc tgtaacgagg tctgctagga gcacgcgttc gtcgggggtt ggccattcct
    17401 tgtagatacg aatgtctcca gggccgtact gaaccgtcgg gataccaagc ttctcttgga
    17461 tgatattgcc atcgccaaca ttcccgagcc ttccatcggc gccgatgtag ctttgctcac
    17521 ctgaggcgat ggtctgccct tcggctaagg cgcgaaccag agcgtgatct gatgggcatt
    17581 ccataggagc ctgattccat cccgactcgg tgcctcgcgc aggaatttcc agcgtgcaat
    17641 tgaacgctgg atgctcgcgc tttatcgcag ccagaagtcg agtgagatcc tttcttatcg
    17701 aggccgtcgt ctgacccgga acggtgcgga actgcaaggt caattcgcat tcgcggcttg
    17761 atcggcctgt gtagtttttg aagtagtagt gctctttgtg gatcacatcg aatgtatgcg
    17821 tcggaaatcc aggaagctca ggatgcgatt tgaaggtcat ccatccacca tggggaatgg
    17881 gctttagact gggcccaatt cgacggacga tctcggattg ttgttcgatc ggattcatgt
    17941 atgcagcgcg ggcctcggcg ctatagcgga agaacagttc tggagccgtg cagacgaggc
    18001 gcgccattac aacaccgacg cagacattgg cgatcgtgtt gttggaatgc tccatattga
    18061 tgcaaaggtc ggccttgacg ccacgatcga gcagcgctct ggtcccagca ccaccgagct
    18121 tatgagctat tacgggtgtg acgattacgt cacctctcaa tcgggtgccg gagcggacga
    18181 ttgcctcgac cgcgctgata gcggcaacgc agccgccctt gtcatcatgg gcgccaattc
    18241 cccagatcca tccgtcttcg aatttagcgc ctaatgggtc caccgtccaa ccagtcatct
    18301 cggcacccgg atccatgtgc ccattgagca tcagcgtcgg accttcccct gtgccgcgca
    18361 gccgcccaat gggctgccgg ctcttcttcg ccggatcttg tggatggata acctccatca
    18421 tttcgacatc cagacctata tccgccatcg tactggcata caggtcggca atttccccct
    18481 cctcgaaagt cgagctcggg attctgatcg agcgctgctc gagctcgaaa attcgacgct
    18541 cgttcacagt cgaaatagcc ggtccggaat caaggcgctt ttgcatttgg gacccctcaa
    18601 agggatgaac gggctgaggc tggatattga aatcaggaga cgcgcccttt cccattcggg
    18661 gatgagctca cgctatcgga aacggccatg attaccagat ttgctcgagc ctttggggca
    18721 tccggaccaa gatgccgaga aaatctgatt cgcagaatga ggggttatga aacggggcag
    18781 gagccgtgac gggtcccacg tgatcggaag acccgtggcc cggatcgcgc ctctggcgca
    18841 atccgggtcc cggatgaaga cgcatccgcg ctacggctcg aagttcggcg aggcggccca
    18901 ttcctggaag agcatctcgg cttccgccac gcagatacgc tctgaaggca atttccgcga
    18961 cgcaggcggg cgccttacct catcgaccaa tgctttggtc gaaaatccga tgcgttcaca
    19021 gtcctcctgg gtatgagcgt aatacatctt gctgattttt gcccaccaga tcgctgaaac
    19081 acagagaaga cacggctcac aggtggtgta gagctcgcaa ccgctcagat cggtcgtgcc
    19141 gagccgcctg cacgcatcgc gaatggcaac aatctcgccg tgtgccgtcg ggtcaagcag
    19201 ggttttctgc tgactgcggc cctcgccgac gatcttgcct tgcctgacaa cgacggccgc
    19261 aaatggacgg gcatctttcg tgcgcaaacc tgcacggccg atttcgattg cgtgcgccat
    19321 gaatttctcg ggagtcatta acatcctctg acaacaaaac tcggcccgga taaagccgag
    19381 tgcgaatagt acaggacgtt ccgctcgaat cgaggccctc atcctgagat gcgagcgtcc
    19441 ccgaccctcg aaggatgagg gggggccgaa tgtccttgcg tctatggcgg tgacttgtgg
    19501 aacggtgtcc ccccggtgtc gttggcggcg aaccaccgtc atgcccggca acgcagtcaa
    19561 gcttgcgcag actgcgtaaa cttgtctgcg ctcccgggca tccacgcctt tgccgcggcc
    19621 gcaagacgtg gatggccggg acaagcccgg acaggacgac tttaacctca ccggaaaatg
    19681 ctttaatcat cgaaatgcgc cggctgagac ggccttggcg gcaggcccgg cgctctgcaa
    19741 ccaggcggag aacgggttca tgcctagcct cgatatttgc agcgccacgc ggccggccgt
    19801 cgcgctggcg gcgggaatcg gattgctgac tctcgccttc ggggcacccg cccaggaaaa
    19861 tggaatcacc ccctctgaaa tctcgatcgg ggcgatcggc gccctgaccg ggccgctcgc
    19921 cttcatcggc acgcccggcc gcgacagcat gatgctggcc ttcgacgaaa tcaatgcaaa
    19981 gggcggcgtc tgcggtcgca agctcaggct cgatttcgag cacgcctcct cgccggcgga
    20041 gagcgtcgcc gcagtcaaga agctggtcga gcaggacaag gtgttcattc tggtgctggc
    20101 gtccgggagc accggcgctg ccgcggcggc ggattacgtg cgccaggtcg gcgtccccac
    20161 ctacaatctc tacggctcga caccaatcat ccgcgagccg ttcgccaaga acgtcttcca
    20221 cggcgccgtt gtgccggtgc aggtatcggc cgccgctctc atcagcatgt tctacgacgg
    20281 cgaatacacg ccgaagaagt tcggcatcct cgccggcacc tatgccttcc cgcaggccac
    20341 cttgaaggcg gcgcaggaca ttctgggcca aaaaggcgca acgttcaccg tggagcagtt
    20401 cgacatcggc gcccgtgatt tcacctccca gctggtgtcg ttgcagcgcc agagcgtcga
    20461 ttccgtgctc gtcctgggaa gcttctcgga ggccgggttc gccatcaagc aggcgaggga
    20521 aatgggtatg gccaacgtgc gctgggtagt tgacggctcc gccgtctcca ccgccatcat
    20581 cccgatcctc ggcaatgccg atggtattcg cggctattac aatgcgccgg cctttccggg
    20641 gcagacgccg gccacccgcg aattcgaggc gcggctgaaa aaacagctcg gctcgatgcc
    20701 acaggggcgc cccagcgtct acgatctgat cggctatggc tcgggctacg ttgtggcgga
    20761 ggcgattggg gcaacgaatt gtcagttgac gcgcgacaac ctgatcaatg cctggtcgaa
    20821 tctcaaagat gccggtccga cccgccttga cgggctcgat atcacgtttc ccgaaagcta
    20881 ttcgccaacc gatcaccagg gcgtctatcg catcggcgcc gcgatcgtga aggacgggca
    20941 gtggcaggtc tatcgcgtga tcgataagcc ctgatcgcgc aatgctcacc gagctgggat
    21001 tcgtcgttct ggcgggcatc aagttcggcg cgctctatgc gctggcggcg ctcggcctcg
    21061 tcgtggtcca caaggcgacg aagaccgtca atttcgccca cggcgcgttc gcgatgctgg
    21121 gtgcgttcgc cagcttcctc atcgtggtga actggcactg gccgtattgg gcggcgtatc
    21181 tgctgattcc gtgggccatc gggctgatcg ctgccgcgat ggaatccgcc attttgcgcc
    21241 cgctgcgacg ggccgacatg ttcaccgtcg tgatcgcgac ggtgtttttg ggcatcgcca
    21301 tctcggaagg atttcgcttg acctacaaca ccgagctgtt ggcagtgccc tccctgtttg
    21361 cgggacctcc cttcatcgtc gccggcgtcc tcatcaccag ggatactcta tgggtcatcg
    21421 cgggggcctt ggctgcggcc ttggcctgca tcgctctctt cagctacggc aggtttgggc
    21481 gcggcatgcg tgccatggcc gcgaatatcc gtggcgcgca actatgcggc tactcgatcg
    21541 accgcgtgta catgctagcc tggttcctag gcggcggact cgccgggctc accggcgtgc
    21601 taatcgcgcc ggttacgggg gtgtcgattg acctttcgat tgcaatcatc accgccggct
    21661 ttgtcgccgg tgtgatcggc ggcttcgaca gccccaaggg cgcgatcgtc ggaggcctgc
    21721 tcctcggcat cgccgagagc gtgtcggcgg catacgtttc gagcgcgatg aagaattgcg
    21781 tgagcttcct gctacttttc ctggtgctca tgtggcgtcc gcagggaatg tttcccgaaa
    21841 ggtccgggcg cagtgtctga ccgccggccg gaccttgcgg ccctcggacc ggccctgctc
    21901 attattctct gcgcagccgt ggggcttcgg ctcgacagct atccccaata tgtcattgcg
    21961 gtggccatca tatcgatgct ggttggcact gcgctggtgg tgctggtcgg gctcgcgcgc
    22021 tgcatcacgc ttgcctccgg gtcgatcatg gccatcggcg cctatgtatc gacgctcgcc
    22081 gttgcgcagc tgcgcgttcc ttatctcggc acattggcgc tcgcgatcct tgccggcggc
    22141 gcaggcggct gggtgatcgc ctttctcgga acgcggtttc gcggccacaa tctggcaatg
    22201 gtgacgctgg tgttccaaag cgtctccatc atcctcattc gcgagagcag gtggctcacc
    22261 ggcggcgccg agggaattca cgttccggct ccggagattt ttggcctggc tatcgcgacc
    22321 gaccgcgcat tccttatcat gatcggcatc gcggtcgctg tcatgactct gatcgtcacc
    22381 atcctcgtgc gcggaccgtt cggcaagaac ctccaggccg cttccggcaa tgagattgcg
    22441 gcagaggcat tcggggtcag cgttcgcggc tacctcaccg cagcgttcgt cgtgagctcg
    22501 attgcgatcg cgtttgccgg tgcgctgctt gcaccacgtg tgcgcattct cgatcccgac
    22561 agcttcggcg tgctggagtc gatcttcatg ctcgcctacc caatcgtcgg cggcatgcac
    22621 gcggtgtggg gcggcctgat cggcggcggc ggcttacgca tcctgccgga actgctacgg
    22681 ccggttgccg attatcagga actgttcttc gctaccctcg tgatcttcgt ggtgatgttc
    22741 ttcccgggtg gcttgaccga gctcatccgt cgtgtcgttg catcgaaggt caaaccgact
    22801 ccgcccgcct tggcgccggt gccggccgtg ccggctgctc cgacggaccg cgacgaaacc
    22861 ggcccaggcg gccacgaatt gctgctggtg gaatccgtcg ataagcattt cgatgctttg
    22921 cacgcggtta acgacgccag cctgcgcgtc cccgcgcgcg ccattcacgg gctgatcggt
    22981 cccaacggcg cgggcaagac atcgctgttc aacatccttt cggggtttct tgccgccgat
    23041 gccgggcggg tcacatttgc cggcacttcg ctgctggagc tgcgcccccg cgatcgcatc
    23101 cgcctcggca tcacccggac gtttcagaac gtcgccgtat tcggacagct ctcgtgtctc
    23161 gacaacgtga tcattgggct gggccgtaac gccgtccttc atgccatggg cgcgagcttc
    23221 gatcattttg ctcgcggcgc ggccacgcgc ggcgcgcagc gcgcggcact tgccgcgctc
    23281 gagtcggtgg gccttgctgc ccaggcttac tcccgcgccg gatcgctctc cctcggcgat
    23341 cagcgccggc tggaactcgc gcgcgccatc gtctaccacc cgcggctgat cctgctcgac
    23401 gagccggtgt cagggcttgc cgagtccgag accgaggacc tgcgcgaact cctgctgcgc
    23461 atcaatgcgg aacgcgggat cgcgatgctg gtcgtcgagc acaacatccc gttcgtctcc
    23521 cggctgtgcc ggagcctgtc ggtgatgggt ggcggccgta tcgttgccga aggcaagcca
    23581 gcagacgtga tctcgctgcc cgtcgtgcag cagctctatt tcggggagca agcgacctca
    23641 tgatcgcatt gcgtgccacc gacctcacag tagcctatgg acctgtcgtg gcggtgcgcg
    23701 atctgagctt ctcgctcgag agcggagagg cattggtcct gctgggcgcc aacggcgccg
    23761 gcaagacttc cgctgtggaa gcgatcgccg ggctcctccc caagcgccgc ggcaacgtgg
    23821 agtttctcgg ccgggacatt tcccgcctga gcgccagcgc catcgtgcgc agcgggttgg
    23881 ccctggtgcc gcaatggcgc gagctgtttc ccgccttcac cgtggaagag acgctgattg
    23941 cggcaaccac cgccgccatt ggacgcgcgc cgacgccgct taactcggtg tacgagctgt
    24001 ttccggttct gcaggagcgc cgccggcaga cggcagggtc gctctcgggg ggcgagcagc
    24061 agatgctggc catcgcccgc gcactggtga ccaatccaaa gctcctgctg ctggacgagc
    24121 cgtcggcggg actctccttg ggcatcgcgc gcacgctgat cggggccatc aaccgcatcc
    24181 gcgaaggcgg cgtggccatt ctccttgtcg agcagaacat ggaaattgcg cgggcgatcg
    24241 gcgagcgatg ctgcatgctc cgtgccggcc ggctcgcctg ggcgggctcc atgcaggaag
    24301 ccatggcaag aaacgaagcc gaacgcgtgt acttcggcgc gcatgggcgc gtttgttgag
    24361 gagcatcgca tgacggtgat gataatgatt cccgcaatga cgtggggatg attttgtgac
    24421 acagtgcagg taaagcacgt tccggtattt ttgatcagca gcgtcacatg ctcgacctgc
    24481 tccctctccc cgtaagcggg ggtggcgcaa cgggaagagg gagttcggcc gcgtatgcgg
    24541 cgccgttgcc tgcgtgcaaa gaacgcaaag cgtgacgaat gaccgcattg ttcagcgttg
    24601 gtttccctac gggcatggaa gggttgacct atccgatccc attttccaat cccgaggatc
    24661 tggttcgcat cgcccaacac gcggaagcgc tgggctacca ttcggtgtgg ggcaacgatc
    24721 atatgaccac gcagcactac gtgcgggcgg agtttccggt gccgccgcgc ttctgggagc
    24781 cgctggtcac ctacgcgtac ctcgccgcgc atacaaagcg gctgcgcttc ggtaccggcg
    24841 tgctcgtgct gccgatgcgc cgcgacatcg tggtgacggc caagcagatc gccacgctcg
    24901 atcatttcag caatggccgg gtggagatcg gcgtcggcat cggcgcctac cgcgaggagt
    24961 tcgaggcgct taatccgcag gccagggtgc atcgcgggga catggtcgtg gaaggcgtca
    25021 cggcgttgcg actgctgttc tcgcagcgcg cggcgtcctt cgacggcaaa tactacaaat
    25081 tcagggacgt cgagctgttt cccaaaccga agcagcaacg cctgccgatc tatttcggcg
    25141 gcaacaaccc cgcgcatctg cggcgcacgg ccgcctcggg cgacggctgg attcccgccg
    25201 ccatgccgca gcctaccttg cggcgcatgg tggcggagct cagggagatg acggaggcgg
    25261 cggggcgcaa attttccgag ctgcagatcg cgccgcagta catcgtgcat ctcggccgca
    25321 atcaggagca ggccgtggcg cgcttccgcc agagtcagat gttcaagcac ctcgagtcgc
    25381 tgaagagatc gacgctgcgc ggtcaggctt cggtcaaccc ggaggacgtc aatctggtcg
    25441 gcagcatcga catcgtgatt gcacgcgcca cggcgttgcg cgaagctggc gtgacgcatt
    25501 tcctcggctt gttcttcgcc gccaacagcg tccccgaact actcgatcag atgcagatgt
    25561 tcgcggaaga agtcaggccc aaaatcgcgt gaccgccaac atgaagtgtg ttgaatccac
    25621 gtcccgacgc catcccgacc aagcgaggcc gcctgatgag gcctcgcgcg agccgggatc
    25681 cagtaatcgg agcacccaaa aagtgctgcg cattttttgg ggaccccgtc atggccggcg
    25741 tgaaagcaga gacggcgtcg attactggat tcctgctctc gctcgccggg cacagcccgg
    25801 ctcgctcggc cggaatgacc agtacgtgtg ttgcggtatg acgtgcggac gcgtgagcca
    25861 ggagtcgcag taatggcagc cgatgcatcg gcggcgatcg ggcagcgttc cctgggcgac
    25921 ccgagcctgc tgttcccgct aaagccgccg ctcttgcgcg gctgcccgcg caccagcatg
    25981 gcggagatgc aatatccgct cgagatcgac ttcgattacg caaaggtctc cagggacatc
    26041 ttccatcaac cgccgctatc gggcctgcag cgctgggcgc cattgcttcc gccgctcgtg
    26101 ccggagctgt cgttgggcga gggcggtact gcgctcatcc ccagtcatcg catcgcgcgc
    26161 tgggctggcc tcgacggccc gatctggctc aaggacgaaa gccgcaatcc cacctggagc
    26221 cacaaggacc gattgaacta ctgcatcgtg tccacggccg tggcaaacgg cgcacgcggc
    26281 atcgccattg catcgaccgg gaaccacggc gcgtccgcgg cggcctacgc gcggcgcgcc
    26341 ggcttgcgct gcgtcgtcat ctccacgccc gacgcgcagc gcgccttcga gcattttcag
    26401 accgcgctcg gcagcgactt cctgttggtg tcgcgggagg agcgctggcc cacgctcaac
    26461 cgcgtcgtgg acgagaccgg cttcatgccc gcgagcaatc tcacccgctt ccacaccggc
    26521 aatccgttcg ggccggaagg ctacaagacc attgcctatg agattttcag tcagctgggc
    26581 ggtcgcgtgc ccggcaccgt cgtcctgccg acgggctatg gcgagctctt gttcggcgtg
    26641 gcgaaaggat ttcgcgagct caaaagtttc ggcctctcgg atacggtccc gcaaatgctc
    26701 tcggcggagc cggccgtgcg cgcgccgctc gctcgcgcca tgtcgcgcaa cgaggcggcg
    26761 gccgaggtca gcggcccgcc gtcgcgcgcc gccggcatcg cctgtaccgt gagctcctat
    26821 cgcgccgtgg tggcgctgcg cggcagccac ggccgtccgc tcacgtttca tgaggacgtg
    26881 gttgccgagg ccgcgagcga gcttgcgcac gaggggctgt ggcaggaata ttcgggggtt
    26941 gcggggttgg cggcgttgca cgaggcgcgg gcgcgcggcg aagtctttgc cgagccggtc
    27001 gtgaccgttc tgacctcgac tggattgaag gagatgccgg caccctccgt cgtatcatac
    27061 gccacggctc tgccatccct cgacagcgtc atggtctcgc tgctgcgagc gtaagagcgc
    27121 atcccatgca aatcggggtg tctgtggacg ggaacgagcc tccgtcttta ttgcgtgcgc
    27181 tgacggaagc agcggaggca gcgggcgcag ccaatctttg gcttgctacg cacctcttca
    27241 accgcgagcc gattgcctgc gccacggcgg tgctggcgca aacccgcagg ctcggcgtcg
    27301 tcctgatggc gatgagcccc tacacggtgc atccggttta cgccgccatg gcggcagcca
    27361 cgctcgacga gctttttccc ggccgcgtgc aactctgctt cggagtgggt gcacccggtg
    27421 ctctcgaagc agtaggcata accgcgccgc atccgatgga gacattgcgc gaagcgatgg
    27481 cagtggcccg cctcctgctc tccggcgagc cggtgacgtt ttcgggccgg cgctatcgcg
    27541 tgtccggtcg gcttgccatg ggcgcgcatc ggctgcccgt ttggctcgcc gcttccgggc
    27601 cgcggatgct cgagcttgcg ggcgaaacag ccgacggcgt ggtgatcagt gccggcactg
    27661 cgccggcatt catcggctgg tgcctgcaga gcgtacggcg cggcgaggaa cgcggcacac
    27721 gcgccgtgcg caaggcagca ctcgtggcct gctcggtgga tgcggatgcg caccgtgcgc
    27781 acgagcgcgt gcggcggcgc cttgcctaca tcttgcgcgg cagccatcac gcccgcaacc
    27841 tggagcttgc tggaacccgt ctggaccaga gcgctctggc cgaggccttt gcgcgggaga
    27901 gctggcagcg ggttgacgaa ttgaccggcg acgacgtggt ggtgcgtcat tgcgccagcg
    27961 gcacgccgga gcaggcgatg acgatgttgc aggcctatcg cgatgcaggg cttgacgaga
    28021 tcgtggtcta cggcatgcag gaacgccagc aacttgagga cgtgctcgca ttgatgtgcc
    28081 cgcaacgcgc atccgtcggg agatgaaaag catgcttgtc agggacgact tgagagcgat
    28141 tgccgaaaag gtgcgcagca aggtattcac tgcgtgcgat caggacgccg acgtcatcat
    28201 ttccaccgac ccgacgcatg tcggttacct gtgcggctat cgcagcatac tgttcgatct
    28261 catgcgcgat tatcgcgccg cggcgatcgt tacccgcgac aaggcggtgc tcgtgaccgg
    28321 agcaagcgac gtcgcggcgg cgcttgaagt gctttgcgat ccggcctgca tctatcgcta
    28381 tggcctgttc ttcttcgaat cgagcaatgg ggccggagtc gatctttctg cattgccgaa
    28441 gccggagagc acctttgtcg atgcgctgtg cgctgccgtc gccgcgaccg tgaagccacg
    28501 gcaacgcgtc ggcctcgatt gcgccagtgc ctgggaattg caggagctga aaaatctggc
    28561 cggaccgcgc agctttgatg cgcgcccggc catcctcaag gcgcgccgta ccaagcttcc
    28621 cgaggagatc gaaaagatcg cgcatgctgc gaccatcacc gagcgcggcc ttgagaaggc
    28681 gctcgcccgg gggaaagccg gcgtgagcga gcttgatctg agcacgatca tcgccggcga
    28741 gatgcgcgct ggcggcggcg tgccgcgctt cgtcgtggtg acctccggcg agcgcagcgc
    28801 gttggccgac gcctatgcca cgccggccaa attgcgaagc ggcgatctcg tgcgctttga
    28861 tatcggctgc acggtcgacg gctactgggc ggatatggcg cgcaccgccg ttgtcggcga
    28921 gcccacgcgc gagcagcagc agcgctacga cgctctgctc gcgggggagg aggcgcagcg
    28981 tgcgctggcg cgctcggggg tgagcgccgg caatctgttc aaggtggcgg tcgagcgggt
    29041 gcgccagggc gcgctgcccg cttacaggcg cagcaattgc ggccacggca tcggcatcga
    29101 tgtgcacgag tttccggcgc tcagtgcggc aaacgaaggc gtcgcgctgg aggacggcat
    29161 gaccttgtgt gtggagacgc cttattatca gatcagctgg ggcgggatga tggtcgagga
    29221 catgatcgtc gtcggtccga ccggcaacga gtgcctgacg catctgcccc gcgagctccg
    29281 ccggctgtaa tggagcacga ggcaacgctg taggctgcgc aaaatcgccc tgcgccgctc
    29341 gctcacggga gcagctggcc cgccgtggcc gacccgaagc cgaacccgct ctccgaagtt
    29401 ccggtcagca gctagccgcc aggttcgcat aatgagagtt atggaacggg aagggcaatc
    29461 ctatgccacg cctcaagccc gcgcagcgat cgcggagcgc cgtgttgctc gatcggcgtc
    29521 gtctggctag caccacgaaa tcggtcgata tttccaggcg gcgtctcaac gggtggtaca
    29581 agatccgcaa ggcggatgct cgtatggctg caccctgagt tttcgcaaat ggttccaagc
    29641 gaaacctgac cccaagctct gcttgggatc acgagcagat atcccattca cctagcaatg
    29701 tccgcttcgg gggcaaaacg gacgtgcata cgtctgtagt cgtgaatgac ccataagcgg
    29761 tcatagaggg ggccgcggtc gaacccgatt tgaccaagta atatcacttg tggtgtggcg
    29821 gccactagga tcggaatttt tctgccagca ctgcatatga cgccgactgt gtcttgcaac
    29881 tgtcattttc caatgcattc tcgtcgatgg tgaggcaggg gggctgccat tacaaagctc
    29941 ggcatttttg ctgcaacgat cctcgccgcc gccggcgtct tgtgcgcggt ggaggcgcgg
    30001 gccgccgacg ccgtcccgac caacgcttcg ccggccacgg cggcttccgc tcccaagacg
    30061 tgcgccggcg cttgggactt tattgacacg aactgccagc tcacctggta cggcatcacg
    30121 gtctacggca cgattgacgc gggcgtcggc tggcagagcc acggcgcgcc gtttgaccca
    30181 cggtccgctg tctcggcctc atacttaatc cagaaacaaa acaggtcggc gatgtggggg
    30241 gctgcaccca acgcgttgag ccaatcgatc attggcatca aaggaactga gccgatcggc
    30301 ggcaacacct ctttggtctt tgctctggac gctggctttg acccgtcgcg aagcccccgt
    30361 ccttatcttt ctaccagaac cggcacagaa ttccaatcgc gcacggcatt gcgagccgtt
    30421 gcgcggggcg acaagccagc tgacctaccg gtgcagcagc ctacttcgcc tcttcaatct
    30481 tagggaaggc cccggagttc ggctaccgaa cgggacccgc aatcgagcgg gagccctcct
    30541 gcacaagagc tagagaccaa ctttgacgag ttttccgcgt gctccgacgc agaagccgtc
    30601 tctggtggcg tagttcgcgt atccgtgctc gtggcaccac agggcgtctt gcgtattgtt
    30661 cacatgacga acga
//
LOCUS       DATLJT010000092         4856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_233401,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000092 DATLJT010000000
VERSION     DATLJT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233401"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VM910_02030"
     CDS             <1..819
                     /locus_tag="VM910_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61364.1"
                     /translation="RLMAEWLQQRLGQTFIVENRPGAAANVATDMVARSPADGYTLLV
                     ISSNNVIDTTLYGTVDFAREIAPIATFGRGYQVLNVNNSLPVKTVPDLISLAKAEPGK
                     LSMASPGIGSAGHVAGEMFKMMTSVNMLHVPYRGSVPALTDLMSGQVQVMFDNLPSSI
                     EHIRAGRIRALAVTPATRSQVLPGVPTIGESVPGYVGGGWSGIGAPARTPTEIVDLLN
                     GEVNAALADPKIKGRLENLGSPVLMMSPAEFRKLVAEETERWAKVIKFANIRPE"
     gene            complement(965..1111)
                     /locus_tag="VM910_02035"
     CDS             complement(965..1111)
                     /locus_tag="VM910_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61365.1"
                     /translation="MACLDPAVARVSLGTVEAPNAKAAEAAAMKEFKLSDEQRRRLEV
                     QERD"
     gene            complement(1270..2529)
                     /gene="ltrA"
                     /locus_tag="VM910_02040"
     CDS             complement(1270..2529)
                     /gene="ltrA"
                     /locus_tag="VM910_02040"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HVI61366.1"
                     /translation="MDKAKPFDIPKREVWEAFKKVKANQGAAGVDGQSIADFEADLSN
                     NLYKLWNRLSSGSYFPPPVRRVEIPKVDGGTRPLGIPTVADRVAQEVARRYLEPRLEP
                     VFHTDSYGYRPGKSAIDAVRQARQRCWRYDWVLDLDVKAYFDSIDWELMLRAVRRHTN
                     QPWVLLYTERWLKAPVQLENGSVVPRMSGTPQGGVISPILANLFLHYAFDTWMARTLS
                     HIPFERYADDAICHCKSAEEAEALWSALADRFAACKLVLHPEKTKIVYCKDANRRGDF
                     PNQSFDFLGFMFRARKALGKGRRAFACFLPAASPKALTFIGRTIRRWTLHHSSDKSLP
                     ILAEMYNPCIQGWINYYGNFYRTQLRSTLKRIDLYVIRWARRKFKRLRRKTKGARDWF
                     NRLRRVNPTLFAHWQLCHGSGRTSGAV"
     gene            4331..4567
                     /locus_tag="VM910_02045"
     CDS             4331..4567
                     /locus_tag="VM910_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61367.1"
                     /translation="MSEVIQALLKCERVSFEPEDVAKLVAGFESAVTELGLKDRNDPV
                     TTQVAKLIIQLARDGHRDPKRLAERATAIVRGST"
BASE COUNT          936 a         1480 c         1401 g         1039 t
ORIGIN      
        1 cgcctgatgg cggaatggct gcagcagcgg ctcggtcaga cattcatagt cgagaaccgg
       61 ccgggtgctg ccgccaatgt cgctaccgat atggtggcgc gctctccggc ggacggttac
      121 acgcttctgg tcatttcgtc gaacaacgtg atcgacacca cgctctatgg caccgtcgat
      181 ttcgcccgcg aaattgctcc gatcgccacc tttggccgcg gctaccaggt tctgaacgtc
      241 aataattctt tgccggtgaa gaccgtcccc gatctcatct cccttgcgaa ggccgagccc
      301 ggcaagctca gcatggcgtc tccgggaatt ggaagtgccg gtcatgtcgc tggcgagatg
      361 ttcaagatga tgacgagcgt gaacatgctg catgtgccgt accgcggctc ggtgccggcg
      421 ctgaccgacc tcatgagcgg gcaggttcag gtcatgttcg acaacctgcc ctcgtcgatt
      481 gagcacatca gggctggcag gattcgtgcg cttgcggtca cgcccgcgac gcgctcgcag
      541 gtcctgcctg gtgtcccgac catcggtgag tcggtgccag gctatgtggg gggcggctgg
      601 tccggaattg gcgcgcctgc ccgaactccg acggaaatcg tcgatctgct caatggcgag
      661 gtcaacgccg cgctcgctga tccgaagatc aaggggcggc tcgagaacct aggcagcccg
      721 gttcttatga tgtcacctgc ggaattccgc aagctcgtcg ctgaggagac cgagcggtgg
      781 gcgaaggtga tcaagttcgc gaatatcaga ccggagtgat gcgcttgagg gcgtcggatc
      841 aacgcttttc gcaagtcgag ttgctgagta gcgcaaacgc gacagtcggt agttcactca
      901 ccaatctaga gcaggcgaat actaatctcg gtttcacttt tgccggcggc gcggccacgc
      961 agagctaatc ccgttcctgc acctcgagcc gcctgcgctg ctcgtcgctc aacttaaatt
     1021 ccttcattgc ggcggcttcg gccgccttcg cgttcggtgc ctcgaccgtg ccgagggata
     1081 cgcgcgcgac cgcaggatcg agacacgcca cgatctcagt tttgcggcct tacgctttgc
     1141 aatatgagcc ttcatgactg ccgttggcct tgttatctgt ccgccataac cactccggtc
     1201 gctgatgtcg agtcgcccgg aggaatctca cccccgggcg ctcccagatc cgtgcatgaa
     1261 cctctcgatt cacacggctc ccgatgttcg gccgcttcca tggcacagct gccaatgagc
     1321 gaatagcgtc ggatttactc ggcgaagccg attaaaccaa tcgcgcgctc ctttggtctt
     1381 gcgacgcagc cgcttgaatt tgcggcgtgc ccagcggatg acatagagat caatcctctt
     1441 caaggtcgaa cgcaactgcg ttcggtagaa gttgccgtag tagttgatcc agccttgaat
     1501 gcacgggttg tacatctcgg ccagtattgg cagggatttg tcgctgctat gatgaagcgt
     1561 ccagcgccgg attgtccggc caatgaacgt caacgccttc gggctggcgg cgggtaggaa
     1621 gcacgcaaag gcacgtcgcc cttttcccaa cgcttttctc gcccggaaca tgaacccgag
     1681 aaagtcgaac gattggttcg ggaaatcgcc acgccggttc gcatccttgc aatagacgat
     1741 cttcgtcttc tcaggatgca gcacaagctt gcaggccgca aagcggtcgg caagcgcgct
     1801 ccataacgcc tccgcctcct cggcgctctt gcaatgacaa atggcgtcat ccgcgtagcg
     1861 ctcgaacggg atgtgggaca aggttctcgc catccaagtg tcgaacgcat aatgcagaaa
     1921 cagattcgcc aggatggggc ttatgacccc tccctgagga gttccagaca ttcgcggtac
     1981 aacgctgcca ttttccagct ggaccggagc cttcagccag cgctcggtat agagcagcac
     2041 ccatggttgg ttcgtatgtc gacggaccgc cctgagcatc agctcccaat cgatgctgtc
     2101 gaagtaggct tttacgtcga gatcgagcac ccagtcgtag cgccaacagc gctgacgagc
     2161 ttggcgcacg gcgtcgatcg ccgatttgcc gggcctgtag ccataggagt cggtgtggaa
     2221 caccggctcc aaacgcggct ccaggtagcg acgggcaact tcctgagcga ctcggtcggc
     2281 gaccgttgga atgcccaatg ggcgcgtacc gccatctacc ttcggaattt ctacgcgtcg
     2341 caccggcggt ggaaagtagc tccctgacga caaccgattc cagagcttgt agaggttgtt
     2401 cgaaagatca gcctcaaagt ccgcaatcga ctgtccatct actccagccg ctccctggtt
     2461 ggccttcacc ttcttgaagg cttcccagac ttcccgtttg gggatgtcga acggctttgc
     2521 tttgtccacg ggctcctccc gttgccagat ggcttcagtt ggcccgtggt cgcggctgag
     2581 caacgcagcc ccttcggtcc agctccatta cagagccttt atccctaata cgagctgctc
     2641 cgtccctgtg ctccgcatcg gtactcttgt cctcgcggtt ttggccgctt gagacttctc
     2701 cctttgcatc ggagcgacag gttctcacgt tccgtacaag agcctggttg agcttcgcgc
     2761 cgtctacatg ccggatgccg ctcgggcagt cttcagggtc cccccgagct gatcccggag
     2821 gcagggtcag cccccggttt tgacatcgtc taatccgctt tcgacacttc atcgacggtt
     2881 cgcttgcgct cgcctctctc aaccttgcct gccggaatct tgtcccggcg tttccgcagc
     2941 gctcaccacc attgcttttg acaacagcag cttgcggtgg cttgaggtca acacctgttt
     3001 gccgacctcg aagggccctc ctccatctct cgtacagttg cgcatggccg tttggaccgg
     3061 cgatgctcgt gacacacgac ccaaagcaga caaaggtagg actgaaatct cgcagcgcag
     3121 cagtctcctc cgccaacgtg gtgtgcgaac ttacgctcga tctcgtcgag gcgaggcaag
     3181 ggcgagtgag aggctgtcgg cggtggtcat gggttgccgc atcgaacacc cggaaggatg
     3241 agaccccagc cacccacttc tggctagggc ctcacttggc agacggcccg ctctgtgtgg
     3301 gagagtggca ggagacgtta tctctcccac atcctagcgc cggacacccc gttatgctta
     3361 tccgcgccaa agatttgctc cttgagtcca ttgccacctt gggccaccca ggcgtcctcc
     3421 ggcttccatc ggtggacctg cccgcacgcc aggcaccgca ggggtagagc aatgggcggc
     3481 aaggtttcga aaatgaccat atcggtcgtc aggtcggtcg ctactggccg ctttgtctgc
     3541 gggcatctga ttacgacgtc actcacggct aacccttcaa cagaagtgca caggcggtcg
     3601 gaaccgaaaa ttttgatgag acggcgcggt gtggcagcaa aaatggtctg attcttgccc
     3661 gagtaaggat atcaaatccg cagggctaat ccagcggcta gaccttgcta ggtggcagat
     3721 gatggcgaga accacttcgg gcttggtaac ggagcatgac aatcccgcca gcttcacctg
     3781 ttcgcaatgt ggggcgcact acaaggtagt gcgagtaaag acggaaaccc aatctcttga
     3841 ccgcttgcta cattgcaagg tttgcaagca gccgctcgcc gctacggatg gcgaagatat
     3901 cctaaagtac ttcctcataa gccggcccgc caggaaatcc caagcgggtc ctcgtcttta
     3961 agacggccag acatccttct gccgtcaccg caatctctcc acctcgatca ttatctccgg
     4021 cgtcgaattg ttcagcgcaa cctgcctgat ctggctgtcc acggcatcac ttgcgggcgc
     4081 ttcgtggcgc aagcggccgc gtgcgttgtc atctaagttt cttgttccgc ttcttacaat
     4141 cggggcgccg gccttcggtg ggaacccctt aggaaggccg cgtggtcctc ggggtcgttg
     4201 tggccaactg ctccggggac catcccccca cgcgcgaatg ggcactggcg gcgagggcag
     4261 gctccaccac ggtcatttcg tggggcggcc ccgccccgaa cgtgggaatc acgccaaaga
     4321 gggtagcgcg atgtcggaag tgatccaggc gttgcttaag tgcgagaggg tttcgtttga
     4381 gccagaagat gtcgccaagc tcgttgcggg ctttgaatcg gcagtaacag agcttggcct
     4441 caaggatcgc aacgacccgg tgacgactca ggtcgctaag ctcattattc aacttgccag
     4501 ggacggccac cgagatccaa aacgcctagc ggaaagggcg accgcgattg tgcgcggctc
     4561 tacctagccg ccctcgcggt cggcttatgg cacaaagcgg acatgctgga gcgtccatcg
     4621 aatgtccgct ttcgggggta aagcggacat gaggcctgcg atcggcgtat agaattgagg
     4681 ccgacgcggg gacaatgaat ttttctgtcc agaatagccc tagtccgcca attaaattca
     4741 tttcggtgga tgcgcgccct ctttcgacag gactagtttc gcttcgagcc ggctgtcctg
     4801 aaacaatacc tgcccactat gaccggcacc cgccctgtct tggcaaaaat tgcagt
//
LOCUS       DATLJT010000093         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2760965,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000093 DATLJT010000000
VERSION     DATLJT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760965"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1379)
                     /locus_tag="VM910_02050"
     CDS             complement(102..1379)
                     /locus_tag="VM910_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI61368.1"
                     /translation="MTSTDASARVAPLTAKRESSPVDAKPSTRSPKDQSRPFIKPLLK
                     SHRELKRPPFDCIALVLQGGGALGAFQAGVYQGLAEANLHPDWVAGISIGAINAALIA
                     GNAPEARLEKLRAFWEQVTTQLLFDPFGIAHYWLRGDTGHVFLNRFRAGATLLEGAPG
                     FFKPRIPPPYFAPPGTVEATSWYDTAPLRSTLERLVDFDRINAGEMRFSVGAVNIRSG
                     NFVYFDSTSHKIGPEHIMASGALPPGCPAVEVDGELYWDGGLVSNTPLQWVVAGDTRQ
                     DTLTFQVDLWSARGDLPSDLNDVSARQKEIQYSSRTRANTDHFKQIQCMRVALADMLQ
                     KIPREMLDTEDGKLLQSVADRNVYQIVHLIYRSKNYEVQSKDYEFSRNSMIDHWNAGY
                     NDAIRTLRHPEALQRPTDLAGVATFDVAIDGRE"
     gene            complement(1480..1791)
                     /locus_tag="VM910_02055"
     CDS             complement(1480..1791)
                     /locus_tag="VM910_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61369.1"
                     /translation="MLWTIADKVPALDVESSCRAAAKMGDSLSLDATLRQCLGDEKSA
                     REELEKQWTQFPAALRQRCVTTTETGGDPSYVEVLVCLQMGRDAAQTEDPLGGGRQDK
                     K"
BASE COUNT          348 a          622 c          644 g          397 t
ORIGIN      
        1 gccgggttcg tcagcggcat ggcgaatgcg cgtgccttga cctctgcttc cttcatgctg
       61 tgctccttct caattcggtg ggtctgggca tcgcgttgcc gtcattcgcg gccgtcgatg
      121 gcgacgtcga atgtggcgac cccggccaga tcggtcggcc gttgcagcgc ttccggatgg
      181 cgcaacgtgc ggatcgcgtc gttgtatccc gcgttccagt ggtcgatcat gctgttgcgg
      241 gagaactcgt agtccttcga ctgaacctca tagttcttcg agcggtaaat gaggtgcacg
      301 atctggtaga cgttgcgatc cgcgacggac tgcagcagct tgccgtcctc ggtgtcgagc
      361 atctcgcgtg gaatcttttg cagcatgtcg gccaatgcca ctcgcataca ctggatctgc
      421 ttgaaatgat ccgtattggc ccgcgtcctg ctcgaatatt ggatctcttt ctgcctcgcg
      481 ctgacatcgt tgagatcgct cggcaagtcg ccgcgggcgc tccacaggtc gacttggaac
      541 gtgagcgtat cctggcgggt atcgcccgcc accacccatt gcagcggggt gttggagacg
      601 agcccgccat cccaatagag ttcgccatcc acctcgacgg ccggacagcc gggcggcagc
      661 gcgccgctcg ccatgatgtg ctcgggcccg atcttgtggc tggtcgaatc gaaatagacg
      721 aagttgccgc tgcgaatgtt gacggcgccg acgctgaagc gcatctcgcc ggcattgatg
      781 cggtcgaagt cgacgagccg ctcgagcgtg ctgcgcaacg gcgcggtgtc gtaccagctg
      841 gtggcctcga ccgtgcccgg cggcgcgaag taaggcggcg gaatgcgcgg cttgaaaaag
      901 ccgggagccc cttcgagcag ggtcgctccg gcgcggaacc gattgaggaa gacgtgaccg
      961 gtgtcgccgc gcaaccagta atgggcgatg ccgaaggggt cgaacagaag ttgggtcgtg
     1021 acctgctccc aaaaggcgcg cagcttttcg agccgcgcct cgggtgcgtt gccggcgatc
     1081 aaggccgcat tgatcgcgcc aatggaaatt ccggcgaccc aatcggggtg caggttcgcc
     1141 tcggctaacc cctgatacac gccggcctgg aaggcgccga gcgcgccgcc gccttgcaat
     1201 accaaggcga tgcagtcgaa cggcggccgc ttcaattcgc ggtggctttt cagcagcggc
     1261 ttgatgaagg gccggctttg atctttgggc gaccgcgtcg acggtttggc atcgacgggt
     1321 gaggactcgc gcttcgcggt taaaggcgcg acgcgggccg aagcatcagt ggaggtcatg
     1381 caacgacgat ccgttttcac gttccagcgc tctcgagttc gataggcaaa gcggattgat
     1441 gatcggccga tcaaatcacg aatacgtgca tgatttgtgt catttcttgt cttgccgacc
     1501 gccccccaaa gggtcttccg tctgcgccgc gtcgcgcccc atctgcaggc agacgagaac
     1561 ttccacgtag ctcggatcgc cgccggtttc ggtggtggtc acgcaacgct gacgcagcgc
     1621 cgccggaaac tgcgtccact gcttttccag ctcctcacgc gcgctcttct cgtcccccaa
     1681 gcattggcga agcgtcgcat ccaagctcaa gctgtcgccc attttcgccg ccgcacggca
     1741 gctggattcg acgtcgaggg cagggacctt gtcggcgatg gtccacagca atgtaccacc
     1801 gagcgcgaca agcgacgctg atggaaacat tcgggtctcc acttttctcg cgggccggct
     1861 gcgcaaccga aaaatgtgcg ccgacggtga aacgtcggcg gtgcgccgcc ccagccggca
     1921 gcggggcata gcccggaggc gcaggccggt caaccggctt cgttgcatca aattagagca
     1981 tcgcgtagcg ctgtcggctc gccgtgcaaa c
//
LOCUS       DATLJT010000094         6204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2531156,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000094 DATLJT010000000
VERSION     DATLJT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531156"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VM910_02060"
     CDS             complement(<1..465)
                     /locus_tag="VM910_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61370.1"
                     /translation="MSATDTPFERASDPYRIGRSRDWVSTGAGRGYVYATGHGEGGPA
                     SKRVIVGYGFWIFLLSDIVMFSAFFAAHAVLQTATAGGPSGRDLFNWWSIVAQTAFLL
                     VSSFTCGLSAVATEARDQLWTQVALLVTGLFGLGFLVLEGRDFAGMVMQGAGP"
     gene            complement(462..2468)
                     /locus_tag="VM910_02065"
     CDS             complement(462..2468)
                     /locus_tag="VM910_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI61371.1"
                     /translation="MFGKLTWDAIPWSQPIPLVAGSVVVLVVLAVLAWVVLKGYLPYL
                     WREWITSVDHKRIGVMYTLLGLVMLLRGFIDAIMMRSQQAIAFRAAGYLPPEHYDQIF
                     SAHGTIMIFFAAMPIIIGLMNFAVPLQLGVRDVAFPTLNSTSFWLTATGALLVNISLV
                     IGEFARTGWLVYPPLSELPYSPGVGVDYYLWALLISGVGTLMTGINFVTTILKIRAPG
                     MSYLRMTIFCWTALAANLLIVAAFPILTATLGMLALDRYLGFHFFTNDAGGNMMMFIN
                     LIWTWGHPEVYILVLPAFGIFSEVISTFSGKPLFNYRSMVAATMFICLVSYLVWMHHF
                     FTMGAGADVNAFFGISTSIIAVGTGVKIYNWLFTMYGGRIRFTTPMLWSVGFLVTFTI
                     GGMTGVLLAVPPADFLLHNSLFLVAHFHNVIIAGVIFAAFAGVTYWFPKAFGFKLDEG
                     WGKAAFWLSLLGFYVVFVPLYVAGLLGMTRRLQHYDVAAWRPWVLVAGIGIIIMMAAA
                     ACQVVQFVVSIRRREQLRDETGDPWDGRSLEWATASPPPPFNFAVLPNVQGAEPYWGI
                     KQRAIETQQLSAEPRYEPIEMPRNSPTGFITAFFSTLTGFALIWHIWWLVGLGLVAAY
                     AVFVWFAWRDAVEYSIPAEEVARYDRERRRAREQWLAQHAGQPA"
     gene            complement(2470..3369)
                     /gene="cyoA"
                     /locus_tag="VM910_02070"
     CDS             complement(2470..3369)
                     /gene="cyoA"
                     /locus_tag="VM910_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HVI61372.1"
                     /translation="MKRHPLPWRTSAALILLLALSGCSGGVLDPQGPIGAANAKILLN
                     ALGIMLVIVVPTIIAILIFAWWFRASNPRARYRPDFVYSGAIEMVVWGIPILVIMFLG
                     GVIWIGSHDLDPFKPISSPEKPVEVQVVSLDWKWLFIYPEQGVASVNELVVPAGVPVH
                     FSLTSASVMNMFFVPQLGTMIATMNGMVTQLHLAADKPGEFYGQSAQYSGGGFSDMNF
                     TVRAVPRDAFAQWVAGVRQSGSALDRSSYLALCQQSSNVRPFTYRAVDAGLFDAVVNQ
                     QLPPCPGPNIERAGATVRPRPER"
     gene            complement(3659..4870)
                     /locus_tag="VM910_02075"
     CDS             complement(3659..4870)
                     /locus_tag="VM910_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61373.1"
                     /translation="MSTRSQLFCLATASAVCFGTGAFAQSTVKIGELNSYKAQPAFLE
                     PYKRGWELAVEDINAKGGVLGKKLEVISRDDGASPGDAVRVANELVTREGVNIIAGTF
                     LSHIGLAVTEFAGKEKVFFLAAEPLTDKITWQNGNKYTFRLRPSTYMQVAMLMPDAVA
                     AKKKRWAVIYPNFEYGQSAATWFKEMLRKAQPDVEFVTEQAPPLGKVDAGAVVQAIDD
                     AKPDAIFNVLFGGDLAKLVREGTTRGTFKNRVVVSLLSGEPEYLDPLKDEAPVGWIVT
                     GYPWDAINTPAHKAFVAAYQKRWNDYPRLGSVVGYDTINSLAAGIAKAGSTDTDKLVA
                     AFRGLKVEGPFGPFEFRANDHQATMGAFVGKIALKDGKGTMSDFKYVDGASVMPSDEE
                     VKKLRPAAAMN"
     gene            complement(5153..5965)
                     /locus_tag="VM910_02080"
     CDS             complement(5153..5965)
                     /locus_tag="VM910_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI61374.1"
                     /translation="MDLKLEGKTALVTGGSAGIGKGIARALAKEGVDVAICARRKEPL
                     EQAAAEIARETNRRIVPIPADLSKDADAKNFVAQGHKALGRVDILVNNAGSSPGGVIE
                     HLSETDWEQSLQLKFMGYVRCLRYVLPIMVKQGGGRVVNLIGNDGVKPSYWEIAPGAA
                     NAAGQNLTLSLAGQYGKHNVSFCAVNPGPVRTERWAGLVAAMSRDMKLPYEEADKLAP
                     SSIPLGRIAEVEEVANLVVMLASPLMHMVNGTQIEVDGGQEKSLMDRLRDRR"
     gene            complement(6004..>6204)
                     /locus_tag="VM910_02085"
     CDS             complement(6004..>6204)
                     /locus_tag="VM910_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI61375.1"
                     /translation="VIVGDEDDLCLDGSVFIKRTAPTAALLVVPRAGHTINSEEPAAV
                     NAALAELFSAAEAGRWLAHKSA"
BASE COUNT         1180 a         1969 c         1984 g         1071 t
ORIGIN      
        1 ggggccggcg ccttgcatca ccatgccggc aaaatcccgc ccctcgagca ccaggaagcc
       61 cagcccgaac aaccccgtca ccagtagcgc gacctgcgtc cacagttgat cgcgtgcctc
      121 ggtcgctacg gccgatagcc cgcaggtgaa gctcgagacg agcaaaaacg ccgtttgcgc
      181 cacgatgctc caccagttga acaggtcgcg cccggaaggc ccgcccgccg tggccgtttg
      241 cagcacggca tgcgcggcga agaaggcgga gaacatcacg atgtcgctga gcagaaagat
      301 ccagaagccg tagccgacga tcacgcgctt cgaggccggg cccccttcgc cgtgcccggt
      361 tgcataaacg taccctcggc cagcaccggt gctgacccaa tcacggctgc gcccgatccg
      421 gtacggatcg cttgcgcgct cgaacggggt gtccgtcgcg ctcatgccgg ctggcccgcg
      481 tgttgcgcga gccattgctc gcgggcgcgc cggcgctcgc ggtcgtagcg ggcgacctcc
      541 tccgccggaa tcgagtactc cacggcgtcg cgccacgcga accacacgaa cacggcatac
      601 gcggcaacga ggcccaggcc caccagccac cagatgtgcc agatcagcgc gaagccggtg
      661 agggtggaga agaacgccgt gatgaatccg gtcgggctgt tcctcggcat ctcgatcggc
      721 tcatacctcg gctctgccga taattgctgc gtctcgatcg cgcgctgctt gatcccccaa
      781 tagggctccg cgccctgcac gttcggcagc accgcgaaat tgaagggggg tgggggcgat
      841 gcggtcgccc attcgagcga gcggccgtcc cagggatcgc ccgtctcatc gcgcaattgc
      901 tcgcgccggc ggatgctcac gacgaactgc accacctggc aggcggcggc cgccatcatg
      961 atgatgatgc cgatcccggc gacgagtacc catgggcgcc acgccgccac gtcgtagtgc
     1021 tgcagacgcc gcgtcatgcc gagcaggccg gccacataga gcggcacgaa caccacgtaa
     1081 aagccgagga gcgagagcca gaacgcggcc ttgccccaac cttcgtcgag cttgaacccg
     1141 aacgccttgg ggaaccaata cgtcacgccg gcgaaggcgg cgaatatcac gccggcgatg
     1201 atgacgttgt ggaaatgcgc gaccaggaac aggctgttgt gcagcaagaa gtcggcaggc
     1261 ggcacggcaa gaagcacgcc ggtcatgccg ccgatcgtga atgtcacgag aaatcccacc
     1321 gaccacagca tcggcgtggt gaaacggata cggcccccgt acatcgtgaa cagccagttg
     1381 tagatcttca ccccggtgcc gaccgcgatg atgctggtgg agatgccgaa gaaggcattg
     1441 acgtcggcgc ccgcccccat agtgaagaaa tggtgcatcc agaccaagta ggagacgagg
     1501 cagatgaaca tggttgctgc gaccatcgag cggtagttga acaacggctt gccggaaaac
     1561 gtggagatca cctcggagaa gatgccgaac gccggcagca ccaggatgta gacctcggga
     1621 tggccccagg tccagatcag gttgatgaac atcatcatgt tgccgccggc gtcgttggtg
     1681 aagaaatgga agccgaggta gcggtcgagc gcgagcatgc ccaaggtggc ggtgaggatc
     1741 ggaaaggcgg cgacgatcaa gagattggcg gcgagcgcgg tccagcagaa gatcgtcatg
     1801 cgcaagtagc tcatgccggg cgcgcggatc ttgagaatcg tggtgacgaa gttgatgccc
     1861 gtcatcaatg tgccgacccc cgagatcagc agcgcccata ggtagtaatc gacaccgact
     1921 ccgggcgagt agggcagctc cgacaacggc ggatacacca gccagcccgt gcgcgcgaac
     1981 tcgccgatca ccagcgagat gttaaccagg agcgcgccgg tcgcggtgag ccagaagctt
     2041 gtcgaattca gcgtggggaa ggcgacatcg cgcacgccga gctgcagcgg caccgcgaaa
     2101 ttcatcaacc cgatgatgat cggcatggca gcgaaaaaga tcatgatcgt gccgtgcgcg
     2161 gaaaagatct ggtcgtagtg ctcgggcgga aggtaaccgg cggcgcgaaa tgcgatcgcc
     2221 tgctgcgagc gcatcatgat cgcgtcgatg aaaccgcgca gcagcatgac caggccgagc
     2281 agggtgtaca tcaccccgat gcgcttgtgg tcgacgcttg tgatccactc gcgccagagg
     2341 taaggcaaat agcctttgag cacgacccac gcgaggaccg cgaggacgac caacaccacg
     2401 accgagccag caacgagcgg gatcggctgg ctccatggaa ttgcatccca ggtcagtttg
     2461 ccgaacatgt cagcgctccg gccgcggacg aaccgtcgcg cccgcgcgtt cgatgtttgg
     2521 ccccggacag ggtggaagct gctggttcac gaccgcatca aacaagcccg catcaaccgc
     2581 gcgataggtg aaaggacgta cgttgctgct ttgttggcac agcgcgagat aactcgatcg
     2641 gtccaatgcc gatccactct ggcgcacgcc cgcaacccat tgcgcgaaag cgtctcgggg
     2701 caccgcgcgc accgtgaagt tcatgtcgga aaagccgccg ccgctgtact gcgcggattg
     2761 gccatagaac tctccgggct tgtcggccgc caggtggagc tgcgtgacca ttccgttcat
     2821 cgtggcgatc atggtgccga gctgcggcac gaagaacatg ttcatcacgg aggccgacgt
     2881 cagcgagaaa tgcaccggca ccccggcggg aacgacgagc tcgttcacgc ttgcaacgcc
     2941 ctgctcgggg tagatgaaca gccatttcca atcgagcgag acgacctgca cctcgaccgg
     3001 tttctcgggc gagctgatcg gcttgaaggg atcgagatcg tgtgatccga tccagatcac
     3061 gccgccgagg aacatgatga ctaggatagg aatgccccag acgaccatct cgatcgcgcc
     3121 ggagtagacg aagtcgggac ggtagcgcgc ccgcggattg gatgcgcgga accaccaggc
     3181 gaagatcagg atcgcaatga tcgtcggcac cacgatcacc agcatgatgc ccaaggcgtt
     3241 gagcaggatt tttgcgttcg ccgcgccgat cggcccttgc gggtccagca cgccgcctga
     3301 acagccgctc agcgcgagga gaagaatcag cgcagccgac gttcgccagg gcagggggtg
     3361 ccgtttcaaa agcaatgctg cccacgatga catggtgtct ggcggccgca tctttgtcag
     3421 gctttgctca accggaacta ccggaaaccg gctcgaacct caaccgtcgg cattcaattt
     3481 gtctatgatt cctcatgccc tctgccctgc ggcaggggca cattggtgcg tccatggatt
     3541 tggagaggtg aggcgcttga tacgatcccg ctcatccatc cttcggcgtt gatgcattcc
     3601 accgcgcgcg ccgtcccacg tcttgggcgc ggaacgagcc gatccagcga cgctgcgatc
     3661 agttcatcgc cgccgccggt cgcagcttct tcacctcctc gtccgatggc atcacgctcg
     3721 cgccgtcgac atatttgaag tcggacatcg tccccttgcc gtccttgagc gcgatcttgc
     3781 cgacaaacgc gcccatggtg gcctgatggt cgttggcgcg gaattcgaac ggcccgaacg
     3841 gaccttcgac cttgagcccg cggaacgcgg cgacgagctt gtcggtatcg gtcgagcctg
     3901 ccttggcaat gccggcggcg agcgaattga tggtgtcgta gccgaccacc gaacctagcc
     3961 gcggatagtc gttccagcgc ttctgatagg cggcgacgaa cgccttgtgc gccggcgtgt
     4021 tgatcgcatc ccaaggatag ccggtgacga tccatccgac gggcgcttcg tccttgagcg
     4081 gatcgagata ttccggctcg cccgagagca ggctgacgac gacccggttc ttgaacgtgc
     4141 cgcgggtcgt gccctcgcgc accagcttgg cgaggtctcc tccgaacagc acgttgaaga
     4201 tggcgtcggg tttggcatcg tcgatcgcct gcacgaccgc gccggcgtcg accttgccga
     4261 gcggcggggc ctgctcggtc acgaattcga cgtctggctg ggcttttctc agcatctcct
     4321 tgaaccaggt cgcggccgac tggccgtact cgaagttggg gtagatcacg gcccagcgtt
     4381 tcttcttcgc cgcgaccgcg tcgggcatca gcatcgcgac ctgcatatag gtggagggcc
     4441 gcaggcggaa cgtatacttg ttgccgttct gccaggtgat cttgtcggtc agcggttcgg
     4501 ccgcgaggaa gaacaccttc tccttgccgg cgaactcggt gaccgcgagg ccgatatggg
     4561 agagaaacgt gccggcgatg atgttcacgc cctcgcgggt gacgagctcg ttggcgacgc
     4621 gcacggcatc gccggggctc gcgccgtcgt cgcgcgaaat cacctcgagc ttcttgccga
     4681 gcacgccgcc cttggcgttg atgtcctcaa ccgcgagctc ccagcctctc ttatagggct
     4741 cgaggaatgc cggctgcgcc ttgtagctgt tgagctcgcc gatcttgact gtgctctgcg
     4801 cgaaggctcc cgtgccgaaa caaacggcgc tcgcggtggc gaggcagaag agctgggatc
     4861 tggtgctcat gcgatgctcc attccataag atttgcaagt cgcgcgctag ttcgttcgtg
     4921 cataaagtag tcgaaccggc ccgaaggctt gtcaagatcg cttgatcgcg cacctgcttg
     4981 gtaatgcttc tgtaagcggg gacccagcct ttggcactgg attcccagca gaagcgcgtt
     5041 tacgcgcgtc catagcccgc gaagacgggc gtcaacgccc ttaaggacgc gctatgcgcg
     5101 ggaatgagcg gagagttttg ctgcgcgggc catcgcgcac cccagggaca cctcacctgc
     5161 ggtcgcgcag gcgatccatc agcgacttct cctggccgcc gtcgacctcg atctgcgtgc
     5221 cgttgaccat gtgcatcagc ggcgaagcca gcatgaccac gaggttcgcg acctcttcga
     5281 cctcggcgat acggccgagc gggatcgagg acggagcaag cttgtcggcc tcctcgtagg
     5341 gcaatttcat gtcgcgtgac atcgccgcga ccaggccggc ccagcgctcg gtgcgcaccg
     5401 ggccgggatt gaccgcgcag aagctgacgt tgtgcttgcc gtattgaccg gcgagcgaca
     5461 gcgtgaggtt ctgcccggcg gcgttggcgg cgccgggcgc gatctcccaa taggacggct
     5521 tgacgccgtc attgccgatc aaattgacca cgcggccgcc cccttgcttg accatgatcg
     5581 gcagcacgta gcgcaggcag cgcacgtagc ccatgaattt gagctgcagc gactgttccc
     5641 agtcggtctc ggacaaatgc tcgatcacgc cgccgggcga ggagccggca ttgttgacca
     5701 ggatgtcgac ccgaccgagc gccttgtggc cctgcgcgac gaaattcttg gcatcggcgt
     5761 ccttgctgag gtcggcgggg atcggcacga tccggcggtt ggtttccctt gcgatctcgg
     5821 ccgccgcctg ctcgagcggc tccttgcgcc gcgcgcagat ggcgacatcg actccttctt
     5881 tcgccagcgc gcgggcgatg cctttgccga tgcccgcgct gccgcctgtg accagcgcgg
     5941 tcttgccctc gagcttaagg tccatgtcgg tctcctcgtg cgattttccc tcgtgggttg
     6001 ggctcaagcc gatttatgcg cgagccagcg gcccgcctcc gcggcggaga agagctcggc
     6061 gagcgccgcg ttcaccgccg ccggttcctc gctgttgatg gtgtggcccg cacgcggcac
     6121 caccagaagc gctgcggtcg gagcggtgcg cttgatgaat acgctgccgt cgagacaaag
     6181 atcgtcctcg tcgccgacga tcac
//
LOCUS       DATLJT010000095         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1612144,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000095 DATLJT010000000
VERSION     DATLJT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612144"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1277)
                     /locus_tag="VM910_02090"
     CDS             complement(294..1277)
                     /locus_tag="VM910_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI61376.1"
                     /translation="MTELTRRRIVAAAGATAAAGALAPFAPPAQAAAPLARSQAPGFY
                     RCKVGEFEVTVVNDGARAIPLPAQFVRKVGNEEVLAAAEAAYMPKGSIIAPFNPVVVN
                     TGAKLVLIDTGYGPGLGPTVGLLPSTLAAAGFDPKAIDIVLISHMHGDHILGLKTPDG
                     ALAFPNAEIKVPVIDWAFWMNDDNMSRAPEGFTKTSFGFNRKIFSNLADKVTRYEWGR
                     EVAPGITAVETSGHTPGHTSFVIASGSSQLFFQGDVTNVPDLFLRNPDWQVMFDHEPE
                     KAVATRRRVYDMASADKLLVSGYHFPFPGLGYIEKAGSGYRLVPAAWNPVI"
     gene            complement(1560..1967)
                     /locus_tag="VM910_02095"
     CDS             complement(1560..1967)
                     /locus_tag="VM910_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61377.1"
                     /translation="MSVDAPRILPGFSRDFIAKRVAFAAVVLVATVAASYAWATSWAA
                     RAQELAPLEALSLQFGEMNGVAYYTVQGPSYRVVATLATEGGTPMRFEGSLLPGQKLV
                     VSIPDSASAKAQTIELVRRGNRLRVEANPMEGE"
     gene            complement(2143..2550)
                     /locus_tag="VM910_02100"
     CDS             complement(2143..2550)
                     /locus_tag="VM910_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI61378.1"
                     /translation="MVRRNGKAFARIQWDTWSPPGWFSDADFERTAKSFENPDWPEIT
                     WHSYSVRWGEADKDPRYCALDRRVREAQSIAIPTLMIQGGSDAVTLPDSTAGKDRFFT
                     GGYARHVLPAVGHFPTREAPDAVDKLVIDFLRA"
     gene            complement(2596..3018)
                     /locus_tag="VM910_02105"
     CDS             complement(2596..3018)
                     /locus_tag="VM910_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI61379.1"
                     /translation="MPFATTATLNIAFETGGPVDGDPMLLLHGWPDDVRTYDRVVPAL
                     HAAGFRTVVPYLRGFGDTSFLSKDTQRSGEMIAMGQDAIDLADALSLQTFAVIGHDWG
                     ARIAYVLAIVVPHRVRRMVTMSVGWEPGDLPTPSLKQA"
     gene            complement(3081..3344)
                     /locus_tag="VM910_02110"
     CDS             complement(3081..3344)
                     /locus_tag="VM910_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61380.1"
                     /translation="MRNHGRFEEPVSVTLKRDEAIVLLWYLTRELWNEHQRNLRASFV
                     HPAEAHSLKALLQELIPPLMDTGAPDAAGIEIAARDHLLRRFE"
BASE COUNT          672 a         1111 c         1109 g          615 t
ORIGIN      
        1 ttccgactga aatggtagca catcacgcca tgggacgaga ccactgcact gcggattttg
       61 atccgggttg cgccgcaacg ggtcaggagt ggaaatcgca cgtcacctag tcaatgtccg
      121 ctttaccccc gaaagcggac atcgagcgac acgacgggca tgccgctttg tgccaaaagc
      181 ggacattcgc attggagcaa atagcttcgc tattcgacca tctcgtcggc gcgggcgagc
      241 atcatcgctg ccacgcgaac cgcggttcgc catccgacgg cgaaccatca cgttcaaatc
      301 acagggttcc acgccgccgg gacgagccga tagccgctgc cggccttctc gatgtagccg
      361 aggccgggga acgggaagtg atagcccgag accaggagct tgtcggccga agccatgtcg
      421 tagacgcgcc gacgcgtcgc cacggccttc tccggctcgt ggtcgaacat gacctgccaa
      481 tcggggttgc gcaggaacag gtcgggcacg ttggtgacgt cgccctggaa gaacagctgc
      541 gacgaccccg atgcgatgac aaaggaggtg tgcccgggcg tgtggccgct ggtctcgacc
      601 gcggtgatac cgggagcgac ttcgcggccc cattcgtagc gcgtcacctt gtccgcgagg
      661 ttggagaaaa tcttgcggtt gaatccgaac gaggtcttgg tgaagccctc cggcgcccgg
      721 ctcatgttgt cgtcgttcat ccagaacgcc cagtcgatga cgggcacctt gatctcggca
      781 ttggggaagg cgagcgcccc gtcgggggtc ttgagcccga ggatatggtc gccgtgcatg
      841 tgcgagatca ggacgatgtc gatcgccttg ggatcaaagc ccgcggcggc caaggtcgag
      901 ggcagcagcc cgaccgtcgg acccaggccg gggccatagc cggtgtcgat cagcacgagc
      961 ttggcgccgg tgttgacgac gaccggattg aacggcgcga tgatgctgcc cttgggcata
     1021 taggcggcct cggcggccgc gagcacctcc tcattaccga ctttgcgcac gaactgagcg
     1081 gggagcggaa tggcgcgcgc gccgtcgttg acgacggtga cttcgaactc gccgaccttg
     1141 cagcgataga agcccggcgc ctggctgcgg gcgagcggcg cggcggcttg cgcgggtggg
     1201 gcgaatggcg cgagtgcgcc ggcggcggcg gtcgcgccgg cggcggcaac gatgcggcga
     1261 cgggtcaatt cggtcatggc gtcctcccga aagcagcgcc tcgcgcccgc ggccatggcg
     1321 gggggcggcg ctcgccaatg ctacccattc agggcgcaat acagaaggcc gtcccaagac
     1381 ggatcgtccc aagacggatc atggacgcat cgtgcaggaa tgccgagccg cgcgcctgcc
     1441 ctttgtgcca atagcgtccg aactgtgaag ccttgcatcc gggcggcctg atgatcgatg
     1501 gcgcacgcat cggcgatgca aagagacgca tggcggtgca atgacaagcc ttgcgatgcc
     1561 tactctccct ccatcgggtt cgcctcgacc cgcaggcggt tcccccgacg gacgagttcg
     1621 atggtctgcg ccttggcgct cgcgctgtcc gggatcgata ccaccagctt ctggccgggt
     1681 aacaggctgc cctcgaaccg catgggcgtt ccgccttcgg tggcaagcgt tgcgaccacg
     1741 cggtagcttg gaccttgcac ggtgtaatag gccacaccgt tcatttcgcc aaactgcagg
     1801 ctcagcgctt ctaaaggggc aagctcctgc gcgcgtgccg cccaagaggt cgcccaagcg
     1861 taagacgcgg cgaccgtcgc gaccagaacg acggcggcga aggccactcg cttggctatg
     1921 aaatcccggg agaacccggg gagtatccgc ggggcatcga cagacatttg tccaacctcc
     1981 actagctccg acaagcgtgc gtaggtcggg atataggtgt atatgcacgt ttatccagcg
     2041 atgcgcacgg aaacgtgact aagcccagcc cgtgggctga tccatggcaa ccccggcccg
     2101 cgcgaacgga actgcgcgca ccccgcaaac tccggctctg tgctaagcgc gcagaaagtc
     2161 gatcaccagc ttgtcgaccg catccggggc ttcgcgggtc ggaaaatggc ccacagccgg
     2221 aaggacatgt cgcgcataac cgccggtaaa aaaccggtcc ttgcctgcgg tcgaatccgg
     2281 caaggtcacg gcgtcggatc cgccttggat catgagggtc ggaattgcga tcgactgcgc
     2341 ctcacgcacg cgacggtcga gagcacaata acgtgggtcc ttgtccgcct cgccccaacg
     2401 tacgctatag gaatgccatg taatttccgg ccagtccggg ttctcgaacg atttcgccgt
     2461 ccgctcgaag tccgcatcgc tgaaccaacc gggcggactc caagtgtccc actgaatgcg
     2521 cgcgaaggct ttgccgttcc ttcgtaccat ttgacgcccg cgctcggtcg ccatgaacca
     2581 ctgataccaa tacgcctacg cctgtttgag gctcggcgtc ggcaaatcac ccggctccca
     2641 gccgaccgac atcgtcacca tccgccgcac gcggtgcgga acgacaatgg ccagcacgta
     2701 ggcgatacgc gcgccccagt catggccgat aactgcgaac gtttgcagac taagtgcatc
     2761 ggcaagatcg atggcgtctt ggcccatcgc gatcatctcg ccggaacgct gagtatcctt
     2821 cgacagaaac gaagtatcgc cgaagccgcg cagatacggc acgacagtgc gaaatcctgc
     2881 cgcgtgcaag gctggcacga cgcgatcata ggtgcgcaca tcgtcaggcc aaccatgcaa
     2941 gagcagcatc ggatccccgt cgaccggccc tccggtttca aaagcaatat tcagcgttgc
     3001 agtggtggcg aacggcatgc ctctaaaact ctcgacgcgt catgctcgtt cccgcgcggt
     3061 tcttgagcat gccggcgcga ttactcgaac cgccgcagga ggtgatctcg agccgcgatc
     3121 tcgattccgg cagcatcggg tgcaccggta tccatgagag gtgggattag ctcctggaga
     3181 agcgctttca agctgtgcgc ttcggcgggg tgaacaaagc tcgctcgcag gttcctctgg
     3241 tgctcgttcc aaagttcgcg cgtcaaatac cacagcagca cgatcgcttc gtcccgtttt
     3301 aatgtaacgg agaccggttc ttcgaaacga ccgtggttgc gcatgggctg ttcctattca
     3361 atcgccgcgt cggcgcggcc gagccggaca cgaccccgct tggttcgttg cttgcagccg
     3421 tcgcagcctg cgtcaacgca cgcggagatc gaagcaggag ttgccgctgc gtgcgtatcc
     3481 gcgcgggcat ttcccgacct tcgggac
//
LOCUS       DATLJT010000096         6286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_923154,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000096 DATLJT010000000
VERSION     DATLJT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923154"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VM910_02115"
     CDS             <1..359
                     /locus_tag="VM910_02115"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61381.1"
                     /translation="IGELAEASVASLTVGQLKKLELARALATEPVLLLLDEVMAGLTP
                     PEIKEMMTVIGSLPKRGITVVWVEHVMMAIMNVAERIVVMHQGQIIAQGTPADVSKNP
                     AVIGAYLGEKYAYAAR"
     gene            346..1050
                     /locus_tag="VM910_02120"
     CDS             346..1050
                     /locus_tag="VM910_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61382.1"
                     /translation="MLRVSNLSAFYDRTQVLHEINVRVEEREIVGLVGANGAGKTTTL
                     LSIMGLKTRIEGEIWLNEERIDHLPAYERVRRAIVLIPERRRLFPFMTILENLELGAY
                     LPGPRGDRNASLETVFDLLPVLAERRYQIAGSLSGGEQQMCAIGRALMSRPRLLMLDE
                     PTEGLAPLYVERLFDLIVRLRDDGITILLVEQNVEHVLKIADRGYVLENGRVVLQNSG
                     QGLLADPRLKTAYLGL"
     gene            1063..2076
                     /locus_tag="VM910_02125"
     CDS             1063..2076
                     /locus_tag="VM910_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI61383.1"
                     /translation="MITDIHIHHVPEPFARFIEKATPYLIRRDPPCGETVTLNVGPLR
                     YGLNRTFFDLDRLIKRMQEMSVDHAVLSLATPFVKFDVPASVGIETAQLYNDEIARIH
                     RAAPDTFDGWAFLPMQDPEAAANELRRAVRNLGLIGGYLPSNVKGRYLDSEEFAPIFE
                     AATQLDVPLMVHPSNPPARDRMGDYELAVVSGYLFDTTLNIFHMIFSGVFDRYAGLRL
                     CCTHLGGFAPLLRARMQRELDTNPGLAARLKRPLTAYLQSIYYDTICFEPVYLRAALE
                     SDAIDPAHLVLGSDTPFPLGEPDPVGFIERSFRGHCAEIADAILHRNASNLFTRCASG
                     VTR"
     gene            2073..2612
                     /locus_tag="VM910_02130"
     CDS             2073..2612
                     /locus_tag="VM910_02130"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HVI61384.1"
                     /translation="MNLRKLSLTKETIFADAGRLISQPITRAVAVAVIANPFADRFVD
                     DLSPLFADGAELGQRVMPDLSSLLSRPAVAYGKAAIVGVAGEIEHGAAMIHPRLGQPM
                     REAVGGGQAIISSNVKIGGPGTSIDVPLAHKDNIWSFDHLATVTISSDDAPRPDEIVV
                     IMAISDGGRPHPRVGKGRI"
     gene            complement(2661..2792)
                     /locus_tag="VM910_02135"
     CDS             complement(2661..2792)
                     /locus_tag="VM910_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61385.1"
                     /translation="MPDHATSKTINAALLKRFGAEFEIDPELTIRRKKWWNAPAWAP"
     gene            complement(3144..3299)
                     /locus_tag="VM910_02140"
     CDS             complement(3144..3299)
                     /locus_tag="VM910_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61386.1"
                     /translation="MSDVAARSSHSLPLPTPLVLCLFGLLGLLLAGLIVLIVAPQYLT
                     WVLSFIL"
     gene            complement(4058..4867)
                     /locus_tag="VM910_02145"
     CDS             complement(4058..4867)
                     /locus_tag="VM910_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HVI61387.1"
                     /translation="MRVRFGGAWIADSEHVVLLHEPGRYPVAYFPRDSIAAGVLEASD
                     YSSRHRELGPTSWYTVRAGGHSKPRAAWQHTELPSYASELQARVAFAWPAMDAFYEED
                     ERIVGHAADSYHRIDIRQTSRRLLVRCGERLVADSTRPLVLYESGFAPRWYVPRADIE
                     EALLTSVEGQTFCPYKGLASYYDIGDAHRAAWSYENAWTEVARICGLVSFEPDKVAVS
                     LDGARLTLESGQNVVAHGVDRDLAVEARVTAPVTAPPPRREMLRAGPGKPP"
     gene            complement(5020..5766)
                     /locus_tag="VM910_02150"
     CDS             complement(5020..5766)
                     /locus_tag="VM910_02150"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HVI61388.1"
                     /translation="MPHLQPRGRCIAPDLIGMGDSDKLPESGPGSYRFVEHRRYLDAL
                     LEALDVRERVTLVVHDWGSALGFDWANRHREAVKGIAFMEAIVRPQGWDHWDKINMRP
                     ALQALRSEAGEAMVLRDNFFIEKILPGAILRTLSTEEMAQYRRPFAEPGEGRRPTLTW
                     PRQIPIDGEPADVVAIVAASADWLSTSGVPKLFFRAEPGAILANDEDLAFIRRLPALT
                     EVKVAGRHYVQEDSPHEIGRAIASWMGALR"
     gene            complement(5930..>6286)
                     /locus_tag="VM910_02155"
     CDS             complement(5930..>6286)
                     /locus_tag="VM910_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI61389.1"
                     /translation="SLDNFYLARPGADEIQLDLFGDYKSNVALYPEFQHYFREHKPPF
                     LAVWGNNDPFFLPAGAQAFKRDMPHAHVRFFDTGHFALETHCDEIASAIKEFLVTPRP
                     STSRRVNGPLPQEKRT"
BASE COUNT         1193 a         1918 c         1927 g         1248 t
ORIGIN      
        1 ggatcggcga gcttgccgaa gcgtcagtgg caagtctcac cgtcggtcaa ctcaagaagc
       61 tggagcttgc ccgcgcgcta gcgaccgagc ctgtgttgct gttgctcgac gaagtcatgg
      121 ccggtctcac gcctccagaa atcaaggaga tgatgacggt gattgggtcg ttgccaaagc
      181 gcggcatcac tgtcgtttgg gtcgagcacg tgatgatggc gatcatgaac gtcgccgaac
      241 gcatcgtggt gatgcatcag ggtcagatta tcgcccaagg cacgcccgca gacgtttcca
      301 aaaatccggc cgttatcggc gcctacctcg gcgaaaaata tgcctatgct gcgcgttagc
      361 aatttgtctg ctttctatga ccgcacgcag gtcctgcacg agatcaacgt gcgagtcgaa
      421 gaacgggaga ttgtcgggct cgttggcgcc aacggcgcag gcaaaaccac gaccttgttg
      481 agcatcatgg gcctcaagac ccgcatcgaa ggcgaaatct ggttgaacga agagcgcatc
      541 gatcatttgc cggcgtacga gcgagttcgt cgggcgatcg tcctcattcc agaacgacgg
      601 cggctatttc cgtttatgac catcctcgaa aacctcgaac tgggggccta tctgccgggg
      661 ccgcgcgggg accgcaacgc cagcctagaa acggttttcg atctgctgcc cgttcttgcc
      721 gagcgccgct accagatagc gggttcgctc agcggcggag agcagcagat gtgcgccatc
      781 ggccgtgcac tgatgagtcg cccgcgactc ctcatgctcg atgaaccgac ggaaggcctc
      841 gcgccccttt atgtcgagcg cctgttcgat ctaatcgtgc gcctgcggga cgacggcatc
      901 accattcttc tcgtcgaaca gaatgttgag catgtcttga agatagccga tcgtggttat
      961 gtgctggaga atgggcgggt cgtgctgcaa aactccggac agggtctttt agcggacccg
     1021 cggttaaaaa ccgcctatct cggcctttga ggctgtcgag ccatgatcac cgacattcac
     1081 atccaccatg tgcccgaacc atttgcgcgg tttattgaaa aggccacgcc ctatctcatc
     1141 cgtcgcgacc cgccgtgcgg cgagacggtc accctcaatg tgggcccgtt gaggtatggg
     1201 ctcaatcgca cgttctttga ccttgatcgg ttgatcaagc gtatgcagga aatgagcgtg
     1261 gaccatgcag tcctttcgct cgctactcca tttgtaaagt ttgacgtccc tgcgagcgtc
     1321 gggatcgaga cagcccaact gtacaatgat gaaattgcaa gaatccacag agccgcgccg
     1381 gacacgttcg acggatgggc gttcttgccg atgcaggacc cagaggcggc ggccaatgaa
     1441 ttgcgccggg ccgtgcgcaa ccttggcttg atcggtgggt atctgccgtc gaacgtcaag
     1501 ggccgctatc tggattcgga agaattcgcg ccaatcttcg aagcggcgac ccaattggat
     1561 gttccgctta tggttcatcc gtccaatccg ccggcccgcg accggatggg cgattacgaa
     1621 ttggctgtcg tcagcgggta ccttttcgat accactctca atatctttca catgatcttc
     1681 agtggagtat tcgatcgata cgcgggtctg cggctctgct gcacgcatct cggcggcttt
     1741 gctcccttgt tgcgagcgcg tatgcaaaga gagctcgata ccaatcctgg gcttgccgcc
     1801 cgccttaaac gtccgctaac cgcctaccta caatcgatct actacgacac catttgtttt
     1861 gagccggtgt atttgcgtgc tgcgcttgag tccgatgcta tcgacccggc acacctcgtt
     1921 ctcggtagcg atacaccatt tccgctgggg gagccggatc ctgtcggctt tatcgaacgg
     1981 tcgtttcgcg gccactgcgc tgaaatagct gatgccatcc ttcatcggaa cgcgagcaac
     2041 ttgttcaccc gttgtgccag cggagtaacg cgatgaacct acgcaagctc agtttgacca
     2101 aggaaacaat cttcgcggac gcaggccggc tgatcagcca gcccattact cgggccgtcg
     2161 cggtggccgt cattgccaat ccgtttgctg atcgattcgt tgacgatctg tcgccgctat
     2221 ttgcagacgg ggccgagctt ggtcaacggg tcatgcctga cctttctagc ctcctttcgc
     2281 ggccggctgt cgcgtacggg aaagctgcaa ttgtcggcgt ggctggcgag atcgagcatg
     2341 gagcagcaat gatccacccg cgcctcggcc agccgatgcg ggaggcagtt ggcggcggtc
     2401 aggcgatcat ttcctcgaat gtcaaaattg gtggacctgg cacgtctatc gacgtcccat
     2461 tagcccacaa ggacaacatt tggtcgtttg accatctcgc tacggtgacg atttcgagcg
     2521 acgatgcgcc acgtcctgac gaaatcgtgg tgattatggc aatttcggat ggcggacgcc
     2581 cacaccctcg cgttggcaaa gggcgcattt gaatcatcag aaagcaaatg aggcaggtgc
     2641 gttacccatt aacagaagag ttagggtgcc cacgccggcg cattccacca tttcttccgg
     2701 cgaatggtca gttcggggtc aatctcgaat tccgcgccaa agcgcttgag cagcgccgcg
     2761 tttatcgtct tcgaagtcgc atgatccggc atgacccgcc tcgcgtgttg tagaccccgc
     2821 ccacgagcgg aaaatttgga ccgttattct tggagagctc cgctccaccg cggcatttcg
     2881 ataccgccaa tcgcagctgg gtcattttcg accgattgag ccggttttgc ctggcagatc
     2941 catcttcagg cttgcaaaaa tatcacagca attctttttc agatcttcac cggcgttcgc
     3001 acggaactct ccacagacgt tcgtagatca tgggcgggcg gttcccggtg tggcctagga
     3061 cgcaccgaga tcgcctggcc ccggaggagc aggtcccccg gggcctcccc ccgttggcgc
     3121 tgccgagtcg cggggaggca atatcaaagg atgaaggaaa gcacccaagt caggtactgc
     3181 ggcgcgacga tcagcacgat gaggcccgcg agcagcaggc ccaacaaacc gaataggcac
     3241 agcaccaacg gggtcggaag cggcagcgaa tggctcgagc gggcggcaac gtcggacatc
     3301 ggtgtttcct cgcgcatgcc gatgctgccc cggaggcgcc gtgtgaggga gttcacacgc
     3361 gccgggtctt cgagccgccg gcggcttagc cagggcgcgt gctcataacc tttccgtaag
     3421 cgcggcgagt accgcttctt tgcgaaagcg accgaagcgg gggctgcgct gccgcgaact
     3481 gtcgcgatgt gccattgcca cgtaaaccgg ccgaaatcct tgcggcgcat gggcggcgat
     3541 gggagagaaa gttgaatcgg tggaacattc gcctttacgc gggcgttact ttccgtcgcg
     3601 acgctttcag accaaaaggg aggtaacgat gaaacgcgct ttgatcgctg tcgcaaccgt
     3661 gggcattttg gcgccggccg cctgggtcac ggaggcgaac gcacaatatg gcgctccggc
     3721 tccagcctat agcggtgccc cagcctatgg cccttcgcaa acctatgccc cttctcgaag
     3781 atatgcccgt cgagcctacc gccgctctgc ccgtgccccg caaccgtctg gcggtaatct
     3841 cgtatacacc ccgggctctg gtccgccgcc ctatgacgtt tctcgtcaag catacgctca
     3901 acaaagctct ggcaagtggg aatggtacgg tgctcccggc cccaccaagc gcggcaacat
     3961 gtgcgttacc cacgtcgacc cgctgcgtgg ctacgggttc caggcggcgt gcaaaaagtg
     4021 agtgctcgtt cactcccggc gtgaacggaa acggagacta agggggtttc ccaggccccg
     4081 cccgcagcat ctcacgccgg gggggcggcg ctgtgactgg cgctgtgact cttgcctcaa
     4141 cggcgaggtc tcgatcgaca ccgtgcgcga ccacgttctg gcccgactcc agcgtgagcc
     4201 gtgcgccgtc aagactcacc gccaccttgt ccggctcgaa ggacacgaga ccgcagatgc
     4261 gggcgacctc ggtccaggca ttctcatagg accatgcagc gcggtgcgcg tcgccaatgt
     4321 cgtaatagct ggccagacct ttatacgggc agaaggtctg gccttcgacc gaggtcagca
     4381 aagcctcttc gatgtcggcg cgcggcacat accagcgcgg cgcgaagccg gactcgtaga
     4441 ggaccaacgg acgcgtgctg tcggcgacga gacgctcacc gcagcggacc agcaggcggc
     4501 ggctggtctg gcggatgtcg atgcggtggt agctatcggc ggcgtggcca acgatgcgtt
     4561 cgtcttcttc gtagaaagcg tccatggccg gccacgcgaa cgccaccctt gcttgcagct
     4621 cactggcgta ggagggcagt tcggtgtgtt gccacgccgc gcgcggtttg ctgtgcccgc
     4681 cggcgcggac ggtgtaccag gacgtcggcc cgagctcgcg gtggcgggag ctgtaatcgc
     4741 tagcttccag cacgccagcg gcaatgctgt cccgcgggaa gtaggcgacc gggtagcggc
     4801 caggctcgtg cagcagaacc acgtgctcgc tatcggcgat ccaggcgccg ccgaagcgca
     4861 cgcgcatgcg ccgccgcagg ggctcggcgt agaggagtct atctggcaac ggatctggaa
     4921 cgaggaagcg gccgactttt cctggtgaaa gcggcccctg ctgtcaggac aatcccattg
     4981 ttctttcctt tcgatgacag cggccctgca cggccgctgt cagcgcaatg cccccatcca
     5041 gcttgcgatg gcccgcccga tctcgtgcgg cgaatcttcc tggacgtaat ggcgccccgc
     5101 gaccttcacc tcggtcagcg ctggcaacct gcggatgaac gcgagatcct cgtcgttggc
     5161 caggatcgca ccgggctcgg ccctgaagaa cagcttgggc acgccgcttg ttgacagcca
     5221 gtccgcggag gcggccacga tcgcaaccac gtccgcaggc tcgccatcga tggggatttg
     5281 ccggggccac gtcagcgtcg gccgtcgccc ctcgccgggc tcggcgaacg gccgtcggta
     5341 ctgcgccatt tcctcggtgg agagggtgcg caagatggcc cccggcagga tcttctcgat
     5401 gaagaagttg tcgcgcagga ccatcgcctc gccggcctcg gaacgcagcg cctgcaaggc
     5461 tggacgcata tttatcttgt cccagtggtc ccagccctgc ggccgcacga tcgcctccat
     5521 gaacgcgatg cccttcaccg cctcgcggtg gcggttggcc cagtcgaagc cgagggccga
     5581 accccagtca tggacgacca aggtgacccg ctcgcgcaca tccagagcct cgagcagggc
     5641 gtcgaggtag cggcggtgct cgacgaaacg atacgagccg gggccactct cgggcagctt
     5701 gtcggagtcg cccatgccga tcaggtcggg ggcgatgcag cggcctcgtg gctgcaggtg
     5761 cggcaacacg ttgcgccaga ggtacgagtg ggattgccgt gcagcagcac gatggggtcg
     5821 ccctttccca cctccacgta cgccatctcg cggccaagga cccgccgccg ctgctttttg
     5881 tacggaaatg ccgcagagat cttgttcgct tcctttaatg ccgcagagat catgttcgct
     5941 tctcctgtgg tagcggacca ttcacccgcc tcgatgtgga gggtcttgga gtgaccagaa
     6001 actctttgat cgccgatgcg atttcgtcgc agtgcgtctc cagcgcgaag tggccggtat
     6061 cgaagaagcg cacatgcgcg tgcggcatgt cgcgtttgaa tgcctgcgca ccagctggca
     6121 ggaagaaggg atcgttgttg ccccaaaccg ccagaaacgg cggcttgtgt tcgcggaaat
     6181 agtgctggaa ttcgggatat agcgcgacgt tgcttttgta gtcgccgaag agatcgagct
     6241 gaatttcatc cgccccggga cgcgccaagt agaaattgtc gaggga
//
LOCUS       DATLJT010000097         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_923231,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000097 DATLJT010000000
VERSION     DATLJT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923231"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VM910_02160"
     CDS             complement(<1..570)
                     /locus_tag="VM910_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI61390.1"
                     /translation="MKFGIWTPLPHTIRPEPRMDHAVAEIKSCASLAGRKDGSFEFAC
                     DVVRRADELGFDTTLIAERFLGPDLSAWVLASALAMVTKRIELMVAVHPGMVTPQVVA
                     KMASSLDRISGGRCALNIVNGWWMEEFDLFSNGTWIGDAERYPRMGEYIQVIKGLWTD
                     SDFNFDGTYYRAHVRAALTGADGKVVIPDQ"
     gene            618..1682
                     /locus_tag="VM910_02165"
     CDS             618..1682
                     /locus_tag="VM910_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61391.1"
                     /translation="MYRSVLAILLAYVELWLQTRMEIAMLPVSQGLHLRGALALLLAL
                     PVPSALSAGAALAQTKIITGMVAHGPPQWPQYIATELGWFQQDNIELDFVTAGGSGAQ
                     QLAAGALDICHSGYPDFARASLQGAPVKIIINDIVASPYAVFAKPAIKQIADLKGKLI
                     SIGGNNDITFIYIKPFLASAGLKTSDVDFIFAKAAGDRFSALVAGGVDATILNHPAYS
                     KAVSLGFSNLGDTKPYAEDIPFTVWAANMPWAEKNRNALTAFARNYKRGVRWLYDAAN
                     KDRAIDILMKYARQARKDLEEAYDYQVTKLKLYGLDGDVSDKTYEKMAEGLAEIDFIK
                     KPFPPKSAIFDGSFVQQATN"
     gene            1682..>2080
                     /locus_tag="VM910_02170"
     CDS             1682..>2080
                     /locus_tag="VM910_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI61392.1"
                     /translation="MTSGGRLAQRVALVTGGGGEIGGAICRRFAAEGAEVAIADLDPA
                     KSEAVARAIAGSGGRALALPVDVADEASAKDAVERTIASFGRLTTLVNVAATVTPDGT
                     VETLSLDDWNKAIAVNLTGAFVMCKYAVPAL"
BASE COUNT          409 a          644 c          654 g          373 t
ORIGIN      
        1 ctgatcggga atgacgacct tgccgtcggc accggtcagc gccgcccgca catgcgcgcg
       61 atagtatgtg ccgtcaaaat tgaaatcgga atcggtccac aggcccttga tcacctggat
      121 gtactcgccc atgcgcggat agcgctcggc atcgccgatc caggtgccgt tgctgaagag
      181 gtcgaactcc tccatccacc aaccgttgac gatgttgagc gcacagcgtc caccgctgat
      241 gcggtcgagg ctggaagcca ttttcgcgac cacctgcggg gtcaccatac cgggatgcac
      301 ggcgaccatg agctcaatgc gcttggttac catggcaagc gcggaagcga gcacccaggc
      361 cgaaagatcg ggaccgagga agcgctcggc gatcagcgtt gtgtcgaagc cgagctcgtc
      421 ggcgcggcga actacgtcgc atgcgaactc gaaagacccg tccttgcggc cggcgaggct
      481 cgcgcatgat ttgatctccg cgaccgcgtg atccatgcgc ggttccggcc ggatggtgtg
      541 aggcagcgga gtccagattc cgaatttcat cggccgtctc ggcacgactg agcagtgcgg
      601 caatgagagc cagaaaaatg tataggtccg tattggcgat cttgctagcc tatgtcgagt
      661 tgtggctgca gacgcggatg gagattgcga tgttgcctgt ctcgcaaggc ctgcatttgc
      721 gcggtgcact ggccttgctg ttggccttgc ctgtaccgtc cgcgctgtcg gccggggccg
      781 cgctcgcgca aaccaagatc atcaccggga tggttgcgca cgggccgcca caatggccgc
      841 aatacatcgc gacggagctc ggctggttcc agcaggacaa tatagaactc gacttcgtga
      901 cggcgggcgg cagtggcgcc cagcaactcg ctgccggcgc gctcgatatc tgccatagcg
      961 gctaccccga tttcgcccgc gcgtcgctgc agggcgcgcc ggtgaagatc attatcaacg
     1021 acattgtggc gtcgccctac gcggtcttcg ccaagcctgc tatcaagcag atcgccgatc
     1081 tcaagggcaa gctgatcagc atcggaggca ataacgacat cacgttcatt tacatcaagc
     1141 cgttcctggc ctctgccgga ttgaaaacga gcgacgtcga tttcatcttc gccaaggcgg
     1201 cgggagaccg tttctccgcg ctggtcgcgg gtggagttga tgccacgatc ctcaaccacc
     1261 cggcctattc caaggcggtc tcgctcgggt tctcgaacct gggcgacacc aagccctatg
     1321 ccgaggacat tcctttcacc gtttgggccg cgaacatgcc ctgggccgag aagaaccgga
     1381 acgcgctcac ggcgttcgcg cgtaactaca agcgcggcgt gcgttggctc tacgatgccg
     1441 ccaataagga ccgggcaatc gacatcctga tgaaatacgc ccgacaagcc cgcaaggatc
     1501 ttgaagaagc ttacgactat caggtgacca agttgaagct ctatggtctc gacggcgacg
     1561 tatcggacaa gacctacgag aagatggccg agggactggc cgagatcgac tttatcaaga
     1621 agccattccc gcctaaatca gcgatcttcg acggcagttt cgtccagcag gcgaccaact
     1681 gatgacgagc gggggcaggc tggcacaacg ggtcgcactg gtcaccggcg gcggtggcga
     1741 gatcggcggc gcgatctgtc ggcgcttcgc cgcggaagga gccgaggtcg ccatcgccga
     1801 cctcgatccg gccaaatcgg aggcggtggc gcgcgccatc gccggcagcg gcggccgcgc
     1861 gctggcgctg ccggttgacg tcgccgacga ggcgagcgca aaggacgcgg tggagcgcac
     1921 catcgcgagc ttcgggagac tcaccacgct ggtcaatgtc gccgccaccg tcacgcccga
     1981 tggcacggtc gagacgcttt cgctcgatga ctggaacaag gccatcgcgg tcaatctcac
     2041 cggcgccttc gtgatgtgca agtatgccgt acccgcgttg
//
LOCUS       DATLJT010000098         5586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_923380,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000098 DATLJT010000000
VERSION     DATLJT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923380"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..743
                     /locus_tag="VM910_02175"
     CDS             249..743
                     /locus_tag="VM910_02175"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HVI61393.1"
                     /translation="MNFDQFLNWVEGTPIARAIRENEVLFPWIESLHVLAIALAVGTI
                     SIVDLRLIGLASRNRAVTILMREVLPYTWIAFGVAAITGLLLFSSNAPKYAHNFYFQA
                     KMALLVLAGCNMTAFHRIGTREIVRWDAAGGTPVAAKTVGGISLLVWIAVVVCGRWIG
                     FTLH"
     gene            827..1633
                     /locus_tag="VM910_02180"
     CDS             827..1633
                     /locus_tag="VM910_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61394.1"
                     /translation="MFVVLASTAAADAQNAPKKQPTIINPPPTAQDYADLAKLPDWSG
                     VWNPKITDQDQQARDNPPPWNEKVAKEVEKMWAEEKAGRPKLIFYGCFPEAHPSWMLV
                     THNAMEILFTPGRVTMLGESDGNRLRRIYTDGRPHPETPDPSFHGHSIGHWEGDTLVV
                     DTVDILPQAPLAISEAVGVPNDGDMHVTERIYLAEKDILHVELEITAPNILTKPWKTT
                     RIFFRQRARKFDIVEGVCQLGDYREAKDENGHHVFVPVEKTEWGNLVAPK"
     gene            1655..2086
                     /locus_tag="VM910_02185"
     CDS             1655..2086
                     /locus_tag="VM910_02185"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HVI61395.1"
                     /translation="MTNHFKLIGMLAVAATVAVGIASVSAHHSTTMFDHSITMTINGE
                     VVELRWVNPHVSLSVNGVVKERAEDGPAVWVMEMTSPGNLVRAGGWRRDAVKAGEKVE
                     VLFSPLRDAERKGGALKKLTVLATGEVLTANIREQERPGLE"
     gene            complement(2385..3515)
                     /locus_tag="VM910_02190"
     CDS             complement(2385..3515)
                     /locus_tag="VM910_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI61396.1"
                     /translation="MKGHIRERSPGHWAIILDTHDATGKRKRRWHSFAGTKREAQIQC
                     AKLVSEAQGGGAIDPSRVTLNEYLDRFLRDWVPLHLVARSVERYTGSLGHARRHLGDR
                     RLQSLRPADLAALYASLARSGLAPRTIRHVHRVLHRALGQAKLWGLLRDNPAETAKPP
                     TAPDQELAILQPDHARSVLAKLQGGPLYLLASLALATGMRRNEMLGLRWRDIDMDAGR
                     LTVEQALEQTAEHGIRIKGPKTRHGRRTISLPPHIVAELRSHWRAQQEQRLALGMGKA
                     PADSTVLATLDGKLQSPNAITKAWERAMDDIDMSEITLHSLRHTHASMLIASGMDILT
                     ISRRLGHANPTVTLGVYGHLIHGSDDKAAQIMEAAFGGGKAR"
     gene            3832..4035
                     /locus_tag="VM910_02195"
     CDS             3832..4035
                     /locus_tag="VM910_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61397.1"
                     /translation="MKDLMKELNENAVMRLWPEVGTLLKMQRSATYGAAKRGEIHTVK
                     FGHFKRVPTAWLKRKLGLEDAAA"
     gene            4150..4929
                     /locus_tag="VM910_02200"
     CDS             4150..4929
                     /locus_tag="VM910_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61398.1"
                     /translation="MPTGNNKKWPSRFCRKALRRREIERHALHVGAIDTEDRSRWLMA
                     WLWNNTPKNPKTYDPIWLLMDVARKRMGGSISEAEAAAIVEEAAATTPARMSDDSLAK
                     FLSVTYAQRQALGLRAIGSIDVGKRARKVLRRRKDRLYQEARRRARGARPHSESLSRT
                     KPWKAMKMSRAKWYRQRRETDSSASVLLYGDDRPVSAGMSARLPSSSFFSRSPLSSST
                     LASSRMATFIVANRRESNWHPKGLTPAVQRFLERRNAWVRQ"
     gene            complement(5215..5358)
                     /locus_tag="VM910_02205"
     CDS             complement(5215..5358)
                     /locus_tag="VM910_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61399.1"
                     /translation="MRIDRALKAAAEKAAQEEGRSLANLIIKLLRDHCEGLAKKQRNK
                     PSK"
     gene            complement(5348..>5586)
                     /locus_tag="VM910_02210"
     CDS             complement(5348..>5586)
                     /locus_tag="VM910_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61400.1"
                     /translation="TPPIIAMLPLEEEQDTNTNQEQANGVECCYFVAGDKPDQLLHDD
                     PPLIGSASLAADKIAFVSAAYYATYYRPEIRDAD"
BASE COUNT         1084 a         1673 c         1730 g         1099 t
ORIGIN      
        1 gctcgcgagc gtctcgatgc tgctctgggt gggcatcatt ttcgccggcc gttggatcgc
       61 ttattactac tagtgtccgg attccgaagt tcgcacgccc cttgcggcac catctgatgc
      121 gaacttcggt caaagggggc gcgcaaattt atgattctct ggcgcggttt tgcatttcct
      181 atgcgagatt gcgactacaa tggcaggaac ttcaaatccc ccacgctggc ggtgcaggac
      241 cgcatgtcat gaacttcgat cagttcctga attgggtaga aggtacgccc atcgcgcgtg
      301 cgatccgcga gaacgaggtc ctgtttccct ggatcgaatc gttgcatgtg ctcgcgattg
      361 cgctggcggt cgggaccatc tcgatcgtcg atttgcgcct gatcgggctt gcctcgcgca
      421 accgcgccgt gaccattctg atgcgcgaag tgctgcccta tacttggatc gccttcggcg
      481 tcgccgccat cactggcttg ctgctgtttt cctcgaacgc gccgaaatat gcgcacaatt
      541 tctatttcca ggccaagatg gcattgcttg tgcttgctgg ctgcaacatg acggcctttc
      601 atcggatcgg cacacgcgag atcgtgcgct gggacgcggc cggagggacg ccggtcgcgg
      661 ccaagactgt gggtggcatc tcgcttctgg tttggatcgc ggtggtggtg tgcggccgct
      721 ggatcggctt cacattgcac tagtccggcc gccggggtat ttttttgcga acggcccgcg
      781 tctcaatcgc gcgaagggcg accttatgcg attccgattg ctagcgatgt ttgttgtgct
      841 cgcctcgacc gcggcggccg acgcccagaa cgcaccgaag aagcagccga ccatcatcaa
      901 tccgccgccg accgcgcagg actatgccga tctcgccaag ttgccggact ggagtggcgt
      961 gtggaacccg aagatcaccg accaagacca gcaagcgcgc gacaatccgc cgccgtggaa
     1021 tgaaaaggtc gccaaggaag tcgagaagat gtgggccgag gagaaggccg ggcggccgaa
     1081 actgatcttc tacggctgct tccccgaggc gcatccgtcc tggatgctgg tcactcacaa
     1141 cgcgatggaa atcctgttta cccccggccg agtgacgatg ctcggggaat ccgacggcaa
     1201 ccggctgcgc cgcatctaca ccgacggtcg gccgcatccc gagacgccgg acccgtcttt
     1261 tcatggccac tcgatcggcc attgggaggg tgacacgctt gtcgtcgata ccgtcgacat
     1321 cctgccgcag gcgccgctcg cgatcagcga ggcggtcggc gtgccgaatg acggcgacat
     1381 gcacgtcacc gaacgcatct accttgccga gaaggacatc ctgcacgtgg agcttgagat
     1441 taccgcgccc aacatcctta ccaagccctg gaagaccacc cgcatcttct tccgccagcg
     1501 cgcgcgcaag ttcgacatcg tcgagggcgt atgccagttg ggcgactaca gggaagccaa
     1561 agatgagaac ggccatcacg tcttcgtccc ggtcgagaag accgagtggg gcaatctcgt
     1621 cgcgccaaaa taatccggca gttggatcga tctcatgacg aatcatttca agctcatcgg
     1681 catgctcgcc gttgccgcca cggttgcggt cggtatcgcg tcggtgtcgg cgcatcattc
     1741 caccaccatg ttcgatcatt ccatcaccat gacgatcaac ggcgaggtcg tggagctgcg
     1801 ctgggtcaat ccgcacgtca gcctctcggt caacggcgtc gtcaaggagc gggccgagga
     1861 cggcccagcg gtctgggtga tggagatgac gagccccggc aatctcgtgc gcgccggcgg
     1921 ttggcggcgc gacgcggtca aggccggcga gaaggtcgag gtcctgttca gcccgctgcg
     1981 tgacgccgag cgcaaggggg gagcgctgaa gaagctcacc gtgctcgcga ccggcgaagt
     2041 cttgaccgcc aacattcgcg agcaggagcg gccggggctc gaatgagctg gcgcttgggc
     2101 ttccgtccgg ccgcggcgtt ggccatcggc gtgttcatcg gcgcgccgtt tgctctcggc
     2161 caagactcga gtggcccgcc gccgaaggac acgatcttcg cccggaagat cttgatgggc
     2221 gcgatcggca cgaacatgga tgagatcgag accatgctgg cgccgaacgg caagttcgac
     2281 gccgcgaccc acgttgccgc agaaaacggg gctatttgcc ctggtctgct accattttgc
     2341 tacctaatgg gcgccgtcgg tgcgggacgg gctgtttcct ttccttatct ggccttcccg
     2401 cctccgaacg ccgcttccat aatctgcgca gccttatcgt cgctgccatg gatcaggtgg
     2461 ccatagacac caagcgtgac agtaggattg gcgtggccaa gacggcggct gatggtgagg
     2521 atgtccatgc cggacgcgat cagcatggag gcgtgggtat ggcgcaggct gtgaagcgtg
     2581 atttccgaca tgtcgatatc gtccatggcg cgttcccagg ccttggtaat cgcgttaggg
     2641 ctctgtagct tgccgtcgag ggtggcaagc accgtgctat cggccggggc cttccccatg
     2701 ccaagggcaa gccgctgctc ttgctgtgct cgccaatggc tgcggagttc ggccacgatg
     2761 tgagggggca gcgagatggt gcgccggccg tggcgcgtct tcggcccctt gatccgaatc
     2821 ccatgctcgg cggtctgctc cagcgcctgc tccaccgtca ggcggccggc atccatgtcg
     2881 atgtcccgcc accgcagccc cagcatttcg ttgcggcgca ttccggtcgc gagggccagg
     2941 gatgcaagca gatagagcgg cccaccctgg agcttggcca agacgctgcg ggcatgatcg
     3001 ggttgcagga ttgcaagctc ctggtccggc gccgtcggcg gcttggcggt ctcggcggga
     3061 ttgtcgcgca ggaggcccca gagcttggcc tgcccgagcg cccggtgcag gacccggtga
     3121 acatgcctga tcgtgcgggg cgccagcccg gaacgcgcta ggctcgcata gagggcggcg
     3181 aggtcggccg gccgcaagga ctgtaggcgt cggtcgccga gatggcggcg ggcgtgaccg
     3241 agcgacccgg tgtagcgctc caccgagcga gcgaccaggt gcagcggtac ccagtcgcgc
     3301 aggaaacggt ccagatactc gttgagcgtg acccgcgacg ggtcgatggc gcccccgccc
     3361 tgcgcctcgc tgacgagctt ggcacactgg atctgcgctt cgcgcttggt gccagcaaaa
     3421 ctatgccagc ggcgtttgcg cttgccggtc gcgtcgtgtg tgtcgaggat gatggcccaa
     3481 tgaccgggcg aacgttcgcg aatgtgcccc ttcattttgt tctcctcacg gtttgatcca
     3541 gccggtagac gatttcacta ttcaatgagc gccgattgcg agaggccaag cggacaagct
     3601 tgcggtgaag agcttccggt aggcgcaccc acaatttcgg gagtttccga ggtttccgag
     3661 gtttccgagt ttgtgccatt cgcgttacca acatcgctga gtgcggaatc acacggcttt
     3721 acatggcatc ggccgggttg acagtgaaaa ccagtcaagc gcatcctcta tcatatacca
     3781 cggaatcact tagtggcaca atgccactag agaaaaatag gagccaccgg ggtgaaagac
     3841 ctaatgaagg agctaaacga gaacgcggtt atgcggctat ggcccgaggt aggcacgctg
     3901 ctcaaaatgc agcgatccgc cacctatggt gccgccaaac gcggtgaaat ccacaccgtt
     3961 aaattcggac atttcaagcg tgtcccgaca gcttggctca aacgcaagct cggcctcgag
     4021 gacgccgccg cctaaacgaa acccggccgc ctggtgggca agcggccggg gtaagatagg
     4081 aatttctctc tctcacaagc gggaaattcc tatcgcgtct gcaatgcaaa tgcaagcgag
     4141 ggaatttcaa tgcccacagg aaataataag aaatggccga gccgattctg ccgtaaggcc
     4201 ctgcgccggc gtgaaatcga gcggcacgcc ctgcacgtcg gcgcaatcga taccgaggac
     4261 cgcagccggt ggcttatggc ctggttgtgg aacaacaccc ccaagaaccc caagacctac
     4321 gacccgatat ggctgctgat ggacgtggcc cgcaagcgga tgggcggctc gatcagcgag
     4381 gccgaagcgg ccgccattgt cgaggaagcc gccgcgacca cacctgctcg catgtctgac
     4441 gacagcttgg ccaaatttct aagcgtcacc tacgcgcaga gacaggccct cggtttaaga
     4501 gccatcggca gcatcgacgt ggggaagcgc gccagaaagg tgctgcgtcg ccgaaaggac
     4561 cgtctctatc aggaagcgcg ccgacgagcc cgcggtgccc gtccgcacag cgagtcactg
     4621 tcccgaacca agccgtggaa ggccatgaag atgagcaggg ccaagtggta cagacagcga
     4681 cgtgagacag attcgtcggc atccgtcttg ttatacggtg acgacagacc tgtctcagca
     4741 gggatgtcgg cacgccttcc ttcttcttct tttttttctc gttcccctct ttcttcttcg
     4801 acgctggcat cctcgcggat ggcgaccttc atcgttgcca acagacgtga gtcaaactgg
     4861 catcccaagg ggcttacacc cgctgtgcag aggttcctcg agcgccgtaa tgcatgggta
     4921 cgccaatgac gaaggggatg aattggcgtc gcgccaagct tgcctcgaag ccgagcctcg
     4981 atcatcgcta tgagttcacc gagtttaaac gagatcgcgc cgatagatgg ctcgcggcgg
     5041 tggaacgtcg ccagcgggag cagcgtcacc tatcggtgcg cgctggatcg tcctcgagcc
     5101 aataggagag aagtccaatg aaaacaatgg ggtcggtgac agtcaccgaa ccagttgaaa
     5161 acattagcaa aatcaaagct cgcgtcacgt gacgcaacgg ttgaaaacat tcaactattt
     5221 cgacggcttg ttgcgctgct ttttggccag cccttcgcaa tggtcccgca gcaacttgat
     5281 aatcagattg gccaagctgc ggccttcctc ctgcgcagcc ttctccgccg ctgccttcaa
     5341 tgcgcggtca atccgcatct cgaatctctg ggcggtagta agtggcatag taagcggcgc
     5401 tgacgaatgc gattttgtcg gctgctaacg atgccgagcc tattaatgga ggatcatcgt
     5461 gcaagagttg gtcaggtttg tctccagcaa caaagtagca acactcaacg ccgttggcct
     5521 gctcttgatt agtattggtg tcctgctcct cttccaacgg cagcatcgct atgattggtg
     5581 gagttg
//
LOCUS       DATLJT010000099         5329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1842089,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000099 DATLJT010000000
VERSION     DATLJT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842089"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VM910_02215"
     CDS             <1..357
                     /locus_tag="VM910_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61401.1"
                     /translation="RTAQYAYEAVLPDPASRLLTWVWPRWIPLDENARAYERFKWLEQ
                     ELSRSKLPALIVWGREDEVFDAATFSERFKQMLPHAEGPHLVTGRHFLQEDSGTEIAA
                     LIDSFLRRLDANEGKQ"
     gene            354..755
                     /locus_tag="VM910_02220"
     CDS             354..755
                     /locus_tag="VM910_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61402.1"
                     /translation="MTEVTLVATPSLEGKRLAFALAEDRVAHYPEFRDFFVRTFDLDR
                     KGLTEPGFVRTSSGNLYAFIFIGRSGAPFPSGLEIYGVVDALEPVDGAVLDRDLWSIL
                     RWVIGGVGAPWTVEDFDRTGRLYRLPAASAA"
     gene            1335..2054
                     /locus_tag="VM910_02225"
     CDS             1335..2054
                     /locus_tag="VM910_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI61403.1"
                     /translation="MIRFYYHPTPNPAKVALMLEELGLPYQVIPVDTGKGEQHKPEFR
                     AINPNGKVPAIVDTDGPDGKEARVFDSTAILLYLGHKTGRLIGSAADLPELLSWLLFI
                     ATGIGPFSGQAVHFQHAASEKLAYAINRYRREVERHYEVLDTHLKGREFIVGTDFSIA
                     DISAWGWLERAPRVLPGDAPPLAAFPEIDRWYRSIDARPAVTRARTVGKDHMFKKEVD
                     DETRRALFPSNYPKVARQSAA"
     gene            2085..2891
                     /locus_tag="VM910_02230"
     CDS             2085..2891
                     /locus_tag="VM910_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI61404.1"
                     /translation="MHLKGKKVLITGGSSGIGFAIAEATLAKGADVAITGRRQDVVME
                     AAKQLRQGGRRVESVAADVSTASGREATLKLSLEKLGGLDVLVNNAGGVRAGRLEDTT
                     EAEIRTMIEVNLVAPILLTRAALPALRASGDGLVVNVTSGIALVAAPFYATYAGVKGG
                     LSKFGESLRRELNGEGIRVMTVYPGATDTPMMRSSRAGPELGFTREPASAVAAAVIEG
                     IEQDAFEVIRGGEARAKMIALNREDPAALDKRFLDLKPALAEAVRDHSAL"
     gene            complement(3026..3763)
                     /locus_tag="VM910_02235"
     CDS             complement(3026..3763)
                     /locus_tag="VM910_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI61405.1"
                     /translation="MLETLALRQWRDYQWGTPGTAFADPEISLTLDEAYQVQMEVARL
                     RCTAGDAVAGYKVGCIGPSVVAQFGMSGPIHARLFSSEIRRSGESLRHIAYANPAIEG
                     EMALCVGADGQITAMFPVIELHQFVFRAPEKTLAELVANNGINVGVVIPAHLDMAPPG
                     DWLGARELSVAINGATIDSGALWALPGGAKEAVEWLRGDLARFGAALKPGDLVLAGTP
                     LGLHPVQPGDHVAVSVDGRECVDCRFI"
     gene            complement(3785..4030)
                     /locus_tag="VM910_02240"
     CDS             complement(3785..4030)
                     /locus_tag="VM910_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61406.1"
                     /translation="MTNNNGLVFRSLAATAVRTPIQIGENFTGLPPMAAALEAGASDF
                     VMPQELLQIMDGMAVIPGRPGNGLAWNEEAVARYRLA"
     gene            complement(4429..4617)
                     /locus_tag="VM910_02245"
     CDS             complement(4429..4617)
                     /locus_tag="VM910_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61407.1"
                     /translation="MTRLYIERDLEELAAKLLQSALKLPHGPERQKILEQISGFRVRI
                     NLLKAKTNLTPPQTVMNN"
     gene            complement(4789..5091)
                     /locus_tag="VM910_02250"
     CDS             complement(4789..5091)
                     /locus_tag="VM910_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI61408.1"
                     /translation="MFDMAKLKNLKKLDEGSSQAMKAFWAFDKAVFQEGAVSALNKQL
                     MAVAVALTTQCPYCIEIHTQEARKAGATDAQLAETAVVAAAIRAGGAITHATHLFG"
BASE COUNT         1033 a         1631 c         1575 g         1090 t
ORIGIN      
        1 cgcactgcgc agtacgccta tgaggctgta ttgccagacc cggccagccg ccttctcacc
       61 tgggtttggc cgcgctggat cccgttggat gagaacgcgc gcgcctatga gcgcttcaaa
      121 tggctcgagc aggagctgtc ccgatcgaag cttcctgcct tgatcgtgtg gggacgcgag
      181 gatgaggtct tcgatgctgc gacgttttct gagcgtttca agcaaatgct ccctcatgcc
      241 gaggggccgc acctcgtcac cggccgccat ttccttcaag aggattctgg gacggagatt
      301 gccgctctga tagactcctt tttgaggcgc ctggacgcga acgagggaaa acaatgactg
      361 aagttactct ggtggcaacg ccaagcctcg aaggaaagcg acttgccttc gctcttgccg
      421 aagatcgggt ggcgcactat cccgagttcc gcgacttctt cgtccgaacg tttgatctgg
      481 accgcaaggg acttaccgag ccgggattcg tccgcacctc atctggcaat ctttatgcct
      541 ttattttcat aggcaggagc ggggccccgt ttccatcggg cctcgagatc tatggcgtcg
      601 tggacgcgct cgaacctgtt gacggcgcag tcttggacag ggatctctgg tcaattctgc
      661 gttgggtaat cggaggcgtc ggggctccct ggaccgtgga agatttcgat cgcacagggc
      721 gcttgtatcg cctgccggcg gcgtcggccg cctaatctca catccgtccc caacgtccgc
      781 ttcagcccag ccgtctcgta acgcggcctg atgtgcaatc actgcatgcc gctcccggac
      841 gaaacagggg ttgatttgtt ttagagcaag tgatctaaaa gacggatcga tggtgacaat
      901 agccgacctt cagatgagcc tggcagcaga aagttcgaac gcagacctgg cttcgatttc
      961 gtccgattct tggggaatgg cgcgaggaat tgcggggtgg tctgtccgtt gcggtacttt
     1021 caggttggtt ctcgtgatgc gtctgggact ccgtcatgat cttcgggtat gggacgtttt
     1081 tgcgttgcgc tttggcgacg gcgcgctcct gctgacaccg gcgctgcttg tcgggccaat
     1141 acgcctacca ctcggcccgc gagccgcagc cgcaatcatt gcgcttgtgt cggtgacggc
     1201 gagcattttg gcgataccat ttctcgatga atggccatcc tggctgtccg ggacggccat
     1261 ctgcatcata gcaatggggg tggcaatggc tgctgcctct tccaaaccag cctaacatca
     1321 aagggagaat gctcatgatc cgcttctact accacccgac gcctaatccg gcaaaagtcg
     1381 cgctcatgct ggaggaacta ggtttgccct atcaggtgat tccggtcgac accggcaaag
     1441 gcgagcaaca caagccggaa tttcgcgcca tcaatcccaa cggcaaggtg cctgccatcg
     1501 tcgatacaga cgggcccgac ggaaaagaag cccgcgtctt cgattcgacc gcgattctgc
     1561 tctacctcgg acacaagact gggcgactga tcggctcagc ggcggacctg ccagaactcc
     1621 tgtcatggct cttgttcatc gcgacaggca tcggcccctt ctcaggacag gcggtgcact
     1681 ttcagcacgc cgcttcggaa aagctcgcct acgcgatcaa ccgctatcgt cgcgaagtcg
     1741 aacgccacta cgaagtgctc gacacgcatt tgaaaggaag ggaattcatc gtcgggacag
     1801 acttctcgat cgccgatatt tcggcgtggg gatggctgga gcgagcgcca cgcgtgttgc
     1861 cgggcgacgc gccgccgctt gccgcgtttc ccgagatcga ccgctggtac cgcagcatcg
     1921 atgcgcggcc tgccgtaacg agagcgcgca cggtcggcaa ggaccacatg tttaagaagg
     1981 aggtcgacga cgagacgcgg cgcgcgcttt tcccgtccaa ctatccgaag gtcgctcgcc
     2041 agagcgccgc ttgaatcctc caccttcaca agtaggcgaa caaaatgcac ctcaagggaa
     2101 agaaagttct gattaccggc ggttccagcg gcatcgggtt tgcgatcgcc gaggcgacgc
     2161 tggcgaaagg cgcagacgtt gccattaccg ggcgccggca ggatgttgtg atggaggccg
     2221 ccaagcagct tcgccagggc ggccgtcgtg tcgaatccgt ggcggccgac gtcagcactg
     2281 cgagcggccg cgaggccaca ctcaagctgt cgctggaaaa gctgggtggg cttgatgttc
     2341 tcgtcaacaa cgctggcggt gtcagggccg gtaggcttga ggatacgacc gaggccgaga
     2401 tccgaacgat gatcgaagtc aatctggttg cacccatctt gctcactcgg gccgctttgc
     2461 cagcgctccg cgccagcggc gatggccttg tcgtgaatgt cacgtcgggc atcgcgcttg
     2521 tcgccgcgcc gttctatgcg acctacgccg gcgtcaaagg cggtctgtcc aaattcggcg
     2581 aatccctccg ccgagagcta aacggcgagg ggattcgcgt tatgacagtc tatccggggg
     2641 cgaccgacac acccatgatg cgctcctcac gggcagggcc tgaactcggc ttcacccgtg
     2701 agccggccag cgcagttgct gcggcggtca tcgagggtat cgagcaagac gccttcgagg
     2761 tcattagggg tggcgaagcg cgcgcgaaaa tgatcgcttt aaatcgcgag gatccagcgg
     2821 cactcgacaa gcgcttcctc gacctcaagc ccgcgcttgc cgaagcggtt cgtgaccatt
     2881 ccgcgcttta ggctgaacac gatgtcccca gcagcaagcc gcgccgccgc tttggcggaa
     2941 atcaagcccc acccaccggc ccggtgggcg caggctcggc atggcccatg cgttgggatg
     3001 tcactcggtg tcccgtcggc cctctctaga tgaaccggca atcgacgcac tccctgccat
     3061 cgaccgacac cgccacgtgg tcgccgggct gcaccggatg caggccgagg ggagtgccgg
     3121 cgagcacaag gtctcctggc ttcaacgctg caccgaagcg agcaaggtcg ccccgcagcc
     3181 actcgacggc ctctttggct ccgccaggca gggcccatag tgctccgctg tcgatcgtcg
     3241 ccccattgat cgcaacagat agttcacgcg cgcccaacca atcgccaggc ggcgccatgt
     3301 cgagatgtgc tggtatgacc actccgacat tgatcccgtt attggcgacg agctccgcaa
     3361 gcgtcttttc tggcgcccgg aaaacgaatt gatgcagttc gatgacggga aacatcgctg
     3421 tgatttggcc gtcggcgccg acgcaaagcg ccatctcgcc ctcgatcgcg gggttggcat
     3481 aggcgatgtg ccgaagcgac tcaccggaac ggcggatttc gctgctgaat aggcgtgcat
     3541 ggatcggccc gctcatcccg aattgcgcca ccacgctcgg accgatgcag ccgaccttgt
     3601 atccagcaac cgcgtctccg gcggtgcaac gcagccgtgc aacttccatc tggacctgat
     3661 aggcttcatc gagcgtgagc gatatctcgg ggtcggcgaa ggcagttccc ggcgtacccc
     3721 actgatagtc gcgccactgc cgcagagcca atgtctccaa cattgtacga ggctgcggca
     3781 tcgcctaggc gaggcggtat cttgcgacgg cttcttcatt ccaagcgagc ccgttaccag
     3841 ggcgtcccgg aatgacggcc atgccgtcca tgatttgaag cagctcctgg ggcatgacaa
     3901 agtccgacgc gcctgcttca agcgccgccg ccatcggcgg cagtccggtg aagttctcac
     3961 caatctggat aggcgttctc acggcggtgg ctgccagcga acgaaacaca agcccgttgt
     4021 tattcgtcaa tctgctttct ccgcgccatt ccagtcgtat ctggagcttt ttcgatcgta
     4081 gcaattatag aacgttcgat cgacaatggc gcaagtaact gctaacgtca acgaaacgct
     4141 gcagcagggc tcgcgtttga gaccttacgg cgagcaaatc ggacaacgag tcggtatgat
     4201 gcgcttctgc gtcatcgagc tgcacttgct caagtgaaca gcttcgggag ttcgggccgc
     4261 tccgaggcaa tcatgcttgt cgccggcgcc acggtgatgg cagcacgcca aagcccgcgg
     4321 cggttgcgcc tgccgcacgc tcgtgcgttc caaagtcatc gcaacgaacg atgaaggcga
     4381 tgagcctatc ggctgatcag tctcgcggtc cgcgcgaggc tatcgccttc aattgttcat
     4441 caccgtttgc ggtggcgtta gattcgtctt tgcctttaat aggttgatcc gcacgcggaa
     4501 gcctgagatc tgctcgagga ttttctggcg ctccggtcca tgaggaagct tgagcgcaga
     4561 ctgtagcagc ttagcagcca gctcttccag atcccgttct atatagaggc gtgtcaaatt
     4621 cattctcctt tcacgttcgg ccggagccta caagacgaca gcagccggcg gcatggtcac
     4681 tttccaaact gcggtgcaac tgtcccgggc aacgagccgg acgctctgcc cacgccaact
     4741 tccgccgcgg gcaatccatc cacccggtgc cggccgccgc cagcatgatc agccaaacaa
     4801 atgtgtagcg tgtgtaatcg caccgcctgc gcgaatggcg gcagcaacaa cggccgtttc
     4861 ggcgagttgc gcgtcggttg ctccggcctt ccgggcttcc tgggtatgaa tttcgatgca
     4921 ataagggcat tgcgtggtga gcgcgaccgc aacggccatc agctgcttgt tcagcgcgga
     4981 gaccgctcct tcctgaaaca cggccttgtc gaacgcccaa aaggccttca tcgcctgcga
     5041 gcttccttcg tccagcttct tgaggttctt gagcttcgcc atatcgaaca taagatcttc
     5101 tcctctcaca tgtatgagtc atgcattatt tgtgcgtatt ggcaccaacg cacactgtgg
     5161 catcaccagg cgctggccag cggtgccgcg gtcgccaagg ttttagcgga catctagact
     5221 ctccgactag gtttgtgtcc ccgagtcgga cacgcacacc aattcgtcct gcaaaagaca
     5281 aatgttactg aacggctatc gtctcgaaga cgagttgagt cgcaccaaa
//
LOCUS       DATLJT010000100         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1842123,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000100 DATLJT010000000
VERSION     DATLJT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842123"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VM910_02255"
     CDS             complement(<1..618)
                     /locus_tag="VM910_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI61409.1"
                     /translation="MEDWSARQYLKFEDERTRPPRDLLAQVPLQKPRLAVDLGCGPGN
                     STELLVERFPHSEVIGLDSSPDMLRKARERLPNCKFVQDDIATWRPDPRTDLIFGNAV
                     MQWLPDHPAIMRRLLQAMPAGGVLAIQMPDNTREPALMFQREVGESGPWRDHPEIKAA
                     PREDLPSPEAYYDLLKPVCSAIDIWHIVYNHVMASPEAITEWFKGS"
     gene            691..1023
                     /locus_tag="VM910_02260"
     CDS             691..1023
                     /locus_tag="VM910_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61410.1"
                     /translation="MSLRQIIFAALVAALASSLAASFAGAALAADRSSQPIPCEDIDS
                     VGRAKMSADGTITLQLRSLWPDPIAESELVYPPDDPQYEEIKHHLGGIKPGESKPVPP
                     FCGLNSEP"
     gene            complement(1030..>2629)
                     /gene="ettA"
                     /locus_tag="VM910_02265"
     CDS             complement(1030..>2629)
                     /gene="ettA"
                     /locus_tag="VM910_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HVI61411.1"
                     /translation="NRKVLENINLSFYPDAKIGVLGVNGAGKSTLLRIMAGLDQDYAG
                     EAWAAEGARVGYLPQEPQLDREKNVRENVMQGVGPQKAILDRYNELATNYSDETADEM
                     TKLQDKIEAKGLWDLDSKVDQAMDALRCPPDDADVGKLSGGERRRVALCQLLLAQPDL
                     LLLDEPTNHLDAESVAWLEGHLRNYPGAILIVTHDRYFLDNVTGWILELDRGRSIPYE
                     GNYSAWLIQKQKRLEQEGREEEARQRTLAREQEWIAASPRARQAKSKARYQRYEELLQ
                     KAGEKQAQTAQIVIPVAERLGQNVIDFDHLRKGFADNLLIDELSFKLPAGGIVGIIGP
                     NGAGKTTLFRMITKQETPDQGTIQIGESVRLGYVDQSRDTLDGAKTVWEEISGGLDQL
                     QLGKREVNSRAYCAAFNFKGADQQKKVGQLSGGERNRVHLAKMLKSGANVLLLDEPTN
                     DLDVDTLRALEEALEDFAGCAVLISHDRWFLDRIATHILAFEGDSHVEWFEGNYQDYE
                     KDKMRRLGIESVIPQRIKYKKFSR"
BASE COUNT          461 a          797 c          809 g          562 t
ORIGIN      
        1 cgagcccttg aaccattcgg tgatggcttc tggcgatgcc atcacgtgat tgtagacgat
       61 gtgccagatg tcgatcgcgc tgcagaccgg tttgagcaga tcgtaatagg cttccggcga
      121 cggcagatcc tcgcgcggcg ctgctttgat ctccggatga tcccgccatg gcccactctc
      181 gccgacctcg cgctggaaca tgagcgccgg ttcgcgggtg ttatccggca tctgaatggc
      241 cagcacgccg cccgccggca tcgcctgcag caagcggcgc atgatcgccg gatgatcggg
      301 cagccattgc atgacggcgt tgccgaagat caggtcggta cgcgggtcgg gcctccaggt
      361 tgcaatgtcg tcctgcacga atttgcagtt cggcagccgt tcgcgcgcct tgcgcagcat
      421 atcgggcgag gaatcgagcc caatcacctc cgaatgtgga aagcgctcga ccaggagctc
      481 ggtggaattg cccggcccac agccgagatc gaccgcaagg cggggcttct gcagcggcac
      541 ttgcgcgagc aggtcgcgcg gcgggcgcgt gcgctcgtcc tcgaatttga gatattgtcg
      601 cgcgctccag tcttccatgc cgcttgcctg tgtttgagat tggcgatgcg agtagtttat
      661 tcctgtacct cctagcagga ggcgaccttg atgtctttgc ggcagataat ttttgcagcc
      721 cttgttgcag ccctggcgtc atcgctcgcc gccagctttg ccggagctgc gctggcagcc
      781 gaccgaagca gtcagccgat cccctgcgag gatattgaca gcgttgggcg ggcgaaaatg
      841 tccgccgatg gcacaatcac gctgcagctg cgttctctgt ggcccgatcc gattgctgaa
      901 agcgagctgg tctatcctcc cgacgatccg caatacgagg agatcaagca ccatctcggc
      961 ggcatcaagc ccggcgaatc caagccggtg ccgcccttct gtggattgaa ttccgagccg
     1021 taacaatcgc tagcgcgaaa atttcttgta tttgatccgc tgcggaatga cggattcgat
     1081 gccgaggcgg cgcattttgt ccttttcgta gtcttggtaa ttgccctcga accattcgac
     1141 atggctgtcg ccctcgaaag cgaggatatg ggtggcgatg cggtcgagaa accagcggtc
     1201 gtggctgatg agcacggcgc agccggcaaa gtcctccaac gcctcctcga gcgcgcgcag
     1261 cgtatcgacg tcgagatcgt tggtcggctc gtcgagcaag aggacattag caccggattt
     1321 gagcattttg gcgagatgca cgcggttgcg ctcgccgccg gaaagctggc cgaccttttt
     1381 ctgctggtcg gcacccttga agttgaaagc cgcgcaatag gcgcgcgaat tcacttcgcg
     1441 tttgccgagt tgcaactgat cgagccctcc cgaaatctct tcccagaccg tcttggcgcc
     1501 atcgagcgtg tcccgcgatt ggtcgacata gccgaggcgc accgactcac cgatctggat
     1561 cgtgccttga tccggcgtct cctgcttggt gatcatccga aacagtgtgg tcttgccggc
     1621 gccgttcggc ccgatgatac cgacaatgcc gccggctggc agcttgaagc tgagctcgtc
     1681 gatgagaaga ttgtcggcaa aacccttgcg gagatgatcg aagtcgatga cgttttggcc
     1741 gagccgctcg gccaccggaa tgacgatctg cgcggtctgc gcctgcttct cgcccgcttt
     1801 ctgcaacagc tcctcatagc gctggtagcg cgctttcgat ttcgcctgcc gcgcgcgcgg
     1861 cgaagcggcg atccattcct gctcgcgcgc cagcgtgcgc tggcgggcct cctcctcgcg
     1921 gccttcctgt tcgagccgtt tctgcttctg aatgagccaa gcggaatagt tgccctcgta
     1981 gggaatgctg cgaccgcgat cgagttcgag aatccagccg gtgacgttat cgaggaaata
     2041 gcggtcgtgg gtgacgatca aaatcgcgcc gggataattg cgcaaatggc cctcgagcca
     2101 ggctaccgac tcggcatcga gatggttggt cggctcgtcg aggagaagga gatcgggctg
     2161 cgccagcaga agctggcata gggccacccg ccgccgttcg ccgccggaga gcttccccac
     2221 atccgcatca tcgggtgggc agcgcagcgc gtccatcgcc tgatcgacct tggaatcgag
     2281 atcccaaagg cctttggctt cgatcttgtc ctgcaacttg gtcatttcgt cggcggtctc
     2341 gtcggaataa ttggtggcca gctcgttata gcggtcgagg atcgcttttt gcgggccgac
     2401 accctgcatg acgttttcgc gtacgttttt ttcacgatcg agctgcggct cctggggcag
     2461 atagccgacg cgcgcgccct cggcggccca ggcttcgcct gcatagtcct ggtcgagccc
     2521 cgccataatg cgtaagagcg tcgacttgcc ggcgccgttg actcccagca cgccgatctt
     2581 ggcgtccgga tagaacgaca gattgatgtt ctccaagacc ttgcggttg
//
LOCUS       DATLJT010000101         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3910145,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000101 DATLJT010000000
VERSION     DATLJT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910145"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..544
                     /locus_tag="VM910_02270"
     CDS             5..544
                     /locus_tag="VM910_02270"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVI61412.1"
                     /translation="MTFDLDLKSSIRTIVDYPKPGIMFRDITTLLADARAFRRAVDEL
                     VHPWAGSKIDKVAGIEARGFILGGAIAHQVSAGFVPIRKKGKLPHTTVRIAYSLEYGI
                     DEMEMHVDAIHPGERVILVDDLIATGGTAEGAVKLLRQIGANVVAACFIIDLPDLGGA
                     AKLRAMEVPVRTLMTFEGH"
     gene            complement(563..973)
                     /locus_tag="VM910_02275"
     CDS             complement(563..973)
                     /locus_tag="VM910_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI61413.1"
                     /translation="MREVNIGSASDFADPGRKIIGFEGFEVGVFKLDGEFFAYLNLCP
                     HMGGPVCQGKMIAKVEEIIAEDRTSKGMAFSKTRMHVVCPWHSYEFDIRTGVHPGNAR
                     ARLRKIKVTVSDGEVIIGVPDARERVPALPEMGE"
     gene            complement(987..1286)
                     /locus_tag="VM910_02280"
     CDS             complement(987..1286)
                     /locus_tag="VM910_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61414.1"
                     /translation="MAITTGTELRSLGADFVEAAERAMSSGRPEQVSDAELERVMTAA
                     VRLYAAKAEAAESPAPPVAPESVTPTDIVVTVSALIRAAGLNLWDVSMWFNRAKR"
     gene            complement(1286..2539)
                     /locus_tag="VM910_02285"
     CDS             complement(1286..2539)
                     /locus_tag="VM910_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI61415.1"
                     /translation="MLERVPQQFRELQEITMQTDTRDILAHATRQAESHEDYFLVDID
                     AHVTETSFWPEILALIDNDVIRQMGEANAARPGNANHSLLNHQPGILHQHLYGRIPHQ
                     QGLLEPIEKKGTHRFTELARRAMDALGLDYQIVFPTPMLSLGMHPQDEMEVALGGAYN
                     KWLVERILPEDDRLKGLLYLPFNTPEACIEQVERFADVDSVIGFTVCSTRNKPVHHNS
                     YMKLYALMEETGKPFAFHSGFNWNDPSFLQLNRFISMHALSFVHYNLIHMTNWIINGL
                     PERFPKLKVVWVESGLAWIPFLMQRLDHEYQMRSCEAPLLKKLPSEYMRDMFYTSQPL
                     EKTNPKLLQATLEAFNADTQLLYASDWPHWDFDPPSAITTLPFLTEQAKRNILGLNAA
                     RVFNLEVKRKRPKARDVLAARPGIS"
     gene            complement(2615..>3367)
                     /locus_tag="VM910_02290"
     CDS             complement(2615..>3367)
                     /locus_tag="VM910_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI61416.1"
                     /translation="DLAEDEPVRYTIIDRAPRYHLRDAPWGTQYAGYRPAGPDTTYWR
                     FANFLFPFWTQAPNGEFSSHMHARGWVPLDDAHTMFVFIWWKKAVSAMSLPQPAFKDG
                     TPIGGTGRGNKFLPNTSDWLGRFRLEANPDNDWRIDRAAQQSGAIYSGIDGIHLQDQA
                     ITESMGPIVDHAWEHLAPSDQMITRTRRRLLMAARALRDQGIAPPGAEDASVYRAARS
                     GYFVSDDKSEWQQVYANQVAAGVHPPRLHAAE"
BASE COUNT          586 a         1037 c         1066 g          678 t
ORIGIN      
        1 gcctatgact ttcgatcttg atttgaagtc gagcatccgg accatcgtgg attatccgaa
       61 gccgggaatc atgttccgcg acatcaccac gctgctggcc gatgcgcggg ccttccgccg
      121 cgcggtcgac gagctggtgc atccctgggc cggctcgaag atcgacaagg ttgccggcat
      181 cgaagcgcgc ggtttcatcc tgggcggggc gatcgcccat caggtctccg ccggcttcgt
      241 gccgatccgg aaaaaaggca agctgccgca taccacggtg cggatcgctt attcgctgga
      301 atatggcatc gacgagatgg agatgcatgt cgatgccatc caccctggcg agcgcgtgat
      361 cctggtcgac gatctcatcg cgaccggcgg caccgcggag ggcgcggtga aactgttgcg
      421 gcagatcggc gccaacgtgg ttgccgcctg tttcatcatc gacctgccgg atctgggggg
      481 cgccgccaag ctgcgcgcca tggaggtgcc cgtgcgcacg ctgatgacgt ttgaggggca
      541 ctgagcttct gaaaagatcg ccttattcac ccatctcggg cagcgccggc acgcgttcgc
      601 gcgcatcggg aacgccgatg atcacctcgc cgtcggagac ggtgaccttg atcttgcgca
      661 ggcgtgcccg cgcattgccg ggatgcacgc cggtacggat atcgaactcg tagctgtgcc
      721 aggggcagac cacgtgcatc ctcgttttgg agaacgccat gcccttgctg gtgcgatctt
      781 cggcgatgat ctcttcgacc ttggcgatca tcttgccctg gcacaccggt ccgcccatgt
      841 gcggacacag gttgagatag gcgaagaact cgccatcgag cttgaagacc ccgacttcaa
      901 agccctcgaa gccgatgatt ttacgtccgg ggtcggcaaa gtccgacgcg gatccaatgt
      961 tcacctcacg cattttggcg tctccgctat cgcttcgccc ggttgaacca catcgacacg
     1021 tcccagagat tgaggccggc cgcgcggatc agtgcgctca ccgttacgac gatatcggtc
     1081 ggcgtgacgc tctccggggc caccggcggc gccggtgatt ccgcggcttc ggcctttgcc
     1141 gcatagagcc gcacggctgc cgtcatcacc cgctcgagct cggcgtccga cacctgctcc
     1201 ggccggcctg aggacatcgc gcgttcggca gcctcgacaa agtcggcgcc gagcgaacgc
     1261 aattcggtcc cggtggtgat cgccatcagg aaatccccgg ccgcgccgcc agcacgtccc
     1321 tggctttcgg ccgcttgcgc tttacctcga gattgaacac acgggcggcg ttgagcccga
     1381 ggatgttgcg cttggcctgt tcggtgagga acggcaacgt ggtgatcgcg ctcggcggat
     1441 cgaaatccca atgcggccag tcggacgcgt aaagcaactg agtgtcggcg ttgaaggctt
     1501 cgagtgtcgc ctgcaacagc tttggattgg tcttttccag cggctggctg gtatagaaca
     1561 tgtcgcgcat gtattcgctc ggcaatttct tcaggagcgg cgcctcgcac gaacgcatct
     1621 gatattcgtg gtcgagccgc tgcatcagaa acggaatcca ggcgagcccg ctttccaccc
     1681 acaccacctt cagcttcggg aagcgctcag gcagcccgtt gatgatccag ttggtcatgt
     1741 ggatcaggtt gtaatgcacg aaggacagcg cgtgcatgga gatgaagcga ttgagctgca
     1801 ggaatgacgg gtcgttccaa ttgaagccgg aatggaatgc gaacggcttt cccgtctcct
     1861 ccatcagcgc gtacagcttc atgtagctgt tgtggtgcac cggcttgttg cgggtcgaac
     1921 agacggtaaa gccgatcacg ctgtccacat ccgcaaaacg ctcgacctgt tcgatgcagg
     1981 cttccggcgt gttgaacggc aggtacaaca gtcctttcag ccggtcgtcc tcgggcagga
     2041 ttctttccac cagccatttg ttgtaggcgc cgccgagtgc aacctccatt tcgtcctgcg
     2101 ggtgcatgcc gagcgagagc atcggcgtcg ggaagacgat ctggtagtcg aggccgagcg
     2161 cgtccatggc gcggcgcgcg agctcggtga agcggtgagt gcctttcttt tcgatcggct
     2221 cgagcagccc ctgctgatgc ggaatgcggc cataaagatg ctggtgcagg atgccgggct
     2281 ggtggttgag cagcgaatga ttggcgttgc cgggccgcgc cgcattggct tcgcccattt
     2341 gccggatcac gtcgttgtcg atcaacgcga ggatttccgg ccagaacgac gtctccgtca
     2401 catgcgcgtc gatatcgacc aggaaatagt cttcgtggct ttcggcctgc cgcgtggcat
     2461 gcgcgaggat atcgcgcgtg tccgtctgca tggtgatttc ctgcagttcg cggaactgtt
     2521 gcggaactcg ttcgagcatt gcggacctcc acggtcagcc gcagacgatg cggcgatcaa
     2581 tccggactca aatcacacgt ttcactttca catctcactc cgcggcgtgc agtcgcggcg
     2641 gatgcacgcc cgcggcaacc tgattggcat agacctgttg ccactcgctc ttgtcgtcgc
     2701 tgacgaaata gccgctgcgt gcggcgcgat agacacttgc atcttccgcg ccgggcggcg
     2761 cgatgccctg gtcgcgcagc gcgcgcgccg ccatcagaag ccggcgcctg gtgcgcgtga
     2821 tcatctgatc cgaaggcgca agatgctccc acgcatgatc gacgatcggc cccatgcttt
     2881 ctgtgatcgc ctggtcctgc aaatgaatgc cgtcgatccc gctgtagatc gcgccgctct
     2941 gctgggccgc gcgatcgatt ctccagtcgt tgtccgggtt cgcctcgagc cggaagcgcc
     3001 cgagccagtc cgacgtgttg ggcaggaact tgttgccgcg gccggtgcct ccgatcggcg
     3061 tgccgtcctt gaaggccggc tgcggcagcg acatggccga caccgccttc ttccaccaga
     3121 tgaatacgaa catggtgtgg gcgtcgtcga gcggcaccca gccgcgtgcg tgcatgtggc
     3181 tcgaaaactc gccgttgggc gcctgcgtcc agaacggaaa caggaagttg gcgaagcgcc
     3241 aataggtggt gtcggggcca gccgggcgat agccggcata ttgcgttccc cagggcgcat
     3301 cgcgcagatg atacctcggc gcgcgatcga tgatggtata gcgcaccggt tcgtcctcgg
     3361 cgaggtc
//
LOCUS       DATLJT010000102         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1842424,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000102 DATLJT010000000
VERSION     DATLJT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842424"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..553)
                     /locus_tag="VM910_02295"
     CDS             complement(266..553)
                     /locus_tag="VM910_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61417.1"
                     /translation="MSGLIDLFPFQNRARAPLFQPGGFKKMAVIAFTDAQLEQIMTTA
                     AQIPRRLRDAYLQRVVQLLNGRDFGDGDVHRAARAAAGEIMHGTRPVQASR"
     gene            924..1208
                     /locus_tag="VM910_02300"
     CDS             924..1208
                     /locus_tag="VM910_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61418.1"
                     /translation="MAVCLLPGTELAFDKPVQYQTALVFANTGHSVAIFRQINKGALH
                     TNHDALEFPNGKIVLLTRLYEGQHATVLTLPMQPKSAIEAEAQQRVAYVG"
     gene            1341..1589
                     /locus_tag="VM910_02305"
     CDS             1341..1589
                     /locus_tag="VM910_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HVI61419.1"
                     /translation="MDIPHELRDEFPQEISLIERMIRTNYEFRRLAERYDEVNRNIYR
                     IESGEEPTTDEVLARLKKWRLKLKDEIAAILTKLEHRM"
BASE COUNT          435 a          613 c          549 g          410 t
ORIGIN      
        1 gtggcgccgc cacgtatttg ttcccttgcc catcgcatgt agacttgagc ggtccattga
       61 cttagatcga aatgcttctt taaccacgag ctttccgaag tcgcgcgggc cttcggcaga
      121 cggcctgccg ctcctgcacg aactcatcgc gcaaaggggg tggctcaaaa gtggccaaaa
      181 gtggccacag aagccaaacg acagttgctt gcgattgctt ccaggtatga acggcttgca
      241 gatcacatca agcaacggcc gaaatctacc tactagcctg caccggacgc gtcccgtgca
      301 tgatctcgcc cgcagcagca cgggcggccc gatgcacatc gccatccccg aaatcccggc
      361 cgttcaatag ctggacgacc cgttgcaaat acgcgtcacg aaggcggcgc gggatctgcg
      421 ctgcggtcgt catgatctgc tcgagctgcg cgtcggtgaa cgcgatcacc gccatctttt
      481 tgaatcctcc cggctggaaa agtggggctc gcgcccggtt ttggaagggg aagaggtcga
      541 tcagacccga caagccttgc tcgaaatcta ttgcgcggtc gtcctggcga cgccttacaa
      601 cgatttcgca acgcactgag cgacgtccga ctacccggcc ttgaggtaga cctactcgcg
      661 gcgccagctc ttggcgaggt ggccatcgcg gcctcgcgcg ttgccatagc cgtgggttgg
      721 ccgcgcccgt tcgtgactga tgcaccgcgg cgtcttttct gttaatgttc cattatcggg
      781 ggcttcgaag aaaggagttc cttgatatgt gcgactatag tctgcacagt gtgcgaacac
      841 gcccggccaa agtgggtgaa aaactcgtta catacaactt tggcaccggc acacgaggat
      901 tcgccgcgcc agaggatact tcggtggctg tctgcttact tcctggtacc gaactcgcct
      961 tcgacaagcc agtccagtat caaaccgccc tcgtgtttgc caataccggc cacagcgtcg
     1021 cgatctttcg ccagattaac aagggcgcgc ttcacaccaa tcatgacgcg ctcgagttcc
     1081 cgaatggcaa gatcgtgctg ctgacgcggc tctatgaagg tcagcacgca actgtcctca
     1141 cgcttcccat gcaacccaag agcgctatcg aagcggaagc ccaacagcgc gtcgcctacg
     1201 tcggctgaca ttcatcgtcg tgaaaaaccg cgaacaagag ttcaggccgc tccctctcgg
     1261 gcggcctcct tcccgccgtc taaattcggg gcctttctca cactgctacg gacgcgcgga
     1321 atctcaagga atgcctagcc atggatattc cacacgaatt acgagatgag tttccgcaag
     1381 agatctcgct tatcgagcgg atgataagaa ccaattatga gttcaggcga ctcgcggagc
     1441 gatacgacga agtcaatcga aatatctatc gcatcgagtc gggggaagag ccgaccaccg
     1501 acgaggttct cgcacggctc aagaagtggc gtctgaagct caaggatgag attgcagcga
     1561 tcctcacgaa gttggagcat cgaatgtagg atctccgcgg cctcctttac tctcgccaaa
     1621 cgctttgccg ctacggctgc ttcctatgtg aattcaagcg tgtccgcttt ggcattggtc
     1681 ggggccccat atgtcttgtc ccaccgacga catctggaaa ttggtgatcg tgtacaaggt
     1741 gaggttccag ctgcccctgc gatcttgagc cacggccgca gagccgacca gtccgagcac
     1801 catcacaatg agcaacagac tgcgcattca caaaaacctg ttgagatcgc accttggacg
     1861 cctggcgcgg cgcaaacgcg catctcattc gcgcaacagc cgctcaatat tctgtgcctt
     1921 gtcgaagttc ttggcggaac cgagcccaat cggcgcaaca agcctcgccg atgcgagctc
     1981 ggctgcgagg aaatccgcgc cggcaac
//
LOCUS       DATLJT010000103         1813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2072502,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000103 DATLJT010000000
VERSION     DATLJT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072502"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM910_02310"
     CDS             complement(<1..692)
                     /locus_tag="VM910_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61420.1"
                     /translation="MRAPSPARAIALFVACAASSPASAGELAFQRLAELVSVDVSANL
                     QALQDDFAQAPAEARRLGAAIATALGDGTAIRAVTFVLILVIAGAGLEWLYWTFAAAP
                     LRGIVSASAATPREAALLALRRLGFLGFGLVLFTASTVGATLILPWPANIDVMVIAAA
                     ALVVAVRCAWIMADVVVSPHHPSQRLAPIEQYKSGLVVGGVAWLSILAATAILLPQLL
                     TTLAAAPHLAGT"
     gene            complement(689..>1813)
                     /locus_tag="VM910_02315"
     CDS             complement(689..>1813)
                     /locus_tag="VM910_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI61421.1"
                     /translation="MLFSRLIARRVRLISGLVLLLFVAGHLSNLALGLASVDAMERWR
                     GVLLRPWQTGFGQALLLTAAIIHAGLGLASLASRRSLAMSRTDWVQLALGLATPPLLV
                     NHVVGGQVTSDLMARFSADYGFVLALYWRYAPILALQQLLVVVIVWVHGAIGLYSTLV
                     LKRSWPRLAPIVVPILFAIPILALLGFAHAGEAVLARLETDTAWWALIEQNLRILQEM
                     GHRLDLIEHGVFLVYGIAVALAVGILVVNILRRRRTRVTVSYDGGVTAVGRVGMSVLE
                     VSRANDIPHACVCGGRGRCGTCRIIVLADSDLDSPAEAELATLARVKAPADARLACQA
                     HLLGRPVSVRRVYPAFVDAEAAREPGSWSVATELDLETVP"
BASE COUNT          400 a          630 c          544 g          239 t
ORIGIN      
        1 attgttccgg ccagatgcgg cgccgcggcg agggtcgtca gcaactgcgg gagaaggatc
       61 gccgtggcgg ccaaaatcga gagccaagcc acgccaccaa caacaagccc ggatttgtac
      121 tgttcaatcg gggcgagccg ctgcgacggg tgatgcggtg aaacgacaac gtcagccata
      181 atccaggcgc agcgtaccgc gaccaccagc gctgccgccg cgatcaccat cacatcgata
      241 ttcgcaggcc acggcaggat caacgtcgca cccaccgtgg acgcggtgaa caacaccaag
      301 ccaaaaccga gaaaccccag ccgccgcaat gccagaagag ccgcctcgcg tggcgttgcc
      361 gccgacgcgc tgacaatgcc tcgcagcggt gcggcggcaa atgtccagta caaccattcg
      421 aggccggcgc cggcgattac gaggatcaag acgaaagtga ctgcccggat cgcggttccg
      481 tcacccagtg ccgtggcgat ggccgcacca agtcgtcggg cttctgccgg cgcctgtgca
      541 aaatcgtcct ggagagcctg cagattggcg ctcacatcga cggacaccag ctcggccagt
      601 ctttgaaacg cgagttcgcc tgcgctcgcc ggcgacgagg ccgcgcacgc gacaaataag
      661 gcgatcgccc tcgcgggaga cggcgctctc acggcacggt ttccagatct agttcggtcg
      721 ccaccgacca gctcccgggc tcccttgccg cctccgcgtc aacaaaggcg ggataaaccc
      781 gtcgtacact caccggcctc cccaacagat gggcctgaca ggcaagccgc gcgtcggccg
      841 gcgccttcac ccgagcgagg gttgccagct ctgcttcagc cggggaatcg agatcgctat
      901 cggccagaac gatgatccgg cacgtcccgc aacggccgcg gcccccgcag acgcacgcat
      961 gcggaatgtc gttcgcccgg ctgacctcga gcaccgacat cccgacacgg ccaaccgcgg
     1021 tcacgccgcc atcgtaggac accgtgacgc gtgtgcgccg ccgtctcaga atgttaacga
     1081 cgagtatccc gaccgccaag gccaccgcga tgccgtagac caggaagacc ccatgctcga
     1141 tcaaatccag gcgatgaccc atttcttgta ggatccgcag gttctgctcg atcagggccc
     1201 accaagcagt gtcggtctcg aggcgcgcca acacggcctc gccggcatgc gcaaatccca
     1261 aaagcgcaag aatcggaatc gcgaagagta tcgggacgac gatcggggcc agtcgcggcc
     1321 aggagcgctt caacaccagc gtcgaataga gaccgatcgc gccgtgaacc cagacgatca
     1381 ccaccaccaa aagctgctgc agtgcgagga tcggcgcata tcgccagtaa agggcgagga
     1441 cgaacccata gtcggcgctg aaccgggcca tcagatcgct cgtcacctga cccccgacga
     1501 catgattgac gagcagaggc ggtgtcgcga gacccagcgc cagctggacc caatccgtcc
     1561 tgctcatcgc cagcgaacgg cgcgacgcca gcgaggcaag ccccagcccg gcgtgaataa
     1621 tcgctgccgt cagcagcagc gcctggccga agccggtctg ccacggcctc aagagtacgc
     1681 cccgccaccg ctccatcgcg tccaccgacg ccaagcccaa ggcgaggttg ctgagatgcc
     1741 cggcaacgaa gagaagcagg accaggccgc tgatcaggcg gacgcgtcgt gcgatcaacc
     1801 gagaaaacag cat
//
LOCUS       DATLJT010000104         4504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1153571,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000104 DATLJT010000000
VERSION     DATLJT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153571"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1870
                     /locus_tag="VM910_02320"
     CDS             <1..1870
                     /locus_tag="VM910_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI61422.1"
                     /translation="AFKGSSEIGFTIVSISVSLVAVLIPLLLMGGIIGRLFREFAVVL
                     AMTIFVSMVVSLTLTPMMASRFLRAHSETRHGRFYQWSERCFDAMLHAYERGLDVALS
                     WKRTTLIVFFATLALSVYLFVIIPKGFFPQQDNGLITATSEASQDISFEAMKAKQEEL
                     SRIVMQDPAVATVAMAIGGSGRAGNNGNLFITLKPRRERDASAQQVISRLRPKLEKVE
                     GARLYMQAAQDVRLGGRPTRTQFEFTLQDANLAELNEWAPKILARMQTLPELRDVATD
                     QQTQGTTLELKINRDTASRYGIYPQLIDDTLYDAFGQRQVTQYFTQLNSYHVILEILP
                     ELQGRIDSLDKLYIRSPTTGDQVPISTFATWTSVPVRPLSISHQGQFPAITISFNLAQ
                     GVALGQATDAVQKAMTELGAPATLNSSFQGTAQAFQQSLGTVPLLILAALVVVYLILG
                     ILYESYIHPITILSTLPSAGVGALAILMAFGYDFSLIALIGIILLIGIVKKNGIMMVD
                     FAIAAERDEHLSPEQSIRQAALLRFRPIMMTTMAALLGGVPLMLGTGTGSEIRQPLGY
                     AMVGGLIVSQALTLFTTPVIYLYLDKLSNAFSNWGRSGGARDDREPESASVKQAAE"
     gene            1933..2514
                     /locus_tag="VM910_02325"
     CDS             1933..2514
                     /locus_tag="VM910_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI61423.1"
                     /translation="MIVKLAAVLGVVAFGWTPGLAEQAPKKTPPHTAQPSTQPVVPTP
                     PQSDAPATSPPTPPAPNWASRCTSPSRDAPLECAIEETAVLTKTGQLIVLVNIRVAGD
                     ARSPVALVQLPLGLNLPGGARLQVDDGKVVELLIQTCENRGCYANTQISPDLLAALKS
                     GKQLKVSFQNLAKETITIPMPLADFTAAYDKIK"
     gene            complement(2611..>4504)
                     /locus_tag="VM910_02330"
     CDS             complement(2611..>4504)
                     /locus_tag="VM910_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVI61424.1"
                     /translation="RNYMVGKWHVTPLTESGATGPFDGWPLGRGFDRFYGFLDAETDQ
                     YAPELVSDNTHIDPPGTHAGGYHLTSDLVDHAIRFIGDHVADRPDLPWLTWVAFGACH
                     APHQAPADIIRGYDALFEHGWDVEREQRLARQKAMGLVPLDTSLPPRNDGVKPWDEHS
                     ADEKRVFTRLQSAFAGMLDHADRHLARLISFLEAAGIRENTLVIVMSDNGASQEGGPL
                     GFINAMGPYNFRPESIPEKLRRLDDIGGPGTHSNFPHGWAMASNTPLRRYKQNTHGGG
                     IRDPFVLSWPKQIAARGELRHQFVHACDLLPTLLELIGIEAPGAIAGCPQMAIEGESF
                     ARSIADASAPSKSSPQYFEMFGHRGLWHRGWKAVSYHPPGTPFENDKWELFHLDRDFS
                     EVHDLAEQEKQRLADMIKMWWSEAEKHQVLPLDDRFGPRFAENAARFHGARDHFTFHA
                     GMGHVPTDVAPDVRSRSYTIEAHVEIGENGAEGVLIAHGDATSGYSLYVKDGHLVHDL
                     NIGGGHEIVRSTRKVSVGAHRLGVQVKRLVRKEPPAKGARTGVSEHTLLINGEPAGAL
                     QTLLGFHHLISWSGLDIGRDRGSPVSHYDAPFEFTGKLLRVTVKMHDDQTLDGTGVGT
                     AQMARQ"
BASE COUNT          834 a         1464 c         1372 g          834 t
ORIGIN      
        1 tgcgttcaag ggttccagcg aaatcggctt caccatcgtg tcgatcagcg tttcgctggt
       61 cgcggtgttg atcccgctgc tcttgatggg cggcatcatc gggcgcctgt tccgcgaatt
      121 cgcggtcgtg ctggcgatga ccatcttcgt ctcgatggtg gtgtccctga cgctgacgcc
      181 gatgatggcc tcgcgtttcc tgcgggcgca cagcgagacc cggcatggca ggttctacca
      241 gtggagcgag cgctgcttcg acgccatgct gcacgcctat gagcgcggcc ttgatgtggc
      301 ccttagctgg aagcgaacca cgctgatcgt cttcttcgcg acacttgccc tgtcggtcta
      361 tctcttcgtg atcattccaa agggcttctt tccgcaacag gacaacggcc tgatcaccgc
      421 gacgtcggag gcctcgcagg acatctcctt cgaggcgatg aaggccaagc aggaagaact
      481 ctccaggatc gtgatgcagg atcccgcggt agccacggtt gcaatggcaa tcggcggcag
      541 cggcagggcc ggcaacaacg gcaacctgtt catcacgctg aagccgcgca gagagcgcga
      601 cgcctcggcg cagcaggtca tttcgagact gcgtcccaag ctcgagaagg tcgagggcgc
      661 acgcctctat atgcaggccg cgcaggacgt gcggctgggc ggccgcccga cccgcacgca
      721 gttcgaattc acactgcagg acgccaacct tgccgaattg aacgaatggg cgcccaagat
      781 tcttgccagg atgcagacgc tgccggagtt acgtgacgtc gcgaccgacc agcagaccca
      841 gggcacaacg ctcgaactca agatcaatcg cgacaccgcg tcgcgctacg gaatctaccc
      901 gcagctaatc gacgacacgc tgtacgatgc gttcggccag cgccaggtca cccagtattt
      961 tacgcagctc aacagctatc acgtcatcct cgaaatcctg cccgaactgc aggggcggat
     1021 cgactcgctc gacaagctct acatccgctc gcccaccacc ggcgaccagg tgccgatctc
     1081 gacgtttgcg acctggacca gcgtgccggt tcgtccactg tcgatcagcc accagggaca
     1141 attcccggcg attaccatca gcttcaacct cgcccagggc gtcgcgctgg ggcaggcgac
     1201 cgacgccgtg cagaaggcga tgaccgagct cggggcgccg gccacactca actcgagctt
     1261 ccagggtacc gcgcaggcgt tccagcaatc actcggcacc gtgccgctgc tgatcctcgc
     1321 ggcgctggtc gtggtctacc tgatcctcgg catcctgtac gagagctaca tccatccgat
     1381 cacgattctg tccacccttc cctcggctgg cgttggcgcg ctcgcgatcc tgatggcgtt
     1441 cggctacgat ttcagcctga tcgcgctgat cggcatcatc cttctgatcg gcatcgtgaa
     1501 gaagaacggc atcatgatgg tcgacttcgc gatcgcggcc gaacgcgacg agcatttgag
     1561 ccccgagcaa tcgatccgac aggcggcgct gctgcgtttc cgtccgatca tgatgacgac
     1621 gatggcggca ttgctaggcg gcgtgccgct gatgctggga accggaacgg gatcggaaat
     1681 ccgccagccg cttggctacg cgatggtagg cggcctgatc gtcagccagg cgctgacgct
     1741 gttcacgacg ccggtgatct atctctatct cgacaagctc tccaatgcgt tctcgaattg
     1801 gggccgttcc ggaggagcgc gtgacgatcg cgaacccgag agcgcttccg tcaagcaggc
     1861 cgccgagtga cttgactttg ctggcgctcc ggcacatcgg gatgaagcaa tcccggagcc
     1921 ggttcagtga aaatcatagt caagcttgcc gctgttctag gcgtcgtggc attcggatgg
     1981 acgcctggtc tcgccgagca ggcgccgaag aagaccccgc cccacaccgc ccaacccagc
     2041 acgcagcccg ttgtccccac gcctccgcag tcggacgcgc cggcgacgag cccgccaacc
     2101 ccgccagcgc cgaactgggc ctcgcgctgc accagcccaa gccgtgacgc gcccctcgaa
     2161 tgcgcgatcg aggagactgc ggtcctgacc aagaccggtc agttgatcgt ccttgtcaac
     2221 attcgcgtcg caggcgatgc ccgttcgccg gtcgcgctgg tgcaactgcc gctcggtctc
     2281 aatctgcccg gtggcgccag gctccaggtt gacgacggca aggtcgtcga gctgctgatc
     2341 cagacctgcg agaaccgcgg ctgttacgcc aacacccaaa tcagccccga cctgttggcc
     2401 gcactgaagt caggcaagca gctcaaggtg tcgttccaga atctcgccaa ggaaacgatc
     2461 acgattccca tgccgctcgc cgatttcacc gcggcctacg acaagatcaa ataggcgcct
     2521 cgtcattgcg agaagcgtga gctcggaagc aatccagctt cttttcgtgc ctctgcatgg
     2581 cttcgttggc tcgcaatgaa gtggcaggca tcattgccgc gccatctgcg cggttccgac
     2641 gcccgtgccg tcgagcgtct ggtcgtcgtg catcttcacc gtgacgcgca gcagttttcc
     2701 ggtgaattcg aacggcgcgt cgtagtgcga tactgggctg ccgcgatcac ggccgatgtc
     2761 gagccctgac caggaaatca ggtggtgaaa cccgagtagt gtctgcagcg cgccagcggg
     2821 ctcgccgttg atcagaagcg tgtgttcgct cacaccggtg cgcgcgccct tggccggcgg
     2881 ctccttccgc accagccgtt tgacttggac gccaagccga tgcgcgccca ccgatacctt
     2941 gcgcgtcgaa cgtacgatct cgtggccgcc gccgatgttg aggtcgtgca ccaaatggcc
     3001 gtccttgaca tagaggctgt aacccgaggt ggcatcgcca tgcgcgatca gcacgccctc
     3061 ggccccattt tcgccgatct cgacgtgggc ctcgatggtg taactccggc ttctcacatc
     3121 gggtgcgacg tcggtcggca catgccccat cccggcgtgg aaggtgaaat ggtcgcgagc
     3181 gccgtggaag cgggcggcat tctctgcaaa gcgcggccca aagcgatcgt cgagcggcag
     3241 cacctgatgc ttttccgcct cgctccacca catcttgatc atgtcggcaa gacgctgctt
     3301 ctcctgttcg gcaagatcat ggacctccga aaaatcgcga tcgagatgaa agagctccca
     3361 tttatcattc tcgaacggcg tcccgggcgg gtggtaggag accgccttcc atccgcgatg
     3421 ccagaggccg cgatggccga acatctcgaa atattgcggc gaggattttg acggcgcgga
     3481 ggcgtccgca atcgagcgag caaaactctc gccttcgatc gccatctggg ggcagcctgc
     3541 aatcgcgccg ggcgcctcga ttccgatcag ctccagcaag gtcggcagca ggtcgcaggc
     3601 atggacgaat tggtggcgca gctcccccct cgcagcaatc tgcttgggcc agctcaacac
     3661 aaagggatcg cggatgccac cgccatgggt gttctgcttg tagcggcgca agggcgtatt
     3721 cgacgccatc gcccagccat gcgggaaatt cgagtgcgta ccgggaccgc cgatatcgtc
     3781 gagccggcgc agcttttcgg ggatcgattc gggccggaaa ttgtagggtc ccatcgcatt
     3841 gatgaatccg agcggcccgc cctcctggct cgcgccattg tccgacatca cgatgacaag
     3901 agtgttctcc cggatgcctg cagcttcgag gaaactgatc agccgtgcca gatggcggtc
     3961 ggcgtgatcg agcatgccgg cgaaggccga ttgcaggcgt gtgaacacgc gcttctcgtc
     4021 ggcgctgtgc tcgtcccacg gcttaacgcc gtcattgcgc ggcggcaaac tcgtatccag
     4081 aggcaccagt cccatcgcct tttgccgcgc cagacgttgc tcgcgctcca cgtcccagcc
     4141 gtgctcgaac aaggcatcat agcccctgat gatgtcggcg ggcgcctgat gcggcgcgtg
     4201 gcaggcgccg aacgcaaccc acgtcagcca gggcagatcg ggacggtcgg cgacgtgatc
     4261 gccgataaag cggatcgcat ggtcgaccag atccgacgtc aggtgataac cgccggcgtg
     4321 ggtaccgggc ggatcaatat gcgtgttgtc ggagacgagt tcaggggcat attgatcggt
     4381 ctcggcatcg aggaagccgt agaagcgatc gaagccacgc cccagcggcc agccgtcgaa
     4441 cggcccggtg gcgccgctct cggtcaatgg cgtcacatgc catttgccga ccatgtagtt
     4501 gcgg
//
LOCUS       DATLJT010000105         4246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2991447,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000105 DATLJT010000000
VERSION     DATLJT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991447"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VM910_02335"
     CDS             <1..661
                     /locus_tag="VM910_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVI61425.1"
                     /translation="RVLFEDTQYLQWLANTGFVGVAVVVITLALAVPAGYALARMTGP
                     WAQTLGVAIFLTYLVPPTILFIPFSRIIAMLGLQDSLWSLVLVYPSFTVPFCTWLLMG
                     FFKAIPRDLEDAAMIDGFSRLGAFVRVVLPISYAGLLTAVIFAFTLVTQEFVYGVTFI
                     TASRSYTVSVGVPTFLVRGDVYFWGSMMAACLIASLPIAIIYNFFVDRFVAGFTVGAV
                     K"
     gene            complement(949..1020)
                     /locus_tag="VM910_02340"
     tRNA            complement(949..1020)
                     /locus_tag="VM910_02340"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(1013..1581)
                     /locus_tag="VM910_02345"
                     /pseudo
     CDS             complement(1013..1581)
                     /locus_tag="VM910_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272357.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1965..2438
                     /locus_tag="VM910_02350"
     CDS             1965..2438
                     /locus_tag="VM910_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61426.1"
                     /translation="MPRKSAAQLAAEEASASLPGGKLDRPDPPPDMLEVAAMIWRDTV
                     ASMKPRHFSRETWPLLTRYCNAMALCNQLEVELTRVSIQAPEHGEILKKLNCAAALGL
                     TYARALRLTPKSNVETRGNTRDPHRLMGRRLWEPDYQPGATLRRLWGKDDKTKPS"
     gene            2442..2753
                     /locus_tag="VM910_02355"
     CDS             2442..2753
                     /locus_tag="VM910_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61427.1"
                     /translation="MTDTVVAFPNTPSPPLHLSPKEAEVWRAVMASQKAGHFGPEIFP
                     VLEAYCTTALICDRMAARLRIDESNDRLLASYDEATASLLRLAQALGLLPADEREAMV
                     G"
     gene            2753..2959
                     /locus_tag="VM910_02360"
     CDS             2753..2959
                     /locus_tag="VM910_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61428.1"
                     /translation="MPVKLTDNQLREIQLAAQIVPYDLRSRFLEKVMSELRGKDLGDG
                     LVHRIAYEVARSITWDSERTAVVG"
     gene            3322..3462
                     /locus_tag="VM910_02365"
     CDS             3322..3462
                     /locus_tag="VM910_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61429.1"
                     /translation="MPAFPYSTLDAGFADAGLMIVVIGTVLIALTAHVLAAIGWLRRP
                     RP"
     gene            3459..3692
                     /locus_tag="VM910_02370"
     CDS             3459..3692
                     /locus_tag="VM910_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61430.1"
                     /translation="MTSEEFLEERRQVAREYLDKGDTRSAIFVMFAYAQQAGMDRDDC
                     VRLTQRALTSMSNDIEKVRRFVESWRVVNLDLQ"
     gene            3704..>4246
                     /locus_tag="VM910_02375"
     CDS             3704..>4246
                     /locus_tag="VM910_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61431.1"
                     /translation="MPKNTPAQHFRDLFNRWTSGSTEHECAQGEKAADAWLKRNSKTR
                     LDIPKILAQAVADDAAQQPPPPPPSDPRDAQKHPYEDPQFTPVGLVHGITGKYLTMTP
                     HVHVIYTLFIVATHVYRQFDIAARLALVSERPDSGKSEALKVAKTLMLRPNPEALSTG
                     AAIIDFIVEGPGTIGLDELDL"
BASE COUNT          788 a         1325 c         1283 g          850 t
ORIGIN      
        1 cagggtcctg ttcgaggata cccaatatct gcaatggctc gcgaacacag gcttcgtcgg
       61 agttgccgtg gtggtgatca cgctcgcgct cgcggtgccg gcgggatatg cgctcgcgcg
      121 catgaccgga ccgtgggcgc agacgctggg cgtagcgata ttcctcactt atctcgtgcc
      181 gccgacgatc ctgttcatcc cgttctctcg catcatcgct atgctcggcc tgcaagattc
      241 actgtggtca ctggtactgg tctatccaag cttcacggtg ccgttctgca cttggttgtt
      301 gatgggcttc ttcaaggcga tcccgcgcga tctcgaggat gccgccatga ttgatggatt
      361 ttcccgcctc ggcgccttcg tcagggtggt gctcccaatc tcatatgccg gcttgttaac
      421 cgcggtgatc ttcgccttca ccctagtgac gcaggaattc gtctatggtg tcacgttcat
      481 caccgcttca agaagctaca ccgtaagcgt gggcgtgccg accttcctgg tgcgcggcga
      541 cgtctatttc tggggctcaa tgatggcagc ctgtctgatc gcgagtctgc cgattgcgat
      601 catctacaac tttttcgtcg accggttcgt cgcgggcttc accgtcggcg cggtgaaata
      661 ggaatgagaa cgcgccgggt ggggtccacg gcaggaccgt gctgcctgcg cgccccctcc
      721 gcgccaggga gaccttcacg aaagcggagc gaaacgtggc aacgctgcgg tcggtccgcc
      781 gcacgttcag cctgcctcag aagcgtcgcc cggcgcgaga cgtgcgcgcg cagcctcgct
      841 tggtcaaacg gaattctgtg caagtacgga tagacgctga attttgtcga ttgttaccca
      901 ggccgttacc gggaccggtt tcactatgtc aaaaattacc ctaagccctg gcgcacccga
      961 agagattcga actcctgacc cccagattcg tagtctgatg ctcaaatcaa tctcaggcgc
     1021 tctttgattg tgctcgccgc actcgctcca gcatctcctc gacctcacgt ttggcctgcg
     1081 cgagcttctg tgcatcgcgg gcgcgcagca ccacattcgt gttggccccg tgctgcggat
     1141 cgaaaaacag atagctgccg atcgcgacct cgggattggc tttggcgatc tcaccgagct
     1201 gcagccctcg cgcgtatcgg cgcgcaccgt ttctgagaac atgcgaacgc cgatcttgag
     1261 cttaggcgcc acctcatcga gcatcgcctg catgatcgag gtcacgcccg ccatcacaat
     1321 cacattgccg atccagaagc caggcgcccc cgacaccttg ttgagaatga gatccgcgcc
     1381 cttgggaatg cgcgtcatgc gcagtgcctc gttgatctcg gtgccggtcg tcttcaccca
     1441 ttcgtgcagg atggcgagcg cgcgcggatc gcgatcgatc ggcacacggt ccttggtgcg
     1501 gccggacagg atcttgccgc cgatcaccag gagcgcggcc gtgacaacct ctttggaagc
     1561 ggacgagtcg gcggttggca taatcaccag cgagatgttt aaggggcaag actcctggag
     1621 ctccacgcct gatcaacgcc gggcgtcggc ccttcggacg gctggccgtt ttatcggcct
     1681 attgtgggcg gggggtggtt cgaaaaaaag aaggtgccca tcggtcgggc gcggcggcgg
     1741 ccgacctttg caacactcta caattctcac gatttgaacg ttttctactt caagttgtat
     1801 ttttagagtt ccatgaaagg ttctaacttt aggacctttt ggagccaaaa gacgcaaaat
     1861 cggggccttt tcagccgccc gcccaggccg gttccgcccc cctcgctctc gccaattcgc
     1921 cggttccggg gcatgatggc ggcgccctcc aggaggctgc cccgatgccc cgaaaatccg
     1981 ctgcccagct tgccgccgaa gaagcgtcag catcactgcc cggtgggaag ctcgaccgac
     2041 cggatccgcc tccggacatg cttgaggtcg cagctatgat ctggcgcgac accgtcgctt
     2101 cgatgaagcc gcgacacttc tcccgtgaga cctggccgct gctcacccgt tattgcaatg
     2161 cgatggcgtt gtgtaatcag cttgaggtcg agctgactcg cgtcagcatt caggcgcctg
     2221 agcacggaga gatcctgaag aagctcaatt gcgccgccgc cctcgggctc acctacgccc
     2281 gcgcgctccg gctcacaccg aagtctaatg tcgagacccg cggcaacacg cgcgatccgc
     2341 atcgcctcat ggggcggcgg ctgtgggagc cggactacca gcctggcgcc acgctgcgcc
     2401 ggctctgggg caaggatgac aagaccaagc cgagctgagc catgacagat actgttgtcg
     2461 ccttcccgaa caccccgtca ccgccgctgc acctctcgcc gaaagaggcc gaggtctggc
     2521 gcgcggtcat ggcgtcgcag aaggccggac atttcggccc cgagattttc ccggtgctgg
     2581 aggcgtactg cacgaccgcg ctgatctgtg atcgcatggc ggcgcgatta cgcatcgacg
     2641 agagcaacga ccggctgctc gcgagctacg acgaggcgac ggcttcgctg ctgcgcctcg
     2701 cgcaggcgct ggggctgctg cccgcggacg agcgggaagc gatggttggc tgatgccggt
     2761 caagctcacc gataatcagc tccgcgaaat ccaactcgcc gcccagatag tgccctacga
     2821 cttgcgatcg cgcttcctcg aaaaggtgat gagcgaactg cgcggcaagg atctcggtga
     2881 tggcctcgtc catcgcattg cctacgaggt ggcgcgctcg atcacgtggg acagtgagcg
     2941 gacagcggtg gtgggttagg cggcgagttt ttcaaaccta gcactgctaa ggtttgatcg
     3001 cccgacacga agcagtttcg gaaattttgg gtcgcacaga cggctgccgg tgactgcctt
     3061 aattgagggt cacctcggtc cggctcatca aaacaaacgc accagcgggc tccgcacggg
     3121 gtcgatctcg gagggtcagc ggaaattctt aagtgtttcc ggtggcggtc cggctcggtg
     3181 gaaaaaactc gagtttttcg gcgaggtgcg ttttccgctt gatttcgcgg tcgtggttag
     3241 gttcgatgtg aaagcgcccc cggcggaagt tgcagcaacc gccggggact tggcgcagta
     3301 aaccggtatc agcggttcac catgcccgct ttcccctata gcacacttga tgctggcttc
     3361 gccgacgcgg ggctgatgat cgtcgtcatc ggtacggtgc tgatcgcgct cactgcccac
     3421 gtcttggcgg cgatcggctg gctgcggagg ccgcggccat gacctcggaa gagtttctgg
     3481 aagagcgcag gcaggtcgct cgcgaatatc tcgataaggg cgatacgcgg agcgccatct
     3541 ttgtgatgtt tgcttacgcg caacaggccg gcatggaccg cgacgactgt gtgcggctga
     3601 cgcagcgcgc cctcaccagc atgagcaatg acatcgaaaa ggtgcgccgg ttcgtcgaga
     3661 gctggcgtgt cgtcaatctt gaccttcagt aacgggcctg cccatgccga agaatacgcc
     3721 agcccagcac ttccgcgatc tcttcaaccg ctggacatcc ggctcgacag agcacgaatg
     3781 cgctcaaggc gaaaaggccg cggacgcgtg gttgaaacgg aacagtaaaa cccgcctcga
     3841 tattccgaag atcctggctc aggccgttgc cgatgacgcc gcgcaacagc ccccgccgcc
     3901 gccgccgtcc gatccgcgcg acgcgcagaa gcatccgtat gaggacccgc agttcacgcc
     3961 ggtcggattg gtgcacggca tcaccggaaa atatctgacg atgacgccgc acgttcacgt
     4021 catctacacg ctcttcatcg tcgccacgca cgtctatcgc caattcgaca ttgctgcgcg
     4081 cctagcgctg gtcagcgagc ggcccgacag cggcaagagc gaggcgttaa aggtcgctaa
     4141 aaccctgatg ctgagaccca atccggaagc gctcagcacg ggggcggcaa tcatcgactt
     4201 catcgtcgag ggtccgggca caattgggct cgacgaactc gatctc
//
LOCUS       DATLJT010000106         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3910528,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000106 DATLJT010000000
VERSION     DATLJT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910528"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VM910_02380"
     CDS             <1..585
                     /locus_tag="VM910_02380"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVI61432.1"
                     /translation="RRRRNLIVVASSILLIALMGALTYYSVPLYRLFCQATGLGGTTQ
                     VARAARGAVPGLAITVRFDANVASNLPWRFNAPEPVEVRLGEQHQVEYTAVNLGNEPV
                     LGMAIYNVTPLEIGKYFYKIQCFYFTEQLLMPGERKEFPVLFYVDPRVADDADGQHVN
                     TITLSYTFYNKGREALERYLRDHHMAALPSPAAR"
     gene            622..1434
                     /locus_tag="VM910_02385"
     CDS             622..1434
                     /locus_tag="VM910_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI61433.1"
                     /translation="MTELAPHQRPKRFPGLVAWLLVLLILLAVVGAWFAFHPGFRWGQ
                     SGAHWAAAMPEGEFERRVRAYLLEHPEVIAEAISRLESQQGAQDAAEVQAVLKSRADE
                     IFRDPDSPVGGNPDGDVTLVEFFDYNCPYCRQMVPVMAQAESADPQLRIVYKEFPILG
                     PNSKLAAKAALATRKQGKYLAFHKALYQVRGTVDPSKVAEVAATVGLDVDRLKAEMDD
                     PAIAALIEKNLALAQALRIDGTPGFVAGHQILRGAVDLKALQGLIQEARGNP"
     gene            1704..1964
                     /locus_tag="VM910_02390"
     CDS             1704..1964
                     /locus_tag="VM910_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61434.1"
                     /translation="MNQCSMMHGDMGALFGSRVTPVMNLSPEDMRGYLGLQLQRLNNK
                     RLKIGDLRSDDGTITADIVTVDNSLVQRLKIDRHTGAIQYEN"
     gene            2100..>2576
                     /locus_tag="VM910_02395"
     CDS             2100..>2576
                     /locus_tag="VM910_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HVI61435.1"
                     /translation="MKTATLDVGGMLSLLDYQAVENQLGRIPGVQRATASVASNSATV
                     EYDETVTSVAALKAKINECGFHCTGQIMPKHVCEPRPIDEHGRHIGHTAHEPAAARAA
                     ATAAVPMSHDMAHEMGHGAGMDMQAMVRDMRNRFWISLVFTVPIFIYSPMGNMFTPP"
BASE COUNT          533 a          789 c          804 g          450 t
ORIGIN      
        1 cgccgtcgtc ggaacctgat cgtcgtcgcg agctcgatcc tcctgatcgc gctcatggga
       61 gcgctgacct attattccgt tccgctttat cgcctgttct gccaagccac cggattggga
      121 ggaacgacgc aggtcgcacg cgcggcgcgc ggcgccgttc ccggtctcgc gatcacggtg
      181 cgtttcgacg ccaacgtagc atcgaacctt ccatggcgct tcaacgcacc cgagcctgtt
      241 gaagttcgtc tcggtgagca acaccaggtc gaatacacgg ccgtcaatct gggcaatgaa
      301 cccgttctcg gtatggcgat ctacaacgtg accccgctcg agatcggcaa gtacttctac
      361 aagatccaat gcttttattt taccgagcag ctcttgatgc cgggagaacg caaggagttc
      421 ccggttctct tctacgtcga tccgagggtc gccgacgacg ccgatggtca gcacgtcaat
      481 acgattacgt tgtcctacac gttctacaat aaagggcgcg aggcactgga gcggtacctg
      541 cgagaccatc acatggccgc gcttccttcg cccgcggccc gctgatccgc aaccctcagg
      601 cccatgggcc gggagactcc gatgaccgaa ttagcgccgc atcagcgacc gaagcgtttc
      661 ccagggctcg ttgcgtggct gctcgtgctg ctgatcctgc ttgctgtggt cggagcatgg
      721 ttcgcattcc accccggttt ccgatggggg caaagcggcg cccactgggc tgccgccatg
      781 ccggaaggtg aattcgagcg gcgcgtgcgc gcatatctgc tcgagcatcc cgaggtcatt
      841 gcggaggcga tcagtcgtct cgagtcgcaa caaggcgcgc aggacgcggc ggaagtgcaa
      901 gcggtgctga agtcgcgcgc cgacgaaatc tttcgcgatc ccgacagccc ggtcggcggc
      961 aatccggacg gagatgtcac gctcgtcgag ttcttcgact acaactgccc ctattgccgg
     1021 cagatggtgc cggtgatggc gcaagcggag agcgcggatc cccagctacg catcgtctac
     1081 aaagagttcc cgatcctcgg acccaattcc aagcttgcgg ccaaggccgc gcttgcaacc
     1141 cgtaaacagg gcaaatacct cgccttccac aaggcgctct atcaagttcg cggaaccgtg
     1201 gacccgagca aagtcgcgga agtcgccgcc acggtcggcc ttgacgtgga tcgactgaaa
     1261 gccgagatgg acgatcccgc gatcgcggcg ctgatcgaga aaaatctagc gctcgcgcag
     1321 gcattgcgga tcgatgggac accggggttc gtggccggcc accagatcct tcgcggcgcg
     1381 gtcgacctga aagcacttca gggattgatc caggaagcaa gaggcaaccc ctgatggacc
     1441 aggcacggct ggcaggctca gacaacggaa tgaaacaaag gttcgccaac agctagatca
     1501 agaggagaag cacatgaaga caaggacctt tcttttggtg tcagcgatgg cgggcgcgct
     1561 cgcggtcggt gcggttgacg cggcgagcgc acaaggcatg caagacagga tggggactgg
     1621 catgatgcag ggtgggccga gccagggggc tggcggcggg attgatgcgg ggcgggatgg
     1681 gttcgtcggc cacagagtcg ggcatgaatc agtgcagcat gatgcatggc gacatgggtg
     1741 cgctattcgg gtcgcgcgta acgccggtca tgaatctatc gcccgaagac atgcgcggct
     1801 atctcggctt gcaacttcaa cgcctgaata acaaacgtct gaagatcggg gacctcagaa
     1861 gcgacgacgg aacgatcacc gccgatatcg tcaccgtcga caactcgctt gtgcagcggc
     1921 tgaagatcga ccggcacact ggtgcgatcc agtatgagaa ctgagcatac gcggcagcca
     1981 ggtgatcgcg gtgcgccttt gcaggcaggc tgactgcctc cgcccgaccc gaagggccgc
     2041 gacgtcgctt ccaaagtgaa agcggcaagg tcggccgaca tgagtgagag gaacgaggca
     2101 tgaaaacagc cactcttgac gttggcggca tgctgtcctt gctggactac caagccgtcg
     2161 agaaccagct cggccggata cccggggtcc aacgcgcgac cgccagcgtc gcctcgaaca
     2221 gcgcgacggt cgagtatgat gagaccgtca ctagtgtggc ggcgctcaag gccaagatca
     2281 acgagtgcgg cttccactgc accggccaga tcatgcccaa gcatgtctgc gaaccacgtc
     2341 ccattgacga gcacggcagg catatcggtc acaccgcgca cgaaccggcc gcggcccgcg
     2401 ctgcggcaac agccgctgtg ccaatgtcgc acgacatggc gcacgagatg ggccacggcg
     2461 ccggcatgga catgcaggcc atggtgcggg acatgcgcaa ccgcttctgg atcagcctcg
     2521 tgttcacggt gccgatcttc atctactcgc ccatgggcaa catgtttacc ccaccg
//
LOCUS       DATLJT010000107         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2531980,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000107 DATLJT010000000
VERSION     DATLJT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531980"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VM910_02400"
     CDS             complement(<1..367)
                     /locus_tag="VM910_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HVI61436.1"
                     /translation="MSTVSISLALAIRVVLVLLFLPFSALDKVLDFRSAVAQAREAVP
                     NKAAATALILVGLFVEVFMSAGVVSGIADRACAFVLAGYCAVTALVWKQFWRPGDFWS
                     SSDGKARALFWDFWKNLALA"
     gene            complement(460..2013)
                     /locus_tag="VM910_02405"
     CDS             complement(460..2013)
                     /locus_tag="VM910_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVI61437.1"
                     /translation="MDQRFDVIIIGSGAGGGTLARHLAPSEKRILVLERGDWLKREAE
                     NWDAAAVFVDNRYVSPETWYDRDGRPFQPQVHYFVGGATKLFGAALYRLRRQDFGELK
                     HYDGISPAWPISYEELEPYYAKAEEIYHVHGLRGHDPTEPPASGPYPCPPVSNEPRIQ
                     ELFEQLKAAGCRPFPAPCAIMLDERKMAYSTCIRCQTCDGFPCLVHAKSDAEVVAVRP
                     ALEFPNVTLLRNAKAVKLETNAAGTAVTEVIAEVAGEREVFRGDIVVVSCGAVNSAKL
                     LLISRNEKHPDGLANGSDQVGRNYMFHNSQAVLAISREPNPTMFQKTIALNDFYFGMD
                     GFEYPMGNIQMTGKSLGPMYRGEKPLETGLFPLGLLDDLARHSVDFWLTTEDLPDPDN
                     RVTVDASGMVTLSYTPNNQVPQQKLYAKLKSMLSHLGMHPEHLIPRNIYMKTAIPIAG
                     CAHQAGTCRFGTNPKTSVLNTNCKAHELDNLYVVDTSFFVSIGAVNPSLTAIANAIRV
                     GDHLLQRLA"
     gene            complement(2019..>2523)
                     /locus_tag="VM910_02410"
     CDS             complement(2019..>2523)
                     /locus_tag="VM910_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI61438.1"
                     /translation="NNAGIETRTSVLDTTESQYDRVMAVNLKSAFFGTQIAARQMIKQ
                     GGGGRIINITSVHEDWPMPGNTPYCLAKGGMRLLTRTAGLELAKHNILVVGVGPGAVA
                     TPINISTMNDPAKLAQLNAAIPLGRMARPEEIASVVGFLAGEGASYLTATTVFADGGI
                     MHSSVGL"
BASE COUNT          502 a          796 c          742 g          483 t
ORIGIN      
        1 cggccaaagc caagttcttc caaaaatccc agaacagcgc gcgggccttg ccgtcgcttg
       61 aactccaaaa gtcgccaggg cgccagaact gcttccaaac tagagccgtc accgcacagt
      121 agccagcaag cacgaatgcg caggcacgat ccgcaatccc ggaaacaacc cccgccgaca
      181 taaacacttc gacaaataaa ccaaccagaa tcaatgcggt tgccgccgcc ttattcggca
      241 ccgcctctcg agcctgcgcc acagcactgc ggaagtcgag gaccttatcc agggcgctaa
      301 acggcagaaa caggagaacg agaactaccc ggattgctag agcgagactg atactaaccg
      361 tcgacatgtg cacccccatg ctgtcgcgac ttgggctatg agatccccca acttagcaga
      421 aaccgcgttg ccgcatgccg atcattagcg gctgcctgct tacgctagcc tctgcaatag
      481 gtgatcgccc acccggatcg cattcgcaat cgcggtcagc gacgggttca ccgcgccgat
      541 gctgacgaag aagctcgtgt cgaccacgta gagattgtcg agctcatgcg ccttgcaatt
      601 cgtgttcagc accgaggtct ttggatttgt gccgaagcga catgtgccgg cttggtgagc
      661 gcagccggca attgggattg cagtcttcat atagatgtta cgcggtatca ggtgctcggg
      721 atgcatgccg agatggctca gcatcgactt gagcttcgcg tagagcttct gttgtggcac
      781 ctggttgttc ggggtgtaac tcagcgtcac cataccggac gcatccacgg tgacgcgatt
      841 gtccggatcg ggcaggtcct ccgtcgtgag ccagaaatcc accgaatggc gcgcgagatc
      901 gtccagtaga ccgagcggga acagcccggt ctcgagcggc ttttcccccc ggtacatggg
      961 acccagcgac ttgcctgtca tctgaatgtt gcccatcgga tattcgaatc cgtccatccc
     1021 gaaatagaaa tcgttgagcg ctatcgtctt ctggaacatg gtcggattgg gctcgcgcga
     1081 gatcgcgagc acggcctggc tgttgtgaaa catgtaattg cgccctacct gatccgagcc
     1141 gttagccaac ccatcggggt gcttctcgtt gcgtgatatc agcagcaact ttgccgaatt
     1201 caccgccccg caggagacca caacgatatc gccgcgaaag acctctctct cgcccgcgac
     1261 ttcggcgatc acttcggtga ccgccgtgcc cgccgcattc gtctccagct tcaccgcttt
     1321 cgcgttgcgc agcagcgtca cgttgggaaa ctcgagggcg ggacgcacgg ccaccacctc
     1381 tgcgtcggat ttggcgtgca ccaggcaagg aaagccgtcg caggtctgac agcggatgca
     1441 ggtgctgtac gccatcttcc gctcgtccaa catgatcgcg caaggagctg ggaacgggcg
     1501 gcagccggcc gccttaagct gctcgaacag ctcctgaatg cgcggctcgt tggagaccgg
     1561 cggacatgga tagggcccgc tggcgggagg ctcagtcggg tcgtgcccgc gcaagccgtg
     1621 gacgtggtaa atctcctcgg ccttggcata atacggctcc agctcctcgt agctgatcgg
     1681 ccatgccggc gagatgccgt cgtaatgctt gagctcgccg aaatcctgtc gccgcaagcg
     1741 atagagcgcg gcgccgaaca gcttggtcgc accgccgacg aaataatgca cctgcggctg
     1801 aaacggtcgg ccatcgcgat cgtaccaagt ctcgggcgac acatagcggt tatccacgaa
     1861 caccgccgcg gcgtcccagt tctccgcctc gcgcttgagc cagtcaccgc gctccagcac
     1921 gaggatgcgc ttttccgaag gagcgagatg cctcgccaga gtcccgccgc ccgcgccgct
     1981 gccgatgatg atgacgtcga agcgctgatc catacccgct agagaccgac gctgctgtgc
     2041 atgatgccgc cgtccgcgaa gactgtggtc gcggtgaggt agctggcgcc ctctccggca
     2101 aggaacccga ccacgctagc aatctcctcc gggcgcgcca tgcgtcccag cggtatggcg
     2161 gcattgagtt gggcgagctt ggccggatcg ttcatcgtgc tgatattgat tggcgttgcc
     2221 accgcgccgg gaccaacgcc aaccacgagg atgttatgct tggcgagctc aagacccgcg
     2281 gtccgcgtga gcaggcgcat gccgcccttg gccaggcagt agggggtgtt gcctggcatc
     2341 ggccaatcct cgtgcaccga cgtgatattg atgatgcggc caccgccgcc ctgcttgatc
     2401 atctgccggg ctgcgatttg cgtaccgaaa aatgcactct tgaggtttac ggccatcacc
     2461 cggtcatact gggactcggt tgtgtccaga accgaagtcc gcgtctcgat gccggcgttg
     2521 ttg
//
LOCUS       DATLJT010000108         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2991504,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000108 DATLJT010000000
VERSION     DATLJT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2991504"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VM910_02415"
     CDS             <1..408
                     /locus_tag="VM910_02415"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVI61439.1"
                     /translation="AGSMLPFTVFDADGRISGMTTYMNVDAPNRRVEIGSTWYARRVQ
                     RSALNTQCKLLLLTHAFEKLDCIAVEFRTHFFNHQSRRGIERLGAKQDGILRSHQIAP
                     NGTLRDTVVYSIIASEWPTVKAHLAYQLHDRQR"
     gene            469..2115
                     /locus_tag="VM910_02420"
     CDS             469..2115
                     /locus_tag="VM910_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI61440.1"
                     /translation="METFDYVIIGSGSAGSVLTNRLSEDAATTVCVLEAGPSDWHPYI
                     HLPAGFIKTFHMRSINWAYQQEPGPYTGGRSIYAPRGKTLGGSSSINGHIYNRGQRLD
                     FDTWAQLGNRGWGYADVLPYFKRMERRIGEGDDAYRGRDGALTVTTMEWKDPLCEAFM
                     AGAMSLGIPRNHDYNGAIQEGVSYAQRTIKNGLRVSAATAFLYPARRRPNVHVRTHAH
                     ATQIILEGKRAVGVRYMKGGRNGTPVEVRANREVILSGGSYNSPQLLQLSGIGTPELL
                     RSHGIEVRHALPGVGEGLQDHYAPRSVARVKNIKTINELRQGWHLWVEALKWATTRRG
                     LLSLSPTMVYCFWHSGETTESSDLQLTFTPASYKEGVQGQLEDKPGMTIASWQQRPES
                     RGYVRIRSADPFAPPIIQTNYLTAELDRRVVVAGMKLARRLLKSVPLSPYYEYEDFPG
                     PNVQSDDEFLRAATERGTTTFHPGCTCRMGPADSTWAVVDDQLRVHGLQGLRVIDASI
                     MPRMISANLNASTLMIADKASDLIRGRRAPEAIVTATAAE"
BASE COUNT          476 a          737 c          746 g          406 t
ORIGIN      
        1 gcgggatcga tgctgccgtt caccgtgttc gacgccgacg ggcgaatttc cggcatgacg
       61 acttacatga acgttgacgc gccgaaccgg cgggtcgaga tcggctcgac ctggtacgcc
      121 aggcgtgtgc agcgcagcgc gctcaataca caatgcaaat tgctgctgct cacgcacgcc
      181 ttcgagaagc tcgattgcat tgcggtagag ttccgtactc atttcttcaa tcaccagagc
      241 cgccgtggta tcgaacggct gggtgccaag caggacggca ttttgcgcag ccatcagatt
      301 gcaccaaacg gcacgctgcg cgacactgtg gtctacagta tcatcgcgtc cgaatggccg
      361 acggtgaaag cgcatctcgc ctatcaactg cacgacaggc agcgctgaat ttgaagtcgg
      421 ctcgcttcga gggcagcgag tcgaagtgat aaacaagaag aaagtgcaat ggaaacgttc
      481 gattatgtga tcatcggctc gggctcggcc ggcagcgtgc tcacgaaccg cctgagcgag
      541 gacgcggcga cgacggtttg cgtgctcgag gccggtccca gcgattggca tccctacatc
      601 catttgccgg cgggcttcat caagaccttt cacatgcgca gcatcaactg ggcctaccag
      661 caggagcccg gtccttacac cggcggccgc agcatttatg cgccgcgcgg caagacgctc
      721 ggcggatcgt cgtcgatcaa cggccatatc tacaatcgcg gccagcgcct ggatttcgac
      781 acctgggcgc agctcggcaa tcgcggatgg ggctatgcgg acgtgctgcc ctatttcaag
      841 cgcatggaac gacgcatcgg cgagggcgat gacgcctatc gcgggcgcga tggcgcgctc
      901 accgtcacca ccatggagtg gaaggatccg ctgtgcgaag ccttcatggc cggcgccatg
      961 agtctgggca tcccgcgcaa ccacgattac aacggcgcga tccaggaggg cgtctcctac
     1021 gcccagcgca cgatcaagaa cggtcttcgc gtcagcgcgg caacggcgtt tctgtacccc
     1081 gcgcgcaggc gaccgaatgt tcatgtgcgg acgcatgcgc atgcgaccca gatcatcctc
     1141 gagggcaagc gagcggtcgg cgtgcgctac atgaaaggtg gtcggaacgg cacgccggtg
     1201 gaggtgcgcg ccaacaggga agtgattctg tcaggcggct cctacaactc gccgcagctc
     1261 ctgcaattgt caggcatcgg tacgccggag ctgttgcgct cgcacggcat cgaggtgcgt
     1321 cacgcgctgc cgggtgtggg cgagggcctg caggaccact acgcgccgcg ttccgtcgct
     1381 cgtgtcaaga acatcaagac catcaatgag ctcaggcagg gatggcatct ttgggtcgaa
     1441 gccctgaaat gggcgaccac acgacgaggg ctgctctcgc tgtcgccgac catggtctat
     1501 tgcttctggc actccggcga gactaccgag agctccgacc tgcagctcac cttcacgccg
     1561 gcaagctaca aggaaggcgt gcagggccag ctcgaggaca agcccggcat gacgattgcg
     1621 tcctggcagc agcggccgga aagccgtggc tatgtccgga ttcggtcggc cgatccgttt
     1681 gcgccaccga tcatccagac caactatctc accgccgagc tcgatcgccg cgtcgtcgtc
     1741 gcgggcatga agctggcgcg gcggctgctg aagtcggtgc cgctgtcgcc ctattacgaa
     1801 tacgaagatt tcccgggacc caacgtacag agcgatgacg aattcctgag ggccgcgacc
     1861 gagcgcggca ccacgacatt ccatcccggc tgcacctgcc gcatgggtcc ggccgacagc
     1921 acctgggccg tggtcgacga ccagttgcgc gtgcacgggt tacagggatt gcgggtgatc
     1981 gatgcctcga tcatgccgcg tatgatctcg gccaatctca acgcctctac cttgatgatc
     2041 gcggacaagg cgagtgacct gatccgcggc agaagagcgc cggaagcgat tgtcaccgcc
     2101 accgccgccg aatgagcgac ccatccgtcc gatgcgaagg gcaggacgat gaacgacgcg
     2161 ggccgcaaag gcggccccgc gaagtcgcag cgacatttgg gttctagggc gtgtactcat
     2221 aaatccgccc gtacagatac ggcgtaggat gtccgcttta ccctcaaaag cgaacagagg
     2281 cgcgagagca tgtccgctat gggccaatag ccgacatcac tcgatcacct cgtcggcacg
     2341 ggcgagcagc gcgggcggca cggtg
//
LOCUS       DATLJT010000109         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3451266,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000109 DATLJT010000000
VERSION     DATLJT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3451266"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..840)
                     /locus_tag="VM910_02425"
     CDS             complement(31..840)
                     /locus_tag="VM910_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HVI61441.1"
                     /translation="MNNLHRELAPISDAAWADIEEETTRTLKRYLAGRRVVDVQGPGG
                     VSLSAVGTGHLKTIAEPGKGILARQREVKALVELRVPFELNRQQIDDVERGANDSDWQ
                     PTKDAAQKIAYAEDRAIFEGYPAAGIGGIRQGTSNPIMTLPADVRHYPDAIARALNQL
                     RLVGVDGPYSVLLSAEAYTALAETSDNGYPVLEHVKQLVKDEIIWTPAIAGAFVLTTR
                     GGDFDLHIGQDLSIGYLSHTDSVVRLYLQETLTFLLLTTEAAVALAPPAKE"
     gene            complement(833..1876)
                     /locus_tag="VM910_02430"
     CDS             complement(833..1876)
                     /locus_tag="VM910_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HVI61442.1"
                     /translation="MVVAQPVSAPLTRAAIFLVVTINPGSDNRAAVRSFCADLPALIR
                     AVDFRDLEARLSCVMGIGSDAWDKLFGEPRPAELHPFREIRAGPRHAVSTPGDLLFHI
                     RAKRMDLCFEMATQIMARLGAAVSTADEVHGFRYFDDRDLLGFVDGTENPRGEAVIDA
                     AIIGEEDAAFVGGSYVIVQKYLHDLDGWNALSTEAQERIIGRAKLSDVELDDSIKPTS
                     AHNALTTITENGKEFQIVRDNMPFGRAGTGEFGTYFIGYCRTPRVTEQMIENMFVGRP
                     PGNYDRLLDFSRAVTGTLFFVPSETFLESVNDDPAAAIRDPQSTPEPAPPGPGAPDGS
                     LAVGSLKGENRHE"
     gene            complement(2101..>2680)
                     /locus_tag="VM910_02435"
     CDS             complement(2101..>2680)
                     /locus_tag="VM910_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVI61443.1"
                     /translation="GSTGFGRAYRDRLQLNWGVTDVDDCVAGARFLAAQGRVDGKRCV
                     ISGGSAGGYTTLAALTFRDFFQGGASYYGVSDIAALARDTHKFESRYLDWLIGPYPQE
                     EARYRERSPLYHADRLAKPVIFFQGEEDAVVPPNQAESMVEALRRKGNPVGYFLFSGE
                     QHGFRKAGNIQRCLDAELAFYAIEVFRTGLTF"
BASE COUNT          476 a          846 c          866 g          492 t
ORIGIN      
        1 atacaccatt ggcgaatggc tttaggtcgt ctactccttt gccggaggcg ccagcgcgac
       61 ggcggcctcg gtcgtgagca gcaggaacgt caacgtctcc tggaggtaga ggcgcacgac
      121 gctgtcggtg tggctcaaat agccgatcga gaggtcctgc ccgatgtgca ggtcgaaatc
      181 gccgccacgc gtggtgagca cgaaggcacc ggcgatggcc ggggtccaga tgatttcgtc
      241 cttcacgagc tgtttgacat gctccagcac gggatacccg ttgtcgctgg tctcagcgag
      301 cgccgtataa gcctcggcgc tgagcagcac tgaatagggc ccgtcgacgc cgacgaggcg
      361 caactggttc aaggctcgtg cgatggcgtc gggatagtga cggacgtcag cgggcagcgt
      421 catgatcggg ttgctggtgc cctgccggat gccgccgatg cctgccgcgg gatagccctc
      481 gaagatggcg cggtcctcgg cataggcaat cttttgcgcg gcgtccttcg tcggttgcca
      541 gtcggagtcg ttcgcacccc gttccacgtc atcgatctgc tgacgattga gctcgaacgg
      601 aacccgcaac tcgaccagcg ccttgacctc gcgctgccgc gcaaggatgc ccttgccggg
      661 ctccgcaatc gtcttgaggt ggccggtgcc gacggcggaa aggctcacac cacccggccc
      721 ctggacgtcg accacgcgcc gcccggcgag gtagcgctta agcgtgcgcg tcgtctcctc
      781 ttcgatatct gcccacgctg catcggaaat cggggcgagc tcgcggtgaa gattattcat
      841 gccggttttc tcctttaagc gagcctactg cgagcgaacc gtcgggtgcc ccaggacctg
      901 gaggtgcagg ctcgggcgtt gattgcggat cgcgtatcgc cgctgcaggg tcgtcgttca
      961 cggattccag gaatgtctcg gatggcacga agaacagggt gccggtcacc gcccggctga
     1021 agtcgagcag tcgatcatag ttgccgggcg ggcggccgac gaacatgttc tcgatcatct
     1081 gctcggtcac ccgcggcgtg cggcaatagc cgatgaagta ggtgccgaat tcgccggtgc
     1141 cggctcggcc gaagggcatg ttgtcgcgta cgatctgaaa ctctttgcca ttctcggtga
     1201 tcgtggtgag ggcgttgtgc gccgaggtcg gttttatcga gtcgtccagt tcgacgtcgg
     1261 acaacttggc gcggccgatg atgcgctcct gcgcctccgt ggacagcgcg ttccatccgt
     1321 ccaggtcgtg gagatatttc tgcacaatca cgtagctgcc gccgacgaac gcggcatcct
     1381 cctcgccaat gatcgcggcg tcgatcactg cctcgccacg aggattctcg gtgccgtcga
     1441 cgaaccccag gaggtcgcgg tcgtcgaagt aacgaaagcc gtggacctcg tcggccgttg
     1501 acaccgcggc acccaggcgc gccatgatct gcgtcgccat ctcgaagcaa aggtccatcc
     1561 gcttcgcgcg gatgtggaac agcaggtccc ccggcgtgga aaccgcatgg cgcggcccgg
     1621 ccctgatctc ccggaacggg tggagttcgg ctgggcgtgg ctccccgaac agcttgtccc
     1681 aggcgtcgga cccgatcccc atcacgcagg aaaggcgcgc ttcgaggtcg cggaagtcca
     1741 ccgcccgaat tagcgccggg aggtctgcac aaaacgatcg cacggccgcc cgattgtcgg
     1801 accccgggtt tatggtcacg accaggaaaa tcgccgcgcg ggtgagcggt gccgatacgg
     1861 gctgtgcaac aaccatagcg tcaatctacg agaagtcttc cgaattggca ttccgcggac
     1921 gtggcccgat ggccccgcgc gattttgcat caaaactgaa agattccaac cccattccga
     1981 cccgtcgaac gtgcaacgtg gatcgcatct ctgaagccga gttggcgacc aggcaaaaac
     2041 caagttcgat gatcgcttca tcgctgacgt acgtggaaag atcgccttgg ggtgggcgcg
     2101 ctagaacgtg agcccggtgc ggaagacttc gatcgcatag aaggcgagtt ccgcgtcgag
     2161 gcagcgctga atgttgccgg ccttgcggaa gccatgctgc tcgcccgaga acaggaaata
     2221 tccgaccgga ttgcctttgc gccgcagcgc ctcaaccatg ctttccgcct ggttcggcgg
     2281 cacgacggcg tcctcctccc cctggaagaa gatcaccggc ttcgcgagcc gatccgcgtg
     2341 ataaagcggc gagcgttcgc gatagcgcgc ctcctcttgc ggatagggtc cgatcagcca
     2401 gtcgagatag cgcgactcga acttatgggt gtcgcgcgcg agcgcggcga tatcgctcac
     2461 gccgtaatag ctggccccgc cttgaaagaa gtcgcggaag gtcagcgccg ccaaggtcgt
     2521 gtagccgccc gcgctgcccc cgctgatgac gcatcgtttg ccgtcgaccc gcccctgcgc
     2581 cgccaggaag cgcgcgccgg cgacgcaatc gtcgacgtcg gtcacgcccc aattgagctg
     2641 cagacggtcg cggtaggcgc ggccgaatcc ggtgcttccg
//
LOCUS       DATLJT010000110         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3680747,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000110 DATLJT010000000
VERSION     DATLJT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3680747"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VM910_02440"
     CDS             complement(<1..666)
                     /locus_tag="VM910_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI61444.1"
                     /translation="MPYGQPPVTRRLEQGKFNRFLAALPPHDFSLLAPHLRTIPLQRG
                     VMLHDVGEEIEHVYFPHTGMVSMVAVMQSGATVETATVGRGGVIGASAALGARSTFGR
                     AIVQSPGSAAWLSAAQFHASASECQTIRDLVVRYNDLLLAQVQQSVACNALHALEARL
                     CRWLLQTHDCVDGDAIPLTQEFLGQMLGVRRTTVTIAARLLQSAGLIRYRRGLIHIVD
                     RAAL"
     gene            complement(848..1426)
                     /locus_tag="VM910_02445"
     CDS             complement(848..1426)
                     /locus_tag="VM910_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61445.1"
                     /translation="MPLYFFHLSFGDRTVPDDEGVELPNRSAARNEALAVIRDLADPE
                     VAGNPRRWASWFLQVVDEGGEFLRLPIGHPALEVVTSEGQQPQAESSEMKPTQPAATV
                     ATRQGRRSAALVQQMSAVRKQTEQLLQRNRQLQHELSSVLAVSANIQIRARHLVAHAR
                     FAALPTHDVDAYRAANKPPRRSPPHLVLLPRE"
     gene            1596..1859
                     /locus_tag="VM910_02450"
     CDS             1596..1859
                     /locus_tag="VM910_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61446.1"
                     /translation="MRSKSDHAWRMAAQCAKLALGADEKDEREFYVRMRDSWITVANR
                     YEFLDGIDEQGAPMKRPRSPAGQYIPYPVSRSLERRRGPVARR"
BASE COUNT          514 a          772 c          837 g          469 t
ORIGIN      
        1 gagcgccgcg cgatcgacga tgtggatgag accgcggcgg taacggatga gtcctgcgct
       61 ctgcagcagg cgggccgcga tcgtcaccgt cgtgcggcgc acgccgagca tctgcccgag
      121 aaactcctgc gtcagcggga tcgcgtcgcc atcgacgcaa tcgtgggttt gcagcaacca
      181 gcggcaaagc ctcgcctcga gcgcatggag cgcattgcac gcgaccgact gctgaacctg
      241 ggcaagcagc agatcgttgt agcgcacgac gaggtcgcga atggtctgac attcactggc
      301 agaggcgtga aactgggcag ccgagagcca cgcggccgag cccggcgact gcacgatcgc
      361 cctgccgaag gtcgatcgtg cgcccagggc ggcgctggcg ccgatgacac ctccgcgccc
      421 gacggttgcg gtttcgaccg tcgcgccgct ctgcatgacc gccaccatcg agaccatgcc
      481 ggtgtgcggg aaatagacgt gctcgatctc ctctcccacg tcgtgcagca tcacgccgcg
      541 ctggagcggg attgtccgca gatgcggcgc cagcaaagaa aagtcatgcg gcggcaacgc
      601 cgccaggaat cggttgaatt ttccctgctc caaccgccgg gtaacggggg gctggccgta
      661 cggcatcgga gttggctcct gctctaagac ggcgacgccg cgcccattaa gtcagtcgaa
      721 aattgccttt gccgatagcg ccctgcgttc agcgtatcga accgatggat caatgcgtct
      781 ggtcgattca atgagtccgg ctgcaaaaag ttccaaacaa gcccgccgcg atatcgcaaa
      841 gattctatca ctcgcggggc agcaagacca gatgcggggg ggaacggcgg ggtggcttgt
      901 ttgcggcgcg gtaagcgtcg acgtcgtgcg tcggcagggc ggcaaaccgg gcatgcgcca
      961 cgaggtggcg ggctcgaatc tgtatgtttg cgcttacggc caatacggac gaaagctcgt
     1021 gctgcagctg tcgattgcgt tggagcaatt gttcggtctg cttcctcacc gctgacatct
     1081 gctgaacaag cgctgccgat cggcggcctt gccgggtggc aaccgtcgcg gccggctggg
     1141 tcggcttcat ctcggatgat tcggcctgag gctgttggcc ctccgacgtc acgacctcaa
     1201 gggcagggtg cccgatgggc aggcgcagga attcgccgcc ttcgtccacc acctgcaaga
     1261 accagctcgc ccagcgccgc ggattgcccg cgacctcggg atcggccagg tcgcggatga
     1321 ccgccagcgc ctcgttacgg gctgcggagc ggttcggcaa ttcgacgcct tcgtcgtccg
     1381 gcacggtgcg atcgccgaag ctgagatgga agaaatagag cggcatcgac tcttctcccg
     1441 ttttaggaag gaaaccggac cgctatattt cgttccatgg agggtcagca cgcaagctta
     1501 aaaatacgtc gcgctgagtc gccgatcgaa cagacgccga ccgcgatagg catatcatat
     1561 gcatttggga atgctgacaa acagaaggca ggtcgatgcg atcgaaatcc gaccacgcat
     1621 ggcggatggc ggctcaatgc gcaaagctcg cgctgggcgc ggacgagaaa gacgagcggg
     1681 agttctacgt caggatgcgc gactcctgga tcacggtggc aaatcgatac gagttcctcg
     1741 acggcatcga cgagcaaggc gcgccgatga aacggcctcg cagccccgcc gggcaataca
     1801 ttccttatcc cgtaagccgc agcctggaac ggcgacgcgg gccggttgcg cgccgctgat
     1861 ggcagccgct gacacgccgt gatgaccgcg cttgaccgcg gaagatttca cttgcgcgcg
     1921 taacgagaat cttccaaaaa agcgtattca ccaaaatcgc gcttggatgt cggttcggcg
     1981 ccgctgccgg agcggacgca acgctattga ctgactctcg agcttgccga cccgtcgcgg
     2041 acgcgtgtga acgcaacctc cgggtggctg agagccaccg tttcttgttt ccactgttat
     2101 ttttctaccg tcgtttgcag agacaagtgg agccgattgc cttcgcgcac ggcctggggt
     2161 tgtggggcta aagttaaata gctgtggcga caggagaagt cggttgcgat gacacgcgaa
     2221 ctgcggtcca tcgaaggaga gccaagagtt gtgcaatcac agatctcgtt gttcttttat
     2281 tgttatttac aatgaacggc cgcaagcgat cgtgctcgcg ccacagtcca gggttgagca
     2341 ggaacagcgg tacggatgga ttgccgggtc gtaagcgcgt tcacgcacgt cttccaacgc
     2401 gctatgcccg gcaataacgg cacagcagtc ggcgcaacgg acgcgtatca aaccgcctcg
     2461 cgcagcttca ccgcgccttc gaggtcgacc gagacgagtt gcgacacgcc gcgttcggcc
     2521 atggtgacgc cgtagaggcg gttcatgcgc gccatggtga tcggattgtg ggtgatgatc
     2581 acgaagcgcg tg
//
LOCUS       DATLJT010000111         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2072854,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000111 DATLJT010000000
VERSION     DATLJT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072854"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VM910_02455"
     CDS             complement(<1..866)
                     /locus_tag="VM910_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61447.1"
                     /translation="MHLLVGYAAGGPLDTSARLIAQWLSERLGQQFIVVNQPGAGSNL
                     AAETVVRAPPDGYTLLEVSASNAWNATLYDNLKFDFVRDIVPVAGVRRAGGVMEVHPS
                     VPVTTVREFIAYAKANPGKINMATGGVGSAPHLYGELFKTMTGVELVTVNYRGAGPAL
                     LDLISGEVQVTFDVVISSIEHIRAGKLRPLGVTTAKGLTALPDVPPIGDSVPGYDASS
                     WDGIGAPANTPPEIIAILNKEVNAALADPAFKTRLADLGAEPFAGSPAEFGKFVVEYT
                     EKWRKVIRAAGI"
     gene            complement(1580..2350)
                     /locus_tag="VM910_02460"
     CDS             complement(1580..2350)
                     /locus_tag="VM910_02460"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVI61448.1"
                     /translation="MWSAGFRGERELEIYNPAADRWSRGASIPRALHHAAAVGLNGKL
                     FVIGGFVEGWTPTDDVHEYDPASDRWQRLAALPTTRGALAAAVLDGKIHAVGGIGWRG
                     RNTAAHEVYDPAANRWTALPTARDHLAAATIDGRLYAVGGRIDGNYSRNLSSNEAYDL
                     ATNRWEQRAPMPTARSGIAAAVFEGRMFLFGGEEPSGTFNQVQAYDGRSNGWSNHARM
                     PTARHGLGATAVAGRIYVVSGGPTPGDSVSAANKIFVP"
     gene            complement(2313..2507)
                     /locus_tag="VM910_02465"
     CDS             complement(2313..2507)
                     /locus_tag="VM910_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61449.1"
                     /translation="MPRFRTFAAALLVGGALFAAGAFAQGIGRWTTGSPMPSARTEVA
                     VAEVGGKIYVVGGVPRRART"
BASE COUNT          553 a          873 c          804 g          529 t
ORIGIN      
        1 ttaatgccgg ccgcccgaat caccttgcgc cacttctcag tatattcgac gacgaattta
       61 ccgaactcgg caggtgagcc tgcgaagggc tccgcgccca aatcagcgag ccgtgtcttg
      121 aatgcgggat cagcaagcgc ggcgttcacc tccttgttga ggattgcgat gatctccggc
      181 ggcgtgtttg cgggagcgcc gatgccatcc caactgctcg cgtcgtaacc cggcacagaa
      241 tcgccgatcg gcggaacatc cggcaatgcg gtcaggccct tggcggtcgt cacccccagc
      301 ggcctcaact tgcccgccct gatgtgctca atcgacgaaa tcacaacgtc aaatgtgacc
      361 tgcacctcgc cgctaatgag atcgagcagc gcaggccccg cgccgcgata gttcaccgta
      421 accagttcga cgcccgtcat cgtcttgaac aactcgccat aaagatgcgg tgcgcttcct
      481 acgccgcctg tagccatgtt gatctttccg ggattggcct tggcataggc gataaattcg
      541 cgaactgttg taaccggtac cgaaggatga acttccatca cgccgcccgc gcgcctgacg
      601 cctgcaaccg ggacgatgtc gcggacgaaa tcgaatttga gattgtcgta cagtgtggcg
      661 ttccaggcgt tcgacgccga aacttcaagc agggtgtagc cgtctggagg cgcccgcacg
      721 accgtctcgg cggcgagatt gctaccagcg cctggctgat tcacgacgat gaattgctga
      781 ccgaggcgtt ccgacaacca ttgcgcgatc agccgcgcac tggtatcgag tgggccgccc
      841 gctgcatagc cgacgagcaa gtgcactggc cgcgttggat acgtttgagc cccggcgatg
      901 cgcactatgc tcagcggcgc gacggcaccc gctgccaatt gaagaaaagt tcgacggtcg
      961 agtagatgag gaggttgccc atcctcatcc ctcacagacc cggatgtgca gatttcccgc
     1021 ttccggttct tcatgggaga gccttgctcg cggaggtgca gatgacacta tccgtgacct
     1081 gccggcgaca aaagactagt aggacgtccg ccctcgtttc cattcttaac agttcagcta
     1141 ccttcagcgc catgcgatgg gcaacaacag actcttcgag cctcgcccat ctgcggcgca
     1201 tcaagacaat cgtgagggtc gtcacgggca cttgtccgca aaaagcgccc aggggattgc
     1261 gctgattgga ttagcgatcg accagcacgg ctcgtgtttc gacgggccga gttgccactg
     1321 cgcgcttgcc gcagtcgcag tcaacgcaag caacccaaat gcaaagagaa ctttcatcgt
     1381 gacacctctt gttgaagacc ccgagagcat tctcggggac aatcatgaaa tagcggcacg
     1441 gggcgcgatg tctgcgacat ttgtgacact gtaaaagccc tgattttttt acaattcgcg
     1501 cattgtaaaa actgagcgaa atcaaggact gacagcaatt gttctgaatc gagggccgct
     1561 ccacggtgcg agacctgcct cacggcacga aaatcttatt ggcggccgaa accgagtcac
     1621 cgggcgttgg cccgcccgag acgacgtaaa tcctgcccgc gaccgccgtt gcgcccaagc
     1681 cgtgtcgggc cgtcggcata cgcgcatgat tgctccagcc gttgctcctg ccatcgtagg
     1741 cttgcacttg gttgaaggtg cccgacggct cctcgccccc gaacaggaac atccgcccct
     1801 caaacaccgc cgccgcgatg ccgctgcgcg ccgtgggcat cggcgctcgt tgctcccagc
     1861 gattggttgc gaggtcatac gcctcgttcg acgacaaatt gcgcgaatag ttgccatcga
     1921 tgcgcccacc caccgcgtag aggcgtccgt caattgtagc ggccgccaag tgatcgcgcg
     1981 ccgtcgggag cgccgtccac cggttggcag caggatcgta aacctcgtgc gccgcggtgt
     2041 tgcggccgcg ccagccaatg ccgccgaccg cgtggatctt gccatcgagt acggcggccg
     2101 ccagcgcgcc gcgcgttgtg gggagggctg caagtcgttg ccaacgatcg ctcgccgggt
     2161 cgtattcgtg tacgtcgtcg gttggcgtcc agccctcgac aaagccgccg atgacaaaga
     2221 gcttgccgtt taagcccacc gccgctgcgt gatgcagcgc acgtggaatg gaagcgccgc
     2281 ggctccagcg gtcggcggcc ggattgtaga tttcaagttc tcgctcgccg cggaaccccg
     2341 ccgaccacat agattttccc gcccacttcg gccaccgcca cctcggtgcg ggccgagggc
     2401 atcgggctgc cggtggtcca acggccgatg ccttgggcaa aggctccagc agcgaacagg
     2461 gcaccgccga cgagcagcgc tgcagcgaag gttctgaacc taggcatgtc cgttagctcc
     2521 cgccttgatg actattagtg cgttgaaaat tgtttaggcg cagcagaggc gcgccagatg
     2581 cattgcgcta ggctgggcca gctggaactt tgacgttcaa tctttttgac caactcgaac
     2641 agagcttccg tgcattctca cgacgaccga gcagaaccta tgccatggcc tgatgtcgct
     2701 agcgatcaac acagctcgcg accgacatcg tagccataga gccacgcggt ctgaaacac
//
LOCUS       DATLJT010000112         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3221617,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000112 DATLJT010000000
VERSION     DATLJT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221617"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VM910_02470"
     CDS             <1..663
                     /locus_tag="VM910_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI61450.1"
                     /translation="HLLPSPFATLADTWKNIASGAMTRDLLQTLLRVSYAFAIAAAAG
                     VPVGIVLGANENIYRSLEFVIDFFRSTPATAMFPLFLLLFGLGDFSKISVAAFAAWLV
                     IVFNVAYGVMNARQTRILAARSMGASRLRIFKDVIFFETLPQTFVGLRTAVSVALVVI
                     IVAEMFIGAVDGLGHRIIDAQISYQLTDMYGSILVAGAMGYGANLLFLVIEKLFVHWS
                     GR"
     gene            701..1690
                     /locus_tag="VM910_02475"
     CDS             701..1690
                     /locus_tag="VM910_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61451.1"
                     /translation="MKPLKLPALAAALTVLSGGVAAACDRMEKVVAVWLPIMQTTAYY
                     VALEEGLFEKACIEVASNKLEAPNQIIDALVGARADFGPPGAAAGIAMLAESKFPGTF
                     KVFGLQGGGVKVGLINDGLIVKADSDIKSFADLRGKKLGHVPGIQWRTISRHMVRAAG
                     LDPEKDVTLVDLAVGMQVPAVVGGSVDATLSLEPVGSVAVASGQAKRAITNPVAQVIA
                     DPFYSGVSLLTLKFLKERPQVAKKVVEVIDAATKLANDNFDKYKPIIPKYTPIRADQL
                     DLLAKPYLRAFAELNDTDLNSYQALVDVFQKEGVLQSRIDVRDKILKTADFGS"
     gene            1722..2561
                     /locus_tag="VM910_02480"
     CDS             1722..2561
                     /locus_tag="VM910_02480"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61452.1"
                     /translation="MSLAAPQLNVVADAEHPPPAASERPASSRQTFITIRGLSKSFNG
                     TVVYDRFDLDLPQGEFISIFGPNGCGKSTLINMISGLLPLDAGQVLFDGQTIQETRRS
                     YVFQNYRDALFPWLRAIDNIHYPLKLMGLSRRERVARVEQLLADFEVKIDLHAYPYML
                     SGGQQQTVSILRALVTNPEVLFLDEPFSALDYEMTLFMREQLQRVFMKTKTTTLLVSH
                     DLEEAVQLANRVLLLTRRPTRVADAVEIDLPWPRRTESVIDARFVELKSHCLAVFQRE
                     IVH"
     gene            2594..>3264
                     /locus_tag="VM910_02485"
     CDS             2594..>3264
                     /locus_tag="VM910_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HVI61453.1"
                     /translation="MKKKRFRAAAAQTLARLGDVQYNIEISTQLVNEAVRQGAELVVL
                     PECMNTGYLFDSADHCRALAETVPDGAFVSAMSSLARKHDIFIASGITEWDAARGRLF
                     NSGIMLDRKGEVAVHYHKQFLATHDQNWFSFGERGCPVVDTDLGRIGLMICFDGRIPE
                     IARSLALQGAEIIVDMANFFAMDQADMWGPARSYENGVWLVAATKAGYERSIYYPGGS
                     MIVDP"
BASE COUNT          594 a         1048 c          977 g          645 t
ORIGIN      
        1 catttgttgc catcgccatt cgccacgctc gcggacacct ggaagaacat cgcgtcgggc
       61 gcgatgacgc gcgacctgct gcagacgctg ttgcgcgtga gttatgcctt tgccattgcc
      121 gccgcggccg gcgtgcccgt cggcatcgtg ttgggcgcca atgaaaacat ctatcgctcg
      181 ctggaattcg tgatcgactt ctttcgatcg acgcctgcga ccgcgatgtt cccgctgttt
      241 ctcctcctgt tcggtctcgg cgacttctcc aaaatatcgg ttgcggcctt cgcggcctgg
      301 ctcgtgatcg tgttcaacgt cgcctatggg gtgatgaacg cgcgccagac gcgcatcctg
      361 gccgcgcgct ccatgggggc gagccggttg cgcatcttca aagacgtcat cttcttcgag
      421 acgctgccgc aaacgttcgt gggtctgcgc accgccgtct cggtcgccct ggtggtgatc
      481 atcgtcgccg agatgttcat cggggcggtc gatggtctgg gtcatcggat catcgatgcc
      541 cagatctcct atcagctcac cgacatgtac ggttcgatcc tggtcgccgg ggcgatgggc
      601 tacggcgcca atcttctgtt cctcgtgatc gagaagctgt tcgtccactg gtccggacga
      661 tgacttttgg tcccatctca accgcagagg gagacgcgcg atgaaacctc tcaagcttcc
      721 ggcgctggcg gccgccttga ccgtgctttc gggtggcgtc gccgcggcct gcgaccgcat
      781 ggagaaagtc gtggcggtgt ggctgccgat catgcagacc acggcctatt acgtcgcgct
      841 tgaggagggg ctgttcgaaa aggcctgcat cgaggtcgcc tcgaacaaat tggaagctcc
      901 caaccagatc atcgacgcgc tagtgggcgc ccgcgccgat ttcggccctc ccggcgcggc
      961 ggcaggcatc gcgatgctcg cggagtcgaa gtttcccgga accttcaagg tgttcgggct
     1021 gcaaggggga ggggtcaagg tcggcttgat caacgacggc ctcatcgtca aggccgacag
     1081 cgacatcaaa agcttcgccg acctcagggg caagaagctt ggtcatgtgc cgggaattca
     1141 atggcgcacg atttcccgcc acatggtgcg cgcggccggt ctcgatccgg aaaaggacgt
     1201 gacgctcgtc gacctcgccg tcggcatgca ggttcccgcc gtggtcggcg gcagcgtcga
     1261 cgccacgctc tcgctcgaac ccgtcggttc cgtcgccgtg gcgtcgggcc aggccaagcg
     1321 ggccatcacc aatccggtgg cgcaagtcat cgccgatccg ttctattccg gcgtgtcgct
     1381 gctgaccctg aagttcctca aggagcgacc acaggttgcc aagaaagtgg tcgaggtcat
     1441 cgacgccgcc accaaactgg ccaatgacaa cttcgacaag tacaagccga ttattccgaa
     1501 atacacgccg atccgcgccg atcagctcga tctcttggcc aagccctacc tgcgcgcatt
     1561 cgccgagctc aacgacaccg acctcaactc ctatcaagcg ctggtcgacg tctttcaaaa
     1621 ggaaggcgtg ctgcaatcgc ggatcgacgt gcgggacaag atcctcaaga cggccgactt
     1681 cggctcgtga tggcgcggtg tttccaccca aggcccacga tatgtcactg gccgcaccac
     1741 aattgaacgt tgtcgcggac gcggagcatc cgccgccggc ggcgagcgag cgacctgcgt
     1801 cttcccgcca gaccttcatc accattcgcg gcctgagcaa gtcgttcaac ggtaccgtcg
     1861 tctacgaccg cttcgacctc gatctgccgc aaggggaatt catctcgatc ttcgggccga
     1921 acggctgtgg caagagcacc ttgatcaaca tgatctccgg gctgctgccg ctggatgccg
     1981 ggcaggtgct gttcgacggg cagaccattc aggagacgcg gcgctcctac gttttccaga
     2041 actatcgcga cgccctcttc ccgtggctgc gcgccatcga caacatccac tatccgttga
     2101 agctcatggg gctgtcgcga cgcgaacgcg tggcgcgggt cgagcagctg ctcgcggatt
     2161 ttgaggtcaa aatcgatctc catgcgtacc cctacatgct gtccggcggc cagcagcaga
     2221 ccgtttcgat cctgcgcgcg ctcgtcacca atccagaagt gctctttctc gacgagccgt
     2281 tttcggcgct cgattacgag atgaccctgt tcatgcgcga gcagctgcaa cgggtcttta
     2341 tgaagacgaa aacaacgacg ttgctggtct cgcacgatct cgaagaggcg gtgcagctgg
     2401 ccaaccgcgt cttgctgctg acccgtcggc cgacgcgagt ggccgatgcc gtggagatcg
     2461 atctgccatg gcctcgccgc accgaaagcg tgatcgacgc gcgcttcgtc gagctcaaga
     2521 gccactgcct cgccgtcttc caacgtgaaa tcgtccactg acggccgccg aatatcctac
     2581 ggggagaaat acgatgaaga agaaacgttt ccgcgccgct gccgcccaga cgctcgcccg
     2641 gctcggcgat gtccaataca atatcgaaat ctccacgcaa ctggtgaacg aggcggtgcg
     2701 acagggcgcc gagctcgtgg tgctgccgga gtgcatgaac accggctatc tgttcgattc
     2761 ggccgaccat tgccgcgcgc tcgccgagac cgtgcccgat ggcgccttcg tgtccgcaat
     2821 gtcctcgctt gcgcgcaagc acgacatttt catcgccagc gggatcaccg aatgggacgc
     2881 cgcccgcgga cgactgttca attccggaat tatgctcgac cgcaagggcg aagtggccgt
     2941 tcactaccac aagcaatttc tcgccaccca tgatcaaaac tggttttcct tcggcgagcg
     3001 cggatgcccg gtcgtcgaca ccgatctcgg ccggatcggg ctcatgatct gcttcgacgg
     3061 gcgcattccg gaaatcgcgc gcagcctcgc gctgcaaggt gccgagatca tcgtcgatat
     3121 ggcgaacttt ttcgccatgg atcaggccga catgtggggt ccggcgcgct cttatgagaa
     3181 cggcgtctgg ctggtggccg cgaccaaggc cggctacgag cgatcgatct actaccccgg
     3241 cggcagcatg atcgtcgatc cgaa
//
LOCUS       DATLJT010000113         4446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3911051,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000113 DATLJT010000000
VERSION     DATLJT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911051"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VM910_02490"
     CDS             <1..559
                     /locus_tag="VM910_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61454.1"
                     /translation="LRAAFGIGFCILFAFIGTFTYVNFVLVRPPLSLGPMELGFVYFV
                     FLPSVVTTLWAGRAVHRYGTRPTLWGSLALAGVGLPLLLLPNLAAVLVGMVLVGVGTF
                     FAQATATGFVSRAATADRGAASGIYLACYFAGGLVGTAILGQIFDRIGWPACVAGIGL
                     ALGAAAVLAHWLKLQADPAPAMRTA"
     gene            complement(583..1512)
                     /locus_tag="VM910_02495"
     CDS             complement(583..1512)
                     /locus_tag="VM910_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI61455.1"
                     /translation="MHQVRYFLTLCQELNFTRAAEKCNVAQPALTRAIKLLEHEFGGL
                     LFHRERARTHLSELGQAVRPYLEEVQLQSQQAKRLATSFIELKGTPLKLGIMCTIGPS
                     NFVGLLTNLQAQYPGIELQIMDAGASDLQGLLLEGGLEAAIYCWPDQIDERLHYLPLF
                     REQFFIVLGRQHRLANRPEVRVGDLNGERYLNRINCEQVNLARDIFAQRGTKVERVYR
                     SDRDDWILAMVAAGLGFAFMPQYSITERPDVVVRPLVEPEFWREVSLVTVRGRPHSPA
                     IGALVREAMRTQWLGADALAVQKSRARDGGKMH"
     gene            1690..2490
                     /locus_tag="VM910_02500"
     CDS             1690..2490
                     /locus_tag="VM910_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVI61456.1"
                     /translation="MAPARMVECRGRKRYRKLIAMSQLVVASNNPALSCPLRAHVAHD
                     LRNLLATVGLHLETLQRLSGPSGAKAANAAHALLTRGAALCNDALDRTAGADSRARRR
                     GVDMIGIARQVADLLAPAAPKDFSFDINQSCSASVVADSNETFRILFNLMSNAVAVAN
                     RKPASLTSVTLHVSTEDSVTIIRLADDGPGLPAGVRTRLFRARSRRSAACRHGYGLTI
                     ARELAERNGGTLTLVPSAKGTTFALKFPAFVSVVRDRPQDLGRRAMAV"
     gene            2901..3257
                     /locus_tag="VM910_02505"
     CDS             2901..3257
                     /locus_tag="VM910_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61457.1"
                     /translation="MQMKRIYQVQHWRYGQWIREHNWCKVEAFSEKEAAEKVCGVALT
                     EAGKLAQLRARVLTLGDRKQTRATSFYAVELATDPAPVSSQCNVRSMSVGRSIQGKRS
                     AGPMGPSATGCGLLLP"
     gene            3254..>4446
                     /locus_tag="VM910_02510"
     CDS             3254..>4446
                     /locus_tag="VM910_02510"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HVI61458.1"
                     /translation="MRFAALAASLLGTIVFGALIAYGAGFIPPFALCGILSAIGIWDL
                     LQSPHSTRRNYPILANIRFMLEKIRPEIRQYFLESETDGVPFNRSKRAIAYQRAKGAL
                     DKRPFGTQQDIYGDNFEWINHSITPHPIAGHDFRVTVGGADCRQPYALSLFNISAMSF
                     GALSANAIRALNRGAKLGGFAHDTGEGGISRYHREFGGDLIWEIGSGYFGCRNEDGSF
                     CASRFATQAQEPQVKMIEIKLSQGAKPGHGGVLPGAKVTAEIADARGVPVGVDCISPA
                     RHSAFNTPVELMQFIARLRHLSGGKPVGFKLCIGHAWEFMAICKAMLETGIHPDFIVI
                     DGSEGGTGAAPLEFVDHVGTPLREGLVLAHNCLVGAGLRQSVRLGASGRVVTAFDIAR
                     LLALGA"
BASE COUNT          792 a         1398 c         1386 g          870 t
ORIGIN      
        1 actgcgcgcg gccttcggca tcggattttg catcctgttc gcgttcatcg gcacgttcac
       61 ttacgtcaac ttcgtcctgg tgcgtcctcc gctgtcgctc ggccccatgg agctcggctt
      121 cgtctatttc gtgttcctgc catcggtggt gacgacgcta tgggcggggc gcgccgttca
      181 ccgctacggg acgcggccga cgctatgggg ctcgctcgcg ctggcgggcg tcggcttgcc
      241 gctgcttctg ctaccgaacc tggcggcggt tttggtcggc atggttctcg tcggcgtggg
      301 gaccttcttc gcccaggcga cggcgaccgg cttcgtcagc cgcgctgcga ccgcggaccg
      361 cggggcagcg agcggcatct atctcgcctg ctacttcgcc ggcggcctgg tcggcaccgc
      421 gatcctgggg caaatcttcg accggatcgg atggccggcc tgcgtcgccg gaattggcct
      481 agcgctcggc gccgccgcgg tgctcgcgca ttggctgaaa ctgcaggccg atcccgcccc
      541 cgcgatgcga accgcatgag gtcactgcgg gtgatcggcc ggtcaatgca tcttgccgcc
      601 gtcgcgcgcg cggctctttt gtaccgccag cgcatccgca cccagccact gcgtgcgcat
      661 ggcttcgcgc acgagcgcgc cgatcgccgg cgaatgcgga cgtccgcgca cggtcaccag
      721 gctcacctcg cgccaaaatt cgggctcgac caatggccgc accacgacat ccgggcgctc
      781 cgtgatggaa tattgcggca tgaaggcaaa gccgaggccg gctgcgacca tggcaagaat
      841 ccaatcgtcg cggtcgctac gatacacccg ttcgaccttc gtgccgcgct gggcaaaaat
      901 atcgcgcgca aggttcactt gctcgcaatt gatgcggttg aggtaacgct cgccgttcag
      961 atcaccgacg cggacctcgg gccggttggc caaccgatgc tggcgaccaa gcacgatgaa
     1021 gaattgctca cggaagagtg gaagataatg aagccgctcg tcgatctggt ccggccagca
     1081 atagatggcg gcctccagtc cgccttcgag caggaggccc tgaaggtcgg aagcgcccgc
     1141 gtccatgatc tgcagctcga tgccggggta ttgcgcctgc aagttcgtca ggagtccgac
     1201 gaaattgctg ggaccgatcg tgcacatgat cccgagtttt aacggcgtgc ccttgagctc
     1261 gatgaaactc gtggcaagcc gcttcgcctg ctgcgattgc agctgaacct cttcgagata
     1321 gggacgcacc gcctggccaa gctcgctcag atgggttcgc gcccgctcgc gatggaacag
     1381 gagcccgccg aactcgtgct ccagcaactt gatggcacgc gtgagcgccg gctgcgccac
     1441 gttgcatttc tcggccgccc gggtgaagtt caactcctgg caaagggtta gaaagtacct
     1501 gacctgatgc atttccacgg cgacaacctc tcagtgcacc gtcacgccac gcttcggcct
     1561 gctcccgcag cggccgccgt tggaataacc gcgcggtttc atttcggcaa gtggccgatg
     1621 gttagccact cattgcgagt cactacaatg tccccgcgct atcgatccca taggcgatcg
     1681 gtattggctt tggcgcccgc ccgcatggtc gaatgcagag gccgaaagag gtatcggaag
     1741 ttaattgcca tgtcgcaact cgtcgtcgcc tcgaacaatc cggcactaag ctgtcccctt
     1801 cgcgcccacg tggcccacga cttgcgcaac ctccttgcca ccgtggggct ccatctcgaa
     1861 acgctgcaac gtctgtccgg cccgagcggt gccaaagcgg ctaatgctgc gcacgccctg
     1921 ctgacacggg gcgccgcctt gtgcaacgac gccctggatc gtaccgcggg tgcggatagc
     1981 cgcgcgcgcc ggcgaggcgt agatatgatc gggatcgcac gccaggtcgc tgatctactg
     2041 gctcccgccg cgcccaagga tttttccttt gatatcaacc agagttgtag cgcctccgtt
     2101 gtcgccgatt cgaacgaaac gttccggatc cttttcaacc tgatgagcaa tgccgtcgcg
     2161 gtcgcgaacc gcaagccggc ctcgctcaca tcggtcacac tccatgtcag caccgaagac
     2221 tcggtgacga tcatacgcct cgccgatgac gggcccgggc tgccggccgg cgtccggacg
     2281 agattgttca gggcgcgatc gcgccgatcg gccgcatgtc gtcatggcta cggccttaca
     2341 atcgcgcgcg aacttgccga gcgtaacggc ggcacgctta ccctcgtccc gtcggccaag
     2401 ggcacgacat tcgcactgaa gtttccggcc tttgtctccg tggtgcgaga cagaccgcag
     2461 gacctgggcc gtcgagctat ggccgtgtga ctcaaccgtg gagccgccgg gaaattatgg
     2521 tccgctttgg cacgagcgag atttgatggc tcgtcacttg ccaactgcac gttacgttca
     2581 cgactcgctg acggacctcc cctaccggag tgtgaccagt gggcatgctc tccgcatgat
     2641 ggggaggggc ttctgcccgc tgccggccat acggtggtcc cgggtgttgc cactccagca
     2701 atgcctcaga gctatcgaca ttatcaagaa acggcatttc agttcccgtg tcctgtggag
     2761 cttcatagtg ctcgctaccc cggcccgctg tctaactgac ggcgcggagc tccgcccggc
     2821 ataaatcccg ctcggatgtc tccggtagcc aaaggctcgg ggcgccatat cgcctgcccc
     2881 acgaacgtcg ggagaaagcg atgcagatga agcggattta tcaggtccaa cattggcgct
     2941 atgggcaatg gatccgcgag cacaactggt gcaaagtcga ggcattcagc gaaaaagaag
     3001 ctgcggagaa agtgtgcggc gttgcgctga ccgaagcggg caagctcgcg cagctccgcg
     3061 cccgcgtcct caccctgggc gatcgcaagc aaactagagc gacgtcgttt tatgcagtcg
     3121 agctggccac cgaccccgca ccagtgagtt cacagtgcaa cgttcgctcg atgagtgtag
     3181 gtcgatcaat ccaggggaag cggagcgcag gccccatggg tccttcagct accggctgcg
     3241 gtcttttgct gccatgagat tcgcagcact agcggcaagt ttgctcggaa ccatcgtttt
     3301 cggagcgctg attgcttacg gtgccgggtt catcccgccg ttcgcgctgt gcggaatatt
     3361 gagtgcgatt ggtatttggg acctgttgca atctccgcac agcacccgac gaaactatcc
     3421 catcctcgcc aatatccgct ttatgctgga gaagatcaga ccggagatcc gtcaatattt
     3481 cctggaaagc gagaccgacg gcgtgccgtt caaccgatcg aaacgcgcga tagcgtacca
     3541 gcgcgcgaag ggagcgctcg acaagcggcc gttcgggacg cagcaggaca tctacggcga
     3601 caatttcgaa tggatcaatc attcgattac tccccatccg attgcaggcc acgacttccg
     3661 cgtcacggtg gggggcgccg actgccggca gccctatgcc ctgtcgctct tcaacatctc
     3721 agccatgagc ttcggcgcgc tgagcgcgaa tgcaattcgc gccctgaaca ggggtgccaa
     3781 gctcgggggt ttcgcgcatg acaccggcga gggcgggatc agccgatatc atcgcgagtt
     3841 tggcggcgac ctgatctggg agatcggtag cggctatttc ggctgccgca atgaggatgg
     3901 ctcgttctgc gccagccggt ttgccacgca ggcacaagag ccgcaggtca agatgatcga
     3961 gattaagctc tcgcaaggag cgaagccggg ccatgggggc gtgttgcctg gcgccaaggt
     4021 gacggccgag atcgcagatg cccggggcgt gccggtcggc gttgattgta tctcaccggc
     4081 tcgccactcg gcattcaata cgccggtcga gttgatgcag ttcatcgcgc gattgcgcca
     4141 tttgtcgggc ggcaagccgg tcggcttcaa gctctgcatc gggcacgcct gggagtttat
     4201 ggcgatctgc aaagccatgc tcgagactgg aatccatcct gatttcattg tcatcgatgg
     4261 ctcggagggt ggaaccggag cagcgccgct cgaattcgtg gatcacgtcg gcacgccgct
     4321 gcgagaaggt ttggtgctcg cgcacaattg tctggtggga gcgggattgc ggcaatccgt
     4381 tcgactgggg gccagcgggc gggtggtaac cgcctttgat atcgcgcgtc tgctggcgct
     4441 tggggc
//
LOCUS       DATLJT010000114         5050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1154181,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000114 DATLJT010000000
VERSION     DATLJT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154181"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VM910_02515"
     CDS             <1..286
                     /locus_tag="VM910_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ligand-gated channel"
                     /protein_id="HVI61459.1"
                     /translation="YLWNDTRFSASMIYGSGLRSGFANTDHLPSYNQVNMGVSHDFYI
                     VAPNKPTTLRFDVVNVFDKIYEIRDGSGIGVFAPQFGPRRAFYFGISQKI"
     gene            415..1143
                     /gene="exbB"
                     /locus_tag="VM910_02520"
     CDS             415..1143
                     /gene="exbB"
                     /locus_tag="VM910_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HVI61460.1"
                     /translation="MLAHPALAQAPNPLLPRNLSPWGMFLNADIVVKVVMVGLAFASL
                     VTWTVWLAKTIELRVATRLARRRMKMLEEGGSLSAAVRACGDERDAIAQLILSTAREA
                     ELSDGIVDEGFKERVALRLERVEAAMTRQVSRGVGILATIGATAPFVGLFGTVWGIMN
                     SFIGISEAHTTNLAVVAPGIAEALLATALGLVAAIPAVVIYNHLARVIAGYRTLLSDA
                     SAQLLLLISRGHRGRAATLPRAAE"
     gene            1149..1580
                     /gene="exbD"
                     /locus_tag="VM910_02525"
     CDS             1149..1580
                     /gene="exbD"
                     /locus_tag="VM910_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HVI61461.1"
                     /translation="MALRLGSRSPDDLEIQHEINVTPFIDVILVLLIIFMVAAPLATV
                     DIGVNLPASTAPEQPRPDKPIFVTIKPDLSIAIGETMVSREGLPEALDLATNSHKDET
                     IFIRADKALSYGELMEVMNTLRDAGYLKLALVGLETPAEKK"
     gene            1577..2299
                     /locus_tag="VM910_02530"
     CDS             1577..2299
                     /locus_tag="VM910_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVI61462.1"
                     /translation="MNALALHLPEERSLSRWMLAGLAILLAHAAIVAAVVLWYARQPV
                     QPNIIPAIAVSLAPVEASSPEIQNQDIAIGPAMQQAEEVPKEQPKEEKPTEKLQEPPP
                     PQPQAEVTLPREEPKQVEKPKAAVQPPAPETRAPPKTEHVGQFTVAAANAYNALVFGH
                     LQRFKRYPLAAKGASGTVVLRFDLSRAGEVINSTVTKSSGNALLDEAALDLLRRASPF
                     PPFPALKPGSRDSYIAPVNFSR"
     gene            complement(2420..2839)
                     /locus_tag="VM910_02535"
     CDS             complement(2420..2839)
                     /locus_tag="VM910_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61463.1"
                     /translation="MKRLSLIAIAAALFMALPTPAVLAQSTQDTGATPPPPPPPPPPP
                     PAPPPPAAAPAPAPPPAAAAPTTTAPQTTTTTTDKPATEKPTTTTEKETGTKKKKSGK
                     STSRQQEIDHSIQSGTVPSRYRSQVPKEYQKYIPFEK"
     gene            complement(2997..4433)
                     /locus_tag="VM910_02540"
     CDS             complement(2997..4433)
                     /locus_tag="VM910_02540"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVI61464.1"
                     /translation="MSFVARHALWSEEQKEAARRLRRLAEEKNLETIRLSFADQHGIL
                     RGKTLVASEALASLESGCTITTTMLAKDTAHRTVFPVFTAGGGFGMKEMEGAADVLMV
                     PDPTTFRVLPWSPATGWVLCDIYFGDGRPVPFSTRALYRSVLNKLAERGYDFVVGLEV
                     EFHIFKLDDPKMAPEDAGQPGQPPSVSLLSHGYQYLTEQRYDQMEPALDIIRRDITAL
                     GLPLRSVEVEFGPSQCEFTFAPRRGLEPADNMMLFRSAVKQIARRHGYHATFMCRPKL
                     PNAFASGWHLHQSLASRTTGENVFAAKEGSTPLSDHGRCYLAGLLEHARASTVFTTPT
                     INGYKRYRSYSLAPDRAIWGKDNRGVMIRVLGGAGDGATRLENRIGEPAANPYLYMAS
                     QIVSGLDGIDRKRDPGPSADTPYETKADLLPKSLREAVTALNDDPFFRQALGAEFVDY
                     YVHIKNAEIERFQAEVSDWEHREYFEMF"
     gene            complement(4456..>5050)
                     /locus_tag="VM910_02545"
     CDS             complement(4456..>5050)
                     /locus_tag="VM910_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVI61465.1"
                     /translation="HAFVIPMNTEMTITQYHVPIDDENCYWYSIFTSYSTPVDKKKMR
                     DQRLATYELPDYKSRRNKANDYGFDPHEQETATYTGMGMDINVHDQWAVESMGAIQDR
                     TKEHLGQSDKAIVQYRRMLRDQIEKVASGEKPILCLDQDNARSIQGPATMDGIGPTQG
                     WEIYWMEVDVRRRRGAPWAAPVPQDIAGKVTHLTAAE"
BASE COUNT          866 a         1569 c         1653 g          962 t
ORIGIN      
        1 gtatctgtgg aacgacacgc gcttttccgc ctcgatgatc tatggcagcg ggcttcgttc
       61 gggctttgcc aacaccgatc acctgccgtc ctacaaccag gtcaatatgg gcgtgtcgca
      121 cgatttctac atcgtggcgc caaacaagcc gaccacgctg cgcttcgatg tcgtgaacgt
      181 gttcgacaag atctatgaga tcagggacgg ctccggtatc ggtgtgttcg ccccgcaatt
      241 cgggccacgc cgggccttct atttcggcat ttcgcagaaa atatgagcgt ggaagcaatg
      301 atgggccaaa cagaagaaaa gatgcggcac tgcggaaaga caggcggcga ccggctttgg
      361 cgcgcgccga taaggcggat cgcggggatc gcgggccttg cgttcgcggc tgccatgctc
      421 gcgcatcccg ctctggcgca ggcgccaaat cccctgcttc cgcgcaacct ctcgccatgg
      481 ggcatgttcc tcaacgccga catcgtggtg aaggtggtca tggtcgggct ggcctttgcc
      541 tcgctcgtga cctggacggt ctggctcgcc aagacaatcg aactcagggt cgcgaccagg
      601 ctggcgcggc ggcggatgaa aatgctggag gagggcggca gcctttcggc ggcggtgcgc
      661 gcctgcggcg acgagcgcga tgcgattgcg cagctcatcc tgtccaccgc gcgggaggcg
      721 gaactctccg acggcatcgt cgatgagggc ttcaaagagc gcgtggcctt gcggctcgag
      781 cgcgtggagg cggcgatgac gcggcaggtc tcgcgcggcg tcggcattct ggccaccatc
      841 ggcgccacgg cgccgttcgt cggcctgttc ggcacggtgt gggggatcat gaactccttc
      901 atcggcattt cggaagccca caccaccaat ctcgcggtgg tggcgccggg catcgcggaa
      961 gcgctgcttg cgaccgcgct tggcctggtc gccgccattc cggcggtcgt gatctacaac
     1021 catcttgccc gcgtcatcgc cggctaccgc acgctgctca gtgacgcctc ggcacagttg
     1081 ctgctgttga tcagccgcgg gcatcgcggt cgcgccgcga cgctgccccg cgcggcggag
     1141 taaccgacat ggcgctccgt ctcggttcac gctcgcccga tgatctcgag atccagcacg
     1201 agatcaacgt cacgccgttc atcgacgtga tactggtgct gctcatcatc ttcatggtcg
     1261 ccgcgccgct cgcgaccgtc gatatcggcg tcaatcttcc cgcctcgacc gcgcccgaac
     1321 agccgcggcc ggacaagccg atcttcgtca ccatcaagcc ggatctttcg atcgcaatcg
     1381 gcgagaccat ggtttcgcgc gaaggactcc cggaggcgct cgatctggcc accaacagcc
     1441 acaaggatga gacgatcttc attcgcgccg acaaggcgct gagctatggc gaactgatgg
     1501 aggtgatgaa caccttgcgc gatgccggat atctcaagct tgccctggtc gggctcgaaa
     1561 cgccggccga gaagaaatga acgccctcgc gctgcatctg ccggaagagc gctcgctgtc
     1621 gcgctggatg ctcgcgggcc tggcgattct tctcgcccat gctgcgatcg tggcagcggt
     1681 tgtgctgtgg tatgcaagac agcccgtcca gccgaacatc attcccgcga ttgccgtgag
     1741 tctggcgccg gtcgaagcca gttcgccaga gattcaaaat caggacatcg cgattggacc
     1801 ggccatgcaa caggccgagg aagtaccgaa ggagcagccg aaagaagaga agccgacgga
     1861 aaagctgcag gagccgccac cgccccagcc gcaagccgag gtcacgctgc cgcgggaaga
     1921 accgaagcag gtggagaagc cgaaggccgc ggttcagccg cctgcgccgg agacgcgtgc
     1981 gccgccgaag accgagcatg tcgggcagtt caccgttgcc gccgccaacg cctataacgc
     2041 gctcgtgttc ggccatctgc agcgcttcaa gcgctatccg ctggccgcga agggggcatc
     2101 gggcacggtg gtgctgcggt tcgacttgag tcgcgcgggt gaggtgatca acagcacggt
     2161 cacgaaatct tccggcaacg cgctgctgga tgaggcggcg ctcgacctgc tccggcgcgc
     2221 cagtccgttc ccgccgtttc ctgccttgaa gcccggcagc cgggacagct acatcgcgcc
     2281 cgtgaatttc tcgcggtagc gcatgatccg gaaaggcgcg cagcggcgcg caccgtgaga
     2341 tcaatcactc aggacaagat ccggacaacg cgatcgcatc ctcggacaag aatgcgatcg
     2401 cgcagaacat cacgcgccgt tatttctcaa acggaatgta tttctggtat tccttcggca
     2461 cctggctgcg atagcgcgac ggcaccgttc cgctctggat cgaatggtcg atctcctgtt
     2521 gccggctcgt cgacttgccc gactttttct tcttcgttcc cgtctccttc tcggtcgtgg
     2581 tggttggctt ttcggttgcc ggcttgtcgg tggtcgtggt ggtcgtctgg ggcgccgtcg
     2641 tggtcggggc tgctgctgcg ggcggcggag ccggtgcggg cgctgcggcc ggaggcggag
     2701 gagccggcgg gggcggaggg ggtggtggag gcggcggagg cgtcgcgccg gtatcctgcg
     2761 tcgattgggc cagcacggcc ggcgtcggca gggccatgaa cagagctgcg gctatcgcaa
     2821 taagagaaag tctcttcatc ggaaaactcc gggtgttgat cccgctggcg gaacaataca
     2881 gtagcgcaag catacaccaa ttttgaccgg ggtctgcccg ccgtcggtcg gagcccgctt
     2941 cgaggcggga gggggtcaat gcgcccggcc cttcgcggct tcgggcggtg aacgggtcaa
     3001 aacatctcga aatattcgcg gtgttcccag tccgacactt cggcctggaa gcgttcgatc
     3061 tcggcattct tgatgtgcac gtaatagtcc acaaactccg cgccgagtgc ctgccggaag
     3121 aagggatcgt cattcagcgc tgtgaccgct tcgcgcagcg atttcggcaa gaggtcggcc
     3181 ttcgtctcat agggcgtatc cgccgagggg ccggggtcgc gcttgcggtc gattccatcg
     3241 aggcccgaga caatttgcga ggccatgtag agatagggat tggcggcggg ctcgccgatc
     3301 cggttctcaa gccgcgttgc gccgtcgccc gcgccgccga gcacgcggat catcacgccg
     3361 cgattgtcct tgccccagat cgcgcgatcc ggcgccagcg aataggagcg gtagcgcttg
     3421 taaccgttga tggtcggcgt ggtgaacacg gtcgatgcac gggcgtgttc cagcaggcct
     3481 gcgagatagc agcggccatg atcggaaagc ggcgtggagc cctccttcgc cgcgaacaca
     3541 ttctcgccgg tcgtgcgcga cgccagcgac tggtgcaggt gccatccgct tgcgaatgca
     3601 ttcggaagtt tcggccggca catgaaggtc gcgtggtatc catgccgccg cgcaatctgt
     3661 ttgaccgcgc ttcggaacag catcatgttg tccgcgggct cgagaccccg gcgtggcgca
     3721 aaggtgaatt cgcactggct cgggccgaac tcgacttcga ccgaacgcaa gggcaggccg
     3781 agtgccgtga tgtcgcgcct gatgatgtcg agcgcgggct ccatctggtc gtagcgctgt
     3841 tcggtcagat attggtagcc gtgcgagagc aggctgacgg acggcggctg gccgggttgg
     3901 cccgcatcct ccggcgccat tttgggatcg tcgagcttga agatatggaa ttcgacttca
     3961 aggccgacga cgaaatcgta gccgcgttcg gcaagcttgt tgaggaccga acgataaagt
     4021 gcgcgcgtcg agaagggtac cggtcggccg tcgccaaaat aaatgtcgca caacacccag
     4081 cccgtggccg gcgaccaggg cagcacgcgg aaggtcgtgg gatcgggcac catcaagacg
     4141 tcggcagcac cttccatctc cttcatgccg aaaccgccgc cggcggtgaa taccgggaac
     4201 accgtgcggt gcgcggtgtc tttggcgagc atcgttgtgg tgatggtgca tccgctttcg
     4261 agcgaggcaa gcgcctccga tgccaccagc gtcttgccgc gcagaatgcc gtgctggtcg
     4321 gcaaaggaca ggcggatggt ctcgaggttt ttttcttcgg caaggcggcg cagccgccgc
     4381 gccgcttcct tctgctcctc ggaccaaagc gcgtgccgcg cgacgaaact caacgcatgt
     4441 ccccccgcaa gccaatcatt cagccgccgt cagatgcgtc acttttccgg cgatgtcctg
     4501 cggcactggt gccgcccatg gcgcaccacg ccgccgcctc acgtcgactt ccatccagta
     4561 gatctcccag ccctgggtcg gcccgatgcc gtccatggtg gccgggccct ggatactgcg
     4621 ggcgttgtcc tggtccaggc acaggatcgg cttctcgccg gaagcgacct tctcgatctg
     4681 gtcgcgcagc atgcggcggt attgcacgat cgccttgtcg ctctggccga gatgttcctt
     4741 ggtgcgatcc tggatcgcgc ccatcgattc caccgcccac tggtcgtgca cgttgatgtc
     4801 catgcccatg ccggtatagg tcgcggtttc ctgctcgtgc ggatcgaagc cgtaatcgtt
     4861 cgctttgttg cgtcgcgact tgtagtcggg cagctcgtac gtcgccagac gctggtcgcg
     4921 catcttcttc ttgtcgaccg gcgtcgagta ggaggtgaag atcgagtacc agtagcagtt
     4981 ctcgtcgtcg atcggcacgt ggtactgcgt gatcgtcatc tcggtgttca tggggatgac
     5041 aaaagcgtgc
//
LOCUS       DATLJT010000115         1939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3221834,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000115 DATLJT010000000
VERSION     DATLJT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221834"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..846)
                     /gene="otsB"
                     /locus_tag="VM910_02550"
     CDS             complement(82..846)
                     /gene="otsB"
                     /locus_tag="VM910_02550"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVI61466.1"
                     /translation="MSNSIPSLPADLKQFAFLLDVDGTILDLAPTPREVFVSHELRDT
                     LARLWKRTDGGVAFVSGRPVSELDLIFSPLQLPAIGGHGAELRTTPGAAPEAPRLPAL
                     DPAVKRQFAAIAEAGPGIILEDKGYSLALHYRLAPDKEELVRAAAAKICAGVKGASIE
                     LLPGKLVVEIKQTGFTKATAVRELMSYPRFSHRRPIFLGDDVTDLGVFEILPDFDGIG
                     ISVGRNVPGVEARFAQPADVRRWLEQISRNDAFATS"
     gene            complement(1079..1837)
                     /locus_tag="VM910_02555"
     CDS             complement(1079..1837)
                     /locus_tag="VM910_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI61467.1"
                     /translation="MALRLQGKLALITAAGQGIGRAIAEAFAVEGARVIATDLEEKKL
                     EGIKSVKGRKLDVRSTAAIEALAKETATEFGALDVLVNCAGYVHHGSVLDCSEQDWDF
                     SFDLNVKSMHRMIKAFLPGMLQKKAGSIVNISSAVSSIRGVPDRYAYGATKAAVIGLT
                     KAVAADFIRQGIRANAICPGTIESPSLEDRIKDRSRATGKSLAEVEKAFVERQPLGRL
                     GRPEEVAALAVFLASDEASYITGQPHLVDGGMAL"
BASE COUNT          363 a          590 c          635 g          351 t
ORIGIN      
        1 accacaccag acgcgcggtg ggatcgacga gcgcggccgt gcgtccgttg ccgatgacgg
       61 caagatcaag cccgtggtcg gtcatgatgt cgcgaatgcg tcgttgcgag agatttgctc
      121 gagccagcgg cgaacatccg cgggctgggc gaagcgagcc tcgacgccgg ggacgttgcg
      181 gccgactgaa ataccaatgc catcgaaatc gggcagaatc tcgaacacgc ccagatccgt
      241 cacgtcatcg ccgaggaaaa tgggacgccg gtgggagaag cgcggatagc tcatcagttc
      301 gcgaacggcg gtcgccttgg tgaaaccggt ttgcttgatt tccaccacca attttccggg
      361 caagagctcg atcgaggctc ccttcacgcc ggcgcaaatc ttggcagcag cagcgcgcac
      421 cagctcttct ttgtccggcg ccagccggta atgcagcgca agcgagtagc ctttgtcctc
      481 gaggatgatg ccgggaccag cctcggcgat ggcggcaaac tgccgcttca cggcgggatc
      541 aagcgcgggc agccgcggcg cttccggcgc ggcgccggga gtcgtgcgca gctcggcgcc
      601 gtgaccgccg atcgccggca attgcagggg ggagaagatg agatcgagct cgcttaccgg
      661 tcggccgctc acgaaagcga cgccgccatc ggtccgcttc cagagtcgcg cgagcgtatc
      721 gcgcagctcg tgcgacacga aaacctcacg cggagtggga gcgaggtcga gaatcgtccc
      781 gtcgacgtcg agcagaaagg cgaactgctt gagatcggca ggaagcgacg gaatggagtt
      841 tgacatcacg gttgtccgga aaggagtgcc attgcgtgcg gggcgcaggc ccagtgcgtg
      901 caaattaagg gagggtcacg cgttcggtac ggatacgaca actcatcgat tcaggggccg
      961 ttccgaacaa aaaaacggca cgcttgcgca attggcgttg tcataggcaa tctccaggca
     1021 gttaagagct gattgcagcc caagggtcat ctacgtccga atatatggtc tccggcacct
     1081 agagcgccat cccgccgtcg accaggtgcg gttgcccggt gatgtagctg gcctcgtcgg
     1141 aggccaggaa aaccgcgagc gccgccacct cctcgggccg gccgagccga cccaggggtt
     1201 ggcgttcgac gaacgccttc tccacctccg ccagcgattt accggtcgcg cgcgagcggt
     1261 ctttgatccg gtcctcgagc gagggtgatt cgatcgtgcc tggacagatt gcgttggcgc
     1321 ggatgccttg ccggatgaag tcggccgcaa ccgctttggt aaggccgatg accgcggcct
     1381 tggttgcgcc gtaggcatag cgatcgggaa ccccgcggat cgacgacacc gcggacgaaa
     1441 tgttgacaat cgagcccgcc ttcttctgca gcatgccggg gaggaatgcc ttgatcattc
     1501 gatgcatgga tttcacgttg agatcgaagg agaagtccca gtcctgctcg gagcagtcga
     1561 gcacgctgcc atgatgcacg tagccggcgc agttgacgag cacgtcgagt gcgccgaact
     1621 cggtcgcggt ctccttcgcg agcgcctcga tcgccgcggt cgagcgcacg tcgagcttgc
     1681 ggccctttac gcttttgatg ccttcaagtt tcttctcttc taaatcggtg gcgatcacgc
     1741 gggcaccttc gaccgcaaat gcttcggcga tggcacggcc gatcccttgt cccgccgccg
     1801 tgatgagcgc gagcttgcct tgcagacgta aagccatggc ttgttctccc tgccgtaggt
     1861 aacggcattc gcggcctttg gttcacgcgc aagcgcggtc cctggggtcc cggtcccgca
     1921 gccgactgtg cgtgagccg
//
LOCUS       DATLJT010000116         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2992148,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000116 DATLJT010000000
VERSION     DATLJT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992148"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..>2571
                     /gene="mgtA"
                     /locus_tag="VM910_02560"
     CDS             296..>2571
                     /gene="mgtA"
                     /locus_tag="VM910_02560"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HVI61468.1"
                     /translation="MADLFALGQLAAEPLCQRLRSSLAGLTCEEAACRLQDFGLNLVA
                     RERKPTILQEIWGRARNPLNALLLTLAAVSYFIGDMRAAIVIVLMVLLAVGTAFIQEH
                     RSNEAAARLRAMVKTTASVRRRPAVGDGDFAEVPIESLVPGDIVRLSAGDMIPADLRL
                     LEAKDLFINQSALTGEAMPAEKFCHSYQQEIKDPFDLPNMCFMGANVVSGFATGIVVR
                     TGAHTFFGQLADEIAGRRVPTAFDQGINRFTWLMIRFIIVMVPTVFLINGLTKHDWLE
                     ALLFAVAVAVGLTPEMLPMIVTVNLAKGAIDMSRARVIVKRLNAIQNFGAMNILCTDK
                     TGTLTQDRIILKRYLDIRGEESERVLQYAYLNSHFQSGLKNLLDIAVLQHVDMHGILG
                     IDKGFAKIDEIPFDFSRRRLSVVVSHGPDQHILICKGAVEEIFAVCSRYEIDGTTGRL
                     DESHFTTAKEETIALNSDGFRVVAVAYKELDASQTVYSAADERDLILLGCIAFLDPPK
                     ESAGAAIAALADKGVRVKILTGDNEVITRKVCREVKLDPGEILLGARIDAMTEDELAD
                     VAERTTVFAKLTPSQKERIVQALHRKGQVVGFLGDGINDSPALKAADVGISVDTAVDI
                     AKESADIILLEKSLMVLQEGVTEGRKIFANITKYIKMGASSNFGNMFSVLGASLFLPF
                     LPMAPIQVLTNNLLYDFSQTAIPTDNVDEEYLASPRRWDIDNIFKFMIFIGPISSIFD
                     YATYAMMLFVFDSWTNPSL"
BASE COUNT          518 a          788 c          755 g          510 t
ORIGIN      
        1 ggaaaggtct acctggggcg gcgcgaggtc gtcaccgaaa ggcccgaaga cgagttggcg
       61 gaagtcgagc gttggcggca atcggcgatg cgcgattctc gggacgcgcg ggccgatgca
      121 gctcccctgt cgctgccctg aagcggcggg agccgacgcg gacaacggca tggggttatg
      181 acgcgtgcgc gggccgcgca tccatcaatt gcagatcaaa tccatggatg ctgcatattc
      241 ttcgagccct cgacatcgag cccatccagg cgaaagggac cggtcaccct cccaggtggc
      301 ggaccttttc gcactaggtc agctcgcggc cgagcctttg tgccagcggc tgcggtcgag
      361 cctcgcgggc ctcacgtgcg aagaagctgc atgccggctc caggatttcg ggctcaacct
      421 ggttgcgcgc gagcgaaaac caacgatcct gcaagagatt tggggccgcg cgagaaatcc
      481 cctcaacgcg ctgctgctga cgctggcggc cgtttcctat ttcatcggcg acatgcgcgc
      541 ggcgatcgtc atcgtgctga tggtgctgct cgctgtcggc accgctttca ttcaggagca
      601 tcgatccaac gaggcggccg cccgcttgcg cgcgatggta aagaccaccg cgagcgtgag
      661 acggcggccg gccgttggag acggcgattt cgctgaggtc ccgatcgaat cgctggtccc
      721 aggcgacatc gttcgactgt cggccggtga catgatcccg gccgatctac gcttgctcga
      781 ggccaaggac ttgttcatca accaatccgc tctgaccggg gaagccatgc cggctgaaaa
      841 gttttgtcat tcataccaac aagagatcaa ggatccgttc gatttgccga acatgtgctt
      901 catgggcgcc aatgtcgtca gcggctttgc gactggaatc gtcgtgcgca ccggagcgca
      961 tacctttttt gggcaacttg ccgatgaaat cgcgggccgg cgcgtgccca cggccttcga
     1021 tcagggcatc aatcgtttca cctggttgat gatccggttc atcatcgtca tggtgcccac
     1081 cgtctttctt atcaacggct tgaccaagca cgactggctg gaggcgctgc tattcgccgt
     1141 cgccgtcgcg gtcggcctca cgcccgaaat gctccccatg atcgtaaccg tgaacctcgc
     1201 gaagggcgcg atcgacatgt cgcgcgccag ggtcatcgtc aaacggctca atgcgattca
     1261 gaacttcggc gcgatgaaca tcttgtgcac cgacaagacc ggcacgttga cccaggatcg
     1321 gatcattctc aagcgatacc tcgacattcg cggcgaagag tcggaacgcg ttctgcaata
     1381 tgcctatctc aacagccatt ttcagtctgg gctgaagaac cttctcgaca tcgccgtgct
     1441 ccaacacgtg gacatgcacg gcattctcgg aatcgacaag ggcttcgcga agatcgacga
     1501 gattcccttt gatttctcgc gacgccggct atcggtagtg gtgtcccacg gaccggacca
     1561 gcacatcctc atatgcaagg gggcggtcga ggaaattttc gcagtctgtt cgcgctatga
     1621 gatcgacgga acaacgggcc ggctcgacga aagtcacttc acgaccgcca aagaagagac
     1681 gatcgcgctc aattccgacg gtttccgggt cgtggcggtg gcttacaagg agctcgacgc
     1741 ctcgcaaacc gtatactcag ccgccgacga gcgcgacctg attttgctcg ggtgcatcgc
     1801 ctttctcgac ccgcccaagg aaagcgccgg cgcggcgatt gccgcgcttg ccgacaaggg
     1861 cgtgcgcgtg aagattctga ccggtgacaa cgaggtcatc acgcgcaagg tctgccgtga
     1921 agtgaaactc gatcccggcg aaattcttct cggcgcgcgg atcgacgcca tgacggagga
     1981 cgagttggcc gacgtcgccg aacgcacgac cgtattcgcc aagctgacgc cgtcgcagaa
     2041 agagcgcatt gttcaggcct tgcaccgcaa aggccaggtg gttggctttc tcggcgacgg
     2101 catcaacgat agccccgcgc tcaaggccgc cgatgtcggc atttccgtcg atacagctgt
     2161 cgacatcgcc aaggaatcgg ccgacatcat tctcctggaa aagagcttga tggttctgca
     2221 ggagggcgtc accgagggac gcaagatctt cgccaacatc accaaataca tcaaaatggg
     2281 cgccagctcg aatttcggca acatgttcag cgtgctcggg gcaagcctgt tcctgccgtt
     2341 cctgccgatg gcgccgattc aagtgctcac gaacaatctg ctctacgatt tctcccagac
     2401 cgcgattccg accgacaacg tcgacgagga gtacctcgct tcgccccgcc gctgggacat
     2461 cgacaacatt ttcaagttca tgatcttcat cggcccgatc agctcgatct tcgactatgc
     2521 gacttacgcg atgatgctgt tcgtcttcga tagctggacc aatccgtcgc t
//
LOCUS       DATLJT010000117         5943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_694341,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000117 DATLJT010000000
VERSION     DATLJT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694341"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..768)
                     /locus_tag="VM910_02565"
     CDS             complement(469..768)
                     /locus_tag="VM910_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61469.1"
                     /translation="MAHAQQLPSIESLSESYVACAQSAFVRRLDDFGANNLPQATERV
                     FLDCQLEEDALYTTAVASAPGNTQAIALVRAAVEQLKAKLKAELLAAPPTMPPKE"
     gene            1238..1564
                     /locus_tag="VM910_02570"
     CDS             1238..1564
                     /locus_tag="VM910_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVI61470.1"
                     /translation="MTIASLKSSQKPTATSEFLASNDVRALIAHQLGVDVKRVTDEAH
                     FADDLGADWLDRLELMIVIEDRFADVVITDEDVDQLEVVGDLIRHIETVDNERRRPST
                     VGAPLQ"
     gene            1949..2257
                     /locus_tag="VM910_02575"
     CDS             1949..2257
                     /locus_tag="VM910_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HVI61471.1"
                     /translation="MKSLVVKRSTVVAGHKTSVTLEDAFWNGLKEIASGRNITLSDLV
                     TAVDSERRQGNLSSAIRLFVLDFYRNQLADVKDGRDDAEGHLAVKVGAVTTRPNNGGP
                     "
     gene            3182..4768
                     /locus_tag="VM910_02580"
     CDS             3182..4768
                     /locus_tag="VM910_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61472.1"
                     /translation="MPAPSFADSDPPLWDGLGSVTYKISTSRAEAQAYFDQGLRLAYA
                     FNHGEAQRAFRKAQKLDPSCAMCFWGEALVLGPNINLPMQENAVGPAFAAVQRALALS
                     TNANPREQALIVALATRYTQEPKADRAPLDAAYAAAMGKLAADFPDDNEIALLYAEAV
                     MDLSPWNYWQPGGHEPNPQSAPIVPTLERVLAKEPNHPGAIHFYIHAVEASDRPQRAE
                     PHADRLRGAIPSAGHLVHMPSHIYYRVGRYLDALADNKIATTVDEKYLADTNAPMGVY
                     RLGYYPHNVHFVLASAQMAGDGATVIAAAEKLRGLIPDEAARDIAMVHPVKAAPYFAH
                     AQFSAPETILALPDPGDAMPYVKAMWRYSRGVAYAAQRNFDAAMAEADAIAALERATD
                     FSLLKASGVPGEEVMRLARAVIEGRVAQAQGNLSVAIARFEEAAALQDTLPYTEPPYW
                     YYPVRQTLAAALLQAGRLPEAEDQFQRALKRAPANGWSYYGLLQVYKARGDATAAAQA
                     EAELAKTWIGDRQLLQISNL"
     gene            complement(5218..5824)
                     /locus_tag="VM910_02585"
                     /pseudo
     CDS             complement(5218..5824)
                     /locus_tag="VM910_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            complement(5733..>5943)
                     /locus_tag="VM910_02590"
     CDS             complement(5733..>5943)
                     /locus_tag="VM910_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HVI61473.1"
                     /translation="TTGSLRAMRARPDGYTILMGHIGTHAFSVSLYPKLAYKPDVDFE
                     PIGFPTAYRNRGKEGLSGRRLEGVY"
BASE COUNT         1170 a         1765 c         1802 g         1206 t
ORIGIN      
        1 atggcgttgg tggcgcctgg aagataccgc ctaccgacga gaccttcgac gcgcgctacg
       61 gtcgatggtg agaccaccgg ctcgaaactc gggccgtacc gcttggcaag cctgcgacgc
      121 cgttgtcatc gcaggcgaca gttccgccat cgatgatgca gtagcgcgag cgcaggcgga
      181 cgagcgcctc gacaatcagt gggaagcggc gggtgaggtc attgcccgga cggctgtaga
      241 gcctctgaac gccgttcttc cgggcaataa accgcatgct tgatctcatg caggcattgg
      301 ctgcctgagg gcagcttatc gggtttggtt ggaaggcacg gcacgatgaa cccggccggg
      361 agggtgcgca cacgcaacat aatgctcata gctagggact atattcgcca taggcagggg
      421 tgatggtccg ttgggcatga tgggctctga gcgagcgcct atctggtgct attcttttgg
      481 gggcatcgtc gggggcgctg ccagcaattc cgccttcagt ttagccttga gttgttcgac
      541 cgcggcacgg accagggcga tagcttgagt attgcctggt gccgaggcaa ccgcagtcgt
      601 gtagagggca tcttcctcaa gctgacagtc gagaaacact cgttcggtcg cttgtggcaa
      661 attgttggca ccgaagtcat cgaggcgccg cacaaaggcg ctctgcgcac aggccacata
      721 ggattcactg agagattcga tgctgggcag ctgctgcgcg tgcgccaagg aggctccagc
      781 acatagcgcg gtgaccagga ctgcacgacg cgctctcccc gtcgagctgg acatgggcag
      841 gtacaaccac acaaacgcaa cgaagaacga acgatcgacg ggcggtcgct cgggaacggc
      901 acggcctttt ccgctgtggc gccttccgca gcggcccgtg tgcgacgccc atcaatccaa
      961 aatattcggg agcggtattt cgatcatgtc ccccgggccc gaatactcgg acgcttaaca
     1021 ttcagccgct tggtcagttc cgcccgcacc gcggctttct gttagcccga tccacattgc
     1081 ccagcagtgt ggcctgcttg ctacataccg cccggtaagt tgcctgtcag cttcgacgat
     1141 ttgtagatgc ataactgcgc gtataggtga gcaggactta acccgggcca cattgcgggg
     1201 cggatttcat ggtccgctgc gattcggggg ccttgtcatg accatcgcca gcttgaaatc
     1261 gagtcaaaag ccaacggcta cgagtgaatt ccttgcctcc aacgatgttc gcgctctcat
     1321 tgcccaccaa ctaggcgtcg acgtcaaacg tgtaactgat gaagcgcact tcgcggatga
     1381 tctgggggct gactggctcg atcgcctgga attaatgatc gtgatcgaag atcggttcgc
     1441 ggacgtcgta atcacggacg aggatgtcga ccagctagaa gtcgtcggcg atctgatccg
     1501 ccacatcgaa accgtagata atgagaggcg acgaccctcc accgtcgggg cgccgctcca
     1561 gtgatccgca agttatggtc gcgtttagct cgcgcgccgt ggagcccgcg gagcaacacg
     1621 accgctgcga gcagctagga tgcgcacgcg gccggcgaca tatgaggggc cggccttttt
     1681 cacgcgagga cctttcgagc gccccgagag ggtctgcacg tgcacggagt tccttgcttt
     1741 tggtaaggcc actgcctcga actgctcgcg ccaattgcaa tcatgaattc gtagcgcgag
     1801 cggcgatcaa gttgatggcc tgacctgctc gttgcactga tctgtgtgat ttttgcaacg
     1861 ctctagaatt cttttaggac agccacagta cgggacaaat ttcctattcg gatgttacga
     1921 atttgctaca acaggtgggg gcgtcatcgt gaaatctctg gtcgtcaagc gctctaccgt
     1981 ggtcgctggc cataagacga gcgttactct cgaagatgca ttctggaacg ggttgaagga
     2041 aatcgccagc gggcgaaaca ttaccttgtc ggacctagtc accgcggtcg attccgagcg
     2101 gaggcagggc aatctgtcat cggcgattcg cctcttcgtc ctcgattttt atcgcaacca
     2161 actcgccgac gtaaaggacg gacgtgacga tgcggaaggc catcttgcgg tgaaggtcgg
     2221 agccgttacc acccgcccca acaacggggg gccataagca agcgccgcga gcaagtagct
     2281 ctatttgttt ttttgagact tgggggcgaa gcaatgccct atctcatttg tcggtgcccg
     2341 gagacccaac ggcgggttga tcttcagctt tacacggact acgcgacgct agcccgcatc
     2401 tcgtccaatt cagtgcgctt tcaatgcccg cactgcggcg cggaacatga gacgaacgtg
     2461 ggagcagcgt ggcttgagga cgctctagga ggtatcagcg tggcgcacgt gagctgagca
     2521 gcctgttggc agggacgctc agcacgttga tcctacagga gcgacgcgat gcaggcctgc
     2581 cattccgcat cgtcctcgag cagcatgctg agcaccgcga tgcgccgcat gccgcatgct
     2641 ctcgcgttgc cgcgcacgcg ccgcgagcgg ccacgccacc gcgccgccga gcagcgtgat
     2701 gaactcccgg cgtctcatag tcgccaccag aggtcaaaac cacgcaccgc atcgtgtcac
     2761 agcgatctgg atcttggaac ggggtgagag ggatgtgaac tgcgaccaat tgtttcggca
     2821 cggtgacatg cgtggagtaa gcgcgtaggc tcggcaaatg tgttgatctg agcgaagcag
     2881 gctagtccgt ctcggcgggt tcttggaggg gatcatgacg catttgtcgc ggctaactct
     2941 gttttccacc actgccatta gccttggggg cattgtcttt ctcggttcgc tcaccggtga
     3001 ggccatatca gggggcagag gaccgctgat gggcgcggcc agggcgacaa cagtcgctcc
     3061 cgcgttatcg agttcggatt ttcctatctg ccatacgtct gctgttgctg agcgtacgaa
     3121 tacagtgctc cgtcttgcgc agacgcggac cgaagtgccg ccgactgaaa tgaaagcggc
     3181 cgtgccagcg ccctcctttg ccgacagcga cccgccgctc tgggatggcc tcggctccgt
     3241 aacttacaag atcagtacgt caagagccga ggcacaggcc tacttcgacc agggcctccg
     3301 cctggcctat gcctttaatc atggcgaggc gcagcgcgcc ttccgcaagg cgcagaagct
     3361 cgatccctca tgcgccatgt gcttctgggg cgaggcactg gtgctcggcc ccaacatcaa
     3421 ccttcccatg caggaaaatg cggtcggtcc ggcctttgca gcggttcaga gggcgctcgc
     3481 gctcagcacc aacgcaaacc cgcgcgagca ggcgctgatc gtcgcgctgg caacgaggta
     3541 tacgcaagag ccaaaagcgg atcgcgcccc gcttgatgcg gcctatgcgg cggccatggg
     3601 gaagcttgcg gccgacttcc cggacgacaa cgagatcgcg ctgctctacg ccgaggcagt
     3661 gatggacttg agcccatgga attattggca accgggcgga cacgagccga atccgcaaag
     3721 cgccccgatt gtgccgacgc tcgagcgcgt gctggcgaag gagccgaacc atcctggcgc
     3781 cattcatttc tacatccatg cggtcgaggc gtcggatcgg ccgcaacgtg cagagcccca
     3841 tgcggatcgg ctgcgcggcg ccattccgag cgccggtcac ctcgtgcaca tgcccagcca
     3901 tatctattat cgtgtcggcc gctatctcga tgcgctcgcc gacaataaga tcgcgaccac
     3961 ggtcgacgaa aaatacttgg ccgacaccaa cgcgccaatg ggtgtctatc ggctcggcta
     4021 ttacccgcat aacgtccatt tcgttcttgc ctcggcgcaa atggcgggcg acggagcaac
     4081 tgtcattgcg gcggcggaaa agcttcgcgg tctcatccct gatgaggcgg cccgcgacat
     4141 tgccatggtg catcccgtaa aggcggcgcc atacttcgcc catgcccagt tcagcgcgcc
     4201 cgagacgatc ctggcattac ccgatcccgg cgacgccatg ccctatgtca aagccatgtg
     4261 gcgctactcg cgtggcgtcg cctatgcggc acagcgcaac ttcgacgcgg ccatggctga
     4321 ggctgacgcg atcgcagccc ttgagcgagc aacggatttc tcgctgctca aggcatcggg
     4381 ggttccgggc gaggaggtga tgcgccttgc gcgcgctgtg atcgaaggtc gcgtcgctca
     4441 agcgcagggg aatctctcgg ttgcgattgc tcgtttcgaa gaagctgcgg ctctccagga
     4501 taccctgcca tacacggaac caccctactg gtactatccg gtgcgccaaa cgctcgcggc
     4561 cgcgctgctg caggccggcc ggcttcccga ggcggaggat caattccaac gcgcgctcaa
     4621 gcgcgccccc gcgaatggct ggtcctacta tgggctcctc caagtctata aggcgcgtgg
     4681 cgatgccacg gctgcggccc aggccgaggc tgagctggca aagacctgga tcggggatcg
     4741 ccaattgctg cagatctcaa atctttagtg ggaaaatcgc gccaaacctc gtgcagcatc
     4801 tgagagctca tcccgaaaag ctttctccag cgggtttgtt gactatgctc gcaaagtcta
     4861 cgacgccgtc cggcgacgta atgttttccg gtttgccccc caaagcggac ctccgatctg
     4921 cgcgttagtg agtgcacgcc ctagtcgcac cgcgagatca ccgggccgcc gggacctgcg
     4981 ctccagcaaa tgtgggacac cacgcgcacg cccccaacag aaaaattctt gtcctctacc
     5041 tttcggtccg acctcgtctg aataggtcgc gccagccgac ttgagattat ctgcgaggtc
     5101 acttcctccg gtcatgggcg atggcagcga gcggcggagc accgcgattg aaatgtcgcg
     5161 tgcaccggct ggccgggaat tcgcggaacg ggatttatgg aatgtcccga gtcggcctca
     5221 ccctattgtg acgttcgccg ctttgatgat cggtgtccaa cgagcaatct cgctttttac
     5281 caatgctgcg agtggcttct ggcctcgcct ttctttgccc ggaatgtcac cgccgaggtc
     5341 ggagaaacgc ttgcgtacct gctcgtcatc gagtgccttg tcgagtgcat ccggtagcgc
     5401 gtcgagagtc ggttgcggta cgcccttcgg cgcaaacaac gcccaccaag gcaacgcctg
     5461 aaattcaggc aggccggcct ctattgtagt tgggacattt gataggatcg ggtgacgttc
     5521 ggtagcgacg actgcatacg ccttaatggt cccggtctga acctgctgtc cgatgtctgc
     5581 ggttccgttg cacatgtaat cgacttgacc gccgaggcac catcgttggc ttgataccca
     5641 gcagcgaatt aagcaagagg gggagggtaa atgtgattga gccgacgccg gcgtggccca
     5701 tattcaactt ctcggcattg acctttgcgt aactaataaa ctccttcaag tcttctgccg
     5761 gaaagtcctt ccttgccacg attacggtag gctgttggaa aaccaatcgg ctcgaaatcg
     5821 acatctggtt tgtaggcaag tttggggtag agcgagactg agaaggcatg agttcctatg
     5881 tggcccatca ggatggtgta gccgtcaggt cttgcgcgca ttgcgcggag tgagccggtc
     5941 gta
//
LOCUS       DATLJT010000118         1984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3681273,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000118 DATLJT010000000
VERSION     DATLJT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681273"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VM910_02595"
     CDS             complement(<1..575)
                     /locus_tag="VM910_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVI61474.1"
                     /translation="MNALADFLLLGIDGGGSRCRARLCNISGTTLSEGVAGAANIRLG
                     VEQSFASVLQATIQCMSGAGLSSRDFKRVVACVALAGASEPSQLEIARRHEHPYRIAV
                     FVTDAQAACVGAHGGRDGGIIVIGTGSIGWAELNGQQYQVGGWGWPISDEGSGAWLGC
                     EALRRTLWAHDGRMPWSALLRSLFAKFRSDP"
     gene            662..1588
                     /locus_tag="VM910_02600"
     CDS             662..1588
                     /locus_tag="VM910_02600"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI61475.1"
                     /translation="MDTERASPRYSDLDVWAPLDIVEAMIEGQFTAVAAVRAARFVLL
                     EAALAAAERLRKGGRLVYVGAGTSGRLAVQDGAELVPTFSWPQERLLLFIAGGRNALL
                     QSVEGAEDAVDQAARLTQQHEIGSADVVIAVAASGTTPFTLSCLTEAKRRGALTIGVA
                     NNRDTPILKEADHAILLDTGAEPIAGSTRMKAGTAQRITLNVFSSLVMILLGRVYDGL
                     MVDLQAVSQKLIRRSEGILTRLTGSSTEQAREALHRANGNVKLAVLLLHGCDVKEATD
                     VLHRAGGQLRAALADIGKPGPEPADLSDLPTS"
     gene            complement(1717..>1984)
                     /locus_tag="VM910_02605"
     CDS             complement(1717..>1984)
                     /locus_tag="VM910_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61476.1"
                     /translation="FRRPAMLSQRLYYLGRRSAMAQVITRCRLTGHYMFMGMDVDPKE
                     FTRSLGPFARKFCPFCACDHLWHKEDSKFLASKAALRPHIQQAS"
BASE COUNT          413 a          617 c          597 g          357 t
ORIGIN      
        1 tgcggatccg accgaaattt tgcaaaaagc gaacgcagca gcgccgacca cggcatgcgc
       61 ccatcatggg cccacagcgt gcggcgcaac gcctcgcagc caagccaagc gccgcttcct
      121 tcgtccgaaa tcggccaacc ccagccgccg acttgatact gttgcccatt cagctccgcc
      181 cagccaatgc tgccggtgcc gatcacgatg atgccgccgt cacgcccgcc gtgtgccccg
      241 acgcatgccg cctgggcatc ggtgacgaaa accgctatgc gatagggatg ttcgtgccgt
      301 cgcgcaatct cgagctggct gggttcgctg gctcccgcca gcgccacgca ggccacaacc
      361 cgtttgaaat cgcgcgacga caatcccgcc ccactcatgc attggatcgt ggcctggagg
      421 accgaggcaa agctttgctc cactccaagg cgaatattgg ccgcgccagc gaccccttca
      481 cttagcgtcg tgcccgagat attgcaaagg cgggcgcgac aacggctgcc gccgccgtcg
      541 atgccgagca aaaggaagtc cgccagggcg ttcatttcca atgctccgtc aatcccctaa
      601 cgcgcgagaa ccaatccgga gcgaaaagcg ttcttctgcg tgtcaacact ctgcgtgatc
      661 catggatacc gaacgcgcga gcccgcgata ttctgatctc gatgtttggg cgcctttgga
      721 catcgtcgag gcgatgatcg agggtcaatt taccgcagtc gccgcggtaa gagcggcccg
      781 cttcgtactt ttggaggctg cgcttgctgc cgccgaaaga ttacgaaaag gcggtcggct
      841 ggtctatgtc ggcgctggaa cctctggtcg actggccgtc caggacggcg ctgaactcgt
      901 tccgaccttc agctggccgc aagagcggct gttacttttt atagctggcg gccggaacgc
      961 gttgctgcag tcggtggaag gcgccgagga cgcggtcgat caagctgccc ggctcaccca
     1021 acaacacgaa atcggctctg cagacgtggt catcgcagtc gctgcgagcg gcaccacgcc
     1081 gttcaccttg tcctgcctaa ccgaagcaaa gcgacggggc gctttgacaa ttggagttgc
     1141 aaacaatcgc gatacgccga tcctgaaaga ggccgatcac gcaatcttgc tggataccgg
     1201 cgcggagccg attgcgggat ccaccagaat gaaagcggga acggcgcagc gtatcaccct
     1261 caatgtcttc tcgtctttgg tcatgatctt gctggggcgg gtctacgatg gactgatggt
     1321 ggacctacag gcggtcagtc agaagctcat acgacgcagc gaaggcatcc tcacgcggct
     1381 cactggcagc agcaccgagc aggcgcgcga ggcgttgcat cgtgcaaacg gcaacgtaaa
     1441 gcttgccgtg ctgcttctgc acgggtgtga cgtgaaggag gcaacagacg ttctccaccg
     1501 cgccggtggg caactacgtg cggcactcgc cgacatcggc aagcccggtc ccgaacccgc
     1561 tgatctgagc gatctgccga cgtcgtgatg ggaccggcgc cagaccaaag gaccggtcaa
     1621 cagacatagc gttgtgaccc gagcgtctgg cgctggtata agatcgcgaa gcattcctcg
     1681 ctatggtttg gactcgtctt gggagggcgg gagcgatcag ctcgcctgtt gaatatgggg
     1741 ccgcagcgcg gccttggagg ccaagaactt cgagtcctcc ttgtgccaca agtgatcgca
     1801 ggcgcagaac gggcagaact tgcgcgcaaa cggaccgagt gaacgcgtga attccttggg
     1861 atcaacatcc atgcccatga acatgtaatg accggtgagc cggcagcgcg tgatgacctg
     1921 agccatcgca ctcctccgtc ctaaataata aagccgctga gacaacatcg ccggtcggcg
     1981 aaag
//
LOCUS       DATLJT010000119         4084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2303162,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000119 DATLJT010000000
VERSION     DATLJT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303162"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VM910_02610"
     CDS             <1..778
                     /locus_tag="VM910_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI61477.1"
                     /translation="GSDLAALRTSASRDGEEWVINGQKIWTTTWWGKYMFLAARTDRD
                     ARPPHAGISMFIVPMDAKGITIRPATTMYDGSFANIFYDDVRIPLENIVGEVNGGWKV
                     LTGALAFERGLVGGGIVLKVAHAFEQLRAHVMAGEGDAPPLSEDPIVRDRMAVLASEI
                     EVGRLLMMQCAELAADGITPPEYGAISKVFSGELMERFGEAALDILGMRATLSEGMPG
                     ALDNGRFEQNLRHSLMWVISIGTNEIQRSLIAQRGLGLPR"
     gene            789..2018
                     /locus_tag="VM910_02615"
     CDS             789..2018
                     /locus_tag="VM910_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI61478.1"
                     /translation="MQAEEKMTAPLEGIRVLDFSIMLAGPYCARLLADVGADVIKIEP
                     PEGDDMRLRTPLRDGHSAYFGQLNAGKRSLALDLKNAEAIKLVHRLVAEADIVVENFR
                     PGVMDRLGLGYDSLREINPRLIFCSISGYGQSGPDAERAAYAMIVHAESGFDRSLMRY
                     TGDRDRPAAGAIFVADVLGGIFGYSAIQTALVQRARTGHGQRIDVALMDCMLNLLVYE
                     LQEAQFPIRAPRPTYGPVRARDGDILIAPVTARNFTALCEVTGQSELANDSRFNALPA
                     RGANWTAMMQVIEKWTERQSVDECMAALDAAGVPCARYRDPGAALTDPHLLARGAFAT
                     ICDGAGEFFGVNAPWKMSGAETSVRSEIPAIGEHRDEVLSRALGLSPGAIAGLARTGA
                     FGPSKEKQRDQARGLPG"
     gene            2086..3321
                     /locus_tag="VM910_02620"
     CDS             2086..3321
                     /locus_tag="VM910_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI61479.1"
                     /translation="MLDMALDNALADHKTYGNVEMQHRLFARLRREDPVHWTEPAGFR
                     PFWTVTKYADIIEIERQADRFINAPRTKLLSVEFESKVREAMNGRPMLVRALPQMDNP
                     DHAKYQKLTHAWFQPRQLRTLSDRMEALAKESVDRMFTIGGECDFYNEIAIYYPLRVL
                     MTILGLPREDEERLLKITQAYFGGGDPDMQKGSDLIDATLAYVDYFKDVARERRQNPK
                     DDVATVIATSEIDGRPIEDFEAYSYYVALASAGHDTTSATIAGGLLALIENPEAMARL
                     QRDGDLIPDAAEEMVRWVSPVKHFFRTAAVDYEIRGKKIKPGDSLFISYPSGNRDEEA
                     FDRPFEFIPDRSPNRHLGFGFGIHACIGRHLAKAEIVAFFREFLKRVDRVELAGKPAW
                     TETSFLGGVKRLPVRYRAR"
     gene            3352..3663
                     /locus_tag="VM910_02625"
     CDS             3352..3663
                     /locus_tag="VM910_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61480.1"
                     /translation="MDLRELLSIPYLLEAEAVESEPGKWLLRLAFPELPGCTAEGFVV
                     EEVLRELERRRVEMIVTLVEAGKEPPVPRQPLAASDPLWTANDLGLSARVAALLGNAT
                     R"
     gene            3701..>4084
                     /locus_tag="VM910_02630"
     CDS             3701..>4084
                     /locus_tag="VM910_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI61481.1"
                     /translation="MLSREDNERLVRVGKGTPLGELFRLYWIPFLPAKDLVKDGQPQR
                     IRLLGEDLVAFRDSEGRIGLVDQACPHRGAPLIFGRNEDCGLRCVYHGWKFDVSGAVT
                     DMPAEPVRSRLKEHVRIKAYPCRERN"
BASE COUNT          772 a         1238 c         1323 g          751 t
ORIGIN      
        1 gggctccgac cttgccgcct tgcgcaccag cgcctcccgc gatggcgagg aatgggtcat
       61 caacggccag aagatctgga ccacgacctg gtggggaaaa tacatgttcc tcgccgcgcg
      121 caccgacagg gatgccaggc caccgcatgc cgggatcagc atgttcatcg tgccgatgga
      181 tgccaagggc attaccatcc gtcccgcgac cacgatgtat gacggctcct tcgccaatat
      241 cttctacgat gatgtccgca ttccactcga aaatatcgtc ggcgaggtca atggcggttg
      301 gaaggtgctg accggcgcgc tcgccttcga gcgcggcctt gtcggcggcg gcattgtgct
      361 caaggtcgcg catgccttcg agcaattgcg ggcgcatgtg atggccggag agggcgacgc
      421 gccgccgctt tcggaggatc ctatcgtccg cgacaggatg gctgtgctcg cgtccgagat
      481 cgaggtcgga cgcctcctga tgatgcaatg cgcggagctt gccgcggacg gcatcacgcc
      541 gccggaatat ggcgcaatca gcaaggtgtt ttccggcgaa ttgatggagc gcttcggcga
      601 agcggcgctc gatatcctcg gcatgcgcgc cacgctttcg gaaggcatgc cgggtgcgct
      661 cgacaacggg cggttcgagc agaacctgcg ccattcgctg atgtgggtca tctccatcgg
      721 caccaacgaa attcagcgca gcctcattgc gcagcgtggc ctcgggcttc cgcgatagga
      781 gatttgggat gcaggcagag gagaagatga ccgcgccgct cgagggcatc cgcgtgctcg
      841 atttctcgat catgctggcc ggtccctatt gcgcgcggct gctcgccgat gtcggcgccg
      901 atgtcatcaa gatcgagccg ccggaaggcg acgacatgcg gctgcgtacg ccgcttcgcg
      961 acggccacag cgcgtatttc ggccagctca atgccggcaa gcgcagcctc gcgctcgact
     1021 tgaagaatgc cgaggcgatc aagctggtac atcggctggt cgcggaagcc gacatcgtcg
     1081 tcgagaattt ccgtcccggc gtgatggacc gcctcggcct cggttatgac tcgctgcgcg
     1141 agatcaaccc gcggctgatc ttttgctcga tctccggtta cggacaatcc ggacccgatg
     1201 cggagcgcgc ggcctatgcg atgatcgtcc atgcagaaag cggcttcgac cgttcgctga
     1261 tgcgctatac cggcgaccgc gaccggcccg cggcgggcgc gatcttcgtc gctgacgtgc
     1321 tcggcggcat cttcggctat tccgcgatcc agaccgcgct ggtgcaacgc gcgcgcaccg
     1381 gccacgggca gcgcatcgac gtcgcgctga tggattgcat gctgaacctg ctcgtttacg
     1441 agttgcagga agcacagttc ccgatccgcg cgccgcgtcc gacctatgga ccggtgcggg
     1501 cgcgcgacgg cgacatcctg atcgcgccgg tgactgcgcg caacttcacc gcgctttgcg
     1561 aggtgacggg acaaagcgaa ctcgccaacg attcacggtt caatgcgctt ccggcgcggg
     1621 gcgcgaactg gaccgcaatg atgcaggtca tcgagaaatg gaccgagcgc caaagcgtcg
     1681 acgaatgcat ggcagcgctg gatgccgcgg gcgtgccttg cgcgagatac cgtgaccccg
     1741 gtgcggcact gaccgatccg catctcctcg cgcgcggggc gtttgcgact atctgcgacg
     1801 gagccggcga attttttggc gtgaatgcgc cctggaagat gtcgggcgcc gagacatccg
     1861 tccgttccga gatacccgcg atcggtgagc accgcgatga ggtgctgtcg cgcgcgctcg
     1921 gcctgtcgcc tggggcgatt gcggggctcg cgaggaccgg cgcatttgga ccgtcgaaag
     1981 aaaagcaacg cgaccaggca aggggtctgc cgggatgacc tcatgacgct gaactcgcac
     2041 acatgaaagc gagggcgtcg catcaatcaa gcggagggaa cagaaatgct cgatatggca
     2101 ctggacaatg cgctcgccga ccacaagacc tacggcaatg tcgagatgca gcaccgattg
     2161 ttcgcccggt tgcggcgcga ggatccggtc cattggactg aacccgcggg ctttcgtccg
     2221 ttctggacgg tcacaaaata tgccgacatc atcgagatcg agcgccaggc cgatcgattc
     2281 atcaacgcgc cgcggacaaa gcttctcagc gtcgagttcg agagcaaagt cagggaagcc
     2341 atgaatggca ggcccatgct ggtgcgggcg ctgccgcaga tggataatcc cgaccacgcc
     2401 aaatatcaga agctgacgca tgcatggttt cagccgcgcc aactccgcac gctttcggat
     2461 cgcatggagg cgctggcgaa ggaaagcgtg gaccggatgt ttacgatcgg cggggagtgc
     2521 gatttctaca acgagatcgc gatctattat ccgctgcgcg tcctgatgac gatcctcggc
     2581 ctgccgcgcg aggacgagga gcggctgttg aagatcacgc aagcctattt cggcggcggc
     2641 gatccggaca tgcaaaaggg ctccgacctt attgacgcaa cgcttgcgta cgtcgattac
     2701 ttcaaggacg tcgccaggga gaggcggcag aatccaaaag acgatgtcgc gacggttatc
     2761 gcgacctcgg agatcgacgg aaggccgatc gaggatttcg aggcctattc ctattacgtg
     2821 gcgctggcga gcgccggcca cgacaccacc agcgccacga tcgccggcgg cttgctcgcc
     2881 ctgatcgaaa atccggaggc gatggcccgc ctgcagcgcg atggcgatct tatccccgat
     2941 gccgccgagg aaatggtgcg ctgggtatcg ccagtcaagc acttcttccg gaccgcggcg
     3001 gtggactacg aaatccgcgg caagaagatc aaaccaggag acagcctctt catcagctac
     3061 ccttccggca atcgcgacga agaagccttc gatcgtccgt ttgaattcat tcccgatcgc
     3121 tcacccaacc gtcacctcgg ttttggcttc ggcattcacg cctgcatcgg caggcacctc
     3181 gccaaggccg agatcgtggc gttcttccgc gagtttctca agcgggtcga tcgcgtcgag
     3241 cttgcgggca agccagcctg gaccgaaact tcattcctcg gcggggtgaa gcgacttccg
     3301 gtgcgatatc gggcgcgata gctggagtcg cggacttctt gggaggatcg cttggatctg
     3361 cgtgagctat tgtcgattcc ctacctgctc gaggccgagg ccgtcgaaag cgagcccggc
     3421 aaatggctgc tgcggcttgc ctttccggag cttccaggtt gcaccgcgga aggtttcgtc
     3481 gtcgaggagg tgctgagaga gctcgagcgg cggcgggtcg agatgatcgt cactctcgtg
     3541 gaggcgggca aagagccgcc cgttcccagg cagccgcttg ccgcatccga tccgctctgg
     3601 accgccaacg atctcggttt gtcggctcgc gtcgctgctc tcctgggaaa cgcgaccaga
     3661 tagctagttg gaacaccgct ggattcaatg gagaaagctc atgctctcgc gcgaagacaa
     3721 tgaacggctg gtgagggttg gcaaggggac gcctcttgga gagctgttcc ggctctactg
     3781 gattcccttt ctgcccgcga aggatctcgt gaaagacggg cagccgcagc gcatccgcct
     3841 gctaggcgaa gatctggtgg ccttccgaga tagcgaaggt cgcattggtc tagtcgacca
     3901 ggcctgtcca catcgcggcg cgccgctgat tttcggccgc aatgaggatt gcgggctgcg
     3961 ctgcgtctat cacggctgga aattcgacgt gagtggtgcg gtgacggata tgccggccga
     4021 gccggtgcgc agccgactca aggagcacgt ccggatcaag gcttatccct gtcgggaacg
     4081 gaac
//
LOCUS       DATLJT010000120         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_235157,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000120 DATLJT010000000
VERSION     DATLJT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235157"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VM910_02635"
     CDS             complement(<1..1191)
                     /locus_tag="VM910_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVI61482.1"
                     /translation="MSVASETLVDVATEPAGGGRLWLSLRYALRDLRGGLRGFYVFIA
                     CIALGVLAIAGVGSVAASLSDGLAREGRNLLGGDVAFVLFQREAKPAELAFLRSRGQV
                     SVIATLRGMARSSDDRLALVEMKAVDGSYPMLGELSLDPALPLQDLLAEHDGSFRAAA
                     DPALLARLDLKIGDEVTIGDASFQIRSAVNAEPDKLSGGVGFGPRFLISDAGLRATAL
                     LQPGSLVRWSYRVRLPDNAADDRAVSALIDDARQAAPEAGWEVRSRSNASPQLERTIS
                     RFTQFLTLVGLAALLVGGVGVANAVKSHIDRRLDVIATFKALGATGRDVFSIYLTQVI
                     LLAALGSVIGLAAGAALPFVIVGLFGKLLPLPVVPALHASELALSFVYGLLTALAFGL
                     WPLGR"
     gene            complement(1188..1922)
                     /locus_tag="VM910_02640"
     CDS             complement(1188..1922)
                     /locus_tag="VM910_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61483.1"
                     /translation="MDSLIESPAQTGVAPDTIAISNVNLSLGTGAARVHILKDISLRV
                     ASGEAIGLIGPSGSGKSTLLMVMAGLERPDSGQVVVNGTPFNALDEDALARFRGRQVG
                     IVFQSFHLIPTMTALENVAVPLELAGNGDAARRAAQELSSVGLGDRLHHYPAQLSGGE
                     QQRVALARALAPDPAILVADEPTGNLDEATGKQIIELLFTKHAERGMTLVLVTHDASL
                     AQRCDRVVRLRSGRIEAGGADRKAAS"
BASE COUNT          366 a          663 c          669 g          303 t
ORIGIN      
        1 gcggccgagc ggccatagtc cgaaggcgag cgcggtgaga aggccgtaga cgaacgacag
       61 cgcgagctcg ctcgcgtgca gtgccggcac taccggtagc ggcagcagtt tgccgaacag
      121 gccgacgatg acgaagggca acgccgcgcc ggcggcaagt ccgatcaccg agccgagggc
      181 cgccagcagg atcacctgcg tcagatagat cgagaacacg tcgcggccgg ttgcgccaag
      241 cgccttgaat gtcgcgatca cgtcgagacg ccggtcgatg tgactcttga cggcgttcgc
      301 cactccgacc ccgccgacca acagcgcggc gaggccgacc aatgtcagga actgggtgaa
      361 gcggctgatg gtgcgctcga gctgcggcga agcgttggag cggctgcgca cttcccaacc
      421 ggcctcgggc gccgcctgac gggcgtcatc gatcagcgcg gatacggcgc gatcgtcggc
      481 cgcattgtcg ggcagtctga cgcgatagct ccagcgcacc aggctgccgg gctgcagcag
      541 cgcggtggcg cgcaggcccg catcgctgat cagaaatcgc ggaccgaaac cgacgccgcc
      601 ggaaagcttg tccggttcgg cgttgaccgc gctgcgaatc tggaagctgg cgtcgccgat
      661 cgtcacctcg tcgccgatct tcagatcgag ccgcgccagc aaggctggat cggccgcggc
      721 gcgaaagctg ccgtcgtgct cggcgagcaa atcctgcagc gggagcgccg gatcgagcga
      781 gagctcgccg agcattggat agctgccgtc gaccgccttc atctcgacca gagccagccg
      841 gtcgtcagag ctgcgcgcca tgccacgcag cgtcgcgatc accgaaacct gtccgcgcga
      901 gcgcagaaac gcaagttcgg cgggctttgc ttcgcgctgg aacagaacga aggcaacgtc
      961 gccgccaagc agattgcgac cctcgcgcgc caatccgtcg gacaggctgg ccgcgaccga
     1021 gccgacgcct gcgatcgcca agacgccgag cgcgatgcag gcaatgaata cgtagaagcc
     1081 gcgcaagccg ccgcgcagat cacgcagggc atagcgcagg ctgagccata agcggccgcc
     1141 tcccgcgggc tcggtcgcga cgtcgaccag agtttcggac gcgacgctca tgaggccgct
     1201 ttgcgatccg cgccgcccgc ctcgatgcgg ccggagcgca gccgcaccac gcggtcgcag
     1261 cgttgcgcga gggaagcgtc atgcgtcacc agcaccagcg tcatgcctcg ctcggcatgc
     1321 ttggtgaaga gcagttcgat gatctgcttg cccgtcgctt catcgagatt gccggtgggc
     1381 tcatcggcga ccaggatcgc gggatccggc gcaagcgcac gcgcaagcgc cacgcgctgc
     1441 tgctcaccgc cggacaattg ggcgggatag tgatgcagcc ggtcgccgag tccgaccgac
     1501 gacaattcct gtgcggcacg cctggccgca tcgccgttgc cggcaagttc cagcggcacc
     1561 gcgacgttct ccagcgccgt cattgtgggg atcaaatgaa acgattgaaa cacgatgccg
     1621 acctggcggc cgcggaagcg cgccagcgca tcctcgtcga gggcattgaa aggcgtgccg
     1681 ttgaccacca cctgtccgct gtcaggacgt tccaggcccg ccatcaccat cagcagcgtg
     1741 gatttccccg agcccgaagg cccgatcagg ccgatcgctt cgcccgatgc gacgcgcagg
     1801 ctgatatctt tcaggatatg aacgcgcgcg gcaccggtgc cgagcgagag gttgacgttc
     1861 gagatggcaa tcgtgtccgg cgcgacgccg gtctgtgcag gagattcgat gagactgtcc
     1921 atggcccggt catatggcac tttcgcttcc ctggtcgaga ggtggctttt cctgttcccg
     1981 cacatgatcg tgttgacatt g
//
LOCUS       DATLJT010000121         4638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1843464,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000121 DATLJT010000000
VERSION     DATLJT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843464"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VM910_02645"
     CDS             complement(<1..634)
                     /locus_tag="VM910_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61484.1"
                     /translation="MRSLAAALVFAVICGSTDATCAADWPTRPVRIIAPSTPGGAADM
                     FGRLVCDHFSETFHERCFVENRAGAGGLIGMAAAAQAAPDGYTLATSSIAYNVIAPAV
                     SPNPGFDPLKDFTHIAYIGGPPNVFVVNPALNVRSIAELVALGRRGPAIDYVSPGVGT
                     LGHLMAEVFAQKAGIKLQQIMTKGGSQAMMDLISGNVNVGTMTWTSALAQI"
     gene            788..1330
                     /locus_tag="VM910_02650"
     CDS             788..1330
                     /locus_tag="VM910_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HVI61485.1"
                     /translation="MDADLLTEGDDTIELKGSSIIAAIKASAIEFILSVPDIVTSGGL
                     LRPISRDRAFKLIRVCKEDECIGIASGLFYCDKRALSLIQYTGFLDSINAIRGVAVEY
                     RQPICMMVGLLGHDPSTTPRNSPRYGVRIIEPILDAMGIPHHLINEDADVAKIRPAIE
                     DAYATSHPVALLIGRRPSAS"
     gene            1327..1890
                     /locus_tag="VM910_02655"
     CDS             1327..1890
                     /locus_tag="VM910_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI61486.1"
                     /translation="MMKRDECFRALARHVTDEIVVATYSSAVDWLALGERALNYFSVG
                     AMGLDSSHALGLALGRPDKRVICLQGDGSLLMNLGCLVTIAAAAPKNLLHMVVQNGTY
                     EANGSHPIPNTKVEFAAMARAAGYAHVHEFSELANFEQQAAHVLKQDGPVFATLHVER
                     SKPLTYDYANLYDPARRVALKAALRAG"
     gene            2095..2577
                     /locus_tag="VM910_02660"
     CDS             2095..2577
                     /locus_tag="VM910_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61487.1"
                     /translation="MPRTPRNAKKRKKPDARYDAFRDEWERRRKGPDRGGGDWLVERP
                     DLPVDPLPFLQTLRRLFYFTQDSRFIELHRHLIGSDLIDPVTEKWSLFGTKLTRTLFE
                     LIEEEIRAGTTERQAIADAVAAFVLFPDAQSFDAAWKHLERRLREWRKALDKNQPKKV
                     "
     gene            2640..2834
                     /locus_tag="VM910_02665"
     CDS             2640..2834
                     /locus_tag="VM910_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61488.1"
                     /translation="MSDRTLVKLGRRYPGTSRMTRWRWRRDPDFPDPVVINGTEYYDS
                     DELTAYEEARRRRRPQKSSP"
     gene            3167..3754
                     /locus_tag="VM910_02670"
     CDS             3167..3754
                     /locus_tag="VM910_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61489.1"
                     /translation="MRELPQTRPLAAHIWAKDPDGFYVEPEWVDERLFDVESFAGIIM
                     DPACGIGHIPDAARAAGYQTIAIDLVDRGYEHFDGVADFLCRDRRVANIVCNPPYYIC
                     RNFALHALRLAYRKVAMIWLLRRLNAATWLRATPLRAVYLLSPRPSMPPAQVILAGEK
                     PGGGRQDFCWLVWERGYIGAPELHWLCRDGDDHAA"
     gene            3900..4502
                     /locus_tag="VM910_02675"
     CDS             3900..4502
                     /locus_tag="VM910_02675"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HVI61490.1"
                     /translation="MSDLPAKYHFHELKRLLGDYKLKVYMPCFHCWEPEAAEILFAFA
                     KFGGAAHEQLPSGTLPKMDALFAPDPEPENLFMLRDYVLSGDTIVAAAIKAAGGIRLL
                     ARKLGIRPHAIEQWKCIPTEQLQAIARITGLPQHVLRPDLFDVSEPQFYCTEEEADEI
                     WKWSHAWQLWLPLPYPPGFLPACCCARRPRLTLVVDRGAA"
BASE COUNT          901 a         1476 c         1394 g          867 t
ORIGIN      
        1 ggatttgcgc cagcgccgag gtccaggtca tggtgccgac gttcacgttg ccgctgatga
       61 ggtccatcat cgcctgcgag ccgcccttgg tcatgatctg ctgcagcttg atgccggcct
      121 tctgcgcgaa gacttccgcc atcagatgtc cgagcgtccc gacgccgggc gaaacgtaat
      181 cgatcgccgg cccgcgccgc cccaacgcca cgagctccgc aatcgagcgc acgttgagcg
      241 ccgggttcac cacgaaaaca ttgggcggcc cgccgatata ggcgatgtgg gtgaaatcct
      301 tcaggggatc aaagccggga ttggggctga ccgccggcgc aatcacgttg taggcgatgc
      361 tcgacgttgc tagcgtgtag ccgtcgggcg ctgcttgcgc cgcggctgcc atcccgatca
      421 agccgccagc acccgcccgg ttctccacga agcagcgctc atgaaacgtc tcgctgaagt
      481 gatcgcagac gaggcggccg aacatgtcgg ccgcgccgcc gggcgtcgag ggtgcgatga
      541 tgcgcaccgg ccgcgtcggc cagtcggcgg cgcatgtcgc gtcggtcgag ccacaaatga
      601 cggcgaagac gagcgcggcg gcaagacttc tcatggtgtg ctcacgagga tccatgatgc
      661 gggctttgcg acgcgcccgc aagcgacggc caatatgacg gaggcaggtc cgttgcgggg
      721 gcccctgatt tccgcttagg taccggcgcc gatgtttcgc cgcatcggcg ttggtcggag
      781 atcgcgcatg gacgcggact tgctgacgga aggagatgac acgatcgagc tcaagggctc
      841 gtccattatc gccgcgatca aagcaagcgc catcgagttc atcctttcag tgcccgatat
      901 cgtaaccagc ggcggcttgt tgcggcccat ttcgcgcgat cgggcattca agctcatccg
      961 ggtgtgcaag gaggacgaat gcatcggcat cgcctccggc ctcttttatt gcgacaagcg
     1021 cgcgttgtcg ctcatccaat acacgggctt tctcgactcc atcaacgcca tccgcggcgt
     1081 cgcggtcgag tatcgccagc cgatctgcat gatggtcggg ctgctcgggc acgatccctc
     1141 gaccacgccg cgcaattcgc cccgctacgg cgtgcgcatc atcgagccga tcctcgacgc
     1201 catgggtatc ccgcaccacc tgatcaatga agacgccgac gttgcgaaaa tccgtcccgc
     1261 gatcgaggat gcctacgcga cctcgcatcc ggttgcgctc ctgatcggcc gcaggccttc
     1321 cgcgtcatga tgaagcgaga cgagtgcttc cgcgcgctgg cgcggcacgt gaccgacgag
     1381 atcgtggtcg caacctacag ttccgccgta gactggctcg cactcggcga gcgcgcgctc
     1441 aactactttt cggtgggggc gatggggctt gattcctcgc acgcgctcgg actggcgctg
     1501 ggccggccgg acaaacgcgt catctgtctg cagggcgacg gcagtctgct gatgaacctc
     1561 ggatgcctgg tgaccatcgc cgcagcggcg cccaagaacc tcctccatat ggtcgtgcag
     1621 aacggaacct acgaggcgaa cggctcccat ccgatcccga acaccaaggt cgaattcgcc
     1681 gccatggcgc gcgcggccgg ctacgcgcac gtgcatgagt tttcggagct ggccaatttc
     1741 gagcagcagg ccgcgcacgt cctcaagcag gacggcccgg tgttcgcaac gctccatgtg
     1801 gagcgatcga agccgctcac ctacgactat gcaaacctct acgatccggc acggcgcgtc
     1861 gcgctcaaag cggcgctgcg ggcagggtag tacgttgtgg tcgcaccgca cttgccgcgc
     1921 attcgcgcgg aaatcccgta gcccgcatga gcgaagcgac atgcggggcc ccggatttcg
     1981 cttcgctcaa tctgtcaagc gcgttttagc tagcatcgtc agaggcctac ggcaacaccg
     2041 cccagcgcca tgctaacgct gcgctaacat cccgtttccc ggctacactg cctcatgccg
     2101 aggacaccga gaaacgcgaa gaagcgcaag aagcctgacg cccgctacga cgctttccgc
     2161 gacgaatggg agcggcggcg caaagggccg gatcgaggcg gcggcgattg gctcgtcgag
     2221 aggccggacc tgccggtgga cccgctgccg tttctccaaa cactgcggcg gctcttctac
     2281 ttcacccagg atagtcgctt catcgagttg catcgacacc tgataggaag cgatttgatc
     2341 gaccccgtca ccgaaaagtg gtcccttttc ggcaccaagc taacgcggac tctcttcgaa
     2401 ctcatcgagg aggaaatccg ggcgggcacc accgagcgtc aggccatcgc cgacgcggtc
     2461 gcggcgttcg tactctttcc tgacgcgcag agttttgacg ccgcgtggaa acacttggag
     2521 cgtcggttac gcgaatggcg gaaggcgtta gacaaaaacc agcctaaaaa agtctaatgc
     2581 ggatcgtcgc aaactgtatc aagtaactgt tcgtctcgtc cacgaggagg cgaacaacaa
     2641 tgtctgaccg aacgttggtc aagctcggcc gccgctatcc cggaaccagc cgaatgacgc
     2701 ggtggcgctg gcggcgcgac cctgatttcc ccgacccggt cgtcatcaac ggcaccgaat
     2761 actatgattc ggatgagttg accgcatatg aagaagcgcg ccgccgtcgc cgcccgcaga
     2821 aatcttcccc ctgattacac gaacgccgct cccggttgga gcggcgttcg caatcttacc
     2881 gacgctggac ggcgtcggca tcggcagcgc gattgccaat aacaccgtcc cgcgtcggcc
     2941 cgcaaggacc acgacgagga cggccatgcc gaccaatctc actgaaaccg aatcacgcga
     3001 ctggcaatgc cacgcgaagc ggatcgccgc cgcatggcag aagggcgtcg agagcatcat
     3061 cgaaaactca cgcgcggcgg cactcgcaat cgcgggtgtc gccatttctt cgaggcaact
     3121 cccgtgcgcg acatttatcg aaaagtctca gtgcaggagg ttcgttatgc gtgagttacc
     3181 gcagactcga ccgctcgccg cacatatctg ggcgaaggat ccagacggct tctatgttga
     3241 acctgagtgg gtcgacgagc ggctatttga cgtcgaatcc ttcgctggca tcatcatgga
     3301 tccggcctgc ggcatcggcc acatccccga cgctgcccgt gctgcaggct atcaaaccat
     3361 cgcaatcgat ctcgtcgatc gcggttacga gcacttcgac ggagtcgcgg attttctttg
     3421 ccgcgaccgg cgcgtcgcaa atatcgtctg caatccgcct tactacatct gccgcaactt
     3481 cgctctccac gcgctgaggt tggcgtaccg gaaggtggcg atgatctggt tgttgcgcag
     3541 attgaacgca gcgacctggt tgcgagctac gccacttcgc gcggtctatc tcctgagtcc
     3601 gcgcccgagc atgccaccgg cgcaagtcat ccttgccggt gaaaagcctg gaggcggccg
     3661 tcaggacttt tgttggctcg tttgggagcg cggttacatc ggcgcgccgg aactgcattg
     3721 gctttgtcgc gacggagatg accatgcggc atgagtgacc gcttcgctat ccggcgcgcg
     3781 cgcagttatg acggcggccc tgccgcaaag catcttgcgc agatcgatgc tccttcgatt
     3841 gccgccactg ttctgaaagc cgccgcatat ctggacgcgc tccgccacgg gagcacatca
     3901 tgagcgacct gccggcaaag taccactttc acgaattgaa acgactgctc ggcgattaca
     3961 aactcaaggt ctacatgccg tgctttcact gctgggagcc cgaagcggca gagatattat
     4021 ttgcgtttgc taagtttggg ggagcggctc acgaacaatt gccgtccggc acgctgccca
     4081 aaatggacgc tcttttcgct cccgatcctg agcccgaaaa tctttttatg ctccgggact
     4141 acgtcttgag cggcgacacg atcgtggctg cggcaattaa agcagcaggc ggaattcgtt
     4201 tactggcccg caagctcggc attcgccccc acgccatcga acagtggaag tgcattccta
     4261 ctgaacagtt gcaagcaatc gcaagaatca ctggcctgcc gcagcacgtt ctgcgccccg
     4321 atttgttcga tgtgtccgaa ccgcaattct attgcacaga ggaggaagca gacgagattt
     4381 ggaagtggag ccacgcctgg cagctctggc tgccgttgcc atacccaccg ggattccttc
     4441 cagcttgctg ctgcgcgcgc cgaccgcgac taacgctcgt cgtggacagg ggcgccgcat
     4501 gaaccccggc ccatgggaag gatttccccg gcttgtgatc gagtacgcga tcacacgtaa
     4561 ccgacagacg ccgctggaag gtgattggaa ttacggttgg acgtgtcatc cagagccgcc
     4621 gcaatctgat gccggctg
//
LOCUS       DATLJT010000122         2640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2073541,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000122 DATLJT010000000
VERSION     DATLJT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073541"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VM910_02680"
     CDS             <1..745
                     /locus_tag="VM910_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI61491.1"
                     /translation="EHRATQGDSDEQRITDLVVASRMLVQEGVLDSFGHVTVRSLKNP
                     NHYFMPRAMPPALVTTNDIVEFDLDSVPIDANAPRTNGERFIHGEIYRVRPDVQAIVH
                     SHSPAVIPFSISPDRPLRPVLHMAGFLPARVSVFDHRDVSKDDPSLRGKLMINNAKLG
                     AALAKTLGNDSVVLIRGHGNAVVGASVPWAVLRAVYTQLNARVQMEAIALGGQVIYLN
                     EVELKMHPVEVFDVDRPWQNLKSRLPKNP"
     gene            763..1308
                     /locus_tag="VM910_02685"
     CDS             763..1308
                     /locus_tag="VM910_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61492.1"
                     /translation="MTRIAILDRADMNAEQARVYDAAKQTTGIVGGPYHAYIRLPKVF
                     EACQHLRATLAAGPLSRREQQIVNLAVARHWNARYPWYAQARNSLAVGIEQPVIDAIN
                     ARKTPNLADARERTCFAVAKEILADKGLSDDSYAAAEKVMGLEGLVALVAAVGSFSMT
                     CLTAATFQIDPPPNNPTPLAQ"
     gene            complement(2225..2619)
                     /locus_tag="VM910_02690"
                     /pseudo
     CDS             complement(2225..2619)
                     /locus_tag="VM910_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
BASE COUNT          511 a          807 c          851 g          471 t
ORIGIN      
        1 ggagcaccga gcgacgcaag gcgattccga cgagcagcgc attaccgatc ttgtcgtggc
       61 gagccgaatg cttgtccaag agggcgtact ggacagcttt ggacatgtga ccgttcgctc
      121 gctgaaaaac ccgaaccact atttcatgcc gcgggctatg ccgcctgcgc tcgtgaccac
      181 aaacgacatc gtcgagttcg atctcgatag cgtaccgatt gacgccaacg cgccgcggac
      241 gaacggtgag cgattcattc acggagagat ctatcgagtt cgccccgacg ttcaggctat
      301 cgtgcatagc cactcgccgg ccgtgattcc gttcagcatt tcacctgatc gtccgctgcg
      361 ccccgttttg catatggccg gatttcttcc ggcccgagta tcggtgttcg atcaccgtga
      421 tgtttcaaag gacgatccaa gcctccgcgg caagctgatg atcaacaatg ccaagctcgg
      481 cgctgcgctg gcgaagacat tgggaaacga ttccgtcgtt cttatccgcg gacatggcaa
      541 tgccgtggtt ggagcctctg tgccatgggc ggtgttgagg gcggtctata cgcagctcaa
      601 cgcacgggtg cagatggaag cgatcgcgct tggcggtcag gtgatctatc tcaatgaagt
      661 cgaactcaaa atgcatccgg tcgaagtctt cgacgtggat cgaccctggc agaacctgaa
      721 aagtcggctt ccgaaaaatc cctgacctgc aggagatatc gcatgacgcg cattgcaatc
      781 ctcgaccgcg ccgacatgaa cgccgagcag gcgcgcgtct acgacgccgc caagcagacg
      841 accggcatcg tgggcgggcc gtaccacgcc tatatccgcc tgccgaaggt gttcgaggcc
      901 tgccagcacc tgcgcgccac gctggcggcc gggccgctca gtcgccgcga gcagcagatc
      961 gtcaacctcg ccgtcgcccg ccactggaac gcgcgctatc cctggtacgc ccaggcgcgc
     1021 aactcgctcg ccgtcggcat cgagcagccg gtcatcgacg cgatcaacgc gcgcaagacc
     1081 ccgaacttgg cggatgcgcg cgagcgcacg tgctttgccg tggcgaagga aatcctcgcc
     1141 gacaagggtc tgagcgacga cagctacgcc gccgcggaaa aggtgatggg gctcgagggc
     1201 ctggtggcgc tggtcgcagc ggtcggcagt ttttcgatga cgtgtctcac cgccgcgacg
     1261 tttcaaatcg atccgccgcc gaacaatccg acgccgctcg cgcaatagct cgtcgccctt
     1321 agaccgccgc gacgtgagca cgaacgccgc gcggcttctt catcttaatc gccgctcccg
     1381 agcgggggga tcggcagcca gaacaggagt cgttgacagc gatcgaccaa aaaaagaccc
     1441 ggcaggatga ggcctgccgg gtgtctctgg cgtcgctcac cgtctgggag gcaacggctg
     1501 cggattgagg gccgcgctcc gctgcggggc cgggcaatcc ttcaggaagc cgtaaccgcg
     1561 attgatgtcg acatcctgta cgcactgact gccgcggcga accggaccca cactcgcgta
     1621 ccattcccat gggccggagc gttgaccggc ggatgcttgc ggggagacgc tatagggcgg
     1681 cggaccagag ccagggctgt agacggtcgc agcgctgtag gtttgacggt cagatcgacg
     1741 gtaggcgcga ggggtatagc gtggcgacgg ggcataggcc ggcgcagggc tataggctgg
     1801 agcagggcta taggctggag cagggctata ggctggagga gcgctatagg ctggaggagg
     1861 cggagagccg tactgggcgt ttgccgtctc tgacccccgc ggtcgttgcc aaaatgccga
     1921 cgccggcgat cgcgatcaga gcgatgatta acctcccttt gcaccaacac gtgtgaggca
     1981 aaaaacgccc gcgtgaggcg aatgttccga ctgtggcggc aaagaaaaca cttcaaagcg
     2041 aagagcttgc agcgcgcggc aacgcgcggc ggcgtgtcat tcgctaacga aaaccccgcc
     2101 cgggtgtgtg gcgggaaggg aagttgcttc ccgggcgggg tctcgggaac ccttgtgggc
     2161 tggatttatg agagcgggct ccgctcaggc aaacacgggg gcgaggtcat tattcctgat
     2221 cgcctcataa aagaagctgc gtttgcatca cgaccgcaac gagcctcccc tcggctgtcg
     2281 taacgcgcgt ttgccacacc attgtgcgcg ctccgcggtg gaccggtgtc gtctcgccga
     2341 ccacgcggct gccgaccgtt gccgcggcga tgaagttggt tttgctttcg atcgtggtcg
     2401 tgcccgctca tccttcaaat tcgcgatcgt cgcgagcgcc ccgagcgtat cggcaaaggc
     2461 catgatggcg ccaccatgca gaacggccgg gcgggtacag aactcgggcc gaacggtcat
     2521 ttcgccgacg atcttctccg gctcggcgct gcgaagatcg atgccgagaa gcgcggcgaa
     2581 aggcagtggc tgcgccttca gtcgtgccag caggtccatt catcgcctcc gatgtcgtag
//
LOCUS       DATLJT010000123         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2763089,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000123 DATLJT010000000
VERSION     DATLJT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763089"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_02695"
     CDS             complement(<1..230)
                     /locus_tag="VM910_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVI61493.1"
                     /translation="MLVTSCAVGPDFLHPTPPEITRYTREPLTHTSSTDVASGRRQVF
                     APGGDIPEQWWALFRSPRLNALIEQALHNNQSL"
     gene            580..1809
                     /locus_tag="VM910_02700"
     CDS             580..1809
                     /locus_tag="VM910_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI61494.1"
                     /translation="MAAGPTNSRVSAAIAAALFVLVIVLYWASHFGGAPKGGPGGPGT
                     GGSHPDAPSAAAGEQQASIDLSDAQLSAVKVEPVEERDFPRDKEAVGSIDFNEELSVQ
                     VFTPYAGRIIGLFASVGDDVNKGQTLFTIDSPDLLQAESTLIAAAGVLQLTTRNLARL
                     KELYTTRAVSQRDLDQGTSDQQTAEGNLRAGRDTVRLFGKTDAEIDHIIADRKADPTL
                     VVPCPIDGRVTARNAAPGLYVQPGSAPAPFTVTNVDTMWMLANVAENDSPAFQVGQAA
                     QVRISAFPGRLFDGTITTVGSIVDPTTRRVLVRSEVKNPQHELRSGMFANFVISVGAP
                     VRSPAVALNGVVRQGDGTQTIWVTADRRRFTKRTVEIGQQRDGYRQILQGVQPGELVA
                     TDGAIFLSNMLATASAR"
     gene            1839..>2153
                     /locus_tag="VM910_02705"
     CDS             1839..>2153
                     /locus_tag="VM910_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI61495.1"
                     /translation="MLRSIVAFGLTRRAIMLLGLLVFIAGGLVAFTRLNIEAYPNPAP
                     VILEITAQAAGLSAEEMERYYTIPMEVGLYVTPGVENIRSTSFYGLSFVRVTFKYGID
                     YFF"
BASE COUNT          408 a          680 c          635 g          430 t
ORIGIN      
        1 agactctgat tgttgtgcaa cgcctgttcg atcaacgcgt tgagccgcgg tgatctgaat
       61 agcgcccacc attgctccgg aatgtccccg ccgggcgcga atacctgccg ccggcccgaa
      121 gcgacgtcgg tcgatgacgt atgcgtcaac ggctcgcgcg tataacgggt gatttccggc
      181 ggcgtcgggt gaaggaaatc ggggccgacc gcgcagctcg tcacaagcat cgcggccgcg
      241 agagcggtgg tatttgcaag ccacctggcc gctacacgag acatcaggca atagcactat
      301 tgaaaccggt tacgacaggg cacccccctg ccattatttc cgatttcgcc accgatggct
      361 gtgcttgctt ggccacagtg cacatgtttc acgccgggcc acgccgggat ttgatgccga
      421 agcgctgggt atccccggcc ttgataaact gtcacgaatc gtaaggcata gtgcgattaa
      481 tccagtcccc ctgtgattaa tgacagtaga ccgcatcggc tgctgatggc gttgtcgccg
      541 cagttcctgc agttccggaa cgtttgagta gagccgtcca tggcagctgg cccgaccaat
      601 agtcgcgtga gtgctgcgat tgccgccgcg ctgttcgtgc tcgtcatcgt gctctactgg
      661 gcttcgcatt tcgggggtgc ccccaagggt ggaccgggtg gaccaggcac aggcggctcc
      721 catcccgatg cgccgagcgc cgccgccggt gagcaacaag ccagcatcga cctttcggat
      781 gctcaactct cggcggtgaa agtcgagccg gtggaggagc gcgattttcc ccgcgacaag
      841 gaagcggtcg gcagcatcga ctttaacgaa gagttgtcgg tgcaggtgtt cacgccgtat
      901 gccggacgga tcatcggcct atttgcctcg gttggtgacg acgtcaataa aggtcagacg
      961 cttttcacca tcgacagtcc cgatttgctg caggccgagt cgaccctgat cgccgcagcg
     1021 ggcgttttgc agttgaccac gcgcaatttg gcgcgcctca aggaactgta cacgacgcgc
     1081 gccgtttccc agcgcgattt ggatcaaggc acttcggatc agcagactgc agaaggcaat
     1141 ctgcgcgctg ggcgcgacac cgtgcggctg ttcggcaaaa ccgacgccga gatcgaccat
     1201 atcatcgccg accgcaaagc agacccgacg ctcgttgtgc cgtgcccgat tgacggtcgc
     1261 gtcaccgcgc gcaatgcagc gccgggactc tacgtgcagc caggaagcgc gcctgcgcca
     1321 ttcaccgtaa cgaatgtcga tacgatgtgg atgctggcaa acgtcgctga aaacgatagt
     1381 ccggctttcc aggtcggcca ggcggcgcaa gtcaggatca gcgctttccc gggccggctg
     1441 ttcgacggca cgatcacgac tgtcggctca atcgtcgatc ccactacccg ccgcgtcctc
     1501 gtccgctccg aggtcaagaa cccgcagcac gaactgcgtt cgggcatgtt cgcaaatttt
     1561 gtcatcagcg ttggcgcgcc ggtgcgctct cctgcagttg ctctcaacgg cgtggttcgg
     1621 caaggcgacg gtacacaaac gatctgggtc accgccgacc gccggcgttt caccaaacga
     1681 accgtcgaga tcgggcagca gcgcgacggc tacaggcaaa tccttcaagg cgtgcagcct
     1741 ggcgaactcg tcgccaccga cggcgcgatc tttctcagca acatgctggc caccgcaagc
     1801 gcgcgatgag cccactacga gcaacatcga ggtgagcggt gctgaggagc attgtcgcat
     1861 ttggcttgac ccggcgcgcg atcatgctgc tgggtctgct cgtttttatt gccggcggtc
     1921 tcgttgcctt tacccgcctt aatatcgagg cctatccgaa tcccgcgccg gtcattcttg
     1981 aaatcaccgc ccaagccgcc ggcctgtccg ccgaagagat ggagcgctac tacacaatcc
     2041 ccatggaggt cgggctctac gtcacccctg gcgtcgagaa catccgctcc acctcgtttt
     2101 acggcctgtc ctttgtccgc gtcactttca aatacggcat cgactatttc ttc
//
LOCUS       DATLJT010000124         3435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2533033,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000124 DATLJT010000000
VERSION     DATLJT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533033"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VM910_02710"
     CDS             complement(<1..945)
                     /locus_tag="VM910_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI61496.1"
                     /translation="MLEAIGNVPQRWVGSPVERKEDQALLTGQARFIDDLSPIPGIRF
                     AAILRSPHPHARIGRIDLARARELPGVRGIVTGKDVSDLIGPVPSVVKAPLAYYPIAI
                     DRVRYVGEPVAVVIADTRYIAEDACDLIGVEYDVLPAASDLRSATARDAPVIHERVGS
                     NIISRRSFSYGNPDAAFAGADRVFEFSYSYPRYASTPMETFGVIAQFERAPDRYTVWS
                     NFQGPFVVQPLMASALRVPGHRLRLITPPSSGGSFGVKQAVLSYIVLLAAVSRKTGVP
                     VKWIEDRAEHLTASSASSDRVGTVEAAFTKDGELTGLRF"
     gene            1265..1897
                     /locus_tag="VM910_02715"
     CDS             1265..1897
                     /locus_tag="VM910_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HVI61497.1"
                     /translation="MTVDPSLQSAIDALSFPGIVIGHRLISPGDEDALLPEEAAAFAS
                     SVVKVRRASGSARIVARQLLKQLGQPECALPRTSGGAPAWPPGIVGSLAHDSRVAVAA
                     IALGFDVGAVGIDVEPAESLPPELLDLVATPQERLRIGDDPYQGRLLFVAKEAVYKAV
                     YPLDQTFLDHHDVQIRFAEHKAVVRNGRIVELRFCIATHLVALAVLPGLR"
     gene            complement(2157..>3435)
                     /locus_tag="VM910_02720"
     CDS             complement(2157..>3435)
                     /locus_tag="VM910_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVI61498.1"
                     /translation="GEQVMKAYLWSDGEPPPTTTRGRWRRFVERHLPSLVIYLMVATL
                     VGIVLYPHMVVTVPSGYVGVLWKRFGGGTVLDPRRLKNEGFNLILPWNQVFLYDLRLQ
                     SFTESYNAISSDGVSLTATIIVRFRLQRDAVPVLHQAIGPNYEQVLVQPGIGSLTREV
                     IAQYTAEQVYSTARQEIQDKIRSLVEDRLSEKMMEHEGEESYRVSMRDTIILYDILVT
                     GIELPAAIVAAINRKTEQYYIAEEYKFRVEREKRESERKKIEAEGIRDFQQTVSQGIS
                     ESYLRWRGIEATLQLSQSTNAKVVVIGSGKDGLPIILGNVDTAAAPAGSTPASGNDSA
                     TKESTTAAGPTVPLEKTPAAGLATPSEKIPPITSGAPSATPSEGSRSFWPFSLSDIET
                     YLYQIVRPEPKTESKAEPRSRQPSDRPLTAQPQ"
BASE COUNT          649 a         1028 c         1039 g          719 t
ORIGIN      
        1 gaatcgcaat ccagtgagct cgccgtcctt cgtgaacgcc gcctcgactg ttcccacgcg
       61 atcgcttgag gcggacgagg ccgtgaggtg ctcggcgcga tcttcgatcc acttgacggg
      121 cacgcccgtc ttgcggctga ccgcggcgag aaggacgatg tacgaaagaa cggcctgttt
      181 gacaccgaaa cttccgccgc tgctcggcgg cgtgatgagg cgaagtcggt ggccgggcac
      241 gcgcaatgcg ctggccatga gcggctggac cacgaacggc ccctggaagt tcgaccacac
      301 cgtgtaacgg tcgggggccc gttcgaattg cgcgatgacg ccgaacgtct ccatcggcgt
      361 agaggcgtag cgcggatacg agtacgagaa ctcgaacact cgatcggccc cggcaaatgc
      421 agcatccggg ttgccgtagc tgaagctgcg ccggcttatg atgttcgaac cgactctctc
      481 atggatcacc ggggcgtcgc gcgcggtggc acttcgcagg tcggacgctg ccggaagcac
      541 gtcatattcg acgccgatca ggtcgcacgc gtcttccgcg atgtaacgcg tgtccgcaat
      601 caccacggca acgggctcgc cgacgtatcg cacccggtcg attgcgatgg gatagtaggc
      661 gagcggagcc ttcacgacgc ttggcaccgg tccaatcaga tcgctgacgt ccttgccagt
      721 gacgatgccc cggacccctg gcagctcccg cgctcgcgcg agatcgatcc gtccgatccg
      781 cgcgtgcgga tgtggcgaac gcaagatcgc cgcgaagcgg attcccggaa ttggcgagag
      841 atcgtcaata aagcgcgcct gtccggtcag gagcgcctga tcttccttgc gctcaacggg
      901 cgagccaacc catcgctgcg gcacgttccc gattgcttcc agcaaaacgc gatccacctt
      961 ggaaaaccgt tgacctcgcc cttttttggc cgtttcgtct cgttgcatga tctataggac
     1021 cagccgggtc gtcgcgacaa gattgacaat gggcaaaatc atttgccgtt atagaggcgc
     1081 accgagccgc aggccggcgg tcgaggctgc aagatcaaca tcagttacct gcgctgggaa
     1141 tagtatcacg acagcgccat gggagcggtt gatgcatgag gaattcttga gccgagtggt
     1201 gcgtgtgctc tggcgcccaa atacgcacga cccacgaggc cgataactcc tgccgagaat
     1261 gattatgacg gtcgatccct cccttcagtc agcaatcgat gccctctcat ttccaggcat
     1321 cgtgatcggc catcgcttga tctcacctgg cgatgaagat gcgttattgc cggaggaggc
     1381 tgctgccttc gcctcgagtg tcgtcaaggt gcggcgtgcg agcggctcgg ctcgcattgt
     1441 cgcacgtcaa ctcctcaagc aattggggca gcccgaatgc gcgctaccga gaacttccgg
     1501 gggagcgcca gcctggcctc caggaatcgt cggctcgctc gcccacgatt ctcgtgttgc
     1561 ggttgcagca atcgcattgg gttttgatgt gggcgcggtg gggatagatg tcgagccggc
     1621 cgaatcgttg ccgcctgaat tgctggactt ggtcgcaacg ccgcaggagc ggttgagaat
     1681 aggcgatgac ccttaccagg gacgactgct tttcgtcgcc aaggaggccg tttacaaggc
     1741 agtataccca ctcgaccaaa cgtttcttga ccatcacgac gtgcaaattc gctttgccga
     1801 acacaaagct gtcgtgcgca atggccggat cgtcgaattg cgattttgca ttgctacaca
     1861 cttggtcgca ttggccgttc tgcctggcct tcgctgaggc tcgacctaaa gccaatgatt
     1921 ttcgacctgg gatcgcattg ttatcggcgt ggggttcacc gacttccagc gcgagacgcg
     1981 tggcattcgt cggcgcttat actataaccc gcagcggggc ggcgtcgccg aaaagggggc
     2041 gacgcgggtt gagaagcacg agggaggact cgtcatgtct cagattcgtc tcacaaccat
     2101 ggcgggatcg agactgccat gcgatctcgg ctttgccgat cgcgcagggc cgcccgctac
     2161 tgcggctgcg ctgtgagcgg tcggtcggac ggctgccttg agcgaggctc cgccttcgac
     2221 tctgtcttcg gttcaggacg tacgatctgg taaagataag tctcgatatc ggagagactg
     2281 aacggccaaa aggagcgcga tccctcagag ggagtcgcag acggagcgcc cgaagtgatg
     2341 ggcggtatct tctcgctcgg ggtcgccaat ccggcagcgg gcgttttctc caacggcacc
     2401 gtcgggccgg cagcggtcgt gctctccttg gtcgccgagt cgttcccgct cgccggcgtg
     2461 ctccccgcgg gcgccgctgc ggtatccaca ttgccaagga tgatcggcaa gccgtctttg
     2521 ccgctgccga tgacgacgac tttcgcattc gtggattgcg aaagctgcag cgtcgcctcg
     2581 atgccgcgcc aacgcaggta ggactccgag attccctggc tgacagtctg ttgaaaatcg
     2641 cgtatgcctt ctgcctcgat cttcttgcgt tccgactctc gtttttcgcg ttcgacgcga
     2701 aacttgtatt cctccgcaat gtagtattgt tctgttttgc gattgatggc ggcgacgatg
     2761 gctgcgggca gctcgatgcc ggtcacaagt atatcgtaga ggatgatggt atctctcatc
     2821 gagactcggt aggactcttc tccttcgtgc tccatcatct tctcggatag tctgtcctcc
     2881 acaaggctgc ggattttgtc ctggatctcc tggcgtgccg tcgaatagac ctgttcggcc
     2941 gtatattgag caatcacctc gcgtgtcaga ctgccgattc cgggctgcac cagcacttgc
     3001 tcgtaatttg gaccgatcgc ttggtgcaac accggcaccg catcgcgctg caacctgaac
     3061 cggacaatga tggtcgcagt caggctcacg ccgtcgctgg agatggcgtt gtaggactcg
     3121 gtgaaggatt ggagcctgag gtcatacaga aatacttgat tccagggcaa aatgagattg
     3181 aaaccctcat tcttcaaccg ccgcgggtcg agcacagtgc cgccgccaaa gcgtttccac
     3241 aggacgccga catagccgct gggaaccgtt accaccatgt gagggtagag tacgatcccc
     3301 acgagtgtcg cgaccatcaa gtagatcacg aggctcggga gatggcgttc aacgaaccgc
     3361 cgccacctac ctcgtgtcgt cgtcgggggc ggctccccat ctgaccacag atacgctttc
     3421 atcacctgtt ctcca
//
LOCUS       DATLJT010000125         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4141505,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000125 DATLJT010000000
VERSION     DATLJT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141505"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VM910_02725"
     CDS             <1..310
                     /locus_tag="VM910_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HVI61499.1"
                     /translation="DGRVGKGSGVVLFRNGKIFGGDAFLFYIGTYTAKDNTFKGEVLV
                     QRHTPSPDVDPLFGGPSPVGIGVTGTFTDTRGEMNGTALIGKASQIFGATLRKLADID
                     "
     gene            complement(320..2887)
                     /locus_tag="VM910_02730"
     CDS             complement(320..2887)
                     /locus_tag="VM910_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI61500.1"
                     /translation="MASVIAIRHIDSTFVELRRLQDVGDVAEQIDRRVNELRLAARDF
                     VTDPNAQPDRVVGVASELGALLKKTRLELAPEQQDMIDGVTQRLANYRDGIERVTALI
                     ARRAELLAALPPVRARFEQAVTAAADQSGAKTLLRAQDDIAVALLANDPGGAEQAAMR
                     MRALTIDDPALRGAADAFADAITAIAGTESEIAKLDKEVLGIEGRLIGRVTELLRELS
                     ARRGRVLSGDFARTLTEAKWQSIILSTIGVLIGTFAASFVVRRTVGPLTSIASAIRAL
                     AAGKKDTSIPATEVNNEIGDIARAAEVFRRTLVDADAAREAAVRALAEQRLAEESYRK
                     LFETSIDGIYVTTPGGKLLNANPALARIMGYDRPEQLIESIDDVAQNVYVNSAAREEF
                     QRLMQRDGMVREFEYQVRQRGGGILWLSDSATAVRDEKGEVTRYEGTVRDITDQKRAE
                     DAIAEGRRLLQQVIDTVPAVINVKDNQLRYVLMNRYMSGIFGIEPEDAIGRTTTELMS
                     RYGAQKTDEFDKRVLEGGMQLGFYEDEYVDSKGQMRQWLVNKLPLLDTDGKVDKIVSV
                     ALDIGERKRSELEMRKARDAAETALRNLRDTQASLIEAEKLAALGRLVAGVAHEVNNP
                     VGISLTVASALDRKTANFAAEVERGDLRRSSLNEFLGTSRDASAQLVANLNRAAELIQ
                     SFKQVAADRNYSDQRTFDLADLTEQVVLSLRPGLRKRNLMLNVECQPNLVMTSYPGPY
                     GQVLTNLFLNAVAHAFPDGRSGVVDIKVRESGKDNVEIIFSDNGCGMSLDVRRRAFDP
                     FFTTRRDQGGTGLGLHIVYSIVTNRLGGRLDLDSEPGGGTRIQIILPRVAPLEQAAE"
BASE COUNT          496 a          926 c          924 g          548 t
ORIGIN      
        1 ggatgggcgc gtcggcaaag gcagcggcgt ggttttgttt cgcaacggca agattttcgg
       61 cggcgacgcc tttctgtttt acatcggcac ttacacggcg aaggacaaca ccttcaaggg
      121 cgaggttctc gttcagcgcc ataccccgag ccccgacgtc gatccgttgt tcggcggccc
      181 gagcccggtc ggtatcggcg tcaccggcac cttcaccgac acgcgcggcg agatgaacgg
      241 aaccgcgctg atcggcaagg ccagccagat ctttggtgcc acgctgcgca agcttgccga
      301 catcgactag caagaggcgc tactccgcgg cttgctcgag cggcgcgacg cgcggcagga
      361 taatctgaat tcgcgtgccg ccgcccggct ccgaatcgag atcgagccgg ccgccgagac
      421 ggttggtgac gatgctgtag acgatgtgca gaccgagccc ggtgccgccc tgatccctgc
      481 gcgttgtgaa aaaaggatcg aacgcgcggc gccgcacgtc gagcgacatg ccgcagccgt
      541 tgtcggagaa gatgatctcg acattgtctt tgccggactc ccgcaccttg atgtcgacga
      601 cgccggaccg gccgtccgga aaggcatgcg ccaccgcgtt gaggaacaga ttggtcagca
      661 cctgcccgta tgggccggga tagctggtca taacgagatt gggctggcac tcgacgttga
      721 gcatcaggtt gcgcttgcgc aagccaggcc gcagactgag gaccacctgt tcggtgagat
      781 cggcaagatc gaaggttcgc tgatccgaat agttgcggtc ggcggcgacc tgcttgaacg
      841 actggatcag ttcggccgcc cggttgaggt tggccacgag ctgagcggag gcatcgcggc
      901 ttgtgccgag aaactcgttg aggctcgaac gacggagatc gccgcgctcg acctcggcgg
      961 cgaaattcgc agtcttgcgg tccagcgcag acgcaaccgt aaggctgatc ccgacgggat
     1021 tgttcacttc atgggcgacg cccgccacca acctgccgag cgcagcaagc ttctccgctt
     1081 cgatcagcga ggcctgagtg tcgcgcagat tgcggagcgc ggtctcggcg gcgtcgcgcg
     1141 ccttgcgcat ctcgagctcg ctgcgcttgc gctcgccgat gtcgagcgcg acggagacga
     1201 tcttgtcgac tttgccgtca gtgtcgagca atggaagctt gttgaccagc cattgccgca
     1261 tctgtccttt tgagtcgaca tattcatcct cgtagaaccc gagctgcatg cccccttcaa
     1321 gcacgcgctt gtcgaactcg tcggtctttt gcgcaccgta gcgcgacatc aattccgtcg
     1381 tcgtgcgccc gatcgcatcc tctggctcga tcccgaagat gccggacata tagcggttca
     1441 tcaggacgta gcggagctga ttgtccttga cgttgatcac cgctggcacg gtgtcgatca
     1501 cctgctgcag cagcctgcgg ccttcggcga tggcatcctc ggcgcgcttc tggtcggtga
     1561 tatcgcgtac cgttccttcg tagcgggtca cctcaccttt ctcgtcgcgt accgcggtgg
     1621 cgctgtcgga aagccacagg ataccaccgc cgcgctgacg cacctgatac tcgaactcac
     1681 gcaccatccc atcgcgctgc atcagacgct ggaattcctc gcgcgccgcc gaattgacgt
     1741 agacgttttg cgcgacgtcg tcgatgcttt cgatcaactg ctccggcctg tcgtatccca
     1801 tgatccgcgc cagcgcggga ttggcattga gcagcttgcc gcccggcgtc gtcacgtaga
     1861 tgccgtcgat cgaggtctcg aacaatttgc gatagctctc ctccgcgagg cgttgttcgg
     1921 cgagcgcgcg gaccgcggcc tcgcgcgccg catcggcgtc gaccagcgta cggcggaaca
     1981 cttctgccgc gcgcgcgata tcgccgattt cgttgttgac ctcagttgca ggaatcgagg
     2041 tgtctttctt gccggccgcg agcgcacgga tcgcgcttgc gatcgaggtc agtgggccca
     2101 ccgtgcggcg aaccacgaag gacgcggcaa aggtgccgat cagcacgccg atggtggaaa
     2161 ggatgatgct ctgccacttg gcctcggtca gggtgcgcgc aaagtcgccc gacagcacgc
     2221 ggccgcgccg cgcgctgagc tcgcgcaaca attcggtgac ccggccgatc agccggcctt
     2281 cgatcccaag cacttcctta tcgagcttgg cgatctcgct ctcagtcccg gcaatcgcgg
     2341 tgatggcgtc ggcaaaggcg tcggcggcgc cgcgcagcgc cggatcatcg atcgtcagcg
     2401 cacgcatccg catggcagcc tgctcggcgc cgcccggatc gttcgcaaga agcgcgaccg
     2461 cgatatcgtc ctgggcgcga agcagtgtct tggcgccgga ctggtcggcg gctgccgtta
     2521 ccgcctgctc gaaacgcgcg cgaaccgggg gtaacgcggc gagcagctcg gcgcgccgcg
     2581 caatcagtgc cgtgacacgt tcgatgccgt cgcgataatt ggcaaggcgc tgggttacgc
     2641 catcgatcat gtcctgttgt tcgggcgcga gctcgagccg ggtctttttc aaaagcgcac
     2701 ccaattcgga cgccaccccc acgacccggt cgggttgggc attgggatcg gtcacgaaat
     2761 cgcgcgcggc gaggcgaagc tcgttcacgc ggcggtcgat ctgttcggct acgtcgccga
     2821 catcctgcag ccgcctgagc tcgacaaagg tggaatcgat gtgacggatc gcgatgacgc
     2881 tcgccatgcc ggtt
//
LOCUS       DATLJT010000126         1981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1843804,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000126 DATLJT010000000
VERSION     DATLJT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843804"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VM910_02735"
     CDS             <1..298
                     /locus_tag="VM910_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61501.1"
                     /translation="LFCCMEMLQIDRARLMRDEPLLYRELQGVCTLCPNRQECSIDLA
                     AGEFDAAKWEEWWLYCPNSAMLRTIGALQKCGPSPRYATADDFSHLRQSDRVSI"
     gene            338..742
                     /locus_tag="VM910_02740"
     CDS             338..742
                     /locus_tag="VM910_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HVI61502.1"
                     /translation="MKRARFLCFFLAVAGVSVTAATLLPASVRDACAQRAKLTFSEDI
                     APIFKGYCESCHQPGGQGFAASGLDLRDYDGLMKGTKFGPMVVPGKPDQSNLFVLISG
                     RAKLQMPYGHKPLPSCLQDTIYSWIFEGAKNN"
     gene            complement(823..>1981)
                     /locus_tag="VM910_02745"
     CDS             complement(823..>1981)
                     /locus_tag="VM910_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61503.1"
                     /translation="SPASGRRQRIAAAVALLALPVLAGGLYLWLGSPQLASAQQERAP
                     QAAQANESVEGLISQVEGHLQRNPQDGRGWEIVAPVYMQLSRYSDAVRAWRNALQLLG
                     ESAERDANLGESLTAEANGVVTAEAKAAFVRAADLDNTLVTAQYYLGLAAEQDGDRAK
                     AAKIWRDLIAGAPAGAEWVPEVRNALARVDGSSGVPPAAAEGPIAAAPNAPNEQQSAM
                     IKGMVERLAARLKQDGSDAEGWARLVRSYRVLDEPDKALAAIADARQALAADPAKLET
                     FNRSVEQEQSTSAPTNGPNPPSRSSLAAQAVSPDHAGAGAQDMVERLAARLKTNGSDP
                     AGWLMLVRSYLTLSQKDKAIAAIDNARHALTDNPTKLEEFNEALKKFDISD"
BASE COUNT          366 a          574 c          607 g          434 t
ORIGIN      
        1 gctgttttgc tgcatggaaa tgctgcagat cgatcgcgcc aggctgatgc gcgatgagcc
       61 gcttttgtat cgcgagttgc aaggcgtatg cacattatgc ccgaaccggc aggaatgctc
      121 gatcgatctt gccgctggcg agtttgatgc cgccaaatgg gaagagtggt ggttgtattg
      181 cccgaattca gcgatgctga ggacgatcgg cgccttacaa aagtgcgggc catcgcctcg
      241 ttacgcgacc gccgatgact tcagccatct ccggcagagt gatagagtaa gcatttgacg
      301 cggtgcatca cacatagtgg ctggagggca aaacgcgatg aaacgtgcaa gatttctctg
      361 cttcttcctc gcggtcgctg gtgtgtcggt cacggcggcg acgctgttgc cggcgtcagt
      421 tcgtgacgct tgcgcacagc gtgcgaaact gactttcagc gaagacatcg cgccgatttt
      481 taaaggttat tgcgagtcct gccaccagcc tggcggccag ggctttgctg cgagcggtct
      541 cgatctgcgg gattacgacg ggttgatgaa aggcacgaag ttcgggccta tggtggttcc
      601 cggaaagcct gaccagagca atcttttcgt attgatttct ggtcgggcta aactgcagat
      661 gccttacgga cacaagccgc tcccgagctg cctgcaagac acaatttata gctggatctt
      721 cgaaggcgcg aaaaacaatt gagctgcctg aacggatcga ggctgtttgc tcgagtgccg
      781 tctgccttcg agttaacggc ctaggtgggc gctgagaaag cgctagtcag aaatatcaaa
      841 cttcttcagc gcctcgttaa attcttccag cttggtgggg ttgtcagtta gcgcgtggcg
      901 cgcgttgtca atcgccgcga tcgctttgtc cttttgacta agggtcagat acgatcgtac
      961 gagcatcagc cagcccgccg gatctgaccc gttcgtcttc aatcgagcgg caagtcgctc
     1021 gaccatgtct tgggcgcctg ctccggcatg atcgggcgag accgcttgcg ctgccaagct
     1081 tgagcgactg ggaggatttg ggccattggt cggcgcgctg gtactttgct cttgctcgac
     1141 ggaccgattg aacgtttcca gcttggcggg atcagccgcg agcgcctggc gcgcatccgc
     1201 gatggccgcg agcgctttat cgggctcgtc cagaactctg tacgaacgca ccaaacgcgc
     1261 ccagccctcg gcatctgagc catcctgttt gaggcgtgcg gcgagccgct caaccatgcc
     1321 tttaatcatt gccgattgtt gctcgtttgg cgcatttggg gcagcggcaa tcggtccttc
     1381 ggcggcggca ggcgggacgc cagagctccc gtcgacccgt gccagcgcat tgcggacctc
     1441 cggaacccac tcagcgccgg caggtgctcc ggcgataaga tcccgccaga tttttgcggc
     1501 tttggcgcga tcaccatcct gctcggctgc taatcctaag taatactggg ctgtcacgag
     1561 ggtattatcg agatcagcgg cgcggacaaa ggctgccttg gcctcggcgg taacgacacc
     1621 attagcttcc gccgtcagcg actcgccgag attggcgtcg cgttcggcgc tctcaccgag
     1681 aagctgcaaa gcattgcgcc acgcccgaac ggcatccgag tagcgactga gttgcatgta
     1741 gaccggcgcc actatctccc agccacgacc gtcctgtgga ttgcgctgca ggtggccctc
     1801 aacctgcgag atcagtcctt cgaccgattc gttggcttgc gcggcctgcg gtgctcgctc
     1861 ctgttgcgct gatgccagtt gtggggatcc aagccacaga tagaggcctc cggcgagcac
     1921 cggcagcgct agcagtgcca ctgcggcggc gatcctttga cggcgacctg acgccggact
     1981 g
//
LOCUS       DATLJT010000127         6818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2073901,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000127 DATLJT010000000
VERSION     DATLJT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073901"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VM910_02750"
     CDS             complement(<1..597)
                     /locus_tag="VM910_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI61504.1"
                     /translation="MLSGIFIDRPRLAIVIAIVITLAGVIAIFAIPIAQFPDIVPPQV
                     TLNASYPGADAEVVETTVAQPIEQQVNGIDNALYYQSASAADGSYILTVTFALGTDPD
                     INTVNVQNRASLAIPQLPAEVSRNGLTIRKKSAALLQVISFYSPNSTYDAVYLSNYAT
                     INVIDPLARIKGVGQATLFGPLDYSLRIWLDPDRLTELN"
     gene            complement(597..1718)
                     /locus_tag="VM910_02755"
     CDS             complement(597..1718)
                     /locus_tag="VM910_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI61505.1"
                     /translation="MPGRNSVAWKVVVCALSFMVSCGVAAAQGAPPPPAVSVTPVTTR
                     QVTETIDYIGRLTAVDKVDIVARVPGFLEERKFKEGQYVKKGDLLFRIEQATYKAAVE
                     EARATLAKAKATEVNAKLQLDRGKELVRNQNIPQSTVDQREADEEAAQASVMEAQAAL
                     DQAEINLGYTEIRSPIDGRIGLAIFTEGNLVQPSSGKLATIVSQDPIYVTFQVSQRNL
                     LDYFRRRAEDPGKNTQVNARIKLPNGTIYPHAGVSDFLDVQVDPTTDTVTVRATVPNA
                     EHVLIPGGVVGVTVERGAPKSALTVPQAAVLLDQAGRYVLVVDAAKKVEQRRITTGAE
                     QGRDIVVTDGLKEGEQVIVEGIQKVRPGQVVTATVVPGT"
     gene            complement(1850..2059)
                     /locus_tag="VM910_02760"
     CDS             complement(1850..2059)
                     /locus_tag="VM910_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61506.1"
                     /translation="MTIGNKRIAAIALGLALSVLASPSFAQRSENHMSAAREQALREC
                     NLAAQKYTQHTWADVQIYTDRACMA"
     gene            2299..2433
                     /locus_tag="VM910_02765"
     CDS             2299..2433
                     /locus_tag="VM910_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61507.1"
                     /translation="MSRMIQFWEGGDAKKPQVTDAAKQYFARHRAKKDQDYGYSTYIP
                     "
     gene            complement(2540..3046)
                     /locus_tag="VM910_02770"
     CDS             complement(2540..3046)
                     /locus_tag="VM910_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HVI61508.1"
                     /translation="MSFEIERKFLVRGNGWERLATRRISLRQAYLACNGKASIRIRIK
                     DDGAATLTVKSRPADLRRLELEYDIPVLEAEALMKLRQGSIIEKVRHVIPYGDLAWEV
                     DVFSGDNFGLIIAEIELRHEHQHVELPPWIGAEITGQPQYYNSSLVQRPFCSWSRQEA
                     PMPIERRA"
     gene            complement(3261..4511)
                     /locus_tag="VM910_02775"
     CDS             complement(3261..4511)
                     /locus_tag="VM910_02775"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HVI61509.1"
                     /translation="MTSEQRSSLVLAFARVLYVNGQATEQTVAAAEQLGRILGLRAKV
                     MPRWGELQLRSDDENGRVISEVAADPTGVDMDHVASAARAIEQLGAGRLAPDAAAEAI
                     AATSRAPHASTWLFALAAGAGAVGLSVIFGVEHPVPAMLIFVSAAAGAILRRGVARVS
                     ENVFLQPFCAALVAGVIGALAVRYQLSSSLRLVAVCPCMVLVPGPHVLNGALDLINGR
                     IHLGAARLVYAGLVVVAISTGLLLGLALLGVSLPVDEAGRTVPLWQDVIAAGVAVAAY
                     SVFFSTPLNMLPWPVAVGMLSHALRWVAITVLGFGAAIGTLLACLVVGLILTPVARRR
                     HMPFAAIGFASVVSMLPGVLLFRMTSGLVQIAGGAQPSLELISATIADGVSALISILA
                     MSLGLITPKLVIDALSERATQRKS"
     gene            complement(4611..6035)
                     /gene="fumC"
                     /locus_tag="VM910_02780"
     CDS             complement(4611..6035)
                     /gene="fumC"
                     /locus_tag="VM910_02780"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVI61510.1"
                     /translation="MADSRKESDSLGVVEVPADRLWGAQTQRSLEHFSIGMDLIPREM
                     ITAYAILKKAAAAANHAGKRLDDQRYKLIVQACDEILAGKHQDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCSQLAGMPLGSKAPVHPNDHVNMAQSSNDTFPSTMYIAAAVTVKQRL
                     VPAVAALRDAISAKAQAWDDIVKIGRTHMQDATPLTLGQEWSGYAGMLSDDLERIEAA
                     LHGVYRLALGGTAVGTGINSAPGFAEAAAAEIAKLTGLPFVTAPNKFTVQGSHDALVQ
                     LSGTLRTLAVSLYKIANDIRLMSCGPRAGFAELEIPENEPGSSIMPGKVNPTQCEALA
                     MLAVQVMANDVAVGFGGAGGYLEMNVYKPLMISNVMHSINLMSDGCVNFRKFLVEGTK
                     PNVKKINEYVERSLMLVTALAPVIGYDKASKIAHHAMDNDLTLKEAALQLGFVTEAEF
                     DRVVDPKKMVKPYVASEPSGKGPR"
     gene            6305..>6818
                     /locus_tag="VM910_02785"
     CDS             6305..>6818
                     /locus_tag="VM910_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HVI61511.1"
                     /translation="MSNHFTGLSLGPPLGDQRLDLCDLYTFQSPADKNRTVIILNANP
                     NADALHPDAIYRLNVDNDGDYLTDIAFSYVFSKPQNGRQTVNVFVATGSQSRSAEAIG
                     TKIIADAEVSFSAKPNIIKSGPYTFFAGSRSDAFFFDFDGIKNLFDIRGKRNFTEPHL
                     GGKSPWTGVDS"
BASE COUNT         1316 a         2104 c         2130 g         1268 t
ORIGIN      
        1 attgagctcg gtgagccggt cggggtcgag ccagatgcgc aacgaatagt cgagcggccc
       61 gaacagcgtt gcctgcccga cgcctttgat ccgcgccaat ggatcgatga cgttgatcgt
      121 cgcgtagttg ctcagataaa cggcatcgta ggtgctgttc ggcgaataga agctgatcac
      181 ctgcagcaac gcggcggatt tcttgcggat ggtgagaccg ttgcgcgaga cctccgccgg
      241 cagctgcgga atcgcgagac tggcgcggtt ctgcacgttg acggtgttga tgtccggatc
      301 ggtcccgagc gcgaacgtca cggtgaggat gtagctgcca tcggcggcgc tggcggactg
      361 ataatagagc gcgttgtcga tgccgttgac ttgctgctcg atcggttgcg ccaccgtcgt
      421 ctccaccact tcggcatcgg cccccggata agacgcgttg agcgtgacct gcggcggcac
      481 gatgtctgga aactgcgcga tgggaatcgc gaaaatggcg atgacgccgg caagcgtgat
      541 aacgatggcg atgacgatgg cgagccgcgg ccggtcgatg aaaatgccgg aaagcatcag
      601 gtccccggca cgacggttgc ggtcacgacc tggccgggac gcaccttctg aatgccctcg
      661 acaatgactt gctcgccctc cttgaggccg tcggtcacta cgatgtcgcg gccctgctcg
      721 gcaccggtcg ttatgcggcg ctgctcgacc ttcttggccg catcgaccac gagcacatag
      781 cggccggctt gatcgagcag gacggcggcc tgcggaaccg tcagcgccga tttcggcgcg
      841 ccccgctcga cggtgacgcc gacaactccg cccgggatca gcacatgctc cgcgttcggc
      901 actgtcgccc gcaccgtcac tgtgtccgtc gtcgggtcga cctgcacgtc gaggaagtca
      961 ctcacgccgg catgcgggta gatggtgccg ttgggcagct tgatgcgagc gttgacctgc
     1021 gtgttcttcc cggggtcttc agcgcggcgc ctgaagtagt cgagaagatt gcgctggctg
     1081 acctggaagg tgacgtagat ggggtcctgg ctgacaatcg ttgccagctt gccggaggag
     1141 ggctggacca ggttgccttc ggtgaagatg gcgagcccga ttcgtccatc aatgggggag
     1201 cgaatttccg tgtagccgag attgatctcc gcctggtcga gcgccgcctg cgcctccatg
     1261 acgcttgcct gcgctgcttc ctcgtctgcc tcgcgctggt cgaccgtgga ttgcgggatg
     1321 ttctgattgc gcacaagctc tttgccgcgg tcgagctgca gcttggcgtt gacttccgtc
     1381 gccttggcct tggcgagggt ggcgcgcgcc tcctcgacgg ccgccttgta ggtcgcctgc
     1441 tcgatgcgga acaacaggtc gcccttcttg acgtactgtc cctctttgaa tttccgttcc
     1501 tcgagaaagc ccggcacgcg ggcgacgata tcgaccttat cgaccgcggt gaggcgcccg
     1561 atgtaatcga tggtttccgt cacctggcgg gtcgtcaccg gcgtcaccga aaccgcgggc
     1621 ggcggggggg cgccttgggc ggcggcgacg ccgcaggaga ccatgaaact cagcgcgcaa
     1681 acgacaacct tccaagcaac ggagttacga ccgggcatcg agcttctcct gactgtggga
     1741 gcgatgttcg cttgggacgc tgccgctccg cgccagccta tacccgtgca aaaccgtcat
     1801 ccacaaacag ttccgttacc gtgacgtcgc gttattcctg ctggccatgc taggccatgc
     1861 aggcccgatc cgtgtagatc tggacgtcgg cccaggtatg ttgcgtgtac ttctgggccg
     1921 cgagattgca ctcgcgcaag gcctgctccc gcgcggcgct catgtgattc tcgctgcgtt
     1981 gcgcgaagct cggcgacgcc aggacggaaa gcgcaagccc gagagcaatt gcggctattc
     2041 gtttgtttcc aatagtcatg gttatactcc gcgattcaaa agcctatcaa ttcataagcc
     2101 tccttaataa ctggccgctt atatctactc cagcaagcgg ccagaaagcg caacgctgct
     2161 ttgcccctgg cgcatcgcct gaccgagtgc acgatcggat gacggggcag gctcgatcgg
     2221 aaacctgcgc cacagcaccc gctttcgagc gggcgggaat aagggagtat atgcacttat
     2281 ttaggcgcat atgcacgcgt gtcaaggatg attcagttct gggagggcgg agatgccaag
     2341 aagccgcagg tgaccgacgc cgccaaacaa tacttcgcgc gtcaccgggc gaaaaaagac
     2401 caggactacg gctattccac ctacattccg tgaagcgggg ataagcggcg aacgaagccg
     2461 taataccgcg gagcgccaac tcgacgaggt catcgcgcgc ccgccaaaca atgcgggccg
     2521 cacaagccta ctcactcggt cacgcgcgcc tttcgatcgg catgggcgct tcttgacgcg
     2581 accatgagca aaatggacgc tgcaccaggg agctgttgta atactgcggc tgcccggtga
     2641 tctctgcgcc gatccagggc ggcaattcga cgtgctgatg ctcgtggcgc aactcgattt
     2701 cggcgatgat caagccgaaa ttgtcgccgg agaacacatc gacttcccag gcgagatcgc
     2761 cgtaagggat gacgtggcgc accttctcga tgatggagcc ttggcggagc ttcatcagcg
     2821 cctcggcttc caataccgga atatcatact ccagctccag ccggcgcagg tccgcggggc
     2881 gagatttgac ggtgagcgtt gcggcgccgt cgtccttgat gcgaatgcgg atcgatgcct
     2941 tcccgttgca ggccaaatag gcttgtcgaa gggagatgcg acgggtggca agcctttccc
     3001 aaccgttgcc acggacgagg aattttcgct caatttcaaa actcattcgc ccccctcgac
     3061 cgggctagga ttgcaaacat gcgggctcgg gatggcgacg ggaaaaatgg attgtgacgg
     3121 cctcgtgacg ttctaatggg ctcgaatatc aaaagaaaaa agcaaaagca agcggagtgg
     3181 cgacgacgca tgcgatgggc aggagcaagg atccatcgcg tcggaattca tggctggagg
     3241 ccggcgtagt ccttcgcctg ctatgatttt ctctgcgtcg ctcgctcgct cagcgcatcg
     3301 atcacgagct tcggagtgat gaggcccaag ctcatcgcca agatactgat caaggcgctc
     3361 acgccatcgg caatggtcgc gctgatcagc tccagcgagg gctgggcgcc gcccgcgatc
     3421 tgcacaagcc cgctggtcat ccggaacagg aggacgcccg gcagcatcga caccacggag
     3481 gcgaagccga tggccgcaaa cggcatgtgc cgacggcgtg cgaccggcgt gaggatcaac
     3541 ccgaccacga gacacgcaag cagggtgccg atcgccgcgc caaagccgag cacggtgatc
     3601 gcgacccatc gcagcgcgtg ggatagcatg ccgaccgcca ccggccaggg caacatattc
     3661 agcggagtcg aaaagaagac gctgtaagca gcaacggcga cgccggcggc gatcacgtcc
     3721 tgccacaacg ggacggtcct gcccgcttca tcgaccggca gggagacgcc gagcagcgcg
     3781 aggccaagga gcaatcccgt cgagatcgcc acgacgacga gccccgcata gaccagccgc
     3841 gcggcgccga gatggatgcg gccattgatg aggtcgagcg cgccattgag gacgtgcggc
     3901 cccggcacca gcaccatgca cgggcagacc gcgaccagcc gcagcgagga actcaactga
     3961 tagcgaaccg ccagggcgcc gatgacgccg gcaacgagcg ccgcgcagaa cggttggagg
     4021 aatacgtttt cactcaccct cgccacgccg cgccgcagaa ttgcgccggc agcggcactc
     4081 acgaagatca gcatcgccgg gactgggtgc tcgacgccga agatgaccga tagccccacg
     4141 gcaccagccc cggccgcaag cgcgaaaagc cacgtcgaag cgtgcggcgc gcgcgaggtc
     4201 gcggcgatcg cctccgccgc ggcgtctggc gcaagccgcc ccgcaccgag ctgctcgatc
     4261 gcccgcgccg cggacgcgac gtgatccata tccacgccgg tgggatcggc cgccacctcc
     4321 gagatcacgc ggccattctc gtcgtcggac cgaagttgca attctcccca gcgcggcatg
     4381 accttggcgc gcaggcccag gatacggccg agttgctcgg ccgcggccac agtctgctcg
     4441 gtagcttggc cgttgacgta gagcacccgc gcgaaggcga ggacaaggct ggaacgctgc
     4501 tccgacgtca tgtcagcttc tcgcaatccg cggattcgaa ggcggcaacc tcatgggcga
     4561 gccggggccg agccaggcga gccacatcga gccccggaca tgcaagttgc tcaacgcggc
     4621 cccttgcccg atggctcgct cgccacgtaa ggcttgacca tttttttcgg atctacgacg
     4681 cgatcgaatt ccgcctccgt gacgaagccc agttgcaacg cggcttcctt cagcgtgaga
     4741 tcgttgtcca tggcgtggtg ggcgatcttc gaggccttgt cgtagccgat gacgggcgca
     4801 agcgccgtca ccagcatgag cgagcgctcg acgtattcgt tgatcttctt aacgttgggc
     4861 ttggtaccct cgaccaggaa cttgcggaag ttcacgcagc cgtcgctcat gaggttgatg
     4921 gagtgcatga cgtttgagat catcagcggc ttgtagacgt tcatctcgag gtagccgccg
     4981 gcgccgccaa agcccacggc gacatcattg gccatcacct gcacggcgag catcgcgagc
     5041 gcttcgcact gggtcggatt gaccttgccc ggcatgatcg aggagcccgg ctcgttctcc
     5101 ggaatttcca gctccgcaaa tccggcgcga ggtccgcacg acatcaggcg aatgtcgttg
     5161 gcgatcttgt agagcgacac cgccagcgtg cgaagcgttc cggacagttg caccagcgcg
     5221 tcgtgggagc cctgcacggt gaatttgttg ggcgccgtga cgaagggcag accggtgagc
     5281 ttggcgatct cggccgccgc cgcttcggca aaacccggtg ccgagttgat gcccgttccc
     5341 accgccgtac cgcccagcgc caagcggtac acgccgtgca gggcggcttc gatgcgttcc
     5401 agatcgtccg acagcatccc ggcatagccg gaccattcct ggccgagcgt cagcggtgtg
     5461 gcgtcctgca tgtgggtgcg gccgatcttg acgatgtcat cccacgcttg cgccttggcg
     5521 ctgatcgcat cgcgcaaggc ggcgaccgcg ggaacgaggc gctgcttcac ggtgacggcc
     5581 gccgcgatat acatggtcga cggaaacgta tcgttcgacg actgcgccat gttgacgtga
     5641 tcgttgggat gaaccggcgc cttgctcccg agcggcatgc cggcgagttg ggagcaacgg
     5701 ttggagatca cttcgttgac gttcatgttg aactgcgtgc cgctgcccgt catccaaacg
     5761 tgtaagggaa acatgtcctg gtgttttccg gcgaggattt cgtcgcacgc ctgaacgatc
     5821 aatttatagc gctgatcgtc gagccgcttg ccggcgtggt tggcggcggc agcggctttc
     5881 ttcaagatgg cgtaggcggt gatcatttcg cgcggaatca aatccatgcc gatgctgaaa
     5941 tgctcgagcg agcgctgggt ttgcgcgccc cacagcctgt ccgcgggcac ctcgaccacg
     6001 ccgaggctgt cgctctcttt tcgcgagtcg gccatgggat ctctcctgga ttcaatactt
     6061 aagtcgggga gaatccgggt caatcgcttc tcccgaacgg aacgcgctcc cggttaacgc
     6121 agcaccggct tctttgatgc gaaccccttg taccatccac attacatgat gcatgcgtgc
     6181 tatgcccacg gcgtcgttgc ggccgttttt gttgatggca ttttcaaagc cgacttcgtg
     6241 gatcgatgta taatccccag tccgacagac caaagctgga aatcttggtc aacaggagac
     6301 gatgatgtcg aaccatttca ccggactcag tcttggcccg ccgctgggcg atcaacggtt
     6361 agacctctgc gacctgtaca cattccagtc gcccgctgac aagaacagaa ccgtgatcat
     6421 cctgaatgca aatcccaacg cggatgcact tcatcccgac gcaatctatc gcctcaacgt
     6481 cgataacgac ggcgattacc tgacggatat cgcgttcagc tatgtgttct cgaagccgca
     6541 gaacggcagg cagaccgtta acgtattcgt agcgacgggc tcgcagtcgc gctcggccga
     6601 agcgataggg acgaagataa tcgccgacgc ggaggtctcc ttcagcgcca agccgaatat
     6661 catcaagtca gggccgtaca cttttttcgc cggcagccgc agcgacgcgt tcttcttcga
     6721 cttcgacgga atcaagaacc tgttcgatat caggggtaaa cggaacttca ccgagcctca
     6781 tctcggcggc aagtctccat ggaccggggt ggattcga
//
LOCUS       DATLJT010000128         4379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3452283,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000128 DATLJT010000000
VERSION     DATLJT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452283"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VM910_02790"
     CDS             complement(<1..1538)
                     /locus_tag="VM910_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI61512.1"
                     /translation="MARMKGAELITEYLIANGVPYVFGICGHGNVGMLDPLYQARDRI
                     KLISPRHEQVAAHMADAYFRVRHEPVATLTSCGPGSCNIVMPLACALTDSSALLAITA
                     NVPTSQFNRSPFQEVNRHYQADFVNIVRPVVKRSFQPTRVDMLPLALRQAMTAMLSGR
                     PGPVNVDVPFNVFQEEDEVDLPPVTPPLNAQRSGASPEEVSRALDMVLNAERPVIFIG
                     HGVALSEASGELTEFAKKLAIPVISSPNGMGCIDMTDPLSLGFIGRNGAFPANEAGRR
                     ADLVLAIGARFDDRSASSWLPGYSWNFPVTKLIHVDVDAAELGRNYPPDLPIAADART
                     FLRQLLTELERRSGNGDEAARRQWLAQIAQWRAAWDEFVRPNFATHATPMRPERVVSD
                     CQAVIPTDGIICCDVGVSHNWYMQFWNARRPQSMLNSWGFSGMGFGAAGALGAKLAAP
                     DRAVVAVTGDGCFTMVPHVLCTAVEYNIPVVWVIWNNFGWVSIRDIQLGMFGGREHGT
                     MFHQG"
     gene            complement(1616..2374)
                     /locus_tag="VM910_02795"
     CDS             complement(1616..2374)
                     /locus_tag="VM910_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI61513.1"
                     /translation="MVKGKSASGQSLRRAGAGQRATHRSLKEQLILAGKVLVAEGQDD
                     FTRGHISFRLPDDPNLFFMKAHSIGLDEITMPNILTIDLEGAVEAGTARRHSEVYIHS
                     EIFKARPDVNCIIHTHPTYSIALSATGRGLKCYSQPGALFYQALGSYTDTINLIRSHA
                     MGAGVARALGNGRAVLLKNHGVVVTGATIAEAVIGTIMLENGAIVQLLVEAAGDPAPE
                     FSRADIEKLKADISQPEQFTINFDYLVRRIKRRG"
     gene            complement(2480..2683)
                     /locus_tag="VM910_02800"
     CDS             complement(2480..2683)
                     /locus_tag="VM910_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVI61514.1"
                     /translation="MDWNRVEGNWKEVKGKIKQQWGQLTDNDLAQINGRRDQLEGKIQ
                     QRYGRAKDRVRKDVDDWLDTQPQ"
     gene            3234..3722
                     /locus_tag="VM910_02805"
     CDS             3234..3722
                     /locus_tag="VM910_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HVI61515.1"
                     /translation="MKQKGAQQERGLQKNQKGAQEEREKNLKGAQEQRGAQQKPSTEQ
                     QRGAQQQPSVGGRRTTEQTGQAGATGEARGGSVALSEDQRSQIKTVIGRGSGPRLSRS
                     NVNFSISVGTRVPRSVHFVTLPGEIVRIVPQYRGFDYFLVEDEIVIVDPRTLEIVAVI
                     PA"
     gene            complement(3937..>4379)
                     /locus_tag="VM910_02810"
     CDS             complement(3937..>4379)
                     /locus_tag="VM910_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI61516.1"
                     /translation="TKSRVFNPSLMTNGDNGVVFDLKRKDKPRVLLVEDEALVAMMIQ
                     DTLAEFGFQVVGPLGTASEALAAARESHIDAAVLDINLGDGLVYTVAEILGVRGVPFV
                     FVTGYDIDSVDPRFSDIPILQKPIERDMLQKVFMSSIPDRAAVA"
BASE COUNT          957 a         1292 c         1321 g          809 t
ORIGIN      
        1 ccctgatgaa acatcgtgcc atgctcgcgg ccgccaaaca tgccgagctg aatgtcgcga
       61 atggagaccc agccaaaatt gttccagatc acccagacga ccggaatgtt gtattcgacc
      121 gcggtgcaga gcacgtgcgg aaccatggtg aaacagccgt cgcctgtcac cgcgacgaca
      181 gcgcgatccg gagccgcaag ctttgcgcca agcgcaccgg cggcgccgaa tcccatgccg
      241 gagaagcccc aggaattgag catggattgc gggcggcgcg cattccagaa ttgcatgtac
      301 caattgtggc tcactccgac atcgcagcag atgatgccgt ccgtcggaat cacagcctga
      361 caatcggaga cgacgcgttc cggtcgcatc ggcgtcgcgt gtgtcgcaaa attcgggcgc
      421 acgaattcgt cccaagccgc acgccattgc gctatctgag ccagccactg ccgccgcgcc
      481 gcttcatcgc cgttacccga ccgacgttca agctcggtaa ggagctggcg caagaaggtg
      541 cgcgcgtcgg cagcgatggg taaatcaggc ggataattgc ggccgagttc ggcggcatcg
      601 acatcgacgt gaatgagctt ggtgacaggg aaattccagg aatatcccgg cagccacgat
      661 gaggccgacc ggtcgtcgaa ccgcgctccg atcgcgagga caagatcagc gcggcggccg
      721 gcctcgttgg cgggaaaggc accgttgcga ccgataaagc cgagcgacag aggatccgtc
      781 atatcgatgc agcccatgcc gttcggcgac gagatcacgg gtatggcaag ctttttagca
      841 aattccgtga gttcgccgct tgcttccgac aacgccacgc cgtgaccgat gaagatcacc
      901 ggccgttccg cattgaggac catatccagc gcacgcgaca cttcctcagg cgaggcaccg
      961 cttcgctgcg cgttgagcgg cggagtgaca gggggcaagt cgacctcgtc ttcttcctga
     1021 aaaacgttga aagggacatc gacattcacc ggtccgggac ggcccgacag catcgccgtc
     1081 atcgcctgac gcagcgcaag cggcaacatg tcgacgcgtg ttggctggaa gctgcgcttg
     1141 actaccgggc gcacgatgtt gacgaaatcg gcctggtaat gccgattgac ttcctgaaac
     1201 ggactgcgat tgaactgcga ggtcggcaca ttggccgtga tcgccaaaag cgccgatgaa
     1261 tcggtgagcg cgcaggccag cggcataacg atgttgcacg aaccgggacc gcaggaggtg
     1321 agcgtcgcaa ccggctcatg gcgcacgcgg aaataagcgt cggccatgtg cgcggcaacc
     1381 tgctcgtgcc ggggcgatat cagcttgatc cggtcgcgcg cctggtagag cggatcgagc
     1441 ataccgacgt tgccgtgccc gcagatgccg aagacgtaag ggacgccgtt ggcgatcaga
     1501 tattcggtga tcagctcggc gcctttcatc cgtgccatga ataactcccg cagtccgcct
     1561 cagcggactt cttagcatga tgatgcgcgg caagcacaga cgaaacagcc acaattcacc
     1621 cgcgccgctt aatccggcgc acgagataat cgaaattgat ggtgaactgt tcgggctgac
     1681 tgatgtcggc cttgagcttt tcgatgtcag ctcgcgaaaa ctccggcgcc ggatcgcccg
     1741 ccgcttcgac cagcaattgc actatagcgc cgttctccag catgatcgtc ccgatcacag
     1801 cctctgcaat cgtggcgccg gtcaccacca cgccgtggtt tttcaacagc accgcgcggc
     1861 cattgcctag agcgcgcgct actcccgcgc ccattgcgtg cgagcggata agattgatgg
     1921 tgtcggtata gctcccgagc gcctggtaaa acagcgcgcc cggttggcta tagcatttca
     1981 gccctcgtcc ggttgccgaa agcgcaatcg aataggtcgg atgcgtatgg atgatgcaat
     2041 tgacgtcagg ccgcgccttg aaaatctcgg aatggatata gacttcgcta tgccggcgtg
     2101 ccgtcccggc ctcgacggcc ccctccagat cgatggtgag gatattcggc atcgtgatct
     2161 cgtcgagtcc gatgctgtgc gccttcatga aaaataggtt cggatcgtcg ggtaggcgaa
     2221 acgaaatatg accgcgagta aagtcgtcct gaccttcggc tacgagcacc ttccccgcca
     2281 ggataagttg ctctttgagt gatcgatgcg tcgcccgctg tcctgctcct gcccgccgca
     2341 aagattgtcc gctcgcactt ttccccttga ccattgccct tcaccgccct gttgcattgc
     2401 gggtgcaaca gaacagggaa attgccgccg tctcaagagg aaaatgaaga ggcgcttcgc
     2461 acaccctatc ccgtgaccat cactggggct gagtgtccaa ccaatcgtcg acgtctttgc
     2521 gcacgcgatc cttggcccgg ccatagcgtt gttggatctt gccttccagt tgatcgcggc
     2581 ggccattgat ctgggccaga tcgttgtcgg tcaattggcc ccactgctgc ttgattttgc
     2641 ccttcacttc tttccaattt ccttcgactc ggttccagtc catctgccgt ctccaaataa
     2701 tggtttattg ccgaacacag ctaacgtgat cgccacgaat tgggttccgc caagggttcc
     2761 aaaaaacgtc cgaggaactt gccggaacac ggacggccgc aatcgcgttg aatcggtaag
     2821 cacatggcat gaggcgacgc acggcatttc gtgatgcgga aagccgcggc tcagcttttg
     2881 cgggagaaca gaggagagca gcaatgagga acaagttatt ggctacggtc gccgtggcgg
     2941 cggtagtcgg gttcggcggt ttcggcgccg cgcaatcgca gatgagcggc gagtcgaaca
     3001 aggcaacgcc cggcggcgca tcgtcaggcg cgacgcaaca gaagagtggc ggcggtatga
     3061 gcggagccca atcgggtaac aagacgctca acccgtcaag caccgaatcg agcaacccgt
     3121 cgcgaaacca atcggcgcag gaaaagagcg gcaagaccaa cgagcggctc ggccagggtc
     3181 caaaggaaga gcaaaacgaa tcagcccgcg gcgcgcaacg ggaaaacgag gggatgaagc
     3241 aaaagggcgc gcagcaggag cgcggcctgc agaaaaacca gaagggtgcc caagaggagc
     3301 gtgaaaagaa cctgaagggc gctcaggaac agcgcggcgc tcaacagaag cctagcaccg
     3361 aacaacaacg cggcgcgcag caacagccaa gcgttggtgg acgccgcacg actgagcaaa
     3421 ccggccaggc gggagcaacc ggcgaggcac gcggcggctc ggttgcgctg tctgaggacc
     3481 agcgctcgca aattaaaact gtcattggtc gcggaagcgg tccgcgattg agcagaagca
     3541 acgtgaactt cagcatttca gtcggaactc gagttccgcg aagtgtgcac tttgtcacgc
     3601 tgccgggcga aatcgtacgg atcgtgccgc aatatcgcgg cttcgattac ttccttgtcg
     3661 aggacgagat cgtgatcgtt gatccgcgca cgctcgagat cgtcgctgtg atcccggctt
     3721 aacgggactg aatgaaactg cgcagcgggc ccgtgtcgat gttgcgacgc gggcccgctt
     3781 tttgccgacg gtggccccag caacaattct cgcgcttcag cgattatctg tcgcgttgtc
     3841 ttccgcccaa cacggagtga gcctcaaagt tccgattgag tgtggggttg ttgaacgaaa
     3901 ggggcatccc gtccgcaatc tagcgcggat ccgcgttcag gcgacggcgg cgcgatcggg
     3961 tatgcttgac atgaacacct tttgcagcat gtcgcgttcg atcggctttt gcaaaatcgg
     4021 aatatcgcta aatcgcgggt cgacactgtc gatatcgtaa ccggtgacga aaacaaacgg
     4081 cacgccgcgc accccgagaa tttcggccac ggtgtaaacc agcccgtcac cgagattgat
     4141 atcaagtacg gcagcgtcga tgtggctttc acgcgcggcc gcgagagctt cggatgcggt
     4201 gccgagcggg ccgaccacct ggaatccgaa ttcagcgaga gtgtcctgaa tcatcatggc
     4261 gacgagcgct tcatcttcga ccaacaggac gcgcggcttg tccttgcgct tgagatcgaa
     4321 gactacgccg ttgtcgccat tcgtcatcaa cgacggattg aagacgcgcg atttcgtca
//
LOCUS       DATLJT010000129         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2073929,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000129 DATLJT010000000
VERSION     DATLJT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2073929"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VM910_02815"
     CDS             complement(<1..326)
                     /locus_tag="VM910_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61517.1"
                     /translation="MTRVFIVAESARSRERLEDLLDARKIEVVGGASNVEHAAEALAE
                     EDAQVLLVEATNDSVEELLEILAATGLTREAAVVVLLDQSAPVWAGQAVQAGVRGVLS
                     TDADGE"
     gene            complement(323..1180)
                     /locus_tag="VM910_02820"
     CDS             complement(323..1180)
                     /locus_tag="VM910_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVI61518.1"
                     /translation="MGIAGLGEIAERVRRSTVLVEPGSRGSGSGVVWSSDGLIVTNAH
                     VAQGRRPSVTLWDGREFRAEVTERDPRRDLAALHIAADALIAASPADSAKIRPGELAL
                     AVGNPLGFVGALTTGVVHAVAPLRGLGLRPWVQSDVRLAPGNSGGPLADVQGRVMGIN
                     TMVAGRLALAIPSNEVVEFLKGEAQGGWLGVTLQAVRLPRPSQYRFGLVLVDIEPGSP
                     AARASLLPGDILVGSEAGAFSSPADLAESLRGNEPRVLRLEFLRGDYARIRKVSVQVG
                     GRGRGSIAA"
     gene            complement(1183..2094)
                     /locus_tag="VM910_02825"
     CDS             complement(1183..2094)
                     /locus_tag="VM910_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVI61519.1"
                     /translation="MSAEWKAISNELAKATEQAGAHAVAVHGEPRGSSSGVIWRPGVV
                     VTAEHALRRDEEIQVTLPGGTVASAKLAGRDRATDLAVLKCPEATAAASLGDSLSLRV
                     GNVALVVGRTRASGPVAALAFVSLVVRERRLWGGTTLAPYLRLDVALQRTGVGGAVID
                     ANGVIVGIATPRFSPGGAVALPVATVNRVVDALLEKGRIPRGYLGVALQPVRLPENLL
                     KVLQREGKSAAMVLEVETNGPAHKAGVVIGDILVALAGKPVMRLEDVQAHLLEGNIGQ
                     GISAEFLRGGEKRIVSIVVGERPNGGD"
     gene            complement(2329..2859)
                     /locus_tag="VM910_02830"
     CDS             complement(2329..2859)
                     /locus_tag="VM910_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI61520.1"
                     /translation="MLNAIDASPFITVAAVHGLCMGGGLELALSCDLIVADKMARFGF
                     PELRLGLIPGFGGIPRLRREVGNGFIRDLLFTGRTVKAESAQQAGLVAHLAGEGYALQ
                     VARSMAQQVTKFDPKTRTAAKKFIKPIPAGELKKEIELFCKLFNRPVVMESLRRFVET
                     DDPMKHLPVRARHSAS"
BASE COUNT          519 a         1001 c          835 g          517 t
ORIGIN      
        1 tgctctccat cggcgtcggt cgaaaggacg ccgcgaacgc ctgcctgcac cgcttgaccg
       61 gcccagaccg gagccgattg atcgagaaga actacgaccg cggcttccct tgttaggccc
      121 gtagccgcga gaatctcgag caattcttcg acggagtcgt ttgtcgcttc gacgaggagc
      181 acctgggcgt cttcctcggc gagcgcctca gcagcgtgct cgacgttgct cgcgccgccg
      241 acaacttcga tttttcgggc gtccagcagg tcttcgaggc gttcgcgcga cctcgccgat
      301 tccgcaacga tgaagacccg ggtcacgcgg cgatcgatcc tcttccccgg ccgccgacct
      361 gcacgctgac cttgcggatt cgcgcgtagt cgccgcgcag gaattcgagg cgcagtaccc
      421 gcggctcgtt gccccgcaga gactctgcca ggtccgccgg cgaggaaaac gcaccggcct
      481 cactgcccac caagatgtcc ccgggcagca gcgaagctcg cgccgcaggg cttcccggtt
      541 caatgtccac aagcaccaag ccgaagcggt attgactcgg gcgcggcaac cgcaccgctt
      601 gcaaggtcac cccgagccaa ccgccttgcg cctcgccctt tagaaactcc accacttcat
      661 tgctgggaat ggcgagcgcg agtcggcccg cgaccatcgt gttaatcccc atcacgcggc
      721 cctgcacatc agccagcggt ccgccggagt tgcccggagc cagccgaaca tcggattgaa
      781 cccacgggcg tagtcccaag cctcgtaacg gagcaactgc gtgcacgacg ccggttgtaa
      841 gtgcgccgac aaagcccagc ggattgccca ccgcaagcgc cagttctcct gggcgtatct
      901 tcgcggagtc tgctggcgac gcggcgatca gagcatccgc ggcgatgtgc aaagcagcca
      961 ggtcacgtcg tggatcgcgc tcggtgacct cggcgcgaaa ctcgcggcca tcccacaggg
     1021 tcacactggg ccgcctgcct tgcgccacgt gcgcattggt gacgatgagc ccgtcgctac
     1081 tccagacaac tcccgagccg ctgccgcgac tgcctggctc gaccagcacc gtcgatcgcc
     1141 tgacgcgttc cgcgatttcc cccaagcccg cgatgcccat tttcaatcgc ctccattcgg
     1201 ccgctcgccc acaacgatgc tcacaatgcg cttctcgccg ccacgcagaa actccgcgct
     1261 gatcccctgg ccgatgttcc cttcaagcag atgcgcctga acgtcctcca ggcgcatgac
     1321 cggtttgccg gcgagtgcca cgagaatgtc accaatcacc acgcccgcct tgtgcgccgg
     1381 gccgttggtc tccacttcga gcaccatcgc agcggacttt ccttcgcgct gtaagacctt
     1441 cagcagattt tccggcagcc gcaccggctg cagcgccaca cccagatagc cccgtggaat
     1501 gcgtcctttt tcaagcaacg cgtcaacgac gcggttaacc gtcgccactg gcagggctac
     1561 ggccccgccc ggcgaaaatc ggggcgtcgc gatgcccacg atgacgccgt tcgcgtcgat
     1621 cacggctccg ccgactcccg tgcgctgcag tgccacatcg agtcgcaaat aaggagcgag
     1681 cgtggttccg ccccaaagcc gtcgctcccg caccacaagg cttacgaacg ccagcgccgc
     1741 aacgggcccg ctcgcgcgcg tgcggccgac gaccaacgcc acgttcccga ctcttagcga
     1801 aaggctgtcc ccgagcgacg ccgccgctgt tgcctcagga cacttcagca cggcgagatc
     1861 ggtcgcacgg tcacgccctg ccaatttagc gctcgcgacc gtgccgccgg gcaaggtcac
     1921 ctgaatttcc tcgtcgcgac gcaaagcgtg ttccgcagtc acaaccacgc cgggccgcca
     1981 gattacgccg ctggatgagc ctcgtggctc gccgtgcacg gccacggcgt gagctcccgc
     2041 ttgttcggtg gctttcgcca attcattcga aatcgccttc cattctgctg acatggttcg
     2101 tgcctccgag ccaagaatgc ccgagtcaca cgcctccgca catccgccat tcggctaggt
     2161 gcctggtttc cgcggcaacg agaaaggtcg gctgcttggt gtagttctta gcggttggcg
     2221 ggcgagttga cctcgcatta ggcgtaaact cttcgaggcc gtcgatctca agcctgactt
     2281 cgtttcggcc tgggcgtcaa tgttgacgcc aaagcgcctt gggaatgatc aggatgcgga
     2341 gtggcgtgct cgaaccggca gatgcttcat ggggtcgtcg gtctccacaa agcggcgcaa
     2401 gctttccatc acgaccggtc ggttgaacaa tttgcagaaa agctcgatct ccttcttgag
     2461 ttccccggcg ggaatcggct tgataaattt cttggcggcc gtgcgcgtct tggggtcaaa
     2521 ctttgttacc tgctgcgcca tggagcgcgc cacctgtaag gcatagcctt cgccagccag
     2581 atgggccacg agtccggctt gttgagcgct ttcggcttta acggttctgc cggtaaagag
     2641 caagtcgcgg atgaatccgt tgccgacctc gcgccgcaag cgcggaatgc ccccgaagcc
     2701 agggatcaag ccaagccgca gctccggaaa gccgaagcgg gccatcttgt cggcaacgat
     2761 cagatcgcag ctcagcgcca gttcgagccc gccgcccatg cacaagccat gcaccgcggc
     2821 gacggtaata aagggcgacg cgtcgatcgc attcagcact tggtggattc gc
//
LOCUS       DATLJT010000130         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3681845,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000130 DATLJT010000000
VERSION     DATLJT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3681845"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1067)
                     /locus_tag="VM910_02835"
     CDS             complement(171..1067)
                     /locus_tag="VM910_02835"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI61521.1"
                     /translation="MTLSNETTLAPAESAAGFSVVPTDAALGAEVRGVDLRNLDATSF
                     ERVMHAWYDHSVLLFRGQSLSDQELIAFSRRLGALDWAPIQENGRRFVEGLPEIYIVS
                     NVRVKGEPIGSLGDGEAVWHTDMSYLDVPPKASLLYALEVPTAGGNTSFCTMYRIYEG
                     LPARLKERIAGLKIKHDGTYNSGGYVRQGITPTDDPRTSPGAVHPLVCTHPATGRRML
                     YLGRRRNAYLIGLELAESDVLLNELWSVVDRPEFAWEHVWRVGDLVMWDNRCTMHRRD
                     AFDPASRRIMHRTQVKGDERPS"
     gene            complement(1376..2206)
                     /locus_tag="VM910_02840"
     CDS             complement(1376..2206)
                     /locus_tag="VM910_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61522.1"
                     /translation="MTELNRAGNARPVDAVARAPRLPLGGIKRDHLVYVASIIGLIML
                     WHLIATTFFKPQFFPPPLLVLSTGREMLESGELMEHINISLQRILAGFLIGSAIAAPA
                     GLLMGSIPIVRAIFDPYVQFFRFVPSLAWLTPVVIWFGIGETSKVLIIVYTTIFIVLI
                     NTMVGVSHIAPNKLRAAACLGATPLKAFIFVTLPATLPFILTGMRLAMGNSFATVVAA
                     EMIAADSGLGYLIFNSRLWMATDKIFIGIVCLGALGLITDRFFRYLIVRFAHQYGPIE
                     "
     gene            complement(2203..>2715)
                     /locus_tag="VM910_02845"
     CDS             complement(2203..>2715)
                     /locus_tag="VM910_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61523.1"
                     /translation="DALFNWLRAIDNVAFGLRMQGMSKERRHAVARDYLHLVHLGGQE
                     NKYPGELSGGMKQRVQIARVLASNPKVLLMDEPFGALDAQTRGRMQSELVEIWHKTSK
                     TIVFITHDITEAVLLADRIGVMTKGPESRLDAIIPIDLPRPRRRSSRGFGEMWERINA
                     LIENEEANGP"
BASE COUNT          540 a          843 c          834 g          498 t
ORIGIN      
        1 aatcataatc cgtaggtcgc cggttcgatc ccggcccccg ctaccacaca gaaatcaatg
       61 aaattgttca cgctcaacgc gacgccgagg tcagggcgag gaggccaagt agatgacgtc
      121 gcctgatcat gacacccggc cggggtgccg ctctgattat ttgcgcctga tcaggagggt
      181 cgctcatcgc ccttgacttg agtgcggtgc atgattcgcc ggcttgccgg gtcgaatgca
      241 tcgcgtcgat gcatggtgca gcgattgtcc cacatgacga gatcgccgac cctccagacg
      301 tgttcccagg cgaactccgg ccgatcaacg acgctccaga gctcattgag caacacgtcg
      361 gactcggcga gctcaagtcc gatcagatag gcgtttcggc ggcggccgag ataaagcatg
      421 cgacgtccgg tcgccggatg cgtgcacacc aacggatgca ccgctcccgg cgacgtgcgc
      481 ggatcgtcgg tcggcgtaat accttgccgc acataaccac cgctattgta ggtgccgtca
      541 tgcttgatct tgagcccggc aattcgctcc ttcagccggg caggcagccc ctcatagatg
      601 cgatacatag tgcaaaacga ggtattgccg ccggcagtag gcacttcgag cgcatagagc
      661 aggctcgcct ttggcggcac atcaaggtac gacatgtcgg tgtgccatac cgcctcgccg
      721 tcgcccaagc tgccgatagg ttcccccttc accctgacgt tggaaacgat atagatctcc
      781 ggcagacctt cgacgaagcg tcggccgttt tcctggattg gtgcccagtc gagggctcct
      841 aagcggcggc tgaatgcgat caactcctgg tcgctcagcg attgaccgcg aaacagcagc
      901 accgaatggt cgtaccacgc gtgcatgacg cgctcgaacg atgttgcgtc aaggttgcgc
      961 agatcgacac ccctgacttc ggcgccgagc gccgcgtcgg tcgggaccac cgaaaacccg
     1021 gccgcactct cagctggcgc caaggttgtt tcattgctca atgtcatggc tcgctcccct
     1081 cccagaagac ttaccgaggg tccaactcag catgcttcct agccagcttg ctggcaagct
     1141 ccctcaaggc ctgacctgtc gcgcggcgcc agcgtcgacg atatcgtcac cacctgcgca
     1201 gtggcgctga caatggcgta aggcttgggg gcgcatcacc ccccgtggcc agcctgggcg
     1261 accgcgagcc cctgctggcc tgggcgttca gtcgaacagc gagctcgaag cagaaaatgc
     1321 cgtgttccgg catcgtcggc ccgcgtgagc ctgtcaatcc tcaagatccg gaaggctatt
     1381 cgatcggccc atattgatgg gcgaagcgca cgatgagata gcggaagaag cggtccgtga
     1441 tgaggccaag cgccccgaga catacgatgc cgatgaagat cttgtcggtt gccatccaga
     1501 ggcgcgagtt gaatatcaga tagccgaggc cggaatctgc cgcgatcatc tcggcggcga
     1561 ccacggttgc gaacgaattg cccatggcaa ggcgcatgcc ggtcaggatg aacggcagcg
     1621 tcgccggcag cgtcacaaaa atgaatgcct tgaggggggt ggcgccgagg caggccgccg
     1681 cgcgcagctt gttgggggcg atatgcgaga ccccgaccat ggtgttgatc agcacgatga
     1741 agatcgtcgt atagacgatg atgagaactt tcgatgtctc cccgatgccg aaccagatca
     1801 ccaccggggt cagccatgcc agcgagggca cgaaacgaaa gaactgcaca taggggtcga
     1861 agatcgcgcg gacgatggga attgagccca tcagcaaacc ggccggagca gcgatcgcgc
     1921 tgccgatcag gaatcccgcg aggatgcgtt gcaggctgat gttgatgtgc tccatcagtt
     1981 cgccgctttc cagcatctcc cgtccggtcg acagcaccag gagcggcggc ggaaagaact
     2041 gcggcttgaa gaacgtcgtg gcgatcaagt gccacagcat gatcaacccg atgatggagg
     2101 cgacataaac gaggtgatcg cgcttgatcc cgccgagcgg caggcgcggc gcccgcgcca
     2161 cagcgtcaac gggcctggcg tttcctgccc ggttgagctc cgtcatggtc cgttcgcctc
     2221 ttcgttctcg atcagcgcgt tgatgcgctc ccacatctcg ccgaagccgc gactgctgcg
     2281 ccggcgtggc cgcggcaggt cgattgggat gatcgcatcg agccgcgatt ccggcccctt
     2341 ggtcatgacg ccgatgcgat ccgccagcag gactgcctcg gtgatatcgt gggtaatgaa
     2401 cacgatcgtt ttgctggtct tgtgccagat ctccacaagc tcgctctgca tacgtcctcg
     2461 ggtctgagca tcgagcgcgc cgaaaggctc gtccatcagc agcaccttgg ggttgctcgc
     2521 aagcacgcgg gcgatctgca cccgttgctt catgccgccg gacagttcgc cgggatactt
     2581 gttctcctgg ccgccgaggt gcacgagatg aaggtaatcg cgggcaacgg cgtgccggcg
     2641 ctccttggac atgccctgca tccgcaggcc aaacgccacg ttgtcgatgg cgcgaagcca
     2701 gttgaacagc gcgtc
//
LOCUS       DATLJT010000131         7268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1155130,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000131 DATLJT010000000
VERSION     DATLJT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155130"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VM910_02850"
     CDS             <1..285
                     /locus_tag="VM910_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroflavonol 4-reductase"
                     /protein_id="HVI61524.1"
                     /translation="LMAAVSGRRKYFVPVPGQLAELAASMIEFKANYLTRRTPSATVE
                     GVRIARRATALSIDKARHELGYEPRPIEPMLKETIARMMGVPASKVLKHA"
     gene            278..904
                     /locus_tag="VM910_02855"
     CDS             278..904
                     /locus_tag="VM910_02855"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI61525.1"
                     /translation="MPDSLGQITGALLGGWVTAWPTRVLSLIWLAWVVSWVVAAFWSG
                     RTKAHVRTRDSWVYRLPILLGAIMLLPLTGHLLGAKPLYHVGDSGTYVLAVVVLLGIC
                     FTWWARIHLGQFWSNAITHKEDHRIVDTGPYGLVRHPIYTGLIIALLASGIAIATWPA
                     ILGVLLVSFGEWQKARMEERFLTAELGAEAYAAYCRRVPMIVPFLRGS"
     gene            complement(934..1884)
                     /locus_tag="VM910_02860"
     CDS             complement(934..1884)
                     /locus_tag="VM910_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVI61526.1"
                     /translation="MVLAFFACHGGGSAIAGQIGAVKHAAAEVTGDRTAVQGNSSPGA
                     PPLAATAPLGEPALAQAHAYLFRGALGLIFSRGMDKLAAKIQRAGIITNVYEFTICHL
                     ITATAIEDYKSKPFPIVLIGHSMGGRCALEFSEKLKDEGIPVSLVVTIDPAHLSPDVP
                     NNVDRFINIFLAKDVLGGGDIKPAQDFRGHYASYDLSRRDEVLHINIDKMDAVHQQLA
                     AKVVEAVGSPAKTEGETVPLRYTVPPKTVIELWDSGMWVTARPGDTLQTIAAQYRLPT
                     WALTQSNQVAENASLVPGQRVVVPRHLAPPAAAEPISSKR"
     gene            complement(2065..2814)
                     /locus_tag="VM910_02865"
     CDS             complement(2065..2814)
                     /locus_tag="VM910_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61527.1"
                     /translation="MNGIGSRTVRFAGFSIRAAQWLGALKALAIVVVLIGLPLSASAA
                     PSAAPAPAHVYLMRGVLNIFSLGMDTIAARLEQQGISASVHNHLAWAAVADEAAALYR
                     SGRVKTIILVGHSSGATCLPDIVARLDQQGVPVKLAIGLDSVFHTNLTGRVGRYINFY
                     VASGPGTRVEKTTGFRGTLENVDVERVPGVGHLTIDKNELIQQKVIAAIDAAVVSGPS
                     EPAPTARPEAAATAAPATAAAKPAGPRRAAN"
     gene            3087..3866
                     /locus_tag="VM910_02870"
     CDS             3087..3866
                     /locus_tag="VM910_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI61528.1"
                     /translation="MTDNSVILEKRGQAFWITINRPDKRNALNGDVIAGIARGYREAH
                     ADKEVRVIVLTGTGDKAFCAGADLQNSGAAFARDFSVPNVDYADLLRQSQNATKPAIA
                     RVGGVCMAGGMGLLCMTDMAIAAETAIFGLPEVKVGVFPMQVLSLLQSIAPPRQVAEW
                     ALTGEPFDAKTALAGGLLNYVVPAAELDARVEWLIGRIVDKSPTAIRRGKYAMRAIAS
                     MSFDESIAYTESQIALLAMTEDAKEGLKAFGEKRKPVWTGR"
     gene            complement(4610..4741)
                     /locus_tag="VM910_02875"
     CDS             complement(4610..4741)
                     /locus_tag="VM910_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61529.1"
                     /translation="MNLRQITATVFAAIMIGVAFWATMGPPPKEEQISMIGMHRLAP"
     gene            complement(5066..6046)
                     /locus_tag="VM910_02880"
     CDS             complement(5066..6046)
                     /locus_tag="VM910_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HVI61530.1"
                     /translation="MRQVVEPFRRDAVEEGDPAADSKAFRRCLGQFSTGVGVMTTIAN
                     GEPVGVTANSFSSLSMDPPLVLWSIAHTSRSSAAFKNAKHFAVNILAVEQVEYSQRFA
                     RPAENKFEGVDWRAGVLGSPILPNVLASLECVLETTLQGGDHVILIGRVKKYSRYAGN
                     ALLYAQGRYAVAEDHPSLQLKPSGAVKADAELNVSEMRLMALLAYVQMYASEAFDKYR
                     QSQGLNIAQGRILFVLSGENQLSLQEIVARSFLPRDSAEDALDSLTERGYVGGRLKAF
                     ALTESGRNLFTRLIAQVDKFEAEQLSGISQQDLAVTRSVLEKLYGRLRSS"
     gene            complement(6796..>7268)
                     /locus_tag="VM910_02885"
     CDS             complement(6796..>7268)
                     /locus_tag="VM910_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61531.1"
                     /translation="DEAVLRNMGDEALGIVTACWYSAELDNPLNKQFAPAFRKEFSYD
                     PGFYAASTYVEAAVLESALKAVAGKVEDKDAFMAALRATNVDTVRGPVRFDEFGNVIG
                     NVYIRKVARKDGRLVNSVIKTYPDVSQFWTYDPKQFLANPVYSRDFPPAKNLEK"
BASE COUNT         1386 a         2204 c         2233 g         1445 t
ORIGIN      
        1 ctgatggcgg ccgtcagcgg ccgtcgcaag tattttgttc cggttcccgg ccaactcgcc
       61 gaactggccg cgtcgatgat agagttcaag gccaactacc tgacgcgccg cacgccttcg
      121 gccaccgtcg aaggcgtccg catcgcgcgg cgggcgacgg cgctctcgat cgacaaggcc
      181 cgccacgagc tcggctatga gccgcggccg atcgagccga tgctgaaaga gacgatcgcc
      241 cgcatgatgg gcgtgcccgc aagcaaggtt ttgaagcatg cctgacagtc tcggccaaat
      301 caccggcgcc cttctgggcg gctgggtcac ggcctggccg accagagtgc tgtcgctgat
      361 ctggcttgcc tgggtggtga gctgggtcgt tgccgccttc tggtccggcc gcaccaaggc
      421 gcatgtgcgt acgcgcgatt cctgggtcta tcggctgccc attctgttgg gcgcgatcat
      481 gctgctgccc ctgaccgggc atctgctggg cgccaagccg ctctatcacg taggcgatag
      541 cggaacatat gtgctggcgg tcgtggtgct gctcggcatc tgcttcacct ggtgggcgcg
      601 gatccatctc gggcaattct ggtccaacgc gatcacgcac aaggaagacc accggatcgt
      661 cgataccggg ccttacggcc tggtgcggca tccgatctac accggcctga ttatcgcgct
      721 cctggcctcc ggcattgcga tcgcgacatg gcccgcgatc cttggcgtac tgctggtctc
      781 gttcggcgaa tggcagaagg cgcggatgga ggaacgcttc ctcaccgccg agctcggcgc
      841 cgaggcctat gcggcctatt gccgccgtgt gccgatgatc gtgccgtttc tccgcggttc
      901 ctgacgcaat cctcgtcccc caggcgcgcc gcttcagcgc ttcgacgaaa tcggctcggc
      961 ggccgcaggc ggcgccagat ggcgcggcac gacgacgcgc tgaccgggca cgagcgatgc
     1021 attttccgcc acctggttcg attgcgtcag cgcccacgtc ggaagccgat attgcgcggc
     1081 gatcgtctgc agcgtgtcgc ccggacgcgc cgtcacccac atgccgctgt cccacagctc
     1141 gatgacggtc ttcggcggga cggtatagcg cagcgggacg gtttcgcctt cggtttttgc
     1201 cggcgagccg actgcctcga cgaccttggc ggctagctgc tggtggacgg catccatctt
     1261 gtcgatgttg atgtgcagga cctcgtcgcg gcgcgaaagg tcatagcttg cataatggcc
     1321 gcgaaaatcc tgggccggct tgatgtcgcc gccgccgagc acgtccttcg ccaggaagat
     1381 attgatgaag cggtcgacgt tgttcggcac atcggggctg agatgggccg gatcgatggt
     1441 taccacgagg ctgaccggga tcccttcgtc cttcagcttt tccgagaact cgagcgcaca
     1501 gcgtccgccc atcgagtggc cgatcaggac gatggggaag ggtttgctct tgtagtcctc
     1561 gatcgccgtg gcggtgatga ggtggcagat ggtgaactcg taaacattgg tgatgatgcc
     1621 ggcgcgctgg atcttggccg caagcttgtc catgccgcgc gagaagatca ggccgagcgc
     1681 cccgcgaaac aggtaggcgt gcgcttgcgc cagggccggt tcgcccagtg gcgcggtggc
     1741 cgccaaaggc ggcgcgccgg gcgaactatt gccctggacg gccgtccgat cgcccgtgac
     1801 ctcggcggcg gcatgcttga ccgccccgat ctgtcccgcg atggcagagc ccccgccgtg
     1861 acaagcaaaa aaggcaagaa ccactgccgc gagaacgctc gtcgcccggc tttttataga
     1921 tcggttcctg ctcacgtacc cgcacattcg tcgctattgg cgccgacaac gccgatgcgc
     1981 gccgtccgtc gggttcccag atggcggacg aatgggcgga atttgaggca ctgccgaacg
     2041 ccgcaagcgg cattcggctc ccggtcagtt ggccgcgcgc ctcgggcccg caggcttcgc
     2101 cgccgcagtg gcgggagccg cggttgcggc cgcctccggc ctcgcggtcg gagcgggctc
     2161 gcttgggccg ctgacaacgg cagcatcgat ggcggcgatg accttctgct ggatcaactc
     2221 gttcttgtcg atcgtgaggt gacctacgcc gggcaccctt tcgacatcga cattttcgag
     2281 cgtgccgcgg aagcccgtgg tcttctccac ccgcgtgcct ggtccgctgg cgacatagaa
     2341 gttgatatag cgtccgacac ggccagtcag gttggtgtgg aacacggagt cgagcccgat
     2401 cgccagcttg accggaaccc cctgctgatc cagccgcgcc acgatgtcgg gcaggcaggt
     2461 cgcaccggac gaatggccga ccaggatgat ggtcttgacg cgcccgctcc tgtaaagcgc
     2521 ggccgcttcg tcggcgaccg cggcccaggc gagatgattg tggacggaag cgctgatgcc
     2581 ctgctgctcc aggcgcgcag cgatcgtgtc catgccgagg gagaagatgt taagcacccc
     2641 gcgcatcagg tagacgtgcg caggtgccgg cgctgccgaa ggcgcggcgc tcgcagacaa
     2701 gggcaggccg atcagaacca ccacgatcgc caatgccttc agcgcgccca accattgtgc
     2761 agcgcggatc gaaaacccgg cgaaacgcac agttctgctt ccaattccgt tcatgattcc
     2821 ctcggtatgc cccacagtta tcgaggtata gcgtcggttt tcccatctgc aatgaaagac
     2881 gcccaccaaa atgccttttt ccggacatcc gcggcggaat tgcaacagat aggccgcgcc
     2941 cgatacgctc gataagcttc tgattggcat gccgttattt gcgtcttgtg cggagccgtc
     3001 gcaaagcccg gcggaaacgc ccttgcggcg gcaacaattc cggcggcaaa tgccgccttc
     3061 gacggcaacg ggcggatgga actgcgatga ccgacaacag cgttatcctc gaaaagcgcg
     3121 ggcaggcgtt ctggatcacc atcaaccggc ccgacaagcg caacgcgctc aacggcgacg
     3181 tcatcgccgg catcgcgcgc ggctaccgcg aggcgcatgc ggacaaggag gtccgggtca
     3241 tcgtgctgac cggcaccggc gacaaggcgt tttgcgcggg ggcagacctg cagaactcgg
     3301 gcgcggcgtt tgcgcgcgat ttctcggttc cgaatgtcga ctatgccgac ctgttgcggc
     3361 agtcgcagaa cgccacgaaa cccgccattg cccgcgtagg cggcgtctgc atggcaggcg
     3421 gcatgggact tctgtgcatg accgacatgg caatcgcggc cgagacggcg attttcgggc
     3481 tgcccgaagt gaaggtcggg gtgtttccga tgcaggtgct cagtctcttg caatcgatcg
     3541 cgccgccgag gcaggtcgcc gaatgggcgc tgaccggcga gcccttcgac gcgaagaccg
     3601 cgcttgccgg cggactgctc aattatgtcg tgcccgcagc cgagctcgac gccagggtcg
     3661 agtggctgat cggccgcatc gtcgacaagt cgccgactgc gatccggcgc ggcaaatacg
     3721 cgatgcgcgc gatcgcctcg atgtcgttcg acgagagcat cgcctacacc gagagtcaga
     3781 tcgcgctcct ggcgatgacc gaggacgcaa aggaagggct caaggcgttt ggcgagaagc
     3841 gcaagccggt ctggaccgga agatgaggcc gccctcattc tcgccgtgag gggtaaggca
     3901 gcaatatttc gattcaaagt ttcaaacagc cgttttcgca tcgggatcga tgacacgact
     3961 ccgcattccc gcggctcgat ggcgcccgag ctttgcaaga accacgtccc tcgataacgg
     4021 agagggcgca gggaatgccg ggtggccgac gcacccgcag cctcgcgtgc gatgaagagt
     4081 agcacacgag taagtcacca caggttcgcc gaaacgatcc ggcactccct gcgcgatggt
     4141 ttggcggctt attccgcgct cttcccggag accagacttt gttgtctccg tcgtctgcgg
     4201 atgactccgc agacttgacg tcagccttgg gacgtcagaa cgacacggct tcgccgacgc
     4261 aaggacaatc gtacgtcttg cgacgccact cgcgtccatc gcatcccgct tcctacgttc
     4321 gtgacgatcg cgaagcgccc ctttttttga aagcgggacg gcgcaaatgt gctgttgatt
     4381 tgggtctttc gacaatgtcg cggcgctgcg gcagattggc acgacgggca atttgcgcat
     4441 gacacgcatg cacggttttc gctttgcttg tcgggcaaat cagtttgcct cacacgcatc
     4501 tgcctcgcat cggcttgatg gcggcacatt cggcgaagtt cattgaaagt tactcgacgg
     4561 attttgccga ataattggaa gtggccggca gcggccgccc aatttcaggt caaggcgcga
     4621 gccggtgcat gccgatcatg ctgatctgct cttcctttgg aggagggccc attgtggccc
     4681 agaacgccac gccaatcatg atggccgcga aaacggttgc ggtaatttgt ctgagattca
     4741 ttggtttcaa ttcgttcggt tcgaaatgct tccgatttat cggtgagtaa cgacgccggt
     4801 gtgacaagca atttgcgtgc cgctgcgcat gtcgggaacg acactgttct gagcactagg
     4861 gcgtggactt actaaaagca acgagcccct tcgtggctca cgtctgcttt gaccgaatgg
     4921 gactaaaaat ttaacatttt gggacatgag gcgtcggacg gttcctcatc cgcggtgctg
     4981 tcctcagtgg atatgtggac gtcgcaaaat cgcctcgacg agtccttcga ctgtgcgacc
     5041 tatcattccc tggatgtaaa ccgcgctatg aagacctcaa gcggccgtag agtttctcga
     5101 gcacgctacg cgtgactgca agatcctgct gtgagatgcc gctgagctgc tcggcttcaa
     5161 atttgtccac ctgggcgata agtcttgtga aaaggttgcg cccgctttct gtgagagcaa
     5221 atgctttcag ccggccccca acatagcccc tttcagtgag actatcgagc gcatcttccg
     5281 cggagtcgcg aggcaaaaaa gatcgcgcga cgatctcctg cagcgacaat tggttttctc
     5341 cgctcagcac aaacagaatg cggccttgcg cgatgttgag cccttgcgac tggcggtact
     5401 tgtcgaaagc ttcagacgcg tacatttgga cgtaggccaa caaggccatg agacgcattt
     5461 cgctgacgtt caactcagcg tcggctttca cggcacctga aggcttaagc tgcagggagg
     5521 gatgatcctc cgctacggcg taccgccctt gcgcatacag gagcgcattg cctgcgtagc
     5581 gcgaatattt ctttacacgg ccaatcagaa tgacgtggtc cccgccctga agcgttgtct
     5641 caagcacgca ctccagcgag gctaatacgt tcggcagaat tggagaaccc aggacaccgg
     5701 cgcgccaatc gacgccttcg aacttgttct ctgcggggcg cgcgaatcgc tgtgagtact
     5761 ccacctgttc aacagcgagg atattaacag cgaagtgctt cgcattcttg aaagctgccg
     5821 aactgcgtga agtgtgcgca atggaccaca gcaccagggg cggatccatc gatagcgacg
     5881 aaaaagaatt cgcggttaca ccgactggtt cgccgttcgc gatggtagtc ataacaccga
     5941 cgcccgttga gaattgtccg agacagcgcc taaaagcctt tgaatcggcc gctggatctc
     6001 cttcttcgac agcatctctg cgaaagggct ctacgacttg ccgcatttcg ctcccctggg
     6061 gtgaggcccg acgaggctca atcttatgca aatcctgggt tttgcgctat gggcaggaag
     6121 tccgctccgg ggtcaacagc cgtaagccga gcaaatgttt tccagtttgt gcctcaaagc
     6181 agacgtaagg cgtttatgag tacacgccct aaatctatct gacgcctatc tgaagcaaaa
     6241 aagcccgcca ttcgcgacgg gctcaagtca gggaggaaag ccgtacgcag tacggcgacg
     6301 gcaaaggtaa gtgattcttg aactgtgctg tgtgttctag ttcacacact gagacgaccg
     6361 aattcatgca ccgcctgcga actctctcga tcaaacctgt gaatgtgctg gctgggccac
     6421 acctaaatcc tggcgcctgg ggtatgaggg cagccgtcac ggccgcaaga aagacgggcg
     6481 actgggggct gacctgacca gctcagaacc tgagagccgc tccagaggga attggggcgg
     6541 ctcttttttc tttgaaggtt ctaaagcgcg atgagattag gttgatattg ctgcgccgga
     6601 ggtgcgctcc ctcccctgtt tgcgacctta gagggcaggg ccctttccgc atcgtcatgg
     6661 ccgcgcttgt cccggccatc cacgtcgtgg caaatcgaaa gaaaggcgtg gatgcccggg
     6721 tcgagcccgg gcatgacgag tccgtgggac cactaaaccg aaaatcaacc tcgcgaaaat
     6781 ccacgacgta atccgttact tctcgagatt tttcgccggc ggaaaatccc gcgagtacac
     6841 gggattggcg aggaattgtt tcggatcgta ggtccagaac tggctgacgt cgggataggt
     6901 cttgatcacc gaattgacca ggcgtccatc cttgcgcgcg accttccgga tgtagacgtt
     6961 gccgatgacg ttgccgaact catcgaacct gaccggcccg cgcaccgtgt ccacgttggt
     7021 tgcgcgcaac gccgccatga acgcatcctt gtcttcgacc ttgccagcga ctgccttcaa
     7081 tgctgattcc agtactgccg cctcgacata tgtgctggcg gcgtagaagc ccgggtcgta
     7141 tgaaaattcc ttgcggaagg ccggagcaaa ctgcttgttg agcggattgt cgagctcagc
     7201 ggaataccag cacgccgtta cgatcccgag cgcctcatcg cccatgttgc ggagcaccgc
     7261 ttcgtcca
//
LOCUS       DATLJT010000132         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2992964,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000132 DATLJT010000000
VERSION     DATLJT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992964"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM910_02890"
     CDS             <1..402
                     /locus_tag="VM910_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI61532.1"
                     /translation="VLYIYTGWGDNWKDPDTEKFYYTKGPGLAYDAARYIAEKKVVLL
                     ALDNPFTDPVAEGFLQGKAPPPAGAPEGLPFAVHHTNLAESGIYQIQNANLGALARDK
                     VWLSCTIILPLRAQGNAGSPVRPVAIGAPVQ"
     gene            complement(466..2226)
                     /locus_tag="VM910_02895"
     CDS             complement(466..2226)
                     /locus_tag="VM910_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI61533.1"
                     /translation="MLARADTVATIAESWLARFEAALAAPGRPRLERLFHADSHWRDL
                     LALTWHLKTVSGSDAIVRELATHAGRARPAGFKIDPHRTAPRNVRRAGTDAIEAIFGF
                     ETAEGRGSGVLRLTPDADDGGTVKAWTLLTALDEIKGHEERLGRSRPQGKAYSRDFRG
                     PNWLDLRKAAAEYGDRDSTVLVVGGGQAGLSIAARLAQLGVDTLIVDREARIGDNWRK
                     RYHALVLHNQVHVNHLPYMPFPPNWPTYIPKDKLAAWFEAYVESMELNYWTATEFEGG
                     SYDEKAKRWSVVLRRADGTKRQMHPRHVVMATGVSGIPSLPDIPSLRNFGGKVLHSSQ
                     YDDGEAWRGKSALIIGSGNSGHDIAQDLHSAGAKVTLVQRSSTMIVNVEPSAQLQYAL
                     YDEGPSLEDCDLITTSMPLSLVRKSHIALTETARNMDKELLDGLERRGFKLDFGEDGT
                     GWQFKYLTRGGGYYFNVGCSDLIVKGEIGLAQFSDVASFVPTGARMRSGETLAADLIV
                     LATGYQGQEHLVSKLFGDDVAGRVGPIWGFGDGQELRNMFARTAQPGLWFIAGSFAQC
                     RIYSKYLGLQIKACELGLLP"
     gene            2345..>2773
                     /locus_tag="VM910_02900"
     CDS             2345..>2773
                     /locus_tag="VM910_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61534.1"
                     /translation="MRTKRMFLTAIVAVGIGLAGASLASAQSYPDRLIKIVVPYPPGG
                     PADVAARLVTQPLSTRLGQSVIVENQPGGGGRTGAKFVAHASADGYTLLLGGTNPNAI
                     ASLYRTLDFEPIKDFAGVGLIGFDSNALVVNPAVPAKTLQE"
BASE COUNT          491 a          888 c          876 g          518 t
ORIGIN      
        1 gtgctctaca tctacaccgg ctggggcgat aattggaaag accccgatac ggagaaattc
       61 tactacacca agggtcccgg tctcgcctat gacgcagctc ggtacatcgc ggagaaaaag
      121 gtcgtgctgc tcgcgctcga caatccgttc accgatcccg tcgccgaggg gttcctgcaa
      181 ggcaaagcgc cgccgcccgc gggtgcgccg gaaggcctgc cgtttgcggt ccatcacacg
      241 aacctcgcgg agtcaggcat ctatcagatc cagaacgcca atctcggagc actcgcgcgg
      301 gacaaggtgt ggctgtcatg caccatcatc ctgccgctgc gagcgcaggg aaatgccggg
      361 tcgccggtgc ggccggttgc gattggcgct cccgtgcagt agcgcgcggt ctcttaggca
      421 tgcgaggagc gtcgcggacg cccgacgtca cggtggtata cgcactcagg gcagcaggcc
      481 cagctcgcag gccttgatct gtaggccgag atatttcgag tagatgcggc attgggcgaa
      541 gctgccggcg atgaaccaaa ggcccggttg cgcggttcgc gcgaacatgt tgcgcagttc
      601 ttgtccatcg ccgaatcccc aaatcggccc gacgcggccg gcaacatcat cgccgaacaa
      661 tttgctgacc agatgctcct gcccctggta gcctgttgcc agcacgatca ggtcggcggc
      721 aagtgtttcc ccgctacgca tacgcgctcc tgtcggcacg aagctggcga catcggagaa
      781 ttgcgccaat ccgatctcgc ctttgacgat cagatcggaa cagccgacgt tgaagtagta
      841 accgccgccg cgggtgaggt acttgaactg ccaaccggtt ccgtcttcgc cgaaatcgag
      901 cttgaagccg cggcgctcga gcccatcgag caattctttg tccatattgc gggcggtttc
      961 ggtcaatgcg atgtggcttt tccgtactag tgaaagcggc atcgacgtcg tgatgagatc
     1021 gcaatcctcc agcgaaggcc cttcgtcata gagcgcgtac tgcagttgcg cgctcggctc
     1081 gacattgacg atcatggtcg agctgcgctg cacgagcgtg accttcgccc cggccgaatg
     1141 cagatcctgt gcgatatcat ggccgctgtt gcccgagccg atgatgagag cgctcttgcc
     1201 cctccacgcc tcgccatcgt cgtactgact ggagtgcaga actttaccgc cgaagttgcg
     1261 caagctcgga atatcgggaa ggctcggaat gccgctcacc ccggtggcca tcacgacgtg
     1321 gcgcgggtgc atctgccgct tcgtgccgtc ggcccgacgc agcacaacgg accatcgttt
     1381 tgctttttcg tcgtagctgc cgccttcgaa ttcggtggcg gtccaatagt tcaactccat
     1441 gctctccaca taagcctcga accaggcggc gagcttgtcc ttgggaatgt aggtcggcca
     1501 gtttggcgga aagggcatgt acggcaggtg gttgacgtgc acctggttgt gcaggaccag
     1561 cgcgtgatag cgcttgcgcc agttgtcgcc gatgcgcgct tcacggtcga cgatcagcgt
     1621 atcgacgccc aattgcgcga ggcgcgcggc gatcgaaagc ccggcctgtc ccccgccgac
     1681 aacgagcacg gtggagtcgc ggtcgccata ttcggcggcg gccttgcgca ggtcgagcca
     1741 attaggtccg cggaaatcgc gcgaataggc tttgccttgc ggccgcgatc gtccgagccg
     1801 ttcttcgtgt ccctttatct cgtcaagcgc cgtcagcagc gtccatgcct tgaccgtgcc
     1861 gccgtcatcg gcatcgggag tgagccgcag cacgccgctg ccgcggccct ccgccgtttc
     1921 gaagccaaag atggcttcga tcgcatcagt gccggcgcgc ctcacgttgc ggggcgcagt
     1981 gcgatgcggg tctatcttga aacccgccgg acgcgcgcgg ccggcatgcg ttgcaagctc
     2041 gcgcacaatc gcatccgagc cgctgacggt cttgaggtgc cacgtcaggg cgagcagatc
     2101 gcgccagtgg ctgtcagcat gaaacagccg ctcgaggcga gggcggcctg gcgctgcgag
     2161 cgctgcttcg aaccgcgcca gccaactctc cgcaatcgtt gcgacggtgt ctgctctggc
     2221 cagcattggc gctattccgg tggggtcggg gaaccgctgg ttcggggttg aaactagcat
     2281 atcaggaaga ccgtcgcgcg gcggtgggcg cagcgctaga atgggtcgtc aattaggagc
     2341 gacgatgcgc acgaagcgaa tgttcctcac cgcaatcgtg gccgtcggca tcggcctcgc
     2401 gggcgcgagt ctcgccagcg cccaatccta ccccgaccgg ctgatcaaga tcgtcgtgcc
     2461 gtatcctcct ggcggtcccg ccgacgtggc ggcgcgcctc gtcacccagc cgctctctac
     2521 gcgactgggg caaagcgtca tcgtcgaaaa tcagccgggc ggcggcggac ggaccggcgc
     2581 caagttcgtg gcgcacgcca gcgcggatgg ctacacgttg ctgctgggcg gcaccaaccc
     2641 caacgccatc gcctcgctct atcgcacgct cgacttcgaa ccgatcaagg acttcgccgg
     2701 cgtcggtctg atcgggttcg actccaatgc gctggtggtc aacccggcgg tcccggcgaa
     2761 aacgctgcag gaa
//
LOCUS       DATLJT010000133         1557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_465883,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000133 DATLJT010000000
VERSION     DATLJT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465883"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VM910_02905"
     CDS             complement(<1..546)
                     /locus_tag="VM910_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVI61535.1"
                     /translation="MLVVDVGGTSVKILATGQKEERSFPSGPTLTPRQMVSGVKKLAR
                     DWTYDVVSIGYPGPILHGCPIAEPYNLGRGWVGFDFAVAFGRPVKVVNDAAMQALGSY
                     NGGKMLFLGLGTGLGSTLIVNGIVEPMELGHLPYKKGTYEDYVGRAGLERHGKKKWRR
                     HVADVVARFIAALEPDDTVIGG"
     gene            complement(638..>1557)
                     /locus_tag="VM910_02910"
     CDS             complement(638..>1557)
                     /locus_tag="VM910_02910"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVI61536.1"
                     /translation="DYDDAIKSKLAEGKSGEKLFFELALEDLTQAADLFRPVYDRTCG
                     VDGWVSLEVSPLLAHDTKSTIAAAKDLHAQARRPNVFIKIPGTPEGLPAIEEAIFAGV
                     PINVTLLFSDDQYLAAANAFMRGIERRIEAGLKPDVASVASIFISRWDVAVTGKVPKA
                     LNDRLGIAIAKRTYKAYLALLSSPRWMRAYNAGARPQRLLWASTGTKDPEASDVLYVK
                     SLAAPFTVNTMPEGTLKALADHGDVGATIPADGGDSEKELARFAEAKIDVHALSKQLQ
                     DEGAKSFVKSWNDLMGVISSKSAVLKRAS"
BASE COUNT          276 a          496 c          474 g          311 t
ORIGIN      
        1 gcccccaatc accgtatcgt cgggctcgag cgcggcgatg aagcgggcca cgacgtcggc
       61 aacgtggcgg cgccatttct tcttgccgtg gcgctccagt ccggcacggc ccacgtagtc
      121 ctcataggta cccttcttgt aaggcaggtg accgagttcc atgggctcca cgatcccgtt
      181 cacaatcaga gtggatccaa gtcccgttcc gaggcctaga aagagcatct tgccgccatt
      241 gtagctccct agagcctgca tcgcggcatc gttgacgacc ttgaccgggc ggccgaacgc
      301 tactgcgaag tcgaagccga cccatccgcg cccgaggtta tatggttcgg caatcgggca
      361 gccgtgcagg atcggaccgg gatacccgat agataccacg tcatacgtcc aatctcgggc
      421 gagcttcttc accccggaca ccatctgcct tggggtcaac gtcggacccg atggaaatga
      481 gcgctcctcc ttttgtccgg tggcgaggat cttgaccgag gttccgccca catcgacgac
      541 caacacttgc ccggcggcgg acgccttcgc aacgtcagct cgatctagcc gccgtcgccg
      601 gcgcttggtg gtgggccgcg acgtcgcctt caccgcgcta gctcgctcgc ttgagtacgg
      661 ctgatttgga actgatcacg cccatcaggt cgttccacga cttgacgaag gacttggcac
      721 cttcgtcctg aagctgcttc gatagtgcat ggacgtcgat ctttgcctcc gcaaaccgcg
      781 ccagctcctt ttcggagtcg ccgccgtccg cgggaatggt tgcgccgaca tcgccgtggt
      841 cagcgagggc tttgagcgtg ccctcaggca tcgtgttcac cgtgaatggc gcggccaggc
      901 tcttgacgta gagaacatcc gacgcctccg ggtccttggt cccggtgctg gcccagagca
      961 ggcgctgcgg gcgcgcgcca gcattatagg cgcgcatcca ccgtggtgaa ctcagcaacg
     1021 cgagataggc cttgtaggtg cgcttggcga tagcgatacc gagccgatca ttgagtgcct
     1081 tcggtacttt gccggtgacc gcgacgtccc agcggctgat gaagatagat gctaccgatg
     1141 cgacatcggg cttcaatccc gcttcgatgc gccgttcgat gccgcgcatg aatgcgttgg
     1201 cggccgccag gtactggtcg tcggagaaca gcagcgtgac gttgatcggc acgccggcaa
     1261 agatcgcctc ttcgatggcc ggcaggcctt cgggggtgcc cgggatcttg atgaagacat
     1321 tgggacgccg cgcctgcgcg tgcaggtcct tggcggcggc aatcgtcgac ttggtgtcgt
     1381 gcgccagcaa aggcgagact tccagcgaaa cccaaccgtc gacaccgcag gttcggtcat
     1441 aaaccggccg gaacaaatcc gccgcttgcg taaggtcttc cagcgcgagt tcgaagaaaa
     1501 gcttttcccc cgacttacct tccgcgagct tgcttttaat ggcgtcgtcg tagtcgc
//
LOCUS       DATLJT010000134         1912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1614502,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000134 DATLJT010000000
VERSION     DATLJT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614502"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VM910_02915"
     CDS             <1..393
                     /locus_tag="VM910_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61537.1"
                     /translation="GNPASALEIGEVQAAARALGLEVTTLEIRRAEDIAPAFEAIEGR
                     ADAVYIPTEPLVLTNRVRINTMALGARLPTIYSGREYVESAGLMSYGPNRPDLFRRAG
                     DIADKILRGAKPGDIPVEQRPNSISSST"
     gene            660..>1912
                     /locus_tag="VM910_02920"
     CDS             660..>1912
                     /locus_tag="VM910_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI61538.1"
                     /translation="MSKRRNFPDSNRRNFLKGAGLVSAAALTPLTAGAQVSAPKQNLK
                     APVPGPTQIAVETLPPAKDPVNQTSSGGDFMVDVIKTLNFDYLAMNCASAFRGIHESF
                     INYGKNTKPEILTCPHEEIAVHMAQGYAKMEGKPMAMMCHGTVGLQHASMALYNAWCD
                     RVPVYVMIGNIIEGDKRAPGVEWVHSAIDPAAIVRDFVKWDDQPASLQHFAESAVRAY
                     KIAMTPPMAPVLLSLDAELQENPIVDPESLRIPKLANVVPPVGDAGALGDAAKMLVAA
                     ENPVIICDRLARSPAGMSRIVELAETLQCAVVDNAGRMNFPSRHPLNQSFRRSVISQA
                     DVILAIEMNDVFGSLNAFSDRIARATRPTVKKGAKIITLGLRDTYMKSNYQDFGRYQD
                     VDLAIAGDGEASLALLIEAVKRFIDP"
BASE COUNT          389 a          596 c          580 g          347 t
ORIGIN      
        1 ggcaatcccg cctccgctct ggagatcggc gaggttcaag cagcggccag agcgctcggc
       61 ctcgaggtca ccacactcga aattcggcga gccgaggata ttgcacctgc cttcgaggcg
      121 atcgagggtc gcgcggatgc agtttatatt cccaccgagc cgcttgtatt gaccaaccgg
      181 gttcgtatca acaccatggc gctgggcgcg cgcctgccga cgatctatag cggtcgggaa
      241 tatgtcgaat cggcaggtct aatgtcctat ggaccaaacc gtccggacct attccgccgc
      301 gccggcgaca ttgctgacaa gattctgcgt ggggcgaagc cgggcgacat cccagtcgag
      361 cagcggccaa attcgatctc gtcatcaacc tgacgaccgc caaggccctc cacctcaaag
      421 ttccggagtc gttcctgttg cgcgccgacg aggtgatcga atgagcagcc gtcgcgagtt
      481 catcacgctg ctcggcggcg cggcggccgc gtggccgctc gttgtgcgcg cacagcagcg
      541 catgcagctg tcaccggatg gcaccgtgcc ttgacgggag cagggggcgg cgcgacactg
      601 acaggctgac tttgcgcgaa ctgccgagcc cgcgtccacc ctatggatgg gagaagtgca
      661 tgtcgaaacg ccgcaatttt cccgattcaa atcgccgcaa ctttctcaag ggggccggcc
      721 tcgtcagcgc cgcggcgcta acgccgctca cggccggcgc gcaggtttcc gcgccaaagc
      781 aaaacctcaa ggcccccgtt cctgggccga cgcaaatcgc ggtcgagacc ctgccgcccg
      841 cgaaggaccc ggtgaatcag acgtcgagcg gcggcgattt catggtcgat gtgatcaaga
      901 cgctcaattt cgactatctg gcgatgaact gcgcctcggc gttccgcggc atccatgaat
      961 cctttatcaa ttatggcaag aataccaagc ccgaaatcct cacctgccca catgaagaga
     1021 tcgccgtcca catggcgcag ggctacgcca agatggaagg caaaccgatg gcgatgatgt
     1081 gtcacggcac cgtgggcctg cagcacgcaa gcatggcgtt gtacaacgca tggtgcgacc
     1141 gggtgccggt ctatgtcatg atcggcaaca taatcgaggg ggacaagcga gctcccggcg
     1201 ttgaatgggt gcactcagcg attgacccgg ccgcgatcgt gcgcgatttc gtcaaatggg
     1261 acgaccagcc ggcgtctctc cagcactttg ctgagtcggc agtgcgcgcc tacaagatcg
     1321 cgatgacgcc gccgatggca ccggtcttac tctcgctcga cgccgagctg caggaaaacc
     1381 cgatcgtcga cccggaaagt ctgcgcatcc cgaagcttgc caatgtcgta cctcccgtgg
     1441 gagatgcggg cgcgctcggg gacgcggcaa agatgctggt ggcagccgag aatcccgtga
     1501 tcatctgcga ccggctcgcg cgcagcccag ccggcatgtc gcgtatcgtc gagctagccg
     1561 agacgctgca atgcgcggtg gtcgataatg ctggccggat gaactttccc tcgcgccatc
     1621 cactcaatca atcatttcgc agatccgtca tttcgcaagc cgacgtgatt cttgcgatcg
     1681 agatgaacga cgtatttgga agcctgaacg cgttcagcga tcgcatcgct cgggcgacgc
     1741 gacccaccgt caagaagggc gccaaaatca tcacgttagg gctccgcgac acgtatatga
     1801 agtccaatta tcaggacttc ggacgctatc aggatgtcga cctcgcgatt gcgggtgacg
     1861 gtgaggcaag ccttgcgctg ctgatcgagg cggtgaaacg cttcatcgat cc
//
LOCUS       DATLJT010000135         3152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_695269,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000135 DATLJT010000000
VERSION     DATLJT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695269"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VM910_02925"
     CDS             complement(<1..1130)
                     /locus_tag="VM910_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61539.1"
                     /translation="MRSDAAVPEAAQGAVAGAPSAWPARLFGRSRPKQCSQNIQKNPC
                     WTTVAEPIWGTLAELPFTGDQVVCLGHNQAAVGHTMRTQPDYSFVALTARVFPRLPER
                     AQNVLKSRIAGALKDNVGLSPFAFEMRTVAHLMASGFDVEFHDLCEGRGFDFLAKSGD
                     ITFEVECKSVSGDLGHRVHTYRQYQLMPYVLDKIQSSEKSGIVRLLVATVPDRLYGQQ
                     EFMKAVAATIGKGLEKSADVSSDACTMGYYELPILGSPFDCESPPRIKEDDVVDYCNH
                     VIGDEVGHTIMTFSPRQSATIIAVRSLKPNRPLNGVYRSLTEAARQLSGSRPGMICVQ
                     FRNMTSAELRDLAEHPAQSGQPTGIQLMTAKFYDDSTHGHVHT"
     gene            1260..2231
                     /locus_tag="VM910_02930"
     CDS             1260..2231
                     /locus_tag="VM910_02930"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HVI61540.1"
                     /translation="MAALLLLASLAPVAAVAQQTPQPVFTELFAYDPAAPSQLLRKWR
                     ISSQLDSKLQPLRVGVVEDLSGKRVGRVTVQEGDGLDDASEAMLQARRYICNSDGSRA
                     AEVEDEPGGVVPSERAEIQVRSDRATGAGELVKFGQPVWYRFSFKTAGDWPRDVPAAG
                     RQPCRTVIHQIKQDSFKGGKSCNASPFFKIEARPLGERVLFFAQVAAGSPCAQSPMVM
                     RAQICRRDLPRESWTTVQMRLNPAHDASGRVDIWLNGAFCGTYQGPTADRNYGARRNG
                     TPFVNAQPRFGIYRDWRAETQTIYFDKIMFWNTDPAGHPDWGVNPSG"
BASE COUNT          614 a          990 c          901 g          647 t
ORIGIN      
        1 gtgtgcacgt gaccgtgagt ggagtcgtca tagaattttg cggtcatcag ttggatgccc
       61 gtaggttgtc cggactgcgc ggggtgctca gcaaggtctc gaagctctgc gctcgtcata
      121 tttctgaact gcacgcaaat cattcccggc cgcgagccgg atagttgacg ggccgcttct
      181 gttaaagagc gataaacccc attcagtggg cgatttggct tcaggctgcg tactgcaatt
      241 atcgttgcgc tctgtcgcgg actaaaggtc ataatcgtgt gaccgacttc atcaccgatc
      301 acgtggttgc agtaatcaac tacgtcatct tctttgatgc gcggcggcga ctcacagtcg
      361 aacggtgagc cgaggatcgg cagctcgtaa tagcccatag tacacgcatc gctactaacg
      421 tcggctgatt tttccagccc tttcccaatt gttgccgcga cagccttcat aaattcctgt
      481 tgtccataga gtcggtcggg aaccgttgcg accaatagtc gtacgatgcc gcttttttct
      541 gagctctgta ttttgtctaa gacataaggc ataagttggt attgtcgata cgtgtgtact
      601 cgatggccaa gatcaccgct aactgacttg cattccactt caaaggtaat atctccggat
      661 tttgctagaa agtcgaaacc tctaccttca cacaagtcgt gaaactcaac atcgaaaccg
      721 cttgccatga gatgcgctac agtcctcatc tcgaatgcaa atggtgatag tcccacatta
      781 tctttgagcg ccccggcgat tctacttttc agaacgtttt gagcgcgctc gggtaaccta
      841 ggaaaaacac gcgctgtcaa tgctacgaac gagtaatcgg gctgagttcg catcgtatgc
      901 cccacggcgg cctggttgtg gcctaaacag acgacctgat ccccggtaaa gggcagttcc
      961 gctagtgttc cccaaatcgg ctcagctacg gttgtccagc aaggattttt ctggatattt
     1021 tggctgcact gttttggcct gctccgaccg aataggcgcg caggccacgc cgacggtgcg
     1081 cccgccaccg ctccctgagc cgcctccggc accgctgcat cagagcgcaa gaccccatcg
     1141 gcctttttcc tctaccatca tcgtagcgcc gcgtggcaat cgaggctccc accatggtgc
     1201 gaccgggcaa agtagcggct tcccgcgggt cgcgtcccag gctcgacgcc gggcgcagct
     1261 tggcggcatt gctgctcctc gcttcactcg cgccggtggc ggcggtcgcg cagcagacgc
     1321 cacaacctgt cttcaccgag ctgttcgctt acgatccggc tgcgccgtcg cagttactga
     1381 ggaaatggcg gatctccagt cagctcgaca gcaagctgca gccgctgcgc gtcggcgtcg
     1441 tcgaggacct cagcggcaag agggtcggcc gcgtcacggt gcaggagggc gatgggcttg
     1501 acgacgcgag cgaggcgatg ctgcaggcca ggcgctacat ctgcaatagc gacggctcgc
     1561 gcgcggcgga ggtggaggac gagcctggcg gcgtcgtgcc aagcgagcgg gcggagatcc
     1621 aggtgaggtc cgaccgcgcg accggcgccg gcgagctggt gaagttcggt cagcccgtgt
     1681 ggtaccgctt ctccttcaag accgccggcg actggccgcg ggacgttccg gcggcgggac
     1741 gccagccctg ccgcactgtc atccaccaga tcaagcagga ttccttcaag ggcgggaagt
     1801 cgtgcaacgc cagccccttc ttcaagatcg aggcgcgtcc tctcggcgag cgcgtcctct
     1861 tcttcgcgca ggtggccgcc ggctcaccct gcgcccagtc gccgatggtg atgcgcgcgc
     1921 agatctgccg gcgcgacctg ccgcgagaga gctggaccac ggtgcaaatg cgcctcaatc
     1981 ccgcccatga cgccagcggc cgcgtcgaca tctggctcaa cggcgcgttc tgcggcacct
     2041 accaggggcc cacggccgat cgcaactatg gtgcccgccg gaacggcacg cccttcgtca
     2101 acgcacagcc gcgcttcggc atctaccgcg actggcgcgc ggagacccag acgatctatt
     2161 tcgacaaaat catgttctgg aacacggacc cggcgggaca cccggactgg ggcgtgaatc
     2221 cgtcgggatg agtgccgatg cggccgagac ctcggcgggc atgggtcagg ctgccggtga
     2281 ccctactgcg ttcaagcagg cggtgtgaac cgaacatctt cgatatgtga gcgcagcaaa
     2341 cgacagaggc cttttgcacc acccgatagt cggccacatc agccgcctgt aattgacggg
     2401 ccgccgctcc gcacacagcg atgttgaagg cgccctaaat ggcagccaac atatccacaa
     2461 aaatcgttgt cggacaaccg tagctgatcc gattggggaa atcctagcgg aaccgcccat
     2521 taccggggat caggtcgtct gtttaggccg cgaccagggc cgcctggagc ggacctgcaa
     2581 ctaccccccc ttggttcggt gcgggttgca caattggcgg tttctcaccc atcgcctcag
     2641 aaaacctaac ccgccaatcg ccgcgtcgtg aagttctcct cgctcctgct catcgcccca
     2701 gatgcatctc ctcgcgcagt ccgcaattcg ccgcatcgat aagaccaaat tgaaactctg
     2761 gcttgtcatc tccgaatccc gacctcagtc ggtattcaag atcggcggaa tctcgaaatg
     2821 tccgccggcc cggacgacgg catcacggcc aagcgcaata gagcaaaaat cctcggcggc
     2881 atgtcgcatt cctgctgtcg cctgtcaggg cgtgacacgt gaggcagagc ccccaccttt
     2941 ggcgcggctt cgtcccgcca aacctgtttg tggttacagt tgccttctgg gcttctggaa
     3001 gagcccggct gcccaatctg cgaaggcttt gtcccacaca tcgttcgatt cggaagcacc
     3061 gctaaagcct cgctcttccc aaaatctggc ccaatcgtcc gcatccattt tgtgctcagg
     3121 atcgatcaga tcttgtagac cctgtgcttc ga
//
LOCUS       DATLJT010000136         8968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2763629,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000136 DATLJT010000000
VERSION     DATLJT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763629"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..367)
                     /locus_tag="VM910_02935"
     CDS             complement(137..367)
                     /locus_tag="VM910_02935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HVI61541.1"
                     /translation="MDPTAAKYIGAGIACIGMGGAGVGVGIIFGNYLAAAIRNPSAAA
                     GQFANLIFGFAITEALGIFSLLIALLLLFALG"
     gene            complement(405..1151)
                     /locus_tag="VM910_02940"
     CDS             complement(405..1151)
                     /locus_tag="VM910_02940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVI61542.1"
                     /translation="MDPIHQFEIKNFFPIVHIGGVEIAFTNSALFMLIALALILALML
                     GATRSRALVPGRLQAVAEMSYEFVADTLRSSAGPEGMKFFPLVFSIFMFILVLNVIGI
                     IPYAFTVTSHIIITVAFALLVFLTVMIYGFWRHGLRFLKLFVPSGIPIYILPLVTAIE
                     VLSFFSRPVSHSVRLFANMLAGHITLKVFGGFVVMLAGFGFLGWLGAALPLALTTVLT
                     ALELLVALLQAYVFAILTCIYLNDAVHPGH"
     gene            complement(1214..1591)
                     /locus_tag="VM910_02945"
     CDS             complement(1214..1591)
                     /locus_tag="VM910_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVI61543.1"
                     /translation="MGDDAREKGDGEDLPADEAALSARFKRLGERLGRVHADRPSESS
                     SDQRPAADPSAIARGFRLSTEFVAGVVVGAAIGWLLDRWLGISPWGMIVFLLLGFAAG
                     VLNVMRAAGVVASNTLDPPGSKQ"
     gene            1930..2394
                     /locus_tag="VM910_02950"
     CDS             1930..2394
                     /locus_tag="VM910_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61544.1"
                     /translation="MIRTALFMVAGLALAGPAAWAQSAAPESDDSRYTFNRVDDGYLR
                     LDGRTGQVSICARRPAGWTCQVVPDERAALEGEIARLQGENIAIKKELLARNLPLPGT
                     VKPEPPAAKPEEPRLQLPNDADLDKVMNFIEKIWRRLVEMIATLQKDMLKKT"
     gene            2420..2881
                     /locus_tag="VM910_02955"
     CDS             2420..2881
                     /locus_tag="VM910_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVI61545.1"
                     /translation="MNDVLSPITSFASETIATATLVVETSGQAFTEITRAAMRFIKQA
                     GAKDGALFIFMRHTSASLVIQENADPDVRTDLASALDRLAPAHAQWVHDVEGPDDMPA
                     HVKTMLTGVSLHVPVIAGALALGVWQGIYIAEHRARPHRREIVLQFIGSRQ"
     gene            complement(2956..3138)
                     /locus_tag="VM910_02960"
     CDS             complement(2956..3138)
                     /locus_tag="VM910_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61546.1"
                     /translation="MIQIYGFQAMKRSKLQDMRVKRSWSVSPPLESKQDDTAKFSRLF
                     NEMEAVKRDLKSTPWR"
     gene            complement(3138..3317)
                     /locus_tag="VM910_02965"
     CDS             complement(3138..3317)
                     /locus_tag="VM910_02965"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HVI61547.1"
                     /translation="MNQLVERFTAITLDQDKALLRNEYAKFNRLFEQMEDVKRELKAR
                     DGDQRRALLPLYEPT"
     gene            complement(3453..3818)
                     /locus_tag="VM910_02970"
     CDS             complement(3453..3818)
                     /locus_tag="VM910_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61548.1"
                     /translation="MEAGCPICAEWVPSAPSPPPRNPTQDQRWLAQEQGHVVEGADWF
                     SEYEPSVQAYLDSPNTLQELQQAVSAPKPGYDIHHIVEKTSAEQDGFPKLMINGSENL
                     VRIPRFKHREITGGLYDEE"
     gene            4208..4879
                     /locus_tag="VM910_02975"
     CDS             4208..4879
                     /locus_tag="VM910_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI61549.1"
                     /translation="MDESVHLIIADDHPLFRGALREAVSGLFEEVEIAEAGSFDDVAT
                     LLERGGEVDLILLDLTMPGVRGFSGLMYLRAQYPSVPIVVVSANDDPGVIRRCMDFGA
                     LGFIPKTLGIEAMRGAISRVFEGGVWTPPDVDLGAGSNAETAELLTRLTSLTPQQVRV
                     LMMLSEGLLNKQIAYELGVSEATVKAHVSAILQKLGVESRTQAVIAASRIELGQWPQT
                     VSASH"
     gene            complement(4919..8440)
                     /locus_tag="VM910_02980"
     CDS             complement(4919..8440)
                     /locus_tag="VM910_02980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HVI61550.1"
                     /translation="MLQGWIVIAFALGYIGLLFVVASYGDRTRQFGREGGWRHFIYPL
                     SLAIYCTSWTFFGSVGLASRTGFDFLTIYVGPILLVGLCSPLIMRIVRLAKAQNITSI
                     ADFIAARYGKAQTVAATVALIAIIGTIPYIALQLKAVSSSLTTILVHIGPASGAMQPV
                     LGDIALYVALAMAIFAVLFGTRHIDATEHQDGLMLAIATESIVKLVAFVGVGVFVTFW
                     MFDGPRLLFAQALERPDTAAVLTREPALGTLLAMTLLSLFAIILLPRQFHVTVVENNS
                     EDEIRRASWLFPLYLVLINLFVVPIALAGLLTFAPGQVDSDMFVLALPLAAHSDLFTI
                     AAFVGGLSAATAMVIVESVALAIMVSNDIVMPLVLQRREALITGRDNIGGLLLIVRRM
                     AIFVILFLAYVYYRLAGDAQLASIGLLSFAAVAQLAPAFFGGLIWRRATARGALAGMT
                     VGILAWAYTLLLPSLADAGIVGQSLLSQGPWGIALLRPQALIGLDLPPLAHGVIWSLS
                     LNALAYAVFSLGRAPASIERLQADLFVPSDLTPIPPSFRLWRSSVTVEELTATVSRYL
                     GEERTRTSFDSYALTRGISLDPKAEADFQLLRYAEHLLASAIGAASSRLVLSLLLRKR
                     TVSTKAALKLLDDANAAIHYNREILQTALDHVRQGIAVFDKELHLVCWNRQFGEILAL
                     PPQLTRFGTGLDHILRFHAEQITSDKNAADALVGERLARYVASSEPFLERFNERNLVM
                     EVRANPMPDGGIVTTFTDITPSVKAAEELERANESLELRVQERTEELTRLNTELGRAK
                     GEAEEANVSKTRFLAAASHDILQPLNAARLYVTSLVERQRSGNDAQLVTNIDASLDAV
                     EEIFGALLDISRLDTGAMKPEIVGFRIDELLRQLEVEFTPLAQEKGLTLKFVPCSLAV
                     QSDRRLLRRLLQNLVSNAIKYTPKGRVLVGCRPRGGRLRIDVHDTGLGIPASKKRAIF
                     QEFYRLDQGAKAARGLGLGLSIVERIARVLDCRVSVTSTVGRGSQFSVDVPLSAVIPA
                     RQHQRTARDVDRVQLSGITVLCIDNDLAILDGMETLLAGWGCRVFKAPDLKTAIAVVA
                     EAKIAPDGLLVDYHLDEGNGIAAIGELRRRFGADLTAILITADRSPHVREDARANAIQ
                     LLHKPIKPAALRALLTQWRMHRVAAAE"
     gene            8678..>8968
                     /locus_tag="VM910_02985"
     CDS             8678..>8968
                     /locus_tag="VM910_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61551.1"
                     /translation="MPPPVSPLRELMQVVGTLLIAAVGGIGFHLVGFPGGLVSGSMLT
                     VAVAALAGCAVKIPLSLARLCFVLVGILLGAVVTPDTLKGIASWPLSVALLVV"
BASE COUNT         1777 a         2702 c         2857 g         1632 t
ORIGIN      
        1 ttgaagggcg gaaacccggc accgtgctcg cttgcgggaa cctcgacgga tcctaccggc
       61 tctacactac cttgggccat tgccgcctcc atgggctgcg gcgcggtcgg ctaccgccca
      121 cgcgttcgat gcttggtcaa ccgagggcaa acagcaaaag cagcgcaatc agtagcgaga
      181 agatgccaag cgcctcggtg atggcgaagc cgaaaatcaa gttggcgaac tgtccggccg
      241 cggcagacgg gttccggatc gccgcagcca aatagttgcc gaagatgatg ccgacgccga
      301 cgccggcacc gcccatgccg atgcaggcaa ttccagcacc aatatatttc gcggccgtcg
      361 ggtccatgag aaagctcctg ttcgagggtt gcttgaagga tcggtcaatg accaggatga
      421 accgcatcgt tgaggtagat gcaagtcagg atggcgaaaa cgtaggcctg caacaaagcc
      481 accagcagct cgagcgccgt gagcaccgtc gtcagagcca gcggcaaggc ggcgccaagc
      541 cagcctagaa atccaaaacc cgcgagcatc acgacgaacc cgccgaacac cttcaaggtg
      601 atgtgaccgg cgagcatgtt ggcgaacagc cgtacgctat gggagaccgg ccgcgagaag
      661 aatgacaaca cctcgatcgc cgtcaccagc ggcaggatgt agatgggtat cccgcttggc
      721 acgaacagct tgaggaagcg caatccgtgc cgccaaaagc cgtagatcat gaccgtgagg
      781 aagaccagca gcgcgaacgc aacggtaatg atgatgtggc tcgtgactgt gaaggcatat
      841 ggaataatac ctatcacgtt gagcaccaga atgaacatga agatcgagaa taccagcgga
      901 aagaatttca tgccttccgg gccggcggaa cttcgcagtg tgtccgcgac gaactcgtaa
      961 gacatttcag cgaccgcctg cagccttccg gggactaggg cgcgcgagcg ggtggcgccg
     1021 agcatcagcg caaggatcaa ggcgagcgcg atcagcatga aaagcgccga attcgtgaag
     1081 gcaatctcga ccccgccgat gtggacgatc ggaaagaagt ttttgatttc gaactgatgg
     1141 atgggatcca cgacggatca agtccttcat cgctgcgtgg ccgcctcgcc gcttcgcgcg
     1201 ggggtggccg aattcattgc ttcgacccgg gcgggtccaa tgtgttgctc gctacgactc
     1261 ccgccgcgcg catcacgttg agcaccccgg ctgcgaagcc gagtagcaga aacacgatca
     1321 tcccccaagg cgaaattccg agccagcgat caagcagcca gccgatggct gcaccgacca
     1381 caaccccggc cacgaactcg gtcgagagcc gaaaaccgcg ggcgatcgct gacggatcgg
     1441 cggccggacg ttgatccgaa ctgctctcgg aaggacggtc agcatggacg cggccgagcc
     1501 gttcgccgag gcgcttaaac ctcgctgaca gcgcagcttc gtccgccggg aggtcttcgc
     1561 catcaccttt ctcgcgtgcg tcgtcgccca tgctttcgac acccgcgacg gttcaggcaa
     1621 gatcacgata attccgcccc caaagtcgcg gggaccatac tgactgggtc tatgcgagtc
     1681 aagaattgcg ccttcgcctt aaatagttga caatgaattg gaatcacgcg gtcggcgacg
     1741 gatggaaccg gcgtttttcg acaatctcgg ggcgtggact ttgaacccaa ggcaacgtcc
     1801 tttgcatttc ctttactgga cggcgcggtg gtgacttttc ctgatctggc tcgcgctcag
     1861 tctgcacagt gggtgccatc gcatcgcccc agagcggttg tctctgcacg acgcgcaagg
     1921 agggagcgca tgatccgcac ggctctattc atggtcgccg gacttgcgct cgcggggccg
     1981 gcggcttggg cgcaaagcgc ggcgccggag agcgacgaca gccgctacac cttcaaccgc
     2041 gtcgatgacg ggtatcttcg gcttgacggc cgtaccggcc aagtatcgat ctgcgcgcgg
     2101 cgcccagctg gatggacctg ccaggtggtg ccggacgagc gcgcggcgct cgagggcgag
     2161 atcgcgcgcc ttcagggcga aaacatagcg atcaagaaag agctgcttgc gcgcaacctg
     2221 ccgctgcctg gcaccgtcaa gccggagccg ccggcggcca agccggagga gcctcgcctg
     2281 caattgccca acgatgccga tctcgacaag gtcatgaatt tcatcgagaa gatctggcgc
     2341 cgcctggttg agatgatcgc gacgctacag aaggacatgc tgaaaaagac ctgacgatcg
     2401 ccgcgatggc gaggcagcca tgaatgatgt gttgtctcca attaccagct tcgcgtccga
     2461 aaccatcgcg actgcgacgc tcgtggtcga gaccagcgga caggccttca ccgagatcac
     2521 gcgcgcggcg atgcgattca tcaagcaggc cggggcgaag gacggcgcgc tgttcatctt
     2581 catgcggcat acttcggcct cgctcgtcat ccaggaaaac gccgatcccg atgtacgcac
     2641 cgaccttgcg agcgcgctcg atcgcctcgc gccggcccac gcccagtggg ttcatgatgt
     2701 cgaggggcct gacgacatgc cggcgcatgt caagaccatg ctcacgggcg tgtcgctcca
     2761 cgttcccgtg attgccgggg cgctcgcgct cggggtctgg caaggcatct acatcgccga
     2821 gcatcgcgcc cggccgcacc ggcgggaaat cgtgctgcaa ttcatcggca gcaggcagta
     2881 atcccggttg tctggcgttc tcacgaacgc tcgagcgaag ctaaccgatg gcaggccgtg
     2941 aacacgatgc ggaagtcatc gccacggcgt actttttaag tcccgcttca cggcctccat
     3001 ttcattaaaa aggcgactaa atttcgcggt gtcgtcttgt tttgattcca atggcggtga
     3061 aacgctccat gagcgcttca ccctcatgtc ctggagtttg ctccgcttca tggcttgaaa
     3121 gccgtaaatc tggatcatca ggtcggctcg tagagcggaa gcaatgcccg tcgttggtca
     3181 ccatcgcgtg ccttcaattc cctcttcaca tcctccattt gctcgaacag acgattgaac
     3241 ttcgcgtatt cgttcctcag caacgccttg tcctggtcga gtgtaatggc ggtaaaccgc
     3301 tccacgagct gattcaccgt gatatcttgg agttcggccc gcttcatagt tttaggaccc
     3361 cgtgctggat cagagctcag agaccaactc tcgtccgttc atcccagtct ttgccgcgta
     3421 ggtaatctct gggcggaagt ccgtcgtagt ctttattcct cgtcatataa ccccccggtg
     3481 atctcccggt gtttgaacct tggtatccgc accagatttt cggaaccatt gatcatcaat
     3541 ttcggaaaac cgtcctgctc ggccgaggtt ttctccacga tgtggtgaat atcatatccc
     3601 ggttttggcg ctgaaacagc ctgctgcagt tcctgcaggg tgttgggcga atcgagatag
     3661 gcttggaccg acggctcgta ttcggaaaac cagtcggctc cttcgacgac atgcccttgc
     3721 tcttgagcga gccatcgctg atcctgcgtg ggattgcgcg gaggggggga gggcgcggag
     3781 gggacccact ctgcgcatat cgggcacccg gcctccaacc gttgtccggt gtgatgtcgg
     3841 agatgactcg cggatcgctt tcggcgccgg tgtctccgct catccactgc caataggcag
     3901 ggcgccccgt cgttacgacc ggcccccacg tccgagaagc caagctgctc tgcgccggcg
     3961 gatgccgtcg aacgattcgg ctgccagtta tcgttcagca tatcgccgag ttgcttcgcg
     4021 ccccttgatt gctagtcgcg ctcgggatct ggcagggcat gtatgttgcc gagcatcgcg
     4081 cccggccgca ccgccgagcg aagccaaccg atggcaggcc gtgaacacga tcccgaagtc
     4141 gtgctatgct atcggcgctg gcgacgtgtg ggccccaagc acaaaggccg cgcgggagat
     4201 gaagcgggtg gacgaatcgg tacacttgat catcgcggac gaccatccgc tgtttcgcgg
     4261 ggctttgcgc gaggccgtct cgggtctgtt cgaagaggtc gagattgccg aggcaggatc
     4321 cttcgatgat gtggcgacgc tgctcgagcg cggtggggag gttgatctca ttctgctcga
     4381 cttgacgatg cccggcgtgc gcggattctc gggcctcatg tatctacgcg ctcagtatcc
     4441 aagcgtcccg attgtcgtgg tatcggccaa cgatgatccc ggggtcatcc gccggtgcat
     4501 ggacttcggc gccttgggtt tcatccccaa gacgctcggc atcgaagcga tgcgaggggc
     4561 gattagccgg gtgttcgagg gcggcgtttg gaccccgccc gatgtcgacc tcggggccgg
     4621 ctcgaatgcc gagacggccg agttgctgac acggcttacg tcattgacgc cccagcaggt
     4681 acgcgtgctg atgatgctgt cggaaggcct gctcaataaa cagattgcct acgaacttgg
     4741 cgtctccgaa gcgaccgtca aggcgcacgt ctcggcgatt ctgcaaaagc tcggggtcga
     4801 gagccgcaca caagcggtga tcgccgcctc caggatcgag cttggccagt ggccgcagac
     4861 ggtaagcgcg agccactgat ttgttgaatt gatggtaatt cgtcgatcgt gggccgcctc
     4921 attctgcggc cgcgacgcgg tgcatgcgcc actgcgtcaa tagcgcgcgc aatgctgccg
     4981 gcttgatcgg cttgtggagc agctggatgg cattcgcgcg ggcgtcctca cgcacgtgcg
     5041 ggctgcgatc ggcggtgatc aggatagccg tgaggtcggc gccgaagcgc cggcgcagct
     5101 ccccgatcgc cgcaatcccg tttccttcgt cgaggtggta atccaccagc agcccgtcag
     5161 gcgctatctt ggcttctgcg acgaccgcga ttgctgtctt gaggtcgggc gccttgaaga
     5221 cccggcagcc ccatccagcg agcagcgttt ccattccatc gagaatcgca aggtcattgt
     5281 cgatgcacag cacagtgatc ccggagagct gcacgcgatc tacgtctcgg gccgtacgtt
     5341 gatgctgcct tgcgggaata acggcggaga gcggcacgtc gaccgagaac tgcgacccac
     5401 ggccgaccgt tgaagtgacg ctgaccctac agtcgagaac gcgtgcgatg cgctcaacga
     5461 tcgaaagccc aagccccaag ccgcgtgccg ccttggcacc ctgatcgagt cggtaaaatt
     5521 cctgaaaaat tgcacgcttt ttcgaagccg gaatgccaag tccggtatcg tggacgtcga
     5581 tgcgcagtcg tccgccgcgc ggtcgacagc cgacaagcac gcggcccttg ggggtgtatt
     5641 tgatcgcgtt ggagacgagg ttctgtagca accggcgcaa caaacgccgg tcggactgca
     5701 cagcgagcga gcagggcacg aatttgagcg ttagtccctt ctcctgcgcg agtggtgtga
     5761 attcgacctc gagctggcgc aaaagctcgt cgatgcgaaa gccgacgatc tccggcttca
     5821 ttgcgccggt atcgagccga gaaatgtcga gcagcgcgcc gaatatttct tcgacggcgt
     5881 cgagcgaggc gtcgatatta gtgaccagct gggcatcatt gccgctccgt tgccgctcga
     5941 ccaggctggt gacgtagagg cgggcggcat tgagcggttg cagaatgtcg tggcttgcgg
     6001 cggcgaggaa gcgcgtcttc gaaacgttgg cctcttcagc ctcgcccttg gcacggccga
     6061 gttcagtatt gaggcgggtc aattcttcgg tgcgctcctg cacccgcagc tccagcgatt
     6121 cgttggcgcg ctcaagctcc tccgccgcct tcacgctcgg ggtaatgtcg gtgaaggtcg
     6181 tgacgatgcc gccgtccggc atggggttcg cacgcacctc catcaccaga ttgcgctcgt
     6241 taaaacgttc cagaaacggc tcgctgcttg cgacgtaacg ggcaagccgt tcgcccacga
     6301 gtgcatcggc ggcgttcttg tcagaagtga tctgctcggc gtggaagcgc aggatgtggt
     6361 cgagcccggt accgaaccga gtgagctgcg gcggcagcgc gaggatctcg ccgaattggc
     6421 gattccagca gacgaggtgc aactccttgt cgaatactgc gatgccctgg cgaacatggt
     6481 cgagcgcggt ctgtaaaatc tcccgattgt aatggatggc ggcgttggcg tcgtcgagaa
     6541 gcttgagcgc ggccttggtc gagaccgtgc gcttgcgcag gagaagcgag agcacgaggc
     6601 gggacgatgc cgcgccgatc gcggacgcga gcagatgctc ggcgtagcga aggagctgga
     6661 agtccgcctc ggcctttgga tcgaggctga tgccgcgggt caaggcatag ctgtcgaagg
     6721 aggtgcgcgt gcgctcctcg ccgaggtagc gggagacggt cgcggtcagc tcctcgaccg
     6781 ttaccgaaga gcgccacagc cgaaagcttg gcgggatggg ggtgaggtcg gaggggacga
     6841 agaggtcggc ctgcagtcgt tcgatcgagg ccggcgcccg tccaagcgag aacacggcat
     6901 aggcaagcgc attgagcgaa aggctccaga ttacgccgtg ggcgagcggc ggcagatcga
     6961 gcccgatgag cgcctgcggg cgcagcagcg cgattcccca cgggccctgg gagagcaggc
     7021 tctgaccgac gatgccggcg tccgccaagc tcggcagcag cagcgtgtag gcccaggcca
     7081 ggattccgac ggtcatcccc gccaacgcgc cgcgtgcggt agcccgtcgc cagatcagtc
     7141 cgccgaaaaa ggcaggcgcg agctgcgcaa ccgccgcaaa ggacaacaag ccgatcgagg
     7201 caagctgcgc atcgcccgcc aaacgatagt atacgtaggc gagaaacaaa ataacgaaga
     7261 tcgccattcg ccgcacgatg aggagcaacc caccgatatt gtctcgaccg gtgatcagtg
     7321 cctcgcggcg ctgcagcacg agcggcatga cgatgtcgtt cgacaccata atggcgagcg
     7381 caaccgattc cacgatcacc atcgcggtcg ccgccgagag gcccccgacg aaggctgcga
     7441 tcgtaaacag gtcggaatgc gcggcgagcg gtagggccag gacgaacatg tcgctgtcca
     7501 cctgccccgg cgcgaacgtt agcaggccgg ccaaggcgat cggtacgacg aacagattga
     7561 tcagtacgag atagagcggg aacagccagg aagcgcgcct gatctcgtct tcgctgttgt
     7621 tctcgaccac ggtaacgtgg aactgacgcg gcagcagtat tatcgcgaac agcgacagca
     7681 gcgtcattgc gagcagcgtg cccaacgccg gctcgcgggt gagcacggcc gcggtgtccg
     7741 gccgctccag ggcttgggcg aacagtagcc gcggcccgtc gaacatccag aacgtgacga
     7801 atacgccgac gccgacgaaa gcaacaagct tgacgatcga ctcggttgcg atcgccagca
     7861 tcaatccatc ctgatgctcg gtggcatcga tatggcgcgt accaaatagg acggcgaaga
     7921 tggccattgc cagcgccacg tagagcgcga tgtcgcccaa taccggctgc atcgcgccgc
     7981 tcgcgggtcc gatatgcacg aggatcgtgg tcagcgaaga cgacaccgct ttgagctgaa
     8041 gcgcgatgta gggaatggtg ccgatgatgg cgatgagcgc cacggtggcg gcgacggtct
     8101 gggccttgcc gtaacgggcg gcaatgaagt ccgcaatcga cgtgatgttc tgcgccttgg
     8161 ccagccgcac gatgcgcatg atcagcggcg agcacaaccc gaccaacagg atcggcccga
     8221 catagatggt cagaaagtcg aagccggtgc gcgatgcgag ccccaccgag ccaaagaacg
     8281 tccaggaggt gcagtagatc gcgagcgaga gcggatagat gaagtgccgc caaccgcctt
     8341 cgcgcccgaa ctgccgagtc cggtccccgt agctcgcaac cacgaacaag aggccgatat
     8401 agccgagcgc gaaggcgata acgatccagc cttgcagcat aatggttccg cggtccttgg
     8461 gcgcgaagcc cgcggatgca ttctaccgca cgaatgggca agcgagaata tcgctgcgcg
     8521 cgtggtggcg ctcccctcgg cacgtcaagg gagcttggat ccgcctcggc actttggccg
     8581 cgatttgacg cagtgcaccg caggcggcat tgatcggtga ttaagagcgc accgcgggac
     8641 gcgccgttcc cggcgacccg agcgtccggg accatcgatg ccgcctcctg tttccccact
     8701 gcgcgaactt atgcaggttg tggggacgtt gctgatcgcc gcggtgggcg ggataggctt
     8761 ccatctcgtc ggctttccgg gcggactggt ttcgggctca atgctaacgg tcgcggtcgc
     8821 cgcgctagcg ggctgtgccg tgaagatacc gctgtctctc gcgcgcttgt gcttcgtgct
     8881 ggtcggtatt ctgctggggg cggtggtcac accagacacg ctcaagggga ttgcgagctg
     8941 gccgctgagc gtcgcgctgc tcgtcgtc
//
LOCUS       DATLJT010000137         1718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_465990,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000137 DATLJT010000000
VERSION     DATLJT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465990"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VM910_02990"
     CDS             complement(<1..470)
                     /locus_tag="VM910_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61552.1"
                     /translation="MNTQLDALPESSDGSLAIAPAEIPALRHTYWALRRELWENRAIY
                     VAPLAVAAVILFGSLISTIGLPHRMRALSALDPARQRHAVASHYDLAAALLMGTFLIV
                     SVFYCIEALQRERRDRSILFWKSLPVSDLTTVAAKASIPFVVLPLLTCAITFVT"
     gene            complement(467..1318)
                     /locus_tag="VM910_02995"
     CDS             complement(467..1318)
                     /locus_tag="VM910_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61553.1"
                     /translation="MRKAFGSTIAVDGIDLRVEEGRILGLIGPNGAGKTTALNAIVGL
                     TPYQGELRVLGRDPWRERDRLMRDVCFIADVAVLPRWLRVSQALDYVEGVHPRFDRAK
                     AEGFLAKTTIGRNSKVRELSKGMVVQLHLALVMAIDARLLVLDEPTLGLDILYRKQFY
                     DSLLTDYFDRSRTIIVTTHQVEEIQDVLTDLMFINRGRIVLNCSMEEFESRYLEVIVN
                     PENLAAARALKPMHERQVFGRSILLFDRVDRQQLATLGDVRTPSIADLFVAVMSSQAG
                     ARQGVAQ"
     gene            complement(1347..>1718)
                     /locus_tag="VM910_03000"
     CDS             complement(1347..>1718)
                     /locus_tag="VM910_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI61554.1"
                     /translation="DSQPIYRQLRDRVVAMILDGVLKEGDPLPSVRNVAADYRVNPLT
                     VLKGYQELVDERLVEKRRGLGMFIKEGARNLLLQGERQKFLSEEWPRIHATIQRLGLK
                     TEELLDGGAGRPPSDAAKEER"
BASE COUNT          346 a          560 c          488 g          324 t
ORIGIN      
        1 tgcgtgacaa acgtgatggc gcaggtgagc aacgggagca ccacaaacgg aatactcgct
       61 ttcgccgcca ccgtagtgag atcagagacc ggcagcgact tccagaacag gatgctgcga
      121 tcgcgccgct ctctctgcag cgcctcgatg cagtagaaca ccgagacgat caggaaagtc
      181 cccatcagca gcgccgccgc caggtcgtag tgcgaggcga ccgcatggcg ctggcgcgct
      241 gggtccagcg ccgataaggc acgcattctg tgcggcagac caatcgtact aatcaaggag
      301 ccgaacagga tgaccgccgc gacagcaagt ggtgcgacgt agatcgcgcg gttctcccac
      361 agttcgcggc gcaacgccca gtacgtgtgc ctgagcgccg ggatctcagc tggggcgatg
      421 gccagcgatc cgtccgaaga ttccggcaga gcatccagtt gcgtattcat tgcgccactc
      481 cttgccgcgc gcccgcttga ctgctcatca ccgcaacgaa caagtccgcg atgctcggcg
      541 tgcgcacatc gcccagtgtt gcgagttgct ggcgatcgac gcgatcgaac agcagaatgc
      601 tgcgcccgaa cacctggcgc tcgtgcatcg gcttgagcgc ccgtgccgcc gccagattct
      661 ccggattcac gatcacctcc agatagcgcg actcgaactc ttccatgctg caattcagaa
      721 cgatgcggcc gcggttaatg aacatgagat ccgtgaggac atcctggatc tcttccacct
      781 ggtgggtcgt cacgatgatc gtgcggctgc ggtcgaagta atcggtcaac agcgaatcgt
      841 agaactgctt gcgatacagg atgtccaggc caagcgtcgg ctcgtccagc accagcaatc
      901 tcgcgtcaat ggccatgacc agagcgaggt gtagttggac caccatgcct ttcgacaatt
      961 cccggacctt gctgtttcgc ccgatggtcg tcttcgcaag aaagccttcc gccttcgcgc
     1021 ggtcgaatcg cggatgcacg ccttcaacgt agtcgagcgc ctgcgaaacc cgcagccaac
     1081 gcggcagcac cgcgacatcc gcaataaagc agacatcacg catgagccgg tcgcgctccc
     1141 tccaggggtc gcgtccaagg accctcagtt ctccctgata cggagtgagg ccgacaatcg
     1201 cgttcagcgc cgtggtcttg cctgcgccgt tcgggccgat gagtcccagg atgcggccct
     1261 cttcgacacg caaatcgatg ccgtccaccg caatggttga gccgaaagcc ttgcgcaagc
     1321 cacaagcttc tatgcatgac atggcttcag cgttcctcct ttgcggcatc cgacggcggc
     1381 cggccggctc cgccgtccag cagttcttcc gtcttcaggc ctagccgctg aatcgttgcg
     1441 tggattcgag gccattcttc gctcagaaat ttctgccgtt cgccctgcaa caacagattg
     1501 cgcgcgcctt ccttgataaa cattccaaga ccgcgcctct tctcgacaag ccgctcatcc
     1561 accaattctt gatagccctt cagaaccgtg agcggattga cccggtaatc cgccgcaacg
     1621 tttcgcaccg atggcagcgg atcgccttcc ttgagcacgc cgtcgaggat catggcgacg
     1681 acgcggtcgc gaagctggcg gtaaatcggc tgactgtc
//
LOCUS       DATLJT010000138         2508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_466038,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000138 DATLJT010000000
VERSION     DATLJT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466038"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(559..1137)
                     /locus_tag="VM910_03005"
     CDS             complement(559..1137)
                     /locus_tag="VM910_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61555.1"
                     /translation="MNPYLAKLRLWDETHHPYGPSKPSKPILSVVDHANTTGGTGFEG
                     FEGDLSRCLPENEAAADRGRSDTFGGLGRTYAALAARSPEHVPTERWQQAVQDGRRFL
                     AKWGEQAAALGWTPRDLFGLQKSPDQPHPSYRRLSRYDETGLIWLLQGRPVVALTDST
                     AAIENPTGAITIYRRYDKPALGPLGDSLDDFQ"
     gene            complement(1134..>2508)
                     /locus_tag="VM910_03010"
     CDS             complement(1134..>2508)
                     /locus_tag="VM910_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HVI61556.1"
                     /translation="GRRIAVKPQARTDWIESPNLWGCFIGRPGMLKSPAMMEALKPLH
                     HLEAEAAKAHTEALVDYRAALAEFKLHKSVKESILKEELKKKVAEKVVSLGGVSSLKD
                     MIKDAQKEDPLGLGAGPEEPTPVRYRTNDSSYEALGALLVSNPAGLLVERDELVSLLK
                     HLDREEQSVARGFYMTGWSGMHPYTFDRITRGHIHVEAVCIGVLGNTQPARIAEYIHR
                     ANYGGAGGDGLIQRFNLLVWPDAPADWKDVDEYPNREAREKAWEVYQRLSKIDESAAL
                     KLGAEKGPYDKVPALRFDEAAQGDFRNWRTDLERRLHSGDLSPALEGHLAKYRKLVPT
                     LALMNHLADRGEGLISQRSLLKALATAYYLESHARRVYGSGHEAELAAAKAILKHIRN
                     GDLTEGFTARDVHQRGWAHLTEREHVGWGLSLLVDLDYLAPSALSAGVEGGRPKVTYL
                     VNPRSLT"
BASE COUNT          448 a          772 c          767 g          521 t
ORIGIN      
        1 cgcgtgctgc gcggcatgcg agctcgcgat tgcgcccgtc gagaacctgg ccctcgtaga
       61 gcgtgatggg attgagcagg ccctgctcgg cgatgtcgtc aactaggcgg tcgaactcaa
      121 ctcccccgat gagcggcagc aggtcggcaa gcgggtgaaa tgctttcgcg gtcataactg
      181 ctgttgccct gtctctctgg cacggccgtg acgcgtcacc ctctcgtcgt tgcgcgtcac
      241 catgtgcctt gctgcgattg ggtttgcatc gagatatcga tggagcgcgg ccgctaaagc
      301 gctacgatcc ttccgctcgg cgggttccaa caatccacgt gcgatgaggg cgtccacttc
      361 agttcgccgc aactggacct tgatgcacaa aaggccacgc ctgcggcgct cacggtagcg
      421 gcgcatccgt tcggcggcag gggtcgggct cgcatcaatc atcgtgtccg ttcgtcttgc
      481 aggaggccgc ggcgcggcaa tgcagagaat atctccgtga cgacttgcct cgacggttcg
      541 gaaggcgttt gcgggcgatc attgaaagtc gtccaggctg tcgccgagcg ggccaagtgc
      601 cggtttgtca tatctgcgat agatggtgat ggccccggtg gggttctcga tcgcggcggt
      661 ggagtctgtg agcgccacaa ccggccgccc ctgcaggagc cagatgaggc cggtctcgtc
      721 ataccgggat aggcgacgat aagacgggtg cggctggtcg ggcgacttct ggaggccaaa
      781 caggtctcgg ggcgtccagc ctagcgccgc ggcctgctcg ccccatttgg caaggaagcg
      841 gcgaccatcc tgtacggcct gctgccagcg ctcggtggga acgtgttcgg gagagcgcgc
      901 cgccaaagcg gcgtaggtgc gaccaagacc accaaaggtg tctgatcgac ctcggtctgc
      961 ggccgcttca ttctcgggaa ggcacctact taagtcaccc tcaaaacctt cgaaacctgt
     1021 cccaccggtt gtgttcgcat ggtccacgac cgataagata ggttttgagg gttttgaggg
     1081 tccgtaaggg tggtgtgttt cgtcccacaa tcgcagcttc gctaagtacg ggttcatgtc
     1141 aggctccttg gattgaccaa gtacgtgacc ttgggccggc cgccctccac gccggcgcta
     1201 agggcagacg gggcgagata gtcgaggtcg acaagtaggc tcagccccca gcccacatgc
     1261 tctcgctcag ttagatgcgc ccagcccctc tgatggacat cgcgggcggt gaagccctca
     1321 gtgagatccc cgtttcggat gtgcttcagg atcgccttgg cggccgccaa ctcggcctca
     1381 tggcccgacc cgtagacgcg ccgggcatgg ctctccaagt agtacgcggt agccagtgcc
     1441 ttgagcaggg agcgctggga aatcagtccc tccccgcgat cggcaagatg attcatcagc
     1501 gccaaggtcg ggacgagctt gcgatatttc gcgagatgcc cctctaaggc gggcgagagg
     1561 tcgccggaat gcaaccgcct ttccaagtct gtccgccagt tgcggaagtc cccttgcgct
     1621 gcttcgtcga agcgcaacgc cggcaccttg tcgtaaggtc ctttctcggc gccgagcttt
     1681 agcgctgcgc tttcgtcaat cttgctgagg cgctgataaa cctcccaggc tttttcgcgc
     1741 gcctcacgat tcggatactc gtcgacatct ttccaatcgg caggcgcatc gggccagacc
     1801 agaagattaa agcgttgaat gagcccatcg ccgccggcgc cgccataatt cgcgcgatga
     1861 atgtactccg caatccgggc tggttgggta ttgcccagca ccccgatgca gaccgcctcg
     1921 acatgaatgt gaccgcgagt gatgcgatcg aaggtatagg ggtgcatgcc cgaccagccg
     1981 gtcatgtaga atcctctcgc gacagattgc tcctcgcggt cgaggtgctt gagcagggaa
     2041 acgagttcgt cgcgttcgac caggagcccg gccgggtttg ataccagcaa cgcaccaagc
     2101 gcctcgtagc tcgaatcgtt ggtgcgatag cggaccggcg tcggttcttc cggcccggcg
     2161 ccaagtccta gcggatcttc cttttgcgca tccttaatca tgtccttgag cgaggagacg
     2221 ccgccgaggc taacgacctt ctcggcaacc ttctttttca gctcctcttt cagaatgctc
     2281 tccttcaccg atttgtgcag cttgaactcg gcgagcgctg cccggtagtc taccaatgct
     2341 tccgtatgtg ccttcgcggc ctctgcctcg agatggtgaa gcggcttaag cgcctccatc
     2401 atcgccggcg acttcagcat cccgggtcgc ccgatgaagc agccccataa atttggactt
     2461 tcaatccaat cggttcgtgc ttgcggcttg accgcaatac gacgacca
//
LOCUS       DATLJT010000139         1607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2304295,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000139 DATLJT010000000
VERSION     DATLJT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304295"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VM910_03015"
     CDS             complement(<1..134)
                     /locus_tag="VM910_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI61557.1"
                     /translation="MPEFSTTRRVRHSAAHMFDLVADVERYPEFVPLCRSLKVRKRIP
                     "
     gene            complement(134..676)
                     /locus_tag="VM910_03020"
     CDS             complement(134..676)
                     /locus_tag="VM910_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HVI61558.1"
                     /translation="MQSSRAPDGSIHRVMVVGCAGAGKTTLARSLAARLGLPLVHLDF
                     HFWRAGWQLPDSKDWREQVIALAAAPEWIMDGNYSNTYDVRMPRADTLVWLDYARSVC
                     ARRVLLRTLTGYGRTRPDLPQSCPERFDLPFLRYVWNFPEKHRPRIVNAIECFGGHLR
                     IARLRSDRDAREFLAMLGRS"
     gene            complement(654..>1607)
                     /gene="lipA"
                     /locus_tag="VM910_03025"
     CDS             complement(654..>1607)
                     /gene="lipA"
                     /locus_tag="VM910_03025"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVI61559.1"
                     /translation="MTVNLDASSSPRHPEKAHRPDAPALKKPDWIRVKAPVSKGYGET
                     LAIVRANGLHTVCEEAGCPNIGECWDKKHATFMLMGDTCTRACAFCNVKTGLPGPLDA
                     AEPGRVAEATAKLGLRHVVVTSVDRDDLDDGGAAHFAAVIRAIRARCPVTTIEVLTPD
                     FLRKEGAVETVVAAGPDVFNHNLETVPSKYLTVRPGARYFASIRLLQRVKEVEPTMFS
                     KSGIMVGLGEERNEILQVMDDLRAADVDFLTIGQYLQPTRKHHPVVRFVPPDEFKAYE
                     TIAYAKGFLMVSSSPLTRSSHHAGENFARLKAARDAKLARP"
BASE COUNT          297 a          501 c          531 g          278 t
ORIGIN      
        1 tcggggatgc gcttgcggac cttgagcgag cggcacagtg gcacgaattc cggatagcgc
       61 tccacgtcgg ccacgagatc gaacatgtga gcggcggagt gccgcacccg ccgtgtggta
      121 gagaattcgg gcatcaactc cgtccgagca ttgcaaggaa ttcgcgggca tcgcgatcgc
      181 tgcgaagccg ggcgatgcgc agatgaccgc cgaaacattc gatcgcattc acgattcgcg
      241 gccggtgctt ttccggaaaa ttccagacgt agcgaaggaa tggaaggtcg aaccgttccg
      301 gacacgattg cggcaagtcc ggccgcgttc ggccgtagcc cgtcagcgtg cgcagcagca
      361 cgcgccgcgc gcagaccgag cgggcataat cgagccagac gagagtatcg gcacgcggca
      421 ttcgaacatc gtaggtattg gaataattcc cgtccatgat ccactcgggt gctgccgcaa
      481 gcgcgatgac ttgttcccgc caatctttgc tatcgggcaa ctgccaacct gcacgccaaa
      541 aatgaaaatc gagatggacc aacggcagcc ccaaccgtgc ggcgaggctg cgcgccaacg
      601 tcgtcttgcc cgcgccggcg caaccgacga ccatcacgcg gtgtatcgat ccgtcagggg
      661 cgcgcgagct ttgcatcgcg ggccgccttg agccgcgcga aattctcgcc cgcatggtgc
      721 gaggagcgcg tgagcggcga cgaggaaacc atcaagaacc ccttggcata ggcgatggtc
      781 tcgtaggcct tgaactcgtc gggcggcacg aagcgcacga ccggatggtg cttgcgcgtc
      841 ggttgcaggt actggccgat ggtgaggaag tcgacgtcgg ccgcgcgcag gtcgtccatg
      901 acctggagaa tttcgtttcg ctcctcgccg aggccgacca tgatgccgga cttactgaac
      961 atcgtgggtt cgacctcctt gacccgctgc agcaggcgga tcgaggcgaa gtagcgcgcg
     1021 ccgggtcgca cggtgagata cttcgaaggc acggtctcga gattgtggtt gaagacgtcg
     1081 ggccctgccg cgaccaccgt ctcgaccgcg ccctccttgc gcagaaaatc gggcgtgagc
     1141 acttcgatcg tcgtgaccgg gcagcgcgcg cggatggcgc gaatgacggc ggcaaagtgg
     1201 gcggcgccgc cgtcgtcgag atcgtcgcga tcgaccgagg tcacgaccac gtgcctgagg
     1261 ccgagctttg ccgtcgcttc ggcgacccgt ccgggctcgg cggcatcaag cgggccgggc
     1321 aagccggttt tgacgttgca gaaggcgcaa gcgcgcgtgc aggtgtcgcc catcagcatg
     1381 aaggtcgcgt gcttcttgtc ccagcactcg ccgatattgg gacaccccgc ctcctcgcag
     1441 acggtatgca agccgttcgc gcgcacgatc gcgagcgtct cgccataacc tttcgacacc
     1501 ggcgccttga ccctgatcca atcgggcttt ttcaacgcag gtgcgtccgg ccgatgcgcc
     1561 ttctcgggat ggcgcgggct gctgctggcg tcgagattga cggtcat
//
LOCUS       DATLJT010000140         3071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3223294,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000140 DATLJT010000000
VERSION     DATLJT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223294"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VM910_03030"
     CDS             complement(<1..743)
                     /locus_tag="VM910_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVI61560.1"
                     /translation="MRLRDAVFPVLSSFIFLTLLSASPGRAAGAVVRDSHTIQLGEVT
                     YRLDGVDAPEFDQMCIDDHADPWSCGVDARDQLTRLIGGRPVHCDDLGPDKNFGKWHI
                     GLCTVEGETVSLNQQLVQQGFAMDFEPSAKGRFLDDEARARDAQLGLWKGCFVAPDDF
                     RLGKKDGALRGSACRTDRDRQIREALFPEDLAMPPACSIKGKFAARARVTGDVGIYHL
                     RGCSSYAGLTQPDRWFCTEEDAQAAGFRRA"
     gene            complement(803..2467)
                     /locus_tag="VM910_03035"
     CDS             complement(803..2467)
                     /locus_tag="VM910_03035"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI61561.1"
                     /translation="MADSRPEEPSPEPSPNAASDAAADLDFDVIIIGAGMSGLYQLYR
                     MRELGLRVRVFETGTNIGGTWYWNRYPGARFDSESYSYGYSFSKELLEEWEWSEHFAG
                     QPETLRYLNHVADKFDLRRDIQFCSRVTAAVFDEEARSWTVTLEDESTYHTRFLITAI
                     GPLSTPTLPRIEGRDSFQGQSFHTARWPKTPVALAGKRVAVIGTGATGVQTIQTIAAE
                     VGHLTVFQRTPNWCAPLHNGKIDAETQRKIKAGYPEMFARCQETFACFIHTPDPRGAF
                     EVSDEEREAFYEKLYAERGFGIWQGNFKDILTDRRANATISDFVARKIRERVKDQKVA
                     DKLIPRNHGFGTRRLPLETFYYEVYNRDNVELVDLTETPIERITPKGIKTSEREYPFD
                     IIIFATGFDAITGAFDKIELRGTGGARLKDKWRDGPETYLGLMVEGFPNMLMLMGPHT
                     ALGNIPRSIEYSVEWVTGLIRFAQEKGLTRLEATAQGVASWTDHVKALGVGLLSNEVD
                     SWMTGINRNVDGKTKRIVARYSGSAPAYRERCDAVAAKDYDELRLA"
     gene            2674..>3071
                     /locus_tag="VM910_03040"
     CDS             2674..>3071
                     /locus_tag="VM910_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVI61562.1"
                     /translation="MNDPVDVLIIGAGASGAAVAWSLAETKMHILCLEQGGWMNPAEY
                     PSTGRDWEARFYGDWSTSPNVRARPEDYPINDDNSPIKVVNFNGVGGSTIMYTAHWPR
                     LHPSDFRVKSLDGVADDWPIDYDMLVPFFE"
BASE COUNT          539 a          968 c          963 g          601 t
ORIGIN      
        1 gcccggcgaa agcccgcagc ctgcgcatcc tcctcggtgc agaaccagcg gtcgggttgg
       61 gtcaggccgg catagctgct gcagccccgc aggtgataga tgccgacatc gccggtcacg
      121 cgcgcgcgcg cggcaaactt gcccttgatg ctgcaggccg gcggcatcgc gagatcctcc
      181 gggaaaagcg cctcgcggat ctgccggtcg cgatcggtcc ggcatgcact gccgcgcagg
      241 gcgccgtctt tcttgccgag gcggaagtcg tccggcgcga caaagcagcc tttccagaga
      301 ccgagctgag catccctggc gcgcgcttcg tcgtccagga agcggccttt tgccgagggc
      361 tcgaaatcca tcgcaaagcc ctgctggaca agctgctggt tcaagctcac cgtctcgcct
      421 tcgaccgtgc aaaggccgat gtgccatttg ccgaaattct tgtcgggtcc gagatcatcg
      481 cagtgaaccg ggcgtccgcc gatcagcctg gtgagctgat cgcgcgcatc gaccccgcag
      541 ctccaggggt cggcgtgatc gtcgatgcac atctggtcga actcgggcgc atcgacgccg
      601 tcgagccggt aggtcacctc gccaagctga atggtatggc tgtcgcggac cacggcgcct
      661 gctgcccgtc cgggacttgc ggataaaagc gtgagaaaga tgaaggacga caaaaccgga
      721 aaaacagcgt cacgtaaccg catgcctgct cgcaagatcg gaagctttat ggaagtctcc
      781 gtagcatatc ccgatgtcgg cgctacgcca gcctgagctc gtcataatct tttgccgcaa
      841 cggcgtcgca cctttcgcga taggccggcg cgctgccgct ataacgcgcg acaatccgct
      901 tggtcttgcc gtcgacgttc cggttgatcc cggtcatcca ggaatcgacc tcgtttgaca
      961 gaagaccgac gccgagcgcc ttgacgtggt cggtccatga cgcgacgcct tgggccgtcg
     1021 cctccagacg cgtcaggccc ttctcttgcg caaagcggat caggccggtg acccactcga
     1081 cgctgtattc gatgctgcgc ggaatgttgc cgagcgcggt gtgggggccc atcagcatca
     1141 gcatattggg gaagccctcg accatcagcc cgagataggt ctccggtccg tctctccatt
     1201 tgtccttcag ccgcgcgccg ccggttccgc gcaactcgat cttgtcgaac gcgccggtga
     1261 tcgcatcgaa gccggttgcg aaaatgatga tgtcgaacgg gtattccctt tcgctcgtct
     1321 tgattccctt gggcgtgatc cgctcgattg gcgtctccgt gaggtcgacg agctcgacat
     1381 tgtcgcggtt atagacctcg taatagaagg tctcgagcgg cagccgccgt gtgccgaagc
     1441 cgtgattcct ggggatcagc ttgtcggcga ccttctgatc cttcacgcgt tcgcggatct
     1501 tgcgtgcaac gaaatcgctg atcgtcgcat tcgccctgcg gtcggtcagg atgtccttga
     1561 agttgccctg ccagatgccg aagccgcgct cggcatagag cttctcgtag aatgcctcac
     1621 gctcctcgtc ggagacctcg aaggcgcccc gcgggtcggg cgtgtgaatg aagcaggcaa
     1681 aggtttcctg gcaacgggcg aacatctcgg gatagcccgc cttgatcttt ctttgcgtct
     1741 ctgcatcgat cttgccgtta tgcagcggcg cgcaccagtt cggcgtgcgc tggaacaccg
     1801 tgagatgacc gacttcggcg gcgatggtct ggatggtctg gacgccggtt gcgccggtgc
     1861 cgatgacggc aacgcgcttc cccgcaagcg cgacgggggt cttcggccag cgcgcggtgt
     1921 ggaatgactg gccctgaaag ctgtcgcgcc cttcgatgcg gggcagcgtc ggggtcgaga
     1981 gcggaccgat cgcggtgatc agaaagcgcg tgtgataagt actttcgtcc tcgagcgtga
     2041 ctgtccagct tcgtgcttcc tcgtcaaaga cggcggcggt gacccggctg cagaactgga
     2101 tatcgcgcct caagtcgaac ttgtcggcga catggttgag ataacggagc gtctccggct
     2161 ggccggcgaa atgctccgac cattcccact cctccagcag ctccttggaa aatgaataac
     2221 cgtaggaata gctttcggag tcgaagcgcg caccgggata gcggttccag taccaggtcc
     2281 cgccgatatt cgtgccggtc tcgaataccc gcaccctcag tcccagctcg cgcatccggt
     2341 agagctggta gaggccggac atgccggcac cgatgatgat gacatcgaag tccaggtcgg
     2401 ccgccgcatc ggatgcggcg ttgggcgatg gttctggcga tggttcttct gggcgcgaat
     2461 cggccaccgg cggctattcc cctgacattt ctgaattggc cgaaactatc ccgtctgccg
     2521 cctccggaca aatccgatag ctatgcgtgg gagggttgcg agcatgctcg cccgcgagcg
     2581 gcgcgccctt tgactgtagc cccgatctgc ccctatcgtt cggcccgccg atcgcccgcg
     2641 aagcggtgac ggcgcgtggg gaaaatgccc ggaatgaacg atcccgtaga cgtcctgatc
     2701 atcggcgccg gtgcgtcagg tgccgcggtc gcctggtcgc tcgccgagac caaaatgcac
     2761 atcctttgcc tggagcaggg cggctggatg aacccggccg aatacccgag caccggccgc
     2821 gactgggaag cgcgcttcta tggcgactgg tcgacgagcc cgaatgtccg cgcgcggccc
     2881 gaggactatc cgatcaacga cgacaattcg ccgatcaagg tcgtcaactt caacggcgtc
     2941 ggcggttcga ccattatgta cacggcgcat tggccgcggc ttcatccgtc cgacttccgc
     3001 gtcaagtcgc tcgacggcgt cgccgacgac tggccgatcg attacgacat gctggttccg
     3061 ttcttcgagg a
//
LOCUS       DATLJT010000141         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3682504,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000141 DATLJT010000000
VERSION     DATLJT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3682504"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VM910_03045"
     CDS             complement(<1..777)
                     /locus_tag="VM910_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61563.1"
                     /translation="MKRRDFIKVTGLGVAGATTIAAPAIAQSMPEIKWRMTTSWPKSL
                     DTLHGGAELMAKAVGEATDNKFQIQTFAAGEIVPGLQVLDAVQSATVEMGHTASYYYF
                     GKDPTFTFGSSVPFGPDMRLNQAWYLIGGGKEILNEFYKSYNVLSLLAGNTGCQMGGW
                     FRKEVNTVDDLQGLKFRIGGFAGKVLQKLGAVPQQLAGGDIYPALEKGTIDGAEWVGP
                     YDDEKLGFYKVAPHYYYPGWWEGGPMLLAMINLDKWNALPK"
     gene            complement(943..2031)
                     /locus_tag="VM910_03050"
     CDS             complement(943..2031)
                     /locus_tag="VM910_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI61564.1"
                     /translation="MKRRDFLKVSATGAAMAAVASPAIAQSSPEIKWRMTSSFPKSLD
                     TIYGASEVFAKYVAEMTDNKFQIQVFAAGEIVPGLQALDATSNGTVEMSHTVSYYYVG
                     KDPTFAIYASVPFGLNARQQNSWWYQGGGEELGNEFFKKFAVIGFPCGNTGTQMGGWF
                     RKEIKTVADLSGLKFRIGGIAGQVLQKVGVVPQQLAGGDIYPALEKGTIDGAEWVGPY
                     DDEKLGFAKVAKYYYYPGFWEGGPTVHAFCNLEKWNSLPKSYQTVVNAATTYANTWMA
                     ARYDLQNPSALKRLVAGGTQLRPFTNEVLEACLKATNELWAEISAKNPDFKKSIDAMQ
                     AYRSDEYLWWQVAEYTYDSFMIRSRTRG"
     gene            complement(2231..>2536)
                     /locus_tag="VM910_03055"
     CDS             complement(2231..>2536)
                     /locus_tag="VM910_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVI61565.1"
                     /translation="GLAMFKKVNVPVLGIVENMSYFQCPHCGTRSDIFGHGGARHEAE
                     RLGVPFLGEIPLHMAIRVTSDAGNPVVVSEPDGPHANIFRAIGAKVRDQLQGVIAAA"
BASE COUNT          463 a          789 c          761 g          523 t
ORIGIN      
        1 cttcgggagc gcgttccact tgtccaggtt gatcatggca agcagcatcg ggccgccctc
       61 ccaccagccg ggatagtagt agtgcggcgc gaccttgtag aaaccgagct tctcgtcgtc
      121 ataggggccg acccactcgg caccgtcgat cgtacccttt tccagagccg gatagatgtc
      181 gccaccggcg agttgctgcg gcacggcgcc gagcttctgc aacacctttc cggcgaagcc
      241 gccgatgcgg aatttcagac cctgcagatc gtcgacggtg ttgacctcct tgcggaacca
      301 gccgcccatc tggcagccgg tgttgccggc aagcagcgag aggacgttgt agcttttgta
      361 gaactcgttg aggatttcct tgccgccacc gatcaggtac caggcctggt tgaggcgcat
      421 gtccgggccg aacggcacgg atgagccgaa ggtgaaggtg gggtcctttc cgaagtaata
      481 gtacgaggcg gtgtgaccca tctcgactgt ggcgctctgc acggcgtcga gcacctgcag
      541 gcccggcacg atctcgcctg cggcaaaggt ctggatctgg aacttgttgt cggtggcctc
      601 gccgactgcc ttggccatca gctccgcgcc gccatgcagc gtgtcgagcg atttcggcca
      661 gctcgtcgtc attcgccatt tgatctcggg catcgactgc gcgatcgcgg gtgcggcgat
      721 cgttgtcgcg cccgcgacac ccaatcccgt gaccttgatg aaatcccttc gcttcatgtt
      781 tttccctctt tgaaagattc tgtgtcggcc ttcgtcttga ggcttggcct caagcatgga
      841 acatccggtc tccgatttcc atcccgctgc gacgaaaaag cccggcctcg tcaagaggcc
      901 gggcgtttta tctacgactt aaggcttagc gctttggttt aatcagccgc gcgtgcgcga
      961 gcggatcatg aagctgtcgt aggtgtattc ggcgacctgc caccacagat actcgtccga
     1021 acggtaggcc tgcatggcgt cgatcgactt cttgaagtcg ggattcttgg ccgagatttc
     1081 agcccacaat tcgttggtcg ccttaaggca ggcttccagc acttcgttgg tgaaggggcg
     1141 aagctgcgtg ccgccggcaa ccagccgctt cagcgcggac ggattctgca gatcgtaacg
     1201 cgctgccatc caggtgttgg cataggtggt cgcggcgttc acgacggtct gatagctctt
     1261 cggcagcgaa ttccacttct ccagattgca gaaggcgtgg acggtcggac cgccttccca
     1321 gaagcccgga tagtagtagt actttgcgac cttggcgaag ccgagcttct cgtcgtcata
     1381 gggtccgacc cattcggcgc cgtcgatcgt gcccttttcc agcgccggat agatgtcgcc
     1441 gccggcgagc tgctgcggca ccacgccgac cttctgcagc acctgccccg cgatgccgcc
     1501 gatacggaat ttgagtccgg aaagatcggc gacggtcttg atctctttcc tgaaccaacc
     1561 gcccatctgc gtgccggtgt tgccgcaggg aaatccgatc acggcgaact tcttgaagaa
     1621 ctcgttgccg agctcctcgc cgccgccctg gtaccaccag gagttctgct ggcgcgcgtt
     1681 cagaccgaac ggcacggaag cgtagatcgc aaacgtcgga tcctttccga catagtaata
     1741 ggagacggtg tgactcatct cgaccgtgcc gttggacgtc gcatcgagcg cctgcaggcc
     1801 cggcacgatc tcaccggccg caaacacctg gatctgaaac ttgttgtcgg tcatctcggc
     1861 aacgtatttc gcgaacacct cgctggcgcc ataaatcgtg tcgagcgatt tcgggaagct
     1921 ggacgtcatc cgccacttga tctcgggtga ggactgcgca attgccggcg aggccacggc
     1981 ggccatcgcg gcaccggttg cggaaacctt caaaaaatca cgacgcttca tctaagacca
     2041 tctccctggt tggggcgttc cccgtaattg cggttggcct gctccggggc ggcgcgaatc
     2101 tgcggtcccc aaggcccttc ggccgcgcgt ttaacacgga aggcgacggg cgaaaacgcg
     2161 acaatcgcat gactactgcc cactttccgg cagcaccggg ggcgattaaa cgaaagttgc
     2221 atgtggcagc tcaggcggcc gcgatcacgc cctgaagctg atctcgcacc ttggcgccaa
     2281 tggcgcggaa gatgttcgcg tgcggcccgt cgggctcgct gaccactacg ggattgccgg
     2341 catccgaagt gacgcggatc gccatatgca aggggatttc gccgagaaac ggtaccccga
     2401 gccgctccgc ttcgtgccgc gcgccgccat gaccgaagat gtccgagcgc gtgccgcaat
     2461 gcgggcactg gaaatagctc atgttctcga caatgccgag cacgggcacg ttgaccttct
     2521 tgaacatcgc aagtcc
//
LOCUS       DATLJT010000142         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_695695,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000142 DATLJT010000000
VERSION     DATLJT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695695"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VM910_03060"
     CDS             <1..266
                     /locus_tag="VM910_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI61566.1"
                     /translation="ETWVGFRPGSRDDAPMLGSSGIDRLIMATGHHRNGILLTPVTAG
                     SITAYVLTGRLPECAQPFTPERFLNARVGAAVRSGLVTAPEAR"
     gene            263..469
                     /gene="thiS"
                     /locus_tag="VM910_03065"
     CDS             263..469
                     /gene="thiS"
                     /locus_tag="VM910_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVI61567.1"
                     /translation="MTRVRINGVEEELAVATVVELLVALGVDPAARFLAIAVNGIVVR
                     RSEWPSRSLSPGDDVEIVRPFSGG"
     gene            495..1265
                     /locus_tag="VM910_03070"
     CDS             495..1265
                     /locus_tag="VM910_03070"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVI61568.1"
                     /translation="MTDPLLIAGTALSSRIFVGTAGYPNQQMLLDCLEASGAEIVTVS
                     IRRISLEGYAESLVDLLGGRYRLLPNTAGCATVRDAVLTAELAREALNTEWLKLELIG
                     DRETLYPDVEQLVRAAEELVRSGFTVLPYCNEDPVTCQKLADVGCAAVMPLGSLIGSG
                     MGIANPAAIELICSRSPVPVVLDAGIGTASDAALAMELGCDAVLVNTAIAKSHDPLRM
                     AAAMRAGVEGGRLARLAGRIPKRRFAEPSSPQLGLVGT"
     gene            1320..2126
                     /locus_tag="VM910_03075"
     CDS             1320..2126
                     /locus_tag="VM910_03075"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVI61569.1"
                     /translation="MIFAVPLYDDNPTARDPIVTYLLIGLCVGAFLWQLGQNERAILY
                     GYGMIPAELFGLWHPPRAYQVVPAWTTLLTSMFLHGGWFHLIGNMLFLWIFGNNIEDV
                     LGRGRYLFFYLCCGVVAALSQAFSNYGAHVPMVGASGAIAGVLGAYLVLYPNANVHVF
                     VWIVIFFRIVNIPAWILLGLWFAVQLLSGLARSPGTPGVAFWAHVGGFVSGVILVTLL
                     RPRGLALLQPPRSRVFATVPPSAFSGRRTFHRGSVPSVGRPYRRKPGLWD"
BASE COUNT          335 a          677 c          713 g          485 t
ORIGIN      
        1 tcgaaacctg ggtcgggttc cgcccgggtt ctcgcgacga cgcgccgatg cttggctcga
       61 gcggtatcga tcggctcata atggcgaccg gacaccatcg caacgggatc ctgctgacac
      121 cggtgacggc cgggtcgatc accgcctatg ttttgacagg ccggttgccc gagtgtgcac
      181 agccgttcac accggaacgt ttcctaaatg ctcgggtcgg cgccgctgtg aggtcgggcc
      241 tggtgaccgc tccggaggcg cgatgacgcg ggtgcggatc aacggcgtag aggaggaact
      301 cgcggtcgcg acggtcgtcg aactcctcgt cgccctcggc gtcgacccag ccgcgcggtt
      361 tcttgctatc gctgtcaacg gtatagtcgt ccgcaggtcg gagtggccct ccagatccct
      421 ctcgccgggc gacgacgtcg agattgtgcg cccgttctcc ggcggctgat gcaaccgctt
      481 ttggacctta tccaatgacc gacccgctac tgatagccgg caccgcgctc tcatcgagga
      541 tcttcgtcgg aactgccggc tacccgaacc agcagatgct gctcgactgc ctcgaggcga
      601 gcggcgcaga gattgttacg gtctcgatcc ggcggatcag cttagaaggc tatgccgaaa
      661 gtctagtgga tctactcggt gggcggtatc ggctgctacc gaacacggct ggctgcgcca
      721 ccgtccgtga tgctgttcta accgccgagc tcgcccgcga agcgctcaat acggaatggc
      781 taaagcttga gctgattggc gaccgtgaaa cgctttatcc cgatgtggag cagctcgttc
      841 gtgcagccga agagttggtg cgcagcgggt ttaccgtgct cccctattgc aacgaggatc
      901 cggtcacttg ccaaaagctg gccgatgtag gctgcgccgc ggtgatgccg ttggggtcgc
      961 taatcggctc ggggatgggt attgcgaacc cggcggcgat cgagctgatc tgctcgcgca
     1021 gccccgtacc agtggtgctc gacgccggta tcgggacggc ctctgatgca gcgctagcga
     1081 tggagctcgg ctgcgacgcg gtgttggtca acactgcgat cgccaagtcg catgacccgc
     1141 ttcgaatggc tgccgcgatg cgcgccgggg tcgaaggggg gcgtctcgcg cggctcgccg
     1201 gacgcattcc gaagcgacgt ttcgccgagc cgtcgagccc gcagctcggc ctcgtcggta
     1261 cctgagagga cgctcggttt ccgcttcccg agcggcttgg cgtcgtcggg tcgctcgaca
     1321 tgatttttgc cgtcccgctc tatgacgaca atccgaccgc ccgcgatccg attgtcacct
     1381 atctgctgat cggtctgtgc gtgggggcgt ttctttggca actcgggcaa aacgagcgcg
     1441 cgatcctcta cggatacgga atgatcccgg ccgagctgtt cggcctgtgg catccgccgc
     1501 gcgcctacca ggtcgtgccg gcatggacga cgttgctgac cagcatgttc ctgcacggtg
     1561 gctggtttca cctgatcggc aacatgctgt tcctgtggat cttcggcaat aacatcgaag
     1621 acgtgcttgg gcgcgggcgc tatctctttt tctatctctg ctgcggcgtc gtggccgcgc
     1681 tgagccaggc cttttcgaac tacggcgcgc acgtgccaat ggtcggggcg agcggcgcca
     1741 tcgccggggt cctcggtgct tatctggtgc tttatcccaa cgccaacgtt cacgttttcg
     1801 tctggatcgt gattttcttt cgtattgtca acataccagc ctggatattg ctcggtttgt
     1861 ggttcgcggt gcagctttta agcgggctgg cgaggtcgcc cggaactccg ggtgtggcat
     1921 tctgggcgca tgttgggggt ttcgtcagcg gagtcattct ggtcacgttg ttgcgcccga
     1981 ggggcctagc gctgttgcag ccgcctcgca gtcgtgtgtt cgcgacggta ccccctagcg
     2041 cgttctccgg tcgccgaact tttcatcgtg gttcggttcc atcggttggc cgcccgtatc
     2101 ggcggaagcc ggggctctgg gactgagcca agctgcttct attgagctcg aacttggctc
     2161 agctccgccg gccgcgcgac cttctgcaac agaaagagcc atttttccac
//
LOCUS       DATLJT010000143         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2993601,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000143 DATLJT010000000
VERSION     DATLJT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993601"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VM910_03080"
     CDS             complement(<1..1094)
                     /locus_tag="VM910_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61570.1"
                     /translation="MFNELDRRTSLTGNQIKILTAAIIGDALEFFDYFLIGFVLAFLI
                     GPWKLTFGQSATVLMSSGIGAIIGAYAWGWLADRIGRRKVFIATVLNFSIATGLLYFT
                     PDNGWIYLTILRFFVGTGVGGLYCVDLPLVQEFMPSSRRGFIGGLVTCVIPLGVGIGA
                     VLGAFMGTDQWRLLFAIGVLPSLLVLLVRLWVPESPRWLCRQGRYDEARKSLAWALQV
                     EPSTLPMPTAADAGPVLTTNWLDLFKYPRSLLVSWLGNAGAQTGVYGITLWAPSLFVL
                     LLKVSPQQAAKMMILLTVFGFIGRVSFSCLSELIGRRKAGGLLGLGAGVLTMVAGYNY
                     DVMWAGWSAFWLILALTSFFADGGFAIVGP"
     gene            1317..1577
                     /locus_tag="VM910_03085"
     CDS             1317..1577
                     /locus_tag="VM910_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVI61571.1"
                     /translation="MEERRKYQRTEINEPAYVSSGGSVMSCMVRNISPEGAAIDVENP
                     AFVPSRFRLVMARDASVRECSIAWIQKNRIGLTFVVPPQATE"
     gene            complement(1598..1888)
                     /locus_tag="VM910_03090"
     CDS             complement(1598..1888)
                     /locus_tag="VM910_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVI61572.1"
                     /translation="MTAALLVTSVVAQARAESGLASYYPGVGRSGEMTCAHRTRSFGS
                     MVIVRYRDRSVRCRINDRGPFVKGRVIDMSLSAARALGMIEAGVVLVSVHDE"
     gene            complement(2066..2707)
                     /locus_tag="VM910_03095"
     CDS             complement(2066..2707)
                     /locus_tag="VM910_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI61573.1"
                     /translation="MSTFPRALLVALALFAGVAGPSLSTAFAENPAAALVAKPGALPD
                     MALGSPKALVTIVEYSSMTCPHCAAFSENVFPMLQSKYIDTGRVRFVSRQFPLDMLAV
                     GAAMLAHCIANGDSPKFFDATSMIFKRQHELTQRPLDTLESVGDHFGMSGEAVKACEQ
                     DAGLFEKIKADHDLAFEQLKVDATPTFFVNGEKLRGSMSFEELDKKLTTLLER"
     gene            complement(2778..2906)
                     /locus_tag="VM910_03100"
     CDS             complement(2778..2906)
                     /locus_tag="VM910_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61574.1"
                     /translation="MKTISAIAADRSFKAMFVLSCFGLAVSVCLIVLGMDLNVAWL"
     gene            complement(3069..3275)
                     /locus_tag="VM910_03105"
     CDS             complement(3069..3275)
                     /locus_tag="VM910_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61575.1"
                     /translation="MRKQDIGFDYHRYKQLLADAVDEKKRLELIKILIEERAMERLAA
                     QRASDRAAMTAMTIAKVLAAPRNN"
     gene            complement(3416..3664)
                     /locus_tag="VM910_03110"
     CDS             complement(3416..3664)
                     /locus_tag="VM910_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61576.1"
                     /translation="MKSFIRVALLALIWATPSLAQRLVDPSKVAPEYREAAEKRRTEQ
                     IRQRDCAKKADTEKVMPRERTAFLLHCLEAAEAVEDSR"
     gene            complement(3834..4037)
                     /locus_tag="VM910_03115"
     CDS             complement(3834..4037)
                     /locus_tag="VM910_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61577.1"
                     /translation="MGMEFDPLTALTATGVAGYVAIIADSVARNDFGLSNGQIRTIAL
                     IAAMLIAVGLATDFLGRRLGKAK"
     gene            complement(4232..4429)
                     /locus_tag="VM910_03120"
     CDS             complement(4232..4429)
                     /locus_tag="VM910_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HVI61578.1"
                     /translation="MSTPACSFCGKKQSEVMQLVAGPQAFICDECSMLAVEMISVEHP
                     NWRERLMRTLALLPSGSGETT"
BASE COUNT         1005 a         1344 c         1425 g          815 t
ORIGIN      
        1 tagggtccga cgatggcaaa gccgccatcg gcaaagaagg atgtaagcgc caggatcagc
       61 cagaaggcgg accagcctgc ccacatgacg tcgtaattgt agccggccac catggtgagc
      121 acgcctgcgc caagacccaa caggccgccg gctttgcggc ggccgatcaa ttcggaaagg
      181 caggagaagg acacgcgtcc gatgaagccg aacacggtga gcaggatcat catcttggcg
      241 gcctgctgcg gcgatacctt caggagcagg acgaacagcg agggcgccca cagcgtgatg
      301 ccgtagacgc cggtctgcgc accggcattg ccgagccagg agacgaggag gctgcgcggg
      361 tatttgaaga ggtcgagcca gttggtcgtg aggactggac cggcatcggc cgccgtcggc
      421 atcggcagcg tggagggctc cacctgcagc gcccaggcga gcgacttgcg ggcctcatcg
      481 tagcggccct ggcggcacag ccagcgcggg ctctcaggca cccagagccg gaccagcagc
      541 acgagcaacg acggcagcac gccgatggca aacagcaggc gccactggtc ggtgcccatg
      601 aacgcgccaa gcacggcgcc gatgccgacg ccgagaggaa tgacgcaggt gacgaggccg
      661 ccgatgaagc cgcgcctgga ggaaggcatg aactcctgca cgagcggcag gtcgacgcaa
      721 taaagaccgc cgacgccggt gccgacgaag aaacgcaaaa tggtgagata gatccagccg
      781 ttgtcgggcg tgaagtagag cagacccgtt gcaatcgaga agttgaggac ggtggcaatg
      841 aagaccttgc gacggccgat gcgatcggca agccagcccc atgcataagc gccgataatg
      901 gcgccgatgc ccgaactcat caacacggtt gccgattggc cgaaggtcag cttccaggga
      961 ccgatcagaa aggcgagcac gaagccgatc aggaagtagt cgaagaactc cagggcgtcg
     1021 ccgatgatgg cagcggtcag gatcttgatc tggttgccgg tcagactcgt gcgtcggtca
     1081 agttcattga acatggcgtc tcccacttgg cgcatgttca ttttttgcac agcgagcgtc
     1141 gcgccatcgc tccgcctgca agaaggctac cctgtgatga ccgcgaacgg caagacggtg
     1201 tcagaacact ccccccatcg cgcaaatgca tatgcgcggt gctccgccgg cgccgagcca
     1261 cctgatttgt ttaaggtttg ccgctagtgt cccgcccgag gggatgggga agggctatgg
     1321 aagaaaggcg aaagtatcaa aggaccgaga tcaacgagcc tgcctatgtg tcgtcaggag
     1381 gttcggtgat gagctgcatg gtgcgtaaca tctcgccgga aggcgcggcg atcgacgtgg
     1441 aaaacccggc cttcgtgccg tcacgctttc gcctggtgat ggcgagagac gcatctgtcc
     1501 gcgagtgcag cattgcctgg attcaaaaga accggatcgg gctgactttc gtagtgcccc
     1561 cgcaggcgac agagtgaatc gaaaacctcg gcttgcatta ttcgtcatgc accgagacaa
     1621 gaacgacgcc cgcttcgatc atgccgagtg cgcgtgccgc gctcagcgac atgtcgatga
     1681 cgcgtccttt gacgaaagga ccacgatcgt tgatgcggca gcgaaccgac cggtcgcgat
     1741 acctcacgat taccatgctg ccgaacgaac gcgtcctgtg cgcgcaagtc atctcgccgg
     1801 agcgaccgac gccgggatag taggaagcca agccgctctc cgcgcgcgcc tgggcgacaa
     1861 cacttgtgac aagcaacgcc gcagtcaggc cagtaagtcg ggtccgaaac aattgacacc
     1921 gtctgtgccc tacaccgcag cgataagccc gcggaacaag ctaagttccc gaactatttt
     1981 tcgatgattg cacctagaaa cggaggaact ttttggagca agccgaaatg tgaacgaggt
     2041 ccggagccga gaggaagcgc gcaggtcagc gttcgagaag cgtcgtcagc ttcttgtcca
     2101 gctcctcgaa cgacatcgag ccccggagct tttcaccatt gacgaagaag gtcggcgtcg
     2161 catcgacctt caattgttcg aacgcgaggt cgtggtcggc tttgatcttt tcgaaaaggc
     2221 ctgcgtcctg ctcgcaagct ttgacggctt caccgctcat accgaaatgg tcgccaaccg
     2281 actccaacgt gtcgagcgga cgctgcgtca gctcgtgctg tcgtttgaag atcatcgagg
     2341 tggcatcgaa gaacttcggt gaatcgccat tggcaatgca gtgcgccaac atcgctgcgc
     2401 ctacggccag catgtcgagc gggaactgtc gcgagacgaa acgtaccctg ccggtgtcga
     2461 tatatttcga ctgcagcatg ggaaagacgt tttcggagaa ggccgcgcaa tgcggacagg
     2521 tcatcgaaga atattcgacg atggtgacca gtgccttggg cgatcccagt gccatgtcgg
     2581 gaagggcgcc gggcttggcg accagcgcgg cggcggggtt ttctgcgaac gcagtcgaaa
     2641 gcgaagggcc ggcaacgccg gcgaacaggg caagcgcgac aagcagcgcg cgcggaaacg
     2701 tcgacaatgg aacatccctt gggtttcgag cactcacaag cgcgcaatcg ccaacgcgag
     2761 cgcccggacc gaagagatta aagccaggcg acgttcaggt ccatcccgag cacgatcagg
     2821 cagacggaga cggcaagacc gaagcaggac aacacgaaca tcgccttgaa cgatcggtcc
     2881 gcagcgatcg cggaaatcgt tttcatggta accccttttg cttcgcccaa gcgggcgtca
     2941 gagggagcgt ttttaagaca aaagtcttta cgcgcgattg cccgaaatcg ttaatcaatt
     3001 gcaatgagac gcgcagaaac tgcggccgtt tgaggaccta tctccgagag ggccgagaaa
     3061 ttgcgaagtc agttgttgcg cggtgcagcc aacaccttgg cgatggtcat ggcggtcatc
     3121 gccgcgcggt cagatgcacg ctgcgccgcg agccgctcca ttgcccgttc ctcgatcagg
     3181 attttgatca gctcgagccg ctttttctcg tcgaccgcgt ccgccagcaa ctgtttgtag
     3241 cgatgataat cgaatccaat atcctgctta cgcatgccat gccccccgca tacgctcgtc
     3301 cggaggcaaa gttttccgca gtggcgaagc gagcacaatg gtgtccgcta acgtatcaac
     3361 acctctctcc ggcgatcgac cgccgctgcg acagactggc agtcggggca atccgctatc
     3421 tggagtcctc caccgcctct gcggcctcga gacagtgcaa cagaaaggcg gtgcgctcgc
     3481 gcggcatcac cttctcggta tcggcttttt tcgcgcagtc gcgctgcctg atttgttcgg
     3541 tacggcgctt ttctgcggcc tcccggtact cgggagccac cttcgacgga tccacaagtc
     3601 tttgggcgag gcttggggtc gcccatatga gcgcgagcag agccactcgg ataaaggact
     3661 tcaaaaaagc ctccattcaa aaatcgcgct tgagaaaaga aagaaatacg ccggtaacat
     3721 cctgctcttg cggccatcag aacgacaaac cggatcgacg tccagcaatt ttcgctgcaa
     3781 tcatgtgccg ctgtggcggg cgcccccggc ttctcgcaac acgcaccctg atctcatttc
     3841 gctttgccca atcgccggcc caggaagtcc gtggccaagc cgacggcaat cagcatcgcg
     3901 gcgatcaagg cgatggtgcg gatttggccg ttcgacaagc cgaaatcgtt gcgcgccaca
     3961 ctgtcggcga tgatcgcaac atagccggcg acgccggtgg cggtcagagc ggtaagtgga
     4021 tcaaattcca ttcccatcgt gcccgtcctt gcgaaagaca cgccatacta agcggcaagt
     4081 gggcttgccc gctgtgaggc ggttcacaat cagtgagacg gggtaggcgc cgatcgcgag
     4141 ggagtgcgaa caccaatgat ccgcgctgga gatcaagagg ccgccgtacc ttgcgcggcc
     4201 agcgcggcga aggtgatctt ggccgggcgg gtcacgtcgt ttcgccggaa ccactgggaa
     4261 gcaaagccag cgtcctcatg agacgttcgc gccagttggg gtgttcgacg ctgatcatct
     4321 cgacggccag catcgagcat tcgtcgcaga taaaggcctg cggcccggcg acgagttgca
     4381 tcacctcgct ttgcttcttg ccgcagaacg aacaggctgg cgtactcatt gtggccttcg
     4441 gggctctcct tgtctggcat tgcgatcacg gcgggcgagg gcggccatgg ccgatcatcc
     4501 cgccgatcgg gccgggaggg aagtcgccaa aagcctaaaa ctcgggtttt attgggtttt
     4561 ctttcatttt tggcggaagg ggtgggatt
//
LOCUS       DATLJT010000144         3430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_925928,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000144 DATLJT010000000
VERSION     DATLJT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925928"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..611)
                     /locus_tag="VM910_03125"
     CDS             complement(240..611)
                     /locus_tag="VM910_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61579.1"
                     /translation="MANEIVQSSRDWLSSPRTNALAWWIPKAVIVAGLFIPPPTRTGM
                     WIVALVWMGTACILNSRRCGRTHCRYTGPYYLAMIAPVLVFASGVIPVDFYGWLLLAI
                     LILGGSMIIWWATERAWGKFS"
     gene            complement(617..844)
                     /locus_tag="VM910_03130"
     CDS             complement(617..844)
                     /locus_tag="VM910_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HVI61580.1"
                     /translation="MIFESVITCPHCATAKSEAMPTDACRFFYTCTGCGATLRPKPGD
                     CCVFCSYGSVPCPPIQAERSGGTGAASCCGG"
     gene            complement(900..1115)
                     /gene="merF"
                     /locus_tag="VM910_03135"
     CDS             complement(900..1115)
                     /gene="merF"
                     /locus_tag="VM910_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system transport protein
                     MerF"
                     /protein_id="HVI61581.1"
                     /translation="MRDRALITTGIVGAAVAAVCCATPLLVVAFGAVGLTAWLAKADY
                     VLIPALILCLALVAFGLYRRRRRLHHG"
     gene            complement(1121..1405)
                     /gene="merP"
                     /locus_tag="VM910_03140"
     CDS             complement(1121..1405)
                     /gene="merP"
                     /locus_tag="VM910_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HVI61582.1"
                     /translation="MGKLLACVAMAVSVFASSAAFAAEKTITLAVKNMYCAACPHTVK
                     ASLQAVPGVKAVTVSFKDKTAVVTYDDTRTDVKALTAATTDAGYPSAPKS"
     gene            complement(1496..1858)
                     /locus_tag="VM910_03145"
     CDS             complement(1496..1858)
                     /locus_tag="VM910_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HVI61583.1"
                     /translation="MAGLRRGQGLMAAGGLLGALVASSCCLLPLVLFGLGVSGAWIGH
                     LTRLAPYQPFFIAATIACLGYGYWLVYRSSKVACAGGEACARPLSNRLVQLGLIVATV
                     LVIAAIGFDFLAPLLLDL"
     gene            1943..2365
                     /locus_tag="VM910_03150"
     CDS             1943..2365
                     /locus_tag="VM910_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI61584.1"
                     /translation="MTTLRADGVPVGELSRLTDVNIETIRYYEKIKMLPAPARTKGGH
                     RVYGPKETRILVFIRRGRELGFTLDQIRTLLNLAAPGKASCREVRAIAARHLEDVRAK
                     MADLEKLERLLAKTIKQCSGRAVPDCPLLDVLDASRTA"
     gene            complement(2494..>3430)
                     /locus_tag="VM910_03155"
     CDS             complement(2494..>3430)
                     /locus_tag="VM910_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVI61585.1"
                     /translation="KTGTLTEGKPKVVAIIAAKGFPENDALRWAASVEKASEHPLALA
                     IVAAAQERKLNLSGVAEFDSPTGKGVVGIVEGRRLALGNARFLRELGVQTNALEQDAE
                     RLRHEGATAIFLAVDGKVAAVIAIADPVKSTTPDALTALSREGIRVVMLTGDNKTTAD
                     AVARRLGITEVEAEVLPDQKSAVVTKLQKEGRAVAMAGDGVNDAPALAAAEVGIAMGT
                     GTDVAIESAGVTLLKGDLTGIVRARRLSQATMRNIRQNLFFAFIYNAAGVPIAAGVLY
                     PFFGILLSPIIAAAAMALSSVSVVGNSLRLRRVSL"
BASE COUNT          689 a         1096 c         1063 g          582 t
ORIGIN      
        1 ggcagctcga aggcaagatc cgctcgatgc gcgcgatgtg caaagcgctc gatcgcctca
       61 tcgccatctg tgtcgatgaa gcgcgccccg tcgaggcatg cgcgatcctt gccgccttcg
      121 agcaacccga gacaaccgca cggacgaact gaactgcgaa agaagagtcg gcatgatgat
      181 gcggctgaga caagtcggcg cgcgaacgaa ctccaagcgg taagtcacca gtcccttggc
      241 taggagaatt ttccccatgc cctttctgtg gcccaccaaa tgatcatgct tccgccaaga
      301 atgagaatcg ccaataaaag ccatccatag aagtcaacgg gaatgacacc cgaagcaaac
      361 acgagcaccg gtgcgatcat cgcgagataa taaggtcccg tgtaacggca atgggtccgt
      421 ccgcaccgtc ttgaattcag aatgcatgct gtacccatcc agacgagcgc gacgatccac
      481 atcccggtgc gcgtcggcgg gggaatgaaa agacccgcaa cgatcaccgc tttcggaatc
      541 caccatgcca acgcgttagt gcgagggctg ctgagccagt cgcgtgagct ttggacgatc
      601 tcgtttgcca tggcgcttat ccgccacagc aggaagcggc gcccgtccca cccgaccgct
      661 cggcctggat cggcgggcac ggaaccgagc cgtaggaaca gaacacgcaa caatcacctg
      721 gcttcggccg cagcgttgcg ccgcagccgg tgcacgtata gaaaaaccgg caggcatcgg
      781 tcggcatggc ctccgacttc gcggtagcgc aatgcggaca ggtgatgaca gattcaaaga
      841 tcatggttct tcctgaagca tgtagccgat atcggaggct ccggtcggat atccatccgt
      901 cacccgtggt gcaggcgtct gcgtctgcgg tagaggccga aggcaaccag cgcaagacac
      961 aggattagcg cggggatcag cacataatcg gccttggcaa gccaggcagt cagccccacc
     1021 gcgccgaacg cgacgacgag caacggggtg gcgcaacaga ccgccgcgac cgccgcaccg
     1081 acgatgccag ttgtaatgag ggcgcggtcg cgcatgccat tcagctcttt ggcgcggacg
     1141 gatagcccgc gtctgtggtc gcggccgtaa gcgccttcac gtcggtcctg gtgtcgtcgt
     1201 aggtgacgac cgccgtcttg tccttgaagg agacggtcac cgccttcacg ccgggcaccg
     1261 cttgcaggct cgccttaacg gtatgcggac aagcggcgca atacatgttc ttcacggcca
     1321 acgtgatggt cttctcagcc gcgaaagcgg cggatgaggc gaagacgctc acggccatgg
     1381 caacacaagc gagcagtttg cccatcggga ttctccgttc acgagttgag gaatagcggc
     1441 gctacgccga gccgcgcttt cagatgctcg attgctgtca cagggcatac tccgctcata
     1501 agtcgagcag caagggagcg aggaagtcga agccgatcgc tgcgatgacg agcaccgtcg
     1561 ccacaatgag cccgagctgc acgagccgat tcgataacgg ccgcgcacac gcttcaccac
     1621 cggcgcacgc aactttggat gagcggtaga cgagccagta cccgtaaccg aggcaagcga
     1681 tcgtcgccgc aatgaagaaa ggttgatacg gtgcaagtcg cgtgaggtga ccaatccacg
     1741 ccccgctcac gccgagcccg aacaggacaa gcggcaagag gcagcaggat gaggccacga
     1801 gcgccccgag aagaccgccc gctgccatca ggccttggcc gcgccgcaga ccggccatgc
     1861 gattttcttg attgatcgtc acggcccata agatgcatcc tgtagcgact acaggatcaa
     1921 ggggagattt tccgtgcgcc cgatcacaac tttgcgagcg gacggcgtgc cggtcggcga
     1981 gctttctcgc cttaccgacg tgaatatcga gacgatccgc tactacgaga aaatcaagat
     2041 gctacccgcc ccggcgcgca ccaagggcgg tcatcgggtc tatgggccga aagaaacgcg
     2101 catactcgtc tttatccgcc gcgggcggga gctggggttc acgctcgatc aaatccgcac
     2161 cttgctcaat ctggcagcac cgggaaaagc gtcatgccgc gaggtgcgtg cgatcgccgc
     2221 gcgccacctg gaggatgtgc gtgcaaaaat ggcggacctt gaaaaactcg aacgtctgtt
     2281 ggccaaaact atcaagcagt gttccggtag ggcggttcct gattgccccc tgcttgacgt
     2341 cctcgatgcg agccgtactg catagttctt gcgggcgggc ctcgacggca gctcctctgc
     2401 agtgcgttcc tcaacgaccg agatcggggc actgatcgtg cctctccgtc gcgaacagac
     2461 gtgtgcaggt tagcggatgg cgcgccgcgg agttcacaag gacacgcgtc gcaaccgcag
     2521 cgaatttcct accacgctga cggaggagag cgccatcgcc gccgcggcga tgatcggcga
     2581 gagcaggatg ccgaagaacg gatagagcac gccggcggcg atcggcaccc cggccgcatt
     2641 ataaatgaaa gcgaagaaca ggttctggcg gatgttgcgc atcgtcgcct gggagagccg
     2701 gcgcgcgcgc acgatcccgg tcaggtcgcc tttgagcaag gtgacgcccg cgctttcgat
     2761 ggcgacgtcg gtcccggtgc ccatggcgat gccgacttcg gccgcggcca gcgccggcgc
     2821 gtcgttgacg ccgtcgccgg ccatcgcgac cgcgcgtccc tccttttgca gcttcgtgac
     2881 gacggcgctc ttctggtccg gcagcacctc ggcttccacc tcggtgatgc cgagacgccg
     2941 cgcgaccgca tccgccgtgg tcttgttgtc gccggtgagc atcaccacgc ggatgccttc
     3001 tcgcgacagc gcggtgaggg cgtctggagt cgtgcttttc accggatcgg cgatcgcaat
     3061 caccgccgcg accttgccgt cgacggccag gaagatcgcc gtcgcgccct cgtgccgaag
     3121 ccgctcggcg tcttgctcca gcgcgttggt ttgaacgccg agctcgcgca ggaaccttgc
     3181 attcccgagc gcgagccgcc gcccctcgac aatgcccacc acgcccttgc cggtgggcga
     3241 atcgaattcg gcaacgcccg agaggttgag cttgcgctcc tgcgcggcgg cgacgatcgc
     3301 gagcgcgagc ggatgctcgc tcgccttttc cacgctcgcc gcccatcgca gcgcgtcgtt
     3361 ttcaggaaac cccttggccg cgatgatggc caccaccttc ggcttgcctt cggtgagcgt
     3421 gccggtcttg
//
LOCUS       DATLJT010000145         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_236424,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000145 DATLJT010000000
VERSION     DATLJT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236424"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /gene="petA"
                     /locus_tag="VM910_03160"
                     /gene_synonym="fbcF"
     CDS             <1..475
                     /gene="petA"
                     /locus_tag="VM910_03160"
                     /gene_synonym="fbcF"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HVI61586.1"
                     /translation="TGAAAAVGTMFTAWPFISQMNPDASTIAAGAPIEVDLSPIADGQ
                     DIKVFWRGKPIYIMHRTKKQIDEARAVPVDSLKDPATDQSRVKEGHDQWLVVIGICTH
                     LGCIPIAHEGNYDGFFCPCHGSQYDTSGRIRQGPAPANLTVPPYQFVSDTKIQIG"
     gene            544..2610
                     /locus_tag="VM910_03165"
     CDS             544..2610
                     /locus_tag="VM910_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HVI61587.1"
                     /translation="MSGPSDYKPNHPALQWVERRLPILGLMHSSFVAYPTPRNLNYWW
                     TFGAILSFMLGLQILTGVVLAMHYTPNADLAFRSVELIVRDVNYGWLLRNMHAVGASM
                     FFFAVYVHMFRGLYYGSYKEPREVLWILGVIIYLLMMATGFMGYVLPWGQMSFWGATV
                     ITNLFSAFPYVGDYIVTLLWGGYSVGNPTLNRFFSLHYLLPFVIAGVVVLHVWALHVA
                     GQNNPAGVEPKTEKDTVPFTPHATIKDLFGVSVFLLLYCWFIFYLPNYLGDADNYIPA
                     NPAQTPPHIVPEWYYLPFYAMLRSIPNKLAGVVVMFSSILILVFLPWLDTAKTRSSKY
                     RPLAKQFFWMFVVVGILLGYLGSKPPEGIYVIAGRILTACYFAYFLIVLPLLSRIEKP
                     RPLPNSIADDVLAKSGKPMPMVSTVIALMVAGGLLIGSAQNARAAEEDTPPANKWSFA
                     GPFGKYDRGALQRGLKVYKEVCSNCHGLSYVAFRNLAEPGGPGYTTAQAAALASEYKI
                     KDGPNDQGDMFERPGRPADYFPSPFPNEQAARASNGGAAPPDLSLITKARSYERGFPW
                     FIFDFFTQYQEQGPDYVSAILQGFEDKPPAGVTVPEGSYYNKYFPGHAIKMPKPLSDG
                     QVTFDDGAPATVAQYAKDVTTFLMWTAEPHMEARKQLGFQVFVFLIIFAGLMYFTKKK
                     VWADAH"
     gene            2782..3456
                     /locus_tag="VM910_03170"
     CDS             2782..3456
                     /locus_tag="VM910_03170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI61588.1"
                     /translation="MGTSITFKRPDGKEASGYLANAARGNAPGVVVIQEWWGLQDQIK
                     GMCDRFARAGFDALAPDLYKGKVVPYHDTDAAGKEMNSLDFMDATTQSVRGAVQYLAR
                     NGAKVGLTGFCMGGAVTIIGAAKIPELTAGVVFYGIPPEQAAKPADVRIPLQAHFANK
                     DDWCTPQAVDAWEKGMKAAGKALELFRYDAEHGFANEQRMTVHDRQAAELAWGRATEF
                     FRKHLG"
     gene            complement(3499..4491)
                     /locus_tag="VM910_03175"
     CDS             complement(3499..4491)
                     /locus_tag="VM910_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61589.1"
                     /translation="MLSTLGYAGFGSDRLEDWRQFGTGMVGFQAVERGNSLLAFRMDD
                     RKQRVVIDRAMGEGARFFGWEVADQTALDALAARLEKAGIRVTAEPQTRADARRVRAL
                     ISFHDPAGNRLEAFYGAEIDDTPFRPGRSISGFRTGALGLGHVVLTVENIEPVMAFYV
                     EVLGFRLSDYIEKPFRAYFFHLNPRHHSLALIETGRDGMHHLMVELFSLDDVGQAYDI
                     ALNEDRVNVTLGRHTNDFMTSFYARTPSSFMVECGWGGREIDPSTWRPVEMTHGPSLW
                     GHDRVWLSEKDREIAREMRMNAAREGLRAPVQVMEGNYTPMSGTCAWWDGVRGN"
BASE COUNT          856 a         1557 c         1424 g          927 t
ORIGIN      
        1 aactggagca gccgcagccg tcggcaccat gttcacggcg tggccgttca tatcccagat
       61 gaaccccgac gcctcgacga ttgcggcagg cgccccgatc gaggtcgatc tgtcgccgat
      121 cgctgacggg caggacatca aagtgttctg gcgcggcaag ccgatctaca tcatgcacag
      181 gaccaagaag cagatcgacg aggcgcgcgc cgtcccggtc gacagcctca aggatcccgc
      241 gaccgaccag tcccgcgtca aggaaggtca cgatcagtgg ctggtggtga tcggcatctg
      301 cacccacctc ggctgcatcc cgatcgcgca tgagggcaat tacgacggct tcttctgtcc
      361 ctgccacggt tcgcagtacg atacctccgg ccgcattcgc caggggcccg cgcccgccaa
      421 cctgacggtg ccgccgtatc aattcgtctc cgataccaaa atccagatcg gttgaggcgc
      481 gggacttccc gctccgggac ctcgaagcca atcgcgtcgc cttacttcct tcaggatcgc
      541 atcatgagcg gaccatccga ctacaagcca aaccatccag ccttgcaatg ggtcgagcgg
      601 cgcctgccaa ttcttggtct catgcactca tcgttcgtgg cctatccgac gccgcgcaac
      661 ctgaactact ggtggacctt cggcgccatt ctttccttca tgctcggctt gcaaatcctg
      721 accggcgtgg tcctggcaat gcactacacg ccgaacgccg atctcgcctt ccgttcggtg
      781 gaattgatcg tccgcgacgt caactacggc tggctcttgc gcaacatgca cgccgtcggc
      841 gcgtcgatgt tcttcttcgc cgtctatgtg cacatgttcc gaggcctcta ttacgggtcg
      901 tacaaggagc cgcgcgaggt gctgtggatc ctcggcgtca tcatctacct cctgatgatg
      961 gcgaccggct tcatgggcta cgtgctgccg tggggccaga tgagcttttg gggcgcgacc
     1021 gtcatcacca acctgttttc ggcgttccct tatgtcggcg actacatcgt cacgctgctc
     1081 tggggcggct actccgtcgg caatccgacg ctgaaccgct tcttttcgct gcactacctg
     1141 ctgcccttcg tgatcgcggg cgtggtcgtg ctgcacgtct gggcgctgca tgtcgcaggc
     1201 cagaacaatc cggccggcgt cgagccgaag acggaaaagg acacggtgcc gttcacgccg
     1261 catgcgacga tcaaggacct gttcggcgtc tcggtgttcc tgctgctcta ttgctggttc
     1321 atcttctacc tgccgaacta tctcggcgac gccgacaatt acattccggc caatccggcg
     1381 cagacgccgc cgcacatcgt gccggagtgg tactacctgc cgttctacgc gatgcttcgc
     1441 agcattccga acaagcttgc cggcgtcgtc gtaatgttct cctcgatcct gatactggtg
     1501 ttcctgccct ggctcgacac cgccaagaca aggtcgtcca aatatcgccc gctggcgaag
     1561 cagttcttct ggatgttcgt cgtcgtcggc atcctcctcg gctatctcgg ctccaagccg
     1621 ccggaaggca tttacgtgat cgccgggcgt attttgacgg cctgctactt cgcctatttc
     1681 ctgattgtcc tgccgctttt gtcccggatc gagaagccgc ggccgctgcc gaactcgatc
     1741 gccgacgacg tgctggcgaa gtcgggcaag ccgatgccga tggtttccac cgtgatcgcc
     1801 cttatggtcg caggcgggct tctgatcggc agcgcgcaaa atgcgcgcgc cgcggaagag
     1861 gatacgccgc cggccaacaa atggtcattc gcgggtccct tcggcaagta cgatcggggc
     1921 gcgttgcagc ggggcctgaa ggtctacaag gaagtctgtt ccaactgcca cgggctgtcc
     1981 tatgtcgcct tccgcaacct cgccgagccc ggcggccccg gctacacgac tgcgcaggcg
     2041 gcggcgctgg catccgagta caagatcaag gatggtccga acgatcaggg cgatatgttc
     2101 gagcggccgg gccggcccgc cgattatttc ccctcgccat tccccaacga gcaggccgcg
     2161 cgggcgtcca atggcggcgc agccccgccg gatctgtcgc tgattaccaa ggcgcgctcc
     2221 tatgaacgcg gcttcccctg gttcatcttc gacttcttca cccaatacca ggaacagggg
     2281 cccgactatg tctcggcgat cctgcagggt tttgaggaca agccgccggc cggcgtcacc
     2341 gtgccggagg ggtcgtatta caacaagtat ttccccggcc acgcgatcaa gatgccaaag
     2401 ccgctctccg acgggcaggt gaccttcgac gacggcgcgc ccgcaaccgt ggcgcaatac
     2461 gccaaggacg tcaccacgtt tttgatgtgg accgccgagc cgcacatgga agcgcgcaag
     2521 cagctcggct ttcaggtgtt cgtgttcctg atcatctttg ccggcctgat gtatttcacc
     2581 aagaagaagg tctgggcgga cgcgcactga cttttgtgaa ggcaatgtga atgggaaaaa
     2641 gcccccgcaa gggggttttt gtttgaacgc gtcatttcca ggttcgctcg cgcgccggaa
     2701 tgatggtaac cggattgcgt cgtgcgccgg caatcgccaa aataggttca gcgactactc
     2761 gaaaaaatcc tcggaggaac catgggcacc agcattacct tcaagcgccc ggacggcaag
     2821 gaagcgtccg gctatctcgc caacgcggca cgcggcaacg cgccgggcgt cgtggtgatc
     2881 caggaatggt ggggcctgca ggatcagatc aagggcatgt gcgaccgctt cgcgcgcgcc
     2941 ggcttcgacg cgctggcgcc cgatctctac aaaggcaagg tcgtgcccta tcacgacacg
     3001 gacgccgccg gcaaagagat gaactcgctc gatttcatgg atgcgaccac gcaaagcgtg
     3061 cgcggcgccg tgcaatatct ggcgcgcaac ggtgccaagg tggggctgac cggcttttgc
     3121 atgggcggcg ccgtgaccat catcggtgcg gcgaaaattc ccgaactcac cgccggcgtc
     3181 gtattctacg gcatcccgcc ggaacaggcg gccaagcctg cggatgtgcg tattcccctg
     3241 caggcgcatt tcgccaacaa ggacgactgg tgcacgccgc aagcggtcga tgcctgggaa
     3301 aaggggatga aggcggccgg caaggcgctg gagcttttcc gctacgacgc cgaacacggt
     3361 ttcgccaatg agcagcgcat gacggtgcat gaccgccagg ccgccgagct cgcctggggc
     3421 cgcgcgaccg agtttttccg gaagcatctg ggctaggacg caactccggg cagacgcata
     3481 ccccgcgcaa gcggggtatc aattcccccg caccccgtcc caccaggcgc aggtgccgga
     3541 catcggcgtg taattgcctt ccatcacctg caccggcgcg cgcaggcctt cgcgtgcggc
     3601 gttcatccgc atctcgcgcg cgatttcgcg gtccttctcc gacagccaga cgcggtcatg
     3661 cccccatagg ctcgggccat gcgtcatctc gaccggccgc caggtcgaag gatcgatctc
     3721 gcgtccgccc catccgcact ccaccatgaa cgacgacggc gttcttgcgt agaacgacgt
     3781 catgaaatcg ttggtgtgac ggccgagcgt gacattgacg cgatcttcgt tgagcgcgat
     3841 gtcgtaggct tggccgacat cgtcgagcga gaacaattcc accatcagat gatgcatgcc
     3901 gtctctgccg gtctcgatca gcgcgaggct gtgatggcgc ggattgaggt ggaagaaata
     3961 ggcgcgaaaa ggcttttcga tatagtcgga gaggcggaag ccgagcacct cgacatagaa
     4021 tgccatcacg ggttcgatgt tctcgaccgt cagcacgaca tggccgaggc cgagcgcacc
     4081 cgtgcggaat ccggaaatcg agcgtcccgg cctgaacggc gtgtcgtcga tctcggcgcc
     4141 gtaaaaggct tccagccggt tgcctgccgg gtcgtgaaac gagatcagcg cacgaacgcg
     4201 gcgcgcatcc gcccgcgtct gcggttcggc cgtaactctt atgcccgcct tttccaaccg
     4261 ggcggcgagc gcatccagcg cggtctggtc tgcaacctcc cagccgaaga agcgggcgcc
     4321 ttcgcccatg gcgcggtcga tcacgacgcg ctgcttgcgg tcgtccatcc ggaacgcaag
     4381 cagcgagttg ccgcgctcga cggcctgaaa tcccaccata ccggtgccga actgccgcca
     4441 atcctcgagc ctgtccgatc cgaagccggc atagccgaga gtgctgagca tggcaggcct
     4501 cctcttcaac gccggtctta acgtccggcc ctatcctggc cggaatccta aacctatttt
     4561 cgtggccgtc catggtgggg ctcccttgac gcggcagccc tgccatggtg tctacacagg
     4621 ccatgagcac tgccgtccgc ccctcccgtc gttggtggcc caccttctaa aaaggtggcc
     4681 ggtgcgattt tttcatcttt cgtcgtcgga ggccgccatc gcagtgcggc ggtccttcgt
     4741 ttgtcctgtg tggcgttccc tcgg
//
LOCUS       DATLJT010000146         4777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2534087,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000146 DATLJT010000000
VERSION     DATLJT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2534087"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..270
                     /locus_tag="VM910_03180"
                     /pseudo
     CDS             52..270
                     /locus_tag="VM910_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970894.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(320..393)
                     /locus_tag="VM910_03185"
     tRNA            complement(320..393)
                     /locus_tag="VM910_03185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(359..361),aa:Gly,seq:tcc)
     gene            complement(749..937)
                     /locus_tag="VM910_03190"
     CDS             complement(749..937)
                     /locus_tag="VM910_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61590.1"
                     /translation="MIVPFLLLLAGKAPMPKMLCFVSSLLALLLSVEPYGAVMPRAMG
                     MIVAVVSILERFRSSYGI"
     gene            1028..1435
                     /locus_tag="VM910_03195"
     CDS             1028..1435
                     /locus_tag="VM910_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVI61591.1"
                     /translation="MLYAILAYHVETEVLSWTKQEDESVMSNLRGVHDRLNRDGRLGP
                     AARLGSTTRARTLRGPGNGAVIDGPFAETKEQLLGFYLLDCTDEEAAIKAARDLRSVN
                     PSAVYEIRPIALYLPGVAIPMTEATGVVRPPKG"
     gene            1640..2851
                     /locus_tag="VM910_03200"
     CDS             1640..2851
                     /locus_tag="VM910_03200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVI61592.1"
                     /translation="MAARVYVPLSNLRAVVIMIVVAFHSVLPYLSSQPANPFPFDAAP
                     YRWVAFPIIDRERFFGFDLFCAWQDVSLMSLMFFLAGLFTPASLVRKGSLAYLIDRWW
                     RIGLPFLVAAAILSPLAYYASYRVTAADPSLEAFWRHWLALPMWPPGPAWFLWQLFFL
                     SALVACLYAIAPQSMQALDRLAGYFDDRPFAFFSSLTALSMIAYVPLAIAFSAWDWTS
                     LGPFSFQLSRPLHYVLYFFTGVALGSRGFDRGLLRHDGPLARNWLILLAAAITGFIVW
                     GEFTSLTMPDWAASVLPYRLAASAAFPPACAAGILSLLAIALRLMRFEDRRLDSLSSN
                     AYRIYLLHYVAVLWLQYALLRIALPAIAKGIIVFSLALALSWAASVGFGVLVKLLPGA
                     GSRGAMLQQRR"
     gene            2865..3998
                     /locus_tag="VM910_03205"
     CDS             2865..3998
                     /locus_tag="VM910_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61593.1"
                     /translation="MSLTLTLASRNLFHDTLRFVATMVGIVFSVVLVMVQMGLFFGFG
                     HMVTTMIDHAATDLWVVPKNVKCFEDPSLIDLKLRNKIATLDGVATVVPLVIGFSDWR
                     LESGEMTPVFVVGSDLRDGAMQPWNLVEGNVQALSQGFSVAVDRSYFDRLGVTGIGSQ
                     AEIRGKKVKVVALTDGIRSFTTTPYIFVDAKNARGFTGTPGDRASDLLVRLKPDADRG
                     TVVQAIRGQIGDAEVLTTDEFRKRSRSFWLFGTGAGAALFAGALLGVIVGTVIVAQTL
                     YSSTKDHLNEFATLRAMGSSNGYIYHVIIYQALINAVVGFAIASGIGALVVQMTAKSA
                     LPIVITNWLVAALAALTVVMCVTSALGAIFRVVRIDPAAVFMR"
     gene            3995..4750
                     /locus_tag="VM910_03210"
     CDS             3995..4750
                     /locus_tag="VM910_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61594.1"
                     /translation="MSDVIIEARGLTKRLGSGAGEVHALRGIDLSLHRGELTLLMGPS
                     GSGKTTLLLILGCMLTPTTGTVTLCGTATSGADKEGLAKLRRDHVGFVFQSYHLFPTL
                     TAAQNVQLALDIRRERGRRARKRAREALDRVGLAHKAGTRPNELSGGEQQRVAIARAI
                     VANPSIVLADEPTSSLDGDNGKTVMRLLADAAHERGQAVLVVTHDPRIVPFADRIIHI
                     EDGRIVEQPSGEAEPPRLPKAAPFEPLSAVGSQ"
BASE COUNT          816 a         1561 c         1450 g          950 t
ORIGIN      
        1 ttgatccgct gaccaagatc accaagaacc cctgcaatgg tcaagatcgc agcgggcctg
       61 aaggatcgag acctgcactt ccacgacctc cgcgggaccg cagccaccaa gttctacgta
      121 aggggcatcc ccgaacgggc gattgccgag atcatgggct gggaagagaa gcaggttcaa
      181 aagatcatca aacgttacgt cggacggacc gcctacacgg aggaactgat ccgacaactc
      241 aactcaaatc cagacgaaag gagaacatag cttgcaaaac cgtttgcaaa acgttcaccc
      301 cagaaatggg ctaagtactt ggagcgggcg aagggaatcg aaccctcgta ttcagcttgg
      361 aaggctgctg ctctaccatt gagctacacc cgcatcgctt ttcgagcatg atcttttcgg
      421 aaaaccggca ctcccccgca tcacgtgcgg ggcaggcctt ttccggatca tgctcgggcg
      481 gatgacctaa cacggcagcc atgtcgcgtt caaccgccct cgccgcccct gattcgccgg
      541 cccgcttccg caaaggcgcg tcggccccta tattaaggct ccccatcaac aacgaaagga
      601 ggtgatccag tgtcttatct caagcgctgt cacctcgctg gggtcgcccg ctaggcaaat
      661 ctgagcctgg catcggggcg ctccagccct ggaccagcga gtttttcaag agttggcgcg
      721 gcgggagcga tcccgccgcg tttactttct aaatgccgta ggacgagcga aaacgctcga
      781 ggatcgaaac aaccgcgacg atcatgccca tcgcccgcgg catgaccgca ccgtagggct
      841 cgacgctgag caagagcgcg agcaggcttg acacgaaaca cagcattttc ggcattgggg
      901 ccttgccggc gagcaacaac aagaacggca caatcatggg gcgataaaat ctgcttcaac
      961 ggacgtcggg aaccgcgggg gcgaaacgtc cttgggtcaa cccacctgtc tcgaggagaa
     1021 acgcgcaatg ctgtacgcaa tcctggccta tcacgtcgaa accgaggttc tgtcctggac
     1081 caagcaggag gacgaaagcg tgatgtccaa tctgcgcggg gtgcatgatc ggctgaatcg
     1141 ggacggccgc ctcgggcccg cggcgcgtct cggctcaacc acgcgcgccc gcaccttgcg
     1201 cggtccgggc aacggcgccg tcatcgacgg cccctttgcc gagacaaagg agcagttgct
     1261 tggcttctat ctgctcgact gcaccgacga ggaggcggcg atcaaggcgg ctcgcgacct
     1321 gcgcagcgtc aatccgagcg ctgtctacga gatccgcccg atcgcgctct atctgcccgg
     1381 cgttgcgatc cccatgacgg aagctacggg cgtggtgcgt ccgccgaagg gctagacctc
     1441 aaagcctgct cttttgccct tcgccagcgg tgattttgcg gcctgcaaag tgccgcccct
     1501 tcgccggaag gcgttgccgc cgtttgactt tttgccgatc ccaataaacc cggcggccac
     1561 ggaacgagcg caccggttcc acgttggttc catgacggtt ccacaattga caggtaccgt
     1621 cagagggaga ggtacgccgg tggccgctcg cgtctatgtc ccgctcagca atttgcgcgc
     1681 ggtcgtcatc atgatcgtcg tcgcgtttca ctcggtgctg ccctacctct cttcgcagcc
     1741 ggcaaacccg ttcccgttcg atgccgcgcc ctatcgctgg gtcgcgtttc cgatcatcga
     1801 ccgcgaacgc tttttcggct tcgacctgtt ttgcgcgtgg caggacgtca gcctgatgtc
     1861 gctgatgttt ttccttgcag gcctgttcac gccggcaagt ctcgtccgca agggctccct
     1921 cgcctatctc atcgaccgct ggtggcggat cggattgccc ttcctggttg ccgcggcgat
     1981 cctgagcccg ctggcctatt acgcatccta tcgtgtgacc gcggccgatc cgtcgctcga
     2041 agcgttctgg cggcactggc ttgccttgcc gatgtggccg ccgggcccgg cctggttctt
     2101 gtggcagctc ttctttcttt cggcgctcgt ggcatgcctg tacgcgatcg caccgcaatc
     2161 aatgcaggcg ctggatcgtc tcgccggata tttcgacgat cgtccgttcg catttttttc
     2221 gagcctgacg gcgctctcga tgatcgccta cgtgccgctc gcgatcgcgt tctcggcctg
     2281 ggactggacc tctctcggcc cgttctcgtt ccagctcagc cggcccctgc actatgtgct
     2341 gtatttcttc accggcgttg cgctcggcag ccgcggcttc gatcgcggcc tgttgcggca
     2401 cgacggtccg cttgcgcgaa actggctgat cctgctcgcg gcagccatta cgggctttat
     2461 cgtgtggggc gagttcactt ccctgaccat gccggactgg gccgcaagcg tgctgcctta
     2521 tcgccttgcc gcttcggctg cctttccacc ggcctgtgcc gccggcatcc tgagcctgct
     2581 tgcgatcgcg cttaggctga tgcgattcga ggatcgcagg ctcgacagcc tttcgagcaa
     2641 cgcctatcgc atctatctcc tccattatgt cgccgtgctc tggctgcaat atgctctgct
     2701 gcgaatcgcc cttcccgcga tcgccaaggg catcatcgtg tttagcctcg cgcttgccct
     2761 gagctgggcc gcgagcgtgg gctttggcgt gctcgtaaaa ctgctccccg gcgcggggag
     2821 cagaggagcg atgctgcagc agcgccgttg aaaggacggg tgagatgtcg ctgaccctga
     2881 cgcttgcctc gcgcaacctg tttcacgaca cgctgcgctt tgtcgcaacg atggtcggca
     2941 tcgtgttttc cgtggtcctg gtcatggtcc agatggggct gtttttcggc ttcggtcaca
     3001 tggtcaccac catgatcgat cacgccgcaa ccgacctttg ggtcgttccg aagaacgtca
     3061 agtgtttcga ggatccgtcg ctgatcgatc tcaagctgcg caacaagatc gccaccctcg
     3121 acggcgtcgc caccgtcgtg ccgctggtga tcggcttctc cgattggcgg ctcgaatcgg
     3181 gcgagatgac gccggttttc gttgtcggct cagatctgcg cgacggcgca atgcaaccgt
     3241 ggaacctggt cgagggcaac gtgcaggcgc tgtcgcaggg cttcagcgtc gcggtcgatc
     3301 gttcctattt cgaccggctc ggcgtcaccg gcatcggctc gcaggcggaa atccgcggca
     3361 agaaggtgaa ggtcgtggcg ctgaccgacg gcattcgctc cttcaccacg acgccctaca
     3421 tcttcgtcga cgcaaagaac gcgcgcggct ttacgggaac gcccggcgac cgcgccagcg
     3481 acctgctggt acggctcaag ccggatgccg accgcggcac ggtcgtgcag gcaatccgcg
     3541 gccagatcgg cgacgccgag gtcttgacca cggacgaatt ccgaaagcgc agccgctcgt
     3601 tctggctgtt cggaacggga gccggtgcgg cgctgtttgc cggcgccctg ctcggcgtga
     3661 tcgtcggcac ggtgatcgtc gctcagacgc tttattccag caccaaggac catttgaacg
     3721 agttcgcgac cttgcgcgcc atgggctcgt caaacggcta tatctatcac gtcatcatct
     3781 atcaggcgct gatcaacgcc gtcgtcggct ttgcgatcgc ctcgggcatc ggcgcgctcg
     3841 tggtgcaaat gaccgcaaaa agcgcgctgc cgatcgtaat cacgaactgg ctggtcgcag
     3901 cgctggccgc actcaccgtc gtgatgtgcg tgacgtcggc attgggcgcg atcttccgcg
     3961 tggtgcgcat cgatcctgcc gcggtgttca tgcgatgagc gacgtcatta tcgaagccag
     4021 gggacttacc aagcggctcg gcagcggtgc gggcgaggtg catgccctgc gcggcatcga
     4081 cctctcgctt catcgcggcg agctgactct gttgatgggg ccctccggca gcggcaagac
     4141 cacgcttctg ctgattctcg gctgcatgct gacgccgacc acgggcaccg tcacgctctg
     4201 cggcacggca acgtcgggcg cggacaagga agggcttgca aaactccgcc gcgaccatgt
     4261 cggcttcgtg ttccagtcct atcatctgtt tcccacgctc accgccgcgc agaacgtgca
     4321 gcttgcgctc gatatccgcc gcgagcgcgg ccgtcgcgcc cggaaacgcg cgcgcgaggc
     4381 gctcgatcgg gtgggactgg cgcacaaggc cggtacccgg ccaaacgagc tctccggcgg
     4441 cgagcagcag cgtgtcgcca tcgcacgcgc catcgtcgcc aatccgtcca tcgtgctcgc
     4501 ggacgaaccg acctcctcgc tcgacggcga caacgggaag acggtcatgc ggctgctggc
     4561 cgacgccgcc catgagcgcg gacaggcggt gctggtggtc acgcatgatc cgcgcatcgt
     4621 gcctttcgcc gatcgcatca tccatatcga ggacggccgt atcgtcgagc agccttcggg
     4681 cgaggccgag ccgccgcgcc tgccgaaggc agctcccttc gagccgctct cggcagtcgg
     4741 gtctcagtga tgagcctgcg cgccgagctg ttgcgcg
//
LOCUS       DATLJT010000147         5331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1156015,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000147 DATLJT010000000
VERSION     DATLJT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156015"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..620)
                     /locus_tag="VM910_03215"
     CDS             complement(201..620)
                     /locus_tag="VM910_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HVI61595.1"
                     /translation="MLTIGKLAAAAGVRSDTLRYYEREGLIEPAGKSPAGYRLYDKDS
                     ARRLRFIKQAQHCGFTLAEIRELLVLRGRDAACCGDVRKHAIEKKRQLEGKIRSMRAM
                     CKALDRLIAVCVDETRPVEGCAILAAFERPETTVRTN"
     gene            complement(703..969)
                     /locus_tag="VM910_03220"
     CDS             complement(703..969)
                     /locus_tag="VM910_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVI61596.1"
                     /translation="MAAKRKIEVFSAGCAACHEAIALIRRAACPSCEVIVHDMRDVEV
                     AERAKGLGIRSVPAVVIEGKLAACCAGRGVDEATLRAAGLGRPA"
     gene            complement(1118..1660)
                     /locus_tag="VM910_03225"
     CDS             complement(1118..1660)
                     /locus_tag="VM910_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI61597.1"
                     /translation="MTGRLLLILIAFALPTAASLAQSQQPYAGLEARSIKALSDQQIA
                     DLKAGRGMGLALAAELNGHPGPIHVLELADSLDLSDQQRAKMQELLTAMKAEAIPLGE
                     RLIAQEADLDKQFANKTITPASLAAATDAIGITHAALRQAHLKYHLSTVEVLTPAQVQ
                     RYGELRGYKSGIERPHGRHQ"
     gene            complement(1734..1967)
                     /locus_tag="VM910_03230"
     CDS             complement(1734..1967)
                     /locus_tag="VM910_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61598.1"
                     /translation="MRRLSWKAGLAGAALIVTTVYAVAQMTPGQMPQGPMPPEQMMGM
                     MKQMSQMMDRCSNMMQGGPHDRRDAPATPEKKD"
     gene            complement(2207..2797)
                     /locus_tag="VM910_03235"
     CDS             complement(2207..2797)
                     /locus_tag="VM910_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI61599.1"
                     /translation="MLALDQDSLERRSLARHEVLFRQGDKVTAIYFIEEGRLRLERRT
                     FDGRLLFLGTTPVGEFFVAAALFSDTFHCDAVATEPSRVRVYPKAMVLNSLRTDPASA
                     MSFLADVAHQVIELRQRLELMKVRSAKARLMLYFESNAEADGRTVRLRGQLQDIASDL
                     GLTREALYRTLASLERAGAIERAGSRILLKKSVSPR"
     gene            complement(2912..3520)
                     /locus_tag="VM910_03240"
     CDS             complement(2912..3520)
                     /locus_tag="VM910_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI61600.1"
                     /translation="MAFYRDHILPCLTHLAMSNHRLVEYRRRMVPQARGRVLEIGIGS
                     GLNLPFYGGQVASIHGIDPSAALLRRAGRQPAAPAKLVEASAEIIPFGSEVFDTVVTT
                     WTLCTIPRVVSALREMRRVLRPDGRLLFVEHGLAPDRGVAWWQHRLTPCWKCLGGGCH
                     LDRKIDDLITAAGFHIEKLDAGYMRGRNPFAFMYEGVARPAN"
     gene            complement(3589..4299)
                     /locus_tag="VM910_03245"
     CDS             complement(3589..4299)
                     /locus_tag="VM910_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61601.1"
                     /translation="MRLIPYLAFVALAIGALPYWAAAEPGNAARGERMYRACVACHSL
                     EPNRNMTGPSLAEIWNRKSGSLPSFPRYSPALKSAGIIWTDGTLDEWIKDPQHFIPGN
                     TMTFPGMKDARQRADLLAYLKDATRPGHAPKAAQGGNQMGGMMMGAGAVPNLKKLDPQ
                     DRVQSINHCGDTYKVATADGKVGDFWERNLRFKTDVTDDGPQKGAPALVGAGMMGDRA
                     DVIFAGPEEISGFISHRC"
     gene            complement(4645..>5331)
                     /locus_tag="VM910_03250"
     CDS             complement(4645..>5331)
                     /locus_tag="VM910_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVI61602.1"
                     /translation="QRLQGAGRTVVYVARAGKLIGLIAIADAPRPTAAETVAKLRERG
                     VQVAMLTGDNVGTAQRIAGELGIDIVLADVLPGQKASKVRELQAQGKKVGMVGDGIND
                     APALTQADVGFAIGAGTDVAIESADVVLMKSDPYDVVGAVELSHATLRKMHQNLWWAV
                     GYNVIAFPLAAGILYPVLLSPEIAAFAMSGSTLIVAINALMLKRTKLAGITRRNSPQT
                     SELRPAEATV"
BASE COUNT          960 a         1701 c         1620 g         1050 t
ORIGIN      
        1 cgggtgtcat tcccgttgac ttctatggat ggcttttatt ggcggttctc attcttggcg
       61 gaagcatgat catttggtgg gccacagaaa gggcgtgggg aaaattctcc tcgctaaggg
      121 actggtgact taccgcttgg atctcattcg cgcgccgact tgtctcagcc gcatcatcat
      181 gtcgactctt ctttcacagt tcagttcgtc cgcacggttg tctcgggtcg ctcgaaggcg
      241 gcaaggatcg cgcacccctc gacgggccgt gtttcatcga cacagacggc gatgaggcga
      301 tcaagcgctt tgcacatcgc gcgcatcgag cggatcttgc cttcgagctg ccgcttcttc
      361 tcgatcgcgt gcttgcggac gtccccgcag caggcggcgt cgcggccgcg caagaccagg
      421 agctcgcgga tctccgcgag cgtgaagccg cagtgctggg cctgtttgat gaagcgcagc
      481 cgccgcgccg aatccttgtc gtaaagccgg tacccggccg ggctcttgcc ggcaggctcg
      541 atcaaaccct cgcgctcgta atagcgcagc gtatcgctcc ggacgcccgc ggcggcagcg
      601 agcttgccga tggtgagcat ggcgcggcct cccacggtca gcttgaaccc tggaggtaag
      661 accaaggtca agcccgggca agcaggtgac ccatgctcgc ggctacgccg ggcgtcccag
      721 tcccgcagcc cgcagggtgg cctcatcgac gccgcgaccg gcgcaacatg cggcgagctt
      781 gccctcgatg accaccgcgg gaaccgaacg gatgccgagg cccttggcac gctcggccac
      841 ctcgacgtct cgcatgtcgt gaacgatcac ctcacacgag gggcacgccg cgcgcctgat
      901 gagcgcgatt gcctcatggc aggccgcgca tccggcactg aacacttcga tctttcgttt
      961 agcggccatc tccttgctcc catctatctt ggctctgcca tggctgaacg gtcgccgatg
     1021 gcagcgcgat ggcacaggct atcccttgga ggcagctcct aggtcaagcg tagagttacg
     1081 ggcttgaaac ggcggcttgc cagctgtccc cgtgccgtca ttggtgtcgt ccatgagggc
     1141 gttcgatccc gctcttatag ccgcgaagct cgccgtagcg ctgaacctgc gcgggcgtca
     1201 gcacctcgac ggttgacaag tggtatttga gatgcgcctg ccggagcgct gcatgggtga
     1261 tgccgatcgc gtccgtggcg gccgccaagc tcgccggggt gatcgtcttg ttggcgaact
     1321 gcttgtccaa gtccgcctct tgtgcaatca gccgctcgcc gagcggaatc gcctcggcct
     1381 tcatggctgt gagcagctct tgcatcttcg cgcgctgctg gtcggataga tcgagtgaat
     1441 ccgccagttc tagcacgtgt atcgggccgg gatgtccgtt gagctccgcc gccagcgcca
     1501 agcccatgcc gcgccccgcc ttcaaatcgg caatttgctg gtcggagagc gccttgatgg
     1561 aacgtgcctc aagtcccgca tagggctgct gcgactgcgc caacgaagcg gccgtcggca
     1621 gagcgaaggc aatcaagata agaagcaagc gaccagtcat ctgtgcttct ccctactttc
     1681 cgacttgaaa ctaggactgg tcggcccctc gcaccgatat attgggccgg ccttcagtcc
     1741 ttcttctccg gcgtggcagg cgcgtccctg cggtcgtgcg ggccgccctg catcatgttg
     1801 ctgcagcgat ccatcatctg gctcatctgc ttcatcattc ccatcatctg ctccggcggc
     1861 atcggcccct gcggcatttg tccgggggtc atttgcgcga ccgcgtagac ggtcgtgacg
     1921 atgagggcag caccggcaag gcccgccttc caagacaatc ggcgcatgat ttatctcctt
     1981 ggtcagtggc ggtcggcata tgcccaaata aggatccgcc atatgattgg aatcatgcgc
     2041 tatcctttgg cgcacgatcg cgcaggcggg gtcagggaag gcgttcgacc atgatgcaaa
     2101 tgaccgactt gccgataacg atgatgtggg gaatgggctt catttggctc ctcttcatca
     2161 tcttcctcgt tctggggatc gccgcgttca tcaagtatct gcgctcctag cgcgggctga
     2221 ctgatttctt cagcaggatg cggctgcccg cgcgttcgat cgcgcccgca cgctcgaggc
     2281 tggcaagggt ccgatacaga gcttcgcggg tcaggcccag atcgctcgca atatcctgca
     2341 gctggccgcg cagcctgacc gtgcgtccgt cagcttcagc gttggactcg aagtagagca
     2401 tcagccgtgc tttggcggag cgaaccttca tgagttcaag gcgctgccgc agctcgatga
     2461 cttgatgcgc cacgtctgcc aggaacgaca tggcgctcgc cgggtcggtg cgaagagaat
     2521 tcagcaccat cgccttggga tatacgcgca cccgtgaggg ctccgtcgcc acggcgtcgc
     2581 aatggaatgt atcggaaaag agcgccgctg cgacgaagaa ctcgcccacg ggtgtggtgc
     2641 cgaggaacag caatcgaccg tcgaaggtgc gccgttccag ccgcagccgc ccttcttcaa
     2701 tgaaataaat ggcggtcact ttatcgccct gccgaaacaa cacctcgtgt cgagcgagcg
     2761 atcgccgttc aagcgagtct tggtcgaggg ccagcatgct catatccagg catcaaccgc
     2821 taaagcgtgc aactggtgtt ccgtggagcg atcacgttcc catttcgccg ggggtgggcg
     2881 gggctcccca ggtcgcacgc ccaatgccgg ctcaattggc aggccgcgcg acgccttcgt
     2941 acatgaaggc aaacggattt cgccctcgca tgtatccggc gtcgagcttt tcgatgtgaa
     3001 atcccgccgc cgtgatgaga tcatcgatct tgcggtcgag atgacaaccg ccgccgaggc
     3061 atttccaaca cggtgtcagg cgatgttgcc accatgccac tccgcggtcg ggcgccaatc
     3121 cgtgctccac gaacaacagg cggccatccg gacgaagcac gcgccgcatt tcgcgaagcg
     3181 cggagacgac tcgagggatc gtgcacagcg tccacgtggt caccactgtg tcgaaaacct
     3241 cgctaccgaa tggaatgatt tcggccgacg cctccaccaa tttcgctggg gcagccggtt
     3301 gcctgccagc tcgtcgtaac agcgcggctg acggatcgat gccgtgaatg ctcgccactt
     3361 gcccgccata gaacggcagg ttcaagccgg acccgatccc gatctccagc acgcgaccgc
     3421 gggcctgcgg caccatccgc cgccggtatt cgacgagccg gtggttcgac atcgcgaggt
     3481 gagtgaggca cggcaggata tgatcgcgat agaacgccat gacgtttcag cacgctcgcc
     3541 ggccgatgca atattcggtg tctttccgcc tattacggga gccgcggttc aacaccgatg
     3601 cgagatgaag ccgctgatct cctcgggtcc ggcgaaaatc acgtcggcgc ggtctcccat
     3661 cataccggct cccacgaggg ccggcgcgcc cttttgtgga ccatcgtcgg tgacatcggt
     3721 cttgaagcgc aagttgcgtt cccagaaatc gcccaccttc ccatccgcgg tggcgacctt
     3781 gtaggtatcc ccgcaatgat tgatcgattg cacgcgatcc tgcggatcga gctttttcag
     3841 attcggcacc gcgccggcgc ccatcatcat gccgcccatc tgattgccgc cttgtgcagc
     3901 cttgggcgca tggccgggac gggttgcatc cttgagatat gccagcagat cggcgcgctg
     3961 ccgcgcgtcc ttcatgccgg gaaacgtcat ggtgtttccc ggaatgaagt gctgcgggtc
     4021 cttgatccat tcgtcgagcg tgccgtcggt ccagatgatg ccggccgatt tgagcgcggg
     4081 ggaatagcgc gggaagctcg gcaacgatcc ggactttcgg ttccagatct ccgcgagact
     4141 cgggccggtc atgttgcggt tgggctcgag cgaatggcat gcgacgcagg cgcgatacat
     4201 gcgctcgccg cgcgcggcat tgcccggctc cgcggcggcc caatagggca gggcaccgat
     4261 cgccagcgca acgaacgcga gatacgggat gagcctcatc gtcgacctcc atcggtctgc
     4321 ttgttttgtc ctccccctgt gatcgcaaac gctacggccg gcacgacgat ccactgcagc
     4381 aacggcgaga gaccgatgct aagacccgca atcggaatga ccggcatcag gagcaaggcg
     4441 gaagcgacct ggtccgcgtg cggccctcgc ggacgagtcc ctgggcgtgt cccaaccggc
     4501 accttgccga gcatggtcac cggcggcctc taggaatgga gatatttgat ggccgccgct
     4561 atcccaagca aagcgaagat gaagatcgcg aaatgatcat tccaaccgac atcgtcgtga
     4621 cgtccattat caataagact gccttcacac cgttgcctcc gctgggcgca attccgacgt
     4681 ttgggggctg ttgcgccgcg tgatgccggc tagtttggtg cgcttgagca tcagcgcgtt
     4741 gattgcgaca atcagcgtgc ttccagacat ggcgaacgcg gcaatctcgg gactgagcaa
     4801 gacgggatac aagatgccgg cagccaatgg gaaagcaatg acattgtagc cgactgccca
     4861 ccacaggttc tggtgcatct tgcgcaaggt ggcatgggac agctcaaccg cgcccacgac
     4921 gtcgtaggga tcactcttca tcagcacgac gtccgcgctc tcgatggcga cgtcggtgcc
     4981 tgcgccgatg gcaaagccca catccgcctg cgtgagcgcg ggtgcgtcat tgatgccatc
     5041 gccgaccatc ccaaccttct tgccctgagc ctgcagctcc ctgactttgc tcgccttctg
     5101 accgggcagc acatcggcaa gcacaatgtc gataccaagt tctccagcaa ttcgttgcgc
     5161 cgtcccgacg ttgtcaccgg tgagcatggc gacctgcact ccccgctcgc gcaacttggc
     5221 gacggtctca gccgcggtcg gccgcggcgc gtcggcaatg gcgatgaggc caatcagttt
     5281 tccggcacgt gccacgtaca ccacggtgcg acccgcacct tgcagccgct g
//
LOCUS       DATLJT010000148         2511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2304750,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000148 DATLJT010000000
VERSION     DATLJT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304750"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VM910_03255"
     CDS             complement(<1..383)
                     /locus_tag="VM910_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI61603.1"
                     /translation="MRFDRSIGLCLGATLLCGSSLLNPVLAKPFMIVGLDEKVWWDDD
                     GKGVLSLPGKDQVLIVDVADPENPKIVTSLPLENSIVGPPVNLDINPAGTVALVADSV
                     DVVKEGDALKQVPDNKIYVIDMTANP"
     gene            580..1386
                     /locus_tag="VM910_03260"
     CDS             580..1386
                     /locus_tag="VM910_03260"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI61604.1"
                     /translation="MDRLKGKTAMVVGAGSIGPGWGNGKATAVTFAREGAQVFCVDRN
                     SAAVEETAGIITKEGGKARSFTADVSRAGDVEAMVKACLAAFGRIDVLDNNVGIAEVG
                     SVVEVDEAEWDRVFAVNLKSAYLAMKHVIPVMVGQGGGSIINISSIASIRHVGISYVT
                     YGTTKAAMNQMTRTTAVEFAAKHVRVNAILPGLMKTPMVEHSAGLAQSYAKGDVEAMW
                     RARDAQVPMGHMGDAWDVANAALFLASDESKYVTGIELVVDGGITCKSGG"
     gene            1531..2247
                     /locus_tag="VM910_03265"
     CDS             1531..2247
                     /locus_tag="VM910_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61605.1"
                     /translation="MLPGFAYAAGTETDTAPLADPSRCQSAIAANDDDRILAECGALI
                     DNEKTAKADRIKVLIARAAVFTRKDQIERAIADYDSALQLDPTLADIYNTRGELWWKK
                     GDRPRALADFAAAIKLNPDHAAAKGNYKSLAQELERLGALMAVAGKPSFDCATARRGV
                     EKAICANPELADLDREINAVNTRVVRAAAANSPRAGRALQQQQDEFLARRNASFGRAD
                     YDLQKAMRERLDQLLAIARQ"
BASE COUNT          483 a          769 c          827 g          432 t
ORIGIN      
        1 ggattggccg tcatgtcgat cacgtagatc ttgttgtcgg gcacctgctt cagcgcatcg
       61 ccctccttga ccacgtcgac ggagtcggcg accagggcga cggttccggc ggggttgata
      121 tcgaggttga ccgggggacc cacgatcgaa ttttccagcg gcaggctcgt aacgattttc
      181 ggattctcgg ggtcggcgac gtcgacgatc aggacctggt ccttgccggg aaggctcagc
      241 acgcccttgc cgtcgtcatc ccaccacact ttctcgtcga gtccgacgat catgaacggc
      301 ttggcaagga caggattgag caggctcgag ccgcagagca gcgtggctcc aaggcaaagt
      361 ccaatgctac gatcaaagcg cataatggcg ttctccccga tatgactggc ctctggcggg
      421 ccggttggcg gcggtgagac tacgcgggga tggccgcagt ttccagtgcc atggcggtcg
      481 cccttggccg cacgctccgg atattttcag acaagggatc ggtgctaatg tcgccgcgcc
      541 gcacgtgcga agcgagaaga aacgaagcag gagaggacga tggaccggct caagggcaag
      601 acggcgatgg tggtcggcgc gggctcgatc ggccccggtt ggggcaacgg caaggcgacg
      661 gcagtcacct ttgcgcgcga gggagcgcag gtgttttgcg tcgatcgcaa ctccgccgca
      721 gtcgaagaga cggcgggcat catcacaaag gagggcggca aggcgaggtc ctttaccgcc
      781 gatgtttccc gggccggcga tgtcgaggcg atggtgaaag cctgccttgc ggccttcggc
      841 cggatcgacg tgctcgacaa caatgtcggc attgccgagg tgggcagcgt ggtcgaggtc
      901 gatgaagccg aatgggaccg cgtcttcgcc gtcaatctca agagcgccta tcttgccatg
      961 aagcacgtca ttcccgtgat ggtcgggcag ggtggcggct cgatcatcaa tatctcctcg
     1021 atcgcctcga tccgtcacgt cggcatatct tatgtcacct acggcaccac caaggccgcg
     1081 atgaaccaga tgacgcgcac cacggcggtc gagttcgcgg cgaagcatgt ccgggtcaac
     1141 gcgatccttc cggggctgat gaagaccccg atggtcgaac actccgcggg acttgcgcag
     1201 agttacgcca agggcgacgt cgaggcgatg tggcgcgccc gcgacgcgca ggtgccgatg
     1261 gggcacatgg gcgacgcctg ggatgtggcc aacgccgcgc tgtttcttgc ctccgacgaa
     1321 tcgaaatacg ttaccggcat cgaactcgtg gtcgacggcg ggatcacctg caagtcgggc
     1381 ggctagttct gcgcgattgc gccattgcca ggatgccgcc ggcgaaggag tgccacaccc
     1441 gacacgctga tcggcagatt aagcagcttg atgcaactga ggcttaccgt ctcctttcgt
     1501 gttgcctttg ccgcggcggc gctggggaca atgctgcccg gcttcgcgta tgcggctggc
     1561 acggagaccg acacagcacc gctcgccgat ccgtcgcgct gccagagcgc gatcgcggcc
     1621 aatgacgacg acaggatttt ggccgaatgc ggcgcgctca tcgacaatga gaaaaccgcg
     1681 aaggccgacc gtatcaaggt gctgattgcg cgcgcggcgg tcttcacccg taaggaccag
     1741 atcgagcggg ccatcgccga ttacgatagc gcgctgcaac tcgatccgac gctcgccgac
     1801 atctacaaca cgcgcggcga gctgtggtgg aagaagggcg accggccgag ggcgcttgcc
     1861 gatttcgccg ccgccatcaa gctcaacccg gatcatgcgg ccgcaaaagg caattacaag
     1921 tcgttggcgc aggaactgga gcggctgggc gcgctgatgg cggtcgccgg caagccgagc
     1981 ttcgattgcg ccaccgcccg ccgcggcgtg gagaaggcga tctgcgccaa ccccgagctt
     2041 gccgatctcg accgcgagat caatgcagtg aataccaggg tggtacgcgc cgcggccgcc
     2101 aacagcccgc gcgcgggacg cgcgctgcag caacagcagg atgagttcct ggcccgccgc
     2161 aacgcctcct tcggccgagc ggactacgac ctgcagaagg cgatgcgcga gcggctcgac
     2221 caactgcttg caatcgcgcg gcaataaaac gcgttccgga aaaagcctgc ccgctcgggt
     2281 agcggggtgg gggccgccct cgaactactt gatcaggagg gaacgcgttg aaacaacaag
     2341 ccaaagcgcg acggcgatct catgccttcg cgctttagaa cttttccggc ttgaatccgc
     2401 gccgcttcgc gcgacaatac tctcaaaaat ccggcacgga tttgatcctg atcatgggct
     2461 gatgccgccg gtcatcggat ttttgcattc gcaccccctg cgggggcagg c
//
LOCUS       DATLJT010000149         4854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4142731,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000149 DATLJT010000000
VERSION     DATLJT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4142731"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1027
                     /locus_tag="VM910_03270"
                     /pseudo
     CDS             266..1027
                     /locus_tag="VM910_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            1641..>4854
                     /locus_tag="VM910_03275"
     CDS             1641..>4854
                     /locus_tag="VM910_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI61606.1"
                     /translation="MSRKGANSPTRGRKLRSTGTKAAAHVSDGPNSLIELKKQLEART
                     RELAEARGHLSEALERQTATSEILQVISSSPGDLQPVFETILANATRICEAKFGSMYL
                     SEGDAVRIVAMHNAPPSFAEERRRNPLIRPSPDTTFGRALATKQTVQIADVQNEPAYV
                     NAPSGFTGAQLAKLAGARTVVVVPMLKESDLVGVIIIYRQEVRPFTDTQIELVSNFAR
                     QAVIAIESTRLLNELRESLQQQTATSEVLQVISSSPGELEPVFQAMLSNAVRVCEAKF
                     GSLYLYDGDRFHVGALHNAPPAWAEFRRREPVFHPPPGTGLAQMVATKRTFQTLDMTL
                     ERGYLDRNPIIVASVELGGFRTALAVPMVKDDKLAGVISVYRQEVRPFTGKQIALVTN
                     FAAQAVIAVENTRLLNELRQRTDDLSEALEQQTATSQVLQVISSSPGALQPVFESMLE
                     HATRICEAAFGSMLLREGNMFRRVALHNAPQEYAQLHQKEPLFGTSQTLARLLAAKQA
                     AQVADMAVAEPAAPIAGLGGARTLLNVPMIKEGEVIGVIGIYRQEVRPFTDKQIELVT
                     NFAAQAVIAIENTRLLNELRESLEQQTATSEVLEVISSSPGELEPVFTTMLQNALRIC
                     EAGIGNLFRYEDGAFREASSVNAPTAFMQFLRRGPVQPTPGTGLARVVQTKQTAHIRD
                     IRELEAYANRDPFVVAGVEAGIRTLLVVPMLKDNALLGVIGIYRLEVRPFTDKQIELV
                     TNFARQAVIAIENTRLLNELRESLQQQTATADVLKVISRSTFDLKAVLKALVESAARL
                     CEADMASINRPIGNTYHREASCGFTAELNEHMAGVVPQPGPGTVVGRALLGRKIVHIL
                     DAAADPDYTLVEARRIAGFRTMLGVPLLREGVPVGVIMLARRTVRPFTDKQIGLVSTF
                     ADQAVIAIENVRLFDEIQDKSRQLADASERKSQFLASMSHELRTPLNAIIGLTEMMVS
                     NAARFGTEKALEPLRRVNAAGTHLLSLINEILDLSKIEAGKLELNPERVNLARLIDEV
                     IGTAGQLAEKNKNRLVVEAQENVGSLTADSLRLKQILLNLL"
BASE COUNT         1022 a         1458 c         1461 g          913 t
ORIGIN      
        1 gtgacgacaa tcagcttttc gcattgctga atgtacttca gcagggtgtc gaacaacgcg
       61 cccacaagat actcatagag cgagaccgcg cggaggctcc gcgcgagcca gtcccaacga
      121 gacgacttta ccaaagcgca agcggagacg aacggaagcc gctggacgcg gcaaccgaaa
      181 gcggccccga gaaatactcg acgccgagag aattggccta aacccctgat attattgcgc
      241 ttaagcgaaa ggcgagaacc ggccgggcgg cggcaccaac atcggcaccg aggcggtcgt
      301 gcgcgcatcg ccggacggct atacgctgct cgtggtcggt gtgccgagcg cgatcaacgc
      361 gacactttac gataggctga atttcaattt catccgcgac atcgcgccgg tcgcgagctt
      421 tgccagcggc gccttggtca tggtggtcac tccgtcgttt ccggccaggt cggtgcccga
      481 attcatcgcc tatgccaagg ccaatccggg caagatcagc atggcatcag ccggcagtgg
      541 ggcgccgtct catgtctccg gtgacttgtt caagatgatg gccggtctcg atctggtcca
      601 tgtgccctat cgcggcggtg ggcccgcggt cgctgacctc ctcggtggcc aagtggacgt
      661 gaccttcgct gtcgtgacca cggctatcga atacatcaga gccggcaagc tgcgggcgct
      721 ggcggtgacc agcgcgacgc gccaggaggc tttgccggat atcccgaccg tggccgattt
      781 cttgccaggc tacgaggcaa gcgggtggtt tggccttttc gcgcccagga acacgccggc
      841 cgaaatcatc gacgggctca acaaggaaat taacgcggcc ctcgctgatc ccaaggtgaa
      901 ggcgcggctt gccgatcttg gcggcacggt ggttctcggc ttgcccgccg acttcggaag
      961 gctcgtcgta gatgacaagg aaaagtgggc gaaggtgatt cgggcagccg gcattaaagc
     1021 ggagtgaccg ccaaccgaat attccgtaac gtccgttgaa ctaagccgct gtggcggcat
     1081 tggagatcgt tgcaaggtcg ggaggtccgc tggcatgatc acggtgcgac gagacgcagt
     1141 gaggacgcaa ggccgtgcac cgaagggcgg aggagatcaa cgagaccacg aagcagtggg
     1201 ttcgtcattg cggcttgaag ctcgtcttcg atcacgcaga ggttgtcaaa tgcgacattt
     1261 ctgccacagc taacactttg aaatcacagg acacgccaaa cctactttcc cctattagca
     1321 acattcgtgc cgtaacgccc aagttgtact cgcgagcacc gtcgtcacgg gtcgaaattc
     1381 gtccgtgctg aaagtctccc tcgctgctcg acagtgccag cggcttcgtt caacgaaggt
     1441 tcctataagg tcgcgctcat ctgcgtgttc gaggctcgtc tccgccgagc gacctcatag
     1501 aaacctcacc gttaggcgaa tggcggccgc caaggtgatc gaatgaacgg gcgcgagttc
     1561 atcgtgcggt tctgttagga ttgataggct cgatccttag ctgggccgac gagcccagtg
     1621 gctgtgccga cgaggtgatc ttgtctcgca aaggcgcaaa tagcccaacg cgcggccgca
     1681 agcttcgttc gaccggaacg aaggcggcag cgcacgttag cgacgggccc aattcactga
     1741 tcgagttgaa aaagcaactc gaagcgcgca cccgtgagct cgccgaggcg cggggacacc
     1801 tttccgaggc gctggagcgg cagaccgcga cgtcggagat actgcaggta atatcaagct
     1861 cccccggcga tctgcagccc gtgttcgaga caatactggc gaacgcaacg cgcatctgcg
     1921 aggccaagtt cggctcgatg tacctcagtg agggagacgc cgtccgcatc gtcgcgatgc
     1981 ataatgcacc gccgtcgttt gccgaagaac ggcggcggaa cccgttaatc cgtcccagcc
     2041 ccgatacgac gttcggacgc gcattggcga cgaagcagac ggttcagatc gctgatgtcc
     2101 aaaacgaacc ggcctacgtc aacgcgccgt caggcttcac gggcgcacag ctcgctaagc
     2161 tcgccggcgc tcggaccgtg gttgttgtcc cgatgctcaa ggaaagcgat ctggtcggtg
     2221 tcattatcat ttaccgccag gaggtccggc cgttcaccga cacgcagatc gagctggtca
     2281 gcaattttgc gaggcaagcg gtcatcgcca tcgagagtac gcggctgctc aacgagctgc
     2341 gcgaatcgct gcagcagcag accgcgacgt cggaagttct tcaggtgatt tccagttcgc
     2401 ccggcgaatt ggaaccggtg ttccaggcca tgctttcaaa cgcggtgcga gtctgcgaag
     2461 cgaagtttgg ttcgctttac ctctacgacg gagacagatt tcacgtcggc gcactacaca
     2521 acgcgccacc cgcctgggct gagtttcgca ggcgtgaacc ggtgtttcat cccccgccag
     2581 ggacgggtct ggcacagatg gtcgcaacaa agcgcacgtt tcaaaccctc gacatgacgc
     2641 tggagagggg ctacctcgat cgaaatccaa tcatcgttgc gagtgtcgag ttaggcggtt
     2701 ttcggaccgc gctggctgtt cccatggtga aggacgacaa gctggccggc gtgatcagcg
     2761 tctaccggca ggaagtgcgc ccctttaccg gcaagcagat cgcgctggtc acgaacttcg
     2821 cggcgcaggc cgtcatcgcc gtcgagaaca cgcggctcct aaatgagctg cgccagcgca
     2881 ctgacgatct ctctgaggca ttggagcaac agacggcgac ttcccaggtc cttcaggtta
     2941 tcagctcttc gcccggcgca ttacagcctg tctttgagtc aatgctggaa catgcgactc
     3001 gcatttgcga ggccgcgttc ggctcaatgt tgctgcgcga ggggaacatg tttcggcgcg
     3061 tcgcgctgca caatgcgccg caagagtatg cacaattaca tcaaaaggag ccgctttttg
     3121 gcacttctca gacgctcgcc cgtcttctgg cagcaaagca ggcggcccag gtcgccgata
     3181 tggccgtcgc tgagcccgca gcaccgatag ctgggcttgg cggggcgcgc acgttgctga
     3241 atgtgccgat gatcaaagag ggcgaggtca tcggcgtaat cggtatttat cgtcaggagg
     3301 ttcgcccgtt cacggataag cagatcgaat tggtaacgaa tttcgccgcg caagctgtaa
     3361 tcgccatcga gaacacacgc ctgctcaacg agctgcgcga atccctggag cagcagaccg
     3421 cgacctctga ggtcctggaa gtgatctcca gctcgcccgg cgagctcgag ccggtgttca
     3481 cgacaatgct gcagaacgcc ctgcggattt gcgaagccgg catcggcaat ctgttccgat
     3541 atgaggatgg ggcgtttcgc gaggcttcaa gcgtcaacgc cccgacagcc tttatgcagt
     3601 ttctccgacg aggtccggtt caaccaaccc ccggcaccgg tctcgcgcgc gtggtccaaa
     3661 caaagcagac cgcgcatatc cgcgacatta gagagctcga agcctatgca aatcgtgatc
     3721 catttgtagt cgccggcgtt gaagcaggca tccgaactct ccttgtcgtg ccgatgctca
     3781 aggacaacgc gctactcggt gttatcggca tctaccgcct cgaggttcgc cccttcacag
     3841 acaagcaaat tgaactggtc acaaatttcg cccgccaagc ggtgatcgct atcgagaaca
     3901 cccgcctgct caacgagctg cgcgaatcac tgcagcagca aacggccacc gccgacgtgc
     3961 tcaaggtcat cagccgctcg accttcgacc tcaaagcggt gctcaaggcg ctggtcgagt
     4021 cggccgcccg cctctgcgag gcggacatgg catcgatcaa ccggccaatc ggcaatacct
     4081 atcatcggga agccagttgt ggtttcaccg ccgaattgaa cgaacatatg gcgggtgttg
     4141 ttccacagcc tgggcccggt acggtcgttg gacgggcgct gctaggacgt aagatcgttc
     4201 acatcctcga tgcagccgct gacccggact acacgctagt cgaagcccgt aggattgcgg
     4261 gattccggac catgctcggc gtcccgctgc tacgcgaggg ggtgccggtc ggcgtcatca
     4321 tgttggcgcg tcggacggtg cgtccattca ccgacaagca gatcgggctg gtttccacct
     4381 tcgccgacca agcggtgatc gccatcgaga acgtgcggct gttcgatgaa atccaggaca
     4441 aaagccggca actcgcggat gcgagcgagc gcaagtcgca gttcctcgcc agcatgagtc
     4501 acgaactgcg gacgcccctc aatgccatca tcggcctgac ggagatgatg gtcagcaatg
     4561 ctgcacgctt tggcacggaa aaagcgctgg agccgctgcg gcgtgtgaat gccgccggca
     4621 cccaccttct cagcctcatc aacgagatac tggacctctc gaagatcgaa gccggcaagc
     4681 tcgaactaaa tcccgagcga gtgaatttgg cccggcttat cgatgaggtc atcggcaccg
     4741 caggacagct tgccgagaag aacaagaatc gcctcgttgt cgaagcgcaa gagaacgttg
     4801 gttcgctcac cgcggactca ctgcggctga agcagattct gctcaatctc ctaa
//
LOCUS       DATLJT010000150         3973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3453434,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000150 DATLJT010000000
VERSION     DATLJT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453434"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..583
                     /locus_tag="VM910_03280"
                     /pseudo
     CDS             1..583
                     /locus_tag="VM910_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612218.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(608..2680)
                     /locus_tag="VM910_03285"
     CDS             complement(608..2680)
                     /locus_tag="VM910_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI61607.1"
                     /translation="MTTHDVLLPVTVLQRKAVVYVRQSTPQQVQSNLESQRRQYELVE
                     VARRRGFTSVEVIDDDLGRTASGAVERPGFDRLVAALCAGQVGAVLCLDASRLARNGR
                     DWHHLLELCGLVEARVIDLDGVYDPCLPNDRLLLGMKGSISEFELGIIRSRMYEAARS
                     KARRGELRISPPIGYTWDRHVGLGLDPDRRLQEVIRLVFQKFRELGSARQVLLWMASQ
                     NIHFPYPSDGRTLTSIEWRPIRYRNVISILKNPFYAGVYSYGKSEKRTTLVDGRARKS
                     YGHRKPMDAWEVFIKDHHEGYIGWTEYERNQALLAGNAYGRVGDTKSGRGGRALLAGL
                     ICCARCGRRLNVVYNGRYPRPVYRCDKPNLQLGRRRCFSFGGKRIDETIAAEMLRAVA
                     PMAIEAAMEAERMLKEEDHDRQRIAELELQQAQYDASLAERRYAACDPDNRLIAAQLE
                     RAWETALERVEACRQRLDGMRAPDVGDARPDFTGLADDLSAAWKAPRTTMRTRQRLVR
                     ALITEIIADVDEKAGDIVLVIHWKGGQHSELRVRKPKTGEHGCSTPEQALAVMRSMAG
                     RWSDQDIAASLNRMGMPTGQGKTWTAHRVGSLRRVHGIHGYRSADKNSEWLTLRDAAA
                     KLGVSHHQVRKLIKAGVLASEQIMPDAPHQIRAAELESERVAAALKRKGRPCRGGPEN
                     QTSMFPDT"
     gene            complement(2667..2885)
                     /locus_tag="VM910_03290"
     CDS             complement(2667..2885)
                     /locus_tag="VM910_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017266853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61608.1"
                     /translation="MGSLVPLARRTQLVLRPECTPPTAPRQLDLVLDDVRLRGMTPAE
                     RQAVLRSLAGLLLEAGGVGMWEVGDDNA"
     gene            3201..3416
                     /locus_tag="VM910_03295"
     CDS             3201..3416
                     /locus_tag="VM910_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HVI61609.1"
                     /translation="MLGRKNHLFAGSDGGAERWATVCSLIASAKLNDVEPFAYLKDTL
                     QRMTEGHPMRRLDELLPWNWSKIAVKR"
     gene            complement(3631..>3973)
                     /locus_tag="VM910_03300"
     CDS             complement(3631..>3973)
                     /locus_tag="VM910_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI61610.1"
                     /translation="KAKAAVTQKIHMDIASKTAHLPGRVILVRGFLFFGWMVAFLCSM
                     AVIGLIPTVPIFVIAYMRLEGPEKWRHAALMAAVMTTLIYVVFDQLLNIPWPPTLLGT
                     SFPVFKFIPSV"
BASE COUNT          736 a         1280 c         1194 g          763 t
ORIGIN      
        1 ggtgctggat cccggccgcg gtcgcaccaa gaccggccga ttgtgggtct acacgcggga
       61 cgatcgccct tggggtgggc ccgatccgcc ggcggcggtc tacttctaca gtcccgaccg
      121 caaggcggag cggcctgcct cacacctcga agcattccgt ggtgttctgc aggttgatgg
      181 ctatgccgga ttcgagcggc tgactgagcg tggtgagatc gtgctggcag cctgttgggc
      241 gcacacgcgg cgcaagttct ttgatgtgca tgaggcgacc ggctcgccaa ttgcaggcga
      301 agccttgcgc cgcattgccg agctgtatgt aatcgaaagg tcgatccgag gccgcgccgc
      361 cgaagagcgc cgaagcgtgc gtgattcccg atcacggccg cttgtcgagg cgatgaagcc
      421 ttggctcgag gcgcagcttg gtcgcattcc tggccgcagt ggcctcgccg acgcgatccg
      481 gtacaccctt tcccgctggc cggccctctg tcatttcctg gacgacggtc gtatcgagct
      541 cgacaacaat tccgtcgaac gcgcgatccg gcccgtcgcg atgcctgttt cattctgcac
      601 tccctcttca agtatctgga aacattgagg tctgattttc tggaccgccc cgacacgggc
      661 gaccttttcg cttgagcgcg gctgcaaccc gctcgctttc gagctcggcg gcacggatct
      721 ggtgaggtgc gtcgggcata atttgctcgc ttgcgaggac gccggccttg atgagcttgc
      781 gaacttgatg atggctgacg ccgagctttg cggccgcatc gcgcagcgtc agccactcgc
      841 tgttcttatc ggcggagcga tagccgtgga tgccatgcac gcggcgcaac gacccgacac
      901 ggtgcgccgt ccaggtcttg ccctggccgg tcggcattcc cattcggttc agtgatgcgg
      961 cgatatcctg atcggaccat cgaccggcca tgctgcgcat gacggcgagc gcctgctcgg
     1021 gtgtgctaca gccatgctcg ccggtttttg gcttgcggac gcgcagctct gaatgttgcc
     1081 cacccttcca gtggatgacg agcacgatat cgccggcctt ctcatcgaca tcggcgatga
     1141 tctccgtgat cagagcccgc accagtcgct ggcgcgttcg catcgtcgtt cttggcgcct
     1201 tccatgccgc cgagagatca tcggccagtc ccgtgaagtc gggacgagcg tcgccgacgt
     1261 cgggagctcg cattccatcg agccgctgcc gacacgcttc gacacgctcc agcgcggtct
     1321 cccacgccct ctccagctgc gcagcgatca agcgattgtc ggggtcacaa gcggcatagc
     1381 gacgttcggc aagagatgca tcgtattgag cctgttgcag ctccagctct gcgatacgct
     1441 gtcggtcatg gtcctcttct ttcagcatac gctcggcctc catcgctgct tcgatcgcca
     1501 ttggcgctac ggcgcgtagc atctcggcag cgattgtctc gtcgatacgc ttgccgccaa
     1561 agctgaagca acgccgccgc cccagctgca ggttcggctt atcgcatctg tagacaggcc
     1621 gcggatagcg tccgttgtag acaacattga gccgacggcc acagcgtgcg caacagatga
     1681 gaccggcgag cagtgcgcgc ccgccgcggc cggacttcgt gtcgccgacc ctgccgtagg
     1741 cattgcctgc gagcagcgcc tgattgcgct cgtactccgt ccagccgatg tagccttcgt
     1801 gatgatcttt gatgaacacc tcccatgcgt ccatcggctt acggtggccg tagctcttac
     1861 gcgctcggcc atcgacgagc gtggtgcgct tctcgctctt cccgtaggag tagacgccgg
     1921 catagaaggg gttcttgaga atcgagatga cgttacgata cctgatcggc cgccattcga
     1981 tcgacgtcaa cgttctgcca tcggacggat agggaaagtg gatgttctgc gacgccatcc
     2041 acaatagcac ttgtcgggcg ctgcccagct cgcggaactt ctgaaagacg agccttatca
     2101 cctcctgcag ccggcgatcc ggatcgaggc cgagaccgac atgccgatcc caggtgtagc
     2161 cgatcggcgg agagatcctc agctcgccac gacgcgcctt cgatcgtgcc gcctcgtaca
     2221 ttcgagatcg gatgatcccc agctcgaact cgctgatgct ccccttcatg ccgagcagca
     2281 ggcggtcgtt cggtaggcac ggatcgtata cgccatcgag gtcgatgacg cgggcttcca
     2341 ccaatccaca tagttcaagc agatgatgcc agtcacggcc attgcgagcg agccgcgagg
     2401 catcgaggca gagcacggct ccgacctgcc cggcgcacag cgcggcgacc agccgatcga
     2461 agccgggccg ttccacggcg ccgcttgccg tgcgaccaag atcgtcatcg atcacttcaa
     2521 cgctagtgaa gccgcgccga cgtgcaactt cgaccagctc gtactggcgc ctttggctct
     2581 ccagattcga ttgcacttgc tgcggcgtcg actggcgcac atagacgacc gccttgcgtt
     2641 gcagcaccgt taccggaagg agaacgtcat gcgttgtcat cgccgacctc ccacattccg
     2701 acgccccccg cctcgagcag gagacccgcc agcgacctga gcacggcctg gcgctccgcc
     2761 ggtgtcatac cccgcagtcg gacgtcgtcg agaacgaggt cgagttggcg tggcgccgtc
     2821 ggcggcgtgc actccggcct cagaacaagc tgcgttcttc gtgcaagcgg taccagcgat
     2881 cccatcatga gcctcctgcg tgacggtgtt gccgtccgcg gagaccagcc acgattcgca
     2941 agcgaccagc agctcatgca atgcgccgag cgccgccaac gagacctgcg gggcaccgac
     3001 cttgagcccc gcgcaataga ccacatcgag cttccatgcc ggcagaatcg tcacgacgcc
     3061 cggcgccagc tccacgtgca cgagctcacc cgaagcgcgc cgctcgatct gaatgcgacg
     3121 cgcgctttgc ccataaagcg gatgccagcg atagtggatg acgacattct gcccgatatg
     3181 ggcagaatgt ctgcgagatt gtgctcgggc gcaagaacca tctgttcgcc ggctcggatg
     3241 gtggcgctga gcgatgggcc accgtctgct ccctcattgc ctctgcaaag ctcaacgacg
     3301 tggagccctt cgcctacctc aaggacaccc tacagcgcat gaccgaaggc catccgatgc
     3361 gccggttaga cgagctgctg ccgtggaatt ggtctaaaat cgccgtcaag cgttaaatga
     3421 cgtgcgatcg tcggacgctt acgaaggatc agcgaaagct gcggaccttg aacagcgatg
     3481 ctaacgactc ccccagcgct attacccgga tgaggtttcc ggtatgcaca gccttaacag
     3541 cacccatgtc catggcagtt ccgtgccggt ggaggagccg tccacagcat caggacccgg
     3601 gcagccggcg ctcgctgtaa aaaaggataa ctaaacgctt gggatgaatt taaagaccgg
     3661 gaacgatgta ccgaggagag tgggcggcca agggatgttg aggagctgat cgaagacgac
     3721 gtagatcagc gtcgtcatga ctgcggccat caaggcggca tgccgccatt tctcaggtcc
     3781 ttccagtctc atatatgcga tgacgaagat cggcaccgtc gggatcagtc cgatcaccgc
     3841 catggagcat agaaacgcaa ccatccagcc aaagaacagg aagccgcgga ccaggatgac
     3901 tcgtcccggc aggtgggcgg tcttggacgc aatatccatg tgaatcttct gcgtcactgc
     3961 cgctttcgcc ttc
//
LOCUS       DATLJT010000151         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1156137,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000151 DATLJT010000000
VERSION     DATLJT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156137"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1702)
                     /locus_tag="VM910_03305"
     CDS             complement(314..1702)
                     /locus_tag="VM910_03305"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVI61611.1"
                     /translation="MGRFQTAITPPAAPAKPSRTITATPASEREHERILSSYGGAYDD
                     PRLETLVSKTVDRLVAASDRPDQAYKVTILNSGAVNAFALPTGQLYVTRGLIALASDT
                     SELSSVLSHEMAHVLSKHAAMREDQARQAAIVARVVSDMSNDPEITAFTLAKSKLTMA
                     NFSRDQELQADEMGVGISARAHFDPYGASRFLTSMERNAELKAGRTSLDPRAQDFLSS
                     HPATPERVSRAEAAASQYSSPENAERDREDYLGAIDNIVYGEDPSEGFVRGRRFLHPK
                     LGFTFQAPEAFTLDNTAQAVIGVREGGTQAMRFDVVRVPPEQTLGDYLNSGWMESVDK
                     SATEELTINGFPAASTVAKGDQWQFKVYALRVGADVYRFIFASHQKTTESERNARETV
                     NSFRKLTLEEIQAARPLRIKIITVQPGDTVESLSHRMAGVDHPMERFRILNGLDARAQ
                     VKVRDKVKVVVD"
     gene            complement(1830..2615)
                     /locus_tag="VM910_03310"
     CDS             complement(1830..2615)
                     /locus_tag="VM910_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVI61612.1"
                     /translation="MVFSPMMSRQPRRVALAFCALLWVAASPREGFAANCAFEPQGEG
                     RVSDVIDGKSIRLTDGREIKLAGIEPVSSSRAALSAIVAGQDVTLRSQDDSPDRYGRQ
                     SAFVFLVSSEAPVQVLLLAQGEALVSVAIVDRDCFSLLMAAEAKARDAKRGIWADPTV
                     IKNAESPDDILAGIGRFAVVEGKVLSVRQAGTTTYLNFGRNFTRDFAATISKRMIPAV
                     ESAGITLKSLENRRIRIRGWVEARPGPRIEVLGAGQIEVLGGS"
     gene            2774..>2971
                     /locus_tag="VM910_03315"
     CDS             2774..>2971
                     /locus_tag="VM910_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61613.1"
                     /translation="MKRILSALAASLLVLGAPAAQAQSKPPLKLGGILDMSSLYADIT
                     GSGSETAAKMAVEDFGGEVLGR"
BASE COUNT          531 a          960 c          938 g          542 t
ORIGIN      
        1 ttcgaccgag accgaggcga tcaaggagat cgagagccag ctcgccaaga gcccgcgccg
       61 cggcgccaag gccgaggccg gcgccgaagg tgagggcgag gccgaaggcg acatcgacga
      121 tgtcgatgat gccgacagcg atgacgctgc ggtcgccgac gaggcggcct gaaagaatcc
      181 atccgaatga attcaaaagc ccggtcgaaa gaccgggctt ttcgttttcc tcttattctc
      241 ttccacaagc ggagaggagc agcggaatta gcttttcgaa ttctccgggt tcgcgctcag
      301 cgtgctcgtc gcgtcaatcc accacgacct tgaccttgtc gcgcaccttg acctgggcgc
      361 gggcgtcgag cccgttgagg atgcggaagc gctccatcgg atgatcgacc ccggccatgc
      421 ggtgtgaaag cgattccacg gtatcgccgg gctgcacggt gatgatcttg atgcgcaacg
      481 gccgcgccgc ctggatctcc tccagcgtca gcttgcggaa cgagttgacg gtctcccgcg
      541 cgttgcgctc gctttcggtg gtcttctggt ggctcgcgaa aataaagcgg taaacgtcgg
      601 cgccgacgcg cagcgcgtag accttgaact gccactgatc gccctttgcg accgtggacg
      661 ctgccgggaa gccgttaatg gtcagttctt cggttgcgga cttgtcgacg ctctccatcc
      721 agccggaatt gaggtagtcg ccgagcgtct gctcgggcgg cacgcgcacc acgtcgaagc
      781 gcatcgcctg cgtaccgcct tcgcgcacgc cgatcacggc ctgcgcggtg ttgtcgagcg
      841 tgaacgcctc cggcgcctgg aaggtaaagc ctagcttcgg atgcaggaag cgccgaccgc
      901 gtacaaagcc ttcgctcggg tcctcgccat agacgatgtt gtcgatcgct ccgagataat
      961 cctcgcggtc gcgctcggcg ttctccggcg agctatattg gctggcggcg gcttccgccc
     1021 gcgacacgcg ctcgggcgtc gccggatgcg atgacagaaa gtcctgcgcc cgcggatcga
     1081 gcgaggtgcg gcccgccttc aattcggcat tgcgttccat cgaggtcagg aaacgcgacg
     1141 cgccataagg gtcgaaatgc gcccgcgccg atatgcccac tcccatctcg tcggcctgca
     1201 actcctgatc gcgggaaaag ttcgccatgg tgagcttgct cttcgccagc gtgaaagcgg
     1261 tgatctccgg atcgttgctc atgtcggaaa cgacgcgagc gacgatcgcg gcctgccgcg
     1321 cctggtcctc gcgcatcgcc gcgtgcttcg acagcacgtg cgccatctcg tggctcagga
     1381 ccgacgacaa ttccgaggtg tcgctcgcga gcgcgatcag cccgcgcgtc acgtagagct
     1441 gacctgtcgg cagcgcgaac gcgttcactg cgccggagtt gaggatggtc accttgtagg
     1501 cctggtcggg acggtcggac gccgcgacga gacgatcgac ggtcttgctg accagcgtct
     1561 ccagccgagg atcgtcgtag gcgccgccat aggaggacag gatgcgttcg tgctcgcgct
     1621 cggaggccgg cgtcgctgtg atggtccggc tcggtttcgc aggtgctgca ggaggcgtga
     1681 tcgcggtttg aaaccggccc atgtcgccgc agccggcgag cccgatcggc gcgcagacca
     1741 acagcgtcac agcccacagg cggcgactta ttcccctttt gcgccgtccg accaacccat
     1801 ccacgtcagc tactcgtttg tccggcggtt caacttccgc ccagcacttc gatttggccc
     1861 gcccccagca cttcaattcg tgggccggga cgtgcctcga cccagccacg aatccgaatc
     1921 cgccgattct caagggactt gagggttatc ccggcagatt cgaccgccgg gatcatgcgc
     1981 ttcgaaatag tcgcagcaaa gtcccgtgta aagttccgcc cgaaattcag gtaagtcgtt
     2041 gttcccgctt gccgtaccga cagaaccttg ccctcgacca ccgcaaaccg cccgatcccg
     2101 gccaaaatat cgtccggact ttcggcgttt tttatgacag tcgggtcggc ccagatgcct
     2161 ctttttgcgt cgcgcgcctt ggcctcggcc gccatcagaa ggctgaaaca gtcgcggtcg
     2221 acaatcgcga ccgagaccaa cgcctcgcct tgggcgagca ggaggacctg caccggcgct
     2281 tcagaggaaa cgagaaagac gaaggcgctt tgccggccat agcggtcggg cgagtcgtcc
     2341 tggcttcgca aggtcacgtc ctgccccgcg acaatggccg acaacgccgc tcggctcgat
     2401 gagaccggct cgatccccgc gagcttgatc tcgcggccgt cggtcaggcg gatgctcttg
     2461 ccgtcgatga catcggagac gcggccttcg ccttgcggtt cgaaagcgca gtttgcggca
     2521 aagccctcgc gaggcgaagc cgcgacccaa agcagagcac aaaaggcaag cgccacgcgt
     2581 cgcggctgtc tggacatcat cggcgagaaa accatcgcgt ccatgtttgc acagttcggt
     2641 gcattccgct caacggaaaa cgtcggtccc ggtcatgcgc cggccgctcc cttgtcgctc
     2701 acttgcgcat gcggcatgat cgcgcgtaac ggacaaaaag cccgcgacag cgcgtgccga
     2761 cagggaggtt ttgatgaagc gaattctttc ggcgctcgcc gcgtcgcttt tggtgcttgg
     2821 cgcgccggct gcgcaggccc agagcaaacc gccgctgaaa ctcggcggca ttctggacat
     2881 gtccagcctc tatgcggata tcaccggcag cggcagcgag acggccgcaa agatggcggt
     2941 ggaggatttt ggcggcgagg tgctcggccg c
//
LOCUS       DATLJT010000152         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3223688,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000152 DATLJT010000000
VERSION     DATLJT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223688"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VM910_03320"
     CDS             <1..1396
                     /locus_tag="VM910_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVI61614.1"
                     /translation="DATQRVLENVRSIRFTLDAEIRRMTGGLQVLALTNALRSGDFES
                     FDGMATGFLDQYGKDGVILVSDRSGQILFSSLKPEAPTLPHRNNADLVERVFSNKQPQ
                     YSNLFMGAVKKKPIVTVDVPVFRGQEVAYDISFSPPLEMFQSMVEKQRPGADWTITLI
                     DGAGTVFARIPNPQDTVGKRAPASLFAETSRRDEATLVATSLEGTPLITAFSRSPSTG
                     WTVAAGISESSLAAPLWRYLAITSVIGAVLLMVGLAFAVRMATTIARGETLHDLLVEE
                     LNHRVKNTLAILQAIAVQTFRNATKAEREKFEGRLGALAEAHNLLSTRKWQGSDLQDV
                     LVRVLQPYVLNSPERVKMFGPKVPLTPRIAVVLSMIVHEIATNAAKYGALSNDNGTVA
                     IDWEVTGQSTGPMLRLIWRETGGPPVTAPVQHGFGSRLIERSARDQLGGEATVDFLPR
                     GVVYTLTCSLKDQA"
     gene            complement(1483..2217)
                     /locus_tag="VM910_03325"
     CDS             complement(1483..2217)
                     /locus_tag="VM910_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HVI61615.1"
                     /translation="MIQNVSVPAALIAGLVSFLSPCVLPLVPPYLVYLTGATIEHLEN
                     EESVAASRRAVMISAALFVLGFSTVFVLLGASASLIGGLIRAWSAQLSILAGIVIIVM
                     GLHFLGLTRISLLMREGRLPIPKPVGLWGAYVMGLAFAFGWTPCIGPILAAILSIAAA
                     EATVVKGAGLLAVYSAGLGIPFLIAAFMVEQFSSLFARMKAHLSTVERAMGVLMIVTG
                     IGFLTGAVSSVSIWLLETFPSLQNFG"
     gene            complement(2350..2613)
                     /locus_tag="VM910_03330"
     CDS             complement(2350..2613)
                     /locus_tag="VM910_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61616.1"
                     /translation="MANSDEQLLEVLATLEECRAALTAGDNCDTAELVSVAILDIRMK
                     LNRISDAELKALCDEMLSGEASSEGARGMKSVLRRRPLLRLVK"
     gene            complement(2606..>3017)
                     /locus_tag="VM910_03335"
     CDS             complement(2606..>3017)
                     /locus_tag="VM910_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVI61617.1"
                     /translation="MVGESAFFNRATPFVRWIEGFEPYRNEMVFERASPSCYSCERFA
                     ALVNHSGGGIVLAGFAGEAACLSTLIDAFHRNHKVTYLYDASASHALDDMPAAEVHRA
                     ISTISGIYAEVCEAAAWIAATRPRKLGNGKSAGG"
BASE COUNT          619 a          950 c          935 g          513 t
ORIGIN      
        1 ggacgcgacg cagcgcgtgc tggagaatgt ccgcagcatc cgtttcaccc tcgatgccga
       61 gatcaggcgg atgaccggag gcctgcaggt gctcgcgctg accaacgcgt tgcgcagtgg
      121 cgatttcgag agtttcgacg ggatggctac aggcttcctc gaccagtacg gcaaggacgg
      181 cgtcatcctg gtctcggacc gcagcggaca gatcctgttt tcctcgctca agccggaggc
      241 gccgacgctg ccccatcgga acaacgcaga tctggtcgag agggtcttct ccaacaaaca
      301 gccgcaatac tcgaacttgt tcatgggcgc agtgaagaag aagccgatcg tcaccgtcga
      361 cgtgccggtg tttcgcggtc aggaggtggc ttacgacatc tccttctcgc cgccgctcga
      421 aatgttccag agcatggtcg agaagcaacg ccccggcgcc gactggacga tcacgctgat
      481 agacggcgcc ggcaccgtgt ttgcccgcat cccgaacccg caggataccg taggcaagcg
      541 cgcccccgcc tcactgtttg ccgagacgtc gcgaagagac gaggcgacgc tggtcgcgac
      601 gtcgctcgaa ggcaccccat tgatcacggc attttcccgc tcgccgtcga ccggctggac
      661 cgtggccgcc ggaatctcgg aaagctcgct cgccgccccg ctgtggcggt atctcgcaat
      721 cacgagcgtg atcggcgccg tgctcttgat ggtgggactt gccttcgcgg tgcgaatggc
      781 cacgaccatt gcccgcggcg agacgctgca cgatctcctg gtcgaagaac tcaatcatcg
      841 cgtcaagaat acgctggcaa tcctgcaggc gatcgcggtg caaacctttc gcaatgcgac
      901 caaggcagag cgcgaaaaat tcgagggccg tctgggtgcg ctcgccgaag cgcacaatct
      961 gctaagcacg agaaagtggc agggttccga cctgcaggac gtgctggtac gcgtgctgca
     1021 gccctatgtt ttgaatagtc cggagcgggt caagatgttc ggacccaagg tcccgctcac
     1081 gccgcggatc gccgtcgtgc tgtcgatgat cgtgcacgag atcgccacca atgccgccaa
     1141 atatggcgcg ctgtcgaacg acaacgggac cgtggcgatc gattgggaag tcacgggcca
     1201 gagcacgggc ccgatgctgc gattgatctg gcgcgaaacc ggtggcccgc cagtcaccgc
     1261 tccggtgcag cacggcttcg gatcgcggct gatcgaacgg agcgcgcgag atcaacttgg
     1321 cggcgaggcg acggtggatt ttctgccgcg cggcgtcgtc tatacgttga cctgctcgct
     1381 caaggaccag gcgtgatggg tacaccggcg tcggtgtccc gattccgaag ctcgcgttgc
     1441 gaactcgata ctccacttgt cgcgcgcttt ccggatcatg ctctatccga aattctgcag
     1501 ggacgggaac gtctcgagca gccagatgct cacgctcgac accgcgccgg tgagaaagcc
     1561 tataccggtt acgatcatca acacgcccat cgcgcgctcg acggtcgaaa ggtgcgcttt
     1621 catccgcgcg aacagcgagg agaattgctc gaccatgaag gccgcgatca ggaaggggat
     1681 gcccaggccc gccgaataga cggcgagcag gccggccccc ttcacgaccg tcgcctctgc
     1741 cgccgcgatg gagaggatgg cggccaggat cgggccgatg cagggcgtcc agccgaacgc
     1801 aaaggcgagc cccatgacat aggcgcccca cagccccacg ggtttcggga tcggcaggcg
     1861 gccttcccgc atcaacagcg agatccgcgt caggccgagg aaatgcagac ccatcacgat
     1921 gatcacgatg ccggcgagga tcgagagctg cgccgaccag gcacggatca ggccgccgat
     1981 cagggatgcg ctggcgccaa gcaagacgaa tacggttgaa aagcccagca cgaacagcgc
     2041 agccgagatc atcacggcgc gcctggatgc tgcgaccgat tcttcatttt ccagatgctc
     2101 gatggtggcg ccggtgagat agacaaggta aggcgggacc agaggcagca cacagggcga
     2161 caggaagctg accagaccgg caatcagcgc cgccgggacc gaaacgtttt gaatcatcta
     2221 agccttcgcc atcctccaaa tcccccacca gccggttcgg ccggaaagca gcgcgaacct
     2281 accggatatc agggaatgcg caactaacgc gatggtgaga tggctttgat tcgcggggcc
     2341 gcgggaagct tatttgacca gtctgagcag cggccgccgg cgcagcaccg atttcatccc
     2401 gcgcgcccct tccgaagacg cctcgcccga caacatctcg tcgcacaggg ctttcagctc
     2461 ggcgtcactg atgcgattga gcttcatcct gatgtcgaga atggcgacgg atacgagttc
     2521 ggcggtatcg cagttgtccc cggccgtaag agcggcccgg cattcctcga gcgtcgccag
     2581 aacctcgagc agttgttcgt ctgaattagc caccggcgct cttcccattg cccagcttac
     2641 gcggcctggt cgcggcgatc cacgcggccg cctcgcagac ctcggcatat atcccggata
     2701 tggtggaaat tgcgcgatgc acctctgcag ccggcatgtc gtcgagcgca tggctggccg
     2761 acgcatcgta cagataggtc actttgtgat tgcgatggaa cgcgtcgatc aaggtcgaca
     2821 ggcaggcggc ttcacccgca aatcccgcaa gcacgatgcc accgccgcta tggttcacca
     2881 atgcggcaaa cctttcgcag gaatagcagg agggacttgc ccgctcgaaa accatttcgt
     2941 tgcgataggg ctcaaacccc tcgatccagc gcacgaaagg tgtggcgcga ttgaaaaaag
     3001 ccgattcgcc gaccatg
//
LOCUS       DATLJT010000153         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3683091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000153 DATLJT010000000
VERSION     DATLJT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VM910_03340"
     CDS             <1..332
                     /locus_tag="VM910_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI61618.1"
                     /translation="ALHDLISRLSRVLLLSADRCADDIQLSQEALAEMLGVRRSSVTT
                     AAMMLRDTGAIDYRRAVIKVLDREKLHEMVCECYSTIRRAIDMGFNGARAVSRRELSL
                     PQARLSR"
     gene            842..1114
                     /locus_tag="VM910_03345"
     CDS             842..1114
                     /locus_tag="VM910_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61619.1"
                     /translation="MNRSLASNTKAVSKAVKAGSRAAADSRSRVSRVKNPDRVASTAV
                     SKSRGKADKVASAKLSATPDDQFSPASAGDFFRSESPFLVPRRSPL"
     gene            complement(1171..1455)
                     /locus_tag="VM910_03350"
     CDS             complement(1171..1455)
                     /locus_tag="VM910_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61620.1"
                     /translation="MSELVQFVVLQRSEKWVVKSRELERAFGDRRKAIHCAVELANES
                     GKNGKPALVLAGSRRHEFAPIWTFGKDPYPLARSVSRRLAKVDRPARRAG"
     gene            complement(1456..1656)
                     /locus_tag="VM910_03355"
     CDS             complement(1456..1656)
                     /locus_tag="VM910_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61621.1"
                     /translation="MPSRISEKFIGAYLNEGTLPSAVRSADANVDDKGEEEKTMGRGL
                     LLWLIGIPLPIILLIWLFGGLH"
     gene            complement(1763..2029)
                     /locus_tag="VM910_03360"
     CDS             complement(1763..2029)
                     /locus_tag="VM910_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HVI61622.1"
                     /translation="MACCRRCKKPSTTESATIGVIAVPENSHRERELHGIQELPPALK
                     QELEKFFVATDELEAKTLRFLGWQGPAQAKKLVRRCEKVFKKKN"
     gene            complement(2052..2297)
                     /locus_tag="VM910_03365"
                     /pseudo
     CDS             complement(2052..2297)
                     /locus_tag="VM910_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
     gene            complement(2321..2494)
                     /locus_tag="VM910_03370"
     CDS             complement(2321..2494)
                     /locus_tag="VM910_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVI61623.1"
                     /translation="MDKDRIKGSAQQAKGKVKEVAGKATGDKKLEGEGKADKVGGKIR
                     NAVGGMKDALRGK"
BASE COUNT          503 a          774 c          759 g          529 t
ORIGIN      
        1 atgccctgca cgatctcatc agccgcctga gccgagtctt gctgctgtcg gccgacaggt
       61 gcgccgatga tatccagctc agccaagaag cgcttgccga aatgctcgga gtgcgcagat
      121 cgagcgtgac gactgccgcc atgatgctgc gcgacaccgg cgcaatcgat tatcgtcggg
      181 ctgtcatcaa ggtgctggac cgcgaaaagc tgcacgagat ggtgtgcgag tgttactcga
      241 cgattcgtcg cgccatcgac atgggattca acggcgcccg cgcagtgagc cgacgagaat
      301 tatcgttgcc gcaagcccga ctctctcgct gaaggttgat gcacgccgct cgcgcagccg
      361 cacgaggatg gcgcagaaat ctaaaccgcg gggtgcgccc ataacgcgtt aagggtattt
      421 cagcgcaagc gttaaaggta tttcagccgc cgaattcttt cggcgtaccg ctccgctggg
      481 cggacagggc ctgatccata catgccgacc aaacggcaaa aggaaaaacg acccacagga
      541 ggatgggcat gatacacctc caaaaccttc cacagttgta caacgatcat gacgcgattc
      601 ggttccaatg atgcatcgcg cctaagtcct catttgttcg ttgcagcgaa gctgctcgac
      661 gttagtcgga gacaatctca gcgacgtctc tgctgcgttc gcatgcgaat cttgtgtgcg
      721 cgaatcagcg tgcagcgaca tgcgggccgt tacgcttgtc gaccgacgtc gccggggaac
      781 tcgacccggg caggtttgtt gtcggtaggg gaggaaacgc gaggagcagt tatgaatccg
      841 aatgaacaga agcctggcca gcaacaccaa ggcggtcagc aaggcggtca aggcgggcag
      901 cagggcggcg gcggacagcc gaagccgggt cagcagggtc aagaacccgg acagggtggc
      961 cagcacggcg gtcagcaaaa gccggggcaa ggcggacaag gtggccagcg ctaagctatc
     1021 ggctaccccg gacgatcaat tttccccggc ctcggccggg gatttttttc ggtccgagag
     1081 cccctttctc gtgccccgac gctcgccttt gtagcggccg gccaccttaa agatgttcac
     1141 tgcgaatcgg gtgcgggatg tgcgctgtcc tcaaccggcg cggcgagcgg ggcggtcgac
     1201 cttggcaagc cggcgagaaa cggatcttgc cagtgggtag ggatccttgc cgaaggtcca
     1261 aatcggtgca aattcatggc ggcgggagcc ggccaatacc agcgctggct tcccgttctt
     1321 tccgctttca tttgcaagct cgacggcgca gtgaatggcc ttgcgcctat cgccgaatgc
     1381 gcgctccaac tcgcgcgact tcacgaccca cttttcgctc cgttgcagca cgacgaactg
     1441 gacgagctcg gacatctagt gaagtccacc aaatagccaa atcagaagga tgatcggaag
     1501 cgggattccg atcagccaca gcagaagacc gcggcccatg gttttctcct cttcaccttt
     1561 gtcatcaaca tttgcatcgg ctgaacgcac tgccgatggc aaagttccct cgtttagata
     1621 ggcgccgatg aatttctcac tgatcctgct aggcatcatt gcctgtcgcg gttcacccct
     1681 gggcattaaa cgaaagttgg agatgggggc tcggtacgca accgaacatt cgcgatccgc
     1741 cggatgcatc gttggtaata ccctaattct ttttcttgaa aaccttttcg caacgccgga
     1801 ccagtttttt cgcctgtgcc ggtccctgcc agccgagaaa cctcaacgtc ttggcctcca
     1861 gctcgtcggt cgcgacgaag aatttttcca gctcttgctt gagcgcgggc ggcaactctt
     1921 gaatgccgtg cagctcccgc tcgcggtgcg agttttccgg aacggcgatg acgccgatcg
     1981 ttgcggattc ggtcgtgctt ggttttttgc atcgtctgca gcacgccaat gagcttgcag
     2041 cgcagcacga gccccggcgt ggtcgccgcg tcgtgaatga ccatggcgtc gagcggatcg
     2101 ccgtcctcgg ctcgggtcga cgggatgaag ccccagtcgt agggatacga caggccgagc
     2161 atcaaagcct tggacagaac gaaagtcttg agctcggggt cgaatgtgag cttcgccctt
     2221 gctccgcgcg gtgtttcgac caccacgtgg acgagttcat ccccggcccg agtgggtagc
     2281 ctcgctagat ttggcatcgc gatccgaacg ggttaacgcc tcacttcccg cgcaacgcgt
     2341 ccttcatgcc gccaacggca ttgcggattt tcccgccgac cttgtcggct tttccttctc
     2401 cttcgagctt cttgtcgccc gtcgccttgc cggcgacctc cttgaccttg cccttggcct
     2461 gttgagccga acccttgatg cgatccttat ccattgcatg tctccggtcg ttgtgacggg
     2521 tgaacggcga tgccgccgac aaggttccgc gcgcagctac cagcc
//
LOCUS       DATLJT010000154         3311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_926322,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000154 DATLJT010000000
VERSION     DATLJT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926322"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VM910_03375"
     CDS             <1..972
                     /locus_tag="VM910_03375"
                     /inference="COORDINATES: protein motif:HMM:NF023172.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HVI61624.1"
                     /translation="WVVLTAVVVTQMSVGGSLRATIEYTIGTLGGAVYAAAIGVLVPH
                     AGAFAQGGVLALTIAPLAFVAAINPNFRVAPFSAVLVLLLSGQLGEGPIESAFTRFLE
                     VTLGGAIAIIISLLVFSQRAHSLGAETAAEILRRMAKGFPDLLAGFTQRRDVNTMREL
                     QDDLGRSVIAFQEIAAEAKRERLVALGSEPDPAPLSRTLLRLRHDFVIIGRAVTPFPN
                     DIAQRLEPLLSCIGQNVSEFLGGSLTALILRRDCPSLQPAEASLNAYGLAINALRNEG
                     LTRALSNSEVERLFALSFALEQLHNNLIDLARCVREWAGGVGAKTTA"
     gene            1213..1341
                     /locus_tag="VM910_03380"
     CDS             1213..1341
                     /locus_tag="VM910_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61625.1"
                     /translation="MLRFHNMLPALAFVAVTGTAFFAYAGPCTTQIAQVEELGALQ"
     gene            1338..2270
                     /locus_tag="VM910_03385"
     CDS             1338..2270
                     /locus_tag="VM910_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVI61626.1"
                     /translation="MKLGIIGTGNVGNAIALAAATRGSAREIVLVNRTRKVAEAVATD
                     LRYGIPLGPRIDISDDDYDALNGAGVVLVTSGVNEKTGGATDRSDPQGRLRLLEKNAA
                     IYRDIVPRIVATAPQAGLVAVTDPPDPLADIARNVAGHNRVLSTGTFLDSLRFRVHLG
                     AHFGVDPVNVEAQVIGDHGTSQVFLWSSARIAGVPLARLLQERRESLADLRAKVEKDV
                     RYANITIIEGHDASQYGIGIVSARIAEMVLHDERSVVPIGSYQREFGITLSLPSVVGR
                     SGVTMTLPPDLSAEERNGLQRSAENLRKALERVR"
     gene            complement(2336..2797)
                     /locus_tag="VM910_03390"
     CDS             complement(2336..2797)
                     /locus_tag="VM910_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI61627.1"
                     /translation="MLMCVLFPLALSAAGTSSCTAQSIVDQMVIYAPVASQVYQRNVE
                     LGEYVSPGVPLVTLVDLSDVWIHFDLREDLVKTLKIGDRFGVRIPALSDRRILVEVKL
                     IAAKGEYSSWRATRTTGDFDLRTFSIRAYPGDKVPELRPGMSAYLDWRELG"
     gene            2832..3260
                     /locus_tag="VM910_03395"
                     /pseudo
     CDS             2832..3260
                     /locus_tag="VM910_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737265.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT          652 a          952 c         1016 g          691 t
ORIGIN      
        1 tgggtggtgc tcacggctgt cgtcgtgacc caaatgagcg ttggcggatc ccttcgagcg
       61 acgattgaat acactattgg cacgcttggc ggagccgttt acgccgccgc gattggcgtg
      121 cttgttcctc atgcgggcgc atttgcgcag ggcggcgttc tcgccctcac aatcgctcca
      181 ttagcgttcg tggcagcgat caacccgaac tttcgcgttg cgccattttc agctgtgctg
      241 gtgctactgc tgtccggaca acttggcgaa ggtccgatcg agtcggcatt cactcgattt
      301 ctggaggtca cgctcggcgg cgcaatcgcg ataatcattt cgctgctggt gttctcccaa
      361 cgagctcata gcctaggagc ggagacggct gcggaaattt tgcggcgtat ggccaagggc
      421 tttcccgatt tattggccgg cttcacgcaa cggcgcgatg tcaacacaat gcgagagttg
      481 caagatgact tgggcaggtc ggtcatcgct tttcaggaga tcgcggcaga ggcgaaacgc
      541 gagcgcctgg tcgcgctggg gtctgagccc gatccagcgc cactttcgcg aaccttgctg
      601 cggctgcggc atgattttgt cattatcggc cgcgcagtaa cgccgtttcc aaatgatatc
      661 gcgcagcggc tggagccgct actcagctgc atcggacaaa atgtcagtga atttctgggc
      721 ggcagcctaa cggcgcttat attgcgccgc gattgcccct cgctccagcc ggcggaggcg
      781 tcgctgaacg cttatggctt ggcgattaac gcgcttcgca acgagggcct gacgcgcgct
      841 ctttccaaca gtgaagttga acgcttgttc gcgctgagtt tcgcgctcga gcagctccac
      901 aacaatttaa tcgatcttgc ccggtgtgtg cgggaatggg ctggaggcgt tggagcaaaa
      961 accacggcgt gagtttgtat ttgccgggat agagcgcatt tctgcctgcg ttcgttggtc
     1021 gcgattcgtc tccaaattct tgtcagcgaa gctgcaagtt aaaatcgaat ttagccagcg
     1081 tgctttgaat ttaattggcc gttcggttca tcgcgagcat tttatcaatg cgggatccgc
     1141 actctagcga gggcgacggc gccgccggac gtggtgctga tactcggccg ccgttttctg
     1201 aaggaggcaa agatgctgcg atttcataat atgctgccgg cgttggcgtt tgtcgcagtg
     1261 accggcacgg ctttttttgc ctatgcgggg ccctgcacca cgcaaatagc acaagtcgag
     1321 gaactgggag cgctgcaatg aagctcggca tcatcggcac ggggaacgtc ggaaacgcca
     1381 ttgcgcttgc ggcagctacg cgcggcagtg cgcgcgagat cgtgctcgta aaccgcactc
     1441 gaaaggtggc ggaggccgtg gcgaccgatc tgcgttatgg gataccactc ggtccgcgga
     1501 tcgatatttc cgatgacgat tacgacgcgt tgaacggtgc cggcgtggtg ctggtcacgt
     1561 ccggcgtaaa cgaaaagacc ggtggtgcaa ctgaccgcag cgatccgcaa gggcgactga
     1621 ggcttctgga aaagaatgcc gcgatctatc gggatatagt tccaagaatc gtagctactg
     1681 cgccgcaagc agggctggtc gccgttacgg atccgcctga tccacttgcc gacattgcgc
     1741 gaaacgtcgc cggccataat cgcgtgctca gcaccggcac ctttctggac agtttacggt
     1801 ttcgtgtgca tctcggcgcc cactttggtg ttgatcccgt caatgtcgag gcgcaggtta
     1861 tcggcgacca cggcacatcg caggtgttcc tgtggtcatc cgctcgtatt gccggcgtgc
     1921 ccttggcgag actcttgcag gaacgccgcg aaagcctggc agacctgcgc gcgaaggtcg
     1981 agaaagatgt tcgctacgcc aacatcacca tcatcgaagg ccacgacgcg agccagtacg
     2041 gaatcggtat cgtatcagcc cgcattgctg aaatggtgct gcacgatgag cgcagcgtgg
     2101 tcccgatcgg cagttaccaa cgagaatttg gcatcacact gtccttgccg agtgttgttg
     2161 gtcgcagcgg cgtgacgatg actttgcctc ccgatctatc tgccgaggaa aggaatggat
     2221 tacagaggag cgcagagaac ttacgcaaag cgctagagcg cgttagatag tcgtgggtcc
     2281 ggcacgaacg aataaccgat ggcgtaaccg cttggcagcg ccgattttgc cgccctcatc
     2341 cgagctcccg ccagtccaaa taagcgctca ttccgggacg cagctccggg accttgtcgc
     2401 cagggtaggc gcgtattgag aacgtccgca ggtcaaagtc tccggtggtg cgggtggccc
     2461 gccagcttga gtattcgccc ttcgccgcga tcaatttgac ctctacgagg atgcgccgat
     2521 cactgagcgc cggaatgcga acgccgaagc gatcaccgat cttgagcgtc ttgaccagat
     2581 cctcgcgcag atcgaagtgg atccacacgt cactcagatc gaccaatgtc accagtggca
     2641 cgccaggcga aacgtattca cccagttcta cgttgcgttg gtagacctgg gaagccaccg
     2701 gcgcgtagat caccatctga tcgacaattg attgggcggt gcaggaggag gttccggccg
     2761 ccgagagcgc taacgggaac agcacacaca tgagcatgag tgtcgaagaa gcgcggaaga
     2821 tcgtcgcgcc gctcgcctgg agtcagaatc tgacgacgca gccgccagcg ccgccggcgc
     2881 gacggtcgtc gacaagcagg cgctgttccg ccaagccgat atcgtcacga tccatcttat
     2941 cctgagccga cggaccgccg gactggtcgg cgccgccgag ctcgctctca tgaagccgac
     3001 ggcgtggctg atcaatacgt cgcggggccc gattgttgaa gaagcggctc tgatagaggt
     3061 gcttgagaca cgcaggatcg ccggtgccgc gatcgacgtg ttcgacgctg agcccttgcc
     3121 cgcggatcac ccgtttcgga aggtggacaa cgtgctgatg acgccgcaca tcggctacgt
     3181 cacagaagag ctctatcgca ccttctacgg cgacgccgcc gccagttatc gcggcgtggc
     3241 tcaatgccaa tgccccctaa acagagtgcc gctgggtgcg ggtcgcgcgc ctcagcctga
     3301 tgttactcat c
//
LOCUS       DATLJT010000155         3663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_926339,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000155 DATLJT010000000
VERSION     DATLJT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926339"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VM910_03400"
     CDS             complement(<1..828)
                     /locus_tag="VM910_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVI61628.1"
                     /translation="MEQSSSLRLFGRVFLVAILVLVAVWILRPLLPALAWAGVLALAT
                     WPAREWLIRKGMTTSGAAISLTLLVGILIVGPLIVVAIEMAREAVVVVRTVQEVREIG
                     LGTPSWVMQVPFLGDYVASWWRDHLADPDAAKELLGRAESLDFIHWTRSLGSEAVNRL
                     VILAFTLLTLFFAYRDSPTIANQSRIIADRLFGPSGEHLGGEAIVAIRATVNGLVLVG
                     LAEGAALGFAYAIAGVSHPVLFGFATALLATVPFGATTVVVIACITLIIQSRITAAVL
                     "
     gene            complement(910..1305)
                     /locus_tag="VM910_03405"
     CDS             complement(910..1305)
                     /locus_tag="VM910_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61629.1"
                     /translation="MPRIKEKALKAELAKHGFESVAVKRQLPAGRVELEANKLHPVHV
                     EGGESIYAPIPVSLSVELDARGGVKSIDGGTPDPAAVADATRYVKTLRDSGQLSAAGE
                     QQPATGLTHQIERDAQGRQVLRRKRFSIS"
     gene            complement(1370..2884)
                     /locus_tag="VM910_03410"
     CDS             complement(1370..2884)
                     /locus_tag="VM910_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61630.1"
                     /translation="MAKKRSKRAAKTSAAKSKRKGKARAAAVEAKPKGVINAWEQDPG
                     DGAQPSGGQVIQRPVPVLREQPFSIRIVNPASAPEARPHSPGTADFRYWTAAEALRRG
                     ADFWGALLPGTPWEVGSTLPVDLDFGIDLNAFYDREALKFFHGSAAGRTVFSGESPDV
                     VCHELGHAVLDSLKPQLFDAASIEVAAFHESFGDMSAILSALQLPSLREGVLAETGGV
                     LHRASRLSRLAEQLGWAIRQSVPSAVEADCLRNAVNTFFYRDPDTLPTMAPATALSSE
                     PHSFSRVFTGAFFEGLAGMLAMKDTKNEAALREVSQDIAVILVEGIRGASVVPTFFSQ
                     VAATMLGVAATQFSAQGYEAPLRSGFVRHGILAPSMAVAATQAPVRAAAVAASTESTT
                     LPRLQLSVAEYGLGVPSIVVYAAAEPKRLQAAGAALAVGPAPAPGQDQAAKSFFEDLL
                     RRGRLKIPRAGKPAAEVVRSAAPLTHETHTHELRREGRQMVLRRVRIDCGGCHC"
     gene            3186..>3663
                     /locus_tag="VM910_03415"
     CDS             3186..>3663
                     /locus_tag="VM910_03415"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI61631.1"
                     /translation="MAASMRSARLHEAGQPLRIDTVEIPEPRPRDVLVQVKACGIIPN
                     MNAIFSGRLWNHLPPLPASVGLDAAGVIAKVGSEVTEVAVGDRVYVNPWLSCGMCSYC
                     RAGEPMLCSAAAFQGYFGFFPHSTRQLLDYPFGGFSEYITASPQRLVHLPPRVTFDQ"
BASE COUNT          698 a         1159 c         1123 g          683 t
ORIGIN      
        1 taagaccgcc gcggtgatgc gggactgtat gatgagggtg atgcaggcga tcacaaccac
       61 cgtcgtcgct ccgaatggaa ctgttgcgag aagcgcggtc gcgaagccga acagaacggg
      121 gtggctcacg cccgctatcg cgtaggcgaa tccgagtgct gctccctccg ccaatccaac
      181 cagcacaagg ccgttgaccg ttgcacgaat tgcaacgatg gcttccccgc cgagatgctc
      241 tccagatgga ccgaagagac gatctgcgat gattcggctt tggttcgcga tcgttggact
      301 atcccgatac gcgaaaaaga gcgtcagcaa cgtaaatgcc aagatcacta aacggttgac
      361 tgcttcgctg ccgaggcttc gcgtccagtg aataaagtcg agcgattcgg ctcgtcccaa
      421 aagctccttg gccgcatccg gatccgccag gtggtcccgc caccacgatg cgacatagtc
      481 gcccaagaaa ggaacctgca tcacccaact cggcgtgccc agtccaattt ccctgacctc
      541 ctgcaccgtg cggacaacga cgacggcttc cctcgccatc tcgatggcta caacgatgag
      601 cggaccgacg atcagaatac ccacgagaag cgtgagtgaa atcgcggcgc ccgatgtcgt
      661 catgcctttg cggatcaacc actcgcgagc gggccaggtt gcaagggcga gcacgccggc
      721 ccatgcaagt gcgggcaaga gcggccgcaa aatccaaacc gcgaccagca ccaatatcgc
      781 cacaagaaag acgcggccga acaaccgcag agatgatgac tgttccatga aaggccccca
      841 cgtcgatccc tcacatctcg tcaggaaagc tcttggaagt aaaagctcgc ctctgggccg
      901 gccatggccc tagctgatcg agaagcgttt gcggcgcagg acttgccggc cttgcgcgtc
      961 acgctcgatc tggtgagtta gcccggtagc cggctgttgt tctccagcgg cggacagctg
     1021 cccgctgtcg cgcagcgtct tgacgtagcg cgtggcatcc gcgaccgccg ccggatcggg
     1081 cgtgccgccg tcgatcgact tgacaccgcc gcgcgcgtca agctcgacgg atagcgacac
     1141 cggaatgggc gcatagatcg attcgccccc ctcgacatgc acggggtgga gcttgttggc
     1201 ttcgagctcg acgcgtccag ccggtaattg acgcttaacc gcgaccgact cgaagccgtg
     1261 ttttgcaagc tccgccttca gcgccttttc cttgatgcgc ggcattggcc agccattttt
     1321 tgacgcccga attcccctcg cgcatcatat aggccctgag cgtgcgttct caacagtggc
     1381 aacccccgca gtcgatgcga acccggcgca gcaccatctg ccgtccttcg cgccgcagtt
     1441 catgggtatg ggtttcgtgg gtgagcggcg cggcggagcg tacgacttcg gcggctggct
     1501 tgccggctct cgggatcttg agtcgccccc gtcgcagcag atcttcgaag aaggatttgg
     1561 cggcttgatc ctggcccggc gcgggtgccg gcccgacggc aagcgccgcg ccagcagcct
     1621 gcaggcgctt cggctctgcc gcggcataga ccacaatgga aggaacaccg aggccatact
     1681 cggccaccga aagttgcagc ctcggtaacg tcgttgattc cgtgcttgcg gcgacggccg
     1741 ccgcgcgcac cggcgcctgc gtggcggcca ctgccatcga tggcgccaag ataccgtggc
     1801 gaacgaaccc gctccggagc ggcgcttcat agccttgagc cgagaattgg gtggcggcaa
     1861 caccgagcat cgtcgcggca acctggctga aaaaggtggg aacaactgac gcgccgcgga
     1921 tgccttcgac caggattaca gcgatatcct ggctgacttc ccgcagcgcg gcctcgttct
     1981 tcgtatcctt catggccagc atgccggcca gtccttcgaa gaacgcgccg gtgaagacgc
     2041 gcgagaagga gtgcggctcc gaactgagcg cggtcgccgg agccatggtc ggaagcgtgt
     2101 cggggtcgcg gtagaagaaa gtattcaccg cattgcgcaa gcagtcggcc tcgaccgcgg
     2161 aaggaaccga ttgcctgatg gcccagccga gctgttccgc caaccgtgac agccgcgaag
     2221 cgcggtgaag gacaccgccg gtctccgcca acaccccctc gcgcaggctc ggtagctgaa
     2281 gcgccgatag aatcgcgctc atgtcgccga acgattcgtg gaaggcggca acctctatgc
     2341 tcgcggcgtc gaacaattgc ggcttgagcg agtcgagaac ggcgtgcccg agctcgtggc
     2401 acacgacgtc cggactctcg ccggaaaaaa ccgttcttcc cgcggccgaa ccatggaaga
     2461 acttcaaggc ctcgcggtcg taaaaggcgt tgaggtcgat cccgaaatcg agatccacgg
     2521 gcagtgtgga gcccacttcc cacggcgttc ccggcagcaa cgctccccag aaatcggcgc
     2581 cgcggcgcaa ggcttcggcg gcagtccagt agcgaaaatc cgcggttcca ggcgaatgcg
     2641 gtctagcctc gggagcagaa gccggattga cgattcggat cgagaagggc tgctctctca
     2701 gaacggggac cggcctttgg atcacctggc ctccgctcgg ctgcgcaccg tcgccgggat
     2761 cttgctccca agcattgatc acgcccttcg gcttggcttc cacggctgcc gcgcgtgcct
     2821 tgcctttgcg cttgcttttc gcggccgacg tcttcgccgc gcgtttcgat cgcttctttg
     2881 ccatcgcatc tctcccattg ctgagttacc gacatcaaat ccatgagcga aggccgcact
     2941 ggccatgatg ggcggccttt ccatcatggt gcattcagta gcgatacgac gtgagataaa
     3001 tcacagtagc tcagctgaac tctgagagtc gacgcctgct aatagccgtt ggcaatggcg
     3061 acgatgccga acgcgttttt gggaaaagtc gccccatggt cgacggaaaa tccccgggct
     3121 tcggatttcg ctgtcctttt cacggtcaac cgatctagtt tttggcgggt gacaggagga
     3181 aatcgatggc cgcttccatg cgctctgcgc gccttcatga ggctggacag ccgttgcgga
     3241 tcgatacggt ggaaattcca gagccacgtc cgcgcgatgt gctggttcag gtgaaggcgt
     3301 gcggcatcat tcccaacatg aacgcgatct tcagcggccg gctgtggaat cacctgccgc
     3361 cgctgcccgc gagcgttggg ctcgacgccg ccggcgtgat cgcgaaggtc ggaagcgagg
     3421 tcaccgaagt ggccgtgggc gaccgcgttt acgtcaatcc gtggctgtcc tgcggaatgt
     3481 gctcgtattg ccgcgcgggc gagccgatgc tgtgcagcgc cgccgctttc cagggctatt
     3541 tcggcttttt tcctcattcg acgcgtcagc tcctggatta tccgtttggc ggtttttcgg
     3601 agtacatcac tgcatccccg cagcggctgg tgcatttgcc gccgcgggtg acattcgacc
     3661 agg
//
LOCUS       DATLJT010000156         6339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1845237,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000156 DATLJT010000000
VERSION     DATLJT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845237"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VM910_03420"
     CDS             <1..309
                     /locus_tag="VM910_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVI61632.1"
                     /translation="NDADALRAARAFERAAKISFDLARQQVETGNANILLLLTAQSTY
                     LQALIQVVQARAARLADTAALFQALGGGWWNRVEPPKEKILNVGTGESFTLIDKNKFY
                     "
     gene            366..1157
                     /locus_tag="VM910_03425"
     CDS             366..1157
                     /locus_tag="VM910_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HVI61633.1"
                     /translation="MRKFPIVVAVLIAFATAGPALAAEVERVVTPDIFTAPPAFPAPR
                     VYNPPAGYEPAVIPDVFTAPVAFPAVRLYNWTGAYVGINGGGAWGRPDWTSVPDLSSG
                     TYNLSGGLVGGTLGYNLQTGEPFVWGVEADLAWSGVRGTVPPMSCVPSCEMRSDWLAT
                     ARLRFGYAFDTIMPYVTAGAAISRLVASTAGAPLGTDRANNLGWTLGLGVEFVIWGPW
                     TAKAEYLYADFNGFTCNVACGGGPISINARENIVRAGINYRIWQR"
     gene            1273..1788
                     /locus_tag="VM910_03430"
     CDS             1273..1788
                     /locus_tag="VM910_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61634.1"
                     /translation="MRVRAVWTIAFLAIAGTAVAANSWKSFRFDDDRFAIDFPDAPVT
                     TNQGFNAGTMVRTRQYYVGIKSYTFIVSATLFRYEAREEIGGDERLLRLLIDIFVAHC
                     ASHGERPVLIRGAAAREVHAENCERGPATMARYYLVDDRLYQLIVVGPTGMGTSIETQ
                     HFMRSFRIIGK"
     gene            1971..2360
                     /locus_tag="VM910_03435"
     CDS             1971..2360
                     /locus_tag="VM910_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI61635.1"
                     /translation="MHWYRSIFNHVPSTKVREIAAMLKAIHAGEDLAAAREKANRVIE
                     KLRGLRLSRAAELAETAVEATLTYYAFPEEHWRRIRTNNPLERILRDPTGALVSWARS
                     LTVNPRSTSPPQGCATSPSRRGRPRDI"
     gene            complement(2866..3150)
                     /locus_tag="VM910_03440"
     CDS             complement(2866..3150)
                     /locus_tag="VM910_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61636.1"
                     /translation="MAKYIPSPRDWVREQVELYENSGGTKDTAERLRRVFRRLGAYPD
                     WLVAKIHHAEFGSPAMNICCPDVRALMNVIGKECLHDCETDLSAAFNEAS"
     gene            complement(3234..4049)
                     /locus_tag="VM910_03445"
     CDS             complement(3234..4049)
                     /locus_tag="VM910_03445"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVI61637.1"
                     /translation="MEPDETGAAEGRQTPSQPISTDTICSALAAAAAQNDLPTDFFAR
                     LIWQESRFDPAAVSRAGAQGVAQFMPATANWRGLSNPFDPLEAIAESAKLLRDLRREF
                     GNLGLAAAAYNAGSGRVRDWLAGRRALPRETSAYVRIVTGYSPEQWAGASAGIGEMHS
                     EKPVPCSEIAAFVARTSLPASKPTPVWGVELVGGPTDETALVAYRRVEQKYASILVGR
                     QAYVVHHGLGRGSMGWAHVHVGADDRPSAEKLCADLRAAGVSYCEVQRARSTR"
     gene            complement(4141..4323)
                     /locus_tag="VM910_03450"
     CDS             complement(4141..4323)
                     /locus_tag="VM910_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61638.1"
                     /translation="MTVTIGRRELLVALGSAAAWPLAARAQQQATPVARFLTGLGIER
                     CEGRGNYSCAQRPLTR"
     gene            complement(4452..4820)
                     /locus_tag="VM910_03455"
     CDS             complement(4452..4820)
                     /locus_tag="VM910_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61639.1"
                     /translation="MARIRHLAILTEDVDRLVKFYTNSFGLKIVQGVGTATYLTDGHV
                     NLAIIPIGPEREIEGNQLKEGINHFGFEVESVEALVPVCKQWGAATDVKKRPPNREAE
                     SRVWDPDGNPIDLSQHGWPH"
     gene            complement(5030..6007)
                     /locus_tag="VM910_03460"
     CDS             complement(5030..6007)
                     /locus_tag="VM910_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61640.1"
                     /translation="MKRRTFLYLSAATAALRIASSTAWAQAYPARPVRVIVPFAPGGQ
                     VDVVARLIAQRLSARLGQQFYVENASGGGSTIGTGRATQAAPDGYTILVTDGIAFTAN
                     PSLYGKVPYDATRDFDPVAAPATTMQVLAVNPSIAARSIQELVALIKANPGKYSYASA
                     GVGTGAHLTGELFRISLGLDLVHVPYNSGGQAIAAAVGGHTPISFGSPAATIPQVNDG
                     KLRALAVGGKKRVRELPDIPTMREAGFADVECDTSILVLVPAGMPRDIIALLHDEIAR
                     AAALPDVEARLVGLGFEPFTATPDEVAALIKRELPKWAGVIRAAGIKPN"
     gene            6131..>6339
                     /locus_tag="VM910_03465"
     CDS             6131..>6339
                     /locus_tag="VM910_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61641.1"
                     /translation="MTRPRSFCDRLLRNPTTGTTACCPRRERPRGRAAEQRDELASPH
                     SITSSARVSRVVGTLRPMPLLGGRH"
BASE COUNT         1229 a         1964 c         1909 g         1237 t
ORIGIN      
        1 aacgatgccg atgcgctgag agcggcgcgc gccttcgaac gcgccgccaa gatcagcttc
       61 gacctcgcgc gccagcaggt ggagaccggc aatgccaaca tcctgctgct gctcaccgcg
      121 cagtcgacct atctgcaagc gttgatccag gtggtccagg cgcgtgccgc ccgcttggcc
      181 gacaccgcgg cgctgttcca ggcgctcggc ggcggctggt ggaaccgggt cgagccgccg
      241 aaagaaaaaa ttctcaacgt tggcacaggg gaatcgttca ctttgatcga caagaacaag
      301 ttctactgaa ggcggtgtga cgacgagggc tccgatctat ctcacctagt cactgggggt
      361 gtgtcatgag aaagttccca attgtagttg ccgtgctgat agcgttcgct actgccggcc
      421 cagccttggc ggccgaggtc gaacgcgttg tcactcctga catatttacc gcgccgccgg
      481 cttttcccgc gcccagagtc tataatccgc ccgccggcta tgaacccgca gtgattcccg
      541 atgtatttac ggcgccggtg gccttccccg ccgtaagact ctataactgg accggcgcct
      601 atgtcggcat caacggcggc ggcgcctggg gtcggccaga ttggacctcg gtccctgacc
      661 tcagcagcgg cacttacaat ctctccggcg gcctcgtcgg cggcacactc ggctacaatc
      721 tgcaaaccgg cgaacctttc gtctggggcg tggaagcaga tctcgcctgg tccggcgtca
      781 gggggaccgt cccccccatg tcctgcgtcc cgagttgcga aatgcgcagc gattggcttg
      841 cgactgcccg cctgcgattc ggatatgcct tcgataccat catgccttat gtgaccgccg
      901 gcgcggcgat cagtcgcctc gtcgcaagca ccgcgggcgc gcccttgggg acggatcgcg
      961 caaacaacct cggttggacc ttgggtctcg gcgttgagtt cgtcatctgg ggtccatgga
     1021 cggcgaaggc ggagtacctc tacgccgact tcaacggctt cacctgcaac gtggcctgcg
     1081 gcggcggtcc catctccatc aatgcacggg agaacatcgt tcgcgcaggg atcaattata
     1141 ggatttggca gaggtgaagc cttctgtgag cgtcaaaatc cttgtcggcg cgggcaacct
     1201 cgtccccgta gaacagacat aggggtacgc aagccaggaa gaaatcatct ggattagcat
     1261 cgctggtgtc agatgagagt acgtgcagtt tggacgatcg cctttttagc aattgccggc
     1321 actgcagttg ctgccaatag ttggaaatcg tttcgatttg acgatgaccg gtttgcaatt
     1381 gattttcccg acgctccagt caccacaaac caaggcttta atgcaggcac aatggtccgg
     1441 acgaggcaat actacgtcgg gatcaaatca tacactttta ttgtcagtgc gacgttattt
     1501 cgatacgagg cgcgcgagga aattggaggc gatgagaggt tgttgcgact gctcatcgat
     1561 atttttgttg cccattgtgc tagccacggt gagcgtccag ttcttattcg aggtgcagct
     1621 gcccgcgagg tacacgccga aaactgtgaa agaggtcccg caaccatggc gcgatactac
     1681 ttagttgacg atcggctcta tcagctgatc gtcgttgggc cgacggggat gggaaccagt
     1741 atagaaacgc agcacttcat gcggtctttt agaataatcg ggaagtagtg catttccaat
     1801 tcggctcagc attgcgccaa attagatgcc tgacccgctc tggaagagca aaagtgtcga
     1861 gggatgaacg cgcaactcgc cattccaact gctgatgcat cattttcggt cgcgaagaag
     1921 ctcttggcgc tgcagagttc ttgctagaag ctgcctggca aaggtctgtc gtgcactggt
     1981 atcgcagcat cttcaaccac gtgccctcga ccaaggtacg tgagatcgcg gccatgctca
     2041 aggcgatcca cgccggcgag gacctcgcgg cagcacgcga gaaggccaac cgagtgatcg
     2101 agaagctgcg tggcctgcgc ctgagccgcg ccgccgagct ggcggaaacg gcggtcgagg
     2161 caacactcac ctactacgcc ttcccggagg agcactggcg gcgaattcgc accaacaacc
     2221 cgctcgagcg catcctgcgc gatccgaccg gcgcactcgt gtcgtgggcg cgttccctga
     2281 cggtcaatcc gcgctcaacc tcgccgccgc aaggctgcgc cacatcgccg tcacggcgtg
     2341 gtcgaccaag agatatctga acatcgattg aaggaccagc acatgagagg tgccatcacc
     2401 acgtgagtgc cgggccgctc agccaactga attttccttc cgagcccctt aaaacactcg
     2461 actaattagc gtgaccggta aggaagcgac aatgatttgg cgacctcggg tttcttcgaa
     2521 ctcttaccgc agtcaaatgt caatgaaggt aacggagggg agcgggtcag ttgagaagaa
     2581 cacaggcatg agtaaggcct gtgtaaggta ttcatgcaaa atggcggggc ggcttttgaa
     2641 gagtgtgcca tgtccgtgac cgccttgagg tccctggagg cttaatccac ttgcacctcc
     2701 agggccgctg ggcagcaagt catcccatgt ctatggggaa ggtgcttgag gcgctccaat
     2761 ctattgcgga cgggatcaca atactattgg tcgccgtcgg caccgcgatc gtgttcgtga
     2821 tcatctttcg aaagttgttc cattccctca aagccatgtt ccgcgctaag aggcctcatt
     2881 aaaggccgcg ctgaggtcgg tctcgcagtc gtgcagacat tctttgccga tgacgttcat
     2941 gagagccctg acatcggggc agcagatgtt cattgcgggc gatccgaatt cggcatggtg
     3001 aatcttggcg acaagccaat ccgggtatgc acccaatcgc cggaacacgc gacgtaatcg
     3061 ttccgcggta tctttggtgc cgccgctatt ttcgtatagc tctacctgct cacgcaccca
     3121 gtctcgaggg ctcggaatgt acttagccat gggcactctc cctccgaagt gggcaatcta
     3181 tattacggct taccaaatat gtcgcgagtg gcctgggcaa gtcaggcgca ttctcaacga
     3241 gtcgatctag cgcgttgaac ctcgcagtag cttacgccag cggcacgcag atcggcacat
     3301 aatttctccg cgctggggcg gtcgtcagcc ccgacatgca cgtgggccca acccatgctg
     3361 ccgcgcccca gtccgtgatg aacgacgtag gcctggcgac cgaccaggat ggatgcgtac
     3421 ttctgctcca ctcgccgata ggcgacgagc gcagtctcgt cagtcgggcc ccccaccaat
     3481 tctacgcccc agacaggcgt cggcttgctt gcgggcaagg aggtgcgggc gacgaacgcg
     3541 gcaatctcgc tacatggaac gggcttttct gagtgcatct cgccgatgcc tgcgcttgcg
     3601 ccggcccatt gctccggcga gtagccggtt acaatgcgca cataggcgct tgtctccctc
     3661 gggagggcgc gcctgccggc gagccagtcg cgcactcgcc cggagcctgc attgtaggcg
     3721 gccgctgcaa gcccgagatt tccaaactcg cgacgcagat cgcgcaagag ctttgcggat
     3781 tcggcgatgg cttcgagcgg gtcaaaggga ttggaaagac cgcgccagtt cgcggtagca
     3841 ggcatgaact gtgccacgcc ctgcgccccg gctcggctta cagccgccgg atcgaaccgg
     3901 ctctcctgcc agatcagccg cgcgaagaaa tcggtcggca gatcgttttg cgctgcggcc
     3961 gccgcaagcg cgctgcaaat ggtatcggtc gatattggct gtgacggcgt ctggcggcct
     4021 tccgccgcgc cggtctcgtc gggttccact gcactcgcgc gctccgtgag gccgacagca
     4081 ccaaagaggg aaaccaacag ggccagaacg actgcagatc cggcaaggct gcgcatgttc
     4141 ctacctggtc agaggccgtt gcgcgcacga ataattaccg cgcccctcgc accgttcaat
     4201 tcccagtccg gtcaggaagc gggcgactgg tgttgcttgc tgctgggcgc gagccgcaag
     4261 cggccatgcc gccgcgctgc cgagtgcgac cagcaattcc cgccgtccga tggtgactgt
     4321 catacgcccc tcccgtgcga ggtgcgtaaa gggaaggata ccacgccacg agtgtgctgt
     4381 cctaacagcg cggcatccag ggcgggccgg ggcgcgcctc acggcagttc agctgatgca
     4441 actcaactca ctcagtgcgg ccatccgtgc tgcgaaaggt cgatcgggtt gccgtcggga
     4501 tcccagacgc gggattccgc ttcgcggttg ggcggtcttt tcttgacgtc ggtcgcggcg
     4561 ccccactgct tgcacaccgg aaccagggcc tcaacgctct ccacctcgaa accaaaatgg
     4621 ttgatcccct ccttgagctg attgccttcg atttcgcgtt cggggccgat cgggatgatc
     4681 gccaggttga cgtgaccgtc cgtcagatag gtagcggtcc ccacgccctg gacgatcttg
     4741 aggccgaacg aattggtgta gaatttcacc aatcgatcca cgtcttccgt gaggatggcg
     4801 agatggcgaa tgcgggccat tgagtacctc cggcgttgac atcgtcctgc cgcgcgcagc
     4861 ttacagcgga tctcgctact ttggcagctc aaggctgtgg ccaaagtcca tgtccgccgt
     4921 gcgcgaccgt gctcgcccgg cagacgaaat catcgaatga gggaggccaa gtcgcgcacg
     4981 acagcggcca agcagggcga tggcgttcat tgcgagacca aacgcaatct caattcggct
     5041 tgatgcccgc cgcgcggatg acgccagccc atttcggaag ctcgcgcttg atcagcgccg
     5101 caacctcgtc cggggtcgcc gtgaatggtt caaagccgag cccaaccagc cgcgcctcca
     5161 cgtccggcaa tgcagcggcg cgggcgatct cgtcgtgaag cagcgcaatg atatcccttg
     5221 gcattccggc cggcaccaga accaggatcg aggtatcaca ttccacgtct gcaaaacccg
     5281 cctcgcgcat ggtcgggatg tccggcagct cgcgcacgcg cttcttgccg ccgacggcga
     5341 gagcgcgcag cttgccatcg ttcacttgcg gaatggtcgc ggccggcgat ccgaaagaaa
     5401 tgggcgtatg acctccgacg gcggccgcga ttgcttgtcc tccgctattg taaggcacat
     5461 gcacgagatc gaggccgagc gagatgcgga acagctcacc cgtcaggtgc gcgcccgttc
     5521 cgacgccggc cgaagcgtag ctgtatttgc ccgggtttgc tttaatcagt gcgaccagct
     5581 cctgaattga gcgcgccgca atcgaggggt tcaccgcgag gacctgcatc gtggtcgccg
     5641 gagccgccac cggatcgaag tcgcgcgtgg catcgtacgg taccttgccg tagaggctgg
     5701 ggtttgcagt gaaagcgatt ccatccgtca cgaggatcgt atacccgtcg ggcgctgcct
     5761 gagttgcccg accggtgccg atcgtactac ctccgccgga ggcattctcg acatagaact
     5821 gttggccgag ccgcgcagac aaccgctgtg cgatcaggcg cgcgacaaca tccacctgcc
     5881 cgccgggcgc gaacggaacg atcacccgca ccggccgcgc cggataggct tgcgcccatg
     5941 ctgtcgacga agcgatgcgc agggccgcgg tggcggcact cagatataga aaggttctgc
     6001 gtttcatctc gcgctctccc gcctgccagc gccggcaggc tgataatcac cctccgcccg
     6061 gcggtattca atcgcaatgc ttcgcccctc aatgaatgcc gccccgttca ggccctggag
     6121 gaatccggga atgacgagac ccagatcatt ctgcgacagg ctgctcagaa atccgaccac
     6181 cggtactacc gcctgctgcc cgcgccgcga gcggccacgc ggccgcgccg ccgagcagcg
     6241 tgatgaactc gcgtcgcctc attcgatcac ctcgtcggcg cgggtgagcc gtgtcgttgg
     6301 gaccctgaga cctatgccct tgctcggtgg tcgtcacat
//
LOCUS       DATLJT010000157         4129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4143039,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000157 DATLJT010000000
VERSION     DATLJT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143039"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VM910_03470"
     CDS             <1..190
                     /locus_tag="VM910_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HVI61642.1"
                     /translation="KVIIRAIRNIRTGAEITYNYGRDYLTNVITRRGCKCDKCRKKRA
                     DARAKARAKSRRAHDSDA"
     gene            697..1047
                     /locus_tag="VM910_03475"
                     /pseudo
     CDS             697..1047
                     /locus_tag="VM910_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            1113..2276
                     /locus_tag="VM910_03480"
     CDS             1113..2276
                     /locus_tag="VM910_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI61643.1"
                     /translation="MGARSYRDSISFNDVYARGRTVPDAEIEHAARAIDPKDVAYLLY
                     TSGSTSTPKGVQLQHYALIENMWQIGERMHVTEHDRLWLAVSLFWGLGCENALFNLLT
                     HGGCVVLQESFEPGEALRIIGEERCTLFYGTPNMAQALSEHPYRQAHDLSSLRSGGTV
                     GTPQQIKRVVDLGAREICNIYGLTETYGNCTVTDAAEPLDIRLASVGRPLPGVDLRIV
                     DPGTGNVLPQGQVGEIRVKGYVTVGYYKDAEKNRAAFDAGGYFVTGDLGFLDAEGRLY
                     FRGRIKEMIKTGGINVAPVEVEEMLMAHPAVKLACVTGVPDPQRDEVVAALIVCQPGQ
                     RVEEADLLALCRRQLAAYKIPRMMKFISEAELPLTVTGKLQKNRLAEFFTRGA"
     gene            complement(2808..3095)
                     /locus_tag="VM910_03485"
     CDS             complement(2808..3095)
                     /locus_tag="VM910_03485"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI61644.1"
                     /translation="MTRQIHLALFMDGKLDAGAFEHMSSARRWVGKIHFGVVRAPAHT
                     DPASGELDRIVDPEAGGAVNILNDGRCDRRGRFWVGSMSKTMERATKTGTS"
     gene            complement(3562..3939)
                     /locus_tag="VM910_03490"
     CDS             complement(3562..3939)
                     /locus_tag="VM910_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI61645.1"
                     /translation="MGQAQQNRRAVLVVEDDAELRRFAARLLEDGELDTIECESAEAA
                     LATMLIRGRDVAMIFADIRLPGAMDGIDLAREVRLRWPFLPVILTSGYPREGQLPLGV
                     DFMSKPWQPLNLLVAAKQALACL"
BASE COUNT          826 a         1267 c         1258 g          778 t
ORIGIN      
        1 caaggtcatc atacgcgcca tcaggaacat cagaacaggc gccgagatta cctacaatta
       61 cggccgcgat tatctgacca atgtcatcac acggcgtggc tgcaaatgcg acaagtgccg
      121 gaagaaacga gccgatgcgc gcgcgaaggc acgcgccaaa agccgacgag cccatgattc
      181 cgacgcatga ggccaatgcc gactgattcg atttggacat tccagatcgg cagcctcgtg
      241 tcggactcat gcaccgcggc aaacgacgtg cgctgttgca ccgttattcg atcacctcgt
      301 cggcgagcgc gagcagcctg tcaggaactc ggctttggcc tcggcaagct cgcggcgcag
      361 atcggcgatc tggcattgca actcgaaatt cagttgagcg agatcagtgc gcgcttgggc
      421 ataggctcgc gcataggcga gcgcgtgcga gtcggaggat aggtctacgc agcatcggat
      481 ttgcgccaga agtttttcgg cagggcgacg acggtgccgc gctccgcatc acggggactc
      541 cctcaaaaat cagtatgcgc caccgcagcg cacctgcccg gccaattggc cggcggcgtc
      601 ttgatgtgct gccagcgcgc gtcgcataat ttgcatcggc gcccggcacc gcctgtcgcc
      661 taattcagcg cggagcacat tttccgcatg gttgcgatgc cgacgagccg tactatcccc
      721 ggcctgctcg acgagttggc ggcacgtttc ccagagcgag aggcgcttgt agggggtggg
      781 caaaggtaca cctatgcggc actgcgcgag gaggtccgag cgttcgcaaa agggctgcat
      841 gcacttggag tccgaaaggg caagatggtc gcgatcctga tgggcaacaa gcccgaatgg
      901 gtcgttgctg acctcgcgat ctgctctctc ggcggtacga tggtcgcggt caatacctgg
      961 gttaccaccc gcgagctcgg ttacgtgctc gcacattccg atgcgaccgc gctgatcgca
     1021 tgcgaccgtt ttctcagata cgactacttc gcgatgctcg acgagctcgc gccacttgcg
     1081 catccacgcc acggcttgac cacattatcc acgtgggcgc gcgttcgtac cgcgactcaa
     1141 tttcgttcaa cgacgtctac gcacgcggcc gcactgtgcc ggatgccgag atcgaacacg
     1201 cggcgcgcgc catcgaccca aaagacgtcg cttatctgct ttacacatcg ggctcgacct
     1261 cgacgcctaa gggcgtgcag cttcagcatt acgcgctgat cgaaaacatg tggcaaatcg
     1321 gcgagcgcat gcacgtcacc gagcacgacc ggctctggct cgcggtatcg ctgttctggg
     1381 gactcggttg cgagaacgca ttgttcaatc tgctgactca cggcggctgc gtcgtgctgc
     1441 aggaaagctt cgaaccgggc gaggcactgc gcatcatcgg tgaggagcga tgcacgctct
     1501 tctacgggac tcctaacatg gcgcaggcgc tgagcgaaca cccctaccgg caagctcacg
     1561 atctatcgag cctgcgctcg ggcggcacgg tcggcacgcc ccagcagatc aaacgcgtcg
     1621 tcgatcttgg tgcccgcgag atctgcaaca tctacggcct aaccgaaact tatgggaact
     1681 gcaccgtcac cgacgcagcc gagccattgg acattcggct cgccagcgtt gggcgcccgc
     1741 ttcccggcgt cgacctgcgc atcgtggacc cggggactgg caatgtgcta ccgcaaggtc
     1801 aggtcggcga gattcgcgtc aagggctacg taacggtcgg ctattacaag gatgcggaaa
     1861 agaatcgcgc cgccttcgat gcgggcggct acttcgtcac cggcgatctt ggcttcctcg
     1921 atgctgaggg gcgactgtat tttcgcgggc gcatcaagga aatgatcaag actggtggca
     1981 tcaatgttgc tccagtcgag gtcgaagaga tgctgatggc gcatccggcc gtcaagctcg
     2041 catgcgttac cggcgttccc gacccgcaac gcgacgaggt agtcgctgcg ctcatcgtgt
     2101 gccaaccagg acagagagtc gaggaagccg accttctggc gctttgccgc cgccagcttg
     2161 cggcctacaa gattcctcgc atgatgaagt tcatcagcga agctgaactg ccgctgacag
     2221 tcactggcaa gctccagaag aaccgcttgg cggagttctt cacacgcggc gcatgagatc
     2281 aatcaggaga ggggctgtcg tggtcgaaat tgcatgtgtc gcgcagaccc aggacatcct
     2341 cggtgaggtt ccgcgctggc atccgatcga gcggatgctc tattggatcg acgcattcaa
     2401 accagccatt catcgtctgg accccgtgag cgcagcgctc gaaagctgga cgccgccaga
     2461 aaagctcggc tcgtttgcgc cacgcgtggg cagaggattg ttgattgccg ggcggcgcgg
     2521 cctagcccat taccgttggc gacccagatg ttgtggtcga cagcgtccaa atgtccgcgc
     2581 tcaagtcgat agaggaggcc gtctcgcttt cgctttctgt acggcacagt gaagccacgc
     2641 cggcgggcat ccagggtcgg cggcaaaaag ctggtcgact gccttagtac aaaagcaaaa
     2701 gttgagcctg gtaaatcggt gacgagctcg gcgcagccaa attgtgcgtc gtgatcgcta
     2761 ggatgatcac cgccatcgca agaaccgtcc cgaagacaaa tgcgagccta gctggttccg
     2821 gttttggtgg cgcgctccat cgtcttgctc attgagccga cccaaaatct cccgcgcctg
     2881 tcgcagcgac cgtcattgag gatgttgacc gcgccgccgg cctccggatc aacgatgcga
     2941 tcgagctctc cgcttgccgg atcggtgtgt gctggagccc ggacgacacc aaaatgtatt
     3001 ttgccgaccc accgccgggc actgctcatg tgctcgaacg caccagcgtc aagcttgccg
     3061 tccatgaaca gcgcgaggtg gatttgacgg gtcaacagtg ccgccctggc ttgttgcagc
     3121 agtagccgac aggtgtgttg ccaatgtcgc taggccggac tttagagggg cgaattggaa
     3181 gggatgtatg agccacgtca cacttcgctg gttgtccaaa aaaaaataca acgttgcggt
     3241 agagcggcca ccaccgatga ttatgcggcc ggggcaggcg gctccacgtc gcccggactg
     3301 gcctgggcca tcagtggttc catccgatgg ccggggctgg gcgcaagcct acagagaggc
     3361 gctgctggtg ggcggcaacg aacccctagc gatcgcctac gcttgcggtt ggcgaatgtc
     3421 ttccaaagcg cgctcgcata gtagattagg attgcgttcg ccggttttcg caagctcgat
     3481 gatctatcgc ttgcttgacg tcgtctaccg gtccgcaggg cgacgcagac catttcgaat
     3541 gcgacaccca gcggtccact ctcacagaca cgctagcgcc tgtttagcgg ccacaagcag
     3601 attgagcggt tgccacggct tggacataaa atcgaccccg agcggcagct ggccctcgcg
     3661 cggatatccg gacgtgagaa tcaccgggag aaagggccag cgcaacctga cctcgcgcgc
     3721 caagtcgatc ccatccatag cgcccggaag ccggatatcc gcaaaaatca tggcgacgtc
     3781 tcgcccgcgg attagcatcg tggcaagggc ggcttcggcg ctttcacact cgatggtatc
     3841 gagttcaccg tcttcaagca gcctcgccgc gaagcggcgc agttcggcgt cgtcctcgac
     3901 gaccaacacc gcccgacggt tctgttgggc ttgtcccata ttcgccccct tcccctgagt
     3961 gttcgttacg gtccccatcc aattgtcgca tgagcgccgc caggtattcc ggaaccgggg
     4021 cgcaggcggc gtcggcgaat tcggcccgca gtccggccgc aaacgcctgc aggaactcca
     4081 tcgagtcgag ggagatggcg ggcaaacggc gcatcccaat gctccgacg
//
LOCUS       DATLJT010000158         4453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1615577,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000158 DATLJT010000000
VERSION     DATLJT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615577"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VM910_03495"
     CDS             complement(<1..236)
                     /locus_tag="VM910_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI61646.1"
                     /translation="MSVIDPTGRAIINGLQGGFPLTQQPFRDAGATLGLSEGELIDGV
                     GDLIEAGQLSRFGPLWNAELLGGGVCLAAMAVPS"
     gene            complement(236..1222)
                     /locus_tag="VM910_03500"
     CDS             complement(236..1222)
                     /locus_tag="VM910_03500"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI61647.1"
                     /translation="MTPTPRELELIDRWQHDFPLVERPFEIVGRSAALGQDETIRVFR
                     RMREHNVISRIGAVVRPNTIGASTLAALQVPPERLNRVADIVGSEPFVSHNYEREHAL
                     NLWFVIAALDRKAIVATIRTIEERTGLDVIDLPMLQAYHLGLGFPLGAGRPPRRTPVN
                     FAWHYKPNPLDRNILAAIENGLPLVANPYRAVADALELEEVDVIARLEHLIASGVVTR
                     FGCVVRHDKLGYKANAMAVWDVPDKMIDAVAGTFARHPSVTLCYRRPRHRPIWPYNLF
                     CMVHAKSRSDAYAAIDDINLQADTGLIKQAVLFSKRCFKQRGAVFSDLRRAG"
     gene            complement(1219..2394)
                     /locus_tag="VM910_03505"
     CDS             complement(1219..2394)
                     /locus_tag="VM910_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HVI61648.1"
                     /translation="MRIYARLLLALLLILPAMARGDGLRGTGNLGLVVERATGSVLIV
                     DTTSRKAVGRVEGLGDLSHASAVFSPDQRYAYVFGRDGGLTKVDLLTSSIAKRIVQAG
                     NSIGGAISDDGELIAVSNYEPGGVRIFDAQTLDAVAGIPAIGVDGKASKALGLVDLPG
                     RRFAFALYDAGEIWIADLSAPETPAITKLTGIGKLPYDGNVTPDGRHYLAGLFGEDGV
                     VHVDTWQSPLHAERILNGYGRGEEALPVYKMPHLEGWAAVGDKLLLPSIGYHELIAVD
                     LRSFTEAGRVATHGQPVFAVARPDGRYVWVNFAHPLNDTIDIVDTESLKVIHRFAPGP
                     AVLHMEFTPRGNEVWVSVRDADRVDVYDAATFARKAEIPAQKPSGIFFTARATRIGQ"
     gene            complement(2391..2678)
                     /locus_tag="VM910_03510"
     CDS             complement(2391..2678)
                     /locus_tag="VM910_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI61649.1"
                     /translation="MIAATTSASAGEAAPDTARLSNLVRQDCGSCHGLTLKGGLGKPL
                     TSEYLRAWSREQLIGIVLDGVPGTPMPPWRPVISEADARWIVDRLQQGNLP"
     gene            complement(2692..3471)
                     /gene="cobA"
                     /locus_tag="VM910_03515"
     CDS             complement(2692..3471)
                     /gene="cobA"
                     /locus_tag="VM910_03515"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HVI61650.1"
                     /translation="MGAGKVYLVGAGPGDPELLTLKAVRAIGEADAVVYDRLVPQGIL
                     ATIRDGVTRINVGKRADFHPVPQAEINHILVRLARAGRIVVRLKGGDPFIFGRGSEEA
                     AELEAAGISYEIIPGITAAQGCAASARIPLTHRGLASGVRFVTGHRKANEPLDLDWDS
                     LADPDTTLVVYMGRANVEEIARNLMVRGLPADTPVLAVCQGTTEQERRICTRLEGLQG
                     SVREAAFSGPVLFIIGRVVAIAMERSELENATDAAAMSTVA"
     gene            complement(3550..>4453)
                     /locus_tag="VM910_03520"
     CDS             complement(3550..>4453)
                     /locus_tag="VM910_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HVI61651.1"
                     /translation="PQYVLMDGATLEPLKVVSTRGMTYDTQEYHPEPRVASIVASHYK
                     PEFVVNVKETGQTLLVNYEDIKNLKVTAIEAERFLHDGGFDKSGRYFLVAANARHRVA
                     IIDTKEDKLVSVINSGGQTPHPGRGANFIHPKYGPVWATSHLGSETISFIGTDPEKHK
                     EYAWKVVQTVDGQGGGSLFIKTHPKSENLYIDTPLNTDAEISSSIAVFKIKDLAKEKP
                     EYKVLPIGQWSGISEGARRVVQGEFNKDGTEIWFSVWNNKAQESAIVVVDDKTLALKT
                     VIRDKRLVTPTGKFNVYNTRNDIY"
BASE COUNT          844 a         1457 c         1323 g          829 t
ORIGIN      
        1 tgggacggca cagccatggc ggcaaggcat acgccgccgc ccagcagttc cgcgttccac
       61 agcggcccga accggctcaa ttgtccggcc tcgatcaggt cgccgacgcc gtcgatcagt
      121 tcgccttcgc taagcccgag cgtcgcgccg gcatcgcgaa acggctgctg cgtcagggga
      181 aatcctccct gcaggccgtt gatgatcgca cgccctgtcg gatcgataac ggacatcagc
      241 ctgctctcct cagatcggag aaaaccgcac cgcgctgttt gaagcaacgc tttgaaaaca
      301 gcacggcttg cttgatcaag cccgtatcgg cttgcagatt gatgtcgtcg atcgcagcat
      361 aggcgtcgct gcgcgatttc gcatgcacca tgcagaacag attgtagggc cagatcggcc
      421 ggtggcgcgg cctccgatag cacagcgtga cgctcggatg gcgcgcaaac gttccggcga
      481 cagcgtcgat catcttgtct ggcacgtccc acaccgccat cgcattggcc ttgtagccga
      541 gcttgtcgtg gcgcacgaca cagccgaagc gcgttaccac gccgctcgcg atgagatgct
      601 ccagtcgcgc gatgacatcg acttcctcca gttcgagcgc gtcggccacc gcccgatagg
      661 gattcgcaac gagcggcaac ccgttctcga tcgcggcaag gatgttccgg tccagcggat
      721 tcggcttgta gtgccaggcg aagttgaccg gcgttcgccg cggcggtcgg cctgcaccaa
      781 gcgggaagcc gaggcccaga tgataggcct gcagcatcgg aaggtcgatg acgtcgagcc
      841 cggtccgctc ctcgatggtc cggatcgtcg caacaatcgc tttacggtcg agagctgcaa
      901 tcacaaacca gagattcagg gcgtgttcgc gctcgtaatt gtgactgacg aacggttcgc
      961 tgccaacgat atcggcaacc cggttcaacc tttccggggg tacctgcagc gccgccagcg
     1021 tgctggctcc gatggtgttc ggccgaacca cggcgccaat gcgcgagatc acattgtgct
     1081 cgcgcatccg acggaaaacc cggatcgtct cgtcctgacc aagcgccgcc gagcgtccga
     1141 cgatttcgaa gggccgctcg accaacggga aatcgtgttg ccagcggtcg atcaattcaa
     1201 gttcgcgcgg ggtcggcgtc attgcccgat cctcgtcgcg cgcgcggtga aaaagattcc
     1261 cgacggcttt tgcgcgggga tttcagcctt cctggcgaat gtcgcggcat cgtagacgtc
     1321 gacgcgatcg gcatcgcgca ccgagaccca gacctcgttg ccgcgcgggg tgaactccat
     1381 gtgcagaact gccggacccg gcgcgaagcg gtggatgacc ttgaggcttt cggtatcgac
     1441 gatatcgatg gtgtcgttca gcggatgcgc gaaattgacc cagacgtagc gcccgtccgg
     1501 tcgcgccacc gcgaacaccg gttggccatg ggtcgcgacg cggccggctt cggtgaaact
     1561 gcgcaaatcc accgcgatca attcgtgata gccaatggag gggagcaaga gcttgtcacc
     1621 gaccgcagcc cagccctcga gatgcggcat cttgtaaacg ggcaacgctt cttcgccgcg
     1681 accgtagccg ttgaggatac gctcggcgtg caacggcgat tgccaggtat cgacatgcac
     1741 gacgccgtcc tcgccgaaca ggccggcgag gtaatgcctg ccgtccggcg tgacgttacc
     1801 gtcgtaaggc agcttgccga tcccggtcag cttggtaatc gccggagttt cgggcgcgga
     1861 caaatccgcg atccagattt cgccggcatc ataaagtgca aaggcaaagc gccgccccgg
     1921 aagatcgacg agaccgagcg ctttcgaggc ctttccgtcg acgccgatcg caggaatgcc
     1981 ggcgacggcg tcaagcgtct gggcatcgaa tatgcgcaca ccgcccggtt catagttcga
     2041 aacggcgatc aattcgccgt cgtcggagat cgcgccgccg atcgaatttc ccgcctgcac
     2101 gatgcgtttg gcaatagacg atgtgagcag gtccaccttg gtcaacccac catcgcgtcc
     2161 gaacacatag gcatagcgct ggtccggcga gaacaccgcc gaagcgtggg agagatcgcc
     2221 gaggccctcg acgcggccca ccgctttccg gctggtcgta tctacaataa gcaccgaacc
     2281 ggtcgcacgc tcgaccacga gcccgagatt gccggtgccg cgcaatccgt cgccgcgcgc
     2341 catcgccggc aggatcaaca gcaaggccaa taacaggcgt gcataaatcc tcatggcagg
     2401 tttccttgct gaagccggtc gacgatccag cgcgcgtccg cctcgctgat caccggacgc
     2461 catggcggca tcggcgtgcc cggcacgcca tccagcacaa tgccgatgag ttgctcgcgg
     2521 ctccaggcac gcaaatactc ggacgtcaac ggcttgccga gccccccttt cagggtgagc
     2581 ccgtgacacg agccgcagtc ctgccgcact aaattcgata gtctcgccgt atcgggcgca
     2641 gcctcgcccg ccgacgccga agtcgtcgcg gctatcacag cgaacgcgac actaggccac
     2701 ggtcgacatc gcagcggcat ccgttgcatt ttcaagctcg ctccgctcca tggcgatggc
     2761 gacgacccgg ccgataatga atagcaccgg cccgctgaaa gctgcctccc gcacgctccc
     2821 ttggagccct tcaaggcggg tgcatattcg ccgctcctgc tcggtcgttc cttgacacac
     2881 cgcaaggacc ggcgtgtccg ccggcaaccc ccgcaccatc aaattacgcg ctatctcctc
     2941 gacattcgcg cgccccatat agacaacgag tgtggtgtcg ggatcggcga ggctgtccca
     3001 gtcgagatca agcggctcgt tggccttgcg atgcccggtg acaaagcgca cgccactggc
     3061 gagcccgcga tgcgtcaagg ggatgcgcgc tgacgccgcg cagccctgcg ccgccgtgat
     3121 gcccggaatg atctcgtacg aaatgccggc cgcctcaagc tccgccgcct cctcggagcc
     3181 gcggccgaag atgaagggat caccgccctt gagccgcacc acaatgcgcc cggcgcgtgc
     3241 gagccgaacc aagatgtgat tgatctccgc ctgcggcacc ggatggaagt cagcacgctt
     3301 cccgacattg atccgcgtca ctccgtcgcg gatcgtggcc aggatgcctt gcggcaccag
     3361 acggtcgtac acgaccgcat ccgcctcgcc gatggcgcgc accgccttca gcgtgagaag
     3421 ctcgggatcg cccggacccg ccccgaccag atagaccttt ccagcaccca ttaccgcgct
     3481 ccggccgctt cattcttcag atcgagatgg tgcggaggtc tcccgcacca tctcttgagt
     3541 cgagcgacat caatagatgt cgttacgcgt gttgtagacg ttgaactttc cggtcggcgt
     3601 caccagccgc ttgtcacgga tcaccgtctt cagcgccagt gtcttgtcgt caaccacgac
     3661 gatcgcggat tcctgcgcct tgttgttcca cacggagaac cagatttcag tcccatcctt
     3721 gttgaactcg ccctgcacca cccgccgtgc gccctcggaa atcccagacc actgaccgat
     3781 cgggagaacc ttgtattcag gcttctcctt ggcgagatcc ttgatcttga acacggcaat
     3841 cgaagacgag atttcggcat cggtgttgag cggcgtgtcg atataaagat tctccgactt
     3901 cgggtgcgtc ttgatgaata gcgagccgcc gccctggcca tcgaccgtct gcacgacctt
     3961 ccaggcatac tccttgtgct tttccgggtc cgtgccgatg aaggaaatgg tctcgctgcc
     4021 gagatggctg gtggcccaga caggcccgta tttcggatga atgaagttgg ccccgcgtcc
     4081 aggatgcggc gtctgccctc cagagttgat cacgctaacc agtttgtctt ccttggtatc
     4141 gatgatcgca acgcgatgac gggcgtttgc cgcgaccagg aaatagcggc cgctcttgtc
     4201 gaagccgcca tcatgcagaa agcgctcggc ctcgatcgct gtcaccttca gattcttgat
     4261 atcctcatag ttgaccagca gggtttgccc ggtctccttg acgttgacga cgaactccgg
     4321 tttgtaatgc gaggcgacga tggatgcgac gcgcggctcg ggatgatatt cctgcgtgtc
     4381 gtaggtcatg ccgcgcgtcg agacgacctt gagcggctcg agtgtcgcgc cgtccatcag
     4441 cacatattgc ggc
//
LOCUS       DATLJT010000159         3897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_7453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000159 DATLJT010000000
VERSION     DATLJT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1030)
                     /locus_tag="VM910_03525"
     CDS             complement(92..1030)
                     /locus_tag="VM910_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI61652.1"
                     /translation="MKASIASDRSNRASKIWIPGGSFLMGSNEFYREERPARRETVKG
                     FWADPYPVTNAEFRKFVTATGYVTTCERPPDPALYPDADPSLLVPGSSVFRKPCGPVD
                     LRDSRAWWEYVPGADWQHPEGPDSTIDGRDDHPVVHVTYEDACAYAAWAGKELPIESE
                     WEFAARGGLDGATYAWGEAFAPEGRAMANTWQGRFPWENLKLDGYEGTSPVDAFPANG
                     YGLYDMIGNVWEWTASPFMPRRAENFKKSCCVPSEPGGHVARRVVKGGSHLCAPNYCL
                     RYRPAARQGEAVDTSTCHIGFRCVVRASESRVALPA"
     gene            complement(1124..2494)
                     /locus_tag="VM910_03530"
     CDS             complement(1124..2494)
                     /locus_tag="VM910_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVI61653.1"
                     /translation="MPSEKPNILFFHVDNLGMGELGCYGGGILRGADTKRTDKFATEG
                     AKLTHYVVEPQCTPTRSALMTGRYPIRSGNHTIALGGNAGGIVRWEKTIAEILSTAGY
                     ATSCLGKWHIGAEDGRWPTDHGFDEWYGPLRTYDECMWLEDPHYVPERDGYSYMHEGR
                     KGEGVKPILSQQLTLHMKKTCDLEYQKRGLDFMQRSVSAGKPFFLYFNHSLLHFPMIA
                     RDEFVGKSTNGDWGDCLLMLDHDFGVLLDKLDDLGVADNTIVVYCGDNGAEDHLAGRG
                     TGGFFDGSYFSSAEGGIRTPFLIRWPGRIKPGTESNEMIHVTDMFTTLLRFAGSEPPR
                     DRIIDGVDQSPFFLGKQKTSNRDACMVWLNEELHAVKWKDFKINFKRQQHFHDPELPL
                     GFARITHLMEDPKEREPVNQRYVRWWVMQHARRVVWEFEESKKREELIPAGSPLDFVP
                     KPYARV"
     gene            complement(2570..2773)
                     /locus_tag="VM910_03535"
     CDS             complement(2570..2773)
                     /locus_tag="VM910_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61654.1"
                     /translation="MTYVVSHMRDQIKDALPLLRRALLRWFNVAFLKAMFDNYHPEQH
                     YMRGSGPKSRSMNSKQDVSSKQT"
     gene            complement(2832..3428)
                     /locus_tag="VM910_03540"
     CDS             complement(2832..3428)
                     /locus_tag="VM910_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61655.1"
                     /translation="MTNADIIVTEGMQPWELCATCHSLDGISAMARFPKLAGQRAAYI
                     VKQVRDFRDGRRSNDGGQMQASASNIDDDDLAKTAAYFASLPPPPPDGSLETNGEEWR
                     RGAKLFQDGDAASGIASCGSCHDDVESKRPDAPLLKAQHAAYLAKQLSDWRSGERRND
                     LSQIMPAVAAKLSDRDISALATFLASQPRLHSNGAAAQ"
     gene            complement(3513..>3897)
                     /locus_tag="VM910_03545"
     CDS             complement(3513..>3897)
                     /locus_tag="VM910_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HVI61656.1"
                     /translation="IENAWVPWAPPGLKVHVAYMTIVNRGATDRVIVGAESTDYERIE
                     LHRSVIKGGVSTMQAIDEVRVPANGRVEFAPTGLHLMLIGPRRPQAVDGHVQIVLRLS
                     SGEEVDVSAVVRRRDDAGHDAHSHH"
BASE COUNT          751 a         1182 c         1146 g          818 t
ORIGIN      
        1 gagatccaac tctaccgctg agctcgtcgc cagcgtttca atggtaggcg cagaagattt
       61 gtcggccggt ttgctccggt cgattcgtgg atcaggcggg tagagcgaca cgactttcgg
      121 acgcccgcac gacgcagcga aagccaatgt ggcaggtcga ggtgtcaacc gcttcgcctt
      181 ggcgcgccgc cggccggtac cgcaggcagt agttgggcgc gcagagatgc gaaccgcctt
      241 tcaccactcg ccgagcaaca tgaccgccgg gctcgctcgg cacgcaacag gattttttga
      301 agttttcggc gcgacgcggc atgaaggggc tcgcggtcca ttcccacaca ttgccgatca
      361 tgtcgtagag cccatagcca tttgcgggaa aggcatcgac tggagaggtg ccctcgtatc
      421 catctagctt gaggttctcc cagggaaatc ggccctgcca cgtgttagcc atcgcccgcc
      481 cctccggagc aaacgcttcg ccccatgcat aggtcgcgcc gtcgagaccg ccgcgcgccg
      541 cgaattccca ttcggattcg atcggtaact ccttgccagc ccaggccgcg tatgcgcacg
      601 catcttcgta agtgacgtgg acgaccgggt ggtcatcccg cccgtcgatg gtgctgtcgg
      661 gaccctcggg atgctgccaa tccgccccgg gcacgtattc ccaccaagca cgactgtcac
      721 gcagatccac agggccgcac ggctttcgaa acaccgaaga gccaggcact aagagagagg
      781 gatcggcatc cggatacaaa gcgggatcgg gcggacgctc gcaagttgtg acgtaacccg
      841 tggcagtaac gaatttccga aactcagcgt tggtcacggg atatggatca gcccagaatc
      901 ctttcaccgt ttcccggcgc gccggccgct cttcgcgata gaactcgttt gaacccatga
      961 gaaagctgcc gccggggatc cagatcttgg aagcccgatt cgatctatca gatgcaatgc
     1021 tcgccttcat atcgccacta cgcagaggac gttgggcttt ctacgaatct ttcgatagct
     1081 gctttatcag catttggcag gcgttttccc tgtaggcacg agtctagact cgcgcgtagg
     1141 gcttgggcac gaagtcgagc ggcgatccgg ccgggatgag ctcttctctt ttcttgcttt
     1201 cttcaaattc ccagacaacc cggcgcgcat gctgcatcac ccaccaacgc acgtagcgct
     1261 ggttaacggg ctctcgttct ttcggatcct ccatgaggtg cgtaatgcgg gcaaaaccca
     1321 gcggcagttc cgggtcgtga aagtgctgct ggcgcttgaa gttgatcttg aagtctttcc
     1381 atttgacggc gtgcagttct tcgttgagcc agaccatgca cgcgtctcgg ttcgaggttt
     1441 tctgcttgcc gagaaagaac ggactctggt cgacgccgtc gatgatccgg tcgcgcggcg
     1501 gctcggaccc ggcgaatctc aacaacgtcg tgaacatatc ggtgacgtga atcatctcgt
     1561 tgctttcggt tcccggcttg atgcggccag gccagcggat caggaacggc gtgcgaatgc
     1621 ctccttccgc cgaactaaag tacgagccat cgaagaaacc gcccgttccc cgcccggcca
     1681 agtgatcctc ggctccgtta tcgccgcagt aaacaacgat ggtattgtcg gcaaccccaa
     1741 ggtcgtcgag cttatcgagc aacacgccga agtcatggtc aagcatcagc aggcaatcac
     1801 cccaatcgcc gttggtactt ttccccacga attcgtcgcg cgcgatcatg gggaagtgca
     1861 gcaatgaatg attgaaatag aggaaaaacg gcttgcccgc ggatacgctg cgctgcatga
     1921 aatctagacc gcgtttttga tattcgaggt cgcacgtctt tttcatgtgc agggtgagct
     1981 gctgagaaag gatgggcttc acaccttcgc ccttgcggcc ctcgtgcata taggagtaac
     2041 cgtcgcgctc cggaacgtag tgggggtctt cgagccacat gcattcatca taggtgcgca
     2101 gcggcccgta ccattcgtca aagccgtgat cggtcggcca gcgtccatct tccgccccga
     2161 tgtgccactt gccgaggcat gacgtggcgt acccagccgt ggacagaatt tcggcgatgg
     2221 ttttctccca tcgcacgatg ccacccgcat tgccacccaa tgcaatggtg tggttgccgg
     2281 agcggatcgg gtagcgtcct gtcatcaagg ccgaacgggt tggcgtgcat tgcggctcga
     2341 cgacatagtg cgtcagcttg gcgccttctg tggcgaactt atctgtgcgc ttggtgtcag
     2401 ctcctctcag gatcccgccg ccgtagcaac ctaactcgcc catgcccagg ttatcgacat
     2461 ggaagaagag gatgtttggt ttttcagaag gcattgattt cctccagttg atggcagttt
     2521 tgatgaggag cggcgttgtg acggacgtct atccgcaacg gcttgccact catgtttgct
     2581 tagaactcac atcctgcttg gaattcattg agcgactttt cggcccggaa ccccgcatat
     2641 agtgttgctc gggatggtaa ttatcgaaca tcgctttgag gaatgctacg ttgaaccatc
     2701 gcaataaggc acgacgcaga agcggaagag cgtctttgat ttggtcccgc atatggctga
     2761 cgacgtaggt catgacggtc ctccggctgt cccgatttat taagccggac tttaggacca
     2821 cgaaaagcaa ttcattgcgc cgccgcgccg ttcgagtgga gccgaggttg cgaggcaaga
     2881 aatgtggcaa gcgcggaaat gtcccggtcg gacagttttg cggcgaccgc cggcatgatt
     2941 tggctcaagt cgttccgccg ttcacccgac cgccaatcgc tcagctgctt ggccagatag
     3001 gctgcgtgtt gagccttcag cagcggcgcg tccggccgtt tgctctccac gtcgtcgtga
     3061 caggaaccgc agctcgcgat gccggaagcc gcgtcaccgt cctgaaagag cttggcgccc
     3121 cgacgccatt cttccccgtt cgtctcgagc gacccatcgg gagggggcgg cggaaggctt
     3181 gcaaaatagg ccgccgtctt ggcaaggtcg tcgtcgtcga tattggacgc gctggcttgc
     3241 atttggccgc catcgttgct gcggcggccg tcacgaaagt cccggacctg ctttacgata
     3301 taggccgccc gctgcccggc aagcttcggg aagcgagcca ttgcgctgat gccgtcgagg
     3361 ctatggcagg tcgcgcacag ctcccatggc tgcatgccct cagtgacgat gatatccgca
     3421 ttcgtcaaag agaccgccga cccagcggcg attaacgcca cgcacgcacg ccaagccgca
     3481 cgttctgaga tcggcctggg catcgaacgc actcaatgat ggctgtgagc gtcgtggcct
     3541 gcgtcgtcac ggcgccggac gacggcagag acgtcgacct cttctccgct gctcaggcgc
     3601 aggacgatct ggacgtggcc gtctacggct tgcggccgcc gcgggccaat cagcatgagg
     3661 tgcaagccgg tcggcgcgaa ttcgacacgt ccgttcgccg gaaccctgac ctcatcaatc
     3721 gcctgcatcg tgctcacgcc gcccttgatg accgaccggt gaagctcgat tcgctcgtag
     3781 tcggtgctct cggcgccgac gatgacccga tcggtcgcgc cgcgattaac gatcgtcata
     3841 taggcgacgt gcaccttcag cccgggcggc gcccacggca cccaggcatt ttcgatc
//
LOCUS       DATLJT010000160         3757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1156478,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000160 DATLJT010000000
VERSION     DATLJT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156478"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VM910_03550"
     CDS             complement(<1..804)
                     /locus_tag="VM910_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61657.1"
                     /translation="MQVRRNLIAPLLVIAIVAIVWISLGMVVTNSYYQLMLTLVPIWA
                     VMGLSWNLLSGYTGLISFGHAAFFGLGAYTVTIALVRYDITPWLGVPLGMIVGTVAGI
                     VIGYPTFRLRGHYFALAMLAYPMALLYVFVWLGYQEVPLPMKREEPAFYMQFSDYRMY
                     IALAVGLLVVSLLISLAVERSRFGMSLTAIKQNEAAAEAAGIDTLVWKMRAIMLSGAV
                     AAAAGGLYAVVLLVVTPDSVFGVLTSAQALTIALFGGVGILWGPLIGAVI"
     gene            complement(807..1769)
                     /locus_tag="VM910_03555"
     CDS             complement(807..1769)
                     /locus_tag="VM910_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI61658.1"
                     /translation="MTSLGNFIEIFLQALIAGVLIGAIYGLMCVGLGIIFGVMRVINF
                     AQGEFLMLGMYFTFYLVVGFNMLWFLGPIAGPYTGALLAGLALFAVAYVLHRFLISRV
                     TGTSVAGTEGEGHYAQLILTLCVALVLQNGGLILFGSTPQSVRTPLSARAWEIPLLYD
                     ANASLFLNKARAVAGVVSVLVAAALYWLINRSRLGKTLRAAADNASAAIYMGIDVDRA
                     YRIAFGIGIGITAIAGGLVATYNPFQPYIGLDFVIIMYAGVVLGGMGSILGAFWGGMT
                     IGLVQQLSTLVLPTQLQNAAIFVVFLLIVMVRPQGLFGSATERV"
     gene            2024..3160
                     /locus_tag="VM910_03560"
     CDS             2024..3160
                     /locus_tag="VM910_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI61659.1"
                     /translation="MARPGSVAVVGVGHTRYGKLPEYDAYELGLWALKEALADSGLRF
                     EDIDGLIVNRIPDYQRFCEIAGLNPSYVSTTPGQGRFSGICIETTAALIRDGLATIVA
                     LVYGNNGRSGGDRYGGASDTYGSGGGGHWFAYGMTSPGAFHALMARRHMELYGTTADQ
                     LGAVAKTFRDHAVLNPSAVMRTPFTLADYHASRYICEPLHLLDYCLINDGGVALILTS
                     GERARDLAQRPVHVRGCGMATALAPSTFPPDDFWREPMRKAAATSFRAAGASPDDISA
                     LMIYDNFSPTVLFSLEGFGYCGEGESGPFAAEGHLALGARYPANTSGGHLSESYMQGW
                     ALNLEAVLQIRGSCGARQVKDCAFAHYMAAAPVVTSIIYGRDPS"
     gene            3157..3558
                     /locus_tag="VM910_03565"
     CDS             3157..3558
                     /locus_tag="VM910_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVI61660.1"
                     /translation="MRPLPNVDGPDQPFWRALRRGEVRLQRCGNCGLSRFPAARFCPQ
                     CRSETSEWHPVAPIGIIETWCIFHQPYFDGLSVPYTVIQVRLDCGVRLFSNPVGVETG
                     SLHIGMPVEGVFEDITPDVTLLKFKPRTDYR"
     gene            3564..>3757
                     /locus_tag="VM910_03570"
     CDS             3564..>3757
                     /locus_tag="VM910_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI61661.1"
                     /translation="MVVRAPGGTEVLKIEQIPDPTPGPKDVIIKIDACGVCFHDVVTR
                     NGTLKFGVQMPCILGHEISGT"
BASE COUNT          788 a         1119 c         1179 g          671 t
ORIGIN      
        1 gatgacggcc ccgattagcg gaccccacag gatgccgacg cctccgaaca gggcaatggt
       61 cagcgcctgc gctgacgtca gtaccccgaa cacgctgtcc ggcgtcacca cgagaagaac
      121 caccgcatat agcccgcccg ccgccgccgc gaccgcaccg ctgagcatga tcgcacgcat
      181 cttccagacg agcgtgtcga tgccagcggc ttccgcggcc gcctcgttct gcttgatggc
      241 ggtaagtgac atgccgaagc gcgaccgctc gaccgcaagc gaaattaaaa gcgacacgac
      301 tagaagaccg acggcgagcg cgatgtacat ccgatagtcc gagaactgca tgtagaaggc
      361 cggttcctcg cgcttcatgg gcagtgggac ttcctggtac cccagccaca cgaacacgta
      421 gagaagggcc atcggatagg cgagcatggc gagcgcgaag taatggccgc gcaagcggaa
      481 cgtcggatag ccgatcacga tgccggcgac cgtcccgacg atcatgccca gcggaacgcc
      541 gagccacggc gtgatgtcgt acctcactag ggcgatcgtc accgtatacg cgccaagccc
      601 gaagaacgcg gcgtggccga acgagatgag gccggtgtaa ccgctgagga ggttccatga
      661 taggcccatc actgcccaga ttgggactag tgtgagcatc agctggtaat aggaattggt
      721 cacgaccatg ccgaggctga tccacactat tgctacgatc gcaatcacga gcaggggtgc
      781 gatcaggttt cggcgaacct gcatgttcag acgcgctccg tggcactgcc gaacaggccc
      841 tgcgggcgaa ccatcacgat taataggaag accacgaaga tggcggcgtt ctgcagttga
      901 gtaggcagga cgagcgttga gagctgttgg acgagcccga tcgtcattcc gccccagaac
      961 gcgccgagaa tgctgcccat gccgccgagc accacgcccg cgtacatgat gatgacgaag
     1021 tcgagaccga tgtagggctg gaacggattg taggtggcga ccaggccacc tgcgatggcg
     1081 gtgatgccga tgccgatgcc aaacgcgatg cggtaggcgc gatcaacgtc gatacccatg
     1141 tagatggcgg ccgatgcatt atcggccgct gcgcgcagcg tcttgccgag ccgcgagcga
     1201 ttgatgagcc aataaagcgc ggccgcaaca aggaccgaga caacgccggc gacagccctt
     1261 gccttgttga ggaacagcga cgcgttcgca tcataaagca gcgggatttc ccaggcgcgc
     1321 gcggacaggg gcgtgcgcac cgattgaggc gtggagccaa acaggatgag gccgccgttc
     1381 tgcagcacaa gtgccacgca gagggtgagg atgagttgcg cgtaatgccc ttcgccttcg
     1441 gttccggcga cactggtgcc ggttacgcgc gaaatcagga agcggtgaag cacgtaggcg
     1501 acggcgaaca gcgccaggcc cgcaagcaac gcgccggtat agggaccggc gattggcccc
     1561 agaaaccaaa gcatgttgaa gccgacgacg aggtaaaagg tgaagtacat gccgagcatc
     1621 aggaactcac cctgagcgaa gttgatgacg cgcatcacgc cgaagatgat tccaagtccg
     1681 acgcacatca ggccgtaaat ggccccaatc aacacgccag cgatgagggc ctggagaaaa
     1741 atttcgataa aatttccgag tgatgtcatt tcatccgctt ccggtcagac agtcgggaga
     1801 caaagcgaaa gcggtgcgca agtattccat cggcgtgatg ctgctagcgc cggaaagcgc
     1861 gccttcgaaa cgagcgaatt gcagcagcaa gggtggaacc catgagagca tgactatgcg
     1921 ggctcgacaa caatgatgac aagtcgtcat cctctccctt ggcaagcggg gtggacatga
     1981 tcgatgctct gcgtgatacc ggccctcaag gggaagcaaa acaatggccc gcccaggatc
     2041 tgtggccgtg gtcggcgtgg ggcacacacg ctacggcaag ttaccggaat acgatgccta
     2101 tgaacttggt ttgtgggcgc tgaaggaggc actggcggat tccggccttc gttttgagga
     2161 tatcgacgga ctgattgtca accgcattcc tgactatcag cgcttctgcg agatcgcggg
     2221 attgaacccg tcttacgtaa gcacaacccc cggtcaaggc cgcttctcgg gcatttgcat
     2281 cgagaccacc gccgcgctta tccgcgatgg gctggcaacg atcgtagcac tcgtctacgg
     2341 aaacaacggc cgctcgggcg gagatcgtta cggcggggcg agcgacacct acggcagcgg
     2401 cggcggtggg cactggtttg cctatggcat gacctccccc ggcgcattcc atgcgctgat
     2461 ggcccgccgc catatggagc tttacggcac gaccgcggat caactcggcg ctgtcgcaaa
     2521 gacgtttcgt gaccacgcgg tgctcaatcc gtcggccgtg atgcgcactc ccttcactct
     2581 cgccgactat cacgcgtctc gctacatttg cgagccgctg catctgctcg actattgtct
     2641 catcaacgac ggcggcgtag cgctgatcct cacttccggc gagcgagcgc gcgacctcgc
     2701 ccaacgtccc gtccacgtgc gcggttgcgg catggcgacg gctctcgcgc cctcgacctt
     2761 tccacccgat gatttctggc gcgagcccat gcgcaaggcg gcggccacga gcttccgcgc
     2821 ggccggagcg agtccggacg acatatcggc tctcatgatc tacgacaact tctcaccgac
     2881 cgtgctcttc tcgctcgagg gcttcggcta ttgcggtgag ggtgagagcg ggccgttcgc
     2941 ggcggaaggt catctcgcgc ttggtgcgcg atatcctgca aatacatccg gtggccattt
     3001 gtccgaatcc tatatgcaag gctgggcgct caatcttgaa gcggtgctgc aaattagggg
     3061 ctcgtgcggc gcgcgccagg tgaaggactg tgcgttcgcc cattatatgg cagccgcacc
     3121 ggtcgtgacg tcgatcatct atggcaggga tccatcgtga gaccgctccc gaacgtcgat
     3181 ggaccggatc agccgttctg gcgggcgttg cgccgcggcg aggtgcgcct ccagcgctgc
     3241 ggaaattgcg gcttgtcccg gtttcccgcc gcgcggttct gcccgcagtg ccggtcggag
     3301 acgtccgaat ggcatccggt cgcgccgatt ggcatcatcg agacatggtg tatttttcat
     3361 caaccctact tcgatggtct tagcgtgcca tataccgtga tccaggtgcg actcgattgc
     3421 ggcgtccggc tgttctccaa tcctgtggga gtcgagactg gctcgctcca tatcgggatg
     3481 ccggtggagg gcgtgttcga ggacatcacg ccggacgtga ccctgttgaa gttcaagccg
     3541 cggacggatt accgatgaaa gcgatggtgg tgcgcgcgcc tggcggcact gaggtcctca
     3601 agatcgaaca aattcccgat ccgacgccgg ggccaaaaga tgtgatcatt aagatcgatg
     3661 cgtgcggcgt gtgctttcac gacgtggtca cgcgcaacgg tacgctgaaa tttggcgtgc
     3721 agatgccgtg catactggga cacgagatct ccggaac
//
LOCUS       DATLJT010000161         3753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_696317,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000161 DATLJT010000000
VERSION     DATLJT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696317"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VM910_03575"
     CDS             complement(<1..968)
                     /locus_tag="VM910_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI61662.1"
                     /translation="MPSVREWLASLGLAEYADRFAEHRIDFEILRDLTDEDLKGGIGV
                     VRVGDRRKLLRAIAELAGGASTTPQLAPGSESSPRDQAERRQVTVMFVDLVGSTALST
                     DMDPEDLREIISAYQKCVAETVRHFGGFVARYVGDGVLVYFGYPQAHEDDAERAVRAG
                     LELVTAVTRLNTHAPQQIRVGVATGLVVVGYLIASDESEERVMVGETPNLAARLQSIA
                     EPNMVVIAESTRRLLGSFFQLDDLGMRSLKGIAGPVRAWAVLQASSVASRFEALHATG
                     LTALVGREEEFEFLRRCWFKATDGEGQVVLLSGEAGIGKSRLTTAF"
     gene            complement(973..>3753)
                     /locus_tag="VM910_03580"
     CDS             complement(973..>3753)
                     /locus_tag="VM910_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVI61663.1"
                     /translation="GGPIGHRVPGEHGFRFFPGFYKHVIDTMRRIPSFDGAKVADHLV
                     PATRLEFTQYDEPPFVVPAGFPASPRDAGTILRDTLLLFAPGADLTPDDLAFFGARLW
                     QILTSCKERRLGEYERASWWDFVGAEERSVGYQKFLATGITRSLVAAKARKASTRTIG
                     DIFVQLVLTILNPIAGSTDRLLDGPTNLVWIDPWLKYLESSGVEYIKNAEVEEILCEN
                     GRITGVVARQQGKRAVVRGDHYVAALPLEDIAPMVNSGLAAADPALAKLRALAVNLEW
                     MSGVQFYLHRAVPSAHGHVAHIDTEWALTSISQLQFWRNAPPEVFGDSDVHGILSVDI
                     SDWTAPGSNGRPAMQCSREEVVRETWSQLKRSLNDAGDEVLRDEDLQSWFLDPSIESD
                     PARPGWLRNIEPLLVNLVDSWALRPQATTAIPNLFLASDYVRTYTDLATMEGANEAAR
                     RAVNGLLDAVNFDGSRCELWPLHEPEVLAPWRLYDAARYQAGLPWDHSLVQVAAHAIR
                     GASPLLEQARPLLEQVAPFGHTIADTLDPRSAAAGDVGLLDIVDPTAAGRRLVDVPTL
                     QDVLIKAVDDVPAAGDIVGPAGFLDRLVWYRGMLTETLAEGVPTWEPQRHLYGLVKEF
                     IGRSGKGLRPALCIATARALGGRMEDAFPAAAGLEMLHNAFLVHDDIEDGSDLRRGVA
                     TMHRHAGIPIALNTGDSMNALAMRLFRRTGERLGPAAAMRIFDEVDHMVVETLEGQAM
                     ELGWVRDNDLTLGADDYLRLVLKKTSWYSFIHPMRIGALVANGEDRNLSRFNRFGYLL
                     GLAFQITDDVLNLKGNVGRYGKEINGDLWEGKRTLLLTRALDRAHGADRAWISGFLAR
                     PRERRLPREVLRLHEIIASSGSIKWAQQAAAAFAEAAAREFDTSAFAGVPASPDLEWL
                     RACVDYLVRRDA"
BASE COUNT          664 a         1265 c         1171 g          653 t
ORIGIN      
        1 ataaacgcgg tcgtaagtcg tgatttcccg ataccggcct cgccggagag tagcacgact
       61 tggccttcac catccgttgc cttgaaccag caccgtcgca ggaattcgaa ttcttcttcg
      121 cgcccgacga gggcggtcaa gccggtcgcg tgcagcgcct cgaagcggct tgccacggaa
      181 ctcgcctgca gcaccgccca cgcccgcacc ggcccggcaa tacccttgag gctcctcatc
      241 ccaaggtcat cgagctggaa gaaactgccg agaagtcttc gcgtgctctc ggcgatgacg
      301 accatattcg gttcggcgat gctttgcagg cgcgccgcaa ggttcggcgt ctcgcctacc
      361 atgacgcgct cctccgactc atccgatgct atgaggtagc ccaccacgac cagtccggtt
      421 gcgacaccaa cgcggatttg ctggggagcg tgcgtgttga gccgagtcac cgcggtaacc
      481 agctcaagcc cagcccgtac cgctcgctcg gcgtcgtcct catgggcctg cggatagcca
      541 aagtacacga ggacgccatc cccgacatac cgggcgacga acccgccgaa gtggcgcacg
      601 gtctcggcga cgcatttctg ataggccgaa atgatctctc gcaagtcctc cgggtccatg
      661 tcggtggaaa gggcggtcga accgaccagg tccacgaaca tcaccgtgac ttggcggcgc
      721 tcggcttggt cccgtgggct cgattcggac ccaggcgcaa gctggggcgt ggtggaggcg
      781 cctccggcaa gctcggcaat cgcccggagc aatttccgac gatcgccaac gcgcacgacg
      841 ccaatccccc ccttgagatc ctcgtccgtg aggtcccgaa ggatctcaaa atcgatccgg
      901 tgctcggcaa agcggtccgc atactcggcc aatccgagcg aagcaagcca ctctctgacc
      961 gaaggcattg tgctacgcat ctcgtcgcac gaggtagtct acgcaagcgc gcagccattc
     1021 gagatcggga cttgctggca cgccagcgaa tgccgaggtg tcgaactcgc gcgccgcagc
     1081 ttctgcgaat gcggcagccg cctgctgcgc ccacttgatg ctgccgctgc tcgcgatgat
     1141 ttcgtgaagc cgcaacactt cgcgcggtag gcggcgctcg cgcggccggg ccaggaagcc
     1201 ggaaatccac gctcggtctg cgccatgggc gcggtcgagc gcgcgcgtca gcagcaaggt
     1261 ccgtttgccc tcccagaggt caccgttgat ttccttgccg tatcgtccaa cgttcccctt
     1321 caggttcaga acgtcgtcgg tgatctggaa ggcgagcccg agcaggtagc cgaaccgatt
     1381 gaatcggctg agattccgat cctcgccatt ggcgacgagg gcgccaattc gcatcgggtg
     1441 aatgaagctg taccaagaag tcttcttgag tacaagccgc aggtagtcgt ccgccccaag
     1501 ggtgagatcg ttgtctcgta cccagccgag ctccatggcc tgcccttcca gggtctcgac
     1561 gaccatatga tcgacttcat cgaagatgcg catcgccgct gccggtccga gtcgctcgcc
     1621 ggtcctgcga aaaagccgca tggcaagcgc gttcatggaa tcgccggtgt tcagggcaat
     1681 cgggatcccc gcgtggcgat gcatcgtcgc gaccccgcgc cgcaagtcac tgccgtcttc
     1741 gatatcgtcg tgcacgagaa atgcattgtg cagcatttcc aggccggcgg cggcgggaaa
     1801 cgcatcctcc atgcggccgc ccagcgccct cgcggtcgcg atgcacaggg cagggcgaag
     1861 acccttgcct gaccggccga tgaactcctt cacgagccca tacaggtgcc gctgcggctc
     1921 ccacgtcggt acgccttctg ccagcgtctc ggtgagcatg ccgcgatacc agaccaggcg
     1981 gtccaggaac cccgccgggc ccacgatatc gccggcggct ggcacgtcgt cgacggcctt
     2041 tatcagcaca tcctgcaaag tgggcacatc aaccaagcgg cgccccgccg ccgtcgggtc
     2101 gactatgtcg agcaggccga cgtcccccgc ggcggcactg cgcgggtcga gcgtgtcagc
     2161 gatggtgtgg ccgaacggcg cgacctgttc aagcaggggg cgcgcctgct cgaggagcgg
     2221 cgacgcaccg cgtatggcgt gggcagccac ctgcacgagc gaatggtccc acggcaggcc
     2281 ggcctggtat cgcgctgcat cgtacagccg ccacggcgcg aggacttcgg gttcgtgcag
     2341 cggccatagc tcacagcggc ttccatcgaa gttcacggcg tccagcagcc cgttcaccgc
     2401 gcgccgcgct gcctcattgg ctccctccat ggtcgccagg tcggtatacg tgcgtacgta
     2461 gtccgacgct agaaacaggt tcggaatggc cgtggtcgcc tggggacgca atgcccagct
     2521 gtcgacgagg ttcaccagca gcggctcgat atttcgcaac caccccgggc gtgcgggatc
     2581 gctctcaatg gacggatcga ggaaccacga ctgcaaatcc tcgtcgcgca gcacttcgtc
     2641 accggcgtca ttgagcgagc gcttgagctg gctccatgtc tcgcgcacca cctcttcacg
     2701 cgagcactgc atggccgggc gtccattcga ccctggggcc gtccagtcgg aaatatcgac
     2761 cgagagaatg ccgtgcacgt cgctgtctcc gaacacctcg ggcggcgcgt tgcgccagaa
     2821 ctggagctgc gaaatgctcg tgagcgccca ttcggtgtcg atgtgggcga catggccgtg
     2881 cgccgacggc accgcgcggt gcagatagaa ctgcacgccg ctcatccact ccaagttcac
     2941 ggccagcgcg cgcagcttgg cgagcgccgg gtccgctgcc gccagtccgc tgttcaccat
     3001 gggggctatg tcctcgagcg gcagcgccgc cacgtagtgg tcgccacgca ccacggcgcg
     3061 cttcccctgt tggcgagcga ccacgccggt gatccgtccg ttctcgcaca ggatttcctc
     3121 gacctccgcg ttcttgatgt actccacgcc gctcgattcg agatacttga gccatggatc
     3181 gatccacacg aggttcgttg gcccgtcgag caagcggtcg gtcgatcccg cgatcggatt
     3241 gaggatcgtg agcaccagct gcacaaagat gtcaccgatg gttcgcgtgc tggccttgcg
     3301 tgccttggcg gccaccagcg agcgtgttat tcccgtcgcc aggaacttct ggtaccccac
     3361 cgaccgctcc tccgcgccga cgaagtccca ccagctcgcc cgttcgtact ccccgagccg
     3421 gcgctctttg cacgaggtga ggatctgcca gaggcgcgcg ccgaagaatg ccagatcgtc
     3481 gggggtgagg tcggcgcccg gtgcgaacag cagcagggtg tcccgcaaaa tggtgccggc
     3541 atcgcggggc gaggccggaa agccggccgg taccacgaat gggggttcgt cgtactgggt
     3601 aaattccagc cgcgtcgccg gcacgaggtg gtccgccact ttggccccat caaacgatgg
     3661 tatgcggcgc atggtgtcga tgacgtgctt gtagaagccc gggaagaacc gaaacccgtg
     3721 ctcgccgggc acccggtgcc caatcggacc tcc
//
LOCUS       DATLJT010000162         2805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1156856,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000162 DATLJT010000000
VERSION     DATLJT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156856"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VM910_03585"
     CDS             <1..838
                     /locus_tag="VM910_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61664.1"
                     /translation="RQNSTQVNFVIRGRGHSIVGGKRFEVNLYDVWNTPSMHIYWHGN
                     DSKDIQVRLTYSNAALLELMNIHYVEENPPPIEPVKHEQQSEDDKRRRISPYGTIKLD
                     DEGACLMPYEVLINPPAVESKALHWPWEQVKHHLDKLQALGKDYIGRRLYMLYNPMTG
                     RTNGCTPSFFATMTIRPPKIVDRPHRHTSAAINYYFHGTGRSTVGGEVLEWKAGDLML
                     SAPGWAVHNHASYDDFVYELTVQDQPLNIAMESLLWQESLKLPLAVLGVQEGFGTNRA
                     VG"
     gene            1019..1987
                     /locus_tag="VM910_03590"
     CDS             1019..1987
                     /locus_tag="VM910_03590"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61665.1"
                     /translation="MPIRVVMRACALALVALFTLTPARAEDQLKVAIGQINNWENQAP
                     TLGQDAGIFKRHNLVLENVGTQGAGETMQPVIAGSADLGAGVGVAGVMRAFARGAPLR
                     ILLPAFTGTGDLFWYVKTESPLKTIKNITANNTIAYSTSGSSSNNLVLAFVDELGVKA
                     KPTPTGGPAATLTQVMSGQIDIGWAAPPFGMKEIKEGKIRILIRGSDAPSLRGQTVRT
                     IIVNADALKTKRDAIMRFAQGYREAVDWMYSDPKAIAMYAEKMKLDPDLIANSIKEFQ
                     PKEALQTDEMKDMAGIQRDAVKLKFIDAPLTKEQLAELIQIPPRAK"
     gene            2039..>2805
                     /locus_tag="VM910_03595"
     CDS             2039..>2805
                     /locus_tag="VM910_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61666.1"
                     /translation="MKATMRNWRWLLSLVLAGSMAVAGSGASAQDTLKVAIGQLGLWA
                     VDAPRLGQRAGIFKKHGLVLDIFGTSGGGDTLQAVISGSADLTVGIGTAAVLRAYSKG
                     APIRVIGANFTGAGDFYWYVRADTPIKRLTDASDKTTIGYSASGSSSHNVVLAFLQEL
                     GVKATPTATGTQPATLTQVMSGQIDIGWSAPPFGLKESAEGKIRIIANGNDVPSLRSQ
                     TVRVDMVNADVLKDRRDALLRFVRAYRETLDWMFSSP"
BASE COUNT          558 a          881 c          886 g          480 t
ORIGIN      
        1 ccggcagaac tcgacccagg tcaactttgt tattcgcggc cgcggtcatt cgatcgtggg
       61 cggcaagcgc tttgaagtga atctgtacga tgtctggaat acgccctcca tgcatatcta
      121 ttggcatggc aacgacagca aggatatcca agttcgactc acctactcga acgccgcctt
      181 gctcgaactg atgaacatcc attacgtgga ggagaacccg ccgccgatcg agccggtcaa
      241 gcacgagcag caaagcgagg acgacaaacg tcgccgcatc tcgccctacg gaacgatcaa
      301 gctcgacgac gagggcgcgt gtctcatgcc ctacgaggtg ctgatcaacc cgccggcggt
      361 ggagtcgaag gcgctgcact ggccgtggga gcaggtcaaa catcatctcg acaagttgca
      421 ggcgctgggc aaggactata tcgggcggcg gctctatatg ctctacaatc cgatgaccgg
      481 gcgcaccaac ggttgcacac cgtcgttctt cgccacgatg acgatacgcc ctcccaagat
      541 cgtcgaccgg ccgcaccgcc acacctcggc ggccatcaat tactacttcc acggcaccgg
      601 acgctcgacc gtcggcggcg aggtgctcga gtggaaggcg ggcgacctca tgctgtccgc
      661 gcccggctgg gcggtgcaca accacgcctc ctacgatgat ttcgtctacg agctgacggt
      721 gcaggatcag ccgctgaaca tcgccatgga aagcctgctg tggcaggaga gcctcaagct
      781 gccgctagcg gttctcggcg tgcaggaggg cttcgggacc aatcgggcgg tgggctgagc
      841 gcaacgagcc gaagcgcggc tcgccggcgc cgcacctcgt tgctggaacg aaagcacggc
      901 cagtggcgca gtgtcgcgcg ccttgcgtgg cgtctcgcgc gatctgccga cgccatgcta
      961 cgcttgtcgc agggcgggca actctatgct gtcgctccgt ttcggaggac ctgcgatgat
     1021 gccaatacgc gttgtgatgc gggcgtgcgc gctcgcgctt gttgcgctgt tcactctcac
     1081 gccggcgcgg gcggaagatc agctcaaggt cgccatcggc cagatcaaca attgggagaa
     1141 ccaagcgccg acgctcgggc aggacgcggg catcttcaag cggcataacc tcgtgctcga
     1201 gaacgtcggc acccagggcg cgggcgagac catgcagccc gtcatcgccg gcagcgccga
     1261 tcttggcgcc ggcgtcggcg ttgccggcgt catgcgcgct ttcgccagag gcgcgccact
     1321 gcgcatcctg ctgcccgcgt tcaccgggac cggagacctg ttttggtacg tgaaaacgga
     1381 gtcgccgctc aagaccatca agaacatcac cgccaacaac acgattgcct attccaccag
     1441 cggttcgagc tcgaacaacc tcgtgctggc attcgtcgac gagctcggcg tcaaggccaa
     1501 accgacgccg accggcgggc ccgccgccac gctgacccag gtcatgtccg gtcagatcga
     1561 tatcggctgg gccgccccgc cgttcggcat gaaggagatc aaggaaggaa agatccgaat
     1621 cctcatccgc ggcagcgacg cgccttcgct gcgcgggcag acggtgcgaa ccatcatcgt
     1681 caacgccgac gcgttgaaga ccaagcgcga cgcgatcatg cgtttcgccc aaggctatcg
     1741 cgaggctgtc gattggatgt attcggatcc caaggcgatc gcgatgtatg ccgagaagat
     1801 gaagctcgat ccggacctga tcgcaaactc gatcaaggaa tttcaaccga aagaggcgtt
     1861 gcagacggac gagatgaagg acatggctgg gattcagcgg gacgcggtca agctcaaatt
     1921 catcgatgcg ccgctgacca aagaacaact tgccgaactc atccaaatcc cgccgcgcgc
     1981 gaaataagga gcaagaaaaa aggccgtatc cgggacaggc ggccttaagc taacgaccat
     2041 gaaggcgacc atgcgcaatt ggcgctggct gttgtcgctt gtgctcgcgg ggtccatggc
     2101 agttgccggc tcgggagcgt cggcgcagga cacgctcaag gtggcgatcg ggcaactcgg
     2161 gctgtgggcg gtcgatgcgc cgcggctcgg acagcgcgcc ggcatcttca agaagcatgg
     2221 cctcgtcctc gatatcttcg gcacctccgg cggcggcgac acgttgcagg ctgtgatttc
     2281 cggctccgcc gacctcacgg tcgggatcgg caccgccgcg gtcttgcgcg cctattccaa
     2341 gggcgcgccc atccgcgtga tcggggcgaa ctttaccggg gcaggggact tctattggta
     2401 cgtgcgggcg gatacgccca tcaagcgcct cacggatgcc agcgacaaga cgaccatagg
     2461 ttattcggcg agcggatcct cgagtcacaa cgtggtcctt gccttcctgc aggagctcgg
     2521 cgtcaaggcg acgccgacgg ccaccgggac gcagcctgcg acgctcactc aggtgatgtc
     2581 cgggcagatc gacatcggat ggagcgcgcc gccgttcgga ttgaaggaat cggccgaggg
     2641 taagattcgc atcatcgcca acggcaacga cgtgccctcg ctgcgcagcc agaccgtccg
     2701 cgtcgatatg gtaaatgccg acgtcctcaa ggaccgcagg gacgcgctgt tgcgtttcgt
     2761 gcgcgcttac cgcgagactt tggattggat gttttccagt ccgga
//
LOCUS       DATLJT010000163        10015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_696681,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000163 DATLJT010000000
VERSION     DATLJT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696681"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..831)
                     /locus_tag="VM910_03600"
     CDS             complement(82..831)
                     /locus_tag="VM910_03600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVI61667.1"
                     /translation="MMDPIHQFEIKNFFPIVRIGGVEIAFTNSALFMLIGLTLILLLM
                     LGATRSRALVPGRLQAAAEMSYEFVADTLRSTTGTEGMKFFPLVFSIFMFILVLNLIG
                     IIPYTFSVTSHIVITVAFALLVFFTVVIYGFWLHGLRFLKLFVPKGVPIYILPLVTAI
                     EVLSFFSRPVSHSVRLFANMLAGHITLKVFGGFVVMLGAWGVLGWLGAALPLALTAAL
                     TALELLVALLQAYVFAILTCIYLNDALHPGH"
     gene            complement(891..1268)
                     /locus_tag="VM910_03605"
     CDS             complement(891..1268)
                     /locus_tag="VM910_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVI61668.1"
                     /translation="MADNAREKGDREQPPADEAALSARFKRLGERLGRVHADRPSENA
                     TGQRPTADPSAIARGFRLSTEFVAGVVVGAAIGWLLDRWLGISPWGMIVFLLLGFAAG
                     VLNVMRAAGVVASNTLDPPGSKQ"
     gene            1605..2069
                     /locus_tag="VM910_03610"
     CDS             1605..2069
                     /locus_tag="VM910_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61669.1"
                     /translation="MIRTALFVVAGLALAVPAAWAQSTAPESDDSRYTFNRIDDGYLR
                     LDGRTGQVSICARHPVGWTCQAVPDERAALEAEIARLQGENIAIKKELLARNLPLPGT
                     VKPEPPAAKPEEPRVQLPNDADLDKVMNFIEKIWRRLVEMIATLRKDMLKKT"
     gene            2092..2553
                     /locus_tag="VM910_03615"
     CDS             2092..2553
                     /locus_tag="VM910_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVI61670.1"
                     /translation="MSDPLSPIISFAPETIATATLVVETSGQAFTEITREAMRFIKQT
                     GAKDGALFIFMRHTSGSLVIQENADPDVRTDLVSALDRLAPADAQWVHDVEGPDDMPA
                     HVKTMLTGVSLHVPVIAGALALGVWQGIYVAEHRARPHRREIVLQFIGSRQ"
     gene            2682..2861
                     /locus_tag="VM910_03620"
     CDS             2682..2861
                     /locus_tag="VM910_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61671.1"
                     /translation="MFAPMAVKTLNELMHRHALEFASLHGLRNRKSGSLGRFEDASIE
                     SVDRHSGVASLRIFT"
     gene            complement(2784..2972)
                     /locus_tag="VM910_03625"
     CDS             complement(2784..2972)
                     /locus_tag="VM910_03625"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HVI61672.1"
                     /translation="MLCLYGHPHAQVRLKAVKATLAVAPERARRMLEIMAQSREYPQA
                     GDAGMTIDALDRGIFKPT"
     gene            complement(3128..3499)
                     /locus_tag="VM910_03630"
     CDS             complement(3128..3499)
                     /locus_tag="VM910_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HVI61673.1"
                     /translation="MKRAKLQDITVSQLVERFTGIGIEQDQALLRRQYGKFNRLFDEM
                     VAIEDELKRRDGDQRRELLSLYKHRNAQVRLNAIKATLAVSPEIARQALQALADSREY
                     PQAGDAGMSLWNLEQGIFKPK"
     gene            complement(3496..4227)
                     /locus_tag="VM910_03635"
     CDS             complement(3496..4227)
                     /locus_tag="VM910_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61674.1"
                     /translation="MIVRYDQAYQFVRPLHLPRNLCSWAPLPDSIARLPDRVHDLPSV
                     GFDRVLANTDKPGLLPAADELQHDFAPVRPCAMLRELAAPPPPIIPRERPPTASEHYD
                     IARATARWLAQRWVAQEQRHVVEAPNWFLEYEPSVQAYLDPPKTLQELQQAVSTPKPG
                     YDIHHIVEKTSAEQDRFPRDMINAPENLVLISRFKHWEITGWYMTRNKDYDGLPPRDY
                     LRGKDWDERTRVGLRALIQHGVLKP"
     gene            4198..4860
                     /locus_tag="VM910_03640"
     CDS             4198..4860
                     /locus_tag="VM910_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI61675.1"
                     /translation="MVRLIIADDHPLFRGALREAVSGLFEEVEIAEAGSFDDVAKLLE
                     RGAEVDLILLDLTMPGVRGFSGLMYLRAQYPSVPIVVVSANDDPGVIRRCMDFGALGF
                     IPKTLGIEAMRGAISRVFEGGVWTPPDIDLGAGSDAETADLLTRLTSLTPQQVRVLMM
                     LSEGLLNKQIAYELSVSEATVKAHVSAILQKLGVESRTQAVIAASKIELGQWPQTVSA
                     GH"
     gene            complement(4901..8425)
                     /locus_tag="VM910_03645"
     CDS             complement(4901..8425)
                     /locus_tag="VM910_03645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HVI61676.1"
                     /translation="MLQGWIVIAVALGYIGLLFVVASYGDRTRRFGREGGWRHFIYPL
                     SLAIYCTSWTFFGSVGLASRTGFDFLTIYIGPILLVGLCSPLIMRIVRLAKAQNITSI
                     ADFIAARYGKAQTVAATVALIAIIGTIPYIALQLKAVSSSLTTILVHIGPASGAMQPV
                     LGDIALYVAVAMAIFAVLFGTRHIDATEHQDGLMLAIATESIVKLVAFVGVGIFVTFW
                     MFDGPRILFAQAMERPDTAAVLTREPAPGTLLAMTLLSLFAIILLPRQFHVTVVENNS
                     ENEIRRASWLFPLYLVLINLFVVPIALAGLLTFTPGQVDSDMFVLALPLAAHSDLFTI
                     AAFVGGLSAATAMVIVESVALAIMVSNDIVMPLVLQRREALITGRDNIGGLLLTVRRM
                     AIFVILFLAYVYYRLAGDAQLASIGLLSFAAVAQLAPAFFGGLIWRRGTARGALAGMT
                     IGILAWAYTLLLPSLADAGIVGHSLLSLGPWGMALLRPQALIGLDLPPLAHGVIWSLS
                     LNALAYVVFSLGRAPASIERLQADLFVPSDLTPIPPSFRLWRSSVTVEELTTTVSRYL
                     GEERTRTSFDSYALTRGISIDPKAEADFQLLRYAEHLLASAIGAASSRLVLSLLLRKR
                     TVSTKAALKLLDDANAAIHYNREILQTALDHVRQGIAVFDKELHLVCWNRQFGEILAL
                     PPQLTRVGTGLDHILHFHAEQSTSDKNTADSLVRERLARYVASSEPFLQRFDERDLVM
                     EVRANPMPDGGIVTTFTDITPSVKAAEELERANESLERRVQERTEELTRLNTELGRAK
                     GEAEDANISKTRFLAAASHDILQPLNAARLYVTSLVERQRSGNDAQLVTNIDASLDAV
                     EEIFGALLDISRLDTGAMKPEIVGFRIDELLRQLEVEFTPLAQEKGLTLKFVPCSLAV
                     QSDRRLLRRLLQNLVSNAIKYTPQGRVLVGCRLRGGRLRIDVYDTGLGIPASKKRAIF
                     QEFYRLDQGAKAARGLGLGLSIVERIARVLDYRITVISAVGRGSQFSVEVPLSAVTPA
                     SQHQRVGRREVDRVQLSGIIVLCIDNDLTILDGMETLLAGWGCRVFKAPDLKAAIAII
                     AEAKMTPDGLLVDYHLDEGNGIAAIKELRRRFGADLTAILITADRSPHVREDARANAI
                     QLLHKPIKPAALRALLTQWRTQRVAAAE"
     gene            8669..9775
                     /locus_tag="VM910_03650"
     CDS             8669..9775
                     /locus_tag="VM910_03650"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HVI61677.1"
                     /translation="MPPPVSPLRELMQVMGTLLIAAVGGIGFYLVGFPGGLVSGSMLT
                     VAVAALGGCAVKIPLVLARLCFVLVGILLGAVVTPDTLKGIASWPLSVALLVVAAICM
                     MTATAWYLRLAHGWDPLSALLGASPGSMAQVMALAAEFEADVRGIAIVHVMRVLLIVL
                     GLPAGLALFGSTVEPVVSTRGATEPSLVELATLLGVSSFSALAMLRIRFPGGLMFGAM
                     AGSGLLHGTDLIQVSLPWWAGGAAVLTLGAVAGARFANTSPKMLLAYLAAAFGSFAVA
                     VAVAASFALVVVALLPFRIADVIVAFAPGAQDTMMVLALAMHLDPVYVGAHHLARFLA
                     VSFSVAVVARRLVRREPTRPREQWRRPGQGTFDD"
BASE COUNT         1919 a         2940 c         3225 g         1931 t
ORIGIN      
        1 ggcaataccg gcaccaatat attttgcggc cgtcgggtcc atagcaagct ccttttcgag
       61 ggttgtttga atgattggca ctcaatggcc tgggtgaagc gcatcattga ggtagatgca
      121 agtcaggatg gcgaagacgt acgcctgcaa caaagccacc agcagttcca gggcggtgag
      181 cgctgccgtc aaagctagcg gcaacgcggc gccgagccag cctaagactc cccaagcacc
      241 gagcatcaca acgaacccgc cgaacacctt cagcgtgatg tgaccggcga gcatgttggc
      301 aaataggcgt acgctatggg acaccggtcg cgaaaagaac gacagcacct cgatcgccgt
      361 taccagcggc aggatataga tcggtacccc ttttggcacg aagagcttga ggaaccgcaa
      421 accgtgtagc cagaagccgt agatgacgac cgtaaagaag acgagcaggg cgaacgcaac
      481 cgtaatgacg atgtggctgg tgaccgagaa ggtatacggg atgatgccta tcaggttgag
      541 caccaagatg aacatgaaga tcgagaatac cagaggaaaa aatttcattc cctccgtgcc
      601 cgtagtgctt cgcagcgtgt cggcgacaaa ttcgtacgac atttcggcgg ccgcctgtag
      661 ccgccccggc accagcgcgc gcgatctggt ggcaccaagc atcagcagga ggatcagcgt
      721 gagtccgatc agcatgaaca gagccgaatt cgtgaaggcg atctcgaccc cgccgatgcg
      781 gacgattgga aagaagtttt tgatctcaaa ctgatggatg ggatccatga tggatcaagt
      841 ccttcatcgc cgcgtagcca cttcggcgct tcgcgcgggg gcggtcgagt tcattgcttc
      901 gaaccaggcg ggtccaatgt gttgcttgct acgactcccg ccgcgcgcat cacgttgaga
      961 accccggctg cgaagccgag gagcagaaac acgatcatcc cccaaggcga aattccgagc
     1021 cagcgatcaa gcagccagcc gatggccgca ccgaccacga ccccggccac gaactcggtc
     1081 gagagccgaa aaccgcgggc gatcgctgac ggatcggcgg tcggacgttg gcccgtggca
     1141 ttttcggaag gacggtcagc atggacgcgg ccgagccgtt cgccgagacg cttaaacctc
     1201 gcggagagcg cagcctcgtc agccggcggt tgttcgcgat cacctttttc gcgtgcgttg
     1261 tcggccatgc tttcgacacc cgcgacggtt caggcaagat cacgatagtt ccgcccccta
     1321 aagccgcgcg gaccatactg actgggtcta tacgagtcaa gaattgcacc tccgcagtaa
     1381 atggttgaca atgaagtgaa atcacgaggc cggcgagcga tagacccagc gttttttcgg
     1441 cgatcccggc ggcgcgggtc ttgaatgcaa ggcggcatcc tttgcatctc cttcgccggc
     1501 ggcggtgacg tttcccgatt tggctcgcgc tcagtcttca cggcgggcgt cattgcatcg
     1561 ccctagagcg attgtcctat gggcgatatg cgaggaggga tcgcatgatc cgcacggctc
     1621 tattcgtggt cgctggactt gcgcttgcag tgccggcggc ttgggcgcaa agcacggcgc
     1681 cggagagcga cgatagccgc tacaccttca atcgcatcga tgatggctat ctgcggcttg
     1741 acggccgcac cggccaggta tcgatctgcg cgcggcaccc ggtgggatgg acctgccagg
     1801 cggtgccaga cgagcgcgcg gcgctcgagg ccgagatcgc acgccttcag ggcgaaaaca
     1861 tagcgatcaa gaaagagctg cttgcgcgca acctgccgct gcctggcacc gtcaagccgg
     1921 agccgccggc ggccaagccg gaggagcctc gcgtgcaatt gcccaacgat gccgatctcg
     1981 acaaggtcat gaatttcatc gagaagatct ggcgccgcct ggtggagatg atcgcgacgc
     2041 tgcggaagga catgctgaaa aagacctgac gatcgccgcg atggcaaggc catgagtgat
     2101 ccgttgtctc cgattatcag cttcgcgccc gagaccattg cgactgcgac actcgtggtc
     2161 gagaccagcg gacaagcctt caccgagatt acgcgcgagg cgatgcgatt catcaagcag
     2221 accggcgcga aggacggcgc gctgttcatc ttcatgcggc atacttcggg ctcgctcgtc
     2281 atccaggaaa acgccgatcc cgatgtacgc accgaccttg tgagcgcgct cgatcgcctc
     2341 gcgcccgccg atgcccagtg ggttcacgat gtcgaggggc ctgacgacat gccggcgcat
     2401 gtaaagacca tgctcaccgg agtgtcgctc cacgttcccg tgatcgcggg ggcgctcgcg
     2461 ctcggggtct ggcagggtat ttacgtcgcc gagcatcgcg cccggccgca tcggcgcgaa
     2521 atcgtgctgc aattcatcgg cagcaggcag taatcccggt tgtgtgttct cacgaacgcc
     2581 gatcgaagcc aaccagcggc acgccgtgag acaacgcgaa agtcgtcgtc atctcgccga
     2641 acaggcggtt gaactgtgcg tgctggtccc tgagaattgc cttgttcgcc ccgatggcgg
     2701 tgaaaacgct gaatgagctg atgcaccgtc atgccctgga gtttgcttcg cttcatggct
     2761 tgagaaaccg caaatctggg tcactaggtc ggtttgaaga tgcctcgatc gagagcgtcg
     2821 atcgtcattc cggcgtcgcc agcctgcgga tattcacgtg attgggccat gatttccagc
     2881 atgcgtcgcg cgcgctcggg tgccaccgct agggtcgcct tcacagcttt tagccggacc
     2941 tgtgcatggg gatggccgta gaggcaaagc agggcccgtc gttgatcacc gtcgcgtgcc
     3001 tttagttcct gttatctatc gcattgtcct gatccagggc gatggcggtg aaccgctcga
     3061 ctagctggtc caccgtcatg tcttggagtt tgctccgctt catggagagg ctcgctcgtt
     3121 tggatcatta tttgggcttg aagatacctt gctcgagatt ccatagcgac atccccgcgt
     3181 cgcctgcctg tggatattca cgcgaatcgg cgagggcctg aagtgcctgc cgggcgatct
     3241 caggcgatac agccaaagtt gcctttatgg cattcaatcg gacctgtgca ttgcgatgtt
     3301 tgtacaggga aagtagctcg cggcgctgat cgccatcgcg tcttttcagt tcgtcttcga
     3361 tggccaccat ttcgtcgaac aggcgattga actttccgta ctgacgcctg agaagtgcct
     3421 ggtcttgttc gatcccgatg ccggtgaaac gctccacgag ctggctcacc gtgatatctt
     3481 ggagtttggc ccgcttcatg gttttagaac tccgtgctgg atcagagctc taagaccaac
     3541 tctcgtccgt tcatcccagt ctttgccgcg taggtaatct ctgggcggga gtccgtcgta
     3601 gtctttattc ctcgtcatat accacccggt gatctcccag tgtttgaacc ttgatatcag
     3661 caccagattt tcgggagcat tgatcatgtc tctcggaaag cgatcctgct cggccgaggt
     3721 tttctcgacg atgtgatgaa tatcatatcc cggttttggc gttgagacag cctgctgcag
     3781 ttcctgcagc gtctttggcg gatcgagata ggcttggaca gacggctcgt attctaaaaa
     3841 ccagttgggt gcttcgacga cgtgcctttg ctcttgagcg acccatcgct gagcgagcca
     3901 tcgcgccgtc gctcttgcaa tgtcgtagtg ctccgacgcg gtcggcggcc tttcgcgtgg
     3961 aattatgggc gggggcggag ccgccagctc gcgcagcatc gcgcacggcc gcaccggcgc
     4021 gaaatcgtgc tgcagttcat cggcagccgg tagtaatccc ggcttgtctg tatttgcaag
     4081 aacccgatcg aaaccaaccg atggcaggtc gtgaacacga tccgggagtc gtgctatgct
     4141 atcgggtagt ggcgcccaag aacaaaggtt gcgcgggaga tgaagcgggc ggacgaattg
     4201 gtacgcttga tcatagcgga cgatcatccg ctgtttcgcg gggctttgcg cgaggctgtc
     4261 tcgggtctgt tcgaagaggt ggagattgcc gaggcaggat ccttcgacga tgtggcgaag
     4321 ctgctcgagc gcggtgcaga agtcgatctc atcctgctcg acttgacgat gcctggcgtg
     4381 cgcggatttt cgggcctcat gtatctgcgc gcccagtatc caagtgtgcc gatcgtcgtg
     4441 gtctcggcga acgacgatcc tggggtcatc cgccggtgca tggatttcgg agccttgggc
     4501 ttcatcccca agacgctcgg tatcgaggcc atgcgagggg cgatcagccg ggtattcgaa
     4561 ggcggcgttt ggacgccgcc cgacatcgac ctcggggccg gctccgatgc cgagacggcc
     4621 gacttgttga cacggctcac gtcattgacg ccccagcagg tgcgcgtgct gatgatgctg
     4681 tcggagggct tgctcaataa acagattgcc tacgaactca gcgtgtccga agcgaccgtc
     4741 aaggcgcacg tctcggcgat cttgcaaaag ctcggggtcg agagccgcac gcaagcggtg
     4801 atcgccgctt ccaagatcga gctcggccag tggccgcaga cggtaagcgc gggccattga
     4861 tttgttgaag tcgtggtaat ttcaccgatc gtgtgccgtc tcattccgcg gccgcgaccc
     4921 gctgcgtgcg ccattgcgtc aatagtgcgc gcaatgctgc cggcttgatg ggcttgtgaa
     4981 gcaactggat agcattcgcg cgggcgtcct cacgcacgtg cgggctgcga tcggcggtaa
     5041 tcaggatggc cgtgaggtcg gcgccgaagc gccggcgcaa ctccttgatc gctgcaatgc
     5101 catttccttc gtcgaggtgg taatccacca gcaagccgtc aggcgtcatc ttggcttctg
     5161 cgatgatcgc gattgctgcc ttgaggtccg gcgccttgaa gacccggcag ccccatccag
     5221 cgagcagcgt ttccattccg tcgagaatcg tgaggtcgtt atcgatgcac agaacaatga
     5281 tgccggagag ctgcacgcga tcgacctccc gtcgtcccac gcgctgatgc tggctcgcgg
     5341 gagtgacggc ggagagtggc acttcgaccg agaattgcga cccacggccg accgctgaga
     5401 tcacggtgat cctgtagtcg aggacgcgcg cgatgcgctc cacgatcgaa aggccgagcc
     5461 ccaagccgcg tgcagccttg gcaccctgat cgagccggta gaattcctgg aaaatcgccc
     5521 gctttttcga agccggaatg ccaagtccgg tatcgtagac gtcgatgcgc aatcgtccgc
     5581 cgcggaggcg gcagcccaca agcacgcggc cctgaggggt gtatttgatc gcgttggaga
     5641 cgagattctg tagcaaccgg cgcaataaac gccggtcgga ttggaccgcg agcgagcacg
     5701 gcacgaattt gagggttagt cccttctcct gcgcgagcgg cgtgaattca acctcgagct
     5761 ggcgcaaaag ctcgtcgatg cgaaagccga cgatctccgg cttcattgct ccggtatcaa
     5821 gccgagaaat gtcgagcagt gcgccaaata tttcttcgac ggcgtcgagc gaggcgtcga
     5881 tatttgtgac cagctgggca tcgttgccgc tgcgctgccg ctcgaccagg ctggtgacgt
     5941 aaaggcgagc ggcattgagc ggttgcagaa tgtcgtggct tgccgcagcg aggaaacggg
     6001 tcttggagat gttggcgtct tctgcctcgc ccttggcacg gccgagttcg gtattgaggc
     6061 gggtcaattc ctcggtgcgc tcctgcaccc gccgctccag cgattcgttg gcgcgctcaa
     6121 gctcctccgc cgctttcacg ctcggggtaa tgtcggtgaa ggtcgtgacg atgccgccgt
     6181 ccggcatggg gttcgcacgc acctccatca ccagatcgcg ctcatcaaaa cgttgcagaa
     6241 acggctcgct gcttgcgacg taacgggcaa gccgttcgcg cacgagcgaa tcggcggtgt
     6301 ttttgtcaga agtgctctgt tctgcgtgga agtgcaggat gtggtcgagg cctgtgccaa
     6361 cccgagtgag ctgcggcggc aatgcgagga tctcgccgaa ctggcgattc caacagacga
     6421 gatgcagctc cttgtcgaat accgcgatgc cctggcgaac atggtcgagc gcggtctgta
     6481 aaatctcacg gttgtaatgg atggcggcgt tggcgtcgtc caggagcttg agcgcggcct
     6541 tggtcgagac cgtacgtttg cgcagcagga gcgagagcac gaggcgggac gatgccgcgc
     6601 cgatcgcgga cgcgagcaga tgctcggcat agcgcaggag ttggaagtcc gcctcggcct
     6661 ttggatcaat gctgatgccg cgtgtcaagg cgtagctgtc gaaggaggtg cgtgtgcgct
     6721 cttcgccgag gtagcgggag acggtcgtgg tcaattcctc gaccgttacc gaggagcgcc
     6781 acaaccgaaa actcggcggg atcggagtca agtcggaggg aacgaagagg tcggcctgca
     6841 gccgctcgat cgaggccggc gcccgtccaa gcgagaacac cacataggca agagcgttga
     6901 gcgaaaggct ccagatcacg ccgtgggcga gcggtgggag atcgagtccg atgagcgcct
     6961 gcgggcgcag cagcgccatt ccccacgggc ccagggagag caggctgtga ccgacgatac
     7021 cggcgtccgc caagctcggc agcaacagcg tgtaggccca ggccaggatt ccgatcgtca
     7081 tccccgccaa cgcgccgcgt gcggtacccc gtcgccagat cagtccgccg aaaaaggcag
     7141 gcgcgagctg cgcaaccgcc gcaaaggaca acaggccgat cgaagcaagc tgcgcatcgc
     7201 ccgccaagcg atagtagacg taggcgagaa acagaatgac gaagatcgcc attcgccgca
     7261 cggtcagcag caagccaccg atattgtctc gcccggtgat cagcgcctcg cgccgctgca
     7321 gcacgagcgg catgacgatg tcgttcgaca ccatgatggc gagcgcgacc gattccacga
     7381 tcaccatcgc ggtcgccgcc gagaggcctc cgacgaaggc tgcgatcgta aacaggtcgg
     7441 aatgcgcggc gagcggcaag gccaggacga acatgtcgct gtcgacctgc cccggcgtga
     7501 atgtcagcag gccggccaat gcgatcggta cgacgaacag attgatcagc acgagataga
     7561 gcgggaacag ccaagaagcg cgcctgatct cgttctcgct gttgttctcg accacggtga
     7621 cgtggaactg gcgcggcagc aatatgatgg cgaacagcga cagcagcgtc attgcgagca
     7681 gtgtgcccgg tgccggctcg cgggtgagca cggccgcggt gtccggccgc tccatggctt
     7741 gggcaaagag tatgcgcggc ccgtcgaaca tccagaacgt gacgaatatg ccgacaccga
     7801 cgaacgcaac gagcttgacg attgactcgg ttgcgatcgc cagcatcaat ccgtcttgat
     7861 gctcggtagc atcgatatgg cgcgtgccaa acaagacggc aaagatcgcc attgccaccg
     7921 ccacgtagag cgcgatgtcg cccaacaccg gctgcatcgc gccgctcgcg ggtccgatat
     7981 gcacgaggat tgtggtcagt gaagacgata ccgctttgag ctgaagcgcg atgtagggaa
     8041 tggtgccgat gatcgcgatc agcgccacag tagcggcgac ggtctgggct ttgccgtaac
     8101 gggcggcaat gaagtcggcg attgacgtga tgttctgtgc cttggccagc cgaacgatgc
     8161 gcatgatcag cggcgagcac agcccgacca gcaggatcgg cccgatataa atggttaaaa
     8221 agtcaaagcc ggtgcgcgag gcgagcccca ccgagccaaa gaacgtccag gaggtgcagt
     8281 agatcgcgag cgaaagcgga tagatgaagt ggcgccaccc gccttcgcgg ccgaaccgcc
     8341 gggtccggtc cccgtagctc gcaaccacga acaagaggcc gatatagccg agtgcgacgg
     8401 cgataacgat ccagccttgc agcatgatgt ttccacgggt ccttggggac gcgaagcccg
     8461 ccgatgcatt ctaccgcacg aatggggcag cgagaatatc gctggcagct tggcggcact
     8521 ccccgccggg gtgtcgaggg gagttttgat gtgacgtggc agatgggccg ccatttgacg
     8581 cggcgcaccg caggcggcat tgatcggtga ttaaaagcgc accgcgggac gcgccttgcc
     8641 cggcgacccg agcgtccggg accaatcgat gccgcctcct gtttccccac tgcgcgagct
     8701 catgcaggtt atggggacgt tgctcattgc cgcggtgggt ggaataggat tctatctcgt
     8761 cggctttcca ggcggactgg tttcgggctc gatgctgacg gtcgcggtcg ccgcgctcgg
     8821 gggctgtgcc gtgaagatac cgctggtcct cgcgcgcttg tgctttgtgc tggttggtat
     8881 tctgctgggg gcggtggtca caccggacac gctcaagggg attgcgagct ggccgctgag
     8941 cgtcgcactg ctcgtcgtcg ccgcgatctg catgatgacc gctaccgcct ggtacctgcg
     9001 cttggcgcac ggttgggacc cgttgtcggc tttactcggt gcgagcccgg gctcgatggc
     9061 gcaagtgatg gcgctggcgg ccgagttcga ggccgatgtg cgcgggatcg cgatcgtgca
     9121 cgtgatgcgt gtgctcttga tcgtgctcgg cctgcccgcg gggcttgcgc tgttcggctc
     9181 gaccgtagag cccgttgtgt cgacgcgggg cgcgacagaa ccttcgctcg tcgaacttgc
     9241 gaccctccta ggggtatcga gcttttcggc actcgccatg ctgcgcataa gatttccggg
     9301 cggcctcatg ttcggcgcca tggccggctc aggacttctt cacgggacgg atttgatcca
     9361 ggtgtcactg ccctggtggg caggcggggc cgccgtgctc acgctgggcg cggtcgcagg
     9421 cgcgagattc gcgaatacga gccccaagat gctgctggcc tatctggcgg cggcctttgg
     9481 ctcgtttgcg gtcgccgtcg ccgttgccgc atcgtttgca ctggttgttg tggccttgct
     9541 cccgtttcgg atcgccgacg taatcgtcgc cttcgcgccg ggggcgcagg acacgatgat
     9601 ggtgctcgct ctcgcgatgc atctcgaccc ggtctatgtc ggagctcacc acctcgcacg
     9661 gttcttggcc gtcagctttt cggtcgccgt ggtggcgcgg cgcctcgtcc gccgcgagcc
     9721 cacccggccg cgcgagcagt ggaggcggcc cgggcagggg acgtttgatg actgacgtag
     9781 gccacgctga cgggagccag ccgcctgtgg tgtgtcggct tccgaacgtc caggcgagcc
     9841 gttatcattg cgctgatcgt ctggactgcc cgcgcgccgc gtctgacgga gaaacaacat
     9901 gcatgacaca accgacggca gccactggca cgagccggaa gggttgaaga acgcaggata
     9961 cggcgcttgg aagcggccca atacgccgta tgacgactac atggaggcgc agggg
//
LOCUS       DATLJT010000164         6553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3683840,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000164 DATLJT010000000
VERSION     DATLJT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3683840"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1162)
                     /locus_tag="VM910_03655"
     CDS             complement(11..1162)
                     /locus_tag="VM910_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI61678.1"
                     /translation="MFRRDTPAETAFRMEVRTWLEANLPVALRGRITRPPPGELMPWY
                     HTLSRKGWIAPHWPKQYGGMGATLNEQLIMTEELARIGAPHLPVQGLNHIGPILMEFG
                     TAAQKAKHLPPIIAGTVIWAQGYSEPGAGSDLASLSTRATLGGDHFVVRGHKIWTTWA
                     HHSDWMFALVRTDPQAQPRHAGISFLLIDLHSPGIRIRPIKTIVGDEEFSEVFLDDVV
                     VPVENLVGKLHDGWRIANALLGHERLATSNPQFSLMALERIKTMARTSGIIADPAFQD
                     RLAAASINVMALSALFSHAVELTNHEKSPGPESSVIKIVACELLQALNDLLVEAAGGH
                     ASADEPIASNFGEVDVAAAFLQSRRATIYGGSSEIQRNVLARRVLNLPS"
     gene            complement(1222..2196)
                     /locus_tag="VM910_03660"
     CDS             complement(1222..2196)
                     /locus_tag="VM910_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61679.1"
                     /translation="MRALLAASLCAGWALAGTGLAHAQAYPTRPIRIVVPFPAGGPTD
                     GMARIVSDRLGAVLGQTIVVENHGGGAGGSIGAKAVATADPDGYTILMTPGGALTTGP
                     AVHRNIGYDPTKAFTPVGLLMVTPNIISVHPSLSVKSLAEVVAYAKANPGKISWGSQG
                     FGTAPHLLAEMFKLETGVNIVHVPYRGTAPLLAAAVAGEVQIIADPATSSLPHIQSGN
                     LRALAIAGAERFSKLPDVPTTPEAGYPKLQAPFWLGVVAPAGTPAAIIDKLNAAFRDS
                     LAQPQTRARLATLGAEIKIGTPAEFGKLLANELAQWTAVVKAANIKVE"
     gene            complement(2354..3850)
                     /locus_tag="VM910_03665"
     CDS             complement(2354..3850)
                     /locus_tag="VM910_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVI61680.1"
                     /translation="MNDQTISTTSQSLPRASADGQRAARTTLLLKGRLLSTLLRLSAP
                     NVLNLLAIAGMITFDGLFLGRLGPDALAGVSLAFPFVMLIQHTAASGMGGGVSSAIAR
                     ALGASKRDVADALVFHAFVLALALAAAFSTVLLLASPFIFRWMGGHGEILWTALAYSN
                     VAFSGAVSICMLNLLGSAVRGTGNMGLPAGVIVGSVLAHVVLSPILIFGWGPLPALGP
                     AGAGWGLAGTFGAGSLVLLFYLRSPRSLVTLRFRGVALRQALFADILKVGVPGLINVI
                     ITNLSVMLLTGIAGRLGREVAIGYAMGARLEYILIPLAFGFGTAIVAMVGTNWGAKQY
                     RRAYQIAWTGAATVGASCAAIGLIVALCPTLWMDLFSKDPQIIRIGSWYLQIVGPIYG
                     FYGLGMALYFATQGVGRVALTVTANAVRLVASAAFALIAVYVFDLGAIGFFAAVAIGF
                     VMYAALTVCAVVRLKSGPVAPKLEPLARRESKEELSGASTPPIYSASS"
     gene            complement(4102..5058)
                     /gene="gcvA"
                     /locus_tag="VM910_03670"
     CDS             complement(4102..5058)
                     /gene="gcvA"
                     /locus_tag="VM910_03670"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVI61681.1"
                     /translation="MRAAPPLNALRAFEAAARHLSLTKAAHELNVTPGAISHQIRGLE
                     TLLGVELFERRARSIALTPAGKLLHPGVQTGLLYIREAVANIQGARQERILVVSTPPG
                     LTSKWLAPRLYRFTSSYPDIDVRISSSIDNANFTTDGVDVAVRNLPIDPRSDPALVIE
                     KLVELSFVPVCSPRVIERYGPLRTAKALARAPLVHDDTLASRAAVPTWADWFRAAGVE
                     GVNVSRGLRFNSSDHALDAAGEGAGVLLAHDLLAYDDLRSGRLLIPVALALPSGRAYH
                     LVRASGRRNRPQVLAFGGWIKKELAALDWTSIRGAARLRRTS"
     gene            5184..5411
                     /locus_tag="VM910_03675"
     CDS             5184..5411
                     /locus_tag="VM910_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61682.1"
                     /translation="MNAIAPRHDFARGHAIATPRDAPLRSGFLRCIVDTIVRVRRHRE
                     ERELARLFNRYGRHLTDEVERRIFEEMTRRR"
     gene            5455..6246
                     /locus_tag="VM910_03680"
     CDS             5455..6246
                     /locus_tag="VM910_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVI61683.1"
                     /translation="MSEIVLVEKEGPLALITLNRPEKLNALDYALIDRLMEVLDTVED
                     DDGIRALVLTGAGNRAFSSGADIACFAASVRRGQAVALREFVRRGQRLTARIETFPKP
                     IIAAVNGLAHGGGCEITEAAPLAIASDRAEFCKAEINLGFAPPFGGTQRLPRLIGRKR
                     ALGMILTAEPITASEACALGLVNDVVPHERLLEAAFALAHKIADKSPLAVAACLGSVT
                     RGINVPIDEGLAIEANYFARMVPTRDIEEGLSAWLERRKPQFMGC"
     gene            complement(6329..>6553)
                     /locus_tag="VM910_03685"
     CDS             complement(6329..>6553)
                     /locus_tag="VM910_03685"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HVI61684.1"
                     /translation="SYAVCMAYLRGLFDGMQQAKIVEDTKSTFCPPQTPDLEQIRLII
                     EQWVRDNPKNAGVSVGWAGPLALSLAFPCR"
BASE COUNT         1282 a         2015 c         2087 g         1169 t
ORIGIN      
        1 gtgccgtccg tcaactcgga aggttcaaca cgcggcgggc gaggacgttg cgctggattt
       61 ccgatgagcc gccgtagatt gtcgcgcggc gcgactgaag gaacgctgct gccacatcca
      121 cctcgccgaa attgctagcg atcggctcgt ccgccgatgc gtgcccgcct gccgcctcaa
      181 ccaatagatc gttgagcgcc tgaagaagct cacacgcaac aatcttgatc acggacgatt
      241 ccggcccggg actcttctcg tgattggtga gctccacggc atggctgaac agcgctgaca
      301 gcgccatgac gttgatactc gccgccgcga gacgatcttg gaaagccgga tcagcgatga
      361 taccggacgt gcgcgccatc gtcttgatgc gctcgagcgc catcaacgag aattgcgggt
      421 tcgaggtcgc caagcgttca tgtcccagca gcgcatttgc gatgcgccag ccgtcgtgca
      481 gcttgccgac caggttttcg acggggacga cgacatcgtc gagaaatacc tcgctgaact
      541 cctcatcacc cacgatcgtc ttgatcggtc tgatcctgat gcctgggctg tgcagatcaa
      601 tcagcaggaa actgatgccg gcgtgccgcg gttgggcctg cggatcggtg cgcaccagcg
      661 cgaacatcca atccgagtga tgtgcccagg tcgtccaaat cttgtgcccg cgcaccacga
      721 aatgatcgcc cccaagcgtt gcccgggttg aaaggctggc gaggtcggag cccgcgccgg
      781 gctcggaata gccctgcgcc cagatcacgg tgcccgcaat gatgggcggc agatgcttgg
      841 ccttctgtgc ggcggtcccg aattccatga gaatggggcc gatatgattg agaccttgaa
      901 ccggcagatg cggcgcgccg atgcgcgcca gctcctccgt catgatcagc tgctcgttga
      961 gggtggcgcc catcccgcca tattgcttgg gccagtgcgg cgcgatccag cctttgcggg
     1021 agagcgtgtg ataccagggc atcagttccc cgggcggcgg acgcgtgatg cggccccgaa
     1081 gcgcgaccgg aaggttggct tccagccatg tccgtacttc catacggaat gcggtttcgg
     1141 caggcgtgtc acgacggaac atggcgatga aattcttcgc tgctgatgcg ggcgaaagga
     1201 tagggcgcgg aacgccgcac ttcattccac cttgatgttc gccgccttta cgacggcggt
     1261 ccactgcgcg agctcgttcg cgagcagctt gccgaactcg gctggcgtgc cgatcttgat
     1321 ctcggcgccg agggtcgcga gtcgcgcgcg cgtctgcggc tgggcgaggc tgtcgcggaa
     1381 cgcggcattg agcttgtcga tgatggcggc aggcgttccg gccggagcca ccacacccag
     1441 ccagaatggc gcctgcagct tgggatagcc cgcctccggc gtcgtcggca catcgggcaa
     1501 tttcgaaaag cgttcggcac cggcgatcgc cagggcacgc aggttcccgg attggatgtg
     1561 gggcagactg ctggtggccg ggtcggcgat gatctgaacc tccccggcca ccgcggcggc
     1621 gagtaatggt gctgtgccgc ggtagggcac gtgcacgatg ttgacgccgg tttcgagctt
     1681 aaacatttcg gcgagcagat gtggcgcggt gccgaagccc tgcgagcccc agctgatctt
     1741 ccccggattg gccttggcgt aggccacgac ctcggccagc gattttaccg acaggctcgg
     1801 gtgcacggaa atgatgttcg gcgtcaccat gagcaggccg accggcgtga acgctttggt
     1861 cgggtcgtag ccgatattgc ggtgcacggc cgggccggtc gtgagcgctc cgccgggagt
     1921 catcagaatg gtgtagccgt ccggatcggc ggtcgcgacc gccttggcgc cgatgctgcc
     1981 gccggcaccg ccgccgtggt tctcgaccac gatggtctgg ccgagtacgg cgccgagccg
     2041 gtccgagacg atgcgggcca tgccgtcggt cgggccgccg gccgggaagg gcacgacgat
     2101 cctgatcggc ctggtgggat aggcctgggc gtgcgcgagg ccggtgcccg cgagcgccca
     2161 tccggcgcac agcgatgccg caagcagcgc tctcatcatc ttaagtcgcc tcccgcgtgg
     2221 tcgggcaatg cgggcggtat tctatcgcgg aattcccgag ataactctta gccgggcgtg
     2281 aatattttgg gccttgcagt atccgctcgg cgcatcggcc tgcggtcaac ttctttcggg
     2341 ggccacgcca gggctagctg ctagcagaat agataggtgg agtggacgca cctgataatt
     2401 cttctttgga ttcgcgccga gcaagtggtt ctagtttggg cgccacgggg cccgacttca
     2461 accgcacgac ggcgcacaca gtcagggctg catacatgac aaatccaatc gcaacggcag
     2521 cgaaaaatcc gatcgccccg agatcgaaca cgtatacggc aatcaatgcg aatgcggcac
     2581 ttgccacgag ccggaccgcg ttcgcagtca ccgtcaaggc aaccctgcca actccctggg
     2641 tcgcaaagta gagcgccatt cccagaccgt agaatccata aattggtccg acgatctgaa
     2701 ggtaccaact gccaatgcgg atgatttgag gatccttgct gaagagatcc atccacaagg
     2761 tgggacacag cgcgacgatc aatccaatcg ctgcgcatga ggccccaaca gtcgcagctc
     2821 cggtccatgc gatctgatat gcgcggcgat actgcttggc accccagttc gtaccgacca
     2881 tggcaacgat ggctgtgcca aatccgaaag ccaagggaat caggatgtat tcgaggcgag
     2941 cccccatcgc gtagccgatc gcgacctcgc gccccaggcg cccggcgatc cccgtcagga
     3001 gcatgacaga aaggttagtt atgatcacgt tgatcaagcc tgggacaccg accttcaaga
     3061 tgtctgcgaa caaggcctgt cgcagggcca cgccgcgaaa cctcagcgtt acgagcgaac
     3121 ggggggaacg cagatagaac aggagcacga gactgcccgc accgaaggtg cccgcgagtc
     3181 cccaacccgc gcctgcaggt ccgagtgctg gtagcgggcc ccacccgaag atcaggatcg
     3241 gtgagaggac aacgtgtgcg agcacgctgc cgacaatcac gccagcgggt aatcccatgt
     3301 tccctgtgcc ccgcacggcg cttccgagca ggttgagcat acagatggat actgctccgc
     3361 tgaacgcgac gtttgagtag gccagcgccg tccacagaat ttcgccatgt ccgcccatcc
     3421 atcgaaagat gaatggtgaa gcaagcagaa gcacagtgga aaacgctgct gcgagcgcga
     3481 gcgcaagcac gaacgcgtgg aagacaagtg cgtcggcgac atcgcgcttg gaagcaccga
     3541 gggcacgcgc aatcgcggac gaaacaccgc cgcccatgcc actcgctgcg gtatgctgaa
     3601 tgagcatcac gaaaggaaaa gcgagcgaca cccccgcaag cgcgtcgggg cccagccggc
     3661 ccaagaacaa accatcaaac gtgatcatcc ccgcaatcgc gagcagattg aggacgttgg
     3721 gggcggacag tcggagcaat gtcgataaaa ggcggccctt aagcagcaac gtagttcgcg
     3781 ccgcgcgctg accgtcggcg gacgcccgag gcaatgactg cgaggtcgtg gaaatagttt
     3841 ggtcgttcat atgctcctcc cgatacgggg ttagagcagt catcgacgcg cttgtgtctc
     3901 gaacaatcga acagctgctg aacagcccga tcgcgcgctt cgaagctagg ttggatatgt
     3961 ttgcaatcgc cccgacagcg caacgaatca attttcaggc cggtcttaga aattctcatg
     4021 cagcggggac atgaacgacg ttgccgcgtt cgccgccgct tatctgtgac gggacatgat
     4081 tcttgctgcg gcaccccctg gtcaggacgt ccgtcttagc cttgctgctc cgcgaatcga
     4141 agtccaatcg agcgcggcga gctccttttt gatccagcct ccgaaggcca gcacctgcgg
     4201 gcgattacgg cggccgctgg cgcggacgag gtgataagct cggcccgagg gcagcgcgag
     4261 tgcgaccggg atgagcagcc ggccggagcg caggtcgtcg tatgcgagca gatcatgtgc
     4321 gagcagcacg ccggcgccct cacccgcggc atcgagcgcg tgatcggagc tattgaagcg
     4381 cagcccgcgg ctcacattga cgccctcaac gcctgccgct ctaaaccagt cggcccatgt
     4441 cggcaccgcg gcacggctgg cgagcgtatc gtcgtgaacg aggggcgcgc gggcgagggc
     4501 ctttgcggtc cgtaagggcc cgtagcgttc gattactcgg ggactgcata ccggtacgaa
     4561 cgacagctcg acgagcttct caatcaccag cgctgggtcg gagcgcggat cgatcggcag
     4621 attgcgcact gcgacgtcga cgccatcggt cgtgaaatta gcgttatcga tcgaggagga
     4681 gatgcggacg tcaatgtcgg ggtagctgct ggtgaatcgg tagagccgcg gcgcgagcca
     4741 ttttgaggtg agacccgggg gcgtgctgac gacaaggata cgctcctgcc ttgcaccctg
     4801 gatgttggcc acggcctcac gaatgtagag gaggccggtc tgcacgccgg gatggagcag
     4861 cttgcccgca ggggtgagcg caatcgaccg ggcgcgccgt tcgaacaatt cgacgccgag
     4921 cagcgtttcg agcccacgga tctggtgact gatggcgccg ggcgtgacgt tgagctcgtg
     4981 cgctgccttg gtcaggctta agtgccgcgc cgccgcttcg aaggcccgca aggcattcag
     5041 tggcggcgcc gcacgcatga gaaaagctca cttatatccc gagaacttat cctttgtcat
     5101 agcggcaaac cgcggtcaac atccatggca cctgaggttt tcgaatgaga acgcctcaaa
     5161 gattgagacc gggatacaca gccatgaacg ccatcgctcc tcgccatgat ttcgctcgcg
     5221 gccatgccat tgcgacgccg cgggatgccc ctcttcgcag cggattcctc cgttgtatcg
     5281 tcgataccat cgtgcgcgtt cgccggcatc gggaggagcg cgagctcgcg cgtctgttca
     5341 atcggtacgg ccggcacctg accgacgagg tcgagcgccg gattttcgag gagatgaccc
     5401 gcaggagatg acccgcaact gcaattttcg ggtctgaggg ccgagcggac cgcgatgtcg
     5461 gaaattgtcc tggtcgagaa ggagggaccc ctcgcgctga tcacgctgaa tcggcccgag
     5521 aagctcaacg cgctcgatta tgcactgatc gatcgactga tggaggtgct cgacaccgtc
     5581 gaggacgatg atggtatccg cgcgctcgtc ctgaccggcg ccggcaaccg tgctttttcg
     5641 tccggggccg atattgcgtg ttttgcggca agcgtgcgac gcggccaagc ggtcgccttg
     5701 cgagagttcg tacgccgcgg gcaaaggttg accgcaagga tcgagacatt cccaaagccg
     5761 atcatcgcgg cggtcaacgg gctcgcccat ggcggaggct gtgaaatcac cgaagccgcg
     5821 ccgctcgcca tcgccagcga ccgcgccgaa ttctgcaaag ccgagatcaa tctcggattt
     5881 gcgccgcctt tcggcggcac gcagcgactt ccacggctga tcggtcgaaa gcgcgcgcta
     5941 gggatgatcc tcaccgccga gcccatcacc gcctcggaag cctgcgctct gggtctcgtc
     6001 aacgacgtag tcccccacga gcgtctcctc gaggcagcat tcgccctcgc gcacaagatc
     6061 gccgacaaat ccccgctggc ggtcgcggcc tgcctcggaa gcgtgacgcg cggcatcaac
     6121 gttccgatcg acgagggtct cgcgatcgag gcgaactact tcgcccgaat ggtgccgacg
     6181 cgcgacattg aggaaggact ttcagcgtgg cttgaacggc gcaaaccgca attcatgggt
     6241 tgctaggttt cttggcccat ccggcggcgc tgcatcggct ttcttgatgg gcgtgccgtt
     6301 gctcgcccgc tccagcactt ttcaggttct atcggcaggg gaaagccagc gaaagagcaa
     6361 gcgggccggc ccaaccgaca gacacaccgg cgtttttggg attatctcgg acccattgct
     6421 cgataatcag ccgaatttgc tccaagtcgg gagtttgcgg tgggcaaaag gtactcttcg
     6481 tgtcctccac gatcttcgcc tgctgcatgc cgtcaaacag gcctcgaaga taggccatac
     6541 aaaccgcata cga
//
LOCUS       DATLJT010000165         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3913832,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000165 DATLJT010000000
VERSION     DATLJT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3913832"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VM910_03690"
     CDS             <1..246
                     /locus_tag="VM910_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61685.1"
                     /translation="QEGPVRGGGGTAVMSPALKLLIDGVRIVMQVLVDEFGDRMTRDT
                     REDIQQWYDRCYEMLGEGQREQVSDRTDSDSRPHEFG"
     gene            643..825
                     /locus_tag="VM910_03695"
     CDS             643..825
                     /locus_tag="VM910_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61686.1"
                     /translation="MMRPTTTPFGKHAEVVVVALAAEVVVVALAGRAGSQNALEQHHA
                     RSRSVVSLDHLVGAGL"
     gene            complement(793..1062)
                     /locus_tag="VM910_03700"
     CDS             complement(793..1062)
                     /locus_tag="VM910_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61687.1"
                     /translation="MAQQHRLPAIYGFRQYVMEGALISYGPDTIDIYRRSASYVDRIL
                     NGEKPADLPVQAPTNFALAINLRTAKALGLTVPMTLQARADEVIE"
     gene            complement(1782..1985)
                     /locus_tag="VM910_03705"
     CDS             complement(1782..1985)
                     /locus_tag="VM910_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61688.1"
                     /translation="MSESDLIALGRLPFERDIFGSYSGALGQPEACCGVVSNGCVSAL
                     NEAAIQSVGCAAATTKTSHRLDE"
     gene            3175..>3789
                     /locus_tag="VM910_03710"
     CDS             3175..>3789
                     /locus_tag="VM910_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61689.1"
                     /translation="MSKAYPGVTALEDVSFSLRRGEIRALCGENGAGKSTLVEILMGL
                     VAPDRGSIAVNGQTQVMRSPQQAQGLGLGLVAQELSLAPHLSILDNIWLGSAEVPLLH
                     RRAKFRLRAQQALATLGLADWDLNRPVGSLTIAQRQLIEIARLLARDARVLILDEPTA
                     TLSDADIDRILGILRGLRAQGRSIIYVTHRLGEVFELCDSVTVLR"
BASE COUNT          711 a         1142 c         1198 g          738 t
ORIGIN      
        1 caagagggac ctgttcgagg aggtggaggg acggccgtga tgtcgcccgc cctgaaactg
       61 ctgatcgacg gagtgaggat agtgatgcag gtcctcgtcg acgagttcgg cgacaggatg
      121 acgcgagaca ccagagagga catccagcag tggtatgatc gctgctatga gatgctcgga
      181 gagggtcagc gtgagcaagt tagcgatcgc actgacagcg acagtcgccc tcacgagttc
      241 ggctaacgct ttggtctgac atctggcggc ccacgccggc cggcgccgag cttcgcctcg
      301 acaacggcgt cacggtgctt gccaagtttg atggcgaatt tgccgcccac tcctccacct
      361 acgccggcac gggtacgctt cgctacagtg agtcttgtgt cagacatgga cacgaagccc
      421 gaacacaaca gatcagagca agcccgcctg cgcgcaaagg aaacacggac ccgcacgggt
      481 tttgaaatcg aagccgctac tctcgccgcc agctcttagc aaatgctgcc atcgcggcct
      541 cgcgtgtcgg ctcgtagccg tgcgtcgtcc aggggccctc gtgatggacg aaagcgagcc
      601 atccacagcc acggcgcccg gaccggcgcg actgcggcct tcatgatgcg gccaaccacg
      661 acgccgttcg gcaagcacgc cgaagtcgtc gtcgttgcac tcgccgccga agtcgtcgtc
      721 gttgcactcg ccggaagggc gggaagccaa aacgcgcttg aacaacatca cgctcgatcg
      781 cgctccgtcg tttcactcga tcacctcgtc ggcgcgggcc tgtagggtca tcggcacggt
      841 gaggccgagc gcttttgcgg ttctcagatt aatcgccagg gcgaagttgg tcggcgcttg
      901 caccggcaga tcggcgggct tctcgccgtt gagaatgcga tcaacatacg aagccgagcg
      961 ccggtaaatg tcgatcgtgt ccggtccata agatatcagc gcgccttcca tcacatactg
     1021 ccgaaagcca tagattgcag ggaggcggtg ctgctgcgcc aatgcaataa tcgccccacg
     1081 atggggtgtt gatgaatggt tccggtggga cgatcaggcc gccaccgggc tgccgtgcaa
     1141 aggcggtggc cgccgcctct atctctgctt cgtcgcgcac cggtgttgcc gaaacttcgg
     1201 cggcaagtga tgacgcggcc gctcctaatt cgcgaaggaa ggcgggataa tagggcgcgg
     1261 tctgcggatt gaatatgagt gccgtccgcc tcaggtccgg ggcgatctcc ttgagcatct
     1321 ccagccattc agggggaatt tgttgcgcgc tcgccgagaa tggccacgcg gctgccgcgc
     1381 cgccgagcag cgtggctaag aattttcgtc gtccgatgtg gcttgccatg tgaccctccg
     1441 gttggggtca ttcatgcaat ggagggacta taccacgctt ccatcgcgcg atctgcggac
     1501 taaggccgat aggcacgccg agtgaatgct tccaaatgac cgtttaggta agaaccgttt
     1561 accggtttgc ccccgaggac aaatagcaga cgtccttgag ccattggcgg cgctggcgct
     1621 ggcgaaccgc ggtggcggcg atggcagagg tgagcgagcg cggcgtcggt gagcgcgagc
     1681 atttaacttt gagatgagct tacgccgctg aaatgcaacg cgatcgcgtt gacggcactc
     1741 tatgcctgct tgtccgagtg gtttcggcaa gtcaggtcgc ttcactcgtc taaacgatgg
     1801 ctggttttgg tggtggcagc ggcgcagccg acggattgaa tagcggcttc gttcaatgcc
     1861 gacacgcagc cgttgctgac gacaccgcag cacgcttcag gctggccgag agcccccgag
     1921 taggacccaa aaatatctct ttcaaatgga aggcggccga gcgcgatcag atcgctctcg
     1981 ctcatgacca cctcatcgcg gcacgcaaga cacgcgcgcg ttcgtctttc gttgcggtgg
     2041 ggtcacatgc tgcggaaaag cgcttgttgc tcaatgtgcg ccggaagcgc cacgtggggt
     2101 cactttccgc taaaaagttt tgatgaatca acgaacggct taatctgttc tgaatcgagt
     2161 tcaggtgctg ccgacgcccc aaaatgatac cacaccccga tgcgccagca gaccgctgca
     2221 ctgcaggatt ttggtcccct caatgtccgc tcaggacaca tcgcaatggg tgacagcgac
     2281 ctggtcattg tccgctttga cccactttgc ggactcaagt cggacatgtt cccttattcg
     2341 accacctcat cggcaagtgc gagcagtgtg ttcggtacgt tgagccccat cgcctttgcg
     2401 gttttccgat tgagcgcgaa caggaagcgc gtcggctgct cgaccggcag gtcggcggtt
     2461 tagtgccttt gaagatgcga tcgaccagag cggccgaacg ctcgaaaatc tcgcgccggt
     2521 ccggtccata cgacatcaag ccgcattcac gagttagcga atcggtctcg taaatcgctg
     2581 gcagccgtcg ctcggcagcg tactcgaata cgcgcctgcg attgcgaagc gtgagcgagt
     2641 ctgagaccat taagatggcg tcgggccgct cgcgattcat cgtatcaaaa gccttgatga
     2701 aatcgtccgg ctcgctcact cccagtggct ggatgatgat cccaagctcc cgcgcagcca
     2761 ccgccgatgc gtcgtagcgc agcgacatgc cgcggtcgcc cttgttccac agcattgcga
     2821 cccggcgcag ttgcggcaac agctccttga gcagagacag acgcttggtg gaaagcgtcg
     2881 tgctacgtcc gataggccag tgacgttgcc gcccggccgc gcgatgcttg caacgaggcc
     2941 gctgccgaca ggatcggggg gatagatggc cacgcaacct caccgtgtgg cgttttcgcg
     3001 gattgcacca tcactgcggg cggatatgat tttcgggaag gacagggact gcagatgtgt
     3061 aggttgacgc gccgtgcacc tatttgacgg cggccctcgg ctgcggccga tgcagcgggt
     3121 tcgaggtttg ggcggggagg acggctgttg caggaggtct tgtcagtctg gggcgtgagc
     3181 aaagcctatc cgggcgtcac cgcgctcgaa gatgtctcgt tttcgcttcg gcgcggagaa
     3241 attcgagcgc tgtgcggcga gaacggcgcg ggcaagagca cgctcgtcga gattctcatg
     3301 gggctggtcg cgccggatcg cggctcgatc gccgtcaacg gtcaaacgca ggtcatgcgt
     3361 tccccgcagc aggcacaagg cctgggcctc ggcctggtgg cgcaggagct cagcctcgcg
     3421 ccgcatctgt cgatcctcga caatatctgg cttggcagcg ccgaggttcc gcttctgcac
     3481 cggcgagcca agttccgctt gcgcgcgcaa caggccttgg cgacgctcgg ccttgccgac
     3541 tgggatctca atcgcccggt tggctcgctg accatcgctc agcgccaact gatcgagatc
     3601 gcgcgcctgc tggctcgcga cgcccgggtg ctgatcctgg acgagccgac cgcgacgctg
     3661 tccgatgccg acatcgatcg cattctcggc atcctcaggg gtctgcgggc gcaaggccgc
     3721 tccatcatct acgtcacgca tcggctcggc gaggtcttcg agctctgcga ttcagtcacg
     3781 gtgctgcgc
//
LOCUS       DATLJT010000166         2784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1846035,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000166 DATLJT010000000
VERSION     DATLJT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846035"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VM910_03715"
     CDS             complement(<1..470)
                     /locus_tag="VM910_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVI61690.1"
                     /translation="MTQPVAIETLTPAAAPRQPRVGVLLVNLGTPDTADTRGVRVYLK
                     EFLSDPRVIEQQGLFWKLILNGIILRTRPRRKAADYRKIWNTEKNESPLKTITRAQSE
                     KLARSIADHDHVEVDWAMRYGNPSIRAGIESLTARGCDRLLVVPLYPQYSAATSA"
     gene            552..950
                     /locus_tag="VM910_03720"
     CDS             552..950
                     /locus_tag="VM910_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVI61691.1"
                     /translation="MTVAEYCIFACVAIYLLTIVPIKWIGHRQYDNSMPRDPAFYQDG
                     IRARALGAHQNGIEAFPFFAVAVLLAEFRGSPQNLVNELAVLFVIVRIAYVFTYIGNR
                     PRLRSILWGLGFIINVAIFFLPLIRRLLPG"
     gene            complement(957..2006)
                     /locus_tag="VM910_03725"
     CDS             complement(957..2006)
                     /locus_tag="VM910_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HVI61692.1"
                     /translation="MSQARFACGILACTAVLAAVLAADGAIHAIMAQTPFGGPRPPAE
                     PQIGGIVGWLLARQSEFYREISATIRAAKSDGSAVWTLLVISFAYGIFHAAGPGHGKA
                     VISSYLVANQETARRGIVLSFASALMQALVAVLIVSVGAWLLNATAKTMCGAEKAIEI
                     GSYALIAAFGARLVWTKGGSFIRALQASRPEPALAMAAHHHHHGHDRPHAHHDHDGHV
                     HDEHCGHSHGPEPSELAGPRGWRRGLGAVVAVGARPCSGAILVLVFALAQGLFWAGVA
                     ATFVMGLGTAITVATIAVLAVSAKDVARRLSAAGEGGGTLIMRGIEFGAAGLVLLLGS
                     GLLFGYIASERVTCF"
     gene            complement(2006..2647)
                     /locus_tag="VM910_03730"
     CDS             complement(2006..2647)
                     /locus_tag="VM910_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HVI61693.1"
                     /translation="MRATLRRLSVLLPLLSLAGAAEAHPHVWITAVSELLYAPDGSLT
                     GVRHAWTFDDMFASYALQGLETKIKGVYTREELAPLADTNVEALKEYAYFTYARADGG
                     KKEKFSEPVDYFLDYRDGQLTLHFTLPLKTPLKAKQLSLEVFDPTFFVDFQLAKTDPV
                     KLVGAPAGCQMAFQRPNDGTAVAQKLNEQTFMNGGENANYGAMFANKITVDCP"
BASE COUNT          515 a          891 c          881 g          497 t
ORIGIN      
        1 gcagaggtcg cagccgaata ttgcggatag agcggcacca ccagcagacg atcgcaaccg
       61 cgcgccgtca gcgattcgat gccggcgcgg atcgacggat tgccgtagcg catcgcccag
      121 tccacctcga catggtcatg atcggcgatc gaacgcgcga gcttttccga ctgcgcacgg
      181 gtaatggtct tcagcggaga ctcgttcttc tccgtgttcc agatcttccg gtaatcggcc
      241 gcttttcgtc gcgggcgcgt gcgcaggatg atgccgttga gaatcagctt ccagaacagc
      301 ccctgctgct cgatcacccg cggatcggag agaaattcct tcaaatagac gcgcacgccc
      361 ctggtgtcgg cggtatcggg cgtgccgaga ttgaccagga gcacgccgac ccgcggttgc
      421 cgcggcgcgg cggcgggcgt cagggtttcg attgcaaccg gctgcgtcat ggtgttcgtg
      481 gcttcgcgcg cgcgaccatt cttgtcaagc tggccgtgcc ttcgatacga tagccggggc
      541 tgggagggag catgacggtc gccgaatatt gcatcttcgc atgcgtggcg atctatctgt
      601 tgacgatcgt gccgatcaaa tggatcgggc atcgccaata tgacaattcg atgccgcgcg
      661 atcccgcctt ttaccaggat ggcatccgcg cccgcgcact gggcgcgcat cagaacggca
      721 tcgaggcttt tccgtttttc gccgtcgctg tgctgctggc cgaatttcgc ggcagcccgc
      781 agaacctggt caacgagctt gcggtgctgt ttgtgatcgt gcgcatcgca tatgtcttca
      841 cctacatcgg caatcgtccg cggctacggt cgatcctctg gggcctcggc ttcatcatca
      901 atgtcgcgat cttcttcctt ccgctaatcc gacgcctgct gccgggctga tcgtgcttaa
      961 aagcaggtga cgcgctcgga ggcgatgtag ccaaacagga ggccggagcc gagcagaagc
     1021 acgaggccgg cagcgccgaa ttcgatgccg cgcatgatga gcgtgccgcc gccctcgccg
     1081 gccgcgctca gtcgtcgcgc aacatccttg gcggagaccg caagcacggc aatcgtcgcg
     1141 accgtgatcg cagtgccgag ccccatcacg aaggtcgcgg cgacgcccgc ccagaacagg
     1201 ccctgtgcca gcgcaaacac cagcaccaag atcgcgcccg agcacggcct cgcgccgacc
     1261 gcaacgaccg cgcccaatcc ccgccgccag ccgcgagggc cggcgagctc gctcggctcg
     1321 ggtccatggg aatggccgca atgctcgtca tggacatgac catcgtggtc gtggtgggcg
     1381 tgaggacgat cgtgaccgtg atggtgatga tgcgcggcca ttgccagcgc gggctccggc
     1441 cggcttgcct gcagcgcgcg gatgaagctg ccgcctttgg tccagaccag acgcgcaccg
     1501 aatgccgcga tcagcgcgta actaccgatc tcgatcgcct tctcagcgcc gcacatggtc
     1561 tttgcggtgg cattcaacag ccacgcgccg acactgacga tcaggaccgc gaccagcgcc
     1621 tgcatcagcg ccgaggcgaa cgacagcacg atgccacgcc gcgcggtctc ctggttggcg
     1681 acgaggtagg aggagatcac cgccttgccg tggccgggac cagcggcatg aaagattccg
     1741 taggcaaagg agatcacaag cagcgtccag accgcggagc cgtcggactt ggccgcgcgg
     1801 atggtcgcgg atatctcgcg atagaactcc gattgcctgg cgagcagcca cccgacgatg
     1861 ccgccgattt gaggttcggc cggcggcctc ggtcctccga acggcgtctg cgccatgatc
     1921 gcgtggatgg cgccgtcggc tgcgaggaca gcggcgagca cggcggtgca tgccaaaatt
     1981 ccgcacgcaa aacgggcttg cgacatcacg gacagtccac cgtgattttg ttggcgaaca
     2041 tggcaccgta attggcgttc tcgccgccgt tcatgaacgt ctgctcgttg agcttctggg
     2101 ccaccgccgt cccatcattc ggacgctgga aggccatctg gcagccggcg ggcgcgccca
     2161 ccagcttcac cggatcggtc ttggcgagtt ggaagtcgac aaaaaaggtc ggatcaaaga
     2221 cttccagcga aagctgtttc gctttcagcg gggttttcaa cggcaaagtg aaatgcagcg
     2281 tcagttggcc atccctgtag tcgaggaaat aatcgaccgg ctcggaaaac ttctccttct
     2341 tgccgccgtc ggcgcgcgcg taagtaaagt aggcatattc cttcagcgcc tcgacattgg
     2401 tgtcggccaa aggggcgagt tcctcgcgcg tatagactcc cttgatcttg gtctccagac
     2461 cctgcaacgc gtaggaggcg aacatatcgt caaaagtcca ggcgtggcgc acgccggtca
     2521 gcgagccatc gggagcgtag agcaactcgc tcactgcagt gatccagaca tgcgggtgcg
     2581 cctcggccgc gccggcgagc gagagaagcg gcaacaaaac cgaaagcctc cgcaaggtgg
     2641 ctcgcacgtc ggtctcctcg tctcaggcgg tctcgctcaa gcagcagcct cggccgtttc
     2701 caaaaggccg cgccgtcgga gcagcgcgtc cggctccggc tcgcggccgc ggaaggcgac
     2761 ataggcgtct tccggatcga ccga
//
LOCUS       DATLJT010000167         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4143883,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000167 DATLJT010000000
VERSION     DATLJT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143883"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VM910_03735"
     CDS             complement(<1..333)
                     /locus_tag="VM910_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HVI61694.1"
                     /translation="MRQALSVWPRIALLSFGSPAVQLAAMYRILVEERRWISRDRFLN
                     ALNFCLALPGPDAQLLAAYVGWIMHGTLGGMLAGGLVILPPMICMMALSYGYVTGGDS
                     TIGEVLLYG"
     gene            835..1407
                     /locus_tag="VM910_03740"
     CDS             835..1407
                     /locus_tag="VM910_03740"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVI61695.1"
                     /translation="MVEENKDFEQILGGKFDAPYIRKLAAEGAIFERMFAEEHYSQGN
                     YSWLLSGSNQNVGFRDQVPSKANHPDYPFKASNLGEQLIAKGLSFKGYSESLPGIGST
                     VEFDPPNCRGDQCIYARKPEIFAEVQPAVTRYSESHRHSLRRRTYQARLICRGQSHYT
                     REHPAHDRSDVWAAQVERATAQCGGRRHRR"
     gene            1494..2243
                     /locus_tag="VM910_03745"
     CDS             1494..2243
                     /locus_tag="VM910_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HVI61696.1"
                     /translation="MTFGSLVSRLGIPIIALSLTFAQPFAAAHAAENARKVTVVSFGL
                     YGDQGVFRSEATGAAQIVASRFGGGPVVVRFNTKTGGSATVETLAATLQAEAKKMNGE
                     NDILFVILTSHGSPDGLAVTVGRRPAETLKPSKLAEMLERTGVRYKVVIISACYSGVF
                     IPPLANANTLVITAADASHPSFGCQDKAKWTYFGDAFFNVALRQATTLKDAFVVARSL
                     VLKRELRQGFDPSHPQMTGGANVEPLLVARP"
     gene            complement(2313..>2588)
                     /locus_tag="VM910_03750"
     CDS             complement(2313..>2588)
                     /locus_tag="VM910_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61697.1"
                     /translation="VPTSAEVGFAALQGGAWFGLMAPAGTPRATVDWLNAETRKAFEV
                     PEVRERLSKQGLQLPLGSPEEFAVLIAAESKRWGEVIRTGGIKLESN"
BASE COUNT          513 a          801 c          792 g          482 t
ORIGIN      
        1 tccgtaaagc agcacctcgc cgatcgtgga atcaccaccg gtgacatacc catagctcag
       61 cgccatcatg cagatcatgg gcggcaggat gacgaggcca cctgcgagca tgccgccgag
      121 cgtgccgtgc atgatccagc cgacataggc cgcgagcagc tgcgcgtcgg ggccaggtag
      181 cgccaggcaa aagttcaaag cattgagaaa cctgtctcgg gagatccagc gcctttcctc
      241 gacgaggatg cggtacatgg ccgcgagctg cacggcggga cttccgaagc tcagcaaggc
      301 aatgcgcggc cagacgctga gggcctggcg caaggtcgcc ccatcggctc ggctgacggc
      361 aacgttttcg gcggctgttt gcagggagtg tccttcgcag tcacttgcgg gcggtgaacg
      421 gcgcagcgga aagccctccg gcagcggtcc ggatgtgcag ggacgaagtt ttggcgtccc
      481 tcaccacgtg cagccgctcg gcgccgttgc gcccagagct ttcgccaagg tcctgcgatt
      541 gagcatgatc atctccgcgg atcgagtgtc atcaccaaag cgtgggggca gtgatagcac
      601 agccaatgcg gagccggtat cctcgcgcga ttgaatgtgc ttgcatccac tcccgatgga
      661 tccgcagcgc tcgcgacctc gagcgcaagc acaggtctag ctagatggag gacggatgcc
      721 gaaaatttca cacgcgtgcg tcattgcctc ttgtcgtgcg cggccagcac cgcggtggcg
      781 gctagcaggc cgccgtggcc ttcgacactg ccggtctatg accacatcgt cattgtggtc
      841 gaggagaaca aggattttga acaaatcctc gggggcaagt ttgacgcgcc gtatatcagg
      901 aaattggcgg cagagggcgc catattcgag cgcatgtttg ccgaggagca ctatagtcaa
      961 ggcaactatt cctggctgct ctcaggtagc aatcagaacg tcggctttcg cgatcaggtg
     1021 ccgagcaaag ccaaccaccc cgactacccg ttcaaggcca gcaacctcgg cgagcaactg
     1081 atcgccaaag gcctctcgtt caagggatat tcggaaagcc tgcccggcat cggctcgacg
     1141 gtcgagttcg atccgccgaa ctgccgcgga gaccaatgca tttacgcccg caaacccgag
     1201 atattcgccg aggtacaacc agctgttacg cgatattcag aatcgcatcg tcacagtctt
     1261 cgccggagaa catatcaagc caggctcata tgccgagggc aaagccatta cacacgtgaa
     1321 catcctgcgc acgatcgaag cgatgtatgg gctgcccaag tcgagcgcgc aacagcccaa
     1381 tgcggcgggc ggcggcatcg ccgatgagac gatcatgacg gacgtcttcg aggctgtccg
     1441 ttgaggatgt tcagctccgc tgatggtggc gcgcgtgaga acggaggttc gttgtgacgt
     1501 tcggatcttt ggttagccgg ctcggcatac cgatcatcgc cttgtctctg accttcgcgc
     1561 agccgtttgc ggctgcgcac gcggccgaga acgctcgcaa ggtgaccgta gtgtcattcg
     1621 gactgtacgg cgatcagggc gttttccgca gcgaggcgac cggtgcggca cagatcgtgg
     1681 cgagccgatt cgggggcggc ccggttgtcg ttagattcaa taccaagacg ggcgggagcg
     1741 cgaccgtcga aacgcttgca gcaacattgc aagcggaggc aaaaaagatg aatggcgaga
     1801 acgacattct gtttgtcatc ctcacgtcgc acggctcgcc cgacggcctc gccgtcaccg
     1861 ttgggcggcg accggctgaa acgctcaagc cctccaaact cgccgagatg ctcgagcgaa
     1921 ccggcgtgcg atacaaggtc gtgatcattt ccgcttgcta ttctggcgtc ttcatacctc
     1981 ccctcgccaa tgccaatacg ctcgtgatca ccgcggcaga cgccagccat ccatcgttcg
     2041 ggtgtcagga caaagccaag tggacctatt tcggcgatgc gtttttcaat gtcgcgctgc
     2101 gccaggccac gactctgaaa gacgcctttg ttgttgcgcg ctcgctggtc ttgaagcgag
     2161 aactgcgcca gggctttgac ccatcgcacc cgcaaatgac gggaggcgca aacgtggagc
     2221 ccctgctcgt tgcccgcccg tgacgtccag aatttcgatc tcgtgggcgc tgcggtgccc
     2281 tgcaagcgcc atcgcaattg caagcttgcc gctcaattgc tctcgagttt gatgccgcct
     2341 gtgcggatga cctcacccca gcgcttgctc tcggcagcaa tcaacacggc gaactcttcc
     2401 gggcttccga gcggcagctg cagcccctgt ttgctcaagc gctcacgcac ctccggcact
     2461 tcgaacgcct tgcgggtctc ggcgttgagc caatcgacgg tcgcgcgcgg cgtgccggcg
     2521 ggtgccatga gcccgaacca ggcgccgcct tggagcgctg cgaacccgac ttccgccgag
     2581 gtcgggac
//
LOCUS       DATLJT010000168         6710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2076171,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000168 DATLJT010000000
VERSION     DATLJT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076171"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2430
                     /locus_tag="VM910_03755"
     CDS             73..2430
                     /locus_tag="VM910_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVI61698.1"
                     /translation="MTSAETSAASFDPSLAEPKGWSLRRWLAPFAVGIALLSAFLTFV
                     VLTGLTPIEPTREVVRSFLLINAATILLLVGIILREIWQMVQARRRGRAAARLHVQIV
                     SLFSVIAVLPAVLVAIVANVTIERGLDRLFSGPTREVIQNSLIIARAYTYEHAQLIRG
                     DILGMANDIAHARPLFDQDRASFRELLTASAASRNLPGAMLIDKDRNVLETAQTGIQQ
                     NFTTPPPEFLSNVDETDPQIAVFPEANYVAAVIRLKAFSDTFLYVARLLDPRVVAQLK
                     QTETSVAEYAEIESRRLGIQVAFALMFAVIALTILMASVLIGLNFANWLVAPIRRLMN
                     AASDVSTGNLNVQVPVHKSEGDLAQLGETFNKMTQELRTQRDELVNASETIDSRRRFI
                     EAVLSSASAGIIGVDASGSVGILNRSAEKLIGHAESETLDHPLSDVLPELDDMMRTAR
                     EGTQRLVQGQVTILRDGIERNLSVRVSAEKTGQPSDSYIITLDDITELVTAQRTSAWG
                     DVARRIAHEIKNPLTPIQLSAERIRRKFGKVITEDKGVFDQCTETIVRQVDDIRRMVD
                     EFSRFARMPKPVIEGEDVADTVRQAVFLMKVAHPEIDIVVDLKEDPLRAQFDRRLISQ
                     ALTNIIKNATEAIEQVPPDELGKGRIDVVAGRDKDDIVIDVIDNGIGLPKVARARLLE
                     PYVTTREKGTGLGLAIVGRVLEDHGGRIELKDASDFRPGQRGAWMRLRFAVSGRSQSK
                     IEVKEGQLAADAKKAAAETIKPDAETSGPAHATKVEPKIEAATGN"
     gene            2449..3819
                     /locus_tag="VM910_03760"
     CDS             2449..3819
                     /locus_tag="VM910_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVI61699.1"
                     /translation="MASDILIVDDEADIRELVAGILDDEGFSTRTARDSDSALAEVAN
                     RRPHLVFLDIWLQGSKLDGLQLLEQIKKDHPDLPIVMISGHGNIETAVAAIKRGAYDF
                     IEKPFKSDRLILVATRALETSRLKREVRELKQLAPTASTLTGQSPCMNQLRQTIERAA
                     KANSRILIVGPSGAGKELVARTLHQASGRAEGPFVVINAAAITPERMEEELFGVEQSN
                     GEQQRKIGALEEAHGGTLFIDEIADMPRETQNKILRVLVDQTFQRAGGTTKVHVDVRI
                     ISSTGRNLEEEIAAGRFREDLYHRLSVVPIRVPPLSERREDIPQLIDYFMEQISATTG
                     LPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMV
                     PAMPTSNNGEHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKL
                     KALGVG"
     gene            4051..4299
                     /gene="hfq"
                     /locus_tag="VM910_03765"
     CDS             4051..4299
                     /gene="hfq"
                     /locus_tag="VM910_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HVI61700.1"
                     /translation="MAADRAQNLQDTFLNHVRKTKTPLTIFLVNGVKLQGIVTWFDNF
                     CLLLRRDGHSQLVYKHAISTIMPGAPIQLFEGGEDAPA"
     gene            4311..5687
                     /gene="hflX"
                     /locus_tag="VM910_03770"
     CDS             4311..5687
                     /gene="hflX"
                     /locus_tag="VM910_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HVI61701.1"
                     /translation="MEPRNWEGGADRPRSAGARQSGRVIVIGPYVRMRRGSEAQTDEH
                     ALRDFEARLDEAKGLAGAIDLVVADALIAPIAQIRPATYLGKGKVEEITGLVAGDDIE
                     LVVMDCALSPIQQRNLEKAWNTKVLDRTGLILEIFGRRAKTKEGALQVELAHLNYQRS
                     RLVRSWTHLERQRGGFGFMGGPGETQIEADRRQIGDRITRLENELKKVQATRRLHREG
                     RKRVPYRVVALVGYTNAGKSTLFNRLTQSEVQAADMLFATLDPTLRALHLPHGGKAML
                     SDTVGFISNLPTQLVAAFRATLEEVLEADVILHVRDISHEDADPQQRDVDAVLRQLGI
                     DPEAGGRILEVWNKIDRLSPEERANFRKIAARRPPEHPCFLVSAASGEGIDTLLTAIE
                     DRLASSRTTLALSIDASDGAGISWLHRNAEVLDKKLHDGRFDMTVRVDETKRDIVVNR
                     FDAVPHGM"
     gene            complement(5712..6689)
                     /locus_tag="VM910_03775"
     CDS             complement(5712..6689)
                     /locus_tag="VM910_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61702.1"
                     /translation="MKLPRRQFLSLATIALGAPALSRVANAQAYPTRPVRMLVGFAAG
                     GAPDIAARLIAQWLSERLGQQFVVENRPGAGGNIATEAVVDAPADGYTLLMAGLQNAV
                     NTTLYQKLKFDFARDMAPVAGVSHENYGMEVHPSFPAKTVPEFIAYAKANPGKLNMAS
                     PGNGTGPHMAGELFKIMAGVDMVHVPYRGSPPALTDLLAGQVQLMFSPLSSSIEYVRA
                     GKLRGLAVTSATRSDVLPDLPTVGEYVAGYQATGWFGVCVPRKTPAEIVDRLNREINA
                     GLADSKIKARFADLGATVFVGSPSDFAKHIADETEKWGKVVRMANLRAD"
BASE COUNT         1304 a         2095 c         2146 g         1165 t
ORIGIN      
        1 tcggcaacat tgtgatatga atgcatcagt ccgcatgcgg gcgaagccat tcagcatcac
       61 actatttccg gaatgaccag cgcagagacc tcggccgcct cgttcgatcc gtcgcttgcc
      121 gaacccaagg gatggtcctt gaggcggtgg cttgcgccgt tcgccgtcgg catcgcgctg
      181 ctgtcggcgt tcctcacctt cgtggtgctg accggcctga cgccgatcga gccgacgcgc
      241 gaggtggtcc ggtccttcct gctgatcaac gcagcgacca tcctgctgct ggtcggcatc
      301 atcctgcgcg aaatctggca gatggtgcag gcgcggcgcc ggggaagggc tgcggcgcgg
      361 ctgcatgtgc agatcgtcag cctgttctcg gtcattgccg tgctgccggc agtgctggtc
      421 gcgatcgtcg ccaacgtcac catcgaacgc ggtctggacc ggctgttctc gggtccgacg
      481 cgggaggtga ttcagaactc gctgatcatc gcgcgtgcct atacctacga gcatgcccag
      541 ctcatccgcg gcgacattct gggcatggcc aacgatatcg cccatgcccg tccgctgttc
      601 gatcaggacc gcgccagctt tcgcgaattg ctgaccgcga gcgcggcctc gcgtaacctg
      661 ccgggcgcca tgttgatcga caaggaccgc aacgtgctgg agacggcgca gaccggcatc
      721 cagcagaact tcacgacccc gccacccgaa tttctgagca acgtcgacga aaccgatccc
      781 cagatcgccg tgttcccgga agccaattat gtcgcggcgg tgatccggtt gaaagccttc
      841 agcgacacct tcctctacgt cgcgcggctg ctcgatccgc gcgtggtggc gcaactcaag
      901 cagaccgaga ccagtgttgc cgaatatgcg gagatcgaat ccagaaggct cggcattcag
      961 gtggcgttcg cgctgatgtt cgcggtgatc gcgctgacca tcctgatggc gtcggtgctg
     1021 atcgggctga atttcgcgaa ctggctggtc gcgccgatcc gccgcctgat gaatgcggca
     1081 agcgacgtct cgaccggcaa tctgaacgtg caggtgcccg tgcacaaatc cgagggcgac
     1141 cttgcgcaac tcggcgagac cttcaacaag atgacgcagg agctgcgcac ccagcgcgac
     1201 gaactcgtca acgccagcga gaccatcgac agccgccgtc gcttcatcga ggcggtgttg
     1261 tcttccgcca gcgccgggat catcggcgtc gatgcctcgg gcagcgtcgg catcctcaat
     1321 cgttcggccg agaagctgat cgggcacgcg gaatccgaga cgctcgatca tcccctgtcc
     1381 gacgtgctgc ccgagctcga cgacatgatg aggaccgcgc gcgagggcac gcagcggctg
     1441 gtgcagggcc aggtcacgat tttgcgcgac ggcatcgagc gcaatctctc ggtgcgcgtc
     1501 agcgccgaaa aaaccgggca gccgagcgac agctacatca tcacgctcga cgacatcacc
     1561 gaactcgtga ccgcacagcg cacctcggcc tggggcgatg tcgcccgccg catcgcccac
     1621 gaaatcaaga atccgctgac gccgattcag ctatccgccg aacgaatccg ccgcaaattc
     1681 ggcaaggtca tcaccgagga caagggggtg ttcgaccaat gcacggaaac catcgtgcgc
     1741 caggtcgacg acatcaggcg gatggtcgat gagttctcac gctttgcgcg catgccaaaa
     1801 cccgtcatcg aaggcgagga tgtcgccgac acggtgcggc aggccgtgtt cctgatgaag
     1861 gtcgcgcatc ccgagatcga tatcgttgtt gatctgaagg aagacccgct gcgggcgcaa
     1921 ttcgaccgcc ggctgatctc gcaggcgctg accaacatca tcaagaacgc gacagaagcc
     1981 atcgagcagg tgccgccgga tgagctcgga aagggtcgta tcgacgtcgt tgccgggcgc
     2041 gacaaggacg acatcgtgat cgacgtgatc gacaacggta taggattgcc gaaggtggcg
     2101 cgagcgcgcc tgctcgaacc ctatgtcacg acgcgcgaaa agggcacggg cctgggactc
     2161 gccattgtcg gccgcgtgct ggaagaccat ggcggccgaa tcgagctgaa ggacgcctcg
     2221 gatttccgcc ccggccagcg cggcgcctgg atgcgactgc gctttgcggt ttccgggcgc
     2281 agccaatcca agatcgaggt gaaggaaggg caactggccg ccgacgccaa gaaggcggcg
     2341 gccgaaacaa ttaagccgga cgcagaaacc agcgggccgg ctcacgcgac taaggttgaa
     2401 ccaaaaatcg aagccgctac aggcaactga cgaggcgggg cgtgacccat ggcaagtgac
     2461 attctgatcg tcgacgatga ggccgatatt cgggaactcg ttgcaggcat tcttgacgac
     2521 gaggggtttt cgaccaggac ggcgcgcgac agcgattccg cgctggcgga ggtcgccaat
     2581 cggcggccgc atcttgtctt tctcgatatc tggctgcagg gctcgaagct cgacggcctg
     2641 caacttctcg agcaaatcaa gaaggatcat cccgatctgc cgatcgtgat gatctcgggt
     2701 cacggcaaca tcgagaccgc cgtcgcggcg atcaagcgcg gcgcctatga tttcatcgag
     2761 aagccgttca agtccgaccg cctcatcctg gtcgcaacgc gggcgctcga gacctcgcgg
     2821 ttgaaacggg aggtgaggga actcaagcaa ctcgcgccca ccgcgagcac gctcaccggc
     2881 cagtcgccct gcatgaacca gttgcggcag accatcgaac gtgccgcgaa agccaacagc
     2941 cgcatcctga tcgtcggccc ctccggcgcg ggcaaggagc tggtggcgcg tacgctgcat
     3001 caggcttccg gccgcgccga ggggcctttc gtcgtgatca acgccgcggc gatcacgccg
     3061 gagcggatgg aggaggagct gttcggggtc gagcagtcca acggcgagca gcagcgcaag
     3121 atcggcgcgc tcgaagaagc ccatggcggc acgctgttca tcgacgagat cgccgacatg
     3181 ccacgcgaga cccagaacaa gatcctgcgg gtattggtcg accagacctt tcagcgcgcc
     3241 ggcggcacga ccaaggtcca tgtcgatgtc cgcatcattt cctccaccgg gcgcaacctg
     3301 gaagaggaga tcgcagccgg gagattccgt gaggacctct atcaccggct gtcggtggtg
     3361 ccgatccgcg tgccgccgct gtcggaacgg cgcgaggata ttccgcagct aatagattat
     3421 ttcatggagc agatttcggc gaccaccggc ctgccgaaac gtcagatcgg gcaggacgcg
     3481 atggcagtgc tgcagtccca tgtctggccg ggcaacgtgc ggcagctccg caacaatgtc
     3541 gagcgcgtca tgatcctggc gggcggcggg cccgaggtca tcattaccgc cgacatgctg
     3601 ccacaggacg ttggctcgat ggtgccggca atgccgacct ccaacaacgg cgagcacatc
     3661 atgggcctgc cgctccggga ggcccgcgag gtattcgagc gcgactatct gatcgcgcag
     3721 atcagccgct tctcgggcaa catctcgcgc acggcggagt tcgtcggaat ggagcgctcc
     3781 gccctgcacc gcaagctgaa ggcgctcggc gtcgggtaac cgcgttatct tggaattttc
     3841 gtgagctttc ggggcttttg ccccgaattg aggctttcgg ctgccggaag ggcttgcgaa
     3901 gcggcttgcc taaaaagcgt acctgccttc taattgtacc gtcgcgtgtc ccccgagggg
     3961 gatctcatgg gataccggca accgggtcag gctccgtcag agggcgaaaa cccggttcaa
     4021 taaaaagaag cacctagcgg gataacaaca atggcggcag accgcgcaca aaacctacaa
     4081 gacaccttcc ttaatcacgt tcgcaaaacc aaaacgccac tgacgatctt tctggtcaat
     4141 ggggtgaagc tgcaggggat tgtcacctgg ttcgacaatt tctgtctgct gctccggcgc
     4201 gacggtcatt cgcagcttgt ctacaagcac gcgatctcga ccatcatgcc gggcgctccg
     4261 atccagttgt tcgagggcgg cgaggatgcg ccggcttgaa agtgaattga ttggaacccc
     4321 ggaattggga agggggagct gaccgtccga ggtcggcagg ggcacgtcaa agcgggcggg
     4381 tgattgtcat cggtccgtat gtgcgaatgc gccgcggcag cgaggcgcaa acggacgagc
     4441 atgccttgcg cgatttcgag gcgcggctgg acgaggccaa ggggctggcg ggcgcaatcg
     4501 atctcgtcgt tgccgacgcg ctgattgcgc cgatcgccca aatccgtcct gcgacctacc
     4561 tcggcaaggg caaggtcgag gagatcacgg gcctcgttgc cggtgatgac atcgagctcg
     4621 tggtgatgga ctgcgcgctg tcgccgatcc agcaacgcaa cctcgagaag gcctggaaca
     4681 ccaaggtgct cgaccgcacc gggctgatcc tggaaatctt cggccgccgc gccaagacga
     4741 aagagggcgc gctgcaggtc gaactcgcgc atctcaacta tcagcgcagc cgcctggtaa
     4801 gatcctggac ccacctcgaa cggcagcgcg gcggttttgg gttcatgggt ggccccggcg
     4861 aaacccagat cgaagccgac cgccgccaga tcggcgatcg catcacccgg cttgagaatg
     4921 agctgaaaaa ggttcaggcg acccggcggc tgcatcgcga ggggcgcaag cgcgtgccgt
     4981 atcgggtcgt ggcgctggtc ggctacacca acgccggcaa gtcgaccttg ttcaaccggc
     5041 tgacgcaatc ggaggtgcag gccgccgaca tgctgttcgc gacgcttgac ccgactttgc
     5101 gggcgctgca tctgccgcac ggcggcaagg ccatgttgtc cgataccgtc ggcttcatct
     5161 ccaatctgcc gacacaactg gttgcggcct tccgcgcgac gctcgaagag gtgctcgagg
     5221 ccgacgtgat cctccacgtc cgcgacatct cgcatgagga cgcagacccc cagcagcgtg
     5281 atgtcgatgc cgtgctgcgc cagcttggca tcgacccgga ggcaggcggc cgcatcctcg
     5341 aagtctggaa caagatcgat cgcctctcac cggaggagcg cgcgaatttt cgcaagatcg
     5401 ccgcccgccg cccgcccgag catccctgtt tcctggtctc tgccgcgagc ggggagggga
     5461 tcgatacgct cttgaccgcg atcgaggaca ggctggcatc ctcgcggacg acgctcgctc
     5521 tgtcgatcga tgcttccgac ggcgccggca tctcgtggct gcatcgcaat gccgaggtgc
     5581 tggacaagaa gctgcacgac ggccgcttcg acatgactgt gcgcgtcgac gagaccaagc
     5641 gcgacatcgt ggtcaaccgc ttcgacgccg tgccgcacgg gatgtaggca agttgcgcct
     5701 tggcgatccc gtcaatccgc cctgagattg gccatccgaa ccactttgcc ccacttctcc
     5761 gtttcgtctg cgatatgctt tgcgaaatcg gagggcgaac cgacaaacac ggtggcgccg
     5821 agatcggcaa accgtgcctt gatcttcgaa tccgcaagtc ccgcattgat ctcccggttt
     5881 agccggtcga cgatctcggc cggcgttttc cggggcacgc agacgccgaa ccaacctgtc
     5941 gcctgatagc cggctacgta ttccccgacg gttggaagat cgggcagcac atccgagcgc
     6001 gtcgccgagg tcaccgccag gccacgaagc ttaccggctc tgacatactc gatcgacgaa
     6061 gacagcgggc taaacatcaa ctgcacttgt ccggcaagca gatcggtcaa tgcgggcggt
     6121 gagccgcgat agggcacgtg caccatatcg acgccggcca tgatcttgaa cagctcgccg
     6181 gccatatgag gtcctgtgcc gttgccgggc gacgccatgt tgagcttgcc tgggttggcc
     6241 ttggcgtagg cgatgaattc agggaccgtc tttgccggaa aggacgggtg cacctccatg
     6301 ccgtagttct cgtggctgac accggcaacc ggcgccatgt cgcgggcaaa atcgaatttg
     6361 agcttttggt agagcgtcgt gttgacggcg ttttgcaggc cggccatcag aagcgtatag
     6421 ccatcggcag gtgcgtccac cacggcctcg gtggcgatgt tgccgccggc gccgggccgg
     6481 ttctcaacga cgaattgctg accaagccgt tcggacagcc attgcgcgat caatcgcgct
     6541 gcgatgtcgg gggcgccgcc ggcggcgaaa ccgacgagca tccgcacggg ccgcgtcgga
     6601 taggcttgcg catttgccac gcgcgagagg gcgggagcgc caagtgcgat tgtggcgagg
     6661 gacaggaact ggcggcgagg aagtttcatc gcagtcctgc gtgtgggtgt
//
LOCUS       DATLJT010000169         4546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_927317,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000169 DATLJT010000000
VERSION     DATLJT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927317"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VM910_03780"
     CDS             complement(<1..315)
                     /locus_tag="VM910_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61703.1"
                     /translation="MVGADAFFNANSRLLAEATLRHAVATIYEYTEFTDAGGLMSYGG
                     NINESYRWAGIYAGRILKGAKPADLPVQQSTTVELIVNLKTAKALGITVPLSLLGRAD
                     KII"
     gene            complement(348..968)
                     /locus_tag="VM910_03785"
     CDS             complement(348..968)
                     /locus_tag="VM910_03785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61704.1"
                     /translation="MRRREFITLLGGTVLSPFAAHAQQNLPTIGFLNGTSAEKYAPYI
                     ASFYAGLKEGGFVEGENVAIEYRWADGDYSRLPALAADLVKRQVAVIVANTPANLAAK
                     KATDSIPVVFTTASDPVQIGLVDNISRPGGNVTGISQLNASLAPKRLELAHELMPNAT
                     DIALLVNPNDPARAEKISAEVQEAADHLGLHLHLLRAGTERISRRL"
     gene            complement(1105..2208)
                     /locus_tag="VM910_03790"
     CDS             complement(1105..2208)
                     /locus_tag="VM910_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HVI61705.1"
                     /translation="MNMHLTKSQIGLDGVPAAETVLSHVDGERGELIIAGEHVADLAG
                     KSSFESVTARLWNGATGRPLSEANVRASLGGARQRAFDRLAALLPATRGLSIVDGFRA
                     AIAGLRGESGIDHEATVVGAFPVIAGALVQQAKGHDPVAPDPNISHAADTLKMLRGRK
                     ADAREIAALDAYLVTVCDHGMNASTFTTRVVASTQADLFAAVTAGYCALTGPLHGGAP
                     EPVLEMLDAIGTRERIKPWVDDALARGERLMGFGHRIYRVRDPRADVLKAAIERLAAD
                     GADLPFASEVEAYIRQALRRKNPDRPLETNVEFFTAILLDALAIPRQAFTPTFAVARA
                     VGWTAHAREQQRTGRLIRPSSSYVGPIPGVARG"
     gene            2283..3503
                     /locus_tag="VM910_03795"
     CDS             2283..3503
                     /locus_tag="VM910_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HVI61706.1"
                     /translation="MKKSDGLYLSAREASAELAISPATLYAYVSRGLIRSEPTPDSRS
                     HRYLAEDVRELKERRTPSPEPRGFRSFDPDLPVMDSAISTITEAGPIYRGVNCVELAE
                     KDTLEHTATLLWDVTRVDPFSADNCPQMSEEMRAIAEAARRAAPIDRAIAVLALATSA
                     DRGAFTRAPDGRAMVGGRIMRLLVATMLNTPPSAEPLHLQIARAWAPDHKHAADLIRR
                     ALVLLADHELNASTFTARCAASTGLNLYDSVIAGLAALKGPKHGGAGVLASQLVKTLI
                     SNDAEPVIRERVALGERFAGFGHGVYKRGDPRAISLLEALTRAGAARKFTKEVPQRIA
                     EATGEFVNIDYALAVLAHSLRFAAGSELTLFAMARAVGWIAHASEQLQHGRLIRPRAR
                     YVGPPARGVASQQD"
     gene            complement(3510..4172)
                     /locus_tag="VM910_03800"
     CDS             complement(3510..4172)
                     /locus_tag="VM910_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVI61707.1"
                     /translation="MAAYIDPLIAFVSDNAWLAYLTLFLAALLEAVPVIGSVIPGSTI
                     ILALSALVPGGELKLHWVLLAATVGAMIGDGSAFWVGHRAKNEILSTWPLTNYPRVVA
                     QSEAFFRHRGTWAVFFARFVPPIRAFVPITAGALGMPPPRFYAVNIPAILLWAPLHVL
                     PGVMAVSVLDRIGVFKHTGGSAKHHWMPIVLGGALILALAVWTIRRRNGGGIIEPAEP
                     AK"
BASE COUNT          839 a         1443 c         1468 g          796 t
ORIGIN      
        1 gatgatcttg tcggcgcggc cgagcaacga cagcggtacg gtgatgccga gggccttcgc
       61 ggttttcagg ttcacgatca attcgacggt ggtggactgc tgcaccggca gatcggcggg
      121 ttttgcgccc ttcagaatcc gtcccgcata gatgccggcc cagcggtagg attcattgat
      181 gttgccgccg tagctcatca ggccgccggc gtcggtgaac tcggtgtatt catagatcgt
      241 ggcaactgca tgccggagcg tggcttccgc caacagccgg ctgttggcgt tgaagaaggc
      301 atcggctccg accacgagta cgctggcctt gagtcgggag aaatcggcta cagccttctc
      361 gatatccgtt cggtaccggc gcgcagaagg tgaagatgca atccgagatg gtcggccgct
      421 tcctggacct cagccgaaat cttttcggcg cgggcggggt cgttcgggtt gacgagcagt
      481 gcgatgtccg tcgcattcgg catcagttca tgtgccagct ccagccgctt cggggcgagc
      541 gatgcgttca actggctgat gccggtgaca ttgccgccgg gccggctaat gttgtcgaca
      601 aggccgatct ggactgggtc gcttgccgta gtgaacacca ccggaatcga gtcggtcgcc
      661 ttcttcgcag cgagattggc gggcgtgttg gcgacgatca ccgcgacctg acgtttcacg
      721 agatcggcgg cgagcgcagg caggcgacta taatcaccgt cggcccatcg gtactcgatt
      781 gcgacgtttt cgccttcaac aaatccgccc tctttcagcc cggcgtagaa tgacgcgata
      841 tagggcgcgt atttttccgc agacgtgcca ttgagaaatc cgatggtggg caggttctgc
      901 tgagcatgcg ccgcaaacgg cgacaatacg gtgccgccga gtagcgtgat aaactcccgg
      961 cgtcgcatgg cttgccgatc tccgcccctt ccggagatgt cattagccgc aattgcggaa
     1021 ccccgcacgg cacaggatgt cgcaggccga tgtccgaggg ccgtggtgcg cgcgagtcgg
     1081 cgacgcgcgc acaaccccgg tcactcatcc gcgcgccacg ccaggaatcg gtccgacata
     1141 ggatgaactc ggacggatca gccggccggt gcgctgttgt tcgcgcgcat gggcggtcca
     1201 gcctactgcg cgggcaaccg caaaggtcgg cgtgaaggcc tggcgcggaa tcgccagcgc
     1261 atcgagcagg atcgcagtga agaactcgac attggtctcc agcgggcggt ccggattctt
     1321 tcgacgcagc gcctgacgga tataggcttc gacctcgctc gcgaagggaa ggtcggcgcc
     1381 gtcggccgca agccgctcga tcgccgcctt caggacgtcg gcacgagggt cgcgcacgcg
     1441 atagatgcga tgcccaaatc ccatcaggcg ctcgccgcgc gccagcgcat cgtcgaccca
     1501 tggcttgatg cgttcgcgcg tgccgatggc atcgagcatc tccagcaccg gctcgggggc
     1561 accgccgtgc agcggtccgg tcagggcgca atagccggcc gtgaccgcag caaaaaggtc
     1621 ggcctgggtc gacgcgacga cgcgtgtcgt gaaggtggag gcattcatgc cgtgatcgca
     1681 cacggtgacg agataggcgt ccagcgcggc gatctctcgc gcgtccgcct tgcggccgcg
     1741 gagcattttc agcgtgtcgg cggcatgact gatgttcgga tctggggcga ctggatcgtg
     1801 gccctttgcc tgctgcacca gtgcgccggc gatcacagga aacgcgccga cgacggttgc
     1861 ctcatgatcg atcccgctct cgccgcgcaa acctgcgatt gcggcgcgaa agccgtcgac
     1921 gatcgatagt ccacgcgtcg ccggcaacag agcggcgagt cggtcaaaag cgcgttgccg
     1981 tgcgccgccg aggcttgcgc ggacattggc ctcgctgagt ggtcgtcccg tcgcaccatt
     2041 ccagaggcgc gcggtcacgc tttcaaagct cgatttgcct gcaagatcgg cgacgtgttc
     2101 gccggcgatg atcagttcgc cacgttcgcc gtcgacatgg ctcaacacgg tctcggcggc
     2161 ggggacgccg tccagcccga tctggctttt ggtgagatgc atgttcatgg ccgaatctcc
     2221 tttccgactt ggaaagatcg gccgtctcga catattgatc aatcttgatt atataaatca
     2281 atatgaaaaa atccgatggt ctttacctct cggcccggga ggcctcggcc gagctcgcga
     2341 tctcgccggc gacgctctac gcctatgtca gccgcgggct gattcgctcc gagccgacgc
     2401 cggactcccg cagccatcgc tacctcgctg aagacgtcag ggagctcaaa gagcgtcgga
     2461 cgccttcccc ggagccgcgc ggattcagga gctttgaccc tgatctgccg gtgatggatt
     2521 cggccatttc gacgatcacc gaggctggcc cgatctaccg cggcgtcaac tgcgtcgagc
     2581 tggccgaaaa ggacacgctc gaacacacag cgaccctgct gtgggacgtg acccgggtcg
     2641 atccgttttc cgccgataac tgcccgcaaa tgtcggagga gatgcgcgcg atcgcggaag
     2701 ctgcacgccg tgcggcgccg atcgatcgtg caatcgcggt gcttgcactt gcgaccagcg
     2761 ccgatcgcgg cgccttcacc cgcgcccccg acggtcgcgc gatggtaggc ggtcgcatca
     2821 tgcggctgct ggttgcgacc atgctcaaca ccccaccatc agccgagccg ctgcatctgc
     2881 agatcgccag agcctgggcg ccggatcaca aacatgccgc cgatctgatc cggcgcgcgc
     2941 tggtgctgct cgccgatcac gagctgaatg cctcgacctt cacggcgcgt tgcgcggctt
     3001 cgaccggcct gaacctctat gattccgtga tcgcaggact tgccgcgctg aaggggccca
     3061 agcacggcgg tgcgggtgtg ctggcctcgc aactggtgaa gacgctgatc tccaatgacg
     3121 cggagccggt aatccgggag cgcgtcgcgc tgggcgagcg tttcgccggc ttcggccatg
     3181 gcgtctacaa gcgcggcgat ccccgcgcga tttcgctgct cgaggcgctg acgcgagccg
     3241 gcgcggcacg gaaattcacg aaagaagtcc cgcagcggat cgccgaagcc accggcgagt
     3301 ttgtcaacat cgactatgcc cttgccgtgc tcgcacacag cctgcggttc gcggctggaa
     3361 gcgaactcac cctgtttgcc atggcccgtg ccgtcgggtg gattgcgcat gcgagcgagc
     3421 aattgcagca cggccggctg atccgcccgc gcgcacgcta tgtggggccg ccggcgcgcg
     3481 gcgtcgcctc gcaacaggat tgaatggact cacttggccg gctccgccgg ctcgatgatg
     3541 ccaccgccgt tgcgccgccg aatggtccag accgcgagcg cgagaatcaa cgcgccgccg
     3601 agcacgatcg gcatccagtg atgtttggcg ctgccgccgg tatgtttgaa cacgccaatg
     3661 cgatcgagca ccgagacggc catcaccccg ggcagcacgt gcaagggcgc ccataacagg
     3721 atcgccggga tgttgaccgc gtagaagcgc ggcggaggca tccccagcgc gccggccgtg
     3781 atcggcacga aggcgcggat cggcggcacg aaacgggcaa agaataccgc ccaggtgccc
     3841 cggtgccgga agaaggcttc gctctgggca accacgcgcg gataattggt caggggccag
     3901 gtcgaaagga tctcgttttt ggcgcggtgc cccacccaga acgccgatcc gtcgccgatc
     3961 atcgcgccga cggtcgcagc cagcagcacc caatgcagct tcaactcgcc gccgggcacg
     4021 agcgcgctca gcgcaaggat gatggtcgag ccgggaatca ccgatccgat gaccggaacg
     4081 gcctccagca gcgcggcaag gaacagcgtg agataggcca gccaggcgtt atcagagaca
     4141 aaggcgatca atggatcaat gtaagcggcc acaaaatccc tgtattcgtt gagtgcccgg
     4201 cgccgaacga cctagcataa ctaggccggc gacgggaaag tgccatggga acccgcctct
     4261 gcgccggcgg cgaacagaaa tgccgcgcaa ttcgcagggc agccttccaa aaaccgcgtc
     4321 ccgtgcctat ctcgatgaaa aagcaacgga actttgatca agcggcgggc ttctgccggc
     4381 tcttgctctc tgataggctg acaataagcc cccaaccgca gccatatcgc taatctggtt
     4441 tcgggaatgc ccgggacccc ggaggattga tgacgaccgt cgccaccaca acagcgcagg
     4501 aaatgaccgg gttgccggat atgaaggtgt cggtccggca ggtctt
//
LOCUS       DATLJT010000170         3272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3454654,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000170 DATLJT010000000
VERSION     DATLJT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454654"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VM910_03805"
     CDS             <1..1209
                     /locus_tag="VM910_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI61708.1"
                     /translation="CESMPVLVVSGNELNRMILIDQLSARAVRSGQAQTGSEALEALR
                     AAALRGDPYRRAVIDNSLPDMSGIELAHAIKSISAHADLQLVLLTPAAQDVGESGGGV
                     VRCLPKPIRQSAFWHCFTSGCAEVPTTTQNATDTPTSKSQGIKGARVLLVEDEPVNVE
                     VCVAMLESMGCTVETAINGLCALDRHACGEFGLILMDCQMPEMDGYQATAEIRRREAL
                     SNRRTPIVAVTGIGARERCLAAGMDDYLAKPYTLDQIRAMLTTWLSPSVPPVKRDHLA
                     LVPASSPLNEPLDYKVLDSLRQLQREGRPDIVQQVINLFFNDAEDLLRDLTEGAENKD
                     TELLHQASHALKSVSANVGAIILSSRCGELEVMAQAGTVAGAGGMVSAIIEAYRAVEI
                     LLSARLPKVA"
     gene            1371..1814
                     /locus_tag="VM910_03810"
     CDS             1371..1814
                     /locus_tag="VM910_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI61709.1"
                     /translation="MNAYFKVISLVERLHRQFLELVKLELEGLGIHDINNVQGMMLFS
                     IGDAEMTVGELTLRGCYLGSNVSYNVKKMVENGYLMQERSLHDRRSIHVHLTEKGCEL
                     RDSLTAMHQRHLEMLSQAELTADDLQSVSVTLRRLEQFWIRVAGP"
     gene            complement(1953..3023)
                     /gene="recA"
                     /locus_tag="VM910_03815"
     CDS             complement(1953..3023)
                     /gene="recA"
                     /locus_tag="VM910_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVI61710.1"
                     /translation="MMQTALRLLDKEAMDKQRALDAALGQIERAFGKGSIMKLGSHEA
                     AAETEVISTGSLGLDIALGIGGLPRGRVIEIYGPESSGKTTLALHVIAEGQRGGGTCA
                     FIDAEHALDPGYARKLGVNVDELLISQPDAGEQALEIADTLVRSGAIDVLVIDSVAAL
                     VPRAELEGEMGDSHVGLHARLMSQALRKLTGSIARSRTMVIFINQIRLKIGVMFGNPE
                     TTTGGNALKFYASVRLDIRRIGAIKERDQVIGNQTRVKVVKNKMAPPFKVVDFDIMYG
                     EGVSKRGELIDLGVQAGVVEKSGSWFSYEGQRIGQGRENAKAYLKDHSEVGEAIEHAI
                     RANAGLVAEAMMAAPGDAAAEE"
BASE COUNT          653 a          988 c          987 g          644 t
ORIGIN      
        1 tgcgaaagta tgcccgtcct ggtcgtttcc ggcaacgagc tcaatcgtat gatcttgatc
       61 gatcagctgt cggcccgggc ggtccgcagc ggccaagcgc aaaccggctc ggaagcgctg
      121 gaagcgcttc gcgccgcggc ccttcgcggg gacccgtacc ggcgggccgt tatcgacaat
      181 tcgctgcccg acatgagtgg gatcgagcta gcgcacgcga tcaagtcgat ctccgcgcac
      241 gcagacttgc agctggtgtt gttgacccct gctgctcagg acgtcggcga atctgggggc
      301 ggcgtggtca ggtgcctgcc aaagcctatc cggcagtccg cattttggca ttgtttcacc
      361 tctggctgtg ccgaggttcc cacgacgacg cagaacgcca ccgatactcc gacctcaaaa
      421 agccaaggca tcaaaggagc gcgcgtgctg ttggtggagg acgagccggt caatgtcgag
      481 gtgtgtgtcg ccatgctgga gagcatgggc tgcacagtcg agactgccat taacggttta
      541 tgcgcgctcg accggcacgc ctgcggcgag ttcgggctga tccttatgga ttgccagatg
      601 cccgagatgg atgggtatca ggcgactgca gaaattcgac ggcgcgaagc cctctcgaac
      661 cgtcgaaccc caatcgtcgc cgtgacgggg ataggtgcgc gagagcggtg cctggcagca
      721 gggatggacg actaccttgc gaagccatac accctggatc agataagggc gatgttgaca
      781 acatggctca gcccatctgt cccccctgtc aagcgagacc atttggcact ggtgcccgca
      841 tcgtccccgc ttaacgaacc cctcgactac aaggtactag actcgctccg gcagctccag
      901 agggaggggc gtccggacat tgtgcagcaa gtgataaatt tgtttttcaa tgatgcggaa
      961 gatctcctta gggacttgac cgaaggcgct gagaataagg acactgagct gcttcaccag
     1021 gccagccacg cgctgaagtc ggtcagtgca aatgtgggtg cgatcatact atcgtcgcgt
     1081 tgcggggagc tcgaagtgat ggcacaagcg ggtacggtgg ccggcgctgg gggaatggtc
     1141 agcgcgatca tcgaagccta ccgagcggtg gagatcctgc tgtcggcccg tctccccaaa
     1201 gtggcctgat cgccgatggg tcgccccgcg gacatccgct gatttggtgg tcgattctgt
     1261 tcagaccgcg agcgctgtct atcggctgat ctgcaaaccc gaccgcgaca ggtgacttgt
     1321 cctcaaggcc aacgccgacc agcacgagat cgcgttaaaa cgaggctcga atgaacgctt
     1381 atttcaaagt catttcgctc gtcgagcgcc ttcaccgtca gtttctagaa ctggtaaagc
     1441 tcgagctcga ggggctgggg atacacgaca tcaacaatgt ccagggaatg atgctattta
     1501 gcattggtga tgccgaaatg accgtcggcg aactgacatt gaggggctgc tatctcggtt
     1561 cgaacgtctc ttacaatgtc aagaagatgg tcgagaacgg ctatctcatg caggagcgct
     1621 cgctgcacga ccgccgctct atccacgttc atctcaccga aaaggggtgc gagctgcggg
     1681 acagcctgac agcgatgcat cagcgtcatc tcgagatgct gtcgcaggct gaactcactg
     1741 cggacgatct tcagtctgtc agcgtcacgc tgcgtcggct cgagcagttc tggatccgcg
     1801 ttgccggccc gtaagccgaa cctctgttgc gccgggggct cgccaaggtg agtcgggaac
     1861 cgggatcggt cgcacaactt ctggccgcag cggccttatg cgatgcgaac gcgagaacat
     1921 cgccccgacg gatgttctcg gacggcagta atctattcct cggccgcagc atccccggga
     1981 gcggccatca tcgcttcggc gacgagacca gcattggcgc ggatagcgtg ctcgatcgct
     2041 tcaccgactt cgctatggtc tttcaaatag gccttggcgt tctcgcggcc ctgaccgatt
     2101 cgctgaccct cgtaggagaa ccatgagccg gatttctcga cgacacccgc ctggacgccg
     2161 aggtcgatca actcgccgcg cttcgacacg ccctcgccgt acatgatatc gaaatcgacc
     2221 accttgaatg gcggcgccat cttgttcttg acaaccttga cgcgtgtctg gttgccgatg
     2281 acctggtcac gctccttgat cgcgccgatg cgcctaatgt cgaggcgaac cgaagcgtag
     2341 aactttaacg cgttgccgcc ggtggtggtc tcgggattgc caaacatcac gccgatcttg
     2401 aggcggatct gattgataaa aattaccatt gtgcgagagc gggcgatcga tcccgtcaat
     2461 ttgcgcaatg cctggctcat cagcctggca tgtagaccga catgactgtc gcccatctcg
     2521 ccctcgagct cggcgcgggg aacgagggcc gccaccgaat cgatgaccag gacatctatc
     2581 gcgccggagc ggacgagagt gtcggcgatc tcaagggcct gctcgccggc gtcgggctgc
     2641 gaaatcaaca gttcgtcgac attgaccccg agcttgcgag cgtagcccgg gtcgagggca
     2701 tgctccgcat cgatgaaggc gcaggtccca ccgccgcgct ggccctcggc aatcacgtgc
     2761 aacgcgaggg tggtcttacc ggagctctcc ggtccgtaga tctcaatgac gcggccgcgt
     2821 ggcaatccgc cgataccgag cgcgatatcg aggccgagcg agccggtcga aataacctcg
     2881 gtctccgccg cggcctcgtg cgacccgagt ttcataatcg accccttgcc aaaggcgcgt
     2941 tcgatttggc cgagcgccgc gtccaaagcc cgttgcttgt ccatcgcttc cttgtccaaa
     3001 aggcgcaacg ctgtctgcat catccttcct cccttattcc acagctggtg aggccgtgca
     3061 acggacctgg ggacggtaac gtacacgatt tgttcaggga tgcaattgag gaatggacca
     3121 gacaaaaata aagatccgca gccccgctcc gcacgtagca tacttggcag ttcgcgcctc
     3181 gagcccgacc gccgattgtc cgcccgggag tcgccgaacg aattatctct ccgaggcgtc
     3241 agcgccgcgt catcgcgaac gacaaggcct tg
//
LOCUS       DATLJT010000171         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3914041,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000171 DATLJT010000000
VERSION     DATLJT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914041"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VM910_03820"
     CDS             complement(<1..754)
                     /locus_tag="VM910_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HVI61711.1"
                     /translation="MCGIAGFTGPHNRTALELMTECLRHRGPDSAGYWEGSDVSLGMR
                     RLAIIDVETGQQPVFNEDGTIAVVFNGEIYNYIELREALQRAGHRFSTDHSDTEVLVH
                     LYEDHGLAFLHQLNGMFAIALWDARRRQLVLARDRLGIKPLYFARIPGGVAFGSEPKA
                     LLKHPAISRAPNFAAIHHYLSLKNVPAPMSAFRDMEQLRSGELAICSGSQIERRRWWS
                     VEFDQCADLDESEAADQIRALLEDSVRLQMRSD"
     gene            853..1536
                     /locus_tag="VM910_03825"
     CDS             853..1536
                     /locus_tag="VM910_03825"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HVI61712.1"
                     /translation="MTTLVVLQPGYLPWLGYFDLLKKADVFVHYDDVQFDKHGWRNRN
                     RVKGPKGAIWLTVPVLHRGRAGQPILDVEIDNRQDWRRKHLSTVGQSYARAPFIDTVL
                     PKLQNIIERPWARLVDLDLALTDWLAAEIGIATPRYRASELDVGGDRNERLINLCHQF
                     RATRYISGNAAQDYLNVAQFAAAGIEVVWHNYVHPRYAQQHGEFVPYLSVVDLLLNVG
                     RDSLAVLSP"
     gene            complement(1547..>2239)
                     /locus_tag="VM910_03830"
     CDS             complement(1547..>2239)
                     /locus_tag="VM910_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HVI61713.1"
                     /translation="DAVRGLYAWRALGRSGGRVAVSVLADRLFSLFAVLCISLAFSLF
                     YWRQMQQVPALAALGTSIVVATVAFVAGACLLFAAPDLTRPIEARLLRWPMLVNLLAQ
                     ARGMILMLRTDPLSLLAAFALALATQILTVFAVAVLAGAIKIGVLNPIDYMFAVPFTL
                     IVNALPLTPSGIGVGEAAFDQICRWLEPTPSGAAYSSIFFAFRVVSTLTCIPGLISLA
                     IYRNAARSNPPR"
BASE COUNT          469 a          686 c          689 g          395 t
ORIGIN      
        1 catcggagcg catctgcagc cgaacgctgt cttcgagcaa agcacggatc tgatccgccg
       61 cctcgctttc gtccagatcg gcgcactgat cgaactcgac gctccaccag cgacggcgct
      121 cgatttggct gccagagcat atcgcaagtt cgccgctgcg cagttgctcc atatcgcgga
      181 acgcgctcat aggcgccggc acgttcttca gcgacaaata gtgatggatg gccgcgaaat
      241 tgggcgcccg cgatatggcc gggtgcttca gcagcgcctt gggttccgag ccgaatgcca
      301 cgccaccggg aatgcgcgcg aaatagagcg gcttgatgcc caaccggtcg cgcgcgagca
      361 ccagctgtcg gcgcctcgca tcccacagtg cgattgcaaa catgccattg agctggtgca
      421 ggaaggcgag accatgatcc tcgtagagat ggacgagcac ctcggtatct gagtgatcgg
      481 tagaaaagcg gtggcccgcg cgctgtagcg cctcgcgcag ctcgatgtag ttgtagatct
      541 cgccgttgaa gacaacggcg atcgtgccat cctcattgaa aaccggctgc tggccggtct
      601 cgacgtcgat gatggcgagc cggcgcattc ccagcgaaac atccgatcct tcccaatagc
      661 ccgcggagtc gggaccgcga tgacgcaggc actccgtcat caattccagc gctgttcgat
      721 tgtgtggccc ggtgaagcca gctatgccgc acattgtgac tctgtcgccg atgcgaaaga
      781 atagggcagc gtgatctccc cggcaggtgg ccggcttctt agcaggtccg cgcgcagacg
      841 caaaggatac tcgtgacgac gctcgtagtt ctgcagccgg gttatctgcc gtggctggga
      901 tattttgatc tgctgaagaa agccgacgtg ttcgtccatt acgacgacgt gcagttcgac
      961 aagcacggtt ggcgcaatcg caaccgggtc aagggtccga agggcgccat ttggctcacc
     1021 gtgcctgttc ttcaccgcgg acgcgcaggg caaccgattc tcgacgtcga gatcgataac
     1081 cggcaggatt ggcggcgcaa acatttgtcg accgtcggcc aatcgtacgc gcgcgcaccg
     1141 ttcatcgaca ccgtgctacc gaagctgcaa aacatcatcg agcggccatg ggctcgcctc
     1201 gtcgaccttg acctcgcgct caccgattgg ctcgccgcgg agatcggaat tgccacgccg
     1261 cgttaccgcg cgtccgagct tgacgtcggc ggcgatcgca acgagcgact catcaacctt
     1321 tgccatcaat tccgcgcaac gcggtatatc tcgggcaacg ctgcgcagga ctatctcaac
     1381 gtggcgcaat ttgcagccgc cggcatcgag gtcgtctggc acaattatgt ccatcccaga
     1441 tatgcgcagc agcacgggga gttcgttccc tatctgtcgg tggtggacct tcttctgaac
     1501 gtcgggcggg attcgctggc tgtgttgtcg ccatagcggg caatggtcac cgcggcgggt
     1561 tagaacgcgc cgcgttgcga tagatggcga gggaaatgag tccagggatg cacgtcagcg
     1621 ttgaaacgac tcgaaatgcg aagaatatgc tggaataggc ggcaccactc ggcgttggct
     1681 ccaaccagcg gcagatctgg tcgaacgcgg cctcgcccac cccgattcca ctgggggtca
     1741 gcgggagcgc gttcacaatc agggtgaacg gtacagcaaa catatagtcg atcgggttga
     1801 gcacgccgat cttgatcgct ccagcaagca cggccacggc gaacaccgtc agaatttgcg
     1861 tcgccaatgc caatgcgaac gccgcgagca acgagagtgg atccgtccgc agcatcaaaa
     1921 tcatcccccg tgcctgcgcg agcagattga ccagcatcgg ccagcgcaat agacgcgcct
     1981 cgatcggtcg tgtgagatcc ggagccgcaa acagcaggca ggccccagcg acaaatgcta
     2041 cggtagcgac gacgatcgac gtgccgagtg cggcaagcgc aggcacttgc tgcatctgac
     2101 gccaatagaa caagctgaag gccagggaga tgcagaggac ggcgaacagg ctgaacagcc
     2161 ggtcggcgag gaccgacacg gcgacgcgcc cgccgctgcg gccaagcgcg cgccaagcgt
     2221 aaagtccccg aactgcgtc
//
LOCUS       DATLJT010000172         5686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2765634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000172 DATLJT010000000
VERSION     DATLJT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VM910_03835"
     CDS             <1..222
                     /locus_tag="VM910_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI61714.1"
                     /translation="QESNTRTGWTVGAGYEYALGYGWSIKGEYLYVKFDDYTTFTQPP
                     FGGFGLNIAPRSVKLYDNVIRAGMNYKFW"
     gene            551..1513
                     /locus_tag="VM910_03840"
     CDS             551..1513
                     /locus_tag="VM910_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61715.1"
                     /translation="MRRRDVITGIAIAATAWPPAAHAERWPTQLVRIVCPLAAGGGID
                     ATARIVAARLSEIWRQQVVVENKSGASGNIAAEFVARSDPDGYTIYIATFPHATNSYL
                     YPSLGYDPVADFAPVTLIGLYPLMMVVPNSSPAHSVEEFIAYAKVNKLSYASSGHGTS
                     LHLAGELFKRRAGIEMTHVPYRGAGPAFNDLIPGRVDAMINFTASSLPLVRQGQLRAL
                     ALASAQRLAVAPELPTMAEAGVAGVEVSSWSAFFVPAKTPQDIIRKIHDDTLAVLAEP
                     AVRGKLEQVGVVVIGSTPDELAAFLSAEMDKWGHVIKAANIRAQ"
     gene            complement(1617..1919)
                     /locus_tag="VM910_03845"
     CDS             complement(1617..1919)
                     /locus_tag="VM910_03845"
                     /inference="COORDINATES: protein motif:HMM:NF018151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HVI61716.1"
                     /translation="MTKAIGFVSALIFVLGTVPAARAQLMLDVSKITCDQFAGYKITT
                     PQNIAIWLNGYYNGKRSNTVIDMQKLTENTKTLERYCIQNPQILVMKAVETVFGEK"
     gene            complement(2006..2836)
                     /locus_tag="VM910_03850"
     CDS             complement(2006..2836)
                     /locus_tag="VM910_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI61717.1"
                     /translation="MTQSAGSDPHALDRPWRLWASIAVFAIALSGVVLGVLIIPVVQG
                     RSDGIDAYTAICRALGILPGSPARQQVPDRTPPTPVSKVVWTPDLLQILANAKAERGS
                     AKAQEVCVACHGEQGVSRAPEYPNLAGQSGAAIYKQLSDYRTGSRTSPLMTEIAKALD
                     EAALADIAAYYAAQPKRNPNPATLAESPPAIVRLVELGDPSRNIPPCASCHRPGSGGP
                     IETPILSEQGKDYIVLQLKEYAAGNRRNDVYGRMRAIASKLTQTEIDGLAGYYRAGFR
                     "
     gene            complement(2847..4463)
                     /locus_tag="VM910_03855"
     CDS             complement(2847..4463)
                     /locus_tag="VM910_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI61718.1"
                     /translation="MRNVERLCLAHFWVAFGAFLIAACLGTWQMWVRSPLGANIGTPG
                     QYFISVTAHGVSMAYVMTTFFIMGFGYFVAVTALGRPLPGKPWAWLGFWMGFIGVFMA
                     LIPVLMGQASVLYTFYPPLTGSPWFYIGLVLVVAGSWIWCVLMLVAMREWKRENPGRP
                     VPLAMFGTVANALLWLWTTVGVAAELLFQVIPAALGFVQTIDAGLARTLFSWTLHAIV
                     YFWLIPAYIAFYTIVPRAAGGRLYSDTMGRLTFILFLLYSLPVGMHHLLMDPEHGNGW
                     KFIQVLLTAFVSVPTLLTIFTITASLEIAGRLRGGRGVFGWIAALPWERPIVLAAGLS
                     FVMLGFGGFGGLINMGYGMNAMVHNTSWITAHFHLIFGGSAVIMYFAIAYEIWPRLTG
                     HAHASTVPLRLQLWLWFIGMMVMTLPWHWLGLQGQWRRVANFNYADPIIAGWGPLVIV
                     SFAGGLVLLASALLFVRNLAVLRTSTLPALQRPLYALAVDPPQRVPAALNGFGLWNVL
                     VLVLMLLAYGYPIAQFAIAPSPTAMVHRMY"
     gene            complement(4450..5010)
                     /locus_tag="VM910_03860"
     CDS             complement(4450..5010)
                     /locus_tag="VM910_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HVI61719.1"
                     /translation="MTEQPVEGHERRVLGTELLWAFGVGVLIAAILGMIVFTALTRDI
                     NPPSNVEFIDPKTLHLSGEFAEQNLGTTVAADGGVTVRIIATQFMFVPRCIAVPRGRR
                     VTLRFAAPDVVHGVLITGTNVNTMVVPGFVAQVYTEFTTQGGDLLMPCHEYCGLGHSD
                     MWANVQVVPESEFKPDRDGRVSCAQR"
     gene            complement(5014..5214)
                     /locus_tag="VM910_03865"
     CDS             complement(5014..5214)
                     /locus_tag="VM910_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61720.1"
                     /translation="MIAADQASAADVPSEVAAQVDEAALTEVMRVVPLGAGVVAGVTV
                     ALLVIGYLAIYLLVFLPRGGVG"
     gene            complement(5363..>5686)
                     /locus_tag="VM910_03870"
     CDS             complement(5363..>5686)
                     /locus_tag="VM910_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI61721.1"
                     /translation="VAEVGENIRAGQPVLAIEQAGKQWLSFNVREDNLHGIRVGMIVD
                     VRRMGTSETSPALVTELLPLGTFATWQAERAVGDYDRNTLRLRLDPQRDATAFEPGMT
                     VWFVR"
BASE COUNT         1176 a         1766 c         1715 g         1029 t
ORIGIN      
        1 caagagagca atacccgcac cggttggacg gtgggcgccg gctacgagta cgcgctcggt
       61 tacggctggt cgatcaaggg cgagtatctc tatgtcaagt ttgacgacta caccacgttc
      121 acgcagcctc ccttcggagg ctttggcctg aacatcgcac cgcgcagcgt caagctctat
      181 gacaacgtca ttcgcgccgg catgaactac aagttctggt aatcaagagc cccccaaagc
      241 tcttgagttg aggaagccct cggcaggatt gaaccccccg ccgagggctt ccatttgcct
      301 gaacgcggac gaaatagtgg tcccggtttt tcagcggatc gcggacacga ctgcggccca
      361 atctcgaacg gcgcttttgg gcgaagcgca agcgcatgca cgctgagcat cgattaccgc
      421 tcggcagcgg atgacccgat ccccctcgtc cggcctcttg tgaacgctca tcggcgccga
      481 tcctgcgtcc tggcaggtgt ggtatccttc gaattgcggt tggcgcggtc tggggctgcg
      541 ggaggaccgc atgcgacggc gcgatgtcat caccgggatt gcgatcgcgg cgaccgcgtg
      601 gccgcccgct gcgcacgcgg aaaggtggcc cacgcagttg gtgcgcatcg tctgcccgct
      661 cgcagcgggc ggcggaatcg acgcgactgc ccgtatcgtc gcggcgcggc tttcggagat
      721 ctggcgccag caggtcgtcg tcgagaacaa gtccggcgcc agcggcaaca ttgcagccga
      781 attcgttgcc cgttccgacc ctgacggcta caccatctac atcgccacat tcccgcacgc
      841 gaccaacagc tatctctatc cctcgctcgg ttacgacccg gtcgccgact tcgcgcccgt
      901 cacgttgatc ggcctctacc cgctgatgat ggtcgtgccg aactcttcgc cggcacactc
      961 ggtcgaggaa ttcatcgcct acgcgaaggt gaacaagctc agctatgcct cgtccggcca
     1021 cggtacgtcg ctgcatctcg cgggcgagct gttcaagcgc cgggccggca ttgaaatgac
     1081 ccacgtgccc tatcgcggag cgggacccgc cttcaacgac ctgatccccg gtcgcgtcga
     1141 tgccatgatc aacttcaccg cgtcgagcct gccgctcgtc cgccagggtc agttgcgcgc
     1201 gctcgcgctg gcgagcgccc agcggctcgc ggtcgcgcca gagctgccga ccatggccga
     1261 ggccggcgtt gcgggagtag aggtgtcttc atggtcggcg ttcttcgttc cggccaagac
     1321 gccgcaggac atcatcagga aaattcacga cgatacgctc gcagtgctgg ccgagccggc
     1381 ggtcagagga aagctcgagc aggtcggcgt cgtggtaatc ggctcgacgc cggatgagct
     1441 tgccgctttt ctcagcgccg agatggacaa atggggacac gtcatcaaag cggcaaacat
     1501 cagggcccaa tgaagcagcc ggcagccttg cgctggcccg ggttacggcg tgatctgctt
     1561 ctaaacgttt gcggacatct cccgccgcgc aaaaggcgcg gctgtcttcg gcgctcctat
     1621 ttttccccaa acactgtttc gactgctttc atgaccagga tttgaggatt ttgaatgcag
     1681 tatctttcta gcgtcttcgt attttcggta agcttttgca tatcgatgac tgtgttactt
     1741 cgcttgccgt tataatagcc gttcaaccaa atcgcgatat tttgaggagt ggtaattttg
     1801 tagccggcga attggtcaca cgtgatcttt gaaacgtcca gcatgagctg cgcccgggct
     1861 gcggggacgg tcccaaggac gaatatgagt gctgatacaa aaccaatagc ctttgtcatg
     1921 accttctcct aagagagata tccccggatc gtatcggggc cggatcgtgt agggcaggag
     1981 gcaaaccgtg acaggacgtc tcgcgctatc tgaaccccgc tcgatagtat cccgccagtc
     2041 catcgatctc ggtctgcgta agctttgagg cgatcgctcg catgcgccca taaacgtcat
     2101 tgcggcgatt acccgcggca tactccttca attgcaagac aatgtagtct ttcccttgct
     2161 cggaaagaat tggcgtctcg atcggaccac ccgaacccgg tcgatggcat gacgcgcagg
     2221 gcgggatatt gcggctggga tcgcccagct cgaccaggcg cacaatcgcg ggaggagatt
     2281 ccgcgagtgt tgctggattt gggttgcgct ttggctgagc cgcgtaatat gcagcaatat
     2341 cggcgagcgc ggcctcatcg agggccttcg cgatctctgt catcagcgga ctagttcggc
     2401 tgcccgttcg atagtcgctc agctgcttgt aaattgccgc gcccgattga ccggcaagat
     2461 tgggatattc aggagcccgc gacacgccct gctcgccgtg acaagcgacg cagacctcct
     2521 gtgccttggc agagccccgt tctgctttgg cgttggcgag aatttgcaat aggtccgggg
     2581 tccacaccac cttggacacc ggcgtgggtg gcgtgcggtc aggaacctgc tgccgtgctg
     2641 gcgatcccgg caagatgcct agtgcccggc agatggcggt ataggcgtcg attccgtcgc
     2701 ttcttccctg gaccacggga atgatgagca cgcccagaac aacgccgctg agcgcgatgg
     2761 cgaaaacggc gatgctggcc caaagccgcc acggccgatc gagagcatga gggtcggagc
     2821 ccgctgattg cgtcatcgcg cgcttcctaa tacatccgat gcaccatcgc cgtgggcgag
     2881 ggtgcaatcg cgaactgtgc gatcggatat ccgtaagcca gcagcatcag gacaagaacc
     2941 aagacgttcc agagcccgaa gccgttgagt gcagccggaa cgcgctgcgg cgggtccacg
     3001 gccagcgcgt acagcggtcg ttgcagcgcg ggcaacgtgc tggtgcggag gacggcgaga
     3061 ttgcgcacga atagcagtgc ggaggcgagc agcactagac cgccggcgaa cgagacaatg
     3121 accagcgggc cccagccggc aatgatcgga tcggcatagt tgaaattggc gacgcgccgc
     3181 cactggccct gcaagcctaa ccagtgccat ggcagcgtca tgaccatcat cccgatgaac
     3241 catagccaca gctgcagccg tagcggcacg gtcgacgcgt gtgcgtgccc ggtcaggcgc
     3301 ggccaaatct cgtaggcgat ggcgaaatac atgatgacgg cggagccgcc gaaaatcagg
     3361 tgaaagtgcg ccgttatcca cgaggtgttg tgcaccatcg cgttcatgcc gtagcccatg
     3421 ttgatgagcc cgccgaagcc gccgaagccg agcatcacga aggaaaggcc ggccgcgagg
     3481 acgatcggcc gttcccatgg cagcgccgcg atccaaccga acacaccacg gccgccgcgc
     3541 aagcgtcccg cgatttccag cgatgccgtg atcgtgaaaa tcgtcagcag cgtcggtacg
     3601 gacacaaagg ccgtgagcag gacctggatg aacttccagc cattgccatg ctcgggatcc
     3661 atcaacaggt gatgcattcc gacgggcagg ctgtagagca ggaatagaat gaaggtgagg
     3721 cggcccatcg tatcgctgta gaggcgtccg cccgccgctc ggggcacgat cgtataaaac
     3781 gcgatgtaag ccggaatgag ccagaaatag acaatggcat gcagcgtcca cgaaaacagc
     3841 gtccgcgcaa gccctgcatc gatcgtctgc acgaagccaa gcgccgccgg gatgacttga
     3901 aacagcagtt cggccgccac gccaaccgtg gtccaaagcc acaacagcgc atttgccacc
     3961 gtcccgaaca tcgccagcgg cacaggtcgg cctggatttt cgcgcttcca ctcgcgcatc
     4021 gcgacgagca tgagaacgca ccatatccaa gaaccggcca ccacgagcac caacccgata
     4081 tagaaccacg ggctgccggt gagcggagga taaaacgtat agagtaccga ggcctgaccc
     4141 atgaggaccg ggatcagcgc catgaacacg ccgatgaacc ccatccagaa gccgagccat
     4201 gcccaaggtt tgcctgggag aggacggccg agcgcagtca cggcgacgaa atagccgaag
     4261 cccatgatga agaacgtggt catcacgtag gccatcgaga cgccatgcgc agtcacggaa
     4321 atgaaatact gccccggcgt gccaatgttg gcgcccaatg ggctgcgcac ccacatctgc
     4381 caggtgccaa ggcaggccgc gatcagaaac gctccgaacg cgacccaaaa atgcgcaagg
     4441 caaagccgct caacgttgcg cacaggagac cctcccgtct cgatcgggct tgaattcgct
     4501 ttccggcacg acttgcacgt tggcccacat gtcgctgtgg cccagaccgc aatactcatg
     4561 acacggcatg agcaggtctc caccttgggt ggtgaattcg gtgtacacct gcgcgacaaa
     4621 gccggggacc accatggtgt tcacattggt cccggtaatg aggacaccgt ggacgacatc
     4681 cggtgctgcg aagcgcaagg tgacgcgccg cccccgggga accgcgatgc agcgcgggac
     4741 gaacatgaat tgcgtggcga tgattcgcac agtgacgccg ccgtccgccg cgaccgtggt
     4801 tcctagattt tgttcggcga actcgcctga cagatgcaat gtcttcgggt cgataaactc
     4861 cacgttgctg ggcgggttta tgtctctggt cagggccgtg aacacgatca tgccgagaat
     4921 cgcggcaata agcacgccga cgccgaacgc ccaaagcaat tcggtgccga gaactcggcg
     4981 ctcgtgccct tcaactggtt gctcagtcac gggctagccc accccacctc gcgggaggaa
     5041 gacgagcaga tagatcgcca gatagccgat caccaacaag gcgacggtga ctccggctac
     5101 aacaccggct ccgagaggca cgacgcgcat gacctcggtc agcgcggctt catcgacttg
     5161 agcggcgact tcagacggaa cgtcggcagc ggaagcttga tcagcagcga tcatcggcct
     5221 gccccccatc gtgcgccagc taatgtcctc gatgctgaat tctacaccga ggtcgtcaaa
     5281 aggccagcga cttcaaggaa gccaatgatc gtcccggttc aatacaacgc gtggcggctg
     5341 ttcaccgcaa ttgcgagtgc tctcagcgaa cgaaccacac tgtcatgccg ggctcgaagg
     5401 cggtcgcatc gcgttgcgga tcgaggcgca ggcgtagcgt gttgcgatca taatcgccga
     5461 ccgcgcgctc ggcttgccag gttgcaaatg ttcccagcgg caggagctcg gtgacgagcg
     5521 ccgggctcgt ttcgctcgtg cccatcctcc tcacgtcgac gatcattccg accctgatgc
     5581 catgcagatt atcttcccgc acgttgaagg acagccattg ctttccagct tgctcgattg
     5641 ccagcaccgg ctggccggcg cggatgttct cgcccacctc ggcaac
//
LOCUS       DATLJT010000173         3422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_237824,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000173 DATLJT010000000
VERSION     DATLJT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237824"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..959
                     /locus_tag="VM910_03875"
     CDS             96..959
                     /locus_tag="VM910_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61722.1"
                     /translation="MQYDDKLLYKPSSGCHFVNKEFSTDLTFSEDGRRTTSSRAPASR
                     RPLIFAGDSETMGWGVNDGETFASLIAASIEAPVLNLGVASYGTVREIMRIRMQPRFQ
                     DANCIVIQYSWNDFDENSVFLARGALPSPTRRRFQQLVSDYGKRDVSFPDVMLRSLDV
                     MWNNPLAFFLNILGWHDFPLEDDDPMNMDGTPREEAEDTKAFLAVLASFSELNGKKIF
                     VMGPGRFISALSRETPPANVFPLSVDLNDGDWYTLDMHPNKQGHREMAAQILNELKRT
                     EQGRQCLAGEP"
     gene            complement(1036..1464)
                     /locus_tag="VM910_03880"
     CDS             complement(1036..1464)
                     /locus_tag="VM910_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61723.1"
                     /translation="MWFFNQKRADRVAQSISHLIHTLSAGRENHGPTGPERLLRELAT
                     LLSEDTIIGQRVVKLLMATSTKAKFLVSLATTPDHSSHRLTFDGRGIYSGFSLACFLP
                     PRTVMIDLHQGAVGQHARLLYAACKAMPNVVERRDFRWDR"
     gene            complement(1621..2730)
                     /locus_tag="VM910_03885"
     CDS             complement(1621..2730)
                     /locus_tag="VM910_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI61724.1"
                     /translation="MSVYKNRDGRSPYFHFDFQVRGHRFHGSTKCTTRREAEKVEAAE
                     REKATRLVAQLAAAKTSLRLDDVADRYWTEHAQHLSGAANIWCHLGLALDFLGKNTLI
                     TDLSDDDVARFVAWRRGQLNRGRGLISPLTVNETVASLRKLFARAKLWGVRFEHEPRW
                     TKHMLAVPTERVRELSDNEADRLDAAMRADYAPFFAFARASGLRLRECFLRWSEIDWN
                     ARQITKLGKGGRRVTVPITATIRDILWPLRGHHPEFVFTYVAQRTRDGRVQDARFPLN
                     YYGVQSCWQRVRKLAGVTGFRFHDHRHDFATKLLRETGNLKLVQKALNHRNIRSTLRY
                     AHVLDSEVAEAMERVAKSRNRSRSSALRVVKPLAG"
BASE COUNT          692 a         1005 c         1082 g          643 t
ORIGIN      
        1 agcgtcatca gaacacgcgc agaatttgga acgtcattgc gaccgacctt tacttcgagt
       61 acgaggccaa cctatggcag aatcagaagg actgcgtgca gtacgacgac aagttgctgt
      121 ataagccgtc gagcggctgt catttcgtca acaaggaatt ttcaaccgac ctcaccttct
      181 cggaagacgg aaggcgcacg acatcttcac gcgcgcctgc gtcgcgacgg ccgctcattt
      241 ttgcgggcga ttccgaaacc atgggatggg gggtgaacga cggcgagacg ttcgcgtcgc
      301 tcattgccgc cagcatcgag gcgccggttt tgaacttggg cgtggcaagc tacgggacgg
      361 tcagagagat catgcgcatt cgcatgcagc ctcgttttca agacgccaat tgcatcgtca
      421 ttcaatacag ctggaatgat ttcgacgaga acagcgtatt tctggcgagg ggtgcgttac
      481 cttcgcccac gcggagaagg tttcaacagc tggtgtcgga ctatggcaag cgagacgtaa
      541 gcttcccgga cgtcatgcta cggagcttgg acgtcatgtg gaacaatccg cttgcgtttt
      601 tcttaaacat ccttggatgg catgacttcc cgttggaaga tgacgacccc atgaacatgg
      661 atgggactcc cagagaagag gccgaagata cgaaggcgtt cctggccgtt ctggcgtctt
      721 tctccgagtt gaacggcaaa aagatcttcg tgatgggccc cggccgcttc atctccgcac
      781 tcagccgcga aaccccgcct gcaaacgtgt tcccgctctc ggtcgatttg aacgacggcg
      841 actggtacac gctcgatatg catcccaata agcaggggca tcgggaaatg gccgcgcaaa
      901 tattgaatga gctcaagcgg accgaacaag gcaggcaatg cctggcgggt gaaccctgac
      961 caagccgatg taacaggcgg tctacaagtc cttggaatgc acgcggcgac ggcagcggac
     1021 gcgaccgcca tcatttcagc ggtcccagcg aaagtctcgg cgttcgacca cgttgggcat
     1081 ggctttgcag gccgcgtaga gtaggcgtgc gtgctgtccg accgcgcctt gatgcaaatc
     1141 gatcatcacc gttcgaggcg gcaagaagca ggcgagcgaa aagccggagt atatgccccg
     1201 cccgtcgaac gtcagccgat ggctggaatg atccggcgtg gtcgccaaac taaccaagaa
     1261 cttggccttg gtgctcgtcg ccatgagcag cttgacgacc cgctggccaa tgatcgtgtc
     1321 ctcgctgagc aacgtcgcga gttcgcgcag gagccgttcc ggacccgtcg gcccgtgatt
     1381 ttcccgcccc gcagacagcg tgtggatgag gtggcttatg gactgagcga cccggtctgc
     1441 ccgtttttgg ttgaaaaacc acatggaact aactcaccac tccacggcgt gcgccggcgg
     1501 ccccgcctcg ttcactgatc agaaactagc gtcgtcggtc cgccctcggc aaccgcgcca
     1561 tgtctgaaag ttgaccgctg tgacattttt acaaaatgat gggcgcggcc gcaatcatcg
     1621 ctatcccgcc aatggcttga cgacacgaag cgcgctgctc cgggagcgat tccgggactt
     1681 cgcgacgcgc tccatggcct cggccacttc cgagtcgagg acgtgcgcgt aacgcagcgt
     1741 gcttctgatg ttgcggtgat tgagcgcctt ctgcacgagc ttgaggttgc cggtctcgcg
     1801 caagagcttg gtggcaaagt catgccgatg gtcatgaaag cggaagccgg tgacgcccgc
     1861 gagcttgcgc acgcgctgcc agcaactctg cacgccgtag taattcagcg gaaagcgcgc
     1921 gtcttgcacg cggccatcgc gagtacgttg cgccacatag gtgaatacga actcgggatg
     1981 gtgcccgcga agcggccaaa ggatatcacg gattgttgca gtgatcggca cggtaacgcg
     2041 gcggccgccc ttaccaagct tggtgatttg cctcgcgttc cagtcgatct ccgaccagcg
     2101 taggaagcat tcgcgcagcc gcaacccgga ggcgcgggcg aaggcgaaga acggcgcgta
     2161 gtccgcgcgc atggcggcgt cgagccggtc ggcctcgttg tcggatagct cgcgcacgcg
     2221 ctcggtgggg accgcgagca tgtgctttgt ccatcgcggc tcgtgctcga agcggacgcc
     2281 ccacagcttt gctcgcgcga acagcttgcg cagcgaggcg acggtctcgt tgacggtaag
     2341 gggggagatt aaaccgcgac cacggtttaa ttgcccgcgc cgccaggcca cgaagcgcgc
     2401 cacgtcatca tccgagaggt cggtgatcag cgtgttcttc ccgaggaagt ccagcgcgag
     2461 accgagatgg caccagatgt tggccgcgcc ggacagatgc tgcgcgtgct cggtccagta
     2521 ccgatcggcg acgtcgtcga ggcgcagcga ggtcttggcc gccgccagct gcgcgacgag
     2581 ccgcgtcgcc ttctcgcgct cggccgcctc tactttttcg gcttcgcgcc gcgtcgtgca
     2641 tttcgtcgag ccgtgaaacc gatgacctcg cacttggaag tcgaagtgga aatacggcga
     2701 gcggccgtct cggttcttgt agacggacac ggggaatcct ttcgggtttg attggcaatg
     2761 aaatcatcga gatcggcgtc ggtgaacatg cggcgcgggc gtttgcggcc gtgcccgatg
     2821 atgacatagc ggagcgcgcc ggaagcgacg tgaccagcaa gcgtcttaac cgagcagccg
     2881 aggcgacgtg cggcatcggc aggcgtgagc agggaattag gtgggcccat ggagttcacc
     2941 gacgcaatgg ccgggccctg cggtcgcacg gacgaactgg agttcagata tggaccggcg
     3001 accgcaatgg gatttcgatt tatgtcgtga tcactgtgca tcctgtcagg cggcggggaa
     3061 gctgcctgcc gtcggagcgc ggttttgacc gtcgcgacca tctcgtcgcg ctgctcgcgc
     3121 agaaaaccgg gaaggggcat gccgccgatc agcgcggcaa tgcgctcgtc ggatacggac
     3181 gcgacccaat ccgcgacttt cgcggcatcg agcatcggcc aattcgtgct atcaggaatt
     3241 tggatcattc gcgccgcctc caacggccgc ttgatctatg ccccggctgt cgccaaccac
     3301 ggcggtcggg gcgcttcaat ttaggccgaa gacgcgacgg cggacaagga agcaaagatc
     3361 tgtccaaaaa atcgtccggc gaacggcctg aaaaagcgtc gggcgaatgg gcctgagaaa
     3421 ac
//
LOCUS       DATLJT010000174         7487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3454699,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000174 DATLJT010000000
VERSION     DATLJT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454699"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VM910_03890"
     CDS             <1..1173
                     /locus_tag="VM910_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61725.1"
                     /translation="ASLVFLTAATAIYTALGGIKAVVWTDVIQAMVMFGSLAFAIWSL
                     LHAIPDGWQGAVALLNAPKDMIFFDSGTEPNASFLENVRKVLGTEYTLWAALFGSTFI
                     TMATHGTDQDMVQRMLTAKNATKSRLALILSGLADLPIVLCFLTIGILLWAFYQKQEM
                     PHPFAYYILHEMPPGVRGLMIAGIFATAMGSLSTALNALATSFTQDWYLAYIRPGAAP
                     QHVVTAARKATVVFSAILVVIGSATAYAAIVLHSRVIPIVLGIFGYTYGSLLGVFLVG
                     MLTKTRGSEKGNLVAMACGFVAVSFLSGLHNDLWNLLHPSDPDFLTWMEGHPRGPAFQ
                     HWKWDWLPTIDFQWRITVGTLVTAAVALIFRTPPRHGTSCEACPQPGSEVRANPLP"
     gene            1634..2164
                     /locus_tag="VM910_03895"
     CDS             1634..2164
                     /locus_tag="VM910_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI61726.1"
                     /translation="MKIQKADEVADFTSHLSDIDDIFFESSAKHDFCNEEEKQFFREM
                     SLGRYIVRHQDSFFVALDDAGRAVGYLAGCLENPTKLDHFNDIAYFRYIDDICQNYPA
                     HLHVNVADQYRNRGLGTALVERFAELAKLHSVEGIQVVTSSTSRSIPFYRRLGFRELR
                     TFPWMSVTSVCMGRKL"
     gene            complement(2773..3465)
                     /locus_tag="VM910_03900"
     CDS             complement(2773..3465)
                     /locus_tag="VM910_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI61727.1"
                     /translation="MALKLYFHPLASFCHKALIALYENRIPFEPIIVDLRDEASSAAF
                     RAVWPMAKMPVLRDEARNRTVAESTIVIEFLDAHYPGPTRFLPADVDRAWQTRMWDRF
                     HDHYVQEPMQKIVADRLRPEGKADAYGVELAKAQLQRAYGVMEREMDSKTWAIGDDFT
                     LADCAAAPALFYANTVVPFAETHKNLAAYLDRLMARSSFARVLEEAQPYFHLFPLERK
                     PTIARHGRVASR"
     gene            complement(3529..4068)
                     /locus_tag="VM910_03905"
     CDS             complement(3529..4068)
                     /locus_tag="VM910_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI61728.1"
                     /translation="MVLKILIALVAIVAAFAAFVALQPSEYRIARSATIAAPASDVFA
                     QVNDFHKWDAWSPWAKLDPNAKTTLEGPSAGEGAVFAWAGNSQIGEGRMTLTQSRPAE
                     LIRIKLDFVQPMAGTSTAEFTFKPEGERTAVTWAMFGHNNFVSRAICLFMNQDKMLGG
                     YFEKGLENLKRVAEAAVRE"
     gene            complement(4118..4528)
                     /locus_tag="VM910_03910"
     CDS             complement(4118..4528)
                     /locus_tag="VM910_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61729.1"
                     /translation="MPTPYLFFDGRCEEAIEFYKKALGAEIKMMMRYKESPEPPPPGM
                     VAPGSDNKIMHSCLAINGEDVMASDGCAQGKPKFEGFSLSINARNEAEADRVFAALAD
                     RGEVRMPLGKTFFAKRFGTVADRFGVGWMIILEA"
     gene            complement(4603..5097)
                     /locus_tag="VM910_03915"
     CDS             complement(4603..5097)
                     /locus_tag="VM910_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61730.1"
                     /translation="MELTAKKITPCLWFDTQAEEAAKFYASVFKNSTVGKISRYGKEG
                     FEVHGKKAGTVMTVEFELEGQKFLALNGGPHFKFNEAVSFQVHCETQQEIDHFWSELA
                     KDGEEGPCGWLKDKFGLSWQVIPKALPEMLMDGNSETAQRVMRSMLQMRKIDIAALKR
                     AQAA"
     gene            5334..5780
                     /locus_tag="VM910_03920"
     CDS             5334..5780
                     /locus_tag="VM910_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI61731.1"
                     /translation="MSKAAEVPFATTLLVRDTCLCLHLQRAARALARRFDDALRPLGL
                     TSGQYSTMMSLNRPEPPSIGSVASLLAMDRTTLTAALKPLQRRGLITVMVDAKDRRSR
                     RLKLTPAGRALLARAVPVWRRTHGEIERLVPRAGADRLRRDLRALS"
     gene            5955..6971
                     /locus_tag="VM910_03925"
     CDS             5955..6971
                     /locus_tag="VM910_03925"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HVI61732.1"
                     /translation="MHDANALITRLPKCELHIHVEGSLEPELMFALARRNGISLPYAS
                     VDAVRQAYRFGNLQDFLDLYYRGMSVLITEQDFYDLAFAYLARAHADNVRHVEMFFDP
                     QGHTSRGVAFATVVEGLTRAIADARRELGVEASLIMCFLRHLDEADAERTLDAALAFR
                     DRIVGVGLDSSERGNPPGKFTRVFARARDAGFFLTAHAGEEGPASYVWEALDLLGVAR
                     IDHGVRAMEDQALVGRLAREQVPLTVCPLSNVRLRVVDDLAHHPLRRMLDKGLMVTVN
                     SDDPAYFGGYVNENYRASAQALGLARDEIAALVRNGIRASLMTTPEKDKALADVDRVL
                     AGTV"
     gene            complement(7034..>7487)
                     /locus_tag="VM910_03930"
     CDS             complement(7034..>7487)
                     /locus_tag="VM910_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61733.1"
                     /translation="GFLAWGIVAARYLWPYLRSQPRIDALRPLLILHSFRFVGLAFLV
                     PGVVAPDLSAAFARPAAYGDLIAAVLALLALASLQRRWGMPLVWVFNLWGTADLLYAF
                     YQGNSVGFAPGQLGAAYFIPTVVVPLLLITHGLMFRLLLRGEDVRASP"
BASE COUNT         1384 a         2299 c         2242 g         1562 t
ORIGIN      
        1 gcctcgctgg tgtttctgac cgccgcgacc gcgatttaca ccgcactcgg gggtatcaag
       61 gcggtcgtct ggacggacgt catccaggcg atggtcatgt tcggttcgct cgcatttgcg
      121 atttggtcgc tgcttcacgc gatcccggac ggctggcagg gggcggtggc cttactcaac
      181 gctcccaaag acatgatttt tttcgattcg ggtaccgagc cgaatgcctc ctttttggaa
      241 aatgtccgca aggttttggg gacggaatac accctctggg ctgccctctt cggctccacc
      301 ttcatcacaa tggccacgca cggcaccgac caggacatgg tgcagcggat gttgacggcc
      361 aaaaatgcta ccaagagccg cctggcgttg atcctttctg gtctggccga tctccccatc
      421 gtcctctgtt ttctcacaat cggcattctg ctctgggcgt tctatcagaa acaagagatg
      481 ccccatccct tcgcgtatta catcttgcat gaaatgccgc cgggcgtgcg cgggctgatg
      541 atcgcgggaa tcttcgccac ggcgatgggg tcgctttcca ctgccctcaa cgcgctggcc
      601 accagcttta cgcaagactg gtatctcgct tatatccgtc cgggagcggc tccccagcac
      661 gtcgttaccg ccgcgcgcaa ggccacggtt gtcttttcgg cgatcctggt tgtcatcggc
      721 tcggccactg cttatgccgc catcgttttg cactcgaggg tgattcccat tgtgctcggc
      781 atctttgggt acacctacgg ctcgcttctg ggagttttcc tagtgggcat gctcactaag
      841 acgcgcggca gcgagaaggg taatctcgtg gcgatggcgt gcggatttgt tgccgtgtca
      901 ttcctgagtg gcctccacaa cgacctctgg aatctgctgc atccttcgga ccccgacttt
      961 cttacttgga tggaggggca tccgagaggc ccggctttcc agcattggaa atgggactgg
     1021 ctgccaacta tcgatttcca atggcggatc accgtcggga cattggtgac agctgccgtg
     1081 gctttgatct tccgcacccc gcctcgacac ggcacctcat gcgaggcatg tccgcagccg
     1141 ggaagcgagg tgagggcgaa tcctttaccg tgatatggca aggaggtccc caaagcgttt
     1201 gctttcgcgt gaatggccgg tgggtgaggc tcggagatct cgactatagc ttccctcgat
     1261 tcgagatgga tttcgatcct gcggatcgaa tccgcgactg gctcaagcag cctttgctct
     1321 acttttaccg caagcgagcg ttccaacaat ggaacagcgt gtatacgtga tcgggcatcc
     1381 tggtgggtgg gatactcatc gcgatacgtt cgctatcgta gtcccacaga aggcggcagc
     1441 tcgggattgc cggtgttcga gcgccggctc aagtgctttt gaacgaaagg ttcgtcctcg
     1501 atgaaatcaa gaaggctatc gccgatatac caacgtgacc tggatcgcat cgcttgtgct
     1561 ggctgaccac actgaggcga aatccacttg aaggaaaatc gctgatttca tcacggggct
     1621 gtggagcgtg accgtgaaaa tacagaaagc agacgaagtt gctgatttca ccagtcatct
     1681 tagcgatata gacgacattt tcttcgaatc gagcgcaaaa catgattttt gcaacgagga
     1741 agaaaaacaa ttctttcgag agatgtcact cggacgatat attgttagac atcaagacag
     1801 tttttttgtt gcgcttgatg acgccggacg cgccgtcggg tatctcgcag gctgtctcga
     1861 aaatcccaca aagttggatc atttcaatga tatcgcatat tttcgctata tagatgatat
     1921 ctgtcaaaat tatccggcgc atcttcatgt taatgttgcg gaccaatatc gaaatcgtgg
     1981 gttgggaact gcgcttgtcg aacgatttgc agagttggcg aagcttcatt ccgtcgaagg
     2041 aatccaggtc gtgacatcga gtacgtctcg aagcatccca ttctaccgac gattgggctt
     2101 tcgtgaattg cgaacgtttc cgtggatgtc cgtgacctcg gtttgcatgg gccgcaaatt
     2161 atagaggcag tgatgtcggt cgggccagag agccccgcgc tgagctttgc gtcgtagcag
     2221 ttaaagctcc ggtgcggcta acgctgcggg ttggttaccg gcattctgaa aacagggcta
     2281 acctattgac gggtaatcag ttgaattttg ataatttttc agaataggcg ttgatgaggc
     2341 acgcgaaata gctggatttt gatcccgcca tttcgcagat cgatcttgcc gcaatggtga
     2401 tcgatcttcc tccttgacag gcccatcgtg gccgaatcaa gcgaatggag ttagttgtac
     2461 ggcctttgcg attgtcctgg tataggtctt gagcatggat ttatctccgc gggatcattc
     2521 aatgcaacgt agcgtctgcc gtcggtccac caagcatttg ctttgctaag tccgcgatgt
     2581 cgaggatcga gacatagatg tattccagga ataggtgcat ctctttaggc tctaaaagta
     2641 ccgaagcctt ggcaattttt cacgattgac gagaaatcac tgcagcgacc gggccgaggg
     2701 agcgccatct caatcgagat tcatcttcgt tgcgccgctg ctgcgacgtc gatatctgct
     2761 tgcgcgcccg cctcagcggc tcgctacgcg gccgtgtcgg gcgattgtcg gctttctctc
     2821 cagtggaaat agatgaaagt atggctgcgc ctcctcgagc acccgcgcaa aggaagagcg
     2881 cgccatcagg cggtcgagat aggccgctag atttttgtgc gtctcggcaa aaggaacgac
     2941 ggtgttcgcg tagaagagcg ccggcgccgc cgcgcagtcg gccaaggtga agtcgtcgcc
     3001 gatcgcccag gttttcgagt ccatctctcg ttccataacg ccgtaggccc gctggagctg
     3061 cgcctttgca agctccaccc cgtacgcatc ggccttgccc tcgggacgca gccggtcagc
     3121 gacgatcttc tgcatgggtt cctgaacgta gtggtcgtgg aagcggtccc acatccgcgt
     3181 ctgccaggcc cgatccacat ccgcgggcaa aaagcgcgtc gggcccggat agtgcgcgtc
     3241 caggaattcg atgacgatgg tcgattccgc caccgtgcga ttgcgcgcct cgtcgcgcag
     3301 caccggcatc ttggccatgg gccagaccgc gcggaaggcc gcgctcgaag cctcatcgcg
     3361 caaatcgacg atgatgggct cgaacgggat gcggttctca tacaaggcga tgagcgcctt
     3421 gtggcagaac gacgcaaggg ggtggaaata gagcttcagc gccatgccaa cggacccgcg
     3481 cgcgttccct cttgaacgtt ccgcttcgct gaaccgaagc cggcgcgact attcgcgcac
     3541 cgccgcttcc gccaccctct ttaagttctc caggcccttc tcgaaatagc cgccgagcat
     3601 cttgtcctgg ttcatgaaca ggcagatcgc ccgggagacg aaattgttat gcccgaacat
     3661 ggcccaggtg accgccgtcc gctcgccctc gggcttgaac gtgaactcgg ccgtgctggt
     3721 gccggccatc ggctgcacga aatcgagttt gattctgatg agctcggccg ggcggctctg
     3781 ggtcagcgtc atgcgccctt cgccgatctg gctgttgccg gcccaggcga agaccgcgcc
     3841 ttctccggcc gacgggccct cgagggtggt cttcgcgttg gggtcgagct tcgcccaggg
     3901 cgaccatgcg tcccacttat ggaagtcgtt cacctgggcg aagacatcgg acgcgggggc
     3961 ggcgatcgtc gccgaccgcg cgatccgata ttcggagggc tgcagcgcga cgaacgcggc
     4021 gaacgccgcg acgattgcga cgagcgcgat caggatcttc aggaccatcg aacccccctt
     4081 tgcaacgcca tcgatgcgcg ccgcccgcct cccgcgctca agcctccagg atgatcatcc
     4141 agccgacgcc gaagcggtcg gcgaccgtgc cgaagcgctt ggcgaagaac gtcttgccga
     4201 gcggcattcg cacctcgccg cggtcggcga gcgccgcgaa cacgcggtcg gcttcggcct
     4261 cgttcctggc gttgatggag agcgagaagc cctcgaattt cggcttgccc tgcgcgcagc
     4321 cgtcggaggc catcacgtcc tcgccgttga tggcaaggca tgaatgcatg atcttgttat
     4381 cgctgccggg tgcaaccatg ccaggcggcg gcggctccgg gctttcctta tagcgcatca
     4441 tcatcttgat ctcggcgccg agtgccttct tgtagaactc gatcgcctcc tcgcagcggc
     4501 cgtcgaagaa cagatacggt gtcggcatgg tctctctcct ttgtgcaagt cgttggggaa
     4561 aagtcgtttg ggaaagtcgt tgcgatgcgc aatgcggccc gttcaggccg cttgcgcccg
     4621 tttcagcgcc gcgatgtcga tcttgcgcat ctgcagcatc gagcgcatga cccgttgcgc
     4681 cgtttcggaa tttccgtcca tcagcatctc gggcagcgcc ttagggatga cctgccatga
     4741 gaggccgaac ttgtccttga gccaaccgca cggtccctct tcaccgtcct ttgccagttc
     4801 gctccagaag tggtcgatct cttgctgcgt ttcgcagtgg acctggaacg acacggcctc
     4861 gttgaacttg aaatgcgggc cgccgttgag cgccaggaat ttctgccctt cgagttcgaa
     4921 ctccacagtc atgaccgttc ccgccttctt gccgtgaacc tcgaatccct cttttccata
     4981 acggctgatc ttgccgaccg tcgaattctt gaacacggaa gcgtagaatt tcgccgcctc
     5041 ctcggcctgc gtatcgaacc acaggcaggg cgtgattttt tttgccgtga gctccatgat
     5101 ctactccttt cacgtccctt gcccttccgc tcgtccccgc gaaagcgggg acccagcttc
     5161 gatcctggat tcccgctttc gcgggaatga acggagttca gctgtgcggt ctgagtgtct
     5221 agcctaaaac tgacaactac cgagccagtt ccccgccttt ttcatccgat tccgcttgcg
     5281 accaccccgg taatgcgatt gacacaatgc gttgatatca acataattgt ggcgtgtcaa
     5341 aggcagccga agttcccttc gccaccacgc tcttggtcag ggacacgtgc ctgtgccttc
     5401 acctgcagcg cgcggcgcgg gcgctcgcgc ggcgtttcga cgacgcgctg cggccgctcg
     5461 gcctcaccag cgggcagtat tcgacgatga tgtcgctgaa ccggcccgag ccgcccagca
     5521 tcggttcggt cgcgtccctg ctcgccatgg accgcacgac gctcaccgcc gcgctcaagc
     5581 cattgcaacg gcggggcctg atcacggtga tggtcgacgc caaggaccga cgcagccgcc
     5641 ggctcaagct gacgcccgcg ggccgcgcat tgctcgcgcg cgcggttccg gtctggcggc
     5701 gcacccatgg cgagatcgaa cgcctcgttc cacgtgccgg cgccgatcgc ctgcggcggg
     5761 acctgcgcgc gctgagctaa atccatatct cgatgccgtg ggtgaagggt tttcgagttg
     5821 actccggcga ctgcgagaca tagcagtttt gccctcaccg cgcgccgtct tctccccagc
     5881 gactggtttt ctacgcttcg tgcggagcgc ggcagccgcg gattgcaccg ctccacgccg
     5941 gagagatgca cggcatgcac gacgccaacg ctttgatcac gcggctcccc aaatgcgagc
     6001 tgcacattca cgtcgaaggg agcctcgagc cggagctgat gtttgcgttg gcgcgccgca
     6061 acggcatctc actgccgtat gcgtcggtcg acgccgtgcg gcaggcctac cggttcggca
     6121 acctgcagga tttcctcgac ctctactatc gcggcatgtc ggtgctgatc acggagcagg
     6181 atttctacga cctcgccttc gcctatctcg cgcgcgccca cgccgacaat gtccgccacg
     6241 tcgagatgtt cttcgacccg caaggccaca cgtcccgcgg cgtcgcgttc gcgaccgtgg
     6301 tcgaaggtct cacccgcgcc attgccgacg ccaggcgaga gctgggcgtg gaggcaagcc
     6361 tgatcatgtg cttcctgcgc catctcgacg aggccgacgc cgagcgcacc ctcgacgcgg
     6421 cgctggcgtt tcgcgaccgc atcgtcggcg tcggcctcga ttcgtccgag cggggcaatc
     6481 cgccgggcaa gttcacgcgc gtgttcgcgc gcgcccgcga cgcgggcttc tttctcaccg
     6541 cccatgccgg cgaggagggg ccggcgagct acgtctggga ggcgctcgat ctgctcggcg
     6601 tcgcgcgcat cgatcacggc gtacgcgcca tggaagacca ggctctggtg ggcaggcttg
     6661 cgcgcgagca agtcccgctc accgtctgtc cgctgtcgaa cgtgcggctg cgggtggtcg
     6721 acgacctcgc gcatcacccg ctgcggcgga tgctggacaa agggcttatg gtcacggtca
     6781 attccgacga tcccgcctat ttcggcggct atgtgaacga gaactaccgc gccagcgcgc
     6841 aggcattggg gctcgcacgc gacgagatcg cagcgctggt gcgcaacggg atcagggcct
     6901 cgctgatgac gacgccggag aaggacaagg cgcttgcgga cgtcgatcgc gtcctcgccg
     6961 ggaccgtgtg acggcagagt tacgcagccg cgctgcgtgc catgccgcgg cgatcgcggc
     7021 tgacgacgaa cgcctatggg gatgcgcgca catcctcgcc ccgcagcagg agccggaaca
     7081 tcagcccgtg cgtgatcagg agcagcggca ccacgacggt cggaatgaag taggcggcac
     7141 ccaattgacc cggtgcaaac ccgacggaat tgccctgata gaaagcgtag agcagatcgg
     7201 cggtgcccca caggttgaac acccagacca agggcatccc ccacctgcgc tgcaaggagg
     7261 ccaacgcgag cagcgccagc accgcggcga tgagatcacc gtaggcagcc gggcgcgcaa
     7321 aagcggctga cagatccggc gcgaccacgc cgggcaccaa gaatgccaag ccgacaaagc
     7381 ggaagctgtg caagatgagg agcgggcgca gcgcgtcgat gcgcggctgg ctgcgcaagt
     7441 atggccacag gtatcgcgca gcgacgattc cccacgcaag gaagccc
//
LOCUS       DATLJT010000175         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_8386,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000175 DATLJT010000000
VERSION     DATLJT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8386"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..718)
                     /locus_tag="VM910_03935"
     CDS             complement(38..718)
                     /locus_tag="VM910_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI61734.1"
                     /translation="MALTRDNPSLLSGLPQHLSASLFGNTTPVRLKADEVLFLAGDAG
                     DGCYRVEDGLLKVTMVSRAGTERILAFLGSGAIVGELSLIDGLPRSASVVAVRPAQLG
                     FLSRAAFEEFANKHPEVYKSLLTLIAARLRETDATVAAGSFLPLRGRVACTLLELAQE
                     FGHDVGAGRVVIRQKIGQSDLAAMAGIARENVSRILNDWKRRKLVSRLSGYYCLENKA
                     LLQHEAEL"
     gene            complement(1113..>1628)
                     /locus_tag="VM910_03940"
     CDS             complement(1113..>1628)
                     /locus_tag="VM910_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HVI61735.1"
                     /translation="EKGTVAVMASGPRADVEAVEPALKVIGKFFYIGERPGAGQTMKL
                     CNNVLSAAAMAATSESMVMGVKAGLDPRIMLDVINASSGRSTATEQKFPEVVLPRSFN
                     QGFTAGLMLKDVNLFISEAKTLGIPIQVIEAVAVLLKLTCDELGPDKDITQVVQPIEA
                     RAGVEVRAAKR"
BASE COUNT          322 a          492 c          497 g          317 t
ORIGIN      
        1 aagatcattt cgctttagcg cggacgtggc aatgaaccta gagctcggcc tcgtgctgca
       61 gcagcgcttt gttctccagg caatagtagc cggacaggcg gctgaccagc ttgcgccgct
      121 tccagtcatt gaggatgcgg ctgacattct cacgggcaat gccggccatt gcggcaagat
      181 cgctttgccc gatcttttgg cggatcacaa cgcggccagc gccaacatcg tgaccgaatt
      241 cctgcgccag ttcgagcagc gtgcaggcaa cgcggccgcg cagcggcaaa aagcttccgg
      301 ccgccacagt cgcgtcggtt tcacgcaatc gcgccgcgat caatgtcagc agcgacttgt
      361 agacttccgg atgtttgttg gcgaactcct cgaacgcggc gcgactgaga aagcccaact
      421 gcgccgggcg caccgccacc acggacgccg agcgcggcaa tccgtcgata agcgacagtt
      481 cgccgacgat ggcgccggag ccgagaaaag cgaggatgcg ctcagtgccg gcccgcgaca
      541 ccatcgtgac cttaagcagt ccatcctcga cgcgatagca gccgtcgccg gcgtcgccag
      601 cgaggaacaa gacctcgtcg gccttaagcc ggaccggcgt tgtgttgccg aacaggctcg
      661 ccgaaagatg ctggggcaat cccgacagca gacttggatt gtcgcgggtt agagccatgg
      721 cgcgtctcgc ggctcatttc ggccgggatc ccatcccttt cgctttcgcg gagcatgcgg
      781 cagatttgcg caaaggaaaa gcccgcccgg atggttagtc cggagcgggc gaggttgaac
      841 ccgtggcagg gttcttgggg tgtatggggg acgtgcatca ccccttggat atcatgcggg
      901 tcattggagg tttggggacg ggggaaaccc gctgacatcg aaccgaattg caatcaccgt
      961 ttggcgccag tctttgttat ttttcgattg gcaatctagc gcctcgcgca aaccgcgaag
     1021 tgaccggggt cacagaacgt cgcttttcag taagatattg atataaatag attaattgcc
     1081 cacaggcgcg gctttcgact ggccaaagga gatcagcgct tggccgcgcg cacttcgacg
     1141 ccggcgcggg cttcgatcgg ctgcacgacc tgggtgatgt ccttgtcggg accgagttcg
     1201 tcgcaggtga gtttcaacag caccgccacc gcttcgatca cctggatcgg aatgccgagc
     1261 gtcttggcct cggaaataaa aaggttcacg tccttgagca tcaggcccgc ggtaaagccc
     1321 tgattgaagc tgcgcggcaa aaccacttcg ggaaatttct gctcagttgc agtactgcgg
     1381 cccgagctcg cattgatcac atcgagcatg atgcgcggat cgaggccggc tttgacgccc
     1441 atgaccatcg actccgaagt tgccgccata gctgccgcgg acagcacatt gttgcagagc
     1501 ttcatggtct ggccggctcc cggccgttcg ccgatgtaga agaatttgcc gatcaccttg
     1561 agcgcgggct cgaccgcctc gacatccgcg cgcggaccgg acgccatcac cgcgaccgtg
     1621 cctttttc
//
LOCUS       DATLJT010000176        15955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_697161,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000176 DATLJT010000000
VERSION     DATLJT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697161"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1282..2262)
                     /locus_tag="VM910_03945"
     CDS             complement(1282..2262)
                     /locus_tag="VM910_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVI61736.1"
                     /translation="MSKNKVIITIAPTGGVAFKQQSRYLPTQPAEIARDTYDCYNAGA
                     SVVAVHARRPDDGATCDPVIYRDINTRIRAKCDIIINNSTGGGVHGDMIREAGDGYWE
                     LLWEERLKGMDAGAEMCTLDATTIVASHGGKEIVMNTSPERSKFLAAEMKKRGIKPEW
                     EVFSPTHIVQDVASLTTAGFDEEPFYVNIVLGVHRSFQNAMPYSPRILQSMVDLLPKG
                     SIFGVSGIGPAQLPCAMNALLLGGHVRVGLEDNLYYSPGELTTNQKLTQRAVRLVREM
                     GYEPATPAEARLILGLPRKGAARPEFGIDGPGNRTQATGDEARKPIPSAA"
     gene            complement(2335..3993)
                     /locus_tag="VM910_03950"
     CDS             complement(2335..3993)
                     /locus_tag="VM910_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI61737.1"
                     /translation="MVPSAHVDSFARDNLPPRQEWPDFLCDLPGLNYPERLNATVELL
                     DRKVEAGHGERPCLMSATESLTYRQLAERVNRIANVLSRDLGMVPGNRVLLRAPNSPM
                     MVAAYLAVIKAGGVAVGTMPLLRARELAYPITKAKIALALCDVRLADELEKARSLAPN
                     LARVAYWGSGASDSLETLMMQPGYESFAACDTANDDVCLIGFTSGTTGEPKGTMHFHR
                     DLLAVCDTYARHVLRPTAEDRFIGSPPIAFVFGLGGLVLFPLRVGASSILLEKASPEE
                     LLPAISKWRATVLFTAPTAYRALLPMLANHDVSSLRKCVSAGETLPEATFEAWREATG
                     LTIMDGIGSTEMLHIFIGSPDEDVRAGATGKVIPGYEARVVDGEGREVPPGTIGRLAV
                     RGPTGCRYLADPRQRKYVQNGWNVTGDTYVMDDEGYFWYQARSDDMIISAGYNIAGPE
                     VEAALLTHPAVAECGVVGAPDEKRGQIVRAYVVLRPPYAGDAALTRALQDHVKATIAP
                     YKYPRAIEYVSSVPRTETGKLQRFELRRRAAVTTSTGSSRCSRN"
     gene            complement(4242..5588)
                     /locus_tag="VM910_03955"
     CDS             complement(4242..5588)
                     /locus_tag="VM910_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61738.1"
                     /translation="MSTKHTIDVQNFINSHTLSRFQKLVIALCFAVVAIDGFDTAAVG
                     FVAPALKMQWSLTPAQLAPLFGSGLFGLMIGAFIFGPMADKIGRKAVLVATTLFFGLA
                     SIACAFSASIEMLTVLRFVTGLGLGGAMPAAITLTSEFCPERERSSLVTLMFCGFTSG
                     SAAAGLLGSHVVTAYGWKGLLVLGGILPIALAPVLWALLPESARYLTLKGARSEMIAA
                     VLQRVAPEADLQDAIFSGVRQPKGSPMLQLFEKGLATGTLLIWLTFFMSLLVFYLLSS
                     WLPLVITNAGFSLANASLMAATLATGGTVGAIVIGRLMDRFDPRLVLAVSYVLAGVFV
                     VLLGSATGSPRLLVFAIFGAGFGAAGAQVGVNALAATYYPTASRATGVSWANAFGRIG
                     SVLGSTVGGFLLSFGWDLATVFSVAAIPAFVAGAAMLAKAWLYASAPIIAKPVAAG"
     gene            complement(5567..6229)
                     /locus_tag="VM910_03960"
     CDS             complement(5567..6229)
                     /locus_tag="VM910_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI61739.1"
                     /translation="MTAIALDERLDIRGPPEAAAATIPAGQTAVGLAGLRMRQLGASD
                     LPAVERHLVQLGPLDRRARFLCYRPDAAIAAYVRRIDPSSTVLMGAFDPSDRMVGLAE
                     AHPIDSPHTVEVAVSIDPPFRRCGLGQRLVARALAVAFDRGMQWAEFIFAPNNRALAG
                     LVETLGGRIKAPGHALIDRSQIVWNARQPEARRRLVGEIGLIISTTKSNKENFACPRN
                     TQ"
     gene            complement(6277..6468)
                     /locus_tag="VM910_03965"
     CDS             complement(6277..6468)
                     /locus_tag="VM910_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61740.1"
                     /translation="MRTRVLLAAVMMLTALGAALAIKSFTPDTLAVAAPQPVMISIDG
                     LHRQVDVRSLPELEIKDPY"
     gene            complement(6826..7923)
                     /locus_tag="VM910_03970"
     CDS             complement(6826..7923)
                     /locus_tag="VM910_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI61741.1"
                     /translation="MQEETEMHGTLTQSLKLERRVPSRPKPGKTGREKVVNLALQGGG
                     SHGAFTWGVLDRLLEEKRLTIEGITATSAGAVNAVLVAHGLATGGNEEARSALRNFWQ
                     RISAAASHGVFQPSLIDKMNGNFGLEHSPGYLLMGALSCFASPYQLNPFDYNPLKNLL
                     EEIVDFARLREQPVVKLFLCATNARTGKPKIFKGDELQLDHVLASACLPLLTRAVEID
                     GEHFWDGGFAGNPAIFPVIYRCEAPDVIMVHLTPTERPDLPTTSYEIINRMQEISLNS
                     SFMGEMRSLALITKLIDDGRVAGGKRMLMHLIDAEDVIREFPGSSRLNCDWDFLLHLH
                     DIGRQRADQWLASNFDNLGVRSTVDLRAKYF"
     gene            complement(7963..8367)
                     /locus_tag="VM910_03975"
     CDS             complement(7963..8367)
                     /locus_tag="VM910_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI61742.1"
                     /translation="MQPASWRVGSRGIFVGGGGAVRLPRLIGTARMMAMMLTGRIYEA
                     EEGQAIGISHYLVPPGAGLAKGIEIAKRIASNSPISNFAITHALPRIAQSDSAAGYLT
                     EALIAAIVRHSDEAKARLKSFREKRAPKTVRN"
     gene            8433..8576
                     /locus_tag="VM910_03980"
     CDS             8433..8576
                     /locus_tag="VM910_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61743.1"
                     /translation="MIKPSRFTSVPSTPSTRWFVGIAFAAIGIGALVIFGFSVPGYIA
                     LLN"
     gene            complement(8649..8879)
                     /locus_tag="VM910_03985"
     CDS             complement(8649..8879)
                     /locus_tag="VM910_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61744.1"
                     /translation="MTRLRALEIEAYVLSTMGGAIDAPPPALRSVRKFATPSQTLSRA
                     QAVTKWIAIALLLAGAGLELVLCFTVGSSGPL"
     gene            complement(9613..12069)
                     /locus_tag="VM910_03990"
     CDS             complement(9613..12069)
                     /locus_tag="VM910_03990"
                     /inference="COORDINATES: protein motif:HMM:NF010411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system VirA-like sensor kinase"
                     /protein_id="HVI61745.1"
                     /translation="MRPVLGAVGVLLLLGLLTWLLLRGIDTNAPAYEATLQAFDDFAL
                     AEASLHRDVLQARAGLLRDYDSVGTAIEAMEGAVARLRSHAERESLDVGPVDRLAATV
                     AQHEDLTESFKTSNALLQNSLSYVGLLSTDSVFGARDAALGPQAGALAAAILHLARDT
                     SSGTVAALQEQIDRFDAQKAAAGPAAEAGRALLAHARLLQGLLPSVDATLRSLVSVPS
                     REALEATRTLFANRQAALEAMAKRYRLLLYLTSLLLVVVLVRLGLRLRVRALALRQRA
                     AFEHVIAENSTRLINCSPAETAALLDTVLAELARAIGTERAYVVIEGSPARVHAWCAE
                     GAKYPPDWPVQALALFRKLGSAGTDILTVPDVTALPPGKAKKTLEAAGVRGWACAPLI
                     RPGRVQGVMGFDTFQPAWDKIFPLPVVRLAGDAMVNAIEREFLERDRATLATRLERAR
                     RMQMIGSLASGIAHNFNNIIGAILGYSEMVEPQLAPGSKSAGHIDEIRRAAERGRDLI
                     DNILTFGRRRDTRVQSVRVRTLFEEAASLLRGSLPASVELIVADVPADIVLSGEPAQL
                     QQVILNLCTNAAQAMEDGGSIQVTGESKDVAVPLALSHGELAPGRFVCLSVADTGRGF
                     DEGVAQRLFEPFFTTRKAGTGLGLATVHEIVRDHDGAMNVQSKPGHGSRFEAWLPALA
                     DVAAAPTRALPLGRGETVLIVESERLLRDEEMLAALGYEPVGFERADDAIAACRSAPD
                     RFDAVVIGHIAADVNSLHVAWVLHGLMPRHPILLASASTIGVSVDELGEAGISEVLPR
                     PLVSTELAAALARSLRSLGN"
     gene            complement(12066..13019)
                     /locus_tag="VM910_03995"
     CDS             complement(12066..13019)
                     /locus_tag="VM910_03995"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVI61746.1"
                     /translation="MAGLLLVLVCLGGVSPTAAQLPTLEPRVVREPITPIPATPAQDQ
                     RRVALGEQLFHDRRLSHDNTRSCSSCHDTRTNGASAKARDSNPHGEPLALNTPTVFNV
                     GLNFRLNWEGNFRSLERQAEQALSKPAVMASSVEEVLAKLRADPDVVKQFRDAYGREP
                     DRDSLLDAIATFERSLTTPGSRFDRWLVGDGGAITPEELSGYQLFKSLGCVSCHQGVN
                     VGGNLFQRHGVFHPLGSPEPEVVRVPSLRNVATTAPYFHDGSAATLAEAVKAMGITQL
                     DRALTNEQIAAVVAFLRTLTGTYNGMPVTPGAEGPHAAVPR"
     gene            13073..13834
                     /locus_tag="VM910_04000"
     CDS             13073..13834
                     /locus_tag="VM910_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI61747.1"
                     /translation="MGKTSSNDPGLRASSDRQVCVLVVEDDPALQRMILSYFGENNIR
                     TLVASDREEMVRQLNDAVVNLLILDLRLGQEDGLDLLREVRSSSDVPVIIITGHRRDD
                     IDRVVGLELGADDYLTKPFNLRELLARVRAVLRRFDSGRAPVREPERGRWRFSGWQLD
                     RRTRRLTDPDGAPVVLTKGEYALLVAFLEAPQRPLSREHLLQATRVHEDVFDRSIDVQ
                     ILRLRRKLERDPSAPRVIQTERGVGYVFAVPVERV"
     gene            complement(13919..15607)
                     /locus_tag="VM910_04005"
     CDS             complement(13919..15607)
                     /locus_tag="VM910_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61748.1"
                     /translation="MSTIAAGLPRREESGMTAEERLVILASSLGTVFEWYDFYIYGTL
                     GVFLAKYFFSNVAPAVGFIFTLLAFAAGFAVRPFGALLFGRTGDLVGRKYTFLITMTL
                     MGLSTFLIGVLPSYATAGIIAPIILIGLRLVQGLALGGEYGGAAIFVAEHAPPGKRGY
                     YTSWIQTTATLGLFMALLIILGIRTWMGEEAFGEWGWRLPFLFSIMLLAVSIWIRLKL
                     QESPIFQRMKEQGEGSEAPLTEAFGRWQNAKMAILALLGATAGEAVIWYGGQFYALLF
                     LTETLKVPGATAQILVAIALAIGTPFFILFGWLSDKIGRQPIMLLGFFLAVVTYFPLF
                     QGITHFANPALETALAEAPVVVIADPAECHFQFNPVGTAAFTSPCDIAKAALVDRSVN
                     YRNEAAPPMTPASVRVGKQIVPTTAPDFARVLNDAITAHGYPATADPSRINAVMTVIL
                     LTILVIYVTMVYGPIAALLVELFPTRIRYSGVSLPYHIGNGWFGGFLPATAFAIVAAT
                     GDIYSGLWYPIVVAAMSFVVGLIFLPETKDRDIAYLWRLPPNLSIDPFEDFRLL"
BASE COUNT         3136 a         4853 c         4948 g         3018 t
ORIGIN      
        1 catggttttg atcgtgttgg tatgctcgag cttgtcttgt ctcgggatca ataaagcaag
       61 aactttcaag tagatcccag gctggtcgcg gcgcaccttc gcaatggcct tctccccgtg
      121 cttggagaaa tccctcagca atgcgcaaat gacctcctcg ctcagcttgt tacgcgagcc
      181 ccgcagtcgt ccgcggacga gctgaaacgg aagcgaaact gcgcaaccgc tcaagctcag
      241 ccgatccggc tgctcaccca gtccggccgc gctggcgtcc acccggcccc tgaccgaagt
      301 cgatcgacga cgcgagggaa ttgaaggggg cgggctgatc gacggggcac tggaaactgg
      361 tttgcaagta gtcgatccac aaaagatcgg tgcagcggct tccctcccac agcggctccg
      421 gcattgcctt cggcttcgaa catagaggga tacccatcgc gggctggata atacggcgtc
      481 attggcgtaa ctcctttctg cccctttgct gagacagccg cgaagatggc gccgctgcgc
      541 tacagaagaa tttcagagtt ggggaactgc gttacgttcg taaccgtgga ccgtcaccat
      601 gcgtgtcctg cgacgcgcgt cagcagatcc ggccaccgtc aacgcaatgg gcggccagcg
      661 cctcgaggcc gtgaggcttc aatcagaatc aatttcgcgt ctgcttggag ggcagcgtaa
      721 gatcgccaag gagaccggcc ggagccggca cgccttggcg agtttagagg tggagtccgt
      781 tagcggctcg aaaatacatg ccattttgcg gtcgtccaac gcaccgggcg gtgcaaaaac
      841 gctacagctg tatccacggc gcgtgcataa gctccaagcc ggtgaacttt gcgtatgcag
      901 ttcgccatgc gtgttgcaat tccgtacccg cgactgtcga cttggcgtgc ggaaccaact
      961 gcccattcac acggatcagg ctcacgcctg agactgccgg cgcggtatct tgctggcagc
     1021 ggctgttatc gtcactcatg atcggttctc cttggtttat gtaacagcac caaaatggcg
     1081 gcgctgcgct acagaagaat ttcggatttg ggaaatcgcg ttactttcgt aacggcgcat
     1141 cccccaccct gtgaacggag caaaggtatc taatcgcgaa gtcatggcgc tcgcggccgc
     1201 cgatcacgtc gcccatcacg gcgatctcct cgggatgacg tcgaaggtga tcggcgccgc
     1261 aggttgcggc ggcgccatgc cctaggcggc cgatggaatt ggcttcctgg cttcgtcccc
     1321 cgtcgcctgc gtccgattgc cgggcccatc gatgccaaac tcggggcgcg ctgcgccctt
     1381 gcgcggcaag ccgaggatca gcctcgcttc ggccggcgtc gcaggctcat aacccatctc
     1441 gcgcacaaga cggacggcgc gctgggtcag cttttgattc gtggtaagct cgcctgggct
     1501 gtaataaaga ttgtcctcga gaccgacgcg aacgtgacca ccgagcagca gagcgttcat
     1561 cgcgcatggg agctgagcag gaccgatgcc gctgacgccg aaaatgcttc ctttgggcag
     1621 gagatcgacc atcgattgga ggatgcgcgg cgaatacggc atagcattct gaaagctgcg
     1681 atgcacgccc agcacgatgt tgacgtaaaa cggctcctcg tcgaagccgg cggtggtcag
     1741 gctggccacg tcctggacga tgtgggttgg gctgaagact tcccattcgg gcttgatgcc
     1801 acgcttcttc atctccgcgg ccaggaattt cgaacgttca ggcgaagtgt tcatgacgat
     1861 ctctttaccg ccatggctgg cgacgatcgt cgtagcatcg agggtgcaca tctctgcgcc
     1921 cgcatccatg cccttgagac gctcctccca gaggagctcc caatagccat cgccagcctc
     1981 tcggatcatg tcgccgtgga caccgccgcc agtggaattg ttgatgatga tgtcgcactt
     2041 ggcacgaatg cgcgtattga tatcgcgata gatgaccgga tcgcacgttg cgccgtcgtc
     2101 gggccgccgc gcgtgcacgg cgaccacact ggcaccggcg ttgtaacagt cgtaggtgtc
     2161 gcgcgcgatc tcggctggct gggtcggcag ataccggctc tgctgtttga acgctacgcc
     2221 gccagtcgga gcgattgtga tgatgacctt gttcttgctc atttggactt cctcttatgg
     2281 gctctcgggt ttggaaaagc tggggggcct tacggcaaca ccgcctgcga cggctcaatt
     2341 gcgtgagcaa cggcttgagc ctgtgctcgt ggttaccgct gcacgtctgc gcagctcgaa
     2401 gcgttgaagc ttcccggtct ccgtgcgcgg cacgctgctg acgtattcga tcgcacgcgg
     2461 atatttgtag ggcgcgatcg tcgccttgac atgatcctgg agcgcgcgcg tcagcgcggc
     2521 atcgccggca tagggaggcc gcagcaccac gtaggccctc acgatttggc cgcgcttctc
     2581 gtcgggtgcg ccgacgacgc cgcattcggc gaccgctgga tgcgtgagca gggcggcttc
     2641 gacttccggc ccggcgatgt tgtagccggc tgaaatgatc atgtcgtctg aacgcgcctg
     2701 gtaccagaag taaccttcgt catccatgac gtacgtgtcg ccggtcacat tccagccgtt
     2761 ctggacgtat tttcgttgcc gtgggtcagc gagatagcgg cagccggttg gcccccgcac
     2821 cgctagccgg ccgatggttc cgggcggcac ctcgcgtccc tcgccgtcca caacgcgcgc
     2881 ttcatagccg ggaatgacct tgccggtcgc ccctgcgcga acatcctcat cgggtgatcc
     2941 gatgaagatg tgcagcattt cggtcgagcc gatcccatcc atgatcgtca ggccggtggc
     3001 ctcgcgccat gcctcgaagg tcgcctcggg caaggtctcg ccggcggata cgcacttgcg
     3061 cagcgacgag acatcgtggt tggcgagcat cggcagcaag gctcggtacg cggtcggcgc
     3121 ggtaaacagc acggtcgcgc gccacttcga gatcgccggc agcaactctt cgggcgatgc
     3181 cttttcgagc agaatcgagg acgcgcctac tcgcagcgga aacagcacca gtccgccgag
     3241 gccgaacacg aaagcgattg gcggcgagcc gatgaaccga tcttcagcgg tcggccgcaa
     3301 tacgtggcgg gcgtaggtgt cgcaaactgc gagcaaatcg cggtgaaaat gcatagttcc
     3361 cttgggctcg ccggtcgtgc ctgatgtgaa gccgatcaag cacacgtcgt cattcgccgt
     3421 gtcgcaggcc gcgaagctct cgtagcccgg ctgcatcatc aatgtttcga gggagtcgga
     3481 cgcgccgctg ccccaatagg ccacacgtgc aagattcggc gccagcgatc tcgccttctc
     3541 gagctcgtca gcgagccgca cgtcgcacag cgcgagcgcg attttcgcct tcgtgatcgg
     3601 atacgcaagc tcgcgggcac gcaacagcgg catggtgccg accgccaccc cgccggcctt
     3661 gatgaccgcc agatacgcgg caaccatcat cgggctgttg ggcgcgcgca gcagcacgcg
     3721 atttcccggc accatcccga ggtcgcggct gagcacattg gcgatgcggt tcacgcgctc
     3781 ggcgagttgc cgataggtca ggctctcggt cgccgacata aggcacgggc gctcgccatg
     3841 tcccgcttcg accttgcgat cgagcagctc caccgtggca ttgaggcgct ccggataatt
     3901 gagccccggt agatcgcaca agaagtcggg ccattcctgg cgcggaggca gattgtcgcg
     3961 cgcaaaacta tccacgtgtg ccgagggaac cattggatgt gctcctgcgg gagcaaatct
     4021 tgccttcggg caccgcgcct ggagtgttag actctcactt acggttgagg cgtcgagcat
     4081 caggctgact tcggcgccgc gcgatccgcg atgagagccg tcgaggacgt gcccgccgct
     4141 cgcggggttg tcctcgaccg ccgtgtcgat tgggttgtcg atcgtattga gcattgattc
     4201 tctccttctc tcactgctgc gcggtttctc gcgtagagcg cctatccggc cgcgaccggc
     4261 ttggcgatga tcggtgcgga cgcgtagagc caagccttgg cgagcatggc ggcgccggcg
     4321 acgaaggctg gaatggctgc gacgctgaac acagtggcca aatcccagcc gaacgagagc
     4381 aggaatcctc cgaccgtcga gcccagcact gagccgatgc ggccgaacgc attggcccag
     4441 ctcacgccgg tggcacggct cgcagtcgga tagtacgtgg ctgctagagc gttgacaccg
     4501 acctgagcgc cggcagcgcc gaagcctgcg ccgaaaatcg cgaagacgag cagtctgggt
     4561 gaccccgtcg cgcttccaag caagaccaca aagacgccgg cgagcacata ggacacggcg
     4621 agcaccaggc gaggatcgaa gcgatccatc agccggccga tcacgattgc gcccaccgtg
     4681 ccgccggtcg cgagcgttgc cgccatgaga gaggcgttgg cgagcgagaa acccgcgttc
     4741 gtgatcacca gcggcagcca gctcgacagc aggtagaaca ccagcaggct catgaagaaa
     4801 gtgagccaga tgagcagcgt tcccgttgcg agccctttct cgaagagctg caacatgggc
     4861 gagcccttcg gctggcgcac gccggaaaag atcgcatctt gcaggtcggc ctccggtgct
     4921 acgcgttgca acactgcggc gatcatctca gagcgtgcgc ccttcagtgt caggtagcgc
     4981 gctgactccg ggagcagggc ccacagcact ggggccagcg caatcggcag tatgccgccc
     5041 agcaccaaca gacctttcca gccataggcg gtaacgacgt gcgaccctag aaggccagcg
     5101 gcagccgaac cggaggtgaa accacagaac atgagcgtca ccagcgacga gcgctcacgc
     5161 tccggacaga attccgaggt gagagtaatt gcggctggca tggcgccgcc gaggccgagc
     5221 ccggtgacga agcgcagcac tgtcagcatc tcgatcgagg cggagaaggc gcacgcgatg
     5281 ctcgcgaggc cgaagaacag cgttgtcgca acgaggacgg ccttgcgccc gattttgtcg
     5341 gccatcgggc cgaaaataaa ggcgccgatc atcagaccga acagtccgct gccgaacagt
     5401 ggggcaagct gcgccggcgt caggctccat tgcatcttga gcgccggggc gacaaagccg
     5461 accgcggccg tgtcgaaacc gtcgatcgct acgaccgcga agcaaagcgc gattacgagc
     5521 ttctggaagc gcgatagggt atgcgagtta ataaagttct ggacatctat tgtgtgtttc
     5581 gtggacatgc gaagttctcc ttgtttgatt ttgtggtgct gatgattagt ccgatctcac
     5641 ccacaaggcg tcgtcgggcc tcaggctgcc tcgcgttcca cacgatctgg gagcggtcga
     5701 tcaaggcgtg gccaggggct ttgatccggc ccccgagggt ctcgacgagg ccggccagcg
     5761 cgcggttgtt gggagcgaag atgaattccg cccactgcat gccgcggtca aacgccaccg
     5821 ccagagctcg ggcgacgagc cgctgcccca ggccacaccg ccggaacggc ggatcgattg
     5881 acactgcgac ctccacggtg tgcggcgagt cgatcgggtg cgcctcggcc agtccgacca
     5941 tgcggtcgct ggggtcgaag gcgcccatga gaaccgtaga ggacggatcg atcctgcgta
     6001 cgtatgcggc gatcgccgcg tcgggacggt agcagagaaa gcgggcgcgc cgatccaacg
     6061 gcccgagctg gaccaggtgg cgttcaacag cgggcaggtc gcttgcgccg agctggcgca
     6121 tacgaagccc ggcgagtccg actgcagttt gtcccgccgg gatcgttgca gctgccgctt
     6181 cgggaggccc tcggatgtcg aggcgctcat caagcgcgat ggcggtcatg gcactattct
     6241 ccttgcgcgc cggctggcgc ttgatagcct gttgcctcaa taggggtcct taatttccag
     6301 ctcagggagc gatctcacgt ccacctgacg gtgaagccca tcgattgaga tcatcacggg
     6361 ctgcggcgcg gccaccgcca gcgtgtcagg tgtgaacgac ttgatcgcca aggcggcgcc
     6421 cagcgccgtc aacatcatca ctgcagcgag aagaacgcgt gttctcatgg aaacctcctg
     6481 tttgctgacc taagaaatga cgtcgccgcg ctacagaagg atttcagctg gcgggaattg
     6541 cgttacgtcc gtaactcgct tggcgcatga caggatcgca ggccagcgtc agggggaggg
     6601 tccgcgtcgg ttggcttccg gcggagctca agttgcgcgc gcattcgatt attccagcat
     6661 cgaacagcgc gcgacgcctc gcgagtcggg catcatggca gtcacgctgc ggaaactcgt
     6721 ggtttggcgt ccgacgagaa tgtcgcggca ctacaaaaga atctcagctg taaagaatcg
     6781 cgctacgttc gtaacgagcg ggcgcttggt ttgcgctgcg acgaatcaaa agtacttggc
     6841 ccggagatcg acggttgacc gcacaccgag attgtcgaaa ttggaagcca gccattgatc
     6901 ggcacgttgg cgaccgatgt catgaagatg cagcagaaaa tcccaatcac aatttaatct
     6961 gctggagccc ggaaattctc tgatcacgtc ctcggcatcg atcaagtgca tcagcattcg
     7021 ctttccaccg gccactctac cgtcgtcgat aagcttggtt atcaacgcca gggagcgcat
     7081 ctctcccatg aaagaggaat tgaggctgat ttcctgcatc cgatttataa tttcatacga
     7141 ggtcgtcgga aggtccggtc gctcggtcgg cgtgagatga accatgatga cgtcgggagc
     7201 ctcgcatctg taaatcaccg gaaaaatcgc aggatttccg gcgaacccgc cgtcccaaaa
     7261 atgctcgccg tcgatctcaa cagcacgcgt caggagcggc aggcatgccg acgcaagcac
     7321 gtggtcaagc tgaagttcat cacctttgaa gattttcggc ttgccggtcc gcgcgttggt
     7381 cgcacagaga aatagcttca ctaccggttg ctcgcgcagc cgtgcgaaat cgacaatctc
     7441 ctcaagcagg ttcttgagcg gattgtagtc gaacggattg agctgatagg gcgacgcaaa
     7501 acaactaagc gcccccatca agaggtaacc gggcgaatgt tcgagaccga aattgccatt
     7561 catcttgtca atcaaagatg gttggaagac gccatgcgat gcagcggcgg atatcctttg
     7621 ccagaaattc ctgagcgcgc tcctcgcctc ttcattacca ccggttgcca aaccatgggc
     7681 gactaggacg gcattgactg cgccggcgct cgtcgcagtg atgccctcga tggtcaaccg
     7741 tttttcctcg agtagtcgat cgagcacacc ccatgtgaat gcgccgtggc tacctccccc
     7801 ttggagcgcc aagttgacca ccttctccct tccagttttg cccggcttag gccgggacgg
     7861 gacgcggcgt tccaatttaa gggactgcgt aagcgttccg tgcatctcgg tctcctcttg
     7921 caactctggc cttgggtccc atcgatgttg cgtttgtcgc cgtcagttgc gaaccgtctt
     7981 cggcgcccgc ttctcgcgga atgatttgag ccgcgccttg gcctcgtcgc tgtgccgcac
     8041 aatggcggca atcagcgctt cagtcaggta gccggcggcg gagtcggatt gtgcgatccg
     8101 cggcaatgcg tgggtaatag cgaaattgga gatgggcgag ttgctggcta tgcgcttggc
     8161 gatctcgata ccctttgcaa gtccggcgcc aggtgggacg agatagtgcg agatcccaat
     8221 ggcctgacct tcttcggcct catagatacg tccggtaagc atcatggcca tcatccgcgc
     8281 cgtaccgatg agccgcggca ggcgcacagc gccgccgcca ccgacaaaaa tgccgcggct
     8341 tcccacgcgc caagaggcag gctgcatccg attcgagctc cggcagaatc gggaatcccc
     8401 gacggagttt gccgtcgtct cagagtggct gaatgatcaa gccgtccagg ttcacgtcgg
     8461 tgccttctac acccagcacg cgttggttcg tcggcatcgc cttcgcggcc atcggcatcg
     8521 gggcgctggt gatctttggg ttcagcgtgc ccggctacat cgccctcctc aattgacgtt
     8581 gacatgacat tcttccttcg aggttcatcg aattgcgatc ggacgcgtca gcaaatgatc
     8641 ccaggctttc agagagggcc gctggacccg accgtgaaac acaggaccaa ctccagcccg
     8701 gcgccggcca ggagtaatgc gatcgcaatc cacttggtaa cggcttgagc gcggcttagt
     8761 gtttgcgacg gcgtagcgaa cttgcggacc gagcggagcg ctggcggggg cgcgtcgatc
     8821 gcgccaccca tggtcgagag gacataggct tcgatttcaa gcgcacgcag acgagtcatt
     8881 tcaggtctcc tgttggattt gcgcgtctcg ggcgcgctct tcatctctgg cgaagcggtt
     8941 cggcagggtt ctctccaagg cggattgccc gcgtcatcga ggcccatctg ccgccaagtg
     9001 caggccaatc gcccctctgc cgtgtgcccc catcggcaca ccaatggtcg tcgcgagggc
     9061 ttcgtcgatg cgggttcggc ggtctggttc agtgttcgcg agtcgagagc gatgctttcg
     9121 atgtatcggg gatcgaacat cacggcgttc atggcgcggt tctcctcatt cgtgcctgtc
     9181 cgcacgatgg cgatgccgcg ctactgaaga atttcagcat cgggaaattg cgttacgttc
     9241 gtaacgctgc tgggcactgg ctgaccgtgc gcgaagacaa acggcaagct tggctctcac
     9301 ggagtggatg caggatttcg gcaaacggct ctgccgccgt cgtacggtga cactgcgttg
     9361 atcacgaaca agatcttcat tttgaatcat tcctttgttg gcattcgact tgcgccgacg
     9421 cccatttttg accagcgatg ccaagaactg agctcgtcct gccgggacct gcagtttcgc
     9481 gatgaacatg aaccgcccag ctgagcgcgt cgttgtcgtt aagctggcga cgtcgacgcg
     9541 aacgggcgga tgagcggcgc tacagaagcg tttcagcgat ggggaatcgc gttacgatcg
     9601 taacgtggcg gatcaattgc cgagtgagcg cagggaccgc gccagagccg ccgccagctc
     9661 ggtgctgact aggggccggg gcaggacttc ggaaataccg gcttccccta attcatcgac
     9721 gctcacgccg attgtggaag cgctggcaag cagtatgggg tgtcgcggca tcagcccatg
     9781 aaggacccat gcaacgtgga gactattcac atcagccgct atgtgaccga tcaccactgc
     9841 atcgaaccga tcaggcgccg aacgacatgc cgctatcgca tcgtcagcgc gctcgaaccc
     9901 aaccggctcg tacccgagcg ccgccagcat ctcttcgtcg cgcagcaggc gttcgctctc
     9961 gacgatcagc accgtctcgc cgcgcccgag cggcaacgct cttgtaggcg cagcggcgac
    10021 gtcagccaag gcaggcagcc acgcctcgaa gcggctgccg tgtccgggtt tgctctgcac
    10081 gttcatcgca ccgtcatggt cacgaacgat ttcgtgcacg gtcgcaaggc caagcccggt
    10141 tccggccttg cgtgtcgtga agaacggctc aaacaatctt tgtgctacac cttcgtcgaa
    10201 accgcgtccc gtgtcggcaa ccgaaagaca cacgaagcgt cctggcgcca gctcgccgtg
    10261 gctcaacgcg agaggaacag ccacgtcctt cgactcccca gtgacctgga tggatccgcc
    10321 gtcctccatc gcctgcgccg cgttggtgca caaattgagg atgacctgct gcagctgcgc
    10381 cggttctccg gaaagaacaa tgtcagcggg aacgtccgcg acgatgagct caacgctcgc
    10441 aggcagcgac ccgcggagca atgacgcggc ttcctcgaac aacgtacgca cccgcactga
    10501 ctggacgcgg gtgtcacggc gccgcccaaa cgtgaggatg ttgtcgatca gatcgcgccc
    10561 gcgctcggcg gcccgtcgta tttcgtcgat gtgccccgcc gacttgcttc ccggcgcgag
    10621 ttgtggctcg accatctccg aataacccag aatagctccg ataatgttat tgaagttgtg
    10681 cgcaatcccg ctagccaggg atccgatcat ctgcatacgc cgcgcccgtt ccagtcgtgt
    10741 tgcgagcgtt gctcggtcgc gctcaaggaa ctcgcgctca atcgcattga ccattgcgtc
    10801 gccggcaagg cgtactacgg gcagggggaa tatcttgtcc catgccggct ggaaggtatc
    10861 gaaccccatc acgccttgta cccggccggg tcgtatcaac ggcgcgcagg cccagccgcg
    10921 gacaccggcg gcttcgaggg tcttcttggc cttgccaggc ggcagggcgg tgacgtcggg
    10981 cacggtgagg atatcagtcc cggcagagcc tagctttcgg aacagcgcga gcgcctgcac
    11041 cggccaatcc ggcggatact tcgctccttc cgcgcaccag gcatgtacgc gggcagggct
    11101 cccctcgatc accacatagg cgcgttcggt gccaatcgct cgcgcaagct cggcaagaac
    11161 cgtgtccagc agcgccgcgg tttctgccgg cgagcaattg atcaggcgcg tcgagttctc
    11221 ggcgatgaca tgttcgaatg ccgcccgctg tcgtagcgca agggcgcgaa cacgcagccg
    11281 cagaccgaga cgcacgagca cgacgaccaa cagcagcgaa gtgaggtaaa gcaaaagccg
    11341 atagcgcttt gccatcgctt cgagggccgc ctgacgatta gcgaacaacg tgcgggtcgc
    11401 ttccaaagct tcccggctcg gaactgagac gagcgatctg agggttgcgt cgaccgaagg
    11461 caacaggcct tgcaacaaac gcgcatgcgc aagcagcgcc cgtccggctt ccgcggccgg
    11521 gccagccgcg gccttttgcg catcaaatcg gtcgatttgc tcctggagag ccgccaccgt
    11581 ccccgatgac gtgtcgcgcg ccagatggag gatcgccgcc gccaatgcgc cggcttgagg
    11641 accgagcgct gcatccctgg cgccgaacac cgaatcggtg ctcagcaggc caacatagga
    11701 caacgaattc tgcaaaagcg cattgctggt cttgaagctc tcggtcaagt cctcatgttg
    11761 cgcgaccgtc gcggccagac ggtcgactgg tccgacatcg aggctctctc tctccgcatg
    11821 ggatcgcagc cgagcgaccg cgccctccat cgcttcgatt gccgtgccaa cgctatcgta
    11881 atcgcgcagg aggcctgcgc gcgcctgcag gacgtcgcga tggagcgacg cctccgcgag
    11941 cgcgaagtca tcgaaagcct gaagcgtcgc ctcataggcc ggtgcgttcg tgtcgatccc
    12001 tcgcaacagc agccacgtca gcagcccgag cagcagcagt acgccgacag cacccagcac
    12061 cggtctcatc gtggcaccgc cgcgtgcggt ccctcggctc ctggggtgac cggcataccg
    12121 ttgtaggtgc ccgtgagcgt acgcaggaag gcaacgacgg cggcgatctg ttcattcgtc
    12181 agtgcgcgat ccagctgggt gatccccatc gctttgacag cctccgccag tgtcgccgcg
    12241 ctgccgtcat gaaaataggg cgctgtggtc gccacattgc gcaggctggg gacacgcaca
    12301 acctcgggct cgggagagcc tagcggatgg aagactccgt gccgctggaa caggttgccg
    12361 cccacgttta cgccctggtg gcaggagacg cagccaagcg acttgaacag ctgataaccg
    12421 gagagttcct ctggcgtgat cgccccgcca tcgccgacga gccagcgatc gaagcgactt
    12481 ccgggcgtcg tgagcgagcg ctcaaacgtc gcgatcgcat ccaaaagact gtcgcggtcg
    12541 ggctcgcggc cataagcgtc tcggaactgc ttcacaacgt ctgggtccgc gcgcagcttg
    12601 gctaaaactt cctcaacgct cgacgccatg acggccggtt tgctgagtgc ttgctcggcc
    12661 tggcgctcca gcgagcgaaa gttaccctcc cagttcagac ggaagttcag ccctacattg
    12721 aatacggttg gcgtattcag cgccagcggt tcgccatgcg ggttggaatc gcgagctttg
    12781 gcgctggcac cgttggttcg tgtatcatgg cacgatgaac agctgcgcgt gttatcgtgc
    12841 gacaggcggc ggtcgtggaa caattgctcc cctagtgcga cgcgtcgctg gtcctgcgcc
    12901 ggcgtggcag gaattggtgt gataggttca cggaccacgc ggggctcaag ggtcggcaat
    12961 tgagcagccg tgggagagac gccgccgagg cagacgagca ctagcagaag gccggccatc
    13021 cgggggctcg gccgagttgc cgccggcggt ctcaaggacg gaactgggca gcatgggtaa
    13081 gacatcgtca aatgacccgg gccttcgcgc gagcagtgat cgccaggtgt gcgtccttgt
    13141 cgtcgaggat gatcctgctt tgcagcgtat gattctaagc tacttcgggg aaaataacat
    13201 ccgcactctg gtggcgtcgg atcgtgagga gatggtccgc cagctcaatg acgctgtcgt
    13261 aaacctgctg atcctggacc ttcggcttgg ccaggaggat ggccttgatc tcctccgcga
    13321 ggttcgctcg agctccgatg tgccggtcat tatcatcacc ggtcatcgtc gcgacgatat
    13381 cgatcgggtc gttgggctgg aacttggcgc tgacgactat ctcacaaagc cgttcaatct
    13441 gcgcgaactg ctcgcccgcg tccgcgcggt gctgcgccgg ttcgacagcg ggcgtgcgcc
    13501 ggtgcgcgag ccggagcgcg gccgctggcg tttctctggc tggcaactcg accgcagaac
    13561 gagacggctc accgatcccg acggcgctcc ggtggtgctg accaagggcg agtacgcgct
    13621 gcttgttgcc ttccttgagg cgccacagcg tccactcagt cgcgagcacc tgctccaggc
    13681 gacgcgggtc cacgaggatg tgttcgaccg cagcattgac gtgcagatcc ttcggttgcg
    13741 tcgcaagttg gagcgcgacc cgagcgcccc acgggtcatc caaaccgagc gcggtgtcgg
    13801 gtatgtattt gccgttccgg tggaacgggt ttgatggggc ttacttttcg tgcggcagcc
    13861 gatatgcacg gctcgatccg attgatgctc aatcagcccg aacaagataa gagatgaatt
    13921 ataagagtct gaaatcttcg aaaggatcga tcgaaaggtt cgggggtaac cgccacaagt
    13981 aggcaatgtc tcgatctttc gtttcgggca ggaagatcaa accgacgacg aaactcatcg
    14041 ccgccacgac gattggatac caaagtcctg aatagatgtc cccggttgcg gctacaatgg
    14101 cgaacgccgt ggcgggcagg aacccgccga accagccatt gccaatgtgg tacggcagcg
    14161 acaccccgct gtatcggatg cgggttggga acagttccac cagcaaagcg gcgattggtc
    14221 cgtagaccat ggtgacgtag atcacaagga tcgtgagaag gataaccgtc atgaccgcgt
    14281 ttatccgcga cgggtcggcc gttgcaggat aaccatgcgc ggtgatggca tcattcagca
    14341 ctcgggcgaa atctggcgcg gtagtcggaa ctatctgttt gcccacccgg acgctcgccg
    14401 gcgtcatcgg cggcgccgct tcgttccgat agttcactga gcggtcgacc agggcagctt
    14461 tggcgatgtc acagggtgag gtgaacgctg ccgtgccaac cggattgaac tggaagtggc
    14521 actctgcagg atcagcgatc acaacgactg gtgcctcggc gagggctgtc tcgagggcgg
    14581 ggttcgcaaa atgcgtgatg ccttgaaata gcgggaaata ggtcacgact gcgagaaaga
    14641 agccgagcaa catgatgggc tggcgaccga tcttgtcgga gagccaaccg aacaggatga
    14701 agaagggagt acctatcgca agcgcgatcg ccaccaagat ctgcgcggtc gcgcctggca
    14761 ccttcaaggt ttcggtcaga aagagcagcg catagaactg tccgccatac cagatcacgg
    14821 cttcacccgc cgttgcgccc aacagagcaa ggattgccat cttggcgttt tgccagcggc
    14881 cgaatgcttc agtgagcggt gcttctgaac cctcgccttg ttccttcatg cgctggaaga
    14941 tcggcgattc ttgaagctta aggcgaatcc atatcgaaac ggcaagcagc atgattgaaa
    15001 acaagaacgg caggcgccaa ccccactcgc cgaacgcctc ttcgcccatc cacgtgcgga
    15061 taccaaggat gatcaagagc gccatgaaga ggccaagcgt cgccgtggtc tgaatccaag
    15121 acgtgtaata cccgcgttta ccaggcggag cgtgttcagc aacgaagatt gctgctcctc
    15181 catattcgcc gccgagcgcc aaaccctgga cgagacgcaa gccgatcaga atgatcggcg
    15241 cgataatgcc ggccgtcgcg taggagggca ggacgccgat caggaacgtg ctgagaccca
    15301 tcagggtcat ggtgatcagg aaggtatact tgcggccgac gagatcaccc gtccgtccga
    15361 acagcaaggc gccgaacgga cgcaccgcaa agcccgccgc aaatgcgagc agagtgaaga
    15421 tgaagccgac ggcgggggca acattggaaa agaaatattt ggcaagaaac acccctaagg
    15481 tgccgtagat gtagaagtcg taccattcga agacggtacc gagcgaggag gcgaggataa
    15541 cgagccgttc ctcggcggtc atgccgctct cttctcgccg gggcagaccg gctgcgatgg
    15601 tagacatagc cttcggccca aaagcttgat tgtccgtggg acggcgcggg cggaaccaag
    15661 ccagcgaccc gacccagatt gtgggcacgt tcgcactttt gctgtcagcg gccaagcgac
    15721 tcggtaagaa accggcgttc gtcggatttg cgaaaggctt tatcctacgc gccagcctga
    15781 aaagtacgaa ttataccgat ccacccgcat ctcatcttta catgagggtt cgaatgtcaa
    15841 atggtctgcg gcatcgtttt ctccgattgt gagtggtgac atatcgatac gtgattgggt
    15901 ccagggctgc ctgtctcgca gctacggctg cgtcaacctt acatgcggtc gggcc
//
LOCUS       DATLJT010000177         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2306217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000177 DATLJT010000000
VERSION     DATLJT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2306217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VM910_04010"
     CDS             complement(<1..936)
                     /locus_tag="VM910_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HVI61749.1"
                     /translation="MSGMLPQFSDIVWPEAAAYWIANARAPLCMLGEARPGAQYDCDD
                     GIAPVDLLIESERLARMVPAGTAGTDQHPRIDLRGRQVWPMLVDVHTHLDRAHTVVRA
                     PNVSGSFADALRATASDRLRWTRDDLLMRMNFGLRCAYAHGVSAIRTHLDSVPAQAER
                     SWGAFREVRAEWAGKVALQAVSLVPIDLFRDEGGDQLAAVVAKSGGVLGAVTRAANGT
                     HGALLDDLDALLDRIFSLAARHDLDLDFHVDETNDPRAAALPHVARAVLRHDYQGRVV
                     AGHCCSLAVQPEEQADRTLDLLAEAEVAVVTLPTVN"
     gene            complement(941..1108)
                     /locus_tag="VM910_04015"
     CDS             complement(941..1108)
                     /locus_tag="VM910_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HVI61750.1"
                     /translation="MPGQAFLRAELTTNNFKSLDPERAIAVLPGTAIEQHDPQLPVMT
                     DAAMLEDMIGR"
     gene            complement(1215..2129)
                     /locus_tag="VM910_04020"
     CDS             complement(1215..2129)
                     /locus_tag="VM910_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI61751.1"
                     /translation="MLHSRLLKYLDEVARLGSIRKAALRLNVASSAINRQILALENEL
                     GAPIFERMPRRLRLTATGEVLIAHVRETLKNHQRVETHIEALKGLTRGEATIATMNGL
                     AAGPLPRFLNSILEMHPRVRLRLKILPFDQISNAVLTGEADLGLSYGSSPSPGLRVVA
                     SHDLSLGAVVSPKHPLTTRRPLRLADCAEFPLAIADASMTIRPAVELAFTRANLPLNA
                     TIETNSIEFLKKVARNGHAITFLNPVDVDDEVRAGEIRHLVLPEFIQHPISLKLIVRA
                     RGGLEAFPSLVVEELRKAMPKLEPIGGA"
     gene            2385..3371
                     /locus_tag="VM910_04025"
     CDS             2385..3371
                     /locus_tag="VM910_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61752.1"
                     /translation="MPLRTTTTGIAAALALGIALPPAKAADKVTFLTSWYAQAEHGGF
                     YQAKAAGLYEKANLDVTIKMGGPQVNGLQLLLAGEADIMMGYDFQVLTSLSKGIPVVT
                     IAASFQKDLQGLMTHDHVKGLDDLKDKTILVATPGRSSWWPWLRRKFGYSDAQTQPYT
                     FNLQPFFNDKNVVQQSYPSSEPFQALQKGVPVKFFLFADNGYPPYGTTMVTTTKLVAE
                     RPDVAQRFVRATIEGWKSYLKGNAAPANALIKADNPKMSDEQIAFGIKRLNELEVADG
                     GDAKTMGIGIITEARWKASYELMVNEGLLPKETDWTKGFTTQFVKDLKITAR"
BASE COUNT          652 a         1073 c         1069 g          589 t
ORIGIN      
        1 attgaccgtc ggcagcgtca ccaccgcgac ctcggcctcg gccaggagat cgagcgtccg
       61 gtcggcctgc tcctcgggct gcaccgcaag gctgcagcaa tggccggcga ccacgcgccc
      121 ctggtagtcg tggcgcagga cggcgcgggc gacatgtggc agcgccgcgg cgcgcgggtc
      181 gttggtctcg tcgacgtgaa agtcgaggtc gagatcgtga cgcgcggcga ggctgaagat
      241 tcggtcgagc agcgcgtcga gatcgtcgag cagagcaccg tgcgtgccgt tggccgcgcg
      301 cgtcaccgcg ccgaggacgc cgcccgattt cgctaccacc gcggcgagct gatcgccgcc
      361 ttcatcgcgg aacagatcga ttggaacgag cgatactgcc tgcagcgcga ctttgccggc
      421 ccattccgcg cgcacctcgc ggaacgcgcc ccaacttcgc tcggcttgcg ccggcaccga
      481 gtcgagatgg gtgcggatgg cagatacgcc atgggcatac gcgcaacgca ggccaaagtt
      541 catgcgcatg agcaggtcgt cgcgtgtcca gcgcagccga tcggacgcgg tcgcgcgcag
      601 cgcgtcggcg aatgaaccgg agacgttggg cgctcggacc acggtatgag cgcgatcgag
      661 gtgggtatgc acgtccacca gcatcggcca cacctggcgg ccgcgcagat caatgcgtgg
      721 atgttggtcg gtccctgccg ttccggcggg caccatgcgc gcgagccgct cgctctcgat
      781 caacagatca accggcgcaa tgccgtcgtc gcaatcgtac tgggcgccgg gcctcgcctc
      841 ccccagcatg caaagcggcg cgcgcgcatt ggcgatccaa taggcggcag cctcgggcca
      901 gacgatgtcg gagaactggg gcagcatccc ggacatggtt tcaccgccca atcatgtctt
      961 ccaacatggc ggcgtcggtc ataaccggca gttgcggatc gtgctgttcg atcgcagtcc
     1021 ccggcaggac ggcaatcgcc cgttctgggt cgagcgactt gaagttgttg gttgttaact
     1081 cggcccggag gaacgcctgt cctggcacgt caaatctccg tcgccgcttg cggtgccaaa
     1141 gccccacgcc aagcggacat cataggaccg acttggcttc ggcaagccct tatttgcacc
     1201 cgttgtgctg ccaattaggc accgccgatc ggctccagtt tgggcatggc cttgcgcaac
     1261 tcctcgacca ccaggctggg aaaagcctcg agtcccccgc gggcgcggac aatgagctta
     1321 agcgaaatgg gatgttggat gaattcaggc aataccaggt ggcggatttc gcccgcgcgc
     1381 acctcgtcgt cgacgtcgac cgggttgagg aaggtgattg catggccatt gcgcgcgacc
     1441 tttttcagga attcgatcga gttggtttcg atcgtcgcat taagcggaag attggcacgg
     1501 gtgaaggcca gttccaccgc aggacggatc gtcatcgagg cgtcggcaat cgccagtggg
     1561 aactcggcgc aatccgccag cctcagcggg cgccgcgtcg tcaatgggtg tttcggcgag
     1621 accacggcgc cgaggctgag gtcgtggctc gccacgaccc gcaatcccgg actcggcgac
     1681 gagccgtagg acagccccaa atcggcttcg ccggtcaaaa ctgcgttgga aatttggtcg
     1741 aacggaagaa ttttgagccg caaacgaacg cgcggatgca tttccagaat gctgttgaga
     1801 aagcgcggca gcggtccggc ggcaagccca ttcatggtgg cgatggtggc ctcgccgcgc
     1861 gtaagcccct tcagcgcctc aatatgggtt tcgacccgct gatgattctt cagcgtttcg
     1921 cgcacgtgtg cgatcagcac ctcgccggtc gcggtcaggc gcaagcgccg cggcatgcgt
     1981 tcgaagatcg gggcgccgag ctcgttttcc agcgccagga tctgccgatt gatcgcggag
     2041 gaagccacgt taagccgtaa cgctgccttg cgaatggagc ccagccgggc gacctcgtcg
     2101 agatatttca acagccgcga atgcaacatg gcggcgatcc tcgctacggg cggatcgagt
     2161 gagcaacatc gagctatggg cgcgggcgcg acttgcctcg ctcctcatcg cctgtgttca
     2221 agaccccatc actgatgctt caaaggcatc gatgatagcg aaatttgatg ctttttagaa
     2281 ccattcgcct gtgcgcagat tgagtttagg cgcactctcc cgagtccctg acggaggact
     2341 gcggccggtc gcggccgctc ttccgcaacc acagcaggtc aaccatgcca ctccgcacca
     2401 ccacgacagg gatcgccgca gcattggcgc tcggcatcgc cctgcccccc gcgaaagctg
     2461 ccgacaaagt aaccttcctg accagttggt atgcgcaggc cgagcacggt ggcttttacc
     2521 aagccaaggc ggccggcctc tacgagaagg ccaacctcga cgtcacgatc aagatgggcg
     2581 gcccccaggt caacggcctg cagctcctgc tcgcaggcga ggccgacatc atgatgggct
     2641 acgatttcca ggtgctcacg tcgctgtcga aaggcattcc ggtcgtcacc atcgccgcgt
     2701 ccttccagaa ggatctgcag gggctgatga cgcatgatca cgtcaaaggc ctcgatgacc
     2761 tcaaggataa gaccatcctg gtggcgacgc ccggccgctc cagctggtgg ccctggctgc
     2821 ggcgcaagtt tggctacagc gatgcgcaga cccagcccta cacgttcaac ctgcagccgt
     2881 tcttcaatga caagaacgtg gtgcagcaat cctatccatc atcggagccg ttccaagcgc
     2941 tgcagaaggg cgtgcccgtc aagttcttcc tgtttgccga caacggctat ccgccctacg
     3001 ggaccaccat ggtcacgaca accaaactgg tggcggagcg gccggacgtg gcgcagcgct
     3061 tcgtccgggc cacgatcgaa ggctggaaaa gctatctcaa gggcaatgcc gcgccggcca
     3121 acgccctcat caaggccgac aatccgaaga tgagcgacga gcagatcgcg tttgggatca
     3181 agcgcctcaa tgagcttgag gttgccgacg gcggcgatgc caagaccatg ggcattggca
     3241 tcatcaccga ggcgcgctgg aaggcctcct acgagctgat ggtgaacgag ggcctgctgc
     3301 ccaaggaaac cgactggacc aagggcttca cgacgcagtt cgtgaaggac ctcaagatca
     3361 ccgcccggtg atatgattga cgc
//
LOCUS       DATLJT010000178         1892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2765807,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000178 DATLJT010000000
VERSION     DATLJT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2765807"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="VM910_04030"
     tRNA            complement(1..76)
                     /locus_tag="VM910_04030"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Phe,seq:gaa)
     gene            complement(205..393)
                     /gene="yacG"
                     /locus_tag="VM910_04035"
     CDS             complement(205..393)
                     /gene="yacG"
                     /locus_tag="VM910_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HVI61753.1"
                     /translation="MPPEAEKKQGARKVRRCPICGKPAQEATRPFCSPRCRDVDLNRW
                     LSGSYVVPGSENDEEDEK"
     gene            complement(377..1006)
                     /locus_tag="VM910_04040"
     CDS             complement(377..1006)
                     /locus_tag="VM910_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HVI61754.1"
                     /translation="MLGRPKFVLASGSPRRLALLNQAGIEPDALRPADVDETPRRGEL
                     PRACANRLARAKADAALKSVQLDDELRGSFILAADTVVAVGRRILPKAHLVDEAAQCL
                     RLLSGRNHRVYTAICLVTPKEAFRQRLIETRVRFKRLSEDDIQAYIGSGEWRGKAGGY
                     AVQGIAGSFVVKMVGSYSNVVGLPLYESMTLLGGEGFPIRFGWLNAAGG"
     gene            complement(1266..1730)
                     /locus_tag="VM910_04045"
     CDS             complement(1266..1730)
                     /locus_tag="VM910_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HVI61755.1"
                     /translation="MAAPRVRNPQSVLFACGLNSVRSPMAASILHQMFPQALYVRSAG
                     VKKGELDPFAVAVMAELGQDISTHKPTTFEELEDWEGLNFDLIVTLSPEAHHKALDLT
                     RTLAADVEYWPTFDPTGTEGNREQRLAAYRELCDTLLARIRRRFAKVSAASG"
     gene            complement(1737..>1892)
                     /locus_tag="VM910_04050"
     CDS             complement(1737..>1892)
                     /locus_tag="VM910_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HVI61756.1"
                     /translation="PDKIEAIDMGRRGIHDEGSRTLQERLKGKVRVDFETARRLFTLI
                     TVLHWKG"
BASE COUNT          343 a          594 c          604 g          351 t
ORIGIN      
        1 tggtgcccag gggcggaatc gaaccaccga cactgcgatt ttcagtcgca tgctctacca
       61 actgagctac ctgggcaggt cgcggaagtc cgaaaaggac cggcgagccg gcgccttata
      121 gtggggtcgg acggccctgt ccacctcgct tcggctacgg tggcgcatgc ctgacccaaa
      181 tcccaaaata ttgggaaatc aggcctattt ctcgtcctct tcgtcgttct cggagcccgg
      241 caccacatag gagccggaca gccagcggtt caaatcgaca tcgcgacagc gcggcgagca
      301 gaacgggcgt gtcgcctcct gggccggctt gccgcagatc gggcaccgcc tcaccttgcg
      361 cgcgccttgc ttcttttcag cctccggcgg cattgagcca gccgaagcgg atcggaaagc
      421 cctcgccgcc gagcagcgtc atggactcat agagcggcag tccgaccaca ttggaataag
      481 agccgaccat cttgaccacg aaggagcctg caatcccctg cacagcatag ccgccggcct
      541 tgccgcgcca ttcgcccgag ccgatgtagg cctggatatc gtcctcgctc aaccgcttga
      601 aacgcacgcg cgtctcgatc agccgctggc gaaaagcttc cttcggcgtc accagacaga
      661 tcgcggtgta aacgcgatgg ttgcggcccg agagcagccg aagacattgc gcggcctcat
      721 cgaccagatg ggccttgggc aaaatgcggc ggccgacggc gaccacggtg tcggcggcaa
      781 ggatgaagga gccacgcagt tcgtcatcga gctgcaccga tttcagcgcg gcatccgcct
      841 ttgcccgcgc cagccggttg gcgcaggcgc gcggcagttc gccccgtcgc ggggtctcgt
      901 cgacgtcggc cggacgcagc gcatcgggct cgatgccggc ctggttgagc agcgccagac
      961 gccgcggtga tccggaagca agaacgaatt tggggcgacc tagcatgcgg aatcttgaac
     1021 gagggaatcg ggggcgaagc gagcgcgcgg aacgtatcgg atggcatccc gttccacaac
     1081 ccgttgcggc tgcttttgag gtggcgatct tacgggattg cgtcagtttg gatgcgcgat
     1141 ggcttcatgt cgaatcgcat cgcgctccag tttattgttt gaacatgatc tctgcgcaaa
     1201 cgcgttgcgc gtttgtcacg aagtgcgggg caggcttttg cggatcatgc tccggtttgc
     1261 atgtcctacc cgctcgccgc actcaccttg gcaaagcgcc tgcggatgcg ggcgagcagc
     1321 gtgtcgcaga gctcgcgata ggcggcaagc ctctgttcgc ggttaccctc ggtgccggtc
     1381 ggatcgaacg tcggccaata ttccacgtcg gcggcgagcg tgcgcgtgag gtcgagcgcc
     1441 ttgtgatgcg cctccggcga cagcgtgacg atcaggtcga aattgagccc ttcccaatcc
     1501 tcgagctcct cgaacgtggt cggcttgtgg gtggagatgt cctggccgag ttcggccatc
     1561 acggctaccg cgaacggatc gagctcgccc ttcttcacgc cggccgagcg cacatagagc
     1621 gcttgcggaa acatctgatg caggatgctt gccgccatcg gcgagcgcac gctgttgaga
     1681 ccgcaggcga acaacaccga ttgcggattg cgcacgcgcg gtgcagccat tggaggctag
     1741 cccttccaat gcaggacggt gatcagcgtg aacaggcggc gggcggtttc gaaatcgacc
     1801 cgcaccttgc ctttcaacct ttcctgaagc gtgcgcgagc cttcgtcatg aatgccgcgg
     1861 cggcccatgt cgatggcctc gatcttgtcg gg
//
LOCUS       DATLJT010000179         1799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_468091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000179 DATLJT010000000
VERSION     DATLJT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /gene="hrcA"
                     /locus_tag="VM910_04055"
     CDS             complement(<1..443)
                     /gene="hrcA"
                     /locus_tag="VM910_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323502.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVI61757.1"
                     /translation="MTSHELPLGANQLGLAQLNERSREIFRLIVESYLATGEPVGSRN
                     LSRILPMTLSPASVRNVMSDLEQLGLVYAPHTSAGRLPTEMGLRFFVDALMQIGDLNE
                     ADRRAIEAQVAASGQAKSVEAVLTEASGLLSGLSRAAGVVLTAKS"
     gene            630..1343
                     /gene="rph"
                     /locus_tag="VM910_04060"
     CDS             630..1343
                     /gene="rph"
                     /locus_tag="VM910_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HVI61758.1"
                     /translation="MRPSRRQPDELRPVSLERGVVKYAEGSCFVKFGDTHVLVTATLE
                     DRLPPWLKGQARGWITAEYGMLPRATLERTRREASAGKQNGRTVEIQRLIGRSLRSVI
                     DLKALGERQITIDCDVIQADGGTRTASITGAWIALYDCLLWMKTRQMVEMDVLRDHVA
                     AISCGVCSGVAVLDLDYAEDSQADTDANFVLTGSGNIVEVQATAEKTPFSETQFSALL
                     ALARKGVAKLVDLQKMAVI"
     gene            1428..>1799
                     /locus_tag="VM910_04065"
     CDS             1428..>1799
                     /locus_tag="VM910_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HVI61759.1"
                     /translation="MRPLVSKDLVIATHNPGKLAEMRELLAACGIAAISIGELALPEP
                     AETGMTFVENACIKAAAAAKAAQLPALADDSGLVVEALDGAPGIHSARWAGPDRNFHR
                     AMEKIEQQLRERDATVPERRRA"
BASE COUNT          376 a          514 c          551 g          358 t
ORIGIN      
        1 ttcgacttgg cagtgagcac cacgccggcg gcgcggctta atcctgacag cagccccgaa
       61 gcttcggtca aaaccgcctc gactgatttg gcttggccgg aggccgcgac ttgcgcttcg
      121 attgctctgc gatcggcttc attgaggtcg ccaatctgca ttagggcgtc gacaaagaaa
      181 cgcagtccca tctctgtcgg caaacgtccc gccgaggtgt gaggagcgta aaccaagccg
      241 agctgttcga gatcggacat gacattgcgc accgaagccg gcgacaaggt cattggcagg
      301 atgcgcgaca gattgcgcga gccaaccggc tcgccggtgg caaggtagct ctcgacaatt
      361 aggcgaaaaa tctcgcgtga acgttcgttg agctgtgcga gacccagctg gttcgcgcca
      421 agcggcagtt cgtggctggt caaaacttca ctccgcagtc ttagacgacg tgctcgatga
      481 aatgatatgt atcattctga cgccgccatg gcacccagcg tgccttgtgc ggttaatccc
      541 tgcacccaat gcaaggtcaa actgggtcac ggttcatagt ttacaacatg cggccccggc
      601 gcgatgccgg caaacgttcg gagacatgca tgcggccgag ccgccgacag cccgatgaat
      661 tacgccctgt gtcgctcgaa cgcggggttg tgaaatatgc ggaaggttca tgcttcgtaa
      721 aattcggcga cacgcatgtg ctggtaaccg ccacgcttga ggatcggctg ccgccatggc
      781 tcaagggcca agcgcgcggc tggatcacgg ctgaatatgg catgctgccg cgcgcgacgc
      841 ttgaacgcac acgccgcgag gcttcggccg gcaagcagaa cggccgtacg gtagagatcc
      901 agcgcttgat cgggcggagt ctgcgctcgg taatcgatct caaggcgctc ggcgagcgcc
      961 agatcaccat cgattgcgac gtcatccagg ccgatggcgg aacgcgcacc gcttccatca
     1021 ccggcgcatg gattgcgctt tatgattgcc tcctctggat gaagacccgc cagatggtcg
     1081 aaatggatgt cttgcgcgat catgttgccg cgatctcttg cggtgtctgc tccggcgtgg
     1141 cggtgcttga tctcgactat gccgaggatt cgcaggccga taccgatgct aatttcgtgc
     1201 tcactggctc gggcaatatc gtcgaggtcc aagcaaccgc ggagaagaca ccgtttagcg
     1261 aaactcagtt ctcggctttg ctggcgctgg cgcgcaaagg cgtggccaag ctcgtcgatt
     1321 tacagaagat ggcggtgatc tgagtcggac gacaaaggtc agatgtcgga ggacggatgg
     1381 ttaatgcaaa agcgccgaaa ggttcagata gcgatttgat atctgacatc cgccctctgg
     1441 tttccaaaga cctggtgatc gcgacccaca atccaggcaa acttgccgaa atgcgcgaac
     1501 tgcttgcggc ctgtggaata gcggccatat cgattggcga gcttgctttg cccgaaccag
     1561 ccgaaactgg aatgaccttt gttgaaaacg cctgtatcaa ggccgcggcg gctgctaagg
     1621 cagcgcagct cccggccctc gccgatgatt caggccttgt tgtcgaggcg ctcgatggcg
     1681 cgcccggcat tcattcggcg cgttgggccg gtcccgatcg caattttcac cgtgccatgg
     1741 aaaaaatcga acagcaattg cgggagcggg atgcaactgt gccagaacgg cgccgggcg
//
LOCUS       DATLJT010000180         7516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_927518,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000180 DATLJT010000000
VERSION     DATLJT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927518"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VM910_04070"
     CDS             <1..723
                     /locus_tag="VM910_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61760.1"
                     /translation="YTLLMAENALGISQALYKKTSSSFDPVKQYDAIAVVATSPLVLA
                     VANNVKANSVNELIAYSRAMPQKINYASAGIGSVSHLTFEVIKAATGMDAVHVPYKGG
                     GPAMNDVIAGHVALNMASIQVAKGLIEAGKIKGLAVTSAERSPMLPNVPTLKEAGVKT
                     ADVDLRFWFGLFAPKGIPDAVKAKLDKAVSTTLSNPRVRERLANLDIEPAYAPGDALK
                     TKLENEIANWTKFIDAHGIKPE"
     gene            799..2595
                     /locus_tag="VM910_04075"
     CDS             799..2595
                     /locus_tag="VM910_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI61761.1"
                     /translation="MSKNMLDGGQVIVDYLVREKVPYVFGLCGHGNIGFLDALYERSD
                     DIKTVSVHHESVAGFMADVYYRVGGKPTATFTSCGPGSANLPIALANSYLDSVPFLAV
                     TGNVPTSQFNRGAFQELYRHYQADFPSTVRAYCKRVFQPTRGDMVPLAIRQAWKTMVT
                     GRPGPVVLDVPFDIFKEAAAEETPKPEEWNANISCRCGADPDGVTKAVDMLLSAKQPV
                     ILVGQGVKYGGAAADLLRLAERLRIPVAWSASGTGAIDSRHPLALGLIARNGPYQANH
                     AARTADVLLALGVRFDDRTSSSWLPGYSFTIPPTRLIHVDIDPDEIARNYPVALGLMA
                     DVRTFLHQVLAELESRDGLADAAAARAKWLDTIDGYRREWNAFIAPGFVEDASPIHPQ
                     RAAHEIDKVLPEDAILVSDIGVHHNWLIQFCNPRRPDSLIGSMGFGPMGFGVAGVLGA
                     QFAAPERPCVSVCGDGAFFMHASVLGTAVEYDLPVIWVVWNNFAYASIRGLQRGYLGG
                     RELATDFRHPDTGAPYNPDFAAMARSAGIAGVNVDRAADLADALRAALASRRPYLINA
                     NIGADKNPGGAGVWELPGLGQSQPAFGGRYQP"
     gene            2636..3607
                     /locus_tag="VM910_04080"
     CDS             2636..3607
                     /locus_tag="VM910_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61762.1"
                     /translation="MRRIIAAWIAAIFAALVASSAPAQTSFPARTVKFIVPFPGGGIN
                     DVLARIIGDKLQAKWGQPVVVENKTGAGGNIGADLAYQSEPDGYTLLLSPPGPLAINQ
                     TLYKHLSYKPQEFVPITVVGSVPNVVIVRKELPVTSLKELIDYVRANPGKVTFGSQGN
                     GATPHLTAMMFRGMTDTQMVHVPYRGETLVLNDMIGGHVDVFFGNIAAGGPPFRDGRV
                     KILAIADTHRSRVLPEIPTTAEAGLPGLVSTGWFALAAPPKTSPALASDIAKAAVEVI
                     KTPDVQAKFRAASVGPIGNSPAEMAAFIRAEAQRWGEVIKKNNIVVD"
     gene            3760..4923
                     /locus_tag="VM910_04085"
     CDS             3760..4923
                     /locus_tag="VM910_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI61763.1"
                     /translation="MDVAATDIDYPRPARAWKPERCDRIALVLQGGGALGAYQAGVFQ
                     ALSEADIEPDWVTGVSIGAINCAIIAGNSKQWRLKRLKTFWERITERKIWSYTPDGDV
                     FRKARNAASSWMTLVQGQPGFFAPRHPTPWFSFAGAQSATSYYDSTPLRETLEELVDF
                     SLLNEKRMRFSVGVVNVLSGNFAYFDNQHETIGPEHVMASGALPPALPMVKIGTDHFW
                     DGGIVSNTPLQYILDQDDPLNTLIFQVDLFSARGVLPRDIQDVMARHKDIMYSSRTRY
                     NTDVYRRIHTWKTRLHAALQKISDDRLSEDERRLREELATLPEITILQLIYQQKAYEG
                     HAKDYEFSGTSMREHWQSGYEDTKRTLKKQEWLRMPLEGAGIVVHDVHRESDR"
     gene            5170..6774
                     /locus_tag="VM910_04090"
     CDS             5170..6774
                     /locus_tag="VM910_04090"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HVI61764.1"
                     /translation="MRFAALAASLVGSVVFAVLIPYNVVFIALFALCAILSAIGIWDL
                     LQSKHSVRRNYPILANIRFMLEKVRPEIRQYFLESETDGTPFNRSKRAVAYQRAKGAL
                     DKRPFGTQQDIYGNNFEWINHSIIPCPVEGHDFRITVGSADCRKPYALSLFNISAMSF
                     GALSANAIRALNRGAKLGGFAHDTGEGGISRYHREFGGDLIWEIGSGYFGCRNEDGSF
                     CADRFAAQAQERQVKMVEIKLSQGAKPGHGGVLPGAKVTAEIANARGVPIGVDCISPA
                     RHSAFKTPVEMMHFIAQLRGLSGGKPVGFKLCIGHAWEFMALCKATLETGIRPDFIVI
                     DGSEGGTGAAPLEFVDHVGTPLREGLVLAHNCLVGAGLRQSVRLGASGRVVTGFDIAR
                     LLALGADWCNAARGFMFALGCIQAQSCHTDRCPTGVSTQNPWRQRALVVDDNAARVRQ
                     FHQSTLAALGELVGAAGLAHPSHLRPMHILKRVSPSEVKNFAEVYTFLQHRELISGVG
                     HTHYSEQWALTDAQHFAPLPHVQSAA"
     gene            6881..7096
                     /locus_tag="VM910_04095"
     CDS             6881..7096
                     /locus_tag="VM910_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61765.1"
                     /translation="MSTKAHKGGSHHEIAAEHHETAAHHHREAAKHYESGDHEKAGHH
                     AHVAHAHGLHATHHGHEAAKHHAEHHK"
     gene            7378..7516
                     /locus_tag="VM910_04100"
                     /pseudo
     CDS             7378..7516
                     /locus_tag="VM910_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
BASE COUNT         1458 a         2412 c         2317 g         1329 t
ORIGIN      
        1 tacacgctgc tgatggcgga aaacgcgctc gggatcagtc aagccctcta caagaagacc
       61 tcgtcgtcct tcgatccggt caagcagtac gacgcgatcg cggtggtcgc gacgtcgccg
      121 ctggttctgg cggtcgccaa caacgtcaaa gccaattcgg tgaacgagct catcgcgtat
      181 tcacgcgcga tgccgcagaa aataaactat gcctccgccg ggattggcag cgtctcgcat
      241 ctaacctttg aagtgatcaa ggcggccacg ggaatggacg cggtccacgt tccctacaag
      301 ggcggcggcc ccgccatgaa cgacgtgatc gcgggccacg tggccctcaa catggcatcg
      361 atccaggtcg ccaagggcct catcgaagcc ggcaagatca aggggctcgc ggtcacgagc
      421 gccgagcgct cacccatgct gcccaacgtt ccgacgttga aagaagccgg cgtcaagacc
      481 gccgacgtgg atctgcgctt ctggttcggc ctcttcgctc ccaaaggcat tccggatgcc
      541 gtcaaggcga agctcgacaa ggccgtctcg accaccctgt cgaatccgcg cgtgcgcgaa
      601 cggctggcga atctcgatat cgaaccggcc tacgcgccag gcgatgccct gaagacgaag
      661 ctcgaaaacg agatcgcaaa ctggacgaag ttcatcgacg cccatggcat caagccggaa
      721 tgagccgcgc gggcgcgcta taccaagatc atcagggacg cgaatatcaa accggaataa
      781 cagcgaagac caatgccgat gtccaagaac atgctcgacg gcggtcaggt gatcgtcgat
      841 tatctcgtgc gcgagaaggt gccttacgtc tttggcctgt gcggccacgg caatatcggc
      901 ttcctcgacg cgctctacga acgctccgac gacatcaaga ccgtctccgt ccaccacgag
      961 agcgtcgccg gcttcatggc ggacgtgtac taccgcgtcg gcggcaagcc gacggcgacg
     1021 ttcacctcct gcggtccggg ttcggccaat ctgccgatcg cgctcgccaa ctcctatctc
     1081 gattccgttc cgttcctggc ggtgaccggc aacgtcccga ccagccaatt caaccgcggc
     1141 gcctttcagg aactctatcg ccactatcag gcggactttc cgtcgaccgt acgcgcctat
     1201 tgcaaacgcg tgtttcagcc cacgcgcggc gacatggttc cgctcgcaat ccgtcaggcg
     1261 tggaagacca tggtgacggg gcggccgggg ccggtcgtgc tcgatgtccc cttcgacatc
     1321 ttcaaggaag cggccgccga ggaaaccccc aaaccggaag agtggaacgc caacatctcc
     1381 tgccgctgcg gcgccgaccc tgacggcgtg acgaaggccg tcgacatgct gctctccgcc
     1441 aagcagccgg tcatcctggt cggccaaggc gtcaagtacg gcggcgcggc ggcggatttg
     1501 ttgcggctgg cggaacgctt gcgcattccg gtcgcgtggt cggcgagcgg caccggcgcc
     1561 atcgattccc gccatcccct ggcgctcggt ctcatcgccc gcaatggtcc ctatcaggcc
     1621 aaccatgccg cgcgcaccgc cgacgtgctg cttgcgctcg gcgtgcgctt cgacgatcgc
     1681 acctcgagct cgtggctgcc gggctattcg ttcacgatcc cgcccaccag gctcattcac
     1741 gtcgacatcg acccggacga gatcgcccgc aactacccgg tcgcgctcgg gctgatggcg
     1801 gacgtgcgca cctttctcca ccaagtgctg gcggagctcg agtcgcgcga cggccttgca
     1861 gacgcggccg ccgcgcgtgc gaaatggctt gacaccatcg acggctatcg ccgcgaatgg
     1921 aacgcgttca tcgcgccggg atttgtcgag gacgcttcgc cgatccatcc gcagcgcgcc
     1981 gcccacgaga tcgacaaagt gctgccggag gacgccattc tggtgagcga catcggcgtc
     2041 caccacaact ggctgatcca gttctgtaat ccgcgacggc cggattcgct catcggatcg
     2101 atgggtttcg gcccgatggg attcggcgtc gccggcgtct tgggcgcgca gttcgccgcg
     2161 cccgagcggc cttgcgtttc ggtgtgcggg gacggcgcct tcttcatgca tgcgagcgtg
     2221 ctgggcaccg cggtcgaata cgatctgccg gtcatctggg tagtgtggaa caacttcgcc
     2281 tatgcctcga tccgcggatt gcagcgcggc tatctcggcg gtcgcgagct tgcgaccgac
     2341 ttccgccatc ccgacaccgg cgcaccttat aatcccgact ttgccgcgat ggcgcgttcg
     2401 gccggcatcg cgggcgtcaa cgtcgaccgc gcggccgatc ttgccgacgc gttgcgagcc
     2461 gcgcttgcgt cacgccggcc ctatctcatc aatgccaata tcggtgccga caagaatccg
     2521 ggcggcgcgg gcgtgtggga gctccccggc ttgggccaga gccaaccagc cttcggcgga
     2581 cgctaccaac cctgatggcg gctgcgaggc gtcatcggag cgggagggaa cgggagtgag
     2641 aagaatcatc gcggcatgga tcgccgccat ctttgcggcg ctcgttgcga gctccgcccc
     2701 tgcccaaacc tcgttccccg cccgcaccgt gaagttcatc gtccccttcc ccggcggcgg
     2761 tatcaacgac gtgctggccc gcatcatcgg ggacaagctc caggccaagt gggggcagcc
     2821 ggtcgtggtc gagaacaaga ccggtgcggg cggcaatatc ggcgccgacc ttgcttacca
     2881 atccgagccc gacggctaca cgttgttgct cagcccgccg ggcccgctgg cgatcaacca
     2941 gacgctctac aagcacctct cctacaaacc gcaagaattc gtgccgatca cggtcgtcgg
     3001 ctcggtgccg aacgtcgtca tcgtgcgcaa ggagttgccg gtgacgtcgc tcaaggaatt
     3061 gatcgactat gtcagggcga accctgggaa agtgaccttc ggcagtcagg gcaacggcgc
     3121 cacgccccat ctcaccgcca tgatgttccg ggggatgacc gacacgcaga tggtgcacgt
     3181 gccctaccgc ggcgaaacgc tggtgctcaa cgacatgatc ggcggccacg tcgacgtctt
     3241 ctttgggaat atcgcggcgg gcggcccgcc cttccgcgat ggcagggtga agatcttggc
     3301 gattgccgat acccaccgct cgcgggtgct gcccgagatt ccaaccaccg ccgaggccgg
     3361 cttgccgggg ctcgtgtcga ccggctggtt cgcgctggca gcgccgccga agacatcgcc
     3421 ggcattggcc tccgacatcg ccaaagcagc ggtcgaagtg atcaagacgc cggacgtgca
     3481 ggccaagttc cgcgctgcaa gcgtcggccc catcggcaac agcccggccg agatggcagc
     3541 cttcatcagg gcggaagcgc aacgctgggg cgaggtcatc aaaaagaaca atatcgtcgt
     3601 ggattgagac gtcgcaaagc gcagccggat tcgcgcgctg aggtcaagaa attcgcgaca
     3661 atgtgacaaa cgaacgaaca gcgccggaca aagacatttc cgtgtcatga tggcgtcata
     3721 gggcttcttc catggtgcgg gcttgagcgg gagcgctcca tggatgtcgc ggccacggac
     3781 attgactacc cgcggccggc gcgcgcttgg aagcctgaaa ggtgcgatcg catcgctctc
     3841 gttctgcagg gcggaggagc gctgggggcc taccaggcgg gggtgtttca agccttaagc
     3901 gaggccgaca tcgagcccga ttgggtgacg ggcgtctcca tcggagccat caactgtgca
     3961 atcatcgccg gcaattcgaa gcaatggcgg ctcaagcggc tgaaaacgtt ttgggagcgc
     4021 attaccgaac gcaagatttg gagttacacg ccggacggcg acgtctttcg caaggcacgc
     4081 aacgcggcca gctcatggat gactctggtc caaggccagc cggggttctt cgcgccgcgc
     4141 catccaaccc cgtggttcag ctttgccggg gctcaaagcg ccacgagcta ttatgacagc
     4201 acgccgctgc gtgagacgct cgaagagctg gtcgatttct cgctactcaa cgaaaagcga
     4261 atgcgctttt cggtgggcgt ggtgaacgtg ttgtccggga acttcgcgta tttcgacaac
     4321 caacacgaaa cgatcgggcc ggagcacgtc atggcgagcg gcgcccttcc cccggcgctg
     4381 ccgatggtca agatcggcac tgatcatttc tgggacggag ggatcgtctc caataccccg
     4441 ctgcaataca tcctcgacca ggacgacccg ctcaacaccc tcatcttcca ggtcgacctt
     4501 ttcagcgcgc gcggcgtcct tccgcgcgac atccaggatg tcatggcgcg gcacaaggac
     4561 atcatgtatt cctcgcgaac gcgctacaac accgacgtct atcgacggat tcatacgtgg
     4621 aagacccgcc ttcacgccgc gctgcagaaa atctccgatg accggttgtc ggaggacgag
     4681 cgccggctgc gagaggagct cgcaaccctc cctgagatca cgatcctgca attgatctac
     4741 cagcagaaag catatgaggg gcatgccaag gattatgaat tttcgggcac ttccatgcgc
     4801 gagcattggc aaagcggcta cgaggatacc aagcgcacgc tcaagaagca ggagtggcta
     4861 agaatgccgc ttgaaggcgc gggcattgtc gtgcacgacg tccatcgcga gagcgaccga
     4921 tagggacgtg gcggagcttt ggcgctttcc caaggggaga ctggtcgcgc ctcaaaaaat
     4981 gcttcgatag atcaaccttc ctaaccgagc cgcgatcctg gcgcgttgac gggccggacg
     5041 gcgctgacac ggaactgtca tctagcgaac ttaccattcc ggcgccatgc gcccgatcga
     5101 cggcgggcgc agatccttcc caaagattgc aacagcaacc gcgaaagcaa cggcggtgtg
     5161 gggagtgcca tgagatttgc agcactagcg gcaagcttgg ttggaagcgt cgtttttgca
     5221 gtgcttattc cctataacgt cgtgtttatc gcgctgtttg cgctttgcgc aatattgagc
     5281 gcaatcggca tctgggatct tttgcaatcc aagcacagcg tgcggcgcaa ttaccccatc
     5341 ctcgccaaca tccgcttcat gctggaaaag gtcagaccgg aaattcgtca atattttctg
     5401 gagagcgaaa ccgacggcac accgttcaat cgatcgaaac gggccgttgc ttaccagcgg
     5461 gcgaagggcg cgctcgacaa gcggccgttc ggcacccagc aggacatcta cggcaacaac
     5521 ttcgagtgga tcaatcattc gatcattcct tgtccggtcg aaggtcacga ctttcgtatc
     5581 acggtgggca gcgccgactg ccgcaagccc tacgccctgt cgctcttcaa catttcggcc
     5641 atgagcttcg gggcgctgag cgcaaacgcc attcgcgctc tcaaccgggg agccaagctc
     5701 ggaggcttcg cgcacgatac cggcgagggt ggcatcagcc gctatcatcg cgaattcggc
     5761 ggcgacctga tctgggagat cggcagcggc tattttggct gccgcaatga agacggctcg
     5821 ttctgcgccg accggttcgc cgcgcaggcg caagagcggc aggtcaagat ggtcgagatc
     5881 aagctgtcgc aaggggccaa gcccggccac ggcggcgtgt tgcccggcgc caaggtgacc
     5941 gcggagattg cgaacgcgcg aggtgtgccc atcggcgtcg attgcatctc gccggcgcgc
     6001 cattcggcgt tcaagacccc ggtcgagatg atgcacttca tcgcgcaatt gcgcggcttg
     6061 tccggcggca agccggtcgg cttcaagctc tgcatcggcc acgcctggga attcatggcg
     6121 ctctgcaagg ccacgctgga aactggaatt cgtccggatt tcatcgttat cgacgggtcg
     6181 gagggcggca ccggcgcggc accgctcgaa ttcgtcgatc acgtcggcac gccgctgcga
     6241 gaaggtttgg tgctcgccca caactgcctg gtgggggcgg gactgcggca atccgttcgc
     6301 ctgggcgcga gcgggcgggt ggtgaccggt ttcgatatcg cgcggttgct ggcgctcgga
     6361 gccgattggt gcaatgcagc ccgcggcttc atgttcgcgt tgggctgcat ccaggcgcaa
     6421 tcctgtcata ccgaccgttg cccaaccggc gtctcgacgc agaatccttg gcgccaaagg
     6481 gccctcgtgg tcgacgacaa tgccgctcgc gtgcgccaat ttcaccaaag cacgctcgcc
     6541 gccttgggcg aactggtggg tgcggctgga ttggctcacc cgagccatct tcgtcccatg
     6601 cacatcctca aacgagtttc gccgagcgaa gtcaaaaact tcgcggaggt ttatacgttc
     6661 ctgcaacacc gcgagctcat ctcgggtgtc ggtcataccc actattcgga gcaatgggcg
     6721 ctgacagacg cacagcattt cgcaccgctg ccgcacgtgc aatctgctgc gtagctgctg
     6781 aagccgctaa tccaccgatg cgaccgtcac gttcatacgc ctgtcagatt ccgatcgcag
     6841 tctgaccgcc gtcaatggac cagagcgaga ggagagcaac atgagcacaa aggcccacaa
     6901 gggtggcagc caccacgaga tcgcagccga gcatcatgag actgccgcgc accatcatcg
     6961 tgaggcggcg aagcactacg aatcgggcga tcacgaaaag gcgggccatc acgcgcacgt
     7021 cgcgcatgcg catggactgc atgcgaccca tcacgggcac gaggccgcaa aacaccacgc
     7081 cgagcaccat aagtaagcgc ggcgagcggg cagcccgccc ctccggccga acgataccgg
     7141 tcggctcgga acccatcaac gagctttacg tcgatgaagc tttggtgatg gcgcttgatc
     7201 gggcgcgcgc cgccgcgcgg ccggctcatc gaccctgcgt caagacgatg cgtcacatgc
     7261 ctgtcacaca gggcggcttt cctttcccgt aaccgctagc tccgaggcaa ccatgaccga
     7321 cattgccgca acgctgcacg ggcccgacca tgcggtggca gcggagaaac cgaagcggtt
     7381 tcgaccacgc acgtgttgtc atcgggtgtg gccggaacga tggcggccaa cggctcgggg
     7441 ctgcagtggg ctacggtgcg caacctgatc atggcgtggg tcctgacgct gcctgcagcc
     7501 atcacgcttt ccgcga
//
LOCUS       DATLJT010000181         3866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1846605,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000181 DATLJT010000000
VERSION     DATLJT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846605"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1705
                     /locus_tag="VM910_04105"
     CDS             341..1705
                     /locus_tag="VM910_04105"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI61766.1"
                     /translation="MTRQDYARAFELARSRAQGGDREAQFTLGWLFEKGLDDRFHESC
                     HKGGIHLCLEAPIYGSVGSVALGRGVEGHGSVAAGDRVVARRCGAAKLRSITHSRTEP
                     ASRVERARILLAYREDPSFFAVGRRLGLHHQTVQRCVERAVAEGPMAALDDRPRPGKE
                     PMITLEARTWLVSLACRKAKELGYPHELWTTRLLASHAREHGPAEGHACLARLAQGTV
                     CKILDQEDVKPHKVRYYLERRDPDFAEKMAEVLCVYRKVKLLKKAAAASRKKPSDAVA
                     VISYDEKPGIQAIATTAPDLPPVPGVHATFAREHEYKRHGTVTLMAGINLLTGKVHAL
                     VKDRHRSREFIEFLKLLDTAYPPHTAIHLILDNHSAHISKETRAWLADQPAGRFKFTF
                     TPKHGSWLNLVEGFFSKLARSVLRHIRVSSKQELKDRLMAAMDFFNRDPVVHTWTYKL
                     DKAA"
     gene            1835..2983
                     /locus_tag="VM910_04110"
     CDS             1835..2983
                     /locus_tag="VM910_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI61767.1"
                     /translation="MTAWRRVIELSIGDADVAKLTSIARSRTEPASRVERARILLGYR
                     EDPSFFAVARALGLHHQTVQRCIERALAYGPIAALDDRPRPGKEPKITAEAKAWLVSL
                     ACRKAKDLGYPHELWTTRLLAHHAREHGPAEGHACLDKLVQGTVCKILDAQEVKPHKV
                     RYYLECRDPEFKEKMAEVLCVYRQVKILKETAAVSKKKPADAVAIISYDEKPGIQAIA
                     TTAPDLPPEPGVHATFARDHEYKRLGTVSLLAGIDLLTGQVHALAKDRHRSREFIEFL
                     RLLDTAYPARTAIKLILDNHSAHISKETKAWLAQQPAGRFEFTFTPKHGSWLNLVEGF
                     FSKLARSVLRHIRVASKQELKDRIMAAMDDINQHPVVHTWSYKLDKAA"
     gene            3195..3572
                     /locus_tag="VM910_04115"
     CDS             3195..3572
                     /locus_tag="VM910_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61768.1"
                     /translation="MTHGSFVWNELYTRDVEAAKAFYAATIGWTFEGMPMPHQNRTYW
                     IARTGGKPVAGILDMRGIVPDSEPPHWLSYLEVDDVDRRLAEIERHGGRIVRPPFDVP
                     NFGRIAIGADATGAFLAWVTSKR"
BASE COUNT          757 a         1200 c         1202 g          707 t
ORIGIN      
        1 cgggaccgcg acgagtgtcc ccacgggtgg cgaggagacg cacgcgtcgg cgccgcgctc
       61 gccaatgtcg gttagcccca gggttccgtc acccgtcatg cgagccatcc cgccactcgc
      121 gctggcgcag ccggaccgcc cccctatggg tgggggcttg accgaggttg ggcgcgcaga
      181 accgcttgtg acggcgccaa acgcaccgcc gcgttcaaac ccggggccgc ccttggcttc
      241 accggaggca aaggctcccg agccgcacgg cacggagctc gccggaccga gatccggcaa
      301 ggcggattct ccaacgccaa gcaacgacct gtcagcggcg atgacccgcc aggactacgc
      361 gcgtgcattt gagctcgcac gctcacgggc gcagggcggc gatcgcgagg cgcaattcac
      421 tcttggttgg ctgttcgaaa agggcctaga cgatcgattc catgaaagtt gtcacaaggg
      481 agggattcat ctgtgtcttg aggcccctat atatggatca gttggatcag tagctttggg
      541 acgaggggtt gaaggacatg gcagcgtggc ggcgggcgat cgagttgtcg ctcggagatg
      601 cggagcggcg aagttgaggt cgataacgca ttcgagaacg gaaccggcaa gccgggtcga
      661 gcgggcgcgg atattgctgg cgtatcgaga ggatccgtcg ttttttgcag tggggcgcag
      721 gctcggactg catcatcaga cggtgcagcg ctgcgttgag cgggctgtgg ccgaaggccc
      781 gatggccgct ctcgacgatc gcccgcgtcc cggcaaggaa cccatgatca cccttgaggc
      841 caggacatgg ctggtgtcgt tggcgtgtcg gaaggcaaag gaattgggtt atccgcacga
      901 actttggacg acacggcttt tggccagcca cgctcgcgag catggaccgg cggaggggca
      961 tgcgtgcttg gccaggctgg cccagggcac ggtctgcaag atcctcgacc aggaggacgt
     1021 caagccgcac aaagtgcgct actacctgga acgccgcgat cccgactttg cggaaaagat
     1081 ggccgaagtg ctgtgcgtct atcgcaaggt caaacttttg aagaaggccg cggccgcatc
     1141 gcgcaagaag ccaagcgatg cagtggctgt catctcctac gacgagaagc cgggtattca
     1201 ggccatcgca acgacggcac ccgacttgcc gccggtgccg ggcgtgcacg cgaccttcgc
     1261 gcgcgagcat gagtacaagc gccacggcac ggtcaccttg atggccggca tcaatctgct
     1321 gaccggcaag gttcacgcct tggtcaagga tcgccatcgc agccgtgagt tcatcgagtt
     1381 tctcaagctt ctcgacacgg cctatccacc ccatacggcg atccacttga tccttgataa
     1441 tcattcggcg cacatctcca aggagacgcg agcttggctc gccgatcagc ctgccggccg
     1501 cttcaagttc accttcacgc ccaagcatgg gtcatggctc aacctcgtcg agggcttttt
     1561 ttccaagctg gcccgctcgg tcctgcgcca catccgcgtc tcgtccaagc aagaactcaa
     1621 ggaccgcctc atggccgcca tggatttctt caaccgggac cccgtggttc acacctggac
     1681 ctataaactc gacaaggccg cctgatatga ttcgaacctc aaaatcgatg acctagttca
     1741 tcgattccat gaaactggtc atttgcctgg gattcactcc gattccattg ccttgtaccc
     1801 tggcctttga ttcgcggagc gggggtcgaa ggtcatgacg gcatggcggc gggtgattga
     1861 gttgtcgatt ggggatgcag atgtggcgaa gctgacgtcg atcgcgcggt cgcgaaccga
     1921 accggcgagc cgggttgaac gggcgcgtat attgctgggt taccgcgaag atccgtcttt
     1981 ctttgccgtc gcgcgggctc tgggactgca tcatcagacg gttcagcggt gtattgagcg
     2041 ggcccttgcc tacggcccga tcgcggcgct cgacgatcgt ccgcggccgg gcaaagagcc
     2101 caagatcacc gccgaggcca aggcctggct ggtgtcgctg gcctgtcgga aggcgaagga
     2161 cttggggtat ccgcacgagt tgtggacgac gcggcttctg gctcaccacg cgcgtgagca
     2221 tggaccagcc gagggccacg cgtgccttga taaactggtt cagggcacgg tgtgcaagat
     2281 cctcgatgcg caggaggtca agccgcacaa agtgcgttac tacctggaat gccgcgaccc
     2341 cgaatttaag gagaagatgg cggaagtgtt gtgcgtttac cgtcaagtca aaatcctcaa
     2401 ggagaccgcg gcggtctcca agaagaagcc tgccgacgcg gttgcgatca tctcctatga
     2461 cgagaagccg gggatccagg cgattgccac cacggcaccg gacttgccgc cggaacccgg
     2521 cgtgcatgcg acctttgccc gcgatcatga atataagcgt ctcggcacgg taagcctgct
     2581 ggccggcatc gacctgctga ccggccaagt ccatgccctg gcgaaggacc gccatcgcag
     2641 tcgcgaattc attgaattcc tgcggcttct tgataccgcc tatccagccc gcacagcgat
     2701 caagttgatc ctcgacaatc attccgcgca catctccaag gagaccaaag cctggctcgc
     2761 ccaacaacct gccggccgct tcgaatttac gttcactccc aaacacggct cctggctaaa
     2821 cctggtcgaa ggcttctttt ccaagcttgc acgctctgtc ctgcgccaca tccgcgttgc
     2881 atccaaacaa gaactcaagg atcgcatcat ggctgccatg gatgacatca accagcatcc
     2941 ggtcgtccac acgtggtcct ataagcttga taaggctgca tgattatgat tcgaaccacg
     3001 aaaacgctga actagtcccc ttccggcgta tccgttccgg ccctcgggcc ccattttccc
     3061 cagggactca cgaaaggcac gatgcgctgt cggcgctgcg aggtctcgcc cgtcctctgg
     3121 gtgggcgccg ccgatgcggg cttgggatcg gttcgccggc gacgccggtc aatcgtggca
     3181 atacggagga agctatgacc cacggaagtt tcgtctggaa cgagctctat acgcgtgatg
     3241 tcgaggcggc caaggcattc tatgccgcga ccattggctg gacattcgaa ggcatgccga
     3301 tgccgcatca gaaccgcacc tactggatcg ccaggacggg gggcaagccg gtcgcgggca
     3361 tccttgacat gcgcggaatc gtgcccgata gcgagccgcc gcactggctg agctatctcg
     3421 aagtcgacga cgtcgaccga cgccttgccg agatcgagcg ccatggcggc aggatcgtcc
     3481 ggccgccgtt cgacgtcccc aatttcgggc gcatcgcaat tggggccgac gcgaccggcg
     3541 cattcttagc ctgggtgacg tcaaaacgct gaccgcgacc gccgcgacct cgctgcggcc
     3601 gcgccccaac ggcagcaccg gcgtgggcat cgccgcagat cggccggcgc gccggagcga
     3661 gcaacgcgtc cgacgccgcg aacggtgccg cttggagtgc cttgcgccgg gcgcaattgc
     3721 gaggtcgatt cagggctgtc gagagcctca ccaccctaaa gaacagtctg tcgctgcacg
     3781 acgaggcttc ccgtggatca cgagattgcg gcctctcgac gcggcatact ggaaaaggtg
     3841 tcaatcggcg tccaaaattg accccc
//
LOCUS       DATLJT010000182         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2535963,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000182 DATLJT010000000
VERSION     DATLJT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535963"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VM910_04120"
     CDS             complement(<1..268)
                     /locus_tag="VM910_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI61769.1"
                     /translation="MSTRVGFSTFAALPDELISEKISRRILSGDQGMIVWWSIGAGVH
                     VEPHSHPNEQIVWMLKGSMELRLGSEQRVCGQGDVVVIPGGTEHE"
     gene            423..599
                     /locus_tag="VM910_04125"
                     /pseudo
     CDS             423..599
                     /locus_tag="VM910_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452277.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(714..1844)
                     /locus_tag="VM910_04130"
     CDS             complement(714..1844)
                     /locus_tag="VM910_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61770.1"
                     /translation="MRHILLVVAMFIAPPLGVRAQQQAPLASLDEPCSVSADPRWTNQ
                     EKFVWERVCVGEVANFNTAPGYGGKLDPKMPDGWPQNRVLRPVFLETILLKDPYRRAL
                     HRGGVVIFGAVSLENAELDHPFGLGDSLLEKDLNLAWLRSKFPIRFQGGKVSGMLDLH
                     GLQVGSNLFMNKNEFANVNLLIASVSGQLILSGSKVTGMLNMDGLKVGSLHMDEAEFA
                     DVILVAAQVRGNLHLSRSKIGGDLECYALEVEQMILMADAKFDGRIECAWAKIKGGLM
                     MSAQFKKDVDWGEAEIGGALGLYSAQWADGTTLVLRNAKVGIMPASLIGRLGSSTFRL
                     SATPVSMSLTGSRFSSDSAPRPFHHGIRRRGGTIFGATLPSR"
     gene            complement(1908..2135)
                     /locus_tag="VM910_04135"
     CDS             complement(1908..2135)
                     /locus_tag="VM910_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61771.1"
                     /translation="MLLACWRSGQLPQQQCAFSFLRKSGLPGFEALQYYGLSAPAGTP
                     RPIVERLNKELRSMIEPLAARGRPIQARTDS"
     gene            2078..2209
                     /locus_tag="VM910_04140"
     CDS             2078..2209
                     /locus_tag="VM910_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61772.1"
                     /translation="MRKHIVAEGADPIASTPEEYAANIVREEGKWATLIKKLGLKIE"
     gene            2249..2428
                     /locus_tag="VM910_04145"
     CDS             2249..2428
                     /locus_tag="VM910_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61773.1"
                     /translation="MYSDGVVVGRIMKAMAAPVAEPWLWTLAFVHHEDRTPTHGYATT
                     REEAMAALAKSWRRE"
BASE COUNT          533 a          752 c          774 g          471 t
ORIGIN      
        1 cctcgtgctc cgtacctccc ggaatgacca cgacgtcgcc ttggccgcag acccgctgtt
       61 cgctgccgag ccgtaactcc atgctgccct tgagcatcca cacgatctgc tcatttgggt
      121 gactgtgcgg ctcgacatgg acgccggccc cgatgctcca ccatacgatc atcccttggt
      181 cgccggagag aatgcggcgg ctgatctttt cgctgatcag ctcgtcaggt aaggcggcga
      241 atgtgctgaa acccacccgt gtggacatag cttcctcccc atgagggtag ccctagcgtc
      301 gcgtcgtcac gaagtttccg cgggagcgag cccccgagga cttccgcgat ggcaccgcac
      361 agggctcgag ccttcgatca aggttgaccc ccgtaaacga ttaaatgcag ttttcgatgc
      421 gcatgctggt gcgtgctgcg aaatggtcct ggctcaaggc gtgggcgatg aaaatcgcca
      481 ggcgccgcgg catgaaaaag gccatcgtgg cgcttgcgcg tcggctggtc gtgatcatgc
      541 atcgcatctg ggttgacggt actgacttcc ggtggaccag ggaggtcgcc gcggcgtgac
      601 aactgtacag tatcaaccaa ataggaggca acctgagatc cacccaacgg tggagtgatg
      661 tccctcgcgg gacgatggat gaggtgagtt cgtatgtccg cttgaacctg ccgttacctc
      721 gacggcaagg tcgcgccaaa gattgttcca cctcgtcttc gaatcccatg atggaagggc
      781 cttggtgccg agtccgaaga gaagcgcgag cccgtgagcg acatcgacac tggcgtggcg
      841 gatagcctga aagtgcttga ccccagacgg ccgattagag aagcgggcat gatacccacc
      901 ttggcattgc gaagaacgag agtggtgcca tccgcccact gtgctgagta aaggcccagg
      961 gcgccgccta tctcagcctc gccccagtca acatcttttt taaattgagc actcatcatg
     1021 aggccgccct tgatctttgc ccaagcacac tcaattcggc catcaaattt ggcgtccgcc
     1081 ataaggatca tttgctcgac ctccaaggcg tagcactcca ggtcgccccc gatcttggaa
     1141 cggctcagat ggagattgcc gcggacctgg gcggcgacaa ggatcacatc ggcgaattcg
     1201 gcctcgtcca tgtgcagcga gccgacctta agtccgtcca tgttaagcat gccggtcact
     1261 ttggaaccgc tcaagatgag ctgcccgctc actgaggcga tgagaaggtt cacattggcg
     1321 aactcgttct tgttcataaa cagattgctg ccaacctgaa gcccgtgcaa gtcgagcatg
     1381 ccgctcactt tgccaccttg gaaacgaatt ggaaatttcg acctgagcca tgcgagattt
     1441 aggtcttttt cgagaaggct atcccccaat ccaaacggat gatcgagctc tgcgttctcg
     1501 aggctcacgg cgccgaaaat gacgaccccg ccgcgatgca gcgcgcggcg atacgggtcc
     1561 ttgagcagga tcgtttcgag aaagaccggc ctaagaacgc ggttctgagg ccagccgtcc
     1621 ggcatcttcg gatcgagctt gccgccatag cccggtgctg tattgaagtt cgcaacctcg
     1681 ccgacgcaga cgcgctccca gacgaatttc tcctgattgg tccagcgcgg gtcggctgaa
     1741 acagagcacg gctcgtcgag cgaagccagc ggcgcctgct gctgggcgcg cacgccgagc
     1801 ggcggcgcaa tgaacatggc cactacaagg agaatgtgtc gcatgacaaa tcccccaagg
     1861 acttgtacga tctagcaacc aaaaatatca gaaacggcct ttgactgtta tgaatcggta
     1921 cgcgcttgga tcggccgccc ccgcgccgcg agcggctcta tcatcgagcg cagctcctta
     1981 ttgagccgct cgacgatggg gcgcggagtg ccggccggcg ccgacagccc gtagtattgc
     2041 agcgcctcga agccgggcag cccggacttc cgaagaaatg agaaagcaca ttgttgctga
     2101 gggagctgac ccgatcgcca gcacgccaga agaatatgcg gctaacatcg tgcgcgagga
     2161 gggcaagtgg gcgacgctaa tcaagaagct cgggctgaag attgagtaag atctcgcgcg
     2221 cgatccggcg agtggaacga cgacttcgat gtactccgac ggcgtcgtgg tcggtcggat
     2281 catgaaggcg atggcagcgc cggtcgccga gccatggctg tggacgctcg ctttcgtcca
     2341 ccacgaggac cgcacgccta cccacggcta cgcgacgacg cgcgaggaag cgatggcggc
     2401 gttggcgaag agctggcggc gggagtgacg gcccgtgcac gacgagcaga tcgccaggtg
     2461 gaatcagcag cgtggaccgc gcgtggggga ctggtgcgag acgaaagatg gcggcctgca
     2521 ccgcataggc
//
LOCUS       DATLJT010000183         1720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_468320,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000183 DATLJT010000000
VERSION     DATLJT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468320"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VM910_04150"
     CDS             complement(<1..620)
                     /locus_tag="VM910_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61774.1"
                     /translation="MAALLRVSHLQTYYSSFGGARVVKAVDDVSFTLGEGETIGLVGE
                     SGCGKTTTCLSIVGLLPSAARVVGGSIAFAGEEITTKRPREMRRIRGRQIAMILQDPM
                     ASLNPLFSIYTQVAEPAYYHQAIRGRALRERVQALLRSVRIPSPAVRMREYPHQMSGG
                     MRQRVVGAIAMAGGPKLIIADEPTTNLDVTIQAQYLEVLKDIQQESG"
     gene            complement(630..1550)
                     /locus_tag="VM910_04155"
     CDS             complement(630..1550)
                     /locus_tag="VM910_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61775.1"
                     /translation="MVSTIEETRASPVPIGARDFASVPRRWTSLARQLPILPLAILVP
                     FILLALFANWIAPYDPTEPIPGAKIFEPPFWMQGGSSRALLGTDFQARDLFSRLLFGA
                     RVSLIVGVMGTIVAGSIGTALGILSGYLGGWVDQVIMRVTDAWLALPALVFAIFLATM
                     IGPSMWNIVVILGLVYWTRYARVIRGEVLSLREREFVKLAEIAGGSRARVIFRHILPN
                     VLNSTMVLASLTIGVVIIAEASLSFLGVGVPPPEPAWGSMLADGRSMLMVGDWWLTVF
                     PGLGILLVVLATQLLGDWLRVRLDPQLRNM"
     gene            complement(1558..1710)
                     /locus_tag="VM910_04160"
     CDS             complement(1558..1710)
                     /locus_tag="VM910_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI61776.1"
                     /translation="MGRLLYEGIFQRDFPLVQGVVMEAGIMIVMINLIIDLLYAYIDP
                     RIRLTK"
BASE COUNT          355 a          554 c          546 g          265 t
ORIGIN      
        1 ccgctctcct gctggatgtc cttcagcact tccaggtact gggcttgaat ggtgacgtcg
       61 aggttggtcg tcggctcgtc ggcgatgatc agctttggcc cccccgccat cgcaatcgcg
      121 ccgaccacgc gttggcgcat accgcctgac atctgatggg gatattcgcg catgcgcacc
      181 gcgggcgacg gaatgcgcac cgagcgcagc agcgcctgca cccgctcccg cagcgcacgg
      241 ccacggatcg cctgatggta ataggccggt tcggcgactt gcgtgtagat gctgaacagc
      301 gggttcaacg acgccatcgg atcctgcagg atcatggcga tctggcggcc tctgatccgg
      361 cgcatctcgc gcggccgttt tgttgtgatc tcctccccgg cgaatgcaat gctgccgccg
      421 accacgcgtg ccgcggaagg aagcagaccg acgatcgaca ggcaggtggt ggtcttgccg
      481 cagccggatt ccccgacaag tccgatcgtc tcgccctcgc cgagggtgaa gctgacgtcg
      541 tctaccgctt tgacgacgcg cgcgccgccg aacgaggagt aataggtctg caggtgcgac
      601 acgcgcagca gcgcggccat tttgcgcgcc tacatgttgc gcaattgcgg gtcgagccgg
      661 acccgcagcc agtcgccgag caactgcgtc gccagcacga cgagcagaat gccgagcccg
      721 ggaaagacgg tcagccacca atcgccgacc atcagcatcg accggccatc ggccagcatc
      781 gagccccagg cgggctcggg cggcggcacc ccgacgccga gaaagctcag agaggcttcg
      841 gcaataatca ccacgccgat cgtcaggctg gccagcacca tcgtcgagtt cagcacattg
      901 ggcaaaatgt gccggaaaat cacccgtgcc cgcgaaccgc cggcgatctc ggccaatttg
      961 acaaactcac gctcgcgcag actgagtact tcgccgcgga tcacgcgggc atagcgggtc
     1021 cagtagacga gcccgaggat cacgacgatg ttccacatgc tggggccgat catcgtcgcc
     1081 agaaagatcg caaagaccag ggcgggaagg gcgagccaag catcggtcac ccgcatgatc
     1141 acctggtcga cccagccgcc gagataaccc gagaggatgc ccaaggcggt gccgatgctg
     1201 ccggcaacga tggttcccat caccccaacg atcagcgaca cgcgggcgcc gaagagaagg
     1261 cggctgaaca ggtcgcgggc ctggaaatcg gtgccgagca atgcacggct gctgccgccc
     1321 tgcatccaga acggcggctc aaagattttc gcccccggga tcggttcggt tgggtcgtaa
     1381 ggggcgatcc aattggcgaa cagtgcgagc aagatgaacg ggaccaggat cgcgagcggc
     1441 aagatgggca actgccgcgc caggctggtc cagcgccgcg ggacgctcgc gaaatctcgc
     1501 gcaccgatcg gaaccggaga ggcacgtgtc tcttcgatgg tggagaccat gacgcaccta
     1561 cttggtcaag cgaatgcgcg gatcgatgta ggcgtaaagc agatcgatga tcagattgat
     1621 catcacgatc atgatcccgg cctccataac gaccccctgt acgagcggaa aatcgcgctg
     1681 gaagatcccc tcgtagagca gccggccgat gccgggccaa
//
LOCUS       DATLJT010000184         3940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_8781,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000184 DATLJT010000000
VERSION     DATLJT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8781"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VM910_04165"
     CDS             complement(<1..1076)
                     /locus_tag="VM910_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61777.1"
                     /translation="MRYSPLIGLFCIGLSLNSIADAQSPSVVVRIGILNDQSGPYADF
                     GGKTSVDAARIAVEDVGGKVLGKSIEIIIGDHQNKPDVASAIARRWFDVDGVSAIAEL
                     TNSAVALAVQQIAKQQGKITLFIGPATTRLTNEDCSPTGFHWAFDTYSQAVGTARAVV
                     AEGGKSWFLLVADYAFGHQMANDLTKVVKTSGGTVVGEVRHPLNTSDFSSFLLRAQSS
                     KAQIIGLASAGADTINSVKQAAEYRIAAGGQKLAGLVVVISDIDALGLTTANGLVFTT
                     AFYWDRDEAARDWSKRFFERTGRMPGMVQAGTYSAVLHYLKAMQAAGTADGKAVADKI
                     RELPVDDFFAKGRVRLDGRMMHDM"
     gene            complement(1129..2409)
                     /locus_tag="VM910_04170"
     CDS             complement(1129..2409)
                     /locus_tag="VM910_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI61778.1"
                     /translation="MAAAFLAMNEIYGNVFQVEAAEAHEPELMLARAQSLAGQFVFDV
                     QTHFVRDDFKHEELLGLASFASEYWNPKMKEEGVSSLARYKFQNYVKEIYYDSDTSLA
                     LLSGAPFDDPSWWLLSNEQIVKAREIINDFAGSRRLLAHSVITPKQPGWMEEVDKAIA
                     VYKPDSWKSYTIGDPLAPSKFPWRLDDEQVMYPFYEKAVNASITTICIHKGLLPPDYE
                     KSFAGVWEYATAWDIGKAAKDWPQMNFVVYHSALRPFLELPDRAWAEFESTGRIRWAS
                     DLAEIPQRFGVTNVYAELGTSFANSAVAHPKFSAALIGTLIKGMGADHIMWGTDSVWY
                     GSPQWQIEAMRRLEIPEDMQKKYGFPALGGANSATKQLIFGANAARLYRINLKAADTR
                     MPAYSEDRLASLKSEYELAAKTPSNLRYGYVRAA"
     gene            2942..3235
                     /locus_tag="VM910_04175"
     CDS             2942..3235
                     /locus_tag="VM910_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HVI61779.1"
                     /translation="MPTFMMSLNWTDQGIRAVKDSPKRSEDARELAKKVGVEIKHLYL
                     TSGDSDLVIFVDSPSGDNVAKFALAVSSLGNVRTRTVRAWSADEYRKLISELP"
     gene            complement(3641..>3940)
                     /locus_tag="VM910_04180"
     CDS             complement(3641..>3940)
                     /locus_tag="VM910_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61780.1"
                     /translation="FRILGVMSAERSPFIPNGPTFREQGFNEVWSVSRGIAAPAGLPK
                     DVEALLIGYLEKTISSKEHKAKAEALALDPQIIKGDDYRKFLKDNEQATKKLMGW"
BASE COUNT          766 a         1183 c         1128 g          863 t
ORIGIN      
        1 tacatgtcgt gcatcatgcg gccatccaaa cgcacccgcc ctttggcgaa gaaatcgtcg
       61 accggcaact cccttatttt gtctgcgact gccttgccgt ctgccgtccc cgctgcctgc
      121 atcgccttga gatagtgcag caccgctgaa taggttccgg cctgaaccat tccgggcatc
      181 cgaccggttc gctcgaagaa ccgtttcgac caatcccgag ccgcctcgtc ccgatcccaa
      241 tagaaagccg tcgtgaagac tagtccattg gccgtcgtca ggcccagcgc gtcgatgtcg
      301 ctgatcacga ccacgaggcc ggccagtttc tggccgcccg cggcgatgcg gtattcggcc
      361 gcctgtttca ccgagttgat ggtatcggcg ccagcactcg ccaggccgat gatttgagcc
      421 ttcgagctct gcgcccgtaa cagaaacgat gagaagtcgc tggtgttgag tggatgccgg
      481 acttcgccaa ccacggtacc gccgctagtc ttgacaactt tggtgagatc gttggccatc
      541 tgatgaccaa aagcgtagtc ggcgaccagc aggaaccagc tcttgccgcc ctcggcgacg
      601 acggcacggg ctgtgccgac cgcctgcgaa tacgtgtcga acgcccaatg aaagcccgtc
      661 ggcgagcaat cctcattggt gagccgcgtt gtcgccggcc cgatgaacaa cgtgatcttc
      721 ccctgctgct tggcgatttg ctggactgcc agtgcaacag ccgaattcgt cagctcggca
      781 atcgcactca ccccgtcgac gtcaaaccaa cgtcgcgcga tggccgaggc cacgtccggt
      841 ttgttctgat gatcgccgat gatgatctca atcgattttc caaggacttt gccgccgaca
      901 tcctctacag cgatgcgcgc ggcgtcgact gaggtcttgc ctccgaaatc tgcgtagggc
      961 ccggattggt cgttgagaat tccgatgcgt acgacgacgc tggggctttg tgcatccgcg
     1021 atgctattga gagatagccc gatgcagaac aggccaatta aaggcgaata tcgcattgat
     1081 ccctcccaaa aggcggtgca gtactggcat atttcgcccc ggtcagtgtc acgcggcgcg
     1141 gacgtaaccg taacgcagat ttgagggggt ctttgccgca agctcgtact cactcttcaa
     1201 gctggccagc cggtcctcgg aataggccgg catccgcgtg tccgccgcct tgaggttgat
     1261 ccgatatagg cgggccgcgt ttgcgccgaa gatcagctgc ttagtcgcac tgttggcacc
     1321 gccaagggcg gggaagccat atttcttctg catgtcctca ggaatctcaa gccgccgcat
     1381 ggcttcgatc tgccattgtg gcgacccgta ccaaacagaa tcggtgcccc acatgatatg
     1441 gtcggcgccc atccccttga tcagcgtgcc gatcaacgcc gccgagaatt tgggatgggc
     1501 cacggccgaa ttggcaaacg acgtgccgag ctccgcatac acattggtga ccccgaacct
     1561 ttgcggaatc tcggccagat cgctggccca tctgatgcga ccggtcgatt cgaattccgc
     1621 ccaggcccga tcaggcaact cgaggaacgg gcgcagcgcg gaatgataca caacgaaatt
     1681 catctgcggc cagtccttgg cggccttgcc gatatcccag gcagtcgcgt attcccagac
     1741 gcctgcgaac gatttctcgt aatcgggcgg cagcaggccc ttgtggatgc atatcgtcgt
     1801 aatgctcgcg tttaccgcct tttcatagaa cggatacatc acctgttcat catcgagacg
     1861 ccaaggaaac ttcgatggtg cgagtggatc gccgatcgtg taggacttcc acgaatcggg
     1921 tttgtagacc gcgatcgcct tatcgacctc ctccatccag ccgggctgct tgggggtaat
     1981 gactgaatgc gccagcaatc gtcgtgatcc cgcgaagtca ttgatgattt cgcgcgcctt
     2041 gacgatctgc tcgttggaca agagccacca ggatgggtca tcgaatggcg cgccgctcag
     2101 cagcgccaaa ctggtgtcgc tgtcgtagta aatctccttc acgtagttct ggaacttgta
     2161 gcgggcgagc gacgacacgc cctcttcctt catctttggg ttccagtact cgcttgcgaa
     2221 agaggccagg cccaggagct cctcgtgctt gaaatcgtcg cgaacgaaat gagtctggac
     2281 gtcgaaaaca aactgtccag ccaaactctg cgcccgcgcc agcatcagct ccggctcgtg
     2341 ggcctcggcc gcttcgacct ggaatacgtt gccatagatt tcgttcatcg cgaggaaggc
     2401 tgcagccatg ccgcagctcg ttttcataaa ctgccgtcga ctgaggccaa cccgcttgct
     2461 gctgacgtcg gccagctcca gcatccggcg ttcgacgcgc ttttgtgtct cactctgcgg
     2521 aggcgggagg tattctccat tggacacgat ctgtgtcggt atgggggttg cggcagaaat
     2581 cgcttcagca ccggccacta gacgctgttc tcgttcgctg agccaactag tcattggcgt
     2641 cctcctttaa cctcgctggc tgctgcgcgt gcgcgacgag cggccacgcc gctgccgcgc
     2701 acgcgagaag ggtgatgaac ccgcggcgct tcatttgatc gaattgcatt tcggtccccc
     2761 gccagccagg gccggatggc aggataccga attgacgagg atcagtcagg aggtaatgga
     2821 acggttttgc ttggcttgtt gctaagacag tacagttgac ccacatcaca tcgacttaac
     2881 cgacttgtaa ttgcaaaaac atcggctcat ggtcgcgccc acaatatcga gggaggacct
     2941 catgccgacc tttatgatgt cgctcaattg gacagatcaa ggaatccgcg cagtcaagga
     3001 ttcgccgaag cgctcagaag acgcgcggga gcttgccaaa aaggtgggcg tcgaaatcaa
     3061 gcatctttat ctcacgtcgg gtgacagcga tctcgtcatc ttcgtcgatt caccgagtgg
     3121 tgacaatgtc gctaaatttg cgctggcggt tagctcactt gggaatgtgc gcacccgcac
     3181 tgttcgcgca tggtcagcgg atgaatatag gaagctcatt tccgagctac cttaagcgtt
     3241 tgagggtcag ttgtatcaac tgtcgttgcc cagccccgct acgaagaatg ggggaacggt
     3301 catctgcaac acgctgcgtt cgattctaat tcgactcgtc gcctacgttg ctgtcgcgat
     3361 cgctgccggg cttttgtttc tggctgtggc aacggtctat gagttgatgt ggccgaggcc
     3421 cgtggtcccc ctctcgcaaa tgagtgacag tccgtagaaa tattttttga attcatccgt
     3481 tggccggctt agcgttgtgg cgcaggcgat agccgtcacg cggcgcgcgt ctcattcgat
     3541 cacctcgtcg gcacggacga gctgcgccgc aggcacgtgc cgggcacgcg ccttgctgtc
     3601 ttgagatgat gaccaattcg tacttggccg tgcgccgcgg ctaccaaccc atcaatttct
     3661 tggtggcctg ttcgttgtct ttgaggaatt tgcggtagtc atcgcccttg atgatctgcg
     3721 ggtcgagcgc caacgcctcg gccttggcct tgtgctcctt cgagctgatg gtcttctcca
     3781 ggtagccgat cagaagggcc tcaacgtcct tcggcaagcc ggccggcgcc gcgatgccac
     3841 gcgacaccga ccacacctcg ttgaagccct gctcgcggaa ggtcggcccg ttggggatga
     3901 acggcgagcg ctcggctgac atcacgccga ggatgcggaa
//
LOCUS       DATLJT010000185         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2995587,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000185 DATLJT010000000
VERSION     DATLJT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995587"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VM910_04185"
     CDS             <1..252
                     /locus_tag="VM910_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HVI61781.1"
                     /translation="GENMLVIHPDECIDCGVCEPECPVDAIKADSEPGLEKWLSLNAE
                     YARIWPNITVKKAPPSDSKEWEGKPDKLQYFSPNPGAGD"
     gene            787..1233
                     /locus_tag="VM910_04190"
     CDS             787..1233
                     /locus_tag="VM910_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61782.1"
                     /translation="MDVELIALGERFEKLLPEHMDAWLAWAPRMRAAHAEVRDNTAAL
                     AVAIQRTGCDVAQARMSELERDMQPLAEEIIAAPATSLGGLRAKALVALWEAFPAHAS
                     HDGAFDFPDASRSLFDAVAEMIGLMPMVRELEARLAADVKFKDVEA"
     gene            complement(1815..2324)
                     /locus_tag="VM910_04195"
     CDS             complement(1815..2324)
                     /locus_tag="VM910_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61783.1"
                     /translation="MSRSLIASLLLTARRKCETAIREATPGAWTIARVKARTKPVPAI
                     VPIAPSLPTLAVAIAPPSSEIAIRASVQEIDLFDFIAGQGQELALADGDDLQMGLEQR
                     IIPRPQRGQETIASMLVSIWIHVGPTSPTSHANSLASSGNKPIPGHVAKGTRFYMSSQ
                     QYLKNQERK"
     gene            complement(3127..>3305)
                     /locus_tag="VM910_04200"
     CDS             complement(3127..>3305)
                     /locus_tag="VM910_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="HVI61784.1"
                     /translation="PPTQDRRRLSRYMRRWLVERFFAWIQWQRRILVRWEYYAQNFLG
                     FVQLACLVGLFRRF"
BASE COUNT          679 a          955 c         1033 g          638 t
ORIGIN      
        1 ggcgagaaca tgctcgtcat ccatcctgac gaatgcatcg actgtggcgt atgcgaaccg
       61 gaatgccccg tcgatgccat caaggctgat agcgaacccg ggcttgagaa atggctgtcg
      121 ctgaatgcgg aatacgcaag aatctggcca aatattacgg tcaaaaaagc gcccccgtcg
      181 gactctaagg agtgggaggg gaagccggac aaactccaat acttctcgcc aaatcccggc
      241 gcgggtgatt gacgggctta tgacgctcta cggctatcga cttctgcgct gtaacgcccg
      301 cgcaaccatc gataggcccc ctcacggcag acctgaacct tccggccgac tttttccgcc
      361 gttccttgag ttgattgccg gtcagcggag ccgcgatgac cttcaggtcg atcgcttcgg
      421 gcatcatgat gtccaagccc cgcgctacct attcaattgc tgctgtatct gccggcacat
      481 tttacgagtt tgaccattag ctcgaccgag atgccatgcg ccgctaggat cgcctcggtc
      541 gcgccgagag ccggcgagca gctcaagcgc gcggcgggga gttgcgggag gaatgcgctt
      601 gcgccgccgt ggcgcgggtt gcctaccggg gacattataa gcactgctta cgcgagcatg
      661 aggcgcgagt cgagctgtgg aagcagcagg cgctcggcgg ctaggcaagc cggggccggc
      721 ctcaccctgg gctatgacgc ccagccggct ccgggatccg gacaaggagt agccatgtct
      781 gcagccatgg acgtcgagct gatcgcgctc ggtgagcggt tcgagaagct cttgcccgag
      841 cacatggatg cttggctcgc gtgggcgccg cggatgcggg ccgcgcacgc ggaagtcaga
      901 gacaacacgg ccgcgctcgc cgtcgcaata cagcgcaccg gctgcgacgt ggcccaagcc
      961 cgcatgtcag agcttgagcg tgatatgcag ccgctcgccg aggagatcat tgccgcgccg
     1021 gcaacatcgc tcggcgggct gcgcgctaag gcgctcgtgg ccctctggga ggcatttccg
     1081 gcccacgcct cccacgatgg tgcgttcgac tttccggacg catcacgctc gctgttcgat
     1141 gcggtcgccg agatgatcgg cctcatgcca atggtgcgcg agctcgaggc ccggctcgcg
     1201 gccgatgtga agttcaagga cgtcgaggcg tagcatgatg cgaaggcgcg ggggccgatc
     1261 gctgcgcgaa acggcgagaa gtctcgggcc tttccactgg gtggcatgtt agaaaagatt
     1321 gtcgtggctg gcagttcagc agccggccaa atcagcgagg accctatgcc ccgatataag
     1381 tgcaaaagca ttgctgcgct cgacatagct ctcggcggct tgccagccaa gatgcaagtt
     1441 gaggcggacg caggcaccgg cgtgtctgca aagactgttg gagaacttcg gaaggtgacg
     1501 acgtggtctg agagtttggt gataacaacc ccgcatgaac gccatcctga aagcgcagta
     1561 aagatcagca aggctagcgt agcgactcgc acctcgccga aaccgtaggg tgcggcgcga
     1621 cagacaaacg ctaccaggcg cttgcaacct gcgccgcggc cggaccttcc tcaatccgtc
     1681 gtggcagatg acgggagttt ggactatcct cttacacggg cgcgcgtagc ggcgataatc
     1741 tatttttggc gtcagtactt tcatgggggg ttgtgggcgc ccccagttta cggaagctga
     1801 aaaggtgaat ggttctattt gcgctcctga tttttgaggt attgttgcga gctcatataa
     1861 aaccgcgttc ctttagcgac gtgccccgga atcggtttgt tgcctgagga cgcgagcgaa
     1921 ttggcgtggg atgtcggcga ggtcggccca acgtgaatcc agatcgagac cagcatcgac
     1981 gcaatcgtct cctggccgcg ctgcggccgc gggattatgc gttgctcgag ccccatctgc
     2041 agatcgtccc catcggcgag ggcgagttcc tgcccctgcc cggcgatgaa atcgaacagg
     2101 tctatttcct gcacagaggc cctgatggcg atctccgagg acgggggtgc aatcgccacc
     2161 gcgagcgtgg gaagcgaggg agctatcggt acgattgcgg gaactggctt cgtgcgcgcc
     2221 ttcacgcgag cgatggtcca ggcgccgggg gtcgcctcac ggattgcggt ctcgcacttc
     2281 cgtcgcgccg tgagcaagag cgaggcgatc aatgatctac tcacccgcta tcacatggcg
     2341 ctgatgtgcc aggttcagca aacctcgttg tgcaattcgc tgcacgatgc gacctcgcgc
     2401 ttgagccggt tgctgttggt tctgagtgag ctaggacgac accatttcgt tcagctagga
     2461 acgggttggc tgatatgcta gggctgcgcc gatcgagcgt gacattggcc gcacagacac
     2521 tgcaatcgcg gcgcttcata agctttcgac gcggacgaat cgagatcatg aaccgcaagg
     2581 ggctgaaagc cgctgcgtgc gaatgctacg aggtcgttag ccggacattc gatcaatttt
     2641 tggaggacat ttatggcggg cagcaccgac ggtcttctaa ttcaccgccg cgatgttaac
     2701 gcgtggcggg ctcctgcgcg ggcctgcctc gcgagccgga aagatcatta aggacaattg
     2761 ccagtgcact ggccgcgacg cagaacaacc ctattcgctc gccgtcagcc gttcaaattc
     2821 aacgatctac gtcacaacat gtgtcggacc gtaaatcgct ctcggtctaa ccgagaccca
     2881 aagacgggat gggcactcaa atgtgaacgg tgtgacggta aaggtggcat atccgttcca
     2941 gaaaactaaa gctcgcaggg gctgctttgg atcaaactta cggcttctcc cagtacgcct
     3001 tcgcagcatt caggaaggtc aagaagccgg ccgtcgccca agatgtcggc tggttatcgc
     3061 gcaccagcca atgcaaatga ttggcgcaga ccaattcgtc tcgttgcgca gtcactagaa
     3121 cctatctcaa aatcgcctga agaggccaac gaggcaagcg agctgtacga aaccgaggaa
     3181 gttctgggcg tagtattccc aacggacgag gatgcggcgc tgccattgaa tccaagcaaa
     3241 gaagcgctcc acgagccagc gtcgcatata gcggcttagc cgtcgccgat cttgcgtggg
     3301 cggct
//
LOCUS       DATLJT010000186         1579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3684625,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000186 DATLJT010000000
VERSION     DATLJT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684625"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VM910_04205"
     CDS             <1..202
                     /locus_tag="VM910_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61785.1"
                     /translation="KRAPSKAAKAAALLDLIRKVRNKMLATIDLATAMPHELQRLLAI
                     DRYESRARTRRRRASAKLEQDG"
     gene            complement(292..1290)
                     /locus_tag="VM910_04210"
     CDS             complement(292..1290)
                     /locus_tag="VM910_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61786.1"
                     /translation="MNDEKTTGGFMIGLLRTALFGLSCLALTAGPCGAGDYPNRPVHF
                     INGFAPGGPVDTTARIMGQWLSDRLGQQFVIENRTGSGGNIAAAAAISSPPDGYTVLF
                     VGANNAISTSLYKHLPFDFLHDTVPVAMFMMVPNMLVVANAMPVKTVQEFIDYCKANP
                     GKISYASSGNGTSVHMTAELFKAVTKCDMLHVPYRGSALAYPDVISNKVQLIFDNLPG
                     ALEQAKGGSVRALGVTSAKRWPGVPDVPAIAETVPGYDATVFYGMTAPKGTPPEIVDL
                     LNKAVNEALNDPKLVARLAEMGGVPKPMTPAEFGKYMADETEKWRKVVEFAGVSVD"
     gene            1391..>1579
                     /locus_tag="VM910_04215"
     CDS             1391..>1579
                     /locus_tag="VM910_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunogenic protein precursor"
                     /protein_id="HVI61787.1"
                     /translation="MKHAAIICAGILLLTAGAAAQQGDKAIPKTTITLGTATPGGGFP
                     LYGNAFAEIMNAADPTLSI"
BASE COUNT          295 a          494 c          502 g          288 t
ORIGIN      
        1 caagcgtgcg ccttcgaaag ccgccaaagc agcagcgctg ctggacctga tccggaaggt
       61 tcgcaacaag atgctcgcaa ccatcgacct cgccaccgcc atgccgcacg agttgcagcg
      121 gttgcttgcc atcgaccgct atgagagtcg cgcccgaacg cggcgccggc gcgcctccgc
      181 caagctggag caggacggct gacgcgattt tttgcaaaac gaacccaatt tttcgtcctg
      241 cgttcagaag gatagatccg caacctgagc gaacgcctgg cgccgcgccg gttagtccac
      301 cgacacgccg gcaaactcca ccactttgcg ccatttctcg gtctcatcgg ccatgtactt
      361 gccgaattcg gccggggtca tcggcttcgg aacgccgccc atttcggcaa gccgcgccac
      421 gagcttcggg tcgttcaggg cctcgttgac cgccttgttg agcaggtcga cgatctccgg
      481 cggcgtgccc ttcggtgccg tcatgccgta gaacacggtc gcgtcgtaac cgggcacggt
      541 ctcggcgatc gcgggaacgt cgggcacacc tggccagcgt tttgccgagg tgacgccgag
      601 cgcgcgcacg ctgccgcctt tcgcctgctc caatgcgccg ggcagattgt cgaagataag
      661 ctgcaccttg ttggagatga cgtcgggata ggcgagcgcg gagccgcggt atggtacgtg
      721 cagcatgtcg cacttggtca cggccttgaa caattccgcg gtcatatgca cggaggtgcc
      781 gttgccggat gaggcgtagg agatcttgcc ggggttggcc ttgcagtagt cgatgaactc
      841 ctggaccgtc ttcacgggca tggcattcgc cacgacgagc atgttcggaa ccatcatgaa
      901 catcgcgacc ggaacggtgt cgtgcaggaa atcgaaggga aggtgcttgt aaagcgaggt
      961 gctgattgcg ttgttggcgc cgacgaacag caccgtatag ccgtcgggag gcgaagagat
     1021 cgcggcggct gctgcgatat tgccgccgga gccggtgcgg ttctcgatga cgaattgctg
     1081 gcccaggcga tcggacagcc actggcccat gatccgcgcc gtggtgtcga ccggtcctcc
     1141 cggcgcgaag ccattgatga aatgcaccgg ccggttcgga tagtcgccgg cgccgcaggg
     1201 accggcggtg agggcgaggc aggaaaggcc aaacagggca gtacgcaaca atccgatcat
     1261 gaaccctccc gtcgtctttt cgtcgttcat ccttgcgcgc atgcttccat aaaacccggg
     1321 gcttgcctac aagcccggtt gcgtcatgat taggcattct gtcgcgcggg aggccggctt
     1381 gaggtcgagc atgaaacacg ccgccatcat ctgcgccggc attctgctgc taacggccgg
     1441 cgccgcagcc caacaggggg acaaagccat tccaaagacg acgatcacgc tcggcacggc
     1501 gacgcccggc ggcggctttc cgctctatgg caatgccttc gccgagatca tgaacgcggc
     1561 cgatccgacg ctgtcgatc
//
LOCUS       DATLJT010000187         8083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_8881,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000187 DATLJT010000000
VERSION     DATLJT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8881"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VM910_04220"
     CDS             complement(<1..1271)
                     /locus_tag="VM910_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVI61788.1"
                     /translation="MSRPTGGKTAKDDVNRRRNLGFFFDQSVARLPDKVAIIDLFGAR
                     ERCATYRQLDERMNGVASMLARLGVTPGERVAMLIGNRTEFIEFFFGSMRAGAIPLPL
                     NTRLAAGTLDAIIVEAACVLVVVDPSSHRDALAIAQRLPLRQRLLLDARQEGFLSFEE
                     EMAKPAVPVDPPALADDAQAFQPYTSGSTGRPKGAIMTHHGMLWYVAYNQRYWPSAES
                     DRGLVALPLFHKNALRGTVKPMLYAGGSFVLMRAYEPRAYLDALAKYKCTYSRGVAAV
                     FTMFLQHRDYLRTLDLSALRDMTIGSAVVTPELVDAVERALPHVKVSESYGLTEGGSP
                     LRAPIDGRAVPRGSPGVPAPEIELRLIDAEGNDADEGELLIRCPYVCRGYYNEPEITR
                     AKLTDGWLHTGDIFCKDKDGFFYFRSRVDDM"
     gene            complement(1507..2199)
                     /locus_tag="VM910_04225"
     CDS             complement(1507..2199)
                     /locus_tag="VM910_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HVI61789.1"
                     /translation="MPRQRPQRPKLAARETSLYLPVKRFLESLGFEVKGEICGCDLVA
                     VRGDEPPLVVIGELKLGFNLELVLQGINRMAACDQVWLAVRFSGRRGRERDPRVRKLC
                     RLLGFGLLGVSTSGRVEVLVEPRPWRPRRDPRRRSRLVDEHRRRLGDPVLGGSCKAPI
                     MTAYRQQALACAASLSSGPRRTSEIKTAIPDAPKILLRNVYGWFFRVERGKYALTQEG
                     TAALSRWPQPQA"
     gene            2585..2887
                     /locus_tag="VM910_04230"
     CDS             2585..2887
                     /locus_tag="VM910_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVI61790.1"
                     /translation="MAGKATTPSRPVTLKHLAARLAADHELTKREGEAILGDLVSLIT
                     KHLKKGERIRIAGFGILQVRQRAARMGRNPATGEAIKIKASKKIAFRPAKHLKMAV"
     gene            complement(3050..3328)
                     /locus_tag="VM910_04235"
     CDS             complement(3050..3328)
                     /locus_tag="VM910_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61791.1"
                     /translation="MSDATESDDERPCLHCLIGDVIDDFYAEYGSLSGEKDTIDVDEI
                     ITALAKTVAEMTFGSDAALRQRILEDLTREISQFEAEFANAPPSEVRH"
     gene            3486..3812
                     /locus_tag="VM910_04240"
     CDS             3486..3812
                     /locus_tag="VM910_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61792.1"
                     /translation="MTRNGYFQALANPEDDNPRWPPYLIWTMLRPAYLSQKLDGPLPT
                     KDGGTLRTIREACDYMTGMGKKRELCQHWQRACKLILAQADVVTVRALELALFYDAKL
                     DVAPMA"
     gene            complement(3960..6395)
                     /locus_tag="VM910_04245"
     CDS             complement(3960..6395)
                     /locus_tag="VM910_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61793.1"
                     /translation="MLTLRDQVNAAIPKRAKNLDGLIANKRHHDANPNSDHEPWVKDC
                     GVGVVTAMDITHDPKGGFDSYKFANYLLQIRDPRIKYIISNGRISSGSGQAHPAWQWR
                     SYKGSNKHDHHIHISVKSTKDLYDSTKPWDLEVFFNKAKPSPVQPVEPLIVTRSDSTE
                     ANREHMGQLILDFEARRDKKGHLAIYPLPRNDRGGFYEVAGINDGYHPEEAAELAKLI
                     HAGRYNDAEKRAREIIIEYTNPVAGWYQHPAVEFFLRDTAFNRGPGAPRTLKRNAREI
                     AGLPEQAEEVMPKFEVRDIGLPGPASGSSWSVPGRLRNDFVIETYETDPADKATDSEA
                     AYISKLKDTLTMLKDAERQDTHFFGSSVHQARTDHRETEVCELRKIATEFADGRIDAD
                     AAASATTEVAQKYAQNCPDLVAQDKAAAELRFAGDFTITKNYAPDGPVKEYIDRIADI
                     LDRLAEDEVADKTAGDSVARLDARQGYRKEAANSLRKEFEEFRRGSVAVRIAADHALV
                     SQGIYIGKRDQLKARLFNVIPKYKDEPKRVVELEFKVTPGLPPPDDQPSQEKQDLFVQ
                     VGNADTVISTVSRHMDDRAEKIEKRWWYGPKQRRKRRAEAERQLAQRLLDDYLRKLAG
                     IAVVGLEGPHTTLAKLALNELRSEFTAQQAGRIKNIYVRGLGATAATAAVPLLLLYAF
                     IAKVVGSGWWYDHRAFLIAAAGAAIGTWLSFSIRRVSLSFSDLGILEEDLLDPSVRII
                     FVIALTLTACLLFWTGVMNIQIGDLKTNAAEFMQRGSIAMLLGVFFGISERALATAIS
                     GRATAFVRSVGGG"
     gene            complement(6437..6577)
                     /locus_tag="VM910_04250"
     CDS             complement(6437..6577)
                     /locus_tag="VM910_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61794.1"
                     /translation="MASYIQRRKFLATLDGAAVAWPLAARAQQHEPRGWICTPLSTGQ
                     RL"
     gene            complement(7235..7311)
                     /locus_tag="VM910_04255"
     tRNA            complement(7235..7311)
                     /locus_tag="VM910_04255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7275..7277),aa:Arg,seq:cct)
     gene            7575..>8083
                     /locus_tag="VM910_04260"
     CDS             7575..>8083
                     /locus_tag="VM910_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61795.1"
                     /translation="MPLTRRHFIGTAASAAALACQCVEAQEEHALFWRIEVPDGGRGV
                     AFGYTRVAAAVVPEVVKDGVRLMEGAKRVVLDMNNTRFPAMKIDPNLPSLWPKLDPAR
                     AEQVRSILTSMHVPPAQIENLPGFFIASLLYAEGQANPVPSVGGVIVDQAKALGRPMT
                     VLLEASDVE"
BASE COUNT         1586 a         2505 c         2369 g         1623 t
ORIGIN      
        1 aacatgtcgt cgacgcggct gcggaagtag aagaagccat ccttgtcctt gcagaagatg
       61 tcgccggtgt gcagccatcc gtcggtgagt ttggcgcgcg tgatctccgg ctcgttgtaa
      121 tagccgcggc agacgtaggg acagcggatc agaagctcac cttcatcggc gtcgttgccc
      181 tcggcgtcga tcagcctgag ctcgatctcg ggcgcgggca cgccggggct gccgcgtggg
      241 accgcacggc cgtcgattgg cgcgcgcagc gggcttccgc cctcggtcaa gccataactt
      301 tccgagacct tgacgtgcgg aagcgcgcgc tcgacagcgt cgacgagctc gggcgtgacc
      361 accgccgatc cgatcgtcat atcgcgcagc gcactgagat cgagcgtgcg cagatagtcg
      421 cgatgctgga gaaacatcgt gaataccgcg gccactcccc gcgaataggt gcatttgtac
      481 ttggccaagg catcgagata ggcgcgcggc tcgtaggccc gcatgagcac gaaggagccg
      541 ccggcgtaga gcatcggctt caccgtgccg cgcaaggcgt tcttgtggaa gagcgggagc
      601 gcgacaagcc cgcggtcgct ctcggcggag ggccaatagc gctggttgta ggcgacgtac
      661 cacagcatgc cgtgatgcgt catgatcgcg cccttggggc ggccggtcga tccggacgta
      721 taaggttgga acgcttgcgc atcgtcggca agagcgggcg ggtcgacggg gaccgcgggc
      781 tttgccattt cctcctcgaa ggacagaaag ccctcttggc gcgcatcgag cagcagccgt
      841 tggcgcagtg gcaaacgctg cgcgatcgcg agcgcgtcgc gatggctgga cgggtcgacg
      901 accacgagca cgcacgcggc ctcaacgatg atcgcatcga gcgtgccggc ggcaagccgc
      961 gtgttgagcg gcagcgggat cgcgcccgcg cgcatcgatc cgaagaagaa ttcgatgaac
     1021 tctgtgcgat ttccgatcag catggcaacg cgctcgccgg gcgttacgcc gagccgggcc
     1081 agcatgctcg cgacgccatt catgcgctcg tcgagctgcc gataggtcgc gcagcgttcc
     1141 cgtgcgccga acaaatcgat gatcgcaacc ttgtccggca ggcgcgccac ggattgatcg
     1201 aagaaaaagc caaggttgcg ccgacgattc acgtcgtcct tggccgtctt acctcccgtc
     1261 ggtcgcgaca agtcaaaccc cgctgtgacg aaaaagccac acgtattcca aatatcaaat
     1321 cgtggccgaa tttccggttg gcagggcggg caaatcaggt caccttactc gcgcctaatc
     1381 atgggcgttt cctccttcac taagccccgc ttgccggggc ttttttcttg gccagcgaac
     1441 gcgcacacca ggcctgcccg cccccgcgcg gacggcgccg atatcaccac agcaccgcca
     1501 aaacgactac gcctggggct gcggccaacg cgacagtgcg gccgtccctt cctgcgtaag
     1561 ggcatacttg ccgcgctcga cgcggaaaaa ccagccgtag acgttgcgca gcagaatctt
     1621 cggcgcatcc ggtatagcgg ttttgatctc ggatgtgcgt cgcggaccgc tagacaggga
     1681 cgccgcgcac gcgagcgctt gctggcggta tgcggtcatg atcggcgcct tgcagcttcc
     1741 gccaaggacc ggatcgccga gacgccgccg atgttcgtcg acaaggcgcg agcggcgccg
     1801 tggatcgcgg cgaggccgcc aaggcctggg ctcgaccaac acttcgacgc gaccgctcgt
     1861 cgacacgccg agcaaaccaa aaccgagcaa ccggcaaagt ttgcgcacgc gcgggtcgcg
     1921 ctcgcggccg cggcgtccgc tgaaacgcac ggcgagccag acctgatcgc aagctgccat
     1981 ccggttgatg ccttgcagca ccagctcgag attgaagccg agcttcaatt cgccgatgac
     2041 gacgagcggc ggttcgtctc cgcggaccgc gacgagatcg cagccgcaga tttcgccctt
     2101 cacctcgaag ccaaggctct cgagaaagcg cttgaccggg aggtagagcg aggtttcccg
     2161 cgctgcgagc ttcggccgtt gcggtcgttg acgtggcatg ggtacccggc gacgcgaaca
     2221 tcgggcctcg cccgcgtccc acaatcgtgc tgctgccgca accatgtcgc caacgtggta
     2281 agcgcttcgt taacgcgagc cgcgcgcggc gcaaaccagc atcgcgaccg acgtaagcgc
     2341 tcgcggcctg ggtgtgcgcc gcccgaaacg gaacaacgtt cccgttgcga ttccccttcg
     2401 ttcccggatc gattccgtaa tgtgccgtca aggtgcactc caaaagcgca cggctcgctt
     2461 ctcgcacgat cgcgaaaacg cctgtgtttt ggccacttca ggcgcaaaga tcgctttcct
     2521 tcctgtctcg aatcatatct cttagcattt gcgcatttcg cgcctctgag gagggcaaac
     2581 catcatggcg ggcaaagcga cgacacccag ccgccccgtt acactcaagc atttggcggc
     2641 aaggttggcg gccgatcatg aactcacgaa acgcgagggc gaggcgatcc tcggcgatct
     2701 cgtgagcctg atcaccaagc atctcaagaa aggtgagcgg atccggattg cgggttttgg
     2761 catcttgcaa gtcagacaac gcgcggcccg catggggcga aaccccgcga ccggcgaagc
     2821 catcaagatc aaagcgagca agaagatcgc attccggccg gccaagcact tgaagatggc
     2881 ggtttaaccg ctggcgcaga tacgtgagac ctgccccgtc ggccaccgcc ggcgggggca
     2941 tatctcgtct ggtccgattg cgccaagcgt ttccacagcg atagtcgatc ggtcgccagc
     3001 gctggcgttc tcgatccgat agggcatgac caggcgctga gatgcgcgct cagtgccgca
     3061 cctcgctcgg cggcgcgttc gcaaactccg cctcgaactg cgaaatctcc cgcgtcaagt
     3121 cttccaagat ccgctggcgc agcgcggcat cggagccgaa tgtcatctcc gcgacggtct
     3181 tggcaagcgc cgtgataatt tcgtcgacat cgatggtgtc tttttcaccg ctcaacgagc
     3241 catactcggc ataaaagtcg tcgatcacgt cgccgatgag gcagtgcaga cagggccgct
     3301 cgtcatccga ttccgtagca tccgacatgg ccaatcctcg catacgcaga tcaggtccgt
     3361 atccgccgtc gtccgggccg cgcctcgaat cgcgccggca ggggcggtct tggcggtccc
     3421 gagagaaccg cttcacttaa gtatgatcaa agtcttatgc ttgcaaacct ccgcccgggt
     3481 cgcctttgac ccgtaacggt tattttcagg cgctggcaaa ccccgaagat gacaacccgc
     3541 gctggccgcc ctatctaatt tggaccatgt tgcgtcccgc gtacctctcc caaaaactag
     3601 acggccccct accgacaaaa gacggtggca cgctgcgcac gatccgcgag gcgtgcgatt
     3661 acatgacggg tatgggtaag aagcgagagc tatgtcagca ctggcagcgc gcctgcaagc
     3721 tcattctcgc ccaagctgat gtagtgaccg ttagggcgct tgagctggcg ctgttctacg
     3781 acgccaagct tgacgttgcg ccgatggcct aggctagacc gcacaaagtt ggattcttct
     3841 gttccactcg cgcgccagtt tctacgctga gtgtcaatca ccttgtcggc gcccctctat
     3901 agccgggaaa cttacggtat agcttttttt caccgcctgg tgagcggcgt cgatgatttt
     3961 cacccacccc cgacgctgcg cacgaacgcc gtagcacggc cggaaattgc ggtcgcaagc
     4021 gcgcgttcgg agatgccgaa aaagacacca agcagcattg cgatagaacc tctttgcatg
     4081 aattcggccg cgttggtctt gagatcgccg atttgaatat tcatgacccc tgtccaaaac
     4141 agcaggcagg cagtaagcgt cagggcaatc acgaatataa tgcgcacgct gggatcgagc
     4201 agatcttcct ccagtatacc gaggtcgctg aatgacaaac tcacgcggcg gatagagaag
     4261 gagagccatg tcccgattgc tgctcccgcc gcagcaatca ggaaagctct gtggtcatac
     4321 caccagccac tcccgaccac tttggcgatg aaggcgtaaa gcaaaagcaa cggtaccgct
     4381 gcggtggcgg cggtggcacc caaaccacgc acataaatgt tcttgatgcg ccccgcctgt
     4441 tgcgcagtga attcgctgcg caattcattg agagcgagct tggccagcgt agtatgggga
     4501 ccttccagcc cgacaacggc gatgccggcg agcttcctca aataatcgtc gagcaaacgc
     4561 tgggccagct ggcgttccgc ttccgcccta cgctttcgtc gctgcttcgg cccgtaccac
     4621 catcgcttct cgattttttc cgcccgatca tccatgtgcc gagagacagt gctgataacc
     4681 gtatcggcat tgcctacctg cacgaataag tcttgcttct cctgggacgg ctgatcgtcg
     4741 gggggcggca acccaggcgt caccttgaat tcgagctcga ctacgcgttt aggttcgtcc
     4801 ttgtacttgg ggattacatt gaataagcgc gccttcagtt ggtctcgctt cccaatataa
     4861 attccctggc ttaccagcgc atggtcggca gctattctaa cagcgacgct gcctctgcgg
     4921 aattcctcga actccttccg aagactgtta gcggcctcct tgcggtaacc ctgccgtgcg
     4981 tcaaggcgcg ccaccgaatc tccggcagtt ttgtcggcga cttcgtcctc ggccagcctg
     5041 tcaagaatgt cagctatccg gtcgatatat tctttcactg gaccgtctgg cgcataattt
     5101 ttcgtgattg tgaagtcccc tgcgaagcgc agctctgcgg ccgctttgtc ctgagcaacg
     5161 agatcggggc agttctgagc gtatttctgt gcgacttcgg tcgtggcgct cgctgctgcg
     5221 tccgcgtcta tcctgccatc tgcgaattcc gtcgcgattt tgcgtagctc gcaaacctcg
     5281 gtttccctgt ggtcggtccg agcttgatga accgaggatc caaaaaaatg cgtgtcctgc
     5341 cgctcagcgt ccttaagcat cgtcagggta tctttgagct tgctgatata ggccgcctcg
     5401 ctgtccgttg ctttgtcggc tgggtcggtt tcgtaggttt cgattacgaa atcgttccgg
     5461 agacgaccag gaacactcca tgacgaaccg gaagcgggac caggtaggcc gatatccctc
     5521 acctcgaact ttggcatcac ttcttccgct tgttcgggca atcccgcaat ctcgcgtgcg
     5581 ttgcgtttca gtgtccgcgg agcaccggga ccgcgattga atgcggtatc gcgcaggaag
     5641 aattctacgg ccgggtgttg ataccatccg gcgacggggt tggtgtactc gataatgatc
     5701 tcccgagcac gcttttccgc atcgttgtag cgcccggcat gaatgagctt cgctaactct
     5761 gccgcctctt cgggatggta tccatcgttg atgccggcga cttcatagaa accgccgcgg
     5821 tcattccgtg gcaacggata aatcgccaga tgtcctttct tgtcgcgtcg cgcttcgaag
     5881 tcaaggataa gctgacccat atgctcgcgg tttgcttcgg tcgaatcgga cctggtgaca
     5941 atgagcggct cgaccggctg caccggggat ggcttcgctt tgttgaagaa aacctccaag
     6001 tcccacggct tggtgctgtc gtataagtct ttcgtggatt tgaccgatat gtgaatgtga
     6061 tgatcatgct tattgctgcc tttgtaactg cgccactgcc aagcaggatg cgcctgaccc
     6121 gaaccgctgg agatgcggcc gtttgaaata atatatttga tgcgcgggtc tctgatctgt
     6181 agcaaatagt tggcgaactt atagctatca aatccccctt tcggatcgtg cgtgatgtcc
     6241 atcgcggtta caacgccgac gccgcaatcc ttgacccacg gctcatgatc cgaattcggg
     6301 ttggcatcgt gatgccgctt gttagcaatc agtccatcca gatttttcgc gcgcttcggt
     6361 atcgcggcgt taacttgatc gcgcagtgta agcaacgatc ctgcagctct ccaagccata
     6421 gctaagcccc tttgggttat agacgctggc cggtcgagag aggcgtgcaa atccacccac
     6481 gcggctcgtg ctgctgcgcg cgcgccgcga gcggccacgc cacggccgcg ccgtcgaggg
     6541 tggctaagaa ttttcgtcgt tggatgtagc ttgccatgtg accctccggt tgggggtcat
     6601 tcatgcaatg acgcatcata ccacgcttcc atcgcgcggt caccagcggt aaggctcaga
     6661 accggttgcc ccggggcgga cctccgatct gggcgttaat ggagtacacg ctctggctaa
     6721 tcctcctgag aaccgtctcc acctgaagcc atcacgctgg cttcgacatc gctgcgttcc
     6781 aattccacat gctcagttgg ttgcaccgga gcaagagagc tttcaaggag gagctccaag
     6841 cggcctgcaa tgctatgccg aaggcacatc tgacctacat tgtcactgcc tacggcaagc
     6901 cacgatcgaa gtacggcctc ggcaatgact ttgcggagat gggctaccga ggccggcttg
     6961 cccgcgcgct gtcgaatgca cggcctcaag aagggcggaa tgcgtcgcct cgccgagcat
     7021 ggcaacacca ctcacgaact gatggcgatc tccggtcacc gtacgctaag cgaggtgcag
     7081 cgctacaccg atgacgcaga ccgcacgcgg cttgctacta gcggaatggc gaagcgtaga
     7141 gaccgaactg agaacaccga agtggcaaac ctcgatcccc ggagtggcaa acctgcgtct
     7201 aagtccttga gataaaagaa tctgaaattg ttattggcgg tcccgagagg actcgaacct
     7261 ccgaccttcg gtttaggaaa ccgctgctct atccaactga gctacgggac cacttaagtc
     7321 ttttacttgc gcaggtagcg tttcgtcctc gcctcacaat agccttctgc ggcggcatag
     7381 caggccgtgt agcatagcat agccgcttgc ggctccgcac ggcacccatt tgacggtctc
     7441 ccggaccacg catgacggcg acttgtcgcg accagggagc gacgcggagc tcgaccacca
     7501 gcgattcatg cctgaccgga accaagcccg cattctgcac gttccactac aacaatcgca
     7561 cgagtagcag cgtcatgccg cttacgcgcc gccactttat cggcactgca gcgagcgcag
     7621 ccgcgctcgc atgtcaatgc gttgaggcgc aagaggaaca cgcccttttc tggcgcatcg
     7681 aggttcctga cggcggccgg ggcgtcgcgt tcggatatac gcgcgtcgcc gcggctgttg
     7741 ttcccgaggt tgtgaaggac ggcgttcggc tcatggaagg cgcaaagcgt gtcgtactgg
     7801 acatgaacaa tacccgcttc cccgcgatga agattgatcc gaacctgccg tcattgtggc
     7861 cgaagctcga tccggcgcgc gccgaacaag tccgcagcat cctcacctcg atgcacgttc
     7921 cgcccgccca gatcgaaaac ctgccggggt tcttcatcgc cagccttctc tacgcggagg
     7981 gtcaggccaa cccggtgccc tcggtgggag gcgtcatcgt cgaccaggcg aaggcgctcg
     8041 gccgtccgat gacggttttg ctcgaggcat ccgacgtcga gca
//
LOCUS       DATLJT010000188         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3225424,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000188 DATLJT010000000
VERSION     DATLJT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225424"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..666
                     /locus_tag="VM910_04265"
     CDS             193..666
                     /locus_tag="VM910_04265"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HVI61796.1"
                     /translation="MPAPKNPDWLNSVHIEGISLADLARTLPAMLGTANAERLNFIVR
                     PFGADVTLMQLDDLDREKLARVAPAMFLSIETSPGNFQAWLAMAGEEDKDFTRRVKRG
                     TDADINASGAPRIAGSLNLKPAYAPNHPRVAIRQIQLGRKTDARRARAAWRGRGA"
     gene            761..997
                     /locus_tag="VM910_04270"
     CDS             761..997
                     /locus_tag="VM910_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61797.1"
                     /translation="MNNAGTDKDYSKADIGWCITAIRRKFGINETAAMLLEVSEHARH
                     PSNGKRYAERTAARAADYVQQRTRPRLPIKQDHG"
     gene            1261..1632
                     /locus_tag="VM910_04275"
     CDS             1261..1632
                     /locus_tag="VM910_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HVI61798.1"
                     /translation="MMIGKFTAEGEGYQGWIEALALRVEGVRITPMLAKQGAGPDYVV
                     SAYTDGEEPSDIDIYELGAAWKKTSKKGKPYLSVKLDGPTLAAPINCALTQQQDGSYG
                     LIWNRKDKDEEHSAADEQAAA"
     gene            complement(1698..2114)
                     /locus_tag="VM910_04280"
     CDS             complement(1698..2114)
                     /locus_tag="VM910_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61799.1"
                     /translation="MSKSFSQAPKPQQLTAEVISAFERRGAGQDTRARIPSNAENRDP
                     TNVGDVAPELAGPDLQPTDVERGEPTKVETVKASKLGLGIPTKEGTAERTRRLSIDLP
                     ESWHRRFKIACTRADKKMIEEVTEFIKRRTLELEKE"
     gene            complement(2065..2742)
                     /gene="parA"
                     /locus_tag="VM910_04285"
     CDS             complement(2065..2742)
                     /gene="parA"
                     /locus_tag="VM910_04285"
                     /inference="COORDINATES: protein motif:HMM:NF041546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HVI61800.1"
                     /translation="MIWGFVNQKGGVGKSTLSQNVADCLSRLGQKVLLIDADKQGTSS
                     DWASLRAENRFTVVSMARENMALDAIKIAADYDFTLIDGPPHADTISRSCIVASDLVV
                     VPIEPGGASRWSSNLTVRQLQEAQALKPTFKCGFVVSRKIQGTVLGRDARAMAADAGI
                     PIFDTEIEQRIAYAEALTMGKTIFEWGTNNAAVREIQALTHELLDALKPDEQVVFPGA
                     EAAAANG"
     gene            complement(2822..3277)
                     /locus_tag="VM910_04290"
     CDS             complement(2822..3277)
                     /locus_tag="VM910_04290"
                     /inference="COORDINATES: protein motif:HMM:NF023398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI61801.1"
                     /translation="MNLAGVKDRNPDEMFFAACLWQEKNPRALIPLPFWSAPEQRFRP
                     LVELQFGPAWVAEFLECVAAAAMVGLHELNRLQEAEKKGRMMGATARSRLPKAVDAVL
                     RAHIVTAAGLAKSIRVTPQAALSLLQQLAAAGIVREVTGRASWRAYALI"
     gene            complement(3410..3886)
                     /locus_tag="VM910_04295"
     CDS             complement(3410..3886)
                     /locus_tag="VM910_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61802.1"
                     /translation="MFLPPWETEDEESLEPPGPPRARKQPSEPDYAHPLLIPLVRAQN
                     AVARLQAKTEVASDVVAEGLRARMSYLEAAGWLNYTHFSIHPWDLALRDHGLTTSNGA
                     ADHADRLAAVLPSTTAQEGELQVAPSDLIVNGALSYARLWRRLAEFRTWRPLADVE"
     gene            complement(4995..>5238)
                     /locus_tag="VM910_04300"
     CDS             complement(4995..>5238)
                     /locus_tag="VM910_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61803.1"
                     /translation="DATPHGFRTTFTNWAYASRSFRDIAIELSLDHAYGNQVHRAYRD
                     ERLVEERRELLQAWSDYCNGRTADIIRLPTPVTRTA"
BASE COUNT         1071 a         1564 c         1579 g         1024 t
ORIGIN      
        1 ccgggttggc gtcgcacacc aggtgcagtc gcgtggctga caaaactcgt cgccaccttg
       61 acgccgaaac ggcggacacg tccgactatg cactgatccc gcttagcgtg cagttttgaa
      121 caaatcgtgt agcgacccca tccccgccgg ccgcgtacac tgaggagagc actgcagtcg
      181 ctcagcggca aaatgcctgc cccgaaaaat cccgactggc tcaactctgt tcacatcgag
      241 ggcatcagcc tggccgatct cgcccgcacg ctgccggcga tgctcggcac ggcgaacgcc
      301 gaacggctga attttatcgt gcggcctttc ggcgccgacg tgacgttgat gcagctcgac
      361 gatctcgacc gtgaaaagct cgcccgtgta gcccccgcca tgttcctcag catcgaaacc
      421 tcgccgggga attttcaggc gtggctggca atggccggtg aggaagacaa ggactttacc
      481 cgccgcgtga agcgcggcac tgacgccgac ataaacgcga gcggcgcgcc gcgcatcgcc
      541 ggcagcctca atctgaaacc agcctatgcc cccaatcatc cacgcgtggc gatccgtcag
      601 attcagctcg gccgcaagac cgacgcccgg cgagctcgcg cagcttggcg tggtcgcggc
      661 gcctgaggaa tttgcgccgt tatcggagcc ccctacccgc ttcgagatcc gtggcgatca
      721 caaatggccg aggtacacgg tagcggttga cctggcgccc atgaacaacg ccggcaccga
      781 caaggactac agcaaagccg acatcggctg gtgcatcacg gcgatcaggc gaaaattcgg
      841 catcaacgaa acggctgcga tgctgctcga agtcagcgaa catgcgcgcc atcccagcaa
      901 tgggaagcgt tatgccgagc gcaccgccgc cagagccgcc gactatgttc aacaacgcac
      961 ccggccccgg ttgccgataa aacaggatca cggttaggaa catgggcggg gcaatagctg
     1021 ggggataatt gaccattgcc ctcgttcaga agaagttgca cagtcaatcg caagaatgac
     1081 gagacataaa gcagtgcgcg ttcaatagcg ttcaattcgt ccgaaagatt acacagcaaa
     1141 ttgtgacgtg aaggtgccgt tgcgtttcaa cgcagcggcg ctgcggttta tgcggctgct
     1201 tgtgacaatc gtcagcgagg ccgtcaatcc cggcggcttc tcaaccgaag gaggcgcaca
     1261 atgatgatcg gaaaattcac ggcggaaggc gaaggctatc aagggtggat cgaagcgctg
     1321 gcgctaaggg tcgaaggagt ccgcatcacg ccgatgctag cgaagcaggg agccgggccc
     1381 gattacgtcg tgagcgcata caccgacggc gaggagccaa gtgacattga catttacgag
     1441 ctcggcgcgg cgtggaaaaa aaccagcaag aagggcaaac cgtatctgtc ggtgaagctc
     1501 gacggtccga cgttggcggc cccgatcaat tgcgccctca cccagcaaca ggacggctca
     1561 tacggcctta tctggaaccg aaaggacaaa gacgaagaac acagcgctgc cgacgaacaa
     1621 gccgctgcgt aagagcggaa acgaccccgc cggcatcacc gacgacgaac acggacgaca
     1681 ctgatctcgg cggggcgtta ctctttctcg agctcgagcg tgcgacgctt gatgaattcc
     1741 gtaacttctt cgatcatttt tttgtcggcc ctggtgcaag cgattttgaa gcgacgatgc
     1801 cagctctccg gcagatcgat gctgagacgg cgggttcgct cggctgtgcc ctcttttgtg
     1861 ggaatcccca atccaagttt tgaggctttc accgtttcca cctttgtggg ttcccccctt
     1921 tccacatcgg tgggttgaag atcaggccca gctaattcag gagcaacgtc acccacattt
     1981 gtggggtccc tgttttccgc atttgaggga atgcgggcac gggtatcctg accggcgccg
     2041 cggcgctcaa aggcggatat gacctcagcc gttagctgct gcggcttcgg cgcctgggaa
     2101 aacgacttgc tcatcgggtt tcaacgcatc cagcagttcg tgggttaggg cctgaatttc
     2161 gcgcacggca gcgttgttgg tgccccattc aaagatcgtc ttgcccatgg tcagagcttc
     2221 ggcataggcg atgcgttgct cgatctcggt atcgaagatc ggaatgccgg cgtcggccgc
     2281 catggcgcgc gcatcacggc cgagaacggt gccttggatt ttgcgggaaa ccacaaaccc
     2341 acatttgaag gttggtttaa gtgcttgagc ttcctggagt tgtctaacgg tcaggtttga
     2401 cgaccaacgg gaagcgccgc ctggctcgat cggcacgacc acgagatccg aggccacaat
     2461 gcaggagcgt gaaatcgtat cggcgtgcgg cggtccgtcg atcagcgtga aatcgtagtc
     2521 ggctgcgatc tttatggcgt cgagggccat gttctcccgc gccatgctca cgacggtaaa
     2581 gcggttttcg gcccgcaagc ttgcccagtc ggacgacgtg ccttgtttat cggcgtcgat
     2641 cagcaagacc ttttggccga gccggctcag acagtcggcg acattttggg aaagcgtgga
     2701 tttgccgacg ccgccctttt gattgacgaa cccccaaatc attgcacaac attagttgat
     2761 tcgtgggttt gtgcaaatgt acaaagaagc gaatgtgggt atgtggtaaa ccacaatcct
     2821 tttaaatcaa ggcgtaagcc cgccacgatg cgcggccggt gacttcccga actatgccgg
     2881 cggccgccaa ttgctgcagc agactgagcg ccgcttgcgg cgtgacgcga atcgatttcg
     2941 ctaaaccggc ggcggtgacg atatgggcgc gcagcaccgc atcgaccgct ttcggcagcc
     3001 gggagcgtgc agtggcgccc atcattcgac cctttttctc agcctcctgc agccgattga
     3061 gctcgtgcag cccgaccatg gctgcagccg cgacgcattc gagaaattct gccacccaag
     3121 ccggcccgaa ttgcagctcg acgagggggc gaaaacgctg ctccggcgcc gaccagaagg
     3181 ggagggggat cagcgctcga gggttttttt cctgccatag gcatgcggcg aagaacatct
     3241 cgtcgggatt gcgatctttg acgccggcca ggttcatcca actgcgcgca acgcgacccg
     3301 cctgaattaa gtctggtgcc cgctccagca tgcgaattgc cgtcagccag tcggcgattt
     3361 cggcgtcttc gatccccgaa aagcccaatg ttttcagggt ttcgcgcagc tactcgacat
     3421 cagccaaagg ccgccaagtg cgaaattccg ccaggcgccg ccacaaccgc gcgtaactca
     3481 gggcgccgtt gacgatgaga tccgaaggcg caacctgcaa ttcgccttcc tgggcggttg
     3541 tcgacggcaa tacggcggcg agccgatctg catgatccgc ggcgccgttg gaggtggtga
     3601 ggccatggtc tcgaagcgcc agatcccacg gatgaatgct gaaatgcgtg taattcagcc
     3661 atcccgcggc ctcaagatac gacatccgcg cgcgcaggcc ttcagccaca acatccgacg
     3721 ccacctcggt tttcgcttgc agccgcgcca cggcgttctg tgcccggaca aggggtatca
     3781 gcagaggatg agcataatcg ggttctgacg gctgttttcg cgcacgtgga gggcccggcg
     3841 gttcgaggct ttcttcgtcc tcggtttccc acggcggcag gaacaagttt tcatcgtctt
     3901 tttggggctc tcccggcata tcgactgcga aatcactaag atgctgaaat tgctcatatc
     3961 gtgaccatgt tttgaaagca aatcgaggct tttctttggt cgtcgcgaca tcggaaatgc
     4021 gcctgatcga atgccttcac atccgatcgt cgaagatgtt tggaatccac acgagatcgt
     4081 tagaaggagc aggcccaaca cttgcgccgg cagggtccat ctggaagctc cgatcacctg
     4141 gtgcattctc acgaataagt ggtgaggata tgccctgccg agacctgctc cggttttgct
     4201 aaggccctgt gtcagcgtgg gtttttggca cgttctcctc caaggcccgg ccgagctctc
     4261 atgatcttct gttttcgaca cgaggccgag gagcggatca aagccatgct cgcacagccg
     4321 cgtatcacga gcccggcgac gctccacgac aagcgctgat cattcggccg gcgatcgtgt
     4381 gcccgccatt cgtctcgacc gagggacggg catttccggg ccactcgcga gcggctatct
     4441 ccatggcacc gtcgccggaa agcaaggccg gctgtagctc gaatgcacgg tgtgggcctt
     4501 gcgccaagct ccggagcgga tgaccggcag ggcaggggag ggagaccgct ttgttccgtt
     4561 atggtgtgca tcgcgtcgtg caaattgccg tcatgacgtc cgttcgctgg agatcccgga
     4621 gcagggtcct caatttgcgt gctccctacg atccactccg gcgcttcggg ggttcggccc
     4681 aagggccttc cgtccaagga ccgtgccggc ggtctccccg actttcagtc gctgcgcccc
     4741 ccacaagccg ggtgatcccc ctccgccgtc acgccccgcc gctgccaaag cctcggaccg
     4801 tatccggtcg ctatcgaggc cccaattccg gggcttcagt aacgaaagga gaaagtcatg
     4861 attatcggta acttcaccta cagcaaaagc ccaggacact tacacgggcg aggttgcgac
     4921 cacgtagagc tgcgaccggg agataccggt gcgacgggaa aactcgccga cggtcgaggt
     4981 gatgatgtcg ctgctcatgc tgtcctcgtc accggggtcg gcagcctgat gatatcggcg
     5041 gtccggccgt tacagtagtc gctccaggcc tgcaggagtt cgcggcgttc ctcgacgagg
     5101 cgctcgtcgc gataggcgcg gtggacttgg ttgccatagg cgtgatcgag cgataattcg
     5161 atggcgatgt cgcggaagct gcgggatgcg taggcccaat tagtaaaagt cgttctgaag
     5221 ccgtgcgggg tggcgtcg
//
LOCUS       DATLJT010000189        12712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2076805,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000189 DATLJT010000000
VERSION     DATLJT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076805"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VM910_04305"
     CDS             <1..813
                     /locus_tag="VM910_04305"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HVI61804.1"
                     /translation="LAGLFSALRVKGGKLSDQTLLFLGAGEAATGIADLVVSAMMAEG
                     TSEAEALRRNWLVDSRGLVVKGRPGLSGHKLRFAHEQAPINDFLTAIKTLKPTAIIGV
                     AAVGGAFTPEVLQTMARLNEQPIVFALSNPTSKSECSAEHAYRHTGGRALFACGSPFD
                     PVKLDGKTFVPRQGNNSYIFPGVGLGVIASGSRLVTAEMFMAAAHTLANSVSEGDLKQ
                     GSLYPSLPRIREVSAQIGAAVANVAYQRGLAAGPPPNDLIGFIQSQMYDAHY"
     gene            complement(834..2291)
                     /locus_tag="VM910_04310"
     CDS             complement(834..2291)
                     /locus_tag="VM910_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI61805.1"
                     /translation="MRSRRNILGMMAATAAAVGSSPVRVFAQTAGSNPGKVMNVLTDY
                     MSAARARALPDEVIEHAKYHLLDTIAAMISGSELPPGQAAQRYVREHSGNGTTTIVGA
                     SLTAPPIDAALANGVMAHADETDDSHNESRSHPGCAVVPAALAAGEVYGIDGNVLLRA
                     VTLGYDVGPRVVLAMGGAAFSYESSLSTHSIAGTFGASAAAACAAGLDARQMRWALDY
                     TAQQSSGIVAWRRDTDHIEKAFVFGGMPARNGVTAALLVKSGWNGVDDIFSGPDNFFA
                     AYSPKAQPEKLIEKLGERYEITQTDIKKWSVGSPIQGPLDAIEAIREKKPFEADQVKK
                     VTVRLAPSVATVVDNRDIPDICLQHLVAVMLIDKTVSFHAAHDKPRMQDAAVQKQRAK
                     VNLVRDEELAKLLPLRETVVEIEFNDGKRLSERVSAVRGTPRNPMGRDEVIDKARDLI
                     TPVLGRESCTRLIETVLEIEKLTEIRSLRRLLQRG"
     gene            2425..3312
                     /locus_tag="VM910_04315"
     CDS             2425..3312
                     /locus_tag="VM910_04315"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI61806.1"
                     /translation="MEIIPLGPGFGVELRGVTLADVASDDAVYAAVRSAFEEHSVLVL
                     RGQQVDDELQLAFSRRFGPPEVTKIGSQGAGTHFVVLSTIGPDGKVVPADHRMQMRNK
                     ANQLWHTDSSFKSTPALTSILSARIIPDHGGETEYVSTRLAFERLDASLQRRLENSFA
                     WHHYAHSRGKIAADLASPEELAALPPQCWRLIWRNPVNGRGALYLASHAYAVEGMEAR
                     AGRELIEELTAAATAPGKSYLHRWRQGDVVMWDNRATMHRGRPWPSHEPRLMIRTTIS
                     ATEIDGLATMRPPSWQAAE"
     gene            3587..4603
                     /locus_tag="VM910_04320"
     CDS             3587..4603
                     /locus_tag="VM910_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61807.1"
                     /translation="MNAKTASWLTAHGRLWYGDRWYRLAWIVWPQALGLMLFVTLWIG
                     HPAGQGFIPWAKPLLEAPKAPPAAAPLNNPPNQQVVQPPAVTDVLAPCKSDDHAQAIR
                     DCTALLASGNLQGDNIPYAYYHRGWAYYQTSQYQVAMADYNRAISMQPRAPEFFLDRG
                     ALLIDLKNNERALQDLDYAILLKSDYALAYIDRGIALHNLNRQSEALVSFNKAIELDP
                     SQFSAFENRASIYEDISNWRGVYDDGNKMIELNPNNRLGYEYRGHAYLEVGQQEAAIR
                     DFNRAISIDPSAIYGWRMRGRAYYFLNQFDNAMADFQAALRIDPKDSGTIDFINDLKR
                     KRGR"
     gene            4848..5417
                     /locus_tag="VM910_04325"
     CDS             4848..5417
                     /locus_tag="VM910_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61808.1"
                     /translation="MSDSAQTPASSGSPSTSKPVTPIKASMSPTIVGAVGVLLVLAAA
                     GAVAMKSGVGLGIGDSTRTTVSFVDPRDLNDAAGTLTQSVAGGLVDDARRCRIPLVSM
                     TIAKGTAQVGSTLRIRSGSYVSPYFTVTEGMQRIAVPYPAPYGSGAGTFVVEGNATGA
                     ILGLTPTKVLLDLPTAQSIPVWWRPVSPC"
     gene            5431..6549
                     /locus_tag="VM910_04330"
     CDS             5431..6549
                     /locus_tag="VM910_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI61809.1"
                     /translation="MKNLFKDQQILAIIVGSVAAAIITAWVMNPQSGPAGWIKGLPPA
                     AQHTLSGIAIAFVIASVGLLVSSIAGGYARTGALQPASSGGMSGAFKMLFSPEREIPD
                     TPPNKAIEDLEAMVGLVPVKREVNGLIARMQVEQKRREQGLPTAPLSLHMVFAGPPGV
                     GKTEVARALGDIYRALRVLRVGHLIEVQRSDVVAGYIGQTAPKMLEKCNQALDGILFI
                     DEAYTLAANAGLGADFGKEAIDTLLKFMEDHRDRIMVIVAGYPNEMRRFIDTNPGLAG
                     RFTKTIDFPRYNADDLCEILRRMAAKQHFSLPENFPTRIKPWIAERSRAEDWANAREM
                     RTLLEKAREAQAMRIATEPSADLSRIEIVDLMKAIGRA"
     gene            6557..9172
                     /locus_tag="VM910_04335"
     CDS             6557..9172
                     /locus_tag="VM910_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI61810.1"
                     /translation="MMITWLTIAFILVAGFSMTFIAMRLVEQHALPAVYSPDLVRPIR
                     IVEAEERHEPSAMEVALAKLDAMVGLAPVKQEVKALIARMQVEQKRRDQGLDVSALSQ
                     HMVFTGPPGVGKTEIARLIGEIYQGLKVLRKGHVVETDRASLVAGYLGQTAIKTLDKC
                     REALDGILFIDEAYSLAPGLGADFGKEAVDTILKFMEDNRDRIVVIVAGYRNEMRRFI
                     DSNPGLASRFSKTIDFPSYTTAELCEIFKRMATRQQFNLPEGFEARLKPWIESRAKAE
                     DWANAREMRTLLERSREAQAMRVASDPSADISVVTIQDIVLATGQQDEGNEAAIAAAL
                     DKLNAMVGLAPLKEEVQALIARKQVEQKRREQGLDVSSVSQHMVFTGPPGVGKTEVAR
                     VIGEVFRALKVLRKGHVVETDRASLVAGYVGQTAIKTLDKCKEALDGILFIDEAYSLA
                     ATPGLGTDLGKEAIDTLLKFMEDNRDRIIVIVAGYRNEMRHFIDSNPGLASRFTKTFD
                     FPSYSPAELFEIFKRMAARQQFNLPQDFEAKLKPWIESRAKAQDWANAREMRTLLEKT
                     REAQALRVAKDASADVSVVTIEDVVHATGQKIEDNEASVAAALAKLDEMVGLAPVKQE
                     VKRLSARMGVEQKRKEQGLPVAAVSQHMVFTGPPGVGKTEVARVIGEVFRSLKVLRKG
                     HLVETDRAGLVAGYIGQTATKTLDKCKEALDGILFIDEAYTLAAIQGGAHDFGKEAID
                     TLLKFMEDNRERIVVIVAGYPNEMRRFIESNPGLSSRFTKTIDFPPYGVVDLCEILKR
                     MAARQKFSLPDDFQAALTPWIEQRSKVQDWGNARSMRTLLEKARESQAIRISTDADAD
                     LSRIETADLLDATVS"
     gene            9201..10205
                     /locus_tag="VM910_04340"
     CDS             9201..10205
                     /locus_tag="VM910_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61811.1"
                     /translation="MNSAYSTWLSAHGRLWYDDKLYRAAWIAWPQALAAVMVLLFWAM
                     PPSNRNVPWAKPVDANVRYNALRTLRDSAKSDRQAMETLERDAVAGEMNAQFFYATLF
                     DPDLKLSTIVQPDVTKAVDWYGKAASQGDEYSIGNLALTYARGLYIRQDYTRSCFYAR
                     KLANSIAFANALNLKGECYSRGLGGTPVDLDQAAAAYESAATKGHLRATAALGYFYEN
                     GLGGKPRNYEMALKYYRFAADKGDSLGLHNLGAAYNSGLLGLQRDGSEAARLILHALE
                     NKYDVTVQSLTTRPDLWSPDFWQNLQRRLGEKGFYSGVIDGRANAATLDAVKRLGGRG
                     "
     gene            10318..10887
                     /locus_tag="VM910_04345"
     CDS             10318..10887
                     /locus_tag="VM910_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HVI61812.1"
                     /translation="MSTRIAIAVALIVIDPSGSFVLAQTSLGGPEQLPPSIAPTPPPL
                     SPPANPPASSPPRMDYIPPMIPPKPAPNQPSTTADFYWGAIAFTADGSYSSVWKKSSQ
                     PEAEAYVLRECARFGRGGCEVTTFSGQECVGLATFIGNYRRRRWNLSFTAGGSTYPEA
                     QGAALERCNSDERSHGQCQPRVAACADGR"
     gene            complement(10895..11899)
                     /locus_tag="VM910_04350"
     CDS             complement(10895..11899)
                     /locus_tag="VM910_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61813.1"
                     /translation="MRRREFIGLAGAAAAASIGPRSLRAQQKQHRLAFVHSGIPADKL
                     TASAGPFWVRQVYETLAGLGDVEGSNLIVERFSAEGRSERFATLAAEVVGRNPDVVIV
                     NLNDFVKAFMTATSTIPLVAVMGDPVAAGFISNIARPGGNLTGVAINAGIEIYGKRLQ
                     IVQEAMPKVRKVANLLSGVWTVSPGEAGYQEAGRRLGMEMVAKEMVEVNETQLRRMFE
                     EMAEQKFDAAVVDEGGSFLAQRALLTKLAEEHRLPVIYPYRDYVELGGLMAYAPDLGE
                     LAQRIAGDVHQIFNGAKAGDIPFYQPSKFQLIVNAKAAKAIGLELPQALLAGADEVIE
                     "
     gene            12155..>12712
                     /locus_tag="VM910_04355"
     CDS             12155..>12712
                     /locus_tag="VM910_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI61814.1"
                     /translation="MTRSSKIAKIELLACDAGWRNYHFVKATTDDGAVGWSEYDEGFG
                     SPGVSAAIERLSTRVIGQNAFQHERVYAELFAATRPATGGVVAQALGAIENALLDAKA
                     KLLGVPCYELLGGKVRDSVRVYWSHCATWRINHPAWYKPPIESLDGVKAIGREVREKK
                     FTALKTNIFTYEGGKPTGWRPGFGSP"
BASE COUNT         2544 a         3956 c         3849 g         2363 t
ORIGIN      
        1 ctggctggct tgttctctgc gctgcgcgtc aaaggcggca agctcagtga ccagacgttg
       61 ctgtttctcg gagctggcga agccgcgacg ggcatcgccg accttgtggt ttcggccatg
      121 atggccgagg ggacatccga agccgaagcg ctaaggcgca actggcttgt ggattcccgt
      181 ggcctggtgg tgaagggccg gccgggattg agcggtcaca agctccgatt tgcgcatgaa
      241 caagcgccga tcaacgattt cctgacggcc atcaagacgc tcaagcccac ggccattatc
      301 ggtgttgccg ccgtcggcgg ggcctttacc cccgaggtgt tgcagaccat ggcacggctc
      361 aacgaacagc cgatcgtgtt cgcgctctcc aatccaacct cgaagtccga atgttcggcc
      421 gaacacgctt atcggcacac gggcggacgg gcgctgttcg cctgcggcag cccgttcgat
      481 ccggtcaagc ttgatggcaa aaccttcgtg ccccgacagg gcaacaacag ctatatcttc
      541 cccggcgtcg gcctcggcgt gatcgccagc ggcagccggc tggtgaccgc cgagatgttc
      601 atggcggcgg cgcacacgct cgccaattcc gtgagcgaag gcgatctcaa acagggcagc
      661 ctttatccgt ctctgccccg gatccgtgag gtttcggcac agatcggcgc tgccgttgcc
      721 aatgtcgcct atcagcgagg gctcgccgcc ggcccgccgc ccaacgacct catcggcttc
      781 attcagtcgc aaatgtacga tgcgcactat tgaggtagcg cgaggcaaat caatcaaccg
      841 cgctgaagca ggcgccgcag gctgcgaatc tcggtcaatt tttcgatttc gaggaccgtt
      901 tcgataaggc gcgtgcacga ttcacgcccg agcacgggcg tgatcaagtc acgagccttg
      961 tcgatgactt cgtcgcggcc catcgggttg cgcggcgtgc cacgcaccgc cgagacacgt
     1021 tctgacaggc gcttgccatc attgaactcg atctcaacca cggtttcgcg cagcggcaag
     1081 agtttcgcca actcctcgtc gcgcaccaga ttgactttgg cgcgctgttt ctgaacggcc
     1141 gcgtcttgca tgcgcggctt gtcgtgcgcg gcatgaaacg agacggtctt gtcgatcagc
     1201 atcacggcga ccaagtgctg caggcaaata tccggtatgt cgcgattgtc caccacggtc
     1261 gcgaccgacg gcgcaagccg cactgtgact ttctttacct gatcggcctc gaacggcttc
     1321 ttctctcgga tcgcctcgat cgcatccagc ggaccctgaa tgggtgagcc gaccgaccat
     1381 ttcttgatat cggtctgggt gatctcatag cgttcgccaa gcttctcgat cagcttctcg
     1441 ggttgtgcct tcggtgagta ggcggcaaag aaattgtccg gacccgaaaa aatatcatcg
     1501 acgccgttcc agccggattt gacaagcagt gcggcggtca cgccatttcg cgcgggcatg
     1561 cctccgaaga cgaaggcctt ctcgatatgg tcggtgtcgc gacgccaggc gacgatgccc
     1621 gaggactgct gcgcggtgta gtcaagggcc cagcgcattt gccgcgcatc gagcccggcg
     1681 gcacaggcgg cggctgctga agcgccgaac gtcccggcaa tgctgtgagt ggaaaggctg
     1741 ctttcgtagc tgaaggcggc gccgcccatc gccaacacca cgcgcggacc gacatcatag
     1801 ccaagcgtca cggcgcgaag aaggacattc ccgtcgatgc catacacctc gcctgccgcc
     1861 agtgcggcag gcacgaccgc gcagccgggg tgagaacggg actcgttgtg cgagtcatcc
     1921 gtctcatcag cgtgagccat caccccattc gcgagcgccg catcgatcgg cggcgcggtg
     1981 agcgaggcgc ccacgatggt cgttgtgccg ttgccgctgt gttcgcgaac ataacgctgc
     2041 gccgcctggc caggaggcaa ttccgacccc gatatcatcg cagcgatcgt atcgagcaga
     2101 tgatacttgg catgctcgat tacctcgtcg ggcaacgcgc gtgcgcgcgc ggcactcata
     2161 taatcggtga gcacattcat cacttttcca gggttggaac cagcggtctg cgcgaagacg
     2221 cggacaggcg acgagccgac cgctgccgcc gtcgccgcca tcatcccaag aatgttgcgt
     2281 cgcgatcgca tggggacact catcgaataa gtgagggccg aagtccatag cctattcgtc
     2341 aaaaagcctc gcggcgaccg cgattgccgg gagaacgggc ttgccgtaag cttaagtcaa
     2401 gaaaccagct cgaggaattg tccgatggag atcatcccgc tgggtccggg attcggcgtc
     2461 gagctgcgcg gcgtgacact ggcagatgtt gcttccgacg atgccgtcta tgcggcagta
     2521 cgttccgcgt tcgaggagca ctccgttctg gttctgcgcg gccagcaggt ggacgacgag
     2581 ctacagcttg cattctcgcg ccgcttcggg ccgccggaag tgacgaaaat cggctcacag
     2641 ggcgccggca cccatttcgt ggttctgagc acgatcggtc ccgacggcaa ggtggtgcct
     2701 gctgatcatc gcatgcagat gcgcaacaaa gccaaccagc tctggcatac cgattcatcg
     2761 ttcaagagca ctcctgccct cacctcgata ctttccgcgc gcatcattcc agatcatggt
     2821 ggagaaacgg aatacgtttc gacccgcctc gccttcgagc ggctcgatgc gagcctgcag
     2881 cgacggctgg aaaactcttt cgcctggcac cactacgcgc attcgcgcgg gaagatcgcc
     2941 gccgatctgg caagcccgga agaacttgcg gcgctgccgc cgcaatgctg gcgcttgatc
     3001 tggcgaaacc ccgtcaacgg acgcggggcg ctctatctcg cctcccacgc ctacgcggtc
     3061 gaggggatgg aggcgagagc cggcagggag ttgatcgaag agctgacggc cgcggcgacc
     3121 gcgccgggca agagctattt gcaccgatgg cgccaaggcg acgtcgtgat gtgggacaat
     3181 cgcgccacta tgcatcgcgg taggccgtgg ccatcgcacg aaccgcggct catgatccgc
     3241 accacgatct cggcgacgga aatcgacggt cttgccacca tgcgtccgcc atcatggcag
     3301 gcagcagaat aagactggct caaattatgt gacctcacgc actttcacag agtgctgcag
     3361 cggcgaatat cgatccgaga gtggagcgtc tggcaaccgg ctcctcctcc tggggccacc
     3421 gcatgagccg gcatccgcgc aacctgtcgg gcaagactac cgcattccag ggcaggtcca
     3481 cctgtgccgg cgagctgttg aacggttgcg gccgccgttt tattttggcc atcataccgg
     3541 ctcccgagct cgatttcatt ccagccttct ccagaaaggc agcgcgatga acgcgaagac
     3601 cgcgtcctgg ctcacggcgc acggcaggct ctggtacgga gaccgctggt accggcttgc
     3661 ctggatcgtc tggccgcagg cactcggcct gatgctgttc gtaacgctat ggatcggtca
     3721 tcctgccgga cagggcttca ttccctgggc gaagcccctg ctcgaagcgc cgaaggcacc
     3781 tcccgccgct gcgccgctca acaaccctcc caaccagcag gttgtacagc caccggcggt
     3841 gactgacgtc ctggcgcctt gcaaatccga cgatcacgca caggcgattc gcgattgcac
     3901 tgccctgttg gcttcgggaa acctccaggg cgacaacatt ccatacgcct attaccatcg
     3961 tggctgggcc tattatcaga ccagccaata tcaggtagcg atggccgact acaatcgcgc
     4021 gatctcgatg cagcctcgcg ctcccgaatt tttcctcgac cgaggcgcgc tgctgatcga
     4081 tctcaagaac aacgaacgcg cgctgcagga tctcgattat gccatcctgc ttaaatcgga
     4141 ttacgcgctc gcctatatcg atcgcggcat agcgctgcac aatttgaacc gccaaagcga
     4201 ggcgctggtt tctttcaaca aggccatcga acttgatccg tcgcagttct cggctttcga
     4261 gaaccgcgcc tcgatctatg aggacatttc gaattggcgt ggcgtctatg atgacggcaa
     4321 caagatgatc gagctcaatc cgaacaatcg gctcggctac gaatatcgcg gtcacgccta
     4381 tctcgaagtc ggccagcagg aggcggcgat cagggatttt aacagggcca tctccatcga
     4441 tcccagcgcg atctatggct ggcgcatgcg gggccgtgcc tactactttc tcaaccagtt
     4501 cgataacgcc atggcggatt tccaggcggc gctgcgcatc gatccgaagg acagcggtac
     4561 tatcgacttc atcaacgacc tgaagcgcaa gcgcggtcgg taacgacacg ctccttcctg
     4621 cacgcttgag ttgtcggcag cgcactgctg cctgctcaag tcgatccttc agcaccaact
     4681 tgcatcgtgc gaccggaggt tcaaacggaa taccgtcaga ctgtgcgccg tgtcacagtg
     4741 caatgacggg cgcgctggca gattgggagt cggaatagct tttgcgtcgc cttttccgct
     4801 tgggtaagct tctcgggcga atggggctgc taaacagggg cagggcaatg tccgattcag
     4861 cgcagacgcc ggcgagcagt ggctcgccgt caaccagcaa acccgtcact cccatcaagg
     4921 ccagtatgag ccccacgatt gtgggcgcgg tcggcgtgct gcttgtgctg gccgccgccg
     4981 gcgcggtggc gatgaaatcc ggcgtcggcc tcggcattgg cgattcaacg cggaccaccg
     5041 tcagcttcgt cgatccgcgc gacctcaacg atgccgcagg cacgctcacc caatccgttg
     5101 ccggcggcct ggtcgacgac gcgcggcgct gcaggattcc gctggtctcg atgacaatcg
     5161 ccaagggaac ggcgcaagtc ggcagcacgc tccggatccg ttcaggcagc tatgtctcgc
     5221 cctatttcac cgtcaccgaa ggcatgcaga gaatcgcggt gccctacccc gcgccctacg
     5281 gttcgggcgc cggcaccttc gtcgtcgaag gcaatgccac cggcgctatt cttgggctta
     5341 caccgaccaa ggtgctcctg gatctgccga ccgcgcaaag cattcccgtc tggtggcgcc
     5401 cggtgagccc ctgctagccg aggcactgcc atgaagaatc ttttcaagga tcagcaaata
     5461 ctcgccatca tcgtcggcag tgtcgcggcc gcgatcatca cggcctgggt catgaatccg
     5521 caatccgggc ctgccggatg gatcaagggg ctgccgcctg cggcgcagca caccctgagc
     5581 ggcattgcca tcgcctttgt gatcgccagc gtcggattgc tggtgagctc cattgcggga
     5641 ggctacgcca ggaccggcgc gctgcaacct gcttcgagcg gcggcatgtc cggtgcgttc
     5701 aagatgctgt tttcgccgga acgggaaatt cccgacaccc cgccgaacaa agcgatcgag
     5761 gacctcgagg ccatggtcgg cctcgtgccg gtcaagcgcg aggtcaacgg attgatcgcg
     5821 cggatgcagg tcgagcaaaa gcggcgcgaa cagggcttgc cgacggcacc gttaagcctg
     5881 cacatggttt tcgcgggacc gcccggcgtc ggcaagaccg aagtcgcgcg cgcgctcggc
     5941 gacatctatc gcgcgctacg cgtgctgcgt gtcgggcacc tgatcgaagt ccagcgttcc
     6001 gacgtcgtcg ccggctacat cggacaaacc gcgccgaaga tgctggagaa gtgcaaccag
     6061 gcgctcgacg gcattctctt cattgacgag gcttacacgc tcgccgccaa cgccggcctg
     6121 ggcgccgact tcggcaagga ggcgatcgac acgctgctca aattcatgga ggaccacagg
     6181 gaccggatca tggttatcgt tgcgggctat ccgaacgaga tgcgccgctt catcgatacc
     6241 aatccaggtc ttgccggccg cttcaccaag accatcgact ttccgcgcta caatgccgat
     6301 gacctctgcg aaatccttcg gcgcatggcg gccaaacagc acttcagcct ccccgagaat
     6361 ttcccgacaa gaatcaaacc gtggatcgcg gaacgatccc gcgcggagga ttgggccaac
     6421 gcacgagaga tgcgaacgct cctggaaaaa gcccgcgagg cgcaagcgat gcgtatcgcg
     6481 acggagcctt ccgccgatct gtcgcgcatc gaaatcgtcg atttgatgaa agcaatcggg
     6541 cgcgcttgat gaggacatga tgatcacctg gctgaccatc gcattcattc tcgtcgccgg
     6601 cttcagcatg acgttcattg ccatgcgtct ggtcgaacag catgcccttc cggccgtcta
     6661 ttcgccagat ctggtgcgtc ccatccgcat cgtcgaagca gaagagagac acgagccgtc
     6721 tgcgatggag gtggcgctgg caaagctcga cgcgatggtc ggcctcgcgc cggtcaaaca
     6781 ggaagtcaag gcgctgatcg cacggatgca ggtcgaacag aagcgccggg atcagggcct
     6841 cgatgtctcg gctctcagcc agcatatggt cttcaccggc ccgcccgggg tcgggaagac
     6901 cgagatcgcg cgcctcatcg gcgagatcta ccagggcctg aaagtcctgc gcaaaggcca
     6961 tgtcgtcgaa acggatcgtg ccagcctcgt cgccggttat ctcgggcaga ccgcgatcaa
     7021 gaccctcgac aaatgccgag aggcgctcga cggcatcctg ttcatcgacg aggcctatag
     7081 tctcgccccc ggtctcggcg ccgacttcgg caaggaagcc gtcgacacga ttctgaagtt
     7141 catggaagac aaccgcgacc gcatcgtcgt gatcgttgca ggctaccgca acgagatgcg
     7201 gcgttttatc gacagcaacc cgggtcttgc cagccgcttc agcaagacga tcgacttccc
     7261 ctcctacacc accgccgaac tctgcgagat cttcaagagg atggccacgc ggcagcagtt
     7321 caacctgccg gagggtttcg aggccaggct aaaaccctgg atcgagagcc gcgcgaaggc
     7381 cgaggactgg gcaaacgcgc gcgagatgcg cacgctcttg gagagatcgc gcgaagctca
     7441 ggcgatgagg gttgctagcg acccgtccgc ggatatatcg gtggtgacca tccaggacat
     7501 tgtcctcgcc accggccagc aagacgaagg caacgaggct gcaattgcgg ctgcgctcga
     7561 caagctcaac gccatggtgg gcctcgcccc cctcaaggag gaagtgcagg cgctgatcgc
     7621 acgcaagcag gtcgagcaga agcgccgcga acagggtctc gacgtatcat ccgttagcca
     7681 gcacatggtg ttcaccgggc cgcccggcgt cggcaagacc gaagtcgcgc gggttatcgg
     7741 cgaagtcttc cgcgcgctga aagttttgcg caagggacat gtggtcgaaa ccgatcgtgc
     7801 aagcctcgtt gcgggctatg tcggtcaaac ggcgatcaaa accctcgaca aatgcaagga
     7861 ggcgctcgac ggcatcctgt tcatcgacga ggcctatagt ctcgccgcta cccccggtct
     7921 cggcaccgac ctcggcaagg aggcgatcga tacgctgctc aaattcatgg aggacaaccg
     7981 cgaccgcatc atcgtgatcg tcgcgggtta ccgcaacgag atgcggcact tcatcgacag
     8041 caacccgggc ttggccagcc gcttcaccaa gacattcgac ttcccctcct acagtcctgc
     8101 cgaactcttt gagatcttca agcggatggc cgcccggcag cagttcaacc tgccgcagga
     8161 ttttgaggcc aagctaaagc cgtggatcga gagtcgcgcg aaggcgcagg actgggccaa
     8221 cgcgcgtgag atgcgtacgc tgctggagaa gacgcgagaa gcccaggcgc tgcgcgtggc
     8281 caaggatgcg tcggcagatg tctccgtcgt caccatcgag gacgtcgttc acgcgacggg
     8341 ccagaaaatc gaggacaacg aggcgtcggt cgccgctgcg ctggcaaagc ttgacgagat
     8401 ggttggcctt gcccccgtca aacaggaagt caagcggttg tccgcaagga tgggagtgga
     8461 acagaaacgc aaggaacaag gtctccccgt tgccgcagtg agccagcaca tggttttcac
     8521 cggcccgccc ggcgtcggta aaaccgaggt tgcgcgcgtt attggagagg tcttccggtc
     8581 tctcaaggtt ttgcgaaagg gtcacctcgt cgaaaccgat cgcgcgggcc tcgttgccgg
     8641 ctatatcggg cagaccgcga ccaagaccct cgacaaatgc aaggaagcgc tcgatggcat
     8701 tctgttcatc gacgaggcct acacgctggc cgcaattcag ggtggcgctc acgacttcgg
     8761 taaggaagcg atcgatacgc tgctcaagtt catggaagac aaccgcgagc gcatcgtcgt
     8821 gatcgttgcg ggctatccca acgaaatgcg ccgtttcatc gagagcaatc cgggcttgag
     8881 cagccgcttt accaagacga tcgactttcc tccttacggc gtcgtggacc tctgcgaaat
     8941 cctcaaacgg atggcggcac gtcagaaatt ctcgctgcct gacgatttcc aggcggcgct
     9001 gacaccgtgg atcgagcagc gttcgaaggt gcaggactgg ggcaacgcgc gatcgatgcg
     9061 gacgctgttg gaaaaggcac gggagtcgca agccattcgc atttcgaccg acgcagatgc
     9121 cgacctgtca cgcatcgaga cggcggatct gttggacgcg acggtgagct gatcatgggc
     9181 gggattgtgg agagcgcctt atgaattcgg cttattcaac ctggctctcc gcccacggaa
     9241 ggctctggta cgacgacaaa ttgtatcggg cggcctggat cgcatggcct caggcgctgg
     9301 ctgccgtcat ggttttgttg ttctgggcaa tgccgccgtc caacagaaac gtgccatggg
     9361 ccaagccagt tgatgccaat gtacgctaca acgcgctgcg cacgttgcgc gacagcgcaa
     9421 aatccgaccg ccaggccatg gagacgctgg agcgagacgc ggtcgccggc gagatgaacg
     9481 cccaattctt ctacgccacc ctgttcgatc ccgatctcaa gctttcgacc atcgtacagc
     9541 cggacgtcac aaaggcggtg gattggtacg gcaaggcggc gagccaggga gatgagtact
     9601 ctataggaaa cctcgctctt acctatgcgc ggggacttta cattcgtcag gactatacaa
     9661 ggagttgttt ttacgcgcgc aaactggcca acagcattgc cttcgcaaat gccttgaacc
     9721 tcaagggaga atgctactcg cggggactgg gcggcacgcc ggtggatctg gatcaggctg
     9781 ccgccgccta tgagagtgcc gccaccaagg gccatctccg tgccaccgcc gcgctcggct
     9841 atttctatga gaacggattg ggcggtaagc cacgcaatta cgagatggcg ctgaagtact
     9901 atcgattcgc ggccgacaag ggcgactcgc tcggcctgca caatctgggt gcggcctata
     9961 attccggcct gctcggcctg cagcgcgacg gcagcgaggc agcacggctt attcttcatg
    10021 cacttgagaa caaatatgac gtcaccgtgc agtcgctaac gacacggccg gacctctgga
    10081 gtcccgattt ctggcaaaac ctgcagcgcc gcctcggcga aaaaggattt tactccggcg
    10141 tgattgacgg tcgcgccaat gctgcaacgt tggacgcggt gaaacggttg ggcggcagag
    10201 gttaatgcag aaacgagcaa tatcaggatt gtctctcttg tccgaagtgg agaagcttga
    10261 gagatttgct tgctgatcgg gaaggcaatg tgcatgcgag cggctcgatc catagcgatg
    10321 tcgacgagga tagcgatcgc tgtagcattg atcgtgatcg acccctcggg aagcttcgtg
    10381 cttgcccaga cctccctggg cggccctgaa caattgccgc cgtcgattgc gccaacgccc
    10441 ccgccgctga gcccgcctgc gaacccaccc gcgtcatcgc cgccacggat ggattacatt
    10501 cccccaatga ttccgcccaa acctgcgcca aaccaaccga gcacaacggc ggacttttac
    10561 tggggtgcga tcgccttcac cgccgacggc tcctactcgt cggtctggaa aaaatcctcg
    10621 cagccggaag ccgaagccta tgtcctgcga gagtgcgcac gcttcggacg cggtgggtgc
    10681 gaggtgacga cgttttccgg gcaggagtgc gtgggactcg cgaccttcat cggaaattac
    10741 cggcgacgac gctggaacct gtcctttacc gcgggcggct cgacttatcc cgaagcgcag
    10801 ggcgctgcgt tggagcgctg taattcggac gagcgttcgc acggtcaatg ccagcctcgc
    10861 gtggcggcat gcgccgacgg ccgatagccg ccgttcattc gattacctcg tcggcgcccg
    10921 cgagcaatgc ctgcggcaat tcgagcccga tggccttggc cgccttcgca ttgacaatca
    10981 actggaactt gcttggctga tagaagggaa tgtcgccggc cttggcgccg ttgaaaatct
    11041 ggtggacatc gcctgcgatt cgctgcgcca gctctccgag atcaggcgcg taggccatca
    11101 atccgccgag ttcgacatag tcgcggtaag gatagatgac gggcagacgg tgctcttccg
    11161 ccagctttgt cagtaacgct cgttgggcga ggaagctgcc gccttcatcg acgacggcgg
    11221 catcgaattt ctgctccgcc atctcctcga acatgcggcg gagctgcgtc tcattcacct
    11281 cgaccatctc cttcgcgacc atctccatgc ccaatcgccg tcccgcttcc tggtagccgg
    11341 cctccccggg tgaaactgtc caaacacccg aaagcaagtt tgcgaccttc ctgaccttcg
    11401 gcatcgcctc ctggacgatc tgcagccgct tgccgtaaat ttcgatgccg gcattgatgg
    11461 caacgccggt cagattgccg ccgggacgcg cgatgttgga aataaaaccc gccgcgaccg
    11521 gatcacccat gacggcgact agcggtatcg tcgaggtggc agtcatgaac gctttgacga
    11581 agtcgttcag attgacgatc acgacgtcgg gattgcggcc aacgacttct gccgcaagtg
    11641 ttgcaaagcg ctcggaacga ccttcggcag agaaacgctc gacaatgaga ttgcttccct
    11701 cgacgtcgcc cagcccggcc agcgtttcat agacctggcg tacccagaac ggacccgcgc
    11761 tcgcggtgag tttatcggcg ggaatgccgg aatgaacgaa ggcaagccga tgctgttttt
    11821 gctgggcgcg caagctgcgc ggaccgatcg aggctgccgc cgcggcgccg gcgaggccga
    11881 tgaactcccg ccgtctcatt cgatcacccg gtcggcgcgt gcgaggaaag aggacggcac
    11941 gggcgcccca cgcttcgcga ttgcggcggc gccatcaggt cgcccgacaa actcccatcg
    12001 cctcattgca ttttccccgg ccgttcaggc acttcccacc ggatttttta gccgttccag
    12061 gaagcgctgt acatatagcc gcgttgcgga catcggggtg gatcaaagac gcaagcctcg
    12121 gcataatcgg caaaaaatga aggtggggag cgctgtgacg agatcatcca aaatagcgaa
    12181 aatcgaactt ctcgcctgcg atgcaggctg gcggaattac catttcgtca aggccacgac
    12241 cgacgacggg gcggtcggct ggagcgagta cgacgaagga ttcggctccc ccggcgtcag
    12301 cgccgcgatc gagcgcctgt cgacccgggt tatcggacag aatgcgtttc agcatgagcg
    12361 tgtctacgcc gaactgttcg cggcaacgcg cccggctacc ggcggcgtgg tcgcacaagc
    12421 actcggtgct atcgagaacg cgctgcttga tgccaaggcc aagctattgg gtgtaccctg
    12481 ctatgaactg ctcggcggca aggttcgcga ttccgtccgc gtgtactggt cacattgcgc
    12541 gacctggcgc atcaatcatc ccgcctggta taagccgccg atcgagagcc tggatggcgt
    12601 gaaggcgatc ggacgcgagg tgcgcgagaa gaaattcact gcgctgaaga ccaacatctt
    12661 cacctatgag ggcggcaagc cgaccggctg gcgcccggga ttcggttcgc cg
//
LOCUS       DATLJT010000190         5097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2995759,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000190 DATLJT010000000
VERSION     DATLJT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995759"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VM910_04360"
     CDS             complement(<1..927)
                     /locus_tag="VM910_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61815.1"
                     /translation="MKRRDFILFGAGGLAALPLRAHAQGSGKVWRMGFLAHGYESFYD
                     ALFEGLRSLGYVEGKNLIVERRYAEGHPERFPDFAAEMVRLKVDIIVVSTTPAALAVK
                     NTTTAIPVVFPNAISPVESGVVASLAKPGGNITGGAAQTAILSTKRLAILKEVVPGLS
                     RGAVLWNVGNPALAYPWKESEFAAWSLDVKLQSIEVRTPEDITAALAAIAQDPPDALI
                     VLQDALTLQHRQEIIDFTAQRRLPGMFVAKEWVVAGGLMSYGENLAEMYRRGAYFVDK
                     ILKGTKPAELPVEQVTKFELVLNLRTAKAMGWT"
     gene            complement(924..1895)
                     /locus_tag="VM910_04365"
     CDS             complement(924..1895)
                     /locus_tag="VM910_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61816.1"
                     /translation="MRRRQFVTFAIGAILAWPLDLRAQQAAKTWRIGFLAHRYEKFYD
                     PLFTGLRELGYVEGQNLIVERRFAEGNAERFKEFAAEMVKLNVDAIIVVTTPAAMAAK
                     NETKTIPIIHPAAIDPVGTGLIASLAHPGGNVTGLAVLNAETSAKRVELLQEVVLGLA
                     NGAVLWNSANPANSLAWRETQDAARKLGVNLKSQEVRSPKDFDGAFAEMAEHPPEVLL
                     VVQDALTLEYRKEIIAFALQNKLPSMYVGKEWVEEGGLMSYGDRLPERYRRAADLVDK
                     VLRGTKPADLPVEQPTTFELAVNLKTARAIGLNFSPSFLARADLVIE"
     gene            2243..>5097
                     /locus_tag="VM910_04370"
     CDS             2243..>5097
                     /locus_tag="VM910_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HVI61817.1"
                     /translation="MTVGRPIVQACMRSGGGSLEACRGRAKPQVVACVIAALNAANGR
                     DNVAVAVPTEAAPKLSPGTALPAGFVAPPRTISDITAILDGEKPDPKVIEKLKSDADA
                     KPAGTESPRDLSRFYFDRSTARAQLGMVAEAIADASKGVEVGRGAIDANMMGRVKQFL
                     AVQYSNAGDPKKALEIYQQELRETNVPGAKGYQFAANRLVCNILLQMGDLAQAEGYLR
                     RSLALIQEARTSGLPGWRASYDRIGQNFEAEVEFTRAMIFEARGQFSDAEASYRATEL
                     RKSAALKPLMEGPNPPPRSNVLQTIDGAVLSQARMKARQGRLAEAEVDARRAMLSRLK
                     DTGKYNPVTPRFVMGLASILIEEGRYKEAEQLARVAIEINQTVGVPDDSQSTVQLLSQ
                     LAGILTLQRKNTEANAIYARIDQATATWEPQRRQMFELNPSRILALYDSGQVETGIAA
                     AQQLVKKLAARVGENHFDAASARGTLAIGLMRARRDAEAIREFNTAIPIMLANSSENA
                     DDENTTVVAARSQRLQTIVESYLALLARAEGATGEVGEETFGLADAIRGRSVQQALAA
                     SSARAAARDPALAELVRKEQDLTKQVNAQLGALNNVLAIPSADRDEKGARELQASIAT
                     LRTERDKARQEIKQKFPAYADLVSPKPPSVAEIRATLADGEAMLSFYFGQGGSFVWAV
                     PKSGAVAFAAVKAKAGDIETGIRKLREALEPQAAMISDIPPFDLKLAYGLYELLLKPV
                     ENGWKPARNLIVVTNGALGLLPLSLLPTAPAEVAADEDPLFVGYRKVPWLARTHAVTT
                     VPSAAALRTLRQLPPGKPGRGELVGFGDPYFNKDQETEAEGAVGKTQTADAGGNVTRG
                     VPLRRRSSPKLEGVDSAELGLLPRLPDTADELKSIALALQADPSKVLFLGKNASEGAV
                     KTMNLSGFRILAFATHGLVPGELNGLTQPALALSSPTV"
BASE COUNT          954 a         1664 c         1602 g          877 t
ORIGIN      
        1 cgtccacccc atcgccttcg ccgtcctgag attgagcaca agttcgaact tggtgacctg
       61 ttccaccggc agttcggcgg gcttggtgcc cttgaggatc ttgtccacga aatacgcgcc
      121 gcgccgatac atctcggcaa ggttctcgcc gtaagacatc aagccgccgg caacgaccca
      181 ttccttcgcg acgaacatgc ccggaagacg ccgctgcgcg gtaaagtcga tgatctcctg
      241 tcgatgctgc agcgtgagcg catcctgcag cacgatcagc gcatcgggtg ggtcctgcgc
      301 gatcgccgca agggcagcgg tgatgtcttc gggggttcgt acctcgatcg actgcaattt
      361 cacgtccagc gaccaggcag cgaactcgct ctccttccag gggtaggcaa gcgcgggatt
      421 gccgacattc caaagcaccg caccgcgcga caatccgggc acgacttcct tgaggatcgc
      481 caatcgcttc gtgctgagga tcgccgtctg cgccgcccct cccgtgatat tgccgcccgg
      541 cttggcgaga cttgcgacca cgccgctctc gaccggactg atggcattcg gaaagaccac
      601 aggtatcgcc gtcgtcgtgt tcttgaccgc aagggctgcg ggtgtagtgg agacgacgat
      661 gatatcgacc ttcaggcgca ccatctcggc ggcaaaatcc ggaaatcgct ccggatggcc
      721 ctccgcgtat cgccgttcca cgatcaggtt cttgccctcg acatagccga gcgagcgaag
      781 gccttcgaac agcgcgtcgt aaaaactctc atagccgtgg gcgaggaagc ccattcgcca
      841 gaccttgccc gatccttgcg cgtgcgcgcg gagcggcaga gccgcaaggc cgcccgcgcc
      901 gaaaaggatg aagtcgcggc gcttcattcg atcaccagat cggcgcgcgc aaggaacgac
      961 ggcgaaaagt tcaatccgat cgcccttgcg gtcttcaggt tgaccgccaa ttcgaacgtc
     1021 gttggttgct ccaccggcaa atccgcgggc ttggtgcccc gcaggacctt gtcgacgaga
     1081 tccgcggcgc gtcgatatct ttcgggaaga cggtcgccat aggacatcag tccgccttcc
     1141 tccacccact ccttgcccac atacatgctg ggcagcttgt tttgcaacgc gaaggcgatg
     1201 atttcctttc gatactcgag cgtcagggcg tcctgcacga cgagcaggac ctcgggaggg
     1261 tgctcggcca tctccgcaaa ggcgccgtcg aaatcctttg gactgcgcac ctcctgcgac
     1321 ttgaggttga ccccgagctt gcgagcggcg tcctgcgttt ccctccaggc cagcgaattg
     1381 gcgggattgg cggagttcca gaggaccgca ccgttggcga ggccgagcac cacttcctgc
     1441 aacagttcca cccgcttggc gctggtctcc gcattgagca cggcaagccc ggtcacgttg
     1501 cctccgggat gcgccaggct cgcgatcagg ccggtgccga ccggatcgat cgccgcggga
     1561 tggatgatgg gaatggtctt ggtctcgttt ttcgcggcca tggcggccgg tgtcgtcacg
     1621 acaatgatcg cgtcgacgtt cagcttgacc atctcggccg cgaattcctt gaaccgctct
     1681 gcgtttcctt cggcaaatcg ccgctcgacg atcaggttct gtccctcgac atagccgagt
     1741 tcccgcaagc cggtgaacag gggatcgtag aacttttcgt aacggtgtgc gaggaagcca
     1801 atccgccagg tctttgccgc ctgctgcgcg cgcagatcaa gaggccaggc gaggatcgcg
     1861 cctatcgcaa aagtgacgaa ctgccttcgc cgcatcgcct cccccgggga ttggacaaga
     1921 caagcccggg ccaaactagc acgagttccg gacatcggcg acgactatcc gaaagcgatc
     1981 gacgttcgcg acttgcggcg ccaactccgg agcaggattt ggctggcggc caaaaaatag
     2041 tgccttgttc tgcatcgacg cttgtcgcta gtatgcagca ttcgagccag tcattttcga
     2101 gcaaggcact tctgatatgc gatttgttgc gcgtgttatg gcaccccgcg cggttatccc
     2161 tgcactgatg gcatgcagcc tgattttatc agcgggtcat gccttcgccc tcacgaagga
     2221 agctgcgatc gagcgttgcc ggatgacggt cggaagaccg atcgtgcagg cctgcatgcg
     2281 ttccggcggc ggcagcctcg aggcgtgccg cgggagagca aagccgcaag tcgtggcctg
     2341 cgtgatagcg gcgctcaacg ccgccaatgg gcgtgacaac gttgcagtgg cggttccgac
     2401 cgaagccgcg cccaagcttt cgcccggaac cgcgctgccg gccggctttg tcgcgccgcc
     2461 gcgcaccatt tcggacatca cggcgatcct cgacggcgaa aagcccgacc cgaaggtgat
     2521 cgagaagctg aagtcggacg ccgatgccaa gccggcgggc acggaatcgc ccagggacct
     2581 gtcgcgattc tacttcgatc gctccaccgc ccgcgcgcag ctcggcatgg tggcggaagc
     2641 catcgccgac gccagcaaag gcgtcgaggt cgggcgcggc gcgattgacg ccaatatgat
     2701 ggggcgggtg aagcagttcc tcgccgtgca atattcgaat gcaggggacc cgaaaaaggc
     2761 gctcgagatc tatcagcagg agttgcgcga aaccaacgtg ccgggcgcca aaggctatca
     2821 attcgccgcc aatcggctgg tgtgcaacat cctgcttcag atgggcgatc tcgcgcaggc
     2881 ggaaggctat ctgcgccgca gcctcgccct cattcaggaa gcgcgcacca gcgggcttcc
     2941 cggatggcgg gcgtcctacg acagaatcgg gcagaacttc gaggctgagg tcgagttcac
     3001 ccgcgcgatg atcttcgagg cgcgtgggca gttcagcgac gctgaagcgt cctatcgcgc
     3061 gaccgaattg cgcaagagtg ccgccctcaa gcctctcatg gagggcccca acccgccacc
     3121 gcgatccaac gtgctgcaaa cgattgacgg cgccgtgctg agccaggcgc gcatgaaggc
     3181 gcgtcaggga cggctcgcgg aggcggaagt cgatgcgcgt cgcgcgatgc tctccaggct
     3241 caaagacacc ggcaagtaca atcccgtgac cccgcgtttc gtgatggggc tcgcgagcat
     3301 tctcatcgaa gagggccgct ataaggaagc cgagcaattg gcgcgtgtgg ccatcgagat
     3361 caaccaaacc gtcggcgttc ccgacgactc gcaatcgacc gtgcagctcc tgtcgcaact
     3421 cgccggcatt ctgaccctgc agcggaaaaa caccgaggcc aatgccatct atgcgcgaat
     3481 cgaccaggcg accgcgacct gggagccgca gcgccggcag atgttcgagc tcaatccgtc
     3541 gcgcatcctc gcgctctacg attccggcca ggtcgagacc ggcatcgccg cggcgcaaca
     3601 gctcgtcaag aaactcgccg cgcgcgtcgg agagaaccat ttcgatgcgg catccgcgcg
     3661 cggcacgctt gcgatcgggt tgatgcgcgc ccgccgcgat gccgaggcga tccgcgaatt
     3721 caacaccgcg attcccatca tgctcgccaa ctccagcgag aacgccgacg atgaaaacac
     3781 gacagtcgtt gcggcgcgga gccagcggct gcagaccatc gtcgagagct atcttgcgct
     3841 gcttgcgcgc gccgagggcg cgaccggcga agtcggagag gagacttttg gcctcgccga
     3901 cgccattcgc ggccgttcgg tccagcaggc gctggcggcg tcgagcgcgc gcgctgcggc
     3961 gcgcgatccg gcgctcgccg aactcgtgcg caaggagcag gaccttacca agcaggtcaa
     4021 cgcgcagctc ggcgctctga acaacgtgct cgcgatcccc tctgccgatc gtgacgaaaa
     4081 gggcgcccgg gagcttcaag cctcgatcgc gacgttgcgg accgaacgcg acaaggcacg
     4141 gcaggagatc aagcagaaat tccccgccta tgccgacctc gtttcgccca agccgccatc
     4201 ggtcgcggag atcagggcga ccctggcgga cggcgaggcc atgctgtcgt tctatttcgg
     4261 ccagggcggc agttttgtgt gggccgtccc caaatcaggg gcggtggcgt ttgcggcggt
     4321 caaggcgaag gccggagaca tcgagacggg gatccgcaag ttacgcgagg cgctcgagcc
     4381 ccaggcggcg atgatctcgg acataccgcc gttcgacctc aagcttgcct atgggctcta
     4441 cgagctgttg ctcaagcccg tcgaaaacgg ttggaagcca gccaggaacc tgatcgtcgt
     4501 caccaatggc gcgctcggcc tcttgccgct ttcgctgctg ccgaccgcgc ccgccgaagt
     4561 ggcggcggac gaggatcccc tgtttgtcgg ctaccgcaag gtgccgtggc tggcacgcac
     4621 ccacgccgtg acgacagtgc cttcggcggc ggcgctgcgt accctacggc aattgcctcc
     4681 cggcaaaccc ggccgcggcg aactggtcgg gttcggcgat ccctatttca acaaggacca
     4741 ggagaccgaa gccgaaggcg ccgtgggcaa gacccagacc gcggatgcag gcggcaacgt
     4801 cacgcgagga gtgccgctga ggcggcgcag ctccccgaag ctcgaaggcg tggacagcgc
     4861 cgaactaggc ctcctgcccc gccttcccga caccgcggac gagctgaaat cgatcgcgct
     4921 ggccctgcag gccgatcctt cgaaggtcct cttcctcggc aagaatgcga gcgagggtgc
     4981 ggtcaagacc atgaacctgt ccggcttcag gattctggcg tttgccaccc acggcctcgt
     5041 gcccggcgag ctcaacggtc tgacccagcc ggcgctggcg ctgtcgtcgc cgaccgt
//
LOCUS       DATLJT010000191         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_9045,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000191 DATLJT010000000
VERSION     DATLJT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9045"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..524)
                     /locus_tag="VM910_04375"
     CDS             complement(198..524)
                     /locus_tag="VM910_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HVI61818.1"
                     /translation="MPKINLSGMSVEGLMDLRERIAETLHKRRAELQKQLEALAGGRR
                     ASRGSALKGKKVPPKYRSPSGETWAGRGARPRWLVAAIKGGKKLDDFLIDKSARKGRR
                     KRRSKR"
     gene            787..1461
                     /locus_tag="VM910_04380"
     CDS             787..1461
                     /locus_tag="VM910_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI61819.1"
                     /translation="MTREVVTVGPETSVPEIAQILHAKRISGVPVVAADGSVVGIVSE
                     GDLMGHALAVGEQRGSWWLTFFSDNSALARDYTKTHGRIARDVMTQEVISIAPTASLA
                     ETARTLERNRIKRVPVVDNGKLVGIVTRANLLQALATVNVSQAASVDDRTIRERLLAE
                     LEPQPWAHLLSKNIMVQDGVVHLWGMVATEAERHALRLAAENAPGVKKVEDHLSRYPV
                     KIGGAS"
     gene            complement(1481..2467)
                     /locus_tag="VM910_04385"
     CDS             complement(1481..2467)
                     /locus_tag="VM910_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61820.1"
                     /translation="MTAKMKRRAFITLVGGAAAAWPLGARAQQAMPVIGLLSGTDREA
                     SRLDAIWRGLNEAGYVEGRNVAIEYRWAEGRLDRLPALAADLVRRRVAVIVAIQSAAA
                     SLAAKAATASIPIVFSIGGDAVKLGLVSSLNRPGGNITGTTFLYNTLAPKRLELLHEL
                     VPNAAVIGLLVNPKNPASESETSDTQAAAQTLGLKLIVQNASSSHDIDAAFASFVQQR
                     VDALTFAADAVLNNCRDQLVALAARHAVPTIYFAREFTDAGGLMSYGGDMTDAYRLTG
                     VYAGRILKGEKPADLPTQQATKVELIINLKTAKALGITVPLPLSGRADEVIE"
     gene            complement(2464..>2653)
                     /locus_tag="VM910_04390"
     CDS             complement(2464..>2653)
                     /locus_tag="VM910_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61821.1"
                     /translation="SLTELYRQIGLYTGRILKGEKPADLPVIQATKIGLTINLKTAKT
                     LGLEVPATLLALADEVIE"
BASE COUNT          509 a          778 c          822 g          544 t
ORIGIN      
        1 gcagagctag ggactaacaa ccacagcaac aagaaccgca ataataactg ctgcggcgat
       61 aaacaatcca acagccagac gctcattctt ctgacggcta ttccacacaa agaaacctat
      121 caccactagc gcaatggtga gcccaccgat aattacgcca agtgctattt gggcaatcag
      181 ttccatggag acgaaggtca ccgtttcgac ctgcgctttc tccgtccctt gcgtgctgac
      241 ttatcaatta ggaaatcatc aagcttcttt ccacctttga tggcggcgac aagccaacga
      301 ggtcttgcgc cacgacctgc ccaagtctcg cctgatggac tacgatattt cggcgggact
      361 ttctttccct tcaatgcgct cccacggctt gcccttcggc ctccggctaa ggcctccaac
      421 tgcttttgaa gttcggcccg acgtttgtgg agggtttccg caatccgctc ccgcaaatcc
      481 attagccctt caacgctcat ccctgacagg tttatctttg gcatagtgat ctccggcgca
      541 aaaacggtat agtttgtgag gctttaggga gtaaagcaat cgcaggtcgc ggtcttgaga
      601 gtgatcaaga actcgaactc ggtcggctac ttggaccggt aggcccgtag gctagacgcc
      661 ggggaaggga tctctgttct gggatatgga aggcgatcct gttcctcgct aagctgaaac
      721 tagttttgcc aagctcctgc ggaccggatg gagggaggta ccgccgtgct gactgttgcc
      781 gatgtcatga cgcgcgaggt cgtcacggtt ggacccgaga cctcggtccc agagattgcc
      841 caaatcctgc acgccaagcg catcagtggt gtcccagtcg ttgccgcgga cggaagcgtc
      901 gtcggcatcg tcagcgaggg cgatttgatg gggcacgcct tggcggtcgg cgagcagcgc
      961 ggctcgtggt ggttgacgtt cttttcggat aacagcgcgc tcgcgcgtga ctatacgaaa
     1021 acccacggcc gcatcgcgcg cgatgtgatg acgcaggaag tgataagtat cgcccccacg
     1081 gcatcgctcg ccgagactgc caggacccta gagcgcaacc gcatcaagcg cgtgccggtg
     1141 gttgacaatg gcaagctggt ggggatcgtg acgcgcgcca acctgttgca ggcattggcg
     1201 accgttaatg tatcacaggc ggcgagcgtc gatgatcgca ccattcgcga gcggctgtta
     1261 gcagaacttg aacctcaacc ctgggcgcac ctgctgtcga agaacatcat ggtgcaggac
     1321 ggtgtcgtgc acctgtgggg tatggtcgca accgaggcgg agcgtcacgc gctgcgcctg
     1381 gcggcggaga acgcgcccgg cgtaaagaaa gttgaggacc acctgagcag gtacccggtg
     1441 aagatcgggg gcgcgagcta ggcatcaggt cggcgtctct tcattcaatc acctcgtcgg
     1501 cgcggccgga caggggcaga gggacggtga taccgagcgc cttggcggtc ttgaggttga
     1561 tgattagctc gactttcgtg gcctgctgcg tcgggaggtc cgcgggtttc tctcctttga
     1621 ggattcgacc cgcgtagacg ccggtcagac gataggcatc ggtcatgtcg cccccatagc
     1681 tcatcagacc tccggcatcc gtgaactcgc gcgcaaagta gatggtgggc accgcgtggc
     1741 gagccgccag cgcgacgagc tggtcgcggc agttgttcaa gaccgcgtcg gcggcgaagg
     1801 tgagcgcgtc gactcgctgt tgcacgaaac tcgcgaaagc cgcgtcaatg tcgtggctgc
     1861 tgctcgcgtt ctggacgatg agcttcagcc ccagcgtctg tgccgccgcc tgcgtgtcgc
     1921 tggtctcgga ctcggaagct ggatttttcg gattgaccag gagtccgatc acagccgcgt
     1981 ttggtaccag ctcgtggagc aactctagcc gtttgggcgc gagcgtgttg tacaggaagg
     2041 tcgttccagt gatattgccg cccggtcggt taaggctgga aacgagaccg agtttgaccg
     2101 cgtctccacc gatcgagaac acgatcggaa tgctcgccgt tgcggctttg gcggcaagtg
     2161 acgccgccgc gctctggatc gcaacaatca cggccacgcg gcggcgaacc aggtcggcgg
     2221 ccagtgctgg caatcggtcg agacgaccct cggcccagcg atattcgatc gcgacgttgc
     2281 ggccctcgac atagccggct tcgttgagac ctcgccagat ggcgtcaagt cggctggctt
     2341 cacggtccgt gccgctgagc aacccgatca ccggcatggc ctgctgcgcg cgcgctccga
     2401 gcggccacgc cgccgccgcg ccgccgacga gcgtgatgaa cgcgcgccgc ttcatctttg
     2461 cggtcattcg atgacctcgt cggcgagtgc gagcagcgtt gctggcactt ctaggccgag
     2521 tgtcttggcg gtcttgagat tgatagttaa cccgattttc gtggcctgta tgacgggcag
     2581 atcggccggc ttttcaccct tgagaatgcg acccgtgtaa agtccgatct gtcggtaaag
     2641 ctcggtcaga ctc
//
LOCUS       DATLJT010000192         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4374474,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000192 DATLJT010000000
VERSION     DATLJT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4374474"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VM910_04395"
     CDS             complement(<1..176)
                     /locus_tag="VM910_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HVI61822.1"
                     /translation="MRQSSLAVLPAFKDFGNHAMTMTRRGFLSASAALASAPAWSGGA
                     FSAALPRDADIVVIG"
     gene            319..1308
                     /locus_tag="VM910_04400"
     CDS             319..1308
                     /locus_tag="VM910_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HVI61823.1"
                     /translation="MGFVLDTIGKLIAGYLQKEVPGYEPFTPSDPEYLRSVIEPGDVL
                     LVEGNNRISGIIKYLTQSTWSHAALYVGPIDGAFEEDGESHVLIEANVGEGVSSAPLS
                     KYFTYHTRLCRPVGLSYEDRNTVCRYAINRIGFGYDTKNILDLMRYLFPLPVPQRWRR
                     RMIALGSGDPTKMICSALIAQAFDAVRYPILPKITRAGSRQARREILHIRDSSLYMPR
                     DFDISPYFEIVKPTIVRGFDYTTLHWADKQKPLQEVAGEFGPFPETVGSPPLVPEEID
                     AEAPLAAEEVMLDAEATMLIERFIVEEHFLRVEYVPARLPERRRKQRERLLVA"
     gene            complement(1305..1580)
                     /locus_tag="VM910_04405"
     CDS             complement(1305..1580)
                     /locus_tag="VM910_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HVI61824.1"
                     /translation="MRRDIKSSVQKRLSRIEGQVRGLSRMVDEDRYCIDVVTQISAVR
                     AALRRVEEEVLQDHVAHCVEHAISSGNKADQRQKITELMAVIGRSDR"
     gene            1633..>2627
                     /locus_tag="VM910_04410"
     CDS             1633..>2627
                     /locus_tag="VM910_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HVI61825.1"
                     /translation="MSEAVSEHSGAGNNCCAGHGDHAAPSHDHLHRADAAQTVRDPVC
                     GMSVDPATSKHRFEHKGETFHFCSAGCRTKFETDPAKYLEKKTTAPANVPAGTIYTCP
                     MHPQIRQVGPGTCPICGMALEPEVASLEAEPNPELTDMTLRFWIGALLSVPPVVLEMG
                     GHLAGPHKWIDPTLSNWIQLVFATPVVLWAGWPFFVRGWQSLVTRNLNMFTLIAMGTG
                     VAYAYSVIGTVAPQIFPATFRDHSGAVAVYFEAAAVITVLVLLGQVLELRARAATSGA
                     IKALLQLAPKTARRVGDDGIDHEVEIDQLKVGNRLRVRPGEKVPVDGIILEGRSSL"
BASE COUNT          501 a          834 c          788 g          504 t
ORIGIN      
        1 ccgatcacca caatgtcggc atcgcgcggc agggccgcgc tgaaggcgcc ccccgaccag
       61 gccggagcgg atgccagtgc cgcggacgcc gaaagaaagc cgcggcgcgt cattgtcatg
      121 gcatggtttc cgaaatcttt gaaagcgggc agaacagcca gcgaactttg ccgcatctga
      181 tgcggcacgg caaccatgat ggtgaatcaa tcatgattga tgtcgtttcc gcatgaacca
      241 aattgcaacg gctttcgagc atgataagag aagggaaaaa tcggccggca aggccgtccg
      301 gccgggggga ttttcatcat gggcttcgtg ctcgacacga tcggcaagtt gattgccggc
      361 tacctgcaaa aggaggtgcc gggatacgag ccgttcacgc cctccgatcc ggaatatctg
      421 cgcagcgtca tcgagccagg cgatgtgctg ctcgtcgagg gcaacaaccg catctccggc
      481 atcatcaaat atttgacgca gtctacctgg tcgcacgccg cgctctatgt cggaccgatc
      541 gacggtgcat ttgaagaaga cggcgagtcc catgtgctga tcgaggccaa tgtcggcgaa
      601 ggcgtcagtt cggctccact ctcgaaatat ttcacctatc acacccggct ctgccgacct
      661 gtcggcctgt cctatgagga tcgcaacacg gtatgccgtt acgcgatcaa ccgcatcggc
      721 ttcggctacg acaccaagaa cattctcgat ctgatgcgct atctgttccc cctgccggtg
      781 ccgcagcgct ggcggcggcg gatgatcgcg ctcggctcgg gtgatccgac caagatgatc
      841 tgctcggcgc tgatcgcaca ggccttcgat gcggtgcgct acccgatcct gcccaagatc
      901 acgcgagccg gctccaggca ggcccggcgc gagatcctgc acatccgcga ttcctcgctt
      961 tacatgcccc gcgatttcga catctcgccc tatttcgaaa tcgtcaaacc caccatcgtg
     1021 cgcgggttcg actacaccac gctgcactgg gccgacaagc aaaagccgct ccaggaggta
     1081 gcgggcgagt tcggaccgtt tcccgaaacg gtcgggtcgc cgccgcttgt tcctgaagag
     1141 attgacgcgg aagcgccgct tgcggctgag gaagtgatgc tggatgctga agccacgatg
     1201 ctgatcgagc gcttcattgt ggaggagcac ttcctgcgcg tcgaatatgt tccggcgcgg
     1261 ctgcccgagc gccgcaggaa gcagcgggaa agacttctcg tcgcctagcg gtcggagcga
     1321 ccgatcacgg ccattagctc ggtaatcttc tgtcgctgat cggccttgtt gccgcttgaa
     1381 atcgcgtgct ccacgcagtg cgcgacgtga tcctgcaaga cctcctcctc gacccgccgc
     1441 agcgccgcgc gcaccgcgga aatctgcgtg acgacgtcga tgcaataacg gtcctcgtcg
     1501 accatcctgg agagtccgcg gacctgccct tcgatccgac tcagacgctt ttgaacggat
     1561 gacttgatgt ccctgcgcat gaggcctata tacccccaca gggtataggt ttcaagaggc
     1621 cggagcgtgc cgatgagcga agccgtttct gagcattcag gcgcgggcaa caactgctgc
     1681 gccggacacg gcgatcatgc cgccccctcg cacgaccatc tccatcgcgc ggacgccgcg
     1741 cagacggtgc gcgatcccgt ctgcggcatg agcgtcgatc cggcgacgtc aaagcaccgc
     1801 ttcgagcaca aaggcgagac ctttcatttc tgttcggctg gctgccgcac caaattcgag
     1861 accgatccgg caaaatatct ggaaaagaag acgacggcac cggccaacgt gcctgccggt
     1921 acgatctaca cttgcccgat gcatccacaa attcgccagg tcggacccgg cacctgcccg
     1981 atctgcggca tggcgctgga gcccgaggtc gcaagcctgg aggctgagcc caatcccgaa
     2041 ctcaccgaca tgacgctccg cttctggatc ggcgctctgt tatcggtgcc gccagtggtt
     2101 ctggagatgg ggggccatct cgccggcccg cacaaatgga tcgatcccac gctgtcgaac
     2161 tggatccagc tcgttttcgc gacgccggtg gtgctgtggg ccggctggcc gttcttcgtt
     2221 cgcggctggc aatctcttgt gacgcgcaat ctcaacatgt tcactttgat cgccatgggt
     2281 acgggcgtgg cttacgccta cagcgtgatc ggaacggtcg cgccgcagat ctttcctgcg
     2341 actttccgcg atcattccgg cgcagtcgcg gtgtatttcg aggcggccgc cgtgatcacc
     2401 gtgctcgtcc tgctgggtca ggtgctggag ttgcgcgcac gcgccgcgac ctcgggcgcg
     2461 atcaaggcgc ttttgcaact cgcgccaaag actgcccgcc gcgtcggcga tgacggcatt
     2521 gaccacgagg ttgaaatcga tcagctcaag gtcggcaaca ggctgcgcgt gcggccgggc
     2581 gaaaaagtgc cggttgacgg catcattctc gaaggccgct cctcgct
//
LOCUS       DATLJT010000193        10094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3455395,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000193 DATLJT010000000
VERSION     DATLJT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455395"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VM910_04415"
     CDS             complement(<1..923)
                     /locus_tag="VM910_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61826.1"
                     /translation="MPFPCAARRSLDLRGRQQVAFVVALPHAAEPSPSRASLNGLDAI
                     NFFNAAMLAGFGPYVAVYLAEQKWTQENIGFVLTASGLAGLLALMPGGELLDTVRSKR
                     ALAALGASMVAVTALAIGIWPDFPVVFTALVLHGTTGAFLGPAIAAISLGVVGHLALP
                     EQLGRNQRFASAGSLIATGLMGLIGYLMTYQAMFLIAAALGLPLLVALAAMRPADIDF
                     ARSCGAAHPDVPTQRARTTHRCLWKNPAVLTFAAGLFLFQLANAAMLPVVGAALVYDG
                     ESRSSLIVAALIILPQIVVALLAPWVGRQARR"
     gene            complement(1756..4578)
                     /gene="treY"
                     /locus_tag="VM910_04420"
     CDS             complement(1756..4578)
                     /gene="treY"
                     /locus_tag="VM910_04420"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HVI61827.1"
                     /translation="MPPSVPLATYRLQLTPNLGFDQAAALVPYLKALGISHLYASPFL
                     KARSGSPHGYDVVNHNSFNPELGGEDAFRRLSAALDTADIGLILDFVPNHMGVHYADN
                     PWWLDALEWGPKSPCAASFDIDWQTLPAHPGGGVLIPVLGTSYGEALERGDIELRYDA
                     AEGSFSAWYYEHRLPIAPSRYGEILQKVVSEAGAGERPAGRKLLEFAARYRGPHNPPR
                     QQAPAFKAELASITDGKDVIERGLRAYRPTREPGAILALHHLLERQHYRLAHWRLAGS
                     EINYRRFFDINTLAGLCVEDRDTFDAIHGLVRRLVTEGCLHGLRLDHIDGLRDPHQYF
                     RRLQRLIDVARPSKNRPFYVMAEKILADGERLPRLAGVAGTTGYEWLNVISRLLLDDR
                     GLATLGRTWHDVSGEERGFDAILIDAKHRVITNILASEFTVLTRLLARIAAGHYTTRD
                     YTAERLRAAFELFILHFPIYRTYVTASGPSREDRTIIEGALANARADWFGSDLGIFEF
                     LRDALTLDLIAPGRAGHSIARDRRFAFKVQQFTGPMMAKSLEDTALYRFHRLLALNEV
                     GGDPAAGGLSVASFHERIQERATKWPHGLTATATHDTKRGEDARARLIALSELAEEWT
                     RSVHQWRQLNAKLVVSSGPSRIPSAAHEYMLYQALLGAWPLAGIDAGFLERVQAYAVK
                     AAREGKQQTSWLAPNEPYEAGLKDFLDRLLDRNHSASFIEAFDAFARRAALMGALNSL
                     AQLVLKAAMPGVPDFYQGSEFWDLALVDPDNRRPVDFAARASALNAIGQPPDWRALAS
                     AWPDGRVKFALTRQLLTLRQQLPDVFTNGSYRPLSVVGPNRHEIVAFARLGEQHAVIV
                     VSGRLFGRVTQGGRRWPPGQAWNASVLAEGFSAMRTVLTVGKTMTGPSLAAADLFDVL
                     PIAVLQARYAPAKRERTRTKVSAAAK"
     gene            complement(4578..6341)
                     /gene="treZ"
                     /locus_tag="VM910_04425"
     CDS             complement(4578..6341)
                     /gene="treZ"
                     /locus_tag="VM910_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HVI61828.1"
                     /translation="MASARFGAELHQSGVTFRLWAPAAKRVDVMLDRPYPMQAQTEGW
                     YEVTIPGARAGTLYKYRIDGEMEVPDPASHFQPHDVLGPSEVIDHDRFEWRTHGWPGR
                     PWEDAAFLEVHVGTFTSGGTFRTAIERLDHLVETGVTAIELMPIADFAGRRNWGYDGV
                     LLYAPDSAYGRPDDLRTLIDGAHAHGLMVFLDVVYNHFGPEGNYLHHYAPTFFTSAHT
                     PWGHAINYNIPQVRAFAIGNALHWLERYRFDGLRLDAVHAIVEAGQPSILHDLSRVVG
                     HFAAASRRTIHLVLENDDNRASLLDPCANPAQGKYRAQWNDDYHHAWHRLLTGEARSY
                     YQDYAPDPRRHIARVLSSGFAYQGEPSPHRRGRSRGEASRALSPLAFVNFLQNHDQIG
                     NRPLGDRLAAHVHEAALTAALAITLLAPMPPLLFMGEEWGSTQQFPFFCDFHGALADA
                     VRKGRREEFKSAYAELSDKVPDPLDETTFRSAALDWDARMTLDGRKRLALVRDLLTTR
                     QREIVPQLAGAKFGAAQCEQGILTANWVLAHGKTLRMLANLADDGTELPRNFRPLRPI
                     WGGDPPAKLPPWSVFWSIGEG"
     gene            complement(6341..8413)
                     /gene="glgX"
                     /locus_tag="VM910_04430"
     CDS             complement(6341..8413)
                     /gene="glgX"
                     /locus_tag="VM910_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HVI61829.1"
                     /translation="MRISAGAPHPLGARWDGRGTNFALFSANAEMVELCLFDSHGRRE
                     IERIALPERTEDVWHGYLNDVAPGQLYGYRVHGLYQPERGLRFNAHKLLVDPYARQLA
                     GRLVWSDAHFGYRAGSARADLSFDRRDNARGIPKSVVVDEAFTWGEEHRPSIPWQGTI
                     IYEAHVKGLTQLREDVPPAWRGTYRGLTAPAVVDHLKRLGVTAIELLPIHAFIDDRHL
                     VERGLSNYWGYNTLGFFAPEMRYAADGPLDAFRSTVSRLHDAGIEIILDVVYNHTAEG
                     NHLGPTLSFRGIDNTSYYWLLPDQPRYYDDFTGCGNALKLTHPRVLQMVMDSLRYWVQ
                     VCHVDGFRFDLASTLGRGSNGFDGGTAFFAAIRQDPVLAGVKLIAEPWDLGPGGYRVG
                     GFPSGWSEWNDRFRGTLRRYWSGEGNLIGEVGRRMTGSADLFHHDGRGPRSSINYVTV
                     HDGFTLADLVSYERKHNEANGEHNRDGFDENHSTNYGVEGPTDDPAILQLRRQLQRNL
                     LASVMLAQGVPLLLAGDEVGNSQGGNNNAYCQDNPIGWVDWSALGRNEEDMTALVSEL
                     SELRRRFPQLRAQRWVEGRRTDGSFGVLWLTPQATEMVEQDWNFPQGRFLSYVLGPVE
                     QADAPLYIVLNAALETIEFTLPTLPQYRRWTALLETAPEPRLGAQFASGSKLQARPRS
                     ILVFSGSP"
     gene            complement(8415..>10094)
                     /gene="glgB"
                     /locus_tag="VM910_04435"
     CDS             complement(8415..>10094)
                     /gene="glgB"
                     /locus_tag="VM910_04435"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HVI61830.1"
                     /translation="MRVRGNGFWEIFVPEARAGDKYKYEIVGPDGHLLPLKSDPLAFA
                     AELRPRTASIVVDRETIQRPQPAPDGINALGAPISIYEVHLGSWRRHAQHGGRWLSYR
                     ELAEELPAYAGDMGFTHVEFLPASEHPFDGSWGYQPTGLFAPTSRFGTPADFAVLIDG
                     CHRKGLAVIVDWVAGHFPDDPHGLSRFDGTALYEHENPKQGRHLDWNTLVYNYGRTEV
                     ANFLLGSGLFWLDRYRIDGLRVDAVASMLYLDYSRPEGGWIPNKYGGRENLDAINLLR
                     RFNSELFSRFPNATTAAEESTAWPMVSRPVDRGGLGFGYKWNMGWMHDTLEYISKDPI
                     YRKHHHGQILFGLHYAFFENFILPLSHDEVVHGKRSILGRMPGDEWQRFANLRAYYGF
                     MFGHPGKKLLFMGSEFGQESEWRHDHSLDWHLLKHARHAGVQALVRDLNRLYRTIPAL
                     HELDCEDAGFEWLVMHDAERSVFAWLRKGRQTHERCLVVVNFTPEIYRDYRIKVPFSG
                     TWREVLNSDSALYGGSNVGNAGAVTTLDEGAIPEVSLVVPPLATIFLVPER"
BASE COUNT         2054 a         3035 c         3146 g         1859 t
ORIGIN      
        1 cgtcgcgctt gccgtccaac ccaaggagcc aacagagcaa cgacaatctg cggtaggatg
       61 attaatgcag caacgataag cgaggagcgg ctttctccgt cataaacgag agcagctccc
      121 actactggta gcatggcagc attggcgagt tgaaacagaa acaagccagc ggcaaaggta
      181 agcaccgccg ggttcttcca aagacatcga tgcgtggtcc tcgcgcgctg agtcggcaca
      241 tcgggatggg ccgcgccgca ggagcgcgcg aagtcaatgt cggctggacg catcgcagcg
      301 agtgcaacga gcagtgggag gccaagcgcg gcagcgatca ggaacatcgc ttggtacgtc
      361 atcaaatatc caatcaggcc catgagccct gtcgcgatca gcgatccggc ggaagcaaag
      421 cgctgattgc gccccagctg ctcgggcaaa gccaaatgac cgactacgcc aaggctgatg
      481 gcagcaatcg ctggcccaag gaatgcgcct gtggttccgt ggagcaccag ggcagtgaaa
      541 accaccggaa agtccggcca aattccaata gcaagtgcgg tgacagcaac catgctggcg
      601 ccgagcgctg ccagagcccg cttcgagcgg actgtatcca gcagttcgcc acccggcatg
      661 agggcaagca agccggcgag accgctcgcg gtgaggacga agccgatatt ctcctgagtc
      721 catttctgct cagccagata aacggccaca tagggaccga aaccagccaa catggccgca
      781 ttgaaaaaat tgattgcgtc aagaccgttc aggctcgcac gagatggcga gggttcagca
      841 gcatggggca acgccacaac gaatgccact tgctgacgtc ctctcaaatc aagggatcga
      901 cgggcagcgc acgggaacgg cacggccttt tccgctgtgg cgccttcgca gcggcccatg
      961 tgcgacgccc gtcaatccaa aatattcggg agcggtattc cgatcgtgtc cccccgggcc
     1021 cgaatacctg gacgcttaac gtccagccgc ttggtcggtt ccgcccgcac cgtagttctc
     1081 tgttagcccg atccgcattg cccaacagtg tggcccgctt gcaacatacc gcgggttaag
     1141 ttgcccgtct gcttcgacga tttgtagatg caacaacgtg taggtcgggc ggttggcgag
     1201 cagcacttag ccacattgcg gagcggaggc actccgattg cgtcaactgg ctactgccgg
     1261 ccagcggcac cacatcaaag gtagtgaaaa accggtcggt ctcgggtcgt gccgccctct
     1321 cggaaatcct ccaagtacaa gtaacggaga gcggtcgcca gcgagcttag gacggagagc
     1381 aggggggctc tcgcttgatt ctaaagaaaa gccccgtctt agaggaacgg ggcccgagtt
     1441 tgactagggt gagacacttg aagtctcctg cctcacccat caccccaacc cccaacctcg
     1501 cattcggttc cggcgccaga cctgccggcg cggatccgaa caactgttac aaccttgatc
     1561 cgcggggcaa tggttgaaga tgagaaacga tttgtcagct tccggttgcc cgaatctgtc
     1621 atctcgcgta catagcgatt cggcggaggg agggaaccga cccttggtcg gtcggcacga
     1681 cgtccgcgag gccgttcggc cctcacgcga gatcccggac gccccctttc tttcctatga
     1741 cgcttgcgcg cccgcttatt tggcggcggc gctcacctta gttcgcgtcc gttcgcgctt
     1801 ggctggcgca tatcgcgctt gcagaacagc aattggcaga acatcgaaca agtctgcagc
     1861 cgccaagctt ggcccagtca tcgttttgcc gaccgtcagt actgtgcgca tcgcggaaaa
     1921 accttcggcc aataccgatg cattccaggc ttgaccgggc ggccatcgcc ggccgccctg
     1981 cgtcacgcga ccaaatagcc gtccgctcac gacgataacg gcgtgttgtt cgccgagccg
     2041 tgcgaacgcg acgatctcgt ggcggttcgg accgacgaca gagagcggac gataggagcc
     2101 attggtaaag acgtccggaa gttgctgtcg cagcgtaaga agctggcgcg tcagggcgaa
     2161 tttgacgcgg ccgtctggcc atgcgctcgc aagcgcacgc caatccggcg gctggccaat
     2221 cgcattgagc gcggatgctc gcgcggcgaa gtcgaccggt cgtcggttat cgggatccac
     2281 caacgccaag tcccagaatt cggagccctg atagaaatcg gggacgcccg gcatggccgc
     2341 cttcaggact aactgcgcga ggctgttcag cgctcccatc aatgctgcac ggcgcgcgaa
     2401 tgcatcaaag gcttcgatga agcttgcgga gtggttgcga tcgagaagcc gatcgagaaa
     2461 gtctttgagt cccgcctcgt aaggttcatt gggcgcgagc caactcgtct gttgtttgcc
     2521 ttcgcgggcg gctttgacag cataggcctg aacccgctcg aggaagcccg cgtcgatgcc
     2581 ggcaagaggc caggcgccga gcagtgcctg atagagcatg tattcgtgcg ctgccgaggg
     2641 tattcgtgac ggtcctgaag agacgacgag cttagcgttg agttgccgcc attggtgcac
     2701 actgcgcgtc cactcttcgg cgagctcgga aagggcgatt agtctcgcgc gcgcgtcctc
     2761 cccgcgcttg gtgtcgtgcg ttgcagttgc ggtcaggcca tgcggccatt tggtagcgcg
     2821 ctcctggatt cgctcgtgaa agctcgcgac ggacagccca ccggcggcgg gatcgccgcc
     2881 gacttcgttg agggccaaaa ggcgatgaaa gcgatagagc gccgtatcct cgagggactt
     2941 tgccatcatc ggaccggtga attgttgaac cttgaaggca aagcggcggt cgcgtgcaat
     3001 gctatggccg gcgcgccccg gggcaataag gtcgagcgtc aatgcatcgc gcaggaactc
     3061 gaaaatcccc aagtccgaac cgaaccaatc ggcgcgagca ttggcgagcg ccccttcgat
     3121 gatcgtacga tcctcgcggc ttggccctga cgcggtaacg taggtgcgat agatcggaaa
     3181 atgcaaaatg aacagctcga acgcggcgcg caggcgctcg gcggtatagt cgcgtgtggt
     3241 gtaatgaccg gcggcgatcc gcgcgagcaa acgcgtcaat acggtgaact cgctcgccaa
     3301 aatatttgta atcacacgat gcttggcgtc gatgagaatg gcgtcaaaac cgcgctcttc
     3361 gccgctcacg tcgtgccagg tccgcccaag cgtggcgagt ccgcgatcgt cgagaagaag
     3421 ccgggagatg acgttgagcc attcatagcc tgtcgtgccg gcaactccag caaggcgagg
     3481 taggcgctct ccatcggcca gaatcttttc ggccatcacg tagaacggtc tgttctttga
     3541 gggccgtgcg acgtcgatga gccgttgcag ccggcggaaa tactggtgcg gatcgcgcag
     3601 cccatcgata tgatcgagcc gtaacccgtg caggcagccc tcggtaacaa ggcggcgaac
     3661 aagcccatga atggcgtcga aagtatcgcg gtcttccacg cacagcccgg ccagtgtgtt
     3721 gatgtcgaaa aaccgccggt agttgatctc gctgccggca agtcgccaat gcgcgagccg
     3781 ataatgctgg cgctcgagca agtgatgcag ggcgagaata gccccgggct cgcgagtcgg
     3841 gcgataagcc cgtagcccgc gctcgatcac gtccttcccg tcggtgatcg acgcgagttc
     3901 ggccttgaag gccggcgctt gctggcgcgg gggattgtgc gggccgcggt accgcgccgc
     3961 gaattcgagc agcttgcggc ctgcaggccg ctcgcccgcg ccggcttccg atacgacctt
     4021 ctgcaaaatc tcgccgtaac ggctgggagc gatgggcagc cgatgctcgt aataccaggc
     4081 ggagaagctg ccctctgctg catcatatcg aagctcgatg tcgccgcgtt ccagcgcctc
     4141 gccgtaggac gttccaagaa ccgggatcag gacgccgccc ccaggatgcg cgggcagggt
     4201 ctgccaatcg atatcgaacg acgcggcgca gggagatttc ggtccccatt ccagggcgtc
     4261 gagccaccaa ggattgtccg catagtgaac gcccatgtgg ttgggcacga aatcgagaat
     4321 gagcccaatg tccgccgtat cgagcgcagc ggacaggcgc cgaaacgcat cctcgccgcc
     4381 gagctccggg ttgaacgaat tgtggttgac cacgtcatat ccgtgcgggc tccccgaacg
     4441 cgccttgagg aacggggaag catagagatg gctgatgcca agggccttga gatacggcac
     4501 cagcgcggcc gcttggtcga agcctaaatt cggagtgagt tggagacggt aagtcgcgag
     4561 cgggaccgag ggcggcatca tccctctccg atgctccaga acaccgacca cggcggcagc
     4621 tttgccggcg gatcgccgcc ccaaatcggt cgtagcggtc gaaagttgcg cggtagctcg
     4681 gtgccgtcat cggcgaggtt ggcaagcatc ctcagtgtct tgccgtgggc gagtacccaa
     4741 ttcgcggtca ggataccttg ctcgcattgt gcggcgccga actttgcacc ggcaagctgc
     4801 gggacgatct cccgctgtcg tgtcgtcagc aggtcgcgga ccagcgcgag ccgcttgcgg
     4861 ccgtccaacg tcatgcgcgc atcccagtcc agggccgcgg agcgaaacgt tgtttcgtcg
     4921 agcgggtccg gaaccttatc gctcaattcg gcataggcgg acttgaattc ctctcgtcgt
     4981 cctttgcgca ccgcatccgc cagagccccg tggaaatcgc aaaagaaggg aaactgctgg
     5041 gttgatcccc attcttctcc catgaacaag agcggtggca tcggcgcaag cagcgtgatg
     5101 gcgagcgccg ctgtcagagc agcctcgtgg acgtgcgccg caagacgatc ccccaggggg
     5161 cgattgccaa tctggtcatg gttctgcagg aaattgacga aagcgagcgg cgatagggca
     5221 cgcgatgctt cgccgcggct tcgaccgcgc cggtgaggcg agggctcgcc ctgataggcg
     5281 aagcccgagg ataggacgcg cgcgatgtgg cggcgaggat ccggtgcgta atcctggtag
     5341 tagctcctgg cctctccggt caacaggcgg tgccaagcgt gatggtaatc gtcgttccat
     5401 tgcgcgcggt atttaccttg cgcaggattg gcgcacggat cgagtaaact tgcgcggttg
     5461 tcgtcgttct ccaggacgag atgaatcgtt cgcctggagg cagcagcgaa gtgcccgacc
     5521 actcggctca ggtcatggag gatcgagggc tgtccggctt cgacgatggc atgaacggca
     5581 tcgagccgaa gaccgtcgaa acgatagcgc tcaagccagt ggagcgcatt accgatcgcg
     5641 aaggcccgca cctgtggaat gttataattg atggcgtggc cccatggcgt atgagcagag
     5701 gtgaagaatg tcggcgcgta atggtggaga tagttacctt ccggaccgaa gtgattgtag
     5761 acgacgtcga gaaacaccat gagcccgtgc gcatgcgctc cgtcgatcag cgttcgcaga
     5821 tcatccggcc gcccataggc gctgtccggc gcataaagca ggacgccgtc gtagccccaa
     5881 tttcgccggc cagcgaagtc ggcaatgggc atcaattcga tcgcggtcac tccggtctcg
     5941 accaggtggt cgagcctttc gatggcggtg cgaaacgtac cgccggaggt gaaggtgccg
     6001 acatgaacct ccaaaaacgc cgcatcctcc cagggccgcc ccggccatcc atgtgtccgc
     6061 cactcgaatc gatcgtggtc gatgacttcg cttggcccga gcacgtcatg cggttggaaa
     6121 tgcgaggccg gatctggaac ctccatttcg ccatcgatgc gatacttgta gagcgtcccg
     6181 gcgcgcgcgc ctggtatcgt gacttcgtac cagccttccg tctgcgcctg catgggatac
     6241 gggcgatcca gcatcacgtc gacgcgcttg gcggccggag cccagaggcg gaaggtgacg
     6301 ccactttgat gcagttcggc gccaaagcgc gcgctggcca ttatggcgac cccgaaaata
     6361 ccagaattga tcgtggccgc gcctgcagct tcgagccgga cgcgaactgt gcgccgagcc
     6421 gcggctcggg cgcggtttcc aagagcgccg tccaacggcg gtattgcgga agggtaggaa
     6481 gcgtgaactc gatcgtctct agcgcggcgt tgagcacgat atagagcggc gcgtccgctt
     6541 gctccactgg accgaggaca taggacagaa agcgtccttg cggaaaattc cagtcctgtt
     6601 ccaccatctc ggtggcttgt ggcgtcagcc acaacacgcc gaatgagccg tcagtgcggc
     6661 ggccttcgac ccagcgctgt gcgcgcaatt ggggaaatcg ccggcgcagc tcgctgagct
     6721 cgctaaccag tgccgtcatg tcctcctcat tgcggccgag cgcggaccaa tccacccatc
     6781 cgattggatt gtcctggcaa taagcgttgt tgttgccacc ctggctattg ccgacttcgt
     6841 ctcccgcgag cagcaacggc acgccttgcg ccagcatcac gcttgcaagc agattacgtt
     6901 gcaattgccg cctaagctgg agaatggcgg gatcatcagt cggtccttca acgccataat
     6961 ttgtgctgtg attttcatcg aacccgtctc ggttgtgctc gccgtttgct tcgttgtgtt
     7021 tgcgctcgta gctgacgaga tccgcaaggg tgaacccatc atgaaccgta acgtagttga
     7081 tgctcgagcg cggacctcga ccgtcgtggt gaaatagatc ggctgagccg gtcatgcggc
     7141 ggccaacctc gccgatgaga ttgccttcgc cggaccagta gcgacgcagc gtgccgcgaa
     7201 agcggtcatt ccactccgac cagccggatg gaaacccgcc gacccgatag ccgccgggac
     7261 caagatccca cggttcggcg atcagcttga ctccggccaa aacagggtcc tgccggatgg
     7321 ctgcaaagaa ggccgtgcca ccatcaaaac cgttggagcc gcggccgagt gtgcttgcga
     7381 gatcgaacct gaagccgtcg acgtgacaaa cctgcaccca ataccggagc gaatccatga
     7441 ccatctgcag cacgcgtgga tgcgtgagct tgagtgcatt cccgcagccc gtgaagtcgt
     7501 cgtagtagcg gggctgatcg gggagcagcc agtaatacga ggtattgtcg atgccgcgga
     7561 agctcagagt cgggcctagg tgattgcctt cggcggtgtg gttgtagacg acgtcgagga
     7621 tgatttcgat gccggcatcg tgcaatcgcg agacggtaga tcgaaacgca tcgagcggtc
     7681 cgtccgcggc ataacgcatc tcgggcgcga aaaagcccag cgtattgtag ccccaatagt
     7741 tgctgaggcc gcgctcaacc agatgccgat catcgataaa agcgtgaatc ggcagcagtt
     7801 caattgcggt gacgcccaga cgcttgagat gatcgaccac cgccggtgcc gtcagcccgc
     7861 ggtatgtccc gcgccaagcg ggcggcacat cctcgcgaag ttgagtcagt cccttgacgt
     7921 gggcctcgta gatgatggtg ccttgccagg ggatcgaggg tcgatgttct tcgccccatg
     7981 tgaacgcttc gtcgacgacc acgcttttcg gtattccacg cgcgttgtcg cgccggtcga
     8041 acgagagatc ggcccgcgcg ctaccggcgc ggtacccgaa atgcgcgtcg ctccagacga
     8101 ggcgccctgc cagctgcctc gcataaggat cgacgaggag tttgtgcgca ttgaaacgca
     8161 atccgcgctc tggctgataa aggccgtgaa cgcggtaacc gtagagttgt cccggcgcga
     8221 catcgttgag gtagccgtgc cagacgtctt cggtgcgttc cggcagcgcg atgcgctcga
     8281 tctcgcgccg accatggctg tcgaataagc aaagctccac catttcggca ttggccgaaa
     8341 acagagcaaa attagttccg cgcccatccc atctggctcc caacggatgc ggcgcacctg
     8401 ccgaaatccg cacactaccg ctccggaacg agaaaaattg tcgcaagggg cggaacaaca
     8461 aggctgacct cgggaattgc gccctcgtcc aacgtggtga ccgcgcccgc gttcccgaca
     8521 ttgctgccgc cgtacagcgc ggaatcgctg ttgaggacct cgcgccaggt ccccgaaaat
     8581 ggcaccttga tgcgatagtc gcggtagatc tcaggggtga agttcaccac cacaaggcaa
     8641 cgctcgtgcg tctggcgtcc tttgcgcagc cacgcgaata cgctgcgttc ggcgtcatgc
     8701 atgaccagcc actcgaaccc ggcgtcctca caatcgagtt cgtgcagagc cggtatcgtc
     8761 cgataaagac gattgagatc acggaccaaa gcctgcacac ccgcatgccg ggcgtgtttg
     8821 agaagatgcc aatcaagcga atggtcatgg cgccattcgc tttcctggcc gaactcgcta
     8881 cccatgaaca gaagcttctt gccgggatgg ccgaacatga agccataata ggcgcgcagg
     8941 ttggcgaagc gctgccattc atcgcccggc attcggccaa gaatcgaacg cttgccgtgc
     9001 acgacctcgt catgggagag cggcaggatg aaattctcga agaacgcgta gtgcaagccg
     9061 aaaaggattt ggccgtgatg atgcttgcgg tagatcgggt ccttgctgat gtattcgagc
     9121 gtgtcgtgca tccagcccat gttccacttg tagccgaagc cgagaccgcc ccgatccacc
     9181 ggcctcgata ccatcggcca agccgtcgac tcctctgccg ccgtcgtggc attggggaat
     9241 cgtgagaata gctccgaatt gaagcggcga agaagattga tggcatcgag gttttcacgt
     9301 ccgccgtact tattcgggat ccagccgcct tcgggccggc tgtagtcgag atagagcatg
     9361 gacgcgaccg catccacgcg cagcccgtcg atgcggtagc gatcgagcca gaacaatccg
     9421 ctgcccagga ggaagtttgc gacttcggtg cgtccgtaat tgtagacgag cgtgttccaa
     9481 tcgaggtggc gaccttgctt cggattttca tgctcataca gtgccgtccc gtcgaaacgg
     9541 ctgaggccat gcggatcgtc ggggaagtgt cctgccaccc agtccacaat gacggcgagg
     9601 ccctttcggt ggcatccatc gatcaagacg gcaaagtcgg ctggagtgcc gaagcgactg
     9661 gtgggcgcga aaagtccggt cggctgatag ccccaggagc cgtcgaacgg atgttcgctg
     9721 gccggcagga attcgacatg ggtgaagccc atgtcgcccg cgtaggccgg caattcctcc
     9781 gccaactcgc ggtagctcag ccaacggccg ccatgttgcg catgccgccg ccacgatccg
     9841 agatggactt catagatcga tatcggcgcg cctaatgcat tgatgccgtc aggcgcgggt
     9901 tgcggccgtt ggatcgtctc gcggtcgacc acgatcgaag ccgtccgcgg tcgcagttcg
     9961 gccgcgaagg ccagtgggtc ggacttcaac ggcagcaggt ggccgtccgg tccgacgatc
    10021 tcatatttgt atttgtcgcc ggctcgggct tcgggcacaa aaatttccca aaagccgttg
    10081 ccgcgcacgc gcat
//
LOCUS       DATLJT010000194         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3914869,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000194 DATLJT010000000
VERSION     DATLJT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914869"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VM910_04440"
     CDS             complement(<1..217)
                     /locus_tag="VM910_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61831.1"
                     /translation="MPAVATGRELSGRRTTEEYLLHSRLLAFMARRHAIVLELCKAHL
                     QAGLHTRNATKTRPPRTISHASPLAATP"
     gene            323..1588
                     /locus_tag="VM910_04445"
     CDS             323..1588
                     /locus_tag="VM910_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI61832.1"
                     /translation="MKGCKVEIALKARPRMSLLAAAVTLSGAFFSAQAQEPIKIGFGV
                     SLTGGLASSGKAHLMSKQIWAEEINAKGGLLGRPIKLVYYDDQTNAATVPGIYAKLID
                     VDKVDLLMGHATNLIVAAMPLIIERKKQVMVLLALGSNAEFKYPRYFQSAAFGPDSKG
                     VLSNNFFAVAKSLAPEPKTVALVGADAEFSNNVLIGARENAKKYGLKIVYDRSYPPAT
                     VDYTPIMRAIQATSPDVVLVASYPPDSVGIVRAATEIGLKTQLFGGAMVGMQYASLMQ
                     QLGEKLNRVVNYHLYVPSAKMNFPGIEAFLKKYQAQAKEAGTDPLGFYQPPFAYAAMQ
                     VLEQAIKATGSLNDDKLASYIHNNAFNTIVGEIRFNELGEWATARMLLVQFQNVQGSG
                     LDQYMTGHKQVILYPPEYKDGELEAPFAK"
     gene            1682..>2040
                     /locus_tag="VM910_04450"
     CDS             1682..>2040
                     /locus_tag="VM910_04450"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI61833.1"
                     /translation="MEITAAVVREKFGAFTIDRLELTDPRPDEALVRIVASGMCQTDL
                     HGRDGYYNTPYPAVYGHEGAGVVQAVGNSVTKFAPGDHVVISFPWCGTCPNCRRNMQS
                     HCMKGLDLKMRGTRADGS"
BASE COUNT          417 a          638 c          619 g          366 t
ORIGIN      
        1 ccggcgtcgc cgccaacggg ctcgcatgac tgatggtacg gggaggcctg gtcttcgtcg
       61 cgttgcgggt gtggaggccg gcctggaggt gcgccttgca caactcaaga acgatcgcat
      121 gccgccgcgc catgaaggca agcaggcgcg aatgcaaaag atattcttcg gttgtgcggc
      181 gcccactcaa ttcgcggccc gttgcaacgg cgggcacggt cccatctcgc atcctggctt
      241 tcgagcactc agcaaatcgc tagtatcgtt cgcatcgaac gcacggtgcg ttggctcgtt
      301 cgatgccccg ggggaggacg ctatgaaagg gtgcaaggtc gagatcgcgc tgaaagcacg
      361 gccgcgaatg tcgctactgg cggcagcggt tacattgtcc ggcgcgttct tttcggcgca
      421 ggcgcaggag ccgatcaaga tcggctttgg cgtatcgctc accggcgggc tcgcctcctc
      481 gggcaaggcc cacctgatgt ccaagcagat ctgggccgag gagatcaacg cgaagggcgg
      541 gctgctcggc cgcccgatca agctcgtcta ctacgacgat cagaccaatg ccgcgaccgt
      601 gcccggcatc tacgcaaagc tgattgacgt cgataaggtc gacctgctga tgggccacgc
      661 gaccaacctc atcgtcgcgg cgatgccgct catcatcgag cgcaagaagc aggtcatggt
      721 gcttctcgcg ctgggctcga atgcggagtt caagtatccg cgctatttcc agagtgcagc
      781 gttcggtccc gattccaagg gcgtgctgtc gaacaacttc ttcgcggtcg ccaaatcgct
      841 cgcgccggag ccgaagaccg tggccctggt cggcgccgat gccgagttct ccaacaatgt
      901 gctgatcggc gcgcgcgaga acgcgaaaaa gtacggtctc aaaatcgtgt acgaccgcag
      961 ctatccgccg gcgacggtcg actacacgcc gatcatgcgc gccatccagg cgaccagccc
     1021 cgacgtggtt ctggtcgcgt cctacccgcc tgattccgtc ggcatcgttc gtgcggcgac
     1081 cgaaatcggc ctgaagactc aattgttcgg tggggcaatg gtcggaatgc aatacgcgtc
     1141 gctcatgcag cagctgggcg aaaagctcaa ccgcgtcgtg aactaccatc tctatgtccc
     1201 tagtgccaaa atgaatttcc ccggcatcga agcgttcctg aagaagtacc aggcgcaagc
     1261 caaggaagcc ggcaccgacc cgctcggctt ctaccagccg cccttcgcct atgccgcgat
     1321 gcaggtgctg gagcaggcaa tcaaggcgac cggcagcctc aacgacgaca agctcgcgag
     1381 ctacattcac aacaacgcat tcaacacgat cgtgggcgaa atccgcttca acgaactcgg
     1441 cgaatgggcg acggcacgca tgctgctggt gcagtttcag aacgttcaag gcagcgggct
     1501 cgatcagtac atgaccggtc acaagcaggt catcctctat ccacccgagt acaaagatgg
     1561 cgaactggag gctccgttcg cgaaatagcg gatgatgggt cgcaatagcg cgcgttgaac
     1621 acccacccta cccgctgtcg gttacctgct gttcgccacc gaccaaccag tgatagctgt
     1681 catggaaatc accgccgccg tcgtgcgcga aaaattcggt gccttcacga tcgataggct
     1741 cgaactgacc gatccgcggc ccgacgaggc gctggtcagg atcgtggcga gcggcatgtg
     1801 ccagaccgac ctgcacggtc gcgacggcta ctacaacacc ccctatcccg cggtttacgg
     1861 ccacgaaggc gcgggcgtgg tgcaagccgt cggcaacagc gtgacgaaat tcgcaccggg
     1921 cgatcatgtc gtcatctcgt ttccgtggtg cggcacctgc ccgaattgtc gacgtaacat
     1981 gcagtcccat tgcatgaagg ggttggacct gaaaatgcgc ggcacgcgcg cggacggctc
//
LOCUS       DATLJT010000195         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_9174,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000195 DATLJT010000000
VERSION     DATLJT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9174"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VM910_04455"
     CDS             <1..204
                     /locus_tag="VM910_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVI61834.1"
                     /translation="KDIGTVWEQQLPHRVRDLAAFVEEDVGNRETLESQLSFAIKTAP
                     KLTIQGGTTEVLRGIIARGLGLR"
     gene            282..1082
                     /locus_tag="VM910_04460"
     CDS             282..1082
                     /locus_tag="VM910_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI61835.1"
                     /translation="MSNYTDIGVEKHDHVGLIEIRRPPLNFFDVSLINQIADALEEFD
                     RDIEIRASVLAAQGKAFCAGANFNDPARQEQEERAKSDPASNLPINHLYVQAVRIFRN
                     KKPFVAAVHGAAIGGGLGLAVSADFRVTCPEARFSANFTKLGFHPGFGLTTTLSELIG
                     KNNAELMFYTSRRVTGEEAYRWGLANELVPQDQVRSAAMRLAQEIAECSPLGLISTRA
                     TMRAGLAERVLAATNHELVEQNRLRGTDDFKEGVKAVAERRAARFKGR"
     gene            complement(1238..1999)
                     /locus_tag="VM910_04465"
     CDS             complement(1238..1999)
                     /locus_tag="VM910_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61836.1"
                     /translation="MSYMRLRQICLVAPQLEPVIADIAGIMGLAVCYRDGNVAKYGLV
                     NALMPVDTILLEVVAPFRDGTAAGRFLEKTGGRGGYMAIFACEDPDARAAQANAIGVR
                     TANVITHAPYHGVQLHPRDCRAAFIEFNHTDGSDDVLGPYPPAGPEWQKFIRKDVTRM
                     LTGVEMQSPDPLALAEHWGRIIGVAVTRSDAGDAELRLPNCRFRFVKGEAEIMSALEF
                     RVRDVAAVREAARGRGYSVSGNEFLLGGVSFRLVA"
BASE COUNT          450 a          714 c          704 g          407 t
ORIGIN      
        1 aaggacatcg gcacggtgtg ggaacagcaa ttgccgcacc gggtgcgtga tctcgccgcc
       61 tttgtcgagg aggacgtcgg caatcgcgag acgctggaga gccaactgtc gttcgcgatc
      121 aagaccgcgc cgaaactgac catccagggc ggcaccacgg aagttttgcg cggcatcatc
      181 gcacgcgggc tcgggctgcg ttgacatcat gaaacgagcg caagctgctg cggtggtcga
      241 agttcacagc ggcgtaacca aaccgttcca agggattccc aatgagcaac tacactgaca
      301 tcggcgtcga aaagcacgat cacgtcggcc tgatcgagat ccggcgtccg ccgctcaatt
      361 tcttcgacgt ttccctgatc aaccagatcg cggatgcgct ggaggagttc gatcgcgata
      421 tcgaaatccg cgcctccgtg cttgcggcgc agggcaaggc attctgcgcc ggcgcgaatt
      481 tcaacgatcc agcgcggcag gagcaggaag agcgcgccaa atccgatccg gcgtcgaacc
      541 tgccgatcaa tcatctctac gttcaggccg tgcggatctt ccgcaacaag aagccgttcg
      601 tcgcggcggt gcatggcgcg gcgatcggcg gcgggcttgg gcttgccgta tcggcggatt
      661 tccgcgtgac ctgccccgaa gcgcgcttct cggcgaattt caccaagctc ggctttcatc
      721 cgggcttcgg gctgaccacg acgctttccg aactgatcgg caagaacaat gccgagttga
      781 tgttctatac cagccgccgc gtcaccggcg aggaagccta tcgctggggc cttgccaacg
      841 aactggtgcc gcaggaccag gttcgctcgg ccgcgatgag acttgcgcag gagatcgccg
      901 aatgctcgcc gctcggcctg atctcgacgc gcgccaccat gcgcgccggg ctggccgagc
      961 gcgtgttggc cgcaaccaat catgagctgg tcgagcagaa caggctgcgc ggcacggacg
     1021 atttcaagga aggcgtgaaa gcggtcgccg aacgtcgcgc cgccaggttc aaggggcggt
     1081 gatcgtctcg tcgtcccggc ccagcgaacg aaatgagcgc agcgccggga cccataacca
     1141 caggcggcac tttgggggca cagcgtcagc caccacgctc gatcgaaaga ttccgcggta
     1201 tgggtccccg ctttcgcggg gacgacagcg gcggggtcta cgccaccagt ctgaaactga
     1261 ccccacccag caggaactca tttcctgaga cggaatagcc gcgcccacgc gcggcctcgc
     1321 gaacagccgc aacatcgcgc acgcgaaatt ccagcgcgct catgatctcg gcctcgcctt
     1381 tcacgaagcg gaagcggcaa ttgggaagtc tgagctcggc atcgccggcg tcgcttctcg
     1441 tgacggcaac cccgatgatc cggccccaat gttcggccag cgcgagcgga tcggggctct
     1501 gcatctccac gccagtcaac atccgcgtca cgtccttgcg gatgaatttt tgccactcag
     1561 gccctgcagg cggataaggg ccgagcacat cgtcgctgcc gtcggtgtgg ttgaactcga
     1621 tgaaggcggc gcggcaatcg cgcggatgta gctgcacgcc gtgatagggc gcatgcgtga
     1681 tcacgttcgc ggtgcgcacg ccgatggcgt tcgcctgtgc cgcccgcgcg tcaggatcct
     1741 cgcaggcgaa gatcgccatg taaccgccgc ggccgccggt tttttccaga aagcggccgg
     1801 ccgcggtgcc atcgcggaat ggcgcgacca cttccagtaa aatggtgtcg accggcatga
     1861 gcgcgttgac gaggccgtat ttggcgacgt tgccgtcgcg atagcatacc gcaagcccca
     1921 tgatgccggc gatgtcggca atcacgggct cgagctgcgg cgcgaccagg cagatctgcc
     1981 gcaaccgcat gtagctcatt ttgatccccc ttgagttggc aagtcggacg tctctatcga
     2041 ttccaacccc ccttgaaaag ggacgctttc agtcgaaaaa gaataataaa tcaaggtgaa
     2101 atgatggaac tccggtacgt ctgacactcc gcgtcccggc taggcagggc atggccaccg
     2161 ccgaccatcg attctggttt tcaaagtcac ggccgcttga tgcaaatcaa cgtggctctt
     2221 catctcacct gtgagctgac aagttaggca accggagttg gcggtatttc gttcc
//
LOCUS       DATLJT010000196         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3684981,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000196 DATLJT010000000
VERSION     DATLJT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684981"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1126
                     /locus_tag="VM910_04470"
     CDS             20..1126
                     /locus_tag="VM910_04470"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HVI61837.1"
                     /translation="MSFSLPIHANGILPDRMIAAMADSGLILPAYPFVESQIQPASLD
                     LRLGDIAYRVRASFLPGPDATVAERIDELKLHDISLADGAVLETNCVYIVPLLESLAL
                     PAEIVAAANPKSSTGRLDVFTRVIADGTRRFDMIGAGYHGPLYAEISPKTFPVLLREG
                     SRLSQVRFRTGDAVLSDGELGALHGKERLVDTDDADLTGGVALSVDLSGANSGGFVGY
                     RAKRHTGVVDVDRRGGYPVEDFWEAISARSDGSLILDPGEFYILASKEAVQVPPDYAA
                     EMVPFDPLVGEFRVHYAGFFDPGFGYAGAGGKGARAVLEVRSREVPFILEHGQIVGRL
                     VYEKMLARPAALYGQRIGSNYQAQGLKLSKHFRV"
     gene            1312..2364
                     /locus_tag="VM910_04475"
     CDS             1312..2364
                     /locus_tag="VM910_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61838.1"
                     /translation="MNERADAAEQALWLKWRSVADLYHAYFTGIILTVVTRRGTADAA
                     EFVFRVFRRQQQERFLPGLKKLGIDHLPPAVAAAQYHYLSNWIGGVHVEYMYESERKA
                     WIRYPPPRWIWKGTAICGIPSDVSKAMLRGWHANNGVALGNLKLGFVCTKQSVDGQDG
                     LEGYYYEYDRPLELDQRLVFARHQEAPMFDRASAPALPVSSWPRPRLEKAYRNYAMEY
                     VKTAAPVMVQLFGPEEAGHLLRLTGKLIGMQYFDEVAQALSQARGGANEFAGFVRALF
                     DAQDDVAEIVNSAASFEIRQQSWTLMADVADYHPACASVLEGLFEGLAAGCGRHIPVS
                     LKTASAGRPPFVWSVG"
     gene            2541..>2801
                     /locus_tag="VM910_04480"
     CDS             2541..>2801
                     /locus_tag="VM910_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVI61839.1"
                     /translation="MSDISVAEIPVDEDERPLPPPRPPVPNVLVDGPILPTLLRLAWP
                     NVIALTAGTCVVIAETSYIGRLGVESLAAMALVSPFVILIMTM"
BASE COUNT          465 a          885 c          917 g          534 t
ORIGIN      
        1 gccactcaag gaccatagcg tgtcattttc gctccctatc catgccaatg gcattttgcc
       61 cgatcgcatg atcgcggcga tggcggatag cggcctgatc ctgccggcct atccctttgt
      121 cgaaagccag attcagccgg cgagccttga cctgcggctc ggcgatatcg cctaccgggt
      181 ccgcgccagc ttcctgcccg gccccgatgc gacggtcgcc gagcgcatcg atgaattgaa
      241 gctgcatgat atctcgctgg ccgacggcgc ggtgctggag accaactgcg tctatatcgt
      301 gccgctgctg gagagcctcg cgctgccggc ggagatcgtg gccgccgcca acccgaagag
      361 ctcgaccggg cggctggatg tcttcacccg cgtgatcgcc gacggcacgc gcagattcga
      421 catgatcggc gccggctatc acggcccgct ctatgccgag atcagcccga agacgttccc
      481 ggtgctcttg cgcgagggct ccaggctgtc gcaagtgcga ttccgcaccg gcgatgccgt
      541 cctctcggac ggcgagctcg gcgcgctgca tggcaaggag cggctggtcg acactgatga
      601 tgcggatttg accggcggcg tcgcgctgtc cgtcgatctc tccggcgcca actccggcgg
      661 ctttgtcggc tatcgcgcca agcgccacac cggcgtggtc gatgtcgatc gccgcggcgg
      721 ctaccctgtc gaggatttct gggaggcgat cagcgcgcgc agcgacggca gcctcatcct
      781 cgatcccggc gagttctata tcctggcctc gaaagaagcg gtgcaggtgc cgccggatta
      841 tgccgccgaa atggtgccgt tcgatccgct ggtcggcgaa ttccgcgtgc actatgccgg
      901 cttcttcgat cccggtttcg gctatgccgg tgccggcggc aagggcgcgc gcgccgtgct
      961 ggaagtacgc tcgcgcgagg tgccgttcat cctcgagcac ggccagatcg tcggccggct
     1021 tgtctacgaa aagatgctgg cgcggccggc cgcactctac ggccagcgca tcggctcgaa
     1081 ctaccaggcg cagggcctga agctctccaa acattttcgg gtatgaatta ttctgtcgtc
     1141 cggacaggcg aggcggagcc gtgcggtccg cgacctatac cgcggaatcc atcgttgaag
     1201 taggatgacc gagatctttc gcaaaaccag cgcttgtggt tatggatacc ggcttgcgct
     1261 ggcgttgcgc gccgggacga cagctacaaa cgatgcctgt gaggaaatgc aatgaatgaa
     1321 agggcggatg ccgcggagca ggcgctatgg ctcaaatggc gcagcgtcgc cgatctctat
     1381 catgcctatt tcaccggcat cattctgact gtggtcacgc ggcgaggcac ggctgatgcg
     1441 gccgagtttg tcttccgtgt gtttcgtcgc caacaacagg agcgttttct gccgggcttg
     1501 aagaaactcg gcatcgatca tttgccgccg gcggtcgcag ccgcgcagta ccattatctc
     1561 tcgaactgga ttggcggcgt gcatgtcgaa tacatgtatg agagcgagcg caaggcctgg
     1621 atccgctatc cgccgccgcg ctggatctgg aagggcacgg cgatctgcgg cattccttcc
     1681 gacgtttcga aggcgatgct gcgcggctgg cacgccaaca acggcgtggc actgggcaat
     1741 ctcaagctcg gattcgtctg caccaagcag agtgtcgacg gccaggacgg tctcgaaggc
     1801 tattactacg aatacgatcg tccgctcgag ctcgaccagc gcctggtctt cgcgcggcat
     1861 caggaggcgc cgatgttcga tcgcgcgagc gcgccggcct tgcccgtttc gagctggccg
     1921 cggccgcggc tggaaaaggc ctaccgcaac tatgcgatgg aatatgtcaa gacggccgcg
     1981 cccgtgatgg tgcaattgtt cggacccgag gaggcgggcc acctgctgcg tctcaccggc
     2041 aagctgatcg gcatgcaata tttcgacgag gtcgcgcagg cgctctcgca agcccgcggc
     2101 ggcgcgaacg agtttgcggg cttcgtgcgt gcgctgttcg atgcgcagga tgacgtcgcc
     2161 gagatcgtga actccgctgc ttccttcgaa atccgccagc agagctggac gctgatggct
     2221 gacgtcgccg attaccaccc cgcctgcgca agcgtgctcg aaggcttgtt cgagggcctt
     2281 gccgccggct gcggccggca cattccggtg agcctgaaga ccgcgtcggc cggcaggccg
     2341 ccgttcgtct ggtcggtggg ctaggagagg ggatgcgcga cccgcattcc cgacaggtcc
     2401 ccaatgcgct tggcgcaggc gggaggcatg tccgttcgtg ctaagcaacc tgtccggcga
     2461 ctcgcgggcc gagcaatcat tgaaatcgcg cagggtcgtg tcgccgaacc ggacttccgg
     2521 ttgatgcgcc gggagacgaa atgtccgata tcagcgtcgc cgaaattccc gtcgacgagg
     2581 acgagcggcc gcttccgccg ccgcggccgc ccgtgccgaa cgtgctggtc gacggtccga
     2641 tcctgccgac gctgctcagg ctcgcctggc ccaatgtgat cgcgctgacc gcgggcacct
     2701 gcgtggtgat cgcggagacc tcctatatcg gtcggctcgg cgtggaatcg cttgcagcga
     2761 tggcgctggt gtctcccttc gtgatcctga tcatgacgat g
//
LOCUS       DATLJT010000197         4204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4144959,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000197 DATLJT010000000
VERSION     DATLJT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4144959"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(757..3327)
                     /locus_tag="VM910_04485"
     CDS             complement(757..3327)
                     /locus_tag="VM910_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI61840.1"
                     /translation="MSASESRFEAVRVAGLTDLIGREDELDFLLERRRLAWKGEGQIV
                     LISGEPGIGKSRLAAALAERIAGEPHTRLRYQCSPYHTNSALRPFIAQLERAAAFKAD
                     DAPEQRLDKLEALLAMGASRVEAVAPLFAALLSIPFGKRYAPLALSPTQQRRRTLAAL
                     LDQFEGLARRQPILLSFEDAQWADATSLELLDLTVERVRQLPVLALFTFRPEFEPPWA
                     GLPNVGTLTLGRLDRDDVENMVARVTGGRVLPAEVMKQIVAKTDGNPLFVEELTKTVL
                     EAGILVEDADGYRLDRPLPPLAIPATLQDSLMARLDRRAPVREIAQIGAAIGREFSYS
                     LLRALVGGDESALKHGLAQLEQAELVFRRGEPPDAGYSFKHVLVRDAAYESLLKSRRQ
                     QLHGQIARALEESFADIAASQPEIVAHHFTEAGLAEPAIDYWLKAGQQAARRSANAEA
                     LNHLARGLELLPNIDDPMLRNKSELLLQTSLGHSLRSIKGWSIDSVKHAYTRALQLCK
                     ESGFDEHTLPAVFGLWTWNFLRAALGEAQALAEHLVNTAENADDSVFRVLAHEALGFT
                     LFARGKFVAAHAALERSISMCEDSKATAYLDLSAQDPRVHVRLYDGIVLWLLGYPDQA
                     LRICADARRYAETSQHPFSDALARTISLRVHQLRGEAAVVAGQADAAIALCEEHEFVH
                     YLAMALILRGWASAQQGEFEKGIAEIQAGLEKERATDALLFESYTLGLLADACIKNER
                     YGQAFDFLHQAQSRLDEENSERYYAAEIYRLLGETYLRSRQDLDQAERWLCKGLTVAR
                     EQKAKSLELKLCLSMYDVYELRHNADAYRPQLGEIYGSFSEGFDTTDLVRAKARLKNA
                     "
     gene            3376..4080
                     /locus_tag="VM910_04490"
     CDS             3376..4080
                     /locus_tag="VM910_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61841.1"
                     /translation="MPAEIAQTEEIAEQPAGGGGDDDRSGLSQGLKAGCKVRRLSDHR
                     VLAQRTLAAEVADHHDAARDANADRERFRGARLELSNGGDDIEPRPHGSLGIVFVRDW
                     IAEISQYPVAPELGEEAVIGSRDTGAGGVIGIDHSAHVLRIEAGRQRRRANQIADHHG
                     EVTAFGLVTRHRFGRCFSRCRSGWKLCDRAQNLAAMPQQNAEILEVLLGQIADDREVD
                     GVVGEPLGVLTQADRC"
BASE COUNT          801 a         1343 c         1258 g          802 t
ORIGIN      
        1 tcggtaacgc cgcgcgcaac aacctcgcgg ccgtccttga tgacttccca ataccagtgg
       61 ccatcttcgc cattgggcat gatgcggaat tcaacaccgt ttcccattgt aggttgggtc
      121 cttcggcccg aaatgattat ccgggtagat caggacactc tctactgcgc cgcactcaga
      181 gcacacgaag gcgcgaaggc ccggctcggg gtcccgagga gaaatatcag ccactatttc
      241 catcctcccg ccgcaccgct tgcaggccgg atctgacaac gccaccattt ctcttgccca
      301 ttaccactca cgctcacaac gcatagatgg gccggacggt tccgcgggag gttccctggg
      361 attcggcacg tgactgctgc ttcggcgcag cttatgcgcc agttctcgcc actcgtcttc
      421 ggccacggcg cgctaaaaag gaaagccccg gccgcaagca tcgggcgcga ggcgggggcg
      481 ggatattttg cgttcgcgag tcaggaccta gcgctgcggt tggcgctctc gcaggtctgt
      541 tagctccgtc tccagtgggc cgcacttaaa acggcatggg ctagggctca cgcgcagaac
      601 tagttagccc caccaacgtc aagcttggcg tcgtagaaca gcgctactca aattgcctgc
      661 tgaaaacgtc gacatcggcc tctgccagta gcaattcggc cgcgccttgc cattgtgcag
      721 tcgtctctcg gtgttttggg ctgccgccta ttctaatcaa gcatttttca gccttgcttt
      781 ggctctgacg agatccgtgg tgtcgaaacc ctcgctaaag gatccataga tctcgccgag
      841 ttgcgggcga tacgcgtcgg cgttgtgtct tagctcgtac acgtcataca tgctcaggca
      901 aagtttaagt tcaagggatt tggctttctg ttcgcgcgcg acggtaaggc ccttacacaa
      961 ccaacgctct gcctgatcca gatcttgacg cgatcgcagg tatgtttcac caagcaagcg
     1021 atatatttca gccgcgtaat agcgttccga attctcttca tcgagtctcg attgcgcctg
     1081 gtgcaaaaaa tcgaacgctt gcccgtaacg ttcattcttt atgcatgcat ccgccagcaa
     1141 ccccagagta tacgactcaa acaataaggc gtccgtggcc cgttcttttt ccaaacctgc
     1201 ctgaatctcg gcaatgcctt tctcaaattc gccttgctgg gcacttgccc atccacgcaa
     1261 aatcaaagcc atggccagat agtgcacaaa ctcatgctct tcgcagagcg cgattgccgc
     1321 gtcagcctgg cctgcgacaa cggcagcctc gccgcgcaac tgatgcaccc gcaggcttat
     1381 cgttcgtgcc aatgcgtcgc tgaacggatg ttgagatgtc tctgcataac ggcgagcgtc
     1441 ggcgcaaatc cgcagcgcct gatcgggata gccgagcaac cacagtacta tgccatcgta
     1501 taaccttacg tgaactcgcg ggtcttgcgc tgacaggtcg agatacgccg tggccttgct
     1561 gtcctcacac atgctaatgc tgcgttctag cgccgcgtgc gcagcgacaa atttcccgcg
     1621 cgcaaataac gtaaacccta gagcttcgtg ggcaagaacc ctgaaaaccg agtcatctgc
     1681 attctcggct gtattcacca aatgttcggc aagggcttga gcctcaccca gcgctgcacg
     1741 aaggaaattc caggtccaca gaccaaacac cgcaggcaag gtatgttcgt caaacccgct
     1801 ctctttgcac agctggagtg ctcgcgtata cgcatgcttc acactgtcaa tgctccagcc
     1861 cttaatcgac cgtagagagt ggccgagcga cgtttgaagc agcaactccg acttgttgcg
     1921 tagcatcggg tcgtcgatat tgggcaggag ctccaaacca cgagcaagat ggtttagtgc
     1981 ctcggcattg gccgagcgac gggctgcctg ctgtccggcc ttgagccagt aatcgatggc
     2041 gggttcagca aggccggctt ccgtgaagtg gtgcgccacg atttccggct ggctcgccgc
     2101 gatgtcggca aagctctcct cgagtgcgcg cgcaatctgg ccgtgcaatt gctgccggcg
     2161 gcttttgagc aggctctcat aggccgcatc ccgcaccagc acgtgtttga agctgtagcc
     2221 ggcgtccggc ggctcgcccc ggcggaacac gagctccgcc tgctcgagtt gggccagtcc
     2281 atgcttcaat gcgctctcgt cgccgccgac caacgcgcgc agcaaagaat aggagaactc
     2341 gcgcccgatc gccgcgccga tttgggcgat ctccctcacc ggcgcccggc ggtcgagccg
     2401 cgccatcaaa gagtcttgaa gcgtcgccgg gatggcgagc ggcggcaggg gccggtcgag
     2461 ccgatagccg tcggcgtcct cgaccaggat gcccgcctcc agcaccgtct tggtgagctc
     2521 ctcgacgaac agcgggtttc cgtcggtctt ggcgacgatc tgtttcatca cttcggcagg
     2581 cagcacgcgc cccccggtca cccgcgcgac catgttttca acgtcgtcgc ggtcgaggcg
     2641 accgagcgtc aaggtgccga cgttggggag accagcccag ggcggctcga actccggtcg
     2701 gaaggtgaac agtgccaaca ccggcagttg gcgcacccgc tcgaccgtca gatcgaggag
     2761 ctcgagcgag gtggcgtccg cccattgcgc atcctcgaac gaaagcaaga tcggctgccg
     2821 gcgggcgaga ccctcgaatt gatcgagcag tgccgcgagc gtccggcggc gttgctgcgt
     2881 ggggctgagc gccagcggtg catatcgttt gccgaacggg atcgagagca gcgccgcaaa
     2941 aagcggcgct acagcttcga cccgcgaggc gcccatggcg aggagcgctt ccaacttgtc
     3001 gaggcgttgc tcgggcgcat catctgcctt gaacgccgcc gctcgctcca actgggcgat
     3061 gaacggacga agcgcgctgt tggtatggta gggcgagcat tggtaacgca agcgcgtgtg
     3121 cggctcgccg gcgatgcgct cggcgagagc tgccgcaagg cgggacttgc cgatccccgg
     3181 ctcgcccgag atcagcacga tctgcccctc gcccttccag gccaggcgtc ggcgctccag
     3241 caggaaatcg agctcgtcct cgcgaccgat gagatcagtc aggcccgcca cgcggaccgc
     3301 ctcgaagcga ctctcggaag ccgacacgcc ttcgaccgcc caggccgcga ccggctcggc
     3361 aatgcccttg acctcgtgcc ggccgagatc gcgcagacgg aagagatcgc cgagcagccg
     3421 gcgggtggag gcggcgacga cgatcgttcc gggctcagcc aaggcctgaa ggcgggctgc
     3481 aaggttcggc gtctctccga ccaccgcgtg cttgcgcaac gcaccctcgc cgccgaagtc
     3541 gccgatcacc acgacgccgc tcgcgatgcc aatgcggacc gcgagcggtt ccgcggcgcg
     3601 cgtctcgagt tgagcaacgg cggcgatgat atcgagcccc gcccgcacgg ttcgctcggc
     3661 atcgtcttcg tgcgcgattg gatagccgaa ataagccaat atcccgtcgc cccggaactc
     3721 ggcgaggaag ccgtcatagg ttcgcgtgat acgggcgcag gcggcgtgat aggcatcgat
     3781 cacagcgcgc atgtcctccg gatcgaggcg ggccgacagc gccgtcgagc caaccagatc
     3841 gcagatcatc acggtgaggt gacggcgttc ggcctcgtca cgcgccaccg gttcggtcgc
     3901 tgtttcagtc gatgcaggag cggctggaag ctctgcgatc gcgcgcagaa tcttgcggcg
     3961 atgccccagc agaacgccga gatccttgag gtcttgctcg gtcagatcgc ggatgaccga
     4021 gaggtcgatg gcgttgtcgg cgaaccgttg ggcgtactca ctcaggccga tcgatgctag
     4081 ccactcggca atgcctttca tggcacgtcc cgtcgatggc gtgccgcatc atacgcgcat
     4141 agtgttccgg cgccatcgcg caatgcttga gtctggagca ggagttccac cacacggcgt
     4201 cgaa
//
LOCUS       DATLJT010000198         7693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3455787,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000198 DATLJT010000000
VERSION     DATLJT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455787"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VM910_04495"
     CDS             complement(<1..413)
                     /locus_tag="VM910_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI61842.1"
                     /translation="MSESPDLVIRGGTVADGRGGDLFEADVAIRDGRISEVGKISGKG
                     RDEIDARGKLVAPGFVDIHTHYDGQVTWSQDMTPSSQNGVTTAVMGNCGVGFAPCRPD
                     DHLRLIELMEGVEDIPEPVLSAGIPWSWESFPDYM"
     gene            complement(508..1746)
                     /locus_tag="VM910_04500"
     CDS             complement(508..1746)
                     /locus_tag="VM910_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61843.1"
                     /translation="MADVLAAPQQAKTDTTFRTLSLISTAHWVSHFHMLVLPMLFPFL
                     RAQLGVGYIELGFALTVFAVVSGLTQAPLGYIVDHVGARKILLAGLCLGGVALIMLGL
                     HLSYTWLVASAGLLGVANSVYHPANYAILSAHMDDAWMGRAFSVHTFAGFLGGAVAPA
                     IMAALVTWIGGFGALIVAGAVGPLVAALLVTLGVPDAGAKHRRASGAATPQQSIVTPL
                     LIMLTIFFTLLGLSSAGIGNFGVVALMSGYGASFSTANVALTVYLGASAVGVLAGGVL
                     ADRTKRHGQVSAACFAVNAAVVFLIACTTPPTVLLIAAMMVAGFLGGVIAPSRDMMVL
                     NAAPAHAAGRAFGIVSTGFNFSGIASPLLFGWIMDQNMPRWVFGASAAFMVLTVLLAF
                     FTDRDRREQEEEPDLGLMSP"
     gene            complement(1894..2055)
                     /locus_tag="VM910_04505"
     CDS             complement(1894..2055)
                     /locus_tag="VM910_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61844.1"
                     /translation="MSYGGRKKEQPKQADDKSKKRPAAPSDDETEDYEDGDIATPKRD
                     RYGTDDEPL"
     gene            complement(2084..3124)
                     /locus_tag="VM910_04510"
     CDS             complement(2084..3124)
                     /locus_tag="VM910_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI61845.1"
                     /translation="MIFRQLFDSVSGTYSYLLASRLGGEALILDPVLEKADRYCQLLR
                     ELDLKLVKAVDTHLHADHVTGLGELRDRTHCITIMGEQSKADVVSMRVSDGDKVTIEG
                     LCLDVMYTPGHTDDSYSYLMGDRVFTGDTLLIRGTGRTDFQNGSARAQYESIFNRLLK
                     LPEETLVYPAHDYKGDTVSTIGEEKRYNPRLQVRSVDEYIELMGNLKLPNPKMMDVAV
                     PANMHVGLHQEDLEKQGLSLSASQAIASLGRPDILLVDLRESGERAKHGTLEGALHAP
                     YPSIGENLRPGGMLREVAAATGRRIVFFCAFGERSAMAVTAAKDAGLANTAHITGGLD
                     AWKKAGGPVVRN"
     gene            complement(3326..3772)
                     /locus_tag="VM910_04515"
     CDS             complement(3326..3772)
                     /locus_tag="VM910_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HVI61846.1"
                     /translation="MFRRFAVAALLSIASAVPGLAAEGKTHRIAIQVDQNDPQVMTMA
                     LNNATNVIEFYRTMNEDVDIEVITYGPGLHMLRADTSPVQDRIKHLKEMVFPGRLQFS
                     ACNNTKLGMEKTEGRAISVVPEATVVPSGVVRLMELQEQGWSYVRP"
     gene            complement(3853..5592)
                     /gene="soxB"
                     /locus_tag="VM910_04520"
     CDS             complement(3853..5592)
                     /gene="soxB"
                     /locus_tag="VM910_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HVI61847.1"
                     /translation="MKIRRRDFLKTTGAALLLGGARHARAAETPSLYDLERFGNARIL
                     HLTDVHAQLRPVYFREASVNIGIGEMLGRPPHLVGKAFLDRFGISADSAEAYAFTSLD
                     FEKSAQRFGKLGGFAHLKTLIDRLRNDVGEGHSLLLDGGDLWQGTGLANSMLGADMVE
                     AANLLGIEAMTGHWEFTYGEAALRANLERFKGEFLAQNVFLTEEAAFNDAKAFDPASG
                     RVFKPATIKEIGGHRVAVIGQAFPYVPIAHPKRFTPNWTFGIRDEEMQKLVATLRDKD
                     KVDCVLLLSHNGMDVDLKLASRINGIDVILGGHTHDAVPQPVAVKNAGGTTLVTSAGS
                     NGKFLAVLDLDLAKGKVGGVRYRLLPVYSELLKPDAAMKALVEQLHASHSKSWSDTIA
                     NADRLLYRRGNFSGPMDQMICDALRRELDCEIALSPGFRWGTTSLAGQPITMEDVLSE
                     TAITYPETYAQIMTGSQIKDVLEDVCDNLFNPDPYYQQGGDMVRVGGLSYTCSPNESI
                     GKRISDLKLDSGSPLQAGKSYKVAGWASVNEQKGAPVWDVVANYLRAAKLPAMRTSRV
                     NLQGVDGNPGIAG"
     gene            complement(5614..5934)
                     /locus_tag="VM910_04525"
     CDS             complement(5614..5934)
                     /locus_tag="VM910_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61848.1"
                     /translation="MRILSNGWNMTLAAVLLIAGTMPARAASEADFKAAYAAAEAAEK
                     EASALHNQWTTTEATLAAAKKSASAGDFDAALAAAKEAEALAKASIFQATSEKERWKD
                     AVIR"
     gene            complement(5931..6785)
                     /gene="soxA"
                     /locus_tag="VM910_04530"
     CDS             complement(5931..6785)
                     /gene="soxA"
                     /locus_tag="VM910_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HVI61849.1"
                     /translation="MRVPLLLQSALATLALAIFIHAAAAADTPDPAGDAKAFRDYFVR
                     KFPQVKLEDFANGPYSMNADMRRQWDEKEQFPPYEFALETGKEMFATPFRNGKTYADC
                     FPNGGIGIRQNYPYFDEKEGKVVTLELALNRCREANGEAPYSYVKDEMASLTAYMAFT
                     SRRKPMDIKVPDDPRALAAYEDGKRYFYTRRGQLNFSCASCHVQSPGERIRAETLAPA
                     LGIMNALPIYRSEWSGMGTTSRRLVTCNSQTRAVPLDPQSDEYRDLEYFLSYMSNGLP
                     ISGPGARP"
     gene            complement(6788..7099)
                     /gene="soxZ"
                     /locus_tag="VM910_04535"
     CDS             complement(6788..7099)
                     /gene="soxZ"
                     /locus_tag="VM910_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HVI61850.1"
                     /translation="MASTIRVRAISNGDVTEVQALIQHPMDTGLVKDAKGDLIPAHFI
                     QQLTFEHNGKTVFLADWGPAVARDPYVKFSFKGAAKGDDLKIAWVDNTGATDTMTAKI
                     Q"
     gene            complement(7174..7665)
                     /gene="soxY"
                     /locus_tag="VM910_04540"
     CDS             complement(7174..7665)
                     /gene="soxY"
                     /locus_tag="VM910_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HVI61851.1"
                     /translation="MTTVDSLPLSRRLVLKGAGAVALIGLGNLPLLPMPAIAAANDKY
                     PENAFKQKSDADAIKALYGRTAESSDKVKLDAPEIAENGSVVPVSVTTTLAGVTSISF
                     FVSENPNALAASYAIPEGTLPNVANRLKMAKTCNVIAIVESEGKLFSATKEVKVTVGG
                     CGG"
BASE COUNT         1408 a         2384 c         2382 g         1519 t
ORIGIN      
        1 tccatgtagt ccggaaagct ttcccaggac caggggatgc cggcgctcag caccggctcg
       61 gggatgtcct cgacgccctc catcaactcg atcaggcgca gatggtcgtc cggccggcat
      121 ggcgcgaagc cgacgccgca attgcccatc accgcggtcg tgacgccgtt ctgcgaggat
      181 ggcgtcatgt cctgactcca ggtgacctgg ccgtcgtaat gcgtgtggat gtcgacaaag
      241 cccggcgcga ccagcttgcc gcgcgcgtcg atttcgtcgc ggcccttgcc cgaaatctta
      301 ccgacctcgc tgatccgtcc gtccctgatc gcgacatcgg cctcgaacag atcgccgccg
      361 cgtccatcgg caacggtacc gccgcggatc acaaggtctg gactttcgct catggcgttt
      421 catcccgagt tttttttggg tcgttgaagc ctatgtttgg cgcatgaaaa cctggtcccg
      481 ttttccctcg cgccaatgct tgcggcatca gggcgacatc aagccaagat caggttcctc
      541 ttcctgttcg cgccgatcgc ggtcggtgaa gaaggcaagc agcacggtaa gcaccatgaa
      601 cgcggcggac gcgccaaaca cccaacgcgg catgttctgg tccatgatcc agccgaacag
      661 cagcgggctc gcgatgccgc tgaaattgaa accggtcgag acgatgccga aggcgcgtcc
      721 ggcggcgtgc gcgggtgcgg cgttgagcac catcatgtcg cgcgaggggg cgatcacgcc
      781 gccgaggaat ccggcgacca tcatggctgc gatgagcaac acggtcggcg gcgtcgtgca
      841 ggcgatcagg aacaccacgg ccgcgttgac cgcgaagcaa gcggcggaaa cctggccgtg
      901 acgcttcgtg cgatcggcga gaacgccgcc ggccagcacg cccaccgcgc tcgcgccgag
      961 atagaccgtc agcgcgacgt tggcggtcga gaacgaggcg ccgtagccgc tcatcagcgc
     1021 gaccacgcca aaattgccga tgccggcact ggacaggccg agcagcgtga agaaaatcgt
     1081 cagcatgata agaagcggcg tgacgatgct ttgttgtgga gttgccgcgc cgctggcacg
     1141 ccggtgtttt gcgcccgcgt cgggcacgcc caatgtaacc agaagcgccg cgacaagcgg
     1201 cccgacggcg ccggccacga tcagcgcgcc gaacccgccg atccacgtga ccagcgcggc
     1261 catgatcgcg ggcgccaccg cgccgccgag gaagccggcg aaggtgtgga ccgagaaggc
     1321 gcggcccatc cacgcgtcgt ccatatgtgc cgagaggatc gcgtaattgg ccggatgata
     1381 gacgctgttg gcgacgccga gcagtcccgc acttgcgacc agccatgtgt agctgagatg
     1441 cagaccgagc atgatgagcg cgacgccgcc gaggcaaagc ccggccagca gaattttacg
     1501 cgcgccgaca tgatctacga tgtagccgag cggcgcctgc gtgaggccgg atactacggc
     1561 gaacacggtc agtgcaaagc cgagctcgat atagccgacg ccgagctgtg cccgcaggaa
     1621 cggaaacagc atcggcaata ccagcatatg gaaatggctt acccagtgcg ccgtcgagat
     1681 cagggacagc gtgcgaaagg tcgtgtcggt tttggcttgt tgcggtgcgg cgagaacgtc
     1741 ggccatcgtt aactagcgga attccatgat ctctttgact tcagcgaact atcggcgaca
     1801 ggcggtgatt tgtccatcga cagcgctgca tggctcttgt ccgtttccga cttcccgttc
     1861 cgcctgtgcc gctgcgtcgt cgctctgcag catctacagc ggctcgtcgt cggtgccgta
     1921 gcgatcccgc ttgggggtag cgatatcgcc gtcctcgtag tcctcagtct cgtcatcgct
     1981 tggcgcagcc ggccgcttct tcgacttgtc gtcggcctgc tttggctgct ctttcttcct
     2041 gccaccgtaa ctcatgtgat cccccagtga gtgcagttgc ggatcagttg cgaacgaccg
     2101 gtccgccggc tttcttccag gcgtccagtc cgccggtgat atgcgcggta ttggcgaggc
     2161 ctgcgtcctt tgcggccgtg accgccatgg ccgagcgttc gccgaatgcg cagaagaaca
     2221 cgatgcgtcg tccggttgcc gcggccacct cgcgcagcat gccgccaggc ctgaggttct
     2281 cgccgataga ggggtaaggc gcatgcaagg cgccctcgag cgtgccgtgc tttgcgcgct
     2341 cgccgctctc gcgcaaatca accagcagga tgtccgggcg gccgaggctt gcgatcgcct
     2401 gggacgcgga aagcgaaagc ccctgcttct cgagatcctc ctgatgcagg cccacatgca
     2461 tgttggcggg aacggctacg tccatcatct tcgggttggg caatttgaga ttgcccatca
     2521 gctcgatgta ttcgtcgacc gagcgcacct gcagccttgg attgtagcgt ttctcttccc
     2581 cgatcgtgga gacggtgtcg cccttgtaat cgtgggcggg atacaccagc gtttcttcgg
     2641 ggagtttcag cagccggttg aagatggatt catactgcgc ccgcgcgctg ccgttctgga
     2701 aatcggtgcg gccggtgccg cggatcagga gcgtgtcgcc ggtgaagaca cggtcgccca
     2761 tcaggtaact ataggaatcg tcggtatggc cgggcgtgta catcacgtcg aggcacaggc
     2821 cttcgatcgt caccttgtcg ccgtcggaca cccgcatcga caccacgtcg gccttgctct
     2881 gctcgcccat gatggtgatg caatgggtgc ggtcgcgtag ttcaccgaga cccgtcacat
     2941 ggtcggcgtg cagatgggtg tcgacggcct ttaccagctt gagatcgagc tcgcgcagca
     3001 actggcagta acgatctgcc ttttccagca cgggatcgag gatcagggct tcgccgccga
     3061 ggcggctcgc gagcagatag ctgtaggtac cggaaacgct gtcgaagagc tgacggaaga
     3121 tcatgatcgg ttaccgctgt aatgatttct ttgatcccag tgttccgatt ccggaattcg
     3181 catcatgatg agggtgcttc gtttgcgaac ttcggaaatc gggaccctaa caacatattg
     3241 atccagtgtg gcctctgctt ggaaatccgc aagcgaacgc gatgccaaaa aattaccagg
     3301 acttctaaat ctccacacta gcacatcacg gccggacata gctccagccc tgctcctgca
     3361 attccatcag gcgcaccacg ccggagggga cgaccgttgc ctccggcacc acggaaatcg
     3421 cgcggccctc ggtcttctcc atgcccagct ttgtgttgtt gcaggccgag aactggagcc
     3481 tgcccgggaa caccatttcc ttgaggtgct tgatgcgatc ctgcaccggc gaagtgtcgg
     3541 cgcgcagcat gtgcagcccc ggaccatagg taatgacctc gatgtcgacg tcctcgttca
     3601 tggtgcggta aaactcgatg acattggtgg cgttgttcag cgccatggtc atgacctgcg
     3661 gatcgttctg atcgacctgg atcgcgatgc gatgcgtctt gccctcggcg gcgaggccgg
     3721 gaacagcgga ggcgatcgac agcagtgctg cgactgcgaa gcggcgaaac atcagaatct
     3781 ccgtttgggt ttgcgcttcg cgcggccgga ttgaagacga gcaatatacc ccttttgatc
     3841 ggcgatatat cactatcccg cgatgcccgg attgccatcg acgccttgca aattcacccg
     3901 gcttgtgcgc atcgccggca gttttgccgc acggaggtag tttgccacca catcccagac
     3961 cggcgcgccc ttttgctcgt tgacggaggc ccagcccgcc accttgtaac tcttgccggc
     4021 ctgcagcggc gagccgctgt cgagcttcag atcggaaatc cgtttgccga tgctctcgtt
     4081 tggtgagcag gtgtaggaga gaccgccgac gcgcaccatg tcgccgccct gctgataata
     4141 tggatcgggg ttgaacaaat tgtcgcagac gtcctccagc acgtccttga tctggctgcc
     4201 ggtcattatc tgcgcatagg tctcgggata ggtgatcgcg gtttccgaca gcacatcctc
     4261 catggtgatc ggctggccgg caagcgacgt cgtgccccag cggaagcccg gcgacagcgc
     4321 gatctcgcaa tcgagttcgc ggcgcagggc gtcgcagatc atctggtcca tcgggccgga
     4381 aaagttgccg cggcggtaaa gcaggcgatc ggcgtttgcg atcgtgtcgc tccagctctt
     4441 cgaatgcgac gcgtgcagtt gttcgaccag cgccttcatc gccgcatccg gcttcaacag
     4501 ttcggaatag accggcagca ggcgatagcg cacgccgccg accttgcctt tggcaaggtc
     4561 gaggtcgagc acggccagaa acttgccgtt cgaaccggcg ctggtaacga gcgtggtgcc
     4621 gccggcattc ttcaccgcga cgggttgcgg caccgcatca tgggtgtggc cgcccaggat
     4681 cacgtcgatg ccattgatgc ggcttgcgag cttgaggtcg acatccatgc cgttatgcga
     4741 caacaacagg acgcaatcga ccttgtcctt gtcgcgcagc gtggcaacga gtttctgcat
     4801 ttcctcgtcg cggattccga acgtccagtt cggcgtgaag cgcttgggat gcgcgatcgg
     4861 cacatagggg aaggcctggc cgatcacggc aacgcggtgg ccgccgatct ccttgatggt
     4921 cgccggcttg aagacgcgac cagaggccgg atcgaacgct ttcgcgtcgt tgaaggcagc
     4981 ttcctcggtc agaaatacgt tctgcgccag gaactcgcct ttgaagcgct cgaggttggc
     5041 gcgcagcgcc gcttcgccat aggtaaattc ccaatgaccg gtcatcgcct cgatgccgag
     5101 caggttggcg gcctccacca tgtcggcgcc cagcatactg ttggccagac ccgtgccctg
     5161 ccacaaatcg ccgccgtcga ggagtagcga gtggccctcg ccgacatcgt tgcgcaggcg
     5221 gtcgatcagc gttttcagat gcgcgaagcc gccgagcttg ccgaaacgct gcgccgattt
     5281 ctcaaaatcg agcgaggtga aggcgtaggc ctccgcgctg tcggcgctga tcccgaagcg
     5341 gtcgagaaag gcttttccaa cgagatgcgg cggccggccc agcatctcgc cgatcccgat
     5401 attgacactc gcctcgcgga aataaaccgg cctgagttgc gcgtgaacgt cggtcagatg
     5461 caggatgcgc gcatttccga agcgttcgag gtcgtagagg cttggcgttt cagcggcgcg
     5521 cgcgtggcgg gcgccgccga gcaggagcgc tgcgccagtt gtcttcagaa aatcccgccg
     5581 gcggattttc attgcgatgc cgcctcacac atcttagcga atgaccgcat ccttccagcg
     5641 ctctttctcg ctggtcgcct ggaagatcga ggccttggcc agcgcctcgg cctcctttgc
     5701 cgccgcaagc gcggcatcga aatcgccggc ggaagcggat ttcttcgccg cagcaagcgt
     5761 tgcttccgtc gtggtccatt gattgtgcaa ggcacttgcc tctttctcgg cggcctcggc
     5821 tgcggcatag gcggccttga aatcggcttc ggacgcggca cgcgccggca tcgtgcccgc
     5881 gatcagcaac accgccgcaa gcgtcatatt ccacccgttc gacaggatcc tcatggccgc
     5941 gctcccggcc ccgagatcgg caagccgttg ctcatgtagg acaggaaata ttcgagatcg
     6001 cgatactcgt cggattgcgg gtcgagcggg accgcgcggg tctggctgtt gcaggtgacg
     6061 agccgccggc tggtcgtacc catgccgctc cattcggagc ggtagatcgg tagcgcgttc
     6121 atgataccga gcgcaggggc aagagtctcg gcacgaatgc gctcgcccgg gctctgcaca
     6181 tggcagctcg cgcaggagaa gttgagctgg ccgcgccgcg tatagaaata acgcttgccg
     6241 tcctcgtaag ccgcgagcgc gcgcggatcg tccgggacct tgatatccat cggcttgcgg
     6301 cgcgaggtga aggccatgta ggcggtgagc gaagccatct cgtccttcac ataggaatac
     6361 ggcgcttcgc cgttggcctc gcggcagcga ttgagggcga gctccagcgt gacgaccttg
     6421 ccttccttct cgtcgaaata gggatagttc tggcgaatgc cgatgccgcc gttcggaaag
     6481 caatccgcgt aggtcttgcc gttcctgaac ggcgtcgcaa acatttcctt gccggtttcg
     6541 agcgcaaact cataaggcgg gaactgctcc ttctcatccc actgccggcg catgtccgca
     6601 ttcatcgagt agggaccgtt ggcgaaatcc tcgagcttca cctgggggaa tttccgcacg
     6661 aagtaatcgc ggaacgcctt ggcgtcgccg gcaggatcgg gcgtgtcggc ggcggccgcc
     6721 gcgtgaataa aaatcgccaa cgcgagtgtg gccaacgcag actgcaaaag caaaggcacg
     6781 cgcatcgtca ctggatcttc gccgtcatcg tgtcggttgc gccggtgttg tcgacccagg
     6841 cgatcttgag gtcgtcgccc ttggccgctc ccttgaagct gaacttcaca taggggtctc
     6901 tggcgacagc cggaccccaa tcggccaaaa acacggtctt gccgttatgc tcgaaggtga
     6961 gctgctgaat gaaatgcgcc gggatcaggt cgcccttcgc atcctttacg aggccggtat
     7021 ccatcgggtg ctggatcagg gcctgcacct cggtgacgtc gccatttgaa atcgcgcgca
     7081 cgcggattgt cgatgccatg tattgctccc tgacctacga tttgcgcatg gtcttgtcgg
     7141 aaaaccgcct tccgctcttt cctatcatgc gcgtcagccg ccgcagccgc cgacggtgac
     7201 cttgacttcc ttggtcgcac taaacagctt gccctcggat tcgacgatcg cgatcacgtt
     7261 gcaggtcttc gccatcttca gccggttagc gacattcggc agcgtacctt ccggaattgc
     7321 ataggacgca gccaatgcgt tcgggttttc gctgacaaag aacgagatcg aggtgacacc
     7381 ggcaagcgtc gtcgttaccg acaccggcac caccgaaccg ttttcggcaa tctccggcgc
     7441 atcgagcttc accttgtcgg aggactccgc cgtccgtccg tagagcgcct tgatcgcatc
     7501 cgcgtcgctc ttctgcttga atgcgttctc cggatatttg tcattggcgg cggcgattgc
     7561 cggcatgggc aaaagcggaa ggttgcccag cccgatcagc gcaacggcgc cggcgccttt
     7621 gagcaccagg cggcgcgaga ggggaaggct atcgactgtc gtcatcaaaa aatctccaaa
     7681 aaaacgtgcc gtc
//
LOCUS       DATLJT010000199         5857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3915200,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000199 DATLJT010000000
VERSION     DATLJT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915200"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VM910_04545"
     CDS             <1..191
                     /locus_tag="VM910_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61852.1"
                     /translation="RSDEIAFGGDFAMAIASHLDDFDADPETKRVLHAALEMTRAALG
                     LAGDFANGIIAKQIVELC"
     gene            1580..1846
                     /locus_tag="VM910_04550"
     CDS             1580..1846
                     /locus_tag="VM910_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61853.1"
                     /translation="MNAMWRFLRDKRNQQVLGWLGGGLVVAATGLWAAFVYFFTPGKS
                     PEPQEPTPPSVQANCGGIAIGRDMTGSTITGGTITNSDCAPKTK"
     gene            1853..3979
                     /locus_tag="VM910_04555"
     CDS             1853..3979
                     /locus_tag="VM910_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI61854.1"
                     /translation="MKPHPCFVAAALGMYFFTLNTTAHAQQIKAETGGVAIGRDVIQS
                     TVVIGIPQEKVDELVRDAKRSLEELTTQQRENIALLKEKLDLNERQVRASLAILGEND
                     IPPERLAAKLVEIAERFKVLQETASAQSGDDPKIVALKTDAQGAIEVGELAKADAIFA
                     DVETEQRRALDRLALNAAETSARRGEISLARLRYAEAAKHFAKAAGVFPSNSAYEDKR
                     ISYLQQEATALFQQGAEFGDNGALLASIERYKRLVALAPRERVPLEWARIQDGLGIAL
                     TILGRRESGTAILGEAVATFREALKERTRERVPLDWATTQTNLGAALFRLGERESATA
                     KLEEAVAAYRQALKERTPEHAPLEWAMTQRNLGNALFRLGERESGTAKFEEAIAAYRA
                     ALKEWTRELAPLQWALTNNDLGVALFSLGWRENDRAKFEDAVMVYREALKEQTRERVP
                     LAWAQTQNNLGLALWHLGEREGRTARLEEAVVAYREALKERTRERAPLDWAQTQSNLG
                     NALSSLGEHENGTVKLEEAVAAYRDALKEWTRERVPLDWAQTQSNLGAALWRLGERES
                     GTAKLEEAIAAYREALKERTRGRTPREWAVTQNNLGIALFRLGEREGGTRKLEEAIAA
                     YREALKEHTPERYPLDWAGTLGNEGLALMLLSERRRDAPMAVVALSQITKAVETMRSG
                     GDAPSAAFYEQQVPRARAIVERLRGQ"
     gene            4296..4469
                     /locus_tag="VM910_04560"
                     /pseudo
     CDS             4296..4469
                     /locus_tag="VM910_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
     gene            complement(4520..4648)
                     /locus_tag="VM910_04565"
     CDS             complement(4520..4648)
                     /locus_tag="VM910_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61855.1"
                     /translation="MQFDQLRRRDLITLLGGAAAAWPLAAARSRRDADNRGRPLLG"
     gene            4995..5306
                     /locus_tag="VM910_04570"
     CDS             4995..5306
                     /locus_tag="VM910_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI61856.1"
                     /translation="MQMQHIRYFLALCEERSFTRAAKRCGVSQPSLTNAIMALEQELG
                     GVLFQRKPLVALTVLGHAVHPYFDRIAENAAHAREAAQAMADVRRSVSMPLPPSGLSC
                     T"
BASE COUNT         1214 a         1735 c         1794 g         1114 t
ORIGIN      
        1 tacgcagtga cgaaattgcc tttggcgggg attttgccat ggctatcgcg tcccacttag
       61 acgacttcga cgccgatcca gaaaccaagc gagttctcca tgccgcgctc gaaatgacgc
      121 gcgcagccct tgggcttgca ggcgattttg cgaacggaat catcgccaag cagatcgtcg
      181 aactttgcta gggccggcta gcggcatccc gacgttttat gcgaaggcgc attgaagaaa
      241 ttacgcgggc atttgttcgg ggactaacgg gagcaccgtc caagcgcccc ccttcgccgc
      301 tccccgagca attagccagc cttcttgtgt tcggacggcc gaccagcttt ctgcggcgtc
      361 cgatgatgcg ttgcatggcg gtcggtcccg tcctccttcc ggctacgcgc acgcggctgg
      421 taccgtttgt ccctcgcgcc gcgcgctacg gtttcggcga ggtgcgtgtc gctaggctag
      481 cattgctgat ctttactgcg atgtcaggat cacattcctg cggggttgtt atcaccaaat
      541 tctcggacca cgtcgtgacc ttccgaagct ccccaacggt ctttgcggac acgccggtgc
      601 ctacgtccgc ctcaacgtgc atcttggctg gcaagctggg gagaactatg tcgagcgcag
      661 caatgctttt gcacttgtac cggggcatag gctcctccct gattgctcgg ctgctgaaca
      721 gccaaccatt gaaggcactt ctttcgcgcg acatgcgtcg gagcgagggc acgggacgct
      781 cgaaggggtc tcagacaaca agttcgatga agtgccgaac gctttggggt ggcgtctctg
      841 tcgctctaat tctgtgattt tacccaaagc agggaagccc gaaaacccag aaaaatgcga
      901 gttcatcgca actcacggaa gcccgggctg acagcgagag cgccaatgca aaagaggtaa
      961 atcgctagcg gcggccgccg cgagaacccg ctggtggggc gggactccct ccacgcatcc
     1021 cggggggacg cacgattgca ggcccaacag atgatccact ttgcgaagac ccgcttgatg
     1081 agacacctac tgaacgcccc gttgattgca gttggaacgt cgatggcggt tgcggttgga
     1141 acgttccgct tgaggaacaa cctctcacgc aggcagcacg agcgcgttgg tcagcaattt
     1201 tacgacagcc gcatgcgtcc tgtgcatgca cctcaatgat cgacattagc aagaagaact
     1261 gcgcagttgc gacgacaaca gtcgaacggg acacgcttag cacccctttc gatagctaca
     1321 gaaagattac tgcccgagat aatttgaaac aatcaaagat ggtttcgaac gtcaggtttt
     1381 tgcaatgagg gttaacgcaa caagctacta cgcccgagca agccgtattg cgagccatcc
     1441 cggctagata cagccaaggc tgcgcggcca gatcacacgg acaaaatggg gctcagcgga
     1501 gaggtccacc cgcccgggct cacggatgcg ccgcgtatta gcctatgtta ttatagacgt
     1561 tacatgttgg acaactgtca tgaacgccat gtggcggttc ttgcgtgaca agcgcaatca
     1621 gcaggtcctg ggctggctcg gaggcgggct ggtcgttgcg gcaacgggac tttgggccgc
     1681 ttttgtctat ttctttactc ctgggaaatc tccagaaccg caagagccga cgcctcccag
     1741 cgtccaagca aactgtggcg gcattgccat tggccgcgat atgactggct cgaccatcac
     1801 gggaggtact atcaccaatt cggattgcgc gccgaaaacg aagtgaggcg ctatgaagcc
     1861 gcatccgtgc ttcgttgcag cagcgttggg catgtatttc ttcactttaa atacgaccgc
     1921 ccatgcccaa caaatcaaag ccgagacagg tggggtcgca atcggtcgcg acgtcattca
     1981 atcaactgtc gtcatcggca tcccacaaga gaaagtggac gagcttgttc gcgatgcgaa
     2041 gcgctctctt gaggagctga ctactcaaca acgggagaac atcgctctac tcaaggaaaa
     2101 actagacctc aacgagcgtc aggtgcgcgc ctcgctggct attcttggcg aaaacgacat
     2161 tcccccggag cgattggcgg ccaagctcgt tgaaatcgct gagcgcttta aagtccttca
     2221 ggaaacggca tcagctcagt ccggcgacga ccccaaaatc gttgccctga aaactgacgc
     2281 gcagggggcc atcgaagtag gcgaactggc caaggcggat gccattttcg cggatgtaga
     2341 gacggagcag aggcgtgctc tcgaccgcct cgctctcaac gcggccgaaa cgtcagcccg
     2401 ccgcggtgag atttcgctcg ctcgattgcg ttatgccgaa gccgccaagc attttgccaa
     2461 ggccgctggc gtgtttcctt cgaacagcgc ttacgaggac aagcggatta gctacctcca
     2521 acaagaggcg accgccctct tccagcaggg agccgaattt ggtgacaacg gcgccttgct
     2581 cgcgtccatt gagcgataca agcggttagt cgctttggca cctcgcgagc gcgtgccgct
     2641 cgagtgggcc cgcattcagg acggactggg cattgcgctt actattttag gcaggcgcga
     2701 gagcgggacg gcaatcctcg gagaggccgt tgccaccttc cgcgaggcgt tgaaggaaag
     2761 gacccgcgag cgggtgccgc tcgattgggc gacgacccag actaatctgg gcgctgccct
     2821 attccggctc ggcgagcgcg aaagcgcgac ggcgaagctc gaagaggcag tcgccgccta
     2881 tcgacaggca ctgaaggagc ggacccctga gcacgcgccg ctggaatggg ccatgaccca
     2941 acgtaatcta ggcaacgcgc tattccgcct tggagagcgt gagagtggga cggcgaagtt
     3001 cgaagaggca attgctgcct atcgggctgc cctgaaggaa tggacacgtg aacttgcccc
     3061 gctccaatgg gctctgacga ataacgatct tggcgttgcg ctattcagtc tcgggtggcg
     3121 tgagaacgat agagcgaagt tcgaagacgc ggttatggtc tatcgcgagg cgctgaagga
     3181 gcaaacccgg gagcgagtac cgctcgcctg ggctcagacg cagaacaatc tggggctcgc
     3241 cctctggcac cttggggagc gcgagggtag aacggcaagg ctggaagagg cagtcgttgc
     3301 ttatcgcgag gcgctaaagg agcggacccg cgagcgcgcg ccactcgact gggcccagac
     3361 ccagagcaat ctgggcaatg ctctttctag tcttggagag cacgagaatg ggacggtgaa
     3421 gctcgaagag gcagttgctg cctatcgcga tgcattgaag gaatggaccc gcgagcgagt
     3481 gccactcgac tgggcacaga cccagagcaa tttgggagct gccctctggc ggctcgggga
     3541 gcgcgagagc ggcacggcaa agctggaaga ggcgatcgcc gcctatcgtg aggcgttaaa
     3601 ggaaagaacg cgcgggcgca ctccgcgcga gtgggccgtg acccagaaca atctgggcat
     3661 tgcactcttc aggctcggag agcgcgaggg cggaaccagg aaactggaag aggccatcgc
     3721 tgcttatcgc gaagcactga aggaacacac ccccgagcgg tacccgctcg actgggcggg
     3781 gactcttggc aacgagggcc tcgccttgat gctgctctcc gagcggcggc gggatgcccc
     3841 tatggcggtg gttgcactca gccagatcac taaggcggtc gaaacaatgc gaagcggcgg
     3901 cgatgcgcca agtgcggcgt tttacgaaca acaagtcccc agggcacgcg ccattgtcga
     3961 gcgtctgcgc ggacagtgag aacccaccat gagcggcatc tgtccttcgt ctcagcactg
     4021 ggcatgtgct tagcttggtc gtcgtttgtc gaacgatgac cacggccgcc tctaccgaca
     4081 tagggggcag tttgcgggca atagccgatt tggcccgtga ccgggcggcg gcttgatcgt
     4141 gccgcctgac aatttttcct atgttcaccg cgcgctgatt ttcgcgttgg cggctcgcca
     4201 ccgcgtcccc gcggtctacc cactccgctt catggcgcgc gaagggggct tggtatccta
     4261 cggggtggaa ctcggcgaga cgtttccgcg cgcggccgag tacatcgatc gcatcctgca
     4321 gggcgaaggc catggagaag cggctcgacg ccacgctcgc cgcggtgcag accgtgcagc
     4381 ctgcgcttac gaaattctac aattccctga gcgacgaaca gaaggcccac ctcaattcgc
     4441 tccgttcggt gtcccggccg acggggtgac ggaggacgcc agtcagcggg ctcaagccta
     4501 gtggtcgggc tagccgccat taaccgagta acggccgccc tcgattgtcg gcatcgcgcc
     4561 tgctgcgcgc cgccgcgagc ggccacgcgg ccgccgcgcc gccgagcagc gtgatgaggt
     4621 cgcgccgtct caattggtcg aattgcattc ggtccccgcc agccagggct ggattggagg
     4681 atatcgaatt ggcagcgatc agtcaggagg taacggaacg attctacaac ctgtaagctg
     4741 tcggcgaggc cggcgcatgt cccagatggg tgagcacttc cattcgtgcc aatagctgcc
     4801 atgccgcaaa tggaagtgaa gtgcttcttc agcggccctt ttattagccc ttgtagccgc
     4861 tcgcgccctt gcgaggccgg agtcggcagc gttaccggca aggcagtcca ctctgtgtga
     4921 actacctcac acttagaaac cggtgccgat ggcagtttag gagtcgaggc gcgattgata
     4981 ccgcgaggtg aaacatgcaa atgcagcaca tccgctactt tctggcgctt tgcgaggagc
     5041 gtagcttcac ccgcgcggcc aaacgctgcg gcgtttccca accctcgctg accaatgcca
     5101 tcatggcgtt ggagcaggaa cttggcggcg tgctttttca acgcaagccc ctcgtcgcgc
     5161 tcaccgtgct cggccacgcc gtccaccctt atttcgaccg gatcgccgag aatgccgccc
     5221 atgcgcgcga ggccgcgcag gcgatggccg acgtgcggag gtcagtgtct atgccattac
     5281 cgccgtctgg cctcagttgc acatgacccg ccgtggacgt tgacggcggg atccgtcatg
     5341 taatcggagc tgcggctagc gagatcggcg accgcatcga ctgcatccaa cggtaactcc
     5401 agtcaagtcg ctcgactcga accgatggcg ggtttcggca gcagcagcgg tacaggcaag
     5461 agcttgaacg ctgcacctct cagtgctgac acatcacagc cgtcgttgct gacgacaccg
     5521 gagcacgtat gagggcggat cgacgttagg cagcggctat ggcgatccta aaggttcgcc
     5581 atcttctgag attaacttca acagatgtaa cctcgatcac gcatggccgg cccaccgtcc
     5641 tcgataatat cactcatcga ggccggcgcg gaccgaaagc cgtgcacgtc gaagtctggc
     5701 acggttcacg tatccaagcg tcaggcagca gctttgttct tgattgcgcc aatgatcgcc
     5761 gtgagtatgg cgccgccagc gcctccggcg atcacctgtg agatgatccc ttcgacgctc
     5821 agattgcccg actgcgctgc ggccgtgatc gccggca
//
LOCUS       DATLJT010000200         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_469091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000200 DATLJT010000000
VERSION     DATLJT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VM910_04575"
     CDS             complement(<1..342)
                     /locus_tag="VM910_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HVI61857.1"
                     /translation="MITPAEARSGNGHRDENFPVASRLIHPRHRAPILAFYEFVRTAD
                     DIADHSALAPPEKLALMDRLEATLLGKSNDDPVGVALRSQLAARQLSPRHAQDLLAAF
                     RMDVTKLRYRDW"
     gene            complement(339..1484)
                     /locus_tag="VM910_04580"
     CDS             complement(339..1484)
                     /locus_tag="VM910_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI61858.1"
                     /translation="MLAVASIVLAIWLYLLLARGAFWRCSERDDWGPAELPTWPRVAA
                     VVPARDEADCIGDSIGSLLSQDYGGAWTVILVDDDSSDGTAEIARRVANADARLRVVG
                     SRGLPAGWTGKLWVAKQGVDAASALPQPPDYLLLTDADIVHAPDSVSRLVAHAERRHL
                     VLTSLMVKLNCESFAERATIPAFIFFFQMLYPFPCVNRPQSAVAAAAGGCMLVRADAL
                     RKAGGIEAVRAALIDDCALAKALKAQGPIWLGLTERVRSVRRYPALADIRRMVARSAY
                     AQLRYSPLVLLGTIAGMALTYLVPALLAIFGSDAARWIGAGTCALMFIAFQPTLRFYR
                     LSPLWGFALPFIALQYMLFTLDSAYQYVRGEGGSWKGRVQANVSERR"
     gene            1757..>2647
                     /locus_tag="VM910_04585"
     CDS             1757..>2647
                     /locus_tag="VM910_04585"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HVI61859.1"
                     /translation="MARVDPVKKTAVDADATFQDRLARVARDTQALLDRLLASAPIDG
                     ERTRPARLLDVMRYVSLGGGKRLRPFLVVESAALFDVSRQNSLMAGAALECVHCYSLA
                     HDDLPAMDNDDLRRGRPTAHKAFDEASAILAGDGLLTFAFDILSRPETHPDVEVRLEL
                     VAALARAAGLGGMVGGQMLDLAAEGRFDASAPKLGEQDVKVLQAMKTGALLRFGCVAG
                     SILDKANQRQRTALKRYGSALGEAFQIADDLLDVEGDPATVGKATGKDAAAHKATLVG
                     VLGAAAARRRLENLVGEAQAA"
BASE COUNT          497 a          848 c          875 g          427 t
ORIGIN      
        1 ccagtcgcgg taacgcagct tggtcacatc cattcgaaac gcggcgagca aatcttgcgc
       61 gtgacgcggc gagagctggc gtgccgcgag ctgcgagcgc aaagcgacgc cgaccggatc
      121 gtcgttgctt ttgccaagca aagtcgcttc gagccgatcc atcagcgcaa gtttttccgg
      181 cggcgcaagc gcggaatggt cggcgatgtc gtcggcggta cgcacgaatt cgtagaatgc
      241 gaggatcggc gcccgatggc gcgggtgaat tagccgtgat gccaccggaa aattctcgtc
      301 ccggtggcca ttgcccgatc gcgcttcggc gggggtgatc atcgccgctc cgacacgttg
      361 gcctgcaccc tgcccttcca gcttccgccc tctcctcgca catattgata ggccgaatcg
      421 agggtgaaga gcatgtactg caaggcaata aaaggcagcg cgaaacccca taagggcgac
      481 aaccggtaga atcgcaacgt cggctggaag gcgatgaaca tcagcgcgca ggttccagcc
      541 ccgatccacc gcgccgcgtc gctgccgaaa atagccagca gcgcaggcac gagataggtc
      601 agcgccatcc ctgcgatcgt tcccaacagc acaagcggag agtagcgcag ctgcgcatag
      661 gccgaacgcg ccaccatgcg gcggatatcg gcgagcgccg ggtaacgacg gacgctgcgc
      721 acgcgttcgg ttaatccgag ccagatcggt ccctgagctt tgagggcttt cgccaaggcg
      781 cagtcatcga tgagagctgc gcgtacggcc tcgatcccgc cggccttgcg aagggcgtca
      841 gcgcgaacca gcatgcagcc gccggcggcc gccgcgactg cgctctgcgg tcgattcacg
      901 caggggaacg gatagagcat ctggaagaaa aagatgaatg ccggaatagt tgcgcgctcg
      961 gcgaagcttt cgcagttgag cttgaccata agggacgtca gtacgaggtg ccgccgctcg
     1021 gcgtgcgcga caagacggct gacggaatcg ggcgcgtgca cgatatcggc gtctgtgagc
     1081 agcagataat ccggcggctg cggcagcgcc gaggcggcat cgaccccctg ctttgccacc
     1141 cagagcttgc ctgtccaccc cgccggcaag ccgcggctcc ccaccacgcg cagtcgggcg
     1201 tcggcgttcg caacccggcg cgcaatttcg gcggtgccat cgctgctatc gtcgtcgacc
     1261 aggatgaccg tccacgcacc gccatagtcc tgcgagagga gcgatccgat gctgtcgccg
     1321 atgcagtccg cctcgtcgcg agccggcacc acggctgcga cccgcggcca tgtcgggagt
     1381 tctgccggcc cccaatcgtc gcgttcgctg cagcgccaaa aggctccgcg agcgaggagc
     1441 agatagagcc agatcgcaag cacaatcgaa gcaacagcta gcacgcgagc aaagcctccg
     1501 cggtatctct ggcatagcgg ccgatcccgg gccccgccga ccgggtcagc aaaaacaatt
     1561 caacatattc aatgaattgg gttaggaaaa gtttggacgg gcgggtcgcc cttgctaaat
     1621 tgccgggcgg cccgcctgcg ggctcttgtg gatgccggtc ggccgcccgg gatgggggcc
     1681 aacttaacga gttgcgcgcg ttcggacgca aggttggaat ttacctcctg ctgaacgcgc
     1741 tctgggggcg gaacgcatgg cccgcgtcga tcctgtgaaa aaaacggccg ttgacgccga
     1801 cgcgaccttt caagaccggc tcgctcgagt tgcccgcgac acgcaagcgc tgctcgatcg
     1861 cctgctcgct tcggcgccga tcgatggcga acggacgcgg ccggcgcggc tgctcgatgt
     1921 gatgcgctat gtgagcctcg gcggcggcaa gcgcttgcga ccgttcctgg tggtggaatc
     1981 ggcggcgctg ttcgatgtct cgcgccaaaa ttcgctgatg gcgggcgcag cgctggaatg
     2041 cgtgcattgc tattcgctgg ctcacgatga tctccccgcc atggacaatg acgacctgcg
     2101 acggggccgg ccaaccgcgc acaaggcatt cgacgaggcc agcgccattc tggcgggcga
     2161 cggccttctc accttcgcct tcgacattct ttcgcgtccc gagacgcatc ctgacgtcga
     2221 ggtcaggctc gaactcgtcg cggcgctcgc gcgcgctgcc ggcctaggcg ggatggtggg
     2281 cgggcaaatg ctcgacctcg cggccgaagg acggttcgat gcttctgcgc ccaagctcgg
     2341 cgagcaagat gtcaaggtct tgcaggcgat gaaaaccgga gcgctcctgc gcttcggatg
     2401 cgttgccggg tcgatcctgg acaaggccaa ccaaaggcag cggaccgcgc tcaagcgtta
     2461 cggttccgcg cttggcgaag cgttccagat cgccgacgat ctcctcgacg tcgaaggcga
     2521 tccggcaacg gtcggcaaag cgacgggcaa ggacgcggcc gcccataagg caacgctggt
     2581 cggcgtgctc ggagccgcgg ccgcgcggcg gcggctcgaa aacctggtcg gcgaggcgca
     2641 ggccgcg
//
LOCUS       DATLJT010000201         1567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2307158,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000201 DATLJT010000000
VERSION     DATLJT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307158"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            795..1446
                     /locus_tag="VM910_04590"
                     /pseudo
     CDS             795..1446
                     /locus_tag="VM910_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511202.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          296 a          527 c          430 g          314 t
ORIGIN      
        1 caatgacggc gtcctgcctc cacggcttca gaacgatgag ggaaatcagc aagctgatga
       61 tgatgacaac cctgttgatc gtaagcagcg atgaaaaccg tcctgctacg cgtccccgga
      121 tcgagctcag cgctccgctg gctggtgttt gcttgcccgc cccgcgctct cgacccgagg
      181 gcgctccatc ttgtcgtctt ccgagagtct cctcgggcag cacttctggg tcgcggtagg
      241 gcaacactgc tgcaggatcg tttccagaca tcgtacacct cctgcgtgct gggcgacctg
      301 ccgccgcctc attgttaccc tattaatgcc acttttctgt gcgcgcggat gtgatgtcca
      361 tcacacaaac gttgattggt agcgagatac caccgctacg ggcttcgagc cgctttttgc
      421 tacggccggg ctggccgttc gcgaaaacaa cggcgacctg tgtacggttc gtcacgccga
      481 acttgcgcat gatctgccgg acatgaactt tgaccgtact tccctcgtcc ccagccggcg
      541 cgcaataacc ttgttcgtgt cgcctcggct caggagttcg aggaccgcct tctgcctcgc
      601 tgttagactg cgacttttca ctgcgtcttc cgaatttttt gcagcgccat tgtccccgtt
      661 catgacacaa gacggggttg cgatgcaatc gatcgccgga tgcctctgcg ccagataagt
      721 gtgttttagc tgcatggccg acggaaaata agatccgccg ttgagcacga acgacaaggc
      781 ccgtagcgct agctcagcat tcgctcccgc gttggcgcca gcggatgggt gaagacaagc
      841 tcaaggcgct gttgcaagag agcctggcgg tggcgacccg gacggaggcg atgaagccgt
      901 ccgacctcgc ctgctcaacc gggcgcgcga gcggctggtg cgactgacga agaagctcgg
      961 agtgagcctg cgccaatcct acgcgcgggt cggcaagctg gctctgatca agcatcagcg
     1021 ctacgcccat gccccgccac cgtggtgaaa cattccgccg gcgggcagtt cgtcacccac
     1081 gccgcagcac tgccccgcaa tccctatgac gggcacacgc tcgccacggt cattccgcag
     1141 atgcaagcgc tcatcggcaa catcctcgat cgctgcatca ccgatgccgg ctatcgcggc
     1201 cacaacgccc ctcccgatca caagttcaag gtctacacga gcggccagaa gctccgcctc
     1261 acgccgcaga tctagcgcga gttcaaacgg cgcgccgcca tcgagcccgt catcggccac
     1321 ctcaaggaac accaccgcat aggccgaaat caccttgccc atgccagcgg cgacgccatc
     1381 aacgccgtgc tcgccgccgt cggctacaac tttcgccgcc tcctcgcatg gttgaggctt
     1441 ttgttgctca gaaatcctga ttgcgctcag cctacctgcc cagcgcaaat ccgtctgaaa
     1501 tcgggttctt caccgccgac ctgtgtaagt tgcaagcccg acatcgtcac cgctggatcc
     1561 tcttggt
//
LOCUS       DATLJT010000202         5462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_928614,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000202 DATLJT010000000
VERSION     DATLJT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928614"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(878..1507)
                     /locus_tag="VM910_04595"
     CDS             complement(878..1507)
                     /locus_tag="VM910_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVI61860.1"
                     /translation="MKGWPPSGDVHEYDPASDRWQRLAALPTTRGALAAAVLDGKIHA
                     AGGIGWRGRNTAAHEVYDPAANRWTALAYIPTARDHLAAAAIDGRLYAVGGRIDGNCS
                     RNLSSNEAYDLATDRWEQRAPMPTARSGIATAVLEGRMFVFGGEAPAGTFNQVQAYDG
                     RSNGWSNHARMPTARHGLGAAAVAGRIYVVSGGPTPGDSVSAANKIFVP"
     gene            complement(1479..1595)
                     /locus_tag="VM910_04600"
                     /pseudo
     CDS             complement(1479..1595)
                     /locus_tag="VM910_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
     gene            2309..3340
                     /locus_tag="VM910_04605"
     CDS             2309..3340
                     /locus_tag="VM910_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI61861.1"
                     /translation="MDYFAGLDVSVKETSVCIVDDAGKIVREVRVASEPEALLQVLTN
                     KTYRFKRIGLEAGPLSQWLYSVLAEAGLPVICVETRHMRAVLKAQINKTDRNDARGIA
                     QMMRVGLYRPVHVKTLRSQKLRMLLTHRKLLQSKAIAIENDLRGTLRNFGLKVGMVGT
                     VKFEARFKELVANLPDLAVLVEPLLIVRRVVREQLGILHRRVLAIVRDDDVCRRLMTI
                     PGVGAVVALTYRATVDVPARFRNSKAVGAVFGLTPAKYQSGENDRTGAISRCGDEMMR
                     MMLYEAAQIMLVRSAKWSWLKAWAMKIARHRGMKKAIVALARRLAVIMHRIWVDGTEF
                     RWTREVAAA"
     gene            complement(4210..>5462)
                     /locus_tag="VM910_04610"
     CDS             complement(4210..>5462)
                     /locus_tag="VM910_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVI61862.1"
                     /translation="YRGGDGPLTTRFNRYEDPLGEAFLEAGASAGHPFTVDYNAEQQE
                     GFGRLQSTVRHGHRCSTAVAYLRPALERPNLTLKVTALATRIVFQGTRATGMEYVQRG
                     KTHSAHAAREVILAGGVINTPQLLMLSGIGDPAELKAVGIDARVALSGVGKNLQDHIS
                     APIAYARNEPGPLHRAMRLDRIARELATAYFVGKGIATDLPAGAMAFLRSPYAESLPD
                     VQFIFISAPMTAGPYLAPFKQGYQDGFAIRAVLLRPESRGFVRLKSADPCGSPAIVQN
                     FLSTQRDWEVLRAGLRMAEEVGAQAPLRPFAGKRLAPLSDADLNKHVAETGITVHHPL
                     GTCKMGIASDPTAVVDTKLNVFGTEALRVVDASVMPDLVGGNINAPVIMIAERASDII
                     RGRAVLPPATISEAAAKVTVMEAG"
BASE COUNT         1059 a         1684 c         1701 g         1018 t
ORIGIN      
        1 cagcgtcaag ccttccggca cctcgaaggt gcgcggcagc gcgtcgccca ccgcctttcg
       61 gatcaagccg tcgccgggcg cggagacccg cgcgtgctgc gccctgagat cgcgccgctc
      121 gcgctcgtct tcccgcatct ccgcgatcag ctcagcctcg ctcgacatct tcaataatcc
      181 tctacagacg gcacgccgat cccaacgcag atggcgcggc gctccgcggc gcagattgcc
      241 agcatatctt tgttagtcgg cgatcggacc agcgccagct ggtcagctac ggcgcgcaag
      301 aacttctcgc gccagcgcgg cggaacaacg ctggctctag tttggacgat ggcgagctgc
      361 tcaggtgtga gagcgctcac aacagcagtg gccgcgccgc ttgcgcaatg cgccgccggt
      421 agaaaaccga cggtggcatc gacattgcga gccgttcggg caaggacagc gtcatcaaca
      481 gcttctgggg aaggcggctg cgacctcacc acgccgccgc gccgccgagc agggtgatga
      541 actcgcggcg tctcaggtcg cgcatgggct cctccccggc gtccagttgg cgtccctacc
      601 caattgccag gcggttatcc cgtcgcttta ggccggaact tatacccaca gaagcatgac
      661 caggatgacc aggatcgaat acaccacgac ccacacgatg tcggaagccc aaccccgtct
      721 ctcctctaca gcctgctcgc tcatggcgaa ttctcgttgg ggtcgcaatg caaggtccct
      781 tccccccact cagcctgcca ccgaacagat ggacaggtcc ttgggacaaa cctgaattgt
      841 tctgaatcga gggccgctcc acggtgcgag acctgcctca cggcacgaaa atcttattgg
      901 cggccgaaac cgagtcaccg ggcgtcggcc cgcccgagac gacgtaaatc ctgcccgcga
      961 ccgccgctgc gccgaggccg tgtcgggccg tcggcatacg cgcatgattg ctccagccat
     1021 tgctcctgcc atcgtaggct tgcacttggt tgaaggtgcc cgccggcgcc tcgcccccga
     1081 acacgaacat ccgcccctca agcactgccg tcgcgatgcc gctgcgcgcc gtgggcatcg
     1141 gcgctcgttg ctcccagcga tcggttgcga ggtcatacgc ctcgttcgac gacaaattgc
     1201 gcgaacagtt gccgtcgatg cgtccaccca ccgcgtagag gcgtccgtct atcgcagcgg
     1261 ccgccaagtg atcgcgcgcc gtcgggatat aggcaagcgc cgtccaccgg ttggcagcag
     1321 gatcgtaaac ctcgtgcgcc gcggtgttgc ggccgcgcca gccaatgccg ccggccgcgt
     1381 ggatcttgcc atcgagtacg gcggccgcca gcgcgccgcg cgttgtgggg agggctgcaa
     1441 gtcgttgcca acgatcgctc gccgggtcgt attcgtgtac gtcgccgctc ggcggccagc
     1501 ccttcacggg cgcgggtcaa ctccttctcc ttcacgagca gttgctggcg cgcagcctcc
     1561 cactcctgcc gcgagacgat cgggggtgtc ttcattgaaa tgtcctcctt atcagttcag
     1621 gtccaacgct cgtacgctcg ccctggtttc cggccctttg cttgtcactt gacacgctct
     1681 gcttctgcga gtgccgcata ctgtcgtact cgtcgtggcg gcgggggcgt cgcggcggaa
     1741 gttgcattcg gggaacacga tagaacggga cgtctgctcg ggggtcagac tcggtcattc
     1801 ggcgatgtcg gctcaatgtc cgcatcaccc cctgtcaccg catcgggtga ccagtcggtt
     1861 agcgaactac cgcccggctg tccgcgcgcc gatgggacca tcagcctcag cggcggctgg
     1921 cgcgacagtt ttctcgcagg ggaaaactca atccaccctc cttagtccta ttcctcgggt
     1981 cgcttttcag gaggcacagc ggattcgacc ccgattggac gagtgcccgc attccacaat
     2041 gcgatgatca accatgcgac tgaaaatccg agaaaaccgc cagcaacaag gtcgctcacg
     2101 aagtgatagt tggcgcctag ctctccaata aagaccgttg caatgcagat cgcgtagatg
     2161 gggcggaagc gtgcccacat gacccaaaag atggggtaat cggcctacca gcttgtggat
     2221 agctgaagaa aaagggtgct gcgcttcagg ctgatggtga aacgaggtcc gcgtatgcgg
     2281 cctcaagcat cagaaggaga agcgcagcgt ggactatttt gctggactcg acgtgtcggt
     2341 caaggaaacg agcgtctgca ttgtggatga tgcgggcaag attgtgcggg aagtgagggt
     2401 ggcgagtgag cccgaagctc tcctgcaggt gctgacgaac aaaacctatc gcttcaagcg
     2461 aattgggctg gaagccggac cgctgtcgca gtggctttac agcgttctcg ccgaagcggg
     2521 cttgccggtg atttgtgttg agacgcggca catgcgggcc gtgctgaagg cgcagatcaa
     2581 caagaccgat cgcaacgatg cccgcggcat cgcgcagatg atgcgggtgg ggctctatcg
     2641 accggtgcat gtgaagacgt tacgcagtca gaagcttcgg atgctgctga cccatcgcaa
     2701 gctgcttcag tcgaaggcca ttgctatcga gaacgacctg cgtggcacct tgcgcaactt
     2761 cggtctcaag gtcggcatgg tggggacggt caagttcgag gcgcggttca aggagcttgt
     2821 cgcaaacctt cccgacttgg ccgtcctggt ggaaccgctg ctcattgtcc ggcgggtggt
     2881 tcgcgagcag ctcggcatcc tgcatcgccg cgtgctggcc atcgtccgcg acgatgatgt
     2941 gtgccggcgc ctgatgacga ttcccggggt cggtgctgtg gtcgcgctga cctatcgcgc
     3001 aaccgttgat gtgccggccc ggttccgaaa ctccaaggcg gttggggcgg tgtttggact
     3061 gacacccgcc aagtaccaat ccggcgagaa cgatcgaacc ggcgcaatat cacgatgcgg
     3121 agacgagatg atgcgaatga tgctctatga agcggcccag atcatgctgg tgcgttcggc
     3181 gaaatggtcc tggctcaagg cctgggcgat gaagatcgcc aggcatcgtg ggatgaagaa
     3241 ggcgatcgtg gcgctggcgc ggcggctggc cgtgatcatg caccgcatct gggttgatgg
     3301 taccgaattc cggtggacca gggaggttgc cgcagcgtga caactgtaca ggatcaaatc
     3361 agatagaaga aaacctgaga tccatccaac ggtggaatga tgtccctcgc gggacgatgg
     3421 atgaagtgag ttcgtatgtc ggtttgaacc tgccgtcaca ctgacggcaa ggtcgcccat
     3481 tagattgttc cacctcgtcc tcgaatccca tgatggaagg gccttggtgc cgagtccgaa
     3541 gagaagcgcg agcccgtgag tgacatcaac actggagttg gtggatggcc tgaaagtgct
     3601 tgacctcaga cggccgatta gagaagaccg acataattgt gcagatggcg accatgtgac
     3661 cggatggaaa agcagcgaaa ccagggcctc cgtgaaatgg attgaagttg tagacgccat
     3721 cccggatgag cgagggactg tcttgcaccc acgtctcggg ccatgtgcga ccgaaggcaa
     3781 acttgagcca attctcgggc gcatcagaaa cagctaggct taatgccgaa agaacgatcg
     3841 tagtctgaat ttcggccaat gccgccttgt cacggcccgc acacctagca cgagtgtgca
     3901 agcagtgacg aacggaccga tcaatttggg cagtcgcgta gcaacgtcaa agatgtcgcg
     3961 atatccccga agctcgttgt gaacaaaata agcaaccggg cggtcaatcc aataatacga
     4021 tacagtgata cagagcacgc aaatcgtcag cgcgattgcc tattttcgca ggcccgttat
     4081 ccccacgcct ttggagcact tagttcgagg tcttggggcg atattcacga cagcgtcccc
     4141 gcgctcgaac ccgcgagccg ggtgagaatt cgtgagctac attgggcggt cctcgatcca
     4201 gccacacctc taccctgctt ccataacggt gaccttggct gcggcttccg agatggttgc
     4261 aggcggcagg acagcgcggc cacgaatgat gtcggacgct ctctcggcta tcatgatgac
     4321 gggcgcattg atgttgccgc cgacaaggtc cggcatcacc gaggcatcga ccacccgcaa
     4381 ggcttcggtg ccgaacacat tcagcttggt gtcgaccacc gccgtcgggt cgctcgcgat
     4441 gcccatcttg cacgtcccga gcgggtgatg aacggtgata ccggtctcgg cgacgtgctt
     4501 gttcagatcg gcatcgctca gcggcgccag acgctttcca gcgaacggcc gcagcggcgc
     4561 ctgggcgccg acctcctcag ccatccgcag accggcgcgc agaacctccc aatcgcgctg
     4621 ggtggagagg aagttctgca cgatcgccgg tgacccacaa ggatcggcgg atttcaaccg
     4681 cacgaaacca cggctctccg gccgtaacaa cacggcgcgg atggcgaagc cgtcctgata
     4741 gccttgcttg aacggcgcaa gatatggtcc cgccgtcatt ggcgcgctga tgaagatgaa
     4801 ctgtacgtcg ggcaggctct ccgcgtaggg actgcgcaga aaggccatcg caccggccgg
     4861 tagatccgta gcgatgccct tgcccacgaa gtacgcggtc gcgagctcgc gcgcgattcg
     4921 atcgaggcgc atagcacgat gcagtggccc cggctcgttg cgcgcgtagg caatgggcgc
     4981 cgagatgtgg tcctgtagat tcttgccgac gcctgacaac gcaacgcgcg cgtcgatgcc
     5041 gaccgctttc agttccgcgg gatccccgat gcccgacagc atcagcagct gaggtgtatt
     5101 gatcacgcct cccgcgagaa tgacctcgcg cgcggcgtga gcgctgtgcg tcttgccgcg
     5161 ttgcacatat tccatgccgg tcgcgcgcgt gccctgaaac acaatgcgtg tggccagcgc
     5221 cgtgaccttc agcgtgaggt tcggtcgctc gagcgcgggc cgaaggtagg cgaccgcggt
     5281 gctgcagcga tggccgtggc ggacagtgga ttgcaaccgg ccgaaaccct cctgctgctc
     5341 cgcgttgtag tccacggtga acggatgccc ggcgctcgcg ccggcttcca gaaacgcctc
     5401 gccaagcgga tcttcatagc ggttgaaccg cgtcgtcagc ggtccgtcgc caccgcggta
     5461 tg
//
LOCUS       DATLJT010000203         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1617910,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000203 DATLJT010000000
VERSION     DATLJT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617910"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..500
                     /locus_tag="VM910_04615"
                     /pseudo
     CDS             258..500
                     /locus_tag="VM910_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913673.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(588..821)
                     /locus_tag="VM910_04620"
     CDS             complement(588..821)
                     /locus_tag="VM910_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61863.1"
                     /translation="MDIAGITVHPDERWMQQIARNVTMEGCGALRDCRYLLHDRDTKY
                     TQSFRAQEGSLLSHCRQAGTRQGHPYVHPNGKV"
     gene            1170..2039
                     /locus_tag="VM910_04625"
     CDS             1170..2039
                     /locus_tag="VM910_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI61864.1"
                     /translation="MQTLKIGDVTITSIIERDGPWRRPEDMFPAYDPEVGRRHIAKLD
                     PIVFDVASGRMVITYQTFIVRTPKRTILVDTCTGEDKGYPPPMDFPKQPWLDGFRAGG
                     LRFEDIDYVFCTHLHIDHCGWNTLLRNGRWVPTFPKAKYVFHKLEYAAWEEATKRGDN
                     PPGNVWRFNCEPVVAAGQALLVDDDFQLDDTLWLTPTPGHSPCHCCVNIRSGGQRAVV
                     IGDLMHHALQCREPDWSTIFDWDRDQAARSRRRFLGEIAGTGAFVMPVHFPHPTAGLV
                     EADGDRFHYKFVR"
     gene            2125..2988
                     /locus_tag="VM910_04630"
     CDS             2125..2988
                     /locus_tag="VM910_04630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI61865.1"
                     /translation="MLTIDAQVHAYERNHPGRPWHAELTGPREVTGDQMVAAMDAVGV
                     DSAILVSPFTIYRYDASYALEVHERHSARFALVKPVDPANPSVAEVIADWKRTPGAVG
                     VRMLLVRGGYTEDPADPGLNRVLAEAGRLSLPVNLHIAGRLDQGLELIGRNPDTQIIV
                     DHLGLIQPHEPPVPVDPWAELPKVLTLAKMQHVAIKLSGACTLSREPFPYRDIWDPVC
                     RVIDAFGIDRCMWGTDWTRAVKFLNYAQGVDAFRVTDRISQSDKAKLMGGTLARIYGW
                     SPRQASISSLT"
BASE COUNT          599 a          905 c          925 g          614 t
ORIGIN      
        1 cctgcgtaag gaagcgccgg cgtgaagtgc gcagcacgtc attaatcatc ctgtgtgcga
       61 gcgtggatat tccctcgcca gacatgcacg acccctcccg gtgtggtccc cacgcaatcg
      121 tacatttctc gatatgagcc agcaagcgac tgagcgcgct cgaaggcagc ttatgatgtg
      181 gtggacggcg cccgctccgc ggcacgaagt gccgtagggt gatcgttgtt agacgaatca
      241 catgaggagc cgtccacatg caagttgcca cgatcggttt ggatctcgcc aagcacgtgt
      301 tccaggtcca cggcattgat gcggccgaga aggtcgtcgt caggaggcaa ctgcggcgta
      361 gccaggtgat ggcgttcttc gaagcgctgg cgccatgtct gatcggcata gaagcgtgcg
      421 ccacagcaca ctattgggca cgcgaactga cgaagcttgg ccatgaggtg cgcctgatgc
      481 cggcggaggt cataaaaccc taactcatgc ccgagtgctt gactgatgct gtcgacctga
      541 tcgtcgtgtc gactggcgcc tgtttcggga aaaatacctg accgctctca aactttcccg
      601 tttggatgga catacgggtg accttgtcgc gttccggctt gacggcaatg actgagaagt
      661 gagccttctt gagctcggaa ggactgggtg tacttggtgt cgcgatcgtg cagcagatag
      721 cggcagtctc gcagcgcacc gcagccctcc atggtcacgt tgcgggcgat ctgctgcatc
      781 caccgctcat ccgggtgaac cgtgatcccg gcgatgtcca cccggcggct ccatcgtgcc
      841 gatcagtctc tcgacgtacg ggttctgcca cggcgataga gccaaacgaa cagaaagcga
      901 tcggtattgc tgagttgtgg tcgattcgat acctgtcggc gaagcacgtt gagctgttag
      961 cgaaggatta ggatttctgc catgagcttc gttctcggca taaaagcgag gctatgatat
     1021 ggaggatcag tcggagtaac tcgatcatca agtggcaggg ttaccgagtc tctgcctcgc
     1081 gtctagccga atccaaattt tcggtacaca caggttggcc gccgcgacgt tagaaccgtg
     1141 atatcgttcc gccgtggaga tcaggcagta tgcaaacgct caagatcggg gacgtcacaa
     1201 ttaccagcat cattgaacgg gacggacctt ggcgccgtcc ggaggacatg ttcccggcgt
     1261 acgaccctga agtcggcagg cggcatatcg caaagctcga tccaattgta ttcgatgttg
     1321 cctctgggcg catggtgatc acttatcaaa ccttcatagt gcgtaccccc aagcgcacca
     1381 ttctggtgga tacctgtact ggggaggaca agggctaccc gccaccgatg gactttccaa
     1441 agcagccctg gctggacggt ttccgcgccg gcgggttgcg gttcgaggat atcgactacg
     1501 tattctgcac acacctgcac atcgaccact gcggctggaa tactttgctc cgcaacgggc
     1561 ggtgggtccc gactttcccg aaggcaaaat acgtgtttca caagctcgag tacgccgcct
     1621 gggaggaagc gactaagcgc ggagacaatc cacccggaaa cgtctggcgg tttaactgtg
     1681 agccggtggt cgcggccggt caggcgctgt tggtcgacga tgactttcaa cttgacgaca
     1741 cgctttggct cacccccacg ccgggccact cgccctgcca ttgctgcgtc aatatccgtt
     1801 cgggcggtca gcgtgccgtc gtcatcggcg acctcatgca ccatgcgtta cagtgtcgag
     1861 agcctgattg gtctacgatc tttgattggg accgcgatca agcggccagg tcgcgacgac
     1921 ggtttttagg cgagatcgcg ggaaccggcg cattcgtgat gccggtccac tttccacatc
     1981 cgactgccgg actggtcgag gcggatggag atcggttcca ctataagttt gttcgataga
     2041 ctgcgaataa tccagtagta gatagcgcga atgcagtcgt gccgagccgc gccggacgtg
     2101 ggcactcaaa gggagagcca aaatatgctg accatcgacg cgcaggtgca tgcctacgag
     2161 cgcaatcacc ctggccggcc gtggcacgcc gaattgacgg gaccgcgcga ggtcacgggt
     2221 gaccagatgg tcgccgcgat ggacgccgtc ggtgtcgaca gcgcaatcct ggtttcgccg
     2281 ttcacgatct accgctacga cgccagctat gcgctcgagg tgcacgagcg ccattccgcc
     2341 cggttcgccc tcgtcaagcc ggtcgatcct gccaacccgt cagtcgcgga ggtcatcgcg
     2401 gactggaagc gcactcccgg tgcggtaggc gttcgcatgc tgctcgtccg tggcggctac
     2461 accgaggacc ccgccgatcc gggcctcaat cgcgtgctgg cggaggctgg acgtttgtcg
     2521 ctcccggtca acctgcacat tgccgggcgg ctcgaccaag ggctcgagct gatcggccgc
     2581 aatcccgata cgcagatcat agtcgatcat ctcggcctta ttcagccgca cgaaccgccg
     2641 gtgccagtgg acccgtgggc cgaactgccg aaggtgctaa cgctcgcaaa gatgcagcac
     2701 gtcgccatca agctcagcgg cgcctgcacg ctatcccgcg agcccttccc ctacagggac
     2761 atttgggatc cagtctgtcg cgtcatcgac gcattcggga tcgaccgctg catgtggggc
     2821 acggactgga cacgcgcggt gaagttcctc aattacgccc aaggtgtcga cgccttccgc
     2881 gttacggacc gcatctcgca aagtgacaag gccaagttga tgggtggcac gctggctcgc
     2941 atctacggct ggtcgccgcg gcaagcatca atctcgagtc tgacgtgagt tgccgccgac
     3001 gaggtcatcg aatgattacg ctgctcgccg tcgccacggt cgc
//
LOCUS       DATLJT010000204         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1617987,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000204 DATLJT010000000
VERSION     DATLJT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617987"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VM910_04635"
     CDS             <1..1462
                     /locus_tag="VM910_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI61866.1"
                     /translation="AKAVMRHFQEANFLVPVRPLDGPSPHDVVWRPASNARILQVLKN
                     PAYAGAYVYGRRRRTPIGRRSGSRQGATEAVAIQDWPVCLKDAFPGYIDWEEFMANQR
                     RLADNLNHYDANHHGVPRQGNALLQGIAICGRCGRRMGLHYSGQNGNYPVYLCRADQH
                     QHGGPRCQEVRALSVDAAVERLLLEALTPDRLALAIAALGELENEARALERQWSLKRE
                     RARYEAERARRQYDAVEPENRLVARSLERVWEDKLRQAEQIDIEYESWRRQQPISLAA
                     ADRAQIISLGEDLPKLWDAASVVERKQILRLVVKEVVLDQRPESGQVWIRIIWQTGAV
                     SEHKVRRNVSSYRNYADVGTLEARVRDLVVARKMDREIARALNADGLLSAHGRPFSGG
                     EVHLLRKRWNIPTVKINQTEHNPQQWPDGTYSVQGAAEALSVSTQTVFKWLQKGRLSG
                     HQISKGMPWQINLSNTQIVELRTQVRRTTLSKKEAS"
     gene            complement(1472..2104)
                     /gene="istB"
                     /locus_tag="VM910_04640"
                     /pseudo
     CDS             complement(1472..2104)
                     /gene="istB"
                     /locus_tag="VM910_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(2104..3603)
                     /gene="istA"
                     /locus_tag="VM910_04645"
     CDS             complement(2104..3603)
                     /gene="istA"
                     /locus_tag="VM910_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI61867.1"
                     /translation="MLVVETVGRIRREHFVKGKSIKEIVRDLKVSRNTVRKVLRSGAT
                     CFDYAREVQPLPKLGPWRTELDRMLATNEAKSGRERLTLIRLFEELRGLGYEGGYDAV
                     RRCARTRRRARGAATAEAYVPLSFAPGEAYQFDWSHEIVVLNGVTTLVKVAHVRLCHS
                     RMPFVRAYPRESQEMVFDAHDRAFAFYRGACARGIYDNMKTAVESVFVGKDRAFNRRF
                     LRMCSHYLVEPTACTPGAGWEKGQVENQVGLVRERFFTPRLRFRTYEEMNGWLIDQCV
                     AYAKAHPHPECRDQTIWSVFEAERPSLIGYAGRFDGFHAVPAAVSKTCLVRFDNNKYS
                     IIAKALGRPVEVHAYADRIVIRQDGEIVAEHRRSFGRGQTVYDPWHYVPVLARKPGAL
                     RNGAPFKNWLLPGAIERIRRKLAAVDDGDRQMVDILGAVLTDGLGAVEAACVEALSAE
                     LHSSDIILNILARRRDASAAISILAPEALRLHHEPTADCARYDSLRRAV"
BASE COUNT          783 a         1218 c         1217 g          712 t
ORIGIN      
        1 cgccaaggcg gtgatgcgcc attttcaaga ggccaacttc ctggtgccgg tacgaccgct
       61 agacgggcca tcgccgcatg acgtcgtgtg gcggccggcg agcaacgcac gcatccttca
      121 ggttctgaag aaccccgctt atgcgggagc ctatgtttac ggccgtcgtc gacgcacgcc
      181 aatcggccgc cgttctggtt cacggcaggg cgcgaccgaa gccgtggcga tccaggattg
      241 gccggtttgc ttgaaggacg catttccggg atatatcgat tgggaggagt tcatggcgaa
      301 ccagcggcgg ctcgcggaca atctcaatca ctacgatgcg aaccaccatg gggtgccgcg
      361 ccagggcaac gcgttgctgc aaggcattgc tatttgtggc cgctgcggcc gccgcatggg
      421 gctgcattat tccggccaga acggcaacta ccctgtctat ctttgtcgcg ccgaccagca
      481 tcaacatggc ggaccgcgct gtcaggaggt gcgcgccttg agcgtcgatg cggcggtcga
      541 aagattgtta ctcgaagcgc tgacaccgga tcgcctcgct ctcgcgattg ccgctctcgg
      601 cgagctggag aacgaagccc gcgcgctcga gcgccaatgg tcgctcaagc gcgagcgagc
      661 gcgctatgag gcggagcggg cgcgccgtca atatgacgcc gtcgaacccg agaaccgtct
      721 ggtggcaagg tctttggagc gcgtatggga ggacaagctc cggcaagcgg aacagatcga
      781 cattgaatac gagagttggc gccgccagca gccgatatcg cttgccgcgg cggaccgcgc
      841 tcagattatc tcactgggtg aggatcttcc caaactgtgg gatgcggcga gcgtggttga
      901 gcgcaagcag atccttcggc tcgtcgtgaa agaggtcgtc ctcgatcagc ggcccgagag
      961 cggccaagtc tggatcagaa tcatctggca gaccggtgcc gtcagcgagc acaaggtccg
     1021 gcgcaatgtg agcagctacc gcaattacgc cgacgtcggg actcttgaag cacgggttcg
     1081 cgatctcgtc gtcgcgcgaa agatggatcg cgaaatcgca agggccctca atgcggacgg
     1141 cctactgtca gctcacggtc ggccgttcag cggcggtgag gtccatttac tacgcaaacg
     1201 ttggaatatc ccaacggtga aaatcaacca aacagaacac aatccccaac aatggccgga
     1261 tgggacatac tccgttcaag gcgccgctga ggcgctttcg gtttctacgc aaaccgtctt
     1321 caaatggctt cagaaaggac gattatctgg ccaccagatc agcaaaggaa tgccttggca
     1381 aatcaacctt tcgaacacgc agatcgttga gttgagaaca caagtacgac gcacaactct
     1441 ttcgaaaaag gaggcatcat gaggtcgtcg gctcgccgaa gactgaaggc cattcggcga
     1501 aagagagatt ggtggtgaca atgatcgagg tttgctcgta gagccggctg accaggtgga
     1561 acaacagctg gccgccggat tgggacaagg gcagatagcc gagttcgtcg agaatgagga
     1621 agtcgagccg ggtgaggcga tcggcgatcc ggccctgccg cccggcgcgc gcttcggcct
     1681 ccagtcgatt gaccaggtcg accgcggtat ggaagcggcc acgggcgccg gcgcggatgc
     1741 aggcgcgggc aatggctacg gcaagatggc tttttccggt gccggtaccg ccgaccagca
     1801 cggcattgcg ctggttctga aggaacgagc cagtggcaag atcacgcacg aggctctcat
     1861 tgattggcgt gtcgctaaaa tcgaagtcgg acagctcctt ggcgagcggc agccgagcaa
     1921 tggcgacctg gtatttgatt gaccgcgcct gcttctcggc aatctcggct tgcaatagat
     1981 cgccaataat ctgcggtacg gaatgttgcc gcttcagtcc attggcgagg atctcgtcga
     2041 aggcggcgcg catgccagcg agtttcagct cgcgcatcat ctcaatgatc tgatggcgtt
     2101 ccatcagacc gcccgtcgca gactgtcgta gcgggcgcaa tcggcggtgg gttcgtgatg
     2161 cagccgcagg gcctcgggcg ccaggatgga gatcgcggcc gatgcgtcgc gccgccgtgc
     2221 caggatgttg agaatgatgt cgctcgagtg gagttcggcg ctgagcgcct cgacacaagc
     2281 cgcctcgacc gcaccgagcc catcggtcaa taccgcgccg aggatgtcga ccatctgccg
     2341 atcaccgtca tcaacggcag cgagcttgcg acgaatgcgc tcgattgctc ccggcaacag
     2401 ccagttcttg aagggcgcgc cgttgcgcag cgctccaggc ttgcgcgcca ggaccggcac
     2461 gtaatgccag ggatcgtaaa ccgtctggcc gcgcccaaaa ctgcgccgat gctcggcgac
     2521 gatctcgcca tcctggcgga tcacgatccg gtcggcgtag gcgtgcacct ccactgggcg
     2581 acccagcgcc ttcgctatga tcgagtactt gttgttgtca aaacggacga gacatgtctt
     2641 cgagacggca gccgggacag cgtggaaccc gtcgaaccgc ccggcataac caatgaggct
     2701 tggccgctcg gcctcgaaga ccgaccagat tgtctggtcg cggcattcgg gatgaggatg
     2761 cgccttggca taggcaacgc actggtcgat cagccagccg ttcatctcct cgtaagtccg
     2821 gaaccgcagc cgcggggtga agaagcgctc gcgcaccagc cccacctggt tctccacctg
     2881 ccccttctcc cagcccgccc caggggtgca ggcggttggc tcgaccaggt aatgactgca
     2941 catccgcaaa aatcggcggt tgaatgcccg gtccttgccg acgaagacgc tctcgaccgc
     3001 tgtcttcatg ttgtcgtaga tgccacgcgc gcaggcgccg cgataaaaag cgaatgcccg
     3061 gtcgtgcgcg tcgaacacca tctcctggct ctcgcgcggg taagcccgca cgaacggcat
     3121 gcggctgtga caaagccgga catgggcgac cttgaccagt gtcgtcaccc cgttcagcac
     3181 aacgatctcg tggctccagt cgaactggta ggcttcgccg ggcgcaaagc tcagcggcac
     3241 gtaggcttcc gctgtcgctg caccacgagc ccggcgccga gtgcgggcgc agcgacgcac
     3301 cgcgtcatat ccgccctcat agccaaggcc gcgcagctcc tcgaagaggc gtatcagcgt
     3361 tagccgctcg cgtccgctct tcgcttcgtt ggtcgccagc attcggtcga gctcagtacg
     3421 ccagggcccc agcttcggca gcggctggac ctcgcgggca taatcgaaac aggtcgcacc
     3481 cgaacgcagc accttgcgca ccgtgttgcg cgacaccttc agatcgcgaa cgatctcctt
     3541 gatcgacttg cctttaacaa agtgctcgcg ccggatccgg ccgaccgttt ccacaaccag
     3601 catccccccg ccacccgctc gcgttccccc agcgggtagc ctgtgcaact cattgcaaag
     3661 ggggtcaaag ttggacgccg atcacccctc tacaggggtc aatcttccac gccgaaacac
     3721 accgggaaag tccgttgacc gaaccgacag cagccagtca actgtggcgg cggctaccgc
     3781 tcttcatgcc ccttttgtat ggaccggctg cctttcgcaa ctgagggcgt gctgtcggaa
     3841 gtcgctgata tgtatccggc ctgttgctcg gctcgcgggc cgtggccttg atgggagtac
     3901 gcacacacct ttgatctcat tagccacaga
//
LOCUS       DATLJT010000205         1890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4375152,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000205 DATLJT010000000
VERSION     DATLJT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4375152"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..>1890
                     /locus_tag="VM910_04650"
     CDS             146..>1890
                     /locus_tag="VM910_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVI61868.1"
                     /translation="MPKLFSDPEAIAEDIIRDVGTRLVVGLPLGLGKANHIVNALYAR
                     AAADRSIDLTFFSALTLEKPKPGNLLEARFIGPVIDRLFGGYPDLAYVEPLRKGELPS
                     NIRVIEFFFLAGRWLHVPYAQQHYVSANYTHAVPYLLTEGLNVVTQLVAKRVVDGVTR
                     YSLSCNTDTTLDVLRARAEGRASFKLIGQVNSELPFMPGAGDLPGEEFSAVLDGAKVD
                     FPLFAPPAEPITDTKYAIGLHSAGLVRDGGTLQIGIGQVGDALAQSLILRHRDASRYM
                     EILKRLAPATELATLQTGPFEKGLYGLSEMVTEAFVGLIEAGILKREVDGAILHGAFF
                     LGPKSFYRALREMTPQQLARVQMMPVSFTNELYGDEETKRAARLDARFVNSAMMATLL
                     GGAISDGLEGGRVVSGVGGQYNFVAQAFALSGARSILALDATRQGRAKAQSNIRWIYG
                     HETIPRHLRDVFVTEYGVADLRGKSDADVIAAMLGVADSRFQDELARAAKDAGKLPKG
                     YQIPAACRENLPERVARALKPAREAGLLPSFPFGSDFTDVEQRLIPALQTLQDAQHTP
                     LRLAGLLWQGLARTP"
BASE COUNT          330 a          605 c          596 g          359 t
ORIGIN      
        1 ccacattaaa actgcaagtg ccatcaactc gcccgcttgc tgttgcgcct ccgtcacaca
       61 ccattcatgg ttccaaatgc gctatagcgg gaatatgccg gattcgtttt gaggcgccgg
      121 ctgccttgca cggggttgaa atgcgatgcc gaagctgttc agcgatcccg aggcgatcgc
      181 ggaggacatc atccgcgatg tcggcacaag gctcgtcgtc ggcctgccgc tcggactcgg
      241 caaggccaac cacatcgtca atgcgcttta tgcgcgggct gccgccgatc gatccatcga
      301 tctgacgttc ttctcggcgt tgacgctgga gaagccgaag cccggcaatc tcctggaggc
      361 tcgcttcatc ggcccggtga tcgatcgcct gttcggcggc tatccggatc tcgcctatgt
      421 cgagccgctg cgcaaaggcg agcttccctc caacattcgc gtcatcgaat tcttttttct
      481 ggcggggcga tggctgcacg ttccctacgc gcagcaacat tacgtctcgg cgaattatac
      541 ccatgccgtg ccctatctcc tgacggaggg gctgaacgtc gtcacccagc tcgtcgccaa
      601 gcgcgtggtc gatggcgtca cccgttacag cctgagctgc aacaccgata ccacgctcga
      661 tgtgttgcgg gcgcgcgcag aaggccgtgc ctcgttcaag ctgatcggcc aggtcaattc
      721 ggaactgccg ttcatgccgg gcgcaggcga tctgcccggc gaggaatttt ccgcggtgct
      781 cgacggcgcg aaggtggatt ttcccctgtt tgcgccgccg gccgagccga tcacggacac
      841 caaatatgcg atcggattgc actcggcggg cctggtgcgc gacggcggca cgctgcagat
      901 cgggattggc caggtcggcg acgcgctggc acaaagcctg atcctgcgcc atcgcgacgc
      961 ctcgcgatac atggagatct tgaaacgcct tgcgcccgcg accgagcttg cgacgcttca
     1021 aacagggccg ttcgagaagg gcctctatgg cctcagcgag atggtcaccg aagcattcgt
     1081 tgggctgatc gaggccggca ttctcaagcg cgaagtcgat ggcgcaatcc tgcatggcgc
     1141 cttctttctg gggccgaaat cgttctatcg cgcgttgcgc gagatgacac cgcagcagct
     1201 cgcacgtgtc cagatgatgc cggtgtcctt caccaacgag ctctacggtg acgaggagac
     1261 aaagcgcgcc gcgcgcctcg atgcccgctt tgtcaatagc gcgatgatgg cgacgctgct
     1321 gggaggggcg atctcggacg ggctcgaagg cggccgggtc gtgagcggcg tcggcggcca
     1381 gtataatttc gtggcccagg cctttgccct gtcgggcgca cgctcgatcc tggcgctcga
     1441 tgcgacgcgg caggggaggg ctaaagctca atccaacatc cgctggatct atggtcacga
     1501 gaccatcccg cggcatttgc gcgacgtctt cgtcaccgaa tatggcgtcg ccgacctcag
     1561 gggaaagtcg gatgccgacg tcatcgcggc gatgctgggc gttgccgact cgcgctttca
     1621 ggacgaactc gcgcgcgcgg ccaaggacgc cggcaagctg ccgaagggct accaaattcc
     1681 cgccgcttgc cgcgaaaatc tgccggagcg cgtcgcgcgc gcactcaaac cggcgcgcga
     1741 ggcggggctg ttgccgtcct ttccgttcgg cagcgatttc accgacgtcg agcagcggct
     1801 gatcccggcg ctgcagacct tgcaagacgc gcagcacacg ccgctccggc tggccggcct
     1861 tttatggcag ggacttgcgc gaacgccgga
//
LOCUS       DATLJT010000206         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2767309,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000206 DATLJT010000000
VERSION     DATLJT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767309"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VM910_04655"
     CDS             <1..180
                     /locus_tag="VM910_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI61869.1"
                     /translation="TVGFGLSALGGWGAGVALDAAGGPGQPAGWSALFALLAAGVLFG
                     PIALRWSRQGHAMAS"
     gene            431..691
                     /locus_tag="VM910_04660"
     CDS             431..691
                     /locus_tag="VM910_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61870.1"
                     /translation="MRRREFITLLGGAAVAWPLAARAQQAAIPVIGFLSSSSPDLFAS
                     RLRAFHQGLSETGYVASYRRNAARRQRVPLKVHSAQIGERSS"
     gene            688..954
                     /locus_tag="VM910_04665"
     CDS             688..954
                     /locus_tag="VM910_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61871.1"
                     /translation="MKKLLIGVSLVAALATTTPAHAYYLRDCLRGAPCPGFYGQTPRY
                     YGPPRRYYGPSPYGPSPYYGPSPYYGPSPYYGPPRRYYGPPGYY"
     gene            1575..2798
                     /locus_tag="VM910_04670"
     CDS             1575..2798
                     /locus_tag="VM910_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61872.1"
                     /translation="MFKPKIWLRLGALAVASSATALPKPILATETQEYSKEAARQPGT
                     AGPVLAQSGRRGAGGEGGEGGEGGEAGSSRPAVAPKVAPQQQQKSGAKQRRGLGGEGG
                     EGGERGFGPRSKRAGTRGRQSGEGEEEEEEEEGERGVNTRYIFGFTEGADTERAREKE
                     FENDTVGRLGKRSGTYTALQNKSEVEFGVTDNVLVEFGLFGSYHRIQDVPDFDDRNGA
                     RFDGAFAELKYRFLDRDVHGFGMAFSVEPEWHRYSELTGRFENSYAVELELLVDKELI
                     PGTLFLAGNLVYEPEAVLAKEFDPDTGQFTKWERESNFQALGAISGAVTPNLFVGAEV
                     RYLAKYEGNFLNHLEGRALYVGPTLYARLSPKSTITLAYSWQVAGKATEEPDQKLDLL
                     NFERQQFRVRWVSEL"
     gene            3053..3442
                     /locus_tag="VM910_04675"
     CDS             3053..3442
                     /locus_tag="VM910_04675"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVI61873.1"
                     /translation="MRWFRSRSRLGAYLALFALAFQLAVSFGHVHLERIAPPSVGTGA
                     SALAGAQPSADDPNAPSKPAGREHPADDHCPICTLIHLAGALVPAEAPSLPLPTVFGR
                     LRLELAAEFDLTASHAALFHARAPPIA"
     gene            3575..>4207
                     /locus_tag="VM910_04680"
     CDS             3575..>4207
                     /locus_tag="VM910_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HVI61874.1"
                     /translation="MSIRSRRLLLLSSSALVLLCFAGIASSQTPEAPPETPPATTTTP
                     QTPPTTTPAPETPAPQPSQPAPETPQAEPGTPPAVTIPQVTVEAPKPRPARRTAPAAP
                     ARTTTPAPPPPAPPPTTTPPTAPSTTTTAGQPAAPSPFQPPPSLTTLNSEQIQASQSN
                     SLGGLFFTMPGATSAGLAPGTQRPVLRGLDDFRVRVQENGVGTMDVSDLGQ"
BASE COUNT          826 a         1287 c         1301 g          793 t
ORIGIN      
        1 acggtgggat tcggcctttc ggcgctgggt ggctggggcg ccggtgtagc gctcgatgct
       61 gcgggagggc cggggcagcc ggcaggatgg tccgccttgt tcgcactgct cgcggcagga
      121 gttcttttcg gtcccatcgc cttgcgatgg tcgcggcaag gacacgcgat ggcatcatag
      181 ccaaggtcga tggggcggtt acttggtgtg ttcgttactc ccagggaggc ggctggctag
      241 tctagggcgt gtactcatta acgcgcaatg tcgggttttg gggggctaag cggaaatctt
      301 gtgctcggcc agagcactac cggttttgac ccatctgcga catcagcgcg caatttttgc
      361 tgtgatgcac agcgacgttc ttgcacaacg gcgtgctaat gtgcggccct aagcctgagg
      421 aggggcgccc atgaggcgcc gcgagttcat cacgctgctc ggcggtgcgg cggtcgcctg
      481 gccgctcgcg gcgcgcgcgc agcaggctgc gataccggtg attggatttc tcagcagttc
      541 atcgcctgac ctattcgcga gccgtctgcg cgcgtttcac caaggcctaa gcgagaccgg
      601 ctacgtcgca agttatcgcc gtaatgcagc taggcggcaa agggtgccgc tcaaggtgca
      661 ttccgcacaa ataggagaga gatcctcatg aaaaagcttc tgatcggcgt aagcttggtc
      721 gctgcgctgg cgacgacaac accagcgcac gcctactact tacgcgactg ccttcgcggc
      781 gcaccctgtc cggggttcta cgggcagacc ccgcgatact atggtccacc tcgacgatac
      841 tacggcccgt cgccgtatgg tccgtcgccc tactatggtc cgtcgcccta ctatggtccg
      901 tcgccctact atggtccgcc ccggcgatac tatggtccac caggctacta ctaagacgaa
      961 ttcggcttcg gtgatccggc gatcatcggt ggcattattg gagcaatcat tgccgaagcc
     1021 attgccgggc aacgatgacg ccacaatggc cccggcaacc ctacattagc cggggcattc
     1081 ttttaattgg ccgcccgaaa atttctcttc gcgccaaggc atggcgaagc gtctagccat
     1141 tgttgcgcac tccacccgtt cgattgacgg cgtaaccgcc gccaatgaaa gcacagcttt
     1201 cattttcagt taactcgcca tttacgcttc tgccgagaac gactcgcgct gccgatttga
     1261 gagtgagaga atcagcgtgg ctcggcttcg gtgatgaatt ggggcgctca agctccaatc
     1321 tcagcccaag caccgagtcg agccaccact ccccttcacg ccgccctgcc aagtgttctg
     1381 atgctgagca tccggatggc gctcatatac gcgagtgcgt ggatggtcag aattggatgg
     1441 aatccgctca gattcctgta atggttctaa ttaaggacgc tcacctttga gatattccaa
     1501 aagaaaacgc tcggtcggaa cattcgagca tgagatgatt acggtcgggc ggtttgggct
     1561 aaaggggggt gttaatgttc aagccaaaga tttggctacg gctgggagct ttggccgtgg
     1621 caagttcagc gacggcattg cctaagccaa tcctcgcaac agagactcaa gagtacagca
     1681 aagaagcagc caggcagccg ggtactgccg gacccgttct cgcgcagagc ggccgccgag
     1741 gcgcgggagg agaagggggc gaaggtggtg agggcggcga agctggctca agccggcctg
     1801 ctgttgcgcc caaggttgct ccacaacagc aacagaaatc cggagccaag caacgaagag
     1861 gcctcggagg tgaaggcgga gagggcggcg agcgcggctt cggaccccgc agcaagcgcg
     1921 ccgggacccg cgggcggcaa tcaggagaag gagaagaaga agaagaagaa gaagaagggg
     1981 agcgaggtgt caacacgcgc tacatcttcg gattcaccga gggcgcggat acggagagag
     2041 cgcgggaaaa agaatttgaa aacgacacgg tagggagatt gggaaagcgc tcaggaacgt
     2101 acactgcctt gcaaaataaa tccgaggtcg agtttggcgt gacggataat gtgttggtcg
     2161 agttcgggct gtttggctca tatcaccgga tacaggatgt tcctgatttt gatgacagga
     2221 acggcgctcg tttcgacggc gcgttcgcag aactgaaata ccggttcctc gatcgcgacg
     2281 ttcatggctt cggaatggcg ttttccgtcg aaccagagtg gcaccgctat tccgagctca
     2341 cgggaagatt tgagaatagc tacgctgtgg agctcgaact tctcgtcgat aaagagctga
     2401 tcccgggaac actcttcctc gctggcaact tagtgtatga gccggaggcg gtgctcgcca
     2461 aagaattcga tccggacaca gggcaattca ccaaatggga gcgcgagtcc aactttcagg
     2521 ccctgggcgc aatttctgga gccgtcaccc caaacctgtt cgttggcgcc gaagtgcggt
     2581 atttagccaa atacgaaggt aatttcctca atcacctaga aggtcgagcg ctctatgtcg
     2641 gaccgacact ctacgcgcgc ctatcaccca aatcgaccat aacgctcgca tactcgtggc
     2701 aggtggcggg gaaagccacg gaagagccgg atcaaaagct ggacctgctg aacttcgaac
     2761 ggcagcagtt tagggtccgc tgggtctccg aattgtagac gtaagtgcta cccggtgtag
     2821 tgctcttctg cgcaagaaag gctagcccgc ttcagtcggc ggcccttttt tattcgcctt
     2881 gagcaaccgc agctgtggaa cggccgctgg acctcgcaat gttgattttt ggcaacacac
     2941 cagatgaatc gcaacgcggc ccaattgtcg gcaatgttac actgtaacaa gctctcgcgc
     3001 gtgccggcag aatggttctg ccgtggcatg agacgcgcga agcgtgacga ccatgcgttg
     3061 gttccggtcg cgttcgagat tgggggcgta tctggcgctg tttgcgctgg cgttccagct
     3121 cgcagtttcg ttcggccacg tgcatctcga acgcatcgcg ccgccttcgg tgggcacggg
     3181 cgcgagcgcg ctcgccggcg cgcagccgtc ggccgacgac cccaacgcgc cttccaaacc
     3241 ggccggtcgc gaacaccccg cggacgacca ttgcccgatc tgcacgctga tccatctcgc
     3301 cggcgcgctg gtgccggccg aggcgccgtc gttgccgctg ccgaccgttt tcggccggtt
     3361 gcggctcgag cttgccgccg aatttgatct gacggcatca cacgccgcgc tgttccacgc
     3421 gcgcgcacct cccatcgcct gacatcgact gtggtctcgg agcgccctcg cgccagcgag
     3481 ggctgggccg ataccccatg cggcgctgct ttcgccgtca tgcgtttgat tgtcggggtt
     3541 gacggatcgt ttccgtcagg aatgggggcg tgtgatgtct atccgtagca ggcgtttgct
     3601 gctgttgagc agctcggcgc tcgtgctgct ctgcttcgcc gggatcgcat cgtcgcaaac
     3661 gccggaagcg ccgccggaga ctccgccggc gacgacaacg acaccgcaga ctccgccgac
     3721 cacgactccc gcgccggaaa ctcctgctcc gcaaccgtcg cagccggctc ccgagacgcc
     3781 gcaagccgag cccgggaccc cgccggcagt cacgattccg caagtcacgg tggaagcacc
     3841 gaaaccgcgg cccgcgcggc ggaccgctcc cgcggctccc gccaggacga cgacaccagc
     3901 gccgccgcca cccgccccgc cgccgacaac gactccacca acggcgccga gcacgaccac
     3961 caccgcggga cagccggccg cgccgtcgcc gttccagccg ccgccgtcgc tcaccaccct
     4021 caacagcgag caaatccaag cgagtcagag caacagcctg ggcggactct tcttcaccat
     4081 gcccggcgcc acctccgccg gtctcgcacc cggcacgcag cggccggtgc tacgcggcct
     4141 cgacgatttc cgcgtgcgcg tgcaggagaa cggcgtcggc accatggacg tttccgactt
     4201 gggccag
//
LOCUS       DATLJT010000207         1903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_9930,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000207 DATLJT010000000
VERSION     DATLJT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9930"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VM910_04685"
     CDS             <1..333
                     /locus_tag="VM910_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhopane nucleosidase"
                     /protein_id="HVI61875.1"
                     /translation="EKRALYLKTGAIAVDMESHIVAAVGLAHDVPVAAIRVITDPAKR
                     ALPVSAVAAMRPNGTTNIAAMLRSVLTRPRELPALFQTALDARAARATLVRGRHLLGP
                     AFTLPLTT"
     gene            344..538
                     /locus_tag="VM910_04690"
     CDS             344..538
                     /locus_tag="VM910_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61876.1"
                     /translation="MFKSAAEEISQWADQCRRWAGDARTRDQKLMLQNLERLLSQAAL
                     DAEGALELDCVLRPLAPSKS"
     gene            624..1655
                     /locus_tag="VM910_04695"
     CDS             624..1655
                     /locus_tag="VM910_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI61877.1"
                     /translation="MTRAVAPQALGYIGVRAKALDDWASYGAGLLGLQRVDQSCSTIA
                     FRMDDHKQRVVVHADGGEGIGFFGWEVADATSLEALAAHLDNGGIAVAHGSRALADER
                     HVADLIVLSDPVGNRLEFFHSAQTTTEAFRPGRCISGFRTGPLGLGHVVLNVDRPDTL
                     ARLMAFYRDMLGFRLTDYYSEPFIARFLHVNPRHHSLAFIQTGKNAVHHVMMELFSFD
                     DVGQGYDLALAEEGRLAVTLGRHTDFITSFYTWTPSSFMVEYGWGARTIDVEHWQAYE
                     RKEGPSLWGHDRRWLSAADNAKARAQRLANAADSNRRPVQVIEGNYQLMPGICPWWDS
                     LTSRGGAQK"
     gene            1675..>1903
                     /locus_tag="VM910_04700"
     CDS             1675..>1903
                     /locus_tag="VM910_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI61878.1"
                     /translation="MVQPTAGRNTHTRRIAEFVSQLSYDRIPAPVRERLKLLILDSLG
                     CAIYGAQLEWCRILRHTLEQSDETRDASIWGT"
BASE COUNT          346 a          616 c          579 g          362 t
ORIGIN      
        1 gagaagcgcg cgctctatct gaaaaccggc gcgattgcgg tggatatgga atcgcacatc
       61 gtggcggccg tcggcctcgc ccatgacgtc ccggttgccg cgatacgggt catcaccgat
      121 cccgccaaac gcgccctccc ggtttctgcc gtcgccgcga tgcggcccaa tggcacgacc
      181 aatatcgccg ccatgctgcg ctcggtgctc acgcggccgc gcgaattgcc ggcgctgttt
      241 cagaccgcgc ttgatgcgcg cgcggcgcgt gcgacgctgg tccgcggccg ccatttgctc
      301 ggccccgcct tcacactacc gcttacgact tgagaggcgt gcaatgttca agtctgcagc
      361 tgaagaaatc agccaatggg cggatcaatg ccgccgttgg gcaggcgatg cgagaacccg
      421 cgaccaaaag ctgatgttgc agaacctgga gcggcttttg agccaagccg cgcttgacgc
      481 cgagggcgcg cttgaactcg actgcgttct gcgcccgctc gctcccagca aatcgtaaag
      541 aaaacagtac gaaatcggac ttggcagccg tgatggcgcg gttgtgcccg tcagtgtaaa
      601 agcattccgg cgagaccctc aagatgacgc gcgctgtggc accgcaagcg ctgggttata
      661 tcggcgtccg cgctaaagct ctggacgact gggccagcta tggcgccggt ctgctcggct
      721 tgcagcgcgt ggatcaatcg tgctccacga tcgctttccg catggatgac cacaagcaac
      781 gcgtcgtggt ccatgccgat ggcggcgaag gcatcggctt cttcggttgg gaagtcgccg
      841 acgccacgtc gctcgaggcc ttggcggccc atctcgacaa cggcggcata gccgtcgcgc
      901 acggcagtcg ggcgctagcc gacgagcgac atgttgcgga tttgatcgtg ctctccgacc
      961 ccgtcggcaa ccggctggaa ttctttcaca gcgcgcaaac cacgacggag gcgttccgac
     1021 ctgggcgttg tatttccggc tttcgcaccg gtccgctggg cttgggtcac gtcgtgctca
     1081 acgttgaccg gccggatacg ctggcccggc tgatggcctt ctatcgcgat atgctcggct
     1141 ttcgcctgac cgactattat tccgaacctt tcattgcccg tttcctccac gtcaatccgc
     1201 gtcatcacag cctcgccttc attcagaccg gaaagaacgc agtccatcac gtcatgatgg
     1261 agctgttcag cttcgatgac gtcggccagg gctacgatct ggcgctcgcc gaggaaggcc
     1321 gccttgcagt aacgctcggc cgccatactg atttcatcac atcgttttat acctggacgc
     1381 cgtcgtcttt catggtcgaa tacggctggg gcgcgcgcac gatcgacgtc gagcactggc
     1441 aggcttatga gcgcaaagag ggcccgagcc tatgggggca cgaccggcgc tggctgtcag
     1501 cggcggataa cgccaaagcg cgcgcacagc gccttgccaa tgcggcagac agcaatcgcc
     1561 ggccggtcca agtgatcgag ggcaactatc agctcatgcc cgggatctgt ccgtggtggg
     1621 acagcctcac atcgcgcggc ggcgcgcaaa aatagtcgat ccgcgagagc gcttatggtt
     1681 cagcctacgg ctggtcggaa cacccacacg cgccggatcg ccgaattcgt ttcgcaatta
     1741 agttacgacc ggattcccgc tcccgttcgc gagcgcctca agctccttat tctcgactcg
     1801 ctcggctgcg cgatctacgg cgcccagctg gaatggtgcc gtatcctgcg gcacaccctc
     1861 gaacaaagcg acgagacgcg agacgcgtcg atctggggca ccg
//
LOCUS       DATLJT010000208         5431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3916001,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000208 DATLJT010000000
VERSION     DATLJT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3916001"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VM910_04705"
     CDS             <1..126
                     /locus_tag="VM910_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="HVI61879.1"
                     /translation="VERFFAWIQWQRRILVRWEYYAQNFLGFVQLACLVVLFRRF"
     gene            147..872
                     /locus_tag="VM910_04710"
                     /pseudo
     CDS             147..872
                     /locus_tag="VM910_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2387..3631
                     /locus_tag="VM910_04715"
     CDS             2387..3631
                     /locus_tag="VM910_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI61880.1"
                     /translation="MAPKRKREKGEKQRLTDAIVRRHPLPKHGKAITVDADVPGFGAR
                     VTANGARSFILRYTTRAGRERTYTIGDATVWRTTDARDKARELRREIEDGGDPLGDIE
                     EERAAPTMLDLIDRFRAEHLPRKRPSTRDDYERTLRLHIVRHFGQHTKVADVRFEDVD
                     ALHRKVTAAGNPYQANRTIALLSKMFSLAQRWRMLDTNPCKGIERNTEYHRRRYLSGD
                     ELVRLTQALAKHPDQQVANAVRIMLLTGARRGEVLGMRWDDVKLGTGIWSKLPSSTKQ
                     KEHHQVPLSAPALQLLSEIDKRQRRAGKRPKFVFPSNGATGHYVEIKKSWRQITKAAG
                     IEGLRIHDLRHSYASQLVSGGASLPLIGALLGHSNPATTARYAHLFHDPLRAATEKVG
                     AVFSAAGRPAMPPTPIRRGRRP"
     gene            3628..4032
                     /locus_tag="VM910_04720"
     CDS             3628..4032
                     /locus_tag="VM910_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61881.1"
                     /translation="MKKTRQRAGIDRGIRLIMLAKWRQAFERLRAVPGPPSAFKQLRE
                     LTERMHAGTDIEEAQQHLQKMALEQAAALANPREQSKRRAAAIKREIDARAAKLKRCG
                     VSNPVEQARAEAAKRHGYASEQALKKWLRRNR"
     gene            complement(4149..4346)
                     /locus_tag="VM910_04725"
     CDS             complement(4149..4346)
                     /locus_tag="VM910_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61882.1"
                     /translation="MNAAQDKLVVASLWSIFHAAEKSVRRAIAIAALLRALRDAMLLG
                     HLVVAKERLISDDNALGVRFQ"
     gene            4442..5296
                     /locus_tag="VM910_04730"
     CDS             4442..5296
                     /locus_tag="VM910_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61883.1"
                     /translation="MAQRSLDLIERMYVAAEAAQPITGRGIGYKLFTAGLIPSMATKE
                     MQRVYRLLKVARERGDIPWEWIVDETRAIERTSTWANPAEYARCVARSYRRDFWDQQL
                     IRCEVWSEKGTVRGVLAPVLDHYAIGFRVMHGFSSATTIYDVSQDDDGRELIVLYVGD
                     FDPSGMFMSKEDLPARLSDYDGDHIELRRVALTREHVNGLPSFPVTDKRKDPRYKWFR
                     ANYGDRCWELDAMDPNDLRDCVEAATKELIEPVAWKRCEIVNHAEQESLKTILEKWTP
                     RRCSGAAG"
BASE COUNT         1127 a         1643 c         1657 g         1004 t
ORIGIN      
        1 gtggagcgct tctttgcttg gattcaatgg cagcgccgca tcctcgtccg ttgggaatac
       61 tacgcccaga acttcctcgg tttcgtacag ctcgcttgcc tcgttgtcct cttcaggcga
      121 ttttgagata ggttctagtc agttggcaag gatggcggac aactctccta acgcttgcga
      181 ttatcttgct cgcggtatgc ttcccaattc acgcattggg cctgcgcgag cgatcaacga
      241 ctacggaagg acaaacggga tcgcgggtcg gaaaggcaag cacagcggcc ttaaggcgtg
      301 cactgcttca tccctcgttc tgggctctcc tggtgacgtt tacgattcac tccacacttg
      361 ccaccgtaat cgtgtttcac atcatgcctc tcctgcacga gaggggtttc tccgagctaa
      421 cgatcacctt tgcctactca ttgataggac ctgcacaggt aggtagtcgt attgcattgc
      481 tggcaacccg ccgatatttc tcgatggaaa caattggaat tatcaccgta tcggccctgc
      541 ctctctcatt ggcgctactg cttgtactgc cgcagatcga gatagcgcct tgtctaatgc
      601 ttgttctgta cgggacagca aatgggctcg tgaccatcct ccggggaacg gcaattcccg
      661 acctaattgg gtccgaaggc tacggctcga tcaatggaac gattactctt gtcgcacgca
      721 ttggcgcggc actggcgcca attgcggtcg cgctcgcatg ggacaaattc ggaagctacg
      781 ggccggttgt ctggggattg ttcgcgctgg ccgtcatcgc agccgtaagc tatggcgtcg
      841 ccatctatgc tgccgaaggg ggaaaacctt aagcttagag acgacagcga taatgatagc
      901 ggtagatttc acgaaatccg agcttgcggc cgagcagaaa ttccgggctg cgtgtcgggg
      961 tgtggcgcga aggatcgtcg cccgtggggc cgagacgccg cagagctaaa cgacgcgcgg
     1021 atggcaacat caaggcctga tttccaatat acgtttcatt atgactgtct tgatacgcgt
     1081 tgaaggacgc aattcgcaaa atctccgccc cggccaagtg ttcccagacg gccccccgtc
     1141 cgggtccgag gcttcgccgg tgcagacgcc ctgcactgaa attccccgca tgcccccacg
     1201 tacccccaca cgcgccttca cagtatcatt tcactgcccg ggtgttcggg ggtcaagtgg
     1261 cttggaagtc ggctccgggt cacactcggc ccttttcaat tcgtttcgct tcccctcgct
     1321 tccgggtatc cggtggcgcc atcggtaacc agcggcgcaa tgcgcaagaa gcgcggtggc
     1381 gtattgctct cgaccataat gacgcggtga gttccgccgc cttcagcccc gacgggtcgc
     1441 gcatcgtagc ggcgtcacgg gacaggaccg cccgcatctg ggacgccgcg agtggcaaag
     1501 agatcgcggt cctgcgccac atccacggcg tttccaccgg cggccatgaa agcgggcttc
     1561 gcttcgctct acggatacac gagcgacaac caaggcattc gacatgctca ccttaacgag
     1621 ccctcttcga aacctgacga ggccgaggcg ctgttcatga tcggctcgtg tgctgcattc
     1681 gtttcgtatc tgatcaacaa agcccggctc cttccgcttg ctcaaacgta agctttttgc
     1741 gctcggcata cgggtcgtct gcgatgctca cgacaatgct ccaaacgtcc ggcggcccgc
     1801 ttccgtcatc ctcaacgttg cgacttccct cttgcgcccg ccaacgatga cacgctgcgc
     1861 cgtcgccgtg gcgagcccgg cgcgcacgag attgaccatc tgatcgatgc tgatcccatg
     1921 cgagcattac cgccttggca ccctcctgag ggcagtgggc cagcaattcg agcgcgcggc
     1981 ggcgggaagg gttgggatgg cgacgggcgg cgaaggcgcg tctagaaggc gaggtaggcg
     2041 actggcggca accggcaaca ttaacattag tggatgccat cgaacaaagg tggtctgttt
     2101 gtcggaataa gccgctgatg gcttgtcgga atggcgttgc tgctgtgtgt taagtgtttg
     2161 agagtattgg tgggcgcacc aggactcgaa cctgggaccc gctgattaag agtttcatcc
     2221 gcgaccattc cgcaagccct tgcaccgcca gcaattcccc cgagaaccgc agagaaacgc
     2281 ccgccacatc gtggtttctc gttgcaacgt cgggacaggt ccattacatc tctggacacg
     2341 acatgccgtc aacgcgacgg gcatgggacg ggcatggaga ttcccagtgg caccgaagcg
     2401 gaagcgggag aaaggcgaga agcaacggct caccgacgcc atcgtgcggc ggcatccact
     2461 tcccaagcat ggcaaggcca tcaccgtcga cgccgacgtt cccggcttcg gtgcccgcgt
     2521 cactgccaac ggggcgcggt ccttcatcct cagatacacg acccgcgccg gacgcgagcg
     2581 cacctatacc atcggcgacg ccaccgtgtg gcgcaccacg gacgcccgcg acaaagcgcg
     2641 cgagctgcgg cgcgagatcg aggacggcgg cgatccgctg ggtgacatcg aggaggagcg
     2701 cgccgccccc accatgctcg atctgatcga tcggtttcgg gctgagcatc taccacgcaa
     2761 gcgcccatcc acccgcgacg actatgagcg cacgctgcgg ctacacatcg ttcggcattt
     2821 cggccagcat accaaggtgg ccgatgtccg tttcgaggac gtcgatgcgc tccaccgcaa
     2881 ggtcaccgct gccggtaatc cctatcaagc gaacaggacc atcgcgctgc tgtcaaagat
     2941 gttcagcttg gcgcagcgct ggcgcatgct cgatacgaat ccgtgcaagg ggatcgagcg
     3001 caacaccgaa taccatcggc ggcgctatct cagcggtgac gagctggtgc gcctcaccca
     3061 agcgctggcc aagcatcccg accagcaggt cgccaacgcg gtcaggatta tgctgttgac
     3121 tggcgctcgg cgcggtgaag tgctcggcat gcgctgggat gacgtcaagc tcggcacggg
     3181 catctggtcg aagttgccgt cctcgaccaa gcagaaagaa caccatcagg tcccgttgag
     3241 tgcgcctgcg ctacagctgc tgtcggagat cgacaagcgg cagcggcgcg cgggcaagcg
     3301 ccccaaattc gtcttcccca gcaatggagc gactgggcac tacgtcgaaa ttaagaagag
     3361 ctggcggcag atcaccaagg ctgcgggaat cgaaggcctg cgcatccatg acctgcgcca
     3421 cagctacgcc agccagctgg tgagcggcgg tgcctcgctg ccattgatcg gcgcgttgct
     3481 tggccacagc aacccagcca cgacggcgcg ctacgcgcat ctattccacg acccgttgcg
     3541 ggccgcgacc gagaaggtcg gcgcggtctt cagcgctgcc ggaaggcccg ccatgccgcc
     3601 gacgccgatc cggcgcggga ggcgaccatg aagaaaaccc gccagcgcgc cggtattgat
     3661 cgaggcatca ggctcatcat gctcgccaag tggcggcagg cgttcgagcg gctgcgcgcg
     3721 gttcccggcc cgccttcggc gttcaagcaa ttgcgtgagt tgaccgagcg catgcatgcg
     3781 ggcaccgaca tcgaggaggc gcagcagcac cttcaaaaaa tggctctaga gcaggcggcc
     3841 gcgctcgcaa atccgcgcga gcagagcaaa aggcgagcgg cggccatcaa gcgcgagatc
     3901 gacgcacgcg ctgccaaact caaacgatgc ggtgtcagca atccggtgga gcaggccagg
     3961 gctgaagcgg ccaagcgaca cgggtacgcc agcgagcaag cgctcaaaaa gtggctgcga
     4021 cgcaaccgct gagggggaca aaaatgtccc gcgactgggt tcgtctcatg cccctaatag
     4081 atcgtcatcc tgaagaaagg gatgacggtc caatggacac ctctactaag cggcgacttg
     4141 atccgcgctc actggaagcg caccccgagc gcattgtcgt cgctgatgag acgttccttc
     4201 gcgacgacaa gatggccgag aagcatggcg tctcgaagcg cacgcagcag cgcggcgatc
     4261 gcgatggcgc gccgtacaga tttttcggcg gcgtgaaata tcgaccagag cgacgctacg
     4321 accaatttat cttgggcagc attcaaatac gccagtcccc gccgcacaag cgaatacaaa
     4381 agaagcggag gcggacgcga tgacggagca ttttgaaacc cgttccaaaa aaggtcgcgg
     4441 catggcgcag cgatccctcg atctgatcga gaggatgtat gtcgcggccg aagccgcgca
     4501 gccgatcacc ggtcgcggca tcggatataa gctgttcacc gctggactga tcccctcgat
     4561 ggcaacgaag gaaatgcagc gcgtgtatcg tctgctcaag gtggcgcgcg agcggggcga
     4621 catcccgtgg gaatggatcg ttgatgagac ccgcgccatt gagcggacat cgacatgggc
     4681 gaatcctgcc gagtacgccc gctgtgtcgc gcgatcctat cgccgcgact tttgggatca
     4741 gcagctgatc cggtgcgagg tttggagcga gaaaggcact gtgcgcggcg tacttgcccc
     4801 tgtgctcgat cattacgcga ttggcttccg cgtgatgcac gggtttagca gcgccactac
     4861 catctacgac gtttcccaag acgacgatgg ccgagaattg atcgtgctct acgtcggcga
     4921 cttcgatccg agcggcatgt tcatgtcgaa agaggaccta cctgctcggc tctccgacta
     4981 tgacggcgat cacatagagc taaggcgagt tgcgctgaca cgagaacatg tgaacgggct
     5041 tccgtcgttc cctgtgacgg ataagcgcaa agacccgcgc tacaaatggt tccgggccaa
     5101 ttacggagac cgttgttggg aactcgatgc gatggaccca aacgatcttc gcgactgcgt
     5161 tgaggcggca accaaggaat taatcgagcc cgttgcgtgg aaacgctgcg agattgtcaa
     5221 ccacgccgag caggagtcgt tgaagacgat ccttgaaaag tggacgccgc gccgttgctc
     5281 gggcgcggca gggtgaacat gcgcatccac tcggattgca cggcgggcga gagcagtggc
     5341 gacgaaggag aaagagtggg ttgaaaaagt gtggccgcgc cgagggacag cgcggccggg
     5401 gaaggaaatg gtggctacga caccaaccat c
//
LOCUS       DATLJT010000209         7451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3916171,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000209 DATLJT010000000
VERSION     DATLJT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3916171"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VM910_04735"
     CDS             complement(<1..914)
                     /locus_tag="VM910_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61884.1"
                     /translation="MASHIGRRKFLATLGGAAVAWPPAARAQSPKMLRVGYSGMLPRD
                     APHYAAFERRMAELGYQPGRNFTFEYIQAPSIEGYEVTYRELAARKVDVLLAAGNEPA
                     LRAARSAAGATPIVFIALDFDPVEKGYVASLSRPGNNSTGLFVSQLELAGKRVEFLRK
                     TFPNARHIGILWDATSREQADAAAAVAGHLGFEPRLLEAIGQPPNYTAALMPMDQWPG
                     EPIMIPASPLALRDRATIVRLLLDRRAPSICAFREVMEAGALFSYGVNLVDVFRDVAA
                     AVDQVARLGNAGEIPMRSPSHFHTAVNV"
     gene            1483..2691
                     /locus_tag="VM910_04740"
     CDS             1483..2691
                     /locus_tag="VM910_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61885.1"
                     /translation="MSGKKTVLGMALVAIAIASASVASAAEPIRIGELNSYTAEADFT
                     RPYRNGWIMAIEEINAAGGVLGRTLEVVFRDDGAEPGKAVTAAEELTTKLNVQLLAGT
                     YFSHVGVAVTNYAKQRKKLFVATEPMSEDITWKQGNRYTFRLRPNTYNQSAMLAEEAA
                     KAPAKRWAIVAPNFKFGQDAVAAFKKILKQKRPDVEFVEEQWPPLAKIDPGATVQALL
                     QARPEALYCALFSQDLSAFVREGKVRGLLTDKLTVVGLLTGEPEYLDPMGAEAPQNWI
                     VTGYPWYAINDPKHQKFVDGYKAKFKENPFMGSLIGYVSATAIKSMIEKAGATDQDKL
                     IQAMEGLTFDSVVGPISFRALDHQSTMGTWVGRTAVKDGKPLMVDWHYREGAQYFPPD
                     SEIKALRPAE"
     gene            2982..4847
                     /locus_tag="VM910_04745"
     CDS             2982..4847
                     /locus_tag="VM910_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61886.1"
                     /translation="MAGLAGGQLGFYFVQFLTGLASASSLFLVASGLSIIFGVTRVVN
                     FAHGSFYMLGAFIAYSLVNLLPGGSLGFWGGVLLSALAVGVIGVLMEILILRRIYASP
                     ELFQLVATFGVVLVVQDVALLIWGAEDKLGPRAPGFEGAIDVFGAKVPTYDLMMVALG
                     LSVLAGLWLLFSRTRWGAVVRAATEDREMLGALGVNQALLFTAVFFLGSVLAGLGGAL
                     QIPRASVSHHMDLAIIASVFVVVVVGGMGSIIGAFLAAAIISELNAFGILVLPKISLF
                     LMYLVMAVVLIIRPWGILGTQEAEAGRDHSADETLAILPSSSVRWTMAAILLLLVVLP
                     LVVQEFYLVLLTEVFIFALFAASLHFIMSNGGMVSFGHAAYFGLGAYAVAMLVVYGGA
                     SMEPAFLGAPLAAGLGAAVFGWFCVRVSGVYMAMLSLAFAQIVWSVVFQWDQITGGEN
                     GMVGVWPAEWASSRVVFYYVALIVCGGSILVLWRMLVAPIGYGLRAGRDSALRTESIG
                     INLQAQRWLAFIIAGVFAGVAGGLYAFSKGIVDPSTVSISRSIDALMIVLLGGLQSLV
                     GPMVGALTFVGLQDRLATLEFWRLIFGMIIIAICVVMPKGIAGSTRALFVQSRRK"
     gene            4858..5634
                     /locus_tag="VM910_04750"
     CDS             4858..5634
                     /locus_tag="VM910_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61887.1"
                     /translation="MGVLEVSGLSKSFGGVRALIDVSFSVDTGELLAMIGPNGAGKST
                     CFNCINGQVRADAGSVRFQGQEIVRRLPRHIWRLGVGRTFQVTATFGSMTVAENVQLA
                     LISRSRRSRALWSLATRLYRDEALRLLEAVGMQDQAARRCGVLAYGDLKRVELAVALA
                     HSPTFLLMDEPTAGMAPRERVELMRLTAQIVGQRGVSILFTEHDMGVVFENASRVIVL
                     NRGRVIAEGPPMVVRSNRTVQDIYLGAGDWATRGRTPFNA"
     gene            5627..6328
                     /locus_tag="VM910_04755"
     CDS             5627..6328
                     /locus_tag="VM910_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61888.1"
                     /translation="MLEVRNLHAHYGRAHILDGVSLAVGAGEVGVLLGRNGAGKTTTL
                     KSIMRLLRSSRGEIVFAGRSIMALQPFQISRLGLGYVPEDRRIFKELSVAENLDVARQ
                     PPRKGAPTWTVDRIFKLFPNLAERYRSSAKVLSGGEQQMLTIARTLMGNPRAILLDEP
                     SEGLAPVIVEQMARAIQELKAQGVTVLLSEQNLYFSAIVADRAFIIEKGAIRYAGSMQ
                     DALNNAAIRTAYLMV"
     gene            complement(6373..7200)
                     /locus_tag="VM910_04760"
     CDS             complement(6373..7200)
                     /locus_tag="VM910_04760"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVI61889.1"
                     /translation="MVGSLSQGQVEHFRREGYAFPFDAISSEEAAACVSKLDSYDEIL
                     GEECQKQLKIKAHVAAPWIIKLSRNPNILNAVESVIGPNILLFGASMFSKRARDSRFV
                     SWHQDAAYYGLDPQEEVTCWVGLTDADMENGCMRVMPRSHKAPDAVHEETYDPQNLLG
                     RGQTIRGIDDSKAVYMPVRAGQFSMHHERTIHGSMPNPSARRRVGIAFFYMPTHVRST
                     IGRRSATLVRGTDSYGHWDPEPMPKTDLDPECMTFLRQSWARYRDPDVPQAAVSKAS"
BASE COUNT         1375 a         2163 c         2327 g         1586 t
ORIGIN      
        1 ttgacgttga ctgcggtgtg aaaatgcgat ggcgaccgca tcggaatttc gcctgcgttg
       61 ccgagcctgg caacttggtc caccgctgcc gccacatccc ggaagacgtc cacgaggttg
      121 acgccataac taaagagcgc ccccgcctcc atcacctcgc gaaaggcaca tatcgacggg
      181 gcgcggcggt ccaacagtag gcgcacaatg gtcgcgcgat cgcgcaaggc gagcgggctt
      241 gcaggaatca tgattggctc gcccggccac tgatccatcg gcatcagggc agccgtatag
      301 ttcggcggct gcccgatggc ttccagcaac cgcggctcga agcccaggtg tccggccaca
      361 gcggcggcgg catcggcttg ctcccgcgat gtcgcatccc aaagaattcc gatgtgacgt
      421 gcgttcggga aagtcttgcg caagaactca acacgttttc ccgccaactc gagctggctc
      481 acgaagagcc ccgttgaatt gttgccgggg cgtgacaagc tcgccacata gcccttctca
      541 accggatcga agtcgagcgc aatgaagaca attggcgttg cgcctgcggc cgaccgcgcc
      601 gcccgcagcg ccggctcgtt ccccgccgcg agtaggacat ccaccttgcg cgcggcaagc
      661 tcgcgatagg ttacttcgta cccctcgata cttggcgcct ggatgtactc gaatgtgaaa
      721 ttgcgacccg gctggtaacc gagttcggcc atgcgcctct cgaaagccgc ataatgcggc
      781 gcatcgcgcg gcagcatgcc ggagtaaccc acgcgcagca tcttgggaga ctgcgcgcgc
      841 gccgcgggcg gccacgcgac tgccgcgccg ccgagcgtcg ctaagaattt tcgtcgtccg
      901 atatggcttg ccaagtgacc ctccgattgg gggtcattct tgaaatggag ggatgatacc
      961 acccttctat cgcgaggtca gtgaataagg gcgataggca agtcgggtga atggtcccga
     1021 aagaccgggt gggatcaaaa tcggtctttg cggtataccc gctgcgtgac cgcttttccc
     1081 caagaacaga cattcgtcgg cacacataga acgtccgttt cgtgccaaga cgcgacataa
     1141 cgcggtgcag caaattggca ccgctcccca tgccatttcg cgtcctgttt agctttcgtc
     1201 gcgttctttc gaccagcgtg actagacccg tggggtagag tgactgggta acgcccggcc
     1261 gccgcgaacg cgggactacc ttgtactcca acatggttgg tggcaaatct attgtgagga
     1321 aagccgaatg aatctgcggc ggacgatcat cgtcttctga ccctgctgat cgcgccgcca
     1381 ttctcgtttt tgcaccgagc gcgcgtccgc tttaaaactc cggtagcacc gaatccacgc
     1441 gcggttgaac gaagtcggag ccaagacgta gggagatgca aaatgagtgg caaaaaaact
     1501 gtactgggta tggcactggt tgcaatcgcc atcgcgtcgg cgagcgtggc cagtgccgcc
     1561 gaaccgatcc ggattgggga actcaacagc tataccgcgg aggcggactt cacgcggccg
     1621 taccggaatg gctggatcat ggcgatcgag gagatcaatg ccgccggcgg cgtcctcggt
     1681 cgtaccctcg aggtggtctt ccgagacgac ggcgccgagc ccggcaaggc cgtaaccgcg
     1741 gcagaggaac tgacgactaa gctcaacgtg caactcttgg cgggcaccta cttctcccac
     1801 gtcggcgtgg cggttaccaa ttacgccaag cagcgcaaaa agctgttcgt cgccaccgag
     1861 ccgatgagcg aggacatcac ctggaaacag ggaaatcggt acacgtttcg gctaagaccg
     1921 aacacctata atcaatccgc catgctcgcg gaggaagccg cgaaagcacc agcgaaacgt
     1981 tgggctattg tcgcgcccaa cttcaagttc ggccaggacg ccgttgcggc cttcaaaaag
     2041 atactgaagc aaaagcggcc cgatgtggag ttcgtcgagg agcagtggcc gcctctcgcc
     2101 aagatcgacc ctggcgcgac ggttcaggcc ttgctccaag cgcggcccga ggccctctat
     2161 tgtgcgctgt tcagccagga tctgtcggca ttcgttcgcg agggcaaggt gcgaggcctg
     2221 ctgaccgaca agcttacggt tgtgggcctg ctcaccggcg aacccgagta cctggacccg
     2281 atgggcgcgg aagcgccaca gaactggatc gtgactggct atccttggta tgcaatcaac
     2341 gaccctaagc atcagaaatt cgtcgacggt tacaaggcta aattcaagga aaatcccttc
     2401 atggggagcc taatcgggta cgtctcagca accgcgatca aatcgatgat cgagaaggcg
     2461 ggggcgacgg accaggacaa gcttattcag gcgatggaag ggctgacctt cgactcagtt
     2521 gttggcccga tctcgttccg cgccctcgac catcaatcga ccatggggac gtgggtgggc
     2581 cggaccgccg tgaaggacgg caaaccgttg atggtcgact ggcattatcg ggaaggcgcg
     2641 caatactttc cgcccgacag cgaaataaag gcgttgcgac ctgcggagta gcgatcagca
     2701 ccggggcgat gacccgcccg ctggcgccgc gccagcgtgc aactcgcatg cgcggttgag
     2761 gacgtttgat gcctgcgcca tgcactcggt ctgttccctc cccccttatc ggggagggtt
     2821 ggcgaggcac cccggcgcgc ttgcgacccc cgaggggctg gcgtaactct gcgcggcatc
     2881 gcggccattc aggacctcac ccccaatcgc ttcccacaag cgggagagga gtcgagtgac
     2941 cttgttgctc gtgttcattc aagctcatcg agaacgctag cgtggccgga cttgcggggg
     3001 gtcaattggg attctacttc gttcaattcc tgaccggctt ggcgagcgcg tcttcgcttt
     3061 ttctggtcgc gtctggcctg tccatcattt tcggcgtaac ccgagtcgtc aatttcgccc
     3121 acggctcgtt ctatatgctg ggggcattca tcgcgtattc attggtcaat cttctgcccg
     3181 gcggctcgct ggggttttgg ggcggggtcc tgctcagcgc gcttgccgtc ggagtgattg
     3241 gggtgctgat ggaaatcctc atcttgcgga ggatctatgc atccccagag ctgtttcagc
     3301 tcgttgcaac cttcggtgtc gtactcgtgg tccaggatgt cgcgctcctc atttgggggg
     3361 cggaagacaa gctcggcccg cgcgccccgg gattcgaggg cgcaatcgac gtcttcggcg
     3421 caaaggttcc gacctatgat ctcatgatgg tcgcgctcgg cctttcggtt ctggccggtc
     3481 tttggctcct gttcagccgg acgcgttggg gcgcggtggt gcgggccgcc accgaggacc
     3541 gggaaatgct cggcgcgctc ggagtaaacc aggcacttct tttcaccgct gtgttctttc
     3601 tggggtccgt gctggccgga ctgggcggtg cattgcaaat tccccgggca tcggtcagcc
     3661 accacatgga ccttgccatc atcgccagcg tcttcgttgt tgtcgtcgtt ggcgggatgg
     3721 ggagcatcat tggagctttt ctggcagccg cgatcatcag cgaattgaat gcctttggaa
     3781 ttctcgtgct tcccaaaatt tcactctttc taatgtacct ggtaatggcc gtcgttctga
     3841 tcatccgtcc atggggaatt ctcggcacgc aggaagccga ggccggccgc gaccactcgg
     3901 cggacgaaac gctggctatc ctgccgagca gctcggtccg ctggaccatg gcggcgatcc
     3961 tgctgcttct tgtcgtgctg ccgctcgttg tgcaggaatt ctacctggtg ctgctgacgg
     4021 aagtcttcat atttgcccta ttcgctgcga gcctgcattt tatcatgagc aacggcggaa
     4081 tggtctcgtt tggtcatgcg gcgtacttcg gactgggcgc gtacgcggtc gcgatgcttg
     4141 tcgtttacgg cggcgcgagc atggagccgg cttttctggg cgcccccttg gcggcgggat
     4201 tgggtgccgc ggtgttcggt tggttctgcg tacgcgtctc cggcgtgtac atggccatgt
     4261 tgagcctcgc ctttgcccag atcgtctggt cggtcgtttt tcaatgggat cagataacgg
     4321 gaggggagaa tggcatggtg ggcgtctggc ctgccgagtg ggcgagctcg cgggttgtgt
     4381 tctattatgt agcgctcatc gtctgcggcg gctccatcct ggtgctgtgg cggatgttgg
     4441 ttgccccaat cggatatgga ctgcgcgccg gccgggattc agccttgcgc actgaatcga
     4501 tcgggatcaa cctgcaggcc cagcgctggc ttgcgttcat cattgctggc gtttttgcgg
     4561 gggttgccgg gggcctctac gcattttcca agggtatcgt cgatccgtcg acggtgagca
     4621 tctcgcgctc aatcgatgcc ttgatgatcg tcctgctcgg tggacttcaa agcctcgtcg
     4681 ggccgatggt cggcgccttg actttcgtag gcctgcagga tcggttggcg acgctagagt
     4741 tttggcggct catcttcggc atgatcatca tcgccatttg cgtggtgatg ccgaaaggca
     4801 tcgccggctc gacgcgggca ttgttcgtgc agagccggcg caaataaggt tgcaggcatg
     4861 ggcgtgcttg aggtctcggg actgagcaaa tcgttcggag gagtgagggc cttgatcgac
     4921 gtcagcttct ccgtcgatac gggcgaactt ctggcgatga tcggaccgaa cggcgccggc
     4981 aaatctactt gcttcaactg catcaacgga caggttcgag ccgatgcggg ttccgtccgc
     5041 ttccagggac aagaaatagt caggcgtttg cctcggcata tctggcgact gggggtcgga
     5101 cgcacctttc aggtgacggc aaccttcggc tcgatgacgg tcgccgagaa tgtgcagttg
     5161 gcgttgatat cgcgttcgcg gcggtcgcga gcgctgtggt cattggcgac acgcctgtat
     5221 cgcgacgagg cgcttcggct gctcgaagcg gtcgggatgc aggatcaggc ggcgcgacgg
     5281 tgtggcgttt tggcctacgg cgatctcaag cgagtcgagc tggcagtcgc tctcgcgcat
     5341 agtccgacct tcctgctcat ggacgagccg acggcgggca tggctcctcg ggaacgcgtc
     5401 gagttgatgc gattgacggc gcagatcgtt ggccagagag gagtaagcat cctctttacg
     5461 gagcacgaca tgggcgtggt cttcgagaat gctagccgcg tcatcgttct caatcgcgga
     5521 cgggtaatcg ccgagggccc gcccatggtg gtgcgcagca atcgaacggt tcaggatatt
     5581 tacttgggcg cgggagattg ggcgacacga gggcgaacgc cgttcaatgc ttgaagtcag
     5641 aaatctccac gcccactacg ggcgcgcgca tattctcgac ggtgtctcgc tcgcggtcgg
     5701 agccggcgag gtcggtgtgt tgctgggacg gaacggggcc ggcaagacaa cgacactcaa
     5761 aagcatcatg cggttgctac gctcttcgcg tggcgaaatc gtcttcgcgg gacgctcgat
     5821 catggccttg cagccgttcc agatttcccg gctgggcctg ggctacgttc ccgaggatcg
     5881 gcggattttc aaggagctca gcgttgccga gaacctcgat gttgcgcgtc aaccgcctcg
     5941 taaaggcgcc ccgacatgga cggtcgatcg gatattcaaa ctgttcccga acctggcgga
     6001 gcgttaccgg agctcggcca aggtgctgtc gggcggtgaa cagcagatgt taacgattgc
     6061 tcgaacgctg atgggcaatc ctcgggccat tctgttggat gagccatccg agggactggc
     6121 gccggtcatt gttgagcaga tggcacgcgc aattcaagaa ttgaaagccc aaggcgtaac
     6181 cgtgctgctg tccgagcaga acctctactt ctcagctatc gtggccgacc gggctttcat
     6241 catcgagaaa ggcgcgatcc gttatgcggg atcgatgcag gacgcgctga acaacgccgc
     6301 catccgcacg gcctacctca tggtttagcc gaccggacta gtttgcgggc atctcgtcgg
     6361 taccctgctt tatcaggatg ccttcgacac tgccgcctgc ggcacgtcgg ggtcgcgata
     6421 acgcgcccag ctttgccgga gaaatgtcat gcattccgga tccagatcgg tcttgggcat
     6481 gggctccgga tcccagtgtc cgtaagaatc agtgcctcgc acgagcgttg cgctacggcg
     6541 accgatggtg gagcgcacat gcgtgggcat ataaaagaag gcgatgccca ccctgcgccg
     6601 cgccgatgga tttggcatcg acccgtggat ggtgcgctca tgatgcatgg agaactgtcc
     6661 tgcgcgcacc ggcatataga cagctttgct gtcatctatg cctcggatgg tctgaccgcg
     6721 ccccagcagg ttttgcgggt cgtaagtttc ttcatgcacc gcatcgggtg ccttgtgtga
     6781 tcgcggcatg acgcgcatgc atccattctc catgtccgca tccgtcaagc cgacccaaca
     6841 ggtgacttcc tcttgcggat cgagcccgta ataggccgca tcctgatgcc atgacacaaa
     6901 gcgactatcg cgcgccctct tggagaacat cgatgcgccg aacagcagga tgttgggccc
     6961 gatgaccgat tcgactgcat taagaatgtt cgggttgcgc gagagcttaa tgatccaggg
     7021 ggccgcgaca tgggctttga ttttaagctg tttctggcat tcttcgccga gaatttcgtc
     7081 ataactatcg agcttcgaaa cgcaggctgc ggcctcctcg ctgctgattg catcgaaggg
     7141 gaaggcatag ccctcccgac gaaaatgctc gacctgccct tggctgagac tacctaccat
     7201 cttggcctcc tttaactcag ttctcttcgc cggctccggt caattcaacc gcatcgccgt
     7261 ggtgctgagc tcgctgggat agagcactac ccgtttgcta tcctgaattt ctcttacggg
     7321 caaggatcgt gaggcattcc tgcggggttt cgcggaactt agtcgacgcg atcaacaggc
     7381 ggctgggctt gggcctgcgg tagggcattc atgccgcaca acgaagcaag gcggcaggtc
     7441 gcccaaatcg t
//
LOCUS       DATLJT010000210         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1848415,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000210 DATLJT010000000
VERSION     DATLJT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848415"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VM910_04765"
     CDS             <1..1186
                     /locus_tag="VM910_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HVI61890.1"
                     /translation="PIITFINKMDRESRDTFELMDEIEKTLALDTTPMTWPVGRGRDF
                     VGTYDVRDGGIRLLEGGGAKTGAAQQIEIADLARYNANLDVAALQEELALVTEACKPF
                     ELEAFREGHLTPVYFGSALRNFGVGDLLEGLGQFAPPPRAQDSDLRKVEASEPRMSAF
                     VFKIQANMDPNHRDRIAFARLCSGKLNRGMKAKLVRTGKSMPLASPQFFFAQDRSVAD
                     EAFAGDVVGIPNHGTLRIGDTLTEGEDLTFVGVPSFAPEIVRRVRLTDAMKAKKLKEA
                     LQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYALPVEFEVSEFQLARW
                     ISSDDRKKLDAFIAANASSIADDVDGDPVFMAKNDFYLGYTMERAEGIAFANVKDVKK
                     KG"
     gene            1285..1887
                     /locus_tag="VM910_04770"
     CDS             1285..1887
                     /locus_tag="VM910_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61891.1"
                     /translation="MMRRVLFGIVVSAAVASADAGQARRHQINPIPFSHDPCSVLSRR
                     PCTPTYCSPLQHGPCIPEIFYPYGENLQVTVETVPRKEDEAKYQRPDHDLDNIGDLFA
                     ELRSCWSPPPPDVAREGMQMSVRFSFKKSGEMIGPPRMTFATAGVPADVRNTYLKAIN
                     ASLDGCIPLKFTDGLGGALAGRPIAIRYVDNRELGKSSDK"
     gene            2056..>2520
                     /locus_tag="VM910_04775"
     CDS             2056..>2520
                     /locus_tag="VM910_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HVI61892.1"
                     /translation="MGLVVMIAGLVLFFGAHTLTTQRDLRARFIVSLGEGGYKAAYAL
                     VSLAGLVLIVWGFAYYRATGWIQIWSPPRALKHVNVALMLPAVILVVAAYIRGRIYAT
                     LKHPMLAGVKLWAFGHLLANGDLGGIILFGSFLAWAVYDRISLKYRADPGAPP"
BASE COUNT          456 a          773 c          793 g          498 t
ORIGIN      
        1 tccgatcatc accttcatca acaagatgga ccgcgagagc cgcgacacct tcgagctgat
       61 ggacgagatc gaaaagacat tggcgctcga caccacgccg atgacctggc cggtcggccg
      121 tggccgagac ttcgtcggca cttatgacgt ccgcgacggc ggcatccgcc tgctcgaagg
      181 cggtggcgcc aaaaccggcg cggcgcagca gatcgagatt gccgatcttg cgcgttacaa
      241 cgccaatctc gatgttgcgg ccctccagga agagcttgcg ctggttaccg aagcctgcaa
      301 gccgttcgaa ctggaggcgt tccgggaggg tcacctgacg ccggtctatt tcggcagcgc
      361 actacgcaat ttcggcgttg gcgatctgct cgaagggctc ggccagttcg cgccgccgcc
      421 gcgtgcgcag gacagcgatt tgcgcaaggt cgaggcgtcg gagccgcgca tgagcgcctt
      481 cgtcttcaag atccaggcca atatggaccc gaaccaccgc gaccgtatcg cgttcgcacg
      541 gctctgctcg ggcaagctca atcgcggcat gaaggcaaag ctggtgcgca cgggaaagag
      601 catgccgctg gcgagcccgc agttcttctt tgcccaggat cgctcggtgg cggacgaggc
      661 ttttgccggc gacgtcgtcg gcatccccaa ccacggcacg ctccggatcg gcgacacgct
      721 gaccgaaggc gaggatctga ccttcgtcgg cgtgccgagc tttgctccgg aaatcgtgcg
      781 ccgggttcgc ctcaccgatg cgatgaaggc gaagaagctg aaagaagccc tgcagcaaat
      841 gtcggaagag ggcgtcgtgc aggtgtttcg tccgcgcgac ggggcgccgg ctctggtcgg
      901 tgtggtcggc ccgctgcagc tcgacgtcct caaagcccgc cttgatgccg aatatgcgct
      961 gccggtcgag ttcgaggtat cggaattcca gttggcgcgg tggatctcat ccgatgaccg
     1021 caagaagctg gacgccttca tcgccgcgaa tgcatccagc atcgccgacg acgtcgacgg
     1081 cgaccccgtc ttcatggcga agaacgactt ctatctaggt tacacgatgg aacgcgccga
     1141 ggggattgcg tttgccaacg tcaaggacgt gaagaagaag ggataacctc gcttccgaac
     1201 acgcgcggtt gtgaacggga gcgggcttga tgccgcccag caggcccgcc attctcttga
     1261 tgtgtcgcct tcgaggtgat tttcatgatg cggcgcgttc tgttcggaat tgttgtctcg
     1321 gccgcggttg cgagcgccga cgccgggcag gccaggcggc atcagatcaa tcccattccg
     1381 ttctcgcacg atccctgcag tgtgctttcc cgaagaccct gcacgccgac ctattgcagt
     1441 ccgctgcaac acgggccttg cattcccgag atcttctatc cctatggcga gaacctgcag
     1501 gtcaccgttg agaccgttcc caggaaggag gatgaggcca aataccagag gccggaccac
     1561 gatctcgata acattggcga tctctttgca gagttacgct cctgctggtc gccgccgccg
     1621 ccggatgtcg cgcgcgaggg catgcagatg tcggtgcgct tcagcttcaa gaagtccggt
     1681 gagatgatcg gcccgccgcg tatgaccttt gccaccgcgg gagtgcctgc ggatgtccgc
     1741 aacacttacc tcaaggcgat caatgcctcg ctcgatggct gcatcccctt gaagttcacg
     1801 gacggtctcg gtggcgcgct ggcgggacgc ccgatcgcga tacgctatgt cgacaaccgc
     1861 gagctcggga agtcctctga caagtagtcg ttctcgttcc gaggcaatga ggccgatgaa
     1921 ttctgctcat ggggtctcgc gttcgaaaca gcgcgtgcgt gcctcctcac gacgacgttc
     1981 tggatgatgc attgccgcgc acaacccaaa aatgatacgc gttgagccgg aaagtggccc
     2041 agcagggaga gggaagtggg actggtcgtc atgattgcgg gcctggtgct gtttttcggg
     2101 gctcatacgc tcaccactca acgcgacttg cgcgcgcgct ttatcgtctc cctgggagag
     2161 ggcggctaca aagccgccta cgctctggtg tcactcgcag ggcttgtcct gatcgtgtgg
     2221 ggttttgcgt attatcgcgc aaccggctgg atccagatct ggtcgccgcc acgcgcgttg
     2281 aaacacgtca acgtcgcgct gatgctgcct gccgtaatcc tggtcgtggc ggcctatatc
     2341 cgtggccgga tctacgcgac gctgaaacac ccgatgctgg ccggtgtcaa gctttgggcg
     2401 ttcgggcatc tgctcgcgaa cggcgatctc ggcggcatca tcctgttcgg ctcgttcctc
     2461 gcgtgggcgg tgtacgaccg catttcgctg aagtatcgtg ctgaccccgg tgccccgcca
//
LOCUS       DATLJT010000211         4490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_10434,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000211 DATLJT010000000
VERSION     DATLJT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10434"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1599
                     /locus_tag="VM910_04780"
     CDS             292..1599
                     /locus_tag="VM910_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI61893.1"
                     /translation="MARTVRDANLETRTARLRLGIRSEPYWRGLEKGFALGYRRRAKG
                     GTWLARRRPDGGGYGEHKIGTSDDLQDADGVAILDYGQAQKAARTWWRAELRREEGHD
                     TREGPFTVRDAIDDYLRAYEQRGGKAVYDARRAAETHILPALGSLQVAKLTVKRIEDW
                     HRGLATKPALARSKLGRKPNHRKADKSADGVRKRRATANRILTVLKAALNHAWKSGPV
                     ASDDAWRRVKPFRAVETARVRYLSEAECVRLVNACEPAFRNLVRGAMLTGCRYSELAS
                     LCAADFNADAGVLIVRESKAGKPRHVVLTDEGQRLFARLTAGKLGNDSVFVRRDGGVW
                     RKSQQMRPMLDACNQAKIKPAVSFHVLRHTHGSTLAMRGVPMGVIAEQLGHADTRMTE
                     KHYAHLAPSYVADTIRAHFPTLGIAEESMVTPIRDRNRHIRRG"
     gene            complement(2574..2795)
                     /locus_tag="VM910_04785"
     CDS             complement(2574..2795)
                     /locus_tag="VM910_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61894.1"
                     /translation="MPNSLALETNHWFGPEDVAALTTAFEAAVGKLGLVDRSDPTTVT
                     FAKLIIELAKGGEREPERLCDSALKRLSK"
     gene            complement(3582..3983)
                     /locus_tag="VM910_04790"
     CDS             complement(3582..3983)
                     /locus_tag="VM910_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI61895.1"
                     /translation="MGAQQNRRAVLIVEDDAELREFAARLLEDGELDTIECESAEAAL
                     ATEMLIRGQDVAMIFADIRLPGVMDGIDLAREVRMRSPFLPVILTSGHPPEGQLPLGV
                     DFMPKPWQPLNLLVAAKQALSVSVRLASKAS"
BASE COUNT          875 a         1434 c         1409 g          772 t
ORIGIN      
        1 acggagctga ccaagcagcc gtaggcgatc cgccgccgcg cttggaagtc gcgcctgccg
       61 cgtagccttc gagccgaggc cgaatggaaa agcgagcgtt ggcgcgccct acgggactcg
      121 aacccatgtt tccgccgtga aagggtcaat acctgtatcc cgacgtacac aggcgaccgc
      181 ccagaaacat agatatagca ggcatttctt gcatcttctg ttcgtcatgg tacactgccg
      241 ttcgtccaca gatactcccc aaaagctccc caaaaattcg gagacgggaa tatggcacga
      301 acggtccgcg acgcgaatct cgaaacccgc accgcgcggc ttcgacttgg aatccgttcc
      361 gagccctact ggcgcggttt agagaagggt tttgcgcttg gctaccgccg gcgggccaaa
      421 ggtggcacct ggctcgcgcg gcggcggccg gatggcggcg gctatggcga acacaaaatc
      481 ggaaccagcg acgatttgca ggatgctgac ggcgtggcca ttctcgacta cggccaggcg
      541 cagaaggcgg cgcgaacatg gtggcgcgcg gagttgcgtc gcgaggaagg ccacgacacg
      601 cgggaaggtc ctttcactgt cagggacgcg attgacgact atctcagggc ttacgagcag
      661 cggggcggga aggccgtgta cgacgcccgc cgggcagcgg agacgcacat cctgccggcg
      721 ctcggctctc tgcaagtcgc caaactcacg gtcaagcgga tcgaagattg gcaccgcggc
      781 cttgccacaa agccagcgct cgcgcgctcc aagctcggcc gcaagccaaa ccaccgcaag
      841 gccgacaaga gtgcggacgg ggttcgcaag cgtcgcgcca ccgcgaaccg cattttgacg
      901 gtcctcaagg ccgcattgaa ccatgcttgg aaatccggcc ccgtcgcaag cgatgatgct
      961 tggcgccggg tcaagccgtt ccgcgcggtc gagacggcgc gcgtgcgcta tctttccgag
     1021 gccgaatgcg tgcggctggt caacgcctgc gagcccgcct tccgcaatct cgtccgcggc
     1081 gcgatgctga cgggctgccg gtattccgaa ttggcctcat tatgcgcggc agatttcaat
     1141 gctgatgctg gcgttttgat cgttcgcgag agcaaggcgg gcaagccgcg ccacgtcgtc
     1201 ttgacggacg aaggacaaag gctctttgcg aggctgacgg ctggcaagct cggcaacgat
     1261 tcggtcttcg tgcgacgtga cggtggcgta tggcgcaagt cgcagcaaat gcggccgatg
     1321 ctagacgcct gcaatcaggc gaagatcaag cccgccgtgt cgttccacgt ccttagacac
     1381 acgcatggct caacgttggc catgcgcggc gttccgatgg gtgtcattgc cgaacaactc
     1441 gggcacgccg acacgcgaat gaccgagaag cactacgcgc atcttgcccc gagctacgtc
     1501 gccgatacca tccgcgcgca tttcccgacg ctgggcattg ccgaggaaag catggtgacg
     1561 ccgatccggg acaggaaccg acatattcgt cgcggctgaa acggcactgg ctcggtcaag
     1621 ctcccaagcg atatgctgcg gcgcggcccg cccgcgccga cgaggtgatc gaatgaagcg
     1681 tgcgccgatg tctgctcatg gcacgaagcg gcggttatca aatgtccgct tttgcgccgc
     1741 ttgtgggggc taagcggaca tcgatcagcg tcgtcaattt atgagtacac gccctagcac
     1801 atcacaagca tcgataccgg gtctcggcgc gagatcgcat cccggcacaa gagcgatagc
     1861 gatagtgaat tcgtgtctgg actaggtccg ccagtatcac cggcaccggt gctgcggcgt
     1921 cggagccgcg cgacatcaag ctgtttccta cagcgcgcga gatcagcgcc cgtcttgaac
     1981 ccgtagctgc ggcaccggct atcgtcggcg gctcgctgcg caacctcatg ccgttgttct
     2041 gtttgccggg taagggcgca ccctgtcacg gcgagcccga tcatgacggc tgcaacgatc
     2101 cttgatgtta cgcccatcgc cttctctcct ggtgcgatga tggcgagatc gcgcggccgt
     2161 gcgattttag agcttgggtg actcataagc ccggggcggc atccagcgcg acctcggccg
     2221 cgatgtaggc gttgcgcgcg aggacggcaa ggagccgcag ggcagagcga gcttgcccag
     2281 gacctgccgg gcgctagcgc gacatgcggt accgaggacg ccccgagggt gtaggatggt
     2341 caggtctccc gccgcctccg gggcggccat aaccgggaat gggttgcccc gatgctgccg
     2401 ccaaagccgc ccaagccgcc gccgccgagg ccaccgaagc cccctccgcc gccacctgcc
     2461 gcatagcgcg cacgccgtcg cgtggctacg cggcgacgcg cgaggcggcg ggccttcgct
     2521 tagaggtggc ggcgcgaaac gagaggcgca aggaaggcaa ttacggaggc agcctacttt
     2581 gacaagcgct tcaatgctga gtcgcacagc cgctcgggct cgcgttcgcc tcctttcgcg
     2641 agttcgatga tcagcttggc gaacgtcacc gttgtcggat cgctgcggtc cactaatccg
     2701 agctttccca cggcagcctc gaacgccgtc gtcaaagccg cgacgtcttc tggcccgaac
     2761 cagtgattgg tttcgagggc aagactattg ggcatgaaag cccccgcgcc aggctaaagc
     2821 gcgaccggcc tctcggtcac cgcagcctga tgatggaact ccttgtacat ttccggtgga
     2881 caccgccacg acaaagtccg tcttgcccga aatagaaagg cccgccggtg cgcgccgggc
     2941 cgaggtgacc agataaggaa cgccccaaac aaggggcaac attcgatacc ccggccagcg
     3001 ccgcgcgcct agccgccgag cactcccggc tcgacttgta cggctgagcc ggtcgaactt
     3061 tcgagcgcga agacgaggcc gccaactgag gcggcctact ccgcaagctc gcgcaatatc
     3121 ctctctcggt cagcgatgtg catggcctgg agttcttcga attgggcgag cgcggctcta
     3181 taggcgcgta ccggcgattg gcgcgagcgc gatctccatt tcgcggccct cggaaagata
     3241 tttgataagc ggaatattcg cgcgatggag caaccatccc acgaagccga tcctatatgt
     3301 actaggcgct gtacgacggg ggtgggcacg acgggggagc tgcacgcggg agagctgcac
     3361 gccaatctcc cgaatctcgg atgacaggcc gccgaccgcg ccgtgctcga aagggctggc
     3421 agccgcgagc ctgctaatcg tggcctcgct cacaccgtag gagcgcgcca gatcggcctg
     3481 cacagcgccg tcagcgagcc gctccctcac cgccgcgcaa gcagagttct tgacccgtcc
     3541 ggccagcggc ccgagcgata ggacgataag tcgaatagct tctacgatgc cttggaggcc
     3601 aacctcacag acacgctaag cgcctgttta gcggccacta ggagattgag cggttgccac
     3661 ggcttgggca taaaatcgac gccgagcggc agctggccct caggcggatg tccggacgtg
     3721 agaatgaccg gaagaaacgg cgagcgcatc ctgacctcgc gcgccaagtc gatcccatcc
     3781 ataacgccgg gaagccggat atccgcaaag atcatggcga cgtcttgccc gcggattagc
     3841 atttccgtgg caagggcagc ttcggcgctt tcacactcga tggtatcgag ttcgccgtct
     3901 tcaagcagcc tcgccgcgaa ctcgcgcagt tcggcgtcgt cctcgacaat caacaccgct
     3961 cgacgattct gttgggcccc catattcacg cccctcccct gagtgttcgt tacgatctcc
     4021 atccaattgt cgcatgagcg ccgccaggta ttccggcacc ggggcgcaag cggcatcggc
     4081 gaattcggcc cgcagtccgg ccgcaaacgc ctgaaggaac ttcatcgggt cgagggagat
     4141 gaccggcaga cggcgcatca caatgctccg acggacctcc cctggcccaa ccgttcggtc
     4201 gcgcgcctta tgttgtgaat acgacagagg tgcttttgtt ccgacgcggc ggcaagattt
     4261 ttgcgggcat agagctgagc cggtggttcc cggaagcaga cgacgaactg ggccataagc
     4321 agaggggcgc agacgtcctg cgccctttcc cttcttgcgg ccccacgcca aagggcgctg
     4381 gtgactaccc tgtcccaagg cgctctttca acgcgcgctt tagctaaaga aaattccagt
     4441 tccaagggac tggatgctag caccttgatt atctggcgga tcgcgcgcag
//
LOCUS       DATLJT010000212         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_470184,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000212 DATLJT010000000
VERSION     DATLJT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470184"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..499
                     /locus_tag="VM910_04795"
     CDS             170..499
                     /locus_tag="VM910_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61896.1"
                     /translation="MQDGGMNMVSWLCAAGAVILWSSFATLVSHAPDVPPLLLTGVAP
                     ASGSVICLRGADCVAKMAGAMVSFGGCIDAIGSVSGGLAFGLAHAVVHAMASVVRLAE
                     MAKSRGR"
     gene            complement(586..819)
                     /locus_tag="VM910_04800"
                     /pseudo
     CDS             complement(586..819)
                     /locus_tag="VM910_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607949.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1172..>2363
                     /locus_tag="VM910_04805"
     CDS             1172..>2363
                     /locus_tag="VM910_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI61897.1"
                     /translation="MDNSLQILWDDGERVLCRVRRPSECGSHNVLLARPAAERPLPAS
                     LDRLIHDFGLKDELDGAWAVRPLELVREGGRTLLVLEDPGGEPLARLLGAPMEMDDFL
                     RLAVGIAAALGKLHQRGLVHKDLKPAHILVHCVDGQVRLTGFGLASRLPRERQAPEPP
                     ETIAGTLAYMAPEQTGRMNRSIDSRSDLYAFGVTLYQMLTGALPFTAADPMEWVHCHI
                     ARMPVSPRGLVEAVPVEVSDIVMKLLAKAAEERYQTAAGVEHDLQHCLADWRRRHRVE
                     AFPLGEHDTPDRLLIPEKLYGREREVATLVAAFDRIVRSGSPELVLVSGYSGIGKSSV
                     VNELHKVLVPPRGLFASGKFDQYKRDIPYSTLVQAFESLVRPLLGKPETELASWRDAL
                     LEALE"
BASE COUNT          406 a          745 c          770 g          442 t
ORIGIN      
        1 gagagcgaac atgcgccgca ccgtaggtcc ggtttcgggg tagaccggac ttgacgcaag
       61 gcgtgcctta attccgctat gacccggagc ggtctatcga tcgcagaaaa tcgagtggcc
      121 ggtatgcgaa ctcaaccggc cgctcggccc agatcagaac cggttcccca tgcaggatgg
      181 cggcatgaat atggtttcgt ggctgtgcgc ggccggcgcg gttatcctct ggtcgtcgtt
      241 cgcaacgctg gtctctcacg cgccggacgt gcccccgcta ttgctgacgg gcgtggcgcc
      301 ggcgtcgggg agtgtgattt gtctgcgcgg cgcggattgc gtcgcgaaga tggccggcgc
      361 gatggtcagc ttcggcggct gcattgacgc aatcggttcc gtctcgggtg gcttggcgtt
      421 cggtctggcg catgccgtcg tgcatgcgat ggcgagcgtt gtcaggttgg cggagatggc
      481 caagtcacgg ggtagataat aggcatccac aacgagcgtt gacgactgca tggtccacgg
      541 tcccagacat gccacgcaat taaggccgcg tcgtccagac ccaggcaggc ggcagaacca
      601 tcgatacgca acgttaagat gtgcttcttc gcatgacctg cgcccggacc ggacaccgaa
      661 gcaatagccc acgatcaaca cgcggatcat gagccctgga tcaatcgatg gccatcccat
      721 cgggctgtag aacggcgcgg gatgctgacg tcgatcacga aggtcgagga agcaagcgac
      781 gcctctcaac agatgctcgc aagggacgtg ttcgtcgatg tcaaaagagt tccgatgccc
      841 ggttgaccca tcttttcatc agactccacg cggggtatct tcttcgccga aacggcctca
      901 gtggcgcctg gcccgatgcc ggatgcagtc gacgtagttt gaacgcccct cccatcgcgt
      961 ctctccggcc acgccaatgg cgccgcggtc gccgacgtgt gtcctgacga acccgcttct
     1021 gctgcggtgg taatcgcgga gatgtctggt ttaaatcgct ttcgcagcgc atcctgcccg
     1081 aactacgcgg acgaatgtag cgtttaatgg aatgccgggc tgcggcaata gcctgcggcc
     1141 gatgaagaac tgcatagggc tcccggtgcc aatggataac agcctccaga tcctgtggga
     1201 cgatggcgag cgcgtcctct gtcgagtgcg gcgcccgagc gaatgcggca gccacaacgt
     1261 cctgctcgcg cgtcccgccg ccgaacgccc attacccgcc agccttgatc gcctcattca
     1321 cgactttggc ctgaaggacg aactcgatgg cgcgtgggcg gtgcggccgc tggaactggt
     1381 gcgcgaaggc ggccggaccc tgctggtgct cgaggaccct ggcggtgagc cgctcgcgcg
     1441 tctgctcggc gcgccgatgg agatggacga cttcttgcgg ctcgctgtcg gcatcgctgc
     1501 ggcgctaggc aagcttcacc agcgcggcct cgtccataag gatctcaagc ccgcgcatat
     1561 cctcgtacat tgcgtcgacg gacaggtccg gctcactgga ttcggcctcg cctcgcgtct
     1621 gccgcgcgag cgacaggcgc ccgaaccgcc cgagaccatc gccggcacgc tcgcctatat
     1681 ggcacccgag cagaccggcc ggatgaatcg ctcgatcgat tcgcgcagcg acctctatgc
     1741 gttcggcgtc acgctttacc agatgctgac cggcgcgttg ccgtttacgg ctgctgaccc
     1801 catggaatgg gtgcactgcc atatcgccag aatgccggtg tcgccgcgcg ggctggtgga
     1861 ggccgtccct gtcgaggtct ccgacatcgt catgaagctg ctcgccaagg cggctgaaga
     1921 gcgctaccag acggccgccg gtgttgagca tgatctgcag cattgcctcg ctgactggcg
     1981 gcgtcggcac cgcgtcgagg ccttcccact gggcgaacac gacacgccgg accggttgct
     2041 gatacccgag aagctgtatg ggcgggagcg cgaggtcgcc accctggtcg ccgcgttcga
     2101 tcggatcgtt aggagcggct cgcctgagct ggtgctggtc tccggctatt ccggcatcgg
     2161 caaatcctcg gtcgtcaatg aactgcacaa ggtgctggtg cccccgcgcg ggctctttgc
     2221 gtccggcaag ttcgaccagt acaagcgcga catcccctat tcgacgctgg tgcaggcgtt
     2281 tgagagcctg gtgcgcccgc tgctcggcaa gcccgaaacg gagctggcga gctggcgcga
     2341 cgccttgctg gaagcgctgg agc
//
LOCUS       DATLJT010000213         3275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2768033,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000213 DATLJT010000000
VERSION     DATLJT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768033"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VM910_04810"
     CDS             <1..183
                     /locus_tag="VM910_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006589116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HVI61898.1"
                     /translation="AQELRRLLASKGCTFETHKGGSGHVTVRRGDRKSQLPMHGSRKE
                     LGKGLVNKILKDLGLK"
     gene            180..611
                     /locus_tag="VM910_04815"
     CDS             180..611
                     /locus_tag="VM910_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HVI61899.1"
                     /translation="MTLRYPVKLEADDNGTVLATCPSLPEVTTFGEDDADALVHAVDA
                     IEEALAARIAGGEDIPPGPERPRGRSVALSLMTSLKASLYRALRESGITRAELMRRLG
                     WNRESVDRLFRLDHASRLDQIEAAFKALGKSVVLEVEEAGR"
     gene            complement(793..1317)
                     /locus_tag="VM910_04820"
     CDS             complement(793..1317)
                     /locus_tag="VM910_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61900.1"
                     /translation="MPLVAGPQVIAAGESTDRGRAKGIDKSMLTVPTPRRYRSREHLR
                     YVMQQPCLVCGRKPSDPHHLRYMQPRALGRKASDEFAVPLCRGHHREMHRARDERAWW
                     KQAGIDPIKVARRLWKETHAMGDPRSRREAVPRPNGAPVSSNPKNDEISATATTQDEI
                     RRGEMLENRGDVKM"
     gene            complement(1290..2093)
                     /locus_tag="VM910_04825"
     CDS             complement(1290..2093)
                     /locus_tag="VM910_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HVI61901.1"
                     /translation="MPRSSESVAALASALAKAQAELINPEKSLTATIRTGRPGDGEHG
                     FRYAPLSSGLDIVRKTLGQHEIATLQITAIDQTAGMVNLTTTLAHASGEWIASDWPVC
                     PIAETANPQRMGAALTYARRYALFTLVGIAGEDDLDAPDLCAGPNAPSSTAPERALQR
                     GDGLLASQEGPVMSTGAATGKASPVSSSIRLNRRSCGKNCSSRWETSCQPISPSAGQV
                     RRLWRRIRSPQPMRGSWRTLLNGDYRSCHHPMRLHPRSKGLCLWLPGRR"
     gene            complement(2077..2913)
                     /locus_tag="VM910_04830"
     CDS             complement(2077..2913)
                     /locus_tag="VM910_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HVI61902.1"
                     /translation="MATNTLEIRNFAERRAFIGGSDARIIMGSDEAALIRLWQEKRGE
                     AEPEDLSGNLIVQLGAATEALNRTWYERNTGRRITDVQRRVKHSAIPWMVATLDGIVE
                     GTEAVFEAKFMLPWSFSEEAAAEKYMAQLQHNMWVTHLRTSVLSIITGGGKWVEITIP
                     MDPLYLSVLVSAEKKFWRCVQSGEIPHLINAEPSRPRIEAVRIVDMSSSNSWAEFAAL
                     FRNSRHAFLDHERAKSELKALMPEDAKEAIGHGVKAKRSKSGAVSFDVLETEEASHAA
                     VQ"
     gene            3082..>3275
                     /locus_tag="VM910_04835"
     CDS             3082..>3275
                     /locus_tag="VM910_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI61903.1"
                     /translation="MVTTRQVKAARALLGWSQADLAQHSGVSEPTVARLESMDGKLGG
                     REDTAEKIRTAIEIAGVEFI"
BASE COUNT          579 a          994 c         1053 g          649 t
ORIGIN      
        1 gcacaggagc tcaggcggct gctggcctcc aagggctgca ccttcgagac ccacaagggc
       61 ggcagcggcc atgtgacggt tcgccgcggg gaccgtaaga gccagctccc gatgcacggc
      121 agccgcaagg aattgggcaa ggggttggtt aacaaaatcc tcaaggactt gggactgaaa
      181 tgacgctgcg ctatccggtc aagctcgagg cggacgacaa cggcaccgtg cttgccacct
      241 gcccgagctt gccggaggtg accaccttcg gcgaagatga cgccgatgcc ctcgtgcatg
      301 ccgtcgacgc gatcgaggag gcgttggcgg cacgcattgc cggcggcgag gacatcccgc
      361 cgggacccga acgaccgcgc ggccggtcgg tggcgctgtc gctgatgacg tcgctcaagg
      421 cctcgctcta tcgcgcgctg cgtgagagcg gtatcacccg cgccgagttg atgcgtcgtc
      481 tcggctggaa tcgcgaatcc gtcgatcgtc tgttccggct cgatcacgcc tcgcgcctcg
      541 accagatcga ggcggccttc aaggcgctcg gcaaatcggt ggtgctggaa gtcgaggaag
      601 ccgggcgctg agcaggcgct gcacgaacgc ttgcgacaca tcaagctggg cgcggtccca
      661 atggtgtcgg caccatcatc cggcattgcg taaatcggaa ctgccgagtc cgtcacgcca
      721 agcgcggaag catcataggc gttcgctctc cagatcgata tcgggaaaga cattgtgtcc
      781 tcgacgggtg cgttacatct tcacatcgcc acgattctcg agcatttctc ctctgcgaat
      841 ttcatcctgc gtcgttgcgg tggcgctaat ttcgtcgttt tttgggttgg aggagacggg
      901 agcgccgttt ggccgaggta ctgcttcgcg tcgagatcgc gggtctccca tcgcgtgcgt
      961 ctccttccag agcctgcggg caaccttgat cgggtcaatg ccggcttgct tccaccacgc
     1021 gcgctcgtcg cgtgcgcggt gcatttcccg atggtgcccg cggcagaggg ggaccgcgaa
     1081 ttcgtcgctc gctttgcggc ccagcgcgcg tggctgcata tagcgcaaat gatggggatc
     1141 ggatggcttg cggccgcaga cgaggcacgg ctgttgcatg acatagcgaa ggtgctcgcg
     1201 gctgcgatag cgccgcgggg taggaaccgt gagcatgctc ttgtcgatgc ccttggcccg
     1261 accccggtcg gtgctttcgc cggcggcaat cacctgcggc ccggcaacca gaggcaaaga
     1321 cccttcgatc gaggatgcag ccgcatcgga tgatggcagc tccgatagtc tccgttcaaa
     1381 agcgtcctcc acgagcctcg catcggctgc ggtgagcgta ttcttcgcca caagcgcctc
     1441 acctgcccag ctgatggcga gatcggctga cacgatgttt cccacctcga tgagcagttt
     1501 ttcccgcagc tccgccgatt gagccggatc gaggatgaca cggggctcgc ttttcctgtt
     1561 gccgcgcccg tgctcattac cgggccttcc tggctggcca ggaggccatc accgcgctgc
     1621 aacgcacgct cgggcgctgt cgaggacggc gcgtttggtc ccgcgcacaa gtccggcgcg
     1681 tcgagatcat cctcgccggc gatgccgacg agcgtgaaca gggcgtagcg gcgagcgtag
     1741 gtcagcgcag cacccatgcg ctggggattg gcggtctcgg cgatcggaca gaccggccag
     1801 tccgacgcga tccactcacc ggaggcgtgg gcgagcgtgg tggtcaaatt gaccatgccg
     1861 gcggtctggt cgatggcggt tatctgtaag gtggcaatct cgtgttgccc cagcgtcttg
     1921 cgcacgatgt cgagcccgct cgagagcggc gcatagcgga agccgtgctc gccatcccct
     1981 ggccggccgg tgcggatggt ggcggtgagc gacttttccg gattgatgag ttcggcctgg
     2041 gctttggcca aggccgaggc cagggccgcg accgattcac tggaccgcgg catggctcgc
     2101 ctcctccgtc tccagcacgt caaagctcac cgcccccgat ttcgaacgct tggctttcac
     2161 gccatgcccg atggcctcct tggcatcctc gggcatcaaa gctttgagtt cgcttttggc
     2221 ccgttcgtgg tcgagaaagg catgccggct gtttcggaag agcgcggcga actccgccca
     2281 ggaattcgac gaactcatat cgacgatgcg gacggcctca atgcgcggcc gcgacggctc
     2341 ggcgttgatg agatgtggga tttcgccgga ttgcacgcag cgccagaact tcttttccgc
     2401 cgagaccagg acgctgagat agagcggatc catggggatc gtgatctcga cccacttgcc
     2461 gccgccggtg atgatcgaca gcaccgaggt tcttaagtgg gtgacccaca tgttgtgctg
     2521 gagctgggcc atgtactttt ccgccgccgc ctcctccgag aacgaccacg gcagcatgaa
     2581 tttggcctcg aatacggctt ccgtgccctc cacgatgccg tcgagggtcg ccaccatcca
     2641 gggaatggct gaatgcttga cgcggcgctg aacatcggtg atgcgtcggc cggtgttgcg
     2701 ctcgtaccaa gttcggttga gcgcttccgt ggcggcccca agctgcacga tgaggttgcc
     2761 ggacaggtcc tccggctcgg cctcgccccg tttctcctgc cagaggcgga taagggccgc
     2821 ttcgtcgctg cccatgatga tccgggcgtc ggagccgccg atgaaggcgc ggcgctctgc
     2881 aaagttgcga atctccagtg tattcgttgc cattagggta ggtcctttca tggaaatttg
     2941 cgtacgtttg ggctatgggc atccctctag aaatgaccgt tgggctcgct ccgacttggc
     3001 cacggagctg aattgatagg aattctatta actccaggga gcataggagt caatagaaat
     3061 tctatcaaat cgagatgcaa attggtcact acacgtcaag tcaaagcagc acgcgctctg
     3121 cttggctggt cccaagctga tttagcgcag cattccggcg tttcagagcc aactgtggcg
     3181 cggcttgaat caatggatgg caaattgggt gggcgcgaag acaccgcaga aaagattaga
     3241 actgccatcg aaatcgccgg cgtcgagttc attga
//
LOCUS       DATLJT010000214         5030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2537964,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000214 DATLJT010000000
VERSION     DATLJT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537964"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VM910_04840"
     CDS             complement(<1..894)
                     /locus_tag="VM910_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVI61904.1"
                     /translation="MTVRAGREFLAIPGPTTMPDEVLRAMHRPALDIYSREMIDLSDG
                     LHRDLSRLFATKGKSYIHIANGHGAWEAVLSNVLSRGDKVLVLESGRFALNWGLAARA
                     MGAEVEVLEGDWRRAVRPEQVEARLKKDESNEIKAILVAQVDTASGVVNDIEAIGKAV
                     KASGHPALFMVDAVASLGCMPFEMDEWGIDVAMSGSQKGLMTPPGLGFVAANDRAQAV
                     HKTANMRTPYWDWTEREGTEHYRRYAGTAPVHLLFALRQAIDMLYAEGLENVFERHRL
                     LGEAVRRAVAVWAEGQVLSLNI"
     gene            complement(967..1743)
                     /locus_tag="VM910_04845"
     CDS             complement(967..1743)
                     /locus_tag="VM910_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HVI61905.1"
                     /translation="MLLTLLAVLLGYVSLAYIVLPTFWRHYEHQRPLATLSMVTRTAQ
                     GIPGDPINVGLIGDTKEVLCAMNFAGWFPADPVTFRSSIEIAGSVLLDRAYPYAPVSN
                     LFYLGRREDLAFEKPSGKSPDRRHHVRFWKVLDRGQENRPVWLGAVTFDRGVGVSHFT
                     GAITHHIDADIDAEREKLATDLENAGMVTAKYQVTGVGPTLTGRNGGGDLYYTDGEVW
                     IMRLVEDCRKQDGNVTIMPSPPATELKDQIWRSITQTLGR"
     gene            complement(1826..2551)
                     /locus_tag="VM910_04850"
     CDS             complement(1826..2551)
                     /locus_tag="VM910_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVI61906.1"
                     /translation="MSMKFLFAALGLLLPSLASAADITGAAKIREADTIQIGNSRIRL
                     AGIDAPSVDQLCLNNKGDRWTCGVAARDELIRHTEGRNWTCHVGQTDRRGRSLARCEV
                     DGEDIQRWLVRSGWALAYTRFSRDYEADEAQAREAKVGMWQGAFIAPWDWRVRNKKTP
                     ILGAANAPENARAVLLASASGEVAPSPDCTIKGNVNSAGECIYHRPTSRWYAQIKMRI
                     SKGTRWFCSVEEAEAAGCRETRR"
     gene            complement(2719..4452)
                     /locus_tag="VM910_04855"
     CDS             complement(2719..4452)
                     /locus_tag="VM910_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61907.1"
                     /translation="MSFYRTAGAACRAMTARKDGLSLYDLCDPLLLNAKSGDAHLSKF
                     YRTAIGNPALRALLRRTGLPELVDGQFVSLREALIHARDAASPDWERVGAPVAALLDG
                     IKLSHPSPKPAKPNGAAPKPSEIDQLIKTCATHLLGSFRKNGFIPAYAAFNLIGDADV
                     KGRELLMALTGLNSRGYKNSTLLFNLARIFIARSEARILINPPWTGIAEPMWEPMQIR
                     HRSAYYDAFFTEALLAYTESGLASAAETAAARGAIAEMVEFCLSTSREEVRGRDGKAY
                     NVITALAPLPHPRFSRFFAQIKQDLGFGIYVPDCDTTACSFSAATQAGSDDPILEQPL
                     LDFYAGYQARAGDNEPRVTVPLNDNIDFEGGVATWIDNLRGDRPFGNDLDPTLNLDIL
                     EVSFRNLARWKVLETRERLETVQRIVAFQRQLAESGAFADPRSHIYYLPELYCAYFGR
                     CYAAFMALPAAARQAIDPAGSFGLIREKVLVYVEGELITYEMNPFDAALALIALGHLG
                     ADAAAFAPALNCIAHHLGEGGRHGPFLAYEWNKMKTPTRILVGGPEVTSAFVLMGVAL
                     ARRAMRGRGRA"
BASE COUNT          862 a         1579 c         1662 g          927 t
ORIGIN      
        1 gatgttgagc gacagcacct ggccctcggc ccacacggcg actgcgcgcc gcaccgcttc
       61 gccgagcagg cggtggcgct cgaacacgtt ctcgagccct tcggcgtaaa gcatgtcgat
      121 cgcctgtcgc agcgcgaaca gaagatgcac cggcgcggtg ccggcatagc ggcgatagtg
      181 ctcggtccct tcgcgctcgg tccaatccca atagggcgtg cgcatgttgg ccgtcttgtg
      241 cacggcctgt gcgcgatcat tggccgcgac aaagccgagg cccggcggcg tcatcaggcc
      301 cttctgcgaa ccggacatcg cgacatcgat gccccattcg tccatctcga acggcatgca
      361 accgagggaa gcgaccgcat cgaccatgaa gagggcggga tggccggagg cttttacggc
      421 cttgccgatc gcttcgatgt cgttgacgac gccggaggcg gtgtcgacct gcgcgaccaa
      481 aatcgccttg atttcgttgc tctcgtcttt tttcagccgc gcctcgacct gctcgggccg
      541 cactgcgcgc cgccagtcgc cctcgagcac ctcgacctcg gcgcccatcg cgcgcgcggc
      601 aagaccccag ttgagggcga agcggccgct ctccagaacc agcaccttgt cgccgcgcga
      661 cagcacgttg ctgagcaccg cttcccaggc gccgtggccg ttcgcgatat ggatgtagga
      721 tttgcccttg gtcgcgaaca gtcgcgagag atcacggtgc aggccgtcgc tcagatcgat
      781 catctcgcga gagtagatgt ccagcgccgg ccgatgcatc gcccgcaaca cctcgtcggg
      841 catggtggtt ggcccgggga tggccagaaa ttcccggccc gcgcgaacgg tcattgttga
      901 gattccttgg ttcctgacgg cccatggatg cagtctatcg gggcgaaatt caagctcagg
      961 gcgggcttat ctgccaagcg tctgcgtgat gctgcgccag atctggtcct tcagctcggt
     1021 cgccggcggg ctcggcatga tcgtcacatt gccgtcctgt ttgcggcaat cttcgaccag
     1081 ccgcatgatc cagacttcgc cgtcggtgta atagagatcg ccgccgccgt tgcggccggt
     1141 gagcgtcggc ccgacgcccg tcacctggta tttcgcagtc accatgccgg cattttccag
     1201 gtcggtcgca agcttttcgc gttcggcatc gatgtcggcg tcgatgtggt gggtgatggc
     1261 gccggtgaaa tggctcacgc cgacgcccct gtcgaaggtg acggcgccga gccacaccgg
     1321 gcgattctcc tgaccgcgat cgagcacctt ccagaatcgc acatggtggc ggcgatcggg
     1381 actctttccg gacggctttt cgaaggcaag gtcttcgcgc cgcccgagat agaagaggtt
     1441 gctcacgggc gcatagggat aggcgcgatc gagcagcacg cttcctgcga tctctatcga
     1501 ggatcgaaaa gtgacggggt cggcgggaaa ccagccggcg aaattcatcg cgcacaagac
     1561 ctctttggtg tcgccgatca gcccgacatt gatgggatcg ccgggaatgc cttgcgcggt
     1621 gcgcgtcacc atcgacagcg tggcgagcgg tctctggtgc tcgtaatgtc tccagaacgt
     1681 cggcagcacg atgtaggcga gcgagacata gccgagaagg accgcgagca gcgtcagcag
     1741 cacgagctcg agccgggcgt gatgatgggc ggcaacatgc gcatcatgat gctcgtcgct
     1801 cacggtcgag ccgtccttgc cgtggctacc gcctcgtctc gcggcagccg gcggcttcgg
     1861 cctcctccac cgagcagaac cagcgcgtgc ccttggagat tctcatcttg atctgagcat
     1921 accagcggct ggtcggcctg tgatagatgc actcgcctgc gctgttgacg ttgcccttga
     1981 tggtgcagtc gggcgagggg gcgacctcgc cggaagccga ggcaagcagc acggcgcggg
     2041 cgttttcggg tgcgttggcc gcgccgagga tcggcgtctt cttgttgcgc acgcgccagt
     2101 cccacggcgc gatgaaggcg ccctgccaca tgccgacctt cgcctcgcgg gcttgcgcct
     2161 cgtcggcttc gtagtcgcgg gagaagcgcg tgtaggcgag tgcccagccg cttcgcacca
     2221 gccatctctg gatatcctcg ccgtcgacct cgcagcgcgc gagcgaccgg ccgcgccggt
     2281 cggtctggcc gacgtggcag gtccagttcc tgccttcggt gtgcctgatg agctcgtcgc
     2341 gggcggcgac cccgcaggtc cagcggtcgc ccttgttgtt gaggcagagc tgatcgaccg
     2401 atggcgcgtc gatgccggcg aggcggatgc ggctgttgcc gatctggatt gtgtcggcct
     2461 cgcggatttt ggcggcgccg gtgatatcgg cggccgaagc gagcgacggc agcagcaaac
     2521 cgagcgcggc aaacaagaat ttcatggaca tcaacggccg ccgagccttg gggaatcgcg
     2581 ccttggagaa tcgagccttg gagaatcgcg cgatagtcgc gccaattgtg gtgcggattt
     2641 ggccggagcg ccagcggctt tgctgacagt tcccgcctac ggcaacctta ccctcccttc
     2701 atgcggaggg cggcatcgtc aagcccttcc tcgtccccgc atcgctcttc gcgccagcgc
     2761 aacgcccatc agcacgaaag cggaggtgac ttcgggaccg ccaacgagaa tgcgcgtcgg
     2821 cgtcttcatc ttgttccatt cataggcgag aaacggccca tgccggccgc cttcgccgag
     2881 atggtgcgcg atgcagttga gcgccggcgc gaacgctgcg gcgtcggcgc cgagatgccc
     2941 gagcgcgatc agcgccagcg ccgcgtcgaa cgggttcatc tcgtaggtga tcagttcgcc
     3001 ctcgacatag acgagcacct tttcgcggat cagcccgaag ctgcccgccg gatcgatcgc
     3061 ctgccgcgcg gccgccggca gcgccatgaa ggccgcatag cagcggccga aataggcgca
     3121 gtaaagctcc ggcagataat agatgtgcga acgcggatcg gcgaaggcgc cgctttcggc
     3181 gagctgccgc tggaaggcga cgatgcgctg gacggtctcc agccgctccc gcgtctccag
     3241 taccttccag cgcgcaaggt tgcggaaact gacttcgaga atgtcgagat tgagcgtcgg
     3301 atcgaggtcg ttgccgaacg ggcgatcgcc cctgagattg tcgatccagg tggcgacgcc
     3361 gccctcgaaa tcgatgttgt cgttgagcgg cacggtcacg cgcggctcgt tgtcgccggc
     3421 tctcgcctga tagccggcgt agaagtcgag cagcggctgc tccaggatcg gatcgtcgga
     3481 ccccgcctgc gttgcggcgg agaacgagca ggcggtggtg tcgcagtccg gcacgtagat
     3541 gccgaagccg aggtcctgct tgatctgcgc aaagaagcgg gaaaagcgcg gatgcggcag
     3601 cggcgccagc gccgtgatca cgttgtaggc cttgccgtcg cgcccgcgca cctcttcgcg
     3661 gctggtcgac aggcaaaact ccaccatctc cgcgatcgcg ccgcgcgcag cggcggtttc
     3721 tgcggcggac gcgagcccgc tctcggtata ggcgagcagc gcctcggtga agaacgcatc
     3781 gtaataggcc gagcgatggc ggatctgcat cggctcccac atcggctcgg caatcccggt
     3841 ccagggcggg ttgatcagga ttcgcgcttc cgagcgcgcg atgaagatgc gcgccagatt
     3901 gaacagcagc gtcgagttct tgtagccgcg cgagttgagc ccggtcagcg ccatcaggag
     3961 ctcgcgcccc ttcacgtcgg cgtcgccgat caggttgaag gcggcatagg ccgggatgaa
     4021 gccgttcttc cggaacgaac ccagaagatg cgtggcgcag gtcttgatga gctgatcgat
     4081 ctcggagggc tttggcgcgg cgccgttggg cttggccggc ttcgggcttg gatgcgacag
     4141 cttgatgccg tcgagcagcg cggccacggg cgcgccgacc cgctcccaat cgggcgaggc
     4201 cgcgtcgcgc gcgtggatca atgcctcgcg cagcgagacg aactggccgt cgacaagctc
     4261 gggcaggccg gtgcggcgga gcagggcgcg cagcgccgga ttgccgatcg ccgtgcgata
     4321 gaatttggaa agatgcgcgt cgccgctttt cgcattgagc agcaggggat cgcagaggtc
     4381 gtagagcgag aggccgtctt tgcgggccgt catggcgcgg caggcggcac cggcggttct
     4441 gtaaaagctc attcgcccct ttttctttcc cgcgcgttct taggactatc cgcccccgcc
     4501 cggcggccgc agcgtcgagg ctgctcgttt ttgaacgcta tacgacaaaa catgatccgg
     4561 gaaccggcgg catgcgcatg tcaccctctg gttttccctc ccctgcaaga gggaagtgaa
     4621 ggtggcaagg cgcaaggcga ggtggtcaag cgcgaacgga ggcccggctg atgtcctccc
     4681 gctgtgcgcc tgacgacgca atacaagccc tcccttcgcg agccaaattt cttgtgtgac
     4741 attggtcaca gcgccatgca cggatttgcc accatgatcg cagaaatgat gcttcgatga
     4801 tggcgtcgac ccggttatac gtcaaattcc atagtgatat caatttgttc cgtggaatgc
     4861 actgttaact attgccggaa cctccgtcac gggttaaggg tttgtttgtt gcggtgccgc
     4921 aaattgatcg caatttagac ggcacctccg accggcgaac ccccctcaaa cccggaaggt
     4981 tttggcgcga ataagaaatc gcgtgtttgg cagtgagcag gaaacgtacc
//
LOCUS       DATLJT010000215         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1848699,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000215 DATLJT010000000
VERSION     DATLJT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848699"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..1813)
                     /locus_tag="VM910_04860"
     CDS             complement(437..1813)
                     /locus_tag="VM910_04860"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HVI61908.1"
                     /translation="MGGDNRFRIGFFGDVRREQAGATLFERVVETGSLVLRQVGGDRA
                     GEMAAHRFLDSPHVTPQEILNTAAGRTAAACCGRRIVAAQDTTEINFSGRSRGRKGLG
                     PAGDGETPGFFCHAMVAVDVESEAVLGVVHAHIWTRSTEEMVARRSRTIEEKESFRWI
                     QGSTVAGTLLCAAAQLIVVGDRESDIYSQFARVPMGAELIVRAAQNRKLDNDEELFDA
                     ACNWRELGTMEIAVPPHRPGEAARGACVGVKAGAVRVVKPRNGADPGDPATVALTYVE
                     VCEINAPKGHTPIIWRLLTTLPVCGAADEFAAAMDIVRLYRLRWRIEQVFRSMKSDGL
                     RLEETQVQEASRLFKLAAIALTAAVRTIQLVDARDGSPRPASDVADEDLIRAAGAIGP
                     TLERKTARQQNPHPSGSLAWLSWIAARLGGWNCYYKPPGPKTMRAGWNKLEAMAAGYA
                     IASARQNP"
BASE COUNT          449 a          698 c          619 g          424 t
ORIGIN      
        1 tcctcgcggc gcaaacggcc actcggatcg ttgttcgcaa cgtcctgagg cctcgacgtc
       61 gccacacttt gcgttgattg ctcaatctgg agcattaccc gcgtgcgccc cttcagaagg
      121 ctggaccatg tcgttccttc cgcggcttct cgcgcccgtt atcatgatga gcatgatgtg
      181 tggaacggca ctgccgcaga cggcccggcc ggagggcata ccaagtccgg atgatacgtg
      241 gacgctgcca aatcgcgatg ccatgaatgc gaatactgtc accgtcatca cggcaccggc
      301 gggtggtgcc accgctatct tcggctccga tatggcgcgg gtactcgaca atggcgagct
      361 ccgcgtgctt ccggtcctcg gcaaggggcc agttcgcaac gtcgtcgaca tcctctacct
      421 caaggcccta cgggattcac ggattttgtc tagcgcttgc aatagcataa ccggctgcca
      481 tggcttcgag tttgttccaa ccggctcgca tggtctttgg gcctggtggt ttgtaatagc
      541 aattccatcc gccaaggcga gcggcgatcc aggagagcca agcgagagaa ccggacggat
      601 gcgggttttg ttggcgcgcc gttttgcgct caagcgtcgg gccaatggct ccggccgcac
      661 gaatgagatc ttcatcggcg acgtccgacg ccggacgagg acttccgtcc ctggcatcaa
      721 caagttgaat ggtacgcacc gcggcggtca gcgcaatggc cgccagcttg aacaggcgcg
      781 aggcttcctg gacttgggtt tcttccagcc gcagtccatc agatttcatc gaccggaaga
      841 cctgttcaat tctccacctc agccggtaaa gacgcacgat gtccatggcc gcggcgaatt
      901 cgtcagcggc tccacaaacc ggcaatgtgg tcagcagtcg ccagatgatc ggcgtatggc
      961 ctttaggtgc attgatctcg cagacttcaa cataagtgag cgccacggtt gctggatcgc
     1021 cgggatctgc tccgttgcgc ggcttgacga cgcggactgc accggccttc acgcccacac
     1081 aggcgccgcg cgcggcttcg ccgggtcgat gcggcggaac agcaatttcc atggtgccga
     1141 gctcgcgcca gttgcacgca gcatcgaaca gctcctcatc attgtcgagc ttgcgatttt
     1201 gcgcggctcg tacgatcaac tctgccccca ttggaacacg ggcgaactgc gaatagatgt
     1261 cgctctcccg atcgccgacg acgatcaatt gcgcggctgc gcaaagcaac gtgcccgcaa
     1321 ccgtcgagcc ctggatccaa cggaaggatt ccttctcctc aatcgttcgc gatcggcgag
     1381 ccaccatttc ctccgtcgat cgcgtccaga tgtgcgcgtg caccacgcca agcactgcct
     1441 cgctttcgac atcaaccgcc accatcgcat ggcagaagaa tcccggcgtc tccccgtcgc
     1501 ctgctggtcc aagacctttg cgcccgcgag atcgtccgct gaaattgatc tcggtcgtat
     1561 cctgggcggc aacgatccgc cgtccgcaac acgccgccgc ggttcggcca gccgcggtgt
     1621 tcaggatttc ctgtggggtg acatgcggcg aatccagaaa gcggtgtgcc gccatctctc
     1681 cggcccgatc acctccgact tgacgtaaaa ccaatgatcc agtctccacc acgcgctcaa
     1741 acaaagttgc tccggcctgt tctcgacgca catcgccgaa aaagcctatt cggaacctat
     1801 tatcgccacc catcccagcc cccgctgtcg tccggacggg cagcgaatca catcgtaaag
     1861 gcctcgggga atcgtaaaat tttaccgaca gactcttgac gaagcgcgca aaaaatgccg
     1921 atcaacgaat caaatctgtg aacgccgtag ctctgccggg gaggcagcaa aagtttgaca
     1981 tatgaagaag cagtccaccg gcgacactct cccatcgtga gctgcccaag cgcacgaacg
     2041 ggaagtgacc cggtggacca agtatcgcag gctaagctgg aacaagcggc tgtgcaaatg
     2101 ccacgcggtc acgcaaaaga cccagtgggc ttggcgatga aggtcgccac gtggattaat
     2161 tggggggagc gcaagcgacc cccaaacagc
//
LOCUS       DATLJT010000216         8666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_240362,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000216 DATLJT010000000
VERSION     DATLJT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8666
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240362"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VM910_04865"
     CDS             <1..316
                     /locus_tag="VM910_04865"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI61909.1"
                     /translation="RKSLDWHGYAEKIRVPYLIAGGESDELCPLENTKAFAKALGGPK
                     QLIIYADARHSIGGVPSATNGPDPRAYQAKWVQARLVGKPLTSEYWFVEPSGRIDKTP
                     LS"
     gene            complement(741..1454)
                     /locus_tag="VM910_04870"
     CDS             complement(741..1454)
                     /locus_tag="VM910_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI61910.1"
                     /translation="MTIGVVLAAAFAISGAAQDVIAPSGAPAVAFPKPDRPVADIVSP
                     IWHDEKERDDAGEPGQLVRLLGIKSGMTVADIGAGSGYYVVRLSPIVGSNGRIIAEDI
                     VPKYLQSLRQRVRNLGLQNVVISLGEPHDPKLPAHSVDIAILVHMYHEIEQPYALLYN
                     LVTALQPEARVGIVDAFGPTSQHGTPPSLLRCELAAAGYREISFDRLTGSNAYLAIFA
                     PPSVASRTRPEAIVACKAR"
     gene            complement(1564..2553)
                     /locus_tag="VM910_04875"
     CDS             complement(1564..2553)
                     /locus_tag="VM910_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI61911.1"
                     /translation="MKLTRRRLRSLTAGAAALPALSRSAGAQVYPSRPVTIIVPVPPG
                     GVADPIARILADHLTVTLGQPVVVENVSGAGGSIGVGRAVRAAPDGYTLSIGNWLSHV
                     GASAVYPVQYDVLKDFEPVSLLTNSPILITARKDFPANDLKELIAWLKANPDKATAAT
                     VGAGSASQVSGVYFQRATDTRFQFVPYRGGGPAVQDEVAGHVDLMFNEGTVALPYVLS
                     HQIKTYAVLAKTRWFAAPDIPTSEELGVPGINISFWHGLWVPKGTPKEIVAKLNAAAV
                     DALAEATVRKRLTDIGQEIFPRDKQTPETLYAFHKAEIDKWWPIIKAAGIKAE"
     gene            3011..3781
                     /locus_tag="VM910_04880"
     CDS             3011..3781
                     /locus_tag="VM910_04880"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI61912.1"
                     /translation="MPACIEGRNVNLTFRPPNRRPVRALHGFDIDVEECEFLSIVGPS
                     GCGKTTFLNVLLGLVKPDSGELRIRGKPISGPGTDRAMVFQEFGLLPWRTVLNNIELG
                     LELKGVPVETRRHISRRFVHMVGLAGFEGHYPHELSGGMKQRVGLARALATDPDVLLM
                     DEPFAALDAQTRDVMQVELLRIWQEARKTVLFVTHQIDEAIYLSDRVMVMTKRPGHAK
                     KIFTIKLPRPRDYEMRVTREFNDLKLEIWQELKDEITV"
     gene            3784..4575
                     /locus_tag="VM910_04885"
     CDS             3784..4575
                     /locus_tag="VM910_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61913.1"
                     /translation="MMDDAAPRVSFPARYRKALLGSLSVVLFFAAWQAVFLVVPFDPL
                     FISKPSLIASGLVDLITSGDLFRDLAVSAVPFLYGFSAAVVIGVPLGVVMGWRVRVGY
                     ALDPLMTVFYASPLVALAPLVVIFFGVGIGGKTLIIFLLSVFPFIFNAYAGVRAVDRL
                     LINVVRSLGGRERDLYFKVILPSILPYIIAGARIAVGRALIGVLVGEFFAASEGIGYA
                     ISRFGDIFALDRMFACIIVIMIIAVVLTEGIRWTERTAFPWRTRQ"
     gene            4580..5440
                     /locus_tag="VM910_04890"
     CDS             4580..5440
                     /locus_tag="VM910_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI61914.1"
                     /translation="MSEIQAVDANVAAVAPVDDLAAPRKGMWQRFEPAILGCGSIAVL
                     LLVWEFLPRLITMQEGTKLFFTVPSEIGNTLWRMFATGAIWAPLGVSASAFAIGLSIA
                     IAVGLPLGVLLGRSAALNAMFDPFITAFNATPRLVFLPLLLLWFGLGLWSKVMIVFIG
                     ALFPILINTYEGVRNADRVLINVVRSFGATEWGIARLVVVPNALPYIVVGLRLAIGRA
                     VLGVVVAEFFGSEDGLGVVMVRAASRFQVDVVFAGLIVFAALSLLMTGLVKLLEDRLS
                     RWRPQHISAT"
     gene            complement(5495..6520)
                     /locus_tag="VM910_04895"
     CDS             complement(5495..6520)
                     /locus_tag="VM910_04895"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61915.1"
                     /translation="MRMLRCLLVRVTAALALCLAIATAHAQTKISIGKVIGGDGFHIP
                     SYVAMDRGFFKSESLDARFVELQGKAQVTAVLSGNLDFAPIPSGGSQAALSGAKIKYI
                     VGESLKSQWTVTVRKEINKPEDLKGKIVGYGRAGGADYDEAEAVLHRFFHMDVGKDYK
                     VISFQGEPERIAALINGDIQGASLSIPHAATAVNVGLKVLLRTGDYIPRAGGTIWGMQ
                     ANVDNNPETVKKVIRAIAKAVMYFRDNKQGSIPILKHHLGIKTDAEAAIIWDQLHDAF
                     GAEVPKDLFRAIFESRRLTMIAARQWPQDQPLPDPEQFLARDLLDSTLKEMGYVPTKL
                     EAPTKTN"
     gene            complement(7412..7657)
                     /locus_tag="VM910_04900"
     CDS             complement(7412..7657)
                     /locus_tag="VM910_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61916.1"
                     /translation="MEALRSLARSSELEANRLPTAELRIRDFLTPVGTNRAAQRAALE
                     ELDNAVSREAELRPREASFWQAISDFIAQRLAMLNFD"
     gene            7802..7948
                     /locus_tag="VM910_04905"
     CDS             7802..7948
                     /locus_tag="VM910_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61917.1"
                     /translation="MASYIRRRKFLATLGGAAAWPLAARAQQRKRWRRLISFERRASD
                     HSCD"
     gene            8306..>8666
                     /locus_tag="VM910_04910"
     CDS             8306..>8666
                     /locus_tag="VM910_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVI61918.1"
                     /translation="MFKSKAPEKDKDVAADTTEVVGRTVQQQQSTVEQPPPRREPTTN
                     AAIRSCIGSGMSIVGNIECNGPAQVFGRIEGEVRASDLLIGDGAQIEGSVIAQDVTVC
                     GRVKGTIRAVRVKLQDSG"
BASE COUNT         1568 a         2603 c         2682 g         1813 t
ORIGIN      
        1 ccgcaaatca ctcgattggc acggatacgc cgaaaagatc agagtgccgt acctgatcgc
       61 agggggcgaa tctgacgagc tctgtcccct ggagaatacg aaggccttcg ccaaagcgct
      121 cggcggcccg aagcagctca tcatctatgc tgacgcccgc cacagcattg gcggggtacc
      181 atctgcaacc aatgggccgg atcctcgcgc ctaccaagcc aaatgggtac aggcgcggct
      241 tgtcggtaaa ccgctcacca gtgagtactg gttcgtcgaa cccagcggtc ggatcgacaa
      301 gacgccactg tcgtgatcag cagacaatcg atcatattgg ctcgctgccc agcgatattc
      361 gatcgtgatg ttctggccat cgacgaaacc caggtcgctt agtccttgtc gaaaggaagc
      421 tgcgaggtaa gcgtacttgt cagctgacgc gttgttgagg aagccgacag ctggcctggt
      481 tacctgctct gccctcgtcc ggagcggcca cgccgccgcg ccgccgagtg cggctagcaa
      541 ttcccgccct ccgatgctga ttatcatgtg cccctcccgc ctgagggtgc gtaaaaggaa
      601 cgatatcacg ccaccagcgt acggtgttcg ccctacttcc ggtggcacac tcgggcgagg
      661 ttctcgtttt tggccaccgg cgggaacgac tacactcgac gaacgcgcgg cgtttcccgc
      721 tcccgtcatg acgcaatccg ctagcgcgcc ttgcacgcga ctatcgcttc cggtcgggtg
      781 cgacttgcaa cggacggtgg cgcaaagatc gcgagatagg cattgctgcc ggtgagccga
      841 tcgaaactga tctcgcgata cccagcggct gcgagctcgc agcgcaggag acttggcggg
      901 gtaccgtgct gggatgtcgg tccgaaagcg tcgacaatgc cgacgcgcgc ttccggctgg
      961 agcgcggtga cgagattgta gagcaaggcg taaggttgtt cgatctcgtg atacatgtgc
     1021 accagaatcg caatgtcgac cgagtgagcc ggcaacttcg gatcgtgcgg ctcaccaagg
     1081 ctgatcacaa cgttctgcaa tccgagattg cgcacccgct ggcgcaggct ttgcaaatac
     1141 ttggggacga tgtcttccgc gatgatgcgg ccgttagatc cgacaatcgg cgacagccgt
     1201 acgacgtagt agccgctacc cgcgccaatg tccgcgactg tcatcccgga tttgatgccg
     1261 agcagacgca cgagttggcc cggttcgcca gcatcgtcgc gctccttttc gtcgtgccag
     1321 atcggactga cgatgtccgc gaccgggcga tcgggtttcg gaaaggcgac cgccggcgcg
     1381 cccgatggcg cgatgacgtc ctgcgcagcg cctgaaatgg cgaatgcagc ggccaggacg
     1441 acaccaattg tcattcgcat cattacggcc tgaactcagg tggcaaattg ccttcgtgcg
     1501 gacggaagcg ccatgggcac ggctagtcgt ggacgatcgc tcgcagacgc gtcatgcggt
     1561 tgcttattca gccttaatgc cggccgcttt gatgatcggc caccatttgt cgatctcggc
     1621 cttgtggaag gcgtaaagcg tttccggagt ctgcttgtcc cgcgggaaaa tctcctggcc
     1681 gatgtcggtg agccgcttgc gtaccgtcgc ctcggcgagc gcgtcgaccg ccgcggcgtt
     1741 gagctttgcc acgatctcct tcggcgtgcc cttggggacc cacagcccgt gccagaatga
     1801 aatgttgatc ccgggcacgc cgagctcctc gctggtcggg atgtccggtg ccgcgaacca
     1861 gcgggtcttg gccaacactg cataggtctt gatttggtga ctgagcacgt aaggcagcgc
     1921 aaccgtcccc tcgttgaaca tcaggtcgac atggccggcg acctcgtcct ggacagcggg
     1981 gccgccgcct cgatagggca cgaattggaa gcgggtgtcg gtcgcgcgtt gaaaatagac
     2041 gccactcact tgtgacgcac tgccggctcc gacggttgct gccgttgcct tgtccggatt
     2101 tgccttgagc caggcgatca gttccttgag gtcgttcgcc ggaaaatctt tccgggcggt
     2161 gatgagtatc gggctgttgg tcagcagcga taccggctcg aaatccttga gcacgtcgta
     2221 ttgcaccggg taaacggcgc tcgcgccgac gtggctgagc caattgccga tactcagcgt
     2281 atagccgtcc ggcgctgcgc gcacggcgcg gccgacgccg atgctgccgc ccgcgccgct
     2341 gacgttctcg accaccacgg gctggccgag cgtgaccgtc agatggtcgg caaggatccg
     2401 cgcgattggg tcggcgaccc cgccaggcgg caccggcacg atgatggtga ctgggcgcga
     2461 cgggtagacc tgcgctcctg cactgcgcga aagggccggg agcgcggcag cgcccgctgt
     2521 cagggaccga agtcgacggc gcgtaagttt caaggtggtc cccgattgga tttgcaagcg
     2581 cagcgtatca cgcgctgcaa acaattcgag agctttgaga gggcgtgtcc gctaagaaca
     2641 gcttacagtc ggtattgggg gtgcctcgga catgaatgga cctgtcgccc cgcccgaccc
     2701 agtcgagctc tacccggacg gcatgcgccg ggtcgtcacc ggcatgagct cgtcggggcg
     2761 ttgttgcgcc ttgcccctga tcacggagtc ttggcggggc aggcgcagaa cttcggagcg
     2821 caaatccgat ccctcctata ccccttcaaa cctgagcctg gaggtaacct atccaaggct
     2881 caacatcggt cgacgctgtg gtatgttttg gcgcggccgg cggcccgaat tgtcgcacca
     2941 tgcgacaatt attcccgcgc gatccgaacg cgaaagcgac actctggaca gcgaggcgga
     3001 gtcaagctgc atgccagcgt gtatcgaagg ccgcaacgtc aatcttacct tccggccgcc
     3061 gaaccgccga ccggtgcgtg cgcttcacgg cttcgatatc gatgtcgagg agtgcgagtt
     3121 cctgtcgatc gtcggtccgt ccggctgcgg caagaccact ttcctcaacg tgttactcgg
     3181 gctcgtcaaa ccggattccg gcgaactacg catccgcggc aagccgatct ccggaccggg
     3241 caccgaccgg gcgatggtct tccaggagtt cgggctcttg ccgtggcgca cggttctcaa
     3301 caacatcgag cttgggctcg aactcaaagg cgtcccggtc gagacgcgtc ggcacatctc
     3361 ccgccgcttc gtacacatgg tcggtcttgc cggattcgaa gggcactatc cgcatgaact
     3421 gtcgggtggc atgaagcagc gtgtcggact tgcgcgcgcg cttgcgaccg accccgacgt
     3481 gctgctgatg gacgagccgt tcgctgcatt ggatgcgcag acgcgtgacg tcatgcaggt
     3541 cgagttgctg cgaatctggc aggaggcgcg caagacggtc ctgtttgtca cccatcagat
     3601 tgacgaggcg atctatctct ccgaccgcgt catggtgatg accaagcggc ccggccatgc
     3661 caaaaaaata ttcacgatca agctaccacg gccgcgcgac tacgagatgc gggtgacgcg
     3721 agaattcaac gatctcaagc ttgagatttg gcaagagctc aaggacgaga ttacggtcta
     3781 ggcatgatgg acgacgccgc gccacgagtg agttttcccg cgcgctatcg aaaggcgctg
     3841 ctcgggagcc tttcggttgt gctgttcttc gctgcttggc aggcggtgtt tctcgtcgtc
     3901 ccgttcgatc cactgttcat cagcaagcca agcctcatcg caagcggtct cgtcgacctg
     3961 ataacgagcg gggatctgtt ccgcgatctc gccgttagcg cggtgccgtt cctctacggt
     4021 ttctcagcgg ctgtagtcat cggcgtcccg cttggcgtcg tcatgggctg gcgcgtgcgc
     4081 gtcggttatg cgctcgatcc gctgatgact gtgttctatg cgagcccgct cgtggcgctt
     4141 gcgcccctgg tcgtgatctt tttcggcgtc ggcatcggcg gcaagacact cattatcttt
     4201 ctcctctcgg tctttccctt catcttcaat gcctatgccg gggttcgcgc cgtcgatcgc
     4261 ttgcttatca atgtagttcg gtcgctgggc ggccgcgagc gagatcttta tttcaaggtc
     4321 atccttccaa gcatactgcc gtacatcatt gccggcgcac gcattgccgt ggggcgcgcg
     4381 ctgatcggcg tgctggttgg cgagttcttc gcggcctcgg aaggaatcgg ttacgccatt
     4441 tcccggttcg gcgatatttt cgcactcgac cgaatgtttg cctgcattat cgtcatcatg
     4501 atcatcgccg tggtgctgac ggaaggcatt cggtggaccg aacgcacggc gttcccatgg
     4561 cgaacccggc agtaggacca tgagcgaaat ccaagccgtt gacgccaacg ttgccgctgt
     4621 cgcgccggtc gacgaccttg ccgcgccgag gaagggcatg tggcaacggt tcgagccggc
     4681 cattctcggc tgtggtagca tcgcggtcct gctgctggtc tgggagttcc tgccgcgtct
     4741 cattacgatg caggagggaa ccaagctgtt cttcacggtg ccgagcgaga tcggcaacac
     4801 cctgtggagg atgtttgcca ccggagcgat ctgggcgccg ctcggcgtca gcgcctcggc
     4861 ctttgcgatc ggactgtcga tcgcgatcgc ggtcggcctg ccgcttggcg tcctgctcgg
     4921 gcggtcggct gcgctcaatg cgatgttcga tcccttcatc accgctttca atgcgacacc
     4981 acggctggtg ttcctgccgc tcctcctact gtggttcggg cttggcctgt ggtcaaaggt
     5041 catgattgta ttcatcggcg ccctgtttcc gatcttgatc aatacctatg aaggggtgcg
     5101 caacgccgac cgagtcctga tcaatgtcgt gcggtcgttc ggggccacgg aatggggcat
     5161 cgcccgcctg gtggttgtcc cgaatgcgct gccctatatc gtcgtcggct tgcgcttggc
     5221 gatcggccgt gccgtcctcg gcgttgtggt ggccgagttc ttcggttccg aagacggtct
     5281 tggcgtggtc atggtgcggg ccgcgagccg ctttcaggtg gacgtcgttt ttgccgggtt
     5341 gatcgtgttc gccgcgctgt ctttgctgat gaccggactg gtaaagttgc tcgaggatcg
     5401 gctgagccgc tggcgtccgc aacacatcag tgcgacttga ttggactggg cttcgtccct
     5461 ccgcgaaagc taaaccaggc tcgggctaga tcggctagtt cgtctttgtc ggcgcttcaa
     5521 gtttggttgg aacgtagccc atttccttca gcgtcgaatc gagcagatcg cgggccagga
     5581 actgctccgg gtccggcagc ggctggtctt gcggccattg ccgcgccgcg atcatcgtca
     5641 acctgcgcga ttcgaaaatc gcgcggaaca gatccttggg aacctcggcg ccaaacgcat
     5701 cgtgtaattg gtcccagatg atcgcagcct ctgcgtcggt cttgataccg agatggtgct
     5761 tgaggatcgg gatcgagcct tgcttgttgt cgcggaagta catcacggcc ttggcaatgg
     5821 ctcggatgac cttcttcacc gtctcggggt tattgtcgac gttggcttgc atgccccaga
     5881 tcgtgccgcc agcccttgga atgtagtcac cggtccgcaa gagcaccttc agtccgacat
     5941 tgacggccgt ggcggcatgt gggatcgaga gtgacgctcc ctggatatcc ccgttgatca
     6001 gggcggcaat tcgctccggc tcaccctgga acgagatcac tttgtagtcc ttgccgacgt
     6061 ccatgtgaaa gaaacggtgc agcaccgctt cggcttcgtc gtagtcggct ccgcccgcac
     6121 gaccgtagcc gactatcttg cccttcaggt cctccggctt gttgatctcc ttgcggaccg
     6181 tgaccgtcca ttgcgacttg agcgactcgc cgacgatgta tttgatcttc gctccgctta
     6241 gcgccgcctg cgatccgccc gacggaatgg gggcgaaatc gaggttgccg gacaaaaccg
     6301 cagtgacctg ggccttgccc tgcaactcga cgaagcgagc atcgaggctt tcagacttga
     6361 agaaaccccg atccatcgcg acgtagctcg gaatgtgaaa gccgtcacca ccgatcacct
     6421 tgccaatgga gatcttggtc tgggcgtggg cggtggcgat cgcaaggcag agagccaagg
     6481 cggcggtgac gcgcacgagc aggcatcgga gcattcgcat ggggactctc ctgtcataga
     6541 gccggtcgaa gcgtgtagtc acctggcaat aagcggcttc gaatgccagt ctaggcacgg
     6601 ccgatctaag caagttgaag tgttccggca tcgttgcggc ttgtcaacca acggctcgtt
     6661 acgttgccga cgtgcgcgcc gcggcagagt gttgcctccg tgggtgtaaa ccctcgatat
     6721 ttggccgaat acgatccacg ccgggcacgc cgtcttcgaa accgcccgcg cgacgtggcg
     6781 cagaggtctt tcggggtgct caagccggac ggtcgcgcgg cgcttatcat ttctggcgca
     6841 caggcagcaa agcccaaacg cgatgatgcg tgactgtcgg ctcgtggcac ttcgcgacgg
     6901 atcgcatgat gatcaccgaa ctgtcgcttt cggggcgaag cggaagcgca cgcacacttg
     6961 gatcgaccgc ctgtggatca ttcgatcccc atcgtccgac cccttctgaa cgcgttttgg
     7021 cgccggtccg gcgaccgtgg caggcgtggt atccttcaaa ttgcgattgg cgcagtctga
     7081 ggctgtggga ggaccgcatg cgacgtcgtg atttcgtcac agggattgca ggcccggcag
     7141 tcgcttggcc gctcgctgct cacgcgggaa gatggccgac tcaggtggtg cgcatcgtct
     7201 gcccactcgc ggcgggtgga ggaatcgacg cgactgcccg tatcgtcgcc gcccgccttg
     7261 gcaccaacag gtcgtcgtcg agaacaagtc cggcgccagc ggcaacattg cggccgaatt
     7321 cgttgctgcc cgacttggct ttgccccgcc ccctacatga tcgaattttg gggatagggg
     7381 tggcctgcac tgcgtaacgc aactcatcaa ttcaatcaaa gttcagcatc gccaaccgct
     7441 gggcgatgaa gtcactaatc gcttgccaaa atgaagcctc tcgcggtcta agctcggctt
     7501 ctcgactgac cgcgttgtcg agctcctcca gcgcggctcg ttgcgcggca cgatttgttc
     7561 ctactggtgt aagaaaatcg cgaattctta gctctgccgt cggcagcctg tttgcttcca
     7621 actcagatga tcgagccagg gaccgcaggg cttccatgca tgtatcgagg cgagtactca
     7681 tgaccatgct caacgcgcat actccattgg ggatgtacta tacggcccta gtcacagacc
     7741 gcgccatgga accgtggtat aatccctcga ttgcatgaat gacccccaac cggagggtca
     7801 catggcaagc tacatccgac gacgcaaatt cttagccacg ctcggcggcg cggcggcgtg
     7861 gccgctcgcg gcgcgcgcgc agcagcgcaa acgctggagg cgcttgatat cttttgagcg
     7921 gcgggcttcc gatcattcct gtgactgaaa tgctacacta gacaactttt ttagaagttc
     7981 gggtccctat gctccacaat tgtcccaatt tttcttcaca aaaacagtcg cgagacgggg
     8041 acatggcaga cttaaggtcg aaggggtggt gcgatgcgcg aatcgtcggg aaatgaaatc
     8101 cctgacacgg ctccgtacat agaatggctg gcaatcgtct gcgccctcgt ggtggcggtt
     8161 ctggtttttg cgcaggccta ttctcaaatg gtatcctaac ctgagggtcc tcccttcggg
     8221 gaagtaaaag gctctacgtc agctcgctgc gagaagacag ctttggcctt tccgcagtga
     8281 acgccggcgg cacacggagg tgggcatgtt caagagcaaa gcacctgaga aggataagga
     8341 cgtggcagca gatacgaccg aagtcgttgg gaggacggtg caacagcaac agtcgactgt
     8401 tgaacagccc ccgccgaggc gagagccgac gacgaatgcg gcgatacgct cttgcatcgg
     8461 ctccggtatg tcgatcgtcg gcaatatcga atgcaacggc ccagcgcagg tcttcggccg
     8521 aatcgaaggc gaggtgcgcg cctctgatct tctaatcggc gatggcgcac agatcgaggg
     8581 cagcgtcatt gcgcaggacg tcacagtctg cggtcgcgtc aagggcacca tccgtgccgt
     8641 ccgcgtcaaa ctgcaagaca gtggtg
//
LOCUS       DATLJT010000217         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1389401,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000217 DATLJT010000000
VERSION     DATLJT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389401"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..768)
                     /locus_tag="VM910_04915"
     CDS             complement(130..768)
                     /locus_tag="VM910_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61919.1"
                     /translation="MLVTGTAHGLQYWKNKELLDEIVERPGEPLPDPETLNAQIPKTE
                     WGFDLNGEPQPPWRETWAAYLIDTASATEYTFINSTNGARIAVEQLGRRLEVTQGLRG
                     PNVRPRVKLDSRPMPIKRLNITKQRPEFVVLEWIDLTGSGEVLLGRSQQSPLQIEHKP
                     EPTPAPPEKKPKPTSPKKKEVGKPVKPATVSEEIDDGLPGDLAPPTNPLRAG"
     gene            complement(860..1156)
                     /locus_tag="VM910_04920"
     CDS             complement(860..1156)
                     /locus_tag="VM910_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61920.1"
                     /translation="MYPQILDDSIAAERALGIAAGLGWLRLAEAMVAYIDYGLSPGMR
                     AAMEAAEKLSLPIERRQLPRFKEFPQPRKSAQPKAPISSASQPTRCSNESNSQV"
     gene            complement(1264..1758)
                     /locus_tag="VM910_04925"
     CDS             complement(1264..1758)
                     /locus_tag="VM910_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVI61921.1"
                     /translation="MRILGIDPGVRGGLAIVEVNNGVAPKLVDAIDVPTVGIGAKERI
                     DAIAMRGWIQTYRPDHAAIERAQAAPKQGASSGFKYGRAVGTIEAAILCCEISMTIIE
                     PATWKRALHLRGKDKEGARQLALQRFPAAHALLARKRDHQRAGNRADCADCKLYNSVM
                     PCVV"
     gene            complement(1768..2067)
                     /locus_tag="VM910_04930"
     CDS             complement(1768..2067)
                     /locus_tag="VM910_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61922.1"
                     /translation="MKYFLNHISPTPAAIARALVSYAEEIRMIERVLRQLYPAEPPPE
                     DPLLFAAILAVHAMTFGVCAAEDRQREIEARAEKHVEHTRKLAEAITKSDKPKLQ"
BASE COUNT          388 a          667 c          716 g          494 t
ORIGIN      
        1 gacctcgagg tcgacggtac tgcgcgtttc gatatcgaag aacagcgtag gcatagcgcg
       61 ttgccgcggt gatcgcttct cgctgtcgcc caccccgaca tgagcgacag caggctgcga
      121 tcacgatcat caccctgccc gcagcggatt ggtcggtgga gcgagatcgc ccggcagtcc
      181 gtcgtcaatc tcctcggaga ccgtggcggg cttgacgggt ttgcctacct ccttcttctt
      241 cggcgatgtc ggttttggct tcttctctgg tggtgccggc gttggctccg gcttatgctc
      301 gatctgcaag ggggattgct ggctccggcc aagcaacact tcgccactcc cagtaagatc
      361 gatccattcg agcacgacga attcgggtcg ctgcttcgtg atgttcagcc gctttatcgg
      421 cattggccgt gagtcgagtt tgaccctcgg ccggacgttg gggccgcgca gcccttgtgt
      481 gacctccagc ctacggccca gctgttcgac cgcgatgcgt gcgccattcg tcgagttgat
      541 gaacgtatat tcagtagcgg aggcggtatc gatcaggtac gccgcccaag tctcccgcca
      601 cggcggctgc ggttcgccgt tgagatcgaa gccccactcc gttttgggaa tttgagcgtt
      661 gagcgtctct ggatccggca ggggttcacc gggccgttcg acgatctcgt cgagcagctc
      721 tttatttttc cagtactgca gcccatgcgc ggtcccggtc accagcattt cattggccgg
      781 atcgagaccg tcgcggtcct tccagcggcc gtccacgcat ttgaggatca cgcttccggg
      841 cttgattaga cgtggctttt caaacttggc tatttgattc attggagcac ctagtaggct
      901 gtgatgccga agagatcggt gccttcggct gcgcgctttt ccgcggttgc ggaaattcct
      961 tgaatcgggg cagctgccta cgttcaatcg gaagcgaaag tttctccgcc gcttccatcg
     1021 cggcacgcat gcctggggaa aggccgtaat cgatgtaggc caccatggcc tcggccaacc
     1081 gcagccagcc gagaccggcg gcgataccga gcgcacgctc cgcggcaatg ctgtcgtcca
     1141 ggatctgcgg gtacaaaagg tgcgaagcga tcggtgcttc gccgcgggct aggcaatcgc
     1201 gcaggcagcg gcgcgcatac gcaacgtgag cctcgatgtc gccagcgaaa ggggattcaa
     1261 ggattacacg acgcatggca tcaccgagtt gtagagcttg cagtcagcgc aatcagcgcg
     1321 gtttccggca cgctgatggt cacgcttgcg ggccagcaaa gcgtgtgcag ccggaaaccg
     1381 ttgcagcgcg agttggcggg cgccctcttt gtctttgccg cggaggtgaa gcgctcgctt
     1441 ccatgtcgcc ggctcgatga tggtcatcga gatttcgcag caaaggattg cggcctcgat
     1501 cgttccaacg gcacggccgt atttgaaacc ggagctcgcg ccttgctttg gcgccgcttg
     1561 ggcgcgctcg atcgcggcat gatcgggacg gtacgtctga atccagccgc gcatcgcgat
     1621 cgcatcgatg cgttccttcg caccgatgcc gaccgtcggg acatcgatcg cgtcgacgag
     1681 cttcggtgct acgccgttat tgacttcgac gatggcgagt ccgccacgca cgccgggatc
     1741 gatgccgagg attctcatgg catgccccta ttggagtttt ggcttgtccg acttcgtgat
     1801 tgcttcggcg agcttgcgtg tgtgctcgac gtgtttttcg gcgcgcgcct ctatttcccg
     1861 ctggcgatct tccgcggcgc atacgccgaa ggtcatcgca tggacggcaa ggatggcggc
     1921 aaacaggaga gggtcttccg gcggaggttc cgccggatag agttgtcgca gcacacgctc
     1981 gatcatgcga atttcttcgg cgtagctgac aagcgcgcgt gcgattgcgg ccggcgtcgg
     2041 ggagatatgg ttgagaaaat acttcacgga aacgacactc aatttggcgg ggtgcgctta
     2101 atgggaggcg tgactcgcac gcctccggtt aaggacatca gccgatcaga gagctttgac
     2161 gaggatgttc cagacggtct cggtgccgat ctcgcgtaca gcctcgacct gttgcggctc
     2221 ggtcatggaa tcccacaaca gcacgacgag cgtctcgggg cggag
//
LOCUS       DATLJT010000218         7410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2078776,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000218 DATLJT010000000
VERSION     DATLJT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078776"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VM910_04935"
     CDS             <1..585
                     /locus_tag="VM910_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVI61923.1"
                     /translation="MNHDAYSDDYIRGILNSVKSIAMVGASPVDVRPSYFAFKYLAQR
                     GYDMIPVNPGHVGKTLLGRPFVASLAEIGRPVDMVEIFRSSAHLMPVVEEALKLDPLP
                     KVIWMQLGGRDDKAAEKAEAAGIKVVMNRCPKIEYGRLSSEISWMGVNSRTISSKRAP
                     IPTQGMRLSLNRMSVGGGTTAASDRAAKNRNEAS"
     gene            792..2072
                     /locus_tag="VM910_04940"
     CDS             792..2072
                     /locus_tag="VM910_04940"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HVI61924.1"
                     /translation="MNDRVPGFSTLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHA
                     ASLFGLQAFGNIYTRIGNPTNAVLEERVAALEGGTAALAVASGHAAQVIVLQQLLKPG
                     DEFVAARKLYGGSINQFTHAFKSFGWNVAWADTDDIGSFETALSPRTKAIFIESIANP
                     GGTITDIEAVAAVARRAGVPLIVDNTLATPYLIKPIDHGADIIVHSLTKFLGGHGNSL
                     GGIIVDAGTFDWSKDNKYPMLSEPRPEYHGIRIQETFGNFAFAIACRVLGLRDLGPAL
                     SPFNAFLILTGIETLPLRMQKHCDNAKAVAEFLQGHPSVASVSYAGLPGDKYNILARK
                     YAPRGAGAVFTFNLKGGYQAGVNLVSNLKLFSHLANVGDTRSLVIHPASTTHSQLDDA
                     TKIKAGAGPDVVRLSIGIEDREDLIADLEQALTA"
     gene            2120..2521
                     /locus_tag="VM910_04945"
     CDS             2120..2521
                     /locus_tag="VM910_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61925.1"
                     /translation="MTWSEPMLRNAILLLLTVAFAAPAFGADQVIVPHPRGFARPNLP
                     PGLPRPHYSYRTTIVYGAPPPPPVYVEEPLISSFVWTTPLLPGSYTLPGYYGRPFDYY
                     YQGAYYGGPYGNGYEGYFYRLPYACGVTGYC"
     gene            complement(2518..3606)
                     /locus_tag="VM910_04950"
     CDS             complement(2518..3606)
                     /locus_tag="VM910_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HVI61926.1"
                     /translation="MTALPPKLADSQRAVRAWLFSVAALIAIMVLVGGATRLTESGLS
                     IVEWKPVTGTLPPLDQAQWEQAFEAYRQIPQYRQLNAGMTLSEFKTIFWWEWSHRLLG
                     RVIGVVYLLPFLYFLWRGGMGAELKRRLWLIFGLGALQGAVGWWMVASGLSERIEVSQ
                     YRLATHLVLALLIFAAVVWTLRRLSERPSLVVSLRLKITSAALLVLTFAQLYLGALVA
                     GLRAGLIYNTWPDIDGAFIPSGARLWFEQPWWRNLFENTLTVQFEHRMTAYALFAIAL
                     LHAFDAIVSRAGAAARGALWLAAAVTLQALLGILTLINQVPLAFALSHQAVAILVLTL
                     AVLQAERLAGRQTEVAPRDVAATLGQAS"
     gene            3701..3907
                     /locus_tag="VM910_04955"
     CDS             3701..3907
                     /locus_tag="VM910_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HVI61927.1"
                     /translation="MTIRTRKFFGTIALLVLAAVWSLMGMAVAQMPLLAASGLLQAVF
                     YVVVGIGWVLPAMPIVSWMSRPDA"
     gene            complement(3926..4981)
                     /locus_tag="VM910_04960"
     CDS             complement(3926..4981)
                     /locus_tag="VM910_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI61928.1"
                     /translation="MPSDNAFLKSLRLELAYFRGRAFWRSREVGGAGVILRFERVRPR
                     RTGRFQPLRASEVTPKFLDRALGALRRWRYEFVTLDEACRRAVIMSLNRRFVCLTFDG
                     CSKDLITSAYPIMARHSVPFTVYVPTAFPDGVGQAWWLALEKIIARESRVSLVIDRNE
                     RHFTIKTVEQKYELFDYLSNWLRRMAPPDLSAAINDLCKRYSVDLAALSREASMDWSD
                     LAKLAADPLVTIGCATVNYHALSNLKDPDALREMTMGRAVAETVFQRDLTHFAYPFGD
                     RASFRRAHVMMAEELGFHSAVSTIPGIVDIEGRTNLRALPRISWDGRLHSLRAMRVLL
                     SGAMFDPVMPTPSKSPI"
     gene            5114..6379
                     /locus_tag="VM910_04965"
     CDS             5114..6379
                     /locus_tag="VM910_04965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI61929.1"
                     /translation="MTMVAAIGSRTAEAPLRSRTSRIAKIDIISDLARAEAVWRGLEA
                     EASTPYQRFDFLAIWQREVGARQKTQPFIVVAYDSEGRPLLLMPLALRQRLGVRVASF
                     MGDKHATFNMALWDKAFASEAGASELAALIAGIRERAAVDVMLLTQQPKRWRDMVNPF
                     AVLPHQDSVNGCPVLNMNPGAPPTALISSSFRKRLRSKERKLENLRGYRCHVADSDSE
                     ITRLLDWFFLTKRLRMAEQRLPNVFADPVVEEFVRKACVKRLANGKRAIDIHALQCDE
                     EVIALFAGVADDDRFSMMFNTYTMSDHARYSPGLILIRNIIDHYAAQGYRTLDLGIGS
                     DEYKRMFCRDDEPIFDSVIPLSGRGKLASFAISATRRAKRWIKRNPALLRLAHRCAAF
                     FTSAWSAARSFSLKRHHYALKRAPGRSSA"
     gene            6468..6743
                     /locus_tag="VM910_04970"
     CDS             6468..6743
                     /locus_tag="VM910_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HVI61930.1"
                     /translation="MKYITHWKLPTGTVNAAIKRFLETGGAPPEGVKMLGRWHGMNGE
                     GFALSESTDPKAMYQWYAQWIDVIELTVTPCVEDAEAGPILASLPKR"
     gene            complement(6915..>7410)
                     /locus_tag="VM910_04975"
     CDS             complement(6915..>7410)
                     /locus_tag="VM910_04975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI61931.1"
                     /translation="GLLEPPSLIFAPIDFEHESLAEGLTRVGFRQDQPAFFQWLGVVH
                     YLTRDAVASTLEFISATSQAAVVFDYAEPFQNYPVERRANVMAMAESAAARGEPWLSL
                     FDPAELLQLLRNVGLEDVEDLGLPELVERFYGALSRDIKVGPGPHVVRACQHSGRANR
                     VPSI"
BASE COUNT         1480 a         2297 c         2236 g         1397 t
ORIGIN      
        1 atgaatcacg acgcctattc cgacgactac attcgcggaa ttctcaacag cgtgaagtcg
       61 atcgcaatgg tcggggcttc ccccgtcgat gtcaggccga gctatttcgc cttcaaatat
      121 ctcgcgcaac gcggctacga catgatcccg gtcaatcccg gacatgtcgg aaagacactg
      181 ctgggccggc ccttcgttgc ctcgctggcc gagatcggcc gccccgtcga catggtcgag
      241 atcttccgca gctctgcgca tctcatgccc gtggtcgagg aggcgctgaa gctcgatccg
      301 ttgccgaagg tgatctggat gcagctcggt gggcgcgacg acaaggcggc cgaaaaagcg
      361 gaagcggcag gaatcaaggt ggtgatgaac cgctgcccga agatcgaata tggcaggttg
      421 tcgtccgaga tttcctggat gggcgtcaat tcgcgcacga tcagctccaa gcgcgcaccg
      481 ataccgacgc agggcatgcg gctgtcgctg aaccggatga gcgtcggcgg cggcacgact
      541 gctgcgtcgg accgcgcggc aaaaaaccgg aacgaagcgt cgtgacgcaa ttgcgaaacg
      601 atcgtttcga agtcgcttga gctggagcac aaaaccagtg aaatcagaac aaaccatcaa
      661 gtcgccttga cggcggtttt tcctgacgcg attgtccctc cttgagagga tgcgcgcact
      721 cgctcctcgc ctatagatcg ccgcgcggcc gacacccgcg agacggcagc ttgtccatcg
      781 aaggaagaag aatgaacgat cgtgtcccgg gattttccac gcttgccgta cacgcaggag
      841 cacagcccga tcccacaacc ggcgcccgcg cgacgccgat ctatcagacc acgtcattcg
      901 tgttcaatga tgcggatcac gcggcgtccc tgttcggact gcaggcgttc ggcaacattt
      961 acacgcggat cggcaatccg accaacgccg tgctggaaga gcgcgtcgcg gcgctcgaag
     1021 gcggcaccgc agcccttgcg gtcgcctccg gtcatgccgc gcaggtcatc gtgctccagc
     1081 aattgttgaa gcccggcgac gaattcgtcg ctgcgcgaaa actctatggc ggttcgatca
     1141 accagttcac ccacgccttc aagagctttg gctggaacgt cgcctgggcc gataccgacg
     1201 acatcggaag cttcgagact gcactgagcc cgcgtaccaa ggcgatcttc atcgagtcga
     1261 tcgcaaatcc cggcggcacc attaccgaca tcgaggcggt tgccgcggtc gcccgcaggg
     1321 ccggcgtgcc gctgatcgtc gacaacacgc tggcgacgcc ctatctgatc aagccgatcg
     1381 atcacggcgc cgacatcatc gtgcactcgc tgacgaagtt tctgggaggc cacggcaatt
     1441 cgctcggcgg tatcatcgtc gatgccggca ccttcgactg gagcaaggac aacaaatatc
     1501 cgatgctgag cgagccgcgg cccgaatatc acggcatcag gatccaggag acgttcggca
     1561 atttcgcctt tgcgatcgcc tgccgcgtgc tgggcctgcg cgaccttgga cccgcgctgt
     1621 cgccgttcaa cgcgtttctg attctgaccg gcatcgagac gttgccgttg cgcatgcaga
     1681 agcattgcga caacgccaag gcggtcgccg aatttctgca gggtcatccg tcggtggcat
     1741 ccgtcagcta tgcaggcttg ccgggcgaca agtacaacat tctcgcgcgc aaatacgcgc
     1801 cgagaggcgc gggtgcagtg ttcaccttca acctcaaggg aggctaccag gccggcgtga
     1861 acctggtgtc gaacctgaag ctgttttcgc atctcgccaa tgtcggcgat acccgctcgc
     1921 tggtgatcca cccggcttcg accacgcata gccagctcga cgacgccacc aagatcaagg
     1981 cgggtgccgg gcccgacgtg gtccgcctct cgatcgggat cgaggaccgg gaggacctga
     2041 tcgcggacct cgagcaggcg ttaaccgcat agccatttgt taaccattca tccggcaaag
     2101 cgtggtatcg atggctctga tgacttggag cgagccgatg ctgcgcaatg caattctact
     2161 cctgctcacg gtcgctttcg ccgcgcccgc gttcggcgcc gaccaggtga ttgtcccgca
     2221 cccgagaggt ttcgcccgcc cgaatttgcc acccggcctg ccgcggccgc attacagcta
     2281 caggacaact atcgtttacg gcgcgccgcc tccgccgccg gtctatgtcg aggagcccct
     2341 gatctcttcg ttcgtctgga cgacgccgct gcttccgggc tcctatacgc tgcccggcta
     2401 ctatgggcgg ccgttcgatt attattatca gggcgcctat tacggcggcc cctatggcaa
     2461 tggttatgaa ggctatttct atcgcctgcc ctatgcctgc ggtgtgaccg gctattgcta
     2521 gcttgcctgg ccgagggttg ccgcgacgtc ccggggcgcc acttccgttt gtctgccggc
     2581 aagccgttcg gcctgcagca cggcgagcgt cagcaccaaa atcgcaaccg cctgatgcga
     2641 cagcgcaaac gcgagcggta cctggttgat gagcgtcagg atgccgagca gggcttgcag
     2701 cgtcaccgct gccgccagcc atagtgcacc gcgggccgca gcacctgccc gggaaacgat
     2761 cgcatcgaac gcatgcaaga gggcgatcgc aaatagcgca taggccgtca tgcggtgctc
     2821 gaattgcacg gtgagcgtgt tttcgaacag gttgcgccac cagggctgct caaaccagag
     2881 ccgcgcgccg gaaggaatga atgcgccatc gatatcgggc caggtgttgt agatgagccc
     2941 agcgcgaagg cccgcgacca gcgcgcccag atagagctgc gcgaaggtca gaacgagcaa
     3001 cgccgcgctc gtgatcttca gacgcaagga aacgacgagg gacggccgtt cgctgagtct
     3061 gcggagcgtc cagacgacgg cggcgaagat caacagcgcc agcaccagat gcgttgcgag
     3121 gcgatactgt gaaacctcga tccgctcgga caatccggag gccaccatcc accaaccgac
     3181 cgcaccttga agcgcgccga gaccgaagat cagccacagc cgccgcttca actcagcgcc
     3241 catcccgccg cgccagagaa aatagagaaa cggcaggaga tagacgacgc cgatcacgcg
     3301 gccgagcagc cggtggctcc attcccacca gaagatcgtc ttgaactcgg agagcgtcat
     3361 gcctgcattg agctggcgat attgcgggat ctgccgatag gcctcgaagg cctgctccca
     3421 ttgcgcctgg tcgagcggcg gcagcgtgcc ggtcaccggc ttccactcga cgatggagag
     3481 cccggattcg gtgagccggg tcgcgcctcc gaccagcacc atgatcgcga tcaaggccgc
     3541 gacggaaaat agccacgcac ggacggcgcg ctgtgaatca gccaattttg ggggaagtgc
     3601 ggtcatccca actattgctt gaaggaattt gcgcgcccct tatagtccgc ccctccccgc
     3661 gcgcaagccg cgcatgcgat gaactcccga catttccgtc atgacaatac gtacccgcaa
     3721 attctttgga acaatcgcct tgctggtact ggccgcggtg tggtcgctga tgggaatggc
     3781 cgtggcgcaa atgccgttgc ttgcggcgtc cggcctcttg caggcggtgt tctatgtcgt
     3841 tgtcggcatc ggctgggttt tgccggccat gccgatcgtg agctggatgt cgcggccgga
     3901 cgcttgagcg ctcaattcca gggcgctaga tcggggactt gctgggcgtc ggcatcaccg
     3961 gatcgaacat cgcgcccgag agcagcacgc gcatggcgcg cagagagtgc aagcgcccat
     4021 cccacgaaat tcgtggcagt gcgcgcagat tggtgcggcc ttcgatgtcg acgattcccg
     4081 gtatcgtcga taccgcgctg tgaaacccca gttcctccgc catcatgaca tgggcgcggc
     4141 ggaaggacgc gcggtcgccg aatggataag caaaatgcgt caagtcgcgc tggaacacgg
     4201 tttcagctac tgcgcggccc atcgtcatct cacgcaacgc atcggggtcc ttcagattcg
     4261 acaacgcgtg ataattgacc gttgcgcagc cgatggtgac gagtggatcg gcggcgagct
     4321 tcgcgagatc gctccaatcc atcgacgcct cgcgcgagag cgccgcaaga tcgacggaat
     4381 atcgtttaca gagatcattg atcgccgccg acagatccgg cggagccatc cggcgcagcc
     4441 agttcgagag gtaatcgaaa agctcgtatt tctgctcaac ggtcttgatc gtgaagtggc
     4501 gctcgttgcg gtcgatcacg aggctgacgc ggctttcacg cgcgatgatc ttttccagcg
     4561 ccaaccacca ggcctggccg acgccatcag ggaacgctgt gggcacgtag acagtgaacg
     4621 gcacgctgtg acgcgccatg atcggatagg ccgaagtgat cagatccttg gagcagccat
     4681 cgaaggtcag gcagacgaaa cgccggttca gcgacatgat caccgccctc cggcacgcct
     4741 cgtcgagcgt gacgaactcg tagcgccagc gcctcagcgc accgagggca cggtccagga
     4801 acttcggagt gacctcgctt gccctaagcg gttgaaaccg ccctgtgcgc cgtggcctta
     4861 cgcgttcgaa ccgcaggatt accccggccc cgccgacctc gcggcttcgc caaaacgccc
     4921 gaccgcggaa atatgcgagt tcgagcctga ggctcttcag gaatgcattg tcggatggca
     4981 atcgaaagtc ccttcccgcc ctcaggtgcg ccagcctggc ggcaccagtc attacccttt
     5041 gttgacactt ctttgcaaag gtcggctggg tcccaaacag cgtaaatttt tcgcttttca
     5101 agcaggtttt gcgatgacca tggttgccgc gatcggaagc cggacggctg aagcgccact
     5161 tcggtcacga acaagccgca tcgcgaagat cgacatcatc agcgacctcg cccgcgccga
     5221 agcggtttgg cgcggcctcg aggccgaggc ttccacgcca tatcagcgct tcgacttcct
     5281 tgcgatttgg cagcgcgagg tcggagcaag gcagaagacg caacctttca tcgtggtcgc
     5341 ttatgacagc gaaggacggc cgcttttgct gatgccgctt gcgctgaggc agcggcttgg
     5401 cgttcgcgtc gcctcgttca tgggtgacaa gcatgcgact ttcaacatgg cgctgtggga
     5461 caaggcattt gccagtgagg cgggcgcttc ggaactcgcc gcgctgattg cagggatacg
     5521 cgagcgcgcg gcagtcgacg ttatgttgct gacgcagcag cctaagcgtt ggcgcgacat
     5581 ggtgaacccg tttgccgttc tcccacatca ggattcggtc aatggttgtc cggtcctgaa
     5641 tatgaacccc ggcgccccgc caaccgccct gatcagcagc tcattccgca agcggctgag
     5701 gagcaaggaa cgcaaactgg agaaccttcg cggctatcgc tgccatgtgg cggacagcga
     5761 cagcgaaatc acgcggttgc tcgactggtt tttcctgacc aagcggctgc gcatggccga
     5821 acagaggcta cccaacgtgt tcgccgaccc cgtcgttgag gagttcgttc gcaaggcctg
     5881 tgtgaagcgg cttgcgaacg gcaagcgcgc catcgacatc catgcgctcc aatgcgacga
     5941 ggaggtgatc gcactgttcg ctggcgtcgc cgacgacgac cggttttcaa tgatgttcaa
     6001 cacgtacacc atgtccgacc atgcgcgtta cagtccgggc ttgattctga tccgcaacat
     6061 catcgatcac tacgccgcgc aaggctaccg taccctcgat ctcggtatag gatcggacga
     6121 atacaagcgg atgttctgca gggatgacga accgatcttc gatagcgtca ttccgctcag
     6181 cggacgcggc aagcttgcct cgttcgcaat ctcagccacc cgccgcgcca aacgctggat
     6241 caagcgcaat cccgcgctgt tgcgtcttgc gcaccgctgc gcagcgtttt tcactagcgc
     6301 ctggtcggct gcgcgctctt tttccttaaa gcggcaccat tacgccctca aaagggcgcc
     6361 cggccgatcc tctgcgtgaa ccttttggcg gtcgggcaga caaaggttta ctgggcttga
     6421 aatgcaaccg caccggcggt tgcattattt agtggggagg accttttatg aagtacatta
     6481 ctcattggaa gttaccgacg ggcacggtga atgctgcgat caagcgattc ctcgagaccg
     6541 gcggcgcgcc acccgagggg gtcaagatgc tcggacgctg gcatggcatg aacggcgagg
     6601 gatttgctct ctccgagtcg acggacccaa aagcaatgta tcaatggtat gcgcaatgga
     6661 ttgacgtgat cgagcttacg gtcacgcctt gcgtggaaga cgccgaggct gggcccatac
     6721 tggcatcgct gccgaaacgc tgatcttgga gcgccgccgg cggcccgatc cgattcgcac
     6781 ttgacgccat cacgctttgg ggccgccttg ttgacggccc cttttgattg caaaggtcga
     6841 gcgcggcagc gccgactact cggcgcgcgg gcgactaagg gcgggaattt cgatccctcg
     6901 ggccttgatc gttttcagat tgatgggaca cggttagcgc gccctgaatg ttgacatgcc
     6961 cgcacgacgt gcggaccggg tccaacttta atatcccggc tcagtgcgcc gtagaagcgc
     7021 tccactaatt cgggaaggcc gagatcttcc acgtcttcaa gaccgacgtt tcgtagcagt
     7081 tggagcaatt cggcgggatc gaacagactg agccagggct caccccgcgc tgccgcgctc
     7141 tcagccatag ccatgacatt cgcgcgacgc tcgacaggat aattctggaa cggctcggca
     7201 tagtcgaaaa cgacggccgc ctgtgatgtc gctgaaatga actccagggt ggacgcgacg
     7261 gcatctcttg tcagatagtg cacaacgcca agccattgga agaatgccgg ctggtcttgt
     7321 ctaaacccga ctcttgtcag gccttcggcg aggctttcgt gctcaaagtc tatcggcgca
     7381 aaaatgagcg agggcggctc aagcagcccg
//
LOCUS       DATLJT010000219         3813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2997589,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000219 DATLJT010000000
VERSION     DATLJT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997589"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VM910_04980"
     CDS             complement(<1..788)
                     /locus_tag="VM910_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HVI61932.1"
                     /translation="MHLPARLRTILLAIAMVVVSFFVSLKALDWIVPHSVPTAPVLAA
                     LPPLAPAPRSSIVVAPVAISLSAIREAAERAAPRNFAGKADNPVSQVLQNGDIGWTAS
                     RGPILANGAQDVLSLTTPLTGKLNVTGSVSAKASGAVGDAIGGLLGANAAAKIGAVNI
                     KALNASAEIKGNVVITSRPKLAAAWHVEPNLAAQVNLGDTTLLLAGAKVNVPAQVKPL
                     IDKTVGEQLDAVGARIRTDPTLERAARVQWAKACRSMPLQGSDAT"
     gene            complement(921..1622)
                     /locus_tag="VM910_04985"
     CDS             complement(921..1622)
                     /locus_tag="VM910_04985"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI61933.1"
                     /translation="MTNLTAFIATSRRVVAFGALAIGTFAFSGSAVAAPPTQPFPAPF
                     FLVPQTQMAPPPTVGAAPYQDDGSTSELPARLRRQVVTYPTREAPGTIIIDTPNTYLY
                     YVLGGGRAMRYGIGVGRDGFTWSGVQSITKKAEWPDWTPPPEMIARQPYLPRYMAGGP
                     GNPLGARAMYLGGTVYRIHGTNAPDTIGTHVSSGCIRLTNADVTDLYSRVNVGTKVVV
                     LSMSDRRADLGSAVR"
     gene            1896..2951
                     /locus_tag="VM910_04990"
     CDS             1896..2951
                     /locus_tag="VM910_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HVI61934.1"
                     /translation="MRILIATDAWHPQVNGVVRTLTSLSRAAANLGAQIEFLTPDGFP
                     SVPVPTYQSLRVAIPNRGEIARRIEAAAPDAIHIATEGPIGWSVRGYCRRHGLAFTTS
                     YTTRFPEYVAVRTGIPASWGYAVLRAFHGAAATTMVATNSLRQELTERGFRRLGAWTR
                     GVDTTLFNPDEPAKLDLPRPIFMTVGRVAVEKNLEAFLALDLPGSKVVVGDGPQRAEL
                     ERRFPQARFLGERTGKDLTSHMAAADVFVFPSLTDTFGVVQLEALACGTPVAAFPVTG
                     PLDVIADHPIGALDQDLRSACMRALTMSREACRNFALDRSWENSARQFIGNLSVLQPG
                     RSPHPAPVVAGGSPVRG"
     gene            2982..3671
                     /locus_tag="VM910_04995"
     CDS             2982..3671
                     /locus_tag="VM910_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI61935.1"
                     /translation="MAETMKLDDARDLDFDRDMVEQAYDRWAPVYDLVFGGVFSKGRD
                     AAIQATNKLGGRVLEVGVGTGISLPLYAPHVRIFGTDISEAMLKKAKRRVAELRLKNI
                     EGLAVMDAEKLEFPDNSFDVVMAQYVVTAVPNPEAALDEFARVLRVGGELIILTRVSA
                     DDGMRRFIEQRLQPVVRRLGFRTAEFAWSRYTTWLQGARGMELVERRLIPPLGHFSLV
                     RFRKIEIAAAA"
BASE COUNT          671 a         1196 c         1241 g          705 t
ORIGIN      
        1 gtcgcgtccg agccctgcag cggcatcgaa cggcacgctt ttgcccattg cacgcgcgcg
       61 gcacgctcga gcgtcggatc ggtgcggatt cgcgcgccca ccgcatcgag ctgctcgccg
      121 acggttttgt cgatcaacgg cttgacctgg gcgggcacat tcaccttcgc gcccgccagg
      181 agaagggtgg tgtcgccgag gttgacctgg gcggcgagat tgggctcgac atgccaggcg
      241 gcagcgagtt tcggtcgcga ggtgatgacg acgttgccct tgatctcggc gctggcattg
      301 agcgccttga tgttgaccgc accgatcttg gcggcggcat tggcaccaag caggccgccg
      361 atcgcgtccc cgaccgcgcc gctcgccttg gcgctcaccg aacccgtgac gttcagtttt
      421 ccggtcaggg gcgtggtcaa cgacagcacg tcctgtgcgc cattggcaag gatcggcccg
      481 cgcgaggcgg tccagccgat gtcgccgttc tgcagcacct gggaaaccgg gttgtccgcc
      541 ttgcccgcga aattgcgcgg ggcggcgcgc tcggccgcct cgcgaatggc cgacagcgag
      601 atggcgacag gtgccaccac gatcgagctg cgcggcgcgg gcgccaatgg cggcagtgcc
      661 gccaacaccg gcgcggtggg cacgctgtgc gggacgatcc agtccaaggc tttcaggctg
      721 acgaaaaagg agacgacgac catcgcgatc gcaagcagga tcgttctgag gcgcgccggc
      781 aggtgcatcg tccatcctgc atgaatcaag cgcttgattt tacagggccg ccgggggctg
      841 cgccagagcg tcggacttca aacaaaacgg gctccggcaa ggcgccggag cccgttgtca
      901 accttacgaa actttaatcc tcagcgcacc gcagagccga gatcagcgcg ccgatcggac
      961 atcgacagca cgaccacctt ggtgccgaca ttgacgcgcg aatagaggtc ggtgacatca
     1021 gcattggtca gccggatgca gccactggat acatgcgtgc cgatcgtgtc cggcgcgttg
     1081 gtgccgtgga tgcgatacac ggtaccgccg agatacattg cgcgggcgcc gagcggattg
     1141 ccgggaccgc ctgccatgta gcgcgggaga tagggctggc gcgcgatcat ctcgggcggc
     1201 ggcgtccagt ccggccattc cgctttcttg gtgatcgact gcacgcccga ccaggtaaag
     1261 ccgtcgcgac cgacgccgat gccatagcgc atcgctcgcc caccgccgag cacgtaatag
     1321 agataggtgt tgggggtatc gatgatgatg gtgccgggcg cttcgcgggt cggataagtc
     1381 acgacctgtc ggcgcagccg cgcaggcaat tcggaagtgc tgccgtcatc ctggtacggc
     1441 gctgccccca cggttggcgg gggcgccatc tgggtctgcg gcacgaggaa gaagggcgcg
     1501 gggaaaggct gggtcggcgg cgcagccaca gccgagccgg aaaatgcgaa tgtgccgatt
     1561 gcgagggcac cgaaggccac gacacggcgg gaggtcgcaa taaatgcggt cagattggtc
     1621 attgtcgtcc cctgtttgat cgccttttgt gactggcgtt gcgcccagcc ggctttcttt
     1681 ttcaacttcc ttaacgatga aaggtttcgg gacgtttgct cgatccgcgc aaaacggttt
     1741 cgtcggggta aggatttgtt tcatgaccgt ttcacgactc cttcagcgac cacccccaca
     1801 aaaaacatct gcaacagttt gatgatgtca cacgcctgaa atatccgtgc ccgattttcc
     1861 gcacctgaga atcatttcaa ctctcgggtt tgcatatgcg gatcttgatc gcgaccgatg
     1921 cctggcaccc gcaggtgaat ggcgtggtgc gcacgctgac gtcgctctcg cgcgccgcgg
     1981 ccaatctcgg agcccaaatc gaatttctga caccggacgg ctttccgtcg gtacccgtac
     2041 cgacctatca gagcctgcgc gtcgcgatac ccaaccgggg cgagatcgcg cggcgcatcg
     2101 aagcggctgc gccggacgcg atccacatcg cgaccgaagg cccgatcggc tggtcggtcc
     2161 gcggctattg ccgccgccac gggcttgcct tcacgacgtc ctacacgacg cgcttccccg
     2221 aatatgtcgc ggtccgcacc ggtattccgg cgagctgggg ttatgccgtg ctgcgcgcct
     2281 tccatggcgc cgccgccacg accatggtcg ccaccaattc gctgcggcag gagctaaccg
     2341 aacgcggctt caggcggctc ggcgcctgga cccgcggcgt cgataccacg ctcttcaatc
     2401 ccgacgagcc ggcaaaactg gacctgcccc ggccgatctt catgacggtc ggccgcgtcg
     2461 cggtggaaaa gaacctcgag gcgtttctgg cattggacct tccgggctcg aaagtcgtgg
     2521 tcggagacgg gccgcagcgc gccgagctcg agcgccgctt tccgcaagcg agattcctcg
     2581 gtgaaaggac cggcaaggat ctgacctcgc atatggcggc ggcggatgtg ttcgtgtttc
     2641 cgagcctcac cgacacattc ggtgtcgtgc agctcgaggc gctggcctgc gggacgccgg
     2701 tcgcggcctt tccggtcacg ggtccgctcg acgtgattgc cgatcatccg atcggcgcgc
     2761 tggatcagga cctgcgcagc gcctgtatgc gcgcgctcac catgtcgcgc gaggcgtgcc
     2821 gcaattttgc gctcgatcgc tcctgggaga acagtgcaag gcagttcatc ggcaatctct
     2881 cggtgctgca gccgggccgg tcgccgcatc cagcacctgt cgttgccggc ggaagtccgg
     2941 tccgcggctg atatgatcct ccaacgagag tgaaagttac catggcagaa acgatgaagc
     3001 ttgacgatgc aagggacttg gatttcgacc gcgacatggt cgagcaggct tacgaccgtt
     3061 gggcgccggt ctacgatctc gtgttcggcg gcgtgttctc caagggccgc gacgccgcga
     3121 tccaggccac caacaaactc ggcggccgcg ttctcgaggt cggtgtcggc accggcatct
     3181 cgttgccgct ctatgcgccg catgtccgga tcttcggcac cgacatttcg gaagcgatgc
     3241 tgaagaaggc caagcgccgc gtcgccgagc tgcgcctgaa gaacatcgag gggcttgccg
     3301 tgatggacgc cgagaagctc gaatttcccg acaattcctt cgatgtggtg atggcgcaat
     3361 atgtcgtgac cgccgtgccg aatccggaag cggcattgga cgagttcgcc cgcgtgctca
     3421 gggtgggcgg cgagctgatc atcctgacgc gcgtcagtgc cgacgacggc atgcgccgtt
     3481 tcatcgagca gcgattgcag cccgtggtgc gcaggctcgg cttccgcacc gccgagtttg
     3541 cctggtcgcg ctacacgacg tggctacagg gtgccagggg catggagctc gtcgagcgtc
     3601 gtctgattcc gccgctcgga catttctcgc tggtccgttt ccgcaaaatc gagattgctg
     3661 ccgctgcgtg agaccattcg cgccctgacg cccgcggcaa acataacggg cgtgtgacat
     3721 tatgatgatg tcacacaccg gacatcgagt tcgtgtaaac gtcatccgac tcacctttgg
     3781 gggaaaaaat gatcaagagc ttcctggagc agt
//
LOCUS       DATLJT010000220         1771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_470470,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000220 DATLJT010000000
VERSION     DATLJT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470470"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VM910_05000"
     CDS             complement(<1..168)
                     /locus_tag="VM910_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI61936.1"
                     /translation="MTTFVLVHGAWHGGWCYKRVARLLRQAGHEVYTPTLTGLGERAH
                     LMSRTIDLGTHV"
     gene            complement(201..1190)
                     /locus_tag="VM910_05005"
     CDS             complement(201..1190)
                     /locus_tag="VM910_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61937.1"
                     /translation="MRRRDFITLLGGAAAAWPLAARAQQPERMRRVGMLMTQAQDDPN
                     GRARNKAFLEALEKLGWSIGGNVRIEYRWSAGDAERIRKDAADLAALAPDVILTNGAA
                     GVGPLLQATRTVPVVFVLVADPVGAGFVDSLAHPGGNATGFSALEYGLGGKWLELLKE
                     IAPGVTRAAIVRDPAISAGIGLFGAIQSAAASLGVEVTPVDVRDITQIEHALAAFARV
                     PKGGVVVTASALALSRRDPLIALAAQYKLPAVYFTRDFVTAGGLAAYSPDSLEQYRRA
                     AGYVDRILKGEKPADLPVQAPTKYELVINIKTAKALGLTVPPTLLARADEVIE"
     gene            complement(1187..1375)
                     /locus_tag="VM910_05010"
     CDS             complement(1187..1375)
                     /locus_tag="VM910_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61938.1"
                     /translation="MTEALMMAHGFNSELLVDLCIAGLAIAKVERMIAGGRTVEVVRL
                     KITEAGRQALTASRGKST"
     gene            complement(1577..>1771)
                     /locus_tag="VM910_05015"
     CDS             complement(1577..>1771)
                     /locus_tag="VM910_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61939.1"
                     /translation="ESEITGFSESVMSRDYDLTIGMFTKDCKFDAESLATLKRSFVDL
                     KLLAMPPDMSALYTEAFLPK"
BASE COUNT          330 a          560 c          542 g          339 t
ORIGIN      
        1 gacgtgcgtg ccgagatcga tcgtgcggct catcaggtgg gcgcgttcgc cgagtccggt
       61 cagagtcggc gtatagacct cgtgcccggc ctggcgcaac agccgcgcca cccgcttata
      121 gcaccagccg ccatgccagg caccgtgaac cagaacgaag gtggtcatga gtccttttag
      181 attagcggaa ataactaggg ttactcgatc acctcgtcgg cgcgggctag cagcgtgggc
      241 gggaccgtga ggccgagcgc ctttgcggtc ttgatgttga tcaccagctc gtatttcgtg
      301 ggtgcctgta ccggaaggtc agcgggtttc tcgcctttga ggatgcggtc aacgtagccc
      361 gccgctcgcc ggtattgttc gagactgtcg ggtgaatacg cggcaagtcc gccagcggtc
      421 acgaaatcgc gggtaaagta tacggccgga agcttatatt gtgccgcaag tgcgatgagg
      481 ggatcgcgtc gactcaacgc caaagcgctt gccgtcacga ccacaccgcc tttcgggacg
      541 cgcgcgaacg cagcgagggc gtgctcgatt tgcgtgatgt cgcgcacatc gacaggggtc
      601 acctctacac ccaacgatgc tgccgcggac tggatggcgc cgaacagccc tatcccggcg
      661 gatatggccg ggtctcgaac aatcgcggcc ctcgtcacgc ccggagcgat ctctttcaac
      721 agttccagcc atttgccgcc taacccgtac tccagcgcgc taaaaccggt cgcgttaccg
      781 ccagggtgcg caaggctatc gacgaagccg gcaccaaccg gatctgcgac caacacgaac
      841 acgaccggga cagtgcgggt tgcctgtagc agcggtccta cacccgcggc gccgttggtc
      901 aggatgacgt ccggcgcgag tgcggccaaa tcggccgcat ctttgcgaat gcgctccgca
      961 tcacctgcgc tccatcgata ctcgatgcgc acattgccgc cgatggacca acccaatttc
     1021 tccaatgctt ccaggaacgc tttattgcga gctcgcccgt tgggatcatc ctgcgcctga
     1081 gtcatcaaca tgccaactcg ccgcatccgc tcgggctgct gcgcccgcgc cgcgagcggc
     1141 catgccgccg ctgcgccgcc gagtagcgtg atgaaatcgc ggcgcctcat gttgattttc
     1201 cccggcttgc tgtcagtgct tgccgccccg cctccgtgat cttcaagcga acaacttcaa
     1261 ccgtgcgtcc gccggcgatc atgcgttcga ccttagcgat cgcgagacca gcaatgcaca
     1321 gatcgaccag cagctcgctg ttaaagccgt gcgccatcat cagcgcttcc gtcatgcctt
     1381 gcggcgcgtc ggcgagtaga cgaagggcgc ggcgctgctg cgtacgttcc cgctgcgcac
     1441 gcgattgcat ggccgattgg cctccgtggc tcgagagttt gcaatgcttt acgagatcgc
     1501 ggcggccaag tcgaggcggg gaaaggacaa acgcgggtgg cggattggcg cccattaggg
     1561 cggccgtgga ccaccgttat ttcggcagga aagcctcggt atagagcgct gacatgtcgg
     1621 gcggcatggc caaaagcttg agatcgacga acgaccgttt gagggtcgca agcgattccg
     1681 cgtcgaactt gcaatccttg gtgaacatgc cgatcgtgag gtcgtagtcc ctcgacatca
     1741 cgctctccga aaatccggtg atttcgctct c
//
LOCUS       DATLJT010000221         4477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2997722,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000221 DATLJT010000000
VERSION     DATLJT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997722"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VM910_05020"
     CDS             complement(<1..882)
                     /locus_tag="VM910_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61940.1"
                     /translation="MATTIFDWRDLPFHEIWCVDTEFYPGAGLANGAVHGDAMTPLCL
                     VALEMRSGRLVRLWQDELGRFPPYRLDSDALIVGHMLAAEFGFHIACNWGEPARALDT
                     YIEFRHYVNDGTIKSGDREKGFFGIGGALRYFLEDEIDVVRKGEMRDRILQGPPFSAQ
                     EQRDIVAYCEDDVRALARLVPHIVPTIRLLSHAMFRAKFQWTIAQQERRGPPINRPLL
                     VRFRDHWEGMQLDLVTELDRPFGCYEIVDGKPHWRKERFAGYVRRNGMIWPRHESGAL
                     DETDQTFREMAGRYPQIE"
     gene            complement(863..1501)
                     /locus_tag="VM910_05025"
     CDS             complement(863..1501)
                     /locus_tag="VM910_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61941.1"
                     /translation="MAEESKKPTLEVVPSTPSDAQDIEALWLDPGLGDGITDAHWHDI
                     QIDKPRDYFRVHPDKTFRRKTEIYVHKSEDEINKQYYIIDPAMRGKIIEARICILVTC
                     IYRDGRPRLWPIPFPREGEKDNSAWSTARKAARAALEKWVKLVWVKKSYQTREALPGY
                     APDPDWSKLPTYNDMVKLAVGAHGIIRDTNHPMYRELVGAPAKAADDGDDDL"
     gene            complement(1559..1834)
                     /locus_tag="VM910_05030"
     CDS             complement(1559..1834)
                     /locus_tag="VM910_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI61942.1"
                     /translation="MNKHSPTNKGRRRRSAVRSDDAQYHSAAEFARRLGVGRTTIWRL
                     MRAGKLRYLRISATLVRIPVSEYERLGHQRRRAQLSERETTTTHHST"
     gene            complement(1953..2147)
                     /locus_tag="VM910_05035"
     CDS             complement(1953..2147)
                     /locus_tag="VM910_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61943.1"
                     /translation="MAIVRRRYPRDIPAGVTIADLHRLVANPETWKAECERRKINPPH
                     PPPPNRDTVARALRRASLIE"
     gene            complement(2281..3504)
                     /locus_tag="VM910_05040"
     CDS             complement(2281..3504)
                     /locus_tag="VM910_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI61944.1"
                     /translation="MARLMGRLTELQVRRLGRGWRNDGGGLYLRIVDKERRWWVFRYG
                     AGGKRYHGLGPLATVSLAEAREQARACRALLLKGEDPIAAGKAHRAAAALQAANAKTF
                     AECVEAYWEAHCASWTNKKHAREWKASLEAHVLPEIGSLPVASIGTTEVLRALQPAWA
                     KIPETASRLRTRIEAVLDWARVNGYRQGENPARWRGHLDHLLPARKKVARVKHHAALP
                     YRDVPAFLRQLRQRDDVDSRALEYLVLTAARAGEVVGAAWDEIDQTERDGWTWVVPAE
                     RMKARREHRVPLSRAARAVLEQTPRERRQGPIFAAVSGHTIWKYLRTLTDTATVHGFR
                     SAFRDWVAEQTTFPRETAELALAHRVGDDVERAYRRGDLLAKRWQLMEAWARYCASFP
                     TAGANVAWIRGGARG"
     gene            3918..4055
                     /locus_tag="VM910_05045"
     CDS             3918..4055
                     /locus_tag="VM910_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61945.1"
                     /translation="MAHLVDKRLVERANTMLLLGILWSALALCVLGALSYDIAHWFSA
                     W"
     gene            complement(4167..>4477)
                     /locus_tag="VM910_05050"
     CDS             complement(4167..>4477)
                     /locus_tag="VM910_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61946.1"
                     /translation="LGGETDHTVRRVVEFCDGWFPRPRAGWEPKRAVARLREAAVAAK
                     RDPATLSITVFNAPADRAALAPYREAGIARVLLEVPDLSREEILRRLDKNAPLVAENR
                     "
BASE COUNT          786 a         1397 c         1408 g          886 t
ORIGIN      
        1 ctcgatctgt gggtacctcc cggccatctc acggaaggtt tgatcggttt cgtcgagtgc
       61 tccgctctcg tgcctgggcc agatcatgcc gttacgtcgg acataaccgg cgaaccgctc
      121 tttacgccaa tgtggcttgc cgtcgacgat ctcgtaacag ccaaaggggc ggtccagctc
      181 ggtgaccaaa tcgagctgca tgccttccca gtgatcgcgg aagcgcacga gcaacggccg
      241 gttaattgga ggaccgcggc gctcctgctg cgcgatcgtc cattgaaact tggctcggaa
      301 catcgcatgc gacagcaacc ggatggtcgg aacaatgtgg gggacgaggc gtgcaagcgc
      361 gcgcacgtcg tcttcgcaat aggctacgat gtctcgctgc tcttgtgcgc tgaatggcgg
      421 cccttggagg atgcgatcac gcatttcccc cttgcgcaca acgtcgattt cgtcttcgag
      481 gaaatagcgc agcgccccgc cgataccaaa aaaacccttc tcgcggtctc cgctcttgat
      541 ggtgccgtcg ttcacgtaat gccgaaactc aatatacgtg tcgagagcgc gggccggctc
      601 gccccaattg cacgcaatat gaaagccgaa ctcggccgcc aacatgtggc caacgatcaa
      661 agcatcgctg tcgaggcggt agggaggaaa ccgcccgagt tcatcctgcc agaggcggac
      721 aagtcggcca gagcgcatct ccagcgccac caggcagagc ggcgtcatgg cgtcgccgtg
      781 gacggcgccg ttcgctaggc cggccccagg gtaaaactca gtatcaacac accatatttc
      841 gtgaaatggc agatcgcgcc aatcaaagat cgtcgtcgcc atcgtcggcc gccttcgccg
      901 gcgctcccac gagttcgcga tacatcggat ggttagtgtc gcggatgatg ccgtgcgcac
      961 cgacagcgag cttcaccata tcgttgtacg tcggaagctt gctccaatcc ggatcggggg
     1021 cgtagccggg tagggcctcg cgagtctgat atgacttttt gacccacacc aatttgaccc
     1081 acttctcgag cgcggcgcgt gcagccttgc gagctgtgct ccaagcagaa ttgtccttct
     1141 caccctcccg cgggaacggg attggccaca ggcgcgggcg gccgtcacgg tagatacaag
     1201 taacaaggat gcagatgcgt gcttctatta ttttgccgcg catcgcggga tcgatgatgt
     1261 agtattgctt gttgatctcg tcttcggact tgtgcacgta aatttcggtc ttgcgccgaa
     1321 acgtcttatc aggatgcacc cggaagtaat cgcgcggctt atcgatctga atgtcatgcc
     1381 aatgcgcgtc ggtgatcccg tcgcccaagc caggatcgag ccatagagct tcgatgtcct
     1441 gcgcgtcgct cggagtggac ggcaccactt caagcgtggg ctttttactt tcttcggcca
     1501 tggggaagcc tcctatcggc tgtggcttga atttgtccgt cgttactccg ccgagcgttc
     1561 aggtgctgtg gtgggtggtg gtggtttcgc gctcgctcaa ttgcgccctg cggcgctgat
     1621 ggcccaggcg ctcgtattca ctcacaggaa tgcgcactaa cgtggcgctg atgcggaggt
     1681 aacgcagttt gccggcgcgc atcaatctcc agatggttgt gcgaccgacg cctaacctgc
     1741 gcgcgaattc ggcagcggag tgatactgcg catcatccga ccggaccgcc gacctgcggc
     1801 gacgaccctt gtttgtcggt gagtgcttgt tcacgtgccg ctctcatcaa ctgagcggcg
     1861 caatccattt cacacttttt cgcgctggtc atttgcgcaa gtgcacgcaa gtgcacgcat
     1921 gcgtacgcaa atatacgcag gcgtaatttg cgtcactcaa ttaatgaggc gcggcgcaat
     1981 gcgcgggcaa cggtgtcgcg attaggcggt ggtgggtggg gcggattgat cttgcgtcgc
     2041 tcgcattcgg ctttccatgt ttctgggttg gcgacgagcc ggtgcaggtc ggcgatggtg
     2101 acgccagcgg gtatgtcgcg tggatagcgg cgccggacga tggccaagat catttgctcc
     2161 cgtagcgttg gttgcctctc cggctccggt tcgtccggcc attcgatctt gaccctttgg
     2221 atcggctcgc tgtcgtcaac ctcgatgatc ctgaccctag ccacgggtgg gaggtgcctt
     2281 ctagccacgg gcgcctcccc taatccacgc gacgttggcg cccgctgttg ggaagctcgc
     2341 gcagtagcgc gcccacgcct ccatcagctg ccagcgcttc gcgagaagat caccgcggcg
     2401 ataggcgcgc tcgacatcgt cgccaacgcg atgtgcaagt gcgagttcgg cggtctcgcg
     2461 cgggaaggtg gtttgctcgg cgacccaatc acggaaggcg ctacggaagc cgtgcacggt
     2521 ggcggtatcg gtcagcgttc gcaggtattt ccagatcgta tggccgctaa ctgcggcgaa
     2581 gatcggcccc tggcgacgct cgcgcggcgt ctgttccagc acggcgcgcg cggccctcga
     2641 caagggcacc cggtgctcgc ggcgggcctt catgcgctcc gctggcacga cccaggtcca
     2701 gccgtcacgc tcggtctggt cgatctcgtc ccaggcggcg ccgacgacct caccggcccg
     2761 cgccgcggtc aggacgaggt attcgagcgc gcgcgagtcg acatcgtcgc gctggcgtag
     2821 ctgccgcaga aaggccggaa cgtcccgata gggcagcgcg gcatggtgct tgacgcgcgc
     2881 caccttcttg cgagcgggca atagatgatc aaggtggccg cgccagcggg cggggttctc
     2941 gccttggcgg tagccattga ccctcgccca atcgagcacg gcttcgatcc gggtgcgcag
     3001 gcgcgaggca gtttccggga ttttagccca tgctggctgg agggcgcgca gcacttccgt
     3061 cgtgccgatc gaggccaccg gcaagcttcc gatctccggg agcacgtgtg cttctagcga
     3121 ggccttccat tcccgggcgt gcttcttgtt ggtccacgac gcacaatgtg cttcccaata
     3181 cgcctcgacg cactcggcaa atgtcttggc gttggcggct tggagcgcgg cagcagcacg
     3241 gtgggccttg ccggcagcga tcgggtcctc gcccttgagc aacagcgccc ggcaggcgcg
     3301 ggcttgctcg cgcgcttcgg cgagcgagac ggtggccagg ggacccagtc cgtgatagcg
     3361 cttgccgccg gcaccgtagc ggaacaccca ccagcggcgc tctttgtcca caatccgtag
     3421 atacagccca ccgccgtcgt tgcgccagcc gcgtccgagc cgtcggacct gcagctcggt
     3481 caaccttccc atcaggcgcg ccatgggctt tgtatccaca ctttcgtcca cactcacgac
     3541 gaaaaagcct gaaaaggtct gaaatgtcaa gaaaaagcta caaggtacgg tcaacgcgat
     3601 ttttcaggca ttttggcaac ggcgccaaag gcttgcgcgg gcactcgtgg cggagcctcg
     3661 ctccgccttc gggatcgtct ccggagacgc ccctcgatga gcgagactgg gcatctctag
     3721 catgggcgcg ttacgtagtc aatagtgtag ttgatgtgta gtcaaaaaaa ggggagtgcg
     3781 gcatcgtacg caaatgcacg gttaggggag gggaaacccg ctttccaaaa agcgcacttt
     3841 tgtgacgtac atcacagtcg caggcccgcg acgtgcctac ttttcgagga ggagggcggt
     3901 catagatgga ggctatcatg gcccatcttg tcgataagag actggtcgag cgcgcgaaca
     3961 ccatgctgct gctgggcatt ctgtggagcg cccttgcgct ctgcgtgctc ggcgcgctgt
     4021 cctacgacat cgcccactgg ttcagcgcct ggtgacatac atcctggcgc taacgaaagc
     4081 gccaagccat cggccggccc ggcctcgctc gagaccacga gtgggaattt tagaagccgc
     4141 gtagtcctgc ccgcatttcc ccgcgatcag cgattctccg ccaccagcgg cgcgttcttg
     4201 tcgagccggc gcaggatttc ttcgcggctc aagtcgggca cttcgagcag cacccgcgcg
     4261 atgcccgcct cgcgataggg cgcgagcgcg gcccgatcgg cgggcgcgtt gaacaccgtg
     4321 atcgagagcg tcgcgggatc gcgtttggcc gcgaccgcgg cttcgcgcag ccgcgccacc
     4381 gcgcgcttgg gctcccagcc ggcgcgcggg cgcgggaacc agccgtcgca gaactcgacc
     4441 acccggcgca cggtgtggtc ggtctccccg cccagca
//
LOCUS       DATLJT010000222         8953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1160050,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000222 DATLJT010000000
VERSION     DATLJT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160050"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /gene="trbB"
                     /locus_tag="VM910_05055"
     CDS             <1..888
                     /gene="trbB"
                     /locus_tag="VM910_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HVI61947.1"
                     /translation="IARFLEDPAVVEVMLNPDGRLWIDRLSSGLVDTGEMVSAADGER
                     IVRLVAHHVGAEVHAASPRVSAELPETGERFEGLLPPVVAGPAFAIRKPAVAVFTLDD
                     YVAAGIMTSGHASALKGAVEARKNILVAGGTSSGKTTLTNALLAEIAKTSDRVVLIED
                     TRELQCKAPNLVALRTKDGVISLSDLVRSSLRLRPDRIPIGEVRGAEALDLLKAWGTG
                     HPGGIGTIHAGTALGALRRLEQLIQEAVATVPRALIAETINVIAVLSGRGADRRLAEL
                     ASVEGLGSFGDYSLASIGD"
     gene            960..1217
                     /locus_tag="VM910_05060"
     CDS             960..1217
                     /locus_tag="VM910_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HVI61948.1"
                     /translation="MGPALAAGSNMPWEQPLNQILQSVEGPVAKIIAVIIIIVTGLSL
                     AFGDTSGGFRRLIQIVFGLSIAFAASSFFLSFFSFGGGVVI"
     gene            1217..1480
                     /locus_tag="VM910_05065"
     CDS             1217..1480
                     /locus_tag="VM910_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HVI61949.1"
                     /translation="MAGEIPGFVAPVHRALTEPILMGGAPRSIAIVNGTLAAALGIGL
                     RLWVAGLLFWLVGHMVAVWAAKHDPDFVEVVRRHLRIPGHLNT"
     gene            1488..3932
                     /gene="trbE"
                     /locus_tag="VM910_05070"
     CDS             1488..3932
                     /gene="trbE"
                     /locus_tag="VM910_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HVI61950.1"
                     /translation="MMNLAEYRRSNTRLADFLPWAALVDEGIILNKDGSFQRTAKFRG
                     PDLDSAVPAELVAVAGRINNALRRLESGWAVFVEAQRHSAGAYPPNTFPDVASALVDA
                     ERRAQFEETGAHYESSYFLTLLYLPPAEGTAKAERLLYEGSNRTVDADAREVLRGFVD
                     RTNRVLQLVEGFMPECAWLNDEETLTYLHSTVSTKRHRVRVPEIPMYLDSLLADRPLT
                     GGLEPMLGSAYLRVLTVVGFPTATTPGILDDLNRLAFAYRWSTRAIMLDKTDATKLLT
                     RIRRQWFAKRKSVAAILKEVMTNEASALLDTDAHNKAMDADEALQELGSDQIGEAFVT
                     ATISVWDVDPATADEKLRLVEKVIQGRDFTCMIETVNAVEAWLGSLPGHVYANVRQPP
                     ISTLNLAHMIPLSAVWAGEARDHHLKAPPLFLAKTEGSTPFRFSLHVGDVGHTLVVGP
                     TGAGKSVLLALMALQFRRYPNSQIFAFDFGCSVRAATIAMGGDWHDIGGVLSGESTEF
                     VELQPLAAIDEPSERGWAAEWITAILGREKVDVTPEAKDHLWSALTSLASAPIPERTL
                     TGLSVLLQSNVLKRALQPYCLGGPHGRLLDAEFERLGREGSVQVFETDGLIGTAVAPA
                     VLSYLFHRIEGRLDGRPTLLIIDEGWQALDNQEFAGQLREWLKTLRKKNTSVVFATQS
                     LADIDGSAIAPAIIESCPTRILLPNDRAIEPQITAIYRRFGLNDRQIEILARATPKRD
                     YYCQSRRGNRLFELGLGEVALAFTAASSKADQVLIDRVLAEDGRDNFVPGWLRARDIS
                     WATDLIPHLTELEASK"
     gene            3929..4663
                     /gene="trbJ"
                     /locus_tag="VM910_05075"
     CDS             3929..4663
                     /gene="trbJ"
                     /locus_tag="VM910_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HVI61951.1"
                     /translation="MNRLQRLAIASIVAITVAASLTPASAQLVVFDPNNYAQNVLTAA
                     RTLQQINNQIVSLQNQAQMLINQAKNLATLPFSSLLQLEQSIQRTQQLLAQAQRIAYD
                     IQQIDRAFSTTYAPASASVSNQALIAKAQTRWQNALVSLQDAMRTQATVVGNLNTSRT
                     QMSALVTASQGATGALQASQAGNQLLALQAQQLADLTAVVAAHERAQNLESAQHAAAQ
                     DQGREQLRRFLAPGQGYQSITVQMFH"
     gene            5051..6256
                     /gene="trbL"
                     /locus_tag="VM910_05080"
     CDS             5051..6256
                     /gene="trbL"
                     /locus_tag="VM910_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HVI61952.1"
                     /translation="MGGTGIIDQFLETFTRYIDSGFGLLGGEVGYLATTLAAIDVTLA
                     GLFWAWGADEDILSRLVRKTLFVGVFAFLIGNWNSLARIVFESFVGLGLKASGTGLSS
                     ADFLRPGKIAQVGLDAGRPLLESALGLMGYVGFVENFIQIVLLLFAWIVVLLAFFILA
                     IQLFITLIEFKLTTLAGFVLIPFGLFGKTAFAAERVLGNVISSGVKVLVLAVIVGIGS
                     TLFAQFTAGLGGNQPTIDNAMALVLGALSLLGLGIFGPGIANGIVSGGPQLGAGAAVG
                     TGLAAGGAIVAGGGLVAGGAGLAGGALLGGARGAATLVTGTASAYRAGGLSGVASAGG
                     SAIVSPLRRMADGLTRSSPTADASPAWARRMKRAQTVNQGVSAATHAVRSGDSAGGGA
                     SVDLSEGGQ"
     gene            6256..6939
                     /gene="trbF"
                     /locus_tag="VM910_05085"
     CDS             6256..6939
                     /gene="trbF"
                     /locus_tag="VM910_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HVI61953.1"
                     /translation="MFKRPTVHYGRTPEPVTPYQSAAQAWDERIGSARVQAKNWRLMA
                     FGCLLLSGGLAAALVWQSTQGTVTPWVVEVDHLGQAQAVAPASPFYQPTDPQIAFHLA
                     RFIEDVRGLPADAVVLRQDWLRAYEFTTDRGAAALNDYARTNDPFAKLGNMQVAVEIS
                     SVIRASPESFRVAWTERRYDNGQLAATERWTAILSVVIETPRDADRLRKNPLGVYVNA
                     INWSKELGS"
     gene            6948..7910
                     /gene="trbG"
                     /locus_tag="VM910_05090"
     CDS             6948..7910
                     /gene="trbG"
                     /locus_tag="VM910_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HVI61954.1"
                     /translation="MSIPASNGARVRLALFFLLALSGCTTFKPPQISYDDDVPPSPDL
                     PAPADDHARPLHVPPAWTPARGGNKGEAEAKDPTDRIEIANDVARVQPRRAGYFNAVQ
                     IFPYSPGALYQIYASPGQITDIELEPGEQLTGSGPLAAGDTVRWIVVDTESGNGDTRR
                     VHVMAKPTRPAIETNLVVNTDRRTYLIELRSREKAYMPSVAWFYPENRSGHSKPLPPT
                     PFIPEVEQRRYRYMIEGDNPPWRPVNAYDDGRKVYIEFSPGIGQREMPPLFVIGQDGK
                     PELVNYRVYGNVLIADRLFAAAELRLGADKQQRVRIVRNNGRRS"
     gene            7907..>8953
                     /locus_tag="VM910_05095"
     CDS             7907..>8953
                     /locus_tag="VM910_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HVI61955.1"
                     /translation="MTGNAPNESLGPAAGPTPSSEEPAHALRLRAERPRITRLSRKVL
                     AGGTALALLLISGAVLWALRSSHPHNPAPDELYSTDHHNIADGLTTLPHDYAGIARNV
                     PQLGPPLPGDLGRPIVTAEGQSTPVGLDAEQQRADQETEAARTSKLFAPTTALVTPPR
                     APSQETATKTASSSDETFAQNGQDRKLLFVNAPVDRRTTAPDRLSRPASPFVIQAGTI
                     IPAALITGICSDLPGQISAQVTEAIFDSPTGRFKLVPQGARLIGIYDSQVAFGQSRVL
                     LVWTRLIMPNGRSIVLERQQGADAGGYSGLEDEVDNHWKELLGAAALSTLLGVGSELG
                     SGADNGNNTAILQAL"
BASE COUNT         1660 a         2780 c         2688 g         1825 t
ORIGIN      
        1 atcgcgcgct tcctcgaaga tcccgccgtc gttgaggtaa tgctcaaccc tgatgggcga
       61 ttgtggatcg accggctgtc gagtggtttg gtagataccg gagaaatggt gtctgccgcg
      121 gacggcgagc gcatcgtccg attggtcgcc caccatgtgg gtgccgaagt ccacgcagcc
      181 agcccacgtg tttcggccga actgcctgag acgggggagc gcttcgaggg gttattgccg
      241 ccggtggttg cgggacccgc ctttgctatc cgcaagcctg cagtcgccgt gttcacgctt
      301 gacgactacg tcgccgccgg aatcatgact tcgggccacg ccagcgcttt gaaaggtgcg
      361 gtcgaggcgc gcaagaacat tcttgtggct ggcggcacct ccagcggcaa gacgacgctg
      421 accaacgccc tgctcgctga aatcgccaag acctcggatc gagtcgttct catcgaagac
      481 acgcgggagc ttcagtgcaa ggcgccgaac ctcgtggcgt tgcgcacgaa agacggtgtc
      541 atctcgctgt cggatctcgt gcgatcttcg ttgcgtctgc ggcccgaccg catcccgatc
      601 ggggaggtac gcggcgccga agcgctcgat ctgctcaagg cttggggcac gggtcatccc
      661 ggcggcatcg gcacaattca cgccggtacc gcgcttggcg ccttgcgccg gctcgaacag
      721 cttatccagg aagccgtcgc caccgtccca cgtgccctga tcgcggagac tatcaacgtc
      781 atcgccgtcc tctcaggtcg cggtgccgat cgccgactgg ccgagctcgc gagtgtcgag
      841 gggctcggat cgtttggtga ctacagcctt gcatcaatag gggactaatc atgatttttg
      901 ccacttcgcg cgagcgaggg atcgtttcga cgaccgtagt cgcgaccacg tatctgacga
      961 tgggtccagc cttggctgcc ggttccaaca tgccgtggga gcagccgctc aaccaaatcc
     1021 tgcaatcagt cgaagggccg gtcgctaaga tcatcgcggt catcatcatc atcgtcaccg
     1081 ggctgtcgct cgcgttcggc gatacatcag gcggattccg caggctgatc cagatcgtat
     1141 tcggattgtc gatcgcgttt gcggcgtcga gcttcttcct gtcgttcttt tctttcggcg
     1201 gtggagtggt gatctgatgg ccggtgagat tccaggcttc gtcgcgccgg tacatcgcgc
     1261 gctgaccgaa ccgatcctca tgggcggcgc gccgcggtct atcgccattg ttaacggaac
     1321 actcgccgct gcactcggca tcggtctgcg cctgtgggtc gcgggccttc tgttttggtt
     1381 ggtcggccac atggtcgccg tctgggccgc caagcacgac cctgactttg tcgaagtcgt
     1441 ccgccggcac cttcgtattc ccggtcatct caacacgtga gtctcccatg atgaatctcg
     1501 ccgaatatcg ccgttccaat acccggctcg ccgatttttt accgtgggcg gctcttgtcg
     1561 atgaggggat catcctgaac aaggatgggt cgtttcagcg caccgcgaaa tttcgaggac
     1621 ccgacctcga tagcgccgtg ccggcagagc ttgttgctgt tgccggccgt ataaacaatg
     1681 cgctgcgtcg gcttgaatcc gggtgggcgg tctttgtcga ggcgcaacgt cactcggcag
     1741 gcgcttatcc acctaacacg tttccggatg tcgcgtccgc cttggtcgat gctgagagaa
     1801 gggcacagtt tgaggagacg ggggcgcact acgaatcgag ctatttcctg acgcttctct
     1861 atttgccgcc ggccgaaggg acagcgaaag cggaacggct gctttacgag ggcagcaatc
     1921 gcaccgtcga tgctgatgcg cgcgaggtcc tccgcggttt tgtcgaccgt actaaccgtg
     1981 tgctgcagct cgtcgaaggg ttcatgccgg agtgcgcctg gctcaacgat gaagagacgc
     2041 tcacttacct gcactcgacg gtctcgacca agcgccatcg tgtcagggtg cccgagatcc
     2101 caatgtatct cgattcactg ctcgcagatc ggccactgac cggcggcctc gagccgatgc
     2161 tcggttcagc gtacctacga gtcctgacgg tggtggggtt tccgacggcg acgacgccag
     2221 ggattctcga cgacctgaac cgcctcgcgt tcgcctatcg ttggtccact cgcgcgatca
     2281 tgctcgacaa gacagacgca acaaaactct tgaccaggat caggcggcaa tggttcgcca
     2341 aacggaagtc ggttgccgcg atcctgaagg aggtcatgac caacgaggcc tctgctcttc
     2401 ttgatacgga tgcccacaac aaggccatgg atgcggacga ggcgctgcag gaattgggtt
     2461 ctgaccagat tggagaagct ttcgtcacgg cgacgatctc agtctgggat gtcgatcctg
     2521 ccactgccga cgaaaagctc cgcctcgtcg aaaaggtgat tcaggggcgg gatttcacct
     2581 gcatgatcga gacggtcaat gccgtcgagg cctggctcgg aagcctgccg gggcacgttt
     2641 acgccaacgt ccgccagccg ccgatctcga cgctgaattt ggcacacatg atcccgttgt
     2701 ccgcagtgtg ggccggggag gcgagggacc atcatttgaa ggctccgcct ttgtttcttg
     2761 ccaagacgga gggatcaact ccatttcgct tctcgcttca cgtcggcgat gtcgggcata
     2821 cgttggtggt cgggccaacg ggcgccggca aatcagtgct gctggcgctg atggcgctcc
     2881 aatttcgtcg ctatccgaac tcccagatct tcgccttcga ttttggctgc tctgttcgtg
     2941 ccgccaccat cgccatgggt ggcgattggc atgacatcgg cggcgttctc tcgggcgaat
     3001 ccacggaatt cgttgagctt cagccgcttg cggcgattga tgagccatcc gagcgcggtt
     3061 gggccgcaga gtggatcacc gcgatcctcg gccgtgagaa ggttgatgtc acgccggaag
     3121 ccaaggatca tctctggtcg gcactgacct cgctagcctc ggcaccgatc ccagagcgga
     3181 ccctgacagg tcttagtgtt cttcttcagt cgaacgtatt gaagcgcgct ctccagcctt
     3241 attgtcttgg ggggccgcat ggccggcttc ttgatgcaga attcgagcgt cttggccgcg
     3301 aaggctccgt tcaggtcttt gaaaccgacg gattgatcgg tacggcggtc gcgccggccg
     3361 tcctttccta tctgttccac cgcattgaag gacggttaga tgggcggcca actctgctca
     3421 tcatcgatga agggtggcag gcgcttgata atcaggaatt tgctggtcag ctgcgtgagt
     3481 ggctgaagac ccttcgcaag aaaaacactt ccgtcgtctt cgctacccaa tcgcttgccg
     3541 acatcgatgg ctcggcgatt gcgccggcca tcatcgagag ctgtccgacc cgaattctgt
     3601 tgccgaacga ccgtgcgatt gaaccgcaga tcaccgcgat ctaccgacgt ttcgggctca
     3661 atgaccggca gatcgaaatc ctcgctcggg caacgccaaa gcgtgactac tactgccaat
     3721 cgcgccgtgg caatcggctg ttcgagctcg gtctcggcga ggtcgcgctt gcctttaccg
     3781 ctgcttcctc caaggccgac caggtcttga tcgaccgggt gcttgccgaa gatggccgcg
     3841 acaactttgt gcccggctgg ctcagggcac gcgacatctc ctgggcgacc gacctcatcc
     3901 cgcatcttac cgaattggag gcgtccaaat gaaccgcctt cagcgtctcg ccattgccag
     3961 cattgttgcg atcaccgttg ccgccagctt gacgcccgcg tcagcgcagt tggttgtttt
     4021 cgatcctaat aactatgcgc agaacgtgct gacggctgct cgcaccctgc agcagatcaa
     4081 caaccagatc gtatctctgc aaaatcaggc gcagatgctg atcaaccagg cgaagaatct
     4141 ggcaactctg ccgttttcgt cgttgttgca actggagcaa tcgatccaac gaacccagca
     4201 gcttctcgcc caggcccagc gcatcgctta cgacatccag cagatcgatc gtgcattctc
     4261 gacgacctac gcgcctgcat cagctagcgt ttcgaaccaa gcgttgatcg caaaagcgca
     4321 gacgcgttgg cagaacgcgc ttgtcagcct gcaggacgcc atgcggacgc aggcaaccgt
     4381 cgttggcaat ctcaatacca gccgtacgca gatgtctgcg cttgtcactg cgagccaggg
     4441 cgcgaccggt gccctacagg cgagccaggc cggcaatcag ctcctggcct tgcaggccca
     4501 gcagctagcc gatctcacag cggtcgtcgc ggctcatgag cgcgcgcaaa acctggagtc
     4561 tgctcagcat gccgccgcgc aggaccaggg cagggagcaa ctgcgccgct tcctggcccc
     4621 gggccaaggc tatcaatcca taacagtgca gatgtttcac tgatgaagaa gacaacgctc
     4681 gataggctgc cgatgatggc agccatggtg ctcgtcgtcc tgatcgtcgc ggcctgcgca
     4741 attcgcctgc gtgacgacga aaaccaaaac acgtccgcca cgtcctttga ccaagcgtcc
     4801 gatcctctga caacaaagct cgcggagtgc cgctccgtga cctatgagca aaaggacgct
     4861 ctgtccgagt gccggaaggc ttgggccgaa aaacgccgcc aattcctcag gcgaacccct
     4921 ccgacaccac cagaaagagg gacgtctcaa ggcgcctccc cattgttcgt cctgcctgag
     4981 gacggcagcg cgccaaggct cggaccgcct aatcacaatt aaattccgca gtctggaaag
     5041 gaataggcgc atgggcggca ccggcattat cgatcagttc ctggagacct ttacccgcta
     5101 catcgattcc ggattcgggc tgctcggggg tgaagtcgga tatctcgcca ccacgctcgc
     5161 ggcgatcgat gtcacgctgg ccggcctgtt ctgggcgtgg ggcgcggacg aggacatcct
     5221 gtcgcgcctc gtcaggaaga ccctctttgt cggcgtcttt gctttcctga tcggcaattg
     5281 gaacagcctg gcccgcatcg tcttcgaaag ttttgttggt ttgggtctga aagcctccgg
     5341 caccggcctt tcctcggccg atttcttgag gcccggaaag atcgcccagg ttggccttga
     5401 tgccggacga ccactcctcg aatcggccct ggggctgatg gggtatgtcg gcttcgtcga
     5461 gaatttcatc cagatcgtgc tgctgctgtt cgcctggatc gtcgtgctgc tggctttctt
     5521 tattctggcc atccagcttt tcatcactct tatcgagttc aagttgacga cgcttgccgg
     5581 ctttgtgctg atcccattcg gcttgttcgg taaaaccgcc tttgccgccg agcgcgtact
     5641 cggcaacgtg atttcgtcgg gcgtgaaggt cctagtgctg gccgtcatcg tcggcatcgg
     5701 gtcgacgctg tttgcgcagt tcacggcagg ccttggcggc aatcagccga ccatcgacaa
     5761 cgccatggcg ctggtgctgg gggcactttc tcttctcggc ctcggcatct ttggacccgg
     5821 catagctaac ggcatcgtgt ccggcggacc acaactcggt gccggcgcgg ccgtcggtac
     5881 cggtcttgcc gcggggggcg ccatcgtggc aggcggcggt ctcgtagctg gcggcgcggg
     5941 actggcaggc ggcgcgcttc taggcggcgc ccgtggggca gccacgctgg tgacgggaac
     6001 ggcaagcgcc taccgagctg gaggcttgag tggtgtcgct agcgcaggcg gctcggccat
     6061 cgtcagtccg ctccgccgca tggcggacgg attgacaagg agttcgccaa ctgcagacgc
     6121 gtctccggct tgggcacgcc gcatgaagcg cgcgcagacc gtgaaccagg gcgtgtctgc
     6181 tgccacccat gctgtccgta gtggcgatag cgcaggtggc ggagcctcgg tcgatctgtc
     6241 cgagggaggg cagtgatgtt caagcgacca accgtgcatt acggccggac gccggagccg
     6301 gtcacgccgt atcagagcgc ggcgcaggcc tgggatgagc gcatcggctc ggcgcgggtc
     6361 caggccaaaa actggcggtt gatggcgttc ggatgcctgt tgctgtcggg agggcttgca
     6421 gcagccctcg tctggcaatc cacgcaaggc accgtcacgc catgggtggt ggaggttgac
     6481 catctcggtc aagcccaggc ggtcgcgccg gcaagtccgt tctatcagcc gaccgatccg
     6541 caaatcgcgt ttcacctcgc gcgcttcatc gaagatgtgc ggggcctgcc ggccgatgcc
     6601 gtcgtgctca ggcaggattg gctccgtgcc tacgaattca cgactgatcg cggagctgcg
     6661 gcgctgaacg actacgcgcg taccaacgat ccctttgcca agctgggaaa tatgcaggtt
     6721 gcggtcgaaa tctccagcgt catccgtgcc tcgccggaaa gctttcgggt cgcatggact
     6781 gaacgccgct acgacaacgg ccagctcgcc gcgaccgagc gctggaccgc gatcctttcc
     6841 gtcgtcatcg aaaccccacg tgatgccgac cgcctgcgca agaacccact gggcgtttac
     6901 gtcaacgcta tcaattggtc gaaggagctc ggttcgtgag agaggctata tcgataccgg
     6961 ccagcaatgg agcgcgcgta cgccttgcat tgtttttctt gctcgctctc tccggctgca
     7021 cgaccttcaa accaccgcag atcagctacg atgacgacgt accgccgtct cccgatctac
     7081 cagcgccggc ggacgatcat gcgcgaccat tgcatgtccc tccagcctgg actccggcac
     7141 gtggcggcaa caagggtgaa gcggaggcaa aggacccgac cgatcgtatc gagattgcca
     7201 acgatgtcgc gcgggtccag ccccggcgcg cagggtattt caacgccgtc cagatctttc
     7261 cctatagccc tggcgcgctc tatcaaatct atgcctcgcc agggcaaatc acggatatcg
     7321 agctcgaacc gggcgaacag ctgactggct ccgggccgct cgcggccggc gataccgtgc
     7381 gctggatcgt tgtcgacacc gaaagcggca acggcgatac taggcgcgtc cacgtcatgg
     7441 ccaaaccgac gcgtcccgct atcgagacga accttgtcgt caataccgat cgacgcacct
     7501 acctgattga actacgctcc cgcgaaaagg cgtacatgcc gtcggtcgct tggttctatc
     7561 cggaaaatcg gagcggacat tcaaagccct tgcctccgac gcccttcatc ccagaagttg
     7621 agcagcgccg atatcgctat atgatcgagg gcgacaaccc gccgtggcga ccggtcaatg
     7681 cgtacgatga cggccgcaaa gtctatatcg aattctcgcc cgggatcggg cagcgcgaaa
     7741 tgccgccgct cttcgtcatc ggccaggacg gcaagcctga actcgtcaac tatcgcgtct
     7801 acgggaacgt cctgattgcc gaccgtctgt tcgccgcggc cgagctgcgc cttggcgcag
     7861 acaagcagca gagggtccga atcgtcagaa acaacgggag gcgatcgtga caggcaatgc
     7921 gccaaacgag agcctcggtc ctgcggcggg cccaacgcct tcgtctgaag agccggcgca
     7981 tgccttgcgt ttgcgtgcgg agcgtccacg gatcacgcgg ctttcccgaa aagtccttgc
     8041 gggtggcacc gctctagctt tgctgctcat ctcaggcgcg gtgctgtggg cgttgaggag
     8101 cagccatcca cacaatccag ctccggacga actctacagc acggatcatc acaacattgc
     8161 agatggcttg acgacgttgc cacacgacta tgctggcatt gcccgcaacg tcccacagct
     8221 cgggccgcca ttgcctggcg acttgggacg tcccattgtc acggcggaag gccaatctac
     8281 accggtcggc cttgatgctg aacaacaacg tgccgaccag gaaacggagg cggctcggac
     8341 cagcaaatta tttgcgccga ccacagcgct cgtcactccg ccacgtgctc catcccagga
     8401 aacggctacc aaaacggcgt catcttctga cgaaaccttc gcgcagaacg ggcaggatcg
     8461 caaactgctg ttcgtcaacg cccccgtcga tcggcggacc acggcgcccg acaggctttc
     8521 acgtcccgcg tcgccctttg tcatccaagc gggaacgatc attcctgcgg ccctcattac
     8581 cgggatttgt tctgatcttc ccggccaaat cagcgcccaa gttacagaag cgatttttga
     8641 ttctccgacc ggccgcttca agctagttcc gcaaggagcg cgactgatcg gcatttatga
     8701 tagccaggtc gcatttggtc aatctcgggt gcttttggtt tggacgcggc tcatcatgcc
     8761 caatggtcgc tcgatcgttc tggaacggca acaaggggct gatgctggcg gctactccgg
     8821 cctagaggat gaggtcgaca atcattggaa agagttgctt ggggccgcag cgctatcgac
     8881 gttgcttgga gtaggttcag agcttgggtc cggcgccgac aacggaaaca acacagccat
     8941 tctccaagcg ctt
//
LOCUS       DATLJT010000223         6658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3686720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000223 DATLJT010000000
VERSION     DATLJT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3686720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VM910_05100"
     CDS             complement(<1..296)
                     /locus_tag="VM910_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61956.1"
                     /translation="MRIQNAAVKPFDVTRLEDEVRDAVQQRQPPVQAVDYTPRTTAPP
                     VPEYVSHREDVTEIGKLSAEAIVKEYEATAKEIEAMGEFVKEMVQRCEQLTASSA"
     gene            complement(982..3042)
                     /locus_tag="VM910_05105"
     CDS             complement(982..3042)
                     /locus_tag="VM910_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI61957.1"
                     /translation="MNSAIESSSTNPAADPGGNLRAGRGSVLSRLLVVTVVAVCVVHL
                     VGALVVAGLAAQSAERQFEDHVSTVMESRAKLMATPLWKMQYENLGSILAELAGDPAI
                     LSAKVVDDTGTVVARTGKVDASTWGGVRSAQIVYHDGNIMSVAGRFEMTVSRASINVA
                     YWLALKQALLIAALATGAIAMGLWFATQRYIGRPLSLISAAIDRSRLDGKRYRVEWQS
                     EDEFGALAHAFNAMQHAADRSEYALRAANQRLDFLAMHGELTGLPNRRSFEDRLTVAA
                     NANLPKPATLAVHFIDLDDFKAINDTLGHAAGDALLRHVAACLRAVVRDDNFAARFGG
                     DEFVVLQAGVAGEEDARHFAESLLAAIGHPLALQDHTLQANASIGVAVLDDAQSNVEK
                     LLSFADIALYEAKRSSRGTISFLTSARHDAHSRRRELERDIRAMTGWSEFVLFFQPQV
                     DMESGCPVGLEALVRWQHPMHGLLGPMEFLPLIEGMGLSSEFGAHIVREGCRAARLLQ
                     DRGHHDLRVAVNLAPAQLIDRYLTQLFVQQISTLQLPAGSLEAEITEGALIRDPANAK
                     KVLSGLRRLGVSVALDDFGTGYSSLAYLRRFPIDRIKLDRSFVRELPETRETAAIVRA
                     IRDLAGALGVELIAEGIEREAEAHFLLAENVCIAQGYLYCKPRPLDDICAWLVEQRLA
                     SQAA"
     gene            complement(3065..4204)
                     /locus_tag="VM910_05110"
     CDS             complement(3065..4204)
                     /locus_tag="VM910_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61958.1"
                     /translation="MKKLCLLVLAAFLFPAQAPADPILKRPQIVFLNPGKSGEVFWDL
                     VSDTMRAAASQLHVDVEIIHSERNRRMLNDLGMAIVGRPAHPDYLILVNEESAATPVI
                     EAANARGIKVFLLSNAFTGEEAKKYGAPREVLQNWIGSLTPDMPAASRRMASALIETA
                     RRANHVSADGKLHLLALGGDELTPNSITRNDGFTAFVAGKSDVVVDRFLYANWNAAEA
                     ETLTARYLDWAEHSGIRPAGDWAANDPMALGAIEAIAKKGLVPGKDMAVVGLNWSPEA
                     LDAIKAGKLLLTDGGHFFGGAWSIVMLRDHADGCDFAGDGAAKTFPLAAIDQSNLAQL
                     GGIISERRFEAIRFEQFLASRRGRCGAYDFSLDALVRAARAPMQN"
     gene            complement(4358..4906)
                     /locus_tag="VM910_05115"
     CDS             complement(4358..4906)
                     /locus_tag="VM910_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61959.1"
                     /translation="MISHLARLIALSGAVLFIIGMPHRIAVAAAAGCGTVAPPLLDRC
                     LRAPVTGGRPMLAQAAPQDNELSAEQRARLKAEIARVPSDLRGRFDVRYRAWKYSWRR
                     KPILFNTRTARGTAEFRALVALGPGILPLLVDRLVRRDEYAALQAYDALQDKPDLRVD
                     PQNYPSEQQRALETARRWLART"
     gene            4957..5097
                     /locus_tag="VM910_05120"
     CDS             4957..5097
                     /locus_tag="VM910_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61960.1"
                     /translation="MTSSFATLALVIDPIGLAPAFIAITQGFDATQFVVDGVKGAFHW
                     MA"
     gene            complement(5114..5992)
                     /locus_tag="VM910_05125"
     CDS             complement(5114..5992)
                     /locus_tag="VM910_05125"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61961.1"
                     /translation="MPKFKIEAHFRLQEWVAEEKGYFRDEGLDYEFKEFIRSSGGAHH
                     ATTGHSGAFQSIEKGRESNVTCACHWTVNVAASRGHTRLFADAYSVAPAGIFVHANSA
                     ITTPADLAGVPISVGFQSGSHYATIQALESYLAKDQINLSFDEGMLFGRMEAFFEGRA
                     QAASLFGGPYYFAEQLGYRKIIDTTFMIAAMVNGDPDMEDVKRYFRALRRAQRDIDLR
                     PERYTHYYKNEFPERFHAAMDTRRWGPGERIVFEPYTREVFSQSFDWIARHEIFEAGS
                     MGAGDYDKAVVSVGLA"
     gene            6222..>6658
                     /locus_tag="VM910_05130"
     CDS             6222..>6658
                     /locus_tag="VM910_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HVI61962.1"
                     /translation="MHLPYHDRYAYSPIIDRPDYSWPDGKRLAVYLGLNIEHFAFGAG
                     EGHLLTVPGVAPDARTFAWRDYGNRVGVWRCLELFDELNLPAAHLINTTVFDYAPRIV
                     DALKARGDEFIGHGRTNAERHSHMWESDEKRFLQDVRDAIERAS"
BASE COUNT         1237 a         2107 c         2079 g         1235 t
ORIGIN      
        1 gccgaggacg cggtgagctg ctcgcagcgc tgcaccatct ctttcacgaa ctcgcccatt
       61 gcttcgatct ccttggcggt cgcctcgtat tccttgacga tcgcctcggc cgaaagcttg
      121 ccgatctccg ttacgtcttc gcgatggctg acatattccg ggaccggcgg tgccgtagtt
      181 cgtggcgtgt aatcgaccgc ctgaacgggc ggctgccttt gctggaccgc atcccggacc
      241 tcgtcttcga gacgggtgac atcaaatggt ttaaccgctg cattctgaat tctcataata
      301 ctcactcctt tggtaactct ctcacgtcgg gagctggata cttgcggcca gctcgtagca
      361 tcgtcgggcc atgctcctcg gaactcgagg acttaattcg acccgacaat ttcgaaatgt
      421 ctctttaccg gcagtcctgg atcacccctg gtgaaagcct ccgggccttg tattccatga
      481 acgcgccctt tcattgagac ctcccctgcg cagtgttgag ccatcgtccc aaaggcgctc
      541 tccacatgcg gaacactggt acaggccgac catgttccca ctccgggaat caaggatcga
      601 aagagttaga gtcagtcgag aaaacgccat acatctctga caacggcgta gcgcgttctc
      661 gactagcggt gcttcttgat ccgtcatgtc ctgctccgaa cgccacccca gcccggatta
      721 gggtcccgaa cgcagcgttg tggcgaagcg gggtcaaatt gcgtctttcc cacggagctt
      781 attgattagc ccattcggat tactccgcgt gcgaggattg ctgcgatcac atgttagcgc
      841 gggggcctcg ggcgaaaggc acaattgcaa tcgtccgatc agaaataccc cttttgatcg
      901 gaacgcaacc aactcacggc cccggcgccc aagaccgggg tcgcttttct ttgatcacct
      961 cgttcgaacg ctcgaagcgc gtcacgccgc ctgcgaggcc agccgctgct cgacaagcca
     1021 ggcgcagatg tcgtcgagcg ggcgcggctt gcaataaaga tagccctgcg caatgcagac
     1081 attctcggcg agcaggaagt gcgcctccgc ttcgcgctcg atgccctcgg cgatcagttc
     1141 gacgccgagc gcgcccgcaa gatcgcggat cgcgcgaacg atggcggcgg tctcacgcgt
     1201 ctccggcaat tcgcgcacga aggagcgatc gagcttgatg cggtcgatcg ggaaacggcg
     1261 cagataggct agcgacgaat agccggtgcc gaaatcatcg agcgcgaccg agacgccgag
     1321 gcgccgcagc ccggaaagca ccttcttggc gttggcggga tcgcggatca atgcgccttc
     1381 agtgatctcg gcctcgagcg agcctgccgg aagctgcaac gtcgatatct gctgaacgaa
     1441 gagctgtgtc agataccggt cgataagttg agccggcgca aggttgacgg cgacgcgcag
     1501 atcgtgatgg ccgcgatcct gcagcaagcg ggcggcgcgg cagccctcgc gcacgatatg
     1561 cgcaccgaac tcggaggaga gccccatccc ctcgatcaac ggcagaaatt ccatcggccc
     1621 gagcaggcca tgcatcgggt gctgccagcg caccagcgcc tcgagaccga ccggacagcc
     1681 ggactccatg tcgacctgcg gctggaagaa caacacgaac tcgctccacc cggtcatggc
     1741 gcggatatcg cgctcgagct cgcgccggcg gctatgggcg tcgtggcgcg cactggtcag
     1801 gaacgagatg gtgccgcggc tgctgcgctt ggcctcgtag agcgcgatgt cggcaaacga
     1861 cagcagtttc tcgacattgg attgcgcatc gtccagcacc gcgaccccga tcgaggcatt
     1921 ggcctgcagc gtgtgatctt gcagtgcaag cggatgaccg attgccgcca gcagcgactc
     1981 ggcgaagtgg cgggcatcct cctcgcccgc gacgccggcc tgcagcacta cgaattcgtc
     2041 gccgccgaag cgggcggcaa aattatcgtc gcggaccacg gcgcgcaggc aagcggcgac
     2101 gtggcgcaac agcgcatcgc ccgcggcatg gccgagcgta tcgttgattg ccttgaagtc
     2161 gtcgagatcg atgaaatgca ccgcgagcgt cgcgggcttc ggcaggttcg cgttcgccgc
     2221 gaccgtaagg cggtcctcga acgagcgacg gttgggcagg cccgtgagtt cgccatgcat
     2281 ggcgaggaaa tcgaggcgct ggttggcggc acgcagcgcg tattcgctgc ggtcggcggc
     2341 gtgctgcatg gcattgaagg catgcgccag tgcgccgaat tcgtcctcgc tctgccactc
     2401 tacccggtag cgcttgccgt cgagccggct acggtcgatc gcggccgaga tcaggctcaa
     2461 cggccggccg atatagcgct gcgtggcaaa ccagaggccc atggcgatcg cgccggtcgc
     2521 cagcgccgcg atcaggagcg cctgcttcag cgcgagccaa tacgcgacgt tgatcgatgc
     2581 ccggctgacc gtcatctcga agcggcccgc cacgctcatg atgttgccgt cgtggtagac
     2641 gatttgcgcg gaacgcaccc ctccccaggt ggaggcatca accttgcccg tcctggccac
     2701 gaccgtaccg gtatcgtcga ccaccttggc agataggatg gcgggatcgc cggcaagctc
     2761 ggcgagaatc gagccgagat tttcgtactg catcttccag agcggcgtcg ccatcagctt
     2821 ggcgcgcgat tccatgacgg tggacacgtg gtcctcgaac tgccgctccg cagattgagc
     2881 ggcaagccct gcaacgacca gcgcgcccac cagatgcacg acgcagaccg caacgaccgt
     2941 caccaccaga agccgcgaca gtacggagcc gcggcccgcg cgaaggttcc cgccgggatc
     3001 ggcagccgga tttgtcgaag acgactcgat cgcggagttc atggtacgcg ctgtcgctga
     3061 tcggtcagtt ttgcatgggg gcgcgggctg cccgcacaag cgcgtcgagc gagaaatcgt
     3121 aagcgccgca ccggccgcgg cgtgacgcaa ggaactgttc gaagcggatc gcttcgaagc
     3181 gccgctccga gatgatgccg ccaagctggg caaggttcga ctggtcgatg gcggcgagcg
     3241 ggaaagtctt tgctgcaccg tcgccggcga agtcgcagcc gtcggcatgg tcgcgcagca
     3301 tgacgatcga ccaggcgccg ccgaagaagt gaccgccgtc ggtcaacagc agcttgcccg
     3361 ccttgatggc gtcaagcgct tccggcgacc agttgaggcc gaccacggcc atatccttgc
     3421 cgggcacgag gccttttttg gcgatcgcct cgatcgcgcc gagcgccatc gggtcgttcg
     3481 ccgcccagtc gccggccggg cggatgccgg aatgttcggc ccaatcgaga tagcgggcgg
     3541 tgagcgtctc ggcctcggca gcgttccagt tcgcatacag gaagcgatcg accacgacgt
     3601 cgctcttgcc ggcgacaaag gcggtaaatc cgtcattgcg cgtgatcgaa ttcggcgtca
     3661 gctcgtcgcc cccgagcgca agcagatgca gcttgccatc ggcgctgacg tggtttgcgc
     3721 gccttgcagt ctcgatcaac gcactcgcca tgcgccggga ggctgccggc atgtccggcg
     3781 tgaggctgcc gatccagttt tgcaacactt cgcgcggcgc gccgtatttt ttggcttcct
     3841 cgccggtgaa ggcgttggaa agcaggaaga ccttgatgcc cctggcattc gccgcctcga
     3901 tcaccggcgt tgccgcggat tcctcgttca ccaggatcag atagtcggga tgcgcgggac
     3961 gaccgacgat cgccatgccg agatcgttca gcatccgccg gttgcgttcg gaatggatga
     4021 tctcgacgtc gacgtggagc tggctcgccg ccgcgcgcat ggtgtcactg accaggtccc
     4081 agaacacctc gccggatttg ccgggattga gaaagacaat ctgcggccgt ttcaggatag
     4141 gatcggcggg cgcctgcgcg ggaaacagga atgcggcgag taccagcagg cagagcttct
     4201 tcacgaccgt cccccaatga ttgccgagac ttagccgatg aagttctaag attatatgga
     4261 agcttagggt taagcggtcg ttgcaattga gtagggtcgg gagtggaaaa tcgaccatcg
     4321 ctcgctagaa caccgcgcaa gaccaccccg ctccggttta cgtccttgca agccagcgcc
     4381 gcgccgtctc cagcgcgcgc tgctgttcgc tcggatagtt ctgtggatcg acgcgcaagt
     4441 ccggcttgtc ctgcaaggcg tcataggctt gcagcgcggc gtattcatcc cgccgcacca
     4501 gcctgtcgac gagaagagga aggatgcccg gaccgagtgc gacgagcgca cggaattcgg
     4561 cggtgccgcg agccgttctg gtgttgaaca atatcggttt tcgccgccag ctatatttcc
     4621 aggcgcggta tcgcacatcg aagcgcccgc gcaggtcgga tggcacacgc gcaatctccg
     4681 ccttgagacg cgcgcgctgc tccgccgaga gctcgttatc ctgcggtgcc gcctgagcca
     4741 acatcggtcg tccgccggtg accggggcac gcagacaacg gtccagcaaa ggcggagcga
     4801 cggtcccgca gccggccgct gccgcaaccg cgatgcgatg cggcattccg atgatgaaca
     4861 ggacggcgcc tgacagcgcg atcagtctgg caagatgcga tatcatcggc tattcgaacc
     4921 tgttggcggg acgattcgtt ttcatatcct aattacgtga cctcgtcctt cgcgacattg
     4981 gcgctggtga tcgacccgat cggactcgcg cccgccttca tcgccatcac gcaaggcttc
     5041 gatgccacgc aattcgtcgt ggacggggtc aaaggcgcgt ttcattggat ggcatgaaac
     5101 ggcgcgcgcc ccctcaggca aggccgaccg atacgaccgc cttgtcgtag tcgccggcgc
     5161 ccatgctgcc cgcctcgaag atctcgtgcc tggcgatcca gtcgaaggac tgggagaaga
     5221 cttcgcgggt atagggttcg aacacgatcc gctcgcccgg tccccagcga cgcgtatcca
     5281 tcgcggcgtg gaagcgctcg ggaaattcat ttttgtaata gtgggtataa cgctcgggcc
     5341 gcaggtcgat atcgcgctgc gcccggcgca gcgcacggaa atagcgtttg acgtcctcca
     5401 tgtcgggatc gccgttgacc atcgcggcga tcatgaacgt ggtgtcgatg atcttgcgat
     5461 agccgagctg ctcggcgaaa taatagggac cgccgaacag cgaggcggcc tgtgccctgc
     5521 cctcgaagaa ggcttccatc cggccgaaca gcatcccctc gtcaaacgac aggttgatct
     5581 ggtcttttgc gagatagctc tcgagcgcct ggatagtcgc gtagtggctg cccgactgga
     5641 atccaaccga aatgggcacg ccggcaaggt ccgccggcgt cgtgatcgcg gaattggcgt
     5701 gcacgaagat gccggccggc gccaccgaat aagcgtccgc aaacagcctg gtgtggccgc
     5761 gcgatgcagc gacgttcacc gtccaatgac acgcgcaggt gacgttggat tcgcgtccct
     5821 tttcgatgct ctggaacgcc ccgctgtgac cggtggtggc gtggtgggcg ccgccgctcg
     5881 agcggatgaa ctccttgaat tcataatcga ggccttcgtc gcgaaaatag cccttctctt
     5941 ccgccaccca ttcctgcaac cggaaatgcg cttcgatctt gaacttcggc atggcgtcgg
     6001 ctcccgagcg atctttgttc tggtccccgc tttgctgcga gcttatcgcg cagcacaagc
     6061 gggcgcgagg gcgaaatcat gtttctcatg gcgcggtccg tatgcgcggc atggccgtgc
     6121 tgcgtcgcca cagccgcggt tgtgctcatc tcaggcctgg tgtcagatgg acgacaacgc
     6181 cgttcggtca cgccaggact cgtttcggag ggaataccga gatgcacctc ccctatcacg
     6241 atcgttatgc ttactcgccg atcattgacc gcccggacta ctcctggccg gacggaaagc
     6301 ggctcgccgt ctatctcggc ctcaacatcg aacatttcgc gttcggtgcg ggcgaaggtc
     6361 atctcttgac cgtgcccgga gttgctccgg acgcccggac gttcgcctgg agggattacg
     6421 gcaacagggt cggcgtttgg cgttgcctcg aactcttcga tgaactcaac ctgccggcgg
     6481 cccatctcat caacacgacg gtcttcgact atgcgccccg catcgtcgat gcactcaagg
     6541 cgcgcggcga tgaattcatc gggcatggcc gcaccaacgc cgagcgccac agccacatgt
     6601 gggaatccga cgagaaaaga ttcctgcagg acgtccgcga tgcgatcgaa cgcgcgtc
//
LOCUS       DATLJT010000224         4248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_930073,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000224 DATLJT010000000
VERSION     DATLJT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930073"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..876
                     /gene="fghA"
                     /locus_tag="VM910_05135"
     CDS             31..876
                     /gene="fghA"
                     /locus_tag="VM910_05135"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HVI61963.1"
                     /translation="MTIQTVSLNKSYGGMQGVYKHASRETKTDMTFSVYVPSHPDGGK
                     LPVVYYLSGLTCTHANVTEKGEYRRACAELGLVFVAPDTSPRGADIPGDPNNAYDFGL
                     GAGFYVDATEAPFARNYRMWSYVTEELPRTIAEHFPVDPTRQSVFGHSMGGHGALTVV
                     LRYPDCYRAASAFAPIVAPSQVPWGIKALSGYLGSDRQAWRKHDAVALIGDGARFRDF
                     LVDYGDADPFLNEQLRPELLKAACEKARIPLTLRRQEGYDHSYYFISTFMSDHLGWHA
                     ERLKG"
     gene            complement(882..1370)
                     /locus_tag="VM910_05140"
     CDS             complement(882..1370)
                     /locus_tag="VM910_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HVI61964.1"
                     /translation="MLTSAAQVGAAEPPKLAVFDLEMIDTSLQGEVYGPRSEEHDRLM
                     RVGDQLRKELGESGEFRIIDIGPVDAAAHQSNLQACGGCDVKFAQRLDADLFITGVVQ
                     KVSNLILSINVYLRDAHTGRLVAVANADMRGNTDESWSRAMRYIIRNRLLAPNYGMPQ
                     QR"
     gene            1518..2699
                     /locus_tag="VM910_05145"
     CDS             1518..2699
                     /locus_tag="VM910_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61965.1"
                     /translation="MIRWLIGAIGASVVAAHALAAEPIEIGIGYLGQANVKSTLSLVL
                     QPAENDGVAGAHLAIEDNNTTGKFLNQHFTLHEVRLKEGEDVAKAASSLADHDNFIIV
                     DLPADQLLKAADALRDRGTVLLNAGAIDDRLREEDCRANVIHVAPTRSMLADALAQYL
                     VWKQWKRWLLVVGSHDADKLYADALRRAAARFGAKIVQERTFEDTGGARRTDSGVTLI
                     QRQIPVFTQQAPAYDVLVAADESEVFAAYLPYRAWDPRPVAGSAGLVPTSWDAAQDQW
                     GATQMQNRFMKLNARRMTAKDMQAWTAARVIGEATSRTNSGDAKTVLDFIKGKDFSVA
                     AFKGQRLTLRDWNLQLRQPILLVDGRMVVSVSPQEGFLHQVSELDTLGLDRPETKCKL
                     Q"
     gene            2717..3688
                     /locus_tag="VM910_05150"
     CDS             2717..3688
                     /locus_tag="VM910_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HVI61966.1"
                     /translation="MSHHRLLCLTVLLLAWTNPAWSFVAYVSNEKSNTVSVIDTDTWT
                     VTATIKVGQRPRGIEFTRDGKFVMVAVGDDDTIQVIDTKTQKVVDTLPSGPDPELFTQ
                     DAAGKILYVANENDNTVTVIDLQKRSRLGDIQVGVEPEGMAVSPDGKILINTSETTNM
                     AHFIDTTARQVVANVLVDSRPRFAEFKRDGSELWVSSEIGGTVSIIDPAKHAVTGKIS
                     FNIPGLRSEAIQPVGIAMTKDGKTAFVALGPANRIAVVDATSHAVTKYLLVGQRVWHM
                     AFTPDEKYLLTTNGVSNDVSVIDVAAQKVIKTIQVGELPWGVTIAQP"
     gene            3802..>4248
                     /locus_tag="VM910_05155"
     CDS             3802..>4248
                     /locus_tag="VM910_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI61967.1"
                     /translation="MRRALTDVSFTVAPASFTALLGLNGAGKSTLFSIITRLFGLQAG
                     RIEIFGHDIGREPGEALRLLGVVFQPRTLDLDLSVMQNLLYHAALHGIARREARTRGD
                     EVLARLGLADRAGSKVRDLSGGQMRRLEIARALLHRPRLLLLDEATV"
BASE COUNT          824 a         1367 c         1311 g          746 t
ORIGIN      
        1 cgccatcccg ttcgcacagg atttcaggac atgacgatcc agaccgtttc tctcaacaag
       61 tcgtatggcg gcatgcaggg cgtttacaag cacgcgagcc gggagaccaa aaccgacatg
      121 acgttttcgg tctatgtgcc ctcgcacccc gacggcggca aactgccagt ggtctattat
      181 ctgtcgggac tgacatgtac ccacgccaac gtcacggaga agggcgaata ccgccgcgcc
      241 tgcgcggagc tcggtctcgt cttcgtcgcg cccgacacaa gcccgcgcgg cgccgacata
      301 ccaggcgatc caaacaatgc ctacgatttc ggactcggcg ccggcttcta tgtcgatgcc
      361 acggaagcgc cgttcgcgcg caactatcgc atgtggagct atgtcacgga agaattgccg
      421 cgcacgatcg ccgagcattt cccggtcgat cccacgcgac aatctgtctt cggccactcc
      481 atgggcggac acggtgcact gacggtcgtg ctgcgctatc ccgactgcta ccgcgcagcg
      541 agcgcctttg cgccgatcgt cgcgccgtca caggtgccct ggggcatcaa ggccctcagc
      601 ggctatctcg gcagcgatag gcaggcgtgg cgcaagcacg atgcagtcgc gttgatcggg
      661 gacggcgcca ggtttcgcga ctttctggtg gactacggcg acgccgaccc gttcctgaac
      721 gagcaacttc gcccggaatt gttgaaggcg gcttgcgaga aggcaaggat tcccctcacc
      781 ctgcgccgcc aagagggtta cgaccacagc tactacttca tctcgacctt catgagcgat
      841 catctaggct ggcacgccga gcgcctgaag ggatgaagcg cctatcgttg ctgcggcatg
      901 ccgtaattgg gcgcaagcag gcgattgcgg atgatgtagc gcatagcccg cgaccaggac
      961 tcgtcggtat tgccgcgcat gtcggcgttc gccaccgcta cgagccggcc cgtatgtgcg
     1021 tcgcgcaaat aaacgttgat gctgaggatg aggtttgaga ccttctggac cacgcccgtg
     1081 atgaagagat cggcatcgag ccgttgcgcg aacttcacgt cacaaccgcc gcacgcctgc
     1141 aaattgctct gatgcgccgc agcatcgacc ggcccaatat cgatgatccg gaactcgccg
     1201 gactcgccaa gttccttgcg tagctgatcg ccgacccgca tcaaccggtc atgctcctcg
     1261 ctgcgcggcc catagacctc gccctgcaga ctcgtgtcga tcatctcgag atcgaacact
     1321 gcaagcttgg gcggttcggc ggcgccgacc tgcgcggcac tcgtcagcat caatatgaaa
     1381 aaggcaatca gcgctctcac gatcgtgacc caaaaatggc gcaacgacaa aagtggggtt
     1441 gcaagctaag acaattcggt catgtttcaa gacgggttct agcgttcttc gatcgggaga
     1501 accaccaggg aggagtgatg atccgttggc tgatcggcgc aattggcgcg agtgtcgtgg
     1561 ccgcccacgc gcttgcggcc gagccgatcg agatcggcat cggctatctc ggccaggcca
     1621 acgtcaaatc tacgttgtcg ctcgtcctgc aacccgccga gaatgatggc gtggccggcg
     1681 cgcaccttgc gatcgaggac aacaacacga ccggcaagtt tctcaaccag cactttacgc
     1741 tgcacgaggt gcgcctcaag gagggggaag acgtggcaaa ggcggcgtcc tcgctcgccg
     1801 atcatgacaa tttcattatc gtcgaccttc ccgcggacca gttgctgaag gcggccgatg
     1861 cgcttcgcga tcgcggcacc gtgctgctca acgcgggcgc gatcgacgac cggctgcgcg
     1921 aagaggattg ccgcgccaat gtcattcatg tcgccccgac ccgctcgatg ctcgcggacg
     1981 cgcttgccca atacctcgtg tggaagcagt ggaaacgctg gctgctcgtg gtcggctcgc
     2041 acgatgcgga caagctttat gcggacgcgt tgcgccgcgc ggcggcgcgg ttcggtgcga
     2101 agatcgtgca ggagcgcacc ttcgaggaca ccggcggcgc ccgccgcacc gacagcggcg
     2161 tcacgttgat ccagcgccag attcccgtgt tcacccagca ggcgccggcc tatgacgtgc
     2221 tggttgccgc cgacgagagc gaggtctttg ccgcctattt gccgtaccgc gcctgggatc
     2281 cgcggcctgt ggcggggtcg gcaggcctcg taccgacgag ctgggacgcc gcacaggatc
     2341 agtggggcgc gacgcagatg cagaaccgct tcatgaagct gaacgcacga cgcatgacgg
     2401 caaaggacat gcaagcctgg accgcggcgc gcgtgatcgg cgaagcaaca tcgcgcacca
     2461 attcgggcga cgcgaagacg gtcctcgatt tcatcaaggg gaaagatttt tccgtcgcgg
     2521 ccttcaaggg ccagcgcctg acgttgcggg actggaacct gcagctccgc caacctatcc
     2581 tgctggtcga cggacgcatg gtggtctcgg tgtcgccgca ggaaggtttt ctacatcagg
     2641 tctcggagct cgatacgctg ggcctcgacc gccccgagac caaatgcaag ctgcagtgag
     2701 ggcggagacg tcgcgcatgt cgcatcatcg cctgctttgc ctgacggtgc tgttgctggc
     2761 ctggaccaat ccggcctggt cgttcgtcgc ctacgtgtcg aacgagaaga gtaacacggt
     2821 gtcggtgatc gacaccgaca cctggaccgt gacggcgacg atcaaggtcg gccagcggcc
     2881 gcgcggcatc gagttcacac gagacggcaa gttcgtgatg gtcgcggtcg gcgacgacga
     2941 caccatccag gtgatcgaca ccaaaacgca gaaggtcgtc gacaccctcc cctccggtcc
     3001 cgatcccgaa ctgttcaccc aggacgctgc cggcaagatc ctgtacgttg ccaacgagaa
     3061 cgacaacacg gtgaccgtca tcgatctgca gaagcgcagc cggcttggcg acattcaggt
     3121 cggcgtcgag cccgagggca tggccgtcag ccctgacggc aagatcctga tcaacacctc
     3181 agagacgaca aacatggcgc atttcatcga caccacggcg cgtcaggtgg tcgccaacgt
     3241 actggtcgat tcgcggccgc gctttgccga gttcaagcgc gatggctccg aattatgggt
     3301 atcttcggag atcggcggca cggtctcgat catcgatccg gcgaagcacg cggtgaccgg
     3361 gaagatcagc ttcaatattc ccggcctccg cagcgaagcg atccagcccg tcggcatcgc
     3421 catgaccaaa gacggcaaga cggctttcgt tgcgctcggg cctgccaatc gcatcgccgt
     3481 cgtggatgcc acaagccacg ccgtcaccaa atatcttctc gtcggccagc gggtgtggca
     3541 catggcgttc acgccggacg aaaaatacct gctgacgacc aacggcgtct ccaacgatgt
     3601 ctcggtgatc gatgtcgccg cgcaaaaggt catcaagacg atccaggtcg gcgagctccc
     3661 ctggggcgtg acgattgcac agccatgacg accaccgaca cagaccgccc cgtcactcaa
     3721 gccggctcgg atatcccggc ccggcacgat gtcggcgcag agcccgcatt gtcgatcacg
     3781 ggggtgagcc attcctacgg catgcggcgt gctctcaccg acgtcagttt caccgtggcg
     3841 ccggcaagtt tcacggcttt gctaggcctc aacggcgcgg gaaagagcac gctgttctcg
     3901 atcatcacac ggctgttcgg actccaggcc ggccgcatcg aaattttcgg ccacgacatc
     3961 ggccgcgaac cgggcgaggc gttgcggctc ctgggcgtgg tgttccagcc gcggacgctc
     4021 gacctcgacc tctcggtgat gcagaacctg ctctaccacg cagccctgca cggcattgcg
     4081 aggcgcgagg cgcgcacgag aggcgacgag gtgcttgcac ggctcggcct tgccgatcgc
     4141 gccggcagca aggtgcgcga cctctcgggc ggccagatgc ggcggctcga aatcgcacgc
     4201 gcgttgctgc atcggccgcg acttctgctg ctcgacgagg cgaccgtc
//
LOCUS       DATLJT010000225         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2768620,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000225 DATLJT010000000
VERSION     DATLJT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768620"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VM910_05160"
     CDS             <1..987
                     /locus_tag="VM910_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HVI61968.1"
                     /translation="PHRHSSERKARWEFVSSPLGVIDLLAVLPFWFAFVLPGDLRVLL
                     VFRMVRFLKLARYSPAMRSLLDALYSERRALFGCFVILLGATLLAASIMHVIEGHAQP
                     DKFGTIPDAMWWAIVTLGTIGYGDVVPVTVLGRIVASVTIFVGLIMVALPIGIVATAF
                     ADEIHRRDFVVTWGMVARVPLFADLQATDIADIVRLLRAQRVEAGDVIAKRGEPAHSM
                     YFVAAGEVEIELKHERVRLGAGHFFGEVAALRRARRSATVIALTRTSLLVLDAHDLHV
                     LMEREPRIAQRIREVARTRLGEDAITHKGDLVVEELEEAETIQRSPGSHSRS"
     gene            complement(996..1868)
                     /locus_tag="VM910_05165"
     CDS             complement(996..1868)
                     /locus_tag="VM910_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI61969.1"
                     /translation="MPSGDVTRSIGVVIDQGKQEDPLSFAALMVHVDVEHDSEQRVEL
                     AIGLADRFQAAVIGVAGCALWPAFMAGDVELSKANQYDFQRMMVRFEQRGKKFRAQGH
                     SLRQIEWRSALESPGELLLREARAADLLVIGRRRSGDEADPGVIVLRAGRPVLLVPDT
                     AAALPLRRVVVAWKDTRECRRAVRDALPLLQQAKEVLLVEIGEDESQTQAKKSLADVA
                     AYLVRHRVIVAAEVWRRPRGQVAAELLQVVEDEKADLIVAGGYGHSRLGEWIFGGVTR
                     ELLTASPVCCMLSH"
     gene            complement(1906..2799)
                     /locus_tag="VM910_05170"
     CDS             complement(1906..2799)
                     /locus_tag="VM910_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI61970.1"
                     /translation="MATVTTAQSESSVGSFLDRGQLEAMSFTTMMVHLDVERDCEQRV
                     QLALALADRFQAALIGVAGLALRPAFAAGGVVVYCQPTEHECRAASARLDDIGKKFRE
                     QGQHLKEVEWRTALELPYEFVSRETRAADLIIVGARHTGGNMQDLADPGVILLRAGRP
                     VLVAPDTVAPLQLRRVVVAWKDTRECRRAVRDALPFLQRAKEVLLVGIGDGEAESNAK
                     KTLSDVAEYLLRHRVAAVHDVWRQARGPVAGELLRLVQDEKADLIVAGGYGHSRLGEW
                     LFGGVTHELLASSPVCCMLSH"
BASE COUNT          570 a          994 c          940 g          583 t
ORIGIN      
        1 ccgcatcggc attcgagcga acgcaaggcc cgctgggagt ttgtttcgag tccgctcggc
       61 gtgatcgacc ttctggcggt cctgccgttt tggtttgcat ttgtcttgcc aggcgatctg
      121 cgggtcctgc tggtgtttcg aatggtgcgg tttctcaagt tggcgcgcta ctcaccggcc
      181 atgcgctcgc tgctggacgc tctctacagt gagcgccgtg cgttgttcgg ctgcttcgtg
      241 attttactgg gtgcgacgct gctcgctgcc agcatcatgc acgtgatcga gggtcatgcg
      301 cagcccgaca agttcggcac gattcccgat gcgatgtggt gggccatcgt cacgctgggc
      361 acgattggct acggcgacgt cgttccggtg acggtgctgg gacgcatcgt cgcatcggtt
      421 acgatcttcg tcggattgat catggtcgcc ctgccgatcg gcatcgtcgc cacggccttc
      481 gccgacgaga tccatcgccg cgactttgtc gtcacctggg gcatggttgc gcgcgtgcct
      541 ttgttcgccg acctgcaggc caccgatatc gccgacatcg tgcggctctt gcgcgcgcag
      601 cgggtcgagg ctggagacgt catcgccaag cgcggcgagc ccgctcactc catgtatttc
      661 gtcgccgccg gagaagtgga gattgagctc aagcacgagc gcgttcggct cggcgccggt
      721 catttcttcg gcgaggtcgc ggcgctgcgg cgggcgcgca ggtccgcgac cgttatcgcg
      781 ctcacgcgga caagcctgct cgtgctcgat gcccacgatc tgcacgtgct catggagcgg
      841 gaaccgcgta tcgcgcagcg tatccgtgaa gtggctcgca cccggctcgg cgaagacgcg
      901 atcacgcaca aaggcgatct tgtagtcgaa gagctcgagg aagcggagac gatccaacgc
      961 tcgccgggta gccatagtcg ctcatgacgc gaagctcaat gcgacaacat gcaacaaact
     1021 gggcttgccg tcagaagttc gcgggtgacg ccgccgaaaa tccattcgcc caatcggctg
     1081 tgcccatagc cgccggcgac gattaagtct gctttctcat cctccacaac ctggaggagc
     1141 tcggcggcca cttgcccccg cggtcgccgc cagacttcgg ccgccacgat cacccggtgg
     1201 cggacgagat acgcggccac gtcggccaga ctcttcttgg cctgcgtctg cgattcgtcc
     1261 tcgccgattt cgaccagcag aacctctttg gcctgctgca agagcgggag cgcgtcccgc
     1321 acggcgcggc ggcattcacg agtatccttc caagcgacga ccacgcggcg aagcggcagt
     1381 gcagcggcgg tgtcgggcac cagcagaacg gggcggccgg cccgcagcac gataaccccg
     1441 gggtcggcct cgtccccgct gcgcctgcga ccgatgacga ggagatccgc cgcccgggct
     1501 tcccgcaaga gaagctcgcc gggggattca agggcgctgc gccattcgat ctgccgcagc
     1561 gagtggccct gggcgcgaaa cttcttgccc ctctgctcga agcgcaccat catcctttga
     1621 aagtcgtatt ggttggcttt gctcagctcg acgtctccag ccatgaacgc cggccacagc
     1681 gcgcaacccg caaccccgat aacggcggct tggaagcggt cggccaggcc gatcgcgagt
     1741 tcaactcttt gttccgaatc gtgctcgacg tcgacgtgga ccatcagtgc tgcaaaggac
     1801 aacggatctt cctgtttgcc ttgatcgatg acgactccga tcgaccttgt cacatctccg
     1861 ctcggcatca gcgttctccc acatgcaacc aaaacgaacg aatgctcagt gcgacaacat
     1921 gcaacagacc ggactcgatg caagaagctc atgcgtgacg ccgccgaaga gccattcccc
     1981 caatcgactg tgcccgtagc cgccggcaac gatcaaatct gccttctcat cctgaacgag
     2041 acgcaggagt tcgccagcga ccggtccccg tgcttgccgc cagacgtcgt gcaccgcggc
     2101 gactcgatgg cggagaaggt actctgcgac gtcggaaaga gtttttttgg cattggattc
     2161 cgcttcgcca tcgccgattc cgacgagcag gacctctttg gcccgctgca aaaacggaag
     2221 tgcatcacgt acggcgcggc ggcactcacg cgtgtctttc cacgctacca ccacgcggcg
     2281 gagctgtagc ggggcgacgg tgtctggcgc cactaggacg ggccggcccg cccgtagcag
     2341 gatcacgccg ggatcggcaa ggtcctgcat gttaccccca gtatgccgtg ctccgacgat
     2401 gatgagatcc gccgctctgg tctcgcgcga cacgaactca tacggcaatt caagcgcggt
     2461 gcgccattca acctctttga gatgctggcc ttgttcgcgg aacttcttgc cgatgtcatc
     2521 gagacgtgcg ctcgccgcgc ggcactcatg ttccgtgggc tggcaataga cgacgacacc
     2581 ccccgcagcg aaagcaggcc gcagcgccaa gccagcgacg ccaatgagcg ctgcctgaaa
     2641 gcggtcggcc agagcaagag caagttgcac tcgctgttca cagtcgcgct cgacatcgag
     2701 gtggaccatc atcgttgtga acgacatcgc ttccagctgg ccgcgatcaa gaaacgaacc
     2761 gaccgaactt tccgactggg cggtggtcac cgtagccacg agcgctgctc caagccaatc
     2821 cctcttttga atggtgacac gctggaggag gctgaaagtt gacgtggatc aagaatggca
     2881 ccggccggca aagcgcgtgt aaaggaagcc ggccgcaatc ggttaatcga tcgaacctgt
     2941 gtccgataag aagctgttac tgctgctgaa tggattgcag ataatcgatg atcgcaccca
     3001 cgtcctccgg cgcgaagacg aactccggca tgtccggatg accggtagaa aggccctccg
     3061 ccaaggcctc ggcaaggcct tcgatct
//
LOCUS       DATLJT010000226         8815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_11212,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000226 DATLJT010000000
VERSION     DATLJT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11212"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VM910_05175"
     CDS             <1..664
                     /locus_tag="VM910_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI61971.1"
                     /translation="LNAIIGFSEIIKLDAIQAKERYPQYAEYIHDAGILLLNIINGLL
                     DLARIQAGRVNLDEQIVAVGELMQSAIRTIAPIAQKKSIYIDCELAQASTLICVDQTK
                     FRQIILNLLSNGVKFTEPKGSILISSTMDGRGELVISIKDTGIGIPPEHLERVLSPFE
                     QVADHLTKENEGTGLGLPIAGALIELHGGNLTLSSDLGIGTTVALRLPSERIHGVAPA
                     RS"
     gene            740..2359
                     /locus_tag="VM910_05180"
     CDS             740..2359
                     /locus_tag="VM910_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HVI61972.1"
                     /translation="MSGSASESINLRIGRVIAITPSHAIVLLERRDATGMSDPAMPLE
                     MGTLVKLHSRVSTVYGMITGLRVPLPSLEPSEKDLRLVELELVGEIQGADGTGFFRRG
                     VSAYPALDEPVYLASAIDLAKVYARPNTATAPVGTIHQDSIVPAYILTDELLGKHFSI
                     VGTTGSGKSCVVATILNAVIERNPNAHVMLLDPHNEYASAFKERAASLSPGGGLYLPY
                     WLFNFEELVEIVIGADHNPEQVKILGEAVLSAKQSYFAKVGLDKFGTVDTPAPYRMSD
                     ALRFLDTAVGSLNRPDSVVAYQTVKGRILALQNDARYEFVFGGRLTLRDELSDILSQL
                     FRIPVNGKPITILDLSGIPSEVLNVVVSVLCRLAFDFALWSETPVPITIICEEAHRYA
                     PRDKELGFEAAKRALFRIAKEGRKYGVSLCVVSQRPSDLAPGLLSECNTMFAFRMTSH
                     EDQDIVRSAVPGASHGLMNFLPALRNGEAIAVGEGVAMPMRICFAPLPEDRRPKSATA
                     SFTIAWSKDSDGSHIERTVERWRRGMRHGPG"
     gene            2477..4345
                     /gene="htpG"
                     /locus_tag="VM910_05185"
     CDS             2477..4345
                     /gene="htpG"
                     /locus_tag="VM910_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVI61973.1"
                     /translation="MTQETRSFQAEVSRLLDIVAHSLYSQKEIFLRELISNASDACDR
                     LRYAALTEPALAEGDTSYRVVLSPDKSARTLTVADNGIGMNRAELIENLGTIARSGTA
                     AFISQLSGDARKDMSLIGQFGVGFYSAFMVAKKVEVKSRKAGESEGWRWVSDGKGEFT
                     IEPLPEAARGATIVVHLPEGEDEYLEPARLSRIVSTYSDHIGLPIVLKDGGKEETINT
                     ASALWTRPKSEITQEQYNEFYHHVGHSFDVPWLTLHAKAEGVLEYTSLLFVPSAKPFD
                     LFDPERKSRVKLYVRRVFITDEGTNLLPAYLRFLRGVVDSEDLPLNISREMLQSNPMV
                     ARIRQQLTKRILTELGKKANELPEEYVKFWDNFGAVLKEGLYEDREHREELLTLTRFR
                     STARNGLVSLDEYVTAMRPGQEAIYTITGDSLDVIKQSPQLEGFRARGVEVLLMTDPI
                     DEFWVPAIGSFKDKSFKSATRGGVDLDKITPPEDRKDEKSEPPAKLASLIAIFKLALG
                     DAVKDVRSSDRLTDSAVCLIADEGDMDMHFERLLKQHRQLDTMSKRILELNPHHKLIE
                     RLAATVGEAGASDQLSEFAWLLLDQARIIEGEQLPDAAAFARRLSTVLERGLSAAA"
     gene            5179..6615
                     /locus_tag="VM910_05190"
     CDS             5179..6615
                     /locus_tag="VM910_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HVI61974.1"
                     /translation="MTSPVALIQQQSFAPLVKKVSPAVVNISVSQKADADAMVEEPDM
                     SPSPGFPNSPFDEFLRRFFEQQGPGGQRHLFPETPGGQARRIALGSGFIVDPSGYVVT
                     NSHVVGDATKVEVTLQDDSKYTAKIIGRDPKTDLAVLKINADKPLPYVKFGDSSAAQV
                     GDWVMAVGNPFGLGGTVTNGIISARGRDIHSGPFDDFLQIDAPINRGNSGGPTFNLEG
                     EVIGINTAIYSPSGGSVGIGFAVPSNVAKTIIAQLEEHGKVSRGWLGVQIQEVTPAIA
                     ASLGLHGEKGALVAVVTSDSPGAKAGIKQGDVILSFNGTDVSHLRDLPRMVAGTAPDT
                     VATMTVWRNGEKVELHTKVGELANERVVSATNSGEDEDQQPPKAEALGMHFAPLTAQL
                     RRELHVGKDAQGVVVSRVDSGSAAEDVGLSEGDILVAIDQQPVNNPKEAAEKLKEVAN
                     SSKKNALLLLNRHGVTQYVGVNLANNNG"
     gene            complement(6724..8121)
                     /locus_tag="VM910_05195"
     CDS             complement(6724..8121)
                     /locus_tag="VM910_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVI61975.1"
                     /translation="MPALAFHREAVDASLLAELRQLLGDRLSTSAAVCAQHGKDESYH
                     TPHAPDAVTFAHSTEEVAAIVRLCSNHKTPVIAFGTGTSLEGHVAALAGGICIDLSQM
                     NQILRINAEDLDAVVEAGVTRKQLNEHLRDTGLFFPIDPGADASLGGMAATRASGTNA
                     VRYGTMRENVLTLTVVLADGRVIRTARRARKSAAGYDLTRLFVGSEGTLGVITEVTVR
                     LYGIPEAISAAVCAFPTIEDAVNTVILTIQNGVPVARIELLDEAQMTAINKYSKLNHK
                     VAPTLFFEFHGSTAGVAEQAETVKAIADEYGGNDFRWATTSEERSKLWQARHDAYYAA
                     LALRPGSKGWATDVCVPISRLAECIKETKRDLASSPIPSALVGHVGDGNFHLVFMIDP
                     QRPDEIAEAGRLNDRMVARALAMEGTSTGEHGVGYGKMDFLVSEHGEALSVMRAIKKA
                     LDPANILNPGKIVRI"
     gene            8307..>8815
                     /locus_tag="VM910_05200"
     CDS             8307..>8815
                     /locus_tag="VM910_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVI61976.1"
                     /translation="MALAAVTLDDKYVLDQGRIYLTGTQALVRLPMMQRQRDVAAGLN
                     TGCFISGYRGSPLGGLDQALWSARRFLEKSHIHFQPAINEELGATAVWGSQQVNLFPG
                     SKYDGVFALWYAKGPGIDRSGDALKHGNAAGSAPYGGVLLLAGDDHTCKSSTLPHQSE
                     YAFMDAGIPV"
BASE COUNT         1839 a         2586 c         2626 g         1764 t
ORIGIN      
        1 gctcaacgcg atcatcggct tttcggagat catcaagcta gacgccattc aggcgaaaga
       61 gcgttatcca caatacgccg aatacattca tgatgccggc atactccttc tcaatatcat
      121 caatggtctt cttgatctcg cacggataca ggcgggaagg gtaaatctcg acgagcagat
      181 agtcgctgtt ggcgagttaa tgcaatcagc gattcgtact atagcaccga ttgctcaaaa
      241 gaaatctatc tacatcgatt gcgaactggc tcaggcatcg acgcttatct gtgtcgatca
      301 aactaaattt aggcagatta tccttaactt attgtcgaat ggtgttaagt ttaccgaacc
      361 aaaggggagt attttgatca gttcgacgat ggacggccgg ggagaattgg tgatctcgat
      421 taaagacacg ggtatcggta ttccacctga gcacttggaa cgggtgctca gccctttcga
      481 acaggtagca gatcacttga ccaaagagaa cgaaggaact ggactggggc tgccgatcgc
      541 cggggcgcta atcgagttgc acggcggcaa tctgaccttg agcagcgatc tcggcatagg
      601 aaccacagtt gcgctgaggt tgccctccga gcgaatccat ggcgtggcac ccgcaaggtc
      661 atgacgcaac cttccccacc tcaacaatga ttagtggttc cgcgagccct ccgtcgcatc
      721 ctaccgaaga gacgaaacta tgagcggcag cgcttccgaa agcatcaatc tgcggattgg
      781 ccgagtcatt gccataacgc cctctcatgc gatcgtactt ctcgaaaggc gggatgcgac
      841 gggtatgtcg gaccccgcta tgccgctcga gatggggact ttggtaaagt tacacagccg
      901 cgtgtcgacc gtttacggca tgatcaccgg ccttagagtc ccgttgccca gcctcgagcc
      961 ctccgagaaa gatttgcggc tcgtcgagct tgagctggtc ggcgagatcc agggtgccga
     1021 cggcacaggc ttttttcgac gtggagtatc ggcgtatccg gcgttggacg agcccgtcta
     1081 ccttgcctct gccatcgatc tcgcgaaggt ttatgcgcgt cccaacaccg ccacagctcc
     1141 ggtcgggacg atccatcagg acagtatcgt accagcctat attcttaccg atgaattgtt
     1201 gggcaagcat ttcagcatcg tgggtacgac tgggtcaggt aaatcctgcg ttgtcgccac
     1261 gatcttgaat gccgtcatcg agcggaatcc gaatgcccat gtgatgttgc tcgatcccca
     1321 caacgaatat gcgagcgcat ttaaagaacg ggcggcatct cttagtccgg gtggggggtt
     1381 atatttacca tattggctat tcaacttcga agaattagtc gagattgtaa tcggggccga
     1441 tcacaacccc gagcaggtaa aaatacttgg cgaagcagtg ctgtctgcca agcaatctta
     1501 ttttgctaaa gtcggcctcg ataagttcgg gaccgtcgac acaccggccc catatcgcat
     1561 gtcagacgcg cttcgatttc tcgacacggc ggtgggcagc ctcaaccgcc ccgacagcgt
     1621 cgtcgcctat cagacggtca aaggacggat ccttgctctt cagaacgatg ctcgctacga
     1681 gtttgtattc ggcggacgct tgactttgcg cgatgagttg tcggatattc tttcacaact
     1741 cttccgaatc ccggtcaatg gtaagccgat tactattctc gacctctccg gcatcccatc
     1801 ggaagtattg aatgtagtgg tttccgtttt gtgccggctc gccttcgact ttgccttgtg
     1861 gagcgagacg ccggtcccga tcacgataat ttgcgaagag gcacaccgtt acgcacctcg
     1921 cgacaaggag cttggcttcg aggcggcaaa gcgggcgctg tttcgtatcg ccaaggaggg
     1981 ccgcaagtac ggcgtttcac tgtgtgtcgt cagtcagcgt ccgtcggatc tggccccggg
     2041 tctgctgtcg gagtgtaaca cgatgttcgc tttccgtatg accagccatg aggatcagga
     2101 catcgtgcgg agcgccgtcc ctggagcctc tcatggcctg atgaacttct tgccggcgtt
     2161 gcgcaacggc gaagcgattg ctgttggcga aggcgtggcg atgccgatgc ggatatgctt
     2221 tgcgccgctg cctgaggacc gccgccctaa gagcgccacc gcatctttta cgatcgcctg
     2281 gtcgaaagat agcgacggct cgcacatcga gcggaccgtc gagcggtggc gacgaggcat
     2341 gcgccacggc ccaggatagt ctgttcaacc cgctggaggg gagctagcgg ttgatctgcg
     2401 actgttggtc ttgcacgccc aggctttccg ccacatatcc tcggcgcatt ttcccgtcac
     2461 cccgcgatgg caggctatga cccaggaaac cagaagcttc caggcggaag tcagccgcct
     2521 actcgacatt gtcgcgcact cgctctacag ccaaaaggag atctttctac gcgagcttat
     2581 ttcgaatgcc tcggatgcgt gcgaccggtt gcggtacgcc gccttgaccg aaccggcatt
     2641 ggccgaaggc gataccagct accgcgtcgt attgagcccc gacaaatcgg ctcgaaccct
     2701 gaccgtggcc gataacggta tcggcatgaa ccgagcggag ctgatcgaaa atcttggcac
     2761 catcgcccgc tcaggcaccg ctgcgtttat tagccagctg tcgggggacg cgcgcaagga
     2821 tatgagcctg atcggccaat ttggggtcgg tttctactcg gcattcatgg tggccaaaaa
     2881 ggtcgaagtg aagagccgga aggccggcga gagcgaaggt tggcgctggg tctccgacgg
     2941 caagggtgag ttcacgatcg aacccttgcc cgaggcagcg cggggggcca cgatcgtcgt
     3001 gcacctgccc gagggcgagg acgagtatct tgagccggcg cgattgagca ggatcgtcag
     3061 cacttattct gaccatatcg gactgccgat cgtactcaaa gacggcggca aggaagagac
     3121 gatcaacacc gcctcggcct tgtggacccg tccgaaatcc gaaatcaccc aagagcagta
     3181 caacgagttc tatcatcacg tcggccacag cttcgatgtc ccgtggttga ccttgcatgc
     3241 caaggcggaa ggcgttctcg aatacacgag cctactcttt gtgccatcgg cgaaaccctt
     3301 cgacctgttc gacccggagc ggaagagccg ggttaagctc tatgtccggc gggtattcat
     3361 caccgacgag gggaccaatc tcttacccgc atatttgcgc ttcctgcgtg gcgtcgttga
     3421 cagcgaggat ctgccgctca acatcagtcg cgagatgctg caatcgaacc cgatggtggc
     3481 gcgtatccga cagcagttga ccaagcgaat attgacagaa ctgggcaaga aggcgaacga
     3541 gttgcctgag gaatacgtta agttctggga taatttcggc gcggtcctga aggagggcct
     3601 ttacgaggac cgtgagcatc gggaggaatt gctcacgctg accaggttcc gctcgaccgc
     3661 gcgtaacggg ctcgtttcgc tagacgagta tgtcacggcg atgagaccgg ggcaggaggc
     3721 gatctacacg atcaccggcg acagcctcga tgttatcaag cagagcccgc agctcgaagg
     3781 attccgcgcc cgcggcgtcg aggtgctgct gatgactgac ccaatcgacg agttctgggt
     3841 gccggcgatc ggctcattta aggacaagtc gttcaaatcc gcgacccgcg gcggtgtgga
     3901 cctcgacaaa atcacaccgc ctgaggatcg caaggacgag aaatccgagc cgccggccaa
     3961 gctcgcaagc ctgatcgcca tcttcaagct tgcgctcggc gacgcggtca aggacgttcg
     4021 tagttccgac cgtctgaccg acagcgccgt ctgtctcatc gccgacgaag gcgacatgga
     4081 catgcatttc gagcggctct tgaaacagca ccgccagctc gacacgatgt ccaaacgcat
     4141 cctcgagcta aacccgcacc ataagctgat cgagcgcctc gctgcgacgg tcggcgaggc
     4201 cggcgcctca gaccagcttt ccgaatttgc ctggcttctt ctggaccaag cccgaatcat
     4261 cgaaggcgaa cagctccctg acgctgcggc gttcgcgcgc cgtctgtcga ctgttctcga
     4321 gcgcgggttg tcggcagcgg cttgacgagg ggcaatcgac cccctgcggg tgcggcgtaa
     4381 atggagccgt aacgacgcga acgcgcagtc gggatcgaga gtccgccgcc gctcctcaac
     4441 ggcgcaccgc ctgacaggcg ctttgtcctg ctccatgcag gtggcgcctc gccgcggcgg
     4501 atcgtttggt cgcggccgac ggccgctggt cctttcgccc aaccgaccgc acgcgtaacg
     4561 ctcatatggc acggtcagag acattccgtg gcgatgaccg agagctagga gggacgatct
     4621 gggagcgatg cgttcatgct ccagctcgac ttcgcgatcc ctgggtcgtg tatgcttcag
     4681 gtgggatacc acgaatcgca gcgacacatc ggaaggctcg acgttaacat cacccgaggc
     4741 tgcatagcgg tgctcctcag gatgtgaccg tgattcagcg ctgcggcgga gcaagccgtc
     4801 gccacgcgta agtcggggcg acaggggcag accgaggcgg cgctaagcct gtcagcagca
     4861 cgatagagtt gacggatcgg gtccgaaagt cgcgcccagc ggcaagttac agcgaattaa
     4921 tgtgggtgaa agacgcctgt caccttactg gaccattttg tttgtctatc cactctgctc
     4981 gacgttcaac agcgagccgc cgcttaacag gaggaccaat tggcagaatc gataaacaac
     5041 tgtagccccc acccctggct aaacagaaag cgcgccgcag cgatcggttt cggtctcggc
     5101 gcgacattag cgctctcgcc cttcgtgtgg gccgaaaacc caccgctcgc gagcaacccc
     5161 tcatcggcgc cgcagacaat gacctctccg gtcgcgctga tccagcagca gagctttgcg
     5221 ccgctggtaa agaaggtatc gccggctgtg gtgaacatat ccgtctcgca aaaggccgat
     5281 gcggacgcga tggtggaaga gccggacatg tccccgtctc caggtttccc gaactcgccg
     5341 tttgacgagt ttctacgccg cttcttcgaa cagcagggcc cgggcggcca gcggcatctc
     5401 ttcccggaga ctccgggtgg acaggcccgg cgcatcgccc tcggctctgg tttcatcgtc
     5461 gatccgtccg gctatgttgt aaccaacagc cacgtggtcg gcgatgcgac caaagttgaa
     5521 gtaacgcttc aggatgatag caaatatacc gcaaaaatca tcgggcgcga tccgaagacc
     5581 gaccttgcgg tactcaagat caacgccgat aagccgttgc cttatgtaaa attcggcgac
     5641 agcagcgcgg cgcaggtcgg cgactgggtg atggcggtcg gcaacccgtt cggtctcggc
     5701 ggcacggtga caaacgggat tatttccgcc cgcggccgtg acattcactc cggtccgttc
     5761 gacgacttcc tgcagattga cgcgccgatc aatcgcggaa attccggcgg cccgactttc
     5821 aatctcgagg gtgaagtgat tggcatcaac acggcgatct actcaccgag tggcggtagt
     5881 gtcggcatcg gcttcgcagt gccttccaac gttgccaaga caatcatcgc tcaactcgaa
     5941 gagcacggca aagtaagccg cggttggctt ggggtgcaga tccaggaagt gacccccgca
     6001 atcgcggcga gcctcggctt gcacggagag aagggcgcgc tcgtcgccgt cgtgacctct
     6061 gacagtcctg gcgccaaagc cggcatcaag caaggcgacg tgatcctgtc attcaacggt
     6121 accgatgtga gccatttgcg tgacctgccg cggatggttg ccggaactgc acccgatacc
     6181 gtcgcgacta tgacggtatg gcgaaacggg gagaaggttg agttgcacac caaggtcggg
     6241 gaactcgcca atgagcgggt cgtctcggcc accaactcag gggaggacga agatcaacag
     6301 ccgccgaagg cggaggcgct aggcatgcat ttcgccccgt tgacggcgca gcttcgccgg
     6361 gagctccacg tggggaaaga cgcgcagggc gtcgtggtct cgcgggtcga cagcggcagt
     6421 gccgcggagg atgtcggcct gagcgagggc gatatccttg tcgcgatcga ccagcagccg
     6481 gtcaacaatc cgaaggaagc agcggagaaa ctgaaggaag tggcaaactc ctctaagaag
     6541 aacgccctcc tgttgttgaa ccgtcacggc gtcacgcaat atgtcggcgt aaatctcgcc
     6601 aataataacg gctagactta gcgcttatca accccctcgg gtctacccga gggggctgac
     6661 acgcgcttag cctgtgataa ttgttgggat gggaaggccc aagtagccct tcaataagga
     6721 aaattagatt cgaacaattt ttccaggatt cagaatattg gccgggtcga gcgccttttt
     6781 gatcgcgcgc atgacgctga gcgcttctcc gtgctcgctc accagaaaat ccatcttgcc
     6841 atatcccacc ccgtgctcac cggtgctggt tccttccatc gccagcgccc gcgccaccat
     6901 cctgtcgttg aggcggcccg cttccgcgat ctcgtccggg cgttggggat cgatcatgaa
     6961 cacgagatga aagtttccgt cgccgacgtg accgactaac gccgaaggga ttggtgagct
     7021 agcaaggtcc cgtttcgtct ccttgatgca ttcagcgagc ctcgagatcg gtacgcacac
     7081 atcagtggcc cagcctttcg agcccgggcg cagcgccaga gcggcgtagt aggcatcatg
     7141 cctcgcttgc cacagcttcg agcgctcctc ggatgtcgtc gcccagcgga agtcattgcc
     7201 gccgtattca tcggcgatag ccttaaccgt ctcggcctgc tcagctaccc cggccgtcga
     7261 gccgtggaac tcgaagaaca gagtcggagc caccttatgg tttagcttcg aatacttgtt
     7321 gatggcggtc atctgcgctt cgtcgagcag ctcgatgcga gcgaccggga cgccgttctg
     7381 gatcgtgaga attacggtgt tgaccgcgtc ttcgatggtc gggaaagcgc aaaccgcagc
     7441 tgaaatcgcc tccgggatgc cgtaaagtct aaccgtcacc tcggtgatga cgcctaaggt
     7501 accttccgaa ccgacgaaaa ggcgggtcaa atcgtatccg gctgccgatt tgcgggcgcg
     7561 gcgggcagtt cggatgacgc gcccgtctgc cagcaccacg gtcagcgtga ggacgttttc
     7621 gcgcatcgtt ccgtagcgca ccgcgttggt tcccgaggcg cgcgtcgcgg ccatgcctcc
     7681 aagcgacgca tccgccccgg gatcgatcgg aaagaacagc ccggtatcgc gcaaatgctc
     7741 gttgagctgc tttcttgtga cgccggcctc gacaaccgcg tcgaggtctt cggcgttgat
     7801 ccgtaagatc tgattcattt gcgacaagtc tatgcaaatg ccgcccgcca gagcggcgac
     7861 atgaccctcg agcgatgtcc ctgtcccaaa ggcgatgacc ggggtcttgt gattcgagca
     7921 aagcctgact atggccgcaa cttcctcggt gctgtgcgca aaggtgaccg cgtccggagc
     7981 atgcggcgtg tggtaggatt cgtccttgcc gtgctgggcg cagacagcgg ccgaggtcga
     8041 gagacggtcg ccgaggagtt ggcgcaattc cgccagcagg gaggcatcga cggcctcacg
     8101 atgaaaggcg agtgcgggca tggcgctgta gccaaaattt acaactaaac aggaagctca
     8161 agcattactc cctgcccggg tgcacggcaa ccatgatccg aaccaccgca tcttgcctgt
     8221 ccccgacacg atggtgtcgt gtcattgagc aaccatcatc caaacaccac ttttcccaaa
     8281 tagcggtggc gagttgggag gtcgtgatgg ctctggcggc ggtcacgctc gacgacaaat
     8341 acgttctcga ccagggccgg atttatctca ccgggacgca ggcgctggtg cgcctgccga
     8401 tgatgcagcg ccagcgcgac gtcgctgccg ggctgaatac cggttgcttc atatcaggtt
     8461 atcgcggctc gccgttgggc gggctggatc aagcgctgtg gagcgcgcgc cgctttctcg
     8521 agaaaagcca catccacttc caaccggcga taaacgagga gctcggcgcg acggcggtgt
     8581 ggggaagcca gcaggtcaat ctgtttcccg gatccaagta tgacggcgta tttgctctgt
     8641 ggtacgccaa ggggcccggc atcgatcgca gcggcgacgc gctcaagcac gggaacgccg
     8701 ccggatcagc tccttacggc ggagttctgt tgctggcggg cgacgatcac acctgcaaat
     8761 cctcgacttt gccgcaccag tccgaatatg ccttcatgga tgcgggcatt ccggt
//
LOCUS       DATLJT010000227         4485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_930331,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000227 DATLJT010000000
VERSION     DATLJT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930331"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VM910_05205"
     CDS             <1..203
                     /locus_tag="VM910_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HVI61977.1"
                     /translation="LEQLIQEAVVTVPRALIAETINVIAVLSGRGSQRRLAELACIEG
                     LRRSGDYDLVPAVDLAPAGVRT"
     gene            200..529
                     /locus_tag="VM910_05210"
     CDS             200..529
                     /locus_tag="VM910_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HVI61978.1"
                     /translation="MIDLPARINRAIAAIAAFVATTALAAPAFAAGSNMPWEQPLQQI
                     LQSIEGPVSKIIAVIIIIVTGLSLAFGDTAGGFRRLIQIVFGLSIAFAASSFFLSFFS
                     FGGGALV"
     gene            529..807
                     /locus_tag="VM910_05215"
     CDS             529..807
                     /locus_tag="VM910_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HVI61979.1"
                     /translation="MAEVASHELFPGFEAPLHRSLTEPILLAGAPRAFAILNGTLSAA
                     IGLGLRLWIAGLVLWGIGHVAAVWAAKRDPSFVEVVRRHVRYPQHLGA"
     gene            817..3285
                     /gene="trbE"
                     /locus_tag="VM910_05220"
     CDS             817..3285
                     /gene="trbE"
                     /locus_tag="VM910_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HVI61980.1"
                     /translation="MLSLAEYRNKPQSLADFLPWVALVAEGVVLNKDGSFQRTARIRG
                     PDLDSATPAELVGTTARLNNALRRLGSGWAVFIEASRNAAQSYPQSKFPDPVSALVDV
                     ERKAQFEEAGAHFESAYYLTFLYLPPAEEAARAEAWLYEGRRRDGGVDAHEVLKGFID
                     RTDRVLQLIEGFVPEAVWLSDEETLTYLHSCISTRRHRARVPEVPMYLDAILVDEALA
                     GGLEPRLGKAHLRTLTVLGFPSATYPGILDELNRLAFPYRWSTRAIALDKTDATKILT
                     RIRRQWFAKRKSVMAILKEVMTNEASALLDTDAHNKALDADAALQELGSDLIGQAYVT
                     TTVTVWDDDVRAADEKLRLVEKVIQGRDFTCIAESVNAVEAFLGSLPGHAYANVRQPP
                     VSTLNLAHMIPLSAVWAGPERNDHLDGPPLLFAKTEGATPFRLSLHVGDVGHTLVVGP
                     TGAGKSVLLALIALQFRRYPGSQIFAFDFGGSIRAAALAMGGDWHDLGGALSDDAVEP
                     VALQPLAAILDPAEQAWAAEWVAGILAREGVTVTPEAKEHLWSSLTSLASAPAIERTL
                     TGLSVLLQSGTLKRALQSYCVGGPWGRLLDGETEGLGEADVQVFETEGLIGTAAAPAV
                     LAYLFHRVERRLDGRPTLLIVDEGWLALDDPTFGAQLREWLKTLRKKNASVVFATQSL
                     ADIESSAIAPAIVESCPTRLFLPNERAIEPQITSIYRRFGLNDRQIEIIARATPKRDY
                     YCQSRRGNRLFELGLGDVALAFTAASSKSDQTAIARILAEHGHDGFAAGWLRHKGLDW
                     AANLLTPKRDQQQDLIQSDLEMTP"
     gene            3285..4022
                     /gene="trbJ"
                     /locus_tag="VM910_05225"
     CDS             3285..4022
                     /gene="trbJ"
                     /locus_tag="VM910_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HVI61981.1"
                     /translation="MRFLRRAVLVGLASGMVAFGAPSASAQMLVYDPSNYAQNVLQAA
                     RALQQINNQITSLQNQALMLLNQARNLASLPYSSLQAIDQSIARTEQLLSQAQRIAYD
                     INQIDRAFTQTYPQSYAGSTSSQQLFSDAQTRWQNALAGYQDALRVQAGVVQALDVTR
                     AQTDALVSSSQSATGMLQATQAGNQLVALQTRQLLDLTALIAAQSRAQSLAGARTTAS
                     DAQAQAQFNQFLSNGQSYQPQTVQMFH"
     gene            4034..4393
                     /gene="trbK-alt"
                     /locus_tag="VM910_05230"
     CDS             4034..4393
                     /gene="trbK-alt"
                     /locus_tag="VM910_05230"
                     /inference="COORDINATES: protein motif:HMM:NF039468.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative entry exclusion protein TrbK-alt"
                     /protein_id="HVI61982.1"
                     /translation="MSFKIVIRGVAYVVLAVALLATAIALNNRQYKPAGSLQAEPASA
                     EDDLDGELARCKALRTQAAEDARCKAVWRASRERSLNSKKLYQDRAPDALPPAPNANE
                     PAGGRLSSPSDRTVQPQ"
BASE COUNT          778 a         1549 c         1343 g          815 t
ORIGIN      
        1 ggctcgagca gctcattcag gaagccgtgg tcactgtgcc gcgcgccctg atcgccgaga
       61 ccatcaacgt cattgccgtg ctctcgggtc gcggttcgca acgccgcctg gccgaacttg
      121 cctgcatcga aggtttgaga cgtagcggcg attacgatct cgtgcctgcc gtcgatctcg
      181 cacccgctgg agtccgcaca tgatcgatct gcccgcccgc atcaatagag cgatagccgc
      241 aattgcggca ttcgtcgcaa cgactgctct ggccgcgcct gcgtttgccg cgggctccaa
      301 catgccgtgg gagcagccgc tccaacaaat cctgcaatcg atcgagggcc cggtctccaa
      361 gatcatcgcg gtgatcatca tcatcgtcac cggcctgtcg ctcgcctttg gcgacaccgc
      421 cggcggcttc cgccgcctca ttcagatcgt gttcggcctg tcgatcgcgt tcgcggcatc
      481 gtcgttcttt ctgtcgttct tttcattcgg cggcggagcg ctggtgtaat ggccgaagtc
      541 gcttcgcacg aactctttcc cggtttcgaa gcgccgctgc atcgctcgct gaccgagccg
      601 atcctgcttg ccggcgcgcc acgcgctttc gccatcctca acggcaccct ctccgcggcg
      661 atcggcctcg gcttgcgcct gtggatcgcc ggtctcgtgc tgtggggcat cgggcatgtg
      721 gccgccgtct gggccgccaa gcgcgatccg tccttcgtcg aagtggtgcg ccggcacgtg
      781 cgctacccgc agcatctcgg cgcgtgagct gacgctatgc tgagtctcgc cgaatatcgc
      841 aacaaaccgc aaagccttgc cgacttcctg ccctgggtgg cgcttgtggc cgaaggcgtg
      901 gtcctgaaca aggacggcag ctttcagcgc accgcgcgca tccgcgggcc cgacctcgac
      961 tcggcaacgc ccgctgagct cgtcggcacg acggcacggc tcaacaatgc attacggcgc
     1021 ctcggctccg gttgggccgt ctttatcgag gcaagccgca acgcggccca gtcctatcca
     1081 caaagcaagt ttcccgatcc agtctcggcg ctggtcgatg tcgagcgcaa agcccagttc
     1141 gaggaggcgg gcgctcactt cgaaagcgcc tattacctga cattcctcta cctcccgccg
     1201 gctgaagaag cagcccgggc cgaggcttgg ctctacgagg gccgcaggcg ggatgggggc
     1261 gtcgatgccc acgaagttct caaaggcttc atcgatcgga ccgatcgagt gctgcagctc
     1321 atcgaaggct tcgtgccgga agcggtttgg ctctcggacg aggagacgct gacctacctg
     1381 cattcctgta tctcgaccag gcgccatcgc gcccgcgtcc cggaagtgcc gatgtatctc
     1441 gacgccatcc tggtcgacga ggcactcgcc ggcgggctcg agccgcgact cggcaaggcg
     1501 cacctgcgca cgttgacagt gttgggattc ccgtctgcga cctatcccgg gatcctcgac
     1561 gagctcaatc ggctcgcgtt tccctaccgc tggtcgaccc gggcgatcgc gctcgacaag
     1621 accgacgcca ccaaaatcct cacccgcatc cggcgccagt ggttcgccaa acgcaaatcc
     1681 gtcatggcga tcctcaagga ggtgatgacc aacgaagcgt cggccctgct cgacaccgac
     1741 gcccacaaca aagcgctcga tgccgacgcg gcgctccaag agctcggttc tgacctgatc
     1801 ggccaggcct acgtgaccac gaccgtcacg gtctgggatg acgacgtccg ggccgccgac
     1861 gaaaagctgc gcctcgtcga gaaagtgatc caaggccgcg acttcacctg catcgccgag
     1921 agcgtcaacg ccgtcgaggc cttcctcgga agcctgccgg gccatgctta cgccaatgtc
     1981 cgccagccgc ccgtctcgac gctcaatctc gcccacatga ttccgctctc ggccgtgtgg
     2041 gccggacccg agcgcaacga ccatctcgac ggcccgccgc tgctgtttgc caagaccgaa
     2101 ggcgcgacgc cattccgtct ctcactccat gtcggcgatg tcggccacac cctcgtcgtc
     2161 ggaccgaccg gcgccggcaa gagcgtactg ttggcgctga tcgcgttgca gttccgacgc
     2221 tatcctgggt cccagatctt cgcgtttgat ttcggtggat caattcgtgc ggcggcgctc
     2281 gccatgggcg gcgactggca cgacctcggc ggcgcgctct ccgacgatgc ggtcgagccc
     2341 gtcgctttgc agccgctggc cgcgattctc gatccggcag agcaagcctg ggcggccgaa
     2401 tgggttgcgg gaatcctcgc ccgggaaggg gtgacggtca cgccggaggc caaagaacac
     2461 ctgtggtctt cgctgacatc gctcgccagc gcgccggcga ttgagcgcac gctgacgggc
     2521 ctctccgttc tgcttcagtc cggcacgctc aagcgcgcgc tgcagtctta ttgcgtcggc
     2581 gggccgtggg gccggttgct tgatggcgag accgagggcc tcggcgaggc tgatgttcaa
     2641 gtcttcgaaa ccgagggact gatcggcacc gccgcggctc ccgccgtgct cgcctatctg
     2701 ttccatcgcg tcgagagacg cctcgacggc cggccgacat tgctgatcgt cgatgagggc
     2761 tggctcgcgc tcgatgatcc gacgtttggt gcgcagttgc gcgagtggct caagacccta
     2821 cgcaagaaga acgccagcgt tgtgttcgca acccagtcgc tcgcggacat cgagagcagt
     2881 gcgatcgccc ccgccattgt cgagagctgc ccgacgcgtt tgttcctgcc gaacgagcgc
     2941 gccatcgagc cgcagatcac atcaatctat cggcgcttcg gcctcaacga tcgtcagatc
     3001 gagatcatcg ctcgcgccac ccccaagcgc gactattact gccagtcgcg ccgcggcaat
     3061 cgcttgttcg aactgggact cggcgacgtc gcgctcgcct tcaccgccgc ctcatccaag
     3121 tccgaccaga ccgcgatcgc ccgaatcctg gccgagcacg gccacgatgg ctttgctgcc
     3181 ggctggctgc gccacaaggg cctcgattgg gccgcgaatc tcctcactcc gaaacgtgac
     3241 caacagcagg acttgatcca atcagacttg gagatgacgc catgatgcgc tttctgcgtc
     3301 gcgcggtgtt ggtcggcctt gcatccggga tggtggcgtt cggagcaccg tcagccagcg
     3361 cgcagatgct cgtctatgac ccctcgaact acgcgcaaaa cgtcctccag gcggctcgcg
     3421 ccctgcagca gatcaacaat cagatcacca gcctgcagaa ccaagccttg atgctgctca
     3481 atcaggcgag gaacctggcg agcctgccgt attcctcgct ccaggccatt gaccagtcca
     3541 tcgcgcgcac cgagcagctg ctgagccaag cccagcgtat cgcctacgac atcaatcaga
     3601 tcgacagggc ttttacacaa acttatccgc agagctacgc cggctcgacc tcatcgcagc
     3661 agctgttcag cgatgcgcag acccgctggc agaacgcgct cgctggatac caggatgcgc
     3721 tgcgggtcca ggccggcgtg gtgcaggcgc tcgacgtcac gcgggcgcag accgacgcgc
     3781 tcgtttcatc gagccaatcg gccacgggaa tgctgcaggc cacccaggcc ggcaaccagc
     3841 tcgtggcttt gcagacccgc cagctcctcg acctgacagc gctcatcgcc gcgcagtccc
     3901 gcgcacagag tctcgccggc gcccgcacca cggccagcga cgcacaggcg caagcgcagt
     3961 tcaaccaatt cctaagcaac ggtcaaagct accagccgca gaccgtgcag atgttccatt
     4021 aaggagcgcg acgatgagct tcaagattgt catccgcggc gtcgcttatg tcgtgttggc
     4081 cgttgcgctc ctggcaaccg caatcgcgtt gaataatcgt caatataagc cggcaggctc
     4141 tttgcaggcc gagcctgcgt ctgcggaaga cgaccttgat ggcgagcttg cgcgctgcaa
     4201 ggcgctccgc acgcaagccg ccgaggacgc taggtgcaag gcggtctggc gggccagtcg
     4261 cgagcgttct ctcaactcca agaagctgta ccaggaccgc gcgcctgatg ctcttccacc
     4321 ggcacccaac gccaatgaac cagcgggcgg acgcctttcc tcgccgagcg acaggacagt
     4381 gcagccgcaa tgaacaccgg catcatcgac cgtttcaccg aggtcttcac gagttacatc
     4441 gactcgggct tcggccttct cggtggcgag gtcgcgtttc tggct
//
LOCUS       DATLJT010000228         9276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1849384,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000228 DATLJT010000000
VERSION     DATLJT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849384"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="VM910_05235"
     CDS             <1..1405
                     /locus_tag="VM910_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVI61983.1"
                     /translation="LGYGWNVLRVGDANDIDRIEHALDIFHKTKGRPTFIILDSHIGY
                     GSPHKQDTAAAHGEPLGEEEVRLTKRDYGWPEDAKFLVPDAVRAHFAAGTGARGAQAR
                     GRWTELFAAYRSKYPQLATEVDLMQRRELPAGWDRGLPVFPADPKGIAGRDASGKVLN
                     VFAQNIPWLLGGSADLASSTRAALTFEGAGDFEPGTPGGRNVHFGIREHAMAAAVNGL
                     SLSKLRAFGATFFIFSDYARPAIRLSALMELPTIFVFTHDAMGDGEDGPTHQPVEQLA
                     SFRAEPGLVVLRPADANEVVEAYRYVMQLRHEPAVLALSRQPLPTLDRSKYAPASGLA
                     RGAYVLADPPAGKPEVILIASGSEVSLCVNAHEELLAQGIRSRVVSMPSWDIFEHQTQ
                     AYQDSVLPPDVTARVAVEQASTFGWERYVGRSGRVIGMKTFGASAPLKELLRKYGFEP
                     GRVTEVVKELLGKGKKN"
     gene            1434..2111
                     /locus_tag="VM910_05240"
     CDS             1434..2111
                     /locus_tag="VM910_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI61984.1"
                     /translation="MTKTPDVVFLFDVDNTLIDNDRVQAHLSEHLEQTYGAATRDRYW
                     EIFEQLRSELGYADYLGALERYRMEDLHRPELLRMSNWLVDYPFAERLYPHALDAVKY
                     VQQWGTVVILSDGDAVFQPRKVERSGLWHLFDNRVLIYIHKEQELDDVGRLYPADHYV
                     LIDDKLRILSAVKKFWGDNVTTVFPKQGHYALDSKVLAEYPPADIELASIGDLINYDL
                     SSFLKKN"
     gene            2196..2696
                     /locus_tag="VM910_05245"
     CDS             2196..2696
                     /locus_tag="VM910_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVI61985.1"
                     /translation="MAFTVRSNSFKDGDYLANTHILSADFGFGCAGNNQSPHLAWSGA
                     PAGTKSFAITCFDPDAPTGSGFWHWLVVNIPPNVTELALDAGNPKAPQLPRGALQTRT
                     DFGTPGYGGPCPPAGDHPHRYLFTVFAVGTDSLQATADTSAAVIGFQLHFNTLAKAAI
                     MGLYKR"
     gene            complement(2720..3142)
                     /locus_tag="VM910_05250"
     CDS             complement(2720..3142)
                     /locus_tag="VM910_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI61986.1"
                     /translation="MALRQASRHVTQFYDQLLASSGLRTTQFSILIRLRLAGPMTINA
                     LAKALVMDRTTLGRNILPLEREGLIDVVSGRADRRSKILRLTKAGAARLRAARAGWTQ
                     AQAKFEAAFGGRRAAGLRALLQAVTATDLGAVPSGAGD"
     gene            complement(3576..4637)
                     /locus_tag="VM910_05255"
     CDS             complement(3576..4637)
                     /locus_tag="VM910_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVI61987.1"
                     /translation="MEQSSSLRLFGRVFLVAILVLVAVWILRPLLPALAWAGVLALAT
                     WPAREWLIRKGMTTSGAAISLTLLVGILIVGPLIVVAIEMAREAVVVVRTVHELKEIG
                     LGTPSWVLQVPFLGDYVASWWRDHLADPDAAKELLGRAESLDFIHWTRSLGSEAVNRL
                     VILAFTLLTLFFAYRDSPTIASQSRIIADRLFGPSGEHLGEEAIVAIRATVNGLVLVG
                     LAEGAVLGFAYAIAGVSHPVLFGFATALLATVPFGATTVFVIACFTLVIQSRITAAVL
                     LFVFASIVVFVADHFVRPALIGASTRLPFLWVLLGIFGGLETFGLIGLFVGPAIMAAV
                     IAIWREGAKPATHDAPVGA"
     gene            complement(4720..5115)
                     /locus_tag="VM910_05260"
     CDS             complement(4720..5115)
                     /locus_tag="VM910_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61988.1"
                     /translation="MPRIKEKALKAELAKHGFESIAVKRELPAGRVELEANKLHPVHV
                     EGGESIYAPIPVSLSVELDARGRVKSIDGGTPDPAAVADATRYVKTLRDSGQLAAAGE
                     QQPASGLTHQIERDAQGRQVLRRKRFSIS"
     gene            complement(5180..6694)
                     /locus_tag="VM910_05265"
     CDS             complement(5180..6694)
                     /locus_tag="VM910_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI61989.1"
                     /translation="MATKRSKRAAKTSAAKSKRRGKARAAAAEARPKGVINAWEQDPG
                     DGAQPSGGQVIQRPVPVLREQPFSIRIVNPASAPEAKPHSPGTADFRYWTAAEALRRG
                     ADFWGALLPGTSWEVGSILPVDLDFGIDLNAFYDRQGLKFFHGSAAGRTVFSGESPDV
                     VCHELGHAVLDSLKPQLFDAASMEVAAFHESFGDMSAILSALQLPSLREGVLAETGGV
                     LHRASRLSRLAEQLGWAIRQSVPSAVEADCLRNAVNTFFYRDPDTLPTTTPATSLSSE
                     PHSFSRVFTGAFFEGLAGMLAMKDTKNEAALLEVSQDIGAILVEGIRAASVVPTFFSQ
                     VAATMLGVAATRFSAQGYEVPLRSGFVRHGILAPSMAVAATHAPVRAAAVAASTASTT
                     LPKLQLSVAEYGLGVPSIVVYAAAEPKRLQAAGAALAVGPAPAPGQDQAAKSFFEDLL
                     RRGRLKVARTGKPAAEVVRSSAPLTHETHTHELRREGRQMVLRRVRIDCRGCHC"
     gene            6995..8098
                     /locus_tag="VM910_05270"
     CDS             6995..8098
                     /locus_tag="VM910_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI61990.1"
                     /translation="MAASMRSARLHEPGQPLRIDTVETPKPRPRDVLVRVKACGIIPN
                     MNAIFSGRLWNYLPPLPASVGLDAAGVIAKVGGEVTDVAVGERVYVNPWLSCGMCSYC
                     RAGEPMLCSAAAFQGYFGFFPHSIRQLLDYPYGGFSEYITASPQRLVHLPPQVTFEQA
                     ARFGYLGTSYAALRLAQVRGGSWVAINGITGTLGVGATLLALAMGATRILGLGRNRDV
                     LAQLKALAPDRIEVLALGDAPIADWMQRHTDQLGVDVLIDCSARSAAAANTAEALAAL
                     KRGAIAVNIGALTEPLAIQPIRFMTARLQFRGSNWFTTGEGQSMTEMAQVGMLDLTKL
                     VTHAYPLAKVNDALAEVQTRPGGFVNIVVHPDH"
     gene            8158..9144
                     /locus_tag="VM910_05275"
     CDS             8158..9144
                     /locus_tag="VM910_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI61991.1"
                     /translation="MIGIARLTRRGLAAALLVIGIDFAAMPVRAQLAPSQPIKIIVGV
                     PAGGLGDLAARILAQRLSENGHPTIVENRLGGNGIIATDAVAKSRADGYTLIMANHST
                     LAILPHITKIGYDPEKDLTPIALMLAAPNVAIVKAALPVRSMRELVAYGKMNRLTNAT
                     QGIGASGHLIGEQFKQLTGVDLVHVHYRGAAFALQDVIAGHVDMMFDVVALTREHALS
                     GQVRALAVLSPQRNPVLPDVPTSAEVGFAALQGGAWFGLMAPAGTARTVVDWLNGETR
                     KAFETLEVRERLMRQGLQLPVGSPEEFAALIAAESKRWGEVIRTGGIKLEGN"
BASE COUNT         1725 a         2934 c         2921 g         1696 t
ORIGIN      
        1 cctgggttac gggtggaacg ttctgcgcgt cggcgacgcc aatgacatcg accgcatcga
       61 gcacgccttg gacatctttc acaagaccaa gggccgcccg accttcatca ttctggacag
      121 ccacatcggc tacggctcgc cacataagca ggataccgcc gcggcgcacg gcgagccgct
      181 gggcgaggag gaggtgcgcc tcaccaaacg cgactacggc tggcctgagg acgccaagtt
      241 cctggtcccc gacgccgtgc gcgcccactt cgccgcgggg actggcgccc gcggcgcgca
      301 ggcccgcggc cgatggaccg aactgttcgc ggcctaccga tcgaaatatc cgcagctcgc
      361 gaccgaggtc gacttgatgc agcgacgtga gctgccggcc ggatgggatc gcgggcttcc
      421 cgtctttccc gccgacccca agggcatcgc gggtcgcgat gcgtcgggca aagtgctcaa
      481 cgtgtttgcg cagaatatcc cctggttgct gggcggatcg gcggatttgg cctcctccac
      541 cagggcggcg ctgacgttcg agggggccgg agacttcgag cctggaactc ccggcggcag
      601 gaacgtgcac ttcggcatcc gcgagcacgc catggccgca gccgtcaacg ggctctcgtt
      661 gtcgaaactg cgcgccttcg gtgccacctt cttcattttc agcgactatg cgcgcccagc
      721 cattcggctc tctgcgctga tggaactgcc caccatcttt gtgttcaccc acgacgccat
      781 gggtgatggt gaggacggcc cgacgcacca accggtggag cagctcgcgt ccttccgcgc
      841 cgaacctggc ttggtggtgc tgcggccggc ggacgccaac gaggtcgtgg aggcctatcg
      901 ctatgtcatg cagctgcggc acgagcctgc ggtgctggcg ttgtcgcgtc aaccgctgcc
      961 gacattggac cgcagcaagt acgcgcccgc atccgggctc gcgcgcggcg cctatgtgct
     1021 cgccgatccg ccggcgggaa agcccgaagt gatcctcatc gcgtccggca gcgaggtcag
     1081 tctctgtgtc aacgcccacg aggagctgct cgcgcagggt atccgctcgc gcgtggtctc
     1141 gatgccgtcc tgggatattt tcgaacatca aacgcaagcg taccaggaca gcgtgctacc
     1201 gcccgacgtc accgcgcgcg tcgcggtgga gcaggcttcg accttcggtt gggaacgcta
     1261 cgtcggcaga tccggccgcg tcatcggcat gaagacgttt ggcgcctcgg ccccgctcaa
     1321 ggagctgctg cgaaaatacg ggttcgagcc cgggcgcgtc accgaggtcg tcaaagagct
     1381 gctcggtaag ggcaagaaga attgaagggc gtacaaaagc aggctgggat gcgatgacca
     1441 agacgcccga tgtcgtcttt ctgttcgacg tcgacaacac gctcatcgac aacgatcgcg
     1501 tgcaggccca tctcagcgag cacctcgagc agacctacgg cgccgcgacc cgcgaccggt
     1561 actgggaaat ttttgagcaa ctgaggagcg aactcggata cgccgattat ctcggcgcgt
     1621 tggagcgtta ccggatggag gacttgcacc gccccgagct acttcggatg tcgaactggc
     1681 tggtcgatta tccgttcgcc gagcgccttt atcctcatgc gctggatgcg gtgaagtacg
     1741 tacagcaatg ggggaccgtc gtcatcctct ccgacggcga cgccgttttc cagccgcgca
     1801 aggtggaacg atccggtctg tggcacttat tcgacaatcg tgtcctcatt tacatccaca
     1861 aggagcagga gctcgacgat gtggggcgtc tctatcccgc cgaccactat gtcctgatcg
     1921 atgacaaatt gcgcatccta tccgcggtga aaaaattctg gggcgacaac gtcacgaccg
     1981 tgtttccgaa acagggtcat tatgcgctcg actccaaggt actggccgag tatccaccgg
     2041 cggatatcga gctcgccagc atcggcgatc taatcaatta cgatctgtct tcattcctca
     2101 agaagaactg aaaggcagac gtcgcgtact gcaaagccgg cggagcaaga tcgctgccac
     2161 ctcagttttg ttggaagaca cgatggagac ggcgtatggc gttcacggtc aggagcaaca
     2221 gtttcaagga cggcgattat ctcgccaaca cccatattct ctcagccgac ttcggcttcg
     2281 gctgcgcggg caacaaccaa tcgccgcatc tggcgtggtc gggagcgccc gccggcacga
     2341 agagcttcgc cattacctgt ttcgatcccg atgcacccac gggcagcggg ttctggcact
     2401 ggctggtggt gaacatcccg ccgaatgtaa ccgagcttgc gcttgatgcc ggcaatccga
     2461 aggccccgca gctgccacgg ggagccctgc agacccgcac cgactttggc acgcctggtt
     2521 acggcggccc ctgtccgccc gcgggcgacc atccgcaccg ttatctgttc acggtgtttg
     2581 cggtcgggac cgatagcctg caggcgacgg cggatacctc agcagccgtc atcggctttc
     2641 aactccattt caatacgctg gccaaggcgg cgatcatggg cctctataag cgatgatcag
     2701 acgtcgaggc gcgatcggct cagtctccgg cgccggaggg cacggcgccg agatcggtcg
     2761 cggtgacggc ctggagcaga gcacgtagcc ctgcggcgcg ccggccgccg aatgccgcct
     2821 caaattttgc ctgcgcttgg gtccagcccg cgcgggccgc gcgcagccgc gccgcgccgg
     2881 ctttggtcaa ccgcaggatc ttgctccttc ggtcggcgcg gcccgacacg acgtcgatca
     2941 atccttcgcg ctcgagcggc aggatattgc ggccaagcgt ggttcgatcc atgaccaggg
     3001 ctttggccag ggcattgatc gtcatcggtc ccgcgagcct cagccttatc aggatcgaaa
     3061 actgggtggt gcgcagcccg ctcgacgcca gcaattgatc gtagaattgc gtgacgtgcc
     3121 ggctcgcttg gcgcagcgcc aagcaattgc agacctcggg ccgcggacgg gcgtcggagc
     3181 gcctcgctaa atcgtggcct gattttgcgc gcatatgcgc ttatatgggc gcgtctgtct
     3241 tggtgtcaat tgggccgaca cgctgcccgc tgcgcgaagg cgccgcgcaa ctggccgcgc
     3301 aggtctcgag cttagcctgt tccaaccagg caataaaaat gcgcggcaac atcgccatgc
     3361 ccccgttgca tgcgtcgccc tgcatcgtct ctgctcggca ggtttcggtg ctcgggatac
     3421 gcctcgtggc tggcaccatt gcgatttcgc ggctgcaccc taacgcatgg tgcttcggcc
     3481 tgcgttgttg gtttttgctg tcattgttcc aatcttgcgc aaatggcccg cgatgtgctt
     3541 gcggcgggca gggaccgctg cgggtccgag cgccctcacg cgccaaccgg cgcatcgtgc
     3601 gtggccggct tggcgccctc ccgccagatt gcgatgacgg cagccatgat cgctggtccg
     3661 acaaacaatc cgatcaggcc aaacgtctcc aagccgccaa agatgccgag gagaacccac
     3721 aggaaaggca ggcgagtcga tgcgccgatc agcgcagggc ggacgaaatg atcggcaacg
     3781 aagaccacga ttgatgcgaa cacaaaaagt aagaccgccg cggtgatgcg ggactgtatg
     3841 acgagggtga agcaggcgat cacaaacacc gtcgtcgctc cgaatggaac tgttgccaga
     3901 agcgcggtcg cgaagccgaa cagaacgggg tggctcacgc ccgctatcgc ataggcgaat
     3961 ccgagcaccg ctccctccgc caatccaacc agaacaagac cgttgaccgt tgcgcgaatt
     4021 gcaacgatgg cttcctcgcc gagatgctct ccggatggac cgaagagacg atcggcgatg
     4081 attcggcttt ggctcgcgat tgttggacta tcccgatatg cgaagaaaag cgtcagcaac
     4141 gtgaatgcca agatcactaa gcgattgact gcttcgctgc cgaggcttcg cgtccagtga
     4201 ataaagtcca gggattcggc tcgtcccaaa agctccttgg ccgcatccgg atccgccagg
     4261 tgatcccgcc accacgatgc gacatagtcg cccaaaaaag gaacttgcag cacccaactc
     4321 ggcgtgccca gtccaatttc cttaagctcg tgcaccgttc ggacaacaac gacggcttct
     4381 ctcgccatct cgatggctac aacgatgagc ggaccgacga tcagaatgcc gacgagaagc
     4441 gtgagtgaaa tcgcggcgcc cgatgtggtc atacctttgc ggatcagcca ctcgcgagcg
     4501 ggccaggtcg cgagggcgag cacgccggcc catgcaagtg ccggcaagag cggccgcaaa
     4561 atccaaaccg cgaccagcac caatatcgcg acaagaaaga cgcggccgaa caaccgcaga
     4621 gacgatgact gctccatgaa aggacccccc acgtcgatcc cttacatctc gccaagaaag
     4681 ctcttgggag taaaagctcg cctctgtccg gccatggccc tagctgatcg agaagcgttt
     4741 gcggcgcagg acttgccggc cttgcgcgtc acgctcgatc tggtgagtta gcccggaagc
     4801 cggctgttgt tcaccagcgg cggccagctg cccgctgtcg cgcagcgtct tgacgtagcg
     4861 cgtggcatcc gcgaccgccg ccggatcggg cgtaccgccg tcgatcgact tgacccggcc
     4921 gcgcgcgtca agctcgacgg atagcgacac cggaatgggc gcgtagatcg attcgccgcc
     4981 ctccacatgc acggggtgga gcttgttggc ttcgagctcg acgcgtccag ccggtaattc
     5041 gcgcttgacc gcgatcgact cgaagccgtg tttggcaagt tccgccttca gcgccttttc
     5101 cttgatgcgc ggcattggcc cgccattttt cggtccccga attcccctcg cgcatcatat
     5161 aggccccggg agtgcgttct caacagtggc aaccccggca gtcgatgcga acccggcgca
     5221 gcaccatctg ccgtccttcg cgccgcaatt cgtgggtatg ggtttcgtgg gtgagcggcg
     5281 cggaagagcg tacgacttcg gccgctggct tgccggttct cgcgaccttg agccgccccc
     5341 gtcgcagcag atcttcgaag aaggacttgg cggcctgatc ctggcccggc gcgggcgccg
     5401 gcccgacggc aagcgcggcg ccggcagcct gcaggcgctt cggctcggcg gcggcataga
     5461 ccacgatgga aggaacaccg aggccatact cggccacgga aagttgcagc ttcggcagcg
     5521 tcgtcgatgc cgtgctcgcg gcgacggccg cggcgcgcac tggcgcatgc gtggcggcca
     5581 ccgccatcga tggcgccaag atgccgtggc ggacgaaccc gcttcgcagc ggtacttcat
     5641 agccttgagc cgagaatcgg gtggcggcga caccgagcat ggtcgcagca acctggctga
     5701 aaaaggtagg aacgacggac gcggcgcgga tgccttcgac cagaatcgca ccgatatctt
     5761 ggctgacttc cagcagcgcg gcctcgttct tcgtatcctt catggccagc atgccggcca
     5821 atccttcgaa gaaggcgccg gtgaagacgc gcgagaagga gtgcggctcc gagctgagcg
     5881 acgtcgccgg cgtcgtggtc ggaagcgtgt cgggatcccg gtagaagaag gtgttcaccg
     5941 cgttgcgcaa gcagtcggcc tcgaccgcgg aaggaaccga ttgccggatg gcccagccga
     6001 gctgttccgc caaccgtgat agccgggagg cgcggtgaag aacaccgccg gtttccgcca
     6061 acactccctc gcgtaggctc ggcagctgaa gcgccgatag aatcgcgctc atgtcgccga
     6121 acgattcgtg gaaggcggca acctccatac tcgcggcgtc gaacaattgc ggcttgaggg
     6181 aatcgagaac ggcatgcccg agctcgtggc acacgacgtc cgggctctca ccggaaaaga
     6241 ccgttctccc cgcggccgaa ccatggaaga acttcaagcc ctgacggtcg taaaaggcgt
     6301 tgaggtcgat cccgaaatcg agatccacgg gcagtatgga gcctacttcc caggaggttc
     6361 caggcagcaa tgctccccag aaatcggcac cgcggcgcaa ggcttcggcg gcggtccagt
     6421 agcgaaaatc tgcagttccg ggcgaatgag gtttagcctc cggagcggag gccgggttga
     6481 caattcggat cgagaagggc tgctccctca gaacggggac cggcctttgg atgacttggc
     6541 ctccgctcgg ctgggcaccg tcgccgggat cctgctccca agcgttgatc acgcccttcg
     6601 gcctggcttc cgcggccgcc gcgcgcgcct tgcctctgcg cttgcttttc gcggccgacg
     6661 tcttcgccgc gcgtttcgat cgcttcgttg ccatctcatc tctcccattt ctgagtcgcc
     6721 gacatcaaat ccatgagcaa ggccgcactg gccctgatgg gcggcctttc catcatgatg
     6781 cattcagcag cgatacgacg tgagataaat cacagtagct cagctgaact ctgagagtcg
     6841 acgcctgcta atagccgttg gcaatggcga cgatgccgaa ggcgttttgg gaaaagtcgc
     6901 cccatggtcg acggaatatc ccaggtcttc gaatttcgct gtccttttcg cggtcagccg
     6961 atctaatttc tggcgggtga caggaggaaa atcgatggcc gcttccatgc gctctgcgcg
     7021 cctccatgaa cctgggcagc cgttgcggat cgatacggtg gaaactccga agccgcgtcc
     7081 gcgcgatgtg ctggttcggg tgaaggcatg cggcatcatt cccaatatga acgcgatttt
     7141 cagcggccgg ttgtggaatt acctgccgcc acttcccgcg agcgttgggc tcgatgctgc
     7201 cggcgtcatc gcgaaggtcg ggggtgaggt caccgacgtg gccgtgggtg agcgtgttta
     7261 cgtcaatccg tggctctcct gcggcatgtg ttcgtattgc cgcgcgggcg agccgatgct
     7321 ctgcagcgcc gctgccttcc agggctactt cggctttttt cctcattcca tccgccagct
     7381 cttggattat ccgtatggcg gtttttcgga gtacatcact gcgtcgccgc agcggctggt
     7441 gcacttgccg ccgcaggtga cattcgagca agccgcgcgc ttcggctatc tcggcacctc
     7501 gtacgctgcg ctgcgcctcg cccaggtgcg cggcggcagt tgggtcgcga tcaacggaat
     7561 taccggcacg ctgggagtcg gcgcgacatt actcgccctt gcaatgggag cgacgcgcat
     7621 tcttggcctt ggccgcaatc gtgacgtgct tgcacaattg aaggcgctgg cgcctgatcg
     7681 tatcgaggtc ctcgcgctcg gcgatgctcc gatcgcggat tggatgcaac ggcacacgga
     7741 ccagctcggc gtggacgtgc tcatcgattg ttccgcgcgc agtgccgccg cagcgaacac
     7801 cgcggaggcc cttgccgcac tgaagcgcgg cgcgatcgca gtcaatatcg gcgctttgac
     7861 cgagccgctc gcaatccaac cgatacgttt tatgaccgct cgcctgcaat tccgcggctc
     7921 gaactggttc acgacggggg aggggcagtc gatgaccgag atggcgcagg ttggtatgct
     7981 tgatctgacc aagctggtca cgcacgccta tcctcttgcg aaggtcaatg acgcgctggc
     8041 cgaggtgcag acgcgcccgg gcgggttcgt caatatcgtc gtccatcccg accactgata
     8101 gggcttactc gaacgaagga ttgatcaaac cgcccatcag tacacggagc aaacaggatg
     8161 attgggattg ctcggcttac gcgacgcggc cttgctgccg cgctgctcgt gatcggcatc
     8221 gatttcgcag ccatgcctgt gcgcgcacag cttgcgccgt cgcaaccaat caaaatcatc
     8281 gtcggcgtgc cggcgggtgg cctcggcgat cttgccgccc gcatcctcgc gcaaaggctc
     8341 agcgaaaacg ggcacccgac aattgttgaa aaccgccttg gcggcaacgg catcatcgcc
     8401 accgatgccg tcgcaaaatc gcgggcggac ggctacaccc tgatcatggc caatcattcg
     8461 acgcttgcga tcctgccgca catcaccaag atcggctacg accccgagaa agacttgacg
     8521 ccgattgcgc tgatgttggc ggcacccaat gtggccatcg tcaaggcggc gttgccggtg
     8581 agatcgatgc gggaactcgt cgcctacggc aagatgaatc gcctgacaaa tgccacgcaa
     8641 ggtatcggtg cgagcggcca tctcatcggt gagcagttca agcagctgac cggggtcgat
     8701 ctcgttcacg tccattatcg cggcgcggcg ttcgcattgc aggacgtcat cgcgggacac
     8761 gtcgatatga tgttcgatgt ggtggcgctc acgcgagaac acgcgctgtc gggacaagtg
     8821 cgggcgctgg cggtgttgtc gccgcagcgg aacccggtgc tgcccgatgt cccgacctcg
     8881 gcggaagtcg ggttcgcagc gctccaaggc ggcgcctggt tcgggctcat ggcgcccgcc
     8941 ggtacggcgc gcacagtcgt cgattggctc aacggcgaaa cccgcaaggc gttcgaaacg
     9001 ctggaggtgc gcgaacgcct gatgaggcag ggcctgcagc tgccggtcgg aagcccggaa
     9061 gaattcgccg cgctgatcgc cgccgagagc aagcgctggg gggaggtgat ccgcacaggc
     9121 ggcatcaagc tcgagggcaa ttgagcggca ggcgtgcaat tgcgatagcg cttgtagggc
     9181 accgcggcgc tcgcgggatc ggatgatgcg accattacgg gcgggcaacc agcagggact
     9241 ccacgttcgc gcctcccgcc atttgcgggt gcgatg
//
LOCUS       DATLJT010000229         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3228115,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000229 DATLJT010000000
VERSION     DATLJT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228115"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            593..2095
                     /gene="istA"
                     /locus_tag="VM910_05280"
     CDS             593..2095
                     /gene="istA"
                     /locus_tag="VM910_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI61992.1"
                     /translation="MRLYMTYRHSKNATVAAAKSGFSRATGYRIEADPRLPSQKKAPR
                     GRRRPDPLAEVWDGEIVPILKSAPGIRAIAVLDEIRRRHPQISAGIRRTLERRMRSWR
                     ALAGPEQDVIFRQEYEPGRLGLSDFTDMSVLGITVAGVVLGHRLYHFRLPFSGFEHAH
                     VVLGGESFVALAEGLQNALWALGGVPREHRSDSLSAAFRNLDRDAQEDLTLRYQALMR
                     HYDMMPTRNNLGVAHENGSIESSHGHLKKALQDALLLRGSRDFDHLDGYRRFVDEIVG
                     RRNANNRKRIELERPMLTALPKRRTADYEEKIVTVTSSGGFILRRVFYTAPSQLIGHR
                     LRVHLYDDRLECFLGATPMMTLRRGRPVSDSKGGHVVDYHHVIHALRKKPMALNNLVY
                     RDELFPRPAYARAFEALHQEVGDKRACKLTVELLSLAHDRGCEAELSELIDAELDAGR
                     LPDLAFLRERFAPNLASVPEIKVALVALSSYDELAAVCIVSPTSNREVVA"
     gene            2092..2916
                     /gene="istB"
                     /locus_tag="VM910_05285"
     CDS             2092..2916
                     /gene="istB"
                     /locus_tag="VM910_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI61993.1"
                     /translation="MTTTNPVVDAARVELLLHELRLPSIRLMWAKLAEQSDKEGWPAA
                     RFLAVLAEHEMADRGRRRLERHLVEARLPAGKTLATFDFEAVPMVSKAQVMALASGDA
                     WLKKGANILLFGPPGGGKSHLSAALGLALVENGWRVLFARTTDLVQQLQVARRELALE
                     SAIAKLDRYDLLILDDIAYVTKDQAETSVLFELIAARYERRSMLITANQPFGEWGKIF
                     PDQAMTLAAIDRLVHHSTILEMNVESYRRREALDRKRGRGRPPTHATINKTENAEN"
BASE COUNT          615 a          984 c          947 g          589 t
ORIGIN      
        1 caagccgtcc cgctcgtgcc ggatctggcg cgtgcgatag aaattgtcca gtgagacgcc
       61 tattgccatc gcaacctcgg tcgaggaata gggcggcacc atcgtctggg gactattcgg
      121 aatgcgcaaa tgcataagtt cgcaacaccg cgtcacttct tcctccttcg acgaaatcga
      181 tggaacatgg gagcagacaa gaacgaagat gaaaagcccg cgcatcagac gtggggaatt
      241 cggctagttt agagtgcaaa taggatgcaa gcggcgctgt cacgtcgcgc attcgcgcct
      301 attctgcgtc gaggtcagct gtagaatcca acctgcactc agaccgttat gagcggcagt
      361 gccgcgcgac aatcaaggtg agacagcagc gtcaatcagg atgagaacgc gccgaggctg
      421 gacggacgga gcgagggcgt agcccgagcg ctgttcgtcc agcctcggcg acgcactttt
      481 ccccaagggg gtcagtgcgc tctggcggtc gtgagcgacc gggtcaagtg tctgattcgt
      541 cttgaaattg aggaatcagc gattgcccgg ccttcacatc actgaccacc agatgaggct
      601 gtatatgact taccgacata gcaaaaatgc caccgttgct gccgccaagt ccggcttcag
      661 ccgggccacc ggctaccgca tcgaggctga tccgcggctg ccttcgcaga agaaagcgcc
      721 gcgaggccga cggcgtccgg acccattggc cgaggtttgg gacggcgaga tcgtgccgat
      781 cctgaaatcg gctcccggta ttcgggcgat cgcggtgctt gacgagatcc ggcgccgcca
      841 tccgcagatc agcgccggca ttcggcgcac gctggaacgg cgaatgcgca gctggcgagc
      901 cctggccggc cccgagcagg atgtgatctt tcgtcaggag tacgaacccg gccggcttgg
      961 cctgtccgat ttcaccgaca tgagcgtact cggcatcacc gtcgccggtg tcgtgctggg
     1021 acatcggcta taccacttcc ggctcccctt ctcgggcttc gagcatgccc atgtcgtgct
     1081 cggcggtgag agctttgtcg cgctggccga gggtctgcag aatgcgctgt gggcgctcgg
     1141 gggcgtgccg cgcgagcatc gcagcgatag tctctcggcg gcattccgca atctggaccg
     1201 cgacgctcag gaagatctga cgctgcgcta tcaggcgctg atgcgacatt acgacatgat
     1261 gccgacccgc aacaacctgg gtgtcgcgca cgagaatggc tcgattgaaa gttcgcatgg
     1321 gcatctcaag aaggcgctgc aggacgcact gctgctgcgc ggcagccgcg acttcgatca
     1381 cctggacgga tatcgccgct tcgtcgacga gatcgttggc cggcgcaacg ccaacaatcg
     1441 caagcgcatc gagttggagc ggccaatgct cactgcgctg cccaagcgcc ggacggccga
     1501 ttacgaggag aagattgtca ccgtcacctc cagcggcggc ttcatcctgc gtcgcgtctt
     1561 ctacaccgcg ccgtcgcagc tgatcggcca tcgcctgcgc gtgcatctct acgatgatcg
     1621 gctcgagtgc ttcctcggcg caacgccgat gatgacgctg cggcggggcc ggccggtatc
     1681 ggacagcaag ggcggtcacg tcgtcgatta tcaccatgtg atccatgcgc tgcgcaagaa
     1741 gccgatggcg ctgaacaatc tggtgtaccg tgatgagttg ttcccgcgtc cggcctatgc
     1801 gcgggccttc gaggcattgc accaggaagt gggcgacaaa cgcgcctgca agttgaccgt
     1861 cgagttgctg tcgctcgcac atgatcgcgg ctgcgaggct gagctttcgg aactgatcga
     1921 cgccgaattg gatgcgggtc ggttaccgga tctggcattc ctgcgtgagc ggttcgctcc
     1981 aaacctggca tccgtacctg agatcaaggt agcgcttgtc gcactcagca gctacgacga
     2041 actcgcggct gtctgcatcg tcagcccgac ctccaatcgg gaggtcgtgg catgacgacg
     2101 accaaccctg ttgttgatgc cgcccgcgtc gaactcctat tgcatgagtt gcgcctgccg
     2161 agcatcaggc tgatgtgggc caagctcgcc gagcaatccg ataaggaagg ttggccggcg
     2221 gcccgcttcc tcgcggtgct cgccgagcac gagatggctg atcgtggccg ccgccgcctc
     2281 gagcggcatc tcgtcgaggc gcggctgccc gccggcaaga cgctcgccac cttcgacttc
     2341 gaggccgtgc caatggtctc gaaagcgcag gtgatggcct tggcgtccgg cgacgcctgg
     2401 ttgaagaaag gcgccaatat cctgctcttc ggtccacctg gcggcggcaa gagccatttg
     2461 tcggcagcgc tcgggctcgc tttggtcgag aatggatggc gcgtgctgtt tgcccgtacc
     2521 accgatctcg tgcaacagct ccaggtcgct cgtcgcgagc tggcactcga aagcgcgatc
     2581 gccaagctcg accgttacga tttgctcatc ctcgacgaca tcgcctacgt caccaaggat
     2641 caagccgaaa ccagcgtact gttcgagttg atcgccgcac gttacgagcg gagatccatg
     2701 ctaataaccg ccaatcagcc atttggcgaa tggggcaaga tctttcccga ccaggctatg
     2761 acgctcgccg ccatcgatcg cctagtccat cactccacga tcctggagat gaatgtcgag
     2821 agctatcgcc gccgcgaggc gctcgatcga aagcgaggtc gcggcaggcc gccaacccac
     2881 gcgacaatca ataaaacaga gaacgccgaa aactgattga cgctccgcgt caatcaaaga
     2941 cttgccaaga cgtcggtcag cgtcaatgat ccctccgctc ggccgccctc gtctcatcca
     3001 gattgacgcg ccgttctcat ccagattgtc gcgccatagc aaccgatatg tgcagcctaa
     3061 agccaccgcg ccacatctcg actccaccac ccgcagtatt gctctaaccg atcacaagat
     3121 ttccgctcgg ccctc
//
LOCUS       DATLJT010000230         1568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1619719,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000230 DATLJT010000000
VERSION     DATLJT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619719"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VM910_05290"
     CDS             complement(<1..453)
                     /locus_tag="VM910_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI61994.1"
                     /translation="MKRREFITLVGCAAVWPLAARAQQRERPRRIGVLMGLTESDPEA
                     QSRIAAFRKTLQDLGWTEGRNVRTDYYWAAGDIDRTHALAKELVSSTPDVIVVNTPPG
                     LSALRVETHTIPIIFVQVLDASESTVVMNPARPESNVTGFTNFYEYGVS"
     gene            complement(450..1418)
                     /locus_tag="VM910_05295"
     CDS             complement(450..1418)
                     /locus_tag="VM910_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI61995.1"
                     /translation="MRPLIVILVCLVAVFGESPTTYAQERSHVPRVGYLALGRLATPT
                     VFAQKMREQGFVDGQTAAVEYRFADGRHERLLPLALELAEGQVDVIFAFGDEAIAAAQ
                     IATKTIPIVMFACDAVTTGFVASLARPGGNTTGVTCITTELSGKRVAVLDEMLPRLKR
                     FAVLYNPENKSKPMDFAQTREAANSLGISVDGFEAREPEDVDRIFAGFAASKPDGVSV
                     LDEAFTMLNARRIVELARRHGLPSMHSFREPVAAGGLLSYGPSQSEMTAIATTFIVKI
                     LRGAKPADLPVQQPTKFELVINLKAAKTLGIEIPPSLIARADEVIE"
     gene            1445..>1568
                     /locus_tag="VM910_05300"
     CDS             1445..>1568
                     /locus_tag="VM910_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVI61996.1"
                     /translation="MAAAEAESVKNNWGMAIAILDSTGHIVMLHKLDNTQYGSIA"
BASE COUNT          312 a          480 c          472 g          304 t
ORIGIN      
        1 gctcacgcca tattcataaa aattcgtgaa cccggttaca ttggattccg gacgagccgg
       61 gttcatcacg acggtgctct cggaagcgtc caggacctgg acaaagatga tcggtattgt
      121 gtgggtctcc actcgcagtg cagacaatcc gggcggggtg ttgacgacga tcacatccgg
      181 tgtcgagctc accaattctt ttgccagcgc atgcgtgcgg tcaatgtcgc ccgcagccca
      241 gtaatagtcc gtccggacgt tgcgtccttc cgtccaaccc aggtcttgca gcgtcttgcg
      301 gaacgccgca atgcgagatt gtgcctccgg atcgctctcg gtaagcccca tcagcacgcc
      361 gatgcgcctc ggcctctcgc gctgctgcgc gcgcgccgcg agcggccaca cggccgcgca
      421 gccaaccaga gtgatgaact cgcgcctttt cattcgatca cctcgtcggc gcgggcgatg
      481 agcgacggcg gaatttcgat cccgagcgtc ttggcggcct tgagattgat caccagttcg
      541 aattttgtcg gctgttggac cggcaaatcc gcgggcttcg cgcccctgag aattttgaca
      601 ataaacgttg tggcaatggc agtcatttcc gactgactcg gaccgtacga caagagacca
      661 ccggctgcga cgggttcgcg aaaggaatgc atgctcggca ggccgtgcct gcgtgcaagc
      721 tcgacgatgc gcctggcgtt gagcatcgtg aaagcttcgt cgaggacgga gacgccgtcg
      781 ggcttgctcg ccgcgaaacc cgcgaaaatt cgatcgacat cttccggttc acgtgcctcg
      841 aagccatcaa cgctgatgcc gagcgaattt gccgcctcgc gcgtctgtgc gaagtccatc
      901 ggcttgctct tgttctccgg attgtagagc acggcaaagc gcttcagccg tgggagcatt
      961 tcgtctaaga cggcaacgcg cttgccggaa agttcagtgg taatgcaagt gacgccggtg
     1021 gtgttgccgc cgggccgggc aagactcgcc acgaaaccgg tcgttacggc gtcgcaggcg
     1081 aacatcacga tggggattgt tttcgtcgcg atctgcgcag ccgcgatcgc ctcgtcgcca
     1141 aaagcgaaga tgacgtcaac ctggccttcc gccaactcta gcgcaagcgg caacagccgc
     1201 tcgtgccggc cgtctgcgaa gcgatactcg actgcggcag tttgtccgtc gacgaagcct
     1261 tgctcgcgca ttttctgcgc gaagacagtc ggcgtcgcga ggcggcccag cgccagatag
     1321 ccaacacgcg gcacgtgcga tctttcttgc gcgtatgtgg ttggcgactc gccaaacaca
     1381 gcgacaaggc aaaccaggat gacgatcagc ggtctcattc gatcaccagg cagccaaaaa
     1441 agtgatggcg gccgcggagg cggagtcggt gaagaacaac tggggcatgg ccatcgccat
     1501 tctcgacagc accggccata tcgtgatgct ccacaagctc gacaacaccc agtacggctc
     1561 gatcgcgg
//
LOCUS       DATLJT010000231         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4376720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000231 DATLJT010000000
VERSION     DATLJT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4376720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1116
                     /locus_tag="VM910_05305"
     CDS             103..1116
                     /locus_tag="VM910_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI61997.1"
                     /translation="MNFPNTHYLRRPPTAVGNADVILGLELTDFWAIANAYIDNGEHG
                     VGVNTSRIKPETKLISINASELLTKANYQDFQRFQSVDVSMPADAEATLPALIEAVKA
                     AIPSDRKAAIEKRGEAARKAYAEQRERTKQAAALAWDASPISTARMSMEIWAHIKDLD
                     WSLVSVSGVGFNWPNRLWPMEKHYRWLGASGGYGVGYGAPASVGAALANRDLGRFSVN
                     IQADGDLMYAPGVLWTAARHNIPQLAVMFNNRGYHQEVMHVQRLSNFRNRVANLGNDM
                     GPIGTSIQNPDIEYHKLAESMGWWAKGPIKDPAQLGPALKEAVAVVKSGQPALLNVWT
                     QPR"
     gene            1133..1477
                     /locus_tag="VM910_05310"
     CDS             1133..1477
                     /locus_tag="VM910_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI61998.1"
                     /translation="MRLVRRFGPAALAAGLALGCGGAFAASAEKGKAAYIQHGCWQCH
                     GMQAQGGPGPKLAPDPIPIETFTAFVRTTNRAMPPYSEAILSNDDLADIHAYLQTIPK
                     PADYKSIPLLNQ"
     gene            complement(1658..>2045)
                     /locus_tag="VM910_05315"
     CDS             complement(1658..>2045)
                     /locus_tag="VM910_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI61999.1"
                     /translation="HLYCCGPTPMLKAFEAATADWPRAQIHVEYFTPKQEADKKGGFV
                     VELARSGQEFVIPEGKSILQVLLDAGVDVDYSCELGICGACEQRVISGIPEHRDAILT
                     EEEQASNTKVMICCAGCKSERLVLDL"
BASE COUNT          381 a          663 c          652 g          349 t
ORIGIN      
        1 gtgatcgtcg ccgatcgcgc cgctcgcagc gagaatggcg tccggctcct ggtcgagctg
       61 gccgagatcc tgcaggcgca ggtgatcgac cagggcggcc gaatgaactt ccccaacacg
      121 cattatctgc gccgtccgcc cacggcggtc ggcaacgccg acgtcatcct gggcctggag
      181 ctgaccgact tctgggcgat cgccaacgcc tatatcgaca acggcgaaca cggggtcggc
      241 gtcaacactt cgcggatcaa gcccgagacc aagctgatca gcatcaacgc gtcggagctg
      301 ctgaccaagg cgaactatca ggacttccag cgcttccagt ccgtcgacgt gtcaatgcct
      361 gccgatgccg aagcgacgct tcccgcgctg atcgaagcgg tgaaggcggc gattcccagc
      421 gaccgcaaag ccgccatcga gaaacgcggc gaagctgcaa ggaaggccta tgccgagcag
      481 cgcgagcgca ccaagcaggc ggcggcgctg gcctgggatg cgagcccgat tagtacggcg
      541 cgcatgtcga tggagatctg ggcgcacata aaggatctgg actggtcact ggtgtcggta
      601 tccggcgtcg gcttcaactg gcctaaccgg ttgtggccga tggagaagca ttatcgctgg
      661 ctgggggcat cgggaggata tggcgtgggg tacggtgctc ccgcctcggt cggtgccgcg
      721 ctcgcgaacc gcgatctcgg ccgcttctct gtcaacatcc aggccgacgg cgatctcatg
      781 tacgcaccgg gcgtgctgtg gaccgctgcg cggcacaaca ttccgcagtt ggccgtgatg
      841 ttcaacaacc gcggctatca ccaggaagtc atgcacgtgc agcgcctgtc caacttccgc
      901 aatcgcgtcg ctaaccttgg taacgacatg gggccaatcg gcaccagcat ccagaacccg
      961 gacatcgagt atcacaagct cgcagaatcc atgggctggt gggcgaaggg gccgatcaag
     1021 gatccggcgc agctcgggcc ggcgctcaag gaagcggtcg cggtggtgaa atctggccag
     1081 cccgcgctgc tcaacgtctg gacgcagccg cggtagggag ggaggcctgt cgatgcgttt
     1141 ggttcgtcgt tttgggccgg cggcactcgc cgccggcctc gcactcggct gcggcggcgc
     1201 attcgctgcc tcggccgaga agggtaaggc ggcttacatc cagcacggct gctggcagtg
     1261 ccacggcatg caggcgcaag gcggtcctgg ccccaagctc gcgccggatc ccatcccgat
     1321 cgagacgttc acggccttcg tgcgcaccac caaccgcgcc atgccgccct actcggaggc
     1381 gatcctgtcg aacgacgatc tcgcggatat ccacgcctat ctgcagacca tcccgaagcc
     1441 ggcggattac aagagcattc ccctgctcaa ccagtagtgc gagcagaata gcgtccgcat
     1501 aacaaaactc cgttcattcc cgcgcatagc gcgtcgttaa gggcgttcac gcccgtcttc
     1561 gacgggttat ggacgcgcgt aaacgcgctt atggcgggaa tccagggcca aaagctgggt
     1621 ccccgctttc gcggggacga gcggccagag gtccggatca cagatcgagc accagccgct
     1681 cgctcttgca gccggcgcaa cagatcatga ctttggtgtt cgaggcctgc tcctcctcgg
     1741 tcaggattgc gtcgcggtgc tcggggatgc ccgaaattac ccgctgctca caggctccgc
     1801 agatgccgag ctcgcaggaa tagtcgacgt ccacgccggc atcgagcagc acctgcagga
     1861 tgctcttgcc ttccggaatg acgaattcct gtcccgagcg cgcgagctcg accacgaagc
     1921 cgcccttctt gtccgcctcc tgcttggggg tgaaatattc gacgtggatc tgcgcgcgcg
     1981 gccaatccgc ggtcgcggcc tcgaacgcct tgagcatcgg cgtcggtccg cagcaataga
     2041 ggtgc
//
LOCUS       DATLJT010000232         7749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2768938,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000232 DATLJT010000000
VERSION     DATLJT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768938"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VM910_05320"
     CDS             complement(<1..237)
                     /locus_tag="VM910_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI62000.1"
                     /translation="MEHYVGLDVSLKLTAICIVDRTGKIEREGVVASDPETIAAFIKS
                     HAPHVARVGLEAGATSTWLWTELNKLGLPVICIDA"
     gene            717..1535
                     /locus_tag="VM910_05325"
     CDS             717..1535
                     /locus_tag="VM910_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62001.1"
                     /translation="MSIADTASRIAAPATAQMSIDGVRKRFGGVLAIDDVSVEFPRGS
                     ILSIIGPNGAGKTSLLNMISGFYKPDAGRIMLENRDITRQRPSSIAALGVARTFQNIA
                     LFGGLSVLDNLMLGRHARMRSGVLGCVLYWGLAQREEIRHREICEEIIEFLKLQDLRK
                     QPAAALPYGLRKRVELGRALALEPKVLLLDEPMGGMNQDEKEDIARYILDVNQERGIT
                     IVLIEHDMGVVMDLSDHVVVLERGRKIAEGAPVDVQNDPAVIAAYLGTSKAVRS"
     gene            1616..3475
                     /locus_tag="VM910_05330"
     CDS             1616..3475
                     /locus_tag="VM910_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI62002.1"
                     /translation="MREKDRGIWQSYSWRQCYDHVREFALGLAAAGFKRGDKLSVLGD
                     NRPRLYWAQLAAQALGGMAVPLYQDSIASELAFVLQHAEVVMIVAEDQEQIDKILSIA
                     GELPSLRLLVYEDSRGMSAYDHPLLKSFTDLEAMGRAFDAIQPGYFERELNEGRGSDV
                     ALIAYTSGTTGRSKGALLSHVNMIATSESFASVESIERGDNWLCYLPMGWVGDSMFSL
                     STSLVVAATCNCPESPETVQRDLRELGPSALLGPPRIWESMLTGLQVRAADASWLKRH
                     VYEYFRRIAQRRELLKGDSKPIPFSLALAYRVGEALVFGPVRDQLGLRNARWCLTAGA
                     PLGADTFRFFRSFGINLKQIYGVTEASALVAMQGDDEADPNTVGRPLPGTDVRIGELG
                     EVQVRGPGVFVGYYKQEDATRDAITPDGWLKTGDAGLVDPRGQLAIIDRVRDVGKLAD
                     STPFAPQFIENKLKFSPYVREAVAFGDRRSFVTAMIAIDISTVGKWAEQRSLPYTSYM
                     DLARKPEVASLVIEEVAKINATLPEPTKIRRVVLLNKELEADDAEMTRTRKVRRAFVA
                     EKYAPVIEALYSGAGVTQLTMNITFEDGRRSSLTTTLAVHDVLSCDAAGRVAA"
     gene            3497..4393
                     /locus_tag="VM910_05335"
     CDS             3497..4393
                     /locus_tag="VM910_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62003.1"
                     /translation="MWDELEFAFALSVSGLSIGLMYSLIALGFVLVYKATEAINFAQG
                     EFVMLAGFIVALILAVKGAPVIVAVGAVLALMILFSFGLERIVLRPLLGRPVIAVIMA
                     TIGLGAILRGLPPITVGGETRAVTLGIGDAPIAIGPASLPPIQVLGVVVTLAFLAIFT
                     WFFLRSRMGVAMRAVADNQQVAMAMGINVERYFALAWAMAGIVSALGGVIWGSMLGVD
                     VHLALVGLKVFPVVILGGLDSVVGAVVGGLIIGLVESLAAGYLDPYVGGGTKDFAPYV
                     VMIIALMFRPYGIFGTRRIERV"
     gene            4398..5465
                     /locus_tag="VM910_05340"
     CDS             4398..5465
                     /locus_tag="VM910_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62004.1"
                     /translation="MLHRESGVFKTSYAADMALYPLPIARWTVAAIVLLFTVVLPLSL
                     HEYYLSVINLALIAVVGALGLNILVGYTGQISVGHAAFMSVGAYTAANLAVHLHLPFW
                     VTLPAGGLMAALIGAIVGIPSLRIKGLYLAIATLAAQLIIEWVINHTPAISGGAQASI
                     EVPRPTLLGYQIKTQGQLYYFLLFFAALATLATLNLVRSRIGRAFVAIRDQDIAAEII
                     GINIFRYKLLSFAISSFYAGVCGVLYTYYFGIANYEAFQIGVSIDYLAMIIIGGFGSV
                     LGSIFGAVFVTMLPIAIRWIMEPLAGLFFGSGELSSIVASTRLVLFGCLIIFFLVVEP
                     EGLNRLWRNVRNYFRTWPFSY"
     gene            5503..6744
                     /locus_tag="VM910_05345"
     CDS             5503..6744
                     /locus_tag="VM910_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62005.1"
                     /translation="MSRSSNWRMTAIAAGAVALMAGNAAAQDKELVFGLQCDRTGATQ
                     TVGVFLCPGYHDYISLVNSKGGIEGYKIRVLEIDHEYKVPPAMEAHERFKKEGAVLEG
                     LYGTPQTAALTKKLEEDKILGTSPGFGTAAASDGKRYPYVFPIAASYWSQGAAAVAFA
                     KKELGGNLKGKKIAYLFYDNPAGKEPLVILEDLAKSEGFDLRTFAVPAPGVEMAAQVL
                     DITGRYRPDFVITHLFGRSPSVSIRELKSKGYPLSKVVSFVWGASEADIKAAGGYEVA
                     NGYHTIQFAGVGLDFQVIKDINAMYQAQGKPAPTAQEVSVFYNRGVMIAAIHTEAARA
                     AIKAKGGTKPTSEDIKKGMESIKGFTLGGMVPPMEITELDHEGGGWVQVWTVKDGKLV
                     KNGRWFQAYRDVINKHLAATN"
     gene            6809..7603
                     /locus_tag="VM910_05350"
     CDS             6809..7603
                     /locus_tag="VM910_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62006.1"
                     /translation="MLSVNNIEVVYDNVVLVLRGVSLEVRAGAITTLLGANGAGKTTT
                     LKAISGVLGTERGAVTKGSITFGDKRIDGMRPFDVTRIGISQVFEGRRVFEHLTTEEN
                     LIAGGHVQREPRKLRRGIELVYSYFPLLKERRHQLSGYLSGGEQQMLAIGRALMSEPK
                     VVLLDEPSLGLAPMLVVEIFDIVQRLVTQESLSVLLVEQNAAMAVSVAQHGYVMETGR
                     IVLEGSVQKLRDNSDIREFYLGLNEVGSRKSYRDVKHYKRRKRWLS"
BASE COUNT         1406 a         2432 c         2399 g         1512 t
ORIGIN      
        1 ggcatcgata caaatgacgg gcaatcccag cttattcaac tcggtccata gccaggtcga
       61 cgttgctccc gcctcaagcc cgactcgcgc gacatgcggc gcatgcgact tgataaatgc
      121 cgcgatggtc tccgggtcgg atgcaaccac cccttcgcgc tcgatctttc ctgttcgatc
      181 gacgatgcag atcgctgtca gcttcagcga tacgtccagt ccaacgtagt gttccattgc
      241 gtctctccat gattgcaagg ccccatgagg tgagccttgt tcgattcctg gagagctgat
      301 tccccaagcc ggagtatggc ttgagcccgg aattacccca tgtgtcaaaa ctccagcgct
      361 cgccgcagaa tcgctccgaa attttctacg cgcttgagac gctttggggt ttttacacgg
      421 ccaagaccct aagcagacat acggccgtcg attgaagccg cggagaccgg agatcgcgtg
      481 tagctcgccg aaaaacggca ttgcctaccg ccgaggcagg cgctcacggc cttttagggc
      541 gtggcgaggg ccatggtgct caactatcat tccaccgaca ccgcgcgcat tctcgccgaa
      601 agagcggtag ccgccgagtg gcaattgggg gaacaggcta tggcaatcgg caggctccag
      661 cgccaatgcg agcatgcatc cgccttggcc tcctcagctc gttcggggac ggccacgtga
      721 gcattgccga cacagcaagc cggattgcag cgccagccac cgcgcagatg tcgattgacg
      781 gtgttcgcaa gaggttcggc ggggtgctgg ccatcgatga tgtgtcggtc gaatttcctc
      841 ggggaagcat cctgtcgatt atcggcccca atggcgcggg caagacctca ctgcttaaca
      901 tgatttccgg cttctacaaa ccggacgccg ggcggatcat gctggagaac cgcgacatca
      961 ccagacagcg gccgagcagt attgccgcgc tcggcgtggc gcgcaccttc caaaatatcg
     1021 ctctcttcgg cggcctcagc gtgctcgaca atcttatgct cggccgccac gcgcgcatgc
     1081 gctccggcgt gcttggttgc gtgctctact ggggtctggc tcagcgcgag gaaatccgcc
     1141 atcgcgaaat ctgcgaggag atcatcgaat tcctgaaact gcaggatctg cgtaagcagc
     1201 cagctgccgc gcttccgtat ggattgcgca agcgggtaga gctcgggcgt gcgctcgcgc
     1261 tcgagcccaa ggttctgctg cttgatgaac ccatgggggg catgaaccag gatgagaaag
     1321 aggacatcgc gcgctacatc ctcgacgtca accaggaacg cggcatcacg atcgtgctga
     1381 tcgagcacga tatgggtgtg gtgatggacc tttccgacca cgtcgtggtg ctagagcgtg
     1441 gccgcaagat cgccgagggt gcaccggttg acgtacagaa tgatccagca gtcattgccg
     1501 cgtatctcgg caccagcaag gcggtgcggt cttgaacctt gtcgagcacc cagccatgga
     1561 cactctgccc aagctgctgc agggcaatat gcagactttc ggcagccaag tcgggatgcg
     1621 cgaaaaggac cgcggcatct ggcagtccta ttcctggcgt caatgctacg accacgtgcg
     1681 ggagtttgcc cttggcctgg ccgcagccgg gttcaagcgt ggcgacaagc tctccgtgct
     1741 cggcgacaac cgtccccgtc tttactgggc gcagctggcc gcgcaggcgc tcggcggcat
     1801 ggccgtgccg ctctaccaag actcgattgc cagcgaactg gccttcgtgc tgcagcacgc
     1861 cgaggtcgtg atgatcgtcg ccgaagacca ggagcagatc gacaagatcc tctccatcgc
     1921 cggtgagttg cccagcctgc gtcttctcgt ctacgaagac tcccggggca tgagcgccta
     1981 cgaccaccct ctgctcaagt ccttcaccga tttggaggcg atggggcgcg ccttcgacgc
     2041 gatccagccc ggctacttcg agcgcgagct gaatgagggc cgcggtagcg acgtggcgct
     2101 gatcgcctac acctcgggta ccaccggccg ctcgaagggc gccctgttga gccatgtcaa
     2161 catgattgcc acgtcggaga gcttcgccag tgtcgagtcg atcgagcgcg gcgataactg
     2221 gctgtgctat ctgccgatgg gctgggtcgg cgactcgatg ttctcgctct ccaccagctt
     2281 ggtggtggcg gcgacctgca attgcccgga gagccccgag acggtgcagc gcgacctgcg
     2341 cgaattagga ccgagcgcgc tgctggggcc gccacgcatc tgggagagca tgctgactgg
     2401 cctgcaggtg cgagccgccg acgcctcctg gctgaagcgg cacgtctacg agtattttcg
     2461 ccgcatcgcc cagcggcgcg agctgttgaa gggtgacagc aagcccatcc cattctcgct
     2521 tgcgctcgcc tatcgggtgg gagaggcgtt ggtcttcggt cccgtgcgcg accaactcgg
     2581 gctcaggaat gcacgctggt gcctgaccgc cggagcccca ctgggtgccg acacattccg
     2641 cttcttccgc tcgttcggca tcaatttgaa gcagatctac ggcgtcaccg aagccagcgc
     2701 cctggtcgcc atgcagggcg acgacgaggc cgatcccaac accgtaggac gccccttgcc
     2761 cggcaccgac gtgcgcatcg gtgagctcgg cgaggtgcag gtgaggggcc ccggcgtgtt
     2821 cgtcggttat tacaagcagg aggatgcgac ccgggatgcc attacgccgg acggctggct
     2881 gaagacgggc gatgcaggcc tcgtcgatcc gcgtgggcaa ctcgccatca tcgatcgcgt
     2941 tagggacgtc ggcaagcttg ccgacagcac gccgttcgca ccgcaattca tcgagaacaa
     3001 gctcaagttc agcccctacg tccgcgaagc ggtggccttc ggggatcggc gctcattcgt
     3061 gacggcaatg atcgcgattg acatctcgac cgtcggcaag tgggccgagc aacggagcct
     3121 gccctatacc agctacatgg atttggcccg caagccggag gtggcgagcc tcgtcatcga
     3181 ggaggtggcc aagatcaacg ccacgctgcc cgaacccacc aagatccgcc gtgtggtcct
     3241 gctcaataag gagctggaag ccgacgatgc tgagatgacg cgcacgcgca aggtgcggcg
     3301 cgccttcgtc gcagagaaat atgcgccggt gatcgaggct ctctacagcg gcgccggggt
     3361 gactcagctc accatgaaca tcaccttcga ggacgggcgc aggagctcgc tgacgacgac
     3421 gctcgccgtt cacgatgtcc tttcttgcga cgctgccggc cgcgtggcgg cgtgaccggg
     3481 ctcgaagagg tagttgatgt gggatgaact cgagttcgcc tttgcgctca gcgtcagcgg
     3541 tctgtcgatt ggacttatgt attcgctgat cgcgctcgga ttcgtgctag tctacaaggc
     3601 cacagaggcg atcaatttcg cgcaaggcga gttcgtcatg ctggcgggct tcattgtggc
     3661 gctgatcctc gccgtcaagg gcgcccccgt tatcgtcgcc gtcggtgcgg tgctggcgct
     3721 catgatacta ttcagcttcg ggctggaacg gatcgtccta aggcccttgc tcggccggcc
     3781 agtcatcgcc gtgatcatgg ccaccatcgg cctcggcgcc atcctgcgcg gcttgccgcc
     3841 gatcacggtc ggcggcgaaa cgcgcgccgt aacgctcggc atcggcgacg caccgatcgc
     3901 gatcggaccg gcctcgctgc cgccgattca ggttctcggc gtggtcgtca cgctcgcgtt
     3961 cctcgctatc ttcacctggt tcttcctcag gagccggatg ggcgtggcga tgcgggcggt
     4021 ggccgataat cagcaggtgg ccatggccat gggcatcaat gtggagcgct atttcgcact
     4081 ggcctgggcg atggccggca tcgtctcggc actcggcggc gtgatctggg gcagcatgct
     4141 cggtgttgac gtgcacttgg cgttggtcgg cttgaaggtg tttcccgtgg tcatcctcgg
     4201 cggcctcgac tccgtggtcg gtgctgtcgt gggcggcctc atcatcggcc tggtcgagag
     4261 cctcgccgcc ggctacctcg atccctatgt gggcggcggc accaaggact tcgcgcccta
     4321 cgtggtcatg ataatcgccc tgatgttcag gccatatggc attttcggca cgcgtaggat
     4381 cgagcgggtg taacgctatg ctgcatcgcg agtccggagt gttcaagacc agctacgccg
     4441 ccgatatggc gctatacccg ctgccgatcg cgcgctggac ggtggcggcc atcgtccttc
     4501 tcttcaccgt tgttttgccg ctgtccctgc acgagtacta tctttccgtc atcaacctcg
     4561 ccctcatcgc cgtcgtcggc gcgctgggcc tcaatattct ggtcggctac accggccaga
     4621 tctcggttgg ccatgccgcc ttcatgtcgg tcggtgccta cacggccgcc aacctcgccg
     4681 tgcacctgca tctgccgttc tgggtgacgc tgcccgccgg cggattaatg gccgccttga
     4741 tcggcgccat cgttggcatc ccgagcctca ggatcaaagg cctctatctc gccatcgcca
     4801 ccctggcggc ccaactcatc atcgaatggg tcatcaacca cacgcctgcc atctctggtg
     4861 gggcgcaagc ctccatcgag gtgccgcggc cgacgctgct cggctatcag atcaagacac
     4921 aggggcaact ctattacttc ctgctgttct tcgctgccct cgccaccctt gccacgctca
     4981 acctcgtcag gagccggatc ggccgcgcct tcgtcgccat tcgcgaccag gacatcgccg
     5041 ccgagatcat cggcatcaac atcttccgct acaagctgct gtcgttcgcc atctcgtcgt
     5101 tctatgccgg cgtatgcggg gtgctctaca cttactactt cggcatcgcc aactacgagg
     5161 cattccagat cggcgtgtcg atcgactatc ttgccatgat catcatcggc gggttcggct
     5221 cggtactcgg ctccatattc ggggctgttt tcgtaaccat gctgccgatc gctatccgct
     5281 ggatcatgga gccgctggcc ggtctgtttt ttggctccgg cgagctttcc agcatcgtcg
     5341 ccagcaccag actggtcctt ttcggctgcc tcatcatctt ctttctcgtg gtcgagccgg
     5401 agggtctcaa ccggctatgg cgcaacgtcc gtaactactt ccgtacctgg ccattctcgt
     5461 attgaattgc tgcaagcaat cacctccgag ggaggaaact caatgtcgag aagctcaaac
     5521 tggcgcatga cggcgatcgc tgccggcgcc gtcgctctca tggcaggcaa cgctgcggcg
     5581 caggacaagg agctggtatt tggcctgcaa tgcgaccgca cgggcgccac ccagaccgtg
     5641 ggtgtgtttc tctgcccggg ctatcacgac tacatcagct tagtgaacag caagggcggc
     5701 atcgaaggtt acaagatccg cgtgctcgag atcgatcacg agtacaaggt gccgcctgcc
     5761 atggaggcgc acgagcgctt caagaaggag ggcgccgtgc tagagggcct ctatggcacg
     5821 ccacagacgg ccgcactgac caagaagctc gaggaagaca aaatcctcgg cacctcaccc
     5881 ggcttcggca ccgccgctgc gagcgacggt aaacgctatc cttatgtttt cccgatcgcg
     5941 gccagctact ggtcgcaagg tgcggcggcc gtagccttcg ccaagaagga attggggggc
     6001 aatcttaagg gcaagaagat cgcctacttg ttttatgaca acccggccgg caaggagccg
     6061 ctcgtcatcc tcgaggacct agccaagagc gaaggcttcg acctgcggac cttcgccgta
     6121 ccggcacccg gcgtcgagat ggccgcgcaa gtgctcgaca tcaccggtcg ttacaggccc
     6181 gatttcgtca tcactcatct gttcggacgt tccccctcgg tgtccatcag ggagctgaag
     6241 agcaagggtt atccgctgag caaggtggtg tcattcgtgt ggggcgcctc cgaagccgac
     6301 atcaaggcgg ccggcggtta cgaggtcgcc aacggttacc acacgatcca gtttgccggt
     6361 gtcggcctgg acttccaggt catcaaggac atcaatgcga tgtaccaggc ccagggcaag
     6421 ccggcaccga ccgctcagga ggtgagcgtc ttctacaatc gcggtgtcat gatcgccgcc
     6481 attcacaccg aggccgcgcg cgccgcgatc aaggccaaag gcggcaccaa gccgactagc
     6541 gaggacatca aaaaaggcat ggagtccatc aagggcttca cgctaggcgg catggtgccc
     6601 ccgatggaga tcaccgagct cgatcacgaa ggcggcggct gggtgcaggt ttggaccgtc
     6661 aaagatggca agctcgtcaa gaacggtcgc tggttccagg cttaccgcga cgtgatcaac
     6721 aagcatctcg cggcaaccaa ctagccgggg gcatcaggaa cggccggcga ggttctcgcc
     6781 ggtcgggcct ctccagcgac aaggtccgat gctatcggtc aacaacattg aagttgtcta
     6841 tgacaacgtc gtcctggtgc tgaggggcgt gtcgctcgag gtgcgcgcgg gcgccatcac
     6901 gacgctgctt ggggccaacg gcgccggcaa aaccaccact ctcaaggcca tctcgggtgt
     6961 gctcggcacg gagcgcggcg ccgtgaccaa gggctccata accttcggcg ataagcgcat
     7021 cgacggcatg cgaccgttcg acgtgacgcg catcggcatc tcacaggtgt tcgaaggccg
     7081 gcgcgtgttc gagcacctga ccaccgagga gaacctgatc gccggcggcc acgtgcagcg
     7141 cgagccgcgc aagctgcgcc gcgggattga gctcgtctat tcttactttc cattgctcaa
     7201 ggagcggcgt catcaactgt ccggatacct ctcaggcggc gagcagcaga tgttggccat
     7261 cggccgcgca ttgatgtctg aaccgaaagt cgtgctgttg gacgagcctt cgctgggcct
     7321 ggcgccgatg ctggtcgtag agatcttcga catcgttcag cggctggtca cgcaagagag
     7381 cctgtctgtg ctgttggtgg agcagaacgc cgcaatggcg gtttcggtgg cgcagcacgg
     7441 ctatgtcatg gagacgggcc gcattgtgct cgaaggcagc gtccagaagc tgcgcgacaa
     7501 ctccgacatc cgcgagtttt atctcggcct caatgaggtg ggctcgcgca agtcctaccg
     7561 tgatgtcaag cactacaaac ggcgcaagcg gtggctttca taggcgttgg ccgtttcgag
     7621 ccgcactgag cgcgaccgag acggtgcgca ctgtcccgat catgtattga cgatcggtcg
     7681 cgcaacggag gctttcacca cgggccacac gttgcgcggt cgcgccggca acggcgcgag
     7741 gatgccggc
//
LOCUS       DATLJT010000233         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2309297,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000233 DATLJT010000000
VERSION     DATLJT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309297"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1918)
                     /locus_tag="VM910_05355"
     CDS             complement(215..1918)
                     /locus_tag="VM910_05355"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVI62007.1"
                     /translation="MQNQSVTEPSAAPDPDAPITEQLHNLSNGKFDRIIGSTKERESI
                     DAFYASRNYAPLWITEGKANARAKAVIAYLGGVAADGLDPTDYPVPNFASLTDGAALA
                     EAEISLTASVIAYAHHAQIGRVHWSRVSGDIFYDRSAPDPGDVLATMAQAKDAGEALD
                     AYEPHAEAYRALKAKLAEVRPSQGGSDKPRISNGRSLKIGMRDERVPLLRLRLGVASQ
                     EATYDKELADTVRKFQERRGLKPTGTLDPATVEALNGRQPDRQIDIIIANMERWRWMP
                     HDLGNTYVIVNLPDYTLRVIHQGKQVWMTKIVAGKPATPTPIMSAQMKSITVNPTWNV
                     PDSIASSEYLPLLQQDPTILQRMGLQVSYNPDGSIHLSQPPGEQNALGQIRFNFPNKF
                     LVYQHDSNQKYLFANEKRADSHGCMRVQDPVKYAEVLLSMVRPGEGYTQDRIRRMFGA
                     YEADIQFPTFIPVHLTYQTAFVDDQGKLEFREDIYGRDQALLAILNGTERKVADVPIQ
                     RKDSTGRRQLLAMPDRYWGGWGRGFAGGSNFFTQLFGGPSVRPAPVPRRPVAQYRAFY
                     Q"
     gene            complement(2170..>2796)
                     /locus_tag="VM910_05360"
     CDS             complement(2170..>2796)
                     /locus_tag="VM910_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62008.1"
                     /translation="FRSRQAWDYGHTFVVFGRVGEAPSKHNVAGLSPKGDDPSMWVMG
                     HYVPVPSDTGWTDGDLEDRYITARYRVLVSKEQYDRTVAYIRQLQAKSTTWSVEMYNC
                     NAFVADIANFMGLKAPSSTWIYPRVFVTNMRKINTGHPEAANELVSENVKEMSNPTRD
                     GRAMISAGMIKSDGAPTGGSASPPHVTIGAVRVSNRPASSTGSAQHSP"
BASE COUNT          518 a          802 c          879 g          597 t
ORIGIN      
        1 tgagaggaac cttgatctga atcaagaatt tccgcgtacc cgctgtggtt atgaacgagc
       61 gcgggtcaat gtccgctttg ggtcaaagca gacttgggag tgtgtctgcg taatgtccgc
      121 tttatctccg aaagcggaca ttgactcatc cgcttatgag gacacgcttt cccttgtcga
      181 ctagggccag tcgatcatcg ccgatccggt cgcgtcactg ataaaacgcc cggtattgcg
      241 ccacgggccg gcgcgggact ggcgccggtc ggacggacgg gccaccgaac aattgcgtga
      301 agaagttcga cccgccggcg aagccgcgcc cccagccgcc ccagtaacgg tcaggcattg
      361 ccaggagctg tcgacgcccc gtgctgtcct tgcgctggat gggaacatca gcaactttgc
      421 gttcggtccc attcagtatg gcaagcagcg cctggtcgcg gccgtaaata tcctcgcgga
      481 attcaagctt gccctggtca tcgacgaaag cggtctggta ggtcaggtgg accgggatga
      541 aggttggaaa ctgtatgtcg gcctcgtagg cgccaaacat ccttcggatg cggtcctgag
      601 tatagccttc gccgggccgc accatcgaca acagcacctc tgcatatttc accggatcct
      661 gtacccgcat gcagccgtgg ctgtcggccc gcttctcatt tgcaaataga tatttttggt
      721 tcgagtcgtg ttggtagacc aggaacttgt tgggaaaatt gaagcggatc tggccgagcg
      781 cgttttgctc acccggcggc tgggacaggt gaatgctgcc atccgggttg tagctgactt
      841 gcaatcccat ccgctgcagt atcgtcgggt cctgctgtag caaaggcaga tattcactag
      901 aggcgatcga gtccggtaca ttccaagtcg gattgacagt gatcgacttc atctgcgcgc
      961 tcatgatcgg cgtcggcgtc gccggcttgc cggccacgat cttggtcatc cagacctgct
     1021 tgccctggtg gatcacgcgc agcgtgtagt cgggaaggtt gacgatgacg taggtgttgc
     1081 caagatcgtg cggcatccac cgccagcgtt ccatgttggc gataatgatg tcgatctgcc
     1141 gatcgggctg gcggccgtta agcgcttcga ctgtggccgg atcgagggtg ccggtcggct
     1201 tgagtccacg cctttcctgg aacttcctca cggtatcggc aagttccttg tcgtaggtcg
     1261 cttcctggct cgcaacacca agccgcaagc gcaggagcgg cacccgctcg tcgcgcatgc
     1321 caattttgag cgaccgcccg ttcgaaatgc gaggcttgtc ggagccgccc tggctggggc
     1381 gaacttcggc cagcttggcc ttgagcgcgc ggtaggcctc ggcgtgcggc tcgtaggcgt
     1441 cgagcgcctc gcccgcatcc ttggcttgcg ccatcgtggc gagaacgtcg ccagggtccg
     1501 gtgcgctccg gtcataaaag atgtcgccgc tcacgcgcga ccagtggacg cggccgattt
     1561 gcgcatgatg cgcataagcg ataaccgaag ccgtgagact gatctcggct tctgcgagcg
     1621 cggctccatc agtgagcgaa gcaaaattcg gaacggggta gtcggttgga tcaagaccat
     1681 cggccgcgac accgccaaga taggcgatca cggccttggc gcgcgcattg gccttccctt
     1741 cggtgatcca tagcggcgca taattgcggc tggcgtagaa agcgtcgatg ctttctcttt
     1801 ctttcgtgct gccgataatg cggtcgaatt tgccgttgga tagattgtgc aactgttcgg
     1861 tgattggcgc atcgggatca ggagcagcgc tcggctcggt cacggattgg ttctgcacac
     1921 taggcgccac gattgcggta gtggacggtc cggacgtatc gctagccgca ctcgccggcg
     1981 agcccacaat ctccgcattc gacgcgggct tattggcatc ggcacttgcg ccggtcgaat
     2041 ccgcaagggc agcgcgcgga ttcaacagca gtaagacaag tggagttaag gcgataagcc
     2101 gatcgaggcg cagacgcggc attgcaaatc ccccactagg gcgcgcgaaa gatcctgacg
     2161 ctggttcgct taaggcgagt gttgtgcact gccagttgaa ctcgccggtc ggttagagac
     2221 gcgaacggca ccgatggtga cgtggggcgg cgacgccgag ccacccgttg gcgcgccgtc
     2281 cgacttaatc atgccggcgg agatcatggc gcggccgtcg cgggtcggat tgctcatctc
     2341 ctttacattc tcactgacga gctcgttggc ggcttccgga tgtccggtat tgatcttgcg
     2401 catattggtc acgaagactc tcggatagat ccaggttgag gagggcgcct taagacccat
     2461 gaagtttgcg atgtcagcga cgaaggcgtt gcagttgtac atctccacgc tccaggtcgt
     2521 cgatttggcc tgtagctggc gaatgtaagc aacggttctg tcgtattgct ctttgctcac
     2581 cagaacgcgg tagcgcgcgg tgatgtatct gtcctcgagg tcaccgtcgg tccagccggt
     2641 atcggagggg accggaacat aatgccccat gacccacatc gaggggtcat cgccctttgg
     2701 cgaaagcccg gctacgttgt gcttgctcgg cgcctcgccg acacggccga agaccacgaa
     2761 ggtgtggccg tagtcccaag cctggcgtga gcggaa
//
LOCUS       DATLJT010000234         2733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_11722,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000234 DATLJT010000000
VERSION     DATLJT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11722"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..398)
                     /locus_tag="VM910_05365"
     CDS             complement(207..398)
                     /locus_tag="VM910_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HVI62009.1"
                     /translation="MPVEDDDRPRKKVSHEIGQDLSLLSVEELNERIALLSSEVERIK
                     EVVAKKRASRDAANSVFKS"
     gene            493..1491
                     /locus_tag="VM910_05370"
     CDS             493..1491
                     /locus_tag="VM910_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HVI62010.1"
                     /translation="MEKLPAQMTVIGISKPGGPEVLVPETRAVPRPGPDEILIKVLAA
                     GVNRPDVSQRQGHYPPPPGASDLPGLEVAGEVVAVGSNAKKHKPGDKVMSLVAGGGYA
                     QYCIAQDAQAMAVPPSLSIAEAGAIPETLMTVWHNVFERGALKAGETLLIHGGSSGIG
                     TMAIQLAKAFGAKVIVTVGSKEKADACLKLGADRAINYKTEDFVAEVKAATSGAGANV
                     ILDMVGGDYIERNYAAAAVDGRVVQIAFLGGPKATVNFTGLMVKRLTHTGSTLRPRSN
                     ADKAAMVAAIEAKVMPLLREGRIKPLMDSSFPLEKAADAHRRMEASEHIGKIVLLA"
     gene            1682..>2733
                     /locus_tag="VM910_05375"
     CDS             1682..>2733
                     /locus_tag="VM910_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HVI62011.1"
                     /translation="MRAIRYLAPLSLVLGLMMFVAALPANALDAVSVRGDAPAIDLTG
                     VLEHQRSESDRIQVSTAPGTDGIVRRIEVRAREGGQNWVVFALANNTDDQLDRLIVAP
                     HYRIVASGLLWPDLGLSRIATITPSTGDRPDRQESLTADVFRITLDPGAVITFVAELR
                     TDKLPQLYLWEPDAYKDKVNSFTLYQGIVIGISGLLALVLTILFVVKGSMMFPAAAAL
                     AWAVLVYIGVDFGFWGKVLDMSNNAERIWRAAGEAILAATLLVFLFAYLNLSRWHVRY
                     SHITIGWLTFLGSLVALALFDPAVASGIARMSLVLIAFAGFALIVYLSTHGFDRAVLL
                     IPTWFLLVIWVIAAGVT"
BASE COUNT          476 a          880 c          844 g          533 t
ORIGIN      
        1 gtttccggcg gcttttgaag ttgataacag ggaggcgtca aacagagtgg acaggagcca
       61 ctcggtgtcc aaacgaggac aatgacagtc ataaaggcga aagcttaatc gatcgtaaac
      121 gcgcgaattt tttgagagat cattagccat gtcggccatt ggccgagttt cggggtcaag
      181 cagcccgctt tcttcccgcg cgccgtctac gacttgaaga cgctgttggc ggcatcgcgc
      241 gaggcgcgct ttttggcgac gacttccttg atccgttcga cttcggacga caacagcgcg
      301 atccgctcgt tgagctcctc gaccgacagc agcgacaggt cctgaccgat ctcgtggctc
      361 accttcttgc gcggccggtc gtcgtcttcc acgggcatcg cttgcctccc tcgttctgcc
      421 gattgccagc ggcgagccgg ctggttaatc aggtccggct tcgacgctgc aatcgaacca
      481 ggacaaatca ccatggaaaa gctgcctgcg caaatgaccg tcatcggcat cagcaagccc
      541 ggcggccccg aggttcttgt tcccgagacg cgggcggtcc ccaggccggg cccggacgaa
      601 atcctgatca aggttttggc cgcaggcgtc aatcgccccg acgtctcgca gcgtcagggc
      661 cactacccgc cgccgcccgg cgccagcgac ctgcccgggc tcgaggtcgc gggcgaagtc
      721 gtggcggttg gaagcaatgc aaaaaaacac aagcccggcg ataaagtgat gtcgctggtc
      781 gcgggcggcg gctacgcaca atattgcatc gcccaggacg cccaggcgat ggcggtgccg
      841 ccgtcgctct cgattgcgga agcgggcgcg atcccggaaa cgctgatgac cgtctggcac
      901 aatgtgttcg aacgcggcgc gctgaaggcg ggcgagacgc ttttgatcca tggcggctca
      961 tccggaatcg gcaccatggc gatccagctt gccaaggcgt tcggggcgaa ggtgatcgtc
     1021 accgtcggct caaaggagaa ggccgacgcc tgcctcaagc tcggggcgga ccgcgctatc
     1081 aactacaaga ccgaggattt cgtcgccgag gtcaaggccg caacctccgg cgccggcgcc
     1141 aatgtcatcc tcgacatggt cggcggcgac tatatcgagc gcaattacgc ggcggccgca
     1201 gtcgacggcc gcgtggtgca gatcgccttc ctgggcggtc ccaaggcgac cgtcaatttc
     1261 acggggttga tggtcaagcg cctgacccac accggctcga cactgcgtcc ccgtagtaat
     1321 gcggacaaag ccgccatggt cgccgcgatc gaggcgaagg tgatgccgtt gctgcgcgag
     1381 gggcggataa agcccttgat ggacagcagc ttccccctgg aaaaggcggc cgatgcgcac
     1441 cggcgcatgg aggcctccga acatattggc aaaattgtgt tgctggctta atgctcgcga
     1501 ccaacaaaat tgtgttgctg gcttaacgct cgcaaccgat acgcgtgcga aaccctttga
     1561 tttctctcgc tttcatggca aagatcattc aacaaacgaa cgcatcgggc ggggtccccg
     1621 gatgcgtctt tcgctgcaac tcgtttcatt tcggcagagc gtcgagcgcg gagaattgac
     1681 attgcgtgcg atcaggtacc ttgcgccgct ttcgctggtg ctgggcctca tgatgtttgt
     1741 cgccgctttg ccggcgaatg cgctcgacgc cgtcagcgtc cgcggtgacg cgcccgccat
     1801 cgacctcacc ggcgtgctcg agcaccagcg cagcgagtcc gaccgcatcc aggtctcgac
     1861 cgcgccaggc accgacggca tcgtccgccg gatcgaggtg cgcgcccgcg agggcggcca
     1921 gaactgggtg gtgttcgcac tcgccaacaa caccgacgac cagctcgatc gcctgatcgt
     1981 cgcgccgcat taccgcatcg tcgcttcggg tctgctgtgg cccgatctcg gcctgtcgcg
     2041 cattgcaacg atcacgccgt cgaccggcga ccgtcccgat cgccaggaga gcctgaccgc
     2101 cgacgtcttc cgcatcacgc tcgatcccgg cgcggtgatc acctttgtcg cggaattgcg
     2161 caccgacaag ctgccgcagc tctatctctg ggagcccgac gcctacaagg acaaggtcaa
     2221 ctcgttcacg ctgtaccagg gaatcgtgat cggcatctcc ggacttcttg cgctcgtgct
     2281 cacgatcctg ttcgtggtga agggcagcat gatgtttccg gccgccgcag ccctcgcctg
     2341 ggcggtgctg gtctatatcg gcgtcgactt cggtttctgg ggcaaggtgc tagacatgtc
     2401 gaacaacgcc gagcgcatct ggcgcgcggc gggcgaggcg atcctcgcgg cgacattgct
     2461 ggtgttcctg ttcgcctatc tcaacctctc gcgctggcac gtgcgctatt cccacatcac
     2521 catcggctgg ctgaccttcc tcggttcgct ggtggcgctt gcgctgttcg accccgcggt
     2581 tgcctccggc atcgcccgca tgtcgctggt cttgatcgcc ttcgcgggct ttgcgctgat
     2641 cgtctatctc tcgacccacg gctttgaccg cgcggtgctg ctgatcccga cctggttcct
     2701 cctggtgatc tgggtgatcg cagccggggt gac
//
LOCUS       DATLJT010000235         3749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3917506,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000235 DATLJT010000000
VERSION     DATLJT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917506"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VM910_05380"
     CDS             complement(<1..644)
                     /locus_tag="VM910_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HVI62012.1"
                     /translation="MSDLDRNYNSPFGRVAGRADAATVDAGLRAYMLRIYNYMSIGLA
                     ITGLAALGVYMAAVTTDQASAAARFGNILLTPFGYAMFVSPLKWLFILAPLAMVFVIS
                     AGINRLAPSTAQLLFWVFSALMGISLSSIFLVYTHTSIVRVFFITAATFGALSLYGYT
                     TRRDMSGMGSFLFMGLIGIIIASLVNLFLVSSALQFVVSVIGVLVFAGLTAWDT"
     gene            complement(976..3567)
                     /locus_tag="VM910_05385"
     CDS             complement(976..3567)
                     /locus_tag="VM910_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62013.1"
                     /translation="MSVASEALLGVATEPAGGGRLWLSLRYALRDLRGGLRGFYVFIA
                     CIALGVMAIAGVGSVAASLSDGLAREGRNLLGGDVAFVLFQREAKPAELAFLRSRGQL
                     SVIATMRGMARSSDGRLALVEMKAVDGGYPMLGELSLDPALPLQDVLAERDGSFGAAA
                     DPALLARLDLKIGDKVTIGDTSFQIRSEVNAEPDKLSGGVGFGPRFLISDEGLRATAL
                     LQPGSLVRWSYRVRLPDNAADDRAVSALIDDARRAAPEAGWEVRSRSNASPQLERTIS
                     RFTQFLTLVGLAALLVGGVGVANAVKSHIDRRLDVIATFKALGATGRDVFSIYLTQVI
                     LLAALGSVIGLAAGAALPFVIVGLFGKLLPLPVVPALHASELALSFVYGLLTALAFGL
                     WPLGRVHDVPVAALFRDTVSTEWHRPRWRYLALMALVIAVLIGVVIGLAYDKRVAAVF
                     VVSAMIVFAVLRGIAGALMWLARTLPRTRFPMLRLAISNIHRPGALTPSVVLSLGLGL
                     AVLVTITQIDGNLRRQFLAALPERAPSFYFIDIPSSEADRFAAFLQKTEPQSTVEDVP
                     MLRGRIVAARGVRAEELTPSTDSEWVLQSDRGLTYTGEIPKGSKIVEGEWWGADYSGP
                     PLVSIEKKIADGLRLKLGDEIVVNVLGRDIHAHIGNMRNIDWQGLGINFVLVFSPNAF
                     KGAPHTHIATLTEPHPDTSGDVRIIKQVADAFPMVTSVRVREVMETIGSVVTNLVLAI
                     RGASAVTLISAILVLGGALAAGHRHRVYDAVILKTLGATRARLLAAYALEYLLIGLAT
                     AVFGVIAGSVAAYLIVTRLMTLSFVWEASSAAGVVLAALIVTVGLGLVGTLLALNQKP
                     ASVLRNL"
     gene            complement(3564..>3749)
                     /locus_tag="VM910_05390"
     CDS             complement(3564..>3749)
                     /locus_tag="VM910_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62014.1"
                     /translation="GNLDEATGKQIIDLLFTKHAERGMTLVLVTHDASLAQRCDRVVR
                     LRSGRVEPGGADRKVAS"
BASE COUNT          747 a         1227 c         1205 g          570 t
ORIGIN      
        1 tgggtgtccc aggcggtgag gcccgcgaac accagcacgc cgatcacgga caccacaaac
       61 tgcagcgccg aagagaccag gaacaggttg accaggctcg cgatgatgat gccgatcagg
      121 cccatgaaca ggaacgatcc cattccgctc atgtcgcgcc tggtggtgta gccgtacagg
      181 ctgagcgcac cgaaggtcgc cgcggtgatg aagaacaccc gcacgatcga ggtgtgggtg
      241 tacaccagga agatcgacga cagcgaaatg cccatcagcg ccgagaacac ccagaacagg
      301 agctgggcgg ttgacggcgc aaggcggttg atgccggccg aaatcacgaa caccatggca
      361 agcggtgcca ggatgaacag ccatttcagc gggctcacaa acatcgcgta gccgaacggc
      421 gtcaggagaa tgttcccgaa gcgcgcggcg gcactcgcct gatcggtcgt taccgcggcc
      481 atgtagacgc cgagtgcggc aaggccggta atggcgagcc cgatgctcat gtagttgtag
      541 atgcgcagca tgtaggcgcg cagcccggca tcgacggtcg cggcgtcagc gcgcccggcg
      601 acccggccga atggagagtt atagttgcgg tcaaggtccg acatggtcga attcccgttg
      661 gttgcccggc gggagcgcgc tgtaagcttc gtgtaagctt tgcgcctgcc ggttgatgaa
      721 acctatctca gttgccgaga cttgctgaca ttaaatttgc gtcatcaatc gcgccatctg
      781 atcagacttc tatttggcat ttccgtacaa aactaccata gcggcgagcg gccgccacgc
      841 gcggccgaat gtcgctctcg gccgtactcc cgtcatgcgc gaaccccaaa ttccataaag
      901 aatataaatt ccataaaaat cgcggggctc ccccgccggc ctcaaccctt cggccgacgg
      961 tggtgcttca atttgtcata gattccgcag caccgaagcc ggcttctggt tcagcgcgag
     1021 aagggtgccg accagtccaa gccccacggt gacgatcaag gcggccagca ccacgccggc
     1081 agcgctcgag gcctcccaga cgaagctcag cgtcatcagc cgcgtcacga tcaggtaggc
     1141 cgcgaccgag cccgcgatca cgccgaagac cgcggtggca agaccgatca agagatattc
     1201 cagcgcgtag gccgcgagca gccgcgcgcg cgttgcgccg agcgtcttca gaatgacggc
     1261 gtcatagacc cggtggcgat gaccggccgc gagcgctcca cccaacacca gaatggccga
     1321 gatcaaggtc acggcgctgg cgccgcggat cgccaacacc agattcgtca ccacggatcc
     1381 gatggtctcc atcacctcgc gcacgcggac gctcgtcacc atcgggaagg catccgccac
     1441 ctgcttgatg atacggacat cgccgctcgt gtccgggtgc ggctcggtca gggtcgcgat
     1501 atgggtgtgc ggcgcgccct tgaaggcatt gggcgagaac accagcacga aattgatacc
     1561 gagcccctgc caatcgatgt tgcgcatgtt tccgatatgc gcgtggatgt cgcggccgag
     1621 cacgttgacg acaatctcgt cgccaagctt cagccgcaag ccgtctgcga tcttcttttc
     1681 gatcgagacc agcggcggcc cgctatagtc ggcgccccac cactcgcctt cgacgatctt
     1741 ggagcctttc gggatttcgc cggtataggt cagcccgcgg tcgctctgca gcacccattc
     1801 ggaatcggtc gatggggtta gctcttccgc gcgcaccccg cgcgcggcga cgatgcgtcc
     1861 gcgcagcatc ggcacgtcct cgacggtcga ttgcggttcg gtcttctgca ggaacgcggc
     1921 gaagcgatcg gcttcggagg agggaatgtc gatgaagtag aatgacggcg cgcgctccgg
     1981 cagcgccgca aggaactgcc ggcgcagatt gccgtcgatc tgggtgatcg tgaccaaaac
     2041 cgcaagcccg agcccgagcg acagcacgac ggacggcgtc agcgcacccg gccggtgaat
     2101 gttggagatg gcaagtcgca gcatggggaa gcgcgtgcgc ggcaacgttc gtgccagcca
     2161 catcagcgca cccgcgatgc cgcgcaatac cgcgaacacg atcatcgcgg agacgacgaa
     2221 caccgccgcc acgcgcttgt cataggcaag cccgatcacc acgccgatca acacggcaat
     2281 gaccagcgcc atcagcgcga gatagcgcca gcgcggccgg tgccattcgg tgctcaccgt
     2341 gtcgcggaac agggctgcca ccggcacgtc atgaacgcgg ccgagcggcc atagtccgaa
     2401 ggcgagcgcg gtgagaaggc cgtagacgaa cgacagcgcg agctcgctcg cgtgcagtgc
     2461 cggcactacc ggtagcggca gcagtttgcc gaacaggccg acgatgacga agggcagcgc
     2521 cgcgccggcg gcaagtccga tcaccgagcc gagggccgcc agcaggatca cctgcgtcag
     2581 atagatcgag aacacgtcgc ggccggttgc gccaagcgcc ttgaatgtcg cgatcacgtc
     2641 gagacgccga tcgatgtggc tcttgacggc gttcgcgacg ccgaccccgc cgaccaacag
     2701 cgctgcgagg ccgaccaatg tcaggaactg ggtgaagcgg ctgatggtgc gctcgagctg
     2761 cggcgatgcg ttggagcggc tgcgcacttc ccaaccggcc tcgggcgctg cccgacgggc
     2821 gtcatcgatc agcgcggata cggcgcgatc gtcggccgca ttgtcgggca gtctgacgcg
     2881 atagctccag cgcaccaggc tgcccggctg caacagcgcg gtggcgcgca ggccctcatc
     2941 gctgatcaga aatcgcggac cgaaaccgac gccgccggaa agcttgtccg gttcggcgtt
     3001 gacctcgctg cgaatctgga agctggtgtc gccgatcgtc accttgtcgc cgatcttcag
     3061 atcgagccgc gccagcaagg ccggatcggc cgcggcgcca aagctgccgt cgcgctcggc
     3121 gagcacatcc tgcagcggga gcgccggatc gagcgagagc tcaccgagca tcggatagcc
     3181 gccgtcgacc gccttcatct cgacgagagc cagccggccg tcagagctgc gcgccatgcc
     3241 gcgcatggtc gcgatcaccg aaagctgtcc gcgcgagcgc agaaacgcga gttcggcggg
     3301 ttttgcttcg cgctggaaca gaacgaaggc gacgtcgccg ccaagcagat tgcgtccctc
     3361 acgcgccaat ccgtcggaca ggctggccgc gaccgatccg acacctgcga tcgccatgac
     3421 gccgagcgcg atgcaggcaa tgaatacgta gaagccacgc aagccgccgc gcagatcacg
     3481 cagggcgtag cgcaggctga gccataagcg gccgcctccc gcgggctcgg tcgcgacgcc
     3541 gagcagagct tcggacgcga cgctcatgag gccactttgc gatccgcgcc gcccggctcg
     3601 acgcgaccgg agcgcagccg caccacgcgg tcgcagcgtt gtgcgaggga agcgtcatgc
     3661 gtcaccagca ccagcgtcat gcctcgctcg gcatgcttcg tgaagagcag gtcgatgatc
     3721 tgcttgcccg tcgcctcatc gagattgcc
//
LOCUS       DATLJT010000236         1726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1620147,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000236 DATLJT010000000
VERSION     DATLJT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620147"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VM910_05395"
     CDS             complement(<1..110)
                     /locus_tag="VM910_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI62015.1"
                     /translation="MGEVTTIGLDLAKHVFQVHGVDAEGATVLRKQLRRA"
     gene            complement(186..1196)
                     /locus_tag="VM910_05400"
     CDS             complement(186..1196)
                     /locus_tag="VM910_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62016.1"
                     /translation="MTVTIGRRELLAALGGAAVVWPLAARAQQPERMRRIGVLMGVAD
                     DREGQTRVAALKQGLQELGWTNGRNIQIATRFGGADAARIQADAAELVALAPDVIVGQ
                     TTPVIRALRQATSSIPIIMAAVSDPVDQGFVSSLAHPGGNITGYVTGFSQFKYGLSGE
                     WLELLKEVAPKVTRAAVIRDPAIASGIGQFGAIQAVAPSLGVEVSPFNVRDAGEIERT
                     ITAFSHSSNGGLIVTGSALAVVHRELIIALAARNNLPAVYFERLFVARGGLVSYGPDL
                     LDQFRRAAGYVDRILKGEKPADLPVQAPTKYELVINLKTARALGLGVPPTLLARADEV
                     IE"
BASE COUNT          313 a          559 c          505 g          349 t
ORIGIN      
        1 tgcgcacgcc gaagctgctt gcgcagaacc gtcgctccct ctgcatcaac cccgtgcacc
       61 tgaaaaacat gcttcgccaa gtccaatccg atcgtagtaa cctcgcccat ggatggctcc
      121 cattcgctgc tgtttcgatc acagcagttt ggcactcaga tgccgcaagg gggccatcca
      181 ccccatcact cgatcacctc gtcggcgcgg gcgagcaggg tcggcggcac cccaaggccg
      241 agggccctcg cggtcttaag attgatcacc agctcgtact tggtcggcgc ttgcaccggt
      301 aggtcggctg gcttctcgcc cttgaggatg cggtcgacgt agccggcagc gcgccggaat
      361 tggtccaaaa gatcgggccc gtaggagaca aggccgcctc tggcgacgaa aagccgctcg
      421 aagtagaccg ctgggaggtt gtttcgggcc gctagcgcga tgattagctc gcgatggacc
      481 accgccaacg cgctccccgt cacgatcagg ccaccattcg aggagtgcga gaaggccgtg
      541 atggtacgct cgatctcgcc cgcgtcgcga acgttgaacg gactcacctc caccccgagc
      601 gacggcgcca cggcctggat ggcgccaaac tggccgatgc cggaggcgat ggccggatcg
      661 cgaatgactg ccgctctggt cactttcggt gcgacttctt tgagcagctc aagccattcc
      721 ccgctcaggc cgtacttgaa ctggctaaat ccggtaacat agcccgtgat gttcccgccc
      781 ggatgcgcca agctcgaaac gaagccctga tcgacgggat cgctgaccgc cgccattatg
      841 atcggaatgg agcttgtcgc ctgccgcagc gctcggatga ccggcgtcgt ttggccgacg
      901 attacgtcag gcgcgagcgc cactaattct gcggcgtcgg cttgtatgcg ggcggcgtct
      961 gccccaccaa agcgtgtcgc aatttggatg ttgcggccgt ttgtccatcc taattcttga
     1021 agtccctgct taagcgctgc cactcgggtc tgtccttccc gatcgtcggc aactcccatc
     1081 agcacgccga tgcgccgcat ccgctcgggc tgctgcgcgc gtgccgcgag cggccacacc
     1141 accgcagcgc cgccgagtgc cgccagcaac tcccgccgtc cgatggtgac tgtcatacgc
     1201 ccctcccgtg cgaggtgcgt aaagggaacg ataccacgcc acgagcgtgc tgtcctaaca
     1261 gcgcgtgttc ctgatgatgt cggcagcttt gtcgtagaag aaggtttgga cacagtctcg
     1321 agagcgactt tcatatagat gcgttttcgt tattggtgaa aacacgatat ttttggtagt
     1381 aagcgcgttc gactgctcat cagccgaaat cgaggattaa ttcctgatgc gcgcgcgccg
     1441 cgagcgacca cgcgataacg ccactttcgg ctgggaaatg tcactgtgat tgtcacgacg
     1501 tttccccgca ccatgctgcc gcccgccgcg tcgcggcgta agtgcgctgt ctcaggcccg
     1561 gaccggttca agatttccga gcttgccgtc tccttcgacg cggttgaggt agcgccgctt
     1621 gccgctcctg gaccgtgttc ctgatgatgt cggcagcttt gtcgtagaag aaaaaggcgt
     1681 tctttattcc cgccgcaaac ctaccttcgg gatccgcccc agacgc
//
LOCUS       DATLJT010000237         7263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_11876,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000237 DATLJT010000000
VERSION     DATLJT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11876"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1273
                     /locus_tag="VM910_05405"
     CDS             44..1273
                     /locus_tag="VM910_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62017.1"
                     /translation="MKARGARRFREQKWLLDSVVSTIGLDWDLLRMATVTAPIGFEGT
                     GDWAVVAGKAKRFDDISPSFEEVAAGREARAREAEQLGNEVTARESYLVASIYYGVAQ
                     WPIDEINDRNLALNAKKLDCYARYAARAHHKIERIDIPMGKHILPAWLHLPVAGKPPY
                     PLVIMLPGMDTFKEKLVWAYGDKMLERGFAALAIDGPGQSEPLMHGLKVTADNFADAG
                     RACIAWIDTRKDLDQNRIGVFGRSFGSYAATVLSNAIAERLCGVVVGLPCFEPGFAKI
                     FEEASPTYKNRFMFMAGYEDEAEFDKFIQGFDLRTRVSNLRCPFMVLGGELDELSPIK
                     HVFEVCKKVPGPVEIVVYQNERHAPGRLPSSQLGPHWYAVMADWLAARVRDRAPIEGR
                     HYRYVTASGKVEQRPML"
     gene            1483..2649
                     /locus_tag="VM910_05410"
     CDS             1483..2649
                     /locus_tag="VM910_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62018.1"
                     /translation="MKRYHSMIRQSAVTALAAVGLIGSAAAQSTIDIGLVQPLTGAFA
                     AAGTDVVNGAKIAADEINAKGGVLGKKLELIIEDTKSNPTEAAAVTEKLVVRDKVPVL
                     MGASASTATLAVMPKLMEYKVPMLVETSSSSKITTSGNPYIFRIAPPSEVEAAVFAKI
                     VKKIGIHKADFLVVNNDWGRGTADEFSKVLKDNGIAVGLVERMDQGAQDMNAQLVKLK
                     GSGADTLFVTTAVEQLSLVLKQAASLGLNVRLISTGGSQNPDQLITNVGKAADGTWHL
                     VFFTPWSPEATPDPTAAKAFVAEWNKRGLPFGGLTSSFRGYDGIRAITEAIRIAGKAE
                     PEAIRAALWKVNILGLNGPIKFQKDGPQGQESGQSTPNVYLVKIDSGKVTVPKI"
     gene            2928..3827
                     /gene="cyoA"
                     /locus_tag="VM910_05415"
     CDS             2928..3827
                     /gene="cyoA"
                     /locus_tag="VM910_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HVI62019.1"
                     /translation="MQRRPLLWRVSAAPIILLSLSNCSGGVLDPQGPIGGANAKILLN
                     ALGIMLVIVVPTIVAILGFGWWFRASNTRARYRPDFVYSGAIEMVVWGIPILVILFLG
                     GVIWIGAHDLDPFKPISSPAKPVEVQVVSLDWKWLFIYPEQGVASVNELVVPAGVPVH
                     FSLTSASVMNMFFVPQLGTMIATMNGMVTQVHLAADNPGEFYGQSAQYSGGGFSDMNF
                     TARAVPQDAFAQWVATARQSGSALDRSSYLALCQQSSNVRPFTYRAVDAGLFNAIVTQ
                     QLPPCPGPNIERAGATVRPRTER"
     gene            3829..5835
                     /locus_tag="VM910_05420"
     CDS             3829..5835
                     /locus_tag="VM910_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI62020.1"
                     /translation="MFGKLTWDVIPWSQPIPLVAGSVVVLIVLAILAWVVLKGYLPYL
                     WREWITSVDHKRIGVMYTLLGVVMLLRGFIDAIMMRSQQAIAFRAAGYLPPEHYDQIF
                     SAHGTIMIFFAAMPIMIGLMNFVVPLQLGVRDVAFPTLNSTSFWLTATGALLVNLSLV
                     IGEFARTGWLVYPPLSELPYSPGVGVDYYLWALLISGVGTLMTGINFVTTILKIRAPG
                     MSYLRMTIFCWTALAANLLIVAAFPVLTATLGMLALDRYLGFHFFTNDAGGNMMMFIN
                     LIWVWGHPEVYILVLPAFGIFSEVISTFSGKPLFNYRSMVAATLFICLVSYLVWMHHF
                     FTMGAGADVNAFFGISTSIIAVGTGVKIYNWLFTMYGGRIRFATPMLWSMGFLVTFTI
                     GGMTGVLLAVPPADFVLHNSLFLVAHFHNVIIAGVVFAAFAGITYWFPKAFGFKLDEG
                     WGKAAFWLSLLGFYVVFMPLYVAGLLGMTRRLQHYEVAAWRPWVLIAGVGIVIMMAAA
                     ACQVIQFVVSVRRREQLRDETGDPWDGRSLEWATASPPPPFNFAVLPNVQGAEPYWGI
                     KRRAMETQQLSAEPRYEPIEMPRNSPTGVITAFFCTMTGFALIWHIWWMVGLGLVAAY
                     AVFVWFAWRDVVEYSIPAEEVARFDRERRRAREQWLAQHAGQPA"
     gene            5832..6524
                     /locus_tag="VM910_05425"
     CDS             5832..6524
                     /locus_tag="VM910_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVI62021.1"
                     /translation="MSATDAPFERSADPYRVGRGRDWLSEGAGRGYVYATGHGEGGPA
                     SKRVIVGYGFWIFLLSDIVMFSAFFAAHAVLQTATAGGPSGRDLFNWWSIVAQTGFLL
                     VSSFTCGLSAVATEARNQLWTQVALLVTGLFGLGFLVLEGRDFAGMVMQGAGPDRSAF
                     LSSLFAVVGLHGLHVSAGLLWLGTMMAQVFVKGFRADILRRLVCFNLFWHALDIIWVA
                     LFTVVYLIGLGR"
     gene            6521..6916
                     /gene="cyoD"
                     /locus_tag="VM910_05430"
     CDS             6521..6916
                     /gene="cyoD"
                     /locus_tag="VM910_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HVI62022.1"
                     /translation="MSHETHRRDPGDVGPGFMDAAELTIREGIARYVIGFVLACVLTA
                     ASFYVLNTSLIWGPGIPVALIVLAIAQMGVHLVFFLHINTAPDNTNNVLALAFGVLIV
                     ALVIAGSLWIMDHLNQNMVPMHQMMQMQR"
     gene            complement(6964..>7263)
                     /locus_tag="VM910_05435"
     CDS             complement(6964..>7263)
                     /locus_tag="VM910_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HVI62023.1"
                     /translation="LPFLGQGGVMSIEDGYVVAACLDKYFGEPGLAFARYEEIRRERT
                     SMVVRKASENKASAFAPSLADKDRIAEEVAREWQQVRLRERMEWLYNYDATAIAI"
BASE COUNT         1297 a         2233 c         2249 g         1484 t
ORIGIN      
        1 ccgttcggct tgcacaggcg tgtaatccgc aatggagagt tcaatgaagg cgcgcggggc
       61 aagacgattt cgcgagcaga aatggctgct ggattccgtc gtctcgacca tcggcctgga
      121 ttgggatctg ctgcgcatgg ccacggtcac ggcgccgatc ggattcgagg gcacgggcga
      181 ctgggcagtg gtcgccggca aggcaaagcg cttcgacgac atctcgccga gcttcgagga
      241 ggtcgcggca ggccgcgaag cccgcgcccg tgaagctgaa cagctaggca atgaagtcac
      301 ggcacgggaa tcgtatctgg tcgcctcaat ctattatggc gtggcgcaat ggccgatcga
      361 cgaaatcaac gatcgcaatc tcgcgttgaa tgcgaagaaa ttggactgct acgcgcgata
      421 cgcagcgcgc gcgcaccaca agatcgagcg gatcgacatc ccgatgggca agcatatttt
      481 gcccgcttgg ctgcatcttc ccgtggccgg caagccgccc tacccgctcg tgatcatgct
      541 gcccggcatg gacaccttca aggaaaagct ggtctgggcc tacggcgaca agatgctcga
      601 gcgcggcttt gcggcgctgg caatcgacgg gcctgggcaa tccgaacctc tgatgcacgg
      661 gctcaaggtg acggccgaca acttcgccga tgccgggcgg gcctgtatcg cctggatcga
      721 cacgcgaaag gatctcgatc agaaccgaat cggcgttttt ggacgaagct tcggctctta
      781 cgcggcgacc gtcctctcca acgcgatcgc cgagcggctt tgcggcgttg tggtcggcct
      841 tccatgtttc gaaccgggct tcgccaagat cttcgaggaa gcgtcgccga cttacaagaa
      901 tcgcttcatg ttcatggccg gctacgagga tgaagccgag ttcgacaaat tcattcaggg
      961 cttcgacttg cgaacgcggg tatccaatct tcgctgtccg ttcatggttc tcggcggaga
     1021 gttagatgag ttgtcgccga tcaagcacgt gttcgaagtc tgcaagaagg tgcctgggcc
     1081 ggtcgagatc gtcgtctatc aaaacgagcg gcatgcaccc gggcggctcc cgtcgtcgca
     1141 actaggcccc cattggtacg cagtgatggc ggactggctg gcagcgcggg tgcgcgaccg
     1201 tgcgccgatc gaagggaggc attatcgata cgtcactgcg tccggcaaag ttgagcagcg
     1261 tccgatgttg tgaggaccga accatatgcc aacgcgttcc agctgcacca gcactcgctc
     1321 gcgcgttgac gtcgctaagc acgaacgctt gcctaacttg ccgaatgtgg cacgctcata
     1381 tttactgcta tgccgatgac gcgcacatct ccaaggcatc ggctcgatcg cggcacgtga
     1441 tctcgcactc tggccgattg agaatgaagg ggaggatgag agatgaaacg gtaccattcc
     1501 atgatccgac aaagcgcagt gaccgccctg gcggcggtcg gtttgatcgg cagcgccgcc
     1561 gcacaatcca caatcgacat cggcctggtg caaccgctta cgggtgcatt cgcggcagcc
     1621 ggtaccgacg tcgtcaacgg cgcaaaaatt gccgccgacg agatcaacgc gaaaggcggc
     1681 gtactcggta agaagctcga gctcatcatc gaagacacca agagcaatcc gacagaagcc
     1741 gcagcggtga ccgagaaact cgtcgtgcgc gacaaagtcc cggtgctcat gggtgcgtct
     1801 gcctcgacgg caacgctggc ggtgatgccg aaactgatgg aatacaaggt gccgatgctg
     1861 gtcgagacgt cgtcgtcgtc gaaaatcacg acctccggca atccgtatat ttttcgcatc
     1921 gcgccgccgt cggaggtgga agcggccgtg ttcgcaaaaa tcgtcaagaa gatcggcatc
     1981 cacaaggccg acttcctcgt ggtgaacaac gactggggcc gaggcaccgc cgatgaattc
     2041 agcaaggtgc tcaaagacaa cggtattgcc gtcggcctgg tggagcgcat ggatcagggc
     2101 gcacaggaca tgaacgccca gcttgttaag ctcaaaggtt ccggcgccga cacgctgttc
     2161 gtgaccacgg ccgtcgagca gctatcactc gtcctcaagc aggccgcctc gctcggcctc
     2221 aacgtccgct tgataagcac tggcggatcg caaaatcccg atcagctgat tacgaacgtc
     2281 ggcaaggccg ccgatggcac ctggcatttg gtcttcttta caccctggtc gccggaggcg
     2341 acgcccgacc ccacagccgc caaggccttc gttgcggaat ggaacaagag agggttgccg
     2401 ttcggcggcc tgacctcaag ttttcgcggc tatgacggca tccgcgcgat cacggaggcg
     2461 atcaggatcg ccggcaaggc tgagccggag gccattcgcg cggcgctgtg gaaggtgaat
     2521 attctcggtc tcaatggccc gatcaaattc caaaaggacg gtccgcaggg ccaggaaagc
     2581 gggcagagca cgccgaacgt ctatctggtg aagatcgaca gcggaaaggt aaccgttccg
     2641 aagatctaga tctgaatacc gctggagatg aaacatctgc gccgacgggt ccgtcgcggc
     2701 ggtattttca atgatcatac cggcggcggc cacatcgggt ttgtctacgg cgtcacaaat
     2761 caccccgagt agagatcgca gactggcgga gcggtccgtt taagaggtgg catcacaaat
     2821 tggatcacgg ccgttgagct tcaaccgagt ttcaggtaat tgcagttaag caaagcctga
     2881 caaagatgca gccaccagac tccatgtcat cgtgggcagc attccttttg caacgccgcc
     2941 ccctgctctg gcgagtgtcg gctgcgccga tcatccttct gtcgctgagc aactgttcag
     3001 gcggcgtgtt ggacccgcag gggccgatcg gcggggcgaa cgcaaaaatc ctgctcaacg
     3061 ccttgggcat catgctggtg atcgtggtgc ccacgatcgt cgcgatcctg ggtttcggct
     3121 ggtggtttcg cgcgtcgaat acgcgggcgc gctaccgacc cgacttcgtc tattccggcg
     3181 cgatcgagat ggtcgtctgg ggcattccga tcctggtgat cctgtttctc ggcggcgtca
     3241 tctggatcgg agcgcacgat ctcgatccct tcaagccgat tagctcgccg gccaaaccgg
     3301 tcgaggtgca ggtcgtctcg ctcgattgga aatggctgtt catctacccc gagcagggcg
     3361 ttgcaagcgt caacgagctt gtagttcccg ccggggtgcc ggtgcacttc tcgctgacgt
     3421 cggcctccgt catgaacatg ttcttcgtgc cgcagctcgg cacgatgatc gccacaatga
     3481 acggaatggt cacgcaggtg catctggccg ccgacaatcc cggcgagttt tatggccaat
     3541 ccgcgcaata tagcggcggt ggcttttccg acatgaactt cacggcgcgc gcggtgcccc
     3601 aagacgcttt cgcgcaatgg gtcgccacgg cgcgccagag cggatctgca ttggaccgat
     3661 cgagttatct cgcgctgtgc caacaaagca gcaacgtacg tcctttcacc tatcgcgcgg
     3721 ttgatgcggg cttgttcaat gcgatcgtga cccagcagct tccgccgtgt ccggggccaa
     3781 acatcgaacg cgcgggcgcg acggttcgtc cgcgaacgga gcgctgacat gttcggcaaa
     3841 cttacctggg atgtaatccc atggagccag ccgatcccgc tcgttgccgg ctcggtcgtg
     3901 gtgttgatcg ttctcgcgat cctcgcgtgg gtcgtgctca aaggctattt gccttacctc
     3961 tggcgcgagt ggatcacgag cgtcgaccac aagcgcatcg gggtgatgta caccctgctc
     4021 ggcgtggtca tgctgctgcg ggggttcatc gacgcgatca tgatgcgctc gcagcaagcg
     4081 atcgcgtttc gcgccgcggg ttatcttccg ccggagcact acgaccagat cttttccgcc
     4141 cacggcacga tcatgatttt cttcgctgcc atgccgatca tgatcgggct catgaacttc
     4201 gtcgttccgc tgcagctcgg cgtccgcgac gtcgccttcc cgacgctgaa ttcgacgagc
     4261 ttctggctca ccgcgaccgg cgcgctgctg gtcaacctct cgctggtcat cggcgagttc
     4321 gcgcgcacgg gctggctggt ttatccgccc ttgtccgagc ttccctactc tcccggcgtc
     4381 ggcgtcgatt actacctatg ggcgctgctg atctcggggg tcggcacgct gatgaccggc
     4441 atcaacttcg ttacgacgat tctcaagatc cgcgcgcccg gcatgagcta cttgcgcatg
     4501 accattttct gttggaccgc gctcgccgcc aacctgctga tcgtcgccgc ctttccggtc
     4561 ctcaccgcca ccttgggaat gctggcgctc gaccgctacc tcggcttcca tttcttcacc
     4621 aacgacgccg ggggcaacat gatgatgttc atcaacctga tctgggtctg gggccatccc
     4681 gaggtctaca tcctggtcct gccggcgttc ggcatcttct ccgaggtgat ctccacgttt
     4741 tccggcaagc ctttgttcaa ctatcgctcc atggtcgcgg cgacgctgtt catctgcctc
     4801 gtctcctacc tcgtctggat gcaccatttc ttcaccatgg gggcgggcgc cgacgtcaac
     4861 gccttcttcg gcatctccac cagcatcatc gcggtcggca ccggggtgaa gatctacaac
     4921 tggctgttca cgatgtacgg aggccgcatc cgtttcgcca ccccgatgct ctggtcgatg
     4981 ggctttctcg tgacgttcac gattggcggc atgaccggcg tgcttcttgc cgtcccgccc
     5041 gccgactttg tgctgcacaa cagcctgttt ctggtcgccc atttccataa cgtcatcatc
     5101 gctggtgtgg tgttcgccgc ctttgccggc atcacctact ggttccccaa ggcgttcgga
     5161 ttcaaacttg acgagggctg gggcaaggcc gcgttctggc tctcgctgct tggcttttac
     5221 gtggtgttca tgccgctcta tgtcgccggc ctgctcggca tgacgcggcg cttgcagcac
     5281 tacgaagtgg cggcgtggcg tccatgggtg ctcatcgcgg gagtcggcat tgtgatcatg
     5341 atggcggccg cggcctgcca ggtgattcag ttcgtcgtga gcgtccggcg gcgcgagcaa
     5401 ttgcgcgatg agacgggcga tccctgggac ggccgctcgc tcgaatgggc gaccgcatca
     5461 ccgccgcccc ccttcaattt cgcggtgctg ccgaacgtgc agggcgcgga gccctattgg
     5521 gggatcaagc ggcgcgcgat ggagacgcag caattgtcgg cagagcccag gtatgagccg
     5581 atcgagatgc cgaggaacag cccgaccgga gtcatcaccg ccttcttctg cacgatgacc
     5641 ggcttcgcgc tgatttggca catctggtgg atggtgggcc tggggctcgt cgccgcctat
     5701 gcggtattcg tgtggttcgc gtggcgcgac gtcgtcgagt attcgatacc ggcggaggag
     5761 gtcgcccggt tcgaccgcga gcgccggcgc gcccgcgagc aatggctcgc gcaacacgcg
     5821 ggccagccgg catgagcgcg accgacgccc cgttcgagcg ctcggccgac ccataccggg
     5881 tgggccgcgg ccgtgattgg cttagcgagg gcgccggccg gggatacgtc tatgcgacgg
     5941 gtcacggaga aggcggcccg gcctccaagc gcgtgatcgt cggctacggc ttttggatct
     6001 ttctgctcag cgacatcgtg atgttctccg ccttcttcgc cgcgcatgcc gtgctgcaaa
     6061 cggccaccgc gggcggacct tccgggcgcg acctgttcaa ctggtggagc atcgtggcgc
     6121 agacggggtt tttgctcgtc tcgagcttca cctgcgggct atcggccgta gcgaccgagg
     6181 cacgcaatca gctgtggacg caggtcgcgc tgctggtgac ggggttgttc gggctgggtt
     6241 tcctggtgct cgaggggcgg gattttgccg gcatggtgat gcaaggcgcc ggccccgacc
     6301 gcagcgcttt cctctcgtcc ttgttcgccg tcgtcggcct gcacggactg catgtcagcg
     6361 cggggctcct ctggctgggc acgatgatgg cgcaggtctt cgtcaaaggc tttcgcgcgg
     6421 atattctgcg ccgcctggtg tgcttcaatc tgttctggca tgcgctcgac atcatctggg
     6481 ttgcgctgtt cacggtggtc tacctgatag gattgggccg atgagtcacg agacccacag
     6541 acgagacccg ggcgacgtgg ggcccggatt catggatgcg gcggaactga cgattcgcga
     6601 agggatcgcg cgctacgtca ttggatttgt gctcgcgtgc gtgctgactg cggcgtcgtt
     6661 ctacgtgctc aacacctccc tgatctgggg tccgggcatc cctgttgcgc tgattgtgct
     6721 cgcaatcgcg cagatgggcg tgcatctggt tttcttcctg cacatcaaca cggctcccga
     6781 caacaccaac aacgtgctgg cgctggcgtt cggcgtcctg atcgtcgcgc tggtgatcgc
     6841 cggctcgctc tggatcatgg atcacctcaa ccaaaacatg gtgccgatgc atcagatgat
     6901 gcagatgcaa cggtgatcgt tcggcaacac gccaaggtaa gatgcatgcg cgagccgccg
     6961 cgatcaaatc gcgatcgcgg tcgcgtcata gttatatagc cactccatcc gctcccgcag
     7021 gcggacctgc tgccactcgc gcgcgacctc ctcggcaatc cggtccttgt cggcaaggct
     7081 cggcgcaaac gcgctcgcct tattctcgct ggccttgcgg acaaccatcg aggtccgctc
     7141 cctgcggatc tcctcgtagc gggcgaaagc caagcccggc tcgccgaaat acttgtcgag
     7201 acaggcggcg acgacataac cgtcctcgat cgacatcacg ccgccctggc ccaggaacgg
     7261 cag
//
LOCUS       DATLJT010000238         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2769311,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000238 DATLJT010000000
VERSION     DATLJT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2769311"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VM910_05440"
     CDS             complement(<1..384)
                     /locus_tag="VM910_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI62024.1"
                     /translation="MPSDEMIRSLGRPLSPGAIALMLMLCFSWGFNQVAVKLALPDIP
                     PMLQATIRSAAALPVLLIIGWLRGVRFFRNDGTLRAGLFAGLLFGIEFVLIYQGLRFT
                     SASRAAVFLYTAPFFVALGSYQVLGE"
     gene            complement(422..913)
                     /locus_tag="VM910_05445"
     CDS             complement(422..913)
                     /locus_tag="VM910_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HVI62025.1"
                     /translation="MISSKPAFPAPDHDHGRCTAEGIAHAERVCRQKAQKFTPIRRQV
                     LQALLASHRPLGAYEVIDALATQMPRPAPITVYRALDFLMANGLVHRIESRNAYLACA
                     HDHDAAALVAFLICDRCGAVGEIPSAPVAQSLKSAARASGFAPKLSVVEITGTCAHCQ
                     KAA"
     gene            1133..1612
                     /locus_tag="VM910_05450"
     CDS             1133..1612
                     /locus_tag="VM910_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI62026.1"
                     /translation="MRSSVDINFLFTLGEVQRLVRAYADKAAARYGITRAQWAVLAKV
                     ERYEGMKQTELAEQMEMQPITLTRLIDKLCDNGWIERRGDETDRRVNRLYLKKAARPL
                     LGKLAGLRSELTATALDGINPADAHRMLAQLETIKENVRTAIQTGESEVPRKEQRYG"
     gene            1605..>2228
                     /locus_tag="VM910_05455"
     CDS             1605..>2228
                     /locus_tag="VM910_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVI62027.1"
                     /translation="MADPILKFPPEQKGVPPAKPRIKLAAEPRRRLMAGLRRYRRFLL
                     LVVLPLATAIGGLVFYLNGGRYVGTDDAYVGAQKVLITPDISGKIDKVVVKEGQHVNK
                     DDVLFEIDPVPFKLAVDQARAQLEQAKTTYDNLVANVKIYGQMLDLAQQAVDLKQRDV
                     ERKQALVAQKVGSQLDLDNSANAMVTAGAQAQFVRQQLSNAKAQLLGN"
BASE COUNT          472 a          689 c          704 g          363 t
ORIGIN      
        1 ttcgcccagc acctgatagg agccgagcgc gacaaagaac ggcgcggtgt agaggaacac
       61 cgcggcgcgg gaggccgagg tgaagcgcag gccctgatag atcagaacga actcgatgcc
      121 gaacaagagt ccggcgaaca gacctgcacg caacgtgccg tcgttccgga aaaatctcac
      181 gccgcgcagc cagccgatga tcagcagcac cggcagcgcc gcggcggaac ggatcgtcgc
      241 ttgcagcatc ggcggaatgt caggcagcgc gagcttcacc gcgacctggt tgaagcccca
      301 ggagaagcag agcatcagca tcaacgcgat ggcacccggg ctgagcggac gcccgagcga
      361 gcggatcatt tcatctgaag gcatatgtcc tcgtcaccgg gcttgacgcc cgtgttgttg
      421 ttcaggcggc tttctggcag tgcgcgcagg taccggtgat ttcgacgacg gagagcttgg
      481 gcgcaaagcc cgaggcacgc gccgctgatt tcaggctctg cgccacgggc gccgaaggaa
      541 tttcgccgac cgcgccgcag cgatcgcaga tcaggaacgc gaccagcgcg gccgcatcat
      601 ggtcatgcgc gcaggcgaga taggcgttgc ggctctcgat gcggtgcacg agaccgttcg
      661 ccatcaggaa atcgagggcg cgatagaccg tgatcggagc cggccgcggc atctgcgtgg
      721 caagtgcgtc gatcacctca taggccccga gcggccgatg gctcgccagc agcgcctgca
      781 gcacctgccg gcggatcggt gtgaatttct gtgccttctg ccggcatacc cgctcggcat
      841 gcgcgatccc ttccgcagtg cagcggccat gatcgtgatc gggcgcggga aaggctggtt
      901 tcgacgaaat cattgcaacc aatatgtcat gcgacccacg cgggccatct gagaccacta
      961 taacacaagg aatttgccct ccggcatggt ctccgggcgg agtgccgagc catgttgttt
     1021 cggggggtca ggcctccatt ttcctcgaca tgaaaaaatc ataagctagc ttattatatt
     1081 caaagcttat agagtagaaa acctccagac ttcagattac agggcgacgg ccatgcgcag
     1141 ttcagtggac atcaatttcc tttttacgct cggcgaagtg cagcgcctgg tgcgcgccta
     1201 tgccgacaag gcggccgccc gctatggcat cacgcgcgca caatgggcgg tgcttgccaa
     1261 ggtcgagcgc tacgagggca tgaagcagac ggaactcgcc gagcagatgg agatgcagcc
     1321 gatcacgttg acgcggctga tcgacaagct ctgcgacaat ggctggatcg agcgccgcgg
     1381 cgacgagacc gaccgccgcg tcaatcggct ctatctgaag aaggccgcgc gtccgctgct
     1441 cggcaaactg gccgggctgc gctcggaact gaccgcgacc gcgctcgacg gcatcaatcc
     1501 ggccgacgct caccgcatgc tcgcgcaatt ggaaaccatc aaggaaaacg tgcgcacggc
     1561 cattcagacc ggcgagagcg aagtgcccag gaaggagcag cgttatggct gatccaatcc
     1621 tgaaattccc gcccgagcag aagggcgtcc cgccggcgaa gccgcgcatc aagctcgccg
     1681 cagaaccgcg gcgccgcctg atggcgggac tgcggcgcta tcgccgcttt cttctcctgg
     1741 tcgtgctgcc gcttgcgacc gcgatcggcg ggctcgtgtt ctatctcaat ggcggccgct
     1801 acgtgggcac cgacgatgcc tatgtcggcg cgcagaaggt tctgatcacc cccgacatct
     1861 ccggaaaaat cgacaaggtc gtggtgaagg aaggccagca cgtcaacaag gacgacgtac
     1921 tgttcgagat cgatcccgtt cccttcaagc ttgcggtcga ccaggccagg gcgcaactcg
     1981 aacaggccaa aacgacctac gacaatctcg tcgccaatgt gaaaatctac ggccagatgc
     2041 tcgatctggc gcagcaggcg gtcgacctga agcaacgcga cgtcgagcgc aagcaggcgc
     2101 tggtcgccca gaaggtcggc tcgcagctcg atctcgacaa ttcggcgaat gccatggtga
     2161 ccgcgggcgc gcaggcgcaa ttcgtgcggc agcagctttc caatgcgaag gcgcaactgc
     2221 tcggcaat
//
LOCUS       DATLJT010000239         4415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1161032,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000239 DATLJT010000000
VERSION     DATLJT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161032"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..772)
                     /locus_tag="VM910_05460"
     CDS             complement(5..772)
                     /locus_tag="VM910_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI62028.1"
                     /translation="MNIISGFADISLLQLLLVAGVALFAAIIGGLAGYGTGALMPLVL
                     VPLIGAEPVVPIIAISAIFTNLSRSAAYFRYADRRRSAIVIAAAAFTTALGAYGYTRL
                     TNAGAALVIGTMLIASVPLRRLLHRRAVKIGDRTLAAGAVGYGVLVGGTAGSGVILLS
                     LLMAAGLEGASVIATDAVISVVTSLIKISVFGLAGVLTAQVIAFAVLIGAVALPGAFL
                     AKAFVERMPVHVHTAILDAAVIVGGTVMMSAALRQLV"
     gene            complement(783..2039)
                     /locus_tag="VM910_05465"
     CDS             complement(783..2039)
                     /locus_tag="VM910_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HVI62029.1"
                     /translation="MTAARVLRDAPWLRPGAAVRVLKLLNGDGEEARVVGGAVRNALL
                     GLPTGDIDIATTALPEEVIRRAKAAGIKSVPTGIEHGTVTLVVDTQPFEVTTLREDTE
                     TFGRKAKVAFGRDWPGDAHRRDFTINALSVGIDGVVHDYVGGLADIAARRVRFIGDPG
                     QRIEEDYLRILRFFRIHAAFGAGEVDRAGYLACIRGRAGLANLSAERVRMEMLKLLTA
                     DGAVAAIEAMNDGGLLQAIFGSVAYGGPFAAMIAAEGALSLQADAIRRLAALGVAITE
                     DARRLATRLRLSNAEAKRLDAMGHRWWRLAGMDDARARRLLYRLGEADFRDRVMLAWA
                     RAAGDGNAPRWVELATLPQRWSAPKFPLKAADFIARGVAEGPLLGHVLTLAEDAWLAA
                     DFPLDRPALKALADQAVARHNRDAKS"
     gene            complement(2036..2299)
                     /locus_tag="VM910_05470"
     CDS             complement(2036..2299)
                     /locus_tag="VM910_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HVI62030.1"
                     /translation="MIRPVLTEIGIFLIPFGIYALFLFATRSGVLVQSSWPIQLVARL
                     VLGSLLLVIASLVMLAHFSGGSPDSTYVPAHIENGRLVPGVEK"
     gene            complement(2296..2964)
                     /locus_tag="VM910_05475"
     CDS             complement(2296..2964)
                     /locus_tag="VM910_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVI62031.1"
                     /translation="MNEPKLKIAPEMSAAEFFARARERLSFDVPPGLLDPGIIPESGD
                     SGTERMLEIIAREQPIRPAAVLVPVVDHPQPTVLLTQRSAHLADHAGQIAFPGGKIDA
                     TDASPLDTALREAREEVGLKREYIEPIGYLGLYGTSFGFRILPTLARVKPGFKLVLSQ
                     AEVDDTFEVPLSFLMNPANHQVQSKEFRGMLRSYYAMPYGPRYIWGATAGMLRMLYER
                     IYLS"
     gene            complement(2961..3569)
                     /locus_tag="VM910_05480"
     CDS             complement(2961..3569)
                     /locus_tag="VM910_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HVI62032.1"
                     /translation="MAKQGQTTTNLDSLTIAAREATNKTPAGKGLPPVHLWNPPFCGD
                     LDMRIASDGTWFYMGTPIGRHALVRLFSTILKREGNKHFLVTPVEKVGIRVDDAPFLA
                     VEMLKDNDSQGVLLRFRTNMDDWVNCDAAHRLRFERAADGGLTPYLHVRADLWAKVTR
                     ALYYDLVDLGEERFVDGQEMFGVVSAGEFFPMADADEVRAAL"
     gene            3819..>4415
                     /locus_tag="VM910_05485"
     CDS             3819..>4415
                     /locus_tag="VM910_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI62033.1"
                     /translation="MAESAEKLEDAIVRSAEQVSGQVRAAKDAVSTVIFGQDRVIENT
                     LVTIMSGGHALLIGVPGLAKTKLVETLGVTLGLDAKRIQFTPDLMPSDILGAEVLDES
                     STGKRSFRFISGPVFAQLLMADEINRASPRTQSALLQAMQEQHITVAGARHDLPKPFH
                     VLATQNPLEQEGTYPLPEAQLDRFLMEIDVDYPDRDAER"
BASE COUNT          859 a         1447 c         1398 g          711 t
ORIGIN      
        1 gtcgtcagac aagctgccgc agcgccgccg acatcatcac cgtcccgccc acgatcaccg
       61 cggcgtccag gatcgcggtg tgaacatgca ccggcattcg ctcgacaaag gcttttgcga
      121 ggaacgcgcc gggtagcgcg accgcgccga tcaacacggc aaaggcgatc acctgcgcgg
      181 tgaggacgcc ggccagcccg aacacggaaa tcttgatcag actggtgacg accgagatca
      241 cggcatcggt tgcgatcacg gacgcgcctt cgagccccgc cgccatcaac agcgacaaca
      301 ggatcacgcc tgagcccgca gtgccgccga ccaggacgcc gtagccgacg gcgccggcgg
      361 caagcgtgcg gtcgccgatc ttcaccgcac ggcggtgcag cagacggcga agcggcacgc
      421 ttgcgatcag catggtgccg atcaccaggg ccgcccccgc attggtcagc cgcgtatagc
      481 cgtaggcgcc gagcgcggtg gtaaaagcag cagccgcgat cacgatcgcc gagcggcggc
      541 ggtccgcata gcggaagtac gcagcgctgc gggacagatt ggtgaagatc gccgagatcg
      601 cgatgatcgg caccaccggt tcggcaccga tcagcggcac cagcaccagc ggcatcagag
      661 ctccggtgcc gtagcccgcg agcccgccga tgattgcggc aaacagcgcg acgccggcaa
      721 ccaggaggag ttgcaacagc gagatgtcgg caaaaccgga aatgatattc acggagtgac
      781 gttcaagatt tggcgtcgcg gttatggcgc gccaccgcct ggtcggcaag cgccttgagc
      841 gcaggccgat cgagcgggaa gtcggcggcg agccaggcgt cttcggcaag cgtcagcacg
      901 tgtccgagca acggtccctc ggcgacgccg cgcgcgatga agtcggcagc tttcaacgga
      961 aatttcggcg cgctccagcg ctgtggcagc gtggcaagct caacccagcg cggcgcattc
     1021 ccatcgcctg cggcgcgtgc ccaggccagc atcaccctgt cgcgaaaatc cgcttcgccc
     1081 aggcgataga gcagccgccg ggcgcgcgcg tcgtccatgc ccgccagccg ccaccagcgg
     1141 tggcccatgg catcaagccg cttcgcctcg gcgttggaca accgcaggcg cgtcgcaaga
     1201 cgccttgcat cctcggtgat ggcgacgccg agcgcggcga ggcgccggat tgcgtccgcc
     1261 tgtagcgaaa gcgcgccctc ggccgcaatc atcgccgcaa agggcccgcc atacgcgacg
     1321 ctcccaaaga tcgcctgcag caatccgccg tcgttcatcg cctcaatcgc cgcgaccgca
     1381 ccatcggctg tgaggagctt cagcatttcc atccgcaccc gctcggccga cagattggca
     1441 agacccgccc ggccgcggat gcacgcgaga tagccggcac gatccacctc gcccgcgccg
     1501 aacgccgcat ggatgcggaa gaagcgcagg atgcgcaaat aatcctcttc gatcctttgt
     1561 ccggggtcgc cgatgaagcg caccctcctt gccgcaatat cggcgagacc tccgacatag
     1621 tcgtgcacca cgccgtcgat gccgaccgac agcgcattga tggtgaagtc gcgccgatgc
     1681 gcatcgcccg gccagtcccg gccgaacgca accttcgcct tgcggccgaa ggtctcggtg
     1741 tcctcgcgga gcgtcgtgac ctcgaacggc tgcgtatcga ccacgagcgt gaccgtgccg
     1801 tgctcgatac ccgtgggcac gctcttgatg cccgccgcct tcgcgcggcg gatcacctcc
     1861 tccggcaagg cggtggtcgc gatgtcgatg tcgccggtcg gaaggccgag cagcgcgttg
     1921 cggaccgcgc cgccgaccac acgtgcctct tcgccgtcgc cgttcagaag tttcagcacg
     1981 cgcacggcag caccaggcct gagccagggc gcatcgcgga gaactcgtgc agcggtcatt
     2041 tctcaactcc gggcacgagc ctgccgttct cgatatgagc ggggacgtag gttgagtcgg
     2101 gcgaaccgcc cgagaagtgc gcaagcatga cgaggcttgc gatcacgagc aacagggatc
     2161 cgagcaccag ccttgcgacc aactggatcg gccaggagga ctgcaccagg acgccggagc
     2221 gggttgcgaa gagaaacagc gcgtagatgc cgaacgggat caggaaaatg ccgatctcgg
     2281 tcagcaccgg acggatcatg acagatagat ccgctcatac agcatccgca gcattccggc
     2341 ggtcgcgccc cagatgtagc gcggcccgta aggcatggcg taataggaac gcagcatgcc
     2401 gcgaaattcc ttgctttgga cctgatggtt cgcagggttc atcaggaacg acaagggcac
     2461 ctcgaacgtg tcgtcgacct cggcctggct aaggacgagc ttgaagccgg gcttgacccg
     2521 cgcgagcgtc ggcaggatgc gaaatccgaa gctcgtcccg taaagaccga gatagccgat
     2581 cggctcgata tactcgcgct tcaggccgac ctcctcccgc gcttcgcgca atgcggtgtc
     2641 gagcggagag gcatcggtcg catcgatctt gccaccggga aaggcgatct ggccggcgtg
     2701 gtcggcaaga tgcgccgagc gttgcgtcag cagcaccgtc ggttgcggat ggtcgaccac
     2761 gggaacgagg acggctgcag ggcggatcgg ttgttcgcgc gcgatgatct ccagcatccg
     2821 ctcggtgccg gaatcgccgc tttcggggat gatgcccgga tcgagcaggc ctggcggcac
     2881 gtcgaaggaa agccgctcgc gggcgcgagc gaaaaattcc gcggcactca tttcgggcgc
     2941 gatcttcagc ttcggctcgt tcaaagcgcg gccctcacct cgtccgcatc ggccatcggg
     3001 aaaaactcgc ccgcggacac cacaccgaac atctcctgac catccacgaa tcgctcctcg
     3061 cccaaatcga cgagatcgta atacagcgcg cgcgtgactt tagcccagag atcggcgcga
     3121 acgtgaagat agggcgtcag cccgccgtcg gcagccctct caaagcgcag gcggtgcgcg
     3181 gcgtcgcaat tgacccaatc gtccatgttg gtgcggaagc gcaggagcac gccctgcgaa
     3241 tcgttgtctt tcagcatttc gacggcaagg aagggcgcgt catcgacgcg gatgccgacc
     3301 ttctccaccg gcgtcaccag aaagtgcttg ttcccctctc gcttcagaat ggtcgaaaac
     3361 agccgcacca gtgcgtgccg tccgattggc gtgcccatgt agaaccaagt accatccgag
     3421 gcgattcgca tgtcgagatc gccgcaaaac ggcggattcc acaaatgcac cgggggcagc
     3481 ccctttccgg caggcgtttt gtttgtcgcc tcccgtgcag cgatggtcag gctatccaga
     3541 ttggtagtgg tctgcccttg cttcgccatg gtttgcctta acgtctgatc gttcgaattt
     3601 ggcacgtttg gtgcaccggt tcgttgatgt ggagttccac cagaaaaata ccgggtcgag
     3661 gtcgccacat cgtgatgccg ttaatacccc gaaatgccgg taatgtgggg atagtttaat
     3721 tcaatgaata catggccttt gcacgagttt agcatgggtc catggcacat cgtcgccctc
     3781 cggcggcggc ttggttcgag gacttgaagg agcgacggat ggcggaaagt gcggaaaagc
     3841 tcgaggatgc gatcgttcgc tccgcggaac aggtctcggg ccaggtccgc gcggcaaagg
     3901 acgccgtgtc caccgtcatt ttcggccagg acagggtgat cgaaaacacc ctggtgacga
     3961 tcatgtccgg cggccacgcg cttttgatcg gggtgcccgg cctcgccaag accaagctgg
     4021 tcgaaaccct gggcgtcacg ctcggactcg acgccaagcg catccagttc acgcccgatc
     4081 tgatgccgtc ggacattctg ggcgccgagg tgctggacga aagttcgacc ggcaagcgct
     4141 ccttccgctt catctcgggc ccggtgtttg cgcagctctt gatggccgac gagatcaacc
     4201 gtgcgagccc ccgcacccaa tcggcgctgc tgcaggccat gcaggagcag cacatcacgg
     4261 tcgccggcgc gcgccatgat ctgccaaagc cgttccatgt gctcgcaacg cagaacccgc
     4321 tggagcagga aggcacctat ccgctgccgg aagcgcagct cgaccgcttc ctgatggaaa
     4381 tcgacgtcga ctatcccgat cgcgacgccg agcgg
//
LOCUS       DATLJT010000240         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4147865,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000240 DATLJT010000000
VERSION     DATLJT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147865"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM910_05490"
     CDS             <1..274
                     /locus_tag="VM910_05490"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62034.1"
                     /translation="VARKDLPASDLKGLVAWLKANPDKATEGTAGPGSGQHVSGIYFQ
                     DVTKTRFQFVPYRAAPPRSCATSWPDTSISHSIKPLRLCRMCATAE"
     gene            190..600
                     /locus_tag="VM910_05495"
     CDS             190..600
                     /locus_tag="VM910_05495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62035.1"
                     /translation="MRDLVAGHIDLTFDQAITALPHVRNGGVKAYAVTSNKRLAAAPD
                     IPTVDEAGAPGVYISTWYGLWVPKGTPPEAIRKLTIAAMDALADPTVRQRLADLGQEI
                     PPPEQQTAEALAAHQKAEIEKWWPLIKAANIKAE"
     gene            608..1837
                     /locus_tag="VM910_05500"
     CDS             608..1837
                     /locus_tag="VM910_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI62036.1"
                     /translation="MDLIVRNARLADRSSDELIDIGVEQGRIVALEHALAGEAEVYDA
                     KGCLACPGLIETHIHLDKSRIIDRCTPRKTLSPVKGVSPLKESMSVEDVHTRAERTLQ
                     DCIKHGTTRIRTQVEVDPAIGMRGFEGVQSLIADYKWAIDIEICVFPQEGLTNYPGTD
                     ELLVEGLKRGATVVGGAPRYDTDQAGQIRRIFELAREFDVDIDIHLDVGPTADAMNIY
                     LVRELTEQFKRGGRVVVGHMAKLSLLPPEQVAALARSLANTGIGVTVLPTTDLFLMGR
                     DRDHAVVRGVADANLLCEHGVNCSLSSNNILNPATPYGDCSLIRMANLYANVLQVDRP
                     ERLRACFGMLTDRSARLLNLTDYGFAVGRPADIVIVNAQSAEQAIAEIAQPLAVFKHG
                     RQTVVWHPPQLLRPRQY"
     gene            complement(1853..>2234)
                     /locus_tag="VM910_05505"
     CDS             complement(1853..>2234)
                     /locus_tag="VM910_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62037.1"
                     /translation="DGTQNLQPLGEGAKVVQLTLAGKDSRPATQVTLFDQGLVQMVQA
                     AVTGLPPKQPFVLALSDDPKGGGRLEPLAGFVTNPAGAAIVNTIGPIRQVTQSSGATP
                     KRYLVIAPGTPAQLGEPVQVQTQQ"
BASE COUNT          428 a          710 c          683 g          413 t
ORIGIN      
        1 cgtggcaaga aaggatctgc cggccagcga tctcaagggc ctggtcgcat ggcttaaggc
       61 caatcccgac aaggcgaccg aaggcacggc cggacccggt tccgggcagc acgtcagcgg
      121 gatttacttt caggacgtca cgaagacccg cttccagttc gtgccgtatc gtgcggctcc
      181 gccgagatca tgcgcgacct cgtggccgga cacatcgatc tcacattcga tcaagccatt
      241 acggctctgc cgcatgtgcg caacggcgga gtgaaagcct acgccgtgac ctcgaacaag
      301 cggttggccg cggcgcccga catccccacg gtcgacgagg ccggtgcgcc cggtgtgtat
      361 atctcgactt ggtatgggtt gtgggtgccg aaaggcactc cgcctgaagc gatccgcaag
      421 ctcacgatcg ccgcaatgga cgcgcttgcc gacccaacgg tgcgccagcg gctagctgac
      481 ctcggtcaag aaattccgcc accggagcag caaaccgcag aagcgctggc cgcgcatcag
      541 aaggccgaga tcgagaaatg gtggccgctg atcaaagcgg ccaacatcaa ggccgagtga
      601 atctcacgtg gatctcatcg tccgcaacgc acgtcttgcc gatcgatcat cggacgaact
      661 catcgatatt ggggtggaac aaggtcgcat cgtcgccctt gagcacgcgc tcgctggcga
      721 ggccgaggtt tatgacgcca aggggtgcct cgcctgtcct gggctgatcg agacccacat
      781 tcatctcgac aaatcgcgga tcatcgaccg ttgcacgcca cgcaaaacac tcagtccggt
      841 gaaaggcgtt tcaccactga aggagagcat gtcggtcgag gacgtgcaca cgcgcgccga
      901 gcgcaccctg caagactgca tcaagcacgg caccacccgc atacgcacgc aggtcgaggt
      961 cgatcccgcc attggcatgc gcggcttcga aggggtgcag tcgctgattg cagattacaa
     1021 gtgggcaatc gacatcgaga tctgcgtctt tccgcaggaa gggctcacca attatcccgg
     1081 aaccgatgag ctgctggtag aaggcttgaa acgcggcgcc accgtggtcg gcggcgctcc
     1141 gcgttacgat accgatcaag caggtcagat caggcgcatt ttcgagctcg ctcgcgagtt
     1201 cgacgtcgac atcgacattc atctcgacgt tggcccgacc gccgatgcca tgaacatcta
     1261 tctggtgcgc gagcttacgg agcaattcaa gcgtggcgga cgggtcgtgg tcggccatat
     1321 ggccaagctg tcgctgttgc cgccggagca ggtggcagcg ttggcacgca gcctggcgaa
     1381 caccggcatc ggcgtcaccg tcctgcccac caccgatctc tttctcatgg gccgggaccg
     1441 cgaccacgcc gtcgttcgcg gcgtagcgga tgcaaatctg ctctgcgagc acggggtgaa
     1501 ttgttcgctc tcctcgaaca acatcctcaa ccctgccacc ccctatggcg actgttcgct
     1561 cattcgcatg gccaatctct acgccaacgt tctacaggtc gatcgtcccg agcggctgcg
     1621 cgcgtgtttc ggcatgctca cggatcggtc ggcgcggctg ctcaatctga ccgactatgg
     1681 ctttgccgtc ggccgcccgg ctgacattgt catcgtcaat gcgcaaagcg ctgaacaggc
     1741 gatcgccgaa atcgcccagc cgctcgcggt attcaagcat ggaaggcaga cggtcgtgtg
     1801 gcacccgccg caactgctga gaccgcggca gtactgaaat cgtggcgacg cctcattgct
     1861 gcgtttgtac ttgcaccggc tcgcccagct gcgccggtgt gccgggagcg ataacaagat
     1921 agcgcttcgg cgtcgcgccg ctgctttgcg tgacctgtcg gatcgggccg atggtgttga
     1981 cgatggcggc gccggcagga ttggtcacaa atccggcaag cggctctaat cgtccgccgc
     2041 cttttggatc gtcggagagc gccagcacga atggttgctt gggcggcagt ccggtcaccg
     2101 cggcctggac catttgcacg aggccctggt cgaatagcgt cacctgcgtt gccggcctgg
     2161 agtccttacc ggccaacgtg agctgcacga ctttcgcccc ttcaccgagc ggctgcaggt
     2221 tctgggtccc atct
//
LOCUS       DATLJT010000241         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3228819,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000241 DATLJT010000000
VERSION     DATLJT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3228819"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..365)
                     /locus_tag="VM910_05510"
                     /pseudo
     CDS             complement(210..365)
                     /locus_tag="VM910_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            complement(379..1955)
                     /locus_tag="VM910_05515"
                     /pseudo
     CDS             complement(379..1955)
                     /locus_tag="VM910_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
BASE COUNT          520 a          785 c          734 g          491 t
ORIGIN      
        1 tggcgtgctg acgtcgatcc ggctggcgac ggccgctgcg tccgcgccat actttcccgt
       61 cttctcgatc ctgttcgaaa tatcctgcac cgtcgtgctg atcgctatga tccgtgacct
      121 tgcgcagcga acgatgttca cggcggcgtt gcagaaaatg tgaggcgacg gggcagcgtc
      181 ggccgtccgc cgcgtgcccc actcaccctt cacggtttca cgcgtgctcc cgcgagacgt
      241 cgtggtcggt gtagtccggc taatatcgtt tcaacgccca tcccctgcgc gatcaggtcg
      301 aagccgccgc gcccggcata gggtccgtca taaccgagtc cggagagctg cgcgtagatc
      361 agcctcaggt gttgcggctc aggccgccgc cggctcgggt agcgcgggct cgtcagccgc
      421 gtccagctcg gcgatctgcc cctcgacctg accgatcaaa tagtgcgtca gcggcgcgag
      481 gtagagatag aacaccgtca ttacgatgac cgtgcgtatc gcccgcgggt tcttgatcag
      541 acactcgatc aggatgcgcc agacatgacg gcgcatctca ggccggtgcc aagtcacatg
      601 gtgcataacg cggaagaacc gatcgatctc gcgccaggag tcgcggacta acacacgcgc
      661 gccgagatcg acccgatcga gcgacaacag caaccggcgc acccgcccga aaaacgccga
      721 cgggtcatag acccgcaaca ccactccgac atagtcggca aggatgtcgc ggcgtggacg
      781 caacgtgtcg aagttgagcc cagcgagaca aagatcgccg gcgcggcccg acgaatcgga
      841 atcgtggccg gcgtgcagcc gtccctcacg ttcgagtcgc cgggtcaact gcgtgttcgg
      901 cagcgcatac aagaggctga acatattcac cgggatcgtc gcgtcttcga tacagcgcgt
      961 catcgctttg attacgctcg ccttctcgct gtcgaagcca acgatgaacc ccgcaacgac
     1021 aaaaattccg gcatcgtata tgcggcgtac gctctcgggg atgctgcggc gcgtgttctg
     1081 cttcttttgc gtcgtaacca gcgcttcctc gtcgggcgtc tcgatcccga tgaacacggc
     1141 gatgaaattc gccttgcgca ttagccgcag cagctcgagg tcatcggcaa gattgagcga
     1201 tgcctcggtc gtcagcataa acgggaattt gtgctgcttc tgccactcga tcagatgcgg
     1261 caggaattcc ttcactgcct tcttgttgcc gatcagattg tcgtcgacaa aatcgagatg
     1321 gccgcgatag cccagcgaat agagcctctc gagctcggcc agcatctgcg tcggggtctt
     1381 ggtgcgcggc acgcggtcat aaagctctat gatgtcgcaa aactcgcaaa ggaacgggca
     1441 gccgcgcgag aactgcaccc cgatgtataa atactgcttg aacttcagca gatcgaagcg
     1501 cgggatcggc gtcttggtga cgtcggcctg gaatttcggc gcctcgaata taccagtgcg
     1561 ttccccacgc cgccaggcgg cgatgaactc gtcgataata ctctcggcct cgccgagcat
     1621 cataatatcg gcacgagcat aaatccttgg gctcgacgtt gggtcggggc cgccgaccgc
     1681 aaccggcttg ccggcacgct gcgccagctc gatgaccttc agcgaatcga attgctgggc
     1741 cagcatgcca ccggtcatga cgatgtcggc ccaggcgaca tctccgtcct gcagctcctc
     1801 ggtattgcgg ttgacgaggc gaatatccca tgacggcggc agcagcgccg ccagcgtgat
     1861 catgccgagc ggcgccgccg gaatttcgcg cccaggagct cgcaagcgcc gtcgtaactc
     1921 cagaacgact tgggattgaa tttcggaaag atcatcaaga cgttgcagga gtcggacaca
     1981 gtgcgtcatc ccctcgacga attcgttcta tctgacattt ccggattacc gaaattgcgc
     2041 gattttggcc ctctcaagcc tccgtggcaa caagggcgcc cgccaacagt tgccctggca
     2101 gagtagccca cgggctccag cacccgcgcc agctatccgc gcttctctcg atctcgcaca
     2161 agatattgta accgattcta gaatcgtccc aaatgctcgt aaaactgcga cctcctccgg
     2221 ggaatgcggt gtaattcaaa tgaggaagcg ctgtccgcga gggaccgaaa gtttgcagac
     2281 tccccgctgg agggaggtgg attcgaactt tccgttccgc gctttgaatg agcgtcgaac
     2341 cgcgccaatc tcgcgcggaa ccgggagacc gaatgcattg ctctcccggc ggctaccgat
     2401 agataaaatc acaagcttgg atgcccgatt tcgatacttg cgagccgtca gcatgagagg
     2461 agtgatgcaa agcggccttc gatgttatcg tcattacacc gattaaggca gccgtaaccc
     2521 aaaggcgtcg
//
LOCUS       DATLJT010000242         3391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3458515,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000242 DATLJT010000000
VERSION     DATLJT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3458515"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VM910_05520"
     CDS             <1..531
                     /locus_tag="VM910_05520"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HVI62038.1"
                     /translation="GFRAVPGAIVRRSAYIARNVVLMPSFVNLGAYVDEATMVDTWST
                     VGSCAQIGKRVHISGGVGIGGVLEPLQAEPVIVEDDCFIGARSEVAEGVIVRKGAVLA
                     MGVFLGASTKIVDRETGETFVGEVPEYAVVVPGTLPAKPLKNGQPGPSTACAVIVKRV
                     DEKTRAKTSINELLRD"
     gene            701..1855
                     /gene="dapE"
                     /locus_tag="VM910_05525"
     CDS             701..1855
                     /gene="dapE"
                     /locus_tag="VM910_05525"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HVI62039.1"
                     /translation="MTDALSIAQQLIRCPSVTPADAGALGVLENLLKAHGFEVHRITF
                     SEPGTADIDNLYARIGSEAPHITFAGHTDVVPPGDETAWSHGAFSGEVKDGFLYGRGA
                     VDMKGGIACSVAAVLQYLGDHSGKPKGSISFLITGDEEDVSVNGTIKLLEWVKARGEK
                     FDHCVLGEPSNQEELGDCIKVGRRGSQSGTLYVEGRQGHVAYPHRASNPVPDISRLIV
                     ALAEEPLDHGSAQFQPSNLEFTSVDVGNAASNVIPGRARAKFNIRFNDHFTQETLKKL
                     VDERLAKAAGNRIRAHIVWENSNSNVFLTKPGPFTDLAVAAIEEVTGRKPELSTTGGT
                     SDARFIARYCPVIEFGLLGQTMHQVDERAAVSDIEKLTKIYRGILDRYFG"
     gene            complement(2015..2752)
                     /gene="truA"
                     /locus_tag="VM910_05530"
     CDS             complement(2015..2752)
                     /gene="truA"
                     /locus_tag="VM910_05530"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVI62040.1"
                     /translation="MPRYKLTIEYDGTPFSGWQIQDNALTVQGALEAAVKAICGAEVR
                     VHGAGRTDAGVHALGQVAHCDIAKNFAPGRLRDGLNAHLRPNPIAVLAAEIVPETFEA
                     RFSAVKRHYLYRIRNTRANLALEVGRVWRVPRRLDAEAMHQAAQRLIGKHDFTTFRDT
                     ECQAKSPDKTLDQLDVTRAGDAVNIVTSARSFLHSQVRSMVGSLVWVGEGRWSAEDLS
                     AALAARDRAACGPVAPPQGLYLVRVDY"
     gene            complement(2872..>3391)
                     /locus_tag="VM910_05535"
     CDS             complement(2872..>3391)
                     /locus_tag="VM910_05535"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVI62041.1"
                     /translation="MKMDAGLDTGDVAMAERIAITDRMTAADLHDALARLGADLMVRA
                     LAALERGALQLTVQSGEGVTYAEKIDKAEARIDWNRPARAVLRHIHGLSPFPGAWSEV
                     AGDGEARIKVLRCELASSAGAAGAVLDDRLTIACGQGAIRIVELQRAGKAPMKAADFL
                     RGTPLEAGARFS"
BASE COUNT          634 a         1137 c         1002 g          618 t
ORIGIN      
        1 ggcttccgcg ccgtccccgg cgcgatcgtg cgacgctccg cctatattgc caggaacgtc
       61 gtgctgatgc cctccttcgt caatctcggc gcctacgttg atgaagcgac catggtcgac
      121 acctggtcga ccgtcggctc ctgcgcccag atcggcaagc gcgtgcacat ctcgggcggc
      181 gtcggcatcg gcggcgtgct cgaacctttg caagccgaac ctgttatcgt cgaggatgat
      241 tgcttcatcg gcgcgcgcag cgaagtcgcc gaaggggtga tcgtgcgcaa aggtgcggtg
      301 ctggcgatgg gcgtgttcct cggtgcctcg acaaagatcg tcgaccgcga aaccggcgag
      361 acctttgtcg gcgaagtgcc ggaatatgcc gtggtcgtgc cgggcaccct gccggcaaag
      421 ccgctgaaga atggccagcc cggcccctcc actgcatgtg cggtcattgt caagcgcgtg
      481 gacgagaaga cccgcgccaa gaccagcatc aacgagctgt tgcgggactg aacccggcgc
      541 cgcgaactca cggacgaact tccgaacgcg acgcgcatta gccaaatcat cgtgcgagtg
      601 ccgttttcga tttgtggttc atgtcgaagt cgcaagacgg gacatgaact tcagaacgcc
      661 ggcactagcc cagcgccggc cacgcatgtt aagcgggggc atgactgatg ctctctccat
      721 tgcccagcaa ctgattcgct gcccatcggt aactcctgcc gatgccggcg cgctcggcgt
      781 cctcgaaaac ctcctcaagg ctcacggctt cgaggtgcac cgcatcacct tcagcgagcc
      841 cggcaccgcc gacatcgaca atctctacgc tcgcatcggc agcgaggcgc cgcatatcac
      901 ctttgccggc cacaccgacg ttgtgccccc cggcgatgag accgcgtgga gccatggcgc
      961 gttctccggc gaggtgaagg acggtttcct ctatggccgc ggcgcagtcg acatgaaggg
     1021 cggcatcgct tgcagcgttg cggccgtgct gcaatatctc ggcgaccaca gcggcaagcc
     1081 gaagggatcg atctcattcc tgatcaccgg cgatgaggag gacgtgtctg tcaacggcac
     1141 gatcaagctc cttgagtggg tcaaggcgcg cggcgaaaaa ttcgatcatt gcgtgctcgg
     1201 cgaaccctcc aaccaggagg aattgggaga ctgcatcaag gtcggccgcc gtggctcgca
     1261 atccggcacg ctctatgtcg aaggacggca gggccatgtc gcctacccgc atcgcgcgtc
     1321 caacccggtg ccggatatct cacggctgat cgtggcgctc gccgaagagc cgctcgatca
     1381 tggcagcgcg caattccagc cctccaatct cgagttcacc tccgtcgacg tcggcaatgc
     1441 cgcaagcaac gtgatcccgg gccgggcgcg tgccaaattc aacatccgct tcaacgatca
     1501 tttcacgcaa gagacgctga agaagctcgt cgacgagcgc cttgcgaagg ctgccggcaa
     1561 ccgcatccgc gcccacatcg tgtgggagaa ttcgaactcc aacgtattcc tgaccaagcc
     1621 tggtccattc accgaccttg cggtcgctgc gatcgaagag gtcaccggcc gcaagccgga
     1681 gctctccacc accggcggca catcggacgc gcgtttcatc gcacgctatt gtccggtgat
     1741 cgaattcggc ctgctcggtc agaccatgca tcaggtcgac gagcgcgccg cggtgtcgga
     1801 tatcgagaag ctgacgaaaa tctatcgcgg gatcctggat cggtatttcg gctagagcag
     1861 gcaccggttt tccctcgaca aacgcggaac gcgtttgcac ggagatcatg ctctagagcg
     1921 cgatagcttt atgtcgaatc gccatcgcgc tctagtttat tttttgagca tgatcttttt
     1981 cggaaaaccg gtgtccactt ttccggatca tgctctaata atccacgcgc accaaataca
     2041 gcccctgcgg cggcgccacg ggaccgcaag ccgcacggtc acgcgcggcg agcgcggccg
     2101 acagatcctc ggcactccag cgcccctcgc cgacccagac cagcgagccc accatcgagc
     2161 gcacctgact atgcaggaac gatcgcgctg aggtgacgat gttgaccgca tcgccggcgc
     2221 gcgtgacgtc gagctgatcc agcgtcttat ccggggattt tgcctggcat tcggtgtcgc
     2281 gaaaggtcgt gaaatcatgc ttgccgatca ggcgctgcgc ggcctgatgc atcgcctccg
     2341 cgtcgagccg ccgcggcacg cgccagacac gtccgacctc gagcgcgaga ttggcgcgcg
     2401 tgttgcggat ccggtaaaga tagtgccgct tcaccgcaga aaatcgcgcc tcgaatgtct
     2461 cgggcacgat ctcggccgca agaaccgcga tcgggttggg gcgcaaatgc gcgttcagtc
     2521 cgtcgcgcag ccgacccggc gcaaaattct tcgcaatgtc gcaatgggcg acctgtccca
     2581 gcgcatgcac gccggcatcg gtgcgtccgg cgccgtgcac ccggacctcg gcgccgcaga
     2641 tcgctttcac cgctgcttcc agcgcgccct gcacggtcag cgcattgtcc tggatctgcc
     2701 agcccgaaaa cggcgtgccg tcatattcga tggtgagctt gtaacgtggc atcttcactc
     2761 gatggatgtc atcgtccgcg aaatcggacg atccaatagt ccatgacctc tcagttcaat
     2821 cgcctgactt tcggcgcttg ggatacgccg ccttcgcggg ttaccacagc ctcaagaaaa
     2881 acgagcccct gcctcgagcg gcgtgccgcg caggaagtcg gccgccttca tcggcgcttt
     2941 tccggcgcgc tgcaattcga cgatccggat tgccccttgg ccacaggcaa tggtcagccg
     3001 gtcgtccagc accgcacccg ccgcacctgc gctactcgca agctcgcagc gcaggacctt
     3061 gatccgcgcc tcaccatcgc ctgcgacctc gctccaggcg ccgggaaatg gcgaaagacc
     3121 atggatgtga cgtaacacgg cacgcgcagg cctgttccaa tcgatccgcg cctccgcctt
     3181 atcgattttt tccgcatagg tcacgccctc gccgctctgt acggtgagct gcaacgcgcc
     3241 gcgctcgagc gcggcgagcg cccgcaccat cagatcggcc ccgagccgcg ccaacgcatc
     3301 gtgcaggtcg gccgcagtca tccgatccgt gatcgcgatg cgctcggcca tcgccacatc
     3361 gccggtatcg aggcccgcat ccattttcat g
//
LOCUS       DATLJT010000243         1787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3917781,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000243 DATLJT010000000
VERSION     DATLJT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917781"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VM910_05540"
     CDS             complement(<1..872)
                     /locus_tag="VM910_05540"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI62042.1"
                     /translation="MNHPVASERFARHALGTRYEDLSDDAVLQAKTFILDTMGVGIAG
                     SSAAGADELSQVSAGWGEGAEACVWGRRRRVPAPTAAFLNGFQVHCQEYDCLHEKAVL
                     HAMATLLPATIAYAERMGGASGRELIAAVVVGIDVAVNLGVAAREGLKFFRPATSGGF
                     GATAAIARLGRFDLDTLCNAFGIQYGQTSGTMQAHVEGSIVLPMQIGFNSRAAIQSCD
                     MAASRLSGPRDVFEGQFGYMRLYEGSFQCGHTNSGTRGLCMWSIGAAFFNALDRGSSR
                     TCWKHRTARLRYFHV"
     gene            1076..1216
                     /locus_tag="VM910_05545"
     CDS             1076..1216
                     /locus_tag="VM910_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62043.1"
                     /translation="MPRLAAKVLREGSSCKFTKEGEVALRVRKVTDATGLSLPLPIWA
                     SA"
     gene            1539..1760
                     /locus_tag="VM910_05550"
     CDS             1539..1760
                     /locus_tag="VM910_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62044.1"
                     /translation="MARQIVMDTTGDTCHEFDPADPTAVAEAERRFKELTGAGFTAAV
                     RRGEGRSELIRSFDSAAEETLFFPRLKAG"
BASE COUNT          371 a          554 c          532 g          330 t
ORIGIN      
        1 acgtgaaaat atcgcagccg cgctgtcctg tgcttccagc aagtgcgtga acttccacga
       61 tccaaagcat tgaagaaagc agcaccaata ctccacatgc acaggccacg tgtgcctgag
      121 ttagtgtggc cgcactgaaa cgaaccttcg tacagccgca tgtatccgaa ctgtccctcg
      181 aacacgtcgc gcggacccga caatcggcta gccgccatat cgcaggactg tatcgccgcg
      241 cggctgttga agccgatttg catgggcaac acaatgctgc cttccacatg cgcctgcatg
      301 gtaccgctgg tctggccgta ttggatgcca aacgcattgc agagcgtatc aagatcgaaa
      361 cgcccgagcc gcgcgatcgc cgcggtcgcg ccaaagccgc ccgatgtcgc cggccgaaaa
      421 aacttcaagc cctcgcgcgc agcaacgccg agattcaccg cgacatcgat cccgaccaca
      481 accgcagcga ttaattcacg gcccgaagca ccgcccatgc gctccgcata agcgatcgtc
      541 gcaggcagca acgtagccat tgcatgcaac accgccttct cgtgcaagca gtcatattcc
      601 tggcagtgca cctggaaacc gttgaggaat gctgcggtcg gagcaggaac gcgcctgcgg
      661 cgcccccaca cgcaggcttc tgcaccctcg ccccagccag cgctgacctg cgagagttcg
      721 tctgcccccg cggccgatga cccggcaatg ccaacgccca tcgtgtcgag gatgaatgtt
      781 ttcgcctgga gaaccgcgtc gtcggacaga tcttcgtacc gtgttccaag ggcgtggcgc
      841 gcaaagcgct cggacgcgac cggatggttc atgcggcgct ccgcgagacg gcggaccata
      901 ccagatatct tggagcgatt acgccggtcg taagattacg ccctgtcggc aagccccctg
      961 gtcggcatac tgtcggggga cgttgcccac ggcgcggtaa tctgcctgtt cctgttcccc
     1021 ttcctgctga tcgcggtggt tttgttgcgc gcgctccggc gccgggagat ctgacatgcc
     1081 gcggcttgcc gcaaaggtcc tgcgcgaagg atcgtcctgt aagttcacga aagaaggcga
     1141 ggtcgccctg cgggtgcgca aggtgacgga cgcgactggg ttgagcttgc cgttgccgat
     1201 atgggcatcg gcatgacggc tcagcagctg gcaaagctga ctaaacaccc gatcgcctcg
     1261 cgttttggcg aaaacatccg tctcagctca gatttggcta acccgttgag caagagccaa
     1321 aattagcggg cggatgatga cggatgcgtc ggtttgccag gatgtcgcac gccgtcacac
     1381 cctgagcccg ccgcccgcga ccctggcagc cgataagaag cgacaattta agaccgtcca
     1441 tcgatccaga ttggacaagc gctgcagaat cgctcctttc ggggtcttgt ggccccagct
     1501 atgcttgcaa gggtagtgca cgagaaggag gggcatacat ggcaaggcag atcgtgatgg
     1561 atacgactgg cgacacctgc cacgaattcg atcccgccga tcccaccgcg gtggcggaag
     1621 ccgagaggcg gttcaaggag ttgaccggcg cgggcttcac cgcggccgtg cgcagaggtg
     1681 agggacgttc ggagctcatc cgaagtttcg attccgccgc cgaagagact ttgttctttc
     1741 ctcggctgaa ggctggttaa cgcttagatt ttggagaagg attgaac
//
LOCUS       DATLJT010000244         5230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1620490,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000244 DATLJT010000000
VERSION     DATLJT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620490"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VM910_05555"
     CDS             <1..1809
                     /locus_tag="VM910_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HVI62045.1"
                     /translation="GWIMHGTLGGMLAGGLVILPPMICMMALSYGYVTGGDSTIGEVL
                     LYGLKPAILVIVLEATIRVGRQVLRTRLMVALAGLAFVGTFFFNLSFVPVVVGAAVIG
                     FCAALVGSSAHPGAVHAQGAYGAFMGPAAKDIRQDDLPEHTRPTAGRLLRTGAFWLAL
                     WFVPVGVLVAALGLDNVFSQIAILFSKMAALSLGGPYAVNSYVALHAVEPYGWLTHEQ
                     ALDGLALAELAPGPVILFQQFVGFLAAYRNSGVLPPLAAATLGGLLSVWVISIPPFLW
                     ISLVGPFIERFRNNPHINTTLSAVTGGVLGVILTFAIRFGTRTIFTESVPISGFGLDF
                     SLPNFASADPWALAIAVVAAIAMFRFRLGMVATLAASCAIGTIPYLIDTIGLGSLRSG
                     LLGFVAGAAIGFPAGPVAVWCLHLRIQQRRAMASAIIAGSAIGDLIVASVFFVVTDLF
                     GGAITSLQMLKNPLFQGPVLILGGIALLFIVSRSVLLGLPQQDQTRDEQWTYVGAGLA
                     ALIALSASVTHPENLLAIGAVFAILGIGSDSGITLLAGFFIGTALTWFSTIELLCHLG
                     EKQGRQIMLRVMQSLCVLCIAAGFVQLARAANLLGI"
     gene            complement(1962..2861)
                     /locus_tag="VM910_05560"
     CDS             complement(1962..2861)
                     /locus_tag="VM910_05560"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI62046.1"
                     /translation="MAITVYPVTPNFAAEIGDVDLARPIDSTDLQAIKDAFTQHAVLI
                     FPDQHLSQDQHLDFARYFGPLETTIAVYRKDAPLRLRKEFADVSNLNPDSEVWGKESR
                     LRLFQLGNRLWHTDSSFKRLPARASLLYARSIPPVGGHTEFADERAAYDALPEETKRR
                     LDRLVAEHSIFNSRARLGFTNFSDDERRGMPPVPQVVVRTLPESGRKSLYLASHAGRI
                     FGMPEHEGCALIDQLVAHATQRQFVYTHRWRINDLVMWDNRCTMHRGTEFDDLRWKRD
                     VQRATVCDVANSCEQEGIEIAAA"
     gene            complement(3093..3683)
                     /locus_tag="VM910_05565"
     CDS             complement(3093..3683)
                     /locus_tag="VM910_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI62047.1"
                     /translation="MDEQSKLLRRSAIARILAERGDTLVVTGLGSPTYDCAAVGDHPL
                     NFYLWGAMGSAVTVGLGLALAQPKRRVLVVTGDGEMLMGLGALATAAAKKPDNLAIAV
                     IDNEHYGETGMQPTHTSAGVDLAGIALTCGFRQTATVRQVSELDAAVPMLHTAKGPVL
                     VVLKVSSARDPLVLPPWDGPFLKSRFRQALLGSQAA"
     gene            complement(3688..4212)
                     /locus_tag="VM910_05570"
     CDS             complement(3688..4212)
                     /locus_tag="VM910_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI62048.1"
                     /translation="MATDAQNWPNLIFEKLREFDVRHVTYVPDSGHARLIELCHAANE
                     IKPTVLTTEEEGIGILAGAWLGGERGVLLMQSSGVGNCINTLSLARVCAFPLLMIVTM
                     RGEWGEANPWQVPMGQIAADILRLAGAIVYEVTDPQAAAATVEAAARIAFNGNLTAAV
                     LLSQRMLGAKLFKD"
     gene            complement(4275..4985)
                     /locus_tag="VM910_05575"
     CDS             complement(4275..4985)
                     /locus_tag="VM910_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62049.1"
                     /translation="MPLLEVDRLYARYDYAPVVHGVSFSVDAGEIVSIFGRNGAGKTT
                     TLRTIMGWLRPSQGRIVFEGEAIEGMSPDRVFRKGIAFIPEDRRIFATLSVEENLILG
                     LFSRWQSGVEQRRRLDRVFDLFPRLRERRRQLGKTLSGGEQQMLAIGRALVGSPKLLL
                     VDEPSEGLAPMVVNEIFESLARMRSEGVALLLVEQNVRRAAAVSSSCYVMEKGRIIKH
                     GAPADVMIDEAIRQRLSV"
     gene            complement(4972..>5230)
                     /locus_tag="VM910_05580"
     CDS             complement(4972..>5230)
                     /locus_tag="VM910_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62050.1"
                     /translation="RLLMLDEPTQGMSPSETAEFDALIKSLTGRVTILLIEHDVDLVM
                     SLSDHVIVMHQGEKLFEGAPDHVRASPLVREAYLGVDHAAA"
BASE COUNT          941 a         1613 c         1596 g         1080 t
ORIGIN      
        1 ggctggatca tgcacggcac gctcggcggc atgctcgcag gtggcctcgt catcctgccg
       61 cccatgatct gcatgatggc gctgagctat gggtatgtca ccggtggtga ttccacgatc
      121 ggcgaggtgc tgctttacgg actcaagccg gccatcctgg tcatcgtgct cgaagcgacg
      181 attcgagtgg gaaggcaggt gctacgaacc cgactgatgg ttgcgctcgc gggcttggca
      241 tttgtcggga cttttttctt caatttgtcc ttcgtgcccg ttgtcgtcgg agccgccgtg
      301 atcggcttct gcgctgcgct tgtaggttct tcggcacatc ctggcgcggt gcatgctcaa
      361 ggcgcctacg gcgcgttcat ggggccggcc gctaaagaca tccgtcaaga tgatttgccg
      421 gagcacaccc ggccgaccgc gggtcgcctg cttcgcaccg gcgcgttctg gctcgcgctt
      481 tggtttgttc ccgtcggagt gctggtcgct gcgctcggcc tcgataatgt cttcagccag
      541 atcgcaatct tgttcagcaa gatggcggcg ctgtccctgg gtggccccta cgccgtcaac
      601 agctatgtcg cgctccacgc ggtagaacca tacggttggc ttacgcacga gcaggcactg
      661 gacggtcttg cgctcgccga gctggcgccg gggccggtca ttctgttcca gcagttcgtc
      721 ggtttcctcg cggcatatcg caattccggc gtgctgccac cgctcgcggc agcgaccttg
      781 ggcgggctgc tcagcgtatg ggtcatttcc atcccgccgt tcttgtggat ttctctcgtt
      841 ggcccgttca tcgaaagatt tcgtaacaac ccgcacatca acacgacgtt gagcgccgtc
      901 accggtggcg tcctcggcgt cattctaacc tttgcgatta ggtttggtac gcgcacgata
      961 tttacggagt cggtgccgat cagcggcttt gggctcgatt tcagccttcc gaatttcgca
     1021 agcgccgatc catgggcgct ggcgatcgcg gttgtcgccg ccatcgccat gttccgcttc
     1081 aggcttggaa tggttgcgac gttagcggca tcctgcgcca tcggaaccat tccctatttg
     1141 attgacacaa tcgggctagg gagcctgcgc agcggcctgc tgggatttgt tgccggcgcc
     1201 gcgattgggt ttccagccgg tccggttgcc gtttggtgtc tgcatcttcg catccagcaa
     1261 cggcgagcga tggcgtccgc catcattgcg ggcagcgcga tcggcgatct cattgtcgca
     1321 tcggtattct tcgtcgtcac cgaccttttc ggaggcgcca tcacttcgct gcagatgctc
     1381 aaaaatccgc tctttcaagg ccccgtgttg atcctgggcg ggattgcgct gctgttcatc
     1441 gtgagccgga gcgtgctcct ggggctgccg cagcaagacc agacgcggga cgagcaatgg
     1501 acctatgtcg gcgcgggact cgcggccctg atagcgttgt ccgcgagcgt aactcacccg
     1561 gagaatcttc tggcgattgg ggcggtcttc gccattcttg gaattggatc cgacagcggc
     1621 atcactctgc tggcagggtt cttcatcggg accgcgctca cctggttcag cacgatcgaa
     1681 ttgctctgcc atctgggcga aaagcaggga cggcagatca tgctgcgcgt gatgcagagc
     1741 ttgtgtgtgc tgtgcattgc ggcgggattt gtccaactcg cccgcgcggc gaacctcctc
     1801 ggcatctaaa cgccgctgcc acggcgatgc gatgcggttc aactctgcgg cgccgagcag
     1861 cagcggccga cggaaggtcc ggcagcagca gcgaaccggc tttcaggcac aaatgtgatg
     1921 aatacggttt tgtgtaagaa tgtcgtaacg cacgcaagcg gttacgcggc cgcgatctcg
     1981 atgccttcct gctcgcagga attcgccacg tcgcagacgg tggctcgctg cacgtcgcgc
     2041 ttccagcgca gatcgtcaaa ctcggttccc cggtgcatcg tacagcgatt atcccacatg
     2101 acgaggtcat tgatccgcca gcgatgggtg taaacgaatt gccgctgcgt ggcgtgcgcg
     2161 accaattgat cgatcaatgc gcagccctcg tgctcaggca tgccgaagat ccgacccgcg
     2221 tgggacgcga ggtaaagcga tttgcgtcct gattccggga gggtgcgaac gaccacctgg
     2281 gggaccggcg gcattccccg tcgctcgtca tcgctgaaat tggtgaagcc gagccgcgca
     2341 cgagagttga agatcgaatg ctcggccacc aggcgatcga gacggcgttt cgtctcctcg
     2401 ggaagcgcat cgtaggccgc acgctcatcg gcaaattcgg tatgtccgcc gactggcggg
     2461 atcgagcgcg cataaagcag cgatgcccgt gcaggcagcc gtttgaacga gctatcggtg
     2521 tgccaaagcc ggttacccaa ttggaacaag cggagtctgc tttccttgcc ccacacctcg
     2581 ctgtcgggat tcaggttgga aacgtcggca aactccttgc gtagacgcag cggcgcatcc
     2641 tttcgataaa cggcgatggt ggtctccagc gggccgaagt atctcgcgaa gtcgagatgc
     2701 tgatcctgcg agagatgttg atcgggaaag atcaagaccg cgtgctgcgt gaacgcgtct
     2761 ttgatcgctt gcaggtccgt ggagtcgatt ggtcgagcca agtcaacgtc gccgatttcg
     2821 gcagcaaagt ttggcgtgac cggataaacg gtgatggcca tgatcatgcc ctcggcgcac
     2881 gagatttcgc cggtgttaga agaattgaat catggcggac caacgcttgc aatcgactcg
     2941 gaggaaattt ttaagccgga gcgaccagga gggaaagcgg gtttcacctt acgcgccatc
     3001 ggcgcaacgg gcgggtgtgg cgctgttaca tcaatccgta tcgtccttcg aaccgcggca
     3061 gccccctaaa tccggtggat gcatttggct cttcaggcgg cctgcgaccc gagcagggcc
     3121 tggcggaaac gcgacttgag gaacggtccg tcccacggtg gcaacaccag cgggtcgcgc
     3181 gcggacgaga ccttcaggac cacgagcacc ggacccttgg ccgtatgcaa catcggcacc
     3241 gcggcgtcga gctcgctcac ctgcctcacg gtcgcggtct ggcggaagcc gcaggtgagc
     3301 gcgatgcccg caagatcgac acccgcgctg gtgtgggtcg gttgcatgcc ggtttcgccg
     3361 taatgctcgt tgtcgataac ggcgatcgcg aggttatccg gttttttcgc cgccgcggtc
     3421 gccaacgctc ccaaccccat cagcatttcg ccgtcgcccg taacgaccaa gacgcggcgt
     3481 tttggctgcg ccagagccag tcccagtccg accgtgaccg cgctccccat cgcgccccag
     3541 agataaaaat tgagcggatg atcgcccacc gccgcgcaat cataggtcgg cgagccgaga
     3601 cccgttacca cgagcgtatc gccgcgctcg gccaatattc gggcgatggc gctgcggcgc
     3661 agcagcttgc tttgttcgtc catgggttca gtccttgaaa agcttggcgc cgagcatgcg
     3721 ctgcgagagc aggaccgccg cggtcaggtt gccattgaag gcgatgcgcg cggccgcctc
     3781 cacggtcgcg gccgccgctt gcggatcggt cacttcgtaa acgatcgcgc ccgccagccg
     3841 caggatgtcg gccgcgatct gacccatcgg tacctgccaa ggattggctt cgccccactc
     3901 gccacgcatg gtgacgatca tcaacagggg gaaagcgcac accctcgcaa gcgacagcgt
     3961 gttgatgcag tttccgacgc cgctgctttg catcagcagg acaccccgct cgccgccaag
     4021 ccaggcgccg gccaaaatgc cgatgccctc ctcttcggtg gtgagcacgg tcggctttat
     4081 ctcattggcg gcgtggcaca gctcgatcag ccgcgcatgt cccgagtcgg gaacgtatgt
     4141 gacatggcgg acatcgaatt cgcgcagctt ttcgaagatc aagttcggcc aattctgcgc
     4201 atcggtggcc atgatggtgc cggtcgcatc gttgttcact cgtccgtgtc gccgagtggc
     4261 catttgccgt cctttcaaac agacaaccgt tgccggatgg cttcatcgat catcacgtcc
     4321 gcaggtgcac catgctttat gattcgcccc ttttccatca cgtaacagga actgctgaca
     4381 gcagccgcac ggcggacgtt ctgctccacg agcagcagcg cgacgccctc gctgcgcatt
     4441 cgggcaagcg attcgaaaat ttcattgacg accatggggg ctaagccttc ggacggttca
     4501 tccaccagca agagctttgg cgatccgacc aaggcgcggc cgatcgccag catctgctgc
     4561 tcgccgccgg aaagagtctt gccgagctgg cggcgtcgct cgcgcagccg cgggaataga
     4621 tcgaacacgc gatcgagccg gcggcgttgt tcgacgcctg actgccatct gctgaatagt
     4681 ccgaggatga gattctcttc gacgctcaac gtcgcaaaga tgcgacggtc ttccggaata
     4741 aaggcgatgc ctttccggaa cacgcgatcg ggcgacattc cttcgatcgc ctctccctcg
     4801 aatacaattc tcccctgaga cggacgcaac caccccatga tcgtgcgcaa tgttgtcgtc
     4861 ttgccggcgc cgttgcgccc gaaaatactg acgatctccc ccgcatccac gctgaacgaa
     4921 acgccgtgca ccaccggggc ataatcgtag cgcgcataga gccgatcgac ctcaagcagc
     4981 ggcatgatcg acccccagat acgcttcccg gacgagcgga ctggcgcgca cgtgatcggg
     5041 cgctccctcg aacagctttt caccctgatg catgacgatg acgtggtcag acaagctcat
     5101 gaccagatcg acgtcgtgct cgatcaaaag gatggtgacg cggcccgtca gcgatttgat
     5161 caatgcgtcg aattcagccg tttcggaggg cgacatccct tgcgtgggct cgtcgagcat
     5221 cagcagcctg
//
LOCUS       DATLJT010000245         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2539524,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000245 DATLJT010000000
VERSION     DATLJT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539524"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="VM910_05585"
     CDS             <1..1222
                     /locus_tag="VM910_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62051.1"
                     /translation="EASYLLERMMETAGRQLKVDPADLRHKNFIRQFPYQTPVIMAYD
                     TGDFVASLDAAKKAIDYAGFPARKAKAKSEGKLRGIGVSCYIEACGIAPSKAVGSLGA
                     GVGLWESAEVRVNPVGTIEVLTGSHSHGQGHETTFCQLVADRLGISINQVQIVHGDTD
                     KVQFGMGTYGSRSAAVGLTAILKAMEKVEAKAKKIAAHALEASEGDIVIENGEFKVTG
                     TDKKIALPMVALAAYTAHNLPDGMEPGLKESAFYDPTNFTFPAGAYICELEVDPATGK
                     TTFINFVAADDFGRLINPMIVEGQVHGGLAQGIGQALLEGAVYDENGQLVTASFMDYT
                     MPRADDLPSFKLSHTTTLCPGNPLGIKGCGEAGAIGASAAVINAITDAIGNNKLEMPA
                     TPDRVWRAIHGKAS"
     gene            1305..2102
                     /locus_tag="VM910_05590"
     CDS             1305..2102
                     /locus_tag="VM910_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HVI62052.1"
                     /translation="MYQTTYHCPSSVDEATGLFAKGKEAKYLAGGQTLIPVMKQRLAS
                     PSDVIDLGKIKELVGIDAAGDTVTIKAATTHYDVASSAAVQKAIPALAYLASVIGDPA
                     VRHRGTIGGSLANNDPSADYPSAVLALGATVKTNKRSIADSFFQGLFATAIEDGEIIT
                     AVSFPVPAKAGYAKFPHPASRFALTGVFVAKTKAGDVRVAATGASQNGVMRVPAIEAA
                     LKANWSAGALDSATIPASGLMNDIHGSSEYRANLIKVMAQRAVTAAG"
     gene            2257..>2903
                     /locus_tag="VM910_05595"
     CDS             2257..>2903
                     /locus_tag="VM910_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI62053.1"
                     /translation="MLDKPAPQSSVRASGPLAGFRIVEFAGIGPGPFACMMLADMGAE
                     VVTLDRVGAKKSMKSVAGRGRKVVELDLKDKGAIAQVLDLLASADALIEGFRPGVMER
                     LGLSPDVVLARNPKLVYGRMTGWGQEGPLAHAAGHDINYISLTGALAAIGPEEKPVPP
                     LNLVGDFGGGALYLVVGVLAALLESTRSGKGQVVDTAMCDGAASLMSMFFDMAAIG"
BASE COUNT          573 a          947 c          900 g          483 t
ORIGIN      
        1 cgaagcaagc tacctgctcg aacgcatgat ggagaccgcg gggcggcagc tcaaggtcga
       61 tcccgccgac ctccggcaca agaacttcat ccggcagttc ccctatcaga cgcccgtcat
      121 catggcctat gacaccggcg atttcgtcgc atcgctcgat gccgcgaaga aagcgatcga
      181 ttatgccggc ttcccggcgc ggaaagcgaa ggccaagtcc gagggcaagc tgcgcggcat
      241 cggcgtctcc tgctacatcg aagcctgcgg catagcgccg tcgaaagcgg tcggcagcct
      301 gggcgccggc gtcggcctct gggaatctgc cgaggtgcgc gtcaatccag tcgggactat
      361 cgaggttctg accggctcac acagccacgg ccagggccat gagacgacgt tctgccagct
      421 cgttgccgat cgactcggca tctccatcaa ccaggtgcag atcgtccatg gcgataccga
      481 caaggtgcag ttcggcatgg gcacctatgg ctcgcgctcg gcggccgtcg gtcttaccgc
      541 catcctgaaa gcgatggaga aggtcgaggc caaggcaaag aagatcgcgg cgcatgcgct
      601 ggaagccagc gaaggcgaca tcgtcatcga aaatggcgag ttcaaggtca ccggcaccga
      661 caagaagatc gcgcttccga tggtcgcgct cgccgcatac accgcacaca atttgcccga
      721 cggcatggag ccggggttga aggagagcgc gttctacgac ccgaccaact tcaccttccc
      781 ggcaggcgcc tatatctgcg agctcgaggt cgatccagcg accggcaaga ccactttcat
      841 caacttcgtc gccgccgacg atttcggccg gctgatcaat ccgatgatcg tcgaaggtca
      901 ggtccatggc ggcctggcgc agggcatcgg tcaggcgctc ctggagggcg cggtctatga
      961 cgagaacggc cagctcgtga ccgcatcgtt catggactac accatgccgc gcgccgacga
     1021 tctgccgtcg ttcaagctca gccataccac gacactctgc ccgggcaacc cgctcggcat
     1081 caagggctgc ggcgaggccg gcgcgatcgg cgcgtccgcc gccgtgatca acgcgatcac
     1141 ggatgcgatc ggcaacaaca agctcgaaat gcctgcgaca cccgaccgcg tctggcgcgc
     1201 gattcacggc aaggcgtcgt aattgttttt cctctccctt tcagggggag gtggagagaa
     1261 aagggccaac ggctgcgatg acgcgcagcg tccaggagca tcccatgtat cagaccactt
     1321 atcattgccc ctcctcggtc gacgaagcca caggcctctt tgccaagggc aaggaagcca
     1381 aatatctcgc cggcggccaa acgctgattc ccgtcatgaa gcagcggctc gcctcgccgt
     1441 ccgacgtgat cgacctcggc aagatcaaag agctggtcgg catcgacgcc gccggcgaca
     1501 cggtgacgat caaggcagcg acgacgcatt acgacgttgc gtcgagtgcg gccgtgcaaa
     1561 aggcgatccc ggcgctcgcc taccttgcct ccgtcatcgg tgacccggcc gtgcgccatc
     1621 gcggcaccat cggcggctcg ctcgccaaca atgatccttc cgccgattac ccgagcgcgg
     1681 tgctcgcgct gggcgcgacc gtcaagacca acaagcgttc gatcgcggac agcttctttc
     1741 aaggtctgtt cgcgaccgcg atcgaggacg gcgagatcat taccgccgtc tccttcccgg
     1801 ttccggccaa agccggctac gcaaagttcc cgcatcccgc ctcgcgcttt gcgctgaccg
     1861 gcgtgttcgt cgccaagaca aaggcaggcg atgtgcgagt ggccgcgacc ggcgcttcgc
     1921 agaacggcgt catgcgggtt cccgccatcg aagccgcatt aaaggccaac tggtcggccg
     1981 gcgcgctcga cagcgccacg ataccggcga gcggcctgat gaacgacatc cacggctcct
     2041 ccgagtaccg cgccaacttg atcaaggtaa tggcgcagcg cgcggtgacg gccgcgggct
     2101 gatagccact caacacaaga tgccacatat gcggcgcgca gaccgttgcg cgccgcatgc
     2161 attttaccgc agccgtcgca gacaaaacgc gcttgtcagt gagagcgcaa ggcgagacaa
     2221 tttccgtcaa aggaaaatct cgaaggcaaa gcaaccgtgc tcgacaaacc agctccgcaa
     2281 agctccgtcc gcgcttccgg cccacttgcc ggtttccgca ttgtcgaatt cgccggcatc
     2341 ggtccgggtc cgttcgcctg catgatgctc gcggacatgg gcgccgaggt cgtcacgctc
     2401 gatcgcgtcg gcgcaaagaa aagcatgaag tcggtcgcgg gccgcggccg caaggtggtc
     2461 gaactcgatc tgaaagataa aggcgcgatc gcgcaggtgc ttgatctgct cgcaagcgca
     2521 gacgcgctga tcgaaggctt ccggccgggt gtgatggaac ggcttggcct cagccccgat
     2581 gtcgtattgg cgcggaaccc gaaactggtc tacggccgca tgacgggttg gggccaggaa
     2641 ggtccgctcg cgcatgccgc cggtcacgat atcaactaca tctcgctgac cggcgcgctc
     2701 gcagcgatcg gccccgaaga gaagccggtg ccgccgctca acctcgtcgg cgatttcggc
     2761 ggcggggcgc tctatctcgt ggttggcgtg cttgccgcac tattggaatc cacgaggtcc
     2821 ggcaagggac aggtggtgga caccgcaatg tgcgacggcg cggcatcgct gatgtcgatg
     2881 ttcttcgaca tggccgcgat cgg
//
LOCUS       DATLJT010000246         5079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_931220,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000246 DATLJT010000000
VERSION     DATLJT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931220"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..835
                     /locus_tag="VM910_05600"
     CDS             188..835
                     /locus_tag="VM910_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI62054.1"
                     /translation="MLKVWGRRNSFNVQKVLWLIGELGLAHEHIPAGGSFGRLDEPSF
                     RALNPHGRVPVIEDGDLAVWESHAILRYLAARYDRGGLWSADPAERSRVDGWMDWSQA
                     TLQPDFLMGIFWGYYRTPQSQRDWPAIRKNLARCADHFRLLDDVLATRPFLAGDRFSL
                     ADIPAGTSLYRYFELDIERPAVANVTAWYERLRERPAYREHVMIPFVDLRGRLEP"
     gene            902..1948
                     /locus_tag="VM910_05605"
     CDS             902..1948
                     /locus_tag="VM910_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62055.1"
                     /translation="MWAIVALLVLPAAVLLFVSIIAYDILQHPERHQNTRRIWNVIAT
                     DLYYEYEANLWQNQKGCVQYDDKLLYKPSSGCHFVNKEFSTDLTFSEDGRLTTPSRAP
                     ASRRPLIFAGDSETMGWGVNDGETFASLIAASIEAPVLNLGVASYGTVREIMRIRMQR
                     RFQDANCIVIQYSWNDFDENSVFLARGALPPPTRRRFQQLVSDYGKRDVGFQDVMRRT
                     FDVMWNNPLAFFLNILGWHDFPLEDDDPMNMNGTPREETEDTKAFLAVLAAFPELNGK
                     KIFVMGPGRFISALSRETAPANVFPISVDLNNGASYTLDMHPNKQGHRDIAAQILDEL
                     KRTDEGRQCLAGEP"
     gene            complement(2043..2471)
                     /locus_tag="VM910_05610"
     CDS             complement(2043..2471)
                     /locus_tag="VM910_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62056.1"
                     /translation="MWFFNQKRADQVAQSISQLIHALSAGRENHGPTGPERLLRELAT
                     LLSEDTIIGQRVVKLLMATTTKAKFLVSLATTPDHSSHRLTFDGRGVYSGFSLACFLP
                     PRTVMIDLHPTAVGQHARLLYAACKAMPNVVERRDFRWDR"
     gene            2682..3131
                     /locus_tag="VM910_05615"
     CDS             2682..3131
                     /locus_tag="VM910_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVI62057.1"
                     /translation="MVGQTARTAKERLVGSWTLTSLTAGEGADRTLPYGPNPTGSMMV
                     DANGRFMITVVRSDLPNFASNNRMVGTPDENNSVVQGSIAYYGTYTIDEATGVITVKV
                     EGSTFPNFTGGTQTRILSFNGDDEVTYLNPTPSHGGEPAKVTYRRAK"
     gene            3223..4200
                     /locus_tag="VM910_05620"
     CDS             3223..4200
                     /locus_tag="VM910_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62058.1"
                     /translation="MEPIFALSMALVAILGSIGSAPAQDYPSRPITMIVPYPAGGLFD
                     ALARILAEPMRGALAQSVVIENVGGAGGSIAVGRVARAAPDGYTLAIGSADQFVVNAA
                     IYPLPYDVVKDFEPVALLISGPILIVSKTAVPAKNLKELMAWLKANHAQVAQGHNGSG
                     GAQHLCGIELQRIVGASWQFVPYRGAAPALQDVVGGRVDVMCPSPASSLAMVQSGLLR
                     AYAVTDATRLASAPDIPTVDEAGFPQLHISVWGGLFVPKGTPQSVIAKLNSAAATALA
                     DPAVRRQLANIGQEVVPREQQTPEALSAVQKADIEKWWPIIKAANIKAE"
     gene            complement(4289..>5079)
                     /locus_tag="VM910_05625"
     CDS             complement(4289..>5079)
                     /locus_tag="VM910_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62059.1"
                     /translation="QQVVIENRGGAGTNIGNEMVARSDPDGYTVLFATASLAVNRSLY
                     SSLSYDPIADFAAVSLVSRFSLFMFVPNSLPTKSVMDFVAYAKAHPGKLTLASPGTGS
                     TPHLAGELLKQMAGIEMTHVPYRGASPALNDLIPGRVDCYFGSGVLLENMRSGQLRGL
                     AVTGAKRDPVAPELPTMAEAGVPGYEVSSWHGLFVPARTPPEIVRKMSVDTIAALADP
                     VVKSKLEQAGYMVVGSSPNELKTLLKSEVDKWSAVIETVRIKIN"
BASE COUNT         1000 a         1535 c         1562 g          982 t
ORIGIN      
        1 cttttcagcg ctgatggaga cgatggcgct cgcctcgggg atgaagcttc aaagcgagaa
       61 caagaacgtg cccaccggat tggagagctg atgccatcgg ttctttcgac ggcattatca
      121 cggccatcaa accccggcgg acttagcgga gcgggataac ggcgccagcg gggagaaggg
      181 gtcgtccatg ctcaaggttt ggggacgccg caactccttc aatgtccaga aagtgctttg
      241 gctcatcggc gagctcggtc tcgcccacga gcacatcccc gccggcggca gcttcggcag
      301 actcgacgag ccgtcgtttc gggcgttgaa cccgcacgga cgcgtaccgg tgatcgaaga
      361 tggcgatctc gcggtgtggg aatcccacgc cattctcagg tatctggcgg cccgctacga
      421 tcgcggcggc ttgtggtctg ctgacccggc cgagcggtcg cgcgtcgacg gctggatgga
      481 ctggtcgcag gcgacgctcc agcccgattt cctgatgggg atcttctggg gctactatcg
      541 cacgccgcaa tcgcagcgcg actggcctgc gatccgcaag aatctcgcgc gctgcgccga
      601 ccattttcgg ctgctggacg acgttctggc aacgcggccg ttcctggctg gtgataggtt
      661 ctcgctggcg gacattcccg ccggcacgtc gctttatcgc tatttcgagc tcgatatcga
      721 acgtcctgct gttgcgaacg tgaccgcctg gtatgagcgc ctgcgcgaac ggccggcgta
      781 ccgcgaacac gtcatgatcc cgtttgtcga cttgcgcggc cggctcgaac cctgagcgtg
      841 cgaaggctga cccgtggccg aagctttgcc ccgcatcaag ctcgtattct ttcacgtcct
      901 catgtgggcg atcgttgcgc tgctggttct acccgcggca gttctgctgt tcgtgtccat
      961 cattgcatac gacatactcc agcatccgga gcgtcatcag aacacgcgca gaatttggaa
     1021 cgtcatcgcc accgatcttt attacgagta cgaggccaac ctttggcaga accagaaggg
     1081 ctgcgtgcag tacgacgaca agctgctgta taagccctca agcggctgtc acttcgtcaa
     1141 caaggaattt tcaaccgacc tcaccttctc ggaagacgga cggctgacga cgccctcacg
     1201 cgcgcctgcc tcgcgacggc cgctcatttt tgcaggcgat tccgaaacaa tgggatgggg
     1261 ggtcaacgac ggcgagacgt tcgcgtcgct cattgccgcc agcatcgagg cgccggtttt
     1321 gaatttgggc gtggcaagtt acgggacggt cagagagatc atgcgcatcc gcatgcaacg
     1381 gcgttttcaa gacgccaatt gcatcgtcat tcaatacagc tggaacgatt tcgacgagaa
     1441 cagcgtattt ctagccaggg gcgcgttacc gccccccacg cggagaagat ttcaacagct
     1501 ggtgtcggac tacggcaagc gcgacgtcgg cttccaagat gtcatgcgac ggaccttcga
     1561 cgtcatgtgg aataatccgc ttgcgttttt tctaaatatc cttggatggc atgacttccc
     1621 gttggaagat gacgacccca tgaatatgaa tgggactccc agagaagaga ccgaagatac
     1681 gaaggcgttc ctggccgtcc tggcggcttt ccccgagttg aacggcaaaa aaatcttcgt
     1741 gatgggccct ggccgcttca tctccgcgct aagccgcgaa acggcgcctg caaacgtgtt
     1801 cccgatctcg gtcgatttga acaacggagc ctcgtacacg ctcgacatgc atcccaataa
     1861 gcaggggcat cgggacatcg ccgcgcaaat attggatgag ctcaagcgga cggacgaagg
     1921 caggcaatgc ctagcgggtg aaccctgacc gagcgcgatg taaccgccgg tccgcacgtc
     1981 ctgcgaattc acctcgaggc gttatgcggc gtcggcagcg gacgcgcgca cccgcgataa
     2041 tttcagcgat cccagcgaaa gtcgcggcgt tcgaccacgt tgggcatggc cttgcaggcc
     2101 gcatacagca ggcgtgcgtg ctgtccgacc gcggttggat gcaagtcgat catgaccgtt
     2161 cgcggcggca ggaagcaggc gagcgaaaat ccggagtaca cgccgcggcc gtcgaaggtc
     2221 agcctgtggc tggaatgatc cggcgtggtc gccagactga ccaggaactt tgctttggtg
     2281 gtcgtcgcca tcagcagctt cacgacgcgc tggccgatga tcgtgtcctc gctgagcaag
     2341 gtcgcaagct cgcgcaggag ccgttccgga cccgtcgggc catgattttc tcgcccagca
     2401 gagagcgcgt ggatgagctg gcttatggac tgggcgacct ggtctgcccg tttctggttg
     2461 aaaaaccaca tgacacgaac tcaccccact ccgcggcgag cgctcgccgc gccgccccct
     2521 ctcgcaggtc gaaaactagc gtcggaggtc cgccctcggc aaccgcgcga ggtctgaaag
     2581 ttgaccgctg tgacattttt gcaaaatgat gggccaaccc cgtaggtcta agctatggga
     2641 aatggctcga ctgagccgtt tcttgccttc cggaggacgg tgtggtgggg caaacggcaa
     2701 ggaccgccaa ggagcggctc gtcggatcgt ggacgctcac ctcgctcacg gccggtgagg
     2761 gcgccgatcg aaccttgcct tatggtccca atccgacagg ctccatgatg gtcgacgcca
     2821 acggccgctt catgatcacg gtcgtgcggt ccgatcttcc gaactttgcc tccaataacc
     2881 gcatggtcgg cacgccggac gagaacaatt cggtggtgca aggcagcatc gcctattatg
     2941 ggacctacac catcgacgag gcgacgggcg tgatcaccgt caaggtcgaa ggaagcacgt
     3001 tcccgaattt caccggcggc acgcaaacgc gcatcctctc gttcaacggc gacgacgagg
     3061 tgacctatct caaccccacg ccgtcgcatg gcggggagcc ggccaaggtg acgtatcggc
     3121 gcgccaagta gcgccaatgt ttcgcgtacc agagtgagcg gccgacaaga gccgacggtc
     3181 acccacaaac tgagggagca gcccgtgcgt aggatgaaac gtttggaacc aatcttcgcg
     3241 ctcagtatgg cgctggtcgc aattttggga tcgatcggaa gcgccccggc gcaggactat
     3301 ccatcgcgtc cgatcacgat gatcgtgccc tatcccgcgg gtggcctgtt cgatgcgctc
     3361 gcacgtattc tggccgaacc catgcgggga gccctcgcgc aatcggtggt gattgaaaac
     3421 gtgggtggcg ccggcggcag catcgccgtt gggcgcgtcg cgcgcgcagc gccggacggt
     3481 tacacgctcg ccatcggctc cgcagatcag ttcgtcgtca atgctgcgat ctacccgctc
     3541 ccatacgatg tggtgaagga cttcgaaccg gtcgcgctgc tgatcagcgg ccccatcctg
     3601 atcgtcagca agaccgccgt cccggcgaaa aatctgaaag agttgatggc atggctgaag
     3661 gcaaaccatg cccaggttgc gcagggtcat aatggttcgg gcggagcaca gcacctctgt
     3721 ggcatcgaat tgcaacgcat cgtcggtgca agctggcaat tcgtgcccta tcgcggcgct
     3781 gctccggcgc tgcaggacgt ggtcggcgga cgcgtcgatg tgatgtgccc ttcgcccgcg
     3841 agttcattgg caatggtgca gagcggcctg ctgcgagctt acgccgtcac ggacgccacc
     3901 cgtttggctt ccgcgccgga tatcccgacg gtggacgagg cgggatttcc gcagttgcac
     3961 atttcggtct ggggcgggct gttcgtcccc aaagggacgc cccaaagcgt gattgccaag
     4021 ctcaattctg ccgccgcgac cgcgttggcc gatccggcgg tgcgtcggca gctggcaaac
     4081 atcgggcagg aggtggtgcc gcgcgagcag cagacgccgg aggccctcag cgcggttcaa
     4141 aaagccgaca tcgagaagtg gtggccgatc attaaagcgg cgaacatcaa agcggaatga
     4201 atgctgctcg ttgcggccgc ttgacgcggt cctttcgccg cgatcacatc acacatttct
     4261 cagaagtaga ctccgtgcgc cattggtcct aattgatctt gatccggacc gtctcgatga
     4321 cggcgctcca tttatcgact tccgatttga ggagagtttt aagctcattg ggcgaagagc
     4381 ccaccaccat atagccggct tgttccagct tgctcttgac cacgggatcg gcgagagccg
     4441 cgatggtatc gacactcatt ttcctgacga tttcaggcgg cgtcctggcc ggcacgaaca
     4501 agccgtgcca cgacgacacc tcgtaacccg gaacgccggc ctcggccatc gtgggcaatt
     4561 ccggcgccac cggatctctt ttggcgccgg taaccgcaag tccacgcagc tggcccgacc
     4621 gcatattttc gagcagcacg ccgctaccga aataacagtc cacgcggccg gggatgaggt
     4681 cgttgagggc gggtgacgcg ccgcgatagg gcacatgcgt catctcgatc ccggccattt
     4741 gcttaagcag ctcgccggcc aaatgcggcg tgctgccggt gccgggggat gccagtgtga
     4801 gcttgcccgg atgcgccttt gcataggcca caaaatccat gaccgatttg gtcggtagcg
     4861 agttcggcac aaacatgaac aatgaaaacc tggaaactag ggagactgct gcaaaatccg
     4921 caatcggatc gtagctgagc gagctgtaga gactgcggtt gactgcgagg gatgcggtgg
     4981 caaacaacac cgtgtagcca tccggatcgg accgggcgac catttcattg ccgatattgg
     5041 tgccggcgcc tcccctgttc tcgatgacga cttgctggc
//
LOCUS       DATLJT010000247         5223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_931253,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000247 DATLJT010000000
VERSION     DATLJT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931253"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VM910_05630"
     CDS             complement(<1..305)
                     /locus_tag="VM910_05630"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473095.1"
                     /note="Conjugates Arg from its aminoacyl-tRNA to the
                     N-termini of proteins containing an N-terminal aspartate
                     or glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HVI62060.1"
                     /translation="MTQHSRDTPQFYLTAPSPCPYLPGRHERKVFTHLVGDKAGDLND
                     LLTHGGFRRSQSIAYRPACDQCRACVSVRVIANEFRPSRNLRKVLARNADIVGEQRSA
                     "
     gene            complement(386..937)
                     /locus_tag="VM910_05635"
     CDS             complement(386..937)
                     /locus_tag="VM910_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVI62061.1"
                     /translation="MSYTGSGNTGDGWQGAGDPTYQHAFDPYVEPDLFRGVAMRRMIA
                     FIIDMIVLSIPIALAILFIAVFGLVTLGLGWALFWLVSPASIIWALIYYGASIGGSHS
                     ATIGMRMMDLELRTWYGAPGYFLLGAMHAVLFWISISVLSPFVLLIGPFNARRRLLHD
                     FVLGTVIINNSVRTHVAQSARTY"
     gene            complement(1049..2122)
                     /gene="hemB"
                     /locus_tag="VM910_05640"
     CDS             complement(1049..2122)
                     /gene="hemB"
                     /locus_tag="VM910_05640"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVI62062.1"
                     /translation="MAIKFGRPIEMRDGPRRDSATSAAPALDLTVRPRRNRKAEWARR
                     LVRENVLTSDDLIWPLFVADGHNKRTAIASMPGVDRLSVDQAVREAERAMKLNIPCIA
                     LFPYTDQSLRDEQGSEAVNPDNLVCQSVRAIKKEFPDLGILCDVALDPFTSHGHDGLL
                     EDGRILNDETVAVLVRQALVQADAGCDIIAPSDMMDGRVGAIREALDRAGFLDVQIMA
                     YAAKYASAFYGPFRDAIGSAKTLTGDKRTYQMDSGNSDEALREVELDIAEGADMVMVK
                     PGMPYLDIVRRVKDTFGMPTFAYQVSGEYAMIAGAANNGWIDGERAIMESLLGFKRAG
                     ADGVLTYFAPLAAEKLAAQGRTA"
     gene            2310..2765
                     /locus_tag="VM910_05645"
     CDS             2310..2765
                     /locus_tag="VM910_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HVI62063.1"
                     /translation="MSDTSPREAARDAMSAALSSERIDGNENVWTRRLVLFLRIMAVL
                     SILKGLYHWAQVTGFVGGEEEAFENQPMAWQAATVYFAVIELVAAVGLWLATPWGAVV
                     WLTTVVSMAVIELMFPGIYGGSLLVVAGEALMLTAYLALAWMAARERPP"
     gene            3109..3624
                     /locus_tag="VM910_05650"
     CDS             3109..3624
                     /locus_tag="VM910_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI62064.1"
                     /translation="MMKAVASAVETAERAPGQSQVQALYLEALTLVERLHRRLLDVIK
                     DEFDRRGRSDINSVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNLKKLVELGFLDH
                     QRSRVDRRSVRIRLTPQGQEIRRVVEALYQKHVKTVEQVGGISNEEFATLNKSLHRLE
                     RFWTDQILYRL"
     gene            3805..4029
                     /locus_tag="VM910_05655"
     CDS             3805..4029
                     /locus_tag="VM910_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62065.1"
                     /translation="MLVERGVRVLNTEVVGDAYAIASNYLRRAGAISANVAPNERLLG
                     IIVRLFKDGEINKIRLANQAIAEFEAAETN"
     gene            4050..4271
                     /locus_tag="VM910_05660"
     CDS             4050..4271
                     /locus_tag="VM910_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62066.1"
                     /translation="MEAAIDRIMQTYDLLMNRTPEATAVAREKVTQYVNTLFEAGERD
                     PHRLAVCGLAYLRELDGLTDPVKAGYTGL"
     gene            complement(4353..5138)
                     /locus_tag="VM910_05665"
     CDS             complement(4353..5138)
                     /locus_tag="VM910_05665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI62067.1"
                     /translation="MGRLEGKSVIITGAGSGIGRAASLLFTREGAKLIAVDRAEGVKE
                     IVEIVRKAGGTAEAVQADAGSEKDVIAFIDKALSAYGKLDAIWANAGVSGGLVPLAEQ
                     TVEHWQEVLRINLIGPFLAIKHSIPHMTKQGSGSIVCTASVAGLKSGASGHPYGASKA
                     GVISLVQITAYSLSGTGVRINAVCPGLIETGMTKPIFDRAKDRGTQDKIGQLNPLKRP
                     GQPQEIAAMGLFLASDEASYVNGQAFPVDGGLTASMPYTGKPI"
BASE COUNT         1028 a         1580 c         1601 g         1014 t
ORIGIN      
        1 gcgctgcgct gctcgccgac gatgtcggcg ttgcgggcca ggacctttcg aagattgcgc
       61 gagggacgga attcgttggc gatcacccgc acggagacgc aggcccggca ctggtcgcag
      121 gccggccgat aggcgatcga ctggctgcgg cggaagccgc catgggtcag gagatcgttg
      181 aggtcgcccg ccttgtcgcc gaccagatgc gtgaacacct tgcgctcgtg ccggcccggc
      241 aaataagggc agggcgaagg cgccgtgagg taaaattggg gggtgtcacg cgaatgctgg
      301 gtcaaggaat ttcgtcaagc ctccgggcca agcgggccgt tagcatcgcg ctgcggaggc
      361 tgacggtcaa ttgggtaagt tgcggtcagt aggtccttgc cgattgtgcc acgtgggtcc
      421 tcactgaatt gttgataatt acggttccca gcacaaaatc atgcaacagg cgtcgccgag
      481 cgttgaatgg cccaatcaga agcacaaagg gcgacagtac cgagatcgaa atccagaaaa
      541 gcacagcgtg catcgcaccc aagagaaaat agccgggcgc gccgtaccag gtgcgcagct
      601 ccaggtccat catccgcata ccaatggttg ccgaatgcga cccgccgata gaggcgccgt
      661 agtagatcag cgcccagatg atcgaggccg gcgaaaccag ccagaacagg gcccagccga
      721 ggccgagcgt cacaaggcca aacaccgcaa tgaacaggat cgccagcgcg atcgggatcg
      781 acagcacgat catgtcgatg atgaaggcga tcatccggcg catcgccaca ccgcgaaaca
      841 ggtccggctc cacgtaagga tcgaacgcat gctggtaagt ggggtcgcca gcgccctgcc
      901 agccgtcgcc cgtattgccc gaaccggtat atgacatgct cattcctcca gaacctgcgc
      961 tttaagcgga ttcccggcac agcacatggg aaccgcccgg gcgcttgcaa ggcctccggc
     1021 ccattacaga agcgcaatct tgcgtggatc aggccgttcg gccctgcgcc gccaattttt
     1081 cagccgccag cggcgcgaaa taagtgagca cgccatcggc accggcgcgt ttgaagccaa
     1141 gcaggctttc cattatcgcg cgttcgccgt cgatccagcc attattggcg gcgcccgcga
     1201 tcatggcgta ttcgccggag acctgatagg cgaaggtcgg cattccaaag gtgtccttca
     1261 cgcgcctgac gatgtcgaga tagggcatgc ccggcttcac catcaccatg tcggcgcctt
     1321 cggcgatgtc gagttcgacc tcgcgcagcg cctcgtccga attgccgctg tccatctgat
     1381 aggtgcgctt gtcgccggtg agcgtcttcg ccgagccgat cgcatcgcga aatggtccgt
     1441 agaaggcgga cgcatatttg gcggcatagg ccatgatctg cacgtccaga aatcctgcac
     1501 gatcgagcgc ctcgcggatc gcaccgacgc ggccgtccat catgtccgac ggcgcgatga
     1561 tgtcgcagcc ggcgtccgcc tggaccagcg cctggcgcac gagcacggca acggtctcgt
     1621 cgttcaaaat cctgccgtct tcgagcaggc cgtcatgccc atggctggtg aacggatcga
     1681 gtgcgacatc gcagaggatt ccgagatcgg gaaactcttt cttgattgcg cggacggact
     1741 ggcagaccag attgtcggga ttgacggctt ccgaaccctg ctcgtcgcgc agggattgat
     1801 cggtataggg aaagagtgcg atgcagggaa tgttgagctt catcgcgcgc tcggcctcgc
     1861 gcacggcctg gtcgaccgag agccgatcga cacccggcat cgacgcgatc gccgtgcgct
     1921 tgttgtggcc gtcggccacg aacaacggcc agatcaggtc gtcggaggtc agcacgttct
     1981 cgcgcaccag gcggcgcgcc cactcggcct tgcggtttcg gcgcgggcga acggtcagat
     2041 cgagggcagg ggcggcgctg gtcgcgctgt cccgccgcgg cccgtcgcgc atttcgatcg
     2101 gacgcccgaa tttgatcgcc atgagcggta tctcctgaaa ctgctgctgg gttggaattt
     2161 ttcgagtttt agcactgaaa accgtctcag tcacttgagc ttgcgcaagg ctgacgcgct
     2221 tggcattgat tttgccggat aaagcagcca agaatcgccg caaatgacac ttccaaaagc
     2281 tccactttcg aacgaaagca gccagccgca tgtctgacac ctctccgcgc gaggcggcgc
     2341 gagatgccat gtcggcggcg ctgtcctccg aacgaataga cggcaacgag aatgtctgga
     2401 cccggcgcct cgtgctgttc ctgcgcatca tggcagtgct ctcgatcctt aaagggctgt
     2461 accactgggc gcaggtcacg ggttttgtcg gcggcgagga agaggcgttc gagaaccagc
     2521 cgatggcctg gcaggctgcg accgtgtatt tcgcggtcat cgagcttgtc gcggctgtcg
     2581 gcctgtggct cgcaacgccc tggggcgcag tggtgtggct caccaccgtc gtgtcgatgg
     2641 cagtcatcga gctgatgttc ccggggatct atggcggcag ccttctggtg gtggcgggcg
     2701 aagccctgat gctgaccgct tatctcgcac ttgcctggat ggcggcgcgc gagcgcccgc
     2761 cgtagcgagc tgctctcgca taaaattttc agtgaatttc gattaaggtt tggggcacct
     2821 taagagcccc gccatacagg ttacgccgac cttgccgatt gcggcctggc tgttttggaa
     2881 tgcgacaaca agccaagacg aagcgtgaac aggggccttc caccgtcaac aacgggttgc
     2941 aagaaaccct ttatccatgg gcttaatccg cgatttactg tcttaaattc attctctctt
     3001 tattgtctta tttaagctga tcttcaaacc acccccttaa gttgcccctc acagacggga
     3061 ccaaagtttc gtcgaataag tcgttaaaac gacaacaggg gaagtgtcat gatgaaagcc
     3121 gttgcctcgg cggtggaaac cgctgaacgt gcgcctggcc agtcgcaagt gcaggcgctc
     3181 tatctggaag ctttgacgct ggtggaacgg ctgcatcgcc gtttgctcga cgtcatcaag
     3241 gacgaattcg atcgtcgcgg ccgttccgac atcaattcgg tacaggcgct gttgctctac
     3301 aatatcggcg acaaggagct gaccgcgggc gagctgcgca cgcgcggtta ctatctcggc
     3361 tccaacgtct cctacaatct gaagaagctg gttgagctcg gtttcctcga tcatcagcgc
     3421 tcgcgcgttg atcgccgctc ggttcgcatt cgcctgaccc cgcagggcca ggagatccgc
     3481 cgcgtcgtcg aggcgctgta tcagaagcac gtcaagacgg tcgagcaggt cggcggtatc
     3541 tcgaacgagg agttcgcgac cctgaacaag tcgctgcatc ggctcgagcg gttctggacc
     3601 gatcagatcc tctatcgtct ctgagagttt ttcggccaag ccggcgactg aagaagaccg
     3661 gcgggccgac aaaagcttcc ccttttggcg catcggccgg tcgtaaccgg gccgatgcgc
     3721 ctttttttgt ttcatgagtg tagcggcgcg gaaccttttc cctcctcgag acttatcgca
     3781 cgccgaacgg agggacttca ggtcatgctg gtcgaacgtg gagttcgcgt cctgaacacg
     3841 gaggtggtgg gtgatgccta cgccatcgcc tcgaattatc tgcgtcgcgc cggcgcaatc
     3901 tccgcaaatg tcgcgcccaa cgagcgcctg ctcggaatca tcgtgcggct gttcaaagac
     3961 ggcgaaatca acaaaatcag gctcgccaat caggcgattg ccgaattcga agccgccgaa
     4021 accaattgaa cgacatgtcg gagcagtcaa tggaagccgc aatcgatcgc atcatgcaga
     4081 catacgatct cctgatgaac cgcacgccgg aagcgaccgc ggtggcgcgc gaaaaggtga
     4141 cgcagtacgt caacacgctg ttcgaggccg gcgagcgcga tccgcatcgg ctcgccgtct
     4201 gcgggctcgc ctacctgcgc gaactcgacg gcctcaccga tccggtcaag gccggatata
     4261 ccgggctctg acgcgagtac ggctggtttg cgcgcgcccc aaaggcgctc cctgtcctgc
     4321 ttgcgcactc tccaggggag gacgaacgaa aatcaaatcg gcttgcctgt gtacggcatc
     4381 gacgcagtga ggccgccatc gaccggaaat gcctgaccgt tgacgtagga cgcttcgtcg
     4441 ctcgccagga acaatcccat cgccgcaatc tcctggggtt ggcccgggcg cttcagcgga
     4501 ttgagctgac cgatcttgtc ctgcgttccg cgatccttcg cacggtcgaa gatcggcttg
     4561 gtcatcccgg tctcgatcag gccgggacaa actgcgttga tgcgcacgcc cgtgccggag
     4621 agcgaatagg ccgtgatctg caccagactg atcacgcctg ccttgctcgc accataggga
     4681 tgtccgcttg cgccggattt cagtcccgca acggaagcgg tgcagacgat cgagccggaa
     4741 ccctgtttgg tcatgtgcgg aatggaatgc ttgatcgcga ggaacgggcc gatcagattg
     4801 atgcggagca cctcctgcca gtgctcgacc gtctgctctg cgagcgggac gagcccgccg
     4861 gagacaccgg cattggccca gatggcgtcg agcttgccgt aagcggagag agccttgtcg
     4921 atgaaggcaa tgacgtcttt ctcggaaccc gcgtctgcct gcaccgcttc ggccgtgcct
     4981 ccggccttgc gcactatctc gacgatttcc ttcacgcctt cggcgcggtc gaccgcgatc
     5041 agtttagcgc cttccctggt gaacagcagc gatgccgcgc gcccgatgcc gctgcccgcg
     5101 ccggtgatga tgacggattt gccttcgagg cggcccattt gttttctccc tggcggaata
     5161 tcaaacaaga tttgaaacgc cgaagtctct tgagacggca ttgggtctca cgtacagcga
     5221 caa
//
LOCUS       DATLJT010000248         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_931292,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000248 DATLJT010000000
VERSION     DATLJT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931292"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1682)
                     /locus_tag="VM910_05670"
     CDS             complement(285..1682)
                     /locus_tag="VM910_05670"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI62068.1"
                     /translation="MLPEPAVLDDGASRRRRPRPSRKPDYDHLPQIRVEHDVPDAEKV
                     CSHCGEPKARIGEDQARVLEFIPAHFELHVHVLPKYACSRCRDGVTAPETPPRPSSGC
                     IAGAGLLSGVIVSKFSDHLPLYRLEDISTREGLYLPRSTLCDWVGKVADLLRPLYELE
                     KKLVLDGPVLWTDDTSVTVLGGDKGGSHKGHFWVYIGGAALPYDVYDFTKNRQRDGPA
                     QFLVNYAGYLQADAFSGYDGIYTGSAGQIIEVACWAHARRKFFEARSSHPPEASLILQ
                     MIKRLYDVEDRARPLDDEARRALRQAEAVPVLERLELELNRLSIELLPKSALAQAVTY
                     ALNQWRALCRYPEDGRLTIDNNVSERRLRDQAIGRKNWLFLGNDEAGPRAAVLYTILA
                     GAKRHRLESWAYLRDVILQSSVDPSPEFLKPLLPDRWALAHPEHVLSHRVEESREKAR
                     RRDDRRARRRLPSKP"
     gene            1692..1940
                     /locus_tag="VM910_05675"
     CDS             1692..1940
                     /locus_tag="VM910_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62069.1"
                     /translation="MALVEFKEVPLSGSLDQAFTPRAKDIAAQKINLSVQLVNGLIVF
                     CDGLVVELRRLIQRGSKVFHLLAEPVQQLVALVRIGRP"
     gene            complement(1985..2374)
                     /gene="tnpB"
                     /locus_tag="VM910_05680"
     CDS             complement(1985..2374)
                     /gene="tnpB"
                     /locus_tag="VM910_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HVI62070.1"
                     /translation="MISVSHPVRVFLHTRPTDLRKGFDALSGLVITAFAQDPTSGHLF
                     LFVNRRRDRLEILYWDRDGLAIWYKRLETPGSFQLPAVSQDAVSVEMSHTQLTLMLSG
                     IDLRSARQRKRYHRPALEAEKVSRAPL"
BASE COUNT          586 a          950 c          982 g          546 t
ORIGIN      
        1 ggaatcggcg ccgagactcc ggtgagaagc ggcccgccga tgcgcccgaa gcgttctgcc
       61 cagacggccc gcccccggcc ggcgcggccg ccggccgcct tgccggggaa gagcaggact
      121 ggcacgtcgt gcccgatacg agtgcggaag gggcgtcgcg ttcgacgctg gcggcgtaag
      181 cgttcgggac taccagtatg acgcgcaccc ggccactgtc gcgtcacgac gcggtgctca
      241 cggcgcgctg gcactgagtc gcggcgccga tcgcaccaac ggcactatgg cttcgatgga
      301 agtcggcgcc gtgcgcgacg atcgtcgcgg cgccgcgctt tctcgcgcga ctcttcgacg
      361 cggtggctca atacatgctc agggtgcgcc aaagcccagc gatccgggag cagaggcttc
      421 aggaactccg gactcggatc gaccgagctt tgaaggatca cgtcacgcaa gtaggcccaa
      481 gactcgagcc gatgccgctt cgcacccgcc aggatcgtgt atagcaccgc cgcacgcggg
      541 cccgcctcat cgttgcccag gaacaaccag ttcttgcggc cgatagcctg gtcgcgaagg
      601 cgacgttctg acacgttgtt gtcgatcgtg agccgcccgt cctccgggta ccggcataac
      661 gcccgccatt gattcaacgc atatgtcacc gcttgcgcca gcgccgactt gggcaacagc
      721 tcgatcgaga ggcggttcaa ttccaactcg agccgttcca gaaccggcac cgcctcggct
      781 tggcgcagcg cgcgccgcgc ttcgtcgtcc aggggtcgag cccgatcctc gacgtcataa
      841 aggcgcttga tcatctgcag aatgagtgat gcttccggcg ggtgcgacga tcgcgcctcg
      901 aaaaacttcc ggcgcgcgtg cgcccagcag gccacctcga tgatctggcc ggcagagccc
      961 gtatagattc cgtcgtaccc actgaacgca tcggcctgaa gataccctgc gtagttcacc
     1021 aggaactgcg caggaccgtc gcgctgacga ttcttcgtga agtcgtagac atcgtagggc
     1081 aacgcagcgc cgccaatgta cacccagaag tgccccttgt ggctgccgcc tttgtcgccc
     1141 cccaacacgg tcacagaggt atcatcggtc cagagcaccg gcccgtcgag cacgagtttc
     1201 ttctccagct cgtacagggg cctgagcaag tcagcaacct tgcccaccca atcgcaaagg
     1261 gtgctgcgag gcaaatagag cccctcgcga gtcgagatat cctcgagccg atacagcggc
     1321 agatggtccg aaaacttgga gacgatcact cccgacaaca agccggcccc cgcgatacaa
     1381 ccgctcgagg gacgcggagg agtctcggga gcggtaaccc cgtcgcggca gcgcgagcag
     1441 gcgtacttgg gcaggacgtg aacgtgtaat tcaaagtgtg ccggaatgaa ttccaggacg
     1501 cgtgcctggt cttcaccgat acgtgccttc ggctctccac agtgcgagca taccttctcg
     1561 gcgtcaggca catcgtgctc gacacggatc tgagggagat gatcgtaatc gggcttgcgt
     1621 gatgggcgcg ggcgcctgcg tcgtgacgcc ccgtcgtcga ggacagcagg ttccgggagc
     1681 acggcggcgc cttggccctg gtcgagttca aagaggtgcc cttgtccggg agcctcgatc
     1741 aggcgttcac gccgcgggcc aaagatatag cggcgcagaa gatcaatttg tcggtgcagc
     1801 tcgtcaacgg cttgattgtg ttctgcgacg gtttggtcgt ggagcttcgc cgcttgatcc
     1861 agcgcggttc gaaggtcttc cacctgctgg ctgagccggt tcagcagctc gtggcacttg
     1921 tcaggatcgg tcggccatga cgcggggtta ctcatgcccg gaattatagc gcgatcattc
     1981 gctgttacaa gggggcacgg ctcacttttt cagcctccag cgccggccga tggtaccgct
     2041 tgcgctgccg agcggagcgc aggtcgatgc ctgagagcat gagcgtcaat tgggtgtgag
     2101 acatctcgac ggatacagcg tcctgggaaa cggcggggag ctggaacgaa cccggcgttt
     2161 cgagtcgttt gtaccagatc gcgaggccgt cgcgatccca gtacaggatc tcgagccggt
     2221 cacggcgtcg attgacgaac aggaacaagt ggccggaggt cggatcctga gcaaaggctg
     2281 tgattaccag gcccgagagc gcatcgaagc ctttacgaag gtctgtcggt cgggtgtgga
     2341 gaaagacccg gaccggatga gagacgctga tcattgcacg tctcccaatc gagttcgagc
     2401 tcgtgctaca gcgcggacaa cacggcagac caggcgagcg tctgacgtcg gaatccgcag
     2461 gcggacccca ttgggaagat cggcttcgat gggcgcttca ccggcagctt cctcaaccac
     2521 gcgcacggtg accggcacga acgcggctgg ggcaggcaat gcgggccgag ccggaagtac
     2581 gagattgacg agtcgaaagc ggcgcttcca attcgagaac gagcccacgg agatacgttc
     2641 ttgcgcgcag aactgggcga tcgtgagccc acttcgggcc tgacgctcga cgcgctggcg
     2701 ccaagcggaa tgaagctcgg gatcgggctt tcgcggcatc gtgcaggctc ctgtgaaggg
     2761 atgagttatc cgctccacag gctaccaggg cctgcaacga tggttatctc ggacgcttac
     2821 gcttcgcgag catgaagaga agcaccgcac atagggtgcg ctattgcgga cctcttctct
     2881 ccgcgcgctg gctcgaggtg cgcaacagac gcgtacggcc ccctattgcg gaccgcttct
     2941 gtcggtgggc gggctcatgg tgcgctacac gctggagtcc caaatcctta ggacatcata
     3001 tcgacatcaa ttcctagaat ttgattgccc ggcctttcgg cgcgcgctat tgtgctgaag
     3061 cacc
//
LOCUS       DATLJT010000249         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1850425,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000249 DATLJT010000000
VERSION     DATLJT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850425"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VM910_05685"
     CDS             complement(<1..375)
                     /locus_tag="VM910_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HVI62071.1"
                     /translation="MIRAIDLFSFSLPLLRWLDAEDAHRLAVQGLKFLPAMRPHADDP
                     KLAVRAFGLNFPNPIGMAAGFDKNAEVPDALLRLGFGFVEVGTVTPRPQSGNPRPRLF
                     RLERDEAVINRMGFNNDGSDRVL"
     gene            complement(372..716)
                     /locus_tag="VM910_05690"
     CDS             complement(372..716)
                     /locus_tag="VM910_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HVI62072.1"
                     /translation="MHTIYKICSASAWREAERQGVYHGSADDARDGFIHFSTATQVAE
                     TARKHFFGQTGLFLVEVDADKLGQALRWERSRNDQLFPHLYGELDLGAVLRVMTMHTR
                     SDGTHDIPELKP"
     gene            865..1518
                     /locus_tag="VM910_05695"
     CDS             865..1518
                     /locus_tag="VM910_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI62073.1"
                     /translation="MPRQPRPQKTLTHAQIWGALDQLAARAGLSPSGLAKRSGLDPTT
                     FNKSKRITAQGRERWPSTESVAKALLAAEQSIENFVDLIGDGAPPERSVPLIALAQAA
                     HRMHFDDRGRPSGKGWEEVALPSLEDDQSFALEISGDALKPAYRDGDVVVVSQRAPIH
                     RGDRVVVKTRDGEVMIKELKRRNARTLEFASVNSAHADRTLAVADIDWIARIVWVRQ"
     gene            complement(1540..>2062)
                     /locus_tag="VM910_05700"
     CDS             complement(1540..>2062)
                     /locus_tag="VM910_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI62074.1"
                     /translation="GDVERSCYCIHAPIHKALPQAKAVFHTHMPFASALTRLEDPCIK
                     EIGQTEVGMINEIAYDDEYTGPALDPEEGARLARVIGEKSILFMANHGISTVGVTVAE
                     AYDRLYYVERAAQVQIYAMWTGQKLKQLPAPVVAKTKRDIGGGSRYDPPPAQRHFDAL
                     KRLLDREEPDYAT"
BASE COUNT          383 a          644 c          669 g          366 t
ORIGIN      
        1 cagcacgcgg tcggagccgt cattgttgaa gcccatgcgg ttgatcaccg cctcatcgcg
       61 ctcgagccga aacagccggg gccgagggtt gccgctctgc gggcgcggcg tcaccgtgcc
      121 gacctcgaca aagccgaaac caagccgcag cagcgcatcc ggcacctcgg cattcttgtc
      181 gaaacctgcg gccatgccga tcggattggg aaaattcagg ccgaaagcgc gcaccgcaag
      241 cttggggtcg tcggcgtgcg gccgcatggc cggcaggaat ttcaggccct ggaccgcaag
      301 acgatgcgcg tcctcggcat ccagccagcg caacagcggc agcgagaacg aaaagaggtc
      361 gatggcgcgg atcacggctt cagctccggg atgtcgtgag tgccgtcgga gcgggtgtgc
      421 atcgtcatca cgcgaaggac cgccccgaga tcgagttcgc cgtaaagatg cgggaacaat
      481 tggtcgttgc gcgagcgttc ccagcgtagc gcctggccaa gcttgtcggc atcgacttcg
      541 accagaaaca gcccggtctg gccgaaaaaa tgcttgcgcg cggtctcggc aacctgagtg
      601 gcggtcgaga aatgaataaa cccgtcccgc gcatcgtcgg cgctgccgtg gtagacgccc
      661 tgccgttcgg cctcccgcca ggcggaggca gagcagatct tgtagatcgt atgcactgtt
      721 tgaattgatt tggcttggag tttttcgaaa accaaggctc gaccgtatcg accgcttcga
      781 tcgaactcaa gggcgcgttc cgcccttgcg gaggacggcg aaaggcgttt tattgaggta
      841 attattccta ttggctccgc tctcatgccc aggcaaccaa ggccgcagaa gaccctgacg
      901 cacgcgcaga tctggggcgc gctcgatcag cttgcggcac gcgccggact gtcgccgtcc
      961 ggccttgcca aacgcagcgg cctcgacccg accacgttca acaaatccaa gcgcatcacc
     1021 gcgcagggtc gcgaacgctg gccgtcgacc gaatccgttg ccaaggcgct tctcgcggcc
     1081 gagcaatcga tcgaaaattt tgtcgatctg atcggtgacg gcgcgccgcc cgagcgctcg
     1141 gtgccgctga ttgcgctggc gcaagccgcg caccgcatgc actttgacga tcgtggccgt
     1201 ccgtccggca agggttggga ggaggtggcg ctgccatcgc tcgaggacga tcagagtttc
     1261 gcattggaaa tttcgggcga cgcgctgaaa cccgcctatc gcgatggcga cgtcgtcgtg
     1321 gtgtcgcaga gagcgccgat ccaccgcggc gaccgcgtgg tggtgaagac ccgcgacggc
     1381 gaggtgatga tcaaggaatt gaagcggcgc aatgcaagga cgctggagtt cgcctcggtc
     1441 aattccgccc acgccgatcg cacgcttgcg gtcgccgaca tcgactggat cgcgcggatc
     1501 gtgtgggtga ggcagtaggc tcaacgccga gcaaagcgac tacgtcgcgt agtccggctc
     1561 ctcgcggtcg agcagacgct tcaaagcatc gaaatggcgc tgcgccggcg gcggatcgta
     1621 gcggctgccg ccgccgatat cgcgcttggt ctttgcgacg acgggcgcag gcagttgctt
     1681 cagcttctgt cccgtccaca tcgcatagat ctgcacctgc gcggcgcgct cgacataata
     1741 gagccggtca taggcctcgg cgacggtcac cccgacggtc gagatgccgt gattggccat
     1801 gaacaggatg ctcttctcgc cgatcacgcg cgcaagccgc gcgccttcct ccgggtcgag
     1861 cgccggcccg gtatattcgt cgtcataggc gatctcgttg atcatgccga cttcggtctg
     1921 accgatctcc ttgatgcagg gatcctccag ccgcgtcagc gcgctcgcaa agggcatgtg
     1981 ggtatggaac acggccttgg cctgcggcag cgctttgtgg atcggcgcgt gaatgcaata
     2041 gcaggagcgc tcgacgtcgc cg
//
LOCUS       DATLJT010000250         2619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2999294,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000250 DATLJT010000000
VERSION     DATLJT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999294"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VM910_05705"
     CDS             complement(<1..1012)
                     /locus_tag="VM910_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI62075.1"
                     /translation="MKTSTRRGFLNAAATAISGVIAGGAAPPSLANAAPYDADAIASE
                     ISAKLFADDGLARPIPKTPTVSDLAKGLDRTLVLGGGGEYYVAWYCGFFHGLHELGVD
                     LNIAEMVVGTSAGSYAGSSLTSGHFQRLRLEFDFFGHFPTLFARLAPVASPNLSQKRA
                     QEINFSVKDGSPASIRAIGHAALAADNRVNGSAVERLAGLLTGDSTTGWPVAKMYTTA
                     NDCYTGERLIVGQEVARRNGIPLAHAAAASSSLPGVIGPTLLGQRFCMDGGISRTWAH
                     TDVVAGSKRALVITLTNGYEGSLLSGIPHNIYKEIDALQATGTQTMLIIAGTPAGVNL
                     LDP"
     gene            complement(1323..2318)
                     /locus_tag="VM910_05710"
     CDS             complement(1323..2318)
                     /locus_tag="VM910_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62076.1"
                     /translation="MASYIGRRELLAALGGAAAAWPFAARAQQPAMPMIGFLNARSPE
                     DTIHLVAAFRRGLGEGGFIEAQNVTIEYRWALGQYDRLPAMAAELAHRPVTLLATAGG
                     EPAALAAKSATSTIPIVFVIGGDPVKQGLATSFNLPGGNSTGVSILTTTLEPKRLGLL
                     RELVPGTAMIGVLGNPSLSEYESQLRDVQEAAGTLGLQILVLPVNTDHDVEAAFEAVV
                     QRRIAALTVLAAPFLDTRRDKLVALAARHAVPTMYQFREFVAAGGLVSYGVNVADAYQ
                     QVGLYAARILKGEKPADLPVVQPTKFELLINLKTARALGLTVPDRLLALADEVIE"
BASE COUNT          510 a          798 c          821 g          490 t
ORIGIN      
        1 cgggatcgag caggttcacc ccagcgggcg ttccggcgat gatcagcatg gtctgggtac
       61 ctgtcgcctg gagagcgtcg atctctttgt agatgttgtg cgggatgccg ctcaacaggc
      121 tgccctcgta cccgttggtc agggtgatga cgagtgctcg cttggagccg gcgactacat
      181 cggtgtgcgc ccaggttctg gagatgccgc catccatgca gaaccgctgg ccgagcaatg
      241 tcggaccgat cacgccgggc agcgatgaac tggcggcggc cgcgtgggcc aacggaatgc
      301 cgttcctgcg cgctacctct tgccctacga tcagccgctc gcccgtgtag cagtcgttgg
      361 cggtggtgta catctttgcc accggccagc cggttgtgct gtcgccggtc aacaggcccg
      421 ccagccgctc gactgcgctc ccgttcaccc ggttgtcggc ggcaagcgcc gcgtggccga
      481 tcgcgcggat tgaggctggg ctgccgtcct tgacgctgaa gttgatctcc tgcgcccgct
      541 tctggctgag gtttgggctg gcgacaggcg cgagccgcgc gaacagcgtc gggaaatggc
      601 cgaagaaatc gaactccagc cgcaagcgct ggaaatgccc agaggtcagt gacgagcccg
      661 cgtaggaacc ggccgaggtt ccgacgacca tctcggcgat gttgagatcg acgcccagct
      721 cgtgaaggcc atggaagaac ccgcaatacc aagcgacgta atattccccg ccgccgccaa
      781 gaaccaaggt gcggtcgagg cccttagcga ggtcggagac agtgggcgtc ttggggatcg
      841 gtcgggccag tccgtcgtcg gcaaaaagct tagccgagat ctcggacgca atcgcgtccg
      901 cgtcgtaggg ggcggcattc gccagagatg gcggcgccgc gccgccggcg atcacacccg
      961 agatggcggt cgccgccgcg ttgagaaagc cgcgtcgagt ggatgtcttc atccgttact
     1021 ccgcagatac gccacctgtt tgaccgatcc gtggaacgcc atgatcgtcg gccaagataa
     1081 tcactgcagg cgcgtggtca tgtccaatac gatacggctc ggacggtagc acgcatgctc
     1141 cctcagaaga cggtgcactg cgggaaattg accccgccta tgtccgctat gggtcataag
     1201 cggaaaaact cgatctgagc attagatgtc cgctttaccc ccaaaagcgg acaaacagga
     1261 gaaggctcga cttgtccgct ttgcgccaaa agcggacata tgcagtgcag caaaagagat
     1321 ggctattcga tcacctcgtc ggcgagcgca agcagcctgt ccggcaccgt gagcccgagc
     1381 gccctggcgg tcttgaggtt gatcaggagc tcgaatttgg tgggctgtac aaccggcagg
     1441 tcagccggtt tctcgccttt gagaatgcgc gcggcataga gcccaacttg ctgataggcg
     1501 tcagcaacgt taacgccgta gctcaccaga ccgccggccg caacgaattc acgaaactga
     1561 tacatcgtgg gcactgcgtg gcgcgctgcc agcgccacaa gcttatcgcg tcgcgtgtca
     1621 aggaacggag cggccagcac cgtgagtgcg gcaatgcgtc gttgaacgac agcttcgaat
     1681 gccgcctcaa cgtcgtgatc cgtgttaacg ggcagcacaa ggatctgcaa acctagggtg
     1741 ccagcggcct cctgcacgtc tcgcaactgg ctctcgtact cggaaagact cgggttcccg
     1801 agaacgccga tcattgccgt ccctggcacc agctcacgca acagcccgag ccgtttgggc
     1861 tccagcgtgg tagtcaagat acttaccccg gtagagtttc cgcccggcag attgaagctc
     1921 gttgcgagtc cctgcttgac cggatcgcca ccaatgacga acacaatcgg aatggtcgaa
     1981 gttgcagact ttgcggccag cgcggcaggc tcaccgcccg ctgttgctaa gagcgtcaca
     2041 gggcgatgag ctaactccgc tgccatcgct ggcagccggt cgtattggcc gagtgcccag
     2101 cggtattcga tggtgacgtt ctgagcttcg ataaagccgc cttcccccag gcctcggcgg
     2161 aacgcagcca ctaggtggat cgtatcttcg ggggacctgg cgttgagaaa cccgatcatc
     2221 ggcatggccg gctgctgcgc ccgcgccgcg aacggccacg ccgccgccgc gccgccgagt
     2281 gcagccagca attcccgccg tccgatgtag cttgccatgt gaccctgcgg ttgggggtca
     2341 ttcatgcaat ggagcgatga taccacgctt tgagcgtgcg gtcagtgact acttcagagc
     2401 gccgtcaacg ttgactgcaa cggtaaaagt ctgaaagccg gtcgcgatta cccgtgccgc
     2461 aaaatcagtt tttgaacggt agcaggtttt caccgttcca atttcgcggt gttcgagcgt
     2521 aaagaagtcg tcacgcgacg cgcgaggtga tagaacagtc accgcgagag gccttgtcgt
     2581 ttacgttatc gtaagctcgc gctgctcctg cgacgcagg
//
LOCUS       DATLJT010000251         2680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4148549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000251 DATLJT010000000
VERSION     DATLJT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4148549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VM910_05715"
     CDS             complement(<1..1217)
                     /locus_tag="VM910_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HVI62077.1"
                     /translation="MHIVDRRLNPGGKSLENRQRFLRRAKSLVQAAVKKSSQDRDIKD
                     VLEGGEVSIPLDGMHEPRFRREGGTRDMVLPGNKKFVEGDMLPRSGGGGGGRPTEPGE
                     GDSEDAFRFVLNRDEFVDLFLDDLELPELTKRRLADVESEGLHRAGYTTTGSPANLSI
                     SRTVSNALMRRVALQRPRPQVIAELEAELEDCDEARRAELLAKIATLKSKIRRIPFID
                     PIDIRYRRFENVPKPVAQAVMFCLMDVSGSMSEHMKDLAKRFYMLLYVFLKRRYRHVE
                     IVFIRHTDRAEEVDEETFFHGPASGGTLVSSALQAMHEIIRSRFRPADWNIYAAQASD
                     GDNLSADGEVAGRILTEHILPVSQFFAYLEVGEAGGYSFEMPDSSLWTLYERLRSAGA
                     PLSMRKVHDRSEI"
     gene            complement(1237..>2680)
                     /locus_tag="VM910_05720"
     CDS             complement(1237..>2680)
                     /locus_tag="VM910_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HVI62078.1"
                     /translation="YGIPRRRLTGLMSPWCYKRLESFGGDISQFRVARIQPSRLRQIA
                     ITKTEPGDENNQDISSLVGKVDIRKLETFAQNDPDAYSYSGGLNRANQGILEFVEMFK
                     APIKMLHPLLTATQEGNYIGTENIGAIPFSGIILAHSNEAEWQSFKSNKNNEAFIDRI
                     CVIKVPYCLRVTEEQKIYEKLIQGSELAAAPCAPATLETLARFSVMSRLRKHENSTLF
                     AKMRVYDGESLKESDPKARSVQEYKDAAGVDEGMDGASTRFAFKVLAATFNHDTTEIG
                     ADAVHLMYTLEQAIRREQLSDEVEKRYLEFIKADLAPRYAEFIGNEIQKAYLESYADY
                     GQNLFDRYVDYADAWIEDQDFKDPDTGQLMDRELLNQELTKIEKPAGIANPKDFRNEV
                     VKFSLRSRAQNGGKNPSWTSYEKIREVIEKRIFSQVEDLLPVISFGSKKDGETEKKHG
                     EFVNRMVARGYTERQVRRLVEWYMRVKQAG"
BASE COUNT          474 a          837 c          811 g          558 t
ORIGIN      
        1 aagatctcgc tgcggtcgtg caccttgcgc atcgacagcg gcgcgccggc cgagcgcagc
       61 cgctcgtaca gcgtccacag cgaggagtcc ggcatttcaa acgagtagcc gccggcctcg
      121 ccaacctcca gataggcgaa aaactggctg acggggagga tgtgctcggt cagtatccgc
      181 cccgctacct cgccatcggc gctcaggttg tcgccgtcgg aggcttgcgc ggcgtaaatg
      241 ttccagtcgg ccgggcggaa acgcgagcgg atgatctcat gcattgcctg caacgcgctc
      301 gacaccagcg tgccgccgga agcaggccca tgaaagaacg tctcctcgtc gacttcttcg
      361 gcgcggtcgg tgtggcgtat gaagacgatc tccacgtgcc ggtagcgccg tttcagaaaa
      421 acgtagagca gcatatagaa gcgcttggcg agatccttca tgtgctcgga catggaccct
      481 gagacgtcca tcaggcagaa catgaccgct tgcgcgactg gcttcggcac gttttcgaag
      541 cgccgatagc ggatgtcgat gggatcgatg aagggaatcc ggcggatttt cgatttcagc
      601 gtcgcgattt tcgccagcag ctcggcgcgc cgggcctcgt cgcaatcctc cagttcggct
      661 tcgagttccg cgatcacttg cggccgcggc cgctgcaacg cgacgcgacg catcaaggca
      721 ttgctcaccg tgcggctgat ggagaggttt gccggcgaac ccgtcgtcgt atagccagcg
      781 cgatgaagtc cctcgctttc gacgtcggca agcctgcgct tggtgagctc cggcaattcc
      841 agatcgtcga ggaaaaggtc gacgaactcg tcgcggttca ggacaaagcg gaatgcatcc
      901 tcgctgtcgc cctcgcccgg ttcggtcggc ctgccgccgc cgccaccgcc cgaacgcggc
      961 aacatgtcgc cttcgacgaa tttcttgttg ccaggcagca ccatgtcgcg cgtgccgcct
     1021 tcgcggcgga agcgcggctc atgcatgccg tcgagcggta tggagacctc accgccctcc
     1081 agcacatcct tgatgtcgcg gtcctgcgag ctctttttga cggccgcctg aaccagcgac
     1141 ttggcgcgcc gcaagaaccg ttgccgattc tcgaggctct tgccgccggg attcaggcgc
     1201 cggtcgacga tatgaatggc cactgtcgca tccgcctcac ccggcttgct tcacccgcat
     1261 gtaccactcg accagccgcc ggacctgccg ctcggtgtag ccacgcgcga ccatgcggtt
     1321 cacgaactcg ccgtgcttct tctcggtctc gccgtctttc ttcgaaccga aggagatgac
     1381 cggaagcaaa tcctcgacct gcgagaatat ccgcttctcg atgacctcgc ggatcttctc
     1441 gtaactggtc cagctcgggt tcttgccgcc gttctgggcg cgcgaccgta gcgagaactt
     1501 gaccacctcg ttacgaaaat ccttcgggtt ggcgatgccc gccggctttt cgatcttggt
     1561 cagttcctga ttgagcaatt cccgatccat caactggccg gtgtcgggat ccttgaaatc
     1621 ctgatcctcg atccaggcgt cagcgtagtc gacgtagcgg tcgaacaggt tctggccgta
     1681 gtcggcatag gattcgagat aggccttctg gatttcgttg ccaatgaact ccgcgtagcg
     1741 cggcgcaagg tccgccttga tgaactcgag atagcgcttc tcgacctcgt cggacagctg
     1801 ttcgcggcgg atggcctgct ccagcgtata catgaggtga acggcgtcgg cgccgatctc
     1861 ggtggtgtca tgattgaagg tcgcggccag caccttgaac gcaaaccggg tcgaggcgcc
     1921 gtccatgcct tcgtcgacgc ctgcggcgtc cttgtactcc tgaacgctgc gcgccttcgg
     1981 gtcggactcc ttcaggctct cgccgtcata gacccgcatc ttcgcaaaca gggtcgagtt
     2041 ctcgtgcttg cgcagccgcg acatcaccga aaagcgcgcc agtgtctcca gtgtcgccgg
     2101 cgcacaaggt gctgcggcga gctcagagcc ctggatcagc ttctcgtaaa tcttctgctc
     2161 ctcggtgacc cgcaggcaat acggcacctt gatgacgcag atgcggtcga tgaaggcctc
     2221 attgttcttg ttggacttga agctctgcca ttcggcctcg ttggagtgcg ccaggatgat
     2281 gccgctgaac gggatagcac cgatgttttc ggtgccgatg tagttgcctt cctgggtggc
     2341 cgtcagcagc ggatgcagca tcttgatcgg cgccttgaac atctcgacga attcgagaat
     2401 gccctggttg gcgcggttga gcccgccgga gtagctgtaa gcatcaggat cgttctgggc
     2461 gaaagtttcc agcttgcgga tatcgacctt gccgaccagc gaggaaatat cctggttgtt
     2521 ctcgtcgccg ggctccgttt tggtgatcgc gatctgccgc aaccgcgacg gctgaatcct
     2581 ggctacgcgg aattgcgaga tgtcgccgcc aaaggattcg agacgcttgt agcaccaggg
     2641 gctcatcaac ccggtcagac gacggcgcgg aatgccgtac
//
LOCUS       DATLJT010000252         5218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4377879,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000252 DATLJT010000000
VERSION     DATLJT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377879"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VM910_05725"
     CDS             <1..242
                     /locus_tag="VM910_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62079.1"
                     /translation="TNFADGYRQAGIYAGRILKGEKPDDLPVVQATKVELVINLKTAK
                     ALVASGWRSWPMLIFLARCRKRLRFRWRPPSSTMR"
     gene            212..637
                     /locus_tag="VM910_05730"
     CDS             212..637
                     /locus_tag="VM910_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62080.1"
                     /translation="MAAAKLDHEVVVLEVRRAEDIAGAFESLKGRTDVLYVCGDLLTF
                     TNRTRINTFAQSARLPTIHGTREFVEAGGLVSYGVNYVHLYRRAAEHIDKILRGTNPA
                     DIPIEQPTKFDLVINLVTAKALGLTVPDTLLARADEVIE"
     gene            656..1636
                     /locus_tag="VM910_05735"
     CDS             656..1636
                     /locus_tag="VM910_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62081.1"
                     /translation="MKLPRRNFLHLMAGAAALPVVTRIAWAQAYPTRPVRIVVAAAAG
                     GPTDLLARLLGQWLTERLGQQFITENRPGGGGNIGTEAVVRAAADGHTLLLVNFVNAL
                     NASLYERLSYNFIRDIEPVASISRESNVMVVNPSVPSKTVPEFIAYAKANPGKINFAS
                     GGNGTPSHVTGELFKMMTGVNIHHVPYRGAAPALTDLLSGQVQIILAAPLSGAIGYVR
                     AGQIRALAVTNRTRSEMLPEVPTVGEFVPGFEASGFFGVGAPRNTPAEIVNKLNSEIN
                     AGLDDAKIKARLADVGNTVLPGSPADFGKFIVDETEKWAKVIKSAGIKPE"
     gene            complement(1716..2420)
                     /locus_tag="VM910_05740"
     CDS             complement(1716..2420)
                     /locus_tag="VM910_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62082.1"
                     /translation="MRLQRKSRSARRRLTPLVLCLGLAVSLSAAFAQDEPIPGAQSSR
                     LSVNKPAHFQIWRTITLGTYKDVDAYRDALDSAGVKIGLSADEILGRPAFPYAKIKID
                     VELAVLSAAELGVESDESSLSDVYERARQAGLELCPAEVGPKLRLNYRNQPLGEALDI
                     AMEPVATYSGDPTILTLANWGTGLMLIGRNGRPQSTVNRISRFVFALPMHGRLEAVSD
                     RQIVPTFSEQKAPAVR"
     gene            3126..3875
                     /locus_tag="VM910_05745"
     CDS             3126..3875
                     /locus_tag="VM910_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HVI62083.1"
                     /translation="MAVFDELRQGVQDIAFLTDPTVGEIRVGGTEGMISTVFSPVVHQ
                     LSTQYPRMSFRVVVGDLRTLYRELEARRVDVVVSRLHNPPSEEYSAEVLYEDTLVVVT
                     GLRNPLARRRKIEIAELLDEPWTFQPSDTNFGSFAMDAFRAVGLAPPRIAVATTSHTL
                     HNELLATGRYLAMVPRFWVLLQRPNPSFKVLPVAFPHTQHKVAIITLKNRSLSSATQF
                     FIDRVRAITKAVNEQKNRPMTKRGNPSKSPA"
     gene            complement(3841..4020)
                     /locus_tag="VM910_05750"
     CDS             complement(3841..4020)
                     /locus_tag="VM910_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62084.1"
                     /translation="MSVVGFMGTTSAAAWVEDMGLSEDLFVDPVPIVNGMITAPERPG
                     HGLAFKPEILKDCRV"
     gene            4039..4356
                     /locus_tag="VM910_05755"
     CDS             4039..4356
                     /locus_tag="VM910_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62085.1"
                     /translation="MVGDAVFALNRVRINTLALGARLPTMHSGREFVEAGGLMSYAPN
                     FPTLSRRAAEFVDKILRGAKPGEIPIEQPTKFDLIVNLITAKALSLEVSPTLLARADK
                     VIE"
     gene            complement(4738..>5218)
                     /locus_tag="VM910_05760"
     CDS             complement(4738..>5218)
                     /locus_tag="VM910_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI62086.1"
                     /translation="TEWVVYSKRPFGGPEAVLAYLSRYTHRVAIANSRLIAFDQDSIT
                     FKCKDYRIEGRDRYKRMTLATDEFIRRFLIHVLPKGFHRIRHYGLLAKTSGADNIARA
                     RELLAGPKPQSQPADATDHNQPACPCCGGRMIIIETFPRGATPRHQPTDPIIRIDTS"
BASE COUNT         1054 a         1519 c         1592 g         1053 t
ORIGIN      
        1 tcaccaactt tgccgacggg tatcgtcaag ccggcatcta tgccggccgc attctaaagg
       61 gcgagaagcc agatgacctg ccggtcgtgc aggcaactaa ggtcgagttg gtgatcaacc
      121 tcaagaccgc caaggcgctc gttgcctccg gctggcggtc atggccaatg ttgattttcc
      181 tggcgcggtg caggaaacgg ctgaggttca gatggcggcc gccaagctcg accatgaggt
      241 agtcgtcttg gaagtccgcc gtgcagagga catcgcagga gctttcgagt cactcaaggg
      301 gcggacagat gtcctatacg tctgcggtga tctgctcacc ttcaccaatc gaactcgtat
      361 caacactttt gcacagagcg ctcgcctgcc gacaattcac ggcacgcgtg agttcgtaga
      421 ggccggaggc cttgtatcct atggcgtcaa ctacgtgcat ctctaccggc gtgcggcgga
      481 gcatatcgac aagattctac gcgggacgaa cccggccgac attccaatcg agcagccgac
      541 caaattcgac ctcgtcatca atctggtgac cgcgaaggcg ctcggcctca ccgtgccaga
      601 cacgctgctc gcccgcgccg acgaggtgat cgagtgatgt gaagggaggt tcaacttgaa
      661 acttccccgc cgaaattttc tgcatctgat ggcgggcgct gccgcgctgc cggtagtcac
      721 gcgcatcgcc tgggcgcaag cctacccaac gcggccggtg cggatcgttg tggccgccgc
      781 cgctggaggg ccaaccgact tgctggcgcg tcttttgggt caatggctaa ccgagcggct
      841 cggccaacaa ttcatcaccg aaaatcgacc aggagggggc ggcaatattg ggactgaggc
      901 agttgtgcgt gctgctgccg acggacacac gctgctgctg gtcaatttcg tcaacgcgct
      961 caatgcgagc ttgtacgaaa ggctcagcta caatttcatc cgagacatcg agccggtcgc
     1021 aagcatcagc cgcgagtcca acgtcatggt ggtgaatccg tcggttccgt ccaagaccgt
     1081 tcccgaattc atcgcctacg ccaaggccaa tcccggcaag atcaacttcg catcgggcgg
     1141 caacgggaca ccaagccatg taaccggcga gctgttcaag atgatgaccg gcgtcaacat
     1201 ccatcatgtg ccatatcgtg gtgcggcacc cgctctgact gatctgctta gcggacaggt
     1261 gcaaatcata ctcgcagcgc cgttgtctgg agccatcgga tacgtcaggg ccggtcagat
     1321 acgtgcgctc gccgtgacca ataggacgcg ctcggaaatg ctgcccgagg tccccaccgt
     1381 gggcgagttt gtgcccggct tcgaagccag tggcttcttt ggagtgggtg caccgagaaa
     1441 cactcccgcc gaaatcgtta ataagctgaa cagcgaaatt aacgctggcc tcgacgacgc
     1501 caagatcaag gcgcggcttg ccgatgtcgg caatacagtg ctcccgggct cgcccgccga
     1561 ctttggaaag ttcatcgtcg acgaaaccga gaagtgggcc aaggtaatca agtccgccgg
     1621 catcaagccg gagtgatccg gaaattccgc gatattccat aacctcccgt tccgcgaatt
     1681 gaagtgtggg gcggcccttc aacggcgcgc ggggtttatc tgaccgccgg ggctttctgt
     1741 tctgaaaatg ttgggacgat ctgacgatcg ctcacagcct ccagacgtcc gtgcatcggc
     1801 agagcgaaca cgaagcgaga tatccggttc accgtagact gcggtcgccc attgcggcct
     1861 atgagcatga gaccggtgcc ccagttcgcc agcgtcaaga tcgtggggtc tccactgtac
     1921 gtggccacag gctccatcgc gatatcaaga gcttcgccga gcggctgatt gcgatagttc
     1981 aaccgcaact tcggcccgac ctcagcaggg cacagttcaa gaccagcttg ccttgcgcgt
     2041 tcgtacacat ctgaaagaga cgattcgtcc gattccacgc cgagttccgc ggctgaaagc
     2101 acggcgagct ccacatctat ttttattttt gcataaggga acgcgggccg accgaggatt
     2161 tcgtctgcag aaaggccaat tttaactccc gctgaatcca acgcatcgcg ataagcatca
     2221 acatccttgt aggtgccgag cgtgatcgtt cgccaaatct gaaaatgagc cggcttatta
     2281 acactcagcc tactcgactg cgcgcccggt attggctcgt cctgcgcgaa ggcggcgctt
     2341 agggagacgg caagtcctag acacagcacc agtggtgtga gccgccggcg agcgcttcgc
     2401 gatttacgct gcaatctcat tattggccac cccagcaatg gcaagcgtag ccggcttgga
     2461 tcgatgtaaa aaacgcgcca cagccgattt cgcgttgata ggcaatcttg caattggcga
     2521 tcgcgaaggc gattgctcgg ttgatcgaga agcaggtggc ggcgccccac aaaccatcgg
     2581 gcgccatcgt caacaccgtc caatcgtcgt cggcgacggc gggtgtcgcc actgacgacg
     2641 tggcgagtgt aacagcgaga gaggccacgc ttagagcgaa atgtaaacgg cgcatcgcga
     2701 tgctactccg tttcattgca ggcagcacgc tgcgatccgc atgggccgtc acctgtgacg
     2761 ttgcaccaca catcggtgtc gggggtccga tcgccgtgcg cgcctgcgaa ccagcaaaga
     2821 gcgtagcgga ccttgtcaca accagataat gttattgtcg aatgattgca taaccatctc
     2881 gaatgacctt cgcgccgtgg gatgatcgcg tacgccgccg tctcaaatta cgggacgtcg
     2941 atgttttgtt ggccgtggtc caaaccggca gcatgggcaa ggcgagcgcc gttctgcgca
     3001 tgtcgcagcc ggcggtctcc aaggcgatcg cctccctcga acagacactc ggggtgcagc
     3061 tgttggatcg gagccggcgc ggcgttgagc cgacgcccta cggccttgct ctcatcaaac
     3121 gcggcgtggc cgtgttcgat gagttgcgac agggcgttca ggatatcgcg tttctcaccg
     3181 atcctacagt tggcgagata cgtgtgggag gcactgaggg catgatctca acggtctttt
     3241 cgcccgtcgt tcaccaattg tcgacacaat atccgcggat gtcctttcgt gtcgtcgtcg
     3301 gagacttgcg aacgttatac cgagagttgg aggcacgacg cgtcgatgtc gtcgtcagcc
     3361 gactgcacaa tcctccttcc gaggagtatt cggccgaagt cctttatgag gataccctgg
     3421 tcgtcgtcac gggattgcgc aatccgctgg ctcgccgccg aaagatcgag attgccgaac
     3481 tgctggatga gccgtggaca ttccagccgt ccgacaccaa ttttggctcg ttcgccatgg
     3541 atgccttccg cgctgtcggg ctagctcctc cacggatcgc ggttgcgacc acatcccaca
     3601 ctctgcacaa cgaattgctg gcgacgggcc gctacctcgc gatggtccct cgcttttggg
     3661 tgctgctgca gcgtccgaac ccatcattca aagtcctgcc ggtggccttt ccccacaccc
     3721 aacacaaggt agcgattatt acgttgaaga accgatcgct cagttcggcc acgcagttct
     3781 ttatcgatcg cgtgcgtgcc atcacgaaag ccgtcaacga gcaaaaaaat aggccgatga
     3841 ctaaacgcgg caatccttca aaatctccgg cttgaacgcg aggccgtgac ccggtcgctc
     3901 cggcgcggtg atcatgccgt tcacgatcgg caccggatcg acgaacaaat cttccgacag
     3961 gcccatgtcc tcgacccagg ccgccgccga cgtcgtgccc atgaatccga cgactgacaa
     4021 tcctgtcggg cgttgtacgt ggtcggggat gctgtcttcg cgctcaaccg agtccgtatt
     4081 aataccttgg cgttaggcgc ccggttgccg acgatgcaca gcgggcgaga gtttgtcgag
     4141 gccggcggtc tgatgtcgta tgcaccgaac ttcccgaccc tgtcgcggcg cgctgccgaa
     4201 tttgtcgaca agattttacg cggggcaaag cccggcgaaa ttccgataga gcagccgacc
     4261 aagttcgatc tgatcgtgaa cctcatcacg gcgaaggcgc tcagccttga agtctcccca
     4321 acgctgctcg cccgcgccga caaggtgatc gaatagcggt gcttttttgc tgcaatgcga
     4381 atgtctgctt ttgtgaaggt tttcggatgc cggccgctga cggacggtgc cgcgcagcat
     4441 gggcggccgg cgtcggaaaa cctccaaggg aggaaaccgc gggaagcgag acgagggtgg
     4501 ttcgtcacgc actatgggga tttgagtgcc gcgaatgctg cgcgtgattg agtacgagac
     4561 gattccgggg cgtcgggcgg cggggatgcg cgtttgagtg agcggctggt gcgccgaact
     4621 gtgcgggcag ttgtgcggtt tgacagaaca accggacatt cggccgagcg tcgtcgctgg
     4681 cggcggagaa ccagcgaaca aagcgagcgc atttgcggca tcgtggcacg ggcactgtca
     4741 tgaagtgtcg atcctgatga tgggatctgt tggctgatgg cgcggcgtcg cgccgcgcgg
     4801 gaaggtctcg atgatgatca tgcggccgcc gcaacatgga catgccggct ggttgtgatc
     4861 ggtagcatcg gcgggttggc tttggggctt tggcccagcg agcagttcgc gagcacgcgc
     4921 gatgttgtcg gcgccggagg ttttggccaa caggccgtag tggcggatgc ggtggaagcc
     4981 cttgggcagg acgtggatga ggaatcggcg gatgaactcg tctgtggcga gtgtcatgcg
     5041 cttgtatcga tctcggccct cgatacggta gtccttgcac ttgaaggtga tgctgtcttg
     5101 gtcgaatgcg atcaagcggc tattggcgat ggcaacgcgg tgggtatagc gggagagata
     5161 ggccagcacc gcctcgggtc cgccgaacgg gcgctttgaa tagacgaccc attcggtc
//
LOCUS       DATLJT010000253         1830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1391390,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000253 DATLJT010000000
VERSION     DATLJT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391390"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..936)
                     /locus_tag="VM910_05765"
     CDS             complement(142..936)
                     /locus_tag="VM910_05765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI62087.1"
                     /translation="MSLFDAPFNPMQEAALVTGAGNGIGRAIAQALVGEGVKTVFADI
                     REDTVTAAAKASPRPDLAMPWVGDLADPAARDALLREAERAVGRVTHFVHSASPPRRE
                     ADHVFAVSGETWERMRAVNLDAGFHLTRALARKLIDAKQPGSFLLLTSLHAGTPRNLA
                     HYSTAKAAMAMLVKELAKTLGRFGIRVNALVPGAIAAGGFVADPALARHIPLGRLGKA
                     EDLAPMALAVLSNSISAYVTGAAIVVDGGISLTNWFDPPALEDFER"
     gene            complement(1099..>1830)
                     /locus_tag="VM910_05770"
     CDS             complement(1099..>1830)
                     /locus_tag="VM910_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62088.1"
                     /translation="LPFAISIVLVGVGLWIRLGILETPVFQKVLDEDKVEKAPIVEVF
                     KKHPKEIVLSALARMAEQAPFYIFTAFIFAYAVGTLHMSRNLILSAVLVASCVSFVSI
                     PLSGHISDLIGRRKMYLIGAATVGVFGFLYFAMVDTANPVLVFIAIVLSLIPHDMMYG
                     PQAALIAEAFTPRLRYSGASLGYQLASVIAGGPAPLIATALFASYKSGFAISIYIAIC
                     AVISLVSAAMMPDYTGQDVSKEYDH"
BASE COUNT          380 a          562 c          601 g          287 t
ORIGIN      
        1 cctgccacgc ttacgagcaa gcgcccgatg gctcgtggcg cgcgctcgcg tgcgaagaag
       61 tcggcggaaa cgggcagacc cagcacagga gccctgcgcg aagccgcgag gaagacccgc
      121 gctagccagc gcgcggtcgc cctatctctc gaagtcctcc agcgccggcg gatcgaacca
      181 gttggtcagc gatatgcctc cgtcgaccac gattgcggct cccgtgacat aggcggagat
      241 gctgttcgac agcacggcga gcgccattgg cgcaagatcc tcagccttgc cgagccggcc
      301 gagcgggatg tgtcttgcta gcgcgggatc ggcgacaaag cctcctgcag cgatggcgcc
      361 cggtaccagc gcgttgacac ggataccaaa acggccaagc gtcttggcga gttccttcac
      421 cagcatcgcc atcgcggcct ttgccgtcga ataatgcgcc aggttgcgcg gcgtgccggc
      481 gtgcagcgag gtcaggagca ggaacgatcc gggttgcttt gcatcgatca gttttcgcgc
      541 gagcgcgcgt gtaagatgaa agccggcatc cagattgacg gcacgcatgc gctcccaggt
      601 ctcgccagag acggcaaaga cgtgatcggc ctcgcggcgc ggcggagagg cgctgtgaac
      661 gaaatgtgtg acgcgaccga cagcacgctc ggcttcgcga agcaacgcat cgcgcgcggc
      721 gggatccgcg aggtcgccga cccagggcat cgcaagatcg gggcgcggtg aagccttcgc
      781 ggcagcggtg acggtatcct cgcggatatc ggcaaaaacg gtcttcacgc cctcgccgac
      841 cagggcctgc gcaatggcac gaccaattcc gtttccggcg ccggtgacta gcgccgcttc
      901 ctgcatgggg ttgaagggtg cgtcgaacag gctcatgtcg gactccgatg ccggttgggt
      961 cttgccggcc aatgaacctt cattcttcga ccggcgtcaa atgcttcaat ctatcttggg
     1021 accgcagccg cgggcggctg aaaaaaagag ggcctggatg cacccaagcc ctcctgaaag
     1081 ccgacgtgac cgacggagct agtggtcata ctccttcgag acgtcctggc cggtatagtc
     1141 aggcatcatc gccgccgaga cgaggctgat cacggcgcag atggcgatgt agatcgaaat
     1201 ggcgaagccg gacttgtagc tcgcaaacaa cgcagttgcg atcagcggcg cgggtccgcc
     1261 ggcaatcacg gaggcgagct gatagccgag cgaggcgccg ctgtagcgca gccgcggcgt
     1321 gaaggcttcc gcaatcagtg ccgcctgcgg gccgtacatc atgtcatgcg ggatcaacga
     1381 gagcacaatg gcgatgaaca ccaaaaccgg attggcggtg tccaccatgg cgaaatacag
     1441 gaagccgaac acgccgaccg tagccgcgcc gataaggtac atctttcggc gcccgatgag
     1501 gtcggagata tgaccggaca gcggtatcga gacgaaggag acgcaggagg cgaccagcac
     1561 ggcgctcagg atcaggttgc gcgacatgtg cagggtgccg acggcatagg cgaagatgaa
     1621 agcggtgaag atgtagaacg gcgcctgctc cgccatgcgc gccaatgccg agagcacgat
     1681 ctccttcgga tgcttcttga acacctcgac gatcggggcc ttctccacct tgtcctcgtc
     1741 caggaccttc tggaacaccg gtgtctcgag gatgccgagc cgaatccaca ggcccacgcc
     1801 gacgagcacg atcgagatcg cgaacggaag
//
LOCUS       DATLJT010000254         1879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4148727,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000254 DATLJT010000000
VERSION     DATLJT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4148727"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VM910_05775"
     CDS             complement(<1..576)
                     /locus_tag="VM910_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVI62089.1"
                     /translation="MQTKRLLLGMGVGLAVTFVPAALCFVRAQNQPPAALAGHVTSEA
                     EGAMEGVVVSAKKAGSTVTVSVVSDAQGRYSFPANRLSGGKYSLKIRAVGYDLIPPAS
                     ADVAEEQTTTVDLKLRKARNLASQMSNGEWMKSLPGTEDQKAFLLGCVGCHTLERIVR
                     STHDADEWTQVIVRMNGYGPVSQPIKPQRMLD"
     gene            complement(810..1646)
                     /locus_tag="VM910_05780"
     CDS             complement(810..1646)
                     /locus_tag="VM910_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI62090.1"
                     /translation="MHASLISILTIGAAALLAATGLAAPGLAPLFADNSATAVPVDVE
                     LVLAVDVSYSMDPDEQALQREGYIAGFTSREFMQALRSGQHGKVAVTYFEWAGPYDQK
                     IVVPWRLIDGPESADAFVNEIARASYRRASRTSISSALNFAKPLFDGSGYRGFRRVID
                     VSGDGANNSGPFVTIARDDVLAAGITINGLPIMLKRPNSFTMDIENLDIYYEDCVIGG
                     PGAFVVPIREREQFKEATRTKLVLEIAGRTPERRVIPAQARAPRISCTVGEQMWRDRW
                     GN"
     gene            complement(1718..>1879)
                     /locus_tag="VM910_05785"
     CDS             complement(1718..>1879)
                     /locus_tag="VM910_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HVI62091.1"
                     /translation="QWREVGLGAQILKDLSITSIRLLTSAKLTYVGLAGFGIEIVSTE
                     RVDDRTPRE"
BASE COUNT          348 a          596 c          579 g          356 t
ORIGIN      
        1 atcgagcatg cgctgcggct tgatcggctg gctcaccggc ccataaccat tcatgcgcac
       61 gatgacctgg gtccattcat ccgcatcgtg ggtcgagcgc acgatgcgct cgagcgtatg
      121 gcaaccgacg caaccgagca gaaacgcttt ctgatcctcc gtgcccggca ggctcttcat
      181 ccattcgccg ttgctcatct gcgaggccag gtttctcgcc ttgcgcagtt tgagatcgac
      241 ggtggtggtc tgctcttcgg cgacgtccgc gcttgccggc gggatgaggt cgtagccgac
      301 agcgcggatc ttgagcgaat acttaccgcc gctcaatcga ttggctggaa agctgtagcg
      361 gccttgggcg tcgctgacga cgctgacggt gaccgtcgag cccgccttct tggcgctgac
      421 caccaccccc tccatcgcgc cttccgcttc ggaggtgaca tgtccggcaa gggcggccgg
      481 cggttggttt tgcgctcgca cgaaacagag cgccgccggc acgaacgtca cggccaaacc
      541 gacgcccatg cccagcagta aacgttttgt ttgcatcgat tcctcccgac ggagtcgggt
      601 cacaattcga ggacacatct cccagatgaa gcatcgtaga atgcgtggcg gttgaaatcc
      661 agagcctcct caggcttgca cgaagctgtg gaagcgatct ctctcagcgg gcggcgccaa
      721 acctcgccag caactgcttt ggtcaatgcg cgcttcgccg ctcggcaacg aacgccggtc
      781 gtcaatcgca gcttgaggaa ttccgcggcc tagtttcccc agcgatcccg ccacatctgc
      841 tcgccgacgg tgcaggagat gcgaggggct cgcgcttgcg ccggtatcac gcgtcgttcg
      901 ggcgtgcggc cagcgatctc gagcaccagt ttggtacgcg tcgcttcctt gaattgctcg
      961 cgctcgcgga tggggacgac gaatgcaccg gggccgccga tgacgcagtc ctcgtagtaa
     1021 atatcgaggt tctcgatatc catggtgaac gagttcggcc gcttcaacat gatcggcagg
     1081 ccgttgatgg taatgccggc agcaagcacg tcgtcgcgtg cgatggtgac gaacgggccg
     1141 ctgttgttgg cgccgtcgcc tgagacgtcg atgacgcgtc gaaagccgcg atagccgctg
     1201 ccgtcgaaaa ggggtttggc aaaattaagc gcgctcgaaa tcgaggtgcg cgaggcacga
     1261 cggtaagacg cgcgggcaat ctcgttcacg aatgcatcgg cgctctccgg tccatcgatg
     1321 agccgccatg gcacgacgat cttctgatcg tagggtcccg cccactcgaa ataggtgacc
     1381 gccactttgc cgtgctggcc gctgcgcagc gcttgcatga actcgcgcga cgtgaagccg
     1441 gcgatgtagc cttcgcgctg cagcgcctgc tcatccgggt ccatcgagta ggacacgtcg
     1501 acggccaata cgagctcgac atcaaccggc accgcagtcg cactgttgtc ggcgaacaga
     1561 ggcgccaagc ccggtgcagc caatccggtg gccgccagca gcgcggcagc accgattgtc
     1621 aggatcgaga tcagcgacgc atgcatccgt ccctccgtca gcggacgatt ctcccatggt
     1681 gacatgggca ctttgacaaa gcgaccgcgc ggcacgatca ttcccgcggc gtcctgtcat
     1741 cgaccctttc ggtcgacacg atctcaatcc cgaagccggc gaggccgaca taggtgagct
     1801 ttgcggaagt cagcaagcgg atcgaggtga tgctcaagtc cttgagaatc tgggcgccga
     1861 gcccgacctc gcgccactg
//
LOCUS       DATLJT010000255         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2310559,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000255 DATLJT010000000
VERSION     DATLJT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310559"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VM910_05790"
     CDS             <1..779
                     /locus_tag="VM910_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HVI62092.1"
                     /translation="RLAKERPDCLRSVTLLAAQTDFTEAGELTLFINESQVAFLEDMM
                     WERGVLDTAQMAGAFQILRSNDLIWSRAVRDYLLGEREPLSDLMSWNADATRMPYRMH
                     SEYLRKLFLNNDLAEGRYSVEGQPVALSDLHAPMFVVGTQRDHVAPWRSTHKIHFLAA
                     DADITYVLTSGGHNAGIVAPPAEEGHTYQIMAKKADGAYVGLDEWIKLAPQQVGSWWK
                     EWVSFLENHSGEAVAPPIMGGVRQKAASLKDAPGDYVLQR"
     gene            complement(807..2330)
                     /locus_tag="VM910_05795"
     CDS             complement(807..2330)
                     /locus_tag="VM910_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI62093.1"
                     /translation="MTPPANERRDPSQEPVLAFLDNGARKRIDTHASVVFLEPDRVLK
                     VKQAVRLPFLDFSTLDKRKHACEEEIAVNKRHAPMLYRRVVPITKGARGPEISGSGEV
                     IEWAVEMARFDEQQTFDHLAVGGAITPELGEALADVMLIAHKTAPVSHGSIWPASIAG
                     IIDRNTAKFHAQKNLARDEVQRLHAASHRQMDEHLPLMRRRAADSQIRHCHGDAHLGN
                     IVLIAQKPVLFDAIEFDPDIATTDVLYDLAFPLMDLVRHAQDAAANRLFNRYLQETWA
                     EQADALVLLPLFMSMRAAIRAHVLFTRQEQSESDAAIATQARSYFDLALRLISPSPPA
                     LVAIGGRSGTGKSLLARGLAPMLEPAPGALVLRSDVIRKALFGVDPLTALPAAGYAPD
                     VTTRVYHLMAQRAATALQQGVSVVLDAAFLRQGERDPLPNLARASGARFRGIFLDADV
                     TARLGRIAARGRDASDATREVALQQQSYDIGRLDWDIVDASGSPQDTLERATARRRT"
     gene            complement(2327..>2846)
                     /locus_tag="VM910_05800"
     CDS             complement(2327..>2846)
                     /locus_tag="VM910_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVI62094.1"
                     /translation="LVMAGSGKTLGIYGFGAAGHIVAQVARWQGRTVYAFTRKGDTEA
                     QQLARSLGADWAGASEDIPPVELDAAIIYAPVGALVPQALRAVRKGGKVVCAGIHMSD
                     IPSFPYNILWGERQIVSVANLTRKDGTDFLKVAHEAGVRTHTTSFPLEQVNEALTRLR
                     DGKLVGAAVLRP"
BASE COUNT          510 a          890 c          940 g          506 t
ORIGIN      
        1 cgcggcttgc caaggagcgg ccggattgcc tgcgttcggt tacgcttttg gccgcgcaga
       61 ccgattttac cgaagcgggc gaactcacgc tgttcatcaa cgaaagccag gtcgccttcc
      121 tcgaggacat gatgtgggag cgcggtgtgc tcgataccgc gcaaatggcg ggcgcattcc
      181 agatcctgcg ctccaacgac ctgatctggt ctcgcgcggt gcgcgactac ctgctcggcg
      241 agcgcgaacc gctcagcgac ctgatgtcgt ggaacgccga tgcgacgcgg atgccctatc
      301 gcatgcattc ggaatatctg cgcaaattgt tcctcaacaa cgatctcgcc gagggccgct
      361 atagcgtcga aggccagccg gtggcgctgt ccgatctgca cgcgccgatg ttcgtcgtcg
      421 gaacgcagcg cgaccatgtc gcgccgtggc ggtcgacgca caagatccat ttcctcgccg
      481 ccgatgcgga tatcacctat gtgctgacca gcggcgggca caatgcggga atcgttgctc
      541 cgcctgcaga agagggacac acctatcaga tcatggccaa gaaagccgac ggtgcctatg
      601 tcggtctgga cgagtggatc aagcttgctc cgcaacaagt gggatcgtgg tggaaggagt
      661 gggtaagctt cctcgaaaac cactccggcg aagcggtcgc gccgccgatc atgggcggcg
      721 tgaggcaaaa ggctgcgtca ctcaaggatg cgcctggaga ttatgtgctg cagcgctgaa
      781 gcgacatcgc gctttgcagg acaagtctac gttcgccgac gggccgtcgc ccgctccagc
      841 gtgtcctgcg gcgagccgga ggcatcgacg atgtcccagt cgagacggcc gatatcgtag
      901 ctctgttgtt gaagcgccac ttcgcgcgtg gcgtccgagg cgtcgcgtcc acgcgcggcg
      961 atgcgtccca gccgcgccgt gacatcggcg tcgagaaaga tgccgcgaaa gcgcgcgcca
     1021 ctcgcgcgtg cgaggttcgg cagcggatcg cgttcgccct gccgcaggaa tgccgcgtcg
     1081 agcacgaccg agacaccctg ctggagcgcg gtcgccgcgc gctgcgccat gaggtgatag
     1141 acgcgggtcg tgacatcagg cgcatagccg gctgcgggga gagccgtgag cggatcgacg
     1201 ccaaaaagtg ccttgcggat cacgtccgag cgcaagacca gggccccggg cgcaggttcg
     1261 agcattggag cgaggcctcg cgcaagcagg ctcttgccgg tgccggaccg gccgccgatc
     1321 gcgaccagcg ccggcggcga cggcgatatc aggcgaagcg cgaggtcgaa ataggatcgc
     1381 gcctgtgtcg cgatggcagc atcgctttcg gattgctcct gcctggtaaa gagcacgtgc
     1441 gcgcggattg ccgcccgcat cgacatgaac agcggcaaca acacaagcgc gtccgcctgc
     1501 tcggcccagg tctcctgcaa atagcgattg aacagccggt tcgccgcggc gtcctgtgcg
     1561 tgccggacaa ggtccatcag cggaaaggcg aggtcgtaca ggacgtcggt cgtcgcgatg
     1621 tcgggatcga actcgatggc gtcgaacagc acgggtttct gcgcgatcag gacgatgttg
     1681 ccgagatgcg catcgccgtg acagtggcgt atctggctgt ctgcggcacg ccgccgcatt
     1741 agcggaagat gctcatccat ctgtcgatga cttgccgcat gaagtcgctg gacctcgtcg
     1801 cgagcgaggt ttttctgcgc gtgaaatttt gcggtgttgc gatcgatgat gccggcaatc
     1861 gaagcgggcc agatcgatcc gtgcgacacc ggcgccgtct tgtgcgcaat gagcatcaca
     1921 tctgccagcg cttcgcccag ttccggcgtg atggcgccgc cgacagcgag atgatcgaaa
     1981 gtctgctgct cgtcgaaccg cgccatctcg accgcccatt cgatcacctc gccgctgccg
     2041 gagatctcgg gtccacgtgc acctttcgtg atcggcacga cgcggcggta cagcatcggc
     2101 gcatggcgct tgttgaccgc gatctcttcc tcgcaggcat gcttgcgctt gtcgagtgtc
     2161 gagaaatcca gaaagggcag gcggaccgct tgcttgacct tcagcacgcg gtcgggttcg
     2221 aggaagacga cggaggcgtg ggtgtcaatt cgcttgcgcg cgccattgtc gaggaacgcg
     2281 agcaccggct cctggctggg gtcacgacgc tcgttcgccg ggggcgtcac gggcgcagca
     2341 ccgcggcacc gacgagcttg ccgtcgcgca ggcgtgtcag cgcctcattg acttgttcaa
     2401 gcggaaagct tgtcgtatgc gtccgcacgc cggcttcgtg agcgaccttg aggaaatccg
     2461 tgccgtcctt gcgcgtcaaa ttcgcgacgg agacgatctg ccgctcgccc cagagaatat
     2521 tgtaagggaa cgaagggata tcggacatgt ggatgccggc gcagaccacc tttccgcctt
     2581 tgcgtactgc gcgcagcgct tgcggcacca gcgccccgac cggcgcatag atgatggcgg
     2641 cgtcaagctc gaccggcggg atgtcttcgg atgctcccgc ccagtcggct ccgagcgaac
     2701 gtgcaagctg ttgtgcctcc gtatcgccct tgcgcgtgaa cgcatagacc gttcgtccct
     2761 gccagcgcgc aacctgtgcg acgatgtgcc cggccgcgcc aaagccatag atcccgagcg
     2821 tcttgccgga gccggccatg accagc
//
LOCUS       DATLJT010000256         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2310725,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000256 DATLJT010000000
VERSION     DATLJT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310725"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VM910_05805"
     CDS             <1..328
                     /locus_tag="VM910_05805"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HVI62095.1"
                     /translation="VWRPIRHPVESFPLAICDARSISPRDFVVSERRYPNRVGQTYAI
                     TYNPEHRWYWLPRMRREEALVFKVYDSSKDGRARWSAHTAFEDPTSPPNARPRESIEI
                     RTLAFF"
     gene            714..1661
                     /locus_tag="VM910_05810"
     CDS             714..1661
                     /locus_tag="VM910_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62096.1"
                     /translation="MACLIVLAAATADAALGQPVTLRIGFGMAAEEPLWVLVAKPDLA
                     KNHGKAYALDATRFTGSDKRSQAFEAGAIDLAASSANGVIFAAAEGVAAKMIASISRE
                     SPRGFSTSFYVKDDSPIKSVADLKGKIVGINGFYTSGHLWLKTALEKQGLAETDVTIT
                     PVPFPAMQEALEAGKVDLGMFPQPFAALAEKQAKVRKIFDAKYGIPFEEELIVLIGKD
                     EFLKKNAGAVRAFLEDLKVSMQFYLEKPREARQLLIDHKMVRVNPDVYLAMNDYYRDP
                     TLRVDVEALEKMQAVQIAAGFQKKRADVSSLVDLGHLPK"
     gene            1674..2468
                     /locus_tag="VM910_05815"
     CDS             1674..2468
                     /locus_tag="VM910_05815"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62097.1"
                     /translation="MPATMPLLEVTDLDKTFTVDSRSVRAIDHLSLSIDEHEFVSIVG
                     PSGCGKSTFLHIIGGFEPATAGELRLSGRPIGPPGPDRGMMFQELSLFPWLTAIENVA
                     WPLEMKGLPKRERTAKAAEYLDLVHLSRFADVYPGQLSGGMKQRVALARLFALDPEIM
                     LMDEPFGALDSQTRELLQEELQSIWRRARKTALFVTHDIDEAIYLGTRVIVFTARPGR
                     IKADIRIPNLDRSGDYRKSREYLALRVEVWDLVRDEVLKARALEEA"
     gene            2465..3241
                     /locus_tag="VM910_05820"
     CDS             2465..3241
                     /locus_tag="VM910_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62098.1"
                     /translation="MRLNSASIQHWILPVAALAAWEILGRAGLLPRYLSTPSAILAAL
                     WEIALTGELFVALAASLYRVSVGFALGTAAGIVVGLGAGILPGVRHFFDPLVSFLYSI
                     PKVAFLPIFLLLFGLGHASKIAIIAFSGFFPVFVASRHAVLSVDKLLLWAARNMGAPS
                     RTIFFRVVIPAAAPQLFGGIRIGLAHAFVVLFAAELIGSKVGLGTIISYGEEWVRFDL
                     MFAGIVCFAVLGFLGDRMLLAVRSRVLKGQMLGTEEEAVR"
     gene            3238..>3909
                     /locus_tag="VM910_05825"
     CDS             3238..>3909
                     /locus_tag="VM910_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62099.1"
                     /translation="MTAQTFAQARSLIGRWYSIPLLLVVWQVAVASGLVESRLLPSPG
                     RVWTALATEVGNGTLIYHAGVTVSRALAGFLLAALVGIPFAGAMGRSVLVRNLFEPIF
                     FIGYPVPKIALFPVFTYIFGIGTPSKIAFTFLECLYPVVVTCYFGFRGVQTRLIWSAQ
                     NCGASRSIILRRVILPATLPSIFSGLRVALPVAIIVVVITEMIGDSIGLGYYITIWST
                     RFTFAN"
BASE COUNT          661 a         1198 c         1218 g          832 t
ORIGIN      
        1 ggtgtggcgg ccgatccgcc atccggtcga gagcttcccg ctcgcgatct gcgacgcgcg
       61 cagcatctcg ccgcgggatt tcgtggtgag cgagcgccgc tatcccaacc gggtcggcca
      121 aacctacgcc atcacctaca atcccgagca ccgctggtac tggttgccgc gcatgcggcg
      181 cgaggaggcg ctggtattca aagtctacga ctcttcgaag gacggccgcg cccgctggtc
      241 cgcccacacc gctttcgagg acccgacctc gccgcccaat gcgcggccgc gcgagagcat
      301 cgaaatccgc acgctggcgt ttttctgaga gcccgcgacg acgcagccgc gcaagctcgt
      361 gcgcgcctga gcgcggcggt tgttggcgag cctcgagtgg ctcgcgcccc gccaaattcg
      421 agctgacgcc gttctgacgg cgccattttg ccgccattcg ccaagtggcc atctcaaagc
      481 cgcactttcc aatcccaaag ccctgccgtg cggcggtcat cgagatcgcg cgttgcttca
      541 tgccgtcccg ccatcgcgac gaatcgcagc ttgttctgcc gccttcccgc tcgaaaaatg
      601 tgtttgcagc gaatgatcga gccgatacgc tttgtcgcca agttggccgt tcacgcttag
      661 gcgaaccggg gctcgacatg ggcggatttg acgcgaaggc cgcggcgcgg cgaatggcat
      721 gcttgatcgt gcttgcggcc gcgacggccg acgcagccct cgggcagccg gtcacattgc
      781 gcatcggctt cggtatggcg gcggaagaac cgttgtgggt gctcgttgcc aagcccgatc
      841 tggccaagaa ccacggcaag gcctacgcgc tcgacgcgac gcggtttacc ggctcggaca
      901 agcgcagcca ggcgttcgag gccggtgcca tcgacctggc cgccagcagc gcgaacggcg
      961 tgattttcgc ggcggcggaa ggcgtggcgg ccaagatgat cgcgtcgatt tcccgcgaaa
     1021 gcccccgcgg cttcagtaca agcttctacg tcaaggacga ttcgccgatc aaatcggtgg
     1081 ccgacttgaa gggcaagatt gtcggcatca acggcttcta cacgtccggt cacctttggc
     1141 tgaaaaccgc gcttgaaaag caagggttgg ccgagaccga tgtcacgatc acgccggttc
     1201 cattcccggc gatgcaggaa gcgctcgaag ccggaaaggt cgatctcggg atgtttccgc
     1261 agccgttcgc cgcgctcgca gaaaagcagg cgaaggtccg caaaatcttc gatgccaaat
     1321 atggcatacc attcgaggag gaactgatcg tcctcatcgg aaaggacgaa ttcctgaaaa
     1381 agaacgcggg tgcggtgcgc gcatttctcg aagatcttaa agtgagcatg cagttctatc
     1441 tcgaaaagcc ccgcgaagcc cggcaactcc tgatcgatca taagatggtt cgcgtgaacc
     1501 ccgacgtcta ccttgccatg aacgactact atcgcgaccc gacgttgcgg gtcgatgtcg
     1561 aagcgctcga gaagatgcag gcagtccaga tcgcggccgg gttccagaaa aagagggccg
     1621 acgtgagctc gctggtggat ctcggtcatc tgccgaaatg agcttgctca agaatgcccg
     1681 cgaccatgcc gctgttggag gtcacggacc tcgataagac gttcacggtc gacagccgca
     1741 gcgttagagc catcgatcat ctctctttgt cgatcgacga gcacgaattc gtctccatcg
     1801 tcgggccttc gggctgcggc aagagcacgt tcctgcacat catcggcggc ttcgagcctg
     1861 cgaccgccgg tgagctgcgg ctgagcggac ggccgatcgg ccctccaggt cccgatcgcg
     1921 gcatgatgtt tcaggagctt tcgctatttc cttggctcac cgccatcgag aacgtcgcat
     1981 ggccgctcga gatgaaggga ttgccgaaac gcgagcgtac cgccaaggcg gccgaatatc
     2041 tcgatctcgt tcatctttct cgtttcgccg acgtctatcc cggccaactg agcggcggaa
     2101 tgaaacagcg cgttgccctg gcacggctgt ttgcgctcga tccggaaatt atgctgatgg
     2161 atgagccgtt cggcgcgctc gattcccaga ctcgggaatt gctgcaggag gagctgcagt
     2221 cgatctggcg gcgcgctcgc aagacggcgc tgttcgtgac ccacgacatc gacgaggcga
     2281 tctatctagg aacgcgggtg atcgtgttca cggcgcgtcc aggccgcatc aaggccgaca
     2341 tccgcattcc caatctcgat cgttccggtg actaccgaaa gtcccgggaa tacctcgctt
     2401 tgcgggtcga agtctgggat ctggtgcggg acgaagtatt gaaggcgcgc gcgctggagg
     2461 aagcatgaga ctcaactcgg cttcgatcca gcattggatt ttgccggtcg cggcgttggc
     2521 ggcttgggaa attctcggcc gcgccggcct gctgccgcgc tatctgtcga cgccgagcgc
     2581 gatccttgcg gccctgtggg aaatcgcgct gacgggcgag ctcttcgtcg cgctggccgc
     2641 gagcctctat cgggtgtcgg tcgggtttgc cctcggcacg gctgccggca tcgtggttgg
     2701 ccttggcgcg gggattctgc ccggtgtgcg ccatttcttc gatccgctgg tgtcctttct
     2761 ctactccatc ccgaaggtcg cgtttctgcc gatctttctg ctcctgttcg gcctcgggca
     2821 tgcatccaag attgccatca tcgcattttc aggtttcttt cccgtgttcg tggcctcgcg
     2881 tcacgcggtg ctgtccgtcg acaagcttct gctctgggcg gcgcgcaata tgggagcgcc
     2941 gtcgcggacg atctttttcc gcgtcgtcat tccggcggcc gcgccgcagt tgttcggcgg
     3001 catccgcatc gggctcgcgc atgccttcgt ggtgctgttt gcggccgagc tgataggctc
     3061 gaaggttggg ctcggtacga tcatctccta cggcgaggaa tgggtgcggt tcgatttgat
     3121 gttcgctggt atcgtctgct tcgccgtgtt gggatttttg ggcgaccgca tgttgctggc
     3181 ggtccgatcg cgcgtgctca agggccaaat gctcggcacg gaggaagagg ccgtgcgatg
     3241 accgctcaaa ccttcgcgca agcccggtcg ctgatcgggc gctggtactc gatcccactg
     3301 ctgcttgtgg tttggcaagt ggcggtcgcg agcggtctcg tggaatcccg tctgcttccg
     3361 agcccgggga gggtttggac cgcgcttgcg acggaagttg gcaatgggac gctcatctac
     3421 catgcaggcg tgacggtttc gcgcgctctc gccggcttct tgctcgcggc gctcgtcgga
     3481 attccattcg ccggtgcgat ggggcgatcc gtgctagtgc gcaatctgtt cgagccgatt
     3541 tttttcatcg gctatccagt accgaagatc gccttgtttc ccgttttcac ctatatattc
     3601 ggcatcggaa cgccctcgaa gatcgcgttc acctttctcg aatgcctcta tccggtcgtg
     3661 gtgacgtgct atttcggctt tcgcggggtc cagacgcggc tgatttggag cgcgcaaaat
     3721 tgcggcgcaa gccgatccat aattctgcgg cgggtcattt tgccggcgac gctgcccagc
     3781 attttctcgg ggctgcgtgt cgcactgcct gtggcgatca tcgtcgtcgt catcaccgaa
     3841 atgatcgggg attccatcgg gcttggatat tacatcacca tctggagcac gcgcttcacc
     3901 tttgccaac
//
LOCUS       DATLJT010000257         2705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2311143,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000257 DATLJT010000000
VERSION     DATLJT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311143"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(296..856)
                     /locus_tag="VM910_05830"
     CDS             complement(296..856)
                     /locus_tag="VM910_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI62100.1"
                     /translation="MSKENPLWGAPRIHGELLKLGFEVAESTVSKYMIRRRGPPSQTW
                     RTFLRNHAAAIAAIDLCLVPTLTFECLFAFLVLGHSRRQLLWFAVTRHPTAEWLAQQI
                     VEAFPWGAAPTYLVRDNDRAYGQAFTNRVRAMGIRDRPISPRSPWHNPYAEQLIGTLR
                     RDCLDHVLIFNERHLRGVLTLYSLYY"
     gene            complement(1262..1948)
                     /locus_tag="VM910_05835"
     CDS             complement(1262..1948)
                     /locus_tag="VM910_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI62101.1"
                     /translation="MTNTRKILIVDDDFELREALVGQLALHEEFESIAVDNGTKGVQT
                     AKAGQIDLVIMDVGLPDVDGREAVRILRKNGFKAPIIMLTGHNTDSDTILGLESGAND
                     YVTKPFRFAVLLARIRTQLRQHEASEDAVFTIGPYTFRPSSKILLNPKGNEVLLTEKE
                     TAVLRYLYRAGQRPVSRETLLQEVWGYNSGVTTHTLETHIYRLRQKVERDAASPAILV
                     TEAAGYKLVP"
     gene            complement(1970..2413)
                     /locus_tag="VM910_05840"
     CDS             complement(1970..2413)
                     /locus_tag="VM910_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62102.1"
                     /translation="MTSLALVSDRQPAIAAPPVHWAHDVRNVLASVGLHLDTLARLSG
                     PHGAKAAYAGHALISKVSALCNVALIGDPNGCSQRQPFEITAMIRHVVDLLAPAAPDG
                     FRVNIVSDAPAYVLADQNEIFRVLFNLLHNALTVARAAPASAVPG"
BASE COUNT          558 a          797 c          830 g          520 t
ORIGIN      
        1 ggatacgaaa aagggatccc ctgtctgtag ctagtggacc aagcgtgttg gattaattaa
       61 atcccgcata agggacaggt aactgagctc gtcgcggagt tctggatgct cacgctgtaa
      121 ggctgcattt ctctcaaata gcttcgccgc cgcggctgtc cttcccgaaa atcatatgcg
      181 cacgtagcaa tgatgcaatc cggacaagat tggttcggtt acgatagccc cagatcgttg
      241 gacggctcgt ggtagcggcg cgtccttccc caatcccaaa tgcgtgcggg tctcattagt
      301 aatagagcga atacaaagtc aggacccctc gcaaatgccg ttcattgaag atcaggacat
      361 gatccaaaca gtcgcgacgg agtgtcccga tcagttgctc cgcataaggg ttgtgccagg
      421 gcgacctcgg tgagatcggg cggtcgcgaa tccccatcgc ccgaactcta ttcgtgaatg
      481 cctgtccata ggcgcgatca ttgtcgcgca ccaagtaggt cggtgccgcg ccccacggga
      541 atgcctccac gatctgctga gccagccact ccgcagtcgg gtgccgggtg accgcgaacc
      601 agagcagctg tcgtcgacta tggcccaaca cgagaaatgc aaacaagcac tcgaacgtca
      661 gggttggaac caggcagagg tcgatcgccg cgatggcggc cgcatggttg cgaaggaatg
      721 tccgccaagt ctgcgatggc ggccctcggc gccggatcat gtatttggac acggtcgact
      781 cggcgacctc aaacccgagt ttgagcagtt caccatggat gcgcggggcg ccccacaatg
      841 ggttctcctt gctcatgcgt tggatgagtt cgcgcagctc acggctaact ctaggtcttc
      901 ctgcccgacc gcgagatttc cagcgccaat aggcccgaaa ccctgcgcga tgccagcgca
      961 ggatcgtgtc gggctgaacg acgtgggaca aaccgagcag gccgggccag atccaagtct
     1021 accaataacg cccggtcgct cccacgaagc cgtagacggt acggcgcgcc cctcagagca
     1081 atattgagtt gatgccggag aaagaggttc tcgacttcaa gccggcgttg cgacttgaat
     1141 aggttggcga caaacgtccc aagaagatgg aggatggcaa gcatcgcgcc ggatgatgct
     1201 ctgattctcc ctcattgcgc gaatccgggc cggatggaat tttcgagaac gacaggggcg
     1261 gttacggcac caatttgtaa ccggccgctt cggtaaccag gatggcaggg cttgcagcgt
     1321 ccctctcgac cttctgccgc agccgataga tgtgcgtctc aagcgtgtgg gtggtgacgc
     1381 cggagttata gccccagacc tcctgcaaca gcgtctcgcg cgagaccgga cgttggccgg
     1441 cacgatagag atatcgcagg accgcggtct ccttctccgt gagcagcacc tcgttgccct
     1501 tgggattgag caggatcttc gagctcggcc ggaacgtata ggggccgatg gtgaacacgg
     1561 cgtcctcgct cgcctcgtgc tggcgtagct gggtgcggat acgcgcgagc agcacggcaa
     1621 agcgaaacgg cttggtcacg tagtcgttgg cgccggactc gagcccgaga atggtgtcgg
     1681 aatcggtgtt gtgtccggtc aacatgatga tgggcgcctt gaagccgttc ttgcgcagga
     1741 tgcgcacggc ttcgcgcccg tcgacgtctg gaagcccgac atccatgatc acaagatcga
     1801 tctgcccggc cttggcggtc tgcaccccct tggtgccatt gtcgaccgcg atcgattcga
     1861 actcctcgtg caaggcgagc tgtccgacca gcgcctcgcg cagttcgaag tcgtcgtcca
     1921 ctatcaggat cttgcgggta ttcgtcattc ctcggcccca ggatcggcat caccccggaa
     1981 cggcgctcgc gggagccgca cgtgcgaccg ttagggcatt gtgaaggaga ttaaacagca
     2041 cgcggaagat ttcgttttga tctgcgagca catacgcagg cgcgtcgctc acgatattga
     2101 ccctgaaacc gtccggggca gccggcgcca gaagatcaac gacgtgccgt atcatggcgg
     2161 tgatttcgaa cggctgccgc tggctgcaac cattcggatc gcctatcagg gcgacgttgc
     2221 acaatgcgct caccttggaa ataagcgcat ggccggcgta ggccgccttg gcgccgtggg
     2281 ggccggacag gcgcgcaagt gtatccagat gaagcccgac gcttgcgagc acattgcgaa
     2341 catcgtgggc ccaatggacg ggcggcgccg cgatcgccgg ctggcggtcg gaaacaagcg
     2401 ccaaactggt caatctaccc tccacgctcg gcgggcgcga acctggtttg tcaggcgcgt
     2461 ctggcgatga agaaattgag gtgcccttgc ctcctctcgg acccatcata ccggctcact
     2521 cgccctcgaa tgtgcatggg ccaatgaagc gcaggaacag ctcgatgtga aataccaatt
     2581 cgcaatggat ttgatagctg ccgtctatgg gatcggcggc agggcgaaaa acccggcgct
     2641 ggtggcactc agatggctgc gcagcgccgc cacgacgagc tgtgtgtcct cataacttga
     2701 tccac
//
LOCUS       DATLJT010000258         3261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_702190,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000258 DATLJT010000000
VERSION     DATLJT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702190"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VM910_05845"
     CDS             complement(<1..218)
                     /locus_tag="VM910_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI62103.1"
                     /translation="MAHDNGRKLVFQLTGAFAEFERTMIRQRIKAGLKRAVAPGREAR
                     PAEDRWRDRTEGAKAACERRGHLEGGEVA"
     gene            complement(284..1252)
                     /locus_tag="VM910_05850"
     CDS             complement(284..1252)
                     /locus_tag="VM910_05850"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI62104.1"
                     /translation="MPLHTKLTEKLGISHPILLAPMGTASGGTLAKAVSEAGGLGIIG
                     FGYGNQERIDQEFRAAGNARVGCGFITWSVARQPHLLARALDHKPVAIMLSFGDARPF
                     AAAIKDAGAALISQVQTVAQAMEAVDIGTDIIVAQGTEAGGHGAARSTLALVPAVVDA
                     VRKRNDEIVVVAAGGIADGRGLAAALMLGAQGALLGTRFLASDEALTSAAAKAKLVAA
                     RGDDTVRTRVFDIVRNLDWPALYTGRALQNAFSRQWHGRDAELEKSLPAEAQRYTRAA
                     EANDLDTAVVFAGECVDLIHDVKPAAAIVAALVHEAEAALRSNLSR"
     gene            1997..2449
                     /locus_tag="VM910_05855"
                     /pseudo
     CDS             1997..2449
                     /locus_tag="VM910_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2502..2975)
                     /locus_tag="VM910_05860"
     CDS             complement(2502..2975)
                     /locus_tag="VM910_05860"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HVI62105.1"
                     /translation="MPSNLWEPGQSGNPAGRLRGSRNKLSEEVICALLRDFREHGQKA
                     IAKVRRTQPAAYLKVLALLVPREHKVEHSNSLKNWTDEELEAGIDLVKQMLESGAAAS
                     GAVIEGTAEPVGMLGPAELEQPQHKRSNRLLEHVDTAVAPKECKPRKRVPSPLDT"
BASE COUNT          629 a         1081 c          927 g          624 t
ORIGIN      
        1 gcgacctcgc caccttcaag atgcccacgc ctttcgcaag ctgctttcgc accttccgtt
       61 cggtcgcgcc atctatcttc ggccggccga gcttcacgcc ctggggcgac ggcgcgcttg
      121 agccctgctt tgatccgttg gcggatcatc gtccgctcaa actcagcaaa ggcgcctgtt
      181 agctggaaca cgagcttgcg cccattatcg tgggccatcg ctctggtttt cctgcggcag
      241 ggcgagcacc cgtttaccgc gccagcgatc aagctcacgc catctatcga gacaggttag
      301 acctaagtgc ggcctccgcc tcatgaacga gcgccgccac gatcgccgcg gctggcttta
      361 cgtcgtgaat gagatcgacg cattcacccg cgaaaaccac tgcggtatcg agatcgtttg
      421 cttcggctgc ccgtgtgtag cgctgtgcct cagcggggag gctcttctcc agttccgcgt
      481 cgcgcccatg ccactgccgt gaaaacgcat tctgcaacgc ccgcccggtg tatagggcgg
      541 gccaatcgag attgcgtacg atgtcgaaga cgcgcgtacg gacggtgtcg tcgccgcggg
      601 ccgcgacgag cttcgccttg gccgcggcgg aagtcagcgc ctcgtccgag gcgaggaaac
      661 gggtgccgag aagggcgccc tgcgcgccaa gcatcaacgc ggcggcaagg ccgcgcccgt
      721 ccgcgatccc gccggctgcc accaccacga tctcgtcgtt gcgcttccgc acagcgtcaa
      781 cgactgccgg cacaagcgcc agagttgagc gcgccgcgcc gtgcccgccg gcctccgtac
      841 cctgcgcgac aatgatgtcg gtgccgatat caaccgcttc catcgcttgc gcgacggtct
      901 gtacctggga gatgagcgct gcgcccgcgt ctttgatcgc cgcggcaaaa ggtcgggcgt
      961 caccgaagga gagcatgatg gctacgggct tgtgatccaa ggcccgggct agaagatgcg
     1021 gctgacgagc gactgaccac gtgatgaacc cgcagcccac gcgagcattg ccggccgccc
     1081 gaaattcctg atcgatccgc tcctgattgc cgtagccaaa accgatgatg ccaaggccgc
     1141 ccgcttcgct gaccgccttt gccagcgttc ccccggacgc tgttcccatc ggggcaagaa
     1201 gaatcggatg gctgatgccg agtttctcgg tgagctttgt gtgaagcggc atcgagtttg
     1261 ttccttctat tcgtggcttg cgctcgatgc cgtcgatcag aaatcatcca accgctccgt
     1321 ccaatacacg caggacggtt caagacacaa cgatctttca gcaccgattt cgacattctc
     1381 acggaacgga tagaccgtgg gcaaccgatg atgcgtcgct agcgtgacga gttgcacgcg
     1441 acggctgtcg aacaggggat cgggactgac catgagcgcg tcggctcgct tttgtaatag
     1501 gtttacgaag gctgcatcaa tctcacgaga gctgctggca ttttttccga aaggaggtag
     1561 cccaaaaaca gcccacccca ttccgcacca gcaacctccc tgaccagaaa tagttcacac
     1621 caaatttccg gggatgaggg ctggatgaaa atttgaatcc gaaaagtccc ccctaccccg
     1681 ggggcagagc gcatgcgcac ctcgaggctc gcgccgccgc ctcatgccgc acgcctgccc
     1741 ccagccgcct ccgtcctgca tcgatcacga tgcgttccat gcggcgttcc gcccctctaa
     1801 actgtgcaag aaaggcgtat gttatataca gtagcacacg cgcaggcaaa gtttatccgc
     1861 atgaaatcga catccaaaaa acgcacacca agcatcgtaa acctgacagt caagcggcgc
     1921 tacctcaccc agcgcgaggt cgagcggctg ataagactgt gcccgcaagc atggccgcta
     1981 cgggcatcgc gatgcgacca tgatcctggt cgtctaccgc cacggcctgc gagcctcgga
     2041 ggtctgcgac ctccaatggc agcagatcga actctccgag ggccgcctgc acgttcaccg
     2101 ggtgaagaat ggaatcccca gcgtccaccc aatccgcggt gacgagatgc gagcgctacg
     2161 caagctacgc cgcgattacc ctaaagaggc ttacgtgttc gtttccgaac gcggtgggcc
     2221 gatcagcccc atcggcttcc atcggctcat ccagcgtgtc ggggagaccg ccaggatgcc
     2281 attcccgatc cacccacaca tgcttcgcca tgcctgcggg ttcaagctcg ccaatgatgg
     2341 tcacgatacg cgggccttgc agcactacct cgggcacaag aacattcagc acaccgtccg
     2401 atacaccgaa atggcgcccg accggttcaa aggcttttgg agggactgac gattccgggt
     2461 gctctccccg cacgggcacg ccgctgcggc agatcgtaag atcacgtgtc cagtggcgat
     2521 ggcacacgct tcctcggctt gcactccttc ggcgcaaccg cggtatcgac gtgttcgagc
     2581 agccggttcg aacgcttgtg ctggggctgc tcaagctcgg ctggacccag cattccaacg
     2641 ggttccgcag tgccctcgat gaccgctccg ctcgccgcag ccccgctctc caacatctgc
     2701 ttaacgaggt cgatgcccgc ctctagctct tcatccgtcc agttcttcag cgaattgctg
     2761 tgctcgacct tgtgctcacg agggaccagc agagcaagga ccttgagata ggccgccggc
     2821 tgcgtgcggc gcactttcgc aatcgccttt tgcccgtgct cacgaaaatc gcgcaagagc
     2881 gcgcaaatga cctcctcgct cagcttgtta cgcgagccgc gcagtcgtcc ggcagggttg
     2941 cctgactgcc ctggctccca aaggttggag ggcatgtgtg ctggtaccgt gttgctgttt
     3001 tgcaaatgta tgatggcgtt attgattcgg caatctgccc gaagccaaag gaaggtagat
     3061 cgtctgctgc tcgtagtcag agcgcttcgc aagctacgcc gcgattaccc gagagacgct
     3121 cacgtattcg tttccgagcg cggcgggcca atcagcccca tcggtttcca ccgcctcatc
     3181 cagcgcgtcg gcgaagccgc caagatgcca tttccgatcc acccacacat gcttcgccac
     3241 gcctgtgggt tcaagctcgc a
//
LOCUS       DATLJT010000259         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2311318,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000259 DATLJT010000000
VERSION     DATLJT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311318"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VM910_05865"
     CDS             complement(<1..1646)
                     /locus_tag="VM910_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI62106.1"
                     /translation="MNLRRALAVAALLLGVCIFILRANGYELYIVALVGLTTIVGVGL
                     NVLLGMTGQISLGHAAFYAIGAYVVGILTTKLDWTFWTTLPLAGVIAGVAGVLLAIPA
                     LRVRGPYLAMVTIAFGFVVEQGAAEWEGLTGGWNGVLGIPLPSLFGFDFSEREMAFLV
                     FCFTVLSLYLYARLSASPWGKAMRAVRDSEIASQSIGLDPTLIRATAFAISAITAGFA
                     GAMFASVTNFISPESFPFFQSILFLLVVMIGGADRLLGPLVGALVVVLLPEILSFLAQ
                     YRLLFVGLLLLLVLRLAPAGFVGLATELIKERKPSPTATRRRDVMAYLSAGSTGRSLS
                     ASDLSVNFGGVRAVNNLSFDARPGDITSIIGPNGAGKSTVLNLMCGFYRPDSGIVRLG
                     EADVTRMATHSIAQQGIARTYQTTQLFTNMTVLDNVLIALRRGHLGAGTIFSRGRNLD
                     HQEIAESLLAFVGYRAPLGQLAGALPHVDKRLVELARALAIQPSVLALDEPAAGLDPG
                     DTARIGDLLRKVARTGITVILVEHDMNLVMSVSDRVIVLDA"
     gene            complement(1643..2515)
                     /locus_tag="VM910_05870"
     CDS             complement(1643..2515)
                     /locus_tag="VM910_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62107.1"
                     /translation="MVSSALISGLGLGSMYGLLALGFHITHAVSNTVNFAQGSAMMLG
                     AVLCFTFWVTFHWPLTLAVIMGLALCAVWGTIVERIAVRPFVQRGSNAWLMATVALGI
                     VLENVVLFTFGKDPRGMPAGLLTTNSVVIAGIRIQLLQLLIPVAGLLLAAAIQIFFQR
                     FHYGKALLAVVQNKDAARLMGIDVERVVMASFALSGLLAGVAGIMVAPLFTISSTMGT
                     LFGIKAFAVAILGGIGNAWGVVLAGLIYGIAEALIIALAGSTYSQIATFGLVILALAI
                     RPTGLFGHAEVKKV"
     gene            complement(2588..>3332)
                     /locus_tag="VM910_05875"
     CDS             complement(2588..>3332)
                     /locus_tag="VM910_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62108.1"
                     /translation="FRVSAVDDDVDIKLLKYANEKFKAKKAGLMLINNPWGQSNEKGL
                     IAAAKDNSAIAIAGVSKFENNDVDMVPHLTRLKEAGADAIILVVNAPPGAQMMKSRER
                     MGWMVPVVSHWGISGGRFPELAGPTAGDAHFVQTYSFFGKQSPVGERVLAALKKKYPQ
                     IKGPEDIFSPVGTANAYDAMHLLALAIEQAGSTDANAVRSALENLKATYQGLIKTYTL
                     PFTPENHDALGPNDYIMVRYEGDKIIPVE"
BASE COUNT          770 a          993 c          997 g          572 t
ORIGIN      
        1 gcgtcaagga cgatcacgcg atcggagact gacatcacga gattcatgtc gtgttcgacc
       61 aggatgacgg tgatgccggt gcgcgcgacc ttgcggagga ggtcgccaat gcgcgcggtg
      121 tcgccgggat cgagcccggc cgccggctcg tccagggcca atacgcttgg ttggatggca
      181 agcgcgcgcg ctaactcgac gagccgcttg tccacatgcg gcaaagcgcc tgctagctgt
      241 ccgagcggag cgcgatatcc gacaaaggca agaaggcttt cggcgatctc ctggtggtcc
      301 agattgcgac ctcgggagaa gatggtgccg gccccgagat ggccgcggcg aagcgcgatc
      361 aggacattgt cgagaaccgt catattcgtg aatagctggg tcgtttgata tgtccgcgcg
      421 atgccctgct gtgcgatcga atgagtagcc atccgcgtga cgtctgcttc tcccagccgc
      481 acgataccac tatccgggcg atagaagccg cacatgaggt tgagcaccgt gctcttccca
      541 gctccattcg gtccgatgat gctcgtgatg tcgcctgggc gcgcatcgaa gctgaggttg
      601 ttcaccgcac ggacgccacc gaagttcacg gagaggtcgc tcgcagaaag gctgcgtccg
      661 gtgctgcccg cggacaagta agccatcaca tcccgccgcc gtgtcgccgt tgggctcggc
      721 ttgcgttctt tgatcagctc cgttgccaac ccaacgaagc cagcgggcgc cagccgcagc
      781 accagcagca gcaggagccc gacgaagagc agacgatatt gagcaaggaa agaaaggatc
      841 tccggcagca gcacaacgac cagggctccc accagcgggc cgaggagtcg gtcggcgccg
      901 ccaatcatca ctacgagaag aaacaggatc gactgaaaga acggaaagga ttccgggctg
      961 atgaagttgg tcaccgacgc gaacatggcg ccggcgaatc cggcggtgat cgccgaaatc
     1021 gcgaatgcgg tggctcggat cagcgtcgga tcaagcccaa tcgactgact cgcaatttcg
     1081 gaatcgcgaa ccgcacgcat ggctttgccc catgggctag cgctcaaccg ggcgtataaa
     1141 taaagcgaca aaaccgtgaa gcagaacacg aggaacgcca tctcgcgctc tgagaagtca
     1201 aaaccaaata gactcggcag tggaataccc aaaactccgt tccatccgcc ggtcaggccc
     1261 tcccactcgg cggcgccctg ctccaccacg aagccgaacg cgattgtcac catggcgagg
     1321 tagggtccgc gtacgcgcag cgccgggata gccagcagga ccccggcaac gcccgcaatg
     1381 acccccgcca agggcagcgt ggtccaaaat gtccaatcga gcttggtggt aaggataccg
     1441 accacataag caccaatcgc gtagaaggca gcatggccga gcgagatttg gccggtcatg
     1501 ccgagaagga cgttgagacc cacgccgacg atcgtggtca gccccaccaa cgcaacgata
     1561 tagagttcgt agccgttggc tcggagaatg aatatacaaa cgccaagaag aagcgctgca
     1621 acggctaggg ctcggcggag gttcatactt tcttgacctc ggcgtgaccg aacagcccgg
     1681 tcggcctaat ggcgagtgcc aggatcacga ggccaaatgt ggcgatctga ctatatgtcg
     1741 atcccgccag cgcgatgatg agagcttccg cgatcccata gattagccca gccagcacca
     1801 ccccccacgc attgccaatg ccgccgagga tggcgaccgc gaaggccttg atgccgaaca
     1861 gcgttcccat cgtcgacgat atcgtgaaca gcggagcgac catgatacca gcaacaccgg
     1921 caagaaggcc ggacagcgcg aacgacgcca tgacgacgcg ctcgacgtcg attcccatta
     1981 gacgcgctgc atccttgttc tgcaccaccg caagcagcgc tttgccgtaa tggaagcgtt
     2041 gaaagaagat ctgaatagca gccgccaaca ggagaccggc gaccgggatc aagagctgca
     2101 agagctggat cctgatgccg gcgatcacaa cggaattggt cgtgagtaag ccggcaggca
     2161 tgccgcgcgg atccttgccg aacgtgaaca ggaccacgtt ttcgagcacg atgccaagtg
     2221 cgacggttgc catcaaccaa gcgttcgagc cacgctggac aaaggggcga acggcgatgc
     2281 gctcgacgat cgtgccccaa accgcgcaga gtgcaagccc catgatcacc gcgagtgtga
     2341 gaggccaatg gaaggtgacc cagaaagtga agcacaggac tgcgcccagc atcatggcgc
     2401 tgccctgcgc gaaattcact gtgttcgaca cggcgtgagt aatatgaaac ccgagcgcaa
     2461 gcagcccgta catgctgccg agccccaagc cggaaatgag cgctgacgac accaacatgg
     2521 cgacgccgaa aaccgatatc ccagcaaaaa gaaggggcgg cgctaaggcc gcccgtctcg
     2581 ctagtcgtca ttcaacagga atgatcttgt cgccctcgta gcggaccatg atgtaatcgt
     2641 tcggacccag tgcatcgtgg ttctcaggcg tgaagggcag agtataggtc ttgatcagtc
     2701 cttgatacgt cgccttgaga ttttcgagcg ccgagcgcac cgcgttggca tcggtcgagc
     2761 cggcctgctc gattgccaat gcaagaagat gcatggcatc gtaggcattc gctgtgccga
     2821 cgggggagaa aatgtcctcc ggccccttga tttgcggata ctttttcttc aaggccgcca
     2881 gcacgcgttc gccgaccgga ctttgctttc caaagaagct gtaagtctgc acgaagtggg
     2941 catcgccggc agtcggaccc gcaagctcgg ggaagcgtcc gccggaaatt ccccaatgcg
     3001 agacaacagg caccatccac cccatgcgct cgcgtgactt catcatctgt gcgccaggag
     3061 gagcgttgac cacgaggatg atggcgtcgg caccggcttc tttgagccgc gtcagatgcg
     3121 gaaccatgtc cacatcgttg ttctcgaact tggagacgcc ggcaatcgcg atcgcggaat
     3181 tatccttggc ggcggcgatc aaacctttct cgttcgactg gccccaagga ttattgataa
     3241 gcatgagacc ggcttttttc gctttgaatt tctcgttcgc gtatttgaga agcttgatgt
     3301 cgacgtcatc gtcgactgcc gagacacgaa ag
//
LOCUS       DATLJT010000260         3605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4378858,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000260 DATLJT010000000
VERSION     DATLJT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4378858"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..687
                     /locus_tag="VM910_05880"
     CDS             106..687
                     /locus_tag="VM910_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI62109.1"
                     /translation="MIGAKSGESTRDRIVSAAARLFYAEGIRATSVDAVAHKAGIAKK
                     TFYYHFASKDNLLAACLQAQDQPVLDLYARWFTDTEGEIAEKIEGIFSRFAEVASAAG
                     WRGCGFQRTVAELASTPGHPAVKAGAAHKKRFQTWLAKVLSEAGVKQPEVVAVRILVL
                     LDGASVVMLTHRDTSYVRAAGSLAAELVRNAKR"
     gene            complement(800..1177)
                     /locus_tag="VM910_05885"
     CDS             complement(800..1177)
                     /locus_tag="VM910_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI62110.1"
                     /translation="MNALTDARIGIVKAALQLTPEQAKYWPVVEEAIRNRAMGRQVRL
                     AARQRDQGDGDPIDMVRHRADALAQRSAELKQLADAWQPLAGTLTPEQKQRLRFFAAN
                     ILRLVPRAIEARRAETEDEGDED"
     gene            complement(1313..2392)
                     /locus_tag="VM910_05890"
     CDS             complement(1313..2392)
                     /locus_tag="VM910_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI62111.1"
                     /translation="MMALVADQGLALFCGLNAMPKKSFLAEYSSRITSQKVAHLLASW
                     HAQLAGETILSGQSFNLDFHSVPYFGDHPIVESHYLSKRSRRQPSILTFLAQDADSQV
                     FCYSNADIRKGEEVEEIFRFIKFWTHQHGRPPQHLVFDSKLTTYEQLDRLEQKQITFI
                     TLRRRSPTLIAEINDLPSSAWRTINLDVPNRKYRTPKVYEQKVCLRKCTYRQFFIKDL
                     GHDLPTILLTNDRKSTVPQLILRYAKRMLIENALADAVRFFHIDALSSSVGFKVDFDM
                     ALLVLASGLYRIMARRMRGYDDAQARQIFRDLIDMPADVAITDHEVNVRFHRRAHLPI
                     VLASGLIDKPVPVPWWGGLPLRLAA"
BASE COUNT          784 a          938 c         1129 g          754 t
ORIGIN      
        1 ggatctgtga ggggagagga tccggttgat cctctcctac tcggcgacaa tatgcggacc
       61 attatctagc ttccgaatga tctttatact cactaggcgg tatacatgat tggtgcaaaa
      121 agcggcgaat caacgcgtga ccgcatcgtg tcggctgcag cacgcctgtt ctacgcagag
      181 ggcattcgtg caaccagcgt tgacgctgtt gcccataaag cgggaatcgc gaagaaaaca
      241 ttctactatc atttcgcgag taaggacaat cttctggcgg cctgtctcca ggcgcaggac
      301 caacccgtgc ttgatctgta cgctcgatgg ttcacggaca cggagggtga gattgcggaa
      361 aagatagaag ggattttttc acgatttgct gaggtggcca gcgcggcagg ctggaggggt
      421 tgcggtttcc agcgcacggt tgccgaactc gccagcactc ccgggcatcc ggcagtaaag
      481 gcgggcgcgg ctcacaagaa gcgcttccaa acttggttgg cgaaggtgct ttcggaagca
      541 ggtgtcaaac agccagaagt agttgcggtc aggatacttg ttctgctcga tggtgcctcc
      601 gtcgttatgc tcacccaccg tgatacatcc tatgtgcgtg cggctggtag cctggcggcc
      661 gagctcgtgc ggaatgccaa gagatagagt agaattcgtg cgagtgcagg gagtttctcc
      721 gtggtctcca gttatcgcgc cggacgaaac ctatacttga gaaagggccg cccttgaggg
      781 gcggccctga tagcgcgctt tagtcctcgt cgccctcgtc ctcagtctcc gcgcggcgag
      841 cttctatggc cctgggcacc agacgaagaa tatttgccgc gaaaaagcgc aaccgctgct
      901 tctgttcggg cgtgagggtt ccggcaagcg gctgccatgc gtcggcaagt tgtttaagct
      961 ccgctgaccg ttgagctaat gcgtcggcgc ggtgacgaac catatcgata gggtcaccgt
     1021 caccttgatc gcgttgccgt gcggccagac ggacctggcg ccccatcgcc ctgttgcgga
     1081 tagcctcctc cacgacgggc cagtatttcg cttgctcagg cgtcagttgg agtgccgcct
     1141 tgacgatgcc aattcgtgcg tcggtcagag cattcaagtc agctgcgctt agtcgagcag
     1201 cctgctggtc tcctccagaa gtagcagtag tagtggcgga aggagcctgt gcgtaagcta
     1261 gcctggattt ccacggaacg gaattgagct aacaccggta ggcggggctg gcctatgcgg
     1321 ccaaccgaag cgggaggcca ccccaccaag gtactgggac gggtttgtcg atgaggcccg
     1381 aggcgagaac gataggcaaa tgggcgcgcc gatggaagcg cacgttgact tcgtggtcag
     1441 tgatcgcgac atcggccggc atatcgatca ggtcacggaa gatttggcga gcctgagcat
     1501 cgtcatatcc gcgcatgcgg cgcgccatta tgcgatagag gccgctggcg agcacaagaa
     1561 gggccatgtc gaaatcaacc ttaaagccga cggatgagga gagcgcatcg atgtggaaga
     1621 agcgcacagc gtcggcgagt gcgttctcaa tgagcatgcg ctttgcgtag cgcaggatta
     1681 attgaggcac agtggatttt cggtcattcg tgagcagaat ggtcggcagg tcgtggccga
     1741 gatccttgat gaagaactgg cggtaggtgc atttccgcaa gcacactttc tgttcataga
     1801 cttttggcgt gcggtatttg cgattgggaa cgtcaagatt gatggttcgc caggccgagg
     1861 acggcagatc gttgatttcg gcgatgagcg ttggcgagcg tcggcgtaaa gtaatgaagg
     1921 tgatttgctt ctgctcgagc cgatcgagct gctcataggt ggtgagcttg gagtcgaaga
     1981 ccagatgctg cggcggccga ccgtgctggt gggtccagaa cttgatgaag cggaagatct
     2041 cctcgacctc ctcacccttg cggatatcgg cgtttgagta gcagaagact tggctatcgg
     2101 catcttgggc gaggaaggtg agaatacttg gctgccggcg acttcgcttt gataaatagt
     2161 gggattcgac gatgggatgg tcaccgaagt aaggcactga gtggaagtcg agattaaagg
     2221 actgacccga gaggatcgtc tcgccagcaa gctgagcatg ccaactggca agcagatgtg
     2281 cgactttctg cgaggtgatc cgggaggaat attcggccag aaagctcttt ttgggcatgg
     2341 cgttgagacc gcagaacagc gcgagcccct ggtcagcgac cagcgccatc acgtggctct
     2401 tgcgctcgat agaccatagc ttgagagcca gcgaggcgcg gagcgcgtgt tcggccggga
     2461 tcatgcgaga gccgggcagt ttggcattcc gggcaagtgc ctcacaatcc aatcggatta
     2521 gatcgggcac gaacaggaac aaccctccga ctctggtggt gaattcgcgc ggagagagcg
     2581 caaaggcacg aacatcggca acggcctccg cggtcggccc aatcaagacc gggcgttcct
     2641 catcgagccg gcggggcaac ggagcaaagc cctcggcggc caagatctcc cgaactgcag
     2701 tagcgctcaa tatgattcct tgctccttga gcgctcggct gatgtcgtag atcgagtagt
     2761 tgcgcttgcg cagcgctaac acctgatcgc gggccttgct tttcttaggc tgttcacgcg
     2821 gaccaggccg tgtcgtggcg aagaaatcgc gtagcaggcc gcgtcggaag tcgtggcaaa
     2881 gcacgcgaaa cgctccgtgt gaatagccga accggtgcgc gacctccttg gaagttaggc
     2941 cgtccaggaa aaacgcccgc aaggcttcgt actggcgctg gcgcgcggtc gccggttcgg
     3001 cgaaaaagcg gcggcaatct gttatgtcaa ttggtgcgcc gatacccatc catcgacaat
     3061 gtcatccaca atatcttgtg tcaagcaact cttgcggcac aaaatcaaat aaccgcaaga
     3121 agtcggcgtt tatcaacgat ttctaggcac attcgccgaa actcggaccg ctcatcgact
     3181 tggataaaca gataaagaga gggcgcaaaa atcgattatc agttcaccat tttggataac
     3241 acatagagtc aaattgtcga gtcaaatgaa tcccttagcg cggcgccgcg ttagctgatt
     3301 cgggttccgt gtaaatccag gctagtgaag aggcagtaac gaagagggca gtcaatccgg
     3361 cagcgactgt cttgaacatc agcacctccc ttgattagct attgctcttg atgggcctga
     3421 tcacgagcgc gcgatcgcgg ctaaactttg cacgagaggt aaggcctgtc gatcctcctg
     3481 aaaggctacg attccgcaaa acgatggggg ctttttcgcg ttttagggcc tgcgcgcgtg
     3541 cgctcgctcg acgcggtgca tggccgggtc gctggggcat caaaaggttg cgacatgggg
     3601 cgctc
//
LOCUS       DATLJT010000261         5832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1622116,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000261 DATLJT010000000
VERSION     DATLJT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622116"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..411
                     /locus_tag="VM910_05895"
     CDS             235..411
                     /locus_tag="VM910_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62112.1"
                     /translation="MKMLIAALTLATLISGPAFTQPTAAASNGGRGVNQSGQNYNGYY
                     RGYPLEEWYRTDGW"
     gene            608..1096
                     /locus_tag="VM910_05900"
     CDS             608..1096
                     /locus_tag="VM910_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HVI62113.1"
                     /translation="MIKHFNVSEKGHGSYWLLGILRWVMVLIFVSFGIQKFTPQSADG
                     IALYISNSPFVSWLSIFGIRGEAYLLGIIELTTAALLAAGAFIPILSALGSLMGIGTF
                     AITWSFFFSTPGVVKWSVSTDPIAWNLTGEFLYKDVVLLCVCIVLFLASLPKKIVPLR
                     SN"
     gene            1269..1442
                     /locus_tag="VM910_05905"
     CDS             1269..1442
                     /locus_tag="VM910_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62114.1"
                     /translation="MEINETVLEVIALTRAEIAKNGVSVRTELAETLPAIQGDRVQLQ
                     QVILNLLINGIDR"
     gene            1448..2047
                     /locus_tag="VM910_05910"
                     /pseudo
     CDS             1448..2047
                     /locus_tag="VM910_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2666..3346
                     /locus_tag="VM910_05915"
     CDS             2666..3346
                     /locus_tag="VM910_05915"
                     /inference="COORDINATES: protein motif:HMM:NF023420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HVI62115.1"
                     /translation="MSRLTDTQLIILSAASQRDDRGVELPANIRGEAARKVVDKLIRA
                     GLLEEVPAGGALPVWRRDDDSGAMALRMTKNGLEAIDVEDEAAAAPKEMSVSPAPAHA
                     GETAASKTSASRKRSAVAAQKSARKKHHSGKAKTKASSPRESRPGSKQDRVLAMLRRP
                     EGATIAAIMRATHWQQHSVRGFFAGVVRKRRGLDLRSEKVDGDRVYRIVHSGGARSDS
                     RRSHRRAA"
     gene            3352..3846
                     /locus_tag="VM910_05920"
     CDS             3352..3846
                     /locus_tag="VM910_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HVI62116.1"
                     /translation="MVRVVIDPAVPDRASLDFEIARLRGLDIGELRARWHTVFRRRAP
                     PHLPRHLLFRILAYRLQADRLGELDNDSRRLLDRIGSGSAERIDRLVADFNRPRTEFR
                     PGTLLTREWDGHLQQVMVLADGFAWNGKTYRSLSKAAFAMTGTRWSGPRFFGLDQPPS
                     ERRP"
     gene            3843..5438
                     /locus_tag="VM910_05925"
     CDS             3843..5438
                     /locus_tag="VM910_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI62117.1"
                     /translation="MKSGTVKPVRCAIYTRVSTDHGLEQDFNSLDAQYDAAQAYIRSQ
                     AHAGWTLVRSRYADGGYSGGSTDRPALQRLLADVKAGKVDVIVVYKVDRLTRSLADFA
                     KLVELFDGHGVSFVSVTQQFNTTTSMGRLTLNVLLSFAQFEREVTSERIRDKIAASKR
                     KGLWIGGIVPLGYATKDRKIVVVEEQAERVRTIFRRYLQLGSLNLLMADLRARGIVTK
                     VRSLKSGRTVGGIPFTRGPLAHLLRNRFYVGEVVFKGEVLPGEQLAILDRDLFDAVQT
                     KLTEQRNTHTVARSKSESLLIGRIYDDRGNRMTPSHVRKGGIKYRYYLSSPLLHGQPA
                     RAGSVSRVPAAEVEARVARTVREHFEDRTGSDDRDLIRNHVVRVEVQAHQLAVELKAE
                     KAPSNPRNAVDNDRLVLRIPWKKTPMKRRREVIVPVSVPPHEVRPIRAETRATLVASI
                     ARGRRWLDEIVAGTVTSVEQIAARDKCSIRQVNMTISLAFLAPELVKAAVEGRLPRGI
                     GVTRLRNAPAEWSRQYTMLGLSI"
BASE COUNT         1179 a         1778 c         1726 g         1149 t
ORIGIN      
        1 aggactgcag ctacttcaac gactccgact gggactatgc ctatcgggcc tgtatggctc
       61 aacacggcga agcggagtga tctcgtcgga gcgatgaaca gggccgcgat ctctggcgcg
      121 tggttgcgcg aaaagtgcgc attccgaagg cgacgcacag ctgcaagcgc cggccgattg
      181 gcccccgctg atgcacaatt cgaccgacga gggtccgtaa catggagacg tactatgaaa
      241 atgctcatcg ccgctctgac cttggccacg ctcatttccg gccccgcttt cacccagccc
      301 accgccgcag cgtccaacgg tggccgcggc gtcaatcagt cgggacaaaa ctacaatggt
      361 tactaccgcg ggtaccctct ggaggagtgg tacaggactg acggctggta aagttcaatc
      421 agctgccacg cgaaggtctg aacgtgaatc gatccatgca cgctggcatg ctccgatcca
      481 cacaacatcg aacaagaaga tgtgtcatga agccactgat cgctgctctc gcgttggtta
      541 cactgattgc tgtcccgaga gggttccttt cagcgcacag gtacggcagg cgatggaaga
      601 ggacatgatg attaaacact ttaatgtttc tgaaaaaggt catggttcgt actggctgct
      661 cggcattctc cgatgggtga tggttctcat ctttgtttcg ttcggcatac agaaattcac
      721 gcctcagtca gcagacggca ttgcattgta catttccaac agtccgttcg tttcgtggct
      781 gtctatcttc gggatcagag gcgaggccta tctgctcggc attatagagt tgacgacagc
      841 ggctcttctc gctgccgggg catttatccc gattctgtcg gcactcggct cgttgatggg
      901 aatcggtacg tttgcgatta cgtggtcatt tttcttctcg acacccggtg tcgtcaaatg
      961 gagcgtatcc acagacccga tagcgtggaa tctcaccggg gaattcttgt acaaagatgt
     1021 cgtccttctt tgtgtttgta tcgtgttatt tttggcatca ctgccaaaga aaatcgtgcc
     1081 gcttcggtcg aattgacgtg tcggtgttgg cgcccgaatc tcgcttatct agcagtgctt
     1141 tttgctcggt gcgttagtca catgatcaca ccgcgattgg cttgcctcat cagagatggt
     1201 cggcgggcca gcgatgtcat caatctgatc cgcgcctcgt caaaaaggcc cctccacgga
     1261 aggatcgatt ggagatcaac gaaacagtct tagaagtcat tgcgttgacg cgtgccgaaa
     1321 tagcgaagaa cggcgtttcg gtacggacgg agctagcgga gacactgcca gctattcagg
     1381 gagatcgagt gcaactgcaa caggtgattc tcaatttgtt gatcaacggt attgaccgat
     1441 gagtggaatg agcaggggtt cagccaagcc cattcgttgc gcgatctata ctcgcgtctc
     1501 gaccgaccaa gggctcgagc aggacttcaa ctcgctcgac gcccaatatg atgcctcgca
     1561 agcctatatc cgcagccaag cgcacgccgg atggagcctg cttcgagcca aatacgagga
     1621 cggggggttc tctggcggca acaccgaccg gccggccctg cagcggctcc ttgacgacgt
     1681 gcgcgccggc aagatcgacg tgatcgtggt ctacaaggtc gaccgcctga cccgctcgct
     1741 gacggacttt gcaaaacttg ttgagctgtt cgacacgcat ggtgagtcct tcgtatcggt
     1801 cacccagcag ttcaacacca ccacgtccat gggcagattg acgttgaatg tactgctgtc
     1861 gtttgcccaa tttgaacgtg aggtcacctc agagcgcatc cgcgacaaga tcgccgcctc
     1921 caaacgcaag ggtctctggg tcggcggcat ggcgccgctc ggctacgaca ccaaggatcg
     1981 caagataacc gtcaatgggg ccgaggctga ccgggtcggg accatcttcc gcagctatct
     2041 gcggctccga cctcggctcg taggcgtcga attataggtc ccgcctctcc catagcccgt
     2101 tccggggtac ggatttggcg tgcagaaccc atagatgccc gacacggtcg cgcggcattg
     2161 ctcgaacgag gtgtagctgc agttgatcgc cccatctagg ccggtgccgt aattcgcgca
     2221 ccaactgccg ttagcaccag cacgcgcggc ggtcggggac agggatagag caaagaaggt
     2281 gatcaaaacg agcgggatcg ttcgcatgac tgcctcctcg gccgcaggca ccccccgatc
     2341 cgatcttcac gttagcagat ttgctcgcgt tttcggaaga tactagatct tttacgaaac
     2401 tgggcccgct tttcggaatc gcgtggctcg cctcctatct gcgcatggaa atcggcaccg
     2461 cggggatcat cccatgctcc gatctgcctc aaaccagcaa taacattatc cagaaaggac
     2521 tggcatttgg tcgcgaacag agcggtagtg tgaccgttcc gggcgcgagt agcacctcgg
     2581 gatcctgccc cgccgcctgc atggccaagg tggggctgtg gtggtggcgg caaatgacgc
     2641 cgcaccaacc cagcggagga tcccgatgtc aagacttacc gatacccaac tcatcattct
     2701 gtccgcggct tcgcaacgcg atgatcgcgg ggttgaactg ccggctaaca tcaggggcga
     2761 agccgcccga aaggttgtcg ataagctcat ccgcgccggc ctgctggagg aagtcccagc
     2821 gggtggcgcg ctgccggtct ggcgacgcga tgacgacagc ggagcgatgg cgcttcgcat
     2881 gaccaagaac ggcctcgaag cgatcgatgt cgaggatgaa gcggcagccg ctcccaagga
     2941 gatgagcgtc tcccctgctc ccgcccacgc aggcgaaacg gctgcatcca aaaccagcgc
     3001 ctcccgcaaa cggagtgccg ttgcagcaca gaagtccgct cgcaagaagc atcactcggg
     3061 caaggccaaa accaaagcca gctcgccacg cgaaagccgg ccgggctcga aacaagatcg
     3121 agtgctcgcg atgctgcgcc gaccggaggg agcgacgatt gctgccatca tgcgagcgac
     3181 ccattggcag cagcactcag tccgcggctt cttcgctggg gtggtgcgca agaggcgcgg
     3241 cctcgatctg cgctcggaga aagtcgacgg tgaccgggtc tatcgtatcg tccacagcgg
     3301 cggtgcacgc tccgattccc gccgttcgca tcgtcgcgcc gcttgagcgc catggtgcgc
     3361 gtggtaatcg atccagcggt gccggacaga gcgtcgctcg acttcgagat cgcgcgtctg
     3421 cgcggtctcg atattgggga actccgcgcc agatggcaca cggtgttccg gcggcgagca
     3481 cctcctcacc tgccccgcca tctgctgttt cgtattctgg cctatcggct gcaagccgat
     3541 cgactcggcg aattggataa cgacagtcgg cgtctgctcg accggatcgg gtcggggtct
     3601 gcggaacgga ttgaccgcct cgtggctgac ttcaaccggc ccagaaccga gtttcggccc
     3661 ggcaccctgc tcacgcgcga gtgggacggt cacttgcagc aggtcatggt gctcgccgac
     3721 ggcttcgcct ggaacggcaa gacctaccga agcctgtcta aggccgcctt cgccatgacc
     3781 ggcacccgct ggtctggacc caggtttttc ggcctggatc aaccgccatc ggaacggcgg
     3841 ccgtgaagag cgggaccgtc aagccagtcc gctgcgcgat ctacacccgc gtgtccaccg
     3901 accatgggct tgagcaggac ttcaactcac tcgatgccca atatgacgcc gcgcaggcct
     3961 acattcgcag tcaggcgcat gcaggctgga ccctagtccg gtctcgctac gctgacggtg
     4021 gttactcggg tggctcgacc gaccgccctg ccctgcaacg gctattggcg gacgtgaagg
     4081 cgggaaaggt cgatgtcatc gtcgtttaca aggtcgaccg cctgacccgc tcgctggcgg
     4141 acttcgccaa gctcgtcgag ctgttcgatg gccatggcgt gtccttcgtc tcggtcaccc
     4201 agcagttcaa taccacgacc tcaatggggc ggctgacgct caatgtcctg ttgtcgtttg
     4261 cccagttcga gcgggaggtc acctccgagc gcatccgcga caagatcgcg gcttccaagc
     4321 gcaaggggct gtggatcggg ggcatagtcc cgctcggata tgcgaccaag gaccgcaaga
     4381 tcgttgtggt ggaagagcaa gccgagcggg tccgcactat ctttcggcgg tatttgcagc
     4441 ttggcagcct caatttgttg atggccgacc tccgcgcgcg agggattgtc accaaggtgc
     4501 gatctctcaa gagcgggcgc acggttggcg ggatcccgtt cacccgcggt ccgctcgccc
     4561 atcttctgcg caatcgattc tatgtcggcg aggtcgtctt caagggcgag gtcctccccg
     4621 gcgagcagct cgccatcctc gatcgtgatc tctttgacgc ggtgcaaacc aagctcaccg
     4681 agcaacgcaa cacccacacg gtcgcacgat ccaaatccga atcgctgctg atcggtcgca
     4741 tctatgacga ccgtggcaac cggatgacgc caagccatgt ccgcaaggga gggatcaaat
     4801 atcgatatta cctgtcatca ccactcctcc atggccagcc cgcgcgtgcc ggatctgtga
     4861 gccgggtgcc ggcggccgaa gtcgaagcgc gcgtcgcccg caccgtccgc gagcatttcg
     4921 aggatcgcac aggaagtgat gaccgagacc tcatccgcaa ccacgtcgtc cgagtcgagg
     4981 tgcaagcgca ccagctggcg gtcgagctga aggcagagaa ggcacccagc aacccgcgca
     5041 acgcggtgga caatgatcga ctcgttctgc gcatcccatg gaaaaagacg ccgatgaagc
     5101 gacggcgcga ggtcatcgtg ccagtatcgg tgcctccaca cgaggttcga cccattcgcg
     5161 ccgagacccg cgccacgctc gtcgcatcaa tcgcgcgtgg ccgacgttgg cttgatgaga
     5221 tcgttgccgg caccgtcacc agtgtcgagc aaatcgctgc gcgggacaaa tgcagcattc
     5281 gacaagtcaa tatgacgatc tcactcgcgt tcctcgcgcc cgaactcgtg aaggctgccg
     5341 tcgaaggacg actgcctcgc ggcatcggcg tcactcgcct gcgcaatgct cctgccgagt
     5401 ggtctcgcca atacacgatg ctcggtttgt cgatttgatc ccggccgatc atcgcggttc
     5461 ttcacgtgca actctggcct cggcgccagg aacgggtttg gagagcccag agaccggggg
     5521 caaaatcgac gctatcgcgg cctcgaccag cagcagagac cgcactcccc ccatttgaac
     5581 ccgcgaaaat gcccgcaaag tgcgcactat tcgtcagaga ccgggaaacg ccggttcgca
     5641 tcggactgcg tggtggtgga tgcactccag atcgaaccgg tctccagctc aaaattccct
     5701 gctataccgg ctctacgcga cgacccgaaa ctcgtcgggc gagatgatac gaaagttgtc
     5761 ggtgattcgg tcgcgaaagc ggtcccattt tttggtaaca gttcgcctga ggaagcgcag
     5821 gattgccgat ct
//
LOCUS       DATLJT010000262         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_13844,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000262 DATLJT010000000
VERSION     DATLJT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13844"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VM910_05930"
     CDS             <1..328
                     /locus_tag="VM910_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62118.1"
                     /translation="PESAPPEKPTIIRRSDLRIERIDRLGLELGTHDDSKLDVAEDDP
                     LSAVNDLRRTWTVSRDAWQVRIETRMRLSCTRDVFLLQASLRAWEGDDEVCRRDWDRS
                     IARDFL"
     gene            complement(399..1064)
                     /locus_tag="VM910_05935"
     CDS             complement(399..1064)
                     /locus_tag="VM910_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62119.1"
                     /translation="MKPAQMRITPQAGADIRAAPGSGGAAHRLPPDHQGICVILCGVA
                     LLLSADLVSATDIDATSFRCITEMTPVRQFYVDNLQGNLDATLAATHSKAGALYPPGS
                     VIQLIPGEAMVKRDRGFNAATHDWEFFELDVSKDGTKIRKRGTTDVVNRFGGNCFDCH
                     VAAAARWDLVCETDHGCAPLPVTRAMIRALQRTDPRCENPSTSPEDAELLKQLEQLLK
                     PPR"
     gene            complement(1071..2081)
                     /locus_tag="VM910_05940"
     CDS             complement(1071..2081)
                     /locus_tag="VM910_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HVI62120.1"
                     /translation="MSNHFTGLSLGPPLGDQRLDLCDLYAFQSPADKNRTVIILNANP
                     NADALHPDAIYRLNIDTDGDYLTDIAFSYVFSKPQNGRQTVSVFAATGSQSRSAEAVG
                     TKIIADAEVSFGAKPNIIKSGPYTFFAGSRSDAFFFDFDGIKNLFDIRGKRNFTEPHL
                     GGKSPWTGVDSNTQANVFSTAIELPTSELVSKPEVHIWGRCSVRRDGKLLHVDRAGHP
                     SVSSFFNTDDTKEEYNASEPVNDRKRWLDQFVHLMGHTGNYTREEAIAAIDKEGALPD
                     VLSFDPSKPAKYPNGRIFTDDVINYRIAFLTKNQAPPTGLKPHTDTLKEFPYLGTPHA
                     KK"
BASE COUNT          384 a          621 c          677 g          469 t
ORIGIN      
        1 tccggaatcg gcgccccccg agaagccgac gatcatccgt cgcagcgatt tgcggatcga
       61 gcgtatcgac cgcctcggcc tcgaattggg cacgcacgat gactcgaagc ttgacgtcgc
      121 ggaggacgac ccgctcagcg ccgtcaacga cctgcggcgg acctggacgg tgtcgcggga
      181 cgcatggcaa gtccgcatcg agacgcggat gcggctgtcg tgcacccgcg atgtcttcct
      241 gctgcaggca agcttgcgcg cgtgggaagg cgacgacgaa gtctgtcgtc gcgactggga
      301 tcgctcgatt gctcgcgact tcttatgaaa atgtaagagc caacgacgat gcggcggtca
      361 gccgtcccgc gttaccgcgt ggtggttcgc cgcgcatttc acctcggtgg cttgagcagt
      421 tgctcaagtt gcttgagcag ttcggcgtcc tcagggctgg tgctcgggtt ctcgcaacgg
      481 ggatccgttc tttgcaaagc gcgaatcatc gcgcgtgtaa ccgggagcgg cgcgcaacca
      541 tgatcggttt cgcacacaag atcccatcgt gcggcagctg caacgtgaca gtcaaagcag
      601 ttcccgccaa accgattgac cacgtcggtc gtccctcgtt tgcgaatctt tgttccgtct
      661 ttcgagacgt cgagctcgaa gaactcccaa tcatgcgttg cagcgttgaa gcccctgtcg
      721 cgcttgacca tggcctcgcc cggaatcaat tgaatgacgg atcccggtgg gtacagggca
      781 cctgccttcg agtgggtggc ggccagcgtc gcgtcgagat tgccttgcag gttgtccacg
      841 tagaattgac ggaccggggt catctccgtg atacaccgga atgaggtcgc gtcgatatcc
      901 gttgcgctca cgaggtccgc cgaaagcagc aatgcgacgc cgcacaagat gacacaaata
      961 ccttggtggt cgggtgggag gcggtgcgcc gcgcctccac tacccggggc tgcgcgaatg
     1021 tcagcgccag cttgcggtgt gatccgcatc tgtgccggtt tcatgcgccg ctactttttc
     1081 gcatgcggtg tgcccaagta cggaaactcc ttcagggtgt cggtgtgggg cttgaggccc
     1141 gtgggcggag cctggttttt cgtcaggaag gcgatgcggt aattgatcac gtcgtcggtg
     1201 aagatccggc cattgggata ctttgcgggc ttcgacggat cgaaactcag cacgtcggga
     1261 agcgcgccct ccttatcgat cgcggcaatc gcttcctcgc gcgtgtaatt gccggtatgc
     1321 cccatcagat gcacgaactg gtctagccat cgcttgcggt cgttgacggg ctcgctcgcg
     1381 ttgtattcct ccttggtgtc gtcggtgttg aagaagctgc taacgctcgg atggcccgcg
     1441 cgatcgacgt gaagcaactt gccgtcgcgc cttacactgc accgccccca gatgtggacc
     1501 tcgggcttgg aaacgagttc actcgtcggc agctcgatcg ccgtcgaaaa cacgttcgct
     1561 tgcgtattcg aatccacgcc ggtccatgga gacttgccgc caagatgcgg ctcggtgaag
     1621 ttccgtttac ccctgatatc gaacaggttc ttgattccgt cgaagtcgaa aaagaacgcg
     1681 tcgctgcggc tgccggcgaa aaaagtgtag gggcccgact tgatgatatt cggcttggcg
     1741 ccgaaggaaa cttcggcgtc ggcgatgatc ttcgtcccaa ccgcttcggc cgagcgcgac
     1801 tgcgagcccg tcgccgcgaa tacgctgacg gtctgcctgc cgttctgcgg cttcgagaac
     1861 acatagctga acgcgatgtc ggtcaggtaa tcgccgtcgg tatcgatgtt gaggcgatag
     1921 attgcgtcgg gatgtagtgc gtccgcgttg gggtttgcgt tgaggatgat cacggttctg
     1981 ttcttgtcgg cgggcgactg gaatgcatag aggtcgcaga ggtctaaccg ttgatcgccc
     2041 agcggcgggc cgagactgag tccagtgaaa tggtttgaca ttggcctttc ctgttgatcg
     2101 agttttcctt tcggctcgct cggacggcat catacatcaa accgcctctg c
//
LOCUS       DATLJT010000263         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2081797,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000263 DATLJT010000000
VERSION     DATLJT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2081797"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VM910_05945"
     CDS             <1..140
                     /locus_tag="VM910_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HVI62121.1"
                     /translation="CASEQCHWIFFDRSKPANRHWCSSNVCGNRQKTRDYRRRRTLGC
                     T"
     gene            76..648
                     /locus_tag="VM910_05950"
     CDS             76..648
                     /locus_tag="VM910_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HVI62122.1"
                     /translation="MYVAIVRRRGIIDVAGLLVAPELRAPSSALLCGGHCIVSRRHAV
                     QQTASGASATWGGGWNSGEQASFFRIFSEQISQGCVGGHERGNIAAARRLTAASRDFP
                     LTLTVAGEGTVTLAAAPGSNALGRVVAELYGLAKTDRLARLKACASEQCHWIFFDRSK
                     PANRHWCSSYVCGNRQKTRDYRRRRTLGCT"
     gene            836..1000
                     /locus_tag="VM910_05955"
     CDS             836..1000
                     /locus_tag="VM910_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62123.1"
                     /translation="MKKFIPPDTCLDDCKMRRSSFLKELIASTPNVNLRAREFRRWID
                     ENSPYTDPEA"
     gene            1022..1225
                     /locus_tag="VM910_05960"
     CDS             1022..1225
                     /locus_tag="VM910_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62124.1"
                     /translation="MSLADDYFVVIKRRRRLREWGWEIQRRSKPLGVRLDGDGFKSEF
                     AAKLAGEKVLREFLDDLAQEDNV"
     gene            1849..2025
                     /locus_tag="VM910_05965"
     CDS             1849..2025
                     /locus_tag="VM910_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62125.1"
                     /translation="MVHKSFRCLPAGMTTQIKPASTGVDLILKIGTALLISFAATLVL
                     VDAEVYFAQSLMVF"
     gene            2481..>2794
                     /locus_tag="VM910_05970"
     CDS             2481..>2794
                     /locus_tag="VM910_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62126.1"
                     /translation="MLDKGRRAFIRLLGGAAATWPLAASAKQPEKLPTIGFLGTNTPS
                     AQSRWTAAFVQRLPELGWIEGRTIAIEYRWAEGRIERFADLATELVRLKVDVIVTTGA
                     AV"
BASE COUNT          584 a          781 c          860 g          569 t
ORIGIN      
        1 catgcgcatc agaacagtgt cattggattt tcttcgatcg ctcgaagccg gccaatcggc
       61 attggtgctc ttccaatgta tgtggcaatc gtcagaagac gcgggattat cgacgtcgcc
      121 ggactcttgg ttgcacctga gctgagagca ccatcatcgg cgctgctttg tggtggccat
      181 tgcatcgtat ctcggcgtca tgctgtccag caaactgcat caggagcatc cgcgacctgg
      241 ggtggcgggt ggaactcggg tgagcaagct tccttcttta ggattttctc cgagcaaata
      301 tctcaaggct gcgttggggg gcacgaacgg ggaaacatcg ccgcggcgcg ccggctgacc
      361 gcggccagca gggattttcc gctgactctg accgttgccg gagagggaac cgtcacgctg
      421 gctgcggccc ctggatcaaa tgcgctcggt cgtgtcgtcg cggaactata cggcctggcg
      481 aagacggatc ggcttgcgcg gttaaaggca tgcgcatcag aacagtgtca ttggattttc
      541 ttcgatcgct cgaagccggc caatcggcat tggtgctctt cctatgtatg tggcaatcgt
      601 cagaagacgc gggattatcg acgtcgccgg actcttggtt gcacctgagt gatggcggga
      661 tgtctgctta tggcccaaag cggacatggg cggcctcgcc atcccgcagt gcagccgcct
      721 acggcgcatt acacagctct ggcttagaca aacaaatgtt tggcacgggg taacaagtcc
      781 gcgaacggaa gcaaagcgac taagaagcgc acgaagcaca agaaggcgtg cgaccatgaa
      841 gaagttcatt ccgcccgaca cctgtcttga cgattgcaag atgcgacgta gctccttcct
      901 caaggagctg atcgcatcaa cgccgaacgt gaacttgcgc gcgcgagagt ttcggcgatg
      961 gatcgacgaa aactcacctt acaccgatcc tgaggcctaa atcatcgcgc tgctcggtac
     1021 catgtctctt gcagacgact acttcgtggt tatcaagcgg cgaagacgcc tgcgcgaatg
     1081 ggggtgggaa attcagcgac gatcaaaacc gcttggggtc agacttgacg gcgacggctt
     1141 taaatcggag ttcgcagcaa aacttgccgg tgaaaaagtc ctaagagagt ttctggatga
     1201 ccttgcccaa gaggacaatg tctgagcgcc gtaaagctga caagaaatac cgttaggaca
     1261 ctcacgccgc cagacgttga cgcgacccgc acgtcccatc ggttgcctta tagtcaggct
     1321 ggcgttgcca acaaaaccag cggtttgcac aaactattgc attgtgtagg gacgcgggag
     1381 atagcacgct gaaagcatgc tggaatcaac cgcatccttc cccgccatat tctttggggc
     1441 gatcatcggc gtcatcgttt tcgtcgcctt gagtcaactg ttgctcgatc cgcgccagtg
     1501 acgccgccgc gacagcctag ccaccctccg cccctcggct aaggagaggg cgcatcatga
     1561 gcgcgagggt aaagggggag aggcaggatt ggacttcgga catcgtgtgg gtcgcgctct
     1621 attcgatcct agttctcctg ctcatgcttg catgggcaga cattccggcg tgaagccacc
     1681 ggcgtgccaa cgatcacgcc ggggcggcta agatggcaag ggtcgggcac tgactgtctc
     1741 gcgactactt ccgcggccgc gaatgttgaa acaatgtgag gcgccgcaca ctcggggcat
     1801 cggtcaagga gcgataatcg gcacgttctc gaaccgacgg aggctcctat ggtgcacaag
     1861 tccttccgat gcctccctgc gggcatgacg acgcagatta agccagcatc aacaggcgtc
     1921 gacttgattt tgaaaatcgg cacagcgctc ttgatcagct ttgctgcaac gctcgttctg
     1981 gtcgacgccg aagtctattt cgctcagtcg ctgatggttt tctgatctca aatcgtctgg
     2041 gcggatgcta atgtatgtcc gctgtgaggg gcaaagcgga cctagcccat gtacggcgtg
     2101 aggtctgctt ctgacccaaa gcggacaaag gccggattga aatcccgcag agcggcaggc
     2161 tccccgacgc cctggggccg gctgggccgc gaacactcag ctagattcag aataattcag
     2221 gtttgcccca aagacctgcc actccgttcg gtggcaggct gatggtgcgg agatccatgc
     2281 gtgcgatttg cgacccaagg agatccacca tgaaccagca ggcggtagag gagcggcggg
     2341 attggggtgc cgacgtcatg gaggttgggc tttattcggt tctgatcctc gtggtgacgc
     2401 tgctgtgggt atacattcca gcctaagcgc cttcgcgctt gcgcggcgac cggctagggc
     2461 gcccacggga gggaagcgcc atgctcgaca aggggcggcg cgcgttcatt aggttactcg
     2521 gcggcgcggc ggcgacgtgg ccgctcgcag caagcgcgaa gcagccggag aagctgccga
     2581 ccatcggttt cttgggcacg aatacacctt cggcccagag ccgatggacc gccgcttttg
     2641 tgcagcgact gcccgaactc ggctggatcg agggtcggac gatcgcgatc gagtatcgct
     2701 gggcggaagg acgtatcgaa cgcttcgccg acctcgcaac cgagttggtc cgcttgaaag
     2761 tcgatgtcat tgtcacaacg ggagccgcgg tcag
//
LOCUS       DATLJT010000264         1704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4149819,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000264 DATLJT010000000
VERSION     DATLJT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149819"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..916)
                     /gene="istB"
                     /locus_tag="VM910_05975"
     CDS             complement(77..916)
                     /gene="istB"
                     /locus_tag="VM910_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI62127.1"
                     /translation="MGELKLYGMKAAFDEIMATAVKRQHEPQRIVGDLLNAEINEKHA
                     RSIKYQLSIAKLPLAKDLEDFQFEGTPINQTLVNDLAGGGFIAQQRNAVLVGGTGTGK
                     THLAIAIARSCIRSGARGRFYNVVDLVNRLETEARNARQGRLADHLTRMDFVILDELG
                     YLPFAQSGGQLLFHLVSRLYERTTIIVTTNLAFGEWPSVFSDAKMTTALLDRLTHHCD
                     IIETGNESWRFKSRADDQIPTRARVVSATPTSSDDASATTRARRLRGSKLDADRGSNL
                     EAD"
     gene            complement(819..1704)
                     /locus_tag="VM910_05980"
                     /pseudo
     CDS             complement(819..1704)
                     /locus_tag="VM910_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200010.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          305 a          481 c          581 g          337 t
ORIGIN      
        1 cggatcgacc atgaaatatt tgtgcgtgtc gagcagggtt cgctgtgttc tgctcgaagg
       61 aatgcgtccc atgttgtcaa tcggcttcca aatttgaccc acgatcggcg tccaattttg
      121 accctcttaa gcgacgagct cgggtggtag cgctcgcgtc gtcggagctg gtcggggttg
      181 cggagacgac gcgagcgcgg gttgggattt gatcgtcggc tcggcttttg aagcgccagc
      241 tctcatttcc agtttcgatg atgtcgcagt ggtgggtcag gcgatcgagc agcgcggtcg
      301 tcatcttggc atcactgaag acgctcggcc attcaccgaa ggcgaggttt gtggtgacga
      361 tgattgtggt gcgctcgtag agccggctga caaggtggaa caagagctgg ccaccggatt
      421 gggcgaaggg cagatagccg agttcgtcaa ggatgacaaa gtccatccgg gtcagatgat
      481 cggcgagccg accctgtcgt gcgttgcggg cctcggtctc gagccggttg acgagatcga
      541 ccacattgta gaaacggccg cgggcgccgg agcgaatgca gcttctcgca atggcaatgg
      601 ccaagtgggt cttgccagtg ccagtcccac cgaccagcac cgcgttgcgc tgctgggcga
      661 tgaagccgcc gccggcgaga tcattgacga gcgtttggtt gatcggcgtg ccctcgaact
      721 ggaagtcttc gaggtctttg gcgagcggta gcttggcgat gctgagctgg tacttgattg
      781 aacgcgcatg cttctcgttg atctcggcgt tgagcagatc accaacgatg cgctgaggtt
      841 cgtgctggcg tttgacggct gtggccatga tctcgtcgaa agcggccttc atgccgtaga
      901 gcttgagttc gcccatgagg tcgaagagtt gagtgcgttc catcagatgg tcctcctgag
      961 gttgtcgtaa cgggcacaat cggcgatggg cgtgtggcgc agcgtcagcg cggccggcgt
     1021 gaggatcgtg gccggcggac ccggatcgcg ttggcgggcg agaatgttga ggacgacatc
     1081 ggcggaatgg acgccgtggg cgatcgcttc ggcgcaggca gcctcgacgg ctggtagccc
     1141 gtcgctgagc accacggtga ggatgtcgac catctgccga ttgccgtcgt cgacgccggc
     1201 gagcttgcgc cgcacgcgct ccatcgcggc cggcagcacc cagtccttga agggtgcgcc
     1261 gttgcgcagg gcaccgggct tacgggccag caccggcacg taatgccagg ggtcataaac
     1321 cgtgtcgcca cggccgaatg agcgaggatg ctcggcgacg atccgcccgt cctggcggat
     1381 cacaatgcgg tcagcatagg cgtgaacctc gaccggccgt ccgaccgcgc tcgcggccac
     1441 cgagtacttg ttgttgtcga agcgcaccag gcaggtcttg gagaccgacg ccggcaccgc
     1501 atggaagcca tcgaaccggc ccgcataggg aacgagcctc ggccgctccg cctcgaacac
     1561 ctcccagatc gtgcggtctg taaactccgg atgacgatgg gccttggcat aggcgatgca
     1621 tttatcgagc agccaggcgt tcagctcgtc atagtttttg aaccgcaggc gcggtgtgaa
     1681 gaaccgctcg cggacgaacc ctac
//
LOCUS       DATLJT010000265         3547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_243598,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000265 DATLJT010000000
VERSION     DATLJT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243598"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VM910_05985"
     CDS             <1..847
                     /locus_tag="VM910_05985"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI62128.1"
                     /translation="GQFTAVAAVRGARFALLEAALAAAERLRSGGRLVYVGAGTSGRL
                     AVQDGAELVPTFSWPQERLLLLIAGGRNALLRSVEGAEDAVDQAARLTQQHEIGPADV
                     VIAVAASGTTPFTLSCLTEAKRRGALTIGVANNRDTPILKEADHAILLDTGAEPIAGS
                     TRMKAGTAQRITLNVFSSVVMILLGRVYDGLMVDLQAVSQKLVRRSESILTRLTGSSG
                     EQAREALHRANGNVKLAVLLLHGCDVKEATDVLHRAGGQLRAALADIGKPGPEPADLS
                     DLPTS"
     gene            complement(1019..1228)
                     /locus_tag="VM910_05990"
     CDS             complement(1019..1228)
                     /locus_tag="VM910_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62129.1"
                     /translation="MAQVITRCRLTGHYMFMGMDVDPKEFTHSPGPFTRKFCPFCACD
                     HLWHKEDSKFLAPKAAPRSHIQQAS"
     gene            complement(1332..1994)
                     /locus_tag="VM910_05995"
     CDS             complement(1332..1994)
                     /locus_tag="VM910_05995"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI62130.1"
                     /translation="MALFMVLAIAGGAAAQTAVDLQLVLAVDASGSVDQVRFELQKRG
                     YVAAFRHARVLQAIRSGPNRAIGVTMVQWTGPVLQVQVVGWTLVADEETAAAFADAIE
                     RAPRQLFGGGTSISGAIDYATTLFPNSPIRANRRVIDVSGDGSNNRGRPVTLARDEAV
                     ASGIGINGLPILALEPDLDRYYYDSVIGGPGAFVVAAQNYETFADAILKKLIIEIAAN
                     DR"
     gene            2199..2549
                     /locus_tag="VM910_06000"
     CDS             2199..2549
                     /locus_tag="VM910_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62131.1"
                     /translation="MKRWTLKAMMIVGCADGRERVPTSAGERRANVGDTAQAGNRDKF
                     RPKPTGNADGGEPFRGSWRWGKYSSSSSLNCEMMRYAFRSAISHPTAAADDGCVGGVA
                     GRAVRLCGLAELPP"
     gene            2479..>3547
                     /locus_tag="VM910_06005"
     CDS             2479..>3547
                     /locus_tag="VM910_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HVI62132.1"
                     /translation="MMVASVVLPVVLFAFAAWLNYRHEYAVADDRIERSLDILHEHTL
                     KVFQTVERAIAEVDEIVRGMADDDIRRDQSRLHERLKRIVDALPEVRAIFLIDRDGRP
                     LVSSRLVQIPADFDSRERSFFAAQTAGDAGTYVSEVLTPRLTGLSTPFFVLSRRRPSA
                     DGTFNGVVAVAVLPQYFEEFYALIGRSPGSLYALIRADGRFLARYPERPDQGLQPGSA
                     LHTTIAQGRERSMQTIRRSQVDAVERRVGHRKLEGFPVYVVAGIDGSAIRGEWVATMA
                     SHLVFGLPATLFLLLIIGVALRRTRRLYAEAERREAAEAALRQAQRLEAIGQLTGGVA
                     HDFNNLLMVVSGSVQRLRRDLT"
BASE COUNT          619 a         1109 c         1171 g          648 t
ORIGIN      
        1 gggccaattc accgcggtgg ccgcggtgag aggcgcccgc ttcgcacttt tggaggccgc
       61 gcttgccgct gcggaaagat tacgcagcgg cggccgtctg gtctacgtcg gcgccgggac
      121 ctctggccgg ctggccgtcc aagacggggc ggagctcgtt ccgaccttca gctggccgca
      181 agagcggctg ctccttctca tagccggcgg taggaacgcc ttgctgcggt cggtggaagg
      241 cgccgaggac gcggtcgatc aagctgcccg gcttacccaa caacacgaaa tcggccccgc
      301 cgacgttgtc atcgcggtcg ctgcaagcgg taccacgccg ttcaccttgt cctgcctcac
      361 cgaggcaaag cgacggggtg ctttgacgat tggagtggca aacaaccgcg atacaccgat
      421 cctgaaagag gccgatcacg cgatcttgct ggataccggc gccgagccga tcgcagggtc
      481 cacgaggatg aaagcaggaa cggcgcaacg tatcaccctc aacgtcttct catctgtggt
      541 catgatcttg ctggggcgcg tctacgatgg cttgatggtg gacctccagg ccgttagtca
      601 gaagctcgtt cgacgcagcg aaagcatcct cacgcggctc actgggagca gcggcgagca
      661 ggcgcgcgag gcgttgcatc gcgccaacgg caacgtaaag ctcgccgtgc tgcttctgca
      721 cggctgtgac gtgaaggagg caaccgacgt tctccatcgc gcgggcgggc agcttcgtgc
      781 ggcactcgcc gacatcggca agcctggtcc cgaacccgct gatttgagcg atctgccgac
      841 gtcgtgatgc gagcagggcc tgaccccccg cggggctcgg ccaacaaaca cagctttgcg
      901 gttcagagaa tggggcgggc ccgctcggct ggcggttagt gatgaatcaa ggtcgcgaac
      961 gcaattccct cactgtggct cccgcttcgc ctttgagagc gacgaacccc ggtagcgatc
     1021 agctcgcttg ttgaatatgg gaccgcggtg cggccttggg cgccaagaac ttcgagtcct
     1081 ccttgtgcca caagtgatcg caggcgcaga acgggcagaa tttgcgtgta aagggacccg
     1141 gcgagtgcgt gaattccttg ggatcaacgt ccatgcccat gaacatgtaa tgaccggtga
     1201 gccggcagcg cgtgatgacc tgagccatcg cacttctccg tcctaggtaa tcaagccgct
     1261 gagacaacat cgccggtcgg cgaaagttcc gagcggcgcg aggctttgcg gccgtgaccg
     1321 gacctcgccg gctagcggtc attcgcggcg atctcgatga tgagcttctt caggatggcg
     1381 tcggcgaacg tttcgtaatt ctgcgcggcg acgacgaagg cgccgggacc gccgatcacg
     1441 ctgtcgtagt aatagcggtc gaggtcgggt tcgagcgcga ggatcggcaa gccgttgatg
     1501 ccgatgccgg atgccaccgc ctcgtcgcgg gcgagcgtca ccggccgccc gcgattgttg
     1561 gagccgtcgc cggagacgtc gatgacgcgc cggttcgcgc gaatcgggct gttgggaaac
     1621 agcgtcgtcg cgtagtcgat ggcgccgctg atcgaagtgc cgccgccgaa cagttggcgc
     1681 ggcgcacgct cgatcgcgtc ggcaaacgcg gcggcggttt cctcgtcagc gaccagcgtc
     1741 catcccacca cctgaacctg cagcaccggt cccgtccatt gcaccatggt cacgccgatc
     1801 gcccggttcg gtccggagcg gatggcttgc agtacgcgcg catgccggaa ggcagcgacg
     1861 tagccgcgct tttgcagttc gaagcggacc tgatcgacgc tgccggacgc atcgaccgcg
     1921 agcacgagct gcagatcgac ggcagtttgc gccgccgccc ctccggcgat agcaagcacc
     1981 atgaacagcg ccaacccgat ggcgcgccgg atcccggcgc ccccgagatg ggcgtgaagg
     2041 cggctgttca ttgtcgtcgg ttggacatga cttgtccggt cgtggcgaga tgcacgacca
     2101 tcacggtaac ttgatcgatt ttcagacgca attttcaaat gtcgtagagg cgacggaggt
     2161 cgggacccgg cggcagccgc gatcaaactg aagttgccat gaagcgctgg acgctcaagg
     2221 ccatgatgat cgtcggctgc gccgacggtc gcgagcgcgt tcccacatct gccggcgaga
     2281 ggagagccaa tgtcggtgac actgcgcaag cgggcaatcg ggacaaattt aggccaaaac
     2341 caaccggtaa tgccgatggc ggcgagccgt tccgaggctc ctggcgctgg ggcaaatatt
     2401 catcctcgtc gtctttgaat tgtgaaatga tgcgctatgc gttccgctcg gcaatcagcc
     2461 atccgactgc tgcagctgat gatggttgcg tcggtggtgt tgccggtcgt gctgttcgcc
     2521 tttgcggcct ggctgaacta ccgccatgaa tatgccgtcg ccgatgaccg gatcgagcgt
     2581 tcgctcgata ttcttcacga gcataccctc aaggtctttc agacggtcga gcgcgccatc
     2641 gccgaagtcg acgaaatcgt gcgcggcatg gcggacgacg acatccggag agaccagtcg
     2701 cgccttcacg agcgcttgaa gcgcatcgtc gacgccctgc cggaagtgcg ggcgatcttt
     2761 ctcatcgatc gggacggccg gccgctcgtg tccagtcggc tcgtgcaaat accggccgat
     2821 tttgactcgc gcgagcgaag cttcttcgcc gcgcagacgg ccggcgatgc cggcacctat
     2881 gtcagcgagg tcctgacgcc gcggttgaca ggtctcagta cgcctttctt cgttctctcc
     2941 cggcgccgcc cgtcagcgga cggtacgttc aatggcgtgg tcgccgtcgc cgtgctgccg
     3001 cagtatttcg aggagttcta tgcgctcatc ggccgcagcc cgggaagcct ttacgcgctg
     3061 atccgtgcgg atggcagatt tctcgcacgt tatccggagc ggcccgatca gggtctccag
     3121 cccggcagcg ctctgcacac gacgatcgcg cagggacgcg agcgcagcat gcagacgatc
     3181 cggcggtccc aggtcgacgc ggtcgaacgg cgagtcggtc atcgcaagct cgagggcttc
     3241 ccggtctacg tcgttgccgg catcgacggc tcggcgatcc ggggcgaatg ggtcgcgacc
     3301 atggcgagcc atctggtctt cggcttgccc gcgacgctgt ttcttttgct catcatcggg
     3361 gttgcgctgc ggcgcacgcg gcggctctac gccgaagccg agcgccgtga ggctgcggag
     3421 gccgcgttgc ggcaagccca gcgtctggag gcgattggac aattgaccgg cggcgtcgcc
     3481 cacgacttca acaatctcct gatggtcgtg agcggcagcg tgcagcggct gcggcgcgat
     3541 ctgacct
//
LOCUS       DATLJT010000266         9491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3460396,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000266 DATLJT010000000
VERSION     DATLJT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3460396"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VM910_06010"
     CDS             complement(<1..873)
                     /locus_tag="VM910_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVI62133.1"
                     /translation="MTARLPSLNGLRAFEAAARHLSFTQAASELNVTQTAISHQIRRL
                     EEELGVRLFIRKNRALALTPKARDYLPGVRAAFNDLRLATDRLLRKDDDHVLTVSTLA
                     SLAVKWLLPRLSTFQEAHPKIDVRITTSANLVDFKSGDVDAAIRYGRGHWPGLRADWL
                     MADELFPVCSPQLLNGKRPLRTPEDLKDHVLLHTTGGNNDDWRLWLTAAGLPTDISKQ
                     PGITFDLSLMTVQAAIDGIGVAVGRTSYVQDDVAKGRLVVPFKITLPADAGFYLVSPE
                     TADPPKLKAFREWLL"
     gene            989..1192
                     /locus_tag="VM910_06015"
     CDS             989..1192
                     /locus_tag="VM910_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HVI62134.1"
                     /translation="MSTYTHQTMTNHHGLGILAHLSETIHTWRQRYETRRELTQWTDR
                     DLHDVGLSWSDVAREAEKPFWRA"
     gene            1265..1885
                     /locus_tag="VM910_06020"
     CDS             1265..1885
                     /locus_tag="VM910_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI62135.1"
                     /translation="MTRLRFDDLKQYSDVLRSRRGDPITVRFVEPRDAEELQNYFRSL
                     SSRSRYNRFLGAMSELPKPVLDNFTHVSERDGFTVIATIQVDGFETIVGEARYIFNPA
                     DGSFEFGLSIEDRWQGSGIGSALLKNLECRAAAFGAERLFGDTLRSNQSMIALARKSG
                     FAFTKNPDDWKLVRFTKEIAVAPEEIPCASWRIAAQNAALTPVVAG"
     gene            complement(1935..2636)
                     /locus_tag="VM910_06025"
     CDS             complement(1935..2636)
                     /locus_tag="VM910_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62136.1"
                     /translation="METPTYKTALIVGVGEGLSASLARLFAREKINVALAARKIEKLG
                     ALCRETGARAYACDAADADQVERLFGLVEREIGAPDLVVYNAGARARGAFVDLVPAEV
                     EQALLISAFGGFLVAQQAARRMLPNKHGAILFTGASASVKGYAQSSPFAMGKFALRGL
                     AQSLARELSPQGIHIGHFVIDGGIRSAARQDPPDRPDSWLDPDAIALSYWNVLQQPRS
                     AWTWEIELRPWVEKF"
     gene            complement(2669..3172)
                     /locus_tag="VM910_06030"
     CDS             complement(2669..3172)
                     /locus_tag="VM910_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVI62137.1"
                     /translation="MIEAGFAQMLALLEEHLASRPYLFGARPAYGDFGLWGQFYQMWR
                     DPTAGALIEGDAPHVLDWIHRMLFPRADGPFEGWATLQSTLAPILKAQVGGKFMPWTL
                     ANEKCLVEGKDEFTVQLGGETWTQKPQKYHARALAMLRAKYAELPDKAVLEPVLREAG
                     CLDGLRT"
     gene            complement(3191..3667)
                     /locus_tag="VM910_06035"
     CDS             complement(3191..3667)
                     /locus_tag="VM910_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI62138.1"
                     /translation="MADLYRIFGAEMSPYSVKVRSYFRYKAIPHQWILRNAASRADYE
                     KHAKVPIIPLVVTPQGIGIQDSTPIIDQMEKLYPEPSIHPEDNVARFVSVLIEEFGDE
                     WGNKWMFHYRWNRDVDQISSAGRIARMQAPHAGEQDHAGLTDKIRTRMTGRVWFVG"
     gene            3783..5564
                     /locus_tag="VM910_06040"
     CDS             3783..5564
                     /locus_tag="VM910_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI62139.1"
                     /translation="MHQPLKHDVAGKSHESGLLAPDTSGMNFYRADPALTDLLHIHLN
                     DALFRHIEPHLDRLGALAGGHLDECARLADRHTPILHQRDRFGRDVQWIEYHPAYREL
                     ERAAFGEFGIHAMSIRKGILGWPDKYPVVAKHAFTFLFNQAEFGLGCPINVTDGCAKL
                     LANFGSEALKEKYLDALTQTDMEKLTQGGQFMTEKEGGSDVGTLTTTAVHEGDHRRLH
                     GEKWFCSNADAKVVMLLARPEGAPPGTRGVGLFLMPRFLDDGSQNHYRIVRLKDKLGT
                     RSMASGEIKLEGAIAYAVGRLDRGFVQMAEMVNSSRLSNGVKSTALMRRAHHDAMTVA
                     KNRVVFGSRIVDLPLGRRQLLKIGLATEQALSMSFLTADALDRAETGSQDAAALLRIL
                     TPTLKFRATRDARKVCGDAMEMRGGIGYIEEFATARLLRDAHLGSIWEGTGNIVALDA
                     LKRAIGRHGAESALAADLHARLDDSGEVPAGWRDRLHGLTDRAVSFAREVAARADNEA
                     EARRATSLLYHVASAVTLAWEAGRIHARRGDARRLLLSRLVVDHRLASGDPFRLAENK
                     VQDAIAGLLLGERDAGMAQVGELVLVA"
     gene            5614..6702
                     /locus_tag="VM910_06045"
     CDS             5614..6702
                     /locus_tag="VM910_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HVI62140.1"
                     /translation="MKAAVLMEVNKPLVIEDVSLSTPGPREVLIRTSVAGLCHSDLHF
                     MEGLYPHPLPAVLGHESAGIVEKVGSDVNYVKPGDHVVTCLSVFCGTCENCTTGRTVL
                     CTDTTVKMLPGASNRLQWSRPEKLNQFLNLSSFAEQMLVHENAIVKIRNDMPLDLAAL
                     IGCGVVTGYGAVVNTAKVQPGESVVIIGCGGVGMAAINGAEIAGAGRIIAVDTNPAKL
                     QLATKLGATDIVDPRNGDVVQQVRDLTKGGVHHAFEVLGRKETAEQAFAMLAPGGTAT
                     IVGMIPFGQKIELHGFDFLRERKIQGSSMGSNHFRVDMPRLVEFYMRGRLHLEDWISA
                     KLKLSEINEGFAAMKAGKTVRSVIMFDA"
     gene            complement(6754..7107)
                     /locus_tag="VM910_06050"
     CDS             complement(6754..7107)
                     /locus_tag="VM910_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI62141.1"
                     /translation="MSKDLDELTALNRDYVASVQNSDVKRFDEILAQDFYCTNPDKSF
                     VDRAAFLKQTAIPVKIKNLKEQDVKIRIFGDFAIIHGQTSYINPDGQQAYGRYTDCWA
                     KQNGKWLAVSAHVAR"
     gene            complement(7122..8594)
                     /locus_tag="VM910_06055"
     CDS             complement(7122..8594)
                     /locus_tag="VM910_06055"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI62142.1"
                     /translation="MAKSQWSFKSAIEISAALKARKVSSVELTEDAIGRIERHDGKIN
                     AICVRDFSRALDAARAADAALGRGEHRPLLGIPLTVKESFNIAGLPTTWGFPEQKNFV
                     PQQDALSVTRMKEAGGVILGKTNVPIGLGDWQSYNDIYGTTNNPYDLARTPGGSSGGS
                     SAALAAGYGPLSIGTDIGGSLRVPAFHCGVFAHKPTFGLVASRGHTPPPLPPLPFDRD
                     LSVLGPMARSAADLSLLLDVIAGPDPIEAGKAYRLELPPPRHTALKDFRVLLIETDPL
                     LPTDKDVRAGIERLADHLGKAGVPVTRKSPLWPDFAASSRLYMRMLLSFLGATFQPDI
                     YAGAQAAAAQLASDDISLRAERLRGIALSYRERVMAEGGRTRLRAQWRELFNSFDAVI
                     CPIMPTPAYVQDHAPEQETRRINIDGKEYSYPDQLAWPGVATLPGLPATAIPTGFSPA
                     GLPIGVQIVGPFLEDRTPLKLAELIEREFCGFVPPPMFDD"
     gene            complement(8774..9307)
                     /locus_tag="VM910_06060"
     CDS             complement(8774..9307)
                     /locus_tag="VM910_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62143.1"
                     /translation="MRTLFVSPHADQRDFFYGINFEFANETPPFAQTRFAMEIRPIIG
                     GRNSEYEFIVNPIVDIGFGRFGEAHFAPAARVARKFGDDLYAGFEYYSDWGSIGDFKA
                     FAQQEHALFAVTDFKLGVVDVNFGVGYGLTPASDRWIVKTILGYAFPVPGSNTAGSDR
                     ATTGPVNPMSRATQRTW"
BASE COUNT         1759 a         2926 c         3062 g         1744 t
ORIGIN      
        1 gagcagccat tcccggaatg ccttgagctt gggcgggtcg gccgtttccg gcgaaaccag
       61 atagaacccg gcatccgccg gcagcgtgat cttgaaggga accacaaggc ggcccttggc
      121 gacgtcgtcc tggacatagg aggtgcggcc gactgcgacc ccgatgccgt cgatggcggc
      181 ctgcacggtc atcaggctca ggtcgaaggt gatgcccggc tgcttcgaaa tgtcggtggg
      241 cagcccggcc gccgtcaacc acagccgcca atcgtcgttg ttgccgccgg tggtgtgcag
      301 cagcacatga tctttcaggt cttccggcgt ccgcagcggc cgcttgccat tcaagagctg
      361 ggggctgcag acgggaaaca gctcgtctgc catcagccag tcggcgcgca ggcccggcca
      421 atggccgcgg ccgtagcgga tcgcggcatc gacatcgccg cttttgaaat ccacgagatt
      481 ggcggaggtc gtgatgcgga cgtcgatctt ggggtgcgcc tcctgaaagg tcgacagccg
      541 cggcagcagc catttgaccg cgagcgaggc gagcgtcgag acggtcagga catggtcgtc
      601 gtccttgcgc aacaaccggt cggtcgcaag cctcagatcg ttgaaggcgg cacgcacgcc
      661 cgggaggtag tcgcgcgcct tcggcgtcag agccagcgcc cggttcttcc ggatgaacag
      721 tcggacgcca agctcctcct ccagccgccg gatctgatgg ctgatcgccg tctgggtcac
      781 gttcaactcg gaggcggcct gggtgaagct caaatgccgc gcggcggctt cgaacgcgcg
      841 cagaccgttg agggagggca gcctggcggt cacgatgcgg tgatccaatt catgagctta
      901 tttcatgcga agcggtacaa agtgtcgttt gcggaacggt ccaatagctc agatattggc
      961 tgcgaaagat tagctttagg agccgaaaat gtctacctac acgcaccaaa cgatgacaaa
     1021 tcatcatggt ctgggcattt tggcccattt gagcgagacc attcatacgt ggcggcagcg
     1081 ttacgagacc cgccgcgagc tcacccaatg gaccgaccgg gacctgcacg atgtcgggct
     1141 gtcgtggagc gacgtcgctc gcgaagccga gaagcccttc tggcgggctt gatgagccgc
     1201 caatgccggc gccgcctcga cggggggcgc cggccatttc tttcctgaag agcgggagtt
     1261 tgccatgacc aggcttcggt tcgacgacct caaacagtat tccgacgtcc tgcgctcgcg
     1321 ccgcggcgac ccgatcacgg tacgttttgt cgagccgcgc gatgccgaag agctgcagaa
     1381 ctattttcgc tcgctgtcgt cccgctcccg ctacaaccgc tttctgggcg cgatgagcga
     1441 gctgccgaag ccagtactcg acaatttcac ccatgtcagc gagcgcgacg gctttaccgt
     1501 gatcgcgacg atccaggtcg acgggttcga gaccatcgtc ggcgaggccc gctacatctt
     1561 taatcccgcg gacggcagtt tcgaatttgg cctgtcgatc gaggatcgtt ggcaagggtc
     1621 tggcatcggc tcggcgcttc tgaagaatct ggaatgtcgc gccgctgcat tcggcgcgga
     1681 gcggttgttc ggcgacacgc tgcgctccaa tcagagcatg attgcgttgg cgcgcaagtc
     1741 gggctttgcc ttcaccaaga atccggacga ttggaagctg gtgcgcttca ccaaggagat
     1801 cgccgttgcg cctgaagaga ttccatgcgc gagctggcgg atcgcggcgc agaacgcggc
     1861 gttgaccccg gtggtcgcag gctagcgaaa cagcggttgc ccgcttcgat gagcgcggca
     1921 tcgttccctc aacgtcagaa cttctccacc cacggtcgca gctcgatttc ccaggtccag
     1981 gcgctgcgcg gctgttgcaa tacgttccag taactcaggg cgattgcgtc cggatcgagc
     2041 catgaatccg gccgatcggg cgggtcctgg cgtgcggcgc tgcggatacc gccgtcgatg
     2101 acgaaatgtc cgatgtggat gccctgcggc gacaattcgc gcgccaaaga ctgcgcaagt
     2161 ccccgcaatg cgaacttgcc catcgcaaag ggcgaggatt gcgcatagcc cttgacgctt
     2221 gctgaggcgc cggtgaagag gatcgcgccg tgcttgttcg gcagcatgcg ccgcgccgcc
     2281 tgctgggcca caagaaagcc gccgaacgcg ctgatcagca gggcctgctc aacctccgcc
     2341 ggaactagat cgacaaacgc gccgcgcgcg cgggcgccgg cattgtagac cacaaggtca
     2401 ggcgcgccga tctcccgctc cacaaggcca aacaggcgtt cgacctggtc ggcatcagcc
     2461 gcatcgcagg cataggcacg tgcgccggtc tcgcggcaaa gcgcgccgag cttttcgatc
     2521 ttgcgcgcag cgagcgcgac attgatcttc tcgcgggcga acagccgcgc cagcgacgcg
     2581 ctcaggcctt cgcccacgcc gacgatgaga gcggtcttgt aggttggcgt ttccatgtgg
     2641 gacttccttt cctctccgtc gagagtatct aggtccgcaa tccgtccaga caacccgcct
     2701 ctcgcagcac cggttcgagc acggccttgt ccggcagctc tgcatatttt gcgcgcagca
     2761 tcgcaagcgc gcgcgcatga tatttctgcg gcttctgcgt ccaggtctcg ccaccaagct
     2821 gcacggtgaa ttcatccttg ccctcgacaa gacatttctc gtttgccagc gtccagggca
     2881 tgaattttcc gccgacctga gctttgagga tcggcgcgag cgtcgactgc aacgtggccc
     2941 agccttcgaa ggggccgtcg gcgcggggga aaagcatgcg atggatccag tcgagcacat
     3001 gcggcgcgtc gccttcgatc agcgcgccgg ccgtcggatc tctccacatc tgataaaact
     3061 gtccccacag cccgaagtcg ccgtaagcgg ggcgcgcgcc gaacagatag ggtcggctgg
     3121 cgaggtgctc ttcgagcaaa gcgagcatct gggcaaagcc ggcttcgatc agcggcgcgg
     3181 tttcggcgtt ctagccgacg aaccagaccc ggcccgtcat gcgcgtgcgg attttgtcgg
     3241 taaggccggc atggtcctgc tcgcccgcgt gaggggcttg catacgcgcg atccgcccgg
     3301 cggacgagat ctggtcgacg tcgcggttcc agcggtagtg aaacatccat ttgttgcccc
     3361 actcgtcgcc gaactcctcg atcagcacgg agacgaagcg cgcaacgtta tcctcgggat
     3421 ggatcgaagg ctccggatag agcttctcca tctggtcgat gatcggggtg gaatcctgga
     3481 tgccaatgcc ttgcggcgtc accaccagcg ggatgatggg caccttggcg tgcttttcgt
     3541 agtcggcccg gcttgccgcg ttacgcaaaa tccattgatg cggaatggct ttgtagcgaa
     3601 aatacgagcg caccttgacc gaatagggcg acatctcggc accgaagatg cgatagaggt
     3661 cagccatagg atcatgcctc cctgagcttg tgacggcttc acgcgccgcg cgtctcgtgg
     3721 aacacgatct acatcatcca aagccggcaa gacagggcgg aaccgaccag ggagattcga
     3781 aaatgcacca gccgctcaag cacgacgtgg cgggcaagtc gcatgaatcc gggttgctcg
     3841 cgcccgacac atcgggcatg aatttttacc gcgccgatcc cgcgctgacc gacctgcttc
     3901 acatccatct gaacgatgcg ctgtttcgcc atatcgagcc gcatctcgac aggctcggcg
     3961 cgctggccgg cggtcacctc gacgaatgcg cgcggctcgc cgaccgtcac acgccgatcc
     4021 tgcatcagcg cgaccggttc ggccgcgacg tgcagtggat cgaatatcat ccggcctatc
     4081 gcgagctcga gcgcgccgcc ttcggcgagt tcggtattca cgcgatgtcg atccgcaagg
     4141 gcattctggg ctggccggac aagtatccgg tcgtcgccaa gcacgccttc actttcctgt
     4201 tcaaccaggc cgagttcggc ctcggctgtc ccatcaacgt caccgatggc tgcgcaaagc
     4261 tgctcgccaa tttcggcagc gaagcgctga aagaaaaata tctggacgct ctcacccaga
     4321 cggacatgga gaagctgacc cagggcggcc agttcatgac ggagaaggag ggtggctccg
     4381 acgtcggcac gctcaccacg acggcggtgc acgaaggcga tcaccggcgc cttcatggcg
     4441 agaagtggtt ctgctccaac gcagatgcaa aagtggtgat gctgttggcg cgcccggaag
     4501 gagcgcctcc gggtacgcgt ggcgtcggcc tgttcctgat gccgcgcttt ctcgacgacg
     4561 gctcgcaaaa tcattaccgg atcgtgcggc tgaaggacaa gcttggcacc cgttcgatgg
     4621 cctccggcga gatcaagctc gaaggcgcga tcgcctatgc ggtcggccgg ctcgaccgcg
     4681 gtttcgtgca gatggccgaa atggtcaact cgtctcggct ctccaacggc gtcaaatcga
     4741 cggcgctgat gcgccgcgcg catcacgacg ccatgacggt tgcgaaaaac cgcgtggtgt
     4801 tcggcagccg tatcgttgac ctgccgctcg gacggcgcca attgctgaag atcgggcttg
     4861 ccaccgagca ggcgctgtcg atgagctttc tgaccgccga cgcgctggac cgagccgaga
     4921 cgggcagcca ggacgccgcg gcgctgctgc ggattctcac gccgaccctg aaattccgtg
     4981 ccacgcgcga tgcgcgcaag gtctgcggcg atgccatgga gatgcgcggc ggcatcggct
     5041 acatcgagga gttcgcgact gcgcgcttgc tgcgcgacgc gcatctcggc tcgatctggg
     5101 agggcaccgg caacatcgtg gcgctggatg cgctgaagcg ggcgatcggc cgtcatggcg
     5161 ccgagtcggc gctggcggcg gatttgcacg cccgtctcga cgacagcggc gaagtgccgg
     5221 caggttggcg cgatcgcctg cacgggctga ccgatcgtgc ggtctccttt gcgcgcgagg
     5281 tggccgcgcg cgccgacaac gaggcggaag cccggcgcgc caccagcctg ctttatcacg
     5341 tcgcaagcgc ggtaacgctc gcctgggaag ctggccgaat ccatgcgcgg cgcggtgacg
     5401 cgcgccggct cttgctgtcg cggcttgtcg tcgatcatcg gctcgcctca ggcgatccgt
     5461 tccggctcgc ggaaaataag gtgcaagacg cgatcgcggg tctcctcctt ggcgagcgcg
     5521 atgccggcat ggcccaggtt ggcgaattgg ttctggtggc gtaggttctt cgccacgcgc
     5581 gttccgacca aacaagaatc gagggagaaa ccgatgaagg ctgccgtcct gatggaagtc
     5641 aacaagccgc tcgttatcga ggacgtcagc ctgtcgactc ccggcccgcg cgaggtcctg
     5701 atccgcacct cagttgccgg cctctgccat tccgacctgc atttcatgga agggctttat
     5761 ccgcatccgc tgccggcagt gcttggccac gagtccgcag gtatcgtcga gaaggtcggc
     5821 tccgacgtca attatgtgaa gccgggcgat cacgtcgtca cctgcctctc ggtcttctgc
     5881 ggcacctgcg agaattgcac cacgggacgg acggtgctgt gcaccgacac cacggtgaag
     5941 atgctgccgg gcgcctccaa ccgcctgcaa tggtcgcggc cggagaaact caatcagttc
     6001 ctcaatctct cgtcattcgc cgagcagatg ctcgtgcacg aaaacgccat cgtgaagatc
     6061 cgcaacgaca tgccgctcga cctggcggcc ctgatcggct gcggcgtcgt caccggctat
     6121 ggagccgtgg tgaacacggc gaaggttcag cccggcgaga gcgtggttat catcggttgc
     6181 ggcggcgtcg gcatggccgc gatcaatggc gcggagatcg cgggtgccgg gcgcatcatc
     6241 gcggtcgaca ccaatccggc aaaactgcag ctcgcgacga aactcggtgc aaccgacatc
     6301 gtcgatcccc ggaacggcga cgtcgtgcag caggtgcgcg acctgactaa aggcggcgtg
     6361 catcatgctt tcgaggtgct cggacgcaag gagaccgccg agcaggcctt tgcgatgctc
     6421 gcgccagggg ggactgcgac catcgtcggc atgatcccgt tcggacagaa gattgagctt
     6481 cacggtttcg atttcctgcg cgagcgcaag atccagggtt cgtcgatggg ttcgaaccat
     6541 ttccgcgtcg acatgccgcg gctcgtcgaa ttctacatgc gtggccggct gcatctggaa
     6601 gactggatct cggccaagct gaaattgtcc gagatcaacg agggttttgc cgccatgaag
     6661 gccggcaaga ccgtgcgtag cgtcatcatg ttcgatgcgt gagacgtttt gctcgaagat
     6721 gctctcccgc gcctttgccg gcagtgaatt gggtcaccgc gcgacatggg ctgaaacggc
     6781 cagccacttc ccgttctgct tggcccagca gtcggtatag cgcccgtagg cctgctggcc
     6841 atcagggttg atgtagctgg tctggccgtg gatgatcgcg aagtcgccga agatgcgaat
     6901 cttgacgtcc tgctccttca gattcttgat cttcacgggg atcgcggtct gtttcaggaa
     6961 cgcggcacga tcgacaaagc tcttgtcggg attggtgcaa tagaaatcct gcgccaggat
     7021 ttcgtcgaag cgcttaacgt cagagttttg caccgacgcg acatagtcgc ggttgagcgc
     7081 cgtcagctcg tcgaggtcct tgctcattgc gtttctccct ttcaatcatc gaacatcggc
     7141 ggcggcacga agccgcagaa ctcgcgctcg atcagttctg ccagtttaag cggcgtgcgg
     7201 tcctcgagga aggggccgac gatctgcacg ccgatcggca acccggcggg cgaaaacccg
     7261 gtcgggatcg cggtcgcggg gaggccgggc aatgtcgcga cgccgggcca ggcgagctga
     7321 tcgggatagg aatattcctt gccgtcgatg ttgatgcggc gtgtctcctg ctccggcgcg
     7381 tgatcctgca cataggccgg cgtcggcatg atcgggcaga tgacagcgtc aaagctgttg
     7441 aacaattcgc gccattgcgc gcggagccgc gtgcggccgc cttcggccat cacccgctcg
     7501 cggtagctga gcgcaatccc tcgcagccgc tcggcgcgaa ggcttatgtc gtcggacgcg
     7561 agctgggccg cggcggcctg cgcgccggca tagatatcgg gctggaaggt cgccccgagg
     7621 aacgacagga gcatccgcat ataaagacgc gacgaggcgg cgaagtccgg ccagagtggg
     7681 cttttgcgcg taactggcac gccggctttg cccaaatggt cggccagtcg ctcgatcccg
     7741 gcgcgcacgt ccttgtcggt cggcaacagc ggatcggtct cgatcaaaag cacgcgaaaa
     7801 tccttcagcg cggtatggcg cggcggcggc agctccagtc gataggcctt gccggcttcg
     7861 atcggatcgg ggcccgcgat cacgtcaagc aacagtgaga gatcggccgc cgagcgcgcc
     7921 atcggaccga gcacagagag gtcgcggtcg aagggcagcg gcggcagcgg cggcggcgtg
     7981 tgaccgcgcg aggccacgag cccgaaggtt ggcttgtggg caaagacgcc gcagtgaaag
     8041 gccggcacgc gcaaggagcc gccaatgtcg gtaccgatcg acaacgggcc atagcccgcg
     8101 gcgagcgcgg ccgacgatcc gccggaggag ccgccgggcg tccgggcgag atcataggga
     8161 ttattggtgg tgccgtagat gtcgttatag ctttgccagt cgcccaatcc gatcggcacg
     8221 ttggtcttgc cgaggatcac gccgccggcc tccttcatgc gcgtgactga cagcgcgtct
     8281 tgctgcggta cgaaattctt ttgctcggga aatccccagg tcgtgggcag gcccgcgata
     8341 ttgaaggact ccttcaccgt caggggaata ccgagcagcg gcctgtgctc gccgcggccc
     8401 agcgccgcat cggcggcccg cgctgcatcg agcgcgcggg aaaagtcgcg cacgcagatc
     8461 gcgttgatct tgccgtcgtg ccgctcgatg cgaccgatgg cgtcctcggt cagttcgacc
     8521 gaggaaacct ttcgtgcctt gagcgccgcc gaaatctcga tcgcgctctt gaagctccat
     8581 tgcgatttgg ccacgccgca ctccctgttg tccgacttca gtgccgatga atgatgatca
     8641 gtatggcgga ggacggcaag atgcagcttg atcgcaattg ccatgcagaa aaaattcccc
     8701 cgcatcatcg gctgcaagac ttgctcgcga tagacgagcg gtcatcggac aatccagcat
     8761 tcgcgaagga gggtcaccat gtgcgctgcg tcgcgcgcga catcgggttg accgggccgg
     8821 tcgtggcgcg gtcgctgccg gccgtattgc ttccggggac cggaaacgcg tagccgagga
     8881 tggtcttgac gatccagcgg tcggaggcag gggtcaggcc gtagcctacg ccgaaattca
     8941 cgtccaccac gccgagcttg aaatcggtca ccgcaaagag cgcatgctcc tgttgggcaa
     9001 acgccttgaa gtctccgatg ctgccccaat ccgaataata ttcgaagccc gcgtagagat
     9061 cgtcgccaaa cttgcgcgcg acccgcgcgg caggtgcgaa atgcgcctcg ccgaatcggc
     9121 cgaagccgat atccacgatc gggttgacga taaattcata ttcggaattg cgcccgccga
     9181 tgattgggcg gatttccatg gcaaagcggg tctgcgcgaa gggcggcgtc tcgttcgcga
     9241 attcgaaatt gataccgtag aagaagtcgc gttggtcggc atgcggcgac acgaacagcg
     9301 tccgcacctt gaaagcgttg gaatagaagg tctggttcta tgtcatcaag gccttgggcg
     9361 ctgctctttg tgttgaattt caatcgcttg tcgaaaggcc catcgcgaac gcaaagggaa
     9421 catggaccca gcttggaccc aaacgggtta cggcttgaga aagccgccgg aaaaagatgc
     9481 aagcgcggag g
//
LOCUS       DATLJT010000267         1760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4379274,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000267 DATLJT010000000
VERSION     DATLJT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4379274"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..614
                     /locus_tag="VM910_06065"
     CDS             150..614
                     /locus_tag="VM910_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62144.1"
                     /translation="MNEARLAADLGELAVDYSDAQLKDLRIGTLLHRVSAILREHSIV
                     LPVDLALLFKALITLEGFGRQYDPEFRLVARAEPFLNRACPSAISRWKRGDRAKRRSA
                     TSWGWSPRSRVTLPGWSRTRAMGACGSTSILNASTPSVIAYTMRSIARRSAS"
     gene            611..778
                     /locus_tag="VM910_06070"
     CDS             611..778
                     /locus_tag="VM910_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62145.1"
                     /translation="MTASLVVGSSIVMTVSGGPTVFGVSLLTYCGLVGYLIAFVNSLW
                     VILSIWRSGRR"
     gene            complement(873..1484)
                     /locus_tag="VM910_06075"
     CDS             complement(873..1484)
                     /locus_tag="VM910_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HVI62146.1"
                     /translation="MTSRDTILAAVRANQPAPACPLPEIPLFEGITGSLLGDFQEALL
                     RMGGKFVDQRSGKNLEAFVRSLFPQATVICSATAEVKGARALTSNASPTDLADVDVGI
                     VRAAFGVAETGSIWLSETELQVNALAYLAQHLVALLDPSDIVGNLHHAYRDPRFKTAR
                     YAVLVTGPSATADIEGVLIHGAQGVRSLTVLLVPRPAKPRATV"
     gene            complement(1481..>1760)
                     /locus_tag="VM910_06080"
     CDS             complement(1481..>1760)
                     /locus_tag="VM910_06080"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HVI62147.1"
                     /translation="INIHEQIYKWRQIIAERNQLPFVKKEMMRVAGKVLADPRLYRAA
                     ISAAGLAIEHLPRFAIYNWLNPWGRQREIPEAPQQTFRSWYIEHRRRA"
BASE COUNT          302 a          544 c          562 g          352 t
ORIGIN      
        1 cgatccgcat cccggcaatg tgatgtgcct gcccggcaac cgcatcgcgc tgatcgattt
       61 tgggatggtg ggtcggctgt cgccggcgcg tcgcagccag ctcgtcaatc tgctcgctgg
      121 gttcgtgcac caggacgaag aatgaagcgg tgaacgaagc caggcttgcc gccgatctcg
      181 gcgaactggc tgtcgactat tccgacgcgc agttaaagga tctgaggatc gggacgctgc
      241 tgcaccgagt atcggcgatc ctgcgtgagc actcgatcgt gctgccggtg gatctcgctc
      301 tcttgttcaa ggcgctcatc actctcgaag gattcggccg ccagtacgat ccggagttcc
      361 gtctggtcgc gcgcgctgag ccgtttctca accgcgcatg tccgagcgct atcagccggt
      421 ggaagcgtgg cgacagggcc aagcgacgtt cagcgacttc ttggggctgg tcgcctcgat
      481 cccgcgtgac cttgcccggt tggtcaagga cgcgcgccat gggcgcatgc ggatcgacct
      541 cgatcttaaa cgcctcgacg ccttcggtga tcgcgtacac gatgcgctcg atcgcgcgac
      601 gatcggcatc atgacggcat cgcttgtggt cggctcatcc atcgtaatga cggtgtcggg
      661 agggccgacc gtgttcgggg tgtccctgct tacctactgc gggctggtcg gctacctgat
      721 cgcgttcgtc aacagcctct gggttatcct ttccatttgg cgctccggac ggcgatgact
      781 gaacgccggg caatgcctac tttggctgtg cctccatccg accggccgcg tggcggttgg
      841 tgatctctga ggcgatcggc tggttcgccg ggctagacag tggcgcgagg cttcgcaggg
      901 cgcggaacca ggagtaccgt cagactccgc acgccctggg ccccgtgaat caagacgccc
      961 tcgatatcgg cagttgcaga gggacccgtt accagcaccg cgtagcgggc cgtcttgaac
     1021 cgcggatcgc gataggcgtg atgtagattt ccgacgatgt cagaagggtc gagcaaagca
     1081 acgaggtgct gcgcaagata ggcgagcgca ttcacctgca gctcggtctc gctgagccag
     1141 atcgaccccg tttcggcgac cccgaaggcg gcgcggacga tcccgacatc gacatcggcc
     1201 aaatcggttg gactggcgtt gctggtcaag gctcgagctc ccttcacctc cgccgtggcc
     1261 gagcagatca ccgtcgcctg cgggaaaagc gagcggacga aggcttcaag gttcttgccg
     1321 cttcgctgat ccacgaactt tcctcccatc cgcagcagcg cttcttggaa atcgcccaac
     1381 agcgatccag tgatcccttc gaacagcggg atctccggaa gcgggcaggc aggcgcaggc
     1441 tggttcgcgc gtaccgctgc aaggatcgtg tcgcggctcg tcacgcgcgc ctcctgtgct
     1501 cgatatacca gctgcgaaag gtttgctgcg gcgcctctgg aatctcgcgc tggcggcccc
     1561 acggattgag ccaattgtag atggcgaagc gcgggagatg ctcgatcgca aggccagccg
     1621 ccgagatggc cgcgcgatac agcctcgggt cggccagcac cttgccggcg acgcgcatca
     1681 tttctttttt cacgaacgga agctggttgc gctcggcaat gatctgccgc catttataga
     1741 tctgctcgtg aatgttgatc
//
LOCUS       DATLJT010000268         1784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4150147,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000268 DATLJT010000000
VERSION     DATLJT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4150147"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VM910_06085"
     CDS             <1..336
                     /locus_tag="VM910_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62148.1"
                     /translation="LALQDVAAGPGGEPYFPTPWASNKSISFLFTPQSTRAALERIGF
                     RVLAWHDTTQEALEQQLARAKALESGSLPPLGLHILIGEAYPAVTKNMLRNLQEHRIE
                     LLNAVLQRV"
     gene            complement(410..805)
                     /locus_tag="VM910_06090"
     CDS             complement(410..805)
                     /locus_tag="VM910_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI62149.1"
                     /translation="MATSVKQMMEAANAAVPRITLAQAREMIAKGNTLVVDVRDAPEV
                     EKSGKVAGAVNVSRGMLEFRADPESPYHDKNFAKDKTVILYCASGGRSALGGKVLKDM
                     GYQQVYNLGAFKDWAEGGGAVDKPTEPGT"
     gene            1125..1532
                     /locus_tag="VM910_06095"
     CDS             1125..1532
                     /locus_tag="VM910_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62150.1"
                     /translation="MSRTGNRFGPQLLLLAFALTLSACAGASRSRDVRDPRPENYKSD
                     ILAMLPVYLRDPTQIRDASASEPTPQLVGTTNRYVVCLRFNAKKSNGEYAGLKQYAAI
                     FVAGRLDQMVEVKPEQCATADYQPFPEAERLTR"
BASE COUNT          319 a          576 c          541 g          348 t
ORIGIN      
        1 ctcgcgctcc aggacgtcgc ggcggggccg ggcggcgagc cgtatttccc cacgccgtgg
       61 gcaagcaaca aaagcatcag tttcctgttt accccgcagt cgacccgggc cgcgctcgag
      121 cgcatcggct ttcgcgttct cgcctggcac gacacaacgc aggaggcgtt ggagcagcag
      181 cttgcgcgcg ccaaggcgct ggagagcgga tcactgcccc cgcttggcct tcacatcctg
      241 atcggggagg cttatccggc ggtgaccaaa aacatgctgc gcaatctgca ggagcacagg
      301 atcgaacttc tcaacgcggt gctgcagcgg gtctagcgcc agatatggcg atggcgctgg
      361 cgcgatgggc gacgagcgac ttgcggtcca tccatcgacc gcgcgacgct cacgtgccgg
      421 gctcggtcgg cttgtcgacc gcgcccccgc cttcggccca atccttgaac gcaccgaggt
      481 tgtagacctg ctggtagccc atgtctttga gcaccttgcc gcccaaggcc gagcgaccgc
      541 cggaggcgca gtaaagaatg accgttttgt ccttggcgaa attcttgtca tgatacggcg
      601 actcgggatc ggcgcgaaat tccagcatgc cgcgcgagac gttcacggcg ccggcaacct
      661 tgccgctttt ttccacttcc ggagcgtcgc gcacgtcgac cacgagggtg ttgcccttgg
      721 cgatcatctc gcgcgcctgc gccagcgtga tccgcggcac cgcggcgttg gcagcttcca
      781 tcatctgctt gacgcttgtg gccatggcgt cctcctctct cccggcattc gggcccgtca
      841 gccgcggatg tgctccgcag cctcgggacg acggcacgat atcacacctc gcatggcgcc
      901 gtaggggcgt cgaaaagccg cgcggctgaa cgatgaccgg tccgagtcag tcacgcctgg
      961 ggttgaccga ggtcgcacgt ccatcccctt ccgcagcgcc tcaagaccgg acataaatca
     1021 atgttttggc gccgacgcga tgctcactcg ggcatcgtca tgctcatcgg ggcgacgaca
     1081 ggtcgtgatc ggcggccacc tacctctgcg atgggaaggc agccgtgagc agaaccggca
     1141 accgttttgg gcctcaattg ttgctcttag cttttgcgct cacgttgagc gcgtgcgccg
     1201 gcgcaagtcg cagtcgcgac gtccgagatc ctcgtccgga aaactacaag tcggacattc
     1261 tcgccatgtt gcccgtctat cttagggatc cgacccaaat ccgcgacgcc tccgcgtccg
     1321 aaccgacgcc tcaactggtc ggcacaacaa atcggtacgt cgtctgtctt cgattcaatg
     1381 ccaagaaaag caatggcgaa tatgccggcc tgaagcaata cgcggctatt tttgttgccg
     1441 gcaggctgga ccagatggtg gaggtcaagc ctgagcaatg cgccacggct gactatcagc
     1501 catttcctga agcggaaagg ttgactcgat aaccgcggtt ctgggattgc gcggctcctc
     1561 ttgtgtccat cacgattcat ggatgtaatt tgcatcgaca ggaacggcat atgatgaatc
     1621 gtctgcgatt gggcctgctc ggcacccttc tcgtcagcgc aatttggccc gcggctgcgc
     1681 agccgcagaa ctacccgacc cggacgatca cgttcgtcgt gccattcgcc gcgggtggct
     1741 tgaccgacgt tccggcccgc atcctggcgg cgatgatgca ggag
//
LOCUS       DATLJT010000269         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4150346,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000269 DATLJT010000000
VERSION     DATLJT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4150346"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VM910_06100"
     CDS             complement(<1..985)
                     /locus_tag="VM910_06100"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62151.1"
                     /translation="MASYVARRKFLATLGGAAVGWPLAARAQHRAIPVVGFLGSPSAA
                     EWAPFVTAFQRGLKETGFVEGENVAIEYRWANGHYDRLPAMVADLVHLQAAVIFAAGS
                     PAPALAAKAATATIPIVFANGADPVQFGLVASLNRPGGNITGVNFLAADLGQKGLGLI
                     HELLPNVTVAAMLVNPNNPNADSVARNARETAASLGLQLHILNAGTIGEIDTAFAKLV
                     DEHIGMLVLGADALFLGRREQFVTLAAHNAIPAIYFARELVTAGGLMSYGTSVSDAYH
                     RAGVYTAKILKGAKPADLPVEQSTRFEFVINLKTARTLGLTIPDRLLALADE"
     gene            complement(1155..1421)
                     /locus_tag="VM910_06105"
     CDS             complement(1155..1421)
                     /locus_tag="VM910_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62152.1"
                     /translation="MAGDVLALRGVWELAKRAYASVVEERDELLKQLYWHQDQLRELT
                     ETLRELKAVVLARQHAETELAALYRERAIQRARSAERDPAAPLQ"
     gene            complement(1481..1772)
                     /locus_tag="VM910_06110"
                     /pseudo
     CDS             complement(1481..1772)
                     /locus_tag="VM910_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335275.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1907..2833)
                     /locus_tag="VM910_06115"
                     /pseudo
     CDS             complement(1907..2833)
                     /locus_tag="VM910_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2830..>3438)
                     /locus_tag="VM910_06120"
     CDS             complement(2830..>3438)
                     /locus_tag="VM910_06120"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62153.1"
                     /translation="TGLVASLARPGGNVTGLANQISDTSGKKLEFLREVVPGLRRLAI
                     MANAGNPASVLEMREVQATARALGLEITTSEIRRAEDIVPAFETLKGRADALYLCPDP
                     LMNTNRTRINILAVGARLPTMHGAREYVEAGGLISYGANLPDHFRRAAELVDKILRGA
                     KPADLPVEQPTKFNLIINLTTAKALGLEIPPTLLARADEVIE"
BASE COUNT          662 a         1066 c         1052 g          658 t
ORIGIN      
        1 cctcgtcggc cagcgcgagc aaacggtctg ggatggtgag gcccagcgtc cttgcggttt
       61 ttagattgat cacgaactcg aacctcgtcg actgttcgac tggcaggtcg gccggctttg
      121 cgcctttgag gatctttgcg gtgtagacac cggcccggtg atacgcgtcg cttacgctag
      181 tcccatagct catcaagccg ccagcggtga ccagttcgcg cgcgaagtaa atcgcaggaa
      241 tggcgttatg cgccgcgagt gtcacgaact gctcgcgccg gccgagaaac agggcatcgg
      301 cgccgagcac gagcatgcca atatgctcat cgacaagctt tgcgaaggcg gtgtcgatct
      361 cgcctatcgt gccggcattc aagatatgga gttgcagccc cagcgaggct gccgtctcgc
      421 gcgcgtttct cgcaaccgaa tcggcatttg ggttgttagg atttaccagc atggcggcga
      481 cggtcacgtt gggaaggagc tcatggatca agccgagccc tttttgtccc aagtcggcgg
      541 caagaaagtt cacgccggta atattgccgc caggccgatt gagactggcg accaagccga
      601 attgaaccgg atcggcacca ttggcaaaga cgatcggaat agtcgccgtc gctgccttcg
      661 cggcaagcgc tggagcggga gaacctgcgg caaagatcac ggccgcttga aggtgaacca
      721 aatcggccac catcgcgggc aggcgatcat aatgcccgtt cgcccagcgg tattcgatgg
      781 cgacattttc gccttcgaca aaacccgttt ctttcagacc gcgttggaat gcggtcacga
      841 acggagccca ttctgcggcg gatgggctac caagaaagcc gaccactggg atagcccgat
      901 gctgcgcgcg cgccgcgagc ggccagccga cggccgcgcc gccgagcgtg gctaagaatt
      961 ttcgtcgtgc gacgtagctt gccatgtgac ccccccggtt ggggtcattg atgcaatgga
     1021 gggatgatac tacgcttcca tcgcgcggtc ttggactaag gccgataggc aaaatcgcgt
     1081 ctggggcaaa ggcggtaagg ctcagaggga gcaaatgttt agcggcttgc ccccaaagcg
     1141 gccgtatact ggcgtcattg cagcggtgct gccgggtcac gctcggcgct acgcgcacgc
     1201 tggatcgcac gctcgcgata cagcgcggca agctcagtct cagcatgctg gcgggcaagc
     1261 acaacagcct tcaactcgcg tagtgtttcg gtaagctcgc gaagctggtc ctgatgccag
     1321 taaagttgtt taagaagttc gtcgcgctct tccacaacgg aggcataagc acgcttggct
     1381 aattcccaaa ctccgcgcaa tgctaagacg tcgcctgcca tcccgcattc agcgtccctt
     1441 acgcgggttt gcgagaaggg cctgttaggt ccaaatgccc ctcgcagatc gcttccgcat
     1501 cggcggcatc gtgcttgttg cgcttgatgt acgccttcac gtattgcgcc ggcatcagtc
     1561 gaacctcgtg gccaagcgcg cgcaactccc gcgcccagta gtgtgccgtc gcgcacgcct
     1621 ccaggccaac cgggcggcag tcgactgaag aacgccagaa cctgcgcacg ccgaagctgc
     1681 ttgcgcagaa ccgtcgctcc ctctgcatcg accccgtgca cctgaaaaac atgcttcgcc
     1741 aagtcaaatc cgatcgtagt aacctcgccc atggatggct cccgttcctg gtgattcttc
     1801 gaaacaccag tttggcacgt cgatgccgca agggggccat ccaccccatc actcgatcac
     1861 ctcgtcggcg gccacgagca gaccggggcg ccacgtcgag gccgagcgcc ctagccgttt
     1921 gcagattgat aacaaactcg aacttggtgg gctgcgttac cggcaagtca gcgggcgttt
     1981 cccccttgag gatgcggcca acatagaccc cgacctcgcg aaacgaatcg gtcacgtttg
     2041 ttccatagct catcagtccg ccagcttcgg cgtagtcgcg attgggatag gtggcgggaa
     2101 tcccgtttcg tgccgccaaa atggcaagtt gcacgcgtcg gctgtggaag aaaaattcgg
     2161 gggcgacgaa cagcgcttcg gcgcgctcgc gcgacatcgc ggcgaaagcc gcatcgatct
     2221 cgccgctgtt gctcgcgttg aggacctgga ttttcagccc gaaagcgtct gccgctttct
     2281 gcatctctcg cacagtggtc tcggcactca caacgttgcc cggattgacc atcgcggcga
     2341 cgcgaaccgc tccaggcacc agaccgcgca gcaaccccag tcgctttggc accgcttcgc
     2401 tgacgaagaa attgatgccg gtcgcgttgc cacctggccg cgcgaggtta gcgaccaggc
     2461 caagcttgac cgggtcgtcg gtgacgccaa aaacgattgg gatcgtcgcc gttgcagcct
     2521 tgaccgccaa cgccgctggt gtactgcccg gcgcggcgat cacggctacg cgacggcgaa
     2581 ccaattcggc cgccagagcc ggtagttgat cgtattgacc ttccgccgaa cgatactcga
     2641 ccgtcacgtt ctgaccttcg atgtagccag cgtcagtgag gcctttgcgg aaggcggccg
     2701 cttcctgcgc ggagccaacg gctgacgcgc cgttgatgaa cccaaccacc ggcatcgccg
     2761 gttgctgtgc gcccgccgtg agcggccacg cagccgcgcc gccgagcagc gtgatgacgt
     2821 cgcgtcgctt cattcgatca cctcgtcggc gcgggcgagc agcgtgggcg gaatttcgag
     2881 gccaagcgcc ttggcggtcg tcagattgat gatgaggttg aacttggttg gctgctcgac
     2941 tggaaggtcg gcgggcttcg ccccgcgcag aatcttgtcg actaactcgg cggcgcggcg
     3001 gaagtgatcc ggaaggtttg ctccataaga tatcagaccc cccgcttcaa cgtactcccg
     3061 agcgccgtgc atagtcggca atcgcgcgcc caccgccaaa atgttgatgc gagtccgatt
     3121 ggtgttcatg agcgggtcgg gacaaagata aagtgcatcc gcgcggccct tgagggtctc
     3181 gaaggcaggt acgatatcct ccgctcgccg gatttcggat gtagtgatct caaggccgag
     3241 cgcgcgggcc gttgcctgga cctcgcgcat ttccaacacg gaggcgggat tgcctgcatt
     3301 ggccatgatt gccaaccggc gaagaccggg tacgacctcg cgcaggaatt cgagcttctt
     3361 gccactagtg tcagatatct ggttcgccag accggtgacg ttgccgcccg gtcgcgccag
     3421 actcgcgacc aggccggt
//
LOCUS       DATLJT010000270         5867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_474092,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000270 DATLJT010000000
VERSION     DATLJT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474092"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VM910_06125"
     CDS             <1..818
                     /locus_tag="VM910_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62154.1"
                     /translation="QMLTESFGQDFFVENVSGASGVRGALMVAAAPGDGYTLMVATGD
                     LAIAPVMSAKVGYDALKNFVPISIISRSPSVLLLHPSVPAKTVQEFAALARADPAKYS
                     YASMSLGQNLLNTERLFRLTLKLPIVRIPFPGAAPILSSTAAGHTLVAYIGLPPATPF
                     IKEGTLRALAVTSPTRSPIAPDVPTMAESGLAGQETELVIGLVAPARTPPSIVDLLAR
                     AMAEFVARPQTQERLAVFGFTAVGSTPEEFAAQIKSDVDRAAAVVRDAGIKVD"
     gene            complement(848..1957)
                     /locus_tag="VM910_06130"
     CDS             complement(848..1957)
                     /locus_tag="VM910_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HVI62155.1"
                     /translation="MITAHTDVVGSLLRPAALRKARDELAAGQLSRAQFKSIEDRAVD
                     AAVSLQEEAGLDIVTDGEMRRLSFQSPMTEAVDGFGPWDINAFLWGHWHGNGALGEQR
                     RDRPVNLGVVSRLVRKRHLAAEEFVYLRGRTRGVAKVTLPSPSLFLNFWSPERSQSAY
                     ASLEHFLADVVAILRGEVAELLRLGATYIQIDAPHYALLLAPETRSFYESRGWTLDQW
                     LSCGIELDNAVMAGFADVTFAIHLCRGNQESRWLIAGDYEPIARPIFRRIRATRLLLE
                     YDDDRSGSFAPLRLVPDDKTMVLGLISTKRPRMEAPEDLVRRIMEASRFVPLERLALS
                     PQCGFASSILGNNLSIEDERRKLERLCETARRIWG"
     gene            complement(2123..2896)
                     /locus_tag="VM910_06135"
     CDS             complement(2123..2896)
                     /locus_tag="VM910_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62156.1"
                     /translation="MSFFQRLLGRQPSYDHRRESIVAACIQSIERNPGQETHYLHLLN
                     SCEKAGELDAAIACLTKLVARNRHIDMPYRYLLVAHAKKGDFNNLTELVKHHYQNEKA
                     HQWFLVHAYDTRDWDSAIALLRQLVELFPRDEKWPRYLVAACGKKREVVAGASERTSL
                     TNDMHSEDVGAAGKYSEPEVPASKPSIPADARLTPQESTPKRDIEFETNLKLKQKQAE
                     IDAEERERKKYGPLRRPRALTPRQKFNSIKPTGSRDGYS"
     gene            3937..4350
                     /locus_tag="VM910_06140"
     CDS             3937..4350
                     /locus_tag="VM910_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62157.1"
                     /translation="MKNGVLRQRKGESAVAGLRIETPPSADYVLKLVNIRDGKEEMLI
                     YVHRGSTFETKIPLGAYKINGAYGETWYGEKYLFGPERTSFIRLVQKDGKSDQFTFYR
                     SGNRTKGYVVHGFDIHLTQQISGNLETQNIRAEEF"
     gene            complement(4384..5367)
                     /locus_tag="VM910_06145"
     CDS             complement(4384..5367)
                     /locus_tag="VM910_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HVI62158.1"
                     /translation="MVLGFVLKSPGTQAGPTDPLPAPRISVFGTIHPELFRLDAPLGS
                     GAPGERIRLASIEPQVGLFDPPTYRAPVLTDAAASASAPFEERFAAVEDHPVSFDKRF
                     ASTDDCPASFDKGLASAMQVLASSLRLPPDQGIEKKQAAAPAKPTVAKASVSVSPPRK
                     EGPTLEDDGRTAIYDITARVVYLPSGRRLEAHSGLGGYMDSPRHVHLRMRGATPPNVY
                     NLTLRERLFHGVRAIRLNPVDGSRMYGRAGILAHSYMLGANGQSNGCVSFSNYPEFLN
                     AYLRGEVTRLAVVESLPSPPYRLAAGQLPQPVKDMMKTADHQRQYAAAGDR"
BASE COUNT         1148 a         1784 c         1770 g         1165 t
ORIGIN      
        1 cccaaatgct cacggaatcg tttggccagg acttctttgt ggagaacgtg agcggggcaa
       61 gcggcgtgcg cggcgcgctg atggtcgccg ccgcgccggg cgatggctat acgttgatgg
      121 ttgcgacggg cgatctcgcg atcgcgcccg tgatgtcggc caaggttggc tacgatgcgc
      181 tcaagaattt cgtaccaatc agcatcattt cacgctcgcc ctcggtgctt ctgctgcacc
      241 cgtcggtgcc ggcaaagact gtgcaggagt ttgcggcgct cgcgcgcgcg gaccctgcaa
      301 aatacagcta cgcgtcgatg agcctgggcc aaaatctctt gaacaccgaa aggctttttc
      361 gtctcacttt gaagcttcca attgtccgca tccccttccc gggggcggcg cccatcctca
      421 gttcgacggc ggccggccat accctcgtcg cctatatcgg gctgcctccg gcaaccccgt
      481 tcatcaagga aggcacgctc cgggcattgg cggtcaccag cccgacgcga tctcccatcg
      541 ccccggacgt gccgaccatg gcggagagcg gtcttgccgg ccaggaaacg gagctggtga
      601 tcggcctggt cgcaccggct cgcacgccgc cgtcaatcgt ggaccttttg gcgcgcgcga
      661 tggcggagtt cgttgcccgc ccgcaaacgc aggaacgcct cgcggtgttc gggtttactg
      721 cagtcggcag tacgccggag gaatttgccg cccaaatcaa atccgatgtc gatcgagcgg
      781 ccgcggtcgt tcgggacgca gggatcaaag tcgattgaac cgcgtcgacg cgtgccggag
      841 gaggaagcta tccccatatc cgtcgcgcag tctcgcacag cctctcgagc ttgcgtctct
      901 cgtcctcgat ggacaggttg tttccaagga tcgaagaggc aaagccgcac tgagggctga
      961 gcgccaagcg ttccagcggc acaaaacggc ttgcttccat gatgcggcga acgaggtctt
     1021 ccggcgcctc cattcgcggc cgcttggtgc tgatcaatcc gaggaccatc gtcttgtcat
     1081 cggggacgag ccgaagcggt gcaaacgagc ccgaccgatc atcgtcgtat tcgagcagaa
     1141 gccgcgttgc acggatgcgc cggaaaatcg gccgggcaat cggctcatag tcgcccgcga
     1201 tgagccaacg gctttcttgg ttgccacgac agagatggat ggcgaaggtc acatcggcaa
     1261 atccggccat gacggcgttg tcgagctcga ttccgcaact caaccactga tcgagcgtcc
     1321 agccgcgcga ttcataaaag cttcgggtct caggcgccag cagcagggca tagtgcggcg
     1381 cgtcgatctg aatataggtg gcgccgaggc gaagcagctc cgcgacctcg ccacgcagga
     1441 tcgcgacgac gtccgcgagg aagtgctcga ggcttgcata cgccgattgg gaacgctccg
     1501 gcgaccagaa attcaagaac aggctggggc tgggcagggt tacctttgcg acgccgcgcg
     1561 tgcgaccgcg cagatagacg aactcctcgg cggcaagatg ccgtttgcgc acgagccggc
     1621 tgaccacgcc gagattgacc ggccggtcgc ggcgttgctc cccgagggcg ccgttgccat
     1681 gccaatggcc ccacaagaat gcattgatgt cccaggggcc gaagccgtcg accgcctcgg
     1741 tcatcgggct ctggaacgaa agccggcgca tttcgccgtc ggtcacgatg tcgagccctg
     1801 cctcctcctg gagagagacg gcggcatcga cggcgcgatc ctctatggat ttaaattgcg
     1861 cgcgactaag ttgcccggcc gccaactcgt cccgtgcttt gcgcaacgca gccggccgca
     1921 gaagactgcc gacaacatcg gtatgagccg taatcatcgc gggctcgggg caagatttgc
     1981 ttattctcag ctctttcttt ttgacccatt accgacatgg tactgaccag cgggcgcaac
     2041 ctggtaggct tgatgcacac ggggcggcgg ccgcaagcga atttttcgac ggggcaaacg
     2101 gatcgcgttc ggtgtggctt ttctaactgt atccgtcacg cgacccggtg ggctttattg
     2161 aattgaattt ctgacgagga gtaagggctc tcggtcgtcg caggggccca tattttttcc
     2221 gctcgcgctc ctccgcatca atctctgcct gtttttgctt cagcttaaga tttgtttcga
     2281 actctatatc tcttttgggg gtgctctcct gcggagtcag gcgggcgtcc gcgggaatac
     2341 tcggtttgct ggctggaact tccggttcgc tgtacttccc ggccgcccca acatcttcgg
     2401 aatgcatgtc attcgtcaag cttgtccgct ccgaagcgcc ggccacgacc tccctctttt
     2461 ttccgcaggc tgcgaccaaa tagcggggcc acttttcgtc ccttggaaaa agctcgacca
     2521 actggcgcag gagtgcgatg gcgctgtccc aatcgcgagt gtcgtaggcg tgcacaagaa
     2581 accactggtg cgccttctca ttctggtaat gatgcttcac caactccgtc agattattga
     2641 aatcgccctt ttttgcatgc gcgacgagta ggtatctgta tggcatatca atgtgccgat
     2701 tgcgggcaac tagtttggtc aggcatgcga tcgcggcatc aagctcgcct gctttttcgc
     2761 aagaattgag tagatgcagg taatgcgtct cctgacctgg attgcgctcg attgattgga
     2821 tgcaagcggc tacaatgctt tctcgcctgt gatcatagct gggttggcgg ccaagcagac
     2881 gctgaaagaa ggacatggcc ccacctcgtt tggattcgac gcgctctcgc gcacggtcat
     2941 actgattgcg tcgccccgcc gcgggagagc ctgcaccgtg gggcagagcg tgggagagtc
     3001 gtcaacgaaa atcttaatct agcaagtggc gcatacgtcc gcattgtaaa aatatccgga
     3061 agggatgttg acctttctct gatactttga cgctcccctt gcatctggtt tctatgctga
     3121 tgcattgcgc cgaatttcgt gagagcgctc gactgaaaga cgtttacaat ccgagcagcg
     3181 ggcataagat cgactgctaa tccggccact cctcagcagc ggacacatta caaaaaaggg
     3241 cacgcatacc ttttactaaa gacgtacgtg cagcggacta gcatctagcc acacacagcc
     3301 tagcgcgtcg tttcccaaaa atttggtgaa agcttcacct attaccctgg tcgatgttct
     3361 tcggcattgg gcatcagaat cgggagaata ggacaacatc catcgatggc cgcaaatcac
     3421 gttgtcattt gcgcgtgcgg ccaatcgaac cgcataaagg accaacgcgg aaaacgtggt
     3481 tggtttagct gcggtaagtg cggacggcgg ctcaagattt ctcccaatac gacgtccaca
     3541 ggattgacga ggtggatcgg atggggattg cttctcgtca tggccggtgg agccatggcg
     3601 ggaatctatt tggcgaagcc acccggcgcc caaatgacca atccgacagt acagacgacc
     3661 cttccggcaa atccggccgt atatggtgag acactccggc gcgaacctga ttctaatgtg
     3721 tctcctcggc agtccggtcc agcaatccct caagttcctg gtcctgctcc gtttccccgt
     3781 gaaccgactt ggatagaagc tacgcgacca gacccgaacc tcggccgccc cgcggtcggg
     3841 gacgcagtac cggaccccgg caggcatgcg ccgtcaccgc gcgatcaggt cgcacagcca
     3901 acgcagcctc agacacccca gctctcgccg attccagtga aaaacggcgt gctgcggcag
     3961 agaaagggcg agagcgccgt tgctggactg aggatcgaga cgcctccatc ggccgactat
     4021 gttctgaaac ttgtcaatat ccgcgatggc aaggaagaaa tgctaatcta cgtccatcgc
     4081 ggttcaacct ttgaaaccaa gattccactc ggggcttaca agatcaatgg cgcgtatggc
     4141 gaaacatggt atggcgaaaa gtatctgttc ggccctgagc ggacgtcttt tatccgacta
     4201 gtccagaaag atgggaaaag cgatcaattc acgttctatc gtagcggcaa tcgaacaaag
     4261 ggctatgtag ttcacggatt tgatattcat ttgacgcaac aaatcagcgg caatcttgaa
     4321 acgcagaaca ttcgagcaga ggagttctag cagctctccg ctggcgcgtc cttgtgccgg
     4381 gcgtcagcga tcgccggcgg cggcgtactg acgctggtgg tccgccgtct tcatcatgtc
     4441 tttgaccggc tgcggcagct gtcccgcggc gagcctgtag ggcgggcttg gaagactctc
     4501 gaccacggcg aggcgcgtga cttcgcctct gaggtaggcg ttcaggaact cgggatagtt
     4561 gctgaacgac acgcatccgt tcgactggcc gttcgcgccc agcatgtagg agtgggcgag
     4621 gatgccggcg cggccgtaca tcctgctgcc gtcgacggga ttgaggcgga tggcgcgaac
     4681 gccgtgaaac aatcgctcgc gcagagtgag attgtacacg ttgggcggcg tcgccccccg
     4741 catccgcagg tgcacgtggc gcggactatc catgtagccg ccgaggccgg aatgggcctc
     4801 cagccggcgg ccgctcggca gatagactac acgcgcggtg atgtcgtaga tcgcggtgcg
     4861 gccgtcgtcc tcgagcgtcg ggccctcctt gcgcggtggg ctcacgctga ccgacgcttt
     4921 ggcaacggtc ggtttcgccg gcgcggcggc ttgcttcttc tcgatgccct gatccggcgg
     4981 caagcgcaga ctgctcgcca gcacctgcat ggccgaggcg agacccttgt cgaacgaggc
     5041 cggacaatcg tcggtcgacg cgaatcgttt gtcgaatgag acgggatgat cttccaccgc
     5101 ggcgaagcgc tcctcgaacg gcgcgcttgc cgacgcggcg gcgtcggtga gcaccggagc
     5161 ccgataggtc gggggatcga acaatccgac ctgcggctcg atgctcgcca gccgtatgcg
     5221 ctcgcccggg gcccctgatc ccagcggcgc gtcgagccgg aacagctcag ggtggattgt
     5281 cccgaaaacc gaaatgcgcg gcgcgggcaa tgggtccgtc ggccctgcct gcgttcccgg
     5341 cgatttgagg acgaacccca gaaccatcgc ggcgattcca agcggaatcg cgcggacccc
     5401 ccattgaacc aaaacgtccc cccgctccca acccgcgtta cctgtggcgc agcggccaac
     5461 ggcaacgcac gtcccccgac gcaggtgcaa gcaaacatgc cgattgcggc aacaatcgtt
     5521 ccgagattgc ggcaagaatc gttcggactg agccgctccg cgcgccaatt cctcaccgcc
     5581 ccggcggcgt taccaacggt taactgcgtt cacaaatcca caaatcggct tgccgtcttc
     5641 ccaccggcgg ggctgccgcg gcgtccacta gtcggcgcga ggggtgggcg gaccggccgg
     5701 ttgcggcgcg ggctatgcgc cggcccgcgg cagcactaac atctggcgta actcgatcag
     5761 gctgaggaga gcgtttccgt gacggcaatc gcactcaagg acatcaccat tcatccggtc
     5821 gtcgagcagc aggcgccgtt cttcgacgtc ttcgaattct tcccgac
//
LOCUS       DATLJT010000271         5193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1622716,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000271 DATLJT010000000
VERSION     DATLJT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622716"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            888..1364
                     /locus_tag="VM910_06150"
     CDS             888..1364
                     /locus_tag="VM910_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62159.1"
                     /translation="MARNKWITKHWARISKLSDDALKSLFAVESLEDAPEKYREHKLA
                     LMKLLVIARGRLAAPKIDDGFPKANPRGWFQFYVLEAWIELGGQLRISRHPTTGEIRG
                     PLARYVAAVTGPVPGYRGSLESLPDILARQKSMMLKKARYNATTKLKDVEEMPHEP"
     gene            1697..2167
                     /locus_tag="VM910_06155"
     CDS             1697..2167
                     /locus_tag="VM910_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62160.1"
                     /translation="MSASPQKARYEQLLGSTALDWRALAADAWKGPSWANAAREYHEA
                     RQDSAGKPRPPDPQLVALLDPKVSLEQAQRRLFEHRRDGAAAFTVEALMFGLRDGIHA
                     LPTNPERLRRLSELSADQLKDVCARLQYLKPKIARPWTADEVTTLVRIWGKSHE"
     gene            2388..3467
                     /locus_tag="VM910_06160"
     CDS             2388..3467
                     /locus_tag="VM910_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HVI62161.1"
                     /translation="MKAFAYDEMQRTTMLMQKEGRPIEPRPVEDADVFRLQEFLQRAG
                     LKNLGTETVHNAVEQRGRECRFHPVRDYLEILKWDGTPRVENWLVRYLGAPETPYIQA
                     VGRMFLISMVARIFEPGCKADYMLVLEGPQAALKSTTCRTLGGAYFSDNLPDITVGKD
                     ASQHLRGKWLIEVSEMHAMGRAETTLLKAFITRQIEQYRPSYGRREVFEPRQCVFIGT
                     TNKQAYLRDETGGRRFWPVSTSSIDIAALKRDRHQIFAEAVRMYRSGVLWWPDRAFEQ
                     QHITIEQEARFEQDAWEENIEKYLVCAAQKVTVGQVAREALAIENRHLGRAEQNRIAA
                     ILERLGWQRGRRQKNARWWARGGDR"
     gene            3948..5015
                     /locus_tag="VM910_06165"
     CDS             3948..5015
                     /locus_tag="VM910_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI62162.1"
                     /translation="MGPPLMRRPPQYVNGFIDRYGKARWYFRRAGFKKVPLPGLPWSP
                     EFMAAYEHALAGQPLQIGSARTSPGTVGALAASYFNSSDFRSLKPSSQAIYRGVIDRF
                     CIQYGDNRVAGLKREHVVKLMAARAEKPVAANNLRKVLRAMMKHAVEIGLRADDPTRD
                     VKAIRVKSNGFHSWNEDEIAQFEKRHPIGSLGRLAFALLLYTGQRRSDVVRMGPQHVR
                     DGVLGVRQQKTGIELAIPVHSTLADVIAETPTYHLTFLTNQFGRPFTAGYFGQWFREQ
                     CDMAGLRHCSAHGLRKAAARRLAEAGCSTHEIAAITGHASLKEVARYTEAADRKRLAQ
                     SAMAKVRTSSDKRDEEFVKKG"
     gene            complement(5054..5193)
                     /locus_tag="VM910_06170"
                     /pseudo
     CDS             complement(5054..5193)
                     /locus_tag="VM910_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT         1145 a         1513 c         1536 g          999 t
ORIGIN      
        1 tggcgacatg atccgcgagc atatttattt tcctatacct tggatcggac gtcacctccg
       61 ccccgatcca gtcaggaagc gcgaattcct ggccagcgtc agctagttca atctccgcca
      121 ataccacgcc atccagaagt ccttcgtaga cgtcgacgtg ccaattgttg ccctcatgcg
      181 atacgaagtg tcgaacctta tcaaggacat taccgtcaca cattgtgcgc aacatttcct
      241 cggcgtcaga gtagggaatg gcgtactcga actcagtgcg aaccgggccg cgccttcgac
      301 ttttgagcgc tatcgttgct acgtcgtttg cgatacgcac cctagcttta tggcctttgt
      361 tgttcgctat cagcccatca cgaatccgga cacttcagat gacggcggtt ttccagccct
      421 cgttagcaac taaaaatttg cgctcaattt ctaggctcat gtctgggtag tccgctaggc
      481 tacgaagcac ctgcatgatt ggatggaggg agcattggcg gcaggccgag cagctcgcaa
      541 atcaccgaga ggagtaggcc gccggcatca ccgagggcgc tatcttccgt cgcgttctgg
      601 gaagagcatg agaagttaga aggccggagc agcgatggaa gcgccactcc ggccacacca
      661 caaagacagg ttggagctgt cgtcatggat taccacgacc tgaatgcgcc agatgcgagg
      721 caagtcgccg aggctgttag gtttgattac accgaggaag attggattaa aatcgaaaaa
      781 tcagttcggc acttgcggcc tacccaagcg gacctcgagg acctccgtac gcgattgcta
      841 atttacatcc gcgttatctg tttgccgagc taaatcggcc aggaaaggtg gccagaaaca
      901 agtggatcac aaaacactgg gcccgaattt caaagctatc tgacgatgca ctcaaatcgc
      961 tgttcgccgt cgagagcctt gaggacgccc ccgagaagta tcgggaacac aagcttgcac
     1021 tgatgaagct cctcgtcatc gctcgaggcc gtctagctgc gccgaaaatt gacgacggat
     1081 ttccgaaagc caatccgcgc gggtggttcc agttttatgt gttggaagca tggattgaat
     1141 taggtggtca gctgcgaatt tctcggcatc cgaccacggg agaaatcagg gggcctctgg
     1201 caaggtacgt tgccgccgtt accgggcctg ttccggggta tcgaggatct ctagaaagct
     1261 tgcccgatat tttagcccgg caaaagtcga tgatgctcaa gaaagcccgc tacaacgcga
     1321 ccacgaaatt aaaggacgtt gaagaaatgc cccacgagcc ataatggggg accggctcac
     1381 gttttgccgt tcacaaatcc caattaaatt gggaacgcta caaccaaaaa tttccggcac
     1441 ttccctattt tgtcgagagt ttcgaatgcg cgtatccacg ctgcacattg tcagcgaggt
     1501 gcaccgtcat gcgagacgat tcgatcgccc ccgttcacct gcgtctcggg aagcttttca
     1561 actcagttga agattggcgc agggtgcagc cgcgaatccc cacacgcgct gatgcaatca
     1621 aagatttgct gaaaatcgct ctcgatgccg agcggcagcg cctcgagcag tcagtgccgc
     1681 cagggcgagg ctagccatga gcgcctcccc ccagaaggcg cgttatgagc agttgcttgg
     1741 gtcgaccgcg ctcgattggc gggccctcgc cgccgacgcc tggaaggggc cgtcatgggc
     1801 gaatgccgcg cgcgagtacc acgaagcgcg gcaggacagc gctggcaagc cgcgaccgcc
     1861 cgatccgcag ctcgtcgccc tcctcgatcc aaaggtttcg ctcgagcaag cccagcgcag
     1921 gctgttcgag catcgccgag atggcgcggc ggctttcacg gtcgaggcac taatgttcgg
     1981 gctgcgcgat ggcatccatg cgttgccgac aaatcctgag aggctgcggc ggctctcaga
     2041 gttgagcgct gaccaactca aggacgtctg cgcgcgcctg cagtacctca agcccaaaat
     2101 tgcccggccg tggacggcgg acgaagtcac aacgttagtc aggatctggg ggaagagcca
     2161 tgagtaagct ctcctccgaa gaggtccaac ggctaatcga cgaagcgctc gagcatgcct
     2221 ccaagcgcaa gcgcaagcct gcggcgggca acggcaaggc aaaagcgact gatccctcaa
     2281 aaccggaaaa accggcctgg ctgagcaaat ccatcaaggg caaagcacaa gggccgctct
     2341 caatccttgc caatgtgatg gccgcgctcc ggcacgaccc atccctcgtg aaggcgtttg
     2401 cctacgacga aatgcagcgc accacgatgc tcatgcaaaa ggaagggcgg ccaatcgagc
     2461 ctcggccggt ggaggacgcc gacgtctttc ggctccaaga atttctccag cgtgcgggcc
     2521 tgaaaaacct cggcaccgag actgtgcaca atgccgtgga gcagcgcggc cgcgaatgca
     2581 gatttcatcc cgtgcgggac tatctcgaaa tcctgaaatg ggacggcaca cctcgcgttg
     2641 aaaattggct tgtgcgttac ctcggcgcgc cagaaacccc gtacatccaa gccgtcggac
     2701 gaatgttttt gatttcaatg gtcgcgcgca tttttgaacc cggatgcaaa gccgactaca
     2761 tgctggtgct cgaggggcct caggcagcct tgaagtcaac gacctgcagg accctcggcg
     2821 gcgcgtactt ttcagataac ctcccggaca tcacggttgg taaggacgct tctcagcact
     2881 tgcgcggaaa atggctaatc gaggtctccg agatgcatgc gatgggcagg gccgagacaa
     2941 ccctgctcaa agcgttcatc acgcggcaaa tcgagcagta ccgccccagc tatggtcgcc
     3001 gggaggtctt cgagcctcgt caatgtgtct tcatcggcac gaccaacaaa caggcctacc
     3061 tgagggacga gacgggcggc cgtcgcttct ggccggtatc gacaagcagc atagacatcg
     3121 ctgcgctcaa gcgtgatcgc catcagatat ttgccgaggc ggtgagaatg taccgcagtg
     3181 gcgtcctctg gtggcccgac agagccttcg agcagcagca tatcacgatc gagcaggagg
     3241 ctcgatttga gcaagatgcg tgggaagaaa acattgagaa atatctcgtt tgcgcggccc
     3301 agaaggtgac ggtcggtcag gtcgcccgcg aagccttggc tatcgagaac cggcacttag
     3361 gacgcgcgga gcaaaaccga attgctgcga tcttggaaag gcttggttgg caacgcggca
     3421 ggagacagaa gaacgcaagg tggtgggcaa gaggtggtga ccgatgaccg acggtgaccg
     3481 acttcctatt atgagcatca ggaatgtttc ccgtagggtt ctatatagag aaccacgtca
     3541 ccttcgtcac cttggtcacc gtcggtcacc cgctgctgcc tggccaagaa aatcagcgca
     3601 ccgctgcgat cactgcggca gccagttcgg ggacatgggc ccctgggatt ggccgcccga
     3661 ctgccccacc cgccaaattt tgctacacca ccactgcgaa cagccttggt gggactgcgg
     3721 cgggcagccg gagggcgttc gatgacgggc gataagaaca attcccgccc gccgtctgcg
     3781 ttccggcagc gcgacgttac ccgagcaatt cggggcgctg tcgcggcggg tgtggacatt
     3841 gcacgcgtcg aggtcacgaa cgccgggacg atcgttatag tccctgccgc cgcggccacc
     3901 accgccgggg ccgccgctca gaacggtgag agcgcggaga cgaatgaatg ggaccgcctc
     3961 taatgcggcg accgccgcaa tatgttaacg gcttcatcga ccgttacggt aaggcgcgct
     4021 ggtactttcg tcgtgcgggg ttcaagaagg ttcccctccc cggcctgccg tggtcacccg
     4081 aatttatggc cgcctatgag cacgcgctcg ccggacagcc gctgcaaatc ggtagtgcgc
     4141 ggacaagtcc cggcacggtc ggcgcccttg cggccagcta tttcaactcg tccgatttcc
     4201 gttcgttgaa accatcctcg caagcaatct atcgcggcgt cattgatcgt ttctgcatcc
     4261 aatacggcga caatcgtgtc gcgggcctga aacgtgagca tgttgtgaag ctgatggccg
     4321 cgcgggcaga aaagcccgta gccgcgaata acctacgcaa ggtgcttcgc gccatgatga
     4381 agcatgccgt cgagatcggc ttacgggccg acgatccgac gcgcgacgtg aaggcaatcc
     4441 gcgtcaaatc caacggtttc catagctgga acgaagatga gatcgcgcaa ttcgagaagc
     4501 gtcatccgat agggtctctt ggacgcttgg cgttcgccct tttgctctac accggccagc
     4561 ggcgatcaga cgttgtgcgg atggggccgc agcatgttcg cgacggggta ttgggtgtcc
     4621 gccaacagaa aacgggcatc gagcttgcga tccctgttca ctcaacgctt gccgatgtta
     4681 tcgccgagac gccgacttat catcttacct tcctgactaa tcagttcggt cgcccgttca
     4741 cggcgggcta cttcggtcaa tggttccgtg agcagtgtga catggcgggc ttgcgtcatt
     4801 gcagcgccca cgggctccgt aaggccgctg cgcgccgctt ggccgaggcg ggatgcagca
     4861 ctcatgagat cgctgcgatc accggtcacg ccagcttgaa ggaggttgcg cgctacaccg
     4921 aggccgccga ccggaagcgg ctggcacaat cggccatggc caaagtgaga acatcgagcg
     4981 acaaacgcga tgaagagttt gtcaaaaagg gctaaaaagc cttacaaatc aacgcggaat
     5041 tttgtccctg ctaaaccgga gcggtttgga cgatggtggc gagctgggtg gggccattgg
     5101 cgcccaccag ttcgcccagc gacacctggc gcgcagtgac gatgccgtcg aagggagcct
     5161 ttagctcggt gtaaccgagg ttgagctcgg cct
//
LOCUS       DATLJT010000272         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3001246,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000272 DATLJT010000000
VERSION     DATLJT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3001246"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VM910_06175"
     CDS             <1..220
                     /locus_tag="VM910_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083558.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI62163.1"
                     /translation="PYLETPLMELTNKPPTENSRGLRLGNMVQLRDIWAEEIEQALAG
                     KKTAKQALDSAVERGNTMLRQFEKTAVR"
     gene            272..1153
                     /gene="ugpA"
                     /locus_tag="VM910_06180"
     CDS             272..1153
                     /gene="ugpA"
                     /locus_tag="VM910_06180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HVI62164.1"
                     /translation="MQKQAVFQSRLLPYLLVAPQLAVVLIFFYWPAAQAVLQSFLVQD
                     AFGLSTSFVWFDNYRELLSEPDYFAAIVRTFVFSFAIALSSLSLALLLAVMADKPLRG
                     GTIYRTLLIWPYAVAPPVVGVLWVFMLNPSLGVLSHGLRTIGIDWNPLLNGNQAALLI
                     VLAAAWKQISYNFLFFLAGLQSIPKSVLEAAAIDGARPMRRFWTVTFPLLSPTIFFLL
                     VVNIVYAFFETFGIIDTMTRGGPAKATETLVYKVYQDGLLGGNLGGSAAQSVILMAIV
                     ILLTGVQFRFVERKVTY"
     gene            1153..2001
                     /gene="ugpE"
                     /locus_tag="VM910_06185"
     CDS             1153..2001
                     /gene="ugpE"
                     /locus_tag="VM910_06185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HVI62165.1"
                     /translation="MVEHEGIRRYVAHVILWIAIAIVAFPVYLAFIASTQDNAAIANG
                     QMSLLPGDHFLETYYQTIFVGTSGSTREPVGTMMFNSLVMAMLIAVGKIAISIISAYA
                     IVYFRFPFRMTIFWLIFITLMLPVEVRIYPTYKIVADLHLLDSYAGLSLPLIASATAT
                     LLFRQFFMTVPDELLEASRIDGAGPFRFFWDTLLPLSRTNMAALFVILFILGWNQYLW
                     PLLITTRDDMQTIQIGIRKMITTTDALTEWPIVMATAVLAMLPPVFVVLVMQKLFVRG
                     LVETEK"
     gene            2004..3092
                     /locus_tag="VM910_06190"
     CDS             2004..3092
                     /locus_tag="VM910_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HVI62166.1"
                     /translation="MANVALRNVRKAYPNGFEAIKGVSFTVGDGQFCVLVGPSGCGKS
                     TLLRMVAGLETVTAGEIDIGGRVVNQIEPAERDIAMVFQNYALYPHMSVYNNMAYGLR
                     NRGMARDEIDRRVREAARILELTAMLDRKPRQLSGGQRQRVAMGRAIVRQPKVFLFDE
                     PLSNLDAKLRIAMRVEIRKLQRRLNTTSIYVTHDQLEAMTLADVLVVMNGGRIEQIGN
                     PLDIYQKPATTFVASFIGAPPMNLISLRDSDIRLHTQPAEAGILGIRPEDLQMANGAV
                     PRDGIALELTVEAIERVGAETYVYGVRSQQGALPAVSSKPGELPPGEILVRIPGQSGP
                     KVGERIRASAPRDKLHVFSADGGQRIEL"
BASE COUNT          583 a         1035 c          998 g          704 t
ORIGIN      
        1 gccttacctc gaaacgccgc tgatggaatt gaccaacaaa ccgccgaccg agaattcgcg
       61 gggcttgcgg ctcggtaaca tggtgcagct tcgcgacatc tgggcggagg agatcgagca
      121 ggcgctcgcc ggcaagaaga ctgcgaagca ggcgctggat tcggcggtgg agcgcggcaa
      181 caccatgctt cgccaattcg agaaaacggc cgtacgctaa cgggagatga agcgggccgc
      241 accggcggcc cgcctcgcct cgggcagcat catgcaaaag caagcggtct tccaatcccg
      301 attgttgcca tatctcctgg tcgcgccgca gctcgcggtg gttctcatct tcttttactg
      361 gcctgcggcg caggcggtgc tgcaatcgtt cctggtccag gatgcgttcg ggctttcgac
      421 gagcttcgtc tggttcgaca attatcgtga gctgttgagc gagccggatt atttcgcagc
      481 gattgtcagg actttcgtct tttccttcgc gattgcgctg tcctcgctgt cgctcgcgct
      541 tctgctggcc gtcatggcgg acaagccgct gcgcggcggg accatctatc gcacgctcct
      601 gatctggcct tatgccgtgg cgccgccagt cgtcggcgtg ctgtgggtgt tcatgctcaa
      661 tccgtcgctc ggcgtgctct cgcacgggct gcggacgatc ggcatagact ggaatccact
      721 gctcaacggc aaccaggccg cgcttttgat cgtgctggcg gcggcctgga agcagatctc
      781 ctacaacttc ctgttcttcc ttgcagggct gcagagcatt ccgaaaagcg tgctggaggc
      841 ggcagcaatc gacggcgcgc ggccgatgag gcggttctgg acggtaacct ttccgctgct
      901 gtcgccaacc atcttcttcc tgctggtcgt caacatcgtc tacgccttct tcgaaacttt
      961 cggcattatc gacaccatga cccgcggagg acccgctaaa gccaccgaga ccctggtcta
     1021 taaggtctat caggacggtc tgctcggcgg caatctcggt ggctccgccg cacaatcggt
     1081 catcctcatg gccatcgtca ttctgttgac cggcgtccag ttccgcttcg ttgaacgcaa
     1141 ggtgacctac tgatggtcga gcatgagggg atcaggcgct atgtcgccca cgtcatcctc
     1201 tggatcgcga tcgcgatcgt cgcgttcccc gtctatctcg ccttcatcgc ctcgacccag
     1261 gacaatgccg ccatcgccaa cggccagatg tcgttgttgc ccggcgacca tttcctggag
     1321 acgtactacc agacgatctt tgtcggaacg agcggctcga cgcgcgaacc ggtcggcacg
     1381 atgatgttca attcgctcgt catggcgatg ctgattgcgg tcggcaagat cgcgatctcg
     1441 atcatctccg cttacgcgat cgtctatttc cgctttccct tccgcatgac gatcttctgg
     1501 ctgatcttca tcaccttgat gctgccggtc gaggtacgga tctatccgac ctacaaaatc
     1561 gtcgccgatc tgcacctgct cgacagttat gccggcctgt cgctgccctt gatcgcgtcc
     1621 gcgactgcga cgcttctgtt tcgccagttc ttcatgaccg tgcccgacga gttgctggag
     1681 gcctcgcgca tcgacggcgc gggtcctttc cggtttttct gggatacgct gctgccgctg
     1741 tcgcgcacca atatggcggc gctgttcgtg atcctgttca tccttggctg gaatcaatat
     1801 ctctggccgc ttctgatcac cacgcgcgac gacatgcaga ccatccagat cggcatccgc
     1861 aagatgatca ccaccaccga cgctctgacc gaatggccga tcgtgatggc gaccgcggtg
     1921 ctcgccatgc tgccgccggt cttcgtggtc ctcgtgatgc aaaagctgtt cgtgcgcggc
     1981 ctggtcgaga cggagaagtg aaaatggcca acgtcgctct tcgtaacgtc cgcaaggctt
     2041 atcccaacgg cttcgaagcg atcaaaggcg tcagcttcac tgtcggcgac gggcagtttt
     2101 gcgtcctggt cggcccctcc ggctgcggca agtccacgct gctcaggatg gtcgcggggc
     2161 tggagaccgt caccgcgggc gagatcgaca tcggcgggcg cgtcgtcaac cagatcgagc
     2221 cggcggagcg cgacatcgcc atggtgttcc agaattatgc gctctatccg cacatgagcg
     2281 tctacaacaa catggcctac ggcctgcgca accgcggcat ggccagggac gagatcgaca
     2341 ggcgcgtgcg ggaggcagcc cgaatcctcg agctgaccgc gatgctcgac cgcaagccgc
     2401 gccagctctc gggcgggcag cgccagcgtg tggcgatggg ccgcgccatc gtgcgccagc
     2461 cgaaggtctt tttgttcgac gagccgctgt cgaacctcga tgcgaagctc aggatcgcca
     2521 tgcgggtgga aatccgcaag ctgcaacgaa gactgaacac gacctcgatc tacgtcacgc
     2581 atgaccagtt ggaagcgatg acgctggccg acgttctggt ggtgatgaac ggcgggcgga
     2641 tcgagcagat cggcaatccg ctcgatatct accagaagcc ggcaacgacg ttcgttgcgt
     2701 ccttcatcgg tgcgccaccg atgaacctga tttccttgcg cgactcggac atccgcctgc
     2761 acacgcagcc ggcggaggcc ggcatcctcg gcatccgccc cgaagacctg cagatggcca
     2821 atggcgcggt tccccgtgac gggatcgcgc tggaactcac tgtcgaggca atcgagcggg
     2881 tgggtgcgga aacctatgtt tatggcgtgc gctcgcagca aggcgcactg ccggcggtca
     2941 gcagcaagcc cggcgagttg ccgcctggcg aaatcctggt tcggattccc ggccaaagcg
     3001 ggcccaaagt cggcgaacgc atccgtgcca gcgcgccgcg cgacaagctg catgttttca
     3061 gcgcagatgg agggcagcgg atcgagcttt gatcgtgccc gaggcttgat ctggttccga
     3121 ttaacgtttg agtgtggatt cgttctttcc ccaagacggt tgtccgaacg gttcccgacg
     3181 cgcttgaacc gatgggcgaa cgtgcccata ttgcttgcaa ggggtgccgc aagggccctg
     3241 aaacgtcaaa gtcgcttcga gaggactttg taaactatgt ctcgtgttcc ttcgctctcc
     3301 agtccgttcc ttttgggctt
//
LOCUS       DATLJT010000273         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2082570,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000273 DATLJT010000000
VERSION     DATLJT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082570"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VM910_06195"
     CDS             complement(<1..325)
                     /locus_tag="VM910_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62167.1"
                     /translation="MAAFGALQPAHHRDKKGTIDVKTKNKHQTSGGEVMELARETAKL
                     EQYWIVGDRSKPRKPPKNYNYVEELMHLQIELIKMQECVRHNRLKMCVIFEGRDAAGK
                     GGVIKR"
     gene            complement(662..2458)
                     /locus_tag="VM910_06200"
     CDS             complement(662..2458)
                     /locus_tag="VM910_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI62168.1"
                     /translation="MARFAKTATSRRDAAAFHVVLIKPTHYDDDGYPIQWVKAAIPSN
                     TLACLNGLAEDASRRQVLGPGVTIHVQTYDETNRRVTPDRIVRMINRAGGRALVGLVG
                     VQSNQFPRAVDLARPFLRAGLPVCIGGFHVSGSIAMLPEMPQEMRKAAALGISFFAGE
                     AERGRLDQVIRDAWDGMLQPLYNYMTDLPSLEGEPVPILPRKHVRRVSGQLSSLDLGR
                     GCPYQCSFCTIINVQGRKSRFRSPDDLERAIRENYRHGNRRFFITDDNFARNRHWQPL
                     FDRMIQLRVGEGLKIGFTIQVDTLCHRIPNFIEKAARAGVRRVFIGLENINPDNLLAA
                     KKRQNKITEYREMLQKWHEHGAITCAGYIIGFPGDTKQSILRDIEIIKRELPLDVLEL
                     FMLTPLPGSEDHKVLWQQRTWMDPDLNKYDLNNRVTHHPVMSDQDWDSAYRAAWESFY
                     SFEHMRTILRRAAAHPRGRPHTTLTTLLWFKLMAMFEAVHPLEGGAFRRKSRRDRRYG
                     LPVESPFVFYPRYAGEIAGKARQYWSVYRKARKILKEVFAAPDRWTYSDLAIAPPRSD
                     EYDRLDLYRATAGGEAALVRKRREDSLRTRVA"
BASE COUNT          538 a          807 c          848 g          556 t
ORIGIN      
        1 tgcgcttgat gaccccaccc ttgcccgctg catcccgccc ctcgaagatg acgcacatct
       61 tgagacggtt gtgcctcaca cactcctgca tcttgatcag ctcgatttgg aggtgcatga
      121 gttcctcgac gtagttgtaa ttcttcgggg gtttgcgtgg tttggatcgg tcgcctacga
      181 tccaatactg ctcgagtttt gcggtttccc ttgctaactc catcacttcg ccgccagagg
      241 tttggtgttt gttcttcgtc ttcacgtcga tcgtcccctt cttatcgcga tggtgcgcgg
      301 gctggagagc gccgaaagcg gccatctgca cacgcggtac cgagttggca gggtcgaccc
      361 gaagctgccg tcgccgcccg agagacatcg ggttgcccgt gacccattcg ggacatcacc
      421 tctcggcccg taggttcaag gaaaccgcca gccagatttc agatgaaccc gcggctacct
      481 tgagacattt tcatgtgcga aaaattgccc aggcttgacc aatggcgcgc tcacccggag
      541 cattagatat ggcgcggcgc gcattgctct ccagcacggc gcgcaggcta aatacaacat
      601 atcatctttc agtcgaacgg ctgcaggctg taacaaccag acgacgccaa aaatgttgcc
      661 ttcacgccac acgcgttcgt agcgagtcct cccgacgctt gcgaacgagc gcagcctcgc
      721 caccggcggt cgcgcgatag agatcgagcc ggtcatattc atcgctgcga ggcggagcga
      781 tcgcgagatc gctgtaggtc caccggtcag gtgcggcgaa gacttccttg aggatctttc
      841 gcgcctttcg gtaaaccgac caatactgcc gagcctttcc ggcgatttca ccggcataac
      901 gggggtagaa gacaaagggg ctttccacgg gcaagccata gcggcggtcc cgccgtgact
      961 tccggcgaaa ggccccacct tcgagcgggt gaaccgcttc gaacatcgcc atcagcttga
     1021 accatagcaa tgtggtcaac gtcgtgtgcg gtcgtccccg cggatgcgcc gccgcacgtc
     1081 gcaagatcgt gcgcatatgc tcgaaactgt aaaagctctc ccatgccgca cgataggcac
     1141 tgtcccagtc ctggtcggac atcaccggat gatgcgtcac ccggttgttg aggtcgtact
     1201 tgttgaggtc ggggtccatc caggtgcgtt gctgccacag taccttgtgg tcctcggagc
     1261 cgggaagcgg agtcagcatg aataactcga gcacgtcgag gggtaactcc cgcttgatga
     1321 tctcgatgtc gcgcaggata gactgcttcg tatcgccggg gaatccaatg atgtagccgg
     1381 cgcaggtgat ggcaccatgc tcatgccact tctgcagcat ctcgcggtat tcagtaatct
     1441 tgttctggcg ctttttagcc gcgagcagat tgtcgggatt gatgttctcc agaccgatga
     1501 agacgcggcg aacgccggcc cgcgcggctt tctcgatgaa attcgggata cggtggcata
     1561 gcgtgtccac ctgaatcgta aagccgatct tcaggccttc accgacccgc aattggatca
     1621 tccggtcgaa cagcggttgc caatgacgat tgcgggcgaa attatcgtcg gtgatgaaga
     1681 agcgccggtt gccgtgacgg tagttctccc ggatcgcgcg ctcgagatcg tcgggcgagc
     1741 gaaatcggct cttgcggccc tggacgttga tgatcgtgca gaaggagcac tggtaagggc
     1801 atccgcgacc gagatcgagg ctcgacaatt gcccggaaac tcggcggacg tgcttgcgcg
     1861 gcaggatcgg cacgggctct ccctccaatg agggaagatc cgtcatgtag ttatagagcg
     1921 gttgaagcat gccgtcccag gcatcccgaa tgacctgatc gagccggccg cgctctgcct
     1981 cgccggcaaa aaaggagatg ccgagtgccg ccgccttgcg catctcttgc ggcatctccg
     2041 gcagcatcgc gatcgagccc gagacatgaa atccgccaat gcaaaccggc aggccggcac
     2101 gcagaaacgg cctcgccaga tcgacggcgc gtggaaactg gttcgattga acgccgacta
     2161 gcccgaccag ggctcggccg ccggctcggt taatcatccg gacgatccgg tcgggcgtga
     2221 cgcggcggtt ggtctcatcg taggtctgga catggatcgt cacaccaggg ccgagcactt
     2281 ggcgccgact ggcgtcttcg gcgagaccgt tgaggcaggc cagggtgtta gacgggatcg
     2341 cggccttgac ccactggatt ggatacccat cgtcgtcgta atgcgtcggc ttgatcagca
     2401 ccacgtggaa cgcggcggca tcgcgtctgg acgtcgccgt cttggcgaat ctggccaaat
     2461 tgatcgcttt cagctgagcg gctggggccg gctcggtatc ccaggtgccg atttgaacaa
     2521 aggctataat cgggacttcc tgcaatcgcg gcaacacctc agcggagcgg tctcggagcg
     2581 ggggtgcgtg ggacaaggca aggtccggaa tggggcgtgt gtcacgaagg caccgtctga
     2641 gtgcgacggt gcggaactgt acgagagatg aaggagggcc cttcgagacc ggcagtcagg
     2701 tgcaggtctc aagccaccgc aagctgctgt cgtcaagagc gatggtggc
//
LOCUS       DATLJT010000274         3802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2312549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000274 DATLJT010000000
VERSION     DATLJT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1536)
                     /locus_tag="VM910_06205"
     CDS             complement(16..1536)
                     /locus_tag="VM910_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62169.1"
                     /translation="MLPTISRRVFVVGAAALPFLRIAKADARTPGILTFGLSSYPPSI
                     QPWANTGTAAGTVKLMIHRGLLSYDTKGALRPELAETWTRDGSTAWLFKLRHAVFHNG
                     EAVTSADVKWTLEQVAAEKSTAYFRSEMQGVDRIETPDERTVRVVMKQPTATLSQWLA
                     SYHLPIISRKSDATQPIGAGPFVLKTQERGVALEVEAFPKYYRPGLPKLKGIRAIAYA
                     DENLRVAALQAGDVDLIEYVPWQSMETISNDPKLRLDATDGPFMYLLFNGTKAPFNDA
                     RVRKAVAHAIKREDIVNAAFFGRGSPLTHLPIVDSSEFFNPDLKDGWKYDLTLAKKLL
                     ADAGYPEGFRCSFLSTAQYGMHKDSAEVVQQNLAAIGIKAELNLPDWATRVTLGNRGQ
                     YDLAMGGTAADNNDPDGLANLIDGSLSPSYVRSFGVRLPKIEELLKAGRAEFDPAKRK
                     TIYKEMESLAIAEAPVVGLAWRSQGYAMQRDVTGFKNLPGALSFNSGLTFEETAVG"
     gene            complement(1557..3209)
                     /locus_tag="VM910_06210"
     CDS             complement(1557..3209)
                     /locus_tag="VM910_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HVI62170.1"
                     /translation="MNVAVAAAPSPTLDPITVEVIGAALSSIVEETGEALIRASYSTN
                     IKERRDCSTALFDVQGCTLCQAEHIPIHLGSFIGIVPHVLKHHAIAEMRPGDVFIGND
                     PYAGGGTHLPDIVMAQPIFVDGTIIAWTVNLAHHADFADRGHAHIYQEGLRIPPIRLF
                     CAGELQQDVLDLVLLNCQVPRERLSDLRAQMAANRVGVQRVQALCAKYGVATVLAAGN
                     ALLDYAERKMRAGIAAIPDGIYRFTDVFDNAEIDGELPMSVEITVFGDAMKLHFVSPP
                     QVRAGINMTYTALLATVYYAVKSVVDPTILPNAGLAWPLTVTAAEGTVLNCVHPAAVN
                     GRVQTCQRVSDMIFGALARAVPERVMACTNSACSVTTFVGQRPDDGTIWVYLETMGGG
                     SGARPDRDGLDGVHVHTTNTSNLPVEALEPEYPLTVMRYELIAGSGGSGKFRGGMGLR
                     RAYRTNAQCQVRLDLSRLRSESWGLLGGLPGQRSRVICGPGVVFEKDSATLQAGQWFE
                     VITPGAGGYGPPIERDRAVVARDLAEGVIDAETARKVYGYES"
     gene            complement(3206..>3802)
                     /locus_tag="VM910_06215"
     CDS             complement(3206..>3802)
                     /locus_tag="VM910_06215"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVI62171.1"
                     /translation="LRADFAATRLAPLARETLHHIETAFAALDRQAEAWFAQEHIDAE
                     ARRLIRTVDMRYAGQNYELSVSVPEGPVTAATVDLLAQGFADAHERMYGFVAEGEPVQ
                     LVTFRVEAIGLVRKATLKAEPLASEDATNAIREHRHVWIAEEERSVPCPVYERDRLKA
                     GNNFVGPAIVEQMDATTFILPGMIARVDVFSNLILEAA"
BASE COUNT          689 a         1174 c         1226 g          713 t
ORIGIN      
        1 tagtagagca tcgtctcaac ctacggccgt ttcctcgaag gtgaggcccg aattgaagct
       61 aagcgcgccg ggcagattct tgaagcccgt cacatccctc tgcatggcat acccttggct
      121 gcgccacgca aggccgacga ctggcgcctc ggcgatcgcg agtgactcca tctctttgta
      181 gatcgtcttg cgcttggcag ggtcgaactc ggcccgtccc gccttgagaa gctcttcgat
      241 cttcggcagc cgcacgccga agctacgcac gtaggatggc gagagacttc cgtcgatcag
      301 gttcgcgagg ccatccggat cgttgttgtc ggcggcggtt ccccccatcg cgaggtcgta
      361 ctggcccctg tttccgagcg tcacgcgcgt tgcccagtca ggcagattta gttcggcctt
      421 gattccaatg gcggcgaggt tttgctgcac cacctcggcc gaatccttgt gcatcccgta
      481 ttgcgccgtc gacaggaaac tgcagcggaa gccttccggg tatccggcat ccgccaagag
      541 ctttttcgcc aatgtgagat cgtacttcca gccgtccttg agatccgggt tgaagaactc
      601 gcttgaatcc acgatcggca agtgcgtcaa cggtgagccg cggccgaaga aggccgcatt
      661 gacgatatcc tcgcgcttga ttgcatgggc gaccgccttg cgcacccgtg catcattgaa
      721 cggcgccttg gtgccattga aaaggagata catgaacggt ccgtcggtgg cgtcgagcct
      781 cagcttcgga tcgttcgaga tcgtctccat ggattgccac ggcacgtatt cgatcaaatc
      841 cacatcgcct gcttgcagcg ctgcgactcg taggttttcg tctgcgtagg cgatggcccg
      901 gatgcctttg agttttggca ggccgggtcg gtaatacttt gggaacgcct ccacctcgag
      961 ggcaacaccg cgctcctgtg tcttgagcac gaaaggcccg gcgccaatcg gttgggtggc
     1021 gtccgatttg cgtgagatga tcggcaggtg gtagctcgcg agccactgcg agagggtggc
     1081 agtcggctgc ttcatgacga cccgcaccgt gcgctcgtcc ggcgtctcga tccggtcgac
     1141 gccctgcatc tcggaacgga agtaggcggt cgatttctcg gcggcgacct gttcgagcgt
     1201 ccattttaca tcagcactgg tgacggcttc gccgttgtga aatacggcat ggcgtagctt
     1261 gaacagccag gcagtcgagc catcgcgcgt ccaggtctcg gcgagttcag ggcgcagcgc
     1321 gcccttggtg tcgtaggaga gaaggccgcg atggatcatc agcttgacag tgccggcggc
     1381 agtgccggtg ttggcccagg gctggatgct cggcggataa ctagacaggc cgaacgtgag
     1441 aatgccgggg gtgcgcgcgt cagcctttgc aatgcgcagg aacggcagcg ctgcggcgcc
     1501 gacgacgaac acgcgccggg agatggtcgg gagcatgggc attctcctca tcaacgtcag
     1561 ctttcgtaac cgtagacctt gcgggcggtc tcggcatcga tgacgccttc agcgaggtcg
     1621 cgggcgacca cggcgcggtc gcgttcgatg ggcggaccat aaccgccggc gccgggtgta
     1681 ataacttcga accactggcc ggcttgcagc gtcgcactgt ccttttcaaa caccacgccc
     1741 ggcccgcata tgacacggct gcgctggcct ggaagaccgc cgagcaggcc ccaggactcc
     1801 gaccgcagac gcgatagatc gagccgcacc tggcactggg cgttggtgcg gtacgcccgc
     1861 cgcaacccca tgccgccacg gaacttgccg gaccccccag agccggcgat cagctcgtag
     1921 cgcatcacgg tcaagggata ttctggctcg agcgcctcga ccggcaggtt cgacgtgttg
     1981 gtggtgtgca catgcacgcc gtcgagaccg tccctgtcgg gccgggcgcc ggagccaccg
     2041 cccatggtct cgaggtagac ccagatcgtc ccgtcgtctg ggcgctggcc gacgaatgtc
     2101 gtaactgagc aagcgctatt ggtgcacgcc atcacgcgct cgggcaccgc tcgggccaag
     2161 gcgccgaaaa tcatgtcgga gacgcgctga caggtctgga cgcggccgtt caccgccgcc
     2221 gggtggacgc agttgagcac ggtgccctcg gccgctgtca ccgtcagcgg ccaggcaagg
     2281 ccagcattcg gcagaatggt ggggtcgaca acggacttga ccgcgtaata gacggtggca
     2341 agtagcgccg tgtaggtcat gttgatgccg gcgcgcacct gcggtgggct gacgaaatgg
     2401 agtttcattg cgtcgccaaa aaccgtgatc tcaacgctca tcggcagctc gccgtcgatc
     2461 tctgcattgt cgaacacgtc ggtaaagcga tagatgccgt ccgggatggc ggcgattccg
     2521 gcccgcatct tgcgctcggc ataatcaagc agcgcgttgc cggccgcgag cacagtggca
     2581 accccgtact tcgcgcacag cgcctggacg cgctgcaccc cgactcggtt cgccgccatc
     2641 tgagcgcgta gatccgagag gcgctcgcgc ggcacttggc aattgagcag gacgaggtcg
     2701 agcacgtcct gctgcaattc gcctgcgcaa aagagacgga tcggcgggat gcgcagaccc
     2761 tcctggtaga tgtgggcgtg gccgcgatcg gcgaaatcgg catggtgagc gagattgacc
     2821 gtccacgcga tgatcgtgcc gtcgacgaag atcggctggg ccatcacaat gtcaggcagg
     2881 tgcgttccgc cgcccgcata gggatcgttg ccaatgaaca cgtcgccggg gcgcatctcc
     2941 gcgatcgcat gatgcttgag gacatgcggc acgatgccga tgaagctgcc gagatggatt
     3001 gggatgtgct ccgcctggca cagggtgcag ccttgcacat cgaataaagc ggtcgagcaa
     3061 tcccgcctct ccttgatatt tgtcgagtag gaggcacgga tcagcgcctc gccagtctcc
     3121 tcgacaatcg atgacaaggc ggcaccgatc acctcgacgg tgatcggatc aagcgtgggt
     3181 gacggcgcag ccgcaacagc gacgttcatg ccgcctccag gatcaggttc gagaaaacat
     3241 cgacacgcgc gatcataccg ggaaggatga atgtggttgc gtccatttgc tcgacgatcg
     3301 cggggccgac gaagttattg ccggccttga gcctatcgcg ctcgtagacc ggacagggca
     3361 cgctgcgctc ttcctcggca atccatacgt gtcggtgttc gcggatcgca ttcgtcgcgt
     3421 cctcgctagc gagcggctct gctttgagcg ttgccttccg gaccaggcca atggcctcaa
     3481 cgcggaaggt cacgagctgg accggctcgc cctccgcgac gaagccgtac atgcgctcgt
     3541 gtgcgtccgc aaaaccttgc gcgagcagat ccaccgtcgc ggcggtgacc gggccttcag
     3601 gcaccgatac cgaaagctca taattctggc cggcgtagcg catgtcgacg gtgcgtataa
     3661 ggcgccgcgc ctcggcgtcg atatgttcct gagcaaacca cgcctccgcc tgcctgtcga
     3721 gggcagcgaa tgcggtctcg atatgatgca gcgtctcgcg ggcgagcggc gcgagtcggg
     3781 tcgcggcaaa atcggcgcgc ag
//
LOCUS       DATLJT010000275         3379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1852664,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000275 DATLJT010000000
VERSION     DATLJT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852664"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VM910_06220"
     CDS             <1..934
                     /locus_tag="VM910_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62172.1"
                     /translation="FLFFNPGVFVPDGQPEGKWTLLQNILFRHHAGTANYDGSLYIRG
                     VVNQEIAGLTNEGSGKKPAKPSTHAALDPSAGTPVTLFNGGKEPPQHLGYESMVPKNI
                     KDTFHDWATEILGQGVHIHSKVIVLDPFGAHPVVMTGSHNLGLKASSKNDDNLMIVEG
                     NGPLAAAYAANIIVIYQAYRWNSYVEAHRQDPKVWHGLVDNDTWQNGYLDPSGNDWPE
                     LQFWMGLHQPASAAKAGPAQVPEHTPSAPSVAPPRKNKRPAKHPSVKKAAARKKNTAK
                     RSPGKKTTHRAASTRHSAKGRTASRKRSHTRRAA"
     gene            1172..2347
                     /locus_tag="VM910_06225"
     CDS             1172..2347
                     /locus_tag="VM910_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HVI62173.1"
                     /translation="MIVRQFINWIRTASAGERAEATRALARAWLISDLSADDRAAAEG
                     ALLMMLDDPSPLVRQAMAEVFARSAEAPAAIVQALSVDQPSVAVPVLEHSPLLIDADL
                     VDIVATGNSEMQCAIARRIRLPASVCAAIAEVGSAAAALELIENAYAELASFSWDRIV
                     ERHGHLAAIRESMLVLDDLPAATRAALVAKLSETLAQFVVARNWLSADRAGRIASEAR
                     DRSTVNIAARSRGDDMEGLVRHLRATSQLTAGLILRALLSGNLDLFSYALSELSEMPH
                     ARVAALLHDRGGASLNALLRRAGLPESTFAAFRVALEVCHEVGFADSAGTAARLRRRM
                     VERVLTHCETDNDATEALLILLRRFATESAREEARLFCDEMLAGAAVPPACQDLIAA"
     gene            complement(2564..3142)
                     /locus_tag="VM910_06230"
     CDS             complement(2564..3142)
                     /locus_tag="VM910_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVI62174.1"
                     /translation="MPKNKSGDVEIKTFATHKVITQPNPLRKVLRRVDDKDADDPVAR
                     AEKALAGLSGEFKTWMLTEADRLSAAHAAAQKSGFSREACDELFRAAHDIKGDAATFG
                     FPSAAAAAESLCRIIEHAPNLSQVPGELIAHHINAIQAMVHGHTRLDSSIVAAELSRR
                     LRKVADDYLVHVNRDRPEHLEAITAPSIVPGE"
     gene            complement(3161..>3379)
                     /locus_tag="VM910_06235"
     CDS             complement(3161..>3379)
                     /locus_tag="VM910_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62175.1"
                     /translation="EFLAKPISAKGLYQRILNVVANPRPFIKTKTYFGPDRRRNTNNT
                     YIGPERRSGGEVEVLQQPSLLDKARSSV"
BASE COUNT          588 a         1053 c         1125 g          613 t
ORIGIN      
        1 gttcctgttc ttcaatcccg gcgtgttcgt gcccgacggc cagccggagg gcaaatggac
       61 gctcttgcag aacatcctgt tccggcatca cgcgggcacc gccaattatg acggcagcct
      121 ctatattcgc ggcgttgtga accaggagat cgccggcctc accaacgagg gcagcgggaa
      181 aaagccggca aagccttcga cgcatgccgc gctcgacccg tcggcgggca cgccggtcac
      241 gctgttcaac ggcggcaagg agccgccgca gcaccttggc tacgaatcca tggtgcccaa
      301 gaacatcaag gatacgttcc acgactgggc gaccgaaatt ctcggccagg gcgttcacat
      361 ccacagcaag gtcatcgtgc tcgatccctt cggagcgcat ccggtggtca tgaccggctc
      421 gcacaatctg ggcctgaagg cctcgagcaa gaacgacgac aatctgatga tcgtggaggg
      481 taacggaccg cttgctgcgg cctatgccgc caacatcatc gtcatctacc aggcctatcg
      541 ctggaactcg tatgttgagg cccaccggca ggatccgaag gtgtggcacg ggctggtcga
      601 caacgacacc tggcagaacg gctatctcga tccgagcggc aatgactggc ccgagcttca
      661 gttctggatg ggcctgcatc agcctgcctc cgccgcgaag gcgggcccgg cccaggtgcc
      721 tgagcacacg ccctccgcac cgagcgtggc gccgccgcga aagaacaagc ggcccgcaaa
      781 acatccgtcc gtcaaaaaag ctgcggccag aaagaaaaac acggccaagc gatcgcccgg
      841 gaagaagaca acgcatcgtg cggcaagcac gaggcactcc gcgaagggga ggaccgcgag
      901 caggaagcgt tcgcacacgc gccgcgcggc ctgacacggc gacgtctcgg ggtctgacgg
      961 ccacgggcga ggcgggtcag ccccggtgtc gcctcgccgg ggccagacgg ctgctgaaac
     1021 agggctgtcc cgacggtgac cggcggccgg gcaccggcac ccttaacaaa acggcaagaa
     1081 atccagccgg ttatggtgaa cgctttgtta agcgtcatgg tttactttcc cttcttatcg
     1141 tccgtctctc gttgcgtaag ccgtgagcac aatgatcgtt cggcagttca tcaactggat
     1201 caggaccgcc tccgccggcg agcgggcaga ggccacgcgc gcgctggcgc gggcctggct
     1261 catttccgat ctttccgcag acgaccgtgc tgccgccgaa ggtgcgctgc tgatgatgct
     1321 cgacgacccc tcgccgctgg tgcggcaggc gatggccgag gtctttgcgc gcagcgcgga
     1381 agcgccggcc gcgatcgttc aggcgctgtc ggttgaccag ccgtcggtgg cggttcccgt
     1441 gctcgagcat tccccgctgc tgatcgatgc cgacctcgtc gacatcgtgg cgaccggcaa
     1501 tagcgagatg caatgcgcga tcgcccgccg tatccgcctg cctgcttcgg tctgtgctgc
     1561 aatcgccgag gtcggcagcg ctgctgcggc gctcgaattg atcgagaacg cctacgcgga
     1621 gcttgcgtcg ttctcctggg accggatcgt cgaacgtcac ggccatcttg cggcgatccg
     1681 ggaatccatg ttggtgctgg atgatctgcc cgccgcaacg cgcgcggcgc tggtggcgaa
     1741 attgtcggag acgctggcgc agttcgtcgt cgcacgcaat tggctcagcg ccgacagggc
     1801 agggcggatc gccagcgaag cgcgcgatcg ttccactgtg aatatcgcgg cccgttcgcg
     1861 cggcgacgat atggagggat tggtgcggca tttgcgtgcc acctcgcagt tgaccgcagg
     1921 gctgatcctg cgcgcgctgt tgtcgggaaa tctcgatctg ttcagttatg cgctgtcgga
     1981 actgtccgag atgccgcatg cgcgcgtcgc ggcgctgctg cacgatcgcg gcggtgcgag
     2041 cctcaacgcg ctgctccggc gcgccgggct gccggaatcg acgttcgcgg ctttccgcgt
     2101 ggctctggaa gtgtgccacg aggtcggctt tgccgacagc gccggcaccg ctgcgcggtt
     2161 gcgtcggcgc atggtggaac gggtgctcac gcactgcgag accgataacg atgcgaccga
     2221 agcgctgttg atcctgctca ggcgctttgc gaccgaatcc gcgcgcgaag aagcgcgcct
     2281 gttctgcgac gagatgcttg cgggagctgc cgttccgccg gcctgtcaag acctgatcgc
     2341 ggcctgattg ttattgctgg aacatgatcc ggaaaagtgg gcaccggttt tccctcgcga
     2401 caaacgcgga acgcgtttgc gcggagatca tgctctaaac cagagcgcga tggcgattcg
     2461 acatacagct atcgcgctcc aggagcgcaa gccacgacgc aatccggctc tactggattg
     2521 ggtcgcttcg caatggcgaa ggtccgcagc ttgacgtttg cagttattcc cccggcacga
     2581 tgctgggcgc ggtgatcgcc tcgagatgtt caggccggtc gcggttgaca tgcacaagat
     2641 agtcgtcggc gaccttgcgc agccgccggc tgagttcggc ggcaacgatc gagctgtcga
     2701 gccgggtgtg gccgtgcacc atcgcctgga tcgcgttgat atggtgagcg atcaattcgc
     2761 ccggcacctg gctgaggttg ggcgcgtgct cgatgatgcg gcacaggctt tcggcggcgg
     2821 ccgccgccga tggaaatccg aaggtcgcgg catcgccctt gatgtcgtgc gcggcgcgaa
     2881 acaactcgtc gcaggcctcg cgggagaagc ccgacttctg cgcagcagcg tgagccgccg
     2941 agaggcggtc ggcctccgtc agcatccagg tcttgaattc tccggacagg ccggcgagcg
     3001 ccttctccgc gcgtgccacc ggatcgtcgg catccttgtc gtcgacgcgg cgcagcacct
     3061 tgcgcagcgg attgggttgc gtaatgacct tatgggtggc gaaggtcttg atctcgacgt
     3121 cgccggactt gtttttgggc atggatcgct tccttgggcg ctagaccgac gaacgggcct
     3181 tgtcgagcag cgagggttgc tgcaggacct ccacctcgcc gccagaacgg cgctcgggac
     3241 cgatataggt gttgttggtg ttgcgccggc gatccgggcc gaaataggtc ttggtcttga
     3301 tgaacgggcg cggattggcg acgacgttca ggatgcgctg gtagagcccc ttggccgaga
     3361 tcggcttggc gaggaattc
//
LOCUS       DATLJT010000276         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2772382,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000276 DATLJT010000000
VERSION     DATLJT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772382"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(607..744)
                     /locus_tag="VM910_06240"
     CDS             complement(607..744)
                     /locus_tag="VM910_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62176.1"
                     /translation="MFTTTAVVLTVGIARWIEGSAQDVELVAYSSAVLIMLGIIADRL
                     I"
     gene            complement(755..1102)
                     /locus_tag="VM910_06245"
     CDS             complement(755..1102)
                     /locus_tag="VM910_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62177.1"
                     /translation="MTTLSLGQTARLTGRGKTILARAISGRFFTGSKEEGGHNMGGAE
                     SVRVYPFPAPVETVDELRTRLALAEERLSELKAMLEDIRRDRDAWREQAQTRLLPAPA
                     APMSWWRWLRTTG"
     gene            1645..>2609
                     /locus_tag="VM910_06250"
     CDS             1645..>2609
                     /locus_tag="VM910_06250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI62178.1"
                     /translation="MSSGRRIVICGGGAIGAAIAYFTSRGGARSTVIERHIVAGAASG
                     KSGGFLALDWCHGSPLDRLARRSFELHAELSTELGDPWGYRRLTTYGGYAAEDDTARG
                     AGGRPWLADGVAITSRLGSPETTALVEPRAFTTGLMHAAQAHGAVLRHGTVVDLLRSP
                     SGAVSGVALAGGEIVEGDAVVIAMGPWSILAARWLPLPAVLGYKGHSLVFETGGTIPA
                     EAVFLEYREASGEVLTPELFPRADGTTWVCAISTIGPLPVDPNDVAADEGAHARLEAL
                     CRNISPALAAAPIKARQACFRPVTEDGLPLIGAVPGIAGAYVATG"
BASE COUNT          463 a          820 c          846 g          480 t
ORIGIN      
        1 ggttacacca atcctgctct tataagcgtg tcagctcggt ggggcgcttc tatgacggac
       61 taagggccgc ccggtggcgt gccgagcggt tcttggggct acttgccgcg cgcgggagcg
      121 gctgcgccct gaagaccagc cgcgtgagtt gtttgctgat caccctcagc tcgtcttccg
      181 cgccgactcc ctagtccttc cagaaatcgc ggaaccgctc gggagaaagt tcgctatatc
      241 gaaccgtatg ctggatgttc ttgcggccga gatagtgttg cagcgaccga gtatccacac
      301 cctgactggc cagcttgaac ccagttgaat gccgcacatg tggggatgga ttccgaacgg
      361 catcttggcg gcctgcccaa gccgcgtccg catccggcga aaggcaaccg ccgacatcgg
      421 agcgccgcgc tcgctcatga agacatatcg tccgggttcc tgttctcgtt ggagacggcg
      481 cagggcgcga agttcggtgc cggtcaggga gtggacggaa ggcattccgt tcttaagccg
      541 atggacgtgc aaccggccat ggacgagatc aacttgctcc cagcgcaacg agcacagttc
      601 gaacactcag ataagtcgat ccgctatgat gccaagcatg atcaggaccg cactcgagta
      661 ggcgaccagt tccacgtctt gagcgcttcc ttcgatccac cgagcgatgc ccacagtcaa
      721 tacaacagcg gtcgtcgtga acatgggcgc ctcctcatcc ggtcgtacgc agccaccgcc
      781 accatgacat cggtgccgca ggtgccggta gcaggcgcgt ctgcgcttgt tcacgccaag
      841 catcgcggtc acgtcggatg tcctcaagca tcgctttcaa ctcgctcaac cgctcctcgg
      901 ccagggcgag acgggtgcgc aactcgtcca cggtctcgac aggagcaggg aacgggtaga
      961 cacgcaccga ctcggcgccg cccatgttat gaccgccctc ttccttactg cccgtgaaga
     1021 accgaccaga gattgcgcga gcgagtatcg tcttgccgcg tcccgtcagc cgcgctgttt
     1081 gccccagtga gagtgtcgtc atgtgacccc cagtatcctt ggagtgttca aaggggtcac
     1141 gcgatgcgcc acgagaccca tcccgtgacg gtgcgcacga gatggttaac gattggtgaa
     1201 gggggcatgc tgtcgccacc gccgagcact gctcatgtgg tcgaacgcgc cagcgcgcag
     1261 atcgatgggc cagccatcct gcaccaattg cagtcgaata gacgcgcgtg cgggacattg
     1321 tggtcagggc tgtactgcgc gaagatcagt gaggctcgac cctttgaaac aggtgcctag
     1381 ctatgacgac tgcagtgcgc ctggttgaac cagaccgaag tgacggtgaa agggcgccat
     1441 ttcctccagg aggatagccc ccacgagatt ggccacgccg tggccgactt cgtgcgccgg
     1501 gtccgttcgc gaaatcgtcc gtgaaggacc tgattgaaac gctcgtaaca gaccgatgtg
     1561 ctgcgcggga ccaacgatca cccagcctcg ccgctcgatg agcgctgcct tggaactgaa
     1621 aagccagcct gacacagtag cgctatgagc tcgggcaggc gcatcgtgat ctgcggtggt
     1681 ggcgcgatcg gcgccgcgat cgcctacttc acaagccgag gcggcgcccg gtcgaccgtc
     1741 atagagcgtc acatcgtcgc cggtgcggcc tccggcaagt cgggcggctt cctggccctc
     1801 gactggtgcc acggcagccc gctcgatcga ttggcacggc ggagcttcga gctccatgcc
     1861 gaactttcta ccgagctcgg cgatccttgg ggttatcggc gtctgacgac ctacggcggt
     1921 tatgccgccg aggacgatac cgcgcgcggt gcaggcgggc ggccgtggct ggctgacggc
     1981 gtggcgatca cgagtcggct cggctcaccg gagacgacag cgctcgtcga gccccgcgcg
     2041 ttcacgacag gtctgatgca cgccgctcaa gcgcatggcg cggtgctgcg gcatggtacg
     2101 gtcgtcgact tgctacgcag tccgagcgga gcagtgagcg gcgtagcact cgcgggcggc
     2161 gagatcgtcg aaggcgacgc cgtcgtgatc gcgatgggac cgtggtcgat cctggctgca
     2221 cgctggctgc cgctgccggc ggtgctcggc tacaagggac atagcttggt gtttgaaacg
     2281 ggtgggacca ttccggccga ggctgtgttc ctcgagtatc gcgaggcgag cggcgaagtc
     2341 ctgacccccg agttatttcc gcgcgccgat ggcaccacgt gggtgtgtgc gatctcgacc
     2401 atcgggccgc tgccggtcga tcctaacgat gttgcagctg atgagggtgc gcacgcgcgg
     2461 ctcgaagcgc tgtgtcggaa tatctcgcct gcgctcgccg cggcgccgat caaagcgcgg
     2521 caggcgtgct tccggccggt caccgaagac ggccttcccc ttatcggcgc cgtgccgggt
     2581 attgccggtg cctacgtggc gaccggcca
//
LOCUS       DATLJT010000277         1692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4380074,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000277 DATLJT010000000
VERSION     DATLJT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4380074"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VM910_06255"
     CDS             <1..558
                     /locus_tag="VM910_06255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI62179.1"
                     /translation="RVGPGRINGGRAMLQRYANLFAQIEQRFGVPRQILVAIWGLETD
                     FGKGDMGRLPVIRTLATLAHDCRRSELFQGELLAALKIVQRGDLPLQDMIGAYAGEIG
                     QTQFLPSSYIKYGVDFDGDGRVDLRHNVADVLASTANLLHTNGFKMGAPYGEGSANFE
                     AMREWNHAVIYRKTIAYFADRLIGR"
     gene            complement(609..1445)
                     /locus_tag="VM910_06260"
     CDS             complement(609..1445)
                     /locus_tag="VM910_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62180.1"
                     /translation="MTAIPGAAAAVTGAASGIGRALAIELAARGCNLALADRDEAGLA
                     ATAAEIGGKVEISVHRVDIGVPADIAAFAQAAIAAHPALNILVNNAGVALLGQFHEID
                     QAQMEWLFNINFWGAVHATRAFLPHLASKREAHIVNLSSIFGIVAPPGQTAYAAAKFA
                     LRGFSESLRHELQMAASPVRLSVVHPGGIATNIVRNSRPGSGVTDNTRRAQSIERFDQ
                     VAKTTPKDAALRIIEGIEKNRPRILIGSDARMMDILQRLRPATYWVPLARRLEKMAKA
                     NK"
     gene            1552..>1692
                     /locus_tag="VM910_06265"
     CDS             1552..>1692
                     /locus_tag="VM910_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVI62181.1"
                     /translation="MSLSFIVTSLIVIVSPGTGVLYTLAVALTKGARASVAAAFGCTL
                     GIL"
BASE COUNT          259 a          534 c          580 g          319 t
ORIGIN      
        1 cgcgtcggcc ccgggcgcat caatggcggt cgcgccatgc tgcagcgcta tgccaacctg
       61 ttcgcgcaga tcgaacagcg ctttggcgtg ccgcgccaga ttctggtggc gatctggggg
      121 ctcgagaccg atttcggcaa gggcgacatg gggcggctgc ctgtgatccg cacgctcgca
      181 acactcgcgc atgattgccg gcggagcgag ttgttccagg gcgaactttt ggcggcgctg
      241 aagatcgtgc agcgtggcga cctgcccttg caggacatga tcggcgccta tgccggcgag
      301 atcggccaga cgcaattcct gccgtcgtcc tacatcaaat atggcgtcga tttcgacggc
      361 gacggccgcg tcgatttgcg ccacaacgtc gccgacgtgc tcgcctcgac cgccaatctc
      421 ctgcacacca acggcttcaa gatgggtgcg ccttatggcg agggcagcgc caatttcgag
      481 gcaatgcgcg agtggaatca tgcggtgatc taccgcaaga ccatcgccta tttcgcggat
      541 cggctgatcg ggcggtaacg cgctctcacc ctccccctca agggagggtg gttcatcgcg
      601 tcacgctctt acttgttcgc cttcgccatc ttttcgagcc ggcgcgccag cggcacccaa
      661 taggtcgcgg gccgcagccg ctgcaggatg tccatcatgc gcgcgtcgct gccgatcaga
      721 attcgcgggc ggttcttttc gatgccctcg atgatcctga gtgctgcatc cttcggcgtg
      781 gtcttcgcga cctggtcgaa gcgctcgatc gattgggcgc ggcgcgtatt gtcggtgacg
      841 ccggagccgg ggcgcgaatt gcgcacgatg ttggtcgcga tgccgcccgg atgcaccacg
      901 gagagccgca ccgggcttgc cgccatctgc agctcgtggc gcaggctttc ggaaaagccg
      961 cgcagcgcga atttcgcggc cgcataagcg gtctggccgg gcggtgcgac gatgccgaag
     1021 atcgaggaga ggttgacgat atgggcttcg cgcttcgagg cgaggtgcgg caggaaggcg
     1081 cgggtggcgt gcaccgcgcc ccagaaattg atgttgaaca gccactccat ctgcgcctga
     1141 tcgatctcgt gaaactggcc gagcagcgcg acgccggcat tgttgacgag aatgttcagc
     1201 gcgggatggg ccgcaatcgc ggcctgggcg aaggctgcga tgtccgcggg cacgccgatg
     1261 tcgacgcgat gcacgctgat ctctaccttt ccgccgatct cggcggcggt cgcggcaaga
     1321 ccggcttcgt cgcgatcggc cagcgcaaga ttgcagccgc gcgcggcaag ctcgatcgcg
     1381 agcgcgcggc cgatgccgct tgcggcaccc gtcaccgccg cggccgctcc agggatcgcg
     1441 gtcattgtct gtcactcccg tcctgctggc gaaggcttat acgggcggcg catgcggggc
     1501 aaccgcgggc gtcgcttccg tggtcgggag gtgcgtggta tggggagcgc gatgtctctg
     1561 tcgttcattg tgacctcgct gatcgtgatc gtctcgcccg ggaccggcgt gctctacacg
     1621 ctggcggtcg ccttgacgaa gggggcacgc gcaagcgtcg cggccgcgtt cggctgcacg
     1681 ctcggcattt tg
//
LOCUS       DATLJT010000278         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_14974,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000278 DATLJT010000000
VERSION     DATLJT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14974"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VM910_06270"
     CDS             complement(<1..741)
                     /locus_tag="VM910_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62182.1"
                     /translation="MHRPWLKLVSLCLLALAANLAATAQAEDYPARPVKIISDSAPGS
                     AVDVTLRMVADRLGQIWGQQVLPVNQPGAGGTISARVAAEAAPDGYTLYMPALSVFLP
                     APGKAANTAYELPRDFAPIGSVTEQPMFIAAAPSLGVASLPELIALAKQRPGEISYAV
                     TGIGRLTHLTGELLQLRAGIELLLVPYSGGPSHALNDIMGGRIPLIIEGYSGLAGAIQ
                     GGNLKPLAVASARRLPDFPNLATVAETLP"
     gene            complement(1090..1764)
                     /locus_tag="VM910_06275"
     CDS             complement(1090..1764)
                     /locus_tag="VM910_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HVI62183.1"
                     /translation="MSRTRPFSRQAWDNNASIYEVIRTMPFNAQLASGTLNEARFKHY
                     ITQDAHYLIGFGRALTLAAAKAPDPDRIVQFAKAAEGALVVERALHGSFFKQYGITPR
                     AFARTPLSPACHHYVSYLLATAYGEPYEVLLGALLPCFWIYAEVGRDIHARARAANPY
                     QAWIDTYAGEEFHAAVRAVIAATDEAAAEVSPSLRARMHAAFKRATQLEWMFWDSAFR
                     LETWPL"
     gene            complement(1852..2829)
                     /locus_tag="VM910_06280"
     CDS             complement(1852..2829)
                     /locus_tag="VM910_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HVI62184.1"
                     /translation="MFDILDRIMNLDIGQRGVEKLYAPARARSTEALCAAAARHLTRT
                     AAGDCIVLITGSLTRPWVSRSIGETDGPVGVAALARALSYGFNAIPVVITDTSLIEPI
                     SATLRAAGQAIVTLDDAKRATSNKRFCSVAVVLGFPLEDGEARKEAKRIISEFRPTAM
                     IAVERAGMTPKGTYHNMLGQDYSEGRARIDYLVEEAMSSQIPTIGIGDGGNEIGMGAV
                     AEAVHQHVPHGKILCAKLATDVLLPAGVSNWGCYAIQAALAVMTEKPDLLHTAAMERR
                     LVEAAANAGLVDGNTGKCEPTVDGLPVEVHVGIVELLAATAQRGIRKSH"
     gene            complement(2846..3274)
                     /locus_tag="VM910_06285"
     CDS             complement(2846..3274)
                     /locus_tag="VM910_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62185.1"
                     /translation="MPYKGGGPAAMDVVSGQVDMLFSVLPLVLPYITSGQLRAIAIAS
                     EARSALLPDIPTVKESGFSQVVGTAWNGIVAPAGTSEDIIIKLNGEVTKVLESAETKE
                     RFAVLGMEAVGGSAEHFAEFLRAESEKWSKVIKAAQVQVQ"
     gene            complement(3286..>3520)
                     /locus_tag="VM910_06290"
     CDS             complement(3286..>3520)
                     /locus_tag="VM910_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62186.1"
                     /translation="GRSLYPDLPYDPQRELIPIALANESPIVLVVNPSVAAKSLQELI
                     GLAKAKPGALTAALVSTGSVPHLLTEMLKAAAG"
BASE COUNT          670 a         1126 c         1050 g          674 t
ORIGIN      
        1 gggcagcgtt tcggcaaccg tcgccagatt ggggaagtcg ggcagccgcc gcgccgaggc
       61 gaccgcgagc ggcttgagat ttcccccttg gatggcgccc gcgaggcccg aatagccctc
      121 gatgatcaac ggtatgcggc cgcccatgat gtcgttgagc gcgtggctcg gaccaccgga
      181 ataaggaacc aacagcaatt cgatgcccgc tcgcagttgc agcagttcgc cggtcagatg
      241 ggtgagccgt ccgatgccgg tgaccgcata ggagatttcg ccgggtcgct gttttgccag
      301 cgcaatcaat tccggcaagg acgcaacgcc gagggagggc gccgccgcga tgaacatcgg
      361 ctgctcggtc accgagccga tcggggcaaa atcgcgcggc agctcgtacg cggtgttggc
      421 cgccttgccc ggcgccggca ggaacaccga caacgccggc atgtaaagcg tgtagccatc
      481 gggcgcggcc tcggccgcga cgcgcgcgga tatggtgccg ccggcaccgg gttgattgac
      541 cggcaagacc tgttgacccc aaatctggcc cagccggtca gccaccatac gcaaagtgac
      601 gtcgacggca ctgccggggg ccgaatccga gatgatcttc actggccgcg ccgggtagtc
      661 ctcggcttgc gccgttgctg cgaggttggc tgcgagcgcc agcaggcaga gcgagacgag
      721 tttgagccag ggccgatgca ttttcattcc ccttcgagcg cctgagacgt ggccgtcgat
      781 gacggctcag tgaattggag gcgatcctgc gcagaccggt gtaacgtgag accgaaaaag
      841 ccaggtctct gaggatatgg ctgccatggg cagcgcggcc ccttccatgt cggagacttt
      901 tcggcacgtc tcgtttcgag ggcagctgtc agcacggtat atgagcaatt ctaacatgcg
      961 aatgagacga gaggacacag gttcgccgtg accgccgcag catcgtcgag gtggcgcaga
     1021 agattttggt gcagcgcggc gtaatcactc cggcgccgtc actccggcgc tttttcatca
     1081 cccccgagtc taaagcggcc aggtctccag ccggaaagcc gaatcccaaa acatccactc
     1141 gagctgggtc gcccgcttaa aggcagcatg catgcgcgcg cgcagggatg gcgacacctc
     1201 cgcggcggct tcgtcagtgg cggcgatcac ggcgcgcacc gcggcgtgga attcctcgcc
     1261 cgcgtaggtg tcgatccagg cctgatacgg gttcgccgct cgggcgcggg cgtggatgtc
     1321 gcgtcccacc tccgcgtaaa tccagaagca gggcagcagc gccccgagca agacttcgta
     1381 gggctcccca taggcggtgg cgagcagata cgaaacgtaa tggtgacacg ccggcgagag
     1441 cggtgtccgc gcgaatgccc gcggcgtgat accgtactgc ttgaagaaag atccgtgcag
     1501 cgcccgctcg acgaccagcg ccccctccgc cgctttggcg aattgcacga tgcgatccgg
     1561 atccggcgcc ttcgccgcgg caagggtcaa cgcgcgtcca aagccgatga ggtagtgcgc
     1621 gtcctgcgtg atgtaatgct tgaagcgtgc ctcgttcaac gtgcccgacg cgagctgcgc
     1681 gttgaagggc atcgtgcgga tgacctcgta gatggaggcg ttgttgtccc aggcttggcg
     1741 cgagaagggc ctcgttctgc tcatggccga tgcgattcct caaaccgaac gacgcacgcc
     1801 acgacgtgtc ggaaacagct gcaggttctg cacgcccaga catctgggaa ttcaatgaga
     1861 ttttcttatt ccacgttgtg cagtcgctgc caaaagctca acaataccca cgtgaacctc
     1921 gacgggcagc ccatcgaccg tcggctcaca cttgcctgtg ttaccgtcca ccagcccagc
     1981 gtttgcggcc gcttccacca gccgcctctc cattgcggcc gtgtggagca gatctggctt
     2041 ctcggtcatc accgccagcg cagcctgaat cgcataacac ccccagttcg atacgccggc
     2101 aggcaataaa acgtcggtcg ctaattttgc gcacagaatc ttcccgtggg gaacgtgttg
     2161 atggacagcc tccgcgacgg ctcccatgcc aatctcgttg cctccatcgc ctatgccgat
     2221 tgttggaatt tgagagctca tcgcctcctc gaccaaataa tcaatccgtg ccctaccctc
     2281 gctgtaatcc tggccaagca tgttgtgata cgtccccttt ggcgtcatgc cggcccgctc
     2341 caccgcgatc atcgccgtgg ggcgaaactc gctgattatt cgctttgcct ccttccgcgc
     2401 ctcaccatcc tccaacggga acccaaggac cacagctacg ctgcagaacc gtttgttcga
     2461 agtggcccgt ttcgcatcat cgagcgtcac gatcgcctga ccggctgccc gaagcgtcgc
     2521 gcttataggt tcaatcagcg acgtatcggt tatcaccacc ggaatcgcat tgaagccata
     2581 gctgagcgcg cgtgcgagtg ccgctactcc gacgggccca tcggtctcgc cgatgctgcg
     2641 cgacacccag ggtcgcgtta gtgagccggt tatcaggacg atgcaatctc cggccgcggt
     2701 gcgagttaga tggcgggccg cagccgcaca caaggcctcc gtgcttcggg cccgagccgg
     2761 cgcataaagc ttttccaccc cgcgttggcc tatatcgaga ttcataatgc ggtccagaat
     2821 gtcgaacatc gcgttctgcc cctcgtcatt gcacctgcac ttgcgcagcc tttatcacct
     2881 tggaccattt ctcgctttcg gccctcaaga attcggcaaa gtgctccgcg gacccgccta
     2941 ccgcctccat cccgagcacc gcaaagcgct cctttgtctc cgccgattcc agaacttttg
     3001 tcacttcgcc attgagcttg atgatgatgt cctccgaggt gccggccggg gccacgatgc
     3061 cgttccacgc agtaccgaca acttgtgaga atcccgactc tttgacggtt ggaatgtcgg
     3121 gcaaaagcgc gctccgcgcc tcgcttgcga ttgcaatcgc cctcagttgt ccggaggtta
     3181 tgtatggaag gactagaggg agcacgctga acagcatgtc gacctgaccg ctcacaacgt
     3241 ccatcgccgc aggcccgccg cctttgtacg gcacatcgag gacatttatc ccgcagcggc
     3301 cttcaacatt tcggtcagga ggtgcggtac tgaacccgtc gataccaggg ccgcggtgag
     3361 cgcgccaggt tttgctttcg ccagaccgat cagctcttgc agagatttgg ctgccaccga
     3421 gggattcacg accaacacaa tcgggctttc attcgcaagc gcgatcggga tgagctccct
     3481 ctgcggatca taaggcaaat cgggatatag actgcggcca
//
LOCUS       DATLJT010000279         1600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2312862,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000279 DATLJT010000000
VERSION     DATLJT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312862"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1121..>1600
                     /locus_tag="VM910_06295"
     CDS             1121..>1600
                     /locus_tag="VM910_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62187.1"
                     /translation="MFGMKRREFITLLCGAAAVWPLAARAQLSLPVIGFLNAESPAGY
                     QPMAAAFRQALQESGYVEGRNVAIEYRWAEGRIDRLPAMAADLVHRQVTMIAATTTPA
                     ALAAKAATTTIPIIFETGGDPVKLGLVASLNRPGGNITGVTQLSVEVAPKGLEALHEV
                     "
BASE COUNT          302 a          476 c          501 g          321 t
ORIGIN      
        1 gcggcgtctg gatcaggcgc gaatgactcc gaccggacgc agaagaggct gaaacgccgc
       61 aatgcagcag ctctcagcga gtgtttcgag gcacagggcg ctatgatcgc gcaacaaaag
      121 aaccccggag tttggcctcc gcgagcctca aaacagccgc ggttgcgtgt tttagtggca
      181 gccgcttcat cttgattttc cttccaggat tagtctttgc cccgcttcgg cggggccctt
      241 tcttttctgg aaccaatccc ccacgtcccg aattgctttg ctacggagga acgcccagaa
      301 ggcattgatc cgagccttag ccccgctcgc cggagggcgg ggcttttctc gaccccaaca
      361 tggggcgctt tggtgatgtt gtggacgtgc tagaggcttg gggggccgcg agctgatgac
      421 aatcctcata aaaccgttcc tatacctcat tgccttcggg ccgatggcgg tacttcttgc
      481 aagtgcgatc ggactagctt gctggttgct gatgatgtgt gtccgcgctc gtgcatggct
      541 ggccataatc aaaagagccc attacccgcg gcggcgaccg cgcttgcccc gaaagggcca
      601 ggcgcagcca gcctgctaat agtcgcttgg cttagccgct gcctatcgtc ggaccgctct
      661 catgtgtgct gaggtatcgt cagcaacggc tgcttaatgc gccgacgctg ctcgcgcgca
      721 ccgaagaggt gatcgaatag cgacgtattt gctgcgctgc gttcggtcgc tattggcaca
      781 aagcggacat gccagctgtg tcgctcaatg tctgctttcg ggggtaaagc agacatcgct
      841 ccctgatgac gctgtgtccg cttttgaccc aaagcggaca tagtggggac tgaaatcccg
      901 cgctgcagca gtctcctggc gaccgtcgtg tgctattgtc tgggtggtag cgcggagggg
      961 tcgcaagccc cctagattca gaacgattca ggtctgaccc aaggacttgc gaacgcctct
     1021 gcggcaggtt gagcttgcgg taaaccgaaa accgagcgtc agtcatcgct gccgattgag
     1081 gccacagcga cgacgccaga cgacagggga ggaactcccc atgttcggca tgaagcggcg
     1141 cgagttcatc acgctgctct gcggtgcggc ggcagtgtgg ccactcgcgg cgcgcgcgca
     1201 gctgtcgttg ccggtgatcg gatttctcaa cgccgagtcg cctgccggat accaaccaat
     1261 ggcggctgca ttccgtcagg cactgcaaga atccggctac gtcgaaggcc gcaacgtggc
     1321 gatcgaatat cgctgggcgg agggccgaat tgatcgactg ccggcgatgg ccgccgatct
     1381 ggttcatcgt caggtgacca tgattgccgc gaccacgact ccggcggcgc tcgcggcaaa
     1441 ggcagcgacc acgaccattc cgatcatctt cgaaacgggc ggtgacccgg tcaagctcgg
     1501 cctcgtcgcc agccttaatc ggccgggcgg caacatcaca ggcgtgaccc aattgtccgt
     1561 ggaggtagca ccgaaggggc tggaggcgct gcacgaagtg
//
LOCUS       DATLJT010000280        10976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3920817,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000280 DATLJT010000000
VERSION     DATLJT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920817"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VM910_06300"
     CDS             <1..912
                     /locus_tag="VM910_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62188.1"
                     /translation="AQQAKAATIGLLGTGSAAAQSQWTATFVQRMRELGWVEGRNLAI
                     EYRWADGRTERLSELANQLVRLKVDVIVTHNTPGPLAAKQATSTIPIVFATAGDPVGT
                     GIVASLARPGGNVTGLSSQTPDVAGKRLELLREIVPGLRQLAVLADTDNPFVKLDVSH
                     LRQAGAGLGVEVAMFEMRRGEDIDPAFEALKGRAQALYVPATPIAVVNRVRINTLALA
                     AHVPTMLGVREYVEAGGLMSYGPNFPDLFRRAADFVDKILRGTKPGDLPVEQPTKFDL
                     VINITTAKALGLEVPPTLLARADEVIE"
     gene            937..1224
                     /locus_tag="VM910_06305"
     CDS             937..1224
                     /locus_tag="VM910_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62189.1"
                     /translation="MKLPRRKFLHLAAGAAALPAVPRIAWAQAYPSRPITLIVPYPPG
                     GGADAMGRMVAQKLSPALGQQVIIENRPGAGAVIGTRAAAKSAPDGYTLSW"
     gene            complement(2087..2791)
                     /locus_tag="VM910_06310"
     CDS             complement(2087..2791)
                     /locus_tag="VM910_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVI62190.1"
                     /translation="MKENFGTKPEWLTFDCYGTLIQWDEGLIAAVERIMGRQNATVDP
                     ARFVSLFDRHEHALEQEKPHRSFRSVTAESLRLTMAELGLRHEPGDAEVLTSSISKMP
                     PFPEVVGTLDALKRRGFKLCIISNTDNDIIVGNVAQLGGHIDRVITAEQAQAYKPSRA
                     IFEHAYRELGVTKDEVVHICASPHLDHAAARAIGFRCIWIDRGTSRKPLADYTPDRTF
                     ATLDRVPAFFGTLGWG"
     gene            complement(2818..4377)
                     /gene="ilvD"
                     /locus_tag="VM910_06315"
     CDS             complement(2818..4377)
                     /gene="ilvD"
                     /locus_tag="VM910_06315"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVI62191.1"
                     /translation="MIAVVNTYQDFSPENVHLRQVAEAVSAGVRMAGGTPCMFNTFHV
                     TDSETFASVGMRYVLPSRDVVADMIELMVEGHRFDAMVLIGSGDKVMPGMVMAAARLD
                     LPAIMIYGGPTAAGVYRGRRVFLETVYDAVGEHIRGEVSAEELKGLEDNHFPFSGACD
                     TATSGNTAGIYTEALGLALPGSGTLPAGSNYQLRAAKYTGMRIMDLLAQELRPSKILT
                     RRAFENAMRVGMAVGGSTNMVLHMIAMAKEAGVDVSFDTWDALSRTTPTLVKLAPSGP
                     WGVTELNAAGGVPAVMKALGHRIERTALTVSGRTVGEIVDATEIRDRKVIRPEHDPAE
                     PEGSLFILKGSLAPGGAVVKASGVAKPMWRTTLSARAFEDEESAIDALRAGRVEPYSC
                     VIIRNEGTKGGPGMREMLGATSALMGAGLGDSCALVTDGRFSGATHGPAIGYVTPEAA
                     RGGVIGLVENGDRIAINLVERTLDLLVEQAELDRRRAAYVAPAPRVTRGYLKFYADHV
                     APASEGAVLPR"
     gene            complement(4617..5156)
                     /locus_tag="VM910_06320"
     CDS             complement(4617..5156)
                     /locus_tag="VM910_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF417 family protein"
                     /protein_id="HVI62192.1"
                     /translation="MNALIKLFARSGLLRDDLDYHLLRAAMVIIFLFFGYAKWWAYEA
                     ERLIPYISHGPLIFWLYPAFGIRGASRFLGLSEWTFGTLLFLGFWDKRAGVLGALGST
                     GTFIATVTIIPFMPDGWDQSAGGFPAMTGMVPFLMKDVVLLAVSLYLLKQDVARLLPR
                     SHSARRGRRGALVGTAQHY"
     gene            complement(5232..5768)
                     /locus_tag="VM910_06325"
     CDS             complement(5232..5768)
                     /locus_tag="VM910_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI62193.1"
                     /translation="MSRIPTPAIETATGATAELFAQIKKAVGKVPNAFASIGAHGPVA
                     LKAILNADAVLAAGSLSKQDQETIKLAVSAAVGCDYCEAAHSLLGKLAGLKPDELRRV
                     RAGSPTGDTKRDALVRFVRRLVLTRGTIDDEEFAAIRTAGYTEAQLVDISLALAVITF
                     TNVFNRINDTTLDFPAAA"
     gene            5910..6887
                     /locus_tag="VM910_06330"
     CDS             5910..6887
                     /locus_tag="VM910_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI62194.1"
                     /translation="MPFPSPPLDWLSRLLEMMPVRGHLDVRCFYGAPWRIEHAHAEPG
                     EVPYHAVLGGSGVLDDGTGGPPLRLVPGDILLLPHGSAHTLHDGSGARAIPGRARPSL
                     NLTISENTGTGDRLDVLCGRFILTPPHDRFVRDYLPGRLVVHAADHSAATARPGTGAQ
                     LAGLVSLMRSESALENLGGRAMLNALSTAMFALTLRLASEAKDPPAGLLALVGHPRLA
                     PAIAAMFNEPACAWSLPELARLCHMSRATFVRHFHNSLGRSASDLLTDIRMTLAANEL
                     RKLGMSTAAVADKVGYQSEAAFQRAFKQRMGVTPAQWRRTASAAMRDAP"
     gene            complement(7091..7249)
                     /locus_tag="VM910_06335"
     CDS             complement(7091..7249)
                     /locus_tag="VM910_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62195.1"
                     /translation="MLFDQLHRRKFITLLGGAATWPLALPSAQSRSATPSYCERPNFV
                     TALGGPQT"
     gene            complement(7327..7653)
                     /locus_tag="VM910_06340"
     CDS             complement(7327..7653)
                     /locus_tag="VM910_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62196.1"
                     /translation="MSTQLSRWALLTGLAFAAPAVGATIAEAQADAPDATNLYPGRYA
                     AVCISAPMFGCVCTTDPLREALTFTGLDSTPDNHLKEEVDSEYLRMISWLRRTCASLT
                     QPAAAR"
     gene            complement(7749..7937)
                     /locus_tag="VM910_06345"
     CDS             complement(7749..7937)
                     /locus_tag="VM910_06345"
                     /inference="COORDINATES: protein motif:HMM:NF042552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6647 family protein"
                     /protein_id="HVI62197.1"
                     /translation="MLVHEVVHHFQNVLGLKYECPQEREKLAYIAQDRWLGLFGHSLT
                     GDFALDPFSLFIKTTCFY"
     gene            complement(7924..8370)
                     /locus_tag="VM910_06350"
     CDS             complement(7924..8370)
                     /locus_tag="VM910_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62198.1"
                     /translation="MLKAFLCAIALLALAMARSVNAQEAATSPSIQLPGSECHIVLDG
                     AKFPHPANCSEQALLAAIETWLSVEFDLPIVREHPRLKLVSPAEIAALRFRGMLSNPG
                     TGIAANDHGTSSAEGDTVAVLRRDAIHLSARGVERRHAGRAIRARS"
     gene            complement(8977..9144)
                     /locus_tag="VM910_06355"
     CDS             complement(8977..9144)
                     /locus_tag="VM910_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62199.1"
                     /translation="MASQADVSILDMPAQARANRSFVRRLLDAIMESRRRKAEQFLAE
                     YDRAHKDDLSA"
     gene            9762..>10976
                     /locus_tag="VM910_06360"
     CDS             9762..>10976
                     /locus_tag="VM910_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVI62200.1"
                     /translation="MDRPDPYQERAREMAREAGLDPDGRIERPGQRSMPVWCTFRDAA
                     RKEHLAREAADAANTIVAEKPQAPQFQNSPLKVFGEHDHATVAQMRNCMSVGNVVAGV
                     VCADGHLGYAQPVGGVIAYEKQISISGVGFDIGCGNMAICLDTPFNAVEDRVGTIIRD
                     VSRTISFGVGRTNEEQVDHELFDDAEAWAASDMESYRQKAAAQLGTVGSGNHYVDLLR
                     DEDGFVWIGVHFGSRGLGHTSATRYLKAAGGKSGINVPPALIDEDSELGRRYIAAMEL
                     AGRYAYAGREWVVERVRRIVGGAVTDTVHNHHNYAWRETHEGRDVWVVRKGATPAFPG
                     ERGFVGGSMGDDAMIIEGVESPQARASLCSTVHGAGRLFGRKEAKRRFTREQMDRWLQ
                     DRGVTLIGADLDE"
BASE COUNT         2121 a         3370 c         3459 g         2026 t
ORIGIN      
        1 gcgcagcaag cgaaggcggc gaccatcgga ttgctcggca ccggtagcgc ggcggcgcag
       61 agccaatgga ccgccacctt cgtgcagcga atgcgcgagc tgggctgggt cgagggtcgc
      121 aatctcgcga tcgagtatcg ctgggccgac gggcgcaccg agcgtttgag cgagctcgca
      181 aaccagctcg ttcggctcaa ggtcgatgtc atcgtcaccc acaacactcc gggacccctc
      241 gcagcaaagc aggcgacatc gacgatcccg atcgtgttcg cgaccgcggg cgatccggtc
      301 ggcaccggca ttgtggcgag tctcgcgcgc ccgggcggca acgtcaccgg gctgtcgagt
      361 cagacgcccg atgttgccgg caagcgcctc gaactcctgc gcgagatcgt ccctggtttg
      421 cgccaattgg cggtcctagc cgataccgat aatccctttg tgaagctgga cgtgagccac
      481 cttcggcaag caggagccgg gctcggcgtc gaggtcgcca tgttcgaaat gcggcgcggt
      541 gaggacatcg atcctgcttt cgaggcgctc aagggccgcg cgcaggcgct ctacgtcccg
      601 gccactccga tcgcagtggt caatcgggtt cgcatcaata ccctggcgct cgccgcgcac
      661 gtgccgacga tgctcggggt gcgcgagtac gtcgaagcgg gaggtctgat gtcctacgga
      721 ccaaacttcc cggacctgtt ccggcgcgcc gctgactttg tggacaagat tctgcggggg
      781 acgaaacctg gcgacctccc ggtcgagcag ccgaccaaat tcgatctcgt cataaacatt
      841 acgaccgcaa aggcgctcgg cctcgaagtg ccgccgacgc ttcttgcgcg cgccgacgag
      901 gtgattgaat gatgtgaagg gagaggtaga gttaaaatga aacttccccg ccgcaaattc
      961 ttgcatctgg ccgccggcgc cgccgcgctg ccggccgtcc cgcggatcgc ctgggcgcaa
     1021 gcctatccgt cgcgtccgat tacgttgatc gtgccatacc cgcccggcgg tggtgcggac
     1081 gcgatgggcc gaatggttgc gcagaaactc tcgcctgccc ttggccaaca ggtcatcatc
     1141 gagaaccgcc ctggcgcagg cgccgttatc ggtacgcgcg ccgccgccaa gtccgcgccc
     1201 gacggctaca cgctgtcctg gtgagcggcg acccgtcacc gttccgctaa acgcctcttg
     1261 aaagtatcgt agctctcgaa ccttgacggt ttcaggcttc cgtcatcgtg ataaccaacc
     1321 agagtccagt gaccgtcaaa gttctcagcc caatatgaaa gttcccattt tccctgtcgc
     1381 ttgaaatcaa agaaaatgac atcgggcttc ccatcgccag tgctgcaatg caagccgaca
     1441 cagcggatgt tctagcggct gcccattagg cggggacgga ttatcctccc gcaaattttc
     1501 aaattctaag gtatggctct caggaggctc atatcaaagt ccagtagtct tgtgggtaac
     1561 gcgagaaggc gacggaatga ttgggtggac aacctcgcca agccaacagc agcgtgagac
     1621 aaataaggct cagtggcgaa aatatatgcg gaggtaccag atcggagtgg gctagttgcg
     1681 ttcgggtttt tgctacagtc gacgatctcc tctcaaccgc caacaaaaca gccaacacgc
     1741 caccgccgcc tcgtctcgtg ctgatgcaag ccatccgatc tgaagcaagc gcgacctcgg
     1801 aaagaaggcg acgatagctt gacgcgtcgc ccccggagcc gcggccatga tcgcgtccca
     1861 cgcgtggttg tcggcgccgg agccgcgcag aattcaaccg ctgcaccacg ctgttgcatc
     1921 cccgagactc aaaagctaac gcctgcaagc cacgttctct gaatgcaagt ttggataaac
     1981 gccggtttgg gtacaagtgc gcgacgtcgg ggagcgagcg aacttaggag gcctgaaact
     2041 cgagatctgg gtcagggttc tatgagcact tcagctcaat agtcggtcag ccccaaccaa
     2101 gcgtgccgaa gaatgccggc acgcgatcga gcgtcgcgaa ggtccggtcg ggcgtgtagt
     2161 cggccaaagg tttgcggctt gtgccgcggt cgatccagat acagcggaaa ccgatcgcgc
     2221 gcgctgccgc atggtcgaga tgcgggctcg cgcagatatg cacgacttcg tccttggtga
     2281 cgcccagctc gcggtaggca tgctcgaaga tggcacgcga gggcttgtag gcttgcgcct
     2341 gctcggccgt gatcacccgg tcgatatgac cgccgagctg cgccacgtta ccgacgatga
     2401 tgtcgttgtc ggtattggag atgatgcaga gcttgaaacc ccgacgcttc agcgcatcga
     2461 gggtgcccac cacctcggga aagggcggca tcttcgagat gctcgaggtc aggacctcgg
     2521 catcgcccgg ctcgtggcgc aggcctagct cagccatggt gaggcgcaag ctctcggcgg
     2581 tgaccgagcg gaatgaacga tggggtttct cctgttcgag cgcatgttca tgccggtcaa
     2641 acaatgagac gaaccgcgcg ggatcgaccg tcgcgttctg cctgcccatg atccgttcga
     2701 ccgccgcaat cagcccctcg tcccactgaa taagcgttcc gtagcagtcg aatgtcagcc
     2761 attccggctt ggtgccaaaa ttctccttca tggatgctcc tcctgcggac tgggcagtca
     2821 ccgcggcagt acggcgcctt cagatgccgg cgcgacatga tccgcgtaga acttgagata
     2881 gccgcgcgtg acgcgcggcg cgggcgcgac gtaggccgca cggcgccggt cgagctccgc
     2941 ctgttcgacc aggagatcga gggtgcgctc gaccaggttg atcgcgatcc gatcgccgtt
     3001 ctcgaccaag ccgatgacac cgccgcgcgc cgcctcgggc gtcacatagc cgatggccgg
     3061 cccgtgggtc gccccggaga agcggccgtc ggtgacaagg gcgcagctgt cgcccagccc
     3121 tgcgcccatg agcgccgagg tggcgcctaa catctcgcgc atgccgggac cgcctttggt
     3181 tccttcgttg cggatgatta cgcaagaata aggctcgacc ctgccggcgc gcagcgcgtc
     3241 gatggcgctc tcctcgtcct cgaacgcgcg ggcactgagc gtcgtcctcc acatcggttt
     3301 cgcgacacct gaggccttga ccacggcgcc gccgggcgcc agcgagccct tgaggatgaa
     3361 caacgatccc tctggttcgg ccgggtcgtg ctccggccgg atcaccttcc ggtcgcggat
     3421 ttcagtcgcg tcgacgatct cgccgaccgt cctgcccgac acggtcagcg ccgtgcgttc
     3481 gatacgatgg cccagcgcct tcatgacagc cggcacaccg cccgcggcat tgagctcggt
     3541 gacgccccag ggcccggagg gagcgagttt gaccagggtg ggcgtcgtgc gcgacagcgc
     3601 gtcccaggtg tcgaacgaaa cgtcgacgcc ggcctccttc gccatggcga tcatatgcag
     3661 caccatgttg gtagagccgc cgaccgccat gccgaccctc atggcgttct cgaaggcccg
     3721 ccgcgtgagg atcttggacg ggcgcagttc ttgggccagc agatccatga tgcgcatgcc
     3781 ggtgtatttc gccgcgcgca gctgatagtt gctgccggcg ggcagagtgc cggaaccggg
     3841 gagcgcaagc cccagcgcct cggtatagat gccggcggta ttgcccgacg tcgcggtgtc
     3901 gcacgcaccg gagaagggga aatggttgtc ctcgagcccc ttgagctcct cggccgagac
     3961 ctcgccgcgg atatgctcgc cgaccgcatc gtagacggtc tcaaggaaga cgcgtcggcc
     4021 cctgtagacg ccggccgccg tcgggccgcc atagatcatg atggcgggca gatcgagacg
     4081 ggccgccgcc atcaccatgc cgggcatcac cttgtcgccg gacccgatca gcaccatggc
     4141 gtcgaagcga tgcccctcaa ccatcagttc gatcatgtcg gcgacgacgt cgcggctcgg
     4201 cagcacgtag cgcatgccga ccgaggcgaa ggtctcgctg tcggtcacgt ggaaagtgtt
     4261 gaacatgcat ggcgtgccgc cggccatgcg cacgcccgcc gaaaccgcct ccgccacctg
     4321 gcgcaggtgg acattctcgg gcgagaaatc ctgataggta ttcacgacgg cgatcatcgg
     4381 ccgctcgaat tcctcgtcga tcaggccggc acctttcgcc caggcgcgct gtagcgaacg
     4441 ctcaatgccg gtgaactgct tagcactacg tcgcttctcg tcagtcatgg cttctccttt
     4501 cgggtcgaga ggccgcattt gcaaatggtg aaggaaggtc taggcgctgg gttcagcgga
     4561 ccgtctcccg tgtcttcatc tcccgcattt tcatatgtgc gaccagttac tcgtcttcag
     4621 taatgttgtg ctgttccgac gagagcgccg cgccggccgc gtcgcgcact gtgcgagcga
     4681 gggaggagcc tcgcgacatc ctgcttgagc aggtagagcg acaccgccag caggaccacg
     4741 tctttcatca ggaacggcac catgccggtc atcgccggaa agccgccagc ggattggtcc
     4801 cagccgtcgg gcatgaacgg gatgatggtg acggtggcga tgaacgtccc ggtcgaaccg
     4861 agggcgccga gcacgcctgc ccgtttgtcc cagaagccaa ggaatagcag cgtgccgaac
     4921 gtccattccg agaggccgag gaaccggctg gcaccccgaa tgccgaaggc gggatagagc
     4981 cagaagatca acgggccatg gctgatgtac gggatcagcc gctcggcctc atacgcccac
     5041 catttcgcat acccgaagaa caggaagatg atcaccatgg cggcgcgcag taggtgatag
     5101 tcgaggtcat cccggaggag tcccgacctc gcgaagagtt tgatgagagc gttcatggtt
     5161 cgattccttg gttaagttcg acccaccgcc cgcacgaagc ggtccgagcg cgcacgggcg
     5221 cggcggacgc tttaagcggc cgccgggaag tcgagggtgg tgtcgttgat gcgattgaaa
     5281 acatttgtga aggtgatcac agccagggcc aggctgatgt ccaccaactg cgcttcggtg
     5341 tagccagcgg tccggatggc ggcgaactcc tcatcgtcga tcgtgcctcg cgtgaggaca
     5401 agccgccgca cgaagcgcac cagggcgtca cgctttgtgt cgccggtggg cgaaccggcg
     5461 cggacccggc gcagctcatc cggcttcaag ccggcgagtt tgccaagcag gctgtgcgcg
     5521 gcctcgcagt agtcgcaccc aacggcagcg ctgaccgcaa gcttgatggt ctcctggtcc
     5581 tgcttgctaa gcgaaccggc ggcgagaacc gcatcagcat tgaggatcgc cttgagcgcg
     5641 accggtccat gcgcgccgat ggacgcgaag gcgttgggga ctttgccaac ggctttcttg
     5701 atctgggcga agagctccgc ggtagcgcca gtcgcggttt cgatggcggg ggtagggatg
     5761 cgggacatga tgacactcct cctcgttccg cagtcggcca gggatccagc cgacggacat
     5821 gggtgtcacc tacgctttag gaatgatact tttaattcgc atatgtctcg atattatgct
     5881 caatcatctc atactgaggc gccaccgtca tgccatttcc ttcaccgcct ctcgactggc
     5941 tgagccgcct tctcgagatg atgccggtgc ggggtcacct cgacgttcgc tgcttctatg
     6001 gcgcgccctg gcgcatcgag cacgcgcacg ccgaacccgg agaggttccc taccacgctg
     6061 tgctgggtgg atcgggcgtg ctggacgatg gtaccggcgg cccaccactg cggctcgtcc
     6121 cgggcgacat cctgctgctg cctcacggct cggcgcatac cctccacgac ggcagcggtg
     6181 cgcgggcgat tccgggccga gcacgtccca gcctgaacct caccatcagt gagaacaccg
     6241 ggacagggga ccgactcgac gtgctgtgcg gccgattcat cctcacaccg ccgcacgatc
     6301 ggttcgtgcg cgactatctc ccgggccgct tggtggtcca cgcggccgac cacagcgcgg
     6361 ccacggcgcg gcccggcact ggagcgcaac tggcgggtct cgtctcgctc atgcggagcg
     6421 agtcggccct cgaaaacctc ggcggacgcg ccatgctgaa tgcgctgtcg acggcgatgt
     6481 tcgcattaac cctgcgcttg gccagcgaag cgaaggaccc gccggccggc ctcttggccc
     6541 tggtcggcca cccgcgtctc gcccctgcaa tcgcggcgat gttcaacgag ccggcgtgcg
     6601 cgtggtcgct accggagctc gcacgcctat gccatatgtc gcgggctaca tttgtccgtc
     6661 acttccacaa tagcttgggc cggtccgcga gcgacttgtt gacggacatc cgcatgacct
     6721 tggcggccaa tgaattgcgg aagctcggaa tgtccacagc cgcggtcgca gataaggtcg
     6781 gctatcagtc cgaggctgcc ttccagcgcg ccttcaagca acgcatgggt gtcacgccgg
     6841 cgcagtggcg tcgcacggcg tccgctgcaa tgcgcgacgc accgtgacaa caggctggac
     6901 ggacgtgccg ctttgagacg aatgaaacag atattcgcgc tttgcgaggt tttctccgag
     6961 agccagccga ttaccacctt ggaggcgaac cagacatggt tcgcgaggca tcggtgtggg
     7021 catcgcccca ggggccatca tcggagcagg aattgtgtgc aattgaaagg tcagccgacg
     7081 gttacgcatc ttacgtttgc ggccctccga gggccgtcac gaagttgggc cgctcgcagt
     7141 agctcggcgt agcggagcgc gattgcgcgg acggcagcgc gagcggccac gttgccgcgc
     7201 cgccgagcag cgtgatgaac ttgcgccgat gtagttgatc gaagagcatt cgatcccctc
     7261 cagctaggcc gctttgcagg atatcgaatt ggcggtgatc agtcagcggg tgccagggcg
     7321 tcgggttcag cgagcggccg ccggttgggt gagcgatgcg caggtcctac gcaaccacga
     7381 gatcatccgc aaatactcgc tatccacttc ctctttgaga tgattgtcgg gcgtgctgtc
     7441 gagtccagta aacgtcaaag cctccctcag cgggtctgtg gtgcacacac agccgaacat
     7501 tggtgcggaa atacagacag cggcgtaacg accgggatag aggttggtcg catcgggcgc
     7561 gtccgcctga gcctccgcga tggttgctcc aactgccggt gcagcaaaag cgagcccggt
     7621 caatagagcc caacgcgaca gttgagtgga catcgggatc gatcctccgg cgcacaacat
     7681 cttccgatct ctctctagtg ggcgtccgca atcacccgcc gacatatttg ggctcgtccc
     7741 tgcaatgctc agtagaagca cgtggtcttt ataaaaagag agaatggatc gagcgcgaag
     7801 tcgcctgtga gactgtgtcc gaacaagccc agccaacgat cctgcgcgat gtaagcgagc
     7861 ttctcacgct cttgtggaca ctcgtatttg aggccaagca cattttgaaa atgatgaacc
     7921 acctcatgaa cgagcacgga tagctcggcc ggcgtgccgc cgctccaccc ctcgggcaga
     7981 tagatggatc gcgtcgcgtc gtaacaccgc taccgtatcg ccttcagcag atgatgttcc
     8041 gtgatcgttg gccgcaatcc ccgttccagg attggagagc atccctctaa aacgcagagc
     8101 cgcaatctcc gcgggagaca caagtttcag acgggggtgc tcgcggacga tcggaagatc
     8161 gaattcgacc gaaagccaag tctcgatcgc cgcaagcaaa gcttgttccg aacaattggc
     8221 cgggtgcgga aacttagccc catctaagac gatgtggcat tcgcttcccg ggagttggat
     8281 ggatggagag gtcgctgcct cttgggcatt gaccgaccga gccatcgcga gcgcgagaag
     8341 tgcgatggcg cacaaaaatg ctttcaacat gacctgagat tccgtcggtc aaagaacgtg
     8401 ccgattatgg ccccgtgacc gattcctcga gtgtgaggcg cttcacactt gtcccactcg
     8461 cgcaggatct tttttgagcg cacactgaac aggtttggtg caaaacacct gccaaacaat
     8521 gtcgagcatg caatgtgaag ggatgttcag ctccagtacc gatcacgacg ctggatctaa
     8581 gaatgtgaga tgggagcttg agcggtcatc gtcgacctct tcttaaaaat tgagcaatca
     8641 aagcgatgga cgattgagcc ttaacggtta ctaacgtcgg cctgggacac tagatgattc
     8701 gcgaaccgcc gcggcctttg ctagttggct actcgtgcca gcggcaacat atgaggattt
     8761 cgggctggtc gttgtcgccg cgaaggccat cgccagcatt ccgaggaaga gaataagtcc
     8821 caggacggca tttgctttca accagctatc gaatttccgc cgatcttccg gtgacatcct
     8881 ctgatgctcc cgtgaagtgt tactcgccac gacgctctcc atttgtgacc gagacgatgc
     8941 gacgggtgtg atccgggtta attcgcccag gtcttgttaa gcggacaggt cgtctttgtg
     9001 tgcgcgatca tattccgcca ggaattgttc ggccttgcgt cgtcgcgatt ccatgattgc
     9061 gtccaagagc cgtcgcacga acgagcggtt cgctcgcgcc tgagcgggca tgtcgaggat
     9121 agaaacgtcg gcttgcgagg ccatgaccgc ccccttgtct atagcttccg actattaaca
     9181 ctccggcact ggtgaaaatt ttaaggcaga accgacacag gatctgtgac cgccgtcaca
     9241 ctttggtgga aataggaaga agctatagcg ccttgcaatg agatatggct tttgctgacc
     9301 gaaaagcgaa gcggctcggt tcaaatggct tgtccgcggt gcagcaggcg ccgcgccgcc
     9361 ggctacttga atacgcagtc gacggcacta gcggcagtcc gggtaaccct cataggtggg
     9421 gcaggttgtg ctgacgcttg cctggctcaa tgttttgctt gccgcggagg cgaaagcggg
     9481 actgcaggtc aaggcaatcg cggaggacag gatgaaagtg atgagacgca tgggggacct
     9541 cgctttgggt agggacaaac ggattggcga cttcagctca accgcgacat gcggtaactc
     9601 aatctgatgg gcagcggtag acctgaagcc tgccagctca ttctgtccga caagtgctga
     9661 cggcctacac gggccaaact tcttggcaat gaagctctct tcaacttcac gaggcagacg
     9721 cagggttgcg tcggcaagcc gcaatagcca agtgcaaggc tatggaccga cccgatccat
     9781 accaagagcg tgcgcgtgaa atggcccgcg aagctggcct cgaccccgac ggcaggatcg
     9841 agcgaccagg ccagcggtcg atgccggtct ggtgcacgtt ccgtgatgcc gcgcgcaaag
     9901 agcacttggc gcgggaggcc gcggacgccg cgaatacgat cgtagccgaa aagccgcagg
     9961 cccctcaatt ccagaacagc ccgctcaagg tattcggcga gcacgaccac gcgacggttg
    10021 cgcagatgcg caattgcatg tctgtcggca acgtcgtcgc cggcgtcgtc tgcgccgatg
    10081 gccatctcgg ttacgcacaa ccggtcggtg gcgtcattgc ctacgagaag cagatcagca
    10141 tttccggcgt tggcttcgac atcggctgcg ggaacatggc catctgtctc gatacgccgt
    10201 ttaacgcggt cgaggaccgc gttggcacaa ttatcaggga cgtgtcgcgc acgatttcgt
    10261 ttggcgtcgg tcgcaccaac gaagagcagg tcgaccacga gctcttcgac gatgccgagg
    10321 cctgggctgc atctgacatg gaatcatatc gccagaaggc ggcggcgcag ctcggcaccg
    10381 tcgggtccgg gaatcactac gtggatctcc tgcgcgatga ggatggcttc gtctggatcg
    10441 gcgttcattt cggtagccgc gggctcgggc acacgagcgc gacccggtat ctcaaagcgg
    10501 ccggcggcaa aagcggcatc aacgttccgc ccgccttgat tgacgaggac agcgaattag
    10561 ggcgccggta catcgcagcg atggagctcg cgggacgtta tgcctacgcg ggccgcgaat
    10621 gggtggtgga gcgggtgcgg cgaatcgtcg gcggcgccgt gaccgacacg gtgcacaacc
    10681 accataacta cgcctggcgt gagacccacg aggggcgcga tgtatgggta gtgcgcaagg
    10741 gcgcgacgcc ggcgttcccc ggtgagcgtg gctttgtcgg cggttcgatg ggcgacgacg
    10801 ccatgatcat cgaaggcgtc gagagcccgc aggcacgcgc ctcgctctgc tcgaccgtcc
    10861 acggcgccgg ccggctgttc gggcgcaagg aggccaagcg acgtttcacc cgcgagcaaa
    10921 tggaccggtg gctgcaagac cgcggtgtga cgctgatcgg tgccgacctg gacgaa
//
LOCUS       DATLJT010000281         2185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_934608,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000281 DATLJT010000000
VERSION     DATLJT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934608"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..717)
                     /gene="nusG"
                     /locus_tag="VM910_06365"
     CDS             complement(163..717)
                     /gene="nusG"
                     /locus_tag="VM910_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HVI62201.1"
                     /translation="MATAATLTADKRWYIVHAYSNFEKKVAESIREQAKQRGLDDLFE
                     QVLVPTEKVTEVRRGRKIDAERKFFPGYVLVKMKLTDEAFHLIKNTPKVTGFLGAENK
                     PMPISETEAMRILHQVQEGVERPKASVSFEIGENVRVADGPFASFSGVVEEIDEARSR
                     VKVAVSIFGRATPVELEFGQVEKV"
     gene            complement(717..908)
                     /gene="secE"
                     /locus_tag="VM910_06370"
     CDS             complement(717..908)
                     /gene="secE"
                     /locus_tag="VM910_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVI62202.1"
                     /translation="MAISPFKFLQEVRSETAKVTWPTRRETTITTIMVFVMVALASIF
                     FFAADQIIRFLITMILGIH"
     gene            complement(1173..1248)
                     /locus_tag="VM910_06375"
     tRNA            complement(1173..1248)
                     /locus_tag="VM910_06375"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1213..1215),aa:Trp,seq:cca)
     gene            1409..>2185
                     /locus_tag="VM910_06380"
     CDS             1409..>2185
                     /locus_tag="VM910_06380"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI62203.1"
                     /translation="MNAQAAAKPTTSPQTPPLPQVRPDQIGLSPARLQRMSDAFKREI
                     DKGTVPGVTVMVARRGQIGWFEAIGRQNPATSQPMSHNTIFRIFSMTKPIVSLGIMML
                     LEDGHFLLNEPVAKFIPEFAKQKVGIEHNGRLELVALQRPMTIQDLLRHTSGITYDHT
                     GNSLVQQLYQQSRLRSRKITNAEHAAMVAELPLICQPGAEWNYSRSTDILGRIIEIVS
                     GKTLGAFLTERILAPLQMAETAFFTGEENAGRLAEPFPTDP"
BASE COUNT          439 a          711 c          626 g          409 t
ORIGIN      
        1 tttgccgctc ccgccggcac ctgcaatttc aggtatccgg tcactttctt tgccatgtct
       61 tgctcccgtt gtgccggttt gcgccggcgg tttcaggttt cgtggttcgg atcgcgcggc
      121 tggcagaccg cccgcttcct cccacgtcct cgctcagctc ggtcagacct tctcgacctg
      181 accgaattcc agttcgaccg gcgtcgcgcg gccgaagatc gaaacggcga ccttcacgcg
      241 cgagcgcgcc tcgtcgattt cctcgaccac accggagaac gacgcgaacg gaccgtccgc
      301 cacgcgtacg ttctcgccga tctcgaacga caccgacgcc ttcggccgct caacgccttc
      361 ctgcacctgg tgcaggatgc gcatggcttc ggtctccgag atcggcatcg gcttgttttc
      421 ggcgcccagg aaacccgtta ccttcggcgt gttcttgatg agatgaaagg cctcgtcggt
      481 cagcttcatc ttcaccagca catagcccgg gaagaattta cgctcggcgt cgatcttgcg
      541 cccgcggcgc acttcggtga ccttttcggt cggcaccagc acctgctcga acaaatcgtc
      601 aagcccgcgc tgcttcgcct gctcgcggat cgattcggcg accttcttct caaaattgga
      661 ataggcgtgg acaatatacc agcgcttgtc cgcggtcaga gttgcagcgg ttgccatcag
      721 tgaatgccca gtatcatggt gatcaaaaag cggatgatct gatccgcggc gaaaaagaaa
      781 atcgacgcca acgcaaccat tacgaacacc atgatggtgg tgatcgtggt ctcgcgacgg
      841 gtcggccaag tgaccttggc ggtctccgag cgcacttcct gcagaaattt gaacgggctg
      901 atagccatcg aggttccgcg tccgttgccg gacgaattga ctgtgaagat taaggaatcc
      961 gaacaggccg aagccgccca accctcgtca gaaggatgag ccaaaaagag aggctcgctt
     1021 gttcggggat ttcaaagggc cgcgccggaa atccgtcgaa gcgggccggc tgcaggtggc
     1081 gggtatgtac tgccaagcct gccgaacgtc aagttgccct gaccaaaggc agcggcggac
     1141 cgctggggga tcaagggctt ggcggccgat cttggcagga gtggcagggc tcgaacctgc
     1201 gacccccggt tttggagacc ggtgctctac caattgagct acactcctac gagaccgcag
     1261 cgggcgcgaa gcgcggctct gcaatccgcg ccgtttcaag catagggcgc tgcctgtttg
     1321 caagggtgaa gccggcaaac gccctgttca agccaccggt tctgcgccag actccgctta
     1381 agccgacaag agccaacggg agaagccgat gaacgcgcaa gctgccgcaa aaccaacaac
     1441 ctcgccgcaa acgccgcccc ttccccaggt cagaccggac cagatcgggc tttcgcccgc
     1501 ccgtctgcaa cgcatgtcgg acgccttcaa gcgcgagatc gacaagggaa ccgtaccggg
     1561 cgtgaccgtg atggtggcgc gacgcggcca gatcggctgg ttcgaggcta ttggccgaca
     1621 aaacccggcc acaagtcagc caatgtccca caacaccatc ttccgcatct tctcgatgac
     1681 caagccgatc gtttctctcg gcatcatgat gctgctcgag gacgggcatt tcctgctgaa
     1741 cgagcccgtc gccaaattca ttccggaatt cgcaaagcag aaggtcggca tcgagcataa
     1801 cggccggctc gaactcgtcg cgttgcagcg gccgatgacg atccaggatc tgttgcggca
     1861 cacgtccggc atcacttacg accataccgg caacagtctg gtgcagcagc tctatcagca
     1921 gtctcgcctg cgcagccgca agatcaccaa tgccgagcat gcggcgatgg tcgccgagct
     1981 gcccttgatc tgccaacccg gcgcggaatg gaactacagc cgctccaccg acatcctggg
     2041 gcgcatcatt gagatcgtca gcggcaagac gctcggcgcg tttctgaccg agcgcattct
     2101 cgcaccgctg cagatggccg agaccgcctt cttcaccggc gaagaaaatg ccggccgcct
     2161 cgccgaaccc ttcccgaccg atccc
//
LOCUS       DATLJT010000282         1578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2313188,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000282 DATLJT010000000
VERSION     DATLJT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313188"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VM910_06385"
     CDS             complement(<1..762)
                     /locus_tag="VM910_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI62204.1"
                     /translation="MTVLRKMRTDEAVLRKARMLIGGRWVDSASGKVLEVEDPAHRHP
                     IAEVPRGDAEDVARAVPAAADAFPAWSRTIPRERGRLLARIADALETRVEELARTIAL
                     ETGNALRTQARPEARTTVDIIRYFGGLGGELKGETIPFGEHVLSYTRREPLGVVGAII
                     PWNAPVLLAALKIGPALCAGNTLVLKSAEDAPLGVLMLAEICQEFLPPGVLNVLTGLG
                     EECGAPLANHPMVSKLSFTGSTEVGKLIMRAASDRI"
     gene            complement(807..1316)
                     /locus_tag="VM910_06390"
     CDS             complement(807..1316)
                     /locus_tag="VM910_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62205.1"
                     /translation="MATKTRAKTKRKKTTVSKAARRRKTVRKTRVIRKKAAKKAAPRR
                     APRTKAKSTAKVAVKPTPVSQRAQPEAVKPPPSRPAQPSAAEQRIGAVTHYYSHLLVV
                     AMQLEPGTTLRVGDVIHIRGHTTDFTQKVESLEVDHAPVTEVRPNDDFGLRVVEHARE
                     HDIVYKVRS"
BASE COUNT          252 a          500 c          493 g          333 t
ORIGIN      
        1 gatgcggtct gaggcagcgc gcatgatgag cttgccgacc tccgtcgagc cggtgaagga
       61 gagcttgctt accatcgggt ggttggcgag cggagcgcca cattcctcgc cgagcccagt
      121 caacacgttg agcacgccag gcggcaagaa ttcctgacat atttcagcga gcatcaggac
      181 gccgagcggt gcatcctctg cggacttcaa gacgagcgtg ttgccggcgc aaagcgcggg
      241 gccgatcttc agagcagcca gtaggactgg cgcgttccac gggatgatgg cgccgacaac
      301 gccaagcggc tcacgccgcg tgtagctcag aacatgctcg ccgaagggga tcgtttcgcc
      361 cttgagttcg ccgcccaagc cgccgaagta gcggatgatg tcgactgtgg tccttgcctc
      421 aggccgcgcc tgggtgcgaa gcgcgttccc ggtctcgagc gcaatcgtgc gcgccagctc
      481 ctcgacccgt gtctcgagcg catcggcgat ccgtgcgagc aagcgcccac gctcgcgtgg
      541 gatcgtgcgc gaccaggcgg gaaaggcatc ggccgcggct ggcacggcgc gcgcgacgtc
      601 ctcagcgtcc ccccggggaa cctcagcgat cgggtgccgg tgcgccgggt cctcgacctc
      661 aagcaccttg cccgaagcgc tatcaaccca cctcccgccg atcaacatgc gcgctttcct
      721 aagcaccgcc tcgtcagtgc gcattttcct aagaaccgtc atgaccgcct ccgtcgttcc
      781 tttggagctt ggttcgtcca gcggcgctac gatcgcactt tgtacacgat gtcgtgctcg
      841 cgcgcatgct cgacgaccct cagtccgaaa tcgtcgttcg gccggacctc ggtcactggc
      901 gcatgatcaa cctcgaggga ctcgaccttc tgggtgaagt cggtggtgtg tccgcggatg
      961 tggattacat cgccaacgcg cagcgtagta ccgggttcga gctgcatggc cacgactaag
     1021 aggtggctgt aatagtgggt cacggcgccg atgcgctgct ccgccgcact aggctgtgca
     1081 gggcggctcg gcggcggttt gactgcttca ggctgcgccc gttggctgac tggcgtcggc
     1141 ttgaccgcga cctttgcggt cgacttggct ttggtgcgtg gcgctcgccg gggcgccgcc
     1201 ttcttcgctg ctttctttct gattacccta gtttttctaa ccgtctttct gcgccttgcc
     1261 gccttgctga cggtcgtttt cttgcgtttc gtcttggctc tcgtttttgt cgccatggca
     1321 gcctccctga cgctcactgc atttggaaag tctttgtccg atcgttgccg aataagaagg
     1381 taagtcagca ggccagacgg ctgtcatcga ttatagacca tcgacaggcg agtacagcag
     1441 ctgactgtgg cagcgtgaat agatccaggc gccggtcacg gcaccgtgtt cagtcagctg
     1501 atcgatattg ccacgggcac cgagcgtgcc agtctccgcc ggtatcgatc gcgcctcgcc
     1561 gtattggttg ggctcact
//
LOCUS       DATLJT010000283         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1164666,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000283 DATLJT010000000
VERSION     DATLJT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164666"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VM910_06395"
     CDS             <1..258
                     /locus_tag="VM910_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI62206.1"
                     /translation="PEMLRIGYLTAMVPLEALDEEVDRLATVLAGNAPIAMRGMKRTI
                     NEIARGRLDEAAADRRAVESMHSTEIQEGIRAFAEKRPPKF"
     gene            684..851
                     /locus_tag="VM910_06400"
     CDS             684..851
                     /locus_tag="VM910_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62207.1"
                     /translation="MTNPSLDEIIARLQSCIVDARALQLKMLERILSIALLEAYENIE
                     KTRRKGDNGGG"
     gene            complement(1070..1789)
                     /locus_tag="VM910_06405"
     CDS             complement(1070..1789)
                     /locus_tag="VM910_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HVI62208.1"
                     /translation="MADAASTTKASYTSEERIGVLEELLNERFSVRAFLPQLVPRETI
                     ARVLAVAQRTASWCNSQPWQVIIASGEARERFRKAIYAEAGSGAPEDTDFPFPREYKG
                     VYLERRRESGFQLYNTLGIPRGDKAAYARQMLENYNFFGAPHVAIIHTDEPLGVYGAI
                     DCGGYVSNFILAAQALGLGTIPQAALARHSGLIRRHFNLADDRRVVCGISFGYTDRGH
                     RANSYRTSRAKIADTITFVDS"
     gene            complement(1824..>2516)
                     /locus_tag="VM910_06410"
     CDS             complement(1824..>2516)
                     /locus_tag="VM910_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI62209.1"
                     /translation="DPNVPKHKGLTMFFLDMKSKGVEVRPIKQANAMQEFNEVYFTDV
                     VIPDSQRLGNVGDGWNVSLTTLMNERMSIGARLATGFPELFEFCSNLMLEDGPAIDDR
                     NVRAKLASWAVKASGLKYTSYRAISALSRGERPGPENSIGKLVAGSMLQDIATYAMDL
                     EGAAGVLTGEAEETARGQFQQMLLSSPSMRIAGGTDEVLRNIIAERVLGLPGDIRVDK
                     DVPFNKIPTKGR"
BASE COUNT          485 a          765 c          761 g          505 t
ORIGIN      
        1 ccggagatgc tgcgcatcgg ttatcttacc gcgatggtgc cgctggaggc gctcgacgaa
       61 gaggtcgacc gtttggcgac ggtccttgcc ggcaacgcgc cgatcgcaat gcgcggcatg
      121 aagcggacga tcaacgaaat cgcccgcgga cggctggatg aagcggccgc cgaccggcgc
      181 gccgtcgaaa gcatgcattc cactgaaatc caggaaggca tcagggcgtt cgcggaaaag
      241 cggccgccga aattctgaga cgcccggcgc ttgcgttcac gggcaatatg gcttgctacc
      301 gccccgcccg cacggaaaag cgcacccgaa gatacaacct gcgcattatc ttttctttgt
      361 tattgactca gggttgtaga cggctagttt gatcgccggg cgggagatca tttcaggatg
      421 ccggagtcga agccaagtgg gaaaccgagc ggcgcgtcgc cgatcaagcg gcgtcattcg
      481 cccggttcca ccggatattc ctcttctttt tccacgagtc gttcggccat tgcgatcaac
      541 tcgtcgcgat ggcgctgctc gcgcagcctg aggaagcagc gcatcagccg caagccgcac
      601 gctgtcgtct cgtcaacctt caatgctcca agctttggcg gcatctcgat gcgacccact
      661 gatgaaattg cggatttgaa gtcgtgacga atccctcgct cgacgaaatt attgcgcggc
      721 tgcaatcatg catcgttgat gccagggcgc tacaactcaa gatgctggag cggattttgt
      781 caatagcgct gcttgaagct tatgaaaaca tagagaaaac gcgaagaaag ggcgataacg
      841 gcggcggtta gagcaagcac gctgcccggc gccggcaatg caccgaacga gcgcgccgaa
      901 catcattata acaggctgat ggagcgcgat agctttatgt cgtcgcatcg cgttcgagct
      961 tattgttcgc gcatgagctc tgcccaaaca cgttccgctt ttgtcgcgcg ggaaaaccgg
     1021 taaccacccg ctatccaggt gcggggcagg ctttcttccg gttcgtacgc taactgtcca
     1081 caaacgttat ggtatccgcg attttggcgc gcgaagtacg gtagctgttc gctctgtggc
     1141 cgcggtcggt atagccgaag gaaatgccgc agaccacgcg gcgatcgtcg gcaagattga
     1201 aatgccggcg gatcaggccg gagtgacggg cgagcgccgc ctgcggaatg gtgccaagcc
     1261 cgagcgcctg ggccgccaat atgaaattgg agacataacc accgcaatcg atcgcgccgt
     1321 aaaccccaag cggctcgtcg gtatggatga tcgcgacatg cggcgcgccg aaaaagttgt
     1381 agttctccag catctgcctg gcgtaggcag ccttgtcgcc tctgggaatg ccgagtgtgt
     1441 tgtagagctg aaagccgctt tcgcgccggc gctcgagata gacgccctta tattcgcgcg
     1501 gaaagggaaa gtcggtgtcc tcgggcgcgc cggagccggc ttccgcgtag attgccttgc
     1561 ggaagcgctc cctggcctcg ccgcttgcga tgatcacctg ccacggctga ctgttgcacc
     1621 acgaggcggt gcgctgcgcg acggcgagaa cccgcgcgat ggtttcccgc ggcacgagtt
     1681 ggggcagaaa ggcccgcacc gagaagcgct cattgaggag ttcctcgagc acgccgatgc
     1741 gctcttcgct cgtatagctc gctttcgtcg tgctcgccgc atccgccatg gcaaacgatc
     1801 ctctttcttt cttaccttcc ccgtcagcgt cctttggtcg ggatcttgtt gaagggaacg
     1861 tccttgtcga cgcggatatc gccgggcaaa cccagcacgc gctcggcaat gatgttgcgc
     1921 aagacctcgt ccgtgccgcc ggcgatccgc atcgagggcg acgacagcag catctgctgg
     1981 aactgaccgc gcgccgtctc ttcggcttcg cccgtcaaca ccccggccgc gccttccaga
     2041 tccatcgcgt aggtcgcgat gtcctgcagc atcgagcccg cgaccagctt accgatcgag
     2101 ttttccggcc caggacgttc tccccgggag agcgccgaga tcgcccggta gctcgtgtat
     2161 ttcaggccgc tcgcctttac cgcccagctc gcgagttttg cgcgcacatt gcggtcgtcg
     2221 atcgcaggtc cgtcctccag catcaaattg gagcagaact cgaagagttc ggggaaaccc
     2281 gttgcaagcc gcgcgccgat cgacatgcgc tcgttcatca gcgtggtcag cgatacattc
     2341 cagccgtcgc cgacgttgcc gaggcgctgg ctatccggga tcacgacgtc ggtgaaatag
     2401 acctcgttga actcctgcat ggcgttggcc tgcttgatgg gccgcacctc gacacctttg
     2461 ctcttcatgt cgaggaagaa catggtgagt cccttgtgct tgggcacatt gggatc
//
LOCUS       DATLJT010000284         1677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3461936,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000284 DATLJT010000000
VERSION     DATLJT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461936"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1677)
                     /locus_tag="VM910_06415"
     CDS             complement(<1..>1677)
                     /locus_tag="VM910_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HVI62210.1"
                     /translation="VAGGRADQVALIHDSPLTNSITKFTYAELLKEVQTLAAVMRDFG
                     VEKGDRVILYMPMVPEAVVAMLACARIGAVHSVVFGGFAAKELATRIDDARPKLIFSA
                     SCGIEPGRIVQYKPLLDEAIELAEAKPQSCIILQRPQQRCELTAGRDHDWATLRRKAL
                     DAGKLAPCVAVAATDPLYILYTSGTTGIPKGVVRDNGGHLVSLKWSMFNLYGIKPGEV
                     WWCGSDIGWVVGHSYIVYGPLLQGATSIMYEGKPVGTPDAGAFWRVISEHKAVALFTA
                     PTAFRAIKKEDPDGAFIRKYDLSKFRTLFLAGERADPPTVEWAEAQLKVPVIDHWWQT
                     ETGWCIAGNPVGLGMLPVKHGSPTVPMPGYQVDVVDESAKPVPTGSMGSIVIKLPMPP
                     GCLPTLWQQEERFTESYLSEFPGYYKTSDAGYKDEDGYVFVMGRTDDIINVAGHRLST
                     GGMEEILASHPDVAECAVLGVKDAIKGEVPCGFLVLKAGVTRSPAEIEKEIVALVREK
                     LGPVAAFKLAITVGRLPKTRSGKILRGTIKKIADGDSWTMPATIEDPKVLD"
BASE COUNT          305 a          553 c          516 g          303 t
ORIGIN      
        1 atcgagcacc ttgggatcct cgatggtcgc cggcatggtc caggaatctc cgtcggcgat
       61 cttcttgatg gtgccgcgca ggatcttgcc cgaacgcgtc ttcggcaacc ggccgaccgt
      121 gatcgcaagc ttgaaggcgg ccacaggacc gagcttttcg cgcaccagcg ccacgatctc
      181 cttttcgatc tcggcgggcg aacgcgtcac gccggccttc agcaccagga agccgcaggg
      241 cacttcgccc ttgatcgcgt ccttgacgcc gagcaccgcg cattcggcga catcgggatg
      301 cgaggcgaga atttcctcca taccgccggt ggagagacgg tggccggcga cattgatgat
      361 gtcgtcagtg cggcccatca cgaagacata gccgtcctcg tccttgtagc cggcgtccga
      421 ggttttgtag tagcccggaa attcgctcag ataggattcc gtgaaacgct cctcctgctg
      481 ccagagcgtc ggcagacaac ccggcggcat cggcagcttg atgacgatcg agcccatgct
      541 gcctgttggc accggctttg cggactcgtc gaccacgtcg acctgatagc ccggcatcgg
      601 caccgtcggc gagccgtgct tcacaggcag catgcccagt cccaccggat tgccggcgat
      661 gcaccagccg gtctcggtct gccaccagtg gtcgatcacg ggcaccttga gctgcgcttc
      721 cgcccattcc actgtcggcg gatcggcgcg ctcgccggcg agaaacagcg tgcggaattt
      781 cgacagatcg tatttgcgga tgaacgcacc gtccggatct tccttcttga tggcgcggaa
      841 cgcggtcggc gcggtgaaga gggcaaccgc cttgtgctcg cttatcacgc gccagaacgc
      901 gccggcgtcg ggcgtaccga ccggcttgcc ctcgtacatg atcgaagtcg cgccctgaag
      961 cagcggtcca tagacgatat aggaatgccc gaccacccag ccgatgtcgg agccgcacca
     1021 ccagacctcg ccgggcttga tgccatagag attgaacatc gaccatttca gcgagaccag
     1081 atgcccgcca ttgtcgcgca ccacgccctt ggggattccg gtcgtgcccg aggtgtagag
     1141 aatatagagc ggatcggtcg cggcgaccgc cacgcaaggc gcaagcttgc cggcatcgag
     1201 cgctttgcgg cgaagcgtgg cccagtcatg gtcgcggcca gccgtgagct cgcaacgctg
     1261 ttgcggacgc tgcaggatga tgcagctctg cggtttcgct tcggcaagct cgatcgcctc
     1321 gtcgagcagc ggcttgtatt gcacgatacg gcccggctcg atcccgcagc ttgccgaaaa
     1381 gatcaatttc ggccgcgcat cgtcgatgcg ggtagcaagc tcctttgcgg caaagccgcc
     1441 gaacaccacg ctgtgcaccg cgccgatgcg cgcgcaggcc agcatcgcaa cgacggcttc
     1501 cggcaccatc ggcatataga ggatgacgcg gtcgcccttc tcgacgccga aatcccgcat
     1561 gaccgcggcg agcgtctgca cctccttcaa caactcggca taggtgaatt tcgtgatcga
     1621 gttggtcagc ggcgaatcat ggatcagcgc cacctgatcg gcgcggccgc ccgcgac
//
LOCUS       DATLJT010000285         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_15590,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000285 DATLJT010000000
VERSION     DATLJT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15590"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VM910_06420"
     CDS             complement(<1..905)
                     /locus_tag="VM910_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62211.1"
                     /translation="MSDTALPLPRLAGAERAPRSARARGYWRTVGRRLSRDYVTLFFA
                     LVIAVIVLAAILAPLIAPFDPNATSIVNRLKPIGYKGLFILGTDELGRDVLSRLIYGG
                     RISLLMGLLPVGLATCIGGFLGLIAGFAGGWVNALIMRIMDVFYAFPSVLLAVAISGT
                     MGGGIVVSLVALTLVFTPPLCRVSEAATTQIRSRDFVDAARASGASSLSIMLYHVLRN
                     VLAPVLIYASSLVSVSILLAAGLSFLGLGVEPPTPDWGLMLSTLRQSIYVQPVICALP
                     GAAIFVCSLSFNLVSDGLRQAMDIKA"
     gene            complement(913..1869)
                     /locus_tag="VM910_06425"
     CDS             complement(913..1869)
                     /locus_tag="VM910_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62212.1"
                     /translation="MWYYAARRIMLAIPIAIGVTIICFCLVYLAPGDPVQNLLPPDAN
                     QADVAYLKHAYGLDQPVPVQYFKWLARAVAGDFGKSIQTSRPVLNEVLQALGNTLFVS
                     TGAVMLAFPLALVLGTLAAYYVGRAPDRIATGVAVIGISVPNYWFAIVLIIIFAVELH
                     VLPAAGMGPGGSESFDPLRWSQLRYAVLPVLATSLVPLGIIMRSTRSAVADMLSQDFI
                     LTLRAKGLRGWGVLRHALRNALPQVLTVMGLQFGYLVGGSILIETIFTWPGTGFLLNK
                     AILTRDIPMLQGAMLVLALWFVLINLVVDLLQTAIDPRIKRG"
     gene            2148..3737
                     /locus_tag="VM910_06430"
     CDS             2148..3737
                     /locus_tag="VM910_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62213.1"
                     /translation="MLDLHKTFAAAALMAAGVLALQPQAASAESVLRIAMTAGDIPDW
                     TGQPDQGFEGYRFVGFALYDGLVNWDLSRSDREADIKPALATKWSADPGDLKKWIIEL
                     RHDVKFHDGCDWNADAAVWNFERLTSKASPAFSPLHFARARSRSNDIDRVEKIDAYTI
                     AIHTNAIVSLFPYNLPFLLMMSPCAMAQAGNDYQAYAKAPAGTGPYKFDKVVPHQRLE
                     LVKNSDYWDRGRVPKQDRVVLLPMPEASTRVAALLSGQIDWVEAPPPDALDRLKAAGM
                     NIVTNVYPHTWPYLLNFMRGPFTDLRVRQAANYAVNRNEMAEMLNGIAVPSYGVFVPS
                     QRYYGHPFEHKTDPAKATALLKAAGCYPCAITVAISTSGSGQMQPLPMNELVKEQLEK
                     VGFKVAFETLDWNTILTIYIQGAVKYPQYDAVNFSSGATDPLNYLKTVMSSYKAPNGA
                     NWGGYSNPKVDELALRAFATFDEAERDKLVAHIHETTSADAVRVFIVSDLNPRALSPK
                     LSGFVQARSWFQDITPVAVGN"
     gene            complement(3892..>4229)
                     /locus_tag="VM910_06435"
     CDS             complement(3892..>4229)
                     /locus_tag="VM910_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HVI62214.1"
                     /translation="FSARYDLDRLRGVFSRPDHKLAGESYVGRVLVLDAAKGGVATAW
                     MLREMKARGVVPAALVLNAANPIMVQGAAFADFTMISGFDRDITQAIANGAMVEVDPT
                     GERPFIRVI"
BASE COUNT          848 a         1384 c         1347 g          650 t
ORIGIN      
        1 gccttgatgt ccatcgcctg ccgcagcccg tcgctgacca gattgaacga cagggagcag
       61 acgaagatcg cggcgcccgg cagcgcgcag atgaccggct gcacatagat cgactgccgc
      121 aacgtcgaga gcatcaatcc ccaatcgggt gtcggcggct cgacgccgag gccgagaaag
      181 gacagcccgg ccgccagcag gatcgacacc gagaccaggc tcgaggcgta gatcagcacg
      241 ggcgcgagca cattgcgcag cacatggtag agcatgatcg agaggctcga tgcgccgctc
      301 gcccgtgcgg cgtcgacgaa gtcgcggctg cgaatctgcg tcgtcgccgc ctccgatacg
      361 cggcagagcg gcggagtgaa cacgagcgtg agggcaacga ggctgaccac gatcccgccg
      421 cccatcgttc ccgagatggc gaccgcgagt agcaccgacg ggaacgcgta gaacacgtcc
      481 atgatgcgca tgatcagggc gttgacccag cctccggcaa agccggcaat gagaccgaga
      541 aacccgccga tgcaggtggc gaggccgacc gggagcaggc ccatgagcag cgatatgcgc
      601 ccgccgtaga tcaagcgcga gagaacgtcg cggccgagct cgtcggtgcc gaggatgaat
      661 aaccccttgt agccgatcgg cttcaggcgg ttgacgatgc tggtcgcgtt cgggtcgaac
      721 ggcgcgatga gcggcgcgag aatggcggcg agcacgatga cggcgatgac cagtgcgaag
      781 aacagcgtca cgtaatcccg gctcagacgg cggccgacgg tgcgccaata gccgcgcgct
      841 ctcgcgctac gcggcgcccg ctccgctccg gcaagccgcg gcaatggcag cgcggtgtcg
      901 ctcatgcagc cctcagcccc gcttgatgcg cgggtcgatc gcggtctgca gcagatcgac
      961 cacgagattg atcagcacga accacagggc aagcaccagc atcgccccct gcagcatcgg
     1021 aatgtcgcgc gtcagaatcg ccttgttgag caggaatccc gtgcccggcc aggtgaagat
     1081 cgtctcgatc aggatcgagc cgccgacgag atagccgaac tgcagaccca tgacggtgag
     1141 tacctgcggc agcgcgttac gcagcgcgtg gcgcagcacg ccccatccgc gcaggccctt
     1201 cgcgcgcaga gtgaggatga agtcctgtga taacatgtcg gcgacggcgg atcgcgtgct
     1261 gcgcatgatg atgccgagcg gcaccagcga tgttgcgagc accggcagca ccgcgtatct
     1321 gagttggctc cagcgcaacg gatcgaagct ctccgagccc ccagggccca tgccggccgc
     1381 aggcaggaca tgcagctcga ccgcgaagat gatgatcagg acgatggcga accagtagtt
     1441 cggcacgctg atgccgatca cggcgacacc ggttgcgatg cggtcgggcg cgcgcccgac
     1501 gtaatacgcg gccagcgttc cgagcactaa cgccagcggg aatgccagca tcacggcgcc
     1561 ggtcgacacg aatagcgtgt tgccaagtgc ctgcagcacc tcgttcagca ccggacggct
     1621 ggtctggatc gacttgccga agtcgccggc gacagcgcgc gcgagccatt tgaagtattg
     1681 caccggcacc ggctgatcca ggccgtacgc atgcttgagg tacgcgacgt ccgcttggtt
     1741 ggcgtcgggc ggcagcaggt tttgtaccgg atcgccggga gccagataga ccagacagaa
     1801 gcagatgatc gtcaccccga tggcgatcgg gatcgcgagc atgatccgcc ttgccgcgta
     1861 gtaccacatc gcgtcgtcac gactgcatcg ggcatgggca gccgagcaag taaggtgcca
     1921 aaaagccctt cccatggcgc aatctgccta cgctccgctg aacctgggag gaaccgcgcg
     1981 ggcaagcgcc ggaattcttg ccgccgtgcc caaaaatgcg gctgaacgcc cacacctgcg
     2041 gcgacgtgag gcgcagcgcc gccacgcctt caaaggcacg gtccttgcag gacaatttgc
     2101 aggaagagca gcagctctag cgcactcaac gccgagaggt gaccctcatg ctcgatctgc
     2161 acaaaacgtt tgcggcggcc gcccttatgg cagcgggcgt cctcgcgctc cagccgcaag
     2221 cggccagcgc ggaatcagtg ttgcgcatcg cgatgaccgc cggagacatc ccggactgga
     2281 cgggacagcc cgaccagggc ttcgagggct atcggttcgt cggctttgcc ctctatgacg
     2341 gcctcgtcaa ttgggatttg tcgcgcagcg atcgggaagc cgacatcaag cccgcgctgg
     2401 caaccaagtg gtccgccgat cccggcgatc tcaaaaagtg gatcatcgag ctgcgccatg
     2461 acgtcaagtt tcatgacggc tgcgattgga atgccgacgc cgcggtctgg aattttgagc
     2521 ggctgacatc gaaggcgagc cccgccttca gtccgctcca ctttgcccgc gcgcgctccc
     2581 gcagcaacga catcgatcgt gtcgagaaga tcgacgccta cacgatcgcg atccatacca
     2641 acgcgatcgt ctcgctgttt ccctataacc tgccattcct gctgatgatg tcgccctgcg
     2701 ccatggccca ggcgggcaac gactatcagg cctatgcgaa ggcgccggcc gggaccggcc
     2761 cgtacaagtt cgacaaggtc gtgccgcatc agcggctcga gctggtcaag aactccgatt
     2821 actgggaccg cggccgagtt cccaaacagg accgggtggt gctgttaccg atgccggagg
     2881 cttcgacgcg ggtcgcggcg ctgttgtccg gacagatcga ctgggtcgag gcgccgcccc
     2941 ccgacgcact cgaccgcctc aaggccgccg gcatgaacat cgtcaccaac gtctatccgc
     3001 acacctggcc ctatctgctg aacttcatgc gcgggccgtt cacggacctg cgggtgcgcc
     3061 aggcggcgaa ctacgcggtc aaccgtaacg agatggccga gatgctcaac ggcattgccg
     3121 tcccgagcta cggagtgttc gtgccgagcc agcgctatta cgggcatccg ttcgagcaca
     3181 agaccgaccc ggcgaaggcg acggcgctct tgaaagcggc cgggtgctac ccgtgtgcga
     3241 tcacggtcgc gatctcgacc tccggctcgg gtcagatgca gccgctgccg atgaacgaac
     3301 tcgtgaagga gcagctcgag aaggtcggat tcaaagtcgc gttcgagacg ctcgactgga
     3361 acaccatcct gaccatctat atccaaggcg cggtgaaata tccccaatat gacgccgtca
     3421 atttcagcag cggcgccacc gatccgctta actatctcaa aacggtcatg agcagctaca
     3481 aggcgcccaa cggggcaaac tggggcggct actccaaccc caaggtggat gagcttgcgc
     3541 tacgtgcgtt cgccaccttc gacgaagccg agcgcgacaa attggtcgcg cacatccacg
     3601 agaccaccag cgccgacgcc gtgcgcgtct tcatcgtcag cgacctcaat ccccgcgcgc
     3661 tgtcaccaaa gctgtcgggc ttcgtccagg ctcgcagctg gttccaggac atcacgcccg
     3721 tcgccgtcgg gaactgatgg ctgcggccgc tgaaggggct gtacgggtgc ggcagacaag
     3781 cgaagacgga cgagatccgg gtgaggggtc ttttcggtgc acaactcctt ccacccggac
     3841 actcgccgta gcgcatcgaa cggctcgcgt gaacgcgctt taggcgcact ctcaaatcac
     3901 ccggatgaac ggccgctcgc cggtcgggtc gacctccacc atcgcgccgt ttgcaatcgc
     3961 ctgcgtgatg tcgcggtcga aaccggagat catggtgaag tcggcgaacg ccgcgccctg
     4021 caccatgatc ggattggccg cgttcaacac cagcgccgcc ggcacgacgc cgcgcgcttt
     4081 catctcgcgc agcatccacg cggtggcaac gccgcccttg gcggcatcga gcacgagcac
     4141 gcggccgaca tagctctcgc ccgcgagctt gtggtcgggg cgcgaaaata cgccacgcag
     4201 gcgatcgaga tcatagcgcg cggagaagc
//
LOCUS       DATLJT010000286         4331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_934965,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000286 DATLJT010000000
VERSION     DATLJT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934965"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VM910_06440"
     CDS             complement(<1..1295)
                     /locus_tag="VM910_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HVI62215.1"
                     /translation="MPRHIWRRGRDLRRRLYETLEHGTIGDRTGLIVGRLIVALIITN
                     LVTMTLDSVPALEAQYGPLFIAVEVLSLIVFTIEYALRVWVAAEQAPHRHSSERKARW
                     EFISSPLGVIDLLAVLPFWFALVFPADLRVVLVFRMVRFLKLARYSTAMRSLLDALYS
                     ERRALFGCFVILLGATLLAASVMHVVEGHAQPDKFGTIPDAMWWAIVTLGTIGYGDVV
                     PVTVLGRIIASVTIFVGLIMVALPIGIVATAFADEIHRRDFVVTWGMVARVPLFAELQ
                     ATDIADIVRLLRAQRVEAGDVIAKRGEPAHSMYFVAAGEVEIELKHERVRLGAGHFFG
                     EVAALRRARRSATVTALTRTSLLVLDARDLHVLMERNRASRNTSAKWLALGWAATWSR
                     AKAIWSSKNSKKRRRSRDRRVATVGHDAKVNETTSNKPG"
     gene            complement(1335..1730)
                     /locus_tag="VM910_06445"
     CDS             complement(1335..1730)
                     /locus_tag="VM910_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62216.1"
                     /translation="MSLPHMLKRIRLELARSREFPAGSANHGYEFVAPLDSKGHIDPH
                     LWQKYREHCGVRRFWSGADEEIGRLAHKPGGAEHARWVFDYDPDTTDDGEAGYRFGVH
                     AFAPGEYVSIRSHEGGSHTFKVVSVEPAT"
     gene            complement(2151..3341)
                     /locus_tag="VM910_06450"
     CDS             complement(2151..3341)
                     /locus_tag="VM910_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62217.1"
                     /translation="MIPDSASAQAARLMMEFVSRTGLSPPARDHERYLWTDAFAVCNF
                     LELFRRTHDEENRRYAIQLIDQVHRVLGRYRDDDMRSGWISGHDEETARRHPTVRGLR
                     IGKPFKERDANEPVDERLEWDRDGQYFHYLAKWIHALCQAAFVTQEAACARWAVELGE
                     AAFEGFARRSHSGEVIGVYWKMSTDLSRPLVAATGLHDALDGFITLREARHAGKTLAN
                     VEATGLSAAIEALSVLCQHGNWITDDPLGLGGLLFDAGRLCQLPGEETLNDVRLLEEL
                     IGACHNGLIAFLAGRQLSRPASYRLAFRELGLATGLSALPMIADVISKDRTRFGNRPT
                     LGWTVHHLLPYSSLSDTIVSFWMPHAQHLDASWLAHQNINDVMLATALIPDTFLSIGR
                     PRLS"
     gene            complement(3485..3910)
                     /locus_tag="VM910_06455"
     CDS             complement(3485..3910)
                     /locus_tag="VM910_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVI62218.1"
                     /translation="MPVVIARHGELLHHGVCYMAEPSQHLMVGPGLRADLLPDHRYTT
                     RNIDQLFISLARHYGARTIGVVLSGQLDDGTRGLAAINKAGGIAMVQSPDEAAYPEMP
                     RNAIRYDGPIDLIAPISGLAAEIIHLTGTVPTKRLASIR"
     gene            complement(3898..4083)
                     /locus_tag="VM910_06460"
     CDS             complement(3898..4083)
                     /locus_tag="VM910_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVI62219.1"
                     /translation="MNAHPDIVAIGASSGGIEALQSLLAALSDLDAIVLVVLHRPANR
                     ISSLRARSCCESCGCRL"
     gene            complement(4116..>4331)
                     /locus_tag="VM910_06465"
     CDS             complement(4116..>4331)
                     /locus_tag="VM910_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVI62220.1"
                     /translation="APIALVDVHVRDGVVELWGMITDERERKAIVVAAENAPGVKAVN
                     DHLAWVDAISAMVLSPSREEPAQMKVS"
BASE COUNT          909 a         1351 c         1227 g          844 t
ORIGIN      
        1 aagcccggtt tgttgcttgt tgtctcattg accttcgcgt catgaccaac tgtggctacc
       61 cgccgatctc tggatcgtct ccgcttcttc gagttcttcg acgaccagat cgcctttgcg
      121 cgtgaccacg tcgccgccca gccgagtgcg agccacttcg cggatgtgtt ccgcgatgcg
      181 cggttccgct ccatgagcac gtgcagatcg cgggcatcga gcacgagcag gcttgtgcgc
      241 gtgagcgcgg taacggtcgc ggatctgcgc gcccgccgca gcgccgcgac ctcgccgaag
      301 aaatgaccag cgccgagccg aacgcgctcg tgcttgagtt cgatctccac ttctccggcg
      361 gcgacgaagt acatggaatg agcgggctcg ccgcgcttgg cgatgacgtc tccggcctcc
      421 acccgctgcg cgcgcaacag ccgcacgatg tcggcgatat cggtggcttg cagctcggcg
      481 aacaagggta cgcgcgcgac catgccccag gtcacgacaa agtcgcgacg gtggatctcg
      541 tcggcgaagg ccgtagcgac gatgccgatc ggcagcgcga ccatgatcaa tccaacgaag
      601 atcgtgacgg atgcgatgat gcgtcccagc acggtcaccg gaacgacgtc gccgtaaccg
      661 atggtgccga gcgtgacgat ggcccaccac atcgcatcag gaatggtgcc gaatttatca
      721 ggctgcgcat gaccctcgac cacgtgcatg acgctggcag cgagcagcgt cgcacccagt
      781 aaaatgacga agcagccgaa caaagcacgg cgctcgctat agagcgcatc caagagcgag
      841 cgcatcgcgg ttgagtagcg cgccaacttg agaaagcgca ccattcgaaa caccagtacg
      901 acccgcaggt cggccggaaa gaccaatgcg aaccaaaacg gcagaaccgc gagaagatca
      961 atcacgccga gcgggctcga aatgaactcc cagcgtgcct tgcgttcgct tgaatgccgg
     1021 tgcggcgcct gctcggccgc cacccaaacc cgcaatgcgt actcgatggt gaaaacaatg
     1081 agcgacaaca cttcgactgc gatgaatagc ggcccgtatt gcgcctccaa cgcaggcacc
     1141 gaatcgagcg tcatcgtgac gaggttcgtg atgatcagag cgacgatgag tcggccgacg
     1201 atgagacccg tccgatctcc gatcgtgccg tgctccaggg tctcatacag gcggcgtcga
     1261 agatctctgc ccctgcgcca gatgtgtcgc ggcatagctg agctctatcc aagcgatatg
     1321 ccatatcctc gctattacgt ggccggttca acggacacga ccttgaaggt atgggaccca
     1381 ccctcatgac tacggattga aacatactcg ccgggtgcga aggcgtgcac gccgaaacgg
     1441 tatccggcct cgccgtcgtc ggttgtgtcc gggtcgtagt cgaacaccca tcgcgcgtgc
     1501 tccgccccgc ccggcttgtg cgcgagtcgc ccgatttctt cgtccgcgcc gctccaaaac
     1561 ctgcgcaccc cgcaatgctc ccggtacttc tgccaaagat gggggtcgat atgccccttg
     1621 ctgtcgagcg gcgccacgaa ttcgtagcca tgattggcgg agcccgccgg aaactccctt
     1681 gatcgagcca gttcgagccg aatgcgtttg agcatgtggg gcaacgacat cggacaactc
     1741 cttcgcgggt gtggacctac tccaccgcta aagattgtca gagacagcga tcaccatttt
     1801 gatatttgtc aaatccagtc tcggtttctg ttcgtggtcc tggtttgagg cggtaaaagg
     1861 cgggagggca gacggtgcca ctaaaaggat cacacggggc ccgacaagcg caccgcccgc
     1921 cgccttgcct cgctccataa acaatagcgg tttgtcttcg cggcgctctc tcttgcgccc
     1981 gatccaaagc ttccgtctga cctatcttcc gctagtgacg gtctatttcg cctttggcgc
     2041 gcttgggctc atcgacatca ctagggacat ggatcaagga acaattgacg ttaagccccg
     2101 ccgagctcgc cagcaccctg actttgattg ccttgcgagg cgctccctat ttaggagagc
     2161 cgcggtcgac caatcgacaa aaaggtgtcc ggtatcaatg cggtcgcaag catgacgtca
     2221 ttgatatttt ggtgagctag ccagctggcg tcaaggtgct gtgcgtgcgg catccagaaa
     2281 ctgacgatcg tgtcacttaa cgagctgtac ggtagaaggt gatgaacggt ccatcctaac
     2341 gtaggcctgt tcccaaacct ggttctgtct ttcgaaataa catcggcaat cataggcaat
     2401 gcgctcaggc ctgtggctaa gccaagttct cggaaggcca agcggtacga ggcaggtcgg
     2461 cttagctgcc gaccggcgag aaaggcgatc aaaccgttat ggcaggcgcc tatcaactct
     2521 tccagaagac gaacatcgtt caatgtctcc tcgccgggta actgacaaag acggccagca
     2581 tcgaacagca gcccaccaag tccgagcgga tcgtccgtta tccaattccc atgctgacat
     2641 agcacggata gtgcttcaat cgccgcgcta agaccagtcg cctcgacgtt ggccagagtc
     2701 ttccctgcat gccgcgcttc ccgcaaggtg atgaaaccat ccaatgcgtc atgcaacccc
     2761 gtggctgcaa cgagcggtct tgaaagatcg gtgctcatct tccaatagac cccaatcacc
     2821 tcgcccgagt gggaccggcg ggcaaagcct tcgaatgctg cctctcctag ttcaaccgcc
     2881 caacgagcac acgcggcctc ttgggtgacg aaggcggctt ggcagagggc atgtatccac
     2941 ttggccagat agtggaagta ctgaccgtcg cgatcccact ccagacgttc gtcaactggt
     3001 tcgttcgcgt cgcgctcctt gaacggtttg ccgatcctca gccccctcac cgtgggatgg
     3061 cgacgcgctg tctcttcatc gtgtccgctg atccacccgc tccgcatatc gtcatcgcga
     3121 tatcgcccga gcacccgatg cacctgatca attaactgta ttgcgtatcg ccgattctct
     3181 tcatcgtgtg ttcgccggaa aagctccaga aagttgcaga ctgcaaacgc atcggtccaa
     3241 agatatcgtt catgatcccg cgcaggtgga cttaagccgg ttcggctcac gaattccatc
     3301 atcaatcgag ccgcttgggc agatgcactg tccgggatca tttcgcaatc acaaatagga
     3361 tacgggctgc gagtggcgct agccgccgtc tgaaaaatga gtcttcccgg acatagatta
     3421 accggtgaat ggcgttgcga cacgaccgcc cttatgacta cggcctcgct ttctgaacaa
     3481 tcgtttatcg gatcgacgcc agccgcttgg tgggaaccgt accggtcagg tggataatct
     3541 ccgcggcgag acccgaaatc ggcgcgatca gatcgatcgg cccatcatat ctgatcgcgt
     3601 tgcgaggcat ttccggatat gcggcttcgt ccgggctctg gaccatggca attccgccgg
     3661 ctttgttgat ggcggcgagc ccgcgcgttc cgtcatcgag ctgccccgaa agcaccacgc
     3721 cgatggttct tgccccgtaa tgccgcgcca gcgaaatgaa cagctgatcg atattgcggg
     3781 tggtgtagcg atggtcgggc agcaggtcgg cgcgcagacc aggtccaacc atcagatgct
     3841 gtgaaggctc cgccatgtag cagacgccgt gatgcagcag ctctccgtgt cttgcgatca
     3901 caaccggcat ccgcaactct cgcagcagga tctcgcgcgc aaagagctga tgcgattggc
     3961 cggccggtgc aacacgacca acacgatggc atcgagatcg gaaagcgctg cgagaaggct
     4021 ctgcaatgcc tcaatcccgc cgctcgaggc gccgatggcg acgatgtcgg gatgagcgtt
     4081 catcgaccaa gaccgtcaag cgttgccgaa aaaagtcagg acactttcat ctgcgccggc
     4141 tcttcacgcg atggggacag caccatggcg gatatggcat cgacccaggc caaatgatcg
     4201 ttgaccgcct tcacgcccgg cgcgttctcc gccgccacca caatcgcctt gcgctctcgt
     4261 tcgtccgtga tcatgcccca gagctcgacg acgccatccc tcacatgcac atcgacgagg
     4321 gcaatcggcg c
//
LOCUS       DATLJT010000287         8905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4151789,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000287 DATLJT010000000
VERSION     DATLJT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4151789"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..669
                     /locus_tag="VM910_06470"
     CDS             298..669
                     /locus_tag="VM910_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62221.1"
                     /translation="MSTDFSIRPVGAPAPSPVVQPFSQAASNAVATDLPASQSVTAAD
                     ASTVVRNDPQSPVDDAFVSHQAYFDRAAASMVYQVVDSKTDQVVEQYPDEAVLRRRAY
                     FHALDLAKGTPPRVIPTDLVA"
     gene            complement(710..1075)
                     /gene="flaF"
                     /locus_tag="VM910_06475"
     CDS             complement(710..1075)
                     /gene="flaF"
                     /locus_tag="VM910_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HVI62222.1"
                     /translation="MSNAAQAYARISQTTATPREIEAQALLKAARQLQEVQANWNGPD
                     KNLHKALLFNRRLWSIFMTAAEANDNPNSLEVRQNIANIGVFVLKQTIEMQMDPHPAK
                     LKTLIDINCNIAAGLSGRA"
     gene            complement(1272..2867)
                     /locus_tag="VM910_06480"
     CDS             complement(1272..2867)
                     /locus_tag="VM910_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVI62223.1"
                     /translation="MSGIVLSASVRQNLLALQSTASLLATTQNDLATGNKVNTALDNP
                     TEFFTAQSLNNRASDIANLLDGIGNGVQVLQAANTGLTSLSALVANAQSIANQVLQSP
                     TGYDTKSNVISTAIAGANANNLLGTYQNNTTTGGVIQNDNSAGAQPITTATQLVGTAA
                     SGSNDLATAITSGSILTIDGKTITFTTSQTTVTSDGQNDYVFGIGTGSTLKVSDVLTA
                     IDSITGGSSTPSTVTNGKLVLSTGTSQNLVIGGSGNALAALGLTAGTTSLTPPALSGQ
                     TLTIAATGGGTATNITFGTATGEISTLNQLNTALSANNLQASIDTTGRISITTTNDAA
                     SSTMGPIGGSAVPFSGLSAAAPAVDPNSQAQRANLINQYNNVLAQINTTAQDASFNGI
                     NLLNGDTLKLVFDETGRSTLNIQGVTFNDAGLGLSNLAVGIDFLDSNSANKVLTSLDS
                     AATTLRSEASALGSNLSIVQIRQDFNKNLINVLQTGASNLTLADTNEEAANSQALSTR
                     QSIAVSALALANQSQASVLQLLR"
     gene            3161..3586
                     /gene="flbT"
                     /locus_tag="VM910_06485"
     CDS             3161..3586
                     /gene="flbT"
                     /locus_tag="VM910_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HVI62224.1"
                     /translation="MTLKVELKPHERIIIGNCVVTNTDQRTRLLIDGDNIPILREKDI
                     LTPETADTPAKLIYLAVQLMYISPDARANHGTYFNLIRDLVTAVPSVWPIIEAINNHI
                     LSGDLYQALKECKKLVAYERKLLDQIEALHAKGAVSTAA"
     gene            complement(3631..5124)
                     /locus_tag="VM910_06490"
     CDS             complement(3631..5124)
                     /locus_tag="VM910_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVI62225.1"
                     /translation="MSSTPREAALRAMPGETLDPQDWDEIRAQGHRMLDDMIDYIATI
                     RERPVWRPIPDVVRAQFREDLPREPTHLDEVYRTFTDFIVPYTTGNVHPGFMGWVHGG
                     GSPVGMLAEMLAAGLNANLGGRDHIPIEVERQIVEWTRQMFGFPDSASGIFVTGTSMA
                     NLMAVLVARISALGTSARRQGIGQDGTLLTAYTSMAAHGCITRAMDIAGFGSDALRCI
                     AVDRAHRIDVAALRAQIARDRAAGRKPFLVVGSAGTVDVGAIDDLSALASLCREEALW
                     FHVDGAFGALGVLSPVLAPKLAGIEQADSIALDFHKWGQVPYDAGFLLVRDGERHRDA
                     FAAPAAYLRREARGLAAGSPWPCDLGPDLSRGFRALKTWFTLKTYGIAKLGAVISRCC
                     ALAGYLEARVKAEPRLELLAPVNLNIVCFRYRAADADRVNGEIVVNIQESGIAAPSTT
                     VLDGALAIRAAIVNHRTDIGDIDALVEAVLEFGARRSAGRDRLSAIA"
     gene            complement(5121..6311)
                     /locus_tag="VM910_06495"
     CDS             complement(5121..6311)
                     /locus_tag="VM910_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62226.1"
                     /translation="MARLAKMAFDGKDLRPLWGELVRKVFDGSADAGEGLDLSLIAQL
                     LGDKQSGLAIQHEVLLSHQLFRSPCASNNPRLRVLALAAATDMGSNTPIEFLLEEFGI
                     ELMVLYVIPEAGLPMPLPDHDIAIVIASDSEDCRDALRRIDTLAPRWPRPLLNPPAGI
                     GILDRDKLHRLLDGIDGLNIPATRVVTRERLSEVAGGADALRDIAVDLAFPVIVRPRG
                     SHAGTGLARIDDAADMARHLEGRDEELFFISRFVDYASEDGLFRKYRVVFVEGRPYAC
                     HMAIADRWDIWYLNAGMSASAAKRLEEEAFMRTFDIGFGRRHQAALAAMFERIGLDYF
                     TIDCAETRDGSLLIFEADNTAVVHNMDSPQIFPYKPPQMRKIFGAFAAMLERRVGISR
                     ERAA"
     gene            complement(6364..8004)
                     /locus_tag="VM910_06500"
     CDS             complement(6364..8004)
                     /locus_tag="VM910_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62227.1"
                     /translation="MNPAAPRSQIAETLLFRRPERARAPALSDLFMPLDDLLVRGGDP
                     RLMLDCRGGINEYGCGPFPSAETWSFASSTASPISERAYERAGLAREKLMRSAIAVGF
                     DEALDVRIEEMREELRSCLRLSAADVDVVFSPSGTDSQLHALFLARCLVGSQLTTVVV
                     GSDQTGSGTAFTARGRHFSSITPSERIVRKDTPIAGLSCDSVALPLIEGTTDIRARAN
                     GDLAVLDAIEAAVANGASVLLQIMDSSKLGWRAPSVACLDEIASRWPDEVLVVVDACQ
                     MRLSRRRIRTYLDRSYIVLITGSKFFGGPAFSGALLVPAGLARSLDRGDSVPSGILDY
                     ASRSDWPKGFAALRSCFENRANIGQWLRWEGALEEIKAYYDVPDEYRALALRELRIGM
                     ESLIALSPSLRPLPACAKSHAAEDEEFSAATIFPFTLHRGGDALSASDCRIVHRALAR
                     DLGDIVAGSEADRELAARRCLVGQPVRIERHGEPCTAALRLCLGARLVTETWSSDPRV
                     AQQNLQRELDRIATVVAKIELLLARAGVPDLSEPDYGI"
     gene            complement(8017..8796)
                     /locus_tag="VM910_06505"
     CDS             complement(8017..8796)
                     /locus_tag="VM910_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62228.1"
                     /translation="MTDRVHLVVATPCFGGQVSSIYASSIFALQRALRSVSNIDLTVH
                     MRDGDALITRARANLVTLFLDDPAATHLLFVDADIGFEPKQVFRLIESGADVVAGCYP
                     IKRINWDKARRALEAGRINPKAASLDYVLEIDNPDHVVVVNGFTRVRYAGTGFLMIRR
                     HVLERMCAHPDYAPLRFLREHSLDALAGSPNRFALFECMIDPATGAYLSEDFAFCKRW
                     TDIGGEIWADLDSRLDHVGPSVFHGDVASQFAAAPTAADAA"
BASE COUNT         1712 a         2745 c         2818 g         1630 t
ORIGIN      
        1 cgtgtcggag aacatcgtgc ggggcgtcaa cgccgagatg cagcgccgca acatccccaa
       61 cacctacacc gcggccggac ggcgcgccgc gccggggccg cggcacatca cgccgctgtc
      121 ggtcagccgc tcgctctgaa ccgcgccccg aaataccgac aattttcatt aaatttgcgc
      181 gcctgcctaa gcgcgccgtt catcgcgttt tctaacgtga cgttgagagc tgactgtcat
      241 tgttgcgggc ggagactggt tgggatttga ctctgcgagc ggcaccagga ggctgccatg
      301 agcacggatt tcagcatcag gccggtgggg gcgccggctc cctcgcctgt tgtacagccg
      361 ttcagccagg cggcgagcaa cgcggttgcg accgatttgc ctgcaagcca gagcgtcacc
      421 gctgcggacg ccagcacggt cgtgcgcaat gatccgcaaa gccccgtcga cgacgcattc
      481 gtttcgcacc aggcatattt cgatcgcgct gcggcctcga tggtctatca ggtcgtcgac
      541 agcaagactg atcaggtcgt cgagcaatat cccgacgaag cggtgttgcg ccgccgcgcc
      601 tatttccacg cgcttgacct cgccaagggg acaccgccgc gcgtgatccc gaccgatctc
      661 gtggcttgaa gcaagcgagg cggattccct atctgtgcgg tgaacgatat caggcgcggc
      721 cggagagtcc cgccgcgata ttgcaattga tgtcgatcag ggttttcagt ttcgcagggt
      781 gcggatccat ctgcatttcg atggtctgct tcagcacgaa cacgccgata ttggcgatgt
      841 tctggcgaac ctccaacgag ttcgggttgt cgttggcctc cgccgcagtc atgaaaatcg
      901 accacaaccg gcgattgaac agcagcgcct tgtgcaggtt cttgtccggg ccgttccagt
      961 tggcctgaac ttcctgaagc tgtcttgccg ccttgagaag ggcctgcgct tctatttccc
     1021 ggggagttgc cgtcgtttga gagatacgtg cataggcttg agcggcatta gacatccgtt
     1081 accccggatt acgctgagat gcgacagaat tgctcagttt ttctacgtgg ttgcctttaa
     1141 aatatggtta gcgactgttt cagtaaacgc gctgcgaata ttaaatcgtc gtcaaagctg
     1201 aaatgcgtgt ggcgcaaaac aaaacggcgg gaccgccccg ccgctttgcc ctttgtgctt
     1261 gctcgtcgat atcagcgcag gagctgcaac acgctcgcct gcgactggtt ggcgagcgcc
     1321 agcgccgaga ccgcgatcga ctggcgcgtc gacagcgcct ggctgttggc ggcttcctcg
     1381 ttggtgtcgg ccagcgtcag attcgatgcg ccggtttgca gcacgttgat caggtttttg
     1441 ttgaagtcct gacggatctg cacgatcgag aggttcgaac ccagcgcaga agcctccgaa
     1501 cgcagcgtag ttgcggcgct gtcaagcgaa gtcagcacct tgttcgcgga gttgctgtcc
     1561 aggaagtcga tgccaaccgc caagttcgag agtccgagcc cggcgtcgtt gaaagtcacg
     1621 ccctggatgt tgagggtcga acgtccggtc tcatcgaata cgagcttcaa cgtgtcgccg
     1681 ttcagcaggt tgataccatt gaaggaggcg tcctgcgcag tggtgttaat ctgggccagc
     1741 acattgttgt actgattgat cagatttgcg cgttgcgcct gcgaattcgg atccacagcc
     1801 ggagcagcag ccgacagacc gctgaaaggc actgcggaac cgccgatggg gcccatcgtc
     1861 gaggacgccg catcattcgt ggtcgtgatg ctgatcctgc cggtggtgtc gatgctcgcc
     1921 tgcaggttgt tcgccgacag cgccgtgttg agctggttca gcgtggagat ctcgccggtg
     1981 gcggttccga aggtgatgtt ggtcgcggtg ccgccgccgg ttgcggcgat ggtcagcgtc
     2041 tggcccgaaa gtgccggcgg cgtcaggctg gtggtgccgg cggtgaggcc gagtgctgca
     2101 agcgcgttgc ctgaacctcc aatcaccagg ttctggctgg tgccagtcga cagcaccagc
     2161 ttgccattgg tgaccgtgga aggagtcgaa gagcccccgg tgattgagtc gattgcagtc
     2221 agaacatcgc tgaccttaag cgtcgaaccc gtgccgatgc cgaagacata gtcattctgt
     2281 ccgtcgctcg taacagtcgt ctgcgacgtc gtgaaggtga tggtcttgcc gtcgatcgtc
     2341 agaattgagc cgctcgtgat ggcggtcgcg aggtcgttgg agccgctggc cgcggtgccg
     2401 accaactggg tcgcggtggt gatcggctgc gcgccggcgc tgttgtcgtt ttggatgacg
     2461 ccaccagtag tagtgttgtt ctggtacgtg ccgagcaggt tattcgcgtt cgcgccggcg
     2521 atggcagtcg agatcacgtt cgatttggta tcgtagccgg tcgggctttg cagcacctgg
     2581 ttggcgatcg attgggcgtt tgcgaccagg gcactcagtg aggtgagccc ggtatttgcc
     2641 gcctgcagca cctgcacgcc gttgccaatg ccgtcaagca ggttggcgat gtcgctcgca
     2701 cggttattga gcgactgggc cgtgaagaat tcggtcgggt tgtcgagagc cgtgttgacc
     2761 ttgttcccgg tagcgaggtc gttttgtgtc gtcgcgagca acgaagcggt cgactgcaat
     2821 gcgagcaggt tctggcgaac cgatgccgaa agaacaatgc cggacatgtg tcaaaccttc
     2881 ctagttaatt gcttcatttc taggggagac acgtttaaag tatggttaat ggcggtttaa
     2941 attattgggt taacgattcc ttacctgccc tttccggcgc tgaatgcgat gttactgagg
     3001 tctagaaacg caaaagcgcc cgggattccc ctttaccaag cattaacctt gatcggaaag
     3061 gatcgcccca gacgctgacc gggggcatcc ggcgaagcca gtgtgttcga aaattcgcgg
     3121 ctattgatcg ccggcgtctg aagcggccat ggaggacaat atgactttga aagtcgaact
     3181 gaagccgcat gaacgaatca tcatcggcaa ttgcgtggtc accaacaccg atcaacgcac
     3241 gcgacttctg attgacggcg acaacattcc cattctgcgc gaaaaagaca ttctcacgcc
     3301 ggagaccgcc gacacgccgg ccaaactgat ctatctggcg gtgcaattga tgtatatctc
     3361 gccggacgcg cgggccaatc acggcaccta tttcaatctg atccgcgatc tcgtcaccgc
     3421 cgtgccgagc gtctggccga tcatcgaggc catcaacaac catattttga gcggtgacct
     3481 gtaccaggcg ctgaaggaat gcaagaagct ggtggcttac gagaggaagc tgctggatca
     3541 gatcgaagcg cttcacgcca agggcgcggt cagcaccgcc gcctgagccg ggcgatttgc
     3601 ggcgcgttcc cgcggatatc cgcgcgccga tcaggcgatc gcgctcaacc gatcgcgtcc
     3661 ggccgaccgc cgcgctccga attcaagcac cgcctcgacc agcgcgtcga tgtcgccgat
     3721 atcggtgcgg tgattgacaa tggcagcccg gatcgcaagc gcgccgtcca atacggtcgt
     3781 cgacggcgcc gcgatcccgg actcctggat attgacgacg atctcgccgt tgaccctgtc
     3841 tgcgtcggcg gcgcgatagc ggaaacagac gatgttgagg ttgacaggtg cgagcaattc
     3901 cagccgcggt tcggccttca cgcgcgcctc cagataaccg gcgagtgcgc aacagcggct
     3961 gatgaccgcg ccgagcttcg cgatgccgta ggtcttcagg gtgaaccagg tcttgagcgc
     4021 cctgaacccg cgcgacaaat ccggaccaag gtcgcaaggc cacggcgaac ccgcagcaag
     4081 tcctcgcgcc tcgcgcctca aataagccgc cggcgcggca aaggcgtcgc gatgccgctc
     4141 gccgtcgcgc accaggagaa agccggcgtc gtagggcacc tggccccatt tgtggaaatc
     4201 cagcgcaatc gaatccgcct gctcgatgcc tgcaagcttc ggcgcgagca cgggtgaaag
     4261 aacgccgagc gcgccgaacg cgccgtcgac atggaaccag agggcttcct cccggcatag
     4321 cgaagcgagc gcggaaagat cgtcgatcgc accgacatcc acggtcccgg cggatccaac
     4381 caccaggaaa ggctttcgtc cggctgcgcg gtcgcgcgcg atttgcgcgc gcaatgccgc
     4441 cacatcgatg cgatgggccc gatcgacagc gatgcatcgt aacgcgtcgc tgccgaaacc
     4501 ggcgatgtcc atcgctctgg tgatgcagcc atgcgcggcc atcgacgtat aggccgtgag
     4561 cagcgtgccg tcctggccga tgccttgccg gcgggcggat gttccgagcg ccgagatgcg
     4621 cgccaccagc acggccatca gattggccat cgacgtgccg gtgacgaaaa tgccgctggc
     4681 cgagtccgga aatccgaaca tctggcgcgt ccattcgacg atctggcgtt cgacttcgat
     4741 gggaatatga tcgcgtccgc cgagattggc gttgagcccg gccgccagca tttcggccag
     4801 catgccgacc ggcgagccgc cgccatgcac ccagcccatg aagcccggat gaacgttgcc
     4861 cgtcgtatag ggcacgatga agtccgtgaa ggtgcgatag acctcgtcga gatgcgtcgg
     4921 ctcgcgcggc agatcttcgc ggaactgggc gcgcactacg tcgggtatcg gccgccatac
     4981 cggacgctcg cggatcgtgg cgatatagtc gatcatgtca tcgagcatgc gatgaccttg
     5041 cgcgcggatc tcgtcccaat cttgcggatc gagcgtctcg cccggcatcg cacgcagcgc
     5101 ggcttcgcgc ggcgtgctgc tcatgccgcc cgctcccgcg aaattccgac gcggcgctcc
     5161 agcatggcgg cgaatgcgcc gaagatcttg cgcatctgcg gcggcttgta gggaaagatt
     5221 tgcggcgaat ccatgttgtg gacgaccgcc gtattgtcgg cctcgaagat caggagcgag
     5281 ccgtccctgg tctccgcgca atcgatggtg aagtagtcga gcccgatacg ctcgaacatc
     5341 gccgcaagcg ccgcctggtg acggcggccg aagccgatgt cgaaggtccg catgaacgcc
     5401 tcttcctcga gacgcttggc tgcgctcgcc gacatgccgg cgttgagata ccagatgtcc
     5461 cagcggtcgg cgatggccat gtggcaggca taaggccggc cttcgacgaa gacgacccgg
     5521 tacttgcgga aaagaccatc ctcgctcgcg tagtcgacga aacgcgaaat gaagaacagt
     5581 tcctcgtcgc gtccctcgag atggcgcgcc atatcggcgg catcgtcgat cctggcaagt
     5641 cctgtgccgg catgcgatcc gcgcggccga acgatgacgg ggaaagcgag atcgactgcg
     5701 atatccctca gcgcatccgc cccgccagcc acctccgaca agcgctcgcg cgtgacgacg
     5761 cgtgttgcgg gaatattgag accgtcgatg ccgtcgagca gacgatgcag cttgtcgcga
     5821 tcgagaatgc ctatgccggc cggcggattg agcaacggac gcggccagcg cggcgccagc
     5881 gtatcgatcc tgcgcagtgc gtcgcggcag tcctcggaat cggaggcaat cacgattgcg
     5941 atgtcatgat ccggcagcgg catcggcaac ccggcctcgg ggatgacgta cagcaccatc
     6001 agctcgatgc cgaactcctc cagcaaaaac tcgatcggcg tattgctgcc catgtcggtt
     6061 gccgccgcga gcgccaaaac tcgcagacgc ggattgttcg acgcgcaggg cgaacggaac
     6121 aactggtggg acagcaacac ctcgtgctgg atggcgagcc ctgactgctt gtcccccaga
     6181 agctgggcga tcagcgacag atcaagcccc tcgcccgcat ccgccgagcc gtcgaacact
     6241 ttcctgacaa gctcgcccca cagcggacga agatccttgc cgtcgaacgc catcttcgca
     6301 agcctggcca tgccgatgcg gtcggcgcaa cgggcgcggg cggcgtcggc gatagcgagc
     6361 ggttcaaatt ccatagtcag gctccgataa gtccggtacg ccggcgcgag cgagcaagag
     6421 ctcgattttg gcgacgacgg ttgcgatgcg atcgagctcg cgctgcagat tttgctgcgc
     6481 gacgcgcggg tctgacgacc aggtttcggt tacgaggcgc gcgccgaggc acaggcgcag
     6541 cgccgctgtg catggctccc cgtgccgctc gatgcgaacg ggttgaccga caaggcaacg
     6601 gcgcgcggca agctcgcgat ccgcctcgct gcctgcaacg atgtcgccga ggtcgcgcgc
     6661 cagagcgcgg tgcacgatcc tgcaatccga cgccgagagc gcatccccgc cgcggtgaag
     6721 cgtgaatgga aagatggtgg cggctgaaaa ttcttcgtcc tcggccgcat ggctcttcgc
     6781 gcaggccggc agcgggcgaa gcgacggcga cagcgcaatc aggctttcca ttccgatcct
     6841 gagctcgcgc agcgccagcg cacggtactc gtccggcaca tcataatagg ccttgatctc
     6901 ctctagcgcc ccttcccatc gcagccattg accgatgttc gcgcggttct cgaagcacga
     6961 ccgcaacgcc gcaaatccct tcggccaatc gctacggctc gcataatcga gaatccctga
     7021 ggggacgctg tcgccgcggt cgagcgagcg cgccaaacct gccggcacca gcagcgcgcc
     7081 gctgaaggcc gggccgccaa agaatttcga gccggtgatc agcacgatgt aactgcgatc
     7141 gagataggtc ctgatccggc gccggctcag tcgcatctgg cacgcgtcga ccacgaccag
     7201 aacctcgtcc ggccatcgcg aagcgatttc gtcgagacag gccacgctcg gcgcgcgcca
     7261 gccgagcttc gaggaatcca tgatctgcaa cagcacgctc gcgccattgg ccaccgcagc
     7321 ttcgatcgca tcgagaaccg cgagatcgcc attggcgcgt gccctgatat ccgtcgtccc
     7381 ctcgatcagc ggcagcgcca cgctgtcgca ggacaggccg gcgatcggcg tatccttgcg
     7441 gacgatccgc tcgcttggcg tgatcgaact gaagtgtcgg ccgcgcgccg taaaggccgt
     7501 gccgctgccg gtctggtccg agccgacgac gacggtcgtg agctgggagc cgaccaagca
     7561 gcgcgcgaga aacagggcat gaagttgcga atcggtcccc gagggcgaga agaccacatc
     7621 cacgtcggcc gccgacagcc gcaggcagct tctcagctcc tcgcgcattt cctcgatgcg
     7681 cacatcgagc gcctcgtcga atccgaccgc aatggccgag cgcatcagct tctcgcgtgc
     7741 aagcccggcg cgctcgtagg cccgctccga gatcggtgac gccgtgctcg aggcgaagct
     7801 ccaggtttcg gccgacggaa acggtccgca accatattcg ttgatgccgc cgcggcagtc
     7861 gagcatgagc cgcggatcgc cgccgcgaac gagcaaatcg tcgagcggca tgaacaggtc
     7921 ggacagtgcg ggagctcgcg cgcgctccgg acgccggaag agaagggttt cggcgatctg
     7981 cgatctcgga gcggcgggat tcatttgcgc tgttctttaa gcggcgtccg ccgcagtcgg
     8041 agccgccgcg aattgagagg caacatcgcc gtggaagacc gacggcccga catggtcgag
     8101 ccggctgtcg aggtcggccc agatctcgcc gccgatgtcg gtccagcgtt tgcagaaggc
     8161 aaaatcctcg ctgagatagg cgccggttgc cggatcgatc atgcattcga acagggcaaa
     8221 ccggttcggg ctgcccgcca gcgcgtccag cgaatgctcg cgcagaaatc gcagcggcgc
     8281 gtagtccgga tgcgcgcaca tcctttccag gacatggcga cggatcatca aaaagccggt
     8341 gccggcatag cgcacgcggg taaagccgtt gaccacgacg acatggtcgg gattgtcgat
     8401 ctcaagcacg tagtcgagcg aagcggcctt cgggttgatc cggcccgctt ccagcgcccg
     8461 cctcgccttg tcccagttga tgcgcttgat cggatagcag ccggcgacga catcggcgcc
     8521 ggattcgatc aagcggaaga cctgtttggg ctcaaagccg atatccgcgt ccacgaaaag
     8581 cagatgggtg gctgcgggat cgtcgaggaa caacgtgacc aggttggcgc gggcgcgggt
     8641 gatcagcgca tcgccatcac gcatgtgaac cgtcagatcg atgttggaca cggaccgcaa
     8701 cgcccgctgc agggcgaaga tcgagctggc atagatgctg gatacctgcc cgccaaaaca
     8761 gggcgtggcc acaacaaggt gaacgcggtc ggtcatcaac ggctccgaag cgacaggcaa
     8821 cggcctcatt tctgaccaaa ccgtaaatag atgtggttaa cgaaaaccta acgcccctgc
     8881 ccgatcaggc cagcggcagg aactt
//
LOCUS       DATLJT010000288         4126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2773288,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000288 DATLJT010000000
VERSION     DATLJT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773288"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VM910_06510"
     CDS             <1..343
                     /locus_tag="VM910_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HVI62229.1"
                     /translation="TRKIYDTIADNAANAGIVLGGRPIRPLEADLRWIGALCFRNGQL
                     EETGLAAGVLNHPATAVAWLANKIAPLGLALEPGQVVLAGSFIRPIETRKGDTIQADY
                     GAYGSVSCYFA"
     gene            368..766
                     /locus_tag="VM910_06515"
     CDS             368..766
                     /locus_tag="VM910_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HVI62230.1"
                     /translation="MPHFTIEYSANLDERLDMAEVVDVVRKAAVETGIFPLGGIRVRA
                     VRCEHYAIADGRPAYGFLDMVLRLGEGRDLATRKKAGEHIFKALSAHLDPVFAQSKFA
                     LSFDMQINDKETSWKRNNIHDALKAEAVHG"
     gene            759..2297
                     /gene="hpaE"
                     /locus_tag="VM910_06520"
     CDS             759..2297
                     /gene="hpaE"
                     /locus_tag="VM910_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HVI62231.1"
                     /translation="MDKATPKSDLFKANLDRAAPLLQQLRADGIGHMIDGKIVPSISG
                     ETFDTKSPIDGAVLATVARGNAEDIDRAATAAALAFKSWRDMSASMRRKLLHRVADAI
                     EDRADDIAVLECIDTGQAHRFMAKAAIRAAENFRFFAGKCAEARDGLNMPSEEHWNIS
                     TRVPIGPVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPVTADLLAKLVKEAG
                     VPDGVLNTVHGFGEEAGKALTEHPAIKAIGFVGESTTGSAIMAQGAPTLKRVHFELGG
                     KNPVIVFDDADLDRALDAVVFMIYSLNGERCTSSSRLLIQQSIAEKFMEKLAVRVKAL
                     KVGHPPDPATEIGPLIHERHLAKVCSYFDVARKDGAVIAVGGKAHDGPGGGHYVEPTL
                     VIGAHTKMRVAQEEVFGPFLTAIPFRDEADAIEIANDVNYGLTGYVWTGDMGRALRVA
                     DALEAGMIWLNSENVRHLPTPFGGMKASGIGRDGGAYSFDFYMETKHVSLARGTHRIQ
                     RLGI"
     gene            2421..3404
                     /gene="hpaD"
                     /locus_tag="VM910_06525"
     CDS             2421..3404
                     /gene="hpaD"
                     /locus_tag="VM910_06525"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HVI62232.1"
                     /translation="MPVPQHVFDPPFNIIRSSHAVLDVTDLKLSREFYETTVGLHVED
                     SDDDAVYFRGSEEHQHHSLVLRKAAVPACNRLGFKVGNEQDLDKAAAFFSSNGITYAF
                     AEPPFQGRTLQFTDPFGFRIELYATMEKRPHLLRRYDLYRGCHPQRIDHFNVFAAEVQ
                     DTVDFYARLGFRLTEYAEEDGPNGRMAAAWLHRKGNVHDFALTNGNGPRLHHFAYWVP
                     TAMNILHLCDVMASSGYLKNIERGPGRHGISNAFFLYIRDPDGHRLELYTSDYFTMDH
                     DHAPMRWSLKDPRRQTLWGAPAPRSWFEQGSPFTGQKVREPLFVADVTVAD"
     gene            3414..>4126
                     /locus_tag="VM910_06530"
     CDS             3414..>4126
                     /locus_tag="VM910_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI62233.1"
                     /translation="MTSPRLVTYSIRGSTHYGAIVDGGVADLSARFGKDYPTLREVIA
                     AGALLKLADGAAGRSPDHALDAIFFQPPIPAPEKIICIGVNYPDRNAEYKDGSDAPKY
                     PSMFMRTPRSFVGHDTPLVRPHASQQLDYEGELAIVIGKPGRHIGESQALDHIAAITL
                     CNEGTIRDWVRHAKFNVTQGKNFDATGSLGPWIVPYTSEAQIADIRLTTRVNGETRQD
                     DRTSRLIFPFRYLINYIST"
BASE COUNT          799 a         1350 c         1224 g          753 t
ORIGIN      
        1 gacgcggaaa atttacgaca ccatcgccga caacgcggcg aatgccggca tcgtgctcgg
       61 cggacggccg atccgtccgc tcgaggccga tctgcgctgg atcggcgcgc tctgtttccg
      121 caacgggcaa ttggaagaga caggccttgc cgccggcgtg ctcaaccatc cggctaccgc
      181 cgtcgcctgg cttgccaaca aaatcgcacc gcttgggctt gcactcgaac ccggacaggt
      241 ggttctggct ggatcgttca tccgcccgat cgagacccgc aagggcgaca caatccaggc
      301 cgactatggc gcctacggtt cggtcagttg ttatttcgca tagaactctg acgtggagcg
      361 atccgttatg ccgcatttca cgatcgaata ttccgccaat ctcgatgaac gcctcgacat
      421 ggccgaggtc gtcgatgtcg tccgcaaggc ggccgtggag accggcattt tcccgctcgg
      481 cggcatccgc gtgcgcgccg ttcgctgcga gcactacgca atcgcagacg gccggccagc
      541 ttacggcttt ctcgatatgg tgctgcggct tggtgaaggc cgggatctcg ccacccgcaa
      601 gaaagccggt gagcatatct tcaaggcact atccgcccat ctcgatccgg tcttcgccca
      661 aagcaaattt gcgctgtcgt tcgacatgca gatcaacgac aaggagacga gttggaaacg
      721 caacaacatt cacgatgcat tgaaggcgga ggctgtccat ggataaggcc acgcccaaat
      781 ccgatctgtt caaggccaat ctggaccgcg ctgcgccact tttgcagcaa ctgcgcgccg
      841 acggcatcgg tcacatgatc gacggcaaga tcgtgccgtc gatctccggc gagaccttcg
      901 acaccaaatc gccgatcgac ggcgcggtgc tggcgacggt tgcccgcggc aatgccgagg
      961 atatcgaccg cgccgcaacc gcagccgcgc ttgccttcaa atcctggcgc gacatgtcgg
     1021 cgtcgatgcg ccgcaaactg ctgcatcgcg tggccgacgc gatcgaggat cgggccgacg
     1081 acatcgccgt gctcgaatgc atcgacaccg gacaggcgca tcgcttcatg gccaaggccg
     1141 cgatccgcgc tgccgagaac ttccgcttct ttgccggcaa atgcgcggag gccagagacg
     1201 gcctcaacat gccgagcgag gaacactgga acatttccac gcgcgtgccg atcgggccgg
     1261 tcggcgtgat cacgccgtgg aacacgccgt tcatgctgtc gacctggaag atcgcaccgg
     1321 cgctcgcggc cggctgcacc gtcgtgcaca agcccgcaga atggtcgcct gtcacggccg
     1381 atctgctggc gaagctcgtc aaggaggccg gcgtccccga cggcgtgctg aacaccgtgc
     1441 atggttttgg cgaggaggcc ggcaaagcgc tgaccgaaca tcccgcgatc aaggcgatcg
     1501 gcttcgtcgg cgagagcacg acgggctcgg ctatcatggc gcagggcgcg ccgaccttga
     1561 aacgcgtgca tttcgaactc ggcggcaaaa acccggtgat cgtgtttgat gacgccgacc
     1621 tcgaccgtgc gctcgatgcc gtcgtcttca tgatctattc gctcaatggc gagcgctgta
     1681 cctcttccag ccggctcttg atccagcaat cgatcgccga aaaattcatg gaaaagctcg
     1741 ccgtgcgcgt gaaagcgctg aaggtcgggc acccgcccga tcccgcaacc gagatcgggc
     1801 cgctgatcca cgaacgacat ctcgcaaagg tctgctctta tttcgacgtg gcgcggaagg
     1861 acggagcggt cattgccgtc ggcggcaaag cgcatgacgg ccccggcggc gggcactatg
     1921 tggagccgac cctggtcatc ggcgcgcaca caaaaatgcg cgtcgcgcag gaggaggtgt
     1981 tcgggccgtt cctcaccgca atcccgttcc gcgacgaggc tgacgcgatc gaaattgcca
     2041 atgatgtgaa ctatggtctc accggctatg tctggaccgg cgatatgggt cgcgcgctgc
     2101 gcgttgccga tgcgctggag gccggcatga tctggctgaa ctccgagaat gtccggcatc
     2161 tgccgacgcc attcggcggc atgaaggcat ccggcatcgg ccgcgacggc ggcgcctatt
     2221 cgttcgactt ctacatggaa accaagcatg tctcgctggc gcggggcacg cacaggattc
     2281 aacgattggg aatttgagac gccgtcgctt ccaggcgacg caaagtctcg aactattgat
     2341 gcgcaattgc gccgcttgag aatctcgaga ttccggatcc gtccttgtgg accgtcccgg
     2401 gacgaccaaa ggaaagccag atgcccgttc cgcaacacgt gttcgatccg ccgttcaaca
     2461 tcatacgctc gagccatgcc gtgctcgacg tgacggatct caagctcagc cgcgagttct
     2521 acgagaccac cgtcggcctg catgtcgagg acagcgacga cgatgcggtg tatttccgcg
     2581 gcagcgagga gcaccagcat cattcgctgg tgctgcgcaa ggcggcggta cccgcctgca
     2641 accggctggg attcaaggtc ggcaacgagc aagacctcga caaggcggcc gcgttctttt
     2701 cgtcaaacgg catcacttac gctttcgccg agccaccgtt ccagggccgt accctgcaat
     2761 ttaccgaccc cttcggtttc cggatcgagc tctacgcgac catggagaag cgcccgcatc
     2821 tgctgcgccg ctatgatctt taccgcggct gccacccgca gcgcatcgat catttcaacg
     2881 tattcgcggc cgaggtacag gacaccgtcg acttctacgc ccggctcggc tttcgcctca
     2941 ccgaatatgc ggaagaggac gggccgaacg gccgcatggc cgcggcctgg ttgcatcgca
     3001 agggcaatgt gcacgatttt gcactgacca acggcaatgg ccctcgcctg catcatttcg
     3061 cctattgggt accgaccgcc atgaacatcc tgcacctttg cgacgtgatg gcctccagcg
     3121 gctatctcaa aaacatcgag cgcgggcctg gccgccacgg catctcgaac gcgttctttc
     3181 tttacatccg cgaccccgat ggccaccggc tcgagctcta caccagcgat tattttacga
     3241 tggaccacga ccatgctccg atgcgctggt cgctgaagga tccgcgccgg caaacgcttt
     3301 ggggcgcgcc ggcgccgcgc tcctggttcg agcagggttc gcccttcacc ggacagaagg
     3361 tgcgcgaacc gctgttcgtc gccgacgtga ccgttgctga ttagagacga taaatgacct
     3421 cccctcgcct tgtgacctac tccatccgcg gatccacgca ttatggcgcc atcgtcgatg
     3481 gcggcgtcgc cgacctctcc gcgcgtttcg gcaaagacta tccgacgctg cgggaagtca
     3541 tcgcggccgg cgcgctgttg aagctcgccg atggcgcggc ggggcgttcg ccggatcatg
     3601 cgctggatgc gattttcttc caaccgccga ttccggcgcc tgaaaagatc atctgcattg
     3661 gcgtcaacta tcccgaccgc aacgccgaat acaaggacgg ctcggacgcc ccgaagtatc
     3721 cgagcatgtt catgcgtacg ccccgctctt tcgtcggcca tgacacgccg ctggtgcgtc
     3781 cgcacgcctc ccagcagctc gactacgagg gcgaactcgc gatcgtcatc ggcaagcccg
     3841 gccgtcacat cggggaaagt caggcgctcg atcacatcgc agcgatcacg ctttgcaacg
     3901 agggcaccat tcgcgactgg gtgcggcacg cgaaattcaa cgtcacgcaa ggcaagaact
     3961 tcgatgcgac cggcagcctt gggccatgga tcgtgcccta tacgagcgag gcccagatcg
     4021 ccgatatccg cctgaccacg cgcgtcaacg gcgagacgag gcaggacgac cgcacctcgc
     4081 ggctgatctt cccgttccgc tacctcatca actacatctc gacctt
//
LOCUS       DATLJT010000289         1885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2773448,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000289 DATLJT010000000
VERSION     DATLJT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773448"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..687)
                     /locus_tag="VM910_06535"
     CDS             complement(121..687)
                     /locus_tag="VM910_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI62234.1"
                     /translation="MSTLRPILTLAAVLALTQTVQAQQGYPVRLELGTTPTPQDLAQL
                     FAIPPDGRGLPPGKGTYAQGEKVYADSCAACHGDKLQGIPTPGIGGDKLIGGRGTLAS
                     ATPVKTVESYWPYATTLFDYVKRSMPFTTPGSLSDEQVYSVVAYILGEANIVPKNAVL
                     DAKSLPDIQMPNRHGFVPDPRPEIQLYR"
     gene            complement(674..1879)
                     /gene="soxC"
                     /locus_tag="VM910_06540"
     CDS             complement(674..1879)
                     /gene="soxC"
                     /locus_tag="VM910_06540"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HVI62235.1"
                     /translation="MPTPARAAERKAPAGAVWRESPNDPTKVPGTPTAEDGGYGSRSQ
                     FATEVRWRYPTPTTLSSWTMTPLDTTEGIITPSGLHFERHHGGIPTIDPARHSLFVHG
                     MVERPKKFTMADVKRFPSVSRLHFIECSGNGLTEWRKPTLKTVQGTHGLLSTSEWTGV
                     QFSTLAREVGLKDGAGWVLAEGADSAVMTRSIPLEKLLTDAIIAYAQNGEALRPEQGY
                     PLRLILPGWEGNTHIKWLRRLDLSDKPFMTREETSKYTDLLGNGKARQFSFTMEAKSV
                     ITFPSGEMKLPGPGVYEITGLAWSGRGRIARVEVSIDGGKNWSLAALQDPVLPICTTR
                     FRFLWFWNGQSSVLQSRATDETGYVQPTLQQLVEIRGLAGPFGSVYHLNAIQSWSVAA
                     DGSVSNVHA"
BASE COUNT          320 a          593 c          611 g          361 t
ORIGIN      
        1 ttcgctgcgt cgtgcgggcg tccgaaagtc gtgtcgctct acccgcctga tccacgaatc
       61 gaccggagca aaccggccga caaatcttct gcgcctacca ttgaaacgct ggcgacgagc
      121 tcagcggtag agttggatct caggccgcgg gtcgggcacg aagccgtggc gattgggcat
      181 ctggatgtcc gggagactct ttgcatcgag cacggcgttc ttcggcacga tattggcttc
      241 gccgagaatg taggcgacca cgctatacac ttgctcatcg ctgagcgacc cgggcgtggt
      301 gaacggcatc gagcgcttga cgtagtcgaa tagcgtagtg gcgtatggcc aataactttc
      361 gaccgtcttg accggggtcg cgcttgcgag cgtgccgcgg ccgccgatta gcttgtcgcc
      421 gccgatgcca ggggtaggga tcccctgcag cttgtcgccg tgacatgccg cacaggaatc
      481 ggcatagact ttctccccct gtgcataagt gccctttccg gggggcaggc cgcgtccgtc
      541 cggcgggatt gcgaagagct gcgccaagtc ctgcggcgtc ggtgtcgttc cgagctcgag
      601 gcgcacagga taaccctgct gcgcctgaac cgtttgcgtg agggcgagga ccgcggccag
      661 cgtcaagatc ggcctaagcg tggacattgc ttacactccc gtcggcggcg accgaccagc
      721 tttggatcgc attgaggtgg tagacggagc cgaatggtcc ggcgagaccg cggatctcca
      781 ccagctgctg caaggtcggc tgcacatagc cggtctcatc cgtggctcgg ctttgcagca
      841 ccgacgactg cccgttccag aaccagagaa accggaatcg cgtcgtgcag atcggcagca
      901 cggggtcttg cagcgcggca aggctccaat tcttgccacc gtcgatcgag acttccaccc
      961 gcgcaatccg cccgcgcccg ctccaggcga gcccggtgat ctcatagacc cccgggccgg
     1021 gcagcttcat ctcgccggaa ggaaaggtga tcaccgactt ggcttccatg gtgaaggaga
     1081 actggcgcgc ttttccgttg ccgagcagat cggtgtattt cgatgtctcc tcgcgcgtca
     1141 tgaacggctt atcgctgagg tctagtcgcc gcagccactt gatgtgggtg ttgccttccc
     1201 aacccggcag aatcaggcgc aggggatacc cttgctcggg ccggagcgct tccccgttct
     1261 gcgcgtaagc gatgatcgcg tccgtcagca gcttttccag cggaatgctg cgcgtcatca
     1321 ccgcggaatc ggcgccttcc gccagcaccc agccagcgcc atctttcagg ccgacctcgc
     1381 gcgccaatgt cgagaattgc accccggtcc attccgaggt gctgagcagg ccgtgggtgc
     1441 cctgaacggt cttcaatgta ggcttgcgcc attcggtgag cccgttgccg gagcactcga
     1501 tgaaatgcag ccgcgagacc gacggaaaac gtttgacgtc cgccatcgtg aacttcttcg
     1561 ggcgctcgac catgccgtgc acgaacaggg aatgcctggc gggatcgatg gtggggatgc
     1621 cgccgtgatg tctttcaaag tgcagcccag agggagtgat gatgccctcg gtcgtatcga
     1681 gcggcgtcat cgtccacgac gagagcgtcg tcggggttgg gtagcgccag cgcacctcgg
     1741 tcgcaaattg cgagcgcgag ccgtatccgc cgtcttcggc ggtcggagtg ccaggcacct
     1801 tggtcggatc gttcggcgac tctcgccaga cggcgcccgc tggcgccttg cgctctgccg
     1861 cgcgcgccgg cgtcggcaag ggggc
//
LOCUS       DATLJT010000290         3854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3002839,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000290 DATLJT010000000
VERSION     DATLJT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3002839"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..589
                     /locus_tag="VM910_06545"
     CDS             191..589
                     /locus_tag="VM910_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI62236.1"
                     /translation="MSQQVARTPGAKRAGDGEYVFDLAAVNHILGGPDYSTANGACVE
                     GDRMIVALMRMPAGTGAEPHSHPNEQWIYILQGTFRARIGEAEIEAKPGSVVYVPSNV
                     VHSGGATLEEDVVFFTCKDASHSLHGVKAA"
     gene            797..1783
                     /locus_tag="VM910_06550"
     CDS             797..1783
                     /locus_tag="VM910_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62237.1"
                     /translation="MRWIGLGLALLCLAFGGVTASAQDKYPSRPVKVVVPYAPGGATD
                     ITARLFGEQMRQSLGQQFVVESKPGAFGILAIEEMARSKPDGYTLMVGNVTTNAITPV
                     LFAKKFSINFEKDVVSVSRLAIYPSFLLTTTQNFEPKTVAELIAYAKKNPGKVRYTSA
                     GVGSFPHYDVEVFSKRAGIEMLHIPNKTGAAGMINDLVVGDAQVAFINAASTASMIKA
                     GKLRPIAVLAEARLAEYPDVPTLAEAGYPGVGTLHWQSMLAPANTPKEVLATLFKAIV
                     EAADAPQLQEAFKKQLVSVKPNASLQEAQSWLDSELAAWRKTVAEVKIELAD"
     gene            1871..2851
                     /locus_tag="VM910_06555"
     CDS             1871..2851
                     /locus_tag="VM910_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62238.1"
                     /translation="MKRLSILLVFLFALGGVEVAAQDKYPSKPVKVVVPFGPGSATDI
                     VMRIVGEHMRPILGQPVVIENKPGAFGILAIEEMARSRPDGYTLQIGNPGTNVLAPII
                     YKKKFKIDYEKDVTLVTRLSEVPLVLGATTKDFPPKTYAEFIAYAKANPGKVRYASVG
                     IGSNNHYDMEAFARWADIVLAHLPNKGGGAAITNDLVTGDAHVALLNAASSMGVVKAG
                     QVRALAVMSDQRVPEYADVPTLTELGYANAKGLWSALYAPAATPRDVLEIVRKAAVQA
                     LNSEQVATAFKQQMIRPLPSASIEEAQAWNKAEVAYWKKVTDEVKVELPD"
     gene            2892..>3854
                     /locus_tag="VM910_06560"
     CDS             2892..>3854
                     /locus_tag="VM910_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVI62239.1"
                     /translation="MKMEPAPGVPVYGIVVEKDVDVPMRDGARLKADVFRPDDSGTFP
                     AILNLGPYQKDKLWIPPGNLEEKPNPLMNWETVNPQWWVPNGYAAVRVDGRGSGKSPG
                     ACEPWSLAESIDLYDAIEWAAAQPWCSGKVGLSGISYFAINQWFVANHQPPSLAAIIP
                     WEGFADLYRDALFHGGLLSLFMPNWYMAHLMHHVAGRASRHHPNRWQTNTLHFWLHNN
                     LDSGAFRGAQAQWDKIKVPMFSVGNWSGMGLHLRGNTEAFMRAATPHKKLRIHAGTHV
                     HPFYSEDGRRDQLRFFDYWLKGIDNGVMAEPPVKLAIRKGRDQVE"
BASE COUNT          738 a         1184 c         1256 g          676 t
ORIGIN      
        1 cttcgacatg ggcatgccgg agggcgtggc gcaggttcgc ggcctgccga tttcggccgc
       61 cgagcagtcg gccattctcg gcgggcgggc gcgggcgttg ctcggcgcgg ccggcgacaa
      121 gacgcgggcg tttgcctgac gaaactcgct ctgtggatga gagtgcagcg ggaagggaag
      181 gagacgaaaa atgtcgcaac aagtggcgcg gaccccgggt gccaagcgcg cgggcgacgg
      241 tgaatacgtg ttcgatctcg ccgcggtaaa ccacatcctc ggcggccccg actattcgac
      301 cgccaacggc gcctgcgtcg agggtgaccg catgatcgtc gcgctgatgc ggatgccggc
      361 cggcaccggc gcagagccgc attcgcaccc gaacgagcaa tggatctaca ttctgcaagg
      421 cacgtttcgg gcaaggatcg gcgaagcgga gatcgaggcc aagcccggct cggtagtcta
      481 cgttccctcc aacgtcgtgc actccggcgg ggcgacgctc gaggaagacg tcgtcttctt
      541 cacatgcaag gatgcgtccc acagcctgca cggcgtcaag gcggcgtgac accaagtttc
      601 gtggcagccc ctttgatggt gcttcgcgtc cggtgacgga gttgaagctc gcgaagcctg
      661 gtagcgtctg cgagtcgagg ggcgacaaca tcgcgcggtt gattgcctgt tttcgtcccc
      721 cgcgcctggg ctacaatcac tggcaagctg atacccgtcg gggtggcgat ttcagcgggt
      781 tcaccgggag gacgtgatga gatggatcgg tttgggtctg gcgctgctgt gtcttgcgtt
      841 cggcggcgtc acggcctcgg cgcaggacaa atatccttcg cggccggtga aggtcgtcgt
      901 accgtatgct cccggcggcg ccaccgacat caccgcgcgc ctgttcggcg agcagatgcg
      961 ccaaagcctc ggccagcagt tcgtggtcga gagcaagccg ggcgcgtttg gcattctcgc
     1021 catcgaagag atggctcgct ccaagccgga cggctacacg ttgatggtgg gcaacgtcac
     1081 gaccaatgcc atcacaccgg tcctgttcgc gaagaagttc tccatcaact tcgagaagga
     1141 cgtggtgtcg gtgtcgcgcc tcgccatcta tccgtccttc ctcctcacca ccacgcagaa
     1201 tttcgagccg aagacggtgg cggagttgat cgcgtatgcg aagaagaatc ccggcaaggt
     1261 tcgctacacc agcgccggcg tcggcagctt cccccattac gacgtcgagg tcttttccaa
     1321 gcgcgccggc atcgagatgc tgcacatccc caacaagacc ggcgccgccg gcatgatcaa
     1381 cgatctcgtc gttggcgacg cacaggtcgc gttcatcaat gccgcgagca cggcgtcgat
     1441 gatcaaggcc ggcaagctgc ggccgatcgc ggtgctggcg gaggcgcggc tggcggaata
     1501 tcccgatgtc ccgacgctag ccgaggccgg ctatcccggg gtgggcactt tgcactggca
     1561 gagcatgttg gcaccggcca atacgccgaa agaggttctg gccaccctct tcaaggcgat
     1621 cgtcgaggcc gcggatgccc cgcagctgca ggaggccttc aagaagcagc ttgtcagcgt
     1681 caagccgaac gcatcattgc aggaggcgca atcgtggctc gacagcgagc ttgccgcatg
     1741 gcgaaagacc gtcgccgagg tcaagatcga attggccgac tagcgtgtgg cgcgacgtcg
     1801 gcgtaagcgc gaactgcttg cgcatgaact ttcggatgtc gcgggagcac cgtctgaagc
     1861 gaaaaatgtc atgaagcgct tgagcattct gttggtgttt ctcttcgccc tcggcggcgt
     1921 cgaggtcgcc gcgcaagaca aatatccttc gaagccggtc aaagtcgtcg tgccgttcgg
     1981 accgggcagc gccaccgata tcgtcatgcg cattgtcggc gagcatatgc ggccgattct
     2041 cgggcagccg gtggtgatcg aaaacaagcc cggcgccttc ggcatccttg cgatcgagga
     2101 gatggcgcgt tcgagaccgg acggctatac gctgcaaatc ggcaatcccg gtaccaatgt
     2161 actcgcgccc atcatctaca agaagaaatt caagatcgac tacgagaagg acgtcacctt
     2221 ggtgacgagg ctaagcgagg ttccgctcgt gctcggcgcc accaccaagg acttcccgcc
     2281 caagacctat gccgagttca tcgcctatgc caaggccaac cccggcaagg tgcgctatgc
     2341 cagtgtcggc atcggcagca acaatcacta cgacatggaa gccttcgctc gatgggccga
     2401 tatcgtgctc gcccatctgc cgaacaaggg cggcggtgcc gccatcacca acgatctcgt
     2461 caccggcgac gcccatgtcg cgctgctcaa cgccgcaagc tcgatgggcg tggtcaaggc
     2521 cgggcaggtt cgagccttgg cggtgatgtc ggaccagcgc gtgcccgaat acgccgatgt
     2581 gccaaccctc accgagctcg gctatgccaa tgccaagggc ttgtggtcgg cgttatatgc
     2641 gcccgccgcc acgccgcgcg acgtgctcga gatcgtgcgc aaggcggccg tgcaggcgct
     2701 caattccgag caggtggcga ccgccttcaa gcagcagatg atcaggcctc ttcccagcgc
     2761 gtcgatcgaa gaagcgcaag catggaacaa ggccgaggtg gcgtactgga agaaggtcac
     2821 cgatgaggtg aaggtcgagc tgccggattg agcggcggca agagcgggcg tgcagcgggg
     2881 agagaacagc aatgaagatg gagcctgctc ccggcgtgcc ggtctacggc atcgtcgtcg
     2941 aaaaagacgt cgacgtcccg atgcgggacg gtgcgcgcct caaagccgat gtgttccggc
     3001 cggatgattc cggcaccttc cccgccatct tgaatttggg tccttatcag aaggacaagc
     3061 tgtggattcc gccgggcaac ctggaggaaa agccgaaccc gctgatgaac tgggagacgg
     3121 tcaacccgca atggtgggtg cccaacggct acgccgcggt gcgcgtcgac ggccgcggca
     3181 gcgggaaatc ccccggcgcg tgcgagccgt ggtcgctcgc ggaatcgatc gatctctacg
     3241 acgcgatcga gtgggcggcg gcgcagccct ggtgctcggg caaggtcggg ctgtccggca
     3301 tatcctattt cgccatcaac cagtggttcg tcgccaatca tcagccgccg tcattggcgg
     3361 cgatcattcc ttgggaaggt ttcgccgatc tctaccgcga cgccctgttc cacggcggac
     3421 tgctcagcct gttcatgccg aattggtata tggcgcactt gatgcaccac gtcgccggtc
     3481 gggcgtcccg gcaccatccg aacaggtggc agaccaatac gctgcatttc tggttgcaca
     3541 acaatctcga cagcggcgcc ttccgcggcg cgcaggcgca atgggacaag atcaaggtgc
     3601 cgatgttcag cgtcggcaat tggtcgggca tgggattgca tctacgcggg aacaccgagg
     3661 ccttcatgcg ggcggcgacg ccgcacaaga agcttcgcat ccacgccggc acgcatgtgc
     3721 atccgttcta ctccgaggac ggacggcgcg atcagctgcg tttcttcgac tactggctca
     3781 aaggcatcga caacggcgtg atggcggagc cgccggtgaa gctcgcgatc cgtaagggcc
     3841 gcgaccaagt ggag
//
LOCUS       DATLJT010000291         8320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_475799,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000291 DATLJT010000000
VERSION     DATLJT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475799"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..559
                     /locus_tag="VM910_06565"
     CDS             74..559
                     /locus_tag="VM910_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62240.1"
                     /translation="MLFNPASATYAEYWLKPFQAAADAFAVEAIAAPVRDGSELDSVF
                     AALAREPNSGLIAMPDSFTDANRVEITSLAARYRLPAVYPYRHFAEVGGLLSYGVDQN
                     DNFRRAAGYVDRILKGEKPSELPVQAPVKFELVINLKAAKALGLEVPAQLLGRADEVI
                     E"
     gene            668..1111
                     /locus_tag="VM910_06570"
     CDS             668..1111
                     /locus_tag="VM910_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62241.1"
                     /translation="MAGDPGLWDVAWNWPLPFVTAVAVLAVPMAGIIWFICQLKYKRQ
                     IATLKDRVELADERLGDAKEKIVKAEEAKTALHTTVKELVAQIEKLKKRKAQLPRELQ
                     SIVDRLATSSATASNQVSKLSEAQEAVAAAVSKHRDESHEGGAGH"
     gene            1086..1187
                     /locus_tag="VM910_06575"
                     /pseudo
     CDS             1086..1187
                     /locus_tag="VM910_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496200.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1211..1453
                     /locus_tag="VM910_06580"
     CDS             1211..1453
                     /locus_tag="VM910_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62242.1"
                     /translation="MKLPRRQFLHLAAGAAALPTVSRVAGAQAYPAQPVRVVVPFAPA
                     LADLLAGQVHVMFPLQRRDYDGQSRPLLIRWQRQRA"
     gene            complement(2399..2776)
                     /locus_tag="VM910_06585"
     CDS             complement(2399..2776)
                     /locus_tag="VM910_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62243.1"
                     /translation="MMKVKRKGSMAVRTKKGRRTAGHDAVATIRLSSELRESVDAWAA
                     KQSDKPARPEAILRLVELGLERTHRRESAPNRAAKASEMAAQEIDRITDPSATEEERQ
                     LRKRRLIKGPKEFRDIRKKSRED"
     gene            complement(3418..3564)
                     /locus_tag="VM910_06590"
     CDS             complement(3418..3564)
                     /locus_tag="VM910_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI62244.1"
                     /translation="MSATAIAAQLSARKIATARGGAWSHVQVGTILRRAMAERAGSYP
                     QAYE"
     gene            complement(3798..5903)
                     /locus_tag="VM910_06595"
     CDS             complement(3798..5903)
                     /locus_tag="VM910_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI62245.1"
                     /translation="MRAKLPDKNGFVERDGVNLYYEVYGDGPDTMLFIPPWSIVHSRI
                     YKAQLPYLSERFRCVAYDGRGNGKSDRPPDVAAYSLDEYVADALAVMDATEAGQAILV
                     GLSFGGFLACVLAAHYPKRVRAAVLAGTVASVGANYPYLSPKHFLAKHENWEGWNKYN
                     REHWLADYPDFADHFVCNIFSEPHSTKHIEDGMAWANETTGPVLIKTVEARAIPPQFE
                     VSEVMYRKIRCPMLVIHGDNDQIQPYERGRLVAELTGAELVTIPGGGHHPLGRFPAKC
                     NALITDFLHCKLGVPASRRRASPMRRGRRALYLSSPIGLGHARRDLAIVRELRNLHPD
                     LQVDWLAQDPVTRLLEANRECVHPLSGRLASETRHIELESGEHELHCFQAIRRMDEVL
                     IHNFMIFQDAVEECAYDLVIADEAWDIDHYWHEHPELKKAKLAWFTDFVGYVPIPSAG
                     AHEAFLTTDYNAEMIEHIERHPHVRDRAIFVGNPDDIIPLSFGKDLPQMRDWVPKHFE
                     FAGYIIGEHPRTFGTRAELRQRLGYQPGERVCIVTVGGSGVGSHLIKRILQSYPLARA
                     RVPELRMIVVAGPRIDPRALQAPAGVEVRAFVPNLDRHLAGCDLALVQGGLTTCMELT
                     AAGTPFLYFPLKNHFEQNFHVAHRLDRYRAGRRMEFATSTPYMIADAMVAVLNARPKY
                     RPVEADGAARAARMLADLI"
     gene            complement(5908..6522)
                     /locus_tag="VM910_06600"
     CDS             complement(5908..6522)
                     /locus_tag="VM910_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVI62246.1"
                     /translation="MVRVAIKQAETSTALLEAAKKVLRQNGYSGLSTRDVAATAGVPL
                     SQIHYHFGSKQGMMLAVFEYLNAQLLERQQSMFSDPSLKISEQWDLACQSLDDDIGSG
                     YVRVLQELIAASWVDAEVAKVIRAGIMGWIDLIVQVARRAERELGGLGPFSPEELGAI
                     VADAFIGAESLYLLGIEKRGSPVRQALRRFGDLIRIAESASSKR"
     gene            complement(6693..7757)
                     /locus_tag="VM910_06605"
     CDS             complement(6693..7757)
                     /locus_tag="VM910_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI62247.1"
                     /translation="MSHIDNDKLGALVGKMLGDLGGAFSVPTVRIGFRMGLFEALHRH
                     GPARISELAARTGLLDRYVHEWAFAQAANGYIAFDAEQGRFSLTPEQAMIFAAKDSPV
                     YLEGAFDLAAAMVEGQHKVEAAFRTGDGVAWGDSSGCLFCAVGAFFRPGYVNSIVQSW
                     LPSLEGVQQKLQNGAMVADIGCGVGFSTLLMANAFPASSFVGYDFHAPSIEQANAHAK
                     AHGFADRVRFVTGRAKDIAERDFDLVTMFDCLHDMGDPRGCAKHVRKLLKPGGTWMIV
                     EPIAANDPAENVGNPVSRLYYNASTMICVPTSLAQEVGEALGAQAGEAKLTDVLKEGG
                     FRHVRRATQGPFNMVLEARP"
     gene            8215..>8320
                     /locus_tag="VM910_06610"
     CDS             8215..>8320
                     /locus_tag="VM910_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HVI62248.1"
                     /translation="MRYELTDDEWTAIKPMLPNKPRGVPRVNDRRVLNG"
BASE COUNT         1615 a         2503 c         2519 g         1683 t
ORIGIN      
        1 tttcggaacc gacgcaggct ggcaagtggg tggaactgct caaggaggtt gagcgcgcat
       61 tgctagggtc gcgatattgt tcaacccagc atcggcgacg tatgccgaat attggctgaa
      121 gcccttccaa gcggccgctg atgccttcgc agtggaggcg attgcagcac ccgttcgcga
      181 cgggtctgaa ctggattccg tctttgctgc actggcacgc gagccgaata gtggccttat
      241 cgcgatgccg gatagcttca cggatgccaa tcgcgtagag attacctcgc tagcggctcg
      301 ctatcgtctc cctgctgtct atccgtaccg tcacttcgct gaagtcggtg gcctgttgtc
      361 ctacggcgtt gaccagaacg ataattttcg gcgcgcggcg ggttatgtcg atcgcatcct
      421 caagggagag aagccgagcg agcttcctgt ccaggcccca gtcaagttcg agctggtgat
      481 caacctgaag gccgccaagg cgctcgggct cgaagtgcca gcacaactgc tcggccgcgc
      541 cgatgaggtg atcgaatgag cgccgctgct tcataatgct gctcggcggc gcggcggcgt
      601 ggccgcgcgc ggcgcgcgcg cagcagccgg cgctccgcgt gaccgaggag gaaatggggc
      661 gatagtcatg gccggtgacc ctggcttgtg ggacgtggcg tggaattggc cccttccgtt
      721 tgtgacggcc gtggcggtgc tcgccgtgcc gatggccggg ataatatggt tcatttgcca
      781 acttaaatac aaacggcaga ttgcaacgtt gaaagatcgc gttgaactcg ccgacgaaag
      841 attaggtgat gctaaagaga aaatcgtaaa ggcggaggaa gcgaagaccg ccctccacac
      901 gacggtaaag gagttggttg cacagatcga aaagcttaaa aaacgcaagg cacaattgcc
      961 acgcgagttg cagtctatcg tcgatcgtct agctacgtcg tccgcaacgg cctcaaatca
     1021 ggtaagcaag ctcagcgagg ctcaagaagc tgtggctgcc gctgtgtcaa aacacaggga
     1081 tgagagtcac gaaggtggag ctggtcatta atctcaaggc tgccaaggcg ctcggcgtca
     1141 ctttcccgct cacgctgctc gcgcgcgccg acgaagtgat cgaatgatgt gaagcgagag
     1201 ggaggttcaa atgaaacttc cccgccgaca atttctgcat ctggccgcgg gcgcggccgc
     1261 gctgccgacc gtatcgcggg tcgcgggggc gcaagcctat ccggcgcagc cggtgcgcgt
     1321 ggtcgtcccc tttgcaccgg cgctcgccga cctgctcgcc gggcaggtgc acgtcatgtt
     1381 cccgcttcaa cgaagggact atgacggcca gtctcgccca ctcttgatca ggtggcaacg
     1441 ccagcgggct tagcaggaca agcggccccc cgcttcacga aagtgcaagg ctagtttgct
     1501 acattgatag cggagtcgcg cttgccccaa aacccggaac agcatgcggt ttggtcggtc
     1561 gcagtttgct ggcctcaatc gatgggtcgt ccgcatagcg cattaaggta atcgttcatc
     1621 gcgctttgaa cagaactgcc tcgcaattct ggacgcgccc tgacgtactc tcgaagaaag
     1681 ctcgcaagct gactagctgt cagttggctt ctcacaatgc actggccgta atgccgtgct
     1741 gccctctggg cgggctcagg tccggctatg gttcctagca tctcaatgga cctagctata
     1801 tacgcaacgc gatcatcctc gtgcaatctt tcccactggg agaacgtgta atcgaggtgg
     1861 acttccgcag tagcgggcca cgacaacagc aacaagaccg ccgcatattt gaacatagca
     1921 gcctcgaccc catctcgatc aatcgttttg ccgccatacg ccgcctaaaa ggtcaagtct
     1981 gttgtggaag atcaagccgg ccgctatgac gaagaaggtc gagcacgccc ttcgcttgat
     2041 gtgtgcttgg cgccaagcag cacacaaggg taaagaatat gcgaattgcg aggagtccag
     2101 tcgagccgcc ggctcgagcg tgaggccggc cgcaggctcg cggatgtcgg ggccgaaacc
     2161 gtccaggtcc ccggcggaca tggccgcaac ccggcgggga ctcttcgtgc ccgacgctgc
     2221 gtgaggaacc tctagcgcac ccgtgcgttt gccgcagcgg agcccttgta acatagccct
     2281 ccgtacacca caccaaatga gggttcccga gaccccgccc gctacctcgg actcgggcgg
     2341 ggttctcgtt tgtggcgaat gacttgaacc ccactggctg aatatcggac catcgctgtc
     2401 aatcttcacg cgatttcttg cggatgtctc tgaactcttt cggtcctttg ataagtctcc
     2461 gcttgcgaag ttggcgttct tcctcggttg ctgacggatc agtgatacgg tctatctcct
     2521 gggcggccat ttcggatgct ttcgcggctc tgttcggagc cgactccctt cgatgagtcc
     2581 tttcaagccc cagctcgaca agccgaagga tggcttcggg ccgtgccggt ttgtcgcttt
     2641 gtttagctgc ccaggcgtcc acactttcac gcagttcgga cgaaagacgg atggtcgcta
     2701 cggcatcatg cccggcggtg cgacggcctt tctttgtacg cacggccatg cttccttttc
     2761 ttttcacctt catcatctac cgacccagcg gcgatcttca atccccgcga acgaaggcgg
     2821 cggtgatccc cggcgtttcc aggagcgtgt gccctatggg ctgttcgtgc ccgatgtgcg
     2881 cgactgcgta cacaccctcg acggcagtga gtcagtttga ttttagtgaa tggcccgagg
     2941 ctcgggcttc agcgcgctcg gcaagaactt cataatcgtg ggcaatgcgt agcatcgcat
     3001 tttttgattg aggttcggct atttgatccg cgcgactacg agcttcttct gcgcgctcac
     3061 gccagtgcct ggggtcgtta aggaaggacc taggttccat tgtctagccc cgctacaagg
     3121 tggggtactc cagccacaaa ataatgtcac ataatcgacc ggcagcattg tgatgggcgt
     3181 cacattttgc ggcaatttcg catcccgctt agttccattt cactggcgcg gcatgtgcta
     3241 tgcgcatcca ttcagcctgg atgtcgcttc acgttcgcca agctggcgat cgccaaccta
     3301 ccgaaggaca ttttggtcag tgttgaccag cgcattcccg gtggcctcga tcttgaggaa
     3361 tgggcgctgc tgcggcgtgt gcttgggcta atcaagaaat ccgcgcctgc ggggtcctca
     3421 ttcgtaagct tgggggtagc ttccggcacg ctcggccatt gcccgcctga gtatggtgcc
     3481 aacttgaaca tgcgaccagg ccccgccgcg agccgtcgcg atcttgcggg cgctgagctg
     3541 ggcagcaatg gcggtggcgc tcacatgtcc cgccgcttgt atgtcgcgaa tgacgggcaa
     3601 tgagcgacat cgacactggg gtggtggaca ggagacatcc ccaccaattc acgtccatcg
     3661 gctggatcac cgcggcggca tcgttgcgcc ggtgctgcat gatccaggaa cgagcacaag
     3721 cgtcatgccg ctcccgcact cgtcgtattc gatgctcccg cgcgcgtcgt cgatcatgat
     3781 ctgatcgtca gtcgagatca gatcaggtcc gcgagcatcc gggcagcacg cgccgcgcca
     3841 tcggcttcga ccggtctgta tttcggtcgc gcgttcagga ccgcgaccat cgcatcggcg
     3901 atcatgtaag gggtcgaggt cgcgaactcc atccgccgcc ccgcgcggta gcggtcgagc
     3961 cggtgcgcga cgtggaagtt ctgttcgaag tgattcttga gcggaaagta aaggaacggt
     4021 gttcccgcgg cggtcagctc catgcaggtg gtgagaccac cttggacgag ggcgagatcg
     4081 cagcctgcca gatgccggtc gagattgggc acgaacgcgc gcacttcgac cccggcgggc
     4141 gcttgcagcg cgcgcgggtc gatgcgtggc cctgccacga caatcatgcg tagctcaggt
     4201 actcgggcgc gcgcgagggg gtagctttgc aggatgcgct tgatgagatg cgacccgacg
     4261 ccggaacctc cgacggtcac gatgcacacg cgctcgcccg gctgatagcc gagccgctga
     4321 cgcaactccg cccgtgtgcc gaacgtccgc gggtgctcgc caatgatgta gccggcaaac
     4381 tcaaagtgct tgggcaccca gtcacgcatc tgtggcaagt cctttccaaa gctcagcgga
     4441 atgatgtcgt ctggattgcc gacgaagatg gcgcggtcgc gcacatgcgg gtgccgctcg
     4501 atatgctcga tcatctcggc attgtagtcc gtcgtgagaa aggcttcgtg cgcaccagct
     4561 gaagggatgg gtacgtagcc cacgaaatcc gtgaaccagg cgagctttgc tttcttgagc
     4621 tccggatgtt cgtgccagta atggtcaatg tcccaggctt catcagcgat caccagatcg
     4681 taggcgcatt cctcaacggc atcctggaag atcatgaaat tatggatcag cacctcatcc
     4741 atgcggcgaa tggcttggaa acagtgcagc tcgtgctccc ccgattcgag ctcgatgtgg
     4801 cgcgtttcgc tggcgagccg cccgctcagg ggatgcacgc actcccggtt cgcctcgagc
     4861 aggcgggtga ctggatcctg ggcaagccag tcaacctgca gatccgggtg gaggtttcgg
     4921 agttcccgca caatagcgag atcgcggcgc gcatgtccga gaccgattgg ggacgagaga
     4981 taaagcgctc gcctgccacg gcgcatcggg cttgcacgcc gtcgcgaagc cggaacgccc
     5041 agcttgcagt gcagaaaatc ggtgatcagc gcattgcact tggccggaaa gcgaccgagc
     5101 gggtggtgcc cgccgcccgg aatcgtgacg agctccgcgc cggtcagctc ggctacaagc
     5161 ctcccacgct cgtagggctg gatttggtcg ttgtcgccgt ggatcacgag catcgggcag
     5221 cggatcttgc ggtacatcac ttcactgact tcgaactgcg gcgggattgc acgtgcttcc
     5281 acggtcttta tcaggacggg gccggtagtc tcgttggccc acgccatgcc gtcttcgatg
     5341 tgtttggtcg agtggggctc cgagaagatg ttgcagacga agtgatcggc gaagtccgga
     5401 tagtccgcga gccagtgttc gcgattgtac ttgttccatc cctcccagtt ctcgtgcttg
     5461 gcaaggaaat gtttcgggct caggtacgga tagttggcgc cgacgctcgc caccgtgccc
     5521 gccaggacgg cggccctaac gcgcttggga tagtgggcgg cgaggacgca agcgaggaag
     5581 ccaccgaagg agaggccgac gaggattgcc tggcccgctt cggtggcatc catgacggcc
     5641 agtgcgtcgg cgacgtactc atccagcgag taggccgcca cgtcgggagg ccgatcggac
     5701 ttgccgttcc cgcggccgtc atacgcaacg cagcgaaaac gctcgctgag atagggcagc
     5761 tgcgccttgt agatgcgcga gtgcacgatg ctccacggcg gtatgaacag catcgtgtcc
     5821 ggaccatcgc catagacctc gtaatagaga ttcacgccgt cgcgctcgac gaatccattc
     5881 ttatcaggca gcttcgcgcg catgacatca tctcttcgag gaagcgcttt ccgcaatgcg
     5941 aatgagatcg ccgaaccgcc gcagcgcctg acgtactggt gaccctctct tctcaatgcc
     6001 gagaaggtaa aggctttccg caccgataaa tgcatcggcc acgatcgccc cgagttcctc
     6061 gggtgagaag ggtcctaggc cgccaagctc gcgctcagct cggcgcgcca cctggacgat
     6121 gagatcgatc cagcccatga tcccggctct gatgaccttg gcgacctcgg cgtccaccca
     6181 gctcgccgcg atcagttcct gcaaaacgcg gacgtaaccg gagcctatgt cgtcgtcgag
     6241 ggactgacac gccaggtccc attgttccga aatcttgagg ctcgggtcgc tgaacatgga
     6301 ttgctggcgc tcgaggagct gggcgttgag atattcgaac accgccagca tcatgccctg
     6361 cttggacccg aagtgataat ggatctggct caacggaacg ccggcggtgg cggcgacgtc
     6421 gcgcgtggaa agcccggaat agccgttttg ccggagcacc ttcttagctg cttcgagcag
     6481 ggccgtgctg gtctctgctt gctttatcgc gacgcgcacc atgggctgtc ccgacctctc
     6541 tcgttggcgt aaacgatcgc ttacgcatcc gctgcccgga tgggcccatc agtagcgtct
     6601 cgagctattg ctcggaactg tagccacaga cgtgacgagg tggtccgggc gctctggatg
     6661 gtacacgcgc ttgcccgact gaagctgtct cctcaaggcc gagcttcgag caccatattg
     6721 aagggacctt gggttgcgcg ccgcacatgg cggaagccgc cttcttttag gacgtcggtc
     6781 aatttggcct ctcccgcctg agcgccgaga gcctctccaa cttcctgcgc gagcgacgtg
     6841 ggcacgcaga tcattgtcga ggcgttgtag tagagccggc tgacaggatt gccgacattc
     6901 tctgcaggat cattagccgc aatgggctct acgatcatcc aagtgccgcc aggtttcaac
     6961 agcttacgca cgtgcttggc gcagccacgc ggatcgccca tatcgtgcag gcaatcgaac
     7021 atggtgacga gatcgaagtc gcgttcagcg atgtccttgg cacgaccggt cacgaagcgc
     7081 acgcggtccg cgaagccatg cgctttggca tgcgcattcg cctgctcgat cgatggcgcg
     7141 tgaaagtcgt agccgacaaa tgagctcgcc ggaaaagcgt tcgccatcaa aagcgtcgag
     7201 aaccccacac cgcagccgat gtcggccacc atggctccgt tctgtaactt ttgctggaca
     7261 ccctccaacg agggtagcca ggactggacg atgctgttga cgtagcccgg ccgaaagaag
     7321 gcaccgacgg cgcagaacag gcacccactg ctgtcgcccc aagcaactcc gtcgccggtc
     7381 cggaacgccg cttcgacttt gtgctgtcct tccaccatgg ctgccgcgag atcgaaagca
     7441 ccttccagat acacgggact gtccttggcc gcgaagatca ttgcctgctc tggcgtcaag
     7501 ctgaaccgtc cttgctccgc atcgaaggcg atataaccgt tggcagcctg agcgaatgcc
     7561 cattcatgta catagcggtc gagtagtccg gtccgcgcgg cgagttcgga aatccgcgct
     7621 gggccgtgac gatgaagggc ttcgaacagg cccatccgga acccgatgcg gaccgtgggt
     7681 acgctaaacg cgccgcccaa atccccaagc atctttccga ccaatgctcc aagcttgtcg
     7741 ttgtcgatgt gactcatcac tgctggattt ctcctgaaga ggtggcacag ggaaggtgac
     7801 tccaagaacc gacgatcccg cgtcgctttt tacagcgctc aggatctccg tgcgcgtaag
     7861 gccgatatcc tttagggtac gatcgctcag cgaccagagc tgagccgttg cccactgctc
     7921 gaggcgccaa gtcgtgtatc ccaccaacca gcgctccaac ctgaccgcca aagattgcct
     7981 agccatgggt agagccgcgg ccagcgctga agatcgggtt catgactatc cctgtcggac
     8041 ttgcgtataa ttcggtcgat cgaccgaata tatttgacgg gataattcgg tcgatcgacc
     8101 gagtcaaggg gtgtcattca catgctcgtg aagtcgcact gcacctgcga cagggcctta
     8161 ttgagactga ccgccgggac gtgattcaag ctcccaaact tggagcctcg aatcatgcgc
     8221 tacgaactca ccgacgatga gtggactgcc atcaagccga tgctgcccaa caagccgcgc
     8281 ggcgtgcctc gggtgaacga tcgccgtgtc ctcaacggca
//
LOCUS       DATLJT010000292         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1165363,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000292 DATLJT010000000
VERSION     DATLJT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165363"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VM910_06615"
     CDS             <1..360
                     /locus_tag="VM910_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HVI62249.1"
                     /translation="LNMRLRDDYTQDCVKGMLRWNKVISTAGYDYRLTLPHVAFHRDI
                     GEFKDVHATPDGILIDDATWAKRRDEWLPSTSDGDFITSLMTPVTEIGEFAPWISPPK
                     VGIDNKPGDFEYVKIES"
     gene            complement(484..1284)
                     /locus_tag="VM910_06620"
     CDS             complement(484..1284)
                     /locus_tag="VM910_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62250.1"
                     /translation="MFKDLFSLEGRTALVTGGSRGIGKMIAAGFLAQGAAKVYITARK
                     AGPCEATAKELTATYGGACIALPIDISTVAGCEKLAGEIIKLQPKLDILVNNAGAAWG
                     ADFDEFPENGWDKVMDLNVKALFFLTKALAKPLRAAASAERPAKVINIASIDGIFVNP
                     NETYSYAASKAAVIHLTRRMATKLIRDHVNVTAIAPGAFKSDMNRAARDQADEVAKRV
                     PAGRVGTDEDMAGVAIYLASRAGDYVVGTTIAVDGGVVYASAGLEIAG"
     gene            complement(1417..2472)
                     /locus_tag="VM910_06625"
     CDS             complement(1417..2472)
                     /locus_tag="VM910_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI62251.1"
                     /translation="MKQFRNTVIRAGLGALYFSGAHVLLRPIFSGVGAIFMLHHVRPG
                     RTGDFQPNRHLEVTPDFLRAMLSHLRTQAIDIISMDEVHKRLLERNFSRRFACFTLDD
                     GYRDNRDFALPVLREFEAPFTLFVASDFAEGTGHLWWIALEMAIARASAIELSMGGTP
                     TRLDTAGAAAKQAAFERLHDWLRTLPEPDMQREVHALCMRHGIDDAAICRDLCMSWEE
                     LKSFAADPLVTVGAHSVSHCNLAHQSEGKARQELNSSRTRLERALQRPVLHLAYPYGD
                     RLAAGAREFALARAAGYKTAVTTRPGMIFPESGQHLTALPRVSLNGNYQDARILPVLT
                     SGAATAMWNGFRRVDAA"
     gene            2746..>3515
                     /locus_tag="VM910_06630"
     CDS             2746..>3515
                     /locus_tag="VM910_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI62252.1"
                     /translation="MHGPFRWSSKWWPGVIPLVVLWAFAAWTNTDPVEADLAARSTAA
                     LKDVVLDKTRIAVEGRDVTLGADAFSEDGRRSALASVRAVPGVRLVNDETRLVPEAKP
                     FVWSAERDVVRVTLGGSAPLPAVRAKLADAARAGLGGVEVADQMSLARGAPPRFETAA
                     LLLIDQIGKLKDGKITISDNAVTLSGMARELGGREAIAASLKDLPEGFSVKANDVKAP
                     PYVFQAYKDPVAVTLTLSGNVPDNNIHAAIAQAASRKF"
BASE COUNT          628 a         1094 c         1146 g          647 t
ORIGIN      
        1 ctcaacatgc gccttcgcga cgactacacg caggattgcg tcaagggcat gctgcgctgg
       61 aacaaggtga tctcgaccgc gggctacgac tacaggctga cgctgccgca tgtcgccttc
      121 catcgcgaca tcggcgagtt caaggacgtg cacgcgacgc cggacggcat cctgatcgac
      181 gatgcgacct gggcgaagcg tcgcgacgag tggctgccgt cgaccagtga cggcgatttc
      241 atcacttcgc tcatgacgcc ggtgaccgag atcggcgagt tcgcgccctg gatctcgccg
      301 cccaaggtcg gcatcgacaa caagcccggc gacttcgaat atgtaaaaat cgagtcgtga
      361 gaagccggcg ttcttgtcct cgcggagaga gcagggaagg ggcaagccta taatggtcca
      421 ggcctgcccc tttgttttgt gcggctgcca tcgtcacggc atgggacgca gccggcagcg
      481 tgcttacccc gcgatctcga gccccgcact cgcatagacg acgcctccat cgaccgcgat
      541 tgttgtcccg acaacgtagt cgccggcgcg cgaggcgaga tagatcgcaa cgcccgccat
      601 atcctcatcg gttcccacgc ggcctgccgg aacgcgcttt gcgacctcgt cggcctggtc
      661 gcgcgcggcg cggttcatgt ccgacttgaa tgctcccggc gcaatggcgg tgacgttgac
      721 atgatccctg atcagcttgg tcgccatgcg ccgcgtcaga tggatcactg cggctttgct
      781 cgcggcatag gaataggttt cgttcggatt gacgaagatg ccgtcaatgg aggcgatgtt
      841 gatcaccttg gctggacgtt cggcgctcgc ggccgcgcgc agcggtttcg ccagggcctt
      901 ggtcaggaag aacagcgcct tgacgttgag gtccatgacc ttgtcccagc cgttttccgg
      961 aaactcgtcg aaatccgcgc cccaggccgc gccggcattg ttgacaagga tgtcgagctt
     1021 cggctgcagc ttgatgattt cgccggcaag cttttcgcag cccgcgaccg tcgaaatgtc
     1081 tatcggcagt gcgatacatg cgccgccata ggtggcggtg agttccttcg cggtcgcttc
     1141 gcaggggccg gccttgcgcg cggtgatgta aaccttcgcg gcgccctgcg cgagaaaacc
     1201 cgccgcgatc atcttgccga tgccgcgcga gccgccggtg accagcgcgg tgcgtccctc
     1261 gagcgaaaac agatccttga acatgggtgc ctccctgcat gtccgtcatt gccgggttcg
     1321 acccggcaat ccatcttgaa gactcatttc gaagctggat ggatgcgcgg gtcaagcccg
     1381 cgatgacgcg tttttctttg ggtgagttcg ggcgacttac gccgcgtcca cgcggcgaaa
     1441 gccgttccac atcgcggtcg cggcgcccga ggtcaggacc ggaaggatcc gcgcgtcctg
     1501 gtaattgccg ttgagcgaaa cccgaggcag cgcggtcaga tgctgcccgc tttccggaaa
     1561 gatcataccc ggccgcgtcg tcaccgcggt cttgtagccg gccgcacgtg cgagcgcgaa
     1621 ctcgcgcgcg cccgccgcca gcctgtcgcc ataggggtag gcgaggtgca ggaccggtcg
     1681 ctgcagcgcc ctctcgaggc gcgtgcggct cgaattgagc tcttgtcgag ccttcccttc
     1741 gctctggtgc gccaaattgc aatggctgac ggaatgggcg ccgaccgtga ccaggggatc
     1801 ggcagcgaac gatttcagct cttcccagga catgcagagg tcgcggcaga tcgccgcatc
     1861 gtcgatgccg tggcgcatgc agagggcgtg gacctcgcgc tgcatatccg gttccggcag
     1921 cgtgcgcagc cagtcatgca ggcgttcgaa cgcggcctgc ttggccgcgg cgcccgcggt
     1981 gtcgaggcgg gtcggcgtcc cgcccatcga aagctcgatc gcggaggctc ttgcgatcgc
     2041 catttcgagc gcgatccacc acagatggcc cgttccttcc gcgaaatcgc tggcgacaaa
     2101 gagggtgaag ggcgcctcaa attcgcgcag cacgggcagc gcaaagtcgc ggttgtcgcg
     2161 atagccgtcg tcgagtgtga aacaggcgaa gcggcgcgag aaatttcgct cgagcagccg
     2221 cttgtgcacc tcgtccatgc tgatgatgtc gatggcctgt gtgcgcaaat gcgacagcat
     2281 cgcgcgcaga aagtcgggcg tcacttccag gtgccggttc ggctggaaat caccggtgcg
     2341 gccggggcgt acgtggtgca gcatgaaaat tgcaccgacg cccgaaaaga tcggccgcaa
     2401 caggacatgc gcccccgaga aatagagcgc tcccaagccg gcgcgtatga cggtattgcg
     2461 gaactgcttc attcaagaaa gctggctggc cggttgggat atgttttgag gaagttaacg
     2521 agacatcact gaagaaacgg ttagccggcg agagggaaag ccttaagtcc ggtttcgcca
     2581 tttccggttt gacagcccgc caagggtttg ttttctctct gtttcccagg acccgccgat
     2641 ttgcagttcc ttatcccctc aggacggccc ctccgcacgc aggacggtca ggtcggaaag
     2701 cggcactgga atcagagagt tggtggtccc tccgcgggat atcgaatgca cggaccgttc
     2761 aggtggagta gcaaatggtg gccgggtgtc atccctctgg tcgtcctttg ggcgttcgcg
     2821 gcatggacca ataccgaccc cgttgaagcc gatctcgcgg cgcgcagtac tgccgcgctg
     2881 aaggacgtcg tcctggacaa aaccagaatt gccgtcgaag ggcgcgacgt caccctcggc
     2941 gccgacgcct tttcggagga cggccgtcgc agcgcgctgg cttccgtcag agccgtgccc
     3001 ggggtgcggc tggtgaatga cgaaacgcgc ctggttcctg aggcaaaacc gttcgtctgg
     3061 tcggcggaac gggacgtcgt cagggtgacg cttggcggca gcgcgccgct gccggcggtg
     3121 agagccaagc ttgcagacgc cgcgcgcgcg ggcctcggcg gcgtcgaggt cgccgaccag
     3181 atgtcgctcg cacgcggcgc accgccgcgg ttcgagacgg ccgcgctgct cctgatcgac
     3241 cagatcggca agttgaagga cggcaagatc acgatctccg acaatgccgt gacgctgtcc
     3301 ggcatggcgc gcgagcttgg cggccgcgaa gcgatcgcgg ccagcctgaa ggatctaccc
     3361 gaggggtttt cggtcaaggc caacgacgtc aaggcgccgc cttacgtgtt ccaggcctac
     3421 aaggatcccg tcgcggtgac gctcacgctg tcgggcaacg tgcctgacaa caacattcac
     3481 gccgcaatcg cgcaggctgc ttcgcgaaaa ttctt
//
LOCUS       DATLJT010000293         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1394530,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000293 DATLJT010000000
VERSION     DATLJT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394530"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VM910_06635"
     CDS             complement(<1..302)
                     /locus_tag="VM910_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62253.1"
                     /translation="MISATRLTRRGFLHASGAAAAIAGTSLARPAIAQPAKVRYTLSW
                     LPTGQYAFLYCARQLGYFKQRGIELDIARGYGSMAAIQAVSTGQFEMGGAQTGANLL"
     gene            442..1356
                     /locus_tag="VM910_06640"
     CDS             442..1356
                     /locus_tag="VM910_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62254.1"
                     /translation="MPRVLTYGVPTDRCGLQLRLGVEKGFFRDEAIDLELRVVFGGPE
                     IAAELDAGRLLVGELGAPPGLTAMANRARFKIIGSGVRRGAVLYFVVCKQFSTFADLK
                     RARLGVLSKGSCSDWYMRELLRHRGLDPEADVTIVALGQRYPEVLTLLSDGDLDGAII
                     AEPHVSMGEVAGHFNVWLGVNACDFAPRMQWTIMVANDDLLLHEPDLIRGVLSACRRS
                     YRYAAKHREEWADFGARYFHIPRGTMTRSIERELPDLHFDCEVDVEGLAAAIALQQRL
                     GSVPASVRLSDIVDPRFTVFGAQPAAAG"
BASE COUNT          259 a          453 c          490 g          323 t
ORIGIN      
        1 agcaggtttg cgccggtctg cgcgccaccc atctcgaatt gaccggttga caccgcctgg
       61 atggccgcca tcgagccgta accgcgcgcg atatcgagct cgataccgcg ctgtttgaaa
      121 tatccaagct gccgggcgca ataaaggaac gcatactgtc cggtcggcag ccaggaaagc
      181 gtgtagcgaa ccttcgccgg ctgggcaatc gccggcctcg ccaggcttgt tcctgcgatc
      241 gcggcagccg cgccgctcgc gtgcaagaat ccacgccgcg tcagtcgtgt cgctgaaatc
      301 atggttgttc cctccgcttc cagctatatt cctagaagcc gccggagact tgcaagtgcg
      361 ttatatgcgc gcggcggcgg gtttcctgcc ggctttttag gcgtgggtcc agagcggttg
      421 ttttttgcag aggctgcatt catgccgaga gtcctcacct acggcgtgcc gacggatcga
      481 tgcggcctgc agctgcgact gggggtcgag aagggtttct ttcgcgacga ggcgattgac
      541 ctggagcttc gcgttgtctt cggcggccct gaaattgctg ccgagctcga tgcgggacgg
      601 cttcttgtcg gtgaattggg cgcaccccca gggttgacgg ccatggcaaa ccgggcccgc
      661 ttcaagatca taggcagcgg tgtgcggcgt ggggcagtac tctattttgt cgtatgcaaa
      721 cagttttcca cctttgcgga cctcaagcgg gcgcggctcg gcgtcctgag caagggaagc
      781 tgcagcgatt ggtacatgcg cgagctgttg cgccatcggg gccttgaccc cgaggctgat
      841 gtaaccatcg tagctctggg ccagcgctat cccgaggttc tcaccctgct atctgacgga
      901 gatctcgacg gcgccatcat tgcagaacca cacgtcagca tgggagaggt ggctggccat
      961 ttcaatgtct ggttgggggt caacgcctgc gatttcgccc cgcggatgca atggacgatc
     1021 atggttgcaa acgacgattt gcttctgcac gagcccgacc ttatccgcgg cgttctttcc
     1081 gcgtgtcgtc ggagctatcg ctatgccgcg aagcaccgcg aggaatgggc cgactttggc
     1141 gcccgttact ttcatatccc acgcggcacc atgacgaggt ccatcgagcg cgagcttcct
     1201 gaccttcatt tcgactgtga ggttgatgtg gagggactcg cagcggccat cgcgcttcag
     1261 caaaggctcg gcagcgtgcc agcctccgtt cgattgagcg acatagtgga tccgcgcttc
     1321 accgtatttg gcgcgcagcc agcggctgcg ggttgagcaa gcgcttgggg tctgacgcgc
     1381 gaatgagcgg gcatacgccg gcacgtaccg aagcgcacga ccgttggcac gcgacggccg
     1441 atgtggtcgt ggtgggctat ggttatgccg gtgccgtcgc cgccatcgaa gcgcatgatg
     1501 ccggcgccga cgtgctcctt atcga
//
LOCUS       DATLJT010000294         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2773642,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000294 DATLJT010000000
VERSION     DATLJT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773642"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..789
                     /locus_tag="VM910_06645"
     CDS             466..789
                     /locus_tag="VM910_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62255.1"
                     /translation="MRAVRAAPWSTWGASALGGVTRWLAHKTLDASSAPNARAAAVRS
                     VRAWGTACFVGERGQVTLDVSPDLLRRQVTLVGSWTFSKQGQAECAEFVAERKVDVER
                     LFTHR"
     gene            complement(1251..2843)
                     /locus_tag="VM910_06650"
     CDS             complement(1251..2843)
                     /locus_tag="VM910_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI62256.1"
                     /translation="MSAEVSDQRFNRALAAYLRQEVRAPAAAISDFLDIIIEDARGLQ
                     LDHVLADLDRMRSASVRLTTFVNSVAAEGSSEPGTGEEFEAFQRRLRHDLRSPLNVIK
                     GYSELLLEDMEADKEQPLRADLAKLRDSANQLLAQIDALVTLRREAAKPEGIGSLAQE
                     AGFVADVLRAVQPLQMSSAVPEAVQSSRILVVDDNAANRDVLARRLTREGHQVVTASN
                     GASALELIGSHEFDLVLVDLIMPLMNGFEVLRRLKATEHTQDVPVIVISALDELDSVV
                     RCIESGAEDYLTKPFNPVLLRARINASLEKKRLRDREKKFIHDLEQERQRSELLPLNI
                     LPQVVVERMREGENLIADHIMDATILFCDLVGFTALSQELSADRTVEFLSQIFSAFDR
                     LALEHGVEKIKTIGDAYMVAAGIPQPQADHVLRIVALAPRLLQSVNAIAAKTGLNLQA
                     RIGIHTGPLVAGVIGTHKFVYDVWGDTVNTASRMESHSLPGRIQISAATRAALGDRYS
                     YEPRGMMDIKGKGRMETYFLMV"
     gene            complement(2969..3343)
                     /locus_tag="VM910_06655"
     CDS             complement(2969..3343)
                     /locus_tag="VM910_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62257.1"
                     /translation="MPKVLLVEDNEMNRDMLSRRLTRRGFEVVFAADGQQGVDLARSE
                     RPDIILMDMSLPVMDGWEATRRIKADATMRTIPVIGLTAHAMSGDREKAIEAGCDDYD
                     SKPVEIDRLLGKINRLLNGDED"
     gene            complement(3312..>5301)
                     /locus_tag="VM910_06660"
     CDS             complement(3312..>5301)
                     /locus_tag="VM910_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62258.1"
                     /translation="IEAISEGFSLYDSEDKLIICNTRYKDFFSGHRDKMVPGTPFEQI
                     VRASLERDEIEDSKDDPEAWLATRMERHRNPSEPHIQRRSDGRWVRVSERLTANGGVV
                     ATYTDITMLKQREAELADLVHKLEIARDAANEASRTKSTFLANMSHELRTPLNAIIGY
                     SEILQEDVADLGQESLMTDLKKIEASGRHLLGLINDILDLSKVEAGRTELYIEDVDLA
                     SLFEEVQVIVAPLIQKNSNTLELRLADDLGSIRTDRTKLKQSVLNVLSNGNKFTENGR
                     LRVVAERFQADRPMVRIAISDTGIGMDEEQLGRLFQAFGQVDASTTKKYGGTGLGLAI
                     TRHFCQLLRGDITVKSKPGEGSTFTITLQDLGAKPEQTADAVAGVFDTAAEVDGASTV
                     LIVDDDPVARDLLSSSLKGKGYRIVHAGNGDEALNLARKLRPDAITLDIIMPQTDGWA
                     VLTALKGDAELRDIPVVIVTILQDRAIGLSLGAVDFLTKPVDRARLTALLHQLLRREG
                     LILLVEDESNTRAMLRQAIERMDLEVAEAVNGRGALEWLAQNQPPALILLDIMMPEMD
                     GFEFLDAFRKHPEWQDIPVIVLTAKELTAQERERLMGQTRKVIAKGDSFRDDIAAAIG
                     AAIRRPAAHAAAASSACLNQESKEKGLSNAKGAAGRRQ"
BASE COUNT          938 a         1644 c         1660 g         1059 t
ORIGIN      
        1 ggactcggtc accgcgatga cgtaaccatc atgggtgtgc cgggcgtagc catagctgcg
       61 cagatcggcc cgcatcaggc tgaggccggg gatatgccta gcgtgccaat agtgcgtgat
      121 ggctttcgga tctggttcca cgacgccgct gtaaataagg tgccgagcgt ggactcaaag
      181 gcatgcggcg caggaaggca aatcaatcga ctgcaatttt tgccaagaca gggcgggtgc
      241 cggtcatagt gggcaggtat tgtttcagga cagccggcca ccctaactga cattgtcacg
      301 ctgccgcgcg tcagcgaaac cctatcagaa cgccgcacac ggtcagaagt cctttgggac
      361 gacccgctgc tactagcgtc tgagtgagat ggaggtgacg gcggcgcgga gagcaatgcg
      421 caccgctaac taacgccatt agcaccgggt tcctgaagct gcggcttgcg cgccgtgagg
      481 gccgcgccct ggtctacgtg gggcgcgtcg gcactgggtg gggtcacacg atggctcgcg
      541 cacaagacgc tcgacgcctc ctcggcgccg aacgcccgtg ccgcggcggt gcgctccgta
      601 cgcgcgtggg gcaccgcctg tttcgtcggc gagcggggtc aggtcacgct cgacgtcagc
      661 ccggatctcc tgcgccggca ggtcacgctg gtgggatcgt ggacgttctc gaagcaaggc
      721 caggccgagt gcgccgagtt cgtcgccgag cggaaggtgg acgtggagcg gctcttcacg
      781 caccgctagc ggctcgaaca ggccgaggaa gcctaccggc tcttcgacac gcagacgacc
      841 ggcaaggccg tcatcctgcc gtcctgacgt cagtgagcgt agcccggatg agccataagc
      901 gcgttcacgc gcgtccatag cccgtaccta aagggcgttt acgcccgtct ttcgcgggcc
      961 taaagggcgt cgggttctgc cctccgagcc tggtctatcg gccgctgcac tcaggcggcg
     1021 gcacgacgtg accccgctcg gggtgcctga cgccggccat atccgaaaga gcattcgggc
     1081 gggatctccc ggatttcgcc atagcgcgtc gaagacgcgc gtgaacgcgc ttatggctca
     1141 tccgggctac ttgctgcgcc gatggtcagt gcgttcagcc aaaatcccgc acggctgttg
     1201 tgccagttca ttttgattgc caacatcgtt cgacctcggc atccacacga ttaaaccatc
     1261 aggaaatagg tttccatcct gcccttgccc ttgatgtcca tcatgccgcg cggctcgtag
     1321 ctgtagcgat cgccgagcgc ggcgcgggtt gcggccgaga tctgaatgcg gccgggaagg
     1381 gagtgcgact ccatgcggct tgccgtgttc accgtgtcgc cccagacgtc atagacgaac
     1441 ttgtgggtgc cgatcacgcc cgcgacgagt gggcccgtat gaataccgat gcgcgcttgc
     1501 aggttaagac cggtcttcgc ggcgatggca ttgaccgact ggagcaagcg tggcgcaagc
     1561 gcgacgatgc gcagaacatg gtccgcctgc ggctggggga tgccggccgc caccatatag
     1621 gcgtcgccga tagtctttat cttttccacg ccgtgctcga gggcaaggcg gtcgaaagcg
     1681 gaaaatatct ggctaaggaa ctcgacggtg cggtctgccg acaattcctg gcttagcgcg
     1741 gtaaatccaa cgaggtcaca aaacagtatg gtcgcgtcca tgatgtgatc ggcaatgagg
     1801 ttctcaccct cgcgcatgcg ctcgaccacg acttgcggca ggatattgag cggcaacaac
     1861 tccgagcgct gccgctcctg ctccaagtca tggataaatt tcttttcccg gtcgcgcagc
     1921 cgcttcttct ccagagaagc attaatgcgc gcccgcagca gcacgggatt gaatggcttg
     1981 gtgaggtaat cctcggcgcc cgattcaatg cagcgcacca cgctgtcgag ctcatcgagc
     2041 gcggaaatca cgatcaccgg cacatcttgg gtatgttccg tcgccttcag ccggcgcagc
     2101 acctcaaaac cgttcatcag cggcatgatg agatcgacca ggactaggtc gaattcgtgg
     2161 ctcccgataa gctccagcgc cgaagcacca ttggaggcgg tcaccacctg atgaccctcg
     2221 cgcgtaagcc gtcgcgcgag cacatcgcga ttggcggcgt tatcgtcgac taccaaaatg
     2281 cggcttgact gcaccgcttc gggcaccgcg ctgctcatct ggagcggctg cacagcgcgc
     2341 agcacatccg cgacaaaccc cgcttcttgc gcgaggctcc cgatgccttc cggttttgct
     2401 gcctcgcggc gtaaggtgac gagtgcatcg atctgggcaa ggagctggtt ggccgagtct
     2461 ctgagctttg cgagatccgc ccgcagtggc tgttctttgt ccgcttccat gtcctcgagc
     2521 aacagctcgc tgtaaccctt gatcacgttg agcggcgatc gcagatcatg gcgaagccga
     2581 cgctgaaatg cttcgaactc ctctcctgtc ccgggctcgg aggagccttc cgcggccacg
     2641 ctgttcacga acgtggtcag ccggacgctc gcgctgcgca tccggtcgag atccgcgagc
     2701 acgtggtcga gctggaggcc gcgcgcatcc tcaatgatga tgtcgagaaa atcggagatg
     2761 gcggcggccg gcgcccggac ctcctgccgc aagtaggcgg ccagggcgcg attgaagcgc
     2821 tggtcagaga cctcggccga caagaggctt ctccttcagc ttcgagaggt gcgcttcggg
     2881 cccgctcctc accctaactc agggtgagaa gccgtccact cgaggggcga gtgcagcgag
     2941 cgtctcaaag aacgtcagcg acacaacgct agtcctcgtc cccgttgagc aggcggttga
     3001 tcttgccgag cagacgatcg atctctaccg gcttgctatc gtaatcgtcg cacccggcct
     3061 cgatcgcctt ctcgcggtcg ccgctcatgg catgggctgt gagcccgatc accgggattg
     3121 tgcgcatcgt ggcgtccgcc ttgatgcgcc gggttgcctc ccacccatcc atcaccggca
     3181 ggctcatgtc catgaggatg atgtcgggcc tttcgctgcg cgcaaggtct acaccctgtt
     3241 gtccatccgc cgcgaacacc acctcgaagc cgcgtcgggt aaggcggcgc gagagcatgt
     3301 cgcggttcat ctcattgtct tcgaccagca gcacctttgg cattcgacag acctttttct
     3361 ttgctttctt gattaaggca tgccgacgaa gcggccgcgg catgcgcggc cggccgccgg
     3421 atcgccgcgc cgatggcggc ggcaatatcg tcgcggaagg aatcaccttt ggcgatgacc
     3481 ttgcgcgtct gacccatgag acgctccctc tcctgcgccg tgagttcctt ggcggtgagc
     3541 acgatcaccg gaatgtcctg ccattccgga tgtttgcgga atgcgtcgag gaactcgaag
     3601 ccgtccatct cgggcatcat aatatcgagc agaatgagcg cgggcggttg gttttgcgcg
     3661 agccattcca gcgctccgcg gccattgacg gcctctgcca cttccagatc catgcgctcg
     3721 atcgcctggc ggagcatcgc acgcgtgttc gactcgtctt cgacgagtag gatcaaaccc
     3781 tcgcgccgca acagctgatg caggagggcg gttagccggg cgcgatcgac tggtttggtc
     3841 aggaagtcga cggcgccgag tgagagtccg atcgctcggt cctgcaggat tgtcactata
     3901 accaccggga tatcgcgcag ctcggcatcg cccttcagcg cggtcagcac cgcccagcca
     3961 tcggtctgcg gcattatgat gtcgagcgtg atcgcgtccg gccgcagctt gcgcgcgagg
     4021 ttaagggcct cgtcaccgtt tccggcatgc acaatccgat atcccttgcc cttgaggctt
     4081 gagctgagca agtcgcgtgc gaccgggtcg tcgtccacga tcagcacggt gctcgcgccg
     4141 tcgacctcgg cggcggtgtc aaaaaccccc gccacggcgt ccgccgtttg ttcgggcttg
     4201 gcgccgaggt cctgcagcgt gatggtgaag gtggagcctt cgccgggctt actcttcacc
     4261 gtgatgtcgc ccctgagcag ctggcagaaa tgccgggtga tcgccaagcc gaggcctgtg
     4321 ccgccgtact tcttcgtggt cgaggcatcc acctggccga acgcctggaa caaacggccg
     4381 agctgctcct cgtccatgcc gatgccggtg tcagagattg cgatccgcac catgggccga
     4441 tcggcttgaa agcgctccgc gaccactctc agccggccgt tctccgtaaa cttgttgccg
     4501 ttgctgagta cgttgagcac gctctgcttc agcttggtgc gatcggttcg gatgctgccg
     4561 aggtcgtcgg cgaggcgaag ctcgagggtg ttgctgttct tctgaatgag cggagccacg
     4621 atcacctgca cctcctcgaa caaggaggcg agatcgacgt cttcgatgta gagctccgtg
     4681 cgccccgctt caaccttgga gaggtcaagg atatcgttga tcagaccgag cagatggcgg
     4741 ccagacgcct cgattttctt gagatcggtc atgaggcttt cttgtccaag gtcggcgacg
     4801 tcctcctgca ggatctcgct atagccgatg atggcgttga gcggcgtgcg cagctcgtgg
     4861 ctcatattgg cgaggaaggt cgatttggtg cggcttgcct cgttggcggc atcgcgggcg
     4921 atttctaact tgtggacgag atccgcaagc tctgcttccc gctgcttgag catcgtgatg
     4981 tcggtataag tcgccaccac gcccccgttc gcggtgagcc gttcgctcac gcgcacccag
     5041 cggccgtcgg agcgacgctg gatatggggt tcggacggat tgcgatgacg ctccatgcgc
     5101 gtggcgagcc aggcctcggg atcgtccttg ctatcttcga tctcatcccg ctccaagctc
     5161 gcgcgaacga tttgctcgaa gggggttccc ggaaccattt tatcgcgatg cccggaaaaa
     5221 aagtccttgt agcgcgtgtt gcagatgatg agcttgtctt cggaatcgta gagagagaat
     5281 ccctcggaga tcgcctcgat c
//
LOCUS       DATLJT010000295        11903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_935471,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000295 DATLJT010000000
VERSION     DATLJT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935471"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VM910_06665"
     CDS             <1..793
                     /locus_tag="VM910_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HVI62259.1"
                     /translation="GFTIEGSRIVEWSKRILADRDSMLQELSMLQNTLSGRIRLGAMP
                     TSSPVLPHINEHFLKLHPGVQVDVSFLGLDELRVKLINFELDVGITYTDQKQPAPLTS
                     FPLYKEHLSLLVPDNPRFPATTTTWSTVADLPICLLPAHMHERQIIDAAFASVGKTPT
                     PVIESDSIVNLAFHTMLGGAVTIVPSHFELVIGGFPGTRLVKLVEPEVVRQVGLIWVD
                     GDPMLPMAKAMLQSVKATPIPALLRPPAPRVAKQSHGHGPRSRAS"
     gene            complement(802..1011)
                     /locus_tag="VM910_06670"
     CDS             complement(802..1011)
                     /locus_tag="VM910_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62260.1"
                     /translation="MERTRLELAIEKNIRTPEVATLAYGAVDMARLNESIEQLGFAHG
                     FKTKFRAEDIFDPAFLPRASERRVQ"
     gene            complement(993..1451)
                     /locus_tag="VM910_06675"
     CDS             complement(993..1451)
                     /locus_tag="VM910_06675"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62261.1"
                     /translation="MVQPKDLKGKKLGAPAADLSYAQLPIFLRANSIEASAITVINIG
                     LPVREPMLAAGQLDAVIGISFETYVDMIERGVPVDDISMMLMADYGVHAYGSAIVINS
                     DSSSAKSDAIKAFLRAFLKALKSTIKAPVAAISSVVRRDAASTAKWSGHA"
     gene            1688..1972
                     /locus_tag="VM910_06680"
     CDS             1688..1972
                     /locus_tag="VM910_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62262.1"
                     /translation="MNGATGPASFPSSVNFSEVCAAAKSDTSSSVTKIAAATTTALFR
                     KRDSPTKAIVAVPAGKTSVRIRLLVVMPSDRPFVNNKGPSGHQSVYSREQ"
     gene            2282..3415
                     /locus_tag="VM910_06685"
     CDS             2282..3415
                     /locus_tag="VM910_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI62263.1"
                     /translation="MSGSFRARLFSVAAGLGCMLACAQPALSEEIKDYGKPGTPIELV
                     VGYQPYYTESWSGVIMRSKKFYEKYLPKGSKVDFQVGLQGAIIVNGMLAGKVDIGYVG
                     DMPAIVSTSHSNVRDIRIVSVLGLGYDQCNAFLVRVDAPKFQSPEEGIKWLDGKIVAV
                     PKGSCTDRFAQAVFKRFKIAPSEYLNQSIEVITSGFRAGKLDAAVMWEPTTSRLALDK
                     LARKVATGASVNENDGGFMVMPRALIEQRPDVVKAWLEAELDAQLYFGDEKNAMAVSA
                     MALEQTTGFGQRTLWYSAYGTYPKSEGGTTTRIMLPYTFTPEAKELIAKAAKFLVEIK
                     SIKADIRPEAVMPTFTEEILKSRNLKAPVGEVKALPDSAYTGS"
     gene            3516..4451
                     /locus_tag="VM910_06690"
     CDS             3516..4451
                     /locus_tag="VM910_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62264.1"
                     /translation="MKELEPMLAAGEINASLSDAPAGRSADGTDRVRRVKVAAYRLRQ
                     WLGSPRPYLMLLGFGLFFAFWYLTVEVWKLPRFSQMPGPTTVISEWLSPDPTYGLSIY
                     SPEYYQHIWVSWRRVSIAFLLATATGVPLGLFLGWSLRFKEYVFPVFELLRPIPPLAW
                     VPLAIIMFQGSETPIVFLTFLASFYATTLNTMLGVESIDVSFVRAAYCLGARRRQVFW
                     HVIVPGAMPFIFTGLQISVGVAWFSLVAGEMVSGQYGLGYLINTSYTMVQYPTMVIGM
                     ITLGVVGYATSAAVRIFGNYLMQWRARELAMGGEK"
     gene            4448..5344
                     /locus_tag="VM910_06695"
     CDS             4448..5344
                     /locus_tag="VM910_06695"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62265.1"
                     /translation="MNRAPLPTHDRPSAAAKAPVSPSPARHKGEIRIVNVVKIYGSET
                     ARIRAVDNCTFDVPAGKITVVVGPSGCGKTTLLNAIAGFHSITSGTIYLDDALLCGPG
                     RPSAAPGPDRIVVFQNGALFPWKTIIENVAFGPMMQGRLSKKQAFEKGRAMLAEAGLR
                     DLESNYPGEISSGMRRRVEITRALMNDPKVLLLDEPYRALDSLTKSVMHEALLETFDR
                     NQVTIFFITHDLEEAIFLGHRVVIMTTRPCRPKKILTVDIPHPRDYSVLTTKRFREYL
                     DETIAAVHEEALKAFAAGERER"
     gene            5346..6164
                     /locus_tag="VM910_06700"
     CDS             5346..6164
                     /locus_tag="VM910_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62266.1"
                     /translation="MPKPSALSGRLYQTVASTKFWRGVVATVVFIALWEIVSRVGISR
                     VPAPSAVVLTFGDLMADPSYWFSWYLSMRRVFLGFFAAMLVGIPLGLAMAVSKAFYGT
                     VFPVFEVLRPIPPLAWVPAAIIFWPTQELSIMFVTFLGAFYAVVINVIDGARAIDVRY
                     YQAARSMGSSGWDIFRRIILPGTVPSIVVGATVGIGIAWAVVVAAEMISGGGSAGSMG
                     GAGGGLGFFIWNSYVGGSYTQIVVGMISIGIAGYISSALLRKLGELATPWLREQ"
     gene            6187..7101
                     /locus_tag="VM910_06705"
     CDS             6187..7101
                     /locus_tag="VM910_06705"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62267.1"
                     /translation="MTVMPLPQAPHPSAAASAQRAATDTVAEIEVRDVSKSYGAAQFA
                     KTVVRDCSFTIDRGRLTVMIGPSGCGKSTLIRLLAGFERPTSGTIRIGGKSVTEPGRD
                     RLVLFQETALFPWMTAYDNIMFGPRARAEGGADARKRAELLLDKVGLGAFRRKYPTQL
                     SGGMQRRAELARAMINEPKVMILDEPFRGLDAMTKKLMQEYYAKLYEETRRTTFFVTT
                     DIDEAIFLADRILVMTNVPARVRAVLEVNLPRPRRLEDVIENDDANLIKMQVLSLLHE
                     EAMKSFAHGSKAAADFIDAYSRRIRKPS"
     gene            7168..8268
                     /locus_tag="VM910_06710"
     CDS             7168..8268
                     /locus_tag="VM910_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HVI62268.1"
                     /translation="MSETQDLAFSAPRSTPRRAALIEARSKTSSCAFQAEPQEPLLYA
                     ALRAGVAVPYECASGTCGTCKARRIAGVIVGDWGEAPGKAYLRPDRDEVLLCQTRALS
                     DCSFEIPGSVNLASMMRVRPYFGRGTVARVEALTQDTVELSLDLDPALEFDAGQFVVF
                     RVPSIAGYRGYSMVNHPGTAKQAKFVVKRKPAGAFTDWLFSRAGAGDPIEWFGPLGKA
                     IFTPQEQRTIVCIGGGSGIASIMSILEAGCSSRHFDHFEAAVYFGVRSNADVFYLDRL
                     HRFADAFPTMLRITVALSHDVPAAEIRERYPLLAFESGFPHEVAARDLAGRFAGRVAY
                     LAGPPILVDVSIRMLITQGRLPVRDIRYDKFS"
     gene            8294..8764
                     /locus_tag="VM910_06715"
     CDS             8294..8764
                     /locus_tag="VM910_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI62269.1"
                     /translation="MSLAAIESDNFEILRSTDILDGVSDATVKKIAAVARPQTVDEGE
                     TVYSVGDPVRSAYVVAAGRLRFVLGGDGRPAAQGTIVPAGDILGWAALLGDQPRRIAT
                     VIALEECRLLEIEGETLLQILDQDARAGYLVMRRLARMITQSFLEQSTLLKGAT"
     gene            complement(8772..9107)
                     /locus_tag="VM910_06720"
     CDS             complement(8772..9107)
                     /locus_tag="VM910_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI62270.1"
                     /translation="MGWQRLCSAQEVAIGRLTSAEVQGVKVLVTRVGSDFFALPPLCP
                     HMAEPLEISGVCADGMLTCTKHIWQWDLRSGEPRGEAEKPLLYYPTRLQEGDLWIDFD
                     HELTYDHDN"
     gene            complement(9151..9660)
                     /locus_tag="VM910_06725"
     CDS             complement(9151..9660)
                     /locus_tag="VM910_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI62271.1"
                     /translation="MNAMAKTEVKSVDQAIRDVIYRACLLLDQEKFVEWLALCAPEFS
                     YRITTYSPEIRRQMTWFEQDVAGLKAMIDLLPKHNTDHGRLTRHATVYAVEADPSQGE
                     ASATTSFACYRTMLDGINSHIDSGETQLFLIGKYYDRFQLGENGPRFIERNVVLDTRR
                     LDKGSHYPI"
     gene            complement(9691..10920)
                     /locus_tag="VM910_06730"
     CDS             complement(9691..10920)
                     /locus_tag="VM910_06730"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVI62272.1"
                     /translation="MTTQMAKTTYDSGEPIPYVDSRIYSDQSIFEEELEKIWKRVWLV
                     TVHESEIPEPLDFRTMTVAREPIVIVRGSDNKIRAFLNVCPHRGNLILRQPSGSLTVA
                     EPSGNANHMTCMFHAWQFDCFGRCVDIPRAKAGYQDRLRKEDIGLKALPCEVAHGGFV
                     WLSLDPNAQPLSAYIGQAFDIMREELDTEPLTIFHYHKAIIPSNYKLWHDTNSEFYHD
                     YMHYFNRATSMLQKGYFERQYTTFPNGHCTVGSMEVKYDAYQGAADRVLSFPGMPRNG
                     WKMVDLFPGATYNIRGSSLRVDTMTPIGPDRVMIEFRGLGLKRDTEDEQAQRVRDHNT
                     IWGPFGRNLHEDLLGITGQGVSMRPGSTPRHILHGRRENNSIHDEIGMRHFYTEWSRR
                     MERSPCDPFSDTLVAAE"
     gene            11208..>11903
                     /locus_tag="VM910_06735"
     CDS             11208..>11903
                     /locus_tag="VM910_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62273.1"
                     /translation="MFISNPFAELSASVPPSVMQAYVVVMAFLVAAGTLFDIVHKKSA
                     TYFFNSWRNSTHKAKQRVGGGEMLAIAVQMAVVDVMASGEFCNMRRRVAHLLTMYGFV
                     IYVVTTVIMVFAYPTPATPTPANLPTLWYIGALMVCLGGYWFWFFIRVDVAAEGNSPF
                     RFVRADLFIVSLVLSVTFGLVWACVQATGNIAWAYVFLALYLLTTTVLFGSIPWSKFS
                     HMFFKPAAALEKRV"
BASE COUNT         2247 a         3524 c         3583 g         2549 t
ORIGIN      
        1 gggtttcacg atcgagggca gccgcatcgt cgaatggtcc aaacggattc tggcagaccg
       61 cgactcgatg ctgcaagagc tctcgatgct gcagaatacg ttgtcgggac ggatccgatt
      121 gggagccatg ccgacaagct cgccggtgct gccgcacatc aacgagcatt ttctgaaact
      181 ccaccccggg gtacaggtgg acgtgagctt tcttgggctc gacgaattgc gggtgaagct
      241 gatcaatttc gaattggacg tgggaatcac ctacaccgat cagaaacagc ccgcgccgct
      301 gaccagtttt ccgctgtaca aggagcattt gagcctgctg gttcccgaca atccacgctt
      361 tccggccacc acgaccacat ggtccacggt tgccgatctg ccaatctgcc tgctgccagc
      421 gcacatgcac gaacggcaga tcatcgatgc cgcgttcgcc tccgtcggga agaccccgac
      481 gcccgtgatc gaatcggatt cgatcgtcaa cctcgcgttc cacacgatgc tcggcggagc
      541 ggtcaccatt gtgcccagcc atttcgaact cgtcatcggc ggatttccgg gcacgcgtct
      601 cgtcaaactg gtcgagcccg aggtggttcg acaggtcggg ctgatctggg tcgacgggga
      661 cccgatgttg ccgatggcga aagcgatgct ccagtcggta aaggccacgc cgattccggc
      721 gctgctgcgg ccacccgctc cgcgggtcgc gaagcagtcg cacggacacg gcccgcgctc
      781 tcgcgcgtcg tgagggctcg atcactgcac ccggcgctcg ctagcgcgcg gcagaaacgc
      841 gggatcgaaa atatcctcgg ctctgaattt ggtcttaaag ccatgagcaa aaccgagctg
      901 ctcaatagac tcattgagac gggccatatc gacggcgcca taggcaagcg ttgccacctc
      961 gggagtgcgg atatttttct cgatggcgag ctctaggcgt gtccgctcca cttcgctgtt
     1021 gaggcagcat cgcggcgaac cacggaggag atcgcggcca ccggagcctt tatcgtgctc
     1081 ttcaaagctt tgagaaaagc ccgcaggaac gccttgatgg cgtcgctctt tgcggaggag
     1141 gagtccgagt tgatcacgat tgcgctgccg taggcatgaa cgccgtaatc agccatcagc
     1201 atcatgctga tatcgtcgac aggcacgcca cgctcgatca tgtcgacgta ggtctcgaat
     1261 gagatgccga ttacggcatc gagctggcca gccgcgagca tcggttcgcg caccggcaac
     1321 ccgatattta tcaccgtgat ggcggacgcc tcgatgctgt ttgcccggag gaagataggt
     1381 aattgagcgt agctcaggtc ggcggcgggc gcgcccagct tcttgccttt gaggtccttc
     1441 ggttgcacca cgccccgggc tttttcgtcc gatgacggca tagggcggcc ggttgttgac
     1501 catgaacact gcccgagccc gcatttctgg attctgatcc ctcgaccgga tgagtgcatt
     1561 aatatcgaca aaacccatat cgtattcacc cgacgcgatg cgtttcaatg catcggccga
     1621 atcccgtgcg gtatcgatag tcacattcaa ctcttccgcc gcgtaatatc ccttgtcgat
     1681 gccaacggtg aacggcgcca cgggtcccgc tagtttcccc tctagtgtga acttcagcga
     1741 ggtttgcgcc gcggcaaaat ccgacacaag cagcagcgta acgaagatcg cggcggcaac
     1801 cacgactgcg ttgttccgca aacgggattc accgaccaag gccatcgttg ctgttcccgc
     1861 agggaaaaca tctgtgcgga tcaggttact ggtggtgatg ccctccgatc gccccttcgt
     1921 caacaacaaa ggcccaagcg gccatcaatc ggtctattca cgcgaacaat gagcgcgatc
     1981 tggattttcg atcggccttg tctatcattt gagagtagtg tgtttgcctt ccgcgctgat
     2041 agacgccgga aattacctca ttcaccgcca tcgtttgaca gccacaatcg ccgtccatag
     2101 gctttaataa aacgcgaacg ccccgaaggg cgcgttgtcg gtacctcagt tacggttccg
     2161 cgatctcacc agggaggacg aaatgaattc tcatggcatg gccgcacagc cgcggccgtc
     2221 tcctcgattc gaggaggagt cgctttgtaa tgctccgcgt gtcgcaacga gctcctcgcg
     2281 aatgtcggga agcttccgag cgcgtctctt ttcggtcgcg gccgggctcg gctgcatgct
     2341 ggcttgtgcc cagccggcat tgtccgagga gatcaaggac tacggcaagc cgggcactcc
     2401 gatcgagcta gtggtgggtt atcagcccta ctacacggag tcgtggtccg gcgtcatcat
     2461 gcgcagcaaa aagttctacg agaaatatct tccaaagggc tcgaaggtcg atttccaggt
     2521 aggcctgcag ggcgccatca tcgtcaatgg catgctcgcc ggcaaagtcg acatcggcta
     2581 cgtcggcgat atgccggcaa tcgtgtccac cagccattcg aatgtccgtg acattcgcat
     2641 cgtgtcggtg ctcgggctgg gttacgacca gtgcaacgcg ttcctggtgc gcgtcgacgc
     2701 gcccaaattc caatcgccgg aagagggcat aaagtggctc gacggcaaga tcgtcgcggt
     2761 gccgaagggg agttgcaccg atcgcttcgc gcaagccgtc ttcaagcggt ttaaaatcgc
     2821 tccctccgaa tatctcaatc aaagcatcga ggtgatcaca agcgggttcc gcgctggcaa
     2881 actcgatgcc gcggtgatgt gggagccgac gacatcccgg ctcgcgctgg ataaactcgc
     2941 ccgcaaagta gccacgggcg cctccgtcaa tgagaacgac ggcggcttca tggtgatgcc
     3001 gagggcactc atcgagcagc gtcccgacgt cgtaaaagcg tggctcgagg ccgagctgga
     3061 tgcgcagctg tatttcggcg acgagaaaaa cgcgatggcg gtaagcgcca tggcgctcga
     3121 gcaaacgacc gggtttggcc aaaggacgtt gtggtattcc gcctacggga cctacccgaa
     3181 atcggaaggc ggcacgacca cccgcatcat gttgccctat accttcacac cggaggcgaa
     3241 ggaactgatc gccaaggcgg ccaagttcct cgtcgagatc aagagcatca aagcagatat
     3301 ccgcccggaa gcggtgatgc cgacattcac tgaggagatt ctcaagagtc gcaacttgaa
     3361 ggctccggtc ggtgaggtga aggcactgcc ggactccgcc tacactggct catgaacgac
     3421 ggcactctcg tttcgccgaa acattcgggt tcccactctc ggcctctcgg ccggacccgg
     3481 tcgggtggct gggtcggcac agtgagaggg acggcgtgaa agagctagaa ccgatgcttg
     3541 ccgccggcga aatcaatgcc agcctgagcg acgccccggc gggtcgctct gcggatggca
     3601 ctgatcgggt gcggcgggtc aaagtcgcgg cctatcgcct gcgacaatgg ctgggcagtc
     3661 ctcgcccgta tctgatgctg cttgggttcg ggcttttctt tgccttctgg tatctgacgg
     3721 tcgaggtttg gaagctcccg cgcttttcgc agatgcccgg gccgaccacg gtcatcagcg
     3781 aatggttaag tccggatcca acttacggac tttcgatcta ttcgccggaa tattatcagc
     3841 acatctgggt cagctggcgg cgagtttcca tcgcctttct gcttgccacg gcgaccggcg
     3901 tgccgctcgg actcttcctg ggatggtcgc tgaggttcaa ggagtatgtc tttcctgtgt
     3961 ttgaactctt acgcccgatc ccgccgctcg cctgggtacc gttggcgatc atcatgttcc
     4021 agggatcgga gactcccatc gtattcctga cgttcctcgc ctccttttat gcgacgacgc
     4081 tcaatacgat gctcggggtc gaatcgatcg acgtgtcctt cgttcgcgcc gcttattgtc
     4141 tcggagcaag aagacggcaa gtgttttggc acgtgattgt gccaggcgcg atgcctttca
     4201 tcttcacggg actgcagatt tcggtaggcg tcgcgtggtt ttccttggtt gccggcgaga
     4261 tggtatcggg ccagtacggc ctcggatatc tgatcaatac gtcctacacc atggtgcagt
     4321 atccgacgat ggtgattggc atgatcaccc tcggggtggt ggggtacgcg accagcgcag
     4381 cggtgcgcat tttcggcaat tacctgatgc aatggcgggc gcgcgagttg gccatggggg
     4441 gcgaaaagtg aatcgcgcgc cgctgccgac gcatgaccgt cccagcgccg ctgcgaaagc
     4501 gccggtatcc ccatcaccgg cacgccacaa gggcgagatc cggatcgtca atgtcgtcaa
     4561 gatttacggt tcggaaacgg ccaggatccg tgcggtggac aattgcacgt tcgacgttcc
     4621 tgccggaaag attacggtcg tagtggggcc ctctggatgc ggcaagacca cgctcttgaa
     4681 cgcgatcgcc ggcttccaca gcatcacgtc ggggaccatt tatctggatg atgcattgct
     4741 gtgcgggccc ggccggccga gcgcagcacc gggaccggat cggatcgtgg tgtttcagaa
     4801 cggtgcgctg tttccctgga agacgattat tgagaacgtg gcgttcggtc caatgatgca
     4861 gggccggctc agcaagaagc aggccttcga gaagggccgc gcgatgctgg cggaagcggg
     4921 gctgcgggat ctcgagagca actatccggg tgaaatttcc tccggcatgc gccggcgtgt
     4981 cgagatcaca cgcgcgctga tgaatgatcc gaaggtactg ctgcttgatg aaccctaccg
     5041 cgcgctcgat tcgctgacca agtcggtgat gcacgaggcc ttgctcgaga cgttcgacag
     5101 gaaccaggtt accattttct tcatcactca cgatctcgag gaggcgattt ttctcggaca
     5161 tcgcgttgtg atcatgacga cgcggccctg ccgcccgaaa aagattctca cggtcgatat
     5221 cccgcatccg cgagattaca gcgtactgac cacgaaacgg ttccgcgagt acctggatga
     5281 gactatcgcg gcggtgcacg aggaagcctt gaaggctttt gcggcgggcg agcgggagcg
     5341 ttgaaatgcc gaagccgagc gctctctcag gacgcctcta ccagaccgtc gccagcacca
     5401 agttctggcg cggagtggtg gccaccgtgg tgttcatcgc gctttgggaa atcgtatcgc
     5461 gcgtgggcat ctcgcgggtt ccggcgccca gcgccgtcgt gctgactttt ggcgacctta
     5521 tggctgaccc gagctattgg ttcagttggt acttgagtat gcgaagagta ttcttgggct
     5581 tcttcgccgc gatgctggtg ggcattccgc ttgggctggc catggccgta agcaaggcgt
     5641 tctacgggac cgtgtttccg gtcttcgagg tgctgcgacc gattcctccg ctggcttggg
     5701 tcccggccgc gatcatcttc tggccgacgc aggaactatc gatcatgttc gttacgttcc
     5761 tcggcgcgtt ctatgcggtg gtcattaatg ttatcgatgg tgcacgggcc atcgacgtgc
     5821 gctactacca agcggcacga tccatgggat cgtcgggctg ggacattttt cggcgcatca
     5881 ttctgcccgg aacggtgccc tcgatcgtcg tcggcgccac ggttggcatt ggcatcgctt
     5941 gggcggtcgt cgtcgccgcg gagatgattt ccgggggcgg cagcgccggc tccatgggtg
     6001 gcgccggcgg cgggctcggc ttctttatct ggaattctta cgtgggcgga tcctacacgc
     6061 agatcgtcgt cggcatgatc agcattggca ttgctgggta catctcgagc gcgctattgc
     6121 gcaagctcgg cgagctggcc accccctggc tgcgcgagca atgatacatc gagccggacc
     6181 agtgcgatga ccgtcatgcc tttgccgcaa gctccccatc cttcggctgc cgcgtcggcc
     6241 cagcgcgctg cgactgatac cgtcgccgaa attgaggtcc gcgacgtcag caaatcatac
     6301 ggcgccgccc agtttgcaaa gactgtagtt cgcgattgct cgttcacgat cgatcgcggc
     6361 aggctcacgg tcatgattgg cccttcgggg tgcggcaaga gcacgctcat tcgcctgttg
     6421 gcgggcttcg aacggccaac ctcgggcacg atcaggatag gcggcaaatc ggtcaccgag
     6481 cccgggcgcg atcggctggt gctgtttcaa gagaccgcct tgttcccctg gatgaccgcc
     6541 tacgacaaca tcatgtttgg tccgcgggcg cgcgccgagg gcggggcgga tgcgcgaaag
     6601 cgagcagagc ttctgcttga taaggtcggc cttggcgctt ttcgtcgcaa gtaccccacc
     6661 cagctctcgg gggggatgca gcgtcgcgca gagcttgccc gcgcgatgat caacgagccg
     6721 aaagtcatga tcctcgacga gccgttccgc ggactcgacg ccatgaccaa gaagctgatg
     6781 caggaatact acgccaagtt atacgaagag acgcggcgca ccacgttttt cgtcaccacc
     6841 gacatcgacg aggcgatctt tctcgcagat cgaattttgg tcatgaccaa tgtaccggcg
     6901 cgagtacgcg cggtgctgga ggtgaatctg ccgaggcccc gtcgcttgga ggacgtgatc
     6961 gagaacgatg atgccaacct gatcaagatg caagtgctgt ccctgctgca tgaggaggcg
     7021 atgaaatcgt tcgcgcacgg cagcaaggcg gcagcagatt tcattgacgc ctactcgcga
     7081 cgcatccgca agccgagctg acatgcccgc cctttccgtc acacgccctc gctcggccca
     7141 tttcgcggct atgcccggac tttccacatg agcgaaaccc aagaccttgc cttttctgcc
     7201 cctcgctcca cgccgaggag ggccgcgctg atcgaagcgc gatcgaagac ctccagctgt
     7261 gcgttccaag ccgagccgca ggagccgctc ctctatgcgg ccttgcgcgc gggtgtagca
     7321 gttccctacg agtgcgccag cggcacctgc ggaacgtgca aggcacggcg tattgctggc
     7381 gtgatcgtcg gcgactgggg agaagcgccc ggcaaggcct atctgcgccc tgatcgcgac
     7441 gaggtgctac tctgtcagac ccgcgcgctc agcgactgca gcttcgaaat tccgggcagc
     7501 gtcaatttgg cgtccatgat gcgggttcgc ccgtacttcg gtcggggcac ggttgcccgc
     7561 gtcgaggcgc tgacccagga cacggtcgag ctgtcgcttg atctcgatcc tgcccttgag
     7621 ttcgatgccg gccagttcgt cgtattccgg gttccgtcca ttgccggtta ccgcggctat
     7681 tcgatggtca atcatcccgg gactgccaag caagcgaaat tcgtggtcaa gcgaaaaccg
     7741 gccggcgcgt tcaccgactg gctgttctcg cgcgccggcg ccggcgatcc catcgaatgg
     7801 tttggtccgc tgggcaaggc gatcttcacg ccgcaggagc agcggacgat tgtctgtatc
     7861 ggcggtggct ccggcatcgc ctccattatg tcgattctcg aggccggatg ctcctcgcgg
     7921 catttcgacc acttcgaagc cgcggtctac ttcggcgtcc gcagcaatgc cgatgtgttc
     7981 tatctcgatc gactgcaccg cttcgcagac gcgttcccga ccatgttgcg aattacggtc
     8041 gcgctgtccc acgatgttcc agcggcggaa attcgagagc gctacccgtt gctggcgttc
     8101 gagtctggat tcccgcacga ggtcgctgcg cgcgatctcg ccggaaggtt tgcgggacgc
     8161 gtcgcctatc tcgctgggcc gccgattctg gtcgacgtgt cgattcgaat gctgatcact
     8221 cagggtcggc tgccagtgcg cgacattcgc tacgacaagt tcagctaatc ccgagtgagt
     8281 tggagagacg agaatgtcct tagctgcgat cgaatccgac aatttcgaga ttctgaggtc
     8341 caccgacata ctcgatggcg tatcggatgc gaccgtcaag aagattgcgg cagtcgccag
     8401 acctcagacg gtggacgaag gggagaccgt ctactccgtc ggcgatcctg tgcgcagcgc
     8461 gtacgtggtt gccgccgggc ggctgcgatt tgttctcggg ggcgacggtc gtcccgcggc
     8521 gcaaggcacc atcgttccag ccggcgacat tctcggctgg gccgcgcttc tgggcgatca
     8581 gccgcggcgc attgccaccg tcatcgcgct ggaagaatgc cggctgctcg aaatcgaagg
     8641 tgagacgcta ctgcagattc tggatcagga tgcgcgcgcc ggctacctgg tcatgcggcg
     8701 tctggccagg atgatcacac agagctttct cgagcagagc acactgctca agggcgcgac
     8761 gtgactgaac ttcaattgtc atgatcatac gtgagttcgt gatcgaaatc gatccagagg
     8821 tcgccttctt gcagccttgt cggatagtag aggaggggct tctcggcctc gcctctcggc
     8881 tcgccgcttc gcaggtccca ttgccaaatg tgcttggtgc aggtcagcat cccgtcggcg
     8941 cagactccgg aaatttccag cggttcggcc atatgcgggc aaagcggcgg aagcgcgaag
     9001 aaatcactcc ccaccctggt gaccaggacc ttcacgcctt gaacttcggc gctggtgagc
     9061 cgcccgatcg caacctcctg cgcagagcaa agcctctgcc aacccaattg ctagctccct
     9121 tgcgttttgg atcaccgcaa ccaacacggc tcagatcggg tagtgcgacc ccttatcgag
     9181 gcggcgtgtg tcgagaacca cgtttcgctc gatgaagcgc gggccgttct cgcccagctg
     9241 gaaccggtcg taatactttc caatcaggaa cagctgcgtc tcgcccgagt cgatgtgtga
     9301 gttgatacca tcgagcatgg tccggtagca ggcaaaggag gtcgtggcgc tggcctcgcc
     9361 ctgcgatgga tcggcctcca ccgcgtagac cgtcgcgtgc ctggtgagcc ggccgtgatc
     9421 cgtattgtgc ttcggcagca ggtcgatcat ggctttgagc ccagccacgt cttgctcgaa
     9481 ccaagtcatt tgcctgcgga tctcggggct ataggtggtg atgcggtagc tgaactccgg
     9541 cgcgcacagg gcgagccatt ccacgaactt ctcttggtcc agcaagagac aggcacgata
     9601 gatgacatcc cgaatcgcct gatcgacgga tttcacctcg gtttttgcca tggcgttcat
     9661 gatccaccac cttagccgca gcgccgcgat ctattcggcg gccaccaacg tgtcggagaa
     9721 cggatcgcag ggcgatcgct ccatccggcg cgaccactcg gtatagaagt gtcgcatgcc
     9781 gatttcgtcg tggatggaat tgttttcgcg ccggccatgc aggatatgcc gcggcgtcga
     9841 gcccggccgc atgctgaccc cctgtccggt gattcccaag agatcctcat gcaggttgcg
     9901 cccgaacggt ccccagatgg tattgtggtc gcggacgcgc tgcgcctgct catcttcggt
     9961 gtcgcgctta aggccgagac cgcggaactc gatcatgacc cggtctggtc cgatcggggt
    10021 catcgtgtcg acgcgcaatg acgagccacg gatgttatat gtggctcccg ggaacaaatc
    10081 gaccatcttc cagccgttgc gcggcatgcc gggaaacgac agaacgcggt cggccgctcc
    10141 ctgataggcg tcgtatttca cctccatcga tccaacggtg cagtgcccgt tggggaaggt
    10201 cgtgtactgt cgttcgaagt atcccttctg cagcatgctc gtggcgcgat taaagtaatg
    10261 catgtagtcg tgatagaatt cgctgttggt gtcgtgccag agcttgtaat tactcggtat
    10321 gatggccttg tgataatgga agatcgtgag cggctcggtg tcgagctcct cccgcatgat
    10381 atcgaaggcc tggccgatgt atgcagacag tggttgcgca ttgggatcca atgacagcca
    10441 gacgaagccg ccgtgcgcga cctcacaagg gagtgcctta aggccaatgt cttccttcct
    10501 tagccgatcc tgatatccgg ctttcgcgcg gggaatatca acgcatcgtc cgaagcagtc
    10561 gaattgccaa gcatgaaaca tgcacgtcat gtggttggca tttcccgacg gttcggccac
    10621 ggtcaggctt cccgaaggtt gccgcaggat tagattgccg cgatgtgggc atacattcag
    10681 gaaagcccga atcttgttgt ccgagccgcg tacgatgacg atgggttcgc gggcaacggt
    10741 catggtccgg aaatcgagtg gttcgggaat ttccgattcg tgcacggtaa cgagccatac
    10801 tcgtttccag atcttctcaa gctcttcctc gaaaatactt tgatcgctgt agattcggct
    10861 gtcgacatac ggaatcggct caccgctatc gtaggtggtc ttcgccatct gcgtcgtcat
    10921 gacaattctc cgttgcgtgc cagcactcgc aatcgcaatt tgcggtctct gttccgggct
    10981 tgagtccagc ggccaaacga gaagctttcg ccagtatagc cgttcccggt ttcaccacaa
    11041 aaattcatac catacatacc gcaatagttc gggtctatcg cgagccgggt gccgccgata
    11101 tacgtgaact atcagacaat tcgggcggca agcggttgaa gcgccgagtt gcacgcgact
    11161 atagtgcttg ccgtactctt ggagatcctt ccagacaagc gtctaccatg ttcatcagca
    11221 acccgttcgc cgaactttca gcgtccgtgc cacccagcgt catgcaggca tacgtcgtcg
    11281 taatggcctt cttggtcgcc gctgggacgc ttttcgacat cgtgcacaag aagagcgcga
    11341 cgtacttctt taacagttgg cgcaattcca cgcataaagc gaagcagcgg gtcggcggcg
    11401 gagagatgct ggccatcgcg gtccagatgg ccgtagttga tgtcatggct tcaggtgaat
    11461 tctgcaacat gcggcgcaga gttgcccatc tcctgacaat gtacggcttt gtgatttacg
    11521 tcgtcaccac cgttatcatg gtcttcgcgt accccacgcc tgccaccccc acgcccgcca
    11581 atttaccgac attgtggtac atcggggccc tgatggtttg tctcggcggc tactggttct
    11641 ggtttttcat ccgagtcgat gtcgccgcag agggaaattc accgttccgt ttcgtgcgtg
    11701 ccgacttgtt tatcgtctcg cttgtcttga gcgtgacctt tggcctggtg tgggcgtgcg
    11761 tccaggcgac gggtaacatc gcttgggcat acgtgttcct ggctctgtat ctcctcacca
    11821 ccacggtgct atttggatca attccgtggt ccaaattctc gcatatgttc ttcaagcctg
    11881 ctgctgccct ggagaaaaga gta
//
LOCUS       DATLJT010000296         5379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2543807,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000296 DATLJT010000000
VERSION     DATLJT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543807"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VM910_06740"
     CDS             <1..271
                     /locus_tag="VM910_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62274.1"
                     /translation="LAAHDAIPAMYPNRVYVAAGGLMSYGNDVPDGYRRAGLYVARIL
                     KGEKPADLPIDQATRFEFVINMQTARLLGIEVPNSIQLLADEIIE"
     gene            complement(420..1391)
                     /locus_tag="VM910_06745"
     CDS             complement(420..1391)
                     /locus_tag="VM910_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI62275.1"
                     /translation="MRAACDFNPVRFSTWDLPERERLSRWREEFGRGIVSVDVEPVAS
                     DRPFYAEATLQALPGVRTALCAGSLARLNRTRALVADGDDSVGLVVSLSAKANIFQRG
                     ANVTLDAGDAIPLSNDDVGIVTSTRHLGILLPRGPLAGRVSNLDGAFMRLIPRSTGPL
                     RLLVGYLDLLRKESTLATDELRRRVADHVHDLAALALGASRDTQQNALSAVAAARLAA
                     ALAHIAESFFEPGLTVEAVARRQGVSRRYLQRLLEESGMSFTARVNELRLQKAFAMLM
                     NEQHGKQRISDIAMEAGFSDISHFNRLFRARFGDTPSGVRGGGDAGQ"
     gene            1641..1856
                     /locus_tag="VM910_06750"
     CDS             1641..1856
                     /locus_tag="VM910_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62276.1"
                     /translation="MTDIDRLIDELARRLRGRPMLEVMTAARKIENRVRDLYLNEALR
                     KYAADRHKEPSRHVVAALHRLLVPYSF"
     gene            2231..3322
                     /locus_tag="VM910_06755"
     CDS             2231..3322
                     /locus_tag="VM910_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI62277.1"
                     /translation="MRGTSRERSMEKAAKSGRRGKVRHLALQPDAMGVATRWAAVRLR
                     EAGVVLEPLLRSAGLSASQINRKGIRIGVASQIKFLELAAKALNDPLLGFRLTRDADF
                     RLAGLLYYVAASSQTLGDALDRAERYSSIVNAGVVLKCFEARNLTIALRYAGVARHSD
                     RQQMECLATAIIRFCRDSTERRLNPIAVHFVHRRLPQSSEIEKFFGCRIEFAANTDRI
                     IFDKKAKQLRLVAADPHLNELLLHYCEQALAYRRAREGPLQIAVENAIAPLLPHGKAQ
                     LGAVAQTLGVSSRTLARRLAAEGLSFGEILSQLRSDLATHYLSDPNLPISEIAWLLGY
                     KSVSAFSHAYKRSTEMNPSKMRDKLLASH"
     gene            4416..4610
                     /locus_tag="VM910_06760"
     CDS             4416..4610
                     /locus_tag="VM910_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62278.1"
                     /translation="MNPHVTLDAALTKRTPETKGEVRLLALWPFILVVAILAIVALVS
                     DASLTADQRIQVFQQAGFYP"
BASE COUNT         1063 a         1608 c         1631 g         1077 t
ORIGIN      
        1 gctggcggca catgacgcca tcccggcgat gtaccccaac cgcgtatatg tcgcggcggg
       61 cgggctgatg agctatggca acgacgttcc agacggctat cgccgcgctg gactctacgt
      121 cgcgcggatt ctcaagggcg agaagcccgc cgacctgccg atcgatcagg ccacgagatt
      181 cgagttcgtg atcaacatgc agaccgcccg gctgctcggt atcgaggtgc caaattcgat
      241 ccaattgctc gcagacgaga tcatcgaata accatacagc ttttgctgca ctgcatgagt
      301 ctgcttttgg cacaaggcgg acatagccgc gtgatgacgc tgcgtccgct tgtgacccaa
      361 agaacgagag atatcgcagg gccggcgtct ccggcatcgg gtgcaagcag ccggccgcgt
      421 cattgacccg catcaccgcc gccgcgtacg ccgctcggcg tatcgccgaa acgggcacgg
      481 aacagccggt tgaagtgcga gatgtcggaa aatcccgctt ccatggcgat gtcggaaatc
      541 cgctgcttcc cgtgctgctc attcatcagc atggcgaatg ccttctgtag gcgcaattcg
      601 ttcacccgcg ccgtgaacga catgcccgac tcttcaagga gccgttgcag gtaacgccgc
      661 gaaacgccct gccggcgcgc gactgcttcc acagtcagtc ccggttcgaa gaagctttcg
      721 gcgatgtgcg caagcgccgc ggcgagccgc gctgctgcta ctgcgctgag ggcattctgc
      781 tgcgtgtctc gactggcgcc gagcgccaag gcggcgagat catgaacgtg atcggccacc
      841 ctccgacgca actcgtctgt ggcgagcgtg gattccttgc gaaggaggtc gagatagccc
      901 acgagcagcc gcaacgggcc ggttgaccgc gggatgagac gcatgaacgc gccatcgaga
      961 ttgctgacgc gccctgcgag cgggccgcgc ggaagcagga tgccgaggtg gcgcgtgctc
     1021 gtcacgatgc cgacgtcgtc gttggagagc gggatggcgt cgcctgcgtc gagcgtaacg
     1081 ttcgcacccc gttgaaatat attggccttt gcactgaggc taacgaccag cccgactgag
     1141 tcgtcgccgt cggccacaag tgcgcgcgtg cgattcaaac gcgcaagcga gccggcgcac
     1201 aaggcggtgc gcaccccggg cagggcttgc agcgttgctt ccgcatagaa tggccggtcc
     1261 gacgcgaccg gctcgacgtc gacgctcaca atgccgcgcc caaactcttc tcgccaccgc
     1321 gacagtcgtt cgcgctctgg caagtcccag gtcgagaagc gaaccgggtt aaaatcgcag
     1381 gcggcgcgca tgcggcatcc cattcctaag cttcgatcga atgcgaagtg aacagaagcg
     1441 catcctccat attgaccgcg ctcgatgtgg aggatttgtt attcttcgcc aaacatttgt
     1501 tcgtcgctgc caggatccgt gcggacaaac caggaggccg ctgcactgcg ggatttcggt
     1561 ccacgtatga ccgcgttggc tcagtcgcgg gaacacacgg ccatgggatg cgttttctct
     1621 ggacgtgaag gacgaacacc atgaccgaca tcgacaggct gatcgacgag ctggcccgcc
     1681 gacttcgcgg gcggccgatg ctggaagtca tgacggcggc gcggaagatc gagaaccggg
     1741 tgcgcgacct gtacctgaac gaggctttga gaaagtacgc tgccgaccgt cataaggaac
     1801 catcgcgcca cgtcgttgca gcgctccatc gcctactcgt tccgtacagc ttttaggtca
     1861 tctataccgc cgtttagttt agctcgggcg ctcggctatg tggcggagtg ctcgaacctg
     1921 tcttaacgca tcgcaacgca gtgccattta ttcgattacc tcgttggcgc ggccaacagc
     1981 gtgggcgcgc cgttacggcc cagctccttc gcagccctga gagcgaagtg ctttacagtg
     2041 atcactcatc gctccaacaa gtttgggctg cagtggcttg acggacggga gagtcgtttc
     2101 gccgcggacg cagagcaagc tccttagcgc tgacagagcg ggggatcttt tgctataagg
     2161 agcggaacca gcgaccttgt tatttgctgc tattgattgc tcctgcgtgc cgcggtcaag
     2221 agtcgttccg atgcgcggaa caagtcgcga gcgctcgatg gaaaaggcgg caaagtccgg
     2281 gcggcggggc aaggtgagac accttgcttt gcagccggac gcgatgggcg tggcgacgcg
     2341 atgggcggca gtacgcctgc gcgaggcagg cgttgtgctc gagccgctgc tgagaagcgc
     2401 cggcctgtct gccagtcaga tcaataggaa aggcattcgg atcggcgttg cgagccagat
     2461 caagtttctg gagcttgcgg ccaaagcatt gaacgatcca ctcctcggct tcagactgac
     2521 gcgcgacgcg gacttccggc ttgccgggct gctctactac gttgccgctt cctcgcagac
     2581 gctcggcgac gcgctggatc gcgccgagcg ctacagttcg atcgtcaatg caggcgtcgt
     2641 cctgaaatgt tttgaagccc gcaatctcac gattgccttg cgttacgccg gtgttgccag
     2701 acattccgat cggcaacaga tggaatgttt ggcgacagcg attatccgct tctgtaggga
     2761 ttcaacggag cgccgtctaa acccgatcgc cgtccatttc gttcatcgac gcttgcccca
     2821 gtcctctgaa atcgaaaaat tctttggatg ccggatagaa tttgctgcga acacagaccg
     2881 gatcatcttc gacaaaaaag caaagcagct tcgtctcgtc gccgcggatc cgcatctcaa
     2941 cgagttgctg ctccactatt gcgagcaggc gctggcatat cgccgcgcca gagagggtcc
     3001 actgcagatc gctgtcgaaa atgcgatcgc tccgctccta ccgcatggca aagcgcagct
     3061 cggcgcggtt gcgcagacgc ttggggtgag cagccgaacg ttggcgcgga ggctggctgc
     3121 ggaaggcctg agcttcggtg aaattctcag tcaactccgc tccgatctcg ccacgcatta
     3181 tctgagtgac cccaaccttc cgatctctga gatagcatgg ctcctcggct ataaatctgt
     3241 tagcgcattc tcgcacgctt acaagcggtc gactgagatg aatccgagta agatgcgcga
     3301 caagttgctg gcaagccatt aacggagtgc cgacgggcgc gacgcggaag tgcgcaaaca
     3361 cgccgcggaa ttggttgcgc ttgatccgaa ggttatcctg gcctgggcat cgcgccgttt
     3421 cattgcgatg accccaagcc caaggaacat gcatcgcagc aaaagcggcc cgctcctgtc
     3481 cgctttgggt caaaagcgac ccaggcggac gaagccacac gatcatttat gtccgctttg
     3541 cctcggaaag cgcaaatgtc ccgagcgttc gatagcagga ggctatcagc gggtgctgct
     3601 tcgagggttc gcaaattgca aacactcgat gcgcgctgca ccaagacatt tccccgacgt
     3661 ccgcataggt tgattccgtt gcttcatgtt cctgcgcgac aagtcggtct tttcccgact
     3721 cacaattggc gctcatgcga acgtctctca aatccaactg gtcggaaaaa gatcggctta
     3781 cgtacgtcaa ttggatgcgc ggaattgcag ttttctatgg atgcatggcg ttccttgtct
     3841 ttgcgctttt ggtgctcacc aagccgtcga gcgttgcgcc gaatgagccc agggatcggc
     3901 agacctggtc cgtcgacttg gagggcgagc agacagacca caacgccgat gtatcgtgga
     3961 aaacgcgctt caagggcgaa aaccacagga atcggtagcg tcatgactaa gatctgccgc
     4021 gagggggcgt gaaatgttga cgtgcctgtc gcctttggcc gtcttgcttg cgctagtttt
     4081 gccgttgctg gccgttcgag aagcgcgtgc gcaaccgcgg gcagatacgc cggatgccac
     4141 caacgtctat ccgggtcgtt acgtcgtcgt ctgcaagccg gcgccaatca tcggttgttt
     4201 gtgctccagc gatgcgcagg acgagacgct ggtatttcct gagcttaccg gcgcggccca
     4261 ccatcgttcc aaggacgcaa gggatacaga gtatttgcaa atggtcacgt ggatgcgacg
     4321 gacttgcatc tcactcagcg gcccgacaag cgttgaatga tccatcgttt ccaaccttac
     4381 tcggaccgag tgagttatca aaaaacgagg ccaacgtgaa tccgcacgta actctcgacg
     4441 ccgccctgac aaagcgcacg ccggagacta aaggtgaagt tcgtcttttg gcgctctggc
     4501 ctttcattct tgttgtcgcg atccttgcga ttgtcgctct ggtatcagac gcgtctttga
     4561 cggctgatca gcggattcag gtgtttcagc aggctgggtt ctacccgtga gcgcgcaatc
     4621 cagcctagca cgcgctgatc gtgtcgacgg ttgtcgccgt ccagatcgac ggagcaacag
     4681 ccgcgacccc cggatcggga agctcaacgc gacacagagg ggatgcaaat ggtcgcgtgg
     4741 ataatcgccg gcgtggtcac gattggccta atctggtcgg tcgccgtgat gtcgaacttg
     4801 ctccccgtgg acggctcgat ttcggcggcg atatcgcggc ctgctaagta actcgccgcc
     4861 taacaccggg catcgactgc ttccggccgt cgttttatgg ccgcccgatc caccatggct
     4921 gcgacgaaca aatctttggc gtaaatgaac aagactcagg acgtaggcaa agcgaccaag
     4981 acgtgttcgg ccctgacgcg acgatgccga gcggggagag ccgaatgctt aagcttctcc
     5041 aactccgctc tgccaacttg ccccggccgt ttaagggctg gggcctttcc ccccgtccgg
     5101 ggcgacactt gctgcgtgtg ctagcctttc ggtcccaggc acgggaggtg ccgactgcta
     5161 cgcctcgatt cagaacgatt caggtctagc ccaaggactt accggctggc ccgtggcagg
     5221 ctgagcgtgc ggtaaacccg cgtcgtgaca ggggaggaag tccccatgtt ccgcataagg
     5281 cggcgcgagt tcatcaccct cctcggcggc gcggcggcgg cgtggccgct cgcggcacac
     5341 gcgcagcagc ggccaagcaa gttctcgcgc atcggtttt
//
LOCUS       DATLJT010000297         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2314111,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000297 DATLJT010000000
VERSION     DATLJT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2314111"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VM910_06765"
     CDS             complement(<1..549)
                     /locus_tag="VM910_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVI62279.1"
                     /translation="MGRLVIASNRVGSSLKGNEGGLATAMLAAFDQREVIWLGWSGEV
                     TDEPSGPTRVRREGRLTRMLVDLERSDYEGYYLEFANSVLWPVFHYRVNLAEYGRERW
                     AAYGRVNEMFAERVVPALKPDDILWVHDYHMIPLGSALRRLRVDNPIGFFLHIPFPAP
                     EVLATLPVHEALVRALFAYDLVG"
     gene            complement(1161..1862)
                     /gene="rpiA"
                     /locus_tag="VM910_06770"
     CDS             complement(1161..1862)
                     /gene="rpiA"
                     /locus_tag="VM910_06770"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HVI62280.1"
                     /translation="MSVDRDQLKRAAALRAIEEVQDGMVVGLGTGSTAAHVVEGLAAR
                     VAGGLRVVGIPTSERTAAQARRLGIPIATFAEHQRLDLTIDGADEVQLGTLDLIKGLG
                     GALLREKIVAAASDRLVIVVDQEKLVEQLGEHTPIPVEVTQFGWQATAVALAKLGCVP
                     ERRYMAGEQPYVTDGGNFIMDCRFGPLADPGITEQQLAMTVGTVESGLFVGRTSVVVV
                     ASMTGVEVLVAPPRK"
     gene            complement(1859..2542)
                     /gene="rpe"
                     /locus_tag="VM910_06775"
     CDS             complement(1859..2542)
                     /gene="rpe"
                     /locus_tag="VM910_06775"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVI62281.1"
                     /translation="MSRPIIVAPSILSADFGRLADEVRAVDAAGADWIHVDVMDGRFV
                     PNITIGPVVVESVRKATAKPVDVHLMIVEPERYLNDFAQAGADHLLVHCEPSSTIHLH
                     RTLAHIRDLGKVAGVVLNPATPLSLIEYVLDLCGIVLVMTVNPGFGGQQFLPEMLAKI
                     RALRCLCDERGLDPVIEVDGGLSGENAWQVSEAGATAIVAGSHIFHAPDYARAISAIR
                     NSSRRPLQS"
     gene            complement(2560..>3855)
                     /gene="zwf"
                     /locus_tag="VM910_06780"
     CDS             complement(2560..>3855)
                     /gene="zwf"
                     /locus_tag="VM910_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVI62282.1"
                     /translation="ATEPFDKEAWEFVASRISHFAGDLTQSDTYARLHDTLEKTAAQH
                     QTGRNAVFYLAVAAQLFGTIVEHIGAVGLTEEKDGCFRRVIIEKPFGTDLASARALNA
                     RILKVLSESQIYRMDHFLGKETVQNIMVLRFANGVFEPIWNRDHIDHVQISVAETVGV
                     ELRARFYEQTGALRDMVPNHVFQLLSMTAMEPPNSFEADAVRTEKHKVLEAVHPLDGE
                     EVRRNVVRGQYSPGRLNGDSVPAYRGEPGVSPDSNTETYVAMQLGVDNWRWAGVPFYL
                     RTGKRMTRRTTEIAIQFKQAPFALFRDTPVDILTPNVLALQIQPDEGASLQFGAKIPG
                     PEISLGGVRMNFSYKDYFNTEPTTGYETLVYDCMIGDAMLFNRADGVEAGWEVVQPIL
                     DLWHDDKTVPLETYAAGSAGPEAADNLLWRSGRQWRPIT"
BASE COUNT          740 a         1226 c         1161 g          728 t
ORIGIN      
        1 cccgacgaga tcataggcaa acagcgcacg caccaacgct tcgtgcaccg gcaatgtcgc
       61 cagtacctcg ggcgccggga atgggatgtg caggaagaat ccaatcgggt tgtcaacacg
      121 caaccggcgc agcgccgatc cgagcggtat catgtgatag tcgtggaccc acagaatatc
      181 gtcgggcttc agagccggga cgacacgctc ggcgaacatc tcgttgaccc gaccgtaggc
      241 cgcccaccgt tcccgaccat actctgcgag gttcacacga tagtggaaga ccggccagag
      301 cacgctattg gcgaattcga gataataacc ctcatagtct gagcgctcta ggtcgaccag
      361 catgcgggtg agccttccct cgcggcgaac gcgcgtgggg cccgacggct cgtcagtgac
      421 ctcaccgctc caacctaacc agatcacctc gcgctgatcg aacgccgcca gcatcgcggt
      481 cgcgagcccg ccttcgttgc ccttgaggga cgatccgaca cggttcgacg cgatgaccaa
      541 tcgtcccatc caagcctcca tgctgcggca atccgggcta ccgccagcgc tttcgcattt
      601 actatatcac ggtgttaacg ctccgggccg aatgtcgtta ccacaatctt gacattttct
      661 gcgcggggcc agctccgctc cccgggcctc tcgcggtccg ccgaactgaa attccgtttc
      721 ccgtgttttc ttgctgacca ttgtcgcgag gagacgacgg tcatgatgat taagactacc
      781 agtttggcct tcgcagctgc gtgcctgatg acggtttcgg cgatgccagc cgctcacgcg
      841 cagatagccg gcgggaccca agtgataacc aatgggccgc agaccgaccc gggcgatgtc
      901 tcgtcacctt ggtcggcccg gcagaacgtg atcgagagcc aacgttatga acggctcctt
      961 caaacgagcc ctggctttcg ccaggcgcgg atgcaaaagg agtgcggtcc gatcaccgac
     1021 ccgcaacttc atgccgattg tctggcgagc tttgatcaga acgtcggctc ttcgacagga
     1081 cccggaagct acccgagcgg atccggacgc tagatcgtcc ggcaccctct gatcggaagc
     1141 cgcccgcagc gggcggcttc ttactttctt gggggcgcga ccagcacctc gacacccgtc
     1201 atcgaggcga cgacgacaac cgatgtgcgc ccgacgaaga gcccgctttc cacagtaccg
     1261 accgtcatcg caagttgttg ctccgtgatg ccgggatcag cgagcggccc gaagcggcaa
     1321 tccatgatga aattgccgcc gtcggtgacg tatggctgct cgccagccat gtagcgccgc
     1381 tcgggaacac agccgagctt ggcgagtgcc acggcggtgg cttgccagcc gaattgggtg
     1441 acctcgactg gtatcggggt gtgctcgccg agttgttcga ccagcttctc ctgatcgacg
     1501 acgatgacca gccggtcgct ggctgcggcg acgatctttt cgcgcagcag cgcgccgcca
     1561 agacccttga tcaagtcgag ggtgccgagc tgcacctcat cggccccgtc tatcgtcaga
     1621 tccaagcgct ggtgctcggc gaacgttgct atcgggatcc cgagcctgcg ggcttgggcg
     1681 gcggtgcgtt cggaggttgg tatcccgacc actcggagcc ccccggcgac gcgcgcggcg
     1741 agcccttcga cgacatgagc ggcggtcgaa ccggtgccga gcccaacaac cattccgtct
     1801 tggacctcct cgatcgcgcg caacgcggcc gcgcgcttta gttggtcgcg atcaacgctc
     1861 acgactgcaa gggccgccgg ctcgaattgc gaatagctga gatcgcacgg gcatagtccg
     1921 gcgcgtggaa tatgtgagat ccggcgacga tggcggttgc gccggcctcg cttacctgcc
     1981 aggcattttc gccgctcaat cccccgtcta cctcgatcac ggggtcgagg ccgcgttcgt
     2041 cgcacagaca ccgcaacgcg cggatcttcg ccagcatctc tggcaggaat tgctgcccac
     2101 caaagcccgg attgacggtc atgacgagga cgatcccgca aagatccagc acgtactcga
     2161 tcagcgacag gggggttgcg gggttgagca ccaccccggc aaccttgccg aggtcgcgga
     2221 tgtgcgccaa ggtccggtgt aggtggatcg tcgagctcgg ctcgcagtgg acgagcagat
     2281 gatcggcgcc ggcttgcgca aaatcgttga ggtagcgctc gggctcgacg atcatcaaat
     2341 gcacgtcgac aggcttcgct gtcgctttgc gcacggactc gacgacgact gggccgatcg
     2401 tgatgttcgg cacaaagcgc ccatccatca catcgacatg gatccagtca gcccccgccg
     2461 cgtctaccgc ccggacctca tcggcgagcc ggccgaaatc ggccgacaga atagacgggg
     2521 caacgatgat cggacggctc atatgacccg attccgcgcc taggtgatcg gtcgccactg
     2581 ccggccgctg cgccacagca gattgtcagc tgcctcagga ccggcgctcc ccgccgcgta
     2641 agtttcgagc gggacggtct tgtcgtcgtg ccatagatcg aggatcggct gcaccacctc
     2701 ccacccggct tcgaccccgt cagcccggtt gaacagcatc gcgtcgccga tcatgcagtc
     2761 gtagaccaag gtctcgtagc ctgtggtcgg ctcggtgttg aaatagtcct tataagagaa
     2821 attcatccga acgccgccga gcgaaatctc cggccctggg atttttgccc cgaattgcag
     2881 cgatgcccct tcgtcgggct gaatctgcaa ggcaaggacg ttgggggtca gaatatcgac
     2941 tggagtgtcg cgaaacagcg caaacggcgc ttgcttgaat tgaatagcaa tctcggtggt
     3001 gcgccgggtc attcgcttcc cggtgcgcag ataaaacggc actccggccc agcgccaatt
     3061 atcgacgccg agctgcatcg caacgtaggt ttcggtgttg ctgtcgggag acacgcccgg
     3121 ctctcctcgg taggcaggaa cggagtcgcc gttgagccta ccggggctat actgcccacg
     3181 aacgacgttc cggcgcacct cctcgccatc cagcgggtgc actgcctcga gcaccttgtg
     3241 cttttcggtg cgcaccgcgt cggcttcgaa ggagttcggt ggctccatcg cggtcatgct
     3301 gagaagctgg aagacatggt tcggcaccat gtcgcgtaag gcacccgtct gttcataaaa
     3361 tcgggcccgc agctcaacgc cgacggtttc ggcgacactg atctgtacat ggtcgatgtg
     3421 gtcgcggttc cagatcggct cgaacactcc gttcgcaaaa cgcagcacca tgatgttctg
     3481 gaccgtctcc ttgccaagga agtgatccat ccgatagatc tgcgattccg ataatacttt
     3541 gaggatgcga gcgttcagcg cgcgggccga cgcaagatcg gtgccgaacg gcttttcaat
     3601 aatgacacga cgaaagcagc cgtctttttc ctcagtgagg ccaacggcgc cgatgtgctc
     3661 gacaatcgtc ccgaacaatt gggcagcgac agccagataa aagactgcat tgcggccggt
     3721 ttgatgctgc gctgcggtct tttccagagt gtcgtggagc cgcgcatagg tgtcggactg
     3781 ggtcaggtct ccggcaaaat gcgaaatgcg gctcgcgacg aactcccacg cctctttgtc
     3841 gaacggctcg gtcgc
//
LOCUS       DATLJT010000298         7212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1165711,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000298 DATLJT010000000
VERSION     DATLJT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165711"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VM910_06785"
     CDS             <1..444
                     /locus_tag="VM910_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVI62283.1"
                     /translation="KNFTDSDGNLKELAKALIAAEESWTPERTKLKRPSEWLISALRL
                     TGAQWAVGRVMSGQALLGEPLWRPPAPNGFADDESTWIEGLSQRVDIASNFAGRVADR
                     LDPVALVETGLGPLASAETRNTVARAESRAQALALLLMAPEFLRR"
     gene            448..1695
                     /locus_tag="VM910_06790"
     CDS             448..1695
                     /locus_tag="VM910_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVI62284.1"
                     /translation="MTRLHAPSRRKLLLASGSLFAWAYLPKLARAEGRDPRFLTIVLR
                     GALDGLATVAPVGDPDWVSLRGDNALTLDSKTSALKLDDFFALNPAMPNLHRMFKANE
                     AIVVHAAATSYRERSHFDGQDVLESGVEKPGAIRTGWLNRLLVGLAPEDRVGSNGGRA
                     FAVGPIAPLVVRGPAPVLSWWPRRVLPASDDTILRLLDLYRHTDVNFARVIEGRTKLS
                     AIAPAGSMEQKPVGQIAGPDFAQLRAYFAEAVGTAAKFLAQPDGPRVGALALNGWDTH
                     YNEGIATGWLAQLLGALDGALAAVQENMGPAWRETVVVLVTEFGRTARINGTNGTDHG
                     TATVMLVAGGALKGGRVIADWPGLKAADLLENRDLKPTIDVRAVLKGLLKDHLRASDH
                     ALAADVFPGSEDVRPIAGLVASS"
     gene            complement(2531..4057)
                     /locus_tag="VM910_06795"
     CDS             complement(2531..4057)
                     /locus_tag="VM910_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI62285.1"
                     /translation="MLVLLVLAGLPVAVWLDLRNMSERALTEQANELSSTIDSLRNSY
                     ASRVVGRVLANSEKTHVLPNYADVPGAIPIPATLSLELGDLINRNNGNTQFRFFSDYP
                     FKNRPPHAFDDFERRALESLRQNPHARVSEVSGSIFDRRVRLATPIIMAAACVSCHNS
                     HPDSPKHDWQVGDVRGIEEFIISQPIGAHIFAFKYLLLYFAFVAVSGLAFIGLQRYQS
                     SVIARFNRELARSNEFLSSLARKIAKYLPPQLYRGIFAGKDVEIATERKKLTVFFSDI
                     VDFTATAERMQPEELTALLNEYLTEMSSIAAAHGGTVNKFIGDAILVFFGDPESRGAV
                     KDAKACLAMALDMQRRLAELNVRWRQRGIEEPFRVRMGINTGFCNVGNFGSDDRMDYT
                     IIGAEANLAARLQSIAEPGGIVLSYETFMLVRDMVRARPLEPITLKGIAHPVVPYAVE
                     GSTAAARKAAPVISEHDTGLDLFIDTGAIDASAAERVRRTLENVAAELKLRSRTAEQN
                     "
     gene            complement(4315..5136)
                     /locus_tag="VM910_06800"
     CDS             complement(4315..5136)
                     /locus_tag="VM910_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate 1,2-dioxygenase"
                     /protein_id="HVI62286.1"
                     /translation="MFAASHGPIIARDWETMPRDHRNRLAAAFDEIGRRLKACRPDLL
                     VVISPDHWVNFFISNLPAVCIGIGDEHDGPPEPFMKKVFPRERLKGHAAFGRHLLKTA
                     LNGDFEPALSHRLKLDHGTCIPLWRIGVDAELPIVPVIVNDLEEPMPSIRRCLAWGRL
                     LRQAIESYPEPLRVAMLGTGGLSHSIGEPTMGWIDEEFDHACIKHFEDGEDARLAAFL
                     TDALARTGNGAHEIRDWVIAHAAAGSKGFELIDYFPSPQTLVGAGFASWRVTGSA"
     gene            complement(5147..5422)
                     /locus_tag="VM910_06805"
     CDS             complement(5147..5422)
                     /locus_tag="VM910_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62287.1"
                     /translation="MTDYWVSKLFFDLQHDSKLAAEYRANMPAVLERYEINPDARKAL
                     LEDDVGKIAPLVNAYLLRFYFQIRGMPEGEFIARLHALKAGKGKVDG"
     gene            complement(5521..6528)
                     /locus_tag="VM910_06810"
     CDS             complement(5521..6528)
                     /locus_tag="VM910_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62288.1"
                     /translation="MRLSRLIFASVAAAALIGASAAQSAEPVKIRISWVAPVTNWASI
                     MLEKKDLARHLGKSYTLEPVRFAGTPPMVTAIANGELEVSNLAYSTLAIAIENAGMDD
                     VRIIADEFRDGVEGYYSQEYMVLADSPIKKVDDLKGKVVMTNAAGSAVDVAMRAMLRK
                     FGLEDKRDYTIVESAFPTMRAMLSEKKVDMIPAVIPFSYDPELRKIGRTLFVQRDAIG
                     VTDMIVWTARKPFLDKNRAAMVDFMEDTLRITRWYLDPNNHKEAMEIAGRVTKQPPER
                     FDWLFTKRDAYHDPNMLPDLVALQKNVDMTRDLGFVKSAPDVKKHTDLSIVEEAAKRL
                     K"
     gene            complement(6666..>7212)
                     /locus_tag="VM910_06815"
     CDS             complement(6666..>7212)
                     /locus_tag="VM910_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62289.1"
                     /translation="FSAYFVNYGLSIWLPTLYRTVFKLPLDVSLRYGLITQAVGLLGT
                     LICALTIDHVGRRAWFAFSFAAATLALATLALFSAPSAEQVLTFMTIAYFFISTINIG
                     VYLYTPELYPTRVRAIAVGTATAWLRFASIIGPNVVGMMLAGGLPSVFATFATVAAVA
                     AVITGLYAVETKGRVLEEASP"
BASE COUNT         1261 a         2225 c         2383 g         1343 t
ORIGIN      
        1 aagaatttta ccgacagtga cggcaatctg aaggagctgg ccaaggcgct gattgcggcc
       61 gaggaatcat ggacgcccga gcgcaccaag ctcaagcgtc cctctgaatg gcttatatcc
      121 gcgctgcgcc tcacgggtgc gcaatgggcg gtcggccgcg tcatgtcggg acaggcgttg
      181 cttggcgagc cgctgtggcg tccgccggct ccaaacggct tcgccgatga cgagtcgaca
      241 tggatcgagg gcctgtcgca acgcgtcgac atagccagca atttcgccgg ccgcgtggcg
      301 gaccggttgg atccggtcgc gcttgtcgag acggggctcg gcccgctggc atcggccgag
      361 acgcgcaaca cggtcgctcg cgcggaaagc cgcgcgcagg cgctcgcctt gctgctgatg
      421 gcgccagagt ttctccggag atgaatcatg accagactcc acgcaccgtc gcgccggaaa
      481 ttgctgctcg cttcgggcag tcttttcgcc tgggcgtatc tgccgaagct cgcgcgtgcc
      541 gaagggcgcg acccgcgctt tcttacgatc gtgctgcgcg gtgcgctcga cgggctcgcg
      601 acggtggctc cggtcggaga tccggattgg gtttcgctgc gcggcgacaa tgcactgacg
      661 ctcgacagca agacatcagc actcaagctc gacgatttct tcgcgctcaa tccggcgatg
      721 ccgaatctgc accgtatgtt caaagcgaac gaggcgattg tcgtccacgc cgctgccacg
      781 tcctatcgcg agcgctcgca cttcgatggc caggacgtac ttgagagcgg ggttgaaaag
      841 ccaggcgcga tccgcaccgg gtggctcaac cgtttactgg tgggccttgc gcccgaggac
      901 cgcgtcggct cgaacggcgg cagggccttc gccgtcggac cgatagcgcc gttggtcgtt
      961 cgtggaccag cgccagtgct gtcttggtgg ccgcggcgag ttctgccggc aagcgacgat
     1021 acgatcctgc gcttgctgga cctctatcgg cacactgacg tcaacttcgc gcgcgtcatt
     1081 gaaggccgca ccaagctctc ggccattgct ccggccggca gcatggagca gaagccggtt
     1141 ggccagattg ctggccccga cttcgctcag ttgcgtgcct atttcgccga ggccgttggc
     1201 accgccgcaa agttcctggc gcagcccgat ggtccgcgcg tcggcgcgct cgcgctcaac
     1261 ggctgggaca cacattataa tgaaggcatc gcaactggct ggctggcgca gctgctgggc
     1321 gcgctcgatg gggctctcgc cgcagtccag gaaaacatgg gaccggcgtg gcgcgagacc
     1381 gtggtggtgc tcgtcaccga gttcggccgc acggcacgca tcaacggtac gaacggcacc
     1441 gaccacggca ccgccacggt catgctggtc gccggcggcg cgctcaaagg cggtcgcgtg
     1501 atcgccgact ggcccgggct gaaggcggcg gacttgttgg agaaccgcga cctcaagccg
     1561 accatcgacg tgcgcgccgt gctcaaagga ttgctgaagg atcatcttcg agcgagcgac
     1621 cacgcactcg cagccgatgt gttccccggc agcgaagatg tgagacccat tgcaggcttg
     1681 gtcgcgtcga gctgacgcag tgagtagagg ccgttctcca ggggggcgga atgctcaaga
     1741 gcagctgtgt tgctcccgta tgttccgtgc gtcccactga gaatccacct ggagcaaacc
     1801 gccacacgcg gccgctttgg gcagagttca tttttgcgct cgacagctgg ttgcgaaggc
     1861 gggagggcgt gttcgagtac agccagaggc cggactgcat tcttcgggcc gagctcagcc
     1921 gattgtccag tgatgtgctg ctttccgacg gaacgttcgg tcacgcgggt gaccgagtga
     1981 tcgaccttca cctctggaat gagcagatcc ctgtcatacc tctagcaggc tattccctgg
     2041 catgggggtg ccgcttcaac cggagcctcg caaaatccct tcgcggatta gctcaatttc
     2101 tcacgagcag gccgcaactc tcggacataa acatcatccg cgcagttaca aatctcgacc
     2161 tgcttcatcg tatcgccgcg cgacatggat tcgagaccgt tcgcgacccg gttaggcttt
     2221 caccttggga acacgcgcat cggttcggcc agaatattct gtattggctg ttgacgcttg
     2281 cgtgtcattc cggccgtacg cggccgcgga gattttcgcg aagtcggcag gtcatatatc
     2341 tttctcgcag agtactcgat tgcaagtaca ttgccacgac gagggggcac gaccacgatg
     2401 atgcccatgc agtcgctgat agtctcggca tagtttcacg aaatactgtt tgtgcccgaa
     2461 atgctgaggc ggtggcgccg agcacggcgg gaaaggaagg cgtggccgca tcagcggcgt
     2521 gagcgatcgt tcaattttgt tcggccgtac ggctgcgcag cttcaactcg gcggcgacgt
     2581 tttcgagcgt ccgccgcacg cgctccgccg ccgaggcgtc gattgcgccg gtatcgatga
     2641 agaggtcgag cccggtatca tgctcgctga tgactggcgc agctttgcgc gccgcggcgg
     2701 tgctgccttc cactgcgtag ggcacgaccg gatgcgcgat gccctttagc gtgatcggct
     2761 ccagcgggcg cgcgcgcacc atgtcgcgca cgagcatgaa ggtctcgtag ctcagcacga
     2821 tcccgccggg ctccgcaatc gactgtagac gcgccgcgag attggcctcg gccccgatga
     2881 tcgtgtaatc cattcggtcg tcgctgccga aattgccgac gttgcagaag ccggtgttga
     2941 tgcccatccg cacgcggaag ggctcctcga tgccgcgctg tcgccaccgc acgttgagct
     3001 cggcgaggcg gcgctgcata tccaacgcca tcgccaggca ggcctttgcg tccttgaccg
     3061 ccccgcgcga ttcgggatca ccgaagaaca cgaggatcgc gtcgccgatg aatttgttga
     3121 ccgtgccgcc gtgcgccgcc gcgatcgacg acatttcggt cagatattcg ttgagcagcg
     3181 ccgtgagttc ttccggctgc atacgctcgg cggtcgccgt gaagtcgacg atgtccgaga
     3241 agaagaccgt cagcttcttg cgctcggtgg cgatctccac gtccttaccg gcgaaaatgc
     3301 cgcggtagag ttgcggcggg agatattttg caatctttct tgccaagctc gagaggaact
     3361 cgttgcttcg cgccaactcc ctgttgaagc gtgcgatcac gctggattgg taacgttgca
     3421 gcccgatgaa ggcgagaccg ctgacggcga cgaaggcgaa ataaaggagc agatacttga
     3481 acgcgaagat gtgggccccg atcggctggc tgatgatgaa ctcctcgatg ccgcgcacgt
     3541 cgccgacttg ccagtcgtgt ttggggctat cggggtgcga gttatggcaa ctcacgcagg
     3601 cggccgccat gatgatcggg gtggccagcc ggacgcggcg gtcgaaaatc gagcccgaaa
     3661 cctcggacac gcgcgcatgc ggattttgcc gcaggctctc gagcgccctt cgctcgaagt
     3721 cgtcgaaggc atgcggcgga cgattcttga aaggatagtc cgagaaaaag cggaactgcg
     3781 tgttgccgtt gttgcggttg atcaggtcgc cgagctcgag cgagagcgtg gccgggatcg
     3841 gaatggcgcc ggggacgtcc gcatagttgg ggaggacgtg cgtcttttcg ctgttggcga
     3901 gcacgcgacc gaccaccctg ctcgcgtaag agtttcgaag gctgtcgatc gtcgacgata
     3961 gctcgttggc ctgctcggtc agcgcgcgct ccgacatgtt gcgcaggtcg agccagaccg
     4021 cgaccggcag tccggccagg acgagcagca cgagcatgat ggtgatggaa agcctgcgcg
     4081 cgaagaagcc gctgattcgc tcgctcaacg cggccgcgcg gccgggggcc gtcggagcat
     4141 cgtggggcgg ctctgcggga tatggcgcgg ttgcgaccat ctcggcctcg ctttcgagtg
     4201 cttcggcgct atgccatggg acggcaggta gtgtcccgat tgcggccttc gcaagccctt
     4261 gcagcacact ttcgtgcgag cgtcgcaata aaagcgagag cgcctgcgag cgggctacgc
     4321 gctccccgtc acccgccacg acgcgaatcc ggcgccgacg agcgtctgcg ggctcgggaa
     4381 atagtcgatc agctcgaagc ctttgctgcc ggccgcggcg tgggcgatca cccagtcacg
     4441 gatttcgtgc gcgccgttgc cggtgcgcgc cagcgcatcg gtgaggaacg ccgcaagccg
     4501 cgcgtcctcg ccgtcctcga agtgcttgat gcaggcgtga tcgaactctt cgtcgatcca
     4561 ccccatcgtc ggctcgccga tcgagtggct caagccgcca gtgccgagca tcgccacccg
     4621 caacggctcg gggtagctct cgatcgcttg tctgagcagc cggccccagg cgaggcagcg
     4681 ccggatgctc ggcatcggct cctcgaggtc gttgacgatc acgggcacga tcgggagctc
     4741 ggcgtcgacg ccgatgcgcc agagcgggat gcacgtgccg tggtcgagct tgaggcggtg
     4801 cgacagcgcg ggctcgaaat cgccgttgag cgcggtcttg agcagatggc gcccgaacgc
     4861 ggcgtgacct ttgaggcgct cgcggggaaa cacctttttc atgaacggct cgggcgggcc
     4921 gtcgtgctcg tcgccgatgc cgatgcagac cgccggcagg ttgctgatga agaagttgac
     4981 ccagtgatcg ggcgagatga cgacgagcag gtccggacgg cacgccttga gccgccgccc
     5041 gatctcgtcg aaggcggccg ccagccggtt gcggtggtct cgcggcatgg tctcccaatc
     5101 ccgcgcgatg atcggcccgt ggctggccgc gaacacgcca acgagatcag ccatcgacct
     5161 tccctttccc ggccttgagc gcgtgcagcc gcgcgatgaa ctcgccctcc ggcatgccgc
     5221 ggatctggaa gtagaagcgc aagaggtagg cgttgaccag cggcgcgatc ttgcccacgt
     5281 cgtcctcaag cagagccttg cgggcgtcgg gattgatctc gtagcgctcg agcacggcgg
     5341 gcatattggc gcgatactcc gccgccagct tcgaatcgtg ctgcagatcg aagaacagct
     5401 tgctcaccca gtagtcggtc attgccgctc cgggactgat gatttaagcc catgaggcac
     5461 gattgcctcg ccccgcgatc ttagcccagt cgcggtcccg ccgtcattcg tcgcccgcgc
     5521 ttacttcagc cgcttcgccg cctcttccac gatgctcagg tccgtgtgct tcttgacgtc
     5581 gggggccgat ttgacgaagc caagatcgcg cgtcatgtcg acgttcttct gcagcgccac
     5641 cagatcggga agcatgttgg ggtcgtggta ggcgtcgcgc ttggtgaaca gccaatcgaa
     5701 acgctcgggt ggctgcttgg tgacgcggcc cgcgatctcc atggcctcct tgtggttgtt
     5761 cggatcgagg taccaacgcg tgatgcgcag cgtgtcctcc atgaagtcga ccatggcggc
     5821 gcgattcttg tcgaggaacg gcttccgcgc ggtccacacg atcatgtcgg tgacgccgat
     5881 cgcatcgcgc tgcacgaaca gcgtgcggcc gatcttgcgc aactcgggat catacgagaa
     5941 cggaatgacc gcggggatca tgtccacctt cttctccgac agcatcgcgc gcatggtcgg
     6001 gaaagccgac tcgactatcg tgtagtcgcg cttgtcctcg aggccgaact tgcgcagcat
     6061 cgcgcgcatg gcaacgtcga cggcgctgcc ggccgcgttg gtcatcacca ccttcccctt
     6121 gagatcgtcg actttcttga tcgggctgtc ggcgagaacc atgtactcct gcgagtaata
     6181 gccctcgacg ccgtcgcgga actcgtccgc gatgatgcgt acgtcgtcca tgccggcgtt
     6241 ctcgatcgcg attgcaagcg tcgaataggc gaggttggag acttcgagct cgccgttggc
     6301 gatcgccgtc accatgggtg gggtgccggc gaagcggacc ggctcgagcg tgtaggactt
     6361 gccgagatgc cgcgcgaggt cctttttctc cagcatgatc gaggcccagt tggtgaccgg
     6421 ggcgacccag gatatgcgga tcttgacggg ctccgcgctc tgcgccgcgc tcgcaccgat
     6481 gagcgcggcg gcagcaacgc tcgcaaatat caatctcgat aggcgcatgc gtgtcctcct
     6541 ggtggcgggg tttggcggcg ttcggccgat tttgacgcca cgttgcagag cgccggcggt
     6601 cttggcaagg gtcgaaattg gcgcacgctc ggcgaaggcg cggcaatgga ttttgtcgcg
     6661 cgccgctacg gcgaagcttc ctccagcacg cgtcccttgg tttcgaccgc gtagaggccg
     6721 gtgatcacgg ctgcgacggc cgcgacggtc gcgaacgtgg cgaacaccga gggcaggccg
     6781 ccggcgagca tcattccaac cacgttcggt ccgatgatcg aggcaaaccg cagccaggcc
     6841 gtggcggtcc ccaccgcgat cgcgcgtacg cgggtggggt agagttcggg ggtgtagaga
     6901 tagacgccga tattgatggt gctgatgaag aaataggcga tggtcataaa ggtcagcacc
     6961 tgctccgcgc tgggagcaga gaagagcgca agcgtggcca aggccagagt cgcagccgcg
     7021 aaggagaagg caaaccacgc ccgccgcccc acatggtcga tggtcaatgc gcagatcagg
     7081 gtgccgagaa ggccgacggc ctgcgtgatc aatccgtatc gcagcgacac gtcgagcggg
     7141 agcttgaata cggttcgata caacgtcggc agccagatcg agagcccgta gttcacgaaa
     7201 taggccgaga ac
//
LOCUS       DATLJT010000299         1733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2084303,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000299 DATLJT010000000
VERSION     DATLJT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084303"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VM910_06820"
     CDS             <1..237
                     /locus_tag="VM910_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HVI62290.1"
                     /translation="PSEMMYRRALDHFTCLYEESGERAKIMAIACHPYLSGVPHRIGH
                     VQRTFEEILSRDGVVAWDGSKILDWYLAQQGQRG"
     gene            248..1444
                     /locus_tag="VM910_06825"
     CDS             248..1444
                     /locus_tag="VM910_06825"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI62291.1"
                     /translation="MSVARSLSAFVPHDLTAPLSGAGAGPLAGLTAAVKDMYDIAGYR
                     TGGGSPDWLDAQAAATSTAAAVVRILEAGATIVGKTVCDEFFYSVSGANAHYGTPINP
                     RAPDRLPGGSSSGSASATAAGTCDFALGSDTGGSVRVPAAFCGIYGIRPTHGRIDLSG
                     AMPMAPSFDVAGWFASGPGIFRRVGEVILDRSRTPAPIENLTVLEDAFEQADKDVAIL
                     LREALDDMTDALPKARSGRVASDGLDPWRETFRVIQAGEVWRNYGEFVKTHRPRLGPG
                     IRERMEFAAKVPERDIEQARQEHARARDHIRAMAQPGTILALPTAPCVAPLLDTPSDA
                     LESFRVRVMRLTCIAGLGGLPQVTLPIGSLAGCPVGLSFIGWAGGDEALLALAVKLAR
                     HRRYAN"
     gene            complement(1461..>1733)
                     /locus_tag="VM910_06830"
     CDS             complement(1461..>1733)
                     /locus_tag="VM910_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI62292.1"
                     /translation="TRTLAFEEVAHGVRVNAVCPGSTLTDFHISKARAAGKSVETLKT
                     ERQTTSLLGRWATPQEIAWPILWLASDEASFITGATLAVDGGLSIM"
BASE COUNT          296 a          557 c          563 g          317 t
ORIGIN      
        1 ccatccgaga tgatgtatcg gcgcgcactg gaccacttca cgtgcctcta cgaggagtca
       61 ggcgagcgcg caaaaatcat ggctatcgcg tgccacccct atctttccgg cgtgccgcac
      121 cggattgggc atgtccagcg caccttcgaa gagattctaa gtcgagacgg cgtggtcgct
      181 tgggatggtt ccaagattct cgattggtat ctcgctcagc aaggccagcg cggataggct
      241 ggtgggcatg tcggtcgcac gatcgctctc cgctttcgtt ccccatgatt tgaccgcacc
      301 cctgagtggg gccggcgctg gcccgctggc cggtctgact gcggccgtca aggacatgta
      361 tgatatcgcg ggatatcgca ccggtggggg cagcccggat tggctcgatg cacaggctgc
      421 ggccacctcg accgccgccg cagtggttcg catcctggag gcgggcgcaa caattgtcgg
      481 caagacggtc tgcgacgaat tcttttacag cgtgtccggt gccaacgccc actacggcac
      541 cccgatcaac cctcgcgcgc cggatcggtt gccggggggt tcgtcgagcg gctcggcatc
      601 ggcgaccgct gcgggaacgt gcgacttcgc cttgggcagt gacaccggcg gatcggtgcg
      661 cgtgcccgcc gcgttctgtg ggatctacgg gatcaggccg acccatggac ggatcgatct
      721 ttccggcgca atgcccatgg cgccatcgtt cgatgtcgcc gggtggtttg caagcgggcc
      781 cggaatcttc cgacgcgtcg gcgaggtcat tctcgatcgc tcacgaacgc cggccccgat
      841 cgaaaacctg accgttttgg aggatgcatt cgagcaagcg gataaagatg tcgcgatctt
      901 gctacgggag gctctcgacg acatgaccga tgccctgcca aaggcccgaa gcggccgtgt
      961 ggcttccgat ggtcttgacc cgtggcgcga gaccttccgc gtgatccaag caggcgaggt
     1021 ctggcgcaac tacggagaat tcgtcaagac gcaccggccg cggctcggtc cggggattcg
     1081 cgaacgcatg gaattcgcgg caaaggtgcc ggaacgggat atcgagcagg cacggcagga
     1141 gcacgcgcgg gcgcgcgatc acatccgcgc aatggcgcaa ccgggcacca tccttgcgct
     1201 gccaaccgcg ccttgcgtcg cgccgctctt ggatactccg tcagatgcgc tggaatcgtt
     1261 ccgcgtgcgc gtcatgcgcc tcacttgcat cgcgggcctt ggcggactgc cccaggtcac
     1321 tctgcccatc ggatcgcttg ccggctgtcc cgtcggactc tcgttcatag gatgggccgg
     1381 cggcgacgaa gccctgctgg cgctcgccgt gaaactcgcg cgccaccgcc gatacgcaaa
     1441 ttaagcttaa aacagcgccg tcacataata gaaagaccgc catcgacggc gagcgtcgcc
     1501 ccggtgatga acgaggcttc gtcgctggcc agccagagaa tcggccaagc aatctcctgc
     1561 ggcgtcgccc agcgcccgag cagcgatgtc gtctggcgct cggtcttgag cgtctcgacg
     1621 ctcttgccgg cggccctggc tttcgagata tggaagtcgg tcagcgtcga gccgggacag
     1681 accgcgttga cgcgtacgcc gtgcgccact tcctcgaagg cgagcgtgcg cgt
//
LOCUS       DATLJT010000300         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2774011,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000300 DATLJT010000000
VERSION     DATLJT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774011"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VM910_06835"
     CDS             complement(<1..490)
                     /locus_tag="VM910_06835"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62293.1"
                     /translation="MRRREFITLLGGAAAAWPLAAHAQQTAMPVIGFLRDATAAGSEF
                     MVNGLRKGLAEAGFVEGHNLTIDYAWSEGQSVRLSTLAAELVGRNVSVVVSSAINATF
                     AAKAATSTIPVVFAINIDPVASGLVASLNRPGGNLTGVSYLTSELGAKRLGLMHEMVP
                     RVT"
     gene            complement(487..1482)
                     /locus_tag="VM910_06840"
     CDS             complement(487..1482)
                     /locus_tag="VM910_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62294.1"
                     /translation="MSGMRRREFITFLGGAAAAWPLAARAQQSPAMPVIGFLSSRSPE
                     DSAHLIEAFRRGLSEGGFVAGQNVAIEFRWAHGQFDLLPAMASELVGRRVSVFVAVGG
                     EPSALAAKRATSTIPIVFAIGSDPVSAGLVESFNRPNGNLTGITLQTVFMEPKRLGLL
                     RDLVPRALLIGVLLNSSFALAASQRQEIEEAARGIGQRIVIVDASTDGELDAAFAELA
                     KQRADALLVAASPYFDTRRDHIVLLAQRHRLPAIYQFREFAMAGGLLSYGIAITEGYR
                     QVGIYSANILRGAKPADLPVQAISKFELLINLKTAKALDIKISDNLLSLADEVIE"
     gene            complement(2348..>2690)
                     /locus_tag="VM910_06845"
     CDS             complement(2348..>2690)
                     /locus_tag="VM910_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62295.1"
                     /translation="IEYMKAGKVRPLAITTTTRSEALPDVPTVSEFVPGYEGSFWFGV
                     GAPKTTPVEIVDKLNKEVNAALDDPKMKARLADLGGVALPGSPAEFGKLIADETEKWA
                     KVIKFAGIKAD"
BASE COUNT          503 a          869 c          794 g          524 t
ORIGIN      
        1 cggtaaccct tggcaccatc tcgtgcatca gccccagtcg ctttgctcca agctcggagg
       61 tcaagtagct cacaccggtg agattgccgc ccgggcggtt gaggctcgcg accagcccgg
      121 atgccacggg atcgatgttg atcgcgaata cgacggggat cgtggaggtc gcggccttgg
      181 ccgcgaacgt cgcgttgatc gcactgctga cgacgacgga cacgttccgc cctaccaatt
      241 ccgccgcgag cgtcgataac cgcacgctct ggccctcgct ccaggcatag tcgatggtga
      301 gattgtgacc ctcgacgaaa cctgcttcgg cgagtccctt gcgcagccca ttgaccatga
      361 actcggaccc cgctgcggtg gcgtctcgga gaaacccgat cactggcatc gccgtctgct
      421 gcgcgtgcgc cgcaagcggc cacgctgccg cagcgccgcc cagcagcgtg atgaactcgc
      481 ggcgcctcac tcgatcacct cgtcggcgag cgagagcaga ttgtcggaga ttttgatgtc
      541 cagcgctttt gcggtcttga ggttgatcag cagctcaaat ttgctgatgg cttgcaccgg
      601 caagtccgcg ggctttgccc cgcgaagaat gtttgcgctg taaatcccga cttggcgata
      661 cccctcggtg attgctatcc cgtaactaag caatccgcct gccatggcaa attcacgaaa
      721 ctgatagatc gcgggcagcc ggtggcgctg cgcaagaagg acaatgtggt cgcgccgagt
      781 gtcgaaatac ggcgaagccg ctaccaacag cgcatcggcc cgctgtttcg ccaactcggc
      841 gaatgcggcg tccagctccc cgtcggtgct ggcatcgaca ataacaatgc gttgaccgat
      901 gccgcgcgcc gcctcctcga tctcttgtcg ttgggaggcg gccaacgcaa agctcgaatt
      961 gagcagaaca ccgatcagca acgctcgcgg caccagatcg cgcaacagac ccaaccgctt
     1021 cggttccata aaaaccgttt gcaaggtgat tccggtgaga ttgccgttgg ggcgattgaa
     1081 gctctcgacc aagccggcac tgaccggatc gctgccgatc gcaaacacaa tcggaatggt
     1141 cgacgtggcg cgcttggctg ccagcgccga tggctcgcca ccgacggcga caaacacact
     1201 cacgcggcgg cccaccagct ccgaggccat cgcgggcaaa agatcaaatt gaccgtgtgc
     1261 ccagcggaac tcgattgcaa cattctggcc cgcgacgaag ccgccctcgc tcaggccccg
     1321 ccgaaacgcc tctatgagat gcgcggaatc ctccggcgag cgcgaactca aaaaacctat
     1381 caccggcatc gccggcgact gctgcgcgcg cgccgcgagc ggccacgccg ccgcagcgcc
     1441 gccgagaaac gtgatgaact cgcgccgtct catgccggac atggggcttc ctcccccgtc
     1501 ccggagtaat caccggcgac tgccgactcc tggggtcact cgacccgcac gcttagcctg
     1561 ccacgggaaa ctgccctctg ggtagctttc gagcatcgcc tcattgaggg ggctgacatc
     1621 agcagagccg tgcatgaacc caacagcagg cactgctgcg gcaaagtaaa ataggctcgc
     1681 gcgtcggtcc atgaatggga gcgaacccgg cgagtaggca tgatcagcga ccatgttctg
     1741 tcccccttcg gctcgaaaac ggcacgattg tttagggtct aagccgtcta cgttgcactg
     1801 tgccgccagg tgggagcgaa gtccttgggg caaaccttaa ttgttctgaa tcttgctagg
     1861 catccggtgg tggggagatc gctgcactgc gggattcagg cccagctatg tccgcctggt
     1921 tatgcgatcc gcgccttgcc ttgtgccttt ggatagtatc gggacaccta gacttccctc
     1981 caggtgttct ttcctgcctg aagccccgga acctgtgccc ggggctttct ttgtctctct
     2041 gtgtgtatca gcgggaacga accggcggcg gggccgctct gatgcgcatt aatctttgga
     2101 ttgtcctcgt tgtcctaatg gcgctcttgg cggtcactca gctcttgcga tatcggcaag
     2161 atacgcaacg catctgcgcc gatgacccga gcgcctcggt gtgcgggcgg tgagcttcgc
     2221 gcttcacgca gtcccccagc caccatccct ggacgctcgc cacgcacatt atgagttagg
     2281 acacagagcg gtcatccggg acgcattcgc ggaacgggat ttagaatatt ggatcgggac
     2341 tgccgcgtca gtccgccttg atgcccgcga acttgatcac cttggcccac ttttcggttt
     2401 cgtcggcgat cagctttccg aactcagccg gcgatcccgg cagcgccacg ccgccgagat
     2461 ccgcgagacg ggccttcatc ttcgggtcgt cgagagccgc gttgacctcc ttgttgagct
     2521 tgtcgacgat ctcgaccggc gtggtcttcg gtgcgccgac gccgaaccag aaactccctt
     2581 cgtagcccgg aacaaactca ctgacagtcg gcacgtcggg taacgcctcc gagcgcgttg
     2641 tggtggttat tgccagcggg cgcaccttgc cggccttcat gtactcgatg
//
LOCUS       DATLJT010000301         5719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_935735,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000301 DATLJT010000000
VERSION     DATLJT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935735"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VM910_06850"
     CDS             <1..240
                     /locus_tag="VM910_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HVI62296.1"
                     /translation="DTLNRSLRGWSNYFCYGTRRSAFRSVDRYTYERVRDFLARRHKV
                     AGRSTRRFSYDTVYRELGLLRLERLPLAAASCASR"
     gene            complement(932..2248)
                     /locus_tag="VM910_06855"
     CDS             complement(932..2248)
                     /locus_tag="VM910_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVI62297.1"
                     /translation="MRPRLDLRKLRVVGALLLAWVPCLAQAQAPLDANATSSAIAGNP
                     GAVNIAPGTGELGRLLGFEPESGLRLGGVLVSNGNYLISGGNEPKTTSFNNLLLTDFE
                     ADLDQLAHIPGASFGTAMLRFDGQVTNQQAGIVTGYNSLTGVPPLHRTELYELWWRQS
                     FFADGLVVRIGKTVPTYDFGNVIRPVPVQDLSLQIPAVSGLLYTPVFVNPTILGALPG
                     YYNSAYGVTATVAPTQRFYLSLGAYDGNGARGVQTGLQATPTFNGYRFQIAETGTAWL
                     LGPDNLPGAFAIGGWDQTGTLTASTPQGPITQNGTHGVYAFASQRLWRGSGEDDARGV
                     SGFVQLGANNSRTMIAARYVGLGLTAFGVIPGRPTDSLGAGVAWSGLNGNRNLRPNET
                     LLQLYDQIQVFGSFYLQPTLTFSPNPGEKTAHAPAVALTLQTTILF"
     gene            complement(2279..3247)
                     /locus_tag="VM910_06860"
     CDS             complement(2279..3247)
                     /locus_tag="VM910_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI62298.1"
                     /translation="MSDNVSTADKAVTLVTQTRVREGMAEEFGCWQSTISAAAAEFPG
                     FITQSVLPPNPPLQTDWVIQQRFTNVGAASAWLRSERRMRLLDAGQTMLAGPDDVHLV
                     RDVAGWPRPAPVSAVIASRVRPGQEAAFRAWEQRIATAQAQSPGFQGYRFEPPIPGVQ
                     DDWLAILRFDSERNLQTWLDSAERKRLLEEAAPFLEGFHARVVRTGFEQWFPSAEDGA
                     SAPAAWKQNMIVLLLLYPVVFLFGIWVQTPVLMGRAKLPFWFALFIGNIVSVVLLNWL
                     VPLASGGFGWWLSPAHDATSRRISLAGAALVIVLYAACLFLFSNMA"
     gene            complement(3244..5349)
                     /locus_tag="VM910_06865"
     CDS             complement(3244..5349)
                     /locus_tag="VM910_06865"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62299.1"
                     /translation="MEQARATSVPGVVAIFTWEDVPRRLYSTATHEDHLVDPDDTYVL
                     DNVVRFVGQRVAAVVAETEAAAEAACRLIDVRYEILPAVFDPEAAMQPEAPLLHDKGI
                     AAYGNVYVDIHGEVGNTAEGFAAADVVHEGVYSTSRVQHAHLETHGSIAWTAPDGRVH
                     VRTSSQAPFIAQQKLCHIFGLRARDMHVFTERVGGGFGGKQEMLTEDLCVLAALKTAR
                     PVKWEFTREEQFIGATTRHQMTAKVRLGARCDGTLTAIEVRVVSNTGAYGGHGGETLA
                     AALAGPLAAYRCPNKKADGYAVYTNLVPAGGFRGYGSSQTTFAIECAIDDLAKKLALD
                     PSEIRRRNMIRPDDWIESIWGEPSDVDFGSYGLDQCMDLVERALKSGRGEPKPKGEDW
                     VQGTGSALAVLECGPPTEHRSGAVMRLLADGRYHLAVGSTEMGNGSVTSHRQIAAEVL
                     GTRAGQIDIVNADTDLTPYDTGTFASTGTVVAGQAVAVTAAALRDNILDYASRHSGVP
                     LTECRLADDAVICGSCRVPLSKLFAAGTSERHRFEARRKSYLSPRSIAFNVHGVRVAV
                     NCVTAEIRILHSAHAADIGRLINPMQCRGQIDGAIAMGFGWALTENMVHDTNGAIVNP
                     SLRNYRIPAFADVPASEVFFADTYDRIGPLGAKSQGECAINPVAPAIANAVANATGIR
                     FAQLPLTPDRIFAALSAQP"
     gene            complement(5231..5668)
                     /locus_tag="VM910_06870"
     CDS             complement(5231..5668)
                     /locus_tag="VM910_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI62300.1"
                     /translation="MGAQAFQCGFCTAGMIMTAAALNPDQRTDLAHALKGNLCRCTGY
                     RAIADALAGVQSVEEDVAGQACGASLRNPFAEAIVTGRARYTMDFAMEGLLAIAPCAR
                     ADSPDRASARHFCTGRRRHFHLGRCAAPPLQHCHSRGSSRRSR"
BASE COUNT         1105 a         1798 c         1780 g         1036 t
ORIGIN      
        1 gacacgctaa acagatctct gcgcggctgg tcgaactact tttgttacgg gacgcgccgg
       61 tcagcattcc gcagtgttga ccgctatacc tacgagcgcg tgcgcgactt tctcgcaaga
      121 cggcacaaag tggcagggcg cagcacccgc cggttctctt acgacactgt ctatagggaa
      181 ctcggtctgc tgcgcctcga acgcctgcca ctcgctgccg cgtcgtgtgc ctcgcggtga
      241 agccagtcgg agagccggac tccggaaatc gggacctccg gttcgatgag cggagatggg
      301 aaacggggtg atggccatag gcccccagct accgcgccca tcttcgactc tacccgacga
      361 gacgattgct tgagctggcg ggttggtcgg catgctgggg tgaagctgac gctcgctaac
      421 aactgcagga tctctgcagt tgaccgttgg agacatccag cggaacgcaa gcccccacga
      481 ttgctgctca aggatagtac caagggtatc tgcactgccg ctagggcacg ggtcggcggg
      541 caccctccac gcggccggac caccgtcttt gcgcggtccg ccgagccgtt ggcggagtgt
      601 cccgggtcga gtcgctgcga atgctgcggg atttgtcaca atgctgaagc atgctttctc
      661 gtcgcctgta accggctcgc gcaactactc aacgttctcc tggcgaaagc gcaacgtgca
      721 gtgcagacca gcagccagca catggtgcgg gttgggcaaa tcgaggcgga tgccgaaagt
      781 gccaccgccg gcgtcgaaca cgcgatcgac aatgcttccc gtaccgtata actgccgccg
      841 atcggctccg cagacttggc gcgtacgctc cgttctgccg tgccttaaac cggtatcaag
      901 gggctatagg aacggttcaa acgcgccgcc attagaagag aatcgtagtc tgcagcgtga
      961 gcgcgacggc gggagcgtgt gcggtttttt ccccggggtt gggtgagaaa gttagcgtgg
     1021 gttgcagata gaagctgcca aagacctgaa tctggtcata gagctgcaat agggtttcgt
     1081 tcggccggag attgcgattg ccattcaggc ctgaccacgc aacgccggcg ccgagcgaat
     1141 ccgtcggccg gcccggaata accccgaatg cggtcaggcc gagccccaca tatcgggccg
     1201 cgatcattgt ccgcgagttg ttggcgccca attgcacaaa cccagatacg ccgcgcgcat
     1261 cgtcttcccc gctcccgcgc caaagccgct gcgatgcgaa cgcgtaaacg ccgtgggtgc
     1321 cgttctgtgt gattggacct tgcggcgtcg acgcggtgag cgtccccgtt tgatcccaac
     1381 cgccgatggc gaacgcaccc ggcagattgt ccggccccaa cagccaggca gtgccggtct
     1441 cggcgatttg gaaccggtac ccgttgaagg tcggcgtcgc ttgcaggccg gtctgcacac
     1501 cccgtgcacc attgccgtca taggcgccaa gagagaggta gaagcgttgg gtcggcgcga
     1561 ccgtcgcggt gactccataa gccgagttgt aatagcccgg gagcgcgccg agaatggtcg
     1621 ggttgacaaa caccggtgtg tagagaagac cgctcaccgc cggaatttgc agcgacaagt
     1681 cctgcaccgg aaccgggcgg atcacgttgc cgaaatcata ggtcggaacg gtctttccga
     1741 tccgcaccac cagtccatcc gcaaaaaagg attgccgcca ccagagctcg tagagctccg
     1801 tccggtgtag tggcggcaca ccagtcaggc tgttgtagcc ggtcacgata ccggcctgct
     1861 gattggtcac ttgtccgtca aagcgaagca ttgccgtacc aaagctggcg cccggaatat
     1921 gcgcgagctg gtccaaatct gcttcgaaat ccgttagtag caaattgtta aagctggtcg
     1981 tctttggttc attaccaccg gagatcagat agttgccgtt gctgactagc accccgccta
     2041 agcggagacc gctttccggt tcgaagccga gcagccgtcc cagctcgccg gttcctggcg
     2101 cgatattgac tgcgccggga tttcctgcga tcgccgaact ggtcgcgttc gcgtcgagcg
     2161 gggcttgagc ctgcgccaag cagggaaccc aggccaggag cagagccccc accacccgca
     2221 gtttgcggag atcgaggcgc ggacgcaccc gaggtttccc aggaggggcg tcctttccct
     2281 acgccatatt cgagaacagg aagaggcagg ccgcgtaaag aacgatcacc aatgctgcac
     2341 ctgcgagact gatacgcctg ctcgtcgcat cgtgcgccgg cgagagccac caaccaaaac
     2401 ctccactcgc caatggcact agccagttga gcaataccac gctgacgata ttgccgatga
     2461 acagggcgaa ccagaacggc agcttggccc ttcccatcag gactggtgtt tgcacccaaa
     2521 tgccgaacag aaatacaacc ggatagagta gaagcagaac gatcatattc tgtttccagg
     2581 cagccggtgc cgaagccccg tcctctgccg acgggaacca ctgttcgaag ccggtacgca
     2641 ccacacgtgc atgaaacccc tcgaggaagg gtgccgcttc ctccagcaac cttttccgct
     2701 ccgccgaatc aagccaagtc tggagattgc gctcgctatc gaagcggagg attgccagcc
     2761 aatcgtcctg cacgccaggt atcggcggct cgaaccggta gccttgaaag ccgggtgatt
     2821 gtgcctgggc cgtcgcgatg cgctgctccc aagcgcgaaa cgctgcttcc tggcccggtc
     2881 tgacgcggga ggcaattacc gcagagaccg gtgccggccg cggccagccg gcaacgtcgc
     2941 ggaccagatg cacatcgtcc ggcccagcca gcatcgtttg cccggcatcc aggagtcgca
     3001 tccgccgctc ggaacgaagc caggccgacg cggctccaac attggtaaag cgctgctgaa
     3061 tcacccagtc agtctgcagg ggcggattgg gcggcaacac gctttgggtg ataaacccgg
     3121 gaaattctgc cgccgcagca ctgatcgtac tttgccaaca gccaaattcc tccgccatgc
     3181 cttcgcgcac acgggtctgg gtgacgaggg tcaccgcctt gtcggctgtc gaaacgttgt
     3241 cgctcacggt tgtgccgaaa gtgcggcgaa gatgcgatcc ggcgtcagcg gcagttgcgc
     3301 aaagcggatg ccggtggcat tggcaacggc gttcgcaatc gctggtgcca ccgggttgat
     3361 ggcacactcg ccttgcgatt tggcaccgag agggccgata cggtcgtatg tgtcagcgaa
     3421 gaatacctcg ctcgctggca catcggcaaa ggcaggaatc cggtagttgc gcaggctggg
     3481 gttcacgatc gccccattgg tgtcgtgcac catgttctcg gtcagtgccc aaccgaagcc
     3541 catcgcgatc gccccatcga tctgaccacg gcattgcatt ggattgatca gccggccgat
     3601 gtccgccgca tgggcgctgt gcaggatgcg gatctcggcg gtgacgcagt tcacggcgac
     3661 gcgaacacca tgtacgttga acgcgatcga ccgcggggaa aggtaggatt tgcggcgcgc
     3721 ttcgaagcga tgccgctcgc ttgtgccggc ggcaaacagc ttcgagagcg ggacccggca
     3781 cgagccgcag atcaccgcgt catcggcaag ccggcactcc gtcaagggaa cccccgagtg
     3841 tcggctggca tagtccaaaa tgttgtcgcg cagcgccgcc gccgtcactg ctaccgcttg
     3901 gccggcgact accgtcccgg tgctcgcgaa ggtaccggtg tcatacggcg tcaggtcggt
     3961 gtcggcattc acgatgtcga tctgcccggc ccgcgtgccc aaaacctcgg cggcgatctg
     4021 ccggtgcgag gtgacggagc cattgcccat ttccgtcgat ccgacggcca agtgatagcg
     4081 tccgtctgct agcagccgca tcacggcgcc ggatcggtgc tcggtcgggg gcccgcattc
     4141 cagcacggca agcgcagaac ccgttccttg cacccagtct tcacctttgg gcttgggctc
     4201 gccgcgcccg ctcttcagcg cgcgctcgac gaggtccatg cactggtcga gtccgtagct
     4261 gccgaaatcc acgtccgacg gttcgcccca aattgattcg atccagtcat cgggacgaat
     4321 catgttacgg cgccggatct cggacgggtc gagcgcaagc ttttttgcca gatcatcgat
     4381 cgcgcactcg atagcgaagg tggtttgcga ggagccgtag ccgcggaagc cgccagcggg
     4441 caccaagttg gtatagaccg catagccatc ggcctttttg ttcggacagc gataggccgc
     4501 gagcgggcca gccagcgccg ctgccaaggt ttcgccgccg tgacccccat aggcgccggt
     4561 gttggagacg accctgacct cgatcgcggt gagcgtgccg tcgcatcggg cgccgagccg
     4621 caccttggca gtcatctggt ggcgtgttgt ggcgccaata aactgctcct cacgcgtgaa
     4681 ttcccatttc accggccgcg cagtctttag cgcggcgagc acgcaaagat cctcggtcag
     4741 catctcctgc ttgccgccga acccacctcc gacccgttcg gtgaacacgt gcatgtcccg
     4801 cgcgcgcaaa ccaaagatat gacagagctt ttgctgggcg atgaacggcg cctgcgagct
     4861 ggtgcggaca tgaacgcgtc catccggcgc ggtccacgcg atcgagccgt gggtttcgag
     4921 atgcgcatgt tgcacgcggg aggtggaata gacgccctca tgcacaacat cggcggcggc
     4981 gaaaccttcc gccgtgttgc cgacctcgcc gtgaatgtcg acatagacat tgccgtaggc
     5041 agcgatgccc ttgtcgtgca acagcggtgc ttcgggctgc atggccgcct ccgggtcgaa
     5101 caccgccggc aagatttcgt atcggacgtc gatgagccgg cacgcggcct ccgccgctgc
     5161 ttcggtttcc gcgacaaccg cggcgacgcg ctgcccgaca aagcgcacca cattgtcgag
     5221 gacgtaggtg tcatcgggat cgacgagatg atcctcgtga gtggcagtgc tgtagaggcg
     5281 gcgcggcaca tcttcccaag tgaaaatggc gacgacgccc ggtacagaag tggcgcgcgc
     5341 ttgctcgatc tggagaatcc gcgcgtgcgc atggggcgat cgctagcaga ccctccatgg
     5401 cgaagtccat ggtgtaacgg gctcgcccgg tgacgatcgc ctcggcgaag gggtttcgca
     5461 ggctcgcccc acaggcctga ccggcaacgt cctcctcaac agactgcacg cccgcaagcg
     5521 catcggcgat cgcacggtat ccggtacagc ggcaaagatt gcccttgaga gcatgagcaa
     5581 gatcggtacg ctgatcggga ttcagtgcgg ctgcagtcat gatcatgccg gccgtgcaga
     5641 agccgcactg gaatgcctgg gcacccataa aggcctgctg caccgggtgc aggtggccgt
     5701 tctccgccaa gccttcgat
//
LOCUS       DATLJT010000302         3812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3922256,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000302 DATLJT010000000
VERSION     DATLJT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922256"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..88)
                     /locus_tag="VM910_06875"
     CDS             complement(<1..88)
                     /locus_tag="VM910_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVI62301.1"
                     /translation="MAIADKLVLQRQRHLKWRALDRLELILMV"
     gene            complement(125..1126)
                     /locus_tag="VM910_06880"
     CDS             complement(125..1126)
                     /locus_tag="VM910_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62302.1"
                     /translation="MNFPAKILGGLLAGAALIFAATANAQEVKHYRFAHDQQLNSGYS
                     VAYDIFSAKLKELSKGTMLVDQYPGAQLGQEPQILQLVKAGDIDFAVVSSANTSTISP
                     QAGVMSLHFLFRNENHIVKSLGDEKVYAAIRDMIDDTTQGIHAIGLATQGFRYMYGKK
                     EVHKVDDVKGYKVRVQATATEDTMFPAYGAQTVHMPFGSVYTSLQTGVVDFAENGMNV
                     YLINKHYEVAPVLSMTEHEANNAILWISDKLWQSLSAEQKQWVTTAARDVSAQEPKRG
                     FELEHAAAAKLEKMGVKIVTDVDKPGFQKIADPYLDKLAHDLGPHAEKIKNLIRGVN"
     gene            complement(1275..2273)
                     /locus_tag="VM910_06885"
     CDS             complement(1275..2273)
                     /locus_tag="VM910_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62303.1"
                     /translation="MASLSRVLGAVVAACLFAGANATAQEVKHYRFAYDQPKTTGYGI
                     LGDIFADKLKELSKGTMLIDQYPGAQLGQEPQVLQLVKNGDVEFCISSSANAATLSPQ
                     AGVMSMHFLFRSEGHLIKAMADPEVSKAVKAMIADTVQGARVIGLATLGLRSMYSKRE
                     IKNIAEIKGLKVRVQATPTEDTTFPAYGAQIVHMPFGSVYTSLQTGVVDVAENGVNVY
                     LANKHYEVAPVLSMTEHEANNSLVWISDKLWNSLTPEQQGWVQAAADEVNKTQPAKAI
                     ELEHQSQDKLRSIGVKIVTDVDKSGFIAVADPLLDKLAKELGPHAEKIKNLIRGIN"
     gene            complement(2352..>3812)
                     /locus_tag="VM910_06890"
     CDS             complement(2352..>3812)
                     /locus_tag="VM910_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVI62304.1"
                     /translation="IAESFAYPTSMYLRNLMAMDTEEMIRAQPMDAVIVIGGCDKTLP
                     AQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGACTDCRRLWAKYRAGEIDDKEIEAVN
                     GRLAPSVGTCMVMGTASTMACITETLGLSLPMSATIPAPHAERFRLAEASGKVAAAMA
                     KAKGPKPSEVLTKSSFRNAQVVLQAIGGSTNGLIHLTAIANRTPHGIDLGAFDKLGRE
                     VPVLVDLKPSGDHYMEHFHHAGGVPKLMAQLGDLLDLDAKTIEGKTLREVVVAAEDVP
                     GQTAIRPRQDPIKPEGGLAVLHGNLAPRGAVIKHSAATAKLLQHTGRAVVFESVEDMT
                     LRVDDPALDVTADDVLVLRNAGPKGAPGMPEAGYLPIPKKILRTGVKDMVRISDARMS
                     GTAFGTIVLHITPESAVGGPLALVKTGDMIRLDVARRSIELLVDEAELKKRQAALKPV
                     GTAEWAGRGYAHLFNETILQADEGCDFDFMRGKGKG"
BASE COUNT          633 a         1213 c         1158 g          808 t
ORIGIN      
        1 gcaccatcag gatcaattcg agcctgtcga gcgcccgcca cttcagatgg cgctgccgct
       61 gcaaaaccag tttgtcggca atcgccatcc tttcccccgt cgcggaagcg gcggcatgtg
      121 cggtctagtt gaccccgcgg atcaggttct tgatcttctc ggcgtgcggg ccgaggtcgt
      181 gggcgagttt gtcgagatag ggatcggcga tcttctggaa gccgggtttg tcgacgtcgg
      241 tgacgatctt cactcccatc ttctcgagct tggctgccgc cgcgtgttcc agctcgaagc
      301 cgcgcttggg ctcctgagcg ctgacgtccc gcgccgccgt ggtgacccac tgcttctgct
      361 cggcggagag gctttgccag agcttgtcgc tgatccagag aatggcgttg ttggcctcat
      421 gttcggtcat cgacagcacg ggcgcgactt cataatgctt gttgatgaga tagacgttca
      481 ttccgttctc ggcaaaatcc accactcccg tctgtaacga ggtgtagacg ctgccgaacg
      541 gcatgtgcac ggtttgtgcg ccgtaggccg ggaacatggt gtcctcggtc gcggtggcct
      601 gcacgcgcac cttgtagccc tttacatcgt cgaccttgtg cacctccttc ttgccgtaca
      661 tgtaccggaa gccctgggtg gcgagcccga tcgcgtgaat gccctgcgtg gtgtcatcga
      721 tcatgtcgcg gatcgcggca tagaccttct cgtcgccgag cgacttgacg atgtggttct
      781 cgttgcggaa caggaaatgc agcgacatca cgccggcttg cggcgagatc gtcgaggtat
      841 tggctgacga gacgacggca aaatcgatat cgccggcctt cacaagctgc aggatctgcg
      901 gctcctgccc gagctgcgcg ccgggatact ggtcgaccag catggtaccc ttgctcaact
      961 ccttcagctt tgcggagaag atgtcgtagg cgacgctgta accggaattg agctgctgat
     1021 cgtgggcgaa gcgataatgc tttacctcct gcgcgttcgc ggttgcggca aagatcagcg
     1081 ccgcacctgc cagcaggcca ccaaggattt tcgctggaaa attcaccgtt tccccctgtt
     1141 ttggtttgcg cggatcaaac catcgcattt gggagattgt caattgaaca tcggtcgatc
     1201 gcgatttgtc atgggtcctt gcgccgcgcc catggccttc ggcagagcga gctgcggctt
     1261 gttcgggcag ccgatcagtt gatgccccgg atcaggtttt tgatcttttc ggcatgtggc
     1321 ccgagctcct tggcgagctt gtccagcaag gggtcggcca ccgcgatgaa accggacttg
     1381 tcgacgtccg tgacgatctt taccccgatc gatctcagct tgtcctgcga ctgatgctcg
     1441 agctcgatcg cctttgccgg ctgcgtcttg ttgacctcgt cggctgccgc ctgcacccag
     1501 ccctgctgct ccggcgtcag gctgttccag agtttgtcgc tgatccacac caggctgttg
     1561 ttggcctcat gctcggtcat cgacagcacc ggcgccacct cgtagtgctt gttggcgaga
     1621 tagacattga cgccattttc cgcgacatcg accacgccgg tctgcagcga cgtgtagacg
     1681 ctgccgaacg gcatgtgcac gatctgcgcg ccgtaggcag ggaacgtggt gtcctcggtc
     1741 ggggtcgcct gcacgcgcac cttcaatccc ttgatttcgg cgatgttctt gatctcgcgc
     1801 ttggaataca tgctgcgcag accgagcgtg gcaaggccga tcacgcgcgc gccctgcacg
     1861 gtatcggcga tcatcgcctt tactgccttg gagacttcag gatcggccat cgccttgatc
     1921 agatgcccct ccgaacgaaa caggaagtgc atcgacatca cgccggcctg cggcgacagc
     1981 gtcgcggcat tggccgagga agaaatgcag aactcgacgt cgccgttctt gaccagttgc
     2041 aggacctgcg gctcctggcc gagctgcgcg cccggatact ggtcgatgag catggtgccc
     2101 ttgctcaatt ccttgagctt gtcggcaaag atatcgccga ggatgccgta accggtcgtc
     2161 ttcggctgat cgtaggcgaa gcgataatgc ttcacctcct gcgcggtcgc gtttgcgccc
     2221 gcaaaaaggc atgcggcaac gaccgcaccg agaaccctgc tcaagctcgc catcgcttcc
     2281 cccgctcatt ttgcttcgat catcccatcg gctccgtcgg gatgtcaatc aagctgaggg
     2341 gtacgcgatg gctatccttt gcccttgcct cgcatgaaat cgaagtcgca gccctcgtcg
     2401 gcctgcagga tggtctcgtt gaagaggtgc gcgtagccgc ggccggccca ttccgctgtc
     2461 cccacgggtt tcagtgcggc ctggcgcttt ttcagctcgg cttcatcgac cagaagctcg
     2521 atgctgcgcc tggcgacgtc gagccggatc atgtcgccgg ttttcaccag cgccagcggc
     2581 ccgccgactg cggattccgg cgtgatgtgc agcacgatcg tgccgaacgc cgtgccgctc
     2641 atccgcgcgt ccgaaatccg caccatgtcc ttgacgcccg tgcgcaggat cttcttcggg
     2701 atcggcagat agcccgcttc cggcatgccc ggcgcgccct tcggtccggc attgcgcaac
     2761 acgagcacgt cgtcggcggt gacgtcgagg gctggatcgt cgacccgcag cgtcatgtcc
     2821 tcgaccgact cgaagacaac agcgcggccg gtatgctgca gaagttttgc cgtcgctgcg
     2881 gaatgcttga tgaccgcacc gcgcggggcg agattgccat gcagcactgc aagcccgcct
     2941 tccggtttga tcgggtcttg ccgcggacgg atcgcagtct ggcccggcac gtcttcggcg
     3001 gcgacaacga cctcgcgcaa ggtcttgcct tcgatggtct tcgcatcgag atcgaggaga
     3061 tcgccgagct gcgccatcag cttcggcacg ccgcccgcat ggtggaaatg ctccatgtag
     3121 tgatcgcccg acggtttgag gtcgaccagc accggcacct cgcggccgag cttgtcgaac
     3181 gcgccgaggt cgatgccatg cggcgtgcgg ttggcaatcg cggtgagatg aatgaggcca
     3241 ttggtcgagc cgccgatcgc ctgcaacacc acctgcgcat tgcgaaacga ggacttggtc
     3301 aggacttcgc tcggcttcgg tcccttcgcc ttggccatgg cggctgcgac cttgccgctt
     3361 gcctcggcaa ggcgaaaacg ctcggcatgc ggcgcgggga tcgtcgcgct cattggcagc
     3421 gacagcccga gcgtttcggt gatgcaggcc atggtgctcg ccgttcccat caccatgcag
     3481 gtgccgaccg acggcgcaag ccgcccgttc accgcctcga tctccttgtc gtcgatctcg
     3541 cccgcgcgat acttcgccca aagtctgcgg caatcggtgc aggcgcccag cacctcgccc
     3601 ttgtgatgac cgactaccat cggcccgacc ggaatgacga cggtcggcag atcggcggag
     3661 acggcggcca tgatctgcgc cggcaaggtc ttgtcgcagc cgccgatcac gatcacggca
     3721 tccatcggct gggcgcggat catctcctcc gtatccatcg ccatcagatt gcgcagatac
     3781 atcgaggtcg gatacgcaaa gctctccgca at
//
LOCUS       DATLJT010000303         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_246405,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000303 DATLJT010000000
VERSION     DATLJT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246405"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..932
                     /locus_tag="VM910_06895"
     CDS             231..932
                     /locus_tag="VM910_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI62305.1"
                     /translation="MPTIALVDDDRNILTSVSIALEAEGYRIMTYTDGASALDGFRTS
                     QPDLAILDIKMPRMDGMETLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAVLRRSQPKDPTAAPKENDAKALDRGLLRMDPERHTCTWKNEPVTLT
                     VTEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDDFE
                     MIETLYGVGYRFKEA"
     gene            1013..>2546
                     /locus_tag="VM910_06900"
     CDS             1013..>2546
                     /locus_tag="VM910_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVI62306.1"
                     /translation="MLDRTSPDPGVNAENASVVLDGPAIESPPIKGWQRPLSWLSRAG
                     QFLFTLSFSSLTRRIVSLNIAGLLALVASILYLSQFRAGLIDARAQSLLVQAEIIAGA
                     IAASATVQTNTITIDPERLLDLKAGESYAGPDEYSPLDFPINPERVAPVLRTLISPTK
                     TRARIYDPNGALLLDSRTLENVLRFDLPPPTGQPGIVGRAMIAIRTWLNRGDLPLYKE
                     LGPENGNGYEEVANALKGQKRSMVRVNSRGEVIVSVAVPVLRSRAINGVLMLSTQGDD
                     IDQMVTAERLAILKVGGVASAVMIVLSLLLASTIAGPVRRLADSAERVRRRIKTRVEI
                     PDFTRRRDEIGHLSGALRDMTGALYNRIEAIEMFAADVAHELKNPLTSLRSAVETLPL
                     ARSDSSRARLLEVIEHDVKRLDRLISDISDASRLDAELQRQDMAPVDLRRLLTTLTSV
                     ANETRLGHDVAVEARFEGRSSSDIFLVPGHDSRLGQVISNLLSNAQSFSEPGGKVRIL
                     CRR"
BASE COUNT          470 a          825 c          774 g          477 t
ORIGIN      
        1 ggtcggttgc atcgattatt accccgtcat cctgttgcaa tgagggtctt ttttcggcta
       61 aaccggcatc caaaggggtc aggatgcttc gcggtttcgg agcgttttac ggaactcggt
      121 cacacttcag cccagcttac attgctcgtt gaccgcttcc gaggcccaat tatgcggcgc
      181 atcccggaat gctcgccgtt tcagctaact ccgaatctga aagcaggccc atgccaacaa
      241 tcgctctggt cgacgacgac cgcaacattc tcacctccgt ttcgatcgcg ctggaagcgg
      301 aaggctatcg catcatgacc tacacggatg gtgcctcagc cctcgacggc ttccgcacca
      361 gtcaacccga tcttgccatt ctcgatatca agatgccgcg gatggacggc atggagacgc
      421 tgcggcgcct ccgtcagaaa tccgatctac cggtgatctt cctcacctcc aaggacgagg
      481 agatcgacga attgttcggc ctcaagatgg gcgccgacga tttcatccgc aagccgttct
      541 cgcagcggct tctggtcgaa cgcgtaaagg ccgtgctgcg ccgctcgcag ccgaaggacc
      601 cgaccgccgc gccgaaggag aacgacgcca aggcgctcga tcgcggcctg ctcaggatgg
      661 atccggagcg acatacctgc acctggaaga acgagcccgt gacgttgacg gtgaccgaat
      721 tcctgatctt gcaggcgctc gcgacgcgcc cgggcgtggt gaagagtcgc aacgcgctga
      781 tggacgccgc ctatgacgac caggtctatg tcgacgaccg caccatcgac agccacatca
      841 aacggctgcg caagaaattc aaggtggtcg acgatgactt cgagatgatc gaaacgctct
      901 acggggtggg ttaccgcttc aaggaagcat gatcgggctt tgcgcggttt aacgcactga
      961 cgccgccgtc cgtctggggt aggattcgca atagtcagtc gggtgccagc cattgcttga
     1021 ccgaacttcg cctgatccgg gtgtgaatgc cgagaacgcc tcggtggtgc tggacggccc
     1081 tgccatcgaa agccctccga tcaagggctg gcagcggccg ctgagttggc tgagcagggc
     1141 tggacagttt ctgttcaccc tctccttctc aagcctcacc cgccgcatcg tctcgctgaa
     1201 cattgcgggc ctgctggcgc tggtcgcgag cattctctac ctgtcgcaat tccgcgccgg
     1261 cctgatcgat gcccgtgcgc agagcttgct ggtgcaggcc gaaatcattg ccggcgcgat
     1321 tgccgcttcg gcaacggtac agaccaacac catcaccatc gaccccgagc ggttgctcga
     1381 tctgaaggcc ggcgaaagct atgccggacc ggatgaatat tcgccgctcg atttcccgat
     1441 caatccggag cgcgttgcgc cggtactgcg cacgctgatt tcgccgacca agacccgcgc
     1501 ccggatctac gatccgaacg gcgcactgtt gctcgacagc cggacgctgg aaaacgtgct
     1561 gcgctttgac ctgccgccgc cgacggggca gccggggatc gtcgggcgcg cgatgatcgc
     1621 gatccgcacc tggctcaatc gcggcgacct gcccctctac aaggaactcg ggcccgaaaa
     1681 cggcaacggc tatgaggaag tcgccaacgc cctgaagggc cagaagcgca gcatggtgcg
     1741 cgtgaattcc cgcggtgaag tcattgtctc ggtcgcagtc cccgtgctgc ggtcgcgcgc
     1801 catcaacggc gtcctgatgt tgtcgacgca gggcgacgac atcgaccaga tggtgacggc
     1861 ggaacggctg gcaatcctca aggtcggcgg cgtcgcgtcc gcagtcatga tcgtgctgtc
     1921 gctgttgctc gccagcacga tcgcaggtcc cgtgcgacgg cttgccgact ctgccgagcg
     1981 tgtccgccgc cgcatcaaga cgcgcgtgga gattccggat ttcacgcggc gccgcgacga
     2041 gatcggccat ctctccggcg cgctgcgcga catgacgggc gcgctctaca accgcattga
     2101 ggcgatcgag atgttcgccg ccgacgtcgc tcacgaactg aagaacccgc tgacctcgct
     2161 gcgctccgct gtggaaacgc tgccgctcgc gcgctcggac agcagccgcg cgcggctgct
     2221 tgaagtgatc gagcatgacg tgaagcggct ggatcggttg atatcggata tttccgatgc
     2281 gagccgactc gatgccgagt tgcagcgcca ggacatggcg cccgtcgatc tgcggcgact
     2341 cctgacgacg ttgacctccg tcgccaacga gacccgactc ggtcacgacg tcgcggtcga
     2401 ggcgcgcttc gaaggtcgca gctcgtccga catcttcctg gtgccgggcc atgattcgcg
     2461 cctcggacag gtgatatcca acctgttgtc caacgcccag tccttctcgg agcccggcgg
     2521 caaggtgcgc atcctctgcc gccgcg
//
LOCUS       DATLJT010000304         1999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_246734,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000304 DATLJT010000000
VERSION     DATLJT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246734"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1300)
                     /locus_tag="VM910_06905"
     CDS             complement(20..1300)
                     /locus_tag="VM910_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62307.1"
                     /translation="MTTAKSMTTTSITKSIPRRSVLGGMAAAGALTVARPYVARAQQG
                     DINIGVLLPFTGSQGAYGPDMRKAAELTTKIVNDDAGGVLGGRKFKLFFEDDESSPTA
                     GLAATKKLLEVNKCVAVIGIWGSPIAMAIKPVLVNANTCMMVSGAANAITEGDTKGLV
                     WRFQAKATDWGPAMAQAMLSRGWKTVSILAQQNPFVIPMIEPAKKTFAAGGTKVLDTV
                     IYNPDQPSYRAEVQRIFGANPAPEAVMCLGLLTDFVSIVREAIRMGATSKIMALSIMA
                     DAEGKFIEAVGPDVAEGIEHFQPAPPLGSSSYKKFQKLMGATDENALYLFAGNTHDQI
                     CTLALAMEKAKSTDPLVYTKEIPAVDNPPGEEIDEILVALKKVREGVKINFQGAGSIC
                     DFDAHGDQLNRHYGHFRIEKGKQVLVQIIKGPTG"
     gene            complement(1343..1888)
                     /locus_tag="VM910_06910"
     CDS             complement(1343..1888)
                     /locus_tag="VM910_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI62308.1"
                     /translation="MARVGFVPASDLPAHVARIYEQYSTGYGPFRNQVAVFAHVPSAV
                     EHIMGLLLDLKRQRNLNWRYVELAIVVTSKLNACHYCVAHHTAPLKVEGLSEAAIEML
                     PAVHEELDEVDRLVVDFTVAVTNNARRIPERMFEALRAHFSEAQIVELTLRIALCGFF
                     NRFNDALQIDNELEEAVHAAK"
BASE COUNT          348 a          608 c          628 g          415 t
ORIGIN      
        1 tccgcggagt aaccggaatc tacccagtcg gccccttgat gatctggacc agcacctgct
       61 tgcccttttc gatgcggaaa tggccataat gccgattgag ctggtcgccg tgagcgtcga
      121 agtcgcagat cgatccggcc ccttgaaagt tgatcttgac gccttcgcgc actttcttga
      181 gggcaacgag aatttcgtcg atttcctcgc ctgggggatt gtcaaccgcc ggaatctcct
      241 tcgtgtatac cagcgggtcc gtgctcttgg ctttctccat tgccagcgcc agcgtacaga
      301 tctgatcgtg cgtgttgccg gcgaacaaat agagcgcgtt ctcgtcggtc gcgcccatca
      361 gcttctggaa tttcttgtag ctcgagcttc cgagcggcgg ggccggctga aaatgctcga
      421 taccttccgc gacgtccggt ccgacggcct cgatgaactt tccttcggcg tcggccatga
      481 tggacagcgc catgatcttg ctggttgcgc ccatgcggat cgcctcgcgc acgatggaca
      541 cgaaatcggt gagcaggccg aggcacatca cggcttcggg cgcggggttt gctccgaaga
      601 tgcgttgcac ctcggcccga taggacggtt ggtcggggtt gtagatgaca gtatccagca
      661 ccttggttcc gcccgccgca aaggtctttt tcgccggctc gatcatggga atgacgaagg
      721 gattctgttg cgccaggatc gacacggtct tccatccgcg tgagagcatc gcctgcgcca
      781 tggccggtcc ccagtcggtc gccttggcct ggaagcgcca gaccaggcct ttcgtgtcac
      841 cctcggtgat ggcgttggcc gcgccggaaa ccatcatgca ggtattggca ttgacgagca
      901 cgggcttgat cgccatcgcg atcgggctgc cccagatgcc gatgacggcc acgcatttgt
      961 tgacctcgag cagcttcttg gtggcggcaa ggccggccgt tgggctcgat tcgtcgtcct
     1021 cgaagaacaa cttgaatttg cggccgccga gaacaccgcc ggcgtcgtcg ttgacgatct
     1081 tcgtcgtcag ctcggccgcc ttgcgcatgt ccggcccgta ggcgccctgc gaaccggtga
     1141 acggcagcag gaccccgata ttgatgtcgc cttgctgcgc ccgtgcgacg taaggccgag
     1201 ccaccgtaag ggcgcccgcg gccgccatgc cgccgagtac gctacggcgg ggaatggatt
     1261 tggtgatgga cgtcgtggtc atgctcttgg ccgtggtcat gatcggtttc ctttcctccc
     1321 aacaaagcat attcgggaat tctcacttgg cggcgtggac cgcttcctcg agctcgttgt
     1381 cgatctgcag ggcgtcgttg aagcggttga agaaaccgca cagggcgatg cgcaatgtga
     1441 gttcgacgat ctgcgcctcg ctgaaatgcg cgcgcaatgc ctcgaacatc cgctcgggga
     1501 tgcgccgcgc gttgttggtc accgccaccg tgaaatcgac gacgagccgg tcgacctcgt
     1561 cgagctcctc gtgcaccgcc ggcagcatct cgatcgcggc ctccgacagg ccttcgactt
     1621 tcagcggcgc ggtatgatgg gcgacgcaat agtgacaggc gttgagcttg gacgtcacca
     1681 cgatcgccag ctcgacgtag cgccagttca aattgcgctg tcgcttgagg tcgaggagga
     1741 ggcccatgat gtgctccacc gcggagggca cgtgcgcgaa caccgccacc tggttgcgga
     1801 acggtccgta accggtcgaa tattgctcgt agatgcgggc gacatgagcc ggaagatcgc
     1861 tcgcgggaac aaagcctacg cgcgccatgc ggtcctccct tcaggatgcg ctctgggata
     1921 ccgacggccg ctcgcggacg agcgacgtgg tcttgagccg atattcgggc aggaagcctt
     1981 gcggccgata aatcaagaa
//
LOCUS       DATLJT010000305        11407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2084851,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000305 DATLJT010000000
VERSION     DATLJT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084851"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VM910_06915"
     CDS             complement(<1..702)
                     /locus_tag="VM910_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI62309.1"
                     /translation="MRMETYRYEELARFITALVDGGTLTPGTRAPSLREISRDRNVSM
                     ATALQAYRLLEDRGILQARPQSGFYVARRPSPRKAPTISRPPARATDVSVSDVIVKLL
                     VHASDPDLVPLGCAIPSADLLSAGRLDRLLARTARERGGAYNTYTGPQGDVRLRREIA
                     RRALRWDRALSPADLVVTVGCTEALVLALRAVARPGDTIAIESPTYFGLLHALEALEL
                     KAFELPTDATEGIDLN"
     gene            805..1935
                     /locus_tag="VM910_06920"
     CDS             805..1935
                     /locus_tag="VM910_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVI62310.1"
                     /translation="MPQFVKNDIISLVSAAPRHDLAESVGPDLRLGELLGADGGWDET
                     RLGYATAAGAPVLRAAIAGLHGVDANDVVVTVGGIHALFLLGYILCGRGEEAVISTPV
                     FPPARDILVSVGAKLRVLPAGFDDRYRLDPEKLARLLSPATRLVSLASPQNPSGVALP
                     RAVLSEVLSAMADRCPDAFLLIDETYRCAAFGDDAIAPSGATMSPRVVVTGSLSKCHG
                     VPGVRIGWAITRNAELREQLVIGKFSTVISNSAVDEMLGLRVLRRADEIIGDRRRRLG
                     DGLARTATWVERNAALVEWVRPDAGALCCIRLRPDVFDDAGVMRFYAGLSRRDARVGD
                     GSWFGEEPRVFRLGFGFLAPDELDTALDVLTATLQQARRNAA"
     gene            1966..2823
                     /locus_tag="VM910_06925"
     CDS             1966..2823
                     /locus_tag="VM910_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HVI62311.1"
                     /translation="MPTLAFHQVDVFTALPLKGNPLAVVPDADELSDETMASLANWTN
                     LSETTFLLKPTVPGADYRVRIFTPVGELPFAGHPTLGSCHVWLNRKGTAKQSRIVQEC
                     GVGLVQIKRDGDRLAFAAPPLRRSGDVDRATIEIIASGLKIRPETIKAAQWVDNGPGW
                     VAVMLSTRAEVLALKPDYAALAGHMLGVIAPWEAARDGNEAQFEVRAFIPSRASEDPV
                     TGSLNAGLAQWLTSAGLAPNAYIASQGTALGRAGRVYVERDGENVWIGGNTVNCIEGR
                     LDLGSLGPG"
     gene            2999..3925
                     /locus_tag="VM910_06930"
     CDS             2999..3925
                     /locus_tag="VM910_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI62312.1"
                     /translation="MGAKRPSLNALRALEATVRLSSMTAAAEELSVTHGAISRHIKSL
                     EEMFGIPLLLRGARSVQATPEGGRLATELSRAFDLIASSVEQLQPGPLTLSCSSTIMM
                     SWLIPRIGAFHELHPDIELRFNMNYDRIDFVRDEISVAIRISMIEPPKEVIIKDMIDE
                     WIGPVCSPEYASKHFKTARDLDRCALLATKTRPGGWAHWARISGQSRELRPQRFYDHF
                     YLLIQAAACGLGVAMVPKILVLDDLRSGKLVAPFGFVAGPFKIVLWIAPHLRARSETK
                     ALVDWLTQEFRKPGMQGDEPAPANALQAASRR"
     gene            complement(3952..4812)
                     /locus_tag="VM910_06935"
     CDS             complement(3952..4812)
                     /locus_tag="VM910_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62313.1"
                     /translation="MSKASSHFADLDGVTLHYRRAGQGEAVVLVHGIPQTSHEWRYVM
                     PRLAEKYTVIAPDLRGLGDSTRPSGAYDKKTLGADIAALVIKHLGFDKFHLVGHDWGG
                     PVAFSVAAHNPDAVRTLTILDVVIPGDGGDFSQGGRRWHHAFFRTLDLPEQLCFGREE
                     MVINWLFENYGYRANCISDEDKAEFLRTYKKPGAFRALLEIYRALPRDAEDNRNILAR
                     NGKLKMPVLALGGEKSFGRGAECIDSLRRVAENVRGGLVRDCGHWIAEEQPEFLAKEL
                     LGFFGEFPIH"
     gene            complement(4820..5836)
                     /locus_tag="VM910_06940"
     CDS             complement(4820..5836)
                     /locus_tag="VM910_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62314.1"
                     /translation="MTTLSRRSFTALAGLATIGAAVPLRYAKAAEFSFKLGTNVPETH
                     PLNVYAKKAAEELAQTTDGRFELQIFANNQLGGDSDMFSQLRSGALECFTLSGVNVLS
                     TLIPSAAIWGVGFAWKDYPTLWNALDGKFGAHLRSQIDKAGLVVMDKIWDNGFRQVTT
                     SVRPIVTPADFQGLKIRVPVSALWTSLFKSLGASPTGINFAEVYSALQTKIVDGQENP
                     PAIISAAKLYEVQKYCSLTNHMWDGWWFLVNRRAWQSVPRDLQEKVAAVINKNAVAER
                     EEIARQNTSLKSDLAAKGLIFNDVDPAPFRETLSKSGFYSEWKSKFGEEAWALLEEAT
                     GKLG"
     gene            complement(5865..7232)
                     /locus_tag="VM910_06945"
     CDS             complement(5865..7232)
                     /locus_tag="VM910_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HVI62315.1"
                     /translation="MPRPLPSGPLIAFYGDDFTGASASMEATAFAGLDTVLFLSPPPA
                     DRLRTFASARVIGIAGVARAQSPQWMDDNLPPIFDLLGATGAPIVHYKVCSTFDSAPH
                     IGSIGRAIEIGSRRFDGWTPMIVADPGMGRYQACGNLFAAVRGDVYRLDRHPVMSRHP
                     VTPMDEADLGRHLARQTKQKIGLVDFVAMKRGEADTRLAHELAAGAKIVSIDVLDQET
                     LIEAGRLVWERGDCAGPLYGVGSQGFEAALVSYWQHSGLVPPPKSAFHAQPVDRIACV
                     SGSVSPVTAAQIAHARDHGFSTIALDAARAVDPVEWKREIGRAADAALAQLDSGRDTL
                     VYSAAGPDDPAVASLKTALASSGISPETVNERMGAGLGTILDRILLESGLSRAVISGG
                     DTSGRAASMLGIDALTAVAPLAPGSPLCRAHAARPDRDGLEIALKGGQVGAPDFFVAA
                     KRGGS"
     gene            complement(7235..8497)
                     /locus_tag="VM910_06950"
     CDS             complement(7235..8497)
                     /locus_tag="VM910_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit
                     family protein"
                     /protein_id="HVI62316.1"
                     /translation="MPGSRIEADYLLETPDDPSRVAELMAGEQSSGTFVAVPGETEEL
                     KSRVAARVERLQVVDETVTAPSLPSARINIVPGARFRRAHVTLSWPIDTLGPSLPNLL
                     ATVAGNLFELKPVTGLRLLDIRLPPEFGAAYPGPRFGVAGTRHLAGVDGRPLIGTIIK
                     PSVGLGPDETAAVVNDLCRAGIDFIKDDELQSDGPACPFDRRVAAVMRVINDHAERSG
                     KKVMYAFNLTGDLDQMRRRHDTLLKHRATCLMASLNSVGLAGMIELARFSQLPIHAHR
                     NGWGYLSRHPQLGWSYIAWQKIWRLAGADHMHVNGVGNKFAESDESCLASARACLTPM
                     FEDKLCTVMPVFSSAQSPRQAPATYQGLGSADLIVTAGGGIMAHPLGTAAGVSAMQEA
                     WEATLAGVPLAEFARTRPNLQRALEMAA"
     gene            complement(8528..9322)
                     /locus_tag="VM910_06955"
     CDS             complement(8528..9322)
                     /locus_tag="VM910_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62317.1"
                     /translation="MPGRLQDKIAIVFGAGSVGPGWGNGKATATLFAREGAHVVCVDF
                     NPEAAKETVEIITGEGGSASAAICDVTRSEQVKQVVDDIAARHGRIDVLHNNVGYATI
                     GGPVELSEDEWHKTIDLNITGCFITCKHVLPDMLGRRSGAIVNVSSIAAIRWTGYPYA
                     AYYAAKAAVNNFTMGLALQYAKDGIRANAVMPGLMNTPLIHQQISGQYKDAEDMVRAR
                     DAACPMGRMGTAWDVARAALFLASDEASYITGVSLPVDGGLTCRAA"
     gene            complement(9398..10405)
                     /locus_tag="VM910_06960"
     CDS             complement(9398..10405)
                     /locus_tag="VM910_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62318.1"
                     /translation="MRINRRTFVLGTAAVGITAPWVARAAGPEYRLKFGNIVSGDHPL
                     NTAMAKAGERIKTETDGQVTIELFPKNQLGSDADMLSQLRSGALELFAQTGVLMSTLV
                     PVASINGIGFAYPTYDKVWESLDGPLGSHIRKAFSNANLVCLEKSWNHGFRQVTSSTK
                     PILTPDDFNGFKIRVPPSPLWTAMFKAFGAAPVSIPWAETYSAMQTHIADGLEQPLIG
                     LLVDKMYEVQKHCSLTNHMWDGFWILSNKRVWEGIPANLREIVERNINEEAIRQRAEV
                     ENMNATLQAELQNRGLEFHKVDNAAFRDKLVAAGFYAEWKKRYGEEAWALLESTTGKL
                     G"
     gene            complement(10480..>11407)
                     /locus_tag="VM910_06965"
     CDS             complement(10480..>11407)
                     /locus_tag="VM910_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HVI62319.1"
                     /translation="DMAAVAPVLFPEMRLRGAKDGDLVALLSATGAQTETIPPSIVLI
                     TIGSVTGVSISALFTGGMLPGLVLGVTLCFLVWWRYRSEDLSAVRRPTRQVILRSLAV
                     AFPALLLPFVIRAAVVQGVATATEVSTIGIFYAVVVGLFIYRQFPLNRIYPMLVTTAG
                     LSGAILLIIGAATGMAWALTQSGFSATLAAALKSLPGGAPVFLVVSALAFVVLGSVLE
                     GIPAIVLFGPLMFPIARQVGVHEVHYAMVVILAMGIGLFSPPFGVGYYAACAISRINP
                     DEGIRPIVGYILALIVGLAFVIAIPWISTGFL"
BASE COUNT         2202 a         3559 c         3581 g         2065 t
ORIGIN      
        1 attgaggtcg atgccttccg tcgcatcggt cggaagctcg aatgccttga gctcgagtgc
       61 ctcgagcgca tgcagcaggc cgaaataggt cggcgattcg atcgcaatcg tgtcgcctgg
      121 tcgcgcgacc gcacgcaatg cgagcacaag cgcctcggta catccgactg tgacgacgag
      181 atcggccggc gaaagcgccc ggtcccagcg cagcgcacgc cgcgcaatct cccgccgcag
      241 ccgaacgtct ccctgcggcc cggtgtaggt gttataggcg ccgccccttt cccgcgcggt
      301 gcgcgcaagc agccgatcaa gccggccggc gctgagcaga tcggcgcttg ggatcgcgca
      361 gccgagcggt acgagatcgg ggtcggaggc atgtacaagc aacttgacga tgacgtcgga
      421 gacgctgaca tcggtggcgc gcgccggcgg ccgtgagatt gtcggcgctt tccgcggcga
      481 gggccggcgg gcaacgtaaa agccggactg cgggcgagcc tgcagaatgc cgcgatcctc
      541 gagcagacgg taagcctgca gagcggtcgc catgctgaca tttcggtcgc ggctgatctc
      601 gcgcagcgat ggcgcgcgcg tgcccggagt caaagtgccg ccgtcgacca gcgcggtgat
      661 gaagcgcgcg agctcttcgt atcgatacgt ttccatgcgc atggggctac cataactgta
      721 ctggtcgtga ttgtcatcct ctgtcactgt actggtcagg gccggcctgc tacgccggaa
      781 ttggatcggc gacggaggag agcaatgccg cagttcgtca agaacgacat catttcgctg
      841 gtcagcgccg cgccgagaca tgatcttgcc gaaagcgtcg gtccggacct tcgactcggc
      901 gaactcctcg gcgcggacgg tggctgggac gaaacacggc tcggctatgc gacggcggcg
      961 ggcgccccgg tgttgcgcgc ggccatcgcc ggtctccacg gcgtcgacgc caacgatgtc
     1021 gtggtcaccg taggcggcat ccatgcgctg ttccttctgg gttacatcct gtgcggccgc
     1081 ggcgaggaag cggtgatcag cacgccggtg tttccccccg cgcgcgacat cctcgtaagc
     1141 gttggcgcga agctccgcgt actgccggcc ggctttgacg accgctaccg gctcgatcct
     1201 gaaaaactcg ctcgcctgct gtcgcccgct acgcggctcg tgtccctcgc ctcgccgcaa
     1261 aatccttcag gcgtcgcgct gccgcgcgcg gtgctgagcg aagtcctttc ggcgatggcc
     1321 gaccgctgtc ccgatgcgtt cctgctcatc gacgaaacct accggtgcgc cgcctttggc
     1381 gacgacgcga ttgcgccgag cggagcgacc atgagcccgc gggttgttgt gaccggctcg
     1441 ctgtcgaagt gccacggcgt gcccggcgtt cgcatcggct gggcgatcac ccgcaacgcg
     1501 gagctgcgcg aacaactggt gatcggcaag ttcagcaccg tgatctccaa ttcggccgtc
     1561 gacgagatgc ttggactgcg cgtgctgcgg cgcgcggacg agatcatcgg agatcggcgg
     1621 cgcaggcttg gcgatggact ggcgcgcacc gcaacgtggg tcgaacgcaa tgccgcactc
     1681 gtggaatggg tccgaccgga cgccggggcc ttgtgctgta ttcggctgcg gccggacgtg
     1741 ttcgacgacg ccggtgtcat gcgcttctat gctggcctgt cgcgccgcga cgcccgggtc
     1801 ggagacggta gctggtttgg cgaggagccg cgcgtctttc gtctcggctt cggctttctt
     1861 gcgccggacg agttggatac ggcccttgac gtactgaccg cgaccttgca gcaggcccgc
     1921 cggaatgcgg cctgaggaga acaaacaaga gagaggccaa attcgatgcc tacacttgcc
     1981 ttccaccagg tcgacgtttt caccgccttg ccgctgaagg gaaatccgct ggctgtcgtt
     2041 cccgatgccg acgaactttc cgacgagacc atggcctcgc tcgccaactg gacaaacctg
     2101 agcgagacca cgtttctgct gaagccgacg gtgcccggcg ccgactatcg cgtgcgcatt
     2161 ttcacgcccg tgggcgagtt gccctttgcc ggccacccga cgctcgggag ctgccacgtc
     2221 tggctgaacc gcaagggcac ggcgaagcag tcccgtattg tgcaggaatg tggcgtgggt
     2281 ctcgtgcaga tcaagcggga cggcgaccgc cttgcgttcg cggctccgcc gcttcgccgc
     2341 agcggcgacg tcgaccgcgc aacgatcgag atcattgcaa gcggcctgaa aatacgaccc
     2401 gaaaccatca aggccgcgca atgggtcgac aacggtccgg gctgggtcgc ggtgatgctg
     2461 tccacacgcg ccgaggtttt ggctctgaaa cccgattatg ccgcgctggc tggccacatg
     2521 ctcggcgtca tcgcgccttg ggaggctgcg cgagacggca acgaagcgca gtttgaagtg
     2581 cgcgccttca tacccagtcg cgcctcggaa gacccggtga cgggcagcct caacgcaggg
     2641 ctcgctcaat ggctgacatc ggcggggctc gccccgaacg cctatattgc gagtcagggg
     2701 acggcgctcg gtcgcgcggg tcgcgtttat gtcgagcgcg acggggaaaa cgtgtggatt
     2761 gggggcaaca cggtgaattg cattgaaggc aggctcgacc ttgggagcct tggacctggt
     2821 taagcccgaa cttcaggaag gcgtcacttc cttgtcgatt gacccgccct atcacagagc
     2881 tccactgaga gcgccttaac aaccgcgtca aagcgcacga ccgcttgcaa agccctgacg
     2941 caagcggtag ctatggaact gtgaaaaaag ctcacaacag cggcaaggga gggccgcctt
     3001 gggagcaaag cgcccatctc tgaatgcgct gcgcgcactg gaggcgacgg tccgcctttc
     3061 cagcatgacc gctgccgccg aagaattgtc ggtcacgcac ggggccatca gccgacacat
     3121 caaaagcctc gaagaaatgt ttggcattcc gctgcttttg cgcggtgcac gctcggtcca
     3181 ggcgacgccg gaaggaggac gtcttgccac cgagctgtcg agagcctttg acctgattgc
     3241 atccagcgtc gaacagcttc agcccggtcc actgacgctg tcctgctcgt cgacgatcat
     3301 gatgtcctgg cttattcccc gcatcggcgc gtttcacgaa ctgcatccgg acatcgaact
     3361 gcgcttcaac atgaactatg accggatcga tttcgtacgc gacgaaatca gcgtcgccat
     3421 aagaatcagc atgatcgagc ctccgaaaga ggtgatcatc aaggacatga tcgacgagtg
     3481 gattggcccg gtctgctcgc cggaatacgc ctcaaaacac ttcaagacgg cgcgcgatct
     3541 cgatcgatgc gcgctcctgg caacaaagac gcgtccgggc ggctgggcac attgggccag
     3601 gatatcgggt caatcccgcg aattgcggcc gcaaagattt tacgatcatt tctatctgtt
     3661 gatacaggcg gccgcgtgcg gtctcggcgt cgccatggtg cccaagatcc ttgtcctgga
     3721 cgatcttcgc tcaggcaagc tggtagcgcc gtttggtttc gtggcgggtc cgttcaagat
     3781 cgtgctctgg atcgcgccgc atctgcgcgc gcgatccgaa accaaagcgc tcgtcgattg
     3841 gctcacgcag gagttccgta aaccgggcat gcagggtgat gagcccgcgc cagcgaacgc
     3901 cctgcaagcg gcctcccggc gatagcgatt gcgaggctaa ctccacccac ctcaatggat
     3961 gggaaactcg ccgaagaagc cgagaagctc tttggcaaga aattccggct gctcctcagc
     4021 gatccagtgc ccgcaatccc tgaccagccc gccacgcacg ttctctgcca cccgccgaag
     4081 cgagtcaatg cattcggcgc cgcggccaaa gctcttctcg ccaccgagcg caagtacagg
     4141 catcttcagc tttccattgc gcgcgaggat gttcctgttg tcttcggcat cccgtgggag
     4201 agctcgatag atttcgagca gtgcccgaaa tgcaccgggc ttcttgtacg tccgcaaaaa
     4261 ctcggccttg tcctcatcgg aaatgcagtt tgcacgatag ccgtagtttt cgaatagcca
     4321 gttgatgacc atctcctcgc gcccgaagca caactgctcc ggcaaatcga gcgtgcggaa
     4381 gaaggcatgg tgccaccgcc gcccgccctg ggaaaaatcg ccgccgtcgc cgggaatgac
     4441 gacgtcgagg atggtaagcg ttctgaccgc gtccggattg tgcgctgcga ccgagaaggc
     4501 aaccgggccg ccccaatcgt ggccgacgag gtgaaatttg tcgaacccga gatgcttgat
     4561 gacaagggcc gcgatgtcgg cgccgagcgt cttcttgtcg taggcgcccg aaggccgcgt
     4621 cgaatctcca agcccgcgca aatcgggagc gatgacagtg tatttttctg caagtcgagg
     4681 catgacgtac cgccattcat gcgacgtctg cggaatgccg tgcaccagta cgacggcttc
     4741 cccctgcccc gctcgacgat aatgcagggt tacgccgtcg aggtccgcga aatggctgct
     4801 cgcttttgac atgagagtgt tagcccaatt taccagtggc ttcttcgaga agcgcccagg
     4861 cttcttcgcc gaacttcgac ttccattcgg aatagaagcc ggacttgctt agcgtctctc
     4921 taaagggtgc aggatcaacg tcgttgaaga tgaggccctt ggcggcaaga tcgctcttga
     4981 gcgacgtgtt ctgccgggca atctcttcgc gctcggccac ggcgttcttg ttgatgaccg
     5041 ccgccacctt ctcctgtaga tctcgaggaa cgctctgcca cgcgcgccgg ttcacgagga
     5101 accaccagcc atcccacatg tggttggtca gtgagcaata tttttgcact tcgtagagct
     5161 ttgcggcaga gatgatggct ggcggattct cctggccatc gacgatcttg gtctggagag
     5221 ccgaatagac ctccgcgaag ttgatcccgg tcggcgaagc cccgagcgac ttgaacagcg
     5281 aggtccagag tgcgctaacg ggaacgcgta tcttgagccc ctgaaaatcg gcgggcgtaa
     5341 cgatcggccg aacgctggta gtgacctgcc tgaaaccgtt gtcccagatc ttgtccatga
     5401 ccaccaggcc ggctttgtcg atttgtgacc gcagatgtgc accgaacttg ccatcaagcg
     5461 cattccagag cgtcggatag tctttccagg caaagccgac accccagatc gctgccgatg
     5521 gaatcagcgt cgaaagaacg ttgacgccgg acagggtaaa acactcgaga gccccggagc
     5581 gaagctgcga gaacatgtcg gaatcgccgc cgagctgatt gttggcaaat atctgcaatt
     5641 cgaaccggcc atctgtcgtc tgtgcgagtt cctctgcagc cttcttggcg tagacgttga
     5701 gcggatgcgt ttccggcacg ttggtgccca acttgaacga gaattcggcc gccttcgcgt
     5761 atcgcagcgg aacggccgcg ccgatcgtgg cgagtcccgc aagcgcagtg aaactgcgac
     5821 gagagagtgt ggtcattttt tcctccggac aaactgatga agcatcagga accaccacgc
     5881 ttggcggcga caaaaaagtc gggggcaccg acctgtcccc ccttaagggc gatctcgagc
     5941 ccatcgcggt cgggccgcgc ggcatgggcg cgacaaaggg gcgaacctgg cgcgagcggc
     6001 gctacggctg tgagcgcgtc gatgccgagc atcgatgctg cgcggccgga tgtgtcgccg
     6061 ccggaaatca cggcacgcga caaaccggat tccagcagaa tccgatcgag gatcgttcca
     6121 agaccagcgc ccatacgctc gttcacggtt tccggcgaaa tgccgctcga tgcgagggcg
     6181 gtctttagcg aagctacggc cggatcatcc ggtcctgcgg cgctgtacac cagcgtgtcc
     6241 ctacccgaat ccagttgggc aagcgccgcg tcggcggcgc gaccgatttc ccttttccat
     6301 tccaccggat ccaccgcacg cgcagcgtcg agcgcaattg tcgaaaagcc gtggtcccgg
     6361 gcatgggcga tctgtgcggc ggtgacggga gagaccgagc ccgacacgca ggcgatgcga
     6421 tcgaccggct gcgcatggaa cgctgacttg ggcggcggga ccagcccgga gtgctgccag
     6481 tacgagacga gcgcggcctc gaacccctgc gagccgacgc catagagagg cccggcacaa
     6541 tcgccgcgct cccacacgag acggccggct tcaatcaagg tttcctggtc gagaacgtcg
     6601 atcgagacga tttttgcgcc ggcggcaagc tcatgcgcga gccgagtatc ggcctctccg
     6661 cgcttcatcg ccacgaaatc gaccagacct atcttctgct tcgtctggcg cgccagatga
     6721 cggccaagat cggcctcatc catcggcgtt accggatgac gcgacatcac cggatgacgg
     6781 tcgaggcgat agacatcgcc gcgcaccgcc gcgaacaggt ttccgcatgc ctggtagcgg
     6841 cccatgcccg gatcggcgac gatcattggc gtccagccat cgaaacgccg cgatccgatc
     6901 tcgatggcgc ggccaatcga gccaatgtgc ggagccgagt caaatgtcga gcacaccttg
     6961 taatgaacga tcggcgcgcc ggttgcgcca agaagatcga agatcggcgg caagttgtcg
     7021 tccatccatt gcgggctctg agcccgcgcc acgccggcta ttccgataac gcgagcgctt
     7081 gcaaaggtgc gcaagcggtc cgcgggcggc ggcgacaaga acaggaccgt atcgaggccg
     7141 gcgaatgccg tcgcctccat cgaggccgat gctcccgtga aatcatcacc gtagaacgcg
     7201 atgagaggtc cagacggcaa tggtcggggc atggtcatgc cgccatctcg agggcacgct
     7261 gcaggttggg gcgggtgcgc gcaaattcgg cgagcggaac acccgcgagc gttgcttccc
     7321 aagcctcctg catggcgctc acgccggccg ccgttccgag ggggtgcgcc atgatgccgc
     7381 cgccggcggt gacgatgaga tcggccgagc ccagcccctg atatgtcgcc ggcgcctgtc
     7441 gcggagattg tgccgaggag aacacaggca tgacagtgca cagcttgtct tcgaacatcg
     7501 gcgtgaggca tgcgcgcgcc gaggcgaggc agctttcgtc gctctccgcg aacttattgc
     7561 cgacgccatt gacgtgcatg tgatccgcgc cggcgaggcg ccatatcttt tgccaggcga
     7621 tgtaggacca gccgagctgc ggatgacgtg atagatagcc ccatccattg cggtgggcat
     7681 ggatcggcaa ttggctgaaa cgcgcgagct cgatcattcc agcaagaccg acggaattga
     7741 gactcgccat caggcaggtg gccctgtgtt tcaagagcgt atcgtgccgc cggcgcattt
     7801 gatcgagatc gccggtcaga ttgaaggcgt acatgacctt cttgcccgac cgctcggcat
     7861 gatcgttgat cacgcgcatg acggctgcga cgcggcggtc gaagggacag gcggggccgt
     7921 ccgattgcag ttcgtcgtcc ttgatgaagt caatgccggc gcggcaaagg tcgttgacga
     7981 ccgccgccgt ctcgtccgga ccgaggccga cgctcggctt gatgatcgtg ccgatcagcg
     8041 gccgtccatc gacccccgca agatgccgtg ttccagcgac gccaaatcgc ggcccgggat
     8101 aggccgcacc gaactccggc ggcaaacgga tgtcgagcaa gcgcagaccg gtcaccggct
     8161 tcagttcaaa caggttgccc gcaaccgttg ccagcaggtt cggaagcgaa gggccgagtg
     8221 tatcgatcgg ccatgacagg gtgacgtgcg cgcgccggaa acgcgcgccc ggaacgatgt
     8281 ttattcgcgc cgacggcagt gaaggagcag tcaccgtctc atcaacgacc tgtagccttt
     8341 ccacgcgcgc cgccacgcgc gatttcaatt cttcggtttc gccgggaaca gctacgaacg
     8401 taccgctcga ttgctcgcca gccatcaact cggcgacgcg actcggatca tcgggcgtct
     8461 cgagcaaata gtcggcctcg atgcgactcc ccggcatttc agatcgctcc atcaaaggct
     8521 gcggccgtca cgccgcacgg caggtcaggc caccatcgac cggcaggctg acaccggtaa
     8581 tgtaggacgc ttcgtcggaa gcgaggaaca gcgcggcacg cgcaacgtcc cacgcggtac
     8641 ccatgcgtcc catcgggcac gcagcatctc gggcgcgcac catgtcctcg gcatccttgt
     8701 attgcccgga gatctgctga tggatgagcg gcgtgttcat caggccgggc atcacggcat
     8761 ttgcgcggat gccatccttg gcatactgca gcgcgagccc catggtgaag ttgttcaccg
     8821 ccgccttcgc cgcgtagtag gcggcatatg gataacccgt ccagcggatc gccgcgatcg
     8881 acgatacatt gacgatagcg cccgaacggc gacccagcat atccggcagc acatgcttgc
     8941 aggtgatgaa gcaccccgtg atgttcagat cgatcgtctt gtgccactcg tcttcactca
     9001 gctcgacagg accacctatg gtggcatagc caacgttgtt gtgcagcacg tcgatccgac
     9061 cgtgcctcgc ggcgatatcg tctacgacct gcttgacctg ttcggatcgc gtgacatcgc
     9121 agatcgcagc gctcgccgac ccgccttcgc ccgtgatgat ctcgacagtt tcctttgccg
     9181 cttccggatt gaaatccaca caaacgacgt gcgcgccttc gcgggcaaaa agcgtcgccg
     9241 tcgcctttcc gttaccccaa cccgggccga cggagccggc cccgaatacg attgcgattt
     9301 tgtcttgcag gcgaccgggc atgatgagtc tcaaaatcct gagttgactt gatgaggtcg
     9361 ggaacgagcc aagccaaaat tccttgcgat ctggcattca cccgagcttg ccggtcgtcg
     9421 actcgagcag tgcccaggct tcctcgccgt agcgcttctt ccactcggca tagaagccgg
     9481 cggcgacgag cttgtcgcgg aatgccgcgt tgtccacctt gtggaattcg aggccccgat
     9541 tctgcagttc agcctgcagc gtcgcattca tgttttccac ctcggcgcgc tgacggatcg
     9601 cctcttcgtt gatgttgcgc tcgacgatct cgcggaggtt tgcgggaatg ccttcccaca
     9661 cgcgcttgtt ggacaggatc cagaaaccgt cccacatgtg gttggtgagc gagcagtgct
     9721 tctgcacctc gtacatcttg tcgacgagaa ggccgatcag aggttgctcg agaccgtcgg
     9781 cgatgtgggt ctgcatggcg gaataggttt ccgcccaggg gatcgacacc ggcgcggcgc
     9841 cgaacgcctt gaacatcgcc gtccatagcg ggctcggcgg gacgcggatc ttgaagccgt
     9901 tgaaatcgtc gggcgtgaga ataggctttg tggacgacgt cacctgccgg aagccatggt
     9961 tccaggattt ctcgaggcac acgagattgg cattggagaa tgccttgcgg atgtggctgc
    10021 cgagcgggcc gtccagcgac tcccagacct tgtcataggt cggataggca aagccgatac
    10081 cgttgatcga ggcgaccgga acgagggtcg acatcagcac gccggtctgc gcgaacagtt
    10141 cgagcgcccc cgaccgcaac tggctcaaca tgtcagcatc ggagccaagc tggttcttcg
    10201 ggaacaattc gatcgttacc tgcccgtcgg tctccgtctt gattcgctcg ccggccttgg
    10261 ccatcgccgt gttgagcgga tgatcgcccg aaacgatgtt gccgaacttg agccggtatt
    10321 cgggcccggc agcgcgtgca acccaaggtg ccgtgatgcc gaccgctgcc gtacccagga
    10381 cgaatgtgcg acggttgatg cgcatgatgg tttcctcaag ttgggcccga agcgcaagcg
    10441 cttcggggtt ggatgttgct attgagcgcg tgggttgcgt tacaggaagc ccgtggaaat
    10501 ccaggggatc gctatgacga acgccagacc gacgatgaga gcgagaatgt agccgacgat
    10561 cggtcttatc ccctcgtctg gattgatccg gctgatcgcg caggccgcgt agtaaccaac
    10621 tccgaacggc ggcgagaaca ggccgatgcc catcgccagg atgacgacca tcgcgtagtg
    10681 cacttcgtgc acaccgacct gccgcgcgat cgggaacatc agcgggccga acagcacgat
    10741 ggcgggtatg ccttcgagaa ccgagccgag cacgacaaac gcgagtgcgg atacgacgag
    10801 aaagaccggc gcaccgcctg gcagggactt gagagcagcg gccaatgtcg ccgagaatcc
    10861 ggattgggtg agcgcccagg ccatgccggt cgcagcgccg atgatgagaa gtatcgcccc
    10921 ggacagaccg gccgtcgtca ccagcatcgg atagatacgg ttcaacggaa actggcgata
    10981 gatgaaaagg ccgaccacaa cagcgtagaa gatgccgatg gtcgacactt ccgtcgccgt
    11041 cgcgacaccc tggactaccg cggcgcgaat gacaaacggc agcagcagcg ccggaaacgc
    11101 aacagccagc gatcggagaa tgacctggcg ggtcggccgc ctcaccgcgc tcaggtcctc
    11161 gctccgatag cgccaccaca cgaggaagca gagcgtgaca ccgagcacga ggccgggcag
    11221 cattcctccc gtgaagaggg cggagatcga tacacccgtc accgaaccga tggtgatcag
    11281 aacgatggaa gggggaatgg tctcggtctg cgccccggtc gccgacagca gtgcgacgag
    11341 atcgccatcc tttgcgccgc gcagacgcat ctccggaaag agcaccggcg cgaccgcagc
    11401 catgtcg
//
LOCUS       DATLJT010000306         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2774471,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000306 DATLJT010000000
VERSION     DATLJT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774471"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            587..1228
                     /locus_tag="VM910_06970"
     CDS             587..1228
                     /locus_tag="VM910_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HVI62320.1"
                     /translation="MAKVLIIGASRGIGLEAVKAVLKAGHSVRALARSVRRIRVEHPQ
                     LEKMAGDALEMATVKRALTGIDIVFQSLGVSVGLEIILKPTRFFSKATRVLVTAMEEA
                     QVKRLICVTGFGAGDSRGHGGLLYNVAFHLLLGRVYDDKDVQERIVRRSKLDWVIVRP
                     VILTDGPNTKAYRALVDPSDWTCGFISRADVADFCVKQIDDDAFLHKTPVLVS"
     gene            complement(1422..1700)
                     /locus_tag="VM910_06975"
     CDS             complement(1422..1700)
                     /locus_tag="VM910_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HVI62321.1"
                     /translation="MKSPIVKRSIVIAGHKTSVSLEDAFWEGLKEIAGERRMSSSDLV
                     AAIDSDRQHGNLSSAIRLFVLDFHRAQRSEERRSGAHEMIGDTAPGYP"
     gene            complement(1952..3046)
                     /gene="dinB"
                     /locus_tag="VM910_06980"
     CDS             complement(1952..3046)
                     /gene="dinB"
                     /locus_tag="VM910_06980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVI62322.1"
                     /translation="MSEADSRKIIHVDMDAFYASVEQRDDPSLRGRPVAVGYAAKRGV
                     VASASYEARTFGVRSAMPSSTALRKCADLVFVPPRFDVYRAVSHQIRAIFADYTALIE
                     PLSLDEAYLDVTENLRGISTASATATEIRARIFDETGLTASAGISYNKFLAKLASDHC
                     KPNGQFVVTPAMAAAFVEALPIGKFHGVGPVTADKMKRLGIFTGADLKRQSLEFLQQH
                     FGKSGTWYHAIANGEDNRPVVPDRPRKSSGSERTFADDLTDPAAIEAGVLAMADEVWA
                     WCEKTRVSGRTVTVKIKYADFRQVTRSRTLPAMITSQETLRNVSIGLVRTVFPPAKGI
                     RLLGVTLSSFKNTDRSASKQLDLGLVGRSG"
BASE COUNT          646 a         1002 c         1028 g          666 t
ORIGIN      
        1 cgcccatgcc ggtcgggacg gcgtcgcctg aaataaggga attcggaaat tgagaatcgg
       61 aagctcggcc aatgcgccgg tgagccagct cgacaaggca ggcctcaggc gccgccttta
      121 gctgggctcg tctcaggaaa attaccgctt cggcggtggc cacgcgcccc tatttcgcag
      181 gcgcggtctc tttggccggc gttccttcga cgttgctcgg ttcgccttgc ttactagctt
      241 cgttcaagag ctggtgaatc acgcccgcgc gtcgctcttg ctcgatccga gtctgctgcg
      301 ctttcttctg ttcagcgaag aacttgtaat ctatttccca gcacattgtg tcctccatcg
      361 tgacagaacc aacggcatga caacaccgca atacagctta cagggcgaac agatttcgat
      421 caacgtgtgg gcggtttttt tcgagagaca gcctggcacg taagatcgcg ccgcgtgcga
      481 tcgccacgca ttggaatgcg tttcaagccg cccgcccatc ttgcggtgcg gctggctggc
      541 tagcacatga cgtgagccag gcgggtccga ccaagagcat gacgccgtgg cgaaagtcct
      601 gatcatcggg gcgagcaggg gaattggcct tgaggccgtg aaggccgtac tcaaggcagg
      661 gcattccgtt cgtgcgctgg cgcgctcagt ccgaagaatt cgcgtcgagc acccccagct
      721 ggaaaaaatg gccggcgacg ccctcgagat ggccacggtg aagcgcgcgc tgaccggaat
      781 agatatcgtc ttccagtcgc tcggcgtttc ggtcgggctc gaaatcatcc tcaagccgac
      841 gcgattcttc tccaaagcga cgcgagtgct cgtcaccgca atggaggagg ctcaagtcaa
      901 gcggttgatc tgcgtgacgg gcttcggggc gggcgacagc cgcggccacg gcggacttct
      961 ttataacgtg gcgtttcatc tgctgctggg gcgcgtctat gacgacaagg atgtgcagga
     1021 acgcatcgtc cgcaggagca agctcgactg ggtgatcgtg cgtcctgtga tcctgaccga
     1081 tgggccgaac actaaggcct atcgcgcgtt ggtcgatccg agcgattgga catgcgggtt
     1141 catctcgcgc gccgacgtgg cggatttctg cgtcaagcag atcgacgacg acgcctttct
     1201 gcataagacg ccagtgctcg tgagctgacg cgaacgccgt agccgccgcg ggctccgcgg
     1261 cctcatccga attagcgcgc agccgcatcg agaaggcacg caccgaccaa actaaaattc
     1321 cacctcgacc actcagcggg ggctgatcaa tgtttgcgaa tcgacttgag ccgcgtcggg
     1381 gtgctgacga gaggtgatca ccgcactaaa ccagcagcgg gtcaaggata gccgggcgcc
     1441 gtgtcgccta tcatctcgtg cgctccgctg cgcctctctt cggaacgttg ggcgcgatga
     1501 aagtcgagca cgaacagccg aatggccgat gacagattac cgtgttgtct gtctgaatcg
     1561 attgcagcga ccaagtccga cgaggacatg cgacgctcgc cggcgatctc cttcaggccc
     1621 tcccagaatg catcctcgag actgacgctc gtcttgtgac cagcaattac gattgagcgc
     1681 ttgacgattg gagatttcag ggtggttccc ctgtttgcaa gaaatttgca acatccaaga
     1741 tgaccatcta agaacaattt cagagcgttg caaaaatcac acaccgacga cttctatgtg
     1801 caatcatcca cagctccctc gcagacgaaa ctgccgttac agccgagtat cggcgctcct
     1861 gggccggcga gggttgggtg gctgcgcccg ctggtggtcg ccgccctgga agatcgcctg
     1921 ctcagggcgg gcatcgctgg gcgcctcatg ttcatccgct acgtcctacc agacccaggt
     1981 cgagttgctt cgacgcgctc ctgtccgtgt tcttaaaact tgacaacgtc acgccgagca
     2041 accggatgcc tttggctggc ggaaacaccg tccgcacgag gccgatgctc acgttgcgca
     2101 aagtttcctg agacgtgatc atcgcaggca gggttcggct gcgcgtcacc tgccggaagt
     2161 cggcatactt gatctttacg gtcactgtgc ggccggagac ccgcgtcttc tcgcaccagg
     2221 cccagacctc gtccgccatc gccagcacgc cggcctcgat ggcggccgga tcggtcaggt
     2281 cgtcggcaaa ggtcctttcc gatccggagg atttgcgtgg gcggtccggc acgaccggac
     2341 gattgtcctc gccattggcg atggcgtggt accaggtgcc ggacttgccg aaatgctgct
     2401 gcaggaattc taacgactgc cgcttcagat cggcaccggt gaagatgccg agccgcttca
     2461 tcttgtcggc ggtgaccggt ccaacgccat ggaacttacc gatcggcaag gcctcaacaa
     2521 acgccgcggc cattgccggc gtcacgacga actggccgtt gggcttgcaa tggtcggagg
     2581 ctagcttggc gagaaatttg ttgtacgaaa tgccggccga cgcggtcagg ccggtctcgt
     2641 cgaagatgcg ggcgcggatt tctgttgccg tcgcggacgc cgtagaaatg cctcggagat
     2701 tttcggtgac gtcgagatag gcctcgtcga gcgagagcgg ctcgatgagg gccgtgtagt
     2761 cggcgaagat cgcgcgaatc tgatgcgaca ccgcccgata gacatcgaac cttggcggca
     2821 cgaacaccag gtcagcgcat ttgcgaagcg ccgtgctgga cggcatggcc gagcgcacgc
     2881 cgaatgtccg cgcctcatag ctcgcagagg cgacgacgcc ccgcttcgcg gcatagccga
     2941 ctgcgaccgg acggcctcgc aaggacggat catcacgctg ctcgaccgac gcgtagaagg
     3001 cgtccatgtc gacgtggatg atcttgcgtg agtcggcttc gctcatggaa taagccattt
     3061 tagcaggatg ggccatgacc caagaatggc ccttcaattc gctgctgggc ttgtcacttt
     3121 tggccgtccc cctgacgcct aaggcagagc cgacgaccgg atcttgtact cacgtaaagt
     3181 ggcgccgtcg cttgtgcgcc cgcttgccat agcgggtgga tcacttagca gcttggcact
     3241 cgtcagatgt gccaccaggc agatatcagc gatggttagt cgatcgccgt agcagaagcg
     3301 tccggtgcgt ggtcatgcgc cagcacgtct tcgattgcac gg
//
LOCUS       DATLJT010000307         2910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3463488,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000307 DATLJT010000000
VERSION     DATLJT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3463488"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VM910_06985"
     CDS             complement(<1..240)
                     /locus_tag="VM910_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62323.1"
                     /translation="MTRREFITLIGGAAVAWPLAARAQQADRVRRVGVLGPGPDNPVS
                     GPGYQVFVSELRKLGFTEGQNVVVDYRRTDEGLLKA"
     gene            complement(237..1226)
                     /locus_tag="VM910_06990"
     CDS             complement(237..1226)
                     /locus_tag="VM910_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62324.1"
                     /translation="MASYIGRRKFLATLGGAAAWPLAARAQQPGKLPTIGFLGSGTLT
                     AHGHWVDAFLQRLRVLGWIEGRNLVIEYRWAEGSRDRAAVFAAEFVRLKVDIIVTYAV
                     PMVLVAKQATSTIPIVFAAAADPVGTGLVASLARPGGNVTGLSAQHTELAGKRLALLR
                     EIVPSLRRLAVMANVDNPASERELGEVQAVAGTLGLEVARLEIRREEDIARAFEELKG
                     RAEALYVCADTLLFAHRIRINTLALAARLPTMLSNRENVAAGGLAHYGANFPDLFRRA
                     GDYVDKILRGAKPGDIPVEQPTRFDLVVNLTAARALGLTIPGSFLWRADEVIE"
     gene            1487..1930
                     /locus_tag="VM910_06995"
     CDS             1487..1930
                     /locus_tag="VM910_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62325.1"
                     /translation="MDKSIVNEVVSALDAVKSSGQPGFWILLSILIVLVLVIAAVMYQ
                     RIVIKRFQTASKATQENFETLAADLRKRALEVKKQDEDLRKRELEVKERKQQQVLKRS
                     QRALKGSVSHTMLRLDFKGMTSVVPDSSKADLYRKPDEGGADAAP"
     gene            2023..2844
                     /locus_tag="VM910_07000"
     CDS             2023..2844
                     /locus_tag="VM910_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI62326.1"
                     /translation="MAERTADVQAHWTRPGVLARIDAALAELGQDPQILTPEILATVE
                     HLHSGGLATTREQAKRIALTEDSRILDVGCGIGGPARYLARTYGCRVDGIDLTPELIE
                     TGRVLTERCGLADRVVLQIGNALDLPYSDQTFDVVWCQNVTMNIADKDGLFASIYRVL
                     KSGGLFTSTEYSVGPGGDIIFPVPWAYDASISFLDREDVMRARYHTAGFRILECVNYS
                     NTVIEHYERTLSAPPKLTSRLIFGDDTPERQRNSQRNLIERRTIYWMITAERPKL"
BASE COUNT          619 a          859 c          888 g          544 t
ORIGIN      
        1 cgccttcagc agtccctcgt cggtgcggcg atagtcgacg acgacattct ggccttcggt
       61 gaacccaagc ttgcgcagct cggatacgaa gacttgatag cccggtcccg aaaccggatt
      121 gtccgggcct ggacccagaa caccgacgcg ccgcactcgg tccgcctgct gtgcccgcgc
      181 cgcgagcggc cacgcgactg ccgcgccgcc gatgagcgtg atgaactcgc ggcgcgtcat
      241 tcgattacct cgtcggcgcg ccacaggaac gatcccggga tcgtcaggcc aagcgccctg
      301 gcggcggtca gattcacgac gagatcgaac ctggtcggct gctcgaccgg gatgtcgccc
      361 ggcttcgcgc cacgcagaat cttatcgaca tagtcaccgg cgcggcggaa caggtcggga
      421 aaatttgctc catagtgtgc caggcctccg gctgcgacgt tctctcgatt actgagcatc
      481 gtcggcagtc gcgcggcgag tgccaaggtg ttgatacgaa tccggtgggc aaataggagc
      541 gtgtcggcac agacgtaaag tgcttcggcg cggcccttaa gctcctcgaa ggcgcgcgca
      601 atgtcctcct ctcgccggat ttcgagtctg gcaacttcga gaccgagcgt gcctgccacc
      661 gcctgaacct cgcccagttc ccgttcggat gcgggattgt ccacattggc catgaccgcc
      721 agccggcgga gactcggaac aatctcgcgc aaaagtgcga ggcgcttgcc agcaagctcg
      781 gtgtgctggg cggacagtcc ggtaacgttg ccgcccggcc gcgccaggga ggcgacgaga
      841 ccggtaccga ccgggtccgc cgccgccgcg aagacgatcg ggatggtcga tgtcgcctgc
      901 tttgccacga gaaccattgg gaccgcatat gtgacaataa tatcgacttt gagtcggacg
      961 aactcggccg caaacacggc ggcgcgatcg cggcttccct ccgcccagcg atactcgatc
     1021 acgaggttgc gaccttcgat ccagcccagt acgcgcagcc gctgcaaaaa agcgtcgacc
     1081 caatggccat gggccgtaag cgtgcccgag cccaagaacc cgatggtcgg taacttcccc
     1141 ggctgctgcg cgcgcgccgc gagcggccac gcggccgcgc cgccgagcgt ggctaagaat
     1201 tttcgtcgtc cgatgtagct tgccatgtga ccctccggtt gggggtcatt tttatgcaat
     1261 gggggatgat accacgcttc catcgcgcgg tctgtactga tgccgatggg caagtcgagt
     1321 gaagcctgcg aatgaccgct tgggttcaat ggcggtaagg ttgccaaatt ggcaaagagg
     1381 ccattcgctt cgtcgcgcga ttcaaagccg ctatgcgccg caatccgatt gagtagatta
     1441 gttcaggacg aaatgatgag tcgccgaggc cctaaagggg aactcgatgg ataaatcaat
     1501 tgtaaacgag gttgtgagcg cgctagacgc cgtcaagagc tccgggcagc ctggcttctg
     1561 gatattactc agtatcttga tcgttttagt gcttgtgata gctgcggtaa tgtaccagcg
     1621 catcgtaatt aaacggttcc agacggcctc aaaggcaaca caggagaact tcgaaacgct
     1681 agcggcagat ctcagaaaac gtgcattgga ggtcaagaag caggacgaag acctgaggaa
     1741 acgagagctg gaggtgaagg agcgcaagca acaacaggtg ctgaagagat cacaacgggc
     1801 cctgaagggg agtgtcagtc acaccatgct ccggctagat ttcaaaggga tgacgtcagt
     1861 agttcctgac agttcaaaag ctgaccttta ccggaaaccc gatgaaggcg gcgccgatgc
     1921 cgccccatga ggcgggctcg gtcgtcgtta gcggcctgcc gagaatttat ataaatcgcc
     1981 aggggtggac ataatttcgt tgaatcgacg agacagagcg ccatggctga acgaacggcg
     2041 gatgttcaag cgcattggac gcgacctggg gtgcttgcgc ggatcgacgc cgcactggct
     2101 gaactgggac aagacccaca gattctgacc cctgaaatcc ttgcgactgt cgagcatctc
     2161 cattctggag gcttggccac cactcgggag caggctaagc ggattgcact tacggaggac
     2221 agccggattc tcgacgttgg ctgcggcata ggcgggccgg cgcggtacct cgcgcgtacg
     2281 tatggatgcc gggtcgacgg catcgacctg acgccggagc tgatcgagac gggacgagtg
     2341 ctgaccgagc gctgcgggct tgccgaccgg gtcgtgctgc agatcggcaa tgcgctcgat
     2401 ctgccttatt ccgaccagac cttcgacgtg gtttggtgcc aaaacgttac catgaatatc
     2461 gcagacaagg acgggctctt cgccagcatc taccgggtgc ttaaatccgg cggcctgttc
     2521 acatcgacag aatattcagt tgggccgggc ggcgacatca ttttcccggt gccctgggcc
     2581 tatgacgcct cgatcagttt cctcgaccgt gaagacgtga tgcgagcgcg atatcacacg
     2641 gccggcttcc gcattctcga atgtgtgaat tattcaaata ccgtgatcga gcattacgag
     2701 agaacgctga gcgcgccgcc caagctgacc agccggctca tcttcggcga cgacacgccc
     2761 gaacggcagc gcaactcaca gcgcaacctg atcgaaagac gaaccatcta ctggatgatc
     2821 actgcagagc gccctaagtt gtagccctca cgtgagttcc tcgatgaaac gtggttctcc
     2881 gcaaacctgc cattaaatcc catccggccg
//
LOCUS       DATLJT010000308         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3922720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000308 DATLJT010000000
VERSION     DATLJT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..611
                     /locus_tag="VM910_07005"
     CDS             414..611
                     /locus_tag="VM910_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62327.1"
                     /translation="MRSSLFLAMMLAASSLAEAETPLTIEQLLAQGWEIAGYASGYDN
                     RTSLILFRHPGVNALNCYELR"
     gene            complement(596..793)
                     /locus_tag="VM910_07010"
     CDS             complement(596..793)
                     /locus_tag="VM910_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62328.1"
                     /translation="MFVTMKEDVEAFVSDLGGRVTFTRVAVTPAQVAQYALPTAPPQP
                     AARGARRTFPGKSIHRPYRSS"
     gene            complement(1397..1756)
                     /locus_tag="VM910_07015"
     CDS             complement(1397..1756)
                     /locus_tag="VM910_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62329.1"
                     /translation="MSMISPLHEAASIAEEVTTRSPHERSEMRELTWVGDRRCRFARN
                     YQIEVLEAPTGRDIDTAFARLAQKPIDALLVGPGPLLDNHRIQLGTLASYHRPPAIYP
                     LRVFAEAGGLMSRRPAL"
     gene            1749..2459
                     /locus_tag="VM910_07020"
     CDS             1749..2459
                     /locus_tag="VM910_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI62330.1"
                     /translation="MLTLYSYPELFGVADNNGYGLKVFAFLRLAGLPFRHEHIFDASS
                     APRGQLPYIVDDGETIGDSDTIIAHLIAKYRLTIDAKLTAAERDIDHLITRMLDDLYW
                     VMSYSRWKHERFWPLFRDALMRTHPSLTPEGLDKARKYNAQRYYFQGIGRYAPDAAYR
                     RGVADLQVLVDLLPASGYLHGETPTSIDAGIYGFIANIHFYDIDTPLKQFVSSHANLV
                     RHCDAIHAAVTAQPQLAR"
BASE COUNT          621 a          987 c          971 g          604 t
ORIGIN      
        1 gctcatgccg ctgctcgacc tggtgcacca ataataacgg tagaagaacc caatccgaag
       61 cgacggataa cgccgcctgt aacggttcgc gttactttca ggccacagga tggtgccacc
      121 attgatccaa gaacgttccg tgccacgtac ggatggcttg gaatcgatat tacccgccac
      181 atagttgcaa atgctaaact cagcgagtcg gggctagtgg ccaagaacgc tagtgtccca
      241 gccgggtctt atagagtgac gctgcgagta gcagataaca ggcatcgagt cggtacgcgc
      301 acggttgaat tcactgtgca taaacggtga tcacctggtc aacaagatca gacgcatatg
      361 taagaacggt gcgggttcgc gagagtctgt ctttgccgac catcggaaag tgcatgcgtt
      421 caagcctgtt cttggcaatg atgctggcgg catccagctt ggcggaagcg gagacaccgc
      481 tgacgatcga gcaactgctc gcgcaaggat gggagatcgc gggctatgcg agcggctatg
      541 ataatcgtac ttcgctgatc ctgttccgcc acccgggcgt aaacgcgctc aattgctacg
      601 agctgcggta aggccggtgg atactcttcc cgggaaaggt ccgcctagca cctcgtgccg
      661 cgggctgcgg cggcgcggtc ggcagggcgt actgcgcgac ctgggccggc gtgaccgcga
      721 cgcgggtgaa ggtcaccctg cctccgaggt cgctgacgaa cgcctcgaca tcctctttca
      781 tggtcacgaa catgtggccg ccggacggat cgtggtctcc tacgtggagg acctcgaacg
      841 gcctgtcgct cttcgagact tcaacggcga agccgtgctt gaacgtgagg ctcgcccgac
      901 gcgacgaccc ggatgccggt cgggagcggg gcaggcggcg ggccgacccc ggcagagatc
      961 ccgaccggct ccacgcagcg agcatccagg cgcagaactc tatgcaacag ctggcccggg
     1021 tggcgctcgc cgacagcttc ttggaccgcg cgggcgagca agtcacggtg ccacttagtc
     1081 gcaagcgcgg cgtaatcggc cttgggccag gtttcaaact tatggggggc cttcgatgag
     1141 ggacagcgcc caagtcactt cccccggtgc gatcaacagc atgatcccgg ctatgagcac
     1201 cagacccacg agacagaata ggagcacatg ctcgcgcatg tcggcgccta tagcgcttac
     1261 gccacccccg ccgtgtgaac cagttcacat gcgctggcgc gccaatgcgt cgcactcgcg
     1321 cccattgtgc cgccaacccg atttcgcccg gccaagccgg gggttgggcg cctataaacg
     1381 ggcaaaaacc caatgattac aatgctggcc ggcggctcat cagaccgccg gcttcggcaa
     1441 agacacgcaa gggatagatc gcaggcggcc tgtggtaaga cgccagcgta ccgagttgga
     1501 tgcgatggtt gtcgagcaac gggccgggac ctaccaacag cgcatcaatc ggcttttggg
     1561 caagcctagc aaaggccgta tcaatgtcac ggccagtggg ggcttccagg acttcgattt
     1621 ggtaattccg agcgaagcga catctgcggt caccaaccca tgtcagctcc cgcatttcgc
     1681 tgcgctcatg cgggctacgg gtcgttactt cctctgctat gctcgcggcc tcgtgcagtg
     1741 gcgatatcat gctcaccctc tactcgtacc ccgagctgtt cggcgtcgcg gacaacaacg
     1801 gctacggcct gaaggtgttc gcgttcctgc ggctcgccgg cttgccgttt cggcatgagc
     1861 acatcttcga cgcctcgtcg gcgccgcgcg gtcagttgcc ctatatcgtc gacgacggcg
     1921 agacgattgg cgacagcgat acgatcatcg cccacctcat cgccaagtat cggctgacga
     1981 tcgacgcgaa actgaccgcc gcggagcgcg atatcgacca cctgatcacc cgcatgctcg
     2041 acgacctcta ttgggtgatg tcgtactcgc gctggaagca cgagcggttc tggccgctgt
     2101 tccgcgacgc gctgatgcgc acgcatccga gcctcacgcc tgaaggtctg gacaaggcgc
     2161 ggaagtacaa cgcccagcgc tactattttc agggcatcgg acgctacgct cccgatgccg
     2221 cctaccggcg cggcgttgcg gatttgcagg tgctggtgga cctgttgccg gcgagcggct
     2281 acctgcacgg cgagacgccg accagcatcg acgccggcat ctatggcttc atcgccaata
     2341 tccattttta cgacatcgat acgccgctca agcagttcgt ctcgtcccac gccaatctcg
     2401 tgcgccattg cgatgcgatc catgcggccg tgaccgcgca gccgcagctt gcccggtagt
     2461 attcaaacac tctctgctgt tcgtccccgc gagggcccgc ggccctggat cagaagtgcc
     2521 cattcggcgg agctaactct caacgtgcca atggcacggt cttcctactc gggcctcagc
     2581 gctgtcgaga gagagcgcga cacttttttg cgacatttga agtgagaaag gtgtcgccgg
     2641 gctggccggg ctggccttgc gagaagtccc cttctggtaa ccgcccaccc acggcgagcg
     2701 ccgccgccgg ggctttgatg gcctcccgag cccatcgcga ctagctgacg gcggactgtg
     2761 ctgaaggaag gctaaggcgt catcacggag tgctggaatg tctctcagcc cctgatcggc
     2821 catcttcatg atgcgcttgg ccaggatcgt ttgcgtcgcg agcgatttgg gtgctgcgtg
     2881 aggtcgctgc caagaccctg catcgaacgc atcgcaaatc gcgcgtgtct tgctaaactc
     2941 aaacgccagc ggattttcaa ccatgtcgca aacgcctcac ccgacatgct tcggtgcgag
     3001 agcggaggga cgctcaaaag ggtgctgcga tgcagtggat tgaaggcccg gtcgctttgc
     3061 gatggcggct cgatcactcg gaagctacga ccgccacagt cgtaagtcga gctacagttt
     3121 tgtgacggtc cgcaaattgc tcatcggctg cggtgccaga atgccgctga cgggtgcaga
     3181 ata
//
LOCUS       DATLJT010000309         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1395493,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000309 DATLJT010000000
VERSION     DATLJT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395493"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VM910_07025"
     CDS             <1..1078
                     /locus_tag="VM910_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI62331.1"
                     /translation="EATAATRLRIVDCDIHPSLHSRADLNHFLPKRWQEHMKTYGDHL
                     RTPYIGTTPYPRSSPLICRRDAWPPTGGPPGSDLAFMRKQHLDPLDVEFGILQVLDLF
                     IFSQQNLEFGAAIQRAINEWQLAFWSHRDPRLKASILVSQDDTETALAEIDRCAKTGE
                     YIQVNISPRANEPLGRRRYWPIYQRAEALGLPLGIHVGGYGGHAPTGGGWPSYYCEEH
                     QSNAHTMAAQLASLVIEGVPERFPKLKIVFIEGGFGWIASAMWRMDRHFERFRSEVPH
                     LKRKPSEYVKEQFWFTTQPIDEPDEARHLRQLIEWVGIDRLLFSSDYPHWDFDDPRFA
                     FKTPLTDAERKKIFSTNARGVYKI"
     gene            1078..1503
                     /locus_tag="VM910_07030"
     CDS             1078..1503
                     /locus_tag="VM910_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVI62332.1"
                     /translation="MARHVVARTSEIPPGGNKVVGIDGRDIVVFHVNGEFFALLNRCP
                     HEGAPLDKAACVARLTSPEPGVYQRSRVGELLRCAWHGWEFDMRNGQSWFDPKRFKVR
                     SFPVAVESGEELAAEMKKGPYVAETLPVVVEDSYVIVET"
     gene            1661..2788
                     /locus_tag="VM910_07035"
     CDS             1661..2788
                     /locus_tag="VM910_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HVI62333.1"
                     /translation="MKFPYLFSPLVVGPCQLKHRLVMAPLTRMRALKPSHAPRPLNAE
                     YYAQRATEGGLIIAEASPVTTTAFGNPGVPGIWSDQQVEGWRKVVDAVHAKGAFIFLQ
                     LWHVGRVSHPSFQPGGALPVAPSAVAISTEFKAMTADGRVVPYETPRALETDEIPGIV
                     AAFQQGARNALAAGFDGVEIHGANGYLLEQFLQSRSNRRTDRYGGSIEDRARLLLEIV
                     QGAIEVWSANRVGVRLSPYGIANDSGEPEPMPLYTHVVQALNPLGLAYLHFIEPRSSG
                     AGRAEVNWQNVPSAMVLFRPIWKGALIAAGGFVGETAEAAIAEGHADAIAFGRIFISN
                     PDLLRRLQNGFPLTPYNRATFYGGEEAGYTDYPVYDEMAPA"
     gene            2896..3399
                     /locus_tag="VM910_07040"
     CDS             2896..3399
                     /locus_tag="VM910_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI62334.1"
                     /translation="MARKAAAGGIAKGQCLCGRVSFEIDVPARWAWHDHSSASRRAHG
                     AAYATYVGSWRKRLRITAGKAAISRYEDTSAKTARSFCAQCGTPLFYERARSPHMVNI
                     PRALFSGRTGRQPLYHIAIEELQEWAYTGEPLVPLKGFPGVVWQRSKKKRRAAGDDPF
                     ELSRDDV"
BASE COUNT          619 a         1136 c         1127 g          678 t
ORIGIN      
        1 ggaagcgacc gccgcaacgc ggctgcgcat cgtcgattgc gacatccatc cgagcctgca
       61 ttcgcgcgcc gacctcaatc atttcctgcc caaacgctgg caggagcata tgaagaccta
      121 cggcgaccat ctgcgcacgc cctatatcgg caccacgccc tatccgcgct cctcgccttt
      181 gatctgccgc cgcgacgcct ggccgccgac gggcgggccg cccggctccg atctcgcctt
      241 catgcgcaag cagcatctcg atccgctcga cgtcgagttc ggcattctgc aggtgctcga
      301 cctcttcatc ttctcgcagc agaatctgga atttggagcc gcgatccagc gcgccatcaa
      361 tgaatggcag cttgcgttct ggtcgcatcg cgatccccgg ctgaaagcct cgattctggt
      421 cagccaggac gatacggaga ccgcgctcgc ggagatcgac cgttgcgcga agaccggcga
      481 atatatccag gtcaacatct cgccgcgcgc caacgagccg cttggccgcc gccgttactg
      541 gccgatttac caacgcgccg aggctctggg acttccgctc ggcatccatg tcggtggtta
      601 tggtgggcac gctccgaccg gcggcggctg gccgtcctat tattgcgagg agcatcagtc
      661 gaatgcgcac acgatggcgg cgcagctcgc aagcctcgtg atcgaaggcg tgccggagcg
      721 cttcccgaag ttgaagattg tcttcatcga gggcggtttt ggctggatcg cctccgcgat
      781 gtggcggatg gaccggcatt tcgaacgctt ccgcagcgaa gtgccgcatc tcaagcgcaa
      841 gccttcggaa tatgtgaagg agcaattctg gttcacgacg cagccgatcg acgaacccga
      901 cgaggccagg catctgcggc aactgatcga atgggtcggt atcgaccggc tgttgttctc
      961 gtcggactat ccgcattggg atttcgacga cccgcgcttt gccttcaaga cgccgctcac
     1021 ggacgccgag cggaagaaga tcttcagcac caacgcgcgc ggggtttaca agatctgatg
     1081 gctcgtcacg tcgtcgcccg cacatcggaa atcccgcccg gcggcaacaa ggtggtcggg
     1141 atcgacggcc gcgacattgt cgtgttccat gtcaacggcg agttcttcgc gcttctcaat
     1201 cgctgcccgc atgagggcgc gccgctcgac aaggcggcgt gcgtggcgcg tctgacctcg
     1261 ccggaacccg gcgtttacca gcgctcgcgc gtgggcgaac tgttgcgctg tgcgtggcac
     1321 ggctgggagt tcgacatgcg caacggtcag tcctggttcg atccgaagcg cttcaaggtc
     1381 cgctcgtttc cggttgcggt cgaaagcggc gaggagcttg ctgccgaaat gaaaaaaggg
     1441 ccatacgtcg ccgagacttt gccggtcgtt gtcgaggatt cctacgtcat cgtcgagacg
     1501 tgaagcgccg gcggtaagcg gctgtgccgg agggcatctt gctccacgtt tttggataag
     1561 gggcattcag gagtggcggc tatccgcccg ctgctccttc ttttatgatg gcgatcagat
     1621 caatcgttga tccctcaaca tattcctttt cggggcagca atgaaatttc cgtatctttt
     1681 ttcgccgctc gtcgtgggtc cctgtcagct caagcatcgc ctggtgatgg cgccgctcac
     1741 ccgcatgcgg gccttaaagc cttcgcacgc accgcgcccg ctcaacgccg aatattatgc
     1801 gcagcgcgcc accgagggag gactgatcat tgccgaagcc tcgccggtta cgacgactgc
     1861 cttcggcaat cccggcgtgc ctggcatctg gtcggaccag caggtcgaag gctggcgcaa
     1921 ggtcgttgac gccgtccacg ccaagggcgc cttcattttt ctgcagctct ggcatgtcgg
     1981 gcgcgtctcg cacccctcat tccagcccgg cggcgcgttg ccggtcgcgc cctcggcggt
     2041 ggcgatctcg accgagttca aggccatgac ggcggacggc agggtggtcc cctacgagac
     2101 gccgcgggcg ctcgagacgg atgaaattcc cggcatcgtc gcagccttcc agcaaggcgc
     2161 gcgcaatgcg ctcgccgccg gcttcgatgg cgtcgagatt cacggcgcca acggctatct
     2221 gctcgagcaa ttcctgcaat cgcgcagcaa ccggcgcacc gaccgctatg gcgggtccat
     2281 cgaggaccgc gcgcggctgc tgctggagat cgtgcagggc gcgatcgagg tctggagcgc
     2341 aaaccgcgtc ggcgtcaggc tctcgcctta tggcatcgcc aacgacagcg gcgagccgga
     2401 gcccatgccg ctctatacgc atgtcgtgca ggcgctcaat ccgctcggcc tcgcctattt
     2461 gcacttcatc gagccgcgat cgagcggcgc gggccgcgcc gaggtcaatt ggcagaacgt
     2521 gccgtccgca atggtgctgt tccgcccgat ctggaagggc gctctgatcg cggccggcgg
     2581 atttgttggc gaaaccgcgg aagcggcgat tgccgagggt catgcggatg ccatcgcctt
     2641 cggccgaatc ttcatctcca atccggattt gctgcgccgc ctgcagaacg gttttcctct
     2701 gacgccctat aatcgcgcga ccttctatgg cggggaggaa gcgggctaca cggattatcc
     2761 ggtttacgac gagatggcgc cagcctagcg tcgtcattgc gagcgctgcg agcgaatgag
     2821 ttcgtgtttc cgggtctgtc ctttcgaact ttgtcgcttt cgctcctcgc aacgacgaag
     2881 gagagggaag gcgcgatggc aaggaaagcg gcagcaggcg gcatcgcaaa aggccaatgc
     2941 ctgtgcggca gggtttcatt cgagatcgac gtgccggcgc gctgggcctg gcatgaccat
     3001 tcgtcggcaa gccgccgcgc gcatggcgca gcctacgcca cctatgtcgg tagctggcgc
     3061 aagcgccttc gcattactgc tggcaaggcc gcgatctcgc gctacgagga cacgagcgcg
     3121 aaaactgcgc gcagcttctg cgcgcagtgc ggcacgccgt tgttttacga gcgggcgcgc
     3181 tcgccgcata tggtcaatat cccccgcgcg ctgttctcgg gccgcaccgg gcgtcaaccg
     3241 ctctatcaca tcgcaatcga ggaacttcag gagtgggcct ataccggcga gccgctggtg
     3301 ccgctcaagg gctttccggg cgtcgtatgg caacgctcga agaagaagcg gcgcgcggcg
     3361 ggcgacgacc cgttcgaact cagccgcgac gatgtctgat ttgcggaagg tgcgtgcgaa
     3421 gcggcggcct tgtcgtggcc cgccgcgcaa ggcagccatg atcctcctcc attgatctcg
     3481 gcatcgggat cagcatgatg ccgtaaagcc cgcgccaccg gcggcgggga gggaggagac
     3541 atgaagaacc gtatcaccgg
//
LOCUS       DATLJT010000310         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1854997,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000310 DATLJT010000000
VERSION     DATLJT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854997"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VM910_07045"
     CDS             <1..1121
                     /locus_tag="VM910_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVI62335.1"
                     /translation="GRIFYNQAQMSAAGIPQIAIVMGSCTAGGAYVPAMSDESIIVRN
                     QGTIFLGGPPLVKAATGEVVTAEELGGADVHSRQSGVTDHYAQNDAHAIGIARRIVAT
                     LKPPTRAPLNMREVREPLFAPEEIYGVVPVDGRKPFDVRDIIARLVDGSEFGEFKKLY
                     GQTLVCGFVHIWGYPVGIIANNGILFSESSLKGAHFIELCCQRQIPLVFLQNITGFMV
                     GRKYEAGGIARDGAKLVTAVATASVPKFTVVIGGSYGAGNYGMCGRAYAPRFLFMWPN
                     ARISVMGGEQASMVLSQVRRDNIEAKGDRWSAEEEQEFRAPIRAQYESQGNPYYATAR
                     LWDDGVIDPADTRLVLGLGLSAASNAPIEPTKFGLFRM"
     gene            1124..>2160
                     /locus_tag="VM910_07050"
     CDS             1124..>2160
                     /locus_tag="VM910_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVI62336.1"
                     /translation="MTRAKMYRRFRTLLVANRGEIACRVIRTARAMGLRTVAVYSEAD
                     RGAMHVGMADDAVLLGPARARDSYLNVERVLDAARRTAAEAVHPGYGFLSENAEFAQA
                     CSTAGLVFVGPTAEMMTAMGSKSGSKALMEKAGVPLVPGYHGEAQDDATLTRAAEKIG
                     FPVLVKASAGGGGRGMRIVRSAADLAEAIVSAKREAKAAFGDDRLLVEKYVDNPRHIE
                     VQVIGDSHGNLVSLFERECTLQRRHQKVIEEAPSPTLNAGQRETVCGAARKAAAAVNY
                     VGAGTIEFVSDGKEVFFIEMNTRLQVEHPVTELITGIDLVEWQLRVAFGERLPLAQDQ
                     ITMRGHAVEARV"
BASE COUNT          386 a          643 c          738 g          393 t
ORIGIN      
        1 tcggccgcat cttctacaac caggcgcaga tgtcggcggc cggcattccc cagatcgcaa
       61 tcgtgatggg ctcctgcacc gcgggtggtg cctacgtgcc ggcgatgtcg gacgaaagca
      121 tcatcgtgcg caaccagggc acgattttcc tgggcgggcc gccgctggtg aaggctgcca
      181 ccggcgaggt ggtcacggcg gaagaacttg gcggcgccga cgtgcattcg cggcaatccg
      241 gtgtcaccga tcactacgcg cagaacgatg cgcatgcgat agggattgcc cgccgcatcg
      301 ttgccacgct gaaaccgccg acccgggctc ccttgaacat gcgcgaggtg cgcgagccgc
      361 tgtttgcgcc ggaggagatt tacggcgtgg tgccggtcga tggacgcaag ccgttcgacg
      421 tgcgcgacat catcgcgcgt ctggtcgacg gttcggaatt cggtgagttc aaaaagctct
      481 acggccagac gctggtctgc ggcttcgtcc atatctgggg ctacccggtc ggtatcatcg
      541 ccaacaacgg catcctgttc agcgagagct cgctgaaggg tgcgcatttc atcgagctgt
      601 gctgccaacg tcagattccg ctggtgtttc tgcagaacat cacgggcttc atggtcggca
      661 gaaaatacga ggccggcggc attgcccgtg atggcgcgaa gctcgtgacg gcggttgcga
      721 cagcatcagt gccgaaattt accgtcgtga tcggcggttc ctatggggcc ggcaattacg
      781 gcatgtgcgg gcgcgcctac gctccgcgct ttctctttat gtggccgaat gcccgcatct
      841 cggtgatggg cggcgagcag gcctcgatgg tgctgagcca ggttcgccgc gacaacatcg
      901 aagccaaggg cgatcgctgg tcggcggagg aggaacagga gtttcgcgcg ccgatccggg
      961 cgcagtatga aagccagggc aacccctatt atgcgaccgc gcggctgtgg gacgacggtg
     1021 tgatcgatcc cgccgacacg agattggtgc ttggtcttgg gttgtcggcc gcatcgaacg
     1081 cgccgatcga acccaccaaa tttggcctgt tcaggatgtg acaatgaccc gcgcgaaaat
     1141 gtaccggcgg tttcgcaccc tgctcgtcgc caaccgcggc gagatcgcct gtcgcgtcat
     1201 ccgcaccgca cgcgcgatgg gtcttcgcac ggtcgcggtc tattccgagg ccgatcgcgg
     1261 cgccatgcat gtcgggatgg cagatgacgc ggtgctgctg ggaccggcgc gggcacgcga
     1321 cagctatctc aacgtcgaac gcgtcctcga tgccgcgcgc cggaccgcag ccgaagccgt
     1381 gcatcccggc tacggctttc tgtcggaaaa cgccgaattc gcgcaggcct gcagcactgc
     1441 gggcctggtg ttcgtcgggc cgaccgctga aatgatgacc gcaatgggct cgaagtccgg
     1501 ctcgaaagcg ctgatggaga aggcgggcgt gccgctggtg ccgggctatc acggcgaagc
     1561 gcaggatgac gcgacgctga ccagggcggc cgagaagatc ggctttccgg tgctggtcaa
     1621 ggcttctgcc ggcggtggcg gtcgcggcat gcgcatcgtg cgttcggcgg cggatctcgc
     1681 agaagctatt gttagcgcaa aacgcgaggc caaggccgca ttcggcgacg accgcttgct
     1741 ggtcgagaaa tatgtcgaca atccacgaca tatcgaagtg caggtgatcg gcgacagcca
     1801 cggcaacctg gtctctttgt tcgagcgtga gtgcacgctg cagcgccgcc atcagaaggt
     1861 gatcgaggaa gcgccgtcgc cgaccctcaa tgccgggcag cgcgagacgg tttgcggggc
     1921 cgcgcgaaag gccgctgccg ccgtcaacta cgtgggcgcg gggacgatcg agttcgtgtc
     1981 cgacggcaag gaggtgtttt ttatcgaaat gaacacccgc ctgcaggtcg agcatcccgt
     2041 gaccgaattg atcacgggta tcgatctcgt cgagtggcag ttgcgcgtcg cctttggcga
     2101 aaggctgccg ctggcgcagg atcagatcac catgaggggt catgccgtcg aggcgcgggt
//
LOCUS       DATLJT010000311         3114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_17314,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000311 DATLJT010000000
VERSION     DATLJT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17314"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VM910_07055"
     CDS             complement(<1..361)
                     /locus_tag="VM910_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62337.1"
                     /translation="MVKTPPVGGKREIPAGRLRGSRNKLSEEVICALLRDFREHGQKA
                     IAKVRRDQPGIYLKVLALLIPRQDKLEHTSTIKTMTDEELESAIAFLHEMMAAQAVGS
                     AHLVEMKAEPAALPAPGG"
     gene            complement(751..1101)
                     /locus_tag="VM910_07060"
     CDS             complement(751..1101)
                     /locus_tag="VM910_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62338.1"
                     /translation="MASLEYVRTGKLRALGVTSATRWQTLPEVPTIGEFVPGYEVNLW
                     NGVGAPRDTPKEIVEKLNGEINAGLADPKIRARLAELGSVPMPLSPTEFGKLWADDTE
                     KWGKVIRAANIKAD"
     gene            complement(1088..1729)
                     /locus_tag="VM910_07065"
     CDS             complement(1088..1729)
                     /locus_tag="VM910_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62339.1"
                     /translation="MNLPRRQLLYLAAGAAALPVVSRIARAQAYPSRPVRWIVGFSAG
                     GTTDILARLMAQWLSERVGQQFIIENRPGAGTNIALQAAVNSRPDGYTLVSVTSSNAS
                     NATLYESLPFNLQRDIIPVGALSKNALVLEVNPSISIKSVPEFIAHAKTNPGKITVAS
                     FGVGSTSHLAQELFKAMTGINVVHVPYRGDATRADRCDQRASTGHVQQCGGVP"
     gene            complement(1735..>3114)
                     /locus_tag="VM910_07070"
     CDS             complement(1735..>3114)
                     /locus_tag="VM910_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI62340.1"
                     /translation="LKETEVLGAIYIYRQEVRPFSDKQIALLASFASQAVIAIENTRL
                     LNELRESLQQQTATADVLKVISRSTFDLQVVLNTLVESAARLCESDMASIARQKGANY
                     HLVATHGYPYGFKEYMETLPMEPGRGSVTGRVLLERKPVHVIDVQADPEHTMVEARKR
                     AGFRTMLAVPLLREGTPIGVLHVLRTSVRPFTDKQIELVETFADQAVIAIENVRLFDE
                     IQDKSKQLAEASERKSQFLASMSHELRTPLNAIIGLTEMMVTNTPRFGTEKALEPLRR
                     VNAAGTHLLSLINEILDLSKIEAGKLELNPEPVNLARLIDEVIGTAGQLAEKNKNRLV
                     IEAQENLGVLIADSMRLKQILLNLLSNACKFTKEGEVALRVRKAADGRDWIELAVADS
                     GIGMTAEQQAKLFQDFTQADSLTARRYGGTGLGLAISRKLARMMGGDVTVASEPGKGS
                     VFTVRLPGG"
BASE COUNT          581 a          954 c          916 g          663 t
ORIGIN      
        1 gccctccggg agccgggagc gcggcaggtt ctgccttcat ctcaaccagg tgcgcgctcc
       61 caaccgcttg agccgccatc atttcgtgaa gaaacgcaat cgcactctct agctcttcgt
      121 ccgtcatggt tttgatcgtg ctggtatgct cgagcttgtc ttgtctcggg atcaataaag
      181 caagaacttt caagtagatc ccaggctggt cgcggcgcac tttcgcaatc gccttttgcc
      241 cgtgctcacg gaaatcgcgc aagagcgcgc aaatgacctc ctcgctcagc ttgttacgcg
      301 agccgcgcag tcttccagca ggaatttcac gcttcccccc taccgggggg gttttcacca
      361 cggcggcgta tctctctggc cctgctccag ccgacgccaa gtgactcgac acgggcggca
      421 accaggtggt ggcgaacgca tgggaggtta agcggtagct ttcagtagcc gggcgcgaca
      481 gggatcagac taggcacacg ccattcgcgg aacggtgagg tttctatgag gtcgctcggt
      541 ggagacgagc ctcaaatacg cagatgagcg cgatcttata gaaaccttcg ttgaacgaag
      601 ccgctggcac tgtcgagcag cgagggagac tttcagctcg gacgaatttc gacccgcgac
      661 gacggtgctc gcgagtacaa cttgagcgtt acggcacgaa tgttgctaat aacggaggtt
      721 atggaatatc gacagcggat cggcctcata tcagtccgcc ttgatgttgg ccgcccggat
      781 aaccttcccc cacttctcgg tatcgtcggc ccagagtttc ccgaactccg ttggcgacag
      841 cggcatgggg acgctgccca actcggcaag tcgtgccctg atcttcggat cggcaaggcc
      901 ggcattgatc tcgccattga gcttctcgac gatctccttg ggcgtgtctc tcggcgcacc
      961 gaccccgttc caaagattta cctcgtagcc tggcacaaac tcgccgatgg tcggcacctc
     1021 gggcagcgtt tgccagcgcg tcgccgaggt cacgccgagc gcacgcagct tgccggttct
     1081 gacatattca agggacgcca ccacattgct gaacgtggcc tgtacttgcc cgctgatcgc
     1141 atcggtcagc gcgggtggca tcgccacgat aaggcacatg caccacgttg ataccggtca
     1201 ttgctttgaa cagttcttgc gccagatggc tggtcgaacc gacgccaaat gatgcaacgg
     1261 tgattttccc cgggttggtc ttcgcatgcg caataaactc tggcaccgat ttgatcgaga
     1321 tcgacgggtt cacctccaag accagcgcat tcttggatag ggcgccaacc ggaatgatgt
     1381 cgcgttgcag gttgaatggg agcgactcat agagcgtcgc gttgctcgca ttggagctcg
     1441 tcacggaaac gagggtgtag ccgtcgggtc gcgaattgac ggctgcctgc aaggcgatgt
     1501 tggtgccggc tcctggcctg ttctcgatga tgaattgctg accgacccgc tccgacagcc
     1561 attgtgccat cagacgtgcg agaatgtcgg tcgtgccgcc cgccgaaaaa cccacgatcc
     1621 agcgcacagg ccgcgacggg taggcttgcg ccctcgcgat gcgcgagacc accggcagtg
     1681 cggcagcacc cgccgcaaga taaagaagtt gacgacgcgg caggttcatt gcgtttagcc
     1741 ccccggcagc cgcaccgtaa ataccgagcc cttgcccggc tcgctcgcca ccgtcacgtc
     1801 gccgcccatc atgcgtgcga gcttgcgcga gatggcaagg ccgagccccg tcccgccgta
     1861 gcggcgcgcc gtcaaggaat cggcctgcgt gaaatcttgg aacagctttg cctgctgctc
     1921 cgccgtcatg ccgatgccac tatccgcgac ggcaagctcg atccagtcgc gtccgtccgc
     1981 cgccttgcgc acccgcagcg cgacctcgcc ctccttcgtg aacttgcagg cattgctcaa
     2041 gagattgagc agaatctgct tgagcctcat agagtccgcg atgagcaccc ccaggttctc
     2101 ttgcgcttcg atgacgaggc ggttcttgtt cttctcggcg agctgtcccg cggtgccgat
     2161 gacctcatcg ataagtctgg ccaaattcac gggctcggga ttgagttcga gcttgccggc
     2221 ttcgattttc gagaggtcga gtatctcgtt gatgaggctc agcagatgcg tcccggcggc
     2281 attgacacgg cgcagcggtt ccagcgcctt ttccgtgccg aagcgcggcg tattggtgac
     2341 catcatttcg gttaggccga tgatcgcgtt gagcggcgtg cgcagctcgt ggctcatgct
     2401 ggcgaggaat tgcgacttgc gttcgctcgc ctccgccagc tgcttgctct tgtcctggat
     2461 ttcgtcgaac agccgcacgt tctcgatcgc gatcaccgcc tggtcggcga aggtctcgac
     2521 cagctcgatc tgcttgtcgg tgaagggccg cacgcttgtg cgtaacacgt ggagaacgcc
     2581 aataggggtc ccctcccgca agagcggcac tgcaagcatt gtgcgaaagc cggctctctt
     2641 tcgagcttca accatcgtgt gttccggatc ggcttgaaca tcgatgacat gaaccggttt
     2701 ccgctcaagt agaactcggc cagttaccga cccgcggccc ggctccatcg gcaaggtttc
     2761 catgtattct ttgaagccgt acgggtaccc atgcgtcgct acaaggtgat agttcgcgcc
     2821 cttctggcga gcgatcgacg ccatgtccga ctcacaaagt cgggcagctg attcgacaag
     2881 cgtattcaac accacttgca aatcgaaggt cgagcggctg atgaccttga gcacgtcggc
     2941 ggtggcggtc tgctgctgca gcgactcccg cagctcattg agcaggcgtg tgttctcgat
     3001 ggcgatgacg gcttggcttg caaaactcgc gagcagcgcg atttgtttat cgctgaacgg
     3061 ccggacctcc tggcgataga tgtagattgc accaagcacc tcggtttctt tcag
//
LOCUS       DATLJT010000312         2528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3234052,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000312 DATLJT010000000
VERSION     DATLJT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234052"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VM910_07075"
     CDS             <1..554
                     /locus_tag="VM910_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HVI62341.1"
                     /translation="LRFGLPRPPVLAHRLDKDTSGCLVLGRHRKATASLGLLFKHGKI
                     DKIYWAVVEGGPAGDEGTIELPLGRKNAERGWWQKPDPEGQPAVTNWKVIGRGESLSW
                     LAMEPVTGRTHQLRVHAAAMGWPIVGDNIYGNGPRFGEPRLHLHSREIGIPISKNRDP
                     VRVIAPAPPHMHERLRACGWNGE"
     gene            complement(669..1922)
                     /gene="mtaB"
                     /locus_tag="VM910_07080"
     CDS             complement(669..1922)
                     /gene="mtaB"
                     /locus_tag="VM910_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HVI62342.1"
                     /translation="MSVEVVTFGCRLNAFESEVIAREAKQAGLERTIVINSCAVTNEA
                     VAQARQSIRRLKRERPEARIIVTGCAAQTQAKMFAEMREVDRVIGNDDKMRSDAWRAT
                     RAALDVASEDKVAVSDIMSVREMAPHLVDGVQRGLPRVFVQVQNGCDHRCTFCIIPFG
                     RGNSRSVPIGAVVDQVRALVERGHAEIVLTGVDLTSYGADLPGAPKLGMLVKQILRHV
                     PDLKRLRISSIDSVEADSTLLDALASDDRLMPHLHLSLQSGDDLILKRMKRRHSRADA
                     IEFCRQVRRLRPGIALGADIIAGFPTETYEMFARSLDLVDECGLTFLHVFPYSPRPGT
                     PAARMPQVAGEAIKERAKRLRQAGEGALQRRLASEIGVTREVLIESDKLGRTEHYLPV
                     AIAGETPGTVRRLVVADHDGSRLTV"
     gene            complement(1919..>2528)
                     /gene="dapF"
                     /locus_tag="VM910_07085"
     CDS             complement(1919..>2528)
                     /gene="dapF"
                     /locus_tag="VM910_07085"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVI62343.1"
                     /translation="RRLFEKTGQTAATFETNAGLLNCWQGPAPDLYTVDMGAPKFGWQ
                     DIPLAEEFRDTRYIELQIGPIDAPILHSPSAVSMGNPHAIFWVDDVNAYDLARFGPLL
                     ENHPIFPERANITLAHIVDRDHITIRTWERGAGLTKACGSAACATAVAAARLKRANRC
                     VEITLPGGKLAIEWREGDDHVLMTGTAAFEYEGQFDPTLFAA"
BASE COUNT          452 a          828 c          815 g          433 t
ORIGIN      
        1 cgctacgctt cggcctgccg cggccgccgg tgcttgccca ccggctggac aaggacacct
       61 ccggctgcct cgtgctcggc cgccatcgca aggcaacggc ttcgctcggc ctgttgttca
      121 aacacggcaa gatcgacaag atctactggg ccgtcgtcga aggcggaccc gccggggacg
      181 aaggcacaat cgagctgccg ctcgggcgca agaatgccga gcgcggctgg tggcagaagc
      241 cggacccgga gggacagccg gcggtcacca actggaaagt gatcggtcgc ggcgagagtc
      301 tctcctggct tgccatggaa cctgtcaccg gccgcacgca tcaactgcgc gtacatgccg
      361 cagcgatggg ctggccgatc gtcggcgaca acatctatgg caatggcccg cgtttcggcg
      421 agccgcgatt gcacctgcat tcccgggaga tcgggattcc gatttcgaag aacagggatc
      481 cggtgcgggt aattgcgccg gcgccgccgc atatgcatga gcggctgcgc gcatgcggct
      541 ggaacgggga gtgaggcgga gcgaagaccg ctggctcgtc gtggccgggc ctgtcccggc
      601 cattcacgtc ttgtggcaac gaaaggtaag gcatggatgc ccgggtcaag cccgggcatg
      661 acgacgttct agacggttag tcgcgaacca tcatggtcgg cgacgacaag tcgccgcacc
      721 gtgcccggcg tctcccccgc aatggcaacc ggcaaataat gctctgtccg cccgagcttg
      781 tcgctttcga tcaagacctc gcgggtcacg ccgatttctg acgcgagccg ccgctgcaac
      841 gccccttcgc ccgcttggcg cagccgtttc gcgcgctcct tgatcgcctc gcctgcgacc
      901 tgcggcatcc gcgctgccgg cgtgcccggg cgcggcgagt aaggaaaaac atggaggaag
      961 gtgaggccgc attcgtccac cagatcgagc gagcgcgcaa acatctcgta ggtttcggtc
     1021 gggaaacccg cgatgatatc ggcaccgagc gcgatacccg gacgcagccg gcgcacttgc
     1081 ctgcaaaatt cgatcgcgtc ggctcgcgag tgtcgccgct tcattcgctt gaggatcaga
     1141 tcatcgcccg actgcagcga aagatgcaga tgcggcatca gccggtcgtc gctcgcaagt
     1201 gcatcgagca gggtggaatc cgcctcgacc gaatcgatcg acgaaatcct caagcgcttc
     1261 aagtcaggca catgccgcag gatctgcttg accagcatac ccagtttcgg cgcgcccggc
     1321 aggtcggcgc cgtagctcgt gaggtcaacg ccggtcagca cgatctccgc gtggccacgc
     1381 tcgaccagcg cgcggacctg atcgaccacg gcgccgatcg gcaccgagcg cgaattgccg
     1441 cggccgaatg gaatgatgca gaaggtgcag cggtgatcgc aaccgttctg cacctgcacg
     1501 aagacccgcg gcagaccgcg ttgaacgccg tcgaccaggt gcggcgccat ctccctgacc
     1561 gacatgatgt cgctgacggc gaccttgtcc tccgaggcga catcaagcgc ggcgcgcgtc
     1621 gcgcgccagg catcgcttcg catcttgtcg tcgttgccga tgacgcgatc gacctcgcgc
     1681 atctcggcga acatctttgc ctgcgtctgc gctgcgcagc cggtcacaat gatgcgcgcc
     1741 tcgggccgct cccgtttcag cctgcggatc gattgccggg cctgcgcgac agcctcgttg
     1801 gtgaccgcac agctattgat gacgatggtc cgctcgaggc cggcttgttt cgcctcgcgc
     1861 gcgatcacct cggactcaaa cgcgttgagg cgacagccaa aggtgacgac ctcgacgctc
     1921 atgcggcaaa cagggtggga tcgaactgac cttcgtattc gaaggctgcc gttcccgtca
     1981 tcagcacgtg atcgtcgccc tcgcgccatt cgatcgcgag ctttccgccg ggaagggtga
     2041 tctccacaca gcgattggcc cgcttcagcc gggccgcggc caccgccgta gcgcaggccg
     2101 ccgagccgca agctttggtc agtcccgccc cgcgctccca ggtgcggatg gtgatgtgat
     2161 cgcgatcgac gatatgggcg agcgtgatgt tggcccgttc cgggaaaatc ggatgatttt
     2221 ccaggagcgg tccgaaacgg gcgagatcgt aggcattgac gtcgtcgacc cagaagatcg
     2281 cgtgcggatt gcccatgctg acggcggagg gcgaatgcag gatcggcgca tcgatcggac
     2341 cgatctgcag ctcgatgtag cgcgtgtcac gaaattcctc ggcaagcgga atatcctgcc
     2401 agccgaattt cggcgcgccc atgtcgacgg tgtagaggtc cggtgccggc ccctgccagc
     2461 aattcagaag ccccgcattg gtctcgaacg tcgccgcagt ctggcccgtc ttctcgaaca
     2521 ggcggcgc
//
LOCUS       DATLJT010000313         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2544762,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000313 DATLJT010000000
VERSION     DATLJT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544762"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VM910_07090"
     CDS             <1..582
                     /locus_tag="VM910_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62344.1"
                     /translation="VAALRNHLNARGGGEGLPDNDRGLLERHLARVTLTPNHLELRLR
                     ELVEPAQAHGPANTSARRPTASVTTMAVPWTSPVPAAVKGIIHVPAHNTPIKASRREA
                     LLIAIAKARQWIDDLAHGRAASFAVIARREGKVERHIRLLAPLAFVSPRIVSALLDGT
                     APADLTLTKLARALPYCWAEQERRVGTSAPFHG"
     gene            1154..1726
                     /locus_tag="VM910_07095"
     CDS             1154..1726
                     /locus_tag="VM910_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI62345.1"
                     /translation="MTTIKAHLSSVELEACYKTAVDPIGKSHFHALWLLSSGYEVEEV
                     AELLSFSPRWVRALLKRYNEGGAEALGDQRVHNGTKPTILTPEALAALKERIKMPPDD
                     GGLWTGPKIARWLARFHGVRSVHDQRGWDALIAIGYSIQQPRPRHPDAATDEDRAALK
                     KSLRRRLTRSSASIRARALKSGRWMNIASV"
     gene            1687..2217
                     /locus_tag="VM910_07100"
                     /pseudo
     CDS             1687..2217
                     /locus_tag="VM910_07100"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          508 a          813 c          803 g          488 t
ORIGIN      
        1 gttgcggcgc tgcgcaacca cctcaacgcg cgcggcggcg gggaggggtt gcccgacaat
       61 gatcgcggcc tcctcgagcg tcatctcgcg cgtgtgacat tgaccccgaa ccatctcgag
      121 cttcggctgc gcgagctcgt cgagcccgcg caagcccacg gccctgcaaa cacctcagca
      181 cggcgtccga ccgcgagcgt cacgacgatg gcggtccctt ggaccagccc tgtccccgcc
      241 gccgtcaagg gcatcatcca tgtgcccgcg cacaacacac cgatcaaagc tagccgccgc
      301 gaggcgctcc tgatcgcgat cgccaaggcc cgccaatgga tcgacgattt ggcgcatggt
      361 cgcgccgcca gtttcgccgt gatcgcccgg cgggaaggaa aggtcgagcg gcacatccgg
      421 ctgttggcac cgctcgcctt cgtctcaccc cggatcgtgt cggcactcct cgacggcacc
      481 gcgccggccg atctcaccct cacaaagctc gcccgcgcgc tgccctattg ctgggccgag
      541 caggaaaggc gagtaggaac ctcggcaccg ttccacggct aattctggcg catcgccggc
      601 gatgtccgct gcacccgctc ttccgcccaa aggcgggaag tccaccgatc ttgctatgcc
      661 gcagaagtgc caggagttga catcaagggc cgtgctaatt cgacgtccaa cagcagctca
      721 gcgctttcgc tgctcgctcg attaaattcc ctgttatccc gaaaaaattc cctgttatgg
      781 ctgcaaaaat tccctgttcc gttgcgtagg gaatttggct gcaagccttt gaattggctc
      841 gttgattaga tgcggaaatc gctgctgagg cccggaattt gcaaaattcc ctgttaattt
      901 ccctgttagc agggaatttg gtgcggagac tagttcgcag ttgactgcat cgtcagccag
      961 ccagtccggt ctctgtagct caggggcaga tacgagaaaa tgcgcgatca gggccgtcga
     1021 cagagcgcat cggggcttcc gagcaatgca tgataccgac tccggctgag tgattcctga
     1081 gattttcggg attcacggcg ggaatcgaaa cagttagctt gcggcgtcat gattcggtcg
     1141 gaggggcacg atcatgacga cgatcaaggc gcacttgagc agcgttgagc ttgaggcgtg
     1201 ttataagacg gcagtcgatc cgatcggcaa gagccatttt catgcgctgt ggctgttgtc
     1261 gtcaggctat gaggtcgagg aagtcgcgga gctattgtcg ttctctccgc ggtgggtgcg
     1321 tgcgctgctc aagcggtaca atgagggcgg tgccgaagca cttggcgatc agcgtgtcca
     1381 caacggaacg aagccgacga tcctcacgcc cgaggcgctg gcggctctca aggagcggat
     1441 caagatgccg cctgatgacg gcgggctttg gacgggtccc aagattgcgc gctggcttgc
     1501 caggttccac ggggtccgat cggtgcatga tcagcgcggg tgggacgcgc tgatcgcgat
     1561 cggatattcg atccagcagc cgcggccgcg tcaccctgat gcggcgaccg atgaggatcg
     1621 cgccgcgctc aaaaaaagct tgcggcggcg gctgacgagg agcagcgcca gcatccgggc
     1681 gcgagcgttg aaatctgggc gatggatgaa catcgcatcg gtctgaagcc gatcgtgcgg
     1741 ggggtgtggg cgccggtcgg ggagcggccg attgcattcg gccatcatcg tttcgagtgg
     1801 ctctatgtga caggattcgt cgaaccggcg acaggccgga ccgtgtggaa catcgccaac
     1861 gccgtctgca aggaactgtt cgaactcatc cttgctgatt ttgcaaaatc ggtcggcgcc
     1921 agcgccaaca agcgcatcgt gctgcagctc gacaatgcgg gttggcacgg gccagaaaac
     1981 atcgccgtac ccgatggcat ccgcctcgtg tttcagcccg cccacagccc ggagctacag
     2041 ccggctgaac atctttggac cttcgtcgac gaaccgctcg ccaactgctg ctttgagacc
     2101 atcggcagcc ttgaccgggc gatcgccaac cgctgcctcg cccttatcca gcagccagac
     2161 acgatccgcg acagcacgct gttccactgg tggccatgtc tgcgctccag gacgtaatca
     2221 aacggagtgg gtatgacgcc ggcgagattg agcgcgtgtc accgcatttg cgcgcaaccc
     2281 gaacgccggc atcagacagc tacgcggcta atatagggtc ggaagcaacg gttcagctgg
     2341 cgttcacaca gacgggtgct accgctgagc acctcggctc gcaacgcgac gaggacggct
     2401 actgcactgc cgcccgccga acgtgacggg agtacaggac cgtagccgtc cttgcacctc
     2461 agaacgaaag gaccaagatc gaccatctgg cgatgtctcg tcttcagtcg aggcccggga
     2521 caacattcag cacgtcgtac tcgacggctt cctccaggtg cttccagacg gcctgatcgg
     2581 cggcacccat tgcctgcagg atttcatcca cc
//
LOCUS       DATLJT010000314         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2085219,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000314 DATLJT010000000
VERSION     DATLJT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085219"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1820
                     /locus_tag="VM910_07105"
     CDS             27..1820
                     /locus_tag="VM910_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI62346.1"
                     /translation="MERRLAAIVCADVAGYSRMMGADEAGTHATFKAHRSAIHPIILN
                     HGGRVVKNTGDGFLLEFPSVVGAVEAAIAMQALMAERNNHLPADRAMHFRLGVHMGDV
                     MADEDELFGDDVNIAVRLESVAAPGGVAISEKAYSEAGAYLSEQLVDAGMYRFKNIRD
                     PIHVFTWEPTGSDAHDERRGETSVLPAQYRTAIVGVLPFVNLSGNADEYFADGLTEDL
                     IHALSLQSFYRVLGRSATFPFKGKALSARLIAREIDATYLVQGSVRRSGTKIRVTAEL
                     IAPENGEQLWTGRYDRDMGDLFALQDEITTSLSAAIAPEIFRAEASASARKTDLTAWD
                     RFLKGLSHYYRETEADFEASIDLFKEAIALDPTLAIARAYLATIMVQSMHYGWIKSTR
                     ELWGVAMGLAEDSVRLDPRSSFGFSILAYLHAMQGRHEAAQEAIRKAIELNPYDMGAR
                     GVQGVCHLVAGEHREAIELLSMAAQRGNGDPRYQWASSNAFSHYLLGQYDACLSWARE
                     ALYVNPNHLQVLAVRAAALARLGRSEEAAKAREVLLGNYPQLTVERHLRNFKWKNPAD
                     IDHYRQGLLKAGVPFGRLTLVESPPRRAADS"
     gene            complement(1849..>2481)
                     /locus_tag="VM910_07110"
     CDS             complement(1849..>2481)
                     /locus_tag="VM910_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62347.1"
                     /translation="FVRQVVLPDCPPPHFALAHSMGGTVMLRLAHAGKRWFDRMVLSA
                     PMIDLPGRLTSFPVRAFVKVLRLTGQGGRYVPGGGDRLTGTDSFINNPLTSDPVRYAR
                     NAAILEEDPTLGLASPTVAWADTAFGAMRAFRAYDYPSRIRQPILMLAASNDAVVSTS
                     AIEEFAYHLRAGSHLVIAGSKHEILQEQDRYRAQFWAAFDAFVPGTPLFG"
BASE COUNT          479 a          803 c          786 g          413 t
ORIGIN      
        1 cggggtcctg gagcaccaga taaggtatgg aaagacgcct tgccgccatc gtctgtgctg
       61 acgtcgccgg ttactcgcgc atgatgggtg ccgacgaggc cggcacccat gccacgttca
      121 aggcccatcg cagcgcgatc catccgatca ttctcaatca cggcggccgc gtcgtgaaaa
      181 ataccggcga cgggtttctg ctcgaattcc cgagcgtggt cggcgccgtc gaggctgcaa
      241 tcgccatgca ggccctgatg gcggaacgca acaaccatct gcccgccgat cgcgccatgc
      301 acttccggct cggcgtccac atgggcgatg tgatggccga cgaggacgaa ctgttcgggg
      361 acgatgtcaa tatcgcagtt cgcctcgaat cggtcgccgc ccccggaggc gtcgcgatct
      421 cggagaaggc ctacagcgaa gctggcgcgt atctcagcga gcagctcgtc gatgccggga
      481 tgtaccgttt caagaacatc agggacccga tccacgtctt cacctgggaa ccgaccgggt
      541 cggatgcaca cgacgagcgg cgcggcgaga cctcggtcct gccggcgcag tatcgaaccg
      601 caatcgtcgg ggttctgccc ttcgtcaacc tcagtggcaa cgccgacgaa tattttgccg
      661 acggcctcac cgaggacctg atccatgcgc tttcgctgca atccttctat cgcgtgctcg
      721 gcagaagcgc gacgttcccg ttcaagggca aagccctgag cgcgcgcctg atcgcgcgcg
      781 agatcgatgc gacctatctg gtccagggtt cggtccggcg ctcggggacg aagatacgcg
      841 tcaccgccga gttgatcgca ccggaaaacg gcgagcaact ctggaccggc cgatatgacc
      901 gggacatggg cgatctgttc gcgctgcagg acgaaatcac gactagcctg tccgcagcca
      961 ttgcgcccga gatttttcgc gcggaggcct ccgcatcggc gcgaaaaacc gacctcaccg
     1021 catgggatcg cttcctgaaa ggcctgtcgc attattatcg ggagaccgaa gcggatttcg
     1081 aggcgtcgat cgacctgttc aaggaggcga tcgcgctcga ccccacgctt gcaatcgcac
     1141 gagcctatct cgccaccatc atggtccaga gcatgcatta tggatggatc aagagcacgc
     1201 gcgaactgtg gggtgtcgcc atgggtctcg ccgaagacag cgtccggctt gatccgcgat
     1261 cctcattcgg gttctcgatt ctcgcctatc ttcacgcgat gcagggccgt cacgaggcgg
     1321 cgcaggaagc catcaggaag gccatcgagc tgaaccctta cgacatggga gcgcgcggcg
     1381 tgcagggagt ctgtcacctc gtcgccggcg aacatcgcga ggcgatcgaa ctcctgtcga
     1441 tggccgccca gcgcggcaac ggcgatcccc gctatcaatg ggcgtcatcg aacgcgttca
     1501 gtcattatct tctcgggcaa tacgatgcgt gcctgtcctg ggcgcgggaa gcgctttacg
     1561 tcaatcccaa ccatctccag gtgctggcgg tacgcgcagc cgcccttgcg cgactgggac
     1621 gcagcgaaga ggcggccaag gcccgcgaag tgttgctggg caactatccg cagctcaccg
     1681 tcgaacgcca tctgcgtaat ttcaagtgga agaacccggc cgacatcgat cactatcgac
     1741 aggggctttt gaaggcaggc gtccccttcg gccgactgac gctggtcgag tcccccccaa
     1801 ggcgagccgc ggattcctga gcgcgctcgc ggcgcacggc tcaacccctc aaccgaacag
     1861 cggtgtgccc ggcacgaaag cgtcgaatgc cgcccagaac tgcgcgcgat agcgatcctg
     1921 ctcctgcagg atctcgtgct tggagccggc gatgacgaga tgcgagccgg cgcgcaggtg
     1981 gtaggcgaac tcctcgatcg ccgatgtcga gaccacggcg tcgttggacg cggcgagcat
     2041 caggatcggc tgacggatcc gcgacggata atcgtaggcc ctgaaggcac gcatcgcgcc
     2101 gaaggcggta tcggcccagg ccaccgtcgg cgaagcgagg cctagcgtcg gatcctcctc
     2161 caggatcgcg gcgttgcgtg cgtagcgcac gggatcgctg gtcaagggat tgttgatgaa
     2221 ggaatcggtg ccggtgagcc ggtcgccgcc gccgggcacg tagcggccgc cctggccggt
     2281 cagccgcaac accttcacga acgcgcgcac cggaaacgag gtcagccggc ccggcaggtc
     2341 gatcatcggc gccgacagca ccatgcggtc gaaccagcgc ttgcccgcat gcgccagccg
     2401 aagcatcacc gtgccgccca tcgaatgggc aagtgcaaaa tgaggtggcg gacaatcggg
     2461 cagcaccacc tgccgcacga a
//
LOCUS       DATLJT010000315         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_247194,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000315 DATLJT010000000
VERSION     DATLJT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247194"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            501..680
                     /locus_tag="VM910_07115"
     CDS             501..680
                     /locus_tag="VM910_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62348.1"
                     /translation="MQLHLAFLELTDPPPSPTAPTIVPPWEQIDEAARMPALEVLARL
                     IASMLGAPEEEMPDE"
     gene            673..2892
                     /locus_tag="VM910_07120"
     CDS             673..2892
                     /locus_tag="VM910_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI62349.1"
                     /translation="MNERAKITPSHLSRQAIVYLRQSSAAQVENNRESTERQYALASK
                     ARELGWPEDRIIVIDEDLGLSGSGSVARSGFARLTAEVALARVGLVLGLEVSRLARNN
                     ADWHRLIDLAGITDTLIGDADGIYHPGLFNDRLLLGLKGTMSEAELHFLRARLNGGIR
                     NKAARGELRRGLPVGFVWGDEDGEVLFHPDEAVVTTIKSVFERFAETGSARRVWLWFR
                     TEGVKFPLQLHAHAEIRWVEASYHASHHVLTNPVYAGAYVYGKTRSETTLDASGARKK
                     RLRRLPREQWQIVIREHHKGYIDWSTYEANQARIATNTRPRPHNGSSSGGAVREGGAL
                     LQGLASCGHCGRRLRTHYSGRNSAPGYHCGGENLANGRGSYCLNIGGVQIDEAVTRAF
                     IAALEPIKLAATVAAAERLEADREATLKQWRLGVERANYEAIRAERRYRAVDPDNRLV
                     ARGLEREWEESLSALEAAKAELARREAERPRVLSEAERASLLAVGADLNAVWNAPTTS
                     PRDRKELLRTLLEEVIIKVERDKYAAHITMRWKGGALTELDLYLPHSKPQIVRTDEDT
                     IALVRRLAAHYPDAMIAGILSRQGRKTAYGHRFQVHHVAGLRRQWKIPRYERKAESSE
                     GELLTVKKAAAALGVATSTMHRLLNDGIIGGEQLTPGAPWRIRLTDDIKARFSQDAGE
                     GFLPMREAIRALGVSRQTVLQRVKRGELEAIHVIRGKQKGLRIKVVQQQPNLFDHSS"
     gene            complement(2880..>3521)
                     /locus_tag="VM910_07125"
     CDS             complement(2880..>3521)
                     /locus_tag="VM910_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HVI62350.1"
                     /translation="AAKPTNKAGVPAAEPAEQTAGTKRNTGEQSTHRTQGCVRVTQAL
                     DHVRQAPRLRKEEKFTALFHHLSIDLFGEAFFALKRDAAPGVDGVTWRAYEVDLDLKL
                     TDLASRVQRGAYRALPSRRTYIPKADGRQRPLAVSALEDKIVQRATAEVLNCIFEEDF
                     LGFSYGFRPGRGQHNALDALCVGIDSKKVNYILDGDIRSFLYAASYCPPTHEE"
BASE COUNT          687 a         1143 c         1035 g          656 t
ORIGIN      
        1 gccaagaggg gactgattac acctccttgc ggagttcctg cactgcgcgg cacgatgctt
       61 ccgtcctcca tctgcaccga cgccttcagc cagcgttcga tgtagagcag cacccaaggg
      121 cagtccgtat gttggcggac cttgagcatc agctcccaat caatactgtc gaaggaaagt
      181 acgacagact acccacctcc aggatctccc aaaatcagtg gtgattacgc gcaagcggca
      241 cccattcgag ggccaatcgc ttgcagtgat taccagcatc cgccggcgcg gcgtgcgtct
      301 cgttctcgct atattgccga acggcacccg ctcgctcatt ccggccgcct ggactgattg
      361 gaatgcggag ccgggctctg ccccaacttc ccacatttcc cacgatctcg gcaggctcgg
      421 tgatttgctc ctgctgcgca agatcatcaa cgctctcctc agccggccct ctgagtcggc
      481 gccgcatgag ggaagcagcc atgcaattgc acctcgcatt tctcgaactg acggatccgc
      541 cgccgagccc gacagcacca acaatcgttc cgccgtggga gcaaatcgac gaggctgcgc
      601 ggatgcccgc actcgaggtc ctcgctcggc tcatcgcgtc catgctcggc gcgccagagg
      661 aggagatgcc cgatgaatga gcgagccaag atcacgccga gccatctctc tcgccaggcg
      721 atcgtctatt tgcggcaatc cagtgccgcc caggtcgaga acaatcgcga gtcgacggag
      781 cggcaatatg cgctcgccag caaggcacgg gagctcggct ggcctgagga tcggatcatt
      841 gtcatcgacg aggatctcgg cctttccggc tccggttcgg tcgcacgatc cggctttgcc
      901 cgcctcaccg ccgaagtggc gctcgcacgc gtcggtcttg tgctcggcct cgaggtgtcg
      961 cggctcgctc gcaacaacgc cgattggcac cggctcattg atctcgcggg tatcaccgat
     1021 acgctaatcg gcgatgccga tggcatctat catcccgggc tcttcaacga ccgcctgttg
     1081 ctcggtctta aaggaacgat gagcgaggcc gagcttcact tcttacgcgc gcgcctcaac
     1141 ggcggcattc gcaataaggc agcacgcggc gagctcaggc gtggcctccc ggtcggcttc
     1201 gtctggggcg acgaggatgg cgaggtgctc tttcacccag acgaagccgt cgtcacaacc
     1261 atcaagagcg tgtttgagcg cttcgccgag accggctcag cacgccgcgt ctggctgtgg
     1321 ttccgtacgg aaggcgtgaa gttcccactg cagttgcatg ctcatgccga gatccgctgg
     1381 gtcgaggcca gctatcacgc cagccaccac gtgctcacca accccgtcta tgcaggcgcc
     1441 tatgtctatg gcaagacgcg aagcgagacg acgctcgatg cctcaggcgc acgtaagaaa
     1501 cgcctccggc gcctaccgcg cgagcagtgg caaatagtca ttcgcgaaca tcataagggt
     1561 tacatcgact ggtctaccta tgaagccaac caagcacgca tcgccacgaa cacgcgacca
     1621 aggccgcaca atggttcaag ttcgggtggc gccgtgagag agggtggcgc actcctgcaa
     1681 ggtctcgcca gctgcggcca ctgcggccgt cgtctgcgca cgcactatag cggtcgaaac
     1741 tcggctcccg gataccattg cggcggcgag aacctcgcca acgggcgcgg cagctactgt
     1801 ctcaacatcg gcggtgtcca gatcgacgag gccgtcacgc gggccttcat tgcggcactt
     1861 gagccaatca agcttgccgc caccgtcgct gctgccgagc gcctcgaggc cgatcgcgaa
     1921 gcgaccctca agcaatggcg cctcggtgtc gagcgggcga attacgaggc gatccgggcc
     1981 gagcggcgct atcgcgccgt cgatcccgac aatcgcctgg ttgcccgcgg cctcgagcgc
     2041 gaatgggaag agagcctgag cgcgctcgaa gccgcaaagg ccgagctcgc ccgtcgcgag
     2101 gcggaacgac cacgcgtgct ctctgaggcg gagcgcgcca gcttgctggc ggtcggagca
     2161 gatctcaacg ccgtgtggaa cgcaccaacg acttcaccgc gcgaccgcaa ggaattgctc
     2221 agaacactgc tcgaagaagt cattatcaaa gtcgagcgcg acaagtacgc ggcgcatatc
     2281 acaatgcgct ggaagggtgg cgcgcttact gaactcgacc tctatcttcc gcactccaag
     2341 ccacaaatcg ttcgcaccga cgaggatacg attgcgctcg tgcggcgctt ggctgcgcat
     2401 tatcccgatg cgatgatcgc cggcattctt agtcggcagg gtcgcaaaac cgcttacggt
     2461 catcggttcc aggttcacca tgtcgccggt ttgcgccgtc aatggaaaat cccgcgctat
     2521 gagcgaaaag cggaatcttc cgagggtgaa ctcttgacgg ttaagaaagc agctgctgcg
     2581 cttggcgttg cgacatcgac aatgcatcgc ctactcaacg acggcatcat cggcggcgag
     2641 cagctcacgc ccggtgcccc ctggcgcata cgactgaccg atgacattaa agcacgattc
     2701 agccaggatg ccggggaggg cttcctaccc atgcgcgaag ctatacgcgc acttggcgtc
     2761 tcacgccaaa ccgtgttgca gcgtgtaaag cgcggcgagc tcgaagcaat ccatgtgatc
     2821 cgcggcaagc aaaaaggtct gcgcatcaaa gtggtacagc aacagcctaa tctgttcgat
     2881 cactcctcat gagttggggg gcagtatgac gcggcataca aaaagctccg gatatcgcca
     2941 tctagaatgt agttcacctt cttgctgtcg atcccgacgc aaagcgcatc cagcgcattg
     3001 tgctggccac gccccggccg gaacccataa ctgaacccga ggaaatcctc ctcgaaaatg
     3061 cagttcagca cctcggcggt cgcccgctgg acgatcttgt cttcgagggc agacaccgca
     3121 agtgggcgct gtcttccgtc ggcctttggt atatacgttc ggcgcgacgg tagagcccgg
     3181 tatgctcccc gttggacccg cgaggccaga tcagtgagtt ttaggtcaag gtctacctcg
     3241 taggctcgcc atgtcacccc atccactccg ggggcggcat cgcgtttgag cgcgaagaac
     3301 gcttccccga acagatcgat gctgaggtgg tggaagagcg ccgtgaactt ctcctccttc
     3361 cttagccttg gggcttgccg gacgtggtcg agcgcctgtg tcacgcgtac acagccctgt
     3421 gtccggtgcg tgctttgctc gcccgtgttc ctcttggtcc ccgccgtttg ctccgccggc
     3481 tccgcagcgg gcacgcccgc cttgttggtc ggcttcgccg c
//
LOCUS       DATLJT010000316         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3234277,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000316 DATLJT010000000
VERSION     DATLJT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234277"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VM910_07130"
     CDS             complement(<1..1140)
                     /locus_tag="VM910_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI62351.1"
                     /translation="MKRFNLSAWAVSHPALVLFLVVALGVAGFFSYQRLGRAEDPFFT
                     VKVVNVSAIWPGATAQEMQTQVADPIEKKLQELPYFEKVQTYSKPSFTAMQVTFRDST
                     PPKDVPYLFYLLRKKLVDVQDQLPAGLLGPVVNDEFSDVDSMLYMMTGDGADYKQLKK
                     VAEGFRQRLLKVDGVTKVDLYGVQDERIFVEFSHAKLATLGITPQALFDSLAKQNNVV
                     PAGMVETSSQRVPLRITGALDGAKAVAETPVESNGRVFRLGDIATVTHGFVDPPSFKV
                     RQEGKPAIGIGVVTAKGANILDLGKEVTAATSEFMKAVPQGINVELIADQPEVVEHAV
                     GEFIHSFVEALAIVLFVSFLALGWRTGIVVAASVPLVLAIVFIVMN"
     gene            complement(1221..2327)
                     /locus_tag="VM910_07135"
     CDS             complement(1221..2327)
                     /locus_tag="VM910_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI62352.1"
                     /translation="MLARSILSNYYRLLAGVLLALVAIALAGCNDQVAQKPTPVRPVL
                     VATAHYEAETPERSFVGTIRPRIEADMGFRVPGKVAKRLVEVGQTVEVGQPLATLDEI
                     DLRLQAEQAEAEFNAATGVLAQAAAAEQRAKDLRAKGWTTDAQLDSAKATADEARARL
                     NRAERSIDLTRNSLSYATLVADARGVVTATLIEPGQVVAAGQTAIRVARFAEKEAVVA
                     IPETLVSRAKTGAATVTLWSDPDRKFAARLREISPSADPATRTYLAKFSLPGAGDEVE
                     LGMTATLTLCDPATMRVARLPLSALFDQGDKPSLYVVDDKGDVALKPVQVKSYESNDV
                     VITGGVEEGARVVTLGVQKIDPTVKVKVVSSLSF"
BASE COUNT          481 a          814 c          807 g          458 t
ORIGIN      
        1 attcatcacg atgaagacga tcgcaagcac cagcggcacc gaggccgcga ccacgatgcc
       61 ggtgcgccag ccgagcgcga ggaaggagac gaacagcacg atcgcgagcg cctcgacgaa
      121 ggaatggatg aattcgccga ccgcgtgctc gaccacttcc ggctggtcgg cgatcagctc
      181 gacattgatg ccctgcggca ccgccttcat gaattcggag gtcgcggcgg tgacctcctt
      241 gccgaggtcc agaatgttgg cgcccttggc ggtgacgaca ccgatgccga ttgcaggctt
      301 gccttcctga cgcaccttga agctcggcgg atcgacgaag ccgtgggtga cggttgcaat
      361 atcgccgagg cggaacacgc gcccgttgct ctcgaccggc gtctcggcga ccgccttggc
      421 gccgtcgagc gcaccggtga tgcgcagcgg cacgcgctgg gaagaggttt caaccatgcc
      481 ggccggcacg acattgttct gcttcgccag cgaatcgaac agcgcctgcg gcgtgatgcc
      541 gagcgtcgcg agcttggcgt gcgagaattc gacgaagatg cgttcatcct gaacgccgta
      601 gaggtcgacc ttggtgacgc catcgacctt cagcaaccgc tgccggaaac cttcggctac
      661 cttcttgagc tgcttatagt cggcgccgtc gccggtcatc atgtagagca tggagtcgac
      721 gtcggagaat tcgtcgttga cgaccgggcc gagcaggccg gcgggcaact ggtcctgcac
      781 gtcgaccagc ttcttgcgca gcagatagaa caggtagggc acgtccttcg gcggcgtcga
      841 atcgcggaag gtaacctgca tcgccgtgaa cgacggcttc gaataggtct gcaccttctc
      901 gaaatagggc agttcctgca gcttcttttc gatggggtcg gcgacctggg tctgcatttc
      961 ctgggcggtc gcgcccggcc agatcgcgga gacgttgaca accttcaccg tgaagaaggg
     1021 atcttcagcg cggccgagcc gctgatagga gaagaagccg gcgacgccga gcgccaccac
     1081 cagaaacagc accagtgcgg gatggctgac agcccaggcg gacagattga agcgcttcat
     1141 cgtaactctc caatgcgaat accgtctctc ttcttcattc cggggcgatg cgaaacatcg
     1201 atcccggaac gaccgacacc tcagaacgac agcgacgaca ccaccttgac cttcaccgtc
     1261 ggatcgatct tttgcacgcc gagcgtgacc accctggcgc cctcctccac gccgcccgtg
     1321 atgacgacgt cattactctc gtaggatttc acctgaaccg gcttcagcgc gacgtcgcct
     1381 ttatcgtcaa cgacatagag ggacggcttg tcgccctggt cgaacagggc cgacaacggc
     1441 agccgcgcca cgcgcatggt cgcgggatcg cacagcgtca gcgttgcggt catgccgagt
     1501 tcgacttcat cgccggctcc gggcagcgag aatttggcaa gataggtgcg agtcgcggga
     1561 tcggcgctcg gcgagatctc gcgcaggcgc gccgcaaatt tcctgtcagg gtccgaccac
     1621 agcgtcacgg tcgcggctcc cgtcttggcc cgggagacca gtgtctcggg gatcgcgacg
     1681 acggcttcct tctcggcaaa gcgcgcgacg cggatcgcgg tctgtcccgc agccacgacc
     1741 tggccgggct cgatcagggt cgcggtgacg acgccgcgcg cgtcagcgac gagcgtcgcg
     1801 taggacagcg agttcctggt gaggtcgatc gaacgctcgg cccgattgag ccgcgcgcgc
     1861 gcctcgtcgg cagtggcttt cgcggaatcg agttgcgcgt cggtggtcca tcccttggcg
     1921 cgcagatcct tggcgcgctg ctcggcggcg gcggcctgcg ccaacacgcc cgttgcggca
     1981 ttgaattcgg cttccgcctg ctcggcctgc agcctgagat cgatctcatc gagggtcgcg
     2041 agcggctgcc cgacctcgac ggtctgacca acctcgacca ggcgtttggc gaccttgccg
     2101 gggacgcgga agcccatgtc ggcctcgatc cgggggcgga tggtgccgac aaagctgcgc
     2161 tccggagtct ccgcctcgta atgggcggtt gcgacaagga caggccgcac gggggtcggc
     2221 ttttgcgcga cctggtcgtt gcaaccggcg agcgcgatcg ccacaagggc cagcaacaca
     2281 cccgccaaga gcctgtaata attcgataaa atcgaccgag ccaacatcgg atactcccgc
     2341 tcttggatct gataggaagt atcgagttat caatgatgaa agtcaatatt tgtcaataat
     2401 caggatttcg tgaacgttaa ttaatgtccg gccccggaca cacgcccggt cgcgcgccgt
     2461 ggcgaagact cactttgcca gcccgcaaac tccgcgttgc tctcgtggtc accgactaac
     2521 tggactcgcg gtcaatcgcc gagttgaaac cgctcgaacc
//
LOCUS       DATLJT010000317         5196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2085306,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000317 DATLJT010000000
VERSION     DATLJT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085306"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM910_07140"
     CDS             complement(<1..692)
                     /locus_tag="VM910_07140"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HVI62353.1"
                     /translation="MDVITPHDLSDALGELALDLRWTWSHEADALWRRVDEEAWRRTQ
                     NPWTILQDISAERLRALATDRSFTTELERLTRARQEYLRTPGWFASAHGTVALSGVAY
                     FSMEFGLGEALPLYAGGLGILAGDFLKTASDIGLPVIGISLLYQEGYFRQFIDASGAQ
                     QEAYPFNDPGSMPIQPAKGPDGAWLHIHLDLPGRPVQLRVWQALVGRVRLYLLDANDP
                     LNSPADRAITGKL"
     gene            1124..2479
                     /locus_tag="VM910_07145"
     CDS             1124..2479
                     /locus_tag="VM910_07145"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI62354.1"
                     /translation="MPQPILAVGVDPAKRLHRAVAVLFPDEVVLDVELPNAFDAVANL
                     DLRLCELANQHGADLVYGLEDHRRYGRLFCQVLTERGRDVRVVNPLWTNRQRAFYGQD
                     KDDAIDGRSIAAVVLRRHHQLPLASDFGEVAQAIREAERSLQDLSKVGTQLVNRLHLQ
                     LSDTYTAAYEEYFGKLKSPLALRFFRRFPLPQDLVGHNVEMLASLLLDLAGGKVGPHK
                     GGNRLIAMRSKAQAILETSGAMRASPRTLALELKAELIRQLCDELLANHDRIIRLERM
                     LREQLLPATKQTITTITGISTILAATIIGETGSISRFRSPAAFAVYNGTAPARNSTGG
                     RDRHKARRDCNHRLKRAFYLAARAAVLHDPIAKAYHQSCKSRGLSYVEALKRVARRMS
                     DVVYALLKSGKAYDRSIIEHAIERRREQAAHAARSHAKQSLVHSRPRKSNSPLSQRQV
                     M"
     gene            complement(2931..4047)
                     /locus_tag="VM910_07150"
                     /pseudo
     CDS             complement(2931..4047)
                     /locus_tag="VM910_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596868.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4060..>5196)
                     /locus_tag="VM910_07155"
     CDS             complement(4060..>5196)
                     /locus_tag="VM910_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62355.1"
                     /translation="AAEPVFSVQRLFACTIRESLELLRDPIRLGFALFGTTFLMLVFG
                     FGISTDVNNLSLAVLDRDQSHESRAYLEEMRGSPYFLEKPKLTDYADLERRLENGNIS
                     AGIEIPPGFGRDIARGRPVWVAAWVDGARPFVAQTIRGYLQGMHQLYLADPAVKTSLG
                     TSPPPADIEIRFKYNQDFDSIYAMVPSTMALELALFPAILMALAIVREKELGSITNLY
                     VTPVTRVEFLVGKQLPYVAVAMVNFALMFVMALLVFKVPLKGSFPTLLLGTLIYVSAT
                     TAYGMLISAFARTQIAALFGTAILTVLPATMFAGMMVPVSSLSGMAQIMGRLFPMTYF
                     LPVSVGTFTKGLGFSDLAPKLAALAVFIPALTMLSLLLLRNQER"
BASE COUNT         1134 a         1543 c         1584 g          935 t
ORIGIN      
        1 agcttgccgg tgatggcccg gtccgccggg ctgttgagcg gatcattggc gtcgagaagg
       61 tagagtctga cccgcccgac cagcgcctgc cagacccgca gctggacggg gcgtccgggc
      121 aggtccaggt gaatgtggag ccaagccccg tccggccctt ttgctggctg gatcggcatc
      181 gagcccgggt cgttgaacgg gtaagcctcc tgctgcgcac cggaggcatc gatgaactgc
      241 cggaaataac cctcttgata caacaaactg atgccgatga ccggcaggcc gatatcgctg
      301 gccgttttca gaaaatcacc cgccagaatg ccaagcccgc cggcatagag cggcaacgcc
      361 tcaccgaggc cgaactccat gctgaaatag gcgacccccg acagggcgac agtgccgtgg
      421 gccgatgcga accagccggg tgtcctgaga tactcttgcc gcgcccgggt gagccgttcg
      481 agctcggtgg tgaacgagcg atcggtggca agcgcccgca gccgttcggc tgaaatgtct
      541 tgcaggatgg tccacgggtt ttgcgtgcgg cgccaagcct cctcatctac ccgccgccac
      601 aaggcatccg cctcgtggct ccaggtccag cgcaggtcga gcgcgagctc gccgagcgca
      661 tccgacagat catgcggggt gatgacatcc atcggtcagt gcgatgcggt tgcaagacat
      721 ggcacaatta cttccgtaca gcaccagccg cgttgaccca gatcaacacg ctcatcgtcc
      781 tgccaggcag ccttcgtcag gtcgggtata atcaagtcat catcaagtta tccactgttg
      841 ttgcggataa ctgtgtgtaa cgcatgtgaa tatcggggaa ggaatcctaa aatattttaa
      901 gaatgtccaa gtaaacctat cgtcttgtgc tgttcggatg ccgtcctcga tccgagacgg
      961 aagcggaaag gctggtcagg tcgtgccgga gttccaactc aaaggcttct tctatgctgg
     1021 ccccgcttcc ccccacggcc cgcgtccggt gacaagagag cgatgcgtca aacgctgtat
     1081 acctgatgac gccggagcgg cccctgctta ggaaggaacg tccatgccgc aaccgattct
     1141 tgccgtcggc gtcgaccccg caaaacgctt gcatcgggcc gtcgccgtgt tgttcccaga
     1201 tgaagtcgtt ctcgatgttg agttgcccaa tgcattcgac gccgtcgcca acctcgacct
     1261 tcgcctttgc gaactcgcga atcagcacgg cgccgacctg gtctacggcc tggaagacca
     1321 ccgccgctat ggccgcttat tctgccaagt attaacagaa cgcggccgcg acgtgcgcgt
     1381 tgtcaacccg ttgtggacaa accgccaacg ggcgttctac ggccaggata aagatgatgc
     1441 gattgacgga cgcagcatcg ctgctgtcgt cttgcgtcgt catcaccagc tgcctctggc
     1501 cagcgacttc ggagaggttg cgcaggcaat tcgtgaagcc gagcggagcc tgcaagacct
     1561 cagcaaggta ggtactcagt tggtcaaccg actgcatctt cagctcagtg acacctatac
     1621 agcagcctac gaggagtatt tcggtaagct aaaaagcccg ttggcgttgc gcttcttccg
     1681 ccgctttccg cttccgcaag acctggttgg ccacaatgta gagatgttgg caagcctgct
     1741 gctcgatctg gccggcggca aagtggggcc acataaaggc ggcaacaggc tgattgccat
     1801 gcgaagcaag gcgcaagcta tcctcgagac ctcgggcgcc atgcgtgcgt caccccgcac
     1861 acttgctttg gagctcaagg ccgaactgat ccgccagctc tgcgacgaat tgctggccaa
     1921 ccatgaccgc atcatccggc tcgagcgcat gctacgtgag cagttgctgc cggcgaccaa
     1981 acagaccatc acaaccatca ctgggatcag cactattctt gcagcgacga tcattgggga
     2041 gactggtagc atctcacggt tccgctcacc tgcggcgttc gccgtgtaca atggcaccgc
     2101 accggctcgc aacagcaccg gcggtcgcga tcgccacaaa gcacggcgcg attgcaatca
     2161 tcgcctcaag cgcgctttct atctggccgc acgggcagcg gttctgcatg atccgattgc
     2221 gaaagcctat catcaaagct gcaagagccg aggcctcagt tatgtcgagg cgctcaagcg
     2281 ggtcgcacga cggatgtctg acgtggtcta tgccttgctc aaatcaggca aggcctacga
     2341 tcgttcgatc atcgagcatg ccatcgaacg gcgccgggag caggcggcac acgcggcacg
     2401 cagccacgcg aaacaaagcc ttgtccactc ccgaccaagg aaatctaact cgccgctctc
     2461 gcagcggcaa gtgatgtgaa caaacttggg agaacctgta gacaacgctt gacttggtga
     2521 gagagcgcat agaggaggat cagatgcagg gatcacacga tcaatttatt ctgcaacgcg
     2581 gcgtcgagac ccgggatcct tgatcatgat cgagggtgat gacgggagct tcgagctgct
     2641 cgcgcatgct tggttcgcag gcaaaggatg gcgccgatga atgcccattc gacctggtcc
     2701 gaagtcgcaa taccgatggt gtcacttgtt ggtccacgca accgatcgcg atccccagca
     2761 cgtataccgc tggcatcgga tccgaggcga ttcgccccgc gcccaggatc gcatcgtcag
     2821 attgcaaggc gcagccgcac ccgaagtgac gcagtagcca cacctagatc tacacatgag
     2881 aagcagcatt acggcctgag attgtggcgg cggcagcgcg ggtcccgccg ctaggcaacc
     2941 gcaccggcga cccgccgaaa gcgcagcaag gcgagcccca gaaagacccc gccgatcagt
     3001 gcaacgatca cgaattgcgg ccaaaccact gtgaagccgg cgccccgata aaggatcgct
     3061 tgtgcaagcg acacgaaatg tgtcgagggc gatacctgca taatggtcgc cagccagggc
     3121 ggcatgcttt cgagcggcgt attactgccg gagagcatgt tcatcggcag aaagaccagc
     3181 aggtagagca gaccgagctg cggcatcgac cgcgcgatcg tcccaaggaa gatgccgatc
     3241 gcggtggcaa aaaacaggta gaccgcggtg ccgatcagga acagcgggat tgaccctgcg
     3301 atcggaacgc cgagcagtcc gtgcacgacg aaacgtatcg aaaggccgac tgccacgacg
     3361 atgacgaggc cattcgccca gatctttgac atcgcgatct cgaacggggt cagcggcatg
     3421 accaggagat ggtccattgt cccgtgctcc cgttcccgga caacggccgc ccccgccagg
     3481 atgatcgcga gcatcgtgat gctgttgatg atgcccatca cactggtgaa ccacgcggtt
     3541 gtgacgttcg ggttgaaggc gatgcgtacg agcaggttga tgtgcgacag cggattgcct
     3601 tcccgccgcg agagaaaatc gttgatctcg gtcatgatga tctgctgcgc atagtcggcc
     3661 ccgaggcctg cctggaccat cgcggttgca tcgacatcca gctggattgc ggggcggcgc
     3721 ccgccgagaa catcgcgctg aaaattcggc gggacgtcga tcacgaatgt ataccgtccg
     3781 ctattcatca atgggacgat gtcgcgctcg gcaatcggct gcggcgtctg gaaataagga
     3841 ggcaagaagg cgtgcgcgat gcgacgcgac agttccgaat ggtcctcgtc gacaatggcg
     3901 atcgacgcat tgtgcacttc ctgagaactg ctttgcgcct gcgcgataat ggcgagcgag
     3961 aaggcataga tcaccagccc cagcagcacg aagtcgcgct gaaagcttcg caacgccgag
     4021 ccaaaagatg tttgcgagtg cacgcatcga gtgggtggcc tagcgttcct gattacgcag
     4081 tagcagcagg ctcagcatgg ttagcgcggg gatgaagacc gcgagcgcgg ccagctttgg
     4141 agcgaggtcg gagaacccga ggcctttcgt aaaggtgccg acactgaccg gcaggaaata
     4201 ggtcatcggg aacagtcggc ccatgatctg cgccatcccc gagagcgagg agaccgggac
     4261 catcatgccg gcaaacatcg ttgccggcag aacggtcaga atggctgtac cgaagagggc
     4321 cgcaatctgg gtgcgggcaa aggccgagat cagcatgccg taggccgtgg tcgcgctgac
     4381 gtagatcagt gtcccaagca gcagcgtcgg aaagcttccc ttgagcggca ctttgaagac
     4441 gagcagcgcc atcacgaaca tcaatgcgaa gttgaccatc gcgaccgcga cgtaaggcag
     4501 ctgcttgccc acgaggaact cgacgcgcgt gaccggggtc acgtagaggt tggtgatcga
     4561 gccaagttct ttttcgcgga cgatggcgag cgccatcagg attgccggga acagcgccag
     4621 ctccagcgcc atggtcgacg gcaccatggc atagatgctg tcgaaatcct gattgtactt
     4681 gaacctgatc tcgatatcgg caggcggcgg cgatgttccc agtgacgtct tcaccgccgg
     4741 atcggcgaga tacaattgat gcataccctg caggtagccg cggatcgtct gggctacgaa
     4801 gggcctcgcg ccatccaccc aggcagcgac ccagacgggt cttccgcgcg cgatatcgcg
     4861 gccaaagccg ggtgggattt cgatgccggc actgatgttc ccgttctcga gccgccgctc
     4921 gagatcggcg taatcagtga gcttgggttt ttcgagaaag tagggagaac cgcgcatctc
     4981 ttcgagatag gcccggctct catggctctg gtcccggtcg agcacggcga gcgaaagatt
     5041 gttgacgtcg gtcgagatgc cgaagccaaa aacgagcatc aggaaggtcg taccgaagag
     5101 cgcgaagccg agccggatcg gatcgcgcaa gagttccagc gattcgcgga tcgtgcaggc
     5161 gaaaagtcgc tgaacactga acacgggctc cgctgc
//
LOCUS       DATLJT010000318         1669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1166697,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000318 DATLJT010000000
VERSION     DATLJT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166697"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VM910_07160"
     CDS             <1..725
                     /locus_tag="VM910_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HVI62356.1"
                     /translation="DNKPDVIFLVFNDHATAFSLDLIPTFAIGTAVEYMPADEGWGPR
                     PVPKVIGHPELAAHIAQSVIQQDFDLTIVNKMDVDHGLTVPLSLMCGQPDAWPCPVIP
                     FAVNVVQYPIPSGARCLALGQAIRRAVESYDEQLEVQVWGTGGMSHQLQGPRAGLINA
                     GWDKAFMDRLIGDPNGLSQMPHIQYVREAGSEGIELVMWLIARGAMGDVAGGPPPTVV
                     HRFYHVPASNTAVGHLILENPR"
     gene            743..>1669
                     /locus_tag="VM910_07165"
     CDS             743..>1669
                     /locus_tag="VM910_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVI62357.1"
                     /translation="MAKSIKVALAGAGAFGLRHLDGIRLIDGVEVISVVGRELEKTQG
                     TARKYGVGHATTDLADTLELPGLDAVILATPTQMHAAQALQCLEAGKHVQVEIPLADS
                     LRDAEAVVAMQKRTGLVAMCGHTRRFNPSHQFVRQRILASEFHIQQMDVQTYFFRRTN
                     MNALGQPRSWTDHLLWHHAAHTVDLFAYQCGSPIVRANALQGPIHPTLGIAMDMSIQL
                     KAENGAICTLSLSFNNDGPLGTFFRYIGDTATYIARYDDLFTGNDEKIDVSKLAVSMN
                     GIELQDREFFAAIREGREPNASVEQVLPCYRTL"
BASE COUNT          320 a          553 c          513 g          283 t
ORIGIN      
        1 aggacaacaa gccggacgtc atcttcctgg ttttcaacga ccatgccacc gccttcagcc
       61 ttgatctgat cccgaccttc gccatcggca cggcggtgga atacatgccg gccgacgaag
      121 gatgggggcc gcggccggtg ccgaaggtga tcggccatcc cgagcttgcc gcccatatcg
      181 cccagtcggt catccagcag gatttcgacc tcaccatcgt caataagatg gatgtcgacc
      241 acggtctgac ggttccgctg agcctcatgt gtggccagcc cgacgcctgg ccgtgcccgg
      301 tgatcccgtt tgccgtcaac gtcgtgcagt accccattcc ctcaggggcg cgctgcttgg
      361 cactcggcca ggcgatccgc cgcgccgtcg agagctacga cgagcagctc gaggttcagg
      421 tctggggcac cgggggcatg agccatcaat tacaggggcc gcgcgccggc ctgatcaatg
      481 ccggatggga taaggcgttc atggaccggt tgatcggcga tccaaacgga ctctcgcaaa
      541 tgcctcatat ccaatacgtg cgcgaggcgg gcagcgaagg aatcgagctt gtgatgtggc
      601 tcatcgctcg cggcgcgatg ggcgacgtcg caggcggacc tccgccgacc gtcgtgcacc
      661 ggttctatca cgtgccggcg tcgaacaccg ccgtcggcca tctaattctg gaaaacccac
      721 ggtagaggtt tggagagcgc ggatggcgaa atcgatcaag gtggcgctgg ccggcgcggg
      781 cgccttcggc ctcaggcatc tcgacggcat ccggctgatc gacggggtcg aggtcatttc
      841 cgtcgtcggg cgcgagctcg agaagacgca ggggacggcc aggaagtacg gtgtcggcca
      901 tgcgacgacc gatctcgccg acaccctgga gctcccgggc ctcgacgcgg tgatcctggc
      961 gacgccgacc cagatgcacg ccgcccaagc cctgcaatgc ctcgaggccg gtaagcacgt
     1021 ccaagtcgaa atccccctcg ccgacagcct gagggacgcg gaagccgtcg tcgcgatgca
     1081 aaagcgaacc ggcctcgtcg ccatgtgcgg ccacacccga cgctttaacc cgagccacca
     1141 gttcgtccgc cagcgaattc tggcgagcga gttccacatc cagcagatgg acgtgcagac
     1201 ctatttcttc cgtcgcacca acatgaacgc gctcggccag ccgcgatcct ggaccgacca
     1261 cctgctatgg caccacgcag cgcacacggt cgacctcttc gcctaccaat gcggctcgcc
     1321 gatcgtgagg gcgaatgcgc tgcaggggcc gatccatccg acgctcggca tcgccatgga
     1381 catgagcatt caattgaagg cggagaacgg cgcgatctgc acattgtcgc tgtcgttcaa
     1441 caacgatggg ccgctcggca ccttcttccg ctatatcggc gatactgcga cctacatcgc
     1501 gcgctacgat gaccttttta ccggcaacga cgagaagatc gacgtcagca agctcgccgt
     1561 ctcgatgaac ggcatcgaat tgcaggaccg cgaattcttc gccgccatcc gcgaaggccg
     1621 cgagccgaac gccagcgtag agcaggtgct gccgtgctat cggacgctg
//
LOCUS       DATLJT010000319         1931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3693641,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000319 DATLJT010000000
VERSION     DATLJT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693641"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VM910_07170"
     CDS             complement(<1..1149)
                     /locus_tag="VM910_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI62358.1"
                     /translation="MERQAATDDVLRLIASSPGDLHPVFDAILSNATRLCGAKFGVLF
                     RSERDALRAVALHGALLAYAEERRLNPVIRPNPDTLLGRALAAKRTLQIADIRDERAS
                     NQSASGTTSAQIVQLAGARTMVAVPMLKDNELVGAIVVYRQEVRPFTDGQIALLTNFA
                     NQAVIAIENTRLLNELRQRTSDLAESLEQQTATSEVLSVISSSPGQLEPVFHAMLENA
                     TRICEAKFGVLFRFTGELYEFAAEVGASPEFSEFLRRRGPFLPVPGTHLYRVTQTKQI
                     SHTADYAADAPEAPPVRLAGARSTVDVPMFKDGALIGVVSIYRQEVRPFTDKQISLLE
                     NFADQAVIAIENTRLLNELRQRTDDLSEALEQQTATSEVLGVISRSPGE"
     gene            complement(1470..>1931)
                     /locus_tag="VM910_07175"
     CDS             complement(1470..>1931)
                     /locus_tag="VM910_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62359.1"
                     /translation="DPNSRESMIESSEVQEAAQAIGQRIIVSKAGSELDLVPAFADTP
                     AALLIAGSPFFTRRRDQLVALAAQHAVPTVYEWREFATAGGLISYGPSLTDAYRQVGV
                     YAARILNGDKPADLPVQQPTKFELAINLKTAKALGLEVPPTLLTRADEVIE"
BASE COUNT          380 a          577 c          593 g          381 t
ORIGIN      
        1 ctcgccgggc gaacgtgaga tgacgcccaa tacctctgac gttgcggtct gctgctcaag
       61 ggcttcactt agatcatcgg tgcgttgccg cagctcattg agcaggcgcg tgttctcaat
      121 tgcgatgaca gcctgatcgg cgaagttctc caacaaggaa atctgcttgt cggtgaaggg
      181 gcggacctct tgtcggtaga tcgagacgac cccaatcagg gcaccatcct tgaacatcgg
      241 cacgtcaaca gtagatcgcg cgccggctag cctgacgggc ggggcttcgg gggcgtctgc
      301 agcatagtcc gcggtgtgac taatctgttt ggtctgtgtg acgcgataga ggtgggtgcc
      361 cggcacaggt aaaaacgggc cgcgccgcct caagaattcg gagaattccg gcgacgcgcc
      421 gacctccgcc gcaaattcgt acagctcgcc ggtgaagcgg aataggacac cgaacttggc
      481 ctcacagatg cgcgtcgcat tctccagcat ggcgtggaat accggctcca gctggccggg
      541 cgaggagctg atgacgctca acacctctga tgtggcggtc tgctgttcta gtgactcggc
      601 gagatcgctc gtgcgctggc gcagctcgtt cagtagacgc gtgttctcga tagcaatgac
      661 ggcctgattg gcaaaattcg tcaacagcgc gatctgccca tcggtgaatg ggcggacctc
      721 ttgccgatac acgacaatcg cgccaaccag ttcattgtcc ttgagcatgg gtacggctac
      781 catcgtgcgg gcgcccgcaa gctgcactat ttgcgcactg gtcgtgcctg acgccgattg
      841 attagaggcc cgctcatccc ggatgtcggc gatctggagt gtccgtttcg ccgcaagggc
      901 acgtccgagc aaggtatcgg ggttggggcg gattaccgga ttgagccgcc gctcctcggc
      961 gtaagccagc agtgcgccgt gcagggcgac ggcgcgaagc gcatcacgct cggaccgaaa
     1021 gagaacgcca aacttggccc cgcacagccg ggtcgcattc gataggatgg catcgaatac
     1081 cggatgcaaa tctcccggag aactcgcgat gaggcgcaac acatcgtcgg tcgccgcctg
     1141 ccgctccaac gcctcggcaa gctcgcgccg tgctattgct agtccctcta cgaggtcggc
     1201 atcggacacc tgcggcatcg cagccccatt gcgttgacaa ccgcgggatc agtcggtgac
     1261 agcatagcgg attggccagc atcaagtcag gggtttgctg cgctgcggga ttacggcccg
     1321 cctcatgtcg gtggggtcaa tcgctcccga tcgacatgac cgcgacgcgc gcccaatgtc
     1381 cgcttcacct ccagtagcgg tcaaacattg gcaccgcagc gaaacgccgc ttcgtgccag
     1441 gagccaacat cattgcagca aagaggccat tattcgatca cctcgtcggc gcgtgtgagc
     1501 agcgttggtg gcacttcgag gccgagtgcc ttggcggtct tgaggttgat agccagttcg
     1561 aacttcgttg gctgctgaac gggaagatca gccggcttgt ctccattgag aatacgggca
     1621 gcgtaaacgc caacttggcg atatgcgtcc gtaaggctgg ggccgtagct tataaggccg
     1681 cccgctgttg cgaactcacg ccactcgtaa actgtgggta ccgcgtgctg cgccgccaac
     1741 gccacaagtt gatcgcgccg ccgtgtaaaa aacggcgaac cggcaatcaa gagcgctgcc
     1801 ggtgtatcgg cgaaggcggg aaccaggtcc agttcgctac cggccttcga gacaataatt
     1861 cgctgcccga tggcttgagc cgcctcttgc acttcacttg actcgatcat ggattcccta
     1921 ctgtttggat c
//
LOCUS       DATLJT010000320        15025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1855421,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000320 DATLJT010000000
VERSION     DATLJT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15025
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855421"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VM910_07180"
     CDS             <1..471
                     /locus_tag="VM910_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HVI62360.1"
                     /translation="AGLIPIPLVDMAAVGGVQLQMLRRLSEIYGVPFSENRGKSIIAS
                     LAGAVIPASTATTTAVGVGSLVKSLPGIGTAIGALTMPVFSAGATYVIGKVFIQHFAS
                     GGTLLDFNPPDYREFIKAQKEKFAARSAAAPSSGPSASAARSASSKGGAATGGP"
     gene            829..2472
                     /locus_tag="VM910_07185"
     CDS             829..2472
                     /locus_tag="VM910_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HVI62361.1"
                     /translation="MSFFTLVRREMYGSLNRLIVISALGGISTAAILTAINSGAQAAD
                     SGKVSLWSAALFIVALFLFIKTQHYILITATAEIEAIIHKLRLRLMDHVRHSELLPLE
                     AIGRGEIVGAITKETATLTQMSNTLAFAAQGAVLIFFVAMYVAYLSLLAFALSVIILG
                     IAAVLFHAKSRQLRMASHEASNLEHRLFDRLMDLLNGFKEVRLNSVRSDTLFNDVVEM
                     SRVAANIKIRAQSETFRRMVFLQSALYGLLGAIAFAVPTFSGAMGAETVTKTITALVF
                     VVGTCFGLVQAIPILSAANTAADNIEQLEARLLATIPMTEVTAVEPSKRFHKIEMRNV
                     VFRYVDKWSDAVFQVGPIDFTLLSGDLVFITGGNGSGKSTFLKLLAGLYKPDSGEITF
                     DGVRVNDSTRQAYRELIAAIFPDYHLFEKLYGVPDPDPAEVDSLLAQMRLRDKTHLTD
                     GHFSTLDLSQGQRKRLALIVSLLEKRPLLLLDEWAADQDPEFRRKFYYDLLPELKRSG
                     ETVVAITHDDRYIDEMDLPARRLRMEDGRFAAQQTVENR"
     gene            2472..3737
                     /locus_tag="VM910_07190"
     CDS             2472..3737
                     /locus_tag="VM910_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVI62362.1"
                     /translation="MKAYLWSDGEPPPTTRRARWRRFAERHLPSVVIYLMVATLVAVV
                     LYPHMVVTVPSGYVGVLWKRFGGGTVLDPRRLKNEGFNLILPWNQVFLYDLRLQSFTE
                     SYNAISSDGVSLTATIIVRFRLQRDAVPVLHQAIGPNYEQVLVQPGIGSLTREVIAQY
                     TAEQVYSTARQEIQDKIRSLVEDRLSEKMMEHEGEESYRVSMRDTIILYDILVTGIEL
                     PAAIVAAINRKTEQYYVAEEYKFRVEREKRESERKKIEAEGIRDFQQTVSQGISESYL
                     RWRGIEATLQLSQSTNAKVVVIGSGKDGLPIILGNVDTPAPPAGSTSPSGTDTMNKES
                     TTAAGPAVPLEKTPAAGLATPTEKVAPAISGNPSAAPEASRSFWPLTLSDIETYLYRI
                     LRPEPNPEPKAAEPRSSQPSERPVAAQPR"
     gene            complement(3813..4709)
                     /locus_tag="VM910_07195"
     CDS             complement(3813..4709)
                     /locus_tag="VM910_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI62363.1"
                     /translation="MSQIRLAAMAVALSAAGFATTGLAQQAQRPQMETTKVDGTDNVY
                     IFRNGNHQSMFIVTKDGVIATDPIAYGRPTGGQDYIAEIKKVTDKPIKFLIYSHHHYD
                     HIAGGKPFKDAGATIIAHRRAKERLAVLKDPNTPLPDQVVSGAGRTIKLGGTTLELKY
                     FGLNHSDSNLVMWLPKEKIVFVVDTIPVGSFPGRGFIDIYPLETEEFIKKVLALDWER
                     LIPGHPGQPGGRLGTKKDAQDILTLMQEASAEMKKMGQDGKCWEPAEKDFKLPKYANW
                     PGYENGLPLVARRYCGLWGRGT"
     gene            complement(4827..5720)
                     /locus_tag="VM910_07200"
     CDS             complement(4827..5720)
                     /locus_tag="VM910_07200"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HVI62364.1"
                     /translation="MIYRTRRVVTTRLTTSKIICRYRGRAGAQAAVEAATSASVGHRV
                     EAGVTASPCERSIAGRIVDPRAACWVARTNTIHQGRELPPRMIVTVDPSLQSAIDALS
                     FPGIMIGHRLISPGDEDALLPEEAPAFALSVVKVRRASGAARIVARQLLKRLGQPECA
                     LPRTSGGAPAWPAGVVGSLAHDSRVAVAAVGMGRDVGAVGIDVEPAESLPSELLDLVA
                     TPQERLRLGDDPYHGRLLFVAKEAVYKAVYPLDQTFLDHHDVQVSFAERKAIVRNGRV
                     VELRFCIAAHLVALAFLPNLR"
     gene            5781..8765
                     /locus_tag="VM910_07205"
     CDS             5781..8765
                     /locus_tag="VM910_07205"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62365.1"
                     /translation="MLEAIGTVSQRWVGSPVARKEDQALLTGQARFIDDLSPIPGIRF
                     AAILRSPHPHARIGRIDFARARDLPGVRGIVTGKDVADLIGPVPSVVKAPLAYYPVAI
                     DRTRYVGEPVAVVVADTRYIAEDACDLIEVEYDVLPAVSDLRSATARDAPVIHEMAGS
                     NVISRRNFIYGNPDAAFAAAERVFEFSYSYPRYASTPMETFGVIAHFERAPDRYTVWS
                     NFQGPFVVQPLMAGALRVPGHRLRLITPPSSGGSFGIKQAVLSYIVLLAAVSRKTGVP
                     VKWIEDRAEHLTASSASSDRLGTVAAAFTKDGELTGLRFKNTANMGAHVRPPEPASLY
                     RMHAASNGCYKVPNISIDNELVVTNCTPVGLNRGYGGPQFYFALERIMDIAARGLGMD
                     PAELRRRNFIPADVFPYRAPAGAVFDAGDYDAALSELLRIADYDELKRRRDEVRRTGG
                     LFGIGLAAGVEPSGSNMAYVSLAQTAQDRRRADRKSGANASAVVTVDPSGQVTVRLCS
                     TPNGQGHATVAAQIVADALGLHPNDIDVVTEIDTLTSPWSIASGNYSNRFAAIVVDAV
                     AKCAKQVAQKIKLLAANALEAAPEEIELREGYARVRGQSNKGLPFRKAAARAHWDPAG
                     LPDNATPGIHEAAIVSPPVLGSPDDEDRIASAVTFGFVIDLAAVEIDRKTGEIRIDKY
                     VSVHDVGTQLNPKIVEGQVHGGFAHGLGAALFEELAYDERGNFLSGTFADYLCPTAVE
                     VPRVEIGHVETASPVNALGAKGMGDGSSMLTPAAITNAVADALGRDDISLPLNLRRVW
                     AFANGHEVTPRPMAAQARAERTAVGGALTGSGEVSLSAPVAEVWRRLLDPSELAAIIP
                     GCRGLTQDDPDRYSAQVVIGVAGIRGTYHVQIEMRDKKEKSSVRLVGKAFGALGFGSG
                     SGFVSLQPEAGGRTRLQYRYEADVGGKVAAVGQRMLGSVTRYLIAQFFHSLERRLRPA
                     GGEGWRTWWSRLRRHGRNGGGP"
     gene            8762..9640
                     /locus_tag="VM910_07210"
     CDS             8762..9640
                     /locus_tag="VM910_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI62366.1"
                     /translation="MKPVAFDYYRPEAVSEALALVSEMGDEAAVLAGGMTLGPMLNLR
                     MVRPRAVIDISRIQALKAISARSDVVVTGAGVVQGDALRSDVIARAAPLLVMALPWVG
                     HFQTRNRGTLGGSVAHADPSAEIPLALVVCGGTVVLQSRRRQRRVPAREFFLGTLTTQ
                     RRPDEIVTALEWPRAAPDAGHAFTEIAQRHGDFAIAAAGCSARLDGADRLEELSLGVG
                     GVESRPVAIDVKSFLGRPAGEIAASLAEHAAVSVTPMEDHSASADYRIALTKVLVERA
                     VNAALEMARQRRGRLQ"
     gene            9637..10176
                     /locus_tag="VM910_07215"
     CDS             9637..10176
                     /locus_tag="VM910_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI62367.1"
                     /translation="MTTLYRAAGNRFTVELTLNGQRRSGEAEPRMLLCDFLRHELQAF
                     GVHVGCEHGVCGACTVLVDGRAVRSCTQYAAQADGADIVTVEGLAEPGTLNEVQRAFA
                     KHHALQCGFCTPGILCSVTHFLRDTPGPNEREVREMLSGHICRCTGYASIVAAVLEAA
                     AVIGKGSRPWISAAHLPPR"
     gene            10143..11708
                     /locus_tag="VM910_07220"
     CDS             10143..11708
                     /locus_tag="VM910_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI62368.1"
                     /translation="MDLGSAFAAAVMRQPRQEALVEGNRRMTYVDWYRDIASVAGGLQ
                     DTGLRAGDHLVVVMRNRYEMATLYWACQLLGIVFTPVSWRATADEIGYCLEDAEAAAV
                     AFDGASGSAAAEAAATLKIDPRRVIVAADGKGEGMPFGRLRDSAAVAGPAAVGESSIC
                     LMLYTSGTTGRPKGVPRSHHSELMAAIAHIAQNQYRRGDSSLGVMPFFHTMGVRILLS
                     TALLNGRLVCVPEYGPEEVLRLISEERLTTLFLVPTMFHDVLRHPKFDSYDLRSLSRV
                     GFAGMTMTPALIERCLDQLKPDLFVNYYGSSEIYTFSYCDHLGRKPGCAGRAGMNQII
                     RVVSPARDDDIEIDLQPGVAGEIVASMRSPEAFSGYWKRPDADAKAIQGRWYRTGDLG
                     KLDEDGELYVVGRVDDMIISGGENIFPEEVEDTLARSKLVAGCAVVGMPDERLGAKVV
                     AFVEPAVAQLAPEELDSVCLRSGLPRFKRPREYVFVKAIPRSASGKLLRRKLRTGEYE
                     TFEGNKVTVKEDS"
     gene            11705..12517
                     /locus_tag="VM910_07225"
     CDS             11705..12517
                     /locus_tag="VM910_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI62369.1"
                     /translation="MSKDYAEQRAAILRDLDGLHYEVDAKKKVGFLWLDRPPLNVVSY
                     RGRSQIAAIIEAFGQDPDVRVVVIRGKNGVFTSGGDVKAFPRIERDGMSHLAWNIAAA
                     ERCPKPVVCVLEKYAMGVGLELAMACDIRIATKDTVLALPEVTLGQIPGSGGSQRVAR
                     IAGLTRAVDMMMLGRRIPAPEAHSWGLLTRIADDTAALDRILDDIVTTLTTMSPLALK
                     TVKRVLNTSYDTSLSAGFEVEGHAYEKLRESGDYKEGIAAFTEKRKANFTGA"
     gene            12545..13732
                     /locus_tag="VM910_07230"
     CDS             12545..13732
                     /locus_tag="VM910_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62370.1"
                     /translation="MLNNTATVLSSSALIAFAFSLGLTAHAAEAIKVGVVTPLSGSYA
                     PIGKQVRWGAELAVKEINAAGGVGGRSFELLFEDEEANPPVAVRKSEKLLQQDKVDLL
                     TGTVNSGSTLAVGQVAERNNRILVTTVSYAPSITGAQCNPNVFRVNANAFMQSSALTS
                     WLTKNVSGKRYFFVGPDYEMGRSSISAFQDDIKRLGGTDVGSAFPPLGAKDFTPYVGQ
                     IRSARPDVIMTATAGNDTVRLLTQLKEYGILSNKLTLAGSAGAVTQENVGAMDGAAEG
                     FLSAAGYSIDIDTPANKKFVAAFKKEFQSDPDLFAADTYGLFYLFKRAIEKAGGTDEQ
                     KLRAAMEDATWQTPQGTKKMRKGDHQAIVDMVVVKVTGNDFHTVGKVPGEDAVGPDKC
                     EKF"
     gene            13761..14654
                     /locus_tag="VM910_07235"
     CDS             13761..14654
                     /locus_tag="VM910_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62371.1"
                     /translation="MEDLLGYLHVVVLPQVIDALIIGVALALVALGLTMIFGLLDVIN
                     LAHGDLYMLGGYAAFTVLGWGAGYWSALVLVPILVGAVGWALEELGIRPLLPRADRAI
                     VTLLLTFGVSLILRDIAQVVWGTETHAVAPPFTGIVSFAEVFVPTYRLFVFGIALIAI
                     FATWWLVYRTRLGAVLRATAVDPSMVASFGIPIRLVYGLTFVYGCGLAGLAGVLLSPI
                     YAVFPTMGHDFLVMAFAVVIVGGMGSIIGAVVAAVLLAQVQGLASLWIPPVWAETLVY
                     GVMLLVLVARPGGLFNRLGEA"
     gene            14654..>15025
                     /locus_tag="VM910_07240"
     CDS             14654..>15025
                     /locus_tag="VM910_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62372.1"
                     /translation="MLQPNVPPVEPQPSLHAAARVTVGDRLLQALYPPQSSAAQLRWV
                     GFAMLALAAIFPWLGGPFYTRLAIESFLFGAIALSVDILLGYAGLLSLGQAAYFGLAA
                     YISALMYLHVTQSFWLVLLAVV"
BASE COUNT         2771 a         4642 c         4697 g         2915 t
ORIGIN      
        1 gccggattga tccccatccc cctcgtcgac atggctgcag ttggcggagt tcaactccag
       61 atgttacgcc gcctttccga aatttacggc gttccatttt ccgaaaatcg aggcaagtcg
      121 atcatagcta gcttggcagg agcggtgatc ccggcgagca ctgcgacgac caccgcggtg
      181 ggcgtcggca gcctcgtgaa gagcttgccg ggaataggaa cggcaatcgg tgcgctgacg
      241 atgccggtgt tctccgccgg cgccacctac gtgatcggca aggttttcat ccagcatttt
      301 gcttcgggtg ggacgctgct tgatttcaat ccccccgatt atcgtgagtt catcaaggcc
      361 cagaaagaaa agtttgccgc ccgctcggcg gcagcgccat cctccggtcc ttccgcttcc
      421 gccgcccgaa gtgcgtcgag caagggcggc gccgcaaccg gcggcccttg atcgccatgg
      481 gcttttaatc cataccgacg tcggtacgga ccggccgggc gctagaggta gagacagaga
      541 tcatgacgta atgacggtgg cgatcgctgt tataatctac atcgtattct gcattctcac
      601 ggggctctgc ggcagccaac gccgcatggg gtttttcggc acattcataa tgtcgctttt
      661 cattactccc attgtgatga taatcgtgct gaaattaacc gcgccatcag aacgcgtcga
      721 atggcgtcac cgcccgcaga gcgattgact gccgttgcac tagaagcggc agtttctctg
      781 cggtcgtaag gattgacgcg cgacgcgtgc aaacgggcac atccagcgat gtctttcttc
      841 acgctcgttc gccgtgaaat gtacggctcc ctgaatcggc tgatcgtcat atccgccctg
      901 ggaggcatta gcaccgccgc gattctaacc gcgatcaatt ccggggccca ggccgctgac
      961 agcggcaagg tcagcctctg gtcagcggcg cttttcattg tcgcgctttt tctcttcatc
     1021 aagacccagc attacatttt gatcacggcg acggcggaga tcgaggccat catccacaaa
     1081 ctgcggttgc gcctgatgga tcacgtgcgc cactccgagc ttcttccgct ggaggcgatc
     1141 ggccgtggag aaatcgttgg cgccatcact aaagagaccg cgacactcac gcaaatgagc
     1201 aacacgctag cttttgcggc ccaaggcgcg gttctcatct tcttcgttgc aatgtatgtt
     1261 gcgtatctgt cgctcctcgc cttcgcgcta agcgtcataa tcctcggcat cgcagcagtg
     1321 ctgttccacg caaaaagccg ccaactccgt atggcgtctc acgaagcgtc taatttggaa
     1381 caccgactgt tcgatcgtct gatggacctc ctgaacggct tcaaggaggt gaggctcaac
     1441 agcgtgcgta gcgacaccct atttaacgat gttgtcgaaa tgtcgagggt tgccgccaac
     1501 atcaaaatcc gcgcccagag cgaaaccttc aggcgaatgg tgttcttgca aagcgcgctg
     1561 tacggattgc tcggcgctat agcgttcgcg gtcccgactt tcagcggcgc gatgggagca
     1621 gaaaccgtca cgaagacgat aacggctctc gtgtttgtcg tcggtacgtg ttttggcttg
     1681 gttcaggcga tacccatttt gagcgccgca aatacggcag cggacaacat cgagcaactg
     1741 gaggctaggc tactggcgac tatccccatg acggaagtca ccgcggttga gccctcgaag
     1801 cgcttccaca agatcgagat gcgcaatgtc gtgttccgct acgtcgacaa atggtccgac
     1861 gccgttttcc aagtcgggcc gatcgacttc acgttgctat caggcgatct cgtcttcatc
     1921 accggcggca atggttcggg caaatcgact ttcctgaaat tgctcgccgg gctgtataag
     1981 cccgattcag gcgaaatcac ttttgatgga gtgcgcgtca acgacagcac gcgtcaggcc
     2041 tatcgcgagt tgattgccgc gatcttccct gactatcatc tcttcgagaa actctatggg
     2101 gttcccgatc ccgatccagc ggaggtcgac agtctgctgg cgcaaatgcg actgcgtgac
     2161 aagacgcacc tcactgacgg ccattttagc acgctcgatc tatcgcaagg gcagcgcaaa
     2221 cgtctggcgc tcattgtaag cttgctggag aagcggccgc ttctgttgct ggacgaatgg
     2281 gccgccgatc aagacccgga atttcggcgt aaattctact atgatctgtt gccggaattg
     2341 aagcggagcg gcgagacggt ggtagcgata acgcatgacg accgctatat cgacgaaatg
     2401 gacttgcccg ctcgcagact gcgcatggaa gacggccggt ttgctgcgca gcagacggtg
     2461 gagaacaggt gatgaaagcg tatctgtggt cagatgggga gccgcccccg acgacgagac
     2521 gagcccggtg gcggcggttc gccgaacgcc atctcccgag tgtagtgatc tacctgatgg
     2581 tcgcgacact cgtggcggtg gtgctctacc ctcacatggt ggtaacggtt cccagcggct
     2641 acgtcggcgt gctgtggaaa cgctttggcg gcggcacggt gctcgatccg cggcggttga
     2701 agaatgaggg tttcaatctc attttgccct ggaaccaagt atttctgtac gacctgagac
     2761 tgcaatcctt taccgagtcc tacaacgcca tctccagcga cggcgtgagc ctgacggcga
     2821 ccatcattgt ccggttccgg ctccagcgcg atgcagtgcc cgtgctgcac caagcgatcg
     2881 ggccaaatta cgagcaagtg ctggtgcagc ccgggatcgg cagcctgacg cgcgaggtga
     2941 ttgcccaata cactgccgaa caggtctatt cgacggcacg ccaagaaatt caggacaaaa
     3001 tccgcagcct ggtggaggac aggctatccg agaagatgat ggagcacgaa ggcgaagagt
     3061 cataccgcgt ctcgatgagg gacaccatca tcctttacga catactcgtg accggcattg
     3121 agctgcccgc ggccatcgtc gccgccatca atcgcaaaac cgagcaatac tacgttgcgg
     3181 aggaatacaa gtttcgcgtc gagcgcgaaa aacgagagtc ggaacgcaag aagatcgagg
     3241 cagaaggcat acgcgatttt caacagaccg tcagccaagg catctcggag tcctacctgc
     3301 gttggcgcgg catcgaggcg acgctgcagc tctcgcaatc caccaatgca aaagtcgtcg
     3361 tcatcggcag cggcaaagac ggcttaccga tcatccttgg caacgtggac accccggcac
     3421 cgcccgcggg gagcacgtcg ccgagcggga ccgacacgat gaacaaggaa agcaccacgg
     3481 ctgccggccc ggcggtgccg ttggagaaaa cgcccgctgc cggcttggcg acgccgaccg
     3541 aaaaggtagc gcctgccatt tcgggtaatc cgtctgctgc tcccgaggca tcgcgctcgt
     3601 tttggccgct cactctctcc gatatcgaga cttatcttta ccggatccta cgtccggagc
     3661 cgaacccaga gccgaaagcg gcggagcctc gctcaagtca gccgtccgag agaccggtcg
     3721 cagcgcagcc gcggtagcgt cggcctgcga ccggcattcc cgagatcgca cggcggcggg
     3781 gaaagctcca atcgaaactt cgcagcggca gttcaggtgc cgcgacccca cagcccgcaa
     3841 tagcgccgag ctacgagtgg caggccgttc tcatagcccg gccaatttgc gtatttcggc
     3901 agcttaaaat ccttctcggc tggctcccag catttgccgt cttgccccat cttcttcatc
     3961 tcggcggacg cctcctgcat cagcgtgagg atgtcttgcg catccttctt ggtaccgagt
     4021 cgaccacccg gctggccggg atgtccaggg atcagcctct cccagtccag cgcgaggacc
     4081 ttcttgatga actcctccgt ttcgagcgga tagatgtcga tgaagccgcg cccgggaaac
     4141 gatccgacgg gtatcgtgtc gacaacgaag acaatcttct ccttcggcaa ccacatcact
     4201 aagttcgagt cggagtgatt gaggccgaaa tatttcagct cgagcgtagt gccaccgagc
     4261 ttgatcgtgc gaccggcgcc gctgactact tgatcgggga gcggagtatt cggatccttg
     4321 agcacggcga ggcgctcctt ggcccgcctg tgcgcaatga tggtggcccc ggcgtccttg
     4381 aacggcttgc cgccggcgat gtgatcgtag tggtggtggc tgtagatcag gaacttgatc
     4441 ggcttgtccg tcaccttctt gatctcggcg atgtaatctt ggccgccggt cggccggccg
     4501 taggcgatcg gatcggtcgc gatcacgccg tctttggtga cgatgaacat cgattgatgg
     4561 ttgccgttgc ggaatatgta gacgttgtcg gtgccgtcga ccttggtggt ctccatttgc
     4621 ggccgttgtg cctgctgcgc cagccccgtt gttgcgaacc cggcggcact caacgcgacc
     4681 gccatggctg caagacgaat ctgcgacata atgattcctc cctcgtgctt ctcgggccgc
     4741 gtcgtccatt ttcgctgcgc cgccctgctg cgagttatag tatagacgcg gacgagatac
     4801 cacgtatcga acgttagttg cggccctcac cgaaggttgg ggaggaaggc cagcgccacc
     4861 aagtgtgcgg cgatgcaaaa tcgcaattca acgacccgac cgttacgcac gatcgctttg
     4921 cgctcggcaa aactaacttg cacgtcgtga tggtcgagga aggtttggtc gagcgggtac
     4981 actgccttgt ataccgcctc cttggcgacg aaaagcagtc gtccatgata agggtcatca
     5041 cccagtctca gccgctcttg cggcgttgcg accaagtcca gcaattcaga cggcaatgat
     5101 tcggccggct cgacgtctat tcctacggcg cccacatcac gacccatccc gacggctgcc
     5161 accgcaaccc gagaatcgtg ggcgagcgaa ccgaccacgc ctgcgggcca ggctggcgct
     5221 cccccggacg ttctcggtaa cgcgcattcg ggttgtccca atcgcttcag gagttgacgc
     5281 gctacgatgc gagccgcgcc actcgcacgc cgcaccttga cgacactcaa ggcgaaggca
     5341 ggcgcctcct ccggcaacaa cgcatcttcg tcgccaggtg agatcaagcg atggccaatc
     5401 atgatgcctg gaaaagagag ggcatcgatt gctgattgaa gcgagggatc gacggtcacg
     5461 atcattctcg ggggcaattc tcggccttgg tggatcgtgt tcgttcgagc cacccaacac
     5521 gcagcacgcg gatcaacaat tcttcctgca atcgaccgct cgcatggcga cgccgtaaca
     5581 ccagcctcga cgcgatgtcc aactgatgct gatgttgccg cctcgacggc tgcctgcgct
     5641 ccggcgcgcc ctctataacg gcaaatgatt ttgctcgttg tcaatcttgt cgtgacgacc
     5701 cgcctagtcc tatagatcat acaacgagac gaaacggccg aaagggcggg ggctaacgct
     5761 tttccaaggt ggaccgcgtc ttgctggaag caatcggaac cgtgtcgcag cgatgggtcg
     5821 gctcgcctgt tgcgcgcaag gaggaccagg cgctgctgac cgggcaggcg cgcttcatcg
     5881 acgatctctc gccaattccc ggaatccgtt tcgcagcgat cttgcgctcg ccccatccgc
     5941 atgcgcggat cgggcggatc gatttcgcgc gggcgcggga cctgccaggc gttcggggca
     6001 tcgtcaccgg caaggacgtc gcggatctga tcgggccggt gccaagcgtc gtgaaggctc
     6061 cgctcgccta ctatcctgtc gccatcgacc ggacacgata cgtcggcgag cccgttgccg
     6121 tcgtggtcgc tgatacccgt tacatcgcgg aagacgcgtg cgatctgatc gaggttgagt
     6181 acgacgtgct tccggcagtg tcggacctgc gcagcgccac tgcgcgcgat gccccggtga
     6241 tccatgagat ggccggatcg aacgtcataa gccggcgcaa cttcatttac ggcaacccgg
     6301 atgctgcctt tgccgcggct gagcgggttt tcgagttctc gtactcgtat ccgcgctatg
     6361 cctccacgcc gatggagacg ttcggcgtca ttgcgcactt cgaacgcgct cccgaccgtt
     6421 acacggtgtg gtcgaacttc caggggccgt tcgtggtcca gccgctcatg gccggcgcat
     6481 tgcgcgtgcc cggccaccgg cttcgcctca tcacgccacc gagcagcggc ggaagtttcg
     6541 gcatcaagca ggccgttctc tcgtacatcg ttcttctggc ggccgtcagt cgcaagacgg
     6601 gcgtgcccgt caaatggatc gaagatcgcg ccgagcacct cacggcctcg tccgcctcga
     6661 gcgatcgctt gggaacggtc gcggccgcgt tcacgaagga cggcgagctc acaggattgc
     6721 gattcaagaa caccgcgaac atgggcgctc acgttcgccc acccgagccg gcctccctct
     6781 atcgcatgca cgccgcttcg aacggctgtt acaaggtgcc caacatttcc atcgacaacg
     6841 agctcgtcgt cacgaactgc acgcccgtcg gtctcaatcg tggatatggc gggccgcaat
     6901 tctattttgc cctcgaacgt atcatggaca tcgcggcacg cgggctcgga atggatccgg
     6961 cggagctgcg ccgccgcaat ttcattcctg ccgatgtctt cccttacaga gcgccggcag
     7021 gcgccgtatt cgatgccggc gactacgatg cggcgctgtc ggaactcctg cggatcgcgg
     7081 attatgacga actcaaacgc cggcgcgacg aagtccgccg cacgggaggg cttttcggca
     7141 tcggcctggc agcgggtgtg gagccgtccg gctcgaacat ggcctatgtt tcattggcgc
     7201 agacagcgca agaccgccgc cgcgccgacc gaaagtcggg cgccaacgcc agcgccgtag
     7261 tcacggtcga tccgagcgga caagtcacgg tgcgcctgtg cagcaccccg aatggccagg
     7321 gccatgcgac ggttgcggcg cagatcgtag ccgacgcgct tggactgcat cccaacgata
     7381 ttgatgtggt gacggagatc gacacgctga cgagcccttg gtcgattgcg tcgggaaact
     7441 attcaaaccg cttcgcggca atcgtcgtcg atgccgtcgc gaagtgcgcg aagcaagtcg
     7501 cgcagaaaat caaattgctc gccgctaacg ccctggaggc tgctccggaa gagatcgagc
     7561 tgcgcgaagg ctatgcccga gtgcgcgggc aatccaacaa gggactgccg tttcgcaagg
     7621 cggcggcgcg cgcgcactgg gacccggccg gtttgccgga caatgccact ccgggaattc
     7681 atgaagctgc catcgtgtcg ccgccggtct tgggctctcc cgatgacgaa gaccggattg
     7741 cctctgccgt cacattcggc ttcgtgatcg atctggcggc agttgaaatc gatcgcaaga
     7801 ccggggagat ccgcatcgac aaatatgtgt cggttcacga cgtcggaacc cagctcaatc
     7861 ccaaaatcgt cgaaggacaa gtccacggcg gctttgccca cggtctgggc gcagccctgt
     7921 tcgaagagtt ggcgtatgac gagcggggca atttcctgtc ggggactttt gccgattatc
     7981 tttgcccgac agctgtcgag gttccacgcg tcgagatcgg ccacgtcgaa acggcctctc
     8041 ccgtcaacgc gctcggcgcg aagggaatgg gagacggcag ctccatgctc acgcccgctg
     8101 cgatcacaaa cgccgtcgcg gatgcgctcg gacgcgacga tatttcgttg ccgttgaacc
     8161 tgcggcgcgt ttgggcattt gcaaacggcc atgaggttac accgcgcccg atggcagcgc
     8221 aggcccgagc ggagcggacg gccgtcggcg gtgcgctgac tgggagcgga gaagtctctc
     8281 tctccgcccc cgtggcggag gtttggcggc ggctcctgga tccgagcgag cttgctgcca
     8341 tcatcccggg atgtcggggc ctcacccagg acgatccgga tcgttactcg gctcaggtcg
     8401 tgatcggcgt tgccggcatt cgtggcacct atcacgtgca aatcgagatg cgcgacaaga
     8461 aggagaaaag ctctgtgcgc ctggtcggca aggccttcgg ggcattgggc tttggatcgg
     8521 gatcggggtt cgtgagcctg caaccggagg ccggcggtcg cacgcgcctc cagtatcgct
     8581 acgaagccga tgtcggcggt aaggttgcag ccgtcggcca gcgcatgctc ggtagcgtga
     8641 cccgttatct catcgctcaa ttctttcact ctctggagcg ccggctaaga cccgcgggtg
     8701 gcgagggctg gcggacttgg tggtcgagat tgcgacggca tgggcgaaac ggaggcgggc
     8761 cgtgaagcct gtggcgttcg actattatcg gccggaggcc gtttcggaag cgctcgctct
     8821 tgtgtccgag atgggcgatg aagcggctgt cctcgcgggc ggcatgacgc tcgggccgat
     8881 gctaaacctc aggatggttc gcccgcgcgc cgtgatcgac atctcacgca tccaggcgct
     8941 gaaggccatt tcagcgagat ccgacgtcgt ggtgaccggc gccggagtgg tacagggcga
     9001 cgcgctgcgt tcggacgtca tcgcgcgggc ggcgccgctg ctggtcatgg cgttgccgtg
     9061 ggtcgggcat ttccagaccc gcaaccgcgg aacgctcggc ggttcggtcg cgcacgcgga
     9121 cccgagtgct gaaataccac tcgctctcgt cgtgtgcggc gggaccgtcg tgctgcaatc
     9181 gcgccggcgg caacggcgcg ttcccgcgcg ggagttcttt ctcggcacgc tcacgacgca
     9241 gcgccggccg gacgagatcg tgacggcgct ggaatggcca cgcgctgcgc ccgatgccgg
     9301 tcacgcgttc accgagatcg cgcagcgcca tggagatttc gcgatcgcgg cggcgggctg
     9361 cagcgctcgt ctcgatgggg ccgaccggct ggaggaactc tcacttgggg tcgggggggt
     9421 ggagagccgg ccagtcgcga tcgacgtgaa gagctttctc ggccgcccgg ccggcgaaat
     9481 cgcggccagc ctggccgagc atgccgccgt cagcgtgaca cccatggagg atcactcggc
     9541 aagcgccgat tatcggattg cgctgacgaa ggtgctggtg gagcgcgcgg tcaacgctgc
     9601 gctcgaaatg gcccggcagc gaaggggccg cctgcaatga caacgcttta tcgggcagcc
     9661 ggcaaccgct tcacggtcga attgaccttg aatgggcagc gccgatccgg cgaggccgag
     9721 cctcgcatgc tgctgtgcga ttttctgcgt catgagctgc aggccttcgg ggttcacgtc
     9781 ggttgcgagc atggcgtatg cggtgcctgt acggttctgg tcgatgggcg agcagtgcgc
     9841 agttgcaccc agtacgccgc ccaagcggac ggtgccgaca tcgtcaccgt ggaggggctc
     9901 gctgaaccgg gcacgctcaa cgaggttcag cgcgccttcg cgaagcatca tgctctgcaa
     9961 tgcgggttct gcacaccggg gatattgtgc tccgtgacgc atttcctgcg tgataccccg
    10021 ggaccgaacg agcgggaggt ccgcgagatg ctttcgggtc acatctgccg gtgcacggga
    10081 tatgcgtcga tcgtcgccgc ggtactcgaa gccgcagccg tcatcggcaa agggtcgcga
    10141 ccatggatct cggcagcgca tttgccgccg cggtgatgcg ccaacctcgt caggaggcgt
    10201 tggtcgaagg caaccgtcgg atgacctatg tcgattggta tcgcgacatc gcgtcggttg
    10261 ccggcggctt gcaggacacg gggttgaggg cgggcgatca tctcgtggtg gtgatgcgga
    10321 atcggtacga gatggcgacg ctgtactggg catgccagct gctcggcatc gtcttcacgc
    10381 cggtaagctg gcgcgcgacc gcagacgaga tcgggtattg cctcgaggac gccgaagcgg
    10441 cggccgtcgc gttcgacggc gcctcgggct ctgcggccgc cgaggctgcc gcgacgctga
    10501 agatcgaccc gcgacgtgtg atcgttgccg ccgacggcaa aggcgagggt atgccattcg
    10561 gacgactgcg cgattcagcc gcggttgccg gtcccgccgc agtcggcgag tcctcgatat
    10621 gcttgatgct ttacacttcc gggaccaccg ggcgcccaaa aggcgtgccg cgcagccatc
    10681 acagcgagct gatggccgcg attgctcaca ttgctcagaa ccagtatcgg cggggggatt
    10741 ccagtctcgg cgtgatgccg ttcttccaca ccatgggcgt gcgaattctc ctcagcacgg
    10801 cgctgctcaa tggccgattg gtctgtgtgc ccgaatatgg acccgaggag gttctacggc
    10861 tgatctccga agagcggctg acgacgctct tcctcgtgcc cacgatgttc cacgacgtgc
    10921 tgcgacaccc caagttcgat tcctatgact tgcgttcctt gtcccgcgta ggcttcgcag
    10981 gcatgacgat gacgccagcg ctgatcgagc gctgcctgga tcagctcaag cccgatctgt
    11041 tcgtcaacta ctacggttcc agcgagatct acaccttcag ctactgcgac catctcggcc
    11101 gcaagcccgg ctgcgccggg cgcgccggga tgaaccagat catccgcgtc gtttcgccgg
    11161 cccgcgacga cgatatcgag atcgatctgc agccgggcgt cgccggcgag atcgtcgcca
    11221 gcatgcgcag ccccgaggca ttttcgggct actggaagcg ccccgacgcc gacgccaagg
    11281 cgatccaagg tcgctggtac cgcaccggcg atctcggaaa gctcgacgag gatggcgagc
    11341 tctacgtcgt cggacgcgtc gacgacatga tcatcagcgg cggcgaaaac atctttcccg
    11401 aggaggtgga ggacaccctc gcgcgcagca agctcgttgc gggatgcgcc gtcgtgggaa
    11461 tgccggatga gcgactcggc gccaaggttg tggccttcgt cgaaccggcg gtcgcccagc
    11521 tcgcacccga ggagctcgac agcgtgtgct tgcgcagcgg actcccccgc ttcaagcgcc
    11581 cccgggaata cgtattcgtg aaggccattc cccgctccgc atccggcaag cttttacgcc
    11641 gcaagttgcg aaccggggaa tatgagacat tcgaaggcaa caaggtcacg gtaaaggaag
    11701 actcatgagc aaggactacg ccgaacagcg cgccgcaatt ctgcgcgatc tcgatggcct
    11761 gcactacgaa gtcgacgcga agaagaaggt cggctttctc tggctcgatc ggccgccgtt
    11821 gaatgtcgtg tcctatcgcg ggcgcagtca aatcgccgcg atcatcgagg cattcggcca
    11881 ggacccggac gtgcgcgtcg tcgtgatccg cggaaaaaac ggcgtgttca cgtcgggcgg
    11941 cgacgtcaag gcgttcccgc ggatcgagcg cgacggcatg tcccatctcg cgtggaacat
    12001 cgccgcggcg gagcgttgcc cgaagccggt cgtttgcgtg ctggaaaagt atgcgatggg
    12061 agtgggcctc gaactggcca tggcttgcga cattcggatc gcgaccaagg acacggtcct
    12121 ggcgctcccg gaagtgactc tcggccaaat tcccggcagc ggcggtagtc agcgcgtcgc
    12181 gcgcatcgcc ggcttgaccc gcgcagtgga catgatgatg cttggccgcc gcatccccgc
    12241 gccggaagcc catagctggg gcttgctcac gcgcattgcc gacgacacgg cggcgctcga
    12301 tcgcattctc gacgacatcg tcaccacgct caccaccatg tctccgcttg cgctcaagac
    12361 ggtcaagcga gtgttgaaca cgtcgtacga cacgagtctg agcgcaggct tcgaggtcga
    12421 ggggcacgcc tacgagaagc tgcgcgagag cggggactac aaggaaggca tcgcggcctt
    12481 caccgagaag cgcaaggcca atttcactgg cgcgtgaacg tcagagttgc aaccggagga
    12541 aatcatgctc aacaacacgg caactgttct aagcagcagt gcgctcatcg cattcgcatt
    12601 cagcctcgga ctgaccgcgc acgcagccga ggcgatcaag gtcggggtgg tcacgccgct
    12661 ctcgggcagc tacgctccga tcggaaaaca ggtgcgttgg ggcgccgagc tcgccgtcaa
    12721 ggagatcaat gcggcgggcg gtgtcggggg tcgttcattc gaacttctat tcgaggacga
    12781 ggaggccaat cccccggtcg ccgtgcgcaa gtccgaaaaa ttgctccagc aagacaaagt
    12841 cgaccttctg accggaaccg tcaacagcgg atccacgctc gcggtcggcc aagttgcgga
    12901 acgcaacaat cgcatcctcg tcacgacggt ctcctacgcg ccgtcgatca cgggcgcgca
    12961 atgcaatccg aacgtttttc gcgtgaacgc caacgccttc atgcagtcga gcgcgttgac
    13021 ctcatggctg acgaagaacg tgtcgggtaa acgctatttc ttcgtcggtc ccgactatga
    13081 gatgggccgc agcagcattt ctgcgtttca agacgatatc aagcggctgg gcggcaccga
    13141 cgttggaagt gcgttcccgc cgctcggagc caaggacttc accccatacg tcggccagat
    13201 ccgatctgca cgtcccgacg tcatcatgac cgcgacggcg ggcaacgaca cggtgcgcct
    13261 tctcacgcaa ctcaaggagt acggcatcct ctccaacaag ctgacgctcg cgggctccgc
    13321 tggggcggtg acacaggaga atgttggcgc gatggacggc gcggccgagg gtttcctgag
    13381 tgccgccggc tattcgatcg acatcgacac gccggccaac aagaaattcg tggccgcgtt
    13441 caagaaggag ttccagagcg acccggatct cttcgctgcc gatacctatg gcctcttcta
    13501 tctcttcaag cgcgccatcg agaaggcggg cggcaccgat gaacagaagc tgcgagccgc
    13561 gatggaggac gcgacctggc aaacgccgca aggcacgaag aagatgcgca agggcgacca
    13621 ccaggcgatc gtcgatatgg tcgtggtcaa ggtgacggga aacgatttcc acaccgtagg
    13681 aaaagtgccc ggcgaggacg cggtcggtcc cgacaagtgc gagaagttct aagccaaaac
    13741 cacgagcgcg agctgcgccg atggaagacc ttctcggtta tctccacgtc gtcgtcctgc
    13801 cccaggtgat cgatgcgctg atcatcggcg tggcgcttgc gctcgtggcg ttgggcctga
    13861 ccatgatttt cggcctgctc gacgtcatca atctcgcgca tggcgatctc tacatgctgg
    13921 gcggctatgc cgctttcacg gtccttggtt ggggcgccgg ctactggagc gcgctggtgc
    13981 tggttccgat tctcgtcggc gccgtcggct gggcactgga agaactcggc atccgaccac
    14041 tgctgccgcg cgccgatcgc gccatcgtga ccttgcttct cacgttcggc gtcagcctca
    14101 tccttcggga catcgctcag gtggtgtggg gaacggagac gcacgccgtg gcgcctccgt
    14161 tcaccggcat cgtgagcttc gccgaggtct tcgtgccgac ctaccgcctc ttcgtcttcg
    14221 gcatcgctct catcgcgatc ttcgccacct ggtggctggt gtaccgcacg cggctcggcg
    14281 ccgtcttgcg cgcgaccgcg gtcgatccgt cgatggtggc ctctttcggg ataccgatca
    14341 ggttggtgta cggcttgacc ttcgtctatg gctgcgggct tgccgggctt gccggcgtct
    14401 tgctcagccc gatctacgcc gtgtttccca cgatggggca cgactttctc gtcatggcct
    14461 ttgccgtcgt catcgtcggt ggaatgggaa gcatcatcgg ggcggtcgtc gcggccgtgc
    14521 tgcttgcgca ggttcagggc ctcgccagcc tctggattcc gccggtctgg gccgagacgc
    14581 tcgtctacgg cgtgatgctg ctcgtgctcg tggcgcggcc aggcgggctg ttcaaccggc
    14641 ttggagaggc gtgatgctgc aaccgaacgt gccgcccgtc gagccgcagc cgtcgctcca
    14701 cgcggcggcg agagtcaccg tcggcgatcg tctgttacag gcgctgtatc ccccacagtc
    14761 cagcgcggcg caattgcgct gggtcggctt cgcaatgctc gcgctcgccg caatctttcc
    14821 gtggctgggt ggtcctttct acacgcgtct ggcgatcgaa tcgtttctgt tcggcgcgat
    14881 cgccctcagc gtcgacattc tgctcggcta cgccggactg ctgtcgctcg ggcaggccgc
    14941 ctatttcgga ttggccgcct acatctcggc attgatgtat ttgcatgtga cccagtcgtt
    15001 ttggctggtg ctgctcgcgg tcgtc
//
LOCUS       DATLJT010000321         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1167068,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000321 DATLJT010000000
VERSION     DATLJT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167068"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VM910_07245"
     CDS             <1..366
                     /locus_tag="VM910_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI62373.1"
                     /translation="MTRVFDQFPRLAERRRQAAGTLSGGEQQMLAIGRALMSQPKLIM
                     FDEPSLGLAPTIVERTFDIVTTIRASGTTVLMVEQNASAALDMCDHAYLLESGSVAIE
                     GSGRELIDDPHVREAYLAG"
     gene            complement(434..1417)
                     /locus_tag="VM910_07250"
     CDS             complement(434..1417)
                     /locus_tag="VM910_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62374.1"
                     /translation="MQAIRLKALGVIIAVVAAVVAALGQPAAAQDKVKVGVFPTASSL
                     PYFVAIERGFFKEQNIEPETIRLIGGPPNIAAMISNQIEAAVVLVTIEAMNANLKKPG
                     VAMYIGVHSQSKIYQMEQFVVRTGYAAATLKDLKGARIMSAPGPANLVAAKAVLAKVG
                     LNEGDYTIDQLDMSQHVNVMTSGTFDAGYTLEPQASTMRKLGVARTLEAGVIAKYILG
                     DEDGNAFAAGCAITSDFIKSRPDVARRFAAVWKKGIDFVNQNPQEARKYLAKNTLTPE
                     DVVDTVPMVRYFMAGELTDKHKAEFQKFIDFSTSVGTLPEKVDITKYLQAY"
     gene            1632..2318
                     /locus_tag="VM910_07255"
     CDS             1632..2318
                     /locus_tag="VM910_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62375.1"
                     /translation="MNRLRQWRLVGSAVGGERHFWLERLIFGLDGVLRRWQSVVEYTH
                     DPACILRIKFGRLDQDFTLSDGTAGHAGERFIDLHLWNEHVPAMPKEGASIAWARQMS
                     LCFQHSLRQLARYLATRPDLDDISLVRCTMEFAGQERNDQMVRLIGRYGFEFVAPTTT
                     VTMGERARRLVENIFISLIVLARNQAALRRDTLRRGRTRAFMSRKVLEQRYGRGADSV
                     DLRHRPICPS"
     gene            2479..3219
                     /locus_tag="VM910_07260"
     CDS             2479..3219
                     /locus_tag="VM910_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI62376.1"
                     /translation="MLFRVGRAFAVVSCVSVVAIEFAAAADIPYVQSPAPVDVPPSWA
                     GFYLGGQIGYGTDSVRWRNLGASAFFSPPNSLTRDRGSGVIGGGQLGYNFQYNRLVFG
                     IEGSVSAADFDRSFASPYFPATDVWSSKLTWLTTVTGRVGYGFDTWLPYVKGGFAAGN
                     VETSIQSNPFGVMSQGSSVHSGWTLGGGVEFKVAPQFSLGLEFMHTDLGRSNDISGPQ
                     IVPGAPESYGVGLRSNSIMARFNYLFGR"
     gene            3412..3488
                     /locus_tag="VM910_07265"
     tRNA            3412..3488
                     /locus_tag="VM910_07265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3446..3448,aa:Met,seq:cat)
     gene            complement(3821..>4048)
                     /locus_tag="VM910_07270"
     CDS             complement(3821..>4048)
                     /locus_tag="VM910_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62377.1"
                     /translation="TARPSAHWIARQLTEAYGWQQAPQYIVRDRDCVYGDCRDPAASS
                     NGYTGSTDFAAVAMAKRIFGETHRFDPTGLS"
BASE COUNT          715 a         1206 c         1284 g          843 t
ORIGIN      
        1 atgacgcgcg tcttcgatca gtttccgcgg cttgccgagc gccgccggca ggcggcggga
       61 accttgtccg gcggcgagca gcagatgctt gcaatcggcc gagccttgat gtcgcaaccc
      121 aagctcatca tgttcgacga gccttcgctc gggcttgctc ccacgatcgt cgagcgcacg
      181 ttcgacattg tcaccaccat tcgtgcgtcc ggtacgaccg ttctcatggt ggagcagaat
      241 gcctcggccg cgctcgacat gtgcgaccac gcttatctcc tggaatcggg ttccgtggcg
      301 atcgagggaa gcgggcgcga gttgatcgac gatccgcacg tgcgtgaggc gtatctcgcg
      361 gggtgacgcc gggttgcgcg gctggctcct cggggagcgg cagctgcgga gcacaagttg
      421 gttcgcggat atttcagtac gcctgcagat atttggtgat gtcgactttc tccggcagcg
      481 tgccgaccga ggtggagaaa tcgatgaatt tctggaactc ggccttgtgc ttgtcggtga
      541 gctcgcccgc catgaagtag cgcaccatcg ggacggtatc gacgacgtcc tcgggagtga
      601 gcgtgttctt ggcgaggtac ttgcgcgcct cctgcgggtt ttggttgacg aagtcgatgc
      661 cctttttcca aacggcggcg aagcgccgag cgacgtcggg gcggcttttg atgaagtcgc
      721 tggtgatcgc gcagccggcc gcgaacgcgt tgccgtcctc gtcgcctagg atgtacttgg
      781 cgatgacgcc ggcctcgagc gtgcgggcga ccccgagctt gcgcatggtc gaagcctgcg
      841 gttcgagggt gtagccggca tcgaaggtcc cgctggtcat gacattgacg tgctggctca
      901 tgtcgagctg atcgatggta tagtcgccct cgttgaggcc gaccttggcg agcactgcct
      961 tggcggcgac caggttggcc ggtccgggcg ccgacatgat gcgcgcaccc ttgaggtcct
     1021 tgagcgtcgc ggcggcgtag cccgtgcgca ccacgaactg ctccatctgg tagattttgc
     1081 tctggctgtg cacgccgata tacatggcga caccgggctt cttgagattg gcgttcatcg
     1141 cctcgatggt cacgagcacg actgccgcct cgatctgatt gctgatcatc gccgctatgt
     1201 tcggcgggcc gccgatgagt cggatggtct caggttcgat gttctgttcc ttgaagaagc
     1261 cgcgctcgat cgccacgaaa tagggcagcg aggacgctgt cgggaacacg cccaccttca
     1321 ccttgtcctg cgcggcggcg ggctggccca aggcggcaac gacggcagca acgacggcga
     1381 tgatcacccc cagcgccttc aaccggatcg cctgcatcgc atcacctccg tcgttccatg
     1441 gcgccccaat atctcgccca cgctcgccgt agccgggggc gcggctcgac ccataaggac
     1501 gaggtcggtc accgtcacca acttcagcgt catggggctg tacgttgcgt gggggttggc
     1561 gcagattgga agccggcgca gattaataga ttgtaatatt gcagcaagcg tgccgttagg
     1621 catccggtgc catgaataga ttgcggcaat ggcggctggt cgggtcggca gtggggggag
     1681 agcgtcattt ttggctggag cggctcatct ttgggctgga cggcgtgctg cgccgctggc
     1741 agtccgtcgt cgaatacacc cacgacccgg cttgcatttt gcgcatcaaa ttcggccgtc
     1801 tcgatcagga ctttacgctt tcggatggca cggcgggtca cgccggcgag cgcttcatcg
     1861 atctgcatct gtggaacgag catgtcccgg cgatgccgaa ggagggtgca tcgattgcat
     1921 gggcgcggca gatgagcctt tgcttccagc actcgctgcg gcaactcgcg cgctatctcg
     1981 ccacgcgccc cgatcttgac gacatttccc ttgtgcgttg taccatggag ttcgctggcc
     2041 aagagcgtaa tgaccagatg gtgcgtctga tcggccgcta tggattcgag ttcgttgcgc
     2101 ccacaacgac ggtgacgatg ggtgaacgcg cccgccgcct ggtcgaaaac atcttcatct
     2161 ccctgatcgt gttggcgcgc aatcaagcgg cgctgcggcg agacacgctg cgccggggcc
     2221 ggacacgggc tttcatgtca cgcaaggtgc tcgaacaacg ctatggccgc ggcgctgata
     2281 gcgtcgatct acggcatcgc ccgatctgcc catcctgacc gacgaagcgc cgcctatgga
     2341 taggcgcgct gtccgcgcga agagccacaa actttattaa ccttgtcgag aaggagacgc
     2401 atcgaagccg cgctttcgcc cacgttgccg gcaagtttcc ggaaaatctc gtgttggggg
     2461 cgaccaggga gaggccatat gcttttccgc gtcggtcgag cgttcgccgt cgtttcttgc
     2521 gtaagcgttg tggcgattga atttgctgca gcagccgata ttccctacgt gcaaagtccc
     2581 gcacccgtgg acgtgccgcc gagctgggcc ggattctatc tgggtggcca gatcggctat
     2641 ggcacggaca gcgtccggtg gcggaatctg ggtgcgtcgg cgtttttttc gccgccgaat
     2701 tcgctgacgc gcgaccgagg cagcggagtg atcggcggcg gacaactcgg ctataacttt
     2761 cagtacaacc gattggtgtt cggtatcgag ggatcggtat cggccgccga tttcgaccgc
     2821 agcttcgcca gtccatattt ccccgcgacc gatgtgtggt cctcgaagct cacctggctc
     2881 acgacggtga ccggccgcgt cggctacggc ttcgacacct ggctacccta tgtcaaaggc
     2941 ggctttgccg ccggcaacgt cgagaccagc attcagagca acccgtttgg cgtgatgtcg
     3001 cagggatcgt cggtgcactc aggctggacg ctgggcggcg gggtcgaatt caaagtcgcg
     3061 ccccaatttt ccctcggtct ggaattcatg cacaccgatc tcggcagaag caacgacatc
     3121 agcgggccgc agatcgttcc gggagcgccg gagagctatg gcgttggact gcgaagtaac
     3181 agcatcatgg ctcggttcaa ctatctgttc ggccgatgac gcgacggcgc caaatctgaa
     3241 tcgctttgag tgaggcgaga ggcggcaggt gccagccaac catctgcgcc attcccgcgg
     3301 cttgctcggt ctatctccct cgactacata gccggtgccg gcccgcgagg tcgccagcgt
     3361 gcaaaaagct tgcgagcttc tcgggcggca ctgtaagtcg ttgaaattgt tggcggggta
     3421 gctcagctgg tcagagcggc ggaatcataa tccgtaggtc gccggttcga tcccggcccc
     3481 cgctaccaac ggaatcaata gcttactgcc caggggcacg actaatttcc ccgatgggta
     3541 aacattgggg taaacccccc gtgttcaaac tgtcatattc agccttgcct tcggccagtg
     3601 gcggccgccc ccttgtcctt gtcggaaatc tacgctctga aatattggtg gtgcagtccg
     3661 cccagaaccg gcatcgccag cgggagaccg acagtctgga cggcacgcgg gaccggcgcg
     3721 tccttgtctc agcgataggt gcgtccgagc ctcgttgtaa tatttttggt aggaattgag
     3781 cagatggcgc agatgccgtt cgccgaacac aacaacatgg tcaagacaat cccgtcggat
     3841 cgaaccgatg agtctctccg aatatccgtt ttgccatggc gaccgcggcg aaatcggtcg
     3901 atcccgtata cccattgctc gaagccgctg gatcacgaca atcgccatag acgcaatccc
     3961 gatcgcgaac gatatattgc ggcgcctgtt gccagccgta cgcttcagtc agctgacggg
     4021 caatccaatg ggcgcttgga cgtgcggt
//
LOCUS       DATLJT010000322        14370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2085902,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000322 DATLJT010000000
VERSION     DATLJT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2085902"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(648..1052)
                     /locus_tag="VM910_07275"
     CDS             complement(648..1052)
                     /locus_tag="VM910_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62378.1"
                     /translation="MSQIKCEAGHTQLEKVLIHARKETKLLPVSALVFVGDAMEENPD
                     TLAHEASELGRLRVPAFMFQEGNNHQVERVFRNIAELTRGAYGRFDSGSARQLAELLK
                     AVAVFATGGMAALAARQDAGAVKLLSQLRRPP"
     gene            complement(1063..1374)
                     /locus_tag="VM910_07280"
     CDS             complement(1063..1374)
                     /locus_tag="VM910_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62379.1"
                     /translation="MTSEQDKSSIINRGESAPARSELDAFLANIRGRTTAAAGTRGRL
                     IFALDATMSREETWDLACQLQAEMFREVATAGGLDMQLVLSGSDCENQREALHSPRLP
                     R"
     gene            complement(1550..3805)
                     /locus_tag="VM910_07285"
     CDS             complement(1550..3805)
                     /locus_tag="VM910_07285"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HVI62380.1"
                     /translation="MAGIPFMLTNAMKDALRSRGLIDDEIEKTTPAEAQKILLTPDPR
                     AVRESIQAIATQAKAAAGATPGLLQLSRLHPVSETLVPSRYTLDDIDRMISAAIADCE
                     AGHNVYIEGRLVPASLRGSERGTLKDTVAVFALVVDSDADKGMGWTPPATIRPSMTVE
                     TSPGNFQFWFFLRAAINAELAQKLGERIRRAVNSDHDTGNPTQPYRIAGTINYPNAKK
                     TARGRDTVWTRLIALDPNALWTPEEIERAFPLSEQPRTNGGAPTPGGSASEADIPADT
                     MRVIRDGPTKGNARDRSLAFFNVMIALKRLGFTVEGILELLERHPEGVAKKYEGRLRQ
                     EVERAYNKITTDDEPSGAQTVIAPVVSPTIIPTTSQPLADAHATFKKWLGQDYDTDVL
                     DLTLAASAAERLTGDPLWLLVISGSGNAKTETVQSLAGAGALVTSTITSEGALLSATP
                     RQLQSGRATGGLLLKLGNRGVLVIKDFTSVLSMDHKARGLVLAALREIYDGKWERNVG
                     TAGGRTLPWNGRIAIVGACTTAWDTAHSVIAIMGDRFVIVRADSETDHVRTRSAWQAI
                     RNTGKEATMRAELAAAVGGLIASASTEEYQLTPAETDRLIKLANIVTRTRTGVERDYK
                     GDTVDKHALEMPTRFVKQLTQVVRGTVAIGMPPETAMRLASRCARDSIAPLRRDILLD
                     VAAHPQARPREVARRIVRPRTTVERELRALHLLNALDCDQVDMVQGGRQHTFERYSLA
                     PILDRQLLLSM"
     gene            complement(3815..4030)
                     /locus_tag="VM910_07290"
     CDS             complement(3815..4030)
                     /locus_tag="VM910_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62381.1"
                     /translation="MIDPDMQLAYRAPGDTRRLLIYPLCGGDVLRQGKPNAHRYGLSL
                     EFSCQQCSSVVELAITPHDGQTLVYWR"
     gene            complement(4027..4530)
                     /locus_tag="VM910_07295"
     CDS             complement(4027..4530)
                     /locus_tag="VM910_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62382.1"
                     /translation="MALPSELTTRENEHHGSELGDVIRQLASDNQGVAFAALCAMARK
                     LEKYGCSFLDLADHIENGSGGLSEDDKKKIRSEIENARAIGYAEGVAAAESKQHGTGA
                     FRNTDGKLDWKEVALYVQREKHRLNPKHHDFINKVAAQTVYDREPTQPMHKYLHSLFY
                     QLGGKIT"
     gene            complement(4592..5248)
                     /locus_tag="VM910_07300"
     CDS             complement(4592..5248)
                     /locus_tag="VM910_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62383.1"
                     /translation="MPNFNRATLSLEPINEAVNAAIERAAATAAEMPRPYLGASIIGH
                     ECARRIQYDWWCKPVLAARTREIFDRGHYFEQRSRQRLITAGFKFAPPEALAFTAVNG
                     ALRGHADGIIIHGPDLPGAYVIYPFVWEHKAVNAKNWRAVERDGLEKTFRQYAAQVAL
                     YQAYLDVTNPALFTVTNADTCEWLHFLVPFNAERAQLWSDRAVNIIEAARWRVAAAGL
                     "
     gene            complement(5259..5867)
                     /locus_tag="VM910_07305"
     CDS             complement(5259..5867)
                     /locus_tag="VM910_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62384.1"
                     /translation="MTEPKDWYTPATFDNGESGYWMTREGVIFYAMKTDGRVAVQAQV
                     EIANIVTAWYRGEIFPRGAAPTVAMILEFDSKAEADTKDSSYPKAAPQRPEPVEILGS
                     VSGLQKLLVEMAEENRGLDHAALTELFDAHPLVMGVWQAEGELNRPFGHGFLTLKGAE
                     YLIGQVKQQRTKKIRAVMTAVWCNNREHAELLRQAFTGPEQP"
     gene            complement(5879..6445)
                     /locus_tag="VM910_07310"
     CDS             complement(5879..6445)
                     /locus_tag="VM910_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62385.1"
                     /translation="MKKKINKIGEVSLSALEDQWQRDATAAAIAAARGVVQMDGPVPP
                     GTPIGRLSDTEWGWILAAMLFAWIGKRAEQASAEQLDTERTIRMVALDPQPWDAGAVA
                     AILPELADACSDVVDWSKPLAQWPREMIIEFLLRAMPLIRKAAIARDLGDKGVSRKSN
                     AATIARQANAAAGGPLMTPDEFNDEIGI"
     gene            complement(6442..7038)
                     /locus_tag="VM910_07315"
     CDS             complement(6442..7038)
                     /locus_tag="VM910_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62386.1"
                     /translation="MPFNYSDAPPPRDFELIPHPTVATCILHINAGGVGEDGMLKRSK
                     DGTCEMLACEFTVIDGLYKGRKFWEYWVMVGTTDGHKQSVGWNQGMIKAIIDSALGLK
                     PDDMGPQAYAARNLSYKQLEGLTFIGKIGVEKGKPKNDNSGENWPDKNILLGVITPDK
                     KDWHPVEQPPPFNGGGSGAQAAAPSSAAPPIARPGWAS"
     gene            complement(7052..7897)
                     /locus_tag="VM910_07320"
     CDS             complement(7052..7897)
                     /locus_tag="VM910_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI62387.1"
                     /translation="MMKFIPADARLAEKSGPKILIVGSSGVGKTSLLRTLSAKMLASA
                     VFIDIEAGHLAVDDVPIASVRPRTWDECRDLACALGGPNPALPPTASYSEAHHNKVME
                     DHELARLADYPILFVDSLTTAGRLCFTWAEQQPEAFTDRGRKDLRSIYGLHARSMVGW
                     LNQLQHAREKTVVFVAVLERNIDELNIATWQPQIEGAKTGRELPAIVDEIITMQWIDF
                     GDRKPVRAFVCTNPNPWGYPAKDRSGRLEQLEPPNLGALIDKLTRPGQRKPFVTVSPE
                     QPTQT"
     gene            complement(7894..8160)
                     /locus_tag="VM910_07325"
     CDS             complement(7894..8160)
                     /locus_tag="VM910_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62388.1"
                     /translation="MKRPNNELVREAVKAVTAHGFVPIVRNGGKHVKLSWTSCGKLAR
                     FAYESRTGKTPPAFTEAHFEQANTGAVIETYSAIDLGDEQEAQS"
     gene            complement(8330..8605)
                     /locus_tag="VM910_07330"
     CDS             complement(8330..8605)
                     /locus_tag="VM910_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62389.1"
                     /translation="MYRGVSLLWQPTMVQCAGIWRINRTYVGWAVKRPCDRALVESGA
                     VPLALPTTKALPPPMSPEEKLVDVVSQVGINGTHDLLVRIERARAAA"
     gene            complement(8858..9673)
                     /locus_tag="VM910_07335"
     CDS             complement(8858..9673)
                     /locus_tag="VM910_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62390.1"
                     /translation="MQALYTADLENRNDNSGQEQHCVVTLDELAAQIREADRAVRAAE
                     SNALDSAYVAGERVLKVRGRVPNLRRWLEENGFAFSTMFLYLKLFLNREKIEAARKDS
                     PHLSLRAAVRLIGKKRIAGAKTSGRTDAGSGGPQDDVAKVLAVFDAVPAEAIKAALEK
                     RGFAWFLKRMPASWIPLLMARVAHLPRKSDETDEPFLRGSEVLRRALSLVMIASDPKT
                     TPAVAASNEKEAVAALRQLNFVLAGAHIDTVTIVRKHAKANRCVETKKSRRAA"
     gene            complement(9773..10039)
                     /locus_tag="VM910_07340"
     CDS             complement(9773..10039)
                     /locus_tag="VM910_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI62391.1"
                     /translation="MGKKRKAPAPAKAAASASALSIKALPVKALMEDTTVSVDDAALL
                     LGLSRGSAYAGVKEGQIPSLRIGNRIRVPTAPLRAMLKLEANAA"
     gene            10193..10717
                     /locus_tag="VM910_07345"
     CDS             10193..10717
                     /locus_tag="VM910_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62392.1"
                     /translation="MDAEQAEQEVAVKRRVGRPPGRKTPHRPTLTTSARVSAEIYERF
                     VKAAQASGRTLSAEVIQRACEGLEWEKTRQDAQAMLAKAGQDARAIVANAEFVTEKTS
                     KTELVKELQRRGYRYVRGVNGAAWFDPGVDSITWIADPALLDDLLDRAVARTLETIGA
                     RALAKPQTKPRRKS"
     gene            10714..11880
                     /locus_tag="VM910_07350"
     CDS             10714..11880
                     /locus_tag="VM910_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI62393.1"
                     /translation="MRGHIRKRGSNSYELKYDAGREGGRRRTRYKNVKGSRREAQTEL
                     NRLLAQVADGGHVDPHRLSVGAYLCERVAHWRAMNTISPKTEQGYNELLKNQILPHLG
                     TVPLQKLTARDIENWHATLLSRGRKGPSGRPGQGGVSPRTVAHAHRVLNKALREAVRH
                     ELVLRNVCTAQRAPKVVPVEMHILSLAQVKEFPTALDGHELAAPAVTALFCGLRRGEI
                     LALRWGNIDFMAKLLKVRESLEQTRAGLRFKQPKSRAGVRDVALPDIVIKVLHGQRQR
                     LLERRLLLGQGKLGDSDLVFPAWDGSPQGPEAFSAAWARFAQERGLGVTFHELRHTHA
                     SQLIDAGVPLPEIAHRLGHSSPAITLSTYAHLFKKDDSKAAKAINDALGGNHGV"
     gene            11870..12133
                     /locus_tag="VM910_07355"
     CDS             11870..12133
                     /locus_tag="VM910_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62394.1"
                     /translation="MEYKLDDALSELGFTLRGDGSFKSGCEIVFNLTQGEDEAGNPNE
                     EIFYRVEVCTSKGTLFFIVHEADFIVGKGGPLGGEADHGPTLA"
     gene            12114..13778
                     /locus_tag="VM910_07360"
     CDS             12114..13778
                     /locus_tag="VM910_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62395.1"
                     /translation="MALPSPNVCELIRAHFILLGSPSWKEEERDRLLKLLAEHGLKWG
                     DLPEVLLATANLGATTPPGLLLRHHFGWGDMPKVFADASAPQSPKKLQPFRRLAQCFG
                     LFGQENANAAANTRKQLDAFLARHGLTWADLTAILAASATPPPSDPRVGAPHPYSKPE
                     YTPAGLVEGLVGSYVTMSEPVSVISSLWTCFTHVYTRFAIAPRLAVVSEEPQSGKSTL
                     GKATKRLVYRPNRTVLSTGAAIQRFLGLGHGTVMTDQMDHLEAEARRALQRIWNLGYD
                     REASEISLVIGGKEAFFNLYAPMFSAGIRGFLAGMTTFLAPTQQSRSFALEMERFTEQ
                     TKPKYEYNVELSPENPGSEKLIRVFDQVYSFLCGWAANVKLNPKPSIPPELIARYGDN
                     ARGLVSIADSCNPEWGQRIRDALMFFFEKEKQERPQVTIVRHGIAIFDAFGLPQRSDV
                     IPTVEFDKQLKRLDLPDARWTRYRGPSGLEYAHPITIAERAALLRKAKPLVSKTCWPP
                     GPRTLGGSFNGYVRGQFEDALQGYEQEGRVKPERGHLRLITPTSDG"
     gene            complement(13844..14131)
                     /locus_tag="VM910_07365"
     CDS             complement(13844..14131)
                     /locus_tag="VM910_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62396.1"
                     /translation="MDHLRKRGLRFWPSIPQPFDPDLILLNMVTVADAADVVIVMGWT
                     HAVAYHRVALGSILSALPRCDGSGPSRRITPRVAQLGGNSGGKNFGAVWQL"
BASE COUNT         2597 a         4504 c         4502 g         2767 t
ORIGIN      
        1 gccacgctcg cggcagaact tgcagtacgg ctcgagccgg agttggtggc gctttcgctt
       61 ttgccaacgg gtcaggccgt accaattgcg ccagggaact ttggggtctg gagttccgcc
      121 gcgggtcatg ggtcactcag aactcaggga gtggagagac ctttggcctg gtccgtctca
      181 ccggcgcggc cagcgttccg gatggacgtt gctcgcgcac ctgtgggtcg agatcccctg
      241 tgctggctgc ggcgcctagt ccggctctcc tcactgcaac cgactgccag gttcccgctc
      301 ttgcttgcgg aatccgctcg ctcgagcatg actcgaaata gggcacccgc ggcacgccgc
      361 ccgcgcagtg cgtcgcgctc gcgtcgaact ttgtccaact cggctcgcag cgcggcgcac
      421 tgctgacacg aggggggccc ggtcctcatt catagttctt cgccttaata gccccgcatt
      481 gccggccggg gcgccaggac agaggtacct ctgagccgcc tggtgtcttt gctgccgtcc
      541 tcggcaaaca ccgaaagctg cgtccagagg ccaccagagc cttgggcccg gccggcgcgt
      601 cagggcgcca actagagtcg gcgcgccagc tacgtagacg tgatggttca tgggggtcgc
      661 cgcaactgac tgagcagctt gacggcgcca gcatcttgcc gagcagcgag cgcggccatt
      721 ccgccggtgg caaagaccgc caccgccttc aggagctcgg ccagctggcg cgcggatccg
      781 gagtcgaagc ggccataggc gcctcgagtg agctcagcga tgttccggaa tacgcgctcg
      841 acctgatggt tgtttccttc ctggaacatg aacgccggca ccctgagccg cccgagctcg
      901 ctggcttcgt gggcgagcgt atcgggattt tcctccatgg cgtcgccgac gaagactagg
      961 gcactgaccg gcagcagctt ggtctccttc cgggcgtgga tcaggacctt ctcgagctgg
     1021 gtatgcccgg cttcgcattt gatctgcgac atggccttgg tattaccggg gcagcctcgg
     1081 cgagtgcaaa gcctcgcgct ggttctcgca atccgagccg ctcaagacta gctgcatgtc
     1141 gagtccaccg gcagtagcga cctcccggaa catctcggcc tgcaattggc aggcgagatc
     1201 ccacgtctct tcccggctca tggtggcgtc gagggcaaag atgagacgac cgcgcgtgcc
     1261 tgcggcagcc gtggtgcgcc ccctaatatt agcgaggaag gcatcgagct cggagcgcgc
     1321 cggcgcgctc tcgccgcggt tgatgatcga ggatttgtcc tgctcgctgg tcatttaacg
     1381 tctcccgttg cttttgtttt tggcgcgacg gcgcactcaa accttactgg cccggaaaag
     1441 tgtgagagta gagattcaga gagattatta aggggaacat tctctactcc catttttccg
     1501 ggccagggcg aaaaccgcag ccacccacat ttccggtcca tgctaaccat cacatcgata
     1561 acaataattg acggtcgagt atcggtgcca ggctatagcg ctcgaatgta tgttgccgcc
     1621 caccctggac catatccacc tgatcgcagt ccagcgcgtt gagcaggtga agtgctctca
     1681 gttcacgctc caccgttgtg cgcggccgga cgatgcggcg agcaacctca cgcgggcgag
     1741 cctgggggtg ggcagcgaca tcgagcagga tgtctcgccg taacggtgcg atgctatcgc
     1801 gcgcacaccg gcttgccagc cgcatggccg tttcgggcgg catgccaatt gccaccgtac
     1861 cgcgcacgac ctgggtcagt tgcttgacga agcgggtcgg catttccagc gcatgcttat
     1921 caaccgtatc acccttgtaa tcgcgctcga cgccggtgcg cgtccgagtc acgatattcg
     1981 caagcttgat caggcgatca gtctcggcag gggtgagttg gtactcctcg gtgctggcac
     2041 tggcgatgag tccaccgacg gcagccgcca gctcggcgcg catggtggct tctttgccgg
     2101 tattgcggat ggcttgccat gccgaacggg tgcggacatg gtcagtttca gaatcggcgc
     2161 gaacgatgac aaagcgatcg cccatgatgg caatgacgct gtgcgcggtg tcccaggcag
     2221 tagtgcaggc gccgacgatg gcaatgcgac cgttccaggg gagcgttcgt ccgccggccg
     2281 ttccgacatt gcgctcccac ttgccgtcgt agatttcgcg caacgcggcc agaacgagac
     2341 cgcgcgcttt gtggtccatc gaaaggaccg aggtgaaatc cttgatgacc agcacaccgc
     2401 gattgccgag tttgagcaac aagccgccgg tggcccggcc actctgcaat tgacgcggag
     2461 ttgccgacag cagagcgccc tcggatgtaa tcgtgctggt gacgagcgcg ccggcgcccg
     2521 cgagcgactg cacggtttcg gtcttggcat tgccggaacc ggaaatcacc agtagccaaa
     2581 gcggatcacc ggtcaaccgc tcagctgcgc tagcagcgag agtgaggtcg agcacgtcgg
     2641 tgtcgtaatc ctgacccagc catttcttaa aggtcgcatg cgcgtcggct agcggctgcg
     2701 acgttgtcgg aataatcgta ggcgacacca ccggcgcgat cacagtttgc gccccgctcg
     2761 gctcgtcgtc cgtagtgatc ttgttatagg cacgctcgac ctcctgtcgc agccggcctt
     2821 cgtacttctt ggcgacgccc tcaggatggc gctcgagtaa ttcgagaatg ccctcgacgg
     2881 tgaagccgag acgcttgagc gcgatcatga cgttgaagaa tgcgagcgag cggtcgcgcg
     2941 cgttgccctt ggtggggccg tcgcggatga cgcgcatggt gtccgcagga atatcagcct
     3001 cgctggcgct gccgcctggc gtcggcgcac cgccgttggt cctcggctgc tctgacaatg
     3061 gaaatgcccg ttcgatctcc tccggtgtcc atagcgcgtt gggatcgagc gcgatcagcc
     3121 gggtccacac agtgtcgcgt ccgcgcgcgg tcttcttggc gttcgggtag ttgatcgttc
     3181 cggcgatgcg atagggctgg gtcgggttgc cggtgtcgtg gtcgctattg acagcgcggc
     3241 ggatgcgctc gcccagcttt tgtgcgagct cggcattgat tgctgccctg aggaagaacc
     3301 agaactgaaa attgccgggc gaggtctcca ccgtcatgct cgggcggatc gtcgccggtg
     3361 gcgtccagcc catgcctttg tcagcatcgc tgtcgacaac gagggcaaat accgcaacgg
     3421 tatccttgag cgtaccgcgc tcgctaccgc gcaagctcgc cggaaccaag cggccctcga
     3481 tgtagacgtt atggccggcc tcgcagtcgg caatcgcggc gctaatcatg cggtcgatgt
     3541 cgtcgagcgt gtaacggctc ggcaccaggg tttcggatac gggatgcagt cgtgagagct
     3601 gtagcaggcc tggcgttgcg cccgcggctg ccttcgcctg cgtcgcaatc gcctggatgg
     3661 actcgcgcac cgcgcgcgga tccggcgtca gtagaatttt ctgcgcctcc gctggagtgg
     3721 tcttttcgat ttcgtcgtcg atgaggccgc ggctgcgcaa ggcgtccttc atcgcattgg
     3781 taagcatgaa tgggatgccg gccatggcct cctcctagcg ccaatagacc agtgtctgtc
     3841 cgtcgtgggg agtaatcgcc aactcgacaa cagaactgca ttgttggcac gagaactcga
     3901 gcgagagccc gtagcgatgc gcattcggtt tgccttggcg aagaacatca ccgccacaca
     3961 gcgggtatat cagcaagcga cgagtatctc cgggggcgcg ataggcaagc tgcatatccg
     4021 gatcaatcat gtgatcttcc ctccgagctg gtagaacaga ctgtgcaggt acttgtgcat
     4081 cggctgggtt ggctcgcggt cgtacacggt ctgcgccgcc accttgttga tgaagtcgtg
     4141 atgtttggga ttgagccgat gcttctcacg ttgcacgtag agcgcgacct ccttccaatc
     4201 gagcttgccg tcggtgttac ggaacgcgcc ggtaccgtgt tgcttgcttt ccgccgccgc
     4261 gacgccctcg gcgtagccga tggcacgggc attctcgatc tcgctgcgga ttttcttctt
     4321 gtcgtcctcg ctcaggccac cgctgccgtt ctcgatgtga tcggcaaggt cgagaaaact
     4381 gcagccatac ttttctagtt tccgcgccat cgcgcacagg gcagcaaagg ctacaccctg
     4441 gttgtccgat gccagttgcc ggataacgtc gccaagttcg gagccgtggt gttcgttttc
     4501 ccgggtggtg agctcggacg gcaatgccat ggctcacctc cagcaccgct ctttgtgcgg
     4561 gcacatcttg cagcgccaat cttcgggatc atcaaagccc cgcggcagca actcgccagc
     4621 gcgccgcctc gatgatgttg acggcgcgat cggaccagag ctgcgcgcgc tcggcgttga
     4681 acggcacgag gaagtgcagc cactcacacg tgtcagcgtt ggtgacggtg aaaagcgcgg
     4741 gattggtgac gtcgagatac gcttgataga gcgcgacttg cgcagcgtat tgccgaaagg
     4801 ttttctcgag cccgtcgcgc tcgaccgcgc gccagttctt ggcgttgacc gccttgtgct
     4861 cccatacgaa cgggtaaatc acataggcgc cgggcaggtc cggaccatga atgatgatgc
     4921 cgtcagcgtg gccgcggagc gcaccattga cggcggtgaa ggcgagcgct tcaggcggcg
     4981 caaacttgaa accggcagtg atgagacgct ggcgcgagcg ctgctcgaaa tagtgcccgc
     5041 ggtcgaagat ttcgcgcgta cgggccgcga gtacgggttt acaccaccag tcgtattgga
     5101 tacggcgcgc gcactcgtgc ccgatgatcg acgcgcccag atagggacgc ggcatctccg
     5161 ccgccgttgc cgcggcgcgc tcgatggcgg cattgactgc ttcgttgatc ggctcgagcg
     5221 agagcgtggc gcgattgaag ttgggcatgg cacagtactc aaggctgttc gggcccggtg
     5281 aatgcttgtc gcagcagctc cgcgtgctca cggttgttgc accagacggc cgtcatcact
     5341 gccctgattt tctttgtgcg ctgctgcttg acctgtccga tcaggtactc ggcgcctttc
     5401 agggtaagga agccatgacc gaaggggcga tttagttcgc cctcggcctg ccacacgccc
     5461 atcacgagcg gatgcgcgtc gaagagttct gtgagcgcgg cgtgatcgag cccacggttc
     5521 tcctcagcca tctcgacgag aagcttctgc aacccggaca cgctgccgag aatctccacc
     5581 ggctcgggac gctgtggcgc cgcctttggg taggacgaat ccttcgtgtc ggcttcggcc
     5641 ttcgagtcga actcgaggat catcgccacc gttggcgccg cgccgcgcgg aaaaatctcg
     5701 ccgcgatacc aggcggtgac gatgttcgcg atctccactt gggcttgcac cgcaaccctg
     5761 ccgtcggtct tcatcgcgta gaagatgacg ccctcgcggg tcatccaata gccgctctcg
     5821 ccgttgtcga aggtcgcggg cgtgtaccag tccttgggtt cggtcatggc ctcgctcctc
     5881 aaattccaat ctcgtcgttg aactcgtcgg gcgtcatcag cgggccgcct gcagcggcat
     5941 tggcctggcg cgcaatcgtt gcagcgttcg atttacgaga gacgcccttg tcgcccaggt
     6001 cgcgcgcgat cgcggccttg cggatcagcg gcatggccct gagcaggaat tcgatgatca
     6061 tctcgcgcgg ccattgcgcg agcggcttcg accaatcgac gacgtccgag caggcgtcgg
     6121 ccaactcggg caagatcgcc gccaccgcgc cggcgtccca cggctgcgga tcgagcgcca
     6181 ccattcggat ggtgcgctcg gtgtcgagct gctccgcgct tgcctgctcg gcgcgcttgc
     6241 cgatccatgc gaacagcatc gctgcgagga tccagcccca ctcggtgtcg ctgagccgcc
     6301 cgatcggcgt accgggcgga acggggccgt ccatctggac gaccccgcgc gccgccgcga
     6361 tagcggcagc ggttgcgtcc cgctgccact ggtcttcgag cgcggagagg gagacctctc
     6421 cgatcttgtt gattttcttt ttcacgacgc ccaccccggc cgcgcgatgg ggggtgcagc
     6481 acttgatggc gccgcggcct gagctccgct gcctccacca ttgaagggcg gaggttgctc
     6541 gaccgggtgc cagtccttct tgtcgggcgt gatcacaccc aggaggatgt tcttgtccgg
     6601 ccagttctcg ccgctgttgt cgttcttcgg cttgcccttc tcgacgccaa tcttgccgat
     6661 gaaggtcagg ccctcgagct gcttgtagct gaggttgcga gcagcgtacg cctgcgggcc
     6721 catatcgtcc ggcttgagcc cgagcgcgga gtcgataatg gccttgatca tgccctggtt
     6781 ccaaccgacg gactgcttgt gcccatccgt cgtgccgacc atgacccagt actcccagaa
     6841 cttgcggccc ttgtagagcc cgtcgatgac ggtgaactcg caggcaagca tttcgcaggt
     6901 gccgtctttg ctgcgcttga gcatgccatc ctcaccgacg ccaccggcgt tgatgtgtag
     6961 gatgcaggtc gcgaccgtgg ggtgcgggat cagctcaaaa tcccgcggcg gcggcgcatc
     7021 gctgtagttg aagggcatgg tgtgcctcct ctcaagtttg cgtgggttgc tcgggtgaaa
     7081 cggtgacgaa cggtttacgc tgaccgggac gggtgagctt gtcgatcagc gcaccgagat
     7141 tcggcggctc caattgttcg agccggccag aacggtcctt ggcgggatag ccccacggat
     7201 tggggttcgt gcacacgaac gcgcgcactg gtttgcgatc gccgaagtcg atccactgca
     7261 tggtgatgat ctcgtcgacg atcgccggca gctcgcgccc ggttttcgcg ccttcgattt
     7321 gcggctgcca ggttgcgatg ttgagctcgt caatgttccg ctcgagcacc gccacgaaca
     7381 cgacggtctt ctcgcgcgcg tgctgtaact gattgagcca gcccaccatg ctgcgggcgt
     7441 gcaatccgta gattgaacgc agatccttgc ggccacgatc ggtgaaggct tccggttgct
     7501 gctccgccca ggtgaagcaa agccggccgg cggtggtcag gctgtcgaca aacaggatcg
     7561 gatagtctgc gagccgggca agctcgtggt cctccatgac cttgttgtgg tgcgcctcgc
     7621 tatagctggc ggtcggtggc aaggccggat tgggcccacc gagagcgcag gcgagatcac
     7681 ggcattcgtc ccatgtgcgc gggcgcacgc tcgcgatggg gacgtcatct acagcaagat
     7741 gcccagcttc gatatcgatg aacaccgccg atgcgagcat tttggcggat aaggtgcgca
     7801 gcagcgacgt cttaccgacg cccgaggagc cgacgattaa aattttaggg ccgctctttt
     7861 cggcgagtcg cgcgtctgcg gggatgaatt tcatcatgat tgcgcctctt gttcatcccc
     7921 gaggtcgatg gcgctgtagg tttcaatcac cgcgcccgta ttcgcctgct cgaagtgcgc
     7981 ctcggtaaag gccggcggcg tctttccggt gcgtgactca taggcgaagc gcgccagttt
     8041 cccgcagctg gtccaggaca gcttcacatg cttgccgcca ttgcggacga tgggcacgaa
     8101 gccatgcgcg gtcacggcct tgactgcctc gcgcacgagc tcgttgttgg ggcgcttcat
     8161 ttgaaactcc aagccttcgc cgcgacaggc gtgctatcgc ggtggctaat tccggttctc
     8221 ttgtctgctt cacggaaccg tcagcagcgg ctcggggaaa cagacccttg gagagggccg
     8281 cgaccgccac ggcattcgcg cgcgtggcga ccgcggtggt cgatgccaac taggcggcgg
     8341 cgcgtgcgcg ctcgatccta acgagtaggt cgtgcgtgcc gttgatgccg acctggctga
     8401 cgacgtcgac cagcttctcc tccgggctca tgggcggggg cagcgccttt gtcgtcggca
     8461 acgccagcgg caccgcgccg gactcgacga gcgcgcggtc gcacggccgc ttcacggccc
     8521 agcccacgta agtcctgttg atgcgccaga tcccggcgca ctgaaccatg gtgggctgcc
     8581 agagcaggga gacgcccctg tacatgtcag ctgcctcata ggccttctcg accgcgaaag
     8641 ctgggcgtgg ctcgcgtact ggccggtaac cagtcgcgag accgagttga acaactcagg
     8701 ggcaacgcta tatttaccgt tcccgttctt catgggaaac ctcataaggc cctggactgg
     8761 agggactgac ggtgaactcg tcagtccttc cctccgccgc cgccaggcct ggtggcgcgc
     8821 gacggtttcg ggcggaatac ccgaagctca aaatctctta ggcagcgcga cgagactttt
     8881 tcgtctcgac gcagcggttg gcctttgcgt gtttccggac gatggtgact gtgtcgatat
     8941 gcgcaccggc cagcacgaaa ttcagctggc gcaaggcggc gaccgcctcc ttctcgttgg
     9001 atgcggcgac cgcgggcgtg gtctttgggt cgctagcgat catgaccagt gacagggcgc
     9061 ggcggagcac ctcgcttccc cggagaaacg gctcgtcggt ttcgtcggat ttgcgtggca
     9121 gatgggcgac gcgcgccatc aacaggggta tccagctcgc aggcatgcgc ttcaggaacc
     9181 aggcgaagcc acgcttctcc agcgccgcct tgatcgcttc ggcgggcacg gcgtcgaaga
     9241 ccgcgagcac cttcgcgacg tcgtcctgcg ggccgccact gccggcgtcg gtcctgccag
     9301 aagtcttcgc accagcgatg cgcttcttcc cgatcaaccg cacggctgcg cgcaacgaga
     9361 ggtgggggct gtccttccgc gcggcctcaa tcttctcccg gtttaggaag agcttcaggt
     9421 atagaaacat ggtcgagaag gcgaagccgt tctcttccaa ccagcgcctc aagttaggga
     9481 cgcgacccct caccttgagc acgcgttcgc ccgcgacgta ggcggagtct aacgcgttag
     9541 actccgcggc ccggacagcc cgatcagcct cgcggatttg ggcggcgagc tcgtcgaggg
     9601 tgacgacgca atgctgctcc tgtccggaat tgtcgttgcg attttccaga tcggccgtat
     9661 atagggcttg catttgcgga ccttccgagg ctttgacggg tttcgggatt gacgcagaca
     9721 cgcagacaag tgcccccggc gcagcagccg ctgccccggg ggcttcattt atttaagcgg
     9781 cattcgcctc gagcttgagc attgcgcgta acggcgcggt cggcacgcga atgcgattcc
     9841 caatacgaag ggaggggatt tgtccttctt tgaccccggc gtaggcgcta ccgcgactca
     9901 acccgagcag cagggccgcg tcgtcgacgc ttacggtcgt gtcctccatc agcgccttca
     9961 ccggcagtgc cttgatagac aacgccgatg ccgacgccgc cgccttcgcc ggcgccggcg
    10021 ccttcctctt cttgcccata gcgggctccc tttctgtgtc tgatggccgg acattgtagg
    10081 cacccgtatc gggggtcaag aattgtttgc catacggttt gacacagtgg cgtaagcggt
    10141 gatacgggtt gccacgacac caacctgtct ctctggacgc gaggaggtcg ctatggatgc
    10201 cgagcaagcc gagcaagagg tcgccgtgaa gcgaagggtc ggacgccccc cggggcggaa
    10261 gacaccgcac cgaccgacac tcaccacttc cgctcgagtg tcggcagaga tttacgaaag
    10321 gttcgtcaag gcggcgcaag ccagcggtcg cacgctgagt gcggaagtaa tccagcgcgc
    10381 ttgcgaaggc cttgagtggg aaaagacgcg ccaggacgca caagcgatgt tggccaaggc
    10441 gggccaggat gcgcgggcga tcgtagccaa cgccgagttc gtcacagaaa aaacctcaaa
    10501 gacggagctg gtgaaggagc tccagcgccg cggctaccgc tacgtccggg gcgtaaacgg
    10561 cgccgcctgg ttcgatccgg gcgtcgactc catcacctgg atcgccgacc ccgcactgct
    10621 ggacgacctc ctggaccgtg ccgtcgcccg cacgctcgag accatcggcg cccgcgcgct
    10681 tgctaaaccc cagaccaagc cccggaggaa atcgtgagag gacacatccg caagcgcggc
    10741 agcaattctt atgagcttaa gtacgacgct ggccgggagg gcggtcggcg ccggactcgc
    10801 tacaagaacg tcaagggcag ccgccgcgag gcgcagaccg agctcaatcg cttgctggca
    10861 caggtcgccg acggcggcca cgtcgatccc cacaggctca gcgtcggggc gtacttgtgc
    10921 gagcgggtag cgcattggcg cgccatgaac acgatctcgc ccaagaccga gcagggctac
    10981 aacgagctgc tcaagaacca gatcttgccc cacctcggca ccgtgccgtt gcagaagctg
    11041 accgcgcgcg acatcgagaa ctggcacgcc acgctcttga gccgaggtcg caagggcccc
    11101 agcggtcgcc caggccaagg tggcgtttcc cctcgcaccg tcgcgcatgc gcatcgagtc
    11161 ctcaacaagg cgttacgtga agccgtccgg cacgagctcg tgctgcggaa cgtctgcacc
    11221 gcccagcgcg cgcccaaggt cgtgccggtc gagatgcaca tcctctccct cgcgcaggtc
    11281 aaagaattcc ccaccgcgct cgacggccac gagctcgcgg ctccggcggt gaccgcgttg
    11341 ttctgcggcc taaggcgcgg cgagatctta gcgctgcgct ggggcaacat cgacttcatg
    11401 gccaagctgc tcaaggtgcg cgagagcctg gagcaaacca gggccgggct acggttcaag
    11461 cagcccaaga gcagagccgg cgtccgcgat gtcgcgctcc ccgacatcgt tattaaagtc
    11521 ctgcatggac agcgccaacg cctactcgaa cgccggctcc tgctcggcca aggtaagctc
    11581 ggtgacagtg atctggtgtt cccagcctgg gacggctccc cgcaggggcc cgaggcgttc
    11641 agcgcggcct gggcccggtt cgcccaggag cgcgggctcg gagtgacgtt ccatgaactg
    11701 cgccacacgc acgcgtcgca gctgatcgac gccggcgttc ctctccccga gatcgcgcac
    11761 cggctcggac attccagtcc ggcgatcacg ctctccacct atgcacactt gttcaagaag
    11821 gacgacagca aggccgcgaa ggcgatcaac gacgcgctgg gaggaaacca tggagtataa
    11881 actcgatgac gcgctctccg agcttggctt taccctgcgc ggtgatggct cgttcaaatc
    11941 gggttgtgag atcgtattta acctgactca aggcgaagac gaggcgggaa atccaaacga
    12001 ggaaatcttc taccgggtcg aagtttgcac ttccaaggga acgctcttct tcatagtgca
    12061 cgaagccgat ttcatagtcg gaaagggtgg ccccctgggc ggagaagcag accatggccc
    12121 taccctcgcc taatgtttgt gaactcatac gcgcgcactt cattttgctt ggctcgccca
    12181 gttggaaaga ggaagagcgc gatcgcctgc tcaaactcct ggccgaacat ggtttgaaat
    12241 ggggcgacct gcccgaggta ttgctcgcca ccgcgaacct cggcgccacg acaccgcccg
    12301 gcctattgct gcgccatcac ttcggctggg gcgatatgcc aaaggtcttt gccgatgcga
    12361 gcgccccgca atcgccaaag aagctgcaac ctttccgacg gctggctcag tgctttggct
    12421 tgtttggtca ggaaaacgcc aacgcggccg cgaacacacg aaaacagctg gacgcgttcc
    12481 tggccaggca cgggctgaca tgggccgatt taacagcgat cctcgctgcc agcgctacgc
    12541 cgccgccgtc cgatccgcgt gttggtgcgc ctcatccgta cagcaaacca gagtataccc
    12601 cagccggttt ggtggagggc ctcgtcggat catacgtgac gatgagcgag cccgtctctg
    12661 tcatcagctc gctgtggact tgtttcacgc acgtctacac gcggttcgcc atagcaccgc
    12721 gactggcagt agtcagcgaa gagccgcaga gcggaaagtc cacactcgga aaggcgacca
    12781 agcgcctcgt ttaccgacca aacaggacgg tcctgagcac cggcgcggcg attcaaagat
    12841 ttcttggtct cggtcacggc accgtaatga ccgaccagat ggaccaccta gaggcggagg
    12901 ctaggcgagc actgcaaaga atttggaacc tcggatatga ccgcgaggcg tcggaaatct
    12961 cgttggtgat cggaggaaag gaggcgttct tcaaccttta cgctccaatg ttctctgccg
    13021 gcattcgcgg tttcttggcg ggcatgacaa ccttcctagc gcctacccaa caaagcagat
    13081 cgttcgcgct tgaaatggaa cggttcaccg aacagacgaa gccgaagtac gagtacaatg
    13141 tagagctaag tccggaaaat ccggggtcag aaaagctcat tcgggtgttc gatcaggtgt
    13201 attcgttcct ctgcggctgg gccgcgaatg tgaagctcaa tcccaagccg tcgatcccgc
    13261 cggaactgat tgcccgatac ggcgacaacg cacgcggcct ggtctcgatt gctgacagct
    13321 gcaacccgga atgggggcaa cgcatccgtg atgcgttgat gttctttttc gagaaagaaa
    13381 agcaggagcg tccgcaggtc accatcgttc gacacgggat tgcgatcttc gatgcgtttg
    13441 gcctgccgca aagatcggat gtgatcccga ctgtggaatt cgacaaacag ctaaagcggc
    13501 tggacctgcc cgacgcgcga tggacacgat atcgcggtcc tagcggtctc gaatacgcgc
    13561 atccaatcac gatagccgaa cgggccgcgc ttctgcgcaa agccaagccg ctcgtatcga
    13621 agacatgctg gccgccggga ccgcgaactc tcggaggaag cttcaatgga tacgtacgcg
    13681 gtcaattcga agatgctctg cagggatacg aacaagaagg ccgcgtcaaa cctgagcgcg
    13741 gtcacctgcg actgatcact cccacctccg atgggtgagg cgtgtgaccg gtgtgacggc
    13801 cattcccgcc ccacccccct tcccgatcgg gcggtcacac tcgtcacagc tgccacaccg
    13861 cgccgaaatt tttgccaccc gaattgccac ccaactgggc gacacggggt gttatccgac
    13921 gcgacggacc actgccgtcg caacgcggaa gtgccgataa aatcgaacca agcgcgacgc
    13981 gatgatacgc gacggcatgg gtccacccca tcacaatgac aacatcggcc gcatcggcaa
    14041 cagtaaccat atttaacagg atcaaatcag gatcaaacgg ctggggaatg gaaggccaga
    14101 aacgtaagcc gcgcttccgt aagtgatcca atcgcgcctc ctcaggtgct ggaacaatcc
    14161 ggcgacgtca tcggccggcc gtatccggct ttggttgcac ctggatgagc gcgcgaccta
    14221 gtatttcacc gcggaccccc atgcgcgccg gcctcgcttt gagcctcatc gccctcgtcc
    14281 tgccgctcgc gggcgcgcac gccgatccgc tcgccgattt ctaccgcggc cgcaacgtca
    14341 ccctcatcat cggctatagc gccggtggcg
//
LOCUS       DATLJT010000323         4479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2315821,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000323 DATLJT010000000
VERSION     DATLJT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315821"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VM910_07370"
     CDS             <1..306
                     /locus_tag="VM910_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI62397.1"
                     /translation="LGYMAHGGHGCISVTSNVAPRLCSEFHLAWQKGDVATALKLHDK
                     LMPLHTNLFIESNPAPIKYAMSLLGRMGETLRLPMVPVSEPTRAAVRGAMVHAGLIN"
     gene            359..775
                     /gene="mscL"
                     /locus_tag="VM910_07375"
     CDS             359..775
                     /gene="mscL"
                     /locus_tag="VM910_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVI62398.1"
                     /translation="MLKEFREFAMKGNVVDLAVGVIIGAAFGAIVNSLVSDVIMPVIG
                     AATGGLDFSNYFVPLSKSVTANNLADAKKQGAVLAYGNFITLSLNFLIIAFVLFIVIR
                     LMNQLKRLQEAAPPAPPKPSREEELLTEIRDLLKKA"
     gene            777..1250
                     /gene="smpB"
                     /locus_tag="VM910_07380"
     CDS             777..1250
                     /gene="smpB"
                     /locus_tag="VM910_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HVI62399.1"
                     /translation="MAEKSERAIKVVAENRKARFNYTIEDTIEAGLALTGTEVKSIRS
                     GKTTIAESYADSRDGEIWLINANIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVD
                     RDGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWSREKGRLMRARG"
     gene            1312..1887
                     /locus_tag="VM910_07385"
     CDS             1312..1887
                     /locus_tag="VM910_07385"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI62400.1"
                     /translation="MNQRNLLEVDWSKIPAPTDDGGAAHLKGMLMPPVGLLATDDTKV
                     TLSELAGRTVVFAYPRTGEPGKIALVDDWDMIPGARGCTPQTCAFRDLFADLKAAGAN
                     HVFGLSTQSNAYQAEMASRLHLPFPVLSDEKLEMTRALNLPTMEVAGLTLIKRLALII
                     DDARITHVFYPVFPPDRNAGDVLDWLKDNPA"
     gene            complement(1884..2552)
                     /locus_tag="VM910_07390"
     CDS             complement(1884..2552)
                     /locus_tag="VM910_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVI62401.1"
                     /translation="MTTTSTIRPLAAQRQTEPDHNCPLCPRLAEFRAATRAKEPDWFN
                     GPVPSFGDRDGRLLIVGLAPGLQGANRTGRPFTGDYAGDLLYATLRAYGFARGNYQAR
                     PDDGLQLIDCLISNAVHCVPPQNKPLPAEITTCRPYLAATIETMPRLAAIVALGRVAH
                     DTVLKILKLRSAAAPFAHGAVHQAGRLRLYDSYHCSRYNTNTGVLTPEMFRAVFARVK
                     ADLD"
     gene            complement(2530..3165)
                     /locus_tag="VM910_07395"
     CDS             complement(2530..3165)
                     /locus_tag="VM910_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HVI62402.1"
                     /translation="MSSTSNKIALFIDGANLYATAKTLGFDIDYKRLLKEFQGRGTLL
                     RAFYYTAIIEDQEYSSIRPLIDWLDYNGYTVVTKATKEFIDASGRRKVKGNMDIELAV
                     DAMELAEHIDQMVLFSGDGDFRSLVEAMQRRGVRVTVISTISSQPPMIADELRRQADV
                     FTDLVELQAKIGRDPSERPAPREREPRHHAPQFLQRATTMAPRGDDDDFDD"
     gene            3505..3897
                     /gene="rpoZ"
                     /locus_tag="VM910_07400"
     CDS             3505..3897
                     /gene="rpoZ"
                     /locus_tag="VM910_07400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVI62403.1"
                     /translation="MARVTVEDCIDKVDNRFDLVLLAAHRARMISSGSQLTVDRDNDK
                     NPVVSLREIAESTISPEDLKEELVHSLQKFVEVDEPEPDTVPLIGSAGASVDADDTEV
                     AVERMTEEELLKGLEGLAPPEEQPEEDE"
     gene            4116..>4479
                     /locus_tag="VM910_07405"
     CDS             4116..>4479
                     /locus_tag="VM910_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVI62404.1"
                     /translation="MAYRRHSPPQMQAAAEPAAVASAAPIKTAKAPRPRMMRQYDLVE
                     RVRSYNPDTNEDLLNRAYVYAMKAHGSQTRASGDPYFSHPLEVAAILTDLKLDDATIV
                     AALLHDTIEDTEATRAEID"
BASE COUNT          916 a         1355 c         1359 g          849 t
ORIGIN      
        1 ctcggctata tggcgcatgg cggccatggt tgcatctcgg tgacttcaaa tgtcgcgcca
       61 cggctgtgtt cggaatttca cttggcctgg cagaagggcg acgtcgcgac ggcactcaaa
      121 ctgcatgaca aactgatgcc gctgcatacc aatctcttta tcgaatccaa ccccgcgccg
      181 atcaaatatg cgatgtcgct gctcggaagg atgggcgaga cgctgcggct gccgatggtg
      241 ccggtgtccg agcccacccg tgcggccgtc cgcggcgcga tggtgcacgc ggggctgatc
      301 aactgagccc gattgcgcct ccagactctt cagccaagga cgaagaaggg gatcgagcat
      361 gctgaaggaa ttccgcgaat ttgcgatgaa aggcaatgtc gtcgacctcg cggtcggcgt
      421 catcatcggc gcggccttcg gtgccatcgt caattcgctg gtcagcgacg tgatcatgcc
      481 ggtcatcggc gcggccaccg gcggcctcga cttctcgaac tacttcgtgc cgctgtcgaa
      541 atcggtcacc gccaacaacc tggctgatgc gaaaaaacaa ggtgccgtgc tggcttacgg
      601 gaatttcatc actctgagcc tgaattttct catcatcgcc ttcgtgctgt tcatcgtcat
      661 tcgtctgatg aatcagttga agcgacttca ggaagctgcg cctccggcgc cgccgaagcc
      721 gtcacgcgaa gaggagcttc tgacggaaat ccgcgatctt ctaaagaaag cttgatgtgg
      781 cggaaaaaag tgaacgcgcg atcaaggtgg tcgctgaaaa tcgaaaagcc aggttcaact
      841 acacgatcga ggacacgata gaggccgggc ttgcgctgac cggtacggag gtcaagtcga
      901 tccgcagcgg caaaaccacg atcgcggaat cctatgccga ttcccgggat ggcgaaatct
      961 ggctgatcaa cgccaatatt ccggaatatc tgcaggccaa ccgcttcaat cacgagccga
     1021 agcgcccgcg aaagctcttg ttgcatcgaa agcagatcaa caaattgatg ggcgcagttg
     1081 accgcgacgg gatgacgctg atcccgctca agctctattt caacgagcgc ggccgcgcca
     1141 agctcctgct cgccgtcgcc aagggcaaga agctgcacga caagcgcgaa agcgagaaga
     1201 agcgcgattg gagcagggag aagggccgtc tgatgcgggc gcgtggatga ggtggtggcc
     1261 tcgagccggg catggctcct tccgaactgg aggcctttga ggagcaagca aatgaaccag
     1321 agaaacctgc tcgaagtaga ctggagcaag atcccggcgc cgaccgacga tggcggcgcg
     1381 gcgcatctga agggcatgct gatgccgccg gttggcttgt tggcgaccga cgacacgaag
     1441 gtgacgctgt ccgaattggc cggccgcacg gtcgtgtttg cctatccgcg caccggcgaa
     1501 cccggcaaga tcgcacttgt cgacgattgg gatatgatcc ccggcgcgcg cggctgcacg
     1561 ccccagacat gcgccttccg cgatctcttc gccgacctga aggccgcggg tgcgaaccac
     1621 gtgttcggcc tgtcgacaca gagcaacgcc taccaggccg agatggcatc caggctgcac
     1681 ctgccgttcc cggtgctgtc ggacgagaag ctcgaaatga cccgcgcgct caatttgccg
     1741 accatggagg tcgcaggact gaccttgatc aagcggcttg cgttgatcat cgatgatgcg
     1801 cgcatcacac acgtcttcta cccggtgttc ccacccgacc ggaatgccgg cgacgtgctg
     1861 gattggctga aggacaatcc ggcctaatcc aggtccgctt tcacgcgcgc aaataccgcg
     1921 cgaaacatct cgggcgtcag cacgcccgtg ttggtgttgt agcgcgagca atgatagctg
     1981 tcgtacagcc tcaatcggcc ggcctgatgc acagcgccgt gcgcaaatgg tgccgcggca
     2041 ctgcgcagct tcaagatctt cagaactgtg tcatgagcta cccgcccgag cgcgacgatg
     2101 gcggcgaggc gcggcatcgt ttcgatggta gccgcgagat atggacggca tgtcgtgatt
     2161 tccgccggca gcggcttgtt ctgcggcggc acgcagtgca ccgcattgct gatcaggcaa
     2221 tcgatgagct gcaggccgtc gtcgggccgc gcctggtaat tgcctctggc aaatccgtac
     2281 gcgcgcagcg tcgcatagag cagatcgccg gcatagtcgc cggtgaaggg gcgcccggtc
     2341 cggttggcac cctgcaaccc cggcgcaagc ccgacgatca gaagcctgcc gtctcgatca
     2401 ccgaacgagg ggacaggccc attgaaccag tccggctcct tggcacgcgt ggccgcgcga
     2461 aactcggcga gccgcggaca aagcggacaa ttatgatcgg gttcggtttg acgctgcgcg
     2521 gcgaggggcc taatcgtcga agtcgtcgtc atcgcccctt ggtgccatcg tggtcgcgcg
     2581 ctgcaggaat tgcggcgcat gatgacgtgg ttcacgctcg cgcggcgctg gacgctcgga
     2641 cggatcgcgg ccgatcttgg cctgcagctc caccaggtcg gtgaatacgt cggcctgacg
     2701 ccgcaactcg tcggcgatca tcggaggctg gctggagatg gtcgagatca ccgtgacgcg
     2761 gacgccgcgg cgctgcatgg cttccaccag cgaacggaag tcgccgtcac cggagaacag
     2821 caccatctgg tcgatgtgtt cggcgagctc catcgcgtcg accgcgagct cgatgtccat
     2881 attgcctttc accttgcgcc ggccacttgc gtcgatgaat tctttcgtgg ccttcgtaac
     2941 gaccgtgtag ccgttgtagt cgagccaatc gatcaacgga cgaatcgacg aatactcctg
     3001 atcttcgatg atcgccgtgt agtagaaggc gcgcaaaagt gttccgcgac cctggaactc
     3061 cttgagcagg cgcttgtagt cgatatcgaa accgagcgtt ttggctgtgg cgtaaaggtt
     3121 ggcaccgtcg ataaacagcg caatcttgtt ggaggtggat gacattcagt gactcgcgtt
     3181 gttgctatcc gtttttttgg cgcggcggca gtaccgcgca ttttcgacgt gccgccgatg
     3241 ccctgcggat tttcccacca cagacactac cgcaaattcg gtgagttctg ggcaataaac
     3301 gtatggtttt tacgacgcaa tgaagcgttt tcttgtccaa agaccgcaac cggcggattt
     3361 cagacgctcc aaactgtccg cggccgggat tagcaaagcg gatttgtgac gccaaatcac
     3421 aaattcgtct tgcgaaatgg ctcttgcccc tataactagc gcgcacaatc aacatatcaa
     3481 ggccaaattg cggagcgaca gccgatggca cgcgtcaccg tcgaagattg cattgataag
     3541 gtcgacaacc ggttcgacct cgttctcctg gcagcacatc gcgcccgtat gatctcttcg
     3601 ggatcacaac ttacggttga ccgggacaac gacaagaacc ccgtcgtctc ccttcgggag
     3661 atcgccgaat cgacgatttc gcctgaggat ttgaaggagg agctggtgca ctccctccag
     3721 aagttcgtgg aggttgacga gcccgagccc gacacggtgc ccctgatcgg atcggctggc
     3781 gcgagcgtcg atgccgacga taccgaggtc gcggtggagc gcatgaccga agaggaactg
     3841 ctcaagggcc ttgaaggctt ggcgccgccc gaggagcaac ccgaagaaga cgaataaagc
     3901 ttgttccagt tccgtttacc aaaggcccgg acaaacgtcc gggcctttgc ttttgcgacc
     3961 gtttttatgg ttaccatagg gtgcgagcag ctcgcggagc gctccgtcca gcgtccggct
     4021 gtctccgaat tgatcggagc agttttcgat tggcgctaga cttgctccga ggcagcatgt
     4081 cgccctccaa cgcccccggc aaaggcagtg attcgatggc gtatcggcgc cacagcccac
     4141 cccagatgca ggccgcggcc gaaccggccg cggtggcttc tgctgcgccg atcaaaacgg
     4201 ccaaggcgcc gcgcccccgc atgatgcggc agtacgacct ggtcgaacgc gtccgctcct
     4261 acaatcctga taccaacgag gacctcctca accgcgccta tgtctacgcc atgaaggcgc
     4321 acggatcgca gacccgggcc tccggcgacc cctatttttc acacccgctc gaggtggcgg
     4381 cgatcctcac cgacctcaag cttgacgacg ccaccatcgt ggctgcgctg ttgcacgaca
     4441 ccattgagga caccgaggcg acgcgggccg agatcgacc
//
LOCUS       DATLJT010000324         1651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3694166,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000324 DATLJT010000000
VERSION     DATLJT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694166"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VM910_07410"
     CDS             complement(<1..441)
                     /locus_tag="VM910_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI62405.1"
                     /translation="MMKTELLLETTGEFAAPVSTLCDMFDRAVANTPRTVALRHLGVA
                     LTYQEMGRAVAALARRLSLTLVPGEVIALVLPNSIEFQIAYFAALKALTAPALLNPLY
                     PAAQLAPLLRDAAPRAVLCAPATRDIVTRLAYELGIPNVVCLGRD"
     gene            complement(438..1337)
                     /locus_tag="VM910_07415"
     CDS             complement(438..1337)
                     /locus_tag="VM910_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVI62406.1"
                     /translation="MRTERLTTYELRDGVAWLGLNRPDKRNAINKGLLAELELGVRRA
                     QDDAQALVIFGHGPCFSAGLDLTEYRTRSAEEVYHGSRAWHAAFSLLLHGRIPAIAAL
                     HGATIGGGLELAAACQIRIADDTAFFALPEGARGIYVGGGASVHVARLLGTSRMADMM
                     LTGRVLDAAAAERTGLVQYVVPRGEAKPKAAELAAKVSAMAPLTILGVLHALPRIQDM
                     SEADGLFVESLMIAFTHTGPEAAARLSEFTAKRAPKVTPPSLASTVSSLPADGFGTAA
                     AAPNGAAVDSCLAGQEIYAEDNR"
     gene            complement(1440..>1651)
                     /locus_tag="VM910_07420"
     CDS             complement(1440..>1651)
                     /locus_tag="VM910_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVI62407.1"
                     /translation="GIENLRIADGSIMPRVVTGNTMAPCIVIGELASETLRSTHNLSQ
                     SEAVSTPRARRLPASSLSPTDDGST"
BASE COUNT          329 a          509 c          518 g          295 t
ORIGIN      
        1 atcccgcccc aagcagacca cgttgggaat gcctagttcg taagcaagac gggttacgat
       61 gtcccgcgtg gccggtgcgc acagcacagc ccgcggcgcc gcgtcccgca gcagtggcgc
      121 gagctgagcc gccgggtaga gtgggttcaa aagggctggt gctgtgagcg ccttcaacgc
      181 ggcaaaatag gcgatctgga attcaatgga attgggtaac acgagcgcga tcacctcgcc
      241 cggtaccaac gtcaaggaca atcggcgggc taacgcggcg acggcacgcc ccatctcctg
      301 atacgtgagc gcgacgccca gatgccgaag cgccacggtg cgaggtgtat tggcgaccgc
      361 gcggtcaaac atatcgcaga gggtcgacac gggagccgcg aattcgccag tagtctccaa
      421 caacagctca gtcttcatca tcggttatcc tcggcataaa tttcctggcc agcgagacat
      481 gagtcgacgg cagccccgtt gggggcggcc gcggccgtcc caaagccgtc cgccggcaaa
      541 ctggagactg ttgaagcaag cgagggtggt gtcaccttgg gggcacgctt cgccgtgaac
      601 tcgctgagtc gcgcagcggc ctcggggcca gtgtgtgtga atgcgatcat caggctctcg
      661 acgaagagcc cgtcggcctc gctcatgtct tggattcgcg gcagggcatg tagcactccg
      721 agaatggtga gcggcgccat tgccgacacc tttgcggcca gctccgcagc cttgggcttt
      781 gcttcacccc gcggaaccac atattgcacc aggccagtcc gctcggctgc cgcagcgtcc
      841 aagacccgcc cggtaagcat catgtctgcc attcgcgatg tgccgagcag ccgcgctaca
      901 tgcactgagg ctccgccgcc cacgtagatg ccgcgcgcac cttccggtag agcgaagaat
      961 gcggtgtcgt cagcaatgcg aatctggcat gccgctgcca gttcaagccc gcctccaatc
     1021 gtcgctccgt ggagagccgc gatcgcagga atgcgaccgt ggaggagtaa tgagaatgcg
     1081 gcgtgccatg cgcgggagcc gtgatagacc tcctccgcac ttcgcgtgcg atattccgta
     1141 aggtcgaggc cagctgagaa gcacggaccg tggccgaaaa tcaccagcgc ctgagcgtcg
     1201 tcttgagccc ggcgcactcc gagctcaagc tcggccagaa gccctttgtt tatggcattg
     1261 cgtttgtccg gccggttcag cccgagccaa gcgacgccat cccgtaactc gtaggtagtc
     1321 aatcgttcag ttcgcatctt ggttctcctc caccagggat tgcgcagaac catccgaaac
     1381 tcgatcatcg gatttcggag cagatatgca ggagcgcagt cgcggataaa aagaaattgt
     1441 cacgtcgaac cgtcatcggt cggagagagg gacgaggccg ggagccgtcg cgcgcgaggc
     1501 gttgacaccg cctcgctttg gctcaagttg tgcgtggatc gcagggtctc ggaagcaagc
     1561 tcgccgatga cgatgcaagg agccatcgtg tttccggtga cgacccttgg catgatggac
     1621 ccatcggcga tccgcagatt ctcgatcccg t
//
LOCUS       DATLJT010000325         5075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1626325,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000325 DATLJT010000000
VERSION     DATLJT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626325"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1415..2317)
                     /locus_tag="VM910_07425"
     CDS             complement(1415..2317)
                     /locus_tag="VM910_07425"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVI62408.1"
                     /translation="MANDEHVAMLKKGVDAWNAWRDENPDIRRPDLSEADLRRKNLSP
                     ANLSGAKLIGADLFGADLGEAKLIGADLNEADLTDAMLGKTVFGNVTLTRVKGLDHCK
                     HWGPSALDFQTLQKSGPLPAVFLRGVGLPDRLIEYLPSLLNEAIQHYSCFISYSSKDQ
                     AFAERLHTDLQNKGVRCWFAPHDMPIGAKIIDAIDEAIRLREKVLLILSEGAIASDWV
                     EGEVTRTLEEERTRKQVVLFPVRIDDAVMQTSEGWARLLRGQRNIGDFTRWKKHDSYQ
                     KSFERLMRDLRVNPQDGSDAPEAR"
     gene            2915..3139
                     /locus_tag="VM910_07430"
     CDS             2915..3139
                     /locus_tag="VM910_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62409.1"
                     /translation="MQVIQEGGDDRVSGTDADRHDDISHNGSSWFGGNTDSTETADLA
                     RVLINAADCSAVFYWASDISCPKNVLSMSN"
     gene            3494..4279
                     /locus_tag="VM910_07435"
     CDS             3494..4279
                     /locus_tag="VM910_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62410.1"
                     /translation="MLLATALLAPLPALADTVTIGYFDPAANMPGIQIIGTTSGPITT
                     TSPTVQLLGGPPFSPNAIMLGSGFGFDQIIATVIPPTGNIYSGGLGGPAPTFEFAFND
                     GFVPIQGGTIYLYATWSGAITAGNPITLPTMWGTSEMPAVPNGYTVTSQVLICNTPNP
                     FCGPFVGGINSFAGQDVFSDFVRTDTNTLTGVTATEPFKITEVFAFSGNPGPPQGDVG
                     AFIMTTPVGPTPVPGPIVGAGLPGLIAVAGGLLGWWRRRKKIA"
     gene            complement(4371..4538)
                     /locus_tag="VM910_07440"
     CDS             complement(4371..4538)
                     /locus_tag="VM910_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62411.1"
                     /translation="MAGVYSGRILKGEKPSDLPVQQPTKFELVVNVKAAKAIGLTIPE
                     PFLARADEVIE"
     gene            complement(4642..>5075)
                     /locus_tag="VM910_07445"
     CDS             complement(4642..>5075)
                     /locus_tag="VM910_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62412.1"
                     /translation="RKRRGLGKPETFNFLGFTFICGKTHAGKFQVKRKSRRDRMLAKL
                     RMVKEEMRGRMHQPIAVQGKWLWYVVRGYFNYHAVPTNSRALVAFRTEIAKSWHRVLI
                     RRGELTSLNWTRMNRLIDEWLPKPRILHPWPDKRFAVTHPR"
BASE COUNT         1039 a         1494 c         1491 g         1051 t
ORIGIN      
        1 cgaggcggac gtcgacggtg tcgggcatga gcgcagcttc aatctgcttg cgcaggccag
       61 ccaagctcag cgccgcagtt ggcggaacat tggtggtggt ggtgatgtag tagccgcgcc
      121 tcaggtcgta ggagacgccg acttcaatcc gttcgctggc catggctctc accgttcaaa
      181 tcgccttccg cacgagggaa gtcgagcagg tcattctcgt ttcctgcagg ccgagccgat
      241 cggctttaga gtgctaacgt cgcgcatgtt aggttttgct cccacttcaa gcgccggaag
      301 aacacaataa aatcatccct ccctgccgtg cctttggcaa gcgcgagccc cggcagtgcc
      361 aagacgttcg actcgcgctt tccatggccg gccgcggtta tattctggaa agcgggcgcc
      421 tccctttgaa aggtcgcaca gatttccggg atccagccga atttcggcca cagagactat
      481 tcgcgttcga gctgcgacgg tggaaggcgc agcttgagaa ctcgatcaac cggcaccgat
      541 atacgccctg agaccttcgt ggtgacgttc gctatcaaga cctgacggaa tcgagcagat
      601 tggacttaca cattgatacc gtccttgaat tttttgcaga aataatcgaa tgccactgcg
      661 ttcctttccg tgcgagtcat tggcacgtca actcgcgaaa cgttcggcac gactagatga
      721 atagcggcgg tgtcgccgaa ctcgcgggac gagtccgctg gggggcgcgt gggttcgatc
      781 catatgtagg gcttcgtttc gtccactgtt ctcatccttg ctggcccatg gttcaccgaa
      841 aagcgcccgt cgatggctcg cccgcatcta tctgcgcggt tagacacgcc atcgatcaag
      901 tgaagggggc gggcacggcg cccgtacgac cgaggctata catgccgaag gaaggcgtcg
      961 cgatcggcgc gactaagcgg acgggcggcg gccatgtcga tgatgacgcg gggcaacgcc
     1021 ttcatgccac gtgctttgat tggtaaaagg gcgctaggtt ggcgattata tacgcaacga
     1081 ttttggcctc ggtcattggg acgccccaat cggccaaaac tgcccataga accgaataga
     1141 gaagattgcc tgtaaaccat acaaccgcgc cgccgagcag gacgatgagc gtccagaata
     1201 ccgaactgaa tcgcggtttc ttaaagggca ttgctcaaac tcttcctctg agattgtatc
     1261 cccaggagga gtcaagtccc ggataatcgc cgcccgcagc gggaccttgg cacgcctgcg
     1321 ctcgcgctag gccatctggc gagcggtggc cttcgaccgg ctggctgtgg agcgtgtcaa
     1381 aggggcgcgg cacgcgcagc acgtcacaca cggctcacct cgcctcggga gcgtcgctgc
     1441 cgtcttgggg gttcacgcgc agatcgcgca tcagccgctc gaagctcttc tggtagctat
     1501 cgtgcttttt ccaacgggta aagtctccga tgttgcgctg accgcgcagc agacgagccc
     1561 aaccttccga ggtttgcatc acggcgtcat ctatgcgcac cggaaacaga acgacctgct
     1621 tacgggtgcg ctcctcctcg agcgtgcgtg tgacctcgcc ttcgacccag tcactggcga
     1681 tggcaccctc agacaatatg agaagaacct tctcccggag gcgaatggct tcatcgatgg
     1741 catcgatgat cttcgcaccg atcggcatat cgtgaggcgc gaaccaacag cgcactcctt
     1801 tgttctgcag gtccgtgtgt aggcgctcgg caaaagcttg gtctttgctg gagtagctga
     1861 tgaagcagga gtagtgctgg atcgcctcgt tcagcagcga gggcaaatac tcaatcaagc
     1921 ggtcgggaag ccctacgcca cgcaggaaca cggcaggtag tggaccagac ttctgcagag
     1981 tctgaaagtc gagggcgctt ggtccccaat gtttgcaatg atcaaggccc ttcactctag
     2041 taagcgtgac gtttccgaat acggttttgc ctagcattgc gtctgtcagg tccgcctcgt
     2101 tgaggtccgc cccgatgagc ttcgcctcgc cgaggtccgc cccgaagagg tccgccccga
     2161 tgagcttcgc cccgctgagg ttcgccgggc tgaggttctt cctcctgagg tccgcctcgc
     2221 tgaggtccgg acgacggatg tcggggttct catcgcgcca tgcgttccag gcatccacgc
     2281 ccttcttgag catcgcgacg tgctcgtcgt tcgccatgga agcaccctca gcgccaccac
     2341 attattctgg gacgggttga cgatgcgatc aatcaggttg gccagtttgc acacctacgc
     2401 acggctacga ggcgacgttc gaggccgcga tggcggcgtt cgcgaagagt tggcggcggg
     2461 agcaatgaaa agaaagcccc ggaaactgct cccggggctt agagctagat gaggaacgtc
     2521 tagtctccta gacgctccga gaccatccca aggcaccaat cgcgcaaggc gccgatcgcg
     2581 taagcgcccc gacccgccac ggccacgcga cgggtcagaa cttgccacgg cgcaacgggt
     2641 gtgaagcgtc atgaccaaga caatcctagt cgagaccgtg acgcgcagat cgggcgtcca
     2701 aaaaataatg ggctattcga agagtaacct gtgacagcag tcacagcagc tggggcagaa
     2761 agcgggcatg ctgctcgcgc ggtagtactg aaggaccccc cgaagtatct gccgaagcgg
     2821 gcaggcccta gcctccacct ccacgacaca tacgggcctg cccgcgccat agctgcgctc
     2881 cgccgagggc tgcgatgcac agagagacaa agacgtgcaa gtcattcagg aagggggaga
     2941 tgatcgagtt tctggcacag atgccgaccg acacgatgat atttctcaca atggcagttc
     3001 ttggtttggt ggcaatactg atagtactga gacggctgat ttagcccgtg tactcattaa
     3061 cgccgcagat tgcagcgccg ttttttattg ggccagcgac atcagttgtc caaaaaacgt
     3121 actctctatg agtaattgac tgagatattc cgcaaaagtt tcgtccaaaa aatttgattc
     3181 atgtccaaaa aatccaaatt gaccaaatta ctcagcttgg gcttgagttg attttgtggg
     3241 tgaccccgag tcaacggagc ccaattaaga agaaagaatg agagaacggt tcggagctgc
     3301 tctcgcggca atacttggca ccttggtgct acgccgtcat cagtaaaccg tctgatccac
     3361 cactcttaac acttgcaagg tgtgctggac gctttgtcag cacgacctgc ttgaatgagg
     3421 agtcatgaga aaactagctg ctctggtcgc gcagctgctt gcagcgagac tcattccgaa
     3481 acgatcattg ctattgctgt tggcgaccgc gctgctcgcg ccgctgccag cgctcgcgga
     3541 taccgtaact atcggttatt ttgatccggc cgcgaacatg ccgggtattc aaatcatcgg
     3601 caccacctcc ggtccgatca ctacaaccag cccgaccgtc caactcctgg gggggcctcc
     3661 attcagtccg aacgcgatca tgcttggatc ggggttcggg ttcgaccaga tcatcgccac
     3721 cgtgatcccg ccaaccggga acatctattc tggcggcctg ggcggcccag cgccgacgtt
     3781 tgagttcgcg tttaacgatg ggtttgtgcc catccaagga ggcacgatct acctgtatgc
     3841 gacatggtcg ggagcgatca cggccggcaa tccgatcacg ctgccgacga tgtggggcac
     3901 gtcagaaatg ccggcggtgc cgaacgggta cacggtgacc agtcaagtcc tgatttgcaa
     3961 cacgccgaat ccgttctgcg ggccgtttgt aggtggcatc aattcatttg ccggccagga
     4021 tgttttcagt gactttgtgc gaacggacac caatactttg acgggtgtta ccgcgacgga
     4081 acccttcaaa atcaccgagg tgttcgcatt ctcgggcaat cccggtccac cacagggtga
     4141 cgtcggcgcg tttatcatga cgacgccggt aggaccgacg cccgtacccg gcccgatcgt
     4201 cggtgctggc ctcccaggcc tcatagcggt agctggtggc cttctcggct ggtggcgacg
     4261 gcggaagaag atcgcctgag catccgacac aacgcacacg tcgtttgcgg cagccagcga
     4321 ttgccgatgt cgctgagcgt agcccgagtc tacaatcacg ctgcgccgct tcactcgatc
     4381 acctcgtcgg cgcgggcgag gaagggttcg ggaatcgtaa ggccgatcgc tttcgcggct
     4441 ttgacgttga cgacaagttc gaacttggtc ggctgctgaa ccggcaaatc actcggcttc
     4501 tcgcctttaa ggatgcgacc ggagtagacc cccgccacgc gaagggcgtc cgcaaggttt
     4561 gatccgtagt cgcggtaggg acgcgagtta tctcgcgccc cccgcacaga tccgtacgtg
     4621 cagctttccc gcatacggct cctaccttgg gtgtgtgacg gcaaaacgct tatcgggcca
     4681 cggatgaagg atacgcggtt ttgggagcca ctcgtcaatc agccggttca tgcgagtcca
     4741 gttgagactc gtcagctcgc cgcgtcggat gagcacccga tgccagcttt tggcgatctc
     4801 ggtccggaat gccacgagcg ctcgactgtt cgtcggcacc gcgtggtaat tgaagtagcc
     4861 gcgcacgaca taccaaagcc acttcccttg aaccgcgata ggctggtgca tgcgccctcg
     4921 catctcctct ttgaccatcc gtagcttcgc cagcatgcgg tcgcgccggc tcttccgttt
     4981 gacctggaat ttgccggcgt gggttttgcc gcagatgaag gtgaagccca ggaagttgaa
     5041 ggtctccggt ttgccgagcc cgcgccgctt gcggc
//
LOCUS       DATLJT010000326         6407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3235145,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000326 DATLJT010000000
VERSION     DATLJT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235145"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VM910_07450"
     CDS             <1..128
                     /locus_tag="VM910_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE transposase"
                     /protein_id="HVI62413.1"
                     /translation="VERFFAWIQWQRRILVRWEYYAQNFLGFVQLACLVVLFRRF"
     gene            308..517
                     /locus_tag="VM910_07455"
     CDS             308..517
                     /locus_tag="VM910_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62414.1"
                     /translation="MHDITSARLVVWLTDVHMRDTHPDGGANETHRRAGTIDWVRVQR
                     HAGENLSHEPLEFLAIVPKAGVAPK"
     gene            complement(573..710)
                     /locus_tag="VM910_07460"
     CDS             complement(573..710)
                     /locus_tag="VM910_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62415.1"
                     /translation="MVLEMRLPQRGMRGMEGGRPEKYDVVLFGPDGRRTYMSFIGEQS
                     V"
     gene            923..1324
                     /locus_tag="VM910_07465"
     CDS             923..1324
                     /locus_tag="VM910_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62416.1"
                     /translation="MTKPFRAKVAIVLAALYGLCILAPSAAFAFSANPGVAHCLTEGH
                     VGVHDHGGKAHVHADGTAHQHDNDAAAPPSGDNEKSRVPTCCSLFSVVAISDEPGLSL
                     GLYGPTSIVVPILADALSGRGPERINRPPIA"
     gene            1841..3178
                     /locus_tag="VM910_07470"
     CDS             1841..3178
                     /locus_tag="VM910_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVI62417.1"
                     /translation="MGVVATITQQELNKDAAAIRSPEEAKAASASIRRLLGRPLTADA
                     AVQVALLNNRGLQAAYAELAIADAERVGQSLPPNPTSSWRRIQAGPALESEMQLVTNI
                     IALATLPARSEIAAERFHQAQLHAAEETLRVAHEARSAYYRAVAARELIQFLTESQSA
                     AEAATKLTARLGETGAMNKLDQAREQVFYADLTAQLATARQREASEREVLIRAMGLWG
                     TSLDFKLPNALPPVPRRVQTLAGIEVDAVARRVDLQIARIELEVLAKSYGLTQASRFI
                     NILDAGYADKLEKRPDERFLMRGFDIRLQIPIFDFGEVRVRQAEATYMQAANRLLEMA
                     VNARSEARDAYRVYRSTYDIADHYQREVLPLRKVISEETLLRYNAMLIDVFSLLAEAR
                     QRIAATITAIEAKRDFWLATVDLKAAIAGGGRSSNKQERANPLAVSAGDAGAR"
     gene            3193..4566
                     /locus_tag="VM910_07475"
     CDS             3193..4566
                     /locus_tag="VM910_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HVI62418.1"
                     /translation="MISRRGFLGSAAVLASATAVSGRVQAASVPEAPSTKTTTMQPPI
                     FATGGPDYQPVVTLNGWTLPWRINGDWKEFHLVAEPVVREFAPGMKANLWGYNGQAPG
                     PTIEAVEGDKVRIFVTNKLPEHTTVHWHGMLLPNGMDGVGGVTQPHIKPGQTFVYEFE
                     LRKSGTFMYHPHSDEMVQMAMGMMGMFVVHPRDPKFRRVDRDFVFIMSSYLIDPGTYL
                     PKVAEMTDFNMWTWNSRVFPGIDPMPVRLGDRVRVRMGNLTMTNHPIHLHGHHFSVSC
                     TDGGWVPESAQWSESTVDVPVGTIRAFDMIADNPGDWAFHCHKTHHAMNAMGHDVKNF
                     IGVEKRDLARQVRKLVPDYMPMGSAGMADMADMEMPMPENTLPMMTGFGQFGPIEMGG
                     MFTIMKIREGLVSNDYRDPGPYKNLPGTVAYEVRGTAPNDPPARAPAAQPTPQKPSTR
                     PSSSHQH"
     gene            4583..5080
                     /locus_tag="VM910_07480"
     CDS             4583..5080
                     /locus_tag="VM910_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HVI62419.1"
                     /translation="MIICPYRLIAVALLLGFVDAGAVRTGLNGAAYAHEGHQSFAAGE
                     PGDPQKPARTIKVVMREDGKKMAFEPALVTVHKGEQIRFVLENAGIDDHEFVLATVKE
                     NRKHAELMKKFPDMEHADPNAKRLAPGAHGEILWKFTKPGTFEFACLIPGHREAGMLG
                     KVVVK"
     gene            5109..5417
                     /locus_tag="VM910_07485"
     CDS             5109..5417
                     /locus_tag="VM910_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HVI62420.1"
                     /translation="MTGRTLLAAVALAFSLDTSPHAARAQSDDFVNGQVTKVDESAGK
                     ITIKHGPLKKFDMNESMTMVFRAGDPAMLKAVKPGDRIRFVPDRINGQFTVKKIEKAK
                     "
     gene            complement(5471..>6407)
                     /locus_tag="VM910_07490"
     CDS             complement(5471..>6407)
                     /locus_tag="VM910_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVI62421.1"
                     /translation="VSSRILDKVYAVTDQAGTREVKTLYSGLYRPNGLAFANGTLYIA
                     ELAKISKVEKIEDNLDNPPKPVVIYDDLPKDEAHGWKFLTIGPDNKLYFNVGAPCNIC
                     MPSPAHAQLRRINLDGSGPEVVARGIRQVVGMDWHPALGQLYFTENSRDWLSEDIPED
                     KLNRLTQPGKDNFGYPYCHQGNIPDQEFGWGRSCDEFTNPVTLLGPHSAPLGMRFYTG
                     NMFPSEYRNAIFVARHGSWNRSKKFGGDIVVVSLNENGTVKSVDPFITGFIENNNYIG
                     RPADLLVLKDGSMLISDDYNGALYRVTYDSTTVGR"
BASE COUNT         1266 a         1984 c         1942 g         1215 t
ORIGIN      
        1 tcgtggagcg cttctttgct tggattcaat ggcagcgccg catcctcgtc cgttgggaat
       61 actacgccca gaacttcctc ggtttcgtac agctcgcttg cctcgttgtc ctcttcaggc
      121 gattttgaga taggttctag ccttcgcatc ggagccgagt tgggcgcaac acgcgagccc
      181 aggcgcgcat ccgtcgcagc cctcgtctgc ggcgccgcca gccggtcctg cggatgccaa
      241 agttgaattt gaaaacgaat ccatcatcgt tgtgcgcgtg cgcatggcgc cacatgagaa
      301 gacgccgatg cacgacatta cgagcgcaag gctggtggtg tggctgactg atgtgcatat
      361 gcgcgacacg caccccgacg gcggcgcgaa tgagacgcat cggcgtgctg gaacaatcga
      421 ctgggtaaga gttcaacgac atgcgggaga gaatctctcg cacgaaccgt tggaatttct
      481 ggcgatcgtg cccaaggcag gcgtggcgcc gaaatagctt gggcgcgcag ccaggcggcc
      541 agagttggtc ggctacgctg ctcgggaccc acttagacgc tctgttcgcc gatgaagctc
      601 atgtatgtcc gccgcccatc cggcccaaac agcaccacgt cgtatttttc cggtctgccg
      661 ccctccatcc cacgcatgcc gcgctgcggc aagcgcatct caagtaccac ttgttcacta
      721 tcgaggttct aacaccagcc caaattcagc gttacgcaga acttcgcggt tacaaggaag
      781 gcaaccgaca cccgcacggc cggcactagc aagttccagc cacatagtcg cccaagaagc
      841 gttgcaaaga ggggtcaatg ctctatcctt ggaggatagg ttcctgtgga ttgagtgctc
      901 acgacgcgag atgctatcgg tcttgactaa gccctttcgc gccaaagtgg cgatcgtcct
      961 cgccgcgctc tacggacttt gcattctcgc gccgtccgcc gccttcgctt tctccgccaa
     1021 ccccggcgtc gcgcactgct tgaccgaagg tcacgttggc gtccacgatc acggcggcaa
     1081 ggctcatgtc cacgcggacg gcaccgccca tcaacacgac aatgacgctg ccgccccgcc
     1141 gtccggggac aacgaaaaat cccgcgtgcc gacctgctgt agtttgttct cggtcgtggc
     1201 aatctcggac gagccgggcc tcagcctggg actctatggc ccgacatcaa tcgtcgtgcc
     1261 gattctggcc gacgctctga gcgggcgcgg tcctgagcgc atcaatcgac ctcccatcgc
     1321 ctgactgttg ctcatgaagc cggcgcgcgt ggcgcgaagg gcgctcgcgc gcggcatcaa
     1381 tcgtcagtca tgggaggtct catgtttgcg caaatggcct cgcgcttcgc cgcctttggg
     1441 cggcgtggaa aaggatcgtc cggcaccccg gttggccgcc tgctattgcc gtttgtccta
     1501 gcgcttctca cgcacgcttg taacggagca cctccggctc ctttgggggg cgccgatccg
     1561 tccgatccag gggcccgcgc gccgcgggtc ggctatcgct cgacgatcgc gccttacgat
     1621 agccagcggc cgatcgagcc cggaccttgg ggcgagcaga gccagcccgt cgttccgcaa
     1681 cccaaatacg ggcagtagga agcgccggat gctttgcgaa cctcgttcgc ttgcttttcc
     1741 gcgtcggccg cgctcaccgc gccgactgtt ctgcgcgcgg tcgagtcttg cctcggcgat
     1801 gctgctgtcc gcttgcacct ggttttcgcc ggatgccggc atgggggtcg ttgcgacgat
     1861 cacgcagcag gagctcaaca aggacgcggc tgcgatccgc tcgcccgagg aggccaaagc
     1921 cgcgagcgcc agtatccgcc gccttctcgg gcgtccgctc acagccgacg cggcggtcca
     1981 ggtcgcgctg ctcaacaacc gcggcctgca ggcggcatat gccgagctcg ccatagccga
     2041 tgccgaacgg gtcggtcaaa gccttccgcc caatcccacg tcttcctggc gccgcatcca
     2101 ggcggggccg gcactcgaga gcgagatgca actcgtcacc aatatcatcg cgcttgcgac
     2161 cctgccggcg cgatccgaga ttgccgcgga acgcttccat caagcgcagc tgcacgctgc
     2221 cgaagagacg ctccgcgtcg cccacgaggc gcggagcgcc tactatcgcg ccgttgccgc
     2281 gcgtgagctc attcagttcc tgacggaatc gcagtcggca gccgaagccg cgaccaagtt
     2341 gaccgcgcgc ctcggcgaaa ccggcgcgat gaacaagctc gatcaggcgc gcgagcaggt
     2401 gttctatgcc gatttgaccg cgcaattggc caccgcgcgc cagcgcgagg caagcgagcg
     2461 cgaggtgctc attcgcgcaa tgggcctatg ggggacaagt ctcgacttca agcttcccaa
     2521 tgcgctgccg ccagtgccgc gtcgtgttca aacgttggca gggatcgagg tggacgcggt
     2581 cgcccggcgc gtcgatctgc agatcgcccg catcgagctc gaggtgctcg cgaagtccta
     2641 tggactcacc caagccagcc gcttcatcaa catcctcgac gccggctacg ccgataagct
     2701 ggaaaagcga cccgacgaac gctttctgat gcgcgggttc gatatccgat tacaaatccc
     2761 gatattcgac ttcggcgagg ttcgcgtgcg ccaggccgaa gccacctaca tgcaggccgc
     2821 caatcggctt ctcgaaatgg cggtcaatgc gcgctccgaa gcgcgcgatg cctaccgcgt
     2881 ctatcgctcg acctacgaca tcgcggacca ttatcagcgc gaggtcttgc cgctgcgcaa
     2941 ggttatctcg gaggaaacgc tgcttcgcta caacgcgatg ctgatcgacg tgttctcgct
     3001 gctggcggag gcgcgccagc gcattgcggc gaccatcaca gccatcgaag cgaagcgcga
     3061 cttctggttg gcaacggtgg acctcaaggc ggcgatcgcc ggcggcggcc ggtcaagcaa
     3121 taagcaggaa cgagcaaacc ccctggccgt atccgctggc gacgccggcg cacgttgaac
     3181 gaggagaaga ccatgatttc gcgcagaggc tttttgggta gcgctgcggt gctggcaagt
     3241 gcgaccgcgg tgagcgggcg cgtgcaggcg gcaagcgttc ccgaggcgcc gagcaccaag
     3301 acaacgacta tgcagccgcc gatctttgcc acgggcggcc cggactacca gccggtcgtc
     3361 actctcaacg gttggacatt accgtggcgt ataaacggcg actggaaaga gtttcacctc
     3421 gttgccgagc cggtggtgcg cgagttcgcg ccaggtatga aggcgaattt gtggggttac
     3481 aatggccaag cgccgggacc gacaatcgag gcggtggaag gcgacaaagt ccgcatcttc
     3541 gtcaccaaca agttgccgga gcacacgaca gttcactggc acggcatgtt gctgcccaac
     3601 ggaatggacg gcgtcggcgg ggtaacgcaa ccccacatca agccgggaca gaccttcgtc
     3661 tacgagttcg aactcagaaa gagcgggacg ttcatgtatc atccgcactc ggacgaaatg
     3721 gtgcagatgg cgatgggcat gatgggcatg ttcgtcgtgc atcctcgcga tccgaaattt
     3781 cgccgcgtcg atcgcgactt cgtcttcatc atgagctcct acctgatcga ccccggcacc
     3841 tacctgccga aggtcgccga gatgactgac ttcaacatgt ggacgtggaa cagccgcgtg
     3901 tttccgggca tcgatccgat gccggtaagg cttggcgacc gtgtgcgggt gcgcatgggc
     3961 aatctcacca tgaccaacca tccgatccat ctgcatggcc accatttttc ggtgagttgc
     4021 acggacggcg gttgggtgcc tgagagcgcg cagtggtcgg agagcaccgt tgatgtcccg
     4081 gtcggcacga tccgagcttt cgacatgatc gccgacaacc ccggcgactg ggcgttccac
     4141 tgtcacaaga cgcaccacgc catgaatgcc atggggcacg acgtgaagaa cttcatcggc
     4201 gtagagaagc gcgatcttgc gcggcaggtc cgcaagctcg tgcccgacta catgccgatg
     4261 ggaagcgcgg ggatggctga tatggccgac atggaaatgc ctatgcccga gaacacgctg
     4321 cccatgatga cgggctttgg ccaattcggg ccgatcgaga tgggcggcat gttcaccatc
     4381 atgaaaattc gcgaagggct cgtgtccaac gattaccgcg accccggccc gtacaagaat
     4441 ctgcccggca ctgtcgccta tgaggtccga ggcactgccc cgaatgatcc gccagctcgc
     4501 gcgccggcgg cgcagcccac tccccaaaag ccgagcacaa gaccctcctc ctcgcatcaa
     4561 cactaaacgt caggacgatc tcatgatcat ctgtccttat cggctgatcg cggtggcact
     4621 gcttctcggc ttcgtggatg ccggcgccgt tcgcactggc ctgaatggtg ccgcctatgc
     4681 ccacgaaggg caccaaagct ttgccgccgg cgagcctggt gacccccaaa agcctgcccg
     4741 cactatcaag gtcgtgatgc gcgaagacgg aaagaaaatg gcattcgagc ctgcgctggt
     4801 cacagtgcac aagggcgaac agatccgctt cgttctagag aacgccggca tcgacgatca
     4861 cgagttcgtt ctcgcgactg taaaggaaaa ccgtaagcac gccgaattga tgaagaagtt
     4921 tcccgatatg gagcacgctg acccgaatgc aaaacgtctt gcgcccggcg cccatggcga
     4981 aatcctgtgg aaattcacaa aacccgggac attcgagttc gcgtgcctga tcccggggca
     5041 ccgcgaagcg ggaatgctcg gcaaggttgt tgtgaagtaa gcacgattat cccagaaagg
     5101 aggccgctat gacgggtcgc actcttcttg ccgctgtggc gctggcgttc tccctcgaca
     5161 cgagcccgca tgccgcccga gcacagtcag acgacttcgt gaacggtcaa gtcacgaagg
     5221 ttgacgagtc tgctggcaag atcacgatca agcatggtcc tctcaagaaa ttcgacatga
     5281 acgaaagcat gaccatggta ttccgcgcgg gcgatcccgc tatgctgaaa gccgtcaagc
     5341 caggggatcg catccgtttc gtgccggatc ggatcaacgg ccaattcaca gtgaagaaga
     5401 tcgagaaggc taaatgatca ggtcgggggc ggcatgtgcc gccctcacca acacctcacc
     5461 gtgtagaaaa ctacctgccg acagtcgtgc tgtcgtaagt tactcgatag agcgcgccgt
     5521 tgtagtcgtc ggagatcagc atcgagccat ccttgaggac cagtaggtca gcagggcgcc
     5581 cgatataatt gttgttttca atgaagcccg tgatgaacgg atcgaccgac ttcaccgtac
     5641 cgttctcgtt gagcgagacc acgacgatgt cgccgccgaa cttcttggat cgattccatg
     5701 agccgtggcg cgcgacgaag atagcattgc gatattccga cgggaacatg ttgccggtat
     5761 agaatcgcat gccaagcggg gctgaatgcg gccccaatag cgtgaccggg ttggtgaatt
     5821 catcgcacga gcgtccccag ccaaattcct ggtccgggat gttgccctgg tggcagtacg
     5881 gatatccgaa attgtccttg cccggttggg tgagccgatt gagcttgtcc tcgggtatat
     5941 cttccgacag ccaatcgcga ctgttttcgg tgaagtagag ctgccctaac gccgggtgcc
     6001 agtccattcc gacgacttgc cgaatcccgc gtgccacgac ttcggggccg ctgccgtcga
     6061 ggttgatacg acggagctgc gcgtgggcgg gcgacggcat gcagatattg catggtgcgc
     6121 cgacattgaa atagagcttg ttgtcgggac cgatggtgag gaatttccaa ccgtgcgcct
     6181 cgtccttggg cagatcgtca tagataacca ccggtttggg cgggttatcg aggttgtcct
     6241 cgatcttctc aaccttcgag atctttgcga gctctgcgat gtagagggtg ccgttggcga
     6301 aagcgaggcc gttcgggcga tagagtcccg agtagagtgt cttgacctcg cgtgtgccag
     6361 cctggtctgt aacggcgtag accttgtcca agatacggct gctcacg
//
LOCUS       DATLJT010000327         4069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4154270,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000327 DATLJT010000000
VERSION     DATLJT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154270"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VM910_07495"
     CDS             complement(<1..966)
                     /locus_tag="VM910_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVI62422.1"
                     /translation="MSVTQQQVLDQLAKVKSPRGVALTEGNVLSAITVNDGKVFFSIN
                     VDAHEARAWESVRAQAESVVRAIPGVTTAMIALTAERKPGAPAPAPRRPGQGVPPAAA
                     HRPPQNPAGSPMSRQSEIPGISAVIAVASGKGGVGKSTTALNLALGLRDLGLRIGLLD
                     ADIYGPSVPRLTGIREKPELNDDRKMIPIVRFGLAIMSIGFLVEEDTAMIWRGPMVMS
                     AITQMLRDVAWGTLDVLVVDMPPGTGDAQLTLAQNVPLKGAIIISTPQDLSLIDARRG
                     LAMFKKVNVPVLGIVENMSYFQCPHCGTRSDIFGHGGARHEAERLG"
     gene            complement(1108..1554)
                     /locus_tag="VM910_07500"
     CDS             complement(1108..1554)
                     /locus_tag="VM910_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI62423.1"
                     /translation="MGMSVGVLDHFNIRTRKLADTVRFYEDVLGLEKGPRPNFAFPGA
                     WMYSEGKAVVHLVDIAATDEPQKADSGVVHHVAFASRGFDGMKQRLNSRGMQFEARQV
                     PGGDLWQIFVNDPNGVMIELNYEAAKEGGTAAPEERRDDIGTKQHA"
     gene            1751..2620
                     /locus_tag="VM910_07505"
     CDS             1751..2620
                     /locus_tag="VM910_07505"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62424.1"
                     /translation="MAKVAFLGLGVMGFPMAGHLAKKGGHEVTVYNRSAAKAKQWIEK
                     FGGRSAPTPKAAAEGQQFVMCCVGNDSDLRSVTLGPDGAFSGISKGATFVDHTTASAE
                     VARELDAEAKKRGFKFVDAPVSGGQAGAENGVLTVMCGGAEDAYAAAEPIIAAYARMC
                     KRLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKKSGLDVHAVIETISKGAAQSWQM
                     ENRYKTMNDGKYDFGFAVEWMRKDLSICIAEARRNGATLPVTALVDQFYAEVEKMGGK
                     RWDTSSLLARLDR"
     gene            2876..3265
                     /locus_tag="VM910_07510"
     CDS             2876..3265
                     /locus_tag="VM910_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62425.1"
                     /translation="MSEMISEKDAAELKTRHRLNPERALPPNQHWYSLNFASVQAALN
                     YLNAPLVQSAGEVTTTARNDGTVGMLTIYPPTTPLQSPQRWAFQTFNSPAAAVNWLNE
                     PAVQSDGEVSATLRNDGTVGMFVIEPA"
     gene            3494..>4069
                     /locus_tag="VM910_07515"
     CDS             3494..>4069
                     /locus_tag="VM910_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVI62426.1"
                     /translation="MSKTADRPEVVQLPAEPIATQASSRRVAAQRVREARDRLTSTSG
                     TRPAFDRELLRQYAQTRISASYVVMLLAASTGLLFGFWMKPIAAAAWTCGILLIHGAI
                     MRACVHFLGEQTTQAATRKWRTRFVLLDLLYGLCWMSILIHPALEAVSDTVMMFLMLL
                     VIAISSMVAANLPIAALAATVPITVMIALNFV"
BASE COUNT          784 a         1289 c         1206 g          790 t
ORIGIN      
        1 cccgagccgc tccgcttcgt gccgcgcgcc gccatgaccg aagatgtccg agcgcgtgcc
       61 gcaatgcggg cactggaaat agctcatgtt ctcgacaatg ccgagcacgg gcacgttgac
      121 cttcttgaac atcgcaagtc cccgccgcgc atcgatcagc gacagatcct gcggcgtcga
      181 aatgatgatc gcacccttca gcggcacgtt ttgcgcgaga gtcaattgcg cgtcgcccgt
      241 gccaggcggc atgtcgacca ccagcacgtc gagcgtgccc caggcgacgt cgcgcagcat
      301 ttgcgtgatc gccgacatca ccatcggccc gcgccagatc attgcggtgt cttcctcgac
      361 caggaagccg atcgacatga ttgcaagccc gaagcgaacg atcggaatca ttttcctgtc
      421 gtcgttgagc tcgggctttt cccggatgcc ggtcagccgc ggcacggagg ggccatagat
      481 atcggcatcc aacaacccga tcctgaggcc gaggtcgcgc agaccgagcg cgaggttgag
      541 ggcggtcgtc gatttgccga cgccaccctt gccggaggca actgcaatga ccgcggagat
      601 gccgggaatt tccgattgcc gcgacatcgg cgagcccgcg ggattctgcg gtggccggtg
      661 cgccgccgct ggcggcacgc cctgccccgg ccgacgcggc gcaggcgcgg gagcacccgg
      721 cttgcgctcg gcggtgagcg cgatcatcgc cgtggtgacg ccggggatgg cgcgcaccac
      781 gctttccgcc tgcgcgcgca cactttccca ggcgcgcgcc tcgtgggcgt cgacattgat
      841 ggagaagaac accttgccgt cgttgaccgt gatcgccgac aacacatttc cttcggtcag
      901 cgccacgccg cgaggcgatt tgaccttggc aagttgatcc agaacctgtt gctgcgtcac
      961 gctcaaggcg catctcctgc cgaaatctca ggggcacaga taggacggtt cggcagaaat
     1021 gaacagctat tccggcaatc cagggcggat gcgtgctcgc gacatccgcc cctcggctct
     1081 cgcacgaaag gaaccgtgtt gcgcgcctca ggcgtgctgc ttggtgccta tgtcgtcccg
     1141 ccgctcttcc ggagctgccg tgccgccttc cttggcggcc tcgtagttca actcgatcat
     1201 gaccccgttg gggtcattga caaagatctg ccagaggtcg ccgcccggaa cctgacgcgc
     1261 ctcgaactgc atgcccctgg agttcaaccg ctgtttcatg ccgtcaaaac cgcggctggc
     1321 gaaggccaca tggtggacga cgccggaatc ggccttttgc ggttcgtcgg tcgccgcgat
     1381 gtcgacgagg tgcacgaccg ccttgccttc gctgtacatc catgctcccg ggaacgcgaa
     1441 attcggccgc gggcccttct ccagccccag cacgtcttcg taaaagcgga cggtgtcggc
     1501 gagcttccgg gtcctgatgt tgaaatggtc gagcacgcct acgctcatgc ccatgcgata
     1561 cactcccttt tttgtgaatt tgattggcgc gttatcctag cacgaaaccg gcaatttgcg
     1621 ccaaacgaag ccgttggccc tcaccggcgc agggggtatg gtgcacctcc cgcctccacc
     1681 accggctagt ccaacaatgc cggggagacc caggttcact caatcaagct cactcaagaa
     1741 ggcagcacac atggctaaag tcgcttttct cggtctcggc gtcatgggtt tcccaatggc
     1801 aggacatctc gcaaaaaagg ggggccacga ggtgacggtc tataaccgca gcgcggccaa
     1861 ggccaagcag tggattgaga aattcggcgg tcgcagcgcg cctaccccga aggccgccgc
     1921 cgaaggccag cagttcgtga tgtgctgcgt cggcaacgac agcgatttgc gttcggtcac
     1981 gctcggcccc gacggcgcct tttccggcat aagcaagggc gcgaccttcg tcgatcacac
     2041 gactgcctcg gccgaggtag cccgcgaact cgacgcggag gccaaaaagc gcggcttcaa
     2101 attcgtcgat gcccccgtct ccggcggcca ggccggcgcg gagaatggcg tcctcacggt
     2161 catgtgcggc ggcgccgagg acgcctacgc cgcggccgag ccgatcatcg cggcctatgc
     2221 ccgcatgtgc aaaaggctcg ggcctgcagg cagcggccag ctcaccaaaa tggtcaacca
     2281 gatctgcatc gcgggcctgg ttcaggggct gtccgagggc atccacttcg ccaagaagtc
     2341 cggcctcgat gtccatgcgg tgatcgagac catttccaag ggtgctgccc aatcctggca
     2401 gatggagaac cgctacaaga cgatgaacga tggtaagtac gacttcggct tcgccgtaga
     2461 atggatgcgc aaggacctct cgatctgcat cgccgaagct cgtcgcaacg gcgcgacctt
     2521 gccggtgacc gcgctggtcg accagttcta tgccgaggtc gagaagatgg gcggcaagcg
     2581 ctgggatacg tcgagcttgc tcgcgcggct ggaccggtag cgcctggttc caccgtcatt
     2641 gcgatgactg actgcagccg ttgcgacgcg catcgcgcgc gttggctcgc aatgacgccg
     2701 gcgcgtttcc cgatttttgc gtaccaaaat tttcaaatcc aagaacaaaa tttgcacaac
     2761 ctgaaattga tgtaagccat ttcacacgtc tcgactgctg cttggaatat tgtaggggcg
     2821 atggatgaag ggtcatccgt ccagataggt cgactcaaac ggaaggaaag atccaatgag
     2881 tgagatgatt tcggaaaagg atgcggccga acttaagacc cgccatcgtc ttaaccccga
     2941 gagggcatta ccccccaacc aacactggta ctcccttaat tttgccagtg tccaggcggc
     3001 cctgaactat ttgaatgcgc ccttggtaca gtcagcaggc gaagtcacta cgactgctcg
     3061 aaatgacggg acggtcggca tgcttactat ctacccgccg accactccac tgcagtcccc
     3121 acaacgctgg gccttccaaa ctttcaatag cccagccgcc gccgtaaatt ggttaaacga
     3181 accggcggta cagtccgatg gtgaggtgag cgcaactctc cggaacgacg gcacggttgg
     3241 gatgtttgtg atagagccag cctaagtggt tctgcttgtt ccaatcatat aacggcgaac
     3301 aaaacgggcg gtgtctctaa ccgcccgttc gacgttcaaa ctctgcgagc atttccccca
     3361 cgatttcaat gacggatttt aacctcccgt taacgagaat tcttagctct ctaagtcagc
     3421 tcttaaggat ttgttgccac agatagacaa tgcaaaaagc ccgcgcggtt cgcgggggga
     3481 cttgtgttgc ttgatgagta aaaccgccga taggcccgaa gttgtccaac ttccggctga
     3541 gccgatcgcc acacaagcga gcagtcgccg cgtcgctgcg cagcgtgtgc gcgaggcgcg
     3601 cgatcggtta acgtccacca gcggtacgcg cccggctttt gaccgcgagc tgctgcgcca
     3661 atacgcccag acccgcatct cggcctccta tgtcgtgatg ctgctggcgg cttcgaccgg
     3721 gcttttgttc ggcttctgga tgaagccgat tgcggctgcc gcctggactt gcggcatcct
     3781 gctgatccat ggcgccatca tgcgcgcctg cgtccacttt ctcggcgagc agaccacgca
     3841 ggccgcgacc cgcaaatggc ggacgcgctt cgtgctgctc gatctgctct acggcctgtg
     3901 ctggatgtcg atcctgatcc atccggcgct cgaggccgtc tccgatacgg tgatgatgtt
     3961 cctgatgctg ctggtcatcg caatctcgag catggtcgcg gccaacctgc cgatcgcagc
     4021 gctcgcggcg accgttccga tcacggtgat gatcgcactc aatttcgtg
//
LOCUS       DATLJT010000328         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2086232,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000328 DATLJT010000000
VERSION     DATLJT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086232"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="trmFO"
                     /locus_tag="VM910_07520"
     CDS             complement(<1..939)
                     /gene="trmFO"
                     /locus_tag="VM910_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HVI62427.1"
                     /translation="MTRRMSAVHIIGGGLAGCEAAWQLARRSVPVVLHEMRPVRATAV
                     HKTAQLAELVCSNSFRSDDAQGSAIGLLHAEMRRLQSLIMQAADANKLPAGSALAVDR
                     DGFAGAITAALDAEPLIEIRREEIVIPPDGWDSVIVATGPLTSPTLSESIRSLTGEDA
                     LAFFDAIAPIVYRDSIDMQVAWFQSRYDKTGPAGSGADYINCPFTRSDYDNFIDALLA
                     AEKSTSHDFETATPYFDGCLPIEVMAERGRETLRHGPMKPFGLTNPHCEIKPHAVAQL
                     RQDNRLGTLFNMVGFQTKLKHGEQSRIFRTIPALANA"
     gene            1118..2167
                     /locus_tag="VM910_07525"
     CDS             1118..2167
                     /locus_tag="VM910_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HVI62428.1"
                     /translation="MAAAFDVEILSLATANPPFRLSQEEAAVYAKQLYPQLSKLWQLY
                     DNTGIECRYNCEPIEWYLKPRSWQERTTAFRQHALDLLEEITIKTTERAGVALNDVDM
                     IVTNTVTGLAIPSLDALLLNRLKLAPTIERLPMFGLGCGAGVAGLSRAARLAHAKDGA
                     LVLFLTVDLCSLCLRVNDPSPAMFVSTALFGDGAAGLLLHNTRSGARSGPRILTSGEW
                     CWGNTEHIMGWDIKDDGFGIVLSPELPSLMKKALAPALNDFLERNGLALGDFNGFLFH
                     PGGRKLLETMEEVLGLTRDQLSHSWEVLRRYGNMSSATALFVLEDAIQRGACGPHLLA
                     AFGPGFSAYFAAVEL"
     gene            2164..2679
                     /locus_tag="VM910_07530"
     CDS             2164..2679
                     /locus_tag="VM910_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018899585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI62429.1"
                     /translation="MIEFGWGAALLTFLTLQRIAELWWARQNERRLFAAGGVEYGRSH
                     LVLIVLVHAAWLIGMWALAYDRPVEPLFLVIVVLFQIGRFWVLGTLGRRWTIRIIVVR
                     GEKLVTRGPYRWLCHPNYVVVTGEIAAVPLALGLPLYALAFSVLNAVVLAIRIPAENA
                     ALAAGACGRTR"
BASE COUNT          533 a          815 c          886 g          590 t
ORIGIN      
        1 cgcgttggcc agagccggaa tcgttcgaaa gatgcgcgac tgctcgccat gtttgagctt
       61 ggtctgaaaa cccaccatgt tgaagagggt gccgagccgg ttgtcctggc ggagctgcgc
      121 gaccgcgtgg ggtttgatct cacaatgagg atttgtcagc ccgaacggtt tcattgggcc
      181 gtgccgcagc gtttcgcgcc cgcgctcggc catcacttcg atcggcaggc agccatcgaa
      241 atatggcgtc gccgtttcga agtcgtgaga tgttgatttc tcggcggcga gcaatgcgtc
      301 gataaaattg tcgtagtcgg agcgtgtgaa cggacagttt atataatcag cccctgaacc
      361 ggccggaccg gtcttgtcat aacgcgattg gaaccaggcg acttgcatat cgatcgagtc
      421 gcgataaacg atcggcgcaa tagcatcaaa gaaagccagc gcgtcttcgc cggtcagtga
      481 acggatggat tcgctcagtg tcggcgaggt cagcgggccg gtggcgacga tgacgctgtc
      541 ccagccgtcg ggaggaatca cgatttcttc gcggcggatc tcgatcagcg gctcggcatc
      601 gagcgccgcc gtgatggccc cggcaaagcc atcgcgatca acggcaagcg cgctacctgc
      661 gggcagctta ttcgcatcgg cggcctgcat aatcagagat tgaagccgcc gcatctcggc
      721 atggaggagg ccaatggcac tgccctgcgc atcgtccgat cggaatgagt tcgaacaaac
      781 gagctcggct agctgcgcgg tcttgtggac cgcggtcgcg cgcactggac gcatttcgtg
      841 caacacgacg gggacgctcc ggcgcgcgag ctgccaagcg gcctcacagc cggctaaacc
      901 gccaccgatt atgtgaacgg cgctcatcct tctcgtcatt ttggtaagct aggccgctaa
      961 ccgcttgacc acaaccgcac gggaataaat cagcggctgc gccgctatac gagaatacgg
     1021 acgatccgac gaaatccggt gttagtcatc tcagtagtga gcgccaagga acccctgcat
     1081 gcttgatgta gtcaggtccg cagcacatcg gcgggccttg gcggcggcgt ttgacgttga
     1141 aatcctgtcg cttgcgaccg ccaatccgcc attccgtttg tcgcaagagg aagcggccgt
     1201 ctacgccaag caactatacc cgcaactgag caaactgtgg cagctctacg acaatactgg
     1261 gatcgagtgc cgctacaact gcgagccgat cgagtggtat ttaaagccgc gtagctggca
     1321 agagcgcacc acggcgttcc gccagcatgc gctcgatctc cttgaggaaa taaccatcaa
     1381 aacgacggag agggccggag tcgcactcaa cgacgtcgat atgatcgtga ccaatacggt
     1441 caccggactt gccattccaa gtctcgatgc actgctgctg aacaggctca aactggcgcc
     1501 aacgattgag cgattgccaa tgttcgggct cggctgtggc gccggagtgg cgggcctgtc
     1561 ccgcgcagcg cgtctcgctc acgccaagga tggcgcactg gtcttgtttc tgacagtgga
     1621 tctctgcagc ctgtgtctgc gtgtcaacga tcccagcccg gcgatgtttg tctccaccgc
     1681 gctgttcggc gacggtgctg caggcctgtt gctgcacaac accagaagtg gcgcgcggtc
     1741 aggaccgcgc attctgacaa gcggagaatg gtgctgggga aataccgagc acatcatggg
     1801 ctgggacata aaggacgacg gcttcggcat cgtgctcagc ccggaattgc catcactgat
     1861 gaaaaaagca ctggcgccgg cgctcaatga ttttttggaa cgcaatggac ttgcgctcgg
     1921 cgactttaac ggcttcctgt ttcaccccgg tggccgcaaa ttgctcgaaa ccatggagga
     1981 ggtgctgggg cttacccgcg accagctcag ccactcttgg gaggtgttgc gccgttacgg
     2041 aaatatgtcc tcggcgacgg cgttgtttgt tctggaagac gccattcagc gcggagcatg
     2101 cggcccgcat ttgctggcgg cgttcggtcc aggtttctcc gcgtacttcg ccgcggtgga
     2161 gctgtgatcg aattcggttg gggcgcggcg ctcctcactt ttctcacgct ccagcggatt
     2221 gccgagttgt ggtgggcgag acagaacgaa cgcaggttgt tcgctgccgg cggcgtggaa
     2281 tacggccgat ctcaccttgt gctgattgtg cttgtccacg ccgcttggct gatcggaatg
     2341 tgggccttgg cctacgatcg cccagtcgag ccgctgtttt tggtcatcgt ggtgctcttc
     2401 caaatcgggc ggttttgggt actggggacg ctcggacgcc gctggacgat tcgcattatc
     2461 gtcgttcgcg gggaaaagct ggtgacgcga ggtccttacc gatggctgtg tcatccgaat
     2521 tatgtggtgg tgaccggcga gatcgcggcg gtgccgctgg cgctcggact gccgctttat
     2581 gcgctcgctt tctccgttct caatgccgtg gttcttgcga tccgcattcc ggcagaaaat
     2641 gcagcgttgg cggcaggcgc ctgcggacgg acccgctaaa tcgatcggcg ctgttggcgc
     2701 gcgttaggcc ctctgctata ggaccgcgcg tgcacccctc taaggcagcg gttcatgtca
     2761 gtcgataacg acaccgtccg ccgtgtcgcc catcttgccc gaatcggggt cgcggagggc
     2821 gaag
//
LOCUS       DATLJT010000329         5721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2086278,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000329 DATLJT010000000
VERSION     DATLJT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2086278"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VM910_07535"
     CDS             complement(<1..1247)
                     /locus_tag="VM910_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI62430.1"
                     /translation="MPNAQLQPVLDRIDADFDDSLERLFALLRIKSISADPAFAKDCK
                     AAADHLAREIASLGFTTEVRATAGHPAIVARSNEKGNGHGTARPRVLFYGHYDVQPVD
                     PLDLWHRPPFEPVVTAHADGRKIIVARGAEDDKGQLMTFVEACRAWKKVTGSLPLEIT
                     ILIEGEEEVGSKNFVPFLEANKDELKADYALVCDTGMWDPDTPAITTSLRGLVYDEVK
                     IKAASRDLHSGVFGGGAQNPIRVLTRILGGLFDDNGHITIPGFYDGVKDLPPDILAQR
                     KKLNLTPEMFLKPIGLSIPAGEKDRLLIEQVSSRPTCDVNGIVGGYIGEGSKTVIPAE
                     ASAKISFRLVEGQDPAKIRKAFRDYVTARLPADCGVEFGDHSTAPAIALPWNMKPLAA
                     ARKALTEEWGKEALLIGSGASIP"
     gene            complement(1276..2595)
                     /locus_tag="VM910_07540"
     CDS             complement(1276..2595)
                     /locus_tag="VM910_07540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HVI62431.1"
                     /translation="MTSQPSNPFRLPSLRAPLDVLFLLCCVVLTAFVLGPEIFGNGKS
                     KDYALWFWAGQQVLHGGDLYPSDPHDYFSFLYPPLPAVLLAVPSWFGKVVLYTCLSLL
                     NCVAWWMTGQFSNAMTGSGKRPGPLLFALPGVLTITSVFDMFDLGQPNLVLLALMLFG
                     FWLLQQEKPWIAGSMFALAAAIKVFPAAVLPYLVWRRQWATVASMAICLCVFLFVVPA
                     PVRGFQHNIVELKTWYRGMVGSSSERGFGQRDAQNWSWVNQSIVAMTHRFTRPINYNQ
                     EDPAKPVRTMNIVDISYEAANRIVLAVYAAFGIGFIAIMPRASRRTRRSDAEEIGILF
                     CLMTLATPLARQYYFISLFFPMTVVIHRAAFDPRPKARFWSWAALVTTVLLMCLSFRI
                     FPYELQAYGNNFLATLVLAGCLVWHILHPPTEEAASSTAPAELKGKA"
     gene            complement(2622..4235)
                     /locus_tag="VM910_07545"
     CDS             complement(2622..4235)
                     /locus_tag="VM910_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HVI62432.1"
                     /translation="MATSETSVADTNEKIFVGKGEQPAWLTLVLANRHGLVTGATGTG
                     KTVTLQVMAEGFARAGVPVFAADIKGDLSGISEVGEAKDFVVKRAAELGFSFQPDQFS
                     AVFWDVFGEQGHPVRATVTEMGPLLLARMLDLNDVQEGVLNVAFRVADENGLALIDMK
                     DLRALLDAIVPDAKKGAEEENDPLAPIRKAAQGFGNVSRATVGTIQRQLLVLENQGGT
                     KFFGEPALSLKDFMRTDRDGRGMVNILVADKLMQSPRLYATFLLWMLSELFEELPEVG
                     DPPKPKLVFFFDEAHLLFNDAPKALMDKIEQVVRLIRSKGVGVYFVTQNPIDVPDRVL
                     GQLGNRVQHALRAFTPRDQKAVQAAAQTFRPNPKLDTAKAIMELGKGEALVSFLEGNG
                     IPAMVERVLIRPPSARIGPITPEERKAIMDASPVKGKYDTAIDAESAYEILQKRVAGT
                     AAPADAPAGGGGILGHIGAIVSTIFGTNVKRGRLSTGQLIARNVTRSVTDKVVGGAVA
                     DIGKQVGGSIGGSVGRALVRGALGGLLRR"
     gene            4560..4841
                     /locus_tag="VM910_07550"
     CDS             4560..4841
                     /locus_tag="VM910_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HVI62433.1"
                     /translation="MDELVGKLATKAGIDSVVAEKTVGISVDFLRREDTSENVRAQIA
                     VGTRLMGLGIGIRKIQSGAREFVRFGQDKIGAAQMSAIIAGTPGTSQFA"
     gene            4866..5273
                     /locus_tag="VM910_07555"
     CDS             4866..5273
                     /locus_tag="VM910_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HVI62434.1"
                     /translation="MTYPLSELEGMTAFSSSKLKSLGIRTTDALLEAARTVKGRKALA
                     ARSGISEQQLLEWANVSDYMRIPGMGRAKVGLVRAAGVTTVRELALRNPARLAQNMRE
                     VNTRRKLVRVLPSEKSVEQLIAQARKLQPKISY"
     gene            5383..>5721
                     /locus_tag="VM910_07560"
     CDS             5383..>5721
                     /locus_tag="VM910_07560"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVI62435.1"
                     /translation="MTATTSRPASPTAIPAAHPVLRALLTKPYPAVMGVLNITPDSFS
                     DGGRFLAPERAVAQARRMIAEGADIIDVGAESTRPYGGMQPIPAEQEVERLQPVLAQI
                     VALGVPVSIDS"
BASE COUNT         1143 a         1822 c         1711 g         1045 t
ORIGIN      
        1 atcgggatcg atgccccgga cccgatcagc agcgcttcct ttccccattc ctccgtcagc
       61 gccttcctcg ccgcggcgag cggcttcatg ttccagggga gcgcaatcgc gggcgcagtg
      121 gagtggtcgc cgaattcgac cccgcaatcg gcaggcaggc gtgccgtcac gtaatcgcgg
      181 aaggcctttc ggattttcgc tgggtcctgg ccctcgacca ggcggaagga gatctttgcg
      241 gacgcttccg ccgggatcac ggtcttggag ccttctccga tatagccgcc gacgatgccg
      301 ttgacgtcgc aggtcggccg cgaggagacc tgttcgatca gcaggcgatc cttctcgccg
      361 gccggaatcg aaagtccaat cggcttcagg aacatctccg gcgtcaggtt gagctttttc
      421 cgctgcgcca gaatatccgg cggcagatct ttcacgccgt cataaaagcc gggaatggtg
      481 atgtggccgt tgtcgtcgaa caacccgccc aggatacgcg tcagcacacg gatcggattc
      541 tgcgcaccgc cgccgaacac gccggaatgc aggtcgcggc tcgcggcctt gatcttgacc
      601 tcgtcataga cgagcccgcg cagcgaggtc gtgatcgccg gcgtatccgg gtcccacatg
      661 ccggtgtcgc agaccagggc gtagtcggcc ttcagttcgt ccttgttggc ttcgaggaac
      721 ggcacgaaat tcttcgatcc gacctcttcc tcgccttcga tcagtatggt gatctcgagc
      781 ggcagcgagc ccgtcacctt cttccaggcg cgacaggctt cgacgaaggt cataagctga
      841 cccttgtcgt cctcggcacc gcgcgcgacg atgatcttgc gcccgtcggc gtgggcggtc
      901 accaccggct cgaagggcgg gcggtgccac agatcgagcg gatcgaccgg ctgcacgtca
      961 tagtggccgt aaaacagcac gcgcggccgt gccgtgccgt gaccgttgcc cttctcgttg
     1021 cttctggcga cgatcgcggg atggccggca gtcgcccgga cctcggtcgt aaagccgagc
     1081 gaggcaattt ctctcgcaag atggtcggcg gctgccttgc agtccttcgc gaaggccgga
     1141 tcggcggaaa tcgacttgat ccgcaacaac gcgaacagcc gctcgaggct gtcgtcaaaa
     1201 tcggcatcga tccggtcgag caccggttga agctgggcat tgggcatttt tctcgttccc
     1261 tgcgtagagg ggtgtctagg ctttgccttt tagctccgca ggagccgtcg agctagccgc
     1321 ctcctccgtc ggcggatgca gaatgtgcca gaccaggcaa ccggccagca caagagttgc
     1381 aaggaagttg ttgccgtaag cctgcagttc atacgggaaa atgcggaacg acaggcacat
     1441 cagaagcacc gtggtcacga gcgccgccca gctccagaac cgggccttcg gacgcggatc
     1501 aaaagccgcg cgatggatca cgacggtcat cgggaagaac agggaaatga aataatattg
     1561 gcgggcgagc ggcgtcgcca atgtcatcag gcaaaacagg atgccgattt cctcggcgtc
     1621 cgatcgccgc gtccggcgcg aagccctcgg catgatcgca atgaaaccga tcccgaacgc
     1681 tgcatagacg gccagaacga tccggttcgc cgcctcgtac gagatatcga cgatattcat
     1741 ggtccgtacc ggcttggccg ggtcttcctg gttgtagttg atcgggcgcg taaatcggtg
     1801 cgtcatcgca acgatggatt ggttgaccca cgaccagttc tgcgcgtcgc gctggccaaa
     1861 tcccctttcg gagctggagc ctaccattcc gcgataccag gtcttcagct cgacgatgtt
     1921 gtgctggaaa ccgcgcaccg gcgcggggac aacgaaaaga aatacgcaaa ggcagatcgc
     1981 catgctcgcg accgttgccc actggcggcg ccagacgaga tagggcagca cggctgcggg
     2041 gaaaaccttg atcgcggcgg cgagcgcaaa catgctgcct gcaatccatg gcttctcctg
     2101 ctgcagcaac cagaatccga aaagcatcag cgcgagaagc acgagattcg gctgcccaag
     2161 atcgaacatg tcgaaaaccg aggtgatggt gagaacgccg ggcagcgcga acaacagcgg
     2221 tccgggcctc ttgccggagc ctgtcatcgc gttcgagaac tgccccgtca tccaccacgc
     2281 aacgcagttc agcagtgaca gacaagtata aaggacgact ttgccgaacc agctcggaac
     2341 ggcgagcaaa accgccggaa gcggcggata gaggaaactg aagtaatcat ggggatcgct
     2401 gggatagagg tccccgccgt gcagcacctg ctggcccgcc cagaaccaga gcgcatagtc
     2461 cttgctcttt ccattgccaa agatttcggg gcccagcacg aacgccgtaa gcaccacgca
     2521 gcagagcaga aacagcacgt cgagcggggc tcgcagcgac ggaagtctga acggattcga
     2581 tggttgcgat gtcacgcgaa actacgccag caattgagag gtcatcgccg cagcaaaccg
     2641 ccgagcgcgc cgcgcaccag cgctcggccg accgagccac cgatcgaacc gccgacctgc
     2701 ttgccgatat cggcaaccgc cccgccgacc actttgtcgg tgaccgagcg ggtgacattg
     2761 cgcgcgatca actggccggt tgacagcctg ccgcgcttga cattggtacc gaaaatcgtg
     2821 ctgacgatcg cgccgatatg gccgaggatg ccgccgccgc cagccggagc atccgcaggc
     2881 gcggctgtgc cggcgacgcg cttctgcaag atctcgtagg cggattcggc atcaatcgcc
     2941 gtgtcgtatt tgcccttcac cggactggca tccataattg ccttgcgttc ttccggcgtg
     3001 atcggtccga tccgcgccga tggcggccgg atcaggacgc gctcgaccat cgccgggatg
     3061 ccattgcctt ccaggaagga caccagcgcc tctcccttgc cgagttccat gatggccttg
     3121 gcggtgtcaa gctttggatt gggccggaac gtttgcgcag ccgcctgaac cgccttctgg
     3181 tcgcgcggag tgaaagcgcg cagtgcatgc tgcacgcggt tgccgagctg gcccagcacc
     3241 ctgtccggca cgtcgatcgg attttgcgtc acgaaataga cgccgacgcc cttggagcgg
     3301 atcagccgca ccacctgttc gatcttgtcc atcagcgcct tcggcgcatc attgaacaac
     3361 agatgggctt cgtcgaagaa aaagacgagc ttcggtttcg gcggatcgcc gacctccggc
     3421 agctcttcga aaagctccga cagcatccaa agcaggaacg tcgcgtaaag ccgcgggctc
     3481 tgcatcaatt tgtcggcaac gagaatgttg accatgccgc gtccgtcgcg gtcggtccgc
     3541 atgaaatcct tgagcgacag cgcaggctcg ccgaaaaact tggtgcctcc ctggttttca
     3601 agcaccaaaa gctgacgctg aatggttccg accgttgccc ggctgacatt gccgaaacct
     3661 tgcgccgctt tccggatcgg cgccagcgga tcattctcct cctcagcacc cttcttcgcg
     3721 tccgggacga tcgcatccag aagcgcccgc agatccttca tatcgatcag cgcgagcccg
     3781 ttttcgtcgg cgacacgaaa cgcgacgttc agaacacctt cctgcacgtc gttgagatcg
     3841 agcatgcgcg ccaacaaaag cggccccatc tcggttaccg tcgcacgcac cgggtgacct
     3901 tgctcgccga atacgtccca gaacacggcg gagaactggt cgggctggaa gctgaagccc
     3961 aattcggcgg cccgcttcac gacgaaatcc ttggcttccc ccacttccga aatgcccgac
     4021 aagtcgccct tgatgtcggc ggcgaaaacc ggcacgccgg cgcgggcaaa cccttccgcc
     4081 atcacctgca acgtgacggt cttaccggtt ccggtcgcgc cggtgacgag cccatggcga
     4141 ttggccagca ccagggtcag ccaggccggc tgctcgccct tgcccacgaa aatcttctca
     4201 ttcgtgtcgg ccacgctcgt ctccgatgtc gccattgcaa tccgcctttc actcgtctcc
     4261 ggcgcggcat catactgcat gtcgcgaggc gattaaaacc ggctgcccct cgttcgaacg
     4321 cgcggttttg catatgctca gccggaatgc tctgcttcgc cctccgtcga aagactgaat
     4381 cgaccgtccg ttcgggacag tttttcacgc ttttcaccgc agcccttgca ttgatgcaac
     4441 actcgcaatg cgaaacgcgc tttccaatgt ggtgatcgct tgtatttcat tccttcgaag
     4501 ctcaagatgt cgcctcaagg cagggagacc cggtaagaac cggtcgaggg cggggcaata
     4561 tggacgaatt ggtggggaag ctggcgacaa aagccggcat cgatagtgtt gtcgctgaaa
     4621 aaaccgtcgg catcagcgtg gatttcctcc ggagggagga tacttccgaa aatgttcgtg
     4681 cgcagatcgc ggtgggcacc aggctcatgg gcctcggcat tggcatccgc aaaattcaga
     4741 gcggcgcgcg cgaatttgtc aggttcggcc aggacaaaat cggagcggct caaatgagcg
     4801 cgatcattgc ggggacacct gggacttctc agttcgccta ggcacatttt ttaatatcga
     4861 ctgctatgac atatccactt tccgagctcg aaggcatgac ggccttctct tcctccaagc
     4921 tcaaatcgct tggtattcgc accaccgacg cactgctcga agctgcccgc accgtgaagg
     4981 gtcgaaaagc gctcgcagcc aggagcggta tcagcgagca gcagcttctg gaatgggcca
     5041 acgtatccga ctacatgcgg atccccggca tgggaagggc caaggtcggg ctggtgcgtg
     5101 ccgccggcgt gaccacggtc cgcgaactcg cgctgcgcaa tccggcgcgc cttgcccaga
     5161 acatgagaga ggtgaatacc aggcgcaagc tcgtccgcgt gctgccgtcc gaaaagtcgg
     5221 tcgaacagct catcgcgcag gcgcgcaagc tgcaacccaa gatcagctac tagccgggca
     5281 aaaacaccgt cccggcggta cgccgactcc ccttggccgg gaagacgagg ccacccctgc
     5341 gggcatcttg actcccatgc cgcgaccgcg caaagcgacc gcatgaccgc gaccacgtcc
     5401 agacccgctt cgccgacggc gatcccggcg gctcatcccg tgctgcgggc gctgttaacc
     5461 aaaccctatc cagccgtaat gggcgtactg aatatcacgc cggattcgtt ctcggacggc
     5521 gggcgatttc tggcacccga gcgcgcggtg gcccaagcgc ggcggatgat cgccgaaggt
     5581 gccgatatca tcgacgtcgg cgctgaatcg acccgccctt acggcgggat gcagccgatc
     5641 ccggccgaac aggaggtgga acgcctgcag cctgtgctgg cgcaaatcgt tgcgctcggc
     5701 gttcccgtct ccatcgacag c
//
LOCUS       DATLJT010000330         5005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3005315,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000330 DATLJT010000000
VERSION     DATLJT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005315"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..381
                     /locus_tag="VM910_07565"
     CDS             259..381
                     /locus_tag="VM910_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62436.1"
                     /translation="MRKFIAALALISLIAIPSLTVTANAAPVSPSSSSFGSNGY"
     gene            665..925
                     /locus_tag="VM910_07570"
     CDS             665..925
                     /locus_tag="VM910_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62437.1"
                     /translation="MKRVFAAVLATACIVASTPGFARGAVGHGGGFATHGGFVRAPST
                     LLGSPAPQTPAFENRIPAPLAAPAQPPVINGPSARSPYGGVL"
     gene            complement(1162..2769)
                     /locus_tag="VM910_07575"
     CDS             complement(1162..2769)
                     /locus_tag="VM910_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HVI62438.1"
                     /translation="MAKTAAAVPEEQHFILSTLSPSLAQRRLALAVVLALLVAFVTTA
                     GPLSTIQPGRIGAFVPAYATAIFVTDLLTAVLLFAQFSILRSRGLLAIASGYLYTALI
                     VIPWMLTFPGVFTPSGLLGAGLQTTTWLYTLWHAGFPLFVIAYAILKDADPAEGLWQG
                     SAGVAILSSVAVTAAVVCAATFLVTAGNDLLPRESLDPIQFSTVWLYVAGCLALLILV
                     ALIVLWIRRRSVLDLWLMVVMCAYLIEIYLISFPVPVRYSIGWYAGRVFGLVSGSLVL
                     FVLLYEIMMLYAQLLRAVLVQRREREARLMTGDAVAATIAHEVKQPLSGMITNADAGL
                     RWLDRPTPDLDEAKAAFKQIAADGHRAGAVIGSIRAIFKQDARSRTSLDISDLIGEAF
                     VLLRGDLQKHRVLVQTEADKRLPQVLGDRIQLQQVLLNLITNAIESMADEDGPRILSV
                     KCEVQDGGVMVSVADTGAGIGAQDVGRIFNPLFTTKSGGMGMGLSICRSIIEAHDGRL
                     LVAPNAPRGSVFQFILRAGTAASAAAS"
     gene            complement(2909..3055)
                     /locus_tag="VM910_07580"
     CDS             complement(2909..3055)
                     /locus_tag="VM910_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62439.1"
                     /translation="MPGPVLGHTPHHDGVKRDAKEPAVIALFGIAPVDLTLVDPGRPG
                     SRQV"
     gene            3186..4169
                     /locus_tag="VM910_07585"
     CDS             3186..4169
                     /locus_tag="VM910_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62440.1"
                     /translation="MIRSAVGKLASVVAIAALALIVMPQAAQSQGFPSKPVRIIVPYT
                     PGSPNDVLARLLAQPLQGKLGQAVVIDNKPGGGTTIGSKTAAVAPPDGYTLLFASSAL
                     VIEPILKKQVEYDPLKDFTPIAFVARTSSMLTVNAQVPANSANEFVTYAKDNPGKLSV
                     GFAQGTVSQLLAEYFNHLHGLDITSVPYRGGALVIPDMLGGRIHVYWPTPATVLPLIR
                     EGKIKALAISSPQRNPDLPDVPTMKELGLDELTLEFWAGMWAPAGVPADVVEKLNAAV
                     NAALQSSETIASMKKLGFELRIGSSQDFAAFVAAEIPRWTTVVKASGVKSQ"
     gene            4219..>5005
                     /locus_tag="VM910_07590"
     CDS             4219..>5005
                     /locus_tag="VM910_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI62441.1"
                     /translation="MSTSKKVVVVGAGIGGLTAALALLKRGIDVEVYEQSDQLKEVGA
                     GIQISSNGTRVLFALGLETALANVQVRPERRELRHWSTGETWNWFDLGDKSIERFGTP
                     HLMLHRADLHGLLADAVRSLKPRAIRLNKRCVSVVSHAGHVEATFEDGGTVKAPYLIG
                     ADGIHSKVRVCLFGPSKPIFTACIAWRGLIPMEKLPPRLARMVGTNWLGPRGNVLHYP
                     VRRGEIMNFVSTSERDDWQVESWSTVGTKEELRNDFRDWHADVQ"
BASE COUNT         1011 a         1565 c         1524 g          905 t
ORIGIN      
        1 agcgacttcc ttgcagagag ttaatgcggc ccaagcgtcc aagcaccttg cggcggcttt
       61 cctcgctcgc cgggccgaaa cggcaagagg ccaaaccaaa gtattagcgg ctcatcggtt
      121 cggttatcca atcgatccag acatatactt atccggtcac tcccgaagac ctatctccgt
      181 gatgactgca aggcatggcg atccgccgtg ttgttgctac agaaaacagg acaacgttcc
      241 gaaaaaagga gatgtgtcat gaggaaattc atcgccgctc tcgcgttgat ctcgctgatc
      301 gcgatcccgt cgctcacggt gactgcaaac gcggcccccg tgtccccgtc gagctcctcg
      361 ttcggcagca acggctacta acaatttcgt gggcaagcga gcgtttctgt tcgcggcctg
      421 gcattcgaga gggcggccgg cttgcacgcg cgagtcggcc gtcctctcgc tacaagatca
      481 atcttgccga taaagcgctt gtgttcgccg cggtgcctgt cgatgagcct catcactggc
      541 gcggttggat gtacgaatga agcgcaagag cggtcaaagc aacgcaaggc agattaaaag
      601 tcgttgcccg gggatgatta gatgaatgac gtcgaggggc ggcgcaagat gaggagtaac
      661 gaccatgaaa cgcgtttttg ccgcagtatt ggcgacggct tgcatcgtcg catcgacgcc
      721 cggctttgcc cgcggggcgg ttgggcatgg tggcgggttt gcgacccatg gcggctttgt
      781 gcgcgctcct agcactctct tgggatcacc agcgccgcag acgcccgctt ttgaaaaccg
      841 gattccggcg ccgctcgcgg cacctgcgca gccgcccgtc atcaacgggc cgtcggcgcg
      901 cagcccgtac gggggagttc tgtagccgag ccgctccctc cgttgagcgg ccgtgctgag
      961 tattgcttac agcagggcgg cggcgtgggc agcaacgctc gctccgccta gcctgctgcc
     1021 attttgctac tagcacctcc ggttcatgca ggtgccgctc gccggcgtga tcttggcggg
     1081 atcttggcga gctggctgat cgccaggcgc gctattggga ataacattcg aatcaacctc
     1141 gtagaaatcg gcagagcgaa gtcacgaagc cgcggcggat gccgccgtac cagcgcgcag
     1201 gataaattga aatacggagc cccgaggggc gttcggagcg accaacaagc ggccgtcatg
     1261 ggcctcgata atggagcggc agatcgacag gcccattccc atgccgcctg attttgtcgt
     1321 gaacagcggg ttgaatatcc gaccgacatc ttgcgcgccg attccagctc cggtatccgc
     1381 cactgacacc ataacgccgc catcctggac ttcgcattta accgatagga tgcgaggtcc
     1441 gtcctcgtcg gccatcgact cgatggcatt cgtgatcaga ttgagaagca cctgctgcag
     1501 ctggattcga tctcctagta cctgcggcag gcgcttgtcc gcctcggttt gcacaaggac
     1561 ccggtgcttc tgcaggtcgc cgcgtagcaa aacaaaggct tcgccgatga ggtcgctgat
     1621 gtcgagcgag gttctgctcc gggcatcctg cttgaagatc gctcgaatgc ttccgatcac
     1681 cgctcccgcg cggtgcccat cggcggcgat ttgcttgaat gctgccttgg cttcatcgag
     1741 atccggcgtc gggcgatcaa gccaacgcaa gcccgcatcg gcattcgtga tcatcccgga
     1801 caacggctgc ttgacttcgt gggcgatggt ggccgcaacc gcgtccccgg tcatcagccg
     1861 cgcctcgcgc tcgcggcgtt ggacgaggac cgcgcgcagc agctgcgcgt agagcatcat
     1921 tatctcgtac aggagcacaa acaggacgag acttccggac acgagcccga agactcggcc
     1981 ggcataccaa ccgatgctgt agcggaccgg aacgggaaaa gagatcagat agatctcgat
     2041 caggtacgca cacatcacca ccatcagcca cagatccaag accgagcgcc gacggatcca
     2101 aagcacaatg agggcaacaa ggatcaacag ggctagacat ccggcaacat aaagccagac
     2161 ggtagaaaac tggatcgggt cgagcgactc gcgaggcaat aggtcatttc ccgctgtgac
     2221 aagaaatgtc gctgcacata cgacggccgc ggtcactgcc acgcttgaaa ggatggccac
     2281 accggcggaa ccctgccaca gcccctcggc cggatctgca tccttcaaga tggcataggc
     2341 aatgacgaac aaaggaaaac cggcgtgcca cagagtatag agccaggtcg tggtctgcag
     2401 cccggcgcca agcaagccac tgggcgtgaa cacgcccgga aaggtcagca tccatgggat
     2461 cacgatcagc gccgtgtaga gatatccgct cgctatcgcg aggaggccgc gcgaccgcag
     2521 gattgagaac tgggcgaaca acagcacagc cgtgagcaaa tcggtcacga agatcgccgt
     2581 ggcgtaggcc gggacaaagg cgccgatccg tcccggttgg atggtcgaca gcggcccagc
     2641 cgttgtgaca aaggcgacga gcaaggctag cacgacggca agggcgaggc gtcgctgggc
     2701 caggctcggc gataaggttg agaggatgaa atgctgttct tccgggacag cagccgccgt
     2761 cttcgccatc ggcgcgaccc ccgcaggggc tggagcacac ggaaccaatc ttattccgcg
     2821 tcgcggccct tcacatgaga ccgccggctc acagtcacgc ccattctgtc acttggagcg
     2881 gcaggtgaca acgcatgacg cggttggatc aaacctgccg cgagcccggc ctacccggat
     2941 ccaccagcgt gagatcgacc ggcgcaattc caaacagcgc gatcaccgct ggttccttcg
     3001 cgtcgcgctt cacgccgtca tggtgcggag tgtgtcccag gaccggcccc ggcatgacct
     3061 ttcagtttac gatttcggca tatgggcaag gcggctgatg cgctcgaaca ggccaccgag
     3121 ttggccgctt tgtgctgctc cttcgagctt tgaaattcgc taatattcgc cacggagcac
     3181 agggcatgat ccgatccgcg gtcggaaagc ttgcatccgt cgtcgcgatc gcggcgcttg
     3241 ccctgatcgt catgcctcag gcggcacagt cccaaggctt tccgagcaag ccagtccgga
     3301 tcatcgtgcc ctatacgccg ggctcgccga acgacgtcct cgcgcgcttg ctggcgcagc
     3361 cgctgcaagg caagctcggc caggcggtcg tgatcgacaa caagcccggt ggcggcacga
     3421 cgatcgggtc caagacggcc gcggtcgcgc ctcccgacgg ctacacgctg ctgttcgcaa
     3481 gctcggccct cgtcatcgag ccgatcttga agaagcaggt cgaatacgat ccgctgaagg
     3541 attttacgcc catcgccttc gttgcacgca cctcgtccat gctcaccgtc aacgcacaag
     3601 tgccggccaa ttcggcgaac gaattcgtca cctatgccaa ggacaatccc ggcaagctca
     3661 gtgtcggctt tgcgcagggg acggtgtcgc aactgctcgc cgaatatttc aatcacctgc
     3721 acgggctcga tatcaccagc gtgccgtacc gcggaggcgc actcgtcatt cccgacatgc
     3781 tgggcggccg catccacgtc tactggccga cgccggcgac ggtgctgccg ctcattcgcg
     3841 agggcaagat caaggcgctg gccatatcga gcccgcaacg caatcccgat ttgcccgacg
     3901 tgcccaccat gaaggaactc ggcctcgacg aactaacgct tgaattctgg gccgggatgt
     3961 gggcgccggc cggagttccc gctgacgtcg tggaaaagct caacgccgcc gtcaacgcgg
     4021 cgctgcagtc ctctgagacg atcgccagca tgaaaaagct cggcttcgag cttagaattg
     4081 gctcgtcgca ggatttcgcg gcttttgtcg cggcggaaat tccgcgctgg accacggtgg
     4141 tgaaagcgtc cggcgtgaaa tcacagtgag ttctcggccc gccattcaga acacttcaag
     4201 caaggtggga agtcgcccat gagcacgtcg aaaaaggtcg ttgtcgtcgg cgccggcatc
     4261 ggtggactga ccgccgcgct tgccttgctc aagcgcggta tcgatgttga agtttacgaa
     4321 cagtcggacc agctcaagga agtgggcgcc ggcatccaga tcagctccaa tggcactcgc
     4381 gtcttgtttg cgctcggcct ggaaacggcg ctcgccaacg tgcaggttcg gccggagcgc
     4441 cgcgagttgc ggcactggag caccggcgaa acctggaact ggttcgatct cggcgacaaa
     4501 agcatcgagc gattcggcac gccgcatctc atgctccacc gcgccgacct gcatggcctc
     4561 ctcgccgacg cggttcgctc gctgaaaccc cgcgccatca ggctgaacaa gcgatgcgtc
     4621 tcggtcgtct cccacgctgg acacgtcgaa gcgacgttcg aggatggcgg caccgtcaag
     4681 gcgccttatc tgatcggcgc cgatggtatt cattccaagg tccgcgtctg cctgttcggg
     4741 ccgagcaagc cgatattcac cgcctgcatc gcctggcgcg gcctgatccc gatggagaag
     4801 ctgccgcccc gtctcgctcg catggtcgga acgaactggc tcggtccgcg cggcaatgtg
     4861 ctgcactatc cggtgcgccg cggcgagatc atgaacttcg tgagcacgtc ggaacgcgac
     4921 gattggcagg tcgagtcctg gtccacggta gggaccaagg aagagctgcg caacgatttc
     4981 cgcgattggc acgccgacgt ccagg
//
LOCUS       DATLJT010000331         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1856222,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000331 DATLJT010000000
VERSION     DATLJT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856222"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VM910_07595"
     CDS             complement(<1..882)
                     /locus_tag="VM910_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVI62442.1"
                     /translation="MPIVNRVADLQPDIQAWRRSIHQYPELLYDVHRTAAFVADRLRE
                     FGCDEVATGLGKTGVVGVIKGKRGPGSGEIKVIGLRADMDALPIEEKTDLSYASKTPG
                     KMHACGHDGHTAMLLGAARYLAETRNFAGKAVVIFQPAEEGGGGAAAMIKDGLMDRFG
                     IDQVYGMHNGPGIPIGAFAIRPGPIMAATDSIDITIEGYGGHASRPHKCIDSVLVGSQ
                     LITALQQVVSRNVDPLDSAVISICEFHAGTARNVIPQIAELHGTVRTLTPEVRELVEK
                     RVREVVRGIAQITGAKID"
     gene            1016..2632
                     /locus_tag="VM910_07600"
     CDS             1016..2632
                     /locus_tag="VM910_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI62443.1"
                     /translation="MKRLEGDFDFIVVGAGTAGCIVANRLSADPNKRVLILEAGGNDN
                     WIWFHIPVGYLFAIGNPRSDWMFKTESEPGLNGRALAYPRGKVIGGCSAINAMISMRG
                     QAADYDHWRQLGLAGWAYDDVRPLFRRLEDHFLGESEHHGTGGGWRVEAPRLSWKILD
                     AVGDAAEEMGIRKIPDFNTGDNEGVSYFHVNQKRGRRWSSARGFLKPALTRQNLRLET
                     HVLVDRLIVENGRAVGVRFLQNGEVIEARASGEVLLCAGAIGSVQVLQRSGIGPAEWL
                     APLGIDVVLDKPGVGRNLQDHLQQRAIYRVTGVRTLNETYWSLPRRAMMGLDYAFRRR
                     GPLTMAPSQLGIFTRSDSTRARANIQFHVQPLSLDKFGDPLHRFPAITVSACNLQPTS
                     RGTVRIRSKEPDEAPSIAPNYLSTEEDRQVAADAIRTTRRLMAQKALARFSPKELLPG
                     PSVGDDDASLAKAAGDIGTTIFHPVGTAKMGTANDPLAVVDERLRFFGIGGLRVLDAS
                     VMPTITSGNTNTPTAMIGEKGAEMILQDAR"
BASE COUNT          475 a          888 c          850 g          497 t
ORIGIN      
        1 atcgattttg gcaccggtga tctgggcaat gcccctgacc acctcgcgta cgcgcttttc
       61 gacgagttcg cggacctcgg gcgtcaaggt gcgcacggtg ccatgcagct ccgcgatctg
      121 cgggatcacg ttgcgcgccg taccggcgtg aaattcgcag atcgagatga cggcggagtc
      181 cagcggatcg acattgcgcg atacgacctg ctgcagcgcc gtgatgagct gcgagccgac
      241 cagaacggaa tcgatgcatt tgtgcggccg cgacgcgtgt ccgccatagc cctcgatggt
      301 gatgtcgatc gaatccgtcg ccgccatgat cggaccgggc cggatcgcaa aggcgccgat
      361 cggaataccg ggaccgttgt gcatgccgta gacctgatcg atgccgaaac ggtccatcag
      421 gccgtccttg atcatcgcag cggcgcctcc cccgccctcc tccgcaggct ggaaaatcac
      481 gaccgccttg ccggcaaaat tcctggtctc ggcgagatag cgcgcggcgc cgagcagcat
      541 cgcggtgtgg ccgtcatggc cgcaggcgtg catcttgccg ggcgtcttcg aggcgtagga
      601 aagatcggtc ttctcctcga tcggcagcgc gtccatgtcg gcgcgcagtc cgattacctt
      661 gatttcgccc gaacccgggc cgcgcttgcc tttgatgacc ccgaccacac ccgtcttgcc
      721 aagcccggtc gcgacctcgt cgcagccgaa ttcgcgcagg cgatcggcga cgaatgctgc
      781 agtgcgatgc acgtcgtaca gcaattcggg atactggtgg atgctgcggc gccaggcctg
      841 gatatcgggt tgcaggtcgg cgacgcggtt cacgataggc atggtaaatc tcgttggttt
      901 ggccggctgc ttctctgtag cacggccgga acgcctcgcc catcacctcc gcgggacctg
      961 tacagcggcg acaggcggcg ctatggcgtt aaaaattccg aaaagtgagg acgttatgaa
     1021 gcgtcttgaa ggcgatttcg actttatcgt cgtcggcgct ggcaccgccg gctgcatcgt
     1081 cgccaaccgc ttatcggccg atcccaacaa gcgcgtcctg atcctagagg ccggcggcaa
     1141 cgacaactgg atctggtttc acatcccggt gggctacctg tttgcgatcg gcaatccgcg
     1201 ctcggactgg atgttcaaga ccgaaagcga acccggcctc aacggccgcg cgctcgccta
     1261 tccgcgcggc aaggtgatcg gcggctgctc ggccatcaac gcgatgatct cgatgcgagg
     1321 ccaggctgcc gactatgacc actggcgcca gcttggactt gccggttggg cctacgacga
     1381 cgtgcgcccg ttgtttcgcc gcctggaaga tcattttctc ggcgaaagcg agcaccatgg
     1441 cacaggcggc ggctggcgcg tcgaggcgcc acgtctgtcc tggaaaattc tcgatgcggt
     1501 cggcgatgcc gccgaagaga tgggcatcag gaagatccct gacttcaaca ccggcgacaa
     1561 tgagggcgtc agctatttcc acgtcaacca gaaacgcggg cgacgctggt cgagtgcgcg
     1621 cggttttctc aaaccggcgt tgacgcggca gaaccttcgc ctggaaactc atgtgctggt
     1681 cgatcgcctg atcgtcgaga acggccgcgc cgtcggcgtg cgcttccttc aaaacggcga
     1741 ggtcatcgag gcgcgcgcat caggcgaagt cctcctgtgc gccggcgcga tcggatcggt
     1801 gcaggtgctg cagcgctccg gcatcggccc tgccgagtgg ctggcaccgc tcgggatcga
     1861 tgtcgtgctc gacaagcccg gcgtggggcg caatctgcag gatcatctgc agcagcgcgc
     1921 gatctacagg gtgacgggag tccgcacgct gaacgaaacc tattggtcgc tgccgcggcg
     1981 ggcgatgatg gggctcgact atgctttccg gcgccgcggc cccctgacca tggcgccgtc
     2041 gcagctcggc atattcaccc gctccgattc cacgcgcgca cgcgccaaca tccagtttca
     2101 cgtacagccg ctgtcgctcg acaagtttgg cgatcccctg caccgttttc ccgccatcac
     2161 cgtgagcgcc tgcaatctgc agccgacctc gcgcggcacc gtgcgcatcc gctcgaaaga
     2221 accggacgag gcgccctcga tcgcgccaaa ttatctgtcc accgaagagg accgccaggt
     2281 tgccgccgac gcgatccgca ccacgcggcg gctgatggcg cagaaggcgc tggcgcgctt
     2341 ttctccaaaa gaattgctgc cgggcccgtc ggtcggcgac gacgatgcat cgctggcgaa
     2401 agccgccggc gacatcggta ccacgatctt ccatcccgtc ggcaccgcga aaatgggcac
     2461 ggcgaacgat cctctcgccg tggtcgacga gcgcttgcgc ttcttcggta tcggcgggct
     2521 tcgcgtactc gacgcttccg tcatgccgac catcacctcc ggcaatacca acacgccgac
     2581 cgcgatgatc ggcgagaagg gcgcggaaat gatccttcag gatgcacgtt aggccacgcc
     2641 cacggtttca cggtttcgcc ctttccttca cgggattgtc attgaccgcg ctcgctgggc
     2701 aggtgccccg
//
LOCUS       DATLJT010000332         4308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2316243,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000332 DATLJT010000000
VERSION     DATLJT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316243"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VM910_07605"
     CDS             complement(<1..1546)
                     /locus_tag="VM910_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVI62444.1"
                     /translation="MAARAGKLTESADNGAFAARLRREITGEVLFDRYSRGRYATDAS
                     FYQIMPHGIVVPRTMDEALRALAIARDDGRIVTARGGGTSQCGQTVNHGLIIDFSKYL
                     NRLLSLDIENRSCIVEPGIVLDDLNRQLKKHGLWFPVDVSTASRATIGGMAGNNSCGG
                     RSLRYGTMRDNTLSLDAVLADGTPLHFGEVPPDLSPANAQDGGLDLFRDMLALGAREA
                     DEISERFPKVQRRVGGYNLDALVPRNAPNNMAHLLVGSEGTLALTTGVELKLWPVIRN
                     KVLGVCHFGSFYQAMDAAQHLVKLRPIAVELVDRTMIALGRDIAMFEPVIAAAIKGDP
                     DAVLVVEFAEEDQDENLRRLRELSGLMGDLGFSWNNPQRTWGGVVDITEPALQTAIAD
                     FRAAGLNVMMSMKEQGKPVSFVEDCAVPLNHLADYTERLNAIFARHGTSGTFYAHASE
                     GCLHVRPVLNLRLEKDVHAMRAIAEEAFAMVREYKGSHSGEHGDGIVRSEFHEAMFGP
                     RIAEDFR"
     gene            1740..2948
                     /locus_tag="VM910_07610"
     CDS             1740..2948
                     /locus_tag="VM910_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HVI62445.1"
                     /translation="MTAPTGRHFLQIPGPSPVPDRILRAMDMPVIDHRSEEFARLART
                     VLEGSQKIFQTSGPVLIFPSSGTGAWEAAIVNTLSPGDKVLMVETGHFATMWRNMALR
                     WGIEVDFLPGDWRRGADPAEVETRLAADKSHAIKAVMVVHNETSTGATSRIAEIRATM
                     DRVGHPALLMVDTISSLGSVDYRHEEWKVDVSVSCSQKGFMLPPGLGFNAISEKARAA
                     SKTNKMPRSYWDWEEMLKPNAKGFFPYTPATNLLFGLREAIAMLLGEGLDKVFARHQR
                     LAAATRTAVDHWGLEVLCQEPLDYSPVLTAVLMPPGHDADQFRKIVLDNFNMSLGSGL
                     SKVAGKVFRIGHLGECNELTLLAALTGVEMGLSVAGIPHRSGGVDAAMSFLEQRKQSN
                     TAAHLKIVGS"
     gene            3097..4296
                     /locus_tag="VM910_07615"
     CDS             3097..4296
                     /locus_tag="VM910_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVI62446.1"
                     /translation="MTVHTGRHFLQIPGPTNIPDRVLRAMDMPGLDHRGPEFAELGFA
                     VLSGMQRMFRTEQPVIVYPSSGTGAWEAAIVNTLQPGDKVLMCETGQFAVLWLGIAQK
                     FKLDVDFVPGDWRHGADLGEIEARLAADKAHKIKAVCVVHNETSTGCVTSPLEVRKIM
                     DRTRHPALLMVDTISGLGSMEYEHDAWGADVSIAGSQKGLMLPPGLGFNAISEKARAV
                     AKANPAMRSYWEWEDVLAFNKAGTFPYTPAINLLMGLREAIKMLEEEGLENVFARHKR
                     HSAATRAAIKVWGLETQCQEQGAHSPALTGVRMPEGHDADHFRKVVLENFDMSLGTGL
                     NKIKGKVFRIGHIGHFNDLMLMGTLSGVEMGLDLAKVPHRSGGVVAAMDVLKGRDQTA
                     MPKAAVA"
BASE COUNT          813 a         1342 c         1382 g          771 t
ORIGIN      
        1 ccctgaaatc ttcggcgatg cgcgggccga acatcgcctc gtggaattcc gagcgcacga
       61 tgccgtcgcc atgctcgccg gaatgcgagc ctttgtactc gcgcaccatc gcaaacgctt
      121 cctccgcgat ggcacgcatc gcgtgcacgt ctttctccag gcgcaggttc agcaccggcc
      181 ggacatgcag gcagccttcg gaggcatgcg catagaaggt gccgctggtg ccgtgacgcg
      241 cgaagatcgc gttcagccgt tcggtatagt cggcaagatg gttgagcggc accgcacagt
      301 cctcgacgaa ggaaacgggc ttgccttgct ccttcatcga catcatcacg ttgaggccgg
      361 cggcgcggaa atcggcgatc gcggtctgca gcgcgggctc ggtgatgtcg accacgccgc
      421 cccatgtgcg ttgcgggttg ttccaggaaa agccgagatc gcccatcagc ccggaaagct
      481 ctctcaggcg gcgcaggttc tcgtcctggt cctcttcggc gaattcgacg accagcaccg
      541 catcggggtc acccttgatc gccgccgcga tcacaggctc gaacatggcg atgtcgcgtc
      601 cgagcgcgat catggtgcgg tcgaccagtt cgaccgcaat gggacgcagc ttcaccagat
      661 gctgggcggc atccatcgcc tggtaaaagc tgccgaagtg gcagacgccc aggaccttgt
      721 tgcggatgac gggccacagc ttgagctcga ccccggttgt aagcgcgagc gtgccttccg
      781 agccgaccag aagatgcgcc atgttgtttg gcgcattgcg gggaaccagc gcgtcgagat
      841 tgtagccgcc gacccggcgc tgcactttcg ggaatcgctc cgagatctcg tcagcctcgc
      901 gcgcgccgag cgcgagcatg tcgcggaaca ggtccaaccc gccgtcctgt gcattcgccg
      961 gcgaaagatc cggcggcacc tcgccgaaat gcagcggcgt gccgtcggcc aaaaccgcat
     1021 ccagcgacag cgtattgtcg cgcatggtgc cgtagcgcag cgagcggccg ccgcaggaat
     1081 tgttgccggc catgccgccg atggtggcgc gcgaggcggt cgagacgtcg accggaaacc
     1141 acaagccgtg cttcttgagt tgacggttca aatcgtcgag cacgatgccc ggctcgacga
     1201 tgcaggagcg gttctcgatg tcgagagaca ggagccggtt caggtatttt gagaaatcga
     1261 tgatgaggcc gtgattgacg gtctggccgc attgcgaggt gccgccgccg cgtgcggtga
     1321 cgatccggcc atcgtcgcgg gcaatcgcca gcgcccgcag cgcctcgtcc attgtgcggg
     1381 gcaccacgat gccgtgcggc atgatctggt agaaggaggc atcggtcgcg tagcggccgc
     1441 gactgtaccg atcgaacagc acctcgccgg tgatttcgcg ccgcagccgc gccgcaaacg
     1501 caccgttgtc agccgattct gtcaacttcc cggcgcgcgc cgccatcgtg tccagtccgt
     1561 cattaagcct ttgcatctct tgcccgccgg ggttccgatt ggcaaggtga agtccggcct
     1621 tctgatgcat ttggcgacgt ctgcccagca ggtgctgcgg gggacagacg cgcaaaatag
     1681 tgatatccga acagcttgcg atagcttcac catccaaaac tgttgccagg acgcttgcca
     1741 tgaccgcgcc caccggaagg cactttctgc aaattccggg acctagcccg gttcccgacc
     1801 gcatcctacg cgcgatggat atgccggtca tcgatcatcg cagcgaggag tttgcgaggc
     1861 tggcgcgaac cgtgctcgag ggcagccaaa aaatcttcca aacctccggc cccgtcttga
     1921 ttttcccctc ctcggggacc ggcgcctggg aggccgcgat cgtcaacacc ctgtcgcccg
     1981 gtgacaaggt gttgatggtg gagaccggtc acttcgcgac catgtggcgg aacatggcct
     2041 tgcgctgggg catcgaggtc gactttctgc cgggcgactg gcggcgcggc gccgatccgg
     2101 ccgaggttga aaccaggctc gccgcggaca aatcgcatgc gatcaaggcc gtgatggtcg
     2161 tgcacaacga gacctcgacg ggtgcgacca gccgcatcgc cgagatccgt gccacgatgg
     2221 accgcgtggg ccatccggcg ctattgatgg tcgacaccat ctcctcgctc ggctcggtcg
     2281 attaccggca cgaggaatgg aaggtcgatg tcagcgtgag ctgctcgcaa aagggattca
     2341 tgctgccgcc aggcctcggc ttcaacgcga tctcggaaaa ggcgcgcgcc gcctcgaaga
     2401 ccaacaagat gccgcgttca tattgggact gggaggagat gctgaagccg aacgccaagg
     2461 gcttctttcc ctatacgccg gcgaccaacc tgctctttgg cctgcgcgag gccatcgcga
     2521 tgctgttggg cgaagggctc gacaaggtct ttgcccgcca ccagcggctt gcggcagcaa
     2581 cccgcacggc cgtcgatcat tggggcctgg aagtgctctg tcaggagccg ctcgactatt
     2641 cgccggtgct gacagcggta ctgatgccgc cggggcacga tgccgaccag ttccggaaaa
     2701 tcgtgctcga caatttcaac atgtcgctcg gaagcgggtt gtccaaggtc gccggaaagg
     2761 tgtttcgcat cggccatctc ggcgagtgca atgagctgac gctgctcgcg gcgctcacgg
     2821 gcgtggagat ggggctttcg gttgccggca tcccgcatcg ttcgggcggc gtcgatgccg
     2881 ccatgagttt tttggagcag cgcaagcaaa gcaataccgc agcgcacctc aagatcgtcg
     2941 gcagctagca acggccggcc gcaaacgccg aatcgggtaa aaatccagct tgttatgcat
     3001 tgacgggcga aagccggcga gcgacaagcc ggacgaacaa tgttatccga cccgccaagc
     3061 tgccgctaaa gccataagag accgggaaga acgctcatga ccgtgcacac tggaagacat
     3121 tttctccaga ttccagggcc aaccaacatc cccgaccggg tgttacgggc catggacatg
     3181 ccgggtctcg atcaccgcgg tcctgaattt gccgagcttg ggtttgcggt gctttcgggc
     3241 atgcaacgga tgttccgtac cgagcagccg gtgatcgtct atccctcctc cgggacaggt
     3301 gcgtgggaag ccgccatcgt caacacgctg cagccgggcg ataaagtgtt gatgtgcgag
     3361 acaggacagt ttgccgtgct gtggctcggc attgcccaga aattcaagct tgatgtcgac
     3421 ttcgttcccg gcgactggcg ccatggcgcc gatctcggcg aaatcgaggc ccgccttgcg
     3481 gccgacaaag cacacaagat caaagcggtg tgcgtggttc acaacgagac ctcaaccggc
     3541 tgcgtcacct caccgctcga ggtgcgcaag atcatggacc ggacgaggca tccggcgctc
     3601 ctgatggtcg ataccatctc cggtctcggc tcgatggaat atgaacatga tgcctggggc
     3661 gccgatgtct cgattgccgg ctcccagaag ggcctcatgc tcccgccggg cctcggcttc
     3721 aatgcgatct cggagaaggc ccgcgcggtg gccaaggcca atccggcgat gcgatcctac
     3781 tgggagtggg aggacgtgct cgccttcaac aaggcaggta ccttccccta tacgcccgcc
     3841 atcaacctgc tgatgggcct gcgcgaagcc atcaagatgc tcgaggaaga ggggctcgag
     3901 aacgtgtttg cccgccacaa gcgccatagc gccgcgacgc gcgcggcaat caaggtgtgg
     3961 ggtctggaga cgcagtgcca ggagcagggc gcgcattcgc cggcgctgac cggcgtgcgc
     4021 atgccggaag ggcacgacgc cgaccacttc cgcaaggtgg tgctggagaa tttcgacatg
     4081 tcgctcggca ccgggctgaa caagatcaag ggcaaggtat tccgcattgg ccatatcggc
     4141 catttcaacg acctgatgct gatgggtacg ctgtccggcg tcgagatggg tctcgatctt
     4201 gccaaggtgc cgcaccgctc cggcggcgtg gtggccgcga tggacgtgct caagggccgc
     4261 gaccagactg cgatgccgaa ggccgcggtg gcctgaagcc ctagacga
//
LOCUS       DATLJT010000333         8055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3235586,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000333 DATLJT010000000
VERSION     DATLJT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235586"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..1093)
                     /locus_tag="VM910_07620"
     CDS             complement(515..1093)
                     /locus_tag="VM910_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62447.1"
                     /translation="MDLQSSPSIATLRTALDVPEGTLIGNHHRVLIQRTRGLIHTVKF
                     VSKPRPGTCVAFAFGLSNDPVYKTIAQDFGRKVFAGKRFVEWMIRHRLQEIDPAKIGS
                     LVLYFSGSDWRHIGTETGLHQVTSQWGTYPIYEHGRCEVPERYGDVVRFFDKPPPGQA
                     LADFLDYARSEGVSNEAIKAIIAEEARPLRRK"
     gene            complement(1699..3786)
                     /locus_tag="VM910_07625"
     CDS             complement(1699..3786)
                     /locus_tag="VM910_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVI62448.1"
                     /translation="MASAPDALHQNRLSRETSPYLLQHQHNPVHWWPWGPDALAEAKR
                     TNKPILLSVGYAACHWCHVMAHESFEDEATAAAMNELFVSIKVDREERPDIDQIYMNA
                     LHLLGEQGGWPLTMFLAPDGSPFWGGTYFPKTSQYGRVAFTDVLREVARIFRDEPNKI
                     AQNRNSLVAKLAERARSDNPANIGITELDSAATSIARATDPVNGGLRGAPKFPQCAML
                     EFLWRAGARTKDDRFFLTTELALTRMSQGGIYDHLGGGYARYSVDDKWLVPHFEKMLY
                     DNAQILDTLALDYARIKNPLFRERAAETVGWLRREMTTPEGGFASSLDADSEGEEGKF
                     YVWSLEEIEHALGPADAAPYAAGDSAAFFAAKYNLSRNGNFENSNILNRLNGLADTSD
                     EAGRLAMLRSMLQQERTKRVRPGLDDKVLADWNGLMIAALAHSAAAFDAPDWIEPART
                     AFDFVARSMTQDDRLGHSWRAGRLLIPGLASDYAAMIRAALAIFEASGEQSYLDCALT
                     WQHSLDAHYADADHGGYYLTANDAEGLIVRPHSTVDDAIPNHTGLIAQNLVRLAVLTG
                     DDNCRSKADALFTALLPSAAENVFGHLSLLNALDLHLSGAEIVVVGEGARTDALLATA
                     RKLPHGTSIVLHAPRADVLSTTHPARAKLYATTDGAAFVCRGQSCSLPVTESDVLVQM
                     VASPAAPVERLNL"
     gene            3908..4729
                     /locus_tag="VM910_07630"
                     /pseudo
     CDS             3908..4729
                     /locus_tag="VM910_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            complement(5471..7711)
                     /locus_tag="VM910_07635"
     CDS             complement(5471..7711)
                     /locus_tag="VM910_07635"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HVI62449.1"
                     /translation="MADQIVITEKTSQAKDVRAAVGSRYGDILPAEGHLFDLVEPEDV
                     VPAWKRWSPILLRPEGLYGTRPAEGGNKAAKLRAIREALRTAKRVWLATDCDREGQLI
                     GQEILEHYNYRGQVMRVLFTAQDSQTIRDAFGRAKPNAEYSRLYAAAVARRQADQIYN
                     LSLTRTATVILGQGARRVIGVGRVKTPTLAIVCRRELEIRNFVPLAYFEVVATAKVAG
                     GQFQMRHAPQDRIVKREIAQDVVKVADGFEGALTVRVEDKRQGPPKLHDLPSLQKLCS
                     SRFGWPAGKTLEVAQELYDGQGKKVITYPRAEVRYLPQSLISDVPRIVAGLRVGQAFS
                     AIPVPDPPVIRRGASGAFYDKGLEGASHHAVIPNINTIDKLREIWPRLSSDEKKLFDV
                     IARAYLSALMPDFRYRQTSATIDVRGFEFRAVGRQPIDLGWRAAFPDWQPADKKGDEA
                     QLLPPLRNGETAKLQDPKIEDKETRPPPRYNEGTLIEAMQNAWRFVDDEILRERLKEA
                     KGIGTPATRAEIIGGLKKQDFLIAQGKNVLPTETGLSLFGVLKQADPALVDPGVTAEL
                     ECLLDDVVVGKQEMVGAIDAVCDVAQRIIGKLTEGATAGGPVLLGVSVGNSASEYPPT
                     PAMKRFADGLSRQKGIKPPPGYKTSISICRKFLSEHAPKKANGETAGKLDPKPVSPAQ
                     LLYAKKIAQGKGLIIPDEAKTSSATMSAWIKSNRGKMRRKRVAKTTYQPKRSAEPQST
                     VPAKRT"
BASE COUNT         1654 a         2369 c         2308 g         1724 t
ORIGIN      
        1 gaacattttg gtgattggtt aggcagcgcc gagtttcttc gatcttcgtc tcggttgccc
       61 cttctagtcc acccctccag cacgcctttg gccttgttta tctacgaacg attcctcgag
      121 gagctgcgaa cctctaggag ctggatcaat gcacctagcg cgaacccact gatgaccagg
      181 gccatgagga tttcctgtgg ctctaaattc gcaccgcaca ttagtcccgc tacgaagagt
      241 agccatgagt ataaaaatag gaacacgagg cgcggtttcc tcgacgtaga tccgcgcgtg
      301 gctgtgtccc tgatagttgt cggcatcctc gtcccccctc aaggtaccta aaccttactc
      361 gtctcatcaa acttcgacga tttcggggtc cagatgtccc cgttattctc aagcgcccca
      421 ttggccagaa atacgcaggg ctgtgggagg atgagcccac aatcgcgggc aaacgcgcct
      481 tgacacgacg aagaaatgag tgactgagcc gccgctactt ccttcggagg ggacgcgctt
      541 cttcggcaat aattgccttg atagcttcat tcgaaacacc ctcgctgcgg gcatagtcca
      601 agaaatcagc aagcgcttgc ccgggcggtg gcttgtcgaa gaaacgtacg acgtcgccgt
      661 aacgttcagg cacctcacac cgtccgtgtt catagatcgg ataggtcccc cactgagagg
      721 tgacctgatg caatccagtt tcagtcccga tgtgtctcca gtcggagcct gaaaagtaca
      781 gaacgagcga cccgattttg gcgggatcga tctcctgtag acgatggcgg atcatccatt
      841 cgacaaatcg ttttcccgcg aaaaccttgc gaccgaagtc ctgtgcaata gttttgtaga
      901 ctggatcgtt gctcagcccg aaagcgaacg cgacgcaagt acccggccgc ggcttggaca
      961 cgaatttaac agtgtgtatc agtcctcgcg tccgctgaat tagcacgcgg tgatggttgc
     1021 caatgagagt cccttctggg acatctaaag cagttcgcag ggtggctatg cttgggctgc
     1081 tctgcaggtc catgaagctt ttcctcctgc aaggctgcta tctcgcgcga accgcgttcc
     1141 tcatcacgag gtaatttttc ggcgaggctc cttatcacga ggtaattctt cggtaatcag
     1201 ttcgactggg gactgggcaa atcagtcgtc tgccgctacg gctaggagat gcaacgtcct
     1261 agccctcatg tagccaacgc ctcccgctcc cgcttcgccc gctccagggt tcacgaacgg
     1321 acgactaccc gctggcatag atggccgcag tcccgccgct agcgcgatct ggtgcgcgcc
     1381 acgagttttt ccaagatcga gaaccagatt ttcacagcca ggagaccagc gtcggcgtga
     1441 agtggaaaac gcaggttcag atgacgtgag ccagcagtca ttcgcgggct tgggggtatc
     1501 tctgacctga aagcgaagcc aagacgccaa gaatcagctt gagcagatca gtcatcaggc
     1561 cgcgagtaac tcgtccgttc cctgcgtcta tgacgaatcg aattttcggt acacccgggt
     1621 ctgggaccct accgccctta ccaaaaagat gtcgtccgaa agtcttcagt catccgtcga
     1681 accgcaaagg ccagccgatc agagattgag tcgttcgacc ggggccgcgg gcgacgcgac
     1741 catttgcaca agtacatccg attctgtcac cggcaacgaa cagctttgtc cgcgacagac
     1801 aaacgccgcg ccatctgttg tggcatagag ctttgcacgc gcgggatgag tggttgaaag
     1861 cacatcagcc ctcggcgcat gcaacacgat agatgtccca tggggaagtt ttcgcgcggt
     1921 cgcaagaagt gcgtcagtac gcgcgccctc tccgaccact acgatttccg caccgctcaa
     1981 atgcagatcg agcgcgttga gcaacgacag atgtccgaac acattttcag cggcgctcgg
     2041 cagtagcgcg gtgaacagcg catcggcctt gctgcgacaa ttgtcgtcgc cagtgagcac
     2101 cgcgagacgc acaagatttt gagcaatcag acctgtgtga ttcggaatcg catcatcgac
     2161 ggtcgaatgc ggccggacga tcaatccttc cgcgtcgttc gccgtgagat aatagccgcc
     2221 atggtcggca tcggcgtaat gcgcatccag cgaatgctgc caggtcagcg cgcagtcgag
     2281 gtaagactgc tcgccgctgg cctcgaatat tgcgagggcc gcccggatca tcgcagcata
     2341 gtcggaggcc agtccgggta tcagaagccg tccggcgcgc caggaatgtc ctagacgatc
     2401 gtcttgggtc atggaccgcg cgacgaagtc gaaggcggta cgggccggtt caatccaatc
     2461 cggagcatcg aaggccgccg cgctgtgggc aagggctgca atcatcagcc cgttccagtc
     2521 ggccaagacc ttgtcgtcga gcccgggccg cacccgtttg gtgcgctcct gctgcagcat
     2581 cgaccgcagc atggcgaggc ggcccgcttc atcggaggtg tccgccaagc cattgaggcg
     2641 attgaggata ttgctgtttt caaagttccc gttgcgggag agattgtatt ttgcagcaaa
     2701 aaatgctgcg gaatcgccag cagcataagg cgcagcatcg gcgggaccga gcgcgtgttc
     2761 gatctcttcg agcgaccaga cgtagaactt gccttcctcg ccttccgaat cggcatcgag
     2821 cgacgacgcg aatccgccct cgggcgtggt catctctcgt cggagccagc cgaccgtctc
     2881 ggccgcgcgt tcgcgaaaca gcggattttt gattcgcgca taatcgagcg ctagcgtgtc
     2941 gaggatctgc gcgttgtcgt agagcatctt ctcgaaatgc ggcaccaacc atttgtcgtc
     3001 gacggaatag cgcgcgtaac cgccgccaag atgatcgtag atgccgcctt ggctcatgcg
     3061 cgtcagcgca agctcggtgg tgagaaaaaa ccgatcgtct ttggtgcgtg caccggcgcg
     3121 ccacaagaat tcaagcatcg cgcattgcgg aaatttgggc gcgccgcgca agcccccatt
     3181 gaccggatcg gtcgcacgcg caatggatgt cgccgcactg tcgagctctg ttatgccgat
     3241 atttgctgga ttatccgaac gcgctcgttc ggcgagcttc gcaacaagcg aattccggtt
     3301 ctgtgcgatc ttgttaggtt catcgcgaaa gatgcgcgcg acttcgcgca atacgtcggt
     3361 aaatgcgacg cggccgtatt gcgatgtctt cggaaaatat gtgccgcccc agaacggcga
     3421 gccatcgggc gcgagaaaca tcgtcagtgg ccagccgccc tgctcgccta acagatgcaa
     3481 tgcgttcatg tagatctggt cgatgtcggg tcgttcctcg cgatcgacct tgattgagac
     3541 gaacaactcg ttcatcgccg cagcggtggc ttcatcctca aagctctcat gcgccatgac
     3601 gtggcaccaa tggcacgcgg cataaccgac cgagagcaga atcggtttgt tggttcgctt
     3661 cgcctccgca agcgcgtcgg gtccccacgg ccaccagtgg accggattgt gctgatgctg
     3721 cagcagataa ggcgatgtct cgcgggagag gcggttttgg tgaagagcgt ccggtgccga
     3781 ggccatccat aaccgtacca caaacagctg cgcgccggta tcggtgaatt tcacgtaggt
     3841 gcggccttcg tgcagccaca gccagccctt ggcaatcgcc agaatcgcga gagctggatt
     3901 gcgtgccatg gcacaagagc ggccagtccg ggtcgagcgc agattgtcgg cgataatggc
     3961 tgccgacgtg gctggctact cgcggctcat gcacaatgac gaagaggcta cgcatgccaa
     4021 actgacaacg ctcctgacgg aggctgtcgc cccggcaatt gccgaacacg gcggccgcat
     4081 tgtcaagaac actggggacg ggttcttggc ggagtttccg agcgcggtcg aggcggttcg
     4141 agccgctatg cagttccaga cccgcatcaa cgaccttaca atcggtgatg tggaagagcg
     4201 cattgctttc cgtgtgggca tcaatatcgg tgacgtcatt gtcgagccgc atgacatttt
     4261 tggggatggg gtgaacatcg cggcgcgact tgagagcatc gcagaacccg gcggcatatg
     4321 catctcgtct tccgcctacg atcatgtccg aggcaaggtc ggggttgagt tcgacgatct
     4381 cggcgaacga gcgttgaaaa atatcgaccg gccggtgcgg ctctatatgg cgcggatcgc
     4441 cacagcgccg ttagctgaac gcgccaactc atctgccgaa agccaaaagc cgctgcccct
     4501 ccccgacaag ccgtccatcg ccgtcctgcc cttccagaac atgagcggtg atcctgagca
     4561 ggaatatttt gccgatggaa tggtcgaaga aatcattacg gccctttctc gaaacaagca
     4621 gcttttcgtc atcgcccgga actccagttt cacgttcaag ggcaaagctg tcgacatcaa
     4681 gcaggtggct cgggatttag gggtccgata cgttcttgag gggagtgtcg cttgagcagt
     4741 tcgccgtgga tacgtggcgc accccaaagc tggttctccg tgctcatctg caggatcagc
     4801 gcgcgcaatt ctatctcgat ttgcgggcgc cctccccgcg agttcgattt ccaacgccaa
     4861 tagcgacgaa agccggcccg atgccaccgc accagcgttt caggttgaac aatcgtaacg
     4921 accttcaaga tcgatggaaa ccatcgatac atctggacga ggaaccagcg gtcgttgttg
     4981 gtaggctgag ccgacccctc accttgcgcc gcaagacgat cagctgatgt cggagcaccg
     5041 cattctccgc ctcaagcctg ctcttcgact tgaacggcga ggccaacacg gccagaacaa
     5101 aacagataag cgcgatcatc gcgtcaactt cttcgctcaa caggccccga tacggttttc
     5161 ggtacacaca ggacgcgctg ctactgttct tagattggtt ttcgaaaaat gaacattgtc
     5221 tgacgccgat gagtttcccc gcgccgatta ttatttatgg aaagcgaact tgtttcggcg
     5281 atatgccgcc ttttttttag ggcaggcttg cacacccgtt gaagagtcaa ctgcctcaga
     5341 ccgatcgcca ggcacgaata agaaatggga ctcgaacggg ttatgaggta ttgctttgca
     5401 agtcctttgc cgattttgat tttggctgag cgcgagcccg cggcgcagcg gagggttcgc
     5461 ctttgcgctt tcaagtcctc ttcgcaggca cggtcgattg aggttcagct gacctcttcg
     5521 gctggtaagt ggtcttggca acacgcttgc ggcgcatctt gcctcggttc gacttgatcc
     5581 acgcagacat cgtggccgag ctggtcttcg cctcatcggg gatgatgagg cctttcccct
     5641 gcgcgatctt ttttgcgtac aataactgtg ctggactcac tggcttaggg tcgagttttc
     5701 cggccgtttc accattggct ttcttgggcg catgttcgct gagaaacttg cggcaaattg
     5761 atatcgaagt cttgtagccg ggcggtggct taataccctt ctgccggcta aggccgtcgg
     5821 cgaagcgctt cattgcaggc gttggcggat actctgatgc gctgttgccg actgacacgc
     5881 caagcaacac gggtccgccg gctgtggcac cttcggtgag cttgccgata atgcgttgcg
     5941 caacatcgca cacggcgtca atcgcgccga ccatctcttg tttgccgacg acgacgtcat
     6001 cgagcaggca ttcaagttcc gccgtcactc ccggatcgac gagtgccgga tcggcctgtt
     6061 tgagaacgcc gaacagcgaa aggccggtct cggtcggcag gacattcttt ccctgggcaa
     6121 tcagaaaatc ctgcttcttc agcccgccga taatttcggc tcgcgttgcc ggtgtgccaa
     6181 tacccttggc ttcctttagg cgttcccgaa gaatctcatc gtcaacaaaa cgccaagcgt
     6241 tttgcatggc ctcgatcaga gtgccttcgt tgtaacgcgg cggtggccgg gtctccttgt
     6301 cctcaatttt ggggtcctgc agcttcgcgg tctcgccgtt gcgcagtggc ggcagcaatt
     6361 gcgcctcatc gccctttttg tcggctggct gccagtccgg gaaggctgct cgccaaccga
     6421 gatcgatggg ctgacggccg acggcgcgga attcgaagcc gcgcacatcg atcgttgcgc
     6481 tcgtctgccg gtatcggaaa tcaggcatca gcgccgacag ataggcccgt gcgataacgt
     6541 cgaacagttt cttctcatcg gacgacagac gaggccagat ctcccgcagt ttgtcgatcg
     6601 tgttgatgtt gggaatgaca gcatgatggc tcgcaccctc cagccccttg tcgtagaagg
     6661 caccgctcgc gcctcttcgg atcactggcg ggtcgggcac ggggatggcg ctgaacgctt
     6721 ggcctacccg cagtccagcc acgatccgcg gtacgtccga aatcaagctc tgtggcagat
     6781 agcgcacctc ggcgcgagga taggtgatga ccttcttgcc ctggccatca tagagctcct
     6841 gcgccacctc gagcgtttta ccggccggcc agccgaagcg ggagctacac agtttctgca
     6901 acgaaggcaa atcatgcagc ttcggcggcc cttgccgctt atcctcgacg cgcacagtga
     6961 gcgcgccctc gaagccgtca gctactttga cgacgtcctg agcaatctcg cgcttaacaa
     7021 ttcggtcttg cggcgcgtgc cgcatctgga attgccctcc cgcaaccttc gcggtggcga
     7081 caacttcaaa ataggcgagc ggcacgaagt tccggatttc caactcacgc ctgcacacaa
     7141 tggccaaggt cggcgtcttc acccggccga cgccgatcac tctccgcgcg ccctggccca
     7201 ggataacggt cgcagttcgg gtcagtgaca gattgtagat ctggtcagcc tgtcgccgcg
     7261 caacagcggc ggcatagagg cgcgaatatt cggcatttgg ctttgcccgg ccgaatgcat
     7321 cgcggatggt ttgcgagtcc tgcgcggtga acagcacccg catgacttgg ccgcggtagt
     7381 tgtaatgctc gagaatctcc tgaccgatca gctgaccctc gcggtcgcag tcggtcgcaa
     7441 gccaaaccct cttggcggta cgcaacgcct cgcgaatagc tcggagcttg gcggccttgt
     7501 tgccgccctc tgccggccga gtgccgtaaa gtccttctgg ccgcagaagt attggcgacc
     7561 agcgcttcca ggccggcaca acatcctctg gttcgacaag gtcgaacaaa tggccctcgg
     7621 ccgggaggat atcgccgtag cgagaaccaa cagcggcgcg gacgtctttt gcctggctgg
     7681 ttttctccgt tatgacgatc tgatctgcca tgacggtcca ttggaagaat ggattcagtg
     7741 gaatctcgaa aagaacatat agtgaacctt attgattcac aagctggtct ttgccgcggc
     7801 ccactggctt gtaggctctc aggaccaaga aggtatgaag gtcggctttg gctacgactt
     7861 gctcaacggc aaccccccgt ctccgctgca atgcaaggta ccgtctctcc tatcccacaa
     7921 gcgcaaggtc agctggttac ttccagtttt tgagaatcga cgatctgaag acactgcatg
     7981 aaaccattgg agtcaacgtg gatgccggcg gaagctattt cagcgctagc gccgacgtaa
     8041 aagcgcagta cgcaa
//
LOCUS       DATLJT010000334         3797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_248509,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000334 DATLJT010000000
VERSION     DATLJT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248509"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VM910_07640"
     CDS             complement(<1..1234)
                     /locus_tag="VM910_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated channel"
                     /protein_id="HVI62450.1"
                     /translation="MYRFRFPRKRRRLLSASSLVLLAASGAPVLAQQADTTPPPTTGA
                     ATQPSASPRAESSPTPQDATPAPTAEQQGAGNVLPTTRVAAPAQKPRTPKPPTTSAVT
                     NPSPAPTEQQVVAQQNEKFDAARQNIFPSIGAGSYEVSHQAIEALPQGNNAPLDKALL
                     QFPGVTQDSAASGELHVRNEHANLQYRINGVMLPDGVGAFGQILDTGIVGTMALLTGA
                     LPAQYGLRTAGVLDIQTKADAFDNSGKISVYGGSHQNITPFGEYGGTVGQTQYYVSGR
                     YFYSNLGIENPTPANEAIHDRTQQEKGFLYLSTVLDPQSRLTLISGQSNALFQIPNNP
                     GQPTNFMTATQSTFNSALLNERQQEINSFNVLAYQHSAEGVDTQLSFFNRYSQLHFLP
                     DPIGDLLINGVASNVYRQS"
     gene            complement(1355..1717)
                     /locus_tag="VM910_07645"
     CDS             complement(1355..1717)
                     /locus_tag="VM910_07645"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVI62451.1"
                     /translation="MRLVRDHIGFGSWLALIALALNLALSFGHIHAIGGGQRNGLVAA
                     VAPSDGGQTGRHGDGLADDRCPICMASSSIASVLASAPPALAHPIAYILVDRTAPTAI
                     AFLESSRAAFQSRAPPLS"
     gene            complement(1795..2514)
                     /locus_tag="VM910_07650"
     CDS             complement(1795..2514)
                     /locus_tag="VM910_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62452.1"
                     /translation="MADARPLLEVNAIETCYGLSQVLFGLSLAIRAGEMVSLMGRNGM
                     GKTTTIRSIMGLTPARAGNIRFAGTEVSSLPSYRIAKLGIGLVPEGRQIFPNLTVREN
                     LIAASANRLGVSDPWTIEKIHALFPRLAERGGNMGNQLSGGEQQMLAIGRALMTNPHL
                     LILDEATEGLAPLIRQEIWNCLSMLKARGQSILVIDKNVENLTRICDRHYIIERGRTV
                     WSGSSEQLVAEPDLQHRYLGI"
     gene            complement(2507..3274)
                     /locus_tag="VM910_07655"
     CDS             complement(2507..3274)
                     /locus_tag="VM910_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62453.1"
                     /translation="MAEPLLRVQKLVRRFGGIIATDHVSLEIPQGELHAIIGPNGAGK
                     TTLISQLTGHLMPHAGSIFLGGRDITRLAAYRRSGLGLARSFQITSLLTDFTAADNVA
                     LAAQARDGHSFRFLRNARKEEGLRRTALAALQRVGLADRAEMLVSRLSHGEQRQLELA
                     VALATSPQLLLLDEPMAGLGPTESARMVKLLQDLRKEVTIILVEHDMEAVFALADRIS
                     VLVYGRVIAAGAPSDIRRNEEVKRAYLGDQHVVVRHG"
     gene            complement(3267..>3797)
                     /locus_tag="VM910_07660"
     CDS             complement(3267..>3797)
                     /locus_tag="VM910_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62454.1"
                     /translation="GDDGLTIYKRSTFAGLINLSNRVQFYYLCLACLLGVIYLIWRIV
                     NSRFGLVVQGLRSNEQRMQAIGFPAKRYRLVCFVIAGTMCGLAGALLANNTDFVSPAV
                     MYWTRSGDLMVMVILGGMGSLFGPAIGAVVYLVLEELLSQVTEYWALIMGPLLLMIVL
                     FGRGGIMGLLGRFDRG"
BASE COUNT          701 a         1159 c         1274 g          663 t
ORIGIN      
        1 cgctctggcg atagacgttc gacgcaacgc cgttgatcag caggtcgccg atcggatcgg
       61 gcaggaagtg tagctggctg tagcggttga aaaaggagag ctgggtgtcg accccttcgg
      121 ccgaatgctg gtaggcgagc acgttgaaac tgttgatctc ctgctgccgc tcgttgagga
      181 gcgcagaatt gaaggtcgac tgcgtcgcgg tcatgaagtt cgtcggctgc cccggattgt
      241 tggggatctg aaacagcgcg ttcgactggc cgctgatcag ggtcaggcgg ctctgcggat
      301 cgagcactgt cgagagataa aggaagccct tttcctgttg cgtgcggtcg tggatggcct
      361 cgttggcggg cgtcggattc tcgatgccga gattgctgta gaaatagcgg cccgacacgt
      421 aatattgcgt ttgccccacg gtgccgccgt attcgccgaa cggcgtgatg ttctggtggc
      481 tgccgccata gacgctgatc ttgccggaat tgtcgaacgc atccgctttg gtctggatgt
      541 cgagcacacc ggcggtgcgc aggccgtatt gcgcaggcag cgcgccggtc agcagcgcca
      601 tggtgccgac gatgccggtg tcgaggattt gcccgaaagc gccgacgccg tcgggaagca
      661 tgacgccgtt gatgcggtac tgcagattgg cgtgctcgtt gcgaacatgc agttcgccgc
      721 tcgccgccga atcctgcgtc acccccggga actgcagcag cgccttgtcg agcggcgcat
      781 tgttgccctg cggcagcgcc tcgatcgcct ggtggctcac ctcgtaggac ccggcgccga
      841 tcgacggaaa aatattctgt cgtgccgcgt cgaatttttc attctgttgc gccacgactt
      901 gctgctccgt tggcgcaggt gacggattgg tcacggccga ggtcgtcggc ggcttgggcg
      961 tcctgggctt ctgcgccggc gcggcgacgc gcgtggtggg gagcacgttg ccagcgccct
     1021 gctgctccgc ggttggtgca ggcgtggcgt cctggggcgt tggtgacgac tcggccctcg
     1081 gagaggcgga tggttgcgtg gccgcaccgg tggttggcgg cggcgtggta tcggcttgct
     1141 gcgccagcac cggagcgccg gacgccgcaa gcagaacaag cgacgatgcg gacagcagcc
     1201 gccggcgctt gcgcgggaat cgaaaacggt acatttgcgg aattcctgac gtcctgcatt
     1261 cacggcgacg cgaacacgtg atcaaacgaa gctccgcccc gctgttcgca gcgtggctca
     1321 gcacgcagtg ctcgagcggg agcgacttgc gacgtcagga aagcggcggc gcgcgcgact
     1381 ggaacgcggc ccgcgaggat tcaaggaagg caatggccgt cggagcggtg cggtcgacca
     1441 ggatataggc aatcggatga gccagcgctg gcggcgctga agccagcacg ctggcgattg
     1501 agctcgacgc catgcagatc ggacagcgat cgtcggcgag gccgtcgcca tggcggccgg
     1561 tctgaccgcc atcggaaggc gcgaccgcag cgacgagccc gttgcgctga ccgccgccga
     1621 tggcgtggat gtgaccaaaa gacagcgcaa ggttcagcgc cagcgcgatc agcgcgagcc
     1681 acgaaccaaa tccgatatgg tcacgaacca gacgcatccg atgcaaaccg tcaaacgatc
     1741 ttacccggcc agtcggtcgg gtcaatgaaa agtccggaag ggtttggtct gactttagat
     1801 accgagatag cggtgctgca agtccggttc cgcgaccagt tgctccgaac tgccgctcca
     1861 caccgtgcgg ccacgttcga taatgtaatg ccgatcgcag atgcgggtga gattttcgac
     1921 gttcttgtcg atcaccagga tcgactgccc gcgcgccttc agcatcgaca ggcaattcca
     1981 gatttcctgc cggatcagcg gcgccagccc ctcggtcgcc tcatccagga tcaacagatg
     2041 cgggttggtc atcagggcgc ggccgatcgc cagcatttgc tgctcgccgc cggagagctg
     2101 gttgcccata ttgccgccgc gttccgcaag gcgcgggaac aaagcgtgga tcttttcaat
     2161 ggtccagggg tcggagacgc cgagccggtt ggcggaggct gcgatgagat tttcccgcac
     2221 cgtcagattg ggaaagatct ggcgtccttc gggtacgagg ccgatgccga gtttcgcgat
     2281 gcggtaggat ggcaggctgc tgacctcggt gccggcgaaa cggatattgc cggcgcgcgc
     2341 cggcgtcagc cccatgatcg aacggatggt cgtggtcttg cccatgccgt tgcggcccat
     2401 cagcgacacc atctcgcccg cgcgaatcgc cagcgaaagc ccgaacagca cctggctcag
     2461 gccgtagcag gtctcgatcg cgttcacctc cagcagcggc cgcgcgtcag ccatggcgaa
     2521 ccaccacgtg ctgatcgccg agataggcgc gtttgacctc ttcgttccgg cggatgtcgg
     2581 acggtgcacc cgcggcaatg acgcggccat agaccaggac cgagatgcgg tcggcgagcg
     2641 cgaacaccgc ctccatgtcg tgctcgacca gaatgatcgt gacctccttg cgcaagtcct
     2701 gcagcaattt caccatgcgg gcggattctg tcgggccgag cccggccatc ggctcatcca
     2761 ggagcaggag ctgggggctg gtggcgagcg cgaccgcaag ttcgagttgc cgctgctcgc
     2821 catggctcaa tctggaaacc agcatctcgg cacgatcggc aaggcccacg cgttgcagcg
     2881 ctgccagcgc agtccggcgc aggccttcct ccttgcgcgc gttgcgcaag aagcggaagg
     2941 agtgcccgtc acgcgcctgc gccgcgagcg cgacgttgtc ggcggcggtg aaatcggtca
     3001 gcagcgaggt gatctggaac gaacgcgcca ggcccagacc gctgcggcga taggcggcaa
     3061 gccgcgtgat gtcgcggccg cccaggaaga tgctgcccgc atgcggcatc aaatggccgg
     3121 tgagctggct gatcagcgtg gtcttgccgg cgccgttcgg cccgatgatc gcgtgcagct
     3181 cgccttgcgg tatctccagc gatacgtggt cggtcgcgat gatgccaccg aaacggcgca
     3241 cgagtttttg cacgcgaagc aggggctcag ccacgatcga acctcccgag caggcccatg
     3301 atgccgccgc gcccgaacag cacgatcatg agcaacagcg gccccatgat cagtgcccaa
     3361 tattcggtga cctgcgacaa taattcctcg agcacgagat aaaccacggc gccgatcgcg
     3421 ggaccgaaca gcgagcccat gccgcccagg atcaccatca ccatgagatc gccggaacgc
     3481 gtccagtaca tcacggcggg gctgacgaaa tcggtgttgt tcgcgagcag cgcaccggca
     3541 agtccgcaca tcgtgcccgc aatcacgaag cagaccagcc ggtaacgctt ggcggggaag
     3601 ccgatcgcct gcatgcgttg ttcgttggag cgcaggccct gcaccaccag gccgaagcgt
     3661 gaattgacga tgcgccagat gagatagatc acgccgagca agcaggcgag gcagagataa
     3721 tagaactgca cgcggttcga caggttgatc aagccggcga aggtgctgcg cttgtagatc
     3781 gtgagcccat cgtcccc
//
LOCUS       DATLJT010000335         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_937806,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000335 DATLJT010000000
VERSION     DATLJT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937806"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VM910_07665"
     CDS             complement(<1..1458)
                     /locus_tag="VM910_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HVI62455.1"
                     /translation="MLSISDISIRLAGRLLIDRSTVQIIPGARVGLVGRNGTGKSTLF
                     RAIRGELATESGSIAVPSRWRVGSLAQEAPDGPESLIEVVLEADLERHTLLREAETAS
                     DAHRIAEIQTRLVDIDAHSAPARAAAILSGLGFSTADQARPVGEFSGGWRMRVALAAT
                     LFAVPDLLLLDEPTNYLDLEGTLWLEDHLAHYPRTVIVISHDRDLLETSVDQILHLDR
                     GKLTLYRGGYSSFEEQRAQREMLDAKQAKRQEAERKRLQAFVDRFKAKASKARQAQSR
                     VKMLERMKPIAALVTQDVQEITFQHPEKMLSPPIIAVDEVSVGYDPQRPVLNRVTLRI
                     DNDDRIALLGANGNGKSTLVKLLADRLKPFSGSVTRAEKLGIAYFAQHQLDELNEDGS
                     PYDHIRALMPDAPESKVRGRAGAIGFSGKAADTSVKSLSGGEKARLLLGLATFFGPNM
                     IILDEPTNHLDIDSRAALAEAINDFPGAVIMVSHDR"
     gene            complement(1555..1737)
                     /locus_tag="VM910_07670"
     CDS             complement(1555..1737)
                     /locus_tag="VM910_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62456.1"
                     /translation="MPKITDPIEPIDRKTVRSICDAVGERLQQSLRPDFSGLTPYLRD
                     LLEELRKRDNDSRAGR"
     gene            complement(1883..2122)
                     /locus_tag="VM910_07675"
     CDS             complement(1883..2122)
                     /locus_tag="VM910_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur globule protein precursor"
                     /protein_id="HVI62457.1"
                     /translation="MLRKLSLVAVAAASLGAAALAPTSASAWGFHPGWHGGWYHGGPR
                     FYVGGPGYYGYGGCYVRELVPTPWGPRWRLVNRCY"
BASE COUNT          398 a          646 c          685 g          401 t
ORIGIN      
        1 gcgatcatgt gagaccatga tcaccgcgcc cggaaaatcg ttgatcgctt ccgcaagcgc
       61 cgcacggctg tcgatgtcga gatggttggt cggttcgtcc agaatgatca tgttggggcc
      121 gaagaaggtc gcaagcccca aaagcaggcg cgccttctcg ccgccggaca ggcttttcac
      181 cgacgtgtcc gccgccttgc cggagaagcc gatcgctccg gctcgccccc gcaccttcga
      241 ttccggcgcg tccggcatca atgcgcgaat atggtcgtag ggcgaaccgt cctcattcaa
      301 ttcgtcgagc tgatgctggg cgaaataggc gatgccgagt ttttccgccc gcgtgacgga
      361 gcccgagaac ggtttcagcc gatcggccag cagcttcacc agcgtcgact tgccgttgcc
      421 gttggcgccc aaaagcgcga tgcggtcgtc gttgtcgatg cgcaaggtca cgcggttgag
      481 cacgggcctt tggggatcgt agccgaccga gacctcgtcg acggcgatga tcggtggcga
      541 gagcatcttt tcgggatgct gaaaggtgat ctcctgcacg tcctgcgtca ccagggccgc
      601 aatcggtttc attctctcca gcatcttgac gcgcgactgt gcctgccgcg ccttcgatgc
      661 cttcgccttg aagcgatcga cgaaggcctg gaggcgcttg cgctccgcct cctgacgttt
      721 ggcctgcttg gcgtcgagca tctcgcgctg ggcgcgctgc tcctcaaagg aggagtagcc
      781 gccgcggtag agcgtgagct tgccgcgatc cagatgaagg atctgatcga ctgaagtttc
      841 cagcaggtcg cggtcgtggc tgatgacgat cacggtacgc ggatagtggg caagatgatc
      901 ttcgagccag agcgtgcctt cgagatcgag atagttggtc ggctcgtcca gcagcaaaag
      961 gtcgggtact gcaaacagcg ttgccgcaag cgcaacccgc atccgccagc cgccggagaa
     1021 ctcgccgacc ggccgcgcct ggtctgcggt cgaaaagcca agaccgctga gaatcgcagc
     1081 cgcccgggcc ggagcagaat gtgcatcaat gtcgaccagc ctggtctgga tctcggcaat
     1141 ccggtgcgca tcgctggccg tttcggcttc gcgaagcagg gtgtgacgct caaggtccgc
     1201 ctcgagcacg acctcgatca ggctttccgg cccgtcgggc gcctcctgcg cgaggcttcc
     1261 gacacgccaa cgcgagggaa ctgcaatgct cccgctttcg gtcgccagtt caccgcgaat
     1321 ggcgcggaac agggttgact tgccggtgcc gttacgaccg accaggccga cgcgcgcgcc
     1381 agggatgatc tggactgtgc tgcgatcaat caggagcctt cccgcgaggc ggatcgagat
     1441 atcggaaatc gagagcatgg gcgcttgtcg acgctcgcag cgtcaaacgc aaccgctttg
     1501 tgagcaaaaa gataccgccg agggcgcaac cagggaaaat tgctttcgca aggcctaacg
     1561 cccggcacgg ctgtcattgt cccgcttgcg cagttcttcg agcagatccc gcaggtacgg
     1621 ggtgaggccg gaaaagtcgg gccgcagact ttgttgcagc cgttcgccga cagcatcaca
     1681 gatcgatcgt accgttttgc gatcgatcgg ttcgatgggg tcggtgattt ttggcatggc
     1741 tgtcgtctgc aacgggctca tgccattaga agtcgcgggc gcgaaatttg tttccggtcc
     1801 gccgggtagc tttttcgtta ggatcgggaa ccagcccaat aggtcccggc ctggagccgg
     1861 gacttattgg ttgtcgcaaa catcagtagc agcggttcac cagacgccag cggggtcccc
     1921 agggggtcgg gaccagttcg cggacgtaac aaccgccgta accatagtaa ccgggtccgc
     1981 cgacatagaa gcgcgggcct ccgtgatacc agccgccgtg ccagcccgga tggaagcccc
     2041 aggcggaggc ggaggtcgga gctaaggccg ccgcacccag cgatgccgcg gcaactgcaa
     2101 caagcgagag tttgcgtaac atggtcgttt
//
LOCUS       DATLJT010000336         1842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3694720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000336 DATLJT010000000
VERSION     DATLJT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VM910_07680"
     CDS             complement(<1..638)
                     /locus_tag="VM910_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI62458.1"
                     /translation="MPSEIESRTHDPVAATASAPAQALLSMQGITKSYGDHVVLNHID
                     LDVGPREKVAVVGPSGSGKTPILRAAIGLIKPDEGTIWIDGEYLWHVKATDGLRPAGE
                     RHARRVRRAVGIVFQQFNLFPHMTALENVREPLVHALGMRRQESDARATDLLCQVGLK
                     DHLGKHPAQLSGGQQQRVAIARSLALRPKIMLFDEVTSALDPELVGEVLRVVR"
     gene            complement(622..1287)
                     /gene="ehuD"
                     /locus_tag="VM910_07685"
     CDS             complement(622..1287)
                     /gene="ehuD"
                     /locus_tag="VM910_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HVI62459.1"
                     /translation="MSSFHWDWGFAWAILPELLQGLVVTVVATLLGSMVAFALGMVWA
                     FVRVLRISLLTPSVSFFVEFIRGTPLLIQLYFLYYVLPTWGLTISALPTGIFGLGLFY
                     SAYAAEIFRAGIEDLPAGQWEAALTLGLPVRRVWLGIILPQAIRSVVPILGNVIISMF
                     KQSSLLSTITVFELLAAGLNIGSVNYRYVEPLTLVGAFYFAISFLSARIVRSLEAKHA
                     FGD"
     gene            complement(1284..1643)
                     /locus_tag="VM910_07690"
                     /pseudo
     CDS             complement(1284..1643)
                     /locus_tag="VM910_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(1659..>1842)
                     /locus_tag="VM910_07695"
     CDS             complement(1659..>1842)
                     /locus_tag="VM910_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI62460.1"
                     /translation="VIEFFRGSSALVQLFWAFYVLPFFGIQLPPLVAGILVLGLNEGS
                     YFSEVVRAGLDAVPKG"
BASE COUNT          443 a          562 c          555 g          282 t
ORIGIN      
        1 cgcacgacgc gcaggacctc gccgaccagc tccgggtcga gagcggaggt gacttcgtcg
       61 aacaacatga tctttggcct gagggcaagc gaccgcgcaa tggcgacgcg ttgctgttga
      121 cccccggaca gctgtgccgg atgcttgccg agatgatcct tgagaccaac ttggcacaaa
      181 agatcggtgg ctcttgcatc gctctcctgg cgccgcatcc ctagcgcgtg aacgagcggc
      241 tcgcggacat tctccaacgc cgtcatgtgc ggaaaaagat tgaattgctg aaacactatg
      301 ccaaccgcgc ggcggaccct gcgggcatgc cgctcgccgg ccggtcgcag accgtcggtc
      361 gccttgacat gccaaagata ctcaccatcg atccagatgg tgccttcgtc cggctttatg
      421 agacctatgg ccgcgcgcag aatcggggtt ttgccggatc cgctcggccc gacaaccgca
      481 actttctcgc gcggccccac gtcgagatcg atgtggttga ggaccacgtg atcgccatag
      541 ctctttgtga tgccttgcat tgaaagcagc gcctgcgcgg gcgcgctcgc cgtcgccgcc
      601 acaggatcgt gggtgcgcga ctcaatctcc gaaggcatgc ttcgcctcca aggagcggac
      661 gatccgagct gacagaaagc tgattgcgaa ataaaacgcg ccgacaagag tcaacggctc
      721 gacatatcgg taattgacgg aaccgatatt cagtcctgcg gcgagcagct cgaacacagt
      781 gatcgtcgaa agaagcgagc tctgcttgaa catgctgatg atgacattac ccagaatcgg
      841 cacgaccgat ctgatggcct gcggcaggat gatgccaagc cagacgcgac ggaccggcaa
      901 gccgagagtg agcgccgcct cccattgacc ggcaggaagg tcctcgatgc cggcgcgaaa
      961 gatttcggct gcgtaggcgc tataaaacaa tccgagcccg aaaatgcccg tgggcaaggc
     1021 ggaaatcgtc aggccccatg tcggcagcac gtaatacagg aagtaaagct ggatcagcag
     1081 cggggtgccg cgaatgaatt cgacgaagaa tgaaacggac ggtgtcagga gggagatacg
     1141 cagcacgcga acaaaggccc agaccattcc caacgcaaaa gccaccatcg agccgagcaa
     1201 ggtcgccacc acggtgacga cgagcccctg cagaagctct ggaaggattg cccaggcgaa
     1261 gccccagtcc caatggaatg aactcacctc gtgcccccca ccgcccaatc gggaatcggg
     1321 gagcgccgcg ccgcgggagc cacggaaaga tcacggcctg cccagcggct gaccgggcgc
     1381 tcaagcagcc ggacgccgcc gcccagcaga agcgccagaa cgaaataaat gatgatggtc
     1441 aggccgaata tcggaccgct ttcgccaagc gtgctgctga tgaggctcgc gcgaaacgac
     1501 aggtcctgga ccgtcaccag cgaaaccagc gcggtaaatt tcaacatcgt gatcagttcg
     1561 ttcccgaacg gcggcaccat gatcggaagc gcctgcggca ggattattcg aaacaggcga
     1621 tagtgaaacg ggaggcccag tgtaaccgcg gcttcgcgct agcctttcgg aaccgcatca
     1681 agaccggctc ggaccacctc agaaaagtag ctcccctcgt tcaatccgag gacgagaatg
     1741 ccggcgacca acggaggaag ctggatgccg aaaaacggca gcacgtagaa tgcccagaac
     1801 aactggacga gcgccgagct cccgcggaag aactcgatga cg
//
LOCUS       DATLJT010000337         1600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_18792,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000337 DATLJT010000000
VERSION     DATLJT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18792"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VM910_07700"
     CDS             <1..199
                     /locus_tag="VM910_07700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HVI62461.1"
                     /translation="GIACIGMGGAGVGVGIIFGNYLAAAIRNPSAAAGQFANLIFGFA
                     ITEALGIFSLLIALLLLFALG"
     gene            259..810
                     /locus_tag="VM910_07705"
     CDS             259..810
                     /locus_tag="VM910_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HVI62462.1"
                     /translation="MAQDKPQPVGAVEVPASEHGRGFPPFDSQTFPSQLFWLAIAFIA
                     LYLLMSRIALPRIGSILEARRKRVEDDLAEAQRFKDGSDAAIAAHEKALAEARGRAQA
                     LANETRANAAAAAEARRKEVDAKLHAHIAEAEKTIAEKRAAAMSNVRNIAGEAAGAIV
                     ERLTGISPASEEVAQAISQALKR"
     gene            819..1301
                     /locus_tag="VM910_07710"
     CDS             819..1301
                     /locus_tag="VM910_07710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195257.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HVI62463.1"
                     /translation="MFEAEFWVAVAFVIFIGVLGYFRVHKLLLKSIDDRRERIKAEFD
                     EARRLKAEAEALLATYRRKQQEAEHEAQAIVAGARTEAERLAAEAEAKIEEFVVRRTK
                     MAENKIAQAEAQALADVRSAAAEVAVAAAKTILTRTVKDKVADNLLTKGIEDFKGKLN
                     "
BASE COUNT          302 a          506 c          515 g          277 t
ORIGIN      
        1 cggtattgct tgcatcggca tgggtggcgc cggcgtcggc gttggcatca tcttcggcaa
       61 ctatttggcc gcggcgatcc gcaacccgtc ggccgcggcc ggacagttcg ccaacttgat
      121 tttcggcttc gccatcaccg aggcgctcgg catcttctcg ctgctgattg cgctgcttct
      181 gctgttcgcc ctcggttgat cgtggcatcg aacgcgtggg cggcagccga acgcgccccg
      241 tagcctgtgg aggcggccat ggcccaggat aagccccagc ctgttggagc cgtcgaggtc
      301 cccgcgagcg agcacggtcg cgggttcccg ccgttcgact cgcagacctt cccttcccag
      361 ttgttttggc tcgcgatcgc cttcatcgcg ctctatctgc tgatgtcgcg aattgcgttg
      421 ccgcgcatcg gctcgatcct ggaggcccgc cgcaagcgcg tcgaagacga ccttgcggaa
      481 gcgcagcgct tcaaggacgg gtccgacgcc gccattgcgg cccacgagaa ggcgttagcg
      541 gaggcccgcg gacgggcgca agcgctcgcg aacgagacgc gcgcaaatgc agccgcagca
      601 gccgaagccc gtcgcaagga ggtggacgcg aaactccacg cccatatcgc cgaagcggaa
      661 aagacgattg ccgagaagcg ggcggcggca atgagcaatg tccgcaacat cgccggcgag
      721 gctgcgggcg cgattgtcga gcgtttgacc ggtatatcgc cggcgagcga agaggtcgcc
      781 caagccatca gccaggccct caaacgctga ggaagccgat gttcgaagcc gagttctggg
      841 tcgcggtcgc ctttgtcatc ttcatcggcg tgctcggtta ttttagggtg cacaagctct
      901 tgctcaagag catcgacgac cggcgggaac gcatcaaggc ggagttcgac gaggcgcgca
      961 ggctcaaggc ggaagcggag gccctgcttg ccacatatcg acgcaagcag caggaagccg
     1021 agcacgaggc gcaggcgatc gtcgccgggg ccagaaccga ggccgagcgg cttgccgccg
     1081 aggctgaggc caagatcgaa gaattcgtcg tgcggcgtac caagatggcg gaaaacaaaa
     1141 tcgctcaggc cgaagcccag gcgctggccg acgtgcgctc tgcggccgcc gaggtcgcgg
     1201 tcgccgcggc caaaacgatc ctcacccgca ccgtcaagga caaggttgcc gacaatctcc
     1261 ttaccaaagg catcgaagac ttcaagggca aactaaacta gtcttttttc cggccacttt
     1321 cggccacgac gcacgattat tccggctttc cccgcaaaac gcagaagctg tgtaaaaaaa
     1381 tctctcgggc gcgcggcgcc cgacttttgc catgggccca cgattccttc agttgcgccg
     1441 cgagtcgttg aaaccaccgc gcggtgctgc aagcacgccg gcgtggggct cccgtggtta
     1501 tctttgattg caatggtgtg ctggtcgaca gcgaacaaat cgccgcagcg gctgccgcgg
     1561 acgaatttgc gcgggctgga attccgatta cgcccgagct
//
LOCUS       DATLJT010000338         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1626986,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000338 DATLJT010000000
VERSION     DATLJT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626986"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1347
                     /locus_tag="VM910_07715"
     CDS             127..1347
                     /locus_tag="VM910_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI62464.1"
                     /translation="MDSLIAAAARALATGDLLGALKRVALRDDAPALALRGIAMARLG
                     DLPRAKTLLKSAARAFGPREAVARARCVVAEAEIALVSRDLAFPAKALDTARATLEAH
                     GDRLNAAHAQHLAIRRLLLIGRLDEAERKLAGLDPAPLPAASMAAHELVVAGIAIRRL
                     QIKTARLALTTAARAAREAKIPALLAEVASASNVLNTPAARLIARGKERPLLLEEVEA
                     TLASKALVVDACRHVVRDAGKTVSLATRPVLFALARALGEAWPSDVSRAILIARAFRG
                     KEADESHRARLRVEMGRLRAALRTLADVSATRDGFALAPRTAREVVVLARPVEEEHAA
                     VLAFLADGESWSSSALALALGTSQRSVQRALDELAASGKVQSLGQARARRWMTPPTSG
                     FTTTLLLPAPLPAD"
     gene            1386..2024
                     /locus_tag="VM910_07720"
     CDS             1386..2024
                     /locus_tag="VM910_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVI62465.1"
                     /translation="MKRSAAEIVAEYGPFPGVERVNGVTYDGQHVWFASGDKLNALDP
                     ASGKTVREIDVAAHAGTAFDGQHLFQIAEDRIQKIDPRTGRVLSTIPAPGRGRDSGLA
                     WAEGTLWVGEDRARKIHQIDPQTGAILRTIESNRFVTGVTWVDGELWHGTWEGEESDV
                     RRVDPKTGEVLERLEMPAGTGVSGLESDGRDRFFCGGGSSGKVRTVRRPKRS"
BASE COUNT          422 a          762 c          842 g          394 t
ORIGIN      
        1 gtaaggtacg ttggggaaag gatcgtccag tcaaccatgg cggtcgcctt ctcgctggtt
       61 tgtgagcgtg agatagaata ggaccggaca tcgaaaggtg agagtgacaa gtgtggcggg
      121 attccgatgg actcgctgat cgcggccgcg gcgcgtgcgc tcgccacagg tgatctcctc
      181 ggcgcactga aacgggttgc cttgcgcgac gatgcgcccg cgctcgcgct tcgtggcatc
      241 gcgatggcgc gcctcgggga cctgccccgt gcgaaaactc tcctgaaaag cgcggcgcgc
      301 gccttcgggc cgcgagaggc cgtggctcgc gcgagatgcg tcgtcgccga ggccgagatc
      361 gcgctggtct cgcgcgacct ggccttcccc gcaaaagcgc tcgatacggc gcgggccaca
      421 ctggaagcgc atggcgaccg actgaacgcg gcacatgcgc agcatcttgc gatccgccgc
      481 ctgctcctga tcggtcgcct cgatgaggcc gagcgcaagc tcgccgggct cgaccccgcg
      541 cccctcccgg ccgcctcaat ggcggcacac gagctggtgg ttgcagggat cgcgatcagg
      601 cgtctgcaga taaagacggc acggcttgcg ctcaccaccg ccgcgcgcgc cgcacgcgaa
      661 gcaaagatcc ctgcgctgct ggcagaggtc gcaagcgctt caaacgttct gaatacgccg
      721 gcagcgcgct tgatcgcacg tgggaaggag cgcccgcttc tgctcgagga agtcgaggcg
      781 acgctggcgt caaaggcgct cgtcgtggac gcgtgccgtc atgtcgtgcg cgacgccggc
      841 aaaacggtct cgctcgcgac gcgtccggtg ctgttcgcac tcgcccgcgc gctcggcgag
      901 gcctggccat ctgacgtgtc gcgggcgatc ctgatcgccc gggcctttcg gggcaaggag
      961 gcggacgagt cgcatcgtgc gcgattgcgg gttgaaatgg ggcggcttcg cgcagcactt
     1021 cgcacgctgg ccgacgtgag tgcgacaagg gatggttttg cattggcgcc gcgcaccgcg
     1081 cgcgaggtgg tcgtgctggc gcggcccgtc gaggaggagc atgcagcggt gctcgcgttc
     1141 ctcgccgacg gcgagtcctg gtcgagttcg gcgttggcgc ttgcgctcgg cacgagccaa
     1201 cgcagcgtgc agcgagccct cgatgagctg gcggcctccg gcaaggtgca gtcgctcggc
     1261 caggcacggg cgcgtcgctg gatgacccct cccaccagtg gattcacgac gaccttgtta
     1321 ctccccgctc ccctgccggc tgattaaggt ggacccacga taaagagagt tgcgaggaga
     1381 gacagatgaa acgatcggcc gccgaaattg tcgctgagta cggtcccttt ccgggcgtcg
     1441 agcgcgtgaa tggcgtcacc tatgacggac aacacgtctg gtttgcgtcg ggcgacaagt
     1501 tgaacgcgct cgaccccgcg agcggaaaga cggtgcgtga gatcgatgtc gcagcccatg
     1561 ccggaacggc gtttgacggc caacatctgt ttcagattgc cgaagatcgc atccagaaga
     1621 tcgaccccag gaccggccgc gtgctctcca cgatcccggc ccccggccgc ggccgtgact
     1681 ccggcctcgc ctgggccgaa ggcacgctct gggtcggcga ggatcgggcg agaaaaattc
     1741 atcagatcga cccgcagacc ggggcgatcc tccgcacgat cgagtccaac cgcttcgtca
     1801 ccggcgtcac ctgggtggat ggcgaactgt ggcacggcac ctgggaaggc gaagagagcg
     1861 atgtgaggcg ggttgatccc aaaaccggcg aggtgctgga gcggctcgaa atgccggctg
     1921 gcacaggcgt ctcgggactc gagtccgatg gacgcgatcg cttcttctgc ggcggcggca
     1981 gcagcggtaa agtgcgcacg gtgcggcgac cgaagcggag ctgaattgcg gggcagaaaa
     2041 ggcggcagcg cgcgtaccgc cctgaaaggg ttggatacat agtggacgcg gcgctgactc
     2101 gccaaggtcc tggcgaagcc gtggcccttc atcgatcacg tgaagggtcg aacaccggca
     2161 actgatttgc ctgacaggca aatcagttgt tgccggactc atgccgggcg gaagtgcaag
     2221 cgctcttgca cacatgtcgg ccatgcgcaa attgcccgtc gtgccaatct gccgcagctc
     2281 gccgacgttg ctccggaccc caaatcaaca tcattttccg cgcgtcccgc gcccgataga
     2341 ggggcgcttc gcgatcgtca cgagtgttgg gcacgggatg cgatggacgc ggcagcgcgt
     2401 gcgacgagcg cggctgaagc
//
LOCUS       DATLJT010000339        25229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3924419,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000339 DATLJT010000000
VERSION     DATLJT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 25229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3924419"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VM910_07725"
     CDS             <1..489
                     /locus_tag="VM910_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI62466.1"
                     /translation="ALVRCNKPMVAAVSGVAVGVGTTMLFHCDFVVAGSDAYFSTPFV
                     SLGLIPEAASSLLAPRLMGQRRAFEFLVMGHPLNATHAKEFGIANIVVAPEDVDAEGL
                     KAAREIAALPAGAVALSRALMRGATDELLARIDQEAKIFSERLNSPEAKAAFEAFFAR
                     KR"
     gene            495..614
                     /locus_tag="VM910_07730"
                     /pseudo
     CDS             495..614
                     /locus_tag="VM910_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(646..848)
                     /gene="gudD"
                     /locus_tag="VM910_07735"
                     /pseudo
     CDS             complement(646..848)
                     /gene="gudD"
                     /locus_tag="VM910_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046338.1"
                     /note="catalyzes the formation of
                     5-keto-4-deoxy-D-glucarate from glucarate; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
     gene            1136..1406
                     /locus_tag="VM910_07740"
                     /pseudo
     CDS             1136..1406
                     /locus_tag="VM910_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
     gene            1519..2472
                     /locus_tag="VM910_07745"
     CDS             1519..2472
                     /locus_tag="VM910_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HVI62467.1"
                     /translation="MAKFRSILQTVGNTPVVRINNLAPRGVNLYVKIEAFNPLGSVKD
                     RLALGVIEAAEQSGQLKPGQTVIEATSGNTGIGLAMVCAQKGYPLVVTMAEQFSVERR
                     KLMRFLGAKVVLTPAVGRAWAMVTKAVELASTHGWFLTRQFENEANADFHSRTTAREI
                     IADFKGEKLDYWVTGYGTGGTLKGVSRVLAKEMPDTKIIVCEPEDAQLLASGVEQARN
                     ADGSPASPHPAFKPHPMQGWTPDFIPKLTADAVKMGVIDRVLAIKGSDAILYVGSWRA
                     GKASSWVSPLEQPSPAPCTCAVRRQRMGTSFACCPIPVSAI"
     gene            complement(2849..3121)
                     /locus_tag="VM910_07750"
     CDS             complement(2849..3121)
                     /locus_tag="VM910_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62468.1"
                     /translation="MRFNDNVFRGSRQNYDLHQIPCAARPSGRGRAPPAPKNGWQKVP
                     HNDGHLSLKRALASRLSGEWSDDDYDVLADRVVVGRIMKVAAHVLR"
     gene            3157..4386
                     /locus_tag="VM910_07755"
     CDS             3157..4386
                     /locus_tag="VM910_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HVI62469.1"
                     /translation="MATNLRINGERLCWRLDRLAQIGRIAGDGVCRLALSNEDKQGRD
                     LVVGWMRELGLSVRVDKIGNVIGTRRGLEDGPPVMTGSHIDSVRTGGRYDGVLGVLAG
                     LEVIATLNDAEFHSRRPIAVAFFTNEEGSRFQPDMFGSLVYTGGLKLDQAVATRGIDG
                     VSVGEELDRIGYSGPEPVGRPQAHAYVELHVEQGPVLEAENFRIGAVVGVQGISWTEF
                     TLSGQSNHAGTTPMRMRRDAGYVAAQIAVFARSLAKEIGGNQVATVGLMTLSPNLVNV
                     IANEVVMTVDLRNTDDEQLREAERRLFAFADKAAAAEKVGIARRSLARFAPVAFAPHL
                     VERVEAIAAAFGFPVRRLPSGAGHDAQIMAAVCPSCMIFVPSVGGISHNVEEYTAPAD
                     LVAGADVLLRLVLELAE"
     gene            4917..6032
                     /locus_tag="VM910_07760"
     CDS             4917..6032
                     /locus_tag="VM910_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62470.1"
                     /translation="MQEVVRREAELTLGLSTGLVYNPAVYCDQREITALVRAFNGVKP
                     GALFPHLRSESDNIVASLKEVLEAAIDGGGGYCNEHSKIAGERNYGKFGQLESMLDDA
                     RSLIPTMENMYPYAAGSTTGDAIFPPEARAGSRATFLARLREPTVRRAMADKMRHDTT
                     SWDNLVSFCGGLQGIQIAGVKPNVGARYLGKRLSDVAFEAGAPDSDSDAAYDAVFDFF
                     ADNDGDIAIITHYGNEATVERFFRRPNMAICTDGLMPGPGQKLHPRSLGAFPKALRMG
                     RDMGIPLTQMIWRLSTLPCRFLNLPDPTLKIGSDASLVLFDPNTVRERNDYLDPLVSP
                     DGIDFVWVHGKLVLELGKLLAPQRFPGRILTSQLKAA"
     gene            6433..7806
                     /locus_tag="VM910_07765"
     CDS             6433..7806
                     /locus_tag="VM910_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HVI62471.1"
                     /translation="MESNQKPTVAPVDEILPLPKLAALGFQHVLVMYAGAVAVPLIIG
                     RALKLSPEQVALLINADLFACGIVTLIQSFGAGAWFGIRLPVMMGVTFAAVGPMLAMA
                     NDPSLGILGIFGAVIAAGVFTIIVAPAISYFLPFFPPVVTGSIILMIGISLMRIGVNW
                     AAGASVPTAPNYGDPFNLGMAAFVLVCILLCTRYATGFVRNIAVLIGLIGGCIVAYLL
                     GKMNFGKVIDAPWFSLIYPLQFGMPKFEIVSIITMCIVMIVVMIESAGMFLALSDMTG
                     KRLEAIDMTRGLRTDGLGTLIGGLFNTFPYTSFSQNVGLVGVTGIKSRFVCVTGGIIL
                     LAMGLVPKLGALVEAVPLFVLGGAGIVMFGMVAATGIRILSGVDYQTTRNNLYVVAIS
                     LGFGMIPLVAPTFFAKLPAQLGPILNSGILLTAIAAVLLNFYFNGVSDRTTAVSAARS
                     AATLSEA"
     gene            8168..9358
                     /locus_tag="VM910_07770"
     CDS             8168..9358
                     /locus_tag="VM910_07770"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI62472.1"
                     /translation="MALNKAILIGPDRNGRCVAVENGLVAALPPKNARVLSCAGGEIR
                     AGAVCAHTHLYSGLSRYGMPAASPPPGNFLQLLERVWWRLDRALDETTLRAAAMDYVA
                     RALLAGTTALVDHHESPNLIEGSLAILAEVCEQLGMRALLCYGATERNAGRDEARRGL
                     AECRRAPASPLVRGLVGLHASFTVSNDTVRACGELARELGTVLHVHVAEDSADVEDAR
                     SRGFAGPLERLSKLGALVPGSILAHGVHLNREQAEMAGSLSCWLVQNPRSNEANRVGY
                     ASCLQYASKVALGSDGWNGDMAEEQDALFRLAELHGDRRADGRLAAGHALIAERFGSE
                     PEPLSQGSLGDVVVWENGRVRHSVVGGRVVVEDGELTGSAIDEISAVAQSSAVRLWDR
                     MRMM"
     gene            9455..10702
                     /locus_tag="VM910_07775"
     CDS             9455..10702
                     /locus_tag="VM910_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVI62473.1"
                     /translation="MIDAVHALPFRCPGHLPGDDIDHVLAPDAIDGVFPAGTEHNPFL
                     RYRTLLSPYRLACAAGLSDAGWTDIVETLDAAVLGIAGGGFRITPMTEQSRLAAALGF
                     RDGLWVKDETGDVAGSHKARHLMGVMLHLRVLELAKLPAGKDLRARRLAIASCGNAAL
                     AAAVIARAADWPLDVFIPPGADSAVKQKLIELGANITTCQRGHGGAGDPCFLHFREAV
                     AAGAIPFSVQGTENGLAVEGGRTLAFEMIEQFRGAGIGPDQLFVQVGGGALASALAQG
                     FALGLRFGMLAKLPRLIAVQTHACAPLARAWRKVDGLDLASVARHRSHFMWPWEGTTV
                     SLASGIVDDETYDWWAVVEGMRGSGGRPVLVDETMIARAHEICRLHTGISASPTGTAG
                     LAGAMALPAEKGRTAVILSGVER"
     gene            complement(10902..11918)
                     /locus_tag="VM910_07780"
     CDS             complement(10902..11918)
                     /locus_tag="VM910_07780"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HVI62474.1"
                     /translation="MTDLTTRYLGLTLRSPLVASSSPLADSVENIVRLEDHGIAAVVL
                     PSLFEEQLELESRSVDHDLARGSESFAEALSFFPDMQTYNLGPDRYLELIRRAKERVD
                     IPVIASLNGVTPGGWTQYARLMEEAGADAVELNIYAIATDPLQPGEDVERDQRELVTE
                     IRRSLRIPMAVKLSPFFSAPANMGLRLDQAGANALVLFNRFYQPDFNIDDLEVVPTLS
                     LSRSEELLVRLHWTAILYGHVRADLGITGGVHSAEDVVKCVMAGARVAFMASALLKNG
                     IRHASQVLSDLARWLDAHEYVSIAEMCGSMSYDAVPNATAYERGNYMKVLSSYTLKTR
                     ALHE"
     gene            complement(11915..15541)
                     /gene="nifJ"
                     /locus_tag="VM910_07785"
     CDS             complement(11915..15541)
                     /gene="nifJ"
                     /locus_tag="VM910_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HVI62475.1"
                     /translation="MAQARAVVDGNEAVASVAYRLNEVIAIYPITPSSTMGELADQWK
                     AGGATNIWGTVPDVIEMQSEGGAAGALHGALQGGALATTFTASQGLLLMIPNMYKIAG
                     ELTAAVIHVAARTIATHALSIFGDHSDVMAARTTGFALLAANSVQEACDFALVAQAAT
                     LESRVPFLHFFDGFRTSHEIATIESLSDDDLRAIIDNAFVVQHRERAMSPDRPVLRGT
                     AHNPDMFFQAREASNPYYRDCPTIVQDVMDRLARRTGRAYRLFDYYGAPDADRVLVLM
                     GSGAGAAEEVVDALQERGERVGIVKVRLFRPFAIENFLAALPATVRSIAVLDRTKEPG
                     SIGEPLYQDVITALMERHGSGAGATMQRVIGGRYGLASKEFTPAMVKAVFDELAKAHP
                     KRHFTVGILDDITHTSLDCDTEFSTEHPRTVRAVFFGLGSDGTIGANKNSIKIIGKQT
                     RNYVQGYFVYDSKKAGAVTVSHLRFGLHPIKSTYLIGKANFVACHQFQFLKRIDVLLA
                     AEPGATFLLNAPFGADEIWDHLPGHVQDTILRKKLKFYVIDAFAIARECGIGTRINTI
                     MQTCFFALSGVLPRDEAIAAIKEAIEDTYGKRGSAAVQKNFAAVDRTLTRLHEVPLPL
                     PHQPSGSFDMPIPVAPAAPEFVKNVLGKAIAGRGDTLPVSAMPVDGTFPTGTAKWEKR
                     NIAQQIPVWDLDLCIQCGKCVLVCPHSVIRAKIYDASHIARAPSSFKAAKPKWREFES
                     MRYTLQVSPQDCTGCNLCVEACPVRSKSEAKHKAINMEPQAPLCDTESTNWEFFLSLP
                     ELDRGRLSHDTVKNVQVLEPLFEFSGACAGCGETPYIKLLTQLFGDRLLIANATGCSS
                     IYGGNLPTTPYTANAQGRGPAWSNSLFEDNAEFGLGMRLAVDKQTEFAREMVARLSAD
                     LGDALATGILQADQSTEAGIDEQRRRVAELKRKLVTMGSSDAAALRVVADALVRRSVW
                     LVGGDGWAYDIGAGGLDHVLESGRNVNVLVLDTEVYSNTGGQMSKATPRGAIAKFSTG
                     GKHTAKKDLAMEAVNYGSVYVARVAMGGNDIHAVRTFLEAESYDGPSIIIAYSHCIAH
                     GYDMSFGLAQQRSAVLSGYWPLMRYDPRLQQDGKNPFQLDSMPPSIAFRDYAYQEARY
                     TMLARSNPEAASALLVRAQQDVERQWRIYANRAAMAGHVAPADNAPSESQVSALPATE
                     SRSL"
     gene            15817..16026
                     /locus_tag="VM910_07790"
     CDS             15817..16026
                     /locus_tag="VM910_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVI62476.1"
                     /translation="MAYQIVASLCTSCGACELECPNSAISIGRFTYVIDPNKCTECKG
                     FYDQPHCATVCPVPNTCVPASPQGN"
     gene            complement(16292..18931)
                     /gene="xdh"
                     /locus_tag="VM910_07795"
     CDS             complement(16292..18931)
                     /gene="xdh"
                     /locus_tag="VM910_07795"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HVI62477.1"
                     /translation="MKVVKFHLNGTPREVEAWPDEPLVDVLRDIFKIKSVKAACGPQK
                     ECGCCLALIDGLPKVTCSVRIAQVEGRSILTLEGVSDNERRLYADAFQAAAGLQCGFC
                     TPGFVLRIKYLTDQQSALSRAEMARALDGHLCRCTGYVKIVDAFELIQTAKRGGALPA
                     PVDSGGVGQPLRRYQGGELALGTRPFVADIDVAGLLHGVVVLSPHARARVIRIDTTKA
                     LALQGVIKVATAKDVPGDRWVGQIYKDWPCFVGEGEEVRYVGDVVAAVAAETPRIARQ
                     AANLVEVEYEALPPVLDPAEAIKLGAPQVNPKHGNVLSVTRYARGDVEAALDASAHVV
                     SGTWATQRIEHLFLEPEAAVAVPLPGGGLHIYSQGQGIFEDRRQVASVLGEPEDRLFV
                     ELMPNGGGFGGKEDMTIQAQTALLARMTGRPVRIVLNREESIRMHPKRHPLTMTYTVG
                     CDAYGLLTAAKVNILGDSGAYASVGSKVLERAAGHACGPYRVPAVAIEAVAAYTNNPP
                     CGAMRGFGVNQTSFAIEGCLDLLAAKAGIDRWEMRWRNVLRIGDAFTTGQILEKSVGI
                     EKTLLAVKDAYDVARAQGRAVGIACGVKNCGLGNGAVEYGKCRLVVQPGGTVELYTGF
                     TEMGQGLLTILTQCAVEATGLPAGIFRPRVSSRFELGAGQTTGSRASLLGGRAVVDAA
                     IKLKADLNRGLTLADLAGRIYAGETRIDDTTAPGEMKNGKIKTHTTFGWATQVVVLSG
                     NGAVERVVAAHDVGRALNPQQCEAQIEGAVHMGLGYALTEELPCKHGMPVTFKLRDIG
                     VLRAQHMPEVKVILVEDTEPEGPFGAKGVGEIGLVPTAGAVAGALEAFDAVRRTRLPM
                     KDSPSARAINLGHIPDGERDQWH"
     gene            complement(18959..21019)
                     /locus_tag="VM910_07800"
     CDS             complement(18959..21019)
                     /locus_tag="VM910_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62478.1"
                     /translation="MVQLVPIPFGVLVSRLFCELERKRSAFDLPSQRFVQGNPRHDLS
                     VAIHGRRASTPFGPAAGPHTQMAQNIVLSWLAGGRVIELKTIQVKDDLTIPRPCIDVA
                     TVGFNVEWSQELTVDKSLEEYVKGSMLIEMLKASGLAPGLGETIFDMSVGYDLAGIRS
                     AKVQNFLATMMDATPVVERLRAQIPEAFSHLRALPFTSRISDTLTLSTFHGCPPEEIE
                     SIARFLLTEVGINVMVKLNPTLLGRDELLSILHERIGYTELKVPDAAFAKDANWQQVT
                     ELVERLSPEARALGRGFGVKFSNTLVVANHKSFFPASQQEMYLSGPPLHPLAIALVRR
                     FRKRFGDRFPISFSAGIDEINFADTVALGLKPITVCTNLLKHGGYRRGWHYLGDLIKR
                     MDEVGAADIDTFVLKAYGQAEAALARLALSDEKSAACRAALVVGGDLRTTAGEAFATW
                     VSVARLLNTEVYGERVLADRRYCAAENSTPPNKIGSKLVLFDCLTCDKCIPICPNDAN
                     FTFVIPTGEVPIQRLAWSEDGWTSETVGALRFTKQRQIGTFADVCNECGHCDVLCPED
                     GGPYLVKPLFFGSIDAWAASPHRDGFVLQRIVNGIAMHGRFGGRVVTMEIGNGLVRYR
                     GEGFDVRLDPSEPAATVSGTADGPVDLTWLHIMDQILRAVTAPKAVNYIGTILACSPV
                     GSPA"
     gene            complement(21228..22685)
                     /gene="hydA"
                     /locus_tag="VM910_07805"
     CDS             complement(21228..22685)
                     /gene="hydA"
                     /locus_tag="VM910_07805"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HVI62479.1"
                     /translation="MTSLIRGGTIVNHDHSQRADVLICDGLITAVGASLEAPPGTETI
                     DAGGCYVMPGGIDPHTHLELRSIGGAVSADNFEWGCKAALSGGTTMVVDFCTPNPGQT
                     LLAAYQDRRREAEGAACDYGFHMAITWWDKQVFDEMETCVKTYGINTFKHYMAYKGAL
                     MVNDDDLYNSFVRCASLGAIPLVHAENGDVVWRLQQHYLDDGVTGPEGHAYSRPPGVE
                     GEAANRAIMIADVAGCPLYVVHISCREAHDAITRARAAGKRVFGEALIQHLLLDEDEY
                     RNEDWDHAAQRVMSPPLRTKDHQASLWAGLQSGSLQVVATDHCTFTTAQKRLGRIDFT
                     KIPNGTGGLEDRMRVLWTAGVNSGRLTKEEFVAVTSANSARILNIFPKKGAVAVGSDA
                     DLVIWDPRATTTISAKKQVSRIDYNVFEGFFCTGQPHATLSRGEISWLDGELRARAGA
                     GKYVPRDPLPGVHVANTTWTEFTTARRVKRNDVAS"
     gene            23028..23351
                     /locus_tag="VM910_07810"
                     /pseudo
     CDS             23028..23351
                     /locus_tag="VM910_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            23469..23876
                     /locus_tag="VM910_07815"
     CDS             23469..23876
                     /locus_tag="VM910_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HVI62480.1"
                     /translation="MSTPEPYWGVLQLQANRTKLALHCLELAGYAVYHPRIRERRVVR
                     GRWTSVLCPLFPGYCFLHIHLQWHSARWAPGVVRLVLNGGAPARLSEPAIAAIRQRER
                     NGLVELPRRPLQRGDKVRVRRGPFEGHSASMTA"
     gene            complement(23949..24533)
                     /locus_tag="VM910_07820"
     CDS             complement(23949..24533)
                     /locus_tag="VM910_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62481.1"
                     /translation="MIALVVFWTAPAQAQNYPAKPIHIMVPYAPGGISDIASRILGAK
                     LTEAWGQQVLVENRPGGNGFIAMAATAKAAADGYTLVMATGGDVAINPAMFKEMPYDV
                     ERDLAPIAMVSDAPLVLAAHGGAPYNSVADVIAAAKAQPGRISVATPGNGSINQIVLE
                     WMALNTGTQFQHIPYKGFCITSAMVETNIQINVL"
     gene            complement(24645..>25229)
                     /locus_tag="VM910_07825"
     CDS             complement(24645..>25229)
                     /locus_tag="VM910_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI62482.1"
                     /translation="GFLLAAAGGVGLAVLFNQSRLIEYSLYPYAVILQVTPIVAIAPL
                     LLVYLPQQAAVLACAWIVAFFPVLANTTLGLRSVDHNLADLFRLYGATRRQLLWELKL
                     PAALPYILGGLKIAGGLSLIGAVVAEIAAGSAGAGSGLAYRIVESGYRLNIPRMFAAL
                     LLLSAAGIVIFFALSLVSHLLLRRWHESAVVREV"
BASE COUNT         4919 a         7628 c         7703 g         4979 t
ORIGIN      
        1 gcgctcgttc gctgcaacaa gccgatggtc gcggccgtga gcggggtcgc ggtcggcgtc
       61 ggcaccacca tgctgttcca ctgcgatttt gtggtcgcgg gctcggacgc atacttttca
      121 acgccgttcg tcagcctcgg cctgatcccg gaagcggcct cgagcctgct ggcgccgcgc
      181 ctaatgggcc agcgccgcgc gttcgagttt ctagtcatgg ggcacccgct taacgcgacg
      241 cacgccaagg agttcggcat cgctaacatc gtagtcgcgc ctgaggacgt cgatgccgaa
      301 ggcttgaagg cggcgcgtga gatcgcagcg ctgccggccg gcgcggtcgc actttcgcgc
      361 gctctgatgc gcggagcgac cgatgagttg ctggcgcgca tcgatcagga agccaaaatc
      421 ttcagcgaac gcctaaattc gccggaggcg aaggcggcct tcgaagcatt ctttgcacgc
      481 aagcggtgac gtcgatgtcg ctcaaaggca agaccctcct catcaccggc gcttcgcggg
      541 gcatcgggct cgcgatcgcg ctgcgcgccg cccgcgacgg cgccaatatc gcgatcgcgg
      601 ccaagaccga gccgacagcc cgagcacacg tctgtcggtg agcgcctggt tgtcgaacct
      661 ccatgcggga attaggtatt gcatcgactt ggcgtcgtcg cgtgcactca gcgcttggtc
      721 cttgtagagc cgtgcgttgc ctcgatttgc gccatgtcga tctcgacacc gagacccggc
      781 cggtcaggaa cggcaattgc accgccgacg atcttcaagg gctccttggt caagcgctga
      841 ccgtcctgtc gtgaggtcga acgcgggcac gcagccatac cattgccgaa gtgacccttg
      901 tgggagttta tcagcgatga cgggtctgca gttctgcacg ctcgcgttta cgaggctaca
      961 cggcgagtgg ggccgtttct taccgttatc ggctccaacg gaacgggcga gggaacacca
     1021 cgcccggcgg gtgacttgaa gcagcataaa cttacaatgg cagggtggag tggtgaagaa
     1081 tctgttcgcg tctttgaccg tcgtgcgtca tgcgtgcgtc ctccgcaggt gattcatgcc
     1141 aaccgccgac tcgtcatccg cttccaaaga ggctgtcacg gccgcgctcc tggtgatcgg
     1201 tggcaagatc tgtctggccg caccaaggac cagagcatcg gctatatatc gcggaatatc
     1261 tgacggctgt cggcatcgat ctcaaggaag tgcgggtcgc cggcgatgag gagaaagcga
     1321 tcatcgatgc actcaatgcg ctccggcatc gctgcaccta tgtcttcacc accggcggga
     1381 tcgggccgac ccatggtgac atcagcacaa tctcagaatt gccgatcagt tcgaactacg
     1441 ggaccagagg gtgagtggac ccccattgct caccatggca tcctcagcgc caatactggc
     1501 agaagagatg tgggttgcat ggcaaaattt cgtagcatct tgcagacggt gggcaacacg
     1561 cctgtcgtca gaatcaacaa tttggcgccg cgcggcgtaa acttgtacgt caagatcgag
     1621 gccttcaatc ccttaggctc cgttaaggat cgcttggcgc tgggtgtgat cgaggctgcg
     1681 gagcagtcgg ggcaacttaa gccgggacaa accgtcatcg aggcgaccag tggcaacacc
     1741 ggtatcgggc tcgccatggt gtgcgcgcag aaaggctatc cgctggtggt cactatggcg
     1801 gagcagttca gcgtcgagcg gcgcaagctt atgcgtttcc tcggggctaa ggtcgtgctc
     1861 acgccggcag tagggcgcgc gtgggctatg gtgaccaagg ccgtcgagct cgccagcacc
     1921 catggttggt tcctgacccg acagtttgag aacgaggcga atgccgattt ccattcccgg
     1981 actaccgccc gcgagatcat agcggatttc aagggtgaaa agcttgacta ctgggtgacg
     2041 ggatacggca ctggcggaac gctgaagggc gtgtcacggg tgcttgcgaa agagatgccc
     2101 gacacaaaga tcatcgtctg cgagcccgaa gacgcgcagc ttctagccag cggagttgag
     2161 caggcacgga atgccgatgg gtctcctgcc tcgcctcatc cagccttcaa accgcatccc
     2221 atgcaaggct ggacacctga tttcattccg aagctgaccg ccgatgccgt aaaaatggga
     2281 gttattgacc gcgttctcgc cataaagggc tccgatgcca tcctctacgt ggggagctgg
     2341 cgcgcaggga aggcatcttc gtgggtatca ccgctggagc aaccttcgcc ggcgccttgc
     2401 acatgtgcgg tgaggcgcca gagaatggga acatcctttg catgctgccc gataccggtg
     2461 agcgctatct gagtacacca ctgttcgcca gcgtaccggc cgatatgact caagaagagc
     2521 tggaaatctc tcgatcaacg ccaagcgcgc agttgccctc gtgatagcct aatctggagt
     2581 gcggcacccg tcgggttact ttgtcgtgcg acctcggagg ggcgaggctt tatcgatggg
     2641 tttggttttg cttgcggctc gccgcccgcc ttacgtgcct ccgccgcagg catcgacgcc
     2701 gacagggcca atgcggcgcc tatcacgatc atcatcattg tcttgactct gccgcatgct
     2761 ccgcaaactt accggttccc aaccctaccg ggcatgtaca gcatctggaa ccgaaggaat
     2821 tgccgaaaac tgcattccca cgctggcttt agcgaagcac atgggcggcg accttcatga
     2881 tgcggccgac cacaacacgg tcggccagca catcgtagtc gtcgtcgctc cactcgccgg
     2941 acagccggct cgctaaggcg cgcttgaggg agagatggcc gtcattgtgc ggaacctttt
     3001 gccagccgtt cttcggcgca gggggcgccc gccctcgacc gctcggacga gcggcacagg
     3061 ggatttgatg taggtcatag ttctgacgag agccacggaa cacattgtcg ttgaaccgca
     3121 attatcgact cttgttcgtc gccggagcga gcaaagatgg caaccaatct acgcatcaat
     3181 ggtgagcgtc tatgctggcg tcttgaccgt ctggctcaga tcggccggat cgccggtgat
     3241 ggggtgtgtc ggctcgcgtt gagcaacgag gacaagcaag gccgtgatct ggtcgtcggg
     3301 tggatgcgcg aactgggctt gtcggtgcgt gtcgataaaa tcggcaatgt tatcgggacg
     3361 cgacgcggac tcgaggatgg acctccggtc atgaccggta gtcatatcga ctcagtgcgc
     3421 actgggggtc ggtatgatgg tgtccttggc gtgctcgcgg gcctggaggt gatcgcgacc
     3481 ttaaatgacg ccgaattcca ctcccggcgc ccgatcgccg tggccttctt taccaacgag
     3541 gagggctcgc gcttccaacc cgacatgttc ggcagcctgg tctacacagg cggtctgaaa
     3601 ttggatcagg cggtcgcgac gagaggcatc gacggcgtat ccgtaggtga ggagcttgat
     3661 cgtattggct attccgggcc cgagccagtc ggtcggccgc aggcgcatgc ctatgtcgag
     3721 ctgcatgtgg aacaaggccc ggtgttggag gcggaaaact tccggatcgg cgccgtcgtg
     3781 ggcgtgcagg gcatttcgtg gacggaattc acgctgtccg ggcaatcgaa tcacgccggc
     3841 accacaccca tgcgcatgcg gcgggacgcc ggctacgtcg ccgcccaaat tgccgtgttt
     3901 gcccgcagct tggccaagga gattggcggg aatcaggtcg caaccgtggg gcttatgacg
     3961 ttgtcgccca acctggtcaa tgtgattgct aacgaggttg tgatgacggt cgatttgcgc
     4021 aacaccgatg acgagcagct acgggaagcc gaacggcgat tgttcgcttt cgccgacaag
     4081 gctgccgccg ccgagaaggt cggcatcgcg cggcgctcgc tggcccgctt cgcgccggtc
     4141 gcattcgcgc cgcatctggt ggagcgtgtc gaggcgattg ctgctgcgtt cggcttcccc
     4201 gtcaggcgtc tgccgagtgg cgcaggacat gatgcgcaaa tcatggccgc ggtctgtccc
     4261 tcctgtatga tcttcgtccc gagcgtgggc ggcatcagcc acaatgtcga ggaatatacc
     4321 gccccggccg atctcgtcgc cggggcggac gtgctgcttc ggctcgtcct cgaattggcc
     4381 gagtaatgcg tcaatacatt cacaatgcga cgatcgtgaa cggcgacggc tacactccgc
     4441 cgtttctcgg tgacgttctg attgagaatg atcgcatcgt cagactcggc gacgtccctc
     4501 cttccgatgt caacgccgcc gaccgcaaac tggacgccac cggcagaatc ctggcgcctg
     4561 gcttcgtcga tactcacaat cacggcgcgt tgggcggcac gatcatcggg gctagcggaa
     4621 ttccacgaag ctgcgagctg gccatacttg caggcgtaac gaagcgcatc tgtggcgtag
     4681 atggattatc gccggcgccg gttgtcgaga gtgatcgatc ccaatatgcg gcccaattaa
     4741 agcctctgga tggcgcaatc gacggcgagt ggagctggag cacggtagcc gaattcttgg
     4801 cgtggcatcg gggccgttcg gtcaccgaca tgggcatgta tctaggacat tgcgccgtgc
     4861 gtcgggtggt catggaaaat ttggcccgtg ttgcgaccga tgcggagatt gccgcgatgc
     4921 aggaggtcgt gcggcgcgaa gctgaactga cgctcgggct ttccaccggc ctggtctaca
     4981 atcctgctgt ctattgcgac caacgcgaaa tcacggcgct ggtccgcgcc tttaatggcg
     5041 tcaagccagg tgcgctgttt ccgcatctgc gttccgagag cgacaacatc gttgccagcc
     5101 tcaaagaagt gctggaggcg gcgatcgatg gcggtggagg ttattgcaac gagcactcga
     5161 agatcgctgg cgagcgtaat tatgggaaat tcgggcaact ggagagcatg cttgacgatg
     5221 ccagatcgct gattcccacc atggagaaca tgtatcccta cgccgccgga tcgactaccg
     5281 gcgatgcgat ttttccgccg gaggcgcgcg ccggctcgcg cgccacgttc ttggcgcggc
     5341 tgcgcgagcc gactgtccgg cgcgcgatgg cggacaagat gcggcacgac accacatcct
     5401 gggataactt ggtcagcttc tgcggcgggt tgcagggtat acagatcgcc ggggtcaaac
     5461 cgaacgtcgg agcgcgctat ctgggcaagc gcctctcgga cgtcgcgttt gaggccggtg
     5521 ctcccgattc cgacagcgat gcggcctacg atgctgtttt cgacttcttt gcggacaatg
     5581 atggcgacat cgccatcatc acgcattatg gcaacgaggc aaccgtcgag cgcttctttc
     5641 gtcggccgaa catggcgatc tgtaccgacg gactcatgcc cggtccgggg caaaaactgc
     5701 atccgcgctc gctcggcgct tttccaaaag ccttacgcat ggggcgggat atgggaatcc
     5761 cattaacgca gatgatttgg cggctttcga ccctcccctg ccgcttctta aacttgcctg
     5821 atcccacgct gaaaattggc tccgacgcct ctctcgtcct atttgatccg aacaccgtcc
     5881 gtgagcgcaa tgactatttg gatcccttgg tatcccccga cggcattgac ttcgtttggg
     5941 tacacgggaa actcgtattg gagcttggta agttgctcgc gccgcagcga tttccgggcc
     6001 gcattctcac cagtcaacta aaggccgcgt gaggacgtca gacgctgttc tcgtaaaccg
     6061 ccccgaacgg cggtcgcgga agagaccagg acaagtcctt tggccgcgat cccaacaatc
     6121 tatccgtcgc ggcacaagag ctcggcgcag cagcgcgtcc ttgtacgtta cgatcgtgct
     6181 gcctggataa ggttggtcct catggagttg cccgcctcca gggaggtgcc ggctcaaagt
     6241 ttacggccgg aggaagtagg ctcaaggtaa cgacagccat gagccccata agagcttcct
     6301 ttctgttcaa tttgcacaaa cattcagcac gcaattttga ttcagcgcaa agatgtaatt
     6361 cttctcccta atatccttac gctggagata tgagtcattc gggtttggcc gggcgatatc
     6421 aggagcttga acatggaatc gaatcaaaag cctacggttg cgcccgtgga cgaaattttg
     6481 ccgttgccga aacttgccgc cctcggcttt caacacgtgc tggtgatgta cgccggtgct
     6541 gtggcggtgc cgctgatcat cggacgtgcg ctgaagctct cgcctgagca ggttgcactg
     6601 ttgatcaatg ccgacctttt cgcttgcggc atcgtgacct tgatccagtc tttcggagcc
     6661 ggagcttggt tcggcattcg ccttccggtg atgatgggcg tgacgttcgc agcggtcggt
     6721 ccgatgctgg cgatggccaa cgatccgagc ctcgggattc tcggaatctt tggcgccgtc
     6781 atagcagccg gcgtgtttac catcattgtt gcgcctgcga tcagctattt cctgccattc
     6841 tttcctccgg tcgtaaccgg ctcgatcatt ctcatgatcg ggatttccct gatgcgcatc
     6901 ggtgtgaact gggccgccgg cgcgtcggtg ccgaccgcgc cgaactacgg cgatccgttc
     6961 aatcttggca tggccgcgtt cgtgcttgtg tgcatccttt tatgcacacg ctacgcgacc
     7021 ggcttcgtcc gcaacattgc ggtgctcatc ggtctgattg ggggttgcat cgtcgcctat
     7081 ctgttgggca agatgaactt cggcaaggta atcgatgcgc cgtggttctc actcatctac
     7141 ccgctgcagt tcggcatgcc gaaattcgaa atcgtgtcga tcatcaccat gtgcatcgtg
     7201 atgatcgtgg tcatgatcga atcggcgggc atgttcctgg cgcttagcga catgaccggc
     7261 aaaagacttg aagccatcga tatgacgcgc gggttgcgga ccgatgggct cggaacattg
     7321 atcggcggtc tgttcaacac gtttccttac acgtcgttct cccagaacgt cggtctcgtc
     7381 ggcgtcaccg gaatcaaaag ccgattcgta tgcgtgaccg gcggaatcat cctgctggcc
     7441 atgggcctcg taccgaaact tggtgcgctg gttgaagcgg tgccgctctt cgtgctcggc
     7501 ggcgcgggaa tcgtcatgtt cggcatggtg gcggcgaccg gaatccgcat tctttcggga
     7561 gtggattacc agacgacccg gaacaatctc tatgtcgtgg cgatctccct cgggttcggg
     7621 atgatcccgc tcgtggcgcc gacatttttc gccaagttac cggcgcagct cgggccgatc
     7681 ttgaactcgg gcattctgct caccgccatc gccgcggtgc tactgaattt ctatttcaac
     7741 ggagtgagtg accgcacgac ggccgtaagt gcggcgcgga gtgcggccac gttgagtgaa
     7801 gcatgacccg ggatctgtct cgggtgagcc ctcggagcta agcctgttgg cttcgatgct
     7861 gaagcgatga tgaatctttc cggtgaaggg gaagatgcgc cgcccaaggc gagctcaagg
     7921 ggcgttcagg acaagcggaa tgagactcat gccggaggtc caagtcactt ttgttgagga
     7981 gcccgcgccg aaaggtctgt acgtagccaa ggatgtgggt gaaatcggcg tcgttcctaa
     8041 ggcaggagcg gtgggcgcgg cattgcgggc ttttgacggc aaacgacgaa cgacgctgcg
     8101 gaggaaaggt tcctcagccg cacgcaccgt aaatgtgggc cgcatcgctg atgcccgatc
     8161 gtgagcaatg gcattgaaca aggcgatctt aatcggcccg gacaggaacg ggcgctgtgt
     8221 ggcggtggag aatgggcttg tagcggcact gcctccgaaa aacgcccgcg tgctgtcgtg
     8281 tgccggtggc gaaatcaggg ccggcgcggt ctgcgcccat acccacctct acagcggtct
     8341 cagtcggtat ggcatgccgg ccgcatcacc gccgccgggg aattttctgc aactgctcga
     8401 gcgggtgtgg tggcggttgg accgtgcgct tgatgaaacc acattgcgag ctgcggcgat
     8461 ggattacgtc gcgcgcgccc tgctcgctgg caccaccgcg ttggttgatc accacgagtc
     8521 accgaaccta atcgaaggct cgctggccat tctggctgaa gtctgtgagc agctcggcat
     8581 gcgggcgctg ctatgctacg gcgccaccga gcgcaacgcc ggccgcgatg aagcaaggcg
     8641 gggcttggcg gaatgccgcc gcgcccctgc ttcgccgctc gttcgcggat tggttggatt
     8701 gcacgcgagt tttaccgttt cgaacgacac tgtgcgcgct tgcggagagc ttgcgcgcga
     8761 actcggcacg gtactgcacg tgcatgtcgc cgaagacagc gccgacgtgg aggacgcgcg
     8821 gtcccgcggg ttcgctggtc ccctggagcg cctttccaaa ctcggagcgc tcgtgcccgg
     8881 ttcgattctg gcacacggcg tccacttgaa tcgcgagcag gcggaaatgg cgggctcgct
     8941 ttcgtgctgg ctggtgcaga atcctcgctc aaacgaagcc aaccgcgtcg gttatgcaag
     9001 ctgcctgcag tacgcaagca aggtggcgtt gggcagcgac ggctggaacg gcgacatggc
     9061 ggaggaacag gatgcgctgt ttcgactcgc cgaactgcac ggcgatcgcc gcgcggatgg
     9121 ccgcttggcg gccggtcatg ctctcatcgc tgaacgattt ggaagcgagc cggaaccgct
     9181 gtcgcagggg tcccttggcg atgtcgtggt atgggaaaac ggacgcgtcc gtcacagcgt
     9241 agtcggagga cgggtcgtgg tggaggacgg tgagctgacc gggagcgcca tcgacgaaat
     9301 atcggccgtt gcgcaaagtt ccgcagtacg tctgtgggat cgcatgcgga tgatgtgacg
     9361 aggcgaagga gagagccgca tggccagcca cggtgcttga gttgcgaaag ctgggtgttt
     9421 gagcatgcgc tttcgttgtc atggctgtag tgcggtgatc gatgcggttc acgcgctgcc
     9481 gttccgatgt cccggtcatt tgccgggaga tgacatcgat cacgtgctgg cgccggacgc
     9541 gatcgatggg gtgtttcccg ccggcacgga gcacaatccc ttcttacgat atcgtacact
     9601 gctctctccc taccgcctcg cgtgcgccgc tggtcttagc gatgccggtt ggacggatat
     9661 cgtcgaaacg ctcgatgcag ccgtgctcgg gatagccggg ggtggttttc gcatcacccc
     9721 gatgaccgaa cagagccgct tggcagcagc gttgggattt agggacgggc tgtgggtcaa
     9781 ggacgagacc ggcgacgtcg caggttcgca caaggctcgg catcttatgg gcgtcatgct
     9841 tcacctgcgt gtgctcgaat tggcgaaatt gccagctggc aaagatctgc gcgcgcgccg
     9901 cttggccatc gcgtcgtgcg gcaatgcggc actcgccgcc gctgtgatag cacgtgcagc
     9961 cgattggccg ctcgatgtgt tcattccgcc gggtgctgac tcagcagtga aacagaagct
    10021 gatagagctt ggcgccaaca tcacgacgtg ccagcgaggt catggcgggg caggcgatcc
    10081 ctgcttcctg cactttcgcg aagccgtagc cgccggagcg attccgttca gcgtgcaagg
    10141 aaccgaaaac ggtctcgcgg tggaaggcgg ccgtacactc gccttcgaaa tgatcgagca
    10201 atttcgcgga gcaggcatcg gtcctgatca gttgttcgtg caagtggggg gcggcgcatt
    10261 ggcgagcgcg ctggcgcaag gctttgcgct cgggcttcga tttggaatgc tggcgaagct
    10321 tcccaggttg atagccgtgc aaacgcatgc ctgcgcgccg cttgcgcgtg catggagaaa
    10381 agtcgatggc ctcgatcttg cgagcgtggc gcgtcatcgt tcgcatttca tgtggccatg
    10441 ggagggcacg accgtgagcc tggccagcgg catcgttgat gatgaaacct atgactggtg
    10501 ggcggtggta gaagggatgc gggggagcgg cgggcggccc gtgctcgtgg atgagacgat
    10561 gatcgcccgt gcacatgaga tttgtcgcct acacactggg atttctgcct cgccgaccgg
    10621 tacggccggg cttgccggcg ccatggctct gccggccgaa aaggggcgca cagcggtcat
    10681 tttgtcagga gttgagcgat gatcagacgt atagtgggac ggcatgacaa actatgctga
    10741 accccatgat atgaccgcgt tttatggcaa gcgttccgca tgtggcgggc gcaatcagac
    10801 aaggtccttt ggcttccgat gctacacctc gcgcgcgtat gtcagagaca cgaccgcgga
    10861 tacgcagatc cgcggcagga cgggtaccgc attgcggagg ctcattcgtg aagcgcgcgg
    10921 gtcttcaggg tgtacgaact gagcaccttc atgtagtttc cgcgttcata cgcggttgca
    10981 ttgggaacag cgtcatagct catgctcccg cacatctcgg caatcgaaac gtattcatgc
    11041 gcgtcgagcc accttgcgag atcagagagc acctgactgg cgtggcgaat accgttcttg
    11101 aggagggcag acgccatgaa tgccacccgt gccccggcca tgacacattt gactacgtcc
    11161 tccgcgctgt gcacgcctcc ggtaattccg agatcagcgc gaacgtggcc atagaggatc
    11221 gccgtccagt gcaaacgtac cagcaattct tcggaacggc tcaatgacag cgtgggaact
    11281 acttccagat catcgatgtt gaagtccggc tgatagaagc ggttgaacag caccaaggcg
    11341 ttggcacccg cttgatccag ccgcagcccc atgttggcgg gcgcgctaaa aaacggcgaa
    11401 agcttaaccg ccatggggat gcgcaagctg cgccgaattt cggtgacgag ctcgcgctga
    11461 tccctttcca cgtcctctcc gggttgcagc ggatcggtcg cgatcgcata aatgttcagt
    11521 tcgacggcat ccgcgccggc ctcttccata agccgggcat attgggtcca gccgcccgga
    11581 gtgacgccgt tgaggctggc tattaccgga atatcaacgc gctccttcgc gcgacggatc
    11641 agttcgaggt accggtcggg cccgagattg taggtctgca tatcgggaaa gaagctgagc
    11701 gcttcggcga agctctctga gcctcgcgcc aaatcatgat cgacgcttcg gctctcgagc
    11761 tcaagttgct cttcaaacag cgacggcagc accacggcgg caataccgtg gtcctcaagc
    11821 ctcacgatat tctccacgga atcagcgagc ggcgacgatg aagcaacgag cggcgaccgc
    11881 aaggtgaggc cgagatacct tgtcgtcaga tcggtcataa gctcctgctt tccgtcgccg
    11941 gcagtgcgct gacctgggat tcgctgggcg cattgtcagc tggcgcgaca tggcccgcca
    12001 tcgccgcgcg attcgcataa atgcgccatt ggcgctcaac gtcttgctgt gccctcacca
    12061 acagcgcgct cgcggcctcc ggattgctcc gcgccagcat ggtataccgt gcctcctgat
    12121 acgcgtagtc cctgaaagct atgcttggcg gcatggaatc gagctggaac gggtttttgc
    12181 catcctgctg cagacggggg tcgtagcgca tgagcggcca gtaccccgac aacacggctg
    12241 acctctgctg agcaagaccg aacgacatgt cgtacccgtg tgcaatgcaa tggctatagg
    12301 cgattatgat cgatggacca tcatatgatt cggcctccag gaatgtcctt acggcatgga
    12361 tgtcgttacc gcccattgcg acccgtgcca catataccga gccgtaattg accgcctcca
    12421 tggcgagatc tttcttcgcg gtatgtttgc cgccggtgga gaacttcgcg atggcgccgc
    12481 ggggcgttgc cttagacatc tggcccccgg tgttggaata gacctccgta tcgagcacca
    12541 gcacattgac gttgcgaccc gactcgagaa cgtgatcgag accgccagcg ccgatatcgt
    12601 aagcccagcc gtcaccccca actagccaca cgctgcgacg caccaatgcg tcggcaacga
    12661 cccgtagcgc cgcagcgtcg gaggaaccca tggtcacaag cttccgcttc aactccgcca
    12721 cccgccgtcg ttgctcgtcg attccggcct ctgtcgactg atcagcctgc agaatcccgg
    12781 tagcaagcgc atcacccagg tcagcactta ggcgcgcgac catttcacgg gcgaactccg
    12841 tctgcttgtc gactgcgagc ctcatgccca gcccgaattc cgcattgtct tcaaacaggg
    12901 agttcgacca tgcgggaccg cgaccctgcg cattcgccgt ataaggcgta gtcggaagat
    12961 tacccccgta gatcgaggag cagccggtcg cattcgcgat caacaaacgg tcgccgaaca
    13021 gctgcgtcag cagcttgatg tacggagtct ccccgcaacc agcgcacgct ccagagaact
    13081 caaacaacgg ttccagcacc tgtacgttct tcacggtgtc gtgcgacaag cggccgcggt
    13141 cgagttccgg taacgacaag aagaattccc aattggtgct ctccgtatcg cagagtggcg
    13201 cctgcggctc catattgatc gccttgtgct ttgcctcact cttgctcctc acgggacaag
    13261 cttccacaca caggttgcag cctgtacaat cttgtggcga tacctgcagc gtgtaacgca
    13321 tggactcgaa ctcgcgccac tttggcttgg ctgctttaaa gctcgatggc gcacgggcga
    13381 tgtgcgaagc atcatagatc ttggcacgaa tcaccgagtg cggacacacc agtacgcact
    13441 ttccgcactg gatgcacagg tccagatccc acaccggaat ctgttgtgcg atgttccgct
    13501 tctcccactt tgccgtgcca gtcgggaacg tcccgtcaac tggcattgcg cttacaggca
    13561 gtgtgtcgcc ccttccggcg attgcttttc cgagaacgtt tttcacgaac tcgggggccg
    13621 ccggcgccac tggaatcggc atatcgaacg aaccgcttgg ttgatgaggc aatggcaatg
    13681 gcacctcgtg caagcgcgtc agcgttcggt cgacggcagc aaaattcttc tgcaccgcgg
    13741 cactgccgcg cttcccgtag gtgtcttcga tcgcctcttt gatcgccgcg atcgcctcat
    13801 cgcgaggaag cacaccgcta agtgcaaaga agcaggtctg catgatcgtg ttgatccgcg
    13861 tgccgatccc gcactcccga gcgattgcga aggcatcgat cacgtagaac ttcagcttct
    13921 tgcgaaggat cgtatcttgc acgtgacctg gaagatggtc ccagatttca tcggccccga
    13981 acggcgcgtt gagcaggaat gtggctccgg gctccgctgc gaggagaaca tcgattcgct
    14041 tgaggaactg gaactggtgg caagcaacaa aattggcttt gccgatcaga tatgtcgatt
    14101 ttatgggatg cagtccaaat cgcaggtgcg atactgtcac cgctccagcc ttcttcgaat
    14161 cgtagacgaa ataaccttgc acgtaatttc ttgtctgttt cccgatgatc ttaatcgaat
    14221 ttttgtttgc cccgatggtc ccgtcagaac ccagcccgaa gaacaccgcc cgaacggtcc
    14281 gcggatgctc cgtcgagaat tctgtgtcgc agtcgaggct ggtgtgcgta atgtcgtcga
    14341 gaatgccgac cgtgaagtgg cgtttcgggt gtgcctttgc gagttcatcg aaaaccgcct
    14401 tcaccatcgc gggtgtgaat tccttcgaag cgagcccgta gcgcccgcca atcacccgct
    14461 gcatcgtcgc gccagcacct gacccatggc gctccataag cgcggtgatg acatcctggt
    14521 acagcggttc cccgatgctt cccggctcct tggtgcggtc cagcaccgcg atcgatctca
    14581 ctgtagctgg aagcgccgca agaaaattct caatcgcaaa cggccggaac agccgaacct
    14641 tcacaatgcc cactctttca ccgcgctcct gcagcgcgtc gacgacctct tctgcggcac
    14701 ccgccccgga tcccatcagc accaggaccc gatccgcatc cggcgcgcca tagtagtcga
    14761 ataagcggta tgcgcgcccg gtcctgcggg cgaggcggtc catcacatcc tggacgatcg
    14821 tgggacagtc gcggtagtag gggttgctag cctcgcgtgc ctggaaaaac atgtcgggat
    14881 tgtgtgccgt cccgcgcagc actggccgat ccggcgacat agcacgctcg cgatgctgca
    14941 ccacgaacgc gttgtcgatg atcgcccgca aatcatcatc gctcagtgac tcgatcgtgg
    15001 cgatttcatg cgacgtccga aagccgtcga agaagtgcag gaatggcacg cgcgactcca
    15061 acgtcgccgc ctgcgcaacc aaagcaaaat cacacgcctc ctgtactgaa ttcgcggcca
    15121 gcagcgcaaa acccgtggtc cgagccgcca tgacatcgct gtggtcgcca aaaatcgaaa
    15181 gagcgtgcgt cgcgatcgtg cgagctgcta catgaatgac ggccgccgtc agctcgccgg
    15241 caatcttgta catgttcgga atcatcagca gcaggccttg cgatgccgtg aacgttgtag
    15301 cgagcgcccc gccctgcaga gcgccgtgca atgcgcccgc tgcgccaccc tcgctctgca
    15361 tttcgatcac atcaggaacg gtgccccaaa tgttggtggc ccccccagcc ttccactggt
    15421 ccgccaattc acccatggtc gaagacggtg taatcggata gattgcgatc acttcgttga
    15481 ggcgatacgc cacggaggcg acggcttcat tgccgtccac aacggcacga gcctgggcca
    15541 tccctcgcct ccttagcgct gcgttatcaa ggccttgcct gcagaatagg caataagagc
    15601 aatcgctagg catttcctca ggattgcatc tcttgccgag tcagttcgca ggtgactgca
    15661 gatgtgcaaa tatattgcca attgtagaaa ggatacaaag gatataaaca cgcgaaaaga
    15721 gctaagcgta acgaccaagg caaatgcggt gcgcgcaatc tgaggccgtt caattgcgtg
    15781 gacgggatgc aggatgcggg cattggagat gagatcatgg cgtaccagat cgtcgcctcg
    15841 ttgtgcacca gctgtggcgc ctgcgaattg gaatgtccga attcggcaat cagcatcggg
    15901 cgcttcacgt atgtgatcga tcccaacaaa tgcaccgagt gcaagggatt ctacgaccag
    15961 ccgcattgcg ccacggtctg cccggtgccg aacacgtgtg taccggccag cccccaaggc
    16021 aactgaacgc acgcagttgt atcgttgatg tatccccacc gctcgactac cattcgacgc
    16081 cagaaaagca cgatcactcg cgcttgatcg tgcaagattt ctgccagcac gcttgcgagg
    16141 ctaacggagc cggtcaccag tgcgttgatg ccggtgcacg gcccagcgta tgcgcccgga
    16201 tgacggcgcc atcgtaagcg acggcgcggc atcgctgtcg tgcattgatt tgtcacatcg
    16261 ttcagactgg ggccgaagat gagcacggca ctcaatgcca ctggtctcgc tcgccatcgg
    16321 gaatgtggcc gagattgatg gcgcgggcgc ttggcgaatc cttcatcggc aggcgagtac
    16381 gccgcacggc gtcaaacgct tccagcgcac cagccacagc gcctgccgtc gggacaaggc
    16441 cgatctctcc aacgcccttc gcgccgaatg ggccctccgg ttcagtatcc tcgaccagga
    16501 tgaccttaac ctccggcatg tgctgggcac gcaagacacc gatatcacga agcttgaagg
    16561 tcacgggcat gccatgcttg caagggagtt cctcggtcag cgcgtagccc agccccatat
    16621 gcaccgctcc ttcgatctga gcctcgcatt gttgtgggtt caaggcgcgg ccgacgtcgt
    16681 gagcggcaac cacccgttcg acagcaccgt tgccactgag caccaccacc tgggtggccc
    16741 agccaaaggt tgtatgggtc ttgatcttgc cgttcttcat ctcgccggga gcagtggtgt
    16801 cgtcgatccg ggtctcgcct gcgtagatac ggccagcaag gtcggcgagc gtcagtccac
    16861 ggttcaggtc ggccttgagt ttaatcgctg catcgaccac cgcccggccg cctaggaggc
    16921 tcgcgcgtga gcccgttgtc tggccggcgc ccaattcgaa acggctgctt acccgcggac
    16981 ggaatatccc agcgggcagg cccgtcgcct cgacggcgca ctgagtgagg atcgtcagca
    17041 gtccctgacc catttcggtg aagcccgtgt agagttcgac cgtgccgccg ggctgcacca
    17101 ccagtcggca ttttccgtac tcgaccgcac cattgccaag tccgcaattc ttgacgccgc
    17161 aggcgatgcc gacggcacgc ccttgcgccc gggccacgtc gtaggcatct ttcaccgcca
    17221 gcagcgtttt ctcgatgccc accgacttct ccagaatctg tcccgtcgtg aaggcatcac
    17281 cgatgcgcaa gacattgcgc cagcgcatct cccagcgatc gataccagct ttcgcggcaa
    17341 gcagatcgag acagccctcg atggcgaaac tagtttgatt gacgccgaag ccgcgcatcg
    17401 cgccgcaggg ggggttgttg gtgtatgcgg cgaccgcctc gatggcgacc gccggcaccc
    17461 gatagggacc gcaggcatgg cctgcggccc gttccaacac cttgctaccc actgaggcat
    17521 acgcgccgga atcgcctagg atgttcacct tggcggcggt cagcaggccg tacgcatcac
    17581 agcccacagt ataggtcatg gtcaacggat gacgcttggg atgcatgcgg atggattctt
    17641 cgcgattgag cacaatccgc actggccgcc ccgtcatgcg cgccaatagt gcggtttgcg
    17701 cttggatggt catgtcctct ttgccgccga aaccgccccc attaggcatg agctcgacga
    17761 ataggcggtc ctcgggttcg ccgagtacgg acgcgacctg acggcgatcc tcgaagattc
    17821 cttggccctg gctgtagatg tgcaggccgc caccgggcag gggcacagcc acggccgcct
    17881 caggctccag gaaaagatgc tcgatgcgtt gtgtggccca ggtgccgctc accacatgcg
    17941 ccgaggcgtc gagagccgcc tccacgtcac cccgcgcgta gcgcgtgact gacaagacat
    18001 tcccgtgttt ggggttgacc tgaggggccc ccagcttgat cgcttcggcg ggatcgagca
    18061 ccggcggcag cgcttcgtac tccacctcga cgaggtttgc ggcctgccgc gcaatgcgtg
    18121 gggtctcggc ggccacagcg gccaccacgt ccccaacata acggacctct tcgccctcgc
    18181 cgacgaagca cggccaatcc ttgtagatct ggccgaccca gcgatcgccc ggcacatcct
    18241 tagcagtcgc tactttgata acaccttgaa gtgccagtgc cttcgtggtg tcgatccgga
    18301 tgaccctggc gcgcgcgtgc ggagacagca cgacgacgcc atggagcagg ccggcaacat
    18361 cgatatcagc aacgaagggc cgtgtgccca gggcgagttc gccgccttga tagcgtcgca
    18421 gcggttggcc tacgccaccg ctgtcgaccg gtgctggcaa ggcaccgcca cgcttggccg
    18481 tttggatgag ctcgaatgca tcgacgatct tcacataccc cgtgcaacgg cacagatgtc
    18541 cgtccagagc tctggccatc tctgcgcgag acagcgcgct ttgttgatcc gtcaaatact
    18601 tgatgcggag cacgaatcca ggcgtgcaga agccacattg caggccggcg gccgcctgaa
    18661 aagcatctgc atatagccgg cgctcgttgt cagaaacgcc ttcgagcgta agaatggatc
    18721 gaccttcaac ctgcgcaatc cgcaccgaac aagtcacctt gggaagtcca tcaatgagtg
    18781 cgagacagca gccgcactcc ttctgaggtc cacaggctgc cttgacggat ttgatcttga
    18841 agatgtcccg aagcacgtcg accagcggct catcaggcca agcctcgacc tcgcggggtg
    18901 tcccattgag atgaaatttg acgaccttca cggcgtccgt tctatttttt caaaaggctt
    18961 aagcgggcga gcctacgggg gagcatgcga gaatcgtgcc aatgtagttg accgcctttg
    19021 gcgccgtcac ggccctaagg atctggtcca taatatgcag ccacgtcagg tcgacggggc
    19081 cgtcggccgt gccgctgacg gtcgcggccg gctcgctcgg atcaagacgc acgtcgaatc
    19141 cctccccacg gtatcgaacg agcccgttcc caatttccat cgtaaccacg cgcccgccga
    19201 agcggccatg catggcgatg ccgttcacga tcctctgcaa cacgaacccg tcccgatgag
    19261 gagaagctgc ccaggcgtcg atgctgccga aaaagagtgg tttgaccaag tagggaccac
    19321 catcctcggg gcacagcacg tcgcaatggc cgcactcgtt gcagacgtcg gcgaaggtcc
    19381 cgatctgtcg ctgcttagtg aaccgcagcg ctccaacggt ctctgaggtc cagccgtcct
    19441 cactccacgc cagtcgctga atgggcacct cccctgtggg gatgacaaag gtgaagttcg
    19501 cgtcattcgg acaaatcggg atgcatttgt cgcaggtcag gcagtcgaac aggaccaact
    19561 tgctgcctat cttgttcggt ggcgtagagt tctcggcagc gcagtagcgg cgatcggcaa
    19621 gaacgcgctc gccgtagact tctgtattga gcagccgcgc gacggacacc caggtcgcga
    19681 atgcctcgcc cgccgtggtt cgcaggtcgc cgccgacgac gagagcagcg cggcaggcag
    19741 cactcttctc atccgacagg gcgagcctcg cgagcgctgc ctctgcctgg ccgtaagcct
    19801 tcagcacgaa ggtgtcgatg tcggcggcgc ccacctcgtc catgcgcttg atgagatcgc
    19861 cgagataatg ccatccgcgc cgatagccgc catgcttgag cagattggtg cacaccgtga
    19921 tcggcttcag cccaagggct acggtgtcgg cgaaattgat ctcatcaata ccggccgaga
    19981 acgagattgg gaagcgatcc ccgaacctct ttcggaatcg gcgcaccagc gcgattgcaa
    20041 gcggatggag cggtggcccg gaaaggtaca tctcctgttg gctcgcggga aagaagctct
    20101 tgtggttggc gaccacgagc gtgttggaga atttgacgcc gaagccgcgc ccaagtgcgc
    20161 gcgcctctgg actcagacgc tccaccaact cggtgacttg ctgccaattg gcatctttgg
    20221 cgaaagcagc atccggaacc ttgagctcgg tgtagccaat tcgctcatgc agaatggaca
    20281 agagctcgtc acgcccgagc aaggtcggat tgagcttgac catgacgttg ataccgacct
    20341 cggtgagcag aaacctggcg atgctctcga tttcttcggg cggacagccg tgaaaggtcg
    20401 acaatgtcag agtgtcggaa atgcgcgagg taaacggcag ggcgcgcaga tggctgaagg
    20461 cctcggggat ctgcgcgcgc aagcgctcga ccactggagt ggcgtccatc atagtggcca
    20521 gaaaattctg caccttagct gagcggatac ctgccaggtc gtaaccaaca ctcatgtcga
    20581 atatcgtttc gccaaggcct ggggcgagcc ctgaggcctt cagcatctcg atcagcatcg
    20641 agcctttgac gtactcctcc agcgacttat cgacggtcaa ctcctgtgac cactcgacat
    20701 tgaagccgac cgtcgcgaca tcgatacacg gacgggggat ggtcaaatcg tctttcacct
    20761 gaatagtctt caactcgatc acgcggccgc ctgcgagcca ggacaacaca atattctgcg
    20821 ccatctgcgt gtgggggccg gctgccggcc caaacggggt cgatgcacga cgtccgtgaa
    20881 tggcgacgga gagatcgtgc ctgggattgc cctgaacgaa gcgttgagac gggagatcga
    20941 aggccgagcg cttccgttcc agctcgcaaa aaaggcggct gaccagaacg ccaaatggaa
    21001 tgggtaccaa ctgaaccaat attcaacctc atgcagccgg cgacacacca cactcaagcg
    21061 tgtgcgcgcg ggcgactcta tggtgaactc tactatccca cagatgcgac cgcgcattga
    21121 cccggagcag cagcgccttg gacatcagct ccgcccggct ctcggatagt cctcaatccg
    21181 ctggccatcg ggtatcgaaa gtccaaagct ctgatcgtag gtgacggtta ggacgcgacg
    21241 tcattgcgct tgacgcggcg cgcggtagtg aactcggtcc atgtggtgtt ggcgacatgc
    21301 acgccaggga gcggatcgcg gggcacatat ttgccagcgc ccgccctggc ccttagctcg
    21361 ccatcgagcc aactgatctc cccgcgcgac aacgtcgcgt gcggctggcc tgtgcagaaa
    21421 aaaccttcga acacattata gtcgatccgg ctcacctgct ttttggcgga gatcgtcgtc
    21481 gtcgctctgg gatcccaaat gactagatcg gcatccgagc ccacggcgac cgcgcccttt
    21541 ttgggaaaga tgttgagaat gcgcgcagaa ttggcagacg tgacggcgac gaattcctct
    21601 ttcgtgagcc gcccggagtt aacgcccgcc gtccatagca cccgcatccg gtcctcgagc
    21661 ccaccggtgc cattcggtat cttagtaaaa tcgatgcggc cgagccgctt ctgtgcggtt
    21721 gtgaaggtgc aatgatcggt ggccacgacc tgtagggacc cagattggag gccagcccaa
    21781 aggctcgcct gatgatcctt agtccggagc ggcggcgaca tcacgcgctg cgccgcatga
    21841 tcccaatcct cattgcggta ttcgtcctcg tcaagcagga gatgctggat cagggcttca
    21901 ccaaagactc gtttgccggc ggcgcgcgcc cgcgtaattg cgtcatgcgc ctcacggcaa
    21961 gagatgtgaa cgacgtaaag cgggcagccc gctacgtcgg cgatcataat cgcgcggttt
    22021 gcggcctcgc cttcgacgcc aggcggacgc gaataggcgt gaccttcggg gccggtcacg
    22081 ccgtcatcga gataatgctg ctgaaggcgc cagacgacat cgccgttctc ggcgtggact
    22141 agcggtattg cgccgaggct ggcgcagcgc acaaacgagt tgtagagatc gtcgtcattg
    22201 accatcaagg cgcctttata cgccatgtaa tgtttgaacg tgttgatgcc gtaggttttg
    22261 acgcaggtct ccatctcgtc aaagacttgc ttgtcccacc aggtgattgc catgtggaag
    22321 ccgtagtcgc acgctgcgcc ttcggcctcg cgacgtcggt cctgataggc ggcgagcagt
    22381 gtttggccgg ggttaggggt gcagaagtcg acaaccatcg tcgtgccacc cgagagcgcg
    22441 gccttgcaac cccattcaaa attgtcagcc gataccgcac ctccgatgga ccgcaattca
    22501 agatgcgtat gaggatcaat accacctggc atgacatagc atccgccagc atcaatcgtc
    22561 tccgttccgg gcggcgcttc gagacttgcg ccaaccgcag tgatcaggcc atcgcaaata
    22621 agaacgtcgg cgcgctgcga atggtcgtgg ttgacgatgg tgccgccgcg tatgagactt
    22681 gtcatctcta ctttcaggtc ctagcccgca ttctcacact actgttgcac aaaataacac
    22741 cggtcgcacc gtgaatgttg ctcagaacgg gccatggaac gtgttgacgc cgaaaggcgt
    22801 ctgtcgagat ttgctttggc aggcctcgtg gggtcagacg ccggatgttc agaaaaatcc
    22861 tgtaccgatc acgctttcaa tgcgctgcca ttgccacttc agatcgctaa gcatgataag
    22921 gccgagctgc ataccgggcc cgtcagtacc gtgacccgag ggggatcggt caattcgaac
    22981 cgcaccggac gcctcgtctt tttctgacgc atcggccaca ttctggtcac gacaaggcgg
    23041 ccgagtgctt gatcaaggat cgcgacgcgc tgctcgcctt ctacgatttc ccggccgagc
    23101 agtggaagca tctgcgcacc acgaacgtca tcgaaagtgc gttcgccacg gtgcgccatc
    23161 gcaccatgcg gtccaaagga tgtctctcaa acaagaccgc gctcgccatg atcttcaagc
    23221 tcgccgaggc tgccgaaaga aattggcgtc gcctcaatgg ccacaaccag ttgccgaaaa
    23281 tcatcctcgg tataaagttc accgatggaa tcggagtagt cagatcgcaa gctcaaaccg
    23341 ctgccgcctg atcccttccg tcaccaaaat tcggcaatag ctctggagag agcggtttca
    23401 acgtcatcgg cagaacggct cacaccatcg cgcgaagccc ctcatcgcgc aagccggagc
    23461 cgtccgccat gtcgaccccg gaaccgtatt ggggtgttct gcagctgcag gccaatcgca
    23521 caaagctggc gctgcattgc ctcgagctcg ccggctatgc ggtctatcac ccacgcatcc
    23581 gcgagcgccg cgtcgtccgc ggccgctgga ccagcgtgtt gtgtccgctg ttccccggct
    23641 actgcttcct gcacatccat ctgcagtggc acagtgcgcg ctgggcgccg ggtgttgtgc
    23701 gcttggtgct caacggaggc gctccagcgc ggctgtccga gccggcgatt gccgctattc
    23761 ggcagcgcga acgcaacggc ctggtcgaac ttccccggcg cccactgcag cgtggcgaca
    23821 aggtgagggt ccggcgcggt ccgttcgagg ggcattcggc atctatgacg gcatgaccgg
    23881 ccatgagcgc gccgctgtgt gctgcacggc caactggaca ccaacaggtc acaaatctga
    23941 ccgtcgcgtc ataagacatt gatctgaata ttagtttcca ccatcgccga cgtaatacaa
    24001 aagcccttat aggggatgtg ctggaactgg gtgccggtat taagcgccat ccattcgagc
    24061 acgatctggt tgatgctgcc gttgccgggc gtggcgaccg agatgcgccc aggctgcgcc
    24121 ttcgcggcgg caatcacgtc cgcgacgctg ttgtagggcg caccgccatg cgccgcgagc
    24181 acgagcggcg cgtcgctcac catcgcgatg ggtgcaagat cgcgctcgac gtcgtagggc
    24241 atctccttga acatggccgg attgatcgcg acgtccccgc cggttgccat gaccagcgtg
    24301 tagccgtcgg ccgcggcctt tgccgtcgcc gccatggcga tgaaaccgtt gccaccaggc
    24361 cggttctcca ccagcacttg ctggccccag gcttccgtca gcttggcgcc gaggatgcgc
    24421 gaggcgatgt cggagatgcc gccgggggca tagggcacca tgatgtggat gggcttggcc
    24481 gggtagttct gcgcctgcgc gggcgccgtc cagaaaacga cgagcgcgat cacacttgcg
    24541 atccaagcca tgaacacctc ctgcgccgac gcctcggacg cataacctgc atgagagacc
    24601 gccctcgtgg caaggcgcgg cggcgccgca gggcgcaacc gccttcagac ctcgcgcacg
    24661 acggcgctct cgtgccagcg ccgcagcagc agatgggaga cgagcgagag cgcgaagaag
    24721 atcacgatgc cggcggcgga gaggagcagc agcgccgcaa acatgcgcgg gatgttgagc
    24781 cggtagcccg attcgacgat gcgataggcg aggcccgagc cggcgccggc cgaacccgcc
    24841 gcgatctcgg cgaccaccgc gccgatcagc gacagcccgc ccgcgatctt caagccgccc
    24901 aggatatagg gcagcgccgc cggcagcttg agctcccaca acagctggcg ccgcgtggcg
    24961 ccataaaggc gaaaaaggtc ggcgaggttg tgatcgacgg aacgcaagcc gagcgtagta
    25021 tttgcgagca cggggaagaa ggcgacgatc caggcgcagg cgagcaccgc ggcctgctgc
    25081 ggcagataga cgaggagcag cggcgcaatc gccacgatcg gcgtcacctg caggatcacc
    25141 gcgtagggat aaagcgagta ttcgatgaga cgcgactggt tgaacagcac ggcgagcccg
    25201 acgccgccgg cggccgcgag cagaaaacc
//
LOCUS       DATLJT010000340         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_938092,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000340 DATLJT010000000
VERSION     DATLJT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938092"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VM910_07830"
     CDS             <1..1570
                     /locus_tag="VM910_07830"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HVI62483.1"
                     /translation="VLFELSHDYVGDLSETVALMWQARTQAGISPHARDTSTSACARP
                     LERKRADGPQGHDELLLSSVVTALSSLGKSELPAQLARWLDQLDETGRWALLKLVTGG
                     LRIGVSARLAKTAAATLGDKDPQEVELIWPSVRPPYVELFAWLEGRGHKPAAGDAAPF
                     RPAMLAHAIDDSDLGALDPKDFLGEWKWDGIRVQAVTGTGEDGRMIARLYSRTGEDIS
                     GSFPDLIEALRLPGAIDGELLIMRDGRVQSFNVLQQRLNRKTVNAKLLAEFPAHLRAY
                     DLLVDGEEDLRERPFTERRRRLERFIARLAEPRIDLSPLVTFATWDDLAVARKDPAAA
                     GAGADAEAVEGVMLKRRDAAYLPGRPKGPWWKWKRDPFIVDAVLMYAQRGHGKRSSYY
                     SDYTFGVWSKGEDGDVLVPVGKAYFGFTDEELIQIDRFVRRNTVERFGPVREVVHETN
                     QGLVIEVAFEGLQRSTRHKSGLAMRFPRINRLRWDKPPGEAGRLETLERMLEKIETGR
                     GATAAANQGDRMLS"
     gene            1567..1866
                     /locus_tag="VM910_07835"
     CDS             1567..1866
                     /locus_tag="VM910_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HVI62484.1"
                     /translation="MSMPTERPMANNYLNRFFGGPPLSVIFRLILLSILIGVILEVIG
                     LDPWNIIESLKRLVLHIWDMGFDAVRWLWRYLLLGAAVVVPIWLVVRVMRVTKGQ"
     gene            complement(1938..>2278)
                     /locus_tag="VM910_07840"
     CDS             complement(1938..>2278)
                     /locus_tag="VM910_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HVI62485.1"
                     /translation="RDQATAREPGGLSGRPLFPLATRMLAETYVRVEGAFPLIGVGGI
                     DSDQAAVAKIRAGASLIELYSALVFHGLRLVAAIKSELSEGLRRAGQARIDELVGADA
                     AAMTAEKWPT"
BASE COUNT          404 a          721 c          719 g          434 t
ORIGIN      
        1 ggtgctgttc gaattgtccc acgattatgt cggtgacctc tccgagaccg tcgcgctgat
       61 gtggcaagct cgcacgcaag ccggtatatc gccgcacgca cgggatactt ccacctccgc
      121 ttgcgcgcga ccgctggaaa ggaagcgtgc tgacggtccg caaggccatg atgagttgtt
      181 gttgtcttcg gtcgtcaccg cgctctcctc cctcggcaaa tcggagttgc ccgcgcagct
      241 tgcgcgctgg ctcgatcagc tcgacgagac cggccgctgg gcgctgctca agctcgtgac
      301 cggtggcttg cggatcggcg tctcggcacg gcttgcgaag accgcggctg ctacgctcgg
      361 cgacaaagac ccgcaagagg tcgagctgat ttggcccagc gtgcgcccgc cctacgtcga
      421 actgttcgcc tggctggaag ggcgcggcca caagccggcg gccggcgatg ccgctccctt
      481 ccggcccgca atgctggctc atgcaatcga tgatagcgat ctgggggcgc tcgacccgaa
      541 agattttttg ggcgaatgga aatgggatgg cattcgcgtt caagccgtca cgggcacagg
      601 cgaggacggc agaatgattg cccggctcta ttcgcgcacc ggcgaggata tttccggcag
      661 tttccccgac ctgatcgaag ccttgcggct accgggcgca atcgatggcg aactgctgat
      721 catgcgcgac ggccgcgtgc aaagcttcaa cgtgctgcag caacggctca accgcaaaac
      781 agtcaatgcc aagttactgg ccgaatttcc cgcgcatctg cgcgcctacg accttctggt
      841 tgacggtgaa gaagacctgc gcgagcgtcc cttcactgaa cggcgaaggc ggctcgagcg
      901 gttcatcgcc cgcctcgctg agccgcgcat cgatctctca cccctggtga cgttcgccac
      961 ttgggacgat ctcgccgtcg cacgcaaaga ccccgcagcc gcgggtgccg gcgctgacgc
     1021 cgaagcggtc gaaggcgtca tgctcaagcg ccgcgatgcg gcctatttgc ccggccgccc
     1081 caagggcccg tggtggaaat ggaagcgcga tccattcatc gttgatgcgg tgctgatgta
     1141 cgcccagcgc ggccacggca agcgctcgtc ctattattcc gactatactt tcggagtctg
     1201 gagcaagggc gaggacggtg acgtgctggt gcccgttggc aaagcttatt tcggttttac
     1261 cgacgaggaa ctcattcaga tcgatcgctt tgtccgccgc aacaccgtgg agcgcttcgg
     1321 cccggtgcgc gaagtggtgc atgagactaa tcagggccta gtgatcgaag tcgcctttga
     1381 gggtttgcag cgctcgacgc ggcacaagtc aggcttggcc atgcgctttc cgcgcatcaa
     1441 ccggctacgc tgggacaagc cgcccggcga ggccggccgg ctggaaacac tcgaacgaat
     1501 gctggaaaag atcgagaccg gtcgcggcgc gaccgcagcg gcaaatcaag gtgataggat
     1561 gttgtcatga gcatgccgac ggagcggccg atggccaaca attacctcaa tcggttcttt
     1621 ggtgggccgc cgttgtcggt catcttccgg ctgatcctgc tgtcgatcct gatcggggtg
     1681 atcctggagg taatcgggct cgatccctgg aacatcatcg aaagcttgaa acggctcgtg
     1741 ctgcacatct gggacatggg cttcgatgcg gtgcgttggc tctggcgcta tctcctcctc
     1801 ggcgccgccg tggtggtacc gatctggttg gtcgtgcgcg tcatgcgcgt gacaaagggc
     1861 caatgattgt acttacgcgc cgcccggatt tcgggcctga cttcgggagg acatggagga
     1921 accggcgaaa ccgcacctta tgtcggccac ttctccgccg tcatcgcggc agcgtctgcg
     1981 ccgacaagct cgtcgattct cgcctgccca gctcgacgca gcccttccga cagctcggat
     2041 ttgatggccg cgacgagtcg cagcccgtga aacacgagcg cgctgtagag ttcgatcagg
     2101 gacgctcccg ctctgatctt cgcaacagcc gcttggtccg agtcgatgcc gcccacgccg
     2161 atgagcggga acgcaccctc gacccgcaca taggtctccg ccagcatgcg cgtcgcgagc
     2221 gggaacagcg gacgcccaga caggccccct ggctcccgtg ccgtcgcttg atcgcgca
//
LOCUS       DATLJT010000341        11336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3005893,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000341 DATLJT010000000
VERSION     DATLJT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005893"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..711)
                     /locus_tag="VM910_07845"
     CDS             complement(121..711)
                     /locus_tag="VM910_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HVI62486.1"
                     /translation="MGLLDVLNGMQQGPRGPSTPSSQSSGGGMSPMTMAILGLLAWKA
                     VKHLTAGAQPSAAPRSAPSPAPASGTAPAGGTASAGGGGLGDVLKGPLGGLLGGAAAG
                     SVISGGLGDLLKQLQQGGQGDAANSWVGNGPNKAISPGDLASALGVDQIDQLSSQSGM
                     SRNELLDGLSQYLPRVVDHLTPDGRLPTEDELSDRL"
     gene            888..1112
                     /locus_tag="VM910_07850"
     CDS             888..1112
                     /locus_tag="VM910_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62487.1"
                     /translation="MSRFWAVSAFAILLGIAGGTSQASAAPPTVTPSPGYDARLQEQH
                     KAATATPTPADPVAPSPQVRKRHVKRTSSH"
     gene            complement(1157..1714)
                     /locus_tag="VM910_07855"
     CDS             complement(1157..1714)
                     /locus_tag="VM910_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62488.1"
                     /translation="MTCLVALQPVRVCAQWWHRAPADYEECAEAAEKSATSEQKNLAL
                     AECNAKFAGRRKPGGGYTYYDFMQNRSFDIAGPNPTTEEQKRIDEQYAIYLGNQRRSN
                     LAAAVAAKQPEVRETSLKSDVARVPLPRPKPPTNGDARTGQRPAECARPSLSCEWPHL
                     SEAINDLKKKLFGSQPSKTKRDDRS"
     gene            1919..2353
                     /locus_tag="VM910_07860"
     CDS             1919..2353
                     /locus_tag="VM910_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62489.1"
                     /translation="MSYRHTQRGTTILVTCLLVGVLSVGILWGAGQWPQAIVLAVLLG
                     ALAVLFSSLTVEVSDQELRWYFGPGLWTYRVPLDAIKQVTVVRNHWWNGWGIRIGPGL
                     RLYNVSGLDAVELSLGAEDVRRRIGTDDPEGLAAALTARRGA"
     gene            2945..4579
                     /locus_tag="VM910_07865"
     CDS             2945..4579
                     /locus_tag="VM910_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI62490.1"
                     /translation="MPPSALAAESPFDTDTSPAAEELRAGQAHYAGGRIEPAMAFRRG
                     LAARTASSATATIAELHAKLGNAHMLRGELDLAGENYKAALRIAPHLCACWCNLGTVH
                     LKTGKANDAIALYFEALKLNPAHWPSRSNLAEALIATRQHLLAKALLTELAEERPQDG
                     QVRHRLGRACFELNEIEAALEHFKQALAINAQDAESLYWIGGISQKLGQLDAAQAAYA
                     RAAGIQPLIRRAASKSPADFRALALYAPFAGNTPADYLFKDAAYDTDTLALIAGSEPD
                     LIPLGGIDLVVNLISDADQAEAVLPAAARLVAKLGKPVVNDPGKISRTTRDAVAALLP
                     GIPGCRIPKILRLDAGADVSVAALESLLPFPFPVLARPAGTHGGDDFEKIEDLDALSL
                     FLKCRPGDDHYVIEYVAYASPDGHFRKYRFIFVGHEILPYHLAIGNDWKVHHASTDMA
                     NQPWMQREEAAFLADPAAVFKPANYAALRAVCERIGLDYFGIDCALDDRGDVLVFEVN
                     ASMLVHDDNAEFSYKDPAVRAIKSAFDAMLQARAGA"
     gene            4631..5116
                     /locus_tag="VM910_07870"
     CDS             4631..5116
                     /locus_tag="VM910_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62491.1"
                     /translation="MGRARLGLQTFFAAIIALCVVAVESPALARMLLENRDPAQGNTE
                     ADATIAGVWVGTYSYRNDRASVGFTFFFPPATDNSCIGRSEEQNTFGNRSAPKLYAIL
                     SCSETALYPGQTLTIRKTYDGTGGVSHTVMYTGKVSADLRTISGEWVLNDIRGTFTMS
                     R"
     gene            complement(5132..6106)
                     /locus_tag="VM910_07875"
     CDS             complement(5132..6106)
                     /locus_tag="VM910_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVI62492.1"
                     /translation="MDTFRRAKLSALAATALALALPSAASRAEVGEQGKADHVKIELW
                     DGNRAMTLINEQLRFTPRSLDTPGHQQTIDFIKSELAKAGIDDVSTQRWAYRSDDGKS
                     YAMTNIIARLDPSNPRRIIVATHYDSIVRAYADKSNPQGPMPGANNSASGVAVLLETA
                     RVLSLLPKPAVGIDMIFFDGEEGPRSLGAGDPNWKALGSPYFAAHLRDFYPNAKPEKA
                     VVFDMVCDRDLKLRPEPSSVASALPEVQKYWRTGQTVAPSAFDTHMISYPISDDHTAL
                     AAAGIPSFLVIDFEYEPFFNTTQDTADKCSVGSLEAVGRTTLQYLYLP"
     gene            complement(6124..6732)
                     /locus_tag="VM910_07880"
     CDS             complement(6124..6732)
                     /locus_tag="VM910_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVI62493.1"
                     /translation="MTSRFRVLAFTIYVIAFIVGGVLMGFEMLGSRYLFPYFGGGIGT
                     WASLISTVLCALAIGYFAGSAMIAHHPTQQTIGVFILIAAAYLACVPATADGVMEKIL
                     SGLGDGPAATLTASTALLLVPLSLLGTFSPIAVSILTRSAEEAGRVAGLVYGVSTIGN
                     VVGTLLTTFTLIPTIGSRAITYIFALTLACCAGVLFLPFGRR"
     gene            complement(6729..7655)
                     /locus_tag="VM910_07885"
     CDS             complement(6729..7655)
                     /locus_tag="VM910_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVI62494.1"
                     /translation="MVIRSVMLGAIAGALVGVAAAPAQDSRNLLESKESLYNNIYVYE
                     QPPYVSMTFGHNRRIYTESVYNTRDDRDLPVEYTRFMTASLMYAKDVHSVLEIGFGGG
                     RTSWYLHRYLPNVQVTSVELDPTVVELARKYFGIKEEPNFQLVNRDGRLFLQESKEHY
                     DLILIDAYRGPFVPFHLLTKEFYQIVREHLADGGVVAQNVEPSTMLFDSAVKTINAVF
                     PQLDFYRADGNIVTVAYDGAERKPEDLAGVARDRDKAYGLRYSLLEMLSQRRHIQIAG
                     GNVIDPNAKVLTDDFAPVETLKNIERHNRKLP"
     gene            7762..8760
                     /locus_tag="VM910_07890"
     CDS             7762..8760
                     /locus_tag="VM910_07890"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HVI62495.1"
                     /translation="MSAGRPVIWLAAMMMAGTGSVAAEAPASLAPLMEQKQVVASELA
                     RQVAFCSQRNDTDHPAFRGCVDWHSAVHGVWALIAYQRATGDAQYAALVDSILEKEAL
                     AQEQEHLRQAPLFEMPYGRAWFLRLAIDHRKLTGSDDLTAFGNEVALSLRDYYRHRAI
                     DRFSAAYQSASWALINLLDYARDRNLSDLEAEVTGWIKTGFVDVAPACANERERGEFM
                     GICTNWAALVARVLDRDRYSNWLETFIAANGLPAPLARQSGDHEFGLNFSRAWGLWDM
                     YAASGRADVLEAYVAHFNNGFTPASNWSGSYMAVGHWVAQFGLFALQPLFGPEAGR"
     gene            8939..9577
                     /locus_tag="VM910_07895"
     CDS             8939..9577
                     /locus_tag="VM910_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HVI62496.1"
                     /translation="MYQPPAFREDRIEVQHALIRAHPLGLLITAGPAGLLANFFPFLV
                     DAEGDKGRLRLHIARANPQWRELDAIEECLVVFQGPQDYVTPSWYATKRETGKVVPTW
                     NYATVHVWGRPRVVNDDAWLRRQIEDLTASRESLREAPWAVGDAPAEFVAMQMRAIVG
                     VEIPISRIEGKWKMSQNRPEADRRGVIEGFRAMGEAGEEIAALVAERGERLK"
     gene            9775..9966
                     /locus_tag="VM910_07900"
     CDS             9775..9966
                     /locus_tag="VM910_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62497.1"
                     /translation="MGNVYLPAEKGNPVPASRIQDEDDGFDSDMNSDMIKLLEENARL
                     RALVTQLSSLVLKHVVDQK"
     gene            10140..10216
                     /locus_tag="VM910_07905"
     tRNA            10140..10216
                     /locus_tag="VM910_07905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10174..10176,aa:Arg,seq:cct)
     gene            10296..10988
                     /locus_tag="VM910_07910"
     CDS             10296..10988
                     /locus_tag="VM910_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HVI62498.1"
                     /translation="MKDIVAQKLKIDVGTSGTVSALLMRPREARACFVFAHGAGAGIT
                     HPAIETMATGLAERGVATLRYQFPYMERGSKRPDPPGVAHAAVRAAVAEAARRCAGLA
                     LIAGGKSFGGRMTSQAQAKAPLDGVEGLAFLGFPLHPAGQPSTDRAAHLADIHIPMLF
                     LQGANDQLAKVDLLKPVVAGLGRRATLHLVEGADHSFHVPKRSGRDDKEVMAEVLDAF
                     AAWTGVTVAKRG"
     gene            11133..>11336
                     /locus_tag="VM910_07915"
     CDS             11133..>11336
                     /locus_tag="VM910_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430770.1"
                     /note="catalyzes the formation of biotin from
                     dethiobiotin; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HVI62499.1"
                     /translation="MAPPRWTSGTAFSLYMQPFNDLLFRAHAIHREHFDPNRVQLSRL
                     LNIKTGGCPEDCGYCSQSSHHATG"
BASE COUNT         2021 a         3533 c         3654 g         2128 t
ORIGIN      
        1 cccggcgaga gcatccgcgc gatgataccg gccacgaagc ccacaatgat gacccagata
       61 atgccgctca attgatgacc ctccttcaaa catcaccaaa gtctcgcgcc ggcgcgcaac
      121 tcaaagccgg tcggacaatt catcctcggt cggcagccgg ccgtccgggg tcagatggtc
      181 gaccaccctc ggcaggtatt ggctgaggcc gtcgagcaac tcgttgcgcg acattccgct
      241 ttgcgacgag agctggtcga tctggtcgac gcccagcgcg ctggcgaggt cgcccggcga
      301 gatcgccttg ttcggaccat taccgaccca tgaattggcg gcatcgccct ggccgccctg
      361 ctggagctgc ttcaagaggt cgccgagccc accggagatc acgctccccg cagcggctcc
      421 gccaagcagg ccgcccagcg gtcccttcag cacgtcgccc aatcctccgc ctccagccga
      481 agcggtgccg ccagcgggag cggtgccgct agcgggagcg ggcgaggggg ctgaccttgg
      541 tgcggccgac ggctgcgcgc cagcggtcag atgtttgact gccttccatg ccaaaagacc
      601 caggatcgcc atggtcatgg gcgacatccc gccgccgctg ctctgcgagc ttggagtgga
      661 tgggccgcgc gggccttgct gcatgccgtt gagtacgtcg agtaatccca tggatctctc
      721 ctgccgcgtg ccccaccgga aaaagcttaa ctgtccgtca tgacggttac aaggcgaggc
      781 cgtctttgcg ggttcgcggt gagctctcaa gcggcggacg aggctgtcgc ccggcgtctt
      841 gaattcgcat atcatccttc ctcgcacgcc gtggccggag gatcaggatg agccgatttt
      901 gggcagtttc agcattcgca attctgcttg ggatcgccgg cgggacatcg caggcgagcg
      961 cggcgccgcc aacggtgaca ccctcccccg gatatgacgc gcgcctgcag gaacagcaca
     1021 aggcggcgac ggcaacgccc acgcccgccg atccggttgc gccgtctccg caggtgcgca
     1081 agcgacatgt gaagcgcacg tcgtcgcact gatcaggcac gacgggcgcc ggccatcgcc
     1141 gtctgctgat cgaggtctag ctgcggtcgt cgcgcttggt cttgctcggc tgcgagccga
     1201 acagcttctt tttcagatcg ttgatggctt ccgacaaatg cggccactcg caggacaatg
     1261 aggggcgcgc gcattcggcc gggcgctggc ccgttcgggc atcgccattg gtgggcggct
     1321 tggggcgggg cagcggcacc ctggcgacgt cgctcttcag cgaagtctca cgaacttccg
     1381 gctgcttggc cgcaacggcg gcggcgagat tgctgcgtcg ctgatttccc agatagatcg
     1441 catattgctc gtcgatgcgc ttctgctctt ccgtcgtcgg attgggaccg gcgatgtcga
     1501 agctgcggtt ttgcatgaag tcgtaatagg tgtagccgcc gccgggcttt cgccgtccgg
     1561 cgaatttggc gttgcactcg gcgagcgcca agttcttttg ctctgacgtt gcggacttct
     1621 cggcggcctc ggcgcattcc tcgtaatcgg ccggcgcacg atgccaccat tgcgcgcaga
     1681 cacgcaccgg ctgcagtgcg accaaacagg tcagcgcagc gacatgaagc gcggtcaaac
     1741 gcaaggcaat cacagccaat agtccccagc aaatcggcgg cgacgattgc cacgaattgt
     1801 taccattttg cggctttacg cccgggaaga cggcaatttc cccggcataa cgcgtcgaac
     1861 ccggggatag agtccagcga ccaaagggcg gccaacaaca aagtccaggg gtgatgtcgt
     1921 gagctatcgt catacgcaac ggggaaccac gatcctggtc acctgtctcc ttgtcggcgt
     1981 cctcagtgtc ggcatcctgt ggggggccgg gcaatggccg caggcaattg tcctcgcagt
     2041 tctcttgggc gcgcttgcag tgttgttctc ctcgctcacg gtcgaggtga gcgatcagga
     2101 gttgcgctgg tatttcgggc cgggcctttg gacctatcga gtgccgctcg acgccatcaa
     2161 acaggtgacg gtcgtgcgga accactggtg gaacggatgg ggcatccgga tcggacccgg
     2221 cttgaggctc tacaacgtct cgggcctcga tgctgtcgag ctcagcctcg gcgccgagga
     2281 tgtccgccgc cgcatcggca ccgacgaccc cgagggtctt gcggccgcgc tcacagcgcg
     2341 gcgaggggcg taacgcgcgc tgacggccga tcggcagggc ggaaccattg agcggctaaa
     2401 acgcgatgaa ttttgcggga atcgctgcgt ccgcgagccg agtgacctct cccgcttgcg
     2461 ggacctgaaa tccgaccccc accccagccc tccgcctttc agcgggaggg agtacacttc
     2521 cgctgcggtt gaagcgagcg cagcaacgag aagagatcgc gctgcaagtt cggcatggct
     2581 cgagtcgtgg attttcgccg cgacgtgaga ggttgatttt cggtttagtc gtcccacgga
     2641 ctcgtcatgc ccgggcttga cccgggcatc cacgcctttc tttcgatttg ccacgacgtg
     2701 gatggccggg acaagcccgg ccatgacgat gcggaaaggg ccccgccctc taaggtcgca
     2761 aacaggggag ggagcgcacc tccggcgcag caatatcaac ctaatctcat cgcgctttag
     2821 ttgccgcggt gccggcacct caaggcgaaa tgatgcgtcg aattcgcgcg cagattgcac
     2881 gcgctggaag ctttttgttt agcggtagcg gtcatggtta accgaaactt tcgagagccc
     2941 tgccgtgccc ccttcagccc ttgccgccga atctccgttt gataccgaca cctcgcctgc
     3001 cgcagaagaa cttcgcgcgg gtcaggcgca ctatgccggc ggccggatcg agccggcgat
     3061 ggcgtttcgg cgagggcttg ccgctcgcac cgcctcttcg gcgaccgcaa cgatcgccga
     3121 gcttcacgcc aagctcggca acgcccacat gctgcgcggc gaactcgacc tcgccggcga
     3181 gaattacaag gctgcgctaa ggatcgcgcc gcatctttgt gcctgctggt gcaacctcgg
     3241 cacggtgcat ttgaagaccg gcaaggcgaa cgacgcgatc gcgctctatt tcgaggcgct
     3301 caagctcaat ccggcccatt ggccctcgcg cagcaacctg gccgaggcgc tgatcgcaac
     3361 aaggcagcat ctcctcgcga aagccttgct gacggagctt gcggaagagc ggccgcagga
     3421 cggccaggtc cggcatcggc tcggccgcgc ctgtttcgag ctgaacgaga tcgaggcggc
     3481 gctggagcat ttcaaacagg cgctcgcgat caacgcgcag gatgccgaga gcctttactg
     3541 gatcggcggc atctcccaga agcttggaca gcttgacgct gcgcaggcgg cctacgcacg
     3601 ggccgcaggc attcagccgc tgatccgccg ggcagcttcg aaatcgccgg cggatttccg
     3661 cgcgctcgcg ctgtacgctc ccttcgccgg caacacgccg gccgactatc tcttcaagga
     3721 tgccgcctac gacaccgata cgctggcgct tatcgccggc agcgagcccg acctcatccc
     3781 gctcggcggg atcgatctcg tcgtgaacct gatctcggat gcggaccagg ccgaagcggt
     3841 gctgccggcg gcggcacgcc tggtcgcaaa gctcggcaag cccgtggtga atgatcccgg
     3901 caagatcagc cgcacgacgc gcgatgcagt tgccgcgctg ttgccgggga tcccgggctg
     3961 ccgcattccg aaaatcctgc gtcttgatgc cggtgcagac gtctcggtgg ccgcgctcga
     4021 gagcctgttg ccgtttccct ttcccgtgct ggcccggccg gcagggaccc acggcggcga
     4081 cgatttcgag aagatcgagg acctcgatgc attgtcgctg tttctcaaat gtcgtccggg
     4141 cgacgaccac tacgtgatcg aatatgtcgc gtacgcctcg ccggacgggc actttcgcaa
     4201 ataccgcttc atcttcgtag gccacgaaat cctgccctat catctcgcga tcggtaacga
     4261 ctggaaggtc catcacgcct cgaccgatat ggcgaaccag ccctggatgc agcgcgagga
     4321 agcggccttc ctcgccgatc ctgccgcggt gttcaagccg gcgaattacg cagcgttgcg
     4381 tgccgtgtgc gaacgcatcg ggctcgatta cttcggcatc gattgcgcgc tggatgatcg
     4441 cggagacgtc ctggtgttcg aggtcaatgc ctcgatgctg gtgcacgacg acaatgccga
     4501 attttcctac aaggacccgg ctgttcgcgc catcaaatcg gctttcgatg ccatgttgca
     4561 agcacgcgcc ggcgcgtgac cccaacgcgt gccctgtgtt aaacttgcct tactagcttt
     4621 ggagcgtggg atggggcggg caagactggg attgcagaca tttttcgccg cgatcatcgc
     4681 gctctgcgtt gtcgcggtcg aaagcccggc gcttgcgaga atgctgctgg aaaatcgtga
     4741 tcccgcgcag ggcaataccg aggcagacgc gaccatcgcc ggcgtctggg tcgggaccta
     4801 ttcctaccgg aacgaccgtg catccgtagg cttcaccttc ttttttcctc ccgcgaccga
     4861 caatagctgc atcgggcgca gcgaagagca gaacacgttc ggcaacagga gcgcgccgaa
     4921 actttacgcg atcctgtcat gctcggagac ggcgctgtat ccgggccaga ccctgacgat
     4981 caggaagacc tatgacggca ccgggggcgt gtcgcacacc gtcatgtaca ccgggaaagt
     5041 atcggcggac cttcgcacca tttcagggga atgggtgctc aacgatatca ggggcacctt
     5101 cactatgtca cgctgaagca ggctccggcg atcacggcag atagagatat tgcagcgtgg
     5161 tccgcccgac ggcttcgaga ctcccgaccg aacatttgtc ggcggtgtcc tgcgtcgtgt
     5221 tgaagaacgg ctcgtactcg aaatcgatca ccagaaagct cgggatgccg gctgcggcga
     5281 gcgcggtatg gtcgtcgctg atcggatagg agatcatgtg cgtatcgaag gccgacggcg
     5341 ctaccgtctg cccggtgcgc cagtatttct gtacttccgg caacgcgctc gcaaccgatg
     5401 acggttcggg cctgagcttg agatccctgt cgcagaccat gtcgaacacc acggctttct
     5461 ccggcttggc gttcgggtag aaatccctga gatgcgcggc aaagtagggc gagccgagcg
     5521 ctttccagtt gggatcgccg gcgccgagcg agcgcgggcc ttcctccccg tcgaagaaga
     5581 tcatgtcgat tccgaccgcg ggctttggca gcagcgacag cacgcgggca gtttccagca
     5641 acacggcgac accggaagcc gaattgttgg cgccgggcat cggcccttgc ggattgctct
     5701 tgtcggcata ggcgcggacg atgctgtcat aatgcgttgc gacgatgatg cggcgcggat
     5761 tggatgggtc cagtcgcgcg atgatgttgg tcatcgcata ggatttgccg tcgtcgctcc
     5821 tgtatgccca gcgctgggtc gaaacatcgt cgattccggc tttggcgagc tcggacttga
     5881 tgaaatcgat ggtctgctga tgtccgggcg tgtcgagcga gcgtggcgta aagcgcagtt
     5941 gctcgttgat cagcgtcatg gcgcggttgc cgtcccacag ctcgatcttg acgtggtcgg
     6001 ccttgccttg ctcgccaacc tctgcacggc tggcagccga aggcagtgca agcgcgagcg
     6061 ctgtcgcagc cagcgcggaa agcttcgctc gtcggaaggt atccattctc ggtatcgtcg
     6121 ccttcatctt ctgccgaacg gcagaaagag cacgccggcg cagcaggcga gcgtcagcgc
     6181 aaagatgtag gtgatcgcac gtgagccgat ggtcgggatc agcgtaaagg tcgtgagcag
     6241 ggtgccgacg acgttgccga tcgtggagac gccgtagacg aggccggcca cgcggccggc
     6301 ttcctccgcc gagcgcgtca gaatgctgac tgcgatcgga gagaacgtgc cgagcagcga
     6361 cagcggcacg agcagcagcg cggtcgaggc ggtgagcgtc gccgccggtc cgtcgccgag
     6421 cccgctcagg attttttcca tcacgccgtc ggcggttgcg ggcacgcagg cgagataggc
     6481 ggcggcgatc aggatgaaga ccccgatggt ttgttgcgtg gggtgatggg cgatcattgc
     6541 gctgcccgcg aaatagccga tcgcgagcgc gcacaagacg gtcgagatca ggctcgccca
     6601 tgtaccgata ccgccgccga aataggggaa aagatagcgg ctgcccagca tctcgaagcc
     6661 catcagcacg ccgccgacaa tgaaggcgat gacgtagatc gtgaacgcca gaacgcggaa
     6721 gcgactcgtc aaggcaattt cctgttgtgc cgctcgatgt tcttgagcgt ctcgaccggc
     6781 gcgaaatcgt cggtcagcac cttggcgttg ggatcgatca cgttgccgcc tgcgatctgg
     6841 atgtgccgcc gctgcgacag catctcgagc aggctgtagc gcaggccgta ggccttgtcg
     6901 cggtcgcggg cgacgcccgc aaggtcttca ggttttcgct ccgcgccgtc ataggcgacc
     6961 gtgacgatgt tgccgtccgc ccggtagaag tcgagttgcg ggaacaccgc gttgatcgtc
     7021 ttgaccgccg aatcgaacag catggtcgaa ggctcgacgt tctgcgcgac cacgccgccg
     7081 tctgcgaggt gttccctgac gatttgataa aactccttgg tcagcagatg gaagggcacg
     7141 aacggaccgc gataggcgtc gatcaggatg aggtcgtaat gctccttcga ctcctgaaga
     7201 aacagccggc cgtcgcggtt caccaactgg aagttgggct cttccttgat cccgaaatat
     7261 ttcctggcga gttcgacaac ggtggggtcg agttcgaccg aggtcacctg gacgttcggc
     7321 agatagcggt gcaggtacca cgacgtgcga ccgccgccga agccgatctc gaggaccgag
     7381 tggacgtcct tcgcatacat caggctcgcg gtcatgaagc gcgtgtactc gaccggcagg
     7441 tcgcggtcgt cgcgggtatt gtagacgctc tcggtgtaga tccgccggtt gtggccgaag
     7501 gtcatgctga catagggcgg ctgttcgtac acgtaaatgt tattgtagag cgactccttc
     7561 gactccagca gattgcggct gtcctgggcg ggggctgcgg caaccccgac cagggcgccc
     7621 gctattgcgc ccaacatgac cgaccgaatg accatcgact gtctgcttcc cttcgtgctc
     7681 gaataccggt ttttgcgggc gccttattag actatcgctg cgcactcgga tagagaaaca
     7741 actcatccaa acgggggcgt gatgtccgcc ggaaggcccg taatctggct tgctgcaatg
     7801 atgatggctg gaactggatc ggttgcggcg gaagcgcctg catcgctggc cccgctgatg
     7861 gagcagaaac aggtcgtcgc ctccgagctg gcgcgccagg tggcgttttg cagccagcgt
     7921 aatgacaccg atcatcccgc cttcaggggt tgcgtcgatt ggcactccgc ggttcacggc
     7981 gtctgggcgc tgattgccta tcaacgggca accggggacg cgcaatacgc ggcgctggtg
     8041 gactcgatcc tcgagaagga ggcgctcgcg caagagcagg agcacttgcg gcaggcaccg
     8101 ctgttcgaga tgccctatgg gcgcgcctgg ttcctgcggc ttgcgatcga tcatcgcaaa
     8161 ttgaccggct cggacgatct cacggcgttc ggcaacgagg tggccttgag ccttcgcgat
     8221 tactatcgcc atcgcgccat cgatcgtttc tccgccgcct atcagagcgc ctcctgggcc
     8281 ctgatcaacc tcctcgacta tgcaagggat cgcaacctct ccgatctcga ggcggaggtc
     8341 acgggctgga tcaagacggg cttcgtcgat gtcgcgcccg catgcgcgaa cgagcgcgag
     8401 cgcggcgagt tcatggggat ctgcaccaac tgggcggcgc tggtcgcacg cgtgctcgac
     8461 agggatcggt attcgaattg gcttgagacg ttcatcgccg cgaacggcct gcctgcgccg
     8521 ctggcccgtc agtccggcga tcatgaattc ggattgaatt tttcccgcgc ctggggactg
     8581 tgggatatgt acgcggcaag cggccgcgcc gacgtgctcg aagcctatgt cgcgcatttc
     8641 aacaacggct ttacgccggc gtcgaactgg agcggcagct acatggcagt cggtcactgg
     8701 gtcgcgcaat tcggcctgtt cgccctgcaa ccgctattcg ggccagaggc ggggcggtaa
     8761 agcgcaatcg actcgtcagc tcgcgcgcat gcgagggaga gtggccggtg cgctgaggtg
     8821 ccagaaatgc agccgtgggg tccaggatag cacggttgcc gccggcggtc atggtcgagg
     8881 caagggcgct gcgatctgct attgtctcca tgattgcaaa acgagatcga agcaagccat
     8941 gtaccagccg cccgccttcc gagaggatcg catcgaggtt cagcacgcgc tgatccgcgc
     9001 gcacccgctc ggcctcctga tcaccgcagg cccggccggg ctgctcgcca atttctttcc
     9061 attcttggtc gatgccgagg gcgacaaggg gcggctgcgc ctgcacatcg cgcgcgccaa
     9121 cccgcaatgg agggaactgg atgcgatcga ggaatgcctg gtcgtattcc aggggccgca
     9181 ggattatgtg acgccgtcct ggtatgcgac caagcgcgag accggcaagg tggtgccgac
     9241 ctggaactac gccacggtgc acgtctgggg acggccgcgc gtcgtcaacg acgatgcctg
     9301 gctgcgccgg cagatcgaag acctgaccgc gagccgtgag agcttgcgtg aggcgccgtg
     9361 ggcggtcggc gatgcgccgg cggagttcgt cgcgatgcag atgcgggcca tcgtcggcgt
     9421 cgagattccg atttcgcgca tcgagggcaa gtggaagatg agccagaacc gccccgaggc
     9481 cgatcggcgg ggcgtgatcg aaggctttcg cgcaatgggt gaagcgggcg aggagatcgc
     9541 cgccctggtt gccgaacgcg gagagcgctt gaaataaggc tgcggtcggt cgtctccaaa
     9601 gacaggattc gcacttccgc gatctgtcgc gatgctcgcc gcttgtttgc tgcatcgctg
     9661 ccaaattttc ccctcgaact gtctcaattc cgacgccgac tcggccgacc ctctgcttcg
     9721 ttgattcgta aattgaccgg actcggcggg gttcagttgc caggagtttc tgtcatggga
     9781 aatgtgtatc tgcccgcaga gaaggggaat ccagttcccg catcgcgaat tcaggacgag
     9841 gacgacggtt tcgactcgga catgaattcc gacatgatca agttgctgga agaaaacgcc
     9901 cggttgcggg cactggtgac ccagctctcg agcctcgtgc tcaagcacgt cgtcgaccag
     9961 aaataggtgt ttttcaaaac cgggctgcga gcgtcggacc agaaccggcg cgggcctgct
    10021 tttgcccgaa gccagcggct ttcgcgcggg gtttccgtgg cggacaaagg cttccgattg
    10081 ggcggcggct ctgctatgga acgcctcgaa aatccgcttc tcgcttggcg ggtttttgcg
    10141 gtcccgtagc tcagctggat agagcggcgg tttcctaaac cgtaggtcgc atgttcgagt
    10201 catgccggga tcgccattcg ccttgaaatc cgccgcgggt gtaacctccc gtctctcgcg
    10261 ttgaagattc cgcgcggccg cgcgatgttt gggcgatgaa ggacattgtt gcgcagaagc
    10321 tcaagatcga cgtcggcaca tcgggcacgg tttccgcgct cctgatgcgc ccgcgcgagg
    10381 cgcgcgcctg cttcgtgttc gcgcacggcg cgggcgccgg cataacccat cccgcgatcg
    10441 agaccatggc caccggcctt gccgagcgcg gcgttgcgac cctgcgctat cagtttccct
    10501 acatggagag gggcagcaag cggcccgatc cgccgggggt ggcgcatgcc gccgtacggg
    10561 ctgcagtcgc cgaggccgcg cgccgctgcg ccgggctcgc gctgattgcc ggcggcaaat
    10621 cctttggcgg gcgcatgacg tcgcaggccc aggcgaaggc gccgctcgac ggcgtcgagg
    10681 gccttgcctt cctcggcttt cccctgcatc cggccggaca gccctcgacc gatcgcgccg
    10741 cccatctcgc cgacatccac atcccgatgc tgttcctgca gggcgccaat gaccagctcg
    10801 caaaggtcga tctgctcaag cccgtcgtcg ccgggcttgg ccggcgcgcg acgctgcatc
    10861 tcgtcgaggg tgccgatcac tctttccacg tgccgaagcg atccgggcgc gacgacaagg
    10921 aggtgatggc cgaagtgttg gatgcgttcg cggcctggac cggcgtcacc gtggccaaac
    10981 gcggctgatc ccgccatccg tatcccaaaa ggcttcaaat cgccgtcgct ggcgtagccc
    11041 tgcggatcgt gcaatggtcc gcgcgccccg atgagaccgg agaaattgga atgctggacg
    11101 tgcagaatgc gatgaccgcg acgacgaaag agatggcgcc gccgcgatgg acgagcggca
    11161 cagccttctc tctttacatg caacccttca atgatctcct tttcagggcg cacgccattc
    11221 accgcgagca tttcgacccc aatcgcgtgc agctcagccg cctcctcaac atcaagaccg
    11281 gcgggtgccc cgaggattgc ggctattgca gccagtcgtc gcatcacgca acgggc
//
LOCUS       DATLJT010000342         8446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2087030,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000342 DATLJT010000000
VERSION     DATLJT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087030"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VM910_07920"
     CDS             <1..210
                     /locus_tag="VM910_07920"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158424.1"
                     /note="NADPH-dependent; catalyzes the reduction of
                     7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in
                     queuosine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HVI62500.1"
                     /translation="FRNHGAFHEDCTVMIGKRIFAEIKPKWLRIGGYWYPRGGIPIDV
                     FWQSGKLPKGLWVPDQGVAPYRGRG"
     gene            complement(220..1449)
                     /locus_tag="VM910_07925"
     CDS             complement(220..1449)
                     /locus_tag="VM910_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62501.1"
                     /translation="MLQTGLRPPLDQRLPPALNVIALATFSASLSVRALDPVLPHVAD
                     EFGVTIATAAGFASGFAFTFAIVQPLVGAAADLFGKARLMIACLVLLGFANILGALSS
                     SFPLLFATRILAGIGSGGIFPVALGLTSDLVGSEKRQIAIGRTLAGSMTGNLLGASAS
                     GLIGDFLGWRGVLAVLGALVVVAAIAVGAGFRSAALSPPPGMSFSALKAGYRTIFTNP
                     NARICYSAVFVEGCCVLGLFPFIAAFLFELGETSLSVAGIVIAGFAIGGLFYTFTVSR
                     FLPRLGVRGMMTVGASLVALQLAAIAFGPGWKIQFLILLLMGWGFYMIHGCLQVFASE
                     LSVEARATALSLHSFFFFMGQTIGPIAYGFGLAHVGKVPTLLIAASAMVALGFACARL
                     LRQRLPADAPANPDVEA"
     gene            complement(1453..2301)
                     /gene="kdsA"
                     /locus_tag="VM910_07930"
     CDS             complement(1453..2301)
                     /gene="kdsA"
                     /locus_tag="VM910_07930"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HVI62502.1"
                     /translation="MNATTFAAPIVNVGPVKFGNNLPISIIAGPCQLESRAHALEVAG
                     ALKEIAVRLKIGLVYKTSFDKANRTSAAGARGIGLKQSLPIFAEIRETLRLPVLTDVH
                     EAAQCADVAQAVDVLQIPAFLCRQTDLLLAAAATGMVVNVKKGQFLAPWDMANVVSKI
                     TGSGNRNVLVTERGASFGYNTLVSDMRALPILARTTGAPVIFDATHSVQQPGGKGTSS
                     GGEREFVSVLARAAVAVGVAGMFIETHPDPDHAPSDGPNMVPLSEFESLVRTLMAFDA
                     VAKSKT"
     gene            complement(2332..2658)
                     /locus_tag="VM910_07935"
     CDS             complement(2332..2658)
                     /locus_tag="VM910_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVI62503.1"
                     /translation="MIYEMRVYRCLPGRLPALLKRFDTITLKLWEKHGIKQAGFFTTL
                     VGESNQELTYFLAWDSLAEREKKWTAFMADPAWIEARAKTEADGQIVGNIVNQFLAPT
                     SFSSVK"
     gene            complement(2753..4384)
                     /locus_tag="VM910_07940"
     CDS             complement(2753..4384)
                     /locus_tag="VM910_07940"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVI62504.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYL
                     NLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPATRADNITTGRIYQDILTKE
                     RRGDYLGATIQVVPHVTNAIKEFILSGNDDYDFVLVEIGGTVGDIEGLPFFEAIRQLK
                     NELPRDHAVYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKE
                     ERRKLGLFCNVRESAVIEARDVDNIYAVPEAYHAAGLDDEVLAAFGIAPKIPPALQSW
                     HTINERIRNPEGDVTIAIVGKYTGMKDAYKSLIEALSHGGIANKVKVNLDWIESEVFE
                     NEDPAPFLEHVNGILVPGGFGQRGAEGKIRAAQFARERDVPYFGICFGMQMAVIEAAR
                     NLVGIEQANSTEFGPTPEPLVGLMTEWLRGNELEKRSRNGDLGGTMRLGAYPAILKRG
                     SRVSQVYGGATEISERHRHRYEVNTSYKDRLEQHGLKFSGQSPDGVLPEIVEYEDHPW
                     FIGVQFHPELKSRPFEPHPLFASFIQAAVVQSRLV"
     gene            complement(4581..4976)
                     /gene="secG"
                     /locus_tag="VM910_07945"
     CDS             complement(4581..4976)
                     /gene="secG"
                     /locus_tag="VM910_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HVI62505.1"
                     /translation="MQTVIIVVHLMIVVVLIGAVLLQKSEGGGLGMGGGAGFMSSRGT
                     ANLLTRTTAVLACGFFATSLLLSWLASLDRKPASIIGTQAPPAQSQPVSDAPTPVTPP
                     TSGGLLDTLKKADEQQNQAPSAPQAPKSQ"
     gene            5178..5744
                     /locus_tag="VM910_07950"
     CDS             5178..5744
                     /locus_tag="VM910_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVI62506.1"
                     /translation="MDTIIDDIGQRLAQRIRLERDSRGWSLADLAQRSGVSKATISKI
                     ERDEVSPTAAVLVRLASAFGLTLAGLMLRAEARGERLSRADEQPVWRDPETGYVRKQV
                     FSRPDHPVEIVRVELPPRQRVTLPASSYAHIRQLVWVQAGTLMIAGGGERHVLGAGDC
                     LGFGPPADTTFANETGSTCVYVVALARS"
     gene            5747..6277
                     /locus_tag="VM910_07955"
     CDS             5747..6277
                     /locus_tag="VM910_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI62507.1"
                     /translation="MAEITIAPLTDLPRIQRGLSVLLVETVASGGSVSFMHPLPLESA
                     RAFWRDSLASAARGERIVLGAFDGDDLVATVTLLLDLPPNQPHRAEIAKMMTKLSYRH
                     RGIATALMRAAEKMALERGRTLLVLDTAVEDGASRLYEGLGFNLTGVIPDYSLKPHGG
                     LTGTMIYWKRIAAETA"
     gene            complement(6274..7029)
                     /gene="tpiA"
                     /locus_tag="VM910_07960"
     CDS             complement(6274..7029)
                     /gene="tpiA"
                     /locus_tag="VM910_07960"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVI62508.1"
                     /translation="MTNPIRPLIAGNWKMNGLKASASEFDAMLAGASAVSGKADLLVC
                     PPATLVAAFAERAKASKAIHIGAQDCHPKPSGAHTGDISAEMLADAGAVAIIVGHSER
                     RADHGESDALVRAKAEAVWRAGLTAIVCIGETREQRDAGKTLQVCGGQLAGSVPEAST
                     AGNLVVAYEPVWAIGTGLTPTPADVEQVHRFIRESLTQRFKGEGQMIRILYGGSVKPS
                     NAAELLGVTNVNGALVGGASLKAADFLAIAAGC"
     gene            7273..>8446
                     /locus_tag="VM910_07965"
     CDS             7273..>8446
                     /locus_tag="VM910_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVI62509.1"
                     /translation="MLRGLRKASSNWLGKTVMTVAMGALIVSFGIWGIADIFRGFGQS
                     TLAKVGGTEISLNEFRQIYSDRLQQIGRQFGRPLTQDQARAFGFDRQVLQQTIAEAAL
                     DEKARKLGLGQSDAETMRLIVADPSFKGLNGTFDPQRFQGMIRQFGFTEGRYLAEQKR
                     VSLRRQLAGTITAGLEPSKTLLEALSHFQNEQRSIEYVRLGAAQAGQIDPPSPETLAG
                     YFDEHKGQFRAPEYRTIAFVSITPEEIGKWSEVSDEDAKKLYEQRKDRFGTPEKREVS
                     QIVFPNMEEAQAARNGIASGTSFDDIAKERKLNPADVDLGLVTKSAILDPAIADAAFS
                     LPSGEVSQPVQGGFGVALVKVGTIQPAVERDYASVATDLKREIAAERARTQVAALRD"
BASE COUNT         1654 a         2627 c         2636 g         1529 t
ORIGIN      
        1 ttccgcaatc atggcgcatt tcatgaagac tgcacggtca tgatcggcaa aaggattttt
       61 gcggagatca agccgaaatg gctcaggatc ggcggctatt ggtatccgcg cggcggcatt
      121 ccgatcgacg tgttctggca gagcggcaag ctgccgaagg gcttgtgggt tcccgatcag
      181 ggcgtcgcgc cctatcgcgg acggggttag gcaaaaacat cacgcttcca catccggatt
      241 tgccggggca tccgccggca gccgctgccg caacagccgc gcgcaggcaa accccaaggc
      301 gaccatcgcg ctcgcggcga tcagcaaggt cggcaccttg ccgacatgcg caaggccaaa
      361 gccgtaagcg atcggcccga tggtctggcc catgaagaag aagaacgaat gcaacgacag
      421 cgcagtcgcg cgtgcttcca cggaaagttc actggcaaag acctgcaggc agccgtggat
      481 catgtaaaag ccccagccca tcagaaggag gatcaagaac tggatcttcc accccggacc
      541 gaacgcaatg gcggcgagtt gcaaggccac cagacttgcg ccgacagtca tcattcccct
      601 gacgccgagc ctgggcagaa accgcgagac ggtgaacgta taaaacaaac cgccgatcgc
      661 gaacccggcg atcacgatgc ccgcaaccga tagcgatgtt tcgccgagtt cgaacaggaa
      721 ggcggcgata aacggaaaca gtccaagcac gcagcagcct tcgacgaaca cggccgagta
      781 gcaaatgcgc gcgttgggat tggtgaagat cgtgcggtag ccggccttga gagcagagaa
      841 actcatcccg ggtggcgggc tgagcgcagc gctgcggaag cccgcaccaa cggcgatcgc
      901 ggcaacgacg acaagcgcgc cgagcacggc cagcacgccg cgccagccga ggaaatcgcc
      961 gatcaggccg gaggccgacg cgccaagcag gttgcccgtc atcgagcccg ccagcgttcg
     1021 tccgatcgcg atctgacgct tctcggagcc gacgagatcg ctggtgaggc ccagcgcgac
     1081 cggaaagatg ccgccggaac caataccggc gaggatgcgc gtcgcgaaca gcaggggaaa
     1141 ggaggacgac agcgcgccga ggatattggc aaaacccagc aacaccaggc aggcgatcat
     1201 cagccgcgcc ttgccgaaca ggtcggctgc cgccccgacc agcggctgca cgattgcgaa
     1261 ggtgaaggcg aagccggaag caaatcctgc ggcggttgca atagtcacgc cgaactcgtc
     1321 ggcgacatgc ggcagcaccg gatcgagtgc gcgcaccgac aggctggccg agaaggttgc
     1381 aagcgcaatg acgttgagcg ccggcggaag gcgttgatcg agcggcggcc tcaggccggt
     1441 ttgaagcatc gctcaggtct tgcttttggc gaccgcgtcg aatgccatca atgtccgcac
     1501 caaactctcg aactcgctga gcggcaccat gttcggacca tcggacggcg cgtgatcagg
     1561 atcgggatgg gtttcgatga acatgccggc cacgccgacc gcgaccgcgg cgcgcgccag
     1621 cacggaaaca aactcgcgtt cgccgcccga tgatgtgccc ttgccgccgg gctgttgcac
     1681 cgaatgggtg gcatcgaaaa tcacgggcgc gccggtggtg cgcgccaata tcggcagcgc
     1741 gcgcatgtct gaaaccagcg tgttgtagcc gaacgatgcg ccgcgctcag tcaccagcac
     1801 gttccgattg ccgctgccgg tgatcttgga gaccacattc gccatgtccc agggcgccag
     1861 aaactgcccc ttcttgacgt tcacgaccat ccccgttgca gccgccgcga gcaagagatc
     1921 ggtctgccgg cacaggaacg ccggaatctg caacacgtcc acggcctgcg ccacatcggc
     1981 gcattgtgcg gcctcatgga catcggtcag cacgggcaat ctcagggtct cgcgaatttc
     2041 cgcaaaaatc ggcagagatt gcttcaggcc gatgccgcgc gctcccgcgg cactggtgcg
     2101 attggccttg tcgaaggagg tcttgtagac gagcccgatt ttcaaacgga cggcgatctc
     2161 cttcagcgca cccgccactt ccagcgcgtg cgcccggctt tcgagctggc acggcccggc
     2221 aatgatcgaa atcggcaggt tgttgccgaa tttgaccggg ccaacgttaa cgatcggcgc
     2281 agcaaaggtc gtggcgttca agaaaccatc ctttccggcg cgacgccgtg ctcacttcac
     2341 cgaagaaaat gaggtaggcg cgaggaactg gttgacgatg ttgccgacga tttgtccatc
     2401 cgcctcggtt ttcgcgcgcg cctcgatcca tgccggatcg gccatgaagg ccgtccactt
     2461 cttctcgcgc tccgccagcg agtcccaggc cagaaaatag gtgagctcct ggttggattc
     2521 gccgacgagc gtggtgaaga aaccggcctg cttgatgccg tgcttttccc acagcttcaa
     2581 cgtgatggtg tcaaagcgct tcagaagcgc cggcagccgg cccggcaggc aacgatagac
     2641 gcgcatttcg tagatcatcg agacactccc ttgtgctttt gttcttgcgg tcgcgggggc
     2701 gtgttgtcgc aacaaaccgg gcgttcgtca aaggcaggct gaccgtattc gcctacacca
     2761 gccggctctg caccaccgct gcctgaatga aggacgcaaa caatggatgc ggctcaaacg
     2821 gccgcgactt caattcggga tggaattgca ccccgataaa ccaggggtgg tcctcgtatt
     2881 ccacgatttc aggcagcacc ccgtcgggcg actggccgga aaatttcagc ccgtgctgct
     2941 ccagacggtc cttgtagctg gtgttgacct catagcggtg acggtggcgt tcggaaattt
     3001 cggtcgcacc gccatagacc tgcgacaccc ggctgccgcg cttgaggatc gccggatagg
     3061 cgccgagccg catcgtgccg ccgagatcgc cattccgcga acgtttctcc agctcattgc
     3121 cgcgcagcca ttccgtcatc aggccgacta gcggttccgg tgtcgggccg aattcggtgg
     3181 agttggcctg ctcgatgccg acgagattgc gcgcggcctc gatcacggcc atttgcattc
     3241 cgaagcagat gccgaaatag ggcacgtcgc gctcgcgcgc gaactgcgcg gccctgatct
     3301 ttccttccgc accgcgctgg ccgaagccgc ctggcaccag gatgccgttg acgtgctcga
     3361 ggaacggcgc gggatcctcg ttctcgaaca cctcgctctc gatccagtcg agattaacct
     3421 tcaccttgtt ggcgatgccg ccatgggaga gcgcctcgat cagcgactta taggcatcct
     3481 tcatgccggt atatttgccg acaatagcga tggtcacgtc gccttcggga ttgcggatgc
     3541 gctcgttgat ggtgtgccag ctctgcagcg ccggcggaat tttcggcgcg atcccgaacg
     3601 ccgcgagcac ttcgtcgtcg aggccggcgg cgtgataggc ctccggcacg gcgtaaatgt
     3661 tgtcgacgtc gcgcgcctcg atcactgcgc tttcgcgcac attacagaac agcccgagct
     3721 tgcgccgttc ttccttcggg atcggccggt cggtccggca cagcaggatg tccggctgga
     3781 tgccgatcga gcgcagttcc ttcacggaat gctgggtcgg tttggtcttc aattcgccgg
     3841 cgctcgggat gtagggcaga agcgtcagat ggatgtagac cgcgtggtcg cgcggcaatt
     3901 cgttcttgag ctggcggatc gcctcgaaga acggcaggcc ctcgatatct ccgaccgtgc
     3961 cgccgatctc gaccagcacg aaatcgtagt catcattgcc ggaaaggatg aactccttga
     4021 tcgcgttggt gacgtgcgga acgacctgga tggtcgcgcc gagataatcg ccgcggcgtt
     4081 ccttggtgag gatgtcctga tagatgcgcc cggtcgtgat gttgtcggcc ctggtagcgg
     4141 ggcgtccggt gaagcgttcg taatggccga gatcgaggtc ggtctcagcg ccgtcatcgg
     4201 tcacgaacac ttcgccgtgc tggtacggcg acatcgttcc gggatcgaga ttgagatagg
     4261 gatcgagctt gcgaagccgg accttgtagc ctcttgcctg caggagggcc ccgagtgccg
     4321 ccgaagccag acccttcccg agcgaggaaa ccacgccgcc ggtgataaag atgtaccgcg
     4381 ccatgggact ttacctttaa ggccagcggg ccgattcgcc aaaacgattc gcgtaccccg
     4441 gcaaataatc tgcagggctg tgggtgaaga acgaaactga tgatctttca gttacttgat
     4501 ggggatttct gacgcagagc gctagccgcc ctgctgcatg gccaccctgc tttattgcgg
     4561 cttattgaga atgacgcggc ttattgggac ttcggcgcct gcggtgcgct cggggcctga
     4621 ttctgctgct cgtcagcctt tttcagcgtg tccagcagac cgccgctggt cggcggtgtc
     4681 accggcgttg gagcgtcgga gaccggctgc gattgcgcgg ggggcgcctg ggtgccgatg
     4741 atcgaagcgg gcttccgatc aaggctcgcg agccaggaca gaagcaggct cgtggcaaag
     4801 aagccgcacg ccagcactgc cgtcgtgcgg gtcaggagat tggcagtacc gcggctcgac
     4861 atgaagccgg ccccaccgcc cattccgaga ccgccgccct ctgatttttg cagcaacacc
     4921 gcgccgatca ggacgacgac gatcatgagg tggacgacga tgatgacagt ctgcatattg
     4981 accttccatc acgagccgag atcgccgatg cggcggatcg caactcgcgg gctttccagg
     5041 gaatgcgcgg ggtgtacacg atcggaccgc gcgttgtcac ccccgacatt aagggtgccg
     5101 attcttcccg caagcagcct tggccgcaga tttttcgggg cttgtgaaac gatcatatcg
     5161 tctactatag tagacatatg gatactataa tagacgacat cggtcagcgg ctggcacagc
     5221 gtatcaggct cgaacgcgac agccgtggct ggtcgcttgc cgatctcgca cagcgctccg
     5281 gcgtctccaa ggcgaccatc agcaagatcg agcgcgatga agtcagcccg actgctgcgg
     5341 tcctggtgcg cctggccagt gccttcggtc tcacgctcgc cgggctcatg ctacgcgctg
     5401 aggcacgagg tgaacgtctc tcccgcgcag acgagcagcc ggtatggcgc gatcccgaga
     5461 ccggatacgt ccgaaagcag gtgttcagcc gtcccgatca tcctgtcgag atcgtgcggg
     5521 tcgagctgcc gccgcggcaa cgagtaacgc tgcccgcatc ctcttacgcc cacattcgcc
     5581 agctcgtctg ggttcaggcc ggcacgctca tgatcgccgg gggcggcgag cggcatgtcc
     5641 tcggcgcggg cgattgtctg ggatttggac cgccggcaga tacgaccttt gccaacgaga
     5701 cgggcagcac ctgcgtctat gtcgtggccc tcgcccggag ctagacatgg cagaaatcac
     5761 tatcgctcca ttgacagacc tgccgcggat ccaacgcggg ttgagcgtac ttttggtgga
     5821 aacggttgca agcggcggct cggtgagctt catgcatccc ctgccgctcg aatccgcccg
     5881 ggcattctgg cgtgactcgc tggcgtccgc ggcccgcggc gaacgcatcg tgctcggtgc
     5941 gttcgatggc gacgacctgg tcgcaacggt cacgttgctg ctggacctcc cgccgaacca
     6001 accgcatcgc gccgaaatcg cgaagatgat gacgaagctc agttaccgcc atcgcggcat
     6061 tgcgaccgcg ctgatgcgcg cggcagagaa gatggccctc gagcgcggac gcaccctgct
     6121 cgtgcttgac actgcagtcg aggatggcgc ctcacggctc tacgaaggat tgggtttcaa
     6181 cctgacaggc gtcattccgg attattcgct gaagcctcac ggcgggctca cgggcacaat
     6241 gatctactgg aagaggatcg ccgcagagac ggcctagcag ccggccgcga tcgccaggaa
     6301 atcggcggcc ttcaggcttg cgccacccac cagcgcgccg ttgacattgg tcactcccaa
     6361 cagttcggcg gcattcgatg gcttgaccga gccgccatag agaatgcgga tcatttgtcc
     6421 ttcgcccttg aatcgctgcg tcagggactc cctgataaac cgatggacct gctcgacatc
     6481 tgcaggcgtg ggcgtaagcc cggtgccgat cgcccagacc ggctcatagg cgaccaccag
     6541 gttaccagcg gtcgacgcct caggcacaga accggcgagc tgtccgccgc aaacctgaag
     6601 tgtcttgccg gcgtcgcgct gttcacgggt ttcgccgatg cagacgatgg cggtgagccc
     6661 cgcccgccac acggcttccg cctttgccct gaccaaagca tcgctttcgc catgatcggc
     6721 gcgccgttcg gagtgcccga caataatcgc aacggccccc gcatcggcca gcatctcggc
     6781 agagatgtcg cccgtatggg ctccggacgg cttgggatgg caatcctgcg ctccgatatg
     6841 gatcgccttt gacgccttcg ccctttccgc gaacgccgca accagcgtgg cgggcgggca
     6901 aaccaaaaga tcggccttgc cggaaacggc agacgcgccc gcgagcattg cgtcgaactc
     6961 cgaggccgag gccttgagcc cgttcatttt ccagttcccg gcgatcaacg gccgaattgg
     7021 gttggtcatg gtaaaaattc cggtccagat aaaattgcgg tcgggaagaa gatggagcag
     7081 cgctagcaga gcgcgccctt gagttccaga cggcgatttt ggccattaaa gcggggcttt
     7141 ggtccagact cgcctgcccg ctcaaggttg cgaccgggca gcggccactt tatgatgtca
     7201 attcggtgat gaccttttgc ggaatccgcg gtaaggcgcg ccctttctct ccctgaaaga
     7261 caagttggac ccatgcttcg aggattgcgc aaggcctcat caaactggct cggcaaaacg
     7321 gtcatgaccg tggccatggg cgcgctgatt gtcagtttcg gcatctgggg cattgccgac
     7381 attttccgcg gtttcggtca gtcgaccctc gccaaggtcg gcggcaccga gatttcgctc
     7441 aacgagttcc gccaaatcta tagcgatcgg ttgcaacaga tcggccggca attcgggcgt
     7501 ccgcttaccc aggaccaggc acgcgccttc ggcttcgatc gacaggtact gcagcaaacg
     7561 attgccgagg ccgctctcga cgaaaaggcc cggaagctgg gactggggca atccgacgcc
     7621 gaaaccatgc gcctgatcgt tgcggatccg agtttcaagg gtctcaacgg caccttcgat
     7681 ccgcagcgat tccagggcat gatccgccaa ttcggtttca cggaagggcg ctatctcgcc
     7741 gagcagaagc gggtttcgct gcggcgacag ctcgccggca cgatcaccgc gggactggag
     7801 ccctcgaaga cgctgttgga ggcgctgagc cacttccaga acgagcagcg ttcgatcgaa
     7861 tatgtccggc ttggtgccgc gcaggccggc cagatcgatc cgccctctcc ggaaacgttg
     7921 gccggctatt tcgatgaaca caagggtcag ttccgggcgc ccgaatatcg cacgatcgcc
     7981 ttcgtctcga ttacccccga agagatcggc aagtggtctg aggtttccga cgaggacgcg
     8041 aaaaaactct acgagcagcg caaggacagg ttcggcacgc cggagaagcg agaggtatcg
     8101 cagatcgtgt tcccgaacat ggaggaggcc caggccgcac gcaacggcat cgcgtcggga
     8161 acgtcgttcg acgacatcgc caaggagcgc aagctcaatc ccgccgatgt ggatctcggt
     8221 cttgtaacca aatccgccat tctcgatccc gcgatcgcag atgctgcgtt ttcgcttcct
     8281 tccggcgagg tgagtcaacc ggtgcaaggc ggtttcggcg tcgcgctcgt caaggtcggc
     8341 acgatccaac cggcggtcga gcgcgattac gcgagcgtgg ccaccgacct gaagcgcgaa
     8401 atcgcggccg agcgcgcccg aacccaggtt gccgcactgc gcgaca
//
LOCUS       DATLJT010000343         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3465551,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000343 DATLJT010000000
VERSION     DATLJT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465551"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VM910_07970"
     CDS             complement(<1..250)
                     /locus_tag="VM910_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62510.1"
                     /translation="MFARFVLGTITAGAMIMPALAGMMTAEEARKFVAGKVFAFTCFD
                     GTRGAGRILDDMGVSGAVQFSGSGPIRRVRLPGNTLQIR"
     gene            complement(355..1341)
                     /locus_tag="VM910_07975"
     CDS             complement(355..1341)
                     /locus_tag="VM910_07975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HVI62511.1"
                     /translation="MAMFKAIRIDKADKGTTAALTQFDEADLMEGDVTVRVEWSTLNY
                     KDGLAVTGKAPVVRRFPMIAGIDFAGTVEQSSHPKWKPGDRVICTGWGMGETHLGAYA
                     EKARVKGDWLVALPQGLSAREAMAVGTAGFTAMLAILALEKHGLSPQSGPVVVTGAAG
                     GVGSVATAVLSKLGYHVMASTGRMSEAGYLKGLGASEVIDRNELSAPAKPLGKERWAG
                     GVDSVGSNTLANLLSMTKYGGAIAACGLAAGMDLPSSVAPFILRGVCLLGIDSVMCPL
                     PAREAAWQRLARDLDRGKLSGITSEISLDQVIPIGGKILAGGVRGRVVVKIS"
     gene            complement(1389..2639)
                     /locus_tag="VM910_07980"
     CDS             complement(1389..2639)
                     /locus_tag="VM910_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62512.1"
                     /translation="MSTTAGDLRPASNAASWRTPLVIIVCGCAIGLLSFGPRSALGFF
                     IQPMNHDFAWGRDVFALALAVQNLLWGLGQPLAGAIADRFGTFRVMLVGALLYAGGLL
                     MMRYATSPLSLNLGAGVLVGFGLSGCSFNLVLSSFSKLLPPEKRGLALGAGTAAGSLG
                     QFLFAPIGVAMIDGLGWQGALTVFAALMLLIIPVSLALNTPPASSDHLPAAQQQSFKT
                     ALAEAFGHRSYVLLVLGFFTCGFQLAFITVHLPAFLVDRGISAQIGGWVIAAIGLFNI
                     IGSLSVGWLQNFFPKRYILSVIYFLRAVATVVFVVSPVTPFSAIAFGAVSGLLWLSTV
                     PPTSALVSLMFGTRWFATLYGFAFVSHQIGGFLGVWLGGLAFEHFGSYGPVWWLSVLF
                     GVLSALINLPIVEKPVARAVAQPA"
     gene            2942..>3466
                     /gene="nadA"
                     /locus_tag="VM910_07985"
     CDS             2942..>3466
                     /gene="nadA"
                     /locus_tag="VM910_07985"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVI62513.1"
                     /translation="MPIAAIYGPEDFEGPAHSPDKPGAQRTGRDSVGARALPMPPLEW
                     NDEVARTTAHLYERVKSVLPPIEWPLMAPTIKAINELKAVRGAVILAHNYQTPEIFHC
                     VADITGDSLQLAIEASKVKADTIIQCGVHFMAETSKILNPHKTVLIPDSRAGCSLASS
                     ITGADVRLLRERFPA"
BASE COUNT          744 a         1123 c         1050 g          549 t
ORIGIN      
        1 cgcggatctg cagcgtgtta cccggcaggc gaacgcgacg gatcgggccc gagcccgaga
       61 actgcactgc gcccgaaact cccatgtcgt cgaggatgcg gccggcgcct cgggtgccgt
      121 cgaaacaggt gaaagcgaat accttgccgg ccacgaattt gcgcgcttcc tcggcggtca
      181 tcatcccggc cagcgccggc atgatcatcg cgccggccgt gatggtcccc aacacaaaac
      241 gcgcaaacat gtttatcact ccaactcaga gcgggcgaga cgcctttacc cgctccttac
      301 catactaacc gtggcaaaga ttggagcacg ttggttggta aagtctgaac gccattagga
      361 aatttttacc acgacgcggc cgcggacccc tccggcgagg attttcccgc ctatcgggat
      421 gacttggtcc aggctgattt cgctagtgat tccagatagt ttaccccggt ccagatcgcg
      481 ggcgaggcgt tgccaggcag cttcccgtgc tggcaaggga cacatcacgg aatcgatgcc
      541 cagaaggcac actccgcgca aaataaatgg ggccaccgac gagggcaaat ccatgcccgc
      601 ggcaaggccg caggccgcga tcgcgccgcc atatttggtc atcgacaaga gattggcgag
      661 cgtgttcgag ccgacgctgt ccaccccgcc ggcccagcgc tccttgccga gtggcttggc
      721 gggagccgac agttcgttgc ggtcgatcac ctcggaagcc cccagcccct tcaggtagcc
      781 ggcttccgac atccgtccgg tcgaggccat cacgtgatag ccgagcttgg acagcacggc
      841 cgtcgcgacc gaaccgacgc cgccggctgc gccggtgacg acgacaggac cgctttgcgg
      901 ggagagcccg tgcttttcga gggcgaggat ggcgagcatt gctgtgaagc cggcggtgcc
      961 gaccgccatt gcttcacgcg ccgacagccc ctgcggcaac gcaaccagcc agtcgccctt
     1021 gacgcgcgct ttttccgcgt aagcgccgag atgggtttcg cccatgcccc agccggtgca
     1081 gatcaccctg tcgccgggct tccatttggg atgcgaggac tgctcgacgg tacctgcaaa
     1141 atcgatgccc gcgatcatcg ggaaacggcg caccacaggc gccttgccgg tgaccgcgag
     1201 gccgtccttg taattgagcg tcgaccactc gacgcgcacg gtgacatcgc cctccatcag
     1261 gtcggcttcg tcgaactgtg tgagcgcggc ggtcgtgcct ttgtccgcct tgtcgatccg
     1321 gatcgccttg aacattgcca cggcagaact cccctgaacg ttcatgttct caggggatgt
     1381 ttagccgatc aagccggctg cgcaaccgct ctcgcgacgg gcttctccac gatcggcagg
     1441 ttgatcagcg cggacagcac gccgaacagt accgaaagcc accagaccgg accgtaggaa
     1501 ccgaaatgct caaacgcgag cccgccgagc cacacgccga ggaacccgcc gatctgatgg
     1561 ctgacgaacg caaaaccgta cagggtggca aaccagcgcg tgccgaacat cagggacacc
     1621 agcgccgagg tcggcggcac ggtcgaaagc cacaacaatc cgctgaccgc accgaacgcg
     1681 atcgcggaga acggcgtcac cggagacacg acgaagacca cggtcgccac cgcgcgcagg
     1741 aaatagatga ccgacaggat gtaacgcttg ggaaagaagt tctgcagcca gccgacgctc
     1801 agcgaaccga taatgttgaa cagtccgatt gcagcaatca cccatccgcc gatctgcgcc
     1861 gagatgccgc gatcgacgag aaaggccggc aggtgcaccg tgatgaaggc gagctggaag
     1921 ccgcaggtga agaagccgag gaccagcagc acataggagc ggtgtccgaa ggcctccgcg
     1981 agtgccgtct tgaacgattg ctgttgcgcc gccggtagat ggtcggagct cgcgggcggc
     2041 gtgttcagcg caagcgacac cgggataatc agaagcatca gtgcagcgaa caccgtcaac
     2101 gcgccctgcc agcccagacc atcgatcatg gcgacgccga tcggcgcgaa caggaactga
     2161 cccaacgagc cggccgcagt ccccgcgccg agcgcaaggc cgcgcttttc cggcggcagc
     2221 agcttgctga atgacgatag cacgaggttg aacgaacagc ccgagaggcc aaagccgacc
     2281 aggacgcccg ccccgaggtt gagcgacaga gggctcgtgg catagcgcat catcagaagg
     2341 ccgccggcat agagcagcgc gcccaccaac atcacgcgaa atgtcccgaa gcgatccgcg
     2401 attgcaccgg caagcggctg cccgagcccc cacagcagat tttgcacggc aagcgcaagc
     2461 gcgaacacgt cgcggcccca ggcgaaatcg tggttcatcg gctggatgaa aaagccgagc
     2521 gcggaccgcg gaccgaagct caaaagcccg attgcgcaac cgcagacgat gatgacgagt
     2581 ggagtgcgcc agcttgcggc attggaagcc ggcctaagat cgcctgcagt agtcgacatg
     2641 cattgccctg gttgttgttc gcggctccga atgtgcggcc gcgttggctt cagggagtta
     2701 atgcatctgc atgaaaatcc aatctgtcag gcccgcaaaa aatcggctcg caacgcagca
     2761 atgcaaaacc agccggtcag cccgaccaat tcgtgatcgt gagtcctctg gcgtggcgag
     2821 gagaaccgta ttatatttgt tttgctcgca tagagtataa ctaagccggc cccctgtctc
     2881 ggccggattt ctcggaccgc taatatactc ataatgagta tattagccaa gggaggccac
     2941 catgccgatc gctgcaatct acggccccga ggatttcgaa ggccccgcgc acagccccga
     3001 caaacctggc gcgcagagga caggccgcga cagcgttggc gcccgcgcac tgcccatgcc
     3061 gccgctggaa tggaacgacg aggtcgcacg caccaccgcc catctctatg aacgcgtcaa
     3121 atccgtgctc ccgccgatcg aatggccgct gatggcgccg acgatcaagg cgatcaacga
     3181 gctgaaggcc gtccgcggcg cggtgatcct cgcgcacaac taccagacgc cggaaatttt
     3241 ccattgcgtc gccgacatca ccggcgactc gcttcaactc gcgatcgagg ccagcaaggt
     3301 gaaggccgac acgatcattc agtgcggcgt tcacttcatg gcggagacct cgaagatcct
     3361 caatccgcac aagacggtac tgatcccgga ttcgcgcgcc ggatgctcgc tggcttcgag
     3421 catcacgggc gccgatgtgc ggctgttgcg cgagcgtttt cccgcc
//
LOCUS       DATLJT010000344         3922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4155251,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000344 DATLJT010000000
VERSION     DATLJT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4155251"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1728..2720
                     /locus_tag="VM910_07990"
     CDS             1728..2720
                     /locus_tag="VM910_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62514.1"
                     /translation="MSGMRRREFITLLGGAMAAWPLAARAQQSAIPVVGHLNVASAIP
                     YSHLLAAFRDSLKRAGWAEGLNVAFEYRWADGQYERLPMLAAELVRRQVTVIVASGGN
                     APAQAAKAATDIIPIVFVSGGEPVRGGLVASMNRPGANVTGVSTMFSALVPKRLDLLK
                     QLVPTITVIGALINPKYPDADLQRHELDDAAATIGQQVRLKEASTESEIEAAFASFAN
                     SRVDALIAMNDPFFQSVHDQITALAARHALPTIYWDREFAAGGGLVSYGPSLTDAFRQ
                     AGIYVARILRGEKPRDLPVQQPTKFELVLNLKTAKALGLQVPATVLALADEVIE"
     gene            2717..3715
                     /locus_tag="VM910_07995"
     CDS             2717..3715
                     /locus_tag="VM910_07995"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62515.1"
                     /translation="MRRREFIKLVGGAAAAWPLSVNAQPSAKIPRIGLMVTGSLQSPE
                     ARVQLDAFRQGLRQLGYTEGRNIAIEYRGADGRIERFPNLAAELVRLEVDLIFAANTR
                     AALAARQATSTIPIVSAVMGDPVEDGLVASLAQPGGNVTGLTFLAPELTAKRMQLLKD
                     ALPNVSRVAALWHPGAYGERTMDDMLKATESAAQTLGVQLQLIEVRAAGELERAFSTM
                     MKERAEALFLFPTPMLFLARRRIIELAATNRLPSVSQAREFVELGGLIAYGANINDLF
                     RRSTVYVDKILKGAKPADLPVEQPTRFELVINLKTAKTLGIDLPLGLMIRADEMIE"
BASE COUNT          768 a         1215 c         1212 g          727 t
ORIGIN      
        1 catcggcaat cattcgctca accttctgca cgacacgcgc aaagactgag ctcaggacgg
       61 acgtcgcgta gacccaatcg aattgtgcac cgctgccttc cataaggaag ctattttata
      121 caacggtatc agtcttgttg atacgccttt gctcctgtga gctccatcgg agaagagttt
      181 gggatggatg agacaccccg agcggggtca cgtcgtgccg tcgcctgagt gcagcgaccg
      241 ataggccagg cttagagggc agaacccgga tatcgccata gcgcgtcgat aagggcgttc
      301 acgcccgtct tcaacaggct atgaacgcgc gtgaacgcgc ttatggcgac atccgggcta
      361 cgctcagtga cgtcaggacg gcaggatgac ggccttgccg gtcgtctgcg tgtcgaagag
      421 ccggtaggct tcctcggcct gctcgagccg ccagcggtgc gtgaagagcc gctccacgtc
      481 caccttccgc tcggcgatga actcggcgca ctcggcctag ccttgcttcg agaacgtcca
      541 cgaccccacc agcgtgacct gccggcgcag gagatcggcg ctgacgtcga gcgtgacctg
      601 accccgctcg ccgacgaaac aggcggtgcc ccacgcgcgt accgagcgca ccgccgcggc
      661 acgggcggcc ggcgccgagg aggcgtcgag aaagtcgcgg cgccaccagg cggacgggcg
      721 gcgtatcgat ctcggcgcgg ctgcggcggg gcatgggttg aaaccgatga ccgcaaagga
      781 tcgtcagcgc atcgctatgc acctttagcg catcctaggg tcgtaggagc ttacgttcgc
      841 aaagggtgac aggtaatagc tgtcgcggga tgggggatag ggccaatagg tagtacgcac
      901 gtggagcctt acgggacgca cgaggagcct tacgggatag ctcgagtatg gctgcggcgc
      961 gtagtagctg gaatagtacg gagagctata gccgtaatat gagccatagc cgtatccggc
     1021 cactccagcc gctgcggctg cataagccgc gcggcggtat gtcctgcggt agagccttcg
     1081 atggatgccc gcaatgcttg ttactggccg ggcattagcg ctgttaagcg agagagatac
     1141 ctcatcttgc ggccattgca gcgaaaaggg tgccacagtc agcaaggcgg caccgccgat
     1201 aattccaagc ataccgagct ttttcatgtc tccgactcca aaggcacatt ccaaggcaca
     1261 acggcacaaa tggacctttc aaaatgtgct tcctccgaat gatgcgccga atcccccgaa
     1321 ttggcgagca caccccgcag gttgttagcc agtagaacga tccgcaaatc aaaccggggc
     1381 aggaggtagc attttttcag cgccgacgcg cccggaaggg tggcgtggtc ttaaaaaaat
     1441 cgacggcccc cgccgcgcag atcgaatgtc cgctttcggg ggcaaagcgg atattgccta
     1501 gtgcggcgtg aggtctgctg ttgacccaaa gcggacaaac gccggatcga gatcccgcag
     1561 tgcagcagtc tcctgccgca tcgtatggcg tgctatcctc tggtcgggct ggcgcagtgc
     1621 tcaacccgat tcagaacatt tcaggtcttg gccaaggact tttcggcctc gcttccatca
     1681 ggttgagcgt gcggtagacc ggcaatgccg agacgggagg aaccccgatg tccggcatga
     1741 ggcgacgcga gttcatcacg ctgctcggcg gtgcgatggc tgcgtggccg ctcgcggcgc
     1801 gcgcgcagca gtccgcgatt ccggtggtcg gacatctcaa cgtcgcttcg gccataccct
     1861 attcgcacct gcttgcggca ttccgcgaca gcctcaagcg cgccggctgg gccgaaggcc
     1921 tcaacgtcgc gttcgaatat cgctgggccg atgggcagta cgagcggctg ccgatgctgg
     1981 cggcggagct cgtgcggcgg caggtgacgg tgatcgtcgc atccggcgga aacgcgccgg
     2041 cccaagcggc caaggcggca accgacatca tcccaatcgt cttcgtaagc ggcggcgaac
     2101 cggttagggg cggcttggtg gccagcatga atcgccccgg ggcaaatgtg accggcgtga
     2161 gcacgatgtt ctccgcgctg gtgcccaagc gcctcgacct actaaaacag cttgtgccca
     2221 ccatcaccgt gatcggcgcg ctcatcaatc cgaagtatcc ggacgccgac ctgcaacgcc
     2281 acgagctcga tgatgccgcc gcgacaatcg ggcagcaggt tcgcctgaag gaagccagta
     2341 ccgagtctga aatcgaggct gctttcgcta gtttcgcgaa ctcgcgcgtg gatgccctga
     2401 tcgcaatgaa tgatccgttt ttccagagcg tccatgacca gattactgcc ttggcggctc
     2461 gccatgcgct ccccacgatc tactgggacc gcgagttcgc tgccggcggg ggcttggtca
     2521 gctatggtcc aagcctgacg gatgcgtttc gccaagccgg catctacgtg gcccgcattc
     2581 tccggggcga gaagccgcgc gatcttcccg tgcagcagcc aaccaaattc gagctggtgc
     2641 tgaaccttaa gaccgccaag gcgctcggcc tccaagtccc ggcgaccgtg ctcgcgctcg
     2701 ctgatgaggt gatcgaatga ggcggcgcga gttcatcaag ctcgtcggag gcgccgcggc
     2761 agcgtggccg ctgtccgtga atgcccaacc atcggcgaaa atcccacgaa ttggtttgat
     2821 ggtaaccggt tcgctgcaat cccccgaggc gcgagtgcag ctcgacgcct tccgacaagg
     2881 gctgcgtcaa ctcggatata cggagggtcg aaacatcgcc atcgagtacc gaggcgcgga
     2941 cgggaggatc gagcggttcc caaatctcgc ggcggagttg gttcgtctcg aggttgacct
     3001 catcttcgcg gcgaatacgc gtgccgccct tgccgcccgg caagcaacct ccactattcc
     3061 gattgtttca gcggtgatgg gcgatcccgt cgaggacgga ctcgtggcgt cccttgcgca
     3121 gccgggcggg aacgtcaccg gcttgacctt cctcgctccg gaactgactg ccaagcgaat
     3181 gcagctactc aaggacgcgc ttcccaatgt gtcccgcgtc gccgcgctat ggcatccagg
     3241 cgcatacggc gaacgcacga tggacgacat gttgaaagca acagagtccg ccgcgcaaac
     3301 cttgggagtg cagcttcagc tgatcgaggt gcgggccgcg ggagagcttg aacgcgcatt
     3361 ctccacgatg atgaaagagc gcgccgaggc actgttcctg tttccaaccc cgatgctctt
     3421 cctcgcgcga cgacgcatca tcgagcttgc cgcaacgaac cgactaccct ccgtgtccca
     3481 ggcgcgcgag tttgtcgagc tgggcggtct catcgcctat ggcgcgaaca tcaacgactt
     3541 gtttcggcgc agcacggtgt atgtggataa gatcctcaag ggcgccaagc ccgccgacct
     3601 ccccgtcgag caacccaccc gattcgagtt ggtcatcaat ctcaaaaccg ccaagacgct
     3661 cgggatcgac ctgccgctgg gcctcatgat ccgcgccgac gaaatgatcg aatgaggata
     3721 ctgtttttgc tgcactgcgt atgtccgctt ttgggcacaa agcggacatt cggaggggcg
     3781 cgctgtttgt ccgcttctct aatcggccgt ctgggtcaag cactttcaga ctaaccacca
     3841 ctccagtgtc gatgtcgctc acgggctcgc gcttctcttc ggactcggca ccaaggccct
     3901 tccatcatgg ggattcgaag ac
//
LOCUS       DATLJT010000345         4885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3006134,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000345 DATLJT010000000
VERSION     DATLJT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3006134"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..967)
                     /locus_tag="VM910_08000"
     CDS             complement(194..967)
                     /locus_tag="VM910_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVI62516.1"
                     /translation="MSEETAPLEITEDAFLGGQLRLRQPKSGHRAGHDAILLAAATSA
                     RPGARVVEFGAGVGVAGLALARRVGQLNLMLVEIDPRLAALARENAARNAIAAEVIVL
                     DVTAAADAFSAAGLAPDSADVVLMNPPFNDGERHRASPDRERAAAHMTHDATLESWLL
                     AARRILKSGGVLTLIWRADALSEVLSALARGYGSLVLQPVHADGSKPAIRVLIRAVKG
                     GKAPTRILSGLMLNDESGVPNKQVAEILAGKGGLRLAGP"
     gene            complement(960..1187)
                     /locus_tag="VM910_08005"
     CDS             complement(960..1187)
                     /locus_tag="VM910_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HVI62517.1"
                     /translation="MRELVRTNDIVLVSAIGALLDGANIHHLVLDQNMSIIEGSLGIL
                     PRRILVHDDDNEAARQILADAGLSHELRPDE"
     gene            1290..2300
                     /locus_tag="VM910_08010"
     CDS             1290..2300
                     /locus_tag="VM910_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVI62518.1"
                     /translation="MAVIVPFESPSGASIEALVSLVAADMERVNTTILARTGSEVTMI
                     PEVANHLISSGGKRLRPMLTLAMAHLSSYSGEGHIKLAAAVEFMHTATLLHDDVVDES
                     ELRRGKLSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVM
                     QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPAIANRPKAEQTACRSVGMNLGIA
                     FQLVDDVLDYGGKAAKLGKNIGDDFREGKITLPVVLAFRRGNDTERAFWIRALERGDI
                     GDGDLEHAIGLMTKHRALEDTMNRAQHYGAMAVDALALFPASAMKSALEQVVAFCLAR
                     SH"
     gene            complement(2364..3242)
                     /locus_tag="VM910_08015"
     CDS             complement(2364..3242)
                     /locus_tag="VM910_08015"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HVI62519.1"
                     /translation="MLSEVGRAKVNLTLRVVGRRVDGYHDLESVVAFADCADRLTLVP
                     GPDLKLEITGPLAQACGEIADNLVLKASGLLAARVPNLKLGTFALEKVLPVAAGIGGG
                     SADAAAALRLLAQLNDLPLDDERLKEVALETGADVPVCLRSHACDMTGIGESLLPLNP
                     PELPCLLVNPCVAVATKDVFSALGLRSGELLVGATDVIESPDWPDRGASVADWVAELS
                     LVGNDLEAPAMRIQPAIGEVLAALRESAGALLARMSGSGATCFAIYGNGGEALAAADK
                     IRRDHPDWWVHAGVLS"
     gene            complement(3269..>4885)
                     /locus_tag="VM910_08020"
     CDS             complement(3269..>4885)
                     /locus_tag="VM910_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI62520.1"
                     /translation="VERDATSAAAFYRSALRTDPKNNELLDRAFISSVADGDIDEAVK
                     LGERILTVDKSNRVARLVVGVQDLKLKKYAAAQLNINQSIRGPITDLVATLLSAWASY
                     GAGDTKTAIANIDKLTGPEWYPIFKDLHAGMILEQAGREKDAGARFERAYKLDDSMLR
                     VNEAYARWLSRNKGEAQATAIYEAFDKKLARHPLVLEGLRDTKAGKKMPPLVDSAQAG
                     AAEALYGIGATLIRRGGEDLALVYLQLALHLAPNHPLALLSLADLYESVKKPQMAIKV
                     YERVPAGSPLKRNAQIQLATDLDSVDRGDEAIKILKGVIADDPKDLEAIMALGNIERG
                     RKKFADCAETYSKGIDVLPEGNDKSNSVYYYYRGICEERSKQWPKAEADMRKALELQP
                     DQPHVLNYLGYSWIDQGVNLDEGMKMIKRAVEQRPDDGYIVDSLGWAYFRIGNFDEAV
                     KNLERAIDLKPEDPTINDHLGDAYWRVGRTLEAKFQWSHARDLKPEPEDLPKIEAKIE
                     NGLPDDPSSSSAASADKKKEDGKEEGNKGG"
BASE COUNT          870 a         1591 c         1528 g          896 t
ORIGIN      
        1 acgccactgt cattgctccg attggaacgc gctttgcata ccgttcgttg caggctgcga
       61 cgatgcagcg gggacagaca tgaaaatcta tctgctgatg atgctgatcg gtatcctttt
      121 ggccgcggtt cacttcactt ctgcgcctga acggccctcg aattcgcttc agcaatagcg
      181 cgtctgtcct gcgtcagggg cccgccagcc gcaagccccc ttttccagcc aggatctcag
      241 cgacctgttt attaggcacg cctgactcat cattgagcat caggcctgaa aggattctgg
      301 tcggcgcctt gccgcctttg accgcacgaa tcagcacgcg aatggcgggc ttgctgccat
      361 ccgcatggac cggttggagg acgaggctgc catagccccg cgcgagtgcc gacaacacct
      421 cgctcagcgc gtcggcgcgc cagatcagcg tcagcacgcc gccggacttg aggatccgcc
      481 gggcagccag caaccagctc tccagcgtcg cgtcatgggt catatgtgcc gcggcgcgtt
      541 cccgatccgg cgaggcgcgg tgcctctcgc catcgttgaa aggcggattc atcaggacga
      601 catcggcgct gtcgggcgca agacctgcgg ccgaaaaggc atcggccgct gcggtcacgt
      661 cgagcacgat cacttcggcg gcgattgcat tacgggcggc attttcgcgc gcgagcgcgg
      721 cgaggcgcgg atcgatctcg accagcatga gattgagctg cccgacgcgc cgggcaagcg
      781 cgaggccggc cacgccgacg cccgccccaa actccacaac gcgcgcgcca ggtcgcgccg
      841 aagttgcggc cgcaagcagt atcgcgtcgt gaccggcgcg gtgacccgat ttcggctggc
      901 gcaagcgcaa ctgcccaccg agaaacgcgt cctcggtgat ctccagcggg gcggtttcct
      961 cactcatccg gccgcaattc gtgcgacagg ccggcatctg cgagaatttg tcgcgccgcc
     1021 tcattgtcgt catcgtggac gagaatccga cggggcagga taccgagcga cccctcgatg
     1081 atgctcatat tctggtccag caccaaatga tggatgttgg cgccgtcgag cagcgccccg
     1141 atcgccgaaa ccaggacgat atcgttggtt cgaaccaatt cccgcaactg aacacgctcc
     1201 tctgcggcgc gaggaggacg ttcagccctt gccgcccctc gccgcacttt ctattgtcat
     1261 atcccaacat agcaaatgcg gagaccggtg tggcggttat tgtacctttc gaaagcccgt
     1321 caggcgcgtc gatcgaggcg ctggtcagcc ttgtggcggc cgacatggaa cgggtcaaca
     1381 ccacgatcct cgcgcgcacc ggttccgaag tcaccatgat ccccgaggtc gccaatcacc
     1441 tgatttcctc gggcggaaaa aggctgcgtc cgatgctgac gctggcgatg gcgcacctgt
     1501 ccagctattc cggcgaaggt cacatcaaac tcgccgcggc ggtcgagttc atgcataccg
     1561 caaccctgct gcatgacgac gtggtcgacg agagcgagct caggcgcggc aaattgtcgg
     1621 cacgtatgtt gtggggcaac gaggcgagcg tcctggtcgg cgatttcctg ctcggacaag
     1681 cgttccggat gatggtcgaa gtcggatcgc tgcgtgcgct cgacatcctc tccgctgccg
     1741 cggcaaccat tgccgaaggc gaggtgatgc aacttgccgc ggcaaagaac acggcgacca
     1801 ccgaggacga atatctcgcg gtgatccgcg gcaagaccgc ggagttgttt gccgccgcct
     1861 gcgaggtcgg acctgccatc gccaaccggc cgaaggcgga gcagaccgca tgccgctcgg
     1921 tcggcatgaa tctcggcatt gcattccagc ttgtcgacga cgtgctcgac tatggcggca
     1981 aagccgccaa gctcggcaag aacatcggcg acgatttccg cgagggcaag atcacgctgc
     2041 ccgtggtgct ggcgttccgc cgcggcaacg acaccgagcg cgcattctgg atccgcgcgc
     2101 tggagcgcgg ggacatcggc gatggcgatc tcgagcacgc catcggcttg atgaccaagc
     2161 accgcgcgct ggaagacacc atgaaccgcg cccagcacta tggcgcgatg gcagtagacg
     2221 cgctggctct gttccccgct tctgcgatga agagcgcgct ggagcaggtg gtcgcgttct
     2281 gcctggcaag atcgcattag aagcgatccc gagcggcctc agggttcgag acggcgcaag
     2341 cgcctcctca ccatgaggga tcgtcagctt agtacgcccg catgcaccca ccaatcaggg
     2401 tggtcgcgcc tgatcttgtc agcggcagcc agcgcctcgc cgccattgcc gtagatcgca
     2461 aagcaggtcg cacccgatcc gctcatccgc gcgagcagcg cacctgcact ttcgcgaagt
     2521 gccgccagca cctcgccgat cgcgggctgg atgcgcatgg cgggagcctc gagatcgttg
     2581 ccgaccaacg acagctcggc aacccaatcg gcgaccgaag cgccgcggtc cggccagtcc
     2641 ggcgactcga tcacgtcggt ggctccgacc agcagctcgc cactgcgcaa gccgagcgcg
     2701 gaaaacacgt ccttggtcgc caccgcgacg caggggttga ccagcaggca aggaagttca
     2761 ggcgggttca acggcagaag gctttcgccg ataccggtca tgtcgcaggc atgggagcgc
     2821 agacagaccg gcacgtcggc gccggtttcc agcgcaacct ccttgaggcg ctcgtcatcg
     2881 agcgggagat cgttgagctg cgccaacaac cgcagggcgg ctgctgcgtc cgccgagccg
     2941 ccgccgatgc ctgcagcgac cggcaggacc ttttccagcg cgaatgttcc aagcttcagg
     3001 ttcggcacac gtgcagccag cagcccggac gccttcagca cgagattgtc agcgatctcg
     3061 ccgcaggcct gcgccagcgg acccgtgatt tcgagtttga gatcggggcc ggggacgagc
     3121 gtcaggcgat cggcgcagtc ggcaaacgcc accacgcttt ccaggtcgtg atagccgtcc
     3181 acacggcgtc ccaccacgcg gagggtcagg ttgaccttgg cgcgtccaac ttcactcagc
     3241 atcggcgagg agcccccagc tcgcagcact agccgccctt gttaccctct tccttgccgt
     3301 cctctttctt tttgtcggca gacgccgcgg acgaagaaga cggatcgtcc ggcaagccgt
     3361 tctcgatctt ggcctcgatc ttcggcaagt cttccggctc gggtttcagg tcgcgggcat
     3421 gggaccactg gaatttggcc tccagcgtac ggccgacgcg ccaataggca tcgccgaggt
     3481 gatcgttgat ggtgggatcc tcgggcttca gatcgatcgc gcgctcgagg ttcttcaccg
     3541 cctcgtcgaa attgccgatg cggaagtaag cccagcccag cgaatccacg atatagccgt
     3601 cgtcgggacg ctgctcgacg gcgcgcttga tcatcttcat gccctcgtcg aggttgacgc
     3661 cctggtcgat ccaggaatag ccgagataat tgagcacatg cggctgatcg ggctgcaact
     3721 cgagcgcctt gcgcatgtcg gcttccgcct tgggccactg cttggaacgc tcctcgcaaa
     3781 tgccgcgata gtagtaatag acgctgttgg atttgtcgtt gccctcaggc agtacgtcga
     3841 tgcccttcga ataggtttcg gcgcaatcgg cgaatttctt gcgtccgcgc tcgatattgc
     3901 cgagcgccat gatggcttcg agatctttcg gatcgtccgc gataacgccc ttcaagatct
     3961 tgatcgcctc gtcgccgcgg tcgacgctgt cgaggtcggt ggctagctgg atctgcgcgt
     4021 tgcgcttcag cggcgaaccc gccggcacgc gctcgtagac cttgatcgcc atctgcggct
     4081 ttttcaccga ttcgtagaga tcggcgagcg aaagcagcgc cagcggatga tttggcgcaa
     4141 gatgaagcgc gagctgcaga tagaccagcg cgaggtcttc gccgccgcgc cggatcagcg
     4201 tggcgccgat gccgtagagc gcttcggctg cacccgcctg cgcggaatcg accagcggcg
     4261 gcatcttctt gccggccttg gtgtcgcgga gaccctccag caccagtgga tggcgcgcga
     4321 gcttcttgtc gaaggcttcg tagattgcgg tcgcctgcgc ttcgcccttg ttgcgcgaca
     4381 gccagcgcgc ataggcttcg ttcacgcgca gcatggaatc gtcaagcttg taggcgcgct
     4441 cgaagcgcgc gccggcatcc ttctcgcggc ccgcctgctc cagaatcatg ccggcgtgga
     4501 ggtccttgaa gatcggatac cattcgggcc cggtcagctt gtcgatattg gcgatcgccg
     4561 tcttggtgtc gccggcacca tagctcgccc acgccgacag cagcgtcgcg accaggtcgg
     4621 tgatcggacc gcggatcgac tggttgatgt tcaactgcgc cgcggcatat ttcttcagct
     4681 tcaggtcctg cacgccgacc acaagccgcg cgacgcggtt ggacttgtcg acggtcagga
     4741 tgcgctcgcc gagcttgacc gcctcgtcga tgtcgccatc ggcgaccgag gagatgaagg
     4801 cgcgatcgag cagttcgttg ttcttcggat ccgtgcgcag cgccgaacgg tagaacgcgg
     4861 cggccgaggt cgcatcccgc tccac
//
LOCUS       DATLJT010000346         4960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2547390,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000346 DATLJT010000000
VERSION     DATLJT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547390"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_08025"
     CDS             complement(<1..230)
                     /locus_tag="VM910_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62521.1"
                     /translation="MRPPRVRFWWQPIPKAKNVVLIHGLFADGSCWSEVIARLQAKGI
                     QATAVQNPLTTLQAAVEATQQILAWQQGPTVLV"
     gene            complement(675..833)
                     /locus_tag="VM910_08030"
     CDS             complement(675..833)
                     /locus_tag="VM910_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62522.1"
                     /translation="MNGGVVPINGGQATVANTATIQVLGQGGNDTIALEESNGALPAA
                     QLFGVARF"
     gene            982..3075
                     /locus_tag="VM910_08035"
     CDS             982..3075
                     /locus_tag="VM910_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI62523.1"
                     /translation="MKRSDSRRLFDKVVARQRHQFGLSNEGGALNFRFANFEIDLARQ
                     EMRRTGAIVHVEPQVFDLLVHLIRNRDRIVSKDELFEAIWQGRIVSEATLSSRISAAR
                     RALGDSGNDQTFIRTLHKRGFRFVGDVECDSSAPAANAIETRIALEDPVHETAKLLPA
                     PASLPTSDEPSAAALSFDDVGRDSDQEYLAHGLAAAVASRPVVADRVSDAADDFPMLH
                     ADPGAAPAAQTKAAASNGRRITRNLLVTGGAVALVSLTALAAWWLLLSPSTPYVKDPA
                     AVASEVASSEDRLNAWTPSIVVLPFANLSGDPKRDYLADGITDSLISDLAHALPGVPI
                     VSRDTAFTYKGRGADAREIGRELEVRYLLEGSVVLDGERVRVNTRLVETKEASQLWAE
                     RFDAELKSILQVQDEIVSRVSRAIGLLVVDIEARRSWRERPDSAELIDLVMRGKAVLN
                     LPSSPATMIEARGLFEQALKVEPASVDGLAGVATTLVFEFLNGYYETGGDERLGRAER
                     LLNRALAIEPRHLMTLKANAALRRAQGRFDDAIDMARTVIMENPGEPWTYKEIGLSTL
                     YLGKPEQALEWFAKADRIGPRDPGRWTWLDGRGHALVLLGRDEEAIRALVNALDANPK
                     NISPHAFLAAAYALLGRSEEARAALAAYLERRPGSRVSTFRMQSPVPLALTSTAYRQL
                     FARVHEGLRKSGMPE"
     gene            complement(3483..4457)
                     /locus_tag="VM910_08040"
     CDS             complement(3483..4457)
                     /locus_tag="VM910_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62524.1"
                     /translation="MRAFVKRRELLLGLAALPVAARIAVAETYPSRAVRILVATSAGG
                     GTDLVARFIAQWLTERLGQSFFVENRPGGGNNIGTEMAARSPADGYTLFMANTVNTIN
                     NSLYQKLNYNFIADFAPVANVMGTPLLVLVRPPLAAKNAAELIALAKANPGKLNLASG
                     GVGSTGHMSAELFQMMAGIKFTHVPYRGEAPALTDLIAGQAELMVSTTGSSLQYAKAG
                     AVRALATTTDDPVADLPDVPPLSKTVPGYRADAWSGLCAPKGTPSEIIALLNKEVNLA
                     MADPKIKERIASLGGVALPGSPQDYGRTIAADTEKWAKVVQFSGAHVN"
     gene            complement(4481..>4960)
                     /locus_tag="VM910_08045"
     CDS             complement(4481..>4960)
                     /locus_tag="VM910_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62525.1"
                     /translation="RTAALIMCGTGYNPKKEFIPPRIEAYKEHGIGYRWGYTFEDFSP
                     AFRATPMAHFFADLFADRNVFGDVDTIITQFEAYRQPEPEGHHTKVACPAIIITGSED
                     NTHRTAFALQKRIAGCELKIIYGAGHACQIEQPALFNRYMIEFLTAHGLFPGAARRG"
BASE COUNT          898 a         1516 c         1607 g          939 t
ORIGIN      
        1 accagaactg tcggcccttg ctgccaggcc aaaatctgct gtgttgcctc gacggccgct
       61 tgcagcgtcg tcagcggatt ctgcactgcc gtggcctgaa taccctttgc ctgcagccgt
      121 gcgatgactt cggaccagca cgacccgtcc gcaaaaaggc cgtgtatcag gacgacattt
      181 ttcgccttgg gtatcggctg ccaccagaag cgaactcgcg gcggccgcaa ctgcggtact
      241 cacaaaagta cgtcgatcca tcggaatctc ctttgccgct gggattcgta tcacgttcga
      301 tgcggcttcc ccgcttcggg gtgggctttg aagaaagcca aaaagcttcg aaacttgggg
      361 tttggatggt acacgagtgc agctccacgt gcttcagacg tccaggagat tctccaccgt
      421 ggaaaagcga gatcaagtca atggttcgcg gtctgacatg actcgtctat gctcgcgggc
      481 actttgggtc gcgaatgacc cactgcggtc attgatttcc gcaagcgcgc aatttttttt
      541 cgcgcaacgc atgcagttcg gcctcaagtg aaaagaagcg gcttagaaga cgtcgctcat
      601 gcaatgctgc attgtcagcg gatgggagaa gtagatctcg gcgtgaacga ggacaagtgt
      661 tgcggcaaac atgatcaaaa tcgcgctacg ccgaacagtt gggcggcggg cagcgcgccg
      721 ttcgactcct cgagcgcaat cgtgtcgttg ccgccctggc cgagtacctg gatcgtggca
      781 gtgttggcga cggtggcttg accgccatta atgggcacca caccgccgtt cacgaaaagt
      841 tgtccagccg cgtcgcgacg agtcgcgata gtgctatcga cgccgtcgcc tatttcgaaa
      901 cttgacccgc ggttccagtc cgtcttgaat ctcgctggat tctctttggg aatttcctga
      961 agattttctg aaaccggtag cgtgaaacgt tccgatagcc ggcggctatt tgacaaagtc
     1021 gtcgcgcggc agcgacacca gtttggcctg agcaacgagg ggggcgcttt gaacttccgc
     1081 ttcgccaatt tcgagattga cctcgcacga caggagatgc ggcgcaccgg cgcgatcgtt
     1141 cacgtcgagc cgcaggtgtt cgacctcctt gttcacctca ttcgcaaccg cgatcgaatc
     1201 gtgagcaagg acgagctctt cgaggcgatt tggcagggac gcatcgtctc ggaggcgacg
     1261 ctgagctccc gcatcagcgc tgctcggcgc gcactcggcg acagcggcaa cgaccagact
     1321 ttcattcgca cgctgcataa gcgtgggttt cgtttcgtcg gtgacgtcga atgtgatagc
     1381 tctgcgccgg ccgctaacgc gatcgaaaca agaattgcgc tggaagaccc ggtgcatgag
     1441 acggcgaagc tgctgcctgc tcctgcatcg ctgccgacct ccgacgaacc ctcagctgca
     1501 gctctctcgt tcgacgacgt cgggcgcgat tcggaccagg aataccttgc ccatggtctc
     1561 gccgcggccg tggccagccg gccggtggtt gccgatcggg tatcggacgc cgccgacgac
     1621 tttccgatgt tgcacgctga tccgggtgcc gcccctgccg cccaaacgaa agcggcagca
     1681 tcgaatgggc ggcgaattac acgaaacctg ctcgtcaccg gcggggccgt tgccctcgtt
     1741 tcattgactg cgcttgcggc gtggtggctg ctgttgtcac cctccacacc gtacgtcaaa
     1801 gaccccgctg ccgtggcaag cgaggttgcg tcctcggaag accggctcaa cgcgtggacg
     1861 ccatccatcg tcgttcttcc atttgcgaat ctcagcggcg atcccaagcg ggactacttg
     1921 gccgacggaa ttacggacag cctcatcagc gatctcgcgc acgcgctgcc cggggtcccc
     1981 atcgtctccc gtgacacggc gttcacctac aagggccggg gtgcagacgc gcgggagatc
     2041 ggccgcgagc tggaggttcg ttatctgctc gagggcagcg tggtcctcga cggcgagcgc
     2101 gtgcgcgtca acacgcggct ggtcgagacc aaggaggcga gccagctgtg ggccgagcgt
     2161 tttgacgcgg agctgaagag cattctgcag gttcaagacg agatcgtcag ccgggtttcg
     2221 cgcgcgatcg gattgctggt ggtcgacatc gaagcacggc gaagctggcg cgaacggccg
     2281 gacagcgccg aactcatcga cctcgtcatg cgcggcaagg cggtgctcaa cctgccgtca
     2341 tcaccggcga cgatgatcga ggctcgcggt ctcttcgagc aggcgctcaa ggtggagccg
     2401 gccagcgtcg acggcctggc tggcgtggcc acgacgctcg ttttcgaatt cctcaacggc
     2461 tactacgaaa ccggggggga cgagcggctc ggcagggcgg agcggctgct caatcgcgcg
     2521 ctcgcgatcg agccccgtca cctcatgact ttgaaggcga acgccgcgtt gcgccgcgcg
     2581 cagggcagat tcgatgacgc catcgacatg gccagaacgg tcatcatgga aaatcccggt
     2641 gagccgtgga cctacaagga aatcggcttg tcgaccttgt atctcggcaa gccggaacaa
     2701 gcgctcgaat ggttcgccaa ggccgatcgc atcggcccgc gcgacccggg acgctggacc
     2761 tggctggacg gccgcggcca cgcgctcgtc ctccttgggc gggacgaaga agccatccgc
     2821 gcattggtca acgccctcga tgccaacccc aagaacatct cgccacatgc ttttctggcg
     2881 gcagcctacg cgctcctcgg tcgctccgag gaggctcgcg ccgcgcttgc ggcctatctg
     2941 gaaaggcggc ccggcagtcg agtgtcgaca ttccgcatgc agtcgccggt tcccctcgcg
     3001 ctcaccagca ccgcctatcg gcagctgttc gcgcgggtcc acgaggggct gcgcaagagc
     3061 ggaatgcccg aatagttgcc tgtgatcttg gtccggcgag cggcgccgtt cttcccgaca
     3121 cgtgtggggc cgttttggcc tattcagctc cgcgggctgc gagacgggaa ggtgtgcacc
     3181 agcggcattt ggcgttcgcg tcgatccagc tcatgacgag cctcgcagtg gctcgaacaa
     3241 aagcgaacgt gaatccacca gcgtccggtc cacagattac gggagcgcac acctaacccg
     3301 agtcttccgt gacattgggc gcatcttttc attgctgttc ctactcagcc acccgtcgcc
     3361 cattggtcac ccgctgagaa gaagggttca aacgcgggaa cgggaatatg gtttcgtcga
     3421 gggaaaaaat ggtttcaatt gcgtttcgtg tggaactcgc cgccccgggg cacaagagcg
     3481 attcaattca cgtgcgcgcc ggagaactgc acgaccttgg cccacttctc ggtgtcggcc
     3541 gcgatagttc ggccgtagtc ctgcggcgag ccgggcagcg cgacaccgcc gaggctggcg
     3601 atgcgctcct tgatcttcgg atcggccatc gcgaggttca cctccttgtt gagcaaggcg
     3661 atgatctccg aaggcgttcc tttcggcgcg cagaggccgc tccaggcgtc ggcccgatag
     3721 cccggcaccg tcttcgacag cggcgggaca tcgggcaggt cggcgacagg gtcgtcggtc
     3781 gtcgtcgcga gcgcgcgcac cgcgcccgcc ttggcgtact gcagcgagga gccggtggtc
     3841 gaaaccatca gctccgcctg cccggcgatc agatcggtga gcgcgggcgc ctcgccgcga
     3901 tacggcacat gggtgaactt gatgccggcc atcatctgga acagctcagc ggacatgtga
     3961 ccggtcgaac cgacgccgcc ggaggcgagg ttgagcttgc ccgggttggc cttggcgagc
     4021 gcgatcagtt cggcggcatt ttttgccgct agcggcggac gcacgagaac gagcagcggc
     4081 gtgcccatga cgttcgcgac cggcgcgaag tcggcgatga agttgtagtt gagcttctga
     4141 taaagcgaat tgttgatggt gttgacggtg ttcgccatga acagcgtata gccgtccgcg
     4201 ggcgaacgcg ccgccatctc ggtgccgata ttgttgccgc caccgggccg gttctcgacg
     4261 aagaaggatt gcccgagccg ctcggtcagc cattgcgcga tgaagcgcgc gacgaggtcg
     4321 gtgccgccgc cggcggaggt cgcgaccaag atgcggaccg cacgcgaggg atacgtctca
     4381 gcgacggcga tgcgcgctgc cacgggcagc gcggcaagac cgagcaggag ttcgcggcgc
     4441 ttcacgaatg ctcgcatcgg aggcacctcc ccgacgaagc ttagccccgg cgtgcggctc
     4501 cggggaacag gccgtgggcg gtcagaaatt cgatcatgta gcggttgaac agcgcgggtt
     4561 gctcgatctg gcaggcatgg ccggcgccgt agatgatctt cagttcgcag ccggcgatgc
     4621 gcttctgcag cgcgaaagcg gtccggtgcg tgttgtcctc gctaccggtg atgatgatgg
     4681 ctggacaggc gaccttggtg tgatggccct cgggctcggg ctgccgatag gcctcgaatt
     4741 gggtgatgat ggtgtcgaca tcgccgaaca cgttgcgatc ggcgaacagg tcggcgaaga
     4801 aatgggccat cggtgtcgcg cggaaagcgg ggctgaagtc ctcgaaggtg tagccccaac
     4861 ggtagccgat gccgtgttcc ttataagcct cgatgcgtgg cgggatgaac tccttcttcg
     4921 ggttgtagcc ggtgccgcac atgatcagcg ccgcggtccg
//
LOCUS       DATLJT010000347         8198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3925226,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000347 DATLJT010000000
VERSION     DATLJT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925226"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1001)
                     /locus_tag="VM910_08050"
     CDS             complement(282..1001)
                     /locus_tag="VM910_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI62526.1"
                     /translation="MENQDLVVVAVVTLAGVMRGITGFGGAMLMAPPLSTTLGPVPAV
                     VTALTLEAAAALVMFPDAWPRINRRILVYLTAPAVLTVPIGGYFLVNVDAFVARKVIA
                     AVVVIFALALLCGWRYSKPPRPATSIALGSIVGLLLGATSVGAPPVILYLLASSDSQA
                     VTRANLTVFVTAISGIGLVMLLAIGAVTAPLAAYAGIMIIPFLLATWLGGKLFGRMNG
                     SSVRAAALGLMLFVGTVGLLV"
     gene            complement(1010..1768)
                     /locus_tag="VM910_08055"
     CDS             complement(1010..1768)
                     /locus_tag="VM910_08055"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62527.1"
                     /translation="MDLDLKGRVALVTGAAAGIGEAIARALAGEACSVWLLDRDRGGA
                     ENVAGALTSTGHRATALVVDVANHDAVSAVFAEIVQRTGRLDILVNAAGLLSTGLVAD
                     LPPSEWDRVSRVNVSGIINCVKAALPSMAAQRRGRIINIASISAMRGGGSIGNTIYGA
                     TKASVVALTKGLARELGPQGITVNAVAPAIADTAMTHAALTAEARQRILLRIPLGRLA
                     APSDIADMVAFLASDRAAFITGTTIPVDGGILTT"
     gene            complement(1794..3155)
                     /locus_tag="VM910_08060"
     CDS             complement(1794..3155)
                     /locus_tag="VM910_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62528.1"
                     /translation="MQSARTQDGGSGTSIGKVAFASAIGTTIEWYDFFLYGVVTPIVL
                     NKLFFPNFDPVVGTLLAYTTFFVGFLSRPIGGIIFGHYGDRIGRKTVLVLTLLIMGLA
                     TFLIGLLPTYASAGIWAPIMLLVLRIFQGIGIGGEWGGAVLMAVEHSPQGKRGFYGSW
                     PQVGVPAGLLLSSAFVYLLSFLPENDFYSWGWRISFLISVVLVAIGLYIRLKIMETPA
                     FTRLQQTQEVAHVPFFELLRTHGWNTLLGMGARYIEGVTFNIFGVFIIAYVTGALQLP
                     RQTALAGVMISSAIMLVMLPIYGNLSDRIGRRRLFAFAGLLIGILSFPSFWLMETKSS
                     LLIWIAITVPFALVYPAVYGPQAALFSELFDTRVRYTGISFVYQFSAIFASGLTPIIA
                     TSLLPLGGNKPWLICLYILAVSIISALSVFAMSDMHKRDIVADGVIQGLQTTEAKMTR
                     SAL"
     gene            complement(3188..4018)
                     /locus_tag="VM910_08065"
     CDS             complement(3188..4018)
                     /locus_tag="VM910_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVI62529.1"
                     /translation="MPQPAIITVAITGAIPRKVDTPAVPVTPSEQIESTHEAFEAGAS
                     VVHIHVRDPDESPSSDPTLFAQVQEGVSKHCPGMIIQFSTGGRGREQSERGKMLYLAP
                     DMASLATGSVNFPNQIYENPPQLVEELARTMLTYNVKPEIEVFDLAMLYNAKVLADRG
                     LLARPCHVQFVMGIPNALPARRNLLEFLVSELKELMSDATWAATGIGRHQLEVNEWCL
                     ALGGHVRTGLEDNIKFDRTRLARSNAELVSRLAGMCAAYARYPATSVEARRILQLPPA
                     "
     gene            complement(4020..5471)
                     /locus_tag="VM910_08070"
     CDS             complement(4020..5471)
                     /locus_tag="VM910_08070"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HVI62530.1"
                     /translation="MAKDRTIQLVDVTLRDAHQCLWATRMTTAMMAELAPRLDLAGFE
                     AIDLVGGAVFDVCVRYLREDPWERMRIVSGWVKNTPLIVMTRGQSLFTFEFFPDDIVE
                     LTAERIFANGIRYHTPYDALNDMRNMEVPVRAAKRVGLYTAGGIVYTVSPVHTDKYYA
                     HKARELIALGVDAVYIKDPSGLLTPERAVTLVSAIKTACGKLPLHLHSHCLTGLAPYT
                     ALRSIELGVDVVHTATSTLANGASHPATENFVQNARRNGFTVPVDLAPVAEVAERLRY
                     IATRENKPIGEIAEHDAFHYEHQIPGGMVSNLKSQLVPLGIAHRLPEVLEEASRVRHE
                     LGYPIIVSPFAQFVMTQAVLNVVGKERYGTVPDEVRKYVLGYYGEIAGPIDPNLYDRI
                     TNGAEPVKSRPGDLLGSGIPKLRRERGPFASNDDLLLAAFYSRSEFDALKAAGPIKTT
                     YQLGATPLITLIKEVAQRRDIRTFHFLQRTQAS"
     gene            complement(5526..6707)
                     /locus_tag="VM910_08075"
     CDS             complement(5526..6707)
                     /locus_tag="VM910_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVI62531.1"
                     /translation="MRRVLIATRGEIALRAVRACRRLGLQSVAVHSSSDGNSPHVCAA
                     DHAVCIGPPPPPASYLNAAALIETAIALKCDAIYPGYGFLSERSDFAAKCSEAGLTFV
                     GPNEETIACMGDKVAARRTAASQGVPIVPGSERGFTDAAEAVSAGQEIGFPLLLKANG
                     GGGRGIRIAERPQDFAFEQASAEAWGAFGRRDIYLERFFPEVHHVEIQVFGDIHGNYV
                     HIWERDCSVQRRHQKLVEEAPSPVLDHATRRDMADAAVALIRALKYVNAGTVEFIYDR
                     ASGKFYFIEMNTRTQVEHPVTEMLTGTALVAEQLRIAAGEPLSVKQSPANCRSAIEFR
                     INAEDVDQGFRPSPGTLSRWRPPKERDIRIDTHVYEGYAIPPYYDSYSVSRTSSPTSG
                     I"
     gene            6820..7713
                     /locus_tag="VM910_08080"
     CDS             6820..7713
                     /locus_tag="VM910_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVI62532.1"
                     /translation="MSRARATPIQLPSNWPPSPKARGRLGRSKGASRAYPTIKAVTIL
                     EAMTSARRPLSISELSVSLGIPKPTAHRIIRMLEEERLLQREPGERRFIPGSRLVSLG
                     LDIVAASMQTAPRHAILAALSAEIGETCNFGVLAGSHVAYLDRVESAWPFGLRFEPGS
                     RVPLHCTSMGKLFLSLLPKQKRAALLRATPLYRYTENTITNLEALDAELDRIRAAGVS
                     TDNQEFLAGVVCVAVPVSTASRQPAAALAVSAPTARMSLQQALEYLPSLRAAADRLAE
                     TIVGAEGTPAGRRRKSRGNVA"
     gene            7710..8198
                     /gene="accB"
                     /locus_tag="VM910_08085"
     CDS             7710..8198
                     /gene="accB"
                     /locus_tag="VM910_08085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVI62533.1"
                     /translation="MTLRYEEIAEILRIIDSSSCDEVVIETAEVKLVVRRNSTAGPAD
                     LAPPERPTAPLAAATDSLSQLEPAAPPSAGAKIAVTGGQFVVTAPMVGTFYRQPSPDA
                     PPFVEIDSVVRKGQPLCLIEVMKLFTTIHAEQDGRIVQIGANNGELVEYGQTLFVIEP
                     LA"
BASE COUNT         1676 a         2492 c         2471 g         1559 t
ORIGIN      
        1 gctctcccgg tcccggtatt cggcccgcga atgctaacgg tcaagcacgt gatgatcgcg
       61 gtgagcgtcc caataccctt gcataccttc cgtttgatct aatttgcggt cagttcgaag
      121 gcgcaggtgg cgggcgttaa ggtacttgcc ttcgccgtgt cgtggccgct gcatgctaat
      181 cacagcgtga agccgtccca ttccggttct gcgtcgcagc actgctgcgg tgtgccctgc
      241 gtggcaggtt cgccgtgact atagcttagt gaatcacgtg cttacaccaa caagccgaca
      301 gtgcccacaa acagcatgag accaagcgca gccgcacgca cgctggagcc gttcattctg
      361 ccgaacagct tgccgcccag ccatgttgcc agcagaaacg ggattatcat gatcccggca
      421 taggcggcga gcggtgccgt gaccgcaccg atcgcgagca acatgacgag tccaatcccc
      481 gaaatagcgg tgacaaagac cgtcaaattt gcgcgggtga cggcctggct atcggaacta
      541 gccagcaagt acaggatcac cggcggcgcc ccgacgctcg ttgcgccaag taaaaggccg
      601 acgatgcttc ccagcgcgat agaggtcgca ggccttggag gtttggagta gcgccagccg
      661 cacagtagcg ccagcgcgaa gatgacaacc acggcggcaa tcacttttcg cgcaacaaac
      721 gcgtcgacgt tcacgagaaa gtagccccct atcggaacag tcaggactgc gggcgctgtc
      781 agataaacta gtatgcgcct atttatgcgc ggccatgcgt ccggaaacat aacgagagct
      841 gccgccgctt ccaacgtcaa cgcggtcaca accgcgggaa ccggaccgag cgtagtactc
      901 aacggcggcg ccatcagcat ggcgccccca aatcccgtaa ttccacgcat gactcctgca
      961 agcgtgacga cggcgacgac aacgagatcc tggttctcca ttctgggaat caggtggtca
     1021 aaatgccgcc atcgaccggg attgtggtcc cggtgatgaa tgccgctcgg tcggaggcca
     1081 agaaggccac catgtcggcg atgtcggatg gagcggcgag gcggccgagc ggaatccgta
     1141 acagaatccg ctggcgcgct tccgcagtca gcgccgcatg agtcatcgcg gtatcggcga
     1201 tggcgggtgc cacggcattg accgtgatgc cctgcgggcc gagttcgcgc gcgagccctt
     1261 tcgtcagcgc aacgacactt gccttggtgg caccgtaaat cgtgttgccg atcgagccgc
     1321 cgccccgcat cgccgatatg gaagcgatgt tgatgatgcg cccgcggcgc tgggcagcca
     1381 tggagggcag agccgctttg acgcagttga tgatgccgga gacgttgact cgcgacacgc
     1441 gatcccactc ggagggcggc aaatcggcaa ctaggccggt cgagagcagc cccgccgcat
     1501 tgacgagaat gtcaagccgt cctgtgcgtt gcacaatttc agcaaacaca gctgacaccg
     1561 cgtcgtgatt ggcgacgtcc acaacgagag cggtggcccg gtgaccggtg gacgttagcg
     1621 cgcctgccac attctcggcg cccccgcggt cccgatcgag aagccacacc gaacaggcct
     1681 cgccggccag tgcgcgcgca atcgcctcac cgatgccggc ggctgcgccg gtcaccagcg
     1741 caacacggcc tttcaggtcg aggtccatgg tcagaccgca tcgtcgggtc gatctagagc
     1801 gcggaacgcg tcatctttgc ctcggtcgtc tgcaagccct ggatgacgcc gtcggcgacg
     1861 atatcgcgct tgtgcatgtc gctcatcgca aacaccgaca aggcactgat tatgctcacc
     1921 gcaaggatat agaggcagat cagccaaggc ttgtttccgc caagcggcag aagtgaagtg
     1981 gcaatgatcg gcgtcagtcc gctcgcgaag attgccgaga actgatagac gaacgagatc
     2041 cccgtgtacc gcacgcgggt gtcgaacagt tcggagaaca gcgcggcctg aggcccgtag
     2101 accgccgggt agaccagcgc gaatggcacc gtaatcgcga tccagatcag cagcgacgat
     2161 ttggtctcca tcaaccagaa ggaaggaaac gacaggatgc caatcagcaa tcccgcgaat
     2221 gcgaagagac ggcgtcgccc gatgcggtcc gacagattgc cataaatcgg cagcatgacg
     2281 agcatgatcg ccgacgagat catcacgccg gcgagcgccg tttgtctcgg caactgcaac
     2341 gctccagtga cataggcgat aatgaagacg ccgaagatgt tgaacgtcac cccttcgatg
     2401 taacgggcgc ccatccccag cagcgtgttc cagccgtgag tccgtagcag ttcgaagaac
     2461 gggacgtgag caacttcttg cgtctgctgc aggcgtgtga atgcgggcgt ctccatgatc
     2521 ttgagtcgga tgtagagccc gattgcgaca agcacgacgc tgattaggaa cgaaatacgc
     2581 caaccccagg agtagaagtc gttctccggc aaaaatgaca gcagatagac gaacgctgag
     2641 cttagcaaca gccctgcagg cacgccaacc tgaggccagc ttccatagaa tccgcgcttg
     2701 ccctggggcg aatgttccac cgccatcagc acggcgccgc cccattcgcc gccgatgccg
     2761 ataccctgaa atattcgaag caccagcagc atgatcggag cccaaatgcc ggcactcgcg
     2821 taggtcggca gcaatccaat cagaaaggtg gcgagcccca tgattaggag cgttagcacc
     2881 agcacggtct tgcggccgat tcggtcgcca tagtgaccaa atatgattcc gccaatggga
     2941 cgggacagga agccaacgaa aaatgtcgtg tacgccagca acgtgccgac taccggatcg
     3001 aagttcggaa aaaacaactt gttgaggacg atcggggtca cgacgccgta aagaaagaag
     3061 tcgtaccact cgatcgttgt accgatggcg cttgcaaatg cgaccttacc gatggatgta
     3121 ccagagccac catcttgtgt tcttgccgac tgcatttttc ctcccactgt gttttgccgt
     3181 cgcgctccta tgcgggcggc agttgcaata tccgtcgagc ttccactgaa gttgccgggt
     3241 atcgcgcata tgccgcgcac atgccggcca agcgtgacac aagctccgcg ttgctgcggg
     3301 caagacgggt acggtcaaac ttgatgttgt cctccagtcc ggtgcggacg tgaccgccaa
     3361 gcgcgaggca ccattcattg acttccaatt ggtgccgacc gatcccggtg gcggcccagg
     3421 tcgcgtcgga cataagctcc ttcagctcgc tgacgagaaa ttcgagaagg ttgcgccgtg
     3481 cggggagcgc atttggtatt cccatcacga attgcacatg acacggccgt gcgagcaatc
     3541 cgcggtccgc gagaactttg gcattataga gcatggcgag gtcaaacacc tcgatttccg
     3601 gcttcacgtt gtaggtgagc atcgtgcgcg cgagttcttc cacgagctgc ggcgggttct
     3661 cgtaaatctg gttggggaag ttaaccgatc cggtcgcgag tgaagccata tccggcgcga
     3721 gataaagcat cttgccgcgc tcgctttgct ctcgcccgcg ccctccagtc gaaaactgga
     3781 tgatcatgcc cgggcaatgc ttcgacacgc cctcttgcac ttgggcgaat agcgtcgggt
     3841 ccgagctcgg actttcatcg ggatcgcgca catggatgtg aaccacggaa gcgccggcct
     3901 cgaaagcctc gtgcgttgac tcgatttgct cggacggtgt gactggaacc gccggcgtgt
     3961 cgactttgcg tgggatggcg ccagtaatgg ccacggtgat gatcgctggc tgcggcatct
     4021 caactcgctt gggtgcgttg caagaagtgg aacgtgcgaa tatcgcggcg ctgcgcaact
     4081 tctttgatca gggtgatcaa cggcgtggca ccgagctggt aggtcgtctt gatcggaccg
     4141 gcggctttga gcgcatcgaa ctcggaacgg gaatagaacg cggcgagcag caggtcgtcg
     4201 ttcgaagcaa acggaccgcg ctcgcgccgt aatttcggaa tgccggagcc gagcaggtca
     4261 ccgggcctgc tctttacggg ttccgcgcca ttcgtgatcc ggtcgtaaag attggggtcg
     4321 atggggccag cgatttcgcc gtaatagcca agaacatatt ttcgaacttc gtccggcacc
     4381 gtgccgtagc gttcctttcc caccacgttg agtaccgcct gcgtcattac gaactgagca
     4441 aacgggctga caatgatcgg atatccgagt tcatgacgca cacggctggc ttcctccaga
     4501 acttctggca accggtgagc gatgccgagc ggcacaagtt gcgatttcag gttcgacacc
     4561 atgccgccgg gaatttgatg ctcgtaatgg aaggcgtcgt gttcggcgat ttcgccgata
     4621 ggtttgttct cgcgcgtggc gatatagcgc agtcgctctg ctacctcggc caccggcgcg
     4681 agatccactg ggacggtaaa gccgttgcgg cgtgcgttct ggacaaaatt ctcagttgcg
     4741 ggatgcgagg cgccgttggc gagcgtcgat gtggcggtgt gaaccacatc gacgccgagc
     4801 tcgatcgagc gtagcgccgt atatggcgcg agccccgtaa ggcaatgcga atgtagatgc
     4861 aacggcagtt tgccgcacgc cgtcttgatc gcggagacaa gcgtcaccgc acgctcaggc
     4921 gtcaataggc cgctcggatc ttttatgtag acggcatcga cgccgagcgc gatgagctcg
     4981 cgcgccttgt gcgcatagta tttgtcggta tgaacgggac tcacggtata gacgatcccg
     5041 ccagcggtat agaggccgac cctttttgcc gcacgtaccg gcacttccat gttgcgcatg
     5101 tcgttcagcg cgtcatacgg cgtgtgatag cgaatcccgt tggcaaaaat ccgctcggcc
     5161 gtgagctcga cgatatcgtc agggaagaac tcgaacgtga acaggctctg gccacgcgtc
     5221 atgacgatca acggcgtgtt cttgacccaa ccgctgacaa tgcgcatgcg ctcccagggg
     5281 tcttcccgca aatagcggac gcagacatcg aatacagcgc cgccgactag gtcgatcgcc
     5341 tcgaaaccag ccaaatccag ccgcggcgct aactctgcca tcatcgctgt agtcatccgc
     5401 gtcgcccaga ggcactgatg cgcatcgcgc agcgtgacgt cgacgagttg aatcgtccgg
     5461 tccttggcca tcactgtacc gtctgcaatt tctgctcgac ccagcgggtg tgcacctccc
     5521 cgcgctcaaa tcccgctcgt tggagaagac gttcgtgaaa cggaatagga atcgtagtag
     5581 ggcggtatcg cgtagccttc gtagacatga gtgtcgatgc ggatgtcacg ctcttttggc
     5641 ggccgccaac gactcaatgt tccgggcgaa ggccgaaatc cctgatcaac atcctcggcg
     5701 ttgatgcgaa actcgatggc ggatcggcag ttggctggtg actgcttgac cgacagcggc
     5761 tcacccgcgg cgatcctgag ctgttctgct acgagagccg taccggtcaa catctcggtg
     5821 acggggtgct cgacctgggt gcgtgtgttc atctcgataa aatagaattt gccgcttgcg
     5881 cggtcgtaga tgaactcgac ggttccggcg ttgacgtatt tgagggcgcg aatgagtgcg
     5941 accgcggcgt ccgccatgtc gcgtcgcgtc gcgtgatcga ggacaggcga cggcgcctcc
     6001 tcgacgagct tttggtgtcg gcgctgcacg ctacaatcgc gctcccatat atgcacgtag
     6061 ttgccgtgga tatcgccgaa aacttggatc tccacgtggt gaacttccgg aaagaagcgt
     6121 tccaaataaa tgtctcgacg gccgaacgcc ccccacgctt cggccgacgc ctgttcgaag
     6181 gcaaaatcct gcggccgttc ggcaatgcga atgccacggc cgccgccgcc gttggccttc
     6241 agcaatagcg gaaagccgat ctcttgcccg gccgatactg cctcggctgc gtctgtgaag
     6301 ccgcgttccg aacccggcac gatcggcaca ccttgcgagg ctgcggttcg gcgcgccgcg
     6361 actttgtcac ccatgcaggc gatcgtctct tcattcggtc ccacgaatgt caggccggct
     6421 tcgctgcact tggcggcgaa gtcactcctt tccgacagga agccgtagcc gggatatatg
     6481 gcgtcgcatt tgagcgcgat cgccgtctcg atcagggcgg cagcattcaa ataactggca
     6541 gggggtgggg gcgggccgat gcagactgcg tgatcggcgg cgcagacgtg cggtgaattt
     6601 ccgtcactgg aggaatgaac cgcaaccgat tgaagtccaa gtcgccggca cgcgcgaacg
     6661 gcacgcaaag caatctcgcc gcgagtggcg atgagaaccc gccgcaccgg cctctcctct
     6721 ggctgtcgct aggaccctct tgcggttgct tccatctgcc actttcggat acaaaaattc
     6781 atattgcaag atgttttgtt ccggtttttg atacaaccta tgagccgtgc ccgcgctaca
     6841 ccaatccagc tccccagcaa ttggccgcct agtcctaagg cgaggggacg gctggggcgc
     6901 tccaagggcg cctcgcgtgc ctatccgacc atcaaagcag tgacgatcct cgaggcgatg
     6961 acgagcgctc gccgcccgct aagcatctct gagctcagcg tgtcgctcgg tatccccaag
     7021 cccactgcac accgcatcat taggatgctg gaggaggaac ggctgctgca gcgggaaccg
     7081 ggcgagcgcc gtttcatccc tggctcccgt ctcgtcagcc tcgggctcga catcgtcgcc
     7141 gcatccatgc aaacagcacc gcgccacgcc attctggcgg cactgtcagc cgagatcggc
     7201 gagacgtgta atttcggcgt gctcgccggt agccatgttg cttatctcga tcgcgttgag
     7261 tcggcgtggc cattcgggct gcgattcgag cctggatcgc gcgtgccgct ccattgcacg
     7321 tcgatgggca agctgttctt aagcctgctt cccaagcaga agcgagcggc gctgctgcgt
     7381 gcgacgccgc tctaccgcta taccgaaaat acgatcacca acctggaggc gctcgacgcc
     7441 gagttggata ggatccgagc agccggcgtc tctacggaca accaagaatt tctcgccggg
     7501 gtcgtttgcg ttgccgtccc cgtctcgacc gcaagtcggc agccggcggc cgctctggcg
     7561 gtgtcggcgc caacggcgcg catgtccttg cagcaggctc tggagtacct tccatcgctg
     7621 cgagcggcgg ccgaccgcct agccgagacc atcgtcgggg ccgaagggac ccctgccggt
     7681 cggcggcgca agtcacgcgg taatgtcgca tgacgttgcg ctatgaagaa atcgccgaga
     7741 tcctcaggat catcgatagc agcagttgtg acgaggtcgt tatcgagacc gcagaggtca
     7801 aactcgtcgt tcgccgcaat agcacagccg gcccagcgga cttggcgccg cccgaaaggc
     7861 cgactgcgcc actggccgcc gcgacggact cgctctctca gctcgaacct gcagcgccgc
     7921 catcggccgg cgcgaagatc gccgtcaccg gcggccagtt tgtagtcacc gcgccgatgg
     7981 ttggaacgtt ctaccgtcag ccctcgcccg acgcgcctcc tttcgtcgaa atcgacagcg
     8041 tggtgcgcaa aggccagccc ctatgcctga tcgaagtcat gaagttgttc accacaatcc
     8101 acgccgagca agatgggcgc atcgtgcaaa tcggcgcgaa taacggcgag ctcgtcgaat
     8161 acggacaaac tttgttcgtt atcgaaccgc tcgcctaa
//
LOCUS       DATLJT010000348         4950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_708647,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000348 DATLJT010000000
VERSION     DATLJT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708647"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VM910_08090"
     CDS             <1..328
                     /locus_tag="VM910_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI62534.1"
                     /translation="YPEIRASNASIRSFNERAAINARLQGSAADIIRRAMTRMEDALA
                     EKKLSAQMLLQVHDELIFEVPDNEVAATLPVVQRVMQDAPFPAVALSLPLQVDARAAD
                     NWDEAH"
     gene            526..1161
                     /locus_tag="VM910_08095"
     CDS             526..1161
                     /locus_tag="VM910_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVI62535.1"
                     /translation="MPHTSSLLGFALISLGLVLTPGPNMIYLISRSITQGPAAGIASL
                     GGVALGFVFYMLCAAFGITALLFAVPYAYDALRFAGAAYLLWLAWQAVKPGGRSPFQV
                     RKLAKDGPRKLFAMGFVTNLLNPKIAMLYLALPPQFIDPLGSSVLAQSLVLGAIQIAI
                     SVSINAIIAHAAGSIALFLTRRPSWLLVQRYLMGTVLAGLAVRMAFEARRA"
     gene            complement(1203..1829)
                     /locus_tag="VM910_08100"
     CDS             complement(1203..1829)
                     /locus_tag="VM910_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI62536.1"
                     /translation="MTIELHTWNTPNGRKISVALEEMGLPYKVFPVNITKGEQMAPSF
                     LAISPNNKIPAIVDPEGPEAKRVSIFESGAILLYLGEKTGKFLPKPLIERIPVYEWLM
                     WQMGGFGPMPGQVHHFIALENEQDRAYGLKRYMAETRRLYGVLDRRLAGREFVAGPLS
                     VADFAILGWAWRHPRHKVELSDFPNVKRWYEMMMARPGVKRGMEAKLD"
     gene            2012..2569
                     /gene="pyrE"
                     /locus_tag="VM910_08105"
     CDS             2012..2569
                     /gene="pyrE"
                     /locus_tag="VM910_08105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVI62537.1"
                     /translation="MSKSASRARLHEIIRKRSFGRGEITLASGRKSDFYFNLKPTMLD
                     PEGATLLAELTYEALKDDELDFIGGLEMGAVPLAGAIAQISWMKGHPISAFFVRKKPK
                     EHGARLAVEGLAKGESLQGKRVVVVEDVTTTGGSAMKAVEAVREAGGKVALVLTMVDR
                     EEGATDTFARAGLPFRALYTATEFL"
     gene            2758..3507
                     /locus_tag="VM910_08110"
     CDS             2758..3507
                     /locus_tag="VM910_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HVI62538.1"
                     /translation="MVLVATLLIAGPLAFIPSPTSAEGLFDFFLGGLSKLQAHLAPPP
                     QAGSLADPFGNNQQTSAPQPMMAGSGPAYCVRSCDGKYFPLMRAAASPIQMCQAFCPA
                     SPTKVFFGASIDAAYSASGERYADSANAFAYRKALRADCTCNGRNPAGLAPVDVTLDP
                     SLREGDVVATSDGLVAYSGVRVGNDQTAEFAPVASYPGLTADLRARLGEMKVAPARAD
                     MVAGDAAPAAPATPVPPAIPVSPKAKRADLN"
     gene            complement(3516..3785)
                     /locus_tag="VM910_08115"
     CDS             complement(3516..3785)
                     /locus_tag="VM910_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI62539.1"
                     /translation="MTTMFVRHTVSNYAAWRKAYDDFAPVQKAKGVTAEAVYQAADNP
                     NDVTVTHDFATLEAARAFAGSEELKQAMQRAGVAGAPTIWFTNKV"
     gene            complement(3877..4308)
                     /locus_tag="VM910_08120"
     CDS             complement(3877..4308)
                     /locus_tag="VM910_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62540.1"
                     /translation="MSTIAFDQFALTRIADFARTLSRLHRASRTRAIDDDQIDREFNA
                     VCMSVWGYTTDDISDELFSKEDHAFLDTLDEAHARIFAALQGYDLIDDQGMLTDWWGF
                     CWMILAEKRGLLTPENRAAARVAIEEKYLSAPNVIGVIVGR"
     gene            4499..4804
                     /locus_tag="VM910_08125"
     CDS             4499..4804
                     /locus_tag="VM910_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVI62541.1"
                     /translation="MNDARSYYVDILASRHHGTLYIGITNDIRTRIEQHRAGRGSEYV
                     QKYRVFRLVHVEVFATPQEAIAREKQLKFWKRDWKIKLIEQENPDWNDRSGLIFQLG"
BASE COUNT          906 a         1558 c         1491 g          995 t
ORIGIN      
        1 ttaccccgag atcagggctt ccaacgcctc gatccggtcc ttcaacgaac gcgccgcgat
       61 caacgcgcga ctgcagggat ccgccgccga tatcatccgc cgcgccatga cccgcatgga
      121 agatgcactc gccgagaaaa aactctcggc gcaaatgctg ttgcaggtgc acgacgaatt
      181 gatcttcgag gtgccggaca acgaggttgc ggcgaccttg ccggtcgtgc agcgggtcat
      241 gcaggacgca ccattcccgg ccgtggcgct ctcgctgccg ctgcaggtcg atgcccgcgc
      301 cgccgacaac tgggacgagg cgcattagtt ctcgcgcgcg gttttgccgt gatcgtaggg
      361 tgggttgagc gaagcgatac ccaccattcg agtctcgatg ggtttcgctg acgctcaacc
      421 catcctacgg ccgtcgatat tcgaccaact gcattgcgat cttcgaaatg gtgtcgcggc
      481 gcggcgcaag ctagaagatt tgcgtcatcc tcccgcgagt cccaaatgcc ccacacctca
      541 tcgctgctcg gctttgccct gatctcgctt ggtctcgtgc tgacgccggg acccaacatg
      601 atctacctga tctcgcgctc gatcacgcaa ggtcccgcgg ccggcatcgc ctcgctcggc
      661 ggtgtcgcgc tcggcttcgt gttctatatg ctgtgcgctg cgttcggcat caccgcgctc
      721 ctgttcgccg tgccttacgc ctatgacgcg ctgcgctttg ccggcgccgc ttatctgttg
      781 tggctcgcct ggcaggcggt caaacccggc ggccgctcgc cgttccaggt gagaaagctt
      841 gcgaaagacg gcccacgcaa attgttcgcg atgggctttg tcaccaatct tctgaacccg
      901 aagatcgcga tgctctatct cgcgctgccg ccgcaattca tcgatccctt ggggagcagc
      961 gtgctggcgc agtccctcgt gctcggcgcg atccagattg cgatcagcgt cagcatcaat
     1021 gcgatcattg ctcacgctgc cggatcgatt gcgctgttct tgaccaggcg gccgagctgg
     1081 ctgctggttc agcgctatct gatgggaacg gtgctcgcgg ggcttgccgt gcgcatggcc
     1141 ttcgaggcca ggcgggcttg aacccacccc gccctccccc gcgagcgggg aagggagaag
     1201 aattaatcca gcttcgcttc catgccgcgc ttcacaccgg gacgcgccat catcatctcg
     1261 taccagcgtt tgacgttggg gaaatcggag agctcgacct tgtggcgcgg atgccgccag
     1321 gcccatccca gaatcgcgaa gtcggcaacc gacagcggac cggcgacgaa ctcccggccc
     1381 gcaagccggc gatcgagaac gccgtagagc cggcgcgtct cggccatgta gcgcttcagc
     1441 ccataggcgc gatcctgctc gttctccagc gcgatgaaat gatgcacctg accgggcatc
     1501 ggcccgaacc cgcccatctg ccacatcagc cattcgtaga cggggatgcg ctcgatcagc
     1561 ggcttgggca ggaactttcc ggtcttctcg ccgagataaa gcaggatcgc gccggattcg
     1621 aagatgctga cccgcttggc ctcgggcccc tccggatcga cgatcgccgg tatcttgttg
     1681 ttcgggctga tggcgagaaa ggatggcgcc atctgctcgc cctttgtgat gttcaccggg
     1741 aacaccttgt aaggcagccc catttcctcc agcgcaaccg agattttgcg gccattcggc
     1801 gtgttccagg tgtgaagttc gatggtcatt ttgacccctt cagctcttgt tcaggtgagc
     1861 gtctgtaaca tccatggcta cctgcggtaa aatgggttcc gcggatggtc gaactgcttt
     1921 tgacgctgtt gacggccgga tttccccgct ggaatgtgcg gtaatcagcc gataaattgc
     1981 gcgcccgctt caaaattttt cggaaacgat cgtgtccaaa tcagcctccc gtgcccgtct
     2041 tcacgagatc atccgcaagc gctcgttcgg gcgcggtgaa atcacgctgg cctcgggccg
     2101 caagagcgat ttctacttca acctcaagcc caccatgctc gatcccgaag gcgccacgct
     2161 gcttgcggaa ctgacctacg aggcactgaa agacgacgag ctcgatttca tcggcggcct
     2221 ggaaatgggc gcggtgccgc tggcgggcgc aatcgcgcag atctcctgga tgaagggaca
     2281 tccgatctcg gctttcttcg tgcgcaagaa accgaaggag catggcgcgc ggcttgcagt
     2341 cgagggcctt gccaagggcg agagcctgca gggcaagcgc gtggtggtcg tcgaggacgt
     2401 caccacgacg ggcggttcgg caatgaaggc ggtcgaagcg gtgcgcgagg cgggcggcaa
     2461 ggttgcgctg gtcttgacca tggtcgatcg ggaggaaggc gccaccgata catttgcacg
     2521 agcggggctg cccttccgcg cgctctacac ggccaccgag tttctttgag atttggtccg
     2581 cagcattcta tttctttttc gcgaagtgac ggtctgaata ccgctcgttt accatcccct
     2641 gcttaaggtg aatcggaggc taaggcgcgc ctcacgcctt ggccgaagtc ggtcctgttg
     2701 cgtcgcctgg agtaagcgtt gcgtaacgag tttttgcatc ggcgcctgcc acgccgcatg
     2761 gtgcttgtcg cgaccctttt gatcgcgggt cccctcgcct tcatcccgag cccgacgtcg
     2821 gccgaaggcc tgttcgattt tttcttgggc ggcctttcca agcttcaggc ccacctggct
     2881 ccgccgccgc aagcaggttc cctagccgat cctttcggca acaatcaaca aacctcggcg
     2941 cctcagccga tgatggccgg ctccggacct gcctattgcg tgcgaagctg cgatggaaag
     3001 tattttccgt tgatgcgcgc cgcagcctcg ccgatccaga tgtgccaggc gttctgcccg
     3061 gcgagcccga cgaaggtgtt ttttggcgcg agcatcgatg ccgcctattc cgcaagcggc
     3121 gagcgttatg cggacagcgc aaatgccttt gcctaccgca aggcattgcg cgccgattgc
     3181 acctgcaacg gccgcaaccc tgcgggtctt gcgccggtcg acgtcaccct cgacccgtcg
     3241 cttcgcgagg gcgacgtggt tgcaaccagc gacggcctcg ttgcctattc gggagtgcgc
     3301 gtcggcaacg atcagaccgc ggaatttgcc ccggtcgcga gctatccagg cctgaccgcc
     3361 gatctccgcg cccgcctggg cgagatgaag gttgcgccgg cccgcgccga catggtcgcc
     3421 ggagacgcag cgccggccgc gcccgcaaca ccggtgccgc cggctattcc cgtctcgcca
     3481 aaggccaagc gtgcggacct gaattaagcc gccacctaca ccttgttggt gaaccagatg
     3541 gtcggggcgc cggcgacgcc ggctcgctgc atggcttgct tcagctcttc gctgcccgcg
     3601 aaagcgcgcg cagcttcgag cgtggcaaaa tcgtgcgtga ctgtaacgtc gttgggattg
     3661 tcggcggcct gataaacagc ctcggccgtc actcccttcg ccttctggac cggagcaaag
     3721 tcgtcatacg ctttgcgcca ggctgcgtag ttcgaaaccg tatgtctgac aaacattgtc
     3781 gtcatcgaac ttcccctgca acaaccggtt aaagccggat gctagcatgg ggcggtcgcg
     3841 agtggaccaa gcccttatgc cgcaggcctg cgccgctcag cgccccacga tcacgccgat
     3901 cacgttcggt gcggagaggt atttctcctc gatcgctacg cgcgccgcag cgcggttttc
     3961 cggcgtgagc aggccgcgct tttcggcaag gatcatccag cagaagcccc accagtccgt
     4021 cagcatgccc tgatcgtcga tcaggtcgta gccctgcaag gctgcgaaga tgcgcgcatg
     4081 cgcttcatcc agcgtgtcga ggaaggcatg atcctccttc gaaaacagct cgtccgagat
     4141 gtcgtcggtt gtgtagcccc acaccgacat gcagacggca ttgaactcgc ggtcgatctg
     4201 gtcgtcatcg atcgcgcggg tgcgggaggc ccggtgcagg cgcgacagcg tccgcgcaaa
     4261 atcggcgatg cgggtgagag cgaactggtc gaaggcgatg gtggacatat agtcctcgct
     4321 ggggctgaca gaccatagat gttgtatcgg cacgcgccga atcactatga tatatcaaag
     4381 gcttgctaaa gtgttcttaa ttcgttccag tgagcgaact aaaggttagg tgtcgtcctg
     4441 gcgaaagccc ggatccatta ccccaagaaa cagttatgac gcgacactgg agccggtcat
     4501 gaacgacgca cgcagctact acgtcgacat cctcgccagc cgtcaccatg ggacgctcta
     4561 catcggcatc accaacgaca ttcgcacacg catcgagcaa caccgcgccg gacgaggctc
     4621 ggagtacgtg cagaagtatc gggtttttcg cctcgtgcac gtggaggtat ttgcgactcc
     4681 gcaagaagcg atcgcgcggg aaaagcagct aaaattctgg aaacgcgatt ggaagatcaa
     4741 gctgatcgag caagaaaatc cggattggaa tgaccgctcg ggcctcatct ttcaattggg
     4801 gtaatgggtt cctggctttc gccaggacga caggatggaa acagcctgtc ccctcacatc
     4861 gccgacagca tcttgtcgcc gcaatagttg gcgtagaatt ttccgccgca ttgaagcctg
     4921 atcgcggcgc agcgcgcggc cggcgaggcg
//
LOCUS       DATLJT010000349         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1627834,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000349 DATLJT010000000
VERSION     DATLJT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627834"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM910_08130"
     CDS             <1..399
                     /locus_tag="VM910_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVI62542.1"
                     /translation="SRQDPANFSSSAFVTGMSVRAARGHFGMKGPGAIWYRVERPLIE
                     GFGVSQAMRAVVAADFCNGTAAALDFRKWTFINADLTVSMAREPVGAWILLDAESWIG
                     PDGAGLAMARLADQSGYFGRVVQSLVIEER"
     gene            454..942
                     /locus_tag="VM910_08135"
     CDS             454..942
                     /locus_tag="VM910_08135"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI62543.1"
                     /translation="MSADKPSLPLTGGCSCGAIRYEVASFPLLLYTCNCTDCQTASGS
                     AFALNMPVAAKDFRIVKGEPKGWHHVSPSGAEVTSWFCAVCGGRLYGSRKSRPEFMNL
                     RAGTLDDTGWLVPAGHMFVRSAQSWVECAEGAECHEMAPPEFETMAARWRAMWPEFFP
                     EK"
     gene            complement(981..2543)
                     /locus_tag="VM910_08140"
                     /pseudo
     CDS             complement(981..2543)
                     /locus_tag="VM910_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688905.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            2725..>3017
                     /locus_tag="VM910_08145"
     CDS             2725..>3017
                     /locus_tag="VM910_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI62544.1"
                     /translation="MQTTDPLARLRDRLALPLIAAPMFLVSGVELVVAACRSGVIGAF
                     PTVNCRGSEELDAWLGDITARLRQHADAQGRLPAPVCPNLIVHRSNARLDADL"
BASE COUNT          519 a          965 c          965 g          568 t
ORIGIN      
        1 tcgcgtcagg accccgccaa tttttcctcg agcgccttcg tcacgggcat gtcggtgcgc
       61 gcggcgcgcg gccatttcgg catgaaaggt ccgggcgcga tctggtaccg cgtcgagcgg
      121 ccgctgatcg aaggtttcgg cgtgtcgcag gcgatgcggg cggtggttgc cgccgacttc
      181 tgcaacggca ccgcggccgc gctcgatttc cggaaatgga ccttcatcaa tgccgacctc
      241 acggtcagca tggcgcgcga gcccgtcggc gcgtggatcc tgctcgatgc ggaaagctgg
      301 atcggtcccg atggtgcggg gcttgcaatg gcgcgccttg cagatcaaag cggttatttc
      361 ggacgcgtcg tgcagagcct cgtcatcgag gagcgatagc agcttgccgt ctttctcgat
      421 agacttcagc acctgttcga cgcggagatg aaaatgagcg cggacaaacc ttcgctcccc
      481 ctgaccggtg gctgctcctg cggcgcgatc cgttacgagg tcgcgagctt tccgcttctg
      541 ctctacacct gcaattgtac ggactgtcag accgccagcg gcagcgcatt tgctctcaac
      601 atgccggtgg cggcaaagga ctttcgcatc gtcaagggcg aaccgaaagg ctggcaccat
      661 gtctcgccgt cgggagccga ggtgacatcg tggttttgcg cggtctgtgg ggggcggctc
      721 tacggctcgc gcaagagccg ccccgaattc atgaatcttc gcgccggcac gctcgacgac
      781 accggctggc tggtgccggc cggccacatg ttcgtgcgga gcgcgcagag ctgggtggag
      841 tgcgcggaag gtgccgaatg tcacgagatg gcgccgcccg agttcgagac gatggccgca
      901 aggtggcgcg cgatgtggcc ggagttcttc ccggaaaagt agctgccgat cgatttgcga
      961 agggataaag cgggcgcgcg ctatcccacc atccgctcgc ggccggccca gtagcgcgct
     1021 cgcagcacct ttttgtcgac tttgcccacg ccggtcatcg gcaattcctc gacgaactgg
     1081 atctgcttcg gtgcatgcgc cgagcccttg cgcgccttca cgaggttgat caattcgtcg
     1141 gcatcgggat gcgtcccctc gcgcgcaacc accaccgctg tcaccgcctc gccccatttg
     1201 tcgtcgggca cgccgaccac ggcacacatc gcgacatcgg cgtgttgcga caacacgtcc
     1261 tcgacctcgc gcgggaaaat gttgaagccg cccgatacga tcatgtcctt cttgcggtcg
     1321 aggatgaaca tgtaaccgcg ctcgtccttg cgcgcgacgt cgccggtgtg cagccagccg
     1381 ttcttcagcg tctcctcggt cgtctccgga cgcttccagt attcggccat cacgtgcggc
     1441 gcgcgcacgc agatttcgcc ggcctcaccg ctttcgactt cgtcatcgtc gccgtccagg
     1501 atcttaacct cacaggcagc gatcggaaag ccgcaggaca ggaacagctc cggcatcgcg
     1561 ggatcgtggt cggccttgcg tagaaccgag accggatagc attcggtctg gccgtaaagc
     1621 tgcgagaaca ccgggccgat ccgctcgatg ccctcgacca gccgcgacgg cgacattgcg
     1681 gatgcaccgt agagcagcag ttcgagcgag gagaggtcgg tcctggcgag cgcggggtga
     1741 tcgagcagca cgtagatcat ggtcggcacg aacagggtga aattgatgcg ctcctgttcg
     1801 atggttttca gcaccgcttc gggatcgaaa cccttgagca tgtgcaccgt gccgccgcgc
     1861 atcagggtcg gcagcacctt ggtgccggcg acatggctga tcggcgcgac cgtgagatag
     1921 cgtgcggcct ccggaatttc gaaatcggcg agaatggcgc tggcgaaacc accatattca
     1981 cgatgatagc gcagcgcgcc cttggacttg cccgtggtgc cgccggtgta attcagcgtc
     2041 gcgaaatcat cggcacgcgc aaagcagcgc gcggtcgcat gacccgcctc ctcgattgtg
     2101 gcgaggaggt caatgccgta atttgcgggt ccgagtgtga acacggtcat gacgccctgc
     2161 acttttgcgg cgagttcgcc gccccggtcg ccgaaggcgt tcgcgtcgat caccagcatc
     2221 tcggcttcgg aatcttcgag ctgaaacaac tgatcatcga gcgatccgag cggatgcagc
     2281 caggtgagcg ccagccgcga gagctgcgcc gctacgcccg cgcatcaggt gtcggcacgg
     2341 ttggcggtga ggaaggccac gcgcgccccc ggcggcaaac caagcttcat gaacacggcc
     2401 tgcatccgcc cgatcaggtc cgtcgcgccg cgataggtca gcgatccgcc gggccaactg
     2461 aacgccgtgc gcgacggata gcgcgcgagc gcccgcaagg tctgcccgca gacggtcgga
     2521 aatgtataga gcggattact catttgaccc tcccggcttt ggtctgccgt tattgccgtc
     2581 atcccggaat gcgcgaggcg cagacctgga aattggagcg ctgcttcgca tcgccccggt
     2641 atgacgttga gcgattccga cctctttgtg ttgggcgttc gatcgtgcca atgtcgggtc
     2701 tcacgctagc aaggacgaag gcctttgcaa acaaccgatc ccctcgcacg cctccgcgac
     2761 cgcctcgcgc tgccgctgat cgccgcgccg atgtttctgg tctctggcgt cgaactggtg
     2821 gttgccgcct gccgatctgg ggtgatcggt gcgtttccga ccgtaaattg ccgcggctcc
     2881 gaagaactcg acgcctggct cggggacatc acagccaggc tgaggcagca tgccgacgcg
     2941 cagggcaggt tgcctgcgcc ggtctgtccg aacctgatcg tgcaccgctc caatgcacgg
     3001 cttgacgccg atctcaa
//
LOCUS       DATLJT010000350         1698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3236626,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000350 DATLJT010000000
VERSION     DATLJT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236626"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..740
                     /locus_tag="VM910_08150"
     CDS             240..740
                     /locus_tag="VM910_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62545.1"
                     /translation="MTGHIGRREFITLLGGAAAARSLAARSQQAGKIWRLGLLQPGAP
                     PEPLVDAIRARLRELGYVEGRDLTVEARWAEGKLERLDALAAELVSLNVDVIVAFTTP
                     AALAARHATNTIPIVFTAVGDPVGAGVVASLARPGGNATGFSGLATEMSGKRLDILRK
                     IVVAML"
     gene            754..1209
                     /locus_tag="VM910_08155"
     CDS             754..1209
                     /locus_tag="VM910_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62546.1"
                     /translation="MVLRANQAQDAAAKLGVSIQSVGVHDLVDFEPAFAAIASGGANA
                     MLTLADGVTVQHRKRIVDFALRQRLPAIYETREFVQYGGLVSYGSDILDTQRRAAGYV
                     DQIFKGTKPADLPVQQPTKFVLVLNLKTAKALGIELPPSLSAIADEVIE"
     gene            1468..>1698
                     /locus_tag="VM910_08160"
     CDS             1468..>1698
                     /locus_tag="VM910_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62547.1"
                     /translation="MTVTIGRRELLAALGGAAVAWPLAARAQQPRKLQTIGFMGQSTR
                     SAASEWVAPFVQRLHELGWIEGRTVAIEYRWAE"
BASE COUNT          333 a          502 c          542 g          321 t
ORIGIN      
        1 gcggatgaaa tcaccgaata tcttgcacgg catcagtacg atttatcacc gacgttgcgg
       61 attgagcttg agcggcgtca cgtctgccta tagcgcagac ggacctcttc gacgacgcgc
      121 gagatcggcc gaatgtcgaa gaccaagcga aggtatcgtg ccccgcgcgt aactagagcg
      181 aagtgatcga atagcgatgt aatattcttt gactgggcat gcctccgagg gaggggagca
      241 tgacaggtca cataggaagg cgcgagttca tcacgctgct tggcggcgcg gcggcggcgc
      301 ggtcgcttgc ggcgcgctcg cagcaggcag gtaagatctg gcgtctgggc ttgcttcagc
      361 ccggcgcgcc acctgagcca ctcgtcgacg cgatccgcgc gaggctgcgg gaacttggtt
      421 acgtggaagg ccgtgacctc actgttgagg cccgatgggc tgaaggcaag ctcgaacgac
      481 ttgacgcgct tgccgccgaa ttggttagcc tgaatgtgga tgtgattgtc gcattcacga
      541 cgcctgccgc gctcgccgcg cgacatgcga ccaacaccat cccaatcgtt ttcaccgccg
      601 tcggtgatcc ggtgggagcc ggcgtcgtgg ccagcctcgc ccgtcctggc ggaaacgcga
      661 ccggtttttc ggggctggcg acagaaatga gcggaaagcg gctcgatatt ctccggaaga
      721 tcgttgtagc catgctgtga taccaatccg ggcatggtgc tccgcgccaa tcaagcacag
      781 gatgccgccg caaaactcgg cgtgagcatc caatccgtcg gcgtgcacga tctggtcgac
      841 ttcgagcctg cctttgccgc gatcgcgagc ggcggagcca acgcgatgtt gacgctggca
      901 gatggggtca cggttcaaca ccggaagcga attgtggatt ttgcgttacg gcagcgcctg
      961 ccggcaatct acgaaacacg agagtttgtg caatacggcg gtctcgtttc ttacgggagc
     1021 gacatcctcg acactcagcg gcgtgccgcc ggctacgttg atcaaatatt caagggcacc
     1081 aagcctgctg atctgccggt ccaacaaccg acaaagtttg ttctcgtcct caatctgaag
     1141 actgccaaag ctctcggcat cgagctgccg cccagcttgt cagctatcgc cgacgaggtg
     1201 atcgagtgaa gaggcgcagc gcgcatcata gacttgagtc gtgatgttag ttctcaaacg
     1261 cgcctccgcg agccgcccct ccggcgaatg gaatgacgac gacttcgacg tgcgcgccga
     1321 cggcgctgtt gtcggccgca tcaccgcttc cgaatttgaa gcccacgaca gaggtggaag
     1381 caaccagcat cccaccccct gaatgtgaag actgcaccat cgtggcgtgg tatcgtttgt
     1441 tttacgcacc tcgcacggga ggggcgtatg acagtcacca tcggacggcg ggaattactg
     1501 gccgcactcg gcggcgcggc ggtggcatgg ccgcttgcgg cgcgggcgca gcagccgagg
     1561 aaactgcaga ccatcgggtt catgggtcag agcacgcgtt cggccgccag cgaatgggtc
     1621 gcccctttcg tgcagcgact gcatgagctc ggttggatcg agggccgcac cgtcgcgatc
     1681 gagtaccgct gggccgag
//
LOCUS       DATLJT010000351         2209 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2777251,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000351 DATLJT010000000
VERSION     DATLJT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2777251"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..202
                     /locus_tag="VM910_08165"
                     /pseudo
     CDS             1..202
                     /locus_tag="VM910_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494081.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            367..1662
                     /locus_tag="VM910_08170"
     CDS             367..1662
                     /locus_tag="VM910_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HVI62548.1"
                     /translation="MGRLQDLLSGLRETCAGVPDKRRGKNSHYAMADIGMAAFSVFFM
                     QSPSFLAHQRQLAQGHGHGRSNCETLFGMTRIPSDNHVRDMLDPVPPECFHARFPHAV
                     EVLEAGGGLAAFRRLGHHVLIAFDGTEYFRSAKLHCPRCSTRKRSGGTTEYFHTLMAA
                     TLVAPGHNRVVPLQPEFVVPQDGHDKQDCESRAARRWLAAHGASYARLDPVYLGDDLY
                     SRQPICQAVGAAGGHFLFVCKPSSHPTIEEYLTGVELPELTERVKHGRQRFTHTYRWL
                     GDVPLRDGADAMTVNWLMIEIRNPAGAVTYRNSFITDLPVGRDTVVELAACGRARWKI
                     ENESFNTLKTKGYNLEHNFGHGQQHLSAVLAILNLLAFAFHTVADLTYDLWLQAVNKA
                     GARSRFFEHLRAITIFLVFPSWQSLLTTLAFARPPSQPP"
     gene            1770..>2209
                     /locus_tag="VM910_08175"
     CDS             1770..>2209
                     /locus_tag="VM910_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62549.1"
                     /translation="MHRVTSVSSYAVATACLLLAAGGPLSQAQGQPAASPAASAGLDY
                     EFFKTKVQPVFLNKREGHTRCVVCHTINNAPFHLVRLSPGSTTWNEEQSRQNFQLVQK
                     VAVPGYYEDSKLVIHPLAEQAGGDPHHGGGQQFESKNDPDWQTL"
BASE COUNT          403 a          700 c          684 g          422 t
ORIGIN      
        1 ccaggatggc gcgcgacaag gggctgcttg caaagcttgc cgccggcgat tggattgccc
       61 ggcgcgagaa cctgatcatc gtcggcaaga ccggcctcgg caaaagttgg ctcgcctgcg
      121 cgctcgccac aaggcctgcc gcgacgaccg ctcggtgctc taccaccgca tcccccgcct
      181 gttcgatgcg ctggcgctcg catgatcgcc ccgaataccg ctgacgagcg gctcaccaca
      241 agccatcgtg ccaaattgca gcagttctca ttttgacgag gaaccggggt agagggggtg
      301 tgacagattg aatcggggtt cgctcgggct gttttcaggt gggcgcgagt cggattcaag
      361 ctccgtgtgg gcaggctgca ggatcttcta tcagggctgc gggagacctg tgcgggtgtg
      421 ccggacaagc ggcgtgggaa aaacagccat tacgcgatgg ccgatatcgg catggcggct
      481 ttttcggtgt ttttcatgca aagcccgtcg ttcctggccc atcagcggca gttggcgcag
      541 gggcacggcc atggtcgctc gaattgcgag acgctgttcg ggatgacgcg gattccgagc
      601 gacaaccatg ttcgcgacat gttggacccg gtgccgccgg agtgctttca tgcacggttt
      661 ccccatgcgg tggaggtgtt ggaggctggc ggcgggctcg cggccttccg tcggctgggc
      721 catcacgtgc tgatcgcgtt cgacggcacg gaatattttc gctccgccaa gctgcattgt
      781 ccgcgctgtt cgacgcgcaa gcgtagcggc ggcacgaccg aatatttcca taccctgatg
      841 gcggcgaccc tggtggcgcc aggccacaac cgggtggtgc cgctgcagcc ggagttcgtg
      901 gtgccgcagg atgggcacga caagcaggac tgtgagagca gggcggcacg acgctggctc
      961 gccgcccatg gcgcgagcta tgcaaggctc gatcctgtct atctcggcga tgacctctac
     1021 tcccgccagc cgatctgcca ggcggtgggg gcggcgggcg ggcatttcct gttcgtctgc
     1081 aaaccgtcgt cccatccgac catcgaagaa tacttgaccg gggtcgaact tcccgagctg
     1141 acggagcgcg tcaagcatgg ccgccagcgc ttcacccaca cctatcgctg gcttggcgat
     1201 gtgccgctgc gcgacggcgc cgatgccatg acggtcaact ggctgatgat cgagattcgc
     1261 aaccccgccg gcgcggtgac ctatcgcaac agcttcatca ccgatctgcc cgtcggccgc
     1321 gacaccgtcg tcgaattggc cgcctgcggc cgggcgcgct ggaagatcga gaacgaaagc
     1381 ttcaacaccc tgaaaaccaa gggctacaac ctcgaacaca atttcggcca cggccagcag
     1441 cacctctctg ccgtccttgc catcctcaac ctgctggcct tcgcgttcca caccgtcgcc
     1501 gacctgacct acgatctctg gcttcaagcg gtcaataagg ccggcgccag aagccggttc
     1561 ttcgaacatc tgcgagcgat caccatcttc ctcgtttttc cctcctggca gagcctgctg
     1621 actaccctgg cattcgcccg gcccccgtca cagccgccat gacaggttct caaaatgaga
     1681 gctgcacgct ttaactgcaa cttgcaagcg cttttgcccg ggcataggat gcatgtttgc
     1741 taaccacaat actttgttgg gaggttatca tgcaccgagt gacgagcgtg tcgtcttatg
     1801 cggtcgcgac tgcgtgcctg ttgttggcgg cgggcgggcc gttgagccag gcgcaaggcc
     1861 agccggccgc ttccccggct gcatcggccg ggttggatta cgagttcttc aagaccaagg
     1921 tacaacccgt cttcctgaac aagcgtgaag gccacacgcg atgtgtcgtg tgccacacga
     1981 tcaacaacgc gccgttccac ctggtgcggc tctcgcccgg gagcacgacg tggaatgaag
     2041 aacagtcgcg ccagaacttc cagttggtcc agaaggttgc cgtgcccggc tactacgagg
     2101 acagcaagct cgtcatccat ccgctcgccg aacaggccgg cggtgacccc catcatggtg
     2161 gcggccagca attcgaatcg aagaacgacc cggactggca gacgttgaa
//
LOCUS       DATLJT010000352         6757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_479563,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000352 DATLJT010000000
VERSION     DATLJT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479563"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..536
                     /locus_tag="VM910_08180"
     CDS             66..536
                     /locus_tag="VM910_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVI62550.1"
                     /translation="MRRCLLVLLAALSVLILPCAGGAERLAAAELQPLEIASKNGVHV
                     FAVEMASTPEEQAKGLMFRRELPEGQGMLFDFHTEQPTSFWMKNTYIPLDMIFIRGDG
                     RILRIAENTVPLSETLVPSGGPVRAVLEVIGGTARKLGIAPGDRVGHAIFGGGR"
     gene            606..682
                     /locus_tag="VM910_08185"
     tRNA            606..682
                     /locus_tag="VM910_08185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:640..642,aa:Pro,seq:tgg)
     gene            complement(747..1958)
                     /locus_tag="VM910_08190"
     CDS             complement(747..1958)
                     /locus_tag="VM910_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI62551.1"
                     /translation="MPPRSNHPGLKWRRTTSGRQPYWVAKQVVRDPKGFPERTIRLPA
                     GADEDTLGELCRDYTARLLAWLAAPEGEPTVAYDGTIRSLSRLYQQHADSPFHDVKRN
                     TRKTYSDSLKVIESTVGNRLVRVVTIVDVKRWFKLWRAPKVEGGDPRGSRAHDAVSML
                     RTILRFGFALGYPDCTALVERLKMVRFENAGAREQEMTAAQTMAFIRVAIGRGDDRGR
                     NMAIGVAAQFELALRQKDIIGEWGPARPEVAGAVYAGAEMWTGAFRWENIPGWRLRLK
                     TSKNRARAEFCLDDYPMLFPLLEVVPHAERSGAIVKGEYGLPIRERTYRKWFREIARA
                     AGIPDEVWSMDSRAGAATEADAAGADLKSISDLLTHAEPRTTFRYIRSTNKRMREVAK
                     ARARAREANDP"
     gene            complement(2373..2834)
                     /locus_tag="VM910_08195"
     CDS             complement(2373..2834)
                     /locus_tag="VM910_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI62552.1"
                     /translation="MTKVKVRHYVVKGGKAFWQPTPKMKALGFHAVPLGPDGPDAWAI
                     AERWNGRWDKTRNCEVPSPAWPQPRTCRRERVEEASESLAAFMGPDAEPFEIRLLGDQ
                     WTISAVKTGDDLDTLQALRKQGMSVRDIAERTGLSKSTVQRKLKIGDDDDE"
     gene            complement(3093..3641)
                     /locus_tag="VM910_08200"
     CDS             complement(3093..3641)
                     /locus_tag="VM910_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62553.1"
                     /translation="MSALIQAFDVPKGDVPAFHGRIIALQKAGLLGAKNMPGKGRALA
                     YAPDLMHRLVFACELLQLGLAPKVIVNTVADLWDRKIRAIFTLAAKAAAAAPSGDDVV
                     MYLGGVKLMTDSWADAVPNVNRCALKDLRWHVAQWMGMREEDAAPGNLPPRVIMTNLT
                     HRLRRFHTALADAEHKKGAPNV"
     gene            3836..5662
                     /locus_tag="VM910_08205"
     CDS             3836..5662
                     /locus_tag="VM910_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI62554.1"
                     /translation="MKRARISKRPYTYKGKTEDRWLVQWADLKGARREKWFHNKRQAE
                     EFATRIDRELADNIHVAERGTITFGKLCEAWLRHEERRADPRNRNADLRKGSLAAKQS
                     VAKNHLLPAFGNMKLNKITSADIKRFIEEQAYRNSHWTARRHKSFVSQVLKFAVENDW
                     LRVSPLTNKPIKVPGEEPIRKDIPEVEDVARLLNVLLNRPRPKRFDLNQWSSVRVQIV
                     LGALLGLRPGEIAGLQWENVDLEKWIIRIEHSLSFYDGLKTPKTRSSYRILDLEPVSH
                     RVLTEHAEQTRSGSTRPNSGRALTGYVLTTKQRGFVMPTTLRWRHNNILRLAGLIGPN
                     GKSNFSPHALRHFAGSLWLAEGMALKDVSWRLGHQTTRMTEKVYLHQLRHDKRAKQVM
                     DRVAAARFPGIRGTPNSMPLKIADASNVIDLEAETIASLPLPEPPAAPTKPAPPIPPI
                     PRLQPDRYTARAPGRRPRTLEDAPQWLDEALDLVEQGHTIVEIARRLQRGERTVTEWL
                     KVAGLAKPGVHAAAVRTAKLEARFAEMSAQGYQVVEIARRLGCSLNTVKRWRIGRTPG
                     NRLRQENGVAEKLANQLKTSVKSARERKPNTTNFRGKQLNLL"
     gene            5622..6392
                     /locus_tag="VM910_08210"
                     /pseudo
     CDS             5622..6392
                     /locus_tag="VM910_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611761.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1309 a         2050 c         2099 g         1299 t
ORIGIN      
        1 tgccccgatc tgcaagatct caagtctctc tgctatcatc ggcgcgcgtc aatcgaggct
       61 ttgtcatgcg ccgttgcctg cttgttctcc tcgccgcgct gtcggtcctg atcctgccgt
      121 gcgcgggcgg cgccgaacgc ctcgcggcgg cggaattgca gccgctcgag atcgccagca
      181 agaacggcgt gcacgtcttc gcggtcgaga tggcctcgac gccggaggag caggcgaaag
      241 ggctcatgtt tcgccgcgaa ctcccggaag ggcagggcat gctgttcgac ttccacacgg
      301 aacagccgac cagcttctgg atgaagaaca cttacatccc gctcgacatg atcttcatcc
      361 gcggcgacgg gcgcatcctg cgcatcgccg agaacacggt gccgctctcg gagacactcg
      421 tgccctccgg cgggccggtg cgcgcggtgc tggaagtgat cggcggcacc gccaggaagc
      481 ttggcatcgc gcccggagac cgcgtggggc acgcgatctt cggtggcggc cgatagctcc
      541 gttgcggcgg cccggcgcgg cttgctggcg caaaccgaac cgtgtatcga ggcccggcac
      601 ggcgccgggg catagcgcag cctggtagcg cgggagtttt gggtactcca ggtcgcaggt
      661 tcaaatcctg ctgccccgac cagctaattc tttgattcgt cagagtcccg ttctgacgcc
      721 gttctgacgc tccgttctga cgacgttcac ggatcattag cctcgcgcgc tcgcgctctg
      781 gcctttgcca cctcgcgcat tcgcttattg gtgctgcgga tatatcggaa tgtggtacgc
      841 ggttctgcat gtgtcagcag gtcggaaata gacttgagat ccgcgccggc tgcatcggcc
      901 tcggtcgctg cgccggcgcg cgaatccatg gaccaaacct cgtccgggat cccggcagca
      961 cgggcgatct cccggaacca tttgcggtag gtgcgctcac ggatgggcag gccgtactcg
     1021 cccttcacga tcgcaccgct acgctccgcg tgcggcacta cctcaagcag cgggaacagc
     1081 atcgggtagt catccaagca aaattctgcc cgggcccgat tcttcgaggt cttaagccgg
     1141 agccgccagc ccgggatgtt ctcccaacgg aatgcacccg tccacatttc ggcaccagca
     1201 tagaccgcgc cagcgacctc gggacgagcc gggccccact cgccgatgat gtccttttgt
     1261 cgaagtgcca gctcaaattg agccgccact ccaatcgcca tattgcgtcc gcgatcgtcg
     1321 ccgcggccaa tcgccacgcg aatgaaggcc attgtctgag ccgccgtcat ctcctgttcg
     1381 cgagcgccgg cattctcgaa gcgaaccatc ttcagtcgct cgacaagggc cgtgcagtcg
     1441 ggataaccga gcgcgaagcc gaaccgcaga atggtgcgaa gcatagacac ggcgtcatgc
     1501 gcacggctgc cacggggatc gccgccctca accttcggtg cgcgccacaa cttgaaccac
     1561 cgcttgacgt cgacgatggt gaccacacgc accaaccgat tccccacggt gctttcgatt
     1621 accttgaggc tgtccgaata agtcttccgg gtattgcgct taacgtcgtg aaagggtgaa
     1681 tcagcgtgct gctggtagag ccgcgacaga ctgcggatgg tgccgtcata agccaccgta
     1741 ggctcgccct cgggagcagc gagccaggca agtagccgcg cggtgtaatc gcggcaaagc
     1801 tcgccgagag tgtcctcatc ggcgcccgcc ggcagccgga tggtgcgctc cgggaagcct
     1861 ttggggtcgc gcacgacttg cttggcaacc caataaggtt ggcgtccgct ggtcgtccgt
     1921 cgccatttca ggcccgggtg attgctgcgc gggggcatca aagctctctg gcccgtcctg
     1981 gccgggctcg atttggccat tctcacgaac tccgttttcg tcgtctagcc atgccttgac
     2041 cgccggccag tagcggccgc ggaacagagc atgaattttg gggaaatccg ggtacttcgc
     2101 ttcgagcgcg cgcagtgcgg agcgaaagcg atcccgaccg atctttggat tgatgcgtcg
     2161 gcgaagctct tcgtcgtcga cgaagagatg ccggtcatca tcgatagctt cggagcgcat
     2221 ggcagctacc gggaaatcct cttatcggcg gtgatggttc tcaagcctgc gtccaactgt
     2281 cccgactgtc ccgactgtcc caattgtccc aactatcccc ggccgtctct ccttagcgaa
     2341 tgtcccgagg gacagccgac ccggtcgcag ggtcattcgt catcatcatc cccgatcttc
     2401 aacttgcgtt ggacggtcga tttggagagg ccagtgcgct cggcaatgtc gcggaccgac
     2461 attccctgct ttcgaagcgc ttgaagcgta tcgagatcgt cacccgtttt gaccgcgctg
     2521 atggtccact gatcgccgag taacctgatt tcgaatggct cggcgtcggg ccccatgaaa
     2581 gccgcgagac tttccgaagc ctcttcgacg cgctcccggc gacaggttct cggctgcggc
     2641 catgcagggg agggcacttc gcagttcctg gtcttatccc agcgcccgtt ccagcgctcg
     2701 gcgattgccc atgcatcggg gccgtccggc cccaacggca cagcgtggaa tccgagcgcc
     2761 ttcattttcg gcgtgggctg ccagaacgcc ttgccgccct tgaccacata gtgccggact
     2821 ttgaccttgg tcattctcgc ctcttggacg ccacaacgct tgcgtcgcgc ggtgcaatcg
     2881 cttcgctcag ctcgggctcg acgggaccga aagagagcga aaccgatccg tccggcgcca
     2941 gcgttgcgcc ggcgacgccg agcccagcct tcttggctgc agcgatggca ttgtcgaggc
     3001 gcggcggctt gcgctggcgc ttcggcttaa gcgcttcgcc gagcgtccaa ttgttggcgc
     3061 gcagctcgtg aacaaacggc ttcttgactc gctcatacgt taggcgcccc cttcttgtgc
     3121 tcggcgtcgg cgagcgcggt atggaagcgc cgcagccggt gggtgaggtt ggtcatgatg
     3181 acgcgcggcg gcagattgcc cggcgccgcg tcttcttctc gcatgcccat ccattgggcg
     3241 acatgccagc gcagatcctt gagcgcgcag cggttcacat tgggcaccgc gtctgcccag
     3301 gagtcggtca tgagcttgac gccgcctagg tacatcacga cgtcgtcgcc gctgggcgcc
     3361 gccgcagccg ccttcgccgc cagcgtgaag atcgcgcgga ttttgcggtc ccacaggtcc
     3421 gccacggtgt tgacgatgac cttgggcgca aggcccagct gcagaagctc gcaggcaaac
     3481 acgaggcggt gcatgaggtc gggggcgtag gccagcgcgc ggcccttgcc gggcatattc
     3541 ttggccccga gcaggccggc cttctgtaga gcgatgattc ggccgtggaa ggccggcacg
     3601 tcgcccttgg gcacgtcgaa cgcctggatc agcgcgctca aaacgctacc gaagcttgcc
     3661 gtaaacatcc tgggcctttg ccgggcttta ccgagctttc gctatttgcc agaaaaggct
     3721 cctaaaagca atggtaatgg ctgtgttgcc atttagctgg cggtcgacta cacttatggc
     3781 ggttttacca tttcaggagg acgtgagaca gggcgacgta aggggaggga atgccgtgaa
     3841 gcgtgccaga atttcaaagc ggccctacac ctacaagggc aagaccgagg atcgttggct
     3901 ggtccagtgg gccgacctaa agggagcccg gcgcgagaaa tggtttcaca acaagcgcca
     3961 ggccgaggaa tttgccacca ggatcgatcg agagcttgcg gacaacatac acgtcgcaga
     4021 acgcggaacg atcactttcg gcaagctttg tgaggcgtgg ctcaggcatg aagagcgaag
     4081 agccgacccc aggaaccgaa atgcagactt gagaaaaggt agcttggctg caaagcaaag
     4141 cgtcgccaaa aaccatctgt tgcctgcctt cggcaacatg aaactgaaca agatcacttc
     4201 ggcggacata aagcgattta tcgaagagca ggcttatcga aattcccact ggacggcgag
     4261 acggcataag agcttcgtca gccaagtcct caagtttgcg gtggagaatg attggctgag
     4321 agtgagcccg ctaacgaata aacccatcaa ggttcctggc gaggaaccca ttcggaagga
     4381 tattcctgag gtggaagatg ttgcgcgact cctcaatgtc ctactcaatc ggccgcgacc
     4441 aaagcgattc gacttgaatc agtggtcaag cgtgcgagtg caaatcgtgc ttggcgcgct
     4501 gttgggcttg cgccctggag aaatcgctgg cctgcaatgg gagaacgtcg atctggagaa
     4561 atggataatt cggatcgagc acagcctttc gttctatgac ggtctgaaga cccccaaaac
     4621 gcgatcaagt tatcggatat tggatctaga gccggtatcc catcgcgtct tgaccgaaca
     4681 cgctgaacag acgcggagcg gttcaactcg tccgaactcg ggtcgggccc tgactggcta
     4741 tgttttgacg actaagcagc gcgggttcgt gatgccaacg actctccgct ggcgccataa
     4801 caatatccta cggctcgccg gactgatcgg gcctaacggc aaatcgaatt tttcaccaca
     4861 tgcgctcagg cattttgcgg gaagtctttg gctcgcggaa ggtatggcgc tcaaggacgt
     4921 ttcgtggcgt ctcggtcacc agaccacgag aatgactgaa aaggtgtacc tgcatcaatt
     4981 gcgccacgac aagcgcgcca agcaggtaat ggaccgcgta gcagccgcgc gatttccggg
     5041 tattcgtggg acgccgaact cgatgccttt gaaaattgca gacgcttcca atgtcatcga
     5101 tctcgaagcg gagactattg cgtcgctgcc gctgccggag ccgccggctg ctcccacaaa
     5161 acccgcgccg ccgattccgc caatcccgcg gttgcagccg gatcgataca ccgcgcgggc
     5221 gccaggtcgt cggcctcgga ccttggaaga tgcaccgcaa tggctcgatg aggcgctcga
     5281 tttggtcgag caaggtcaca ccattgtgga aattgctcgc cgtctgcagc ggggtgagag
     5341 aacggtaacg gaatggctga aggtggccgg gctggccaag ccgggagttc atgcagccgc
     5401 cgtgcggaca gccaagctgg aagccaggtt cgcggagatg tccgcgcaag gttatcaggt
     5461 agttgaaatc gcgcgtcggc tcggatgttc gctgaacacg gtcaagcgct ggcggatcgg
     5521 tcggactccg ggaaacaggc ttcggcagga aaatggggtg gccgaaaaat tagctaacca
     5581 attgaaaaca agcgtaaaat cggcgcgaga gcgcaagcca aatacgacaa atttccgggg
     5641 aaaacagctt aacctgttgt aatgctatct tcacttggtc aagtgcgggg tccgcgccgc
     5701 tttacgaggc tatcgaaacg tcgactggcg cgcaggccga tccacagcaa gatcgcggtg
     5761 gacttccgct tcgccaggag tttggcggcc tgaagaccga gcaggtgaag cgcctgaagg
     5821 accttgagct tgaaaacagc cgaccgcgca aggcggtctc tgatttgacg ctggacaagc
     5881 tcattctgca ggaggcttcc cggggaaact tctaagcccc gcgcgtcgcc gtgcctgcat
     5941 tgagcaggtg cgagcgcaac tgcatgtctc cgagcgtcgc gcctgtgcgg cgctcggaca
     6001 gcatcgctct acgcaacgca aggtcccgcg cggccgtgag gacgaggagc gtctgacggc
     6061 cgatattatc gagcttagca atacggccgc tacggctatc gcaaggtcgc cgagctgctg
     6121 cgccaggcgg gctggataat caacgacaag cgggtcgagc gtgtctggcg acgcgagggg
     6181 ctgaaggtcc caccgaagca acccaagcgc ggcaggctct ggctggcgga cggatcgtgc
     6241 atccgcctgc ggccggagcg gcgcaatcat gtctggccct agtgatcgac gttctgtccg
     6301 acctcttcat cctgcggggc attcccgctc acatccgctc cgacaacgga ccggagttcg
     6361 tcgccaaggc ggtgcaggaa tggatcgcgg cgataatggt ggttcgagtt gcggttttaa
     6421 ttgtaaataa caggccggaa aacaatggag cggatccggc gacacgtgaa ggttggttct
     6481 tcacacgacg cgcgatagcg ccggacgcgt tcggagcctt attcactttt caaacagcca
     6541 ctctcgttcc cgcggcgcgt tttcttgcgc ccggggtttt gcaacctttg cttcgctcac
     6601 ccgaatcgag gggtgggcgg agcgccgaga aacgttcggg agctgcgcta agcaccgttg
     6661 ggcgtgccat agcgcgtgaa agacgcgcgt gaacgcgctt tcgccgcggc acgcccggcg
     6721 cctagcgagg cgccttgcgt cccatgacgc ggcgaac
//
LOCUS       DATLJT010000353         5382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3236932,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000353 DATLJT010000000
VERSION     DATLJT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3236932"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /gene="phaC"
                     /locus_tag="VM910_08215"
     CDS             complement(<1..1623)
                     /gene="phaC"
                     /locus_tag="VM910_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HVI62555.1"
                     /translation="MDSPSLQSGPAAYLESLMRAGQQATKQFDDALAAAMGVEGKPAK
                     GEDPSPFAVAANLQQQYWSPVMDFWRGFLAGTPAGANPDNRPSRDRRFKDEAWQNSPF
                     YDLMKHSYLATAKQMADFVDQAQVDDKSKLQLRFYARQFIDAMSPSNFPATNPDVIRT
                     AIQTRATSLVTGMQNLIEDLQKGRITRVDESAFEVGRNLASTPGSVIFENELIQLIQY
                     TPTTAEVEKTPLVIVPPCINKYYLLDLGAGNSFVEYAVAQGHKVFLISWRSAVSETQY
                     LTWDDYLNLGPLKAIDVARDIAGVEKVHALGFCVGGTILGCAAGVLAARGENRIATMT
                     LLTTMIDFSDTGEIGLLIDDAAVALREATIGSGGILPGKELAFTFGTLRANDLIWRYV
                     VDSYLKGAPPEAFDLLYWDSDSVSLPGPMYCWYTRNTYVENKLKEANATTQCGLRIDL
                     SKVKVPLYVLASREDHIVPWQSAYRSKDILGKEARFVLAASGHVAGVINPPARKKRSH
                     WINDDLNCDATEWLEKAREEPGSWWPDWDAWMK"
     gene            1941..2429
                     /locus_tag="VM910_08220"
     CDS             1941..2429
                     /locus_tag="VM910_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI62556.1"
                     /translation="MMDHPLRRPEEILSYRSLRQILAAKPKALWIVGPRESTLSAMHL
                     MAEKNIGLVVVVENRAIVGVLSERDCVRRLVLAGKSPEATPVSALMVRDVVKVDIAGT
                     FADCLKLMHEHRIRHLPVVENGALIGVISIRDLLSEAVAHHEKVIGELERERLTMLTS
                     TA"
     gene            2472..3857
                     /locus_tag="VM910_08225"
     CDS             2472..3857
                     /locus_tag="VM910_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HVI62557.1"
                     /translation="MSLLIVLAALFFLMFVAYRGYSVILFAPVAALGAVLLTDPALVA
                     PMFSGVFMEKMVGFIKNYFPVFMLGAVFGKVIELSGFSKSIVSAVIGVLGKGRAVLSI
                     VVVCAILTYGGVSLFVVVFAVYPFAAEMFRQGDIPKRLIPCTIALGAFSFTMDALPGT
                     PQIQNIIPTTFFNTTTWAAPILGLIGSAFVFVVGLAYIENRRRAAHNHGEGYGTNLVN
                     EPEPFEEKELPNPWIALLPLIVVGVANFVLTFAIPRAYGAKYEAMVGDHPIVTQIGPV
                     AAIWAVEGALILGILTVFAFAWRPVLAKFADGSKAAVAGALLASLNTASEYGFGGVIA
                     LLPGFKVVSSALSAIPNPLVNEAVTVTVLAGITGSASGGMSIALAAMSDTFIAAAHAA
                     HIPLEVFHRIAAMASGGMDTLPHNGAVITLLAVTGLTHRQAYKDIFVITLTKTAAVFV
                     AIAWFYLTGIY"
     gene            3938..>5382
                     /locus_tag="VM910_08230"
     CDS             3938..>5382
                     /locus_tag="VM910_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HVI62558.1"
                     /translation="MAVGARRLTLAPVKALDSGKIVTAEEAASLIRDGDTIATGGFVG
                     IGFAEEIAVAIEKLFLAKGEAGPTGSKPGNLTLVYAAGQGDGKERGLNHLGHAGLVRR
                     VIGGHWGLVPKLQQLAIANQIEAYNLPQGVISHLFRDIAAKRPGHLTRVGLGTFVDPR
                     HGGGKINERTKEDLVRVIDVEGEEFLFYRAFPIDIGIIRGTTADPDGNVTMEKEALTL
                     EALAIAMAVRNSDGLVIAQVERIADRGSLNPRQVKIPGIMVDCVVVAKPEHHWQTFAV
                     QYDPAFSSEIRARVDMIPAMEMNERKIIARRAALELSVNSVVNLGIGMPEGIANVTNE
                     EKISDLVTLTAEPGVIGGVPAGGLNFGAAVNAQTVIDQPYQFDFYDGGGLDAAFLGLA
                     QADREGNLNVSKFGPRLAGAGGFINISQNAKKLVFVGTFTAGRMRLAVENGSLRIDKD
                     GGVVKFVREVEHRTFSGAYAIRRGQPVLYVT"
BASE COUNT          991 a         1606 c         1672 g         1113 t
ORIGIN      
        1 tttcatccag gcgtcccaat ccggccacca gcttcccggc tcttcccggg ctttttccag
       61 ccactccgtg gcgtcgcaat tcagatcgtc atttatccaa tgactgcgct ttttgcgggc
      121 cggcgggttg atgacaccgg caacgtgacc gctggcagcc agcacgaaac gcgcctcttt
      181 tccgaggatg tccttcgagc ggtacgcgct ctgccaaggc acgatatggt cttcgcgaga
      241 ggccagcaca taaagcggta ctttgacctt cgagagatcg atccgcaggc cgcattgcgt
      301 ggtcgcattg gcctccttga gcttgttctc gacataggta ttgcgcgtat accagcaata
      361 cattggcccc ggcaggctga cgctgtcgga atcccagtaa agcaggtcaa aggcctcggg
      421 cggcgccccc ttcaggtagc tgtcgacgac atagcgccag atcagatcgt tggcgcgcag
      481 cgtcccgaag gtaaaggcca gttccttgcc cggcaggatg ccgccgctgc cgatcgtcgc
      541 ctcgcgcagg gcgactgccg catcgtcgat gaggagaccg atttcgccgg tatcgctgaa
      601 gtcgatcatt gtggtcagca gcgtcatggt ggcaattctg ttttcgccac gtgcggcgag
      661 aaccccggcg gcgcatccca gaatggtacc tccgacgcag aatcccagcg catggacctt
      721 ttcgacgccg gcaatgtcgc gcgcgacgtc gatcgctttg agcggcccca ggttcaggta
      781 gtcgtcccat gtgagatact gggtctccga taccgcgctg cgccaggaga tcaggaacac
      841 cttatggccc tgagcgacgg catattcgac aaatgaattt cccgcaccga gatcaagcag
      901 atagtatttg ttgatgcagg gcgggacgat aacgagcggc gtcttctcga cctcggccgt
      961 cgtcggcgtg tactggatga gctggatcag ttcattctcg aaaatgaccg atccgggggt
     1021 cgatgccaga ttgcggccga cctcgaaagc ggattcgtcg acccgtgtta tgcgaccctt
     1081 ttgcagatcc tcgatcaggt tctgcattcc ggtcacgagg ctcgtcgcac gcgtctggat
     1141 ggcggtgcga ataacgtcgg ggttggtcgc cgggaagttc gacggactca tcgcatcgat
     1201 gaattgtcgt gcgtagaagc gaagctggag cttcgatttg tcgtcgacct gggcctgatc
     1261 gacaaaatcg gccatttgtt ttgcggtcgc gagataggaa tgcttcatca gatcgtagaa
     1321 gggcgagttc tgccacgctt cgtccttgaa acgtcgatcg cgcgacggtc gattgtccgg
     1381 gttcgcgcct gcgggcgtgc cagccaggaa tccccgccag aaatccataa ccggagacca
     1441 atactgctgc tgcagattgg cggcgaccgc aaagggcgag gggtcttcgc ccttggccgg
     1501 tttgccctcg acccccatgg ccgccgccag cgcatcgtcg aattgcttcg ttgcctgctg
     1561 gccggcgcgc atcaggctct caagataggc ggcggggccg ctttgcaatg acgggctatc
     1621 catttgaacc tccctcctgc gaccttttgg tcggttatcg ttggtgccgt tcacgaaccg
     1681 gccggtgtgt tttcacgctc ggcaactccg gtcgctccaa aaaacctttg ttgcagtgca
     1741 tgacaaccaa gcgaactcca gcttgacata gatcaacagc gaccggctgt cgcctcatca
     1801 cattcaacgc caagacgcgc cgtgtttcca gagggcttga gcggaacctc gccaagtgcg
     1861 tgccgcgccc ggccgcgaag cagacggtgt cggtaggata gtcccgtcca aatcggttcg
     1921 cgccacacgg gaagggacct atgatggatc atccactcag acgtccggaa gaaattctct
     1981 cctaccggtc gcttagacag atcctcgccg ccaagccgaa ggcgctttgg atcgtgggtc
     2041 ccagggaaag cacgctttcg gcgatgcatc tcatggccga aaaaaacatc ggactggtcg
     2101 tggtcgtgga gaatcgagca atcgtcggcg tcctctcgga gcgggattgc gtgcggcgcc
     2161 tcgtgcttgc cgggaaatct cccgaagcca caccggtctc cgccctcatg gttcgggatg
     2221 tcgtcaaggt cgatattgcg ggcacgtttg ccgactgttt gaagttgatg cacgagcacc
     2281 gcattcgtca tctgcccgtg gtcgagaacg gcgcgttgat cggggttatc tcgatccgcg
     2341 atctgttgag cgaggcggtg gcgcaccacg aaaaggtcat tggtgaactc gaacgcgaac
     2401 ggctgacgat gctcacatcg acggcttgat cggcgttcgg ctgatcacca gcaacgggag
     2461 gaggtttcgc cgtgagtctt ctgatcgtcc tcgccgctct ctttttcctg atgtttgtgg
     2521 cctatcgggg ctacagcgtg atcctgttcg ctccggtcgc cgcgcttggc gccgtgctgc
     2581 tgaccgatcc ggcgcttgtc gcgccgatgt tctccggcgt cttcatggag aagatggttg
     2641 gtttcataaa aaactatttc ccggtcttca tgctgggcgc ggtcttcggc aaggtcatcg
     2701 agctttcggg tttctccaag tcgatcgttt cggctgtcat cggtgtgttg ggcaaagggc
     2761 gggcggtcct gtccatcgtc gtcgtctgcg cgatcctgac ctatggcggc gtatcgctgt
     2821 tcgtcgtggt gttcgcggtt tatccgttcg ccgccgagat gttccgacag ggtgatatcc
     2881 ccaagcgtct cattccctgc acgatcgccc ttggggcatt cagcttcacg atggatgcct
     2941 tgccggggac gccgcagatt caaaacatca ttcccacgac attcttcaac accacgacgt
     3001 gggccgcccc gatactcggg ctcatcggca gcgccttcgt cttcgtcgtc ggtctcgcct
     3061 atatcgaaaa tcgccgccgc gccgcgcaca accacggcga aggctacggc acaaatctgg
     3121 tgaacgaacc ggagccgttc gaggagaagg agctaccgaa tccctggatc gccctcctgc
     3181 cgctcatcgt ggtgggcgtc gcaaacttcg tgctcacctt cgcgataccg cgcgcttacg
     3241 gcgcaaaata cgaggcgatg gtgggcgacc acccgatcgt cacgcagatc ggaccggtgg
     3301 cggcgatatg ggccgtcgaa ggcgcgctga ttctcggaat cctcacggta ttcgcgtttg
     3361 cgtggcggcc cgtgcttgca aagttcgcgg acggcagcaa ggcggcggtc gccggcgcgc
     3421 tgctcgcgtc gctcaatacc gcctcagagt acggcttcgg cggcgtgatc gcgctattgc
     3481 ccggtttcaa ggtagtgtcc agcgcgctca gtgcgatccc caatccgctg gtcaatgaag
     3541 ccgtcacggt gaccgtcctc gccggcatca ccggctcggc ctccggcggc atgagcattg
     3601 ccctggccgc gatgtccgac acattcattg ccgccgcgca cgcagcgcat attccgctcg
     3661 aggtatttca tcgcatcgcg gcgatggcga gcggcggtat ggataccctg ccgcacaatg
     3721 gcgccgtcat cacgttgctt gcagttacgg gactgaccca ccggcaggcc tacaaggaca
     3781 ttttcgtgat cacactgacc aagaccgccg cggtgttcgt cgccatcgcg tggttctatc
     3841 tgactggaat ttactgacgc gggaagggag cgattcgccc ggctccggtt tcccgacgcc
     3901 gattgacgat cgatgggtcg aagcttgtgg aggagttatg gcggttggtg cacgtcgatt
     3961 gacgctcgcg ccggtaaagg cgctggacag tggaaagatc gtcactgcgg aggaggctgc
     4021 cagtctcata cgcgacggtg acacgattgc cacaggcggc tttgtcggga tcggctttgc
     4081 cgaggagatc gccgtggcga tcgaaaagct ctttcttgcg aagggcgagg cgggtccgac
     4141 cggcagcaag ccgggaaatc tgacgctggt ctatgctgcg ggccagggcg acgggaagga
     4201 acggggatta aatcacctag gccatgccgg cctggtgcgc cgagtgatcg gcgggcattg
     4261 gggactggtg ccgaaactgc agcaactcgc catcgccaac cagatcgagg cctacaatct
     4321 tccccagggc gtgatttccc acctgtttcg cgacatcgcg gcgaagcgcc ccggacatct
     4381 gacgcgcgta gggctcggaa cgttcgttga tccgcgacac ggcggcggca agatcaacga
     4441 gcgcaccaag gaggatctcg tccgtgtgat cgacgtcgaa ggcgaggagt tcctgtttta
     4501 ccgtgcgttc ccgatcgaca tcgggattat ccgcggcacg actgccgatc ccgacggaaa
     4561 cgtcacgatg gaaaaggagg cgctgacgct ggaggcactc gccatcgcca tggcggtgcg
     4621 caattcggac gggttggtga ttgcccaggt ggaacggatc gccgatcgcg gctcgctcaa
     4681 cccgcggcag gtgaagatac cgggcatcat ggtcgattgc gtggtcgtcg ccaagccgga
     4741 gcatcactgg cagacgttcg ctgtccagta cgatcccgcc ttcagcagcg aaatccgggc
     4801 tcgcgtggat atgatcccgg ccatggaaat gaacgagcga aagatcatcg cgcgccgtgc
     4861 cgcgctcgaa ctcagcgtca acagtgtcgt caatctcggc atcggcatgc ccgaaggaat
     4921 tgcaaacgtc accaatgaag agaagatttc ggatctggtg acgctcacgg ccgagcccgg
     4981 ggtcattggc ggcgtgccgg ccggcgggct caatttcggc gcggcggtca acgcgcaaac
     5041 cgtgatcgac caaccctatc agttcgactt ctatgacggc ggaggccttg atgcggcctt
     5101 ccttgggctt gcccaggccg accgcgaggg caacctgaat gtcagcaagt ttggcccgcg
     5161 gctcgcgggc gccggaggtt ttatcaacat cagtcagaat gcgaaaaagc tggtgttcgt
     5221 cggcaccttc acggcaggtc ggatgcggct tgcggtcgag aatgggagcc ttcgcatcga
     5281 caaggacggg ggcgtggtca aattcgtccg cgaggtcgag caccgcacgt tctccggtgc
     5341 atacgcgatc cggcgcggtc agcccgtgct ttatgtgacg ga
//
LOCUS       DATLJT010000354         7237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1169149,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000354 DATLJT010000000
VERSION     DATLJT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169149"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="VM910_08235"
     CDS             <1..1892
                     /locus_tag="VM910_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62559.1"
                     /translation="VGVQSPYEAAESVAYLLGNAQAAFKPARINNNFAYCGGGFGGRD
                     HTPFPLYVALAAVFFPNRPVRLANNRFEQFQLGIKRHAFKIRTCIGVDRATGKMVGFA
                     ADHALDGGGLANFSGQVATVGANAALGIYYVPKVDVTTYAFHSRGVTAGSMRGYGTLQ
                     TMTALEVLVDEICTALPLDPIEFRRRNALKPGGRTMAGNPYIVSIRTPEILDKLEQHP
                     IWKERAEHKARGQQAGILVGTGIACATKDYGTGGDGVLGSVEIDPEGRITIHCDYVEM
                     GTGIGTAVANRIAAHLGRVADEIALAQVDTFGPLALVTSGDSYTMSQKTQDAAARNPR
                     WVPAISTASTASIGAHVGTHAAAEAARIIFRFGLWPAALELWGIAPDDPRVNEWKTAR
                     WKDGQLVMPGLPPLALPAVAAKAHARNGVTGAMAHGFNRWAWSQATFVVSGQTWTADI
                     DALAVRRGAGKYVRIDRSNVKFPPTDFNRIGQSYTSQCGHVMRIEIDRATGALRIAKA
                     YSVLECGQALVSELVIGQAQGGFAMSVGYTLLETLPPYEDGPGNGTWNLGQYVVARGS
                     DLPLRDLQVEVLPPVDENERPKGIAEVVMVPVAPALLNAIYDATGHRFRSLPVTQAMI
                     KGALK"
     gene            1889..2410
                     /locus_tag="VM910_08240"
     CDS             1889..2410
                     /locus_tag="VM910_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI62560.1"
                     /translation="MTMLTVTINGRAYGSREVRDELSMNDFLREYLGMTGTKFGCGAA
                     QCLSCAVIIDNPDGTSYTSPTCIVPAVSFNGKSIRTVEGHAKNGELSPLQKAFITHFA
                     FQCGYCTAGFLNESQVLLERLERNPVARADLEKTIAEALDGHLCRCTGYIKYHEAVRA
                     VIVADPGRYLSRA"
     gene            2488..3087
                     /locus_tag="VM910_08245"
     CDS             2488..3087
                     /locus_tag="VM910_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HVI62561.1"
                     /translation="MRITVLAALAALLTGTPTADAVSEQPDSLARPESFAAIADPAAR
                     SAAMFVELGKVLTNPRCVNCHPAGDRPRQGEQGRLHQPPVERGADGHGLPAMHCSICH
                     QRANFDPAGVPGHPEWHLAPREMAWEGKTLAEICAQIKDPKRNGGRSLEELVHHIGTD
                     TLVGWAWAPGFGRKPAPGTQQEAGALAEAWVKTGAGCPN"
     gene            complement(3148..4257)
                     /locus_tag="VM910_08250"
     CDS             complement(3148..4257)
                     /locus_tag="VM910_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62562.1"
                     /translation="MRIDSLLTRCAYATMTAIMLLGLGPAAQAQDPPSFMAPIAGRTA
                     SSPAETATKNVLALNSMMFELYDDAARVFQANILSKHPVILGLFTGAGGRFILYRPGK
                     EPLEAPSVPIVYQLLKSVGHSTMALTEVVGPYLDNAENQSWRSSMAAYRARMKAALDG
                     LDATAMPPDWRGNNRTILENNIAFMDDCLAKGTITLAALQEFAKKQGPYLKHNIHWAA
                     QTQVGHWMSVIGEWKTMLGADWDKTYAASNTIYVARQNNILFSVLAQYFGPEAINERL
                     MLIETISFTSTPDDMLTSLTRIIADRSVGALYFGNYYLMDYELMGGDARAAIIAETEK
                     RGMKPFLPPAVPFGSKQWPTLITPGPGPTSLADVQ"
     gene            complement(4444..5406)
                     /locus_tag="VM910_08255"
     CDS             complement(4444..5406)
                     /locus_tag="VM910_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62563.1"
                     /translation="MQRVAQATGDHAVRARSEAIAFVIMACALVIAPMFAYPVFLMKA
                     LCFALFACAFNLLIGYVGLLSFGHALFFGWASYVGAHAAKVWGFPPELAILSGTATGA
                     GLGAIVGALAIRRQGIYFAMITLALAQMMYFFAIQAPFTHGEDGIQAVPRGKLFGLID
                     LANQTNMYVTVLVIFLGCFLLIYRIINSPFGEVLKAIRENEPRAVSLGYRTERYKLVA
                     FVLSATFAGVAGATKALVFQLASLTDVDWPMSGEVILMTLVGGLGTLFGPVVGAFFMV
                     TLENYLTTIGQWVFVVQGVIFVVCVLLFRRGIIGELAQILRVKL"
     gene            complement(5408..6295)
                     /locus_tag="VM910_08260"
     CDS             complement(5408..6295)
                     /locus_tag="VM910_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62564.1"
                     /translation="MFELLGISSQALFGQLLIGLINGSFYALLSLGLAVIFGMLNIIN
                     FTHGAQYMLGAFAAYFLLLYAGLGYWWAIIIAPILVGITGIIIERLFLQWLYKLDHLY
                     GLLLTFGLALIFEGTFRNFFGSSGLPYSVPPTLQGGQNLGFMFLPNYRAWVVVFSLAV
                     CLTTWFVIERTKLGSYLRAATENPTLVRAFGINVPRMITLTYGFGVALAALAGVMAAP
                     IYNVSPQMGANLIIVVFAVVVIGGMGSIMGAIVTGFGLGIIEGLTKVFFPEASNTVIF
                     IIMAIVLLIRPAGLFGRAT"
     gene            complement(6358..>7237)
                     /locus_tag="VM910_08265"
     CDS             complement(6358..>7237)
                     /locus_tag="VM910_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62565.1"
                     /translation="EIVRDKNKVFLVSGAAASDLTGPKCTPNTIHWTYDTWALANGTG
                     KAIVKTGGDTWFFLTADYAFGHALERDTAAVVEKNGGKVLGKVRHPFPGTDFSSFLLQ
                     AQASKAKIIGLANAGSDTINSIKQASEFGITQGGQNLAALLAFITDVHALGLQTAQGL
                     IMTEAWYWDLNDANREFAKKFAPQFKGVHPTMVHAGVYSAVTDYLKAVEALKSDADGK
                     AIVAKMKEMPTDDKLFGKGTVRADGRKIHPMYLFEVKKPAESKAPWDYYKLRATIPAD
                     EAFRPLADGGCPLVKS"
BASE COUNT         1404 a         2226 c         2320 g         1287 t
ORIGIN      
        1 tcgtcggcgt gcagtcgccc tacgaggccg cggaatccgt cgcctatctg ctcggcaatg
       61 cgcaggcggc tttcaagccg gcacgcatca acaacaattt cgcctattgc ggtggcggtt
      121 tcggcggtcg cgaccatacg ccgttcccac tctacgtcgc acttgctgcg gtgtttttcc
      181 cgaaccgtcc ggtgcggctc gcgaacaacc gcttcgagca gtttcaattg ggcatcaagc
      241 ggcacgcgtt caaaatccgc acgtgcatcg gcgtcgatcg tgcgaccggc aagatggtcg
      301 gatttgccgc cgaccatgca ttggatggtg gaggtctcgc caatttctcg ggccaggtgg
      361 cgaccgtcgg cgccaacgcc gcgcttggta tttactacgt cccaaaagtc gatgtgacga
      421 cgtatgcgtt ccactcgcgc ggcgtgaccg ccggctcgat gcgcggttat ggaaccttgc
      481 agacgatgac cgcgctcgaa gtgctggtcg atgagatctg cacggcgctg ccgctcgacc
      541 cgatcgagtt ccggcgccgc aatgcgctca agcccggcgg ccgcaccatg gcgggaaacc
      601 cctacatcgt gtcgatccgc acgccggaga tcctggataa gctggagcag catccgatct
      661 ggaaggagcg cgccgagcac aaggcgcgcg gacagcaggc gggaattctc gtcggtaccg
      721 gaatcgcttg cgcgaccaag gactacggca ccggaggcga tggtgttctt gggtccgtcg
      781 agatcgaccc cgaaggccgg atcaccatcc attgtgatta cgtcgagatg ggcaccggca
      841 ttggaacagc ggtcgccaat cggatcgcag cgcaccttgg tcgcgttgct gacgaaatcg
      901 cgctcgcgca ggtggacaca ttcggtccgc tggcgctcgt gacctccggc gactcctaca
      961 cgatgagtca gaagacgcag gacgcggcgg cgcgcaatcc acgctgggtg cctgcaatca
     1021 gtacagccag cactgcctcg atcggcgccc atgtcggcac acacgcggcg gccgaggccg
     1081 cgcgcatcat cttccgcttc ggtctatggc cggcggcgct cgaattgtgg ggcatcgcgc
     1141 cggacgatcc tagggtcaac gaatggaaga ccgcgcggtg gaaagacggg cagttggtca
     1201 tgccgggact ccccccactc gcgttgccgg cggtcgccgc aaaagcgcat gcgcgcaatg
     1261 gcgtgaccgg ggcgatggcg catggcttca atcgctgggc atggtcgcag gcgaccttcg
     1321 tggtgagcgg acagacgtgg acggccgaca tcgatgcgct ggccgtgcgc cggggcgcgg
     1381 gcaaatatgt tcgcatcgat cgttccaacg tgaagtttcc gcccaccgac ttcaatcgga
     1441 tcggacaaag ctacacgtcg cagtgcgggc acgtgatgcg catcgagatc gatcgcgcga
     1501 ccggcgcatt gcggatcgca aaggcataca gcgtgctgga atgcggccag gcgttggtct
     1561 cggagctcgt gatcggacag gcgcagggcg gcttcgcaat gagcgttggc tatacgctgc
     1621 tcgagacgct gccgccttac gaggacggac ccggcaacgg gacatggaat ctcgggcaat
     1681 acgtcgtcgc ccgcgggtcg gacctgccgc tgcgcgacct tcaggttgag gtgctgccgc
     1741 cagtggatga gaacgaaagg cccaaaggca tagcggaagt cgtgatggtc ccggtggcgc
     1801 ctgcgctgct caacgcgatt tacgatgcca ccggtcaccg cttccgatcc ttgccggtga
     1861 cccaagccat gatcaaggga gcactcaagt gacgatgctg accgtcacga tcaatggacg
     1921 cgcttacggt tcgcgcgagg tgcgcgacga attgtcgatg aacgattttc tgcgcgagta
     1981 tctcgggatg accggcacca agttcggctg cggcgccgcg cagtgcctga gctgcgcggt
     2041 catcatcgat aatcccgacg ggacgagcta caccagcccc acctgcatcg ttccggcggt
     2101 gagcttcaac ggcaagtcga tccgcacggt ggaaggccat gccaagaacg gcgaactgtc
     2161 gcctttgcag aaggcgttta tcacgcattt tgccttccag tgcggctact gtaccgcggg
     2221 ttttttgaac gaaagccaag tgctgctcga acgtctggag cgcaatccgg tggcgcgggc
     2281 cgacctcgag aagaccattg ccgaggcgct tgacggacac ctgtgccgtt gcaccggcta
     2341 catcaaatat cacgaggccg tgcgcgccgt gattgtggcc gatccaggac gttatctctc
     2401 gcgggcttga gcgaagctcg ggtcgcgacc agcatgatga ttggccgctt ccccgcgatg
     2461 ctggcgatgt tgtgatgggg tgaagaaatg cggatcacgg tcctcgctgc gctcgcggcc
     2521 ctgctgaccg gcacgccgac ggcggatgcc gtttccgaac aaccggatag cctcgcaaga
     2581 cccgagagct tcgcggcgat cgccgacccg gcggcgcgat cagcggcaat gttcgtcgag
     2641 ctcggaaaag tgttgacgaa tccgcgctgc gtgaactgcc atccagccgg cgaccgcccg
     2701 cgccagggcg aacaaggtcg gcttcaccag cctccggtgg agcgcggtgc cgacggtcat
     2761 ggcctaccag ccatgcactg ctcgatctgc caccaacggg ccaacttcga tcccgccggc
     2821 gtgccgggcc acccggagtg gcatctcgcg ccgcgcgaga tggcatggga aggcaaaacg
     2881 ctcgccgaga tctgcgccca gattaaagat cccaagcgca acggcggtcg ctctctggag
     2941 gagctggtgc atcatatcgg cactgacacg ctggttggct gggcttgggc gcccggcttt
     3001 ggccgcaagc ctgctcccgg cacccagcag gaagcgggcg cgctcgctga agcatgggtg
     3061 aaaaccgggg cagggtgtcc gaactaaaac cattccgcga acctgcggcg gtctttgccg
     3121 ggtataccgg ctcgatcgtt tagctcctca ctgaacgtcg gccagcgagg tgggtcctgg
     3181 cccgggcgtg atcagcgtcg gccactgctt cgagccgaac ggcaccgccg gaggaaggaa
     3241 cggcttcatg ccgcgcttct cggtctcggc aatgatggct gcgcgcgcgt cgccgcccat
     3301 cagctcgtag tccatgaggt agtagttgcc gaagtacagc gcgccgaccg agcgatccgc
     3361 gatgatgcgg gtgagcgacg tcagcatgtc gtccggcgtt gaggtgaacg agatcgtctc
     3421 gatcagcatc agccgctcgt taatggcttc cggcccgaag tactgcgcca gcacgctgaa
     3481 caggatgttg ttctgccgcg ccacgtagat ggtgttgctg gcggcgtagg tcttatccca
     3541 gtcggcgccg agcatcgtct tccactcgcc gatcaccgac atccagtgcc cgacttgcgt
     3601 ttgcgccgcc cagtggatgt tgtgcttgag ataagggccc tgcttcttgg caaattcctg
     3661 cagggcggcg agcgtgatcg tgcctttcgc cagacaatcg tccatgaagg caatgttatt
     3721 ctcgaggatg gtgcggttgt tgccgcgcca gtcgggcggc atggccgtgg cgtcgaggcc
     3781 gtcgagggcg gccttcatgc gcgcgcgata ggccgccatc gagctgcgcc aggattgatt
     3841 ctccgcattg tcgagatagg gacccaccac ctcggtcagc gccattgtgc tgtggccgac
     3901 cgatttgagc aattgataga cgatgggcac cgagggcgcc tcgagcggct ccttgccggg
     3961 ccgataaagg atgaagcgtc cacccgctcc cgtgaacaag ccgaggatca ctgggtgctt
     4021 gctcaggatg ttcgcctgga agaccctagc ggcgtcgtcg tagagttcga acatcatgct
     4081 attgagcgcc agcacgttct tcgtcgccgt ctcggccggg ctcgaagccg ttcggccggc
     4141 gatgggcgcc atgaacgacg gcgggtcttg ggcttgggcc gccgggccca gccccagcag
     4201 catgatcgcg gtcatcgttg cgtaggcgca acgggtcagg agcgagtcaa ttcgcatggt
     4261 ttttcctctc aggcacgccg ttgcgtgtgc ctgccaagat ccagcacgtc ggggaacagc
     4321 gaacccgctc ccgtcgtggg cagactcggc gcacgcggtt gccggtcggt tgttgacggc
     4381 tgacgactag caaggtgcgc aaaccggtgc aactgcggct tactggcgct cgcggttgaa
     4441 gctttacagc ttgacgcgca ggatttgcgc aagctcgccg atgatgccgc ggcggaacag
     4501 cagcacgcag acgacgaaaa tcacgccctg gacgacgaac acccattggc cgatcgtggt
     4561 caggtagttt tccagcgtga ccatgaaaaa cgcgccgacg accggcccga acagggtgcc
     4621 aaggccgccg accagcgtca tgagaatgac ttcccccgac atcggccaat cgacgtcggt
     4681 cagggaggca agttgaaaga ccagcgcctt ggtcgcccct gccacccccg cgaacgtcgc
     4741 cgacaatacg aaagcgacga gtttgtaacg ctcggtccta tagccgagtg agaccgcgcg
     4801 gggctcgttc tctcgtatcg ccttgagcac ttcgccgaag ggcgaattga tgatgcgata
     4861 gatcagcagg aagcagccga ggaagatgac tagcacggtg acgtacatgt tggtctggtt
     4921 ggcgaggtcg atcaacccaa acagctttcc gcgcggcacc gcctggatgc cgtcctcgcc
     4981 atgcgtgaac ggcgcttgga tggcgaagaa atacatcatc tgcgccagcg cgagcgtgat
     5041 catggcaaaa tagatgccct gccggcggat cgcgagcgca cccacgatcg cgccgaggcc
     5101 ggcgccggtt gcggttcccg acaggatcgc gagctccggc ggaaagcccc aaaccttggc
     5161 cgcatgcgcg ccgacatagc tcgcccagcc gaagaatagc gcgtggccga acgataacag
     5221 gccgacgtag ccgatcagca gattgaacgc gcaagcgaac agcgcgaagc acagcgcctt
     5281 catcaggaat acgggatagg caaacatcgg cgcgataacg agcgcgcagg ccataatgac
     5341 gaatgcaatc gcctcgctgc gcgcgcgcac ggcgtggtcc ccagtcgcct gcgcaacgcg
     5401 ctgcatgcta tgtggccctt ccaaacagac cggcgggcct gatcaacagc acgatcgcca
     5461 taatgatgaa gatcaccgtg ttcgaagcct cgggaaagaa caccttggtg agaccctcga
     5521 tgatgcccaa tccgaaccca gtcacgatcg cgcccatgat cgagcccatg ccgccgatca
     5581 ccaccacggc gaataccacg atgatgagat tcgcacccat ctgggggctt acgttgtaga
     5641 tcggcgccgc catcacgccc gcaagcgccg ccagcgccac gccgaaaccg taggtgagtg
     5701 tgatcatacg cggcacgttg atgccgaagg cgcgcaccag cgtcggattc tcggtcgcgg
     5761 cgcgcagata ggagccgagc ttcgtgcgct cgatcacgaa ccaggtcgtc aggcacaccg
     5821 cgagcgaaaa caccaccacc caggcgcggt agttcggtaa aaacatgaac ccgagattct
     5881 ggccgccttg cagcgtcggc ggcacggaat agggcagccc cgaggagccg aaaaagttgc
     5941 ggaacgtgcc ctcgaaaatg agcgcgagac caaaggtgag caacagtccg tagaggtgat
     6001 cgagcttata gagccattgc agaaacagcc gctcgataat gatcccggtg atccccacca
     6061 ggataggggc gatgattatc gcccaccaat agcccagtcc ggcatagagc aagagaaagt
     6121 atgcggcgaa cgcgcccagc atgtactgcg cgccgtgcgt gaagttgatg atgttgagca
     6181 tgccgaagat gacggcgagc cccaggctga gcagcgcgta aaacgagccg ttgatgagtc
     6241 cgatcagcaa ctgcccgaac agggcctgcg agctgatgcc gagcagttcg aacatggctg
     6301 atcacatcgt tggccgggga atggaagact tccgctcccc ggcctcgtat gaggacttca
     6361 gctcttcacc agcggacagc cgccatcggc cagggggcgg aaggcttcgt cggccgggat
     6421 ggtggcacgg agtttgtaat aatcccacgg ggccttcgat tccgccggct tcttgacctc
     6481 gaacagatac atcgggtgga tcttgcggcc gtcggcgcgc accgtgccct tgccgaagag
     6541 cttgtcgtcg gtcggcatct ccttcatctt cgcgacgatc gccttgccgt cggcatcgct
     6601 cttgagcgcc tcgaccgcct tgagatagtc cgtgaccgcc gagtagacgc cggcatggac
     6661 catggtcgga tgcacaccct tgaactgagg cgcaaatttc ttcgcgaatt cgcgattggc
     6721 gtcgttcaag tcccagtacc aggcttccgt catgatcaga ccctgagcgg tctgtaaccc
     6781 gagtgcgtgc acgtcggtga tgaacgccag caaggcggcg aggttctgac cgccctgggt
     6841 gatgccgaac tcggacgcct gcttgatgga gttgatcgta tcgctgccgg cattcgcgag
     6901 cccgatgatt ttcgccttcg aagcctgcgc ctgcagcagg aaggacgaga agtccgtgcc
     6961 ggggaagggg tggcggacct tgcccaagac tttgccgccg ttcttctcca ccacggcagc
     7021 agtgtcgcgt tcgagcgcat gaccgaaggc ataatccgcg gtcaggaaga accaggtgtc
     7081 accgccggtc ttcacgatcg ctttgccggt gccgttggcg agcgcccagg tgtcataggt
     7141 ccagtggatc gtattgggag tgcatttggg gccggtcaga tccgaggccg ccgcgccgga
     7201 gacgagaaat accttgttct tgtcgcgtac gatctcg
//
LOCUS       DATLJT010000355         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3466622,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000355 DATLJT010000000
VERSION     DATLJT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3466622"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VM910_08270"
     CDS             complement(<1..585)
                     /locus_tag="VM910_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI62566.1"
                     /translation="MRPALEPVNEATHEIDGKRPTERRLPVVAAIIPCLDEEAAIGQV
                     VTAVLAQNVSEVVVVDGGSRDRTAERAESAGARVIVEPRRGYGRAIQAGIAAVRDDAD
                     ILLFLDGDGSDPTEFISDLVSPIMAGEAVFVLGSRVRGSREPGSLSRQQVVAAYVARC
                     LLRLVYGASFTDMSPFRAIRRDAFQRLGMKEETYG"
     gene            complement(561..1640)
                     /locus_tag="VM910_08275"
     CDS             complement(561..1640)
                     /locus_tag="VM910_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI62567.1"
                     /translation="MSAAAEARGTYAPVDPRRYHESVAGERTEIARRPPVCLYLEVTN
                     RCNLLCTTCPRTYEELEPPADMSWDLFVSIVDQVPDLARAVLHGVGEPMLVASLPRMV
                     GYLKDRGVYVLFNTNGTVLRERNGRALIDAGLDELRVSLDASNRESFKAIRGRDYFGR
                     ILRNVRAFRELQEREGHTTPQVSLWLTGLKETVEQLPSFVKVAAEIGVKEVYLQRLVF
                     FDEAAIGKARPEQALFERLTAGDAVYLKQAEDLAHSLGVTFSASGAASEPGPSLKGSG
                     DGSPWSLCRRPWSLMYFTANGRALPCCIAPFSQHGYGNYTLGHAAHQSLEEIWNGPAY
                     RDFRAALLSDKPPKSCANCGLRWSL"
     gene            complement(1637..>2122)
                     /locus_tag="VM910_08280"
     CDS             complement(1637..>2122)
                     /locus_tag="VM910_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62568.1"
                     /translation="VLGFLTKGYLMEEGISAGNDLWLLSLWRLVFGEHQGDVAAYAAL
                     SALLLIFKGLSVARSSDHTVAARIADINNMLLLTLLLLSPNYPWYFLVMMPFAALCGS
                     PPTWAVSIGALLLSEQLDWDFYLPRMVTKSILFGSLLLAWAFAVLRTRMQTTADTGLS
                     Q"
BASE COUNT          438 a          667 c          654 g          363 t
ORIGIN      
        1 gccataggtt tcctccttca tgccgagacg ctggaatgca tcccgtcgga ttgcgcggaa
       61 cggcgacata tcggtgaagc ttgcaccata gacgaggcgc aaaagacacc tcgcgacata
      121 ggccgcgacg acctgttgcc gtgaaaggct gccgggctcg cgcgaaccac gaacgcgcga
      181 accgaggacg aagacggcct cgcccgccat gatcggcgac accagatcgg agatgaattc
      241 cgtgggatcg ctgccgtcgc cgtcgaggaa gaggaggatg tcggcgtcat cgcgcacagc
      301 ggcgatgcct gcctggatcg cgcgaccgta gccgcgtcgc ggctcgacga tgacgcgagc
      361 tccggccgat tctgcccgct cggccgtacg gtcccgcgag cccccgtcga caacgacgac
      421 ttcgctcaca ttttgcgcca gcacagcggt cacgacctgg ccgatcgccg cttcctcgtc
      481 gaggcacgga atgatcgcgg caacgacggg caggcggcgc tccgttggcc gctttccatc
      541 gatctcgtgg gtcgcctcgt tcacaggctc cagcgcaggc cgcaattggc gcagctcttg
      601 ggcggcttgt ccgaaagcag cgccgcgcga aaatcccgat acgccggtcc gttccagatc
      661 tcctccaggg actgatgcgc ggcatggccg agcgtatagt tgccatagcc atgctgcgaa
      721 aatggcgcga tgcagcacgg caatgcccgg ccattggcgg tgaaatacat cagcgaccac
      781 gggcgccggc aaagcgacca gggtgaaccg tcaccgcttc ctttcagact tggccccggt
      841 tcgctcgccg cgccggaggc gctgaaggtc acgccaaggg aatgcgccag atcctcggcc
      901 tgcttcagat agactgcatc tcctgcggtc aagcgttcga acaaggcttg ctccggacgt
      961 gccttgccga tagctgcttc gtcgaagaac acgagccgtt gcagatacac ttccttgaca
     1021 ccgatctcgg ctgcaacttt cacaaatgac ggaagctgct ccaccgtctc tttcagcccg
     1081 gtgagccaga gggaaacctg cggcgtggtg tgtccctctc gctcctgcag ttcgcgaaac
     1141 gcgcgcacgt tgcggaggat gcggccgaag taatccctgc cacgaatcgc cttgaagctc
     1201 tcgcgatttg acgcatccag cgacacacgc aactcgtcaa gcccggcgtc gatcagcgcg
     1261 cggccattgc gctctctgag gacagttccg ttggtgttga acaggacgta aacgccgcgg
     1321 tccttcagat atccgaccat ccggggcaga cttgcgacca acatcggttc gccgacgccg
     1381 tgcagaaccg cgcgcgcgag atcggggacc tgatcgacaa tcgacacgaa cagatcccag
     1441 ctcatgtctg ccggcggctc gagctcctcg taagtgcggg ggcaggtggt gcacagcaga
     1501 ttgcagcggt tggtcacttc cagataaagg cagaccggcg gacgccgggc gatctcagtg
     1561 cgctcgcccg cgacggactc gtgatagcgc cgcggatcga ccggcgcgta agtacctctc
     1621 gcttcggcgg cagcgctcat tgcgacaacc ctgtgtctgc cgtcgtctgc atgcgggtcc
     1681 tcaagactgc aaaggcccaa gccagcaaga gacttccgaa cagaattgat ttggtcacca
     1741 ttcttggaag gtagaaatcc cagtcgagtt gttcggacag caaaagcgcc ccgatcgaaa
     1801 cggcccacgt tggcggcgag ccacacaggg cggcaaaggg catcatgacc agaaagtacc
     1861 agggataatt gggcgacaac aacaggaggg taaggagtaa catgttgttg atgtcggcaa
     1921 ttctcgccgc gacggtgtga tcggagctgc gggccacgga gagtcccttg aagatgagca
     1981 acagcgcgga gagggcggcg taggctgcga cgtcgccttg atgctcgcca aatacaagcc
     2041 gccagagcga caatagccag agatcgttgc cggcgctgat accctcttcc atcagatagc
     2101 ccttggtcag gaagccgaga ac
//
LOCUS       DATLJT010000356         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1857816,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000356 DATLJT010000000
VERSION     DATLJT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857816"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VM910_08285"
     CDS             <1..709
                     /locus_tag="VM910_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI62569.1"
                     /translation="QKPHPPLWMACSQLPTIERAGQNGFGALGFQFVSADAAHAWVHA
                     YYNAITKRLKKLADYEINPNIALVSFFMCAKTDEEARARADGATFFQFALRYYGASQN
                     RQRPAPGTVNMWDEYNRWKRENPEAQEQALRGGLIGSPETIRKKLRRFRTSHIDQVIM
                     LNQAGKNTHDHICESLELFAKEVMPEFQHDPEHDAWKAGVMSGAIELEEIDTTAFTDR
                     YGKLAVNVAPKVAANGS"
     gene            794..1963
                     /locus_tag="VM910_08290"
     CDS             794..1963
                     /locus_tag="VM910_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI62570.1"
                     /translation="MSSAPRFDIDLAEFWSDPYPALAGMRKEAPIAFVPQLGSTLFCS
                     RDDIFVSEKQIDVFSSHQPQGLMNKLMGHNMMRKDGPAHVAERSVFFPAVSPRAVKSH
                     WIAQFQAHADRIIEALPAAGSIDFVRDFALPFSAECLKSITGLTNMSFQDMNAWSQGM
                     IDGIANYIGDPAVEARCHAATAGIDAAIDDMLPVLSRTPDQSLLGVMVASGMPMASVR
                     ANIKLAISGGQNEPRDAIAGTVWALLTHRDQLAVLRAAEVPWLQAFEEYARWISPIGM
                     SPRRVARPWTIRDISFEPEERVFLMFGSANRDEKHFENADRFDVRRDASKSVAFGAGP
                     HFCAGAWASRAMIADVALPTVFSRLRNLRIVDGEPVRVVGWAFRGLLNLPVTWDR"
     gene            2045..2203
                     /locus_tag="VM910_08295"
     CDS             2045..2203
                     /locus_tag="VM910_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62571.1"
                     /translation="MMKRVATWAFYAIGTLAVLYLALYAYVTFTGRQFTPGDPIHIFR
                     NPNAPSYS"
     gene            complement(2302..3681)
                     /locus_tag="VM910_08300"
     CDS             complement(2302..3681)
                     /locus_tag="VM910_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVI62572.1"
                     /translation="MRLLIVGTLKGQLTTATKIAMENGATVTHAEGSEQAMAVLRGGK
                     GADLLLVDVGLDIRDLVMRLEAEHIHVPIVACGISSDARAAVAAIHAGAKEYIPLPPD
                     PELIAAVLAAVANDSRDLVYRDDAMARVIKLAQQIAGSDASVMITGESGTGKEVLARY
                     LHSRSARAKRPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLL
                     DEISEMDVRLQSKLLRAIQERVIDRVGGTRPVPVDIRIIATSNRNLADAVRDGTFRED
                     LLFRLNVVNLKIPPLRERPADILELAQHFAKKYAEANGVPLRPISAEARRVLTTNRWQ
                     GNVRELENTIHRAVLMALGDEIGPDAILTPDGDRLDVAKTQPAVAHATFAAEQVTRAL
                     VGRTVADVERDLILETLKHCLGNRTHAANILGISIRTLRNKLNEYADVGVPIPPPGTG
                     EYQRAANVG"
     gene            complement(3726..>3947)
                     /gene="fliN"
                     /locus_tag="VM910_08305"
     CDS             complement(3726..>3947)
                     /gene="fliN"
                     /locus_tag="VM910_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HVI62573.1"
                     /translation="DVPVQVSAVLGRSKMDVGELLKLGPGTVLELDRRVGEAIDIYVN
                     NRLVARGEVVLVEDKLGVTMTEIIKAERS"
BASE COUNT          698 a         1239 c         1254 g          756 t
ORIGIN      
        1 ccagaagccg catccgccgc tctggatggc gtgctcgcaa ttgccgacga tcgaacgcgc
       61 cggccagaac ggctttggcg cgcttggctt ccagttcgtg agtgcggatg cggcccacgc
      121 ctgggtgcat gcctattaca atgcgatcac caaacggctg aaaaagctcg ccgactacga
      181 gatcaacccg aatatcgcgc tggtatcgtt cttcatgtgc gcaaaaacgg atgaagaggc
      241 ccgcgcacgc gccgatggtg cgactttctt ccaattcgcg ctgcgttact acggcgcgtc
      301 acagaaccgc cagcggcccg cgcccggcac ggtcaacatg tgggacgaat acaacaggtg
      361 gaagcgggaa aatccggaag cccaggagca ggcgctccgc ggcggcctga tcggctcgcc
      421 ggagaccatc cggaaaaagc tgcggcgctt ccgcacgtcc cacatcgatc aggtcatcat
      481 gctcaaccag gccggcaaga acacgcacga tcatatctgc gagtcgctcg agctgtttgc
      541 gaaggaagtg atgccggagt tccaacacga tcccgagcac gacgcatgga aggcgggcgt
      601 gatgagcggc gcgatcgagc tcgaggagat cgacaccacg gcttttaccg accgctacgg
      661 caagcttgcg gttaatgtcg cgccgaaggt ggctgcgaac ggaagctagc ggggcgcgcc
      721 ttggcgagca ctgcccgcgc tgacgggaac gtggaagcgg cgccaatttc aagcaagatc
      781 gaaagggccg accttgagca gcgctccccg ctttgacatc gaccttgccg agttctggag
      841 cgatccctat ccggcactgg ccgggatgcg caaggaagcg ccgattgcat tcgtaccgca
      901 gcttggcagc acgctgttct gcagccgcga cgacatcttc gtgtccgaga agcagatcga
      961 cgttttcagc tcgcaccagc cgcaaggcct gatgaacaag ctgatgggcc acaacatgat
     1021 gcgcaaggac gggcctgcgc acgtggcgga gcgaagcgtg ttcttccccg ccgtttcgcc
     1081 gcgggccgtg aaatcgcact ggatcgcgca attccaggcg catgccgacc gcatcatcga
     1141 agcactgccg gccgcgggct cgatcgactt cgttcgcgat ttcgcgcttc cgttctcggc
     1201 cgagtgcctg aagtcgatca ccggcctgac caacatgagc tttcaggaca tgaacgcgtg
     1261 gtcgcaggga atgatcgacg gtatcgccaa ctatatcggc gatcccgcgg tcgaggcacg
     1321 ctgccatgcg gcaacggccg gcatcgatgc tgcgatcgac gacatgctgc ctgtcctgag
     1381 caggacgccc gaccagagcc tgcttggcgt gatggtcgcc tccgggatgc cgatggcgag
     1441 cgtgcgggcc aatatcaagc tcgccatctc cggcggccag aacgagccgc gtgatgcgat
     1501 cgcaggcaca gtgtgggcgc tgctgaccca tcgcgaccag cttgccgtgc tgcgcgcagc
     1561 cgaggtgccg tggctgcagg cgttcgagga atatgcgcgc tggatctcgc cgatcggcat
     1621 gtcgccgcgg cgcgtcgcca ggccctggac gattcgcgac atctctttcg agccggagga
     1681 acgcgtcttc ctgatgttcg gctccgccaa tcgcgacgag aagcattttg aaaatgccga
     1741 ccgcttcgac gttcgtcgcg acgcgtcgaa gagcgtggcg ttcggcgcgg ggccgcattt
     1801 ttgtgcaggc gcatgggcct cgcgcgccat gatcgccgat gtcgcactgc ccacggtctt
     1861 ttcccgactg aggaatctgc gcatcgtgga cggcgaaccc gtcagagtcg tcggctgggc
     1921 ttttcgcggc ctcctgaatc ttccggtgac gtgggaccgt tagagatgtc gtcttgcggt
     1981 cgacgcggtc tggccttttc gcaatcgacg atctaaaagc gcgtcatgta ctccagggtc
     2041 ggagatgatg aagcgcgtcg cgacatgggc cttttacgcg atcggcacgc tggccgtgct
     2101 ttatcttgcc ctgtacgctt atgtgacttt caccgggcgc cagttcacgc caggcgatcc
     2161 catccacatc ttccgcaatc ccaacgcgcc cagctattcg tgagatgccg ttgtcattcc
     2221 tgggcacgca tagcgtgaac ggggaatttt cggacttcga gtctggtcgt ttggaccatc
     2281 ccggaatgac gcgtatcttc tttatcccac attcgccgcg cgctgatatt cgccggtgcc
     2341 gggcggcggg atcggcacgc cgacatcggc atattcgttc agcttgttac gtaaagtacg
     2401 gatggaaatg ccgaggatgt tggccgcatg ggtgcgattg ccgaggcaat gcttcagcgt
     2461 ttccaggatc agatcgcgct cgacatcggc gaccgtgcgt ccgaccaggg cgcgtgtgac
     2521 ctgctcggcg gcaaaggttg catgtgccac tgccggctgt gtttttgcga catcgagccg
     2581 gtcaccgtct ggagtgagga ttgcgtccgg gccgatctcg tcgcccagcg ccatcagcac
     2641 cgcgcggtga atggtgttct cgagctcgcg gacattgccc tgccagcgat tggtggtcag
     2701 cacccgtcgt gcctccgccg agatcgggcg cagcggcacg ccgttcgcct cggcatattt
     2761 ttttgcgaaa tgctgcgcca gctcgaggat gtcggccggg cgctcccgca agggcggaat
     2821 cttcaggttc acgacattga gccggaacag aaggtcctcg cggaaagtac catcgcgcac
     2881 ggcgtccgcg agatttcggt tcgaggtcgc gatgatgcgg atgtcgaccg gcacgggcct
     2941 ggtaccgccg acccggtcga tcacgcgctc ctgaattgcg cgaagcaatt tggactgcag
     3001 ccgcacatcc atctcggaga tttcgtcgag cagcagcgtg ccgccggtcg cctcttcgaa
     3061 cttgccgatg cgccgcgcca cggcgcccgt gaaggcgcct ttctcatggc cgaacagttc
     3121 cgactccagg agatgctccg ggatcgccgc gcaattgatc gagatgaacg gtcgcttggc
     3181 gcgggccgag cgcgaatgca ggtagcgcgc gagcacttcc ttgccggtgc cggactcgcc
     3241 ggtgatcatc accgacgcat ccgagcccgc gatctgttgt gcgagcttga ttacccttgc
     3301 catcgcatcg tcgcggtaga cgagatcgcg tgaatcattg gcgaccgccg cgagcaccgc
     3361 ggcgatcagt tcgggatccg gcggcagcgg gatgtattcc ttggcgcctg catggattgc
     3421 ggcgaccgcg gcgcgggcgt cgctcgagat gccgcaggcg acgatcggca cgtggatgtg
     3481 ttcggcctcg agccgcatca cgagatcgcg gatgtcgagc ccgacatcga caagcaggag
     3541 gtccgcgccc ttgccaccgc gcagcacggc catcgcctgc tcggatccct ccgcgtgggt
     3601 cacggtcgcg ccgttctcca tggcgatctt ggtggcggtc gtgagctggc ccttcagggt
     3661 accaacgatg agaagccgca tgtgtgtctc ctgttcgtct gtgcgcgtct ggcgcgcgtc
     3721 gtaaatcagc tacgttcggc cttgatgatt tccgtcatgg tcacgcccag cttgtcttcc
     3781 accagcacca cctcgccgcg cgcgaccaga cggttgttga cgtagatgtc gatggcttcg
     3841 ccgacgcgcc ggtctagttc gagcacagtg ccgggcccga gcttcaagag ttcgccgacg
     3901 tccattttcg aacgtcccag cacggccgag acctgcacgg gcacgtc
//
LOCUS       DATLJT010000357         1513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_709241,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000357 DATLJT010000000
VERSION     DATLJT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709241"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1513)
                     /locus_tag="VM910_08310"
     CDS             complement(<1..>1513)
                     /locus_tag="VM910_08310"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HVI62574.1"
                     /translation="VLDYDFVWKGKSTAGLLRDTGESVYARSPRQGFADHAPGEYQAT
                     VELARAAFARGDLFEAVPGQLFAEPCERSPAEVFQRLCRINPSPYGALMNLGDGEFLV
                     SASPEMFVRSDGKRVETCPISGTIARGTDAIGDAEQIRQLLNSEKDEFELNMCTDVDR
                     NDKARVCVPGTIKVLARRQIETYSKLFHTVDHVEGMLRPGFDALDAFLTHAWAVTVTG
                     APKLWAMQFVEDHERSSRRWYAGAIGCVNFDGSINTGLTIRTIRMKDGLAEVRVGATC
                     LFDSDPAAEDRECQVKAAALFQALRGDPPKPLSSTAPDATGSGKQVLLIDHDDSFVHM
                     LADYFRQVGASVTVVRYVHALSMLKQRKWDLLVLSPGPGRPEDFKISSTIDAAIENKL
                     PIFGVCLGLQAIGEYFGGQLGQLAQPAHGRPSRVQVRGGRLMRSLPNVIVIGRYHSLY
                     VERGTMPDVLDVTASTEDGVAMAIEHKTLPVGAVQFHPESLMSLSGDVGLRIVE"
BASE COUNT          280 a          502 c          471 g          260 t
ORIGIN      
        1 ctcgacgatg cgcaagccca cgtctcccga aagcgacatc agcgactccg ggtgaaactg
       61 cacggcgccg accggcagcg tcttgtgttc gatggccatc gccacaccgt cctcggtcga
      121 ggccgtgaca tcgagcacat cgggcattgt gccgcgctcg acataaagcg aatggtagcg
      181 gccgatcacg atcacgttcg gcaagcttcg catcagccgc ccgcccctca cctgcacgcg
      241 cgacgggcgg ccatgcgcgg gttgcgcgag ctggccgagc tggccgccga aatattcgcc
      301 gatcgcctga agtcccaggc aaacgccgaa aatcggcagc ttgttctcaa tcgcggcatc
      361 gatggtgctt gaaatcttga aatcctccgg ccggcccggc cccggcgaca gcacgagaag
      421 gtcccatttt ctctgcttca gcatggacag cgcatgcaca taacgcacca cggtgacgct
      481 ggcgccgacc tggcgaaaat aatccgccag catatgcacg aagctgtcgt catggtcgat
      541 caagagcacc tgcttgccgc tgccggtggc atcgggcgcg gtgctcgata acggcttcgg
      601 cgggtcgccg cgcagcgcct gaaaaagcgc cgccgccttg acctggcatt ccctgtcctc
      661 cgcggcgggg tcggaatcga acaggcaggt ggccccgacc cgcacttcgg caagcccgtc
      721 cttcatgcgg atggtgcgga tcgtgagccc cgtgttgatg ctgccgtcga aattgacgca
      781 gccgatggca ccggcatacc agcgtcgcga cgagcgctcg tgatcctcga cgaactgcat
      841 cgcccagagt tttggggcgc cggtcaccgt caccgcccag gcgtgggtaa ggaaggcatc
      901 gagcgcatca aagcccgggc gcagcatgcc ttcgacatga tcgacggtgt ggaacagctt
      961 cgaataggtc tcgatctgcc gccgcgccag caccttgatc gtgccgggga cgcagacgcg
     1021 cgccttgtcg ttgcggtcga cgtcggtgca catgttgagc tcgaactcgt ccttctccga
     1081 gttgagaagc tgccggatct gttcggcgtc gccgatcgca tcggtgccgc gcgcgatcgt
     1141 gccggagatc ggacaggtct cgacccgttt gccatcggat cgtacgaaca tttccggcga
     1201 cgccgagacc aggaattcgc cgtcgccgag attcatcaac gcgccatagg gcgacgggtt
     1261 gatgcggcag agccgctgga acacttccgc cggcgagcgc tcgcaaggct cggcgaagag
     1321 ctgccccggc acggcctcga acaggtcgcc tcgggcaaac gccgcgcgcg caagttcgac
     1381 agttgcctga tattcgccgg gcgcgtgatc ggcaaatccc tgccgcggcg acctcgcata
     1441 gacgctttcg ccggtatcgc gcaaaaggcc tgcggtcgat ttgcccttcc agacgaagtc
     1501 ataatcgagc acc
//
LOCUS       DATLJT010000358         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2088182,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000358 DATLJT010000000
VERSION     DATLJT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088182"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VM910_08315"
     CDS             complement(<1..209)
                     /locus_tag="VM910_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HVI62575.1"
                     /translation="MKPEPLTDAELERLSAILERFGDKRSMNLEKLDGFIAALVCGPD
                     IILPSEYLPEIWGGDIVLEGTFAAQP"
     gene            complement(460..1452)
                     /locus_tag="VM910_08320"
     CDS             complement(460..1452)
                     /locus_tag="VM910_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62576.1"
                     /translation="MSSRRDFITLLGGAAAAWPLAARAQQSERMRRIGVLMGVLDDRE
                     GQARVTALKQGLQELGWTDGRNIQIETRFGGADVGRIRTHAAELVALGPDVIVAHTTP
                     VIRAVRQATSSIPIVMATVNDPVEQGFVSSLAHPGGNITGFMFIDFQMVGKWLEMLKE
                     AAPGVSRAILMFNPDTAPYYYLYLRSFEAEPRSIAVEVTAAPFRNTAEIEAAIAKLGR
                     ELGGGLIVAPDGFTGVHQHLFIRLAQQYRVPAVYSFRTYVAEGALMLYGPDAYDVIRR
                     SASYVDRILKGAKPADLPIQQPTKFELAINLKTAKALGLQIPDKLLAIADEVIE"
     gene            complement(1449..2432)
                     /locus_tag="VM910_08325"
     CDS             complement(1449..2432)
                     /locus_tag="VM910_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62577.1"
                     /translation="MKRRQFITLVGGAAAWPLAARAQQAAMPVVGLLSPQSRGAASQL
                     LEAFRRGLAETGYVEGQNVAIEYRLTEGRFDRLAEMAADLVRRQVSAISVPGGSVATL
                     AAKAATQTIPIVFGVAEDPVKLGLVASLARPGGNLTGINFFTAEVVAKRLGLLRELLP
                     SAARIAVLVNPTNATNAEATVRDVQSAAPAMGLQIQIFNASTNSEIDASFATLVRERA
                     DALFVAPDAFFTARRVQLVHLATRHTIPATYPVREFVEAGGLMSYGTSITDMYRHVGV
                     YTGRILKGAKPADLPVVQSSKFELIINLQTAKLFGLNVPAMLLASADGVIE"
     gene            complement(2429..3427)
                     /locus_tag="VM910_08330"
     CDS             complement(2429..3427)
                     /locus_tag="VM910_08330"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62578.1"
                     /translation="MTVTIGRREFIALLGGAAAAWPLAARAQQGGKVYRIGFAANDPT
                     IPITAAGAAFVEGLRENGFVEGKNIVIERRFAEGRSDRAVALIAELVRDNIDLLVTSG
                     AQIHPAAKQATTKIPIVMVNATDPVGEGLVASLARPGGNITGLVQVPSAELAGKRIQL
                     LKDAAPQISRVAVLMNPDFSSDHSQWSALERAAQSLGITLLAVSARRGSELADALAKS
                     IGEHPDALFAMNNGLNLTYRKVIVDFATKHRLPLMHAFTEATRDGGLISYETDRPALF
                     RHAAVLVGKILKGANPGDIPIEQPTRFELAINLRTARTLGLTIPRDLLLLADEVIE"
     gene            complement(4254..>4739)
                     /locus_tag="VM910_08335"
     CDS             complement(4254..>4739)
                     /locus_tag="VM910_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HVI62579.1"
                     /translation="ESAPARIITTASQAHQGAHIPFDDLNAERSYRGFGRYCETKLAN
                     ILFTIELARRLDGTGVTANCFHPGLVATGFNRNNGLLTNLGMAILRPVARSPEKGAET
                     LVWLATAPHVANVSGAYFFDQEQRPPSPEAQDMETAGRLWEISKRQCAADDPSASVCQ
                     R"
BASE COUNT          881 a         1470 c         1452 g          936 t
ORIGIN      
        1 ggctgcgcag cgaaggtgcc ttcaaggacg atatcgcctc cccaaatctc tggaagatat
       61 tcgctcggga ggatgatgtc gggaccgcat actagagcgg cgatgaagcc atccagtttt
      121 tccagattca tcgaacgctt atcgccaaag cgttcgagga tcgcgctcag gcgttctagc
      181 tctgcatcgg tcaacggttc aggtttcacc gctcacctgc ccccacgctt gctgcccaga
      241 atagtccgcc gccggttgct gagtgcgact caatggcatg cggcgaacta tgctgctggc
      301 gacaaaacac agcaagtggg caagaaccga gcaacaaatt gatcacagaa aggacctttc
      361 atggcccttc ccgtcgatgt gcaggccgct cctgcgcgcg cgccgcgagc ggccacgccg
      421 ccgcagcgcc gccgagcagg gtgatgaact ggcgccgctt cattcgatca cctcgtcggc
      481 aatagcgagc agcttgtctg gaatttggag gccgagcgcc ttggcggtct tgaggttgat
      541 ggccagttcg aacttcgttg gctgctgaat gggaagatca gccggcttgg ctcccttcaa
      601 gatgcgatcg acatacgaag ctgagcgccg gatcacgtcg taggcatcgg gtccatacaa
      661 catcaaggcg ccttccgcga catatgtccg aaaggagtac acggccggca ctcggtactg
      721 ctgcgccagc ctgatgaaca agtgctgatg gacacccgtg aacccgtccg gcgcaacgat
      781 cagcccgccg cccaactcgc ggccaagctt ggcgatagcc gcttcgattt cggcggtgtt
      841 gcgaaagggg gccgccgtca cctcaactgc gattgaccgg ggctcggcct cgaatgaacg
      901 caggtagagg tagtaatagg gggccgtgtc cgggttgaac atgagtatgg cgcgagaaac
      961 gccgggagca gcctctttga gcatctccag ccacttcccc accatttgga aatcgatgaa
     1021 catgaagcca gtgatgttcc cgcccggatg cgccaagctc gaaacgaagc cctgctcgac
     1081 gggatcgttg accgtcgcca taacgatcgg aatagagctg gtcgcctgcc gcaccgcccg
     1141 gatgactggc gtggtatggg cgacgatcac gtcgggtccg agcgccacta attctgctgc
     1201 gtgagtgcgt atgcggccga cgtcggcccc accaaagcgt gtctcgattt ggatgttgcg
     1261 gccgtctgtc catcctaact cttgaagtcc ctgcttaagc gctgtcactc gggcctgtcc
     1321 ttcccgatcg tccaacaccc ccatcagcac cccgatccgc cgcatccgct cagactgttg
     1381 cgcgcgtgcc gcgagcggcc acgcggccgc cgcgccgccg agcagcgtga taaaatcgcg
     1441 acggctgctc attcgatcac cccgtcggcg ctggcgagca acattgcggg cacgttgaga
     1501 ccgaacaatt tggccgtctg caggttgatg atcagctcga acttgctgga ctgcacgacc
     1561 ggaaggtccg caggcttcgc gcccttgaga atgcggccgg tgtacacgcc gacgtggcgg
     1621 tacatatcgg tgatgctggt gccgtaactc atcaacccac cggcctcgac aaattcacgc
     1681 acagggtagg tcgcggggat cgtgtggcgc gtcgccaagt gtactaattg gacccgccgg
     1741 gccgtgaaga atgcatcagg agcgacgaac agggcgtcag cccgctcacg cacaagcgtt
     1801 gcgaacgacg catcgatctc gctgttggtg cttgcattga agatttggat ttgcagcccc
     1861 atggcaggag ccgccgattg cacatcccgc acggtggcct cggcgttcgt cgcatttgtc
     1921 ggattgacca gcacggcaat gcgagcggcg ctgggcagga gctcacgcaa gagccccagc
     1981 cgctttgcta ccacctccgc ggtgaaaaaa ttgatccctg tcaggttgcc gcccggccgg
     2041 gcgagactgg cgacaagacc aagcttgaca gggtcctcgg cgacgccgaa cacaattgga
     2101 atcgtctgag tagcagcctt ggctgcgagc gtcgccaccg agccgcctgg caccgaaatc
     2161 gcgctcactt ggcggcggac cagatcggct gccatctccg caagccgatc gaagcgccct
     2221 tccgtcaagc gatactcgat cgccacgttc tggccctcga cgtaacctgt ttcggcgagg
     2281 ccccggcgga atgcctccaa gagctgggac gcagctccgc gcgactgggg actgaggagc
     2341 cccaccaccg gcatcgccgc ctgctgcgcc cgcgccgcga gcggccacgc cgccgcgccg
     2401 ccgaccagcg tgatgaactg gcggcgtttc attcaatcac ctcgtcggcg agcagcagga
     2461 gatcgcgcgg aatcgtcaac ccgagcgtcc ttgccgttct caggttgatc gccagctcga
     2521 atcttgtcgg ctgctcgatc ggaatgtcgc cgggattggc ccccttgaga attttgccca
     2581 ctaatacggc ggcgtgtctg aacagtgcag gccggtccgt ttcgtaggat atgaggcctc
     2641 cgtcccgcgt cgcctcggtg aatgcgtgca ttaacggcag ccgatgcttg gtcgcaaaat
     2701 cgacgatgac cttgcggtag gtcaagttga gaccgttgtt catcgcgaac agtgcgtctg
     2761 ggtgttcacc tattgatttc gcgagagcgt cggccaattc gctaccccgt ctcgccgaga
     2821 ccgcgagcaa tgttatgccc agggattgcg ccgcacgctc cagcgcgctc cattgcgaat
     2881 gatcggagga aaagtccggg ttcataagga cggcaactcg cgaaatctgg ggtgcggcgt
     2941 ccttgagcag ttgaatgcgt ttgccggcaa gctccgcgga gggcacttgc acaaggccgg
     3001 tgatgttgcc ccccggtcgc gccaagctcg cgacgagccc ctcgccgacc gggtcggttg
     3061 cgttcaccat aacgatggga atcttcgtgg tggcctgttt cgcggcggga tggatctgcg
     3121 cgccggatgt aacaaggagg tctatgttgt cgcggactag ttccgcaatc agagcaacgg
     3181 cccggtcgct gcgtccttcc gcgaaccgac gttcaatgac gatgtttttg ccttcgacga
     3241 atccattctc ccgcaatcct tcgacgaagg ccgcacccgc cgcggtgatc ggaattgtcg
     3301 ggtcgttggc cgcaaagccg atccgatata cttttccgcc ctgctgcgcc cgcgccgcga
     3361 gcggccacgc ggccgccgcg ccgccgagca gtgcaatgaa ctcccgccgt ccgatggtga
     3421 ctgtcatacg cccctcccgc cggaggtcgt gtgcatgcaa tagaggggac gatatcacgc
     3481 tttagcgaag gaacgaacaa tgcttttgcg ctgcgaaagt cttgagccgc ccatgtcgca
     3541 gatgggtcaa actcggtcat tcggtgatgt tggtcgcatg aacgcgcgga gctgatggac
     3601 gggctcgacg agcttgagca ttgtgctcgg ctgctgaaag gcagtctcgg gaagctccac
     3661 taagatcgca tcgtgaccgc tgatgtgtcc ccactcgact cgtggggaca cctcacctca
     3721 acaacttcaa caagttggag ctgaagggac gattcagaac aattcaggtc tgccccaagg
     3781 actagccggc gcgcccattg caggctgagg atgccgtggt ccgcccactg ggaggtggtc
     3841 gcatggtcga cttgaggcgc cgcgcgttca tcacgctgct cggcggcgcg gctgctgcat
     3901 ggccgctcgc cattggttcg tgcggcagtg atcggtcgtc aggtggaatc tttgactgag
     3961 gtgtatacgc agaattagaa ggagggatga ctagcgcctc tggtgcacct ccgccatcgt
     4021 tatcgcgggc tgcaattgag cacgtaagga acgggcggaa tccgacgtca tttgtatttt
     4081 gatggggaca atcgagggct ctttgccggg cgtgattacc tccagcgtca tctctacgac
     4141 tgcgccttcg gcgcgcgccg tgacatgctg cgcaattggt aatgcatacg gagggctgcc
     4201 ctcgaactta atgtttccca tggatcgttc cttgttgcat ctcccgctgc ttctcaccgc
     4261 tggcacaccg aggcgctcgg gtcatcggcc gcgcactgcc gcttgctgat ctcccacagc
     4321 cgccccgcag tctccatgtc ctgtgcctcg gggctcggcg gcctttgctc ctggtcaaag
     4381 aagtatgccc cgctcacgtt cgcaacgtgt ggcgcggtgg caagccatac aagggtttct
     4441 gctcccttct ccgggctacg tgccaccggc ctgaggatcg ccatccccaa gttcgtcagc
     4501 aggccgttat tgcggttgaa tcctgttgcc acgagcccag ggtggaagca gttcgccgtc
     4561 accccggtcc catcgagacg tcgcgccagt tcaatcgtga acaggatgtt ggccagcttg
     4621 gtttcgcagt agcggccaaa gccccgatag gagcgctcgg cgttgagatc gtcgaacggg
     4681 atgtgcgctc cctgatgcgc ctgggatgcc gtggtgatga tgcgggccgg cgcgctctc
//
LOCUS       DATLJT010000359         4045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1857938,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000359 DATLJT010000000
VERSION     DATLJT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857938"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VM910_08340"
     CDS             <1..108
                     /locus_tag="VM910_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVI62580.1"
                     /translation="RVIVPIPGTSSVAHLDENVAAARILLSTEDLHELS"
     gene            complement(645..1925)
                     /locus_tag="VM910_08345"
     CDS             complement(645..1925)
                     /locus_tag="VM910_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVI62581.1"
                     /translation="MLSAIDTRPALAAIDQRRVLELEQRSIRTPSSTFEEGDIADLYA
                     DYMSAMGLEVEMQQVTHPFDAKRRSRQPIGRLKGTGGGPTLLINGHMDPGVEMSGWTV
                     DPYAAKFEDGWIWGMGAHDDKGGCVAAICALEAIVRSGIRLKGDVLMTPVVAHKLGGA
                     GTRALLRHGVKADLCINMEHSNNTIANVCVGVVMVRFVCSAPELFFRYSPQARAAYIN
                     PIEQQMEIVRRIGPSLTPIAAGGWMTFTPHPELPGFPTHTFDVMHKEHYYFKSATGRS
                     TRECELTLQFRTVPGQTVATIDSDLKRLLARIKQDHPAFNCVLEVPARGTEQTWNQLA
                     MVCPADHPLVAALAEGQTLGSGQSAVVGAHGRLGNVGDGNIIQEVLGIPTVQYGPGDI
                     RAYKEWPTPDERVRLSDLVTAAKAVAHATIRLCS"
     gene            complement(1949..3211)
                     /locus_tag="VM910_08350"
     CDS             complement(1949..3211)
                     /locus_tag="VM910_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62582.1"
                     /translation="MRFRIVTLACVAMLSAAASAQAQQEVKIGVLDPLSGPTAQIGID
                     AVAAIKTAAEIVNEGADLPLTLARNKGLPGLGGAKVTLVVVDHQGKPEVGQSETERLI
                     TQEKVQALIGAYFSSVTAAGSQAAERAGVPFINGDSTQPALTQRGLKYFFRTTPTDEN
                     FSELMFDFLKDFAAKSGQKFQSVSLFHEDTAYGTDSAKVQERLAGERGFRVLEKIAYK
                     AQTTSLTAEVQRLKAANADVLMPSSYTSDTFLLLRTAKDLDYNPKLIVAQNAGFTDPT
                     FISTMGKDAEGAITRSPYNADLESHIPLLPKINAIFKKHSNGRDLSDVPARNFTAAMT
                     LFDAINRAGSTDPEKIRAALAATNIPPDQLIVPYRGVRFATTGQNELVRAILMQVQKG
                     KYCTIYPFELASCQVLYPTPTWAEKAKL"
     gene            3459..>4045
                     /locus_tag="VM910_08355"
     CDS             3459..>4045
                     /locus_tag="VM910_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62583.1"
                     /translation="MSRELILQAVISGLLMGLIYALVAVGLTLIFGLMEIVNFAHGEF
                     LMLAMFTAFWFWALAGLDPVVSLPFVVVILALAGLLAHFGIIRRLLKAPMLAQVCGTF
                     GLGVALRAAAQFLWTPDFRTVTNPVIAGRVQIGSIFIGQPQLITGGVCLLSFAALWWF
                     VTRTETGLGLQATAQDREAAAVMGIPIERMFALGWA"
BASE COUNT          697 a         1201 c         1286 g          861 t
ORIGIN      
        1 cgcgtcattg ttcccattcc cgggacatca tcggtggcgc accttgacga gaacgtcgcc
       61 gccgcgcgga ttctcctttc gaccgaagat ctgcacgaac tgtcctgaaa cggttgcttc
      121 tggcaacgcg ggtgccgaag ctattggctc atctcggctg gcaaacacga ctctgaggac
      181 gctagtcgtt ccggaactaa agctgttccg cccacatcgg gagccgatca cccaatcaga
      241 cgatgtgatc gacgaaccgc cttttggcga agggcgagtc tcggaacgga gctgatcgtt
      301 cgggcgagcg ccacgcccct gctcgcggcc gctcgcgctt tagtcgcgct aaccatgtag
      361 aatgtcgtga attttttgag gtgccgtata tgagccacag agagcggacg gcgtggctgg
      421 gctgacagga ttcgaactca caaatgcgac gcgcaaaccg cgcctttgag atgtcggagg
      481 aatttggccc gcttgcggaa cacctggtga ccagagactt ttcgcgtaaa ggagccagcc
      541 tttgacttgc cacttcgagc tgcggtgagt gcccggtaca tagaattcga acccgtctca
      601 gccggggtgt ttcggatatt tcgctttaga aagcctaacg tacctcagct gcacaatctg
      661 atcgtcgcat gcgcgaccgc tttcgcggcg gtgacgaggt cgctgaggcg cacccgctcg
      721 tcgggcgtcg gccattcctt gtacgcgcgg atgtcgcccg gaccatattg cacggtcggg
      781 ataccgagca cttcctggat gatattgccg tcaccgacat tgccgagccg cccgtgcgcg
      841 ccgaccacag ccgattggcc cgagcccaaa gtctggccct ccgccagcgc ggcgacgaga
      901 gggtgatcgg cagggcagac catcgccagt tggttccagg tttgctcggt gccgcgtgcg
      961 gggacttcga gcacgcaatt gaacgccgga tgatcctgct tgatgcgcgc caagagccgc
     1021 ttgagatcgc tgtcgatggt ggcgaccgtc tggccgggaa cggtgcggaa ctgcaaggtc
     1081 agctcgcatt ctcgcgtcga gcgcccggtg gcgcttttga aataataatg ctccttgtgc
     1141 atcacgtcga aagtgtgggt cggaaagccg gggagttccg gatgcggcgt aaaagtcatc
     1201 catccaccgg cagcgattgg cgtcaggctc ggaccgatcc gccgcacgat ttccatctgc
     1261 tgctcgatgg gattgatgta ggcagcgcgc gcttgcgggc tgtaacggaa aaacagctcg
     1321 ggggccgagc aaacgaagcg caccatcacg acaccaacgc aaacattggc gatcgtattg
     1381 ttggagtgct ccatgttgat gcacaggtcc gccttgacgc cgtggcgcag gagcgcgcga
     1441 gtgccggccc cgccaagctt gtgagcaacg accggggtca tgagaacgtc gcccttgagc
     1501 cgaatgcctg accgtacgat cgcctccagc gcacaaatcg cggctacgca accgcccttg
     1561 tcatcgtgcg cgcccattcc ccagatccaa ccgtcctcga attttgcggc gtagggatcg
     1621 acggtccacc cgctcatctc gacgccggga tccatatgac cgttgatgag aagcgtcggc
     1681 ccgcccccgg tgcctttgag ccggccgatc ggctggcggc tcctgcgctt cgcgtcgaat
     1741 ggatgcgtta cctgctgcat ctcgacctcg aggcccatag ccgacatgta gtcggcatag
     1801 aggtcggcga tgtcgccctc ctcgaacgtc gagctcggcg tccggatcga gcgctgctct
     1861 agctcgagaa cacgcctctg gtcgatcgcg gcaagcgccg gcctcgtatc gattgcggac
     1921 agcactgctg gtctgcgcgg cttgcggctt acaacttcgc tttttcggcc caggtcgggg
     1981 tcggataaag gacctgacac gacgcaagct cgaacggata gattgtgcag tatttgccct
     2041 tctgcacctg catcaagatc gcccgcacca gctcgttctg gccggtcgtt gcgaacctga
     2101 cgccgcggta gggcacgatc agctggtcgg ggggaatgtt ggttgccgca agtgcggcgc
     2161 gaatcttttc gggatccgtg gagccggcgc ggttgatcgc atcgaacagg gtcatggcgg
     2221 cggtgaagtt gcgagctgga acgtccgaaa gatcacgccc gttcgaatgc ttcttgaaga
     2281 tggcgttgat cttgggaaga agcggaatgt ggctttcgag gtcggcgttg tacggagagc
     2341 gggtgatcgc cccctccgcg tccttgccca tggtgctgat gaaggtcggg tcggtgaacc
     2401 cggcgttctg cgccacgatg agctttggat tgtagtccag atccttggcg gtacgcagga
     2461 gcaggaacgt gtccgacgta taggacgacg gcatcagaac gtcggcgttg gcggccttga
     2521 gccgctgcac ctccgcggtg agcgacgtcg tctgggcctt gtaggcgatt ttttcgagca
     2581 cgcggaaacc gcgctccccg gcaagccgtt cctggacttt tgcgctgtcc gtgccgtagg
     2641 cggtatcctc gtgaaagaga gacacagatt ggaatttctg gccggacttg gccgcgaaat
     2701 ccttcaagaa gtcgaacatc agctcgctga agttctcgtc cgtcggggtc gtgcggaaga
     2761 aatacttcag tccgcgctgc gtcagcgccg gctgggtgga atctccattg atgaagggca
     2821 ctcctgcgcg ctcggcggct tggcttcccg cggcggtcac cgaggagaag taggcgccga
     2881 tgagggcttg gaccttctcc tgcgtgatga gacgctcggt ttcgctttgg cccacctccg
     2941 gcttgccctg atggtcgacc acgacgaggg tcactttggc gccaccaaga cccggtaagc
     3001 ccttgttcct ggcgagagtc agcgggagat cggcgccctc gttgacgatc tcggcggccg
     3061 tcttgatcgc cgcgacggcg tcgatgccga tctgcgcggt cgggcccgac agcggatcga
     3121 gcacgccaat cttgacctct tgctgtgcct gtgccgatgc tgcggcgctc aacattgcga
     3181 cgcatgcaag agtgacgatt ctgaatctca tggcggtgct ccattcgcct ttattcgtac
     3241 gattagctga agttcctatc gtgggggcgg cgaactcagg caggggttcg aatgcaaaac
     3301 cgcgcgacaa tgatagagtt gctgagtgcg tctgcttcca aagctcgcca tcgcaggtgc
     3361 gaccaacgct cgtgaatgcc ggggtcgctc gtctgattgg agcgcttttt gcatgtgctc
     3421 gtcaatcggt tcagctgttt tgaggggcgc gcgcatcgat gtctagagag ctcatcctgc
     3481 aagccgtcat cagtgggctg ctgatggggt tgatctatgc gcttgtggcg gtcggcctga
     3541 cgctgatttt cggccttatg gaaattgtca acttcgcgca tggtgagttt ctcatgctcg
     3601 ccatgttcac ggcattctgg ttctgggctc ttgccggcct cgatccggtg gtgtcgctgc
     3661 cgttcgtggt cgttatcctc gcccttgccg gcttattggc gcacttcggc atcatccggc
     3721 ggctgttgaa ggcgcccatg ctcgcccagg tctgcggcac gttcggcctc ggtgtcgcgc
     3781 tgcgcgccgc cgcgcaattc ctctggacgc ccgactttcg caccgtcacc aatccggtca
     3841 ttgccggacg cgtgcaaatc ggctcgatct tcatcggcca gccgcagctg atcacgggag
     3901 gcgtctgcct gttgtcgttc gccgcgctgt ggtggttcgt cacgcgcact gaaaccggtc
     3961 tcggcctgca ggcgaccgcg caggatcgcg aggcggcggc cgtgatgggg attccgatcg
     4021 agcgaatgtt cgcattggga tgggc
//
LOCUS       DATLJT010000360         4200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3696477,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000360 DATLJT010000000
VERSION     DATLJT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696477"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VM910_08360"
     CDS             complement(<1..205)
                     /locus_tag="VM910_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI62584.1"
                     /translation="MATNHLAPATGNIADNVVGFARALRAAGLPVGPGAVIDAMNALQ
                     VIDIGNRRDVYTTLESIFVKRHEH"
     gene            complement(320..1228)
                     /locus_tag="VM910_08365"
     CDS             complement(320..1228)
                     /locus_tag="VM910_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVI62585.1"
                     /translation="MTASALPASVDAMLDLLTSRGYLAERSLATVTYLSLRMGRPLFL
                     EGEAGVGKTEIAKVLAAALGRKLIRLQCYEGLDVASAVYEWNSAAQMIAIRVAEAAGE
                     HDREQLSADIFADRFLIKRPLLQALEPDVGGSPVLLIDELDRADEAFEAYLLEILSDF
                     QITIPEFGTVKAPKPPIVIITSNRTREIHDALKRRCLYHWVDYPSAERELAIIKNRVP
                     GISAKLSQQVVRFVQALRDQDFYKSPGVAETIDWATALSELDARSLTPEVVGDTLGAL
                     LKYQDDIARLQGDGLQKVLKEATSET"
     gene            complement(1266..2072)
                     /locus_tag="VM910_08370"
     CDS             complement(1266..2072)
                     /locus_tag="VM910_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HVI62586.1"
                     /translation="MYEFKYHRPATVRQAANLLAKNEDAKVIAGGHTLVPVMKQRLAS
                     PPHLVDLSHIEGLDTIEMKGRSLVIGATARHAEVARSAVVGEAIPALAELAGMIGDPA
                     VRHRGTIGGSLANNDPTADYPAAVLALGATIVTNKRRLKPEEFFQGLFTTALESDEII
                     TRVMFPLPKKAAYVKFRNQASRYALVGVFVAKRPSDVRVAVTGAGSNGVFRLTSFEEA
                     LKKRFSHKVLDGLSVPADGLNSDLHGSAEYRAHLIAVLARRAVEAATAKA"
     gene            complement(2111..2596)
                     /locus_tag="VM910_08375"
     CDS             complement(2111..2596)
                     /locus_tag="VM910_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI62587.1"
                     /translation="MAKISLIVNGNPVTANVDPRTLLVQFLRENLRLTGTHVGCDTSQ
                     CGACVVHLDGKAVKSCTTLAVMADGHEVKTIEGLAPDGAPLHPMQEAFREHHGLQCGF
                     CTPGMIMTAVDMVHRLGHELSDEVIREELEGNLCRCTGYHNIVVSIAAGAKAMAKSDL
                     A"
     gene            complement(2739..3185)
                     /locus_tag="VM910_08380"
     CDS             complement(2739..3185)
                     /locus_tag="VM910_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HVI62588.1"
                     /translation="MAMTMNGEVQLPASRQTVWTKLNDPEVLKACIPGCEELEETDEH
                     GFRAVAKMKVGPVSARFKGKVNLRDLDPPNGYKISGEGEGGVAGFAKGGATVKLSEKD
                     GGTLLSYDVEAQIGGKLAQLGQRLINGAAKKLADEFFTNFAKAVEG"
     gene            3352..>4200
                     /locus_tag="VM910_08385"
     CDS             3352..>4200
                     /locus_tag="VM910_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVI62589.1"
                     /translation="MSTSLSPLLAPLLSSSAMRGVCDDATALQHMLDFEKALARAEAA
                     CGVIPERAVDPIARACKASSFELAALAQAATRAGNLAIPLVKALTAEVAKSDTEAARY
                     VHWGATSQDVIDTAAMLTLRAGVDALLWDLDRAIEGFAKLARKHRHTPVVARTWLQHA
                     LPMPFGLKLAEYAAALHRSRLRLKRVRAEKLALQFGGAAGTLAALGDKGLMVAEKLAA
                     ELELPLPEAPWHTHRDRIADAAAALAIVAGSCGKIARDVSLLMQTDVGEAFEPSSEGR
                     GGSSTMP"
BASE COUNT          737 a         1355 c         1338 g          770 t
ORIGIN      
        1 catgctcatg gcgcttgacg aagatcgatt ccagcgtggt gtagacgtcg cggcgattgc
       61 cgatgtcgat cacctgcaac gcgttcatgg cgtcgatgac ggcacccggc ccgaccggga
      121 ggccggcggc acgcagcgca cgggcaaagc cgaccacatt gtcggcgata ttgccggtcg
      181 caggcgcgag atgatttgtt gccatcgaac accccggttc acgtcgtttc ccgcggcgac
      241 gcgtcggcgt cgaacccgga ataagattac ggcgcgagat tccgggttcg gccctctgcg
      301 ccgccctgga atgacaaagt cacgtctcgc tcgtcgcttc cttcaaaacc ttctgcaggc
      361 cgtcgccctg caagcgcgcg atgtcgtcct ggtatttgag cagcgcgccg agcgtatcgc
      421 cgaccacctc cggggtgagc gagcgcgcgt cgagctcgga aagcgcggtc gcccagtcga
      481 tggtctcggc gacgccgggc gatttgtaaa aatcctggtc gcgcaacgcc tgcacgaagc
      541 gcaccacctg ctgcgacagc ttcgccgaga tcccgggcac gcggttcttg atgatcgcga
      601 gctcgcgctc ggcactgggg tagtcgaccc aatgataaag gcagcgtcgc ttcagcgcgt
      661 catggatctc gcgcgtgcgg ttcgaggtga tgatgacgat cggcggcttc ggcgccttca
      721 cggttccgaa ttcgggaatc gtgatctgga agtccgaaag gatttccagc aggtacgcct
      781 cgaaggcttc gtcggcccgg tccagttcgt cgatcaggag caccgggctg ccgccgacgt
      841 ccggctccag cgcctgcagc agcggccgct tgatcagaaa gcggtcggca aagatgtcgg
      901 ccgacaattg ctcgcggtcg tgctcgccgg cggcttcggc gacgcggatc gcaatcatct
      961 gcgccgcgct gttccactcg tagacggcgg aggcgacgtc gaggccttca taacactgca
     1021 ggcggatcag cttccgcccc agtgcggccg ccagcacctt tgcgatctcg gtcttgccga
     1081 cacccgcctc gccctccagg aacaaggggc ggcccatgcg gagcgacaga tacgtgacgg
     1141 tcgcaagcga ccgttcggcg agatagccgc gcgaggtcaa gaggtcgagc attgcatcga
     1201 ccgatgcagg cagtgccgat gcagtcatca agcgaaacgc ctttgtgcgc cggagaacgg
     1261 caatcttatg cttttgcggt cgcggcttcg accgcgcggc gggccagtac cgcgatcaaa
     1321 tgcgcgcgat attcggcgct gccgtgcagg tcgctgttca ggccgtctgc cggcacgctc
     1381 agcccgtcga gcaccttgtg cgagaaccgc ttcttcagcg cctcctcgaa cgaggtcagg
     1441 cggaacacgc cattcgaccc tgcgccggtg acggccaccc gcacgtcgga gggccgcttg
     1501 gcgacgaaca caccaaccaa cgcataacgc gaagcctggt tgcgaaattt gacgtaggcc
     1561 gccttcttcg gcagcgggaa catgaccctc gtgatgatct cgtcggactc cagcgccgtc
     1621 gtgaacagcc cttgaaaaaa ctcctcgggc ttgaggcggc gtttgttggt gacgatggtg
     1681 gcgccgagcg caagtacggc tgcgggatag tcggccgtcg gatcgttgtt ggcgagcgag
     1741 ccgccgatcg tgccgcgatg gcgcaccgcg gggtcgccga tcatccctgc gagttccgcc
     1801 agcgcgggga tcgcttcgcc cacgacggcg gaccttgcga cttcggcatg gcgcgcggtc
     1861 gcaccgatca ccagcgagcg gcccttcatc tcgatcgtgt cgaggccctc gatatgggag
     1921 aggtcgacca gatgcggcgg gcttgcgagc cgctgcttca tcaccgggac cagcgtgtgg
     1981 ccgccggcga tcaccttcgc gtcttcgttc ttggcaagca gattggccgc ctgccgcact
     2041 gtcgccggac gatgatattt gaattcgtac atggggtttc ctgaccgcga ggtcgctggt
     2101 acgcgttact ttatgcgagg tcggacttcg ccatcgcctt ggcgccggcg gcgatcgaga
     2161 cgacgatgtt gtgatagccg gtgcagcggc agaggttgcc ttcgagctct tcgcgaatga
     2221 cctcgtcgga cagttcgtgg ccgagccgat gaaccatgtc gacggcagtc atgatcatgc
     2281 ccggggtgca gaagccgcac tgcaggccgt gatgttcgcg aaacgcttcc tgcatcggat
     2341 gcagcggcgc gccgtcggga gcgaggcctt cgatcgtctt cacctcgtgc ccgtcggcca
     2401 tcaccgcaag cgtggtgcag gacttcaccg ctttgccgtc gagatgcacc acgcaggcgc
     2461 cgcattgcga cgtgtcgcag ccgacatggg taccggtcag ccggagattc tcgcggagga
     2521 actgcaccag cagcgtgcgg ggatcgacat tggcggttac gggattaccg ttcacgatga
     2581 gggaaatttt ggccatcaca ctctctttat ctgcgctgcc gcattgcacg gcaaagcagt
     2641 ctcaaaatca atcaaacgcc ataatatgag cccgtcctgc aatgggcaac ctcacccagg
     2701 acatcacatt gcttggctcg caaagcctta ggcaggagct aaccctccac agcctttgcg
     2761 aaattcgtaa agaactcgtc cgccagtttc ttggccgcgc cgttgatcag ccgctggccg
     2821 agctgcgcca gcttgccgcc gatctgcgcc tccacatcat aggataacag cgtgccgccg
     2881 tccttctcgc tgagcttgac ggttgcaccg cccttggcga agccggcaac gccgccttcg
     2941 ccttcgccgg atatcttgta gccgttgggc ggatcgagat cgcgcaggtt gaccttgccc
     3001 ttgaagcgcg ccgacaccgg tccgaccttc atcttcgcca ccgcgcgaaa tccgtgctcg
     3061 tcggtctcct ccaactcctc gcagcctgga atgcaggcct tgagcacctc cggatcgttg
     3121 agcttggtcc aaaccgtctg acgcgaggcc ggaagctgga cttcgccgtt catagtcata
     3181 gccatgagga cctccccgca cactcgctgc cgtgtccccc aagtaagcca cgccgcaagg
     3241 aaaagaaagg gtggtttggg acgatgtgcg atgcataatc gacggctgcg aggactttgc
     3301 gcgcctgaaa ggattggcac aggcgggcga gaagtgatta ggtcgcgcgg catgagcaca
     3361 tccctttctc ccctcctcgc ccccctgctg tcgagcagcg cgatgcgcgg cgtctgcgac
     3421 gatgcgactg cgctgcagca tatgctcgat ttcgaaaagg cgctggcgcg cgccgaagca
     3481 gcctgcggcg tcatacccga acgcgcggtc gatccgattg cgagggcctg caaggcgagt
     3541 tcattcgaac ttgccgcgct tgcccaggcg gcgacccgcg cgggcaatct cgcaatcccc
     3601 ctggtgaagg cgctcaccgc cgaggttgcc aagtccgata cggaggccgc gcgctatgtg
     3661 cattggggcg ccaccagtca ggacgtgatc gataccgccg ccatgctgac cttgcgcgcc
     3721 ggcgtcgacg cgctgctttg ggatctcgat cgcgcgatcg aaggctttgc gaagcttgcg
     3781 cgcaaacatc gtcacacgcc ggtggtggcg cgcacctggc tgcagcatgc gctgccgatg
     3841 ccgtttggct tgaaactggc ggaatatgcc gcagcactcc atcgctcgcg gctgcgactg
     3901 aagcgcgtac gcgcggagaa gctggcgctg caatttggcg gcgcggccgg aacgctcgcc
     3961 gcgctcggcg acaagggatt gatggtggcg gaaaaactcg cggcggaatt ggagctgccg
     4021 ctgccggagg caccctggca cacccatcgc gaccgcatcg cggacgctgc cgccgcgctc
     4081 gccatcgttg ccgggagctg cggcaagatc gcgcgcgacg tctcgcttct gatgcagacc
     4141 gatgtcggcg aagccttcga accatcgagc gaagggcgcg gcggctcctc caccatgccg
//
LOCUS       DATLJT010000361         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2548246,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000361 DATLJT010000000
VERSION     DATLJT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2548246"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1216
                     /locus_tag="VM910_08390"
     CDS             50..1216
                     /locus_tag="VM910_08390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI62590.1"
                     /translation="MDFALSPEQQQIRDEVRKLCVQFDDAYWLEKDRSGEFPYELHAA
                     LAAAGWLGIAMPEEYGGAGLGITEAVIMMQAIAESGAAMSGASAVHINIFGLQPVVVF
                     GTDEQKCRMLPPLIAGKDKACFAVTEPDAGLDTTAIKVRAERRGDHYLLSGQKIWIST
                     AQVANKILILARTTPLDRVKRKTEGLSLFYTDLDRSRVAVRVIDKMGRRAVDSNEMFF
                     DGMVVPAADRIGAEGEGFRSILHGMNPERILIAAEAVGIGLAALRKAASYARERVVFR
                     RPIGQNQAIAHPLAQCWMELEAANLMVMKAATLYDRKLDCAAEANAAKYLAAEAAFHA
                     CETAVLTHGGMGYSKEFHVERYLREVMIPRIAPITPHMILNFIAEKILGLPKSY"
     gene            1253..2161
                     /locus_tag="VM910_08395"
     CDS             1253..2161
                     /locus_tag="VM910_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVI62591.1"
                     /translation="MAFLRRIGLVATVVAALVAWPGAWQCAWAEGAGGPAKKSPRSAC
                     DHAAFRVVVDVGHTAESPGAPSARGFWEYDFNLRLATLIKQKLLAAGFAKTVLLVTDG
                     PARKSLFQRVARANRLSADLFLSIHHDSVPDSFLETWEYEGQEHGFSDRFKGHSIFFS
                     NENVDPAASLLFGQLLGEQLQARGLQYTPHYLERFMGHRQRLLVDAKVGVYRYDQLIV
                     LRTTQMPAVLLEAGSIINRGEELVMNSPERRSLIGAAVTDAVEGFCAIRRPRNPDRVA
                     SPAGAAAKQTSSPDATAQPADTAKAR"
     gene            2316..>2653
                     /locus_tag="VM910_08400"
     CDS             2316..>2653
                     /locus_tag="VM910_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI62592.1"
                     /translation="MSIPKPVYNPPFNVTRASHSVLTVKDLAVSRNFYVDLLGFIVSD
                     EDREALYLRGVAEACHHSLVLKRAHGEPQAERVGMRVFSDEDLEKAKAFFDKAGLPAT
                     WVEVPHQGRTL"
BASE COUNT          488 a          847 c          839 g          479 t
ORIGIN      
        1 gcgcgcaaat ccgctacaat ggcggtgtcg caagcggtga ggttggacaa tggattttgc
       61 attgagcccg gagcagcagc agatccggga cgaggttcgc aagctctgcg ttcaattcga
      121 cgacgcgtat tggctggaga aggaccgcag cggcgagttt ccgtacgagc tgcatgccgc
      181 gctcgccgcg gccggttggc tcggcattgc gatgccggaa gaatacggcg gagccggtct
      241 cggcatcacc gaagcggtga tcatgatgca ggcgatcgcc gaatccggtg ccgccatgag
      301 cggagcgtcg gcggttcaca tcaatatctt cggcctccag ccggtcgtcg tgttcggaac
      361 cgatgaacag aagtgccgca tgctgccgcc gctgatcgcc ggcaaggaca aggcctgctt
      421 cgccgtgacc gagcccgacg ccggtctcga taccacggcc atcaaggtgc gcgccgagcg
      481 ccgcggcgat cactacctgc tgtcgggcca gaagatctgg atttccaccg cgcaagtcgc
      541 caacaagatt ctcattctcg cgcgcaccac gccgctcgac cgcgtcaaac gcaagaccga
      601 ggggctgagc ctgttctaca ccgatctcga tcgcagccgc gtcgcggtgc gcgtcatcga
      661 caagatgggc cgccgtgcgg tcgattcaaa cgagatgttt ttcgacggaa tggtcgtccc
      721 cgcggccgat cgcatcggcg cggaggggga gggctttcgc tcgatcctgc acggcatgaa
      781 tccagaacgc atcctcatcg ccgcggaggc ggtcggcatc ggtctcgctg cgttgcgcaa
      841 agccgcgagc tatgcccgcg agcgtgtggt ctttcggcgg cccatcggcc agaatcaggc
      901 gatcgcgcat ccgctcgcgc aatgctggat ggagctggaa gcagccaacc tgatggtgat
      961 gaaggcggcg accctctacg accgcaagtt ggattgcgcg gccgaggcca atgctgccaa
     1021 gtatctggcg gcggaggccg ccttccatgc ctgtgagacc gccgtcttga cccacggcgg
     1081 catgggctat tccaaagaat ttcatgtcga acgctacctg cgcgaggtca tgatcccgcg
     1141 gatcgcgcca attacgccac atatgatcct caatttcatt gcagagaaga ttctcggcct
     1201 gccgaagtcc tattagggac gcactggcgg ccaggggctc ggggtaggtt tgatggcttt
     1261 tcttcggcgg atcggactgg tcgcgaccgt cgttgctgcg ctcgtggcgt ggccgggcgc
     1321 atggcaatgc gcgtgggccg aaggcgccgg tggtccggcg aagaagtcgc cacgttcggc
     1381 ctgcgaccat gccgcctttc gcgtcgtcgt ggacgtcgga cacacggccg agtccccggg
     1441 tgcccccagc gcgcgcggct tctgggaata tgacttcaac ctgcggctcg cgacgctgat
     1501 caaacaaaaa ttgctcgctg ccggctttgc caagaccgtc ttgctggtca ccgatgggcc
     1561 ggccaggaag agcctgttcc agcgcgtggc gcgggccaat cggttgtcgg ccgacctctt
     1621 cctctcgatc catcacgact cggtgccgga ctcgtttctg gaaacctggg agtacgaagg
     1681 ccaggagcac ggcttcagcg accggttcaa aggccactcg atcttcttct ccaacgagaa
     1741 tgtcgatcct gctgccagcc ttctgttcgg ccagctgctt ggggagcagc tgcaggcccg
     1801 tggtctgcaa tacactcccc actacctcga aagattcatg ggccaccggc aacgactgct
     1861 ggtggatgcc aaggtgggtg tctatcgata cgatcagctg atcgtgctga ggacgaccca
     1921 gatgcccgcg gtcttgctcg aggccggctc gatcatcaat cgcggcgagg aattggtgat
     1981 gaattcgccg gagcgccgat cgctcatcgg cgcggccgtg accgatgcgg tcgaaggctt
     2041 ttgcgccata cggcggccgc gaaatcccga tcgcgttgcc agcccggcgg gcgccgccgc
     2101 caaacaaacg tcctctcccg acgccacagc gcagccggcg gacaccgcca aagctcgctg
     2161 atcacgccaa caacaggggc cacgatctcg gtgccgtccc tctcccccgc aaacgggaag
     2221 aggaggcaag ccgagttcgt cgcccgcgcc gattccattt cacagaaacg tgctctagaa
     2281 cggtttccca ttcgacatcc cagttggagc gcgccatgag cattccaaag cccgtctaca
     2341 atccgccctt caacgtcacg cgcgcgagcc actcggtgct caccgtgaag gacctggcgg
     2401 taagccgaaa cttctacgtg gatctcctcg gcttcatcgt cagcgatgaa gacagggaag
     2461 cgctctatct gcgcggcgtc gccgaggcgt gtcatcacag cctcgttctc aagcgcgcgc
     2521 acggcgagcc gcaggccgag cgcgtgggca tgcgcgtctt cagcgacgag gatctggaga
     2581 aggccaaggc gtttttcgac aaagccggct tgccggcgac atgggtcgag gtcccgcacc
     2641 aggggcgcac cct
//
LOCUS       DATLJT010000362         1694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3237546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000362 DATLJT010000000
VERSION     DATLJT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3237546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /gene="paaA"
                     /locus_tag="VM910_08405"
     CDS             complement(<1..702)
                     /gene="paaA"
                     /locus_tag="VM910_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HVI62593.1"
                     /translation="MYTQALNTSETDDRHVEDATHAASFQARIDAEERIEPNDWMPAA
                     YRKTLTRQISQHAHSEIVGMLPEGNWITRAPSLRRKAALLAKVQDECGHGLYLYAAAE
                     TLGTSREELVDAMLAGKAKYSSIFNYPTLTWADIGTIGWLVDGAAIMNQIPLCRCSYG
                     PYARAMIRVCKEESFHQRQGFEIMLTLCRGTEGQKAMAQDALNRWWWPVLMMFGPPDK
                     ASQHSDTSTKWKIKRF"
     gene            complement(776..1645)
                     /gene="paaX"
                     /locus_tag="VM910_08410"
     CDS             complement(776..1645)
                     /gene="paaX"
                     /locus_tag="VM910_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HVI62594.1"
                     /translation="MPQPLDRIIEELKREPSRTGSIVITVFGDAIVPRGGSVWLGTLL
                     EFFKALDIDGGVVRTAVSRLASDGWLERNRVGRNSFYRLAATGRHTFDIATRHIYDPP
                     PQDWTGRFELLLIGNADDRDASREALRSAGFGSPLPGVWVAPSGVPIPDEASRAIRLE
                     VSAEDDSGRRLLADSWPLENIADAYLKFMKTFEPLRSWITRRAPLANGDAFAARILLI
                     HHYRRVVLRDPLLPLALLPKDWPGRAARALCGEIYRAILPASEQWLDRNGSNEDGPLP
                     KAGSELGNRFRDA"
BASE COUNT          313 a          537 c          522 g          322 t
ORIGIN      
        1 aaagcgcttg atcttccatt tggtcgacgt gtcgctgtgc tggctcgcct tgtccggcgg
       61 accgaacatc atcaagaccg gccaccacca gcgattcagc gcatcctgcg ccatcgcctt
      121 ctgcccttcg gtgccgcggc acagcgtcag catgatttcg aaaccctggc gctggtgaaa
      181 ggactcttcc ttgcagacgc ggatcattgc ccgcgcgtag ggtccgtagg agcaacggca
      241 caacggaatc tgattcatga tcgccgcgcc atcgaccagc cagccgatcg ttccgatgtc
      301 ggcccaggtc agcgtcggat agttgaagat ggaggagtac ttcgccttgc cggccaacat
      361 tgcgtcgacc agttcctcgc gcgaagtgcc gagcgtttcg gccgctgcgt agagataaag
      421 gccgtggccg cattcgtcct gcactttggc aagcagcgcc gctttccggc gcagggacgg
      481 cgcgcgagtg atccagttgc cctccgggag catgccgacg atttcggaat gggcgtgctg
      541 cgagatttgc cgcgtcagcg tcttgcgata ggcggccggc atccagtcgt tcggctcgat
      601 gcgctcctcg gcatcaatcc gcgcctgaaa cgaagcggcg tgcgtcgcat cctccacatg
      661 tcggtcatcg gtctcagacg tattcagcgc ctgggtgtac atgccgatct tcctccgatt
      721 tgttggccgc aacatataac acattcttgc gaatgcaagt catttttgta acatatcagg
      781 cgtcgcggaa tctgttcccg agttcggagc ctgccttggg caagggaccg tcttcgttgc
      841 ttccgttacg gtcaagccat tgttccgatg cgggaagtat ggcgcgatag atctctccac
      901 agagcgcgcg cgccgccctg cccggccaat ccttcggcag cagcgccaac ggcagcaagg
      961 gatcgcggag caccacgcgg cggtaatgat ggatcaggag aatgcgcgcg gcgaaggcgt
     1021 cgccgttcgc aagcggcgcg cgtcgcgtga tccagctacg caacggctcg aacgttttca
     1081 tgaacttgag ataggcatcc gcgatgtttt cgagcggcca actgtcggca aggagccgcc
     1141 ggccgctgtc atcctcggcg gagacttcga gacggatcgc acgcgaagcc tcgtccggga
     1201 tcggcacacc cgacggcgcg acccaaacgc ccggcagcgg actgccgaac cccgcactcc
     1261 tcaatgcctc gcgcgaagcg tcgcggtcgt ccgcattgcc gatcaacagc aactcgaaac
     1321 ggccggtcca gtcctgcggc ggcgggtcgt agatgtgtcg ggtcgcgata tcgaaggtgt
     1381 gccggccggt cgcagccagc cgataaaagc tgttgcggcc gaccctgttg cgctcaagcc
     1441 agccgtcgct cgcaagacgg gacaccgcag tgcgtaccac gccgccgtcg atgtcgagcg
     1501 ccttgaagaa ttcgagcagc gtgccgagcc agaccgagcc gccgcgcggc acgatggcat
     1561 cgccgaaaac ggtgatgacg atggagccgg tgcgcgaagg ttcgcgcttg agctcttcaa
     1621 tgattcgatc aagcggctgc ggcatgctga tgtcccgctt ctaacatccg catgccatcg
     1681 cggcgagcac ttat
//
LOCUS       DATLJT010000363         9803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_250525,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000363 DATLJT010000000
VERSION     DATLJT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250525"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            619..1278
                     /locus_tag="VM910_08415"
     CDS             619..1278
                     /locus_tag="VM910_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI62595.1"
                     /translation="MERPARQPAPSEEPIVFVVDDDESMREALRSLLRSVRLRVETFG
                     STADLLRSKLPDVASCLVLDVRLPGLSGLDFQAELAKANIRIPIIFMTGHGDIPMSVQ
                     AMKAGAVDFLTKPFRDQDMLDAVAAALERDRNRRKDEQQLSDLRARFEALTERESEVM
                     GLVTAGLMNKQIAGELGLSEITVKIHRGHVMRKMAARSLADLVRMAEAIGVRQAKPEN
                     R"
     gene            1466..2494
                     /locus_tag="VM910_08420"
     CDS             1466..2494
                     /locus_tag="VM910_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI62596.1"
                     /translation="MARHINTSEIEMERAPIAGTSLEVSRVALGTWAIGGWMWGGTDE
                     AESIATIRAAVERGINLIDTAPAYGFGRSEEIVGKAIAEGHLRSQVLIATKTGLAWEG
                     GRVFRNASRARILQEVEDSLRRLRTDHIDVYQVHWPDPLVTIEETAEAMQSLFKQGKI
                     RAIGVSNFSVRQMERFRRVAPLHVLQPPYNVFERGIEADTLPYCRKNNIATFGYGALC
                     RGLLSGRIGLDSRFQGDDLRRTDPKFQQPRFGQYLAAVERLDQLAQRRFRKRVIHLAV
                     RWALDQGITTALWGARHADQLQPVDEVSGWKLDAAAKAEIDRILKTTIKDPVGPEFMA
                     PPASNVAA"
     gene            2692..3072
                     /locus_tag="VM910_08425"
     CDS             2692..3072
                     /locus_tag="VM910_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62597.1"
                     /translation="MQTPPLISIIDDDESMRCAVKSLVTSLGLNAYTFASAEEFLRSP
                     HVEHTSCLISDLQMPGLSGVELQNTLLAQDRRIPIIFMTAFPEERMRTRAMKDGALGF
                     LSKPFESQMLINLIEKAIGISRKE"
     gene            3458..4552
                     /locus_tag="VM910_08430"
     CDS             3458..4552
                     /locus_tag="VM910_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI62598.1"
                     /translation="MLSQSLRTNSARTNSAPGPRFHNGVPYGRLGSFVLRNAMLRSVD
                     EHARLNEGELRPYLPSKLSHFHSGAVMDQAVGIFPSDAVARQGLAWGGMAVEVVQAIT
                     HERVEFRFRGFQHLLVVHEEGVRASGETLIEGLPRSTLRDVKRKLAFVPAGHEYRETQ
                     EPRTRSRVVYIYFDPAKMRRSPGPVLTERPLAPRLFFEDKALWETAVKLGRLIESGTE
                     DRHYREALGIVLAHELVHRNDGAQRIEPPARGGLAGWQQRITAAYIEEHLAEPIALAT
                     LARSVRLSAYHFCRAFKRSFGVPPHRYHNARRIEHAKTLLAEPTCSVTGIALKIGFSE
                     TSSFTAAFRKATGTTPTAYRRSLASLARPS"
     gene            complement(4605..5066)
                     /locus_tag="VM910_08435"
     CDS             complement(4605..5066)
                     /locus_tag="VM910_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62599.1"
                     /translation="MTPVARHGAGLRPGLLSSVDPIGMVGTPSCELAGVEVLDWKDVM
                     LPVAEAGPQLVAIGPGGVAPVMSPTPLLSNGAVEVDVVVIAPAVPGLEHAVDKPSPLD
                     EPELTPGVASGAAPKGIPVGAIAVPELLMPKGEVMPMPTLGIKGVIPPPCA"
     gene            5120..6298
                     /locus_tag="VM910_08440"
     CDS             5120..6298
                     /locus_tag="VM910_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI62600.1"
                     /translation="MRSKVIISTISAVVVAAIVGALVVTHRFPSKAASASTPAVPTAP
                     VVAGAVTSQDVPIYLQGVGTVIAYNTVIVRSQIQGQITKVNFTEGQAVKTGDLLAVID
                     PRPYQAQLDQATANRSRDQAQLTNAESNLKRYTTLGNQGWATPQLVETQTAQVAQLNA
                     AIKADEAAIEWAQTQLSYTQLTSPIDGVTGIRQVDIGNIIHPTDPNGLVVVTQVEPIS
                     VIFTLPETSLPQIQQQLADGSLKVEAYSQDNTMKLDEGSLDFIDNEIVQTTGSVRFRA
                     NFPNKEHHLWPGELVNAWLLLDTRHDGLTVSASAVQQGPQGPYVYVINADNTVAVRPV
                     KVAQVSGGRALIDSGLAAGEQVVVDGQYKLQPGIHVTILHGKAAEEAAAQEAQQTPIP
                     "
     gene            6295..9426
                     /locus_tag="VM910_08445"
     CDS             6295..9426
                     /locus_tag="VM910_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI62601.1"
                     /translation="MNISAPFIARPIATALLMVGLLLGGLAAYPLLPIAALPNVNYPT
                     LQITAQLPGADPQTMASSVATPLETQFGQIPGLTQMTSASALGYTQITLQFDLSRQID
                     GAVADALSAINAASSYLPPNLPYPPTINKVNPADTPILVLALTSDTLPLTTVDAYAEN
                     ILLQKISQISGVGLVGIAGRQKPAIRIQVDPQALAARGLSLEDVRSVIGVDNVDLPKG
                     TLNSPRQTYILNTNDQLFKPEAYADLIIAYRNGSPVRVKDVGKAISGPENNLIAAWFN
                     QKRAIILPIQRSPGANVIATVNRIKAMMPVLQASIPSGIKIDTVSDRTETIRASVTDV
                     QFTLVLSVALVVMVIFLFLRNVWATIIPAVTVPLSLIGTVAVLYELGYSLDNLSLMAL
                     TIATGFVVDDAVVVIENTVRHLEEGLSPMQAAIKSAGEIGFTIISITLSLIAVFIPLF
                     LMSGYVGALFREFAVAVSVSLLLSLVIALTLTPMMCSRLLKPESKEHGRIYRLFERGF
                     DALLGAYEAGLKVVLRHRLITLMVMLGTLALTGYLFIVIPKGFFPQQDTGLIIGFSEA
                     AQDISYQAMAERTQALIKAVLEDPAVASVGTGIGAGGGNYTTNTGRMFISLKPKNQRD
                     ASADQVILRLQQRLAKIQGIALYMQAAQDITIGGRLNKTQYQYTLADSDPGELNHWAP
                     LFLEKIRALPGITGVTTDQLNAGPLLDITINRDVASSYGITPSTIDNILNDAYGQRIV
                     STMYTQMSQYHVVMEVPPKYQYDPEALGQLYVSSSSGQQVPLRTLITSVVKVSPLVVN
                     HQGQFPSVTISYNLFPGTSLGEAVASIQQVEKELGKPLSLQTSFQGNGQAYLQSLSST
                     PILILAALVVIYLILGILYESTVHPITILSTLPSAGIGALLLLMASGFDMSVIAIVGI
                     LLLIGIVKKNGIMLVDFAIHGERNEGLGPEEAIYQACIKRFRPILMTTMAAMLGAVPL
                     MLGTGTGSEIREPLGYAIVGGLALSQFLTLYTTPVVFLYLDRLTRRHPRRPAASDGSA
                     APKPHPAVPREAQ"
     gene            9477..9803
                     /locus_tag="VM910_08450"
                     /pseudo
     CDS             9477..9803
                     /locus_tag="VM910_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757903.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         1969 a         3128 c         2884 g         1822 t
ORIGIN      
        1 gccctgtacg gccggcgcaa gggccggccg tactctcaaa gcaacattag gtgcgggcta
       61 cgaacgaatt cttcgcccaa tcgccgaagt cgatgaccgc tagctgttgt ccggaatccc
      121 ggggcttgcc agcgatactg cgcgctttga ccgcaggcgc gccaagtggc cttacggata
      181 gcagggggag cttggtccaa acccctggcc gtaacagttc gccgcactgt tcccggcggg
      241 cgccgtctgc gcgaacgtcg ggctggcaac gagggtggcc aatgcgagag cagcgataag
      301 tgccttcatg atctctctcc atgtgtgcgg acgattgtcc atggtttcct gcaacagcct
      361 cggcccgacg ccccgtgcct tgcagttacc tgggagatag cctcgtagcg ggcacgggat
      421 gaactatatt ctgggtgttg cggcataacc gaaggatcga cgcgcacata ctttggttta
      481 agctttcgcc gaaattcatg cgcgaggagg aacggaacag ccgtcgggtt tgttgtcgcg
      541 gaggtccgct gaccggctcg ctaatccgtg cggaagtgct atctattgtg gcaaggcttg
      601 cccggagagt acgccgtgat agagcgtccc gctcgtcagc ccgctcctag cgaagagccg
      661 atcgtattcg tcgtcgacga cgatgaatcc atgcgcgaag cgctcagaag ccttcttcga
      721 tcggttaggc tcagggtcga gacgttcggt tccaccgcgg acttgttgcg aagcaagctt
      781 cccgatgtgg cgagctgtct cgtgctggat gtcagattgc cggggctgag tgggctcgat
      841 ttccaagccg agctcgccaa ggcgaacata cgcattccga tcatattcat gaccggccat
      901 ggcgacatcc ccatgtcggt ccaggccatg aaagcggggg cggtcgattt tttgaccaag
      961 ccttttcgcg atcaggacat gctggatgcg gtcgccgccg cactcgagcg cgaccgcaac
     1021 cggcgaaaag acgagcagca gctttcggac ctgcgggcgc gcttcgaagc gttgaccgag
     1081 cgcgaaagcg aggtgatggg cctggtcacc gccggcctga tgaacaagca gatcgccggc
     1141 gagctcgggc tgagcgagat caccgtgaag atccatcgcg ggcacgtcat gcgcaaaatg
     1201 gccgcgcgct cgcttgcgga cctggtgcgg atggcggaag cgataggtgt gcggcaggcc
     1261 aagccggaga atagataggt cggcggaatc tatttctcgg caaaagcgga ccaaacccgg
     1321 caagccgcca caagcagcag atctgatcat cgctacatcg accggtgcgg cacctgtccg
     1381 ctggccgatc aattgcgcgc aatgaacgca caaaccagcg aacagaggga atcatcggcc
     1441 gcatctttgg cgaaggagca aaatcatggc tcgtcacatc aataccagcg aaatcgaaat
     1501 ggaacgcgcg ccgattgccg gtacatcact cgaagtgtca cgcgttgcgc taggaacgtg
     1561 ggcgattggc ggttggatgt ggggtggaac cgatgaggcg gaatccatcg cgaccatccg
     1621 cgcagcagtc gagcgcggga tcaacctcat cgataccgcc ccggcctatg gatttggccg
     1681 ttctgaagag atcgtcggca aagcgatcgc cgagggtcat ctccgctcgc aggtgctcat
     1741 tgcgacgaag accggactgg cgtgggaagg tggacgcgta ttccgcaacg ccagccgagc
     1801 gcgaatcctg caagaggtcg aagattcgct gcgccggcta cgaaccgatc acatcgacgt
     1861 ctaccaagtc cactggcccg acccgctggt gacgatcgag gaaaccgcgg aggccatgca
     1921 gagcctgttc aagcagggca aaatccgcgc catcggtgtc agcaatttct cggtgcggca
     1981 gatggagcgc ttccgccgcg tcgcgccgct gcatgtgctg cagccgccct ataatgtgtt
     2041 cgagcgcggc atcgaggccg acacactgcc ctattgccgg aagaacaaca tcgcgacctt
     2101 cggctacggc gcactctgcc gcggcctttt gtccggacgc atcgggttgg acagcagatt
     2161 ccaaggcgac gatcttcgcc gcaccgatcc aaaatttcag cagccgcgct tcggccagta
     2221 cctcgctgca gtggaacggc tcgatcagct cgcgcagcgg cgcttccgca agagggtcat
     2281 ccatctggcg gttcgctggg cgctcgatca aggaatcaca accgcattgt ggggcgcgcg
     2341 ccacgccgac cagctacaac cggtcgacga ggtgagcggc tggaaactcg atgccgccgc
     2401 caaggcggaa atcgaccgca tcctgaagac tacgatcaaa gatccggtcg gccctgaatt
     2461 catggccccg ccggcgagca acgttgcggc gtgaacgcag cgctgaaacc atacgttcaa
     2521 cttttttgca gcgcgggccc gtcttgcggc gggccgcaaa cccgagaatt ccaagctgat
     2581 ctaatacctt gatatgattc ttgtgatgag cccggcgaca tatcttcctt gcatgcaagc
     2641 cttctcaacg gccgggaatt cctctccatt gcgtctccat aggatcagca attgcagacc
     2701 cccccgctca tctcgatcat tgatgacgat gaatcgatgc gctgcgcggt gaagagcctc
     2761 gtgacgtcgc tcggactcaa tgcttacacg tttgcatcgg cggaagaatt cctgcgatcg
     2821 ccgcatgtcg agcatacgtc atgcctaatc tccgatttgc aaatgccggg gctcagtggc
     2881 gtcgaactgc agaatacgct gttggcgcag gatcgccgca ttcccattat tttcatgacc
     2941 gcgtttcccg aggagcggat gcgcacccgc gcgatgaagg atggggcctt gggctttctg
     3001 agcaagccgt tcgaaagtca aatgctgata aatctcatcg agaaggcgat cgggatcagt
     3061 cggaaagaat gaacttgcgc gcgctcgctc agaacatctc cagcgactcg caagcagtcg
     3121 tgctgccgga caacgtcgct ccatagccga aaggctcggc gtcgcttccg aagtcccgca
     3181 tcctcatcga atccgtctca gctttttccg gtgacgcact cccgtcgcac ccgccaacga
     3241 ctcaacggac attcgcgacc gggacacttt cgcccaagcc atggcaaggc cgcctcatgc
     3301 cccaaaccaa ggtataggtc gcgcaaacca atgagatacg cgtctcaacc tacgtacgat
     3361 ggagctattc accctgctcc acttaggttt acggcagccg gggaggacac ggctgctggt
     3421 tctacaaccg agctgccaag atgcaaggtg caacgtcatg ctgagccaat cccttcgcac
     3481 caattctgct cgcaccaatt ctgctccagg tcctcgcttt cacaacggcg tgccttacgg
     3541 ccgcctcggg tcgttcgtgc tgcgcaacgc gatgttgcgc tccgtggacg agcacgcgcg
     3601 cttgaacgaa ggcgagctgc gcccctatct gccgagcaag ctctcccatt ttcattccgg
     3661 cgccgtcatg gatcaggcgg tcggaatctt tccctccgat gcagtcgcac gccaggggct
     3721 tgcatggggc ggcatggcgg tggaggtcgt gcaagcgatc acacatgaga gggtcgagtt
     3781 ccgctttcgc ggatttcaac acttgctcgt cgtacatgaa gagggcgttc gtgcaagcgg
     3841 cgaaacgctg atcgagggtt taccccgttc cacattgagg gacgtcaagc gcaagctcgc
     3901 ctttgtgccg gctggtcacg aataccgcga gacgcaggag ccccgcaccc gcagccgcgt
     3961 ggtgtatatc tatttcgatc ccgccaaaat gcgccgcagt ccgggaccgg tcctaaccga
     4021 gaggccgttg gcgccacgct tgttcttcga agacaaggcg ttgtgggaaa cagcggtcaa
     4081 actcggcaga ttgatcgaga gcggcactga agaccgacat tatcgcgagg cgcttggaat
     4141 cgtattggcg cacgagctcg tgcaccgcaa cgacggcgcg cagcgcattg agccgccagc
     4201 acgcggtgga ctggccggct ggcagcagcg gatcacggcg gcgtatatcg aagaacatct
     4261 cgccgagccc atagcgctcg ccaccttggc gcgctccgtt cggctgagcg cgtatcattt
     4321 ctgccgagcg ttcaagcgat cgttcggcgt gccgccccat cgctaccaca acgcccgtcg
     4381 catcgaacac gccaaaacgc tcttggccga gcccacctgc tcggtgaccg ggatcgcgct
     4441 caagatcggc tttagcgaaa ccagttcgtt caccgccgcc tttcgcaagg cgaccgggac
     4501 gacgccgacc gcataccgcc gcagccttgc atcactcgcg aggccgtcat gacccgccgg
     4561 ctgatcacca tagcgactgc gatcttgttg gggtcgagct gtgcttatgc gcaaggcggc
     4621 ggaatgacgc ccttgatgcc gagcgtgggc atgggcatga cttcgccctt gggcatgagc
     4681 agctccggga cggcgattgc gccaaccgga attcccttcg gagccgcccc gctggcaacg
     4741 cctggcgtca gttcgggctc atccaacgga ctcggcttat ccacggcgtg ttccagtccc
     4801 ggcacggcag gtgcgatgac gaccacatcg acctcgaccg cgccgttcga caacagcggc
     4861 gtgggactca tgacgggcgc gacgccgccg ggtccaatcg cgacgagctg cgggccggct
     4921 tctgcgacag gcagcatcac gtccttccag tcgagcactt cgacgcctgc gagctcgcaa
     4981 ctgggcgttc ccaccattcc gatggggtcg accgaactga gcaatccggg tctgagccca
     5041 gcgccgtgtc gggcaacggg cgtcactgca cagtcttccg gtgcctgctg atgcgcaagc
     5101 gcccagtggg gagcatccga tgcgaagcaa ggtgatcatt tccacgattt cggcagtcgt
     5161 cgtcgccgcg atcgtcggcg cgttagtcgt gacgcatcgg tttccatcca aggcggcgtc
     5221 ggcgagcacc ccggcagtcc caaccgctcc cgtcgttgcg ggcgccgtca ccagccaaga
     5281 cgtgcctatc tatctgcaag gggtcggcac ggtgatcgcc tacaacacag tgatcgtgcg
     5341 cagccaaatc caggggcaga tcacaaaagt caatttcacc gaagggcagg ccgtaaagac
     5401 cggcgacctg cttgccgtga tcgacccgcg cccttatcag gcgcagctcg atcaggcgac
     5461 ggcgaaccgc agccgcgatc aggcgcagct aaccaacgcc gaatccaatc tcaaacgcta
     5521 taccaccttg ggaaaccaag gctgggccac gccgcaattg gtcgagaccc agactgcgca
     5581 ggtcgcgcaa ctcaacgccg cgatcaaggc cgacgaggcg gcgatcgaat gggcgcagac
     5641 gcagctcagc tatacgcagc tcacctcgcc catcgatggc gtcaccggca tacggcaggt
     5701 cgacatcggc aacatcattc accccaccga cccgaatggc ctggtcgtgg tgacgcaggt
     5761 cgagccgatc tcggtgatct tcacgctgcc ggagacgagc ctgccgcaga ttcaacagca
     5821 attagccgat gggtcgctca aagtggaggc ttacagtcag gacaacacga tgaagctcga
     5881 tgaaggcagc ctcgatttca tcgacaacga aatcgtgcag accaccggca gtgtgcggtt
     5941 tcgagccaac ttccccaaca aagagcatca cctgtggccg ggagaactcg tcaatgcgtg
     6001 gctcctgctc gacacgcgcc atgacggcct gacagtgtcc gcgtcggcgg tgcagcaggg
     6061 gccgcaaggg ccgtacgtct acgtgatcaa cgcggacaac acggtcgccg tgcggccggt
     6121 caaagtcgcc caggtgagcg gtgggcgggc gctcatcgat tccggcctcg cggcgggcga
     6181 gcaggtggtc gtggacggcc aatacaagct gcagcctggc atccacgtca caatcttgca
     6241 cggcaaggcg gcagaggagg ctgcggcgca agaagcgcag cagacaccga tcccatgaat
     6301 atctccgccc cattcatcgc acgcccgatc gcgaccgcgc tgctgatggt cggcctgttg
     6361 ctaggcggcc tcgccgccta tccactgctg ccgatcgcgg cgctgccaaa cgtgaactat
     6421 ccgaccttgc agatcacggc ccagctgccc ggtgccgatc cgcagaccat ggcgtcttcg
     6481 gtggcaacgc cgctcgagac gcagttcggg caaatcccgg gcctcacgca gatgacctcc
     6541 gcgagcgcgc tgggctacac gcagatcacg ctgcagttcg atttgagccg gcaaatcgat
     6601 ggggccgtgg cggatgcgct atccgctatc aacgcggcca gttcctatct gccgcccaac
     6661 ctgccgtacc ccccaacgat caacaaagtg aacccagccg acacgccgat cctcgtgcta
     6721 gcactcacgt cggacacgct gccactcacg accgtggatg cctacgccga aaacattttg
     6781 ttgcaaaaga tctcgcaaat atcaggagtc ggcttggtcg gaatcgccgg acggcaaaaa
     6841 ccggcaatcc gcatccaggt cgatcctcag gccctggcgg cgcgcgggct gagcctcgaa
     6901 gacgtccgca gtgtgatcgg cgtggacaac gtcgatcttc ccaagggcac gctcaacagc
     6961 ccgcgccaaa cctacatcct caacaccaat gaccaactgt tcaaaccgga agcctatgcc
     7021 gacctgatca tcgcctaccg caacggttcg ccggtgcggg tcaaggatgt gggaaaagcg
     7081 atcagcggcc cggaaaacaa cctgatcgcc gcctggttca accagaaacg ggccatcatc
     7141 cttccgatcc agcgctcgcc gggggcgaac gtcatcgcca cggtcaaccg tatcaaagcc
     7201 atgatgccgg tgctgcaggc ttccatcccg tcgggcatca agatcgacac cgtgtccgat
     7261 cgaaccgaga ccatccgcgc ctcggtcacc gacgtgcaat tcacgctggt cctttcggtc
     7321 gcgttggtgg tgatggtcat ctttctcttc ctgcgcaacg tctgggcaac gatcattccc
     7381 gcggtcaccg ttccgctgtc gttgatcggc accgtcgctg tgctctacga gctgggctac
     7441 agcctcgaca atctttcctt gatggccctg acgatcgcga ccggctttgt cgtcgacgat
     7501 gccgtggtcg tgatcgagaa taccgtgcgc catctggaag aaggtctgtc gccgatgcag
     7561 gccgctatca agagcgccgg cgagatcggc ttcaccatca tatcgatcac gctctcgctc
     7621 attgccgtgt tcattccgct tttcctcatg agcggttatg tcggcgcgct attccgtgaa
     7681 ttcgccgtcg ccgtcagcgt ctcccttctc ctctcgctcg tgatcgcgtt gacgctgacg
     7741 ccgatgatgt gctcgcgcct gctcaagccg gagagcaagg agcatggccg catctaccgt
     7801 ctgttcgaac gcggcttcga tgcccttctc ggcgcctatg aggccggcct gaaggtcgtg
     7861 ctccgccatc gcttaatcac gctgatggtg atgctcggta ccctggcgct caccggatac
     7921 ctgtttattg tgatccccaa aggcttcttt ccgcagcagg acaccggatt gatcatcggc
     7981 ttttccgagg ccgcgcagga catttcctat caggcgatgg ccgagcggac gcaggcgttg
     8041 atcaaggctg tgctggagga tccggcagtc gcttcggtcg gcaccggcat cggcgccggc
     8101 ggcggcaact acacgaccaa caccggccgc atgttcatct cgctcaagcc caagaaccag
     8161 cgtgacgcca gcgcggatca agtgatcctg cggttgcagc aaaggctcgc caagatccag
     8221 ggcattgcgc tttatatgca ggcggcacag gacatcacga tcggcggccg gctgaacaag
     8281 acgcagtacc agtacacgct ggcggactcc gatccgggcg agctcaatca ttgggctccg
     8341 ctgtttctcg agaagatcag ggcgctgcca ggcatcaccg gcgtcaccac cgaccagttg
     8401 aatgcgggcc cgctgctcga catcacgatc aatcgcgatg tggcctcgag ctacggcatc
     8461 actccctcaa ccatcgacaa catcctcaat gatgcctacg gccagcgcat cgtttcgacg
     8521 atgtacacgc agatgagtca gtaccacgtc gtgatggagg tcccgcccaa gtatcagtac
     8581 gacccggaag cgctcggcca gctgtacgtc agttcgtcga gcggccagca ggtgccgctg
     8641 cgcaccctca tcactagcgt cgtcaaagtg tcgccgctgg tggtcaatca tcagggccag
     8701 tttccgtcgg tgacaatctc gtacaaccta tttccgggga cgtccctcgg cgaagccgtc
     8761 gcctccattc aacaggtcga gaaggaactg ggcaaacctc tgtcgttgca aaccagcttc
     8821 caaggaaacg ggcaagccta tctgcaatcg ctgtccagca cgccgatcct aatcctcgct
     8881 gcgctggtcg tcatttatct catcctcggc attctctacg agagcacggt ccacccgatc
     8941 accattctct cgaccctgcc atccgccggc atcggggcgc tcttgctatt gatggcctcc
     9001 ggcttcgata tgagcgtgat tgcgatcgtt ggcatcctgt tgcttatcgg aatcgtcaag
     9061 aagaacggca tcatgcttgt cgacttcgcc attcacggcg agcgcaacga gggcctggga
     9121 ccggaagagg cgatctacca ggcttgcatc aagcgcttcc gccctatcct catgacgacg
     9181 atggcggcga tgcttggggc cgtcccgctc atgctcggca cgggaaccgg ttccgaaata
     9241 cgcgagccgc tcggctacgc catcgtcggc ggattggcat tgagccaatt cctcacgctc
     9301 tacaccacac cagtcgtctt cctttatctc gatcgtttga ctagacgaca cccccgcagg
     9361 cccgcagcga gcgacggcag cgcggcaccc aagccgcacc cggccgttcc gagggaggcg
     9421 caatgatggg acgcatacaa tgacatcgcc gttcttacga ctgagaagct aaaatcatga
     9481 tgatgaccag acggactcta tccgccgccc tggtagccgg tgcggccgct tcgctaatct
     9541 ccacgcgtgg catggccgca gactccccac cgacgcaggc acgcaacgtc gtgctcgtgc
     9601 acgggctgtt tgccgatgga tcgtgctggt ccgaaatgat tgcgcgattg cagctggctg
     9661 ggctcaatgt cacggccgtg caaaacccac tgacgacgct acctgaagca gtcgcttcga
     9721 accgccggca acggccggca tcgttttcga cggcgatgaa ggacgtctca ccgaggaagc
     9781 gttcctgcgc gacttcgcgg gcg
//
LOCUS       DATLJT010000364         7718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1858287,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000364 DATLJT010000000
VERSION     DATLJT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858287"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VM910_08455"
     CDS             complement(<1..689)
                     /locus_tag="VM910_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62602.1"
                     /translation="MGASSGPRPRRSAFAFLVPVRVIEMKQPSWRSVLPIHPAAELFP
                     RMPPDERRVLGEDIRKHGLREPVVLQRQYRRRADGSIANEHDYKLVLLDGISRLDSME
                     EADFPLVRDGKLDKTLGHAALGLKPLTDGAYAELDPGIDPYDFVVSRNILRRHLNAKQ
                     RRELIAKLLKADASKSDRQIGEMIKADHKTVGAVRAEKEATGEISPVEKRVGKDRKAR
                     KQPVKKATTAKS"
     gene            complement(851..1531)
                     /locus_tag="VM910_08460"
     CDS             complement(851..1531)
                     /locus_tag="VM910_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62603.1"
                     /translation="MNGAQNLTEPVAAQAGPDASPRNRRDWTYFDERLSELRKNDVEN
                     IIARGRILIEANDELERGSYEATVKRHFDLSYARKLRIIAAHPVISNRAHVHALPPSV
                     FTLYELTKLPAALLRETLNDGSINAKLERKDVARWRKGEGGKVAVDGKTVERKSLREQ
                     LKAAKVEIETLEAKLKDAGGGYFDLALDSAEHIGQQLAEHMSEARFDTAVKAAKARFK
                     AKRQKPAG"
     gene            complement(1598..1885)
                     /locus_tag="VM910_08465"
     CDS             complement(1598..1885)
                     /locus_tag="VM910_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62604.1"
                     /translation="MDIDEQPLDPRCREAHPERITIGGETFVRNDILAREQGESERSL
                     NRGDKRGAPFRFFGGVKYRPQRLHAEFIMNTVQVRKPQPPKRSQKKIRRRT"
     gene            complement(1969..2388)
                     /locus_tag="VM910_08470"
     CDS             complement(1969..2388)
                     /locus_tag="VM910_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62605.1"
                     /translation="MSKRKLRDPVTLEIALEMLEVLRDARSFARDLERRGLSPDIEWM
                     EATLAKFWAPEQDDTAWNAFDHIEQRLRRTRTVLEQLAAAQPRQQAVRIKREVDALTD
                     EFKRSGARQPRKLAWEKVAKRERRSVAALKRWANRNC"
     gene            complement(2385..3590)
                     /locus_tag="VM910_08475"
     CDS             complement(2385..3590)
                     /locus_tag="VM910_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018516872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI62606.1"
                     /translation="MAERKRLTDAIVRRLPLPQQGKHITIDADVPGFGARVTANGARS
                     FILRYTTRAGRERTFTIGGASVWRTTDARDKARELRREIEDGGDPLGDIEDERAAPTV
                     VDLIERFRSEHLPKKRPRTASEYGYLLQLHIEPHFGQHTKVADVRFEDVDALHRKITK
                     SGSPYAANRTIALLSKMFGLAIKWRMRETNPVKGIERNREYHRRRYLSGKELIRLTEA
                     LAKHPDKKAADAIRLLLLTGARRGEVLGMRWEDVEDGTWSKPPSSTKQKEHHQVPLSA
                     PALQLLSNIRKRQRPRAPFVFPGDGATGHRVELKKNWALITKAASIEGLRIHDLRHSY
                     ASQLVSGGASLPLIGALLGHSNPITTSRYAHLFNDPLRKAAEQVGAVIAAAGKPVKSV
                     LKLKRRGRP"
     gene            4042..4878
                     /locus_tag="VM910_08480"
     CDS             4042..4878
                     /locus_tag="VM910_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62607.1"
                     /translation="MWCKLVLTDVVRTVLRPAQIPSEKFAGVLSAFGRLPEDAQYNLA
                     VPLVNAVETYRLRKRAQKQKTPQAHERRDQLEGISTLASRLLALLGVNEPKSVAGGVR
                     RGSMHPTAASLLRGSAHPKAASLLMWLYRVAVERRPATATASAEERLSTLIMLLSDLV
                     EAAERSAREVSTRPGLGGDRRKGELTPEGDLIQAIIKLYIDFRNRFPSSGPAPAFDKS
                     LRKFVRTVLELAVSCSHYVGPDGKKIEPWYMAAIDRDLAKSTRTTDAAIRGAFQRLHK
                     PK"
     gene            complement(4935..5141)
                     /locus_tag="VM910_08485"
     CDS             complement(4935..5141)
                     /locus_tag="VM910_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62608.1"
                     /translation="MRFKLEKGNATPLGTITLQLASRPLDVARKLAFGEQFFVISKWH
                     CSRPRRSAPDDFRRRESSYDASYG"
     gene            5425..6111
                     /locus_tag="VM910_08490"
     CDS             5425..6111
                     /locus_tag="VM910_08490"
                     /inference="COORDINATES: protein motif:HMM:NF022744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HVI62609.1"
                     /translation="MLDTSSRANFKATAEIDDLDALAEEVRKHLQASAAAAQNFLEHA
                     LAAGDALIRAKERVKHGDWLKWLKSCDLSADTAERYLKLARHRTELNSARVRNLSLSA
                     ALKLVTTKKPVGGPKAKKGGPTTHFDALAWWSSASPEARSHFIDGVGLKPLLAAIPSG
                     WRREAEQAVGPVSPRITTLLQLALSTTSAGEAIAALAAVKRALTANGYDLHDVEIRLN
                     NQNRRLGRAA"
     gene            6122..6487
                     /locus_tag="VM910_08495"
     CDS             6122..6487
                     /locus_tag="VM910_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62610.1"
                     /translation="MTIIDQITDHAHGRKRLPNRRAATSFNVEHNGIRYAATYARFAD
                     GGVGELFISTNKAGSSLDECIRDSGVATSLALQHGCSLDTLRGAVLRHADGSPATALA
                     AAIDEVARILEEPDAAEDE"
     gene            6487..7323
                     /locus_tag="VM910_08500"
     CDS             6487..7323
                     /locus_tag="VM910_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI62611.1"
                     /translation="MQIISADQRLAERRGVKALIVGPTGVGKTSLLRTLDPTRTLFVD
                     IEAGDLSVQDVPVDMFRLDDWGAARDLACRIGGPNPSFPANACYSEAHYQAVGGALDQ
                     IDRYDTIFVDSITAVSRLSFRWAEQQPEAFSERTSKKDTRGAYGLHAREMLLWLYQLQ
                     HARGKHVVFVGILEKVTDEFNATSWQPQMEGARTGRELPGIVDQIVTMQWIDFGDGAP
                     TRAFVCTSPNPWAYPAKDRAGRLEQIEEPHLGKLITKLISPGQRKPFIVSPKQSLTQA
                     AE"
     gene            7333..>7718
                     /locus_tag="VM910_08505"
     CDS             7333..>7718
                     /locus_tag="VM910_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62612.1"
                     /translation="MAGYDFNDADTQRSFDVIPDGTIATVRMTVRPGSAGEGGWLRRS
                     KDGGSEALDCEFVVLDGPFAKRKFWTLLTIAGTTPGHGEAANISAGKLRAMLESARGI
                     KPDDTSDAAKQARRIGSYGDLNGLSF"
BASE COUNT         1401 a         2394 c         2381 g         1542 t
ORIGIN      
        1 gacttcgctg tcgttgcctt tttcaccggc tgcttacgcg ccttgcggtc cttgccaacg
       61 cgcttctcaa ctggggagat ttccccagtt gcttccttct ccgcacggac cgccccgacc
      121 gtcttatggt cggccttgat catctcgccg atctgccggt cggacttgct cgcatcggcc
      181 ttgagcagct tggcgatcaa ctcgcgccgc tgtttggcat tcagatgccg ccggaggata
      241 ttgcgtgaga cgacgaagtc atacgggtcg ataccaggat caagttcggc gtaagcgccg
      301 tcggtgagcg gcttcagtcc tagcgcggca tgtccgagag ttttgtcgag cttgccatcg
      361 cgcacgagcg ggaagtcggc ttcctccatt gaatcgagcc gactgattcc gtcgagcagc
      421 acgagtttat aatcgtgctc gttcgcgata ctgccgtcgg cacggcgtcg atactgtctc
      481 tgcagcacga ccggctcgcg taagccatgt ttgcgaatgt cctcgccgag cacccgccgt
      541 tcgtcgggcg gcatccgtgg gaaaagctca gcggcgggat gaatggggag gacggaacgc
      601 caggatggtt gcttcatctc aatcacccgt acaggaacca aaaatgcgaa cgccgagcgg
      661 cgcggccggg ggccgctgct ggctcccatc cttcgccgcc cgcgcggcga cgcgcgcgaa
      721 tcacggcata gcaagcgttg atgctgttct cgaagtcagc ggcggcgtcg tacacttcga
      781 ccgcgtaatc cggccagcga tcgcgatgct cttcgcaccg ccagatgggc tttacaggtc
      841 tgaacactct tcaccccgct ggcttctgcc gcttggcttt gaagcgcgcc ttagccgctt
      901 tcactgccgt atcaaaccgc gcctcgctca tgtgctcggc gagttgctgt ccgatgtgct
      961 cggcgctgtc gagcgcgagg tcgaaatatc cgccgccagc atccttgagt ttggcctcca
     1021 gcgtctcgat ctccaccttg gccgccttga gttgctccct cagtgacttg cgctcaactg
     1081 tcttaccgtc gacggcgact tttcctccct cacccttgcg ccagcgggcg acgtctttgc
     1141 gttccagttt ggcattgatg ctgccgtcgt tgagcgtctc gcgcaggagc gcggcgggca
     1201 atttggtcaa ttcgtagagc gtgaacacgg acggcggcaa agcgtgcacg tgtgcacggt
     1261 ttgaaatgac cgggtgtgca gcaatgattc gcagcttgcg cgcgtagctg agatcgaagt
     1321 ggcgcttgac cgtcgcttcg taggagccgc gctcaagctc atcattcgcc tcgatcagaa
     1381 tccgtccgcg cgcgatgatg ttctcgacgt cattcttgcg tagctcgctg agccgctcgt
     1441 cgaaatacgt ccaatcgcgc cgattcctcg gcgacgcatc ggggcctgct tgcgctgcga
     1501 ccggctcggt gagattctgc gctccgttca ttcgtgtctc cagcacgaaa gcacatttga
     1561 ccccgggcga cgtgggcaac gacaccgggc tcgcttctca ggtccgtctt cggattttct
     1621 tttgcgatcg cttgggcggc tgtggcttgc gcacttgaac agtgttcatg atgaattcgg
     1681 catgcagccg ttgcggccga tatttcacgc cgccgaaaaa tcggaacggt gcgccgcgct
     1741 tgtcgccgcg attgagcgag cgctcgctct cgccctgctc acgcgccaga atgtcgtttc
     1801 ttacgaaggt ttcgccgccg atcgtaatgc gctcgggatg cgcctcgcgg cagcgaggat
     1861 cgagcggctg ctcgtcaatg tccatttttc gtcacctctt tgcgtcaaga gatgacgacc
     1921 tatttagaac atgaaattgg gtttgtcgcg ggacattttg tgtcccactc aacagttgcg
     1981 gttcgcccat cttttgagcg ccgcgacgct gcgcctctct cgcttggcaa ctttttccca
     2041 agccagcttg cgcggttggc gagcgccgct gcgcttgaac tcgtcagtga gcgcatcaac
     2101 ctcgcgcttg atgcgcaccg cttgctgtct aggctgcgcc gccgcgagtt gctctagaac
     2161 cgtccttgtc cgccgaagac gctgctcgat gtggtcgaac gcgttccacg cggtgtcgtc
     2221 ctgctccggt gcccagaact ttgcgagcgt ggcctccatc cactcgatat cgggggaaag
     2281 gccgcgcctt tccaagtccc gcgcgaagga gcgagcatca cgcagcacct caagcatctc
     2341 cagcgcgatt tcgagcgtga ccggatcgcg taatttgcgc ttgctcatgg ccgcccccgc
     2401 cgcttgagct tgagcaccga cttaaccggc ttgccggcgg cggcgatcac cgcgccgacc
     2461 tgctccgcgg ccttgcgcaa cgggtcgttg aacaagtgcg cgtagcggct cgtggtgatc
     2521 gggttgctat ggcctaacag cgcgccgatc agcggcaagc tcgcgccgcc gctcacgagt
     2581 tggctggcgt aggaatggcg caggtcgtgg attcgtagcc cttcgatgct cgcagccttg
     2641 gtgatcagcg cccaattttt ctttagctcg acgcgatgcc ccgtcgcccc atcaccggga
     2701 aacacgaatg gcgcgcgcgg acgttggcgt ttgcggatgt tcgataagag ttgcagcgca
     2761 ggcgcggaga gcgggacctg atggtgctct ttctgcttgg tgctgctcgg cggcttcgac
     2821 caagtgccgt cctcgacatc ctcccaccgc atgccgagaa cttcgccgcg ccttgcgccg
     2881 gtcaacagca gcaaccggat cgcgtcggct gccttcttgt cgggatgctt ggccagcgct
     2941 tcggtcagcc gtatcagctc tttgccgctg agatatcgcc gccggtgata ttccctgttg
     3001 cgctcgatcc ccttgaccgg attggtctcg cgcatgcgcc acttgatggc gagcccgaac
     3061 atcttggaca gcagcgcgat ggtgcggttg gcggcgtagg gcgagccgct cttggtgatc
     3121 ttgcggtgga gggcgtcgac atcctcgaag cggacatcgg ccaccttggt gtgttggccg
     3181 aaatgcggct cgatgtgcag ttgcagcagg tagccgtatt cgctggcggt gcgggggcgc
     3241 ttctttggca ggtgctcaga ccggaaccgc tcgatcaggt cgaccacggt gggcgcggcg
     3301 cgctcgtcct cgatatcacc gagggggtcg ccgccgtcct cgatctcgcg gcgtagctca
     3361 cgcgccttgt cgcgagcgtc cgtggtgcgc cacaccgaag cgccgccgat ggtgaacgtg
     3421 cgctcccgtc ccgccctggt cgtgtatctg aggatgaagg accgcgcgcc gttggcggtg
     3481 acgcgggcgc cgaagccggg aacgtcggca tcgatggtga tgtgcttgcc ctgctgggga
     3541 agtggaagcc gccgcacgat ggcgtcggtg aggcgttttc gctcagccat gaaaatctct
     3601 aagcccgtcc tatgcccggc aatctgacga catgccgtgt cctgggatgt aacggagatg
     3661 tcccgacatt gcaatgagaa atgctgatgt gacgggcgtt attcgtgcgt ctcgtggaga
     3721 aatgctggag gtgcaagtgc ttgcgaaatg cgcccggatc aaactacgaa tctgggggtc
     3781 aggatttcga atctcttcgg gcgcgccaga ttaccccaag atctcagaat tatagcttag
     3841 caggttggcc agagattttt tgctatttct agagcggacc tccagcggac ccagggagcc
     3901 tgtttcgggc gcggaaagcg cctcggcgtc cacttgttaa ttgttggtga ctggccctgg
     3961 cgcgctttgg cgacgcatgg cgatccgtgc cagtatcgcg ctccatgcaa tcatcgcgcg
     4021 acggagggct ggtcttaacg gatgtggtgc aagctggtct taacggatgt ggtgcgaaca
     4081 gtactgcggc ccgcgcaaat cccctccgag aagtttgccg gggtgctatc agcgttcgga
     4141 cggttgcccg aagatgcgca atacaacctc gccgtccccc tcgttaacgc ggtggaaacc
     4201 taccgtctca gaaagagagc ccaaaagcag aaaaccccgc aggcacacga gcgacgtgat
     4261 caactagaag gcatcagtac cttagctagc cgcctccttg cgctacttgg cgtcaacgag
     4321 ccgaagtcag ttgctggcgg cgttcggcgg gggtcgatgc atccgacggc cgcgtctctt
     4381 ttgcgggggt cggcgcatcc gaaggccgcg tctcttttga tgtggcttta tcgtgtcgcc
     4441 gtcgagcggc ggcccgcaac agccaccgcc agtgctgaag agcggctgag caccctcatt
     4501 atgctgctat ccgatcttgt agaagccgca gagcgatccg cacgtgaggt aagcacgcga
     4561 ccgggcttgg gtggggatcg gcgcaaagga gaactcaccc cggaaggtga cttaatccaa
     4621 gccattatca agctgtacat cgattttcgt aaccgttttc cgagcagcgg tccagcgccg
     4681 gccttcgaca agtcgttgcg aaaattcgtg cgcacggtgc tggaacttgc cgtcagctgc
     4741 tctcattacg ttggcccgga cggaaagaag attgagccat ggtacatggc cgcgatcgat
     4801 cgtgatctcg caaaatcgac ccgaaccacg gatgcggcca ttcgcggggc atttcaacgc
     4861 ttgcacaaac caaagtaaaa tcgcgactga tttaaacagc tgtggccgct ttaaacagct
     4921 gttgccgcac ttcctcagcc atagctcgcg tcgtaagaag actcgcgacg gcggaaatcg
     4981 tcgggcgccg accttcgagg acgcgagcaa tgccatttgc tgatgacgaa gaactgctca
     5041 ccgaacgcga gcttgcgcgc tacatcaagc ggtcgcgacg ccagctgcag cgtgatcgtg
     5101 ccgagcggcg tggcattccc tttctccagt ttgaaacgca agtccgatac cgccttgggg
     5161 acgtgcggac gtacgtcgcg cgccacgtag ttgggtcaaa cggcgagcag ggagcggcca
     5221 tcgttttgtc ccaagcgacg cggtcatctc gcaccgcgaa gcaatctgct taggaaatct
     5281 gtgaatttcg gcgcaggtga tcgccgccga cctgcgttga gatttccaat aaatcggcgg
     5341 caacaaagcc ccggcccgcc gaacgaggcg gcgggccgga tccctcggca gggatttgcg
     5401 ctcaagtaag gagaagaacg cgaaatgcta gataccagtt ctcgggctaa tttcaaggcc
     5461 accgccgaga tcgacgatct cgacgctctc gccgaagaag tcaggaagca cctgcaagcg
     5521 agcgcggcgg ccgctcaaaa cttccttgag catgcactag ctgccggtga cgcgctgatc
     5581 cgcgccaagg agcgggtcaa gcatggcgac tggctaaagt ggctgaagtc gtgcgatctg
     5641 agcgcggaca cggccgaacg gtacttgaaa ctggctcggc accgcaccga gctaaattcc
     5701 gcacgcgtgc ggaatttgag tttgagcgcc gccctaaaac tggttaccac gaaaaagccg
     5761 gtgggcgggc cgaaggcaaa aaagggcggg cccaccactc acttcgatgc gctcgcgtgg
     5821 tggtcaagcg cttccccaga agctcgatcc catttcattg acggtgtggg actcaagccg
     5881 ctgcttgcgg cgatcccgtc aggctggcgg cgggaagcgg agcaggcggt cggtccagtt
     5941 tctcctcgca tcacaacgct gctgcagctc gcgctgtcaa cgactagcgc cggcgaggcg
     6001 atcgctgccc tcgccgccgt gaagcgggcc ctcaccgcca acgggtacga cctccacgac
     6061 gtcgagatcc gcctcaacaa ccaaaatcgc aggctcggtc gcgccgcttg agttcaccgt
     6121 tatgaccatc atcgatcaga tcaccgacca cgcacacggt cgcaagaggt tgcccaacag
     6181 gcgcgcggca acgagcttca acgttgagca caacggcatc cgctacgccg cgacctacgc
     6241 gcggttcgcc gatggcggcg ttggtgagct cttcatttca actaacaagg caggatcttc
     6301 tctcgacgag tgcattcggg actcaggtgt cgccacgtcc ttggctttgc agcacggctg
     6361 ttcgctcgac acgttgcgcg gcgcggtgct gcgtcatgcc gacggcagcc ccgcgacggc
     6421 gctcgccgcc gcgattgatg aggtcgctcg catcttggaa gaacccgacg ccgcggagga
     6481 cgagtaatgc aaattatcag tgcagaccaa aggctcgccg agcggcgcgg cgtaaaggct
     6541 ttgatcgttg gcccgaccgg cgtcggcaag acgagcctgc ttcgcacgct cgatccgacg
     6601 cgcacgctgt tcgttgacat cgaggccggc gacctcagcg tgcaggacgt gccggtcgat
     6661 atgttcaggt tggacgactg gggggccgcg cgtgatctcg catgtcggat tggtggacct
     6721 aatccatcgt tcccggcgaa cgcgtgctac tccgaggcac actaccaggc ggtcggcggt
     6781 gcgctcgacc agatcgatcg ctacgacacc atcttcgtgg attcaatcac ggccgtgtcg
     6841 cggttgtcgt tccggtgggc cgaacagcag ccggaagcgt tcagcgagcg caccagtaag
     6901 aaagacacgc gcggcgccta cgggctgcac gcgcgcgaga tgttgctatg gctataccaa
     6961 ctgcagcacg cgcgcggcaa gcatgtcgtc tttgtcggca tccttgaaaa ggttaccgac
     7021 gagttcaacg cgacgagctg gcagccgcag atggaaggcg cgcgcaccgg ccgcgagctg
     7081 cccggcatcg tcgaccagat cgtcacgatg cagtggatcg acttcggcga cggcgcgcca
     7141 acgcgcgcat tcgtgtgcac gtcgccgaac ccctgggcct accccgccaa agatcgcgct
     7201 ggccggctcg agcagattga ggagccacac ctcggcaagc tcatcacgaa gctgatcagc
     7261 cccggccagc gcaagccgtt catcgtttca cccaagcagt cgctcaccca agcagcagaa
     7321 tagggggcca agatggctgg ctacgacttc aacgacgcgg acacccagcg cagtttcgat
     7381 gtgattccgg acggcacgat cgccaccgtg cgcatgaccg tccgccccgg tagtgccggc
     7441 gagggcggct ggttgcgccg ttccaaggac gggggcagcg aagcgctcga ttgcgaattc
     7501 gtcgtgctcg acggcccctt cgccaagcgc aagttctgga cgctcctcac cattgccggc
     7561 accacacccg gtcatggcga agcggcgaat atttcggccg ggaaactgcg cgccatgctg
     7621 gaatccgcgc gcggcatcaa gcccgacgac acgagcgacg ccgcaaagca ggcgcgccgc
     7681 atcgggtcgt acggcgacct caacggcctc agtttcat
//
LOCUS       DATLJT010000365         1892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3696741,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000365 DATLJT010000000
VERSION     DATLJT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696741"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VM910_08510"
     CDS             complement(<1..219)
                     /locus_tag="VM910_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62613.1"
                     /translation="MMLTRRSLLAVSAAWLFGAADAAAQDWPQRSVRVIVPFGPGGGA
                     DIIGRIVAQSLQEKLGQPVVIENRPGAGG"
     gene            complement(521..1480)
                     /locus_tag="VM910_08515"
     CDS             complement(521..1480)
                     /locus_tag="VM910_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI62614.1"
                     /translation="MSAHLLGDRKTGTALPNREAMIRIPGGSFRMGSNDFYREERPVR
                     GETVQGFWIDPYPVTNADFRKFVSATGYVTTCERPPDPAIYPDADPSLLVPGSSVFRK
                     PRGPVDLRDNRAWWEYVPGADWRHPQGPGSNIEGRDDHPVVHVTYEDACAYAAWAGKE
                     LPVESEWEFAARGGLDGATYAWGEASAPHGRAMANTWQGRFPWENLKLDGYEGTSPVD
                     AFPANGYGLYDMIGNVWDWTASRFTPHGGEISGKSCCVPSAPGGARTARRVVKGGSHL
                     CAPNYCLRYRPAARQAEAVDTSTCHIGFRCVVRTSENRVALPA"
     gene            complement(1500..>1892)
                     /locus_tag="VM910_08520"
     CDS             complement(1500..>1892)
                     /locus_tag="VM910_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVI62615.1"
                     /translation="FAGCEPPRDRIIDGVDQSPFFLGKQQASNRDACMVWLNEELHAI
                     KWKDFKINFKRQQHFHDPELPLGFARITHLMEDPKEREPVNQRYVRWWVMQHAHRVVR
                     EFEESKKKEELIPTGSPLDFVPRPYART"
BASE COUNT          317 a          608 c          615 g          352 t
ORIGIN      
        1 gccgccggcg cccggccggt tctcgatcac caccggctgg ccgagcttct cctgcagcga
       61 ctgcgcgacg atgcggccga tgatgtcggc gccgccgccg ggtccgaacg ggacgatcac
      121 gcgcacggac cgctgcggcc agtcctgcgc cgcggcgtcg gccgcgccaa aaagccatgc
      181 ggcggagacg gcgagcaggc tgcgtcgcgt gagcatcatt ttcctccctc ggcccgcggc
      241 tgcgagccct cggacgcggc catcattcac cacgtggccg atgtcttagt ctgcaaatgc
      301 gttgtcccgt tgtcgagact tgttcgttgc ccaattcgtg acacttcgcc gcgcaacatg
      361 ccgaagccct agaaccgcag cgcgtaaagg aatcctatgg cgaagcacgc cggctgcggt
      421 ctgcacgtcc gcctcggcag ctcggcagga ccgtgtgacg caatccaatg agcaagcccg
      481 cagaaaattt gtcggctggt tgcgccggtc gattcttgga tcaggcaggt agggcaacgc
      541 gattttcgga cgtccgcacg acgcagcgaa aaccaatgtg gcaagtcgaa gtatcgaccg
      601 cctcggcttg gcgcgctgcc ggccggtagc gcaggcagta gttgggcgcg cacaggtgcg
      661 agccgccttt caccacccgg cgagcggtgc gagcgccgcc gggcgcgctc ggcacacagc
      721 aggattttcc ggatatttcg ccgccgtgcg gggtgaagcg actcgcggtc caatcccaca
      781 cattgccgat catgtcgtag aggccatagc cgtttgcggg aaaggcatcg accggagagg
      841 tgccctcgta gccgtcgagc ttgaggttct cccaggggaa acggccctgc caagtgttgg
      901 ccattgcccg cccgtgcgga gcagacgctt cgccccaggc ataggtcgca ccgtcgagac
      961 cgccgcgggc ggcgaattcc cattcggact cgacgggtag ttcttttccg gcccaggcgg
     1021 cataggcgca agcgtcctcg taagtgacgt ggacgaccgg gtggtcatcc cgaccctcga
     1081 tgttgctgcc gggaccctgc gggtgccgcc aatccgcccc ggggacgtat tcccaccaag
     1141 cccgattgtc ccgcagatcc accgggccgc gcggctttcg aaacacggaa gagccgggca
     1201 ccaagagcga ggggtcggca tccggataaa tagcgggatc gggcggccgc tcgcaggtcg
     1261 tgacgtagcc cgtcgcactg acgaattttc gaaagtcggc gttggtcacg ggataggggt
     1321 cgatccagaa tccttgcact gtctccccgc gtacgggccg ctcttcgcga tagaagtcgt
     1381 ttgagcccat gcggaagctg ccgcctggaa tccggatcat ggcttcccgg ttgggtaacg
     1441 cggtgcctgt tttcctgtcg cccagcaggt gcgcactcat gttgtccgcc aatcgagcct
     1501 caagtccgcg cgtacggcct cggtacgaaa tcgagcggcg atcccgtcgg gatgagctct
     1561 tccttctttt tgctttcctc gaactcccga acgactcggt gcgcgtgctg catcacccac
     1621 cagcgcacgt agcgctggtt cacgggctcg cgttctttcg ggtcctccat gagatgggtg
     1681 atgcgggcaa agccgagcgg cagttccggg tcgtggaagt gctgctggcg cttgaagttg
     1741 atcttgaagt ccttccattt gattgcgtgc agctcttcgt tgagccagac catgcacgcg
     1801 tcgcggttcg aagcttgctg cttgccgaga aagaacggac tctggtcgac gccgtcgatg
     1861 atgcggtcgc gcggcggctc gcagccggcg aa
//
LOCUS       DATLJT010000366         3707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3007764,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000366 DATLJT010000000
VERSION     DATLJT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007764"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VM910_08525"
     CDS             complement(<1..1672)
                     /locus_tag="VM910_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62616.1"
                     /translation="MVDVGQFLGLRTAKRASPSAPAAGQLGIFAGESEPIPQHAVRFS
                     AWQIAALFMTLAAIASIPILLYPWPPLSDYINHLARMHVIASIGSDPNLALFYQVEWQ
                     VIPNLMMDLIVPILQRVMNVYLAGQVYTIMSFVLILSGTLALNRQLYRHWSVLPLIAF
                     PLLYNNVFLVGTMNYLFGIGLSLWALATWVALRERAIVLRLAVSSLFVLALFFCHLFA
                     VGVYGLGLFAFELQRLLALRARKPLGLTSEHPAPRQLPAWVDFVASGIPFLPVVPLLL
                     MSPTWTLRWDFTWEFNGKLNGLLFVIEVYSPSATFFLATIVAIAALFAIRHRALRFHP
                     FGWVLLAVGGVTYLALPRVLFDTYMADQRLPIALAFMIIACAHLDLRDEFVRRGFATV
                     LVLLLAVRAFEVQGVWRELSRGTASFRDSVRQIERGSKVLVAYADPDNGDNARELWLT
                     HAACLAIIERSALVTTAFTVLGKQVMHVRRDYRDRVDTEDGTPPSIRQLLSSAELTDW
                     DDPPYWARWTTDYDYLYILFTDRDYANPDPTRLIPVFAGNRFVLYQING"
     gene            2018..2998
                     /locus_tag="VM910_08530"
     CDS             2018..2998
                     /locus_tag="VM910_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62617.1"
                     /translation="MTFPRRQFLCLAAGAAALAAVPRIGAAQTYPARPVRVIVPFAPG
                     GPTDVFARLMAQKLSEQMGAQFYVENIAGAGGNIGAGRAAQSAADGYTLLVNGANHVV
                     NPALYQRVPYDPTKDFDPVTLAVTAPAVLTVNPTLPVHTVRDLVALIKAHPGKYSYSS
                     PGVGTPPHLVGELFRLSLGLDLVHVPFNGGGPAIASAVAGHTPISFGSTAPAVPLVKD
                     GKLRALAVSTKTRSPALPDVPTMTEAGYPDVAGESWFAVLVPAGTPKEIIALLQRGIA
                     QAIAGPDMKERLATLGYEPVASTPAECAARFRTEIAKWGKVIGEAGIKAQ"
BASE COUNT          769 a         1144 c         1187 g          607 t
ORIGIN      
        1 gcccgttgat ctgatagagc acgaacctgt tgccggcgaa aaccgggatg agccgcgtcg
       61 gatccgggtt ggcgtagtcg cggtccgtga acaagatata gaggtagtca taatccgtgg
      121 tccaccgcgc ccaatagggc gggtcgtccc aatccgtcag ttccgccgac gacagcagct
      181 gccggatcga cggcggcgtt ccgtcttcag tatcgacgcg gtcgcgataa tcgcggcgca
      241 cgtgcatgac ttgcttgccg agcacggtga aggcggtcgt gaccaaggcg gagcgctcga
      301 tgattgccag acaggcggca tgcgtgagcc agagctcacg tgcgttgtcg ccgttgtcgg
      361 gatccgcata cgcgaccagc accttggatc cacgctcgat ttggcggacc gagtcgcgga
      421 acgaggcggt cccgcgcgag agctcgcgcc acacgccctg cacctcgaat gcgcgcacgg
      481 ccagcagcag aacgagcacc gtcgcgaagc cgcggcgcac gaattcgtcg cgcagatcga
      541 gatgcgcgca ggcgatgatc atgaacgcca gtgcaatcgg caagcgctga tccgccatgt
      601 aggtgtcgaa caggacgcga ggcagcgcca aataagtcac gccgccgacc gccagcagca
      661 cccagccgaa cggatggaat cgcaaggcac ggtggcgtat ggcgaagagc gcggcgattg
      721 cgacgatcgt cgccaggaag aacgtcgcgg agggggaata gacctcgatg acgaagagga
      781 gaccgttgag cttgccgttg aactcccagg tgaaatccca gcgcaaggtc caggtcgggc
      841 tcatcagcag cagcggcacc accggaagga acgggattcc gctggcgaca aaatcaaccc
      901 aggcgggcaa ctgccgcggt gccggatgct cggacgtcaa cccgagcggc ttgcgtgcgc
      961 gtagagctag cagcctttgc agttcgaatg cgaacaggcc gagcccgtag accccgacgg
     1021 cgaacagatg acagaagaac agcgccaata cgaacaaact tgatacggca agacgcaaaa
     1081 cgatggcgcg ctcgcgcaac gccacccagg tcgcgagcgc ccagagcgag agcccgatgc
     1141 cgaacaaata gttcatcgtg cccacgagga agacgttgtt gtagagcagg gggaaggcga
     1201 tcagcggcag caccgaccaa tggcgataga gctgacggtt gagcgcgagc gtgcccgaca
     1261 ggatcagcac gaaactcatg atggtgtaga cctgtccggc gagatagacg ttcatgaccc
     1321 gctgcaggat cggaacgatc agatccatca tcaggttcgg aatgacctgc cattcgacct
     1381 ggtaaaaaag cgccagattg gggtcgctgc cgatactggc gatcacgtgc atacgcgcga
     1441 ggtggttgat gtagtcggac agcggtggcc agggataaag caggatcggg atcgaggcga
     1501 tggccgccag cgtcatgaat aacgcggcga tctgccaggc gctgaaacga acggcgtgtt
     1561 gaggaatcgg ctcagactcg ccggcgaaaa tgccgagttg gcctgccgct ggcgccgatg
     1621 ggctcgcccg ctttgccgtg cgcagcccga gaaattgacc aacatcaacc atttcgggct
     1681 acgcgctcag gcgctgcttc accaaagcga tggggagaac aaaggtccgt gcaagtgatc
     1741 gcgtcgccgg gatggcgagc gttcgattga ccaagtcgac gtatcacgtc gacttgccaa
     1801 agtccaccac tgccatgccg cgccagcagt ggggctgcgc gcggaacgcg ttgagcctta
     1861 agaacgtgaa gccagagagc agcatgagcg tcccagtggc ggatgtccga tcgcgcttgg
     1921 ctgccgcgcc ggtggacgac agcgctcagc ccggctcctc tattccctga ctttgctggc
     1981 tcggcggata ttggtaaaac cttcgaacag gcatcacatg acatttcccc gccgccaatt
     2041 tctgtgcttg gctgccggcg ctgccgcgct cgcggccgtt ccgcgcatcg gtgcggcgca
     2101 aacctatccc gcgcgaccgg tgcgcgtgat cgttccattc gcgcccggcg gtccgaccga
     2161 tgtgttcgcg cgcctcatgg ctcaaaagct gtccgagcaa atgggcgcgc agttctacgt
     2221 cgagaacatc gccggtgcgg gcggcaatat cggagcgggc cgggcggcgc agtcggccgc
     2281 cgacggctac actctcctcg tcaacggcgc caaccacgtc gtcaatcccg cgctctatca
     2341 gcgagtgccc tacgatccca ccaaggattt cgacccggtc acgctggcgg taaccgcacc
     2401 tgcggtgctc actgtcaacc cgacgctgcc ggtgcacacg gtgcgcgacc tcgttgccct
     2461 catcaaagcc catcccggca aatacagcta ctcctcgccc ggggtaggca cgccgcccca
     2521 tctcgtgggc gagttgttcc gcctgtcgct cggcctcgat ctcgtacacg taccattcaa
     2581 cggcggcggc ccggcgatcg cctcggcggt cgcgggccac acgccgattt ccttcggctc
     2641 gacggctccg gccgtgccgc tggtcaaaga cgggaagctg cgggcgctcg cggtctcgac
     2701 caagacgcgg tcgccggcgc tgcccgacgt ccccaccatg acggaggccg gttatcctga
     2761 cgttgcgggc gaatcgtggt tcgccgtcct cgtgccggcc ggaacgccca aggagatcat
     2821 cgcgctgctg cagcggggga ttgcacaagc catcgcgggg cctgacatga aggaacggct
     2881 ggccaccctc ggctacgagc cggtcgccag cacgcccgcg gaatgcgccg cccggttccg
     2941 gacggaaata gcgaaatggg ggaaagtcat tggcgaggcg ggcatcaagg cgcaatagag
     3001 ccgcgccgat attcatccgc ttgcgcgagt ctgccccctt acttgcgcat tttgcagttg
     3061 ggcgcaggtc gccgatgcaa cgccaacggc cgatattgcg gattgcgtgt cactgacgaa
     3121 gcaaacgaaa aagttgcaga aacgaaatca tggcgcctgc cagcgcggcg aggatcgcaa
     3181 ccgtcttgat gagccaattt cgttcgagtt ccgcaatgcc ccgctgtgct cgctccctga
     3241 acgttgtcgt cccaggtagc gctcccttga acggccgatc gcctagaaac gacgtcactg
     3301 cgtcgaagaa aacccgcgta gcgggttcat ccgcgagaaa gatgtgattg cggctgtcca
     3361 gaggaacgaa tttggcgcgc ggaattccgg cggcgatttc ctggcccaac ccggccggcg
     3421 gcccatggca acgcgggcag tgtcctcgcc gctcattgcg ggatgacaaa ccccgaaata
     3481 gagccgccgc tcggccgctt cgacgcgaat ggcgtcgagc caaaaatcgg catgaactca
     3541 aggccttggg cgcattgggg ggacgacaaa cgaattctca attgatagat gtaatactaa
     3601 ttgtgcattg caatagctcg atgccgcagt gcactgaggg cgtgatcctc agccggtcgg
     3661 ttcaaccggg gtggcgctcg ggggagaaag ctatgaaaaa tcattgc
//
LOCUS       DATLJT010000367         5916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2088954,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000367 DATLJT010000000
VERSION     DATLJT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2088954"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VM910_08535"
     CDS             complement(<1..215)
                     /locus_tag="VM910_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVI62618.1"
                     /translation="MFDKILIANRGEIALRILRACKELGIATVAVHSTADDDAMHVRL
                     ADESVCIGPPPSKDSYLNIPALLAACE"
     gene            complement(239..733)
                     /gene="accB"
                     /locus_tag="VM910_08540"
     CDS             complement(239..733)
                     /gene="accB"
                     /locus_tag="VM910_08540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVI62619.1"
                     /translation="MARQPDDKTNAKFTSDDSALIRELALLLDETSLTEIEIERAGLR
                     VRVARNISIAASMQPGVAAIAAAAPATSATATPSAPVANFSNHPGVVPSPMVGTAYWA
                     PEPGAKPFIEVGTKVSAGQTLLIIEAMKTMNQIPSPRAGTVTQILVEDGQPVEFGEPL
                     VIIE"
     gene            complement(776..1255)
                     /gene="aroQ"
                     /locus_tag="VM910_08545"
     CDS             complement(776..1255)
                     /gene="aroQ"
                     /locus_tag="VM910_08545"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HVI62620.1"
                     /translation="MAKTPDKTAEQTIYVLNGPNLNLLGTREPQTYGHARLADVEKLC
                     METAAQFGLKADCRQSNREGELIDFIHEAHARQAAGIIINAGAYSHTSIALHDALKAV
                     KVPAVEVHISNIHARESFRHHSFTAIAAFAVLAGFGIDGYRLAINGLAAKIGARAKA"
     gene            complement(1518..2282)
                     /locus_tag="VM910_08550"
     CDS             complement(1518..2282)
                     /locus_tag="VM910_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HVI62621.1"
                     /translation="MTNRFLVSVAAIALIAGAGAANAQGGMNREGGGAAAQQSTPSGG
                     GSTGGATTQKSTEPSGMKSTQSEQKSPGAAKGQHAEDNAPGQKSKNMSTENETKGGAK
                     DMKAEGREGQDKNMKAEGRGDRDKNMKAEGRESRENNMKSEGRDTNTNAQTRTDERSH
                     TTTGQAGAGAKLSTEQRTRISTVIRNEHVSPVNNVDFAISVGTRVPRERISLRALPSE
                     VVTIYPEWRGYEFFLVRDEIVVVDPRSLEIVAVLPA"
     gene            complement(2470..3231)
                     /locus_tag="VM910_08555"
     CDS             complement(2470..3231)
                     /locus_tag="VM910_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI62622.1"
                     /translation="MPSLFVPALLGLALCGAPLSASAQSFSDPQRSEIEAIIKNYLIS
                     HPEVLEEATNELDKRKAAAEAKKHEETITENAEAIFNSPRGVVLGNKNGDVAFVEFFD
                     YNCGYCKRAMADMMDLLKNDPKLKVVLKEFPVLSEGSVEAAKVAVAVRMQDPTGKKYL
                     DFHQKLLGGRGLADKARAMAAAKEAGLDVARIEKDLASPEINDTIRENMKLAEDMGLN
                     GTPSYVIGKQIVVGAVGLDGLKEKIGIARCGKATC"
     gene            complement(3371..4783)
                     /locus_tag="VM910_08560"
     CDS             complement(3371..4783)
                     /locus_tag="VM910_08560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVI62623.1"
                     /translation="MLFDLTLRKRTSGLTAVTVALALALSPLASVRAEDHENRGPPVL
                     RDTETEQLLREYTRPILRVAGLEKQNIQMVIINDPSFNAFVADGRRIFVNYGAILKSE
                     TPNQVIGVLAHETGHLAGGHLAKLREQLAQAQTQMIIAMLLGAGAMVAGSRSSNPNNG
                     MANVGVAAIAGPQEMIRRTLLSYQRQQEENADRAGVKFLTATGQSAKGMYETFKRFTD
                     ESLFAAHGADPYLQSHPMPAERVAALEGLARASPYWDKKDDPSLQLRHDMVRAKISAF
                     MERQDTVYRRYPLSNTSLPARYARAITTYLHGDLNNALTQIDGLIQAQPNNAYLFELR
                     GQALLEGGRPKEAIAPLHKALQLSNSAPLIEMLLGQALVATENKAYTDEAITILRAAV
                     ARESEAPLGFSQLAMAYGRKGDYAEADLASAQAAYLRGDNKTARELASRAKTRFAVGT
                     PGWVKADDIVSAKPLPGQKN"
     gene            4939..>5916
                     /locus_tag="VM910_08565"
     CDS             4939..>5916
                     /locus_tag="VM910_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI62624.1"
                     /translation="MREATLRDRVAGLLGPSRRSNVPPFMVMDVMSAAERIEAAGGHV
                     IHMEVGQPAAPAPRTAIAAARAALDGGRIDYTSALGIPSLRERIARHYLDTYGCSVDS
                     GRIIVTTGSSGAFILAFLAMFEPGDRVAVTVPGYPPYRHILTALGCEPVLIETSGDTR
                     HALTGEALLAEHRKTPLKGVLVGSPANPTGTMMSREALTHLLEAAQSAGVRFVSDEIY
                     HGLDYAFPAVTAAELSDQALVINSFSKYFCMTGWRIGWMVVPEPLIRPIERLQQNLLI
                     SVPTLSQVAAEAAFDGRDEMEAIKRGYEVNRRILTEGLPAAGITAFLPAD"
BASE COUNT          995 a         1840 c         1844 g         1237 t
ORIGIN      
        1 atctcgcagg ccgcaagcag ggcggggatg ttgaggtagg agtccttgga cggtggcgga
       61 ccgatgcata cgctctcgtc ggcaagccgc acatgcatgg catcgtcatc cgcggtggaa
      121 tgcaccgcca cggttgcgat gccgagttcc ttgcaggcgc gcaggatgcg aagtgcgatc
      181 tcgccgcgat tggctatgag gatcttgtcg aacatgtatc cctattcgcc attcgctcct
      241 actcgataat cacgagcggt tcgccgaatt cgaccggctg gccgtcctcg acgagaatct
      301 gcgtcacggt gccggcgcgc ggcgagggaa tctgattcat ggtcttcatc gcctcgatga
      361 tcaacagcgt ctggcccgcg gacaccttcg tgccaacttc gatgaagggt ttggcgcctg
      421 gctccggtgc ccagtaggcg gtgccgacca tcggcgaggg caccacgccc ggatggttcg
      481 agaaattggc gacgggggcg ctcggcgtcg cggtagcgga ggttgcggga gccgcggcgg
      541 cgatggccgc cacgcccggc tgcatcgagg ccgcgatcga aatgttgcgc gcgacgcgca
      601 ctctgagacc ggcgcgttcg atctcgattt cagtcagact cgtttcgtcg agcagcagcg
      661 caagctcgcg gatgagcgcg ctgtcgtcgc tcgtgaattt tgcgttcgtt ttgtcgtctg
      721 gctggcgcgc catggtctct gatccaagct cttccaggtg tgggccgctg acgcgtcagg
      781 ccttcgccct ggcgccgatt ttggcggcaa ggccattgat cgcgagccga tagccgtcga
      841 tgccgaatcc cgcaagtacg gcaaacgcgg ctatggcggt gaaagaatgg tgacggaaac
      901 tctctctcgc atgaatattg ctgatatgca cctcgacggc agggaccttc acggccttca
      961 gcgcgtcatg gagcgcgatc gaggtgtgcg aataggcgcc ggcattgatg atgatgcctg
     1021 cggcttgcct cgcatgcgcc tcgtggatga aatcgatcaa ctcgccctca cggttggatt
     1081 ggcggcaatc ggccttgagg ccgaattgcg cggcggtctc catgcacagc ttctcgacat
     1141 ccgcgagccg ggcatgaccg taggtctgcg gctcccgtgt ccccagaaga ttgaggttcg
     1201 gtccgttgag gacatagatg gtttgctcgg cagtcttgtc tggggtcttg gccatcccac
     1261 tcccgataat gcgctcgcga acgtcaaatc cgctccactc gtgtggcgcg ttataggtaa
     1321 caaagcagca taggggaagc cttgagacct cgccggaggt ggtccaaatc cctcatcccg
     1381 cttgaaacaa ggcgcgtaaa acttgtggaa atcgccgatt aaccaacgat aaagcggcct
     1441 gaggcggggc tgggcccaga caaaaaggcg ggcccgtcgg ggcccgccaa aatgctctcg
     1501 ttgttgctcc tcgaccttta ggccggcagc acggccacga tctcaagcga ccgcgggtcg
     1561 accaccacga tctcgtcccg taccaggaag aactcatagc cgcgccattc cggatagatc
     1621 gtcacgactt ccgagggaag cgcccgcaag ctgatgcgct cgcgcggaac gcgcgtgccg
     1681 accgaaatcg cgaagtcaac attgttgacc ggcgagacgt gttcgttgcg gatcaccgtc
     1741 gaaatccggg tacgctgctc cgtcgacagc ttggcgcctg cgccggcctg acccgtcgtg
     1801 gtatgcgagc gctcgtcggt ccgggtctgc gcgttggtgt tggtgtcgcg gccctcggac
     1861 ttcatgttgt tctcacggct ctcacggccc tcggccttca tgttcttgtc gcggtcgccg
     1921 cgaccttcgg ccttcatgtt cttgtcctga ccctcgcggc cttccgcttt catgtccttc
     1981 gcaccgccct tggtctcgtt ttcggtgctc atgttcttcg acttctgacc cggcgcatta
     2041 tcctccgcat gctggccctt cgccgcgccc ggcgacttct gttcggactg ggtcgacttc
     2101 atgcccgaag gttccgtgct cttttgcgtc gtggcgccac ctgtgctgcc accgcccgac
     2161 ggggtgctct gctgcgccgc cgcgccgccg ccctcgcggt tcatgccgcc ttgggcgttc
     2221 gctgcgcctg cgcccgcaat cagcgcaatg gctgcaaccg aaaccagaaa gcgattcgtc
     2281 attcgagtat ctcctcacat gtctgcattg cccgcgccaa caacgatagg agatttgaga
     2341 tgttccactt ctctcgctca gttccgccgg gattgtttgt cgcggtggcc ggaacaaaag
     2401 cgcgcaaagc gaaagggccg gcattttcgg ccggcccttg cttgcaacgt ttcaaaaaga
     2461 aggatgcgct cagcaggtcg ccttgccgca acgcgcgatg ccgatcttct ccttcagccc
     2521 gtcgagtccg accgcgccga ccacgatctg cttgccgatc acgtagctcg gcgtgccgtt
     2581 caagcccatg tcttccgcaa gcttcatgtt ctccctgatc gtgtcgttga tctcgggact
     2641 tgcgagatcc ttctcgatcc tcgcgacgtc gaggccggcc tccttggcgg cggccatggc
     2701 gcgggccttg tccgcgagcc cgcggccacc caagagcttc tggtggaaat cgagatattt
     2761 tttcccggtg gggtcctgca tgcgcacggc caccgcgacc ttcgcagcct cgaccgaacc
     2821 ttcgctcagc accggaaatt ccttcagcac gactttcagt ttcggatcgt tcttcaggag
     2881 atccatcatg tcggccatcg cgcgcttgca gtagccgcag ttgtaatcga agaactcgac
     2941 gaaagcgacg tcgccgttct tgttgccgag cacgacgccg cgcggcgagt tgaaaatcgc
     3001 ttccgcgttc tcggtgatgg tttcctcatg cttcttcgct tccgccgcgg ccttgcgctt
     3061 gtcgagctcg ttcgtcgcct cctcgagcac ttcgggatgc gaaatcagat agttcttgat
     3121 gatggcctcg atttcgctgc gctgggggtc gctgaagctc tgcgccgaag cggagagcgg
     3181 cgcgccacac aaagcgagcc caagcagggc agggacgaag agactgggca ggcgcatggg
     3241 caatcctctg atcaaggccg gattcctgaa accgttatcg gccgtgtttc ggttaagctc
     3301 tatcggaatt tcggtgcaac gcaatttcgg ttgattcctg gctgcttttc ccaactcccg
     3361 gatgcatcga ctagttcttc tggcccggga gcggtttggc ggaaacgatg tcgtcggcct
     3421 tgacccatcc cggcgtgccg acggcgaagc gggttttcgc ccgtgatgcg agctcgcgcg
     3481 cggtcttgtt gtcgccgcga agataggctg cctgcgccga cgccagatcc gcctccgcat
     3541 aatctccttt tctgccgtag gccatcgcca actggctgaa accaagcggc gcctcacttt
     3601 cgcgcgccac cgcggcacgc aggatggtaa tcgcttcgtc ggtataggcc ttattctcgg
     3661 tggccaccaa ggcctgccca agtaacatct cgatgagggg tgcgctattg gagagctgta
     3721 gcgccttgtg cagcggtgcg atggcctcct tgggcctgcc gccttcgagc aatgcctggc
     3781 cgcgcagttc aaagagataa gcattgttcg gctgcgcctg aatgaggccg tcgatctggg
     3841 taagcgcgtt gttcagatcg ccatgcaggt aggtcgtgat cgcgcgcgca tagcgcgccg
     3901 gcaggctggt gttcgatagc gggtaacgcc ggtagacggt gtcctgccgc tccatgaacg
     3961 cggaaatctt cgcgcgcacc atgtcgtgcc gtagctgcag ggagggatcg tccttcttgt
     4021 cccaataggg gcttgcgcgt gcgaggccct cgagcgctgc gacgcgttcg gctggcatcg
     4081 ggtgggactg caaatagggg tcggcaccgt gtgcggcaaa caggctctca tcggtaaagc
     4141 gcttgaacgt ctcgtacatt ccttttgccg actgacccgt cgcggtcagg aacttcacgc
     4201 cggcacggtc ggcattttct tcctgctggc gctgatagga cagaagcgtg cggcggatca
     4261 tttcctgcgg acccgcgatc gcggcgacgc cgacattggc catcccgtta ttgggattgc
     4321 tgcttcgtga gcccgccacc atcgcgccgg caccgagcag catggcaatg atcatctggg
     4381 tctgcgcctg cgcgagctgt tcgcgaagtt tcgcaaggtg accgccggca aggtggccgg
     4441 tctcgtgcgc cagcacaccg atgacctggt tcggtgtctc cgacttcagg atcgcgccgt
     4501 agttcacgaa aatacggcga ccgtcggcaa cgaaggcgtt gaacgaggga tcgttgatga
     4561 tcaccatctg aatgttctgc ttctcgagtc ccgccacgcg cagaatcggg cgggtgtact
     4621 cgcgaagcag ttgctcggtc tcggtgtccc gcaggacggg agggcccctg ttctcgtggt
     4681 cctctgcgcg caccgaagca agcggcgaga gcgcaagggc gagggccacc gtgacggcgg
     4741 tcaatccgga tgttcttttg cggagcgtga ggtcaaaaag catggacggt cttggaagcc
     4801 cggtcttgta cttggcgatg gctacttggc gattggcggc aggccgaata cgggatatcc
     4861 cctaacaaac catggaatgc ggctagtctg gggcgcccct cccgaaggcc ggttcaaaag
     4921 ccgaaatagc agatatcgat gcgcgaagcg accttaagag accgtgtggc gggcctgctt
     4981 gggccgtccc gccgcagcaa tgttccgcca ttcatggtga tggacgtgat gagtgcggcc
     5041 gagcgaatcg aggcggcggg cggtcatgtc attcatatgg aagtgggcca gccggcagcg
     5101 ccggcaccgc gaacggcgat cgctgccgcc cgcgccgcgc tcgatggcgg gcggatcgac
     5161 tacacgtccg cgctcggtat cccttcgttg cgcgaacgta tcgcccggca ctaccttgat
     5221 acttacggct gctcggtgga ttcagggcgg attattgtca cgaccggctc ctcgggagcg
     5281 tttatcctcg cctttctggc catgttcgag cccggcgacc gcgtcgcagt cactgtgccc
     5341 ggttatccgc cttatcgtca cattctcacg gcactcggct gcgagcccgt gctgatcgag
     5401 acctcgggcg atacccgcca cgcgctcacc ggcgaagcgt tgctcgccga gcatcgaaaa
     5461 acaccgttga aaggtgtgct ggtcggcagt cccgccaatc cgactggaac catgatgtcg
     5521 cgggaagcgc tgacgcacct cctggaagcc gcacaaagcg cgggcgtccg gttcgtttcc
     5581 gacgaaatct atcatgggct cgactatgcc tttccggcgg tgaccgcggc cgagctttcg
     5641 gaccaggcgc tcgtgatcaa ttccttctcg aaatacttct gcatgaccgg ctggcgcatc
     5701 ggctggatgg tggtgccgga gccgctgatt cgcccgatcg agcggttgca gcaaaacctt
     5761 ctcatctcgg tgccgacgct gtcgcaggtc gccgccgagg cggcgttcga cggccgcgac
     5821 gagatggagg cgatcaaacg cggctacgag gtcaaccgcc gtatcctcac cgaaggcctg
     5881 ccggccgccg gcatcacggc gttcctgccg gccgac
//
LOCUS       DATLJT010000368         1788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_21155,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000368 DATLJT010000000
VERSION     DATLJT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21155"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..768
                     /locus_tag="VM910_08570"
     CDS             199..768
                     /locus_tag="VM910_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI62625.1"
                     /translation="MSRKLLLPYRQRVIGGAVGRVLEIGIGSGLNLSLYGDAVEEVIG
                     VDVSPELLTMAERVAQRRGLRVRLLEGSAEHLPSNIADGSIDTVVITWALCSIPDVAA
                     ALVEAHRVLRPNGSLRFVEHGRSPDTKVAWWQDRLTPLWRRCSGGCHLSRKTDDLLQS
                     AGFRLTHLATGYAPGPRPMAFMYEGQAEP"
     gene            939..1130
                     /locus_tag="VM910_08575"
     CDS             939..1130
                     /locus_tag="VM910_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62626.1"
                     /translation="MIQEIDIWRVAVLMLKRYADDAEGNADRRADELETEGDQAGAAI
                     WRRVTIAIEQLTDTSGPLH"
     gene            complement(1265..>1788)
                     /locus_tag="VM910_08580"
     CDS             complement(1265..>1788)
                     /locus_tag="VM910_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI62627.1"
                     /translation="IAGSALIATYPTARLATGLDPHVLRLAFALFLATIAATVAYHTW
                     RGGARVAAGPTRAWGWITVVGLIGGAFSGFFGVGGAFVAPPLLTTFFGLRQLEAQGLA
                     LALVSPSALVALLAYAGAGEVDWLVGLPLAFGGIAAISAGVKFASRLPERRMRLAFCG
                     LLLLTAVLLALRG"
BASE COUNT          373 a          553 c          550 g          312 t
ORIGIN      
        1 tacatggtgc gcgctcgatg accaagaccg actttgtggt cagccgtctg gaggcggagg
       61 acagcggttc cgccaggtag agacacgcgc gataatcggg catgatggac gatcgtcggc
      121 gcggaagctc aaaccggatc aatgtcgcga aagggccttt acgcacgata cgttcttcct
      181 cggctcaccg accttaccat gagccggaag ctgcttctgc cataccggca gcgtgtcatc
      241 ggcggtgccg ttggtcgggt gctggaaatc ggtatcggct cgggcctgaa tctgtcgctc
      301 tacggtgatg ccgtcgaaga ggtcatcggg gtagatgtct caccggaatt gctgactatg
      361 gcggaacgcg tagcccagcg ccgcggtctg cgagtccgat tgctggaagg ctcagcggag
      421 catctcccgt ccaacatcgc ggatgggagc atcgacacag tagtcatcac ctgggcactg
      481 tgcagcatcc ccgatgttgc agcggctctc gtcgaggcgc accgtgtgct ccgccctaat
      541 ggctcgcttc gctttgtcga acacggtcga tcgcccgaca ccaaggtggc ttggtggcag
      601 gaccgtctga ctccgctgtg gaggcgctgc tcgggcggct gtcatctgag tcggaagaca
      661 gatgatctcc tgcaatcggc ggggttcagg cttacccatc tggccaccgg ttatgcgccg
      721 gggccacggc ccatggcgtt catgtatgag ggacaggcgg aaccctaacg gctgaagcga
      781 acccggctcg acctcgaaat gtgatcggct ggctgatcgg accagggcac cccgccactg
      841 acacccccat caggctccga tgcggggcgt cctggttcaa cgaagccgcc ttttccccgt
      901 caagcagttc gaaacggagc caagctagac ttcccggcgt gatacaggag atcgacatct
      961 ggcgtgtcgc ggtgttgatg ctcaaacgct acgccgacga tgccgaaggg aatgccgacc
     1021 ggcgtgctga cgaacttgaa acagagggcg atcaagctgg tgcggcgatt tggcgtcggg
     1081 tcacaatagc gatcgagcag ctaacagaca catctggacc actccattaa tcatggttct
     1141 ggattagacc gagcgaattt atgcggcttc tgaatccttc cggccctcga ctacatcgcg
     1201 tcttgccagt gatcgtggcg gcaatgctct cctcgtcgaa acgcaatttg atactcggct
     1261 tcgatcaccc ccgcagcgcc agcagcaccg cggttagcag caagagtcca cagaatgcca
     1321 accgcatccg tctctcggga aggcggctgg cgaatttgac gccagcagaa atcgccgcga
     1381 tgccaccaaa cgcaagtggt aatccgacga gccaatcgac ttcaccggcc ccggcataag
     1441 ccagtaatgc gacaagcgca ctcggggaaa cgagcgcgag cgccagacct tgcgcctcca
     1501 actgccgcaa tccgaagaac gttgtcagta gcggcggcgc aacgaacgcc ccgccgactc
     1561 cgaaaaaccc cgagaacgcg ccaccgataa gcccgacgac cgtaatccag ccccaggctc
     1621 gcgtcggccc tgccgcaacc ctggcaccac cgcgccacgt gtggtaggcg acggtcgcgg
     1681 caatcgtggc gagaaagagt gcgaaggcga gccgcaggac atgcgggtcg aggccggtcg
     1741 ccaaacgggc tgtcggatag gtcgcaatca aagccgaccc ggcgatgg
//
LOCUS       DATLJT010000369         1689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1170391,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000369 DATLJT010000000
VERSION     DATLJT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170391"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VM910_08585"
     CDS             complement(<1..1197)
                     /locus_tag="VM910_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI62628.1"
                     /translation="MLQSAGYGAELAENPKRALELARRKDIQAAIVVQSRDLACLGQE
                     LRDKIPTTILLGHRTDDIVRPGHSLQETDAFPEDALDERKLLDLLSQAAALPGSKGDE
                     TVPAPLKIEDCKLDLAGHTFVDGSGREVRLTRAETALLTAFVDSPRRVLSRDQLRNAV
                     AGRGAEPYDRSVDMLVARLRRKIEPDPKAPRFILSVPGVGYKFDVRPQSAASGEASPA
                     LEPEPLNQSGGGEAIPAGTPSQGSAAPHSEPQRRQLTVLACELVDSTALAVKVDPEDF
                     GGTIRRFQEICTSVITHWGGAVINLVGNEILASFGYPKGYEDDAERAMHAGLDLVERI
                     GEVRSQSGDPLQVRVGIATGLVLVGENQPAIGDAVVMAARLRNITPPNSVTVTASTRK
                     LLGDMFV"
BASE COUNT          294 a          544 c          503 g          348 t
ORIGIN      
        1 aacgaacatg tcgcccagga gcttgcgtgt gctcgcggtc acagtgactg aatttggggg
       61 cgttatgttc cgcaatcggg cagccatgac gaccgcgtct ccgatggcag gctgattttc
      121 gccgacaagg actaagccgg ttgcaatgcc gacgcgcacc tgcaacgggt cgccggactg
      181 tgatcggacc tcgccgattc tttcaacaag atcgaggcct gcgtgcatgg cgcgctcggc
      241 gtcatcttcg tagcctttgg gatacccgaa cgacgcaaga atttcgttgc ccacaagatt
      301 gataacagct ccaccccaat gcgtgatgac tgaggtgcaa atttcttgga aacgtcggat
      361 ggtgccgccg aagtcctccg gatccacctt gactgcgagc gctgttgagt cgacgagctc
      421 gcacgccagc accgtcaact gccgcctttg tggctcggaa tgtggcgcgg cactgccctg
      481 gctcggcgtg cctgccggga tggcctctcc gcctccggac tggttgagcg gctccggctc
      541 aagggctggc gatgcctcgc cactcgcggc gctctgcggc cgtacgtcga atttatatcc
      601 gacgcctggc acggagagga tgaatcgtgg cgccttcggg tctggctcta tcttacgccg
      661 caggcgggcg acgagcatat cgacgctgcg gtcatagggt tccgcgccac gccccgcgac
      721 ggcgttacgg agctggtcgc gcgacagcac ccggcgagga ctgtccacga aggcggtcag
      781 cagcgcggtc tcggcgcggg tcagccgcac ttccctgccg ctgccatcga cgaaggtatg
      841 gccggccagg tcgagcttgc agtcctcgat ttttaacggc gccgggacgg tctcatcgcc
      901 cttgctccct ggcaacgccg ctgcttggct cagcagatcg agcagcttcc gctcatccag
      961 agcgtcttca gggaatgcat ccgtctcctg aagcgaatgg cccggccgga cgatgtcgtc
     1021 ggttcggtgc cccagcaaga tcgttgtcgg aatcttgtcg cgtaactcct gccccaggca
     1081 ggccaggtcg cgactctgca cgacgattgc cgcttggatg tctttacgcc tagcaagctc
     1141 gagcgcccgc ttcgggttct cggcgagttc ggcgccataa cccgcggatt gcagcacacg
     1201 agcaatccta gctcgaagct cgacctgttg cgctacaact aggacgttct tcctcactcc
     1261 gcagggtcct tctttcctag gcggccggag aagctgcaac gcccagcctc ccttggagtt
     1321 gatctaaatc aacgctgcga ccgtatatct gtctgggtcg gactcgttcc tgcatgtcgc
     1381 cagattaccc gaaagcggga cacggctggg cgatttatga gtacgcgctc gaggcattcg
     1441 ggcccaaatt tccacgctca ctcggcggcc aagtcaacgc gttaactttg tcttgcaacg
     1501 gctgcggccg ctgtgatgtc catcacaaaa tcgccgattg ggtggaaaaa ccgacctcgc
     1561 ggttggccga ctcccttgca attacgtagc ccggtaccac tcccgcaggt ggggttacgt
     1621 cgctgttgcc ccgctcgtgg gacaccaggc cggctgaacg cgcaacttcg cccactaatc
     1681 ccttcctct
//
LOCUS       DATLJT010000370         1745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1629457,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000370 DATLJT010000000
VERSION     DATLJT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629457"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..147)
                     /locus_tag="VM910_08590"
                     /pseudo
     CDS             complement(1..147)
                     /locus_tag="VM910_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            835..1275
                     /locus_tag="VM910_08595"
     CDS             835..1275
                     /locus_tag="VM910_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI62629.1"
                     /translation="MYIDHVGINTNDMEATLQFYCGILGMRLVRTTRTPDGRRHYNVE
                     IGGGNAFAVFDGAELPTAGERQYVNHLALPVDTSEEFDAAYQRLKDHGVAVTDIIERG
                     YGKTFYFHDPNGIRLQIELKTKQDANSLEGDPNPVPYVRLLQRA"
BASE COUNT          339 a          538 c          528 g          340 t
ORIGIN      
        1 gccggcatgc aatacggcga tgcgatccgc gagccccatc gcttccgtct ggtcgtgcgt
       61 cacatagatc gtggtcgtgc cggcttcgct cagcaccgac ttcaattcgg cccgcatctc
      121 gagccgcagc atcctcgcca aactcgaccg cttaccggtg ccgctccgtg gttcgctcct
      181 gactctgcgc tggagggcgg cggattcgaa ccatcggtcc cttacccgag tggcttgaaa
      241 aaggagccag agctttgtgc gcatggtcag ccgagatgcg tgcgcacccg tgaaatgcgc
      301 gtttatccaa gaccatgaca atcgggggcg cctatcgccc gtgcgatgca gccgtcgcct
      361 atgataatgg ccgaccattg tccgcttatg ccgtgcattg acggccggtt tggtccgatc
      421 tcgagaatcc gcccggctgt gattttgata cgtcccgccg gacccatcac ccgcgacggc
      481 gggctcctcg tcatcgatcc gccgacgttg ccgaagccaa cgcgcacgat tggatcgtgc
      541 ggaacaccgg cgctttggga tcgtcgcgcc ggcacggaat cgatcggggc tgcgggcggt
      601 cttgtccggt ggcgcatgaa ttgaatattg ctcgctgcaa ccgtgcgcgt cacgcgcgcc
      661 gggttgccga ccttcgcttg caaggccttc gcggcggccc tttggccgca atgatcgaga
      721 caaagcgtag catcgcacgc agcacgagct acaatgccag ccgaaacaag attcttcgcc
      781 tctagcacaa acgcgtgagg ctaactagac tagacctgcg aaggaggaac agcgatgtat
      841 atcgatcacg tcggcatcaa cacgaatgat atggaagcca cgctgcagtt ttactgcggt
      901 atactgggca tgcggttagt ccgcaccacg cgcacgccag atgggcgtcg gcattataac
      961 gtcgagatcg gtggcggcaa tgccttcgcg gtgttcgatg gtgccgagct cccgacggcg
     1021 ggcgagcgcc aatatgtgaa tcacctggca ctaccggtgg acacgtctga ggaatttgac
     1081 gccgcctacc aacggctcaa ggaccatggg gtcgccgtaa ccgacattat cgaacggggg
     1141 tatggcaaga ccttctactt ccacgatccg aacggcattc ggttgcaaat tgaattgaag
     1201 acgaaacagg atgcgaacag cttggaaggc gatcccaacc ctgtcccgta cgtgcgcttg
     1261 ctgcagcggg cttagtgctg cccaccgctc tgacatacgc cgcagatatc caacaagaac
     1321 tgaacgaatg ggaagccttg accgacgcgc cggcgaggtc gttgtcaaag gctgagtcgg
     1381 gtcgacacag accggtaccg tggggcgact cgtttgtccc atgcccgctc gtacaaagcc
     1441 ccgacagttc gcaggtgatg ctgttcgacg cgccggttac cttcacccct aggcgtgatc
     1501 ccggcaagct gctccaggac cgcgcatacc gcccggtgtc gtggccgccc cggccttgcg
     1561 caacacccgc gtggtaaaac tgtgttgcgg tggcgaagag cggcgtcggg caatgcaggt
     1621 cggcggcgaa tttgccgatg atgtcgaggt ccttcaaatg cgtcgtcatt gtcgccgttg
     1681 gctcgtcgta ttggcccctg accatcatcg gcccgcggat ctgaaacatg cgcgaggagc
     1741 ctgcg
//
LOCUS       DATLJT010000371         3784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3697470,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000371 DATLJT010000000
VERSION     DATLJT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3697470"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VM910_08600"
     CDS             <1..392
                     /locus_tag="VM910_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI62630.1"
                     /translation="IVAILLARLSFDVYAQIGLVVLIALAAKNGILIVAFAVEQRELG
                     KDTRAAAIEAAALRFRPVMMTSFAFILGLFPLVIAKGAGAITRQAVGTPVFGGMLAAS
                     LFGIFIIPLLYITAEGLRQRASRRKKK"
     gene            656..1627
                     /locus_tag="VM910_08605"
     CDS             656..1627
                     /locus_tag="VM910_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62631.1"
                     /translation="MPIIRASYAVAFAVALGLAAPAMAEDVINFGVQPSTQPILIAHG
                     AGYLKPIEEKYKIKINLRSFSYGAPENQALAAGELQIASAGMGPAILAAARLRATLVA
                     IDILDQTAILVPKDSKITTVAQLKGAKIAFPGEGSQQYPLLIKALADAKLDVKDVALF
                     KTDGSQVATLLANKSVDAGITWDPHVSRALADDVGRVLANSAQIMPIKNGHYVGDGTY
                     VRDDFMKARPDIVQDLITVQVKAIDLILSDPDKAIDIWTKENGFPRSVIEYAVKQKIS
                     VFDRNVIPDKSTVDAYTAFLKKAGLLKETDDPKFDTKFAQQALKDVK"
     gene            1644..2456
                     /locus_tag="VM910_08610"
     CDS             1644..2456
                     /locus_tag="VM910_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62632.1"
                     /translation="MTEAAAPAGAKPSGPAARLLRSTTRLLPYLYAAVGVVTVWWAVV
                     LIAKPHMSLLPSPLLTAQTFVQLVASGELLSHAAYSVCRVLAAWSLAAVVAVPLGFAM
                     GRWQRLERVLDPVVELFRPISPLAWIPLAILWFGIDEGGKIFVIFIGTFFPILLNTIA
                     GAKNIDPVLIRAGRILGCTDDASLFRKVVLPAAMPQVIVGLRISFGTGWAAIIAAELV
                     AARSGLGYLISNGMEVLRSDFVLVGMVTIGVLGVLFDAIFRWVDRCYNWMGE"
     gene            2537..3159
                     /locus_tag="VM910_08615"
                     /pseudo
     CDS             2537..3159
                     /locus_tag="VM910_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3521..>3784
                     /locus_tag="VM910_08620"
     CDS             3521..>3784
                     /locus_tag="VM910_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62633.1"
                     /translation="MPEPNLVGGSERDREQILQLHADYIDANARFDWGKLEPTWSAAP
                     EAVFFNLNGHTYKGREHWVRLWKFYGQNVQSSYWTPFDLGGVVS"
BASE COUNT          711 a         1153 c         1153 g          767 t
ORIGIN      
        1 cgatcgtcgc catcctgctg gcgcggctga gcttcgacgt ctacgcccag atcgggctcg
       61 tggtgctgat cgcgctcgcc gccaagaacg gcattctgat cgtcgcattc gcggtggaac
      121 agcgcgagct cggcaaggat actcgcgccg ccgcgatcga agccgccgcc ttaaggtttc
      181 gcccggtgat gatgaccagc ttcgcgttca tccttggtct ttttccgctg gtcatcgcca
      241 aaggcgccgg cgccatcacc cgccaagcgg tgggcacgcc ggtgttcggc ggcatgctcg
      301 ccgcatccct cttcgggatc ttcatcatcc cgctgctcta catcaccgcg gaaggtctgc
      361 gccagcgggc ctcgcggcgg aaaaagaagt gagggcggcg ctgtggcctc acactgagtc
      421 taggtcgtgt tgccgcctga cttggccacc gagccctcct ccgggcccag cctagctgtc
      481 gggtcggccg cgccgccgct gtaccttgac cgcagccggc cgcaccttca tctttctatg
      541 gtttcagttt tttgaccaaa cgggtatagc ctgtaaaaat cgatcggccc tagaaaccgg
      601 gcatgccggg ccgacgtagt atactatcgg agcagtctgt tcagaggggc aaagcatgcc
      661 gataatccgc gcttcgtatg ccgttgcgtt cgcagttgca ttggggctcg ctgctccagc
      721 gatggcggaa gacgtcatca atttcggagt ccagccttcc acgcagccga tcctgatcgc
      781 ccatggggcg ggatatctca agccgatcga agaaaaatac aaaatcaaga tcaatcttcg
      841 gtccttctct tacggcgcgc ccgaaaatca agcgctcgcc gccggtgaac tgcagattgc
      901 atccgcgggc atgggccctg ctattctcgc ggctgcgcgt ctgcgggcga ctctggtggc
      961 gatcgacatt ctggaccaga ccgcgatcct ggtgccgaaa gattccaaga tcacaaccgt
     1021 cgctcaactc aaaggcgcta aaatcgcctt cccgggcgag ggctcgcagc agtatccgct
     1081 gctgatcaag gcactcgccg acgccaagct cgacgtgaaa gacgtcgcat tgttcaagac
     1141 cgacggctcg caagtagcga cgctgctagc taataaaagc gtcgatgctg gcatcacctg
     1201 ggacccgcac gtttcgcgtg cgctcgccga cgacgttggc cgcgtgctgg caaactcggc
     1261 tcagatcatg ccgatcaaga atggtcacta tgttggcgac ggcacctatg tgcgcgacga
     1321 cttcatgaag gcgcgtcccg atatcgtgca ggacttgatc acggtgcagg tcaaggccat
     1381 cgatctgatc ctcagtgatc cggataaggc gatcgatatt tggaccaagg agaacggctt
     1441 tccgcgctca gtcattgaat acgcagtcaa gcagaagatt tcggtgttcg atcgcaacgt
     1501 cattccggac aagagcactg tcgatgccta caccgccttc ctcaagaagg ccgggctgct
     1561 gaaggaaacc gatgatccga agttcgacac caagttcgcg cagcaggcgt tgaaggacgt
     1621 aaaatgatgc tgccggcggc gcgatgactg aggccgccgc gcctgccggc gccaagccga
     1681 gcgggccggc ggcgcggctg ctgcgttcga ctacgcgatt attgccttat ctttatgccg
     1741 ccgtaggtgt tgtgacggtg tggtgggcgg tggtgctgat cgccaagccg cacatgtcgc
     1801 tgctgccgtc gccgctgctg acagcacaga ccttcgtcca gctggtggca agcggcgaat
     1861 tgctgagcca cgcggcttat agcgtctgtc gtgtgctcgc tgcttggtca ctcgcggccg
     1921 tggtggcggt gccgctcggc ttcgccatgg gccgctggca aaggctcgaa cgcgtgcttg
     1981 acccggtggt cgagctgttc cggccgatct caccactagc ctggattccg ctagccattc
     2041 tctggtttgg catcgacgaa ggcggcaaga ttttcgtcat cttcatcggc accttctttc
     2101 cgatcttgct caacaccatt gccggcgcaa aaaacatcga tccagtgcta atccgcgccg
     2161 gccgcattct cggatgcacg gacgatgcct cactgttccg caaggtcgtg ctgccggcgg
     2221 cgatgccgca agtgatcgtg ggattgcgca tctcctttgg taccggctgg gccgcgatca
     2281 tcgccgccga actggtcgcc gctcgctcgg ggctcggtta tctaatctcc aatggcatgg
     2341 aagtgctgcg gtcggatttc gttctggtcg gtatggtcac catcggtgtg ctcggtgtgt
     2401 tgttcgacgc gatcttccgc tgggtcgacc ggtgctacaa ctggatgggt gaatgatcgg
     2461 ccctaagccc aacctcgcta agccgttgtc actacgcggc ctcgccaagc gctacgcgac
     2521 tgccgacggc cgcacgatcg aggcgctctt gcctgttgac ctcgaaatca ctccgggcga
     2581 attcctggtg gtggttggtc cgtccggctg cggcaaatca accctgctca acattttagc
     2641 cggccttacc tcgccatcgg caggagaggc gtgggtgggt gacattcggg tggaagggcc
     2701 ggacatcgat cgcggcatgg tattccagga ctacgcgttg tttccatggc tggacgtgat
     2761 cggcaatgtg gaattcggcc tggagcgcaa gcgcatcccg cgccgcgagc ggcgcgggat
     2821 cgcaatgcgc tatctcgaga tggtggggct gaccgatttc gccgaaaagc gtccgcgtga
     2881 actatccggc ggcatgaagc agcgggtggc gatcgcgcga accttcgcga ccgagccttc
     2941 gattatcttc atggacgagc cattcggcgc gctcgatgca ctcacgcggc gccatctaca
     3001 gcatcaattg ctgcgcatct ggcagcagca ccggaagacc gtggtgttca ttactcattc
     3061 ggtggtggag gcgatctatc tcgctgaccg cgtcgtgctg atgaccgcgc ggccgggccg
     3121 tatcaagacg gtcctaagat cgaactgccg catccgcgcg atcccacctc ggacgaattc
     3181 cgctcaatcg agcgccgggt ctacgccgat ctggacgaag aactggtgaa aagcttccgc
     3241 atggagggca gcgataccgg tagcccgcca acagtgattt attgaagtga tctccgccac
     3301 acctgccgag gttgttttga ttacgaacgc ggactcaaac tgtgccagcc ccgacgcagg
     3361 cgatggtcat tttggccaaa cgaagccaaa cgcgcgagac gggcggcgag cattctggcc
     3421 aaacgaagcc aacgcatccc cggggacacg aggccgagac atcgtggtat gacggagctg
     3481 aactcccatc gggtgttgcg aactttgagt tggagagaaa atgcctgagc ccaatctcgt
     3541 cggcggctcc gagcgcgacc gcgagcaaat cttgcaactg cacgccgact acatcgacgc
     3601 caacgcccgc ttcgattggg gaaagctcga gccgacctgg agtgcggcgc ccgaggcggt
     3661 gttcttcaac ctcaatgggc acacgtacaa agggcgcgag cattgggtcc ggctgtggaa
     3721 attctacggg caaaacgtgc agtcgagcta ctggacgccg ttcgacctcg gcggcgtcgt
     3781 gtcc
//
LOCUS       DATLJT010000372         1965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2089227,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000372 DATLJT010000000
VERSION     DATLJT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089227"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VM910_08625"
     CDS             complement(<1..659)
                     /locus_tag="VM910_08625"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HVI62634.1"
                     /translation="MQETGVHNGAFGADKFGFRKLKALHWNFGAPQLYEHSLRAGEAT
                     LSADGALCADTGVFTGRSPKDKFTVRDALTDKSMWWAGNQSITSEQFDALYQDFLKHA
                     EGMTLFVQDLYGGADPALRIKTRVFTELAWHSLFIRTLLIRPEASELEGFVPELTVVD
                     LPSFRADPKRHGVRSENVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPEKGVMP
                     M"
     gene            complement(1371..>1965)
                     /locus_tag="VM910_08630"
     CDS             complement(1371..>1965)
                     /locus_tag="VM910_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HVI62635.1"
                     /translation="GSPILLISRLAVHTRNILADNRVSLMLDERAEGDPLEGARIMLL
                     GRAEEASEADLGHLRHRYLNAHPSAEAFVGFKDFSFFLVRLSGTHLVAGFGRIVDLKP
                     PQFLTEIGDATELLKAEQRAIAHMNQDHRDAMNLYVTRLLGAESADWRCTGCDPDGID
                     LQAGRTTLRLEFPERVTTSRELRTMLVRLAETARAKG"
BASE COUNT          386 a          616 c          601 g          362 t
ORIGIN      
        1 tgcatcggca tcacgccctt ctcgggcaga taatagttca gcgtggtgaa cacgctcttc
       61 ttcatctcgc ctgcataata agacccgccg atcaggacga tcttgcgggc gaaatcgatg
      121 gccacgacgt tctcggagcg cacgccgtga cgtttcggat cggcgcggaa gctcggcaaa
      181 tcgacgaccg tcaactccgg cacgaagcct tcaagctcgc tcgcttcggg acggatcaga
      241 agcgtgcgaa tgaacagcga atgccaggcg agttccgtga agacgcgcgt cttgatccgc
      301 aaagcaggat cggcgccgcc gtagagatcc tgcacgaaca gcgtcatgcc ttcggcatgt
      361 ttcaggaagt cctgatagag cgcatcgaac tgctctgacg tgatcgactg gttgcccgcc
      421 caccacatgg acttgtccgt gagcgcatcg cgaacggtga acttgtcctt cgggctgcgg
      481 ccggtgaaga cgccggtatc cgcgcacaat gcgccgtcag cggagagcgt ggcttcgccc
      541 gcgcgcaacg agtgctcgta gagctgaggt gcaccgaaat tccagtgcag ggctttgagt
      601 tttctgaaac cgaatttatc ggcaccgaag gcaccgttat gcacgcccgt ctcttgcacg
      661 aaaactcctc ctccaacccg cgaacgaaca aggcacgaat gccgccgagc gcgcctctcg
      721 cggtagcctc cattatagcc cgttcagggc taatactgat gaacgcccgt cttgccaagc
      781 cgatccagca tgatttggtt tattccgggg cagccctcaa aggcttgcgg cgtcgcaggc
      841 cgatatccgc ttcccgggac ctgtccaaag ccgtttttcg acgcgacaat cggccgcgag
      901 cagcagcaag tccgccccga cagccccttt tgcggtgcac aaaaggccgc ttttagcgcg
      961 cggcgcctcc cccgatcagg gcgaacgggc cccaggtgcc gcataggcat cctgggggac
     1021 gaggtgctgt tggggcaggc gagcatcgcc cgctgtaacg ctcccgcacc gcggaggccc
     1081 gggtaagtcg aagatcgagg tcacaagtcg gggcgcggcc taagagcaaa ccgaccaatg
     1141 cgttaccgac ccgcgcccgt gcgccggttc agaagaacga tcgtgggaga ccgcgtgagc
     1201 agcggctcac gtcggcatag cggctcctgt gttgatggag gtcggcgaga ttgtcgagcg
     1261 agcgtgcgac atcgcaatgg ttcacccgca cagcttctcg tgcatcgcgc gcgagcgctt
     1321 gatcaacggc tcgacgtcgc ttgtgcgcgc gttcgatggc gtcgaccggc tcaacccttt
     1381 gcgcgggccg tttccgccaa tctcaccagc atcgtgcgaa gttctctgga cgttgtgaca
     1441 cgctcgggaa attcgagccg cagcgtcgtc cggccggcct gcagatcgat gccatcggga
     1501 tcgcaaccgg tgcagcgcca gtctgcgctc tcggcaccaa gcaggcgcgt cacataaaga
     1561 ttcatggcgt cccgatgatc ttggttcatg tgcgcgatcg cgcgctgctc ggccttgagc
     1621 agttctgtgg cgtcgccaat ctcggtcagg aactgcgggg gcttgaggtc gacgatccgg
     1681 ccaaagcccg ccaccaggtg ggttcccgag agacgaacca ggaaaaaaga aaaatccttg
     1741 aaaccgacga aggcttcggc tgacggatgg gcattcaaat agcggtggcg aaggtgtccg
     1801 agatcagcct cgcttgcttc ctctgcccgg ccgagcagca tgattcgcgc gccctccagc
     1861 ggatcgccct cggcccgttc gtccagcatc agcgacaccc tgttatcggc gaggatgttc
     1921 ctcgtatgca ccgcaaggcg cgaaatcagc aggattggtg agccg
//
LOCUS       DATLJT010000373         1663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3008264,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000373 DATLJT010000000
VERSION     DATLJT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008264"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VM910_08635"
     CDS             <1..181
                     /locus_tag="VM910_08635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVI62636.1"
                     /translation="NSANSQFFIMFADGSFLNGKYTVVGEVVRGMDVVDKLKRGEPPA
                     NPDKIVHAQVAADMK"
     gene            complement(840..1292)
                     /locus_tag="VM910_08640"
     CDS             complement(840..1292)
                     /locus_tag="VM910_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVI62637.1"
                     /translation="MAARLDGGHEGAALLHRAEFAGAHCPSGRGRRERFAQFARPWIA
                     IGIGASHPDKDWSDATWSEFLRALRAPGTVFLVGGPANEARAEKFISVSNSGQIVNAC
                     GLRLIEAAALLRLADLFIGPSSGPLNLAAAGGTEAFGLFGSTPVLTYS"
BASE COUNT          326 a          514 c          538 g          285 t
ORIGIN      
        1 gaactcggcc aacagccagt tcttcatcat gttcgccgac ggctctttcc tcaacggcaa
       61 atacactgtg gtcggcgagg tcgttcgcgg catggacgtg gtcgacaagc tcaagcgcgg
      121 cgagccgccg gccaatccgg ataagatcgt ccatgcgcag gtcgctgccg atatgaaata
      181 gagcgtcaac cacgctccgc ctcggtgcag tgatgaaaca tcttctccgc tgcgccgctc
      241 tcctggtatt gacgctcttg gccggtttcg ctcacgcgac cgggcaggag cttcgcaagt
      301 gcgattttga cttaaagggc ggtcgtcgct ctggcgaggc acgcgtcacg cttgcggacg
      361 gcgcggtgat aagggtcgag gtggatgtaa tttggtgcgg ccggggcggg catcggaaaa
      421 gtctggacta tacatgtacc gtcaatacat cccgcggtga cggagagtcg acttggtcgg
      481 acgatgcggg cgcgacggtg atcaccgata gcactcccta tactgacccg cgtgaacccg
      541 accgcatcaa ggtgaccgtc ggacacgaag tgtcgatcca tctgaatgaa acgcaatcgg
      601 tcccaagatg cggcgtcgga gcggagctgc ccgaatccat cgtcattccg gcgcacaaat
      661 cggtttgtcg tgtgcggttc agagaacctt agcagctaag caccgcgctt cattactcgc
      721 tacgccggcg ctttgcggcg cgacagatga ggcgcgatcc gctctagcac ctgcgccggc
      781 gatatccgcg ccatgccgcc tggggaggga ccgcccttcg gtacgatggc ccggatgaat
      841 cacgaatagg tcagcaccgg ggtcgagccg aacaggccga acgcttcggt gcctccggcg
      901 gcggcgaggt tgagcggccc cgaactcggg ccgatgaaca gatcggccag ccgcaacagc
      961 gcggccgcct cgatcaatcg caaaccgcag gcattgacga tctgacctga attggaaacg
     1021 gagataaatt tttccgcccg cgcctcattg gctggtcctc cgaccagaaa gacggtgccg
     1081 ggcgcccgca gcgcgcgcag aaattcgctc caggtggcgt cggaccagtc cttgtctgga
     1141 tgcgaggcgc cgatcccgat tgcgatccag gggcgggcga attgcgcaaa cctctctctc
     1201 cgaccgcgcc cagaagggca atgggcacct gcaaattcgg ctcggtggag gagagcggca
     1261 ccttcatggc cgccatcaag gcgcgcagcc agtcgatcgg gtgatcgtga aaatgctcct
     1321 ggccaatgcc gtcattggtg atgaaggcgc tttgcgggcc gagtcccagc ccgatccgtt
     1381 ccggaatgcc ggcaagcgcc gctgccagcg ccggccggat ggtgcggtcg aggatccaga
     1441 gcgattgcag gtgatgccgg cgcaacagcg cgacgaggcg cacaagatta atgccgcgct
     1501 gccattccga tccggcatgc tgaaaataga ccgtggccgc gacgcgcggc tccgcagcga
     1561 gcaagtccct ggcggcgctc gtcggcggcg cgaagaacgt cacctgtccg tccggagcgg
     1621 ccgccgcgat ggcgcggacg aacggcaaat gccagatgac atc
//
LOCUS       DATLJT010000374         1965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2549352,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000374 DATLJT010000000
VERSION     DATLJT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549352"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1965)
                     /locus_tag="VM910_08645"
     CDS             complement(<1..>1965)
                     /locus_tag="VM910_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVI62638.1"
                     /translation="VVLAGLTPIAPTHEVVVTLLLANAVTVFLLVLVIAREVWGVVQA
                     RRRGRAAARLHVRIVALFSIVAAVPVIVVAVVASVTLHRGLDRLFSQQTQSLIENSLI
                     VADAYVREHVQFVRADSIAIAIELARAKPLFDQSREQFHQFLAVQASIRGLPAVMLLD
                     KDVNVVDQVDAQINEAFVKPSAEVLAAINDTEPQVAMFLDDNYVAAIIKLRGYNDLYL
                     YIARLLDPRVLAQLRATQASVAQYADLSARQIGIQIAFGLMYTVIALIVLLSAVWIGL
                     NFANYLVAPIRRLIGAAQVVSTGNLYVQVPTRRSEGDLAQLGETFNKMTQELRTQRDD
                     IVRARDLIDSRRRFTEAVLAGASAGVIGVDAESCISILNRSAERLIGRTEAEALGRPL
                     TEIVPELADMFAAARSGLQRLVQGQVTINRNGQERNLSVRVTSEQSGAAEGGYVVTLD
                     DITELVTAQRTSAWADVARRIAHEIKNPLTPIQLSAERLRRKYAGVIKEDGGIFQQCT
                     DTIVRQVDDIKRMVDEFSRFARMPKPVMTAEDVADTVRQVVFLQRVGNADIGIDLEIA
                     EDPMPARFDRRLISQALTNIIKNASEAIAAVPPQELGRGRIRVWAGREGNDIVIDVID
                     NGIGLPKENRSRLLEPYVTTRDKGTGLGLA"
BASE COUNT          343 a          617 c          657 g          348 t
ORIGIN      
        1 atggccagac cgagcccggt gcccttgtcg cgggtcgtga cataaggttc gagcagccgg
       61 ctacggttct ccttcggcaa tccgatcccg ttgtcgatca cgtcgatgac gatgtcgttg
      121 ccttctcgac cggcccagac gcggatacgc ccgcggccga gttcctgcgg cggcaccgcg
      181 gcgatggctt cgctggcgtt cttgatgatg ttggtgagcg cctgcgagat gagccggcgg
      241 tcgaaccgtg ccggcatggg gtcttccgcg atctcgaggt cgatgccgat gtcggcgttg
      301 ccgacgcgct ggaggaacac cacctggcgc accgtgtcgg ccacgtcctc ggccgtcatc
      361 accggcttcg gcatgcgggc aaagcgtgag aactcatcca ccatgcgttt gatgtcgtcg
      421 acttggcgca cgatggtgtc agtgcattgc tggaatatgc ctccgtcctc cttgatcacg
      481 ccggcatatt tccggcgcaa gcgctcggcg gaaagctgga tcggggtaag cggattcttg
      541 atctcgtgcg cgatgcggcg cgcgacgtcg gcccaggcgg aggtgcgttg cgcggttacg
      601 agctcggtga tgtcgtcgag cgtcactaca taaccgccct cggccgcgcc cgactgctcg
      661 ctcgtcactc gcactgacag gttgcgctcc tggccgttgc ggttgatcgt cacctgaccc
      721 tgaaccaggc gctgcagtcc ggagcgcgcg gcggcaaaca tgtcggcaag ctcagggacg
      781 atttcggtca gcggccgccc gagcgcctcg gcttcggtgc gtccgatcag tctctccgcg
      841 gaccgattca gaatgctaat gcagctctcg gcgtcaaccc cgatgacacc ggcgctcgct
      901 ccggccagca cggcttcggt gaaacggcgg cggctatcga tcaggtcgcg cgcgcgtacg
      961 atgtcgtcgc gctgggtccg cagctcctgc gtcatcttgt tgaacgtctc gcccagctgg
     1021 gcgaggtcgc cctcggagcg ccgggtcggc acttgaacgt agaggttgcc ggtcgagaca
     1081 acctgagccg cgccgatcag gcggcggatc ggcgccacca gatagttggc gaaattcagt
     1141 ccgatccaga ccgccgacag cagcacgatg agcgcgatca ccgtgtacat caatccaaat
     1201 gcgatctgaa tgccgatctg gcgcgccgag agatcggcgt actgagcgac gctggcctgc
     1261 gtcgcccgta gttgcgcgag cacgcgcgga tcgagcaggc gtgcaatgta cagataaagg
     1321 tcgttgtagc cgcgcagctt gatgatggcg gcgacgtaat tgtcatcgag gaacatcgcc
     1381 acttgcggct cggtgtcgtt gatggcggcc agcacctcgg ctgacggctt gacgaaggcc
     1441 tcgttgatct gcgcgtcgac ctgatcgaca acgttgacgt ccttgtcgag cagcatgacg
     1501 gccggcaatc cgcggatcga agcctgaacc gcgaggaact gatggaactg ctcgcgtgat
     1561 tggtcgaaca agggcttggc gcgcgcgagt tcgatggcga tggcgatgct gtcggcgcgc
     1621 acgaactgga cgtgttcgcg aacataggcg tcggccacga tcagcgaatt ctcgatgagc
     1681 gactgcgtct gctgcgaaaa caggcggtcg agcccgcgat ggagcgtgac gcttgcgacc
     1741 acggcaacca cgatcacagg gaccgcggcg acgatggaga acaaggcgac gatgcgcacg
     1801 tgcagtcgcg ccgcggcgcg cccccgacgc ctggcctgca cgacccccca cacctcgcgc
     1861 gcgatgacca gaaccagaag aaagaccgtg accgcgttgg caagcagcag cgtgaccacg
     1921 acctcgtggg tgggcgcgat cggcgtcagg ccggcgagca cgacg
//
LOCUS       DATLJT010000375         3846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_251428,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000375 DATLJT010000000
VERSION     DATLJT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251428"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VM910_08650"
     CDS             <1..700
                     /locus_tag="VM910_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HVI62639.1"
                     /translation="AAAKAVELIHQSKGELLMKGSLHTDELMREVTSSKTGLRTARRI
                     SHVFILDVPTYSETLFITDAAINIFPDLDAKRDIVQNAIDLFTQVGLGTPRVAILSAV
                     ETVTAKIPSTIDAAALCKMAERGQITGGILDGPLAFDNAIDPEAAQIKGIKSAVAGRA
                     QILVVPDLEAGNMLAKNLTFLAKADAAGIVLGTRVPIILTSRADSVRSRMASCAAAVL
                     YADARRRAAALPKA"
     gene            706..1950
                     /locus_tag="VM910_08655"
     CDS             706..1950
                     /locus_tag="VM910_08655"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVI62640.1"
                     /translation="MDAILVVNAGSSSVKFQIFDIEGGAPRRLRKGQIDGIGTRPRLT
                     AEANDKSSLIDRSYASDEIADVPAAIATVASWLRETQNFNLVAVGHRVVHGGPKYDRP
                     VVIDQAVMANLEQYVSLAPLHQPNNLAPIRTLMKSRPELLQVACFDTAFHHGHSAVAD
                     HFAIPEHFYAEGVRRYGFHGLSYEYVAQRLREIAPTVAAGRVIVAHLGSGASMCALAN
                     GQSVESTMGFTALDGIPMGTRPGQIDPGVLLYLLTEKGMEPTAVQDLLYRECGLKGLS
                     GISNDMRDLQSSSDPRATLAVDYFVYRVGLNAGMLAAALGGLDAFVFTAGIGENSPTI
                     RARIAQKIAWLGIVFDPAANAGRKSLISRPESRIVLLVVPTDEELMIAQHTLAFLPKH
                     TAKSGRAPWETYDKNTRQEEFP"
     gene            1947..3011
                     /locus_tag="VM910_08660"
     CDS             1947..3011
                     /locus_tag="VM910_08660"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HVI62641.1"
                     /translation="MTSDDLESVALTLVAKGKGILAADETVPTLTKRFNTLGIQSTEQ
                     SRRAYRELLFTSPGAAEFISGVIMYDETIRQKSSGGAPLAEALTVQGILPGIKVDTGA
                     KSLAGSPDETVTEGLDGLRDRLSEYRTMGAHFAKWRAVIHVTDALPSACVSANAHALA
                     RYASLCQEQKLVPIVEPEVLMEGAHTIERCEEVTGIVLHAVFNALFDQGVKLEGMLLK
                     PNMVVAGKACKRQPAVEEAATVTLRCLRRHVPAAVPGIVFLSGGQTARLATAHLNAIN
                     QLSGPKPWKISFSYGRALQDPALEAWHGRDENLAAGRSALYHRASCNGAASVGKYTDD
                     MELQSVSADDPPHRRGWRDD"
     gene            3135..>3846
                     /locus_tag="VM910_08665"
     CDS             3135..>3846
                     /locus_tag="VM910_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HVI62642.1"
                     /translation="MDAYWRAANYLSVGQIYLYDNPLLKQPLAKEHIKPRLLGHWGTT
                     PGLNFVYVHLNRVIKKHDLDMIYITGPGHGGPGLVANAYLEGTYSEVYPNISADEQGM
                     KRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYGAAFDNPDLIVACVVGDGE
                     AETGPLATSWHSNKFLDPLTDGVVLPILHLNGYKIANPCILARISHEELDQLFRGYGY
                     TPYFVEGDEPEKMHEAMAA"
BASE COUNT          772 a         1207 c         1150 g          717 t
ORIGIN      
        1 cgcggcggca aaggcggtcg agctgatcca ccaatccaaa ggcgagctgc tgatgaaggg
       61 cagcctgcac acggacgagc tcatgcgcga ggtgacctcg agcaagaccg gcctgcgcac
      121 ggcgcggcgc atcagccacg tgttcatcct ggatgtgccg acctattccg agacgctgtt
      181 catcaccgac gccgccatca acatcttccc cgatctcgat gccaaacgcg acatcgtgca
      241 aaacgcgatc gatctattca cccaggttgg cctcggcacg ccgcgggtcg cgattctctc
      301 cgccgtcgaa acggtgaccg ccaagatccc ttcgaccatc gacgcagccg cgctctgcaa
      361 gatggccgaa cgtggacaaa taacgggtgg cattctcgac gggccgcttg ctttcgacaa
      421 tgcgatcgat cccgaagcgg cgcagatcaa gggcattaaa tccgcggtcg caggtcgtgc
      481 gcagattctc gtcgtccccg acctcgaggc cggcaacatg ctggccaaga acctcacgtt
      541 cctcgccaag gcggacgcgg ccggaatcgt actcggcacg cgcgtgccga tcatcctgac
      601 gtcgcgcgcc gactccgtgc gctcgcgcat ggcctcctgc gcggccgcgg tcctctatgc
      661 tgacgcacgc cgacgtgctg cggcgttgcc gaaggcctga tcgctatgga tgcgatactc
      721 gtcgtcaatg cgggttcttc gagcgtcaaa ttccagatat tcgacattga gggaggcgca
      781 ccgcgccgcc tgagaaaggg ccagatcgac gggatcggca cgcggccgcg gttgacagcc
      841 gaggcgaacg ataaatcatc gctgatcgac agatcctatg cgtcggacga gatcgccgac
      901 gttcctgctg cgattgctac agtcgcttcc tggcttcgcg agacccagaa cttcaacctt
      961 gtcgcggttg gccaccgggt tgtccatggc gggccgaaat atgaccggcc tgtcgtcatc
     1021 gaccaagccg tgatggctaa tcttgagcaa tacgtttcgc tcgcaccgct gcatcagcca
     1081 aacaacctcg cgccgatccg cacactgatg aaaagtcggc ctgagttgct tcaggttgcc
     1141 tgcttcgaca ctgcttttca tcacggccac agcgccgtcg cggaccattt tgcgatcccg
     1201 gagcatttct acgctgaggg cgtcagacgc tacgggttcc acgggctctc ctacgaatac
     1261 gtcgcccagc gcttgcgtga gatcgccccg actgtggcag caggccgagt gatcgtcgcg
     1321 catctcggca gtggggcatc catgtgcgcc cttgcaaacg gtcaaagcgt cgaaagcacg
     1381 atgggtttca ccgcactcga tgggatcccc atgggcactc ggccgggcca aattgatcct
     1441 ggagtcctgc tttaccttct caccgaaaaa gggatggagc cgacggcggt ccaggacctc
     1501 ctctatcgag aatgcggtct gaaaggcctg tccggtatca gcaatgacat gcgagatctg
     1561 caatcgagtt ccgatcctcg cgcgacactc gccgtcgact atttcgtcta ccgggtcggg
     1621 ctgaatgccg ggatgctggc ggccgcgctg ggcgggctcg atgcattcgt gttcacggcc
     1681 ggcatcggcg agaactcgcc aacgatccgg gctcggatcg cgcagaagat tgcatggctg
     1741 ggcatcgttt ttgatccggc ggctaatgca ggtcgcaaat cgctcatttc ccggccggaa
     1801 agccggattg tgctcctcgt cgtaccgacc gacgaggagt tgatgatcgc gcagcatacg
     1861 cttgcgttcc tgccgaaaca caccgcaaaa tccgggcgtg ccccatggga gacctacgac
     1921 aagaacacaa gacaggagga attcccatga cgagcgacga tctggagagc gtcgcgctga
     1981 cgctggttgc gaagggaaaa gggatcctgg cggccgacga gaccgtcccc acgttaacga
     2041 agcgattcaa cacgctcggg atccagtcga ccgagcaaag ccggcgcgcc taccgggaac
     2101 tgctctttac gtcccctggc gccgccgagt tcatcagcgg tgtcatcatg tatgacgaaa
     2161 cgatccgtca gaagagttcc ggcggcgcgc cgctcgcgga ggcgctcacg gtccagggca
     2221 tccttccagg gatcaaggtc gacactggcg cgaagtccct tgccggctct cctgacgaga
     2281 ccgtcaccga gggacttgac ggcctacggg atcgcctctc ggagtaccgc accatgggtg
     2341 cgcatttcgc gaagtggcgg gctgtcattc atgtcacgga cgctctgccg tccgcctgcg
     2401 tgagcgcaaa cgcacatgcg ctggcgcgat acgcttccct ttgccaagaa cagaagctcg
     2461 tgccgatcgt cgagccggaa gtgctcatgg agggggcgca cacgatcgag cgctgcgagg
     2521 aggtcaccgg gatcgttctt cacgcggtgt tcaacgcgct tttcgaccag ggtgtgaagc
     2581 tcgaggggat gctgctcaag cccaacatgg tcgtcgcggg caaagcctgc aagcgtcagc
     2641 ctgctgtgga ggaggcggca acggtgacat tgcgctgcct gcgtcggcac gtgcctgccg
     2701 cggtgcctgg catcgtgttc ctgtcgggcg gccaaaccgc acgcctggcc actgcccatc
     2761 tgaacgcgat caaccagctg tctggcccga agccctggaa aatcagtttc tcttacggac
     2821 gtgcgcttca ggatccggct ctggaggcgt ggcatggccg ggacgagaat ctggcggctg
     2881 gccgaagtgc cctctaccac cgagccagtt gcaacggtgc agcgagcgtt gggaaatata
     2941 ccgatgacat ggagctgcag tcggtaagtg ctgatgatcc gccgcaccgt cgcggctggc
     3001 gcgatgacta atacccacac caccgataca cgcgaccatt ttccaccacg ttgaggcatt
     3061 cgatggcacg gaatcagaag acgaatcaga agacaaagac ggcaaatgct ctgtccaaca
     3121 aagagcgggc attgatggat gcgtactggc gagccgccaa ctacctgtcg gtgggtcaga
     3181 tctatctcta cgacaacccg ctgcttaagc agcccctcgc caaggaacac attaagccgc
     3241 ggctgctcgg gcactgggga accacgcccg gtttgaattt cgtctacgtt cacctcaatc
     3301 gggtgatcaa aaagcacgat ctcgacatga tctacatcac cggccccggg cacggcggtc
     3361 ccggtctggt cgcgaacgcc tacctcgagg ggacctacag cgaagtctac ccgaacattt
     3421 ctgccgacga acagggcatg aaacgcttgt ttacccagtt ctccttccct gggggtattc
     3481 cgagccatgt ggcgccggag acgcccggct cgatccacga aggcggcgaa cttggctacg
     3541 cactctcgca tgcctatgga gccgccttcg ataacccgga tcttatcgtt gcctgcgtcg
     3601 tcggcgacgg cgaagcggag accggaccgc tggcaacgag ctggcattcg aacaaatttc
     3661 tcgatcccct gaccgatggc gtagttctgc cgatcctcca tctcaacggt tacaagattg
     3721 ccaacccctg tattctcgcg cgcatcagcc atgaggagtt ggatcagctc ttccgcggct
     3781 atggctacac gccgtatttc gtcgagggag atgagccgga aaagatgcac gaggccatgg
     3841 ctgccg
//
LOCUS       DATLJT010000376         3607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1170688,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000376 DATLJT010000000
VERSION     DATLJT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170688"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1983
                     /locus_tag="VM910_08670"
     CDS             <1..1983
                     /locus_tag="VM910_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HVI62643.1"
                     /translation="RKEVEKWTPDEVERVTGLPEAQVKRVAEMFAKQRPSTLIWAMGQ
                     TQFSVGTANVRASCILLLATGNVGNFGTGANIFRGHCNVQGATDLGLDIVTLPLYYGL
                     VEGAWKHWCRVWEVDYDWMLSRFDSKQIMETSGIPSTRWFDATLLPKDQVAQKDNLKA
                     MLVFGHGGNTVTRMPQAAKGIEQLELLVVGDPHPTTWAVLGERKNDTYLLPICTQFET
                     SGSRTASNRSLQWGEQIVKPIFESKDDYQVMYLLAKRLGLADRMFKNIKVEGDRPVSE
                     DILREINRGGWSTGYCGQSPERLKLHMANQKDFDLVTLRAKDGPAKGDYYGLPWPCWG
                     TPELKHPGTHTLYNTNLHVMDGGSTFRARFGVEREVKLPDGTTRKDNLLAEGSYSLGS
                     AIQDGYPEFTYGVLKKLGWDQDLTPAEKAVIERIGGNNPDAVSWAIDLSGGIQRVAMA
                     HGCIHYGNGKARANAWNLPDPIPVHREPIYTPRADLVAKYPTRPDAKEFRVPNTGFTV
                     QKTAVEKGIAKQFPLILTSGRLVEYEGGGEETRSNKWLAELQQDMFIEINPADAAERG
                     IRDGAWVFVWGPEMESGKATRMKALVTERVGKGVTWCPFHFAGWFQTVDQRGKYPQGC
                     DPIVLGESVNTITTYGFDPVTGMQEPKATLCQVKAA"
     gene            1996..2592
                     /gene="fdh3B"
                     /locus_tag="VM910_08675"
     CDS             1996..2592
                     /gene="fdh3B"
                     /locus_tag="VM910_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HVI62644.1"
                     /translation="MARMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTINDG
                     KPGERSVTMACMHCTDAPCAAVCPVNCFYTTADAVVLHSKDLCIGCGYCFYACPFGAP
                     QYPKVGNFGSRGKMDKCTYCAGGPEPDGSLAEFEKYGTNRLAEGKLPLCAEMCSTKSL
                     LAGDGEIIAQIYKERVVKRGYGSGAWGWQTAYRETIAS"
     gene            2688..>3607
                     /locus_tag="VM910_08680"
     CDS             2688..>3607
                     /locus_tag="VM910_08680"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HVI62645.1"
                     /translation="MPKLSARLRFIVSAMALALIVAVAAPAAAQQRNPDSSVNPTASS
                     VNEQQLLSEFNRISGRCTLPDQRACTIQQPAGRDWREFHEVTLRWIGAIAILGILAIL
                     VAFYLTRGMVKLEAGRSGRVLVRFSTFERFVHWMTAACFILLALSGLNITFGRPLLLP
                     LIGPEAFTTWSQWAKYAHNYLSFPFTIGVFMIFLMWIGWNIPNRRDVEWLREGGGMVG
                     DKHPPADRFNAGQKMIYWIVVLGGTAVAVTGYILMFPFYGTNIANMQLAQIVHSVVAM
                     LFIAAMLGHIYIGTIGMEGAFEAMWDGTVDV"
BASE COUNT          683 a         1162 c         1146 g          616 t
ORIGIN      
        1 cgcaaggagg tcgagaagtg gacgcccgat gaagtggagc gcgtgaccgg tctgccggag
       61 gcgcaggtca agcgcgtcgc cgagatgttc gccaagcaga ggccttcgac cctgatctgg
      121 gcgatgggac agacacaatt ctcggtcggc accgccaacg ttcgcgcgag ctgcatcctg
      181 ctgctggcga ccggcaatgt cggcaacttc ggtaccggcg ccaacatctt ccgcggccat
      241 tgcaacgtgc agggtgcgac cgacctcggc ctcgatatcg tgacgctgcc gctttactac
      301 ggcctggtcg aaggcgcgtg gaagcactgg tgccgcgtct gggaagtcga ctacgactgg
      361 atgctatccc gcttcgactc caagcagatc atggagacat cgggcattcc atcgacccgt
      421 tggttcgacg cgaccctgct gccgaaagac caggtcgcgc agaaggacaa cctcaaggcg
      481 atgctggtgt tcgggcacgg cggcaacacc gtcacccgca tgccgcaagc ggccaagggc
      541 atcgagcagc tggagctgct cgtggtcggc gacccgcatc cgacgacctg ggctgtgctc
      601 ggcgagcgca agaacgacac ctacctcttg ccgatctgca cccagttcga aacgtcgggc
      661 tcgcgcaccg cgtcgaaccg ctcgctgcaa tggggcgagc agatcgtcaa gccgatcttc
      721 gagtccaagg acgactacca ggtcatgtat ctgctggcga agcggctggg cctcgctgac
      781 cgcatgttta agaacatcaa ggtcgaaggc gaccggccgg tctccgagga catcctgcgc
      841 gagatcaatc gcggcggctg gtcgaccggc tattgcggcc agtcgccgga gcgcctcaag
      901 ctccacatgg ccaaccagaa ggatttcgac ctcgtcaccc tgcgggccaa ggatggtccc
      961 gccaagggcg actattacgg cctgccgtgg ccgtgctggg gcacgcccga actcaagcat
     1021 cccggcaccc atacgctcta caacaccaat ctgcacgtga tggacggcgg cagcacgttc
     1081 cgcgcccgct tcggcgtgga gcgcgaggtc aagcttcccg acggcaccac gcgcaaggac
     1141 aacctattgg ccgagggctc ctactcgctc ggctcggcga tccaggacgg ttaccccgag
     1201 ttcacctacg gggtgctcaa gaagctcggc tgggatcagg acctcacccc cgccgagaag
     1261 gccgtgatcg agcgcatcgg cggcaacaat cccgatgcgg tctcatgggc gatcgacctc
     1321 tccggcggca tccagcgggt ggcgatggca cacggctgca tccactacgg caacggcaag
     1381 gcgcgcgcca atgcgtggaa cctgcccgat ccgattccgg tgcaccgcga accgatctac
     1441 acgccgcggg cggacctcgt cgccaaatat cccacgcggc cagacgccaa ggagttccgc
     1501 gtgccgaaca ccggcttcac cgtgcaaaag acggcggtgg agaagggcat cgccaagcaa
     1561 ttcccgctca tcctcacctc cggccggttg gtggaatacg agggcggcgg agaagagacg
     1621 cgttccaaca agtggctcgc cgagctccag caggacatgt tcatcgagat caatcctgcc
     1681 gacgccgccg agcgcgggat cagggacggc gcctgggtct tcgtctgggg gccggagatg
     1741 gagagcggca aggccacgcg catgaaggcg ctggtcaccg agcgcgtggg caagggcgtg
     1801 acctggtgcc cgttccactt cgccggctgg ttccagaccg tcgatcagcg cggcaaatat
     1861 ccgcaaggct gcgatccgat cgtgctcggc gagagcgtga atacgatcac gacctacggg
     1921 ttcgatcccg tcaccggcat gcaagaacca aaagccacgc tgtgccaggt caaggccgcg
     1981 taaggagact cgacgatggc tcggatgaaa tttctctgcg acgccgaccg ttgcatcgag
     2041 tgcaacgcct gcgtcactgc ctgcaagaac gagcatgaag tgccgtgggg catcaaccgc
     2101 cgccgcgtcg tcaccatcaa cgatggcaag ccgggcgagc gctcggtcac catggcgtgc
     2161 atgcactgca cggacgcgcc ctgcgcggcg gtctgtccgg tgaactgctt ctacaccacc
     2221 gccgacgccg tggtgctgca ctcgaaagac ctctgcatcg gctgcgggta ttgcttctac
     2281 gcctgcccgt tcggcgcgcc gcaatatccc aaggtcggca acttcggttc gcgcggcaag
     2341 atggacaaat gcacctattg cgcgggcggg ccggagccgg acggctccct ggccgagttc
     2401 gagaagtacg gcaccaatcg tttggctgaa ggcaaattgc cgctctgcgc cgagatgtgc
     2461 tcgaccaagt cgctgctcgc cggcgacggc gagatcatcg cgcagatcta caaggagcgc
     2521 gtggtcaagc gcggctacgg gtccggcgcc tggggctggc agacggccta tcgcgagacg
     2581 atcgcctcgt agaggagaac gagcgggctg cgtggccgcg tgaatgagcg ggatgaatcg
     2641 accacgcgtg tcgagtgatg gcacaatcga ttaagggaac tgcagagatg ccgaaactta
     2701 gtgctcgcct ccgtttcatc gtcagcgcga tggcgctcgc gctgatcgtc gccgttgcgg
     2761 ctcccgcggc cgcgcagcag cgcaatcccg acagctcggt caatcccacg gcgagctccg
     2821 tcaacgagca gcagcttctc agcgagttca accgcatctc cggccgctgc accctgcccg
     2881 accagagggc ttgcaccatc cagcaacccg ccggtcgcga ctggcgtgag ttccacgagg
     2941 tgacgctgcg ctggatcggc gccattgcga ttctcggcat actggcaatc ttggtggcgt
     3001 tctatttgac ccgcggcatg gtcaagctcg aagcaggacg ctccggacgc gtgctggtgc
     3061 gcttctccac cttcgagcgc ttcgtgcact ggatgaccgc ggcctgtttc attcttctcg
     3121 ccctgtccgg actcaacatt accttcggca ggccgctgct cttgcccctg atcgggcctg
     3181 aggcgttcac aacctggtcg caatgggcga aatacgcgca caactatctg agcttcccgt
     3241 tcaccatcgg cgtgttcatg atcttcctga tgtggatcgg ctggaacatc cccaaccggc
     3301 gcgacgtcga atggctcagg gaaggcggcg ggatggtcgg cgacaagcat ccgccggccg
     3361 atcgcttcaa tgccggccag aagatgatct attggatcgt tgtgctcggc ggcacggcgg
     3421 tggcggtgac cggctatatc ctgatgttcc cgttctacgg gaccaacatc gccaacatgc
     3481 agctggcgca gatcgtccat agcgtggtcg cgatgctgtt catcgccgcc atgcttggcc
     3541 acatttatat cggcaccatc ggcatggaag gcgcgttcga ggcgatgtgg gatggcacgg
     3601 tggacgt
//
LOCUS       DATLJT010000377         3009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3008453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000377 DATLJT010000000
VERSION     DATLJT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3008453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..868)
                     /locus_tag="VM910_08685"
     CDS             complement(137..868)
                     /locus_tag="VM910_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62646.1"
                     /translation="MSALLRVSDVHISYGKVEAVRGASLEVGDNEIVTIIGANGAGKT
                     TLLNSIMGILPLRGRVDFAGQDLGRREIEDRVALGVSLVPEHRELFATMNVEDNLLLG
                     AFRAERAVVATSFERVYALFPRLKERRRQLAGTLSGGEQQMLAMGRALMGAPKLLMLD
                     EPSLGLAPIIVADIFRTIGELRSSGVSVLLVEQNAQAALAVADRAYVMELGELVLEGK
                     ASEMATNERVAASYLGFQHEGQSAV"
     gene            complement(865..2634)
                     /locus_tag="VM910_08690"
     CDS             complement(865..2634)
                     /locus_tag="VM910_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI62647.1"
                     /translation="MRERLPVLFFAVVMAAIPFVPGIPPFWIVLLDNIGLAALVAMGL
                     VLLTGVGGLTSFGQAAFCGFGAYTTAVLTTAFGLSPWLALPASLLVSGIAAVLLGLVT
                     VRLSGHYLPLGTIAWGLGLFYLFSKLEFLGRNDGISGIPPLSIGSFKMIDPGTIYYAI
                     WTAVLISALLTMNLLDSRTGRAIRALRRGHIAAEAFGVRTPRTKLLVFIYAAVLAGLS
                     GWLYAHFQRATNPTPFGAQAGIEYLFIAVVGGAGYVWGGVLGAAIVVILKEVLQSYLP
                     MLLHGEGQLETIVFGILLVVLLQLAPSGVWPWLMSLVPLKPRRTSTDTTLALPARLPA
                     AGAQGALLEVKNARKEFGGVIAVNDVSFDVRPHEIVALIGPNGAGKSTTFNLITGVLR
                     ATAGSISVLGRKTFNAPPQDIVRLGISRTFQHVKLVPDMSVLENVAIGAHLRGHAGAL
                     KSMFRLDRTDEAKLLAEAARQIERVGLSSQIDQPAGSLSLGQQRIVEIARALCVDPML
                     LLLDEPAAGLRHMEKQRLAALLRELRDGGMSVLLVEHDMGFVMNLADRVVVLDFGTKI
                     AEGTPQAIKTNPEVIKAYLGVTA"
     gene            complement(2641..>3009)
                     /locus_tag="VM910_08695"
     CDS             complement(2641..>3009)
                     /locus_tag="VM910_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62648.1"
                     /translation="LRATAVNRLGARLAGIRTTLSGQIAFLLAALIGALSGILIVPIT
                     TLYYDTGFLIGLKGFIAAIIGGLVSYPLTAIAALVVGTVEAFSSFYASNFKEVIVFTL
                     LVPVLLLRSLAAPAVEEEKD"
BASE COUNT          589 a          946 c          996 g          478 t
ORIGIN      
        1 agattgcgag ctttatcgca gtcctgttta tcacgcgaca atccctgctt ttccgtcagc
       61 acagagtgcg tgcggttgcg cgaaaagtaa agcggtcgcg cgcttcacac ttgcgggagt
      121 gttgcgtaca tttcgttcag accgcgcttt gtccctcatg ctggaagccg agatagcttg
      181 cggcgacgcg ctcattcgtc gccatctccg aagccttgcc ctcgagcacg agttcgccga
      241 gctccatcac ataggcgcgg tcggcgacgg caagcgcggc ttgtgcattc tgctcgacca
      301 ggagcaccga aacgcccgaa gaacgcaatt cgccgatggt tctgaaaata tcggcgacga
      361 tgatcggcgc aagcccgagg cttggctcgt ccagcatcag gagtttcggg gcgcccatca
      421 gcgcgcggcc catcgcaagc atctgctgct cgccgccgga aagcgtgccg gcaagctgcc
      481 ggcgtcgttc cttcaaccgc gggaacagcg catagacgcg ctcgaacgag gttgcgacga
      541 cagctctttc cgcgcgaaag gccccgagca gaagattgtc ctcgacattc atggtggcga
      601 acagctcgcg atgctccggc acgaggctca ccccgagagc gacccggtcc tcgatctcgc
      661 gccggccgag gtcttgcccg gcaaaatcga cgcggccccg gagcggcagg attcccatga
      721 tgctgttgag cagcgtggtc ttgccggcgc cattggcgcc gatgatggtg acgatctcgt
      781 tgtcgccgac ctcgagcgat gcgccgcgca cggcttcgac cttgccgtag gagatgtgca
      841 cgtcggagac ccgcagcagc gcgctcatgc cgtcaccccg agataggcct tgatcacttc
      901 ggggttggtc ttgatcgcct gcggcgtccc ttcggcgatc ttggtgccga aatcgagcac
      961 gacgacgcga tcggcgagat tcatgacgaa ccccatgtcg tgctcgacca gcagcaccga
     1021 catgccgccg tcgcgcagtt cgcgcagcag ggcggccagc cgctgcttct ccatgtgccg
     1081 caagccggcc gccggctcgt cgagcagaag cagcatgggg tcgacgcaga gggcgcgcgc
     1141 gatctcgacg atgcgctgct ggccgagcga gagcgagccc gcaggctggt cgatctggct
     1201 ggacaggccg acgcgctcga tctgacgcgc ggcttccgcc agcaatttcg cttcatcggt
     1261 gcggtcgagg cgaaacatgc tcttgagcgc gccagcgtgc ccgcgcagat gcgcgccgat
     1321 cgcgacattc tccagcacgc tcatgtcggg tacaagcttc acatgctgga aggttcggga
     1381 aatacccagc ctgacgatgt cctgcggcgg cgcgttgaac gtcttcctgc cgagcacgga
     1441 gatggagccg gccgttgcgc gcagtacgcc ggtgatgagg ttgaaggtcg tgctcttgcc
     1501 ggcgccgttg gggccgatga gcgccacgat ctcgtgaggc cgtacgtcga acgacacgtc
     1561 gttgaccgcg atcacgccgc caaattcctt gcgcgcgttt ttgacctcga ggagtgcgcc
     1621 ttgtgcgccc gccgccggca gtcgggcagg gagcgcaagc gtggtatcgg tggacgtgcg
     1681 gcgcggcttg agcggcacca gcgacatcag ccatggccag acgccgctcg gcgcgagctg
     1741 cagcagcacg acaagcagga tgccgaacac gatggtttcg agctggcctt cgccgtgcag
     1801 cagcatcggc aaatagctct gcagcacttc cttgaggatg acgacgatgg cggcgcccag
     1861 cacgccgccc cagacatagc cggccccgcc gaccactgcg atgaagagat attcgatgcc
     1921 ggcctgcgcg ccgaacggcg tcggattggt ggcgcgctgg aaatgcgcat agagccagcc
     1981 ggagagcccg gccaggaccg ccgcataaat gaagacgaga agtttggtgc gcggcgtccg
     2041 cacgccgaag gcttcggccg cgatatgccc gcgccggagc gcccggatgg cgcggccggt
     2101 gcgggaatcc agcaggttca tcgtgagaag tgccgagatc agcacggcgg tccagatcgc
     2161 ataatagatc gtgccgggat cgatcatctt gaacgagccg attgagagcg gcgggatgcc
     2221 ggagatgccg tcgttgcggc ccaaaaattc gagcttgctg aagaggtaga acaggccaag
     2281 gccccaggcg atggtgccaa gcggcagata atgaccggag aggcgtacgg tgacgaggcc
     2341 gagcaggacc gcagcaattc cgctgaccaa aagcgaggcc ggcagcgcca gccatggcga
     2401 gaggccaaaa gcggtggtca gtactgccgt ggtgtaggcg ccaaaaccgc agaatgccgc
     2461 ctgtccgaac gaggtcagcc cgccgacgcc ggtcagcagc acgagcccca tcgcaaccag
     2521 cgcggcaagg ccgatattgt cgagcagtac gatccagaat ggcggtatcc cgggcacaaa
     2581 gggaatcgcg gccatcacca cagcgaagaa gaggaccgga agacgctccc gcattgcgcg
     2641 tcagtccttc tcttcctcga ccgctggagc ggccagcgat cgcaacagca gcaccggcac
     2701 cagaagcgta aagacgatga cctccttgaa attggaggca tagaacgagg agaaggcttc
     2761 caccgtgccg acgacgagcg ccgcgatggc ggtcaagggg tagctgacga ggccgccgat
     2821 gatcgcggcg atgaagccct tcaacccgat caggaatccg gtgtcgtaat agagggtcgt
     2881 gatcggcacg atcaggatgc ctgacagcgc cccgatcagc gcggccagca ggaaggcgat
     2941 ctggcccgac aacgtggtcc tgatgccggc gagccgcgcc ccgagccggt tgacggcggt
     3001 cgcgcgcaa
//
LOCUS       DATLJT010000378         6155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1629864,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000378 DATLJT010000000
VERSION     DATLJT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629864"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM910_08700"
     CDS             <1..402
                     /locus_tag="VM910_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HVI62649.1"
                     /translation="LSATMTTTYGFGFDDLARPRSRGARIARIDALATLLDTALVIPG
                     TSVRFGLDALIGLFPVVGDIITTALSLFIVHEAYQLGAPGHVVARMLGNVALDGVFGA
                     VPLVGDAFDVLWRANRRNVLLLREWLDHERR"
     gene            422..844
                     /locus_tag="VM910_08705"
     CDS             422..844
                     /locus_tag="VM910_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HVI62650.1"
                     /translation="MRPMSLLLNVLWIVFGGLWMALGWVIAAVVMAITIVGLPWARAA
                     FTIAAYTLLPFGYKAVPRDEYFGREDVGTGPLGVIGNIVWLLLAGWWLALGHLVTAIL
                     LAVTIVGIPFAWAHLKLAGLALWPIGKAIVPVQNAPLG"
     gene            complement(1176..1412)
                     /locus_tag="VM910_08710"
     CDS             complement(1176..1412)
                     /locus_tag="VM910_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62651.1"
                     /translation="MQGIEKDDAEMSFRRGYQQGVIEVFYALERSLDPATRDVLQAWI
                     EKDIYVWRIKAMLGYPPVWRLKMLAGPRSAGAAL"
     gene            complement(1466..1669)
                     /locus_tag="VM910_08715"
     CDS             complement(1466..1669)
                     /locus_tag="VM910_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62652.1"
                     /translation="MPTTILICGSPADGFQVVGLFADSGDAESYAEDHSLRDWWVAEL
                     TSPEAFAESQRRQLQAPRGASWS"
     gene            complement(1873..2064)
                     /locus_tag="VM910_08720"
     CDS             complement(1873..2064)
                     /locus_tag="VM910_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62653.1"
                     /translation="MRNASLFAFTPALIAIGFGVWAASPANAPVSPSISQGIEPFKLM
                     VNAKELPTVEFADYTFVFH"
     gene            2934..3161
                     /locus_tag="VM910_08725"
     CDS             2934..3161
                     /locus_tag="VM910_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62654.1"
                     /translation="MSITPYLGDFDANPETKRVLGVALEMTRVSLGLADDFADGIIAK
                     RIVDRAKAGERNPDLLCQGALDELRGHLYGD"
     gene            complement(4029..4655)
                     /locus_tag="VM910_08730"
     CDS             complement(4029..4655)
                     /locus_tag="VM910_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62655.1"
                     /translation="MFGMLDFRAHKLYWLLTFPFRLTNWVILFAAIPVGIVVARSTAY
                     AWWAKIMVAYLVFGLALIIGGLLLGGSLWLFSRTFFWIVDIVPSKGSDDDEARAVVLY
                     GEHPLLLKKLDSKIEDWTEKDTEKLASYTLWRCRPFFRNNSKERFRARVRFLQQYYEE
                     TGARIGELAPSKRDELLKPYKDPWYEHSLQDPIIVNIFVASVFILTNV"
     gene            5353..>6155
                     /locus_tag="VM910_08735"
     CDS             5353..>6155
                     /locus_tag="VM910_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62656.1"
                     /translation="MTVTIGRRELLAALGGAAAAWPLAARAQQTHLITSPRVAFLGAE
                     SGSTNQHFFDAFRQGMHEHGYVDGQNITFVERWAEGRSERFPEIIEELISLKADVILA
                     LSLPAAQAAKSATTTVPIVFIASDPLGSGLVSSLARPAGNLTGFSLALGDEFSSKWLE
                     LLKEAVPSISRVAVLWNPVNPASSHYVTVLRDAAEKLGVRVQPQAVSDPDQFNGAFAT
                     IVAERAQALIVVVDPVTARYRERVVELAMKSRLPAMYGFREFVDAGGLIA"
BASE COUNT         1233 a         1860 c         1826 g         1236 t
ORIGIN      
        1 ctctcagcca ccatgaccac cacctatggc ttcggcttcg acgacttggc gcgcccccgc
       61 tcccgtggcg ctcgcattgc ccggatcgac gcgcttgcga ctctgctcga caccgcgctc
      121 gtcataccgg gcaccagcgt gcgcttcggc ctggatgcgc tgatcgggct ctttcccgtc
      181 gtcggcgaca tcatcaccac ggcgctgtcg ctgttcatcg tgcacgaggc ctaccagctt
      241 ggcgcgccgg gccacgtcgt cgcgcgcatg ctcggcaacg tcgcgctcga cggtgtgttc
      301 ggcgcggtgc cactggtcgg cgacgccttc gatgtgttgt ggcgcgcgaa ccggcgaaac
      361 gtgctgctgt tacgggagtg gctggaccac gagcgccggt gagcgtgtcg ttcgtcggtc
      421 gctgcgtccg atgtctctcc ttctcaacgt gctctggatt gtcttcggcg gcttgtggat
      481 ggcgctcggc tgggtaatcg ccgccgtcgt catggcgatc acgattgtcg gcttgccctg
      541 ggcaagagcg gcgttcacca tcgccgccta cacgctgctg ccattcggct acaaggccgt
      601 accgcgggac gaatatttcg gccgcgaaga tgtcggcacc ggcccgctgg gggtgatcgg
      661 caacatcgta tggctgcttc tcgccggatg gtggttggcg ctcggccacc tggtcaccgc
      721 gatcctgctc gccgtgacca tcgtgggaat cccgttcgcc tgggcacacc tgaaactcgc
      781 cgggctggcg ctgtggccga tcgggaaggc gatcgtcccg gtgcagaacg cgccgctcgg
      841 gtagcagcta ggctggcgct gcgacgtttc atgggggctc ctagtcgggc tcggtatact
      901 tggcacgacc gataagattt ccgcgaaggg agcggggtcg ggtgcttggg agtgcagatg
      961 tcgataggga cacagagtgc gatgaagcag tcgtggttac gaagtcgaag aagacaccga
     1021 cggctccaac gcacaaataa acgtgcgcgc tacgcgggac ttaaaccggg aatgggttgc
     1081 cccatgctgc cgccaaggcc gccgcctccg aggccaccga agccgccacc gccgcccccc
     1141 ccgcgccgca cagctgcttg tttctgagta gccaactaca gagcggcgcc cgctgacctt
     1201 ggaccagcta acatttttaa tcgccaaacc ggcggataac cgagcatcgc cttgatccgc
     1261 catacgtaaa tgtccttctc aatccaagcc tgcaaaacgt cccgcgtagc aggatcaaga
     1321 gaccgctcca gggcatagaa cacctcgatt accccctgct gatagccgcg ccgaaatgac
     1381 atttccgcgt catccttctc aataccctgc atatccgctt tcctctcttc tgatttccgc
     1441 gataaggggc tcgccctctg gctcatcacg accagctcgc accgcgcggt gcttgaagct
     1501 gtcgccgctg gctttcggcg aacgcttccg gtgatgttag ctcggcaacc caccaatctc
     1561 tgaggctatg gtcttcggca taggattcag catcgccgct atcggcaaac agcccgacaa
     1621 cttggaagcc atccgcaggg ctgccgcaga tcaagattgt cgtgggcatc tagctgctca
     1681 gatcggagtg gagattggcg gctgccgacg ctaacgagaa ctactgcttt gaatgaggat
     1741 tcgacttggg aaacaccatg gacacctacg tatgtccggc gaaaagatcc ggcgtcattt
     1801 gcgacgccgg gcggaaacca tcaaggggtt ggccaacgcc tcgtggcgca agagactccg
     1861 tttgttggcc gttcaatgga acacgaaggt gtagtcggca aactccaccg taggcagttc
     1921 cttcgcgttc accattagtt tgaacggttc aatcccctga cttatcgagg gggaaacagg
     1981 cgcattggcg ggcgaggccg cccatactcc gaatccaatc gcgatcaagg cgggggtaaa
     2041 ggcaaacagg ctggcattgc gcatctgagt tctcccgttc aatcagcagc ccgcaaagcg
     2101 gtagctcagc cgtctgatga cgagtgatat gccggaccct ggtttcgaat gatgtgatgg
     2161 ccatcacgaa atggtcgatt tcaggctcag aaaaatcggt gcccgccaac acagagagac
     2221 aaagaaagcc ccggtccgcc cggggcttct agtcgtccct aacaaaggag gaaccctaga
     2281 gcgacctgag tctctctaga cgccccgata ctatcccaaa gcacaaatca ggcaaggcgc
     2341 ggagcgcgta gacgcgcggc ggctatagtt tttagctatg accacggaca catgtttctg
     2401 cgcgctcgac gatgtattcg ccaaattcgt gacggccatc acaggcagcg gctgcatgaa
     2461 gctgctatgg cagcaaagtg acctcgaagc ctagggggct tcaatgatga ccaagggcat
     2521 aatcgctgtc gccgccgcgt tcttgttcgc tttgacggtt ggcattctga ccgcacagag
     2581 cggcaatgac agggctagca atcccacaat ccgcacgctt gcgttgcaat actaggaggc
     2641 agggctaact gcaaccaatc cagcttgctc agcgcgatga agagggaagc ggaggaggat
     2701 tgggggcgct gactagtagc tagctggcgg ctagcacctg caagcgggct ggcgaagcga
     2761 tcatcgcggc gcaagagcga gcgattgact gcagcgcact ttgagaaccg ctgcttggac
     2821 gcctcagaga aacgagaccg tgcataaacc tcatttattg tttcgataaa actaatgatg
     2881 ttgcagcgca aattgttttt tgccagatat ctgctgctac gtcggaggcc agcatgtcca
     2941 tcacgcctta tctaggcgac ttcgatgcca atcccgaaac taagcgggtt ctgggcgtcg
     3001 cgctcgaaat gactcgcgta tccctcggac ttgcagacga tttcgccgac ggaatcatcg
     3061 ccaaacgaat cgtcgaccgt gccaaggcgg gcgagcgcaa tcccgatctc ttgtgtcaag
     3121 gcgcgctcga tgaattacgc gggcatttgt acggagacta acaagcaaaa aacgccccga
     3181 ttgccacgac ctaccgggcg gctggtccgc gaggtcgccc tgcgccgccg caagaccaca
     3241 accaagcgca gccagcgccg agagcattac cagctcccgg cgcaaatcgg ccgtccaaaa
     3301 aataatgggc tattcgaaga gtgacctgtg acagcggtca cagtagccga ggccgaaagc
     3361 gggcataggt agtttgcaat gcccatgaca gtcttgatat gcggcagccc tggcagtggc
     3421 ttcgagatta tcggcctgtt cgccaacagt ggcgatgctc aatcatatgc cgaagactat
     3481 agcctcaaag attggtgggt tgccgaccta atatcaccgc gggcatttgc ggaaagccaa
     3541 gtggcgacgg cttcgaaggc ccatctctga gtgacttccg aatcgtttat cgaaactcga
     3601 tccactcatg gggccgatgg cttagccgcc tttggtctcg ctgacattga cgggcggtcg
     3661 gacaggctcc gcgcaagtct gcgggtgccg cagatgtatc tcgctcgcag ctccgctgtt
     3721 cgccgcgcgg cgcgctgcct cggcgctgca ggagcagcgc tctgttctat gccggggccg
     3781 acgagaccca gtgcggcgcg ctgatcgagg ccggcgcggc gctcgagcac cacggagcag
     3841 cgctgcacat cggctgctct tacatagttc tccctgtgcg ggcggagaac ttggtagcgg
     3901 gacgtccagc taccatccca gaagcggcgc gctgcacggt cactgctcgg cggctgctct
     3961 tgccgcggca ctgcagaagc agcgcctgcc gcgctgctgt cgcggtgcag gtgcagcggg
     4021 ggttttcttt atacgttcgt gaggatgaaa acgctcgcga cgaagatgtt tacgataatc
     4081 gggtcctgga gggagtgctc ataccaaggg tctttatacg gtttcagtag ctcgtctctc
     4141 ttcgaggggg ccaactcgcc tatccgcgcg ccggtctctt cataatattg ctgcagaaac
     4201 cgaacacgcg cgcgaaagcg ctctttgctg ttgttccgaa aaaaggggcg gcagcgccac
     4261 agggtatagc tcgctagttt ctctgtatcc ttttccgtcc aatcctcgat tttcgagtcc
     4321 aacttcttca agaggagagg atgctcgcca tacagcacaa cggcgcgcgc ctcgtcgtca
     4381 tcgctgcctt tcgagggtac tatatcgacg atccaaaaga acgtgcggct aaacagccac
     4441 aggcttcccc ctagcaggag cccgccgatg atgagcgcca gcccaaagac taaatatgcg
     4501 accataatct ttgcccacca cgcatacgcc gtggagcgag cgacgacgat accgaccgga
     4561 atggcagcga acagaataac ccaattggtg aggcgaaaag ggaaggttaa aagccaatag
     4621 agcttgtgag ccctaaaatc gagcatcccg aacatgaatg cccctctcga atcgctctcc
     4681 agataccact aatgaattga ggcatcaccg gggcatcggg cgcccgctcg cctagacctc
     4741 ggtccctctt tcaaagacca ccccacgcag acggcacggc atctagagcc ggacagaccg
     4801 gacaaacccg gacatttgtc cggcatgtcc ggtttgtccg gcggacgcgg gtgtctgttc
     4861 tgtccgggcg tccgtcggct gcttggtacc gatgtccggg tggccaggtg tttcgacgcg
     4921 gcttctcttg gcagatgtca cgttcggctt ggccgctggc cagccatgtc catgtgaggt
     4981 gtccaatatc gcggatgacg ataacgccgc ttgcttgtgc gtcgttgatg gctcgccgga
     5041 atgctttctg cagtgcagct tgcttctggg tggaatcgcc gtcggcaggg tagacggatc
     5101 ctccgaccgc atccatgggt gactccgccg tccacctcag cggacacgtc gacgccgccg
     5161 ccgcacgctt gatgcttgac cgcgcactca ttgacccagc tcaatctcta gggcatcagc
     5221 gcgggcgcgt gagcaggaag aatcgccgac gcccgctcgc cacaacgttc aacgccaccc
     5281 tttttgaacg tgaagactgc acgctcgtgg cgtggtatcg ttccccttac gcacctcgcg
     5341 cgggaggagc gtatgacagt caccatcgga cggcgggaat tgctggccgc acttggcggc
     5401 gcggcggccg cctggccgct cgcggcgcgc gcgcagcaga cgcacttaat tacatccccc
     5461 cgcgtcgcgt tcctcggcgc tgaatctggg tctaccaacc agcacttctt cgacgcattc
     5521 cgccaaggga tgcacgagca tggttacgtc gacggtcaga acattacttt tgtggagcga
     5581 tgggctgaag gccggagcga gcgctttcct gaaatcattg aagagctgat cagcctcaag
     5641 gccgatgtta ttttggcgct cagtctacct gcggctcagg ccgcgaagag cgcgaccaca
     5701 acggttccaa ttgtcttcat cgcgagcgat ccgttgggat cggggctcgt cagcagtctg
     5761 gcccggccag ccggtaatct caccgggttt tcgctcgccc tcggtgacga attcagcagc
     5821 aagtggcttg aactcctgaa agaggcggtt cccagcatct ctcgtgtagc ggtactttgg
     5881 aacccagtga atcctgccag ttctcactac gtgactgtcc ttcgagatgc ggcagaaaaa
     5941 ctaggggtca gggtccaacc tcaggcggta agcgacccag accagtttaa cggcgccttc
     6001 gcaaccatag ttgcagagcg tgcgcaggcg ctcatcgtgg tggttgaccc cgtgaccgct
     6061 cggtatcggg agcgtgtcgt tgagcttgcg atgaaaagcc gactgccggc gatgtatggt
     6121 ttccgcgagt ttgtagacgc ggggggtctc atcgc
//
LOCUS       DATLJT010000379         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1859455,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000379 DATLJT010000000
VERSION     DATLJT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859455"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VM910_08740"
     CDS             complement(<1..611)
                     /locus_tag="VM910_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62657.1"
                     /translation="MPAISRCAWAQAYPTRPVRIIVGFPPGGPSDILARLIGQWLSER
                     LGQPFIIENRPGATGNIGTEAVVRAPADGHTLLLVVPSNGIAHVLYDRLNFNFIRDTA
                     PVAGISNGPLVMEVNPTVPAQTVPEFIAYAKTNPGKINFASPGNGSTIHLCGELFKMM
                     TGVNMVHVPYRGNAPALTDLLAGQVQMMFADTPSSIEHLRAGK"
     gene            complement(699..1688)
                     /locus_tag="VM910_08745"
     CDS             complement(699..1688)
                     /locus_tag="VM910_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62658.1"
                     /translation="MKLPRRNFLRLAAGAAALPAMSRAATAQDYPTRPITIVVPFPAG
                     GPTDALARVLAERMKAALGQYVIVENPTGAAGTIGTGRVARATPDGYTVILGHWQTHV
                     VNGATYALPFDVVKDFEPISLIADCPVWFVAKAALPPKDLRKLIAWMKENPGKATVGI
                     GGVGGGADVVGTYFQNNTGTRFQFVPYRGAAPMIQDLVAGQIDLTFTQVASALAQVRA
                     GQLKAYAVMAKTRWAAAPDTPTFDEAGVPGLHASFWHGLWAPKGTPKDVIASLHAALV
                     ETLADPSVRQRLTDLGQGLWPRDKQTPEALAAQQKAEIEKWWPIIKAANIKAE"
     gene            complement(1707..2702)
                     /locus_tag="VM910_08750"
     CDS             complement(1707..2702)
                     /locus_tag="VM910_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62659.1"
                     /translation="MTVTIGRRELLAALGGAAAAWPLAARAQQQRLPVIGFVNSSSPH
                     LYVPFIRAWQQGLGETGYAEGRNVAVEYRWAMGHYDRLPALVTELAGLPVAVLAATSG
                     PAALAAKAANTTVPIVFTMAADPVELGLVASLARPGANITGVTQMTTEVGPKRLELMH
                     ELLPAAATVALLVNPNNSVIAETEQRNLRATARTLGRQLIVLNAGSEHDLDKVFASLV
                     ELRAGGLLISTDPFFFGQSARLADLAQRHAMPTLSVDREFVAAGGLISYGGDVAHSYR
                     LAGVYTGRILKGDKPADLPVQQSTKVELHINLKTAKALGLTVPLPLLGRADGVIE"
BASE COUNT          532 a          948 c          950 g          534 t
ORIGIN      
        1 aatttgccgg ccctaaggtg ctcgatcgac gacggcgtgt cggcaaacat catctgcacc
       61 tgcccggcga gtagatcggt caacgccgga gcattgcctc gatacggcac gtgaaccatg
      121 ttcacaccgg tcatcatctt gaacagctcg ccgcacaaat ggatcgtgga cccattgcca
      181 ggcgacgcaa aattgatctt gcccggattg gtcttggcgt aggcaataaa ctcgggaacc
      241 gtctgggccg gaaccgttgg attgacctcc atgacaagag gcccattgct tatgcccgcg
      301 accggcgcag tgtctcggat gaaattgaaa ttcagcctat cgtaaagcac gtgggcgatc
      361 ccgttcgagg gaactaccaa aagcagcgta tggccgtcgg cgggcgcacg cacgaccgcc
      421 tcggtgccga tgttgcccgt agcgcccggc cggttctcaa tgatgaatgg ctggccgagg
      481 cgctcggaca gccactgacc gatcaggcgc gcgaggatgt cggacgggcc gccgggcgga
      541 aagccgacga tgatgcgaac cggccgcgtc ggataggctt gtgcccaagc gcagcgcgag
      601 atagccggca gcgcggcggc gcccgctgcg agatggagaa aatttcggcg gggaagtttc
      661 acggcatcgc ttcctaacaa catcggcaac gaaccgggtt attccgcctt gatgttcgcc
      721 gccttaatga tcggccacca tttctcgatc tcggccttct gctgcgcggc gagcgcctcc
      781 ggcgtctgtt tgtcgcgcgg ccacagtccc tgacccaagt cggtgagccg ctggcgcacc
      841 gatggatcgg ccaaggtctc gacgagcgcg gcgtgaaggc tggcgatgac atccttgggc
      901 gtgcccttgg gcgcccacag gccgtgccag aacgacgcgt gcagccccgg cacccccgct
      961 tcatcgaatg tcggagtatc gggcgctgct gcccatcggg tcttggccat gaccgcatag
     1021 gccttgagct gaccggcgcg tacttgcgcc agcgcgctcg ccacctgcgt gaatgtcagg
     1081 tcgatctgcc cggccaccaa atcctggatc atgggcgcgg cgccgcggta gggcacgaac
     1141 tggaaacggg tgccggtatt gttctgaaag taagtcccga cgacgtccgc tccgccgccg
     1201 acgccgccga tcccgaccgt cgctttgccc gggttttcct tcatccaagc gattagtttc
     1261 cgcagatcct ttggcggcaa agcagctttt gcaacgaacc acaccggaca gtccgcgatc
     1321 agcgatatcg gctcaaagtc cttcaccacg tcgaaaggga gcgcgtaggt ggcgccgttg
     1381 acgacgtgcg tctgccagtg gccgaggatg accgtatagc catccggggt ggcacgcgcg
     1441 acgcggccgg tgccgatggt gcctgcggcc ccggtcgggt tttcgacgat gacgtattgg
     1501 ccgagcgccg ccttcatccg ctcggccagc acgcgcgcca gcgcgtcggt gggcccgccc
     1561 gccgggaagg gcacgacaat ggtgatggga cgcgtcggat aatcttgcgc ggttgcggcg
     1621 cgcgacatgg ccggtaacgc ggccgcgccc gctgccagac ggagaaaatt tcggcgggga
     1681 agtttcattt tgaacctccc ttcacatcat tcgatcaccc cgtcggcacg gccaagcagc
     1741 ggcagtggca cagtaagacc gagcgctttc gcggtcttga ggttgatgtg cagctcgacc
     1801 ttcgtggatt gctggaccgg caagtcggcc ggcttgtcgc ccttcaggat gcgcccggtg
     1861 tagacgccgg ctaaacggta ggaatgggcg acgtcaccgc cgtagcttat caggccgccg
     1921 gcggcaacaa actcgcgatc aaccgagagc gtaggcatcg cgtggcgctg cgccaaatca
     1981 gcgagccggg cactctggcc aaaaaagaat ggatcggtgc taattaggag cccgcccgcg
     2041 cgcagctcga ctaggctggc gaacacttta tctaaatcgt gctcgctgcc ggcattgagg
     2101 actataagct gccgacccag ggtacgcgcg gtcgcccgca ggtttctttg ttcggtttcg
     2161 gcaatgacgg aattattggg attgacgagc agggcgacgg tggcggcagc aggcagcagc
     2221 tcgtgcatca gttcgagcct cttcggtcct acctctgtcg tcatttgggt gacgcccgtg
     2281 atgttagctc ccggccgcgc gaggctagcg accaagccca gttcgaccgg gtcggcggcc
     2341 atggtgaaga cgatcggaac cgttgtgtta gccgccttgg ctgccagtgc cgccggaccg
     2401 ctcgttgcgg cgagcaccgc cactggaaga cccgccagct ccgtgaccag cgctggcaac
     2461 cggtcgtagt ggcccatcgc ccaacggtat tcgaccgcca cgtttcggcc ctcggcatat
     2521 ccggtctcgc cgaggccctg ctgccatgca cgtatgaagg gcacgtacaa gtgcggcgac
     2581 gagctattga cgaacccaat cactggcagc ctctgctgtt gggcgcgtgc cgcgagcggc
     2641 cacgcggccg ccgcgccgcc gagtgcagcc agcaattccc gccgtccgat ggtgactgtc
     2701 atacgcccct cccgcgtgag gtgcgtaaag ggaacgatag cacgccgcga gcgtacagtc
     2761 ttgccgttcg aggagggcag gacgctggtt gcttccgcct ctgtcgtcgg cttcaactgc
     2821 accactcccg ccgccagctc ttcgcgaacg ccgccatcgc ggcctcgcgc gtcccgtgtc
     2881 cgcttcgctc cgattgcagc gcgaggcgga caaacgatag caacacgcta tgcaatgaca
     2941 gtcggactgc gggcgcggtt gcgc
//
LOCUS       DATLJT010000380         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1859473,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000380 DATLJT010000000
VERSION     DATLJT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859473"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VM910_08755"
     CDS             <1..209
                     /locus_tag="VM910_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62660.1"
                     /translation="AAKYLNGHFGGLFVYFLHFALKTSKRTLDDPYDLAFAHLMMFSH
                     TAIRFLYGLRLPGTGKLGSIISAR"
     gene            complement(1277..2287)
                     /locus_tag="VM910_08760"
     CDS             complement(1277..2287)
                     /locus_tag="VM910_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62661.1"
                     /translation="MDTLFGEGQSGLKVVLFVIVVLGLLALAFWLLRWSGGRRLGSGA
                     TRGHRLAVIDQATVDRRRRLVLIRRDNVEHLLIIGGPRDVVVEQNIVRAATALRETGP
                     ARPPATPRVPLGEDSIWPLQPEPAPPGAPAPRKPRTASRAPEVGEPAEQRRPPRLQPA
                     PPARSTADAAFKPATDQNLSEMAQRLEATLRRPTKSSADARTAAGNPKTTAEQSAPEP
                     FMAAPPFARLDTMQRRPFRTDEPRAPSGAPKAPSEPAAPPREPDTRGRGNDKAPSEPA
                     APPREPDTRGRVDDKAPSEPAAPPREPDTQGRGDDKAPSEPAAPPREPGTQGRRDDGP
                     SS"
     gene            complement(2609..>2928)
                     /locus_tag="VM910_08765"
     CDS             complement(2609..>2928)
                     /locus_tag="VM910_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62662.1"
                     /translation="VKVGVQGRVILGPMGGPGKVEVPLRYAVVREGPQPKTVITKFKR
                     IAITVAPGQTHVQFVDIEEGLGFPMPSRSDLDAYVVYVGFDEIGDKNEKKPSAKTRAT
                     RQQ"
BASE COUNT          494 a          916 c          862 g          656 t
ORIGIN      
        1 atgcggccaa atacctgaac ggccactttg gcggtctgtt cgtctacttc cttcacttcg
       61 ccctgaaaac cagtaaacgg accctcgatg acccgtacga tctggccttc gcgcacctga
      121 tgatgttttc tcatacggcc atccgctttt tgtacggcct gcgcttgccg ggcaccggca
      181 agctcggttc gatcatttcg gcaaggtagc gggccgaaag gccgtcctag agagacatca
      241 ttaaagttcg ggcacgtcgc gttatcaagc ccgattaatg agtacacgcc ctagtctcct
      301 cattcaaact tataacgact gagatttgat tgttgtggcg atagcagagc cagtagaggg
      361 gcatgactga cctgttgtcg gtcccattcc attctacaaa ttgtcctaat ctgatgggag
      421 ccttggcccc agtctgggag gtttgggatg tggtgggcga agctcggcct tgcgttgatg
      481 gccctgggga tgatgtcgac ggtccccgtc agagtggcgg atgagatcgg ggcgactgcc
      541 cgtctcatta cttggtgtag tcaacctgat ggtacgcacg gtagaggctt ctgccttgca
      601 tacatccatg cgattgctca aagggtcgag gatgtctgtt tacccaatcg gcctgatcag
      661 gatcggatca gcgtgttgat gttcctgtca ttcactgaca gacatcctga attgctgcac
      721 aagcatccaa gtgtcttgat cagtcaggca atcgcagagg cgtttccctg tcgctcatga
      781 gacggtttta gacacgttaa ttcgtgagat gtggataggc ccatacctat aactccccac
      841 aaaatttgcg aaggtgtacg aagctcagcg cttcgatcta ccctttttcc gtcccttgcg
      901 tgctgacttg tcaatcagaa aatcctccag cttctttcca cctttgatcg cagcaacaag
      961 ccacgttggc tttgccccac gacctgccca agtctcgcct gagggactac gatatttcgg
     1021 cgggaccttc tttcctctta atgggcttcc acctcctcgg acaacccttg tgccgccaac
     1081 cacggcaatg ctctccaact gcttttcaag ctcggctcga cgcctgtgaa gcatttcgtc
     1141 aacccgcttt cgcaggtcca tgagtgcttc gacggtcatc cctgatagat ttatgtttgc
     1201 catagtgatt tccggttttc gcgaagatcc gccggcacct tcaactgtcg gttaaagcat
     1261 taaaggcggg gctctattag gaactggggc cgtcatcgcg acggccctgc gtgcccggtt
     1321 ctcgcggcgg cgctgcgggc tcgcttgggg ccttgtcatc gccacggccc tgcgtgtccg
     1381 gttctcgcgg cggcgctgcg ggctcgcttg gggccttgtc atcgacacgg ccccgcgtgt
     1441 ccggttctcg cggcggcgct gcgggctcgc ttggggcctt gtcattgcca cggccccgcg
     1501 tgtccggttc tcgcggcggc gctgcgggct cgcttggggc cttgggcgct cccgacggcg
     1561 cgcggggctc gtccgttcta aacgggcggc gctgcatcgt gtctagccga gcgaagggcg
     1621 gcgcagccat gaatggttcg ggcgcagact gctcggctgt cgtcttcggg ttccccgcgg
     1681 ccgtgcgggc gtcggcgctg ctcttcgtgg ggcggcgtaa tgtggcctcg agccgctgtg
     1741 ccatctcgga gaggttctgg tcggtcgctg gcttgaacgc cgcatcggcc gtgctcctgg
     1801 ccggcggcgc cggttgcagc cgcggcggac ggcgttgctc ggcaggctcg ccaacctccg
     1861 gcgctcgcga ggccgtgcgc ggcttgcgcg gcgccggagc acccggaggc gccggttcgg
     1921 gctggagcgg ccagatgctg tcttcgccga gggggacccg tggcgtggcg ggcggacgtg
     1981 cggggcctgt ttcccgaagc gcggttgccg cgcgcacgat gttctgctcc accaccacat
     2041 ccctcggacc gccgatgatg agcagatgct ccacattgtc gcggcgaatg aggacgagac
     2101 gccgtcgcct gtcgaccgtt gcctggtcga tgacggccaa tcggtgtccg cgcgtcgcgc
     2161 cgctgccgag ccgcctaccg ccggaccagc gcaacagcca gaacgccagc gcgagcaggc
     2221 ccagcaccac gatcacgaac aaaacgactt tgaggccgct ctgtccttcg ccgaataagg
     2281 tgtccatcac ttctccccgc atctcacggg tgcggcgcct caatggggat cgcccgaatg
     2341 gttaacgccg ctgctactgc tcgccgtcaa tactaacagg aaatgaagaa aatcgttgga
     2401 cgtcttatcg cattcctcgc actggctgtg ctcgcaaacc ccaacgccag aacttttttg
     2461 ccagtcctga ggtccttggg gcagacttga ggctccctgc cgtcatcgcg cgcatcgcct
     2521 acatcacggc caaggtcgcg ctgggtcgag tcacccatcc tcttaggcct gcgcgatctg
     2581 accatcatcg cagtgggcaa gagaacctct attgctgccg tgttgccctc gtcttcgcgg
     2641 acggcttctt ctcgttctta tcaccaatct cgtcaaaccc gacatacacg acataagcgt
     2701 cgaggtccga ccgcgacggc atcggaaagc ccagtccttc ctcgatatcg acgaattgga
     2761 catgggtctg gcctggcgca acggttatcg caatccgttt gaatttggtt atgacggtct
     2821 tgggctgcgg accctcgcgc accactgcat agcgcaaggg gacctcgacc ttccccggac
     2881 cgcccatcgg tccgagtatg acgcggcctt gaacgccgac cttcacga
//
LOCUS       DATLJT010000381         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_21842,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000381 DATLJT010000000
VERSION     DATLJT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21842"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VM910_08770"
     CDS             complement(<1..306)
                     /locus_tag="VM910_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVI62663.1"
                     /translation="MSLVVDAHQHFWTYGTYQTSWMDVPPYAGDPAFQTLRRSFHPDD
                     LMPELKAAGVRCTVTIEAADNLAENEALLANTRTYDWIAGVVGWAPLAHPSDVERTLD
                     "
     gene            complement(445..1434)
                     /locus_tag="VM910_08775"
     CDS             complement(445..1434)
                     /locus_tag="VM910_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62664.1"
                     /translation="MKRRDFITLLGSAAAAWPLAARAQQGERIRRIGVLQAISESDPE
                     AHLRKAAFVGGLQKLGWTEGTHVTIDYRWAGADADRIRLYATEITGMRPDVIWTSGAL
                     PLLPLKRATRTIPIVFTQVYDPVGSGFVASLTRPGGNITGFTLGEFSMGGKILEVLKE
                     VAPQVNRVAVILNLEQPPHVAMWRSIEATAPSFGVRLTPADVQGPAEIERAMEGLAHE
                     PNGGLIVLPGPITIAHRELITALAARHRLPAAYAFRFFVTGGGLLSYGIDSADQSRQA
                     AGYVDRILKGEKPGDLPVQQPTKFELVINLKTAKALGIEVPPTLLARADEVIE"
     gene            complement(1906..>2091)
                     /locus_tag="VM910_08780"
     CDS             complement(1906..>2091)
                     /locus_tag="VM910_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62665.1"
                     /translation="TGQAEAAQAMSDYAYAALRDPREIWLCGWRSATALTPSISAVDL
                     GRSQLTHLLSLADEVIG"
BASE COUNT          417 a          678 c          605 g          391 t
ORIGIN      
        1 atcgagtgtt cgttcaacgt cgctcggatg cgcgagcggc gcccaaccaa ccacgcctgc
       61 gatccaatca taggtgcgcg tattggcgag caatgcctcg ttttccgcta ggttatccgc
      121 cgcttcaatc gtcacggtgc agcggacccc agctgccttg agctcaggca tcaggtcgtc
      181 cggatgaaag gaacgccgaa gcgtttggaa cgcgggatcg cctgcgtaag gcggtacgtc
      241 catccaggac gtttgatagg taccgtaggt ccagaagtgc tgatgtgcat cgacgacgag
      301 actcatcgaa tgcctccgag agtccggaac gcccctcgcg gcctgtgtgt gccgaaaatc
      361 agattcttat tggtgatgga gaccgcgcca cgagtggcca cgccgccgcc gcgccgccga
      421 gcagcgtgat gaacgcgcgg cgcttcactc aatcacctcg tcggcgcggg cgagcaaagt
      481 cggcggcacc tcgatgccga gcgccttggc ggtcttgagg ttgatcacga gttcaaactt
      541 ggtcggttgc tgaaccggaa ggtcgccggg cttctccccc ttgaggatgc gatcgacgta
      601 gcccgccgcc tgccgggact ggtcagcgga gtcgatgcca tacgacaaca agccgccgcc
      661 cgtgacgaaa aaccgaaacg cgtaagcagc cggaaggcga tgccgggccg ccagcgcggt
      721 aatcagttcg cgatgcgcga tcgtgatcgg ccccggtagc acgatcagtc ctccgttcgg
      781 ctcatgcgcg aggccttcca tcgcacgctc gatctcggca ggaccttgca cgtcggccgg
      841 cgtcaggcgc accccgaacg acggcgcagt cgcctcgatc gagcgccaca tcgcgacgtg
      901 aggcggctgc tcaagattca aaatgaccgc tacacgattc acctggggtg ccacctcctt
      961 cagcacctcg aggatcttcc cgcccataga gaactcgccc agcgtgaatc cagtgatgtt
     1021 gccgccgggt cgagtgaggc tcgcgacgaa cccgctgccg accggatcgt agacctgcgt
     1081 gaagacgatc ggaatggtgc gagtcgcccg ctttaacggc agcaacggca gtgcgccgct
     1141 ggtccagatc acgtctggcc gcatgccggt aatttcggtc gcgtaaagcc gaatgcggtc
     1201 agcgtcggcg cctgcccacc gatagtcgat cgttacatgg gtgccctctg tccagccgag
     1261 cttttgcagg ccgcccacga atgccgcttt acgaaggtgc gcttccggat cgctctcact
     1321 gatcgcctgg agcacgccga tccgcctgat ccgctcgccc tgctgcgcgc gcgccgccag
     1381 cggccacgca gccgccgcac taccgagcag cgtgatgaag tcccgccgtt tcacagtacc
     1441 tcccgtgctt ccgaccgaaa ggatagcaca cctgagcacc gcacgagact gctgcgctgc
     1501 aagatttcaa tccgccctat cccgggtgtg ggtcattttc gtcagatcgg gacgctccga
     1561 acgctcacga gatgtccgct tcgctccgaa agcggacatg cacacgcttg cctcgaaatg
     1621 tccgcttact gccgatactg ttgcaaaagt tcaaaattgc ccggtgataa attttccgcc
     1681 gtaagacaat ccaaccgacg accgccgatc tgtgtctctc aatcacgtta ccgaggtcac
     1741 ccgagcgttt atcgtcaggc gatgaggtcc cccacatctt tacacgaaag ccgtgtctac
     1801 agcccgcaga atttttgatc atcagtgcaa aaagactttt gcaacagtat ctgccagaag
     1861 ccgactcctg cactgcagca aacaacatgc atgggctgca acgccttacc caatcacctc
     1921 gtcggcgagc gagagtagat gcgtcaattg ggatcgcccg aggtcaaccg ccgaaattga
     1981 cggcgtgagt gcggttgcgg atcgccaccc acaaagccag atctcgcgtg ggtctcgcag
     2041 tgcggcgtaa gcataatcgg acattgcctg cgccgcctcc gcctgtccgg t
//
LOCUS       DATLJT010000382         5114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1630035,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000382 DATLJT010000000
VERSION     DATLJT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630035"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VM910_08785"
     CDS             <1..1153
                     /locus_tag="VM910_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HVI62666.1"
                     /translation="QTMVNGTGNFILLAVPFFILAGLIMERGGISVRLVRFIYTLVGH
                     WRGGLLQVTVASMYVVSGLSGSKPADVAAVGTVMRDQLRERHGAAEGAAVLAASAIMG
                     ETVPPSIAMLIVGSITSVSLAALFIGGLIPAAVMAFCLMVLIYVRARRAGTLRVTRAS
                     FAAMFRAGLDAVLPLLMPAMLLGGILFGVATPTEVAAFAVVYGLVLAIIVYRVMNFGE
                     FMRTVADTAALTGVLLFIFAAASGFSWTLTVAYLPQRLVALLHAVGNSTEIFMLGSIA
                     LLVVVGVLLEGLPSLNVLAPLLIPIAGKLGVSELHYALVLIIAMGVGGFMPLAGVGFY
                     VCCAIMRCDIEQASRAMLPYLIVVLTGLIIVAFVPWFALALPKYFGFRL"
     gene            1196..2206
                     /locus_tag="VM910_08790"
     CDS             1196..2206
                     /locus_tag="VM910_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62667.1"
                     /translation="MDRRMFLKASAGGAALTLSAPFVALHAAPLTLRWAHFAQEDHPA
                     NTAAKQFASRVEARTGGEIKINIFPNNVLGGPPEQAQQIKLGTIDMGLPTQGQLDKYD
                     TAFAAVMLPFVFDGPEHVFRVLDGPAMAWLAPLAEKQGFILLHNWEYGFRNVTNSVRP
                     IHTPDDVKGLKLRTPPELQIQASMEALGAVVQAIAFPELYLALSQKVVDGEENPIAVI
                     YFNKFYEVQKHLAITRHVYNNMIHTVSAATWKKLTPAQQAIFREESAAAGNRMRKLIA
                     DDEADQIKKLEAAGMLVTRPDLAPFRAKMEPAYARIASYAGEANVKKFRELVEQGRNA
                     AR"
     gene            2254..3057
                     /locus_tag="VM910_08795"
     CDS             2254..3057
                     /locus_tag="VM910_08795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI62668.1"
                     /translation="MFRLDDKTALVLGAASGIGKASAEALAALGAIVICADKNKEGVE
                     TTAADIGLRSPTEAHVVDANSAESVNALAAAIETNHKRLDIAVTTPAVHVRKLMCDYT
                     DDEYDRVADLNLRGTFFFLRAFGRMMVRQRGGSLIASSSVRATTLEPGLAIYGATKAG
                     IIQMVRGLASEIGPSGVRVNAVVPSIVETPLVAPLKARTEIWNALASHTVFNRWAHPS
                     EVAAAVAFLASDAASYVTGTALLVDGGWTAIDGPPTGLTTTHQKSATTP"
     gene            3760..4242
                     /locus_tag="VM910_08800"
     CDS             3760..4242
                     /locus_tag="VM910_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVI62669.1"
                     /translation="MDILDIAVRLGAATLVGVLLGLNRDLHGKQTGVRTLGLVCLGSA
                     LAVLSIHAATGTEESRVIQGIVTGVGFLGAGVIVRSEKGQHVHGLTTAACVWVTACVG
                     AACAIAQWQIVVVGVVLVLAILVFGGPFEKAIDRRWPGRPSGPSDQPPDRTRAPGPEM
                     "
BASE COUNT          895 a         1573 c         1611 g         1035 t
ORIGIN      
        1 gcaaaccatg gtcaacggga ccggcaattt cattcttctg gccgtgccgt tcttcatcct
       61 ggccgggctg atcatggagc gcggtggcat cagcgtgcgg ctcgtccggt tcatttacac
      121 gctggtcggc cactggcgcg gcgggctgct ccaagtcacc gtggcgagca tgtacgtcgt
      181 ttccggcttg tcgggttcca aaccggcgga cgtggcggcc gtcggcaccg tgatgcgcga
      241 ccagttgcgc gagcgccatg gtgccgcgga gggcgcggcc gtgctggccg cttcggcgat
      301 catgggtgag acggttccgc ccagcattgc catgctgatc gtcgggtcga tcaccagcgt
      361 atcgcttgct gccctgttca ttggcggctt gattccagcc gccgtcatgg cgttctgcct
      421 aatggtgctc atctatgtgc gcgcccggcg cgccggcacg ctgcgcgtga cgcgcgcgtc
      481 ctttgccgcc atgttccgcg ctgggctcga tgcggtgctg ccgctgctga tgcctgccat
      541 gctgctcggc gggattctat tcggcgtcgc aaccccgacc gaggtcgcgg ctttcgcggt
      601 agtctatggg ctcgtgctcg cgatcattgt ttatcgcgtg atgaacttcg gagagttcat
      661 gcgcacggtc gcagacacgg ccgcgctgac cggggtgctc ctctttatct ttgccgcggc
      721 atcggggttt tcatggacac tgacggtggc gtatttgccg cagcgtctcg tggcgctcct
      781 gcatgcggtc ggcaatagta ccgagatctt catgctcggc tcgatcgcgc tcctcgtcgt
      841 tgtcggcgtg ctgctcgagg ggctaccatc actcaatgtg ctcgcgccgc tgctcatacc
      901 gattgccggc aagctcggcg tgagcgaact gcattacgcc ctcgtcctga tcatcgccat
      961 gggcgtcggc ggcttcatgc cgctcgcggg ggtgggtttt tacgtctgtt gtgccatcat
     1021 gcgctgcgac atcgagcaag cctcacgcgc catgctcccg tatctgatcg tggtgctcac
     1081 cgggctcatc atcgtcgcgt tcgtgccgtg gttcgcgctc gcgctgccga agtatttcgg
     1141 cttccgtttg taacgtcgtg ccgctgtcat gcggacaaca tcaagggaga actccatgga
     1201 ccgcagaatg tttctcaagg ccagcgccgg cggcgccgca ctgacgttgt cggcgccgtt
     1261 tgtggcgctc catgccgcac cgctaaccct gcgctgggcg catttcgccc aggaggacca
     1321 cccggcgaac acggcggcca agcagtttgc gagccgcgtc gaggcccgca ccggtggcga
     1381 gatcaaaatt aacatttttc caaacaacgt gctgggaggc ccgcccgagc aggcgcagca
     1441 gataaagctt ggcaccatcg acatgggcct gccgacgcaa gggcagctgg acaaatacga
     1501 caccgcgttc gccgccgtga tgttgccgtt cgtgttcgac ggcccggagc acgttttccg
     1561 cgtgctcgac gggccggcga tggcgtggct cgcgccgctt gccgaaaagc agggctttat
     1621 ccttttgcat aattgggaat atgggtttcg caacgtgacc aacagcgtcc gcccgattca
     1681 tacgcccgat gacgtcaagg gcttgaagct gcgcacgccg ccggagctac agatccaagc
     1741 atcgatggag gcgctcggcg cggtggtgca ggccatcgct ttcccggagc tctatctggc
     1801 gctgtcgcag aaggtcgtgg acggcgagga gaatccaatc gcagtgatct acttcaacaa
     1861 gttctacgaa gtgcagaagc atctggcgat cacgcggcac gtctacaaca acatgatcca
     1921 cacggtgagc gcggcgacct ggaagaagct gacgccggcc cagcaggcga tcttccgcga
     1981 ggagagcgcc gctgccggca atcgtatgcg caagctcatc gccgatgatg aagcggacca
     2041 gatcaagaag ctcgaagccg cgggcatgct agtgacgcgg ccggacttgg cgccgttccg
     2101 tgccaagatg gagcctgcct acgcgcgtat tgccagttat gccggcgagg ctaacgtgaa
     2161 gaaattccgc gagctggttg agcagggccg gaacgcggcg cgatgattgc agcgaacggc
     2221 ccgatcgatg aacggctgga ggcttatcgg gccatgttta ggctcgatga caagacagca
     2281 ctcgttctgg gtgccgcctc tggcattggc aaggcttccg ccgaggcgtt ggcggccctc
     2341 ggagcgattg tcatttgcgc cgacaagaac aaagaaggcg tagagaccac ggcggcggac
     2401 atcggcttac gaagccctac cgaagcccat gtcgtggatg ccaatagcgc ggaatccgtg
     2461 aacgcgctcg cggcggcgat cgagacaaac cacaagcggc tcgacatcgc cgtgaccacg
     2521 cccgcggtcc acgtcaggaa gctaatgtgt gactacacag acgatgaata tgaccgcgtc
     2581 gccgacctca atcttcgagg gacgttcttt ttcctgcgcg ctttcggccg catgatggtg
     2641 cgccaacgcg gcggaagctt gatcgcgagt tcgtcggttc gcgcgacgac gcttgaaccg
     2701 ggtttggcca tctacggcgc aaccaaggca gggatcatcc aaatggtgcg cggccttgct
     2761 tccgaaattg gtccctctgg ggtacgggtc aatgccgttg ttccgagtat cgtggagacc
     2821 ccacttgtcg ctccgctgaa ggccaggacg gagatctgga atgctttggc gtcacacact
     2881 gtattcaatc ggtgggccca tccgagtgaa gtggcggcgg cagtcgcttt tctcgcctcg
     2941 gatgccgcga gctacgtaac cggcaccgcg ctcctggtag atggcggctg gacggcgatc
     3001 gatggaccac ccacgggact aacaacaacg catcagaaga gcgcgaccac gccgtgatcg
     3061 cgacaaggcg aagcggcgcg tgtgcagccg ccgccaacgc gcgtggatat gcggactgct
     3121 gcggctggat gtccatcgct gtctataaac gtttattgga cagttgttgg acactaaccg
     3181 agcgacttag aataaccatt tgattttatt ggcgctccct aggggactac gctgagcaag
     3241 cgagttcaat cacttagcga aaagtgggac acccaatcgt tccacggaat cattaggctt
     3301 tttgcctcct gtgtcccacc tggggcgcgc cgttcaatta ctcgcctccg aggatgttgc
     3361 cccagcgcga ccgtgtggct gccgataact gtctttctgg cgggacatga ggatcagttg
     3421 gtctgcccta tccatccgcc cccacgcgct cagaccacgc cgttgctcct gcaacgcgcc
     3481 ggccgccgcg agcgccaccg cggatgccag cgccgcgacc tggcgacgca ccgcggcggc
     3541 ccgacggtgg gcgctccgcg cgcttttggg ttcggaccag ccgccgggcg acctctctag
     3601 cccgtagatt caggtctgcc caaggacttg cctccaccct gcggccggtt ggaggttcgg
     3661 aagactgagg acgggccact cggcggcgct gacggccagg gggaaactcg acccgggcag
     3721 tctgtagatt gccattcgtg gtgctaaaat tgtgccgtta tggacatcct cgacatcgcg
     3781 gttcggctcg gcgcggcgac cctggtcggc gtcctgctcg ggctcaaccg cgatctgcac
     3841 ggcaagcaga ccggggtgcg caccctcggc ctcgtgtgcc tcggctcggc cttggccgtt
     3901 ctgtcgatcc acgcggcgac cggcaccgag gagagccgtg tgatccaggg catcgtgacg
     3961 ggcgtcgggt ttctcggcgc gggtgtgatt gtccgaagcg agaaaggcca gcacgtgcac
     4021 gggctcacca cggcggcctg cgtctgggtg accgcgtgcg tgggcgcggc ctgcgcgatt
     4081 gcgcaatggc agatcgtcgt ggtcggggtt gtgctggtgc tcgccattct cgtgttcggc
     4141 gggccgttcg agaaagcaat cgaccggcgc tggccggggc gaccgagcgg gccttcggat
     4201 cagccgcccg acagaacgcg tgcgcctggc ccggagatgt aaagaaatct tcctctttca
     4261 cggtagcgtc attaagaagc gtgacattcc ctagcctccc aatgcgtttt caacacctca
     4321 attgaagata ttggcttgac ttacggcatc ccaacgcgcc gtgcccgtat ccaccgacca
     4381 ccgttgcgta tgcgcagtgg gacgaagcgc aagcgctcta cggcattttg gaagcagccg
     4441 cagcggcagc gtgatccaac ccccactttt tgccttgcga ccatcggtcg cgcggcgttg
     4501 cgcaccacgc cgaggtgtga agtagctcac acatctctcc cgatttcacc cctcaaagtt
     4561 cggcataacg atatcgccag gaggggacgc atggcaccaa aagctaggct cacatttctc
     4621 ttcgggatgg ttcttacaat ggcgttgacc attctagcgg tcatgacgtg ggcggactct
     4681 gatttggctg cgccgagctg ctcaccgatt taccagcctc aacatttgaa tgtgtactaa
     4741 aaagggccgc cttcaggtcg gggcaatctt tttagaaaca ggcgagccca agcgtgagca
     4801 gtccaatcaa cgggcacgga tgcgctcggt actgcggcga tgctgttgcg acgacggtac
     4861 gtgacgccgg tcgaggcctt ggcatgggtg cctcggcaat atcttcttgc aacgttcgtg
     4921 cgcccgcgcg catcacccgc tgactgatcc tcgccaattc ggtataccct gcgatccggc
     4981 ccttggttgg cagcgatcgg atgcaattcg atccaagatg gtgtcccatc ttggcggtgg
     5041 agaacggatc atgacgtccg atgtccgcgc tctcctgcgc cctctcatga agacgggcgt
     5101 gctgctttta accg
//
LOCUS       DATLJT010000383         5891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3927528,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000383 DATLJT010000000
VERSION     DATLJT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927528"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1054
                     /locus_tag="VM910_08805"
     CDS             77..1054
                     /locus_tag="VM910_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62670.1"
                     /translation="MAGPRFALTLATALVATTLAGAAARAEDYPVRPIRVVVPFGPGG
                     PADVAARLIGNVLQESLGQPFVIENRTGAGGVIGTQEAAKSLPDGYTLLMMSNTQTAN
                     ESLVPLAQRKYELMRDLVPIAPVNYSDLVIVVHPDVAAKTLQDFIALAKAQPGKLNYA
                     SSGQGTPYHMAGELFKTMAGVDIVHVPYRNSGEARSGVIGGQVQMMIDAVPAMAPNIA
                     EKQVRALATTGTARSSVLPDVPTVTEAGVPGYEATIWLGLMAPAGTPKPIIDKLNAAV
                     SAFVKRPEILKLWREQGALPMSMSPEEFDKFLRNDIAKWAEVVKKFDKS"
     gene            1077..1547
                     /locus_tag="VM910_08810"
     CDS             1077..1547
                     /locus_tag="VM910_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI62671.1"
                     /translation="MPVIRFRLNGVATEVDADPNRSLLDILRSRLGLTGTHFGCGSGE
                     CGACHVIVGEHAVSSCDTPLWSVADKDITTVEGLGSAERPHPLQRAFIAEQALQCGYC
                     VPGILMSAAALLKRNARPTEDDARAALDRNLCRCGSHNRMVRAVLRAAEDMASS"
     gene            1544..3667
                     /locus_tag="VM910_08815"
     CDS             1544..3667
                     /locus_tag="VM910_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62672.1"
                     /translation="MSNATPQLPVSLAANPKLSSWVKFAPDGEVAISPGKVEIGQGIV
                     TALAQIAADELDVDIGRIRMVRASTASSPNEGVTSGSLSIQQSGRAIRQACAEVRRIF
                     LGIASDRLGVDPTALTIEDGTISGPGNISTSYWELAEEVSLDRDATPGAEPKPVAQRA
                     LAGRSVERLDIPDKVLGKPRFIHDTALSSMLHGRVLRPERSRAKLDGLTEDVARAVPG
                     FVAVIRDGSFAGVVCETEAAAEAAIAALRKSATWLAGEALPDENNLAAFLKAQPVEST
                     VVGTRTSPNPRRKARTIRSQFTRPYIAHASIAPSCAIAQWNGDRVHVWTHSQGVYLLR
                     TDLALVLKIPVDHITVEHLEGAGCYGHNAADDVALDAALLAKAAGGKPVRVQWSRADE
                     MTHGPFGAAMAIEIEADLDADNEIVAWRHTIWSNGHAARPGRASQPALLAGFELEHPF
                     PRTISSNPPQANGGGADRNSIPLYDFPSWTIESHRLLTMPVRTSALRTLGAQGNVFAI
                     ESFLDEIAAERGEDPLIFRLRHLSDERARDVIRSVAKRANWKPAAKAGTGYGVGFARY
                     KNTGAYCAAIAEIEGDEDIRVRKLTLAVDVGEAINPDGVRNQIEGGAIQATSWVLKER
                     VRFDSERITSNSWTDYPILRFSEVPRVDVELIQRSETDPVGAGEAAHAPVTAAIANAV
                     FDCLGVRVRDLPITRDSLLAAMERG"
     gene            3664..4359
                     /locus_tag="VM910_08820"
     CDS             3664..4359
                     /locus_tag="VM910_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI62673.1"
                     /translation="MMNLDILSGGAAQGLVASLAAAFKAKTGFDIAGEYGAVGSMADK
                     LRDGAPADMIILTSALVAELAEEKLVVSSSISDVGLVETALAVRSGDPKVSATTVDDL
                     RKAFLAADAIFVPDTKASTAGIHVAKVLDRLGIADEVAARLKIHPNGATAMRELAASR
                     ERRPIGCTQSTEIISTKGVELSGSLPKACALATMYTAAVAAKAGHAAQAKALIELLSS
                     AETSEARRRAGFL"
     gene            4546..5232
                     /locus_tag="VM910_08825"
     CDS             4546..5232
                     /locus_tag="VM910_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI62674.1"
                     /translation="MRRNGRPRAATASSRIYADLRVELVALRRHPGEAISEGEIALSY
                     GVSRTPVREAILKLSDEGLVEIFPQSGIIVSRIPLAALPEAIIIRKALEETTTRLAAE
                     LATPSQVLALQSVLQRQREADAARDREAFHQADELFHATIAEIARYPGIWKLILQVKV
                     NVDRFRRLTLPQAGRMAQVIDEHELILSAIEAHDAPRAALAMGKHLERLLRDIAAIQN
                     VNPEFFDEQR"
     gene            5283..>5891
                     /gene="dctP"
                     /locus_tag="VM910_08830"
     CDS             5283..>5891
                     /gene="dctP"
                     /locus_tag="VM910_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HVI62675.1"
                     /translation="MRRRDFLKIGAAMGAAAASLPPLSCASAQSKATFKAADVQPAGY
                     PTVAATESLGKKLEAATGGRLSVQMYPSAQLGGEKETIEQTQIGAIQLLRVSAGALGP
                     IVDDVNVVNMPFLFKNTAHAEKMMDGPIGQDLLDRISANANAGLIALCWMNSGARSIY
                     NTKRAVKSIEDLKGLKIRVIGNPIFIDMMNALGGNGVAMGYDQ"
BASE COUNT         1156 a         1879 c         1859 g          997 t
ORIGIN      
        1 taaacctagt ctatcgtcga cagtcggttt gcgttgcagc taaattgccg ggcaaagaac
       61 ggtgtgggag acgacgatgg ccggcccaag gtttgcgctg acgcttgcga cggcgctggt
      121 tgcgacgacc cttgccggcg cggccgcacg ggccgaagat tatccggtcc gcccgatcag
      181 agtagtcgtg ccgttcggcc ctggcggccc tgccgacgtt gcggctcgcc tgatcggcaa
      241 tgtcctgcag gaaagcctcg ggcagccctt tgtaatcgaa aatcgcacgg gcgccggtgg
      301 cgtcatcggc acgcaggagg cggcaaaatc tcttcccgac ggctacacgc tactcatgat
      361 gtcgaacacc cagaccgcaa acgaatcgct ggtgccgctg gcgcaacgca aatatgaatt
      421 gatgcgcgat ctcgtcccga tcgcgccggt caattattcc gacctggtga tcgtcgtgca
      481 tcccgatgtc gccgccaaaa cgctgcagga tttcatcgcg ctggcgaagg cgcagccggg
      541 aaagctgaac tatgcgtcct cgggccaggg cacgccctat cacatggcgg gcgagctgtt
      601 caaaacgatg gccggggtcg acattgtgca cgtcccctac cgcaacagcg gcgaagcccg
      661 ctcgggcgtg atcgggggac aggtgcagat gatgatcgac gcggtgccgg cgatggcgcc
      721 caacattgcc gagaagcagg tacgcgcact cgccaccacc gggacggcac gttcgagcgt
      781 gctgccggat gtgccgaccg tgaccgaagc cggcgtcccc ggctacgagg cgacgatctg
      841 gctcggcctg atggcgcccg caggaacgcc gaagccgatc atcgacaagc tcaacgccgc
      901 ggtgagcgcc ttcgtcaaac ggcccgaaat cctcaagctg tggcgggaac agggcgcgct
      961 cccgatgtcg atgagcccgg aagaattcga caaattcctg cgcaacgata ttgcgaaatg
     1021 ggctgaagtc gtcaaaaaat tcgacaagtc ctgattaaca tcctccgcag ccccaaatgc
     1081 ctgtcattcg atttcgcctc aatggcgtcg caaccgaggt cgacgccgat cccaatcgat
     1141 cgctgctcga tattctgcgc agccggctcg ggctgacggg aacgcatttc ggttgcggct
     1201 cgggcgaatg cggcgcctgc cacgtcatcg tcggcgagca tgcagtctcc tcctgcgaca
     1261 cgccgctctg gtcggtcgcg gacaaggaca tcaccacggt cgaagggctg ggaagcgccg
     1321 aacgcccgca cccgttgcag cgcgccttca tcgccgaaca agccctgcaa tgcggctatt
     1381 gcgtgccggg catcctgatg agcgccgcgg ctctgctgaa gcgcaatgca aggccgacgg
     1441 aagacgatgc gcgcgccgcg ctcgatcgca atctctgccg ctgcggctcg cataatcgca
     1501 tggtgcgtgc ggtgctgcgc gccgctgaag atatggcgtc ctcatgagca acgctacgcc
     1561 ccaattgccg gtcagcctgg ctgcgaaccc aaagctttcg tcctgggtga aattcgcgcc
     1621 cgatggagaa gtcgcgatct cgccaggcaa ggtggaaatc ggccaaggca tcgtcacggc
     1681 gctggcccag atcgccgccg acgaactcga tgtcgatatt ggacgcatcc gcatggtgcg
     1741 cgcctcgacg gcgtcgagcc cgaacgaggg cgtcacttcg ggcagcctgt caattcagca
     1801 atcgggccgt gcaatccggc aggcttgcgc ggaggtccgg cggatttttc ttggcatcgc
     1861 gtcagatcgg ttaggtgtcg atcccacggc cctcaccatc gaagacggca ccatctccgg
     1921 ccccggcaat atcagtacga gctattggga gctggcggag gaagtttcgc tcgatcgcga
     1981 cgcaaccccc ggagcagaac ccaagccggt cgcgcagcgt gcgctcgccg gccgctccgt
     2041 tgagcggctc gacattcctg acaaggtcct cggaaaaccg cgcttcatcc atgacacggc
     2101 gctgtcgagc atgctgcacg gccgcgtgct gcggcccgag cgttcgcgcg cgaagctcga
     2161 tggtttgacc gaggatgtcg cgcgcgccgt tccaggtttt gtcgctgtga tccgcgacgg
     2221 cagctttgcc ggcgtggttt gcgagaccga agcggctgcg gaggccgcaa tcgccgcact
     2281 gcgcaagagc gcgacctggc tcgccggcga agcattaccc gacgaaaaca atctggcggc
     2341 atttttgaaa gcgcagcccg tggagtcgac ggtcgtcggc accagaacat cgccgaaccc
     2401 ccgccggaag gcgcgcacga tccgcagcca attcacccgc ccctacatcg cacacgcctc
     2461 tatcgcgcca tcctgcgcga tcgcacaatg gaacggcgac cgcgtccatg tctggaccca
     2521 cagtcagggt gtctatctcc tgcgcaccga tcttgcgctc gtcctgaaga ttccggtcga
     2581 ccacatcacc gtcgaacatc tcgagggcgc cggctgctac ggccacaatg ccgccgacga
     2641 tgtcgcgctc gacgcagcgc tgcttgccaa agccgccggc ggcaagccgg tgcgcgtgca
     2701 atggtcgcgt gccgacgaga tgacgcacgg gccgttcggc gcggcgatgg cgatcgagat
     2761 cgaagccgat ctcgacgcgg acaacgaaat cgtcgcatgg cggcacacga tctggagcaa
     2821 cggtcacgcg gcacgtccag ggcgcgcgtc gcaaccggcg ctgctcgccg gattcgaact
     2881 ggaacatccg ttcccgcgca cgatttcgtc caatccgccc caggcgaatg gcggtggggc
     2941 cgatcgcaac tccatcccgc tctacgattt tccgtcctgg acaatcgaaa gccaccgcct
     3001 gctcaccatg ccggttcgca cctccgcact gcggacactg ggcgcgcaag gcaatgtgtt
     3061 cgcaatcgaa tccttcctcg acgaaatcgc ggccgagcgc ggcgaggatc cgctgatatt
     3121 ccggttgcga catctgtcgg atgagcgtgc acgagacgtg atccgctcgg tggcgaagcg
     3181 tgcgaactgg aagccagcgg ccaaggccgg cacaggttac ggtgtcggct ttgcgcgcta
     3241 caagaacacc ggcgcctatt gcgccgcgat tgccgagata gagggcgacg aggacattcg
     3301 cgtcagaaag ctgacgcttg cagttgatgt cggcgaagcg atcaatccgg acggcgtgag
     3361 aaaccagatc gaaggcggtg ccatccaggc gacgagctgg gtactcaagg agcgcgtgcg
     3421 atttgattcc gagcgcatca ccagcaatag ctggaccgac tatccgatcc tgcgtttttc
     3481 cgaggtgccg cgggtcgacg tcgaactgat ccagcgatcc gagaccgatc cggtcggcgc
     3541 gggcgaagcg gcgcacgccc cggtcaccgc cgcgattgcg aacgcggtgt tcgattgtct
     3601 tggcgtgcgc gtgcgcgatc tgccgatcac gcgcgacagc ctgcttgcgg cgatggaacg
     3661 cggttgatga acttggacat cctcagcggc ggcgccgcac aagggctggt cgcaagcctt
     3721 gcggccgcct tcaaggcaaa gaccggtttc gatattgcgg gcgaatatgg cgcagtcggc
     3781 agcatggcgg acaagctccg cgacggcgct cccgccgaca tgatcatcct gacgtcggcg
     3841 cttgttgcag aattggccga ggagaagctg gtcgtttcct cctcgatatc cgatgttggt
     3901 ttggttgaaa ctgcgctggc ggtgcgcagc ggcgatccaa aggtatcggc cacaactgtc
     3961 gacgatctgc gaaaagcgtt tcttgcggcc gacgcgatct tcgtgcccga caccaaagcc
     4021 tcgacggccg gaattcacgt ggccaaggtg ctcgaccggc tcggcatcgc cgatgaggtc
     4081 gccgctcgcc tgaaaatcca tccgaacggg gcgaccgcca tgcgggagct cgcagcgtcc
     4141 cgcgaaaggc gcccgatcgg ctgtacgcag tcgaccgaga tcatttccac gaaaggcgtt
     4201 gaattatcgg ggtcgctgcc caaggcttgc gcgcttgcga cgatgtacac cgccgccgta
     4261 gcggcaaagg ccgggcatgc cgcgcaggca aaagcgctga tcgagctgct ctcgagcgcc
     4321 gaaacgagcg aagcgcgccg ccgcgccggt tttctttagc cgctcagctc gaaagccctg
     4381 ccaggctggt cggcgatcgc aagtggagag tttcacggcg cggatggatg ccctccttgc
     4441 tccaattcaa cagttcatat actaatatat tagttcggga gagcgagcat gcgcgcgcgg
     4501 gtgacaaaaa aagccgaggc cgcatcgtcc tcgttgtcaa cagcgatccg ccgcaacggc
     4561 cggccgcgcg cggcgacggc gtcatcacgg atttacgccg atctgcgtgt cgagctcgtc
     4621 gcactgcggc ggcatccggg cgaggcgatt tcggaaggcg agatcgcgct gtcctatggc
     4681 gtaagccgga ctccggtccg cgaggcgatc ctgaaattgt cggacgaggg tctggtcgag
     4741 attttcccgc aatcgggcat catcgtgtcc cgcatcccgc ttgcggcgct gccggaagcc
     4801 atcatcattc gcaaggcatt ggaggaaacc acgacaaggc tcgcagccga actcgccacg
     4861 ccgagccagg tgctcgcgct gcagtcggtc ctgcaacggc agcgcgaagc cgacgcggcg
     4921 cgggatcgcg aggccttcca ccaggccgac gaattgtttc acgcaaccat cgccgagatc
     4981 gcgagatatc ctggaatctg gaagctgatc ctgcaggtga aggtcaatgt cgaccgcttc
     5041 cgccgcctca ccctgccgca ggcaggccgc atggcgcagg tgatagacga acatgagctg
     5101 atcctgtcgg caatcgaagc gcacgatgcg ccgcgcgccg cgctcgcgat gggcaagcat
     5161 ctcgaacggc tgctgcgtga cattgctgcc atccagaacg tcaatccgga gttcttcgac
     5221 gaacaacgtt agcaacgacg agaacgcgga cgagaagcga taaagcgagg gaggaatttg
     5281 acatgagacg ccgtgatttc ctcaagatcg gcgcggcgat gggcgccgct gctgcgagtt
     5341 tgccaccgct gtcctgcgca tctgcacagt ccaaagccac cttcaaggcc gccgacgtgc
     5401 agccggccgg ctacccaacg gtggcggcaa ccgaaagcct cggcaaaaaa ctcgaggctg
     5461 caaccggcgg gcgcctgtcg gtccagatgt atccgtcggc gcaacttggc ggcgagaagg
     5521 agaccatcga gcagacccag atcggcgcaa tccagctcct gcgcgtcagc gccggcgcgc
     5581 tgggcccgat cgtcgacgac gtcaacgtcg tcaacatgcc gttcctgttc aagaacacgg
     5641 cgcatgccga aaagatgatg gacggcccga tcggccagga cctgctcgac aggatctccg
     5701 ccaacgccaa tgccggcctc atcgcattgt gctggatgaa ctccggcgcg cgcagcatct
     5761 acaacaccaa aagggcggtc aaatcgatcg aggatctcaa gggattgaag atccgcgtga
     5821 tcggcaaccc gatcttcatc gacatgatga atgcgctcgg cggcaacggc gttgcgatgg
     5881 gttacgatca g
//
LOCUS       DATLJT010000384         1847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_22036,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000384 DATLJT010000000
VERSION     DATLJT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22036"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..516
                     /locus_tag="VM910_08835"
                     /pseudo
     CDS             304..516
                     /locus_tag="VM910_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
     gene            922..1395
                     /locus_tag="VM910_08840"
     CDS             922..1395
                     /locus_tag="VM910_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HVI62676.1"
                     /translation="MKWITRERVKVDRVACPWLIKKFVDRDAEFMFVPADKVVDEAKR
                     LNAILYDVKNVELGHHGKECSFEAILKKYKLSDDPALALLGKIVNGADTDNTLYQQPE
                     APGLEAIAEGFRHLGFKDDHAVNAAEWIVYDALYAYCREMVRQGKHNGDFVESAP"
     gene            1516..>1847
                     /locus_tag="VM910_08845"
     CDS             1516..>1847
                     /locus_tag="VM910_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62677.1"
                     /translation="MRLSHPSILLAASVVTFGAMQAVAGGLCKPALSLRDVRISEPRN
                     SQRTWSGVLVADASPCSTSSGPFEIEFTRLKETAPDLRFTERFTWASGRSEVSIDMWW
                     DEWFEDYR"
BASE COUNT          368 a          572 c          567 g          340 t
ORIGIN      
        1 gctggaaacg cgtgcgcgcg atgtgggagt gcgcacgctc aggctcgaga ccaatcggac
       61 cctcaaggaa gcgcaggcgc tgtatcgcag ttgcggatac gtggaagtcg agcccttcaa
      121 cgacgagccg tatgcgcatc actggtttga aaaaactcga atatgacgcg gtggatgcgc
      181 gaaccgtcgg gctggccgcc gcacaacgca gcgcagccgc cgcaggcgtt gtcatgcagc
      241 cgagataaat gcatgccacg ctattcggct gccgtcgaag cctcgcgctg aggggaagcg
      301 atcatgataa ccgttccgga gctcgcggcc gacgccctcg gcacttttct cgcgagctac
      361 atgcaacggc ggtatcgctc cgcagaaacc cgcctggtcg agctggtccc atcgatcgcc
      421 cgcattgcgc tggaatgcat cggcaacagc gatgcactgt accacaatgt cgagcacacg
      481 atgctggtca ccctcgcagg ccatgacatt ttacgccgcc attcgctatc tcaacgtcac
      541 ggcgaacggc cggcaatgga tcgccaatct ctactccaat gtttttcgcg ccgaacgcga
      601 cattgcgctc gggggaccgt aaaaatgatg ttgcgcggcc gcacccgacc gacggccaac
      661 ctctgctgat cgaactccac tttgcgccgc ccgtcatcgc cggcgcggtg caaagtgggt
      721 tttcggcact tctctccctc gcagccgccc cgcaaataag acctgagaaa aactaggccc
      781 gggtggagaa aagatgcttt gcccgcggcg cgattccgac ctctcctcca tcgagcacgc
      841 gcggcaatgg gcggtttcgc atcggcctaa gtactgatcg gccgatggcg gcgttcgcat
      901 gcaaggcaaa tggaggatcg catgaaatgg atcacgcgcg agcgcgtcaa ggtcgaccgg
      961 gtcgcctgcc catggctcat taagaaattc gtcgaccggg acgcggagtt catgttcgtc
     1021 cctgccgaca aggtggtcga cgaggctaag cgcctgaacg ccatcctcta cgacgtgaaa
     1081 aacgtcgagt tggggcatca cggcaaggag tgctcgttcg aggcgatctt gaaaaagtac
     1141 aagctgagcg acgaccctgc gctggcgttg ctgggcaaga tcgtcaacgg cgccgacacc
     1201 gacaatacgc tctatcagca accggaagcg ccgggcctcg aagcgatcgc ggaaggtttc
     1261 cgtcatctgg gcttcaagga cgatcacgcg gtgaatgccg ctgagtggat cgtctacgac
     1321 gcgctttacg cttactgccg ggagatggtg cgccaaggca agcacaacgg cgatttcgtc
     1381 gagtccgctc catagatccg ccaacgcgat ggcgcgcctc ggccgccggt cattgccgat
     1441 caagacagcc gaagtgccgc aaccgactta atcccgctca ccggcgcggg cgtcggcgat
     1501 cgaaaggagc ccgggatgcg tctctctcat ccttctattt tgctcgccgc cagcgtcgtc
     1561 acgttcggcg cgatgcaggc cgtcgccggg gggctctgca agccggcgtt gtcattacgg
     1621 gatgttcgca tttccgagcc gcggaactcg caaaggacgt ggagcggcgt gctcgttgcc
     1681 gatgcgtcgc cgtgctcgac cagctccggc ccattcgaga tcgaattcac ccgcctcaag
     1741 gagaccgcgc ctgatctgcg tttcaccgag cggttcacgt gggcttcagg gcggtccgag
     1801 gtgtcgatcg acatgtggtg ggacgagtgg tttgaggact atcgcat
//
LOCUS       DATLJT010000385         4446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_252031,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000385 DATLJT010000000
VERSION     DATLJT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252031"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VM910_08850"
     CDS             <1..330
                     /locus_tag="VM910_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HVI62678.1"
                     /translation="SGAFGPTTAGRMDSFVDQFQAAGGSMVMLAKGNRAPAVREACKK
                     HGGFYLGSIGGAAANLAEHCIKKVEVVEYPELGMEAIWRIEVVDFPAFIIIDDKGNDF
                     FKELNLG"
     gene            327..1001
                     /locus_tag="VM910_08855"
     CDS             327..1001
                     /locus_tag="VM910_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HVI62679.1"
                     /translation="MRALGLDGFTKGWVAVLLEGDRHQVTFHPNVAAALSLPFDRAAI
                     DIPIGMTDSGERCCDLLAREKLRPHASRVFTGARRWLWQEFSDPDRANSTARRRGQKS
                     VSRQLWHLGPKIMEVDAFVRANRSLDVREVHPELVFLRLNGGKPLLSKKTDEGLLLRR
                     RLLEHSGFRNIDRWLAEARIGTGARQDDVLDACAVAIAARDASTSLPQGTPPLDTCGL
                     PMQIWF"
     gene            1047..1493
                     /locus_tag="VM910_08860"
     CDS             1047..1493
                     /locus_tag="VM910_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVI62680.1"
                     /translation="MTVEWFDDLKVGMRFKSEKLTVSKEDILRFAAEFDPQPFHLDEA
                     AAEQTILNGLAASGWHTAAIAMRLAITARPFGPHPLFGAGVDELRWLKPVRPGDTLHL
                     EGEVVELHPSRTKPQGIARVKWTAYNQNGEAVYTFNPIAIVPTRPA"
     gene            complement(1519..1815)
                     /locus_tag="VM910_08865"
     CDS             complement(1519..1815)
                     /locus_tag="VM910_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62681.1"
                     /translation="MAVDGNWNLVMTTPMGERNATLSLKSAGSTLTGTQGADGNSTEI
                     FDGTVEGDDVAWKVSITNPMPLTLAFTGKVTGNSIAGEMGIGPMGNFPFTGTRA"
     gene            complement(1938..2501)
                     /locus_tag="VM910_08870"
     CDS             complement(1938..2501)
                     /locus_tag="VM910_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HVI62682.1"
                     /translation="MRDVFLRLGTDFLSTIIFLVTYVATDNIVLATSVAIAGAIGQVI
                     YARIRGRPLHFMTWASLALVIVLGGATILTRDPRFVLAKPAIAHFAIGAIMLKRGWML
                     RYMPPIAVETIPDMITFAGYAWAALMFALGGGTIAVAMTGDLKLWALYVSVVAVGAKV
                     LAFALQYVAFRILVTSRVRATRATAGP"
     gene            complement(2525..3109)
                     /locus_tag="VM910_08875"
     CDS             complement(2525..3109)
                     /locus_tag="VM910_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62683.1"
                     /translation="MTLTLFLILAPYGAFASLMLVVSATVSLAAAAVICLATITIDVV
                     RGRTIKLLPAASAVVFAGTAVYLNVFDPSLGSSKVRLAVDAGIFAISIGSMLIRYPFT
                     LQYALESVPAETAAMPGFLRANYIITAAWTVAALLMMLSNMAVLYIPGLPIWLSLLVA
                     VAARNSAVLFTKWYPQYRRTKYGAPPHDALPNPH"
     gene            3220..3951
                     /locus_tag="VM910_08880"
     CDS             3220..3951
                     /locus_tag="VM910_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI62684.1"
                     /translation="MGKKVKKTRARPGGSAKRSVPDRSATARQGSRAATSDHEAPYHH
                     GALRDALLEAAERVLEREGLAGLTLRAVAREAGVSHAAPTHHFGDLSGLVSDLAATGF
                     RQFNAAMAAAGAPDMPYPGRALARAKAYVAYAQAHPGMYGLMFRTERLDYSRPSLCEA
                     AEASFAALASAVAASRHEHIEQEALTLEQAAAIARVWSTVHGFTMLLLDGRLADVLKR
                     LPEGTDVEALLEAMLKLTMGKPVGT"
     gene            4186..>4446
                     /locus_tag="VM910_08885"
     CDS             4186..>4446
                     /locus_tag="VM910_08885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HVI62685.1"
                     /translation="MSAISPNKPYRVGRSRTGLGLFATKPIRKGAKIIRYFGPLLDCR
                     KKKDDAIENKYLFELNNRWTIDGSVRENIARYINHACKPNAES"
BASE COUNT          892 a         1334 c         1413 g          807 t
ORIGIN      
        1 tccggcgcgt tcggcccgac cacggcgggg cggatggatt ccttcgtcga ccagttccag
       61 gcggccggcg gctcaatggt gatgctcgcc aaaggcaacc gggcgcccgc ggtgcgcgag
      121 gcctgcaaga agcacggcgg cttctatctc ggctcgatcg gcggtgctgc ggcaaatctc
      181 gcggagcact gcatcaaaaa ggtcgaggtc gtcgaatatc ccgagctcgg catggaagcg
      241 atctggcgca tcgaggtcgt ggatttcccg gccttcatca tcatcgacga caagggcaac
      301 gacttcttca aggagttgaa cctggggtga gggcgctagg cctcgacggg ttcacaaagg
      361 gctgggtcgc cgttttgctg gaaggcgacc gtcaccaggt caccttccat cccaacgtcg
      421 cagccgcgct gtccttacca ttcgaccgcg ccgcgatcga catcccgatc ggcatgaccg
      481 atagcggcga acgctgctgc gacctgctgg cgcgcgaaaa attgcggccg catgcctcgc
      541 gcgtgttcac gggcgcacgt cgatggctgt ggcaggaatt cagcgatccg gaccgcgcca
      601 acagtacagc acgacgtcgt ggccagaaga gcgtctcgcg ccagctctgg catcttgggc
      661 cgaagatcat ggaagtggat gccttcgtgc gcgcaaatcg ctcgctcgat gtacgcgagg
      721 tccatcccga actcgtgttt ctgcggttga atggaggaaa gcccctgctg tcgaaaaaga
      781 ccgacgaggg actcttgctt cgccgcaggc ttcttgagca cagcgggttt cgcaatatcg
      841 accggtggct ggccgaggcg cggatcggga ccggagccag gcaggacgac gtcctggatg
      901 cctgcgccgt cgcgattgcc gctcgcgatg cgtcgaccag cctgccgcag ggcacgccac
      961 cactcgacac gtgcggattg cccatgcaaa tctggttttg atccagtcct gggatcgaat
     1021 gtggttttga tgcgagagtt gagaaaatga cggtcgagtg gttcgacgac ttgaaggtcg
     1081 gaatgcgctt caagagcgag aagctgacgg tctccaagga agacatcctg cgctttgcgg
     1141 ccgaattcga tccgcaaccg tttcatctcg atgaagcggc ggcggagcaa acgatcctca
     1201 acgggcttgc ggcgtcggga tggcacactg cagcgattgc gatgcggctc gccatcacgg
     1261 cgcggccgtt cgggccgcac ccgctttttg gggcgggggt cgatgagctg cgctggttga
     1321 agccggtcag gcctggcgac accctgcatc tggagggcga ggtggtcgaa ctgcacccat
     1381 cgcggacgaa gccgcaaggc atcgcccgcg tcaaatggac cgcctacaat caaaacggcg
     1441 aggccgtcta cacgttcaac ccgatcgcga tcgtgccaac ccggcctgcc tgaagattgg
     1501 ctcagccgcc gagcggcttc acgcccgcgt cccggtgaag gggaagtttc ccatcgggcc
     1561 aatgcccatt tcgcccgcaa tgctgttgcc ggtgactttt ccggtgaagg cgagcgttag
     1621 cggcatcgga ttggtgatcg agaccttcca ggcgacgtca tcgccctcta cggtgccgtc
     1681 aaaaatctcg gtggaattcc cgtccgcgcc ttgcgttccc gtcagcgtgc tgccggcgct
     1741 tttcaggctc agcgtcgcgt tgcgctcgcc catcggggtc gtcatgacga gattccagtt
     1801 tccgtccacc gccattgatg tctccttccg tgaccccgca caaaaccgga atggctttga
     1861 attttcgcaa aggctcgtcc ggcgatatag cgtatctcga aggccaagct caattcattc
     1921 gtccgcgtga aaccgtctca ggggccggcc gtcgcacggg tcgcgcggac gcggcttgtg
     1981 acgagaatgc gaaacgcgac atattgcagg gcgaaagcca gcacctttgc gccaaccgca
     2041 acgacggaca catagagcgc ccacaacttg agatcgccgg tcatggcgac agcgatggta
     2101 cccccgccaa gcgcgaacat cagcgccgcc caggcatagc ctgcgaaggt aatcatgtcc
     2161 ggaatagtct cgaccgcgat cgggggcatg tagcgcagca tccagccgcg cttcagcatg
     2221 atcgcgccga tggcaaaatg cgcgattgca ggttttgcca aaacgaagcg gggatctctc
     2281 gtcaggattg tcgcaccgcc cagcacgatg accagtgcaa gactggccca ggtcataaaa
     2341 tgcagcggcc ggcccctgat gcgagcgtag atgacctggc cgatcgcgcc ggcgatcgcc
     2401 accgacgttg ccagcacgat gttgtcggtc gcaacatatg tgacgaggaa gatgatcgtg
     2461 gagaggaagt cggtgccgag cctcaggaat acgtctcgca tcgtctgcct ccgggatgct
     2521 gcgatcagtg cgggttcggc agcgcgtcgt gcggcggggc gccgtatttc gtccgtcgat
     2581 attgaggata ccacttggtg aaaaggaccg cactgttgcg cgcggcgacc gcgaccagaa
     2641 ggctcagcca gatcggcagg cccgggatat aaagtacggc catattgctc agcatcatca
     2701 acagcgcagc cacagtccag gcagcggtga tgatgtaatt ggcgcgcagg aagcccggca
     2761 tcgccgcggt ctcggcaggg accgattcca gcgcatattg cagggtaaag gggtagcgga
     2821 tcagcatcga gccgatcgag attgcgaaga tgccggcgtc gaccgccagc ctcaccttcg
     2881 agctgcccag cgagggatcg aagacgttga gataaacggc ggtgccggca aacaccactg
     2941 ccgaggcggc ggggagcagt tttatcgtgc ggccgcgcac gacatcgatg gtgatggtgg
     3001 caaggcaaat taccgcagcg gcggcgaggc tcaccgttgc cgagactacc agcatcagcg
     3061 aggcgaaggc gccatagggc gcaaggatca ggaaaagggt tagggtcatg ccatagcctc
     3121 ttcgggccaa tctttacatt gtaaagatga atagcgacgg ggcgggcgcc ggtcaagtca
     3181 aatctttaca gtgtcaaaat tgcgccgtat ggaatgttta tgggcaaaaa ggtcaaaaaa
     3241 acccgcgctc gtccaggcgg cagcgcgaaa cgttccgtgc cggaccgatc tgccacggcg
     3301 cggcaggggt cgcgtgcggc tacatcggac cacgaggcgc cctaccatca cggcgcgctt
     3361 cgagacgcgc tactggaagc ggccgagcgg gtactggagc gcgaggggct tgccgggctg
     3421 acgctgcgcg cggttgcgcg cgaagcgggc gtgtcccatg ccgcgccgac ccatcatttc
     3481 ggcgacctct cggggcttgt cagcgatctt gctgcgaccg gcttccggca attcaacgct
     3541 gcgatggcgg cggccggcgc gcccgatatg ccctatcccg ggcgggcact ggcccgcgcg
     3601 aaagcctatg tcgcttatgc ccaggcccat cccggcatgt acgggctgat gttccgcacc
     3661 gagcgcctgg attattcgcg gccgtcgctg tgcgaggccg ccgaagcctc tttcgcggca
     3721 ttagcgagcg cagttgccgc cagccgtcac gagcatatcg aacaggaagc gttgacgctg
     3781 gagcaggcgg cagcgattgc gcgcgtctgg tcgacggtgc acggcttcac tatgctgctt
     3841 ttggacggac ggctggcgga cgtcttgaag cgcctgcccg aggggacgga cgtcgaggcg
     3901 ctgctcgagg ccatgctcaa gctgaccatg ggcaagccgg tcggcacgtg aggtcagcgc
     3961 cgcgtcgcga gctgcgcgct tcgatgtgac aaatttgcaa aaggatgcaa gagagcgcgt
     4021 cacacaagcg atatagcatc cttgtcatcg aattttttag tgtgaagtgt acaatttggg
     4081 gaaggttcac aagaagagcg accaccacct gacatttccg cggaattgtt ctagaagagc
     4141 ggcttcgctt tccattctcg ttgaccgcgc gtcagttcgt tacacatgtc tgcaatttcg
     4201 ccgaacaaac cctatcgcgt cggccgttcc cgtaccgggc taggcctctt cgccaccaaa
     4261 cccatcagga agggtgccaa gatcatccgc tatttcgggc cgttactcga ttgccgaaag
     4321 aagaaggatg acgcgatcga gaacaaatat ctgttcgaac tcaacaaccg ctggaccatc
     4381 gacggttcgg tgcgcgagaa catcgcccgc tacatcaatc acgcctgcaa gccgaacgcc
     4441 gaaagc
//
LOCUS       DATLJT010000386         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_252043,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000386 DATLJT010000000
VERSION     DATLJT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252043"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VM910_08890"
     CDS             join(<1..315,317..913)
                     /locus_tag="VM910_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936016.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI62686.1"
                     /translation="NDLRKRAIDEVGSGASRREAAEQFGVSPSAVIKWVRRWCDTGSA
                     TAKPSGGSVSPLEKYAQWFFDLIAAQPDLTLDEVMVAKKAARIPGSRSAVGRFFRRHD
                     LTVKKSLYAAEQQRADVARARRRWIREQGLLDPARLVFIDETATSTNMVRLRGRCPRG
                     VRLVGYAPHGHWKTITFVAGLRHDRMVAPFVMEGAMNGLTFVEWVRQFLVPTLKRRDV
                     VIMDNLPSHKVPGVREAIESAGATLRYLPQYSPDLNPIENCFSKIKAHLRKAAERTIN
                     RLCRRIGKIASSVSPDHALSGAFSHAC"
     gene            complement(1028..2404)
                     /locus_tag="VM910_08895"
     CDS             complement(1028..2404)
                     /locus_tag="VM910_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HVI62687.1"
                     /translation="MIDDTLLPFDLPAVRRKKLTVDFGGGTQSSDAGLLLLREAERKL
                     GVCRRLADAMPDRRDPDRIQHVMFEMVMARASAIACGHKDAIDLDRLRHDPLMKVAVG
                     RCPETGAALASQSTISRLENAPSKTEAARLAVALLDQLGTTVKAGKLEILDIDDTFCA
                     AHGGQQLAFWNAHHDERGFASMHIYHAASGTPVATILRPARTPKGTEVRTVIKHVTKR
                     LRQHWPNTHIVWRGDSHYGRVEAMEWAEADGAGYIFGLAGNAPLDALVAETADNLRFH
                     HAKSSQMKLRTYVSIMYQAGSWSRPRKVVARLECSLQPGAGEGTSTGMRQEVDIRYVV
                     TSLKGSAQHLYENVYCQRGQMKNLIKLHKAQLASDRMSCHSATANQVRLVLHTAAFWL
                     MHGVRATIPQTSPLATAEFATIRERLIKIGARVIEHIARIRIQLPTSCPEGALFRAVA
                     LGLMPSGP"
BASE COUNT          482 a          832 c          837 g          495 t
ORIGIN      
        1 aatgaccttc gcaagcgcgc tattgacgag gttgggtccg gcgcttcgcg gcgcgaggcg
       61 gccgagcaat ttggcgtgag cccgagcgcg gtcattaaat gggtgcggcg ttggtgcgat
      121 acgggaagtg cgacggccaa gccgagcggc ggcagcgtat cgccactgga aaaatatgcg
      181 caatggtttt tcgacctgat tgctgcacaa cccgatttga cgctggatga ggtcatggtg
      241 gcgaaaaaag cggcgcgcat ccccggcagt cgcagtgccg tggggcgttt cttccgtcgc
      301 cacgatctca ccgtcaaaaa aaagcctgta cgccgccgag cagcagcgag cggacgtggc
      361 gcgtgcacgc cggcgttgga tacgcgagca aggtttgctt gatccggcgc ggctggtctt
      421 tatcgacgag acggccacca gcaccaacat ggtgcggttg agaggccgct gcccgcgtgg
      481 cgtgcggctg gtcggctatg cgccgcatgg acattggaag acgatcacgt tcgtcgccgg
      541 actacgccac gaccgcatgg tggcgccttt tgtgatggaa ggagcgatga acggtctcac
      601 gtttgtcgaa tgggtgaggc agtttcttgt cccaacgctc aagcgtcgcg atgttgtcat
      661 tatggataat ctgccttcgc acaaagtgcc gggggtgcgc gaggcgattg aatctgccgg
      721 tgcaacgctg cgttatctgc ctcaatattc tcccgatctc aacccgatcg agaactgctt
      781 cagtaaaatc aaagcgcatc tgcgcaaggc cgcagagcga accataaacc ggctttgccg
      841 ccgcatcggc aagatcgcaa gctcagttag cccggatcat gcactaagcg gggcattttc
      901 gcacgcttgt tgaccgcgcg cgcgttgacg atccgaccgg ctggatcgtg tgagcaactc
      961 gctgtgcgac gcgtttgggg ttgatctgcc atggtcggcg gcacgttggg cgctcggccc
     1021 ccgcggctca cgggccggac ggcatgaggc cgagtgcgac ggctcggaac aacgcaccct
     1081 ctgggcagct cgtcggcagc tggatgcgga tgcgcgcgat gtgctcgatg acgcgcgcgc
     1141 cgatcttgat gaggcgctcc cggatcgttg cgaactcggc cgtcgccagc gggctcgtct
     1201 gcgggatcgt ggcgcgcacg ccgtgcatca gccaaaatgc agcggtgtgg aggacgagcc
     1261 gtacctgatt ggccgtcgcg ctgtgacacg acatgcggtc cgatgccagc tgcgccttgt
     1321 gcaacttgat caggtttttc atctgcccgc gctggcagta gacgttttcg taaagatgct
     1381 gcgccgaacc cttgagcgag gtgacgacgt agcggatgtc gacctcctgg cgcatgccgg
     1441 ttgaggtgcc ctctccggcg cccggttgca gcgagcattc gagtcgcgcc accactttgc
     1501 gcggccggct ccaactgccg gcctggtaca tgatgctcac ataggtgcgc agcttcatct
     1561 ggctgctctt ggcatggtgg aaacgcagat tgtcagcggt ctcggcgacc agcgcatcga
     1621 gcggcgcatt gccggcgagg ccgaaaatat aaccggcgcc gtccgcttct gcccactcca
     1681 tcgcctcgac acgaccgtaa tgactgtcgc cacgccacac gatgtgggtg ttcggccaat
     1741 gctgcctcag gcgcttcgtc acgtgcttga tgacggtcct gacctccgtt cccttcgggg
     1801 tacgggccgg ccgcaagatc gtcgcgaccg gcgtgccgct cgccgcgtga tagatgtgca
     1861 tcgaggcaaa gccgcgctcg tcgtgatgcg cgttccagaa tgcaagctgc tggccgccat
     1921 gcgcggcaca gaacgtgtcg tcgatgtcga gaatctcaag cttgcccgcc ttgacggtcg
     1981 tgccgagctg atcgagcaga gctaccgcaa gccgcgccgc ctcagtcttg ctcggcgcat
     2041 tctccaggcg actgatcgtc gattgcgacg ccagcgctgc ccccgtctcc ggacagcgac
     2101 cgaccgcgac cttcatcaag gggtcgtgcc gcagccggtc gaggtcgatc gcatccttat
     2161 gcccgcaggc gatcgcagac gcgcgcgcca tcaccatctc aaacatcacg tgttggatgc
     2221 gatccggatc gcgacgatcg ggcatcgcgt ccgcaagccg ccggcacaca ccaagcttgc
     2281 gctccgcctc ccgcagcagc agcagaccgg catcggacga ctgtgtgccg ccgccaaaat
     2341 cgactgtgag cttcttgcgg cggacagctg gcaggtcaaa gggaagcagc gtatcgtcga
     2401 tcatggcggc tgtggcagag agctgatacg cgaggcaact cgtccgaaca ccgatttgct
     2461 acttgaatcg ctcacttcac gccacagtcg ccgcaaaaat ctcatctccg tgcataatcc
     2521 gggctaacgc gttatgtccc gcgcacgcgt tagcaaggtg ccgcgggacg accgaacgcc
     2581 gcccgcaaaa caccgccctt gttcgtcaca accgcagatg ggcaccggcg caccgcaacc
     2641 ccgccc
//
LOCUS       DATLJT010000387         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1630289,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000387 DATLJT010000000
VERSION     DATLJT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630289"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1479
                     /locus_tag="VM910_08900"
     CDS             223..1479
                     /locus_tag="VM910_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI62688.1"
                     /translation="MPRRSKGARLWPESEERDARGKLVRHATWVIRDGTRKVRTGCAR
                     EDRAGAERALAEYLASKYRAPRDRNRHPADILVLDVLNIYLADKAGRHARPEETKQRV
                     LTLADFWQPHTLADVNGKRCRDYVAWRVGQPWRSAKPDQTGNRPRLVTEAAARRELED
                     LRAAINHHRQEGLCSEIVSVVLPEKGVARERWLTRSEAARLIWAAWRTRQVMRDQRTK
                     RAVGRHVARFILLGLYTGTRHAAICSAALMPTIGRGHVDLEQGLFYRRAEGAHETKKR
                     RPPVRLPKRLLTHLRRWERLGIAKRAVVEWNGKPIRSVRKGFAAAVRAAGLDGTVTPH
                     VLRHTAATWAMQNGADLWQAAGFLGMTVELLQGRYGHHHPDFQREAAEAVAGSPGQKW
                     DRNTVNKTRRTSSKVTKITEISKGAR"
     gene            complement(1755..2357)
                     /locus_tag="VM910_08905"
     CDS             complement(1755..2357)
                     /locus_tag="VM910_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI62689.1"
                     /translation="MTVAYSQTGWAAASPARAAATEAFSDEVLIRRIANGDQLAMRTL
                     FSRHRLAIYRWLLRLVDDEALADDLLSDVFLDVWRKATSFERRSSVSTWLLAIARHKA
                     LSARRRRSDVELNAELASTVADPADNPELVLQKKNREELLRHSLARLSPEHGEVIDLT
                     YYHGKSIKEIAEIVGINEATVKSRTFYARKRLAQLVASAM"
     gene            2767..>3411
                     /locus_tag="VM910_08910"
     CDS             2767..>3411
                     /locus_tag="VM910_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62690.1"
                     /translation="MIPSVNFARGFAACLAALTWGGIGTASALDYPTRPVRWIVTYTP
                     GGGTDITARIMAQWLSERLGQQFIIENKPGAGNNIGTEAAIHSPPDGYTLLLVNPANA
                     INATLHPKLSFNFIRDIAPVAGIMRVPNVMEVNPSVPAKTVAEFIAYAKANPGKVNWA
                     TSGNGTSVHLSGELFKMMTGVEMTNIPYKGSAPALTDLIAGTVQVIFDNMPPSLP"
BASE COUNT          685 a         1050 c         1100 g          576 t
ORIGIN      
        1 tgccaaggaa tgccgacaaa tggcgagcaa gtatcccccg cgtgaggata ttggccatga
       61 tcagccgctt cggctggccg tggctgctgc gctcgccttc cccgacggct cgatgactgc
      121 cagtggatta cgccgtgaag cagcccgcgg acggcttgtg gtcgagcgta tcgccggcaa
      181 ggattacacc accttggcca atatcgaacg aatgagggag ctatgccgcg tcgaagcaaa
      241 ggagctcgcc tgtggcccga atcagaggag cgcgacgcgc gcggcaaact cgtccgtcac
      301 gcgacctggg tcatccgcga cggaacgcgt aaggtccgca cgggctgcgc tagagaggac
      361 cgcgcggggg ctgagcgggc gcttgccgag tacctcgcca gcaaatatcg agccccacgc
      421 gatcggaacc gtcatccagc tgacatcctc gtcctcgacg tcctgaacat ctatctcgcc
      481 gacaaggcgg ggaggcacgc caggccagag gaaacgaagc agcgtgtctt gacgctcgcc
      541 gacttctggc agccccacac gcttgcggat gtaaacggca agcgctgccg cgattacgtt
      601 gcatggcgcg ttggccagcc gtggagatcg gccaagcccg accagacggg aaatcggccg
      661 cggctagtga ccgaggcagc cgcacgccgg gaactggagg accttcgcgc cgccatcaac
      721 catcaccgcc aggaaggctt gtgctcggag attgtgtcgg tggtacttcc cgagaagggg
      781 gtcgcacgag agcgctggct cacgcgatcg gaggcggcgc ggctgatctg ggcggcgtgg
      841 cggacgcggc aggttatgcg ggatcagcgc accaagcgcg cggttggccg gcatgtggcc
      901 cgcttcatcc tgctcgggct gtacaccggc acccgccatg cggcgatttg cagtgctgca
      961 ctgatgccga ccatcggacg cgggcatgtc gatctcgagc agggcctgtt ctatcggcgt
     1021 gcagaaggcg cgcatgagac caagaagcgg cggccgcccg ttcggctccc caagcggctg
     1081 ctgacgcact tgcggcgctg ggaaaggctc ggaatcgcca agagagcggt ggttgagtgg
     1141 aatggcaagc caatccgctc cgtgcgtaag ggatttgcgg ctgccgtcag agcggctggc
     1201 cttgatggca cggtcacgcc tcacgttctg cgccacactg ctgcgacctg ggcgatgcaa
     1261 aacggcgccg atctatggca ggcggcgggc ttccttggga tgacggttga actgctgcaa
     1321 ggccgctatg ggcatcacca tcccgacttc cagcgcgagg cggcggaggc agtcgctggc
     1381 tcaccgggac agaaatggga cagaaacacc gtgaacaaaa cgcgacgaac gtcgtcaaag
     1441 gtgacgaaaa ttaccgagat ttcaaagggg gcgcgataag acgcctcgtt tgggacgagg
     1501 gggtcgcagg ttcaaatcct gccactccga ccaaataccc tctggcgatg ctgaatttcc
     1561 acaactatcc gcagcgcctt atcaatttcg aagcgtcgtg cgcaaaggcg tgcgcagcgt
     1621 acacagacga gtgttgcgga tggttggacc ttgcggccgc actgaagcgg ctcattgagg
     1681 cgcgatgaca gccccgataa ccctatgagc gatcacgcga gcagaaatcc caatcgccgc
     1741 aaacgagtac gcgttcacat cgcagatgcg accaactgcg cgagcctctt gcgcgcgtag
     1801 aaggtgcgcg acttcaccgt ggcctcgttg atgccgacga tctcggcgat ctccttgatg
     1861 gatttgccat gatagtaggt gaggtcgatg acctcgccgt gctccggcga taaccgagcc
     1921 aaggaatggc gcagcagttc ttctcggttt ttcttctgta ggacaagctc cgggttgtcc
     1981 gcagggtctg cgaccgtgga cgcaagttcg gcgttgagtt caacgtcgct gcgacggcgg
     2041 cgagcggaga gcgccttgtg gcgcgcgatg gccagcagcc aagtcgacac cgacgagcgc
     2101 cgctcgaagg aggtggcttt gcgccagacg tcgaggaaga catcgctcaa gaggtcgtct
     2161 gcgagggctt cgtcgtccac caggcgcagc agccagcggt agatcgccaa gcgatgccgc
     2221 gaaaacagcg tccgcatggc aagctgatcg ccatttgcga tccgccggat taacacttca
     2281 tccgagaacg cttcggtcgc cgctgcccga gcaggcgagg cggcggccca tcccgtctgg
     2341 gagtaggcca cagtcattgg gcatcccctg taaacacgtc ccgcccaaca aaacgccccc
     2401 agcagaaaaa ggtttcggac ccgtggccga ccgtttggct gtgagtgtta cccccgagcg
     2461 gatacccact atggttaact cggaatggtt aagaacggcc taagcggaat ggttaagaac
     2521 tgcctaagcg gagcggtgaa ggtctcggca agctttgttg attgacgcat cccccggcga
     2581 tggagcgagg cgacgcgagt cgcgcgcgca aatcatgcgg tttctcgaac aaggggcgat
     2641 cggccaacgc ccttgtgcga gggcgccgat gccgcgtacc ttcgcccgca agttcgcgaa
     2701 cgggaccgcg acggccaaag cggcatcgtt cgccccaaca aaagccggca gcaggagacg
     2761 agcgccatga tcccaagcgt gaattttgcc cgtgggtttg ccgcgtgtct ggccgctctg
     2821 acgtggggcg ggatcggcac cgcctcagcg ctcgactacc cgacccggcc ggtgcgttgg
     2881 atcgtgacct atacccccgg cgggggcacg gacatcacgg cgcgcatcat ggcgcaatgg
     2941 ctgtccgaac ggctggggca gcagttcatc atcgagaaca agccgggcgc gggcaacaat
     3001 atcggcaccg aagcggcgat tcattcaccg cccgacggct acacgctgct gctggtcaat
     3061 ccggcgaatg ctatcaatgc aacgctccat cccaagctgt ccttcaactt catccgcgat
     3121 atcgcgccgg tcgccggcat catgcgggtg ccaaacgtga tggaagtcaa tccgtcggtg
     3181 ccggccaaga ccgttgccga gttcatcgcc tacgccaagg ccaatcccgg caaggtcaat
     3241 tgggcgacct cgggcaacgg cacctcggtc catctctcgg gcgagctgtt caagatgatg
     3301 actggcgtcg agatgaccaa tattccctac aagggctcgg cgccggcgct caccgacctg
     3361 atcgccggca cggtgcaggt gattttcgac aacatgccgc cgtcgctgcc g
//
LOCUS       DATLJT010000388         1991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_711065,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000388 DATLJT010000000
VERSION     DATLJT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711065"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VM910_08915"
     CDS             complement(<1..923)
                     /locus_tag="VM910_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62691.1"
                     /translation="MPRLDVLDEEFGEDAVLHARRRRTGERLFMLAIVALGLGAIGAL
                     AFAWSNADGPLRFELQLAAAAPKSAQRAAAQEEIGGAAVAWPVTAHAQRPKMLRVGYS
                     GILPRGAPHYAAFERRMAELGYQPGRNFTFEYIQAPSIEGYELTYRELAARKVDILLA
                     AGNEPALRAARSAAGATPTVFIALDFDPVEKGYVASLSRPGSNSTGLFVSQLELAGKR
                     VELLRKTFPKARRVGLLWDATSREQADAAAAVAGQLAFEPRLLEVTGQPPNYAAALMP
                     MGQWPGEPIMIPASPLALRDRTTIVRLLFDRR"
     gene            1269..1448
                     /locus_tag="VM910_08920"
     CDS             1269..1448
                     /locus_tag="VM910_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62692.1"
                     /translation="MEGQQSRRSSGRWYILGLICLMYLITYLDRVNISTAAPVISNEF
                     GFDKVTMGAIFSAFV"
     gene            1458..>1991
                     /locus_tag="VM910_08925"
     CDS             1458..>1991
                     /locus_tag="VM910_08925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62693.1"
                     /translation="MFQVPGGWLSDRFGARPVLAAIVAYWSAMTAATAAATGAISFIV
                     LRFLFGIGEAGAFPGATRSMQLWYPREERGLVQGLTHSASRLGAAIAPPLVVLIMINL
                     GWRSVFYICGAVGLVWSIWWYLSYRNLPEEHRRVNRAELEHIRGVDNEGNIKEPAIER
                     KAASVPWGTLLRSPNMWA"
BASE COUNT          344 a          619 c          629 g          399 t
ORIGIN      
        1 cggcggtcga acagtaggcg cacgatggtc gtccgatcgc gcagcgcgag cgggcttgca
       61 gggatcatga tcggctcgcc gggccattga cccatcggca tcagggcagc cgcatagttc
      121 ggcggctgcc cggtcacttc cagcaaccgc ggctcgaagg ccagttgtcc ggccaccgcg
      181 gcggcggcat cggcttgctc ccgcgatgtc gcatcccaaa gaagtccgac gcgacgtgcc
      241 ttcgggaaag tcttgcgcaa gagctcaacg cgttttcccg ccaactcgag ctggctaacg
      301 aagagcccgg ttgaattgct gcccgggcgt gacaggctcg ccacatagcc cttctcaacc
      361 ggatcgaagt cgagcgcgat gaagacagtt ggcgttgcgc ctgcggccga ccgcgccgcc
      421 cgcagcgccg gctcgttgcc ggccgcaagc aggatatcca ctttgcgcgc ggcaagctcg
      481 cgataggtca attcgtaccc ctcgatgctt ggcgcctgga tgtactcgaa cgtgaaattg
      541 cggcccggct ggtaaccgag ttcggccatg cgcctctcga aggcagcata atgcggcgcc
      601 cctcgcggca ggatgccgga gtagccgacg cggagcatct tgggccgctg cgcgtgggcg
      661 gtgaccggcc acgcgaccgc cgcgccgccg atctcctcct gcgctgccgc gcgctgcgcg
      721 cttttcggcg cggcagccgc caattgcagc tcgaaccgca gcggcccatc cgcgtttgac
      781 caagcaaagg cgagcgcacc gatggcgccc aggcccagcg ccacaatggc cagcatgaaa
      841 agccgttcgc ctgtccggcg gcggcgcgca tgcagaaccg cgtcctctcc gaactcctcg
      901 tcgagcacgt cgagcctggg catgtccggc ggttccaatc gactcggcat caccgctttc
      961 actgtcggct ggcgttgtac tggtcgcgcg ctgcgctgcg aacccctgca cgcctatttt
     1021 cccgtccggc gcggcggcgc gccacatgat gcgctcatag caccagcatc tcaagctgcc
     1081 cggtagcgtg gaaaatctgg ttcccaacga tgtttttctt ttatggatga agagatcgca
     1141 cgtcgtcatc tctaccaagt ggagaggcac tcgcggacgc tcgcgcgcgg ctgagccgcc
     1201 agcgcgcatt gataggacga cacgccatcg gattgacatt gagagaagcg atccagggag
     1261 gattgagaat ggaaggtcaa caatcgcgcc ggagcagcgg acgctggtac attcttggtt
     1321 taatttgcct gatgtacctc atcacctacc tggaccgggt caatatctcg acggctgccc
     1381 cagttattag caatgagttt ggctttgaca aggtcacgat gggggcgatc ttcagcgcct
     1441 ttgtctaggc ctatgccatc tttcaggtgc ccggcggctg gctcagcgat cgctttggcg
     1501 cacggccggt gttggcggcc atcgttgctt actggtcagc gatgaccgca gccactgcgg
     1561 cggcaactgg cgcgatttcg ttcatcgtgt tgcggttttt gttcggaatt ggagaggctg
     1621 gcgcctttcc gggggccacg cgctcgatgc aactttggta tccacgcgag gagcgcggtt
     1681 tagtccaagg actcacgcac agtgcgagtc gtcttggtgc cgccattgct cccccgctcg
     1741 tcgtcttgat catgatcaat ttgggctggc gatcggtctt ctacatctgc ggtgcggtcg
     1801 gtttggtctg gtcgatctgg tggtatctta gttatcgcaa tttgccagaa gagcatcgtc
     1861 gcgtaaaccg cgcggagctg gagcacattc gtggcgtgga caacgagggc aatatcaagg
     1921 agccagctat cgaacgtaag gcggcgtccg tgccttgggg cacactgctg cgttctccga
     1981 atatgtgggc g
//
LOCUS       DATLJT010000389         3130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2549927,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000389 DATLJT010000000
VERSION     DATLJT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549927"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM910_08930"
     CDS             <1..318
                     /locus_tag="VM910_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HVI62694.1"
                     /translation="AQVTSSEVIKFSAGGFRDFTRIAASDPTMWRDVFLSNKEAVLEM
                     LGTFNEDLSKLTRAIRRGDGEALFEHFTRTRAIRRGIVETGQDSAAPDFGRPHEHLAK
                     RPD"
     gene            complement(363..614)
                     /locus_tag="VM910_08935"
     CDS             complement(363..614)
                     /locus_tag="VM910_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62695.1"
                     /translation="MTKYRIIGIAVVLSAAIATPALAGVIDEPGLYAFYHPNGDLGFG
                     PSRPSDAMASQTMRGDSVAGMRMSVRAHHIARAPAIKRH"
     gene            complement(809..2032)
                     /locus_tag="VM910_08940"
     CDS             complement(809..2032)
                     /locus_tag="VM910_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HVI62696.1"
                     /translation="MTSASGNSGESGEDITPAPRRRPLWQLFIAPGLLLIAAIAWSVF
                     WFYASSQVDVQADAWKAREAKSGRIYDCANRSVGGYPFRLEIRCDDPSVALVSQAANQ
                     AATPITAKLGEILVVAQVYDPKRVIAEFKGPATISDPADQSTFVANWSLGRSSVVGLP
                     ATPQRVSVVFDQPAIDRVQGSVQVPLAGARHIELHGRLAEGSTTTSPVIESVVQISGG
                     SIRGLHPLLAEPFDADVRAMLSGLKDLAPKPWPARFRDIQAAGGHVEIVQSRIQQGDL
                     IAVAAGTLGFSPSGRIEGELQMTVAGIEKVIPALGIDKMLEEGVPQATLDRVAPGVKT
                     QDITNLMGALDRAVPGLGKIVKQNANVGVAAGINALGKETVLEGKKARSFPLRFVDGA
                     VFLGPLKVAQTPPLF"
     gene            2306..2902
                     /locus_tag="VM910_08945"
     CDS             2306..2902
                     /locus_tag="VM910_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HVI62697.1"
                     /translation="MSTVTLPEPESSKGDLWVFGYGSLIWRPGFEFIEQVPARLIGEH
                     RALCVYSFDHRGTPEKPGLVLGLDRGGACRGVAFRVAAKRRESTIDYLRSREQTTHVY
                     REVMRSVWLDNEARGRVSALTYVVDRGHVQYAGRLPISEQFRIVRQGHGRSGNNRDYV
                     LSTVRAIEARGFRDTQLHQLALMLHDDPHAHHVPVEGP"
     gene            complement(2912..>3130)
                     /locus_tag="VM910_08950"
     CDS             complement(2912..>3130)
                     /locus_tag="VM910_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HVI62698.1"
                     /translation="LNSGLFWPRRTFMRYPGTLIVEFLDPLPPGLPREEFLTRLQSTI
                     EEATGRLVAAGEAEQARLFGGVPRSAKA"
BASE COUNT          570 a         1026 c          963 g          571 t
ORIGIN      
        1 gcgcaggtca cctcctcgga ggtgatcaaa ttctccgccg gcggctttcg cgatttcacc
       61 cgcatcgccg cctccgatcc aaccatgtgg cgcgacgtgt tcctgtccaa caaggaggcg
      121 gtgctggaga tgctcggcac cttcaacgag gatctctcca agctgacgcg ggcgatccgc
      181 cgcggtgacg gcgaggcgtt gttcgagcat ttcacccgca cccgcgccat ccgccgcggc
      241 attgtcgaga ccgggcagga ttcggccgcg cccgatttcg gccgacccca tgagcatctc
      301 gcgaagcggc cggattgagg gccggccgct cttcctcgtc aatcgcttcg agcaattttc
      361 gttcagtgac gcttgatggc gggtgcgcgg gcgatgtgat gtgctctcac cgacatccgc
      421 attcccgcga cgctgtcgcc gcgcatcgtc tgcgaggcca tcgcatccga aggccgcgaa
      481 ggtccgaagc cgaggtcgcc gttcggatga taaaaggcgt agaggcccgg ctcgtcaatc
      541 acccctgcaa gcgccggcgt tgcgatcgcc gccgacagaa caaccgcgat gccgataatc
      601 cggtacttcg tcatggacgt tctccttgtg atcgatccgc accgatgtgc gtgccgtctg
      661 cggaacagga ctgtgaatta ggtcccgaca ttcccgccgc acaggagaag ccgaatcacg
      721 cttcggcgat cacagcgtga gcttgcgcat gcaaccattc gcctgatcgt gacgatttga
      781 gaattccgaa atcaggcctt gggacgtttc aaaacagcgg cggcgtttgc gcgaccttca
      841 gcggtccgag aaagaccgca ccgtcgacga agcgcaacgg aaagctgcgc gccttcttgc
      901 cttccagcac ggtttccttg ccgagcgcat tgatgccggc ggcaacaccg acattggcgt
      961 tctgcttgac gatcttgccg agcccgggga ccgcgcggtc caacgcgccc atcaggttcg
     1021 tgatgtcctg cgtcttgacc ccgggcgcga cgcgatcgag cgttgcctgc ggcacgccct
     1081 cctccagcat cttgtcgatg ccgagcgctg gaatcacctt ctcgatgccc gctaccgtca
     1141 tctgcaactc gccctcgatg cggccgctcg ggctgaagcc gagcgtgccg gccgcaactg
     1201 cgatcagatc gccctgctga atgcgcgact gcacgatctc gacgtgcccg ccggcggcct
     1261 ggatgtcgcg aaaccgcgcc ggccagggtt tgggcgcaag gtccttcagc ccgctcagca
     1321 tcgcgcgcac gtcggcatcg aacggttcgg cgagcagcgg atgcaaaccg cggatgctgc
     1381 ctccggaaat ctgcaccacg ctttcgatca ccggcgacgt cgtcgtcgac ccctccgcaa
     1441 gacgcccgtg cagttcgata tgcctcgcgc ccgccagcgg cacctgcacc gagccttgga
     1501 cgcgatcgat tgccggttga tcgaatacga cggaaacgcg ctgcggcgtc gctggcaatc
     1561 cgacgacact gctgcggccc aggctccaat ttgcaacgaa ggtcgactgg tcggcgggat
     1621 cggaaatcgt cgccggtccc ttgaactcgg cgatcacccg ctttggatcg tagacctgcg
     1681 ccacaaccag gatctcgccc agcttcgccg tgatcggcgt cgcggcctgg tttgcggcct
     1741 gcgacaccaa cgcgacgctg gggtcatcgc aacggatttc gaggcggaac ggataacctc
     1801 ccaccgaacg gttggcgcaa tcgtaaatgc ggcccgattt cgcttcgcgc gccttccagg
     1861 cgtcggcttg gacatccact tgcgaagagg catagaacca gaatacgctc catgcgatgg
     1921 ctgcgatcag gagcagtccg ggggcgatga aaagctgcca cagcgggcgc cgccgcggcg
     1981 cgggcgtgat gtcctcaccc gattcgccgc tattgcctga tgcggacgtc aaatccaagc
     2041 tccctgccgg gcatcggcga ccctttgatc agcaattttg gcaataaagc ggtcgcgccg
     2101 cagctaatca agttaacgca ttttcatgga agatcgtttt ctgctagtgg agcgcctctt
     2161 gccgcgcttc ctcagctagc gaaggtcaaa tgcaaacctc cactggtaac aataacttgc
     2221 cggtggtccg gtgaccccga cttcgcaaaa ccggctgccc ggatgggaca cgaatgttag
     2281 gatcggatcg ctggcgattc acaagatgtc cactgtcacc ctccccgaac cggaatcttc
     2341 caaaggcgac ctctgggtgt tcggctatgg ctcgctgata tggcggccgg gcttcgagtt
     2401 catcgaacag gtcccggcgc ggctgatcgg cgagcaccgc gcgctctgcg tctattcctt
     2461 cgaccatcgc ggcacaccgg aaaaaccggg cctggtgctg gggctcgatc gcgggggcgc
     2521 ctgtcgcggc gtcgcattcc gtgtcgcagc gaagcggcgc gagagcacga tcgactacct
     2581 gcgcagccgc gagcagacga cccacgttta tcgcgaggtg atgcgatcgg tctggctcga
     2641 caacgaggcg cgcgggcgcg tcagcgcgct gacctatgtg gtcgaccggg ggcatgttca
     2701 atatgccggc cgcctgccga tttcggagca gtttcgcatc gtccggcagg ggcacggccg
     2761 ttcaggcaac aaccgcgact atgtgctctc cacggtgcgg gcaatcgagg cccggggatt
     2821 tcgcgacacc caactgcatc agcttgcgct gatgctgcat gacgatccgc acgcccatca
     2881 tgtccccgtg gaaggaccgt aagaacgaca atcaggcttt ggccgagcgc ggcacaccgc
     2941 cgaacagtct cgcctgctcg gcttctcctg ccgcaacgag gcggccggtc gcctcctcga
     3001 tcgtactttg aaggcgcgtg agaaactcct cgcgcggcaa gcccggcggc aacggatcga
     3061 ggaattcgac gatgagggtg ccgggatagc gcatgaaggt gcggcgcggc cagaacagac
     3121 cggaattgag
//
LOCUS       DATLJT010000390         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3468894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000390 DATLJT010000000
VERSION     DATLJT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3468894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VM910_08955"
     CDS             complement(<1..937)
                     /locus_tag="VM910_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI62699.1"
                     /translation="MHIGYRRLKGRKGRPAVAGTWVARHYVGGRSYIVEKIGVADDFS
                     DADGTIILSFAQAQDKARTRMVLRARSAGGITGPLTVRAAVEGYLEFLEAHGKSAVDA
                     RHRANAHICPKLGDVEVSSLTPTMLRKWLANVARALPRARTRPGKPQQHRAFDGSDEA
                     IRRRKSSANRTWTILRAALNHAFHDDLVASDAAWRKVKAFRGVDAARIRYLTVAEARR
                     LINACAPEFRPLVQAALQTGCRYGELCRLEVTDFNRDAGTLAIRKSKSDKARHVVLTD
                     EGAEFFKGLTAGRAGNEPMFGRQWGISHQLRPMAEA"
     gene            complement(1365..>2246)
                     /locus_tag="VM910_08960"
     CDS             complement(1365..>2246)
                     /locus_tag="VM910_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62700.1"
                     /translation="RPVRWIVPYPPGGAADPIARLIGPFLSERLGKPVVIENKPGAGA
                     NIGTEFVVKSPPDGHTLLFITTANMINATFYRDLSFDFMRDITPVAGLVRLPLVLEVN
                     PSVPVKTVAELIAYAKANPGKVNFASAGVGTSLHLAAELFNMMTGTKMIHVPYRGSAP
                     ALTDLLAGQVQVLFDNLFTSLEHIKAGKLRALGVATAARVQQLPDLPTVADAVPGYEA
                     SSVFGVGVPTGTPREITELLNRELDAALADPRIRGRLLELTAIPIPSLAAEFRAEMVA
                     TTEKWGRVIRVANIKAE"
BASE COUNT          425 a          674 c          716 g          431 t
ORIGIN      
        1 cggcttcagc catcggacga agctgatgcg aaatgcccca ctgcctgccg aacatcggtt
       61 cattcccggc gcgcccggca gtgagtcctt tgaaaaattc agcgccttca tccgtgagta
      121 cgacgtggcg ggctttgtcg ctcttgctct tgcggatcgc caacgtgccg gcgtctcggt
      181 tgaaatcggt gacttcaagg cggcatagct cgccgtaccg gcagccggtt tgcagcgccg
      241 cttgcaccag ggggcggaat tcgggagcgc aagcgttgat gagccgccgg gcctccgcga
      301 ccgtcagata gcgaatacgc gccgcgtcga cgccgcggaa ggccttcact ttgcgccaag
      361 cggcgtccga cgcgacaagg tcatcatgaa aggcgtggtt gagtgcagcc cgcaagatcg
      421 tccaagtgcg attcgccgac gacttgcggc ggcggatggc ttcgtcggat ccgtcgaacg
      481 cacggtgctg ctgcggcttg ccaggcctgg tgcgcgcacg cggcagcgct ctggcaacat
      541 tggccagcca tttgcgcagc atggtcggcg tcagtgatga tacctcaaca tcgccgagtt
      601 ttgggcagat atgcgcattg gcgcggtggc gcgcatccac agcgctcttg ccgtgcgctt
      661 ccaggaattc gagatagcct tcgacggcag ctcgcaccgt cagtggccct gtaatgccac
      721 cggcagaccg cgcacggagc accatgcgcg tgcgagcctt gtcttgcgcc tgcgcgaaac
      781 tgaggatgat cgtcccgtcg gcatcactaa agtcgtctgc gacgccgatc ttctccacta
      841 tgtagctccg gccgccgacg tagtggcgtg cgacccatgt tcctgcgaca gcgggccttc
      901 ccttgcggcc tttcagcctg cggtagccga tatgcaagcc aggctcgagc agccggtagt
      961 gtggtttgcc cccgttcgag tcccctaggg agcgccaata atttcaagac gttatgagtg
     1021 atttgcttaa taggcgccat cgcgtacggc agaaatacgg catagacggt gctccgactg
     1081 gatgggggcg atgttagtaa aggctgcggc gaatagacgg ttgtgcggtg acccagcggc
     1141 gagggcagta gggacatcgt tttccgctaa ctcgggaaaa atcgggccgg acggactggg
     1201 tcgaaaaaat tctagatggc gcgggtattt tcaaatttcc tggagcgctt agcggaccca
     1261 gagcaagccg gcacctttcc caatagattg gcaatgcggc cagcccgaag gtcccgcctc
     1321 gatttcgctt aacgggagat tatggaatat cgggcatcgg cggattactc cgccttgatg
     1381 ttggccaccc ggatcacccg gccccatttt tcagtcgtgg caaccatttc agcacggaac
     1441 tcggctgcca ggctgggaat cggaatcgcc gtgagctcca gcagccgccc cctgatccga
     1501 gggtccgcca gcgcagcgtc gagctcccga ttcagcagct cggtaatttc gcgcggtgta
     1561 ccggtgggca cgcccacacc aaagacggag ctcgcctcgt aacccggcac ggcgtcggcg
     1621 accgtcggga gatcgggcaa ctgctgtacg cgggcggctg tcgccacacc gagcgcgcgc
     1681 agcttaccgg ctttgatgtg ctccagcgac gtgaacagat tgtcgaacag gacctgaacc
     1741 tgtcctgcaa gcaaatcggt gagcgcgggc gccgacccac ggtagggcac atgaatcatt
     1801 ttggtgccgg tcatcatatt gaacagctcg gcggcgaggt ggagcgaggt acccacgccc
     1861 gccgacgcga aattgacctt gcctggattg gccttggcgt aggcaatgag ttcggcgact
     1921 gtcttgaccg gaaccgatgg attgacctcc agcaccagcg gcaaccggac cagcccggcg
     1981 accggggtga tgtcccgcat gaagtcgaat gagaggtccc ggtagaacgt tgcattgatc
     2041 atattcgcag tggtgatgaa aagcagcgta tggccgtcgg gcggcgactt gaccacgaac
     2101 tcggtaccga tattggcgcc tgctccaggc ttgttttcga tcacgaccgg cttgcccagg
     2161 cgttccgata gaaacgggcc gatgagtcgc gcgatcgggt cggctgcgcc tccgggtgga
     2221 tagggaacga tccagcgcac cggccg
//
LOCUS       DATLJT010000391         5377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2319971,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000391 DATLJT010000000
VERSION     DATLJT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319971"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1420
                     /locus_tag="VM910_08965"
     CDS             227..1420
                     /locus_tag="VM910_08965"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI62701.1"
                     /translation="MHNQAEIDQVLRQKTDAREIPGVVAIAANSKDVIYQGAFGKRDL
                     SKDTAMTSNSVFWIASMTKAVTTAAAMQLVEQGKLALDSPIGKLLQDLAAPQVLEGFD
                     GAGEPKLRPAKGPITLRQLMTHSAGFCYSLWNGDMAKYIEKTGLPAITTCKNDALKLP
                     LMTEPGTRWEYGINIDFVGKAVEAASGKRLDAYLRDHLFKPLGMSDTSFKITESQRKG
                     LVGMHARGPDGSLAPIPFELEQDPEFHMGGGGLYSTAGDYIKFTQMILNKGKGNGNQV
                     LKPETVALMGQNQMGELSVTKMISADAALTNDVDLYPDQVKKWGLSFLINTAKTAEGR
                     SAGSLAWAGLANTYFWIDPSRDVAGVILMQLLPFADGKCLEAFAGFEHGIYAGLDRAG
                     GKKAA"
     gene            complement(1426..2427)
                     /locus_tag="VM910_08970"
     CDS             complement(1426..2427)
                     /locus_tag="VM910_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HVI62702.1"
                     /translation="MADAKYDVLGIGNAIFDVLVKTDEGFLASHGMTKGSMALIDEQR
                     AAAIYRDMGPATEMSGGSAANTIVGLASLGARTAYVGKIRDDQIGRMYAHDIRAAGVA
                     FETRPASNGPATGCSYILVTGDGERTMNTYLGAAQDLSPADIDPNEIAGARIVYLEGY
                     LWDPKDAKDAFVKASKIAHQADRQVALTLSDTFCVDRYRGEFVDLMRSGTIDLVFANE
                     AELHSLYQTSDFDTALKQLRDDAKLGVVTRSEKGCVVASRDGVIAVPAFPIEEIVDTT
                     GAGDLFAAGFLFGLVRDAGYENAGRLGALAAAEVIQHIGARPQVSLKELARKHGLPV"
     gene            2498..4054
                     /gene="murJ"
                     /locus_tag="VM910_08975"
     CDS             2498..4054
                     /gene="murJ"
                     /locus_tag="VM910_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVI62703.1"
                     /translation="MIRSFLTVSSGTLASRLLGFLRDTMIAGLLGTGAVADAFLVAFQ
                     FVNVVRRLLTEGALNAALVPAWLRLRETGGANEAARFAGRVLGTVSAGLVAGTVLIGL
                     LMPVIVAVLAPGFAGHQTLQLAVDDARLMLPYLAFAGPSTVLLALASAQGRFLLTAFA
                     PLLFNIALIVVMIVLIFARKDAGFVATALAATIGIAGLLQLLILVLRRQDETVARPVR
                     VSFDPEMRSFLGRALPGMVASSGPQLLMVAGAIIASSVPSAVSWLYFANRLVELPLGI
                     VGVAMGTVLIPELTRAVRGGDRTAIAHAESRALELAVGLALPAMLGLIVLSEPIVRLL
                     FEHGAFTPVDRAATSQALVWLALGLPAQVLVKAISPAFFAREDTFAPLVATSKALVVA
                     IVSALLLGHVFGASGIAAAIALGAWSNAISLIRQGGATFGFSLDPASRQRLARVIAAA
                     LAMGGLLWLTATFAAIPTATSHGLAQAIVLVVLIAGAIAVYAALLAAFGITGWRETMA
                     ALRRSPDLPA"
     gene            4132..5193
                     /gene="trpS"
                     /locus_tag="VM910_08980"
     CDS             4132..5193
                     /gene="trpS"
                     /locus_tag="VM910_08980"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVI62704.1"
                     /translation="MAFVERVFSGVQPTGNLHLGNYLGAIVNFVKLQQTHNCIYCVVD
                     LHAITQPTDVWGGPAELARNTREVTSAFIASGIDPKKHIVFNQSQVSGHAELAWIFNC
                     VARVGWLNRMTQFKEKAGKDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLE
                     LSRDIAQKFNNDFVDSIRRHGLNDGLFFPLPEPLITGPATRVMSLRDGTKKMSKSDAS
                     DYSRINLTDDADTIAQKIRKAKTDPEALPTEEKGLETRPEADNLVGIYAALAGRSKQD
                     VLREFGGGQFSSFKNSLVELCVSKLAPIAAEMKRLAADPGHVDAILVDGAERARKIAH
                     ETMQKTKDIVGFVHKHDAR"
BASE COUNT         1001 a         1688 c         1678 g         1010 t
ORIGIN      
        1 agtgcgaaac cagcatatcg ccgatgcctt gcagagtcat cggcgattcc ccggcagatt
       61 atggcaagcg cggcggttct gtatgccgtt aaccgtgttt tcccctgccg tttgctccga
      121 tcacgagatt gcgaccttcg atcgcagctc cgcctcggct gcttgcggga gagagcgagc
      181 cggaggaaga tcgccgccac ctgaaaaaat tagcgggagg agaccggtgc acaaccaagc
      241 cgagatcgac caggtgctcc gacagaagac cgacgcgagg gagatcccgg gcgtggttgc
      301 gatcgccgcg aacagcaagg acgtgatcta tcagggcgcg ttcggcaagc gcgatctgtc
      361 caaggacacc gcgatgacat cgaacagcgt gttctggatc gcttcgatga ccaaggccgt
      421 cacgaccgcc gcggccatgc agcttgtcga gcagggaaag ctcgcgctgg attccccgat
      481 cggcaagctg ctgcaggacc tcgcggcgcc gcaggtgctc gaaggctttg acggcgcagg
      541 cgagccgaag ctgcgccctg ccaaggggcc catcacgctg cgtcagctca tgacccacag
      601 cgccggcttc tgctacagcc tgtggaacgg cgacatggca aaatacatcg agaaaacggg
      661 gcttcccgcc atcaccacct gcaagaacga cgcgttgaaa ctcccgctga tgaccgagcc
      721 cggcacgcgc tgggaatacg gcatcaatat cgatttcgtg ggcaaggccg tggaggctgc
      781 gagcggcaag cggctcgatg cctatctgcg ggaccatttg ttcaagccgc tcggcatgag
      841 cgacaccagc ttcaagatca cggagtcaca gcgcaaggga ctggtcggca tgcatgcgcg
      901 agggccggac ggatcgctgg caccgatccc gttcgagctt gagcaggatc cggaattcca
      961 tatgggcggc ggcgggctct acagcaccgc aggcgactat atcaagttca cccagatgat
     1021 cctcaacaag ggaaagggca acggcaacca ggtgctgaag ccggagaccg tcgcgttgat
     1081 ggggcagaac cagatgggcg agctcagcgt gaccaagatg atctcggcgg acgcagctct
     1141 caccaacgac gtcgatctct atccggatca ggtgaagaaa tggggcctga gcttcctgat
     1201 caacacggcg aaaacggcgg aaggacgcag cgcgggcagc ctcgcctggg cgggattggc
     1261 caatacctat ttctggatcg acccgtcgcg ggatgttgcc ggcgtgatcc tgatgcagct
     1321 cctgcccttc gcggacggga agtgtctcga agcttttgcg ggctttgaac acggcattta
     1381 tgcggggctc gatcgggccg gcgggaaaaa ggcagcgtaa ggacgctaca ccggcaaacc
     1441 gtgtttcctt gcaagctcct tcagcgagac ttgcggccgc gcgccgatat gctgaatgac
     1501 ttcggctgcc gcgagcgcgc cgagcctgcc ggcattttca tagcccgcat cgcgcaccag
     1561 tccgaacagg aacccggcgg caaagagatc gcccgcgccg gtggtatcca cgatctcctc
     1621 gatcggaaac gccgggaccg cgatcacgcc gtcccgtgag gcgaccacgc agcccttttc
     1681 actgcgggtc acgacgccga gcttcgcatc gtcgcgaagt tgcttcagcg cagtgtcgaa
     1741 atcggaggtc tgatagagcg aatgcagctc ggcttcattg gcgaagacaa ggtcgatcgt
     1801 gccgctgcgc atcagatcga cgaactcccc gcgatagcgg tccacgcaaa acgtgtccga
     1861 cagcgtaagc gcaacctgcc gatccgcctg atgcgcgatc ttcgaggcct tcacaaaagc
     1921 gtctttggca tccttcggat cccagagata accttcgaga taaacgatcc ttgctcccgc
     1981 aatttcgttc ggatcgatat cagccggcga cagatcctgc gcggcaccga gataggtgtt
     2041 catggtgcgc tcgccgtcgc cggtcacgag aatgtaggag cagccggtcg cgggaccatt
     2101 ggaagcgggt ctggtttcga aggcaacgcc tgcggcgcga atgtcatggg cgtacatccg
     2161 gccgatctgg tcatccctga tcttgccgac ataggcggtg cgggcgccca ggctcgccaa
     2221 tccgacgatg gtgttggctg ccgagccgcc ggacatttcg gtggccggcc ccatgtcgcg
     2281 atagatcgca gccgcgcgct gctcgtcgat cagcgccatg ctacccttgg tcatgccgtg
     2341 gcttgcaagg aagccctcgt cggtcttaac cagcacatcg aaaatcgcat tcccaatccc
     2401 gagaacgtcg tattttgcgt cagccattga tccgtcctgt tgcggccatg aacgaggctc
     2461 gcggcctatc acagccaacg ctgccataga agccccaatg atccgctctt tcctcacggt
     2521 atcttccggc acgctcgcct cgcggctgct cggattcctt cgcgatacca tgatcgcggg
     2581 cctgctcggc accggcgcgg tcgcggacgc cttcctcgtg gcctttcaat tcgtcaatgt
     2641 ggttcggcgg ctgttgacgg aaggcgcgtt gaacgcggct ctggtgcccg cctggctgcg
     2701 cctgcgcgaa accgggggcg cgaatgaagc ggcgcgcttc gccggacgcg tactcggcac
     2761 cgtcagcgcc ggtcttgtgg cggggacggt gttgattggc ttgttgatgc cggtgatcgt
     2821 cgcggtgctg gcgcccggct ttgccggaca tcagaccctg cagctcgcgg tcgacgatgc
     2881 gcgcctgatg ctgccctatc ttgccttcgc tggcccctcg acagtgttgc tcgcgctggc
     2941 gagcgcgcag ggccgatttc tgctcaccgc ctttgccccg cttctgttca acatcgcgct
     3001 gattgtcgtg atgatcgtgc tcatttttgc acgcaaggat gccggcttcg ttgcaacagc
     3061 acttgctgca accatcggca tcgcgggcct gctgcaattg ctgattctcg tcttgcgccg
     3121 tcaggacgaa acggtcgcgc ggccggtgcg ggtctcgttc gatcccgaga tgcgcagctt
     3181 ccttggacgc gctctcccgg gcatggtcgc aagctcgggg ccgcagcttc tgatggttgc
     3241 gggcgcgatc atcgcctcgt ccgtgccctc ggcagtatcg tggctctatt tcgccaaccg
     3301 cctggtcgaa ctgccactcg gcatcgtcgg cgtcgcgatg ggcacggtgc tgataccgga
     3361 attgacgcgc gcggtacgcg gcggtgacag gaccgcaatc gcgcacgcgg aatcgcgtgc
     3421 actcgaactc gcggtgggat tggcgctgcc ggccatgctt ggcttgatcg tcctgagcga
     3481 gccgatcgtg cgcctgttgt tcgaacatgg cgcgttcacc ccggtcgatc gcgcggcgac
     3541 ctcgcaagca ttggtctggc tcgcgcttgg cctgcccgcg caggtgctgg tcaaggcgat
     3601 atcgccggcc tttttcgcgc gcgaagatac gtttgcccct ctcgttgcga catcaaaggc
     3661 ccttgtcgtc gccatcgttt ctgccctgct gctgggccat gtgttcggcg cgagcggcat
     3721 cgccgcggcg attgcgctcg gcgcctggag caacgcaata tccctgatcc gacagggcgg
     3781 cgcgaccttc ggcttttcgc tcgatccggc gtcgcggcaa cggctggccc gcgtcatcgc
     3841 ggcggcgctc gcaatgggcg ggttattgtg gttgaccgcc actttcgccg cgatcccaac
     3901 cgcaaccagc catggactcg cccaggcaat cgtcctggtt gtcttgattg ccggggcgat
     3961 cgcagtttac gccgcgctgc tggcagcctt cggcatcacc ggctggcgcg agaccatggc
     4021 tgcactccgg cggagcccgg acctgcccgc ctgaagacgc gctttgccct tgacggcgca
     4081 gggcagctct tggcaaacga gcgccaattg atttggaacg ggaaatcgac gatggccttc
     4141 gttgaacggg ttttttcggg cgtccagccg accggcaatc tgcatctcgg caattacctc
     4201 ggcgcgatcg tgaacttcgt caaattgcag cagacgcata attgcatcta ttgcgtcgtc
     4261 gacctgcatg cgatcacgca gccgaccgac gtatggggcg ggccggccga gcttgcacgg
     4321 aacacccggg aagtgacgtc cgccttcatc gcaagcggca tcgatccgaa gaagcacatc
     4381 gtcttcaacc agagccaggt ctccggccac gccgagcttg cctggatctt caactgcgtc
     4441 gcccgcgtcg gctggctcaa ccgcatgacg cagttcaagg agaaggctgg caaggatcgc
     4501 gaaaatgcgt cggtgggtct ttacgactat ccggtgctga tggcggccga catcctgctt
     4561 taccgcgcca cccacgtgcc cgttggcgag gaccagaagc agcatctgga gttgtcgcgc
     4621 gatatcgcgc agaaattcaa caacgacttc gtcgactcga tccgccgtca cggcctcaat
     4681 gacggcttgt tctttccgtt gcccgagccc ctgatcacgg gtcccgcgac gcgcgtgatg
     4741 agcttgcgcg acggaacgaa aaagatgtcg aagtcggatg catcggatta ttcgcgaatc
     4801 aacctgaccg acgatgccga caccatcgcg cagaagatcc gcaaggcgaa gaccgatccg
     4861 gaggcgctgc cgaccgagga gaaaggactg gagacgcggc ccgaggccga caacctggtc
     4921 ggcatctatg ccgcgcttgc gggccgcagc aagcaggacg tgctgcgcga attcggcggc
     4981 ggacagtttt caagcttcaa gaattcgctg gtcgaactct gcgtctcaaa actggcgccg
     5041 atcgccgccg agatgaagcg attagcggcc gaccccggcc atgtcgacgc gatcctcgtc
     5101 gatggtgccg agcgcgcccg gaaaattgcg catgagacca tgcaaaagac gaaagacatc
     5161 gtgggcttcg tgcacaagca cgacgcccgc tgacgcctgc tgttgcagtg cacggtgccg
     5221 cacacagtat ttcccccaca aaggtgacct ctcgcaaagc aaggggcctt gtggcaccat
     5281 gacggtcgcg cttgcgcagc agcgggacat gcatgagtac ccagcgacga agctacgaga
     5341 cgggtcacaa gccgaaatgt ctggttatcg ttgacga
//
LOCUS       DATLJT010000392         2947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3698642,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000392 DATLJT010000000
VERSION     DATLJT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698642"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..731
                     /locus_tag="VM910_08985"
     CDS             345..731
                     /locus_tag="VM910_08985"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HVI62705.1"
                     /translation="MSDETRLYYLRIALIVFGLIFVFAVYPLTRVWPSGWSWGMGHSH
                     YLMMILGIYATLGVFLLIASRDPLANRSLIWFTVWSSIVHGAIMAAQAIRDPTEVGHL
                     IGDVPALFLIAIVLAALMPRAAPARA"
     gene            complement(728..1897)
                     /gene="pobA"
                     /locus_tag="VM910_08990"
     CDS             complement(728..1897)
                     /gene="pobA"
                     /locus_tag="VM910_08990"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HVI62706.1"
                     /translation="MRTKVAIIGAGPAGLLLGHLLKTYGIDNVILERHTPDYVLGRIR
                     AGLLEEGTVRLLDEVGAGSRAHAEGLVHDGIELAFDGARHRIDMKALTGKTVMIYGQT
                     EVTLDLMNARKAAGLTSIYEAADVQPLDFDTDHPRVTYVRDGATHEIACDFIAGCDGF
                     HGISRASVKADAIETFERIYPFGWLGILSETPPVSHELIYSNHARGFALCTMRSTHRS
                     RYYVQCPLDDHIDQWPDERFWDELKSRLDQRAVDKLVTGPSIEKSIAPLRSFVAEPMR
                     FGRMFLAGDAAHIVPPTGAKGLNLAASDAHYLAVSLREYYDEKSSAGIDHYSSTALTR
                     VWKAVRFSWWMTSMLHKFPDNGRFGARIQIAELDYVTSSKAAMTSLAENYVGLPF"
     gene            2043..>2947
                     /locus_tag="VM910_08995"
     CDS             2043..>2947
                     /locus_tag="VM910_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI62707.1"
                     /translation="MRKTLLALLVAASVTPALAQEKNIDLKISHWVPASHPLQKSLED
                     WAAAVEKASGGTIKSQVFPAQQLGKAFDHYDMARDGIADVTYVNPGYQPGRFPIIGAG
                     ELPFLMSDAKGGSEALDAWYRKYADKEMKDVKFCLAFIHSPSSFHTRTKKIMVPEDVK
                     GMKIRPAHATMANFVTLLGGTNVQSSAPEVRDIIERGVADGVTFPWGSLVLFGVDKVT
                     KYHMEAPLYVTTFVVVMNKDKYNQMSDRQKKAIDDNCNTQAAGRVGEPWGKFEDAGVD
                     KVRAEPGHEVYTLTAEQTALWKKAA"
BASE COUNT          570 a          905 c          912 g          560 t
ORIGIN      
        1 acccctcggg aatatcggtt gcggtcatgc tgatctcgcg cgggtcacgt ctcgtctcat
       61 tgaacgcgat ggacgcgcgg atttcacggc ctacaaagtt ttaaacggaa tttgcacggg
      121 tgtttgaaag caaggtcgca agggcgatcg tgtgtcaggc cggcaattca tagccgatcg
      181 aaggcgcacc cacctcacgc aatgcaatat gtgccgccat cgagatcgag cccggagcga
      241 aatagcgggt gagcatgcgg cagcttccgg ctgtgcttgt ctggagcgca ctctctgata
      301 acgttcatgc ttcgtctacg caccgacgga agggggaggg agcaatgtcg gacgaaaccc
      361 gcctgtacta tcttcgcatc gcgctgattg tcttcggact gatctttgtc tttgccgtct
      421 atcccctgac ccgcgtctgg ccgtcgggtt ggtcatgggg aatgggtcat tcgcactatc
      481 tgatgatgat cctcggcatc tatgcgacgc ttggcgtctt cctgctgatc gcgtcgcgcg
      541 atccgctcgc caatcgcagc ctgatctggt tcacggtgtg gtccagcatc gtccatggcg
      601 ccatcatggc cgcgcaggcg atcagggacc cgacggaggt cggccatttg atcggcgacg
      661 ttccggcgct gttcttgatt gcgatcgtgc tggccgcgct gatgccgcgc gccgcgccgg
      721 cacgcgccta gaacggcagc ccgacgtaat tttccgcaag tgacgtcatt gccgccttcg
      781 agctcgtgac atagtccagc tcggcgatct ggatgcgcgc gccgaaccgc ccgttgtccg
      841 gaaacttgtg cagcatcgac gtcatccacc aggagaagcg cacggccttc cagacccgcg
      901 tcagcgcggt ggacgaataa tggtcgatcc cggcagacga tttctcgtcg taatattcgc
      961 gcaggctgac cgcgagataa tgcgcgtcgc tggcggcaag gttcagtccc ttggcgccag
     1021 tcggcggcac gatatgggcg gcgtcgcccg ccaggaacat ccggccgaag cgcatcggct
     1081 cggcgacgaa gctgcgcaag ggcgcgatgc tcttctcgat cgaggggccg gttacgagct
     1141 tgtcgaccgc cctctggtcg agcctgctct tcaattcgtc ccagaaccgc tcgtcaggcc
     1201 actggtcgat gtggtcgtcg agcgggcact gcacgtaata gcggctgcgg tgcgtcgagc
     1261 gcatggtgca gagcgcaaag ccgcgcgcat gattggaata gatcagttcg tgcgagaccg
     1321 gcggcgtctc cgacaaaata ccgagccagc cgaacgggta gatgcgctca aaggtctcga
     1381 tcgcgtcagc tttgacgctg gcgcggctga tgccgtgaaa gccgtcgcaa cccgcgatga
     1441 aatcgcaggc gatctcatgg gtcgcaccat ccctgacata ggtgacgcgc ggatgatcgg
     1501 tgtcgaaatc gagcggctgc acgtcggccg cttcgtagat cgacgtcaaa ccggctgcct
     1561 tgcgcgcatt catcagatcg agcgtcacct cggtctggcc gtagatcatc acagtcttgc
     1621 cggtcaacgc cttcatatcg atgcgatgac gcgcgccgtc gaacgcaagc tcgatgccgt
     1681 catgcacgag accttccgcg tgcgcgcggc tgccggcgcc gacttcatcc agaagcctga
     1741 ccgttccttc ttcaagcagc cccgcgcgga tgcggccgag cacgtagtcc ggcgtgtggc
     1801 gctccaggat gacgttgtcg atgccgtagg tcttgagcag gtgccccagc agcaatcctg
     1861 ccggtcccgc gccgatgatt gcgacttttg tccgcaatac ttgctccccc cggtagtata
     1921 gggttcaagg tccgccgccg cgctttgtcg cgggcttgcc atgataatta tatcatataa
     1981 caatatctgc aaagggcgcg taagcctgaa agaaacctga aagaaaaagg gagacgatac
     2041 cgatgaggaa gacgcttctg gcgctgctgg tggccgccag cgtgacgccg gcactggcgc
     2101 aggaaaagaa catcgatctg aagatctcgc attgggtgcc tgcctcgcat cccctgcaga
     2161 agtcgctgga agactgggcc gccgcggtcg agaaggcgtc cggcggcacc atcaaatccc
     2221 aggtcttccc cgcccagcag ctcggcaagg cgttcgacca ttacgacatg gcgcgcgacg
     2281 gcatcgccga cgtcacctat gtcaatcccg gctatcagcc cggccgcttc ccgatcatcg
     2341 gcgccggcga actcccattc ttgatgtcgg acgccaaggg cggctcggag gcgctggatg
     2401 cctggtaccg gaaatatgcc gacaaggaga tgaaggacgt caaattctgc ctcgccttca
     2461 tccattcgcc ttcgtcgttc cacacccgca ccaagaagat catggtgcct gaggacgtca
     2521 agggcatgaa gatccggccc gcgcacgcca ccatggcgaa tttcgtgacg ctgctcggcg
     2581 gcaccaatgt gcagtcctcg gcgccggaag tacgcgacat catcgagcgc ggggtcgccg
     2641 acggcgtcac gttcccctgg ggatcgctgg tgctgttcgg cgtcgacaag gtcacgaagt
     2701 atcacatgga ggcgccgctc tacgtcacga ccttcgtcgt cgtgatgaac aaggacaagt
     2761 acaaccagat gtccgaccgc cagaagaagg cgatcgacga caattgcaac acgcaagccg
     2821 caggccgcgt cggcgaaccc tggggcaagt tcgaggatgc cggcgtcgac aaggtgaggg
     2881 cggagccggg ccatgaggtc tacacgctga cggcggagca gaccgccttg tggaagaagg
     2941 ccgccga
//
LOCUS       DATLJT010000393         5169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_482095,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000393 DATLJT010000000
VERSION     DATLJT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482095"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VM910_09000"
     CDS             <1..628
                     /locus_tag="VM910_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62708.1"
                     /translation="PLRERGSELLPLIATIGVSILLQNAMLLWFGPIPYAFDSPYSNK
                     VIRLWGGTFITLQNLIIVLVSALTIVLLYAFMKFTMMGKALRAVAQDRETAGLMGINP
                     SQLIMLTFVIASALAGMGGAMLGPILVLTPFAGTSVIVKAFAIVIIGGFGNVEGTIIA
                     GLLVGLIESYTTQFVNPGLTDIVVFTLLLLMLALRPTGLIAEKREENV"
     gene            621..1655
                     /locus_tag="VM910_09005"
     CDS             621..1655
                     /locus_tag="VM910_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI62709.1"
                     /translation="MSNEDRPALLVLPDQIRINRRGWFLVVAVIIALLPFVIQDSYWR
                     TNLIVCAINVMLAIGLDFILGYAGQLNLGHSAFYGIGAYASTLLIMKLGVPFWMAFVF
                     GMVLSGAAGMALSIFAVRLRGHYLAIASLGFAVIVHQVLLNWISLTQGPLGIYAIKPP
                     PDIALTGLSVISFANTANMFYLVAGFAFSFYLLLDQLVRSPIGETLTAIREDEISASS
                     FGINCTAWKVFAFGIGSALAGAAGAFYASFVGTLVPDAFIITESFTILAMVIVGGMGT
                     LIGPVWGAILLTMLPELLRGFGDLRLVLYGAALTLVVLFMPGGIVQAAQIFRARLGRL
                     ATATDAGMRR"
     gene            1652..2464
                     /locus_tag="VM910_09010"
     CDS             1652..2464
                     /locus_tag="VM910_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62710.1"
                     /translation="MIAPGSAAVAQARPTAAGAFFFGAGLHKRFGGVQAVRDISLNVP
                     RGGVFAIIGPNGAGKSTLLNLMSGLYQPDAGRMIFDGVDLVGLQAHKRVRLGLARTFQ
                     KLRLFKQLSVLGNVIAASHIHHDIPAWQYVVHAAAFGRDHARCRAQAIKLLGFVGLEG
                     RASVQAASLAYGEQRMLELARALATSPRLLMLDEPAAGLNAAEVERLLERIRLLREDG
                     MTIIVVEHNMDLVMKVSDRVLVMDYGQHLFEGTPAEVQANPGVIAAYLGAMT"
     gene            2470..3201
                     /locus_tag="VM910_09015"
     CDS             2470..3201
                     /locus_tag="VM910_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62711.1"
                     /translation="MLQAEGLQLAYGEISACRDVSLHVGQGEIVTLIGANGAGKSTTL
                     RAVAGALLPRAGSIHFRGYDITRLPSYERTKLGISLVPEGRRVFPFLTVRENLELGAF
                     KYRKEGAKVRSLMDKVFDMFPRLRERRSQNAGTLSGGEQQMLALGRAIMSEPHLVCLD
                     EPSLGLAPMVVQDIFRTIKAINAAGTSVLLVEQNARYALETASRGYVLQTGSIIASGP
                     CATLRNDDRVRQAYLGRSAAERMRL"
     gene            complement(3312..4190)
                     /locus_tag="VM910_09020"
     CDS             complement(3312..4190)
                     /locus_tag="VM910_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62712.1"
                     /translation="MPHLTTDDNVKLYYEDSGSGIPIVFVHEFAGDYRSWESQVRHFA
                     RGYQCITYNARGYPPSDVPADGERYSQDRARDDIRAVLDALKIEKAHIVGLSMGGFAA
                     LHFGFTYPQRARSLVVAGCGYGAAPNQRQQFADEAEAAARKFEETGMARAAEAYALGP
                     TRVQFQNKDPRGWKEFVDQLAEHSSEGSARTMRGVQKRRPSLFDLVDKMKTIAAPTLV
                     MTGDEDWPCLEPGLLMKRTIPTAGLVVMPNAGHTINLEEPAAFNQHLANFFQAVDAGA
                     WRSRDPRATKEAILGR"
     gene            4573..>5169
                     /locus_tag="VM910_09025"
     CDS             4573..>5169
                     /locus_tag="VM910_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62713.1"
                     /translation="MGRLSRYVAVALVASLKCIGPALSQEWPTRPVTMVVPFAAGGPA
                     DTVGRILAPGLSELLGQQVIIENVGGSGGMTGSSRVAKAAPDGYQFVLGNVGTHAANQ
                     TFYKTPLYNAATDFAPVMLIAQTPLVLLARKDLPANNLKEFIAYAKVNQASMQYGSGG
                     AGSASHLACVLLNAAIGINVTHVPYRGAAPAMQDLIAGR"
BASE COUNT          889 a         1594 c         1561 g         1125 t
ORIGIN      
        1 gccgctgcgc gaacgcggca gcgaattgtt gccgctgatc gccaccatcg gcgtgtccat
       61 tctgttgcag aacgcgatgc tgctctggtt cgggccgatc ccttatgcct tcgatagccc
      121 ctacagcaat aaggtcatcc ggctctgggg cggcacattc atcacgttgc aaaatctcat
      181 catcgtcttg gtatccgccc tcaccatcgt gctgctctac gcattcatga aattcaccat
      241 gatgggcaag gcgctgcgcg cggtcgcaca ggatcgcgag accgcgggcc tcatgggcat
      301 caatcccagc caactcatca tgctgacctt cgtcatcgcc tcggcgctcg ctggcatggg
      361 cggcgccatg ctcgggccca tcctcgtgtt gacgcccttt gccggtacga gcgtaatcgt
      421 gaaggctttt gccatcgtga tcataggcgg gttcggcaat gtggagggaa caattattgc
      481 aggactcctg gttggtctga tcgaaagcta cacgacccag ttcgtcaatc ccggactaac
      541 tgacatcgtg gtcttcacgc tgctgcttct gatgctcgca ttacgtccga caggactcat
      601 cgccgaaaag cgtgaagaga atgtctaacg aggatcgacc ggcgcttctc gtgctacctg
      661 atcagatccg aattaaccgg cgaggatggt tccttgtcgt cgccgtgatc atcgctctct
      721 tgccgttcgt cattcaagat tcttattggc gcacgaacct gatcgtttgc gcgatcaacg
      781 tcatgctggc tatcggcctt gatttcatac tcggctacgc cggccagctt aatctcggtc
      841 actcggcgtt ctacggcatc ggcgcctatg cctccacact tctcatcatg aagcttggcg
      901 tgccgttctg gatggcgttc gtcttcggaa tggtattgtc cggcgccgcc ggcatggcgt
      961 tgtcgatctt cgccgttcgt ctgcgcggcc attatcttgc catcgcctcg ctcggctttg
     1021 ccgtcatcgt gcatcaggtg ctgctgaact ggatcagcct gacgcaaggc ccactcggca
     1081 tctatgccat caagccgccg ccggacatcg cgctgacggg gctgtcggtc atttccttcg
     1141 ccaacacggc gaacatgttc tatctggtcg cgggcttcgc attctccttc taccttctgc
     1201 tcgaccagtt ggtccgctcg ccgatcggcg agaccctcac ggcgatcaga gaggacgaga
     1261 tttcggcttc ctcgttcggc atcaattgca cggcatggaa ggtgtttgcc ttcggcatcg
     1321 ggtcggcgct cgccggcgct gccggcgcct tctatgcgag cttcgtggga acgttggttc
     1381 cggacgcctt tatcatcacc gaatccttca cgattctcgc catggtgatt gtcggcggca
     1441 tggggacgct gatcggccca gtctgggggg cgatcctgct gacgatgttg cccgaacttt
     1501 tacgcggatt cggcgatctc cgtctggtcc tctacggtgc cgctctcacc cttgtcgtgc
     1561 tgttcatgcc gggagggatc gtgcaggcag cgcagatctt ccgcgcgcgg ttgggtcggc
     1621 tagcaaccgc caccgacgct ggaatgcgcc gatgatcgcg ccaggctccg ctgcggtagc
     1681 gcaagcacgt cctaccgctg ccggcgcttt ctttttcggc gcaggcttgc acaaacggtt
     1741 cggcggcgtg caggcggttc gcgacatttc gctcaatgtc ccgcgtggcg gcgtgtttgc
     1801 gatcatcggc ccgaacgggg ccggcaagtc gacgctgctc aacctgatga gcgggctcta
     1861 tcagccggat gccggtcgga tgatcttcga cggcgtcgac ctcgtaggct tgcaggcgca
     1921 caaacgggta cgcctcggcc ttgcgcgtac attccagaag ttgcggcttt ttaagcagtt
     1981 atcagtgctc ggcaacgtca tcgccgcctc tcacatccat catgatatcc cggcttggca
     2041 atacgtcgtc cacgcggccg cgttcgggcg cgaccatgcc cgctgccgcg cgcaggcgat
     2101 caagctcctc ggcttcgtcg gactcgaggg acgcgcatcg gtgcaggcgg cttcgctcgc
     2161 ctatggtgag cagcgcatgc tcgagctcgc ccgcgcgctc gcaacctcgc ctcgcctgct
     2221 tatgctcgac gagcccgccg ccggcctcaa cgcggcggaa gtggagcggt tgcttgaacg
     2281 cattcgtctt cttcgcgagg acggcatgac catcatcgtt gtcgagcaca acatggatct
     2341 ggtgatgaag gtctcagatc gggtgctggt catggattac gggcagcatc ttttcgaggg
     2401 cacgcccgcg gaggtccaag ccaatccggg agtgattgct gcctatcttg gagcaatgac
     2461 gtgaccgcgc tgcttcaagc cgaaggactt caactggcct atggtgagat ttcagcctgc
     2521 cgcgatgtca gcttgcatgt ggggcagggt gagattgtca cgctgattgg cgccaatggc
     2581 gccggcaaat cgacgacgct gcgcgccgtc gccggcgcac ttttgccgcg tgctggctca
     2641 atccatttcc gcgggtacga catcacgcgc ctgccatcgt atgagcgcac caagctcggg
     2701 atttcactcg tccctgaggg ccggcgcgtg ttcccgttcc tcacggtgcg cgaaaatctc
     2761 gagctaggcg ccttcaagta ccgcaaagag ggcgcgaagg tgcgaagcct catggacaag
     2821 gtcttcgaca tgtttccgcg gctgcgtgag cgcaggagcc aaaacgctgg cacgctttca
     2881 gggggcgagc aacagatgct cgcgctaggg cgtgcaatca tgtctgaacc gcacctcgtt
     2941 tgtctcgacg agccctcgct cgggcttgcg ccgatggtcg tccaggacat tttccgcacg
     3001 atcaaggcga tcaatgctgc tggcacgagt gtgctcctgg tcgagcagaa cgcgcgctat
     3061 gcgcttgaga ccgcgagccg cggttatgtg ctgcagacgg gatcgatcat cgcaagcggc
     3121 ccctgtgcga ccctgcgcaa cgacgatcgt gtgcgccaag cctatctcgg ccgcagcgcg
     3181 gccgagcgga tgaggctatg acgcgcgatt tcttttggct cgacgtcgct cgcttcagga
     3241 gcggcggatg gacggcaatc gatttttgct acccgtttcc gcaagcctga cgagcggatg
     3301 catcgacctt gtcaccgtcc caaaattgct tctttcgtcg cacgcggatc acgagaacgc
     3361 caagcgccgg catcgaccgc ctggaagaag ttggcgagat gctggttgaa ggcggccggc
     3421 tcttcaaggt tgatggtgtg gccggcattg ggcatcacca cgagccctgc cgtcgggatc
     3481 gtccgcttca tgaggagccc gggttcgagg cacggccagt cctcgtcacc ggtcatgacg
     3541 agggttggag cggcgatcgt cttcatctta tcgacgaggt cgaacagcga cggccgccgt
     3601 ttctgaacgc cgcgcatggt gcgcgctgaa ccttcgcttg agtgctcggc gagctgatcg
     3661 acgaattcct tccagccacg cgggtctttg ttctggaact gcacgcgcgt tggtccgagt
     3721 gcataagcct ccgcggctct cgccatgccg gtctcttcga acttccttgc cgctgcctcc
     3781 gcttcgtctg cgaactgctg gcgctggttg ggcgcagccc catagccgca gcccgccacg
     3841 accagggaac gcgcacgttg tggataggtg aagccgaaat ggagtgccgc aaagccgccc
     3901 atggaaagcc cgacaatgtg agctttctcg attttcagcg cgtcgagcac agcgcgaatg
     3961 tcgtcgcgcg cgcgatcctg cgaataacgc tctccatccg ccggcacgtc cgaaggcgga
     4021 taaccccgtg cattgtaagt gatgcactga tagccgcggg caaaatggcg aacctgcgat
     4081 tcccacgaac gatagtcgcc ggcgaattca tgtacaaaga cgatcggaat gcccgatccg
     4141 ctgtcctcat agtagagttt cacgttgtcg tcggtggtca gatgcggcat cgatgccccc
     4201 tttgctcaag ctctgtcaca ttggtcaagt cgtcagcgat ggtagtcggg acgcgtgcgc
     4261 gccaatcgaa ggagattctc gacggccata aacgcgttga ggcttagttc gagccgccgg
     4321 acgcgctccg ctgcagtcat gctcattttt gcgacgcgca gttgcattgg gtgtcggcca
     4381 attcggaatg cgggcgtaaa ttggcgtcac gtttgaattt acccggaagt cgatgcggtc
     4441 gcgagcgacc gggcggctat agtcccgtgg cgtgacacac atccattttt cgacggatcg
     4501 ccggattgcg cacggactat ccaacgtccc ggcaactgtt attctcgtca tccaggtggc
     4561 ttggaggatt gaatgggacg gctatcccga tacgtggctg ttgcacttgt cgccagcctc
     4621 aaatgcatcg ggccggcgct atcgcaggag tggccgaccc ggccggtgac catggtcgtt
     4681 ccgttcgccg ccggcggtcc ggcagacacg gtcggacgca tccttgcccc gggcctttcc
     4741 gagcttttgg gtcagcaggt catcatcgaa aacgtcggcg gctccggcgg catgaccggc
     4801 agctcgcgcg tggcgaaggc ggcgcccgac ggctatcagt tcgtgctcgg caatgtcggc
     4861 acccatgccg caaatcagac gttctataaa actccgctct acaatgccgc gactgatttt
     4921 gcgcccgtca tgctgattgc gcagacgccg ctggtcctgc tcgcgcgcaa ggatttgccg
     4981 gccaacaatc tcaaggaatt catcgcctat gcgaaggtca accaagcgag catgcagtac
     5041 ggctcgggcg gagcgggttc cgcgagccat cttgcgtgtg tgttgttgaa tgcggccatc
     5101 gggatcaatg tcacacacgt gccctatcgc ggtgccgccc cggcgatgca ggacttgatc
     5161 gccggccga
//
LOCUS       DATLJT010000394         6694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3928205,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000394 DATLJT010000000
VERSION     DATLJT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928205"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VM910_09030"
     CDS             complement(<1..121)
                     /locus_tag="VM910_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HVI62714.1"
                     /translation="MRGVFSDQGRLFSYISPEARVPANHPLRKIRKLVRDVLGE"
     gene            complement(396..1295)
                     /locus_tag="VM910_09035"
     CDS             complement(396..1295)
                     /locus_tag="VM910_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HVI62715.1"
                     /translation="MDQTAGMVHLTTTLAHASGEWIASDWPVCPIAETANPQRMGAAL
                     TYARRYALFTLVGIAGEDDLDARISAMDHPHCCRRRLTARSSRRTRLAMDMAVVARKA
                     TSRSDSIPALREKLLAEVGNITSADLAAAWAGEALTAKNSLAAVDAKFVEDAFERRLS
                     ELPSSDAAAPSNDESSMPQIARARVTDPAEGAAPDQAKGIDKSVLTVAAPRRYRNRNT
                     CTTSPNRRVSCAAASPRTPTTSDLRRHARSAARSAMSSRSRSVEGITGRHMAHMTSAR
                     GGDKPALTRSKLPAGSGRRRTRT"
     gene            complement(1265..1513)
                     /locus_tag="VM910_09040"
     CDS             complement(1265..1513)
                     /locus_tag="VM910_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62716.1"
                     /translation="MPRSSETVAALASALAKAHAELINPEKSLTATIRTGRPGDGERS
                     FRYAPLSSGLDIVRKTLGSTGSPPYRRPPWIRPPAWSI"
     gene            complement(1497..2195)
                     /locus_tag="VM910_09045"
                     /pseudo
     CDS             complement(1497..2195)
                     /locus_tag="VM910_09045"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
     gene            2079..2453
                     /locus_tag="VM910_09050"
     CDS             2079..2453
                     /locus_tag="VM910_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62717.1"
                     /translation="MVLDVGNAPAGIALVPEAIELLGGGPELDNKVARQVLSLGLAPF
                     LLPETRALLRWSAAELAREAALGLATIKRAELADGETSMTMANDLAVRRAFEAAGVEF
                     IEENGGGPGVRLRKAHQHGKRK"
     gene            complement(2668..4089)
                     /locus_tag="VM910_09055"
     CDS             complement(2668..4089)
                     /locus_tag="VM910_09055"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HVI62718.1"
                     /translation="MVAQIIPVIMCGGAGRRLWPVSRESMPKQFVSLVGEGSTFQQVL
                     GRISEPDLFGRPIVITNTDFRFVVAEHLRGRGFEADIVLEPMGRDSGPAVAVSALLAA
                     QRDRDALVLVLAADHVVRKPDEFRQACQRAAAVAAEGRIVTFGIEPTHPATSYGYIRP
                     GEKLNGASARTVEAFVEKPDAATAANYVAEQYLWNSGNFLFHAATMLGEIECFEPAMA
                     EAAKAAVAGLTRDLDFLRLASEPFARAPKKSIDYAVMQRTKLAAVVPTTNMGWSDIGS
                     WSTVWDVLDHDAAGNATDGSVVMLDSRNSLVRSEEPVLTTVVGLDDVIVVSTADAVLV
                     SARAKAEQVKTLVEQLKAHNHRAAVEHRRVYRPWGYYQDVDIAPRYRVKRIVVKPGSK
                     LSLQKHFHRSEHWVVVKGTAEVTLGNHVRSVHENESIYIPIGSIHRLANPGKIPLELI
                     EVQVGSYLGEDDIVRLDDIYGRE"
     gene            complement(4646..5062)
                     /locus_tag="VM910_09060"
     CDS             complement(4646..5062)
                     /locus_tag="VM910_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVI62719.1"
                     /translation="MRQGEAVFVDSGAWIALALSRDPLHPQAREQWELLRGVGAKLHS
                     SVPVVIETFTFLDRNASRDVALTWKEAIYKPGTVRILPCELRDLEQSWEYFRRADLHK
                     LSAVDATSFAIMKRARIRLAYAFDHHFAVVGFSLVA"
     gene            complement(5062..5271)
                     /locus_tag="VM910_09065"
     CDS             complement(5062..5271)
                     /locus_tag="VM910_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVI62720.1"
                     /translation="MVKTQVYLRKEELDALRMAAARSGRSVAELIRGAIRKTLLKPQA
                     AGPVAIWDGEPKRTSIEHDSVHDGR"
     gene            5441..6426
                     /gene="galE"
                     /locus_tag="VM910_09070"
                     /pseudo
     CDS             5441..6426
                     /gene="galE"
                     /locus_tag="VM910_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
BASE COUNT         1274 a         2074 c         2051 g         1295 t
ORIGIN      
        1 gctctcccaa cacatcgcgc acaagcttcc ggatctttct cagcggatgg ttcgccggca
       61 cccgcgcctc aggcgaaatg tacgaaaaca accgaccctg atccgaaaat accccccgca
      121 tgcaagcctc cgccgattcg ctatagaaaa actgaatcag caaatccacg ctatggcgag
      181 gggtttttca gcaaactgct agtggaccga aaattgaaat cattgccaaa tgcgtgcatc
      241 ttggcactcc gggctgcgct caaatctttt ctcaaacaag ccggtttcgg tcgcactggc
      301 acgacgaagt ccgacaaaag gcttccagtc gcgctggttc tgctaatcag gaagatccga
      361 taagcgcgtt tcttcttgca acgttacggt ggcgctcatg tcctcgttct cctccttcca
      421 gagcctgcgg gcaactttga tcgggtcaat gccggcttgt cgccaccacg cgcgctcgtc
      481 atgtgagcca tgtgccgccc ggtgatgccc tcgacagagc gggaccgtga actcatcgct
      541 gaccttgcgg ccgagcgcgc gtggcggcgt aaatccgagg tggtgggggt ccgagggctt
      601 gcggccgcac acgagacacg cctgttgggc gacgtagtgc aggtgttccg gttgcgatag
      661 cggcgcgggg cagcaaccgt gaggacgctc ttgtcgatgc ccttcgcttg gtcaggggcg
      721 gcgccttcgg cggggtcagt cacccgcgcg cgggcaatct gaggcatcga agactcatcg
      781 ttcgagggcg cagccgcatc agatgatgga agctcggata gcctgcgctc aaaagcgtcc
      841 tccacgaact tggcatcgac tgccgcgagg ctgttcttgg cggtgagcgc ctcacccgcc
      901 caagccgcgg cgagatcagc cgacgtgatg ttgcccacct ccgctagcag tttttcgcgc
      961 agcgcgggga tcgagtctga gcgggatgtc gcctttcttg ctaccacggc catgtccatt
     1021 gccaggcgtg ttcttcgact tgaacgagcg gtcaaccgcc gacggcaaca atgagggtgg
     1081 tccatcgcag agatccgggc atcaagatcg tcctcgccgg cgatgccaac cagcgtgaac
     1141 agggcgtagc gacgggcgta ggtcagcgcg gcgcccatgc gctgtggatt ggcggtctcg
     1201 gcgatcggac agaccggcca gtccgaagca atccactcgc cagaggcgtg ggcgagcgtg
     1261 gtggtcaaat ggaccatgcc ggcggtctga tccatggcgg tcgtctgtaa ggtggcgatc
     1321 ccgtgctgcc cagcgtcttg cgcacgatgt cgagcccact ggagagcggc gcatagcgga
     1381 agctgcgctc accgtctcct ggccggccgg tgcggatggt agcggtgagc gacttttccg
     1441 gattaatgag ttcggcgtgc gccttggcca aggccgaggc cagcgccgcg accgtttcac
     1501 tggaccgggg catgggttgc ctccatctcc agcacatcaa agctcaccgc ccccgatttc
     1561 gagcgcttgg ctttcacgcc atgcccgatg gcctccctgg catcctcggg catcaaggct
     1621 ttgagctcgc ttttggcccg ctcgtggtcg agaaaggcgc tccgggtgtt gcggaagaga
     1681 gcagcgaact cggcccagga attcgatgag ctcatatcga cgatgcggac ggcctcgatg
     1741 cgcggccgcg gcggctcgcc gttgatcagg tggggcgttt cgccggattg gacgcagcgc
     1801 cagaacttct tttctgccga gaccagaacg ctcagataca gcgggtccat ggggatcgtg
     1861 atctcgaccc atttgccgct gccggtgatg attgacagca ctgaggtcct caagtgggtg
     1921 acccacatgt tatgctggat ctgagccatg tacttttcgg cagcggcctc ctccgagaac
     1981 gaccacggca gcatgaattt ggcctcaaac acggcctcaa tctcctccac ggagccgtcg
     2041 agggtggctg ccatccagga aatggccgaa tgcttgactt ggtgctggat gttggaaacg
     2101 cgccggccgg tattgcgctc gtaccagaag cgattgagct gctcggtggc ggccccgagt
     2161 tggacaacaa ggttgccaga caggtcctct cgctcggcct cgccccgttt ctcctgccag
     2221 agacacgcgc cctccttcga tggagtgccg cagaacttgc ccgcgaagcg gcgttaggac
     2281 tcgctacgat caagcgggca gaacttgctg acggcgaaac atcaatgacc atggccaacg
     2341 acttggccgt tcgccgcgct tttgaagctg ccggcgtcga attcattgag gagaatggcg
     2401 gcgggcctgg ggtgcgcctg cgcaaggcgc atcaacacgg aaaacgcaaa taaactgtta
     2461 ctacgattcg cagcttcctt ttaacgagca cgcccacgtc ggcgggtgtc gacactgagc
     2521 ggctcttgaa ggcggcggtt ttctgacgac cttccccagc cagtatactg ccaccggcga
     2581 tggcagaggg atcaaaggtg gatgtgaacg catccccggc tgtaacaacg aacgctagcg
     2641 cgaaacgtgt gcttggctcg cctgcagcta ctcacgtcca tagatgtcgt cgaggcgcac
     2701 gatgtcgtcc tccccgagat agctgccgac ctgcacctcg atcaattcga gcgggatctt
     2761 gcccggattc gcgagccgat gaatgctgcc gatcgggata tagatggatt cgttttcgtg
     2821 cacgctgcgc acgtgattgc cgagcgtgac ctccgccgtt cccttgacca cgacccaatg
     2881 ctcggagcga tggaagtgct tctgcagcga gagcttgctt cccggcttca cgacgatacg
     2941 cttcacccgg tagcgcgggg caatatccac atcctgatag tacccccatg gacgatagac
     3001 gcggcggtgc tcgaccgcgg cgcgatggtt gtgcgccttg agctgctcga ccagcgtctt
     3061 cacttgctcg gccttcgcgc gcgcgctgac cagcacggcg tccgcagtcg acaccacgat
     3121 gacgtcgtcg agcccgacca cggtggtcag cacaggttcc tccgagcgca cgaggctgtt
     3181 gcggctgtcc agcatcacga cggaaccgtc ggtggcattg ccggcggcgt cgtgatcgag
     3241 cacgtcccac accgtgctcc agctgccgat gtccgaccag cccatattgg tggtagggac
     3301 cacggcggcg agcttggtcc gctgcatgac ggcgtaatcg atcgattttt tgggcgcgcg
     3361 tgcgaacggt tcgcttgcca gccgcagaaa atcgaggtcg cgcgtgaggc cagcaaccgc
     3421 cgccttggcg gcctcggcca tcgccggctc gaagcactcg atctcgccga gcatggtcgc
     3481 ggcgtgaaac aggaagttgc cactgttcca caaatactgt tccgccacgt aattggcggc
     3541 ggtcgcggca tcgggcttct ccacgaacgc ctcgaccgtg cgtgcggatg cgccattgag
     3601 cttctccccg gggcggatgt agccgtaact cgtcgctgga tgggtgggct cgatgccgaa
     3661 ggtgacgatc cgcccctcag cggcgacggc ggcggcgcgt tggcaagctt ggcggaattc
     3721 gtcgggcttg cgcacaacgt gatcggccgc aagcacgagc acgagggcgt cgcgatcgcg
     3781 ttgggccgcg agcaaggcgg acaccgcgac cgcgggaccg gaatcgcggc ccatgggctc
     3841 gagcacgatg tcggcctcga aaccgcgccc gcgcaagtgc tccgccacga caaaccgaaa
     3901 gtcggtgttg gtgatgacga tcggacgccc gaacaagtcg ggttcggaaa tgcggccaag
     3961 tacctgctgg aatgtggacc cctcgccgac gagcgagacg aactgcttgg gcatgctttc
     4021 gcgcgagacg ggccatagcc gcctgcccgc gccgccgcac atgatcaccg ggatgatttg
     4081 agccaccatc agcaagctcc ctcgacagct acaacagcta caacagctac aacagctaca
     4141 acaactacaa caactacaac agctacaaca gctacaacag ctacaacagc tacaacagct
     4201 acaacagcga agcggtcctg gtccaaaagg tcaagctgtg ccgcgggtga attcggcgct
     4261 gccctgaaca tcagcattgg gggtacagcg ccgcgtcgtg ccagatggac gccacatcgt
     4321 cattcgcgat ccttcagcag tcagccgcgc ttagcctgga agatacggtt gagtgggtgt
     4381 gcgctccata gcaattgaat acggcctggc agccgagttg gacacatccg cggtatcaca
     4441 aatgagaaac ccagccatca ccaaattgga tcaattcgca tgcgctcgaa actgggctta
     4501 cgatctcgca tctacgatgc gcattcgacc tgggcatgcc gtacgatggg ggaagatcgt
     4561 actcgggctc ttgctggtga tgacgatcgc ttcctggctg cactccgttc cggctcagcc
     4621 cagggcgttt cagtgctcgc agctgctatg ccaccaaact gaacccgacg acggcaaagt
     4681 gatgatcgaa cgcatatgcg agccgaattc gcgcgcgctt catgatcgca aagctggtgg
     4741 cgtcgactgc ggacagcttg tgcagatccg cgcgtcgaaa atattcccaa gactgttcca
     4801 ggtcgcgcaa ctcgcatgga agaatcctta ccgtgccggg cttgtagata gcttccttcc
     4861 atgtcagcgc gacatcccga ctggcgttac gatcgagaaa tgtgaatgtc tcgatgacta
     4921 cgggaacgga actgtgaagt ttcgcgccca ctccgcgcaa cagctcccac tgctctcgcg
     4981 cttgcggatg tagtgggtcc cgtgacagcg ctagcgcaat ccacgccccg ctgtcgacga
     5041 aaaccgcttc cccttgccgc atcagcgccc gtcgtgaacg ctatcgtgtt cgatggacgt
     5101 acgcttcggc tcgccgtccc agatggcaac cgggcctgcg gcctggggct tgagcaaggt
     5161 ctttcggatc gcaccgcgga tgagctccgc gacactgcgc cccgagcgcg ccgcggccat
     5221 gcgcagcgcg tcgagttctt ccttgcggag gtaaacctgg gtctttacca tgtatgtcac
     5281 tttactggag ctgttgacac acgtcaactc gacaggctgg tagcgggcgg gtatgccgat
     5341 ctcgcttgct ccaaagggca aacccggcgc gggtcatcat cggatgctta ctttcctacg
     5401 atttggaccg agaaagcaga ctgtttgagc gggccatagc atgacggtcc tggtcaccgg
     5461 cggggccggc tatatcggaa gccacatggt tcacgagctc gtcgatgcag gcgagcgcgt
     5521 cgtggtgctc gacaacctcg cgaccggtac gattgggcag tagcgcaggg cgcgtcgctc
     5581 gtcatcggtg acgtcggcga tcaactgcgc gtagccgcgt tgatcgcgga gcatcgggtt
     5641 gacgctgtta tccatttcgc cgcctcgatc gtcgtcccgg actcggttcg cgatccgctc
     5701 ggctattacc gcaacaatac ggtgaactcg tgggccctga tcgagagcgc cgtgaagggg
     5761 ggcgtgcgcc atttcatctt ctcttcgagc gccacggtct acggccatcc cgagcgcgtg
     5821 ccggtgcgcg aagacgatgc cacggtgccg acgtcacctt atggctcttc gaagctgatg
     5881 accgagatca tgctacgcga cgccagcagc gcccacggcc tgcgccacgc gatcttgcgt
     5941 tacttcaacg tcgccggcgc cgacccgctt ggtcgcgttg gccagtcgac caaagcggcc
     6001 acccacctca tcaaggtctc gctcgaaacc gcgctcggcc tacggccaaa agtggacgtc
     6061 ttcggcaccg actacccgac accagacggc acctgcatcc gagattacat acacgtgggc
     6121 gatctcgtgc gcgcgcatag cgatgcgctg gcatatttgc gcgctggtgg ggagtctgtc
     6181 acgctcaatt gcggttacgg ccgtggtttc tccgtgctcg aagtgatcga gaccgtaaag
     6241 cgggtctccg gcgtcgactt caaggtcgcg ttcgccggcc ggcgggccgg ggacaccgca
     6301 cagatcgtgg cggcctccga ccgggctcgt tctttgctca aatggcagcc gcgtttcgac
     6361 gacctgcaaa caatcgtggg ccacgcgctg gcatgggagc gcaaaatttt ggcgcgcggc
     6421 agctgatttc tccaatttac ggctcgagca agacccggct tgcgaatttg cgcgcgagcg
     6481 ctgccgttca tagcggttcg tagtcgctaa cgggcggctt acatttgcgc cccaccgacc
     6541 gaacaagcag gctgcaattc ccctaaaagc gatactccgc tggtatgaac ttggcgatgg
     6601 tcgacgctgg tacgaaatat ttggcgatat ctttgggctc gctttcggcg tcggcgcggg
     6661 tgaaaatttt ccggctcatg atgccgagca agca
//
LOCUS       DATLJT010000395         6061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2090405,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000395 DATLJT010000000
VERSION     DATLJT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090405"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VM910_09075"
     CDS             <1..658
                     /locus_tag="VM910_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVI62721.1"
                     /translation="VKEPTDSNRAPPTCLWSKEFYDTDLSRGFVRGYGIQFGRGAGPV
                     FEAVASEQKGILPWGADHHRVFRKLNGHRLHVSAICEDLPEEHNRVTLDPVLKDSHGI
                     PAPKIDYTISENSRKMMEHALARGREILDTAGATDICINSPIPWGGWHLLGTARMGAD
                     PERSVVNEWGRSHDVKNLFIVDGSVFVTSGGVNPTSTIQAIALYVADQMKQRLANLFD
                     "
     gene            666..1112
                     /locus_tag="VM910_09080"
     CDS             666..1112
                     /locus_tag="VM910_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62722.1"
                     /translation="MSAETLTAAQRDDLRTIAAMIIPESAEYRVPGADDTRIQADILA
                     TLGRDTKQVAAALDHLARLAGMPLARLDPTLREAVAKEFRANGGAPAATLVRVVLQCY
                     YRDDRVLRSLGLELRPPFPKGHVLPDGDWSLLDPVKKRPSKLRQAP"
     gene            1279..1755
                     /locus_tag="VM910_09085"
     CDS             1279..1755
                     /locus_tag="VM910_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI62723.1"
                     /translation="MAASSPDLKEFLLATPFFGGLSDASVDLLVSMLVERRFDAGATV
                     VTEGEPGRSMFVVHSGELVVSKSGDSGRAIRMSSLGPGDFFGEMTLIEMQNRSATVVA
                     ESPTVLYELTAQKLYKCYKADVYGYVIVLQNINRELCRRLRRADNRFAELQMHASE"
     gene            1912..2940
                     /locus_tag="VM910_09090"
     CDS             1912..2940
                     /locus_tag="VM910_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVI62724.1"
                     /translation="MLDFASTRFDGVPSPQAAEQAPVNDQALLDAYSNAVIGVTDRVG
                     PAVVRVETGPKARNPRERGGLGSGIVISPDGLVLTNSHVVGSAREIRLRDIEGFITDA
                     HVLGVDPDTDLALLRADGARDLRYAALGNSKTLRRGQLVVAIGNPLGFESTVTAGVVS
                     ALGRSIRSVSGRTIEDVIQTDAALNPGNSGGPLVSSSAEVIGINTAIINGAQGICFAV
                     ASNTAQFVLSEIIRHGYVRRAYIGVSGQTAPIPRRHAVVAGVENKMGALLIQVEPDGP
                     AAQAGLLPGDVVVKLDGVEINGVDDLIRVLDRDRIGKKLAMEVLRLGRLRAIDIHPVE
                     RKPAARAG"
     gene            3182..3700
                     /locus_tag="VM910_09095"
     CDS             3182..3700
                     /locus_tag="VM910_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62725.1"
                     /translation="MPVIVLVASTLVFWVIYWFVRMGGVAHFHEVSARRKDEARRAKA
                     RESERTAPLRAIDDPRDAATVLMLLMPRGRDPTAGQIATIEERMRVVFGFDRELNERL
                     THARFIASCAQNFEEAAKLFADLFNKRLTAEERRELVGLVEEIARIDGLSAIQTADID
                     VLKRAVGLAPVG"
     gene            complement(4514..>6061)
                     /locus_tag="VM910_09100"
     CDS             complement(4514..>6061)
                     /locus_tag="VM910_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HVI62726.1"
                     /translation="CSQTSNCVSVQPLDTEFGRKRQIDQSNCNKDYSCVEGFCPSFVT
                     VHGGALKRIKTTAVESGQLFADLPLPTPPKLDTAYNILVTGIGGTGVITVGALLGMAA
                     HVDGRGCSALDFTGLSQKNGAVMSHVRIAPTPEDITTVRITNGGADLILGCDIVVSAS
                     ETALTRIDRGVTKAIVNADLQPTASFVQNPDIDFGMNAMQTTLRDAIGERNLDIIDAT
                     GLAATLMGDSIATNAFMLGYAFQKGTIPLSLEAMLRAIEINGAAVEMNKQAFTWGRLA
                     AHDMARVRSVTQFRARAGAPTRTLDEQIATRAEFLTAYQDKAYAERYLAAVAKVRKAE
                     AAAAPASTELTEAVAKNLFKLMAYKDEYEVARLYTDGSFAGKLSDRFEGDYRLKFYLA
                     PPIFAKRDKSGRLQKKEYGGWMIHAFRLLAQLKFLRGTSLDPFGRTEERRTERRLIDD
                     YLAMIEQRIPALAPAQIPLLAKLARIPEMIRGYGHIKDEIIRKARAEQARLEAELENS
                     RFAAAAE"
BASE COUNT         1091 a         1902 c         1946 g         1122 t
ORIGIN      
        1 cgtgaaggag ccgaccgact ccaaccgcgc gccgccgacc tgcctctgga gcaaggaatt
       61 ttacgacacc gatctgtcgc gcggcttcgt ccgcggctac ggcatccagt tcggccgggg
      121 tgcggggccg gtgttcgagg cggtcgcgag cgagcagaaa ggcattttgc cgtggggcgc
      181 cgaccatcac cgcgtcttcc gcaagctgaa cggccaccgc ttgcacgttt ccgcgatctg
      241 cgaggatttg ccggaagagc acaaccgcgt cacgctcgat cccgtgctca aggacagcca
      301 cggcatcccc gctccgaaga tcgactacac catcagcgag aactccagga agatgatgga
      361 gcatgccctg gcgcgcggcc gggaaatcct cgacacggcc ggcgcgaccg atatctgcat
      421 caattctccg attccctggg gcggctggca tctgctcggc accgcgcgga tgggagcgga
      481 ccccgaacgc tccgtggtga acgaatgggg ccgatcgcat gacgtgaaga acctcttcat
      541 cgtcgacggc agcgtgttcg tcacctcagg cggcgtcaat ccgacatcca ccatccaggc
      601 catcgcgctt tatgtcgccg accagatgaa gcagcgcctg gcaaacctgt tcgattgagg
      661 acgcgatgag cgcagaaacc ctcacggcgg cccagcgcga cgaccttcgc acgatcgcgg
      721 ccatgattat tccggaaagc gcggaatatc gcgtgcccgg cgccgacgac acccgcatcc
      781 aggcggatat tctggcaacg ctcggccgcg acaccaaaca ggtcgcggcc gcgctcgatc
      841 atctcgcccg ccttgccggc atgccgctcg ccaggctcga tccgacgctc cgagaagccg
      901 tggcgaagga atttcgtgcc aacggcggcg cgccggcggc aaccctcgtc cgcgtcgtgc
      961 tgcaatgcta ctaccgcgac gatcgcgtgc tgcgctcgct cggactggaa ttgcgcccgc
     1021 cattcccgaa agggcatgtg ctgcccgatg gcgactggtc gctgctcgat ccggtgaaga
     1081 agcgcccgtc gaagctgcgg caggcaccct gaagcgcgat gcttcaagat atgaaatcgg
     1141 ccttgtttcc aggacctcgc tgctgacagc aacggacctg gccgcattgg cggtagcttt
     1201 gggttgcacc tgttcgggca ggctgcaggc agcgccctgc ctgcttgccg ctaccgggag
     1261 cgagagtata ttacctcgat ggctgccagc tcgcccgatc tgaaagagtt cttgctcgcc
     1321 acaccttttt tcggtggcct ctcggatgcg agcgtcgatc tcctggtctc gatgctggtc
     1381 gagcgccgct tcgacgccgg tgcaaccgtc gtaacggaag gagaaccggg gcgctcgatg
     1441 ttcgtcgttc actccggcga gctcgtagtg agcaagagcg gggattcggg gcgcgcaatt
     1501 cgcatgtcga gtctcgggcc cggtgatttt tttggcgaga tgactctcat cgaaatgcag
     1561 aaccgatcgg ccacggtcgt cgcggagagc ccaaccgtgc tgtacgagct cacggcccaa
     1621 aagctttaca agtgctacaa ggccgacgtc tacgggtatg tgatcgtgct gcagaatatc
     1681 aaccgcgagc tttgtcgacg gctccgccgc gccgacaatc gctttgccga gctgcagatg
     1741 cacgcgagtg aatgacgcag atcgcgcgtc atgtgggctc cgaaagcccg tgcctgcccg
     1801 tttggggaag tggggcggcg acctagccga tcgggcaggg tgcaggccag ccatgcgcac
     1861 ttgcaccgac gggaaggcgt aaccatctga cagggccgaa ggaatttcgc catgttggat
     1921 tttgcctcca cccgcttcga tggcgtcccg tcaccgcaag ccgccgaaca agcccccgtc
     1981 aacgatcaag ccctgctcga cgcgtattcg aacgccgtga tcggcgtgac cgatcgcgtc
     2041 ggccccgccg tggtgcgggt cgaaaccgga cccaaagcac gcaacccgcg cgagcgcggc
     2101 gggcttggct ccggcatcgt catttcgccc gacggcctcg tcctgaccaa cagccacgtg
     2161 gtcggatcgg ccagggaaat ccgtctgcgc gacatcgaag gcttcatcac cgacgcgcat
     2221 gtgctcggcg tcgatcccga cactgatctt gcgctgctgc gtgccgacgg cgcgcgcgat
     2281 ctgcgctatg ccgcactcgg caactcgaag acgctccgcc gcggccagct cgtggtcgcg
     2341 atcggcaatc ctctcggatt cgaatcgacg gtcaccgccg gcgtggtgtc ggcgctcggc
     2401 cgctcgatcc gctcggtctc cgggcgcacc atcgaggacg tcatccagac cgacgccgcg
     2461 ctcaatcccg gcaattccgg cggaccgctg gtgtcgtcga gcgccgaagt gatcggcatc
     2521 aataccgcca tcatcaacgg cgcgcagggc atctgctttg cggttgcgag caacaccgcg
     2581 caattcgtgc tgtcggagat catccgccac ggctatgtcc gccgagccta tatcggcgtg
     2641 tccggccaga ccgcgccgat cccgcgccgg cacgccgtcg tggccggcgt cgagaacaag
     2701 atgggcgcgc ttctgataca ggtcgagccg gacggaccgg cggcgcaggc gggattgctg
     2761 ccgggcgacg tcgtggtcaa gctcgacggc gtcgagatca acggcgtcga cgatctgatc
     2821 cgcgtgctcg accgcgaccg catcggcaag aagcttgcga tggaggtgct gcggctcggc
     2881 cggctgcgcg cgatcgatat tcatccggtg gagcgcaagc ccgcggcgcg ggcggggtaa
     2941 gtgaaagcca acgctggacg cgcatttcgc ctctcagatg atctgccaac gcatttctgt
     3001 tgccatgaag agcgttgcgg tcagcggtac gccctatgtg agagaattgc gccgatggat
     3061 tgcagctcgc gcggaagacg ttcttaacca atcgtagctt ccaggccaaa gatgcaccac
     3121 gcctgcgtaa tcatgccgtt acactatgtc gagtagagtg gaacgtcgca ggggccttga
     3181 aatgccggtt atcgtccttg tcgccagcac gctggtgttt tgggtgatct attggttcgt
     3241 ccgcatgggc ggcgtcgcgc acttccatga ggtgtccgcg cgccgcaagg atgaagcgcg
     3301 acgtgcaaag gcgcgcgagt cggagcggac cgcgccgttg cgtgctatcg acgatccgcg
     3361 cgacgccgca acggtcctga tgttgctgat gcctcgcggc cgcgatccga cggccgggca
     3421 aatcgccacc atagaagaaa ggatgcgcgt ggtgttcggc ttcgaccgcg agctgaacga
     3481 gcgcctcact catgcacgct tcattgcaag ctgtgcgcaa aattttgaag aggcggcgaa
     3541 actgttcgcc gatctcttca acaaacggct caccgccgaa gaacggcgcg aactggtggg
     3601 cctcgtggag gagatcgcgc ggattgacgg actctccgcg atccagaccg cggacatcga
     3661 tgtcctcaag cgggcggtcg gcctcgcccc ggtcgggtga gtcaagcgct gcgcacttgc
     3721 cgcgcgccgc aggaacaaag cccttccaga acaagcagtt cttcgaagtg atttgcccga
     3781 caagtgagtt gcgcgaattc ctgtcaagtc gccccgcaag aaacatttcg ctttatcaga
     3841 accccaaatc aatgtcatga cccgcgccgt cccgcctcga tgagaggggc gcttcgcgat
     3901 cgtcacgagt gttgggcgcg ggatgcgatg gacgcgaagg catcgcccga cgtgcgatgc
     3961 attttgcgga cggcgaagtc gtgtggtcct ggcgcccctt ggctggcgtc aagtttgtcg
     4021 aggatgatcg acaggcgacg gtgactaaga agtcatggac accggggaga gcacgaagca
     4081 gcgttaacac catcgcgcag ggaatgtcga tgtttcggct tcacctgtgg tgacttacgc
     4141 ttgtgtgctc ttctcaatcg cacacaaggc tgcgggtgca gcagatcacc cggcattccc
     4201 tgcgccctct cgagtcgagg acgtggtttt gcaaaaactc ggacaagcat gtcgcgggaa
     4261 tgcgaggtcg tgtcctctcg gctgtttgaa attcgaatca gaaaatacgc ggtcgtcccg
     4321 gacaagcgag ttgcttgcgg cgcgcagcgc cgtcaagtgg cgagcgcgat ccgggaccca
     4381 taaccacggg cctttgttat acgaagatcg tccgacaccg ctgcgagcca cggagaaatt
     4441 ccgcggtatg ggtcccggcc ttcgccggga caacagcgga cggcaaaaac gctaccctgc
     4501 gatctgggca aagctattcc gcggccgctg cgaaccggct gttctcgagc tccgcttcga
     4561 gccgggcctg ctcggcgcgc gccttcctga tgatctcgtc cttgatgtga ccgtagcccc
     4621 ggatcatttc gggaatgcgc gcaagttttg cgagcaacgg gatttgcgcc ggcgcgagcg
     4681 cagggatgcg ctgctcgatc atcgcaagat agtcgtcgat cagcctgcgc tcggtgcggc
     4741 gttcttccgt gcgcccaaac ggatcaaggg acgtgccgcg caggaattta agctgcgcca
     4801 gcaggcggaa cgcgtggatc atccagccgc catattcctt cttttgcagg cggccggatt
     4861 tgtcgcgctt tgcaaagatc ggcggcgcca gatagaattt gaggcggtag tcgccctcga
     4921 acctgtcgga cagttttccg gcgaagctgc cgtcggtata gagccgcgcg acttcgtact
     4981 cgtccttgta ggccatcagc ttgaacaggt ttttcgcaac tgcctcggtg agttcggtcg
     5041 aagccggcgc agccgcagct tccgcctttc gtactttggc gaccgccgcg agatagcgct
     5101 cggcataggc cttgtcctga taagcggtca gaaattccgc gcgtgtggcg atctgctcat
     5161 ccagcgtccg ggtcggcgca ccggcgcggg cgcggaactg ggtcacgctg cggacgcgcg
     5221 ccatgtcgtg ggcggcaagc cggccccagg tgaaggcctg cttgttcatc tcgaccgcgg
     5281 cgccattgat ctcgatcgcg cgcagcatcg cctcgagcga cagcgggatc gtgcccttct
     5341 ggaaggcgta gcccagcatg aaggcgttgg tcgcgatgct gtcgcccatc agggttgcgg
     5401 ccagcccggt cgcatcgatg atgtcgagat tgcgctcgcc gatggcatcg cgcagcgtcg
     5461 tctgcatcgc attcatcccg aaatcgatgt cgggattttg cacgaagctt gccgtcggct
     5521 gcagatcggc gttgacgatg gcctttgtca cgccgcgatc gatgcgcgtg agcgccgttt
     5581 cgctcgccga caccacgatg tcgcagccca ggatcaggtc ggcgccgccg ttggtgatgc
     5641 gcacggtcgt gatgtcttcc ggcgtcggcg cgattcggac atggctcatg accgcgccgt
     5701 tcttttgcga caggccggtg aagtcgagcg ccgagcagcc gcggccatcg acatgggcgg
     5761 ccatgccgag cagcgcgccg acggtgatca cgccggtgcc gccgatgccg gtgacgagga
     5821 tgttgtaggc cgtgtccagc ttcgggggtg tcggcaacgg caagtcggca aagagctggc
     5881 cggactcgac cgccgtggtc ttgatgcgct tgagcgcacc gccgtgaacg gtgacaaagc
     5941 tcgggcaaaa gccttcgacg caggaataat ccttgttgca gttcgactgg tcgatctggc
     6001 gtttgcggcc gaactcggtg tcgagcggct gcaccgagac gcagttcgag gtctgcgaac
     6061 a
//
LOCUS       DATLJT010000396         1556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2550297,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000396 DATLJT010000000
VERSION     DATLJT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550297"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VM910_09105"
     CDS             complement(<1..135)
                     /locus_tag="VM910_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVI62727.1"
                     /translation="MNWDRVEGNWKEFKGKVQQKWGKLTNDDLDVIEGKRTQLSGRLQ
                     Q"
     gene            669..989
                     /locus_tag="VM910_09110"
     CDS             669..989
                     /locus_tag="VM910_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62728.1"
                     /translation="MRRYSGYFVGCGGAVLAIATFLVGLEVSSTTAAPSIARTAPMVN
                     RTLKSDRMPLASTKSRNAVNGPAETIVPPAPAPKPRLLDGCEPVVSAIGHSPLSQVPG
                     RCVS"
     gene            1341..>1556
                     /locus_tag="VM910_09115"
     CDS             1341..>1556
                     /locus_tag="VM910_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62729.1"
                     /translation="MPEAQDKGERADTPVSERAAPSHSDLESFRSALDAGRIGLWSWD
                     LRSHQLAWSTKLEDFHGRREETLEGTFS"
BASE COUNT          270 a          507 c          476 g          303 t
ORIGIN      
        1 ctgctgaagc cggccgctga gctgcgtgcg cttgccctcg atgacgtcaa gatcatcgtt
       61 ggtgagcttg ccccacttct gctgaacctt gcctttgaac tctttccaat tgccttcaac
      121 tcgatcccaa ttcatcgcgt ctctcctgca ttgttttccc ggtgttgtgc ttcgttcgct
      181 cggcgggaat gtccagccgg aaaaccctac gcgacagcaa aggttccgct gcgctgatcc
      241 ccgtgccgct tctgaaaaag acgtgtgaaa aagacgcggg cgtagccgac ctctggggcc
      301 gactacgccc tagctaaccg gccgagcacg gggaggggga aatgggcggc cggctatccc
      361 tgacaatgca cggcgcatcg gttggttcct ggggcaatcc cgcaaaaccc cggatcaagc
      421 ctgcagggca tgaggcccgg cgagactagt ccttcggctg agttggacac cgccgcaaac
      481 ggcgcgccga ccgacgtttc tgtccgcgcc ggcacccggc cccgctccgt gcggaaacct
      541 cctttgccgc caaagcgtta gcgccaggcg agatcacgag cgcggctctg cagaaattgc
      601 agcaccgggc atgaacctat cagcgggtta ccgcgactaa tgagcacagg gatcgtgggg
      661 agtaacccat gcggcgttat tcgggatatt tcgtcggctg cggcggcgca gttttggcca
      721 tcgcgacctt cctcgttggt ctggaagtct cctccaccac cgctgcgccg tcgatcgctc
      781 ggaccgcgcc gatggtgaac cggaccctca agagcgaccg tatgccactc gcctcgacca
      841 agagccgcaa tgcggtgaac ggtccggctg aaacgatcgt cccgccggcg cccgctccca
      901 agcccagatt gctcgatggt tgcgagccgg tggtcagcgc aatcggacat tcgccattgt
      961 cgcaggtccc gggccgctgc gtgtcctagg cctaccgccg gtcccctcct ccaacatcgc
     1021 ttctttccgc tttccgccgt gttcggcgtg ctgttgcgca agcgccgcag gcgggcgctt
     1081 agtcacgccg cggcgcggcc agtcgcctcg cgccgcggaa ccttcgaaat accagcggct
     1141 tatagaggaa gatgggtgat ggaggctgcg cgacttgctt gcggcatagt gtcggcctga
     1201 gcggacggcg attggttcgc cgcccgcgcg tttgcgcctc gagcgcacct taagggagga
     1261 cgcatcagcg atgtgctacc tttctccgcc tgggacctct gccgcggaca aaatagaacc
     1321 gtcgcacgct ggacgtgaaa gtgcctgaag cacaagataa aggtgaaagg gccgacactc
     1381 ctgttagcga acgagcggcg ccttcccatt ccgatcttga gtcgtttcgc tcggcgctcg
     1441 atgccggccg gatcgggctg tggtcatggg accttcgctc ccatcagttg gcttggtcga
     1501 ccaagcttga agacttccac ggccgccgcg aggagacgct cgagggcacg ttctcg
//
LOCUS       DATLJT010000397         3684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_252512,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000397 DATLJT010000000
VERSION     DATLJT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252512"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1046)
                     /locus_tag="VM910_09120"
     CDS             complement(69..1046)
                     /locus_tag="VM910_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HVI62730.1"
                     /translation="MFSVFVPAESSLKKVTVEDLSKLPEKAVWVDLVNPTAAEDRAVE
                     RLAGIAVPTREDMQEIEISSRLYIESGARYMTATLMCQSDSDTPKTTAVTFILAGHRL
                     VTVRYELPKPFALVENKLARYCAPSITGEQVMMELLDAVIDRCADILERVGADVDQVS
                     HEIFEPESERHGSAKRYAQILITIGRKGDLTSKVRESLVSIGRLVTFLSAAIEGVKWT
                     KDMREQLKTQQRDVAALTDHASYLSNKITFVLDAMLGVVNLEQNNIIKLFSVMAVVLM
                     PPTLIASIYGMNFKYMPELDWVHGYTFALIAMVLAAVLPYLAFKWKKWL"
     gene            1389..2075
                     /locus_tag="VM910_09125"
     CDS             1389..2075
                     /locus_tag="VM910_09125"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI62731.1"
                     /translation="MSSLTVKLSILAAGLMLSGCMQSATYEATNTANFKPRDREYLAK
                     IRYTNTPVAEPFRRALVEYHRKEAPGSIVVDSDNHYLYYVLNDGRAIRYGITVGEEAM
                     AWSGIAKIGAMTEWPPWHPTKSEIERLGVPTFVAPGPDNPMGSRAMYLYANGKDTLFR
                     IHGTNQPEYIGASISSGCIRLTNEDAIDLYNRVKVGTIVVVLDPHHGDSPFNSQMALQ
                     GGSQGFPTLQ"
     gene            complement(2140..3066)
                     /locus_tag="VM910_09130"
     CDS             complement(2140..3066)
                     /locus_tag="VM910_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HVI62732.1"
                     /translation="MVTIAVALNVAWAHPGPAAAAPKRGGSDDLFGETPPKLRGTLHV
                     TRVPLPKPRPAEAPGRTPAGAAIEKPEPDKTTPAEAEKQVAPEPPSPSACRRALTDDI
                     AIAPSIPDLHGPGGCGGTDLVRLEAVVLPNKHKVAVTPAATLRCAMASAIADWVRTEV
                     TALAARQNSEPAVLDNFDSYECRGFNRVPGAHLSEHGRANALDVRAIKLANGQSIELT
                     DRSVPREVRESVLHAVCTRFTTVLGPGSDGYHENHIHLDLAERRNNYRICQWNVWDSL
                     PQVAPLLPAERPEDAPPREEAKDGVKSDAKTR"
     gene            3214..>3684
                     /locus_tag="VM910_09135"
     CDS             3214..>3684
                     /locus_tag="VM910_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI62733.1"
                     /translation="MLGLMQDWPLLCHKIIEHAARYHGNQEIVTRSIEGPIVRTNYRQ
                     VHDRALKVSQMLEREGIKFGDRVATIAWNTARHLECWYGIMGIGAICHTVNPRLFPEQ
                     IAWIINHAQDRIVMTDITFVPILEKIADRMPSVERYVVLTDKAHMPETTLKNAVN"
BASE COUNT          677 a         1182 c         1149 g          676 t
ORIGIN      
        1 tttcctgcga ttgccctgcc cgcaggcggg gaaagggagc gggagagttt cgccgataga
       61 ataagcgcct acagccactt cttccatttg aaggcaagat agggcagcac ggccgcgagc
      121 accatcgcga tcagcgcgaa ggtgtagccg tgcacccagt cgagctcagg catgtatttg
      181 aaattcatgc cgtagatcga ggcgatcagc gtgggcggca tcaggaccac cgccatcacg
      241 gagaacagct tgatgatgtt gttctgctcg agattgacca cgcccagcat ggcgtcgagc
      301 acgaaggtga tcttgttgga gaggtagctt gcgtggtcgg tgagagcggc gacgtcgcgc
      361 tgctgggtct tgagctgctc gcgcatgtcc ttggtccatt tcacgccctc gatcgccgcc
      421 gacaggaacg tgacgaggcg gccgatcgag acgaggcttt cgcggacttt cgaggtgagg
      481 tcgcccttgc ggccgatggt gatcaggatc tgagcatagc gcttggcgct gccgtggcgc
      541 tcgctctccg gctcgaagat ctcatgggag acctggtcga cgtcggcgcc gacgcgctcc
      601 agaatgtccg cgcagcggtc gatcacggca tcgagcagct ccatcatcac ctgctcgccg
      661 gtgatgctcg gcgcgcagta gcgcgcgagc ttgttctcca ccagcgcaaa cggcttgggc
      721 agctcgtagc gcacggtcac caggcggtga ccggcgagga tgaaggtcac ggcggtcgtc
      781 ttcggcgtgt cgctgtcgga ctggcacatc aaggtcgcag tcatgtaacg cgcgccgctc
      841 tcgatataga ggcggctgga aatctcgatc tcctgcatgt cctcccgcgt cggcaccgca
      901 atcccggcga gccgctccac cgcacggtcc tccgcagccg tcgggttgac gaggtcgacc
      961 cagaccgctt tctcaggcag cttcgaaagg tcctcgaccg tgaccttctt cagcgaggat
     1021 tctgcgggaa caaacaccga aaacatgcaa aactccgcaa aggcccacgg ggacctaacc
     1081 cgacctcacc cgattttggc aagttgccct taaccgcccg ttgaaatttg tggcgtcgag
     1141 gcggccgctt cgtggcaagg accaggcgcg ccccataatc ctgcccacaa atcggacgct
     1201 ccgtaccttc tttgcggcaa aaaagccaca gccaggctcc cgcaacgcga gacgagtctt
     1261 ccagctctgg aattatcgct gatatcgacg ataatggatt taacggatta acggaatcgt
     1321 ggcgccttgg ggcgcagcct cacgagcgat tcaggttgcg gtttttactt gcgattggaa
     1381 gttcggtcat gtcgtcgctt acggtgaaac tctcgatttt ggccgccgga ctgatgttgt
     1441 ccggctgcat gcagtccgcg acctatgagg ccaccaacac ggccaacttc aagccacgcg
     1501 acagggaata tctggcgaag atccgctaca ccaacacgcc ggtcgcggaa cccttccgtc
     1561 gcgcccttgt cgaatatcac cgcaaggaag cgcccggctc gatcgtggtc gattccgaca
     1621 accactatct ctattacgtg ctgaatgacg gccgcgccat ccgttacggc atcaccgtcg
     1681 gcgaggaggc catggcctgg tccggcatcg ccaagatcgg cgcgatgacc gagtggccgc
     1741 cctggcatcc gaccaagagc gagatcgaac ggctcggcgt gccgaccttc gtcgcacccg
     1801 gtccggacaa tccgatgggc tcgcgcgcga tgtatctcta tgccaacggc aaggatacgc
     1861 tgttccgcat ccacggcacc aaccagccgg aatatatcgg agcgtcgatt tcctcgggct
     1921 gcatccgctt gaccaacgag gacgcgatcg acctctataa ccgcgtcaag gtcggcacca
     1981 tcgtggtggt gctggacccc caccacggcg actcgccgtt caactcgcag atggcgctcc
     2041 aggggggcag ccaaggtttc cctacgctgc agtgaggcag gggcctcacc cgtgaatgca
     2101 aaagcgccgg gccaccggcg ctttttcttt ggcgaagcct cacctcgtct ttgcgtcgct
     2161 cttcacaccg tccttggcct cctcgcgcgg cggcgcatcc tccggccgct cggccggcaa
     2221 cagcggcgcc acttgcggca gcgagtccca cacgttccac tggcagatcc ggtaattgtt
     2281 gcgccgctcg gcgagatcga gatggatgtg gttctcgtgg tagccgtccg agcccggacc
     2341 gagcacggtg gtaaagcgcg tgcacactgc atgcagcacg ctctcgcgca cctcgcgcgg
     2401 cacgctgcgg tcggtcaatt cgatcgactg gccgttggcg agcttgatcg cgcgcacgtc
     2461 gagcgcattg gcgcggccgt gctcggacag atgcgcgccg ggaacgcggt tgaagccgcg
     2521 gcattcgtag gagtcgaaat tgtcgagcac ggcgggctcg ctgttctgcc tggccgcgag
     2581 cgccgtcacc tcggtgcgaa cccagtcggc gatcgctgac gccatcgcgc agcgcaaggt
     2641 cgccgccggc gtgaccgcga ccttgtgctt gttgggcagg accacggcct caagccgcac
     2701 cagatccgtt cccccgcagc cgccgggccc atgcaggtcg gggatcgacg gcgcaatcgc
     2761 gatatcgtcc gtgagcgccc gccggcacgc cgagggcgat ggcggctccg gcgccacctg
     2821 cttctcggcc tccgcggggg tcgtcttgtc gggctccggt ttctcaatcg cggccccggc
     2881 cggcgtccgc cctggcgcct ccgcgggccg cggttttggc agcggcacgc gcgtgacatg
     2941 cagggtgccg cgcagtttcg gcggcgtctc gccgaacaga tcgtcagaac ccccgcgctt
     3001 cggcgcggcc gcggcgggcc ctggatgtgc ccatgcgaca ttcagcgcga cggcaatggt
     3061 aaccattgcc gcgccgccgt gacggctgct gccaaaagcc catttgcgag gtccgcccgc
     3121 cgcgctaaag ttcatctcaa ttccgtgatg gaacgccgtt ccgcccgtcc aaggcggtca
     3181 aggcgcaagc aataacattt gaggagggaa attatgcttg gtttgatgca ggattggcct
     3241 ttgctgtgcc acaagatcat cgagcacgcg gccaggtatc atggcaacca ggagatcgtg
     3301 acgcgttcga tcgaagggcc gatcgttcgc accaattaca ggcaggtcca tgatcgcgcg
     3361 ttaaaagtct cgcagatgct ggaacgcgag ggcatcaagt tcggcgaccg cgttgccacg
     3421 attgcctgga acaccgcgcg gcacctggaa tgctggtacg gcatcatggg gatcggagcg
     3481 atctgccata ccgtcaatcc gcggctgttc ccggagcaga tcgcctggat catcaaccat
     3541 gcgcaggacc gcatcgtgat gaccgacatc accttcgtgc cgatcctgga gaagatcgcc
     3601 gacaggatgc cgagcgtcga gcgctatgtg gtgctgaccg acaaggcgca catgcccgag
     3661 accacgttga agaacgcggt caat
//
LOCUS       DATLJT010000398         5480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_941697,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000398 DATLJT010000000
VERSION     DATLJT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941697"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..585)
                     /locus_tag="VM910_09140"
     CDS             complement(211..585)
                     /locus_tag="VM910_09140"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI62734.1"
                     /translation="MRSPDVRFDCAKILGKFWHQHEPGKHMPAYVIGEHIITDPAKFG
                     EYVTKAKPMIAKHGGRYLTKGSTHKLPEGGHWKPERVVIIEFPDMDALDAWYNSPECQ
                     PLIALRKSCTSDQDMTFALEGA"
     gene            complement(666..1145)
                     /locus_tag="VM910_09145"
     CDS             complement(666..1145)
                     /locus_tag="VM910_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVI62735.1"
                     /translation="MADKRHTYSIRLNWTGNQGTGTSAYRAYSRAHEISAHGKTAIAG
                     SSDPMFRGDAARWNPAELLIAALSACHQLWYLHLCADAGILVMAYSDNASGVEIEQAD
                     GAGQFESVTLRPHAELAAGSDENIARRLHDAAAEKCFIARSVSFKVKHEPTFEIASA"
     gene            complement(1247..2719)
                     /locus_tag="VM910_09150"
     CDS             complement(1247..2719)
                     /locus_tag="VM910_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVI62736.1"
                     /translation="MTWRGWADLYAWQLDRASRTPLTRQIYMQARSAVLSGALRPGTR
                     VPSSRAMASKLGVARASVVLAYEHLLAEGYVESRHGSGTFIAEDLTGLVPRRRSASRA
                     TKRAMPTPVQNFSDFERSAVQSDARPFNTGRTLIDARTAETWRSLTHRAVRRLGANDF
                     GYTDPAGLAELRANICDYLRAARAVRCDPEQIVITAGTQQAIDIAIRVLLVPGDEVWV
                     EDPGYPLTHAQLLLAKVRPHPIPVDAQGLVVDAGLRKAPRARAAFVTPSHQFPTGVAL
                     SMARRLELLAWARQSGAFIVEDDYTSEFRYSGPPLASLQGLDNTEQVIYVGTLNKALF
                     PGLRIGYAVVPRALLQAFTGARYLIDRQPATLQQTVVSEFMQQGHFAAHVRRMRQLYR
                     EQRDALAEILMQRAAGRLDVAVPDQGMHLVAYLCDASSDIAIEAAAQRAGIVVRAISR
                     FYRAARPRRGLMLGFSGFPRQLIVPSAARLAALIVGHGRA"
     gene            2841..4100
                     /locus_tag="VM910_09155"
     CDS             2841..4100
                     /locus_tag="VM910_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62737.1"
                     /translation="MTPSELLDLAWSYPLFEALYGRRSRRFGLGFEMPVGPFRYKSAH
                     TPVPLTEIEEALLVGAGAGFSGLALWDLPTPAPYRHRSGRTFPTTRAGGQTALFFTND
                     EGLYVLDTNFATSKLREIETVDERTKLLETYRAQRRPLRHGRLDIPRRVPPMSGHDLW
                     DSNRPGSTLFLPVCDVSASLISLIAQFVDPALRRYAPAGGGMNIVDDRHRCRPAGTER
                     WLKGGFLDAERMLPLSIVERQACYYVFSEPAAICQNMFLAAEALGLGGWKHCGFLSLE
                     ILKHIGFRIVAENGGATFGNPVGLDRVFEASCPPYHPTMDAAVDAVITPPSLSRKETV
                     PVPHRMSEAEHRAGTIQISAEGLACTKAVCNYIHDTYGRFPGGTDAMHLMWVMQAHHI
                     DTDYYDRFFGPGAYGPAHAAHMATWHR"
     gene            4114..4515
                     /locus_tag="VM910_09160"
     CDS             4114..4515
                     /locus_tag="VM910_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62738.1"
                     /translation="MIEVSSGGDQFPELRILAGHIGVPWLGEMLSLVKNDTSAYKVIR
                     YPKDLVDYMRRSNRQKVLFGSNHPFWPACECLADFDKLGLSEKAATLFLADNAKTSSS
                     STKSSARALAGSDRSQAVKEYRHAVDDRAQA"
     gene            4487..5284
                     /locus_tag="VM910_09165"
     CDS             4487..5284
                     /locus_tag="VM910_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI62739.1"
                     /translation="MPWTIERKHDHVAVVTMNTNKVNAQNPVFFADLHAAFDRLEAEF
                     SDCAVVLGGTGAVFSAGLDFDHHFPLFARRSLKEIDAWFEAYRTTNLRLFTYPRPTVA
                     AINGHAYAGGLITALGCDHRIAAEGALQFSLNEVLIGIPMPAVYCEIIKYTIGPRAAS
                     ELTLLGRVYDLAAVVKMGVIGKTAAPSRLLDDAVTWAALVPPDCYPAYAFTKRALQAT
                     TMAAIDAAARFDRDWLSRGMSDPASLRAQARRYRELKGREITWVLPS"
BASE COUNT         1057 a         1687 c         1695 g         1041 t
ORIGIN      
        1 tggagatcga ccgtatagcg ccaaacgcca taatcaccaa agttgatgtt tggcgctcgc
       61 catttcgttg cgccaatccc agcttagcaa agttcgccgc gatacgtcgc acctcatcct
      121 tttagaaagc aacttggccg cgccgagcgc cggccgggct catcctcgaa ataggtatgc
      181 gcgcttcaat cggaggtgct gtggtgaaag ctacgcgccc tcaagcgcga acgtcatgtc
      241 ttgatcgctt gtgcacgact tacgaagcgc gatgagcggc tgacactcag gcgagttata
      301 ccaggcgtcg agcgcatcca tgtcagggaa ctcgatgata acaacgcgct ccggcttcca
      361 atggccgccc tcaggcaatt tgtgggttga ccccttcgtc agatagcggc caccgtgctt
      421 ggcgatcatt ggcttggcct tagtcacgta ttctccgaat ttagctggat cggtgataat
      481 gtgttcacca attacataag ctggcatgtg cttccccggt tcatgctgat gccaaaactt
      541 gccaagtatc ttggcacaat caaagcggac atcgggtgag cgcaatggaa tgtccgctgt
      601 gtgccaagaa gagacatttg agatcacttt gcaatgatca attttcgagg ggcggctgca
      661 gcggcttacg ccgacgcaat ctcgaaggtg ggttcgtgct ttaccttgaa cgacacagag
      721 cgcgcgataa agcatttctc ggcggcggca tcgtgcaggc ggcgcgcgat gttctcatca
      781 gagccggccg cgagctcggc gtgcggccgc aatgtgacgc tttcgaattg cccggcgccg
      841 tccgcctgtt cgatctccac gccggaggca ttgtcgctgt atgccatgac caggataccg
      901 gcgtcggcgc aaaggtgcag ataccagagc tgatgacagg cagaaagcgc agcgataagc
      961 agctccgccg gattccatcg cgccgcgtcg ccgcggaaca tcgggtcgga cgaaccagct
     1021 atcgccgttt tgccatgcgc gctgatctcg tgcgcacgcg aataggcgcg gtacgccgac
     1081 gtgccggtgc cctgattgcc ggtccagttc agacgaatcg aataggtgtg acgcttgtct
     1141 gccacggatt actccggcct gcggcaattg gttatcgcag aaggttaagc tatcggggtc
     1201 ccattcgtcg atctgatgtt tcgtctgtgg cttcggccat gtagcattaa gctcggccgt
     1261 gcccaacgat gagcgccgca agtcgcgccg ccgacggtac gatcaactgc cgcggaaaac
     1321 cgctgaaccc tagcatgagg cctcggcggg ggcgcgctgc acgataaaag cggctaattg
     1381 cgcgcacgac gatgccggcc cgttgggccg cagcctcgat cgcgatgtcg gacgatgcat
     1441 cgcacaagta ggcgacgagg tgcatgccct gatcgggcac cgcaacgtcg agccgacccg
     1501 ccgcgcgctg catcagtatc tccgccaacg cgtcgcgctg ttcgcgatat aactggcgca
     1561 tgcgacgaac gtgcgcagca aaatggcctt gctgcatgaa ctcggaaaca accgtctgct
     1621 gcagcgtcgc aggttggcga tcgatgagat agcgcgcgcc tgtgaaggcc tgcagcaacg
     1681 cacgcggcac caccgcgtag ccgatgcgaa gacccgggaa tagcgccttg ttgagcgtgc
     1741 cgacgtaaat cacctgttcg gtgttatcga ggccctgcag cgatgcgagg ggcggccccg
     1801 aatagcggaa ctcactggtg tagtcgtcct cgacgatgaa cgcgccgctc tgccgcgccc
     1861 acgccaacag ttccaggcgt cgcgccatgg atagggcaac gccggtcggg aattgatgcg
     1921 agggtgtgac gaacgctgcg cgcgctcgcg gtgccttgcg cagaccggcg tcaacgacca
     1981 gaccttgggc atcgacggga ataggatgcg gccgcacctt ggcgagtagc aactgcgcgt
     2041 gggtcaacgg ataaccggga tcctcgaccc acacctcgtc gccgggaacg agcagcacgc
     2101 ggatcgcgat gtcgattgcc tgctgggtgc cggccgtgat gacgatctgc tcggggtcgc
     2161 aacgcactgc gcgcgccgcc cgtaaatagt cacagatgtt cgcgcgcagt tcggccaggc
     2221 cggcgggatc ggtgtagccg aagtcattcg cgccgagccg acgcacggcg cgatgcgtca
     2281 ggctgcgcca cgtctcggcg gtacgcgcat cgatcagcgt acggccggtg ttgaacgggc
     2341 gggcatcgct ctgcaccgct gatcgctcga agtcggaaaa attctgcacc ggcgtcggca
     2401 tggcgcgctt tgtcgcccgc gaagcgctgc gccgtcgtgg aacaaggccg gtcagatctt
     2461 ccgcgatgaa tgtaccggag ccgtggcggc tctcgacata gccctcggcc agcaggtgct
     2521 catacgccaa aaccacggac gcacgcgcca cgccgagctt cgatgccatg gcacgggagg
     2581 atggtacccg cgtgcccgga cggagtgcgc ccgacagcac ggcggagcgc gcctgcatat
     2641 agatctgccg tgtaagcggc gtgcggctcg ctcgatcgag ctgccaagcg tagagatcgg
     2701 cccatccgcg ccatgtcatc ggaaaaatcg ctacaagtgg tctgctgcaa tgagcaaaag
     2761 tggaccatcc tgccgatcca gtaaagggct aggttctgca cgttccccgc gcgtcgcggc
     2821 tttctgcaag gatcgagcta atgacaccgt ctgaactgct ggacctggcc tggagctacc
     2881 cattgttcga ggcgctctat ggccgccgct cgcggcggtt cgggctcggc ttcgagatgc
     2941 cggtggggcc cttccgctac aagtccgcgc atacaccggt gcccctgacc gagatcgagg
     3001 aagcgctgct agtcggcgcg ggcgccggat tctcgggact ggcgctctgg gatctgccga
     3061 cgccggcgcc gtaccgtcac cgcagcggcc gcacattccc gacgacgcgg gctggcggcc
     3121 agacggcgct tttcttcacc aacgatgaag ggctctatgt cctcgatacc aacttcgcga
     3181 caagcaagct gcgcgagatc gagactgtgg acgagcgcac caagctcctc gaaacatacc
     3241 gggcgcagcg tcggccgcta cgccatggcc ggctcgacat cccgcgccgc gtaccgccga
     3301 tgagcggtca cgacctctgg gactcgaacc ggccgggctc gaccctgttt ctgccggtat
     3361 gcgatgtaag cgcttcgctg atttcattga tcgcgcagtt cgtcgatcct gcgctgcgcc
     3421 gctacgcgcc tgcaggcggc ggcatgaaca ttgtcgatga ccgccaccgc tgtcggccag
     3481 ccggcacgga gcgctggctg aagggcggct tccttgatgc cgaacggatg ctgccgctgt
     3541 cgattgtcga gcggcaggcc tgctactacg tctttagcga accggcggcg atctgccaga
     3601 acatgttcct ggcagccgag gctctggggc tgggcggatg gaagcactgc ggatttctct
     3661 cgctcgaaat tcttaagcac ataggcttcc gtatcgtcgc agagaatggc ggagcgacat
     3721 tcggtaatcc cgtcgggctc gaccgcgtgt tcgaggcaag ctgtccgccc tatcacccga
     3781 ccatggatgc agcagttgac gcggtgatta caccgccgtc acttagccgt aaggaaaccg
     3841 tgccggtgcc gcatcgcatg tcggaggcgg agcatcgcgc tggcacgatc caaatcagcg
     3901 cggaggggct cgcctgcacc aaggcggtct gcaactacat ccacgacact tacggtcgct
     3961 ttccgggcgg caccgatgcg atgcatctga tgtgggtgat gcaggcacat cacatcgaca
     4021 ccgactacta cgaccggttc ttcggtcccg gcgcctacgg gccggcgcac gcggcgcaca
     4081 tggcgacttg gcaccggtga cgtcgacgcc gctatgatcg aagtatcatc tggtggagat
     4141 cagtttcccg aattgcgtat ccttgcgggc catatcggcg ttccctggct cggcgaaatg
     4201 ctgtcactcg tgaagaacga cacatcggct tacaaggtca tcagatatcc aaaagacctt
     4261 gtcgactaca tgcgtagaag caaccgccaa aaggtgctct tcggttcgaa ccatccattc
     4321 tggcccgcct gcgaatgcct tgccgatttc gacaaattgg ggctcagtga aaaggccgcg
     4381 acgttgttcc tcgcggacaa tgccaaaaca tcttcaagct caactaagag ctcagctcgg
     4441 gctctcgcag gaagcgaccg ctcacaagcc gtgaaggagt atcgacatgc cgtggacgat
     4501 cgagcgcaag catgaccacg tcgccgtcgt gacgatgaac acgaacaagg tcaacgcgca
     4561 gaaccccgtc tttttcgcgg accttcatgc cgcattcgat cggctcgaag ctgagttcag
     4621 cgactgtgcg gtggttctcg gcggcaccgg cgccgtgttc tcggcgggtc tcgacttcga
     4681 ccaccacttt ccgttgttcg cgcgccggtc gctgaaagaa atcgacgcgt ggttcgaggc
     4741 ttaccgcacc accaatctgc ggctcttcac ctatccgcgg ccgaccgtgg cggcaatcaa
     4801 tggccatgcc tatgccggcg ggttgattac cgcgctcggc tgcgatcatc gcatcgccgc
     4861 agaaggcgct ctccagttct cgttgaatga ggtactgatc ggcatcccga tgccggcggt
     4921 ctactgcgag atcatcaagt acacgatcgg cccgcgtgcg gcgagtgagt tgaccctgtt
     4981 gggtcgagta tatgatctcg cggcggtcgt gaagatgggc gtaattggca agactgctgc
     5041 gccaagccga ctactggatg acgcggtgac ctgggctgct ctcgtgccgc ccgactgtta
     5101 cccggcctac gcgttcacga agcgtgcctt gcaggcgaca acgatggccg caatcgacgc
     5161 cgcggcgcgg ttcgatcggg attggctgtc gcgtggcatg tcagatcctg cgagcctacg
     5221 cgcgcaggcg cgccgctacc gcgagctgaa gggccgagag atcacttggg tactacccag
     5281 ctaagaccac ctgtcatgtc tgggtcgggt caaaagcggg cattcggcaa tgtcgcgccg
     5341 atgtccgctt tacccccaaa agcggacatg cgagctactt cggctctacg ttggcttgtt
     5401 gctcaacaac aaccggaagc gtgaacgcaa atgtggagcc ttggctcagc aacgctgtgg
     5461 cgactcgcgt caagttcgaa
//
LOCUS       DATLJT010000399        10303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3928517,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000399 DATLJT010000000
VERSION     DATLJT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928517"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VM910_09170"
     CDS             <1..807
                     /locus_tag="VM910_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI62740.1"
                     /translation="AIADFSEAIRLDPKFAAAYSDRATAYLLQGNSDRAIADFSAAIR
                     LDPKLASAYNDRGKVYQLQGNNDRAIADLSEAIRLEPNFVSAYIDRGNAYRATGDNDH
                     AIADFSEAIRLDPKSADAYYNRGLAHLYGGDLAKALADVSEASELDPQDAYKALWADI
                     VAQRNNAPSRLSQASMRIDMTPWPAPIVRSFLGRMPPEAVLAAADDPDAKKKKNQVCE
                     ANFYGGELALRLNAKDEAVRLFRLAANGCAKDVNEWFAANAELKALGETP"
     gene            1056..1313
                     /locus_tag="VM910_09175"
     CDS             1056..1313
                     /locus_tag="VM910_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62741.1"
                     /translation="MIAPALERAPRMLLVIGEDEQGLPTETIILEPSVSELYAALRAW
                     LQAVLPKWLGRALGLTRESHAPPLAACEPGLIDARLRWAGL"
     gene            complement(1406..3217)
                     /locus_tag="VM910_09180"
     CDS             complement(1406..3217)
                     /locus_tag="VM910_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI62742.1"
                     /translation="MLARTDTVATIAETWLARFEEALAAPARARLKGLFHPDSLWRDV
                     LALTWHIKTVNGADAILRELATHADRARPTGFKIDPHRTAPRNVRRAGTDAIEAIFSF
                     KTTEGRGSGVTRLTPDADDGNAFKAWTLLTALDEIKGHEERLGRSRPQGKAYSRDFHG
                     PNWLDLRKATAQYDDRDPTVLVVGGGQAGLSIAARLAQLGVDTLIVDREARIGDNWRK
                     RYHALVLHNQVHVNHLPYMPFPPNWPTYIPKDKLAAWFEAYVESMELNYWTATEFEGG
                     SYDEKAKRWSVVLRRADGTTREMHPRHVVMATGVSGIPSLPDIPSLRGFGGKVLHSSQ
                     YDDGEAWKGKNVLVIGSGNSGHDIAQDLHSAGAKVTLVQRSSTMIVNVEPSAQLQYAL
                     YDEGPSLEDCDLITTSMPLSLVRKSHIALTETARNMDKELLDGLERRGFRLDFGEDGT
                     GWQFKYLTRGGGYYFNVGCSDLIVKGEIGLAQFADIATFVAAGALMRSGETLAADLIV
                     LATGYKGQEHLVRKLFGDDVAARVGPIWGFGDGQELRNMFTRTAQPGLWFIAGSFAQC
                     RIYSKYLGLQIKACELGLLSRDVERQLRIRSRSEPVT"
     gene            complement(3466..4209)
                     /locus_tag="VM910_09185"
     CDS             complement(3466..4209)
                     /locus_tag="VM910_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62743.1"
                     /translation="MPSFKQLLSAPVLPAIFLTCLAVAAHADPVDCELNKVALARVVT
                     KDARLNFIAGRSERTPACPSAESACRLKAYLVPADEVLVNAADGAYVCAVFKSQAGTE
                     TRGWLPHAALQIAPPEPAPARQWDGKWERDREAQIVIKSHQDEVEVSGNAMWGSYDRQ
                     RVKRGAVHVGELNGKGRPRGHTLAIGFDPDRSGFPPAKDTAPDNCAAKLELYGRYLVV
                     EDDGMCGGLNVSFTGIYVRVKVLKRLGGV"
     gene            4621..4839
                     /locus_tag="VM910_09190"
     CDS             4621..4839
                     /locus_tag="VM910_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62744.1"
                     /translation="MKMFIKIALAAVIVVSAAAMASAATRSHKTAVYRSDPRAQATVP
                     RTPRFSNPDSPAATGGGSLGYNQNIYNW"
     gene            complement(5674..5847)
                     /locus_tag="VM910_09195"
     CDS             complement(5674..5847)
                     /locus_tag="VM910_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62745.1"
                     /translation="MAPKPDRLAFVFGMVLAMAVTILAITTWAAPPNPESDWAAPIPP
                     RETIYRPERWNLY"
     gene            complement(6334..6861)
                     /locus_tag="VM910_09200"
     CDS             complement(6334..6861)
                     /locus_tag="VM910_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI62746.1"
                     /translation="MPLPLIPNIAVFRRKLAGFPIATFQAGETVLTAASTTGRLLMLR
                     KGAVAVLKEGVEIATVTEPGAVFGELSVLLDQPHTADVRALETSQFYVADAATLLRVD
                     PVALLYVATVLARRLDDANQALVELRRQLEAGQPRSVIEKKAEEMEGLLVASGANLTY
                     ARYPYDLFAPDAANG"
     gene            7166..7840
                     /locus_tag="VM910_09205"
     CDS             7166..7840
                     /locus_tag="VM910_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62747.1"
                     /translation="MKRREFITLLGGAAAAWPLAARAQQTPRPPASGFLNPTTASVAN
                     QRVAAFVERLRAVGWIEGRAVAIKVRWAEGRSERAAAIVAEFARQKADLVVLRGTASV
                     LATREATSVIPIVFAGGDPVGTGLVVTLARPGGDVTGVSLQQADLAGKRLERLREVVP
                     ALRRLAILGNAGNPVTLRDLRAVEAAAGAPGITVVTVEIWRVEDMAAGFAALEARADM
                     FRPIRS"
     gene            8024..9142
                     /locus_tag="VM910_09210"
     CDS             8024..9142
                     /locus_tag="VM910_09210"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HVI62748.1"
                     /translation="MHRRHFGLSLAAAVLLAQQLREAAAQSASQSPSAGDTDSAWRMR
                     DAATAFLASLDQAARKAVSFPLEADQRTSWSNLPVALVPRVGMNVGALGLESRRRLHD
                     LLRASTSSQGYHKIAAIMRHDDVLRAAELEYLQHNPPRPRAGRNAIESMGSANYWIAI
                     FGEPARGQPWAWLLTGHHLGATFTSAGGRVTAAPLFLGAQPLEDLTEPYAGFVVLSHE
                     GLRGLDLVGSLHPQQTKVAVLSAEPLFSDVLTGVGRRNSLSRFEGLPASELDAPQKKL
                     LFALVEEYVRNADVDAAERHLDAIQRAGLDQLHFSWRGPTNDFRSPFYYRIHGPRLII
                     EFAVQEPNHIHTIMRDPQNDYGMDWLGLHYEEHAWNAR"
     gene            9605..>10303
                     /locus_tag="VM910_09215"
     CDS             9605..>10303
                     /locus_tag="VM910_09215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62749.1"
                     /translation="MRRREFITLVAGAAAAGPIVARAQTYPSRTITLVVPFPPGGVAD
                     FAARPLAAHLADTLGQKVVVENKGGAGGGIGHAYVARAEPDGYTIMTALPSLAVIPEG
                     NRLAGKPVPYEMDQFVPLARMFADPPILAVKNSSPWNSFNDFIAAVKANPGQIPYGTS
                     GHLGTVHLAMEMFLNAAQLKMVHVPYQGGGPSFNALLSGQVPVVPTLESTAKGQIDAG
                     NVRILAQWGTERLPN"
BASE COUNT         1861 a         3245 c         3236 g         1961 t
ORIGIN      
        1 gccatcgccg actttagcga ggcgatccgg ctcgatccca agttcgccgc cgcctacagc
       61 gaccgggcga ccgcgtacct cctccagggc aatagcgacc gcgccatcgc cgactttagc
      121 gcggcgatcc gcctcgatcc caagctcgcc tccgcctaca acgaccgggg caaggtgtac
      181 cagctgcaag ggaataacga ccgcgccatc gccgacttga gcgaggcgat ccggctcgag
      241 cccaacttcg tctccgccta catcgaccgc ggcaacgcgt accgcgccac gggcgacaac
      301 gaccacgcca tcgccgactt tagcgaggcg atccggctcg atcccaagtc cgccgacgcc
      361 tactacaacc ggggcctcgc gcatctctac ggcggcgatc tcgccaaggc tttggccgat
      421 gtcagcgagg cgagcgagct cgatccgcag gatgcgtata aggcgctgtg ggccgatatc
      481 gtcgcacagc gcaacaatgc cccgagccgt ctatcgcagg cgagcatgag gatcgacatg
      541 accccatggc ccgcgccgat tgtccgctcg ttcctcggtc ggatgccgcc ggaagccgtg
      601 ctcgccgccg ccgatgatcc cgacgccaag aagaaaaaga accaggtctg cgaagccaat
      661 ttctacggcg gcgaattggc gctgcggctc aatgcgaagg acgaagcggt gcgcctgttc
      721 cggttggcgg cgaacggctg cgcgaaagac gtcaacgaat ggttcgcggc caatgcggag
      781 ctcaaggcgc tcggtgaaac gccttgaacg aaccggcgcc gcatacacgc aaaaacacgc
      841 gccgcgacac gccttatgcc cgtgccagga aggcgctgcc caggaccgca cccgccgccg
      901 cgttgccatg cctttcggat tgaaaggagt gtggcaaagt aaacacacgt gatttttttc
      961 aagcgacgga acccgctttg gtcgctcgtc gcattgacga tcgaaatcaa aacgacaaag
     1021 gtagattttg tcaccctatt gcgggcgaga ccaccatgat cgcgccggcc ctcgagcgcg
     1081 cgccgcggat gctgcttgtg atcggcgagg acgaacaggg cctgccgacg gagacgatca
     1141 ttctcgagcc cagcgtgtcg gagctctatg cggcgctgcg cgcgtggctg caagccgtgc
     1201 tgccgaagtg gcttggccgc gcgctgggcc tgacaaggga gagccacgct cctccgcttg
     1261 cggcttgcga gccgggattg attgacgccc ggctgagatg ggctggtcta taaatatcgc
     1321 caagcgtgta gacggccgcg gcgccgcgct gaccggcgga cgatccgagc ggcgtcgccg
     1381 cttcgaggcg taaagccgac ctcgttcaag tgacgggctc agatctgctt ctgatccgaa
     1441 gctgacgttc gacgtcgcgg gacaggaggc ccaattcgca ggccttgatc tgtagaccga
     1501 gatatttcga gtagatgcgg cattgcgcga agctgcccgc gatgaaccaa aggcccggct
     1561 gcgcagttcg cgtgaacatg ttacgcaatt cttgtccatc gccgaatccc caaatcgggc
     1621 cgacgcgggc ggcaacatca tccccgaaca atttgcggac tagatgctcc tgccccttgt
     1681 agcctgtcgc cagcacgatc aggtcggcgg caagcgtttc gccgctgcgc atgagtgcgc
     1741 cagccgccac gaaggtggcg atatcggcga attgcgccaa tccgatctcg cctttgacga
     1801 tcagatccga acagccgacg ttgaaatagt agccgccgcc gcgggtgagg tacttgaact
     1861 gccacccggt tccgtcttcg ccgaaatcga gcctgaagcc gcggcgctcg agcccatcga
     1921 gcaattcttt gtccatgttc cgcgcggttt cggtcaatgc gatgtggctt ttccgtacca
     1981 gtgacagcgg catggatgtc gtgatgagat cgcaatcctc cagcgacggt ccttcgtcat
     2041 agagcgcgta ctgcagttgc gcgctcggct cgacattgac aatcatggtc gagctgcgtt
     2101 gcaccaacgt aaccttcgct ccggccgaat gcaggtcctg cgcgatatca tggccgctgt
     2161 tgcccgagcc gatgacgaga acgttcttgc ccttccacgc ctcgccatcg tcgtactgac
     2221 tggagtgcag aactttgccg ccgaagccgc gcaagctcgg aatatccgga aggctcggaa
     2281 tgccgctcac ccctgtggcc atcacgacgt ggcgcggatg catctcccgc gtcgtgccgt
     2341 cggcccggcg cagcacgacg gaccatcgtt ttgctttttc gtcgtagctg ccgccttcga
     2401 attcggtggc cgtccaatag ttcaactcca tgctctccac ataggcctcg aaccaggcgg
     2461 cgagcttgtc tttgggaatg taggtcggcc agttcggcgg aaagggcatg tagggcaggt
     2521 ggttgacgtg cacctggttg tgcaggacca gcgcgtgata gcgcttgcgc cagttgtcgc
     2581 cgatgcgcgc ttcgcggtcg acgatcagcg tatcgacgcc caattgcgcg aggcgcgcgg
     2641 cgatcgaaag cccggcctgt cccccgccga caacgagcac cgtcgggtcg cggtcgtcat
     2701 attgcgcggt cgccttgcgc aggtcgagcc agttaggtcc gtgaaaatcg cgcgaatagg
     2761 ccttgccttg aggccgtgat cttccgagcc gctcctcgtg tccctttatt tcgtcaagcg
     2821 ccgtcagcag cgtccatgcc ttgaacgcgt tgccgtcgtc ggcatcgggc gtgagccgtg
     2881 tgacgccgct gccacgaccc tccgtcgttt taaagctgaa gatggcttcg atcgcatcgg
     2941 tgccggcgcg cctcacattg cggggcgcgg tgcgatgcgg gtctatcttg aaaccagtgg
     3001 gacgggcgcg gtcggcatgg gttgcaagct cgcgcaaaat cgcgtccgcg ccgttgaccg
     3061 tcttgatgtg ccacgtcagg gccagcacat cgcgccaaag gctgtcggga tgaaacagcc
     3121 ccttgaggcg cgcgcgcgcc ggcgctgcga gcgcttcttc gaaccgcgcc agccaagtct
     3181 ccgcaatcgt tgcgacggtg tccgttctgg cgagcatcgg gcgctctcac gctgggcttc
     3241 ggcggaacag tcggttgcgg ccgaagctag catattcgcc gcagttgcgc aggctcgaaa
     3301 cacgccgcgt tcattgcagt cgggaggtct gcctccgtca atcgaatgtc cgctttgagg
     3361 ggcaaagcgg actcggcctg agggacggcg cgaggtccag gcataaccca atccgaccga
     3421 agtcgacaaa gacgatttca cttggtgcca gaaggccgct ttgggtcaaa ccccgcccag
     3481 tcgcttcagc accttcaccc gcacatagat tccggtgaag ctcacgttga gaccaccgca
     3541 catgccatcg tcctcgacca ccaggtaccg accatagagc tccagcttcg cggcgcagtt
     3601 atccggcgcg gtatccttcg caggcggaaa gcctgatcga tcgggatcaa aaccgatggc
     3661 gagcgtgtga ccccgtggtc tgcctttgcc gttgagttcg ccgacatgaa ccgcgcccct
     3721 cttgacgcgc tgccggtcgt agctgcccca catcgcgttg cccgagacct ccacttcatc
     3781 ttgatgggat ttgatgacga tctgcgcttc tcggtcgcgt tcccacttgc catcccattg
     3841 ccgcgcgggc gcaggctccg gcggcgcgat ttggagcgcc gcgtgcggca accagccgcg
     3901 cgtctcggtg ccagcttgtg acttgaacac tgcgcaaaca taggcgccgt ccgcggcgtt
     3961 cacgagaacc tcgtctgcgg gcacgaggta ggcttttagc ctgcaagcgc tctcggcgga
     4021 cggacaggcg ggcgttcgct cgctgcggcc ggcgataaag ttgaggcgcg cgtctttcgt
     4081 gacgacgcgc gcgagcgcga ccttgttcag ttcgcaatcg accggatccg cgtgtgccgc
     4141 aaccgcgaga caggtcagga atatcgccgg tagaacgggg gcggagagga gctgcttgaa
     4201 agaaggcatg ggcgtgatgc tgcgccgcca ttggcgatcg gacaagcccg aatcaaagct
     4261 gccctatgta gagacacgat ggccgattgc agcggtggct ctcgggtgcg aaggcgcagg
     4321 tgatgcgctt gttttgagtc tttcagcgtt cgctatccga cgcaggaggc ttcgcactcc
     4381 gttaccacct cgtgtcccgc ctcccaaccg ggctttctcc caaccccgca tcggtgcgat
     4441 gaagcgcaca cggagatttt atctccggtg atatggagtt tacgctcaag agattggaga
     4501 ttttatttct gccgatgcta gcgacgcagc gggcgaccga gcgaattcgt ccggcagcaa
     4561 cagcagcaac agcagattga gcccgaggaa gagcgtccga gcaaccacta gaacccggag
     4621 atgaaaatgt tcatcaaaat agcgcttgcc gctgtcattg ttgtcagcgc cgccgccatg
     4681 gcgtcggctg ccaccaggag ccataaaact gcagtgtacc ggagcgatcc gcgcgctcaa
     4741 gcgaccgtcc cgcgcacccc ccggttttcc aaccccgaca gtccggcggc cacaggcggt
     4801 ggcagcctcg gctacaatca aaacatctac aactggtgac ggatcgtatc gccggctggc
     4861 atcgatgttg gacgaatgct caagggcgaa acgcgttgct cgacgcgcat gacaccggca
     4921 tcaccttccc gctccgcctg ctcgcgcgca ccggccaggt gaacagaata ccgtattcgt
     4981 tgcagcgcat gtctttagct ttggcggtga acggacatgt tcgaggcaaa ctcgcgatgg
     5041 ccgctttgag gggggagcgg cctatggcct ctctctatta tgagggctgc cggcaacgcg
     5101 ggacgatccg tttttttgtc ccatttcgga cttctttgtt atcgaattcg taacacttgg
     5161 ccgtccaatg tgccggggag tgcccgagag ccgcggcgcg caagggaagc cgcgtgtggg
     5221 agagagtgct atggttagag ttctcaacga aatcttgctg tattccggca tggccgcatt
     5281 cgtcacgggg attgtcgtgc tcgcggcggc gacctggatc agctgaagcc gtcaccgaaa
     5341 tttcaggttg gtgcgcggtc acgaagccgc cgatccgcaa tcgagcaaca ccgttgctcg
     5401 ctttgtggtg cggggctcgc acgccagtgt ggaatcctgt ggcgacgcgg ttccagccat
     5461 tcaccgccgc gatggcgtag tgatgtcgga actcgctcgc cgtcatagtg acgcgtcagc
     5521 ggcgcataga tcgagttttt ccgctttcga cccccagcgg gcatcggcat cgcgcgcttc
     5581 gcgattgcga ttttcgtatt ggcagttcga atagcgattt gcgacggcgg ccgtcgtgaa
     5641 ggttcattcc gccggggctg cgtgccgacc ccttcagtac aaattccagc gttcaggccg
     5701 gtaaattgtt tcccgcggcg ggatcggcgc ggcccaatcg ctctcgggat tcggcggagc
     5761 tgcccacgtc gtgatcgcca ggatggttac cgccatcgca agaaccatcc cgaagacaaa
     5821 tgcgagcctg tctggtttcg gtgccatgtc ttcctcctgg cgccagatcg atgtgctgtg
     5881 tcggctctag aggtggagcg ggcaggtatc tgtgacgatc gtcacagttc ggtcgaatag
     5941 gaacaagcta tagcgcccac attgacggca tcggcgcagc cgatgtgcgc ccgcagcgcg
     6001 gtcgccgcat tgcgaacaga cggccgtcat aatcggctct ttgcgcgggg cttcgcctgt
     6061 ccgcctgcgg ggcgcaaagc ggacatgcgt cggcaatcgc cttcaatgac gacttgcgag
     6121 cccacgcttc ctggctcgct aacttgcaaa aatgtcacag tcattgattt tcagcgttcc
     6181 atgcttctgc gcagggaacc cgcagggccg tcccgtagat aattgacggc ggttgccggc
     6241 acggcctagg acgcaccgag accgccgggc cccggaggat gcaggtccgc cggggcctcc
     6301 cggccgtgcg tgtgactttt agcgagctgc gcctcagccg tttgctgcgt cgggggcgaa
     6361 caggtcgtag ggatagcggg catacgtaag attggcaccg ctggcgacca gcagtccttc
     6421 catctcctcg gcttttttct cgatcacact gcgcggctgg ccggcctcaa gctggcgcct
     6481 caactcgacg agcgcctggt tggcgtcgtc cagacgccgt gccaggaccg tcgcaacgta
     6541 aaggagcgcg accgggtcca ccctgagcag ggtggccgca tcggcgacgt aaaactgcga
     6601 ggtctccagc gcacgtacat ccgccgtgtg cggctgatcg agaagcaccg agagttcgcc
     6661 gaacacggca ccgggctccg tcaccgtggc gatctcgacg ccttccttga ggactgcgac
     6721 cgcgcccttc ctcagcatca acaaccggcc ggtcgttgag gcggcagtga gcacggtctc
     6781 gccggcctgg aaggttgcga tcggaaaccc ggcgagcttt ctccggaata cggcgatgtt
     6841 gggaatgagg ggcagaggca tgctcggtgc tccgttccgc gttcgagtta cgaccgtcca
     6901 gccttctcgt gtcgtagtgt gcaacactcg gcaaaaaagc gcatttttgt gatcgccatc
     6961 acatcgcgaa cgcaccggcg gcgctagcct cacgttcgtc acggtaccgc atgtgcggtg
     7021 ctcactatcg atggttcctc ccgaactcag ccccgccaac gcggggcttc tttgcgttct
     7081 gaaccccatc gatcgggagc attccggaag acacggggcg cgcatcatcc caaccgaatg
     7141 gttggcggga tggctggggg atcgaatgaa acggcgcgaa ttcatcacgc ttctcggcgg
     7201 tgcggccgcc gcttggccgc tcgcggcgcg cgcgcagcag acgccaaggc cgccggccag
     7261 cgggttcttg aacccgacga cggcttcggt tgcgaaccag cgggtcgccg ctttcgtaga
     7321 gcggctgcgc gcggtcggct ggatcgaggg ccgcgccgtc gcgatcaagg ttcgatgggc
     7381 ggaggggcgg agcgagcgcg cagccgcgat cgtcgcggag ttcgcccggc agaaggccga
     7441 cctcgttgtt ttaaggggaa ccgcaagtgt tctcgcaaca agggaggcga catcggtgat
     7501 ccccattgtc ttcgcggggg gagacccggt cggcaccggc cttgtcgtca ctctggcgcg
     7561 accgggtggc gacgtcactg gcgtatcgct ccagcaggcc gatcttgccg gcaaacgact
     7621 cgaacgcttg cgcgaagttg tccctgcgct tcgccggtta gcgattttgg gcaatgccgg
     7681 caatcccgtc accctccggg acctgcgtgc ggttgaggcc gcggccggcg cgcccggcat
     7741 taccgtggtc acggtggaga tttggcgcgt ggaggatatg gctgccggtt tcgcggcgct
     7801 tgaggctcgc gcggatatgt tccgcccgat ccgctcctaa gcacgcacca atcgaattcg
     7861 catcaatacc gtggcgccgg ccgcgcgact gccgacaatg cgccgttgac cggaacattt
     7921 cttacgagga tttaatgaga acgcaatgtc cgtgggcgcg agcaaacatt aggctgatcg
     7981 agatcaaagt ccgcgggctc attcaaaaat agaaagaggc gccatgcacc gacgacattt
     8041 cggcttgagt cttgccgctg cggtcttgct cgcccagcag ttgcgcgaag cggctgcgca
     8101 gagcgccagc cagtcaccgt ccgcggggga cacggattcg gcatggcgca tgcgggacgc
     8161 cgccactgcc ttcctcgcat ccctcgacca agcggcgcgc aaggctgtgt cctttccgct
     8221 cgaagcggat caaaggacat catggagcaa tctaccggtc gcgcttgttc cgcgggttgg
     8281 aatgaatgtc ggcgcgctcg gcttggagag caggcgccga ctgcacgatc tcctgcgcgc
     8341 gtccacgagc agccaaggct atcacaagat cgcagcgatc atgcgccatg acgacgttct
     8401 gcgggccgcg gagctggaat acctgcaaca taatcctccg aggccgaggg ccggacgcaa
     8461 cgcgatcgaa agcatgggat cggcgaacta ttggattgca atcttcggcg agccggcgcg
     8521 cggccaaccg tgggcttggt tgctgacggg ccatcatctc ggtgcgacgt ttacctctgc
     8581 cggcggccga gtgacggccg ctcccttgtt cctcggcgct caaccgttgg aggatctgac
     8641 ggaaccctat gccggttttg tggtgctcag tcacgagggg ctgcgcggtc ttgatctcgt
     8701 cggctcgctc catccgcagc agaccaaggt cgccgtcctg tccgccgagc cgctcttttc
     8761 cgatgtgctg acgggcgtcg gccggcgcaa cagcctgagc cgattcgagg gacttcctgc
     8821 cagcgagctc gatgcgcccc aaaagaaatt gctgttcgcg ttggtcgaag agtacgtgcg
     8881 caacgcggat gttgacgcgg ccgagcggca ccttgatgcc attcaacggg ctggcctcga
     8941 ccagttgcac ttttcgtggc gcggtccgac caacgatttt cgcagcccgt tctactatcg
     9001 gattcacggg ccacgcttga ttatcgaatt cgccgtgcag gagcccaatc atatccacac
     9061 catcatgcga gacccgcaaa acgactacgg aatggactgg ctcggcctgc actatgagga
     9121 acatgcgtgg aacgcaagat gatgacggag cccaactgat cggctccctt catcgcgact
     9181 gctgctgctg acctcggggc gaggtggtcc tcgatttatg acggacggcc gtgctgcata
     9241 ccacggagat gcggtcttgg tttagtcccg cccacgttct aacctacaag aggagtgcca
     9301 ccaatgaagc atcccgataa aggttgcgac tgcgctgcgt cggcttccgc gttggcgtta
     9361 cggtggcgct gccctgtctg tggcaagacc gcaatcatga aggtctcgac cctcgctgcg
     9421 gtttgcgacg gtgacacgct tggaaaggtt agacccgagg tcgtttcaca tcagaacacc
     9481 caggatcatg gccggtcctg ataatcaagt cgcgctactt tttgctgcgg cgcgttagga
     9541 cgcaattggc gtctcgcgtc agttcgttgc gacgcatcgg gtcggccgct atgggaggga
     9601 gcacatgagg cgccgtgagt tcatcacgct cgtcgccggc gcggcggcgg ccgggcccat
     9661 agtggcacgc gcgcaaacct atccgtcgcg cacaatcacc ctcgtggttc cgttcccgcc
     9721 cggcggcgtc gccgacttcg cggcccgccc gctcgccgct catctcgccg acacgttggg
     9781 acagaaggtc gtcgtcgaga acaagggcgg cgcgggaggc gggatcgggc acgcttacgt
     9841 cgcgcgcgcc gagcccgacg gctacacgat catgaccgcg ctgccttcgc ttgccgtcat
     9901 tcccgaaggc aatcggctgg cgggcaagcc ggtcccctac gagatggacc aattcgtgcc
     9961 gctcgcgcgg atgtttgccg atccgcccat cctggcggtg aagaactcat cgccgtggaa
    10021 ttcgttcaac gatttcatcg cggcggtcaa agcgaacccc ggccagatcc cctatggcac
    10081 ctccgggcat ctgggcaccg tgcatctggc gatggaaatg tttctcaatg cggcgcagct
    10141 caagatggtg cacgtgccgt accagggcgg cggcccgtcg ttcaacgcgc tgttgtcggg
    10201 ccaggtgccg gtggtgccca cgctcgagtc gaccgccaag ggccagatcg atgccggaaa
    10261 cgtccgcatc ctggcgcaat ggggcaccga gcggctgccg aat
//
LOCUS       DATLJT010000400         3590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_482480,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000400 DATLJT010000000
VERSION     DATLJT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482480"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM910_09220"
     CDS             <1..399
                     /locus_tag="VM910_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI62750.1"
                     /translation="YEPAPRAHGVTTFNVMRLLGLSIEGLTSFSVAPLRFASLLGVLL
                     ASAAFLFGLSILWEVFTTGKQVPGYPSLMVGLMTIGGVQLIMIGIVGEYIGKILSELK
                     ARPIYFVAEHTEKRAEADGASSASERTAAE"
     gene            396..1223
                     /locus_tag="VM910_09225"
     CDS             396..1223
                     /locus_tag="VM910_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HVI62751.1"
                     /translation="MKAIWLCADDYGISPGVNRAIRDLIGKGRLNATSVMVVGPATGR
                     DEVAGLLDAAKDRPRCAIGMHATLTAPFRPLTMHFRPIDGGMFLPFPRLLRAGLLHRL
                     DPEIVRAELVTQLAAFRDMFGRSPDFVDGHQHVQLYPQVRDGFLAAVKEKAPDAWVRQ
                     ARRNQPLLKRLGAPKPLFLDILSAQFARRTARAGLAANPAFAGAYDYSRKPDYGALMR
                     QFLDGLPAHGVVMCHPGFVDDVLVGLDPLTTTREIEHAYLASDAFPRLLAENDFTLD"
     gene            1357..2163
                     /locus_tag="VM910_09230"
     CDS             1357..2163
                     /locus_tag="VM910_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HVI62752.1"
                     /translation="MTPQERQLIDELFDRLSRLEGTPRDPEASAAIAQGLRSAPNAIY
                     PLVQTVLVQDEALKRANQRIQELEQALAGGQDQSQSGGFLDSMRDKIFGQQGQSRGPG
                     SVPNVPPPLPQNRPVWNSGQAMQQVQSPPSWGQQPYGGPQSPVGGGGSFLGTAAATAA
                     GMVGGSLLLNSIRGMMGGGQHQAFGDTTTSNERSSSPWSNQSDTPMARDLGVDDVGRT
                     ADRDDDNSRSGLFDQASYDPSDDQDDMDHDSDDFGGNDDFGGNDDGSDYA"
     gene            complement(2235..3035)
                     /locus_tag="VM910_09235"
     CDS             complement(2235..3035)
                     /locus_tag="VM910_09235"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI62753.1"
                     /translation="MFRHLSLALLAGATCLGAEINQAAAFDGAPLQEPSVIYAGEPQA
                     SAPMRSPYADRSDLGGGFIEFLFGDSPAQGRYRSQDSQRPIYQQQPGYEPQRLLPPMA
                     PQYMRLDEYRQLMQRPFDPRYQRQEVEYYGRERAGTIVIDTPNKFLYLVEGNGRALRY
                     GVGVGRPGFAWAGVKTITAKREWPDWTPPAEMLVRRPDLPRHMEGGPENPLGARAMYL
                     GSSLYRIHGSNEPWTIGTNVSSGCIRMRNEDVIDLYGRVNVGTRVIVL"
     gene            3330..>3590
                     /locus_tag="VM910_09240"
     CDS             3330..>3590
                     /locus_tag="VM910_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HVI62754.1"
                     /translation="MPVHRKRFRIEEAIGVELPRPEADEAGPMHREIMAELRAIRAQM
                     GMPGRSATGIEGQVAREAAEAHVLLETYRAQIEQCEKLKVELD"
BASE COUNT          629 a         1170 c         1132 g          659 t
ORIGIN      
        1 tacgagccgg cgccgcgcgc ccacggcgtc accactttca acgtgatgcg gctcctcggc
       61 ctttcgatcg agggactgac ctcgttctcg gtggcgccgt tgcgctttgc gagcctgctc
      121 ggcgtgctcc tcgcgtccgc cgccttcctg ttcgggctct cgatcctgtg ggaggtgttc
      181 accaccggca agcaggtgcc cggctatccc tcgctcatgg tcggcctgat gaccatcggc
      241 ggcgttcagc tcatcatgat cgggatcgtc ggcgaataca tcggaaagat cctctccgag
      301 ctgaaggcgc ggccgatcta ctttgtcgcc gagcacaccg aaaaacgcgc cgaggcagac
      361 ggcgcctcaa gcgccagcga acggaccgcc gccgaatgaa ggcgatctgg ctgtgcgccg
      421 acgattacgg catcagccct ggcgtcaacc gcgccattcg cgacctgatc ggcaagggcc
      481 gcctcaacgc gacctcggtg atggtggtcg gccccgcgac cggccgcgac gaggtcgcag
      541 gcctgctgga tgcggccaag gatcggccgc gctgtgcgat cgggatgcac gccacgctga
      601 ccgcgccatt tcgtccgctg accatgcatt tccgcccgat cgacggcggc atgtttctgc
      661 cgtttccgcg gctgttgcgc gcagggctat tgcaccggct cgatcccgag atcgtccgcg
      721 ccgaactggt cacgcagctc gccgcattcc gcgacatgtt cggccggtcg cccgatttcg
      781 tcgacggcca tcagcatgtt cagctttatc cgcaagtccg tgacggcttt ctcgccgcgg
      841 tgaaggagaa ggcgcccgac gcctgggtgc gccaggcccg acgtaaccag ccgctcctca
      901 agcgcctcgg cgcgccaaag ccgctgtttc tcgatatcct gagcgcacag ttcgcgcggc
      961 gcaccgcccg tgcgggtctt gccgccaatc ccgctttcgc cggcgcctat gactattcgc
     1021 gcaaacccga ctacggcgcc ctgatgcggc agttcctcga cggcctgcct gcgcacggcg
     1081 tcgtcatgtg tcaccccggc tttgtcgatg acgttctggt cggcctcgat ccgcttacca
     1141 ccacgcgcga aatcgagcat gcctatctgg cgagcgacgc ctttccgcgt cttttggccg
     1201 aaaacgactt caccctggac tgactcggtc gccggccgaa ggccatgccc tggtggcgtg
     1261 gtgtcgcata cctaaattta atctcagggc ctgctaggcg cgacacaaac cgtccctaca
     1321 tttcggccgc gattcgaggt gagcgaggag gacgccatga caccgcaaga gcgccaattg
     1381 atcgacgagc ttttcgaccg gctttccagg ctggaaggca ccccgcgcga tcccgaggct
     1441 tccgccgcca ttgcccaagg cttgcgcagt gcgccgaatg ccatctaccc gctggttcag
     1501 accgtgctgg tgcaggacga agccttgaaa cgcgccaatc agcgcatcca ggaactggaa
     1561 caggcgctgg ccgggggaca ggatcagtcg caatccggcg gtttcctcga ttcgatgcgt
     1621 gacaagatct ttggccaaca gggccagtcg cgcggtccgg gctcggtgcc gaacgtaccg
     1681 ccgccccttc cccaaaaccg tccggtctgg aacagcggcc aggcaatgca acaggtccag
     1741 tcgccgccct cctggggcca gcagccttac ggcggcccgc aatcgcccgt cggcggcggc
     1801 ggctcgtttc tcggcactgc cgcggcgact gccgccggca tggtcggcgg atcgctgttg
     1861 ctcaacagca tccgcggaat gatgggcggc ggccagcatc aggccttcgg cgacaccacg
     1921 accagcaacg agcggtcgtc gagtccctgg agcaatcagt ccgacacccc gatggcgcgc
     1981 gacctcggcg tcgacgacgt cggtcgcaca gcggatcgcg acgacgacaa ttctcgctcc
     2041 ggcctgttcg accaggcgtc atacgacccg tctgacgatc aggacgacat ggatcacgat
     2101 tccgacgatt tcggcggcaa cgacgacttc ggcggcaacg acgacggcag cgactacgct
     2161 tgagcctcgt tcacgcctca aaagaaacgg ccgctcgaac gagcggccgt tttgttttcg
     2221 cggccttgcg gtgatcagag cacgatgacc cgcgtgccga cattgacgcg gccataaagg
     2281 tcgatcacgt cctcgttgcg catgcggatg cagcccgagg agacgttggt gccgatggtc
     2341 cagggctcgt tggagccatg gatgcgatag agcgaggagc ccagatacat cgcgcgcgcg
     2401 ccgagcggat tttccggacc gccctccata tgacgcggca ggtcgggacg gcgcaccagc
     2461 atctccgccg gcggcgtcca gtccggccat tcgcgcttcg ccgttatcgt cttgacgccc
     2521 gcccaggcga atccggggcg gccgacaccg acgccgtaac gcagcgccct gccgttgcct
     2581 tcgaccagat acaggaattt gttcggcgta tcgatgacga tggtgccggc gcgttcacgg
     2641 ccgtaatatt cgacttcctg cctctggtat ctcgggtcga acggacgctg catcagttgg
     2701 cgatactcgt ccagacgcat gtattgcggg gccatcggcg gcaggaggcg ctgcggctcg
     2761 taacccggct gctgttgata gatcggccgt tgcgagtctt gcgaccggta gcggccttgt
     2821 gccggcgaat cgccgaacag aaattcgata aagccgccgc cgagatcgga gcgatcggca
     2881 tagggcgagc gcatcggagc agaagcttgc ggctctccgg cgtagatcac gctgggctct
     2941 tggagcgggg cgccgtcgaa ggcagcggct tgattgattt ctgcgccgag gcaggtcgcg
     3001 ccggcaagca gcgcgagaga aagatgtctg aacattcgac gtactctgta cttttggtct
     3061 tgatcgcgcg cgagctttag cgcccgcgta tggctaatta agtccaaaac cgcctcggtt
     3121 tggtaaacgc aaacgccgtt tcgattcacc acgtccgcgg tgaggcgcgg atttttccgc
     3181 tagcgtttat tttcgatgaa gggcgatcgt gcatggttaa tcgatcgtct gcggcacttt
     3241 tggaatctgc gatcgaggtt ccgcgcgtga accggctgtt aaacgccgtc cgtctagaac
     3301 tccaacgagt tgggttcggg aagagtgcca tgcctgttca tcgcaagcgt tttcgtatcg
     3361 aagaagccat cggcgtcgag ctgccaagac cggaggccga cgaggccggt cccatgcatc
     3421 gcgagatcat ggcggagctg cgtgccatcc gcgcgcagat gggcatgccg ggaagaagcg
     3481 caaccggcat agaaggccag gtagcgcgcg aggccgcgga agcgcatgtg cttctggaga
     3541 cctatcgcgc ccagatcgag cagtgcgaga agctcaaggt cgagctggac
//
LOCUS       DATLJT010000401         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_711717,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000401 DATLJT010000000
VERSION     DATLJT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711717"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VM910_09245"
     CDS             complement(<1..1406)
                     /locus_tag="VM910_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI62755.1"
                     /translation="MLVERRGIPVSKDALMEAAWAGLTVEESNLAVQIAALRRVFGEE
                     PGGQNWIETLPRRGYRFIGPVSIRDQSAPAVVSLAANFPGSSNPTLPDRPSIAVLPFQ
                     NISADPEQEYFADGMADEIITALSRFPSLFVIARNSSFTYKGRAVDVKQIARELGVRY
                     VLEGSVRKAGNRVRFTGQLIDATTRGNIWADRFDGALEDVFDLQDQVTARAVGAIEPR
                     LQRAEIERAGRKPTESLDAYDYFLRGMASFHLFNRDSLLEAARLFVRATELDPNYASP
                     YGIASWSGALRNSYGWMVDREREIADAVRLARRAVALGKDDPTALWSGGLSLAYLGHE
                     VEAGAAYIDRALVLNPNSAISWTASGSVRMYLGESASAIEHFERAMRLSPLDPLTYFG
                     CTGMALAYACAGRYDEARSWATKALHEQPNWATALRVAAVANVLSDRMVEARAAMACL
                     REVDPALRLGNVDRVAPP"
     gene            1695..1910
                     /locus_tag="VM910_09250"
     CDS             1695..1910
                     /locus_tag="VM910_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62756.1"
                     /translation="MRKISLVAAIALMLAGVGVWATSTTQARLDVRAGAQINPLQIMK
                     GARHLPVAHYDDYSLEHEPDGLPDCSN"
     gene            1973..2557
                     /locus_tag="VM910_09255"
     CDS             1973..2557
                     /locus_tag="VM910_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62757.1"
                     /translation="MLNSAAIARPLRVSELYRDPCRVDDAGPKVVQFLDRVRDQNANP
                     TSPLDRYIEGWGQADLEKILEATAPSYRFTDPFVGSFDARSLHKYFDLVRNRLSCTGA
                     ISRRELTFFLQGPMKLRSPRELQFWREAPRIGLTGVAEIEIGERGVVAERVAYDLNLA
                     SEILCRPSALRSPVSRIGPRPLKALGEHAAALEI"
     gene            2599..2856
                     /locus_tag="VM910_09260"
     CDS             2599..2856
                     /locus_tag="VM910_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62758.1"
                     /translation="MTIQFSKQPIAVPSEGLPKMASGLVCRLVCARDDPGKQRIRMWL
                     IDLDDAQLRSGLGLTVEDIAVLRTGTKTTRPCVKKNDSGST"
     gene            2859..>3452
                     /locus_tag="VM910_09265"
     CDS             2859..>3452
                     /locus_tag="VM910_09265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62759.1"
                     /translation="MSITLAAREARPASSLVLLTMSLGVLIAQIDTSVVNLAVKPIGT
                     DLKASVTELQWIVDVYNFVYASLLLTAGTLADLYGRRCIFGLGIALFTLGTVVCGLAP
                     DVPTLVAGRAVAGLGAALEVPASLAILAIAYPDTKERTRALGIWASCYGIAIVIGPTA
                     GGALVDASGWRSIFLLIIPFCLATLVLVMTVVPESSNP"
BASE COUNT          688 a         1111 c          995 g          658 t
ORIGIN      
        1 aagggcggcg ctacccggtc aacattgcca agcctcaacg cgggatcgac ctcgcgcaag
       61 caggccatcg ccgcccgcgc ttcgaccatt cgatccgaaa gaacattggc aaccgccgcc
      121 acccgcagtg ccgtggccca gttcggttgc tcgtggaggg ctttggtcgc ccaggatctc
      181 gcctcgtcgt aacgaccggc acaggcatac gccaaggcca taccagtgca accaaagtag
      241 gtcagcggat caagcgggct cagccgcata gcacgctcaa aatgttcaat agcactggcg
      301 gactcgccaa ggtacatgcg caccgagccg ctcgcagtcc aactaattgc cgaattcggg
      361 ttcagaacca gcgcccggtc gatgtatgcg gcaccagcct cgacctcatg acccagataa
      421 gccaggctaa ggccgcctga ccacagcgca gtcggatcat ccttgcccaa cgcgaccgcg
      481 cgacgcgcca gcctcacggc gtcagcgatc tcacgctcgc gatcaaccat ccagccatag
      541 ctgttcctga gcgcgcccga ccacgacgcg atcccgtagg gagaggcgta attggggtcg
      601 agttccgtcg cgcgtacgaa gagccgcgcc gcttccagca ggctgtccct attaaacaga
      661 tgaaaactgg ccattccgcg caggaagtag tcgtaagcgt cgaggctctc ggtcggcttg
      721 cgtcccgcac gctcgatctc cgcgcgttga agccgcggct caatggcccc caccgccctt
      781 gcggtcacct gatcctgcag gtcgaatacg tcctcgagag caccatcgaa gcgatccgcc
      841 caaatgtttc cgcgggttgt cgcatcgatg agctgcccgg tgaaccgcac gcgatttccc
      901 gccttgcgga cgcttccctc gagcacgtaa cgcaccccga gttcgcgcgc gatctgtttg
      961 acgtcgacgg cccggccctt gtaagtgaag ctggagttgc gcgcgataac aaacagcgac
     1021 ggaaagcgcg acagcgccgt gatgatctca tcggccatgc catcggcgaa gtactcttgt
     1081 tctggatccg cgctgatgtt ctgaaacggc agaacggcga tggatggcct gtctggaagc
     1141 gtggggttcg aggaaccagg aaaattcgcg gccagcgaca caactgcggg ggcactttga
     1201 tcccggatgc tgaccgggcc gatgaaccga taccctcgtc tgggcaacgt ctcgatccag
     1261 ttctggccgc caggctcctc cccaaacacc cgacgtaggg ctgcgatctg cactgcgagg
     1321 ttgctctcct cgacagtgag accggcccac gcggcttcca tcagcgcgtc cttggaaacc
     1381 gggatacccc gtcgctccac cagcacgcgc agcaatgcga cggcacggtg acccagcgaa
     1441 acgggctcgc caccacggaa tagcgtgtcc gtctccgcat caaggcggaa ggggccgagg
     1501 atgtacgctg ctcccatctc ccgatctggc gccagttcag aaacgcttaa gaagtttttc
     1561 agctcagctt catgacttcg gaccggtccg ggctcagatt gctacggccc gacgacggac
     1621 gcttcacatc atggtgcgga tcgtgcgcca aggcaaggag atcgctcggt attcgagctc
     1681 tcggagaaac tcaaatgcgc aagatcagtt tggtcgccgc aattgcgttg atgctcgctg
     1741 gtgtcggagt ttgggccacc tcgaccactc aggcgcgtct cgacgtgcga gccggcgcac
     1801 agatcaatcc gttgcagatc atgaagggcg caaggcacct gcctgttgcc cattatgacg
     1861 attactccct ggagcatgag cctgacgggc tgccggactg ctccaattga gcgcccggcc
     1921 ctgttggcgc gtgaaacccg aacacggaaa ttcgtcagga ggagtacagg tcatgctgaa
     1981 ttctgcagca atcgcacggc cactacgagt ttcagagctg tatcgcgacc cgtgccgcgt
     2041 ggacgatgcc ggcccgaaag tggtgcagtt cctcgataga gtccgcgatc agaacgcaaa
     2101 cccgacttca ccacttgatc gatacatcga aggttggggg caggccgact tggaaaagat
     2161 cctcgaggcg accgccccga gctatcgctt caccgatccg ttcgtcggaa gtttcgatgc
     2221 ccggtcgctg cacaaatact tcgatctcgt gcggaacaga ctctcctgta caggagcaat
     2281 cagtcggcgc gagctcactt tcttcctgca aggcccaatg aagctgcggt cgcctcggga
     2341 acttcaattc tggcgcgagg cgccgcggat cgggctcacc ggggtggccg agatcgaaat
     2401 cggagaacga ggcgtcgtag ccgaacgcgt tgcatacgac cttaacttgg cgtccgaaat
     2461 attgtgccgc ccctcggcct tgcgctcacc agtctctcgc ataggcccgc gcccactcaa
     2521 agccctcggc gagcacgccg cggcgctgga aatttgacga aactccgcaa cttcactcct
     2581 ccgcgccatg agacggtcat gaccattcag ttctcaaaac aaccgatagc agtgccttct
     2641 gagggcctcc ccaaaatggc gtcaggcctc gtttgcagac tcgtttgtgc ccgcgacgat
     2701 cccggcaagc agcgcatccg catgtggctc atcgacctcg atgacgctca actgcggtcg
     2761 ggcctcggcc tgacggtgga agatattgct gtcctgcgca ctggtacgaa aacaacacgt
     2821 ccttgcgtta agaaaaacga ttctgggagc acgtaaaaat gtccatcaca ctcgccgcca
     2881 gagaagcgcg tcccgcaagc agtctagttc ttctcaccat gtcgctcggc gtgttgatcg
     2941 cgcagattga cacgtctgtc gtcaatcttg cggtcaaacc gatcggcacg gatttgaagg
     3001 ccagcgtgac tgagctgcag tggatcgttg atgtctacaa tttcgtctac gcaagcctgc
     3061 ttctcacagc cggaacactc gccgatctgt acggacggcg ttgcatcttt gggctcggca
     3121 tcgcgctttt cacgttggga acggtggtgt gcgggctagc gccggacgtg cccaccctcg
     3181 ttgccggccg cgccgtagcc ggtcttggcg ctgccctgga agtgccggcc tcccttgcga
     3241 tcctggccat cgcctatccg gatacaaagg aacgcacccg cgcgctggga atctgggcga
     3301 gctgctacgg gattgccatt gtcattggtc cgaccgccgg cggcgcgctt gtcgatgcat
     3361 ccggctggcg cagcattttc cttctgatca ttcccttctg cctcgcgacg ctcgtcttgg
     3421 tcatgaccgt ggtgccggaa tcgagcaacc cg
//
LOCUS       DATLJT010000402         7534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2780513,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000402 DATLJT010000000
VERSION     DATLJT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780513"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..1228)
                     /locus_tag="VM910_09270"
     CDS             complement(386..1228)
                     /locus_tag="VM910_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62760.1"
                     /translation="MTPDATSVVTAVRAVLTAQEVWQGTATELLHALAAADGTLALPG
                     NARAMAAHLRGVAPLLREIGIEMSFVRSGHAGTRTIHLALAAHAHATQLCIPDVLTQA
                     DEVNAQLGLTSPLPVDPSRPSDSRQLTQAVSDAPDTLAQAADANDVSLIADANSTSEV
                     LTQASDARADATEPTATDTLTQTAAPDEAAASAEALTQVAPTPSLTFEQHIVRWLDDH
                     PEPSEPGRCAYCGEMETRGRPVVPFETAPSTHAWLHSGCWRAWRGQRRFRAVIAIGGG
                     KLPG"
     gene            complement(1280..2224)
                     /locus_tag="VM910_09275"
     CDS             complement(1280..2224)
                     /locus_tag="VM910_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62761.1"
                     /translation="MSSNCRFTVDEERYIIDAVNADNSRKIHCYFEDEAKDDKRLRKR
                     VLMVRLGEMLEKLIVNLRPMEKLPSGEEYKPQNKESLAIEWTMLETISASHLNTFDPV
                     LSIQHKFDGKLRRQRKHTQDFTSLALFRDDVEFLLRDLYGDVDSDALDSVLWAVFRIV
                     EQFAKFVRPDLWKEHNDAVRLDYMFGIGVPEQGSIGGSDHRRGMVAKFDLIFRACEVA
                     REPSAFSEYTVEFANWFREQWYCNPESLLMMMGPPRSKMNTEDSFATSRWPRDADVPT
                     IARCILRTLKYEPHTDDTTPLRRALETLIDHELATASY"
     gene            complement(2445..2969)
                     /locus_tag="VM910_09280"
     CDS             complement(2445..2969)
                     /locus_tag="VM910_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62762.1"
                     /translation="MNAVQALEAAHAAGIHIELDGDQLVLEAPAAPPNCVVDLLARHK
                     FGIIALLRPHRDGWSAEDWQAFFHLKRTEAETQAFECCVIEWLNQNPAPSAAGQCGWC
                     RRAQTCDAVVLPFGTEPGTHAWLHAECWKAWHHARRAEAAAALTQLGIRKWHGTTAAP
                     ECVCVPDDAGHAEL"
     gene            complement(2966..5128)
                     /locus_tag="VM910_09285"
     CDS             complement(2966..5128)
                     /locus_tag="VM910_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62763.1"
                     /translation="MSEEAMTASVADETPDPSALNTRSEKLRPIIESHKADVAALQDT
                     RWSGVNSAESIVSDQERCALSPTNDPVEPGNAAEDIPDPPGHELSPQLEGVVETTAAN
                     PGAAFTPEVLQRLAELKKKDRATFEVLRRQLKDAGCRVTALDQAIDEERGYVGGRGPS
                     HADILIELAHAAELFHAPDSTGFADIDINGHRETWSIRSKGFRRWLARRFFEATQGAP
                     SSDALQSALNVLEAKAHFDSPERAVHVRVGGLGDRLYLDLCDEHWRAVEIDATGWRVI
                     DNPPVRFRRAAGMQALPMPVPGGSVETLRSFLNLRTDTDFVLAVAWILACLRNLGPYP
                     VLVLSGEQGSAKSTFSTILRALLDPNTAPLRALPREDRDLFIAASNGHVLAFDNVSGL
                     QTWISDTLCRLATGGGFAVRQLYTDQDEVLFDATRPVILNGIEDIVTRPDLADRAVFL
                     MLEPIPDESRRPEAELWAAFEIERPRILGALLDAVIEGLKRLSETHLPKLPRMADFAL
                     WATACETAVWPPGTFWSAYCGNSDEAVDRVIDADPIAAAVRAFMMARTVWTGTASVLL
                     DDLEDMAGQRIAKSKTWPNSPRALAGGLRRAATFLRKVGIECRFDREGRRRTRMIHIT
                     ATPSLAAPEQPGVQPSAPSASSASIMKSNPTKGFTAPSPRTVANDADGRGENRDATVR
                     VNLLKSKDADDADGVDASHPSQSAQEKTRTAGRWSARL"
     gene            complement(5121..5315)
                     /locus_tag="VM910_09290"
     CDS             complement(5121..5315)
                     /locus_tag="VM910_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62764.1"
                     /translation="MRASTTTDTPTLAFRVKPFCKNIGIGVTKFYELVKAGKIHTVVI
                     GGRRVIPVGEAERILREGCE"
     gene            complement(5419..6042)
                     /locus_tag="VM910_09295"
     CDS             complement(5419..6042)
                     /locus_tag="VM910_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62765.1"
                     /translation="MPTSIDGPTGQFAIDEIIRTEHANRRLAPDWQLSDAVRFGRRAL
                     VAAIKVAKSFERTPLDRYLEYWRGEAERAERAREALQALINYWKVNHEVRVMKDADFG
                     LTVAHDVCAALAKQSSENVRTQGRNEGDRPKREFVLRLAVAWMLLTGRPPAGGKDTFL
                     GFVDAAWLDAGFANEEDFTRSLQFAQKRLKKMSGADFITEASLLGLV"
     gene            6383..6826
                     /locus_tag="VM910_09300"
     CDS             6383..6826
                     /locus_tag="VM910_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62766.1"
                     /translation="MRGLVFALALVGAAATSARAQDAIPDLKGTWSGKGKSIVFGTNP
                     HHPGAQTAADPPRVRDIEATYIVDGQDGRLAWGRSSSTAANTQEPFAWAIASDNKTIV
                     GADMDGYFRITLISPDSIEKCYTHNGTSPTHAIVATCHVMNRVSR"
     gene            7223..>7534
                     /locus_tag="VM910_09305"
     CDS             7223..>7534
                     /locus_tag="VM910_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI62767.1"
                     /translation="MTRAMELELLDSKKKMISETYLRNAWYVAAWSDDLADGQLLSRT
                     ILKEPVVLFRKSDGHLAALQDRCPHRFAPLHMGKIIDGDRVQCPYHGLEFDSSGACVL
                     NP"
BASE COUNT         1437 a         2329 c         2228 g         1540 t
ORIGIN      
        1 tcggggtcgc atagcgtccg caccaaaccg gccaaggcaa ggtgtgtgta atcccgagcc
       61 cgcagcgcga tcggccgagg gtcgaacagc cgcactcctg gcatctctgg catgggaagc
      121 tttcgtctgc cgggcttggc accggctcca gggcggcggc ctccccaacc gggatatttc
      181 ggattaggag ccattgcggc aacgaaaatc ttgatccaat tggtcaagtt tcaagtttac
      241 gcccaagctg acaattcttc aataacgatc gtatgttgtg gcgcagtttc ggactgcggt
      301 aacttgatca aaacaacacg actcgccgtg ccagccctgc ccgttggttt gctgtctaca
      361 ggatggcgtt cataacgaag aaaaatcaac cagggagctt tccgccgcct atagcgatca
      421 ccgcgcggaa acgtcgctgg ccgcgccacg cgcgccagca gcccgaatgc agccacgcgt
      481 gcgtgctggg ggccgtctcg aatggcacga ctggccggcc cctggtctcc atctccccgc
      541 agtacgcgca acggcccggc tccgacggtt cgggatggtc atcgagccag cgaacaatgt
      601 gctgctcgaa cgtcagcgac ggtgtggggg caacttgcgt cagcgcctca gcagatgcgg
      661 cagcttcatc gggcgctgcg gtttgcgtca gcgtgtcggt agcggttggc tctgtggcgt
      721 cagcacgcgc gtcagacgct tgcgtcagca cctccgacgt cgagtttgcg tcagcgatga
      781 gtgagacatc gtttgcgtca gcggcctgcg ccagcgtgtc cggcgcgtcc gaaaccgcct
      841 gggtgagctg gcggctgtcg ctgggccgag aagggtcgac aggcagaggg gaggtgaggc
      901 cgagctgagc attgacctcg tcggcttgcg tcagcacgtc gggaatgcag agttgggtag
      961 cgtgcgcgtg agcggccaac gcgaggtgaa tggtcctggt gcccgcatgc ccactccgca
     1021 cgaagctcat ctcgatgcca atctcccgca gcaagggtgc cacgcctcgc agatgggcgg
     1081 ccatcgccct ggcgttgccc ggcaatgcga gcgtgccatc cgcagcagcg agcgcgtgga
     1141 gcaactccgt agccgtgccc tgccatacct cctgcgcggt tagcaccgcg cgcactgccg
     1201 tcactaccga ggttgcgtct ggcgtcatgt catacgcctt cgcaacgagc ccccaagatc
     1261 ggcgctgctc caaaccaggt taatatgacg cggtcgcaag ttcgtggtcg atcaaggtct
     1321 cgagggctcg ccgcagcggt gtggtgtcgt cggtgtgggg ttcatacttc agcgttctca
     1381 gaatgcagcg cgcgatcgtg gggacgtcgg cgtcgcgagg ccaacgggag gtggcgaagc
     1441 tatcttccgt attcattttc gagcgcggtg ggcccatcat catgagaaga ctctctgggt
     1501 tgcagtacca ttgctccctg aaccaatttg caaactcgac cgtgtactca ctgaatgcgg
     1561 acggttctcg tgcaacctcg caagcacgga aaataagatc aaattttgcc accattccgc
     1621 ggcgatggtc actgccgcct atcgaacctt gttctggcac accgatcccg aacatgtagt
     1681 caaggcgcac ggcgtcgtta tgttccttcc aaagatcagg gcgcacaaat ttcgcaaact
     1741 gttcaacgat acggaacacg gcccacagaa cgctgtccag tgcgtcgcta tcgacgtcgc
     1801 catataggtc gcgcagaaga aattcaacat catcgcggaa aagcgccaag gaagtgaaat
     1861 cctgggtgtg tttcctctgg cgtctgagtt tgccgtcgaa cttatgctgg atagacagca
     1921 cagggtcgaa cgtgttcaag tgcgaggcgc taatcgtttc gagcatcgtc cactcgattg
     1981 cgagagattc tttattctgt ggcttgtatt cttcgcccga cggcaacttt tccataggcc
     2041 tcaaattaac aatgagtttt tcgagcatct caccgagacg gaccataagt acgcgctttc
     2101 gaagacgctt gtcatctttc gcttcgtctt caaagtagca gtggatcttc cgcgaattat
     2161 cggcattgac tgcatcgatg atgtagcgct cttcatcaac ggtaaagcgg caattgctac
     2221 tcatgtgttt cctcctcatt gagtgctgcc ctgttcacgc gcgccggcat tctcgaaacg
     2281 aaccatcttc agacgctcga caagaaccgc acagtcggga acgacctgcc gttgcctttc
     2341 gacggacatg atctcatcac cccttcaacg taaccgttcg aaagtttcgc tgcatgaatt
     2401 cgccgtgtga tcgaggcgcc acatgctctc accacacgct gtcatcagag ttccgcatgg
     2461 cccgcgtcgt cgggaacaca gacgcactcc ggcgcggcgg tggtgccatg ccacttgcgt
     2521 atacctagct gcgttagcgc ggctgctgcc tcagcacgtc gtgcgtgatg ccatgccttc
     2581 cagcattcgg cgtggagcca ggcatgcgtc ccaggctctg tcccaaaggg caacacaacg
     2641 gcatcacagg tttgggcgcg ccggcaccac ccgcactgac ctgctgctga tggggccggg
     2701 ttttggttga gccattcaat gacgcagcac tcgaatgcct gtgtctccgc ttccgtgcgt
     2761 ttcagatgga agaatgcctg ccagtcctcg gctgaccaac cgtcccgatg gggccgcagc
     2821 agtgcaataa ttccgaactt atgacgcgcc aggaggtcga ccacgcaatt gggcggtgcc
     2881 gctggtgctt caagcaccaa ttggtcacca tcaagctcga tgtggatacc ggcggcatgc
     2941 gccgcctcga gggcttgaac cgcgttcata gccgcgcact ccagcgccca gctgtcctgg
     3001 ttttttcctg cgcggactgg gagggatgac ttgcgtccac accgtccgca tcgtccgcat
     3061 ctttggattt caataggttg acgcggacgg ttgcgtcccg gttttcgccc ctaccgtccg
     3121 cgtcgttagc aaccgtccgc gggctaggtg ctgtgaaccc tttggttggg ttggatttca
     3181 tgattgacgc ggacgatgcg gacggtgcgg acggttgcac cccgggttgt tctggtgcgg
     3241 caaggctcgg tgttgcggtg atgtggatca ttcgggtccg gcgccgtcct tcgcgatcga
     3301 agcgacactc gatgcccacc ttgcgtagga aggtcgccgc ccgacgcagc ccgcctgcca
     3361 gcgcccgagg gctattcggc caagtcttgg acttggctat tcgctggccc gccatgtctt
     3421 caaggtcgtc cagaaggacc gaggcggtcc ccgtccacac cgtccgcgcc atcatgaagg
     3481 cgcgcacagc cgcggcgatc gggtcagcgt cgatgacccg gtccacggcc tcgtcgctgt
     3541 tgccgcaata agccgaccaa aaggtgcctg ggggccacac tgccgtctcg caggcggtcg
     3601 cccacagcgc aaagtcggcc atccgtggca gcttgggcaa atgggtctcg gacaaccgct
     3661 tcaaaccctc gatcaccgca tcgagcagcg cgccgagaat gcgcgggcgc tcgatctcga
     3721 aggcggccca cagttcggcc tccggccgac ggctctcatc ggggatcggt tccagcatca
     3781 ggaacaccgc acggtccgca agatcgggcc gcgtgacgat gtcctcgatg ccgtttagga
     3841 tcaccggccg ggtggcgtcg aacagtacct cgtcctgatc ggtgtataac tggcgcaccg
     3901 cgaagcctcc accagtggcg agcctgcaca gcgtgtcgga aatccacgtc tgcaatcctg
     3961 agacgttgtc gaaggcgagc acatggccgt tgctggcggc gataaacagg tcacggtcct
     4021 cgcgcggcaa ggcgcgcaac ggcgcggtgt tgggatcgag tagcgctcgc aggatcgtcg
     4081 agaaagtgga cttcgctgag ccctgctcac ccgagagcac cagtaccgga tacggaccga
     4141 gattgcgcag acacgccagt atccaggcca ccgccagcac gaagtccgtg tcagtcctga
     4201 ggttgaggaa ggagcgcagc gtctccacag acccgccagg caccggcatc ggcaaagcct
     4261 gcatccccgc agcacggcgg aagcgcactg gcgggttatc gatcacgcgc caaccagtcg
     4321 catcgatctc gaccgcccgc caatgctcgt cgcaaaggtc gagatagagc cgatcaccca
     4381 ggccacccac acgaacatgg acagcgcgct cgggcgaatc gaaatgcgcc ttagcctcga
     4441 ggacgttcag tgccgattgc agcgcgtctg aactcggtgc cccctgcgtc gcttcaaaga
     4501 agcggcgcgc tagccaccgc cggaagccct tggaacggat cgaccaggtc tcgcggtgac
     4561 cgttgatgtc gatatcggcg aaccccgtgc tgtccggagc gtggaacagt tcggcagcgt
     4621 gcgccaattc aattaagata tcggcatgcg acgggccacg cccgccgacg taacccctct
     4681 cctcatcgat ggcttggtcg agcgccgtga cccggcaacc tgcatccttc agttgccgcc
     4741 ggagcacttc gaacgtggca cggtcctttt tcttcagttc agcgagccgt tgcagtacct
     4801 caggcgtaaa ggccgcgcca ggattggccg cggtcgtttc gactacccct tctagctgtg
     4861 gtgaaagttc atggcctggc gggtcaggaa tatcctcggc agcatttcca ggttctaccg
     4921 gatcgttggt gggactcagt gcacaccttt cttgatccga gacgatgctt tccgcgctgt
     4981 tgaccccgct ccatcgtgtg tcttgcagcg cggccacgtc agccttgtgg ctctcaatga
     5041 tggggcgtag cttttcactg cgtgtgttca gagctgatgg atcgggggtt tcgtctgcca
     5101 cgctggcagt catcgcttcc tcactcacaa ccttcgcgga ggatgcgttc cgcctccccg
     5161 acaggaatga cgcggcgacc gccgatgacg acagtgtgaa ttttaccggc cttcactagc
     5221 tcgtagaatt tcgtgacgcc gataccaatg ttcttgcaga acggtttgac gcggaacgca
     5281 agcgtcggcg tgtccgtcgt cgttgacgct ctcaatgttt cacctcgctt catccgccca
     5341 cgtcacggtt cggattaggg ccaataggcg gaggggagcg aggacggacc ctccagtttt
     5401 ttttgggggg gtccgttttt agaccaaacc gagaaggctg gcttcggtga tgaaatcagc
     5461 cccggacatt tttttcagtc gcttctgtgc aaattgcaag gagcgcgtga aatcttcttc
     5521 gttcgcgaag ccggcatcca accaggcggc atcgacgaat cctaaaaatg tgtctttgcc
     5581 accagccggc ggtctgccgg tcaacagcat ccacgccaca gccagccgca gtacgaattc
     5641 gcgcttgggc cggtcacctt cattccgccc ctgcgttcga acattctcgc tgctttgttt
     5701 ggcaagggct gcacacacat cgtgagcgac ggtgagcccg aagtcggcgt ccttcatcac
     5761 ccgcacctcg tggttcacct tccagtaatt gatcagcgcc tgcagtgctt cccgtgctct
     5821 ctccgcacgc tctgcctcgc ctctccaata ttccaagtac cggtcgagcg gggtccgctc
     5881 aaacgacttg gccaccttga ttgccgcgac caatgcacgc cggccgaaac gaacggcgtc
     5941 cgataattgc cagtccggtg cgagccgtcg atttgcatgt tccgtacgga tgatttcatc
     6001 gatggcaaat tgacctgtgg ggccatcaat gctcgttggc actcgccatt tccgattagc
     6061 gcgtctcttc accggaggtc accgcccacc gccgcgacta atttggttgg cgcggggcaa
     6121 gccggacggc ggtgaagtgt ccagcgacgg ttggcctacc ggccccgcgc ctaattcatt
     6181 ctattcgaaa cccggctata cgtaagcgac acgagttgca cggcgtcgtc atcaaccagg
     6241 ccaaaacgtc ggtatttttg tcggtatctg gacttttcac actagcctat cattctgata
     6301 aatgacgact aaatttgata aagtggctgc tagtccccgc cacccaattc catgagcgat
     6361 cgcatcagca cgggaggaag cgatgagagg tcttgtcttt gcgttggcgc ttgtcggcgc
     6421 ggcagcaacg tccgcgcgcg cgcaggatgc aattcccgat ctcaagggca catggtcggg
     6481 aaagggcaag tcgatcgtgt tcggcaccaa cccccatcat cccggcgcgc aaaccgcggc
     6541 cgatccgccg cgcgtgcgcg acatcgaggc gacttacatc gtggatggcc aagacggccg
     6601 gcttgcctgg ggtcgctctt cgtccacggc cgccaatacc caagagccgt tcgcgtgggc
     6661 gattgcgagc gacaacaaga cgatcgtcgg cgccgatatg gacggctatt ttcgcatcac
     6721 cctcatttcg ccggatagca tagaaaagtg ctacacccac aacggtacca gcccgaccca
     6781 tgcgatcgtc gccacctgcc acgtgatgaa tcgcgtgagc cggtaggatt tgttgcgcct
     6841 accgagagag cgacggcgca cgcaggctga gactgcgagc catggcgttg gggatggcgc
     6901 ataggaaggc cccggcgagg ttttcaccgg ggccttagcg ctcccgttgg ctcttggggg
     6961 gcagtggggc cttccgggaa cgccacaaga ttatcgctcg acgagcggtt tggttcccgc
     7021 cgacgcgaac tgatcgttcg cggctgccac cgtcagcgca aagggcgtcg ggcgggtttg
     7081 cgccgtgcgc cacgtccgag acggcggctt gacccgcaac gatggcccga aagataatcc
     7141 gctgcattcg cgcaggcacg ccgcctcgcc attgcgaaga tcgagcctct gcttaatcgg
     7201 cctcagtcat ggaggacccg cgatgacgcg cgccatggag ctcgagcttc tcgacagcaa
     7261 aaagaaaatg atcagcgaaa cctatctccg caacgcgtgg tatgtggccg cctggtccga
     7321 tgacctcgcc gacggtcaat tgctcagccg gacgattttg aaagagccgg tcgttctttt
     7381 ccgaaaatcg gatggacatc ttgctgcact gcaggatcgc tgtccgcaca gattcgcacc
     7441 cctgcatatg ggcaagatca tcgacggcga tcgcgttcag tgtccctatc acgggttgga
     7501 attcgactcg tccggcgctt gcgtcttgaa cccg
//
LOCUS       DATLJT010000403         1837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4388068,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000403 DATLJT010000000
VERSION     DATLJT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4388068"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VM910_09310"
     CDS             <1..276
                     /locus_tag="VM910_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI62768.1"
                     /translation="NQEARMADSEEYDHRRSALHGAMGVLNPRERRIFEARRLADEPM
                     TLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGAIAKQEAAALEAAH"
     gene            complement(417..1040)
                     /locus_tag="VM910_09315"
     CDS             complement(417..1040)
                     /locus_tag="VM910_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HVI62769.1"
                     /translation="MTLRFLAMAILAAASILSAAAQTPDLAAIAKQSGTPDIPGLKIV
                     WLAPWGDVAKAHPWKNIIVHQTEGPAGSARGGAIEQSKNPTRRGVMVWVETDGTVYWA
                     VADHLVPTHTDGADRDDNKYIDNSKTYHQVNKDTSIGVEFAGNYPDVTRPATQAQIDA
                     WRILVKVLRARYDIPLDRVYAHNWIDFKDARYCEGCTLANLARQWGE"
     gene            complement(1079..>1837)
                     /locus_tag="VM910_09320"
     CDS             complement(1079..>1837)
                     /locus_tag="VM910_09320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVI62770.1"
                     /translation="LIALIVAVYAVRSVGTMFGLDFAFSRSSNPQAIVVMVLMFLSMA
                     LNFGFLLMAMDRLRSEVADLALLDDLTGVGNRRQLVQRLTEECARSVRSSDPFALLVI
                     DLDGFKAINDTHGHAAGDACLQHFTLMAQTRLRPGDLLARTGGDEFCIVLPSSTLREG
                     VMVARRILDVCRRDAEGCAGADIPIAVSIGVAQWTADIGPYPDRLIAAADHALYAAKK
                     EGKNRYAAYDPAPPLVPDSVVLRTPDFRKQASNQ"
BASE COUNT          338 a          580 c          610 g          309 t
ORIGIN      
        1 aaccaggaag cgcggatggc cgacagcgag gaatacgatc atcgccgttc cgccttgcac
       61 ggcgcaatgg gcgtgctcaa ccctcgggaa cgccgcatct tcgaggcgcg gcggcttgcg
      121 gacgagccga tgacgctgga agatctcgca gccgagttcg gcgtgtcgcg cgaacgcgtg
      181 cgccagatcg aagtccgcgc cttcgagaag gtgcagtcgg cggtcaaggg cgcgatcgca
      241 aagcaggagg cggccgcgct cgaagcggcc cactgatcgc gtatcccgcg cagacgagat
      301 tggcggattg acacgaaaga aaaagccggc ggcaacgccg gcttttttgt tgggacgttg
      361 ctgtcgtcca ggcgacccgg ccgggacgac ggcacaaatt caggaataca ccctgcttac
      421 tctccccatt gccgcgccag gttcgccagc gtgcagcctt cgcaatagcg tgcatccttg
      481 aagtcgatcc agttgtgcgc atagacgcgg tcgagcggaa tgtcgtagcg cgcgcgcagg
      541 accttcacca gaatccgcca tgcatcgatc tgcgcctgcg tcgccggccg cgtcacgtcg
      601 ggataattgc cggcgaactc caccccgatc gaagtgtcct tgttcacctg gtgataggtc
      661 ttgctgttgt cgatgtattt gttgtcgtcg cggtcggcgc cgtcggtgtg ggtcggaacc
      721 agatggtcgg ccaccgccca ataaacggtg ccgtcggtct cgacccagac catgacgcca
      781 cggcgcgtcg gattcttcga ttgctcgatc gcgccgccgc gggccgagcc cgccgggccc
      841 tcggtctggt gcacgatgat gttcttccag ggatgcgctt tcgccacatc gccccagggc
      901 gcaagccaga cgattttcag gccgggaata tccggcgtgc ccgactgctt tgcgatagcc
      961 gcgaggtcag gcgtctgggc ggcggcggac aagatggaag cggcggcgag gatcgccatc
     1021 gcaagaaagc ggagggtcat gatgaggaaa gtcccacgaa ccctcttcta gcatggcttt
     1081 attggtttga cgcctgcttg cggaaatccg gcgttcgcag cacgacggaa tcgggcacca
     1141 gcggcggcgc cggatcatag gccgcgtagc ggttcttgcc ttccttcttg gcggcataga
     1201 gtgcgtggtc ggcggccgcg atcagccgat ccggataagg gccgatgtcc gccgtccact
     1261 gcgccacacc gatcgagacc gcgatcggga tatcggcgcc ggcgcagccc tccgcatcgc
     1321 gccggcagac gtccaggatc cgtcgcgcaa ccatgacgcc ttcgcgcagg gtcgatgagg
     1381 gcagcacgat gcagaactcg tcgccgccgg tgcgcgccag caggtcgccg ggccgaagcc
     1441 gcgtctgcgc catcagcgtg aaatgctgca ggcaggcgtc gccggccgcg tggccatggg
     1501 tgtcgttgat ggccttgaag ccgtcgagat cgatgacgag cagcgcgaac ggatcgctgc
     1561 tgcgaaccga gcgcgcgcat tcttccgtca ggcgttgtac cagttgcctg cgattgccga
     1621 cgccggtcag atcgtcgagc agcgcgagat cggcgacctc gctgcgcagc cggtccatgg
     1681 ccatcaacag gaagccgaaa ttcagcgcca tcgacaggaa catcagcacc atcacgacga
     1741 tggcctgcgg gttgctggag cgcgagaagg cgaagtccag gccgaacatc gtgccgacgg
     1801 accgcaccgc ataaaccgcg acgatcagcg cgatcag
//
LOCUS       DATLJT010000404         5417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_942097,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000404 DATLJT010000000
VERSION     DATLJT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942097"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2247
                     /locus_tag="VM910_09325"
     CDS             <1..2247
                     /locus_tag="VM910_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62771.1"
                     /translation="AGARTLLAVPMLKESELVGYIIIYRQEPEPFTEKHIELVTNFAA
                     QAVIAIENTRLLNELRESLQQQTATADVLKVISRSTFDLKAVLQTLVESAVRLCDADK
                     GTITRQIDGVFHRAESCGFSAEFMDRVRAMPVEPERGSVSGRALLGGRSVHVVDVRAD
                     PEFTFAEARELGGVRTALGVPMLREGVPIGVMALARSEVRPFTEKQIELVSTFADQAA
                     IAIENVRLFEEIQDKSRQLEEASKHKSQFLASMSHELRTPLNAIIGVTEMLLEDARDF
                     KREDELEPLDRVLRAARHLLALINDILDLSKIEAGRMELHLESFSLVPTIEDVAKTVE
                     PMAANNANRMVIDCPPDLGTIYADQTRFRQALLNLASNANKFTESGAVTIAARAQQLD
                     GREWITIAVTDTGIGMTEEQMGRLFQEFSQADASTSRKYGGTGLGLAISRHFCRLMGG
                     DVTAESKLGEGSTFTIRLPRIVESIETLPIQDQSQARAEPLHPVAEEAEEPLILVVDD
                     DATVRELVVRHLERAGFVAVAARGGQEGLRLVRELRPAAVTLDIMMPDLDGWTVLAAI
                     KGDPELADIPVVLISIVDQKNRGYALGAADYLVKPVDRAKLVATLRTICGATAGRALL
                     VDDDELVRRSVRQALEPLGWKVTEAENGRIAVDSLTAAQPDVIILDLMMPTMDGFEFM
                     DQLRRRPDWQDIPVVVITAKDLTDEDRDRLNGGVERIIQKSDRDEMLRQLGREISRCV
                     KLRAARIG"
     gene            2244..2621
                     /locus_tag="VM910_09330"
     CDS             2244..2621
                     /locus_tag="VM910_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62772.1"
                     /translation="MKILYVEDNEDNVYMLKNRLTRAGFTVIVATDGAEGVAMTASDE
                     PDLVLMDLSLPGIDGEEATRRIKADAATKRIPVIALTANAMAGDREKALAAGCDDFDT
                     KPVELERLIGKIRALTPAGRGSS"
     gene            2618..5356
                     /locus_tag="VM910_09335"
     CDS             2618..5356
                     /locus_tag="VM910_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI62773.1"
                     /translation="MSDSEAALLIVDDIEDNRFALSRRLARQGYLNVTTAADGQQALD
                     LLNSRPFDLVLLDIMMPNLNGYEVLAAMKADERLRHIPVIMISAVDEIDSVIRCIELG
                     AEDYLPKPFNPTLLRARVGACLERKRLHDQVTARTRDLTEALEQQTATSEVLRVISTS
                     PGELEPVFQTMLANAVRICEAKIGTLYLREGDGFRTVATHNAPPAYVEARTRELIRPP
                     PDATLGRLAATKQVVHTADITTIASYVERNPFVVAPVELGGYRTILGVPMLKDNELIG
                     AITINRQEVCPFTDKQIDLLKSFASQAVIAIENTRLLNGLREALEQQTATSEVLQVIS
                     SSPGELEPVFETMLANAVRICGAKFGALFLAEGEAYRAVALHNAPPAFAEVRRREPIR
                     PVNPGTALGRVAATRQPVQIADIRADPAFTSDPERFAVLELAGARTMLNVPMLKGDEL
                     IGQISIFHQEVQPFTEKQIELVQNFAAQAVIAIENTRLLNELRESLQQQTATAEVLQV
                     ISASPGALEPVFDALLKNAVQLCAGNFGNLYLREGELFRLAAWHNTPPDFVERRRSER
                     YRPGPDSPPGRMLRTRSVVHVADLLNDASYAERDPGVVAGAELAGIRTIVLVPMLKEG
                     EPIGYLSIYRQEVQAFSEKQIELLENFAAQAVIAIENTRLLNELRESLQQQTATADVL
                     KVISRSTFDLKSVLQTLVESAGRLCSADYATITRQKDGVLFFAEAYGYSSDFIEYVKA
                     MPVERGRGTATGRALLEGRVIHIADVLADPEYTWAEAQRLGGYRTVLAMPMLREEVPT
                     GVLTLTRSDVRLFTEKQIELVSTFADQAAIAIENVRLFDEIQDKSRQLQLASEHKSQF
                     VASMSHELRTPLNAIIGLTDMLVTNAARFGTEKAQEPLQRVNRAGGIPRFRHVRGH"
BASE COUNT         1047 a         1707 c         1719 g          944 t
ORIGIN      
        1 gctggtgcgc ggaccttgtt agccgttccg atgctgaaag agagcgagtt ggtcggctat
       61 atcatcatct atcggcagga gccggagccg ttcaccgaaa agcacattga actggttacg
      121 aacttcgccg cccaggccgt catcgcgatt gagaacaccc gactgctgaa tgagctgcgg
      181 gaatcgcttc agcagcagac cgccaccgcc gacgtgctca aagtcatcag ccgctcgacc
      241 ttcgacctca aagcggtgct gcagacgctg gtcgaatccg ctgttcggct ttgcgatgcc
      301 gacaagggca cgatcacccg gcagatagat ggcgtattcc atcgggctga atcctgcggc
      361 ttctccgctg aattcatgga tcgggtcaga gccatgccgg ttgaaccgga acgcggaagt
      421 gtcagcggac gcgccctgct cggaggccgc agtgttcatg tcgtagatgt tcgggccgat
      481 ccagaattta ctttcgcgga ggcccgggaa ctcggcggcg ttcgcaccgc gctcggcgtt
      541 cctatgttgc gtgaaggcgt gccgatcggt gtgatggcat tggcgcgctc cgaggtgcgg
      601 ccttttaccg agaagcaaat cgaactggtc agtacgttcg cggaccaagc cgccatcgcc
      661 atcgagaacg tgcggctgtt cgaggaaatc caggacaaga gccggcagct cgaggaagcc
      721 agcaagcaca agtcgcagtt cctcgccagc atgagccacg agctgcgcac gccgctcaac
      781 gccatcatcg gcgtcaccga gatgctgctg gaggatgccc gcgatttcaa gcgtgaggac
      841 gagcttgagc cgctcgatcg cgttttgcgc gcggcgcgcc atctgctcgc cttgatcaac
      901 gacatcctcg atttgtccaa gatcgaagcg gggcgaatgg agcttcacct cgaatcattc
      961 tcgctcgtgc ccacgatcga ggacgtagcc aagacggtcg agccgatggc ggcgaacaac
     1021 gccaatcgca tggtgatcga ttgcccgccg gatctcggca cgatctacgc tgatcagacc
     1081 cgcttccggc aggcgctgct caacctggcg agcaatgcca acaagttcac ggaaagcggc
     1141 gccgttacca tcgctgcgcg ggcgcaacag ctcgatggtc gcgagtggat caccattgcc
     1201 gtcaccgaca ccgggatcgg catgaccgag gagcagatgg ggcggctgtt ccaggagttc
     1261 tcgcaggcgg atgcctcgac cagccgcaaa tacggcggca ccggccttgg tcttgcgatc
     1321 agccggcatt tctgtcgcct gatgggcggc gacgtgaccg ccgaaagcaa gctgggcgag
     1381 ggctcgacct tcaccatccg cctgccgcgc atcgtcgaga gcatcgaaac gcttccgatc
     1441 caagaccaat cccaagcgcg tgcggagccc ttgcatccgg tcgccgagga agcggaagag
     1501 ccgctgatcc tcgtcgtgga cgacgatgcg acggtgcgcg agctcgtggt gcggcatctc
     1561 gaacgcgcgg gcttcgtggc ggtcgcggcg cgcggcggcc aagaggggct gcgcctggtg
     1621 cgggaattgc ggcccgcggc ggtgaccctc gacatcatga tgcccgatct cgacggctgg
     1681 acggtgctgg ccgcgatcaa gggcgatccg gaactggccg acattccggt ggtgctgatc
     1741 tcgatcgtcg accagaagaa ccgcggctac gcgctcggcg cggcggacta cctggtgaag
     1801 ccggtcgatc gcgccaagct ggtggcgacg ctgaggacca tctgcggcgc aaccgcagga
     1861 cgcgcccttt tggtcgacga tgacgagctg gtccgccgca gcgtacgcca ggcgctcgag
     1921 ccgctggggt ggaaggtgac cgaggcggag aacggccgca tagcggtcga ctcgctcacg
     1981 gccgcgcagc ccgacgtgat catcctcgac ctgatgatgc cgacgatgga tgggttcgag
     2041 ttcatggacc agctgcgccg acgccccgac tggcaggata tccccgtcgt ggtcatcacc
     2101 gcaaaagact tgaccgacga ggatcgcgat cgcctcaacg gcggcgtcga gcgcattatt
     2161 caaaagagcg atcgcgacga aatgctgcgt caactcggtc gcgagatcag caggtgcgtc
     2221 aaactgcggg cggcgaggat cggatgaaga ttctctacgt cgaggacaac gaggacaacg
     2281 tctacatgct gaagaaccgg ctcacgcgcg ccggcttcac cgtcatcgtg gcgaccgacg
     2341 gggcggaagg cgttgctatg accgcatcag atgagcccga cctcgtcctg atggacctga
     2401 gcctgcccgg tatcgacggc gaggaggcga cccggcgcat caaagcggat gcggccacca
     2461 agcgcatccc ggtgatcgcg ctgactgcga atgcaatggc cggcgatcgg gagaaagcgc
     2521 tcgcggcagg atgtgacgac ttcgatacca aaccggtgga gctcgagcgg ctcatcggca
     2581 agatccgggc gctgacgccg gcgggacgcg gatcatcatg agcgacagcg aagccgcgct
     2641 gctcattgtc gacgacatcg aggacaatcg attcgcgctg agccgacggc tcgcacgtca
     2701 gggctatctc aacgtcacca ccgccgccga cggccagcag gccctggacc tgctcaactc
     2761 ccgaccgttc gacctcgtgc tgctcgacat catgatgccg aacttgaacg gctacgaggt
     2821 gctggcggct atgaaggcgg acgagcgcct gcggcacatc cccgtcatca tgatttccgc
     2881 cgtcgacgag atcgacagcg tcatccgctg cattgagctt ggtgccgagg actacctgcc
     2941 caagccgttc aatccgacgt tgctgcgcgc ccgcgtcgga gcctgcctgg agcgtaaacg
     3001 gcttcacgac caggtcacgg cgcgcacgcg cgatctcacg gaggcgctgg agcagcagac
     3061 ggcgacgtcg gaggtgctgc gcgtcatctc cacctcacct ggcgagctgg agccggtctt
     3121 ccagaccatg cttgcgaatg cggtgcgcat ctgcgaggcg aagatcggca cgttgtacct
     3181 ccgcgaggga gatggcttcc gtacggttgc tactcacaat gcgccgcctg cctatgtcga
     3241 ggcgcgtacg cgcgaactga tccggccgcc cccggacgcg acgctcgggc gtctcgccgc
     3301 cacgaagcag gtggtccaca ctgccgatat cacgacgata gcgtcctatg tcgaacgcaa
     3361 tccctttgtt gtcgcccccg tggagcttgg cggttatcgg acgatactcg gggttcccat
     3421 gctcaaggac aatgagctaa tcggcgcgat caccatcaat cgccaggagg tgtgcccgtt
     3481 caccgacaag cagatcgatc tgctgaagag cttcgccagc caggccgtca tcgccatcga
     3541 gaacacgcgg ctgctcaacg ggctgcgcga agctctggag cagcagaccg cgacgtccga
     3601 ggtgttgcag gtcatcagca gctcgcccgg cgagctggag cccgtgttcg aaaccatgct
     3661 ggcaaacgcg gtgcgcatct gcggggcaaa attcggggcg ctgttcctgg ccgagggcga
     3721 ggcctaccgc gcggtggcac tgcataacgc gccgccggca ttcgccgaag tgcgccgccg
     3781 cgagccgatc aggccggtaa accctggcac ggccctgggc cgcgtggccg cgaccagaca
     3841 gccggtgcag attgcagaca ttcgcgccga tcccgctttc accagcgatc ccgaacggtt
     3901 tgcagtcctt gaacttgcag gcgcgcgcac gatgctcaac gtgccgatgc tcaaagggga
     3961 tgagttgatc ggccaaattt ccatcttcca ccaagaagta cagccgttca ccgagaagca
     4021 aattgaactt gtgcaaaact tcgccgcgca ggcagtcata gccatcgaga acacgcgcct
     4081 cctgaacgag ctgcgcgagt ccctccagca gcagaccgcc accgccgagg tgctgcaggt
     4141 gatcagcgca tcgcccgggg cactcgaacc ggtatttgat gcgttgctga agaatgcggt
     4201 acagctttgc gccggcaatt tcggcaacct ttacctccgc gaaggcgagc tgttccgtct
     4261 cgccgcttgg cataatacgc cgcctgattt cgtggagcgc cgtcgcagcg agcgttatcg
     4321 tcccggcccc gactctccgc ctggccgcat gctgcgaaca agaagtgtcg tgcacgtagc
     4381 ggaccttctg aacgacgcgt catatgccga gcgcgatccc ggcgtggtcg ccggcgccga
     4441 gctcgccggc attcgcacca tcgtcctggt gcctatgctc aaggagggtg agccgattgg
     4501 gtacctatcc atctaccgcc aggaggtgca agcgttcagc gagaagcaga ttgagctgct
     4561 ggagaacttc gccgcccaag cggtgattgc gatcgagaac acgcgcctcc tcaacgagct
     4621 gcgggaatcg cttcagcagc agaccgccac cgccgacgtg ctcaaggtca tcagccgctc
     4681 gaccttcgat ctcaagtcgg tgctgcaaac gctcgttgaa tcggctggcc ggctttgtag
     4741 cgccgactac gccacgataa ctcggcagaa ggacggggtg ctctttttcg cggaggctta
     4801 cggctactcg tccgatttca tcgaatacgt caaggctatg cccgtcgagc ggggacgtgg
     4861 gacggcgact ggacgggcct tgctggaggg tcgggtgatc catatcgccg atgtcttggc
     4921 cgacccggag tacacctggg cagaggccca gaggttaggc ggctaccgca cggtcctcgc
     4981 catgccgatg ttgcgcgagg aggtcccgac cggcgttcta acattgacgc gctccgacgt
     5041 gcggctgttc accgagaaac agatcgaact ggtttcgaca ttcgccgacc aggcggcaat
     5101 tgccatcgag aacgtgcggc tgttcgatga aatccaggac aagagccgcc agcttcaatt
     5161 ggccagcgag cacaagtcgc agtttgtcgc cagcatgagc cacgagctgc gcaccccact
     5221 caacgccatc atcgggctca cggatatgtt ggtcaccaat gcggcgcgct tcggcacgga
     5281 aaaggcgcag gagccacttc agcgcgtgaa ccgcgctggt ggtattcccc gattccgtca
     5341 tgtacgaggt cactgagcgc attgcacggt tcgcggtgga agcccggctg ccctctgtct
     5401 caggctggtc ttccttt
//
LOCUS       DATLJT010000405         4924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3009915,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000405 DATLJT010000000
VERSION     DATLJT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009915"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VM910_09340"
     CDS             complement(<1..914)
                     /locus_tag="VM910_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI62774.1"
                     /translation="MTNSFDTIIIGGGAAGCVLANRLSARSGTKVLLLEAGPDTPPGQ
                     EPADVLDTYATSYYNDAYFWPDLKVHWRRKDNSPPVHFSQGRIMGGGSSVMGMVAYRG
                     TPDDYAEWEAHGAAGWGWSDVLPYYRKLETELDFTGELHGRDGPVPIRRTKPENWAPL
                     SKAVYAFAQERQIPFIADMNADFRDGYGAVPMSNWPHKRASAAICYLDVAVRARGNLM
                     VVNGATATGLVFEGRRVVGVSARIGGKTREFRAREIILSAGGVHSPAFLMRSGVGPAA
                     HLREVGIEVRADLPGVGENLSNHAIVFVG"
     gene            complement(2175..2336)
                     /locus_tag="VM910_09345"
     CDS             complement(2175..2336)
                     /locus_tag="VM910_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62775.1"
                     /translation="MSLTAIIEVAIARGCKHYSEGGEPLATTLAVPAARGAKLQVLCT
                     ERRTTAISP"
     gene            complement(2510..3526)
                     /locus_tag="VM910_09350"
     CDS             complement(2510..3526)
                     /locus_tag="VM910_09350"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HVI62776.1"
                     /translation="MIALFCLFLALFASPFKSKGRLEAENAALRYQLIIFRRRVRGRV
                     RLTNGDRVFLIQLYRWFPSVLKAITIIRPETLVRWHRAGFRRYWRWKSRALGGRPQID
                     PDLRALIRRMSIENPLWGAPRIHGELLKLGFEVAQSSVAKYMVKRCGPPSQGWRTFLC
                     NHAPDIAAMDLFVAPTIGFDLLYVFVIIRLGRRNLVWINVTPHPTAEWIACQITEAFP
                     WKEAPRYLIRDRDCVYGAAVTHRLGAMGIRDRPIAPGSPWQNGFAERLIGSIRRECVD
                     HMVVLGEAHLRRILTKYAAYYNELRTHRSLGKDAPIHRAIQHVGRIASVPVLSGLHHH
                     YCRI"
     gene            3780..4049
                     /locus_tag="VM910_09355"
     CDS             3780..4049
                     /locus_tag="VM910_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62777.1"
                     /translation="MKQLIRTGTTFARNLALSADAGQQFLYVGADKGITVVNRKTLDV
                     VGTIQPFGILGPGHHIATDSKGNIYLAQTTAGMQKLVFKGMPATQ"
     gene            complement(4549..>4924)
                     /locus_tag="VM910_09360"
     CDS             complement(4549..>4924)
                     /locus_tag="VM910_09360"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI62778.1"
                     /translation="KGQQHGAVAMGLSGALLEEIVYDENGQIRSGSFADYLLATAAEI
                     PPIEVIALDQPTPKTPTGSKGMAEGGVMGAIGALMSAVNDALQPFGVVADRQPLTPHY
                     IRALLRSKTDPSRPLAGNVEIE"
BASE COUNT         1032 a         1513 c         1454 g          925 t
ORIGIN      
        1 ccgacgaaca cgatggcatg gttggagaga ttctccccga cgccgggcag atccgcgcgc
       61 acctcgatgc cgacctcgcg caggtgcgcg gccggcccga cgcccgaccg catcaggaac
      121 gcgggcgaat gcacgccgcc ggcggagagg atgatctccc gcgcgcggaa ttcccgcgtc
      181 ttgccgccga tccgcgcgct gacgccgacg acgcggcggc cctcgaacac caagccagtc
      241 gcggtcgcac cattgacgac catcaaattg ccgcgagcgc gcaccgccac gtcgagatag
      301 cagatcgccg ccgatgcgcg cttgtgcggc cagttgctca tgggcaccgc gccgtagccg
      361 tcgcgaaaat cggcgttcat gtcggcgatg aacgggatct ggcgctcttg cgcaaaagcg
      421 tacaccgcct tcgacagcgg cgcccagttt tccggcttgg tgcggcggat cggcaccggg
      481 ccgtcgcggc cgtgcaactc gccggtgaaa tccagctcgg tctcgagctt gcgataataa
      541 ggcagcacgt cgctccaacc ccaaccggcc gcgccgtgcg cctcccattc ggcgtaatcg
      601 tccggcgttc cgcgatacgc caccatgccc atcacggagg agccgccgcc catgatgcgc
      661 ccttgcgaaa agtgaacggg cggggaattg tccttgcggc gccaatgcac cttgaggtcc
      721 ggccagaaat aggcgtcgtt gtaatacgag gtcgcgtagg tatcgagcac gtcggccggc
      781 tcttggcccg gcggcgtgtc cggtccggct tcgagcagca gcaccttcgt ccccgatcgc
      841 gcagacagcc ggttggccag cacgcatccc gccgcgccgc cgccaatgat gatggtgtcg
      901 aaactgttgg tcatggagcc aacggcaagt cctcgctaaa taagcccgta atacggggca
      961 cttctcatag ggcgccggca gcgcgcccta tctgtgtaca cagtgtgtcc acacctgccg
     1021 acgcgattca gcgcacgggc gcccggtctc cggggcggcg cgggggcatt ctaatcgcgg
     1081 tcgagaaaat gagaacggcg gcgcacatct catctacagg gtaagacctc gccgctgaca
     1141 atggaggagg gcacccggca ccgggtgtcc catgcgcagt cgggggtcgc ccgaaaacgc
     1201 cagcggcccc agcgcgagtg gttcagactc gccggagccg ctgtgattgc ccgaaagctg
     1261 tacccttgct ggtacgccgc ggatggtacg cgctcgagcg ccaaccaggc aagatttagg
     1321 gggtcactgc cgccggcgcc ggcgccgaac ggtgacgggc tggatcctcg acggtcgaac
     1381 ccaaacttcc gccaccagtg gcgccggtca tcgtctctat cacacccctc atggcgcctg
     1441 agcgcggcca ccagggtttt acggatcgac aaccatttcc ttgtaatgct tggctcgcgt
     1501 cgaacccccg ttcgacgcac aacgatccct gtagccccgg ccgcctcatc cgagcagccg
     1561 gggctattgg actcgacagg tggggatgaa acaccgagac actcatgcct cacacaattg
     1621 atttgagaag gctgaaggcc accacagttg ctggcataga ccagtcgaga aagcggattg
     1681 acgacgcgat ccaagctaaa ttgcattact tgctgcttcg ctggatgtct cagatgacgg
     1741 cagtcgagct gcatcagacg tgggaagcgt tacgtcgaaa tgcgcctgat cgcagctctc
     1801 aaccacaatc ctcggcactt tctagcgtca caaaatatca agggtgtgag ccgcgtttcc
     1861 tatgggttag cgacgtacat cattcgtggc ggcaaccgat actttgactt caggtccatg
     1921 ggcgaactaa tcgacagggc cgacacgtgg gttgccaaca atatgtttaa ggcgctgcct
     1981 gcgcctgatc gaaactacat cgatgcgctt gctgcaattc ggaattgtgt ggtccacaga
     2041 agcgacgcgg caatgcgagc atatagaagg agcctccgcg aggtttacaa catccgttcg
     2101 gcgcctcagc caaacgaatt cctctatgcg aaggacagtc gagccaaaaa tcctgatcgg
     2161 aaccagatac gactttacgg gctgatggca gtcgtgcggc gctcggtaca gagcacctga
     2221 agctttgccc ctcgcgctgc aggcaccgcg agcgtcgtcg ccagcggttc gccgccctcg
     2281 ctgtaatgct tgcaaccgcg tgcgatcgcc acctcgataa tggcggtcag ggacattgtc
     2341 gcacccgagc acttcactga ggtatgcgga aagttcttgc gcttcgttga cgctcagtga
     2401 cgtctcatta cactgaccga tcattgaatc cccaaggcac acaggtacgg tcatgaaagc
     2461 cgatgccccg cacaccccgc ggctgcgcat tccctgtctg tatcgaaaat tagattctgc
     2521 aataatggtg atgaagtccg ctgaggacag gtacagatgc gatgcggccc acgtgttgga
     2581 tcgcgcgatg gatcggagcg tctttgccaa gagaccgatg agtcctcaac tcgttataat
     2641 aggcggcata ctttgtcaga atccggcgta agtgcgcttc gcccaagaca accatatggt
     2701 caacgcactc gcgacggatc gatccgatca atctttcggc gaagccgttc tgccacggtg
     2761 agcctggagc aatgggcctg tcacgaatgc ccatagcgcc caatcggtgc gtgacggcag
     2821 cgccatagac gcaatctcga tcacggatca ggtagcgcgg agcctccttc caagggaatg
     2881 cctcggtgat ttggcacgca atccattctg cagtcggatg aggtgtgacg ttgatccaga
     2941 caaggtttcg gcggcccagt cgaatgatga cgaagacgta gagcagatcg aagccaatgg
     3001 tgggagcaac gaataggtcc atcgcggcga tgtctggcgc gtggttacac aggaaggtgc
     3061 gccatccctg ggacggcggt ccacatcgct tgaccatgta cttcgcgacg ctggactgag
     3121 cgacctcaaa gcccagcttg agcagctcgc catggatacg tggcgcgccc caaagtggat
     3181 tctcaatgct catccgccgg atcagcgcgc gcaagtccgg gtcgatttgc ggccggcctc
     3241 caagggcgcg cgatttccaa cgccagtacc ggcgaaagcc ggcccggtgc catcgcacga
     3301 gggtctcggg gcgaatgatt gtgatggcct tgaggaccga cggaaaccat cgatacagct
     3361 ggatcaagaa cacacgatca ccgttcgtga gccggacacg gccacgcacc ctccgccgaa
     3421 atatgatcag ctgatatcgg agcgccgcgt tctctgcctc aagtcggccc ttcgacttga
     3481 acggcgaggc gaacagggcc aggaagaggc agaacagagc gatcatcgcg actgacgtta
     3541 ggcgattcgc tcacgtaatc aatacggacg gagttctcgg tacacacagg ggtgcctttg
     3601 gcaagcggcc gggcggcgag gagagctgtg caatcgtctc gccaaagacc tttcctgatg
     3661 ccgagggacc cgcgcatttc aatgtcgcgc atgctatccg cgtggccaac gatgggatgg
     3721 tgtatctggc cgatcgggaa aaccggcgcg ttcaaatgtt cactaacgat ggcaggttcg
     3781 tgaagcagct gatcagaacc ggtacgactt ttgcacgcaa tttggcgctg tccgccgacg
     3841 ctggacagca gttcctgtat gtaggcgccg acaaaggcat taccgtcgtt aatcgcaaga
     3901 cgctcgatgt cgtgggcacg atccagccgt tcggcattct gggacctggc catcacatcg
     3961 cgactgactc caaaggcaat atctatctcg cgcaaaccac cgctggaatg cagaagctgg
     4021 tattcaaagg gatgccggcg acccaatagc gggatgcccg ctcgatctgg ccgcggagca
     4081 aggggcgggc cacaagtgtc cctgcgagcc gtttcccaag agtcaaacga tctcctaaca
     4141 gcgaacctca tcgtccatca tgtcaaaaat tggtagatta acttcccgat cgaccaggca
     4201 actccacatc cgataaagaa caataccgcc cacaccaaat aatccatctg cttcgtaaaa
     4261 tcggataaac cctgtgacct tttctttaat tgccctttaa ggattgctct caagaagaga
     4321 acaatcggag ctgcagccag aatgattcct atcagcttgt acacagatgt tctttcaatc
     4381 tgaagaaggc gcgaaccacc aatgatcgat ttctcggggg ccggcaggcg ggtgactctt
     4441 tcgtgtcatt cttgctcctg gcgacgcaaa tggcaggcat cccgcggcgt tttgccagct
     4501 ctaacaaatt cgatcgcgcc gcgtttggcg tacggcctta tcgcccggtc actcgatttc
     4561 cacgtttccc gctagcgggc gggaagggtc agttttgctg cgcagcagcg ctcggatata
     4621 atgcggcgtg agcggctggc ggtcggcaac gacgccgaac ggctgcagcg catcgttgac
     4681 ggcgctcatc agcgcaccga ttgcgcccat cacaccgccc tccgccatgc ccttgctgcc
     4741 ggtcggcgtc ttgggggtgg gctggtccag agcgatcacc tcaatgggcg gaatttcggc
     4801 ggcggtggcg agcagatagt ccgcgaagct tcccgaccgg atctgcccat tctcgtcgta
     4861 gacgatctct tcgagcagcg cgccgctcag acccatggcg acggcgccgt gctgctgacc
     4921 cttc
//
LOCUS       DATLJT010000406         4404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_23196,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000406 DATLJT010000000
VERSION     DATLJT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23196"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VM910_09365"
     CDS             complement(<1..690)
                     /locus_tag="VM910_09365"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HVI62779.1"
                     /translation="MPAKPLLLLTDPTHFEVSYTINPWMRPYAWAENPVGHLAAARRS
                     FQSLTAALTAAGARLEIMPGVAGLPDMVFPANAAVVLDRLALLARFRHPERRGEEKHF
                     QEHFRVLMERAFILDEVAQISPNCFQEGAGDCIWDSTRGRFWAGYGQRSTRQAAAEVS
                     AFFGRETTALELVLPRFYHLDVCFCPLSGGEIFYYPAAFSAASLSKIREVVAPGDLIE
                     ATDEEASRFNVN"
     gene            837..1259
                     /locus_tag="VM910_09370"
     CDS             837..1259
                     /locus_tag="VM910_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI62780.1"
                     /translation="MDDMDRRLVALLRENGRLPVATLAQLLKVSRGTVQNRIERLTIN
                     GVIGGFTVRLKPEAEPQRVRAVTMVAVEGEHTAVVLKSLRGIPEVHALHTTNGRWDIV
                     VELGVESLEAFDQALQRIRLIKGISNSETSLLLSTHKI"
     gene            1352..1747
                     /locus_tag="VM910_09375"
     CDS             1352..1747
                     /locus_tag="VM910_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI62781.1"
                     /translation="MLTAGAEADIEPAFASLVQQGAGALLVVQDPVLIAYRQQITALA
                     ARYAIPAVYSQRDYVVAGGFVSYGPSFPDGYRQAGVYVAKILKGEKPADLPVVEPTKF
                     ELVINLKTAKTLGVTVPQALLVAADEVIE"
     gene            complement(1906..>4404)
                     /locus_tag="VM910_09380"
     CDS             complement(1906..>4404)
                     /locus_tag="VM910_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI62782.1"
                     /translation="SFAYDMSVQRLAEQPRVTAPFSLEAWSALDALGGAIDADLRQHD
                     VRLTMGGEPTFVSIDDYQSAEWNTTALGPIKRKCADELIRRLRNRFAPGGLLHYGQGK
                     WYPGESNPRWAFALYWRRDGVPIWHDDSLIAREAADRKASSGDARVFAEGIATRLGFS
                     TDYVQPVYEDPVDRILKEGLIAENIDPSDPKIDDPVERARILKLFGSKLGEPAGFVLP
                     LQHSTAAAGTEWMSEIWSTRRGRLFLVPGDSPLGLRLPMQSLPSLEPVEYPHVVPADP
                     FAERVPLRDPADALGKNCKARDAHTDEISPPLSPDTLVQRPRTVATGRTSSGVPVRTA
                     LTVEVRDGHLRVFMPPVETLNDYLDLVWTIEATAAELELPIDLEGHGPPPDPRLNVLK
                     VTPDPGVIEVNVQPAASWREAVSITNGLYEDAHHSRLGTNKFMLDGRHTGTGGGNHVV
                     FGGLSATDSPFLRRPDLLKSLVLYALRRPSLSYLFSGLFVGPTSQAPRIDEARQDLLY
                     ELEIAMNKVPKPGGADWVPPWLVARLFHNILVDVTGNTHRAEICIDKLFSPDSTTGRL
                     GLVELRAFEMPPDARMSLAQQLLLRALIAWFWRVPLDGKPVRWGTALHDRFMLEHFLW
                     EDFLDVLDDLRRAGYAFDPVWFEAQRQFRFPIYGAVQCGGVALELRQAIEPWHVLSED
                     VPGGAVRLVDSSVERLQVKVSGLNTDRHAVTCNGRRVPLTATGRSGEYVAGVRFKAWN
                     LATGLQPTVGLNAPLIFDIVDTWNRRSLGGCVYHVVHPGGRNYETFPVNSYEAEARRR
                     ARFQHHGHTGGVIDVPNLEYSLEYPMTLDLRRPN"
BASE COUNT          920 a         1326 c         1331 g          827 t
ORIGIN      
        1 gttgacgttg aagcgtgagg cttcctcgtc cgtcgcctcg atcaaatctc caggcgccac
       61 aacttcgcgg atcttggaga gtgacgccgc ggaaaacgcc gccggatagt aaaagatctc
      121 gccgccgctc aaggggcaga agcagacatc gagatgatag aagcgcggca agacgagttc
      181 gagtgccgtc gtctcccggc cgaagaaggc cgaaacctcc gccgccgcct gtcgcgtcga
      241 gcgttgaccg tagccggccc aaaaccgccc gcgggtcgaa tcccagatgc agtcgccggc
      301 cccttcctga aaacaatttg gggagatttg tgcgacctca tcgagaatga acgcgcgctc
      361 catcagaacg cgaaagtgtt cctgaaaatg cttttcttcg ccgcggcgtt ccgggtggcg
      421 gaaccgggcg agcagtgcaa ggcgatcgag cactaccgcc gcgttggcgg gaaacaccat
      481 atccggcaga cccgcaacgc caggcatgat ctccagccgt gccccggcag cagtgagtgc
      541 cgcggtgagc gactgaaagc tgcgccgggc cgcagccaag tgacccaccg gattttcggc
      601 ccaggcataa ggtcgcatcc atgggttgat tgtgtagctc acctcgaagt gcgtgggatc
      661 ggtcaatagc aataaaggtt tcgcaggcac ggtggctcct cccccaaaga tttccggtgc
      721 caagcgagcc cgacaatcgg tcaatagact gcgaatgaaa agtaagcatt gttgacaaaa
      781 tgcgctaata ggtgtgacga atcgtcagca caatttggca tattgtcgag gagacgatgg
      841 acgacatgga ccggcgcctt gtcgcgcttc ttcgtgaaaa cggacgttta ccggtcgcaa
      901 ccctggccca gctcctcaaa gtctcgcgcg gtacggtgca gaatcgcatc gagcggctga
      961 ccatcaacgg cgtgattggc ggtttcaccg tacggctcaa accagaagcc gaaccacaac
     1021 gcgtccgcgc cgtcaccatg gtggcagtcg aaggcgaaca cacggccgtc gtgctcaaat
     1081 cgctgcgcgg catcccggaa gttcacgcgc tacataccac caacggccgc tgggacattg
     1141 tcgtcgaact cggcgtggag agcctcgagg cattcgatca ggcgctgcaa cgcatccgac
     1201 tcatcaaggg aatttcgaat tccgaaacga gcctgctgct gtcgacacat aagatttgac
     1261 cttagtggaa cagtggacat ttcgctaagg tcggccgatg tccgctctac ctccaaaagc
     1321 cgacatgaga actcagccgc gaaaacttgt catgctgact gctggcgccg aggctgacat
     1381 cgagccagct ttcgcgtcgc ttgttcaaca aggcgcgggt gcgctccttg tcgtgcagga
     1441 tccagtcctg atcgcctatc gccagcaaat cacggcgctc gcggcgcgtt acgcgatacc
     1501 tgcggtctat tcgcaacggg actacgttgt tgccggtggt ttcgtcagct atggaccgag
     1561 ctttcctgac ggctaccgcc aggcaggcgt ctatgtcgcc aaaatactca aaggcgaaaa
     1621 gccggccgac ttgccggtcg tggagccaac caaattcgag ttggtcatca acctcaagac
     1681 cgccaagacc cttggcgtca cggtgccgca agctttgctc gtggctgctg acgaagtgat
     1741 cgaatgaggc gccgaacgtc cgctttgggt cataagcgga catgccagca ccgtgttgat
     1801 cgatgtctgc tcctcagctg gatgcctacg ccgacgcgtg atgttcttcc ttaagcccgc
     1861 gccgcggctt tctgctcgct ctcgcaatgg cggcaagcat tgtcactagt tcggacgcct
     1921 cagatcgagg gtcatcggat attccagcga atattcgagg ttcggtacat cgatgacgcc
     1981 gccggtgtgg ccatggtgct ggaagcgggc gcggcggcgg gcttctgcct cgtaggagtt
     2041 gactgggaaa gtctcgtagt tgcggccgcc cggatggaca acgtggtaca cgcagccgcc
     2101 gagcgaacgg cggttccagg tgtccacgat gtcgaatatt aggggtgcgt tgagaccgac
     2161 ggtcggttgc aggcctgtcg caagattcca agccttgaac cgaacgccgg caacatattc
     2221 gccggagcgg ccagtcgcgg tcaagggcac gcggcgcccg ttgcaagtca cagcgtgacg
     2281 atcggtgttc agccccgata ctttgacctg caaccgctcg acagaggaat caacaagacg
     2341 cacagcgccg ccgggcacgt cctcgctgag tacatgccag ggctcgatcg cctggcgcaa
     2401 ctccaacgcg acaccgccac actggacggc gccgtagatc gggaagcgga attgccgctg
     2461 tgcttcaaac cagacaggat cgaacgcgta accggcgcgt ctgagatcgt ccagtacatc
     2521 gaggaaatct tcccacagga aatgctcgag catgaagcgg tcatggagcg cggtgcccca
     2581 acgcactggc ttgccatcca gtggcactcg ccagaaccaa gcgatgagcg cgcgcagcaa
     2641 caactgctgc gctagactca tgcgtgcgtc tggcggcatt tcgaaagcac gcagctcaac
     2701 cagtcccagg cgaccggtcg tactgtccgg ggagaacagt ttatcgatgc agatctcagc
     2761 gcgatgggta ttgccggtga cgtcgaccag gatattgtgg aacaggcgag cgaccaacca
     2821 cggcggcacc caatcggcgc cgcccggttt gggtactttg ttcatcgcga tctcgagttc
     2881 gtaaagtaga tcctggcgcg cctcgtcgat gcgtggagcc tggctggtcg gcccgacgaa
     2941 caacccggaa aataaatatg atagggaagg ccgtcgcagc gcgtagagca ccaagctctt
     3001 gagcagatcg ggacgccgga ggaaaggact atcggttgcg ctcaagcccc caaatacaac
     3061 gtgattgcca ccgcctgtcc cggtgtgtct gccgtcgagc atgaatttat tggtaccgag
     3121 ccgtgagtgg tgtgcgtcct cgtaaaggcc attggtgata ctgacggcct cgcgccagga
     3181 cgctgccggt tgaacattga cctcaatgac gccaggatcg ggcgtgactt tgagcacgtt
     3241 gagccgcgga tcgggcggtg gcccgtgccc ttcgaggtcg atcggcagtt cgagttcggc
     3301 cgcggtcgcc tcgatcgtcc agactaggtc gagataatca ttgagtgttt cgacaggcgg
     3361 catgaacacg cgcagatgcc catcacgaac ctctacagtc agtgccgtgc gcaccggcac
     3421 tccggaggac gtccgaccgg tggccactgt gcgggggcgc tgcactagtg tatcaggtga
     3481 gagcggcggc gatatctcgt cggtgtgtgc gtccctcgcc ttacagttct ttcctaacgc
     3541 gtcggcggga tcgcgcagcg gtacgcgttc ggcaaacggg tcggccggca cgacatgcgg
     3601 atattcgacg ggttcaagac tgggcagcga ttgcatcggc agccgcaagc cgagtggcga
     3661 atcgccgggc accagaaaca accggccgcg gcgtgtgctc cagatctcgc tcatccattc
     3721 cgttccagcc gcggccgtcg agtgctgcaa gggcagcacg aagccggccg gctcgccgag
     3781 tttactgcca aataatttca ggatgcgcgc gcgttcgacc ggatcgtcga tctttgggtc
     3841 gctcggatcg atgttctccg cgatcagccc ctcctttagg atccggtcta caggatcctc
     3901 atagaccggc tgtacataat cggtcgagaa tcccaggcgg gtggcaattc cctcagcaaa
     3961 cactcgcgca tcgccggatg aggctttgcg atcagcggct tcgcgcgcaa tcaaactgtc
     4021 atcgtgccag atcggcacac catcgcgacg ccaatacagc gcgaacgccc agcgtggatt
     4081 cgattcgccg gggtaccatt tgccttgacc ataatgaagt aatccacccg gcgcgaaacg
     4141 attgcgcaac cgccggatca attcatcggc gcacttccgc ttgattggcc cgagtgcagt
     4201 cgtgttccat tccgccgatt gatagtcgtc gatggagacg aaggtcggct cgccgcccat
     4261 ggtcagacgc acatcgtgct gccgcagatc ggcgtcgatt gctccgccga gcgcatcgag
     4321 cgcgctccaa gcctccagcg agaacggcgc cgtcacacgt ggctgttcgg cgagacgctg
     4381 cacactcatg tcgtacgcaa aaga
//
LOCUS       DATLJT010000407         3458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_942355,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000407 DATLJT010000000
VERSION     DATLJT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942355"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VM910_09385"
     CDS             <1..223
                     /locus_tag="VM910_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62783.1"
                     /translation="EALSAGLGRVADLAERLRSSGAAPQDVAVVEEAADLLIACAAER
                     RPPGSTDEGFAACPDDPSPETAGVTWLGG"
     gene            complement(469..1290)
                     /gene="istB"
                     /locus_tag="VM910_09390"
     CDS             complement(469..1290)
                     /gene="istB"
                     /locus_tag="VM910_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI62784.1"
                     /translation="MTTTNTIDAARVELLLSELRLPGIKLIWAKLAEQSDKEGWPAAR
                     FLAALAEHEMADRGRRRIERHLAEARLPAGKTLATFDFASVPMVSKAQVMALAAGDAW
                     LEEGANILLFGPPGGGKSHLSAALGLALVENGWRVMFVRTTDLVQRLQVARRELALES
                     TIAKLDRYDLLILDDLAYVTKDQAETSVLFELIAARYERRSMLITANQPFGEWGKIFP
                     DQAMTLAAIDRLVHHATILEMNVESYRRREALDRKRGRGRPPAHATIKKTETPGD"
     gene            complement(1287..2789)
                     /gene="istA"
                     /locus_tag="VM910_09395"
     CDS             complement(1287..2789)
                     /gene="istA"
                     /locus_tag="VM910_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI62785.1"
                     /translation="MRLYMRYRQTHGTAIAGAKAGFSRATGYRIEDDPRLPSQKKAPR
                     GRRRPDPLAEVWDGEIVPILKSAPGIRAIAVLEEIRRRHPEITLGIRRTLERRIRTWR
                     ALVGPERDVIFRQEHEPGRLGLSDFTDTSALGVTVAGVALGHRLYHFRLAFSGFEHAC
                     VVLGGESFVALAEGLQNALWALGGVPHEHRSDSLSAAFRNLDRDAQEDLTRRYQGLMR
                     DYDMTPTRNNPGVAHENGSIESSHGHLKKALEDALHLRGSRDFDDLHAYRRFVDEIVG
                     RRNANNRRRIDLERPTLAALPKRRTTDYEEKTVTVTSSGGFILRRVFYTAPSRLIGHR
                     LRVHLYDDRLECFLGATPMMTLRRGRPLSERKGGHVIDYRHVIHALRKKPMALTNLVY
                     RDQLFPRPAYARAFEALHQSVGDQRACRLTVELLALAHDRGCEAELAGLIDAELDAGR
                     LPDLPTMRQRFAPNPASVPVIKVAFVPLSTYDALAAVHAIKPTSTLEAGA"
BASE COUNT          590 a         1063 c         1160 g          645 t
ORIGIN      
        1 cgaggcattg agcgccggtt tgggtcgtgt tgccgacctc gcggagcggc ttcgctcgtc
       61 cggagccgcg ccgcaggatg ttgcggtcgt ggaggaggcg gccgatctct tgatcgcctg
      121 tgcggcggag cgtcgaccac ccggctcaac cgatgaaggc ttcgctgcat gtcccgacga
      181 cccctctccc gagaccgctg gggtaacctg gctcggagga tagaggctcg gccatcacat
      241 cgtgacccgg aaaaaccgtc gggacggcct cgtcggcagc ctgacatatc ccgtctcaag
      301 tgaaagccta gcgacggcca ccgctcgcac gcggcgcggc gcgccatata gcgcgacaat
      361 ctgggtgaga acggcgcgtc aatcaggatg agacgaggcg gccgagcgat gcgatgattg
      421 acgctggcgg tcgttttggc aagcgctttg attgacgtgg aaggtcgatc agtctcctgg
      481 tgtctctgtt ttcttgattg tcgcgtgggc tggcggtcgg ccgcgaccac gctttcgatc
      541 gagggcttcg cggcggcgat agctctcgac attcatctcc aggatcgtgg cgtgatggac
      601 gaggcgatcg attgccgcga gagtcatggc ctggtccggg aagatcttgc cccattcgcc
      661 gaaaggctgg ttggccgtga tcagcatgga tctgcgttcg taacgggctg cgatcaactc
      721 gaataggacg ctggtttcgg cttgatcctt ggtgacgtag gcgaggtcgt cgaggatgag
      781 cagatcgtag cggtcgagct tggcgattgt gctttcgaga gcgagctcgc ggcgagcaac
      841 ctggagccgt tgcacgagat cggtggtgcg aacgaacatc acgcgccatc cgttctcgac
      901 caaggcaagc ccgagggctg ccgacagatg actcttgccg ccgcccggcg ggccgaagag
      961 caggatgttg gcaccttcct ccaaccaagc atcgccggcc gccagagcca tcacctgagc
     1021 tttcgagacc attggcacgg atgcgaagtc gaaggtggcg agcgtcttgc cggcgggcag
     1081 acgcgcctcg gcaagatggc gctcgatacg gcggcggcca cggtcggcca tctcgtgctc
     1141 ggcaagcgcc gccaggaagc gggccgccgg ccagccttcc ttgtcggatt gttcggcgag
     1201 cttggcccaa atcaacttga tgcccggtaa gcgcaactcg ctgagcaaga gctcgacgcg
     1261 ggcggcatcg atggtgttgg tcgtggtcat gccccggcct ccagggtgga ggttggtttg
     1321 atggcatgga cggcggcgag ggcgtcgtag gtgctgagcg gtacgaacgc caccttgatc
     1381 accggcaccg acgccgggtt tggagcgaac cgctggcgca ttgtcggcag atctggcaac
     1441 cgacccgcat cgagttccgc gtcaatcagc cctgccagct cagcctcgca gccgcgatcg
     1501 tgggcgagcg ccagcaactc gaccgtcaac cggcaggcgc gctggtcacc gacgctctgg
     1561 tgcaacgcct cgaaggctcg tgcataggcc gggcgcggga acaactgatc gcggtagacc
     1621 agattggtga gcgccatcgg cttcttgcga agcgcgtgga tcacgtgccg ataatcgatg
     1681 acgtgacccc cttttctctc ggatagcggc cgtccacgcc ggagcgtcat catcggtgtt
     1741 gcgccgagga agcactcgag gcggtcatcg tagaggtgga cgcgcagacg atggccgata
     1801 agccgtgatg gcgctgtgta gaagacgcgg cgcaggatga agccgccgct cgacgtgacg
     1861 gtgacggtct tctcttcgta atcggtggtc cggcgcttgg gtagcgcagc cagcgtcggc
     1921 cgctccagat cgatccgcct gcgattgttg gcgttgcgcc ggccaacgat ctcgtcgacg
     1981 aaacggcggt aggcgtgcag gtcgtcaaag tcacgactgc cccgcaggtg cagtgcatcc
     2041 tccagcgcct tcttgagatg gccgtgcgaa ctctcaatcg agccattctc gtgcgcgaca
     2101 cccgggttgt tcctggtcgg tgtcatgtcg taatcgcgca tgagcccctg gtaccgccgc
     2161 gtcagatctt cctgcgcatc gcggtccaag ttgcggaatg ccgccgagag actgtcgctg
     2221 cgatgctcgt gcggcacgcc gccgagagcc cacagcgcat tctgcagacc ctccgccagg
     2281 gcaacaaagc tctcgccgcc gagcacgacg caggcatgct cgaagcccga gaatgcgagc
     2341 cgaaagtggt atagccgatg tcccagagcg acgccggcga ccgtgacgcc gagtgcgctc
     2401 gtgtcggtga aatccgacag gccgaggcgg ccgggctcgt gctcctggcg gaagatcaca
     2461 tcccgctcgg gtccgaccag cgctcgccag gtgcggatgc gtcgctccag cgtgcggcga
     2521 atgccgaggg tgatctcggg atggcgccgc cggatctctt cgagcacggc aatggcccgg
     2581 atgccgggcg ccgacttcag gatcggcaca atctcgccgt cccaaacctc ggccaaaggg
     2641 tccggccgcc ggcggccacg cggcgctttc ttctgcgagg gtaggcgcgg atcatcctcg
     2701 atccggtagc cggttgccct gctgaagcct gccttggcgc cagcaatggc ggttccatgg
     2761 gtttgtcggt agcgcatgta gagcctcatc tggtggtcgg tgatgtggag gccgggcaat
     2821 gtctgatccc tcaacaatga gacgaatcag aacctcgacc cggtcgctca tgaccgccag
     2881 accgcacctg cccgagcggg caagtgcgtc cccggagatg accgtccttc gctcggggct
     2941 acgcccggct ccgtccgttc aaccccggcc cattctcatc cagattgacg ctcctctctc
     3001 atcttgattg tcgccgcgca ccgtggagcg aggaacggac ctggttcttt gggtgggaag
     3061 gcgaaggctc ggtcagcagc ggcctcggct aaatcctcgc ctcggagatg gtcaccttcc
     3121 cggtcgaggt gatcgcattc ggtccgaccg cctcacaacc ttatcgctgt ggggacaagc
     3181 ctcatctacc gtgccgcaca gatcggcaag gacgcgctgt tcctctcctc cgtcacttgc
     3241 ccgtcgtcgt cggaataact tctaggcggg catccagaga gcttggaggg agcaaatagg
     3301 gcagaagggt tgtcgtgatc ggcgtggggg tgcgtggtgc aggatcagac cccggcacca
     3361 cgatacagta gagattgttg tccagcgacg ggaagggctt gggctggggt cactgcatgc
     3421 tattgggctt gctggacctc gctttcgttc ggctcgtc
//
LOCUS       DATLJT010000408         7115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3010218,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000408 DATLJT010000000
VERSION     DATLJT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010218"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VM910_09400"
     CDS             complement(<1..224)
                     /locus_tag="VM910_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI62786.1"
                     /translation="MQTTLAPSPTANMTARDRQLLAHPPYAAANIPEPYLRHVVDYSR
                     REQPGTIIVDTDARFLYYVLPGGKAVRYGVA"
     gene            complement(495..839)
                     /locus_tag="VM910_09405"
     CDS             complement(495..839)
                     /locus_tag="VM910_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62787.1"
                     /translation="MTLCAERHALRPEAIGEIEMEQILINLIAGALGGVGAGKSSPNF
                     DLGTVGNIISGLVGGGVLGQIVTLLLPSVVAAAESGNLSVGGVISQAIAGGAGGAILT
                     ALIGAIKNRAAA"
     gene            complement(1124..1813)
                     /locus_tag="VM910_09410"
     CDS             complement(1124..1813)
                     /locus_tag="VM910_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62788.1"
                     /translation="MRHTHILPAKVRIYVAGKSRILRKSSVVIILGSSRAERRSATIV
                     ALAALAVVIAWQGLRWFSPGVPQDFEECAEQAQNTSESIARNALMVDCGTRFAGRRKA
                     DGGYTYYDFLQNRSFDISGPNPTDEERKRIDLEYVNFLDSERRDAVATEFAKRHTESL
                     LADFENVRRPPIGPPTILTPKSLPASPPNLSVRKSSPHCMIEGSLSCSWEKLTSSVKN
                     AFASTSKPGSN"
     gene            1799..2011
                     /locus_tag="VM910_09415"
     CDS             1799..2011
                     /locus_tag="VM910_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62789.1"
                     /translation="MGVAHASQWVFLSEAAASRHHCERFAQQFFCSRGISYMRPLKAI
                     ATFASVVVIACSMFAASWVGLALLGY"
     gene            complement(2114..4543)
                     /locus_tag="VM910_09420"
     CDS             complement(2114..4543)
                     /locus_tag="VM910_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVI62790.1"
                     /translation="MGRMGARLLLIVSIPAALILVSEDGRAQEESEDAHVLPTLRVTA
                     PASHPKRSPPKVTTPAPAAPETKPTVYPTTPVTGAGIAADKVPASLIVVSEKDIERTE
                     SPIITDALRQYVPGASITEVAGNPFQPNLEFRGFVASPVSGTPQGLAVYQNGVRVNEA
                     FGDTVNWDLIPTAAIQSVTLVTNNPAFGLNALGGAVDIQMKNGFLYHGAEINTMGGSY
                     GRIQSSAQWGKQVDNYAVYGALEGVHDDGFRNFSPSDIRRFYGDVGARNDGNEFHLNL
                     GLADNKFGATAAVPVELLDQFWGATYTTPQTTENRVGYLNFTGKVEATPTWTLDGIAH
                     VRVFEQNVLDGNPTGTQPCGADPTLLCFGDDTTPAFGLNGVQLANPFPAGSVLGELDR
                     TLTRSTTTGGSLQATNSDELFGHTNRFVVGASFDYSTTRFDASAELGTIGPNFVVTGS
                     GIFLGQSGSPVSIGPVALRTTNQYSGLYVLDTFDVTKAFALTAGGRFNDARIVLQDQI
                     GTSLNGSETFDRFNPIVGGTYKITPELTAYAGYSEANRTPTPLELGCADPAHPCIIAA
                     FLVSDPPLQQVISHTAEAGFRGTSELNLGTLSWKFGYFRTRNIDDILAIPSPVLQGFG
                     YFQNVGDTRRQGIEAEINLKSAAWQVNASYALVDARFLDSLLVGSNSPLADANGNVQI
                     TPGNQIPAIPRHRIKASVDYSITDALKVGGDAQYYSSQYFVGDESNQAAKLPGYAVFN
                     LRASYQIDKTFQIYARVNNVFDNRYATYGTFFDINAIPNFANGGAPFTDPRSLSPAQP
                     RAFYAGLKATF"
     gene            complement(4730..6091)
                     /locus_tag="VM910_09425"
     CDS             complement(4730..6091)
                     /locus_tag="VM910_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVI62791.1"
                     /translation="MWQNLSLRGRINLLLALGLALGLAVNIARLVSEAGPRVQAEDQS
                     VIRLARAFIETIVADLNDATDPDAKLRLIAGNLNRLRHVSITRQDDAAGGQGADRITD
                     ADDLHAPPAWFVSLVHPEKTSVSVPLTVNGSPESLVIASHPNDEIAEIWDGILTQLVV
                     GSALAVALFLVMMIVIGRALKPLEALSQAMTSIEAGAYDVRVKPEGAPELAAICAKLN
                     HLAGALSEAMEDNRRHAQRAVSLQDLERKQIARELHDEFGPYLFSLRAHASALVKLAE
                     ARNAGVDALKKHGSAILQQVNALQQFNRRVLERLRPVGLAELGLRGALEALLRLWAES
                     RPDVAIETSISRSLGETGETTELTIYRVVQEALTNVFRHAGATSVDVTIEPAEQAGAQ
                     AGRGCARVRVRDNGCGLKADHRLGLGLTGMRERILALGGSLSVASGEAGVTVEAIIPN
                     AAH"
     gene            6305..6925
                     /locus_tag="VM910_09430"
     CDS             6305..6925
                     /locus_tag="VM910_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI62792.1"
                     /translation="MRVLIVDDHPIVASGCRAVLADDPDIALMEAPDAESGERVFLAE
                     HPDVCVIDINLPTVSGYELARRILAHDAAARLIMFSMNDDPIFAARAIEVGAKGYLSK
                     SGDPCDLIEAIRQVGNGGTYLPNEMARSIAFAGPAFAQSPLSKLTSREMEILRLLSAG
                     KSLSEIAWLVHASYKTVANTSSIMRQKLGVRTSAELVRLAIEHGVA"
     gene            6912..>7115
                     /locus_tag="VM910_09435"
     CDS             6912..>7115
                     /locus_tag="VM910_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62793.1"
                     /translation="MAWLDLAAIGLDSIKPGTMMVRHPVRTGFQDMTIQTVSLNKSYG
                     GMQGVYKHASRETKTDMTFSVYVP"
BASE COUNT         1386 a         2152 c         2153 g         1424 t
ORIGIN      
        1 gccacgccat aacggacggc cttgcctccc ggcaggacgt aataaagaaa ccgggcatcg
       61 gtatcgacaa tgatcgtgcc gggctgctct ctgcgagaat agtcgacgac atgccggaga
      121 tagggctccg gaatgttcgc cgcggcataa ggcggatggg ccaacaactg ccggtccctt
      181 gcagtcatat tggcggtcgg cgacggtgca agcgttgttt gcatgcaacc gccgagcaag
      241 accccgagca accctacgag caagaacgtg agttcacgcc tgggcaccgt ctgtcctcca
      301 aagcgttacg tgcccaattt gttgctcgaa tagtgcagaa atcatggcgt cggggggatg
      361 aatggcaggc atttgccgcc aagggccgca agtggaagct ctgaaggcgc gcgcactcag
      421 gagagtcggg cagcaaaggc cggcggccgc cgggcagctt tgccaggtgc cattcgggag
      481 tcgcgggctg gttgtcaggc agcagccctg ttcttgattg caccgatcag tgccgtgagt
      541 attgcgcctc ctgcgccgcc cgcgatcgcc tgcgagatga cgccgccaac gctcagattt
      601 cccgactccg ctgcggccac gacggagggc agcaacaacg taacgatctg cccaagcact
      661 ccgccgccga ccaaaccgga gatgatattg ccaaccgtac caaggtcgaa attcggcgat
      721 gacttgcctg cgccaacgcc tccaagcgct ccggcaatga ggttaatcaa aatctgttcc
      781 atctcaattt ctcctatggc ttcggggcga agagcgtgtc gctcggcgca aagcgtcaag
      841 ccaactcact ctccgtttct cgggtgctgc atgggttggc ataagtcaca tccatcgatc
      901 gtcgtgcact ctgtcagcaa acgaatcgga cttcccataa tggaatctat caatctcgaa
      961 tttctcaagc taattgaaaa aaatcgtcag cttagccacg ttgatctatg tcaagctgtt
     1021 gcggcgcagc aaggttgagc gtttattgtt tgagcatgct taccgcgcaa acgcgtgtcg
     1081 cgtttgtctc gcgcggagac ctgtgcccat ttcggaacag gctctagttg gagcccggct
     1141 tggaagtcga tgcaaaagcg ttcttcacgc tggatgttaa cttctcccaa ctgcacgaca
     1201 acgatccttc aatcatgcag tgcggggatg atttcctgac cgacaaattt ggaggtgagg
     1261 cgggcaggct tttgggtgtc aatatggtcg gcggtccaat gggcggtcga cgcacatttt
     1321 cgaagtccgc cagcaagctc tcggtgtgtc ttttcgcaaa ttcggtcgca actgcatctc
     1381 gccgttcgga gtcaagaaaa tttacatatt ccaaatcgat gcgcttgcgt tcctcatcgg
     1441 tcggattggg accggaaata tcaaagctcc ggttttgcag gaaatcgtag taggtatagc
     1501 caccgtccgc cttccggcga ccggcaaagc gcgtcccaca atccaccatc aatgcattgc
     1561 gtgcgatact ttcggaggta ttttgcgcct gctccgcgca ttcttcaaaa tcctgaggaa
     1621 cacctggtga gaaccaacga aggccttgcc acgcgatcac gacggcaagg gcagccagcg
     1681 cgacaatggt cgcggaacgc ctttcggctc ggctgcttcc taaaataatc acaacagaag
     1741 acttcctcag gattcgcgac ttacctgcaa cgtaaatgcg aacttttgca ggcagaatat
     1801 gggtgtggcg cacgcgtcac agtgggtctt tttatccgag gctgccgcct ctcgtcatca
     1861 ttgcgaacgg tttgcccagc aatttttttg ttcaagaggg atttcttaca tgcgtccact
     1921 taaagctatt gcgacctttg catcagtcgt ggtgattgcc tgttcaatgt ttgctgcaag
     1981 ctgggttggc ctcgcgctgc tcggctattg actggaactc atttgtcgcc gctgccttgg
     2041 aaggccgcgt gtttcgtgcc tccacgccag cgaccccaag cccgggatca aaaccgtccg
     2101 gggcgccgct gcttcaaaag gttgccttca ggccggcgta gaaggcgcgc ggctgagcgg
     2161 gactgagcga gcgcggatcg gtgaacggcg cgccaccatt ggcgaaattg gggatagcgt
     2221 tgatatcgaa aaaggttcca taggtcgcgt agcgattatc gaagacattg ttgacacgcg
     2281 cgtagatttg gaacgtcttg tcgatctgat aggaggcgcg gagattgaag acggcgtagc
     2341 cgggcagctt cgccgcctgg ttggattcgt cgccgacgaa atactggctg ctgtaatact
     2401 gcgcatcgcc accgaccttg agcgcatcgg tgatcgaata gtcgacgctc gccttgattc
     2461 tgtgccgggg gatcgccggg atctgattgc cgggcgtgat ctgcacattg ccgttggcgt
     2521 cggctaaggg actgttggag ccgaccaaga gcgaatcgag gaaacgcgca tcgaccagcg
     2581 catagcttgc gttcacctgc cacgcggccg atttgaggtt gatttcggcc tcgataccct
     2641 gccgccgcgt gtccccgacg ttctggaagt agccgaaacc ttgcagcacc gggctcggaa
     2701 tcgcgaggat gtcgtcgata ttcctggtgc ggaaatagcc gaacttccag ctcagcgttc
     2761 ccagattgag ctcgctggtg ccgcgaaagc cggcttccgc ggtgtgcgag atcacctgct
     2821 ggagcggcgg atcggagaca aggaaggccg cgatgatgca gggatgcgcg ggatccgcgc
     2881 agcccagttc cagcggggtc ggcgtgcggt tggcttcgga gtagccggca taggccgtga
     2941 gctccggcgt gatcttgtag gtgccaccga cgataggatt gaagcgatcg aacgtctcgc
     3001 tcccattcag cgaggtgccg atctggtcct gcagcacgat gcgcgcatcg ttgaaccgtc
     3061 cgcccgcggt gagcgcaaag gctttcgtca cgtcgaaggt atcgagcaca taaaggccgc
     3121 tgtattgatt ggtagtgcgc agcgcgaccg gcccgatcga gaccgggctt ccggattggc
     3181 ccagaaaaat cccgctgccg gtaaccacaa aattcgggcc gatagtgccg agctcggcgc
     3241 tggcgtcgaa tcgcgtcgtg ctatagtcga agctcgcgcc gacaacaaag cgattggtgt
     3301 gaccgaacaa ctcatcgctg ttggttgcct gcagcgagcc tcccgtggtg gttgagcggg
     3361 tcagggtgcg gtcgagttcg ccgagcaccg aaccggcggg aaatggattg gcaagctgga
     3421 cgccgttcag gccgaaagcc ggggtggtgt cgtcgccgaa gcacaggagc gtcggatccg
     3481 cgccgcatgg ctgcgtgccg gtcgggtttc catccagcac gttctgctcg aagactcgca
     3541 catgcgcgat accgtcgagc gtccaggtcg gggtcgcctc gaccttcccc gtgaaattga
     3601 ggtaaccgac acggttttcc gtggtttgtg gcgtcgtgta cgtcgcaccc cagaattgat
     3661 cgagcagttc gaccggcacc gccgctgtcg caccgaactt gttgtccgcg agcccgagat
     3721 tgaggtgaaa ctcgttgccg tcgttccgcg cgccgacgtc gccatagaag cgcctgatgt
     3781 ccgatggcga gaaattgcgg aagccgtcgt catgcacgcc ctcgagcgcg ccgtacacgg
     3841 cataattgtc gacctgcttg ccccattgcg ccgaactctg gatgcggcca tacgagccgc
     3901 ccatcgtgtt gatttcggcg ccgtgataaa gaaacccgtt tttcatctgg atgtcgacgg
     3961 cgccgccgag cgcgttgagg ccgaacgcgg gattgttggt caccagtgtg accgactgaa
     4021 tcgctgcggt tgggatcaga tcccaattga cggtgtcgcc gaacgcctcg ttgacgcgca
     4081 cgccgttctg gtagaccgcg agcccctgcg gcgttccgga gaccggcgag gcgacaaagc
     4141 ctcggaattc caggttcggc tgaaacggat tgccggcgac ctcggtaatg ctcgcccccg
     4201 gcacgtattg ccgcagcgca tccgtgatga tcggagactc cgtccgctcg atatctttct
     4261 cgctgaccac gatgaggctt gctggaacct tgtcggccgc gatgcccgcg ccagtcaccg
     4321 gtgtcgtcgg gtagacagtg ggtttggtct ctggcgctgc aggagcggga gtcgtgacct
     4381 tcggcgggct gcgcttggga tgcgatgccg gcgccgtcac ccgcagggtc ggcaacacat
     4441 gcgcgtcctc cgactcctcc tgagcgcggc catcttcgct cacaaggata agagccgcag
     4501 gaatcgatac aatcaacagg agccgggcgc ccatccgtcc cattcgcctc ggacacttca
     4561 caagatgtcc atactaaacg gcggggccaa aggttccact ggcaaaaagt tgtctcaccc
     4621 gcggtgattt attcccgacg ggctcgggaa aaattcccga ccgcgcttag cgccgctcga
     4681 aatcccccct ttcaggggcc tttcacgggg aggaggccca cttcgtgcgc taatgcgcgg
     4741 cgttcggaat gatcgcctcg acggtgacgc cggcctcgcc tgaggccact gaaagcgagc
     4801 cgccgagcgc caggatccgc tcgcgcatcc ccgtcaagcc gaggccgagc ctgtggtctg
     4861 ccttgagccc gcaaccattg tcccgcacac gcacgcgcgc gcaaccgcgg ccggcttgcg
     4921 ctcccgcctg ctccgccggt tcgatagtga cgtccaccga tgtcgcaccg gcgtggcgga
     4981 acacgttggt cagggcttcc tgaaccacgc gatagatggt cagttcggtg gtttcgccgg
     5041 tctcgccgag cgaacgcgaa atgctggttt cgatggcgac atcaggacgt gactcggccc
     5101 agagacgaag cagcgcctca agcgcgccgc gcagcccaag ctcggcgagt ccgaccggcc
     5161 gcaaccgctc cagcacccgc cggttgaact gttgcaaggc attgacctgc tgcaggatgg
     5221 cgctgccatg ctttttcagt gcatcgacgc cggcgttgcg ggcttccgca agcttcacca
     5281 gcgcgctcgc atgggcgcga agcgaaaaca gatagggtcc gaactcgtca tgcagttcgc
     5341 gcgcgatctg cttgcgctcc agatcctgca gcgacaccgc ccgctgggca tggcgacggt
     5401 tgtcctccat cgcctctgaa agagcgccgg caagatggtt cagcttcgca caaatcgcgg
     5461 cgagctccgg cgcgccttca ggcttcacgc gcacatcata ggcgcctgcc tcgatgctgg
     5521 tcatcgcctg cgagagcgcc tcaagcggct tcagcgcgcg gccgatcacg atcatcatga
     5581 caaggaacag cgcaacggcg agcgccgagc cgaccacgag ctgggtcaag atgccgtccc
     5641 agatttcggc gatttcgtcg ttcggatgcg aggcgatcac gagcgactca gggctgccgt
     5701 tgacggtcag gggcacgctg accgaggttt tttccggatg caccagcgac acgaaccagg
     5761 ccggcggcgc atgcagatca tcggcgtcgg tgatgcgatc agcgccctgc cctcccgcag
     5821 cgtcgtcctg gcgcgtgata ctgacatgac gaagccggtt cagattgccc gcgatcagcc
     5881 gcaacttcgc gtcgggatcc gtcgcatcgt tcaggtccgc aacgatcgtc tcgatgaaag
     5941 cgcgcgcaag acgtatcaca ctttgatcct cggcctgaac gcggggacct gcttcgctga
     6001 ccagccgcgc aatgttgacg gcaagcccga gcgccagccc aagcgccagc aacagattga
     6061 tgcgtccccg caaggaaagg ttttgccaca tcggatgtgt ccatgaccgc cgcattcggt
     6121 tcctcgtcct gataacgttg acagtcaaac gagccgccta tctaatcgaa gcgtacagca
     6181 acatcgcgcg agatgcatga ctggacgttt gcgtgccgcg aatgtatctc cgcgctggtt
     6241 cacgagcgtt cggtctgatg cgctgcagca agcggaccga catgattgtt cgaggccgat
     6301 gctcatgcgc gtgctaatcg tcgatgatca tcccattgtc gcttcgggtt gccgtgccgt
     6361 gttggccgac gatcccgaca tcgcgctcat ggaagcgccc gacgcggaga gcggcgagcg
     6421 ggttttcctc gcggaacatc ccgatgtctg cgtgatcgac atcaacctgc cgacagtgtc
     6481 gggctatgaa ctggcgcggc gcatcctcgc gcacgatgcc gccgcgcggc tgatcatgtt
     6541 cagcatgaac gacgatccga tatttgccgc acgcgcaatc gaggtcggcg ccaagggcta
     6601 tctttcgaag tccggcgatc cctgcgacct catcgaagcg atccggcagg tcggaaatgg
     6661 cggaacctat ctgccgaacg agatggcgcg aagcattgca ttcgcaggcc ccgccttcgc
     6721 gcagtcaccg ctgtccaagc tcacctcgcg cgagatggag atcttgcggc tgctcagcgc
     6781 cggcaagagt ctgtccgaga tcgcctggct ggtgcacgct tcctacaaga ccgtcgccaa
     6841 cacctcctcg atcatgcgcc agaagcttgg tgtgcgcact tccgccgaac tcgttcgtct
     6901 cgcaatcgag catggcgtgg cttgacctcg ctgcgattgg acttgatagc atcaagcccg
     6961 gcaccatgat ggtccgccat cccgttcgca caggatttca ggacatgacg atccagaccg
     7021 tttctctcaa caagtcgtat ggcggcatgc agggcgttta caagcacgcg agccgggaga
     7081 ccaaaaccga catgacgttt tcggtctatg tgccc
//
LOCUS       DATLJT010000409         6344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3010327,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000409 DATLJT010000000
VERSION     DATLJT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010327"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..85)
                     /locus_tag="VM910_09440"
     tRNA            complement(9..85)
                     /locus_tag="VM910_09440"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Asp,seq:gtc)
     gene            304..600
                     /locus_tag="VM910_09445"
     CDS             304..600
                     /locus_tag="VM910_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVI62794.1"
                     /translation="MAKKATPTTITLKHLAAAIAEDQELSKKQAEGILNDMVTRITKH
                     LKKGERIRIVGLGILQVRKRAARMGRNPATGEAIHIKASKKVAFRAAKELKEAV"
     gene            709..1965
                     /gene="pepT"
                     /locus_tag="VM910_09450"
     CDS             709..1965
                     /gene="pepT"
                     /locus_tag="VM910_09450"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HVI62795.1"
                     /translation="MTTPAFTHTVTERFLRYVVIDTQSDPASSTYPSTGKQKDLGRLL
                     VAELQAMGIGDAHLDQHGYVYATIPANTQKKVPVICFCSHMDTSPDCSGRNVKPQLVR
                     NYRGGDITLPADPAQVIRVAEHPALKDQIGNDIITTDGTTLLGADNKAGVAEIMDAAH
                     ALVQNPQIKHGTIKILFTPDEEIGRGVDKVNLKKLGADFAYTIDGESAGTIEDETFSA
                     DGVTITIEGVSTHPGFAKGKMEHAIKIAAAIVDRLPKDTCSPETTEGKQGFLHPIGIT
                     GALEKATLGFIVRDFTEVGLKEKEGLLEGIVKDVMKGFPRSTYAIEIKEQYRNMKEVI
                     DRHPEIVDYAVEAIRRAGLKPVRSSIRGGTDGSRLSFMGLPCPNIFAGEHAFHSRLEW
                     VSVQDMERAVQTIVHLAMIWEERSNV"
     gene            complement(2005..2865)
                     /locus_tag="VM910_09455"
     CDS             complement(2005..2865)
                     /locus_tag="VM910_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI62796.1"
                     /translation="MEMPVGHDRNADQIAYWNGPGGEHWTERQPLQDILLAPISQILI
                     DSAAASPGERIIDVGCGCGSTSIALAQAVAPSGFVLGIDISAPMLARARQLAPKTLPV
                     DFVLADATVHPFDPASFDVLVSRFGVMFFAEPALSFANLRRALKPTGRLAFVCWREPR
                     ENPMLMTPLQAAYKHVPKLPQPGPEDPGPFSFASEERVRRILREAGFVDVAIVPRNLS
                     LDIAIGRGLEAAVQGAIEIGPASRALHDHPEDVRAAAKESIREALKPFVRGETVPLPA
                     AIWIVTARKQ"
     gene            complement(2938..4341)
                     /locus_tag="VM910_09460"
     CDS             complement(2938..4341)
                     /locus_tag="VM910_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI62797.1"
                     /translation="MLQFFETDAKELYRPPAPMPQEGRLGLLRLLATLRRNPLECWSE
                     EFFEEPIARVKLPFTEAVLLHDPGAIKHVLVDNVSNYRKDPIQRRILAAGLKDGLLSV
                     EGERWDIQRRTLAPLFARRTVTSFRDAMLEAADVLAARWQNVPAGVIDVAAELTLLTL
                     NVLALTIFSDGIGGDFDEFRSAMNAYFASIGRIGAFDLLGVPDFVPRPGRRHLRQTMA
                     YFENVIDQIIETRRRRLDASCDADAPQDLLTLLLRALDPSTGRPMSVAEVRSNILTFL
                     SAGHETTANTLAWSVFLLSQSPHWCARVREEADRELAGDRNRLLERLVVTRSVVEEAL
                     RLYPPIAALSRMPEEDDIVGGLSIKRHALIVIAPYVLHRHRRLWDRPDIFDPERFFGP
                     ARATIPRFAYLPFGAGPRTCIGSSFALQEAIIVLATLVHRFDMQLLPEAKVWPAQKIT
                     LRPAYGLPMRVAPRSHA"
     gene            4696..4944
                     /locus_tag="VM910_09465"
     CDS             4696..4944
                     /locus_tag="VM910_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVI62798.1"
                     /translation="MPYPRLFARQKPAGRISRVAKLFLAPKRPVVECILIDYSAGGAC
                     LQLHKYVELPNRLEVLYGTTKKRCRVVWKRGLRFGVAF"
     gene            complement(5051..5125)
                     /locus_tag="VM910_09470"
     tRNA            complement(5051..5125)
                     /locus_tag="VM910_09470"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5091..5093),aa:Val,seq:tac)
     gene            complement(5168..5572)
                     /locus_tag="VM910_09475"
     CDS             complement(5168..5572)
                     /locus_tag="VM910_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI62799.1"
                     /translation="MQIDGQCHCGYVTFEADIDPQGVSICHCTDCQTLTGSPFRVTVI
                     CSGEQVRLTGGMPKVYARTGDNGRKRFQHFCPHCGAPLFTSGEGDQTDDWGIRWGSIR
                     QRDRLKPARQIWCRSAVPWIKDIEELPGRPMD"
     gene            complement(5636..>6344)
                     /locus_tag="VM910_09480"
     CDS             complement(5636..>6344)
                     /locus_tag="VM910_09480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVI62800.1"
                     /translation="KVTEKTLEEFLGVPKFRFGEIENDDQVGIVTGLAWTDVGGELLT
                     IEGVMMPGKGKMTVTGNLRDVMKESISAAASYVRSRAITYGIEPPMFDRRDIHVHVPE
                     GATPKDGPSAGVAMATAIISVMTGIPVRHDVAMTGEITLRGRVLPIGGLKEKLLAAAR
                     GGIKTVLIPEDNAKDLTEISDAIKGGMEILPVARLDDVVARALVRKPVPIVWEEDTKV
                     PVDQDTGDEAAGGLTAH"
BASE COUNT         1346 a         1967 c         1891 g         1140 t
ORIGIN      
        1 ttgatttatg gcgcgagaga cggggctcga acccgcgacc tccggcgtga caggccggcg
       61 ctctaaccaa ctgagctact cccgcgtggc gcctaaatct agctgagacg gtgggactta
      121 aaggggccac ctttgcaagt caaggatgtt gaaaaccccc cgtcaggtgc gggtttttgc
      181 aacgtcgatg caaataatag gctatttgca aggcgaattg gcatagtcag ccaaccaccg
      241 aatcatctaa ggcctggtac gtcttaggtt cctaagccag tgttcacaac gaggagggcc
      301 aacatggcga agaaagcgac cccgacaacc atcacgctca agcaccttgc ggcggcgatt
      361 gccgaggacc aggaattgtc caagaagcag gccgaaggca ttttgaacga catggtcacg
      421 cggatcacca agcacctgaa aaagggcgag cgcatccgta ttgtcggtct cggcatcctg
      481 caggtgcgca agcgcgctgc tcgcatgggt cgcaaccccg cgaccggcga agcgatccac
      541 atcaaggctt cgaagaaggt cgccttccgc gctgccaagg agctgaagga agcggtctga
      601 caaggacgcc ttgttctgag ctcaagcgtc attccggcgc aaacgcggcc ccggaatgac
      661 gctttttgtt atactcgttt aaaatcctag ccgcggttct tgcctgtgat gacgacccca
      721 gcgttcacgc acacggtgac cgaacgcttc ctgcgttacg tggtcatcga cacccagtcc
      781 gatcccgcct cttccactta tccttcgacc gggaagcaga aagatctcgg tcgcttgctc
      841 gtcgccgagt tgcaggcgat gggaatcggc gatgcccatc tcgaccagca cggttatgtc
      901 tacgcgacca ttccggcaaa cacgcagaag aaagtgccgg tgatctgttt ttgctcccat
      961 atggacacgt cgcccgactg ctcgggcagg aacgtcaagc cgcaactcgt caggaactat
     1021 cgcggcggcg acatcaccct tcccgccgac cccgcccagg tgatccgcgt cgccgagcat
     1081 ccggccctga aggaccagat cggcaacgac atcatcacaa ccgacggcac cacgctgctc
     1141 ggtgcggaca acaaggccgg cgtagcggaa atcatggatg ccgcgcatgc cctcgttcag
     1201 aatccgcaaa tcaagcacgg caccatcaag atcctgttca ccccggatga agagatcggc
     1261 cgcggcgtgg acaaggtcaa tttgaagaaa ttgggcgccg atttcgccta cacgatcgac
     1321 ggcgaaagcg caggcacgat cgaggacgag actttttccg ccgatggcgt caccatcacc
     1381 atcgaaggcg tcagcactca cccgggcttt gccaagggca agatggagca cgcgatcaag
     1441 atcgcggctg cgatcgtcga tcgcctgccg aaagatacct gctcgccgga gaccaccgag
     1501 ggcaagcaag gcttcctgca tccgatcggc atcaccggcg cgctggaaaa agcgacgctg
     1561 ggtttcatcg tgcgcgattt caccgaggtc ggcctcaagg agaaagaggg cttgctggaa
     1621 ggcatcgtca aggacgtgat gaagggcttt ccgcgctcga cttacgcgat cgagatcaag
     1681 gaacagtacc gaaacatgaa agaggtgatc gatcgtcacc ccgaaatcgt cgattacgcg
     1741 gtcgaggcga tccgccgcgc cgggctcaag ccggtgcgca gttcgatccg cggcggcacc
     1801 gacggatcgc ggctgtcgtt catgggacta ccctgcccca atatctttgc cggcgagcac
     1861 gctttccatt cacggctgga atgggtgagc gtccaggaca tggaaagagc ggtgcagacc
     1921 atcgttcatc tggccatgat ctgggaagag cgctcaaacg tctagaacgc gatggcttag
     1981 ctcgaatggc catcgcgctt tagttcattg tttgcgcgcg gtcacgatcc agatcgcagc
     2041 cggcaagggc acggtctcgc cgcgtacaaa aggcttcagc gcttcccgga tcgactcttt
     2101 ggcggctgca cgtacgtctt cgggatgatc atgtagcgcg cggctggccg gtccaatttc
     2161 gatcgcaccc tgaaccgctg cctcgaggcc ccgaccgatc gcgatatcga gcgagagatt
     2221 gcgcggtacg atcgcaacat cgacgaagcc cgcctcacgc agaatgcgcc tcacgcgctc
     2281 ctcggaggca aacgagaatg gaccgggatc ctccggtcca ggctggggca atttcggaac
     2341 atgcttgtac gctgcttgca ggggcgtcat cagcatcggg ttctcgcgcg gttcgcgcca
     2401 gcaaacaaag gcgaggcggc ccgtgggctt cagcgcacgg cgcaaattcg caaacgaaag
     2461 ggcgggctcg gcgaaaaaca tcacgccgaa acgcgagacc aaaacgtcga agcttgcggg
     2521 atcgaaggga tggacggttg cgtccgcgag cacaaaatcg accggcagag ttttgggcgc
     2581 aagctgccgc gcgcgcgcca acatcggcgc cgaaatgtcg atgccgagca cgaaaccgct
     2641 cggagcgacg gcttgcgcca gcgcaataga ggtcgagccg cagccgcaac cgacatcaat
     2701 gatgcgttcg cccggactgg ccgccgcgct gtcgatcaag atttgcgaga tcggcgccag
     2761 caggatgtcc tgtaaaggct gccgttcagt ccaatgctcg cctccgggac cgttccaata
     2821 agcgatctga tcggcgttac gatcgtggcc aaccggcatt tccatggcga cctcatctgg
     2881 cgcgatcccg ggaaggcgcg gagtgtcgat gaaatcgccc cgctttgcaa tcgttgccta
     2941 tgcatgcgaa cgcggcgcaa ctcgcatcgg caggccatag gccgggcgca gtgtgatctt
     3001 ttgcgccggc cacactttgg cttcgggcaa aagctgcatg tcgaagcgat gcaccagggt
     3061 ggcgagcaca atgatcgctt cctgcagagc aaaggacgag ccaatgcagg tgcgcggacc
     3121 ggcaccaaac ggcaaatagg caaagcgcgg aatcgttgcc ctggccggtc cgaaaaatcg
     3181 ctcgggatcg aaaatgtccg gacggtccca caggcggcga tggcgatgca gaacgtaagg
     3241 tgcgatcacg atcagcgcgt gccgcttgat cgagaggccg ccgacaatgt cgtcctcctc
     3301 cggcattcgg ctgagcgcgg cgatcggcgg ataaagacgc agcgcttctt ccaccaccga
     3361 cctggtaacg accaggcgct cgagcaggcg gttgcgatct ccggcaagct cgcggtccgc
     3421 ctcctcccgt acccgcgcgc accagtgagg tgattgcgac agcaggaaga ccgaccaggc
     3481 gagcgtattt gccgtggttt catgaccggc cgaaagaaag gtcagaatat tcgaacgcac
     3541 ctcggcgacg ctcatcggcc ggccggtcga ggggtcgagc gcacgtagca gcagggtcaa
     3601 caggtcctgg ggagcatcgg cgtcgcagga tgcatcgagc cgacggcgtc tggtttcaat
     3661 gatctgatcg atgacatttt cgaaataggc catggtctgc cgcaaatgcc tgcgcccagg
     3721 tcttggcacg aaatcgggca cgcccagcag gtcgaacgcg ccgatccgcc cgatcgaggc
     3781 gaaataggcg ttcatggcgg agcgaaactc gtcgaaatcg cccccgatgc cgtcggagaa
     3841 aatggtcagt gccaaaacat tcaaggtcaa cagcgtcaac tctgctgcaa cgtcaatcac
     3901 gcccgcgggc acattttgcc atctcgcggc gagaacgtcg gcggcctcca gcatggcgtc
     3961 cctaaacgaa gtgaccgtcc gacgcgcgaa caacggcgca agcgtacgcc gctggatgtc
     4021 ccagcgctcc ccttccacgc tgagcaggcc gtccttgagg cccgctgcga ggatgcggcg
     4081 ctggatcgga tccttgcggt aattgctgac gttatcgacg agaacgtgtt tgatcgcgcc
     4141 gggatcatgg agcaggacag cttccgtgaa tggcagttta acccgcgcga tcggctcttc
     4201 gaaaaactcc tcgctccagc attcgagcgg gttgcgtctg agcgtggcga gcaaccgaag
     4261 aagtccgagc cgtccctcct gcggcatggg tgcgggcgga cggtacaatt cttttgcgtc
     4321 agtctcaaaa aattgcagca tttaacaaca acatgaaacg aaaaaaacca cattcgaacg
     4381 ccgctgcccc gcaaaagttc ccgcccatcg cggtcaaaac ctgaaattgt actctgcgca
     4441 ggcccaccgc agcggccaag caaggcagcc acaagccgtc ctggcgggct tggaaaggcc
     4501 tcacgcgaag cctggctcat cgcggaggaa attgtatgtc ccggcgccct ccgtgcaact
     4561 gattcgaggc taacgtttta tcagagcctc agggcaagat tgacctttca attcaaagtt
     4621 gatcaaccgg ataaaccccc tcgcagcaaa ctgcctgcac aatgggaccc ttggggttac
     4681 accgagttcg tgcgtatgcc ttatcctcgc ctctttgcgc gccaaaagcc cgcgggacga
     4741 atctccaggg ttgccaagct gtttctcgct ccgaagagac cggtcgtcga atgcattttg
     4801 atcgactact cggccggcgg cgcgtgcctg caattgcaca aatacgtcga acttcccaac
     4861 cgccttgagg tgctctacgg caccacgaaa aaacgctgcc gggtagtctg gaagcggggc
     4921 ctgcgcttcg gcgttgcgtt ttagagcatg gtccggaaaa ggggcgccgg ttttccctcg
     4981 cgacaaacgt agaacgcgtt tgcgcggaga tcatgctcaa acaataaatc tggccgaaac
     5041 tcgcgaaggc tggtgggcgg tgagaggatc gaactcccga cacccggtgt gtaaaaccga
     5101 tgctctaccg ctgagctaac cgcccttgaa cccgctcttt agcgccaccc tgccctgacg
     5161 tgcaaggtta gtccattggg cgaccgggca attcttcaat atccttgatc cacggcaccg
     5221 ccgagcggca ccagatctgc cgcgcaggct tgagacgatc gcgctggcga attgaacccc
     5281 agcgaatacc ccaatcgtcg gtctggtcgc cttcgccgct ggtgaaaagc ggcgcgccgc
     5341 agtgcgggca aaagtgctga aagcgcttgc ggccgttgtc gccggtcctg gcgtagacct
     5401 tcggcattcc tccggtcaac cggacctgct cgccagagca gatcacggtc acgcggaagg
     5461 gcgagccggt cagtgtctga caatccgtgc aatggcagat cgagacgccc tgcggatcga
     5521 tgtcggcttc aaaggtgacg tagccgcaat ggcattgccc gtcgatttgc atgggatccg
     5581 caactcccac agcgaaacgg cgcccgaagg cgccgtttca gtatcttcat ttcgctcagt
     5641 gcgcggtcag gccgccggcg gcttcatcgc cggtatcctg gtcgaccggg accttggtgt
     5701 cctcctccca gacgatcggc accggcttgc gcaccagcgc gcgcgcgaca acgtcgtcga
     5761 ggcgggcgac cggaaggatc tccatgccgc ccttgatcgc gtcggaaatc tccgtcaggt
     5821 ccttggcgtt gtcctcgggg atcaggaccg tcttgatgcc gccgcgagcg gccgcaagca
     5881 gcttctcctt caacccgccg atcggcagca ccctgccgcg cagcgtgatc tcgcccgtca
     5941 tcgccacatc gtgccggacc ggaatgccgg tcatgaccga gatgatggcg gtcgccatcg
     6001 caacacccgc tgaaggtccg tccttcggcg tcgcgccttc cggcacgtgg acgtggatgt
     6061 cgcgcctgtc gaacatcggt ggctcgatcc cataggtgat cgcgcgcgaa cggacgtagg
     6121 acgccgccgc cgagatcgat tccttcatca catcgcgcaa gttacccgtg acggtcatct
     6181 tgcccttgcc gggcatcatg acaccttcga tcgtgagcag ctcgccgccg acatcggtcc
     6241 aggcaaggcc ggtgacgatg ccgacctggt cgtcgttctc gatctcgccg aagcggaact
     6301 tcggcacgcc gaggaactcc tccagcgtct tctcggtcac cttg
//
LOCUS       DATLJT010000410         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3240563,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000410 DATLJT010000000
VERSION     DATLJT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240563"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1096
                     /locus_tag="VM910_09485"
     CDS             188..1096
                     /locus_tag="VM910_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI62801.1"
                     /translation="MEAAIVQPANGVDRSFAIGVPTIKGHAMSYGTIGVTRVTPRIGA
                     YVEGVTLAKPLSNRQVEELHQAFTEHQVLFFRDQPLDVESHKRFGRYFGELHIHPNTP
                     GPEGHPEILPIHADANSKRVSGEYWHSDVSCDEEPPLGSILYLHTVPPCGGDTLFASQ
                     TAAYDALSARMKVYLEGLTATHSGDHVYRRTNLLVGRDDKGKVFPKASHPVVRTHPVT
                     KRKALFVNGHFTTHIDGLAPEESRAILNYLCEHSTREEFQVRFRWQPHSVAFWDNRCV
                     QHLAIWDYYPQTRSGRRVTIKGDRPV"
BASE COUNT          319 a          478 c          454 g          275 t
ORIGIN      
        1 cgctgacggg ctcacccagc gacttcggca agctcatcgt ggaagagacc gagaagtggg
       61 gcaaggtggt gaaattgtcc ggcgccaagc tggactgatc gaccatcgct gagagctgct
      121 cagtgcgccg tgcgtcgtcg cgcggcagca tgaagcacgt tgagttagtg gattatatgg
      181 cgtaacgttg gaagcggcca tcgtgcaacc agcaaatggt gtcgatcggt ccttcgcgat
      241 cggcgtcccc accatcaaag gtcatgccat gagctatgga accatcggtg tcacgcgcgt
      301 tacgcctcgc atcggcgcct atgtcgaggg cgtcacactg gccaagccac ttagcaaccg
      361 ccaagttgag gagctgcatc aggctttcac ggagcaccag gtgctgtttt tccgcgatca
      421 accgctcgac gtggaatccc acaagaggtt cggccgatat ttcggtgaat tgcacatcca
      481 cccgaacacg ccggggccgg aagggcatcc cgagatcctc cccatccatg ccgatgcgaa
      541 ctccaagcgt gtcagcggcg agtactggca ttccgatgtc tcctgtgacg aggagcctcc
      601 gctcggcagc atcctctatc tccacacggt gccgccctgc ggcggcgata ccctgttcgc
      661 cagccagacg gccgcctatg acgccttgtc tgcccgtatg aaggtctatc tcgaaggatt
      721 gaccgccacg cactccggcg atcacgtcta ccgccggacc aatctgctgg tgggccgcga
      781 tgacaagggc aaagttttcc cgaaggcatc gcacccagtc gtgcgcacgc atccggtgac
      841 gaagcgcaag gcgctgttcg tcaatggcca cttcaccacc catatcgatg ggcttgcacc
      901 agaggagagc cgtgcgatcc tgaactacct gtgcgagcac agcacgcgcg aggaattcca
      961 ggtccggttc cgctggcagc cccattcggt cgccttctgg gacaaccgct gcgtccagca
     1021 tctcgcaatc tgggactatt acccacagac acgctccggc cgccgggtga cgatcaaagg
     1081 cgaccggcca gtctagccca tcttcgattt atcaactgat tgatagggaa aagtcgattt
     1141 gcgccgcgcg gcggcttggc agcacatttc ttggatcggg caggatgagt tgacgtcgac
     1201 ttggcccacg gtggtctgca gggaaccgca gccccagggc aagactcgcg gctggacccg
     1261 ggcgccgcgc gaaccaatga gcatttgacg aagtcgtcca caagctcaac ggcgagatca
     1321 acaccgcgct caccgacccg aaaatcatgg cgcagcttgc cgacctgggc agcccgacga
     1381 ccggagaagt cgaaaggatg tcaggtcgat gcggccgttc tacggcgtag cgatccgcgt
     1441 tgcagccagg tgtcgtatcc atatggtggt cgatgccgcg acgagcgctg cacccaagag
     1501 caacaaaagg tacgcgatcc acgacg
//
LOCUS       DATLJT010000411         1573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_712525,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000411 DATLJT010000000
VERSION     DATLJT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712525"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..527)
                     /locus_tag="VM910_09490"
     CDS             complement(36..527)
                     /locus_tag="VM910_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62802.1"
                     /translation="MPLETLTYAALGARLTISPKAARSLARRLRLTRSLSGDGKALVS
                     VDLAEIRHTPRPPGHREAGNVVLAAKIVALQAEIARLEATAAGHRADFERERERADRL
                     MVELLQATAETMAAKEAMARLEGLLRTDGRTGGSIDGHGVTARRPAHLAADLVAADRK
                     ALR"
     gene            complement(748..>1573)
                     /locus_tag="VM910_09495"
     CDS             complement(748..>1573)
                     /locus_tag="VM910_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI62803.1"
                     /translation="DVYKPAMRARKTDRLTLESELRRAIERHEIAILYQPIIRLEDRS
                     IAGFEALARWDHPKLGRMSPSEFITVAEEIGLIVDLGTFVMDQTARQLAAWQRAMRSR
                     EPIFASVNVSSRQLLRHDLLHDIRTVLSRSSVARGTLKLELTESLVMENPEHAAQMLT
                     RIRELGVGLSIDDFGTGHSSLAYLQRFPFDTLKIDQSFVRTTSRGTRPVILKSIIALA
                     HDLGMDVVAEGAETDSDAVELYQLGCEYAQGFAFGEPMDADAAMRLLTEERLEAAS"
BASE COUNT          259 a          508 c          527 g          279 t
ORIGIN      
        1 atcttcggca ggcagggatg agaggacatc gattgctacc tcagggcctt gcggtctgcc
       61 gccaccaggt ccgcggccag gtgcgccggt cggcgtgcag ttactccgtg gccgtctatc
      121 gagccaccgg ttcggccatc ggtccgcagg aggccttcga gccgcgccat cgcctccttg
      181 gccgccatgg tctcggcggt cgcctgcagc agctcaacca tcagccggtc ggcgcgctcg
      241 cgctcacgct cgaaatcggc ccggtgaccc gctgccgttg cttcgagccg ggcgatttcc
      301 gcctgcaatg ccacgatctt tgccgccagc acgacgttgc ctgcctcacg atggcctggt
      361 ggccgcggcg tatgccgtat ttccgcaaga tccacgctca ccaatgcttt gccgtcaccc
      421 gaaagcgagc gcgtcaaccg gagccgcctg gcaagcgaac gagcggcctt cggcgagatt
      481 gtcaggcggg cgccaagtgc tgcatatgtc agtgtttcga gaggcatcgg ctgttctcct
      541 gaacgaaccg ggcggtctgt taaccgaaaa gtaaccgtag gctgcgatgg ttaagagatc
      601 gttaacggac ggcggcgatc caccaccgga gcacgccgcg gccgggatgc gttagctcac
      661 gggttccggc tcgcgcggag cctgtcatcg ggctgcgcct cgcgcgcacc cgttggcctg
      721 gccgggacga cagcgcagtg tgggacgcta gctcgccgct tccagccgct cttcggtaag
      781 gagccgcatc gcggcatcgg catccatcgg ctcgccgaag gcaaagcctt gcgcgtattc
      841 gcagccgagc tggtagagct cgaccgcgtc ggaatcggtt tccgcgcctt cggcgacgac
      901 gtccatgccg agatcgtgcg ccagcgcaat gatcgatttc aggatcaccg gccgtgtgcc
      961 gcggctcgtg gtgcgcacga aggactggtc gatcttgagc gtgtcgaagg ggaagcgctg
     1021 cagataggcg agcgacgaat ggccggtgcc gaaatcgtcg atcgagagcc cgacgccgag
     1081 ctcgcggatt cgcgtcagca tctgtgccgc gtgttcggga ttctccatca cgagcgattc
     1141 cgtcaattcc agcttgagcg tgccgcgagc gacggaagag cgcgacagca cggtgcggat
     1201 gtcgtgcagc aggtcgtgcc gcaggagctg ccgcgaggag acgttgacgg aggcgaagat
     1261 cggctcgcgc gagcgcatcg cgcgctgcca ggccgcgagc tgccgcgcgg tctgatccat
     1321 cacgaaggtg ccgagatcga cgatcaggcc gatctcctcg gcgacggtga tgaattcgct
     1381 gggcgacatc cgcccgagtt tcgggtgatc ccagcgcgcc agcgcctcga accccgcgat
     1441 cgagcggtct tccagccgga tgatcggctg ataaaggatc gcgatttcat ggcgctcgat
     1501 ggcgcgccgc agctcgcttt cgagcgtcag ccgatcggtc tttcgcgcac gcatcgcggg
     1561 cttgtagaca tcg
//
LOCUS       DATLJT010000412         5306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4388800,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000412 DATLJT010000000
VERSION     DATLJT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4388800"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VM910_09500"
     CDS             complement(<1..513)
                     /locus_tag="VM910_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HVI62804.1"
                     /translation="MITPAEARSGKGHRDENFPVASRLIHPRHRGPILAFYEFVRTAD
                     DIADHSTLAPQEKLALLDRLEATLLGKSDDDPVGVALRSQLAARQLSPRHAQDLLAAF
                     RLDVTKLRYRDWDDLIGYCTYSAMPVGRFVLDVHGESRATWPANDALCAALQIINHLQ
                     DCGKDYRQLDR"
     gene            complement(510..1655)
                     /locus_tag="VM910_09505"
     CDS             complement(510..1655)
                     /locus_tag="VM910_09505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI62805.1"
                     /translation="MTAVASIALAVWLYLLLARGAFWRCSERDDWGAEELPAWPRVAA
                     VVPARNEADCVGDSIGSLLAQDYRGGWTVILVDDDSNDGTAEIVRRAANADARLRVVR
                     SRGLPAGWTGKLWAAKQGIEAATALPQPPDYLLLTDADIVHAPDSVSRLVAHAEAKHL
                     VLTSLMVKLHCESFAERAAIPAFIFFFQMLYPFSCVNRPQSTVAAAAGGCMLVRSDAL
                     RKAGGIEVVRAALIDDCALAKALKAHGPIWLGLTERVRSVRRYPALADIRRMVARSAY
                     AQLRYSPLLLLATMAGMALTYLVPPLVAMFGSGAARWIGVGAWALMVVAFQPTLRFYR
                     LSPLWGLALPVIALQYMLFTLDSAHQYVRGQGGSWKGRVQANVSERR"
     gene            1926..2888
                     /locus_tag="VM910_09510"
     CDS             1926..2888
                     /locus_tag="VM910_09510"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HVI62806.1"
                     /translation="MARVDPAKKKAVDTDAVFQDRLARVAQDTEALLDRLLASAPIDG
                     ERARPARLLDVMRYASLGGGKRLRPFLVVESAALFDVPRQNSLMAGAALECVHCYSLA
                     HDDLPAMDNDDLRRGRPTAHKAFDEASAILAGDGLLTFAFDILSRRETHPDADVRLEL
                     VSALARAAGLGGMVGGQMLDLAAEGRFDASAPKLGEQDVKVLQAMKTGALLRFGCVAG
                     SILGKANQSQRTALERYGSALGEAFQIADDLLDVEGDPATVGKATGKDAAAHKATLVG
                     VLGPAAARRRLENLVGEAQAALGAFGTDADVLKAAARFVATRTA"
     gene            complement(2929..3005)
                     /locus_tag="VM910_09515"
     tRNA            complement(2929..3005)
                     /locus_tag="VM910_09515"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2969..2971),aa:His,seq:gtg)
     gene            complement(3283..3783)
                     /locus_tag="VM910_09520"
     CDS             complement(3283..3783)
                     /locus_tag="VM910_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI62807.1"
                     /translation="MTEAIETTVSKSRSKAAPPAAFEMPKFELPNFEIPKMEIPAAFR
                     EMAEKSVSQAKETYEKMKSAAEEATDVLEDTYAAATKGASDYGLKVIEAARANTNAAF
                     DFATQLMTVKSLSEMVELSTAHTRKQFEALTAQSKELAAIAQKVATDSAEPVKESFGK
                     VFKKVA"
     gene            complement(4024..4401)
                     /locus_tag="VM910_09525"
     CDS             complement(4024..4401)
                     /locus_tag="VM910_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI62808.1"
                     /translation="MAANDRFDVPPEMRAFAEKSVDQARQAFDGFISAAHQAMSAFEG
                     QAETARKGAKDVTEKAMTFAERNITSAFEFAQQLVRARDVQDVLRLQADYIKRQMQVL
                     TEQAKELGESTSKAAKDAAAPKR"
     gene            4569..>5306
                     /locus_tag="VM910_09530"
     CDS             4569..>5306
                     /locus_tag="VM910_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI62809.1"
                     /translation="MRIVDIRECAIPLKSDMRNSSFDFSEMTTSVVAVITDVARDGKP
                     VIGFAFNSTGRYACGAAMRARFIPRILGADPRSLLDEAQENFDPRKILAVMMQREKSG
                     GHSERSIAIGTIEVAVWDAVAKIAAKPLHRVLAERYNGGKSATKVFCYVGGGWYAPGQ
                     TVKDLQDEMRRHLDQGYTMVKMKVGGLPLAEDVRRIEAVKKILPADAQLAVDANSKFG
                     REEALAYANALAPFRLRWFEEPCDPLDF"
BASE COUNT          964 a         1700 c         1692 g          950 t
ORIGIN      
        1 gcggtcgagc tgacggtaat cctttccgca atcctgcagg tgattgatga tttgcagcgc
       61 cgcgcaaagc gcatcgttgg cgggccaggt cgcacggctc tcgccgtgga cgtccagcac
      121 gaagcgcccg accggcatgg cggaatacgt gcaatagccg atcaggtcgt cccagtcgcg
      181 ataacgcagc ttggttacgt ccagtcggaa cgcggcgagc aaatcttgcg cgtggcgcgg
      241 cgagagctgg cgcgccgcga gctgcgagcg caaagcgacg ccgaccgggt cgtcgtcgct
      301 tttgccaagc aaggtcgctt cgagccggtc gagcagcgca agcttttcct gcggcgcaag
      361 cgtggaatga tcagcgatat cgtcggcggt acgcacgaat tcgtagaacg cgaggatggg
      421 cccacgatgg cgcgggtgaa tcagccgtga tgccacggga aaattctcgt cccggtggcc
      481 tttgcccgat cgcgcttccg cgggggtgat catcgccgct ccgacacgtt ggcctgcacc
      541 cgccccttcc agcttccgcc ctgtcctcgc acatattgat gggccgaatc gagggtgaag
      601 agcatgtact gcaaagcaat aacaggcagc gcgagacccc ataacggcga caaccggtag
      661 aatcgtaacg tcggctgaaa ggcgacgacc atcagcgccc acgctccgac cccgatccat
      721 cgtgccgcgc cgctgccgaa catagccacc aagggaggca cgagataggt cagcgccatc
      781 cctgccatcg ttgccaacag taaaagcggc gagtagcgca gctgcgcata ggcggaacgc
      841 gccaccatgc ggcggatgtc ggcaagggct gggtaacgac ggacgctgcg cacgcgctcg
      901 gtcaatccga gccagatcgg tccgtgggct ttgagcgctt tcgccagggc gcagtcgtcg
      961 atgagagcgg cgcgaacgac ctcgatgccg ccagccttcc gaagggcgtc ggagcggacc
     1021 agcatgcagc cgccggctgc cgccgcaacc gtgctctgcg gtcgattcac gcaggagaac
     1081 ggatagagca tctggaagaa gaagatgaat gccggaatgg cggcgcgctc cgcaaagctt
     1141 tcacagtgga gcttgaccat gagcgaagtc agcacgaggt gctttgcctc ggcgtgtgcg
     1201 accagccggc tgacggaatc gggcgcatgc acgatgtcgg cgtcggtgag cagcagataa
     1261 tcgggcggct gcggcagcgc cgttgctgcc tcgatgccct gcttcgccgc ccagagcttg
     1321 cctgtccatc ccgccggcaa gccgcggctt ctgaccacgc gcagtcgcgc gtcggcattc
     1381 gcagcccggc gcacgatttc ggcggtgcca tcattgctgt cgtcgtcgac caggatgacc
     1441 gtccacccac cgcggtagtc ctgcgcgagc agcgatccga tactgtcgcc gacgcagtcc
     1501 gcctcgttgc gcgcaggcac cacggctgcg acccgcggcc atgccgggag ttcctctgcc
     1561 ccccaatcgt cgcgttcgct gcagcgccaa aaggctccgc gggcgagcag caggtagagc
     1621 cagaccgcaa gagcaatcga agcaacagct gtcacccggg ctaagcctcc gcgctacccc
     1681 ggcatcgccg ccgagcccaa ggccccgccg accgggtcgg taaaaacaat tcaacatatt
     1741 caatgaattg ggctaggaaa agttggacgg gcgggccgct cttgatagat tgtcaggcgg
     1801 cgcgccttcg ggctgagtgg gtaccggtcg gcggtccgga aagtgcgcta acttaacggg
     1861 ttagcgcgcg gtcggacgca aggtcggggg tatgtcttgc tgtacgcgct ctgggggcag
     1921 aacgcatggc ccgagtcgat cctgcgaaaa aaaaagccgt tgacaccgat gccgtcttcc
     1981 aggaccggct cgctcgggtt gcccaggaca cggaagcgct gctcgatcgc ctgctcgcgt
     2041 cggccccgat cgatggcgaa cgagcgcggc cggcacggct ccttgatgtg atgcgctatg
     2101 cgagcctcgg cggcggcaag cgcttgcgac cgttcctggt ggtggaatcc gccgccctgt
     2161 tcgacgtacc gcgacaaaat tcgctgatgg cgggcgcggc gttggaatgc gtgcattgct
     2221 attcgctcgc tcacgacgac cttcccgcca tggacaatga cgacctgcgg cgcggccgcc
     2281 cgaccgcgca caaggcattt gacgaggcga gcgccatcct ggcaggcgac ggccttctca
     2341 ccttcgcctt cgacattctt tcgcgtcgcg agacgcatcc cgacgccgac gtccggctcg
     2401 agctcgtctc ggcgctcgcg cgcgctgccg gcctcggcgg gatggtgggc gggcaaatgc
     2461 tcgacctcgc ggccgaagga cggttcgatg cttctgcgcc caagctcggc gagcaagatg
     2521 tcaaggtctt acaggcaatg aaaaccggag cgctcctgcg cttcggatgt gtggccggat
     2581 cgatcctggg caaggccaac caaagtcagc ggaccgcgct tgagcgttac ggttccgcgc
     2641 ttggcgaggc gtttcagatc gcggacgacc tgctcgacgt cgaaggcgat ccggcgacgg
     2701 tcggcaaagc gacgggcaag gacgcggccg cccataaggc aacgctggtc ggcgtgctcg
     2761 gacccgcggc cgcgcggcgg cggctcgaaa acctggtcgg cgaggcgcag gccgcgctgg
     2821 gcgccttcgg caccgatgca gacgtactca aagccgcggc gcgcttcgtt gccacacgca
     2881 cggcttgaag attgggcata tggcggcgga agaccgacac gacgcatttg gtacagctgg
     2941 gtggactcga accaccgacc tcttggtcca cagccaagcg ctctaaccaa ctgagctaca
     3001 gctgcacgca tcgtcgaaag tggccgcaac ctaggagcgc cgttctgctt tgacaagagc
     3061 cgaaacccgc tgcgcgagag cggggatcag gctttgcacg tgggcgctcc ggcgcgcttt
     3121 gtgggcgttg accgcgaccc actgagcgct gaaagccccg ccgcccgcaa cggttcctta
     3181 gtcgcaatat ctcgccgccg cgcggaacag caaaaagccc gggcggtgag cccgggcctc
     3241 gattgctgga gccgcccggc ttgtgccggc gagcggcgaa actcaggcga ccttcttgaa
     3301 caccttgccg aagctttcct tgaccggctc ggcggagtcg gtggccactt tctgcgcgat
     3361 cgcggccagc tccttggatt gagcggtcag ggcctcgaat tgcttgcgcg tatgcgcggt
     3421 cgaaagctcc accatttcgg acagcgactt caccgtcatc agttgggtgg cgaaatcgaa
     3481 tgccgcgttg gtgttcgcgc gcgccgcctc gatcaccttg aggccgtagt cggaggcgcc
     3541 tttggtcgca gccgcatagg tgtcctcgag cacgtccgtc gcctcttcgg cggccgactt
     3601 catcttttcg taggtttcct ttgcctgcga gacgctcttc tcggccattt cgcggaacgc
     3661 ggccgggatt tccatcttgg gtatctcaaa attcgggagt tcgaatttcg gcatttcgaa
     3721 cgcggccggc ggggccgcct tggatcggga cttggatacg gtcgtttcga tggcctcggt
     3781 catagattga tccttcggag ggcgattgat ccggcatcac acggatccgc gcccacagcc
     3841 gatccatgct tgaggtgaca acaggcccgt ggccgtcgtt ctgacatcgg aaacgccaca
     3901 gccgtctgat gggaaggata tactcctgcc tttggtgcaa tgcaacagaa atattgcgcc
     3961 gcacaatcaa gtgccgatcg gatcagcctt tttccggtcc gactgcctcg aacacgccaa
     4021 ccgtcagcgt ttgggcgcag ccgcgtcctt tgccgccttg ctcgtgctct cgccgagctc
     4081 cttcgcctgc tcggtcagca cctgcatctg gcgcttgata tagtccgcct gcagtcgcaa
     4141 cacgtcctgg acgtctctcg cccgcaccag ctgctgggca aattcgaacg cgctcgtgat
     4201 gtttcgctcg gcgaaagtca tcgccttttc ggttacgtcc ttggcgccct tgcgggcggt
     4261 ctcggcctgc ccttcgaagg cgctcatggc ttggtgcgcc gcggaaataa agccgtcgaa
     4321 ggcctgccgc gcctggtcga cgctcttttc ggcaaacgcc cgcatttccg ggggaacatc
     4381 gaaacgatcg ttggcggcca tactcatctc ctctgtggag tgggttgcta cgccggcgag
     4441 gcgcgcaacc tgctgaaata ccagcgtccc aaccgtccct cggcgcctgc gcgaacgaag
     4501 gtgtgcggaa caggaccttc gaccttgcgg accgcactgt aacaggaatc aacaggaata
     4561 aagcccagat gcgtatcgtc gacatccgcg aatgcgcgat cccgctcaag tcggacatga
     4621 ggaattcgag cttcgatttt tccgagatga cgacctcggt cgtggcggtc atcaccgatg
     4681 tcgcgcgcga cggcaagccg gtcatcggtt tcgccttcaa ctcgaccggc cgctatgcct
     4741 gcggtgcggc gatgcgcgca cgcttcatcc ctcgcatcct cggcgccgat ccgcgatcgc
     4801 tccttgacga ggcacaagag aatttcgatc cgagaaaaat tctcgccgtc atgatgcaac
     4861 gggaaaaatc cggcggtcac agcgagcgct cgatcgcgat cggcacgatc gaggtcgcgg
     4921 tctgggatgc ggttgccaag atcgcggcaa aaccgctcca ccgcgtgctg gccgagcgct
     4981 acaacggcgg caagtccgcc accaaggtgt tctgctacgt cggcggcggc tggtatgccc
     5041 caggtcagac cgtgaaagat ctgcaggacg agatgcgccg ccatctcgat caaggttata
     5101 cgatggtgaa gatgaaggtc ggaggtctcc cgctcgccga ggacgtgcgc cgcatcgaag
     5161 ccgtcaagaa gatcctgccc gcggacgcgc agctggcggt cgatgccaac tccaagttcg
     5221 gccgcgagga agccctcgcg tatgccaacg cgctcgcgcc gtttcggcta cgctggttcg
     5281 aggagccatg cgatccgctc gatttt
//
LOCUS       DATLJT010000413         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3470246,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000413 DATLJT010000000
VERSION     DATLJT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3470246"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VM910_09535"
     CDS             <1..375
                     /locus_tag="VM910_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HVI62810.1"
                     /translation="FWILIGGAWMAFGWLVAAIVMALTIIGLPWARAAFNIAAYTLMP
                     FGSRAVRRDEVTGVSDVGTGPLGVIGNLIWLVLAGWWLALGHVITAVLLAVTIIGIPF
                     AWAHLRLAGIALWPIGKAIVPA"
     gene            complement(392..1471)
                     /locus_tag="VM910_09540"
     CDS             complement(392..1471)
                     /locus_tag="VM910_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI62811.1"
                     /translation="MRPLEFGWYLPTHGDTTAYGLAEAQVAGSPELCARVVNAAEQAG
                     FEYLLIPVGATCWEAWIAGAFMAARSTKIKPLIAARPGYINPVLMAKMISTFDQMSGG
                     RICINLIAGQNESEVEAEGIRYPKEERYALMEEEVSILKALWTSHGPVHFQGRFHTLS
                     GAQIRPRPLQQPFPKFYLGGGSPQAWEMSAKHSDVHLFWGDLPEKIAENIAAIRGMAR
                     AHGREHEIGFGMRLQIICREDERDAWEAADRLVRHATERLKQERLTLYNNSVANQRVQ
                     QLAREHGDLLLPHLWTGITKVRPGAGIAVVGNPAQCAAMLQQFIDAGCYSFCLSGYLH
                     DEEAERFGRLVRPILAQNNRGRLAA"
BASE COUNT          258 a          504 c          510 g          305 t
ORIGIN      
        1 ttctggatcc tgatcggcgg cgcctggatg gcctttggct ggctcgtcgc ggcgatcgtg
       61 atggcgctca cgatcatcgg actgccctgg gcccgcgcag ccttcaacat tgccgcctac
      121 accttgatgc cgttcggctc aagggcggtg cgccgcgacg aggtcacggg cgtaagcgat
      181 gtcggcaccg gcccgctcgg ggtgatcggc aacctgatct ggctcgtgct ggcaggctgg
      241 tggcttgcgc tcggccatgt gatcaccgca gtgctgctgg cggtcaccat catcggcatt
      301 cccttcgcct gggcgcacct gaggctcgcc ggcattgcgc tctggccgat cggcaaggcc
      361 atcgtgccgg cgtaaggagc aagcacccaa tttaggccgc gagccgcccg cgattgtttt
      421 gcgcgaggat cggtcgcacc aggcgtccga agcgttccgc ctcctcgtcg tgcagatagc
      481 cggagaggca gaaagagtag cagcccgcgt cgatgaactg ctgcaacatt gccgcgcatt
      541 gcgcggggtt gccgaccacg gcgatgccgg cgcccggccg caccttggtg atgccggtcc
      601 acagatgggg caacagcaga tcgccgtgct cgcgcgcgag ttgctgcacc cgctggttgg
      661 cgaccgaatt gttgtagagc gtcaatctct cctgcttgag ccgttcggtc gcgtgccgta
      721 ccaggcgatc ggcagcttcc caggcgtcgc gctcgtcctc gcggcagatg atctgcagcc
      781 gcatgccgaa gccgatctcg tgctcgcggc cgtgcgcgcg cgccatgccc ctgatcgcgg
      841 cgatgttttc ggcaatcttc tccggcaggt cgccccagaa cagatgcacg tcggaatgtt
      901 tggccgacat ttcccaggcc tgaggtgagc cgccgccgag atagaatttc ggaaatggct
      961 gttgcagcgg ccgtggcctg atctgcgcac ccgacagcgt gtgaaatctg ccctggaaat
     1021 gcaccggacc atgcgaggtc cacagcgctt tcaggatcga gacttcttcc tccatcagcg
     1081 catagcgctc ttccttgggg tagcggatgc cttcggcttc gacctcgctt tcgttctgcc
     1141 ccgcgatcag attgatgcaa atgcgcccgc ccgacatctg gtcgaaggtc gagatcattt
     1201 tggccatcag gaccggattg atatagccgg gccttgctgc gatcagcggc ttgatcttgg
     1261 tcgagcgggc ggccatgaag gcgcccgcga tccaggcctc ccagcaggtc gcgccgacgg
     1321 ggatcaggag atattcgaag ccggcttgtt cggccgcgtt gaccacgcgc gcgcagagtt
     1381 cgggcgagcc cgcgacctgc gcttcggcaa gcccataggc cgtggtgtcg ccatgcgtgg
     1441 gcaaatacca gccaaattcg agcggacgca tggagttctc cggaaagtgc gctttgcaga
     1501 gaaccagtct acagcctgcg gcacgtccga caaccggcca ttgccgatat cgggtcaatc
     1561 ggctaaatgg agcccgg
//
LOCUS       DATLJT010000414         1796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2321294,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000414 DATLJT010000000
VERSION     DATLJT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321294"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..579)
                     /locus_tag="VM910_09545"
     CDS             complement(229..579)
                     /locus_tag="VM910_09545"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HVI62812.1"
                     /translation="MPRRREDMVQQARERIARIRAVLNAMEYLCSGTLLRRTKVCGKP
                     SCRCAQDPSARHGPYYEWGHLKGGKLVHRVVSPEQAALLRLAIANYRQARKLLRAWEK
                     QTERLIDAEAAGER"
     gene            640..>1796
                     /locus_tag="VM910_09550"
     CDS             640..>1796
                     /locus_tag="VM910_09550"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HVI62813.1"
                     /translation="MIDPALLIERLGPLPIINHFIARMGLDDALARHVHSDQRCAVPH
                     ARALGVLLRSIIVEREPIYRQQETVQGFAAGMFGIAAEEMEHLGDDRLGRALDRLFDA
                     DRAALLTDVVLAVGQAFGLKFEEIHNDSTTVSFCGAYRSAWGRKIRGRTGPAITYGIS
                     KDHRPDLKQLLFILTTTADGGIPVAFRCSDGNTSDSRTHIDTWNTLRAVAGRTDFLYV
                     ADSKLCSRENMDHIDRAGGRFVTVLPRSRSEDEEFRKWIQTHTPDWTGVWDRPHPRHA
                     DGPRDRWFVFRAPLPSAEGWTVVWVWSTLLTLRQEARRKRNMAAASEELTSLRARLAG
                     PRTRLRRAAEIDFHVKTILEKHHVAHYIKVERTVRQDHIFKQARRGRPGPNT"
BASE COUNT          338 a          584 c          522 g          352 t
ORIGIN      
        1 accgacgacc gaggcgagcc aaacaacttc gcgcgcctca tcgaggactg atttccacat
       61 gggagcgatc ccgccgttgc ttgacgcact aaacagcgcg cgggacgttc ggttccgcac
      121 caagacacaa attgctttca agctgcagaa ttaaaggcac cctgccctac tttccgcagg
      181 tcggcgatgg aatttccgcg taattttttc tccatggtct gcagtcgctt aacgctcacc
      241 ggccgcttcg gcatcgatga gacgctcggt ttgtttctcc caggcgcgta agagtttcct
      301 cgcctgccga tagttcgcaa tggcaagtcg cagaagtgcg gcttgctcgg gcgagacgac
      361 gcggtgaacg agctttccgc cttttaggtg tccccattca tagtacgggc catggcgcgc
      421 gctcgggtcc tgagcgcatc ggcagcttgg cttgccacag actttcgtgc gccgcagcag
      481 cgttcccgag cagagatact ccatcgcatt gagaacggct cgaatgcggg cgatgcgctc
      541 gcgcgcctgc tgcaccatat cttctcgtcg acgtggcacg acgcttgacc ttcctctttc
      601 tggcgtcaat agtaacgcca gatgattcgg tcaagaacaa tgattgaccc cgccctgctg
      661 atcgaacgcc tcgggcccct ccccatcatc aatcatttca ttgcgcgcat gggactggac
      721 gatgccctcg ctcgacacgt gcactccgat caacgctgtg ccgtcccgca cgcccgagcg
      781 ctcggcgtac tgttgcgctc gatcatcgtg gagcgagagc caatctaccg ccagcaggag
      841 accgtgcaag gcttcgccgc cggtatgttc ggcatcgcgg ccgaggagat ggagcacctt
      901 ggcgatgacc gcttggggcg cgccctcgac cgcctgtttg atgccgatcg tgcggcgctg
      961 ctcaccgatg tggtcctggc ggtcggccag gctttcggct tgaagttcga ggagatccat
     1021 aacgatagca ccacggtgag cttctgcggc gcttatcgca gcgcgtgggg ccgcaagatc
     1081 cgcggccgaa cggggccggc gatcacctat ggcatctcca aggaccaccg tcccgatctc
     1141 aaacagctct tgttcattct taccacgact gccgatggcg gtatccccgt cgcgtttcgc
     1201 tgcagcgacg gcaacaccag cgattcccgc acccatatcg acacctggaa cacactgcgt
     1261 gccgtggcgg gtcgaacgga cttcctctac gtcgccgatt ccaaactgtg ttcgcgcgag
     1321 aacatggacc atatcgatcg tgccggcggc cgcttcgtta ccgtgctgcc gcgctctcgt
     1381 tccgaagacg aagagtttcg caaatggatc caaacccata cgccggactg gaccggcgtt
     1441 tgggaccgtc cccatccccg ccatgccgat ggaccgcgcg atcgctggtt cgtctttcgc
     1501 gcgccattgc cttcggcaga gggttggacg gtggtctggg tgtggagcac actactcacc
     1561 ctgcgccagg aggcaaggcg gaagaggaac atggcggcgg ccagcgaaga gctcaccagt
     1621 ttacgtgcgc ggctcgccgg accaaggacc agactgcggc gcgccgccga aatcgatttt
     1681 cacgtcaaga ccatcctcga gaagcatcac gtcgcgcact acatcaaggt cgagcgtacc
     1741 gtgcgccagg accacatctt caagcaggcg cggcggggtc gaccgggacc caacac
//
LOCUS       DATLJT010000415         4888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1631922,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000415 DATLJT010000000
VERSION     DATLJT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631922"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..328
                     /locus_tag="VM910_09555"
     CDS             149..328
                     /locus_tag="VM910_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62814.1"
                     /translation="MTKDEMIEWFKSARHQRMLWAARKIARLTGATEGGAYLTLESAV
                     TEAEIAAARNFNDRG"
     gene            complement(1080..2117)
                     /locus_tag="VM910_09560"
     CDS             complement(1080..2117)
                     /locus_tag="VM910_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HVI62815.1"
                     /translation="MSESTARLGRIAILWRGDEAARRSATADTGRFKAVFAALADVGV
                     DAEPVVYEDNVLDAVRAQLATVDGVLVWVNPIHEGRNRANLDALLREVAGRGVWVSAH
                     PDVILKMGTKEVLHRTRTMSWGSDTALYRTAEAMRVELSARLAAGPRVIKRKRGNGGQ
                     GVWKVETLASPRNRPMIRVLDATKDASEELALDEFLERCVEYFEDGCVIDQPFQPRLS
                     EGVVRCYMAGDRCAGFGYQKVKALVDAPAARAEAGPRLYTFSADPRFQRLRRLMEDEW
                     TPQLTSLLNIPRRDLPMIWDADFMLGPPGADGTDSYVLGEINVSSVFPMPDEASAEIA
                     RRVADRLGSKL"
     gene            complement(4038..4113)
                     /locus_tag="VM910_09565"
     tRNA            complement(4038..4113)
                     /locus_tag="VM910_09565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Glu,seq:ctc)
     gene            complement(4361..4699)
                     /locus_tag="VM910_09570"
     CDS             complement(4361..4699)
                     /locus_tag="VM910_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62816.1"
                     /translation="MSKLSADDEAPLDPAAARIVAKVRWLMVIAGATTLLAIAAVIGV
                     IGYRVFKAEGSAPADVTALLPKGARVIAAAVAEDRIAVTLDVGGAIEIRTFDVKTLKP
                     TGRLRFATEP"
     gene            4698..>4888
                     /locus_tag="VM910_09575"
     CDS             4698..>4888
                     /locus_tag="VM910_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HVI62817.1"
                     /translation="MALQGRSETLVIAAEEGGERLDRVLAHRLAELSRSRHKALILAG
                     RVAIDGATIRDPGHRVNAGA"
BASE COUNT          939 a         1504 c         1497 g          948 t
ORIGIN      
        1 gtcgttaggc ggacgcaact caactgacat caaggcttgg atctgctgcc ttgatgtcag
       61 ttgagttgcg tccgcctaac gacgatagct agaagtgttt cgcgacatag agcgttccaa
      121 gggcggggag aggcccaagg atcgcaagat gaccaaggac gaaatgatcg agtggttcaa
      181 atcggcgcgg catcagcgca tgttatgggc cgcccggaag atcgcgcgcc tcactggggc
      241 taccgaaggt ggcgcttatc tgacgctgga gagcgccgtg accgaggctg agattgccgc
      301 ggctcggaat ttcaatgacc gcggatgaat tcatctggag aatctcggct aatacgatca
      361 cgaaggccca acactcaaca gcaggcagca ttgcaatgga gccaaggagg accactttca
      421 caccacgatt cgcaatcact ttgctgttcg tagcggtcgt cagctatttt tggggaggat
      481 tcacggttta caagcaaatc ttcccttacc gccaaataca gaacctaaag aacaaatatg
      541 tcggtgactc gctcacatca tcgccacgct atacaatgtt tcaaacattc agcccaacat
      601 ccgacgttgt catggtcggc gattcgttaa cagccgatgc gccttggaac gaaatatttc
      661 cgaaagtcca gattgctaac agaggcatgg gtggggacac gacagacaac atcttgttgc
      721 gaatggacac tgtcctttcc gtcacccaag aaaggatgag ctcgattttt ttctggatgc
      781 gcaggcgcga tgaaatgatt gccaaaggtg cgatcaacac agcgcgatct atacccgcgt
      841 ctcgaccgat caagggctcg agcaggactt caactccgct cgacgcccaa tatgatgccg
      901 cacaagccta tttgatggcg gcgtcgcgcc gtaccaccga agccccacct cggccatgga
      961 gccggcgggg caggatctcg gagcgcgctt gcgctcggga tttcacgacg acagtaccgg
     1021 ctctctgtcg gtaccgcgat ccgcaagctc cgaccattgc tggtcgcctc tcggaccatt
     1081 cagagcttag atcccagccg atcggcaact cggcgggcga tctctgccga cgcctcgtcg
     1141 ggcatcggga acaccgaact gacgttgatc tcgcctagca cgtagctgtc ggtcccgtct
     1201 gcgccgggcg ggccgagcat gaagtccgcg tcccagatca tcggtaggtc gcgccgcggg
     1261 atgttcagca aggaggtcag ctgtggcgtc cattcgtctt ccatcaaccg gcgcaggcgc
     1321 tgaaagcggg ggtcggcact gaaggtgtat agccgcggtc cggcctcggc gcgcgcggcc
     1381 ggtgcgtcga ccagggcctt aaccttctgg tagccgaagc cggcgcagcg atcgcctgcc
     1441 atgtagcagc gcaccacgcc ttcgctcagg cgcggctgga acggctgatc gattacgcag
     1501 ccgtcttcga aatactcgac gcaccgctcg agaaactcgt ccagcgccag ctcctccgat
     1561 gcgtccttgg tggcatccag gacgcgtatc atcggtcggt tgcgtggact tgccagcgtc
     1621 tcgaccttcc agacgccctg gcccccgttg ccgcgcttcc gcttgatcac gcgcgggcca
     1681 gcggcgagcc gcgccgacaa ttcgacgcgc atcgcctcgg ccgttcgata gagggcggtg
     1741 tcgctgcccc agctcatcgt gcgggtacga tggagcacct ccttggtgcc catcttgagg
     1801 atcacatcgg gatgggcgct cacccagacg ccgcgccccg cgacctcgcg cagcagcgcg
     1861 tcgagattgg cgcggttgcg cccctcatga atcgggttga cccagacgag cacgccgtcg
     1921 actgtggcga gctgtgcgcg gacggcgtcg aggacattgt cctcgtagac caccggttct
     1981 gcatcgacgc cgacatcggc gagcgccgcg aacacggcct tgaaccggcc ggtgtctgcc
     2041 gtggcactgc ggcgcgccgc ttcatcgccc cgccatagga tagcgatgcg gccaaggcga
     2101 gctgtggact cagacatgga aaacggctcc gcattcacga tgtgcgtgtc cgcgagagcg
     2161 cgcggcgcta cccgcgcagt cgagttcgcc atcaaacctg aagacggcgc aagcactcgg
     2221 tgtttgcgtt ccgccaatgc tgctcgcacg cgccgacgac gtgagcgagt gacgacggcg
     2281 cgagttcatc atcgtgtcag gccaaatgcg attctgagcg cgtcgttgtg aggaacaccc
     2341 cacgcccttt ggcgctcata ctcgcgcgat gcggccaggc agtcagccaa tgagtgaaaa
     2401 aaagtcccga gaattcctgc gatgggtcct ggtgacgccg tgatcggatg gaccaatcgc
     2461 tgctccaggt gaggatatgt ccgtaacata tttcatgctc cgaaaaatga tgggagggag
     2521 agatttccct tccttgtgcg ctgtaaatag gagcctttat ggaccagtcg caaagcatcg
     2581 tttctatggt gggccttaga attattcatg aactctcggg tgttcggtca atccttgctg
     2641 gccggcgttc tggagttctc gggagccggg ctaacgcttg tctccgtagg agcgcccacc
     2701 ccaacatcct tgaccccggt cgcttcgaga ccctgtttgg tgatgcgaag cgggcccggc
     2761 ttcagcatcg cgtcgccagg ccaggagcga gccattggcg cggacctctt ccagcagccc
     2821 agcgcggatg agcttatcca cggccttccg aaccgcttcg cccgtaacgt ttgtcggcag
     2881 gtcaaatccg cgatcatcgc gtcgcgaagc cgtggacaga atgacgagtt gggtatcggt
     2941 gagtcttgac atcgggatcg tccattgcgt tggtgcggcg tcattgccgc caccaccgca
     3001 gccccacctc ggccacgcag ccggcggggc agggtcccga gggcgcgtcg cgcctggaac
     3061 ggtcacaata ccgctccgtt cgccgccgaa tgccagtcct ttctggataa tgttattgcg
     3121 ggttttcggc acactggata atgggatgat ccgcgttcga ataccggaat aaccaagata
     3181 ccgtccaccg gttcaatccc taccgtgact gcgaatcagc ttactcgggc tttgggcgag
     3241 gctgtcattc gaatatggag caatttgcct caagacctgc aaaatcgtct atttcaggag
     3301 gcggtgacct cccatggtga atccataaga ttgccgctcg cggtcttcct gcatgacaag
     3361 cacccgcgca cttcgggccc attaggcaac cctcgcgaaa taacagagcc tgatagcctt
     3421 gggggttagc gcgcttgccg tcgcgtgaag tctcgcgatg gccctgtccc agctagtggt
     3481 gtaatgacga gcggcttccc gttggaggcg cgcaccttgt caataaacgc tcccctcttg
     3541 gcgtacatag tgcgcgtaat ttatgcaaag ctgttcggct ggctcggcgc gagcttgtct
     3601 ggatcaacgt gaccgcatac cgaccgccga aatggattgc gcgaggctgg gcgagggcca
     3661 agggtcgaaa gggaagcatt gcgtgtcact cgagacgtcg ccgagttccg gcagcgtgcg
     3721 catcctctcc agcagtttcc cgaccattcg ttgagctcgg tgacgttgga atcttgcagc
     3781 gtatactgga agatcgtgcg cgagttgggg ccgcccacgg tatgtcctgc gccggctgca
     3841 gggacagcgt cgcgcctttt cagccacgga gcgatcctcc ggcgtcgcga atgtgtgtac
     3901 gaagaacgct attttttccg acaatggttc atgcagaaat ggggaataaa atcaagcgtg
     3961 atattaggtc agttttaggc cagcccctcc tcccaggaac ctgaccaagg taatatcctg
     4021 tttacattga tatattttgg cgctccctag gggactcgaa cccctgtttt cgccgtgaga
     4081 gggcgacgtc ctgggccgct agacgaaggg agcaagcgga gaagcttcgg aaatagcgct
     4141 gatccccgcc gcgcgcaagg agagctcgcg ggtccttcac cgtgcacata acccgacgat
     4201 ccatgctctc ctcagaagag gttctgcgta gaagatggac ccccgcgtca agcccggggg
     4261 tgactccgtg gggatgggca ggagctcaaa tcaattcatc cgagtctcgc ggccgcgcgt
     4321 ggtgtcgtcg cagctcccca tgcgccgcgc ccctcgcccc tcacggctcg gtcgcaaagc
     4381 gcagccgtcc ggtgggcttg agcgtcttga cgtcgaaggt gcggatctcg atcgcgccgc
     4441 ccacgtcgag cgtcaccgcg atgcggtcct cggccacggc cgcggcgatg acgcgcgcgc
     4501 ccttcggcag cagcgcggtg acgtccgccg gcgcgcttcc ctcggccttg aaaacgcgat
     4561 agccgatgac gccgatgacc gcggcgatcg cgaggagcgt ggtggcgccg gcgatcacca
     4621 tcagccagcg caccttcgcg acgatgcggg cggccgcggg atcgaggggc gcttcatcgt
     4681 ccgccgagag cttgctcatg gccttgcagg ggcgcagcga gacactggtg atcgccgccg
     4741 aggagggcgg cgagcggctc gaccgcgtgc tggctcaccg cctggccgag ctgtcgcgct
     4801 cgcgccacaa ggcgctgatc ctggcgggtc gcgtcgcgat cgacggcgcc acgatccgcg
     4861 atcccggcca ccgcgtcaac gccggcgc
//
LOCUS       DATLJT010000416         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1402295,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000416 DATLJT010000000
VERSION     DATLJT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402295"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VM910_09580"
     CDS             <1..190
                     /locus_tag="VM910_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI62818.1"
                     /translation="EAFQGSTRTGSVGLGLAIVAELVRAHGGEIRLVEGTIGATFRLS
                     IPDRAVDLDTRRGERARA"
     gene            453..529
                     /locus_tag="VM910_09585"
     tRNA            453..529
                     /locus_tag="VM910_09585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:487..489,aa:Arg,seq:acg)
     gene            1551..1871
                     /locus_tag="VM910_09590"
     CDS             1551..1871
                     /locus_tag="VM910_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI62819.1"
                     /translation="MKHVALSIALAFFPVTTSAAAEEAQSFPKDQIATGAELYAVNCS
                     PCHGARMQDPNAAFNLRKFPLHQHERFVNAVTRGKNQMPPWGDFFKPDQLEALWAYIM
                     AGER"
     gene            complement(2216..2626)
                     /locus_tag="VM910_09595"
     CDS             complement(2216..2626)
                     /locus_tag="VM910_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62820.1"
                     /translation="MASTMQDAPEAGGGPVTGFAHDSRMPTGNHTGPHHSPPIRTIGV
                     DLGKNSFHLVGLDQRGAIVFALAYANVAFSGAVSICMLNLLCSAVRWTGNMALPAGVI
                     VGSVIAHILISPLLIFGWGPMPALGPAGAEGSAP"
     gene            complement(2838..3014)
                     /locus_tag="VM910_09600"
     CDS             complement(2838..3014)
                     /locus_tag="VM910_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI62821.1"
                     /translation="MRNPQWAVDLRTFPRDGHARFVDAVANGKRNMPAWDDVLDPEQI
                     EALWAYVITGEAGE"
     gene            complement(3007..3168)
                     /locus_tag="VM910_09605"
     CDS             complement(3007..3168)
                     /locus_tag="VM910_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62822.1"
                     /translation="MTQPIANAFVTASLGFALLGVNAAAQERAPEFPAEQIKKGADLF
                     ARNCATEDA"
BASE COUNT          679 a         1030 c          997 g          591 t
ORIGIN      
        1 cgaagccttc cagggctcga cccgcaccgg cagcgtcggg ctcgggctcg ccatcgtggc
       61 cgagctggtg cgcgcccacg gcggcgagat ccgcctggtc gaaggcacca tcggcgcgac
      121 cttccgcctg tcgatcccgg accgcgcggt cgatctcgac acgcggcgcg gcgagcgcgc
      181 aagggcgtga gaggcgggcg tggccgccgt cattgcggga gcacgtgcac cgtcattgcc
      241 gggcatagcg cgtcggaaga cgcgcgtgga cgcgcttatg acccggcaat ccatcctctt
      301 cgcaaagact tcttaggaag aacgatggac ccgcgggtga cagaggcacg tggacaccga
      361 accttgccaa cattggccga agcctatagc tagaggcccg ttcccggcga ctttggcttc
      421 tccggggaca cttcccgcgc gatgcgcaag acgcgcccgt agctcagctg gatagagcac
      481 cagactacga atctgggggt caggagttcg aatctcttcg ggcgcgccag tagaatcagg
      541 tataaatagg ggatgatgtc tcttgaaaag tccccagggg acagccaaga gacatcgccg
      601 atgattgcat cggtttacgc cgacaattga agttttcgga gtgcaactca gcaagctcgc
      661 gcctggatca atgaaggggg aatgggcacc gttgctcatt tggcgatgct cttgaaaccc
      721 tcaagagact atcaccgtcc gcgagtatag accggatggc gcgggtccag aggcatgcca
      781 tcaaggccaa tatcggggcg gaaaccgtgc gtctcgataa accgcttcgt ggagtcgtgg
      841 cagcgcttgc acaggctctg gaacggaccg agccagaatt tgttcacgtc gccatgatgt
      901 ggctcgacgt gatcgcagat tgtcgctggt gtgacgatgg ctcgctccag gcaatactta
      961 caaagcggat gttcgagcag ctggtgcctc cggatgcgag cccagcaagc cgtggagtac
     1021 cacttatccc acttcgtcgg ttggccgcga acgctcatgg ctcaatctat catccggcgc
     1081 ccgaagcacc aagcaccggt cgccttgcgg tcggggaggt gcaacaacgg aggacggagt
     1141 gcatcccgat acggcgcacc gccttgcgaa ccgtccgagg tcctgcgggg agcaaatgcc
     1201 ggtagagcct cgctcgttgt tcacatcgaa aatcgccgcg gtgcgcaggg cgtcgatact
     1261 cgaacgcaca ccggcgagcg cccacaacct caagaccgcc agaaacgctc ggcctcatca
     1321 tactgctgcc gctgcttggc cgcgccgacg agatgatcct catcacgctg ctcgccggca
     1381 gtctgtcgcc gtcccagcca tgatgcgtct tcagcagaac taccaagccc gctcaagcgg
     1441 gcatacagtg gacatcgaag ctgccatgat gcgtgagccc gcgatatcga acctgggtgc
     1501 ttcatacgcc ctcgcggatg acggcagcgg gaatgtacgg ccaaaccacc atgaagcacg
     1561 ttgccctatc aatagccctt gcatttttcc ctgtgacaac gtcggccgca gcggaagagg
     1621 cgcaaagctt cccgaaggac cagatcgcga ccggcgcgga gctctacgcg gtcaactgct
     1681 cgccgtgtca cggcgcgcgc atgcaggatc ctaacgcggc attcaacctg cggaaatttc
     1741 ctctccacca gcacgagcgc ttcgtcaatg cggtgacgcg cggcaagaac cagatgccac
     1801 catgggggga tttcttcaag cctgatcagc tcgaggcctt gtgggcctat atcatggcgg
     1861 gagagcgatg acccccgact gcacttaacc gtctcgccga cactgctcgg ctgcggcgac
     1921 tcaaatgggg cctcgttgtg ccatcccaac accgtagaag ccgtaaatcg gtccgacaat
     1981 tttgatatat aaagcgccaa cgcggacgat ctcgtcatcg ctggtaaaat gacccatcca
     2041 caaactagga gagaacgcga cgagcaatcc gattgacgcg catgctgctc cgacagttgc
     2101 ccctcccgtc catgcgattt ggtaggcacg atcgtaccgc ttggcgcccc agttggtgcc
     2161 gaccatcgcg acgatcgcag tgccgaaacc gagtgccagc gggataagga tgtattcacg
     2221 gcgcgctccc ctcggcaccg gccggaccaa gggctggcat cggcccccat ccgaaaatca
     2281 gcaagggcga gatgagaata tgcgcgatca cgctgccgac gatgacgccg gcgggcagag
     2341 ccatgttgcc tgtccatcgc acagcgctgc agagtaaatt cagcatacag atggacactg
     2401 cgccgctgaa cgccacgttc gcgtaggcga gcgcaaaaac gattgctccg cgctgatcga
     2461 ggccgacaag atggaaacta ttcttaccga gatcgacgcc gatcgtgcgg atcggcggtg
     2521 aatggtgcgg acctgtgtga ttgccggttg gcattcgaga gtcatgcgcg aagccggtca
     2581 ctggaccgcc accagcctcc ggtgcatcct gcatagtcga tgccatgtgt cagtgccttt
     2641 gcggttttag ccccggcgag cgtcggcagc ggtctcgcgc tcatagcaca tgtcgcgaga
     2701 tggccccgca ggacttcggc gccgtgctgg cgagcagcgg cgccttgttt gaggcgctcg
     2761 ccccagcaga ccggcgtgaa agctcaagtg aagctcgtga aagcagctcc tgactacgga
     2821 gcggacaagc cgcggcctta ctcccccgcc tctccggtga tcacataggc ccatagcgcc
     2881 tcgatctgtt cgggatcgag cacatcatcc cacgcaggca tgttgcgctt gccgttggcc
     2941 accgcatcga cgaaacgcgc gtgtccgtca cgcggaaaag tacgcaggtc gactgcccat
     3001 tgcggattac gcatcctcgg ttgcacaatt gcgcgcaaac agatcggcgc ccttcttgat
     3061 ctgttccgcc gggaattccg gcgcgcgctc ttgagctgct gcgttcacgc cgaggagcgc
     3121 gaaaccgagg ctcgcggtca caaaagcgtt cgcgatcggc tgtgtcattc gggcatcagc
     3181 gcgaacgtcg tcaccgaacc acccaccgga atgaggtcat aatctttcat cctccggttg
     3241 acggagccgc cgacgccgga aagcacggtg acgaattgac gtccctggtg ggtgtat
//
LOCUS       DATLJT010000417         4757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1632089,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000417 DATLJT010000000
VERSION     DATLJT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632089"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..2095)
                     /gene="tkt"
                     /locus_tag="VM910_09610"
     CDS             complement(41..2095)
                     /gene="tkt"
                     /locus_tag="VM910_09610"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVI62823.1"
                     /translation="MDQLCVNTIRTLAMDAVQAANSGHPGTPMALAPVIYCLWQRVLR
                     FDPSHPIWPNRDRFVLSVGHASMLLYAMLHLSGVRAVNPKYETLGRLSVTLDDIKSFR
                     QLDSKCPGHPEYRWTSGVETTTGPLGQGLGTSVGMAIAERWLASYFNRPGFDLFDYDI
                     YALCGDGCMMEGISGEAASLAGHLKLNNLCWIYDNNHITIEGNTALAYSDDVATRFIG
                     LGWNVTRVGDANDLEMLERAFRTFKSTGDRPTLIIVDSHIAYGAPNKQDTSAAHGEPL
                     GEEEIRLAKRHYGWPPDAKFLVSDEVRKHFEEGIAARGLALHRAWWAQFEEYRRQYPE
                     LADQGYRMLRRELPDGWDGGWPTFSTDSKGVATRDASGQTLNTFARQVPWLLGGSADL
                     GPSCKTRLAFEGAGDFSAENPAGRNLHFGIREHAMGAILNGLSLSKIRPFGSGFLIFS
                     DYGRAAIRLSALMEIPVIYIFTHDSIGVGEDGPTHQPVEHLASLRAIPGLITLRPADA
                     NETVEAWRVIAEFRHEPVALILTRQTVPTLDQTKYAAAEGVRRGAYILADADDSRPQV
                     LLLATGSEVALCVDAYETLKAEGIKARVVSMPSWEMFEHYCRDHPEYRERVLPKAVTA
                     RVSVEQGSTLGWSRYVGDSGHSIGMETFGASAPLKELQKRFGFTPESIIATAKHQIAG
                     AR"
     gene            complement(2269..3009)
                     /locus_tag="VM910_09615"
     CDS             complement(2269..3009)
                     /locus_tag="VM910_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVI62824.1"
                     /translation="MIKDVLASGPSGKKVLVIDVGGTSVKILASGQTEIRSFRSGPTL
                     TPVRMVSGVKKLAADWTYEVVSIGYPGLVLGGRPTADPSNLGRGWVGFDFAAAFGRPV
                     KVVNDAAMQAMGSYKGGKMLFLGLGTGLGTSLIVDGKVKPMELGHLPYKKGTYESYVG
                     RTGLERDGKKKWRRRVADVVERLIAALQPNETVIGGGNVRKLDALPPRCRGGHNANAF
                     RGGFRLWTKDNSIPPDLPPARYRSNRKA"
     gene            complement(3222..>4757)
                     /locus_tag="VM910_09620"
     CDS             complement(3222..>4757)
                     /locus_tag="VM910_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HVI62825.1"
                     /translation="LCGRAEWTLDRVVTQGFQRLLASQEQYMDDFWRRSDVRIKDIRE
                     DRIKRSTVEIQQAIRFNLFHILQASARAEETSVPAKGLTGQAYEGHYFWDTEIYLLPF
                     LIYTSPRIARNLLAFRYKLLPQARARAKELGHRGAMFPWRTISGEEASAYYAAGTAQY
                     HINADIMYALRKYVQATGDEAFLRNFGAEMLVETARLWVDLGFYSDAKGEKFCINGVT
                     GPDEYNAVVNNNAYTNLMARENLRYATGVIESLRATEPDAYNALVHKTALESSEVKAW
                     IRAAENMYVPYDEKLKIIPQDDEFLDKERWDFRNTPADHYPLLLFYHPLNIYRKQVIK
                     QADVILAMFLLGDVFPPETKKRNFDFYDPLTTGDSSLSSCVEAIIAAQIGDMEKAFRY
                     GMAALLMDLADVGGNAKDGCHIASMGGTWMMLTYGFGGMRDNDGTLSFWPRRAPEENA
                     ILRFPVTYRGQRLEVEIGLDKVEYALREGECLVIRHETEEVQLSRENPVAVRPVSRRI
                     AVS"
BASE COUNT          927 a         1492 c         1324 g         1014 t
ORIGIN      
        1 aaatccggca tgatctgaac cgatcgcgat gcgcatcgtt tcacctcgct cccgcgatct
       61 ggtgcttcgc cgtcgcgatg atgctctcgg gggtgaagcc gaacctcttc tgcaattcct
      121 tcagcggcgc cgaggcacca aacgtctcca tgccgatgct atgaccgctg tcgccgacat
      181 accgcgacca gccgagggtt gatccttgct cgacggagac gcgcgcggtg accgccttcg
      241 gcagcacccg ctcgcgatac tcggggtgat cgcgacagta gtgctcgaac atctcccatg
      301 acggcatact caccacgcgg gctttgatcc cttcggcctt cagcgtctcg tacgcatcta
      361 cgcagagcgc gacttcactg ccagtcgcca ggagcaacac ctgtggccgg ctgtcgtcgg
      421 catccgcgag aatataggcg ccgcgccgca cgccttcggc cgccgcatat ttcgtttggt
      481 ccagcgtcgg caccgtctgc cgggtcagaa ttaaggcgac cggttcgtgg cggaactccg
      541 caatgacgcg ccaagcctcg accgtttcgt tggcatcggc cggtcgcagg gtgatgagtc
      601 cagggatggc gcgaagcgac gccaagtgct ctaccggttg gtgtgttggc ccatcttctc
      661 cgactccgat cgaatcgtgg gtaaagatgt agatcaccgg gatttccatc aacgcgctca
      721 aacggattgc cgcccggccg tagtcgctaa agatcaggaa ccccgatcca aacggtcgaa
      781 tcttcgacag tgacagacca ttgaggatcg cgcccatggc gtgctcgcga atgccgaagt
      841 ggagattccg accagccggg ttctccgcgc tgaaatctcc ggcgccttca aatgccagcc
      901 gtgtcttgca tgagggcccg agatcggccg aaccgccgag cagccacggc acttgcctgg
      961 cgaacgtgtt cagtgtctgg cccgaggcgt ctcgagtggc cacgcctttg ctgtcggtag
     1021 aaaaggtcgg ccatcccccg tcccagccat ccggcagctc acgacgcagc atgcgatatc
     1081 cctggtccgc aagctccgga tactggcgtc gatactcctc gaactgagcc caccaggctc
     1141 ggtgcagcgc caagccccgc gccgcgatgc cctcctcgaa atgcttacgc acttcatccg
     1201 aaacgaggaa cttcgcgtct ggcggccagc cataatgacg cttcgccagc ctgatctctt
     1261 cctcgcccag cggttcgccg tgcgccgcgc tcgtgtcctg cttgttcggc gcaccgtagg
     1321 cgatgtggct gtcgacaatg atcagagtgg gtcgatctcc cgtgctcttg aacgtcctga
     1381 acgcgcgctc gagcatctcg agatcgttgg cgtcgcctac gcgcgtgacg ttccagccga
     1441 gtccgatgaa gcgcgtggcc acatcatcgc tgtacgccag cgccgtgttg ccctcgatgg
     1501 ttatgtggtt gttgtcgtag atccagcaaa gattattgag cttgaggtgt cccgcgagcg
     1561 aggcggcctc gccggaaatc ccttccatca tgcacccgtc gccgcacaga gcgtatatgt
     1621 catagtcgaa aagatcgaag ccgggacggt tgaagtagct ggccagccag cgctccgcga
     1681 tggccatgcc gacactcgtc cccagcccct gtcccagcgg gcccgtcgtc gtttcgacgc
     1741 ccgatgtcca acggtattcc ggatggccgg gacatttgct gtcaagctgt cggaagctct
     1801 tgatatcgtc cagcgtcacc gacaatcggc caagcgtttc gtacttggga ttaaccgccc
     1861 tgaccccgct caggtgcagc atcgcgtaaa gcaacatcga tgcgtggccg actgacagca
     1921 cgaaacggtc acggttaggc cagattggat ggctggggtc gaagcgtagg acgcgttgcc
     1981 atagacaata gatcaccggc gccagcgcca tcggtgttcc cgggtgacca gaattagctg
     2041 cttgtacggc atccattgcc agcgtgcgga tggtgttgac acacaactgg tccatcgtat
     2101 cgttcgtcga tttggtaaca ctcggtaccg agtgtgcagc gaggtcgatt gtgctcatcg
     2161 atcttgctcc caaaagtgtg gagcgcgggc gtgcagtgag tttgccagtc aatcgcgtgt
     2221 cggcaagctc tccgatcgtg cagcaatctc cagcacccgt tccgatcctc aggcctttcg
     2281 gttggaccga tagcgggcgg ggggcagatc cggcgggata gaattgtctt ttgtccacag
     2341 ccgaaaacca ccgcggaagg cattggcatt gtgccccccg cgacaacgcg gaggtagtgc
     2401 gtcgagcttt cttacgttgc ctcccccgat caccgtctca ttgggctgca gcgccgcgat
     2461 gagacgctcc acaacatcgg ccacgcgccg ccgccacttc ttcttaccgt cacgttccag
     2521 gccggtgcgt ccaacgtagc tctcataagt gcctttcttg tatggcagat ggccgagctc
     2581 cataggcttc accttgccat cgacgatgag ggaggtaccg agtcccgtcc caaggcccag
     2641 aaacagcatt ttgccgccct tgtagctgcc catagcctgc atggcggcgt cgttgacaac
     2701 cttcacggga cgaccgaagg ctgcggcgaa atcgaatccg acccagccgc gtcctagatt
     2761 cgaggggtcc gcagtcggcc gcccgcccaa aaccaggccg gggtagccga tggacaccac
     2821 ttcatacgtc cagtccgcgg cgagcttttt cactcccgaa accatccgca caggagtgag
     2881 cgttggaccg gaccgaaaag atcgaatttc tgtttgcccg ctggcgagga tcttcactga
     2941 agtgccgccc acgtcgatga ccagcacctt cttgcctgag ggaccgcttg caagcacgtc
     3001 cttgatcaag aatcccagtg cggcatgccc tcgagcttcc ccctttgttg cgcacccgcg
     3061 cttggcagca ggcgatgact taacgctcat aacactagct catccgaaga ggatggcggg
     3121 cttggatttt gatcatgttc atcagttcca cgaactaacg atgaggtaac gccctcctct
     3181 agattcacgt cgtcgcaagc gactgcgcaa ccactcaacc actaactgac agcaatcctc
     3241 ctgctaaccg gtcggaccgc gactggattt tcccgactca actgaacttc ttctgtttca
     3301 tggcgtatca caaggcattc gccctcacgc aacgcgtatt ccaccttgtc cagaccgatt
     3361 tcgacttcca gcctctggcc gcggtacgtc acaggaaacc gaagtatcgc gttttcctca
     3421 ggcgcacgac gtggccagaa tgacaaggtc ccatcattgt cccgcatgcc gccgaaccca
     3481 taggtgagca tcatccaggt gccgcccatg gacgcgatat gacaaccgtc cttcgcgttg
     3541 ccgccgacgt cggccaaatc cattagcagt gccgccatgc cgtaacgaaa cgctttctcc
     3601 atgtcgccga tctgtgcggc gatgatcgcc tcgacgcatg acgacagcga tgagtcgccc
     3661 gtcgtcaatg ggtcgtagaa atcaaagtta cgcttcttcg tctccggggg gaaaacatct
     3721 cccaacaaga acatggccaa aatcacgtcc gcctgtttga tcacttgctt tcgataaata
     3781 ttcagcggat ggtaaaacag cagcagggga tagtggtcgg ccggcgtgtt ccggaaatcc
     3841 cagcgctctt tgtccagaaa ctcgtcatcc tggggaataa tcttcagttt ctcgtcatac
     3901 ggcacataca tgttctctgc ggcgcggatc catgctttca cctcagagga ctcgagcgcc
     3961 gttttgtgca caagcgcgtt gtacgcgtcc ggttctgttg cccgcaagga ttcgataacc
     4021 ccagttgcgt agcggaggtt ttcacgggcc atcaagttcg tatacgcgtt gttgttcacc
     4081 actgcgttgt attcatcggg cccagtcaca ccgttaatgc agaacttttc ccctttggcg
     4141 tcggaataaa agcccaggtc cacccacagg cgcgccgttt ccacgagcat ctcggcgccg
     4201 aagtttcgca gaaacgcttc gtcgcccgtt gcctgcacat acttgcgcag cgcgtacatg
     4261 atgtccgcat tgatgtggta ttgtgcggtt ccggcggcgt agtatgccga tgcttcttcc
     4321 ccgctgatgg tgcgccacgg gaacatggct cctcgatggc ctaattcctt cgcgcgtgct
     4381 cgtgcctgag gcagcagctt gtagcggaag gccaggaggt ttctggcgat ccggggggac
     4441 gtatagatga gaaatggcaa cagatagatc tcggtgtccc aaaagtaatg cccttcatag
     4501 gcctgtccgg taaggccttt cgccggcaca ctcgtctctt ccgcgcgtgc cgaagcctgc
     4561 agaatgtgaa aaaggttgaa gcggatcgct tgttggatct ccacggtgct gcgctttatt
     4621 cggtcttccc tgatatcctt gatccggaca tcgctccgac gccaaaaatc gtccatgtac
     4681 tgctcttgag aggcgagcag ccgctggaat ccctgggtca ctacgcgatc caatgtccat
     4741 tcagcacggc cgcagag
//
LOCUS       DATLJT010000418        13252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3699942,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000418 DATLJT010000000
VERSION     DATLJT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13252
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3699942"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VM910_09625"
     CDS             complement(<1..257)
                     /locus_tag="VM910_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62826.1"
                     /translation="MRDRVRLMLGVAVAVAMAISAGRTHAEDPNAAPNPYGAVENWAK
                     LPEGRAWGQAIGVDIDRDGTSVWVYDRCGAKTCEGSNIPPI"
     gene            726..884
                     /locus_tag="VM910_09630"
     CDS             726..884
                     /locus_tag="VM910_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI62827.1"
                     /translation="MVYDDQMTGRRPAVLMIHAREGMTPKTLGLAETWAKLGYVSFAA
                     DIFGYRQL"
     gene            complement(1031..1558)
                     /locus_tag="VM910_09635"
     CDS             complement(1031..1558)
                     /locus_tag="VM910_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62828.1"
                     /translation="MALSLRNVRCDPIANRALNELMHHYTVAQEKSRLVLTKKTGGMK
                     LFLNDLDDLYQWDFIRNQQMVKQIERLRVLLTTTNQHRESWKVRALMAEAQLLEATVK
                     TGNKGGGENVSDVRYASLKRYLAKRFHPDHAPGQGIEKLVRNEIFKEIWNEIDRLDQG
                     VSATRGATARSAAAA"
     gene            complement(2057..2302)
                     /locus_tag="VM910_09640"
     CDS             complement(2057..2302)
                     /locus_tag="VM910_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62829.1"
                     /translation="MTIDEKFMAHARECVRLAGLTDDQEVRHQLIALARDWIVIARKQ
                     EEEDDDSKVLVFPSAVERNRRHGLRISLRHAGPRRLS"
     gene            2372..2542
                     /locus_tag="VM910_09645"
     CDS             2372..2542
                     /locus_tag="VM910_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62830.1"
                     /translation="MSASQLLISGIAERGHHRRALSSILLKSGKVKGVLYLGRRENAT
                     SDERSRAVSRGD"
     gene            complement(2539..2736)
                     /locus_tag="VM910_09650"
     CDS             complement(2539..2736)
                     /locus_tag="VM910_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62831.1"
                     /translation="MTIYPVEFQRRLEQKWASRIERILPDIGTTTPPRDPNDDDDNED
                     EDEEDEDQPDQPAVVREPDED"
     gene            complement(3113..3340)
                     /locus_tag="VM910_09655"
     CDS             complement(3113..3340)
                     /locus_tag="VM910_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62832.1"
                     /translation="MTKRRRSATTRSVPAQTPEQLLELAQEHIRPLIGVIDRLNEADA
                     EDELRVISKQLARLSFRVQPHFRVQPFPSDT"
     gene            4229..4573
                     /locus_tag="VM910_09660"
     CDS             4229..4573
                     /locus_tag="VM910_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62833.1"
                     /translation="MQAAMPKRKRHSRVEIATKLAQADELATQGKLQREIARTLGVSV
                     MTLHRWHRAPPGPQCALVVTPKAREPDRTRADDDRIAELQLENARLRQLVTDLLLEKI
                     KVIASASRNWRT"
     gene            4776..5393
                     /locus_tag="VM910_09665"
     CDS             4776..5393
                     /locus_tag="VM910_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62834.1"
                     /translation="MPTEQLTYAQIAERLSVSPEAARAIVKRHRLPRSRRNDGKTLAA
                     IDLKEIRHTPMPVRSPRGRRSVTDVVATLKARVAGLETELAQAEKRSAGHRVDFERER
                     ERADQLVTTQDRMVAELEALHSLLRSLLEAAQPAASPVTSRTPDMARRDMARDDSLAQ
                     SMRSLLEAAHAARLEAARQPARPVTSRTWREMTWSERWRRLRRTG"
     gene            complement(5627..6511)
                     /locus_tag="VM910_09670"
     CDS             complement(5627..6511)
                     /locus_tag="VM910_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI62835.1"
                     /translation="MMPCLLDRGATRSCRRGDRITRLMCYSFVGSTSAGRPGANHETF
                     LIRHLATLLCPMAASAQLNVLISGGFSGAYEQLLPEFERTSGIKVTTGSGASQGTGPQ
                     TIAAQLARGVPADVVILSREGLSELIAAKRIAAGTDVDLARVPLGVAVRAGTPKPDVS
                     TVEAFRQVLLKAKTVAVPGSTSGIWLTNDLFPRLGIAEKINVKAAPRGKDATGMVAAG
                     GADLAVMPISEILNAPGVDFAGSIASEIQFVQTFSAAVVASSGDIERSKRLIEFLASA
                     RASEAIKKSGMEPLAAAR"
     gene            6890..7726
                     /locus_tag="VM910_09675"
     CDS             6890..7726
                     /locus_tag="VM910_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62836.1"
                     /translation="MSYRGSICALPLAAALIATPSAAQVVDFGKYPDLKGQWVRPPGS
                     PNNWLRLAGPPPLTPEYRQIWEDIQADLKAGGPGNWPSTFCIPAGMPAMMSFYDPAEI
                     IITPETTYILISHNDDSYRRIYTDGRAWPAEAAPTFAGYSIGKWIDEDGDGKFDVLEV
                     ETRFLKLPRSYDISGVPFHNDGQAIIKERIYLDKADRNTLYDEITVIDHALTRPYSKT
                     QKATRNPDPRPVWYSDVCSENNPHIRIGNEPYYLSADGKLMPSKKDQAPPDLSYFKRT
                     GK"
     gene            7978..8361
                     /locus_tag="VM910_09680"
     CDS             7978..8361
                     /locus_tag="VM910_09680"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HVI62837.1"
                     /translation="MRQRLSVILISGTLIAMGGAAMAHHSFAMFDNENQIELEGVVRE
                     FKYTSPHTFILLEAKSQDGSITVWNLEGVTPSALAREGWSSQSLKAGDKLKMTIAPLR
                     SGAPGGAWTPQGTRFRDGKEVVISP"
     gene            complement(8667..8888)
                     /locus_tag="VM910_09685"
     CDS             complement(8667..8888)
                     /locus_tag="VM910_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62838.1"
                     /translation="MASNVRFWLKADTFVEAGGLISYASRNTERERQVGIYVGRILKG
                     EKPADLPVMQPTKSGTGYREIPLAWNPTI"
     gene            9183..9707
                     /locus_tag="VM910_09690"
     CDS             9183..9707
                     /locus_tag="VM910_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62839.1"
                     /translation="MALSLRDVRCDPIANRALNELMHHYTVAQEKSRLVLTKKTGDMK
                     LFLNDLDDLHQWDFIRNQQMVKQIERLRVLLTSTNQHRESWKVRALIAEAQLLEATVK
                     TGNKGGGEKLSDVRYASLKRYLAKRFHPDYAPGQGIEKIVRNEIFKEIWNEIDRLDQG
                     VSARGATARSAAAT"
     gene            complement(9920..12430)
                     /locus_tag="VM910_09695"
     CDS             complement(9920..12430)
                     /locus_tag="VM910_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI62840.1"
                     /translation="MGRRGTISRKPAKTRHGNKAKRARSNARTAAPPASSTVADLQER
                     VSALTRELVEAREQQTAASEVLSVISRSPGELKPAFEAMLANAMRICEAKFGHLLLYD
                     GEHVHAAYLHDVSPAYRELWERGPLCPPPGSGIRRLVDTKQVVHITDLAAEPAYAERE
                     PLRVATVELEGARTFLGVPMLKENRLVGAIIIYRQEVRPFTDKQIELVQNFAAQAVIA
                     IENTRLLNELRQRTDDLTQSLEQQTATAEVLMAINSSAGELAPVFDTMLEKAMSLCEA
                     AFGGFWTYDGEHIYATAMRGVPALLTMFYNKPLVPAPGNASYELVRGENFVHIPDITE
                     DEGYRSGNPAKRALHEMGGARTALFLPLRKDRSLLGTFMIYRQEVRPFTDKQIALLQN
                     FAAQAAIAMENARLLNELRQRTDDLSESLEQQTAASEVLRVISSSPGELEPVFQTMLE
                     NATRICDAKFGTIFRFDGDAFHPMAMVNTPPALTNFLRRRGRFVPEAGNGLYRVWKSK
                     KPLHTLDDAASPVPTAPTKLAGARTYLGVPMLKDDELIGVIVIYRQEVRPFTDKQIEL
                     VKNFAAQAVIAIENTRLLTELRARTAELEDKSQQLEVASENKSQFLSSMSHELRTPLN
                     AIIGLTEMMVTNAARFGTDKALEPLRRVNAAGTHLLSLINEVLDLSKIEAGKLDLNPE
                     SINLSHLIDEVVGTAGQLTEKNKNRLVVEAPENVGTLTADSMRLKQILLNLLSNACKF
                     TKDGEVALRVRKVVDRRDWIELAVADTGIGLTAEQQAKLFQDFTQADSLTARRYGGTG
                     LGLALSRKLARMMGGDVTVTSEPGKGSVFTVRLPGADS"
     gene            complement(12656..>13252)
                     /locus_tag="VM910_09700"
     CDS             complement(12656..>13252)
                     /locus_tag="VM910_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI62841.1"
                     /translation="PGLYVYHCATPMVAEHIANGMYGLILVEPEGGLPPVDREFYVMQ
                     GEIYTDIPHGQHGSAEFSVEKLLNERPEYFVLNGSAGALSKLHPLHAKVGETVRIFFG
                     VGGPNYTSSFHVIGEIFDKVYMLGGAQTAPLEGIQTVTVPAGGAVIVDFKLEVPGNYT
                     LVDHALSRMERGLVGILSVEGQPNPEIFNGQVMPGMGH"
BASE COUNT         2672 a         3976 c         3859 g         2745 t
ORIGIN      
        1 tggatcggcg ggatattcga gccctcgcag gtctttgcgc cacagcgatc gtaaacccag
       61 acgctggtgc catctcgatc gatgtcgact ccgatcgcct gtccccacgc acggccttcg
      121 ggaagctttg cccaattctc cactgcgcca taggggttgg gcgcggcatt gggatcctcg
      181 gcatgggtcc gccccgcgga aatggccatc gccacggcga cagcgacgcc aagcatcaat
      241 ctcacacgat cacgcatgtc acttcctccc ttttgcaaat tgcacatgcg ctgttctcga
      301 ccagcttgaa ctgatacttc cctcacgttt acctcagccg ctgtactatt gccgcgccaa
      361 ggccgtcgcg atacttggca gcatcatcgc gagcccgcca agagcgacgc tcacaagcgc
      421 tgcgatcgtc attttagctt tcacaacttc ctctggtggg agacgttagc accgctcgag
      481 gcgttcgtat tcctatttca gcgcatgaca gcaccacttt cattcatcct ggcgtctgga
      541 cttgagctct cactcgcgca aaactttgcg aaaccgctta tgccagggag gcttcaatga
      601 agatgcgccg gacgcttcca gccttcttaa tcgccgcctt tgcgctcgct acgagcccag
      661 cacaggccga gatgaagagc tagtgggtcg aatacactca cggcgacacc aagctcaaag
      721 cctatatggt ctacgatgac caaatgactg gccggcgtcc tgccgtgctg atgatccatg
      781 cgcgcgaggg catgactccg aagacgctgg gactcgcgga gacctgggcc aaactcggct
      841 atgtctcgtt cgcggccgat atattcggtt atcggcagtt atgaagcgcg tcagcacaag
      901 ctcgcttcat caaccgtgag tcaggcggaa gggaaccact tgcctcgaga acgcgcggtg
      961 ttcgagaagg atctcagccc acgcggcgcg cgcgtcgttg caaggccgcg cggagcggca
     1021 aattgcccga tcaggccgcc gcagctgatc gcgccgtcgc gccgcgggtg gccgagactc
     1081 cttgatcgag gcgatcaatc tcgttccaga tttctttgaa gatctcgttt cgaacaagct
     1141 tctcgatgcc ctgtccgggg gcgtgatcgg ggtggaaccg ctttgccaga tagcgcttga
     1201 gcgacgcata cctgacgtca ctgacattct caccaccccc cttgttgccg gttttgacag
     1261 tcgcttcgag taactgcgcc tctgccatca aggcacggac cttccaactc tcccgatgct
     1321 gatttgtcgt ggtcaatagc acacgcagtc gttcgatctg cttcaccatc tgctgattac
     1381 ggatgaaatc ccattgataa aggtcatcga ggtcattcaa gaagagcttc atgccccctg
     1441 tctttttggt cagaaccaaa cggcttttct cttgtgctac ggtatagtgg tgcatgagct
     1501 cgttgagagc gcggttcgca atcggatcgc accgaacatt gcgtaagctg agtgccatgc
     1561 gtgcccccca tttacgagca aattccccgc ataatttact gatgaatgtg accgcttggc
     1621 ggccagatgg aactcttgca gcgccaacac aaatcgattg gcgcaagtgg cgcccctgga
     1681 gctagggagc taaattggat gacaactgcg cccgggccgg ggctggataa aattcgggat
     1741 cgtctgaaat aggcgattca gaacgcgtct cggggcttta gctcggcgcc gccgcggccg
     1801 cgagcttcat ctcggccagg agccaaaacc aagcgcattg aacgatataa tcctcgacgt
     1861 gatggaagcg gacgacaaga acgactacga tgtggcagac atcgtgtgcg aggagtgcaa
     1921 gtccatcgtg acgctgcagg ctcagcgggc caattgaagc ccgcgtggtg cgccgatgaa
     1981 cgatgacgag cgaacccgtg cctgtaaggc gcagtccgaa agcggtcgtt acgttgcggc
     2041 aaggcgcgag ggttattcag gaaagccgtc gcggacctgc gtgacgcagg gagatccgta
     2101 gcccgtggcg gcggttacgc tcgactgctg acgggaagac cagcactttg ctgtcgtcgt
     2161 cctcctcctc ctgcttccta gcaatgacaa tccagtcgcg ggccagggcg ataagctgat
     2221 gacgaacttc ctggtcgtcg gttagtccag ctagccgcac gcattcgcgc gcgtgagcca
     2281 taaacttctc gtcgatggtc atacgtgaca gccgcagtga gggggtttgc ccccggcaga
     2341 cggttacgtt tattcgcttg ggcgggccag aatgagcgcc agtcaactgc tgatcagcgg
     2401 cattgcggaa cgcggccatc atcgccgcgc gctctcctcg attctgttga agtcgggcaa
     2461 ggtgaagggc gtgctgtacc tagggcgccg cgaaaacgct acatcagacg aaagaagtcg
     2521 ggccgtgtcc cgaggcgact agtcttcgtc gggttcgcgg acgaccgccg gttggtcggg
     2581 ctggtcttcg tcctcttcgt cttcgtcctc attatcatcg tcgtcgttgg gatctcgcgg
     2641 cggtgttgtc gtgcctatgt cggggagaat ccgctcgata cgacttgccc acttctgttc
     2701 caggcgtctc tgaaactcca ccggatagat cgtcatgtcg tcctctcgac gtgaattttt
     2761 gaaggctcgg cgcgtttagg ttcctcgccg agcgcgaggg cctaatacat ctcggcaagt
     2821 ttcccgtatt ggcggctatg ccggagcgat tttgcaatca ccgagagacg agcgaactct
     2881 ttcgcttggc tcgataattc tctatttgcg actgcacggc agaccactcc gactgcacct
     2941 ttgcgcagat taacgtgcag gtgccagcct ctgttccgcg ttctgcggac agataccagg
     3001 aggatgtacg gatgttatcc gatttgtccg ctgtaattag cggccccacg aggtatccag
     3061 agcgggtagt cgaatagccg ctgcccggtg gcgtgccgag cggctcttgg ggctacgtat
     3121 cgctggggaa cggttgcacc ctgaagtgcg gctgcaccct gaaggacagc cgcgcgagtt
     3181 gtttgctgat aactctcagc tcgtcttccg cgtcggcttc gttgagccgg tcgatcaccc
     3241 cgatcagcgg gcggatatgc tcctgggcca gctcaaggag ttgctcgggg gtctgggcag
     3301 gcaccgaacg agtagtggca gaacggcgac gcttggtcat gattacccct cccttcgtac
     3361 cggtaggaga taacgttacg tcagccgcaa ggttcctttt cttctggagc gcattttccg
     3421 cagcagagcg cgcgacatta gggtccgcgc ggttttggtt taccgttcca aatttctcga
     3481 ttgcctacgc ggtgcggtaa cgaatagcta caccacccgc aggcgccggc tgtgctgtcg
     3541 cctgtgtcgt cccaaatgtc cacgcggaag gcaaaacttt cgcccaacac gcgcaattaa
     3601 aatctattgc ggcttgtcga accatcgcgc gaggcccgaa tctggaggaa gagatccacc
     3661 acactgcctc gaagctgtgc attctccgct tcgagccgcg caagtaggct ctgattcaga
     3721 tgcactacag ccccagaggt atgaagatat gtaacattgg aaccgtctct tgattgccaa
     3781 ggacgctcaa gtgcaagctg caggctctgc acatcctctt ttctgtttga tcgagatttt
     3841 ttgttcattg gtaaccctcg gttggatggt acccgttgcg cgatgcccct cagagccgta
     3901 cgcgaagatc gcatactttg acaaaccatt tgcgattggc cactcacatt tcgtgatgtg
     3961 cagtaagatt tgcggcgcag atggggtgta aaaatgggag gtggaatttg cagcacccag
     4021 cagtgtggcc cgcttgctac acaccgccgg atttgcaatc tcgccaacac aggtctcgca
     4081 tttcattacc tgcgcaccac acagcggccg cacccctcgt gttaaatctg caacgcgata
     4141 actggttgcc attgttgggc ggggaatgcg ttgtgcagca cctaacgcgc aaaaagagat
     4201 ttgcggcaca acgtagggag ggggtcgaat gcaagctgct atgcctaaaa ggaaaaggca
     4261 ctcgagggta gaaatcgcga cgaagttggc gcaagcagat gaattggcaa cacaagggaa
     4321 actgcaacgc gaaattgcgc gcacacttgg cgttagtgtc atgactttgc atcggtggca
     4381 cagggcgccg cctgggccgc agtgcgcatt ggtggtgacc ccaaaggcga gagagcctga
     4441 tcgcacgcgc gccgacgacg accgaattgc cgaactccag ctcgagaatg cacggttgcg
     4501 gcaactggtg accgatctcc ttcttgagaa aatcaaggtc atcgcttcag ccagccgcaa
     4561 ctggcgcaca tagcacggca ggcatgggcg tggattaaat aaagttgggg attgcaactt
     4621 actatcaaaa gcgcgctttt tttttgctta gggtcgtctc ggtcatcatg cccttgggga
     4681 gaggggtgcg ttgtgacaag tcccggcccg tcgccgccct ttgacgggcc agggcgtcac
     4741 cgcgcgggca ccgggccgtc acctccgggt caatcatgcc taccgaacaa ctcacttacg
     4801 ctcagatcgc tgaacgcctc agcgtctccc ccgaagctgc tcgcgctatc gtcaagcgcc
     4861 atcgcctgcc gcgctcgcga cgcaacgatg gcaagacctt ggccgcgatc gacctcaaag
     4921 agatcaggca cacgcccatg cctgtgcggt caccgcgagg tcgccgatcg gtcaccgacg
     4981 tggtcgccac cctcaaggcg agggtcgcgg ggcttgaaac tgagcttgcc caggccgaaa
     5041 agcgctcggc tggtcaccgg gtggacttcg agcgcgagcg cgagcgcgcc gaccaactgg
     5101 tcaccacgca agaccgcatg gtcgccgagt tggaagccct ccactcactc ctacgctcac
     5161 tcctagaagc cgcacagccg gctgcctcgc cggtcacctc ccggaccccg gacatggcgc
     5221 gacgtgacat ggcgcgagat gattcgctgg ctcaatcgat gcgctctctt ctggaagccg
     5281 cgcacgctgc tcggctggaa gccgcgcggc agcctgctcg gcccgtcacc tcccgaacat
     5341 ggcgcgagat gacgtggtcc gagcggtggc gccggttgcg caggaccgga tgacgcgggc
     5401 ttttgtcaac attccccggt cctcccccga aaggcggcgg cgcggggcgc ttccgccatc
     5461 cgcgccttgc ctgatttgtg cctcattcca tttcgcggca gaacgcatga cacgtcgcgc
     5521 ggttactcct tgccgacatc atccgcttaa ttagcgatgt ccgcttcccc cctcaaagcg
     5581 gacacagcgc agcatgttgg catgtccgct ttgtgccaat cgcgtcctaa cgcgccgccg
     5641 cgagcggctc cattccgctc ttcttaattg cttcggaagc gcgcgcggag gcaagaaatt
     5701 cgatcagtcg cttagaacgt tcaatgtctc ctgagctcgc tacgacggcg gctgaaaacg
     5761 tctgcacgaa ctggatctcc gacgcgatgc tgcccgcgaa gtccacgcct ggggcgttca
     5821 aaatttcact gattggcatg accgcaagat ctgcgcctcc tgcggcgacc atgccggttg
     5881 cgtccttgcc gcgtggcgcg gccttgacgt tgatcttttc cgcgatgccg agccgcggaa
     5941 aaagatcgtt cgtaagccag ataccgctcg tgctcccggg gaccgcgacg gttttggcct
     6001 tgagtaggac ttgtctgaag gcctcgacgg tgctcacgtc gggctttgga gttcccgccc
     6061 gcaccgccac gccgaggggt actcgcgcaa gatcgacatc cgttccggcg gcgatcctct
     6121 tcgcggcgat cagctcactg aggccctccc gagagagaat caccacgtcg gcaggcacgc
     6181 cgcgggcgag ttgcgcggca atcgtttgcg gccctgttcc ctgcgacgca cccgatcctg
     6241 tggtgacctt gataccgctc gtccgctcga attccggcaa cagctgctcg tacgcgccac
     6301 tgaatccgcc ggatatcaaa acgttcaact gtgccgatgc cgccattggg caaagcagcg
     6361 tggcaagatg acgaatgaga aaagtttcat gattggctcc tggccggccc gcactcgtgc
     6421 ttccgacgaa gctatagcac attaatctcg ttattcggtc acctcgtcgg cacgagcgag
     6481 tagcgccacg gtcaagcaga catggcatca tcacgaagcc atgtccgctt tacccccgaa
     6541 agcggacaaa gagcagacat gtcggcatgt ccgctttgtg ccaaaagcgg acattcgcat
     6601 tgcagcaaat agctttgcta ttcgatcacc tcgtcggcgc gggcgaaccg cgccgcttga
     6661 atttcgaggc cgagtgcctt cgccacgaat tgacgggctg ctcgaccgga atatcggctg
     6721 gcttggtgcc gcgcgcttca tggctagcgc ctccgatggc tcttgccggt caatatgttc
     6781 gaatctaagt tttcggtaca cacagttact cgatcgcgac attgacgacg ataatcgctg
     6841 atggcgcggc ggccgcgcgt gccccttggc gaatccaagg aggatgccca tgagttaccg
     6901 aggctcgatc tgtgcactcc cactggcggc cgcgctgatt gcgacgccat ccgctgccca
     6961 ggtggtcgat ttcgggaaat atcccgacct taagggtcaa tgggttcgcc cccccggcag
     7021 cccgaataat tggcttcggc ttgccggccc tccgccgctc acacctgagt atcgccagat
     7081 ctgggaagac attcaggccg acctgaaagc cggcggtccc ggcaattggc cgtcgacttt
     7141 ctgcatcccg gccggcatgc cggcgatgat gagcttctat gatccggcgg agatcatcat
     7201 cacgccggag accacctaca ttctgatcag ccataacgac gattcgtatc gacgaatcta
     7261 tactgacggc cgcgcttggc cagcggaggc cgcgcctact ttcgcgggct actcgatcgg
     7321 caagtggatt gacgaagacg gcgacggcaa attcgacgtg ctcgaggtcg agacgcgttt
     7381 cctcaaactt ccgcgcagtt acgacatctc gggcgtgccg tttcacaacg acggccaggc
     7441 catcatcaag gagcggatct atctcgacaa ggccgaccgc aacacgctct atgacgagat
     7501 caccgtgatc gatcatgcct tgacgaggcc gtactccaag acccagaagg ccacgcgcaa
     7561 tcccgatcct cgtccggtct ggtactccga cgtttgctcg gagaacaatc cgcatatccg
     7621 aatcggcaat gaaccctact atctcagcgc cgacggcaaa ctgatgccca gcaagaaaga
     7681 tcaagcgccg cctgatctgt cgtatttcaa acgaactggg aagtgaacgc acgttcgcag
     7741 gcccgggtta ggaatggcgg tcgcacgcgc cgagaagtcc gaccgcattg cctgcgcggg
     7801 accttggcag caaggtagaa cggagtatcc tacccgcaag aagggccttc cacggacaac
     7861 cgagcgacga ttgcagaatg tcggccgcca caagcggaga tgcgctgcag aacccagggt
     7921 tcgcagtggc gttgctgtgt gctcgaagca cttgatccca gcatatgagg aagcgccatg
     7981 agacagcgtc tctctgtcat attgatcagc ggtactttga tcgcgatggg tggcgcggcg
     8041 atggcgcacc actcctttgc gatgttcgat aacgagaatc agatcgaact cgaaggcgtc
     8101 gtgcgggagt tcaagtacac gagcccccac acgtttatcc ttttggaggc caagtcgcag
     8161 gatggcagca tcaccgtctg gaatctcgaa ggcgtgacgc caagcgccct ggcgcgcgag
     8221 ggatggtcca gccagagcct caaagctggc gacaaactca aaatgacgat tgcgccattg
     8281 cgcagcggcg caccgggtgg cgcgtggacc ccgcagggca ccaggtttcg cgatggcaaa
     8341 gaagtcgtga tcagcccctg atccggatcg catcggccgg gtcactggct gcgctgctgg
     8401 atggcccctg acgcatcacc ccagcctagc tcgcgaagtc gctacacgaa cgccgccgac
     8461 cagctgcccc aggccgaagc ggtcgcaccg cccaagaagc cgatcctccc gtgcagcggc
     8521 gtgttgttcg tcgcgcccgt cagatcggcg tctcgccctt gattcaatgt ccggtttatc
     8581 cacttgcaga cattcgagga gttggtctgc tgaaccggcc caagcaagaa ggccgccgtg
     8641 acaggcggcc tccagacaag cgacgttcag atcgtcgggt tccacgctaa cggaatctcc
     8701 cggtaacccg tcccactctt ggttggttgc ataaccggca ggtcggcagg cttctcgccc
     8761 ttgaggatgc ggccgacata gataccgacc tgccgctcgc gctctgtgtt tcgcgacgcg
     8821 taacttatca aaccacccgc ctccacgaag gtgtccgctt tgagccagaa gcggacatta
     8881 gaggccatct ggatgagcga tgctgttgaa aaatgatttt gaggcggtct ctgagaaata
     8941 ttgattcaag accaaggtca aatacgcagt cttgattcaa aaagtctcgt cgccgcgtcg
     9001 aggtgcggta cgcctggtga tcgccgaccc aatggcgagc ccgcgaccaa cacgaaattt
     9061 ttttgctgcg aaagcgccgc acaagagttc catctggccg ccaagcggtc acattttcat
     9121 gtcagacgta aatttgctca gacgtaaatt ttgcgggaac tgtgctcgta aggggggcgc
     9181 gcatggcact cagcttacgc gatgttcggt gcgatccgat tgcgaaccgc gctctcaacg
     9241 agctcatgca ccattataca gtagcacaag agaaaagccg cttggttctg accaaaaaga
     9301 caggggacat gaagctcttc ttgaacgacc tcgatgacct tcatcaatgg gatttcatcc
     9361 gtaatcagca gatggtcaaa cagatcgaac gattgcgtgt gctattgacc tcgacaaacc
     9421 agcatcggga gagctggaag gtccgtgcct tgatagcaga ggcgcagtta ctcgaagcga
     9481 ctgtcaaaac cggcaacaag gggggtggtg agaaactcag tgacgtcagg tatgcctcgc
     9541 taaagcgcta tctggcaaag cggttccatc ctgattacgc ccccggacag ggcatcgaga
     9601 agattgttcg aaacgagatc ttcaaggaaa tctggaacga gattgaccgc ctcgatcaag
     9661 gagtctcggc ccgcggcgcg acagcgcgat cagctgcggc gacctgatcg ggcgattcgc
     9721 cgctccgcgg ccctgcaacg acgcgcgcgc tgcgcgaatg gtcggttccg gccagtgcca
     9781 ggccaagcat tgaatgtccg ctttaccccc gaaagcggac aaagagcaga catgtcggca
     9841 tgtccgcttt gtgccaaaag cggacattgc gcagtgcagc aaatcagcac cggtctcagc
     9901 agctttttgc agtctttggt tagctgtcgg cacccggcag gcgcaccgtg aacaccgacc
     9961 ctttgcctgg ctcgcttgtc acggtcacat cgccgcccat catgcgcgcg agcttgcgtg
    10021 acagagcaag gccgagcccg gtgccgccat agcggcgtgc ggtcagggaa tctgcctggg
    10081 tgaagtcctg aaacagctta gcctgctgtt cggcggtcaa gccgattccg gtatcggcaa
    10141 ccgcaagctc aatccagtcg cgcctgtcca ccaccttgcg cacccgaagg gcgacctcgc
    10201 cgtcttttgt gaacttacag gcattgctca gtagattgag cagaatctgc ttgagccgca
    10261 tggagtccgc ggtgagcgtg cccacgttct ccggcgcttc gacaacaagg cggttcttgt
    10321 tcttctcggt gagctgtccc gccgtgccga cgacttcatc gatcaggtgc gacagattaa
    10381 tagactcggg attaaggtcg agcttgccgg cctcgatctt cgaaaggtcc agcacctcgt
    10441 tgatcaagct caagaggtgt gtcccggcgg cattcacacg ccgcagcggc tcgagcgcct
    10501 tgtctgtgcc gaagcgcgcg gcattagtga ccatcatctc ggtcaggcct atgatggcgt
    10561 tgagcggcgt ccgcagctcg tggctcatgc tggagaggaa ctgcgacttg ttctcgctcg
    10621 cgacttcgag ttgttggctc ttgtcctcaa gctcagcggt gcgagcacgc agttcagtta
    10681 gcagccgcgt gttctcgatt gcgatgacag cctgagcggc gaaattcttg accagctcga
    10741 tttgcttgtc ggtgaaagga cgaacctcct gacgatagat gacaataacc ccgatcagct
    10801 catcatcttt gagcattgga acgccgagat aggtgcgcgc tcccgcaagc tttgtcgggg
    10861 cggttggaac gggtgaagca gcatcgtcga gggtgtggag cggtttcttt gacttccaca
    10921 cccggtaaag gccattcccg gcctccggga cgaacctccc cctccgccga aggaaatttg
    10981 tcagggccgg tggtgtattg accattgcca tggggtgaaa agcgtcgcca tcgaaccgga
    11041 atatggtccc aaattttgcg tcgcagatgc gcgtcgcgtt ctccagcatg gtttggaaca
    11101 ccggctccag ctccccgggc gagctggaga tgacgcgcag cacctccgag gccgccgtct
    11161 gctgttccag cgactcgctg agatcgtctg tgcgttggcg tagctcgttg agcaggcgcg
    11221 cgttctccat cgcgatcgcg gcttgcgcgg cgaagttttg gagcaaggcg atctgcttgt
    11281 cagtgaacgg ccgcacctcc tgacgataaa tcatgaaagt gccaagcaga gaacgatcct
    11341 tgcgcaacgg caagaatagt gcggtgcgag ctcctcccat ttcgtggagc gctcgcttgg
    11401 ctggattacc ggaccgataa ccctcatctt ccgtgatgtc cgggatgtgg acgaagtttt
    11461 ccccacggac cagttcatag gatgcgtttc cgggagccgg caccaacggc ttgttgtaga
    11521 acatcgtcaa aagtgccgga acacctcgca ttgcagtcgc gtaaatgtgc tcgccgtcat
    11581 aggtccagaa gccaccgaac gccgcctcgc acaggctcat ggccttttcc agcatcgtat
    11641 caaatactgg cgcaagctcg ccggctgaag aattaatagc cataagtacc tcagcggtgg
    11701 cggtttgctg ctccaatgac tgtgttaaat catctgtccg ctggcgcagc tcgttgagca
    11761 ggcgcgtgtt ctcgatagcg atgaccgctt gcgcggcgaa gttctggact agctcgatct
    11821 gcttgtcggt gaacggtcgc acctcctggc gatagatgat gatggcaccg acaagcctgt
    11881 tttccttaag cataggaacg ccaaggaatg tccgggcgcc ctcgagttcg accgtcgcaa
    11941 cacgtaacgg ttcgcgttcg gcataagcgg gttcggcagc gaggtccgtg atgtgaacga
    12001 cctgcttagt atcgactagc cgacgaatgc cacttcccgg gggcgggcac agtggcccgc
    12061 gctcccatag ctcgcgatag gccggagaaa catcgtgcag atatgcggca tgaacatgct
    12121 cgccatcata gagcagcaag tgaccaaatt tcgcttcgca gatgcgcatg gcgttcgcca
    12181 gcatggcttc gaatgcgggc ttcagctcac ccggcgacct tgagatgacg ctcagcacct
    12241 ccgaggccgc cgtctgctgc tctcgcgctt ccactagttc acgggtaaga gcgctgactc
    12301 gctcttgcag atcggcaacc gttgaactgg ctgggggcgc ggccgttcgc gcgttgctgc
    12361 gcgcccgctt ggccttgttg ccgtgccgcg ttttcgctgg tttacggctt atggtgccgc
    12421 gacgtcccac ctcgtgctcc ccgtgggcgg acgaacgcag cctaccacat catggggtcc
    12481 gttgtgcatc ccagcaaaat tcgctgtcga ttggccgcta tgggtcatta gcggacgtag
    12541 ggccacccct taggctatgt ccgctttggc cccgaaagcg gacattgagc gacacgactg
    12601 gcatgtctgc ttagtgccag aagcagtcgg cgtatcaatc ccggttattc gaggttcaat
    12661 gccccatgcc cggcatgact tggccattga aaatttcggg gttgggctga ccctcgacgc
    12721 tgagaatgcc gaccagcccg cgttccatac gggacagagc atgatcgacc agagtgtagt
    12781 tgccgggcac ctccagtttg aaatccacga tgacagcgcc gccggccggc accgtgaccg
    12841 tctggatgcc ttccagcggc gccgtctggg cgccgcccag catgtagacc ttgtcgaaga
    12901 tttcgccgat gacatggaaa gatgaggtgt aattcggccc gccgaccccg aagaaaatcc
    12961 gcaccgtctc accgaccttg gcgtggagtg gatgcagctt gctcagcgcg cccgccgagc
    13021 cgttcagcac gaaatactcc gggcgctcgt tcaaaagctt ctcgacgctg aactcggcgc
    13081 tgccgtgttg gccgtgcggg atatcggtgt agatttcgcc ctgcatcaca taaaactcgc
    13141 gatcgacggg cggcagaccg ccttcgggct cgacgaggat gagtccgtac atgccgttgg
    13201 cgatgtgttc ggcgaccatc ggcgtggcac agtggtagac ataaagtccc gg
//
LOCUS       DATLJT010000419         3308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_483748,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000419 DATLJT010000000
VERSION     DATLJT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483748"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VM910_09705"
     CDS             complement(<1..252)
                     /locus_tag="VM910_09705"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041961.1"
                     /note="catalyzes the formation of pyruvate and
                     beta-alanine from L-alanine and 3-oxopropanoate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HVI62842.1"
                     /translation="MLDKSPPAPKVNVPNDLAAHWMPFTANRAFKKQPRLLAGAKDMH
                     YFTVDGRKIIDAASGMWCTNAGHGRSQISQAIAKQAAELD"
     gene            complement(376..1281)
                     /gene="prmB"
                     /locus_tag="VM910_09710"
     CDS             complement(376..1281)
                     /gene="prmB"
                     /locus_tag="VM910_09710"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVI62843.1"
                     /translation="MVATGELLTLLDFVRFAVSRFVEAGLAFAHGTTDPVAEAAFLVC
                     EALHLHPDQFEAFAGARVTAAEGGTILDLIARRVTTRKPAAYLVNKVYMRGLPFYVDE
                     RVIVPRSYIGELLDSHFGGDDEGSSLIGVPAGVAQVLDLCTGSGCLAILAARHFPSAE
                     VDAVDISGDAIEVAARNVADYGLEGRVRLYRGDLFKPLGGKRYDLIISNPPYVDAGGM
                     AELPPECRAEPRLAFDGGADGLDLIRRILERAGRHLTAEGGLVCEVGRGREVLEAAFP
                     QLPLLWLDTEESQGEVFWIGAADLR"
     gene            complement(1359..1826)
                     /locus_tag="VM910_09715"
     CDS             complement(1359..1826)
                     /locus_tag="VM910_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HVI62844.1"
                     /translation="MTIPVTIRIQQGDFDVAKEIGALSEGRTDIGAVVSFSGICRGTE
                     GSREIAALTLEHYPGMAEAEIRRHAEEAMSRWPLQGLTIIHRFGRIEPGQNIVLVVTA
                     SSHRQAAFQAAEFLMDYLKTSAPFWKREESAKGASWVEAHVHDDAAAARWTKS"
     gene            complement(1844..2095)
                     /gene="moaD"
                     /locus_tag="VM910_09720"
     CDS             complement(1844..2095)
                     /gene="moaD"
                     /locus_tag="VM910_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HVI62845.1"
                     /translation="MKVKYFAWVRERIGKAEEMLEPPSSVRTVEDLIGWLAGRGDGYA
                     YAFETPRVIRAAIDHTHVRSDAAISGAREIAFFPPMTGG"
     gene            complement(2092..2706)
                     /gene="pgsA"
                     /locus_tag="VM910_09725"
     CDS             complement(2092..2706)
                     /gene="pgsA"
                     /locus_tag="VM910_09725"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HVI62846.1"
                     /translation="MNIATTRAQPKSLTLPNILTYARIAAIPVVVGCVYAQSIMDGPL
                     WLRWVAVAVFIAAGVTDYLDGYYARIWNQQSAFGRMLDPIADKLLVASCLLMLAADGI
                     IHGWSLWAAIVILCREILVSGLREYLAALRVSVPVTKLAKWKTTIQLVAIGFLLAGEA
                     GDMVVPVVTQIGLLLLWASALFTIYTGYDYFRAGIHHLIKEDEG"
     gene            complement(2778..>3308)
                     /locus_tag="VM910_09730"
     CDS             complement(2778..>3308)
                     /locus_tag="VM910_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVI62847.1"
                     /translation="LQRRFKRLLAPSETDKAKAEQDAVPQWPDLVIIDGGRGQLNAAR
                     EIFQGPDLKQVALMAVAKGPDRDAGRETLFLPDREAIKLEPRDPVLYFIQRMRDEAHR
                     FVIGSHRKRRTKDLREAGLQEIPGIGPSRKRALLHHFGTLKEIERASIADLGKVPGVS
                     AESARKIFEFFHAQTG"
BASE COUNT          592 a         1063 c         1060 g          593 t
ORIGIN      
        1 gtccagctcc gcggcctgct tggcgatcgc ctgcgaaatc tggctgcggc catggcccgc
       61 attggtgcac cacatgcccg acgccgcatc gatgatcttg cggccgtcga ccgtgaaata
      121 gtgcatgtcc ttggcgccgg cgagcagccg gggctgcttc ttgaaggccc ggttggcggt
      181 gaacggcatc cagtgcgcgg caagatcgtt cggcacattg actttggggg cgggggggct
      241 cttgtccagc atgggcgcct cttcgaggaa tcggtccggt tggctacccg cgaagctatc
      301 agcttcgcta ccccacggga acgtcaataa cttgcgatat tcccgctcat tcgatccgat
      361 tattgcgtag cacgcctacc ggagatcggc ggcgccgatc cagaacactt cgccctgcga
      421 ctcctcggtg tcgagccaga gcagaggcag ttgcggaaac gcagcttcca gaacctcgcg
      481 gccgcggccg acctcgcaaa cgagcccgcc ttccgccgtg aggtgccgtc cggcccgctc
      541 gaggatgcgc ctgatgaggt cgagcccgtc ggcaccgccg tcgaaggcga gtctcggctc
      601 cgcgcgacac tcgggcggca gttcggccat gccgccggca tcgacataag gtgggttcga
      661 gatgatcagg tcgtagcgct ttccgcccag cggcttgaag aggtcacccc gataaagtcg
      721 cacccggcct tcgaggccgt agtcggcgac attgcgcgcg gcgacctcga tcgcgtcgcc
      781 ggagatatcg accgcgtcga cctcggcgct cggaaaatgt ctggccgcga ggatcgccag
      841 acatccggag ccggtgcaga ggtcgagcac ctgcgcgacg ccggcgggaa cgccgatcag
      901 ggaggagcct tcgtcatcgc cgccgaaatg cgagtccagc aattcgccga tgtaggaccg
      961 cggcacgatc acgcgttcgt cgacatagaa gggtagcccg cgcatgtaga ccttgttgac
     1021 gagataggcc gctggcttcc gcgtcgtgac acggcgcgca atcaggtcga ggatcgttcc
     1081 gccctctgcg gcggtgacgc gcgcaccggc aaaagcctcg aactggtcgg ggtgcaggtg
     1141 cagggcttcg cataccagga acgccgcttc cgcgacgggg tcggtggtgc cgtgtgcgaa
     1201 ggcgagtccg gcctcgacga aacggctcac cgcaaaacgg acgaaatcga gcagcgtcag
     1261 caattcgccg gtggcaacca tcggaaatcg tgccgcgggc tttttgttcg caggcgtttt
     1321 tgccggcttg ttcgcagccg ccttgacgcg cttcgccatc aggacttggt ccagcgcgcg
     1381 gcggcggcat cgtcatgcac gtgcgcctcg acccagcttg ctcccttcgc gctctcttcc
     1441 cgtttccaga acggcgccga ggtcttcaga taatccatca agaactccgc cgcctgaaac
     1501 gccgcctggc ggtggctgga ggcggtcacg accagcacga tgttctgtcc gggctcgatg
     1561 cggccgaagc ggtggatgat ggtcaatccc tgcagcggcc agcgcgacat cgcttcctcg
     1621 gcgtgccgcc tgatctcggc ttccgccatg ccgggataat gctccagcgt cagtgccgcg
     1681 atctcccggc ttccctcggt gccgcggcaa atgccggaaa agctaacgac cgccccgata
     1741 tcggtgcggc cctcgctgag cgcgccgatc tctttggcga catcgaaatc gccttgctgg
     1801 atccggatgg tgacggggat cgtcatgcgc ggaccggcgg tgttcacccg ccggtcatcg
     1861 gcggaaaaaa ggcgatctcg cgcgcgccgg agattgccgc atccgacctg acatgggtgt
     1921 gatcgatcgc ggcgcggatt acccgcgggg tctcgaaggc ataggcgtag ccgtcaccgc
     1981 gcccggccag ccagccgatc aggtcttcga cggtgcggac gctcgacgga ggctccagca
     2041 tctcctccgc cttgccgatg cgctcacgca cccaggcgaa atacttcacc ttcatccttc
     2101 atcctccttg atgagatgat ggatgccggc gcggaaatag tcgtagccgg tgtagatcgt
     2161 gaacagggcg gaggcccaca acaggagcag gccgatctgg gtgacgaccg gcaccaccat
     2221 atcgccggcc tcgcccgcaa gcaggaagcc gatcgcaacc aactggatcg tggtcttcca
     2281 tttggcgagt ttcgtgaccg gcacgctgac acgaagcgcg gcgagatatt cgcgcaagcc
     2341 cgacaccaga atttcacggc acaggatgac gatcgcagcc cacagcgacc agccgtgaat
     2401 gataccgtcc gcggcgagca tcaacaggca ggaggcaacc aggagcttgt cggcgatcgg
     2461 atcgagcatg cggccgaaag ccgattgctg gttccagata cgcgcgtagt agccgtcgag
     2521 gtagtcggtg acgccggcag cgataaagac agcgaccgcc acccagcgca gccaaagcgg
     2581 accgtccatg atcgactggg cgtagacgca gcccaccacc accgggatag cggcgatcct
     2641 ggcgtacgtc aggatattgg gcagcgtcag gcttttgggc tgcgcccttg tggttgcgat
     2701 gttcattagc tctaccaata ccgccccaat gtgaaggtca atcgactaaa ctggaagtca
     2761 cgtttccgga ccatgcttta gcccgtctgg gcgtggaaaa attcgaaaat cttgcgcgcg
     2821 ctctcggcac ttacgcctgg aactttgccg aggtcggcaa ttgaggcccg ttcgatctcc
     2881 ttcaaggttc cgaaatgatg cagcaaggca cgtttgcgtg acggtccgat gccggggatt
     2941 tcctgtaaac cggcctctcg caggtctttg gtgcgccgct tgcggtgcga gccgatcacg
     3001 aagcggtgcg cctcgtcgcg catccgctgg atgaaataca gcacgggatc gcggggctcg
     3061 agcttgatcg cctcgcggtc cggcaggaac agggtttcgc gcccggcatc gcggtccggc
     3121 cccttggcga ccgccatcag cgcgacctgt ttcaagtccg ggccctgaaa gatctcgcgc
     3181 gccgcattga gctggcctcg gccgccgtcg atgatcacaa ggtcgggcca ttgcgggacc
     3241 gcatcctgct cggctttggc cttgtcggtt tccgacggcg ccagcaggcg cttgaagcgc
     3301 cgctgcag
//
LOCUS       DATLJT010000420         3542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3929813,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000420 DATLJT010000000
VERSION     DATLJT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929813"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VM910_09735"
     CDS             complement(<1..237)
                     /locus_tag="VM910_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62848.1"
                     /translation="MKLPRRTFLHLAAGAAALPAVSRVARAQAYPARPVRWIVPYPPG
                     GAADPIARLIGPFLSERLGKPVVIENKPGAGANIG"
     gene            complement(262..1248)
                     /locus_tag="VM910_09740"
     CDS             complement(262..1248)
                     /locus_tag="VM910_09740"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62849.1"
                     /translation="MTRREFITLIGGTAAAWPLAARAQGDRIRRLGVLMSTGERDPET
                     QLRVGAFREGLQKLGWAEGRNLQIDYRWGAGSIERTRTYAAELVALKPDVILGAPASA
                     AVALHRETRTIPVVFAQVPDPVGLGMVESLSRPGGNITGFALYEYAIALKWLELLKEI
                     APHVTRAGILYDLEQPTSPAYLKTIEATAPSFKVQILPDAVRDAGTIERAIATLASGT
                     NSGLLVLPGALMTRHRDLIISLAARHRLPSISAFRYYVAGGGLASYGVDNLDLYRRAA
                     GYVDRVLKGEMPANLPVQFADKFELVINVKTAKTLGLEVPVSLLARTDEVIE"
     gene            complement(1245..2225)
                     /locus_tag="VM910_09745"
     CDS             complement(1245..2225)
                     /locus_tag="VM910_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62850.1"
                     /translation="MTRRAFITLIGGAVAWPLAARAQQDVTPTVGFLNTLGQNDRPNL
                     PAAFRRGLSETGRVDGRNVTIEYRFAENQHDRLPALAADLVGRKVAVIAATGGGASIL
                     AAIAATKSIPIVFTTGGDPVKEGYVASLNRPGGNVTGVSWFSNLVAGKALGLLYELVP
                     NAALIAVLTNPKLPESARTPSDAQEAARTLGQQLLLLDASTPSEIDAAFTTMRQRRVG
                     ALFVGADPFLSSRRQQIVALAARDAIPTMYTNHEFVEEGGLISYGNDTANGYVRAGVY
                     AGRILNGAKPSDLPVDQATKFELVVNVKAAKAIGLTIPESFLARADEVIE"
     gene            complement(2222..3217)
                     /locus_tag="VM910_09750"
     CDS             complement(2222..3217)
                     /locus_tag="VM910_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62851.1"
                     /translation="MTVTIGRRELLAALGGAVAAWPRAARAQQPALPVVGFIRTTSAD
                     DSLKLVEAFRRGLGEAGYVEGRNVLIEYRYAQGQIDRLPALAADLVTRRVAVLAATGG
                     TVSARAAKAATSTIPVVFTTGDDPVKAGLVASLSRPGGNVTGVSVFTARLGAKRLALL
                     HELIPAATTIAILLNPKNPDSEDEAKDVQEAARALGVEILVLYAGTENEIDAAFTKLV
                     EQRTGALMLGADTFFTSQRARIATLSTYLRIPTADSVREFPEAGGFASYGASLAGVYR
                     QAGVYVGRILKGDKPADLPVLLPTAFEFVINLQTAKVIGLTVPDKLLVAADEVIE"
BASE COUNT          640 a         1094 c         1151 g          657 t
ORIGIN      
        1 accgatattg gcgcctgctc caggcttgtt ttcgatcacg accggcttgc ccaggcgttc
       61 cgatagaaac gggccgatga gtcgcgcgat cgggtcggct gcgcctccgg gtggataggg
      121 aacgatccag cgcaccggcc gcgccggata ggcttgcgcc ctcgcgacgc gcgatacggc
      181 cgggagcgcg gcagcgcctg cggccagatg cagaaatgta cggcggggaa gtttcattct
      241 ggacctccct ttcgcttcac atcattcgat cacctcgtcg gtgcgcgcga gcagcgagac
      301 tggcacctcc aggccaagcg tcttcgctgt cttaacgttg atgaccagct cgaacttgtc
      361 cgcgaactgc accggcaagt tggccggcat ttcgcctttc aacacgcggt cgacatagcc
      421 tgccgcgcgc cggtagagat cgagattgtc cacgccgtag gacgcaagcc caccacccgc
      481 gacgtagtag cgaaacgcgg agatactcgg caatcgatga cgcgctgcca gcgagatgat
      541 gaggtcgcga tggcgcgtca taagcgctcc cggcagcact aggaggccgc tatttgtccc
      601 tgacgcgagt gtggcgatgg cgcgctcgat ggttcctgcg tcgcgcactg catccggcag
      661 gatttggacc ttgaatgatg gcgcagtcgc ttcaatcgtc ttgagatatg cgggcgaagt
      721 cggctgctcg aggtcgtaca ggattccggc tcgggtaacg tgtggtgcaa tctccttaag
      781 cagctccagc catttcaacg caattgcgta ttcatagagg gcaaagccgg tgatgttgcc
      841 gcccggccgc gacaggcttt ccaccatgcc gaggccaact ggatcgggca cctgcgcgaa
      901 cactaccggg atggttcgcg tctcccgatg caatgccacg gccgcggatg cgggagcgcc
      961 aaggatcacg tccggcttca gcgccacgag ttccgccgcg taggtgcgcg ttcgctcgat
     1021 gcttcccgct ccccaacgat agtcgatttg aagattgcgg ccttccgccc agccaagctt
     1081 ctggagtcct tcgcggaacg ctcctacccg gagctgagtt tcgggatcac gttcgccggt
     1141 cgacatcaac acgccgaggc gccggatgcg atcaccttgc gcccgcgccg cgagcggcca
     1201 cgcggccgcc gtgccgccga tgagcgtgat gaactcgcgg cgcgtcattc gatcacctcg
     1261 tcggcacggg cgagaaagga ttcaggaatc gtaaggccga tcgctttcgc ggctttgacg
     1321 ttgacaacga gttcgaactt ggtggcttga tcgaccggca ggtcggaggg cttggcgcca
     1381 ttgagaatgc gtcccgcgta gactcctgcg cggacatatc catttgcggt atcgttgcca
     1441 taactgatca gcccgccctc ttcgacaaat tcgtggttgg tgtacatggt cgggatggcg
     1501 tcgcgcgccg ccagcgcaac aatttgctgc cgacggctgg ataagaacgg atcggcaccg
     1561 acaaataacg caccgacgcg cctttggcgc atggtggtaa acgcagcgtc gatctcgctt
     1621 ggggtgctgg cgtcgagaag gagcaactgc tggccgaggg tgcgcgcggc ctcctgcgcg
     1681 tcgctcgggg tgcgcgcgct ttctggaagc ttcggattgg tgagcacggc aatcagcgca
     1741 gcattgggaa cgagttcgta cagcaatccc agcgccttgc cggcgaccaa gttactgaac
     1801 cagctcactc cggtgacatt gccgcccggc cggttgaggc tggcgacgta gccttccttg
     1861 accgggtcac cgccggtcgt aaacacgatg ggaatgctct tagtagccgc gatggcagcc
     1921 aatattgaag cgccacctcc tgtcgcggcg atcacggcca ccttacggcc gaccagatcg
     1981 gccgcaagag ccggcaatcg atcatgctgg ttttctgcaa aacggtattc aattgttacg
     2041 ttgcgcccgt cgacacggcc ggtttcactc aagccacggc ggaacgctgc cggaaggttg
     2101 ggtcggtcat tttgccctag ggtgttcagg aacccgaccg tgggcgtgac gtcctgctgc
     2161 gcgcgtgccg cgagcggcca cgccaccgcg ccgccgatga gcgtgatgaa cgcgcggcgc
     2221 gtcactcgat cacctcgtcg gcggcgacaa gcagcttgtc cggtacggtc aggccgatca
     2281 ccttggcggt ttgcaggttg atgacgaact cgaacgcggt gggcagcagg acgggcaggt
     2341 cggccggctt gtcgcccttg agaatgcgcc caacgtagac gccagcctga cgatagacgc
     2401 cggcgaggct cgctccgtaa ctggcaaaac cgcccgcctc gggaaactcg cgcaccgagt
     2461 cggcggtagg aatgcgcagg tacgtcgaca gcgtggcaat gcgcgcgcgt tggctggtga
     2521 aaaaggtgtc ggcgccgagc atcagcgcgc cggtccgttg ttcgactagc ttggtgaagg
     2581 cggcatcgat ttcattctcg gtcccggcat acagaaccag aatttcaacg ccgagcgcgc
     2641 gcgcagcttc ctgcacgtcc ttcgcctcgt cctcgctgtc gggatttttc ggattgagca
     2701 ggatcgcaat cgtggtggcg gcggggatca gctcgtgcag caaggcgagc cgctttgctc
     2761 cgaggcgcgc ggtgaacacg ctgacaccgg tgacgtttcc gcccggacgg ctgaggctgg
     2821 caaccagacc ggccttgacc ggatcgtcgc cggtcgtgaa gacgacgggg attgtgctgg
     2881 tcgccgcctt ggcagcacga gccgaaacgg tgccgccggt cgccgctagc accgcaacac
     2941 gccgggtcac cagatcggcg gcgagcgccg gaaggcggtc gatctggccc tgcgcgtagc
     3001 gatattcaat caggacgttg cggccttcga catagccggc ctcgcccaag ccgcgccgga
     3061 atgcctccac cagcttcaac gagtcgtccg cagacgtagt cctgatgaac ccgaccaccg
     3121 ggagcgccgg ctgctgcgca cgcgccgcgc gcggccacgc cgctaccgcg ccgccgagtg
     3181 cggccagcaa ttcccgccgt ccgatggtga ctgtcatgcc gcccctccgt gcgagggtgc
     3241 gtagaggaaa cgataccacg tcacgagcgt gcagtcttgc agttcaagga gggcaggatg
     3301 ctcgccatag ccgctgtcta tcgtcgggcc gctctcatac gtgctgcggt atcaagtgtc
     3361 gcttcgcgat tcgtttgtcg gggaaaaagg gcgttgaggc gccattcgcc cgttcgagaa
     3421 gaagccgcgt aggttcgcgg ttggtgcgtt agtcagggtg accaagggac cgttcgaggg
     3481 aatggttgga actgtcgagg tcgcgatcaa acggtccaca aaagtagtaa acgattttgg
     3541 ga
//
LOCUS       DATLJT010000421         2114 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1173329,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000421 DATLJT010000000
VERSION     DATLJT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173329"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..138)
                     /locus_tag="VM910_09755"
     tRNA            complement(63..138)
                     /locus_tag="VM910_09755"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Trp,seq:cca)
     gene            complement(208..1377)
                     /locus_tag="VM910_09760"
     CDS             complement(208..1377)
                     /locus_tag="VM910_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI62852.1"
                     /translation="MKGHIKQRSAGHFAIVLDTRDPQTGKRRRKWISFSGTLRGARIE
                     CARLVTEMKSGAYVDATRETIAAFLDRWIEHMQGQVSPRSLERYAELCRNNIAPLLGA
                     IPLTKLQPANISQAYAKALASGRRDGTGGLSPLTVHHMHRVLREALQQAVRWQLLARN
                     PVDAVRPPKVERKQMQTLDAEATVVLIEAARGTNLFMPILLGVLCGLRRGEITALRWR
                     SIDLDRGQLSVTASTEQTNRGIREKETKSGKSRTVALPSLIVDELRRHRVAQAEGLLA
                     AGVRLTDDHHVVAREDGQPLQPRSLTHAFVKFVRGHGLAPIRLHDLRHSHATHMLASG
                     VHPKIAQERLGHSSVSVTIDLYSHVLPGMQAEAVSRVDAVLRDALNRRAVPTKKW"
     gene            complement(1374..1913)
                     /locus_tag="VM910_09765"
     CDS             complement(1374..1913)
                     /locus_tag="VM910_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62853.1"
                     /translation="MAKRGRPFGGEKGSVLSVRVSPETKRELDYFARKYRRKLSREVE
                     DRLKFSFDRYGRPPHIADLADLVALLAQLVERKTRRQWDRHDATRESLIRAFTRLVDV
                     YSKADVTSSSISVPGSKADFERGRDDPAMNAVSEVILSLLHSVHLGQGPGVYDLGVGV
                     LSKIQRNLERNSERRRRKK"
     gene            2052..>2114
                     /locus_tag="VM910_09770"
     CDS             2052..>2114
                     /locus_tag="VM910_09770"
                     /inference="COORDINATES: protein motif:HMM:NF020291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62854.1"
                     /translation="MTGHLLRRYVDPRVTLELIRT"
BASE COUNT          386 a          658 c          632 g          438 t
ORIGIN      
        1 tcggggcgcg gcccacaacc cattgcgctg tctagacttt gctgaccgag ccggactacg
       61 cctggcagga gtggagggac tcgaaccccc aacccccggt tttggagacc ggtgctctag
      121 ccattgagct acactcctaa gtgcttttct cgccttaagt ctttcctccg atgtcgtccc
      181 caaacagcac cgtttgctac cgctttgcta ccattttttg gtgggaacag cgcggcggtt
      241 tagcgcgtcg cgaagcacag cgtcaactcg gctgactgct tcggcctgca ttccgggaag
      301 aacgtggctg tagagatcga tcgtcacgct cacgctcgaa tgtccaagcc gctcctgcgc
      361 gatcttggga tgcactccgg atgccaacat gtgggtggcg tggctgtggc gcaagtcgtg
      421 cagccggatc ggcgccaggc catgccctcg cacgaatttg acgaaggcat gggtgagcga
      481 ccgcggctgt agcggttgtc catcctcgcg ggccacgacg tggtgatcgt cagtgagccg
      541 cacgccggcc gccagcagcc cctcagcctg tgcaacccgg tgccggcgca gttcgtcaac
      601 gatcagcgac ggcagggcca cagtgcggga cttgccgctc ttggtctcct tctccctgat
      661 gccgcgattg gtttgttcgg tactcgcggt cacggacagc tgaccgcgat ctagatcgat
      721 cgagcgccag cgcagcgccg tgatctcgcc ccggcgcagg ccgcacagga cgccgaggag
      781 gatcggcatg aacaggttcg tgccgcgcgc cgcctcgatc agcaccaccg tcgcctccgc
      841 gtcgagggtc tgcatctgct tgcgttcgac tttggggggc ctgacggcgt cgaccggatt
      901 gcgagcgagc aactgccaac gcacggcctg ctgtagggct tcccgcagga cgcggtgcat
      961 gtgatgcaca gtgagtgggg ataggcctcc agtgccatcc cggcggccgc tggcaagcgc
     1021 tttggcgtaa gcctgggaga tattggccgg ctgtagcttt gtcagcggta tggcccccag
     1081 caacggcgcg atgttgttgc gacagagttc ggcgtatcgc tcaagggagc ggggtgagac
     1141 ttgcccctgc atgtgttcga tccagcggtc gaggaaggcg gcgatcgtct cacgcgtcgc
     1201 atcgacatag gcgccgcttt tcatttcggt gacgaggcgg gcacattcaa ttcgtgcccc
     1261 ccttaatgtt cctgaaaatg aaatccactt gcgtcgacgc ttgccggtct gcggatcgcg
     1321 cgtgtcgagg acgatcgcaa aatgcccggc tgagcgctgt ttgatatggc ctttcatttc
     1381 ttgcgcctcc ttctctcaga gttgcgttca aggttgcgtt gaattttcga aagaacaccg
     1441 acgccgagat cgtacactcc cggcccctgt cccaaatgaa ccgaatgcag caaagacaag
     1501 atcacttcgc tgaccgcgtt catcgcgggg tcatcgcgtc cacgttcaaa atctgctttg
     1561 gagccaggga cgcttatcga cgacgaagtg acatccgcct tgctgtacac atcgaccagg
     1621 cgggtgaaag ccctgatcag ggattcacgg gtggcgtcgt gacgatccca ttgcctccgc
     1681 gttttccgct caaccaattg cgccagtaac gcaacaaggt ccgccaagtc cgcgatatgg
     1741 ggcggccggc cataccggtc gaacgaaaat ttgaggcgat cttctacctc gcgagataac
     1801 ttgcgccgat atttccgcgc gaagtaatcc agctcccgtt tcgtctcggg agaaacgcgg
     1861 accgagagca ccgacccctt ttccccgccg aacggtcgac cgcgcttcgc catggcccgc
     1921 taaattgttg tatgacaaaa gtattacctc aaccttgatc acctcgtcaa gtcccacggc
     1981 tccgtccgag tggcgccggt tagtgatcgt acggcgtcgc cgaaggcgag cgcaacgaga
     2041 gcatcgcgag gctgactggc catttgctgc gccgttatgt cgatccccga gtcacgctcg
     2101 aattgatccg cacc
//
LOCUS       DATLJT010000422         3957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3929904,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000422 DATLJT010000000
VERSION     DATLJT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3929904"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1098)
                     /locus_tag="VM910_09775"
     CDS             complement(55..1098)
                     /locus_tag="VM910_09775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62855.1"
                     /translation="MNETRTIASALVAATLAFAPQAAWSQQPAPLLEFNYGIPTADHA
                     TVFVAQDLNLFEKAGLKPKFFYFQSGAPLLAGLKSESLDVVTAGLALTFALGQDIPLK
                     FLFWEANNAAAEGLVVDPNGPVKSYQDIGKAQKIGAASGTCAQVALYLMAKKVGVDYA
                     KLNVVNIAAPLFRNSFLSHSIDAGISWPPYSLQLQSEGFPVANFDEDYLEPGGVCPGL
                     TGVRPAFVAAHPEIAERLIAVEAMAREEMRKNPQVGVDAFVKHLSVTPEVAKATLDRE
                     CCGRVPSFADQLDPASPFSMTSKDHGLVGKLFLASEVLAATKAIPQPIPLEKIQAAVD
                     PTYLQKYMNSHPN"
     gene            complement(1151..1981)
                     /locus_tag="VM910_09780"
     CDS             complement(1151..1981)
                     /locus_tag="VM910_09780"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62856.1"
                     /translation="MPVPPQSTQPTTARQPKISFQGAQKLYAGKGGSTLALDSFSLDI
                     MPTEVISIVGPTGCGKSTALNLLAGFETPSSGAVAVDGKVVNGPGSDRGVVFQQPSLF
                     PWFSVLDNVVLGVKSRGVPKADYGPRAINLLAEVGLKGFENHYPYQLSGGMQQRVQIA
                     RGLIGEPDILLMDEPFGALDYQTRLLMQKLLLRLWEHFKPTILFITHDVGEAVFISDR
                     VIAMTRRPGRVKRDVRVTAPKPRDYEFLGSPEFTELEHELVLAVQSEVDPDEVKNKAA
                     "
     gene            2503..3399
                     /locus_tag="VM910_09785"
     CDS             2503..3399
                     /locus_tag="VM910_09785"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI62857.1"
                     /translation="MNRYRRIGDQIDILGESPIWHEREGALYWVDVRRPAIRRLDHAN
                     GNVVTWNMPGLIGSIAFAENGRLLAALADQIALFEPATGKLEAVASLPARIPDHRFND
                     GRCDRQGRFWVGTMHNITRAPEGVLYRLDPERGELLTAKTGICIPNSLAWSPDGRTMY
                     FADSLRYAIFAYDFDTASGQMSNERTFVETRAPGFPDGSTVDADGYLWNAEFNAWRVV
                     RYAPDGRVDRVIELPAERPTSCAFGGPNFDVLYVTTASQWMSESEMAAQPMAGALLAI
                     ETDVRGLREPRLATKTAIQKHS"
     gene            3413..>3957
                     /locus_tag="VM910_09790"
     CDS             3413..>3957
                     /locus_tag="VM910_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI62858.1"
                     /translation="MDPIKLSSIETFAFRVPTKPIKVSFGTFRDRPCVLVRVVDVDGH
                     EGWGEVWCNWPAVGAEHRARLAADIGERLIGRTLASPDEAFHSLTKEMEVLVLQTGES
                     GPIAAAIAGIDIAIWDLSARKKGVPLYRHLGGPDTKFVPVYATGINPDQPEEFAVARY
                     REGHRAFKLKIGFGREIDVRNL"
BASE COUNT          775 a         1180 c         1215 g          787 t
ORIGIN      
        1 gggggcaaag ctggtcgcga cgccttcgat ttggatgttg gacatggctc gagctcagtt
       61 tgggtgggag ttcatgtatt tttgaagata ggtcggatcg accgccgcct ggatcttctc
      121 caggggaatc ggctggggaa tcgccttggt tgcggcaagc acctcgcttg cgagaaacag
      181 cttcccgacc agaccatgat ccttcgacgt catcgagaaa ggcgatgccg gatcgagctg
      241 atcggcaaag ctcggcacgc ggccgcagca ctcgcgatcg agagtcgcct tcgcgacttc
      301 gggcgtcacc gaaagatgct tgacgaaggc gtccacgccg acctgcggat ttttccgcat
      361 ctcttctcgc gccattgctt cgacggcaat gagccgctcc gcgatctcgg gatgagccgc
      421 gacgaacgct ggccgtacgc cggtcaatcc cgggcagacg ccgcccggtt cgagatagtc
      481 ttcgtcgaaa ttggcgaccg gaaagccttc cgactgcaac tgtagcgaat agggcggcca
      541 cgagatgccg gcatcgatcg aatggctgag gaacgagttg cgaaaaagcg gcgccgcaat
      601 attgacgacg ttgagcttgg cgtaatcgac gccgaccttc ttcgccatga gatagagggc
      661 gacctgggcg caagtacccg aagctgcgcc gatcttctgg gccttgccga tgtcctgata
      721 cgactttacg ggaccgttgg gatcgaccac caagccctcg gcggcggcat tattggcttc
      781 ccagaacaga aacttgagcg gaatgtcctg gccgagcgcg aacgtcagcg cgagccccgc
      841 ggtaacgacg tccaggcttt cgctcttgag accggcaagc agcggcgcac cggattgaaa
      901 atagaagaac ttgggcttca gtccggcctt ctcgaacaga ttgaggtcct gcgcgacgaa
      961 gaccgtcgca tgatcggcgg taggaatgcc gtagttgaac tcaagaagcg gtgcgggctg
     1021 ttgcgaccaa gcggcttgcg gtgcgaacgc cagcgtcgca gcaactagcg cagacgcaat
     1081 ggtcctcgtt tcgttcatcc cttcctccct gaactttctt tttttgccac cgcgaggcca
     1141 ccagcgtccg ctacgcggcc ttgttcttca cttcatccgg atcgacttcg ctctggaccg
     1201 ccaggaccag ctcatgctcg agctcggtga attccggcga acccaggaat tcgtagtcgc
     1261 gtggcttggg tgcagtgacc cgcacatcac gtttcacccg tccgggacga cgcgtcatcg
     1321 cgatgacgcg atccgagatg aaaaccgctt cgccgacatc gtgcgtaatg aagagaatcg
     1381 tgggcttgaa gtgctcccac aggcggagca ggagcttctg catcagcagc cgcgtctgat
     1441 agtccagcgc gccgaatggt tcatccatga gcagaatgtc gggctcgccg atcagcccgc
     1501 gggcaatctg gacccgctgc tgcatgccgc cggaaagctg atagggatag tggttctcga
     1561 agcctttcag gcccacttca gcaagcaaat tgatggcgcg cggcccgtag tcggcctttg
     1621 gcaccccgcg gctcttcacc ccgagaacga cattgtctag caccgagaac cacggaaaca
     1681 acgatggttg ctggaacacg acgccacggt ccgatcccgg accgttgaca accttgccgt
     1741 cgaccgcgac ggcgcccgat gacggcgtct cgaaaccggc gagcaggttg agcgccgtcg
     1801 acttcccgca gccggtcggg ccgacgatcg agatgacttc cgttggcatg atgtcgaggc
     1861 tgaaggagtc gagggccagc gtgctcccac ccttacccgc gtagagcttt tgcgcgccct
     1921 ggaacgagat cttcggctgc ctggcggttg tcggctgtgt ggactgcggt gggactggca
     1981 tggctgctcc tgacatcgcg gcgtgtcgtg gatcgattgc cattttggcc atcgacgtta
     2041 gtggtggacc agatcgacct ccagcctgag cggcgcagat ccgatcgaag tttgccgtgc
     2101 aaaaaacctg tatcgctctt tcgctcttgt cgcttctctc cagggccgga agcggtatct
     2161 cgataaatct cggtgttcgc aaaccgaaaa tgcgacggcg tcaatgtgcc ccgaagcggc
     2221 gtggaacgaa gctgcaatcc aatcgacctt gcgccggcgg atcaccttcg gctcctctcc
     2281 cgtggatttt tttgtccggc gttcgcgatg tcttcgcatc tgcgaactcc gattgctaca
     2341 atctcgcgat tatcataatc ctaatatccg acggctgggt gctcaactac aatctcccga
     2401 tcgctagaca catcagatgt cgatgcacca gcaacgagga gccgccgcga attacgaacg
     2461 cgcggcttcg atcgtcgcga catctcttca tcgagactgg ccatgaacag gtatcgccgc
     2521 atcggggatc agattgacat tctgggcgag agcccgatct ggcacgagcg tgaaggggcg
     2581 ttgtactggg tggatgtgcg tcggccggcg atccggcgcc tggaccacgc taacggcaac
     2641 gtcgtcacct ggaacatgcc gggcctgatc ggctccatcg cgttcgcgga gaatggtcgt
     2701 ctcttggctg cgctggccga ccagattgca ctgttcgagc ccgcgacggg aaagctcgag
     2761 gccgtagcga gcttgcccgc gcggatcccc gaccatcgct tcaatgacgg tcgatgcgat
     2821 cggcaaggtc gcttttgggt gggcacgatg cacaacatca cgcgcgctcc ggaaggcgtt
     2881 ctgtaccggc tcgatcccga acgcggcgaa ctcctcacag cgaagaccgg aatttgcatc
     2941 cccaacagcc tggcatggag ccccgacggg cgcaccatgt atttcgcgga ctcactccga
     3001 tacgcgattt tcgcctacga tttcgatact gcatccgggc agatgagcaa cgagagaacg
     3061 tttgtcgaga cgcgcgcccc gggttttccg gacggctcga cggttgatgc cgacggctat
     3121 ctgtggaacg ccgaattcaa tgcctggcgc gtcgtgcgct atgcgccgga cggccgcgtc
     3181 gatcgcgtga tcgagctgcc tgccgagcgg cccacatcat gcgcgttcgg cggcccgaat
     3241 ttcgatgttc tatacgtgac gaccgcaagt cagtggatga gcgaaagcga aatggcggcg
     3301 caaccgatgg cgggtgcgct actcgccatc gaaactgacg tccgtgggtt gcgcgagcct
     3361 cgtcttgcga caaagaccgc aattcaaaag cattcatgaa tggagcccga agatggatcc
     3421 gatcaagctt tcgtcaatcg agaccttcgc tttccgagta ccgacgaagc cgatcaaggt
     3481 ctcgttcgga acttttcgcg accggccgtg cgtcctcgtt cgtgtcgtcg atgtggacgg
     3541 tcatgaaggc tggggcgaag tgtggtgcaa ctggccggcg gtcggggccg agcatcgtgc
     3601 gcggctggcg gcagacatcg gcgaacgact gatcggtcgt acattggcat cacccgacga
     3661 agcctttcac agcctgacga aagaaatgga ggttctggtc ctgcagactg gcgagagcgg
     3721 gccgatcgct gcggcgattg cgggcatcga catcgccatt tgggatcttt ccgcccgcaa
     3781 gaagggcgtc ccgctttatc gtcatctcgg gggtccagac accaaattcg tcccggtcta
     3841 cgcgaccggc ataaacccgg atcaacccga ggagtttgcc gtggcgcgtt accgcgaggg
     3901 gcaccgggcc ttcaagctga agattggctt tgggcgggaa atcgacgtca ggaatct
//
LOCUS       DATLJT010000423         2237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2781906,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000423 DATLJT010000000
VERSION     DATLJT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2781906"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /locus_tag="VM910_09795"
     CDS             complement(<1..1304)
                     /locus_tag="VM910_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62859.1"
                     /translation="MRSFATIVVIALGYLFSAVEGSGQTAVVVGSSCVVVNQNVKATD
                     GGIVVNQLECPESPENSFILRYVWLDRTASSLMLVSQFDEALKPLVGDVQTVIRNPVF
                     DQVDTLVQKFGAPPVNVPGTGGMFDKGMRYNIMGKSDAEVQSAGTPMPLKKLKTFKLY
                     DAETTILLPDVDGLREVLKTRGWPRNYGLTYSTENVNLNFKNKKPENENLAISCVLLH
                     RPVPKSELENYWPAMENLERAMDAREFRPSEVISNEVGGTDFRVAAVQNRAIDAMLYF
                     GEKSWPDDFLMSFGNAVSDQCGDGYSFGYFAPPRSLYTLVAVVEAKNNDIEVQQIRYL
                     VDDEAGLRKLRKGTRDDQSPVGVVNLKQGESLLIPLRIELRYDMEVWPMSALNSQPAA
                     EDVRQKILEAPMSVLRFQGKDDPGDNKAPARVVFEKLRSEFRP"
     gene            complement(1517..>2237)
                     /locus_tag="VM910_09800"
     CDS             complement(1517..>2237)
                     /locus_tag="VM910_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI62860.1"
                     /translation="YAAGGYYYPGKDLSMLRSEMRGYLDRGYNVVKMKIGGAPIAEDR
                     ERIEAVLKEIGKDAQLAVDANGRFDLETAIAYAKMLRDYPLFWYEEAGDPLDYALQAA
                     LAEFYPAPMATGENLFSHQDARNLIRYGGMRPDRDWLQFDCALSYGLCEYQRTLAVLH
                     AHGWSPSRCIPHGGHQMSLNIAAGLGLGGNESYPDLFQPYGGFPDGVRVESGHIVMPE
                     LPGIGFEGKSDLHAEMKRLAA"
BASE COUNT          427 a          683 c          654 g          473 t
ORIGIN      
        1 gggcgaaatt ccgagcgcag cttttcgaag acgacccgcg ccggagcctt gttgtcgccg
       61 ggatcgtcct tgccctggaa gcgaagcacg ctcatcggcg cctcgagaat tttctgacgt
      121 acatcctcgg cggccggctg cgagttgaga gccgacatcg gccacacctc catatcgtaa
      181 cgcaactcga tccgaagcgg gatcaacagc gactcgcctt gcttcaggtt cacgacgccg
      241 accggcgatt ggtcatccct ggtacccttc ctcagctttc gcaggccggc ctcatcgtcg
      301 accagatatc tgatctgctg cacctcgatg tcgttgttct tggcctcgac gaccgcgaca
      361 agcgtataga gacttcgcgg cggggcgaaa tagccgaagc tgtaaccgtc cccgcactgg
      421 tcggacaccg cattgccgaa agacataaga aaatcgtccg gccaggattt ttcgccgaaa
      481 tacagcatcg catctatggc gcggttctgg actgccgcga ccctgaaatc cgtgccgccg
      541 acttcgttgc tgatgacttc gcttggccgg aactccctgg cgtccattgc cctctcgagg
      601 ttttccatcg ccggccaata attttcgagc tccgattttg gcaccggccg gtgcagcaac
      661 acgcacgaga tggcgaggtt ttcgttttcc ggtttcttgt tcttgaaatt gaggttcacg
      721 ttttccgtcg aataggtcag cccgtaattc ctgggccaac cgcgtgtctt caagacctcc
      781 cggagaccat cgacgtcagg caacaggatt gttgtctcgg cgtcatagag cttgaacgtt
      841 ttcagctttt tgagaggcat gggcgtgccg gcgctttgca cttcggcgtc cgactttccc
      901 atgatgttgt agcgcatccc cttgtcgaac atgccgccgg ttccgggcac attgactggt
      961 ggcgctccga acttctgaac caaagtgtcg acctggtcaa agacggggtt gcgaatgacg
     1021 gtttgcacgt cgccgaccag cggcttaagc gcttcgtcga actgactgac gagcatgagg
     1081 gatgaggcgg tgcgatccag ccagacatat ctgagaatga agctgttttc gggagattca
     1141 gggcactcaa gctggttgac gacgatgccg ccgtcggtgg ccttgacgtt ctggttcacc
     1201 acaacgcagc ttgatccaac gaccacagcc gtctggcccg agccctcgac cgccgagaac
     1261 aaatatccca gggcgataac gacgatcgta gcgaagctgc gcatcgatgt gctccctgcc
     1321 tcggtgttca atcgagctac ctgccctggt cgatcaccac cccgtcctta ctcccgaaaa
     1381 cgaaggcttg catgcttttg tcgtttttga acagggccgc catgacagcc ggcggcccgc
     1441 ttcaagcgcg cctcgagggt tcaaattcgc aagtgcggcg cgagagagta gaaaatggga
     1501 gtatacgccc ggacggttaa gccgctagcc gcttcatttc ggcgtggagg tccgatttac
     1561 cttcgaagcc gatgccgggc agttcaggca tcacgatgtg tccgctttcg acgcggacgc
     1621 cgtcaggaaa gccgccatag ggctgaaaca gatcgggata gctctcattg ccgccgaggc
     1681 cgaggccagc cgcgatgttc agcgacatct gatgtccgcc atgcgggatg cagcggctcg
     1741 gcgaccagcc gtgggcatgc agcaccgcca gcgtacgttg atattcgcag agcccatagg
     1801 acagcgcgca gtcgaattgc agccagtccc ggtcggggcg catgccgccg tagcggatga
     1861 gattccttgc gtcctgatgg ctgaacaggt tctcgccggt tgccatcggc gcgggataga
     1921 actcggcgag cgccgcctgc agcgcgtagt cgaggggatc gccggcctcc tcgtaccaga
     1981 acaagggata gtcccgcagc atctttgcgt aggcgatcgc ggtttccaga tcgaagcggc
     2041 cgttggcgtc gaccgcaagc tgcgcgtcct tgccgatctc cttgagcacc gcttcgatac
     2101 gttcgcggtc ctccgcgatc ggcgcgccgc cgatcttcat cttcacgaca ttgtagccgc
     2161 gatcgagata accgcgcatc tcgctgcgca gcatcgacag gtccttgcct ggatagtagt
     2221 agccgccggc ggcatag
//
LOCUS       DATLJT010000424         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2321901,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000424 DATLJT010000000
VERSION     DATLJT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321901"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="VM910_09805"
     CDS             complement(<1..128)
                     /locus_tag="VM910_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62861.1"
                     /translation="MLLSEELPEADGEPKKIVVMGEELLAFRDSRGVVGVIDQYCP"
     gene            complement(350..1246)
                     /locus_tag="VM910_09810"
     CDS             complement(350..1246)
                     /locus_tag="VM910_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI62862.1"
                     /translation="MSSLSGKQGPRYRHTADDGEPYETIAVEKLTPIIGAEISGVDLG
                     RLVSDDTRSNRQMDEIHRALAENLVIFFRNQHITPEQHLAFGRKFGELHIHPAAPHEG
                     DDPALMKIYADKDSPRANGEGWHTDVSCDIEPPMGSILYIKQCPPRGGDTLFANMYAA
                     YEALSDRMKAYLDGLTALHDGESAYRGLYANHGVADKPSYPSAEHPIVRTHPVTGKKA
                     LYVNRGFTRHIVGIPRDESDAILSYLYQHEENPLFQCRFRWTENAIAFWDNRCTQHRA
                     MWDYWPHTRAGTRVTVKGERPV"
     gene            complement(1392..2678)
                     /gene="ispG"
                     /locus_tag="VM910_09815"
                     /gene_synonym="gcpE"
     CDS             complement(1392..2678)
                     /gene="ispG"
                     /locus_tag="VM910_09815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVI62863.1"
                     /translation="MNKPEKSLSIDVAGPGARHKTTEVMVGNVAVGAGAPIVVQSMTN
                     TDTADVASTVDQVTALSRAGSELVRITVDREEAAAAVPHIRDGLRKRGISTPLIGDFH
                     YIGHKLLAEYPACAEALDKYRINPGNVGFKNKRDTQFADIIEIANKNNKPVRIGANWG
                     SLDQELLSKLMDENAASANPRDARAVTREAMVQSALLSAARAEELGMPKNRIILSAKV
                     SAVQDLIAVYQELARRSDYAIHLGLTEAGMGSKGIVASSAALGILLQGGVGDTIRISL
                     TPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRD
                     EMPTWKTKYPGVEQLNVAVMGCIVNGPGESKHANIGISLPGTGEAPAAPVFVDGKKFR
                     TLRGPTIAADFKALVIDYIDQRYGSGNKAPAESAAE"
     gene            complement(2703..>3403)
                     /locus_tag="VM910_09820"
     CDS             complement(2703..>3403)
                     /locus_tag="VM910_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI62864.1"
                     /translation="LLFGIEFVLIYQGLRFTSASRAAVFLYTAPFFVALGSYQVLGER
                     LRASQWAGLALSFLGVALAIGVPQPEVDAKVLLGDLMIVGGGALWAATTLVAKGTNLR
                     FAAPEKALAYQVALSIPILGLAAAISGEVMVRLPGPLALSLVIYQAIWVVGITFTLWF
                     ALVKVYSASKLSAFTFITPLFGVLASYLIMHDALTMVFGAAALLVIAGLFLVNRPNRT
                     VPGATDPLLNVTKT"
BASE COUNT          629 a         1046 c         1114 g          614 t
ORIGIN      
        1 tgcgggcaat actggtcgat gacgccgacc acgccgcgcg aatcgcggaa ggcgagcagt
       61 tcctcgccca tgacgacgat cttcttcggc tcgccgtctg cttccggcaa ttcttcggac
      121 agcagcaccg gcagccagaa gcgggcagca agtcgcccat cggggttccg gcgcccgatt
      181 cggtcaggaa tttgttgtcc tctgcacgca gcacggcaaa cccccgggga aaccccttta
      241 tgctagggaa tcttggcgtg aacgagacaa agcgtcaatg cgcgggcagg ttggcgagac
      301 cgtgaccagc cgacttccgg cccatggctg gcgatagtcc acctaacaat cacacgggcc
      361 gctcgccctt cacggtgacg cgggtgcccg cgcgggtgtg cggccagtag tcccacattg
      421 cgcggtgctg ggtgcagcga ttgtcccaga atgcgatggc attctcggtc cagcggaagc
      481 ggcactggaa cagcggattt tcctcgtgct ggtaaagata ggacagaatc gcgtcgctct
      541 cgtcgcgggg aatgccgacg atgtggcgcg tgaagccgcg attgacatag agcgccttct
      601 tgcccgtcac ggggtgggtt cgcacgatcg gatgctcggc gctcggatag ctcggcttgt
      661 cggcgacgcc atggttcgca taaaggccgc gataggccga ttcaccgtca tgcagcgcgg
      721 tcagtccgtc aagatacgcc ttcatgcgat ccgacagcgc ttcataggcc gcgtacatgt
      781 tggcaaacag cgtgtcgccg ccgcgcggcg ggcactgctt gatatagagg atcgagccca
      841 tcggaggctc gatgtcgcag gacacgtccg tatgccagcc ctcgccgttg gcgcgcggcg
      901 aatccttgtc ggcatagatc ttcatcaacg cgggatcgtc gccctcatga ggcgccgcgg
      961 gatggatgtg caactcgccg aacttcctgc cgaaggcgag atgctgctcc ggcgtgatgt
     1021 gctggttgcg gaagaagatc acgaggtttt cggcgagcgc gcgatggatc tcgtccatct
     1081 ggcggttgga gcgcgtgtca tcggagacga gcctgccgag atcgactcct gaaatctccg
     1141 cgccgatgat cggggtgagc ttttcgaccg caatggtttc gtagggctcg ccgtcgtcgg
     1201 ccgtgtgccg atagcggggt ccctgcttac ctgacagcga gctcatgcaa ttcctccggc
     1261 ttatgtcgcg gccgcatcgt agctccaagc agaagaagcg caacatgctc ggctgtcgtc
     1321 ctggacaagc gagctcctcg cggcgcgtag cgccgtcaag tcacgagcac cgatccggga
     1381 cgacagtcgg actactcagc cgcgctctcg gcgggcgcct tgttgccgct gccgtagcgc
     1441 tgatcgatat agtcgatcac cagggccttg aagtcggcgg cgatggtcgg cccgcgcagg
     1501 gtgcggaatt tcttgccgtc gacgaacacc ggcgcagccg gcgcttcgcc ggtgcccggc
     1561 aaggagatgc cgatattggc gtgcttggat tcgccggggc cgttgacgat acagcccatg
     1621 accgcgacgt tgagctgctc gactcccgga tatttcgtct tccaggtcgg catttcgtcg
     1681 cggatgaaat cctggatcga gcgcgccagc tcctggaacg tggtcgaggt ggtgcggccg
     1741 cagcccgggc aggccgcaac cagcggcacg aaggtgcgga agcccatggt ctgcagcagc
     1801 tcctgcgcga cctgcacttc gagcgtgcgg tcgccgccgg gctcgggcgt cagcgaaatg
     1861 cggatggtgt cgccgacgcc gccctgcagc aggatgccga gcgcggccga tgacgccacg
     1921 atacccttcg agcccatgcc ggcctcggtg aggccgagat ggatcgcgta gtccgagcgc
     1981 cgcgcgagct cctgatagac cgcgatcagg tcctgcacgg cggacacttt tgccgacagg
     2041 atgatgcggt ttttgggcat gccgagctct tcggcgcgcg cggccgagag cagcgccgat
     2101 tgcaccatgg cttcccgcgt cacggcgcgg gcatcgcgcg gatttgccga ggccgcattc
     2161 tcgtccatca gcttcgagag cagctcctgg tcgagcgaac cccaattggc gccgatccgc
     2221 accggcttgt tgttcttgtt ggcgatctcg atgatgtcgg caaattgcgt gtcgcgcttg
     2281 ttcttgaagc cgacattgcc gggattgatg cggtatttgt cgagcgcctc cgcacaggcc
     2341 ggatactcgg ccagaagttt gtgcccgata tagtggaagt cgccgatcag gggcgtggag
     2401 atgccgcgct tgcgcaaccc gtcacggatg tgcggcacgg cggcagcggc ctcctcgcgg
     2461 tcgaccgtga tgcgcaccag ttcggaaccg gcgcgcgaga gcgccgtcac ctggtccacg
     2521 gtgcttgcga catcggcggt atcggtgttg gtcatcgact gcacgacgat cggcgcgccg
     2581 gcgccgacgg cgacgttgcc gaccatgacc tctgtggttt tgtgccgcgc gccggggccg
     2641 gcgacatcaa tggaaagcga tttttcgggc ttgttcatga ggtttttcct gggcctcgaa
     2701 tatcaggttt tggtgacatt cagcaacgga tccgtcgccc cgggaaccgt ccggtttggt
     2761 cggttaacca gaaaaagccc tgcaattaca aggagggctg cggcaccgaa caccatcgta
     2821 agggcgtcat gcatgatcaa atagctagcg agaacgccaa acaaaggggt gatgaaggta
     2881 aatgccgaca atttgctcgc cgaatagacc ttcaccagcg caaaccacag cgtgaacgtg
     2941 atcccgacga cccaaatcgc ctgatagatc accagagaca gcgcaagcgg cccgggaagg
     3001 cggaccatga cctcgccgga aatggcggct gccagtccca aaatcgggat cgacaacgcg
     3061 acctgatagg caagagcctt ttccggcgcg gcaaagcgca aattggttcc cttggcgacc
     3121 agcgtggtcg ccgcccatag cgccccgccg ccgacgatca tgagatcgcc gagcagcacc
     3181 tttgcgtcga cttcgggctg cggcactccg atcgcgagcg cgacaccgag gaagctcaac
     3241 gcgagcccgg cccattgcga ggcgcgcagc cgttcgccca gcacctgata ggagccgagc
     3301 gcgacaaaga acggcgcggt gtagaggaac accgcggcgc gggaggccga ggtgaagcgc
     3361 aggccctgat agatcagaac gaactcgatg ccgaacaaga gtc
//
LOCUS       DATLJT010000425         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1173581,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000425 DATLJT010000000
VERSION     DATLJT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173581"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..457)
                     /locus_tag="VM910_09825"
     CDS             complement(236..457)
                     /locus_tag="VM910_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62865.1"
                     /translation="MNRLVEIRSQEQMCRERAALDSEHRIFWLAQAEEWEQRALDEIA
                     YHFRECNLDYSDPEQRTQRWIASSCLYEI"
     gene            complement(630..1835)
                     /locus_tag="VM910_09830"
     CDS             complement(630..1835)
                     /locus_tag="VM910_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HVI62866.1"
                     /translation="MNTQLIADAMREGTTEDESTIWDRIYAFWLRRLVYTQIWEDPEA
                     DLAALQLPLGSTIVTISSGGCNALSYLTAQPAQVYAVDLNEAHLALLKLKLAGIRAFS
                     NYSEFWQFFGEAASSSNAQLYRDRLRPLLDQQARAYWDKRSVVGRPRHAHFTDGFYRH
                     GMLGRLLGLGHIVAKFARIDLAALLTAELDAPERAQALQRLHRLFHSRLVRLITRTPA
                     LLFGLGLPPQQRARLGRGAPLNEVLHQRLLRLINGHPSDANYFAWQALRRAYRGPGDH
                     CLPPYLQRSQFARMRNDAGLIIPVHASLRQFLDGLPAREVDAVMLLDSQDWMAPEEIR
                     KLWNAIDRTGSDSVRVIFRTAAPESPLESAALSSLKGIWRRDVERSAIGYELDRSGVY
                     GGFHCYVRC"
     gene            2255..>2559
                     /locus_tag="VM910_09835"
     CDS             2255..>2559
                     /locus_tag="VM910_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HVI62867.1"
                     /translation="MSAWAALLAAGLLEVAWALGLKYSDGLTRFWPSMATVAAIVLSF
                     ALMSLALRSLPFGTAYAVWTGIGAVGSIIVGMLLYSEPTDPVRIVCLTLIVAGMVGL"
BASE COUNT          539 a          772 c          776 g          472 t
ORIGIN      
        1 gcagggtcgt tgccgatgta tgtgcgcgac gtcgagccgc attggaccat ggcgaagatc
       61 gacttcatct acgagtgctc ggaatgcggc gcagaggtga ggcagaccat tatcaaaccg
      121 cagtggcagc actgacggac cgagcaatcc cgccatggcc gggagccgca cgtagagcgg
      181 atcgtcccga ttgatctcgc gcgcaaagga gttcatcgcg tcgcgggcgc aatgatcaga
      241 tctcgtaaag gcagcttgat gcgatccagc gctgggtgcg ctgttccggg tcggagtagt
      301 cgagattgca ttctctgaag tgatacgcga tctcgtccag cgcgcgctgt tcccactctt
      361 cggcttgtgc gagccaaaaa atcctatgct cggaatctaa cgcggcgcgc tctcggcaca
      421 tctgctcctg actgcgtatc tccacgagcc tgttcatgac ccccactccc gaggtgaagt
      481 ccattaacgg cggcgaacct agcaggtttg caacagagtg tctcagccgc ctgtgcaaaa
      541 ccggccggca tcacgcagtt gcgaacgcgc gagacatcgc agcgcaacac gaccactgga
      601 ctcgcgatct cgaatagaaa ttccttcgat cagcaccgca catagcagtg gaacccgcca
      661 tagacgcccg atcgatccag ttcgtagcct attgcgctgc gctccacgtc gcgccgccaa
      721 atacctttca aggatgaaag tgccgcactc tcgagcggcg attcaggagc ggcggttcgg
      781 aagatgacac gaacactgtc gctgccggtg cgatcgatgg cattccacaa tttgcggatt
      841 tcctcgggcg ccatccagtc ctgagaatcc agcagcatca cggcatcgac ctcgcgcgcc
      901 ggcagtccgt ccagaaattg tcgcaagctt gcgtgaaccg ggatgatcag gccggcgtcg
      961 ttacgcatgc gcgcaaactg gctgcgctgc agataaggcg gcaggcagtg atcccccgga
     1021 ccgcgataag cgcggcgcaa agcttgccag gcaaaataat tggcgtcact gggatgccca
     1081 ttgatgagac gcaacagccg ttgatggagc acctcgttga gcggcgcgcc gcgtcccagc
     1141 cgtgcgcgct gttgcggcgg aaggccgagg ccgaacagca gggccggcgt gcgcgtgatc
     1201 agccggacaa ggcgcgaatg gaatagccgg tgcagtcgct gcaacgcctg ggcacgctca
     1261 ggtgcatcga gttcggcggt caacaacgcg gcaaggtcga tccgggcaaa tttcgccacg
     1321 atgtgcccga gcccgagcaa acggccaagc atgccatggc gataaaatcc gtccgtgaaa
     1381 tgggcatgcc tcggacggcc gacgacgctc cgcttgtccc aataggcacg agcctgctga
     1441 tcgagcaacg gccgcagccg gtcgcgatag agttgcgcat tggaggacga agccgcctcg
     1501 ccgaagaact gccagaattc ggaataattc gaaaaagcgc ggataccggc gagcttgagc
     1561 ttgaggagcg cgagatgcgc ctcgttgaga tccactgcgt agacctgggc cggctgcgca
     1621 gtcaggtacg acagcgcgtt gcagccgccg gatgaaatgg tgacgatggt cgaccccaaa
     1681 ggcaattgca gcgcggcaag gtcagcctcc gggtcttccc agatctgggt gtagaccagg
     1741 cgacgcagcc agaaggcata aatcctgtcc caaatcgtcg attcatcttc cgttgtgccc
     1801 tcgcgcatcg catcagcgat caactgcgta ttcatcagag cctcgaatca acaatccgac
     1861 gaggctaacg ctcgtgcact tcagttttgc gacaaccgcc aggcacaggg gatgaggatg
     1921 tgaacccttg tcacatgctt gtcgcgcgaa caacgcaagg agggagcgaa cacttcacca
     1981 agagatttcg ccaagagatg atctgaatgt cggcgcatcc tttcgacatc ggccctgaaa
     2041 cccgagatcg gatcgatacc ttgcaggccg cacacttccg cggcatcgaa gtgccgcgaa
     2101 atacgccggg ttccatcgga actcgcgcgg tcactgacgg caaagccagc cccttccgct
     2161 tcacgcgagg cggcgctacg cacactgatc cacagatttt attcgggttc cgccgcgtcg
     2221 atcgcgacgt gcctgcatat gcgtggagag cgccatgagc gcctgggccg cacttctcgc
     2281 tgcaggactg ctcgaggtcg cctgggcgct tgggctcaaa tattccgacg ggctgaccag
     2341 attctggcca agcatggcaa ctgtggccgc gatcgtgctc agctttgctt tgatgtcatt
     2401 ggcgctgcga tcactgccct tcggcacggc gtatgcggtg tggaccggga taggcgccgt
     2461 cggcagcatt atcgtcggga tgctgctcta cagcgagccg actgatccgg tccgcatcgt
     2521 ttgcctcacg ttgatcgtcg ccgggatggt tgggcttaa
//
LOCUS       DATLJT010000426         2518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3011209,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000426 DATLJT010000000
VERSION     DATLJT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3011209"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..361)
                     /locus_tag="VM910_09840"
     CDS             complement(176..361)
                     /locus_tag="VM910_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62868.1"
                     /translation="MLKILTATAASLAMLGAAAAADLPRKEPPPPAPPVGKAPIGKYP
                     VGKAPIGKYPQPVVTKG"
     gene            561..1259
                     /locus_tag="VM910_09845"
     CDS             561..1259
                     /locus_tag="VM910_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62869.1"
                     /translation="MRDFFRSVRFGAAWLMGLALLSPLAAQSPADQQAAEGTVNIYNL
                     DREAHVGGVEIHGLISKAVASQAMQLIRTIRPDVDDLKVLLSSPGGDVLAAMELGEEV
                     RKQSAWTTVDENGECFGACVLVLAAGVRRIPTPENVGLQRMNFDQKEFVANLSPDKAK
                     QKYTGVAKRVETYLARMGMPKKLFQEMSAQQSSAKVRLLDAAKLKSLGLDGIDPAYEQ
                     WLRENNIQQPPPSN"
     gene            1491..>2518
                     /locus_tag="VM910_09850"
     CDS             1491..>2518
                     /locus_tag="VM910_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI62870.1"
                     /translation="MRPIVRAFVLAAVALAWSKPASAQTPADIVLLNGKIVTVDDRFT
                     VAEALAIKGERILAVGSNAEIETHKGPLTRVVDLNRRTVIPGLIDNHAHYMRAAEYWH
                     REVRLDGITSHREALDLIKHKAAESKPGEWVVVLGGWAEEQFVDEPRGFSRAELDGIA
                     PNNPVALQLFYFRVYANTAALKAMGIGPATPDPTGIKIEKDDKGELTGALNGGPSIGL
                     LRDKLGAVARDKAVENARLLMHDLNKMGITAFQDQGGTGMKASHIEAFHMAHDAGQMT
                     VRSFYNYYEEPRSVADVENLIARMAQIRPFQGDDWFDLTGYGETLYFPLHDALLAKAA
                     NPSAEALQL"
BASE COUNT          528 a          764 c          775 g          451 t
ORIGIN      
        1 cgcaatccac gcctgctggt agggcgggtt cgccagttgc tggcggtcgc gcgccgtgaa
       61 attggcatcg aacactggac cggactgcat gcagcccgca agcagcagtc cggtcaaaac
      121 aacgacgatg ccccgcaccg acggcatggc ctacgtccgc gcgtatgtgc ggcggtcagc
      181 ctttggtcac gacgggctgt ggatacttgc cgatcggagc cttgccgacc ggatacttgc
      241 caatcggagc cttgccgacc ggaggcgccg gaggaggcgg ctccttgcgc ggcagatcgg
      301 cggcggcggc agcgcccagc atggccaagg acgcagcggt tgcggttaga atcttcagca
      361 tctgaccccc catgtttgat ggccgaattt cccaccgctg gaagtgcccg acaatggaaa
      421 aggcttgagc cgagcgcttt ttctggcatc tgttgcacaa acgccacctc cggcgaacgc
      481 gacgtccttc accatacggt gcgctccccc agatttcgcc ccaattttca acgtagtagg
      541 aatgaatttc gggattgggc atgcgggact tcttcagaag cgtgaggttc ggcgcggcgt
      601 ggctgatggg cttggccctt ctctcgccgc tggccgcaca atcgcccgcc gaccaacaag
      661 ccgccgaggg caccgtcaat atttacaact tggaccgtga ggcacacgtc ggcggggtcg
      721 agattcatgg tctgatcagc aaagccgtgg ccagccaagc catgcagctg atccggacca
      781 tccgtccaga cgttgatgac ctgaaggtgc tcctcagctc gccgggagga gatgtccttg
      841 cggcgatgga gctgggcgag gaagtcagga aacaatcggc ttggacaacc gtggacgaga
      901 acggggagtg cttcggcgca tgtgtgctcg tcctggcggc aggcgtgcgg cgaatcccta
      961 cgcccgaaaa cgtggggctt cagcgcatga acttcgacca aaaagagttc gtcgcgaacc
     1021 tctcccccga taaagccaaa caaaaataca ctggggtggc gaaacgggtc gagacttact
     1081 tggcccgcat gggaatgcca aaaaagctct tccaagaaat gagcgcccag caatcgtcgg
     1141 ccaaagtgcg tctgctcgat gctgctaaac tcaaatcgct gggactggat ggcatcgatc
     1201 cggcttatga acagtggctg cgcgagaaca atattcagca accaccgcca tcaaactgag
     1261 aacgactacg tctgtagggt gatttcgcaa acaagccctt gcggacgcca atcacgcgct
     1321 ttgcctttta atcgtccaat cattcgtttg acgtcccgac cgccaaaacc ctgacgcgcg
     1381 ggcggctgta ctgcctaaat catccatctc ggtacggctg attttgccct cgcattgacc
     1441 cgcgtttgcg gcattattga atggcaagca gctgctttga ggaaaccatc atgcgcccca
     1501 tcgtacgtgc ctttgtcctt gctgccgtcg ccctcgcctg gagcaagccc gcgtcggcgc
     1561 aaaccccggc ggacatcgtg ctactcaacg gcaagatcgt cacggtggac gaccggttca
     1621 cggttgccga ggcgctggcg atcaagggcg aacgcatcct ggcggtcggc agcaacgccg
     1681 agatcgagac gcacaagggg ccgctcacgc gcgtggtgga tctcaatcgg cgcaccgtca
     1741 tccctgggct gatcgataac cacgcgcatt acatgcgcgc cgccgaatac tggcatcgcg
     1801 aagtgcggct cgacggcatc acgtcgcacc gagaagcgct cgacctgatc aagcacaagg
     1861 cggccgaatc caagccagga gaatgggtgg tcgtgctggg gggctgggcg gaagagcagt
     1921 tcgtggacga accgcgcggc ttcagcaggg cagagcttga cggcatcgca ccgaacaatc
     1981 cggtggcgct gcagctgttc tacttccgcg tctatgccaa caccgcggcg ctcaaggcga
     2041 tggggatcgg accggccaca cccgatccca ccggaatcaa gatcgagaag gacgacaagg
     2101 gcgagctgac tggcgcgctc aacggcggcc cttcgatcgg tctcttgcgc gacaagctcg
     2161 gggcggtggc acgtgacaag gcggtcgaga acgcgcggct tctgatgcac gatctcaaca
     2221 agatgggcat caccgcgttc caggaccaag ggggaaccgg aatgaaggcg agtcacatcg
     2281 aggcgtttca catggcccac gacgccgggc agatgacggt gcgctcgttc tacaattatt
     2341 atgaggagcc ccgttcggtc gcggacgtcg agaacctcat cgccaggatg gcgcagatca
     2401 ggccgttcca gggcgatgac tggttcgatc tgaccggtta cggcgagact ttgtatttcc
     2461 cattgcacga cgcgctgctc gcgaaggccg caaatccgtc cgccgaagcg ctgcagct
//
LOCUS       DATLJT010000427         1951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3011213,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000427 DATLJT010000000
VERSION     DATLJT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3011213"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VM910_09855"
     CDS             complement(<1..315)
                     /locus_tag="VM910_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62871.1"
                     /translation="MTDDRSEATTSGQLRPPGLGLFLAEARGIFELNASLLLLPFLMR
                     APRGDGHPVLTLPGFLASDLSMAPMRHYLSELGYDTHAWRMGRNTGGVSRMRSALLDR
                     LRA"
     gene            complement(315..572)
                     /locus_tag="VM910_09860"
     CDS             complement(315..572)
                     /locus_tag="VM910_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HVI62872.1"
                     /translation="MKVNVEIDCTPLEARQFFGLPDVVPMQTAVMDKLQQQMTSNIEK
                     ISPESLIQSWFSFDPKLAERFQEMFIAMAGLGGALTGDKKK"
     gene            1298..>1951
                     /locus_tag="VM910_09865"
     CDS             1298..>1951
                     /locus_tag="VM910_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62873.1"
                     /translation="MSVRLSLLAATIASIVAMVISPASSQTLRYANQGELKSLDPYTL
                     KETTTIAHHAHVYEGLVTRDKDLKIIPALAESWETLTPTQWRFHLRKNVKFHNGDPFT
                     ADDVLFSAQRVRSPGSNFLSNVPADAKFVKVDDHTVDVLLDSPNPILTSQWDGWFIMD
                     KKWCEENNSVAPTPASATTPSYASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEH
                     "
BASE COUNT          425 a          638 c          555 g          333 t
ORIGIN      
        1 cgcacgcagc cggtcgagca gcgcgctgcg cattcgcgac acgccgccgg tattgcggcc
       61 catgcgccat gcgtgggtgt cgtagccgag ttcgctgagg taatggcgca tcggcgccat
      121 cgagagatcg ctggcgagaa aacccggcag cgtcagaacc ggatgaccgt cgccgcgcgg
      181 cgcgcgcatc agaaacggca aaagcaggag actggcattg agttcgaaaa tgcccctcgc
      241 ttcagccagg aacaggccca atcccggcgg acggagttga cccgaagtag tcgcttcgct
      301 gcggtcgtcc gtcattactt cttcttgtca ccggtgagcg cgccaccgag ccccgccatc
      361 gcaatgaaca tttcctggaa ccgctcggcg agcttcggat cgaaactgaa ccagctctgg
      421 atcaacgact ccggcgagat cttttcgatg ttcgacgtca tctgctgttg cagtttgtcc
      481 atcaccgccg tctgcatcgg caccacatcg ggaagcccga aaaactgccg ggcttcgagg
      541 ggcgtgcagt ctatctcaac attgactttc atttccgctc ctgaccgcgc ctgtgacagg
      601 cgccgctagt ttggatccga tatttcgctt tgcgagcccg acgaggaagg gatcgcgaga
      661 tggcaaacca gtatcgccgc gacaagcgca cccgcgcaag ccatggatgc aacgcacaac
      721 catcttcgtt gcgcaatccg caagaatggt caaccgaagc gctcggccgc aaacgggctc
      781 ggatcggcaa aacgcgcctc gccgcgcacg atttccgcca acaggcgccc ggaggccggg
      841 ccgagcgcga acccatggtg ccggtggcac ggttcgtcgc gtatcgaacg atctcccggg
      901 acctcgcgta gaaacacgtt gggaaacacc gacgcgcatt cgatgatccc cgtatggccg
      961 aagctcgttt cttagccggc gcgctcatgc cggtcgatca gaatgacgtc gcgaccgcgc
     1021 cgctgcagat gcagggacgg cgctgacgcc gactattccc gcgccaagaa cgagaacgtc
     1081 ggtcttcaac tctgccattc accactccga aagtcgaata tcgatcgcgc acaggcgcag
     1141 cccgaacgaa gccagcgacc gcagcagccc tgcgcaaacc gttcgctttt cgggcggaaa
     1201 tcaagcttgc gcagcggcca aactcgatcc aacatggatc gggaagaatc cgcgaaaatt
     1261 gcaatcatgc agcgccacca gcagagagcg taaagaaatg tcggtacgtc tatccttgct
     1321 agcagcgacc atcgcctcga tcgtcgccat ggtgatatcg ccggcatcga gccagacctt
     1381 gcgttacgcc aaccagggcg agctcaaatc gctcgatccc tatacgctca aggagaccac
     1441 gacgatcgcc caccacgcgc atgtctatga aggcctggtg acccgcgaca aggatctgaa
     1501 gatcattccg gcgctggcgg aaagctggga gaccctgacg ccaactcaat ggcgcttcca
     1561 tctgcgcaag aacgtcaagt tccacaacgg cgatcccttc accgccgacg atgtcctgtt
     1621 ctcggcccag cgcgtgcgca gccccggctc aaacttcctg tccaacgttc cggccgacgc
     1681 gaaatttgtg aaggtcgacg accatacggt ggacgtgctt ctggactcgc ccaatcccat
     1741 cctgacctcg cagtgggacg gctggttcat catggacaag aaatggtgcg aggaaaacaa
     1801 ttccgtcgcg ccgacgccgg cttccgcgac cacgccgagc tacgcctcgc tgcacgaaaa
     1861 cggcaccggg cccttcatca tcgagagcca tcagcccggc gtgaagaccg tcttcaaggt
     1921 gaacccgaac tggtggggca agcccgagca t
//
LOCUS       DATLJT010000428         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1402957,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000428 DATLJT010000000
VERSION     DATLJT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402957"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VM910_09870"
     CDS             complement(<1..923)
                     /locus_tag="VM910_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62874.1"
                     /translation="MLAFILRRAIQAIGVMIVVGVIAFSMFRFAGDPVNQMVSIDTSA
                     AERNAIRHSLGLDDPVLVQFARYFANAARFEFGVSYQFRQPVAHLLKERLPATLELAT
                     CATVLAMVFGILMGVYSALRRDSLLAKAFQAISLIGISLPTFLIGILLIYLFAVVLGW
                     LPSFGRGEVVRFGWWTTGLLTVSGLKALIMPSITLGLFQMTLIMRLVRAEMLEVLRTD
                     YIRFARARGLTTRAIHFGHALRNTLVPVITVAGLQFGSVIAFAIITETVFQWPGMGLL
                     FVQAVQNVDIPIMAAYLLLVSLIFVTINLVV"
     gene            complement(1028..>2116)
                     /locus_tag="VM910_09875"
     CDS             complement(1028..>2116)
                     /locus_tag="VM910_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62875.1"
                     /translation="PTPASATTPSYASLHENGTGPFIIESHQPGVKTVFKVNPNWWGK
                     PEHNLKEIVFTPIGSAATRVAALLSGEVDVIEPVPIQDIQRVNSSGIATVMTGPELRT
                     IFLGMDQARDELLFSNIKGKNPFKDIRVREAFYKAIDVELIKTRVMRGLSTPSALMIA
                     PQLFSLSKDFVRPKFDPDGAKKLLAEAGYPSGFEVTMDCPNDRYVNDAQICEAVVGML
                     ARIGVKVNLLAQPKAQYFAKVLKPGGFQTSFYLLGWTPASSDSHNVLHDIMGCRDDKN
                     DATRGEANLGGYCSKELDALTDKILVEADNTKRDQMIKQAFEIGFKDYAYIPLHQQSL
                     AWGVSKKVKLTQRADNAVLLYWATKQEE"
BASE COUNT          432 a          622 c          679 g          383 t
ORIGIN      
        1 tcgaccacga gattgatggt gacgaagatc agggagacca gcaacaggta tgccgccatg
       61 attggaatgt cgacgttctg cacggcctga acgaacaaca gtcccattcc gggccactgg
      121 aacacggttt cggtgatgat cgcgaatgca attaccgagc caaactgcag gccggcgacg
      181 gtgatgacgg gaaccagggt gttcctgagc gcgtgtccga agtgaatggc acgcgtggtc
      241 agaccgcgcg cgcgggcgaa gcggatgtaa tcggtgcgca gcacctccag catctcggcg
      301 cgcaccagcc gcatgatcag cgtcatctgg aacaagccaa gcgtgatcga cggcatgatc
      361 aaggctttga gcccggagac ggtcagcagg ccggtggtcc accagccgaa ccgcaccact
      421 tcgccgcggc cgaacgacgg cagccagccc aggaccaccg cgaacaggta aatcaggaga
      481 atgccgatca aaaatgtcgg cagcgaaatt ccgatcagcg agatcgcctg gaacgccttg
      541 gccagcagcg agtcgcgccg cagcgcagag tagacgccca tcaggatgcc gaacaccatc
      601 gcaagcacgg tggcgcaggt ggcaagctcg agcgtcgcag gcagtcgctc tttcaacagg
      661 tgggcgaccg gctggcggaa ctgataggag acgccgaatt caaacctggc cgcattggcg
      721 aagtagcggg caaactgcac cagcacggga tcgtcgagac cgagcgagtg gcggatcgca
      781 ttgcgctcgg cggccgatgt atcgatcgag accatctggt tcaccgggtc tccggcgaag
      841 cggaacatcg aaaaagcgat cacaccgacg acgatcatga cgccgatggc ctggattgct
      901 cgacgaagaa tgaaggcgag catttgaccc cttcgatctt ccaaacgcag ttcgcgcgcc
      961 gttgcgctgc cttaagcgaa aggtcccgga agcgcgacgc tgccgggacc tttgccgatg
     1021 agactgacta ctcttcctgt ttggtcgccc agtacagcag taccgcgttg tcggcgcgct
     1081 gggtgagctt cacctttttc gacacgcccc aggccagcga ctgctggtgc agcgggatat
     1141 aggcgtaatc cttgaagccg atctcgaagg cctgcttgat catctgatcg cgcttggtgt
     1201 tgtcggcctc gaccaggatc ttgtcggtca gcgcgtcgag ttccttgctg cagtagccgc
     1261 cgaggttggc ctcaccacgc gtggcatcgt tcttgtcgtc gcggcagccc atgatgtcgt
     1321 gcagcacgtt gtgcgaatcg gatgaagccg gcgtccagcc caggagatag aacgacgtct
     1381 ggaagccgcc gggcttcagc accttggcga aatactgcgc cttgggttgg gcaagcaggt
     1441 tcactttgac gccgatgcgg gcgagcatcc cgaccacggc ctcgcagatc tgggcgtcgt
     1501 tgacatagcg gtcattcggg caatccatcg tgacctcgaa gccgctggga taaccggcct
     1561 cggccaagag cttcttggca ccatcaggat cgaatttcgg ccgcacgaaa tccttcgaga
     1621 gcgagaacaa ttgcggcgcg atcatcaacg ccgacggcgt cgacaggccg cgcatcacgc
     1681 gtgtcttgat cagttcgacg tcgatggcct tgtagaaggc ttcgcgcacg cggatgtcct
     1741 tgaagggatt cttgcccttg atgttcgaga agaggagttc gtcgcgggcc tggtccatac
     1801 cgaggaaaat ggtgcgcagc tccggcccgg tcatgacggt ggcgatcccg ctggaattga
     1861 cccgctggat atcctggatc ggaaccggct cgatgacgtc gacctcgccg gagagcaggg
     1921 cggcgacgcg cgtcgcggcg gaccctatcg gcgtgaagac gatttccttc agattatgct
     1981 cgggcttgcc ccaccagttc gggttcacct tgaagacggt cttcacgccg ggctgatggc
     2041 tctcgatgat gaagggcccg gtgccgtttt cgtgcagcga ggcgtagctc ggcgtggtcg
     2101 cggaagccgg cgtcgg
//
LOCUS       DATLJT010000429         5969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_24716,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000429 DATLJT010000000
VERSION     DATLJT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24716"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VM910_09880"
     CDS             <1..835
                     /locus_tag="VM910_09880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498502.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVI62876.1"
                     /translation="DCQAIVKGMFNNHRFRDAVALSGVNSINWARIVAQVVYYFTSAV
                     ALGAPARQVDFTVPTGNFGDIFAGYVAKRMGLPVRSLRIAANINDILPRTLKTGHYEV
                     REVHATTSPSMDIQVSSNFERLLFEASGRDASTVRRLMEQLKQSGRFVLPDAMLAAIR
                     EEFDAGRADETETSAAIRAAWREAGDLVDPHTAVALAVADRDTSDSRIPNIVLSTAHA
                     AKFPDAVEAACGVRPQLPPWLDGLMSKPEQIRIAKNDQATVEKFVLSVSRAAAKQGVA
                     G"
     gene            832..2121
                     /locus_tag="VM910_09885"
     CDS             832..2121
                     /locus_tag="VM910_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVI62877.1"
                     /translation="MSVEVTKLETGLTVVTDTMPHLETAALGVWAGVGGRDEKPNEHG
                     ISHLLEHMAFKGTANRTSRQIVEEIEAVGGDLNAGTSTETTAYYARVMKADVPLALDV
                     LSDILTNPSFVPEELEREKNVIVQEIGAAQDTPDDVVFEHLNELCYPDQPMGRSLLGT
                     AKTLKHFDRDMLRGYLATHYRGPDMVVAAAGAVDHQRVVADVAARFRSFNGPAAPKPL
                     PAMFGKGGSRVVHRELEQAHLTLALEGVPQTDLSLFSLQVFTNTVGGGMSSRLFQEVR
                     EKRGLCYSIYAFHAPYTDTGFFGLYTGTDPADAPEMVEVVVDVINEAVETLTEAEVAR
                     AKAQMKAGLLMALESCSSRAEQLARHVLAYGRPQTTDELLARIDAVSVESTRNAARAL
                     LTRSRPAVVALGSGRGLDTAVSFAEGLTRPTAKARLH"
     gene            2245..2832
                     /locus_tag="VM910_09890"
     CDS             2245..2832
                     /locus_tag="VM910_09890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVI62878.1"
                     /translation="MALFRLPSAGPPALAPRGNGLLLRAPQMSDFMQWTQLREYSREY
                     LTPWEPIWPSDDLTRSGFRRRLRRYAEDIAADRSYPFIIFRESDGAMVGGITLANVRR
                     GIVQAGTIGYWVGQPYAHKGYMTSALRVLLPTLFGELNLHRVEAACIPSNAPSIRVLE
                     KCGFSREGLARRYLCINGVWQDHLLFGLLSEDFRG"
     gene            2922..3563
                     /locus_tag="VM910_09895"
     CDS             2922..3563
                     /locus_tag="VM910_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62879.1"
                     /translation="MVEKHLIRTVGVAAIMLLIGSGALVSPSSAQSLTDRFKSLFGGK
                     SDEPVQQAPVVSAGPEPEVNCPPVTIRAGASTYAVAAPGKQAVGNDVRFQASITKTAR
                     DCTKAGGEITARIGIQGRVIAGPAGAPTSIEVPLRVAVVQGGVTEKVITTKAYKTTVA
                     MGEGENVPFTLVADDVVYPIPPGAVGDSYIFYIGFDPQLVSPESKPKAAKKKK"
     gene            complement(3646..4131)
                     /locus_tag="VM910_09900"
     CDS             complement(3646..4131)
                     /locus_tag="VM910_09900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796342.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HVI62880.1"
                     /translation="MFEEPETWVAIAFVILMGVFAYFGVHRTVLKALDNRSARIKAEL
                     DDARRLKDEAATVLAESKNRRASAEREADEIITSAKAEAERIAGEAKTKMEDFVARRT
                     KTAESKIALAEAQALADVRAAAADAAVQAASTILSKSVKGEVADDLLSKGIAEVRQKL
                     N"
     gene            complement(4136..4696)
                     /locus_tag="VM910_09905"
     CDS             complement(4136..4696)
                     /locus_tag="VM910_09905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HVI62881.1"
                     /translation="MAESHGGTENTTAHTEADGGEHNGGFPPFDPSTFPSQLVSLAIC
                     FVALYVIVSRIALPRVGGVIDARHNKIEGDLSDAQKLKDESEAALKAYESELAAARSK
                     AQTMAAQNREKLNAAADAERKTLEQKLATKLADAEKSIAATRTAAMKNVRGIAADAAS
                     AIVQRLTGTAPDGKTVEAAVDASLKG"
     gene            complement(4757..4987)
                     /locus_tag="VM910_09910"
     CDS             complement(4757..4987)
                     /locus_tag="VM910_09910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HVI62882.1"
                     /translation="MDPAAAKLIGAGIACIGMGGAGVGVGVIFGNYLAAAVRNPSAAQ
                     GQFGNLIFGFAVTEALGIFSLLVALLLLFVKL"
     gene            complement(5042..5797)
                     /locus_tag="VM910_09915"
     CDS             complement(5042..5797)
                     /locus_tag="VM910_09915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVI62883.1"
                     /translation="MTLDPIHQFNIDNLFTIGHIGGQPIYFTNSSAYMLVAVAVICLL
                     MIGGVAGRQLVPGRFQSMAEVSYEFVASMIRSNAGAEGMRFFPLVFSLFMFILVSNLV
                     GIIPYTFTVASHIIITATFALLVFFTVLMYGFYKNGMKFFKIFVPSGVPIYILPLVVT
                     IEIISFFSRPLSHSVRLFANMLAGHITLKVFAGFVAMLGTSLGAIGWIGGMLPLALTV
                     ALTALELLVAFLQAYVFAILTCIYLNDALHPGH"
     gene            complement(5863..>5969)
                     /locus_tag="VM910_09920"
     CDS             complement(5863..>5969)
                     /locus_tag="VM910_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVI62884.1"
                     /translation="LSTSPFGFIVFFLLGFVAGVVNVVRSAGVVPRDR"
BASE COUNT         1095 a         1839 c         1960 g         1075 t
ORIGIN      
        1 cgattgccag gcgatcgtga agggcatgtt caacaatcac cgcttccgcg atgccgtcgc
       61 gttgtcgggc gtcaactcga tcaactgggc gcggatcgtc gcgcaagtcg tctattattt
      121 cacgtccgca gttgcgctcg gggcgccggc gcggcaggtg gacttcaccg tgccgaccgg
      181 caatttcggc gacatctttg ccggctacgt cgccaagcgc atgggcctgc ccgtccgctc
      241 attgcgcatc gccgccaata tcaacgatat cctgccgcgc acgctgaaga ccggtcacta
      301 tgaggtccgc gaggtgcacg ccaccacgtc gccgtcgatg gacattcagg tctcctccaa
      361 tttcgagcgc ttgttgttcg aggcgagcgg ccgcgatgca tctaccgtgc ggcggctgat
      421 ggagcagttg aagcaatccg gacgcttcgt gctgccggac gcgatgctgg cggcaatccg
      481 cgaggagttc gacgccggcc gcgcggatga gaccgaaacc tcggccgcga tccgcgccgc
      541 ctggcgtgaa gctggcgatc tcgtcgatcc ccacaccgca gtggcgctgg cggtggctga
      601 ccgcgacacc tcggattcga ggatccccaa catcgtgctc tcgaccgcgc atgcggcaaa
      661 atttcccgat gcggtggaag cggcttgcgg ggtccggccg caattgccgc cctggctcga
      721 cgggctgatg tcaaagcctg agcagatcag gatcgccaaa aacgaccagg ccacggtcga
      781 aaagttcgta ttgtcggtca gccgcgccgc ggcaaagcaa ggagtagccg gatgagcgtt
      841 gaagttacca agctcgagac cggcctcacc gtggtcaccg ataccatgcc gcatctggag
      901 actgcggcgc tcggcgtgtg ggccggcgtc ggcggccgcg acgaaaagcc gaacgagcac
      961 ggtatttcgc atctcctgga acatatggcg ttcaagggca ctgccaaccg aacctcgcgc
     1021 cagatcgtgg aagagatcga ggcggtcggc ggtgatctca acgccggcac ctcgaccgag
     1081 accacggcct attacgcgcg ggtgatgaag gccgacgtgc cgttggcgct cgacgtgctc
     1141 tccgacattc tcaccaatcc atccttcgtg ccggaggaac tggagcggga aaagaacgtg
     1201 atcgtgcagg agatcggcgc ggcacaggat acgcccgacg acgtcgtttt cgagcatctc
     1261 aacgagctct gctatcccga ccagccgatg gggcgttcgc tgctaggaac ggcgaagaca
     1321 ttgaagcact tcgaccgtga tatgctgcgc ggttatctcg cgacgcatta tcgtgggccc
     1381 gacatggtgg tggccgcggc aggcgccgtc gaccaccagc gggtggtcgc cgacgtcgct
     1441 gcacgattca ggagcttcaa tggcccggct gcgccgaagc cgctgccggc gatgttcggc
     1501 aagggcggct cgcgcgtagt gcatcgcgag ctcgagcagg cgcatctgac gctggcgctg
     1561 gaaggcgtgc cgcagaccga tctctcgctg ttctcgctgc aggtcttcac caatactgtc
     1621 ggcggtggaa tgtcgtcgcg gctgttccag gaggtgcgcg agaagcgcgg gctctgctat
     1681 tccatctatg cgttccacgc gccctatacc gacaccggct ttttcggcct ctataccggt
     1741 accgatcccg cggacgcgcc ggaaatggtg gaggtggtgg tcgacgtcat caacgaggcc
     1801 gtggagaccc tgaccgaagc cgaggtcgcg cgggcgaaag cgcagatgaa ggccgggcta
     1861 ttgatggcgc tggaaagctg ctcgtcgcgc gccgagcagc tcgcgcggca cgtgctggcc
     1921 tatggcaggc cgcagacgac cgatgagctc ctggcccgga tcgatgccgt cagcgtcgaa
     1981 tcgacgcgga atgctgcccg cgcgctgctt acgcgcagcc ggcccgccgt ggtcgcgctc
     2041 ggcagcggca ggggtctgga cacggcggtc agttttgcgg aaggattgac gcggccgaca
     2101 gcgaaggcgc ggctgcacta gtgtggcggt ttcgaagtcg gcatcattcc tggccatcgc
     2161 attcgtttcg gcggtggagg gatttcaaaa tcggcggcac gagggcgata aagtctgcta
     2221 gagttgcgcc tcggggagca tcatatggct ctgtttcgtt tgccatcggc tggaccgccc
     2281 gcgctggcgc cgcgcggcaa cggattgttg ttgcgtgcgc cgcagatgtc ggacttcatg
     2341 caatggacgc aactgcgcga atacagccgc gaatatctga cgccgtggga gccgatctgg
     2401 ccctccgacg acctgacccg gtccggtttt cgccgccgtt tgcgccgtta cgctgaggac
     2461 atcgccgctg accgctccta tcccttcatc atctttcgcg aatcggatgg tgcgatggtc
     2521 ggcggcatca cgctcgccaa tgtacgccgc ggcatcgtgc aggccggcac catcggctat
     2581 tgggttgggc agccctatgc gcacaagggc tacatgacgt cggcgctgcg cgtgctgctg
     2641 ccgaccctgt tcggcgagct caatctgcac cgggtcgagg ccgcctgtat tccctccaat
     2701 gcgccgtcga tccgggtgct ggagaaatgc ggcttttccc gcgaagggct cgcacggcgg
     2761 tacctctgca tcaacggcgt ctggcaggac cacctgctgt tcggcctgtt gagcgaggat
     2821 tttcgcgggt aatgcccgac tttcgcatgc cttctgtttg ccgtcatcgc tgctataacg
     2881 ccggaccggg ggtgtcgcgg gacggcaggc agggtcttcc catggttgaa aaacatctca
     2941 tcagaaccgt cggcgtcgcg gcgataatgc ttttgatcgg cagcggcgct ttggtgtcgc
     3001 cgtcttcggc gcagtcgctg accgatcgct tcaaaagcct gttcggcggc aagtcggacg
     3061 aaccggtgca acaggcgccg gtggtctccg cgggccccga gccggaggtc aattgcccac
     3121 cggtgacgat ccgcgccggc gcctccacct atgcggtggc cgcgcccggc aagcaggcgg
     3181 tcggcaacga cgttcgattc caggcaagta tcaccaaaac ggcgcgcgac tgcaccaagg
     3241 cgggcggcga gatcaccgcg cgcatcggta tccagggtcg cgtcatcgcg gggcccgcgg
     3301 gcgcgccgac ctcgatcgag gtgccgctgc gcgttgcggt ggtgcagggc ggcgtcaccg
     3361 agaaggtgat cacgaccaaa gcctacaaga cgacggtggc gatgggcgag ggcgagaacg
     3421 tgccgttcac gctggttgcc gacgacgtgg tctatccgat cccgccgggc gccgtcggcg
     3481 acagctacat tttctacatc ggctttgacc cgcagttggt ctcccccgag tcgaagccga
     3541 aggcggcgaa gaagaagaaa tgacgtcgcc gagttgaagg cccaataaaa aagccggcgc
     3601 cgatattccg cgccggcttt cgttctctgg gaacgtgaaa ctcgctcagt tcagcttctg
     3661 ccgcacctcg gcgatgccct tggagagcag atcgtcggca acctcgcctt tcaccgattt
     3721 cgaaaggatg gtggaggcag cctgcacggc ggcatctgcc gcggcggcgc ggacatcggc
     3781 cagcgcctgc gcctcggcaa gcgcgatctt gctctcggcc gtcttggtgc gccgcgcgac
     3841 gaaatcctcc atcttggtct tggcttcccc cgcgatgcgc tcggcctcgg ccttggccga
     3901 ggtgatgatc tcgtcggcct cgcgctcggc gctggcgcgg cggttcttgg actcggccag
     3961 gaccgtggct gcctcgtcct tgaggcggcg ggcgtcgtcg agctctgcct tgatccgcgc
     4021 gctacgattg tcgagcgcct tcaacacggt gcggtgaacg ccgaaatagg cgaatacgcc
     4081 catcaggatg acgaacgcga tcgcaaccca ggtttccggc tcttcgaaca tcgaactatc
     4141 ccttcaagga ggcatcaacc gcggcttcga cggtcttgcc gtctggagcg gtgcccgtga
     4201 gccgctgcac gatggcagat gccgcatcgg ccgcgatgcc gcgcacgttc ttcatcgcgg
     4261 ccgtgcgggt cgcagcgatg gacttctcgg cgtcggcgag cttggtcgcg agcttctgtt
     4321 cgagcgtctt gcgctccgcg tcagcagccg cattcagctt ctcgcggttc tgggccgcca
     4381 tcgtctgggc cttcgagcgc gccgcggcaa gctcgctttc ataggccttc agcgccgcct
     4441 ccgactcgtc tttcagtttt tgcgcgtcgg acagatcgcc ctcgatcttg ttgtgccgcg
     4501 catcgatcac gccgccaacg cgcggcagcg cgatgcgtga gacgatgacg taaagcgcga
     4561 cgaaacaaat cgcgagcgag acgagctgcg agggaaaggt ggaggggtcg aacggaggaa
     4621 aacctccgtt gtgctcgcca ccatcggcct cggtgtgggc cgtcgtgttc tcggtgccgc
     4681 catgactctc agccatggag ttctcctgtt ggtctcacgc gcaccgcaaa aacggcggcg
     4741 cgaaagggcg caagcttcag agcttgacga acagcaggag cagcgcaacc agcagcgaga
     4801 agatgccgag cgcttcggtc acggcgaagc cgaagatcag gttgccgaac tggccctgcg
     4861 ctgcggacgg attgcgcacg gcggctgcca gatagttgcc gaagatgacg cccacgccca
     4921 cgcctgcgcc gcccatgccg atgcacgcga tgcccgcgcc gataagtttt gctgctgccg
     4981 gatccatttc ttactccttg ggaaaagatg agtggtgtgg gtggaagttc cccgggaccg
     5041 cttagtggcc cggatgaagg gcgtcgttga gatagatgca ggtcaggatc gcgaacacat
     5101 aggcctgcag gaaagcgacc agaagttcaa gtgcggtgag agcgacggtc agcgccagcg
     5161 gcaacatgcc gccgatccag ccgatggcgc cgagcgaggt gccgagcatg gcgacgaacc
     5221 ccgcgaatac cttgagcgtg atatgcccgg ccagcatgtt cgcgaacaga cgcacgctgt
     5281 gcgagagcgg ccgcgagaag aaggagatga tttcgatcgt gacaacgagc ggcaggatgt
     5341 agatcggaac gcccgacggc acgaagatct tgaagaattt catgccgttt ttatagaagc
     5401 cgtacatcag caccgtgaag aacacgagca gtgcgaacgt ggccgtgata atgatatggc
     5461 ttgcgacggt gaaggtgtag ggaatgatgc cgaccaggtt cgagaccagg atgaacataa
     5521 acagcgagaa cacgagcggg aagaacctca tgccttccgc gccggcgttg gagcggatca
     5581 tgctggccac gaactcgtag ctcacttcgg ccatcgactg gaaacggccg gggaccagtt
     5641 gccggcctgc aacgcctccg atcatcagca ggcagatcac cgccaccgcg accagcatgt
     5701 aggccgacga attggtgaag tagatcggtt gaccgccgat gtggccgatc gtgaagaggt
     5761 tgtcgatatt gaactggtgg atcgggtcga gggtcatgcg cggctcttgt ccaccggctc
     5821 aagccggcaa gcaaaattca gatgtcgtga cttccgtcgg cgctagcgat cgcgaggaac
     5881 gacgccagcg gacctcacca cgttgaccac cccggcgacg aagccgagca gaaagaacac
     5941 gatgaaaccg aatggcgacg tcgacagca
//
LOCUS       DATLJT010000430         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2322041,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000430 DATLJT010000000
VERSION     DATLJT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322041"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VM910_09925"
     CDS             <1..650
                     /locus_tag="VM910_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62885.1"
                     /translation="FDVTSDFSPIAMVANSPLVLFAHPSVPFHDLKELIAYSKANPRK
                     LSVGTPGAGTPHHLAAAWLNTAAKIDITHVPYRGAAPALNDLLGGQIPLIWASPVAVM
                     PFVEQGKVKMLAVSTQQRDRMLPQVPTVSESGVPGFDTANWFGVVAPARVSSEVVARV
                     GQAIREASELPDVEKRMSTLGFNIDFRNSDQFREMIVRDHQKYGTIIREAGIQPD"
     gene            689..1063
                     /locus_tag="VM910_09930"
     CDS             689..1063
                     /locus_tag="VM910_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62886.1"
                     /translation="MHPVISMAPTANTSGQAAAVDFASRIVPLWQAALGPELLGYLRT
                     LLYPGRFCYSWMTGRMGSNDEAVAFLGRTRPAQLDVSLITRALQCRQADADPDSLFSA
                     RTTLPSQVDACAALVAGVAPQG"
     gene            complement(1156..1860)
                     /locus_tag="VM910_09935"
     CDS             complement(1156..1860)
                     /locus_tag="VM910_09935"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HVI62887.1"
                     /translation="MLGDPIFWLFVLPGLLLGAYAQSRIKINIAKYSQVGTQDGITGA
                     QVARRLLDSQGLRNVAVESTPGMLSDHYDPRSKTLRLSQEVYFAPSVAAAGIAAHEAG
                     HALQDAVDYFPLEARTYIVPVVQLASQTAPWLFIAGVMLQLQTLAWVGVILFGASSVF
                     ALITLPVEFNASARAQELLMSQGIIRGDEQIAGVQKVLGAAAWTYVAAAVSAIGSWLF
                     YVFVLLSQARSVGFRR"
     gene            2253..2741
                     /locus_tag="VM910_09940"
     CDS             2253..2741
                     /locus_tag="VM910_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62888.1"
                     /translation="MPNKYAEVTPRNFNPMWAPGLSDEARKAVNAAFDAMSTWRIETA
                     KSSEKNSEQVIEKMAAAARALGWPEQIVDTIRAQMQSITKMQIQTMDQMMDAWEEQIK
                     SPNPMTGSPSVMLSKLKSLPGVSPAGSWPNASALATNPMQFWMQWAEQWQKAWAEAMR
                     RH"
     gene            complement(2806..3363)
                     /locus_tag="VM910_09945"
     CDS             complement(2806..3363)
                     /locus_tag="VM910_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HVI62889.1"
                     /translation="MEHAKPIAGQTVLLQGTGGVSIFGLQLARMMGIQVIITSSSDDK
                     LARAKALGANHGINYQTTPDWEKAALGFTGGRGVDHIVEVGGAATLARSFGAIRVGGK
                     ITMIGGLSGPATELNPGLIFARRANVQGISVGSMQMFEALGRAMAASGIKPVIDKMFP
                     FDKARAGYQHMASGAHFGKIVIRVA"
BASE COUNT          774 a         1163 c         1054 g          698 t
ORIGIN      
        1 ccttcgatgt gaccagcgat ttctcgccga tcgcgatggt cgcaaattcc ccactggttc
       61 tgttcgcgca cccctcagtc ccgtttcacg atctcaagga attgatcgcc tatagcaagg
      121 ccaatccccg gaaactctca gtcgggaccc cgggcgccgg gacgccgcat caccttgcag
      181 ctgcatggtt gaacacggcg gcgaagattg acatcacgca tgtgccttac cgcggggcgg
      241 cgccggcact caatgacctg ctgggcgggc agattccgct catctgggcg tcgccggttg
      301 ccgtcatgcc gttcgtcgag cagggaaaag tgaagatgct ggcggtttcg acgcagcaac
      361 gagaccggat gctaccgcaa gtgccaacgg tctccgaaag cggtgtgccg ggatttgaca
      421 ccgcgaactg gtttggcgtc gtcgcgccgg ccagggtgtc atcggaagtc gtcgcccgcg
      481 tcggtcaggc cattcgtgag gccagcgagc ttcccgacgt cgagaagcgt atgtcgacgc
      541 ttggatttaa tatcgatttt cgcaatagcg accaatttcg cgaaatgatt gtcagggacc
      601 atcagaaata cggcaccatc attcgtgaag ctgggattca gccggattga agctgggagc
      661 ccagttcctc ggttctcgcg ggccgttcat gcaccctgtc atttccatgg ctccaacagc
      721 gaacacctcc ggccaagcgg ccgcggtcga ttttgccagt cgcattgtcc cattgtggca
      781 ggcggcgctc gggcccgaat tgctcgggta tctgcggacg ctcctctatc ccgggcggtt
      841 ttgctacagc tggatgactg gccgtatggg ctcgaacgac gaggcggtgg cgtttcttgg
      901 cagaacacgc cccgcccaac tcgacgtgag tttgatcacc cgcgcgctgc aatgtcggca
      961 ggccgacgcc gatcccgact ctctattttc tgcgcgaacg acgctgccgt ctcaggtcga
     1021 tgcctgtgcg gcccttgtcg ctggggtggc tccccagggt tgagcaccgc gctcgcgcca
     1081 gttcggagtc tcttcactag tcgttaattg cgcatcctgc tccgccgcgt cgcgtcgcag
     1141 cgctcgcgga ccaagtcatc tgcgaaaacc aacggagcgt gcctgggaaa gcagaacaaa
     1201 gacgtagaat agccagctac cgattgcgga gacggccgca gccacatagg tccaggcggc
     1261 agctcccaaa accttctgaa ctccagctat ttgctcgtcg ccccggatga taccctggct
     1321 catcaacaat tcttgcgctc gcgcactagc attgaattct actggcagag taatcaaagc
     1381 aaagacagaa gacgccccga agagaatgac gccgacccag gcgagcgtct gaagctgcaa
     1441 catcactcct gcgataaaga gccagggggc agtctgcgaa gctagttgca ctaccggcac
     1501 gatgtacgtt cgcgcctcca aggggaagta atccaccgca tcctgcaatg cgtgccctgc
     1561 ttcatgagcc gctattccgg ccgccgctac gctcggtgca aagtatactt cctggctcag
     1621 ccgcaatgtc ttggaacgag gatcgtaatg atcgctcagc atccctggag tggactcgac
     1681 tgctacgttg cgaaggccct gcgaatccag caaccggcgc gccacttggg cgccagtgat
     1741 tccgtcctgg gtgccgacct gagaatactt cgcgatattg attttgatgc gagactgcgc
     1801 gtacgcgccc aacagcagac ccggaagaac aaatagccaa aaaattgggt ctcctaacat
     1861 ttcgcgatct ctcgagtaag tggtgcccaa ggcaagggta gctcaaggtt tggcctctga
     1921 cacgtcgcaa cctgtcaatc agcgccccaa aattgaccca tcctctcacg gtcgaaatca
     1981 tccctgacag cctctcagtt gcgacaggcc cgcatcatag atcgacaggt tccgatagaa
     2041 caaaaaagcg agtctcggtc gcggcggttt cgcgctcagc gtcgtcattt taccaccagc
     2101 cctatcaatg cctgcgcagc cattgcgact gtcatgtctg catcacacgc catgcgatag
     2161 agtagccacg gtgatacctc agaacgctgg atgccttcgt ctactaggct ctccgggtag
     2221 attcttacaa tcagtgacag ggagacatgg ccatgccgaa taagtacgcg gaggtgaccc
     2281 cccgcaattt caatccgatg tgggctcccg ggctttcgga tgaggcgcgc aaggcggtga
     2341 acgcagcctt tgacgcgatg tccacttggc gcatcgagac cgccaaaagc agcgagaaaa
     2401 acagcgagca agtcatcgag aagatggctg ccgccgcccg ggcgctggga tggccagaac
     2461 aaatcgtcga taccattcgc gcgcaaatgc agagcatcac caaaatgcag atccaaacca
     2521 tggatcagat gatggatgct tgggaggagc agatcaaatc accgaacccg atgaccggct
     2581 cgccgtcggt gatgctgtcg aagctaaagt ccttgcccgg cgtcagcccc gccggcagtt
     2641 ggccgaacgc aagcgcgttg gcgacgaacc ccatgcagtt ttggatgcaa tgggcggagc
     2701 agtggcagaa agcctgggcc gaggcgatgc gccgccacta atcctcgaga gccgtgccgc
     2761 cgcgacctat tcctggcccg cccggcgtcg ggaatgagat cctgctcacg ctacgcggat
     2821 aacgattttg ccaaaatgtg cgcccgaggc catgtgctgg tagcccgccc gcgccttatc
     2881 gaaggggaac atcttatcga tgaccggctt aatgccgctc gccgccatcg cacgacccaa
     2941 cgcctcaaac atctgcatcg agcccactga gatgccctgc acgttggctc gacgggcaaa
     3001 gatcagtccc ggattgagtt cggttgcagg accgctcagc ccgccgatca tagtgatctt
     3061 gccaccgacg cggattgcac cgaacgagcg ggcgagcgtc gcagcaccgc cgacctcgac
     3121 aatatggtca acgccacggc cgccggtaaa tccgagcgca gccttctccc agtcgggcgt
     3181 ggtctggtag ttgatgccgt gattggcacc gagtgccttc gcacgcgcga gcttgtcatc
     3241 gctcgacgac gtaatgatca cctgaatgcc catcatgcgc gcgagctgga ggccgaagat
     3301 cgagacgcca ccagtgccct gcaacaacac cgtctgaccg gcgatcggct ttgcgtgctc
     3361 catcatcgca ttccaaacgg tgacgcccgc acaggggagg gtcgcgatcc tccagcgaca
     3421 agtgcgccgg aatttttacg accccgtcct cctccaacac cgcatattcc gcaagcgtgc
     3481 cgtcgatgcc gcctccaagc gcacttgcgt ggacgtcggc aggcgcctcg ccaccggccc
     3541 agcgctggaa gaaagcaccc ggcgacacgg tcgcctacct tcacgcgggt ggcaccggga
     3601 ccgatctcga tgacttcccc cgccccatcc gatagcggaa tgaggttctc gcgcaccggc
     3661 atccggtagg taccgcgcac aatgccgag
//
LOCUS       DATLJT010000431         6133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2322181,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000431 DATLJT010000000
VERSION     DATLJT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322181"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VM910_09950"
     CDS             complement(<1..309)
                     /locus_tag="VM910_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVI62890.1"
                     /translation="MTVVLAGCDQKPPQAAAAAPAVTVAQPVKRTVTDWDEFTGRFDA
                     IEEVQVRARVGGFVTNVEFKDGDMVHAGDLLYLIDSRPFEAVAAQADGQLADARAKVE
                     L"
     gene            688..2469
                     /locus_tag="VM910_09955"
     CDS             688..2469
                     /locus_tag="VM910_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI62891.1"
                     /translation="MDALSTRHGFPSEKARDRGPAYLAADCSEHNFYAIDRGLRDLLP
                     LYLAPRDFRALEPHFDRLGRLAGGRLDELARIADKHPPVLNPRDRFGRDEDWIDYHPS
                     YREMETIAFGDFQFHAMSHRAGTLGMDRPLPAVAKYALQYLFVQSEFGLMCPISVTDT
                     SIHLIRKFADQELKDYLLPKMLSGGCATMWKGTQFMTERAGGSDVGAIETMARHEDGE
                     WRLYGDKWFCSHADADVALLLARPEGAPTGTAGLALFALPRRLKDGSRNAFRIVRLKD
                     KLGTRSMASGEILLEGAVAYLVGDARAGLKQMMEQVNLSRLSHGVRAAAMMRRCLNEA
                     MVSAQSRIAFGKTIIEYPLLRRQLLKITLPAEQSLSMFLFAAHAMDRANAGDTDAALR
                     LRILTPLLKFRACRDNIPVATGAMEVRGGNGYIEEWVNARLIRDAHIGVLWEGTSNIN
                     ALDIVTRAVGRSRAHRALAAELDKLLGEAETIPAGFRNRLRDRLESALDFAERVAAEP
                     ALEANARAAASALYHATSAVVMTWEASRPGNDARRALLARLVLDMKLSAKDPLQPSRR
                     DWEREGAELIFAGRKIALAELAGLLDG"
     gene            complement(2470..3465)
                     /locus_tag="VM910_09960"
     CDS             complement(2470..3465)
                     /locus_tag="VM910_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVI62892.1"
                     /translation="MPPPEPLLQPTPTLSAVAELAGVSAITVSRVVRLPELVAPQTRA
                     RVEAAMRQLGYVPNQIAGALAGARTKSVGVLVPTIANSIFADTVQGLSDELEPLGYAV
                     ILAQSRYDAAREDHMLSALLSRRPEAIVMVGSPATEDGAWLLRRASIPVVETWELPAE
                     PIDAVAGFDNFAAGVAVAKHLVAQGRQHLAFIGGDDPRATRRWNGFSETVLRAGLRAP
                     RRLVLERNASGSLAALAELPGVDAVFAANDAHAIGFLSGLRNAGLLRDGPAAAQPVAV
                     VGLGDLEMGRLIAPRLSTIRVHGDAIGRTAAKLMLTRDGPRRVDLGFELVLRESG"
     gene            3582..4451
                     /locus_tag="VM910_09965"
     CDS             3582..4451
                     /locus_tag="VM910_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVI62893.1"
                     /translation="MISEADVAAYRRDGVIVVPEVVDRETLTRVRQVIAELVAGAAEV
                     SEHTDVYDLEPSHTRENPRVRRIKTPHKVHPIFNEIVRSQKVIDILTKLIGPGLRLHG
                     SKLNMKSAHYGSPVEWHQDWAFYPHTNDDVLAIGVLLDDCDLENGPMLVTPGTHTREV
                     WNHHGEDGYFAGLIDPDLIRDEIARAVPCIGKAGSMSFHHVRALHGSALNTSNRPRNL
                     LLYEVAASDAWPLMGVKDFDEFNSRLLTGEIIITPRVTEVPVRMPLPPAQHQGSIYEN
                     QSAAKKSYFTRAA"
     gene            4550..5899
                     /locus_tag="VM910_09970"
     CDS             4550..5899
                     /locus_tag="VM910_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62894.1"
                     /translation="MTEISADKAGQSRLRVIFAACIGSALEWYDFFLYGTAAALVFGE
                     LFFPKSDPMIGTLLAFLTFGVGFVVRPIGGILFGVLGDRFGRKPVLVATLLMIGIGTT
                     AIGCLPTYAQIGYWAPLLLVAMRVIQGLGAGAEYGGAVIYLVENAPAHRRGFWGSFAP
                     LGVSIGNLLAAGAFALVTPLPREALMSWGWRLPFLASFLLILVGIFVRLRVAETPVFT
                     EAVVARGKVEQNPAMEALRRHPRNFMVVLGARLAENGLGYLFPVFGLSYVITTLGVPK
                     SDALSALMLAFFIELFAIVGFASLSDRVGRRPVYLFGALAGVAFAFPFFLMVGTKQWI
                     WIALAFVIARAVVTAAMFGPQAAYFAELFPPQRRFAGFAFARELGSLLAGGPAPFLAA
                     YLVAVSGSWWPVACYAVLLSGLTAIAIWAGPETYTEDIAADEGADASMPAGIAPARA"
BASE COUNT         1051 a         1987 c         1987 g         1108 t
ORIGIN      
        1 aagctccacc ttggcacgcg cgtccgcaag ctggccgtcg gcttgcgcgg cgactgcttc
       61 gaacgggcgg gagtcgatca ggtaaagcag gtcgccggcg tgaaccatgt cgccgtcctt
      121 gaattcgacg ttggtgacga acccgccgac ccgcgcgcga acctgaacct cttcgatcgc
      181 atcgaaccgc ccggtgaact cgtcccaatc ggtcaccgtg cgcttcaccg gttgcgcgac
      241 cgtgaccgcc ggcgccgcgg cggccgcctg cggcggtttt tgatcgcagc ctgcgagcac
      301 gaccgtcaga acgatcaagg gcgcgatgcc ggcaaagcgg cgaacgccgg cggtttggtt
      361 gaaaagctcc gatgggtctg tcgcgctcaa ggagatctcc tctgcaccac acaagcgaca
      421 gggcgggcgc cctgcggttc ggaacgaaca agatgagacc tgccggcgtc cggttcaaga
      481 ccgggctggt ctcgccggtc tttccatata tcgggtttgt ggcgcaaatt ttttgccgcc
      541 gggccgacga tgatgggata atcgcactga gggcgatact gccctgatgg gcagcttgca
      601 ttgtgcggcc gcgcatgagg ccgccgccgt tcccggtcta tgctttgcga tgttcccttt
      661 tccaaatcat gcgctcggag gtcagccatg gacgcgcttt ccacccgcca cgggttccct
      721 tcggagaaag cccgcgatcg cggcccggcc tatctcgcag ccgattgctc agagcataat
      781 ttttacgcaa tcgatcgggg cctccgcgat ctgctgccgc tttatctcgc gccacgggat
      841 tttcgcgcgc tggagccgca cttcgatcgc ctgggcaggc ttgccggcgg tcggctcgac
      901 gagctcgcgc gcatcgccga caaacatccg ccggtgttga atccgcgcga ccgcttcggc
      961 cgcgacgagg actggatcga ttaccatccc tcctatcgcg agatggaaac aatcgcgttc
     1021 ggcgatttcc agttccatgc catgagccac cgcgcgggca cgctcggcat ggatcgtccg
     1081 ctgccggcgg tcgccaaata tgccctgcaa tatctgttcg tgcagtccga gttcgggctg
     1141 atgtgcccga tcagcgtcac cgacacgtcg atccatctca tccgcaaatt cgccgatcag
     1201 gagctcaagg actatctcct gccgaaaatg ctgtccggcg gctgcgccac catgtggaaa
     1261 ggcacgcagt tcatgaccga gcgcgccggc ggctccgatg tcggagccat cgagacgatg
     1321 gcgcgccacg aggatggcga gtggcggctc tatggcgaca aatggttctg ctcccacgcc
     1381 gatgccgatg tggcgctgtt gctggcacgc ccggaaggcg cgccgacggg cacggcgggg
     1441 ctcgcgctgt tcgcgctgcc gcgccggctc aaggacggaa gccgcaacgc ctttcgcatc
     1501 gtgcgcctga aagacaagtt gggaacccgc tcgatggcaa gcggcgaaat cctgctggag
     1561 ggcgccgtcg cctatctggt gggcgacgcc agggccggcc tgaagcagat gatggagcag
     1621 gtcaacctgt cgcggctgtc gcatggcgtg cgcgcggccg cgatgatgcg gcgctgcctc
     1681 aacgaagcga tggtcagcgc gcaaagccgc atcgcgttcg gcaaaacgat catcgaatat
     1741 cccctgctgc gccgccagct tctcaagatc acgctgccgg ccgaacagtc actctcgatg
     1801 tttctgtttg cggcacacgc gatggaccgc gccaatgccg gcgacaccga cgccgcgctg
     1861 cgtttgcgca tcctcacgcc gctgctcaag tttcgcgcct gccgcgacaa tatcccggtt
     1921 gccaccggcg ccatggaagt gcgcggcggc aacggctata tcgaggaatg ggtcaatgcg
     1981 cggctgatcc gcgatgccca tatcggcgtg ctctgggaag ggacatccaa tatcaacgcg
     2041 ctcgacatcg tcacgcgcgc ggtcggaaga agccgcgcgc accgcgcgct tgctgcggaa
     2101 ctcgacaaat tgctcggtga agcagagacg atccccgcgg gcttccgcaa ccggctgcgc
     2161 gacaggctcg agagcgcgct tgatttcgcc gagcgcgtcg cagcggagcc ggcgctggaa
     2221 gcgaacgcgc gcgctgcggc gagcgcgctt tatcacgcaa cgagcgccgt cgtgatgacg
     2281 tgggaagcaa gccggccggg caacgacgcc cggcgcgcgc tcctcgcacg cctcgtgctc
     2341 gacatgaagc tgagcgcaaa ggatccgctt cagccgagcc ggcgcgattg ggagcgcgaa
     2401 ggtgcggagc tgatctttgc cgggcgcaaa atcgctcttg ctgagctcgc cggattgctg
     2461 gacggctgat cagccggact cgcgcaacac caattcgaag ccgagatcca cgcggcgcgg
     2521 cccatcgcgc gtgagcatca gctttgccgc cgttctgccg atggcatcgc cgtgcacgcg
     2581 gatggtcgaa agtctcggcg cgatcagccg tcccatttcg aggtcgccga gcccgaccac
     2641 ggccaccggt tgcgcggccg ccggaccgtc gcgcagcaaa ccggcattgc gcagcccgga
     2701 caaaaatccg atggcgtgcg catcgttggc ggcgaacaca gcatccacgc ccggcaactc
     2761 cgcaagcgcg gcgaggctgc ccgatgcatt gcgctccagt acgaggcggc gcggcgctcg
     2821 taaccccgca cgcagcaccg tctcgctaaa tccattccag cgtcgcgtcg cgcgcggatc
     2881 gtcgccgccg atgaaagcga ggtgttgccg tccttgtgcg accagatgct ttgcgaccgc
     2941 aacgccggct gcgaaattgt cgaagcctgc gaccgcatcg atcggctcgg cgggcagttc
     3001 ccaggtctcc accacaggga tacttgcgcg ccgcaacagc cacgcgccgt cctcggtcgc
     3061 cggcgatccc accatgacga tcgcctccgg ccggcgcgac aaaagcgcgg acagcatgtg
     3121 gtcctcgcgc gcggcgtcgt agcgcgattg cgccaggatc actgcatagc cgagcggctc
     3181 cagctcgtcg gacaagccct gtacggtgtc ggcaaaaatc gaattggcga tggtcggcac
     3241 cagcacgccg accgacttgg tgcgtgcacc agcgagagcg ccggcgatct ggttcggcac
     3301 atagcccaac tgtcgcatcg ccgcctcgac ccgggcgcgg gtctgcggcg caaccaattc
     3361 gggcagccgc accacgcggc tcacggtgat ggcggaaaca ccggcaagct ctgcaacggc
     3421 agagagggtc ggggtcggct gcagcagggg ttccggaggc ggcatcgttt ggctcgaaag
     3481 ggtctcgata caccaaagcg cttgcgggcg catggtgata gcgctaacat cacaaatgtt
     3541 agcgctatca tatacgaaaa taacgggatg cggagtgctc aatgatttct gaagcggatg
     3601 tcgcggccta tcggcgggac ggcgtgatcg tggttccgga ggtcgtggat cgcgaaacgt
     3661 taacgcgggt gcgacaggtg atcgccgagc ttgttgccgg cgcagccgag gtgtccgagc
     3721 acactgacgt ctacgatttg gaaccaagcc acacccgcga aaatccgcgc gtgcgccgga
     3781 tcaagacgcc gcacaaggtg catccgatct tcaacgagat cgtccgcagc cagaaggtga
     3841 tcgacatcct gacaaagctg atcggcccgg ggctcaggct gcacggctcg aagctgaaca
     3901 tgaagtcggc gcattacggc tcacccgtgg agtggcatca ggactgggcc ttctatccgc
     3961 acaccaatga cgacgtgctc gcgatcggcg tgctgctgga cgattgcgac ctcgagaacg
     4021 gtccgatgct ggtgacgccg ggcacgcaca cccgcgaggt ctggaatcac catggcgagg
     4081 acggttattt tgccggcctg atcgatcccg acctgatccg cgacgagatc gcgcgcgccg
     4141 taccctgcat cggcaaggcg ggcagcatgt cgttccatca tgtccgagcg ctgcacggct
     4201 cggcgctcaa cacctcgaac cgtccgcgca acctgctgct ctatgaagtc gcggccagcg
     4261 acgcctggcc tttgatgggc gtgaaggact tcgacgagtt caacagccgc cttttgaccg
     4321 gcgagatcat catcacgccg cgggtcaccg aggtgccggt gaggatgccg ctgccgccgg
     4381 cacagcatca gggctcgatc tacgaaaacc agtcggcggc gaagaagtcg tacttcacta
     4441 gggccgcttg atggggcaag ccgcgcggcc cgcgaaagcg ggctgcacat cgtcgccttc
     4501 gaagatcaat cagacaaaac aatgacaaca aatcctacag ggggaaacca tgacggagat
     4561 ttcagccgat aaagcgggcc aatcgcggtt gcgcgtgatt ttcgcggcct gcatcggatc
     4621 cgcgctggag tggtacgact tctttctcta cggcaccgcc gccgcgctgg tgttcggcga
     4681 gctgttcttc ccgaagagcg atccaatgat cggcacgctg ctcgccttcc tgacctttgg
     4741 cgtcggcttc gtggtgcggc cgatcggcgg cattctgttc ggcgttttgg gcgaccgctt
     4801 cggccgcaag cccgtgctgg tcgcaacgct gcttatgatc ggcatcggaa cgacagcgat
     4861 cggatgtctg ccgacctatg cccagatcgg ctattgggcg ccgctgctcc tggtcgcaat
     4921 gcgggtgatc caggggttgg gcgcgggcgc cgaatatggc ggcgcggtga tctatctggt
     4981 cgagaacgcg ccggcgcatc ggcgcggctt ctggggcagc tttgcgccac tcggcgtctc
     5041 catcggcaat ctgcttgccg cgggcgcctt tgcgttggtg acgcccttgc cgcgcgaagc
     5101 cctgatgtcg tggggctggc ggctgccgtt cctcgcaagt ttcctgctga tcctggtcgg
     5161 tatcttcgtc cgcctgcggg ttgcggaaac tcccgtcttt accgaagccg tggtcgcgcg
     5221 cggcaaggtg gagcagaacc ccgcgatgga agcgctgcgc cggcatccgc gcaacttcat
     5281 ggtcgtgctc ggcgcgcgcc tggccgagaa cggcctcggc tatttgtttc cggtgttcgg
     5341 cctgagctac gtcatcacca cactcggcgt tcccaaatcc gatgcgctga gcgcgctgat
     5401 gctcgccttc ttcatcgagc tgtttgccat cgtcggcttt gcctcgctct ccgaccgggt
     5461 cggtcggcgg ccggtctacc tgttcggcgc gctggccggc gtcgccttcg cgttcccgtt
     5521 cttcctgatg gtcggcacca agcaatggat ctggatcgcg ctggctttcg tcatcgcgcg
     5581 cgccgtggtg acggccgcga tgttcggccc gcaggctgcg tattttgcag agctgttccc
     5641 gccgcagcgg cgctttgccg gctttgcctt cgcgcgcgag ctcggttcgc tgctcgccgg
     5701 tggacctgca ccgttccttg cggcttacct cgtggcggtg tccggaagct ggtggccggt
     5761 cgcctgctat gcggtgctgc tgtccggcct gaccgcaatc gcgatctggg ccggtcccga
     5821 gacatatacc gaggacatcg cagccgacga gggcgcggat gcttcgatgc ccgcagggat
     5881 cgcaccggcg cgggcctgac gatgaagttg cgtgcagacg cggcaggagc ccgcaagcgc
     5941 ttgcaggctc tgacctgttc ggctgcgacc aaccggtatg ccgatgcgcg ccatcttcgg
     6001 gcatgacgcg accaatttcc ttcgctagtc tggcctcaaa agcaaaaacc aatttaccgg
     6061 gaggacaagc gtgcacagag gacgtgtcgt cttcggcgcc atggacgagg tcgtgttcgg
     6121 tgaggcggcg gct
//
LOCUS       DATLJT010000432         1713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1403149,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000432 DATLJT010000000
VERSION     DATLJT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403149"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VM910_09975"
     CDS             <1..1116
                     /locus_tag="VM910_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI62895.1"
                     /translation="AVVGVGHTRYGKLPEYDAYELGLWALKEALADSGLRFEDIDGLI
                     VNRIPDYQRFCEIAGLNPSYVSTTPGQGRFSGICIETAAALIRDRLATIVALVYGNNG
                     RSGGDRYGGAGDTYGSGGGGQWFAYGMTSPGAFHALMARRHMDLYGTTADQLGAVAKT
                     FRDHAALNPSAVMRTPFTLTDYHASRYICEPLHLLDYCLINDGGVALILTSGERARDL
                     AQRPVHVRGCGMATALAPSTFPPDDFWREPMRKAAAMSFRAARASHDDISALMIYDNF
                     TPTVLLSLEGFGYCGEGESGPFAAEGHLALGARYPANTSGGHLSESYMQGWALNLEAV
                     LQIRGTCGARQVKDCAFAHYMAAAPVVTSIIYGRDPS"
     gene            1113..1514
                     /locus_tag="VM910_09980"
     CDS             1113..1514
                     /locus_tag="VM910_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVI62896.1"
                     /translation="MRPLPNVDGPDQPFWRALRCGEVRVQRCGNCGLCRFPATRFCPQ
                     CRSETSEWHPVAPIGIIETWCIFHRPYFDGLGVPYTVIQVRLDCGVRLFSNPVGVEAG
                     SLHIGMPVEGVFEDITPDVTLLKFKPRTEYR"
     gene            1511..>1713
                     /locus_tag="VM910_09985"
     CDS             1511..>1713
                     /locus_tag="VM910_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI62897.1"
                     /translation="MKAIVVRAPGGTEVLKIEQILDPTPGPKDVIIKIDACGVCFHDV
                     VTRNGTLKFGVQMPCILGHEISGT"
BASE COUNT          305 a          527 c          533 g          348 t
ORIGIN      
        1 gccgtggtcg gcgtggggca cacacgctac ggcaagttac cggaatacga tgcctatgaa
       61 cttggtttgt gggcgctgaa ggaggcgctg gcggattccg gccttcgttt cgaggatatc
      121 gacggactaa ttgtcaaccg cattcccgac tatcagcgct tctgcgagat cgcaggattg
      181 aacccgtctt acgtgagcac aacccccggt cagggccgct tctcgggcat ttgcatcgag
      241 acagccgccg cgctcattcg cgacaggctc gcgacgatcg tagccctcgt ctacggaaac
      301 aacggccgct cgggcggaga tcgttacggc ggggcaggtg acacctacgg cagcggcggc
      361 ggcggccaat ggtttgccta cggcatgact tcccccggcg cattccatgc gctgatggcc
      421 cgccgccata tggaccttta cggaacgact gcggatcaac tcggcgctgt cgcgaagacg
      481 tttcgtgacc acgcggcgct caatccttcg gccgtgatgc gcactccctt caccctcacc
      541 gactatcacg cgtcgcgcta catctgcgag ccgctgcatc tgctcgacta ttgtctcatc
      601 aacgacggcg gcgtagcgct gatcctcact tccggcgagc gagcgcgcga cctcgcccaa
      661 cgtcccgtcc acgtgcgtgg ttgcggcatg gcgacggccc ttgcgccctc gacttttcca
      721 cctgacgatt tctggcgcga gcccatgcgt aaggcggcgg ccatgagctt ccgcgcggcc
      781 agagcgagtc acgacgacat atcggctctc atgatctacg acaactttac accgaccgtg
      841 ctcctctcgc tcgagggctt cggctattgc ggtgagggtg agagcgggcc gttcgcggct
      901 gaaggtcatc tcgcgcttgg tgcgcgatat cctgcaaata catccggtgg ccatttgtcc
      961 gaatcctata tgcaaggctg ggcgctcaac cttgaggcgg tgctgcaaat taggggcacg
     1021 tgcggcgcgc gccaagtgaa ggactgcgcg ttcgcccatt atatggcagc cgcaccggtc
     1081 gtgacgtcga tcatctatgg cagagatcca tcgtgagacc gctcccgaac gtcgacggac
     1141 cggatcagcc gttctggcgg gcgctgcgct gcggcgaggt gcgcgtccag cgctgcggaa
     1201 attgcggctt gtgccggttt cccgccacgc ggttctgccc gcagtgccgg tcggagacat
     1261 ccgaatggca tccggtcgca ccgattggca tcatcgagac atggtgcatt tttcatcgac
     1321 cctacttcga tggccttggc gtgccatata ccgtgatcca ggtgcgactc gattgcggcg
     1381 tccggctgtt ctccaatcct gtgggagtcg aggctggctc gctccatatc gggatgccgg
     1441 tggagggcgt gttcgaggac atcacgccgg acgtgaccct gttgaagttc aagccgcgga
     1501 cggaataccg atgaaagcga ttgtggtgcg cgcgcctggc ggcactgagg tcctcaagat
     1561 cgaacaaatt ctcgatccga cgccggggcc aaaagatgtg atcattaaga tcgatgcgtg
     1621 cggcgtgtgc tttcacgacg tggtcacgcg caacggtacg ctgaaatttg gcgtgcagat
     1681 gccgtgcata ctgggacacg agatctccgg aac
//
LOCUS       DATLJT010000433         7833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2782403,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000433 DATLJT010000000
VERSION     DATLJT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782403"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="thiC"
                     /locus_tag="VM910_09990"
     CDS             <1..1360
                     /gene="thiC"
                     /locus_tag="VM910_09990"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVI62898.1"
                     /translation="SEIARGRAIIPSNINHGELEPMIIGRNFLTKINANIGNSAVTSS
                     VEEEVDKMVWAIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKVNGD
                     PVKLDWEVYKDTLIEQCEQGVDYFTIHAGVRLPYIHLTANRVTGIVSRGGSIMAKWCL
                     AHHKESFLYTHFADICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTKI
                     AWDKGCQVMIEGPGHIPMHKIKINVDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGI
                     GAAMIGWFGCAMLCYVTPKEHLGLPDRNDVKTGVITYKISAHAADLGKGHPAAQLRDD
                     ALSRARFDFRWVDQFNIGLDPDTAKAFHDETLPKEAHKVAHFCSMCGPKFCSMKITQD
                     VRDYAATLNDKEQKGFGAEGDQIPSSEVEKGMAQMSAKFREMGSNVYLDAEVVKESNK
                     AL"
     gene            complement(1504..2100)
                     /locus_tag="VM910_09995"
     CDS             complement(1504..2100)
                     /locus_tag="VM910_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVI62899.1"
                     /translation="MQIQFWFDFASPYAYVASERIETLARSHGATVDWCPLLLGVVLK
                     RKSADGSPFQRATEAEHRYRRRDIERLCIRNGLPLVWPTGFPRGSLLAMRTAYVAREQ
                     GWGREFANQVFRANFVHDRDIAKTEVVADLIEACKRPSGSVLELAQSEENKKAFLEHV
                     EQAIARGIFGVPTVVVGDELFWGNDKLEEAFEWLERER"
     gene            complement(2248..2688)
                     /locus_tag="VM910_10000"
     CDS             complement(2248..2688)
                     /locus_tag="VM910_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62900.1"
                     /translation="MTMTSAPRGALLALTTFAFTTLSTVCHAESGTVTLTIWKAGWII
                     GGSGGGGVLYFRGRAYPLSTGGLDYGLVFGGSKTVLHGTVSNIYRPSDVAGVYGAAGA
                     GLAIGGGARAIVLTNQKGAVLELSGRQVGLMANADLSGLAITMR"
     gene            2807..3271
                     /locus_tag="VM910_10005"
     CDS             2807..3271
                     /locus_tag="VM910_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI62901.1"
                     /translation="MAKQSAGLLMFRRRRGAIELLLVHPGGPYWARKDDGAWSIPKGF
                     VEEGEDTLVAAKREFEEETGQRPTGEFIALGVFKQPSGKQVSAFAVEGEFDLASFKSN
                     NFALEWPPRSGRMQEFPEADRAGWFTPQEALVKVTKGQRPIIQALLEGLGIG"
     gene            3335..3724
                     /locus_tag="VM910_10010"
     CDS             3335..3724
                     /locus_tag="VM910_10010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI62902.1"
                     /translation="MSEIKHFPPPAGIKAPPLSFATRTGDLLFISGIPGFDEKRELPD
                     NFEAQFKNVVANIKRVLDEAGGSFRDLVKVNVLLTRAGDVATMNALYAGAFGPPPYPA
                     RTTCVVQALPDPKMLIEIEGVASLQGR"
     gene            3943..4632
                     /locus_tag="VM910_10015"
     CDS             3943..4632
                     /locus_tag="VM910_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI62903.1"
                     /translation="MLTLYSYPTLFGVADNNGYGLKVFAFLKLAGVPFRHEHIFDASK
                     APRGQLPYIVDGNDTVGDSETILAYVIDKYRLTIDAALTAAQRAQNLLIARTLDDLYW
                     VMSYSRWKDERYWPLFRDALLREHPTLTEDGLAKAKEFNAQRYYYQGIGRYDPDAAMA
                     RGLADLAALSTLIPPRGYVHGGNPTSVDAGIYGFIANIYFYEIDTPLKAFVVAHDNIV
                     QHCRAIHEAVR"
     gene            complement(4629..5114)
                     /locus_tag="VM910_10020"
     CDS             complement(4629..5114)
                     /locus_tag="VM910_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62904.1"
                     /translation="MDTQLKNWELWIDRLWSAAAIDWHEAARLALDIASAAQDDTLRH
                     AASQGLPSLRNAALWSADDAAREAARRRLGVIREALHALTLPRFGKRDAAPASLSAEE
                     HYRQLLGLPLDRRLSAPEIHRAFKRAAKTAHPDAGGDARAFLELSAARDALMKQGIRY
                     R"
     gene            5367..6134
                     /locus_tag="VM910_10025"
     CDS             5367..6134
                     /locus_tag="VM910_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI62905.1"
                     /translation="MPLLDKHIAVITGAASGIGRAIAKGFAREGARVVLLDVNEQAAR
                     EVVKEIRDAGASAESFRLDVTRREDCFLVAKEIAAKVGQVSILVNNAGINRRNAFTAD
                     TEAVLKDWQDIMAINLNGVFNVTHAFLEPLKASKGRIVNIGSIQSFMHVRTPNSPAYT
                     TSKHAVLGFTRALAAELGKHGVRVNAIGPGLIETPLNASVRANDPGLVKIFIDHTPLG
                     RAGQPEDIVGPALFLASDLSSYVTGGIVMADGGYRTI"
     gene            complement(6189..6461)
                     /locus_tag="VM910_10030"
     CDS             complement(6189..6461)
                     /locus_tag="VM910_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HVI62906.1"
                     /translation="MCRNIKTLFNFEPPATEDEIQASALQFVRKLSGFNKPSQANEAA
                     FDRAVEEVSEAARRLLDSLHTHAPARDRAVEAEKARERSRLRFGRA"
     gene            6616..6861
                     /locus_tag="VM910_10035"
     CDS             6616..6861
                     /locus_tag="VM910_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HVI62907.1"
                     /translation="MSHFTYRIVQHDGGWAYTADGVFSESYATHAEALAAAKRVAAEQ
                     RAPGRTEIIEYETSDGKWHTETAEGSDRPETDVEESD"
     gene            complement(7127..7570)
                     /locus_tag="VM910_10040"
     CDS             complement(7127..7570)
                     /locus_tag="VM910_10040"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI62908.1"
                     /translation="MMLQKPLVQSGFSRVAEALSDPAREAIVSALSDGKAMPAGELAS
                     IAGISPQSASAHLQKLVDARVLSVLAQGRFRYYRIDDDEVAALIEGLVNLAAKTQSGG
                     RARRQVGDELRQLRSCYCHLAGQLGVSLGDTLLRRGFVELNGRAG"
BASE COUNT         1553 a         2531 c         2374 g         1375 t
ORIGIN      
        1 ctccgagatc gcgcggggac gggccatcat tccctccaac atcaatcacg gcgagctcga
       61 gccgatgatc atcggccgca acttcctcac caagatcaac gccaatatcg gcaattcggc
      121 agtgacctcc tcggtcgagg aggaggtcga caagatggtg tgggcgatcc gctggggcgc
      181 cgacaccgtg atggacctct ccaccggacg caacatccac accacgcgcg agtggatttt
      241 gcgcaactcg ccggtgccga tcggcaccgt gccgatctat caggcgctgg agaaggtgaa
      301 cggcgacccc gtcaagctcg actgggaagt ctacaaggac acgctgatcg agcagtgcga
      361 acagggcgtc gattacttca cgatccacgc cggcgtccgc ctgccctaca tccacttgac
      421 cgccaaccgc gtcaccggca tcgtctcgcg cggcggctcg atcatggcga agtggtgcct
      481 tgcccatcac aaggagagct tcctctatac gcatttcgcc gacatctgcg acctgatgcg
      541 gaagtacgac gtctccttct cgcttggcga cggcctgcgt cccggctcga tcgccgacgc
      601 caatgaccgc gcgcagtttg ccgagctcga gacgcttggc gagctcacga agatcgcctg
      661 ggacaaaggt tgccaggtca tgatcgaagg ccccggccac atcccgatgc acaagatcaa
      721 gatcaacgtg gacaagcagc tcaaggagtg cggcgaagcg ccgttctaca cgctgggacc
      781 gctgacgacc gacatcgcac cgggctatga ccacatcacc tcgggcattg gcgctgcgat
      841 gatcggctgg ttcggctgcg ccatgctctg ctatgtcacg ccgaaggagc atctcggcct
      901 gcccgaccgc aacgacgtca agaccggcgt catcacctac aagatttcgg cgcatgccgc
      961 cgatctcggc aagggccacc ccgctgcaca attgcgcgac gatgctcttt caagggcgcg
     1021 gttcgacttc cgctgggtcg accagttcaa catcgggctc gatccggaca ccgcaaaagc
     1081 cttccacgac gagaccctgc cgaaggaggc gcacaaggtc gcgcatttct gctcgatgtg
     1141 cggaccgaaa ttctgctcga tgaagatcac gcaggacgtg cgcgactatg cggccacgct
     1201 gaacgacaaa gaacaaaaag gatttggagc cgaaggcgat caaatcccgt cttcagaagt
     1261 ggaaaaaggc atggcgcaga tgagtgccaa gttcagggag atgggctcca acgtgtatct
     1321 cgatgcggag gtcgtgaagg agagcaacaa ggcgttgtga gctctcttcg cttctacgaa
     1381 cgcgaaaggc cgggcgaaag cccggccttt cttcatccgg catgcgtatc tctcgacatc
     1441 cggggcgacg acgcctcgca tatcgctatc gctcatggag ctacggcggc agagcgtgcc
     1501 tgcttagcgc tctcgttcga gccactcgaa cgcctcttcc agcttgtcgt tgccccaaaa
     1561 aagctcatcg ccgacgacaa ccgtcggaac gccgaatatg ccgcgcgcga tcgcctgctc
     1621 gacatgttcc aggaacgctt tcttgttttc ctccgactgg gccaattcca gcacgctacc
     1681 ggaaggccgc ttgcaggctt cgatcaggtc cgcgacgacc tcggttttcg cgatgtcgcg
     1741 gtcgtgaacg aaatttgccc gaaacacctg gtttgcgaat tcgcgacccc aaccctgctc
     1801 ccgcgcgaca tacgcagtcc gcatagccag caggctgccg cgcggaaagc cggtcggcca
     1861 gacgagcggc agtccgtttc ggatgcatag ccgctcgatg tcccgccggc gataacgatg
     1921 ctccgcttcg gtggcgcgtt gaaagggact gccgtccgcc gacttgcgct tgagcacgac
     1981 gccgagcagc aggggacacc aatcgactgt tgcgccgtgc gaccgcgcaa gcgtctcgat
     2041 tcgctcggag gcgacatagg cgtatggact cgcgaagtcg aaccagaact ggatctgcat
     2101 ctcgttctca caaaatcgtg gctaacgtgg ctcaagcgtt gttagcccaa attctgcgcg
     2161 acacgaatga aatccaatca atcccggttg tcgatttctc attcgcactt ggaactcacg
     2221 ccgcagctcc gcgcgggaga atcttcccta cctcatcgtg atcgcgagcc cgctcaaatc
     2281 cgcgttcgcc atcagcccaa cctgacggcc ggacaattcc agcaccgcgc ccttctggtt
     2341 ggtcagcacg atggcacgcg ccccgccgcc gatcgcaagt cccgcgcccg ccgcgccgta
     2401 gacgccggcg acatccgagg gccggtagat gtttgagacc gtgccgtgca gcacagtctt
     2461 cgatccgccg aacacgaggc cgtagtcgag cccgccggtc gacagcggat aagcccgccc
     2521 gcggaaataa agcacgccgc cgccaccgga accgccgatg atccatcccg ccttccagat
     2581 cgtgagcgtc acggtgccgc tctcggcatg gcagacggtg gacagcgtgg tgaaggcgaa
     2641 agtcgtcagc gcaaggagtg cgccgcgcgg ggcggatgtc atggtcatgt gatagtctct
     2701 cccggatgtg gtcggcattg ccaagccaaa cgaatcacct cggccgaatc tagccaatgg
     2761 tgcggggagg cgaaatgcat tcgccgatgc cgcataaggc gaaaacatgg cgaagcaaag
     2821 tgccggcctt ctgatgttcc gtcgccgccg cggggcgatc gaattgttgc tggtgcaccc
     2881 gggcggcccg tactgggcga ggaaggacga cggcgcctgg tcgatcccca aagggtttgt
     2941 cgaggagggc gaagacacgc tggttgcagc gaagcgcgag ttcgaggaag agacggggca
     3001 gcggccgacc ggcgagttca tcgcgctcgg cgtgttcaag cagcccagcg gcaagcaggt
     3061 cagtgccttc gcggtcgaag gggagttcga tctcgcatcg ttcaagagca acaattttgc
     3121 gttggagtgg ccgccgagat ccgggcgaat gcaggagttt ccggaagccg atcgcgccgg
     3181 ctggttcact ccgcaagaag ccctggtcaa ggtcacgaag ggacaacgcc cgatcattca
     3241 ggcgttgctg gaaggattag gaataggata gcgcctccgg aaagcttaag cttccatccg
     3301 tcgaaattcc tggaaaaaag ccccgaggta tccgatgtca gagatcaagc acttcccgcc
     3361 gcccgccggc atcaaggcgc cgccgctctc cttcgcaacg cgcacaggcg atctgctctt
     3421 catctccggc attcccggct tcgacgagaa gcgcgagctg cccgacaatt tcgaggcgca
     3481 gttcaagaac gtcgtcgcca atatcaagcg cgtgctggac gaggccggcg gcagcttccg
     3541 cgatctcgtc aaggtgaacg tgctgctgac gcgtgccggc gatgtcgcga cgatgaacgc
     3601 gctctatgcc ggcgcattcg gtccgccgcc ctaccctgcc cgcaccacct gcgtcgtgca
     3661 ggcgctgccc gacccgaaaa tgctaatcga gatcgagggc gtggcatcgc tgcagggacg
     3721 ctgacgcttc gccggcgggt ggccgtcggg ttacaaggtc atcagcaaac cggtcccgac
     3781 ccttggcgca gcgctcggtg tcaaggacga tttgctcaag cacggcaagt gcaggtacgg
     3841 caagtgcagg ttctgacaat ttaggacttg cctggatcga ggccgatggg cgaaaccttc
     3901 atcggccttt ttcattgatc gcaagaaccg aggtaggaac cgatgctgac gctctactcc
     3961 tatcccacgc tgttcggcgt cgccgacaac aacggctacg ggctgaaggt gttcgccttc
     4021 ctgaagctcg ccggcgtacc gttccgccat gagcatattt tcgacgcctc gaaggcgccg
     4081 cgcgggcaac tgccctacat cgtcgatggc aacgacaccg tcggcgacag cgagacgatt
     4141 ctcgcctatg tcatcgacaa ataccgcttg accatcgatg ccgcgctgac cgcagcgcag
     4201 cgcgcgcaaa acctcctgat cgcgcgcacg ctcgacgatc tctattgggt gatgtcctat
     4261 tcgcgctgga aggacgagcg ctactggcct ctcttccgcg acgcactgct gcgcgagcat
     4321 ccgaccctga ccgaggatgg tcttgccaag gcaaaggagt tcaacgccca gcgctattac
     4381 tatcagggga tcggccgcta cgatcccgat gccgcgatgg cgcgcgggct cgccgatctc
     4441 gcggcgctgt cgacgctgat cccgccgcgc ggctatgtgc atggcggcaa cccgaccagc
     4501 gtcgatgccg gcatctacgg cttcatcgcc aatatctatt tctacgagat cgacacgccg
     4561 ctgaaagcgt tcgtggtcgc ccatgacaac atcgtgcagc actgccgcgc gatccacgaa
     4621 gccgtacgct agcgatagcg gatgccctgc ttcatcagcg catcgcgcgc cgcggacaat
     4681 tcgaggaagg cccgcgcgtc gccgccggca tccggatgcg cggtcttcgc cgcgcgcttg
     4741 aatgcccgat gaatttctgg cgcgctgagg cggcggtcga gcggcagccc gagcagttgc
     4801 cggtaatgtt cctccgccga gagcgatgcc ggcgcagcgt cgcgcttgcc gaagcgcggc
     4861 agggtcaaag catgcagcgc ctcgcggatc accccgagcc gccgccgcgc ggcctcccgc
     4921 gccgcatcgt cggcgctcca caacgcggcg ttgcgcaggg aaggcaggcc ctgcgacgcg
     4981 gcgtgacgca acgtgtcgtc ctgggcggcg cttgcgatgt cgagcgcgag gcgcgcggcc
     5041 tcatgccagt cgatcgcagc cgccgaccac aggcggtcga tccagagctc ccagttcttc
     5101 aactgcgtgt ccatggcgca attctaagcg cccacgcgta aagaaacctt ggcgattacg
     5161 tcagtgttgc ggtttgcgaa gttctcttca tctctgacga gaatacttca agagaacgtc
     5221 cgaaccaaaa ctgcactggc atcatatatt tgctagagtt ctttcgagcc cgaagttcgc
     5281 agcgcaggca accgggttcg agacgctggt ttcaatttga atgaacttgt cgcggacgcc
     5341 gaaagacaca attagggagg actcaaatgc cgctactcga caaacatatc gccgtgatca
     5401 ccggggcggc atcgggcatc ggccgcgcga tcgcaaaagg ctttgcgcgc gagggcgcac
     5461 gcgtggtgct gctcgacgtc aacgagcagg cggcgcgcga ggtggtaaag gagatccgcg
     5521 acgcgggcgc gtccgccgag agctttcgcc tcgatgtcac ccggcgcgag gactgcttct
     5581 tggttgcaaa agagatcgcc gccaaggtcg ggcaggtctc gatcctcgtc aacaatgccg
     5641 gcatcaaccg ccgcaacgcc ttcaccgccg ataccgaagc cgtgctgaaa gactggcagg
     5701 acatcatggc gatcaatctc aacggcgtct tcaacgtcac ccacgccttc ctggagccgc
     5761 tgaaggccag caaggggcgt atcgtcaaca tcggctcgat ccagtccttc atgcatgtgc
     5821 gcacgccgaa ctcgccggcc tataccacgt ccaagcacgc ggtgctcggc ttcacccggg
     5881 cgcttgcggc cgagctcggc aagcatggcg tgcgcgtgaa tgcgatcggg cccggattga
     5941 tcgaaacgcc gttgaatgcg agcgtgcgcg ccaacgaccc cggcctggtg aagattttca
     6001 tcgaccatac cccgctcggc cgcgccggcc agccggagga catcgtgggg cctgccctct
     6061 tcctcgcctc cgacctgtcc tcctatgtca ccggcgggat cgtgatggcc gatggcggct
     6121 acaggacgat ctaggcgatg atcttttccg cgtcgtagcg agcgaacagc gccggcgcga
     6181 caatctctct acgcgcggcc gaacctgagc cgcgagcgtt cacgcgcctt ttccgcttcc
     6241 accgcgcggt cgcgtgccgg cgcatgggtg tgcagggagt ccaggagccg ccgcgccgct
     6301 tcggacacct cttccaccgc gcggtcgaac gccgcttcgt tggcctgcga cggcttgttg
     6361 aagccggaaa gctttcgaac gaactgcagc gcagaggcct ggatctcgtc ctcggtcgcc
     6421 ggcggctcga aattgaacaa ggtcttgata ttgcggcaca tgagctactc cctggcctgc
     6481 cctcgcacgt cgaacggccc aacgccgccc ctgtccaaat ggacgattac ctgacctaaa
     6541 atcctacatc ctcgcgcggt ttttgccata aaatagcgga cgcgcacggc gcgactgcga
     6601 acaggcgggt ttcccatgag ccatttcact tacaggatcg ttcagcatga cggcggctgg
     6661 gcctacaccg ccgacggcgt gttttccgag agctatgcga cccatgctga ggcgcttgca
     6721 gccgccaagc gcgttgccgc cgagcagcgc gcaccggggc ggaccgaaat catcgaatac
     6781 gagacctcgg acggcaaatg gcacaccgaa actgccgaag gcagcgatcg gcccgagacc
     6841 gacgtcgagg agagcgactg acaggcgttt ccgctcagaa cggaatcctc gcgccaagcc
     6901 ggtcgaagaa ggcacggccc cggggcgtca gtcgcagcgc ccgcggaacg tcgcgcggca
     6961 cgatacatcg cgtttccgtt agatgcccga agatcgcctt ggcaagcggg ccggagagat
     7021 ggggaacgcg ctcggtccag tcgttgcaga gccggcaatg cggggcgcgg gtcggctcga
     7081 aatcgatgcc ttccgacttg caccacgcaa gcccgctctt tgttgcttag ccggcccgcc
     7141 cgttgagctc gacgaacccg cgccgaagca gcgtgtcgcc cagtgagact ccgagttggc
     7201 ccgcgagatg acaatagcaa ctgcgcagtt ggcgcaattc atcgccgacc tgccggcgcg
     7261 cgcggccgcc gctttgcgtc ttcgccgcga gattgacgag gccttcgatc agagccgcaa
     7321 cctcgtcgtc gtcgatccgg taatatctga acctgccctg cgcgagcacc gagagcaccc
     7381 gcgcgtccac gagcttctgc aggtgcgcgc ttgcgctttg cggcgatatg ccggcgatcg
     7441 acgcaagttc gccggccggc atcgccttgc catccgaaag cgcgctgacg attgcctcgc
     7501 gcgcgggatc ggacagcgcc tccgcgacgc gcgaaaagcc cgactggacc aatggctttt
     7561 gcaacatcat tcggactccc gcgcgtgacg aagcgactat agccgatttc gggaaaaact
     7621 catttcagga tttgatgaaa catcactcct ccgcggcttg ccacgctggg cacgcttcca
     7681 tgcaatgaga ggaacatcat gaccagcgaa acagcgcgaa acgatatcga acgcatctac
     7741 aggctttggg atgaggctct gggcaagaag gacctggaag catcgctttc gctttacgcg
     7801 gatgacgcca cgatcgaaag tccgttggtt cgg
//
LOCUS       DATLJT010000434         4573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3930593,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000434 DATLJT010000000
VERSION     DATLJT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3930593"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..715
                     /locus_tag="VM910_10045"
     CDS             23..715
                     /locus_tag="VM910_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI62909.1"
                     /translation="MTGSHRRILVVEDDPETAGQLLEQLTSSGYQVDLASTGDEALSR
                     GATCDYAVITIDRMLPDIDGIAVMRQLRDDGIAAPFLIISALGEVDDRVRGLRAGGDD
                     YLVKPFSFLELLARLEALGRRSDTIAKETILRVGDLAIDLISRTASRRGKEILLLPRE
                     FQLLEYLARNEGHVVSRAMLLQHVWDLHFDPSTNIIDVYVGRLRRKVDDQQAYPLIHT
                     VRGVGYCLRAPG"
     gene            702..2081
                     /locus_tag="VM910_10050"
     CDS             702..2081
                     /locus_tag="VM910_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI62910.1"
                     /translation="MLLAKTLTSLTFKLALIAIATFGVIVSVIFTYVYLSTTAYVRSR
                     SDRAIVTESLSLRDAYEKSGRDALIDLIRQRTSDRSFAGHVYALADPSSTLLGGNLAK
                     WPSEPSRPSGWTEFRAAVPLPNAADPPLVRAMLETFPSGDRLLVGRDISDLDSFTAQI
                     KLAVISGVALIFLLAMMASILVTRRTVARIEQINATSRAIMQTGLDKRIPLRGTHDEW
                     DRVAENLNLMLERIETLMGEVKQVSDNVAHDLRTPLTRMRGRLEKAYHGARNSEADQL
                     LIGDTIADLDAVLRIFSSITRIAQIETQARKDAFRNVNLVEIACEVVELYDAAAEQDG
                     TRLSVTGAREAMVTGDRDLIFDAIANLVDNAIKHGRGGGKVVVAIENGDGGPLISIAD
                     DGPGIPPDEREHVFKRFYRLEQSRYTPGNGLGLSLVAAVARLHGARIEMLDNAPGLQF
                     NLRFAPPVG"
     gene            2239..2553
                     /locus_tag="VM910_10055"
     CDS             2239..2553
                     /locus_tag="VM910_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62911.1"
                     /translation="MPELPVADAVDFAELAAAPFGASPGNCWAVSGLADGDDAPVAVP
                     AAAPVDADTDPACALADDEVGTLRGSQPEAVEQTDCVEPAMPDEPVSGESNAAGGDAG
                     LT"
     gene            2792..4135
                     /locus_tag="VM910_10060"
     CDS             2792..4135
                     /locus_tag="VM910_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI62912.1"
                     /translation="MPAFAACPGIRTAIALVAAGLSLSSAASAQQLLPPLGKPAAAAP
                     RAPAAPSSRAASCHNGASFDKFLAELKQRAVSEGVSPNTIAAASPYLTYDQSIVNRDR
                     GQRVFGQLFTEFAGRMAAPYRMQNGQSHIRTYAAAFARAEKEYGVPRAVIAAFWGLES
                     DFGANMGSLPVLPSLVSLAYDCRRSERFQNETVAALRVIDRGDLEPDEMIGSWAGELG
                     QTQFLPTHYVTYAVDYDDDGRRNLLNSAPDVIGSTANYIATGLKWRRGEPWLEEVRVP
                     PDFPWDQADLTIQLPRSKFSALGVTYADGRPLRNDDLPASLLLPMGRTGPAFLAYANF
                     AAYTEWNNSLIYSTTAAYLATRIAGAPPMRRPAAPVAQLRFNEIKELQQLLVRSGYDV
                     GKVDGVLGQQSRTAVKAMQIKYGLPADSWPTLELLAKMRGTPRPLPAAAALPPMR"
     gene            4343..>4573
                     /locus_tag="VM910_10065"
     CDS             4343..>4573
                     /locus_tag="VM910_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HVI62913.1"
                     /translation="MSTPTKLFEPFKLGPLTLPNRLVMAPLTRNRAVPPGMVPSPLAI
                     EYYGQRASAGLLVTEASQVSQQGQGYQDTPGIY"
BASE COUNT          850 a         1469 c         1424 g          830 t
ORIGIN      
        1 tcgcgatcgt cggagcaagg tcatgacggg aagccaccgc cgaatactgg tcgtggagga
       61 cgacccggaa acggccggtc agctcttgga gcagctcacc agcagcggtt accaagtgga
      121 tctggcctcg accggcgacg aggcgttgag ccggggcgcg acttgcgact atgccgtcat
      181 cacgatcgat cgcatgctgc cggacatcga cggcatcgcg gtgatgcgtc aactgcgcga
      241 cgacggcatc gccgcgccgt ttttgatcat cagcgcgctt ggcgaggtcg acgacagggt
      301 gcgcggcttg cgtgccggcg gtgacgatta cctggtcaag cctttttcat tcctcgaact
      361 gctcgcccgt ctcgaagcgc tcggccggcg cagcgacacc atcgccaagg aaaccatcct
      421 gcgggtcggc gatctcgcga tcgacctgat ttcgagaacc gccagccgtc gcggcaagga
      481 gattctgctt ctgccacggg aattccagct tctggagtat ctcgcgcgaa acgaaggcca
      541 tgtcgtctcc agggcgatgt tgctgcagca tgtctgggat cttcacttcg atccgtcgac
      601 caacatcatc gacgtctatg tcggacgctt gcgtcgcaag gtcgacgacc agcaggccta
      661 tccgctgatc catacggtgc gcggtgtcgg gtactgtctc cgtgctcctg gctaagaccc
      721 tcacgtcatt gacgttcaag ctggcgctga tcgcgatcgc tacattcggc gtgatcgtgt
      781 cggtgatctt cacctacgtc tatctgtcga caaccgccta tgtgcgcagc cggtcggatc
      841 gtgccatcgt gaccgagtcc ttgagcctgc gggacgctta cgagaaatcg ggacgggatg
      901 cgttgatcga cctcatccgg cagcggacct cggacaggag ttttgcgggt cacgtctacg
      961 cgcttgccga tccgtcatcg acgttgctgg gcggcaacct cgcgaaatgg ccctccgagc
     1021 catcgcgccc atcgggatgg accgaatttc gcgcagccgt cccgctgccc aacgccgcgg
     1081 acccgccact cgtgcgggcc atgctcgaga catttcccag cggcgaccgt ttgctggtgg
     1141 gcagagacat cagcgacctc gacagcttta ccgcccagat caagctcgcc gtgatctccg
     1201 gcgtcgcgct gatattcctg ctagcgatga tggcaagcat cctggtgacg cgtcgaacgg
     1261 tggcgcgaat cgagcagatc aacgccacca gccgcgccat catgcaaacc ggtctcgaca
     1321 agcgtatccc gcttcggggc acccacgacg aatgggatcg cgtcgcagag aacctcaatc
     1381 tgatgctgga gcgcatcgag accctgatgg gggaagtcaa gcaggtcagc gacaacgtcg
     1441 cgcacgacct gcgaacgccg ctgacgcgga tgcgcggacg gctggagaag gcctatcacg
     1501 gtgcgcgcaa cagcgaggcc gatcaattgc tgatcggcga tacgatcgcc gatcttgacg
     1561 ctgtcctgcg gatattctcg tcgatcacgc ggatcgcgca gatcgagacg caagccagaa
     1621 aggacgcgtt ccgcaacgtc aacctcgtcg aaatcgcttg cgaggtggtc gagctgtacg
     1681 atgcggcagc cgaacaggat ggcacccgcc tgtccgtcac tggcgctcgt gaggcgatgg
     1741 tgacgggcga tcgcgatctg atcttcgatg cgatcgccaa tctcgtggac aatgcaatca
     1801 agcatggccg cggcggaggt aaagtcgtgg ttgcgatcga gaacggcgat ggcggtccgc
     1861 tcatctcgat cgccgatgac gggccaggga tcccgccgga cgagcgcgag catgttttca
     1921 agcgctttta tcgcctcgag caaagtcgct atacgcccgg aaacggtctc ggactgagcc
     1981 tggttgcggc cgttgcccgc ctgcatggcg cgcgcatcga aatgctcgat aacgcgccgg
     2041 gtcttcagtt caatctccgc tttgcgccac cggtgggcta gccgagacgg cgatgcagaa
     2101 acagaaatcg cggtattcct cggcgcgact gacgccgatc aacatccgcc gggcaagata
     2161 ccactgaaga cgccgagcga ctccccggtc gtcgtctgca ttgtcgggcc gcacagcggc
     2221 ggtgaggcgg cggcaggggt gccggagctg cccgtcgccg atgccgtcga ctttgccgaa
     2281 ttggccgcgg cgccgttcgg agcctcgccc ggcaattgct gggcggtttc gggattggcc
     2341 gacggggacg acgcgccggt ggccgttccc gccgctgctc ccgtagacgc agacaccgac
     2401 cctgcctgcg cattggcgga cgacgaggtc ggcacgctca ggggatcgca gccggaagcc
     2461 gttgaacaga cagattgcgt ggaaccggca atgcccgacg aacccgtatc gggcgaaagc
     2521 aatgcggccg gaggtgatgc aggactaacc tgagtattcg cctggccgct ggtctgtgcg
     2581 cgcgccattc cggtcacggc aagccaaaga accatcgcaa ggatcgggat tcgctccatc
     2641 gcgcgctcct gttctcaagc cgagcgcgtc gccctcgcaa agccaatgcg acggcaacga
     2701 ggcggttccc cacggaagat ttgcttttgg cccggtttcg gatatcccgg gaacctcaaa
     2761 tggcctgcgc acgaatcatc cggtgagatt gatgcccgcc tttgccgcct gccctggaat
     2821 tcgaactgcg atcgcactcg tcgccgcggg gctgtcgctc tcctcagcgg catcggccca
     2881 acagcttctt ccgcctctcg gcaagccggc cgccgccgcg ccgagagcac cggcggcacc
     2941 ctcctcccgc gcggcctcct gtcacaacgg cgcaagcttc gacaaattcc tcgccgagct
     3001 gaagcagcgc gcggtcagcg agggcgtttc cccgaacacg atcgcggcgg cttcgccata
     3061 cctcacttac gaccagagca tcgtcaaccg cgatcgcggc cagcgcgtgt tcggacagct
     3121 cttcacggaa ttcgcgggaa gaatggctgc gccctaccgg atgcagaacg gacagtcgca
     3181 catcagaacc tacgcggcgg cattcgcgcg cgcagagaag gaatacggcg tgccccgggc
     3241 cgtgatcgcc gcgttctggg gactggaaag cgatttcggc gccaatatgg gtagtcttcc
     3301 ggtactgccc tcgctggtat cgctcgccta tgactgccgg cgttcggagc gtttccagaa
     3361 cgagaccgtt gccgcgctga gggtgatcga tcgcggcgat ctcgagccag acgagatgat
     3421 cggctcctgg gccggcgaat tgggacagac gcaattcctg ccgacgcatt atgtcaccta
     3481 tgcggtcgac tatgatgacg acgggcgacg caatctgttg aacagcgcgc ccgatgtgat
     3541 cggctcgacc gcgaactaca ttgccaccgg cctcaaatgg cggcgcggcg agccatggct
     3601 ggaagaagta cgcgtgccgc cggattttcc ctgggaccag gcggatctga cgatccaact
     3661 gccgcgctcg aaattctccg cactcggtgt gacctatgcc gacggccggc cgctgcggaa
     3721 tgacgatctg ccggcttcgc tgctgttgcc gatggggaga acgggcccgg cgtttctggc
     3781 ctacgcgaat ttcgcggctt acaccgaatg gaacaattcg ctgatctatt cgaccaccgc
     3841 ggcctatctc gcaactcgca tcgcgggtgc tcccccgatg cgccggccgg ccgcgccggt
     3901 cgcgcaactg cggttcaacg agatcaagga attgcagcaa ttgctcgtgc gctccggcta
     3961 tgacgtcggc aaggtcgacg gtgtgctcgg tcagcaaagc cgtaccgccg tgaaggcgat
     4021 gcagatcaaa tatggcttgc cggcggattc ctggccgacc ctggaattat tggcgaagat
     4081 gcgcggcacc ccgcgcccgc tgcccgcggc ggctgccctt ccgccgatgc gataacatga
     4141 ccgtcaatgt cggaggacaa agcgcttcgc aaatccccgg caggcagttg atccgtttcg
     4201 cgcgattctg aaaatccgac cgaattcttc caaaatcatg cgccgcgcgg ctcgtctgcg
     4261 cgggccgtat ggctcgcggc attgcggaga tcgcggcaat gcacaattgt aggccatctt
     4321 tcatgtcatt cggaaaggcc atatgagcac gccgaccaaa ctattcgagc ccttcaagct
     4381 aggcccgctc acgcttccca atcgcctcgt catggcgccg ctgacgcgca atcgcgcggt
     4441 gccgccgggc atggtgccga gcccgctcgc aatcgaatat tacggccagc gtgcatccgc
     4501 cggacttctg gtcacggaag ccagccaggt ctcgcagcag ggccagggtt atcaggacac
     4561 gcccggcatc tac
//
LOCUS       DATLJT010000435         7555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_254848,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000435 DATLJT010000000
VERSION     DATLJT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254848"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1038
                     /locus_tag="VM910_10070"
     CDS             151..1038
                     /locus_tag="VM910_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62914.1"
                     /translation="MSASADDPDALAWISAFAQGLQERGWSVGSNVRIDYRWGAGDLD
                     RFRKYAAELVALAPDVILATAGSIVAALQQASRTVPIVFVTTIDPVGGGLVESLARPG
                     GNATGFSTREFSMMGKLLELLKELAPRLSRVAVIRDPAVPAGSGGFAAIQTVAPSFGV
                     ELTPIGVRTADEIERSITAFARSENGGVIVVGPTSSVQSHRNLIIALAARHRLPAVYS
                     GTVWTKAGGLISYGADPVDQYRRSAEYVDRILKGEKPADLPVQAPTKYELVINLKTAK
                     ALGLDVPTTVLARADEVIE"
     gene            1057..2046
                     /locus_tag="VM910_10075"
     CDS             1057..2046
                     /locus_tag="VM910_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI62915.1"
                     /translation="MKLPRRQFLHLAAGAAALAPLSRIAWAQTYPSKPVTIVVPFAAG
                     GPLDLRARSLAEHMRSALGQPIIIENVSGAAGSLGAGRVARSAPDGYTLAIGIWGTHV
                     VNGAVYRLQYDVLNDFEPVALLTNNSQLIVGKKTLPANDLKGLVAWLKANPHQALAGT
                     AGVGSPQHVFGILLQNATSTRVNFVHYRGGAPAMQDLVAGQIDLIVADLTTSLPQVRA
                     GSVKPYAFTGRSRSAAAPDVPTADEAGLPGFYTSVWSAIWAPKGTPKRVIGKLNAAIV
                     EALADPATRQRLVDLGDQVVPREEQAPERLRALQEAEIEKWWPIIKEAGIKAE"
     gene            2084..3064
                     /locus_tag="VM910_10080"
     CDS             2084..3064
                     /locus_tag="VM910_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI62916.1"
                     /translation="MKLPHRRQFLHLAAGAAALPAVSRVARAQAYPSRPVRIIVGFAA
                     GGPADTVARLIGQWLSERLGQPIVVDNRSGANGNIGTEIGVRAPPDGYTLLMTLAVNA
                     INAAVYDNLSFNFIRDTAPVASIASIPLVMEVNPSVPAKTVPEFIAYAKINPGKINMA
                     SSGVGTPLHVAGELFKMMAGVDMVHVPYRGEALALPDLISGQVQVLFGVMPASLGYIR
                     AGKLRALAVTTAKRQEPLPDVPTVGEFLPGFEARGWYGIVVPKATPTEIVEKLNKEIN
                     AALVDPNMKKRLTDLGAALFGGSPADFGKFIADETDKWRKVVTFAGIKAE"
     gene            3230..5581
                     /locus_tag="VM910_10085"
     CDS             3230..5581
                     /locus_tag="VM910_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI62917.1"
                     /translation="MSLKGAKSPTRDRKLRSTGTKARARVDRIRKPCADLEQQLEKYR
                     HELVEAREHLAEALEQQTATSEVLQVISSSPGQLEPVFQAILENATRICEAKFGMLYL
                     WEREGQYRVAALHGAPPRLAKERRRGTVIRSAPGSILGRVAQMKHTVHIADVRAEKNH
                     IDPPPAFTPSGIAIYGGARTALAVPMLKEGQLIGAFAIYRQEVRPFTDKQIELITSFA
                     NQAVIAIENTRLLSELRQRTDDLSESLEQQTATSEVLRVISSSPGELEPVFRAMLANA
                     VRICEAKFGTLYLREGDGFRAVAMHNAPAAYVEARATIVHPPPDTSLGQAAMTKRAAQ
                     VADVKQTKGYVEGNPFTVSAVDAGGYRTVLSVPMLKEGELIGAIAIHRQEVRPFTDKQ
                     IELVQNFANQAVIAIENTRLLNELRESLQQQTATADVLKVISRSTFDLQMVLNTLVES
                     AARLCEAEKAFIYRFDGELLRMAATYNISEPHKDFGKRNPIRPGRHTVAARAALERRT
                     VHIEDVHADPEYTYGAQDFAPHGSLLGVPMLRGGEELLGVIVLIMGEVQPFTEKQIEL
                     VTTFADQAVIAIENVRLFEEVQARTEELSESLQQQTATADVLKVISRSTFDLQTVLDT
                     LVESATRLCEADYAWLFQRDGDVFRLAAIYGHAADVHGRLKEYFQGREVPADRGSVTG
                     RAALEARVVHVPDVLADPDYTWSGAQEIGGYRSALGAPLLRKGDVAGVIFVAKKGAAA
                     VYRETDRAGDNLCRSGADRHREHPPAQRAARVAAAADRDRRRA"
     gene            5475..>7555
                     /locus_tag="VM910_10090"
     CDS             5475..>7555
                     /locus_tag="VM910_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI62918.1"
                     /translation="MTTFADQALIAIENTRLLNELRESLQQQTATADVLKVISGSPGE
                     LEPVFKAMLENAVRICEASFGNLLLYEGDVFRHVALHNAPKSWAADSERDPVPPRRSA
                     RILYRVADTRQVAHVADIAAENPDEPIAKIAGARTLLIVPMLKENDLIGAIAIYRQEV
                     RPFNDKQIDLVKNFAAQAVIAIENMRLLNELRESLQQQTATADVLKVISRSTFDLQAV
                     LDTLADSAVRLCDADQAVIRRRVGDAYPVAATHGLSQQQRDYLERNSPKPDRGSIFGR
                     ALCDGRTVHIPDIVADREFNRPGAATVLGLRAGVGVPLLREGIVVGVLVVGRIERRPF
                     SPKQIELLETFADQAVIAIENVRLFDEVQARTRELSESLEQQTATAEVLRVISNSPGE
                     LEPVFQTMLANAVRICEATFGNMYLRDGEVFRIAAAHNTPTPLLEHRRRVPLQRPTSA
                     FGRMVRTKEVVHVADVLADPAYAEREPEVVTGVELGGIRTLLIVPMLKEKDLMGALTI
                     YRQEVRPFNDKQIELVKNFANQAVIAIENTRLLTELRESLQQQTATADVLKVISRSTF
                     DLRSVLQTLVESAGRLCEADFAMITRQKDGVLFFAEAYGYSPEFIEYVRALPVERGRG
                     TATGRALLEGRVIHVADVLADPDYTWAEAQRLGGYRTVLGVPMLREGIAMGVLTLTRS
                     EVRPFTDKQIEL"
BASE COUNT         1451 a         2324 c         2390 g         1390 t
ORIGIN      
        1 gccttgacat accgccgacg ctgctcgccc gcgccgacga ggtgatcgaa tgaggcgacg
       61 acagttcatc acgctgctcg gcggcgcggc ggcgtggcgg gtcgcggcgc gcgacagcaa
      121 cgcgagcgcg tgcggcgcat cggcgtgctc atgagtgcga gcgcggacga tcccgatgcg
      181 ctcgcatgga tctctgcgtt tgcgcagggg ctgcaggaac gcggctggag cgtcggcagc
      241 aatgtgcgca tcgactatcg ctggggtgcg ggcgatctcg atcgctttcg caaatacgcg
      301 gcggaattgg tggcgctcgc gccggacgtc atccttgcga ccgcgggctc gatcgtggcc
      361 gctctgcaac aggcgagccg caccgtaccg atcgtgttcg tgactacgat tgatccggtg
      421 ggtggcggct tggtggaaag tctggcgcgg ccgggcggca acgccacagg tttttccacg
      481 cgcgaattca gcatgatggg gaaattgcta gaactcctta aagagctcgc gccgcgcctg
      541 tcgcgggtcg ccgtgatccg ggatcccgcg gtaccggccg ggtcaggagg atttgccgcg
      601 atccaaaccg tcgcgccttc atttggcgtg gagctaaccc ccatcggcgt gcgcaccgcc
      661 gatgagatcg agcgcagcat tacggcgttc gcgcggtccg agaacggcgg ggttatcgtg
      721 gtcgggccca catcgtccgt gcagtctcat cgcaatctca tcatcgcgct cgcggcccgc
      781 caccggctgc ccgcagtcta ctccggcacg gtgtggacca aggccggcgg gctgatctcc
      841 tatggcgccg atccagttga ccaataccgg cgctccgccg aatacgtcga tcgcatcctc
      901 aagggcgaga agccggctga cttgccggtg caggcgccga ccaagtacga actggtcatc
      961 aatctgaaga cagccaaggc cctcggcctc gatgtgccca cgacagtgct cgcccgcgcc
     1021 gacgaggtga tcgaatgatg tgaagggagg ttcaaaatga aacttccccg ccgacaattt
     1081 ctgcatctgg ccgccggcgc tgccgcgctc gcaccgctgt cgcgcatcgc gtgggcccag
     1141 acctatccct cgaaaccagt tacgatcgtg gtgccattcg ccgcaggcgg tccattggat
     1201 ttgcgggcac gctctttggc cgagcatatg cggtcggcgc tcggtcagcc catcatcatt
     1261 gaaaacgtta gcggcgctgc cggcagtctt ggcgccggcc gggtcgctcg ctctgcaccc
     1321 gacggttata cgcttgcgat cggcatctgg ggcacgcacg tcgtgaacgg agctgtctac
     1381 cggctgcaat acgacgtgct gaatgatttc gagccggtcg ctcttctaac aaacaattcg
     1441 cagttgatag ttggaaagaa gaccttgcct gcgaatgatc taaaaggtct cgtcgcttgg
     1501 ttgaaggcta acccgcatca agcgttggcg ggcacggcgg gcgtaggtag cccgcagcat
     1561 gtcttcggca ttcttttgca gaacgctacc agcacccgcg ttaactttgt gcattaccgc
     1621 ggcggcgccc cggcaatgca agacttggtg gccggtcaga tcgacttgat tgttgccgat
     1681 ctcaccacct cgctgccgca agtgcgtgcg ggcagcgtca aaccctatgc gttcaccggc
     1741 aggagccgct cggcagcggc gcctgacgtt ccgacggcgg acgaggctgg attacccggg
     1801 ttctacactt cggtctggag tgcgatttgg gctccaaagg gcacgccgaa gcgcgtcatc
     1861 ggcaagctca acgcggcgat tgtcgaggcg ctggcggatc cagcgacccg tcagcggctc
     1921 gtggacctgg gtgaccaggt tgtgccgcgc gaggagcaag cgcccgagag actgcgagcc
     1981 ctccaggagg ccgagatcga aaaatggtgg ccgatcatca aagaggccgg catcaaggcg
     2041 gaatgaagcg tcgcgccgcc ggcttttcag ggaggaggcg cctatgaaac ttccccaccg
     2101 aagacaattt ctccatctgg ccgccggcgc tgccgcgctg ccggccgtgt cgcgtgtcgc
     2161 gcgggcgcaa gcctatccat cgcggccggt gcgcatcatt gtcggctttg ccgctggcgg
     2221 gccggccgac accgtcgcac gcctgatcgg tcaatggctg tcggagcggc tcggccagcc
     2281 gatcgtcgtc gacaaccggt ctggcgccaa cggcaatatc gggacggaaa tcggcgtgag
     2341 agcgccgccg gacggctaca cgctcctcat gacccttgcg gtgaacgcaa tcaacgcggc
     2401 ggtctacgac aacctctctt tcaatttcat ccgcgacacg gcgccggtgg cgagcattgc
     2461 cagtatcccc ctcgtcatgg aagtgaatcc atcggttccg gcgaagacgg ttcccgagtt
     2521 catcgcctat gccaagatca atccaggcaa gatcaacatg gcttcgagcg gcgtcgggac
     2581 cccgctccat gtcgccgggg agctgttcaa gatgatggcc ggcgtcgaca tggtccacgt
     2641 tccttaccgc ggcgaggcgc tcgcactgcc tgatctgatc agtgggcagg tgcaagtcct
     2701 attcggcgtc atgcctgcgt cgcttgggta tatcagggcc ggcaagctgc gcgcgctggc
     2761 agtgaccacc gcaaagcgtc aagagccttt gccggacgtc ccgaccgtcg gcgaatttct
     2821 gcccggcttc gaggcgagag gctggtatgg catcgtcgtg cccaaagcca cgcccacaga
     2881 gattgtcgag aagcttaaca aggaaatcaa tgccgctctc gtcgacccca acatgaagaa
     2941 gcgcctcacc gacctgggag ctgcgctgtt cggcggttcg cccgccgact ttggaaagtt
     3001 catagccgac gaaaccgaca agtggcgcaa ggtggtcacg tttgcgggca tcaaggcgga
     3061 ataatcacct gcccgacatt ccataactgc cgttcggcga atagcggcga ggaggtgatc
     3121 gagtgcgaaa aggcgcaagt tcatcgtgcg gttctgttag gattgacagg ctccatcctt
     3181 ggctgggccg accggaaggg gggccagtgg ctgtgccgac gaggtgacct tgtctctcaa
     3241 aggcgcaaag agcccaacgc gtgaccgcaa gcttcgttcc accggaacga aggcgagagc
     3301 gcgcgttgat cggatacgta agccgtgtgc cgatctggaa cagcagctcg aaaaatacag
     3361 gcacgaactt gtcgaggctc gggagcacct tgccgaggca ctggagcagc agaccgcgac
     3421 ctcggaggtc ctgcaggtca tcagcagttc gcccggccaa ctggagccgg tgttccaggc
     3481 cattctggag aacgcaaccc gaatctgcga ggccaagttc ggcatgctgt acctgtggga
     3541 gcgggaagga caataccgcg tcgccgcact gcacggtgca ccgcctcggc tggcaaagga
     3601 acgccgccga ggaactgtta tccgttctgc gcctgggtcc attctcgggc gggtcgcgca
     3661 gatgaagcac acggtccaca ttgccgatgt tcgcgccgaa aagaatcaca tcgatccccc
     3721 gccagccttc actccgtcgg ggatcgccat ttacggcgga gcgcggaccg cactggctgt
     3781 gccgatgctt aaggagggac agctgatcgg tgcatttgcc atctaccgtc aggaggttcg
     3841 gcctttcacc gacaagcaaa tcgaactgat cacgagcttc gccaaccagg ccgtcatcgc
     3901 catcgagaac acgcgcctgc tcagtgagct gcgccagcgc accgacgatc tcagcgaatc
     3961 gctggagcaa cagacagcga cttcggaagt gctgcgtgtc atttcaagct ctccgggcga
     4021 gctggaaccc gtattccggg ccatgttggc gaacgcggtg cgcatctgcg aggccaagtt
     4081 tggaacgctt tatctgcgcg aaggtgatgg tttccgcgcg gtagcgatgc acaacgcacc
     4141 ggcggcctat gtcgaggcac gcgcgacgat cgttcatccg ccgccagata ccagcctcgg
     4201 gcaggcggca atgaccaagc gggccgccca ggttgcggac gtgaaacaga cgaagggata
     4261 cgtggaaggc aatcccttca ccgtctccgc ggtcgacgct gggggttatc gcaccgtgct
     4321 tagcgtcccg atgcttaagg aaggcgagct gatcggcgcg atcgccattc accgccagga
     4381 ggtgcgcccg ttcaccgaca agcagatcga attggtccag aatttcgcca atcaggccgt
     4441 tatcgcgatc gagaacaccc ggctgctcaa cgagctgcgc gaatccctcc aacagcagac
     4501 cgccacagcc gatgtgctca aggtcatcag ccgctcgacc ttcgatctgc agatggtact
     4561 caacacgctg gtggagtcgg cggccaggct gtgtgaggcg gaaaaggcat ttatctaccg
     4621 gttcgacggc gaacttctcc gcatggcggc cacctacaac atatcagaac cgcacaaaga
     4681 tttcggtaag cgtaacccga ttcgacccgg acggcacacg gttgctgcgc gcgccgcgct
     4741 tgaacgccgg acggtccata ttgaggatgt gcacgctgat cctgaatata cgtacggagc
     4801 gcaagacttc gcaccccatg ggtcacttct gggagttccg atgctcaggg gcggcgagga
     4861 gctcctcggg gtcatcgttc tgatcatggg tgaagtccag cccttcaccg aaaagcagat
     4921 cgagctggtc accactttcg ccgaccaggc ggtgattgcg atcgagaacg tgcggctgtt
     4981 cgaggaggta caggcgcgca ctgaggaact gagcgagtcg ctccagcaac aaaccgccac
     5041 cgctgatgtg ctcaaggtca tcagccgctc gacattcgac ctgcagacgg tgcttgacac
     5101 cctggtcgag tcggcgactc gcctgtgcga ggcggattac gcctggctgt tccagcgcga
     5161 cggtgatgtt ttccgcctgg ccgcaatcta cggccatgcc gccgacgtgc acggacgcct
     5221 gaaggaatat ttccaagggc gcgaggtgcc ggcggaccga ggcagcgtca ccggacgggc
     5281 cgccctagag gccagggtgg ttcacgtccc cgacgtgcta gcggacccgg attacacgtg
     5341 gagcggagcg caggagatcg gcggctatcg ctccgcactc ggtgccccgc tgttgcgcaa
     5401 gggggatgtg gcgggggtga tcttcgtagc caaaaaaggt gccgcagccg tttaccgcga
     5461 aacagatcga gctggtgaca acctttgccg atcaggcgct gatcgccatc gagaacaccc
     5521 gcctgctcaa cgagctgcgc gagtcgctgc agcagcagac cgcgaccgcc gacgtgctta
     5581 aggtcatcag cggctcgccc ggcgaactgg agccagtgtt caaggctatg cttgagaacg
     5641 ccgttcgcat ctgcgaggca agcttcggca acctgttgct ctacgaaggc gatgtattca
     5701 ggcacgttgc gctgcacaat gcaccgaagt cttgggccgc tgatagcgag cgcgatccgg
     5761 ttcctcctcg tcgctctgcc cgtatcctct accgtgtcgc tgacacgagg caggtcgcac
     5821 acgtcgccga catcgctgcg gaaaatcccg atgagccgat tgccaagatt gccggagccc
     5881 ggacgcttct gattgtaccg atgctcaagg agaacgacct gattggtgcg atcgctatct
     5941 atcgccagga ggtccgcccc ttcaacgata agcagataga tttggtcaag aatttcgccg
     6001 cgcaggccgt catcgcgatc gagaacatgc gtctcctcaa tgagcttcgc gaatcgctgc
     6061 agcagcagac tgccaccgcc gacgtgctca aggtcatcag ccgctcgacc ttcgatttgc
     6121 aggcagtgct cgatacattg gcagattcgg cagtccggct ttgcgatgcg gatcaagccg
     6181 ttattcgccg ccgggtcggc gacgcctacc ctgtagccgc aacgcacggc ctctcgcagc
     6241 aacagcgcga ctatctcgag cgcaactctc cgaagccgga tcgcggatcg attttcgggc
     6301 gcgcgctttg cgatggtcgc accgtccaca tcccggacat cgtcgccgat cgggaattca
     6361 atcgtcccgg ggcggccacg gttttaggcc tgcgtgctgg cgtcggcgtg ccgctgctgc
     6421 gcgagggaat cgtcgtcgga gtcctcgtgg tgggccgcat cgaacggcgg cccttcagtc
     6481 cgaagcagat cgagctgctt gagaccttcg ccgatcaagc agtgatcgcg atcgagaacg
     6541 tgcggctgtt tgacgaggtg caggcgcgca cgcgcgaact aagcgagtcg ttggagcagc
     6601 agacggctac tgcggaggtg ttgcgggtca tttcgaattc gccgggcgag ctggagccgg
     6661 tatttcagac catgctcgcc aacgcggtgc gcatttgcga ggctacgttc ggcaacatgt
     6721 atctccgcga cggagaggtg tttcgtatcg ctgcggcgca caacacgccg accccgctcc
     6781 tcgagcatcg gcggcgcgta cccctgcaac gtccgacatc cgcctttggg cgcatggtga
     6841 ggaccaagga ggtagtccat gtcgccgacg tcttggcaga cccagcgtac gccgagcgcg
     6901 aaccagaggt tgtcaccggc gtcgagctcg gtggcatacg gacacttctc atcgtgccta
     6961 tgctcaagga aaaggacctg atgggggcgc tcacgattta ccggcaagag gtccgtccct
     7021 tcaacgacaa gcagatcgag ctagttaaga attttgccaa ccaggccgtc atcgccatcg
     7081 agaatacgcg tcttctcacc gagctgcgcg aatcgctaca gcagcagact gcgaccgccg
     7141 acgtcctcaa ggtcatcagc cgctcgacgt tcgacctcag gtcggtgctg caaacgctcg
     7201 ttgaatcggc cggccgcctg tgtgaggccg acttcgccat gataactcgg cagaaagacg
     7261 gggtgctctt cttcgcagag gcttacgggt attcgcccga gttcatcgaa tacgtcaggg
     7321 ctttacccgt cgagcgggga cgcgggacgg cgactggacg ggccttgtta gaaggtcggg
     7381 tgatccatgt tgccgatgtc ttggccgatc cagactacac ctgggcggag gcccagagat
     7441 tgggcggcta ccgcacggtc ctcggcgtgc cgatgttgcg cgagggcatt gcgatgggtg
     7501 ttttgacgtt gacgcggtcc gaggtaaggc cattcaccga caagcagatc gaact
//
LOCUS       DATLJT010000436         4627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2092762,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000436 DATLJT010000000
VERSION     DATLJT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092762"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VM910_10095"
     CDS             <1..521
                     /locus_tag="VM910_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HVI62919.1"
                     /translation="IVQKYLHDLAGWNALPTEAQELIIGRSKLSDIEIDESIRPSSAH
                     NSLNTIVEDGKEIKIVRDNMPFGQVGQGEFGTYFIGYSRSPRTMEQMLENMFVGRPPG
                     NYDRILDFSRAVTGNLFFVPSATFLDEVTPDAPSPAERAAVSPAVESSQPIPPVPDGS
                     LGIGSLKGKARS"
     gene            518..1327
                     /locus_tag="VM910_10100"
     CDS             518..1327
                     /locus_tag="VM910_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HVI62920.1"
                     /translation="MNNLHRELAPISDAAWAQIQEETSRTLKRYLAARRVVDLHGPSG
                     VALSAVGTGHLQTIAAPGDGIIARQREVKALVELRVPFELDRQMIDDVERGANDSDWQ
                     PAKDAAKTIAFAEDGAIFEGYSAGGIRGIRQGTSNPIEPLPTDVRNYPDAIAHSLSVL
                     RLVGVNGPYSVLLGAEAYTAVAEARDYGYPVLEHLRRIIDGEIIWAPAINGAFVLTTR
                     GGDFDLHIGQDFSIGYSSHTDTVVRLYLQETFTFLLLTAEAAVALSPATPT"
     gene            2724..3434
                     /locus_tag="VM910_10105"
     CDS             2724..3434
                     /locus_tag="VM910_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI62921.1"
                     /translation="MTSQSAFAEVAALAGDPARAGMLHALMDGRALTASELARVAGIT
                     PQTASGHLARLAVAGLLAVEKQGRHRYHRLASPAVAQMMESIMQVASGLDTARPRLVV
                     GPRDAALRAARTCYDHLAGRLGVALADALVEGGRVELASDGGMVTDAGVAFFGRVGID
                     LDRVSARSSGRSARVLCRPCLDWSERRLHLAGTIGAALCAHCFAKGWIHRNNCTRAVT
                     ITPKGRHVFRDEFDVRLE"
     gene            3551..3841
                     /locus_tag="VM910_10110"
     CDS             3551..3841
                     /locus_tag="VM910_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI62922.1"
                     /translation="MTALSDPTRRLVFERILGAPRPVAAIAEGLPVSRPAVSQHLKVL
                     KEAGLITERRNGTRRLYAANPQALGELRAAIEAMWHEALGSMAVALDEEREG"
     gene            3838..4305
                     /locus_tag="VM910_10115"
     CDS             3838..4305
                     /locus_tag="VM910_10115"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI62923.1"
                     /translation="MTIARFPVPPIIKTVTVRAAPARAFALFADDFARWWPLARTHTG
                     PDPVDCAIEPRVGGRVFERAADGRETPWGTVLAYDPPHRLAFSWIVELPAGMEQLVEI
                     RFTAEDRGTRVELTHSGWEQLGDAAASLRERYDRGWGSVFERYFVTYANSVAT"
BASE COUNT          912 a         1449 c         1482 g          784 t
ORIGIN      
        1 tgatcgtgca gaagtatctc cacgatctcg ccggatggaa tgcgctgccc accgaggcgc
       61 aggaactcat cattggccgc agcaaactgt ccgacatcga gatcgacgaa tcgatcaggc
      121 cgagctcggc ccacaattcg ctgaacacga tcgtagagga cggcaaggag ataaagatcg
      181 tccgcgacaa catgccgttc ggtcaggtgg ggcagggcga gttcggcacc tactttatcg
      241 gctacagccg ttcgccgcgc accatggaac aaatgctgga aaacatgttc gtcggccgcc
      301 cgccgggcaa ttatgaccgc attctcgact tcagccgagc cgtcaccgga aacctcttct
      361 ttgtgccgtc cgcgacgttt ctcgacgagg tgacgcccga cgcgccatcg ccggcagagc
      421 gtgccgccgt atcgccggcg gtcgagtcct cgcaacccat cccgccggtt cccgatggtt
      481 ccctcggcat cggctcgctc aaaggaaaag cccgatcatg aacaatctgc accgcgagct
      541 tgctccgatc tccgatgcgg cgtgggcgca gatccaggag gagacgtcgc gcaccctcaa
      601 acgctatctc gctgctcgcc gcgtcgtcga cctgcacggc ccgagcggtg tcgcgctgtc
      661 tgccgtcggc accggccatt tgcagacgat cgcagcgccc ggcgacggca tcatcgcacg
      721 gcagcgcgag gtcaaggcgc tggtcgaatt gcgtgtgccg tttgagctcg atcgccagat
      781 gatcgacgac gtcgagcgcg gggccaacga ctcggactgg caaccggcga aggatgccgc
      841 gaagactatt gcctttgccg aggatggcgc gatcttcgag gggtattcgg cgggaggcat
      901 tcgagggatc cgtcagggga ccagcaaccc gatcgagcca ctgcccaccg atgtgcgcaa
      961 ctaccccgac gccatcgccc actccttgag cgtattgcgc ctggtcggcg tcaatggccc
     1021 gtattcggtg ctgctgggtg ccgaagccta taccgccgtg gcagaagcca gagactacgg
     1081 gtatccagtg ctggagcacc tcagacgtat catcgacggc gagatcatct gggccccggc
     1141 catcaacggc gcgttcgtgc tgaccacccg cggcggcgat ttcgatctgc acatcggcca
     1201 ggatttctcg atcggctact cgagccacac cgacaccgtg gtgcgtctct acttgcagga
     1261 aactttcacc ttcttactgc tcacggccga ggccgccgtg gccctgtcgc ccgctacgcc
     1321 gacttagaaa ccgtgcgtag tcggtttcga cccagcaaaa atgcgccaat tgccagtcct
     1381 tgagcaaacg cggcagcgac aaacaggccc ggagccgcgg acgaggctgt gcctgttgag
     1441 tgcggggcga gttccctgac cagaccaaag gtcgcgggcg cgaaggcata ggcggcttgc
     1501 gcgacggcaa cggccaggac aaccactcgc tgcacgtggt tttcgcggaa ttcgagttga
     1561 gcaatgagcg gcgcaaggac gtcgcattgc caattctgac gccgaacagg acaaccccga
     1621 gaaagagcag cggaacactc ggcccgcggc gagaatgaac atgatcgagc cggtaatttg
     1681 cacggcatag ctggccgaag ccacaacccg tttatccgca tcggtgggca tcacccagcc
     1741 cacgagtgtg cgacccgcga ttgccgaagt ggtggccagc cccatcgtca aggcggcaca
     1801 ttgtgcgccg agggccggca ccagcagcga aaatagatgc gtgatcaaac cgatctgcgg
     1861 ggacaatccc aacgccatcc cggcagaagc gtcaaaaatt tcagatcgtg ccaaagcgac
     1921 gctcccggcc gcggctgcgc cgccggtgaa cggaccaacg ctacaggtgt gagccgagca
     1981 tcaccgtccg gccgcaatcc catctctcga ggagtgcgcg aaaagagccg atcggcgaga
     2041 acccacattg taaccgccat gacaaccccg attgttgctg ctgcgagcgg gaagcccagt
     2101 acgctgatgc cagccaccca gagcggcgaa aagacgaccc ctccgatgct cgccccattg
     2161 tatgccatgg atagtgcagc gaggcgggtg cgcacaaacc acggagagac gatagcgttg
     2221 actggagcgg cgcccatcgc cgcccagccc gcgtcgctga agagtgtgga gacaaagagt
     2281 tgccagggtg cggtggcact ggaccagccg aggatgccaa cggctaagga gattgcaccc
     2341 gccttcgtcg ccaccggaac gccgaaacgc atatagaatg ttggcagatt ggccacgacg
     2401 accgcaccaa tgacgaaatg tgtagtcaac ggctgtggag atcagtgcca ccggccagcc
     2461 cctattctcc tgaatagctt gcaagtagac cggtggggcg taaaagctga tcccccaacc
     2521 caagaccgcc aagacgaagg cagcaccaac gacacgccaa ccaaagaaga tgccggtgcg
     2581 cctcgtcatg ctcgtcgcaa ccgcgttttg cgtcccttgc attccaagag cctgcacggc
     2641 ggcggtggaa aatgttacgg aaacgcccga agcatcggga cgccgaaagg cgtagattgc
     2701 ggtcgcccaa caaggaaggg acgatgacga gccagtccgc attcgctgaa gtcgccgctc
     2761 tggcgggaga tccagcccgt gccggaatgc tgcatgcgct gatggacgga cgtgcactta
     2821 cggcgtcaga gttggctcgc gtggctggca tcacgccgca gaccgcgagc ggtcatctgg
     2881 cgcgtttggc ggtggctggc cttctcgctg tcgagaagca aggccggcat cgctaccacc
     2941 ggcttgcatc accggcggtg gcccagatga tggaaagcat catgcaggtg gcgtccggcc
     3001 tggacacggc ccgaccgcgg ctcgttgtcg gtccgcgaga cgcagcgttg cgggcggcac
     3061 ggacctgcta cgaccatctt gccggccgtc tcggcgtggc gctcgcagac gcgttggtcg
     3121 agggcggccg cgtggagctg gcgagcgacg gaggaatggt taccgacgca ggcgtcgcgt
     3181 tttttgggag ggtcggcatc gacctcgata gggtttcagc gcgtagcagc ggacgctcag
     3241 ctcgcgttct gtgcagacca tgtctcgact ggagcgaacg acgccttcac ctggccggca
     3301 cgatcggcgc agccctatgc gcgcactgct tcgcgaaggg ctggatacac cgcaacaact
     3361 gcacccgcgc cgtcacgatc acaccgaagg gccgacacgt ttttcgcgac gagttcgatg
     3421 tgaggttgga gtagtttctt cggcacttcg tgtcggcggc catccgcgtc ggctgcggtg
     3481 gagttagcga tgcgcgcttg acagcgacgg taagcctcta ctaacgtaag tcatggctta
     3541 cgaagcggcc ttgaccgcgc tttccgatcc gacgcgacgg ctggttttcg agcgcattct
     3601 cggcgcgccg cggcccgtcg ccgccattgc cgaaggtttg ccggtcagcc gtcccgccgt
     3661 ctcgcaacac ctgaaggtgt tgaaggaggc tggactgatc accgagcggc gcaatggcac
     3721 acggcggctc tatgcggcga acccgcaggc gctcggtgag ctgcgcgcgg ccatcgaagc
     3781 gatgtggcac gaggcgctcg gcagcatggc tgtagcgctc gacgaggagc gggagggatg
     3841 acgattgccc gctttccggt tccgccgatc ataaagacgg tgacggtacg cgcggcgccg
     3901 gcgcgggcgt tcgccctgtt tgcagacgat ttcgcgcgct ggtggccgct ggcgcggacc
     3961 cacaccggcc ccgatccggt ggactgcgcg atcgagccgc gggtcggtgg gcgggtcttc
     4021 gaacgggcgg ccgatgggcg cgaaacgcca tggggaactg tgctcgcgta cgacccgccg
     4081 catcgtctcg ccttttcctg gattgtcgaa ctgccggccg ggatggaaca actggtcgag
     4141 atccgcttta ccgcagagga ccgcggcacc agggtcgagc tgacgcattc gggctgggaa
     4201 caactcggtg acgccgccgc aagcctgcgc gagcggtatg accgcggctg gggctccgtg
     4261 ttcgagcgct atttcgtcac ctacgcgaat tccgttgcaa cctgaactgc ggaggtaaca
     4321 tgaagtgacg catagtcgtt tacgcccgcg accagctgat cggaccagcg atgggcaccg
     4381 cggctgaagc ttgggcggtt cgtagaagcc ttaaacgcgg tggtatggtg gctgtgcgac
     4441 cgctttcatc tgctcgcctc cgatgtgcgc ggccacccag acagtagccg gaaaatccct
     4501 tgatcaagga gagaacgatg caactgtacc atttcccgtc acctaatccg cagaaggtca
     4561 ctttcgctct caaggagctg gggctcgatt gcgagatcgt gccggtcgat ctggggaagg
     4621 gggaaca
//
LOCUS       DATLJT010000437         2791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4390112,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000437 DATLJT010000000
VERSION     DATLJT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390112"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VM910_10120"
     CDS             complement(<1..284)
                     /locus_tag="VM910_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62924.1"
                     /translation="MTVNRHNLLVLPVAATGLLLLGAPLALAATAYIRTDLVSNHLTL
                     VQPPDPNLRNAWGVANSPGGPLWVSDNNAGVATVYDGNGVKINLTVTIPLP"
     gene            complement(418..1314)
                     /locus_tag="VM910_10125"
     CDS             complement(418..1314)
                     /locus_tag="VM910_10125"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI62925.1"
                     /translation="MRGPNREATQPRRRQLRLTEVWQGLFGLTISQGAIANVLARVGE
                     AIAAPAERIAAEVRASEVIASDETSARVKGKTWWQWTFGCATAVYHVIAPTRGKCVPT
                     DFLGGMKPQMWLSDRLAAQLGHAEEHQFCLAHLIRDAQYAIDHGDTIFAPDFKDLLKD
                     ACAVGRRRPDLADATIAAHRRRLERQLERLLARKPTDPEGRKLRDAVYVDCSDKLFVF
                     LKRRDAEPTNNESERALRPSVIFRKVTNGFRSEWGAKNYAALCSIVETGRRNGRSALT
                     AIRNALAQSAAVAAGARPAHAG"
     gene            1791..2270
                     /locus_tag="VM910_10130"
     CDS             1791..2270
                     /locus_tag="VM910_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62926.1"
                     /translation="MPQLQLPIFPDGVTYITNHLAFRRQDGQVTYFNGHMPVFSHAET
                     DIATFRMITSQFCVTGHVKQSDIIRAFGVASISVKRSVKIYREKGPKGFYAPRVTRGP
                     AVLVEDVVSEIEGRLASGTTPADVAKTLGLKLNTMQKAMREGRIRAPVKKNSLAMPR"
     gene            2420..>2791
                     /locus_tag="VM910_10135"
     CDS             2420..>2791
                     /locus_tag="VM910_10135"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62927.1"
                     /translation="MGRGATATLERLAASLGQLEEAAPRFACALDIPKGGVLLALPAL
                     LVSGLLRHSAKYFHLPAGFYGLTSIFLLLAFMALGRLRSIEALRYYAPGEWGKLLGLD
                     RAPEVRTLRIKLKHLADQEQAF"
BASE COUNT          504 a          848 c          915 g          524 t
ORIGIN      
        1 ggcagcggga tcgtcaccgt caaattgatc ttcacgccgt tgccgtcgta gaccgtggcg
       61 acaccggcgt tgttgtcgga aacccatagc gggccgccgg gcgagttcgc gaccccccac
      121 gcgttccgca gattcggatc cggcggttgg acgagggtca gatggttcga gacgaggtcg
      181 gtgcggatat aggccgtagc tgcaagcgcc agcggggcac cgagcaggag gagcccggtt
      241 gctgcgacag gtaggacaag taagttatgt ctgttgacag tcatcatatc ctcccgttgg
      301 attgaaacaa gtgtgtgtag cgggcgttat cttgagaacc cgccaaattg ccatggcatc
      361 caaccagtgc tcagaacctc atcgttattg tcaaagcagt ccctattccg taactactca
      421 ccccgcgtgc gccggtcgcg cgccggctgc cacggccgcg gattgtgcca gggcgttgcg
      481 gatggcggtg agagcgctgc ggccgttgcg acggccggtt tcgacaattg agcagagggc
      541 ggcgtagttt ttcgcgcccc actcggagcg gaacccgttc gtcactttgc ggaagatcac
      601 cgacggcctc agtgcccgct cgctttcgtt gttggtcggc tcggcgtcgc ggcgcttcag
      661 gaacacgaac agcttgtcgc tgcagtcgac atacaccgcg tctcgcagct tgcggccctc
      721 gggatcggtc ggcttgcgag cgagcagccg ctccagttgc ctttccagac ggcggcgatg
      781 cgcggcaatg gtggcgtcgg cgagatcggg ccggcggcgg ccgaccgcac atgcgtcctt
      841 gagcagatcc ttgaagtcag gcgcgaagat cgtatcgccg tggtcgatgg catactgtgc
      901 atcgcggatc agatgcgcga ggcagaactg atgctcctcg gcgtgcccca actgcgcggc
      961 cagcctgtcc gacagccaca tctggggctt catgccaccg agaaagtctg tcggcacgca
     1021 cttgccgcgc gtcggtgcga tcacatgata gacggcggtg gcgcagccga acgtccactg
     1081 ccaccaggtc ttacccttca cgcgcgcact cgtctcgtcg ctcgcgatca cctcgcttgc
     1141 gcgcacctct gccgcgatcc gttcggccgg cgctgcgatt gcctcgccga cgcgggcgag
     1201 cacgttggcg atggcgccct gtgagatcgt gaggccgaag aggccctgcc agacctcggt
     1261 gagccggagc tgacggcggc gtggctgcgt ggcttctctg ttggggcctc taattcgacc
     1321 gagaactcag cggttggaaa ccgaacacca gccgcgcacc taccgatgct cgctttcatc
     1381 gccatcgcgg ccaacatctg gccaaaatga ggaaagggtt cagcacccga ccggaccgga
     1441 tacgaagctc cggcgccgcg gcttgggcgt cagcctcgcg ctgtaacggg atcggctgca
     1501 attgcgcaat cgcagtggtc agggggcggc gcgacgaggg ttgatacatc acgactgcgt
     1561 ggctgggcat gtcggatacg aacttgcaaa gtgaaaattc agacctcctg agctctggcg
     1621 gctcggtgga aggtaagcta gggccggcaa atcgctcgtc gcatcagaat cttgcacaag
     1681 aaaggtcggc acatgggggg cagcacttgg ccgaatcgct gggattctga gaatgaccgg
     1741 tccgcggcgg atacgcggtg ttttgccgct cgtgccgcgc gaggtcggcc atgccccaac
     1801 tgcagctacc cattttcccc gatggggtca cgtacatcac gaaccacttg gccttcagga
     1861 ggcaggacgg tcaggtcacc tatttcaacg gccacatgcc cgtgtttagc catgccgaga
     1921 ccgacatcgc cacctttcgc atgatcacca gccagttctg cgtcaccggc catgtcaagc
     1981 agagcgatat catccgcgcc tttggggtgg cgtcgattag tgtcaagcgg tcggtgaaga
     2041 tctaccgcga gaagggtccg aagggattct acgctccgcg cgtgacgcgc ggtccggcgg
     2101 tgctggtgga ggatgtggtg agtgagatcg aggggcgtct tgcgagcggc accactccgg
     2161 cagatgtcgc caagacgctg ggactgaagc tcaacaccat gcagaaggcg atgcgcgagg
     2221 gaaggatccg cgcgccggtt aaaaaaaatt ccttggcgat gccgcgctaa ccgcgaacag
     2281 cccaagcgag agtgccgctg cggtttcttc cggttcggcg gaggagagga gcgcaacgag
     2341 tgcgcagcct tcgctgaacg gcgcacaacc gttacccagc accaagagcg agcgggcaga
     2401 agccgattgt gccgcgccga tggggcgcgg cgcgaccgct acgctggagc ggctggcggc
     2461 gagccttggg caactggagg aggccgctcc gcgctttgcc tgcgctctcg acattccgaa
     2521 aggcggcgtc ctgctggctc tgccggcgct cttggtcagc gggctcttgc ggcattctgc
     2581 caagtacttt cacctgccgg ctggctttta cggtctcaca agcatctttc tgcttctggc
     2641 cttcatggca ctgggccggc tcaggtcgat cgaggcactg cgttattacg cgccgggaga
     2701 atggggcaaa ttgctgggcc tcgaccgcgc tccagaagtg cgcaccttgc gcatcaagct
     2761 caagcatttg gctgaccagg aacaggcgtt t
//
LOCUS       DATLJT010000438         4066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1862815,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000438 DATLJT010000000
VERSION     DATLJT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862815"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VM910_10140"
     CDS             complement(<1..807)
                     /locus_tag="VM910_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVI62928.1"
                     /translation="MELPTSGNETQSTTEAPNDGKERNTRAATRVPSIIVGTVAAIVV
                     ALSIFYLLRPEPLLVQGEADATRFDIAARVDGRVKEIPVERGQNVPAGAVLVRIDNPE
                     TLAKFEQMKAAKAVAEAQLANILAGTRVETIAARKAEMERAQAALVLAQKTFDRTHTL
                     TEQGNAPQARLDQVTDNLHETERAVDQATSAYQQAVNGYTKEERAIASTNVEKANADI
                     QSVQSIIDQLEVYAPVASQVYQRNVEPGEYVSPGVPLITLIDLADVWIHFD"
     gene            complement(847..1353)
                     /locus_tag="VM910_10145"
     CDS             complement(847..1353)
                     /locus_tag="VM910_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62929.1"
                     /translation="MDPSVISGLAALAGAAIGGLTSAVAAWFTQRTMVQAQWVSGQAL
                     RRQDLYRNFIEVAARCYIHALQHDDPDISGLVDLYADISRMRVVSSSEVIESAERIAQ
                     KVLDTYSEPDKSFGQLRGMVKDHAIDLLHDFSQTCRMEQDFAVGRVGKYASSIPLKRD
                     GRGATIKT"
     gene            complement(1474..1623)
                     /gene="ccoS"
                     /locus_tag="VM910_10150"
                     /pseudo
     CDS             complement(1474..1623)
                     /gene="ccoS"
                     /locus_tag="VM910_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
     gene            complement(1620..2417)
                     /locus_tag="VM910_10155"
     CDS             complement(1620..2417)
                     /locus_tag="VM910_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HVI62930.1"
                     /translation="MTMIPSVAPPLPTPNQWTRPLTSADPFKWLAQGWRDFMIEPAMS
                     VVYGLLIFLISVVFVGGLVALGRDYILFPAFAGFMVIGPILAVGLYEKSRRIETGKPL
                     RWRNLFFVRPRSGGQILFTGVLLCLLMMVWMRAAVIIYALFFGLRPFPGLNHIAPMLF
                     TTGVGWAMLAVGTAVGGLFAAFSFAISAFSIPMLLNEPTDALTAMGSSMALVWNNLRV
                     MLIWAVIVLGLFLASLASAMLGLILIYPLLGHATWHAYRAVQPGAIL"
     gene            complement(2414..2683)
                     /locus_tag="VM910_10160"
     CDS             complement(2414..2683)
                     /locus_tag="VM910_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62931.1"
                     /translation="MNERAANHARAYLNKTTVMRLWLPLALAAAALFAANAQLIYLAT
                     ESEPPCVVHARQGEGSGGHGIFSAAQSSCTPPRAAQAISEQGETR"
     gene            complement(2680..3564)
                     /gene="ccoP"
                     /locus_tag="VM910_10165"
     CDS             complement(2680..3564)
                     /gene="ccoP"
                     /locus_tag="VM910_10165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HVI62932.1"
                     /translation="MAVEQQERDSYTGYLTTGHEWNGIKELNTPVPRAVYFFLTLAFL
                     FSVGYWVLMPAWPLGATYTKGLLGIDQRNVVAASLKQAAVDRSVWTKKIETEDFATIQ
                     SDPGLMQVVRETGHALFGNNCAACHGQDAKGGPGFPNLTTSSWLWGGKPEQIFDTIRV
                     GINSTHPDTHVSQMPAFGRDQMLQRADVLKVDSFVYSLSHPGAKNVDPGNVEAGKAIF
                     AANCVSCHGNDAKGNSELGAPDLTDKFWIYGGDLETIDTTVWGGRQGQMPTWEKRLSE
                     LDRKILTLYLIDKRTANP"
     gene            complement(3555..3725)
                     /locus_tag="VM910_10170"
     CDS             complement(3555..3725)
                     /locus_tag="VM910_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HVI62933.1"
                     /translation="MSVAHDFLVGVSKSFGLFYLIALSVAVLIYTYWPSNKQRFDRAA
                     KSILDDEDTPWR"
     gene            complement(3722..>4066)
                     /locus_tag="VM910_10175"
     CDS             complement(3722..>4066)
                     /locus_tag="VM910_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HVI62934.1"
                     /translation="VVPVSVMPAYDWLLGTELRVDDLSLHLKAQRAVGVPYTDEMIAN
                     APLDAYGQSNPDSPQAEGVTKRYGKATTIRAFDGQPDRLTEMDALVAYLQILGRLTDA
                     AHTYAEQPGAAQ"
BASE COUNT          807 a         1198 c         1247 g          814 t
ORIGIN      
        1 gtcgaagtgg atccagacgt cggccagatc gatcaaggta atgagcggca cgcccggaga
       61 tacatattcg cccggctcca cattgcgctg gtagacctgc gaagcgacgg gggcatagac
      121 ctcgagctga tcgatgatcg attgaacgct ctggatgtcg gcattggctt tttcgacgtt
      181 ggtgctggcg atcgcccgct cttctttcgt gtagccgttg accgcttgct gataggctga
      241 ggtagcttga tcgaccgccc gctcggtttc gtgcagattg tcggtcacct ggtcgagacg
      301 ggcttgcggc gcattgccct gctcggtcag ggtgtgcgtg cggtcgaagg ttttctgcgc
      361 cagcaccaga gccgcctgtg cgcgctccat ttccgccttc cgcgcggcga tggtttccac
      421 tcgcgttccg gcgaggatgt tggcgagttg ggcctcggca acggcctttg cggctttcat
      481 ttgctcgaat ttggccagag tttcgggatt atcgatccgt acgagcaccg cgcctgcagg
      541 aacgttctgg ccacgttcga caggtatttc cttgacccgt ccgtcgacac gcgcggcaat
      601 gtcgaaccgc gtcgcatcgg cctcgccctg cacgagcagt ggttccggcc tcaggagata
      661 gaagatggaa agcgcaacga cgatagctgc gacggtaccg acgataatgg aagggacgcg
      721 cgtcgcagcg cgcgtgttcc gctcctttcc gtcatttggc gcctcggtcg tgctttgagt
      781 ttcattaccc gaggtcggca attccatgcc tgactccgct tggctgatga ccgggatcgc
      841 gctctactat gtcttgattg ttgcgccgcg tccatcgcgc ttcaatggga ttgaactggc
      901 atacttgccg acccgaccaa cggcaaagtc ctgctccatc cggcaagtct ggctgaaatc
      961 atggagcaag tcgatggcgt gatctttgac cattccgcgc aactggccaa agcttttgtc
     1021 cggctccgaa taggtgtcca ggaccttctg cgcgatgcgc tcggcacttt cgatgacctc
     1081 ggacgatgac acgactcgca tccgagaaat gtcggcgtag agatcaacca ggcctgaaat
     1141 gtccgggtca tcgtgttgga gcgcgtggat gtaacatctg gccgcaactt cgatgaaatt
     1201 gcgatagaga tcctgtcggc gcaaagcctg gccggagacc cattgcgctt gcaccatggt
     1261 gcgctgggta aaccaggccg ccacggccga ggtgaggcct ccgatcgccg ctccagccaa
     1321 tgccgcaaga cctgaaatta ccgatggatc catcgttccg aatatcttgc gttatgcgac
     1381 ggcttctgtg tcgtcggcgg ccgtgccggt tttgcgcgtc agcttctgga ccatatcgac
     1441 gttgaccagc cgattcgatc cttcgtcaac cggcagcggc ccttcattgc cctcgaacag
     1501 gatacgttgc gctgcgcctt cgagatcgtc gtattgattg ttccgcaacg tccacatgaa
     1561 tgcggcaagc ccggcgaggc ccatgccgat cgcaatcgga accagaaaga aaaaatcaat
     1621 cataggattg cgcccggttg tacggctcga taggcgtgcc aggtggcatg accgagcagg
     1681 ggatagatca ggatcaatcc gagcatcgcg ctagcaaggc tcgcgaggaa caacccaagc
     1741 acgatgacgg cccagatcaa catcaccctc agattgttcc agaccagcgc catgctggac
     1801 cccatcgcgg tcagcgcgtc ggtcggctca ttgagcagca tcgggatcga aaacgcgctg
     1861 atcgcgaagg agaatgcagc gaataaaccg ccgaccgccg taccaaccgc gagcattgcc
     1921 cagcccaccc ccgtcgtgaa cagcattggc gcaatgtgat tgaggcccgg aaaaggtcga
     1981 agaccaaaaa acagcgcgta gatgatgacc gcggcgcgca tccacaccat catcagcaag
     2041 cacagcagca ctccggtgaa caggatctgg ccacccgatc ggggcctgac aaagaagaga
     2101 ttgcgccagc gcagcggctt gccggtttcg attcgccggc ttttctcgta caggccgacc
     2161 gcaaggatcg ggccgatcac catgaagccg gcgaacgccg gaaacaaaat atagtcgcgg
     2221 ccaagtgcga ccaggccacc gacaaagacg acagagatca agaatatcag cagaccgtag
     2281 accacgctca tcgcaggctc gatcatgaag tcccgccatc cctgggcgag ccatttgaac
     2341 gggtcggctg aggtcagcgg ccgggtccac tggttggggg tcggaagcgg cggtgcgact
     2401 gaagggatca tcgtcatcgc gtttctccct gctcggaaat cgcctgggcg gcccgcggcg
     2461 gcgtacatga ggactgtgcg gcgctgaaga taccgtgtcc cccgctccct tcgccttgcc
     2521 gggcgtgaac aacgcaaggc ggctccgact ctgtcgcgag gtagatcagt tgcgcgttgg
     2581 cagcgaacaa ggccgccgcc gcaagggcga gcggaagcca gagccgcatc acggtcgtct
     2641 tgttcaggta ggcccgcgca tggtttgcgg cgcgctcgtt catgggttcg ccgtccgctt
     2701 gtcgatgagg tagagcgtca ggatcttgcg atcgagttct gatagtcgct tttcccaggt
     2761 cggcatttgg ccctgccttc cgccccagac cgtggtgtcg attgtctcga gatcgccgcc
     2821 atagatccag aacttgtcgg tgagatcggg cgcgcccagt tcggaattgc ccttggcgtc
     2881 gttaccgtga caggagacgc agttggcggc gaaaattgcc ttgccggcct cgacgttgcc
     2941 gggatcgacg tttttcgcgc ccggatggga gagcgaatag acgaacgagt cgactttcag
     3001 aacgtcggcg cgctgcagca tctgatcgcg accgaacgcc ggcatctgcg acacgtgcgt
     3061 atcgggatgt gtggagttga tgccgacacg aatggtatcg aagatctgtt ccggcttgcc
     3121 gccccagagc catgacgaag tcgtcaggtt cgggaaaccg gggccaccct tggcatcctg
     3181 gccgtggcag gcggcgcaat tgttcccgaa cagcgcatgt ccggtctcac gcacgacttg
     3241 catcaagccc ggatcggact ggatcgtcgc aaagtcttcg gtttcgatct ttttggtcca
     3301 gacgctgcga tcgacggctg cctgtttcag cgaagccgcc acgacgttac gttgatcgat
     3361 gccgagcaga cctttggtat aggtcgcgcc cagcggccat gccggcatca gaacccagta
     3421 tccgactgaa aacaggaagg cgagggtcag gaagaaatag accgcacgcg ggaccggcgt
     3481 gttcaattcc ttgatgccgt tccattcatg gccggtggtg agataaccgg tgtagctgtc
     3541 acgctcttgt tgctctaccg ccatggcgtg tcctcatcgt caaggatgga cttggcggcg
     3601 cgatcaaacc gctgcttgtt ggacggccaa tacgtgtaga tcaagacagc gaccgacagc
     3661 gcgatcaggt agaacaaccc gaacgacttg gagacgccga cgagaaaatc gtgcgcgaca
     3721 ctcattgagc cgctcctggc tgctctgcat atgtatgggc cgcgtcggta aggcgaccga
     3781 gaatttgcag ataggcgaca agcgcatcca tttccgtcaa ccgatccggc tggccgtcga
     3841 atgcgcggat ggtcgtggcc ttgccatagc gtttggtcac tccttcggcc tgcggcgagt
     3901 ccgggttgga ctggccatag gcgtcgagcg gtgcgttggc gatcatctcg tcggtatagg
     3961 gcacgccgac cgcgcgctgg gccttcagat gcagggacag atcgtcgacg cgcaattccg
     4021 tgccgagcaa ccagtcatag gcgggcatga ccgagaccgg cacgac
//
LOCUS       DATLJT010000439         4665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2092924,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000439 DATLJT010000000
VERSION     DATLJT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092924"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1190
                     /locus_tag="VM910_10180"
                     /pseudo
     CDS             198..1190
                     /locus_tag="VM910_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
     gene            1298..1615
                     /locus_tag="VM910_10185"
     CDS             1298..1615
                     /locus_tag="VM910_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HVI62935.1"
                     /translation="MTSPLEQKKIRIAGPSIVTANRTWDGAVIYRTAQQGWSAELSDA
                     VIVRSNDEARALLAESVADDLGAVGAYIAPVEITEGGTVEPGNLREQIRRSGVTIGLP
                     VQA"
     gene            1628..3283
                     /locus_tag="VM910_10190"
     CDS             1628..3283
                     /locus_tag="VM910_10190"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVI62936.1"
                     /translation="MYAYDEIDRTLINERVSEFRDQVKRRLSGELTEDEFKMLRLQNG
                     VYLQLHAYMFRVAIPYGTLSSRQLRRLAHVARKYDRGYGHFTTRQNIQFNWIKLAELP
                     DALADLAEVGIHAMQTSGNNTRNVTSDQWAGVAPGEVEDPRIWAEILRQYTTLHPEFS
                     FLPRKFKFAISASPHDRAAIKIHDIGLRLHKNDKGETGFEVLVGGGLGRTPFIAKTIK
                     PFVHGRDLLSYVEAIMRVYNQYGRRDNIYKARIKILVHELGIEKFAAEVEEEWQQMRD
                     GALKLDDDVVEDIRARFFYPNYEKLPHMSDELRKAAQDQRFEAWRRNSVFMHKVQGYA
                     IVTLSLKPVGGPPGDATADQMDALADIADKYSFGEIRVGHEQNLALPHVARRDLPALW
                     KALDKIGLATPNVNLISDIIACPGLDYCSLANARSIPIAQELTRRFANHDTANLIGRL
                     HINISGCINACGHHHVGHIGILGVEKNGEEFYQITIGGRADENAELGTLIGPSVKFEE
                     VADVVEDIVEAYLALRATPDELFIDTVKRLGIEPFRERVYATR"
     gene            3270..3785
                     /locus_tag="VM910_10195"
     CDS             3270..3785
                     /locus_tag="VM910_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HVI62937.1"
                     /translation="MPLVKGGNIADDAFVHLADDAELPDAGDVLVSAARFLHDPEALL
                     RRPGKVGVTWPNSRDVDDLVPYLGRLGTVALVFPTFRDGRAYSQARLLRERFSYRGEL
                     RATGQVLRDQFVFMLRAGFDAFEVKKQNDAEAFTQTVKRYSVFYQPTGDGRLTALHRR
                     MQLRHSESAGL"
     gene            3782..4513
                     /locus_tag="VM910_10200"
     CDS             3782..4513
                     /locus_tag="VM910_10200"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HVI62938.1"
                     /translation="MIALTQDVSLKPEGAGLPPVEALARALTNASPASIIAKALQIFG
                     REKLALVSSFGTESAALLKVMADVDPAIPVIYLDTGWLFEETLAYRDTLTAKLGLKDV
                     RSIKPLEETLSREDPERELWFSDPDACCRIRKVEPLARALKPFAGWINGRKRFQGGLR
                     ADIPVVEQDGARLKFNPFANVSREEIEAIFKRADLPSHPLLAKGFLSVGCMPCTSRAQ
                     AGEDARAGRWRGRGKTECGIHTTAS"
BASE COUNT          838 a         1473 c         1477 g          877 t
ORIGIN      
        1 gtgctgtgcc ggacgcaacc ggtctttgac ggtgtctttg ttgagaagat gcatcttccg
       61 acccttggtc tccggaggtg aaaattccaa agttgcagcg cagcagagaa gaaaaaattt
      121 tctctttgtg ggcgttgtgc gagacatata tagaaaataa ttccagtcaa ccccgaagat
      181 ggggaagcga gtgtcgcatg cgctatctgc cggtgttcct cgatctgcac agcggcccgg
      241 tgcttttatt gggcagcggt gagctcgcgc gcgccaagtt gcgcgtgctg ttgtctgccg
      301 gcgcgcgcat ccgttggtat gcgtctgctg tgcacgacgt gcaggggctc gagcccgcgc
      361 agcttgcgcg gatcgagttt gccaaaggcg atccgctgca agccgatctt gccggcatcg
      421 tcgcgatctt ttgtgcaggc gccggcgaag caggcgtggc gatgtcgctg cgcgcaaagt
      481 ccgtcggtct gcccgtcaat gtgatggacg atcttgcgca ttcgaccttc atctttcccg
      541 ccatcgtcga tcgcggagat gtcgtggtgg cagtcggcac cggcggaacg tcgccggtgg
      601 tggcgcggcg cgtgcgcgag aagatcgagg cggtgttgcc cgcgcgcatc ggggagctcg
      661 cgaaattcat cgggcgcttc cgcaaggcga ttcacggccg cattgacgaa atggcactac
      721 gccgacgctt ctgggaacgc gtgatcgacg gcccgatcgg cgcgctggtt ctctccgggc
      781 acagggacga ggccgaagct gcgttgaacg cgattgccga tccgaacctg ttcgccggtg
      841 ccctcgcatc cggtcgactg caaggcagcg tgacgttcgt aggcgccggt cccggcgatc
      901 ccgatctttt gacggtgaag gcgctgcgca ttctgcagga cgccgatctc gccgtccacg
      961 accaggccgt gtcgcccgaa attctggatc gcatccgtcg cgacgcgtcg cgcatcgtcg
     1021 tcggcacgag caaggacgac gccaatcgcc agatgattga agcggcgcaa tccggccagc
     1081 gcgtggtgcg gcttgccggc ggcgatggcc tggcgccggg cttgagcgag gagctcgccg
     1141 cgctgcgcga atccggcatc acctgtcttg tcgtgccggg cgtcaccgcg gagttcgctg
     1201 atgccaggac atttccaccg ccggcgcggt cacgcggttc gcgcgccggg gcagccaaaa
     1261 ccctcacctc ttagtcgcac acttgttgga agcagcaatg acctctcccc tcgaacagaa
     1321 aaaaatccgc atcgcaggcc cctcgatcgt gacggccaac cgcacctggg acggcgcggt
     1381 gatctatcgc accgcgcagc agggctggtc ggccgagctc tcggatgccg taatcgtgcg
     1441 cagcaacgat gaagcgcgcg cgctgcttgc cgaatccgtc gccgacgatc tcggcgcggt
     1501 cggcgcctac atcgctccgg tcgagatcac cgagggcggg acggtcgagc caggcaatct
     1561 gcgcgagcag atccgccgct ccggcgtcac catcggtctt cccgtccagg cgtaaggacg
     1621 cgtttccatg tacgcttacg acgaaatcga ccgcacgctg atcaacgagc gcgtctcgga
     1681 attccgcgac caggtgaagc gccggctttc cggcgaactg accgaggacg agttcaagat
     1741 gctgcggctg cagaacggcg tctatctgca gctccacgcc tacatgttcc gtgtcgcgat
     1801 cccctacggc acgctgtcgt caaggcagct acgccgcctc gcccatgtcg cgcgcaaata
     1861 tgaccgtggc tacggccatt ttaccacgcg ccagaacatc cagttcaact ggatcaagct
     1921 tgccgagctg ccggatgcgc tcgccgatct cgccgaggtc ggcattcatg cgatgcagac
     1981 ctccggcaac aacacgcgca acgtcacatc cgatcaatgg gcgggcgttg cacctggcga
     2041 ggtcgaagat ccccggatct gggcggaaat cctgcggcaa tacaccacgc tgcacccgga
     2101 gttctcgttc ctgccgcgca agttcaagtt cgcgatcagc gcgtcgccgc acgaccgcgc
     2161 cgcgatcaag atccacgaca tcggcttgcg cctccacaag aacgacaagg gcgagaccgg
     2221 attcgaggtg ctggtcggcg gcggcctggg ccgtacgccg ttcatcgcca agaccatcaa
     2281 gccgttcgtg catggccgcg atctcttgag ctatgtcgaa gcgatcatgc gcgtctataa
     2341 ccagtatggc cgccgcgaca acatctacaa ggcgcgcatc aagattctgg tgcacgaact
     2401 cggcatcgag aagttcgcgg ccgaggtcga ggaagaatgg cagcagatgc gcgacggcgc
     2461 gctgaagctc gatgacgacg tggtcgagga catccgcgcg cgcttctttt atccgaacta
     2521 cgagaagctg ccgcacatgt cggacgagct ccgtaaggcg gcgcaggacc agcggttcga
     2581 ggcgtggcgg cgcaactccg tgttcatgca caaggtgcag ggttatgcga tcgtgacgct
     2641 ctcgctaaag cccgtgggcg gcccgcccgg agacgccact gccgaccaga tggatgcgct
     2701 cgccgacatc gccgacaaat attctttcgg cgagatccgt gtcggccatg aacagaatct
     2761 ggcgctgccg catgtcgcca ggcgcgacct gccggcgctg tggaaggcgc tcgacaaaat
     2821 cgggctcgcc acgccgaacg tcaatctgat atccgacatc atcgcctgcc ccgggctcga
     2881 ttactgctcg ctggcgaatg cgcgatcgat tccgatcgcg caggaactga cacgccgttt
     2941 cgccaatcac gacaccgcaa atttgatcgg ccggctgcac atcaacattt ccggttgcat
     3001 caatgcctgc ggccatcacc atgtcggcca tatcggcatt ttgggtgtcg agaaaaatgg
     3061 cgaggaattt taccagatca cgatcggcgg ccgcgccgac gagaatgccg agcttggcac
     3121 gttgatcggg ccctcggtga agttcgagga ggtcgccgat gtcgtcgagg acatcgttga
     3181 agcctatctc gcgctgcgcg caacccccga cgagcttttc atcgataccg tgaagcggct
     3241 cggcatcgaa ccgttcaggg agcgcgtcta tgccactcgt taagggcgga aacatcgccg
     3301 acgatgcgtt cgtccatctc gccgatgacg cagagcttcc cgatgccggc gacgtgctgg
     3361 tttcggcggc gcgctttctg cacgatcccg aggcgctgct gcgccgtccg ggcaaggtcg
     3421 gcgtgacctg gccgaacagt cgcgacgtcg acgatctcgt gccttatctc ggccggctcg
     3481 gcacggtcgc gctggtgttt ccgactttcc gcgatggccg cgcctacagc caggcgcggc
     3541 tgttgcgcga gcgcttttcc tatcgcggcg agctgcgcgc cacgggacag gtgctgcgcg
     3601 accagttcgt gttcatgctg cgcgccggct tcgatgcgtt cgaggtgaag aagcagaacg
     3661 atgccgaggc cttcacccag accgtgaagc gctattcggt gttctatcag ccgaccggtg
     3721 acggccgtct cacggcactg catcgccgta tgcagcttcg ccattccgaa agtgcaggcc
     3781 tatgattgcg ctcacacagg acgtttcgct caagcctgaa ggggctggtc tgccgccggt
     3841 ggaagcgctt gcccgcgcgc tcacgaatgc ctcgcccgcg tcaatcatcg cgaaggcgct
     3901 gcagattttt ggccgggaaa agctcgcgct ggtctcctcc ttcggcacgg aatccgctgc
     3961 gctgctcaag gtgatggcgg atgtcgatcc tgcgatcccc gtgatctatc tcgataccgg
     4021 ctggctgttc gaggaaacgc tggcctatcg cgatacgctg accgcaaagc tcgggctcaa
     4081 agatgtgcga tcgatcaagc cactggagga gaccttgtcg cgcgaggatc cggagcggga
     4141 actctggttt tccgatccgg atgcctgttg ccgcatccgc aaagtggaac ctctggcgcg
     4201 ggcgctgaag ccgtttgcgg gctggatcaa cgggcgcaaa cgtttccagg gcggcttgcg
     4261 cgccgacatt ccggtcgtcg agcaggacgg ggcgcggctg aagttcaatc ccttcgccaa
     4321 cgtctcgcgc gaagagatcg aggcgatctt caagcgcgcc gacctgccgt cacatccact
     4381 gttagccaaa gggttcctct cggtcggctg catgccctgc acgagccgcg cgcaggcggg
     4441 cgaggatgcc cgcgccgggc gctggcgcgg ccgcggcaag acggaatgcg gcatccacac
     4501 cacggcgtca taactcgtcc ctcacagggc cggtaacgcg gctctgtcgg ttttcgtagc
     4561 gaaaatcggc ggaaaattca cctttcggcc acggttggta tgcaacaacg gccgctcttt
     4621 tgggggtggg cctgttcatg cgcgcggcgg ccgcgatctt tttca
//
LOCUS       DATLJT010000440         1591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1862890,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000440 DATLJT010000000
VERSION     DATLJT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862890"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1237
                     /locus_tag="VM910_10205"
     CDS             251..1237
                     /locus_tag="VM910_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62939.1"
                     /translation="MPALRRREFITLLGGAAATWPLSAQAQQPAMPVIGFFRSTPAEP
                     FASLVPAFRQGLNETGFVEGQNVVVEQRWADNQLNRLPALAADLVRRQVAVIVSNGPA
                     VEAARSATTTSPIVFVIGDDPVRRGLVTSLNRPGGNLTGVTFFANRLSAKRLEMLLEL
                     VPRARVVAVLIDPNFSSSADELRELEEAGRTIERKIVAVKATNEREFDRAFTRIIEAG
                     AGGLVLCGSPFFSSESRTLIALAARHAIPTIYDIRDYPAAGGLISYSASFTDAYRQAG
                     VYAGRILKGAKPSELPVLQPTTFELVINLKTAKVLGLTVPLTLQAAADEVIE"
BASE COUNT          309 a          495 c          510 g          277 t
ORIGIN      
        1 cattgggaaa tgttcgcacc agcactgcga gagcctggtc agagccagaa tggcataagc
       61 tcatctccga gctgccgtga agatcgcaga caagaagcgc gacaaagaaa gaatgaacct
      121 gcgaccgacc gctgcggccg accgcctgcc ggcgtccccc caattcagaa cgattcaggg
      181 ttgccccaag gaccggccgg tcccgccgtg ctaggctgag gaaaccggct gcactgggtg
      241 gggagagacc atgccggccc tgagacgacg cgagttcatt accctgctcg gcggcgcggc
      301 ggcaacatgg ccgctcagcg cgcaggcgca gcaaccggcg atgccggtga tcggattttt
      361 ccgcagcaca ccggctgagc cgttcgcgag tctcgtgccg gcgttccgcc aaggcctcaa
      421 cgagacgggg ttcgtggaag gtcagaacgt ggtcgtcgag caacgctggg ccgacaatca
      481 gctcaatcgg ctgccggccc tggcggccga tctggtccgt cgccaagtgg cggtgatcgt
      541 cagcaatggc ccagcggtgg aggcggcccg gagcgccact acgaccagtc caatcgtgtt
      601 cgtgatcggt gacgatccgg tgagacgagg cctcgtcacc agcctcaatc ggccgggcgg
      661 caacctgacg ggcgtgacgt tcttcgccaa ccggcttagc gcaaagcgcc tggagatgct
      721 gcttgagtta gtgccccggg ctcgcgtcgt tgcggtactg atcgatccga atttttcttc
      781 atcagccgac gaattgcgtg aattggagga ggcgggtcgc accatcgagc ggaaaatcgt
      841 ggcggtgaag gccacgaatg aacgcgaatt cgatcgtgcc ttcacaagaa ttatcgaggc
      901 cggcgccggc gggctcgtgc tctgcggaag cccattcttc agcagcgaaa gtcgaactct
      961 catcgcactg gccgcgcgcc atgccattcc gaccatctac gacatccgcg actaccccgc
     1021 agctggcggt ctcatcagct attcggcaag cttcaccgac gcctatcgac aggcgggcgt
     1081 ctatgccggg cgaattctca agggagccaa accgtcggag ctccccgtgc tgcagccgac
     1141 caccttcgaa ctggtgatca acctcaaaac cgccaaggtg ctcggcctga cagtgccgct
     1201 gacgctgcag gccgcggccg acgaggtgat tgagtagcga ttttttgctg cgctgcgttc
     1261 ggtcgctatt ggcacaaagc ggactttcgg agaggtgcgc tgtgatgtcc gcttttgggg
     1321 gcaagcggac atgccgcgac cgcgggtggc gcatcggccg ggtcgctaaa acccaaagca
     1381 gtcacaagcc ctcactgcga caagctggag ccgccacgcg gcagcacttt gtccaacgtc
     1441 aggctgttgt gtcctgatga agctcaccgc ctcgcataag ccgccgggcg atgtcggccg
     1501 tttgcagctc ggcgcgttcc gtaggcgaat gggcagcgag atagcgggcg acagcgatga
     1561 ggatgtgccg accctcgtcg gtattgcccc a
//
LOCUS       DATLJT010000441         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2093095,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000441 DATLJT010000000
VERSION     DATLJT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093095"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VM910_10210"
     CDS             <1..331
                     /locus_tag="VM910_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62940.1"
                     /translation="EAITTVETTKERWFEAEANRIAGEIALLLPEPDVAKAEACFERA
                     LAVARKQQAKSWELRAAMSMARIWRDQGRRDEARDLLAPLFGWFTEGFDTRDLKGAKL
                     LLDELHA"
     gene            491..718
                     /locus_tag="VM910_10215"
     CDS             491..718
                     /locus_tag="VM910_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62941.1"
                     /translation="MRGKTVVITGGTSGIGEVAAELLAKKGARFVLVARDKTRGDAIL
                     VRLRNCAPGVAPSLYFADTPCRDETRGGSNH"
     gene            1216..1482
                     /locus_tag="VM910_10220"
     CDS             1216..1482
                     /locus_tag="VM910_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62942.1"
                     /translation="MAAQRDIGGHEGKWFAAALTSRIIKGSSRPHFQPDADLSGIFGE
                     MNTGVLKSFLYFEDGCEIPFHCSLGLFGPSQSCKTDAGLTGAAN"
     gene            1812..2645
                     /locus_tag="VM910_10225"
     CDS             1812..2645
                     /locus_tag="VM910_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HVI62943.1"
                     /translation="MSETPLKLAKTLGRRFFIGGSDARIIMGDDEAALVRLWREKRGE
                     AESEDLSGNLIVQLGRATEELNRSWYERNSGRQVRDVQRRVRHSAIPWMAATLDGIVE
                     GTEAVFEAKFMLPWSFSEEGAAEKYMAQVQHNMWVTHLRTSVLSIITGGGKWVEIAIP
                     MDPLYLAVLVSAEKKFWRCVQSGEVPHLVMAEPPRPRIEAIRIVDMSSSNSWAEFAAL
                     FCNTREAFLDHERAKSELKALMPEDAKEAIGHGVRGKRSKSGAVSFDVLTTEAVHAPV
                     Q"
BASE COUNT          521 a          753 c          829 g          557 t
ORIGIN      
        1 tgaagcaatc actactgtgg aaacaaccaa ggaaaggtgg ttcgaggctg aggccaatcg
       61 cattgccggg gaaatcgcgc tgctgttgcc ggagccggat gtggcgaaag ctgaagcgtg
      121 tttcgagcgt gcgcttgcgg ttgctcgtaa acagcaggca aagtcctggg aattgcgtgc
      181 ggcgatgagc atggcgcgga tctggcgcga tcaaggcagg cgggatgagg cccgcgatct
      241 tcttgctccg ctcttcggct ggttcaccga aggcttcgac acccgtgact tgaagggggc
      301 caagctcttg ctcgatgagc tacacgcctg acaactaatt tcgaaaccgc ctcttagaaa
      361 ggctgcagca agatcgcggc aaggcgcctc cagaccgcca tgaggatcga cagactggct
      421 gggtggcgaa ccaatcgacg acaccgtcgc cagagatcat tgaggttaga agtacggctc
      481 gagagcgctt atgagaggta agactgttgt catcactggt ggcacgtccg gcattggaga
      541 ggtagccgcg gaactactcg ccaaaaaggg cgcccgtttc gtcttagtcg cacgggacaa
      601 gacccgcggc gacgcgattc tcgtgcgact gcgcaactgc gcccctggcg tcgccccctc
      661 cctgtatttt gccgatacgc cttgccgaga tgaaacgcgt ggcggctcaa atcactgacc
      721 aggagcagcg tatcgatgtc gtgatcaata acgcgggcgc actttgcgcg gcggatgctt
      781 gcgcaggacg ggcttgaatt cacattcgcg ctcaaccaca tggcgtattt cgtcctgact
      841 gagggattgc gcgagtggct cttagcctcg agacccgccc gcatcatcaa cacggcttcc
      901 gccgcgcact aggacgccac ctcggatttc gatcttcaat cggcgaatac cgggccttga
      961 aagcctaaag tcgctcgaaa ctgtgcaaca tcctgtttac gagcgagctc gcccgccggc
     1021 tgcgcggcac ggcgagtcac cgcaaattgc ctgcatcccg gcttgtcgct acacgctttg
     1081 gtgaccaaag cggggacccg attgcgcacc tgccctgggt cggactgtgg ctaagaccac
     1141 ggggcaatac ttttacgagt gtctcccgac cacgccgtcg tcgggtgcac gcgacgatcg
     1201 atctgctttc ttgttgtggc agcgcagcgc gacattggcg ggcatgaagg aaaatggttc
     1261 gccgccgctt tgacttcgag gatcatcaag ggatcatcgc gtcctcactt tcagccggac
     1321 gccgatctct ccggtatctt cggtgaaatg aataccggcg tgctcaagag ctttttgtat
     1381 tttgaggatg gttgcgaaat tccttttcac tgttccctcg gtttgttcgg tccgtcgcag
     1441 agttgcaaga ccgatgccgg ccttacgggg gcggccaatt aggacttttt ccgtgggtgc
     1501 tttcgcagcc gcactccagg gccaccgcca ttgtcgtcta agaactcgac gcctgcagat
     1561 tccagcgctg accgtatttt ggacccggtt tcgttccggc cgcctagcgg gccgtcttgg
     1621 gcctctagtc gcttaatcgt cgggatcgag acgtctgcgg cagccgctag ccgctcctgt
     1681 gaccaggcga gaagtgcccg tgcagcctta atctgcctaa ttgatacttt tagtattgac
     1741 ttttgtggca ttggttgcga tacttttagt atcgactggg ttgaggccca atgattgttg
     1801 gagattatcc tatgtcggaa actcccttga agttagcgaa aaccctaggg cggcgctttt
     1861 tcatcggcgg ctcggatgcc cggatcatca tgggtgacga tgaggccgcc ctggtgcgcc
     1921 tgtggcgcga aaaaaggggc gaggccgagt cggaggacct ctcgggcaac ctcatcgtgc
     1981 agctcgggag agcaaccgag gagctcaatc gctcgtggta tgagcgcaac agcggccggc
     2041 aggtccgcga cgtgcagcgc cgggtcaggc attcggcaat tccttggatg gcagcgaccc
     2101 tggatgggat tgtggaaggc acggaagccg tgttcgaggc caagtttatg ctgccctggt
     2161 cattctccga agagggagcg gccgaaaaat acatggccca ggtccagcac aacatgtggg
     2221 tgacccattt gcggacctcg gtgctctcca tcattaccgg tggcgggaaa tgggtcgaga
     2281 tcgccattcc gatggacccg ctctatctcg ccgttctggt gtcggcggaa aagaagttct
     2341 ggcgctgcgt ccaatcgggc gaggtcccgc acctcgtcat ggccgagccg ccaaggccgc
     2401 gcatcgaagc gatccgcatc gtcgatatga gctcgtcgaa ctcctgggcg gagttcgcag
     2461 cgctcttctg caacacccgg gaggcctttc tcgaccacga gcgggcgaag agcgagctaa
     2521 aggctctgat gccggaggat gccaaggagg cgatcgggca cggggtgcga gggaagcgtt
     2581 ccaagtcagg ggcagtcagc ttcgacgtcc taacgacgga ggcggttcat gcaccggtcc
     2641 agtgaatcag tggcggcgct
//
LOCUS       DATLJT010000442         3867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_25599,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000442 DATLJT010000000
VERSION     DATLJT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25599"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VM910_10230"
     CDS             complement(<1..321)
                     /locus_tag="VM910_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62944.1"
                     /translation="MRDWSKAEWVDPTYNWKPLPVPEMPDRLLIDYATRCNLRCHMCP
                     VWGLDDDNAIDNVKGIMDLAAARKMLDEFTNAQPMVAPSIYGEPLLIPNLREVLSDLK
                     RRGIA"
     gene            518..976
                     /locus_tag="VM910_10235"
     CDS             518..976
                     /locus_tag="VM910_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI62945.1"
                     /translation="MRTGHAVAALLVLEDGRYIMQLRDDIPNIWYPGHWGLFGGGVDD
                     REGEVAALRRELREELELDVNDARLFAIINYDLQPIGLDCYFRRYYEVPVTIAAWNRV
                     VLHEGSEVRALTGDEALSLPRISPYDAFALFLHHNRKRLAGSIYAPSPPE"
     gene            1100..1894
                     /locus_tag="VM910_10240"
     CDS             1100..1894
                     /locus_tag="VM910_10240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI62946.1"
                     /translation="MTPDYATARADIIDGIRAFRVWTRLGWQEVKRRYRRTFFGPFWA
                     TLSIGAFIGGMVFIWAPLFKTDVSSYLPFLATGLVSWTFATALISEGCGTYTAGVALI
                     TQLKFPYTVLNFMVVWRNVIVFFHNVVIVVIVVIALRIPVNWHTLLLFPGIIIVAVNG
                     AWMTILFGMVSARFRDVPPLVGNVIQILMFVTPIFWFSSQLGPGAQPIVTYNFMYHLV
                     EVMRAPMLGMAPSLLSYAFTIAGAIVGWLVTFDLYARFRRRIPYWL"
     gene            1909..2679
                     /locus_tag="VM910_10245"
     CDS             1909..2679
                     /locus_tag="VM910_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI62947.1"
                     /translation="MTSIVASNLTIDFPIYGSSHRSLRQTLFARTGGLIRHERAGRQQ
                     RVVVRALDDVSFTLRNGDRLGLIGHNGAGKSTLLKVIAGVYTPDAGSIRIEGRVSPLF
                     NSAPGLDPDDNGYENIKTCGMFLGMSSEEIDRKLLDIVDFCELGEYLDLPVRTYSTGM
                     VARLGFAIATAIEPDILLLDEGLGAGDARFAARAEARMQSLIQRTRILVLASHSDAMI
                     KSMCNRAILLEKGRLIAIGPVDEIINRYHQRPAEVHVA"
     gene            2833..3528
                     /locus_tag="VM910_10250"
     CDS             2833..3528
                     /locus_tag="VM910_10250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HVI62948.1"
                     /translation="MKFSDISIDFFENTSTEPAFLHGIIRLNAGARIVKSTIRGPLFL
                     NKNSQIGPDVVVGKYFGMNESCFVARATVGSFGAIGARTAINPFNHPTSWLSTNEFQY
                     HPKSFDWVDEYNDFVRLERTPDMFKHVTIGNDVWTGHNVNVMAGVNIGDGVVIAAGSV
                     VTKDVPAYAIVGGIPATLIRYRFSEKTIERLLCVKWWELELSQLSGLPFRDIDRCLDL
                     IEEIKSKAPAAAE"
     gene            3532..>3867
                     /locus_tag="VM910_10255"
     CDS             3532..>3867
                     /locus_tag="VM910_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HVI62949.1"
                     /translation="MATGREAAAVVPILERFGVPRDGALVVHSAIAPLSRRGLRAEAI
                     IEALIEYMRGGNLLMPTMTWRTVTPEQPHWDELETPSHTGVLTEIFRTRYASGRSIHP
                     THSVAGLGPA"
BASE COUNT          718 a         1183 c         1119 g          847 t
ORIGIN      
        1 ggcgatgcca cgtcgcttca gatcggagag gacctcgcgc agattcggga tcaggagagg
       61 ctcgccatag atactcggcg ccaccatcgg ctgggcattg gtgaattcgt cgagcatctt
      121 gcgcgcggcg gcaaggtcca tgatgccctt gacgttgtcg atggcattgt cgtcgtcgag
      181 gccccaaacc gggcacatgt ggcagcgcag gttgcagcgc gtggcatagt cgattaggag
      241 acgatccggc atttccggaa ccggcagcgg cttccaatta tacgttggat cgacccactc
      301 ggccttcgac cagtcgcgca tggcacccct taacccattg aattgtggca gcattcctgg
      361 ctgcgacgga ttccgcctgt gcagccggtc ttataaccgc cccgatcata atgacaagcg
      421 gttttacatg tatattgccc acgctcgccc gcggccatag ccacaacgaa ggcccctact
      481 cttgactgtt gcaaactttc tggcgtcgcg cgccccattg cggaccggtc atgccgtcgc
      541 agcgctgctc gtgcttgagg atggtcgcta cataatgcag ttgcgcgatg acattccgaa
      601 tatttggtac cccggccact ggggcctgtt cggcggcggc gtcgacgatc gagagggaga
      661 agtcgcagcg ctgcgtcgag agcttcggga ggagctcgag cttgatgtga acgacgcccg
      721 cttgttcgcg atcatcaact atgatctgca gccgatcggt ttggattgct atttccgaag
      781 gtactatgag gttcctgtga ccatcgccgc gtggaaccgt gtcgtgttgc acgaaggcag
      841 tgaggtgcgt gcgctgaccg gcgatgaagc cctgtcgctt cctcgcatct ccccgtacga
      901 tgcgtttgcc ttgttcctgc accacaatcg aaaacggctc gcgggctcga tttatgcgcc
      961 ttcgccgccc gaatgatccc tccggcgccg agcccggcag gatcggtact gtattgcctc
     1021 atagcagctt cgatggtaac cccgattcga accctgcgga cgccgttcgc gttcagctgc
     1081 ccttttcgat gagcttgtcg tgacaccaga ctacgccacc gcgcgagccg acattatcga
     1141 cgggatccgc gcattccgag tctggacccg actcggctgg caagaggtca aacggcgtta
     1201 ccggcggacg ttttttggac cgttctgggc gacgttgagc atcggcgcct tcatcggtgg
     1261 aatggtcttc atttgggcgc cgctattcaa gaccgacgtc agctcctacc tgcctttcct
     1321 ggccactggt ctcgtaagct ggacctttgc gaccgcactt atttccgagg gctgtgggac
     1381 ctacacggcg ggcgtcgctc tcatcacgca gctgaagttt ccgtacaccg tgttgaattt
     1441 catggtcgtg tggaggaacg tcatcgtgtt cttccacaac gtggttatcg ttgtcatcgt
     1501 cgtcatcgct ctgaggatcc cagtcaattg gcacacctta ttgctgttcc ccggtatcat
     1561 tatcgtggcc gtgaatggag cgtggatgac gatattgttc ggcatggtga gcgcgcgctt
     1621 tcgtgacgta cccccgctcg tcggcaacgt cattcagata ttgatgttcg tgacgccgat
     1681 attctggttc tcgtctcaac taggtccggg agcacaacca atcgtcacgt acaacttcat
     1741 gtatcatctg gtcgaggtga tgcgcgcgcc aatgctcggc atggctccgt cgcttctgag
     1801 ctacgccttc accattgccg gcgcgatcgt cggatggctc gtgaccttcg acctctacgc
     1861 ccgctttcga cggcgcattc cgtattggct ctgatggggt attccacctt gacctcgatc
     1921 gttgcgagta accttacgat cgatttcccg atctacggat cgagtcatcg cagcctccgc
     1981 caaaccctgt tcgcccgcac cggagggcta attcggcacg agcgcgcagg tcgccagcaa
     2041 cgcgtcgtcg tgcgcgctct cgatgatgtc tcgttcaccc tgcggaacgg tgatcgcctc
     2101 gggctgattg ggcataacgg ggcgggtaag tccacactac tgaaagtcat tgccggagtg
     2161 tacacgcccg acgctggctc catccgcatc gaaggccgcg tttccccgct gttcaactcc
     2221 gcacccggcc tcgaccccga cgacaacggc tacgaaaata tcaagacgtg cggcatgttt
     2281 ctcgggatga gttccgagga gatcgaccgc aagcttctcg acatcgtcga tttctgcgaa
     2341 ttgggtgaat acctcgacct tccagttcga acctattcga ccggcatggt tgcgcgcctc
     2401 ggctttgcga ttgcaactgc aatcgagcca gacattttgc tgctcgacga agggttgggc
     2461 gccggggatg ctcgatttgc ggcgcgcgcc gaggcccgca tgcaatcgct gatccaacgc
     2521 acccgcatcc ttgtacttgc cagtcactcc gatgcgatga tcaaatcgat gtgcaaccgg
     2581 gcaattctct tggaaaaggg ccgcctgatt gcaataggac cggttgacga gatcatcaat
     2641 cgctatcacc agcgtccggc cgaagtccac gtcgcgtagg ccacccctgc gcatcgagca
     2701 ggatcgagtt cgcgctggca cgttgctgca agcgcttggg attgataatt tcgttccaac
     2761 cgtgccatca agaggaaatg ccgcgagatt tgggtcgaag ttctcggtcg gcctcgacgc
     2821 gcctaaaccg acatgaagtt ttccgacatt tcgatcgact tctttgagaa taccagcacc
     2881 gagcctgcat tccttcatgg catcatccgg ctcaacgctg gcgcccgcat cgtcaagtcc
     2941 acgatccgcg gtccattatt cctaaacaag aattcgcaga tcggccccga cgtggtcgtt
     3001 ggtaaatatt ttggtatgaa cgagagttgc ttcgttgcgc gcgcaaccgt cggctccttc
     3061 ggggcgatcg gcgcgcgtac cgcgatcaat ccgttcaacc atccgaccag ctggctcagc
     3121 accaacgagt tccaatatca ccccaagtcg ttcgattggg tcgacgagta caacgacttc
     3181 gtccggctcg agcgcacgcc cgacatgttc aagcacgtga ccatcggcaa cgatgtgtgg
     3241 accggtcaca atgtcaatgt catggctggc gtcaacatcg gcgacggcgt ggtgattgcc
     3301 gcggggtcgg tcgtgaccaa ggatgtgcct gcctacgcca tcgtcggcgg catccctgcc
     3361 acgctgatcc gataccgatt ttccgagaag acgatcgagc gtctgctgtg cgtgaaatgg
     3421 tgggaactcg aactctcgca gctcagtggc cttccgttcc gcgacatcga ccgctgcctc
     3481 gacttgatcg aggagatcaa gtcgaaggca cccgcggcgg ccgagtgaac catggcgacg
     3541 ggccgagagg ctgccgccgt ggtgccgatc ctggagcgct tcggcgtgcc gcgggatggc
     3601 gccctcgtgg ttcacagcgc gatcgcgccg ctgagccgac gcgggcttcg cgccgaagcg
     3661 attatcgaag ccctgatcga atacatgcgc ggcggcaacc tgttgatgcc gaccatgacg
     3721 tggcgcacag tgacgcccga gcaaccgcat tgggacgagc tcgagacgcc gtcgcatacc
     3781 ggggtgctca ccgagatatt ccgcacccgc tacgccagcg ggcgcagcat tcaccccacg
     3841 cattcggtgg cggggctcgg accggcg
//
LOCUS       DATLJT010000443         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3931020,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000443 DATLJT010000000
VERSION     DATLJT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3931020"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..927
                     /locus_tag="VM910_10260"
     CDS             271..927
                     /locus_tag="VM910_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI62950.1"
                     /translation="MPKAAAGVLTVSTSQDFAAKWLVHRLARFAAANPGIDLRVSATI
                     HNVDFAREDVDVAVRHGDGHWPGLDATRLCAEQIFAVCSPKLLAGRNRLRRPADVLKW
                     PLLRLEDQSKAWEQWFTLAEVAAPERLPGPVLNRASMLIDAAIDGQGIALARTTLAAW
                     DLISGRIVRPFDLSWRPAGTYWIVCPKPAASIPKIAAFRQWLLAEAETDARRLAGATG
                     "
     gene            complement(1010..1702)
                     /locus_tag="VM910_10265"
     CDS             complement(1010..1702)
                     /locus_tag="VM910_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI62951.1"
                     /translation="MTDITVFGFPASSFVHIVRLVLTHKEVPYTFHDLEPDMGSPKHL
                     ALHPFDRVPILQHTDFMLYETSAIVAYLEEMFPRPALQPAQPRDRALMNQWISAVNSY
                     HYPYMIYHVGHERNVFPKLGIEPDEKVVAHALPKIEIGLQVVERQLADGRPYLVGADP
                     TIADYYLLPSTFSFARTDEGKAMYSGYPVFSAWRERMEALPTVRRFRAAESPYGPIEH
                     ARKWAVSHRPKY"
     gene            complement(1793..2032)
                     /locus_tag="VM910_10270"
     CDS             complement(1793..2032)
                     /locus_tag="VM910_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62952.1"
                     /translation="MSILSLPGARVSIRRSDSEPRATAWAVAARRTVTGWIARSRQRR
                     ALREIAENNDFHLLKDIGVSQEEAFREAEKPFWRR"
BASE COUNT          364 a          640 c          684 g          370 t
ORIGIN      
        1 cgccggtctg gcacaatgcc cgccagcaat ccggctcaaa tcccagttgc ttggttcgat
       61 cgaagggacc gttgcttcgt cccgacctgc gtccaccaac gaccgtagcg acgggtcggg
      121 gcaaggatcg ccaacggcga cggcggcagc cggcgcgcag cgtccttgcg gcggcccgcc
      181 gctgcggcca cactcgctca aggaggaacc cttgcctgat cgactgaaca accttcttgg
      241 aaacgacagc tagcgaaacc aatgaaatag gtgccgaaag ccgcggccgg cgtgctcaca
      301 gtcagcacct cgcaggattt cgccgcgaaa tggctggtgc atcgactggc tcggttcgcc
      361 gcggcgaatc caggcatcga cctgcgggtt tcggcgacga tacacaacgt cgatttcgcc
      421 cgcgaggacg tcgatgtcgc ggtgaggcac ggcgacggcc attggccggg tctcgacgcg
      481 acgcggctgt gcgcggagca gatcttcgca gtctgcagcc cgaagctgct tgcggggcgc
      541 aatcgcctga ggcggccggc cgacgtgctg aaatggccgc tgctacggct cgaggatcag
      601 agcaaggcat gggagcaatg gttcaccctc gccgaggtgg cagcgccgga gcgattgccg
      661 gggccggttc tgaaccgggc gagcatgttg atcgatgccg caatcgacgg ccaggggatc
      721 gccctcgctc gcaccactct tgcggcctgg gacctgatca gcggacggat cgtcagaccg
      781 ttcgacctgt catggcgtcc cgccggaacc tattggatcg tttgccccaa accggccgcg
      841 agcattccta agattgcagc tttccggcag tggttgctgg cggaagccga aacggatgcg
      901 cgccgcttgg caggagccac cggctagaga aggccttctc gctgaagagc gcagagcttg
      961 gaagggtttc ctctaacggg gagtctgcgt gtcccgatcg cgcgcaggat cagtatttcg
     1021 gccggtgcga aacggcccat ttgcgcgcgt gctcgatcgg cccatacggc gactccgccg
     1081 cccggaagcg gcgcaccgtg ggcaacgcct ccatgcgctc acgccaggcc gagaagactg
     1141 gatagcccga gtacatcgcc ttcccctcgt ccgtccgcgc gaacgaaaag gtgctgggca
     1201 acaggtaata gtcggcgatc gtcgggtcgg cgccgaccag gtagggtcgg ccgtcggcga
     1261 gctggcgctc gacgacttgc agtccgatct cgatcttggg cagcgcgtgc gccacgacct
     1321 tctcgtcggg ttcgatgccg agcttcggga acacgtttcg ctcgtggccg acgtggtaga
     1381 tcatgtacgg gtaatggtag ctgttcaccg cgctgatcca ttggttcatc aacgcccggt
     1441 cgcgcggctg cgcgggttgc agcgccggcc gcgggaacat ctcctcgaga taggcgacga
     1501 tcgcgctcgt ctcatacagc atgaagtcgg tgtgctggag gatcggcacg cggtcgaacg
     1561 gatgcagggc gaggtgcttg ggcgagccca tgtcgggttc gaggtcatgg aacgtgtacg
     1621 gcacttcctt gtgcgtcagc accagtcgta cgatgtgcac gaagctgctg gccggaaagc
     1681 caaaaaccgt gatgtcagtc atgggttttt ttctccattg cgcttcatcc tcgcgaagct
     1741 cactgaattt gcgcgaaaag cgcggagccc ttccgcacac gaggcccgag aattagcgcc
     1801 gccagaacgg tttttccgcc tcgcggaatg cctcttcctg cgagacgccg atgtccttca
     1861 gaaggtgaaa atcgttgttc tcggcgatct cgcgcagcgc gcggcgctgg cgtgagcgcg
     1921 cgatccagcc ggtgacagtg cggcgggcag caacggccca ggccgtggcg cgcggctcac
     1981 tgtcgctgcg ccggatggaa acgcgcgcgc cgggcaggct caatatcgac atttctggct
     2041 ccgtttatga gcttcgga
//
LOCUS       DATLJT010000444         1778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2553120,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000444 DATLJT010000000
VERSION     DATLJT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553120"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VM910_10275"
     CDS             complement(<1..607)
                     /locus_tag="VM910_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62953.1"
                     /translation="MRSKQRLRTYLGWLVATTVLIVAACFVVNCLVDPLWYLKGNVLT
                     GVNYPFNERLATVIRFLPRLKNYDCVIFGTSRGSLLPEERIEGYHCYNLSVSDGQASE
                     YLLYAKYLRERGFAPRLIIVDLKRAEFIGPQQAVEVPDFIRSGGAPPSILTSYMSLDA
                     LDFSIRTLRGDAPHHRYYDADFRAQLEVRSKRHRYDPKGPIE"
     gene            782..1369
                     /gene="pdxH"
                     /locus_tag="VM910_10280"
     CDS             782..1369
                     /gene="pdxH"
                     /locus_tag="VM910_10280"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HVI62954.1"
                     /translation="MDEIAEVEPLAPFQRWLDEAWRGEPNAHAMTLATATPDGRPSAR
                     MVLLKGLDARGFVFYTNLASRKSEELFANPHAALCVMWKSLNRQVRVEGPVERVDDEE
                     ADAYFASRPRDSQLGAWASDQSRPLESRATLEQRVDEFARRFAEGRVPRPKYWSGFRV
                     VPQRVEFWQERPFRLHDRLLFIREGQGWRRERLFP"
     gene            1366..>1778
                     /locus_tag="VM910_10285"
     CDS             1366..>1778
                     /locus_tag="VM910_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVI62955.1"
                     /translation="MIHGAPANRLRRLATYASVAVAAVLIAAKFAAWLETGSVALLSS
                     LVDSLLDIVASLVNLFAVRHAMSPADREHRFGHGKAEPLAVLGQSAFIIGSAMLLFAE
                     AIRRLIWPVPVENPPAGIAVMIFSIAVTVGLVLYQR"
BASE COUNT          330 a          541 c          557 g          350 t
ORIGIN      
        1 gctcgatcgg accttttgga tcgtaacgat gtcgctttga tctgacctcg agctgggcgc
       61 gaaagtccgc atcgtagtag cgatggtgtg gcgcgtcccc gcgcaacgtg cggatcgaga
      121 aatcgagcgc atcgagcgac atgtagctgg tcaagatcga cggaggcgcc ccgccgctgc
      181 gaatgaaatc cggcacttca acagcctgct gcggcccgat gaactcggcg cgtttgagat
      241 cgacgatgat caggcgggga gcaaaaccgc gttcccgcaa atacttcgcg tagagcaggt
      301 attcgctcgc ctggccatcc gaaaccgata aattatagca gtggtacccc tcgatccttt
      361 cttcgggcag caggctcccg cgcgaagtcc cgaaaatgac gcaatcgtaa ttcttgagcc
      421 gcggcaagaa ccggatgacc gttgccaggc gctcgttgaa cggataattc acgcccgtca
      481 ggacattccc cttcagatac cagagcggat cgaccagaca attgacgaca aagcacgccg
      541 cgacaatcag aacggtggtg gccaccagcc agccgagata ggtgcggagt cgctgctttg
      601 atctcacctt taatctcacc atggatactt ttccctccct gcacaaggcg cgcgatgccg
      661 ctgcagcggt catttccgcg cctcttcgtt tctttacaga ggctgtttcg ccgactgacg
      721 agtaccacat cgatgatacg cgcgctatgg tgggtcggac attcggccga cggaagcgtt
      781 gatggacgag attgccgagg ttgaaccgct ggcacccttt caaagatggc tcgacgaggc
      841 ctggaggggc gaacccaacg cgcatgccat gaccttggcg acggcgacac ccgatggccg
      901 gccgtcggcg cggatggtac tgttgaaagg gctcgatgcg cgcgggttcg tcttctacac
      961 caatctcgcg agccgcaagt cggaggagct gtttgcaaat cctcatgccg cgctgtgcgt
     1021 tatgtggaag tcgctgaacc ggcaggttcg cgtcgagggt cccgtcgaac gggtcgacga
     1081 cgaagaggcg gacgcctatt tcgctagccg gccgcgcgac agccagcttg gcgcctgggc
     1141 ctcggaccag tcgcggccac tcgaaagccg tgcgacactg gaacaacggg tcgacgagtt
     1201 tgcgcgccgc ttcgccgagg gtagagtacc gcgtccaaaa tattggtcgg gcttccgggt
     1261 cgtgccgcaa cgcgtcgaat tctggcaaga gcggccgttt cggctgcatg atcggttgtt
     1321 gtttattcgc gaaggccagg gatggcgacg ggaacggctt tttccatgat ccacggggcg
     1381 cccgccaacc ggctccggcg gttggcgacc tacgcctcgg tcgcagtcgc ggcggtgctg
     1441 atcgcggcca aatttgcggc ctggctcgaa accggatccg tcgcgctgtt gtcgagcctc
     1501 gtcgactcgc tgctcgacat cgttgcctcg ctcgtcaatc tcttcgcggt ccggcatgcg
     1561 atgagcccgg ccgaccgcga gcatcgcttt ggccacggca aggccgagcc gctcgccgtg
     1621 ctcggccaat cggccttcat catcggcagc gcgatgctgc tgtttgccga ggcgatacgg
     1681 cggttgatct ggccagtccc ggtcgagaac ccgccagccg ggattgcggt gatgatcttc
     1741 tcgatcgcag tgacggtcgg gctcgtcctc tatcagcg
//
LOCUS       DATLJT010000445         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4390547,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000445 DATLJT010000000
VERSION     DATLJT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390547"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VM910_10290"
     CDS             <1..922
                     /locus_tag="VM910_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI62956.1"
                     /translation="SVLVLDCRAFGGQAGASARIENYLGFPTGITGMALMARAYNQAQ
                     KFGVEMAIPDEVTALDAEDDVVSQRIVLRLANNEHVRARSLVIASGARYRRLSIPNLE
                     AFEGASVHYWASPLEAKLCAGQEVALVGAGNSAGQAAVYLANQAAKVWLLVRGDALAA
                     SMSRYLVDRIRGLPNVEVLTRTSVTGLEGHEGILEVIRWHRGATGEEVRRSIQHLFLF
                     IGAEPNTDWLSGSGVALDAKGFVLTGADTTNKLRPLETSREGVFAIGDVRSGSVKRVA
                     AAVGEGAQVVATLHPYLATAERNPAVSRAE"
     gene            1808..>2974
                     /locus_tag="VM910_10295"
     CDS             1808..>2974
                     /locus_tag="VM910_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HVI62957.1"
                     /translation="MADRFLREVQRMHQLRQDINEMTPEEADELIRGLGDDAAEDWVF
                     AARDAQLPPPDLSWCWLFLGGRGAGKSHSMSAAVHPAVRAGIKRIHFIAPTTTDFHDV
                     NVEGKSGIFATCGRDPRPRWVSSRRRLEWPNGAMCVFFSGEEPESLRGPQCELCVIDE
                     IARMHYQQAVFDTMMLGLRLGDRPRVLIGTTPRTTPFMKRLVAMDDIRITSGSTYDNA
                     DHLSPAFLNKVRELYEGTRLGRQELQGAMILDPANALFKDDWLVHDEVAEDLIEQVTV
                     GVDPSGGGDEVGIVASALLNDGRFAVLADRTTSGSPAVWGEAVVRCHDDFDADDVVVE
                     VNFGGDMATEVVKQAAERVHQRGDRETNLIRIKEVSASRGKVMRAEPISLLYEKG"
BASE COUNT          605 a          820 c          976 g          573 t
ORIGIN      
        1 gtccgttctt gtgctcgatt gccgcgcatt cggcggccag gcgggcgcct cggcacggat
       61 cgagaactac ctagggtttc caacgggcat cacgggaatg gcgctgatgg cgcgcgcgta
      121 caatcaggcg caaaaattcg gagtcgagat ggcgatccca gacgaggtga cagccctcga
      181 tgctgaggat gacgtggttt cgcaacgcat cgtcctaaga ctggccaaca atgaacatgt
      241 gcgggcgcgt tcgctcgtca tcgcaagcgg agctcgttac cggcggctat caattcccaa
      301 cttggaagca ttcgagggcg caagcgtaca ctattgggcc tcgccccttg aagcgaagct
      361 gtgcgctgga caagaggtgg cgttagtcgg cgccgggaat tcggctgggc aggcagcagt
      421 ctacttggcg aaccaggcag ccaaggtttg gctcttggta cgcggcgatg ctctcgccgc
      481 gagcatgtcg cgctacctcg tggatcgtat tcgagggctt cccaacgtcg aagtgctgac
      541 gcggactagc gtcaccggcc tcgaaggcca tgagggaatc cttgaagtga ttcgctggca
      601 tcgcggagcg acgggggaag aagttcggcg gtcgatccaa catctgttcc tcttcatcgg
      661 tgctgagccg aatacggatt ggctttcggg atcaggcgtt gctctggacg caaaggggtt
      721 cgtcttgacc ggcgcggaca cgaccaacaa gctgcggccg ctcgagacca gccgagaagg
      781 cgtgtttgct atcggtgacg tgcgctcggg ttcggtcaag cgagttgcgg ccgcggtggg
      841 cgagggcgcg caagtggtgg ctacgctgca tccatatttg gccacggccg agcgcaaccc
      901 ggccgtctca cgagcggagt aggtcgcaat agatcaggct ggaggcgaac acgccgcgca
      961 gtccggggta ggaggtgcgg atcgtcggcg cagtatttgg cgaaagcatt cgtgacgtct
     1021 gctaacgagg cacacacacc gttttggcga aagcgtccgt ctcagctcaa atttggccaa
     1081 cccgttgagc aggaatcaaa attagcgggc ggatggtgac ggatgcgtca cctgatgcca
     1141 ggtgtcgcgg cggcgggctc agggcgttac tcggggagag atcagcaccc acgagcatcg
     1201 ttgcggcatc gatctctgtt caccggcagt aattggccgc cgcagtcagt gtgcgctcgt
     1261 gtcggtcgat cgcgctaccg ttgcgcccgg gagaaatgcc gacccccacc ccacgatgga
     1321 caggcccctc tctaaaccgc ggcactgcca tgaactcgcc tcagattttg tctcgcgttt
     1381 tggcgaaaac atccgtctca gctcatattt ggctaacccg ttgagcagga atcaaaatta
     1441 acgggcagat agtgacggat gcgtcgggcc atatttgaac taaccgttga gcaggaatca
     1501 aaattggcgg gcggatggtg acggatgcgt cgttcatatg tgagctaacc cgttgagcaa
     1561 gagccgacat tggcgggcgg atcgcgaaag aaatcgttac cgcttgtgtc gccctcggca
     1621 aaacgatggt cgatcgtgat attttgcccc tcgatgaggc cattctcgat caagccggcc
     1681 ttaaactgct ccatcaggtg cggccacgcg tttctgacga ccgagaaaat ctgcgcgctc
     1741 gtcgataata taagaggcaa gaaagatcgc gacatcgaag gcgaagtcgt gaaaaaggac
     1801 gacgacgatg gctgatcgct ttttgcgtga agttcagcgc atgcaccagt tgcgccaaga
     1861 catcaatgag atgacaccgg aggaagccga tgagctgata cgaggactgg gcgatgacgc
     1921 ggccgaggac tgggtctttg ccgcgcgcga tgcacaactg ccgccgcccg acctgtcatg
     1981 gtgctggctg tttctcggcg gccgcggcgc cggcaaatca cactcaatgt cggcggcggt
     2041 gcatcccgcg gtgcgcgctg ggataaagcg cattcacttc atcgccccaa ccacgaccga
     2101 ctttcacgat gtcaacgtcg aaggcaagag cggtatattt gcgacctgcg gccgtgatcc
     2161 acggccgcgc tgggtgtcat cgcggcgacg gctggaatgg ccaaacggcg caatgtgcgt
     2221 gtttttctcc ggcgaagagc cggaatcgtt gcgcggcccg caatgcgaat tgtgcgtgat
     2281 cgacgagatc gcgcgcatgc attaccagca ggcggtgttc gacaccatga tgctgggctt
     2341 gcgattgggc gatcgtccgc gcgttctgat cgggaccacg ccaaggacga ccccgttcat
     2401 gaaacggctg gtggcgatgg acgatatccg gattacgagc ggctcgacct acgacaatgc
     2461 cgatcatctg tcgccagcgt ttctgaacaa ggtcagggag ctttacgaag gcacgcggct
     2521 cggccggcaa gagctacaag gcgcaatgat tctcgatccg gcaaatgcgc tgttcaagga
     2581 cgattggctt gttcacgatg aggtggcgga ggacttgatc gagcaggtaa ccgtcggcgt
     2641 tgatccatcc ggtggcggcg atgaggtcgg catcgtggct tccgcgctgc tcaacgacgg
     2701 ccggttcgca gtgctggccg atcgcaccac cagcggcagc ccggcggtgt ggggcgaagc
     2761 ggtggtgcgc tgccatgacg atttcgatgc cgacgacgtg gtggtcgagg tcaatttcgg
     2821 cggcgacatg gcaacagagg tcgtgaagca ggctgccgag cgtgtgcatc agcgcggcga
     2881 tcgcgagacg aacctgatcc gcatcaagga agtgtcggct tcgcgcggca aggtgatgcg
     2941 ggccgaaccg atctcgctgc tctatgaaaa gggt
//
LOCUS       DATLJT010000446         1890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2322959,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000446 DATLJT010000000
VERSION     DATLJT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322959"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VM910_10300"
     CDS             <1..235
                     /locus_tag="VM910_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HVI62958.1"
                     /translation="IAVTTQGRNRQERLQIDQRQDGRGNAYYWIAYVRMRAQPPADGS
                     DISALADNRIAVTPLRLDMTDEPFMTRLAELFD"
     gene            complement(299..691)
                     /locus_tag="VM910_10305"
     CDS             complement(299..691)
                     /locus_tag="VM910_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI62959.1"
                     /translation="MASVAQAQPAMPAKRILVVEDELMIRMLLEDMLGELGYTVTAEA
                     GRLDEALQAAKTADFDLAILDVNLDGEPVSPVADALVARGTPFVFATGYGERGLPEPY
                     RDRPMLKKPFQMDGLKEMLQSALEGGKN"
     gene            905..1525
                     /locus_tag="VM910_10310"
     CDS             905..1525
                     /locus_tag="VM910_10310"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVI62960.1"
                     /translation="MQFLLGLRKRGISDAAVLRAMDEVPREHFVESSFMDNAYADQAL
                     PIACGQTISQPYVVAFMTEQLGVRPEHRVLEVGTGSGYQAAILSRLAREVISVERYRT
                     LADTARQRLATLGYGNVDVRVGDGLAGVPDGAPYDRIMVTAAAETIPEALVGELADGG
                     VMVLPLGPHAGPQELVKLTKGEQGIKQEDLIGVRFVPLLPGKAREL"
     gene            complement(1595..>1890)
                     /locus_tag="VM910_10315"
     CDS             complement(1595..>1890)
                     /locus_tag="VM910_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62961.1"
                     /translation="RSTDPVTSEAAPEFAATDVAECDCGACAVCACGIPAVGAGLLRE
                     ANGLSLNRVVSALQAATLTLTSASTTARGENRERNICETPDITPYSHATQAAS"
BASE COUNT          322 a          585 c          630 g          353 t
ORIGIN      
        1 cattgccgtg accacccagg gccgcaaccg gcaggagcgg ctgcagatcg accagcggca
       61 ggacggccgc ggcaacgcct attactggat cgcctatgtg cgcatgcggg cgcagccgcc
      121 ggcggacggc agcgatattt ccgcgctcgc cgacaatcgc atcgcggtca cgccgctgcg
      181 gctcgacatg acggacgagc cgttcatgac acggctggcg gaactcttcg attaaggtgg
      241 cgtctcgggc tttgaaaact gtgtacgcgc tcgcgccgca tccggaacac gcggggcttc
      301 aattcttgcc gccctccagc gcgctttgca gcatttcttt gagcccatcc atctgaaacg
      361 gcttcttgag catcggccgg tcgcgatagg gttcgggcag gccgcgttcg ccgtaacccg
      421 tggcaaacac gaagggcgtg ccgcgggcga cgagggcatc ggccaccggc gagacgggct
      481 cgccgtcgag attgacgtcg aggatcgcga gatcgaagtc ggcggtcttc gccgcttgca
      541 gcgcctcgtc gagacgccca gcctcggcgg tgacggtgta accgagctcg ccgagcatgt
      601 cctcgagcag catgcggatc atcagctcgt cttcgacgac gaggatccgc tttgccggca
      661 tcgccggttg ggcttgagcg acagaggcca tctttcttcc ccgttgcgat cgaatgcgca
      721 accaggagga caccagatcg tcaaggcgaa gttgaggcgc cgcttccggt tgcttgttcc
      781 gccgcaaact attggattta cgtcgttttc accgtgcgct ctggccgaag ggggttttcg
      841 gctagagtcg gccaggaggc tcggcgtgcc caagcaaccg cccggcgacg acagtgtcgg
      901 ccgcatgcag tttctgctcg gcctgcgcaa gcgcggcatc agcgatgcgg cggtgctgcg
      961 cgccatggac gaggtgccgc gcgagcattt cgtcgaaagt tccttcatgg acaatgccta
     1021 cgcggaccag gcgctgccga tcgcctgcgg gcagacgatc agccagcctt acgtggtcgc
     1081 cttcatgacc gagcagctcg gcgtgcggcc tgagcatcgt gtgctcgaag tgggcaccgg
     1141 ctcgggctat caggccgcca ttctgtcccg gctcgcgcgt gaagtgatca gcgtcgagcg
     1201 ttatcggacg ttggcggaca ccgcgcgtca gcgactggcg acgctcggct atggcaatgt
     1261 cgacgtgcgg gtcggcgatg gtctcgccgg cgtccccgac ggagcgccct acgaccgcat
     1321 catggtcact gccgccgccg aaaccattcc cgaggcactt gtgggcgagc ttgccgacgg
     1381 cggcgtgatg gtattgccgc tcggaccgca cgccggtccg caggagctgg tcaaactcac
     1441 aaagggtgaa caaggaatta agcaggagga tttgatcggg gtacgattcg tcccgctttt
     1501 gcctggaaag gcgcgcgaat tgtaacgatt gtgttcacgc gacggcaagg ttgaggcgcc
     1561 gtaatctctt aaagtcgtat taacccgcaa ggtgttaact cgccgcttgg gttgcgtgcg
     1621 agtagggtgt gatgtcgggg gtttcgcaga tgttgcgctc gcggttctca ccgcgggcag
     1681 tggtgctggc gctggtgagc gtcagcgttg ccgcctgtag cgcggatacg actcgattca
     1741 acgacaatcc gtttgcctcc ctcagcaatc ccgcgccgac cgcagggatg ccgcaggcgc
     1801 aaaccgcgca ggcgccgcag tcgcattcgg cgacgtccgt agcagcgaat tccggcgcgg
     1861 cttccgaagt cacgggatca gtgctgcgca
//
LOCUS       DATLJT010000447         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1404159,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000447 DATLJT010000000
VERSION     DATLJT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404159"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1340)
                     /locus_tag="VM910_10320"
     CDS             complement(108..1340)
                     /locus_tag="VM910_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI62962.1"
                     /translation="MNGHAQAKAAEPLFNPLSPDFIRDPYPHYERLRTSDPMHVTAHG
                     AFLASRHAEVSLVLRDKRFGKDFVDRTIRRYGPKIMEEPIFRSMSHWMLQQDQPDHTR
                     LRGLVVKAFTARRVEDMRPRIQEVVDRTIDDVIARGHMDLIEDFAFRLPVTIICDMLG
                     IPEEHREVFYKSSRDGGRILDPVPLTPAEIAEANMGHAMAKMYFEQLFELRRRNPGDD
                     LTTQLVQAEEDGSRLTNEELIANIILLFGAGHETTVNLIGNGLLALHRNPDQLALLKA
                     NPSLISNAIEEFLRYDSSVQMTGRVALEEIEDLGGKRIPKGESVLCLLGSANRDPAVY
                     SDGPDRLDITRPNVKPLSFGGGIHFCLGAQLARIEAEIAISTLLRRIPDLRLDDAENP
                     QWRPTFVLRGLKQLPSSW"
     gene            1429..2040
                     /locus_tag="VM910_10325"
     CDS             1429..2040
                     /locus_tag="VM910_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI62963.1"
                     /translation="MSRVRTRPTRDETRDKLFEAAARVFEEQGIGGASIEAIAAAAGF
                     TRGAFYSNFASKDELIIAMLEDHVAQSIRRSLDLLAKHQNLDDFLDAFKRMDRSQQDP
                     LGRSPLLHMEMILFVARAEKRRPELAKRLRARRKLIADIVETTLKDSGRPASVNPAWT
                     AAVVLALDDGFRLHRLIDPETTPADSFLRALSDLRRSTGLSSA"
     gene            complement(2077..3393)
                     /locus_tag="VM910_10330"
     CDS             complement(2077..3393)
                     /locus_tag="VM910_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62964.1"
                     /translation="MKETLPHRLPFFYGWVVVAVTFVTMGIGVNARTAFSLFYPPILS
                     EFGWERGVTAGAFSFGFVVSAVISPLIGRLMDRAGPRAVMELGVALMAGGLLLAPLTR
                     EPWHLYLTIGVMVGAGSVCLGYSGFSLFLPNWFIRRRGLAIGIAFAGVGIGSVTLLPW
                     AQHVIEVSGWRTACTAIGLMVLIVLAPINLLLRKRPEDIGLLPDGDAAPHALAAKPVS
                     NILDPAWAATDWTLPRALRTARFWWIALGYFCFLYIWYAVQVHQTKYLLDIGFSPNVG
                     VWALGVVSLLGIPGQIVLGHASDRIGREAIWAISSAGFAICFASLIALKYEPSLLLVY
                     LMVFTQGALGYGLTSIMGAVVLEIFQGAHYGSIFGCIMLVALAGGAAGPWVTGLLYDW
                     TGSYTIAFAVAIAVSALSALAIWRASPGKVRAVAGKLRHVQAEGGG"
     gene            complement(3390..>3750)
                     /locus_tag="VM910_10335"
     CDS             complement(3390..>3750)
                     /locus_tag="VM910_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI62965.1"
                     /translation="IANEETENLIGQATLARMQKHAFFINLSRGNLVDEAALAAALRE
                     NLIAGAAMDVGRASDQMPTPKLAKLPNVIATPHIGGLTPQAIEHQAMETVGQVASIIK
                     GEVPIGAVNAERWTRRR"
BASE COUNT          715 a         1239 c         1186 g          610 t
ORIGIN      
        1 gacgctaatg tcccgaatcc gcggcccacc cgccgccgcg tcaccggagc gcccaatata
       61 gggagaagta cggcggagtc acagcgcctt cgtgatcacg accgctttta ccagcttgac
      121 ggcaactgct tcagcccgcg cagcacgaag gtcggccgcc actgcggatt ttccgcgtca
      181 tccagccgca ggtccgggat gcggcgcagc aacgtgctga tcgcgatctc cgcctcgatt
      241 cgcgcgagct gggcgccgag gcagaagtgg attccgccgc cgaaggacag cggtttgacg
      301 ttggggcgcg tgatgtcgag ccggtccggc ccgtcggaat agacggcggg atcgcggttg
      361 gcggagccca gcaggcatag cacgctctcg cccttcggaa tccgcttgcc gccgagatcc
      421 tcgatctcct ccagcgccac gcgcccggtc atctgcaccg aagaatcata gcgcaggaat
      481 tcctcgatcg cgttgctgat cagcgacgga ttggctttca gcagcgcgag ctggtcgggg
      541 ttgcggtgca gcgccaacag cccgttaccg atcagattga ccgtcgtctc gtggccggcg
      601 ccgaacagca gaatgatgtt ggcgatcaat tcctcgttgg tcagcctgct gccgtcctcc
      661 tccgcctgca ccagttgcgt ggtcaggtcg tcacccggat tgcgccggcg caactcgaag
      721 agctgctcga aatacatttt cgccatcgcg tggcccatat tggcctccgc gatctcggcc
      781 ggcgtcaacg gcaccggatc gaggatccgc ccgccgtcgc gcgagctctt gtaaaacacc
      841 tcgcggtgct cctcggggat gccgagcatg tcgcagatga tggtgaccgg caggcgaaag
      901 gcgaaatcct cgatcaggtc catgtggccg cgcgcgatga cgtcgtcgat cgtgcgatcg
      961 accacctcct gaatccgcgg ccgcatgtcc tcgaccctgc gcgcggtgaa cgccttgacc
     1021 acgagcccgc gcaggcgggt gtggtccggc tggtcctgct gcagcatcca gtggctcatg
     1081 ctgcggaaga tcggctcctc catgatcttg gggccgtaac ggcggatcgt gcggtcgacg
     1141 aaatccttgc cgaagcgctt gtcgcgcagc acgaggctga cttcggcatg ccggcttgcg
     1201 aggaacgcgc catgcgcggt gacgtgcatc gggtcggagg tgcgcagccg ctcgtaatgc
     1261 ggatagggat cacggatgaa gtccggcgag agcggattga acagcggttc ggctgctttg
     1321 gcctgcgcat gcccgttcat gagacctcgc tcttattcct attggcaaaa attgtctgca
     1381 actcgataca ttgttgtatc gagttgcatt ctgccctaac ctgtgacgat gtcaagggtg
     1441 agaaccagac cgacgcggga cgagacccgc gacaaactgt tcgaagccgc cgcgcgggtg
     1501 ttcgaggaac aggggatcgg cggcgccagc atcgaagcga ttgcggccgc cgccggcttt
     1561 acccgcggcg cgttctattc gaactttgcg agcaaggacg aattgatcat cgcgatgctc
     1621 gaagaccacg ttgcgcagtc gatccggcgc agtctcgatc tgctggcaaa gcatcaaaac
     1681 ctcgacgact ttctcgatgc atttaagagg atggaccgca gccagcagga tccgctcggc
     1741 cgctctcccc tgctgcacat ggaaatgatc ctgttcgtgg cgcgcgcgga aaagcgcagg
     1801 ccggaacttg ccaagcgcct gcgcgcacgg cgcaagctca tcgccgatat cgttgagacc
     1861 acgctcaaag actccggcag gcccgcctcg gtcaatccgg catggaccgc ggccgtcgtg
     1921 ctcgcgctgg atgacggttt ccgcctgcac cgcctgatcg atccggagac cacgccggcc
     1981 gacagcttcc tgcgcgcgct ctccgatctg cgacgctcaa ccggactgtc atcggcgtga
     2041 cgggggcgtg acaaaccctg atcgcaacgt gccctgtcaa ccgccccctt ccgcctgaac
     2101 gtgccgcagc ttgccggcca ccgcacggac cttgcccggc gaggcgcgcc agatcgcaag
     2161 cgccgacagc gcgctcacgg caatcgcaac cgcgaatgcg atcgtgtagc tgccggtcca
     2221 gtcgtagagc aagccggtca cccagggacc ggcggcgccg cctgcgagcg ccacgagcat
     2281 gatgcagcca aagatgctgc cgtaatgcgc gccctgaaaa atctccaaca ccaccgcgcc
     2341 catgatcgag gtgaggccgt agccaagcgc accttgcgtg aacaccatga gatagaccag
     2401 cagcaggctc ggctcatatt tcagagcgat cagtgacgca aaacagatcg caaagccggc
     2461 ggagctgatc gcccagatcg cctcgcgccc gatccggtcg gatgcatggc ccagcacgat
     2521 ctggccgggg atgccgagca ggctgacgac gccgagcgcc cagacgccga cattcggcga
     2581 gaagccgata tcgagcagat atttggtctg gtgcacctgc acggcgtacc agatgtaaag
     2641 gaagcagaaa taaccgagcg cgatccacca gaagcgcgcg gtccgcaatg cgcgcggcag
     2701 cgtccagtcg gtcgccgccc aggcgggatc gagaatattg gagaccggct ttgcggcaag
     2761 ggcgtgcggc gcggcatcgc cgtccggcaa gaggccgatg tcctcgggac gcttgcgcag
     2821 caacagattg atcggcgcga gcacgatcag caccatgagg ccgatcgccg tgcaggcggt
     2881 gcgccagccg ctcacctcga tcacatgctg cgcccagggc aacagcgtca ccgagccgat
     2941 cccgacgccg gcgaaggcga tgccgatcgc aaggccgcgg cggcgaatga accagttcgg
     3001 cagaaacagc gaaaagccgg aatagccgag gcacacgctg cccgccccga ccatcacgcc
     3061 gatcgtgaga tagagatgcc agggctcacg cgtcagcggc gcgagcagga gcccgcccgc
     3121 catcagcgcg accccgagtt ccatcaccgc gcgcgggccg gcgcgatcca tcaggcggcc
     3181 gatcaacggg ctgatcacag ccgacaccac aaagccgaac gaaaaggcgc ccgcggtgac
     3241 gccgcgctcc cagccgaact cggagagtat cggcgggtag aacagcgaga acgcggtgcg
     3301 cgcgttgacg ccgatcccca tggtgacgaa ggtcacggcg accacgaccc agccgtaaaa
     3361 gaagggcagc cgatgcggca atgtctcttt catcgccgac gcgtccagcg ctcggcattg
     3421 accgcgccga tcggcacctc gcccttgatg atggaggcga cctggcccac cgtctccatc
     3481 gcctggtgct cgatcgcctg cggcgtcagc ccgccgatgt gcggggtcgc aatcacgttg
     3541 ggaagctttg caagcttcgg cgtcggcatc tggtccgacg cacggccgac atccatcgcg
     3601 gcccccgcga tgaggttctc gcgcaaggct gcggccagcg ccgcttcgtc gacgagattg
     3661 ccgcgcgaca ggttgatgaa gaaggcatgc ttctgcatcc gcgccaatgt cgcctgcccg
     3721 atcaggtttt cggtttcttc attggcgatc
//
LOCUS       DATLJT010000448         2660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_255717,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000448 DATLJT010000000
VERSION     DATLJT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255717"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1338
                     /locus_tag="VM910_10340"
     CDS             346..1338
                     /locus_tag="VM910_10340"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI62966.1"
                     /translation="MQFDQMKRREFITLLGGTAVWPVGARAQQPAKLPTIGFLGSGTP
                     STARQWVAAFVGRLRELGWVEGRTVAIDYRWAEGREERYAEFATDFARLKADLIFTAG
                     TSAVLAAKQATSIIPIVFAAAGDPVGNGLVTSLAHPGGNVTGLSLQQPDLGAKWLELL
                     REVVPNLARLAIMANIGTPGAVSEMKEVEAVARALGLEPVTLEIGRAEDIARAFETLQ
                     GRASALYICSDPLLNAHRMRINTFALVARLPTMNLFREYVESGGLMSYGPNLADLYRR
                     SGDYVDKILRGTKPADMPIEQPTKFDLIINLTTAKAPGLIVPDKLLAVADEVIE"
     gene            complement(1733..>2660)
                     /locus_tag="VM910_10345"
     CDS             complement(1733..>2660)
                     /locus_tag="VM910_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI62967.1"
                     /translation="VVYTLPAELRDIAYQNKAVVYDLLFKASAETTLTIAADPKHLGA
                     RIGFISVLHTWGSALVHHPHVHMIVPGGGFSSDGSKWIACRPRYFLTVEVLSALFRRL
                     FLEMLCAAHKAGQLLFFGNLVPLAEKAAFEAYLKPLCNIDWVVYAKEPFAGPRQVLRY
                     LSCYTHRIAISNRRLISADENGVTFRYKDYRLDRPARYKTMTLTTDEFIRRFLLHVLP
                     KRFHRIRHYGLLASGNRAVNLAHARELLAVPPEQPQPAKAEESDAQSVLPRPCPCCGG
                     RMIIIETFARGCEPKLRPTSAPAPQQIRIDTS"
BASE COUNT          510 a          743 c          902 g          505 t
ORIGIN      
        1 ccgccacgtt ggtcggctac gacactctca atgccgccgg ggagcggctg ctggccgatg
       61 agatattcaa tacggtgctg gtgctactgg tcgttacatc acttcttggg ccgatcctga
      121 cggagctgtt cgcgcctggg atggtgaggc agcaggcgcg cacgacgccg gcgacctgag
      181 gggctggtcc gacctggaaa cggaaagcgg agatgtccgc gatcagggag agtcttggag
      241 ggtaagcgga ctaccggcaa gctcggtcgt gccgcgctat gtgccactga ctgaccgctg
      301 ctaactcgtt atgatccgtt ttacagtcgg ctggcagcgg accgaatgca attcgatcag
      361 atgaagcgtc gcgagttcat cacgctgctc ggcggaacgg cggtatggcc ggtcggggcg
      421 cgtgcgcaac agcctgcaaa actgccgacc attggctttc tgggctcagg aacgccttcg
      481 accgcgagac aatgggtcgc cgcttttgtg gggcggttgc gcgagctcgg ctgggtcgag
      541 ggtcgcaccg tcgccatcga ctatcgatgg gcggaggggc gcgaggagcg atatgccgag
      601 ttcgccaccg acttcgctcg gctcaaggcc gatctcattt tcacagcggg aacctcggcg
      661 gtccttgccg cgaagcaggc aacctcgatc atcccgatcg tgtttgcggc cgcgggagat
      721 ccagtcggca atggcctcgt aacaagcctt gcgcatccgg gcggcaacgt caccggcctg
      781 tcgctccagc agcccgatct cggcgccaaa tggctcgaac ttttgcgtga ggttgtgccc
      841 aatctcgccc ggctggccat catggccaat atcggcacac ccggcgccgt atcggaaatg
      901 aaagaggttg aggctgtggc ccgcgcgctg ggccttgaac ccgtcacgtt ggaaatcgga
      961 cgcgcggagg atatcgcgcg cgcgttcgag acgctccaag gccgcgcgag cgcgctttac
     1021 atctgtagcg acccgctcct gaacgcccac cgaatgcgca tcaacacttt cgcgctcgtt
     1081 gcgcgtctgc cgacgatgaa tctttttcgc gagtacgtcg aatctggcgg tctaatgtcg
     1141 tatggcccga acttggctga tctgtaccgg cgatccggcg actatgtcga caagattctg
     1201 cgcggaacga agcctgccga tatgccgatc gagcagccga caaagttcga tctcatcata
     1261 aatctgacca cggcaaaggc gcccggcctg atcgtgccag acaagctgct tgcggtggcg
     1321 gacgaggtga tcgaataggc aggcatttgc tgcacctgct cacctccggt cctgtgcagg
     1381 tttttggctg ccggcaacga tgatctgacc acgcgctctg gcggccggcg gccaaaaagc
     1441 gccaaagcac gaaatcgcgg tgacgggtag tgcggcggga gtgccgcagg ctatggggat
     1501 ttagccgtgg cgtgagagag taggacggtt gcgcggtgcg gccgcagcag ttggtttgtt
     1561 gcgaatgtga cgggaagacg cgagggaacg gcagctggcc gcacggttgg cgacaagatt
     1621 ggtgcgccac gcgggctcga atcaagggca acatcacgag cggcagcgtt actggatgca
     1681 aaccagcgag acggatgcct tccgctgcag tcattgaccg gttgtgacgg cgtcatgagg
     1741 tatcgatcct gatctgttgc ggcgcgggtg cggaagtggg gcggagcttg ggttcacaac
     1801 cgcgggcgaa ggtctcgatg atgatcatgc gtccgccgca gcaggggcac ggacgcggca
     1861 atacggactg cgcatccgat tcttcggctt tggcgggctg gggttgctct ggcggcacgg
     1921 cgagcagctc acgcgcatgc gcgagattta cggctcggtt gccgctagcg agcagtccgt
     1981 agtgtcgaat gcggtggaag cgcttgggca ggacgtggag caggaagcgg cggatgaact
     2041 catctgtggt gagcgtcatc gtcttgtagc gggccggccg gtcgagccga taatccttgt
     2101 acctgaaggt gacaccgttc tcatcggcgg atatgagccg gcgattagag attgcgatgc
     2161 ggtgcgtgta gcacgagaga taacgcaaga cttgcctggg tccggcaaac ggctctttcg
     2221 cgtacacgac ccaatcgatg ttgcacagtg gttttagata ggcctcgaaa gcagcctttt
     2281 cggcgagggg gacaagattg ccgaagaaca gcagctgtcc ggccttgtgc gcggcgcaca
     2341 gcatctccag aaacaggcgc cggaagagcg cggagagaac ctcgaccgtc aagaaatagc
     2401 gcggtcggca tgcgatccat tttgacccgt ccgacgaaaa gccgccaccg ggcacgatca
     2461 tgtgcacatg tgggtggtgg acgagggcgg agccccaggt gtgcaatacc gagatgaagc
     2521 cgatgcgtgc gcccagatgc tttggatcgg ccgcgatcgt gagcgtggtc tcggcggagg
     2581 ccttgaacag cagatcgtag accactgctt tgttttggta tgcgatgtca cgcagttcgg
     2641 ccggcagcgt atagaccacg
//
LOCUS       DATLJT010000449         5059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_255977,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000449 DATLJT010000000
VERSION     DATLJT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255977"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VM910_10350"
     CDS             <1..662
                     /locus_tag="VM910_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HVI62968.1"
                     /translation="ARAKLTDSRERPNGDLRVTTTPGIGVHWLTPRLGEFADLYPEVH
                     ITLLTTDEELDLAMREADVAIRLRQPTQPDLIQRKLFSVHFHVYASPEYLKRFGTPRN
                     LEDLDNHRLLVLGGNVPPYLQRARWLIEVARDGKGPRIPHLVINNILGILRACQRGLG
                     IAMLPDYLVEENGGLVQLFGEGDAIALDAYFVYPEELKNVARVQAFRDFLVANAQRWN
                     F"
     gene            complement(917..1351)
                     /locus_tag="VM910_10355"
     CDS             complement(917..1351)
                     /locus_tag="VM910_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62969.1"
                     /translation="MRIIVTTCSALLLLGAHTRAEVLITASEAVQPPSSDVRINVRGI
                     TRGPSVDQILPSPTTSVHSPFELKIRFTAHNSAKVDPSDVRVIYEKQMPIDLTERLRK
                     YMSSEGIDMPDVEVPPGTYLLRIEVKDSLDRKSVGEVKLLVQ"
     gene            complement(1400..2221)
                     /locus_tag="VM910_10360"
     CDS             complement(1400..2221)
                     /locus_tag="VM910_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62970.1"
                     /translation="MKCPFCAEEVKDEAIVCKHCRHDLSVVRALMERIEDLTKQFRSV
                     ISDPAATREESITHAPIPQGAHQERVAVIAGAIERHIPLLSPLATVLLALGALLLAHF
                     IIVIHYDLSLFWLHIVSLVLPFVFGFIYRQNTTEYLSSDLAAGILLAAAAIFAMSAVV
                     AEVDRVPVLPMDRQGWLEYLEYGASIALAFFAGAVCRQTAIAMSTPTAKTSRFVYFAA
                     KYATAKVSGVSPQDPNDPRMDKYLKRVGLAEKIITASIAGVSALVSIWSGLGQFL"
     gene            complement(2255..4915)
                     /locus_tag="VM910_10365"
     CDS             complement(2255..4915)
                     /locus_tag="VM910_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HVI62971.1"
                     /translation="MMGDISQAGTYAGRVEALVQEARGSPNPSWRQSYSVYGHSWEAD
                     ADRARGLVFEARGQYQEAEAAYRRAEAFRRAGIKDLPRYDYPPPAEQMMLAADSHLLA
                     AARNESREGKLSEAEADARRALLGVLKAQGKYTPTTPQFIVGLAAILVEQGRYQEAEK
                     LARSALDIQRTLGIRDDAPQSVAMLSGLGNILVAERKAKEAAIVYAQLDKAMEQWGPQ
                     QREAFELNGSRIAALYASGQFEAGIAGAEALVKRQIARTGANSFDAASAHGTLAEGYA
                     RAGRDADAIAEFKVAVPMLMSAARETDNNDPTLVAARNARLQRIVEAYIAVLARSQNA
                     SNDVAVETFGLADAVRGQAVQQALADSSARMVAKDAALAELVRTEQDRAKQISAELGT
                     LNNLLALPSDQRDSQTVHAIRTEIEQLRTERKTAQQQIKQRFPAYATLIDPKPPTVDE
                     IKAALRSGEALLSFYFGQDKSFVWAVPKEGAVAFAAVPATAVQLEANVRKLREALEPQ
                     VTMVSEIPAFDVALAYQLYATLLKPVETGWRGSKSLIVVTNGALGALPLGLLPTAPAQ
                     VDLAAKPLFAGYRNVPWLARTHAVTVVPSASALITLRHLPPGTSSRDKLIGFGDPYFN
                     AQEAADANAEQQVPMQVASAETSDDDAVTTRGIPLKLRAAPHIEDVDTAELALLPRLP
                     DTRLELHAVAKALDVDPAKSLYLGRDANEQNVETINLSHYQIIAFATHGLVPGDVDGL
                     TQPALALTAPEVAGVKGDGLLTMEKILALKLDADWVVLSACNTAAGAGAGAGAEAASG
                     LGSAFFYAGTRALLVTNWSVHSVSARELISDVFRRQSADSSISRSEALRQAMMALLDG
                     PGTVDRSGHSIFSYAHPLFWAPYSLIGDGG"
BASE COUNT          988 a         1557 c         1478 g         1036 t
ORIGIN      
        1 cggcgcgcgc caagctcacc gacagccgcg agcggccgaa cggcgatcta cgggtgacga
       61 ccacgccagg catcggcgtg cactggctca cgccgcggct cggcgagttt gccgaccttt
      121 atcccgaagt tcacatcacg cttctcacca ccgacgagga actcgacctg gcgatgcgcg
      181 aggccgacgt cgccatccgg ctccgccagc ccacccagcc ggatctcatt cagcgcaagc
      241 tgttttcggt gcatttccac gtctatgcct cgccggaata cctgaagcga ttcggcacgc
      301 cgcggaattt ggaggacctc gacaatcacc ggctcctagt gctcggcggc aatgttccgc
      361 cctatctgca acgcgcccgc tggctgatcg aggtggcgcg cgacggtaag gggccgcgga
      421 tcccgcacct ggtcatcaac aacattctcg gcatcctgcg cgcctgccag cgcgggctgg
      481 gcatcgcgat gctgccggac tacctggtcg aagagaatgg cgggctggtg caattgttcg
      541 gcgagggcga cgccatcgcg ctcgacgcct atttcgtcta tccggaagag ctgaagaacg
      601 tcgcccgcgt tcaagcgttc cgcgacttcc tggtcgccaa cgcgcagcgt tggaattttt
      661 agccgagcga ttagctaagg cgatccgctc gtgcggcgtt gtccgttgct gcctgctttg
      721 ccaccgcgct cgcgcaaacg aaccccctcg caaatttgta gagaattttg aaggtatggc
      781 tagttcactg cggacttgtt gttagaccaa agcgatgtcc gctttgcccc cgaaagcgga
      841 catcgctcag cacggttgtg atgtccgctt gtgccaaaag cggacatcga gttatcccaa
      901 agccgaaggc ggcgactcat tgaacgagca gcttgacctc gccaaccgac ttccgatcga
      961 gactgtcctt aacttcgatg cgaagcagat atgtacccgg tggcacttcg acgtcgggca
     1021 tatcgatgcc ttcgctggac atatatttgc gtagccgctc ggttaaatcg atcggcatct
     1081 gcttctcgta gatcaccctg acatccgacg gatcgacttt agcactatta tgcgcggtaa
     1141 atctgatctt gagctcgaac ggcgaatgga cggaggtcgt cgggctcggc aatatctgat
     1201 cgacggatgg tccgcgcgtg ataccgcgca catttatgcg gacatcgctg ctaggtggtt
     1261 ggaccgcctc actcgcggtg atgagaactt cagcgcgcgt atgggcgcca agcaacagca
     1321 acgcgctgca ggtcgtcaca atgatgcgca tcccgatctc catgacctgc actgctgatg
     1381 ctccaccttc tattctcctt cagaggaatt ggccaagtcc actccagatc gacaccagcg
     1441 cggagacacc tgcaatagaa gctgtaatga tcttttccgc caagccaacc cgcttgagat
     1501 atttgtccat ccgtgggtcg ttggggtctt gagggcttac gccgctcact ttcgctgtcg
     1561 catatttggc ggcaaagtaa acgaacctgc tcgtctttgc ggtcggcgtg ctcatagcaa
     1621 tggcggtctg ccggcacacc gcaccggcga aaaatgctag cgcgatgctg gcgccatatt
     1681 cgaggtactc gagccacccc tgccgatcca tcggcagcac cggaaccctg tcgacctctg
     1741 caactaccgc cgacatcgca aatatcgctg ccgccgcgag caaaattcca gcggcgaggt
     1801 cggacgacaa atactcggtt gtattctgcc gataaataaa cccgaatacg aacggcaaaa
     1861 ctaacgaaac aatgtgcaac caaaacagag agagatcata atggatcacg atgatgaaat
     1921 gcgcgagcaa caaagcgccg agtgccagca gcacggttgc cagcggtgaa agaagcggaa
     1981 tatggcgttc aatggcgccg gcgataacag cgactcgctc ttgatgagca ccttggggga
     2041 taggtgcgtg tgtaatcgac tcctcgcgag tcgccgccgg gtcgctgata acgcttcgaa
     2101 attgtttggt cagatcttcg atgcgctcca tcagtgcgcg gacaacactg aggtcatgcc
     2161 ggcaatgttt gcagacgatc gcttcgtctt tgacctcttc agcgcaaaac gggcatttca
     2221 tgaaaaccgt ctttcgccaa tgcattagcg gatgtcagcc accatcgcca atcaacgaat
     2281 aaggagccca gaacagagga tgcgcgtagc tgaaaatgct gtgacccgaa cgatcgaccg
     2341 tgcctggtcc gtcgagcagc gccatcatcg cttggcgcag ggcttcactg cgagagatcg
     2401 acgaatccgc gctctgccgc cggaatacat cggagatcaa ttcgcgcgcc gaaaccgaat
     2461 gcacagacca attggtgacc agtaatgcgc gcgttccagc gtagaagaag gcgctgccaa
     2521 ggcctgacgc cgcttcggcg ccggctcccg ctcccgctcc cgcagcggta ttacaggccg
     2581 acagcacgac ccaatcagcg tcaagtttta aggcgaggat tttctccatt gtcaggaggc
     2641 cgtcaccttt gactcccgca acttcgggtg cggtcagcgc caatgccggt tgggtgagcc
     2701 cgtcgacatc gccaggaacg agaccatgtg ttgcgaatgc gattatctgg tagtgcgaca
     2761 aattgatggt ctcgacgttc tgctcgttgg catccctgcc gagataaagc gacttggctg
     2821 gatcgacatc gagcgcctta gccactgcat gcaattccag gcgcgtgtcc ggcagcctcg
     2881 gtagcaacgc aagctctgcc gtatcgacgt cctcgatatg gggcgccgcg cgtaatttga
     2941 gcggaatgcc ccgcgtcgtt accgcatcgt catcagacgt ctccgcggaa gcgacctgca
     3001 tcggaacctg ctgctccgcg ttcgcatctg ccgcttcctg tgcgttgaaa tagggatcgc
     3061 cgaagccgat caatttgtcg cggctggaag tgcccggcgg gagatgccgc agcgtgatca
     3121 gtgctgaagc cgacgggacc accgtgactg catgggttcg cgcgagccac ggcacattgc
     3181 gatagccagc aaacagcggt ttcgcggcca gatcgacttg cgctggcgcg gtgggcaata
     3241 atcctagcgg caatgcgcca agcgcgccat tcgtcacgac gattaggctc tttgaacccc
     3301 gccaaccggt ttcaaccggc ttgagcagcg tcgcgtaaag ctggtaggcc agcgcgacat
     3361 cgaatgccgg gatttccgat accatcgtaa cctgcggttc gagcgcctcg cgtagtttgc
     3421 ggacattggc ctcaagctgg accgccgtcg ccggcaccgc ggcgaatgcg actgcgccct
     3481 ccttaggcac tgcccagaca aagctcttgt cctggccaaa ataaaatgac agcaacgcct
     3541 cgcccgaccg cagcgccgcc ttgatttcgt caaccgtcgg cggtttcggg tcgatgagag
     3601 tggcataggc cgggaagcgc tgcttgatct gctgctgggc cgttttgcgc tcggtgcgca
     3661 gttgttcaat ttcggttctg atcgcgtgca cagtctggct gtcgcgctgg tccgacggca
     3721 gcgcgaggag attattgagc gtacccagtt cggcactgat ttgcttggcg cggtcctgtt
     3781 cggtgcgcac tagttcggcg agcgccgcgt ccttcgccac catgcgtgcg ctcgaatccg
     3841 ctagcgcctg ctgcaccgcc tgaccgcgga ccgcgtcggc aagcccgaaa gtctcaaccg
     3901 cgacatcatt tgacgcgttc tgactgcgcg ctagaacagc gatgtaagcc tcgacaattc
     3961 tttgcagacg ggcgttgcgc gccgccacca gggtcggatc attattgtcg gtttctcgtg
     4021 ccgccgacat cagcatcgga accgccacct tgaattcggc gatcgcgtca gcgtcgcgcc
     4081 ctgcccgggc atagccttca gcaagcgtgc cgtgcgccga tgccgcatcg aagctgttcg
     4141 cgccggtgcg ggcgatttgg cgtttgacca gcgcttcggc cccggcaatt ccggcttcga
     4201 actggcccga cgcatagagt gcggcaatgc gcgagccgtt tagctcgaac gcttcgcgct
     4261 gttgtggccc ccattgctcc atggctttgt cgagttgcgc atagacgatg gcggcctctt
     4321 tcgctttacg ctcagcgacg aggatgttcc caagtcccga gagcatcgcc acgctttggg
     4381 gcgcatcgtc gcgaatgccg agcgtgcgct gaatgtcgag ggccgagcga gcgagcttct
     4441 cagcttcctg gtaacggccc tgttcgacca ggatggcggc gagaccaacg atgaattgtg
     4501 gggtcgtcgg cgtgtactta ccttgggcct tgaggacgcc aagcagtgcg cggcgggcgt
     4561 ctgcttcagc ttcgctaagc ttcccctctc tagactcatt gcgggcggcc gcaagaaggt
     4621 gagaatctgc ggccagcatc atctgttctg caggcggcgg ataatcgtac cgaggcagat
     4681 ccttgatccc ggcgcggcgg aacgcctctg cacgccggta cgccgcctcg gcctcctgat
     4741 attggccgcg cgcctcgaac accagtccgc gcgctctgtc ggcgtcggct tcccaggaat
     4801 gaccgtagac gctataggac tgtcgccagc tcggattggg actgccgcgc gcttcctgga
     4861 caagtgcctc gacccggccg gcgtaggtgc cggcctggct gatgtcgccc atcataatcc
     4921 atgtctgcgc tatatttgct aaggcgttga ccatactgcc ccggtgacct ggttggtcac
     4981 cctctcgcgc catcgaatcg aatatcgcca tcgcttgctt gggatccccg atagtccggt
     5041 attgcaaagc gacgaattg
//
LOCUS       DATLJT010000450         1853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2553831,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000450 DATLJT010000000
VERSION     DATLJT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553831"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VM910_10370"
     CDS             complement(<1..213)
                     /locus_tag="VM910_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62972.1"
                     /translation="MSALVILQREVRGSARLWLLVAIGFFVGFQLLQLALLIGRFQVF
                     PNYLIVHDWFANIARIVRMTPSVGDMI"
     gene            complement(173..652)
                     /locus_tag="VM910_10375"
     CDS             complement(173..652)
                     /locus_tag="VM910_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62973.1"
                     /translation="MTIRSKALSLVNVHARIAPEPTAKMAVVDDSADVAVTAPAATAE
                     IERSRSSQPDAREEPSERMAGITLSAARWAVRLSALLAGLVCWHYASSKGLTLYVRFE
                     QIPGPLKVGEALINQLQHVAFYAATTALVHLITRPALELGRGRPDERPRHPAARGSR"
     gene            complement(649..1194)
                     /locus_tag="VM910_10380"
     CDS             complement(649..1194)
                     /locus_tag="VM910_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62974.1"
                     /translation="MSRKRVLYLYFSKGGHLTQDPTLKKQWVDTLKVDHQVLVNDNLA
                     PALDFDAWIKEDYLKRAYAEMGVDYEAEKAKVVDTLVTNAGRPSEIWHARDGILSYPT
                     LKEFLKAVAEFRATGAKLNATYVYDQATGLKLFGKTAFFVHKPDGDFATFLRKGEAEE
                     FAAKSGGKVVSFEEAVASSAS"
     gene            complement(1280..1768)
                     /locus_tag="VM910_10385"
                     /pseudo
     CDS             complement(1280..1768)
                     /locus_tag="VM910_10385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
BASE COUNT          349 a          552 c          564 g          388 t
ORIGIN      
        1 aatcatgtcg cccacggatg gcgtcatgcg gacgatcctg gcgatgttgg cgaaccagtc
       61 gtgtacgatc agataattag gaaacacctg gaagcgccca atcaagagcg cgagttgcag
      121 aagctggaag ccgacgaaga agccgatagc cacaagaagc cacagccggg cgctaccgcg
      181 aacctcgcgc tgcaggatga cgagggcgct catctggcct tcctctgcca agctctagcg
      241 ccggacgtgt gatcaggtgc accagcgccg tggtggcggc gtagaacgca acgtgctgca
      301 gttggttgat cagcgcttcg ccgaccttga gcggaccggg gatttgctca aaccgaacgt
      361 ataaagtgag tcccttggaa ctcgcgtagt gccagcaaac gagtccggcc aacagcgccg
      421 acagccgcac cgcccagcgg gcagccgaca gcgtaatgcc agccatccgt tctgatggct
      481 cctcgcgcgc atcgggctgc gacgaacgtg acctttcaat ctcggcggtc gccgccggcg
      541 cggtcaccgc gacgtcagcg ctgtcatcga cgaccgccat tttcgctgtc ggttccggag
      601 caatgcgtgc gtgcacgttc acgagtgaga gcgccttgct tcggattgtc atgacgccga
      661 gcttgcgacg gcctcctcga acgagacgac ctttccgccg ctttttgcgg cgaattcctc
      721 ggcctcaccc ttgcgcaaaa aggtcgcgaa gtcgccgtcg ggcttgtgga cgaagaacgc
      781 ggtcttgccg aacagcttga gccccgttgc ctgatcgtag acgtaggtcg cgttgagctt
      841 ggcgcccgtc gcccggaatt ctgcaaccgc cttgaggaac tcttttaatg tcggataaga
      901 gaggatgccg tcgcgcgcgt gccagatttc gcttggtcgg cccgcgttcg tcaccagcgt
      961 atcgacgacc ttcgcctttt cggcttcgta gtctactccc atctcggcgt aggcacgctt
     1021 gagatagtcc tccttgatcc aggcgtcgaa gtccaaggcg ggagcaaggt tgtcgtttac
     1081 aaggacctga tgatcgacct tgagcgtatc gacccactgc ttcttgagcg tcggatcctg
     1141 cgtgagatgg ccccccttgg agaaatagag gtagagcacc ctctttctcg acaccggtcc
     1201 acttttccat cagatcggtc gcccgcatgg gatcctcatc gatccatttg ccggcgtcgt
     1261 agaccgcctt gacgaaggcg acgaccacct ccggatattt ctcggcgaag tcctgtcgca
     1321 cgacgacgcc gtgcagatag ggaattgcga gctcgctgcc gtcatagatc tttcggccgg
     1381 tgccgcggaa ttccatgatc tccgaccatg ggcagaagtc cgagtgggcg tcgaccttgt
     1441 tggtcgcgat attgctagcg cctacgggag gcgtctgatt tttgagatcg atgcttgcga
     1501 gcggcatctt tttgacctgc aaagccttga ccagcatgcc ccaggaggcg ctgccgaccg
     1561 gcgtcgagat cgatttcccc gtcaggtcct cgagcttgta gtaattggaa gcgaccggaa
     1621 cgacgattgc gttgccggct cctttgagat tgtagccggt gcccgtgaac aggaccgtgc
     1681 gcaggctctt ggtctcctga aacttcgcgc cattcacgat cagcggataa tcgcccatgg
     1741 tgccgaaatg gagtttgccg gcgagcatct gattggtgat cgggccgccc gacggatagt
     1801 cggcccagga gatgttgtag gtagcgtcgg cgtacttgcc ggtgtgcggc agg
//
LOCUS       DATLJT010000451         1686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2094018,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000451 DATLJT010000000
VERSION     DATLJT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094018"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VM910_10390"
     CDS             complement(<1..348)
                     /locus_tag="VM910_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62975.1"
                     /translation="MHSVALGVVAAAAMSATTAIAQTRLVTEEMMVKSPDPGIEIYVR
                     NKRPAQMTTFRPERTVLYVHGATYPASTAFDLQLGGMSWMDYIAARGYDVYLLDLRGY
                     GKSTRPKEMSEDPK"
     gene            482..>1686
                     /locus_tag="VM910_10395"
     CDS             482..>1686
                     /locus_tag="VM910_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI62976.1"
                     /translation="MIKLTRRRLLRLTAGPAALPIVSRVAMAQDNPSPPRERPLAERL
                     AAYAHSLRHDDLDAVTIERLKVHVIDTIGCGIGAFEEKAVRICREIALAVPGTATVIG
                     TSRRTTPDLASFANGAAFRYLDFNDTYVGRFAVHPSDTIAACLAVAEAERANAQDLMT
                     AIALAYEVNSRLVDSLDIAARGWDPPVFSLPAVALAAGKLMKLDPERLTHAVGLAIND
                     HIPMAQTRIQTLSDWKGLAAAEAARNAVFAALLARGGVTGPTPIFEGRSGFFKQVSGP
                     GNVDVETFGRRGVPFRIHQCSLKAYPAVIYTQTAIVAGIEVGKEAGSLDQIASIEIAT
                     TGRGFERTGSEPEKWSPQTRETADHSLPYITARAMVDGDITNESFAPQMYRDPRILAF
                     MQKIKVSEDS"
BASE COUNT          324 a          524 c          523 g          315 t
ORIGIN      
        1 cttgggatct tccgacattt ccttcgggcg tgtggatttg ccgtagccgc gcagatcgag
       61 aaggtagacg tcgtaaccgc gagcggcgat gtagtccatc catgacattc caccgagctg
      121 caggtcgaag gccgtgctcg caggataggt tgcgccgtgc acgtagagta cggtgcgctc
      181 gggacggaac gtggtcatct gcgccgggcg cttgttgcgt acgtagatct cgatccccgg
      241 atcgggagat ttcaccatca tctcttcggt aacgaggcgc gtctgcgcga ttgcagtggt
      301 cgcggacatg gcggcagcag cgacaacacc gagagcaaca gagtgcattt tcacgatatc
      361 ctccccgtcg attgcgccgg cgcttacccc aaggccaaga tcacggagtg actatagcag
      421 gtctggagcg gctcgggcgt gcatctggcg caccgattgg cgagatttct aggaggaaat
      481 cgtgattaaa cttacccgca ggcgacttct gcgcttgacc gccggtccag cggcgctgcc
      541 catcgtttcg cgagttgcca tggcgcaaga caatccatca ccaccacgtg agcgaccgct
      601 tgcggaaagg ctggccgcct atgcccattc gctacgccac gacgacctcg atgccgtgac
      661 catcgagcgg ctcaaggtgc acgtcatcga tacgatcggc tgcggtatcg gagcgttcga
      721 ggaaaaagcc gtccgtatct gccgcgagat tgcgcttgcc gtgcccggta ccgccacggt
      781 catcggaacg agccggcgca cgacgcccga tcttgcgtcc ttcgccaatg gcgccgcgtt
      841 ccgctacctc gatttcaacg acacgtatgt cgggcgattt gccgttcatc cgagcgacac
      901 catcgcggcc tgccttgcag tcgcggaggc cgaacgggcc aacgcccaag atttgatgac
      961 cgcgatcgcg cttgcctatg aagtgaactc ccgcttggtg gattccttgg acatcgcggc
     1021 gcgcggttgg gatccgccgg tgttcagcct gccagccgtg gcgctcgcgg caggcaagct
     1081 gatgaagctc gaccccgagc ggctaaccca cgcggtcggg cttgccatca acgaccatat
     1141 cccgatggcc cagacccgga tacagactct gtccgactgg aagggactcg cggccgccga
     1201 agcggcgcgc aatgccgttt tcgccgcgct gctggcacgc ggcggcgtga ctggcccgac
     1261 gcccatcttt gaaggccgct ccggcttctt caagcaggtg tctggaccgg gcaatgtcga
     1321 cgtcgagacc ttcggtcggc gtggcgttcc gtttcgcatc caccaatgca gtctgaaggc
     1381 ttatccggcg gtcatttaca cgcagacggc gatcgtggcc ggcatcgaag ttggcaaaga
     1441 agcgggctcg ctcgatcaga ttgcctcaat cgaaatcgcg acgacgggcc gtggctttga
     1501 gcggaccggg agcgaacccg agaaatggtc gccgcaaacc agggagaccg cggaccacag
     1561 cctgccgtat ataacggcgc gtgcgatggt tgacggcgac atcacgaatg aaagcttcgc
     1621 gccgcaaatg taccgcgatc cgcgcatcct cgccttcatg caaaagatca aagtgagcga
     1681 ggactc
//
LOCUS       DATLJT010000452         1876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2323780,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000452 DATLJT010000000
VERSION     DATLJT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323780"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VM910_10400"
     CDS             complement(<1..1025)
                     /locus_tag="VM910_10400"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVI62977.1"
                     /translation="MTTEAVIVSTARTGVGKAYRGALNNTDGPTMAGHVMAEAVKRAR
                     IEPGEVEDVVMGCAMQQGTMVMNVARKGAIRAGLPVTVAGTTIDRQCASGLQAIAVAA
                     RSVMLDGVEIAIGGGIESISLVQNEHMNTFHAVDEELVALKPEMYMSMLETAEVVAER
                     YKIGRDKQDEYSLECQRRVGAALQGGRFNDEIVPITTKMAVVNKDTKEVSYQPVTLTK
                     DEGPRPDTTAEGLAKIKPVFEGKTISAGNASQLSDGASACVIMSDKIAAKKSLKPLGI
                     FRGFVAAGVEPDEMGVGPVAAIPRLLKRHNLKIDDIDLWELNEAYAVQVIYCRDKLGI
                     DPEKLNV"
     gene            complement(1040..>1876)
                     /locus_tag="VM910_10405"
     CDS             complement(1040..>1876)
                     /locus_tag="VM910_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVI62978.1"
                     /translation="EQTGSEKQKRAFLPDIIAGKKIWALAWTEKASRFDLAKVTTTAH
                     REGKDYVLTGEKSAVIAAPWADYLIVSARASGQRHDRGGVSLFVVDRRAANLHLQSFK
                     TIDGRRAAEISLGGVRGQLLGKEGEGVAALEACRDHAIGALCAEAVGAMGEMNSVTLE
                     YSKTRKQFGTTIGSFQVLQHRMVDMFIAHQEALSLMQHLNLSLSADDAGLSRLASGAK
                     SKIGYAGRFIADQAVQLHGGMGMTDELNVGHFFKRISSINIQFGDPAYHVLRYAQLDA
                     AA"
BASE COUNT          300 a          603 c          582 g          391 t
ORIGIN      
        1 ttgacgttca gcttctccgg atcgatgccg agcttgtcgc ggcaatagat cacctgcacc
       61 gcataggcct cattgagctc ccagaggtcg atgtcatcga tcttcaggtt atgccgcttc
      121 aagagccgcg ggatcgcagc caccggaccg acgcccattt cgtccggctc gacgccggcg
      181 gcgacgaagc cgcggaagat gccgagcggt tttaggctct ttttcgctgc gatcttgtcg
      241 ctcatgatca cgcaggccga ggcgccgtcc gaaagctggc tggcattgcc ggcgctgatg
      301 gtcttgcctt cgaacaccgg cttgatcttt gccaaccctt cggccgtggt atcgggccgc
      361 gggccttcgt ccttggtcag cgtcaccggt tggtagctga cctccttggt gtctttgttg
      421 accacggcca ttttggtcgt gatcggcacg atctcgtcat tgaagcggcc gccctgcagc
      481 gcggctccga cgcgacgctg gcactcgagc gagtattcgt cctgcttgtc ccgaccgatc
      541 ttgtagcgct cagcgaccac ctcggcggtc tcgagcatgg acatgtacat ttccggtttc
      601 agcgccacca gctcttcgtc gacggcgtgg aacgtgttca tgtgctcgtt ctgcaccagg
      661 ctgatcgact cgatgccgcc gccgatcgcg atctcgacgc cgtcgagcat caccgagcgg
      721 gcggcgaccg cgatcgcctg caggccagag gcacattgcc ggtcgatggt ggtgccggcg
      781 accgtcactg gaaggccggc acggatcgcg cccttgcgcg cgacgttcat caccatggta
      841 ccctgctgca tggcgcagcc catcaccacg tcctcaacct cgccgggctc gattcgtgcg
      901 cgcttcaccg cttcggccat cacgtgaccg gccatggtcg ggccgtcggt gttgttgagc
      961 gcgccgcgat aggccttgcc gacgccagtg cgcgcggtgg aaacgattac tgcttcagtc
     1021 gtcatgtttg cctctttgtt taagccgccg cgtcgagctg cgcgtaacgc agcacgtgat
     1081 aggccggatc gccgaactgg atgttgatgg aggaaattcg cttgaagaaa tgccctacgt
     1141 tcaactcgtc ggtcatgccc atgccgccgt ggagttggac cgcctggtcg gcgatgaacc
     1201 tgccggcgta gccgatcttc gacttggcac cggaggcaag tcgcgagagc ccggcgtcat
     1261 cggcgctgag gctaagattg agatgctgca tcagggagag tgcctcctga tgcgcgatga
     1321 acatatcgac catccgatgc tgcaggacct ggaaggagcc gatcgttgtc ccgaactgct
     1381 ttcgtgtctt ggaatattcc agcgtcacag agttcatttc acccattgca ccgaccgcct
     1441 ctgcgcagag cgcgccgatg gcgtggtcgc ggcaggcttc cagcgcggca acgccctcgc
     1501 cttccttacc caacaactgg cccctgacgc cgcccaggct gatctcggcg gcgcggcggc
     1561 cgtcgatggt cttgaaactc tgcagatgca gattggcagc acggcgatcg accacgaaca
     1621 ggctgacgcc gccgcgatcg tggcgctgcc cggaggcgcg ggcggagacg atcagatagt
     1681 cagcccaggg cgcggcgatg acggcgctct tctcgccggt caggacgtag tccttgccct
     1741 cacggtgcgc cgtggtcgtg accttggcga gatcgaagcg cgaggccttc tccgtccagg
     1801 cgagcgccca gatctttttg cccgcgatga tgtcggggag gaacgcacgc ttttgcttct
     1861 ctgatcccgt ctgttc
//
LOCUS       DATLJT010000453         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4391448,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000453 DATLJT010000000
VERSION     DATLJT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4391448"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VM910_10410"
     CDS             <1..443
                     /locus_tag="VM910_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVI62979.1"
                     /translation="YYAEKQLVKALQEMAGKATDKQLKDGFLAHLDETRNHVQRLEQV
                     FKMHGAAAKAIDCPAIDGIIEEANEVAGDVADKAVLDAALINAGQAAEHYEIARYGSL
                     IAWAKQLGRNDCASILQRTLEEEKAADKKLTQIAESNVNIRAAS"
     gene            complement(523..867)
                     /locus_tag="VM910_10415"
     CDS             complement(523..867)
                     /locus_tag="VM910_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62980.1"
                     /translation="MTTSDCARSTRSTALKESGSTATIGAVACMIGALFAGSTVVTPL
                     YVMYKQQFGFSQISLTLIYAVYVAGNALPVIGIGVISTLGGPNVASLAFAATIAAFAL
                     VALFFGARYLPR"
     gene            1100..2506
                     /locus_tag="VM910_10420"
     CDS             1100..2506
                     /locus_tag="VM910_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI62981.1"
                     /translation="MMVPDRRADRSLLATSLTLAAMSLGYGVVQLDVTIVNTALSSIG
                     ASLGGGVSELQWVVSAYTIAFAAFILTAGALGDRLGAKRVFMTGFALFTAASLACALS
                     PNAVSLIAARCVQGLAAAILVPNSLALLSHAYDEDKARGRAVAVWAAGASLALTAGPF
                     VGGALITLVGWRSIFLVNLPIGLAGLWLTRRFAEETARRPQREIDLPGQAAAITALGA
                     LAAALIEGGARGWDSPLVIALFVVAALVGALFVWREARASQPMLPLSLFAHRMFALTA
                     LVGLLVNIAYYGLIFVLSLYFQEVNGLSPFATGLAFVPMLGTVLPVNLVAPRLAERIG
                     GPATIGIGAALSSLGCLAMLNIAPGTSYWQLCIQMIVLSAGLGLLVPPLTSALLGSVE
                     KSRSGIAAGVLNATRQTGSVLGVALFGSLIGNASVFMAGTHASLVISAVVLAVAAGAI
                     GWGGAAKERTRAERAQPT"
BASE COUNT          457 a          830 c          882 g          478 t
ORIGIN      
        1 tctattatgc cgagaagcaa ctcgtgaagg cgcttcagga gatggccggc aaggccaccg
       61 acaagcagct caaggacggc ttcctggccc atctcgatga aaccaggaat catgtgcaac
      121 ggctcgaaca ggtgttcaag atgcatggcg cggcggcgaa ggcgatcgat tgcccggcca
      181 tcgacggcat catcgaggag gcgaatgagg tcgcgggcga tgtcgccgac aaggcggtgc
      241 tggacgccgc gttgatcaac gcaggccagg ccgccgagca ttacgagatt gcgcgctatg
      301 gcagcctgat tgcctgggcg aaacagcttg ggcgcaacga ctgcgccagc attctgcagc
      361 ggacactgga ggaggaaaaa gcggcggaca agaaactgac gcagattgcg gaaagcaacg
      421 tcaacatccg cgccgcgagc tgattgcagt cagacaacca gcttgagaaa gacgtcgcgc
      481 aaggagcctt gcgcgacgtt ttgccttgcc gaccggaccc gtttacctcg gcagatatcg
      541 cgcaccgaag aacaaggcca cgagcgcaaa agcggcaatg gtcgcggcaa acgcgaggct
      601 cgcgacattt ggaccgccga gcgtcgagat gacgccgatc ccgatcactg gcagagcgtt
      661 tccggcgaca tagaccgcgt agatcagggt gagcgagatc tgcgagaagc cgaactgctg
      721 cttgtacatg acataaagcg gcgtgacgac cgtgctgccg gcgaacagtg cgccaatcat
      781 gcaagcgacc gcgccgatcg tcgcggtgct gccggactcc ttcagtgccg tcgagcgggt
      841 ggatcgggcg cagtcgcttg tcgtcatcgg atttctccgc cgctgaacga tgaccagaag
      901 agagaacaac ttgtgctctg cattctggcc gatcgtcacg gcccgaatag ctgaagctcc
      961 accgagtgtc atgcctttgc agcaatagtt cgatcgagac cgaagcgttg tgggaaacgg
     1021 aaacctatat gcagcgttgc ccctcgacat gaggtgaacc atgcgaactg cgtccatgaa
     1081 aatcggccct gtttcgacga tgatggttcc ggacaggcgc gccgatcgtt ccctgcttgc
     1141 gacctcgctg acacttgcgg caatgagcct tggttacggc gtggtccagc tcgacgtcac
     1201 gatcgtcaac actgcgctct cgagcattgg cgcttcgctc ggcggcggcg tgtcggagct
     1261 gcaatgggtc gtcagcgcct acaccatcgc ctttgccgcc ttcattttga ccgccggtgc
     1321 gctcggcgat cgcctcggcg cgaaacgagt gttcatgaca ggctttgcgc tgttcactgc
     1381 ggcctcgctc gcctgcgcgc tttcgcccaa tgccgtgagc ctgattgcgg cgcgctgcgt
     1441 gcaggggctt gcggcagcga tattggtgcc gaactcgctg gcgctactca gccatgccta
     1501 tgacgaagac aaggcgcgcg gccgcgcggt cgcggtgtgg gccgccggcg caagccttgc
     1561 actgacggcg ggcccctttg tcggcggcgc gctgatcacg ctggtcggct ggcgctcgat
     1621 ctttctggtc aaccttccga ttgggctggc cgggctgtgg ctgacccgac gctttgccga
     1681 ggagaccgcg cgccgcccgc agcgggaaat cgacttgccg gggcaggctg cggccattac
     1741 cgcgctcggc gctttggcgg ccgcgctgat cgaaggcggg gcgcgcggtt gggacagtcc
     1801 gctcgtgatc gcactcttcg tcgttgcggc gctcgtcggc gcgctgttcg tctggcgcga
     1861 ggcgcgcgcg tcgcagccga tgctgccgct gtcgctcttc gcacatcgga tgttcgccct
     1921 gaccgcgctg gtcgggctgt tggtcaatat cgcctattac ggactgatct tcgtgctcag
     1981 cctgtacttc caggaggtca acgggctatc cccattcgca accggcctgg ccttcgtgcc
     2041 gatgctgggc acggtcttgc cggtcaacct ggttgcgccg cgactggcgg agcggatcgg
     2101 tggacccgcc acgatcggca tcggcgccgc cctttcgtcg ctcggctgtc tggcgatgct
     2161 caacatcgcg ccgggaacga gctattggca actttgcatc cagatgatcg tcctcagcgc
     2221 cgggctgggg ctgctggtcc cgccgctgac gtcggcgctg cttggcagcg tggagaaatc
     2281 gcgttccggg attgccgccg gcgttctcaa cgcgacgcgg caaaccggca gcgtgctcgg
     2341 cgtggccctg ttcgggtcgc tgatcggcaa cgcgtctgtc ttcatggcgg gcacgcacgc
     2401 ctcgctcgtg atctccgctg ttgtccttgc cgtcgcggcc ggcgcgatcg ggtggggcgg
     2461 cgcggcaaaa gagcggacgc gcgccgagcg cgcgcagccg acgtgaccgg tcgagtactc
     2521 aggcctcgcg cagcgcaaaa ttcgcgccgc agaacgccat gacggcaccg aggcaaaggg
     2581 cggcgacgcc ggccagcacg ctggaaaagg ttggacccgc gctcggcgcc gatgcgacct
     2641 gcccggc
//
LOCUS       DATLJT010000454         8743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3243482,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000454 DATLJT010000000
VERSION     DATLJT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243482"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM910_10425"
     CDS             <1..274
                     /locus_tag="VM910_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62982.1"
                     /translation="RTRIRGKATIHDGAEMLARHHGAKVVVRVKCEIFPNCPRYIPDL
                     MGASAAVPANPFVPRPGYEPPPPEWKSRDYLKDELSKYDPHRKRET"
     gene            complement(225..1229)
                     /locus_tag="VM910_10430"
     CDS             complement(225..1229)
                     /locus_tag="VM910_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI62983.1"
                     /translation="MISIFVVATLGLLLLATQIGVVMIERAFPIRGRAVEVTGAVLNV
                     VELGPREAAGPPILLLHGASSNLEAMRQPLGDMLARNRRVILIDRPGHGFSTREREID
                     STPAIQARMIGEALEELGVGPVIVVAHSWAGALAARMALDEPRHVAGLVMLAPVTHPW
                     KGGVGWYNKAVTTPVIGPLLAHTVTLPLGLLLTKPGARGVFLPQPMPDGFVNDTATWL
                     LLRPREFLANARDLVALKQTVIEQAPRYGEIKVPVAIISGDIDKTVSTNIHSRPFAAA
                     VPGAKLLVLPGIGHMIQNAAPDLVVSEVEAMMGLAVVTWAPTAMSRVSDADRTSRARL
                     "
     gene            1498..1680
                     /locus_tag="VM910_10435"
     CDS             1498..1680
                     /locus_tag="VM910_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62984.1"
                     /translation="MSFRSSEAAIDEIVASCDGDLRGALRALLLVNEHLEAEIAQLYA
                     AAAHYGHSERGGNALH"
     gene            complement(1705..1950)
                     /locus_tag="VM910_10440"
     CDS             complement(1705..1950)
                     /locus_tag="VM910_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62985.1"
                     /translation="MKALMLTGAIVLAAGPADTGDKLYWVVGKREAGKCEIVTSNPVV
                     IGDTWFADGPYKSSDDAKLARSTIPECPPVKDEPAGK"
     gene            complement(2096..2740)
                     /locus_tag="VM910_10445"
     CDS             complement(2096..2740)
                     /locus_tag="VM910_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI62986.1"
                     /translation="MALRFRKAIFAAAGLAVILAVVPSLALAQDDPRALSRKKLPEAP
                     SKLPKVGADKTRGGLDFLFGALKAAPDDASARHVEARIWALWLQTPSDTAALLMMRAK
                     AAMDAQQIDVALKLLDAIIKLRPDWTEAWNRRATLYYLQNDYARSLADIQQVLAREPR
                     HFGALAGLGMIMEDLGDDKRALEAFRKALAVNPHLEKVPELVRQLSEKVEGRDI"
     gene            complement(2832..2957)
                     /gene="ykgO"
                     /locus_tag="VM910_10450"
     CDS             complement(2832..2957)
                     /gene="ykgO"
                     /locus_tag="VM910_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HVI62987.1"
                     /translation="MKVRNSLKSLRGRHRANRLVRRKGRVYVINKVQRRFKARQG"
     gene            3226..4110
                     /locus_tag="VM910_10455"
     CDS             3226..4110
                     /locus_tag="VM910_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI62988.1"
                     /translation="MPKLKLPDIENVIAIDIHTHAEEPCGLHADDGYDDFQAKMAEYF
                     KSPNKHPPTVPETAAYYRAKKIAAVIFPVDAERETGFRRYSNYEMLEVASENLDVLIP
                     FVSIDPHKGKLGVREAKKLIEEYGVRGFKFHPTMQGFYPNDRMAYPLYEAINDAGAIA
                     LFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVAADFPDLKIILAHPSFPWQEEALSVA
                     THKPNVYIDLSGWSPKYFPPILVRYINSILQDKMLFGSDWPVITPDRWLADFAKLEIR
                     EEIRPKVLKANARRILGI"
     gene            4150..5007
                     /locus_tag="VM910_10460"
     CDS             4150..5007
                     /locus_tag="VM910_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI62989.1"
                     /translation="MRNPFGAIVFVTALLVGSATVCHAAGPESAGTVVELETPLASPH
                     PLQGYLRKPGGSGALPAVVLLGSCVGNWRRLDERWGKLIASWGYVTLTVDSLGPRGLQ
                     STCTGAPSDDFAFDAYRALKFLAQRSFVDPARVAALGFSSLGGWIVLSSVERGTIERN
                     SQDKFRAAVAFYPPCLGFKGDMTVPTLIMIGELDDWTTATACRNMVDGKDDWGISRQK
                     GNGVPVRLIVYPGAYHGFDAPNLKTPSDMFGHHLEFNQAAADRSIAALREFLDWTIGA
                     RSQGQALAK"
     gene            5048..5794
                     /locus_tag="VM910_10465"
     CDS             5048..5794
                     /locus_tag="VM910_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVI62990.1"
                     /translation="MTIKAVVFDAYGTLYDIQSVAEITEDAFPGYGEIITQIWRIKQL
                     EYTWLRSLMRRYQDFSHITQDSLAYTLRCLGLQHDRDTFERIMEKYLHLDLYPDALAA
                     LAALKGNKLAILSNGSPEMLNALVKNTGLDRVLDATLSVDPKKVFKPAPDAYTLIEEE
                     LGVAPREVLFVSSNPWDACGAESFGLNVAWIERVTPGSMALLCVENETVAPLTMFKAL
                     RTQMDELGVLPDHRIRSLSDLPKVVASYEP"
     gene            5784..6251
                     /locus_tag="VM910_10470"
     CDS             5784..6251
                     /locus_tag="VM910_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HVI62991.1"
                     /translation="MSLDSVRAFFAEKAPDITVIESQQSSATVVLAAEAYGVEPARIA
                     KTLSLRVGERVVLIVTSGTSRMDNRKVKALFGGKPKMLGLEEVADITGHEVGGVCPFG
                     LKAPLPIYCDVSLKAFDIVVPAAGSTHSAVRIAPARMAELTSAEWVDVCQNGD"
     gene            6363..7859
                     /locus_tag="VM910_10475"
     CDS             6363..7859
                     /locus_tag="VM910_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HVI62992.1"
                     /translation="MTITAQKILPRAHTGIPGLDDILEGGLARNRLHLLEGTPGTGKT
                     TIALQFLLAGASVGETGLYVSLAETEEELREGAASHGWRIDERVSICELVRPESVLDP
                     DQHQSLLYSSDLELGETVERMLEAIEKYKPQRIVIDSLSEIRLLAQSSLRYRRQILAL
                     KHYFARHKSTVITLDDLTTESTDGAVHSIAHSVIHLDQLAPVYGAERRRLRVTKCRGQ
                     SFRGGYHDFTIATRGVQVFPRLVAAEHRHAFAGTAIKSGIAELDSLLGGGLTAGSSTL
                     IIGPAGTGKSLLSLQFLASSVKRGERAGLFVFDEELGLLFSRAKSLGIDIEAMSKDGK
                     LFVQQLDAAELSPGEFTHRVRSCVDEQNIRTVAIDSLNGYQAAMPEEQFLILHLHELL
                     QYLNRRGAATLLTVAQHGMMGDMKQPIDVTYLADTVVMLRNFEAMGRVRRAISVIKKR
                     TGSHEDTIRELKINGNGINLGAPLVEFQGVLCGVPTYVGRRAPLMESS"
     gene            7856..>8743
                     /locus_tag="VM910_10480"
     CDS             7856..>8743
                     /locus_tag="VM910_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI62993.1"
                     /translation="MTKADHWEAVERTLILAPRGRDAALAESILREAHLRTEICKSFQ
                     ELFAGIKAGCELAIVTEEAFRSVDTRNLSEWVSRQPAWSDFPFIVLTEHGGGLERNPL
                     AAQLTEALGSVSFLERPFHPTTLISVVRTHLRGRRRQYECRKLNEELEVRVQERTHEL
                     AAANRQLLGQMEERERVESTLRQMQRLEAVGQLTSGVAHDFNNLLTVVLGNIAFMERD
                     FAARGIEGKLAQRLRFMRTAAERGAKLTDQLLSFSRRQRLEPKVLDLNHTIASMRDLL
                     QSTMGGIRIETSLADGIWHA"
BASE COUNT         1693 a         2756 c         2669 g         1625 t
ORIGIN      
        1 tcgcacgcgt atccgaggca aggcaacgat ccacgacggc gcggaaatgc ttgcccggca
       61 tcatggcgcg aaagtcgtcg tgcgcgtcaa atgcgagatc tttccaaact gcccgcgcta
      121 cattccggat ctgatgggcg catccgcggc cgtaccggcg aatccgtttg ttcctcgacc
      181 cggctacgag ccgccgccgc cggaatggaa aagccgggat tacctcaaag acgagctctc
      241 gaagtacgat ccgcatcgga aacgcgagac atagcggtcg gcgcccaggt gacgacagcg
      301 agccccatca tcgcctcgac ttccgacacc acaaggtccg gcgccgcgtt ctggatcata
      361 tggccgatgc cgggcaacac gagcagtttc gcgccgggca cggcagcggc gaagggacgc
      421 gaatggatgt tggtcgacac cgtcttgtcg atgtcgcccg agatgatcgc cacaggcacc
      481 ttgatctcgc catagcgcgg cgcctgctcg atgaccgtct gtttcagggc caccaggtcg
      541 cgcgcattgg ccagaaattc gcgcgggcgc aacaagagcc aagtcgctgt gtcgttcaca
      601 aagccgtccg gcatcggctg cggtagaaac acgccgcgcg cgccgggttt tgtcagcaac
      661 aggccgagcg gcagcgtgac agtgtgggcg agcagcggac cgatcacggg cgtagtgacg
      721 gccttgttgt accagccgac gccgcccttc cacggatgcg tcaccggcgc gagcatcaca
      781 aggcccgcga cgtggcgcgg ttcatccagc gccatccgcg cggcgagcgc gccggcccag
      841 gaatgcgcga cgacaatgac tggcccgacg ccgagttctt cgagcgcttc gccgatcatc
      901 cgcgcctgaa ttgcaggcgt cgaatctatc tcgcgctcgc gtgtgctgaa gccgtggccc
      961 ggccgatcga tcaggatgac gcggcgattg cgcgccagca tgtcgccgag cggctgccgc
     1021 attgcctcga ggttggaact cgccccgtgc agcagcagga tcggcggacc ggccgcctca
     1081 cgcggcccca gctcgaccac attcagcacg gcgccggtga cctcgaccgc ccttccccgg
     1141 attggaaaag cgcgttcgat catcacgacg ccgatttgcg tagccagcag cagtaaaccc
     1201 agggttgcca cgacgaagat tgaaatcatt gaacttttcc agacgaatgc cacacgcgga
     1261 agctacgcgg cggaacttga gacggtttcg cagggaataa agcgggcggt ttttccgaaa
     1321 aattacgcgg acctgtcata cggccgtcgt cggccatttt ataaccattg tcagcacttt
     1381 ttctgactgc cgggcgcgca attaccgaag actacccccc ggcctcgttc acatcctcac
     1441 acggaacacc ggatcggtcg cagagttatc ccgcagggac atttgaaggg gatttcgatg
     1501 agtttcagat cgagtgaagc cgcgatcgac gagatcgtgg caagttgcga cggcgacctg
     1561 cgcggcgcgc tcagggcgct gctgctcgtc aacgagcatc tggaggccga gatcgcgcag
     1621 ctttatgcgg cggccgcgca ttacggccat tccgaacgcg gcggcaacgc actgcattag
     1681 ctgcaggaga caagcaatcc gccatcactt gccggcgggt tcatctttca ccggcggaca
     1741 ttccggaatt gtcgagcggg cgagcttggc atcgtcgctg gacttgtagg gcccgtccgc
     1801 aaaccaggtg tcaccgatca cgaccggatt ggaggtcacg atctcgcact tgcccgcctc
     1861 gcgttttccg accacccaat agagcttgtc gccggtatcg gccgggcctg ccgccagaac
     1921 gatcgcaccc gtcagcatca aggctttcat gttgccgctc ctgtcgcatg acatgcggtg
     1981 aagcgcggct taacgaaaaa cgcgccgcct cacaatggat cgcctgagat tagggttacc
     2041 cgacgtctgc gtgtcgttcg caaatccgag cattgcgggc cgaagccaca gcgcttcaga
     2101 tgtcgcggcc ttcgactttt tcgctcaact gcctgaccag ttcgggcacc ttctccagat
     2161 gcgggttgac ggccagcgcc ttgcggaagg cttccagcgc gcgcttatcg tcgccgaggt
     2221 cctccatgat catgccgagg ccggcaagcg cgccgaaatg ccggggctcg cgggccagca
     2281 cctgctggat gtccgcgagc gagcgagcgt aatcgttttg caggtagtac agcgtcgcac
     2341 gccggttcca cgcttccgtc cagtcgggcc gcagcttgat gatggcgtcg agcagtttca
     2401 gcgcgacgtc gatttgctgc gcgtccatcg ccgccttggc gcgcatcatc agcagcgccg
     2461 ccgtatcgct cggcgtctgc aaccagagcg cccagatccg cgcctcgaca tgtcgcgcgc
     2521 tggcgtcgtc gggcgcggcc ttcagcgcgc cgaacaggaa atcaagcccg ccgcgcgtct
     2581 tgtcggcgcc gactttgggc aacttcgaag gcgcttcggg aagtttcttt ctggacagcg
     2641 cgcgcggatc gtcctgcgcc agcgcgagtg agggcacgac cgccaagatg accgccaagc
     2701 cagcggcagc gaagattgct ttccggaatc tcaatgccat ccgcgaagtc tagacgcgga
     2761 acgacgcgct tgcctagcag caaacgcgtc aaaggcgtgt gagaccgggg tttttccggg
     2821 ccgaaaaatg atcagccctg acgcgccttg aagcggcgct ggaccttgtt gatcacatag
     2881 acccggccct tgcggcggac caggcggttg gcgcggtgcc ggccgcgcag cgatttcagc
     2941 gagttacgga ccttcatgga cctgtcctga tgcgttgatg aggcccaagt tgaggccgga
     3001 tatcgcgaaa aattgggatt gtcccgtcca ccttcaggcc agtccgggac gccgcggttt
     3061 ctaagccatg gaacggccaa atgtcaatcc gaacagggcc ttttcggcga gttccctctg
     3121 cagggcgcgc cttttgggcc caaatccaga ggccaaattc ggccgttgcc aaatttgtta
     3181 tatgatataa tcaatttgcc aaatcgccgc catgggagaa cgcagatgcc caagctcaaa
     3241 ctccccgata tcgagaacgt catcgcgatc gacattcaca cccatgccga agagccatgc
     3301 gggctgcatg ccgatgacgg ctatgacgac ttccaggcga agatggcgga gtatttcaag
     3361 tcgccgaaca agcatccgcc gaccgtgccg gagactgcgg cctattaccg ggcgaagaag
     3421 atcgcggccg tgatcttccc cgtcgatgcc gagcgcgaga ccggtttccg ccgctacagc
     3481 aattacgaga tgctggaggt ggcttccgaa aatctcgacg tcctgatccc cttcgtctcg
     3541 atcgatccgc acaagggcaa gcttggcgtg cgcgaggcaa aaaagctgat cgaggagtat
     3601 ggcgtccgcg gcttcaagtt ccacccgacc atgcagggct tctatcccaa cgaccggatg
     3661 gcctatccgc tctatgaggc gatcaacgac gccggcgcga tcgcgctgtt ccacaccggc
     3721 caaaccggcg tcggctccgg catgcccggc ggcatgggca tgcgtctgaa atattccaac
     3781 ccgatgtata tggacgacgt cgcggcggat tttccggatt tgaagatcat cctcgcgcat
     3841 ccgtccttcc cgtggcagga ggaggcgctg tcggtcgcga cccacaagcc gaacgtctac
     3901 atcgacctgt cgggctggtc gccgaaatat ttcccgccga ttttggtgcg ctacatcaac
     3961 tcgatcctgc aggacaagat gctgttcggc tccgactggc ctgtcatcac gccggaccgg
     4021 tggctggcgg atttcgcaaa actcgagatc cgcgaggaga tccggccgaa ggtcctgaag
     4081 gccaacgcgc ggaggatttt gggtatatag tcaccttcgg ccaacggcag accttggcga
     4141 aggtgctttg tgagaaaccc tttcggggcc atcgtttttg tcaccgcgct cctggtcggc
     4201 agcgcgaccg tctgtcacgc ggccggtccg gaatcggcag gcaccgttgt cgagctggaa
     4261 acgccgcttg ccagcccgca tccgctgcaa ggttatctga gaaagcccgg cggcagtggt
     4321 gcattgcccg ctgtggtgct gctgggcagt tgcgtcggca attggcggcg gctcgatgag
     4381 cgctggggca agctcattgc gtcctggggc tatgtcacgc tcacggtcga cagtctcggt
     4441 ccacgcggcc ttcaaagcac ctgcaccggc gctccctccg atgatttcgc ttttgacgcc
     4501 taccgcgcgc tgaaattcct ggcgcagcgc tcattcgtcg atcccgctcg cgtcgccgca
     4561 ctgggttttt cgtcgctggg cggctggatc gtgctctcat cggtcgaacg tggcacgatc
     4621 gaacgaaact cgcaggacaa gtttcgtgcc gccgtcgctt tctatccgcc ctgcctggga
     4681 ttcaagggcg acatgaccgt gccgaccctg atcatgatcg gcgagctcga cgattggacg
     4741 acagccacgg cgtgccgcaa catggttgat ggcaaggacg attggggcat atcgcggcag
     4801 aagggcaacg gcgttccggt ccggctgatc gtctatcccg gcgcctatca tgggttcgac
     4861 gcgcccaacc tcaagacacc gagcgatatg ttcggacacc atctggagtt caaccaggcg
     4921 gcagcggacc gatcgatcgc ggcgctccgc gaatttcttg actggacaat cggcgcgagg
     4981 tcgcaaggcc aggcgctggc caagtgacga tcttcaaaca gattcacctc gcgacgaagt
     5041 gcgtatcatg accatcaaag ccgttgtgtt cgacgcctat ggcacgcttt acgacatcca
     5101 gtcggtggcc gagatcaccg aggacgcctt tcccggctat ggcgagatca tcacccagat
     5161 ctggcgcatc aagcagctcg aatacacctg gctgcgttcg ctgatgcgac gctaccagga
     5221 tttttcgcat atcacgcagg attcgctcgc ctacacgttg cgctgcctcg gactgcagca
     5281 tgatcgcgac acgttcgagc ggatcatgga aaaatatctg catctcgatc tctatccgga
     5341 cgcgctcgcc gcgctcgcgg cgttgaaagg caacaagctt gcgatcctct ccaatggcag
     5401 cccggagatg ctgaacgcgc tggtgaaaaa taccggcctc gatcgcgtgc tcgatgccac
     5461 tctcagcgtc gacccaaaaa aggtcttcaa gcccgcaccc gacgcctaca cgctgatcga
     5521 ggaagaactc ggcgtcgcgc cccgcgaggt actgttcgtc tcgtcaaatc cgtgggatgc
     5581 ctgcggtgcc gaatccttcg gcctcaacgt cgcctggatc gagcgcgtga cgccggggtc
     5641 gatggcgctg ctctgtgtcg agaacgagac ggtcgcgccg ctcaccatgt tcaaggcgtt
     5701 gcggacgcag atggacgaac tcggcgtttt gcccgaccat cgcatccgtt cattgtccga
     5761 tttgccgaaa gtcgtcgcat cttatgagcc ttgattccgt ccgcgccttc ttcgccgaaa
     5821 aagcccccga tatcaccgtc atcgaatcgc agcagagttc ggcgaccgtc gtgcttgcgg
     5881 ccgaggccta cggcgtcgag ccggcgcgca tcgcaaagac gctgtcgctt cgggtcggcg
     5941 agcgcgtggt gctgatcgtc acgtcgggca cctcgcgaat ggacaacagg aaggtgaaag
     6001 cgctgttcgg gggaaagccg aagatgctcg ggctggaaga agtcgccgac atcacgggcc
     6061 acgaagtcgg cggcgtctgc ccgtttggcc tgaaggcgcc gctgccgatt tattgcgacg
     6121 tgtcgctcaa agctttcgac atcgtggtgc cggccgcggg ctcaacccac agcgcggtcc
     6181 ggatcgcgcc ggcgcggatg gccgagctga cgtcagccga atgggtcgat gtctgccaga
     6241 acggcgacta gccgctcaaa ctctattctg cgcgcagtgc tcgaggttgg aaccaggact
     6301 ggttccgcag cgttgtaggt tttcccacgc cttgcttacg gaagatgttt tccagcgaca
     6361 cgatgacaat caccgctcaa aaaatattgc cgcgcgccca caccggtatc ccgggactcg
     6421 acgacatcct cgagggcggg ctcgccagaa accgccttca ccttctggaa ggaacccctg
     6481 gcacaggaaa gaccaccatc gccctgcaat ttcttcttgc cggcgcgtcg gtcggcgaga
     6541 ccggcctcta tgtcagcctt gcggaaaccg aagaggaact ccgggaggga gccgcttcgc
     6601 acggctggcg catcgacgag cgggtcagca tctgcgagct ggtgcggccg gaaagcgtgc
     6661 tcgatcccga tcagcatcaa agtctgctct attcgtccga cctcgaactc ggcgaaaccg
     6721 tcgagcgcat gctcgaggcg atcgaaaagt acaagccgca acgcatcgtc atcgacagtc
     6781 tttcggaaat ccggctgctg gcgcagagtt cgctgcgcta ccggcgacag atcctcgccc
     6841 tgaaacacta tttcgcacgc cacaagtcca cggtgatcac gctcgacgac ctcaccaccg
     6901 aaagcacgga cggggccgtt cactcgatcg cacacagcgt gatccatctc gaccagctcg
     6961 cgcccgtgta cggcgccgag cggcggcggc tgcgcgtcac gaagtgccgc ggccagagct
     7021 ttcgtggcgg ttatcacgac ttcacgattg ctacccgcgg cgtgcaggtc tttccgcgac
     7081 tggtggcggc ggagcatcgg catgcatttg cagggacggc gatcaagagc gggatagcgg
     7141 aactggactc gctgctcggc ggcgggctca cggcgggttc gagcacactg atcattggac
     7201 ccgcaggcac cggaaaatcg ctgctctctt tgcagtttct ggcctcgagc gtcaaacgcg
     7261 gcgagcgcgc cggtcttttt gtctttgacg aggagcttgg cctgttgttt tcgcgcgcaa
     7321 aaagcctcgg catcgatatc gaagcgatga gcaaggacgg gaagctgttc gtgcaacagc
     7381 tcgacgcggc cgagctctcg cccggcgaat tcacccaccg cgtccgcagt tgcgtggacg
     7441 agcagaacat ccgcacggtc gccatcgaca gtctcaacgg ctaccaggct gcgatgccgg
     7501 aggaacaatt cctcattctg cacctgcatg agctgttgca atatctcaat cgccgcggcg
     7561 ccgcgacgct tctgacagtc gcccagcacg gcatgatggg cgacatgaag caaccgatcg
     7621 acgtgaccta tctcgccgat accgtggtca tgctgcgcaa tttcgaggca atgggccggg
     7681 tccgccgggc gatctcggtg atcaagaaac gcacgggctc gcacgaggac accatccgcg
     7741 aattgaagat caacggaaat ggtatcaacc ttggtgcgcc tctcgtggaa ttccagggcg
     7801 tgctgtgcgg cgttcccacc tatgtcggca gaagagctcc gctgatggag agttcgtgac
     7861 caaggcagat cattgggaag cggttgagcg gaccttgatc ctggcaccgc gcggacggga
     7921 cgccgcgctc gcggagagca ttctgcgcga ggcgcatctg cgaacggaga tttgcaagtc
     7981 gtttcaggag ctctttgcgg gaataaaggc gggttgcgag ctcgccatcg taaccgagga
     8041 agccttccgt agcgtcgata cgcgcaacct ctcggaatgg gtcagccgac aaccggcctg
     8101 gtcggatttc ccctttatcg tgctgaccga gcatggcggt ggcctcgagc gcaacccgct
     8161 ggcagcgcaa ctcacggagg ccctgggcag cgtcagtttt ctggagcgcc cgtttcaccc
     8221 gacgacgctg atcagcgtgg ttcgaaccca tcttcgcggc cggcgacggc aatacgaatg
     8281 ccgaaagctc aacgaggaac tcgaagtccg cgtccaggag cgcacgcacg aactcgcagc
     8341 cgccaatcgc cagcttcttg gacaaatgga agagcgcgag cgcgtcgaga gcacgctccg
     8401 ccagatgcag cggcttgagg ccgtcggaca gttgacctcc ggcgtcgcgc acgacttcaa
     8461 caatctgctc accgtggtgc tcggcaacat cgctttcatg gagcgggatt ttgcggcacg
     8521 cggcatcgag ggaaagctgg ctcagcggct ccgcttcatg cgcacggcgg ccgaacgcgg
     8581 cgcgaaactg accgaccagt tgctttcctt ttcccggcgc cagcggctcg agccgaaggt
     8641 gctcgatctc aatcacacca tcgcgagcat gcgcgatctc ctgcagagca cgatgggcgg
     8701 catccggatc gagacgtcgc tcgccgacgg tatctggcac gcg
//
LOCUS       DATLJT010000455         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2554241,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000455 DATLJT010000000
VERSION     DATLJT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2554241"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VM910_10485"
     CDS             <1..293
                     /locus_tag="VM910_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62994.1"
                     /translation="EVPVAHRPAATLSNVKGKRDAPSLPANVTCLHSTQTNRSEPTMC
                     VSGMPGAQSCKCSCEVTFSQPKHSFVIMVATPFWNSGEINLGDVIVDKGVRI"
     gene            complement(519..950)
                     /locus_tag="VM910_10490"
     CDS             complement(519..950)
                     /locus_tag="VM910_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62995.1"
                     /translation="MCDYSLHHVATRPAQIEDKLVTTKFSNSITRGFAAIGEPHVAVC
                     LLPGTEIAFDENVECEPSFGVGILANKKIGQRLARFRQINMHNPVTHHDALEFPDGQV
                     VLLSRLCEGQRATVLQLPAAARPEAAKERATPEVREPSLSS"
     gene            1839..2426
                     /locus_tag="VM910_10495"
     CDS             1839..2426
                     /locus_tag="VM910_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HVI62996.1"
                     /translation="MDLFVVPTIGFNLLCVLVIVRLARRELVWINVTAYPTAEWIARQ
                     ITEAFPWNEAPRYLIRDRDGMYGVAVTRRLRAMGIRDKPIASGSPWQNSFAERLIGTA
                     RRECVDHLVVLGETHLRRILREYARYYNEVRTHVSLGKDAPCRRPVERTDGAKFGASY
                     SRARPTRSAQDPAAEVTSIQDTRSVRSSNQPRSVA"
     gene            complement(2641..>2828)
                     /locus_tag="VM910_10500"
     CDS             complement(2641..>2828)
                     /locus_tag="VM910_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI62997.1"
                     /translation="ATAASIDQRSMAAIASPAAWRSIALAGAEAERSKRTRAEVRLVV
                     VSALAAGEEGVWHLMRA"
BASE COUNT          554 a          849 c          868 g          557 t
ORIGIN      
        1 ttgaggttcc agtggctcat cgtcccgccg ccactttgtc gaacgtgaaa ggaaaacgcg
       61 acgcgccatc gctgccggcg aacgtgacat gcttgcacag cacacagacg aatcgttccg
      121 agccgaccat gtgcgtttcc ggaatgccgg gtgcgcagtc gtgcaagtgc tcatgcgaag
      181 tgactttttc gcaaccgaag cattcttttg tgatcatggt cgccacccct ttctggaaca
      241 gcggcgagat caatctcggc gacgtaatcg ttgacaaagg cgttcgaata tgagtctcgg
      301 cttggatttt ttgatgcctt cttccatccg tcacggcctc tatggtccac tttatgaatc
      361 cggcaactgg caaagtcctc tcgggggatg acgattaggg cgctcgcctc agttgcaacg
      421 ccgaattgaa agagccaccg gccgtgccgg tggctcttgg tcttaggcgc atcgatgttt
      481 gccgatgagc acgggtgtgc gccgacgcgc gggcaccatc aggacgaaag cgaaggctcg
      541 cgaacctctg gcgtcgcccg ctccttcgca gcttcggggc gggcggcggc gggcaactgc
      601 agcacggtgg cgcgctggcc ctcgcataga cgcgagagca gcaccacctg cccgtccgga
      661 aactccaacg catcgtgatg ggtcacgggg ttgtgcatgt tgatttgccg gaagcgcgcg
      721 agccgctgtc cgattttctt gttcgcgaga ataccgacgc cgaacgacgg ctcgcattcg
      781 acattctcgt cgaatgcaat ctcggtgccc ggcagcagac agaccgcaac atgcggctcc
      841 ccgatcgcgg cgaagccgcg agtgatggaa ttgctgaact tggtcgtcac cagcttgtcc
      901 tcgatctggg caggccgggt cgcaacatgg tgcaaactat agtcacacat cgaagggact
      961 cctcttcctg tgaagcggga ttctccggat ggctcccgaa ctgacgtgaa tggtagcgaa
     1021 tttctggatt tctcggaagc tcttactcgc tggaccagcc gacgacccgg gtcgcagacc
     1081 gcggccggcg ttgccgccag ccacgtcgct taaatcgtga gttttaggtg gggatgtagt
     1141 agatacgccc gcgtccttcg acattttaaa tttagcgggg tcggcgccaa cattcacgcg
     1201 aacagctgct attttgcgcg agtgtaacgc ggctcacaca tccctgccgc ttcctcccct
     1261 agagtccggt aatgcaggaa cgatgtcaat ttggcatcag gaggaagaca tggcactccg
     1321 actaacgagg ttcgcgcttg tcttcggaat ggtccttgcg acggcggtga tcatccgaac
     1381 gatcacgacg ggggcggccc ccccagcttc tcagagcgag tcgacggcgt caattgcgcc
     1441 gaagcaaccg atctaccggc ctcaactctg gaatgtgttc tgagattcag caagcggacg
     1501 cgcatgcgac agagactggt ttgcaggaat tagggaggtg ctggcgacaa cccggctttc
     1561 cccctggaga ggtgcgccgt cggtgcttcg ggtagagttg acgccgacta aggacttgga
     1621 agaccgagca tccggtactg acgcgaccac tcggctgggg catcgcagag acgggcaact
     1681 ccgatgccgc gcggcgcaag acggtctcgg gcctgacgat cgttggcatc gaacgggttt
     1741 tgctgcttac tggcgatgga agttgagtgt cgtgatcaag gtctgaccat ccgcgctggg
     1801 gcaccttcct gcgcaaccat ctgccgcaga tcgcagccat ggacctgttt gtggtcccga
     1861 ccatcggctt caacctactc tgcgtcttgg tcattgtccg gctggctcgg cgcgagcttg
     1921 tctggatcaa cgtgaccgca tacccgaccg ccgaatggat cgcgcggcag attaccgaag
     1981 ccttcccctg gaatgaggct ccacgttacc tgatccgcga tcgagacggg atgtacggtg
     2041 ttgcggtcac gcgccgatta cgagccatgg gcatccgcga caaacccatt gcatcgggct
     2101 cgccgtggca gaattcgttt gccgagcggt tgatcggaac ggcccgtcgc gagtgcgtgg
     2161 accacctcgt cgtgctgggc gagacgcatc tgcgtcggat cctgcgagaa tatgctcgct
     2221 attacaacga ggttcggacc cacgtctccc tcgggaagga tgcgccctgc agacggcccg
     2281 tcgaacggac cgatggtgcg aaatttgggg cttcctattc gagggcgagg ccgacgcgct
     2341 cggcccagga tcccgctgcg gaggtaactt ccatccagga cacccggagt gtcaggtcat
     2401 cgaaccaacc tcgctcagtg gcttagaagt tcttcaaggc cgacggcatg agggcctaca
     2461 acatatttgc taggcaacaa tcaggtcagc taccgagcca aattttcacc ggaggccgca
     2521 gaagaaaggc cccggcgagg ttgaccaacc ggggcgttag cgtacccagg aaacttagag
     2581 ggtccagcct ggggccgccg ccgcagccag taagcgttcc ttggggcaat tacatgacag
     2641 ctaagcgcgc attaagtgcc acacgccctc ttcgcccgcc gcgagagccg aaacgacgac
     2701 gagccgcacc tccgcgcggg tgcgttttga gcgctccgcc tcagcgccag cgagcgcgat
     2761 ggatcgccac gcggcgggcg acgcgatagc cgccatagag cgctggtcga tagacgccgc
     2821 cgtagccg
//
LOCUS       DATLJT010000456         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1175838,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000456 DATLJT010000000
VERSION     DATLJT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175838"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VM910_10505"
     CDS             <1..135
                     /locus_tag="VM910_10505"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI62998.1"
                     /translation="YRIHGSNEPDTIGQAVSSGCIRLTNEDVIDLYSQVRIGTPVIVQ
                     "
     gene            complement(196..561)
                     /locus_tag="VM910_10510"
     CDS             complement(196..561)
                     /locus_tag="VM910_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HVI62999.1"
                     /translation="MSDAASTPWPTELRLHKDRKALTIAFDNGETFDLSAEYLRVKSP
                     SAEVKGHSPDERKTVAGKKGVAILEVNPIGNYAVRLVFDDMHSTGIYSWEYFLALGRN
                     QDAYWQDYLDDLAGKGLTR"
     gene            1006..>2159
                     /locus_tag="VM910_10515"
     CDS             1006..>2159
                     /locus_tag="VM910_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI63000.1"
                     /translation="MDMIGGELGFMAASAAKAFLTLMEPHRLMFLGLGCIMGLVLGII
                     PGIGGLTGTAMLLPFTFNMDPYAAFALLLGLGATTATGDPIPAILFGVPGGAASAATV
                     LDGFPMAKKGEAGRALSAAYMSSLMGGVFGALLMAISIPILRPVMLFIGSPELLAFSV
                     LGISMVAVLSGNAPLRGITAGCLGVMIAMIGTDPQTGTLRWTFDSLYLWDGLPLTPLL
                     LGVFALPELCDLLIARVAISADTGTANIYKGQWQGVKDCFKHWWLIMRCSWIGGGIGS
                     IPGISASVVDWLAYGHALKTEKGASATFGKGDVRGVIASESSNNAKEGGALVPTVAFG
                     VPGSATMAILLGAFLIHGLVPGPDMLTKNLSITYAMVWSVALANILGAGL"
BASE COUNT          357 a          689 c          702 g          411 t
ORIGIN      
        1 tatcgcattc acggctcgaa cgagccggac acgatcggcc aggccgtgtc gtcgggctgc
       61 atccgcttga ccaacgagga cgtgatcgat ctctacagcc aggtccggat cggcacaccc
      121 gtgatcgtgc aatagccgac gcgggacatc ggatagcgca cggacggcgc agacgcactg
      181 cgttctccgc gcccctcagc gcgtcaagcc ctttccggcg aggtcgtcga gatagtcctg
      241 ccaataggcg tcctggtttc gaccgagcgc gagaaaatac tcccagctgt agatgccggt
      301 cgaatgcatg tcgtcgaaaa cgagccgcac cgcgtaattg ccgatcggat tgacctcgag
      361 aatggcgacg cccttcttgc ccgcgacggt cttgcgctcg tcgggtgaat ggcccttaac
      421 ctcggcgctc gggctcttga cgcgtaaata ttcggcgctg aggtcgaacg tctcgccgtt
      481 atcgaaggcg atggtcagcg ccttgcggtc cttgtgcaac cgcaactccg tcggccaggg
      541 cgtcgacgct gcgtcgctca ttttcgatct cctcgctcgg ggcagatcgg cgtgaaagta
      601 gatcaggcag gcgccgcgcg ataggtcgcg ccgaggttga ggccgagatg gggagcgccc
      661 aaacggcgga gcatctgcgc ccgcaatgtc gctgccccag tataaagtgc cgcagtcggg
      721 gaaatccgac tgcgctgcca aacggctttt ccctcacctg atcttctgct tacatgacaa
      781 caaaataacc accagacggc catgcgggac ttgaggccgg tcgtcgcaaa cgaacgcgaa
      841 gaggggcgac agtcaaatgc gaactcgacc cgacggggtg caggctttca tttcgatgtc
      901 ttgatctggc cctgcaaggc cgaatgcagg gtcgagcgcg cctgtgcgaa acgcatgcgt
      961 cgcaggcgta cgcgcgcgtg acgaatctcg cggagggcgc aagcgatgga catgatcggg
     1021 ggggaactcg gcttcatggc cgcctccgcg gccaaggcct tcctgacgct gatggagccc
     1081 caccggctga tgtttctggg gctcggctgc atcatggggc tcgtgctcgg gatcattccc
     1141 ggcatcggcg gcctcaccgg aacggcgatg ctgctgccgt tcacgttcaa catggatcct
     1201 tatgcggcgt tcgcgctgct gctcggcctg ggcgcgacga ccgcgaccgg cgaccccatc
     1261 ccagcgatcc tgttcggcgt gcccggaggt gccgcctccg cggcaacggt gctcgacggc
     1321 ttcccgatgg cgaagaaggg cgaggccgga cgcgcgctca gcgccgccta catgtcctcg
     1381 ctcatgggcg gcgtcttcgg tgcgctgctg atggccatca gcattccgat cctgcgcccg
     1441 gtgatgctgt ttatcggttc gccggagctc ttggcctttt ccgtgctcgg catttccatg
     1501 gttgcggtcc tgtccggcaa tgcgccgctg cgcgggatca cggccggctg cctcggcgtc
     1561 atgattgcga tgatcgggac ggatccgcaa acgggcacgc tgcgctggac cttcgatagc
     1621 ctttatctct gggacggctt gccgctcacg ccgctgctgc tcggcgtctt tgcgctgccg
     1681 gagctgtgcg acctcttgat cgcccgggtc gcgatctcgg ccgacaccgg gacggccaac
     1741 atctacaagg gccaatggca gggcgtgaag gactgcttca agcattggtg gctcatcatg
     1801 cgatgttcgt ggatcggggg cggcatcggc tccattcccg gcatcagcgc gtccgtcgtc
     1861 gactggctcg cctacggcca tgcgctcaag accgagaagg gcgccagcgc gacattcggc
     1921 aagggcgacg tgcggggcgt gatcgcatcg gaaagctcca acaacgccaa ggagggcggc
     1981 gcgctcgttc ccaccgtcgc cttcggcgtt cccggtagcg ccaccatggc gatcctgctc
     2041 ggtgcgttcc tgatccacgg tctggtgccg ggccccgaca tgctgaccaa gaatctcagc
     2101 atcacctacg cgatggtgtg gtcggtggcg ttggccaaca ttttgggcgc gggcctgtg
//
LOCUS       DATLJT010000457         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1864382,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000457 DATLJT010000000
VERSION     DATLJT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864382"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VM910_10520"
     CDS             complement(<1..358)
                     /locus_tag="VM910_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI63001.1"
                     /translation="MHDGGARALAELRAAGQIGGFGAGINELGMIPRFLDLIDVDFFL
                     VALPYTLLDQQVLDSEFPRCAKKGVGFVIGAVFGSGILATGAVQGARYAYAPAAPEML
                     AKVARIEAACTRHGVPL"
     gene            complement(252..605)
                     /locus_tag="VM910_10525"
     CDS             complement(252..605)
                     /locus_tag="VM910_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI63002.1"
                     /translation="METPPPRDTSLSTPLPAVVEAVAPVPVIAAGGIASGRGLAAVLS
                     LGAQAVSLGTRFLCSDEAFAMQTYKERIVRSTADAADRVARRRRAGSGGAQGGRANRR
                     VRRRHQRARHDPALP"
     gene            569..766
                     /locus_tag="VM910_10530"
     CDS             569..766
                     /locus_tag="VM910_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63003.1"
                     /translation="MTARCLAAAAFQFPLGHPSVASVIPGGLSPEHVRQNVAAFRHTI
                     PSALWAELKHEGLLRADAPTP"
     gene            complement(1434..2000)
                     /locus_tag="VM910_10535"
     CDS             complement(1434..2000)
                     /locus_tag="VM910_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI63004.1"
                     /translation="MTPDQALKELMDGNRRFVEQPMTAFNDDLKLLKEKTADSQAPFA
                     GLLSCADSRVPVELIFDQTIGKLFVTRVAGNIATADLIASLEYGAAVLGTKAIMVLGH
                     SNCGAVKATIDGKAVPGQISTLYRSIRPAVDQAGPNLEATIKANAKVQAGLLRSSSPV
                     LAELIGKGRLKIIAAYYDLARGDVTMLE"
BASE COUNT          489 a          863 c          867 g          518 t
ORIGIN      
        1 cgagcggcac gccatggcgc gtgcacgccg cctcgatgcg cgccaccttc gccagcatct
       61 ccggcgctgc gggcgcgtag gcgtagcggg caccttgcac cgcgccggtc gcgaggatgc
      121 cggagccgaa caccgcgccg atgacgaagc ccactccctt cttggcgcag cgcggaaatt
      181 cgctgtccag cacctgctgg tcgagcaatg tgtagggcaa cgcgacgagg aagaagtcca
      241 cgtcgatcag gtcaaggaag cgcgggatca tgccgagctc gttgatgccg gcgccgaacc
      301 cgccgatttg cccggccgcc ctgagctccg ccagagcccg cgcgccgccg tcgtgcaact
      361 cgatcagcag cgtcggccgt gctgcgtacg attcgctcct tgtaggtctg catagcgaag
      421 gcttcatcgc tgcacagaaa acgcgtgccc agcgagactg cctgggcgcc aaggcttaac
      481 acagcggcga gaccacggcc gctggcgatg ccgccggcgg cgatgactgg aaccggcgct
      541 actgcttcga ctactgccgg cagtggtgtt gacagcgagg tgtctcgcgg cggcggcgtt
      601 tcaattcccg ttgggccatc cctcggtcgc ctcggtgatt cccggcggct tgtcgcccga
      661 gcacgtgagg cagaacgttg ccgcattccg tcacacgatc ccgtcggcgc tctgggcgga
      721 gctgaaacac gagggcctgc tgcgggccga cgcaccgacg ccgtagcgcg tggacgcagt
      781 aagggccgga gccgggtgtc ggtttcggcc tcaaacctgc tgtgcccagc cgtcgtgatg
      841 aaatagttga ccacgtgatt gccgacatcg tgcaaggcgc taggtgggca gtggtttcgt
      901 gggctcgtcg gagttggggc cgatctaccg gagcaccggt cagccgagca gttgccggtc
      961 tcggcacacc aatcgagttg tcaggcggcg gtccgcggag ccgccactca ccgcccggat
     1021 caggcggcgt cacaccaccg ccgcgctgtc ggcggtgcaa gcgcatcgcg gagccgacgg
     1081 ttcgaatccg ttcccctcca gcagagagtc tgcaaacttt cagttcctcg cgcgctgcga
     1141 ctgcgatggc gaaaccagat tcattgcact cgcgtacggg aagttcgaat ccagttccct
     1201 ccagcggcga gtcagatgaa ctctcacccg aacaggcgcg gtcccccgtt ggatctcgag
     1261 gttgtccgct attgggatct cgcggcggcg accgagagga ccaagctcaa cgaccggaca
     1321 agacggtcgg caccaagcac gcccgcgaca agaacggtgg ctaaccccgg ggcggggtgg
     1381 cacctctggt cttagcgaga gggcgggtgt gtcctcgaca ggggatagtt tgcttattcg
     1441 agcatcgtta catcgccgcg tgcgagatca taataggcgg cgataatctt caaccgaccc
     1501 ttgccgatca gctcggccaa caccggcgag gaactgcgca acagtccggc ttggaccttg
     1561 gcgttggcct tgatcgttgc ctccaggttt ggccctgcct ggtccaccgc ggggcggatt
     1621 gagcggtaaa gcgtgctgat ctgcccgggc acggccttgc cgtcgattgt agctttaaca
     1681 gcgccgcaat tggagtgacc gagcaccatg atcgccttgg tgccaagcac cgcggcgccg
     1741 tattcgaggc tggcgattag atctgcggtg gcgatgttgc cggcaactcg cgtgacgaac
     1801 agcttgccga tcgtctggtc gaaaatcagc tcaaccggca cccgggaatc ggcgcaagag
     1861 aggagcccgg caaacggcgc ctgactatct gcggtcttct ccttcaacag cttcaaatcg
     1921 tcattgaacg ccgtcatggg ttgctcaaca aagcgcctgt tgccgtccat cagttccttg
     1981 agtgcctgat cgggtgtcat gtcattttgg gcccacgccg cggtgggcag ggtgactgtc
     2041 gcgcggaaac cggccaaaag gcatgccgtc gctgtgaggc ctaaccgtac gagccgtcgc
     2101 ctcgaccaag agagacacgc agcgtcagct tcgtgaaccg caccgcatcc actggagcgt
     2161 ttggtcgata tcggacaacg aactcgttcg tccgtctggt aatcgggcgc ggctttagcc
     2221 gtaatcatca cactcctccc tcccgggtcg ggttttgctc gccgattagt atgtgtcgcc
     2281 gctacatcat gcggagctgc cgacgcaacc gatttttctt cctcgcgagt cccgggtctg
     2341 ctcactgcag tttcgagcga gcgccgaaag gtagcgatgg gtcgaattcg acggttccac
     2401 gccgatcacg cggatggccg ctctcggcgc atctttctct ttgcagcgcc ttccagggaa
     2461 cgtctcctaa ccgagcccga ggccaccgct cagccttggc ggcgggaact agtgctcatg
     2521 ccccatcgcc gacctttgcc gaccgtcggg aaaccggctg agttgggtgt caagcatgaa
     2581 ctgagggatt ggctgaaggc ctccgtatgt agctcgacga aagcgccagc tagatggtgg
     2641 ccgtcgtgtc tttgaggcag actgctttgt tcgaccgcct gccatagagc tgtcgaatgt
     2701 caaaaattcg tcccgaatag tagttcctcg gtgagac
//
LOCUS       DATLJT010000458         5312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_486265,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000458 DATLJT010000000
VERSION     DATLJT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486265"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1231
                     /locus_tag="VM910_10540"
     CDS             92..1231
                     /locus_tag="VM910_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63005.1"
                     /translation="MKRWRVGLALMGVAVSAAAFAALLPSAREAGAILFSQDDPGALS
                     DLRLSSALRNNQSVVADNIEAALKGGDADLANSFVELARNKDIAVTDDASRRVADAVA
                     EENSSTHFAKRFATGLVTGSADDVASLSGTVAGDLFVFGDIRDVLREGTRFALGEETD
                     HLVLGLAAAGLAVTAATYVSVGGAGPVRAGLTVVKDARKVGRLGEGLAEWAGRSAREL
                     VDTPALEQAVASSSVLRPADSVSAIRAAFRAEKAGALVRLAKDVGRITEKAGARGALD
                     SLKIAEGPKDVARAVRLAEAKGGQTRAILKIFGRGALLLATGAFDLALWLFWAVLALF
                     GLLSSIKSTTERATQAWLDRRKASRLRAQLAAATQSAVAVPAVRG"
     gene            1341..2093
                     /locus_tag="VM910_10545"
     CDS             1341..2093
                     /locus_tag="VM910_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI63006.1"
                     /translation="MPSFHNGDVEIAFLDEGEGDPIVLVHGFASSKNVNWVYPAWVSE
                     LRKNGRRVIALDNRGHGDSAKLYEPGQYSIPMMAGDVVALMDHLDIARADVMGYSLGG
                     RMTAWLGLNRPERLRSAILGGIGAAMIEGGGPGENVVRALEAPSLEDVSDPVGRTFRA
                     FADQTRSDRKALAACLRGSRGLMSRQDAARIAVPVLIAVGTRDEVAGSAHALQEAIPG
                     SEVLDIPDRDHMRTVGDKVYKAGVEEFLSRRM"
     gene            2368..3195
                     /gene="cysE"
                     /locus_tag="VM910_10550"
     CDS             2368..3195
                     /gene="cysE"
                     /locus_tag="VM910_10550"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HVI63007.1"
                     /translation="MAMHQINPQAGKLAALDPIWDRIRAEAEDIVHREPELATFIYST
                     VLHHNRLEDSVVHRLADRLDHSALSGDLIRQTYDEALREVPDLGNVFRADLVAVYDRD
                     PATSRFIDPLLYFKGFHALQTHRLAHWLYLKGRKDFAYYLQSRSSAVFQTDINPAARI
                     GRGIFLDHATGFVCGETAVIEDDVSILHGVTLGGTGKENEDRHPKIRHGVMIGAGAKI
                     LGNIEVGHCARIAAGSVVVKPVPHNVTVAGVPARIVGEAGCAEPSRTMDQMINAMGL"
     gene            3432..3650
                     /locus_tag="VM910_10555"
     CDS             3432..3650
                     /locus_tag="VM910_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HVI63008.1"
                     /translation="MDVQEVRKLDAYLKRVFGNPKIRVVPRPKKDDSAEVYIGEEFIG
                     VLFVDDEDDDRAFQFQMAILEDDLVEQG"
     gene            complement(3658..4359)
                     /locus_tag="VM910_10560"
     CDS             complement(3658..4359)
                     /locus_tag="VM910_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63009.1"
                     /translation="MTMFSTVRFTSRGWRDGHLLPIALATALTAGAVAIVAYLLWPTW
                     STDGSNAPDRLPVSIGGTLFNVPAAAIRMKIQRHSGPQERVDLAFGFPSLEPPESPKH
                     VSADAVDEQVQPIDRIFLSIAAHHDTLAPDMRVRTIYPRYLAGGSTPLENGLTVRTFR
                     EGTPYDGEDLFYANTPGITARCTRDAATPGMCLSERRIDGADLTFRCPRSWLSEWRDV
                     ASAMDRLTAQLRGTK"
     gene            4625..4954
                     /locus_tag="VM910_10565"
     CDS             4625..4954
                     /locus_tag="VM910_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63010.1"
                     /translation="MSPDAVNSLFALCIGFALAGAFASGYQAFAQRPAGFALLQEGVV
                     PHRFAAVPFLVFAAPFIIMRNTLAGVQVESRRFEFVMMATVIAGFWSMMSGTFFLMTL
                     RAAGLLA"
     gene            5056..>5312
                     /locus_tag="VM910_10570"
     CDS             5056..>5312
                     /locus_tag="VM910_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HVI63011.1"
                     /translation="MAIYELDGQGPDLPADGNYFIADTATVIGKVRLKQGASVWFGAV
                     LRGDNEWIEIGEGSNVQDGSTCHTDLGFPLTIGKNCTVGHNA"
BASE COUNT          925 a         1610 c         1740 g         1037 t
ORIGIN      
        1 tcgctccgga gaagcccttc tttcgtcggg gccgttgtgt cgcaaaaccg gcacactatt
       61 ttaaagctgc gcaaagtgcg gggtttttcg aatgaaacga tggcgggtcg gtctggccct
      121 gatgggggta gcggtcagcg cggcggcgtt cgccgcgctt ttgccgtctg cgcgggaagc
      181 cggcgcaatc ctcttctcgc aagacgatcc gggcgcgctg tctgacctga ggctcagttc
      241 tgccttgcgg aataatcaaa gcgtcgtggc cgacaatatc gaagcagcgc tcaagggcgg
      301 cgacgccgat ctcgccaaca gctttgtcga gctggcgcgg aacaaggata tcgcggtgac
      361 ggatgacgcc tcgcgccgcg tcgctgatgc ggtggcggag gagaactcgt cgacgcattt
      421 tgcaaaacgc tttgccaccg gcctcgtcac cggcagcgcc gacgatgtcg caagcctttc
      481 gggaaccgtt gcgggcgatc tcttcgtatt tggcgacatc cgcgatgtgc tgcgggaagg
      541 aacccgtttc gcgctcggcg aggaaaccga ccatctggtg ctgggccttg cggccgcagg
      601 ccttgcggtg acggcggcga cctacgtgtc ggtcggcggc gcgggacctg tgcgcgccgg
      661 actgaccgtg gtcaaggatg cgcgcaaggt cggccgcctc ggtgaggggc ttgcggaatg
      721 ggccggccgc tcggcgcgcg aactcgttga cacgcctgcg ctggagcagg cggttgcgtc
      781 ctcctccgtg ctgcggcccg ccgacagcgt aagcgcgatc cgcgccgctt tccgtgcgga
      841 gaaggccggc gcgctggttc ggcttgccaa ggatgtcgga cgcatcaccg aaaaagcggg
      901 cgcccgcggc gccctcgact ccttgaagat cgccgaaggt ccaaaggatg tcgcccgtgc
      961 ggtgcggctg gcggaggcaa agggcggcca gacgcgcgcg attctgaaga ttttcggtcg
     1021 cggcgcgctg ttgcttgcga ccggtgcgtt cgacctcgcg ctgtggctgt tctgggccgt
     1081 tctggcgctg ttcggtcttc tgtcctcgat caagtccacc accgagcggg ccacccaggc
     1141 ctggctcgac cgcaggaagg caagtcgcct gcgcgcacaa ttggcggcgg cgacgcaatc
     1201 agcagtggcg gtccccgccg tgcgcggcta gtcatcggat tccaacattc gcatcccacc
     1261 tgggaggcag atttgcaaac gtcgaattcg caccactagc gttcgcggtt cattttcact
     1321 acaggggcac gggacattcg atgccgagtt tccacaacgg cgatgtcgaa attgcctttc
     1381 tcgacgaagg tgagggcgat ccaatcgtgc tcgtgcacgg ttttgcgtcg agcaagaatg
     1441 tgaactgggt ctatccggcc tgggtctcgg aactgcgcaa gaatggccgc cgcgtcatcg
     1501 ccctcgacaa tcgcggccat ggcgattcag caaagctcta tgagcccggg caatattcga
     1561 ttccgatgat ggccggagat gtcgtcgcgc tgatggatca tctcgatatc gctcgcgccg
     1621 acgtcatggg ctattcgctc ggcggacgga tgaccgcgtg gctcgggttg aacaggccgg
     1681 agcggctgcg ttcggccatt ctcggcggta tcggcgccgc catgatcgaa gggggcgggc
     1741 ccggcgagaa cgtcgtgcgc gcactcgaag cgccgtcgct cgaggatgtc agcgatcccg
     1801 tgggtcgaac gttccgtgcg ttcgccgacc agacccgatc cgaccgcaag gcgctggccg
     1861 cctgcctgcg cggctcgcgc gggctgatga gcaggcaaga cgcggcccgg atcgccgtgc
     1921 ccgtgctgat cgcggtcggc acgagggacg aggtcgcagg ctccgcgcat gcgcttcaag
     1981 aggccattcc cggatcggaa gtgctcgaca ttcccgaccg cgaccacatg cgcactgtcg
     2041 gggacaaggt ctacaaggcg ggcgttgagg aatttctctc acggcgcatg tgaagcctcc
     2101 tccggctcat tgtcgtcgcc gggagccttg tcctgcacga caggggcgtt gcggccagtg
     2161 caggcggaga atgatattct ctcaggatcg ccatgaaatc agctcatgcg tgctgccgta
     2221 ccgcttgaat ttcctcaatg gctgaccacg tttgctttcc accggcgttt gcgcgagtga
     2281 agcattcgcc tttggcggag gctgttagga ctttgtcgcg ccgtcgtggt ataggatagg
     2341 tagaaagaaa ttctcgggag ccgctgcatg gcgatgcatc aaatcaatcc gcaggctgga
     2401 aagctggcgg cgctcgatcc gatctgggat cgcatccgcg ccgaggccga agacatcgtg
     2461 catcgcgagc cggagcttgc gaccttcatc tactccaccg tgctccatca caatcgcctg
     2521 gaagactcgg tggtgcatcg tctggccgac cgcctcgacc attcggcgtt gtcgggcgac
     2581 ctgatccgcc agacctatga cgaggcgctg cgcgaggttc cagatctcgg caacgtgttc
     2641 cgagccgatc tcgttgcagt ctacgaccgc gatcccgcga cctcgcgctt catcgatccg
     2701 cttctctact tcaagggctt tcatgcgctg cagacgcatc gccttgcgca ctggctctat
     2761 ctgaagggcc gcaaggattt cgcctactac ctgcagagcc gctcctccgc ggtgttccag
     2821 accgacatta atccggccgc caggatcggc cgcggcatct tcctcgacca cgctaccggc
     2881 tttgtctgcg gcgagaccgc ggtcatcgag gacgacgtct ccattcttca tggcgtcacg
     2941 ctcggcggga ccggcaagga gaacgaggat cgtcatccca agatccgcca tggcgtcatg
     3001 atcggcgccg gggcaaagat cctcggaaat atcgaggttg gccattgtgc gcggatcgcg
     3061 gccgggtccg tggtggtgaa gccggttccg cacaatgtga cggtcgcggg cgttcccgcc
     3121 aggatcgtgg gcgaggccgg ctgtgcagag ccgtcgcgca ccatggacca gatgatcaac
     3181 gcgatggggc tgtaagaaat ccacatcatg atttctgagc aaacgggcgg gcgcaaagcc
     3241 ggccccttcg tcttgcggtg tgtcgtcatg ccccgatacg aatcgaaaat cgcataagaa
     3301 tcattgattt gtgtgcactt ttacgcgtcg acgggcaagt gacctcgctt gctgttttgt
     3361 ttgcagtgga cactagccgt gcgtcgcatc tcgtcctaaa acccaccaac ctcagtgacc
     3421 gggagaccgc cgtggacgtt caggaagtca ggaagctcga cgcctatctc aagcgcgtgt
     3481 tcggcaatcc caagattcgt gtggtgccgc gaccgaagaa ggacgattcc gccgaggtct
     3541 atatcggcga ggagttcatc ggcgtgctgt tcgtcgatga cgaagacgac gaccgcgcgt
     3601 ttcagttcca gatggcgatc ctcgaggacg atctggtcga gcagggctga tcatttgcta
     3661 cttcgtgccg cgtagctgcg ccgtcagtcg gtccatcgcg ctggcgacgt cgcgccattc
     3721 cgacagccaa ctccgcggac agcggaacgt caggtcggcg ccgtcgatgc gtcgctcgct
     3781 caggcacatg ccgggggttg cggcatcgcg cgtgcagcgc gccgtgatgc ctggggtgtt
     3841 ggcgtagaac aggtcttcgc cgtcataggg cgtaccctcg cggaaggtgc gcacggtgag
     3901 cccgttctcg agaggggtcg agccccctgc aagatagcgc ggatagatgg tgcgcacgcg
     3961 catgtccggc gccagcgtgt cgtgatgcgc cgcaatcgac aggaagatac ggtcgatcgg
     4021 ctgcacctgc tcgtcgaccg catccgcgct gacatgtttt ggcgattccg gaggctccag
     4081 cgacggaaag ccgaaagcga gatccacacg ttcctgcggc ccggagtgcc gctggatctt
     4141 catgcggatc gcggcggcag gcacgttgaa caatgtgccg ccgatcgaga ccggcagccg
     4201 gtcaggcgcg tttgagccgt ccgtgctcca ggtcggccag agcagataag cgacaatcgc
     4261 aacggcgccg gcagtcagcg cggttgccag cgcaatcggt aacagatggc cgtcgcgcca
     4321 accgcgcgaa gtgaagcgga ccgtcgaaaa catcgtcatg aatggcaaac gtgaaatggc
     4381 acccgaatca aagccgacta tgccatgcgg caccgtcttt gaggcgggtt tccggcggcc
     4441 aggggctcca gcggcgatgc agagggcaag ggctgcgccc ggagcattaa cctttcttta
     4501 aggatgatgt ggcggcgggc gggcgatttt gccaaagagg ggcgccgatg agtgccgccg
     4561 atttgaagtc gaaagcggta ctggaatcct ggtttgcgat gccccagttg tgcgggagag
     4621 tgcgatgtca cccgatgcag tgaattcgtt gttcgcgctc tgcattggtt ttgcgctcgc
     4681 cggcgctttc gccagcggct atcaagcctt cgcgcaacgt cccgcgggct ttgccctgtt
     4741 gcaggaaggc gtcgtgccgc atcgctttgc cgctgtgccg tttctggtgt tcgcggcgcc
     4801 cttcatcatc atgcgcaata cgcttgccgg cgtgcaggtc gagagccggc gcttcgagtt
     4861 tgtcatgatg gcaaccgtga tcgcgggctt ctggagcatg atgagcggca ccttcttcct
     4921 gatgacgctg cgtgccgccg gactgctggc ctgaaggctc ggcctcgtct ttcgagccga
     4981 aaaggtttca tctccccctc gcttgaaaat ccgtcgctcc ccgtgccaag gtcgccctga
     5041 gcatgggaga ttttcatggc gatctacgaa ctggacgggc aggggcccga tcttcccgcc
     5101 gacggcaatt attttatcgc cgacaccgcg acagtgatcg gcaaggtgcg gctgaagcaa
     5161 ggcgccagcg tctggttcgg cgcggtgctg cgcggcgaca atgagtggat cgagatcggc
     5221 gagggctcga atgtgcagga cggttcgacc tgccacaccg atctcggttt tccgctcacc
     5281 atcggcaaga attgcaccgt cggccacaac gc
//
LOCUS       DATLJT010000459         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1175991,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000459 DATLJT010000000
VERSION     DATLJT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175991"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VM910_10575"
     CDS             complement(<1..510)
                     /locus_tag="VM910_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI63012.1"
                     /translation="MRILHILDHSLPLQSGYVFRTLGIINQQRALGWEPVLLTSGKHY
                     APGPTRERIGGWEFLRTPMPTGFAARLPWLRELKIVSDLDRRLDDVIGEVRPEILHAH
                     SPALNALSAIRAGRRHRVPVVYEMRSLWEDAAGSGATQGRAGLRYRATKLLETHALRR
                     ADAVTTICQG"
     gene            612..1817
                     /locus_tag="VM910_10580"
     CDS             612..1817
                     /locus_tag="VM910_10580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI63013.1"
                     /translation="MPKQTPYCEWTSVIRDVEFSYESLPPLASLEREWRTLEAVARPS
                     FFTSWQWIGTLLAAVPAAHRPKLLRGAAGGETVGLALLGGGVTRRRHGLVRSRALYLN
                     ETGHPRFDSITIEHNGILAEVGRETAVFDSAIAWFADLGDVADELYISGSLLRLPETA
                     VEARGLGRSETTLPSYSVDLGCLSPSGGELYPVLSANARQQLRRALRHFERRGPLQLS
                     AAATTAEALAFFTELKVLHCASWKRRGKTHSFSGEFFEPFHRLLIERSFTGGGTQLLR
                     ASAGDRIIGYLYNFRLGDRIYAYQSGFADADRRERPGIVTHALAIRHAFQSGARVYDF
                     MAGRNRLKESFSTRCEPMLWQVFQQPRLAFRLEHLGRRFKHAIFARKPLSAVQRSDRA
                     SRRSRPARG"
     gene            1844..>2184
                     /locus_tag="VM910_10585"
     CDS             1844..>2184
                     /locus_tag="VM910_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63014.1"
                     /translation="MSILLTGAAGFIGFHVAKALLERGDRVVGVDNLNDYYDVQLKEA
                     RLALLDAYPGFVFAKLDVADRDSVLALIEAHRDLSSIIHLAAQAGVRYSLENPYAYID
                     ANVMGTLVMLEA"
BASE COUNT          372 a          703 c          675 g          434 t
ORIGIN      
        1 gccctgacag atcgtcgtca ccgcatcagc gcggcgcaga gcgtgtgtct cgagcagttt
       61 tgtcgctcga tagcgcagac cagctctgcc ctgcgtggcg ccgctccccg cagcatcttc
      121 ccagagcgag cgcatttcat aaaccaccgg cactcgatgc cggcggccgg cgcggatcgc
      181 cgacaacgcg ttgagcgcgg gtgagtgggc gtgaaggatc tctggtcgca cctctccgat
      241 gacatcgtcg agtcgccggt ctaggtcgct gacgatcttc agctcgcgaa gccacggcag
      301 cctcgcagca aaaccagttg gcattggggt gcgcaggaat tcccacccgc cgatccgctc
      361 gcgcgtgggc cccggcgcat aatgcttgcc actcgtcagc agcacgggct cccaccccaa
      421 cgcccgctgc tgattgatga tgccgagcgt acgaaacaca tagccgctct gcagcggcag
      481 cgaatggtcg agaatgtgca gaattcgcat cactcacacc ggctcttgtt aatgatcacg
      541 ccgcattatg caaggctcaa gcgggggtcg agtttccgtc aaggcgcctg atatactttt
      601 aaagtggttt cgtgccgaaa cagacgcctt attgcgaatg gacgagcgtg attcgcgacg
      661 tcgagttcag ctatgaatct ctgccgccgc tcgcgagcct tgagcgcgaa tggcgaacgc
      721 tcgaagccgt ggcacggccg tcctttttca cctcgtggca gtggatcggg accttgcttg
      781 ccgcggtgcc ggcggcgcat cggccgaagc tgctgcgcgg agctgcgggg ggcgagacgg
      841 tcgggttggc attgctgggt ggcggcgtca cccgccgccg gcacgggctg gtccggtcac
      901 gcgctcttta cctcaacgag accggccatc cgcgcttcga ttcgatcacg atcgagcaca
      961 acggcatcct tgccgaggtt ggccgcgaaa cggcggtttt cgattcggcg atcgcctggt
     1021 tcgccgatct cggcgacgtg gcggacgaac tctatatcag cggctccctg ctgcgccttc
     1081 ccgagacggc ggtcgaggcc cgcgggctcg gccgaagcga aaccaccctc ccctcctact
     1141 ccgtcgatct cggttgcttg tcgccgagtg gcggcgagct ctacccggtg ctgagcgcca
     1201 atgcccgcca gcagcttcgc cgcgccctcc ggcatttcga gaggcgcggc ccgttgcaac
     1261 tgagcgccgc ggcgacaaca gcggaggcgc tagctttctt cacggaattg aaggtattgc
     1321 attgcgcttc ctggaagcgt cgcggcaaga cacattcgtt ctcgggcgag ttcttcgagc
     1381 cgttccaccg cctgctgata gagcgcagct tcaccggggg cggaacacaa ctacttagag
     1441 cctccgccgg cgaccgcatc atcggctacc tttataattt tcggttggga gaccggatct
     1501 atgcctatca aagcggcttc gccgatgccg atcggcggga gcggcccggc atcgtgaccc
     1561 atgccttggc tattcggcac gcatttcagt cgggtgcccg agtttatgat ttcatggctg
     1621 gacgcaatcg gctcaaagaa agtttttcaa cccggtgcga gccgatgctg tggcaggtct
     1681 ttcagcagcc gcgcctggcc tttcgcctcg aacacctcgg acgtcgcttc aagcacgcga
     1741 ttttcgcgcg aaaacccttg tcggcggtgc agcgaagcga tagggcaagc cgccgctctc
     1801 ggccggctcg gggctgagct cgtcatctag cgccaggagc gtcatgtcga tccttctcac
     1861 cggtgctgcc gggttcatcg gcttccacgt cgccaaggcg ctgctcgagc gcggtgatcg
     1921 ggtcgtgggc gtcgacaatc tcaacgatta ctacgatgtg cagttgaagg aggcgcgcct
     1981 ggccctgctg gacgcctacc cgggctttgt cttcgccaag ctcgatgttg ccgaccgtga
     2041 cagcgtctta gccctcatcg aggctcacag ggacctgagc agcatcatcc atctcgcggc
     2101 gcaggccggc gttcgctact cgcttgagaa cccctacgcc tatatcgacg ccaatgtgat
     2161 ggggaccctg gtaatgctgg aggc
//
LOCUS       DATLJT010000460         4339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3013529,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000460 DATLJT010000000
VERSION     DATLJT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013529"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VM910_10590"
     CDS             complement(<1..297)
                     /locus_tag="VM910_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HVI63015.1"
                     /translation="MITKSKKANPLPDVADLTKAQAKVEHKRLALELEGHDRRYYQED
                     APSVSDAEYDALRRRFNAIEKRFPEFVTSESPSQKIGAAPSGRFRKVRHAVPMLS"
     gene            complement(391..2064)
                     /gene="recN"
                     /locus_tag="VM910_10595"
     CDS             complement(391..2064)
                     /gene="recN"
                     /locus_tag="VM910_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HVI63016.1"
                     /translation="MLARLSIRDIVLIERLDIEFAKGLAVLTGETGAGKSILLDAFAL
                     ALGGRGDAGLVRHGAEQGQVTAVFDVPRDHPATAILSANGLDDTGEMILRRVQFADGR
                     TRAFVNDQTISVQTLKAVGSALVEIHGQHDERALVDAATHRRLLDAFAGLEKDVGALE
                     ALWDARRTANAALEEHRASMERAAREADYLRHASDELKMLAPKDGEETALAERRTAMM
                     AGEKIAADLREAQDAVSGDHSPVAALSAAVRRLERRAANSPALVEPAVKAIDAAINAL
                     EEADQHLTTALAAADFDPAELERIEERLFALRAASRKYSTPVDGLAALAAKYAADVAL
                     IDAGAEQLKRLEAAAGEADQRYASAARKLSAAREKSAQKLDRAVNAELPPLKLERATF
                     TTQIESDPDSAGSQGFDRIEFWVQTNPGTRPGPLMKVASGGELSRFLLALKVVLSDRG
                     SAPTLVFDEIDTGVGGAVADAIGARLARLAGKVQVMAVTHAPQVAVRANQHLLISKDS
                     LDKGKRVATRVNALAADHRREEIARMLAGAEITAEARAAAERLLRAATA"
     gene            complement(2089..2991)
                     /locus_tag="VM910_10600"
     CDS             complement(2089..2991)
                     /locus_tag="VM910_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HVI63017.1"
                     /translation="MSVRHTARKQRSISAGSRAEGRRLWLAVALVALAVPLGGCGTGP
                     LWDKFLAKDDTFVDEPADKLYNEGLYLMNERKDNKAASKKFEEVDRQHPYSDWARKSL
                     LMSAYSYYQSGDYDSCIGAATRYVTLHPGSPDAAYAQYLIAASHYDQIPDVSRDQGRT
                     EKAIAALEEVVRKYPTSEYAVSAKAKMQAARDQIAGKEMDIGRYYMEKRDFTGAINRF
                     KTVVTQYQTTRHVEEALARLTEAYMAIGVVGEAQTAAAVLGHNFPDSRWYKDAYNLVR
                     SGGVEPSENQGSWISRAFKKVGLG"
     gene            complement(3205..4164)
                     /gene="lpxC"
                     /locus_tag="VM910_10605"
     CDS             complement(3205..4164)
                     /gene="lpxC"
                     /locus_tag="VM910_10605"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HVI63018.1"
                     /translation="MKFSRQTTLRSQATVTGIGVHSGLPVSLTLGPASVDAGFVFVRT
                     SLEGRDREVQATAEAVISTEFATVLGDREGPLVATAEHVLAALRGMGVDNATIEIDGP
                     EVPIMDGSAAAFVAAIDQAGVVSQSVARRFIQVVKPVQVAMGDSYGELRPHASGFRAE
                     VEIDFTNPVIGRQTYSLELSPEGFRREICRARTFGCMADVARLWSAGFALGASFENSL
                     VFDESRLLNPEGLRFADECARHKVLDVVGDLALAGLPLLGAYRSLRGGHKLNCAVLTA
                     LMADRSAWRVVEAETVRRSRTQVEAVGGMVGGLVAPAFGPDMS"
BASE COUNT          769 a         1456 c         1332 g          782 t
ORIGIN      
        1 cgacagcatc ggcacggcgt ggcggacttt cctgaaacgc cccgacggcg ccgcgccgat
       61 cttctgcgac ggcgactccg acgtgacaaa ctccggaaag cgtttctcga tcgcgttgaa
      121 gcgccggcgc agcgcgtcgt attcggcgtc cgagacgctc ggcgcgtctt cctggtaata
      181 gcgccggtcg tgtccttcga gctcgagcgc cagacgcttg tgctccacct tggcctgcgc
      241 cttggtcagg tcggcgacat cgggaagcgg atttgctttc tttgattttg ttatcatgat
      301 gcaaagctgt gttcgtcatt ccgggctagt ccgaaggacg ggctcgacgc ttcgcatcgc
      361 cccggactga cattgcaaaa tacgatagaa tcaagccgtt gcggcgcgca agagtcgctc
      421 tgccgctgcg cgggcctccg cggtgatctc ggcgccggcc agcatgcggg caatctcctc
      481 gcggcggtga tcggcggcga gcgcgttgac gcgcgtggcg acccgtttgc ccttgtcgag
      541 cgaatccttg gagatcagaa ggtgctggtt ggcacgcacc gcaacctgcg gcgcatgggt
      601 caccgccatc acctgcacct tccccgcgag ccgcgcaagg cgggcgccga tcgcatccgc
      661 caccgctcca ccgacgccgg tatcgatctc gtcgaacacc agcgtgggtg ccgagccccg
      721 atcggacaag acgaccttca gcgcgagcag gaagcgcgaa agctcgccgc cggaggcgac
      781 cttcatcagc ggccccggcc tcgtgccggg attggtctgg acccagaact cgatgcggtc
      841 aaagccctga gatcccgctg agtcgggatc gctttcgatc tgcgtcgtga acgtggcacg
      901 ctcgagcttc agcggcggga gctccgcatt gaccgccctg tccaactttt gcgcggattt
      961 ttcccgcgcg gcagagagtt tcctggcggc agacgcgtag cgttggtcag cctccccggc
     1021 cgccgcctcc agtctcttca actgctcggc gcccgcgtcg atcagcgcga catcggcggc
     1081 gtattttgcg gcgagcgccg cgagcccgtc caccggtgtc gaatatttgc gcgaggcggc
     1141 acgcagcgcg aacagccgtt cctcgatgcg ttcgagctca gcgggatcga aatcggccgc
     1201 ggcgagcgct gtggtcagat gctggtccgc ctcttccagg gcgttgatcg ccgcatcgat
     1261 cgccttcacc gcgggctcga ccagcgcggg cgagttcgcc gcgcgacgtt ccagccgccg
     1321 caccgccgcc gacagcgccg caaccggcga atgatcgccg ctgactgcgt cctgcgcctc
     1381 gcgcagatcg gccgcgatct tctcgccggc catcatcgcg gtacgccgtt cggcgagcgc
     1441 tgtctcctcg ccgtccttcg gcgcgagcat cttcaactcg tcggaggcgt ggcgcagata
     1501 atccgcctcg cgcgcggcac gctccatgct cgcgcggtgc tcttcgagcg cagcgttggc
     1561 ggtgcggcgc gcatcccaca gcgcctcgag tgcgccgaca tccttctcca ggccggcaaa
     1621 agcatccagc aggcggcgat gcgtcgccgc atcgaccagt gcgcgctcgt catgctggcc
     1681 gtggatctcg accagcgccg aaccgactgc cttcagggtc tgcacgctga tcgtctggtc
     1741 gttgacgaaa gcgcgggtgc ggccgtcggc aaactgtacg cggcgcagga tcatctcgcc
     1801 ggtatcgtcc aggccgttag cggacaggat cgcggtggcc ggatgatccc gcggcacatc
     1861 gaacacggcg gtgacctgcc cctgctccgc gccatggcga acgaggccgg catcgccgcg
     1921 gccgccgagc gccagcgcaa aggcatcgag caggatcgat ttgccggcgc cggtttcgcc
     1981 ggtgagcacc gcgagcccct tggcaaattc gatatcaagc cgctcgatca ggacgatgtc
     2041 gcggatcgac agacgcgcca gcatgcggga gaagttcctg agccagtcct agccgaggcc
     2101 gactttcttg aaggccctgc tgatccagga accctgattc tcgctcggct cgacgccgcc
     2161 ggatcttaca agattatagg cgtccttgta ccagcggctg tcgggaaaat tgtgcccgag
     2221 cacggcggcg gcggtctgcg cttcgccgac cacgccgatt gccatgtagg cttcggtgag
     2281 acgcgccagc gcttcctcga catggcgtgt ggtctggtac tgcgtgacca cggtcttgaa
     2341 gcggttgatg gcaccggtga aatcgcgctt ttccatgtag tagcggccga tatccatttc
     2401 cttgccggcg atttggtcgc gcgcggcctg catcttggcc ttggcactga ccgcatattc
     2461 cgaggtcgga tatttccgga ccacctcttc gagtgcagcg atcgccttct cggtccgacc
     2521 ctggtcgcgc gagacgtcgg gaatctggtc gtaatgcgag gccgcgatca ggtattgcgc
     2581 gtaggcggcg tccggacttc cgggatgcag cgtcacgtag cgggtcgcag cgccaatgca
     2641 actgtcgtag tcgccggact gataatagga ataagcagac atcagcagcg atttgcgcgc
     2701 ccagtcggaa taaggatgct gacggtcgac ctcttcgaat ttcttcgagg ctgccttgtt
     2761 atccttgcgc tcgttcatca ggtacaagcc ctcattgtag agcttgtccg cgggctcatc
     2821 gacgaacgta tcgtccttgg ccaaaaactt gtcccaaagc gggccggtgc cgcaaccgcc
     2881 caacgggacc gcaagcgcaa ccagcgcgac ggcgagccaa agccgccggc cttcagcccg
     2941 gctgccagcc gatatgctgc gttgtttgcg cgctgtatgc cgtaccgaca tgtgccctag
     3001 aacctgacgt cttcagtgcg tttcgtacgg tacccaccgt tgccggtgac ggatgctgcc
     3061 gtggccccag cgccgccaag cgaccgaagc ccgtgtagcc gaaaaacctg cagtaaccaa
     3121 ccgcataggc aggcatttcg cccggattaa ggcggctaaa aggcagtgcg actgattgtg
     3181 cttactgcaa aaggccttgg tcgctcagga catgtcgggg ccaaaggccg gcgccacgag
     3241 gccgcccacc ataccgccca cagcctccac ctgggtgcgg gaccgccgca cggtctcggc
     3301 ttcgaccacg cgccaggcgc tgcgatcggc catcaatgcg gtgagtacgg cgcagttcag
     3361 cttgtgaccg ccacgcagcg agcgataggc gcccagcagc ggcagaccgg cgagtgccag
     3421 atcgcccacc acgtccaaaa ccttgtgccg cgcgcattca tcggcaaacc gcaacccctc
     3481 gggattgagc aggcggctct catcgaacac cagcgagttc tcgaaggagg cgccaagtgc
     3541 aaaaccggcg ctccagagcc gcgcgacgtc ggccatgcag ccgaatgtgc gagcgcggca
     3601 aatctcgcga cggaaccctt ccgggctaag ttcgagcgaa taggtctgcc ggccgatgac
     3661 cggattggtg aaatcgattt ccacctcggc gcggaaaccc gatgcatgtg gacgcagctc
     3721 gccataactg tcgcccatcg cgacctggac gggcttcacg acctggatga agcggcgcgc
     3781 caccgattgg ctgaccacgc ccgcctggtc gatcgccgcg acaaaggccg ctgcgctgcc
     3841 atccatgatc ggaacttcgg gaccgtcgat ctcgatcgtg gcgttgtcga cacccattcc
     3901 ccgaagcgcg gcgaggacat gttccgccgt ggcaaccagc ggaccctcgc ggtcgcccag
     3961 gacggtggca aattctgtgg aaatcacggc ctcggccgtc gcctggacct cgcggtcgcg
     4021 accctcaagg ctcgtacgca caaaaacaaa acccgcatca acagatgcag gtccgagggt
     4081 caaactgaca ggtaacccgg aatgcacgcc gatacccgtc acagtagctt gcgaccgaag
     4141 cgtggtttgc cggctgaact tcatgataga cccgcccact acgactttac ccaaagcaac
     4201 ttcggccccg aaatcccccg gccgattcgt gcttttaggt atccccaagt cgccgcaaac
     4261 ataatcacag ccccaaagag cgccaactca cgctttttta ccgattgtta ccctatctct
     4321 gccgtattgg cgcccgtcc
//
LOCUS       DATLJT010000461         9134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3243749,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000461 DATLJT010000000
VERSION     DATLJT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243749"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VM910_10610"
     CDS             complement(<1..1280)
                     /locus_tag="VM910_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI63019.1"
                     /translation="MSLKGANSPTRGRKLRSTGTKAAARVSNGPYSLVELKKQLEVRT
                     RELAEARGHLSEALEQQAATSEVLQVISTSPGELEPVFQAMLANAVRICEAKFGTLYL
                     HERGALRLVASHNVPAAFAEARRRGPFHPPPGGPLNEVIRTKHTVQTADMAASQAYAE
                     RHPVTVDAVELGGVRTAVSVPMLKDDELIGVIAIFRQEIRPFTDKQVAMLANFANHAV
                     IAIENTRLLNELRESFQQQTATADVLKVISRSTFDLQTVLDTLVESAARLCEAERANI
                     WRPSGDVYRVAATFALSAEHEQTLKRLAIRPGRDTITGRTLLEGKTVHIPDVLADPEY
                     DSPVLKIGANRTVLGVPLLREGVPIGVLVVTRSTVRPFTDKQIELVTTFADQAVIAIE
                     NVRLFQAEQQRTRELTESLEYQTATGDILRVISSS"
     gene            complement(1453..1887)
                     /locus_tag="VM910_10615"
     CDS             complement(1453..1887)
                     /locus_tag="VM910_10615"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63020.1"
                     /translation="MAHKPYRGGGPALTDLLSGQVQVMFATMPSSIAYIRAGKLRALA
                     VATATRSEALPDVPTVGEFVPGYEASSWSGVGAPRATPAEIVDKLNKEINAGLADPKM
                     KARLADLGGTVLPGPPSDFGKLIAEETEKWAKVVKFAGIKPE"
     gene            complement(2001..2345)
                     /locus_tag="VM910_10620"
     CDS             complement(2001..2345)
                     /locus_tag="VM910_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63021.1"
                     /translation="MKVINEPGLAKVSSTDLLHELTNELVRVRRRLKRARLKFAGLQA
                     LKRDLQPYLQSGDYEHLLELLKREIERREREETELSTRCRDERFRQAVGDERFEKSRR
                     ELAEIHRKLGLD"
     gene            complement(2479..3822)
                     /locus_tag="VM910_10625"
     CDS             complement(2479..3822)
                     /locus_tag="VM910_10625"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HVI63022.1"
                     /translation="MAKSSPAQRFAKIYAVWIHKSTSAGDRANAKRQIDNWLKHYRKT
                     INDIPEILVQAAADDAAQAPSPPPSDPRDAQPHPFEDPKFTPVGLVHGIVGKYLTMTP
                     HVQVIYSLYIVATHVYRQFDIAARLALVSELPGSGKSEALKVAKCLMLRPNPEALSTG
                     AAVIDFIDAGPCTIGLDELDLLDAEARQYILLLWNLGHDRALAKRALMVKGKRKVVDF
                     HAPMMAAGLGSFLADAQMTRTFILEMHKYTAETKPERKFKATDTADLDAVYSFLRHRT
                     ANMKLNLDPPMPPGVIARDADNACSLLAVADACGPEWGQRAREAILFLIEKARTEQPK
                     IRMIRHGLAIFDALGIKQIRSTQFNKELKRLDLPDADWTRYRDLSGLDKAHPLTMNEQ
                     VKLLGMVGIAAKKCTPPGEKQFRGYTLAQFEEADRVYCASGAAAPRLRLITPPSD"
     gene            complement(3822..4037)
                     /locus_tag="VM910_10630"
     CDS             complement(3822..4037)
                     /locus_tag="VM910_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63023.1"
                     /translation="MTDPQDIDVTLVALGFRQGEAGTLIVPADSVVRLIPTGKFYELN
                     ISLGDGNAVTAVLSRTALKFSRVGVTR"
     gene            4343..4708
                     /locus_tag="VM910_10635"
     CDS             4343..4708
                     /locus_tag="VM910_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63024.1"
                     /translation="MSKKPNRFVVQVRNGVVLFFNRTTEVEDGWWWGSDADPLQQGPF
                     ASSHEAVANARRDDKRFKLVCWDRAALARDDRELRPTGHVVLIDTGGRLWAKTGEHPA
                     REGWKDQTPATSLGQCGAF"
     gene            complement(4722..5048)
                     /locus_tag="VM910_10640"
     CDS             complement(4722..5048)
                     /locus_tag="VM910_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVI63025.1"
                     /translation="MLVALRTAACRQQLGLCYWCGDQMFTDVEPIHPKFCTADHLIPR
                     YAGGKTVAGNIVAACRACNNARNAPETNRRRAAEPGLVMSIGDDAPCSPFEALKSRAG
                     SVPGPA"
     gene            complement(5060..5308)
                     /locus_tag="VM910_10645"
     CDS             complement(5060..5308)
                     /locus_tag="VM910_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63026.1"
                     /translation="MASSATFTFNWFTISGLVHDAAPDLGETELREAYESLIARGRLS
                     PDPSPSLTKLETLIWDVIIRLAKEHACRDADTATACTD"
     gene            complement(5331..5594)
                     /locus_tag="VM910_10650"
     CDS             complement(5331..5594)
                     /locus_tag="VM910_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63027.1"
                     /translation="MRDRFAELVNAVTAHYRRRLTAEVADPAARLAGFPCETCEAPQA
                     ADVRHLAAQVADVSELALSPGGLPLACCKVKLVGGMGGRPTSW"
     gene            complement(5558..5953)
                     /locus_tag="VM910_10655"
     CDS             complement(5558..5953)
                     /locus_tag="VM910_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63028.1"
                     /translation="MYGDKIAAHTEKLVRDIQNELTVAIERRFGELMGRLDAILPDNT
                     RRAEKTFRFSSERDDGEIVDLPNPLGVVRKTTINRCRPSISFKKRVVAALTGAPAMNR
                     RTCSSVQLRWTFTIANLKCAIASPSWSMR"
     gene            complement(6156..6458)
                     /locus_tag="VM910_10660"
     CDS             complement(6156..6458)
                     /locus_tag="VM910_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HVI63029.1"
                     /translation="MRKTTLVAASILVLAAVLPKVTVAQSLMINGQVTKIDESAGKIT
                     LRHGAIKKLDMNEGMTMVFRVQDPAMLKQVKVGDKVRFDADRVNGQITVTKIERVK"
     gene            complement(6491..6910)
                     /locus_tag="VM910_10665"
     CDS             complement(6491..6910)
                     /locus_tag="VM910_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HVI63030.1"
                     /translation="MPVPASAHEDHGRFSAGEPGDPNKPARIVKVRMFEGGGKMGFEP
                     ARIEVRRGEQVRFLLHNNGEEDHEFILATVAENRKHAEVMKKHPDMEHDDPNGKRILP
                     YGSGELVWKFTKRGEFEFACLIPGHYEKGMFGKIIVK"
     gene            complement(7007..8383)
                     /locus_tag="VM910_10670"
     CDS             complement(7007..8383)
                     /locus_tag="VM910_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HVI63031.1"
                     /translation="MISRRSFIGTAAALAGASAVSGRAQAASIPEAPSTKTTTMQPPI
                     FPVSGPDYQPVVTLNGWTLPWRMNGDWKEFHLVAEPVVREFAPGMKVNLWGYNGQAPG
                     PTIEAVEGDKVRIFVTNKLPEHTTVHWHGMLLPNGMDGVGGVTQPHIEPGQTFVYEFE
                     MKKSGTFMYHPHSDEMVQMAMGMMGMIVVHPRDPKFRRVDRDFVFIMSAYLIDPGTYL
                     PKVAEMTDFNMWTWNSRVFPGIDPMPVRLGDRVRVRMGNLTMTNHPIHLHGHHFAVSC
                     TDGGWVPESAQWPESTIDVPVGMIRAFDMVADNPGDWPFHCHKTHHAMNAMGHDIRNF
                     IGVKKRDLARQVRKLVPDYMPMGSEGMAMMGEMEMPMPDNTLPMMTGFGQFGPIEMGG
                     MFTVVKIREGLAANDYKDPGDYKHPQGTVAYAVASSSAPTAPRNGPASQPKAGPKTVR
                     PGNNHKRH"
     gene            complement(8397..>9134)
                     /locus_tag="VM910_10675"
     CDS             complement(8397..>9134)
                     /locus_tag="VM910_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVI63032.1"
                     /translation="TARQREASEREALIRALGLWGASLDFKLANALAAVPRRVQTLAR
                     IEVDAVARRVDLQIARIELDVLAKSYGLTQATRFINILDAGYADKLERRVDERFLMRG
                     FDVRLQIPIFDFGEVRVRQAEATYMQSVNRLLELAVNARSEARDAYRSYRSAYDIAGH
                     YQREVLPLRKIISDETLLRYNAMLIDVFSLLAEGRQRIAATIAAIEAKRDFWLATVNL
                     KAAVAGGGSGNRADETKPMSSGGMGRN"
BASE COUNT         1651 a         2850 c         2777 g         1856 t
ORIGIN      
        1 gagctcgaga tgacgcgcag gatgtcgccg gtcgcggtct ggtattccag cgactcggtc
       61 agctcgcgcg tgcgctgctg ctcggcctgg aacagccgca cattctcgat cgcgatcaca
      121 gcttgatcgg cgaaggtagt gaccagctcg atctgcttgt cggtgaacgg acggaccgtg
      181 gaccgagtga ccacgaggac accgatcgga acgccttccc gcagcagagg cacaccgagc
      241 acggtgcgat tcgctccgat cttcaggact ggcgaatcgt attccggatc ggccagtaca
      301 tccggaatgt gaaccgtctt gccttcgagg agcgttcgac cggtgattgt gtcgcggccc
      361 ggtcgaatcg cgagccgctt gagcgtctgt tcatgctcag ccgagagcgc aaacgtcgcg
      421 gcaaccctgt agacgtctcc gctcggccgc cagatgttgg cacgctcggc ttcacacagc
      481 cgcgccgccg attcgaccag cgtgtcgagc accgtctgca gatcgaaggt cgagcggctg
      541 atgaccttga gcacgtcggc ggtggcggtc tgctgctgga atgattcgcg cagctcgttg
      601 agcagccgag tgttctcgat ggcgatgacg gcgtgattgg cgaaattagc gagcattgcg
      661 acctgtttgt cggtgaaggg ccgaatttcc tgccgaaaga tggcgataac tccaatcagc
      721 tcatcgtcct tgagcatcgg cacactgacg gcggtccgaa caccgccaag ctctacggca
      781 tcgacggtga ctggatggcg ttcggcataa gcttgtgacg ctgccatgtc ggcagtctgg
      841 accgtgtgct tggttctaat cacctcgtta agaggaccgc ccggaggcgg atggaacggg
      901 ccacgccgcc gcgcctcggc aaacgctgcc ggcacattgt ggctggcgac gaggcgtaat
      961 gccccgcgtt catggaggta gagcgtgccg aacttggcct cacagatgcg cactgcatta
     1021 gccagcatgg cctgaaacac cggctcaagc tcgcccggcg aagtgctgat gacttgcaac
     1081 acctcggacg tagccgcctg ctgctccaac gcctcggaaa ggtgccctcg cgcctcggcg
     1141 agttcacggg tgcgcacttc gagttgcttc ttcaactcga ccagtgaata gggcccattg
     1201 ctaacgcgcg ctgccgcctt cgttccagtc gaacgaagct tgcggccgcg tgttgggcta
     1261 tttgcgcctt tgagagacaa ggtcacctcg tcggcacagc cactggtacc ccttccggtc
     1321 gggcccggcc aaggatcgag cctatcaatc ctaacagaac cgcacgctga actcgcgcct
     1381 tttcacactt gatcgcctcg tcgccactag tcgcagaacg ggagattatg gaatatcggt
     1441 ccgattgccg gatcactccg gcttgatgcc ggcgaacttt accaccttcg cccatttctc
     1501 ggtttcttcg gcgattaact tgccgaagtc gctgggcggg cctggaagca cagtgccgcc
     1561 aaggtcagca aggcgcgcct tcatcttggg atcggcaagg ccagcgttaa tctccttgtt
     1621 gagcttgtcg acgatctcgg ccggcgtggc cctcggtgcg ccgacgccgg accaactgct
     1681 tgcctcgtag ccaggcacaa attccccgac agtcggaaca tcgggcagcg cctccgaacg
     1741 agtcgcggtg gccaccgcga gggcgcgcag cttgccggcc ctgatgtatg cgatcgacga
     1801 gggcatggtg gcgaacatga cctgcacctg cccgctaagg aggtcggtga gagcgggccc
     1861 accgccgcga tacggcttgt gagccacccc cggtcggcgc agaaattgcc gtcagatcaa
     1921 gccattacga aaacggggct gcgcgggtgt cgcgggagtg tcccggggaa ctcgccgcgg
     1981 cagagcggtt aatcctgcgc tcagtctagc ccaagtttac ggtgtatctc agccagctcg
     2041 cgacggctct tttcaaacct ctcatcgccg acggcctgcc gaaaccgctc gtcgcggcag
     2101 cgagtgctaa gctctgtctc ttcacgttct cgccgctcaa tttcacgctt caacagctct
     2161 aggaggtgtt cgtaatcgcc cgactgcaaa tacggttgca agtctctctt tagcgcttgc
     2221 aaccctgcaa acttcagccg cgcgcgcttc agccgccgtc gcacccgtac cagctcattg
     2281 gttagctcgt gcagtagatc ggtggacgag accttcgcca accccggctc attgataacc
     2341 ttcatgatcg actcctttcg gcgatctgtg gcgcctgcga taccccctcc ccgataaggc
     2401 cgtcccaagt gtcccaaccg tccctcaata gcgcgccgat cacagccgcg gcgggacgcg
     2461 tgggacggga aggacgccct aatcggaggg gggcgtgatc agacgcagac gcggcgcggc
     2521 ggcgcctgac gcgcagtaca cacggtcggc ctcctcgaat tgcgcgagcg tgtagccgcg
     2581 gaattgcttc tcccccggcg gcgtgcactt cttcgctgcg atgccgacca ttcccagcag
     2641 cttgacctgc tcgttcatcg tgagcggatg tgctttgtcg aggccgctga ggtcgcggta
     2701 tcgcgtccag tccgcatcgg gcaaatcgag gcgcttgagt tctttattga actgagtgct
     2761 cctgatctgc ttgatcccga gtgcatcgaa gatcgcgagg ccatggcgga tcatgcggat
     2821 tttcggctgt tctgtcctcg ccttctctat caggaacagg atcgcctcgc gggcgcgctg
     2881 tccccactcc ggcccgcagg cgtcggcgac cgcgagcaag ctacacgcgt tatcggcatc
     2941 gcgggcgatc acccccggcg gcatcggcgg atcaagattg agcttcatat tcgcggtccg
     3001 gtgccgaagg aacgaataca cggcgtcgag gtccgcggta tcggtagcct tgaacttccg
     3061 ctctggcttt gtctctgcgg tgtacttgtg catctcaaga atgaacgtcc gcgtcatctg
     3121 cgcatctgcc aggaacgagc cgaggcctgc agccatcatc ggcgcgtgaa agtcgaccac
     3181 ctttcgcttc cctttcacca tcagcgcgcg cttcgccagc gcgcggtcgt gccctaaatt
     3241 ccagagcagc agaatgtatt gtcgcgcttc cgcgtcgaga agatcgagct cgtcgagtcc
     3301 aatcgtgcac ggacccgcgt cgatgaaatc gatgaccgcc gcccccgtgc tgagcgcctc
     3361 cgggttgggc ctcagcatca ggcatttggc aactttcagc gcctcgctct tgccgctgcc
     3421 tggtaactcg ctaaccaatg ccagacgcgc ggcaatgtcg aattggcgat agacgtgcgt
     3481 ggcgacgatg tacaacgagt agatgacttg aacgtggggc gtcatcgtca ggtacttgcc
     3541 gacaattccg tgcactaatc cgacaggggt gaacttcgga tcctcgaacg ggtgcggctg
     3601 tgcatcacgc ggatcggacg gcggcggtga gggcgcttgt gccgcatcat cggccgcagc
     3661 ctgaacaagg atttcgggaa tgtcgttaat cgtcttgcga tagtgcttga gccaattgtc
     3721 gatctgacgc ttggcattag cacgatcacc ggccgatgtg cttttgtgta tccacaccgc
     3781 atagattttc gcaaaccgct gcgcggggga gcttttcgcc atcagcgcgt cacccctaca
     3841 cgactgaatt tgagcgcggt cctagacaac accgcggtca ccgcgttgcc gtcgcccagg
     3901 ctgatgttga gttcgtagaa cttgccggtc gggatgagcc gcacgacgct gtctgcaggc
     3961 acaatgagcg tgccggcctc gccctggcga aagcctagcg cgacaagcgt gacatcaata
     4021 tcctgagggt cagtcatcct atcgctctga tccagcgata tcggtggcga gacgcctcga
     4081 tggccgtcgc aagcggtcat cggggcgcat tcaagctgcc acggatgagc cgccagcgac
     4141 aaattgcacc gcgcctcgcc gttagctggc gttggccacc attattgtgc gcccgcggta
     4201 agcacttcag acggctgcac taaaaatttt ttcgttaaca atcatacttt cacgctcggc
     4261 gccggtgcgt gatgaccacc gcggtcgata tcatgctggc aatcgtcgtc ctcggcaccg
     4321 ccgcgctcat tgggagtaag ccatgagcaa gaagccgaac cgatttgtcg tccaagtacg
     4381 aaacggcgta gtcctctttt tcaatcgtac gactgaagtg gaggacggtt ggtggtgggg
     4441 ctccgacgca gacccgttgc aacagggacc gtttgcgagc tcgcacgagg cagtggcaaa
     4501 tgcccgtcgc gacgacaaac gcttcaagct ggtgtgctgg gatagagcag cgctcgctcg
     4561 tgacgatcgt gagctccgcc ccaccggtca tgtcgtgcta atcgataccg gcggaaggct
     4621 ttgggcgaaa accggagagc atcccgcgcg cgagggctgg aaggatcaga caccagcgac
     4681 gtctctgggg caatgcgggg ccttctaatg gcggtcgagc atcaggccgg ccctggcacc
     4741 gaacctgccc gggacttgag cgcctcgaac ggcgagcacg gcgcatcgtc gccgatcgac
     4801 atcacgagac caggctcggc cgcgcgccgg cggttggtct cgggcgcatt gcgagcgtta
     4861 ttgcatgccc ggcaggcggc gacaatgttg ccggccacgg tcttaccgcc cgcgtagcgc
     4921 gggatcagat gatcggccgt acagaacttg gggtggatcg gctcgacgtc cgtgaacatc
     4981 tggtcgccgc accagtaaca cagacccagt tgctgtcggc acgccgccgt gcgcagcgcg
     5041 acgagcaaat tgtgtccgct taatcggtac aggcggtggc ggtgtccgcg tcgcggcatg
     5101 catgctcttt cgctaggcgg atgatgacgt cccagatcag cgtttccaac ttggtgagcg
     5161 acggtgacgg atcgggtgaa agacgaccac gcgcgatcag gctttcgtac gcctctctaa
     5221 gctccgtctc gccaagatca ggagcggcat cgtgaaccaa gcccgagatt gtgaaccagt
     5281 tgaacgtgaa cgttgccgac gaagccatcg cggattctcc tgcgtcggca tcaccacgag
     5341 gtcggcctgc cacccatccc gcccaccagt tttactttgc agcaagcgag cggtagccca
     5401 cccggcgaca gcgcgagctc gctcacatct gcgacctgcg cggcgagatg tcggacatcg
     5461 gccgcttgcg gtgcctcgca cgtctcgcaa ggaaacccgg caagacgcgc agccggatcg
     5521 gcaacctcag ccgtgagcct gcgccggtaa tgagcagtca ccgcattgac cagctcggcg
     5581 aagcgatcgc gcacttcaga ttcgctatcg taaacgtcca gcgcaactgc acagaggaac
     5641 acgtccgccg attcatcgct ggcgcgccag taagcgcagc cacgacccgc ttcttgaacg
     5701 aaatagacgg ccgacatcga tttatcgttg ttttgcgcac aactccgagc gggtttggca
     5761 gatcaacgat ctcgccgtca tcacgctcgc tgctgaaccg gaaagttttc tccgcccggc
     5821 gagtgttgtc gggcaggatg gcgtcaaggc gacccatcag ttcgccaaac cggcgctcaa
     5881 tcgcaacagt cagttcattc tggatatccc ggacgagctt ctcggtgtga gcggcgatct
     5941 tgtcgccgta cataccgagc gcctcttccg tcgccagccg gatctttgcg acagcatcga
     6001 agccgtccgc gaaaatgacc ccttccgttc cgagctcggc accgtgctaa tgcagtggaa
     6061 ggaaaagctc ggcagaaagg catctcaacg ccggggatgt actagccctt aaacgcattt
     6121 tgttgcgctg cgggcatctt gatctcgcaa aataatcact tgacccgttc gatcttcgtg
     6181 acggtgatct gcccgttgac gcgatcggcg tcgaaccgga ccttgtcgcc gactttcacc
     6241 tgcttgagca tggctggatc ctgcacgcga aagaccatcg tcattccctc gttcatatcg
     6301 agctttttga ttgctccgtg cctaagcgtg attttgcccg cggactcgtc gatcttggtc
     6361 acctgaccgt tgatcattag agactgcgcc acggtcacct tcggcagcac cgcggctaga
     6421 acgagaatac ttgcggcaac gagagttgtc ttgcgcatgg attgtctcct tttggttttg
     6481 ctcgtgcacc tcacttcacg atgatcttgc cgaacatgcc cttctcgtag tggccgggga
     6541 tgagacaggc gaattcgaat tcaccgcgct tggtgaactt ccaaacgagc tcgccgctgc
     6601 cgtaaggcag aatgcgcttc ccgttcgggt cgtcatgctc catgtcggga tgttttttca
     6661 tgacctccgc gtgtttgcga ttttcggcaa ccgtcgcgag gatgaattcg tgatcctcct
     6721 caccattgtt gtgcagaagg aaacggactt gctcccccct gcgcacttcg attcgcgcgg
     6781 gctcgaagcc catttttccg ccgccctcga acatcctgac cttgacgatg cgcgccggct
     6841 tgttcgggtc gccgggctcg ccggcggaga agcgtccgtg atcctcgtgg gcggacgccg
     6901 gaacgggcat tgcgacaccc gtcgccagga ttgcggcgct cgccgtcagc aaaatgccga
     6961 gggcggtaaa ggagcgcgta tatttcagca tgctgacttg gctccttcag tgtcgcttgt
     7021 ggttgtttcc aggcctgacc gtcttggggc cggctttcgg ctggctggca gggccgtttc
     7081 gcggggccgt gggcgcggat gagctcgcga ccgcgtatgc gaccgtgcct tgcggatgct
     7141 tgtagtcgcc cgggtccttg taatcgttcg ctgccaagcc ttcgcgtatc ttcacgacgg
     7201 tgaacatgcc acccatctcg atcggcccga attggccgaa gcccgtcatc atcggcagcg
     7261 tgttgtcggg catcggcatt tccatctcgc ccatcatcgc cattccctcc gagcccatcg
     7321 gcatgtagtc ggggacgagt ttgcggacct gccgggcgag atcgcgcttc tttacgccaa
     7381 tgaagttcct gatatcgtga cccatggcgt tcatggcgtg gtgcgtcttg tgacaatgga
     7441 acggccagtc gcccgggttg tcggcaacca tgtcgaaggc ccggatcatg ccgaccggga
     7501 cgtcgatggt gctttcgggc cattgcgcgc tttcgggcac ccaaccgccg tccgtgcagc
     7561 tcaccgcgaa atggtggccg tgcagatgga tcgggtggtt ggtcatggtg agattgccca
     7621 tgcgtacgcg cacgcggtcg ccaagccgca ccggcattgg atcgatgccg ggaaacacgc
     7681 ggctgttcca ggtccacatg ttgaagtcgg tcatctccgc gaccttcggc agataggtcc
     7741 cgggatcgat caggtaagcg ctcatgatga atacgaagtc gcgatcgacg cggcgaaact
     7801 tcgggtcgcg cgggtgcacg acgatcatgc ccatcatgcc catcgccatc tgcaccatct
     7861 cgtccgagtg ggggtggtac atgaacgtgc cgctcttctt catttcgaac tcgtagacga
     7921 acgtctgtcc gggctcgatg tgcggctgcg tcacgccgcc aaccccgtcc atgccgttcg
     7981 gcagcagcat gccgtgccag tgcacggtcg tatgctccgg cagcttgttg gtgacgaaaa
     8041 tgcggacctt gtcgccttcg acggcttcga tcgtcggtcc gggcgcctga ccgttgtaac
     8101 cccacaaatt caccttcatg ccaggggcga actcgcgcac caccggctcg gcaacgaggt
     8161 ggaactcctt ccagtcgccg ttcatgcgcc agggcaaggt ccagccattg agcgtgacga
     8221 ccggctgata gtcggggccg ctcaccggga agatcggcgg ctgcatggtg gtggtcttgg
     8281 tgcttggcgc ctcgggaatg ctcgccgcct gcgcgcgtcc gctgaccgcg ctcgcacccg
     8341 cgagcgccgc cgcggtgccg atgaagcttc ttcgcgaaat catgccattc tcctcgctag
     8401 tttctgccca tgccgccgga cgacatgggc tttgtttcat ccgccctgtt gccggagcca
     8461 ccgccggcaa cggccgcctt gaggttcacc gtggcgagcc agaagtcgcg cttcgcctcg
     8521 atggccgcga tcgtcgcggc aatgcgctgg cgcccctcgg cgagcagcga aaacacgtcg
     8581 atcagcatcg cgttgtagcg aagcagcgtc tcgtccgaga tgatcttgcg cagcggcaag
     8641 acctcgcgct ggtagtggcc ggcgatatcg taggcggaac gatagctgcg gtaggcgtcg
     8701 cgcgcctccg agcgcgcatt gaccgccagc tcaagcagtc ggttcacgct ctgcatgtat
     8761 gtcgcttcgg cctgacggac gcggacctcg ccgaaatcga agatggggat ttgtaatcgg
     8821 acatcgaagc cgcgcatcaa aaagcgttcg tcgacacgcc tctccagctt gtcggcgtag
     8881 ccggcgtcga ggatgttgat gaagcgggtg gcttgggtga gtccgtagga tttcgcgagc
     8941 acgtcgagct cgatgcgggc gatctgcaga tccacgcgtc gggcgaccgc gtccacctcg
     9001 atcctggcca aggtctggac acgacgtggc acggccgcaa gcgcattcgc aagcttgaag
     9061 tcgagacttg cgccccagag gcccagcgcg cgaatgagcg cctcgcgctc gcttgcctcg
     9121 cgctggcgcg cggt
//
LOCUS       DATLJT010000462         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3243788,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000462 DATLJT010000000
VERSION     DATLJT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243788"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VM910_10680"
     CDS             complement(<1..618)
                     /locus_tag="VM910_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HVI63033.1"
                     /translation="MITASVAGAATLSSAQTVPVPKPAPKGRDNTQMSASEAQKRPLT
                     TGTTSATPPEPVIPDPHRNVPTNIFASFDASQKAQAAKVSSYLSSLQNLVGNFVQVGP
                     DGTKTKGDFYIQKPGKVRFAYDPPTPIDIIADGSSLAVRDRNLATQDIYPLSQTPLRF
                     LLSDRIDLLKDTNVVNVSADDLFVTITIEEKQALIGTSRLMLMVGA"
     gene            complement(807..>2671)
                     /locus_tag="VM910_10685"
     CDS             complement(807..>2671)
                     /locus_tag="VM910_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HVI63034.1"
                     /translation="TPIEDDDAPFVEEEEDDGSISLGWAFHALMSAKARLVWLLSAAY
                     RSLVSSGPPPRAITLDRQEPNLGKAAPSLAPQADDSDEDEEDEEDEEDEDEPVARARR
                     KKAAPRAPSRKSSDKFDLPSVSMLAAPKASDRQPLSKNELENNSRALEGVLQDFGVRG
                     EIVKAHPGPVVTLYELEPAPGIKSSRVIGLADDIARSMSALSARVAVVPGRNAIGIEL
                     PNAHREKVYLREILTAREATESVAKLPLCLGKNIGGDSIIVDLARMPHLLIAGTTGSG
                     KSVAINTMILSLVYRLRPDQCRLIMVDPKMLELSVYDGIPHLLTPVVTDPKKAVVALK
                     WAVREMEERYKNMAKLGVRNIDGYNARLAEGRSRGEELTRTVHTGFDKETGKAIYEEE
                     KLDLEPLPYIVIIVDEMADLMMVAGKDIEGAVQRLAQMARAAGLHVILATQRPSVDVI
                     TGTIKANFPTRISFQVTSKIDSRTILGEMGAEQLLGQGDMLYMAGGGRISRVHGPFVS
                     DEEVEKVVRHLKTQGSPEYLEAVTAEEPGEEDGAVFDSTGMGADGGGDLFAQAVAIVK
                     RDRKASTSYIQRRLQIGYNRAASLMERMELEGIVGQANHAGKREILVPEEEGGF"
BASE COUNT          438 a          841 c          843 g          549 t
ORIGIN      
        1 ggcgccgacc atcagcatca gacggctggt gccgatcagc gcctgtttct cctcgatggt
       61 gatggtgacg aagagatcgt cggccgacac gttcaccacg ttggtgtcct ttaacaggtc
      121 gatgcgatcg gacagcagga atcgcagcgg tgtctgcgac aacgggtaga tgtcctgggt
      181 cgccagattg cggtcgcgca ccgccagcga tgaaccgtcg gcgatgatgt cgatcggcgt
      241 cggcggatca taggcgaagc ggaccttgcc cggtttttgg atgtagaaat cgcccttggt
      301 cttggtgccg tcgggcccga cctgcacgaa attgccgacc agattctgca gtgaggagag
      361 ataggagctc accttggcgg cttgcgcttt ctgacttgca tcgaaggatg caaagatgtt
      421 ggtcggcaca ttgcgatgcg gatcggggat gacgggttcc ggcggcgttg ccgatgtcgt
      481 ccccgtcgtc agtgggcgct tttgcgcctc cgacgcgctc atctgcgtgt tgtcgcgacc
      541 cttcggcgca ggttttggca cgggaactgt ctgcgccgac gacagcgtcg ctgcgcctgc
      601 gacggaggcc gtgatcatca ccacgaaaac aacgcgcgcg ccggcaaatg cgagcgatcc
      661 ggccggtcgg gtttttcgat ttctaatcaa cgcccaatcc tgaatttgct ttcaaagagt
      721 gtatcgcgcc tcgggagcga ggggatatcg tttttccgtg aaaatgccag cctgcttcac
      781 cccaacacta atccagattg cggctttcaa aagccgcctt cttcctcggg taccagaatc
      841 tcgcgttttc ccgcgtgatt ggcctggccc acaatgccct ccagttccat ccgctccatc
      901 agcgacgccg cgcggttata gccgatttgc agccgccgct ggatatagct cgtcgaggcc
      961 ttgcggtcgc gcttgacgat tgcgaccgcc tgcgcgaaca ggtcgccgcc tccgtccgcg
     1021 cccatgccgg tcgaatcgaa caccgcgcca tcctcctcgc ccggctcttc ggcggtgacg
     1081 gcctcgagat attccggcga gccctgcgtc ttcagatgac gcaccacctt ctcgacctct
     1141 tcgtcggaga cgaaggggcc gtgcacgcgg ctgatgcgtc cgccgcccgc catgtagagc
     1201 atgtcgccct gcccgagcag ttgctcggcg cccatctcgc cgagaatggt gcggctgtcg
     1261 atcttcgacg ttacctggaa tgaaatgcgg gtcgggaagt ttgccttgat ggtgccggtg
     1321 atgacgtcga ccgacggccg ttgcgtcgcg aggatcacgt gcaacccggc ggcgcgcgcc
     1381 atctgcgcga gccgctgcac cgcgccttcg atgtccttgc ccgcgaccat catcaggtcg
     1441 gccatctcgt cgacgatgat gacgatgtag ggcagcggct cgagatcgag cttttcttct
     1501 tcgtagatag ccttgccggt ttccttgtcg aagccggtgt gcacggtgcg cgtcagctcc
     1561 tcgcctctgg atcgtccttc cgcgagacgc gcgttgtagc cgtcgatgtt gcgcacgccg
     1621 agcttggcca tgttcttgta gcgctcttcc atctcgcgca ccgcccattt cagcgcgacc
     1681 acggccttct tcgggtcggt cacgaccggg gtgagaagat gcggaatgcc gtcatagacg
     1741 gagagctcga gcattttcgg atcgaccatg atcagccggc actggtcggg acgaagccga
     1801 tacaccaggc tcaggatcat ggtgttgatc gcgacggatt tgccggagcc ggtggtgccc
     1861 gcgatcagaa gatgcggcat gcgcgcgaga tcgacgatga tggaatcgcc gccgatgttc
     1921 ttgccgaggc agagcggaag tttcgccacc gactcggttg cttctctcgc ggtcaggatc
     1981 tcgcgcaaat agaccttctc gcgatgcgcg ttcggcagct cgatgccgat ggcgttgcgg
     2041 ccgggaacga cggcgacgcg cgccgacagg gcgctcatcg agcgcgcgat gtcgtcggcg
     2101 agcccgatca cgcgcgaaga cttgatgccg ggagcaggct cgagctcgta gagcgtgacc
     2161 acgggtccgg gatgggcctt gacgatctcg ccgcgcacgc cgaaatcctg cagcacgcct
     2221 tccagcgccc gcgaattgtt ttcaagctcg ttcttcgaga gcggctggcg gtcggatgct
     2281 ttcggcgctg ccagcatcga cacgctcggc agatcgaact tgtcggagga tttgcgcgaa
     2341 ggcgcacgag gggccgcctt cttgcgccgc gcgcgagcga ccggttcgtc ctcatcctcc
     2401 tcgtcctctt cgtcttcttc gtcctcgtcg gaatcgtcgg cttgcggcgc gagcgatggc
     2461 gcggccttgc cgagattggg ctcctggcgg tcgagggtga tcgcgcgcgg cggcggaccg
     2521 cttgaaacca gcgaacgata ggccgcgctc aacagccaga cgagccgcgc tttcgcgctc
     2581 atcagtgcat gaaacgccca cccgagcgag atggatccgt cgtcctcctc ctcttcgacg
     2641 aacggcgcat cgtcatcctc gatcggcgtc a
//
LOCUS       DATLJT010000463         3908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1635142,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000463 DATLJT010000000
VERSION     DATLJT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635142"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VM910_10690"
     CDS             <1..876
                     /locus_tag="VM910_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI63035.1"
                     /translation="SSLVMHPIYAYGDENQRKKYLPKLATGEWVGCFGLTEPDAGSDP
                     GGMKTRAEKVSDGYRLTGSKMWISNAPIADVFVVWAKSAEHNNQIRGFILEKGMKGLS
                     APKIGGKLSLRASITGEIVMDGVVVPESALLPNVSGLKGPFGCLNRARYGISWGAMGA
                     AEDCMHRARQYTLDRKQFKRPLAATQLVQKKLADMQTEIALGLQGSLRVGRLMDEGKI
                     TPEMISIVKRNNCGKALDIARACRDMHGGNGIQIEYHVMRHLSNLETVNTYEGTHDVH
                     ALILGRAITGIQAFS"
     gene            1006..1926
                     /locus_tag="VM910_10695"
     CDS             1006..1926
                     /locus_tag="VM910_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63036.1"
                     /translation="MAENDDVPFNRNFPLKPGLVEEVRPGVRRVLCDNPSPFTFTGTV
                     SYIIGNGKVAIVDPGPDSEAHAKALLDAVRGETVTHILVTHTHRDHSPNTARIKQATG
                     ATVYAEGPHRASRPRHESERHNPESGADRGFRPDIEVRSGDVIEGDGFRLEAVATPGH
                     TANHMTFAWPERKMSFVGDHVMGWSTSIVAPPDGSMVDYMASLERLANRDENLYFSGH
                     GPEIPEGPRYVNFLIRHRQAREASILHRLAKGEADIPTMVRAIYIGIDPRLVTAAGYS
                     VLAHLEDLVARGIVATDGDPVIGGTYRLAS"
     gene            complement(1964..2824)
                     /locus_tag="VM910_10700"
     CDS             complement(1964..2824)
                     /locus_tag="VM910_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HVI63037.1"
                     /translation="MARRFSAPYQSEPVSSLASWARNLAVFSVVAVLVSIIIVRFGFL
                     EMKPALATFFGALGCAVLSILVGLLGFVAIWRNGSRGMARILLAFMISAVVLAYPAYL
                     ALQYRRLPAIHDITTDPIDPPRFEALARLRTGDGANTAVYAGLYSAELQQKAYPDIEP
                     LQLEIPVDRAYAVTLQLVNRRKWLVIDERPPAPPRRVGRIEAVARTPIMGFREDVAIR
                     IAPDGALDSRIDIRSSSRYFESDLGSNAARVTKFIEDLSAAAEADAQKPAKKIAAPPP
                     KAPPAKTVKK"
     gene            complement(3035..>3908)
                     /locus_tag="VM910_10705"
     CDS             complement(3035..>3908)
                     /locus_tag="VM910_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI63038.1"
                     /translation="AKLDGASVYELMHTEKVTHTAGVPTVWLMLLQYMAANNLKLPHL
                     RFVVCGGSAMPRSMIKAFVDMGVTARHAWGMTEMSPIGTLAALKPPFSEHAGAEKLDI
                     LQSQGYPPFAVEMKITDDAGKELPWDGKTFGRLKVAGPAVAKAYFRVDADILDDEGFF
                     DTGDVATIDPHGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPK
                     WDERPLLIIQLKPGQKVTREDILKFMDGKIAKWWMPDDVVFVEGIPHTATGKILKTAL
                     RDQFKTYSFPNAAG"
BASE COUNT          741 a         1211 c         1267 g          689 t
ORIGIN      
        1 tcctcgctgg tgatgcatcc catctatgcc tatggcgacg agaaccagcg caagaaatat
       61 ctgcccaagc ttgcgacagg cgagtgggtc ggctgtttcg gcctcaccga gcccgatgcc
      121 ggttccgatc ccggcggcat gaagacccgg gcggaaaagg tttcggacgg ctaccggctg
      181 accggcagca agatgtggat ttcgaacgcg ccgatcgcgg atgttttcgt ggtctgggcg
      241 aagtcggccg agcacaacaa ccagatccgc ggctttattc ttgaaaaggg catgaagggt
      301 ctttccgcgc cgaagatcgg cggcaagctg tcgctgcggg cttcgatcac gggcgagatt
      361 gtcatggacg gcgtggtggt gccggagagc gccttgctgc ccaacgtgtc cgggctgaaa
      421 ggcccgttcg gctgtctcaa ccgtgcccgc tacggcattt cctggggcgc gatgggcgcc
      481 gccgaagact gcatgcatcg cgcgcggcaa tacacgttgg acagaaaaca gttcaagcgg
      541 ccgctggcgg cgacgcaatt ggtgcagaag aagcttgcgg acatgcagac agagatcgcg
      601 ctcggcctgc agggttcgct gcgcgtcggc cgtctcatgg atgaaggcaa gataacgccc
      661 gagatgatct cgatcgtcaa gcgcaacaat tgcggcaagg cgctcgacat tgcgcgtgcc
      721 tgccgcgaca tgcacggcgg caacggcatc cagatcgagt atcacgtgat gcgccacctc
      781 tcgaacctgg aaaccgtcaa cacctatgag ggcacccacg acgtgcacgc gctgatcctc
      841 ggccgcgcca tcacgggcat ccaggcgttt tcgtaagatt cgcacccgtc attccggggc
      901 gcgcgaagtg cgaacccgga attcggggtc acactcttct caatctcatc gagatttccg
      961 gacccggtcc tgcgaccgtt ccggaaatga cgtcaaagag acgacatggc cgaaaacgac
     1021 gacgtcccgt tcaaccgtaa ctttccgctg aaaccaggcc tcgtcgagga agttcgtccc
     1081 ggcgtgcgcc gcgtgctctg cgacaatccg agcccgttca ccttcaccgg tacggtcagc
     1141 tacatcatcg ggaacggcaa ggtcgcaatc gtcgatcccg gtcccgacag cgaggcgcat
     1201 gcaaaagcgc tgctcgacgc cgtgcgcggc gagacggtga cccatatcct ggtcacccac
     1261 acccaccgcg accactcgcc gaacaccgcc cgcatcaagc aggcaaccgg cgccaccgtc
     1321 tatgccgaag ggccgcaccg cgcttcgcgg ccgcgccatg aaagcgagag gcacaatccg
     1381 gaatcgggcg ccgaccgcgg cttcaggccg gatattgaag tcagaagcgg cgacgtcatc
     1441 gaaggcgacg gctttcgcct ggaagcggtt gcaaccccag gccacaccgc caaccacatg
     1501 accttcgcct ggcccgaacg aaagatgtct ttcgtgggag atcatgtgat gggctggtcg
     1561 acctcgatcg tggcgccgcc cgacggctcg atggtcgact acatggcctc gctggagcgg
     1621 ctcgccaacc gcgacgagaa tctctatttt tcgggtcatg gcccggaaat tccggaaggt
     1681 ccgcgctatg tgaatttcct gatccgtcac cgtcaggcac gcgaggcctc gatcctgcat
     1741 cgcctcgcca agggcgaggc ggatatcccg acaatggtgc gcgcgatcta tatcggcatc
     1801 gatccgcgcc tggtgacggc ggccggctat tcggtgctgg cgcatctcga agacctcgtc
     1861 gcgcggggca tcgtcgcaac cgacggcgat ccggtcattg gcgggacgta tcggctcgcg
     1921 tcttgatctc tccccgaagg aacggggaga agcctcacac cgcctatttc ttgaccgtct
     1981 tggcgggcgg cgcctttggc ggaggggctg cgatcttctt tgcgggcttc tgcgcgtcgg
     2041 cctcggccgc agccgagagg tcctcgatga atttcgtgac gcgtgcggcg ttggagccga
     2101 gatcgctctc gaaatagcgc gaggaagaac ggatatcgat ccgcgaatcc agggcgccat
     2161 cgggcgcgat acggatcgca acgtcctcgc ggaaacccat gatcggcgtg cgtgccaccg
     2221 cctcgatccg cccgacgcgg cgcggcggag ccggcgggcg ctcgtcgatc acgagccatt
     2281 tgcgcctgtt gacgagctgc agcgtgaccg cataggcgcg gtcgacggga atttcgagct
     2341 gcagcggctc gatgtcggga taggcctttt gctgcagctc agcggagtag aggccggcat
     2401 agacggcggt gttggcgccg tcgccggtgc gcagacgcgc cagcgcctcg aagcgcgggg
     2461 ggtcgatcgg atcggtggtg atgtcgtgga tcgccggcag cctgcggtat tgcagggcga
     2521 gataggccgg gtaggccagc acgaccgcgc tgatcatgaa ggcgagcagg atccgcgcca
     2581 tgccgcgcga gccgttccgc cagatcgcga cgaagcccaa aagcccgacc agaatggaaa
     2641 gcaccgcgca gccgagcgcg ccgaaaaacg tcgcaagcgc aggcttcatc tccagaaagc
     2701 cgaagcgcac gatgatgatc gacaccagca cggcgacgac cgagaacacc gcgagattgc
     2761 gcgcccagct cgcaagggag gacacgggct ccgactgata gggagcggaa aacctgcggg
     2821 ccatttcaaa aaaatctgct tgagttgaac gggccgggtc ggttgcccga gggcctttgc
     2881 ggcactgttg agaccatgcc gctgaggcaa attcaaggga gtttcagcct ggacaacatc
     2941 cagggatcgt catggtccgc attgcgtgtc cgcgggacat cgggcgcgaa gatggcgttt
     3001 cgcgcctttt gcccggggta tgacagcgga aagatcagcc ggccgcgttc ggaaaactgt
     3061 aggtcttgaa ctgatcgcgc agcgcggtct tcaggatctt tccggtcgcc gtgtgcggaa
     3121 tgccctcgac gaatacgacg tcgtcgggca tccaccattt ggcgatcttg ccgtccatga
     3181 acttgaggat gtcctcgcgc gttaccttct ggcccggctt gagctggatg atcagaagcg
     3241 gccgctcgtc ccatttgggg tgatacacgc cgatcactgc ggcctccgcc accgcgggat
     3301 ggccgaccgc gaggttttcc aggtcgatgg aagagatcca ttcgccgccg gacttgatga
     3361 cgtccttgga gcggtcggtg attcgcatgt agccgtgcgg atcgatggtc gcgacgtcgc
     3421 cggtatcgaa aaagccttcg tcgtcgagga tgtcggcgtc gacccggaaa taggccttgg
     3481 cgaccgcggg tcctgcgacc ttgagccgcc cgaaggtctt gccgtcccac ggcaactcct
     3541 tgccggcatc gtcggtgatc ttcatctcca cggcaaaggg cggatagccc tgcgactgca
     3601 ggatatcgag cttctcggcg ccggcgtgct cgctgaacgg cggcttcagc gcggcgagcg
     3661 tgccgatcgg gctcatctcc gtcatgcccc aggcgtgacg cgcggtgacg cccatgtcga
     3721 cgaaggcctt gatcatcgag cgcggcatcg ccgagccgcc gcacactacg aagcgcaaat
     3781 gcggcagttt cagattgttg gcggccatgt attgcagcag catcagccag accgtcggca
     3841 cgccggcggt atgggtgact ttttcggtgt gcatcagctc gtagacggaa gcgccgtcga
     3901 gcttggcg
//
LOCUS       DATLJT010000464         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1405417,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000464 DATLJT010000000
VERSION     DATLJT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405417"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VM910_10710"
     CDS             <1..804
                     /locus_tag="VM910_10710"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HVI63039.1"
                     /translation="RIPTVVGRIAWKGPQNADDFRFLKSLTTRTPKITLPGPCYIHYR
                     AGRAHIGRDVYPDLDAFWSDLVEAYVQEMRSLADAGCRYLQIDETSLVKLGDPRVRQL
                     LTERGDDWRDLLRTYVGVVNALVAGAPQAMTIGIHVCRSQDPSWQADVGYDPIAEQLF
                     NAMKVDNYFLEYDNARAGTFAPLRSVPEGKFVVLGLVASKVSELESADVLKRRIEEAS
                     RYVKLEQLGLSPQCGFATSAAEHVTVTEEVQRAKLARVVEVAQQVWGSA"
     gene            complement(861..1535)
                     /locus_tag="VM910_10715"
     CDS             complement(861..1535)
                     /locus_tag="VM910_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HVI63040.1"
                     /translation="MSRSRLFSRQAWDKNASIYEVIRTMPFNAQLASGTLSEARFKHY
                     ITQDAHYLIGFGRALTLAAAKAPNPDRIVQFAKAAEGALVVERALHGSFFKRYGITPR
                     EFAQTPLSLACHHYVSYLLATAYGEPYEVLLGALLPCFWIYAEVGRDIHARARAANPY
                     QAWIDTYAGEEFHAAVRAVIAATDEAAADVSPSLRVRMHAAFTRATQLEWLFWDSAFR
                     LEAWPV"
     gene            1760..>2262
                     /locus_tag="VM910_10720"
     CDS             1760..>2262
                     /locus_tag="VM910_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63041.1"
                     /translation="MAKRRTRPARHTIFTPGELAAHHRDMPKTRHGHPFAPDRVPPGF
                     HTIVDPDKHPPQPWRDLPPAPGLPPYRMSLDTMLDPATIRSIENSGKLVFHSVGDTGG
                     VNTPTYIEGVSRFMELDMAFSKRPDRPSFFYHLGDVVYYDGESANYWPEFYEPYLNYH
                     APIVAIPG"
BASE COUNT          461 a          703 c          674 g          424 t
ORIGIN      
        1 cgcattccga cggttgtcgg ccgcatcgcc tggaagggac cacagaacgc cgacgacttt
       61 cgctttctca agtcgctgac cacgcggacg cccaagatca cgctcccggg cccgtgctac
      121 attcattatc gcgcggggcg cgcccatatc ggccgcgatg tctatcccga cctcgacgcc
      181 ttctggtccg acctggtcga agcctatgtg caagaaatgc gctcgctcgc ggatgccggt
      241 tgtcgctatc tgcagatcga cgaaacgtcg ctagttaagc tcggcgatcc gcgcgtgcgt
      301 caattactca ccgagcgcgg cgatgactgg cgcgatcttc tgcgcaccta tgtcggtgtc
      361 gtcaatgccc tcgtcgcggg cgcgccgcaa gcaatgacca tcgggattca cgtttgccgc
      421 agccaggatc cgagctggca agcggatgtc ggctacgatc ccatcgcgga gcagctgttc
      481 aacgcgatga aggtcgacaa ctattttctc gaatacgaca atgcgcgcgc cgggacgttc
      541 gcaccgctgc gatctgtgcc ggagggaaaa ttcgtggtgc tcggcctcgt cgcctccaag
      601 gtttccgaac tggaaagcgc cgatgtcctc aagcggcgga tcgaggaggc aagccgctat
      661 gtcaagctcg aacaactggg tctgagcccg caatgcgggt tcgcgaccag cgccgcagag
      721 cacgtcacgg tgaccgaaga ggtccaacgg gcaaagcttg ctcgcgtggt ggaggtagcg
      781 cagcaggttt ggggcagcgc gtgatccaca acgccacgcc actgatactc tactgaacga
      841 gactaaacgg gccaggtcta ttaaacgggc caagcctcca accggaaagc cgaatcccag
      901 aacagccact cgagctgggt cgcgcgcgtg aaggcggcat gcatccgcac gcgaagggac
      961 ggcgacacgt ccgccgccgc ttcgtccgtg gcggcgatga cggcgcgcac cgcggcgtgg
     1021 aactcctcgc ccgcgtaggt gtcgatccag gcctgatacg ggttggccgc tcgggcgcgg
     1081 gcgtggatgt cgcgcccgac ctccgcgtag atccagaagc agggtagtag cgccccgagc
     1141 agcacctcgt agggctcccc ataggcggtg gcgagcagat acgaaacgta atggtgacat
     1201 gccagcgaga gcggggtctg cgcgaactcc cgcggcgtga tgccgtacct cttgaaaaac
     1261 gagccgtgca gcgcccgctc gacgaccagc gccccttccg ccgctttggc gaactgcacg
     1321 atccgatccg gattcggcgc cttcgcggcg gcgagtgtca aagcacgccc aaagccgatg
     1381 aggtagtgcg cgtcctgcgt gatgtaatgt ttgaagcgcg cttcgctcaa cgtgccggac
     1441 gcgagctgcg cgttgaaggg catcgtgcgg atgacctcgt agatggaggc gttcttgtcc
     1501 caggcttggc gcgagaagag cctcgatctg ctcatggtcg gtgtccgtcg ctttcactta
     1561 tagccatcag caatcgcaca agtgctaccg cacaaacaac gtaagagtgt aaaacatctt
     1621 gcaacacgct tagattgcat ataacttcag cccccgatcg agaggtgagt ggcgatcatt
     1681 cccgcttaga acctacggcc cgacacaccg atgtttcgcc gttgatcagc cgtcgcatcg
     1741 cgcagaagga tccaagacta tggccaagag gagaacccgg cccgcaaggc atacgatatt
     1801 cacccccggt gagctggctg ctcatcatcg cgacatgccg aaaacacgtc atgggcaccc
     1861 cttcgctcca gaccgggtgc cacctggatt ccatacgatc gttgatcccg acaaacatcc
     1921 tcctcagccc tggcgggatt tgccgccggc gcctggattg cctccatatc gaatgagtct
     1981 tgatacgatg ctcgatccgg ccaccatcag gagcatcgag aacagtggca agctcgtgtt
     2041 tcattccgtc ggcgacaccg gtggtgtgaa tactccgaca tacatcgaag gcgtatcgcg
     2101 ctttatggaa ttggatatgg ccttcagcaa gcggccggat cggccctcgt ttttctatca
     2161 cttgggcgat gtcgtgtact acgacggaga aagcgcaaac tactggccgg agttttacga
     2221 gccttatctc aattatcacg ccccgatcgt agcgatcccc gg
//
LOCUS       DATLJT010000465         9065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_945805,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000465 DATLJT010000000
VERSION     DATLJT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945805"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..440)
                     /locus_tag="VM910_10725"
     CDS             complement(180..440)
                     /locus_tag="VM910_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HVI63042.1"
                     /translation="MLPINGNQLIAIEPVNNLALKFPHDYGIVAVPVNEMDERGNPVL
                     WAHDIPVKAKGDLAAAHGAIYYGALTRDHRERIRLSAGSSQC"
     gene            593..1783
                     /locus_tag="VM910_10730"
     CDS             593..1783
                     /locus_tag="VM910_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63043.1"
                     /translation="MNFYAALQAAGMPEVPREFSVVPQHQVIPSTILAGISDFILCFD
                     RVTAREAWQAAALRDAPATVHLRRPEVCFFSAWDFHLPPEGGWQLIEFNDNGSGFIFA
                     AIINALYYEAAGLGQEQRIMAPARLPAFNQHIGDLVKQEARAFFGEFPAGLFLILDDA
                     ESLQQGKFRKELQLLCDLLHRRGRRAELGCPAEVRWDGQQLLLKGQAVSFIVNRSTDF
                     FWQSEDFCALRKAYHAGRVYVAPNPFTYATRSDKRLLEWLSLPHWDKELGIGLGERRI
                     LSDHVPETHLIRAENVEALAQRKQEFVFKPVHGFAGRGLLDSATVGRARMRRFAKHGE
                     GYVAQKWVPKPSMETDGTPLWTDLRVWAYRGEIYHLSGRASRRLDRLDLTPAGGWLPT
                     YASL"
     gene            complement(1959..2600)
                     /locus_tag="VM910_10735"
     CDS             complement(1959..2600)
                     /locus_tag="VM910_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVI63044.1"
                     /translation="MGAHGRWTDPSGASDEAIRAGAAASMELLVGSNTEETRLFLFSD
                     GSIDRITEESLSRLAAAYGLSAEGLNAYRAAYPGASTGDLFSAIQTDWYWRIPALRMA
                     DAHAAKARAATFMYEFAWRSPQMSGRLGAAHGMEIAFVFDTLGLGTEPMLGRDPPQPL
                     ADAMHRAWVSFAVSGDCGWPQYDPVRRASMRFDLTSKVDNNPLGPALAFWRAR"
     gene            complement(4217..4531)
                     /locus_tag="VM910_10740"
                     /pseudo
     CDS             complement(4217..4531)
                     /locus_tag="VM910_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            4617..4889
                     /locus_tag="VM910_10745"
     CDS             4617..4889
                     /locus_tag="VM910_10745"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI63045.1"
                     /translation="MSGKGPKQTVFAALAEIAKALGHAHRLELLEQLGQGEQSVEDLA
                     TRSDLTFANTSRHLQTLRRARLVDARRHGKRVLYSLASYRRSPSVA"
     gene            complement(5295..5435)
                     /locus_tag="VM910_10750"
     CDS             complement(5295..5435)
                     /locus_tag="VM910_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63046.1"
                     /translation="MKQCREQIGAGIVDLSFQTPGSEHPDALMKALELFDKKVLPHIR
                     DI"
     gene            5931..6197
                     /locus_tag="VM910_10755"
     CDS             5931..6197
                     /locus_tag="VM910_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63047.1"
                     /translation="MKAFLAIATLMPVSAWAAETPTEPYSCWGPQMMWGAGWYGMIFG
                     PLFMMLVLGIVVVIGALIVRSVVGPWQPAPHGPPVDRRPVDILK"
     gene            6266..6619
                     /locus_tag="VM910_10760"
     CDS             6266..6619
                     /locus_tag="VM910_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HVI63048.1"
                     /translation="MKVLFILNGPPYGSETSYNGLRLALALAKRDPSDSITVFLMADA
                     IACGKKGQRTPDGYYNIERMLKRFTLGNHRVLLCGTCMDARGISEAELIEGARRSNMD
                     ELAEATATADRLLVF"
     gene            6735..7001
                     /locus_tag="VM910_10765"
     CDS             6735..7001
                     /locus_tag="VM910_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63049.1"
                     /translation="MIHLGPQKSGLVLGALVGGWHLLWAIIVAAGWGQAFIDFILWMH
                     FLKPVFVIEPFAIGRAVVLILVTGAIGYFIGLIGALLWNRLQTR"
     gene            7008..7625
                     /locus_tag="VM910_10770"
     CDS             7008..7625
                     /locus_tag="VM910_10770"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HVI63050.1"
                     /translation="MPDPARHDARGLSELLAEHARTVSSERVSLAEIADFLGTRSIGA
                     WLLILALPMVLPVPAPGISVLFGMPLMMISAQLALGGRQVWLPAFILRQSLARADYVA
                     LVARMRPAMERFKRLVRPRALWLANDWTKIPIGPICLVLGMIITLPIPLGHVAPGSAI
                     CLLALGLMERDGVVIGIGFVAAVLALVIVTVASAGLIAVRPWLIR"
     gene            complement(7618..>9065)
                     /locus_tag="VM910_10775"
     CDS             complement(7618..>9065)
                     /locus_tag="VM910_10775"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HVI63051.1"
                     /translation="LGTERLPRDHLLHTDLTLKWEYGLRPDLLAVTHVYEGVGSPELV
                     VAAKGAPEAIAELCHLPERDRAELRHAVDRMAQKGIRVLAVARASVPAGFARPETPHE
                     FPFEFLGLVGLADPLRPTVPAAVQECRAAGIRVIMITGDYPQTARAIAAQANLEAGDI
                     VTGAELERMSDADLKERVRSALIFARTMPEQKLRIVNALKANGEVVAMTGDGVNDAPS
                     LKAAHIGIAMGGRGTDVAREAASLILLDDDFTSIVTAVRLGRRIYDNLRKAMAYILAV
                     HVPIAGLALIPLLFDLPLVFWPLHIAFLEMVIDPVCSIVFEAEGEEADTMRRPPRHPS
                     APLFTVGFAVWSLFQGALVLALVAGLFVMALHQKLPETDARALSFAALVATNLGLVLV
                     NRSLGASVFAAFTRPNAALWWVGAATAAILAGVILFPPARELFHFGPLHGDDILVALA
                     SGLIALLLLELAKKLIQPMLPERSGQAPAVT"
BASE COUNT         1696 a         2727 c         2879 g         1763 t
ORIGIN      
        1 acacgcgata gctcggcatc gtgtccctcc ggacgggcga ttccaatctt attgcttgcc
       61 gatcatcaga cccgatgatc tggatcaatt tcggggcgcg cggctggcag ggataaacaa
      121 ggacctgctc agaccagaag ctggaaaggg cgatcccgcc ggaaccgtcg cataccgtct
      181 cagcattggc ttgaccctgc ggaaagtcga attctttctc ggtgatctct cgttaacgca
      241 ccgtaataaa tcgcgccatg ggccgccgcg aggtcgcctt tggccttgac cggaatgtcg
      301 tgcgcccaca gaactggatt gccccgctcg tccatctcat tgaccggaac cgcgacaatg
      361 ccataatcat gcgggaattt gagcgcgaga ttgttaaccg gctcgatcgc gatgagctgg
      421 ttgccgttga tcggcagcac gacatggggc agggtttgat ccgtgtccga aggttccgaa
      481 ttagaggcag agaaattttg ggtttcgtcg gtgaagccca gaggctccca aagccaatct
      541 attccgccag caggtccaga ggataagttt gaagctcaag ccgaggcctc ccgtgaactt
      601 ctacgctgcg ctccaagccg ccgggatgcc ggaggtgccg cgtgaattct ctgtggtgcc
      661 gcaacatcag gtcattccca gcaccatcct cgccgggata tccgatttca ttctctgctt
      721 tgaccgcgtt actgcccgtg aggcttggca ggcggcagcc ttgcgtgatg cgccagcaac
      781 tgtgcacctt agacggccgg aggtttgttt tttcagcgcc tgggattttc atcttccccc
      841 ggagggcggc tggcaactga tcgagttcaa cgacaacgga tccggtttca tatttgccgc
      901 gatcatcaat gctctgtact acgaggcagc cggcctggga caggagcaac ggataatggc
      961 gcctgcccgt ttgcccgcat tcaaccagca cattggagac ttggtcaagc aggaggccag
     1021 ggcattcttt ggagaattcc ctgcaggctt gtttctcatc ctcgatgatg ctgaatcgtt
     1081 gcaacaaggc aaatttcgca aagaactcca gctactctgc gatctgttgc accggcgcgg
     1141 ccggcgggcc gagctcggct gtcccgcaga ggttcgctgg gatggccagc aactgctcct
     1201 caaagggcag gcggtgagct tcatcgtcaa ccgctcgacc gatttcttct ggcagtccga
     1261 ggacttctgc gccctacgga aggcttatca cgcgggtcgg gtctatgtcg cgccaaatcc
     1321 tttcacctat gccactcgca gcgacaaacg gctgctggaa tggctatcgc tgccgcattg
     1381 ggacaaagag ctgggaatag ggctcgggga acgtcggatc ctgagcgacc atgtaccgga
     1441 gacgcatctc atccgcgccg aaaacgtgga agcgctggcg caaagaaagc aggaatttgt
     1501 gttcaagcct gtgcatggct tcgccggccg cgggctactg gatagcgcca cagtggggcg
     1561 ggcgcgcatg cgccgcttcg cgaaacatgg cgaaggctat gtagcgcaga agtgggttcc
     1621 gaagccctcc atggaaaccg atgggacccc cttatggacg gatcttcgcg tctgggccta
     1681 ccgcggggag atttatcacc tttccggtcg agcctcacgg cggctggacc ggctggactt
     1741 gactccggcc ggcggatggc tgccgaccta cgcgtctctt taagccctcc tcaattgcaa
     1801 ccgtagacct ctgaagcacg cggtaagtgg tgagacgatc ggcacgcaca tttcgacaga
     1861 ggtgcagtca tcggctacca cggggacgag catgagtgtc cggtagcagc gggaggggta
     1921 cccggaccat atcgtcgtat tagcgggggc cggtcccgct aacgcgccct ccaaaaagct
     1981 agcgccgggc ccaatggatt gttgtccacc ttggacgtca ggtcgaagcg catggaggcc
     2041 cgccggacag ggtcgtactg gggccagccg caatccccac tgacggcaaa actcacccac
     2101 gcgcgatgca tcgcgtcggc gagcggttgc gggggatcac gtccgagcat cggctcggtt
     2161 ccgaggccca gagtatcgaa cacgaaggcg atttccatgc catgcgccgc cccgaggcga
     2221 ccgctcatct gcggcgagcg ccaggcaaac tcgtacatga aggtggcggc gcgggccttg
     2281 gcggcatggg catcagccat gcgaagagcc ggaatgcgcc agtaccagtc ggtctggatc
     2341 gccgagaaca ggtcgccggt gctggccccc ggataggcgg cgcgatatgc gttcaaaccc
     2401 tcggccgata agccgtaggc cgctgccaac cttgaaaggg attcctcggt gatgcggtct
     2461 atggagccgt cagagaacag gaacaatcga gtctcctcgg tattcgatcc gactagcagt
     2521 tccatgctcg cggctgcacc cgcgcggatg gcctcatcgg aggctccgga agggtctgtc
     2581 catcgaccgt gggcgcccat ggaaggccac tgagtgtaac ctcgccccag aacgccgggt
     2641 ccgggcgggc aagcagttca ttgcgcaata gcgcctgagc ctggagcacc cccgctcggg
     2701 cgcggttgcg gtaatcgcct ccctggaggc atcgacgtcc agaagctcgg cgagcctccg
     2761 tgccaatgcg ctccgcggtc gctggcgttc ggggtgttcc cgctttggac gatggcgcgc
     2821 cggaacagcc cccgggcgag tggcatagta aggaggttcg cgacgctcat gccggatagg
     2881 cgacctgagg cgatttcggc ccaaattcaa acgcatcgcg cacacactcc cagggctcaa
     2941 tcggttgcgg cggccgcaag cgattggcac cgaacggtgg cgcggcggag ggcaccccct
     3001 tgaacgcact caccccgtta ttgaagcggc cgcgcagcct gccacaatcg agcttgggaa
     3061 aagtccggaa tgggtcacgg gcagtaaaat tctcactgag tatatgttcc ccgaattgtc
     3121 ccagataacg gacattgtcg cgagcgccag caatcccaag actgcctgag acctataggg
     3181 ccggcaatgg tccctcccct gcccgctcac gttcgttcat cgcgcggtgt tctcgaaaaa
     3241 atcggttata ctcattaaac tactgcgcgc gagggcaggg acggagacgc gtgcttttct
     3301 cggttagcgc tgcttgcgcg agtgcgcgag gtcgatttcg ggaggtgctc ggacgctact
     3361 gccgtgcagc aattccggat ggttcgtcaa gaactcctgg atcgcatgct cgaccaaggt
     3421 ctgcactgtc acgccgcgca acacggcggc aagcttgagc tgaggctatt tcgcttcgga
     3481 aatgcgggcg ccctgggcgg tttagaccag gatcacgcgc gattttggcg cggccatgga
     3541 tgttattttg ttagttttct cggttccaat aatgacattc tttaaattca caatattgtt
     3601 gtcaagcttg ttgccgagct tagtcttcga acttccggtt ccgcaggtaa ttggacggcc
     3661 gttttcagcc ggcttttgat taacttcgtt tgatcattat caccgggcga atggcggttc
     3721 cctcgcgcaa aggtgacgac gagccgccga gtgacattaa attgtacctc gctgtgtctt
     3781 gccttcgatt ttccggaaaa ctcggcgcgg catgccggtg ctgccgccct gttccccctc
     3841 tcgatgcctc tcgatgtacg ggacggagat gcctcggcca ggattacggt cgggcgcctc
     3901 ccctgccctg ctccggccag tataatgggc tggagtatgt ggtgttgagc ggatgaaccg
     3961 tctccgatcg ccaggagttt cttcgcgtcg ctcgcttgct catgtcggcg atcgccggct
     4021 gttgccccga aggctacctt ttgccggggg cgatcctaat tgatgtgtga cgaacgcgat
     4081 gccgagcacg gttgctgtgt agcaggtgcc aaacatctcg gcagtcagac cgctgaccag
     4141 ccgtgcaacg ctaagctaca gcatgcccat ggcggccgcc gggccggtac tcgctcggcc
     4201 acaccgggaa catgcgctag gcagccacct cgaccgggag cccggcggct ttccattcgg
     4261 gataaccgtg cccgagccgg cgcgcccggt aaccctttgc tcgcaaagtg gcgactgctt
     4321 cgaacgcgag cacgcaatag gcgccgcgac aataggcaac gatctcgcgg tcgcggggca
     4381 actcggcgag gcgcctttcg agttcggcca aaggaacgct gagtgcaccg ggcagatggc
     4441 ccaggctgaa ttcttcctcg ggccgcacgt cgagcacgct caccagccca tcgcgcagcc
     4501 gcgccagcag atcctcccgc gacaccggtt cgaactcgtc gcggacccgg aaataggccg
     4561 tcatcacccg ttttcctctc ttctggtctc gtggcggaac cgtcggatat agctgcatgt
     4621 caggtaaggg gccaaaacag accgtgttcg ccgctctcgc cgagatcgcc aaggcgctcg
     4681 gccacgcgca tcggctcgag cttctcgagc agctgggcca gggggagcag agcgtggagg
     4741 atctcgctac gcgttccgat ctgacttttg ccaatacctc ccgccacctg cagaccctgc
     4801 ggcgcgctcg cctcgttgac gcgcggcgcc acggtaagcg tgtgctttac agcctggcct
     4861 cttatcgcag atcaccgtcg gtcgcgtgac gacgcatcat gcgctggctg ttgaaataat
     4921 atgcgatgtt gtcgatcgcc tgcttcatca tccagcggca ccgggaagga tcgtcatagc
     4981 aaagcgctag aacggcgggc tcgatcttgt cgtatgagcc cgacctgcga catagaggac
     5041 gccaagtcgg cgaccgcaac ggggctcagc cagcgccgac gagaagcagg ccggcgtccc
     5101 gggcgaaaaa tttatggtca cccctcgggc gaggcgcaat cctatagtga taaaggaaag
     5161 cggggcgaac catgaatagc gagcgggtca gttaccgctg tgctcgggag cggtgaagct
     5221 gcctccgcag cggttcctct gcctgtcgcg gttcctcgcg gaacccggct gccatttgcc
     5281 gcctcctttt attttcagat gtcgcggatg tgcggcagca ctttcttgtc gaatagttcg
     5341 agcgctttca tcagcgcgtc gggatgctcc gagcccgggg tctggaacga cagatcgacg
     5401 atgccggcgc ctatctgttc gcggcattgc ttcaattgcg ctgcatcata tccagcacca
     5461 caccggcgcc ggcagcaagg aggacacggc gagctgccgg gagttcttcc acgacatatg
     5521 ccggcgtggg ctgccggacc cgctgctggt ggcagtgacg gggcgccggg tatgatccgc
     5581 gcgatcgaag aatgcctgcc gcgcaccgcg cgtcaacgct gcctcgctca taaggtgcgg
     5641 aacttgcaaa gcaaggtacc cgaagacctc tggcctgagt tcaacgccag cgccgttcgg
     5701 ctgctaccag gcggcctcgc gggcgcgtca gtcaaggatt cccatttgtc cggttcttgt
     5761 cggatacctg cggcataggc ggagccgtgg gcagcaggcc cgctgcagca atgcgcgcac
     5821 gtacgggaca gggtcgggat cgccttccaa gccgcgcgcc cccgacgcaa gaggccgggc
     5881 gttgattcag atcaaggcgg gcggcatcgt cgcctcccta ccataccaac atgaaggctt
     5941 tccttgcgat cgcaacgttg atgcctgtct cggcctgggc ggcagagaca ccgaccgaac
     6001 cttacagctg ctgggggccg cagatgatgt ggggtgcggg atggtacggg atgatcttcg
     6061 ggcccctgtt catgatgctg gtacttggca tagtggtcgt gattggagcc ctcattgtgc
     6121 ggtcggttgt cgggccctgg cagcccgcgc cgcatgggcc gcctgtcgac cgccgcccag
     6181 ttgacatcct caagtagcgt ttcgcccgca gcgagatcga caagaacgag ttcgacgagc
     6241 gacggcgtgt gctcggagac taagcatgaa ggttctgttc atcctgaacg gcccgcccta
     6301 cgggagcgag acgagctaca atggcctccg attggcgctc gcattggcca agcgagaccc
     6361 gtcggatagc attacagtat tcttgatggc ggatgccatt gcctgcggga agaaagggca
     6421 gagaactccc gacggctatt acaacatcga acggatgttg aagcgcttca cgttgggaaa
     6481 tcaccgcgtt ctcttgtgcg gtacctgtat ggatgcgagg ggaatcagtg aggctgagtt
     6541 gatcgaaggt gcccggcgta gcaacatgga cgagctagcg gaagccaccg ccacggccga
     6601 tagattgctg gtgttctagg aaccatcagg ctaccggcag cccagctatc ttccactcag
     6661 gaaatccctc gagcgccgcg cgggcatcgt cggccgcgcg aggaaacagc ggttctcggg
     6721 cagtcgaggt tgttatgatc caccttggcc cacagaaaag cggactggtg ctcggtgcgt
     6781 tggtcggcgg atggcatctt ctttgggcca tcatcgttgc cgccggctgg gggcaggcgt
     6841 ttatcgattt catcctctgg atgcattttt taaagccggt attcgtgatc gaaccattcg
     6901 cgatcggccg ggcggttgtc ctgatcctcg taacaggggc tataggatat tttataggat
     6961 taatcggcgc cttgctgtgg aaccggctgc agactcgctg agctcttgtg cccgatcccg
     7021 cccgacacga tgcgcgcggt ctctccgagc ttctggcgga gcatgcgcgc accgtgagca
     7081 gcgagcgcgt ctcgctcgcc gagattgccg atttcctcgg cacgcgctcg atcggggcat
     7141 ggctgctgat cctggcattg cccatggtgc tgccggtgcc cgcacccggt atctcggtgt
     7201 tgtttggcat gccgctgatg atgatttccg cgcagctggc gctgggcgga cggcaggtgt
     7261 ggctgcccgc ctttatcctt cggcaatcac tggcacgcgc ggattacgtg gcgctggtgg
     7321 cgcgcatgcg gcccgcaatg gaacgtttca agcggcttgt gcgaccgagg gctttgtggc
     7381 ttgccaatga ttggacgaag atcccgatcg ggccgatctg tctggtcctc gggatgatca
     7441 tcacgctgcc catacctctg ggccatgtcg cgccgggtag cgcgatctgc ctgctggctc
     7501 tgggccttat ggaacgcgat ggcgttgtca tcggcatcgg gtttgtcgcc gcggtgctgg
     7561 ctcttgtgat cgtgaccgtt gcgtccgccg gcctgattgc cgtgcgcccc tggcttatca
     7621 ggtaaccgcc ggcgcctgcc ccgaccgctc ggggagcatc ggctggatga gcttcttggc
     7681 gagctcgagc agcaagagag caatcaagcc gctggccagt gcgacaagga tatcgtcgcc
     7741 gtgcagcggg ccgaagtgga acaattcccg cgccggggga aagaggatga cgccggcaag
     7801 gatcgctgcg gtcgccgcgc cgacccacca tagggccgca ttgggtcgtg tgaacgcagc
     7861 aaaaacggac gcgccaagcg accgattgac gaggacaagg ccgagattgg tggcgaccag
     7921 ggcggcgaag gaaagggcgc gtgcgtccgt ctccggcagt ttctgatgca gcgccatgac
     7981 gaagaggccg gccaccaggg ccaggacaag agctccttga aaaaggctcc aaaccgcaaa
     8041 gccgacagtg aatagcggcg cggatggatg acgtggcggc cggcgcatcg tgtcggcttc
     8101 ctcgccttcg gcttcgaaca cgatcgagca cacgggatcg atcaccattt cgagaaaggc
     8161 gatgtgaagg ggccagaaca ccagcggcaa atcgaagagc agcggtatca gggccaggcc
     8221 cgcgatcggc acatggacgg cgagaatata ggccatggcc ttgcgcagat tgtcgtagat
     8281 ccgccggccc agccggacgg cggtaacgat cgacgtaaaa tcgtcatcga gcaggatcaa
     8341 ggaagcggcc tcgcgcgcga cgtcggtgcc gcggccgccc atggcgatac cgatatgcgc
     8401 ggctttcagc gaaggcgcgt cattgacgcc atcacccgtc atggccacga cttcgccatt
     8461 ggccttgagc gcattgacga tccgcaattt ctgctcgggc atcgtgcggg cgaagataag
     8521 ggccgagcgc acgcgctctt taagatcggc atcgctcatg cgttcgagct cggcgccggt
     8581 gacgatatcg ccggcctcta gattggcctg cgcggcgatc gcgcgcgccg tttgcgggta
     8641 atcgccggtg atcatgatca cgcggatgcc ggcggcgcgg cactcctgga ccgccgccgg
     8701 cacggtcggg cgcaaggggt ctgcgaggcc cacaaggccg aggaattcga acggaaattc
     8761 gtgcggcgtc tctgggcgcg caaatccggc gggcacggat gcgcgcgcaa cggcgagcac
     8821 ccggatgcct ttctgcgcca tccgatcaac cgcgtggcgt agctccgccc gatcgcgttc
     8881 cggcaaatgg cagagctccg caatggcctc gggcgcgcct ttggcggcaa cgacgagctc
     8941 gggagagcca accccttcat agacatgggt gacggcaagt agatccggcc gcagcccgta
     9001 ttcccatttg agggtaaggt cggtgtgcag caggtgatcc ctcggcaggc gttcagtgcc
     9061 gaggg
//
LOCUS       DATLJT010000466         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1635219,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000466 DATLJT010000000
VERSION     DATLJT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635219"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..700
                     /locus_tag="VM910_10780"
     CDS             29..700
                     /locus_tag="VM910_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVI63052.1"
                     /translation="MTTISKTEQRGADASGKPGRDCPRCPRLRALREEWRHREPSWFN
                     APVASFGPRSARLLIVGLAPGLRGANRTGRPFTGDFAGGLLYDTLKRFGFARGSYLAA
                     ADDGLTLVDARITNAVRCVPPENKPTPAEIQTCRSFLQATIAEMRNLRAIVTLGRISH
                     DSTLAALKERRSAAPFGHGASHDIGRLRIFASYHCSRYNTSTGVLTPKMFEDVFAAAR
                     AFLGD"
     gene            complement(763..1329)
                     /locus_tag="VM910_10785"
     CDS             complement(763..1329)
                     /locus_tag="VM910_10785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI63053.1"
                     /translation="MTTLHDPTVLPADIPAPQDDGAARHLTGTRLPSLPLASTDGSQV
                     DLSKLAGRTVVYIYPRTGVPGQPLPDGWNQIPGARGCTPQSCGFRDHFAELKQLGVAR
                     LFGLSTQDTAYQREAVERLHLPFPVLSDQNLKLARAIKLPTFTTSGMTLLKRMALIID
                     DGVITKVFYPVFPPDKNAEEVVAWLRSA"
     gene            complement(1326..1757)
                     /locus_tag="VM910_10790"
     CDS             complement(1326..1757)
                     /locus_tag="VM910_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HVI63054.1"
                     /translation="MSSRDFPPQDILVPISVGELMDKITILEIKSERLKNPSQLDNVT
                     QELAALRAVRLGDVDRVMLDKLGAELRQVNAKLWDVEDAIRECDARGDFGQCFIDLAR
                     AVYRLNDERSRLKKAINLASGSRLVEEKSYSFYHSKGEDHP"
     gene            complement(1873..2277)
                     /locus_tag="VM910_10795"
     CDS             complement(1873..2277)
                     /locus_tag="VM910_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI63055.1"
                     /translation="MPLPVTFDHCVVHVSDWERSNAFYRDVLGAELVRRPQGWAYRFG
                     AAQLNLHGPGFHPAEVARLPVEPGNSDLCFRWNGPIADAITHLERCRVPIDAGPIERF
                     GAKGSGTSVYFRDPDGSLLEFISYHREDGDRS"
     gene            complement(2615..3466)
                     /locus_tag="VM910_10800"
     CDS             complement(2615..3466)
                     /locus_tag="VM910_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63056.1"
                     /translation="MKKAEEWAEQILFNYCDARGIYKRTYACRFDQFDAMAIDSLGQT
                     FPASRPLTMHDIGVSDARTACDFFHKLAARFPHLNYYASDYEPALMLLRSGKGGAVVT
                     LNKKGEAIEIVMPPFVFNLIKPENFLFYPINYAFFLFARAIVLRRTLAKYRAGKITPS
                     PLVLFCPEARDLAASDGRFHLLEYDLLKPHALPSRVDVVRVMNVLNSSYFDPQQLSIA
                     VGHIFESLTVGGLLIVGSNDEAGTQVRGGIYRKLDQGFQTLAKSGADHDAHQAIMSWT
                     ASERDCL"
     gene            3882..>4138
                     /locus_tag="VM910_10805"
     CDS             3882..>4138
                     /locus_tag="VM910_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63057.1"
                     /translation="MIEDPSAQRVVDLNHAILKSRGGIVSAQSMILDFGCGSGRHTYE
                     YLDAGFPNAFGYDVKNYVNLRSAADIAHFRFDPMPADRSDYP"
BASE COUNT          794 a         1286 c         1290 g          768 t
ORIGIN      
        1 cagcgggcgg gcgcgggcgc gcccctcgat gacgacgatt tcgaagactg aacaacgcgg
       61 cgccgacgcg tccggcaagc ccggccgcga ctgcccgcgc tgcccgcgcc tcagagcgct
      121 ccgcgaggag tggcgccatc gcgagccgag ctggttcaat gcgccggtcg cctcgttcgg
      181 tccgcggagt gcgcggctgc tgatcgtggg tttggccccc ggtctgagag gcgcaaaccg
      241 cacggggcgg ccgtttaccg gcgacttcgc cggcggcctc ctctacgaca cgctcaaacg
      301 tttcggcttt gcccgcggca gctacctggc cgcagcggac gatgggctta ccctcgtcga
      361 tgcgcgcatc accaatgcgg tgcgctgcgt gccgcccgaa aacaagccca cgccagccga
      421 gatccaaacc tgccgttcgt ttttgcaagc gaccatcgcc gagatgcgaa atctgcgcgc
      481 catcgtgacg ctcggacgga tttcccacga cagcacgctc gccgcgctca aggagcgccg
      541 ctcggcggcg ccgttcggcc atggcgcctc gcacgacatc ggccggttgc gcattttcgc
      601 cagctaccac tgctcgcgct acaacaccag caccggcgtg ctgacgccaa aaatgtttga
      661 agacgtgttc gcggcagcgc gcgccttcct cggcgattga atcaatggcc gctcgtcccg
      721 cgaaggcggg gcgtggtcga ccatcgtttc agctcacccg ccctatgcgc tgcgaagcca
      781 ggcgaccacc tcctcggcgt tcttgtccgg cgggaatacc ggatagaaca ccttggtgat
      841 gacgccgtcg tcgatgataa gcgccatgcg cttgagcagc gtcatccctg aggtcgtgaa
      901 ggtcggcagc ttgatcgcgc gtgcgagctt gaggttctga tccgaaagca ccggaaacgg
      961 cagatgcaga cgctcgaccg cctcgcgttg ataggcggtg tcctgggtcg agaggccgaa
     1021 aaggcgggcg acgccgagct gcttgagctc ggcgaaatgg tcgcggaagc cgcacgattg
     1081 cggcgtgcag ccgcgcgctc cggggatctg attccagccg tccggcaacg gctgcccggg
     1141 aacgccggtg cgggggtaga tgtagaccac cgtgcggccg gcaagtttcg aaagatcgac
     1201 ctgcgagccg tcggtcgacg cgagcggcaa ggacggaagt ctcgtgcccg tgagatgccg
     1261 cgctgcgccg tcgtcctgcg gtgccgggat gtcggcgggc aaaacggtgg gatcatgcaa
     1321 ggtggtcatg ggtggtcttc gcctttggaa tgatagaacg aataggactt ctcctcgacc
     1381 aggcgcgacc ctgacgcgag gttgatcgcc ttcttgagcc gcgagcgttc gtcgttgagc
     1441 cggtagaccg cgcgcgcaag gtcgatgaag cactggccga agtcaccgcg cgcgtcgcat
     1501 tcgcggatcg cgtcctcgac gtcccaaagt tttgcattca cctgcctgag ctcggcgccg
     1561 agcttatcga gcatgacacg gtcgacgtcg ccgagacgca cggcacgcag agctgcgagt
     1621 tcctgcgtga cattgtcgag ctggctcggg ttcttgagcc gctcggactt tatttcgagg
     1681 atggtgatct tgtccatcag ttcgcccacc gagatcggca ccaggatgtc ttgcggcgga
     1741 aagtctcgag agctcatgtg gcgccctcgg cctttgccgt cgcatgcggc gctcgcagcg
     1801 ccatccatgg cgacgaggtc gtcgtccggc aatgccccgc ggttggcggg cggcttgggt
     1861 ggtaggcgat cgtcagctgc ggtctccatc ctcgcggtga taggaaatga attccaacag
     1921 cgaaccgtcg gggtcgcgga aatagacgct tgtgcctgac cccttggcgc cgaagcgctc
     1981 gatcggccca gcgtcgatcg ggacacggca gcgctcaagg tgggtgatgg cgtccgcaat
     2041 cggcccgttc catcggaagc agagatcgct gttgccaggc tcgaccggaa gccgcgccac
     2101 ctcggccggg tgaaagcccg gcccatgcaa attgagctgg gcggcgccga accggtaggc
     2161 ccagccctgc ggacgccgaa cgagctcggc gccgagcaca tcgcggtaga acgcgttcga
     2221 gcgctcccag tccgagacat ggaccacgca gtggtcgaag gtgacgggta atggcatggg
     2281 cactcctcgt cgaccggcgg cgcaggcggg gccgaaaggc agcgccggcc tgcgggggat
     2341 gagcccccgc gggcccagta gcttaggcca atacagacgc gtcgatccag cgatgtcgcc
     2401 gagggccacc caagcccctc gatcctgcgg caccatggcg gccttgccgg tcttgccatc
     2461 gggtgtgcca gcggcagcca gccagccgcg ttcttgtctc caccggtcgc aacgagattg
     2521 accgcgaccg cgccgctcgc gaactcagcg ggcacatgcc cgctcatgac tcccaacggc
     2581 atgtcggcgg cggcgaaatg gaccgcacta gacatcacag gcagtcgcgt tcgctcgcag
     2641 tccagctcat gatggcctgg tgagcatcgt gatcggcgcc tgacttggcc agcgtttgaa
     2701 acccttggtc aagcttgcga tagattcccc cgcgcacttg ggtgcctgcc tcgtcattgg
     2761 agccaacgat cagcagcccg ccgacggtca aagattcgaa aatgtgtccc accgcgatcg
     2821 acagctgctg tggatcaaaa taggaagaat taagaacgtt cataacccgc acgacgtcca
     2881 ctctcgacgg cagggcgtga ggctttagca gatcgtactc cagcagatgg aatcgtccat
     2941 cgcttgcagc aaggtctcgc gcttccgggc agaacagcac cagaggtgac ggcgtgatct
     3001 tgccagcgcg atattttgcc agcgtccgac gaagcacgat cgcacgcgca aacaaaaaga
     3061 aggcataatt gattggatag aacaagaagt tctctggctt gatcaaattg aatacgaaag
     3121 gcggcatgac gatctcgatc gcctctcctt ttttgtttag cgtcacaacg gccccgccct
     3181 tgccggaacg caacagcatc agcgcaggct cgtagtccga agcgtaatag ttcagatgcg
     3241 gaaaccgcgc tgcgagctta tggaagaaat cgcacgccgt gcgggcatcg ctcacgccga
     3301 tatcgtgcat cgtcaatggg cggcttgcgg ggaacgtttg cccgagggag tcgattgcca
     3361 tcgcgtcaaa ctgatcgaac cggcacgcat acgttctctt gtagataccg cgagcgtcgc
     3421 agtagttaaa aaggatttgt tcggcccatt cttcggcctt tttcatttca tgtagcttct
     3481 cgtaaagtgt aatcgaaacc cgccgtgttc caccaaaaaa acctctgatc ttcgcaggcg
     3541 atgaaagctg ctcgaggcga taaagtccgg tttttatcat cgcatgccca tgcggctcac
     3601 cctacccggc cagttgataa aggcatctcg gcgatgcggc cggattggga attggcagtt
     3661 gcgtcgatca acttgccgac tcgatcatct gccttcgagt aggagatcaa attgtacgga
     3721 ctctctgcct cgccctggcg attgagcggc aaaaaatgcg cgggatgcca agcgcacgcg
     3781 gtgcattcct gcggatttgc gccacacaat cgaaacggcg catggctgtc aactgcgaaa
     3841 acttgcacgc ggctcgtcga cgtacgtaag atgacgacgc gatgatcgaa gacccgtccg
     3901 ctcagcgcgt cgttgacctc aaccacgcga ttctcaagtc gcgcggtggc atcgtgtcag
     3961 cgcaaagcat gattctggat ttcggatgcg gttctggccg gcacacctac gaatatctcg
     4021 atgcgggatt tcccaatgcc ttcggttatg acgtcaagaa ttatgtcaat ctgcggtctg
     4081 ccgctgacat cgcgcatttc cgcttcgatc caatgcccgc agacaggagc gactatcc
//
LOCUS       DATLJT010000467         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_27309,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000467 DATLJT010000000
VERSION     DATLJT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27309"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VM910_10810"
     CDS             complement(<1..772)
                     /locus_tag="VM910_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI63058.1"
                     /translation="MKAGLPTTSVAEALAEEIAAVKPGMLPDTTRRKCQDLLIDVVGL
                     CITARNEGYITSALGGCDDDGVCTAIGHRRLLTAAGAAFVNGTAAHGEDFDDTFEGGP
                     VHAGAVVVPAVLAACERHNPDGRMALIGIAIGTEVLCRLSLVVPKAVHRAGFHPTAIF
                     GAMGAAAGVGAALGLQAREIVDALGIAGSMAGGIIEYLAEGAWTKRLHAGWVAQSGLR
                     AALLARSGFVGPRTVFEGTHGLFHGFAHTTQGDYAALTG"
     gene            complement(785..1849)
                     /locus_tag="VM910_10815"
     CDS             complement(785..1849)
                     /locus_tag="VM910_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI63059.1"
                     /translation="MKHRRVSRREMLKGTAALLAGSALSTRVLAAAPPPEPVTQALID
                     AAKKEGQVNYYTSTDLPVAEKVAKAFEAKYPGIAVRVERSGAERVFQRIGQEYASNIH
                     AVDVVNSSDAAHFIVWKRDGILAPYVPEDVARYPAEHRDVDGQFASFRVWLSIIAYNT
                     NLVKPEDAPKSFADLLDPKWKGKIVKAHPGYSGTIMTATYQMQRDLGWSYFEKLAKQN
                     IMQVQSSADPPKKLDLGERAVMADGNEYNIFQLKEAGRPVEPVYATEGSPLIIGPNGI
                     FKSSPNPNAAKLFQSFCLGREAQQLIIDVGGLRSVHPDTKEKAGRKPLTEIKTMRDDA
                     AAVEKESEAIKARYSKIFHV"
BASE COUNT          367 a          639 c          614 g          387 t
ORIGIN      
        1 cgccggtcaa cgcggcgtag tcgccttgcg tcgtatgcgc aaagccatga aacagcccgt
       61 gcgtgccctc aaataccgtg cgcgggccga caaatcccga ccgcgccaga agcgccgcgc
      121 gcaagcccga ctgcgccacc cagcccgcat gcagccgctt ggtccaggcg ccttcggcca
      181 gatattcgat gatgccgccg gccatgctgc cggcaatgcc gagcgcgtca acgatctctc
      241 gggcctgcag tccaagtgcg gcgccgaccc ccgctgccgc tcccatggca ccgaagattg
      301 cggttggatg gaagcccgcc ctgtggacgg ccttgggcac cacaaggctc aagcggcaca
      361 aaacctcggt accgatcgct attccgatca acgccatgcg gccatcggga ttgtggcgct
      421 cgcacgcggc cagcaccgcc ggcacgacca ccgcgccggc atgcacggga ccgccttcaa
      481 aggtgtcgtc gaaatcttca ccgtgggcgg ccgtgccatt gacgaaagcc gcgcccgcgg
      541 cggtcaacag gcgacgatgg ccgatcgcgg tgcagacacc atcgtcatcg cagccgccaa
      601 gcgcgctcgt gatatagccc tcgttgcgcg cggtaatgca gagcccaacc acatcgatca
      661 ggaggtcctg acatttgcgc cgtgtggtgt caggcagcat gcccggcttc accgcggcga
      721 tttcctcagc cagcgcttcg gcgacggacg tggtcggcag accggctttc atgatccgca
      781 gacgtcagac atggaaaatc ttggaatagc gcgccttgat cgcctcgctc tccttctcga
      841 cggcggcggc atcgtcgcgc atggtcttga tctccgtcag cggtttgcga cctgccttct
      901 ccttcgtatc cggatgcacc gagcgcaggc cgccgacatc gatgatgagt tgctgcgcct
      961 cgcgcccgag acagaacgac tgaaacagct tggcggcatt cggatttggc gacgacttga
     1021 agatgccgtt cggaccgatg atcagcggcg agccttcggt ggcatagacc ggctcgaccg
     1081 ggcggccggc ctccttcagt tggaagatgt tgtactcgtt gccgtctgcc atgacggcac
     1141 gctcgccaag atcgagcttt tttggcggat ccgcagacga ctgcacctgc atgatgtttt
     1201 gcttggcaag cttctcgaaa tagctccagc cgagatcgcg ctgcatctga taggtcgcgg
     1261 tcatgatggt gccgctgtag ccgggatgcg ctttgacgat cttgcccttc catttcgggt
     1321 cgagcagatc ggcgaaactt ttgggtgcgt cttccggttt cacgagattg gtgttgtagg
     1381 cgatgatcga gagccagacc cgaaagctcg cgaactggcc atcgacgtcg cggtgttctg
     1441 cgggatagcg cgccacgtcc tcaggcacat agggagccag gatgccgtcg cgtttccaga
     1501 cgatgaaatg cgcggcatcg gaggagttga ccacatcgac cgcgtgaata ttggacgcat
     1561 attcctggcc gatacgctga aaaacgcgct cggcgccgct ccgctccacc cggaccgcaa
     1621 tgccgggata ctttgcctcg aatgcctttg cgaccttttc ggcaaccgga aggtctgtcg
     1681 aggtgtagta gttgacctgc ccctccttct tggccgcatc gatcaaggcc tgcgtaaccg
     1741 gctcgggcgg tggcgcggcc gcgagcacac gggtcgagag cgccgacccc gcaagcaaag
     1801 cagcggtacc cttgagcatc tcgcgacgcg agacccggcg atgtttcatg gcgtttccgt
     1861 ctgtttggcg atgtcaggac accgcaagct atcttttatt cctacgccgt aaacctagtc
     1921 tatcgtcgac agtcggtttg cgttgcagct aaattgccgg gcaaagaacg gtgtgggaga
     1981 cgacgatggc cggcccaagg tttgcgc
//
LOCUS       DATLJT010000468         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1176263,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000468 DATLJT010000000
VERSION     DATLJT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176263"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1536
                     /locus_tag="VM910_10820"
     CDS             <1..>1536
                     /locus_tag="VM910_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI63060.1"
                     /translation="AVDPEYTLSQATSLARQHTALGVPLLRENELIGVIVLARQRVEL
                     FTEKQIALVTTFADQAVIAIENVRLFDEVQAKTRDLEESLQQQTATSEVLQVISSSPG
                     DLAPVFDKMLENATRVCGAEFGTMMLLEDGVVRPVARYNSPPALAAVRGGEAYRPPPG
                     SLLATAIETKNLAQIADLQTSPTYIERARPTVDLVELGGARTVVVVPMLRDNEVIGTI
                     TIYRQEVRPFDGKQIELVGNFAKQAVIAIENARLLRELRQRTADLSEALQFQTASAEV
                     LKVISRSPDALQPVFDVIVETSRELCRAQASTIFTLRDGRFYVAAFAGAQTELLQYMK
                     DHPIPIEQKGSALARSVREKRTVHIPNTAEDPEFAEGSVIGMGEPRATLTVPLTRDGE
                     VIGGITLRQSHLTPFTARQIEAIETFADQAVIAIANVGLFEQVQERTNDLSESLQQQT
                     ATADVLKVISRSSVDLETVLDTLVETVARLCRADQVYMFHLRHDLWHLIAACGLSAEA
                     REF"
BASE COUNT          317 a          473 c          452 g          294 t
ORIGIN      
        1 ggcggtcgac ccagaataca cgctgagcca ggccacttcg ctcgcccgac aacatacggc
       61 cctcggcgtc ccgttgctgc gcgagaacga gctaatcggc gtcattgtac tcgcgcgcca
      121 gcgcgtcgag ctttttactg aaaagcaaat agccctggtt accactttcg ccgaccaggc
      181 tgtgatcgcc atcgagaatg ttcgcctgtt cgacgaggta caggccaaga cccgcgatct
      241 cgaggaatcc cttcaacaac agaccgcaac gtctgaagtg ctgcaggtca tcagttcctc
      301 gcccggcgat ctggctccgg tcttcgacaa gatgctggaa aacgcaacac gggtttgcgg
      361 tgccgagttc ggaacgatga tgctgctcga ggacggcgtg gtgcgcccgg tcgcgcgcta
      421 taatagcccg ccagcgcttg ccgcggtgcg aggtggtgag gcgtatcgtc cacctccggg
      481 aagcttgctg gcgacggcga tcgagaccaa gaacttggct caaatcgcgg acctgcaaac
      541 cagtcccacc tacatcgaac gtgcccgacc tactgtcgac ttggtggagc ttggcggcgc
      601 acgcaccgtt gttgtcgtgc ctatgctgcg ggacaacgag gtgatcggca cgatcaccat
      661 ctatcgccag gaggtccgtc cgttcgatgg caaacagatc gaactggtag gcaatttcgc
      721 caaacaggcc gttatcgcga tcgaaaacgc gagactgctt cgggagttgc gccagcgtac
      781 ggccgatttg agcgaagccc tgcagttcca gacggccagc gccgaggtcc tcaaggtgat
      841 cagtcgctcg cccgacgcgt tgcagccggt cttcgatgtt atcgtcgaga catcccgcga
      901 gttgtgtcgt gcccaagctt ccacgatctt caccttgcgc gatggtcggt tttatgtcgc
      961 cgcctttgcg ggagcgcaga ccgaattgtt gcagtacatg aaagatcacc cgatccccat
     1021 tgaacaaaaa ggctcggcgc ttgcgcgatc cgtgcgggaa aagcgtaccg ttcacatccc
     1081 gaatacggct gaagaccccg agtttgccga gggaagcgtc atcggtatgg gcgaaccgcg
     1141 agcaaccctc acggtcccgc tcacgcgcga cggcgaggtg atcggcggca ttacgctccg
     1201 acaatcacac ctgacaccat tcaccgctcg gcaaatcgag gcgatcgaaa cttttgccga
     1261 tcaagcggtg atcgccatcg caaatgtcgg tctcttcgag caggtgcagg aacgcacgaa
     1321 tgacctgagc gaatccctgc agcaacagac ggcaacggcc gacgtgctca aggtcattag
     1381 ccgctcctct gtcgacctcg agacggtgct cgacacgctg gtggagaccg tggcgcgtct
     1441 ctgccgcgca gaccaggtat atatgttcca cctgcgccat gacctgtggc acctgatcgc
     1501 ggcctgcggg ctttcggcgg aggcaagaga attctt
//
LOCUS       DATLJT010000469         4519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3932862,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000469 DATLJT010000000
VERSION     DATLJT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932862"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VM910_10825"
     CDS             <1..600
                     /locus_tag="VM910_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63061.1"
                     /translation="ANPDYIDKVLKQLSLWDKRDDKIRMLSGGMKRRLMIAKALAHEP
                     RILFLDEPTAGVDVELRKDMWQLVRSLRASGVTIILTTHYIEEAEEMADRVGVINHGE
                     IILVEDKAELMRKLGKKRLTLELRDRLVAVPAALDSYGLTLGADGSELTYTYDTKGEH
                     TRITALLDDLQRAGIRFKDLRTTQSSLEDIFVGLVRERR"
     gene            597..1358
                     /locus_tag="VM910_10830"
     CDS             597..1358
                     /locus_tag="VM910_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63062.1"
                     /translation="MNLYAVRAIYMYEMARTGRTLFQSIVSPVISTSLYFIVFGAAIG
                     SRITQIEGVNYGSFIVPGLIMLMLLTQSVTNAAFGIYFPRFVGTIYEMLSAPVSPFEI
                     VLGYVGAAASKSIILGTIILATSGLFVPIHIVHPAWMVAFLVLTAVSFSLFGFILGLW
                     ADNFEKLQIVPLLIITPLTFLGGTFYPTNVLPPFWRTVTLFNPLVYLISGFRWSFFDI
                     ADVHVGWSVAMTLGFLALCMAIVSWFFKTGYRLKN"
     gene            complement(1396..>4519)
                     /locus_tag="VM910_10835"
     CDS             complement(1396..>4519)
                     /locus_tag="VM910_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI63063.1"
                     /translation="RQVTVMFSDLVGSTALAAQMDPEDLRELISAYHKCVAETVRRFG
                     GFVAQYLGDGVLVYFGYPEAHEDDAERAVRAGLEVIVALGAIKTPALLQTRVGIATGL
                     VVVGDLTGLGEGRESAIIGETPNLAARLQGIAEPNSVVIAEATRRLLGNLFELRDLGT
                     RELKGLAKPVRAWLALRESTVESRFEALHPSGLTALVGRDEETELLLRRWSRAKGGNG
                     QVVLISGEPGIGKSRLTAALLEAVGPEPHTRLRYFCSPQHTDSAFYPIIGQMERAAGF
                     LYNDTPQQKLDKLDAPLAETSTSIEDAVLIAEMLSLPNDGRYPALELAPQQRRQKTME
                     ALTTQIETLSRQKPVMMILEDAHWADPTSLEAFGRAIDRIRRLKVLLLVTFRPEFDVP
                     WVARPYVTALIVNRLAEHEAGAIIDRIAGNKPLSAGIRQDIIERSDGIPLFVEEMTKA
                     VLEAGGEIAAERAIAALPSRALAVPASLHASLMARLDRLGGPAKELAQIAAAIGREFS
                     HPLLASVVRQPEAELESALNRLVVAGLLFRRDEPPHATYLFKHALVQDAAYGTLLREP
                     RRTLHAGIAETIESEFADIAENRPELLARHWTEAGVIDKAAFLWGKAGQRSLARSALV
                     EAAEQLRRALDQIATLPSTPVLRGEQIKLQIALVNALMHVKGYAAPEPKAAVEQARQF
                     IERAEALGEPPEDPLLLFSALYGVWAASYVAFNGDVTRELASQFVALAEKQDQVVPLM
                     IGHRLIANSLMLTGDIAKGREHYNQAFAFYDPAKHRSLATRFGQDVGVSIFVYRALAH
                     WMLGYPDKALADANHALEDARAGGQAGTLMYAQFHTSLTNVLCAKYAAAVAESDEVVR
                     LADEKNAAIWKALATMQKGCVLALSGKAPEGIKMITSGITTYKSTGSRVYLPIFLAHL
                     SRAYAEVGQFDDAWYSIGEALSAVEATKERWYEAEINRISGEIALKLPQLGPSQAEAY
                     FERALTIARAQHAKSWELRAAMSAARFWRDRGMRDKARALLAPICGWFTEGFETHDLK
                     QANALLAALS"
BASE COUNT          863 a         1392 c         1322 g          942 t
ORIGIN      
        1 gccaacccgg attacatcga caaggtgctc aagcagcttt cgctgtggga caagcgggac
       61 gacaagatcc gcatgctgtc cggcggcatg aagcgccggc tgatgatcgc caaggcgctg
      121 gcgcacgagc cgcggatcct gtttctcgac gagcccaccg ccggggtcga cgtcgagttg
      181 cgcaaggata tgtggcagct ggtgcgctcg ctgcgggcgt ccggtgtcac catcatcctc
      241 accacccatt acatcgaaga agcggaggaa atggccgatc gcgtcggcgt catcaatcat
      301 ggcgagatca tcctggtcga agacaaggcc gagctcatgc gcaaactcgg caagaagcga
      361 ttgacgctgg agctgcgcga tcggttggtc gcggtgccgg cggcgctcga cagttatggc
      421 cttacgctgg gcgccgacgg cagcgaactg acttacacct acgacaccaa gggcgagcac
      481 acccgcatca ccgcgctgct cgacgatctg cagcgcgccg gcattcgttt taaggatttg
      541 cggacgacac aaagctcgct tgaagacatc ttcgtcggtc tggtgaggga acggcgatga
      601 acctctacgc cgtacgggcc atctatatgt atgaaatggc gcggaccggc cgcacgctgt
      661 ttcaaagcat cgtctcgccg gtaatctcga cctcgcttta tttcatcgtg ttcggcgccg
      721 cgatcggctc gcgcatcacc cagatcgagg gcgtcaatta cggctcgttc atcgtgccgg
      781 gcttgatcat gctgatgctg ttgacgcaaa gcgtcaccaa cgccgcgttc ggcatctatt
      841 tcccgcgctt cgtcggcacg atctatgaga tgctctcggc gccggtatcg ccgttcgaga
      901 tcgtgctcgg ttatgtcggc gcggcggcga gcaaatcaat cattctcggc accatcattc
      961 ttgccacgtc cggattattc gtgccgatcc atatcgtgca cccggcctgg atggtggctt
     1021 tcctggtgct gaccgcagtc agtttcagcc tgtttggctt tattctcggg ctgtgggccg
     1081 acaatttcga gaagctgcag atcgtgccgc tcttgatcat cacgcctttg accttcctcg
     1141 gcggaacgtt ttatcccacc aacgtcttac cgccgttctg gcgcacggtc acgctgttca
     1201 atccgttggt ctatttgatt tcgggcttcc gctggagctt tttcgacatc gccgacgtgc
     1261 atgtcggatg gagcgtggcg atgacacttg gcttcctcgc cctgtgcatg gcgatcgtgt
     1321 cctggttctt caagaccggc tatcgactga agaattagcc cattcgcggc tcacgccatc
     1381 aattcaaccg caccgtcaag acaacgcggc cagcaatgcg tttgcttgtt ttaggtcgtg
     1441 cgtttcgaaa ccctcggtga accagccgca tattggagca agaagagccc tcgctttatc
     1501 ccgcatgccc cgatcgcgcc aaaagcgcgc ggcgctcatc gcagcacgca gttcccagga
     1561 cttcgcatgc tgcgcacggg caattgtgag cgcgcgctca aaatatgctt ccgcttgcga
     1621 aggaccgagc tgcggcagct tcagcgcgat ttcgccgctg atgcgattga tctctgcctc
     1681 gtaccaacgc tctttggttg cttccacagc actcaatgcc tcgccaatgg agtaccaagc
     1741 atcatcgaat tggccgactt cggcataggc cctggacaag tgtgccaaga agattggcaa
     1801 atacactctt gagccagtcg atttatacgt ggtgatgccg gaggtgatca tcttgattcc
     1861 ctccggagcc ttgccggaca gcgccagtac gcagcctttt tgcatcgtcg ccaatgcctt
     1921 ccatatggcc gcattctttt cgtcggccaa tcggacgact tcatcggatt ccgcaactgc
     1981 tgccgcgtac tttgcgcaga gaacattcgt caatgatgtg tggaactgtg catacattaa
     2041 agtgcctgcc tggccgcccg cgcgtgcgtc ctcgagcgcg tgattggcgt ccgccagcgc
     2101 tttgtcgggg tagccgagca tccaatgtgc caatgccctg tagacaaaga tcgatacacc
     2161 gacgtcttgg ccaaaacgcg tcgcgagcga ccggtgcttg gcaggatcgt aaaatgcgaa
     2221 cgcctgattg taatgctctc ggcccttggc gatgtctccg gtaagcatca gtgaattagc
     2281 tatcaatcga tggccgatca tcagcggaac gacttggtct tgcttttccg caagcgccac
     2341 aaattgcgat gcaagctcgc gcgtaacgtc cccgttgaac gccacatagc ttgccgccca
     2401 gacgccatag agagcagaga acaacagtag cggatcttcg ggaggttctc cgagcgcttc
     2461 cgcgcgctcg atgaattgac gcgcctgttc aaccgcggct ttgggctcag gcgccgcata
     2521 acctttgacg tgcatgagag cgttgaccag cgcgatctgc agcttgatct gctcgcctcg
     2581 cagcaccggc gtgctaggta aagtcgcaat ctggtcgagg gcgcgcctga gctgctcggc
     2641 ggcttcgacc aatgccgaac gcgccagcga gcgctgtccc gccttacccc aaaggaacgc
     2701 ggccttatcg ataacgccgg cctcggtcca gtgcctcgcc aacaattccg gtctgttctc
     2761 ggcgatgtcg gcgaattcac tctcgatggt ttcggcgatg ccggcatgta atgtgcgtcg
     2821 tggctcacgc agcagcgtgc cgtaagccgc gtcctgcacg agcgcgtgct tgaacaaata
     2881 ggtggcatgc gggggctcgt cgcgacggaa cagcaagcca gccacaacga ggcgattcaa
     2941 cgctgattcc agttcggcct ccggctggcg cacgaccgac gctagcaagg gatgcgaaaa
     3001 ctcgcggccg attgcggccg cgatctgcgc cagctctttg gccggaccgc cgagccggtc
     3061 gagccgcgcc atcagtgagg catgcaggct tgcgggcacg gctagtgccc gggacggcag
     3121 cgcggcaatc gcgcgctcgg cggcaatctc accgcctgcc tcgagcaccg ctttggtcat
     3181 ttcctctacg aacaagggaa tcccgtcgct acgttcgatg atgtcttggc gaatgcccgc
     3241 cgatagcggc ttgttgccgg cgatccggtc gataatggcg ccggcttcgt gctccgcgag
     3301 tcgattgacg atcagggccg tgacgtaagg ccgcgccacc cacggcacgt cgaattcggg
     3361 gcggaacgtc accaacaaga ggaccttgag tctgcgaatt cggtctatcg cccgaccgaa
     3421 cgcttccaag ctggttgggt cggcccaatg cgcatcctcc aagatcatca tcacaggctt
     3481 ttgccgcgac agtgtctcta tctgtgtggt gagcgcctcc atggtttttt gccggcgctg
     3541 ctgtggcgcc agctcgagtg cgggataccg gccatcgttc ggcagcgaaa gcatctccgc
     3601 aatgagcacg gcgtcttcga tcgaggtcga agtctccgcg agcggagcat cgagcttgtc
     3661 gagtttctgc tggggcgtat cgttgtataa gaatccagcg gcacgttcca tttgaccgat
     3721 gatcggataa aacgcgctgt cggtgtgctg gggcgagcag aaatagcgca aacgggtatg
     3781 cggttctgga ccgacagctt ccagaagggc ggcggtgagc cgggatttgc cgatgcccgg
     3841 ttcgccggaa atcagcacta cctgaccgtt gccgcccttc gcccgcgacc agcgccgtaa
     3901 caacagttcg gtttcttcat cgcgcccgac gagggcggtc agaccgctcg gatgcagtgc
     3961 ctcgaaccgg ctttcgacag tgctctcccg cagcgccaac caggcgcgca cgggcttggc
     4021 aagaccttta agctctctgg tgccgaggtc ccttagctca aagagattgc caaggagccg
     4081 gcgggttgct tcggcaatga caaccgaatt cggctcagca attccctgca ggcgcgcagc
     4141 gaggtttggc gtctcgccga tgatcgcgct ttcgcgtcct tcgcccagtc cagtgagatc
     4201 gccgacgacg accagcccgg tggcgatacc aacacgagtt tgcaacaaag ccggcgtttt
     4261 tatcgcgccc agggctacga tcacctcgag gcccgcgcgc accgctcgct cggcgtcatc
     4321 ctcgtgggcc tcggggtagc caaagtacac aagcacgcca tcgcccaaat attgcgccac
     4381 gaaaccgccg aaacggcgca cagtttcagc aacgcatttg tgataggccg agatgagctc
     4441 ccgcaggtct tcgggatcca tctgagcggc gagcgcggtc gaaccaacca gatccgagaa
     4501 catcaccgtt acttgccgg
//
LOCUS       DATLJT010000470         3325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3014040,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000470 DATLJT010000000
VERSION     DATLJT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014040"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VM910_10840"
     CDS             complement(<1..619)
                     /locus_tag="VM910_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVI63064.1"
                     /translation="MHPAVLNGSYDVERVREDFPALAVKVYGKPLVYLDNAASAQKPN
                     AVLDRMAEAYKTEYANVHRGLHYLANAATEAYEGARTKVAKFLSARRSEEIIFTRNVT
                     EAINLVASSWGEPNIKAGDEIVLSIMEHHSNIVPWHFLRERHGAVIKWAPVDDEGNFL
                     IEEFEKLLTARTRLVAITQMSNALGTRVPVKEVVKIAHARGIPVLV"
     gene            complement(622..1926)
                     /gene="sufD"
                     /locus_tag="VM910_10845"
     CDS             complement(622..1926)
                     /gene="sufD"
                     /locus_tag="VM910_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVI63065.1"
                     /translation="MNVALVKTETGRAADRLFAAARDRLPGSGKVADARRRAFEAYEA
                     LGLPHRRIEEWKYTDLRALVREVLPLADAPDAAALERAQAALKACAIEGAAKLVLVDG
                     VFARELSDEADAGVSVRTLREALENGGVALLEANASDVMISLNAALATDGVVVSVADG
                     VSLTRPIQIVHVATAASASIVTRSHFKLGSAARATLVESFVAAEGAKAYQANDTLIIS
                     VGDEAELSQVRLMADAADAVNVTSEIFTVGAGTKLNLFNLTSGGGVSRFQGFVTLAGE
                     GTELTVNGVNLLNGTQHGDTTMVVDHAVPECVSREMFRAVVDDRAHSVFQGRIIVRPD
                     AQKTDGKMMTRALLLSDEAEADNKPELEIFADDVACGHGATAGALDDNLLFYLRARGL
                     PEKEAQALLIQAFVGEAIEGIADEALREVAMNQAQLWLEARG"
     gene            complement(1945..2697)
                     /gene="sufC"
                     /locus_tag="VM910_10850"
     CDS             complement(1945..2697)
                     /gene="sufC"
                     /locus_tag="VM910_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVI63066.1"
                     /translation="MALLEVRDLQVRVEDREILHGLSLSVNKGEVHAIMGPNGSGKST
                     LSHVIAGKPGYEVTGGQILFNGEDLLEMAPDERAAKGVFLAFQYPVEIPGVATMNFLR
                     TALNAQRKARGESEFSTPDFLKKVREVAKSLNVPQDMLKRGVNVGFSGGEKKRNEILQ
                     MALFEPALCILDEMDSGLDIDALRIAADGVNALRSPDRAIVVITHYQRLLNYIIPDFV
                     HVMLRGRVVKSGGKELALELEESGYAQFEEAA"
     gene            complement(2750..>3325)
                     /locus_tag="VM910_10855"
     CDS             complement(2750..>3325)
                     /locus_tag="VM910_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HVI63067.1"
                     /translation="GDCRGKNSKISWTQVETGSAITWKYPSCILRGDNSRGEFYSIAI
                     SNGFQQVDSGTKMLHLGKNTTSRIISKGIAAGKSQNTYRGLVTAHRKALGARNFTACD
                     SLLIGDKCGAHTVPYIEAKNSSATFEHEATTSKISEDVLFYCVQRGLSQEEAVGLVVN
                     GFVKDVLQQLPMEFAVEAQKLISISLEGSVG"
BASE COUNT          620 a         1071 c          978 g          656 t
ORIGIN      
        1 cgaccagcac cggaatgccg cgggcatggg cgatcttcac gacctccttg accggcaccc
       61 ttgtgccgag cgcattcgac atctgggtga tcgcgaccag cctggtgcgc gcggtcagga
      121 gtttctcgaa ctcctcgatc aagaaattgc cttcgtcatc gaccggcgcc catttgatca
      181 cggcgccgtg gcgttccctg aggaaatgcc agggcacgat gttggagtgg tgctccatga
      241 tcgagagcac gatctcgtcg ccggccttga tatttggttc gccccatgac gaggccacga
      301 gattgatcgc ctcagtcacg ttgcgggtga agatgatttc ctcgctgcgc cgcgcactaa
      361 ggaatttcgc aaccttggtt cgagcgccct cataagcctc ggtcgcggca ttggcgaggt
      421 agtgcaggcc gcgatgcaca ttggcgtact cggtcttgta ggcttccgcc atgcggtcga
      481 gcaccgcgtt gggcttttgc gctgacgccg cgttatcgag ataaaccagc ggtttgccat
      541 agaccttcac ggcgagcgcc ggaaaatcct cgcgaacgcg ctctacatca taggagccgt
      601 tcagaaccgc cggatgcatg gtcaacccct cgcctccagc cagagctgcg cctggttcat
      661 cgccacctcg cgcaaggcct catccgcgat gccttcgatc gcctcaccga caaaagcctg
      721 gatcagaagc gcctgtgcct ccttctccgg caagccgcgt gcgcgcagat aaaacaaaag
      781 attgtcgtca agcgcgcctg cggtcgcacc gtggccgcag gcgacgtcgt cggcaaaaat
      841 ctcaagctcc ggcttgttgt cggcttccgc ctcgtcggag agcagcagcg cccgcgtcat
      901 catcttgccg tccgtcttct gcgcatcggg acgcacgatg atgcgaccct ggaacaccga
      961 atgggcgcgg tcgtcaacca cggcacggaa catctcgcgg ctcacgcatt ccggtacggc
     1021 atgatccacg accatcgtgg tgtcgccatg ctgggtgccg ttcagcaggt tcacaccgtt
     1081 gacggtgagt tccgttcctt cgccggccag cgtcacaaat ccctgaaacc ggctgacccc
     1141 gccgccgctg gtcagattga acagattgag cttcgtcccg gcgccgaccg tgaaaatctc
     1201 ggaggtgacg ttcaccgcat ctgccgcatc ggccatcagc cgcacctgcg acagctccgc
     1261 ctcgtcgccg acggagatga tgagcgtatc atttgcttga taggctttcg cgccttcggc
     1321 cgcgacaaag ctttcgacca gcgtcgcccg cgcggcgctg ccgagtttga aatgcgaacg
     1381 ggtcacgatc gatgcggagg cggctgttgc gacgtgaacg atctggatcg gacgggtcag
     1441 ggagacgcca tcggccacgc tgaccacaac gccatcggtc gccaaagctg cgttgagcga
     1501 gatcatgacg tcgcttgcgt tcgcctcgag gagagcgacg ccgccgtttt ccagagcttc
     1561 gcgcagggtc cggacgctca caccggcatc ggcctcatcc gacagttcgc gcgcgaacac
     1621 gccgtcgacc agcacgagct ttgccgcccc ttcgatcgcg caggccttca gcgcggcttg
     1681 cgcacgctcg agggcggctg cgtccggggc gtccgcaagc ggcagcacct cgcgcacaag
     1741 cgcacggaga tcagtgtact tccattcctc gatgcgccga tgcggaagac ccaatgcttc
     1801 gtaagcttcg aacgcccgcc tgcgcgcgtc cgcgaccttg cccgaaccgg gcagccgatc
     1861 gcgggcagca gcgaacaagc gatcagccgc gcgtcccgtt tcggtcttta ccaaagcaac
     1921 gttcatcgtc gatccgttca agtgtcaggc cgcttcctcg aactgcgcgt agccggattc
     1981 ctccagctcg agcgccagtt ccttgccgcc actcttcacc acgcgtcccc tcaacatcac
     2041 atgcacgaag tcggggatga tgtagttcaa cagccgttga taatgggtga tcaccacgat
     2101 ggcacgatcc ggcgagcgca gtgcattgac accgtcggcg gcgatcctga gtgcatcgat
     2161 gtcgaggccg gaatccatct cgtcgaggat gcacagcgcc ggctcgaaaa gcgccatctg
     2221 caggatttcg ttccgcttct tctcgccgcc ggaaaagccg acattgacgc cgcgcttgag
     2281 catgtcttgc ggaacgttga gggattttgc gacctcacga accttcttga ggaaatccgg
     2341 cgtggagaat tcgctctcgc cgcgcgcctt gcgctgggca ttcagcgccg tgcgcaggaa
     2401 attcatggtg gcgacacccg ggatttccac gggatactgg aaggcgagaa acacgccctt
     2461 ggcggcccgc tcatccggcg ccatctccag caggtcttcg ccgttgaaca ggatctgtcc
     2521 gccagtgacc tcataacccg gcttgccggc gatcacatgc gacaaggtcg acttgccgga
     2581 gccgttcggg cccatgatcg cgtgaacctc gcccttgttc acgctgagcg aaaggccgtg
     2641 gaggatttcg cgatcctcga cacggacctg caaatctctg acttcgagca atgccattct
     2701 cgttttccca agatgtttcg aggcgctgcg gcgagcagcc ccaaggaaat tatccaaccg
     2761 atccctcaag cgagatcgaa atcagcttct gcgcttcgac cgcaaactcc atcggaagct
     2821 gctgcagcac gtccttgacg aagccgttga cgacgagacc gaccgcctct tcctgcgaca
     2881 gcccgcgctg aacgcaatag aacaggacgt cttccgagat cttcgacgtc gtcgcctcgt
     2941 gttcgaacgt cgcggatgaa ttcttcgcct cgatataggg cacggtgtgc gcgccgcatt
     3001 tgtcgccgat cagaagcgaa tcgcaggccg tgaagttgcg cgcgccgagc gctttgcgat
     3061 gggcggtgac gaggccgcga taggtgtttt gcgattttcc ggcggcgatg cccttggaga
     3121 tgatccggct cgtcgtgttc ttgccgagat gaagcatctt ggtgccggaa tcgacctgct
     3181 ggaagccgtt cgaaatcgcg atcgagtaga attctccgcg cgagttatcg ccgcgcaaga
     3241 tgcagctcgg atatttccag gtgatggccg aaccggtctc gacctgggtc caggagatct
     3301 tcgaattctt gccgcggcag tcgcc
//
LOCUS       DATLJT010000471         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2324763,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000471 DATLJT010000000
VERSION     DATLJT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324763"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VM910_10860"
     CDS             <1..323
                     /locus_tag="VM910_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63068.1"
                     /translation="RALMVRLPQSSSSYLTHGYSDTLEQQIRDTHCGQAYFANTGPFG
                     ATCGECIHLGYYQQRRNKAGDVVKATYRGGCQKFHQLTSKHGPIVPANAPACRYFERK
                     EEEK"
     gene            320..574
                     /locus_tag="VM910_10865"
     CDS             320..574
                     /locus_tag="VM910_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63069.1"
                     /translation="MSIAEQLADQILDMVEDNQIAPHTTLEAMKMVVNYTATLAVDEL
                     VEENDLAVSARIKEAVRAITNLQLEIVGVLIAHTTRETVS"
     gene            723..1133
                     /locus_tag="VM910_10870"
     CDS             723..1133
                     /locus_tag="VM910_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63070.1"
                     /translation="MVDMRKYTSGLITPDEVRDGPIEARIISVYISEKHNVPVLELET
                     GDQFFVWPQNGKKLARAYGYNSDDWLRHLIRFELGTYTDKKTGEEKETVNLTPISSRD
                     GNAGNGTPQRADPDKLPAPVERSVKDDLDDSIPF"
     gene            1595..2071
                     /locus_tag="VM910_10875"
     CDS             1595..2071
                     /locus_tag="VM910_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63071.1"
                     /translation="MTDLASIAEKLAKVVRMFGSDNPNLRAEAWKALVRTLNTFGADF
                     NDLGDRIEHAGNGALSEAEMQEIYDAGIKEGERRAAYIQRSQNQARTTHHGVFQLQFP
                     SARDMAAFCYQRIDDLNDWETEFVTNMVSWVRTRPLSLKQQAHLEKIYLKLGGEVQ"
     gene            2383..4242
                     /locus_tag="VM910_10880"
     CDS             2383..4242
                     /locus_tag="VM910_10880"
                     /inference="COORDINATES: protein motif:HMM:NF016581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HVI63072.1"
                     /translation="MRIGLPPKRAILFRTLDPFDKIMVKFTETRGEVEKIEFLCDGQQ
                     LVAEGIHPKTGKPYSWFGGTPWEIAHDDLPYISREEAQQLVDDVAEMLCRDFGYKLDP
                     KSANKTTARTNGTPPTSTNYAVVALERECAAITNALAGTRNDQINKSAFNLGQLIADG
                     LLDENMVRERLYAAAIACGYVKDDGEQAALNTINSGLRGGMAKPRQSPTVSVTSAPAP
                     GPTSTPPPSGPQPGGAQQQAPGAGTAQQPPGVGATSSTSAIDATLEVFKEWLLLKDYT
                     PVLSVLGTVAANLLPGDPVWLGLIAPPSSAKTEIVNALSGLSYVQRVGTLTPAGLLSG
                     TPRQHQAATAKGGLLRQIGQFGIIVCKDFGSILDMRPDTRNEVFSALREIFDGHWTRH
                     VGSDGGKVLTWSGKVGLLFGVTGVIDAHYAAIGAMGDRFLFNRIEPHEDQFKWALKHT
                     GAAGAQMRKELAEAITNLFATPLQQPQLLNDQETERFNYLARMAVRLRGAIARDRSTR
                     ELETVYGAEGTGRIGLALERLFMGLIALGVDRATAFAVIKTVAMDSAPPNRRRIYKHL
                     YTLKPASASTTNIAIAIRLPTKTARRALEELEAYDLIERLSQGQGKATLWCAR"
BASE COUNT         1086 a         1451 c         1345 g          877 t
ORIGIN      
        1 ggagagcgct aatggtgcgc ctgccccaat cgtcctcgtc gtatctaaca cacggttatt
       61 cggacacgct cgagcagcag attcgcgaca cgcattgcgg tcaagcgtac ttcgcaaata
      121 ccggcccctt tggcgcgacc tgcggcgagt gcattcacct tggttactac cagcagcgcc
      181 gcaacaaggc aggtgatgtc gtcaaagcca cataccgggg cggttgccag aaattccatc
      241 aactcaccag caagcacgga ccgatcgtgc ccgcgaacgc gccagcgtgc cggtacttcg
      301 aacggaagga ggaagagaaa tgagcatcgc tgaacagctg gcggaccaaa ttctcgacat
      361 ggtggaggac aaccaaattg ctccacacac aaccttggag gcaatgaaga tggtggtgaa
      421 ctacaccgca accttagctg tcgatgagct tgtcgaagag aacgatctgg ccgtcagcgc
      481 cagaattaag gaggctgtac gtgcaatcac gaaccttcag ctagagattg ttggggtact
      541 gatcgcccac acgacacgcg aaacagtgag ttgatgtttt ggcgcgcccg accttcgcgt
      601 tgcgtgtgcg tgccgagccg ggcgttgctg taattcgaag tctacgaggt tagctcaaac
      661 aaggattacg cgagttcggt ttacgctgcg tttcaatcga ggaagtaaca aaggaggatg
      721 aaatggttga catgcgtaag tacacatcgg gcctcatcac ccccgatgaa gtccgtgacg
      781 gtccgatcga ggcgcggatt atcagcgtct acatctcgga aaagcacaac gtgccggttc
      841 tggaattgga aactggcgac cagtttttcg tctggcccca gaacggaaaa aagttggcac
      901 gcgcttacgg ttacaactct gatgactggc tcagacatct gatcaggttc gagcttggaa
      961 cctacaccga caagaagact ggcgaggaaa aggaaaccgt caacctcaca ccgatctcgt
     1021 cgcgcgacgg caacgccggc aatggcacgc cgcagcgcgc cgatccggac aagctgccag
     1081 cgccggtaga gcgtagcgtc aaggatgatt tggacgacag tatcccgttt tagggcaagt
     1141 atgaaaaaga cttgcaaggc atgtggcaag accaagccgg gtgcggaatt ctatccccac
     1201 ccggcaatgg ccgacggcac acttaacttt tgtaagtcct gcatcaaaat gcgagccaag
     1261 tactacaggc tcgcaaaccc gcgcgtgcaa gagtacgacc ggcatcgctc aaaaacgcct
     1321 aagcgccgcg cacactggca agccaacgcg gtgcgctggc gacaacagaa tctaaaggat
     1381 accgcgctca caacgccgcc aataacgcgg tgcgcgaccg caagctgtcg cggtgtccct
     1441 gtcaagtttg tggcgcaact gagcatgttc ataagcatca ccgcgactat tcgcgaccgc
     1501 ttgatgtgat ctggctttgc gccaagtgtc acaaccgctt gcataccctc ttccccgaac
     1561 tgagcggaca ttcttaaaag ccggcatatg caccgtgacc gaccttgctt ccattgccga
     1621 gaagctagcg aaggtcgttc gcatgttcgg ctccgacaat ccgaatctgc gcgcggaggc
     1681 ttggaaggct ttggttcgca cactgaacac cttcggcgcc gatttcaacg atctcggcga
     1741 ccgcatcgag cacgccggca atggcgcgct gagcgaagcc gaaatgcagg agatttacga
     1801 cgccggaatc aaggagggtg agcggcgcgc cgcgtacata caacgcagcc aaaatcaggc
     1861 gcgcacaaca caccatggtg tatttcagtt gcagttcccc tctgcacgcg acatggcggc
     1921 gttttgctat caacgcatcg atgacctcaa tgactgggaa accgagttcg tcaccaatat
     1981 ggtgagctgg gtacgcacaa ggccgctgtc actgaagcag caggcgcacc tcgaaaaaat
     2041 ctacctcaag cttggcggag aagttcaatg acggcacaag aggtgctgac attacgtctc
     2101 gctctgtgtg ccaacggcta tacgccgctg ccgttgtacg gcaaagaacc gcctatctac
     2161 ggcaagaaca acaagcgcca gggactcgat aagtggcaac tgctcgataa cgttacccgt
     2221 ggacagctcg agatgtggga cctgagctgg cccgatgcct gcaataccgg aatcctgaca
     2281 cgtctgacgc caagcctcga cgccgacatc ctcaaccagg acgccgccgt tgccatcgag
     2341 gctttagtac gcgagcgttt cgaagagcgg ggttacattc tggtgcgcat cggcctgccg
     2401 cccaagcgcg cgatcctatt ccgcacgctc gaccccttcg acaagatcat ggtcaagttc
     2461 actgaaacgc gcggcgaggt cgagaagatc gagttcttgt gcgacggtca gcagctcgtt
     2521 gccgaaggca ttcatcccaa gaccggcaaa ccctatagct ggttcggtgg tacaccgtgg
     2581 gagattgcac acgacgatct accttacatc agccgggagg aggcgcagca gctcgtcgat
     2641 gatgttgctg aaatgctgtg tcgcgatttt ggctataagc tcgatccgaa gtccgccaat
     2701 aagacgacag caagaaccaa tggcacgcct ccgaccagca ccaattatgc cgtcgttgca
     2761 ctggagcggg agtgcgcggc gatcaccaat gcgttggcag gaacacgcaa tgaccagatc
     2821 aacaagagcg ccttcaatct cggccagctg atcgccgatg gcttgcttga tgaaaacatg
     2881 gtgcgcgagc ggctctacgc cgccgctatc gcctgcggct atgtcaagga cgacggcgaa
     2941 caggctgcgc tcaataccat caattccgga ttgcgcggcg gcatggcaaa gccgcggcaa
     3001 tcacccaccg tctcggtcac ctcggctccc gctcccggcc ccacttccac ccctcccccc
     3061 agcggcccgc aaccgggtgg cgcgcaacaa caggcccccg gcgcaggcac cgcgcaacag
     3121 ccccccggcg tcggtgccac gagctcgacg agcgcgatcg atgccactct cgaggtcttt
     3181 aaggaatggt tgttgttgaa ggattacacc ccggtgctca gcgtgcttgg caccgtcgcc
     3241 gctaatcttt tgcccgggga tccggtctgg ctcggactga ttgcgccacc gtcctcagca
     3301 aaaaccgaga tcgtcaatgc cctgtccgga ctgtcatacg tccagcgtgt cggcaccctg
     3361 acgccagctg gcttgctctc cggcactccg cgtcagcacc aggctgcaac cgccaagggc
     3421 ggcttgctgc gccagattgg ccagttcggc atcatcgtct gcaaagactt cggctcgatc
     3481 ctcgatatgc ggccggatac gcggaacgag gtgttctcag ccctgcgcga aatttttgat
     3541 ggtcattgga cccgacatgt cggtagcgat ggcggcaagg ttctgacctg gtccggcaag
     3601 gtcggattgc tgttcggcgt caccggtgtc atcgacgcgc attacgccgc catcggcgcc
     3661 atgggtgacc ggttcctgtt caatcgcatt gaaccgcacg aagatcagtt caaatgggca
     3721 ctcaagcaca ccggcgccgc cggcgcgcaa atgcgcaagg aattggccga ggccatcaca
     3781 aacctattcg ccacccccct gcagcagccg cagttgctga acgaccaaga gacagagcgc
     3841 ttcaactatc ttgctcgtat ggccgtgcgt ctgcgcggtg ctatcgcgcg tgatcgcagc
     3901 acccgtgagc tggaaacagt ctacggcgct gaaggaacgg gacgcatcgg cctcgccctc
     3961 gagcggctgt tcatgggcct catagcgctc ggagtagacc gggcaacggc ctttgcggtc
     4021 atcaagaccg tcgccatgga ttcagccccg cccaatcgcc ggcgcatcta caagcacctg
     4081 tacaccctca agcccgccag cgcgtcaaca acgaacatcg ccatcgcgat taggctgcca
     4141 acgaaaacgg cgcggcgcgc cctggaggaa ctcgaagcct acgacctgat cgagcgcctg
     4201 tcccagggcc agggcaaagc caccctgtgg tgcgcacgct gatcctgcag agcggccgtg
     4261 agaacagtcg gcaaattgca aaaaccccca tgaaaacggg ctttctatgc aattgcggac
     4321 gacttctccc gcaaactaca cacacacggg agagagcgta tttaattctt ccatcttcct
     4381 tatgtgtgtg tttgcgggag gggtacctaa aatgaccaag aaagccccat tttatgggca
     4441 cttctatgct ccgggcaact gttctacggg acgggtcaaa ctgaaaacca aaaatggttc
     4501 gcatccggtg cgcggggtcg tcaggatcca tggctgagcc cgccgaccga ccgactgtcg
     4561 ggtttgtcag gaaaggggat acgagccatg cggaccgtga cagcctacag gggcggtaag
     4621 agggacgacc ttcacccgct cccactcacc ccgcgcctgg gtcgaggact accggcgggt
     4681 cttcgacgcc cagttgctcg acgccggcgg tcttaatgaa ggcgcccgga gctctccacc
     4741 ctgtacggcc tcgcccccc
//
LOCUS       DATLJT010000472         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1405917,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000472 DATLJT010000000
VERSION     DATLJT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405917"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VM910_10885"
     CDS             <1..1012
                     /locus_tag="VM910_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI63073.1"
                     /translation="LLGVPMLREGVAIGVITLTRTEPRPFTDKQIELAITFADQAAIA
                     IQNVRLFEEVQARTAELSQSLAHLRAAQDRLIQTEKLASLGQLTAGIAHEIKNPLNFV
                     NNFSAVSAELVDEMDEALDDASLDDKAREKLDEIRALLKSNLDKVVQHGKRADSIVKN
                     MLLHSRSGSGDHRPVEINGIIDESLNLAYHGARAENSGFNITLERDFDPAVGIADIYP
                     QEITRVFLNLIANGFYAATRRAAEAGNGFEPTLKAATRSLGDKVEIRIRDNGAGIPEE
                     IREKIFNPFFTTKPAGEGTGLGLSMSHDIVVKQHGGTIDLATEAGAFTEFIITLPRTT
                     PA"
     gene            complement(1034..>2297)
                     /locus_tag="VM910_10890"
     CDS             complement(1034..>2297)
                     /locus_tag="VM910_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HVI63074.1"
                     /translation="STAFGNGFWSLITFTMQMAFVAIGGYVVATSAPAQRLIHWLADL
                     PKTGSGAVGLVATISILASLLNWGLSLILGGLLVLALAARRELRMDYRAAAAAAYLGL
                     GATWAMGLSSSAAQLQANAASLPKNLLTISGVIPFSETIFLWQSVLITAVLLVVSVVI
                     AIWSAPGPNTAVTAEAMGIETSRGDAINVAPPKQPGEWLEHTPILTILLALLAAGWVY
                     NEFARQDWMIAISNLNTYNFMFIMAGLLLHWRPKRFLAAVVKSVPATSGVLIQFPFYG
                     AIAAIITQAKNGAGVTVSDQITHAFVSMSTQHLYPLVIGIYSAVLGFFIPSGGGKWLL
                     EAPYVMQAANDLKVHLGWAVQVYNAAEALPNLINPFWMLPLLGVLGLRARDVVGFTFL
                     QLLLHLPIVLFLLWALAYTLEYHPPVIP"
BASE COUNT          478 a          739 c          723 g          357 t
ORIGIN      
        1 gctgctcggc gttcccatgc tgcgcgaggg tgtagcgatc ggcgtgatca ccctgacgcg
       61 gaccgagccg cggccgttca ccgacaagca gatcgagctc gccatcacct tcgccgacca
      121 ggccgccatc gcgatccaga acgtgcgtct gttcgaggag gtgcaggcgc gtaccgccga
      181 actatcgcaa tcgctcgcgc atttgcgggc tgcgcaggat cgcctgattc agaccgagaa
      241 gctcgcttcc ctcggccagc tcaccgcagg catcgcgcac gagatcaaga atcccctgaa
      301 tttcgtcaac aacttttctg ccgtatcggc cgagctcgtc gacgagatgg acgaagcgct
      361 tgatgacgcc agcctcgatg acaaggcgcg cgaaaagctc gacgaaatcc gcgccctcct
      421 gaagagcaac ctggacaagg tcgtgcagca cggcaagcgc gccgactcga tcgtgaagaa
      481 tatgctgttg cactcgcggt ccggttcggg cgaccaccgc ccggtggaga tcaacggcat
      541 catcgacgag agcctcaacc ttgcctacca cggcgcgcgc gccgagaact ccggcttcaa
      601 catcacgctg gagcgcgatt tcgatccggc ggtcggtatc gccgatatct atccgcagga
      661 gatcacccgg gtgtttttga acctgatcgc aaacgggttt tatgccgcga ccaggcgcgc
      721 ggccgaggcc ggcaacggct ttgagccgac gttgaaggcg gcgacgcgga gtctcggcga
      781 caaggtcgaa atccgcatcc gcgacaatgg cgcaggcatc ccggaggaga tcagggaaaa
      841 gatcttcaat ccgttcttca cgacgaagcc tgccggcgaa ggaaccgggc ttggcctgtc
      901 gatgagccat gacatcgtgg tgaagcagca cggcggcacg atcgacctcg cgaccgaagc
      961 cggcgcattc accgagttca tcattacgtt gccgcggacg acgcccgcgt aaacccgcag
     1021 gcctgctccc tcctcacggg atcacaggcg gatgatattc gagcgtgtag gccagcgccc
     1081 acaacaggaa caggacgatc ggcaggtgca gcagcaactg caggaacgtg aagcccacga
     1141 cgtcgcgcgc gcgcaggccg agaacgccga gcagcggcag catccagaac ggattgatga
     1201 gattgggcaa ggcttccgcg gcgttgtaca cctgcacggc ccagccgaga tgcaccttga
     1261 gatcgttggc cgcctgcatc acgtagggcg cctcgagcag ccactttccg ccgcccgagg
     1321 gaatgaagaa gcccaggacc gccgaataga tcccgatcac cagcggatag agatgctggg
     1381 tcgacatgct gacgaaggca tgggtgatct gatcggagac cgtcacgccc gcgccgttct
     1441 tcgcctgcgt gatgatcgct gcgatcgcgc cgtagaacgg aaactggatc agcacgcccg
     1501 acgtcgccgg caccgacttg acgaccgctg cgagaaatcg cttcggccgc cagtgcagga
     1561 gcaaccccgc catgatgaac atgaaattgt aggtgttcag gttggagatc gcgatcatcc
     1621 agtcctggcg ggcaaattcg ttgtacaccc agccggccgc aagcagcgcg agcaggatcg
     1681 tcaggatggg cgtatgttcg agccattccc ccggttgctt cggcggcgcc acgttgatcg
     1741 cgtctccgcg cgaggtctcg atccccatgg cctcggctgt caccgccgtg ttgggtcccg
     1801 gcgccgacca gatcgcaatc acgaccgaca cgaccagaag cacggccgtg atcagcaccg
     1861 attgccagag gaagatcgtc tcggagaacg ggatgacacc cgagatggtc agcaggttct
     1921 tgggcaggct cgccgcattg gcctggagct gcgccgccga tgacgacaat cccatggccc
     1981 aggtcgcgcc gagcccgaga taggccgccg cagccgcggc gcggtaatcc atgcgcaatt
     2041 cacgccgcgc cgccagcgca agcaccagaa gcccgccgag gatcaggctc agcccccagt
     2101 tgagcaagga agcgaggatg ctgatggtcg cgacaagacc cactgcgccg gagccggtct
     2161 tgggcagatc ggcaagccag tggatcagcc gctgcgccgg cgcggaggtg gcgacgacat
     2221 agccgccgat cgccacgaac gccatctgca tggtgaaggt gatcaggctc cagaagccgt
     2281 tgccaaacgc cgtgctg
//
LOCUS       DATLJT010000473         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1405931,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000473 DATLJT010000000
VERSION     DATLJT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405931"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VM910_10895"
     CDS             <1..249
                     /locus_tag="VM910_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63075.1"
                     /translation="GGCRLACGCWPVLPRPAEAALSREEYPPALPQLVAIDDCAMSAR
                     HVVKDAALDKRVHNVVRLAGTLDRDMPYDAIFYPRFLK"
     gene            246..1661
                     /locus_tag="VM910_10900"
     CDS             246..1661
                     /locus_tag="VM910_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI63076.1"
                     /translation="MIVDAAAPEQADLLAFGCIVTMNAENEVVPDGGIAVRDGCILAI
                     GSRTDIERRYRARRSLDRRQSILIPGLIDCHTHMSQALVRALIAHELPMIYRIYSPVT
                     QSMTPDDAHVATSLCAAQLLLSGVTTVCEGAIGRTDEHLAATVEAVSKAGVRSNIVIG
                     RADQEFQHAALYAQSRERSWLKVREGEAERELDRTEAFLARNPPGSAALITGGVCASS
                     LTAFSETYLRMAADLARRTGSKLHVHAARDREEVEFCLAVFGRRPIERLSEIGVVGRH
                     LVIAHAMLATDHELTLLGKGQSAVAHSAIECLNILNAVPNVRLMRELGITVGLGCDNA
                     VNDMFSVMRGAWILHTATRGIQGYDPEILDASAILEMATRDASAPVGLNRHIGSLEPG
                     KRADFVLLDGSGPHLLPSIDVLSELVRYACRGDVRDVVVDGRVVVDDRRLCTLDVEEV
                     QEKAETAVHRLAPIAAKGRYR"
     gene            complement(1673..2122)
                     /locus_tag="VM910_10905"
     CDS             complement(1673..2122)
                     /locus_tag="VM910_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HVI63077.1"
                     /translation="MTANLTATDIAYLHHATELAKAAEQRGNRPFGAVIVDRDGKPLA
                     EAENSVLTDDDIAAHAEMNAIRIACRAGLQRQLAGATMYASGEPCPMCTGAILRFGMR
                     RVVYGSSWDTMLPALGNQPPTVHVASRDIAALSPQEIAVIGPCESAP"
     gene            complement(2423..2881)
                     /locus_tag="VM910_10910"
     CDS             complement(2423..2881)
                     /locus_tag="VM910_10910"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63078.1"
                     /translation="MRRKIVDHDDIDPHNADAILWAMSYRCNPSMDTHIVPYRELGHG
                     PRTTNQPGLDSALLIDATMKAAMPPLALPKREYMENAKVLWEKLGLPPLKPETPWFGY
                     SLGDWTDEWDKNALDATRGKWMDRSESYRQRRRRGIIPNTPSRAAEKTED"
     gene            2849..2964
                     /locus_tag="VM910_10915"
                     /pseudo
     CDS             2849..2964
                     /locus_tag="VM910_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          520 a          894 c          923 g          627 t
ORIGIN      
        1 gggggttgcc gcctcgcctg cggctgctgg ccggtcctgc cgaggcctgc cgaggcggct
       61 ctttcccggg aagagtatcc accggcctta ccgcagctcg tagcaattga cgactgtgcg
      121 atgagcgcgc gtcacgtcgt aaaggatgcc gcactggaca agcgtgttca caacgtcgtc
      181 cgcctcgccg ggacactcga ccgcgacatg ccctatgatg ccattttcta cccgcgcttc
      241 ctaaaatgat cgtcgacgcg gcggcgccgg agcaagccga tcttcttgcc ttcggctgca
      301 ttgtcacgat gaatgccgag aacgaggttg tcccggacgg gggcatcgcc gtccgggatg
      361 ggtgcattct ggccatcggc agtcgcacgg acatcgagcg tcgctaccgg gcgcgtcgtt
      421 cgctcgatcg ccggcagtcg attctcattc cgggtctcat cgattgtcat acccacatgt
      481 cccaggcttt ggtgcgggca ctgatcgcgc acgaactgcc gatgatctac cggatctact
      541 ctccggtgac ccagagcatg acacccgatg acgcgcatgt tgcgacatcg ctgtgtgccg
      601 cgcagctctt gctctccggc gtgaccactg tttgcgaggg agccatcggc aggaccgacg
      661 agcatctcgc tgccacggtg gaggctgttt caaaagccgg cgtcagatcg aacatcgtga
      721 tcgggcgggc cgaccaggag ttccagcatg ccgcgcttta tgcgcagagc cgcgagcgct
      781 cctggctcaa ggtccgggaa ggcgaagccg agcgggaact cgaccggacg gaagctttcc
      841 tcgcccgcaa tccgcccggg agcgctgcgc tcatcaccgg cggtgtctgc gcatcgagcc
      901 tcacagcctt ctcggagacc tatttgcgca tggctgcgga cctcgctcgg cgcaccggca
      961 gcaagctcca tgttcatgcc gcgcgcgatc gtgaagaagt tgaattctgc ctggccgtgt
     1021 tcggacggcg gccgatcgag cgactgtcgg agatcggtgt cgtcggcagg catctcgtca
     1081 tcgcgcatgc catgttggca accgaccatg aattgactct cctcggcaaa gggcaatcgg
     1141 ctgtcgcgca ttctgccatc gagtgcctga atattctcaa cgctgtgcca aacgtgcgcc
     1201 tgatgcgcga gctcggaatc acggttggtc tcggatgcga caatgccgtc aatgacatgt
     1261 tctccgtcat gcgcggagcg tggattctgc acacggcgac gcggggtatt cagggatatg
     1321 accccgaaat cctcgatgcc agcgcgatcc ttgaaatggc gacaagggat gccagtgcgc
     1381 cggttgggct gaatcgacac attggctctc ttgagccagg gaagcgagcc gattttgttt
     1441 tgctggatgg ctcggggccg catttgcttc ccagcatcga cgttttatcg gagctggtcc
     1501 gatatgcctg tcggggcgac gtgcgagatg tcgtcgtgga tggccgggtg gtggtcgacg
     1561 accggcgctt gtgcacattg gatgtcgagg aagttcagga gaaggctgaa acggcagttc
     1621 atcggttggc gccgatagct gcaaagggcc gatatcggtg accggtctcg atctacggcg
     1681 cggactcgca gggtccgatc acggcgatct cctgcggaga gagcgcggcg atgtccctgc
     1741 tcgcgacgtg aacggtcggc ggctgatttc ccaaggccgg caacatggtg tcccagctgc
     1801 tgccgtatac aacgcggcgc attccaaacc ggaggatggc gcccgtgcac atcggacaag
     1861 gttctccgct cgcgtacatg gtggcaccgg caagctgtcg ttgcagccct gcccggcagg
     1921 cgattcggat cgcgttcatc tcagcatgtg cggcgatgtc atcgtcagtg agcaccgagt
     1981 tttcggcctc cgccagcggt ttgccatcgc gatctacaat gaccgcacca aatggccgat
     2041 tgccgcgttg ctcagccgcc ttcgcgagct ctgtggcatg gtgtagatac gcaatatcgg
     2101 tggccgtcag atttgctgtc acctttcgct cctttgtcga tgccgtgtag atggcgctcc
     2161 tctgttgtcc accggttgcc gcttcggcga actaaatccg agcgcatcgc accctacaca
     2221 ccggatcgtc tatcagcagc cgatcaggga gtggcaaggg taaacggttc taccgcggtt
     2281 ggtgcgggcg aagtaaccaa cgtggcacag gggcgcgtga ggtaattttg cctcggcggc
     2341 cacgccgcca tgcgccgagc aggaactcgc gccgtgtcac tcgatcacct cgtcggcgcg
     2401 ggcgagcaga gatagtttcc agctagtcct ccgttttttc cgctgcacga cttggggtat
     2461 tcgggatgat gcctcgtcgc cgacgctggc ggtacgactc gcttcgatcc atccatttgc
     2521 cacgagtcgc atcgagcgcg ttcttatccc attcgtccgt ccagtcgccg agcgaataac
     2581 caaaccatgg agtttccggc ttgaggggtg gcaagcctag tttttcccac agcactttgg
     2641 cgttttccat gtattcgcgc ttgggcaatg caagcggtgg cattgcagcc ttcatggtcg
     2701 catcgatcag gagcgccgaa tctagcccgg gttggttggt ggtgcgtggc ccgtgaccca
     2761 gctcacgata cggtacgata tgtgtgtcca tggatggatt gcagcggtag gacatggccc
     2821 agagtatcgc gtctgcattg tgcgggtcga tatcgtcatg atcgacaatc ttcctgcgca
     2881 caaagctgct ggcatacgcg aagcgattga agcgcgcggc gctacgcttc gctatctgcc
     2941 ccagtactcg cccgacctca atcc
//
LOCUS       DATLJT010000474         4947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3474199,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000474 DATLJT010000000
VERSION     DATLJT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3474199"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..414)
                     /locus_tag="VM910_10920"
     CDS             complement(262..414)
                     /locus_tag="VM910_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63079.1"
                     /translation="MRASIGLPAGEIFVKYPGGDGSEPDVAWQCTFADVAARDADLAV
                     RAASGN"
     gene            complement(602..1585)
                     /locus_tag="VM910_10925"
     CDS             complement(602..1585)
                     /locus_tag="VM910_10925"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVI63080.1"
                     /translation="MALDTFISYSSPDKTAADAACAVLERAGIRCWIAPRDIRPGQEY
                     GHAIIEAIDQCRVMVLIFSANANDSRQIHREIERAVSKGVPIIPLRIEEVVPTRSMEY
                     FLGAIHWLDALTPPLEKHLQQLAEAVKALLQIDVAGSTAPLRDNSSQASTTVRPAEPA
                     MQGGRRAAWSTAAGRLLILLLGVAAACIALLIGGVWHYLMREPAAVTTSAQAVAASLS
                     RTVQSGIMTEVAEHVGFDKSCVPQHVVVKITRPPTNGTATTAEENKSLPARTELGGTQ
                     PCAGKSAPTAVVYYQSKPDFRGTDQFRYQRTNQDNPNDRLNGEILITLTVK"
     gene            complement(1770..2195)
                     /locus_tag="VM910_10930"
                     /pseudo
     CDS             complement(1770..2195)
                     /locus_tag="VM910_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            2427..3578
                     /locus_tag="VM910_10935"
     CDS             2427..3578
                     /locus_tag="VM910_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63081.1"
                     /translation="MAPIIWLGYRALQRPPGTWNVGGNAKRWIRKIAYADDLERANGK
                     TILDFWQGQEAAKKVARGEDGTAGDRPHTVGEALDSYARDLRAKGGDTYNASRARQHL
                     KGNAMLTTPVALLKEAELKGWRDALIAKELAPATVNRTMVPLHAALEAAADGDQRIIR
                     RPWRTALRRVSDANNVRRIVKEPDDIRKLVAACYEADHQLGNLCEVLATCGMRLSQAS
                     RLTVADLQADSNPRLLVPKSAKGNLRKRSERIPVPIPPALAALLQQEAAGRSPNEPLL
                     RQAAGTPWQHRSDADHRQLFRAAVESAGFDPGEVTSYCFRHSSIVRQLLANVPIRIVA
                     ATHDTSVGQIERCYSRFVTDHSDGLTRAALLDLSASASDSKVVPLTREP"
     gene            4049..4174
                     /locus_tag="VM910_10940"
     CDS             4049..4174
                     /locus_tag="VM910_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63082.1"
                     /translation="MIRFLADNGVRLDSVKYEENYSAIDYDVIEPVEAELGRLSN"
     gene            4364..4744
                     /locus_tag="VM910_10945"
     CDS             4364..4744
                     /locus_tag="VM910_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63083.1"
                     /translation="MSAERPSDIVTIPRAEHERLLAIATGQGLSSETVIIPRAEYEHL
                     LAVAAGDGPADTIKTFCDSERISQAFFHVMQDEGWAPDITYHGEAARILPAAKRKWRS
                     ERARASAAGLRRDLLRGPAPPEAA"
BASE COUNT          988 a         1557 c         1481 g          921 t
ORIGIN      
        1 gcaacaggat atgccagccg atcacccagg cgaactgcag gcgcgacagc agaaccggat
       61 cgagatccat ggtcgccccc ggcgacaaga cggacaaacg ctccgcatcc gtgcgcttga
      121 ggttaccccg atgctgccac caaggccagt cctcgaatcc ggaacggctc tctctagcta
      181 gagccgcgca cgatcttcga atcggcaatc aaagcgacgc gatcgcaaag acttgccgct
      241 cgaaacgtgc atagagttgc ctcaattgcc gcttgcggcg cgcaccgcga ggtcggcgtc
      301 gcgtgcggcg acgtcggcga aggtacattg ccaagcgaca tccggctcgc tgccgtcacc
      361 gccgggatat ttgacgaata tttcaccggc tggaagcccg atcgaggcgc gcacggcgga
      421 agccttgcgt cgttatcgag gaccgttttg gcctcattta ttgcagtgca tgagtccggt
      481 tctggcacca agcgggttta gcgaggcgtc tgcctattag gctgcttttg gtgaaaagcg
      541 gcagccggtg aagccgttgc ctgcattggt ccgacgggcg ccgcgaacga tagggtaggg
      601 gttacttcac cgtgagggtg atgagaattt cgccgttcag ccggtcgttc ggattgtcct
      661 gattcgtgcg ctgatacctg aattgatcgg tgcctctaaa atccggcttg gactgataat
      721 agacgacggc tgtcggcgca gactttccag cacatggttg ggtcccgcca agctcggtcc
      781 tcgccggcaa gcttttgttt tcttctgctg tcgtcgcggt tccgtttgtg ggtggcctgg
      841 taatcttcac cacgacgtgc tgcggcacgc aggatttgtc gaagccgacg tgttctgcaa
      901 cctctgtcat aatgcccgac tggaccgttc gcgaaaggga tgccgcaact gcctgagctg
      961 acgtagtcac ggcagcgggt tcacgcatca gataatgcca aacgcctccg atcagcaggg
     1021 ctatacaggc agcggcgact cctaagagga gaatgagcag gcgccctgct gcggtcgacc
     1081 aggcagccct tctaccgcct tgcatcgcag gttcggcggg tcgaactgtg gttgaagctt
     1141 ggctgctatt gtcacgaagc ggcgccgtcg atcccgcgac gtcgatctgc aacaatgctt
     1201 ttacagcctc cgcaagctgc tgcagatgct tttcaagagg cggcgtcagc gcgtcgagcc
     1261 aatgaatggc gccgaggaaa tattccatgg accttgttgg gacgacctct tcgatccgca
     1321 gagggataat cggaacacct ttgcttacag ctcgctcaat ctcgcgatgg atctgccgtg
     1381 aatcgttggc gttggcggag aaaatcagca ccatcacgcg acattgatcg atcgcctcga
     1441 tgatagcgtg tccatactct tggccgggcc tgatgtcgcg cggcgcaatc caacaccgga
     1501 ttcccgcccg ctccagcacg gcgcaagcgg catctgccgc cgttttatcc gggcttgagt
     1561 agctaatgaa cgtgtctaaa gccattgcgc tgtcgagacg cccccgcacg agcacggaca
     1621 acgcgtgcgg ccacggtttc agattggatt atacttggcg gctggactcg atgccagccg
     1681 accagcgata gggatgcggc aacaagggca aattcagccc caagaagaag caatagcccg
     1741 cctcggcata ttcggccgcc ctcgactgat cagtccggct ttgcgttcga gaatttcacc
     1801 accttcgccc acttctcgat ttcgtccgcg atgaacgttc cgaattgagc cggcgtcatc
     1861 ggcatcggct cgccgccaag ctccgcaagc cgcgcttcga gcttcggatc ggcgagtacc
     1921 gcgttcaccg ccttgttcag cgtgtcgacg atctcgggcg gcgtaccctt gggcgccacg
     1981 atgccgtacc acaccacggc ttcgtagccc gggactgttt cgccgatcgt cggcacgtcc
     2041 gggatcgcgg cggcgcgctt gcccgccgtc acgcccagcg cgcgcagcgt gccggccttg
     2101 atgtgctcga ccgagccggg caggttgtcg aacatgacct ggacgcggtt gctgagcagg
     2161 tcctgcatcg cagccgccgt gccgcggtaa ggcaattctt gtcgcatagc gctccatagc
     2221 gctcttttga cccattggta agaggctgaa atccctagtt atagattagc atagagtgct
     2281 atgaggcaaa tacgcctaat gacactctat cccactcttt aggacatacg cggggacacg
     2341 ccatggccaa agtccgcgac gcgcgactcg aaacccgtac ggcacgccgt cgactcaagc
     2401 cgcggcgcaa gccgtactgg gtccgagtgg cgccgatcat ctggttaggt taccgcgccc
     2461 tgcaacggcc gcccggcacc tggaacgtcg gcggcaacgc gaagcgctgg atccgcaaga
     2521 tcgcctacgc cgacgatctg gagcgcgcga acggcaagac catcctggac ttctggcagg
     2581 ggcaggaggc ggccaaaaag gtggcgcgcg gagaggacgg caccgccggc gatcgcccgc
     2641 acacggtggg cgaagcgctc gatagctatg cgcgggatct tagagctaag ggtggcgaca
     2701 cttacaacgc gagccgagcg cgccagcacc tcaagggtaa cgcaatgctc accacccctg
     2761 ttgcgttgct caaggaggct gagctgaagg ggtggcgcga cgcgctgatc gccaaggagc
     2821 tcgcgccagc gaccgtgaac cgcaccatgg tccccttgca tgcagcgttg gaggcagctg
     2881 ctgatggcga ccagcgcatt atccgacgcc cctggcggac cgccttgcgc cgggtctccg
     2941 acgccaacaa cgtgcggcgc atcgttaagg agcccgacga catcaggaag ctggtcgccg
     3001 cctgctacga ggcggatcac cagcttggga acctctgtga ggtgctcgcc acgtgcggaa
     3061 tgcggctttc gcaagccagc cggctcaccg tggccgacct acaagccgac tccaatccgc
     3121 gactgctggt gccgaaaagc gccaaaggca atctgcgcaa gcgcagcgag cggattccag
     3181 tcccgatacc gcccgcacta gccgcgctgc tgcaacagga agccgctggt cgatctccga
     3241 atgagccgct gctgcgtcag gctgctggca ctccctggca gcaccgcagc gacgccgacc
     3301 accgccagct ttttcgcgcc gccgtcgaga gcgcgggctt cgatccaggc gaagtgacaa
     3361 gctattgctt ccggcacagc tcaatcgtgc gccagctact cgccaacgtg cccatcagaa
     3421 tcgtcgcggc cacgcacgat acgagcgtgg gccagattga acgttgttat tctcgcttcg
     3481 taaccgacca cagcgatggg ctaacccgcg ccgcgttgct cgacctgtcc gcgtccgcgt
     3541 ccgattcaaa ggtggtgccg ctgacgcggg agccttaagt taggagcccg gccagcacgc
     3601 tcaatgctgg accgggccag atattgtcca cccaaccgcc aagctgagag gacgacgtta
     3661 catgtatagg cactcgcagg ttttttcgca aattggtcta gcatgtgaga ttcctcccac
     3721 cctccaacac tttcttgacg cactccatca tcacccaccc ccacccgacg gaatccgtgg
     3781 ctttctgctt ccgagtgacc accctctgct ccggtggatg gcagcccaag ccgcacaatt
     3841 gcagcagcag cagcagcagg atgccacgac gcgccgcccg cagcttcggt cgcggtcact
     3901 tccaccgacg gggagtgcgg acaacgcgcg cgcggcaaac aggggcggcc gcccgcgctt
     3961 aatccccgcc aagtacatcg gaccagcaag agaagatctg aaggcctcag gcgtccaact
     4021 acggccgcgc aaaggtccga gagcccgagt gattcgattc cttgcggaca acggcgttag
     4081 gctcgattcg gtaaaatatg aggagaacta cagcgcgatt gactatgatg tgatcgagcc
     4141 ggtggaagct gagcttggac gcctttccaa ttagggtttt tcgcgaaatg cgaaaaaccc
     4201 cctcttagtt tctcgcgcga aaaacccctt gtggtttctc gtgcgagaaa ccattcctcg
     4261 aacaaaatct cgcgaaaaac ctaggttttt cgcaacgtag aattcgatta aaggttggcc
     4321 gaggcgcata accatctcta tggcactcgt catggaggtt ggattgtccg ccgagcgtcc
     4381 ctcagacatt gtcacgattc cgcgcgccga gcacgaacgg ctgctggcga tcgcgactgg
     4441 tcagggactg tcctccgaga cggtcatcat cccgcgcgcc gaatacgagc atttgctcgc
     4501 tgtcgccgca ggtgacgggc cagccgacac gattaagacg ttttgcgaca gcgagcggat
     4561 ctcccaagcg ttttttcatg tgatgcaaga cgaggggtgg gcgccggata tcacatatca
     4621 cggcgaagca gcgcggatcc tgcccgccgc caagcggaaa tggcgcagtg agcgagcccg
     4681 cgcatccgct gccggccttc gccgcgacct cctgcgcgga ccggcaccgc cggaggccgc
     4741 gtgatttgaa tcggcgcgcg ccgcttggcc gccagcggtg catggttggg ggcggcatgt
     4801 tggggactgc gatcctcaac gcaaaccggc cagcgagacc tcgcggtcgc actggccggg
     4861 aaactgaaac gtccaacagg cccgctacgg ccagaaagga caggtactcg atggccagcc
     4921 cttataagga attctctcgc cccgtca
//
LOCUS       DATLJT010000475         5254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1635977,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000475 DATLJT010000000
VERSION     DATLJT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635977"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VM910_10950"
     CDS             complement(<1..649)
                     /locus_tag="VM910_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HVI63084.1"
                     /translation="MPIDSSKFFDRIRIKPNKLSAKQEAQAREEAVMCEWPGCQSRGP
                     HRAPKGRNQAREYFHFCLNHVREYNQSYNFFSGMNAEAVARYQKDALTGHRPTWRMGA
                     NGSGGKGKGASAPDFDGAADPFSVFSELNGRGRWRPGPGSEARAETRKVFNAERKALQ
                     VMGLGPDATLKEVKVKYKALVKQHHPDANGGDRSTEDRLIEIIKAYNYLKTVVRAS"
     gene            760..1029
                     /locus_tag="VM910_10955"
     CDS             760..1029
                     /locus_tag="VM910_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HVI63085.1"
                     /translation="MATRDIIINKLREAFFPESLEVTDESHLHEGHAGHRPGGETHFR
                     VYIVSPAFKGKSRVERHRMINTTLSSELAGSVHALAIHAQAPGEG"
     gene            complement(1034..2341)
                     /locus_tag="VM910_10960"
     CDS             complement(1034..2341)
                     /locus_tag="VM910_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HVI63086.1"
                     /translation="MDWLTISIVVALLCASAFFAASETALIGASRASMLRLSKQGNGE
                     ARVVYGLIGMRQRLIGALLLGNNIANIGASALATGIFTSWFGEVGVLYATAVMTITVV
                     IFAEVLPKTIAINAPDRVSLAVARPMRLTMYVLGPLLTVIEAVVRLMVKLLGIKIGAN
                     QPLLSPTERLRGAVDLLHSEGKVEKQDRDMLGGLLDLRELQVSDVMIHRTEVVMVNAD
                     LPPEDLVGEVLATEFTRIPLWRDKPENIIGVLHAKDLLRAIRASEGDMSRIDVSSLAL
                     PPWFVPEMRPLSEQLKAFRRRKTHFAMVVDEYGEFEGIVTLEDILEEIVGDISDEQDV
                     VVAGVRAQPDGSVVVDGSVPIRDLNRAMDWDLPDEEATTVAGLVIHEARSIPERGQSF
                     TFHGFRFRVLRRERNRITALRIQPVPREAGIEEKKPRRAGSSF"
     gene            complement(2352..3500)
                     /gene="aroB"
                     /locus_tag="VM910_10965"
     CDS             complement(2352..3500)
                     /gene="aroB"
                     /locus_tag="VM910_10965"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HVI63087.1"
                     /translation="MTAPLKQSEVTIVDVALGERAYDIVIGRDVLASLGARIAALRPG
                     VRAAVVTDRTVAKHWLEKTEASLLQAGIATSRIIVDEGEGSKTYAGLKQVSEALIAEK
                     IERNDLVIALGGGVVGDLAGFAAAILRRGVDFVQVPTSLLAQVDSSVGGKTGINSPQG
                     KNLLGAFHQPVLVIADTAVLDTLSPRQFRAGYAEVAKYGVLGDEAFFAWLEKNHADVF
                     RGGAAREHAIATSCRAKAAIVARDERETGDRALLNLGHTFGHALETATGFSDRLFHGE
                     GVAIGMVLAAEFSATLGMIAQTDAERVAHHLAEVGLPTHLQDIAGFAQEGLADADALM
                     TLMAQDKKVRRGRLTFILLEAVGRAVIARDVEPVTVRDFLKARLAGKN"
     gene            complement(3497..4120)
                     /locus_tag="VM910_10970"
     CDS             complement(3497..4120)
                     /locus_tag="VM910_10970"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HVI63088.1"
                     /translation="MSESTSHAAVGTSSEAGILAALGPRLIVLVGMMGAGKSTIGRRL
                     AARLRLPFIDADSEIEAAASMSIPEIFEIHGEPHFRDGEARVIARLLESGPAVLATGG
                     GSFMREETRRRVRDKAVSIWLKADADIIMRRVKRRADRPLLRTADPAATVGRLIAERE
                     PVYQHADLTICSRDVPHDKIVEECIEALHALLGGRAKPVTDPVGAAR"
     gene            4263..4409
                     /locus_tag="VM910_10975"
     CDS             4263..4409
                     /locus_tag="VM910_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVI63089.1"
                     /translation="MPSLFRFLTVLAVIAGIVYGAIFALANFVNPRPREMSVTIPPDK
                     FLKK"
     gene            4437..>5254
                     /locus_tag="VM910_10980"
     CDS             4437..>5254
                     /locus_tag="VM910_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63090.1"
                     /translation="MRAKASDAKLIDLFLDMLAAEQGAGENTLAAYRRDLTDLSDYVA
                     RAGAGFVGVKTEGLRGYLADLDTRGFKSSSVARRLSAMRHLFRFLLNERIRSDDPAAI
                     LSGPKRGRGLPKVLSIADVDRMLTRARELAQAPGSSGSQRLRAMRLYCLLEVLYATGL
                     RVSELVALPRSAARHDARMIVVRGKGNKERLVPLNDASRAAMRDYLATMETLTQEKKQ
                     NGTSSKWLFPSFGESGHLTRQHFARDLKDLASAAGLAPRLVSPHVLRHAFASHLL"
BASE COUNT          944 a         1727 c         1640 g          943 t
ORIGIN      
        1 agctcgcgcg caccaccgtc ttcagataat tatacgcctt gatgatctcg atcagacgat
       61 cttcggtcga gcggtcgccg ccattggcgt cgggatgatg ctgcttcacc agcgccttgt
      121 actttacctt gacctctttc agcgtcgcgt ccggaccgag ccccatcacc tgcagtgctt
      181 tccgctcggc gttgaagacc ttccgcgtct cggcccttgc ttcgctgccc ggtcccgggc
      241 gccaccggcc gcggccgttg agctcggaga acacgctgaa cggatcggcg gctccgtcga
      301 aatcgggggc ggatgcaccc ttgcccttgc cgccactgcc attggcgccc atccgccagg
      361 tcggacgatg gccggtcagc gcatcctttt gatagcgtgc gaccgcctcg gcattcatgc
      421 cggaaaagaa attgtaggac tggttgtatt cacgaacgtg attgagacag aaatggaagt
      481 attcccgcgc ctgattgcga cccttgggcg cacggtgcgg gccccggctt tggcagccgg
      541 gccactcgca catgacggct tcctcgcgcg cctgcgcctc ctgcttcgcg ctcagcttgt
      601 tcggcttgat gcgaatgcgg tcgaagaatt tcgatgaatc gatcggcatg gcaaactttg
      661 actacgcaac gcgaaaagct tcaagtcttg acattgcgaa acccttcaac tccgagaagc
      721 gccattgacg gcaacgcgcc ggggacctat gtcgccgcca tggccacccg agatattatc
      781 atcaacaagt tgcgtgaagc tttctttccc gagagcctcg aggtgacgga cgaatcacat
      841 ctgcatgagg gccatgccgg ccacaggccc ggcggggaga cgcacttcag ggtgtatatt
      901 gtatcgccag ccttcaaagg gaagagccgg gtcgaacgtc accgcatgat aaatacgacg
      961 ctttcgagcg aactcgccgg ttccgtgcac gcactcgcca tccacgcgca ggcgcccggc
     1021 gaaggctgat cggctagaag gaggagcccg cccgcctcgg ctttttttcc tcgatcccgg
     1081 cctcacgcgg aactggctgg atcctgagcg cagtgatgcg gttgcgctcg cggcgcagca
     1141 cgcggaagcg gaagccatgg aaggtgaagc tctggccacg ctcggggatc gaacgagcct
     1201 cgtggatcac gagtcccgcg accgtcgtcg cctcctcgtc gggcaggtcc caatccatcg
     1261 cacggttgag atcacggatc ggcaccgagc cgtcgaccac gaccgagccg tcgggctggg
     1321 cgcggacgcc tgccaccacg acgtcctgct cgtcggaaat atcgccgaca atctcttcga
     1381 gaatgtcctc gagggtcacg atgccttcga actcgccata ctcgtctacc accatggcaa
     1441 aatgcgtctt gcgccgacgg aacgccttga gctgctcgga aagtggacgc atctccggca
     1501 cgaaccaggg cggcagcgcg aggctggaga cgtcgatgcg cgacatgtcg ccctcggaag
     1561 cgcgaatggc gcgcagcaaa tccttggcgt gcagcacgcc gatgatgttt tccggcttgt
     1621 cgcgccagag cgggatacgc gtgaattcgg tcgccagcac ctcgccgacc aggtcctcgg
     1681 gaggcagatc ggcattcacc atgaccactt cggtgcggtg aatcatcaca tccgacacct
     1741 gcaactcgcg caggtccagc agcccgccga gcatatcgcg gtcctgtttc tccaccttgc
     1801 cttcgctgtg gagcagatcg accgcaccgc gcagacgctc ggtcggcgac agaagcggct
     1861 ggttggcgcc gatcttgatt ccgagcagct tcaccatcag acggaccact gcctcgatca
     1921 ccgtgagcag cggccccagc acatacatcg tcagtctcat cggccgggcg accgccagcg
     1981 agacgcggtc gggggcgttg atcgcgatgg tcttgggcag cacctcggcg aagatcacca
     2041 ccgtgatagt catgacggcg gtggcgtaga gcacgccgac ttcgccaaac catgaggtga
     2101 agatgccggt ggcgagcgcg gaggcaccga tattggcgat gttgttgccg agcaggagcg
     2161 cgccgatcag gcgttggcgc atcccgatca ggccatacac gacgcgcgcc tcgccattac
     2221 cctgcttcga cagccgaagc atgctcgcgc gcgaagcgcc gatcagcgcc gtctcgcttg
     2281 ccgcgaagaa ggccgatgcg cagagcagcg ccaccacaat ggaaattgtc agccagtcca
     2341 tgacgcggtc gtcagttctt gccagccagc ctcgccttga ggaaatctcg cacggttacg
     2401 ggctcgacat cccttgcgat caccgcgcgg ccgacagcct cgagcaggat gaacgtcaag
     2461 cggccgcgcc ggaccttctt gtcctgcgcc atcaaggtca tcagcgcatc cgcatccgcc
     2521 agcccctcct gtgcgaaacc tgctatgtcc tgcagatggg tcggcagccc gacctcagca
     2581 agatggtggg cgacgcgttc cgcatccgtt tgcgcgatca taccgagggt tgcggaaaat
     2641 tccgccgcga gcaccatgcc gatggcaact ccctcgccat ggaacaggcg gtcggaaaag
     2701 ccggttgcgg tttccagcgc gtgaccgaaa gtgtgaccga gattgagcag cgcgcgatcg
     2761 ccggtctcgc gctcgtcgcg cgcgacgatc gcggccttgg cgcggcagga ggtggcgatc
     2821 gcgtgctcgc gtgccgcgcc gcccctgaag acatcagcgt gatttttctc cagccaggcg
     2881 aaaaatgctt cgtctccgag cacgccatat ttggcgacct cggcatatcc cgcgcgaaac
     2941 tggcgtggcg acagcgtatc gagcaccgcg gtatcggcga tgacaagcac cggttggtgg
     3001 aaggcgccaa gcaggttctt gccctgcggc gagttgatcc cggtcttgcc gccgacggag
     3061 gagtcgactt gcgccagaag cgaggtcggc acctgcacga aatcgacgcc gcgccgcagg
     3121 atcgcagcgg caaagcctgc gagatcaccc acaacgccac cacccagtgc aatgacgaga
     3181 tcgttgcgct cgattttttc ggcgatcagc gcttccgaaa cctgtttgag cccggcatag
     3241 gtcttggagc cctcaccttc gtcgacgatg atgcgggacg ttgcgatgcc agcctgtagc
     3301 agcgaagcct cggtcttctc cagccagtgc tttgcgacgg tgcggtcggt gacgacggcg
     3361 gcgcggacac cgggccgcaa cgctgcgatg cgtgcgccca gcgaggccag cacgtcgcgg
     3421 ccgatcacga tgtcataggc acgctcgccg agcgcgacgt cgacgattgt cacctccgac
     3481 tgtttcagcg gtgcggtcat cgtgcggcac ccaccggatc ggtcaccggc tttgcgcgac
     3541 cgccaagcag cgcgtgcagg gcttcgatac actcctcgac gatcttgtcg tgcggcacgt
     3601 cgcgcgagca aatggtcaga tcggcgtgct gatagaccgg ctcacgttcg gcgatcaggc
     3661 gaccgacagt tgccgcggga tccgctgtcc gcagcagagg tcggtcggcc cggcgcttga
     3721 cccgacgcat gatgatgtcg gcgtcggctt tgagccagat cgagaccgcc ttgtcgcgga
     3781 cgcggcgacg ggtctcctcc cgcatgaagg aaccgccgcc tgtggcaagc accgccgggc
     3841 cgctttccag gagccgtgca atcaccctcg cctcgccgtc cctgaaatgc ggctcgccat
     3901 ggatctcgaa gatctccgga atcgacatgc tggccgccgc ctcgatctcg ctgtcggcgt
     3961 caatgaaggg cagccgcagc cgcgcggcga gccgccgacc gatggtcgat tttcccgcgc
     4021 ccatcatgcc gacgagcacg atcagccgcg gcccgagggc tgcaagaatt ccggcctcgg
     4081 aagaggtgcc gacggcggcg tgcgacgtgc tctcggacat gttcccggtt caaaaggggc
     4141 ggcctctgcc gctttcttgc cacctatact acccccgccg atgcccgtac cagagactct
     4201 tgcaagggcg ggacgaacat gttggatgtc cttggttaat ttacgtatct caaggcccag
     4261 agatgcccag cctgttccgc tttctgacgg tcctcgccgt gatcgccggc atcgtctatg
     4321 gcgcaatttt cgcgctggcg aatttcgtca atccgaggcc gcgggaaatg tcggtcacca
     4381 ttccgccgga taagtttctc aagaagtagg cggctgggtc gggggatatc agggggatgc
     4441 gcgcgaaagc ctcggatgcg aagctgatcg acctgttcct cgacatgctc gcagcggagc
     4501 agggcgcagg cgaaaacacg ctcgccgcct atcggcgcga tctcaccgat ctctccgatt
     4561 atgtcgcccg tgccggggca ggttttgtcg gcgtcaagac cgagggtctg cgcggctatc
     4621 ttgccgatct cgatacgcgc ggcttcaaat catccagcgt cgcgcgacgg ctatcggcga
     4681 tgcggcatct gttccgcttc ctgctcaacg agcggatccg cagcgacgac ccggcggcga
     4741 tcctgtccgg gccgaaacgc ggccgcggcc tgcccaaggt gctgtcgatc gccgacgtcg
     4801 atcgcatgct gacccgcgcc agggaactcg cgcaggcgcc cggctcatcg ggctcgcagc
     4861 ggctgcgcgc gatgcggctc tattgcctgc tcgaagtgct ctatgccacg ggcctgcgcg
     4921 tctccgaact ggtggcgctg ccgcgctcgg cggcgcgaca cgacgcgcgc atgatcgtgg
     4981 tgcgcggcaa gggcaacaag gagcgcctgg tgccgctgaa cgatgcctcg cgggcggcca
     5041 tgcgcgacta tctcgcgacg atggaaacgc tgacgcagga gaaaaagcag aacggcacgt
     5101 cgtcgaaatg gctgtttccc tctttcggcg aaagcggaca cctgacgcgg cagcattttg
     5161 cgcgcgacct caaggacctg gcgtccgccg cagggctcgc gccgcgactg gtgtcgccgc
     5221 atgtgctgcg ccacgccttc gcaagccacc tcct
//
LOCUS       DATLJT010000476         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_487353,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000476 DATLJT010000000
VERSION     DATLJT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487353"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VM910_10985"
     CDS             complement(<1..761)
                     /locus_tag="VM910_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63091.1"
                     /translation="MDRRQFVLGAASLPLLPGTLRAQTKPLKIGVMNDLSSVYADFQG
                     FGSKVAADLAVADYAKSLGVPVEVIIADHQNKPDIGSVIARKWFDTEGVDVIMDLPNS
                     GVALAVLAVANEKNKAVIGSGAGSAVLTGPQCSKNFVHWTYDTYALGYGLGQAITKEG
                     GKTWFILTADYTFGKDLEQNCSDAVTANGGKVLGVVRHPINTADFSSFLLQAQSSGAD
                     VVAFANAGGDTSQSLKQAAEFGLGQQQRLAALVFDL"
     gene            complement(788..>1514)
                     /locus_tag="VM910_10990"
     CDS             complement(788..>1514)
                     /locus_tag="VM910_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI63092.1"
                     /translation="PETAAHYREQNVAAVIFPVDAERETGYRRYKNEEVAEIAAENSD
                     ILIPFASIDPHKGKFGAREARRLVNEFGVQGFKFHPTMQGFYPNDRLAYPLYEAIAET
                     GKPALFHTGQTGVGSGMRGGNGMRLKYSNPMYIDDVAVDFPDMPIIMAHPSFPWQEEA
                     LSVANHKPNVYIDLSGWSPKYFPPILVQYSNTLLRKKVLFGSDWPMISPERWLADFEK
                     IEIKNEVRPLILKENAARLLKLV"
BASE COUNT          309 a          448 c          437 g          320 t
ORIGIN      
        1 aggtcgaaca ctagagcggc gagccgctgt tgctgtccga gaccgaattc ggcggcctgc
       61 ttcagcgact gactggtgtc gccgcccgcg ttggcgaagg cgacgacgtc cgcgcctgaa
      121 gattgcgcct gcagcaggaa ggacgagaaa tccgccgtat tgatcggatg ccgcacgacg
      181 ccaagcacct tgccgccgtt tgccgtcacc gcatcggagc agttctgctc gagatcctta
      241 ccgaacgtat agtcggcggt tagaatgaac caggtcttgc cgccttcctt ggtgatcgct
      301 tgaccgaggc cgtagcccag cgcataagtg tcataagtcc agtgcacgaa gttcttcgag
      361 cattgcggac ccgtcagtac cgccgagccc gcgccggatc cgatcaccgc cttgttcttc
      421 tcgttggcga ccgcgaggac tgcgagcgcc acccccgagt tcggcaggtc catgatgacg
      481 tcgacgccct cggtgtcgaa ccactttcgc gcaataaccg aaccgatgtc aggcttgttc
      541 tggtgatcgg cgatgatcac ctcgaccggc acgccaagcg atttcgcgta gtcggcgacg
      601 gcaaggtcgg cggcgacttt cgagccgaac ccctggaaat ctgcgtagac gctcgaaaga
      661 tcgttcatta cgccgatctt cagcggtttt gtctgcgcgc gaagcgtccc aggaagcaac
      721 ggaagagaag cggcgccgag cacgaattgc ctgcggtcca tggatgcgtc tcctgaaaag
      781 atgatggtca cacaagcttg agaagccgag ctgcattctc cttgaggatg agcgggcgca
      841 cctcgttctt aatctcgatt ttctcgaagt ccgcgagcca acgctccggg ctaatcatcg
      901 gccagtcgga tccgaacaac actttcttcc tgaggagtgt gttggagtat tgaaccagaa
      961 tcggcgggaa gtacttagga gaccagcctg acaggtctat gtaaacgttc ggcttgtggt
     1021 tagccaccga cagcgcctcc tcctgccatg gaaacgacgg atgcgccatg atgatcggca
     1081 tgtcaggaaa atccacggcg acatcgtcga tatacatcgg gttcgaatat ttcagccgca
     1141 tgccgttgcc gccgcgcatg cccgagccga cgccagtctg ccccgtatga aatagagccg
     1201 gcttgccagt ctcagcgatc gcttcgtaaa ggggatatgc gagtcgatcg ttcgggtaaa
     1261 aaccctgcat cgtggggtga aacttgaacc cctgcacgcc gaattcgttg accagccggc
     1321 gcgcctccct ggcaccgaac tttcccttgt gcgggtcaat cgacgcgaac ggtatcagta
     1381 tatcgctgtt ttcggccgcg atctcggcca cttcttcgtt cttatatcgg cgatatcctg
     1441 tttcgcgttc agcatcgaca gggaaaataa cagcggcgac attttgttcg cgataatgcg
     1501 ctgcggtctc gggc
//
LOCUS       DATLJT010000477         1852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1865524,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000477 DATLJT010000000
VERSION     DATLJT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865524"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..543)
                     /locus_tag="VM910_10995"
     CDS             complement(100..543)
                     /locus_tag="VM910_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63093.1"
                     /translation="MFPMQTKLPRIAFVAIFGGLAAFPTCAQSLQERMSTCFNCHGEK
                     GQSQRPEVPSLGAQPALYTLIELVMFRDQLRVTEPMNEMTKGLRACLKSPGLQSSPAK
                     DRFRVRHRPFSSGGGKGSNCPKAAARNLHRYRLSWVRSGRQGIAK"
     gene            complement(645..1370)
                     /locus_tag="VM910_11000"
     CDS             complement(645..1370)
                     /locus_tag="VM910_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63094.1"
                     /translation="MTTSKRTITIAAACVGLAALLTYAGAAQPLKVDFSDETVGGEPK
                     SFVSVVGVWRIENDGNNKVLVVDGRQWKEGQTAAGIADKARALYGDRYAEFLDRVQAY
                     AYFPYVVAKDVTDFRDGEISVRFEGISGRIDQGAGILFNLKPNGDYLTVRANPLENNL
                     VLWKFEKGKRSSVKWIRNTPTPTRQWHDLKVSIAGPKVAAYLDSKLYLEHALPEPVSG
                     RIGLWSKADSHMYFDDFTVTPAD"
     gene            complement(1367..>1852)
                     /locus_tag="VM910_11005"
     CDS             complement(1367..>1852)
                     /locus_tag="VM910_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63095.1"
                     /translation="RNSFPAADQRALADLREPLIITVHFAPEDPRYVDLRRNVLLKLE
                     RVMPHVTIRLATTGQSMVGSTSEEAYGEVEYSYGGRSDKSRSTSHREVLPLIYALAGK
                     PIPKPIAGEEYPGYPLLANASPALAWFLGGLPLLIVIAWWWSRRPPQIAPQLIKDGGQ
                     P"
BASE COUNT          349 a          552 c          585 g          366 t
ORIGIN      
        1 agatccagat cgacagtccg acgcaagtcg cggcctactg aggccggtgt gcggtcaaac
       61 gggacgcagt gcagttccaa ccggcgcacc gaaaatcatt catttcgcaa taccttgacg
      121 gccgcttctc acccaactga gtcgataccg atgcagatta cgcgcggcag ctttggggca
      181 atttgacccc ttcccgccgc ccgagctgaa cggccggtgt cgaactcgaa agcggtcctt
      241 cgctggagat gattgaagac cgggactttt gagacatgct cttagcccct tggtcatctc
      301 gttcatcggc tcggtaacgc gcagctggtc tcgaaacatg acgagttcga tcaacgtata
      361 aagtgccggc tgggcaccta atgaaggtac ttcggggcgc tgcgattgcc ctttctcgcc
      421 gtggcagtta aagcaggtag acatgcgctc ctgcagcgac tgcgcgcagg ttggaaacgc
      481 ggccaatccc ccaaaaatgg ccacaaatgc gatcctgggc aacttcgtct gcatcgggaa
      541 cattcgcaat cactcaaatt tcccgacgcc tgtctctgtc gccagagtga gcatcaccac
      601 gccgctgcaa tagcatgctg cccaaatcgg ggttgcacca tcgatcaatc tgctggcgtc
      661 accgtgaagt cgtcgaagta catgtggctg tcggccttgg accacaggcc gatgcgcccc
      721 gacaccggct cgggcagcgc atgctccaag taaagcttgc tgtcgagata ggccgcgacc
      781 ttgggcccgg cgatgctgac cttgagatcg tgccactggc gggtgggggt cggcgtattg
      841 cgaatccatt tcaccgagga ccgcttgccc ttctcgaatt tccaaagaac gagattgttc
      901 tccagaggat ttgcgcgcac ggtgagatag tcgccgttcg gcttgaggtt gaataggatc
      961 ccggcaccct gatcgatgcg gccagaaatg ccttcgaagc gcacggagat ttctccgtcg
     1021 cggaagtccg tgacgtcctt ggcgacgaca taggggaaat acgcgtaggc ctgaacccgg
     1081 tcgaggaatt cggcatagcg gtcgccgtag agcgcgcgcg ccttgtccgc gatgccggcg
     1141 gcggtctgtc cctctttcca ctgccggcca tcgacgacca gcactttgtt gttgccgtcg
     1201 ttctcgatcc gccagacgcc gacgacgctc acgaacgatt tcggctcgcc gccgacggtc
     1261 tcgtcggaaa aatcgacttt caggggttgc gccgcgccgg cgtatgtcag cagcgccgcg
     1321 aggccgacgc acgcggcggc gatggtgatg gtgcgcttgc tcgttgtcat ggttgacctc
     1381 cgtctttgat gagttgtggg gcgatttgcg gcgggcggcg gctccaccac cacgcaatga
     1441 cgatcagcag cggcaggccg ccgaggaacc aggcgagggc gggcgatgcg ttggcgagca
     1501 atgggtagcc cggatactcc tcgccggcga tcggcttcgg aatcggcttt cccgccagcg
     1561 cgtagatgag cggcaggact tcgcgatggc tggtcgagcg gcttttgtca gagcggccgc
     1621 cataggaata ctcgacctcg ccgtaagctt cctcgctcgt gctgcccacc atactttggc
     1681 ccgtggtagc gagccggatg gtgacgtggg gcatgacccg ctcgagcttg aggagaacat
     1741 tgcgccgcag gtcgacgtag cgcgggtctt ccggagcgaa atgaaccgtg atgatcagcg
     1801 gctcgcgcag gtcggcgagc gcgcgctgat cggcggcggg aaaggagttg cg
//
LOCUS       DATLJT010000478         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4393067,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000478 DATLJT010000000
VERSION     DATLJT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4393067"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VM910_11010"
     CDS             complement(<1..717)
                     /locus_tag="VM910_11010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVI63096.1"
                     /translation="MGARRAVSAIPAEVKGPTCFCRDCFADLDARARRCGECGSPRLV
                     RHRVLPKLAIAHIDCDAFYATVEKRDNPDIADKPVIIGGGKRGVVSAACYIARTFGVR
                     SAMPMFKALALCPSAIVIRPDMAKYVRVGREVRQAMQALTPLVEPLSIDEAFLDLSGT
                     ERVHGMIPAKVLARFAREVERNIGITVSVGLSCNKFLAKIASDLDKPRGFAALDQDEA
                     CAMLAEKPVGFIFGVGPATQE"
     gene            complement(696..995)
                     /locus_tag="VM910_11015"
     CDS             complement(696..995)
                     /locus_tag="VM910_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HVI63097.1"
                     /translation="MKNPVQNPREVAEIVAIQALNFIAGDADLLGRFLAETGIGPASL
                     RAAAADPNFLISVLDFVLRDDTTVKAFAKASELHPTNIAAAREILGDPRWERDVP"
     gene            1229..1594
                     /locus_tag="VM910_11020"
     CDS             1229..1594
                     /locus_tag="VM910_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI63098.1"
                     /translation="MAKTVLIVEDNELNMKLFRDLLEAHGYQTSGTSNGHEALDLVRK
                     MRPDLILMDIQLPQVSGLEVTRWIKDDPELRAIPVVAVTAFAMKGDEERIREGGCEAY
                     LSKPISVGKFIETVRRFIG"
     gene            1607..2980
                     /locus_tag="VM910_11025"
     CDS             1607..2980
                     /locus_tag="VM910_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HVI63099.1"
                     /translation="MSARILVVDDVPANVKLLEARLSAEYFDVLTATNGAEALSICQR
                     AECDIILLDVMMPDMDGFEVCRRLKSNPATHFIPVVMVTALDNPADRVRGLEAGADDF
                     LTKPVSDIVLLARVRSLTRLKMMTDELRMRALTSLEIGVQAPERSAVTDAGKGGRVLL
                     VDDRPSSYERLAPPLAEEHNVDVETNPAEALFQAADGDYDLLIVSLDLDNFDGLRLCS
                     QARSLERTRHVPILAIADPEHNTRLLRGLEIGVNDYLLRPVDKNELLARARTQIKKRR
                     YTDHLRDNVQHSIEAAITDALTGLHNRRYMESHLTTLAEQASSRGKPLALMMLDIDFF
                     KSINDTYGHDAGDDVLREFAVRIRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAE
                     RLRRSIAGEAFAIHKGAKRIEVTISIGLSTLERKGESVDDVLKRADNALYRAKHDGRN
                     RVVAQAA"
     gene            3244..>3428
                     /locus_tag="VM910_11030"
     CDS             3244..>3428
                     /locus_tag="VM910_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI63100.1"
                     /translation="MLDTTLDARTQAFLDKFEAALAAGDLDAAVAMFAPECYWRDLVA
                     FTWNIKTMEGRDQVREML"
BASE COUNT          681 a         1061 c         1058 g          628 t
ORIGIN      
        1 ttcctgggtc gctggcccca cgccgaagat gaagccgacc ggcttttctg cgagcattgc
       61 gcacgcctcg tcctgatcga gcgcggcaaa gcccctgggc ttgtcgaggt cggaggcgat
      121 cttggccaga aacttgttgc aggagagccc cacggaaacc gtgatcccga tgttgcgttc
      181 gacctcgcgg gcaaagcgcg ccagcacttt ggcggggatc atgccatgca cgcgctcggt
      241 gccggagaga tcgagaaagg cttcgtcgat cgacagcggc tcgaccagcg gcgtcagcgc
      301 ctgcatggcc tggcgcacct cgcggccgac ccggacatac ttcgccatat cggggcggat
      361 cacgatcgcc gagggacaaa gtgcgagcgc cttgaacatc ggcatcgccg agcgaacgcc
      421 aaaggtgcgg gcgatgtagc aggcggcgga gacgacgccg cgctttccgc cgccgatgat
      481 cacgggcttg tccgcgatgt cggggttatc gcgcttctcg acggtcgcgt agaacgcgtc
      541 gcaatcgata tgcgcgatcg caagtttcgg cagcacacgg tgacggacca ggcgggggga
      601 gccgcattcg ccgcagcgtc tcgccctcgc atccagatca gcaaagcagt cccggcagaa
      661 gcaggtgggg cctttcactt ctgccggaat agcactcacg gcacgtcgcg ctcccagcgg
      721 ggatcgccca ggatttcgcg cgcggccgca atattggttg ggtgcagctc cgatgccttc
      781 gcgaaagcct tcacggtcgt gtcgtcgcgg aggacaaaat cgagcacgga gatcaggaaa
      841 ttgggatcgg cggcagccgc ccgcagacta gcaggcccga ttccagtctc ggccaggaac
      901 cggcccagca gatcggcgtc gccggcgatg aagttcagcg cctgaatcgc aacgatttca
      961 gcgacttcac gagggttttg aacagggttt ttcaaccgcc gattgccttt ccgttaactt
     1021 atggtctcta atttgcaaac ccaccatgcc cgagtctctc gaacctgctc aagcaagtgg
     1081 aaaccacttc tcgcaaaaga agcaaactcg cttaacgggt tggaccgaat ctggcgctag
     1141 cttgaattca cattttggcg ggcttggccg cggctgcaag cgtagccgca aagccatagc
     1201 cggggataac agggattggg aggacgggat ggctaagacc gtcctgatcg tggaggacaa
     1261 cgagctcaac atgaagctct ttcgcgatct gttggaagca cacggctacc agacctcggg
     1321 caccagcaac ggccatgagg cgctcgatct cgtgcgcaag atgcgtcccg acctgatcct
     1381 gatggacatc cagttgccgc aagtgtccgg cctggaggtc acgcgctgga tcaaggacga
     1441 tccggaattg cgcgccattc ccgtcgttgc cgtcaccgcc tttgcgatga agggcgacga
     1501 ggagcgaatc cgcgaaggcg gctgcgaggc ttatttgtcc aaaccgattt cagtcggcaa
     1561 attcatcgaa acggttcgtc gattcatagg ttaggagaga gttttagtgt ccgcgcgtat
     1621 cctggtcgtc gacgacgtcc ccgccaacgt caagttgttg gaagcccgcc tgtcggccga
     1681 atattttgac gtcttgaccg caaccaacgg tgccgaggcg ctgagtatct gtcagcgcgc
     1741 cgaatgcgac atcatcctcc tcgacgtgat gatgcccgac atggacggat tcgaggtctg
     1801 ccgtcggctg aagtcgaacc ctgccacgca tttcattccc gtcgtcatgg tgaccgcgct
     1861 cgacaacccc gccgatcgtg tgcgcgggct cgaagccggc gccgacgatt tcctgaccaa
     1921 gcccgtatcc gacatcgtcc tgcttgcgcg cgtgcgctcg ctgacgcggc tgaaaatgat
     1981 gaccgacgaa ttgcgcatgc gtgcgctcac ctcgctcgag atcggggtgc aggcgccgga
     2041 acgcagcgcg gtaaccgacg ccggcaaggg cgggcgcgtc ctgctggtcg acgatcgtcc
     2101 gtcttcctac gagcggctcg ctccgccgct tgccgaagag cacaacgtcg atgtcgagac
     2161 caacccggcg gaggccttgt ttcaggctgc cgacggcgac tacgaccttt tgatcgtctc
     2221 gctcgatctc gacaattttg acggcttgcg gctctgcagc caggcgcgct cgctggaacg
     2281 gacacggcac gtgccgatcc ttgcgatcgc cgatcccgag cacaataccc gcctgttgcg
     2341 cgggctcgag atcggcgtca acgactatct gttgcgcccg gtcgacaaaa acgagctgct
     2401 ggcacgggcc cgcacgcaga tcaagaaacg ccgctacacc gatcacctcc gcgacaacgt
     2461 acagcattcg atcgaggccg cgatcaccga cgcgctgacc gggctgcata accgccgcta
     2521 catggaaagc catctgacga cactggccga acaggcctcc agtcgcggca agccgctggc
     2581 gctgatgatg ctcgacatcg attttttcaa gtcgatcaac gacacctatg gccacgacgc
     2641 cggcgacgac gtgttgcgcg agttcgccgt ccgtattcgc aaatcgatcc gcggcatcga
     2701 tctcgcctgc cgctatggcg gcgaggagtt cgtgatcgtg atgccggaga cggatctgca
     2761 cgtcgccggc atggtggcgg aaaggctgcg ccgttcgatc gcgggcgagg cctttgcgat
     2821 ccacaaggga gccaagcgca tcgaggtcac gatctcgatc gggctgtcga cgctggagcg
     2881 caagggcgag tcggtcgacg acgtcttgaa gcgcgccgac aacgcgctct atcgcgccaa
     2941 gcatgatggc cgcaaccgcg tggtcgcgca ggcggcgtga gcccgtcatc tcgattttga
     3001 attcctccat cattgcgcgg gtcgtcgccc gccgcttctc gcaatgacga gcctcctttc
     3061 cgattcaagc tgccaagggt tttgctaccc catcggtggg cgaagcagcg ccttcttggg
     3121 agagcgctga gaagcgcaaa agccggaccg caaattcgga ccgcgcaagt ctggaatcgt
     3181 ccggtagtta ggataaggtc gggagattgc agaaaaactg aaaaaacgac tcgggaggaa
     3241 atgatgctcg acaccacgct tgacgcgcgg acccaggcct ttcttgacaa attcgaagca
     3301 gcccttgcgg caggcgatct cgacgccgcc gtagcgatgt tcgcaccgga atgctactgg
     3361 cgcgacctcg ttgccttcac ctggaacatc aagaccatgg agggccgcga tcaggtccgc
     3421 gagatgct
//
LOCUS       DATLJT010000479         7571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4163968,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000479 DATLJT010000000
VERSION     DATLJT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4163968"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VM910_11035"
     CDS             <1..609
                     /locus_tag="VM910_11035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HVI63101.1"
                     /translation="DDGAEIRMEFTVATGKGYVPAERNRPEDAPIGLIPVDSLFSPVR
                     KVSYKVENTREGQILDYDKLTMTIETNGAITPEDAVAFAARILQDQLNVFVNFEEPRK
                     EVTQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDLVQKSEAEMLRTPNF
                     GRKSLNEIKEVLAQMGLHLGMEVPGWPPENIDELAKRFEDHY"
     gene            747..1166
                     /gene="rplQ"
                     /locus_tag="VM910_11040"
     CDS             747..1166
                     /gene="rplQ"
                     /locus_tag="VM910_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVI63102.1"
                     /translation="MRHGRVYRKLNRTAEHRRAMFANMAAALIKHEQIVTTLPKAKEL
                     RPIVEKLFTLGKKGGLAMRRQAISELRDVDMTRKLFDTLAPRYKDRHGGYTRIIKAGF
                     RYGDNAAMAVIEFVDRDVEAKGQDSGPAAEKAPAEAA"
     gene            1301..1423
                     /locus_tag="VM910_11045"
                     /pseudo
     CDS             1301..1423
                     /locus_tag="VM910_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            1528..2034
                     /locus_tag="VM910_11050"
     CDS             1528..2034
                     /locus_tag="VM910_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63103.1"
                     /translation="MIALRQAILSLCVALLVVWPHSPARAEQAFERLLPLLVDLDGWQ
                     GKKPDGMSMQMSKVNVTTATRDYQRGSAQVHASVMVGQAAAGALAPLQKGINLQTSDG
                     HTVTATMHGMPVLKNAITSQKSGTLMVELGKDALFSLAYSGITEAEALELAEKFDWKA
                     IQAATEAK"
     gene            complement(2039..3052)
                     /locus_tag="VM910_11055"
     CDS             complement(2039..3052)
                     /locus_tag="VM910_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HVI63104.1"
                     /translation="MKAVGYRKSLPIEDKNALIDFETAKPEPKGRDIRVAVKAISANP
                     VDYKVRKRAAPPEGEYKILGFDAAGIVDAVGPDVSLFKPGDEVFYAGSIQRQGTNSEF
                     HLVDERIVGSKPKTLSFAQAAALPLTSLTAWELLFDRLGAVPGKSLDPRTLLIVGGAG
                     GVGSILTQLARRLTGLTVVATATRPDSQKWCLELGAHAVVDHAKPLKEQIDKLKVPPV
                     ALVASLTATDQHYKGIAELMAPQGKYGLIDDPAEFNVAVFKGKAISIHWESMFTRSSF
                     QTADMIAQHNLLNDVADLIDKGVLRTTLAQTFGTINADNLKRAHALLESGKSRGKIVL
                     EGW"
     gene            3179..3565
                     /locus_tag="VM910_11060"
     CDS             3179..3565
                     /locus_tag="VM910_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI63105.1"
                     /translation="MKRQNFAHRPGCAVEATLDLIDGKWKGVILFHLQSGTQRFGELR
                     RRMPGITQRMLTKQLRALEDDRLVIRKVYAEVPPRVEYCLSEIGESLRPVIDTLRAWG
                     ESHQERLSCAPPEITKQTNKASQHVV"
     gene            3630..5024
                     /locus_tag="VM910_11065"
     CDS             3630..5024
                     /locus_tag="VM910_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HVI63106.1"
                     /translation="MKLICSLAVLLLSAAFASPTLAQDRRVPTSPSDIKLSYAPIVQR
                     VQPAVVNVYAAKIVQNHNPFLDDPIFRRFFGLPGGQQEQMQRSLGSGVMVDPSGLVVT
                     NVHVIEGADQVKVSLSDKREFEAEIMLKDSRSDLAVLRLKDSHEKFPTLELANSDELM
                     VGDVVIAIGNPFGVGQTVTHGIISALARTQVGITDYQFFIQTDAAINPGNSGGALVDM
                     TGRLVGINTAIYSRSGGSQGIGFAIPANMVRVVVASAKSGGKAVKRPWLGARLQAVTP
                     EIAESLGLKTPSGALVASIVPNSPAARAGLKSSDLITAIDGQAIEDANAFDYRFATHP
                     LGGTSQIDVQRGGKAVRLTVPLETAPDTGRNEIVLSSRSPFQGAKVANISPAVADELH
                     LDADTEGVVITELGDDSTAANVGFQKGDVILAVNNTKVGKTSDLEKATSQSTRVWRIT
                     LMRNGQQINVTLGG"
     gene            5021..6358
                     /locus_tag="VM910_11070"
     CDS             5021..6358
                     /locus_tag="VM910_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HVI63107.1"
                     /translation="MSPKRPQQQGSTLFAAAGMDQDAPRPLPDRLRPRTLAEVVGQDH
                     ILGADGALTRMLETRTLGSLIFWGPPGTGKTTVARLLADATDLHFEQLSAVFSGVADL
                     KKAFDAARARRETGRGTLLFVDEVHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFEL
                     NAALLSRARVLVFHSLDAAAIEKLFAHAEQVEGKKLPLDAEARAVLVRMSDGDGRAAL
                     TLAEEVWRSARGGEIFNAAQLQDILQRRAPIYDKSADGHYNLISALHKAVRGSDPDAA
                     LYYLARMLDAGEDPLFLARRVVRMAVEDIGLADPQALVVANAAKDAYDFLGSPEGELA
                     IAQAVVYVATAPKSNAVYTAFGAAKRTAKEAGSLLPPKHILNAPTRLMQEEGYGAGYE
                     YDHDAPDAFSGRDYFPEALGRQTFYDPPERGFEREIRKRLDYWAKLRKERGGK"
     gene            6415..>7571
                     /locus_tag="VM910_11075"
     CDS             6415..>7571
                     /locus_tag="VM910_11075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVI63108.1"
                     /translation="MSRRFKRTVSPADRGKKERADRRDGKRAKGHRVPEHRGGGKPPR
                     FSSEHRESRAPIANPVKDKPEPQLPTKVQTVVVTPDEDNMRVDRFLEARFPGLSFSHI
                     QRIVRKGELRVNGKRADSKDRLQAGQSIRIPPLRLDAPKAGGALSEAEEKTLQALKAM
                     TLYEDEDVLVLNKPAGLAVQGGSGTTRHVDQMLEVMRDAKGQKPRLVHRLDKGTSGCL
                     LVAKTRFAATHLTGAFRHRSARKIYWALVAGVPKPKQGRISTFLAKEEGEEDTIMRIA
                     AHGEEGASHAVTYYAVVETAANKLAWVSLKPVTGRTHQLRAHMAHIDHAIIGDPKYFT
                     KENWELPGGLQNRLHLLARRIAIPHPRGGVIDVTAPLPPHMLQSWNLLGLEA"
BASE COUNT         1446 a         2317 c         2426 g         1382 t
ORIGIN      
        1 gacgacggcg cggagatccg catggagttc acggtcgcga ccggcaaggg ctatgtgccc
       61 gccgagcgca atcggcccga ggacgccccc atcggcctga tcccggtcga tagcctgttt
      121 tcgcccgtgc gcaaggtttc ctacaaggtc gagaacaccc gcgagggcca gattctcgac
      181 tacgacaagc tgaccatgac gatcgagacc aacggcgcga tcacgccgga ggatgcggtc
      241 gcgttcgctg cacgtatcct gcaggatcag ctcaacgtgt ttgtcaactt cgaagagccg
      301 cgcaaggaag tcacgcagga gattatcccc gatctcgcct tcaacccggc cttcctcaag
      361 aaggtcgacg aactcgagct gtcggtgcgc tcggcaaact gcctgaagaa cgacaacatc
      421 gtctacatcg gcgatctcgt gcagaagtcg gaagcggaga tgctgcgcac tccgaacttc
      481 ggtcgcaagt cgctgaacga gatcaaggaa gtgctggccc agatgggcct gcatctcggc
      541 atggaagtgc cgggctggcc gccggagaat atcgacgagc tcgccaagcg cttcgaggat
      601 cattactgat ccagtttcac cgtcgtggcc gggagctccc ggccatcaca cagcgccatc
      661 gcgctttagc gggcgaacgc aggcagccca cctgagcaaa gtgtccgacg aaccgtcgtg
      721 gcagacagaa agcaaggaat agagtcatgc gtcacggacg agtttatcgt aagctcaatc
      781 gcactgccga gcatcggcgc gcgatgtttg caaacatggc tgctgcgctg atcaagcacg
      841 agcagatcgt caccacgctg ccaaaggcca aggagctgcg gccgatcgtc gagaagctgt
      901 ttacgctcgg caagaagggc gggctcgcga tgcgccggca ggcaatctcg gagctgcgcg
      961 acgtcgacat gaccaggaag ctgttcgaca cgctggcgcc ccgctacaag gaccgccacg
     1021 gcggctatac ccgcatcatc aaggcgggct tccgctacgg cgacaatgcg gcgatggcgg
     1081 tgatcgagtt tgtcgatcgc gatgtcgagg ccaagggcca ggactccggc cccgccgccg
     1141 agaaggcgcc cgcggaagcg gcctgagccc ttcaggtctc agcgttcatt ggcggcgccc
     1201 atccgggcgc cgctttttta tcgaggccgg cccccggcat ttttgccgat tgcggtggcg
     1261 cgccccgttg ccgatatctg cggtgacggc cgtggcccac gagcgctttg cgggcgtcga
     1321 ggaggtcgcc aacatggtgg tctttgtcgc gtcgaaagag gtttgcgccg ccaacagcgc
     1381 cgtactgcgc gccgaaggcg gcatcgtcaa caccattgcc tgagatacta tagttacgcc
     1441 ccgagggctt gagccggtca gacttcgcgg tagatcgcgc caagacgtga cgctatgatc
     1501 ctgcgccgat cacgggggga gtcgcaaatg atcgcgctgc ggcaggcgat tctttcgctt
     1561 tgtgtggcac ttttggtggt gtggccgcat tctccggcgc gcgccgagca ggcttttgag
     1621 cgcttgctgc cgcttttggt cgatctcgac ggttggcagg gcaagaagcc ggacggcatg
     1681 tcgatgcaaa tgtccaaagt caacgtgacc acggcgaccc gcgattatca gcgcggctcg
     1741 gcgcaagtgc atgcgtcggt catggtaggc caggccgcgg cgggcgcgct ggcgccgtta
     1801 cagaagggga tcaacctcca aacctccgac ggccacacgg tcactgcgac catgcacggc
     1861 atgcccgtct tgaaaaacgc catcacgtca cagaagtcag gcacgctgat ggtcgagctc
     1921 ggcaaggacg cgctgttcag cctggcctac agcggcatta ccgaggcgga ggcgctggag
     1981 ctcgctgaaa agttcgattg gaaagcgatc caggcggcca cggaggcaaa gtagcgagct
     2041 accatccctc cagcacgatc ttgccgcgcg acttgccgct ttcgagcagg gcatgcgcgc
     2101 gcttgaggtt gtcggcgttg atcgtgccaa aggtctgggc gagcgtggtg cgaagcacgc
     2161 ctttgtcgat gagatcggcg acatcgttca gcaaattgtg ctgggcgatc atatcggccg
     2221 tctggaacga ggagcgggtg aacatcgatt cccaatgaat cgagatcgcc ttgcccttga
     2281 acaccgcgac gttgaactcg gcgggatcgt cgatcagccc gtatttgccc tggggcgcca
     2341 tcagttcggc gatccccttg tagtgctggt cggtggcggt gaggcttgcc accagcgcaa
     2401 cgggcggcac cttcaattta tcgatctgtt ccttcaacgg ctttgcgtga tcgaccacag
     2461 catgcgcgcc aagctcgaga caccatttct gggagtcggg ccgtgtagcc gtggcaacca
     2521 ccgtcaatcc ggtaagcctg cgcgcaagct gggtcaggat cgagccgacg ccacccgcac
     2581 cgccgacgat gagaagcgtt cgcggatcaa ggctcttgcc gggcaccgcg ccgagccgat
     2641 cgaacaagag ttcccaggcc gtcagcgagg tcagcggcag cgccgctgcc tgcgcgaaag
     2701 acaatgtttt tggcttgctg ccgacgatgc gttcgtcgac caggtgaaat tccgagttcg
     2761 ttccctggcg ctggatcgag ccggcgtaga agacctcgtc gcccggtttg aacaggctga
     2821 cgtcagggcc gaccgcatcg acaatgccgg ccgcatcgaa gccgagaatc ttgtactcgc
     2881 cttcgggcgg tgccgcgcgc ttgcgaacct tgtaatccac gggattcgcc gaaatggcct
     2941 tcacagcgac acgaatgtca cgccctttcg gttccggctt cgcggtctcg aaatcgatca
     3001 gagcattctt gtcctcgatc ggcagcgact tccggtatcc gacggccttc atggagtttc
     3061 tcccgcacaa cgcttgtgga atctaattgt cgcgctggtc gcaatttggc aagtactgta
     3121 aattcaggga tatagtccca gtttggatac tattggggaa aatgtcgaga aaacactcat
     3181 gaaacggcag aatttcgcgc atcgaccggg ctgcgcggtc gaggcgacgc tcgacctgat
     3241 tgatggcaag tggaagggag tgatcctgtt tcacctgcaa agcggtaccc aacgctttgg
     3301 tgaattgcgg cggcggatgc ccgggattac ccagcgcatg ctgaccaaac agctccgtgc
     3361 gctggaggat gaccggctgg tcatccgcaa ggtctatgcc gaggtgccgc cgcgggtgga
     3421 gtactgcttg tcggagatcg gcgagagctt gcggccggtg atcgatacgc tgcgagcctg
     3481 gggtgagagt catcaggaac ggctatcctg cgcaccaccc gagatcacca agcaaaccaa
     3541 caaggcctcg cagcacgttg tgtagcggcc gccgcgttcg caaaccatat cccggcccgt
     3601 ttgggccaac tgctttttta ggatttcgca tgaagttgat ctgctctctg gctgtcctgc
     3661 tcttatccgc tgcttttgcg tcgcccacat tggcgcaaga ccgtcgcgtg ccgacgtcgc
     3721 cctcggacat caagctctcg tatgcgccga tcgtgcagcg cgtgcaaccg gcggttgtca
     3781 acgtctatgc cgccaagatc gtccaaaacc acaatccgtt tctggatgac ccgatcttcc
     3841 gccgcttctt tggcctgccg ggcggccagc aggagcagat gcaacgctcg ctgggctccg
     3901 gcgtgatggt ggatccgtcc ggcctcgtcg tcaccaacgt gcacgtcatc gaaggtgccg
     3961 atcaggtgaa ggtctcgctg tcggacaagc gcgagttcga ggccgagatc atgctgaagg
     4021 acagccgctc cgatctcgcg gtactgcggt tgaaggattc gcatgagaag tttccgacgc
     4081 tcgaactcgc caattccgat gaactgatgg ttggcgacgt ggtgatcgcg atcggcaacc
     4141 ctttcggcgt cggccagacg gtgacgcacg gcatcatctc ggctctcgcg cgcacgcagg
     4201 tcggcattac tgattaccag ttcttcattc agaccgatgc ggcgatcaat cccggcaatt
     4261 ccggcggtgc gctggtcgac atgacggggc ggctggttgg catcaacacc gcgatctatt
     4321 cgcgctccgg cgggtcgcag ggcatcggct tcgcgatccc ggccaacatg gtgcgcgtgg
     4381 tggtggcttc cgccaagagc ggcggcaagg cggtgaagcg gccgtggctt ggtgcgcggt
     4441 tgcaggcggt gacgccggag atcgccgaaa gcctcggatt gaaaacaccc tcgggcgcgc
     4501 tggtcgcaag catcgttccc aatagtccgg ccgcacgcgc cgggctgaaa tcatccgatc
     4561 tgatcaccgc gatcgacggg caggcgatcg aggatgccaa tgcgttcgac taccgctttg
     4621 ccacgcatcc gcttggcggt acctcgcaga tcgacgtgca gcgtggcggc aaggcggtca
     4681 ggctgacggt gccgctcgag actgctcccg ataccggccg caatgaaatc gtgctgagct
     4741 ctcgttctcc gttccagggc gcgaaagtcg ccaatatctc gccggccgtt gccgacgaat
     4801 tgcatctgga tgccgacacc gaaggcgtcg tcatcaccga actcggcgac gattcgaccg
     4861 ccgccaatgt aggcttccag aagggagacg tcattctggc tgtgaacaac acgaaagttg
     4921 gaaaaaccag cgaccttgaa aaggcgacaa gccagtcgac gcgggtctgg cgcatcacgc
     4981 tgatgcgcaa tggccagcag atcaacgtca cgctcggcgg atgagtccga agcgaccaca
     5041 gcagcaagga tcgaccttgt tcgcggccgc agggatggat caggacgcgc cgcggccgct
     5101 tcccgatcgg ttgcgtccgc gcacgctcgc cgaggtcgtc ggtcaggatc acatcctggg
     5161 agctgacggc gcgcttacgc gcatgctgga gacccgcacg ctcggctcgc tgatcttctg
     5221 gggcccgccg ggcaccggca agaccaccgt ggcgcggctt ttggccgatg cgaccgattt
     5281 gcatttcgag cagctttcgg cagtgttttc cggcgtcgcc gacctgaaga aagcgttcga
     5341 tgcggcgcgc gcgcggcgcg agacgggcag gggcacgctg ctgttcgtcg acgaggtgca
     5401 caggttcaat cgcgcccagc aggactcgtt cttgcccgtg atggaagacg gcactgtggt
     5461 gctggtcggc gcgaccaccg aaaacccttc gtttgaactc aacgcggcgc tgttgtcgcg
     5521 ggcgcgcgtc ctcgtatttc attcgcttga cgcggccgcg atcgagaagc tctttgccca
     5581 cgccgagcag gtggaaggca agaaactacc gcttgatgcg gaagcacgcg ccgtattggt
     5641 gcgcatgtcc gacggcgacg ggcgtgctgc cctcacgctg gccgaagagg tctggcggtc
     5701 ggcgcggggc ggggagattt tcaatgccgc gcaattgcag gacattctgc agcggcgcgc
     5761 gccgatctac gacaaatccg ccgacggtca ctacaacctg atctccgcgc tgcataaggc
     5821 ggtacgtggc tccgatcccg atgccgcgct ctattacctc gcgcgcatgc tcgatgccgg
     5881 cgaagacccg ctgttcctcg cgcgccgcgt ggtgcgcatg gcggtggagg atatcgggct
     5941 tgccgacccg caggcgcttg tcgtcgccaa tgcggcgaag gatgcctacg attttctcgg
     6001 ctcgcccgag ggcgagctcg cgatcgcgca agctgtggtc tatgtcgcaa ccgcgcccaa
     6061 gtcgaatgcc gtctatacgg ctttcggcgc cgccaagcgc acggccaagg aggccggctc
     6121 gctgctacca ccaaagcaca tcctgaatgc gccgacccgg ctgatgcagg aggagggcta
     6181 tggcgcgggc tatgagtacg accatgacgc gccggacgcc ttttccggcc gggactattt
     6241 cccggaggcg ctggggcgcc agaccttcta tgatccgccc gagcgcggct ttgagcgcga
     6301 gatcaggaaa cggctcgact attgggcgaa gctgagaaaa gaacgcggcg ggaaatagcg
     6361 tcgctttttg agaaacgccg tgacgaccgc tctcttttcc gctaggcacc cggcatgagc
     6421 cgacgcttca agagaacagt gtcccccgct gatcgcggca agaaggagcg cgctgatcgc
     6481 cgcgatggta agcgcgcgaa gggccatcgt gtgcccgagc atcggggcgg cggaaagcca
     6541 ccgcgcttct cttccgagca tcgcgagtca cgcgcgccca tcgcaaatcc tgtgaaggac
     6601 aagcccgagc cgcagcttcc gacaaaagtc cagaccgtcg tggtgacgcc ggacgaggac
     6661 aatatgcggg tcgaccgctt tctcgaggca cgctttccgg ggctgtcgtt ctcacatatc
     6721 cagcgcatcg tccgcaaagg cgaactgcgc gtgaacggca agcgcgccga cagcaaggat
     6781 cggcttcagg cggggcagag catccgcatt ccgccgctca ggctcgacgc gccgaaggcg
     6841 ggcggtgcgt tgtcggaggc ggaagagaag acgctgcagg cgttgaaggc gatgacgctt
     6901 tatgaagacg aggacgtgct ggtgctgaac aagcccgccg gcctcgccgt acagggcggc
     6961 tcgggcacga cgcggcatgt cgaccagatg ctggaggtga tgcgcgatgc caagggccag
     7021 aagccacgcc tggtgcatcg gctcgacaag ggaacgtcgg gctgcctgct ggttgccaag
     7081 acgcggttcg cggcgacgca tctgaccggc gccttccgcc atcgttctgc gcgcaagatc
     7141 tattgggcgc tggttgccgg tgtgccgaag ccgaagcagg gccgcatctc gacttttctc
     7201 gcgaaggaag agggtgagga ggacacgatc atgcgcatcg ccgcgcatgg cgaggagggc
     7261 gcaagccacg ccgtcaccta ttatgcagtc gtcgagaccg cagcgaacaa gctcgcttgg
     7321 gtatcgctaa aaccagtcac ggggcgcacc catcaattgc gggcgcatat ggcccatatc
     7381 gatcacgcca tcatcggcga tcccaaatat tttaccaagg agaactggga gcttcccggt
     7441 ggcctgcaga atcggttgca tctgttggcg cgccgcattg cgattccgca ccctcgcggc
     7501 ggcgtgatcg acgtgaccgc gccactcccg ccgcatatgc tacaatcgtg gaacttgctc
     7561 ggcctggaag c
//
LOCUS       DATLJT010000480         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1865722,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000480 DATLJT010000000
VERSION     DATLJT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865722"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VM910_11080"
     CDS             <1..1101
                     /locus_tag="VM910_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI63109.1"
                     /translation="AINVGDTTIMLPGMGSYIALAIEQRSLAAVGWAIGTMLVVILIY
                     DQLLFRPLVAWADRFRFEQEVGVAPPKSWVLSTLRRSGVVATLTRPLSVAWQRSFQMP
                     PLGAGSKLARARSQERPSWSDRGWTALIVTIAALALWQVGRFILAGVSLTDVGKAVLL
                     GLATLTRVIVLISLASLIWVPIGVWVGTRPRAARIFQPVAQFLAAFPANLLFPVVVSA
                     IVVLKLNPDIWLSPLMILGTQWYILFNVIAGATAIPSELRDCGTNLGVCGWLWWRKIA
                     LPAVFPYYVTGAITASGGSWNASIVAEVASWGDERLQAYGLGAYIAQATEAGDFHRIV
                     LGIAVMSLFVVVINRTFWRPLYRYAERKFRLG"
     gene            1113..>2044
                     /locus_tag="VM910_11085"
     CDS             1113..>2044
                     /locus_tag="VM910_11085"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63110.1"
                     /translation="MMSEAPLLELHSVRQSFPKPGGNQLLVLDDINVELAPGQIVGLL
                     GRSGSGKSTLLRLIAGLARPTAGSITYLGRPIVSPAPGIAMVFQSFALFPWLTVLENV
                     QLGLEALGLPETEIRQRALAAIDLIGLDGYESAYPRELSGGMRQRVGFARALVVHPNI
                     LLMDEPFSALDVLTAETLRTDFLDLWSEGRLPIKGVILVTHNIEEAVLMCDRILVFST
                     NPAQIVSEIKVDLPQPRNRLDPRFRDLVEGIYVAMTERSTAAARATPLPERFPKIGTD
                     TVLTRVSANLLSGLIEAVAGPPYNGKADLPVIASS"
BASE COUNT          323 a          610 c          629 g          482 t
ORIGIN      
        1 gcaatcaacg tcggcgacac cacgatcatg ctgccgggca tgggctctta catcgcgctt
       61 gcgattgagc agcgaagtct cgcggccgtt ggttgggcga tcggcaccat gctggtggtc
      121 atcttgatct atgaccaact cttattccgg ccgttggtcg cctgggcgga ccgcttccgg
      181 ttcgagcagg aagtaggcgt cgcgcctccc aaatcgtggg tcctcagcac actgcgtcgc
      241 tctggcgtcg tcgcgaccct gacccgtcca ttgtcggtgg cgtggcagcg ctcttttcag
      301 atgccgccgc tcggcgcagg cagcaagctc gcgcgagccc gatcgcaaga gcgcccgagt
      361 tggtctgatc gcgggtggac cgcgcttatc gtgaccattg cagcactcgc gctttggcag
      421 gtcgggcggt tcatccttgc aggcgtttcg ttgacggatg tgggcaaggc ggtccttctc
      481 ggcctggcca cgctgacacg cgttatcgtt cttatctcgc ttgcgagcct gatctgggta
      541 ccaattggtg tttgggtggg aacgcgtccg cgcgcggcgc gtatctttca gcccgttgcc
      601 cagttcctgg cagcctttcc agcaaatctt ttgtttcccg tcgtcgtgtc ggcgatcgta
      661 gttctaaaac tcaatcccga catctggctt agtcctctga tgattcttgg cacccagtgg
      721 tacatcttgt tcaacgtcat cgccggtgcc acggcgatcc cctcggaact gcgcgattgc
      781 gggaccaatt tgggcgtctg cggctggctg tggtggcgga agatcgctct gccggctgtg
      841 ttcccgtact atgtaaccgg tgcaatcacc gcgtctggcg gctcgtggaa cgcgagcatc
      901 gtcgccgaag tcgcgtcctg gggtgatgag cgcctgcagg cctatggtct cggtgcatat
      961 atcgcgcagg cgacggaggc aggggatttc catcgaattg tgcttggcat tgcggtgatg
     1021 agcttgttcg tggtcgttat taacaggacg ttctggcgtc cgttgtaccg gtacgccgaa
     1081 cggaagtttc ggctgggata gaaatggaga cgatgatgag tgaggctccc ctgctggaac
     1141 tccattccgt acgccagtcc ttccccaagc ctggcggcaa tcagctgctc gttctcgacg
     1201 acatcaacgt ggagctcgcg ccggggcaga tcgtcggcct tcttggccgg tcggggtcgg
     1261 gcaaatcgac tttgcttcgc ttgatcgctg ggcttgcacg cccgactgca ggaagcatca
     1321 cgtatctcgg caggccgatc gtttctcctg cgcctggcat tgcgatggtg tttcagtcct
     1381 tcgcgctttt tccctggctc accgtgctgg aaaatgtcca gctcggattg gaggcgctcg
     1441 gccttcccga gacggagatt cgccagcgtg cgcttgccgc gatcgacttg attggcctgg
     1501 acggctacga gtcggcctat ccacgcgagc tgtccggcgg catgcgtcag cgggttggct
     1561 tcgcgcgcgc gctcgttgtt catcccaata ttctgttgat ggatgaaccg ttctcggcgc
     1621 tcgacgtcct caccgcggaa actctgcgga cggactttct cgatctctgg agcgaaggcc
     1681 gcctccccat caaaggggta atcctggtaa cgcataatat tgaagaagcg gtactgatgt
     1741 gcgaccggat cctcgttttc tcgaccaatc cggcgcagat cgtgagcgag atcaaggttg
     1801 acttgccgca acctcggaac cgccttgatc ccaggttccg tgatcttgtc gagggaatct
     1861 atgtcgccat gacggagcgt tctaccgcgg cggcgcgcgc tacccctctg ccggaacgct
     1921 ttcccaagat tgggaccgac acggtgctga cgcgggtctc cgccaatttg ctctccggtc
     1981 tgatcgaggc ggtcgccggc ccgccatata atggaaaggc cgaccttccg gtcattgcaa
     2041 gttc
//
LOCUS       DATLJT010000481         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2325464,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000481 DATLJT010000000
VERSION     DATLJT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325464"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VM910_11090"
     CDS             complement(<1..126)
                     /locus_tag="VM910_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVI63111.1"
                     /translation="MATGTVKFFNTQKGFGFIQPTDGSKDVFVHISAVERAGMRTL"
     gene            complement(310..558)
                     /locus_tag="VM910_11095"
     CDS             complement(310..558)
                     /locus_tag="VM910_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63112.1"
                     /translation="MTFDYDAPAELFMAKRKGGSRQRLGYRRFATAAEAIRFAVEDFP
                     AVRTLGAWLQVGDERFDGDAIQRLYDSGNYPLQRRPSN"
     gene            complement(715..912)
                     /locus_tag="VM910_11100"
     CDS             complement(715..912)
                     /locus_tag="VM910_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63113.1"
                     /translation="MSDHKFKVGQSVLYTSGPFGRGATSGVYKVTHLLPPQGDDHQYR
                     IKSSDEPHERVVKESELHRES"
     gene            1107..1787
                     /locus_tag="VM910_11105"
     CDS             1107..1787
                     /locus_tag="VM910_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HVI63114.1"
                     /translation="MHSSTHAALALLLLAAQGAHAQAPLPAGFVDAAAVVDGLVVDMR
                     YFGANNFVGERIDGYECPRCLLSAPAANALAAVERDLAARGLGLKVFDCYRPQRAVAQ
                     FVRWAQRIDDVKRKREFYPDVDKRDLFKEGYISDRSGHSRGSTLDLTLVRRADARELD
                     MGSPFDFFSPKSWPSDRSVSDQAQQNRALLAAVMIRGGFRPYDREWWHFTLNNEPFPD
                     TYFDFPVR"
     gene            complement(1883..2416)
                     /locus_tag="VM910_11110"
     CDS             complement(1883..2416)
                     /locus_tag="VM910_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63115.1"
                     /translation="MASERQIAANRRNARKSTGPRSGAGKKRASRNAYRHGLTLSITS
                     SAAVAKQLDTLVRKIAGDSEDAILLERARAIAHAELDLARVRRAKVALIERASAFGEL
                     DPPQLTVTQMIRVLNAFERGRPIPKPIDALATMPSQETDRSAEAIRRVLPELRKLDRY
                     ERRAAARRDRAVLFYSS"
BASE COUNT          423 a          770 c          807 g          500 t
ORIGIN      
        1 gagcgtgcgc atgccggcgc gctcgaccgc gctgatgtgc acgaatacgt ctttgctgcc
       61 gtcggtcggc tggatgaatc caaagccttt ttgagtgttg aagaacttca cggtacctgt
      121 ggccatcatg gcctccttgc gctgtacgct atgtgaatcc gatcacgcga acatcgcgcg
      181 ccgggtcatc cgagttttgg gagctggccg agcacccgaa acgggagcgt agcaaggtag
      241 accgaaaact ggttgcggcc gctgtatatg tgagcgcggc cgcgacgtca aaccggggtt
      301 accggttcgt tagtttgacg ggcggcgttg caacggataa ttgccgctgt cgtagagacg
      361 ctgaatggcg tccccgtcga aacgttcgtc gccgacctgc agccaggcgc ccagcgtgcg
      421 cacggcgggg aaatcctcga ctgcgaaacg aattgcctcc gccgccgtcg caaagcggcg
      481 atagcccagc cgctgtcgcg acccgccttt gcgcttagcc atgaacaact cggctggtgc
      541 gtcgtagtcg aatgtcatgg tcttgctttc gaccggaata tcgatcgcct catgcgagtt
      601 gcaaacgcgc cgatcacacc catccggacc aaggcgacag gcagctctcc atgggttgcc
      661 cggggcgcgg ctcgcggggg ccattcgaag caagcggggc atgccgcctc gccgtcacga
      721 ctcgcggtgg agctcacttt ccttgacgac gcgctcatgc ggctcgtcgg aactcttgat
      781 gcgatattga tggtcgtccc cttggggcgg aagcagatgc gtgactttgt agacgccgct
      841 ggtcgcgccg cgaccgaaag gcccggaggt gtaaagaacg ctctggccga ccttgaactt
      901 gtgatcgctc acgtctctct ctccgtgctg cagaaaacgc gcggtccacc gccgctgcgg
      961 cggggccgaa cgcctctctt gttggattca tgctgaattc tatcatggaa tgcgcgggaa
     1021 tgcgactatt tgtgagatag gcgtgccggc cgccgatgca ccaaagccat ctcggcgcgg
     1081 accggcgatc gcgtgagggc acagcgatgc actcttccac ccatgccgcg ctcgcgctgc
     1141 tgctgctggc ggcgcaaggc gcgcatgccc aggcgccgct ccccgcgggc ttcgtcgatg
     1201 ccgccgccgt ggtcgacgga ctggtcgtcg acatgcgcta tttcggcgcc aacaacttcg
     1261 tgggtgaacg aatcgacggc tacgagtgtc cgcgttgcct attatcggcg ccggcggcca
     1321 acgcgctggc ggcggtcgag cgcgatctgg cggcgcgcgg actcggcctc aaagtgttcg
     1381 attgctaccg gccgcagcgc gcggtcgcgc aattcgtgcg ctgggcgcag cggatcgacg
     1441 acgtcaagcg caaacgtgaa ttctacccgg acgtggacaa gcgcgacctg ttcaaggagg
     1501 gctatatttc cgatcgctcc ggccattcgc gcggctcgac cctcgatctc acgctggtgc
     1561 ggcgcgcgga cgcccgcgag ctcgatatgg gctcgccgtt cgacttcttc agcccgaaat
     1621 catggccgtc ggatcgaagc gtgagcgatc aggcgcagca gaaccgcgcg ctgctcgccg
     1681 cggtgatgat ccgcggcggc tttcggccct acgacaggga gtggtggcac ttcacgctca
     1741 acaatgagcc gttcccggat acgtatttcg attttccggt caggtgagct tcgtcagcgc
     1801 gctggcaccg atcaatatgt gaactccgtt tgtaagccat tggatagatt gagaaagatt
     1861 ggcttcgttt ggccgaatac aactaagagc tgtagaacaa aaccgctcgg tctcgccgcg
     1921 cagcagcgcg gcgctcgtat cggtcgagct tgcgcaattc tggcaggacg cgtcggatgg
     1981 cttctgccga tcggtcagtc tcttgtgacg gcatcgtcgc caaggcgtcg atcggtttgg
     2041 ggatcggtct accccgctca aacgcattaa ggacccggat catttgggtg acggtgagct
     2101 gcggcggatc gagctctccg aacgcagacg cgcgttcgat taaggcaacc ttggcccgcc
     2161 gcacccgcgc gaggtcgagc tctgcgtgcg cgatagcgcg ggcgcgctcg agcaagattg
     2221 catcctcgct gtcgccagcg attttgcgga cgagggtgtc cagctgtttc gcaactgccg
     2281 cgctcgaggt gatgctcaag gtcaggccgt ggcggtaggc gttgcggctc gcccgtttct
     2341 tacccgcgcc tgaacgcgga ccggtgctct tgcgcgcgtt gcggcggttg gcggcaattt
     2401 gccgttcgct cgccatcgtc aggccgccgt tgagcgcttg ctggatggat gctcgagtcg
     2461 aagaacatcc ttgccgtcaa tgatccggtc cgcgctctcc
//
LOCUS       DATLJT010000482         7906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2785810,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000482 DATLJT010000000
VERSION     DATLJT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785810"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VM910_11115"
     CDS             <1..362
                     /locus_tag="VM910_11115"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HVI63116.1"
                     /translation="CPYCTLWADGFNGLLPHIENRAAFVVASPDDPQAQEKFKASRGW
                     RFPMVSHRDSTFAADMGYRRDDGWLPGVSVFKKQGGKIVRVADTSFGPGDDFCSIWHF
                     FDLLPEGAADWQPKYKY"
     gene            398..799
                     /locus_tag="VM910_11120"
     CDS             398..799
                     /locus_tag="VM910_11120"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVI63117.1"
                     /translation="MPFEFSVSDFIPASPQDIYDAWLSSSGHEKITGGQKAQISAQEG
                     AAFTVWNGYITGRNLKLEPGRRIVQSWRTTKFTDTDADSQIEVLLQPAAGGTTVTVRH
                     SNVPDGHTSYRDGGWQRSYFEPMKQHFKRAR"
     gene            1167..2135
                     /locus_tag="VM910_11125"
     CDS             1167..2135
                     /locus_tag="VM910_11125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63118.1"
                     /translation="MQRIAVFALTLVLTAVAAVPQANADSYPSRPIRLIIGFPPGSAA
                     DITARLVGDVMSRTLGQQVVVEARPGAGSSIAAEFVAHSAPDGYTLFIGTLSNVTSAV
                     TSSNLRFDFAKDFAPVAPLTGLPLILAVHPSLSLSSVGELIALAKSKPGELTYGSVGP
                     GTVGHLAMELISARTNIKLVHVPYQGSPPQVTDLVAGRLSMALVVASTVMPHVEAGRL
                     RALASGSAKRPRIAPNIPTIAEALLPNFDCTVWFGLMAPAGTPRAVIEKLSAAAQAAL
                     KSEDVVGKLAGSGFEPLDGSPEEFAQLIAHGMATWAAAAEAAGLRK"
     gene            complement(2293..2421)
                     /locus_tag="VM910_11130"
     CDS             complement(2293..2421)
                     /locus_tag="VM910_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63119.1"
                     /translation="MRDIFESEIPDTAPYKEWLGIVGSMLVAVLICAVLFAGGVLT"
     gene            complement(2617..4308)
                     /locus_tag="VM910_11135"
     CDS             complement(2617..4308)
                     /locus_tag="VM910_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVI63120.1"
                     /translation="MNGLRYDRILAGTALALILAIPMGGLLAQDPNKIAPAPTAAAPI
                     EQTAAPAPAPTAAAPVAAAPAEEVATPALAAEPVAVTEQAVPADPLASLDPADRAIAE
                     KMRDLLAATSDKIFASKKERAAAEAFYQSRNFLPLWLDKGVENAHATSAIARLKHADA
                     DGLEVSDYKTPTFAGLGSDALAEAELKLTQAVLTYARHVQAGRFAYTRVSRNIELPQV
                     VPEAADILGTIAAASDAGKALDAFSPQHLEYQKLKAMLAELRATSGGKKSEAPRQVET
                     IIANMERWRWYPRDLGKAHVVVNQPDFTLRVMHEGAQVWTTRIVIGKPSMPTPLLTET
                     MKYITVNPTWHVPPSIVHNEYLPALAQDPTVLDRMGLRVSYSGGSVSIWQPPGPNNAL
                     GRIRFNFPNRFLVYQHDTPDKYYFAHEVRAYSHGCMRVQDPAKYAEVLLNIARPNEHW
                     TAERIKRMFGSGEQDLQLQPTPIWVHLTYQTAFVDNAGKLQTRRDIYNLDSRTLAAIK
                     SARGVIEPTPERRREELVASGPTQRKPAPAVRNGTMSQSMVYPGYARPFPGFFYR"
     gene            complement(4771..5247)
                     /locus_tag="VM910_11140"
     CDS             complement(4771..5247)
                     /locus_tag="VM910_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI63121.1"
                     /translation="MSIPIEISICTDQDYAQILDELHEFWGPRDTRHLHHPFLVHEFG
                     NSAFVIRNGREVVAYLFGFLSQTEPVGYVHTLAVRTSARRRRLAQELFGHFVEFARRH
                     GCKHVKAITTPSNLESIAFHKSLGMQLLGEPNSDGIPVAPDYAGRGAARVVFWKAI"
     gene            5558..6523
                     /locus_tag="VM910_11145"
     CDS             5558..6523
                     /locus_tag="VM910_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVI63122.1"
                     /translation="MADIFISYSKPHVELTRDLASDLEAKGLTVWWDTDLLAGESFRQ
                     RIIQELHDCKAAIVIWTPQSVSSDYVISEAERARKAGKLIQVRTEEVEPDALPPPFDT
                     GHASLIDDRKAIYGALAKLGMLRNESITVSGPLPLFHPERRPAVRKVSTRVLVLAGLA
                     IVAAVGGLASMIWFQEQPASEFHTASDSRDSKVVKQFVDALNARFEDSSVFSSEVRLG
                     RRGNMTRVEAVTDLRRSLSKYSKVNCRTDGSDPTLKSPELGRSGLRLKVYLVCDFGDE
                     TGGTTTEHFPIEIEAAPDAGNKNALQITGLWHSERMVLWQPRARD"
     gene            complement(6659..6735)
                     /locus_tag="VM910_11150"
     tRNA            complement(6659..6735)
                     /locus_tag="VM910_11150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6699..6701),aa:Arg,seq:tcg)
     gene            6962..7363
                     /locus_tag="VM910_11155"
     CDS             6962..7363
                     /locus_tag="VM910_11155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI63123.1"
                     /translation="MAFPDLPAFARLLGVNLLRRSPERTEADLPVRAELCNRRGVLHG
                     GAVMALGDMLGGMTARISLPDGGRTATIESKTNFFAAVPEGDTAHAVCMPLHRGRTTI
                     VLETRITRADGKLAAIVTQTQLIFDVDDANE"
     gene            7490..7765
                     /locus_tag="VM910_11160"
     CDS             7490..7765
                     /locus_tag="VM910_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63124.1"
                     /translation="MTDPRDFDRRMEMERQMEMERRMGAPTIWAWIAGAVFIVVILAL
                     MFAGGESTRTAREDANPPATTGMAPRTAPPAVAVPPAQAPSTTGQSQ"
BASE COUNT         1469 a         2452 c         2527 g         1458 t
ORIGIN      
        1 gttgccctta ctgcacttta tgggccgacg gtttcaacgg cttgctgccg cacatcgaga
       61 atcgcgccgc cttcgtggtc gcgtcgcccg acgatccgca ggcgcaggag aaattcaagg
      121 catcgcgcgg ctggcgcttc cccatggtgt cgcatcgcga ttccaccttc gccgccgaca
      181 tgggctatcg gcgcgacgac ggctggctgc cgggcgtgtc cgtgttcaag aaacagggtg
      241 gaaaaatcgt ccgcgtcgcg gacacgtcgt tcgggccggg tgatgatttc tgctcgatct
      301 ggcacttctt cgatctcctt ccggaaggcg ccgcggactg gcagccgaaa tacaaatact
      361 agtgcggcgg ttcagaagcc cgcatagggg cgaggcaatg ccattcgagt tctcggtatc
      421 cgacttcatt ccggcgagcc cacaagacat ctatgacgcc tggctcagca gcagcggcca
      481 cgaaaagatc accggcgggc aaaaggcgca aatctcggcg caggaaggtg ccgcgttcac
      541 ggtatggaac ggctacatca ccggacggaa tctaaagctt gagccgggcc ggcgtatcgt
      601 ccaatcgtgg cgcaccacca aattcaccga caccgatgca gattcgcaga tcgaggtgct
      661 gctgcagccg gcggcgggcg gcaccacggt cacggtgcgg cacagcaacg tgcccgacgg
      721 tcacaccagc taccgcgacg gcggatggca gcgcagctat ttcgagccga tgaagcaaca
      781 cttcaagcgt gcgcgctgag atcgtgccgg atgcgtggtg gcgcgccacg gtcgatgaag
      841 atgggcacta cgcgtttgcg ccggcactat gatgccgcgg cccgccacgc gccaatcggc
      901 taatctgcga tgcgcggcgg gcttgcacga cgccgcatgg acgagtgtcg tcccgatttc
      961 gccgactggt ccggttacct tcgatagcgg ctcgaccgat cagcccggga accgatgcgg
     1021 tgtgacagca agcgacatcg ccgtcagcct tgcggtcaat taacataaga tcgtttcgcc
     1081 aatctcgcga agcgcacgca aagcggctgg tttggcggca gccggttcgt tggacaacac
     1141 aagccgcgtc caaggggagg actgccatgc aacgcattgc cgttttcgca ctgacgctgg
     1201 tgctgaccgc tgtcgccgcc gtgccgcagg caaacgccga cagctatccg tcgcggccca
     1261 ttcgcctgat cataggcttc ccgccaggtt cggcggccga tatcaccgcg cggctggtcg
     1321 gtgacgtcat gagcaggacg ctgggccagc aagtggtggt cgaagcccgg ccgggcgccg
     1381 gctcgagcat cgccgcggag ttcgtcgcgc actcggcgcc ggacggctac accttgttca
     1441 tcggcacgtt gtcgaacgtc accagtgccg tcaccagctc caatctacgc ttcgacttcg
     1501 ctaaggattt cgctccggtc gcgccactca ccggcctgcc gctcatcctt gcggtgcatc
     1561 cctccctcag cctgtcgagc gtcggtgagc tcatcgcgct cgccaaatca aagcctggtg
     1621 agctcaccta cgggtcggtc gggccgggga cggtcgggca tctcgccatg gaactgatca
     1681 gcgcgcgcac gaacatcaag cttgttcacg tgccctatca ggggagtccg ccgcaggtca
     1741 ccgacttggt cgcgggccgc ctgtcgatgg cattggtggt ggcctcgacg gtcatgccgc
     1801 acgttgaagc cggtcgtctc agggcgctcg cctcgggttc cgccaagcgc ccgcgcatcg
     1861 cgccgaacat cccgaccatc gccgaggcgt tgctgcccaa tttcgactgc accgtttggt
     1921 ttggcctgat ggcaccagcc ggcacgccgc gcgccgtgat cgagaaactt tctgccgctg
     1981 cccaggcagc gctcaagtcc gaggacgtgg tcggcaagct cgcggggtca ggcttcgaac
     2041 cgctcgacgg ctcgcctgag gagttcgcgc aattgattgc gcacggcatg gcgacatggg
     2101 ccgccgccgc tgaggccgcg gggctgcgga aatgagaaga gtcaagcgca agagcggtgg
     2161 cattcggcgc cgatagcgta tcccgaagtg gcgcgccccg agggattcga accccggcct
     2221 ccagattcaa aggctgatct tcacaacact cgtccgcccg atgctgacga gattgcgagt
     2281 gagggggcag cttcaggtta ggacgccgcc cgcgaacagg accgcgcaaa tcaggactgc
     2341 caccagcatg gagcccacga taccgagcca ttccttgtaa ggcgccgtgt cggggatttc
     2401 gctttcaaaa atgtcgcgca tcgcaccacc cgttcgatga accgttatgc catctcccgt
     2461 gcccgcgact gcttgtgtgg aaaaattggc gacaattgtg gagcgtcgga cgcaacgtcg
     2521 aaaaaaccgg gtggtgtagc ttttgggtcg caaagcgatc ggggtccccc cgctctatcg
     2581 cgaggggacc ccgagactag caacgccgaa agacgatcag cgatagaaga agcctgggaa
     2641 cggcctggcg taacccgggt agaccatcga ctgggacatc gttccgttgc gcacggccgg
     2701 agccggcttg cgctgggtcg gtccggacgc caccaactcc tcccgcctgc gctctggcgt
     2761 gggctcgatc acgccgcgcg cgctcttgat cgccgcaagc gtgcggctat cgagattgta
     2821 aatgtcgcgc cgggtctgca gcttgccggc gttgtcgacg aaggcggttt gataggtgag
     2881 gtggacccag atcggcgtcg gctgcagctg caggtcctgc tcgccgctgc cgaacatccg
     2941 cttgatcctc tccgccgtcc agtgctcgtt cggacgggcg atgttgagga gaacttcggc
     3001 gtatttcgcc ggatcctgca cgcgcatgca gccgtggctg taggcgcgaa cctcatgggc
     3061 gaaataatac ttgtccgggg tgtcgtgctg atagaccagg aaccggtttg gaaagttgaa
     3121 ccgaatccgt cccaaggcat tgttgggacc tggcggctgc caaatcgaga cgctaccgcc
     3181 gctgtagctc acgcgcagtc ccatgcgatc gagcacggtc ggatcctgtg ccagcgccgg
     3241 cagatattcg ttgtgcacga ttgacggcgg cacgtgccac gtcggattga ccgtgatgta
     3301 tttcatcgtt tcggtcagca gcggcgtcgg catgctgggc ttgccgatga caattcgggt
     3361 ggtccagacc tgggcaccct catgcatcac ccggagcgtg aagtccggct ggttgacgac
     3421 cacgtgggcc ttgccgagat cgcgcggata ccagcgccag cgttccatgt tggcgatgat
     3481 ggtctcgacc tgccggggag cttcgctttt tttgccgccc gaggtggcgc gcagctccgc
     3541 cagcatcgcc ttgagcttct gatactccag atgctggggg ctgaacgcgt cgagcgcctt
     3601 gcccgcgtcc gacgcggcgg cgatcgtacc gaggatgtcc gcggcctcgg gcaccacttg
     3661 cggaagctcg atgttacggc tgacgcgggt gtaagcgaag cgcccagcct gcacgtggcg
     3721 ggcataggtg agcaccgcct gcgtgagctt gagctcggct tcggccagcg catcggatcc
     3781 gaggccggca aaagtcggcg tcttataatc gctcacctcg agcccatcgg catcggcgtg
     3841 cttcaaacga gcgatggcgg acgtggcgtg tgcgttctcg acgcccttgt cgagccagag
     3901 cggcaggaag ttgcggctct ggtagaacgc ctcggctgcc gcgcgctctt tcttgctggc
     3961 aaaaattttg tccgaggtcg cggccaggag gtcgcgcatc ttttccgcaa tcgcgcggtc
     4021 ggcgggatcg agtgacgcga gcggatcggc cgggacggcc tgctctgtca cggccaccgg
     4081 ttcggccgca agcgctgggg ttgcaacctc ctcggcaggt gcggctgcga cgggggcggc
     4141 cgcggtgggc gcgggcgccg gggccgcggt ctgctcgatg ggcgcggctg cggtaggcgc
     4201 gggcgcgatt ttgttgggat cctgcgccag caggccgccc atcgggatgg caagtatcag
     4261 cgctagcgcc gtccctgcca ggatacgatc ataacgaagg ccattcatga gatattctcc
     4321 ccaagaggtg aacttgtcgt ggaaggtcct tggcccgcgc gcgcaggcgc gatccaggtg
     4381 agccggacgc agcaaaaatc gttgaatgcc agcatcgcca ggcccaattc gccgtcggcg
     4441 agccgaattc gtcgtcagag ccgcgtttgt atgtgcaagg cccccctccc cggttaccta
     4501 tcgaccgcca ccccactgcc ggctcggtcg ccggcgatgg ctgtcgacct attggtcgcg
     4561 ctggggggtc aattggttca aacgcgatgg ggggatcagg aggttggcgc cggggcgtta
     4621 accaaggtta acgaaggggg ccgaccctcc ggggccgaag gcccgtgccg ccaagccagt
     4681 ctcggacgga gccccggacc tcggcgcgat gacctacagg cgaggtgcag ccgatccgcc
     4741 cgtatccgcc cgtggcgtaa cggtgtccgc ctagatcgcc ttccaaaaga ctacgcgcgc
     4801 tgcgccgcgg ccggcatagt cgggggcgac gggaattcca tcgctattgg gttcgccgag
     4861 gagctgcatg ccgaggcttt tgtgaaatgc gatggactcc agatttgacg gcgtggtgat
     4921 cgccttgacg tgcttgcaac catgccgccg agcgaactcg acgaagtggc cgaagagctc
     4981 ctgcgcgagg cgtcgcctgc gggcagacgt gcggacggcg agcgtatgca cgtacccaac
     5041 cggttcggtc tgcgagagaa agccaaacaa atacgcgacg acctcccttc cgttgcgaat
     5101 gacgaacgcc gagtttccaa actcgtgaac aaggaatgga tggtgcaggt ggcgggtatc
     5161 ccgcggaccc cagaattcat gcagctcatc gagaatctgg gcgtagtcct ggtcggtaca
     5221 gatcgaaatt tctatgggaa tgctcatcgc cgtcccgctg gcatggtggc ccgcaagaat
     5281 cttttgtggc agtgggtcaa acgaggcggc aggttagcgt tgagagcaga tcactgcacg
     5341 cagagaaaca gcggaaagac ggactgccgc caagcagatt ttcaacgggt ttcgttatca
     5401 agacgtctcg cctttcatgc tcggtgtgaa gccgatcaca cgacttttgg aaaaagttgc
     5461 gagaatttaa actgtctgtt ttacgagtac ggcaacgcca tgtgacttga agcgccggaa
     5521 tggttgacgt ccaatcatct tggttggagc gcatctgatg gcagacatat tcatcagtta
     5581 ctcgaagccg catgttgagc tcacgcggga tcttgcctcg gatcttgagg caaaaggcct
     5641 caccgtctgg tgggacaccg accttcttgc cggggaatcg ttccgtcagc gcattattca
     5701 agagctccac gattgcaagg cggcgatcgt tatctggaca ccgcagtctg taagctcgga
     5761 ttacgtgata tccgaagccg agcgtgcgcg caaagcgggc aagttgatcc aagtccgaac
     5821 tgaggaggtt gagcccgacg cattgccgcc accgttcgac accggccacg catcactgat
     5881 cgacgaccgc aaggcgatct acggtgcctt ggccaagctt ggtatgcttc gcaacgagag
     5941 cataaccgtg tccgggccgc tgcccctgtt ccatccggaa aggcggccgg ctgtgcggaa
     6001 ggtgtcgacg agagtgctcg tcttggcggg gctcgcgatc gtcgccgcgg ttggtgggct
     6061 ggcgagtatg atctggttcc aggaacagcc tgcctcggag tttcacacgg cttccgacag
     6121 tcgcgattcc aaggtcgtca agcaattcgt cgacgcgctg aacgctcgct ttgaggattc
     6181 aagtgtgttc agttcagagg ttagactcgg ccggcggggg aatatgacca gggtcgaggc
     6241 ggtgaccgac cttcgccgat ccttgtccaa atatagcaaa gttaattgcc gtacggatgg
     6301 aagtgatcca accctgaaat ctcccgagct cggacgcagt ggattgcgtc tcaaggtcta
     6361 tctcgtgtgc gatttcgggg atgaaaccgg cggcaccacg accgagcatt tccccattga
     6421 aatcgaagcg gccccggatg cgggtaacaa aaatgcgttg cagataacgg gattgtggca
     6481 ctcggaaaga atggtgctgt ggcagccgag ggcgcgcgat tgacggcact aaattcgatc
     6541 gcgaaatcga atctgtcacc tcccgcaagc ctcatgtcga ttgccagagc ccgacccatc
     6601 tggagcgcac cagatgtctg acgacgacga ttgaatgacc agatcgcgca gatttgtttg
     6661 gcgcgctcgg agggattcga acccccgacc cccagattcg aagtctggtg ctctatccag
     6721 ctgagctacg agcgccgcag ccctttctaa cgaagagcgc cgctgaattc cacttgcgct
     6781 ttgctcgacc acaagcgcgt catgtcgtga tctcgcggca agccgcgcgc gacgaaaggc
     6841 atccgcccgc cccgaccgag cgcatcgatc cgcgcgtttg cagacatccc cgatcgcgca
     6901 tccggctggc actgggcgtg ggccatgcta tgagcacatc ggccgcagca gaggaacaac
     6961 catggccttc cccgatctcc ccgcattcgc caggctcctc ggcgtgaatc tcctccgtcg
     7021 ctcgcccgaa cgcaccgaag ccgatctgcc cgtgcgcgcg gagctgtgca accgccgcgg
     7081 cgtgctccat ggcggcgcgg tgatggcctt gggcgatatg ctgggcggga tgacggcgag
     7141 gatcagcctt cccgacggcg gccgcaccgc gaccatcgag agcaagacca atttttttgc
     7201 cgccgtgccg gagggcgaca ccgcgcacgc cgtctgcatg ccgctgcacc gcggccgcac
     7261 caccatcgtg ctggagacgc gcatcacccg cgccgacggc aagctcgccg ccatcgtgac
     7321 gcagacccag ctgatcttcg acgtagacga tgccaacgag tgacggcggc cgttcgtggt
     7381 cggcgttcag aaccatcacc tgaaggcggc cctaaagggt tccatgggcg gaaccaggcg
     7441 cctccccgcg gggttgtcta tgcacggaca tcagcgatgg gagatcgcca tgacggaccc
     7501 gagagacttc gatcgcagaa tggagatgga gcgccagatg gagatggagc gccggatggg
     7561 cgctcccacc atatgggcat ggatcgcagg cgccgtgttc atcgttgtta ttctggcgct
     7621 catgttcgcc ggcggcgaga gcacgcggac cgcacgcgaa gacgcaaacc cgcctgccac
     7681 caccggcatg gcgccgcgaa ccgcgccgcc cgctgtcgcc gtccctccgg cgcaggcgcc
     7741 ctcaacaacc ggtcagagcc aataacggcg cgcacacacc catggagatt tttcgttgac
     7801 tgcagcaccc cggctcccga agccggggtg ctcgcttttt cggcgcgagc ttcagcgcgt
     7861 ccgccattca cggcaggtcg aacatgatca attgatccgt tatccc
//
LOCUS       DATLJT010000483         7032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2325548,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000483 DATLJT010000000
VERSION     DATLJT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325548"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /gene="guaB"
                     /locus_tag="VM910_11165"
     CDS             <1..1490
                     /gene="guaB"
                     /locus_tag="VM910_11165"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HVI63125.1"
                     /translation="AQEFQGFREAFTFDDVLLKPGLSDVLPSEVDIRSRVTRAIPLNI
                     PIIASAMDTVTEARMAIAMAQSGGVGVIHRNFDPEAQAAQVRQVKKYESGMVVNPLTI
                     GPGAMLSDALTLMKDHGFSGIPVVVGSGNGKPGKLVGILTNRDVRFATDPRQKVSELM
                     THENLVTVREGVSQDEAKRLLHKFRIEKLLVVDEQYRCVGLITVKDMEKAVTHPLACK
                     DGQGRLRVAAATTVGEGGYERTERLIDAGVDLIVVDTAHGHSSRVLEAVNRIKQLSNE
                     VQVIAGNIATREGAQALIDAGADAIKVGIGPGSICTTRIVAGVGVPQLTAIMDAVEAA
                     KKADVPVIADGGIKFSGDLAKALAAGADIAMVGSLLAGTDETPGEVFLWQGRSYKAYR
                     GMGSVGAMARGSADRYFQQDIKDTLKLVPEGIEGQVPYKGPVANVMHQLAGGLRAAMG
                     YVGARNIAEFHEKAQFVRITGAGLRESHVHDVTITREAPNYPGGI"
     gene            1597..2619
                     /locus_tag="VM910_11170"
     CDS             1597..2619
                     /locus_tag="VM910_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVI63126.1"
                     /translation="MPQAKRIVLASRPVGEPKPSDFRIEEIPVPAPGPGQVLLRTIWL
                     SLDPYMRGRISDAASYAQPVPIGGVIEAGTVCEVIASNNPAFAKGDIVLARAGWQTHA
                     LSDGKGLAKIDPKLAPISTAVGVLGMPGMTAYTGLLDIGRPQPGETVVVAAASGAVGS
                     AVGQIARIKGARAVGIAGGKDKCEFVKNELGFDHCVDHRDPDLAARLKDACPKGIDVY
                     FENVGGAVFDAVFPLLNPFARVPVCGLIAHYNDTEAKPPKWAASLMRAILTKRLTFRG
                     FIVSDFAARHADFLRDMSQWVREGKVKYREFVTEGLDTAPAAFMGLLKGANFGKQLVR
                     VGPDRE"
     gene            complement(2701..3558)
                     /locus_tag="VM910_11175"
     CDS             complement(2701..3558)
                     /locus_tag="VM910_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI63127.1"
                     /translation="MTEFRLTQISDPHLARRLTKLTANFDAVSDYIDATRPDLVVNTG
                     DLAFDAPTNLDDLEFARTLHDALPVACRYLPGNHDVGDNPTQIGPAPTHPVTERRRQA
                     FIGAFGDDRWRFEAAGWCFIGLNSLVMNSGLVSEAEQFEWLASELASVHGKPVALFLH
                     KPVYLNAPDDPELEASAIRYVPMPARRRLVEMLRAVDLRLIASGHVHQRRDFTYRHIR
                     HVWAPSAGFIISDKRQERIGMKEVGFVEYRFQPDSFEVRHVRAPGQINVDLDELLAKP
                     PAAEPARAN"
     gene            complement(3659..4249)
                     /locus_tag="VM910_11180"
     CDS             complement(3659..4249)
                     /locus_tag="VM910_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acriflavin resistance protein"
                     /protein_id="HVI63128.1"
                     /translation="MEFSSPFLMSALSRAAAALAVAIALTVYFHSGYRTRRDIIRDGF
                     ATVVALGLFAFVAYDMRHAAMAYLGINTSKAAVEFEIRLPRSAWSTITETQVELHTDR
                     NQTLARVEGSNDSNDGRSVLRGVVPLDYRTTDRIVVLNLPGNAQCEFKLRLAADPSRS
                     DKFGPWHLADRVAIPSASETTDAEPRHEFAIRYRVL"
     gene            4493..5791
                     /locus_tag="VM910_11185"
     CDS             4493..5791
                     /locus_tag="VM910_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HVI63129.1"
                     /translation="MTPAARLSAAIEVLETIETQRIPAAQALKEWGTSHRFAGSGDRA
                     AIAGLVWDVLRRRASSGWIMDADTPRARILGMLKLERGMDMPAIASLCDGNRFAPHPL
                     TEAERVALTSRSLEDAPDPVAGDYPEWLDPYLATVFGDERVAEAAAMASRAPLDLRVN
                     TLKAKREKVLASLAHLDAVPTPWSPFGLRIRLSADARNVGIHGEEAFIKGFVEVQDEG
                     SQLAALFSAAKPGEQVVDLCAGAGGKTLALAAMMQGRGRLIATDGDKRQLAPIHARLA
                     RAGVHNAEVLTPKGAADPLADIKASADLVVIDAPCSGTGTWRRNPDAKWRMRPGALEI
                     RLKDQVEVLDRAAAMVKPGGRIAYITCSVLKAENSDQVRAFAARHDEFSIAPPAETVA
                     VLGDKADDFAKAALQAPEGWLMTPRRTGTDGFFVSLLRRA"
     gene            5945..>7032
                     /gene="guaA"
                     /locus_tag="VM910_11190"
     CDS             5945..>7032
                     /gene="guaA"
                     /locus_tag="VM910_11190"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HVI63130.1"
                     /translation="MTAAQQKARESTPSVASAHDKILIVDFGSQVTQLIARRVREEGV
                     YCEIVPFQKAEAAFRDMKPKAVILSGGPESVHVEGSPRAPQLIFDSGVPVLGICYGQM
                     TMAAQLGGDVEGGHHREFGRADVEVKTDSCLFDATWRPGERHPVWMSHGDRITRMPPG
                     FVVAGVSANAPFAVIQDEKRKYYGLMFHPEVVHTPDGAKLIRNFVRKVAGLTGDWTMR
                     AFRDEAIEKIRAQVGRGRVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNE
                     AETVVDLFRHHYNIPLVHVDASRQFLAELAHVTDPEKKRKTIGRLFIDVFEAEARQIG
                     GAEFLAQGTLYPDVIESVSFTGGPSVTI"
BASE COUNT         1269 a         2117 c         2330 g         1316 t
ORIGIN      
        1 tggcgcagga atttcaaggc tttcgcgaag ccttcacctt cgacgacgtg ctgctcaagc
       61 ccggtttgtc ggatgtcctg ccgtccgagg tcgacattcg ctcccgtgtc acccgcgcga
      121 ttccgctcaa catcccgatc atcgcctccg ccatggacac tgtcaccgag gcgcgcatgg
      181 ccattgccat ggcacaatcg ggcggcgtcg gcgtcatcca ccgcaatttt gacccggagg
      241 cgcaggcggc ccaagtgcgg caggtgaaga agtacgagtc cggcatggtg gtcaatccgc
      301 tgaccatcgg cccgggtgcc atgctctcgg acgcgctcac gttgatgaag gaccacggct
      361 tctccggcat tcccgtcgtc gtcggcagcg gcaacggcaa gcccggcaaa ctggtcggca
      421 tcttgaccaa ccgcgacgtg cgctttgcca cggatccgcg tcaaaaggtg tccgaactga
      481 tgacgcatga aaaccttgtc acggtgcgtg agggtgtcag ccaggacgaa gccaagcgct
      541 tgcttcacaa gtttcgcatc gaaaagctgc ttgtggtcga tgagcaatat cgctgcgtcg
      601 ggctgatcac cgtgaaagat atggaaaagg cagtgacgca tcccctcgcc tgcaaggatg
      661 ggcaggggcg gttgcgcgtc gcggctgcaa ccacggttgg cgagggcggc tatgagcgta
      721 ccgaacggct gatcgacgct ggtgtcgatt tgatcgtggt cgacacggcc catggtcatt
      781 cctcgcgcgt cctggaggcg gtcaaccgca tcaagcagtt gtccaatgag gtgcaggtga
      841 tcgcgggcaa tatcgcgaca agggagggag cacaggcact gatcgatgcc ggtgctgatg
      901 ccatcaaggt cggcatcggc ccgggctcga tctgcaccac gcgcatcgtc gcaggtgtcg
      961 gcgttccgca gctcacggcg ataatggatg ctgtcgaggc tgcgaagaag gccgatgtac
     1021 ccgtgatcgc cgatggcggc atcaaatttt cgggcgatct ggcgaaagca ctcgccgccg
     1081 gtgccgacat cgcgatggtt ggctcgctgc tcgccggcac ggacgaaacg cccggtgagg
     1141 tctttctgtg gcagggacgc tcctataagg cctatcgcgg catgggttcg gttggcgcga
     1201 tggcgcgcgg ctcggcagac cgttatttcc agcaggatat caaggacacg ttgaaactgg
     1261 tgccggaggg catcgaaggc caagtgccgt acaagggacc ggtcgccaat gtcatgcatc
     1321 agctcgccgg cggcttgcgc gccgccatgg gctatgtcgg agcccggaat atcgccgaat
     1381 tccacgagaa ggcgcagttc gtgcgcatca ccggtgcggg tctgcgcgag agccacgtcc
     1441 atgacgtgac gattacacgc gaagcgccga actatcccgg tgggatttag ccgtcattgc
     1501 gaggagcgta agcgacgagg caattcagtc ttttcgattg accaagctgg attgcttcgc
     1561 ttggaatgac gaaagacaga actagaggaa actgccatgc cccaagccaa gcgcatcgtt
     1621 ctggcgtcgc gtcccgtcgg cgagcccaaa ccatccgact tccgcatcga agaaattcca
     1681 gtgcctgcgc ccggacccgg gcaggtgttg ttgcgcacga tctggctttc gctcgatccc
     1741 tatatgcgcg gccgcatcag cgatgcagct tcctatgccc agccggttcc aatcggcggc
     1801 gtgatcgaag ccggcaccgt gtgcgaggtg atcgcttcca acaatcccgc ctttgccaaa
     1861 ggtgacatcg tgctggcgcg cgccggctgg cagacgcatg cgctgtccga cggcaagggc
     1921 ctcgccaaga tagatcccaa gcttgcgccg atctcgacgg cggtcggcgt gctgggtatg
     1981 cctggcatga ccgcgtatac ggggttgctc gacatcggca ggccacagcc gggcgagacc
     2041 gtcgtcgtgg cggcagcctc cggcgccgtg ggctcggctg tcggtcagat tgcgaggatc
     2101 aagggtgcac gcgcggtcgg cattgccggc ggcaaggata agtgtgagtt cgtcaagaac
     2161 gagttgggct ttgaccactg cgtcgatcac cgtgatcccg accttgcggc gaggttgaaa
     2221 gatgcctgcc caaagggcat cgacgtctat ttcgagaatg tcggcggtgc agtgttcgat
     2281 gcggtgtttc cgctgttgaa cccgttcgcg cgagtgccgg tctgcgggct gatcgcgcac
     2341 tataacgaca ccgaggcgaa accgccgaaa tgggcggcat cgctgatgcg tgcaatcctc
     2401 accaagcgat tgacgttccg cggcttcatt gtgagcgatt ttgccgcacg gcatgccgac
     2461 ttcctccgtg acatgtcgca gtgggtgcgc gagggcaagg tcaaatacag ggagtttgtc
     2521 accgaaggcc tcgacaccgc accggccgcc ttcatgggcc tcttgaaggg cgccaatttc
     2581 ggcaagcagc tcgtgcgcgt tggtcctgac cgagagtagg attgaactgc ccgagagtac
     2641 cgaggcgcgg agcaggctgc ttgccactat caagcaggaa ttgaagaacg cctcgcaact
     2701 ctagttcgcc cgtgcgggct ccgcggccgg cggcttcgcc agcaactcgt ccaaatcgac
     2761 attgatctga ccgggcgccc tgacgtgacg tacctcgaag ctgtcgggct ggaatcggta
     2821 ctccacgaag cccacctcct tcatgccaat gcgctcctgc cgcttgtccg aaatgatgaa
     2881 tccggcggac ggcgcccaga catggcggat gtggcgatag gtgaaatcgc ggcgctggtg
     2941 gacgtggccg ctggcgatta gtctgagatc gaccgcacgc aacatctcga cgaggcggcg
     3001 ccgcgccggc atcggaacgt agcggatcgc gcttgcttcc aattcgggat cgtcgggcgc
     3061 gttcaggtaa accggtttgt gcaggaacag tgcgacgggt ttgccatgga cgcttgcaag
     3121 ttcggacgcg agccactcga actgctcggc ttcgctgaca agacccgaat tcatcactag
     3181 ggaattgagg ccgataaagc accagcccgc ggcctcaaac cgccatcggt cgtcgccgaa
     3241 tgccccgatg aaggcctgcc ggcgtctttc cgtcacagga tgcgtggggg cggggccgat
     3301 ttgggtcgga ttgtcgccga cgtcgtggtt gcccggcaag tagcggcagg caaccggcag
     3361 agcatcgtgc aaggttctcg caaactccag atcgtccagg ttcgtcggag cgtcgaacgc
     3421 aaggtcgcct gtgttgacga cgaggtcggg gcgcgtggcg tcgatataat cgctcacggc
     3481 gtcgaaattc gcggtcagct tggtcaggcg ccgcgcgaga tgggggtcgg agatttgggt
     3541 caggcgaaat tcggtcatgc aaccttcctt tcagccccga ttttagccgg aggccgcttc
     3601 ggaatcatga cgaacattta cgcttatcct tccggtgaac gccgcatccg gggaaatctc
     3661 agagtacgcg atagcggatc gcgaactcgt gacgtggctc ggcgtcggtg gtctcgctcg
     3721 cggaggggat ggccacccgg tcggcgagat gccagggtcc gaacttatcg gagcggctgg
     3781 gatccgcggc aaggcgcagc ttgaattcgc actgggcgtt gccgggcagg ttgagcacga
     3841 cgatgcggtc ggtggtgcgg tagtcgagcg gcacgacgcc tctcagcacg ctgcggccgt
     3901 cattggagtc gtttgacccc tccacccgcg ccagggtctg gttccggtcg gtgtggagtt
     3961 ccacctgggt ttcggtgatg gtcgaccacg ccgatctcgg cagacgaatt tcgaattcga
     4021 ccgcagcctt ggaggtgttg atgccgagat aggccatcgc cgcatggcgc atgtcgtaag
     4081 cgacaaaagc gaacagcccg agggcgacga ccgtggcaaa cccgtccctg atgatgtcgc
     4141 gcctggtgcg gtagccgctg tggaaataga cggtcagtgc gatcgcgacc gcaagtgccg
     4201 ccgcggcgcg tgagagcgcg gacatgagaa acggggacga aaactccata agcccgggct
     4261 tccgagtgcc gcttgagcca aaatgactgc aaccatcgac agttggtctg gtctgccgcg
     4321 aggtcggttc aagtgcggga ggtcgtttta ttgccagggc catccgggtt ccggtaagag
     4381 gggcgtccgc tgcgcgagcg gaggagaatt gcgatgtcgg tccagatggt tttgctgcag
     4441 cttgtggtgc aggtcggggt ctctttcttc cccgcgactt cgcgagtttg acatgacccc
     4501 cgcagcgagg ctctccgccg ccatcgaagt gctggagacg atcgaaacgc aacgcattcc
     4561 ggcggcccag gcgctgaagg agtggggcac gtcccatcgc ttcgccggct ccggcgatcg
     4621 cgcagcgatt gcaggactgg tctgggatgt gctgcggcga cgcgcgtcga gcgggtggat
     4681 catggatgcc gatacgccgc gtgcgcgcat ccttggcatg ctgaaactcg agcgcggcat
     4741 ggatatgccg gccatcgcaa gcttgtgcga cggcaaccgt ttcgcgcctc atccattgac
     4801 cgaggccgag cgcgtcgcgc tgacctcgcg ttcgcttgag gacgcgcctg accccgtcgc
     4861 cggtgattat cccgaatggc tcgacccgta tcttgcgacc gtcttcggcg acgaacgcgt
     4921 cgctgaagct gctgcgatgg caagtcgcgc gccgcttgat ctgcgcgtca acacgctgaa
     4981 agccaaacgc gagaaggtgc tcgcttccct ggcccatctc gatgctgtgc cgacgccgtg
     5041 gtcgcccttc ggcctgcgca tcaggctctc cgccgatgca cgcaatgtgg gtatccatgg
     5101 ggaggaggct ttcatcaagg gctttgtcga agtgcaggat gaaggctccc agcttgcggc
     5161 gctgttttcg gcggccaagc ccggcgagca ggtggtcgac ctctgcgccg gtgccggcgg
     5221 caaaacgctg gcgctggcgg cgatgatgca gggcaggggc cgcctgatcg caaccgacgg
     5281 cgataagcgc cagcttgcgc cgatccatgc gcggctcgcg cgtgccggag tccacaacgc
     5341 cgaggtgctg acgccgaaag gagccgccga tccgctggcc gacatcaagg cgagtgccga
     5401 ccttgtcgtg atcgacgcgc cgtgttcggg caccggcacc tggcgccgca atcccgatgc
     5461 caaatggcgc atgcgtcccg gcgcgctcga gatccgcctg aaggatcagg tcgaagtgct
     5521 tgatcgtgcg gccgccatgg tgaagccggg cggacggatc gcctatatca cctgctctgt
     5581 gcttaaggcc gaaaactccg atcaggtccg cgcctttgcc gcccgccacg acgaattttc
     5641 gatcgcgccg ccagccgaga cggtggccgt gctgggggat aaggcggacg atttcgccaa
     5701 ggccgcgctg caggctcccg agggctggct gatgacgccg cggcgtaccg gcaccgatgg
     5761 cttcttcgtt tccctgctgc gccgcgccta gagcgcgagg tggtttgcgt gatgccggtt
     5821 cggcagggct ttccatatat gtcaataata ttgacatata cggatttgtc catgcctgca
     5881 gaagcatgtg ggccgggcca ctgcgcgggt tgcgggagcg gcttgcgtcg cgtacttcct
     5941 caccatgaca gccgcacagc agaaagctcg ggagtcgacg ccatcggtgg cctcggcgca
     6001 tgacaagatt ctgattgtcg atttcggcag ccaggtgacc caattgatcg cgcggcgcgt
     6061 gcgcgaggag ggggtctact gcgagatcgt gccgtttcag aaagccgaag ccgccttccg
     6121 cgacatgaag ccgaaggcgg tgatcctctc gggcgggccg gagtcggttc acgtcgaggg
     6181 ctccccacgc gcgccgcaac tcatcttcga ttccggcgtg ccggtgctcg ggatctgcta
     6241 cgggcagatg accatggcgg cccagctcgg cggcgacgtc gagggcggcc accatcgcga
     6301 attcggccgc gccgacgtcg aagtcaaaac cgatagctgc ctgttcgatg cgacctggag
     6361 gcccggcgag cggcatccgg tgtggatgag ccatggcgac cgcatcacca ggatgccgcc
     6421 gggctttgtt gtcgccggcg tctcggccaa tgcacccttc gcggtgatcc aggacgagaa
     6481 gcgcaagtat tacggcctga tgttccaccc cgaggtcgtt catacgccgg acggggcaaa
     6541 actgatccgc aattttgtcc gcaaggtcgc gggcctcacc ggcgactgga cgatgcgggc
     6601 cttccgcgac gaggcgatcg aaaagatccg cgcgcaggtc ggccggggac gggtgatttg
     6661 cggcctttcc gggggcgtgg attccgcggt cgcggccgtg ctgatccatg aagcgatcgg
     6721 cgaccagctt acctgcgtgt tcgtcgacca cggcctgttg cgcttgaacg aagccgagac
     6781 ggtggtcgat ctgttccggc atcactacaa cattccgctc gtacacgtcg atgcctccag
     6841 gcagtttctg gccgagcttg cccacgtcac cgaccccgaa aaaaagcgca agacgatcgg
     6901 gcgcctcttc atcgatgtgt tcgaggccga agccaggcag atcggcggcg cggagttttt
     6961 ggcgcagggc acgctctacc ccgatgtgat cgagagcgtg tcgttcaccg ggggaccgtc
     7021 cgtcaccatc aa
//
LOCUS       DATLJT010000484         1642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2785874,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000484 DATLJT010000000
VERSION     DATLJT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785874"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1044)
                     /locus_tag="VM910_11195"
     CDS             complement(100..1044)
                     /locus_tag="VM910_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63131.1"
                     /translation="MPKSWRDVLPIHPAAELFPRMSDDELRALGEDIKKNGLQQAVVL
                     LRENGCECLLDGIGRLDAFEAVGVNLVKNGKLNLSNVRLIIDDIDPYAFVISANIHRR
                     HLTAEQRRALIAKLLKATPEKSDRQIAETVKASPTTVGSVRAEMESTVQVGQLDKRVG
                     KDGKARKQPSRKTSERKSREEKAAYEAGKLLGALEIASEAVQRDFIQNLPAEMLAERD
                     DIGSASTGEAARKDAEIEELRNAKRRLEIENTGLRSEVEELRTANAALRKSLEEASAK
                     FVELSGQFDELRHRMGQAAPPPADDGLDIPASLRRSAQ"
BASE COUNT          251 a          525 c          493 g          373 t
ORIGIN      
        1 cggctctcgt ggtcgtgtac attgcctcga tcagatcgag ggaccgccgc gccatgccgc
       61 gacctttttt ggaactggtt tcaaaatact cggcggcggt cattgcgcgc tccgccgcag
      121 gctcgctgga atgtcgagcc cgtcatcggc gggcggcggc gcggcctgac ccatccggtg
      181 gcgcagttcg tcgaattgcc cgctcagctc gacaaacttt gcgctggctt cttcgagcga
      241 cttgcgcagc gcggcgttcg cagtgcgtag ctcctcgact tcgctgcgca gcccggtgtt
      301 ttcgatctca agccgtcgct tcgcgttgcg cagttcttca atctcggcgt ccttgcgcgc
      361 ggcttcgccc gtgctcgccg agccgatgtc gtcgcgctca gcgagcatct cagccggtaa
      421 gttttggatg aaatcgcgct gcacggcctc gctagcgatc tccaaggcac cgagcagctt
      481 gccagcctcg tacgctgctt tttcttcgcg agatttgcgc tcgcttgttt tgcgcgacgg
      541 ctgcttgcgt gctttgccgt ccttgccgac tcgcttgtcc aactgtccaa cttggacagt
      601 tgattccatc tcggcgcgca cgctgccgac cgtggtcgga ctcgccttca ccgtctctgc
      661 gatctgccgg tcggatttct cgggcgtcgc cttgagcagc ttggcgatca gcgcgcgccg
      721 ctgctcggcg gtgagatgac ggcggtgaat gttggcggaa atcacgaagg catacggatc
      781 gatgtcatcg atgatgagac ggacgttgct gagattaagc ttgccgttct taaccaagtt
      841 gacgccgacg gcttcgaacg catcgagcct gccgatacca tcgagcaagc actcgcatcc
      901 gttctcccgg agcagcacga ccgcctgctg cagtccattt ttcttgatgt cctcgccgag
      961 cgcccgcagt tcgtcgtccg acatgcgcgg gaaaagctcg gcggccggat ggatcgggag
     1021 aacgtcgcgc caggattttg gcattgtgca gccctccggt ttgctccagg gccggggctg
     1081 cgctacaaca cctttgtccg cccggacctc ggtgtcggtg cgcgttttca ccggctcatc
     1141 aaatgcaaac ggcggccctc acgggtcgcc gtttttgcct cagacagttt cggccttgcg
     1201 ctcgcggtct cggggtggtg ccccttcgag gcggcgctcg atcgcgcgtt gacagcgcgg
     1261 agtaacgatt aagagccgtc cgacccgatc tactggaatg tcgccgcgct gggcggcggc
     1321 gtagctgccg ttccgcgaca gcccccaata cttcttgccc gctgcgggga cagataacga
     1381 attaggtcgc ttgcgcattt tggtcgacct cctaacccca tcgatggggt catcctattt
     1441 catcccggtt ttggaggcaa ccccacttgt ggggtcatcc tgttttgtgc tatctcgtgt
     1501 tgatcgctcg gggacgaacc atgccgcgca aacaatccgc catcctcgac tacaagctcc
     1561 gcatccgcga aagcttgcgc cgccggattg aggtcgccgc caagaggcgc aacgtatcgc
     1621 tgaacttcga gatggcgagt cg
//
LOCUS       DATLJT010000485         7076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3245308,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000485 DATLJT010000000
VERSION     DATLJT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3245308"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..802
                     /locus_tag="VM910_11200"
     CDS             326..802
                     /locus_tag="VM910_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63132.1"
                     /translation="MSREGKYAAWFRTPRGEGTGVVHIAEGKIRGGDSTFDYAGSYEI
                     DEDQFTATLMTRRYGSGPTTLFGFDEVVVKLKGFFKGRTVFCSGVAEQAPDIHFEATL
                     FPSHDEVRAVEVKREAATFDAAKLPKSEGNRRRARNPFGTGRPIDRMCTAGPPRRS"
     gene            935..1228
                     /locus_tag="VM910_11205"
     CDS             935..1228
                     /locus_tag="VM910_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI63133.1"
                     /translation="MYAAIRQAKAKTGKAEELATRIKEGAIPIISDVEGFMAYYVVYA
                     PDDTVTAISVFNNFASAEEANRRALAWAEENLAPLLIGPATAVAGPVIVHTLA"
     gene            complement(1380..2216)
                     /locus_tag="VM910_11210"
     CDS             complement(1380..2216)
                     /locus_tag="VM910_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63134.1"
                     /translation="MRAASLIPVMIAITLSATPAGAGSLVIKVDAPKEASQPTTEADF
                     EKYRGYMYDLSEYAGRKDAAAITDNLKHQLDMVENAGFSPRVLEFFHTIPIVASETDC
                     LELGAGIACYGPVAPTRRRHASLGLTVWDHDRQQWSNPDVVDLAADSGRGVIMLRPNM
                     MQYTDDPILLHEFLHAYHGRLMSMGFDNRGIKAFYAEAKAKGLFGKNAYTMLNDREFF
                     AVTASIFLAGKESIHEPKTRENLKEKMSEYYKYLVGVFGFDPDPSLKSPSSGTPVASA
                     AN"
     gene            complement(2368..3204)
                     /locus_tag="VM910_11215"
     CDS             complement(2368..3204)
                     /locus_tag="VM910_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HVI63135.1"
                     /translation="MMTLPELAADALEEFLSSFMRRRFGVAEAAYVEAVPNAARLAME
                     CIGNSDALYHNIEHTLLVTLAGHDIMRGRAQHTHVIAADYAHVIVACLTHDIGYVRGA
                     LKGDNEGGFVVDQSGSKVNLPRGSSDASLMPYHVDRSKLCVLQRLPDSFLIDKQRVAS
                     AIEGTRFPPNEGQSYGVEASIVRAADFIGQLGDPNYIRKANALYHEFEEVGMNRQLGY
                     RSPADIVDRYPQFYWESVSPHIQNAIGDLNRTSSGRQWIANLYANVFRAEQDISLSGP
                     QT"
     gene            3579..3845
                     /locus_tag="VM910_11220"
     CDS             3579..3845
                     /locus_tag="VM910_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVI63136.1"
                     /translation="MTVQERKKVQREPRRQLNGRTAQMTVDGTTEFECSVLDVGPGGV
                     KIMTEAEIDVGARFALALVPTHPRRQQCEVVWRRGNTFGVKFVA"
     gene            complement(3858..6035)
                     /locus_tag="VM910_11225"
     CDS             complement(3858..6035)
                     /locus_tag="VM910_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVI63137.1"
                     /translation="MSQLFRPLPCLFVLAALAGTSTVALAARHKEPAHEKKALAASSG
                     KQERKASLKKEEKKGKHTAKHHRKSHEDERSVEKETPRFTGDLGAVKDAIDLARRART
                     NDATDTEKTITDPAAQKLAEWFVLRHPESVAPFSRYAAFIAANPDWPGVLLMRRRAEA
                     RLWQEKADAATVHAFTNDRPVTAKGRFALARVLLGEGDRDGAQRLVREAFRSDELSER
                     AEEEAIDAFRDLLGREDYQARMDKRIGAKDLGAAMRAAHHLGDDAVSIVKACSAVKGG
                     DDKASDRLDSVAPDARADLGYVLCRVQWLMRKDRIADASRLVLSAPRQTMALQDTDEW
                     WRVRRILARKLLDGHDYQGAYEIVSTAALPASENYRADSRFMPGWIALRYLGDAKTAQ
                     MHFARLDEGCANPITLARASYWRGRAAEALGDKGAMRTHYEAAARHTTAYYGQLARAK
                     LGLAGMELRMPPPQPAGNSGVADEVVRAAQMLYTLGERETAVTFVTDLAEQSTDVPTL
                     SAVAEVTGRNNDARAMLELGKTALGRGLAVDAYAFPTIGIPQHNQYGQAIDLSVIYAV
                     ARTESAFNQRDRSSANAVGLMQVTPEAGRDTAKRFGVEYDWDRMVSDPVYNTQMGAAE
                     LSALLQEYKGSFMMTFAGYNAGRGRVRDWIQAYGDPRDPNVDPVDWAERIPFSETRNY
                     VQRVMENLMVYKHRFEEAAPAIAKSGPPRPAVARESANAAPTP"
     gene            6370..6561
                     /locus_tag="VM910_11230"
     CDS             6370..6561
                     /locus_tag="VM910_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63138.1"
                     /translation="MNYVTSITERTIWAVDYFLNRAMVYAWTSVLKRQQPLDEDDYGG
                     GDFCSLHETVSADDDQPLD"
     gene            complement(6606..>7076)
                     /locus_tag="VM910_11235"
     CDS             complement(6606..>7076)
                     /locus_tag="VM910_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HVI63139.1"
                     /translation="DGIGAALVRLNAFGAEAEHERNARLTSGPDTGPLLRTLLRLRHD
                     IVILGRALGHELPETVAGRLTAPLEQIESVASNFLRESGAALRARKPPPPLDTVEAAF
                     KTYIEEFGKVRRDGLTREMKSEAAERFFALGFALEQMREHLGEVHRVVGEWARD"
BASE COUNT         1228 a         2211 c         2263 g         1374 t
ORIGIN      
        1 acgatgtcga cgatcggccc cgaaatgccc ggacactcgt tgtagtcgcc gctcggaacg
       61 ccgccctcgt ggatcagcac gacgatggcc tcgatcccgc gtgcccgtaa atccggcacc
      121 agcgcgttca ccgtatcggc ctacggtttt acgaaacttt gcgcctttgg cttgcgccga
      181 atcttcctga acggacctcg tttccggcgc cgacaatttt cgcttgagct ttcttgaagc
      241 gttaaccgcc gtcggatgcc gtgctggcga tcggccgttt cacctctata aggaaggcca
      301 cacgcgagca tcgggaatgt cttacatgtc cagggaaggc aaatacgcgg catggtttcg
      361 cactccgcgc ggcgaaggca cgggtgtcgt gcatattgcg gaaggcaaga ttcgcggagg
      421 cgacagcacc ttcgactacg ccggctccta cgaaatcgat gaagatcaat ttaccgcaac
      481 gctgatgacc agaaggtacg ggtccggtcc gaccaccctg ttcgggtttg acgaggtcgt
      541 ggtgaagctc aagggatttt tcaaaggcag gacggtgttc tgttcggggg tggcggaaca
      601 ggcgccggac atccatttcg aagccacgct cttcccgagc catgacgagg tgagggcggt
      661 ggaggtgaag cgcgaggctg caaccttcga tgcggccaaa ttgccgaaat ccgaaggaaa
      721 tcgtcgtcgg gcgcggaacc cgtttgggac tggccgtccg atcgaccgga tgtgcacggc
      781 cggtccgccc cggcgaagct gacgatcacg ccgatccact ttacatcaat cgcgagtccg
      841 atggtctcgc gggccgctct ggcgtacgat agcgcctgcg gggcgcgcgc gcagcgccaa
      901 tgccccgatt gatgcagccg gaaggaggga tctcatgtac gccgccatcc gtcaggcgaa
      961 ggcaaagacc ggcaaggccg aggagctggc gacccggatc aaggagggcg cgattccgat
     1021 catcagcgat gtcgagggct ttatggccta ttacgtggtc tacgcgcccg acgacaccgt
     1081 gaccgcgatc tccgtcttca acaatttcgc cagcgccgag gaggcgaacc ggcgcgcgct
     1141 cgcctgggcc gaggagaacc tcgcgccgct gctcatcggg ccggcgacgg cggtggccgg
     1201 accggtgatc gtgcatacgc tggcctgacc gcaagggaac gggcggcggt tcgttgaccg
     1261 cggttcgggc caagttaggc gatgccataa aagaatgggc atggctcgtg ccatgcccat
     1321 tctgcctgct ggatcgtttt tgacctcgtc gtcggccgcc ttgcggcggg ccgatcgctt
     1381 cagtttgccg ctgacgccac cggcgtgccg gacgagggcg acttgagcga cggatccgga
     1441 tcgaagccga acacgccgac caggtatttg tagtattcgg acatcttttc cttcaggttc
     1501 tcgcgggtct tcggctcgtg gatcgactcc ttgccggcga ggaagatgct cgcggtgacc
     1561 gcaaagaatt cccggtcgtt cagcatcgta taggcgtttt tgccgaacag acccttggcc
     1621 ttggcttcgg catagaaggc cttgatgccg cggttgtcga atcccatcga catcagcctg
     1681 ccgtgatagg cgtgcaggaa ttcgtggagc aggatcggat cgtccgtgta ctgcatcatg
     1741 ttcggacgca gcatgatgac gcctcggccg gagtcggcgg cgagatcgac gacatccggg
     1801 ttgctccatt gctgcctgtc atggtcccag accgtgaggc cgagcgaggc atgacggcgg
     1861 cgcgtcggcg cgaccggacc gtagcaagcg atgcccgcgc cgagctcgag gcagtcggtt
     1921 tcgctggcga cgatcgggat ggtgtggaag aactccagca cccgcgggct gaagcctgcg
     1981 ttctccacca tgtcgagctg gtgtttgagg ttgtccgtga tcgcggcggc gtccttgcgt
     2041 ccggcatatt cggacagatc gtacatgtat ccgcgatatt tctcgaaatc cgcttccgtc
     2101 gtcggttgcg acgcctcctt cggcgcatcg accttgatga cgagcgaccc ggcgccggcc
     2161 ggcgtcgcac tcagcgtgat cgcgatcatc acgggaatca gtgatgcagc gcgcatgatt
     2221 attcctcctg ccgtctcttt gtttcgcgag cgcgctacca taagcgaccg gcattgcttt
     2281 gccgttagcc tggtaccaac gattgcattg ctgcaattat cccgaaatta accaagcggc
     2341 cggtgcgcgc gggccagcgg cgggggatca ggtttgcggg ccggagagcg aaatgtcctg
     2401 ctcggcgcgg aacacgttgg catagaggtt tgcgatccac tgccgtccgc tcgaggtcct
     2461 gttgagatcg ccgatggcat tctggatgtg cggcgacacg ctttcccaat agaattgcgg
     2521 gtaacgatcg acgatatcgg caggcgagcg atagccgagc tggcggttca tgccgacttc
     2581 ctcgaactcg tggtacagcg cgttggcttt cctgatgtag ttgggatcgc cgagctgccc
     2641 gatgaaatcg gccgctcgca cgatcgacgc ctctacgccg tagctctgcc cctcgttggg
     2701 aggaaatcgg gtgccctcga tggcgcttgc gacgcgctgc ttgtcgatca ggaaactgtc
     2761 cggaagtcgc tgcagaacgc agagtttcga gcggtcgacg tgatagggca tcaggctcgc
     2821 atccgacgag ccgcgcggca ggttcacctt gctgccgctc tggtccacta caaagccgcc
     2881 ctcattgtcg cccttcagcg cgccgcgaac atagccgatg tcgtgggtga ggcaggcaac
     2941 gatgacatgc gcgtaatcgg ccgcgatgac atgggtgtgc tgggcacggc cgcgcatgat
     3001 gtcgtgtccg gccagcgtca ccaataacgt gtgctcgata ttgtggtaaa gcgcatcgct
     3061 gttgccgatg cactccatcg caaggcgggc ggcgttcggc accgcttcca cgtacgccgc
     3121 ctctgccacc ccgaacctgc ggcgcataaa ggagctgaga aactcctcca aggcgtcggc
     3181 cgccaattcc ggtagagtca tcattggtgc tgccccgttc aggtattctt cccgcgtcca
     3241 attcctgact tctatgttac ctcccgttcg aaatcagcat agcgcatccg gagccgggcc
     3301 acacgcgcga atatcgtttc ggcgtgaacg gcgcgcctat attgcgccgc cgccaacgga
     3361 ggatcgtaag aggaggcgca agcttgttct gcaacgcctc ggccgcaagc ctgaacacgc
     3421 cagtggttcg ctaaagttcg aggtaatgcg ctaagccggg ctttacggga tcagcttaaa
     3481 tatgcaggcc gcgggattgt cgtttgaggc gacatcgccc gggcagcggc ggccccgagg
     3541 catcccatac cggcgcagtg caaccacgga aggtcagcat gactgtgcaa gagcgaaaga
     3601 aggtccagcg cgagccgcga cgccaactca atggccgcac cgcccagatg actgtcgacg
     3661 gtacgaccga gttcgaatgc tccgtgctcg acgttgggcc cggcggcgtc aagatcatga
     3721 cggaagccga gatcgacgtc ggcgccaggt tcgcgctggc actcgtaccg acgcatccgc
     3781 gacgtcagca atgcgaggtc gtttggcgcc gcggcaacac gttcggcgtc aaattcgtgg
     3841 cgtgacgctt cggctcccta tggagtaggt gccgcgtttg ccgattcccg cgcgacggcc
     3901 ggtcgcggcg gcccggattt cgcaatcgcc ggcgccgctt cttcgaagcg gtgcttgtac
     3961 accatcaggt tctccatcac gcgctgcaca tagttgcgcg tctcggaaaa cgggatgcgt
     4021 tcggcccagt cgaccgggtc gacattggga tcgcgcggat cgccgtaggc ctggatccag
     4081 tcacgcacgc ggccgcgacc ggcattgtag ccggcgaacg tcatcatgaa cgagcccttg
     4141 tattcctgca gcagcgcgct gagctcggcc gcccccattt gcgtgttgta gacgggatcg
     4201 gagaccatcc tgtcccagtc atactcgacg ccgaatcgtt tcgcggtgtc gcgaccggct
     4261 tccggcgtga cctgcatcag cccgactgcg ttcgcggagg agcggtcgcg ctggttgaac
     4321 gcgctttcgg tgcgtgcgac cgcatagatg acgctgaggt cgatcgcctg accgtactgg
     4381 ttgtgctgcg ggatgccgat ggttggaaag gcgtaggcgt cgaccgcaag cccgcggccc
     4441 aacgcggtct tgccgagttc cagcatggcg cgcgcatcgt tgttgcgccc ggtcacttcg
     4501 gccaccgccg aaagtgtcgg tacgtcggtg ctctgctcgg cgaggtcggt gacaaaggtg
     4561 accgcagtct cccgctcgcc gagcgtgtag agcatctggg cggcgcgaac gacttcatcc
     4621 gcgacgccgg aattcccggc cggctgcggc ggcggcatgc gcaactccat ccccgcgagg
     4681 ccaagcttgg cgcgcgccaa ttgtccgtaa tacgccgtcg tatggcgggc cgccgcctcg
     4741 taatgggtgc gcatcgcgcc cttgtcgccg agcgcctctg cggcgcgacc gcgccaatag
     4801 ctcgctcggg ccagcgtgat cggattggcg cagccctcgt cgaggcgggc gaaatgcatt
     4861 tgcgcggtct tcgcatcgcc gagatagcgc agcgcgatcc agcccggcat gaaacgggag
     4921 tcggcgcgat aattctcgct tgccggcagc gcggccgtgc tcacgatctc gtaggcgccc
     4981 tggtaatcgt gcccgtcgag cagcttgcgg gcgagaatgc ggcgcacccg ccaccattca
     5041 tccgtgtcct gcagcgccat ggtctgtctc ggcgccgaca gcaccaggcg gctcgcgtcg
     5101 gcgatcctgt ccttgcgcat cagccactgt acgcggcaca gcacgtagcc gaggtctgcc
     5161 cgcgcatcgg gtgcgaccga atcgagccga tcggaggcct tgtcgtcacc gcccttgacc
     5221 gcgctgcatg ctttcacgat cgaaaccgcg tcgtcgccga ggtgatgcgc ggcgcgcatc
     5281 gctgcgccaa ggtccttcgc gccgatgcgc ttgtccatgc gcgcctggta gtcctcgcgg
     5341 ccgagcagat cgcggaacgc gtcgatcgcc tcctcctccg cgcgctccga caattcgtcc
     5401 gatcgaaagg cctcgcgcac cagacgctgg gcaccgtcgc ggtcgccctc gccgagcagc
     5461 acgcgcgcaa gcgcgaagcg gccttttgcg gttaccgggc gatcgttggt aaacgcgtgc
     5521 acggtggccg catcggcctt ctcctgccat agccgcgcct cggcgcgccg gcgcatcagg
     5581 agaacgccgg gccagtcggg attggccgcg atgaaggccg cgtagcggct gaagggagcc
     5641 accgattccg gatggcgcag gacgaaccat tcggcaagct tctgcgcggc cggatcggtg
     5701 atggtctttt cggtgtcggt cgcgtcattg gtcctggcgc ggcgcgcgag gtcgatggcg
     5761 tccttcaccg ccccaaggtc gccggtaaag cgcggcgtct ccttctcgac ggatctttcg
     5821 tcctcgtgtg atttcctgtg gtgcttggcg gtgtgcttgc ctttcttctc ttcttttttc
     5881 agagaggcct tgcgttcttg cttcccgctg cttgcggcca gcgccttctt ctcgtgcgca
     5941 ggctccttgt ggcgcgcagc gagcgcaacg gtcgaagtcc cggcgagagc cgccaggacg
     6001 aacagacaag gcaacggccg gaaaagctgg ctcattccgt ggtcccctct gacccgccgg
     6061 tgaaccgcgg aatcacgcgg gttcgtccgg tgaaagcttc acacacggta tcatggcgtc
     6121 gatttgtaac tgtcgcgccc cggcaacagc ctgtgcaaat cgtttggcaa cagtgggaaa
     6181 aaattttgga acaattgagt ccaaaaaggt ctcacgtgca ctgtgtcgcc atgaagtgcg
     6241 gattcggggg tgggaaagat cgcagttcgg aagctcattg aagccgtgca gccaatgtga
     6301 gctgcggcac agtccgaaca caggactcgc gatttcaatc gctcgccaca ggatgcgatg
     6361 gggtgaagga tgaattacgt tacctcaatc acggagcgca cgatctgggc agtggattat
     6421 ttcctcaacc gcgccatggt ttatgcctgg acatccgtat tgaagcgcca gcaaccgctg
     6481 gacgaagacg attacggcgg tggcgatttc tgttcgttgc acgagaccgt ctcggcggac
     6541 gacgatcagc cgctggattg atggtttgaa aactcgtagc cgggcccgct ctgcgggccg
     6601 ctatcctaat cccgcgccca ttccccaacc acgcggtgca cctcgccgag atgctcccgc
     6661 atctgctcga gcgcgaagcc gagcgcaaaa aaccgttccg ccgcttcgct tttcatctcg
     6721 cgcgtcaggc cgtcgcgccg gactttgccg aactcttcga tataggtttt gaaagctgct
     6781 tcgaccgtat cgagcggcgg cgggggcttg cgcgcgcgca atgccgcgcc gctctcgcgc
     6841 aggaaattgc ttgccaccga ctcgatttgc tcgagcggcg cggtcagccg tcctgccacg
     6901 gtttccggca actcgtggcc gagtgcccga cccagaatca cgatgtcatg acgcaaccga
     6961 agcagggtgc gcagcagcgg tcctgtatcg ggaccggagg tgaggcgcgc gttgcgctcg
     7021 tgctcggcct cggcgccgaa cgcgttcaat cgcaccagcg ctgcgccgat cccgtc
//
LOCUS       DATLJT010000486         1937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1636754,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000486 DATLJT010000000
VERSION     DATLJT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636754"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VM910_11240"
     CDS             complement(<1..414)
                     /locus_tag="VM910_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI63140.1"
                     /translation="MTAVTKIRTDEGALRKARMLIGGKWVDSASGNVLEVEDPAHRRA
                     IAEVPRGGAEDVARAVQVAANAFPAWSRTIPRERGRLLARIADALEVRVEELARTIAL
                     ETGNALRTQARPEAKTTVDIIRYFGGLGSELKGETI"
     gene            complement(460..879)
                     /locus_tag="VM910_11245"
     CDS             complement(460..879)
                     /locus_tag="VM910_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63141.1"
                     /translation="MIRKKAAKKAPPRRAARTKTKPTAKVVVKPRPVSQRAQPETVEP
                     PPTRPSQPTAEEQRIGVVTHYYSHLSVLAMQLEPRTTLRVGDIIHIRGHTTDFTQKVE
                     SLEVNHAPVTEVAPSDDFGLKVVEHAREHDIVYKVRS"
     gene            complement(1412..>1937)
                     /locus_tag="VM910_11250"
     CDS             complement(1412..>1937)
                     /locus_tag="VM910_11250"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVI63142.1"
                     /translation="FAIRAVLLRPESRGFVRLKSADPCESPAIVQNFLSTQRDWEVLR
                     AGLRMAEEVGAQAPLRPFAGKRLAPLNVSDADLNKHVAETGITVHHPLGTCKMGVASD
                     PTAVVDTKLNVFGTEALRVVDASVMPDLVGGNINAPVIMIAERASDIVRGRVVLPRAT
                     ISEAAVKVTVMKAG"
BASE COUNT          336 a          612 c          595 g          394 t
ORIGIN      
        1 gatcgtttcg cccttgagtt cgctgcccaa gccgccgaag tagcggatga tgtcgactgt
       61 tgtctttgcc tcagggcgtg cctgggtgcg aagcgcgttc ccggtctcga gcgcaatcgt
      121 gcgcgccagc tcctcgaccc gcacctcaag cgcatcggca atccgcgcga gaaggcgccc
      181 gcgctcgcgg ggaatcgtgc gcgaccaggc gggaaaggcg ttggccgcga cttgcactgc
      241 gcgcgcgacg tcctcggcgc cacctcgggg gacctcggcg atcgcgcgtc ggtgcgctgg
      301 atcctcgacc tcgagcacgt tgcctgaagc gctatcaacc cacttcccac caatcaacat
      361 gcgcgctttc ctaagcgccc cctcgtcagt gcgaattttc gtaacagccg tcatgatcgc
      421 ctccgtcgtt ccttcggcag cttggtccgg ccaacggcgc tacgatcgca ctttgtacac
      481 gatgtcgtgc tcgcgcgcat gctcgaccac ctttagtccg aaatcgtcgc tcggcgcgac
      541 ctcggtcact ggcgcatgat taacctcgag ggactcgacc ttctgggtga aatcggtggt
      601 gtgtccgcgg atgtggatta tatcgccaac gcgcagtgta gtacgaggtt cgagctgcat
      661 ggccaagacc gagaggtggc tgtaatagtg ggtcacgacg ccgatgcgct gctcctcggc
      721 agtaggctgc gaagggcggg tcggcggcgg ctcgactgtt tcaggctgtg cccgttggct
      781 gactggcctc ggcttgacca cgacctttgc ggtcggcttg gttttggtgc gcgccgctcg
      841 ccggggcggc gccttcttcg ctgctttctt tctgatcatc ccagtttttc taaccggctt
      901 tctgcgcctt gccgtcttgg tcgctttctt gcgtttcgcc ttggctctag tttttgtcgc
      961 catggcagcc tcccttacgc tcactgcatt tggaaagtct tcgtccgatc gttgccgaat
     1021 aagaaggtaa gtcaacaggc cagaaggcca tcatcggccc tagaccgtcc acaggcgagt
     1081 gcagcaggtg actgcggtag cgtgaacaat cgctgctacg gaatcccgga acaggcacag
     1141 caatgtctga aatcccgctg cccggcagcc gagttgccgc aatgagttgg cagaggcagg
     1201 cttggacgaa cgcgctcgag gtaagcaact cgccccctga gcggcaaggc cgaggtttct
     1261 tgttagaaag gctcagagga gcaattgcag gccgacctgc tgccgatcgc ggatcggcga
     1321 ttcgtgccaa gagcggctat gagcagccgc agcaaaccat agcgcgggtt tcgtgagcta
     1381 catttgggcg gtcctcgatt cagccacacc tctaccctgc tttcataaca gtgaccttga
     1441 ctgcggcttc cgagatggtt gcacgcggca ggacaacgcg gccgcgaacg atgtcggacg
     1501 ctctctcggc tatcatgatg acgggcgcat tgatgttgcc gccgacaagg tccggcatca
     1561 ccgaggcatc gaccacgcgc aaggcttcgg tgccgaacac attcagcttg gtgtcgacca
     1621 ccgccgtcgg gtcgctcgcg acgcccatct tgcacgtccc gagcgggtga tgaacggtga
     1681 taccggtctc agcgacgtgc ttattcagat cggcatcgct gacgttcagc ggcgccagac
     1741 gctttccggc gaacggccgc agcggcgcct gggcgccgac ctcctcagcc atccgtaggc
     1801 cggcgcgcag aacctcccaa tcgcgctggg tggagaggaa gttctgcacg atcgccggtg
     1861 actcacacgg atcggcggat ttcaaccgca cgaaaccacg gctctccggc cgtaacaaca
     1921 cggcgcggat ggcgaag
//
LOCUS       DATLJT010000487         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_28855,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000487 DATLJT010000000
VERSION     DATLJT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28855"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..710
                     /locus_tag="VM910_11255"
     CDS             216..710
                     /locus_tag="VM910_11255"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63143.1"
                     /translation="MRRIGVLMSAASETDQQGSLAVFKETLHQLDWIDGRNMRVEVRW
                     ARGDPAEARKDAEELVALPADVIMTAGQLGLQALLQVTRSAPIVFNSIIDPVGSGYIE
                     SLARPGGNATGFITFDYALAAKWAELLKEIAPTVTRVAVLRDSATAAGIGQFTVGSTL
                     FRSL"
     gene            complement(1277..>2004)
                     /locus_tag="VM910_11260"
     CDS             complement(1277..>2004)
                     /locus_tag="VM910_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63144.1"
                     /translation="GYTIFLGGSTIHLTEMILREHPLLDPMKELTAVSIVAVNYYAIV
                     VNPTVPVHSLQELVSYVRANPGKLAYGSAGTGTVNHLTGELFKSLTGLTDLPHVAYRG
                     AGPAIADAIGAQIPIIVPAMTNQVLEFHRTAKLRLLAITSPNRIPVAPEISTAIEGGV
                     PDLVTQQVMALFAPAGTPKPIIAQIDEANHRAMADAAYRQSLVDAAVIPLPDWTAAQF
                     DEFMRTEIARWTPLVKAIGVRLD"
BASE COUNT          425 a          572 c          624 g          383 t
ORIGIN      
        1 ggggccgatc cggctacact gccgatcgaa cgtccgacga agttcgagac ggtgctcaac
       61 atgcgaaccg cgcaggctct gggaatatcc attcctacat cgcttttatt gcgcgccgac
      121 gaggtgatcg aatgaggcgg cgcgagttca taacactagt cggcgcgaca gcgatctctt
      181 ggccgaccag ggcgcgtgcg cagcaacgcg agcaaatgcg gcggatcggc gtgctaatga
      241 gcgcggcaag cgagacagat cagcagggca gtctcgcggt gttcaaggaa actctgcatc
      301 agttggactg gatcgacggc cgcaacatgc gggttgaagt ccgatgggcg cgcggtgatc
      361 ctgcagaggc tcgcaaagac gcggaagaat tggtcgcgct tccggcggac gtcattatga
      421 cagccggtca actggggctg caagcgctgc tgcaagtgac acgctcggcg ccgatcgtat
      481 tcaacagcat catcgatcca gttggcagcg gctacatcga gagcttggcc agaccgggtg
      541 gcaacgcaac tggctttatt acttttgact atgcgttggc cgcgaaatgg gcggagttgc
      601 tcaaggagat tgcgccgacc gtcacgcgag tggcggttct tcgggattcc gccaccgcag
      661 ccggaatcgg ccaatttact gtgggatcga cactcttcag aagtttatag aactgcgggc
      721 gactaacccc atgctggcgg gcgacccaac caatgatacg cgtccgcgtc ccgtgaggaa
      781 gcttcttgtc ggaaaacacg atggggagcg ctgcctgcca gaggcacagc acctccgcga
      841 tctcctgact cttcgtgata gttgcggcgg cgcgtgaccg ttgctgtacg cggtagcggt
      901 ggcggcgttg acgggcctgc tccatcaggc gctcaagtgc tgcgttgatg tcgcggagcg
      961 tcggtccgcc gagcaagagc gattcgtctc gcatgtcgca gtcatgccgc accgagcaac
     1021 cagcggcaac cagcaacagc ccggaagtgc ccgtaaaaca cagcagaaaa taaaacgaga
     1081 aacggccccg gagccaatat ggctggagcc gttttctggc gttattttaa ggtgttagga
     1141 gaaggttgcc tattttcccg agcagaaatc tcgtccgccc gtccccgcga aaagcgggga
     1201 cccgcattcg tagaagtcat tatcgcattt tctggatacc cgctcgcgcc ggaatgagcg
     1261 gaatcaaaac cgctatttag tcgagccgca cgccgatcgc cttcaccaac ggggtccagc
     1321 gcgcgatctc ggtgcgcata aactcgtcga attgcgccgc ggtccagtcc ggcagcggaa
     1381 tcaccgccgc atcgacgagc gattgtcgat aggcagcatc cgccatggcg cgatgattgg
     1441 cctcgtcgat ctgagcgatg attggctttg gcgtccccgc cggagcaaac agcgccatta
     1501 cctgctgggt caccaagtcc ggcactccac cttcaatggc cgtcgatatt tccggtgcca
     1561 ccgggatgcg gttcgggctg gtgatcgcca acaaacgaag cttggcggtc cgatggaatt
     1621 cgaggacctg gttggtcatc gccggaacga tgatcgggat ctgggcgccg atggcgtcgg
     1681 caatagcagg cccggcgccg cggtaagcga cgtgcggcag atcggtcaga ccggtcaagg
     1741 atttgaacaa ctcgccggta agatgattca cggttcccgt gccggcggag ccgtaagcaa
     1801 gcttgcccgg attggccctc acataggaca ccagctcctg caggctgtga acgggtaccg
     1861 ttggattgac aacgatggcg taatagttga ccgccactat cgacaccgct gtcaattcct
     1921 tcattggatc gagcaacgga tgctcgcgca gaatcatttc ggtgagatgg atcgtggagc
     1981 cgccaagaaa gatcgtgtag ccat
//
LOCUS       DATLJT010000488         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1407197,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000488 DATLJT010000000
VERSION     DATLJT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407197"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VM910_11265"
     CDS             complement(<1..321)
                     /locus_tag="VM910_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63145.1"
                     /translation="MMKRREFITLLGGAATAWPLAARAQQPARMRRIGVLVSTLPAGD
                     PEWQARSNAFVQGLQERGWSDGRNVRVEYRWGLGDPDRLRKYVAELVALAPDVILAAG
                     AAAVA"
     gene            complement(318..1313)
                     /locus_tag="VM910_11270"
     CDS             complement(318..1313)
                     /locus_tag="VM910_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63146.1"
                     /translation="MSLKRREFITLLGGTAAWPLAAWAQQADRMRLIGVLMAYAESDS
                     TAQSWLAAFRAALAKLGWTEGSNLRIELRWSAADADRMRTLAKELVDLRPNAIFGVTT
                     PAISALARETRTIPIVFTSVSDPIGSGFAASLAHPGGNITGFMANDPALGGKWVELLK
                     EIAPRTVRVALLFNPATAVPLQYFLPSIQAAASSFAVQANATPVHAKDEIEGVFAAQA
                     RNPGGGLIVMPDVFNEPNRELIIALAARYSVPAIYYNRFFSEPGGLISYGDARSEQFR
                     LAAGYIDRVLKGEKPADLPVQAPTRFELIVNLKTAKALGLTIPDKLLATADEVIE"
     gene            complement(1310..>2038)
                     /locus_tag="VM910_11275"
     CDS             complement(1310..>2038)
                     /locus_tag="VM910_11275"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63147.1"
                     /translation="EFVRLNVDVIVTYGTPAVAATKQATAVIPIVFAVAGDPVGSGLV
                     ASLPRPGGNLTGLSLQKTDVADKRLELLREVVPGLRGLAVMANAGNPVSILEMREVEA
                     TARRLGQEVATFEIRRVEDIAPTFEAFKGRTDALYVCGDPLVDTNRVRIITLALGARL
                     PSMSDFPEYVEAGGLMSYGPNFPDLFRRAAGYVDKILRGAKPGDIPVEQPTKFDFVIN
                     LITAKALGLDLSPTLLARTDKVIE"
BASE COUNT          406 a          639 c          615 g          378 t
ORIGIN      
        1 cgccaccgcg gcggcgcctg cggccaagat cacgtccggc gcgagcgcaa ccaattccgc
       61 cacgtatttg cgcagccggt cgggatcgcc caaaccccat cgatactcga cacgcacgtt
      121 tcggccgtcg gaccagcctc gttcctgcag gccttggacg aaggcgttgc tgcgggcctg
      181 ccattccgga tcgcctgcag gcaaagtgct cacgagcaca ccgatgcgcc gcattcgtgc
      241 aggctgctgc gcccgcgccg caagcggcca cgcggtagcc gcgccgccga gcagggtgat
      301 gaactcgcgg cgtttcatca ttcgatcacc tcgtcggcag tcgcaagtaa tttgtctggg
      361 atggtgagtc cgagcgcctt ggcggtcttg agattgacga tcaactcgaa ccttgtcggc
      421 gcttgcacgg ggagatcggc aggcttctcg cccttgagga cgcggtcgat ataccctgcc
      481 gctaggcgaa attgttcgct gcgagcgtca ccataagaga tcaggccgcc cggttctgaa
      541 aaaaagcgat tataatagat cgccggcacg ctgtagcggg ctgctagcgc aatgatcagt
      601 tcacgatttg gctcgttgaa cacgtccggc atcacgatga ggccgccacc cggattgcgc
      661 gcttgtgcgg cgaaaacgcc ttcaatctca tctttggcat gaaccggagt ggcattcgcc
      721 tggacagcaa aggaggatgc ggcagcttga atggacggta agaagtattg gagcggcacg
      781 gctgttgctg gattgaacag caacgcgacg cgcacggtgc gcggagcgat ctccttcaat
      841 agttccaccc attttccgcc cagtgcggga tcgttggcca tgaagccggt gatgttaccg
      901 cccggatgcg caaggctcgc agcgaagccg ctgccgatcg gatcggatac cgacgtgaac
      961 acgatcggaa ttgtcctcgt ctcgcgggca agggcgctga tcgcgggcgt ggtcacaccg
     1021 aagatcgcat tgggccgcag atcgaccagc tcttttgcca acgtcctcat cctatccgca
     1081 tcagcggcgc tccagcgaag ttcgatccgg agattgctgc cttccgtcca ccccaactta
     1141 gcgagcgcag cccggaacgc cgcgagccaa gactgcgcgg ttgaatcgct ctccgcatag
     1201 gccataagca cgccgatcag ccgcatccgg tctgcctgct gcgcccatgc cgcgagcggc
     1261 cacgcggccg tgccgccgag cagcgtgatg aactcgcggc gtttcaggct cattcgatca
     1321 ccttatcggt ccgcgcaagc agcgtcggcg agaggtcaag accgagcgcc ttggccgtaa
     1381 tcaggttgat gacgaagtcg aatttggtcg gctgttcgac ggggatatca cccggctttg
     1441 ccccgcgcag aatcttgtcg acatagccgg cagcacgacg gaataaatcc gggaaatttg
     1501 gtccgtagga catcagacct cccgcttcaa cgtattccgg gaaatcggac attgagggaa
     1561 gtcgcgcccc tagcgccaag gtaatgatac gaacccggtt cgtgtctaca agcggatcgc
     1621 cgcaaacgta aagtgcatcc gtgcggccct tgaaggcctc gaaggtgggc gcgatatcct
     1681 ccacgcgccg gatttcgaac gttgcgacct cctggcccag cctgcgcgct gttgcctcta
     1741 cctcacgcat ctcaagtata ctgacgggat tgccggcatt agccatgacc gccagaccgc
     1801 gaagacccgg aacaacttcg cgcaacagtt cgagccgctt gtcggcgaca tcggtttttt
     1861 ggagagaaag tccggtgagg ttaccgcccg gccgcggcag actcgcgact aggccgctgc
     1921 cgacggggtc tcccgccaca gcaaaaacga tcgggatcac cgccgtcgcc tgcttagtcg
     1981 ccgcaacggc gggagttcca taggtgacga taacgtcgac gttgagccga acgaactc
//
LOCUS       DATLJT010000489         3889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3475154,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000489 DATLJT010000000
VERSION     DATLJT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475154"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VM910_11280"
     CDS             <1..522
                     /locus_tag="VM910_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HVI63148.1"
                     /translation="RDLEEIKEFRATHGDIVMKPLFGHGGAAVFALTARDVNFGSLYD
                     MFAATFREPWVVQKYLPAVAKGDKRIILVDGEFAGAVNRVPQADDLRSNMVRGGVAQS
                     TDLTERERAICKQLGPALRERGLMFAGIDVIDGCLTEINVTSPTGVRAIKRLGGPDIA
                     VMIWDRIEAKRTS"
     gene            complement(616..2004)
                     /locus_tag="VM910_11285"
     CDS             complement(616..2004)
                     /locus_tag="VM910_11285"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI63149.1"
                     /translation="MAAQAAEIAALKARIAELERRLGLNSSNSGKPPSSDGLKKPSRV
                     KSTREPSDRPVGGQKGHKGGTLLQVAEPNKVVDHFPPACAACGSAMTLEMTVGHSARQ
                     VFDLPEPAPLIVTEHRAHNCRCACCGAQTRGSFPDAVSAPVQYGPRIAALVVYLLHYQ
                     LLPEDRLAELMTDLFGVRLVTATIARMSRSYATRLQDFVSVVRDLIAGAPVKHMDETG
                     LRIGGKTRWLHVAATALLTFYRVCQKRGSLLANVVGTVVHDHWKPYYTMTGVCHALCN
                     AHHLRELKALIDIEKEEWARAMHQLLRRACHAANLAAERGGALATLKPRLVAQLERRY
                     DAIVTAGLAFHQAQPALRHAAVTGARRLRGRTPRRTGHNLLLRFQLRKQDALRFLHDA
                     AVPFTNNQAERDGRMMKVKQKISGGFRSEQGAADFAIIRSFISTAKKHHWNIIQALLQ
                     DTQILIAVCRPT"
     gene            2322..3482
                     /locus_tag="VM910_11290"
     CDS             2322..3482
                     /locus_tag="VM910_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI63150.1"
                     /translation="MPQLNDLSRSLVTLEQDATLIAVIEMGQSSWPVAGIVPGVERQP
                     LKKLAVDEAALLKLLHRWRKEAEEAGHRITRITVGFEAGRDGFWLARWLRARDIEVHV
                     IHASSVAVSREHRRAKTDRLDTELLKRAFLGWLRGERDHCKMVAIPTINDEDAKRPNR
                     ERESLVGEQSRIINRIKATLARLGIRGFNPKLKKAAERLEGLRTPEGEPVPPNTLSEL
                     HRDMERRRLVRDQIRQIESARLDRLEHAPGEGVHAMVRLLARVMGIGVETADMLVQEV
                     LSRNLRDRRAVARYGGLTGSPDESGKKRREKGLARSGNPRVRRGMIQLAWRFLMFQKD
                     SALAQWFRARTENARGSRKTMIVALARKLLIALWRLVREGVVPDGVILRPAQ"
BASE COUNT          736 a         1150 c         1279 g          724 t
ORIGIN      
        1 cgcgacctcg aggagatcaa ggagttccgc gcgacccacg gcgacatcgt gatgaagcca
       61 ctgttcggcc acggcggcgc ggcggtgttc gcgctcaccg cgcgcgacgt caacttcggc
      121 tcgctctacg acatgttcgc agcgaccttc cgcgagccct gggtggtgca gaaatatctg
      181 cccgcggtcg ccaagggcga caagcgcatc atcctcgtcg acggcgagtt cgccggcgcc
      241 gtgaaccgcg tgccgcaggc tgacgatctg cgttccaaca tggtgcgcgg cggcgtcgcc
      301 cagtcaaccg acctgaccga gcgcgaacgg gcgatctgca agcagcttgg gccggcgctg
      361 cgggagcgcg ggctcatgtt cgccggcatc gacgtcatcg acggctgtct caccgaaatc
      421 aacgtgacct cgccgacggg cgtccgcgcc atcaaacgcc tgggcggccc tgacattgcc
      481 gtcatgatct gggaccgcat cgaggcgaag agaacatctt agcgttgcat accggttgaa
      541 ctcgcgcata gctaatcgga tggcgacctt tgattgttga aaaaatgtaa ctgcccagga
      601 agggtagtgg ggggttcaag ttggacggca gacagcgatc aggatttggg tgtcctggag
      661 gagcgcctgg atgatgttcc aatggtgctt cttggcggtt gagatgaaag aacggatgat
      721 ggcgaagtca gccgcgcctt gttcgctgcg gaagccgcca gagatttttt gcttgacctt
      781 catcatgcgg ccgtcgcgtt cggcctgatt gttggtgaac ggcacggcgg catcatgaag
      841 gaagcgcagc gcatcctgtt tgcgcaattg gaagcgcagg aggagattat gtccggtgcg
      901 gcgtggtgtg cggccacgta gtcggcgcgc gccggtgacg gcagcatggc ggagtgccgg
      961 ttgggcctgg tggaaggcaa gcccggcggt gacaatggcg tcgtaacggc gctccaattg
     1021 cgcgaccaga cggggtttga gtgtagcgag tgcaccgccg cgttcggcag cgaggttggc
     1081 agcgtgacag gcgcggcgta acagctggtg cattgcccgt gcccattcct ccttctcgat
     1141 atcgatcagt gccttgagtt cgcgcaggtg atgggcgttg cacagcgcat gacacacgcc
     1201 cgtcatggtg tagtagggtt tccagtggtc atgcaccacg gttccgacga cgttcgccag
     1261 caggctgccg cgtttttggc agacgcggta gaaagtgagg agggcggtcg cggcgacatg
     1321 cagccatcgg gttttgccgc cgatgcgcag gccggtctcg tccatgtgtt tgactggcgc
     1381 accggcgatg agatcgcgta ccacgctgac aaaatcctgc aggcgggtgg cgtagctgcg
     1441 actcattcgg gcgatcgtcg ccgtcaccag cctgaccccg aacaggtcgg tcatcaactc
     1501 ggccaggcgg tcttccggca gcaattgata atgcagcaga tagaccacca acgctgcaat
     1561 ccgcggacca tattgcaccg gcgcgctgac ggcgtcgggg aacgagcccc gcgtctgcgc
     1621 tccgcagcag gcgcaccggc agttatgggc acgatgttcg gtgacaatga gcggcgccgg
     1681 ctcgggcaga tcgaaaacct ggcgggcgct gtgcccgacg gtcatctcaa gcgtcattgc
     1741 tgatccgcag gcggcgcagg ccggcggaaa atgatcgacc accttgttgg gctcggccac
     1801 ctggagcagg gtgcctcctt tgtgaccctt ctgcccacca acgggccgat ccgatggttc
     1861 gcgcgtgctc ttgacccgcg acggcttttt gagcccgtcg ctcgacgggg gcttgccgct
     1921 gttcgaactg ttcaggccga gccggcgttc aagttctgcg atccgagcct tcagggctgc
     1981 aatctccgcc gcttgcgccg ccaccagcgc tatcagctct tctcgcgacg gctcactccg
     2041 cgaatcagca ttcggattca tcgaatcctt gaatcgaaag ctgattccgc tgtcgagaaa
     2101 aaaactacct gatcagtaac cttttgggaa actccgcgat tcgactcggg cggaaaacgt
     2161 tctagcagtc tgctgaaaaa cctctcgcgg cgcgacggga tttggtgcag cgaggctagc
     2221 tgccgttcca gcgggtgaaa gtcccgccaa cagagatgct atggggcgag cggcgaggat
     2281 aatggcacca tcgtcgctcc agcgaatcaa tggagcgccc catgccacaa ctgaacgatt
     2341 tgagtagatc cctcgtaact ctcgaacaag atgcgacact gatcgccgta atcgagatgg
     2401 gccagtcgag ctggcccgtt gcgggcattg tacctggcgt cgagcgtcag ccgttgaaga
     2461 agctcgccgt cgatgaggct gcattgctga agctgctgca tcgatggcgc aaggaagcag
     2521 aggaggcagg acacaggatc acacgcatca ctgtcggttt cgaggctggt cgggacggct
     2581 tctggctggc gcgctggctc agggcacgcg acatcgaggt ccatgtcatt catgcatcga
     2641 gcgtagccgt gtcgcgtgaa catcggcgcg cgaagaccga ccgtctcgac accgagctgc
     2701 taaaacgcgc ctttctcggc tggctacgcg gcgagcggga tcattgcaag atggtagcga
     2761 tcccaacgat caatgacgaa gatgccaagc ggcccaaccg cgagcgcgag agcctcgtcg
     2821 gagagcaaag tcggatcatc aatcgaatca aagcaacatt ggcccggctc ggcatccgtg
     2881 gcttcaatcc caagctaaag aaagctgccg aacgacttga aggcttgcgc acgcccgagg
     2941 gcgagccggt cccgcctaac acgttgtctg aactacatcg cgacatggag cgccggcggc
     3001 tcgtgcgcga tcagatccgg cagatcgaga gcgcacgcct ggatcgcctc gagcatgcac
     3061 ccggcgaggg agtgcatgcc atggtacgac tgctggcgcg tgtgatgggc attggtgtcg
     3121 agacggccga catgctggtg caggaggtgc tgtcgcgaaa cttgcgcgat cgacgcgccg
     3181 tagcgcgtta tggcggccta actggctcgc ccgacgagag cgggaagaag cgccgcgaga
     3241 aggggctggc gcggtccggg aatccccggg ttcggcgggg catgatccaa ttggcgtggc
     3301 gatttctcat gttccagaag gacagcgcct tggcgcaatg gttccgagcc cgcaccgaga
     3361 acgcccgcgg cagtcgcaag actatgatcg tggccttggc gcgtaagctt ctgatcgccc
     3421 tgtggcggct cgtgcgggag ggtgtcgtgc cggacggcgt cattttgcgc cccgcgcaat
     3481 gagctgacgc agaaagcacc taacagtttt ggcccacccc gtccgcttgt gacgggtcgc
     3541 cgatgacggt tcgaggtggc ggtggcccga tggactccat ggcttatcac gccgtaacag
     3601 agaatgggtc cccgccgctt cggagcttcg caaccgatgc gtattactgc atcatggttc
     3661 gggtccttgg acccaccgca tacaaggttg tggcgcgata gatccgcgct cgaacgcgag
     3721 ctcccctcta atcgtcttct tgctgaccta gccccgactg cattcgatgg tgccgccgtg
     3781 ctcggaactg tcaaggcagc cctgcgggtg gtcgccgaag aggcgaccag ccttgacagc
     3841 tcctgcgcac gacggcttgc cactgtggcg ggtcgggacg aagaaacgg
//
LOCUS       DATLJT010000490         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2096469,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000490 DATLJT010000000
VERSION     DATLJT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2096469"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VM910_11295"
     CDS             complement(<1..492)
                     /locus_tag="VM910_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI63151.1"
                     /translation="MEAMANSELARLSRDLESLNAKPLWERTTRMGPGSPAVPTIWRY
                     RDMRPQLLRAVELITAKEAERRVFMLENPGLPGTGYITNSLYCGLQVIKPGEIAPAHR
                     HTPNALRFIIEGEGAYSTIEGERVAMHPGDFVLTPGWTWHDHGNLGAAPVIWLDALDN
                     PFGQ"
     gene            complement(522..1499)
                     /locus_tag="VM910_11300"
     CDS             complement(522..1499)
                     /locus_tag="VM910_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63152.1"
                     /translation="MRRREFITLVLTLGLVLAIGLVAVQAHAQTWPQRTVRVILPLPP
                     GTATDIAARLFAQRLAERWGQSVIVENRQGGDGIPAVMSFLAARDNHTLMFSFAGVIT
                     INPLIHDKLPYDPASDLVPVASVVDNFFAIAASAALKVASLEDFIRLVRSQPGKLNWA
                     ATPGLPDYIFAALQRSAGIKMTQVSYRDFTPALQDLGEGRIHVAVSGLAFLLPQVQTG
                     KAKLLLVTNRERAPLAPDIPTAQEAGYPDLTFDGVVGLYGWRDMPADLKQRIAADVRA
                     VGADPTIGARITSAGSLVRLGGPAEFAAAIEEQRAKIAAIHATVAKPAQ"
     gene            complement(1786..>2005)
                     /locus_tag="VM910_11305"
     CDS             complement(1786..>2005)
                     /locus_tag="VM910_11305"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63153.1"
                     /translation="FQVQQDQVIALAARYRLPTIYEWSEFVRAGGLASYSADRVEMFR
                     QMGVYVSRMLNGAKPAELPVMQPTGLSS"
BASE COUNT          406 a          592 c          607 g          400 t
ORIGIN      
        1 ttgaccgaat ggattgtcga gcgcgtcgag ccagatcact ggcgctgcgc caagattgcc
       61 gtggtcatgc caggtccagc ccggcgtgag gacgaaatca ccggggtgca tggcaacgcg
      121 ctcgccctcg atggtcgagt atgccccttc gccttcgatg atgaaccgca gcgcgttcgg
      181 ggtgtggcgg tgtgctggag cgatctcgcc gggcttgatt acctgcagcc cgcaatagag
      241 cgaattggtg atgtagccgg tgccggggag gcccggattc tccagcatga acacgcgacg
      301 ttcggcttct ttggcagtga tgagctcaac cgcgcgcagg agctgcggac gcatatcccg
      361 gtagcgccag atggtgggaa cggcaggcga gcccggcccc atccgcgtgg tgcgctccca
      421 cagaggcttt gcgttcaagc tttcgagatc gcgcgacaag cgcgcaagct ctgaatttgc
      481 catggcttcc atgaaatcta tctcctcgcc gagatcactt ttcactgtgc cggtttagcg
      541 acggtggcat gaatggctgc aatttttgcg cgttgttcct cgatggctgc cgcgaattct
      601 gcggggccgc cgaggcgtac cagtgatccg gcgctcgtaa tccgcgcgcc gatcgtcggg
      661 tcggctccta cggcccgaac atcggctgca atgcgctgtt tgaggtcggc gggcatgtct
      721 cgccacccgt agaggccgac gaccccatca aatgtcaggt ccggatatcc cgcttcctgt
      781 gcggtcggaa tatccggggc caacggggca cgctcgcgat tggtcaccag aagcagcttg
      841 gccttgcccg tttgcacttg tggcagcaga aaagcgagac cgctcaccgc gacgtggatg
      901 cgtccttccc ccaaatcttg gagcgccggc gtgaagtctc gatacgaaac ttgggtcatt
      961 ttgatgccgg cgctcctttg caacgcggca aagatatagt caggaagccc cggtgtggcc
     1021 gcccagttca gcttgcccgg ttgtgagcgc accaacctta taaagtcttc cagcgaagcg
     1081 actttcaacg cggcggatgc cgcaatagca aagaaattat ctaccaccga cgcgacgggc
     1141 acgagatcgc tggcgggatc gtacggcaac ttgtcgtgga tcaatggatt gatcgtgatc
     1201 acaccagcga aagagaacat caatgtgtga ttgtcgcgcg ctgcaagaaa actcatgacg
     1261 gcagggatgc cgtcgccacc ctggcgattc tctacgatca cggattgacc ccaacgctca
     1321 gcaaggcgtt gcgcgaataa acgcgcggct atgtccgtcg cggtgccggg cgggagcggt
     1381 agaatcacac gaacggtccg ctgcggccag gtctgagcgt gcgcttgcac tgccacaagc
     1441 ccaattgcga gcaccagacc gagagtaagg accagcgtga tgaactcgcg tcgcctcatg
     1501 gtcgtcccct gtcgagggaa cccaccttac cgcatcgtcg aacgtaagct gtgttgtgca
     1561 gtacaacaaa ttcgagccgc taatgaccgc tttgggtcag aagcagcagg ccgtcaccta
     1621 gaccatgtcc gcttcgctct caaagcggac atcacgagct tgcccgatat gtccgttcag
     1681 tgccagaagc cgacattgtg ccgcctcaat cgatcacctc gtcggcgagt tgctggaatg
     1741 attcggttat tttcaacccg agagatttgg cggttttcag gttgattacg agctcaaacc
     1801 ggtcggctgc atcactggaa gctcggctgg tttggcaccg ttgagcatac gactgacgta
     1861 gactcccatc tgacgaaaca tctcgacgcg gtcggccgaa taggaggcaa ggccgccggc
     1921 cctgacgaac tcggaccact cgtaaatcgt cggcagacgg tagcgcgccg cgagcgcgat
     1981 gacctgatcc tgctgaacct gaaag
//
LOCUS       DATLJT010000491         5965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_258738,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000491 DATLJT010000000
VERSION     DATLJT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258738"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VM910_11310"
     CDS             <1..763
                     /locus_tag="VM910_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI63154.1"
                     /translation="AAEREAAAEVEGRKADLARANLNLDYATIRAPIGGIVGAAQVSE
                     GALVVQHDPMALATIQQLDPVYADFTQSVSELTRLRRAFESGDLERIAPDAIKVRLVL
                     EDGTLYPLPGRLLFSEAKVDANTGQVTLRGEFANPRRELLPGMYVRVRVDQGIDSDAI
                     AVPQQAIQRNSGGGSEVFVVGNDNRAAPQPVRTGAIQDGQWLVTEGLKAGDRVVVEGF
                     QKFAAGDKVTPHAWTQASAATEATATSRSSASIQR"
     gene            770..3928
                     /locus_tag="VM910_11315"
     CDS             770..3928
                     /locus_tag="VM910_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVI63155.1"
                     /translation="MPSFFIDRPIFAWVVALFICLVGAISIPLLSVAQYPIIAPPSIS
                     ISTSYPGASPENLYNSVTRLIEEELNGASGILNFESTSDSLGQVEITANFVPGTATSA
                     ASVEVQNRLKRIEARLPRAVIQQGILVEEASSAVLQIITLRSTDGSLDEVGLGDFMIR
                     NVLGEIRRIPGVGRATLYSTERSLRVWLDPAKLVGYGLTADDVTRAINAQNTQVASGS
                     VGAEPSPPEQRTATLVLVKGQLESPDEFGSIILRANPDGSTVRLRDVARIEIGGLSYQ
                     FNTRLNGKPTAGLSVLLSPTGNALATASAVEAKMKELSRFFPANIAYDIPYNITPVVK
                     ASIEKVLSTLVEAVVLVFVVMFLFLQNIRYTIIPTIVVPVALLGACATLMLAGYSINM
                     LTLFGMVLAVGILVDDAIVVVENVERIMAEEGLPPKEATRKAMSQITGAIIGITLVLM
                     AVFVPMAFFPGSVGIIYRQFSVTMVAAIGFSALLALSLTPALCATLLKPVEASHGHAR
                     KGLFGWFNRMLDNSRNRYVMTVQGALKRAGRLMLIYAALLIGLAYAFINLPGGFLPVD
                     DQGFITTDVQTPSDSSYARTQAAVEKVEKYLAERPGIDNVTFLTGFSFLGQGINTAQA
                     FITLKDWSERGPKDSAAAIVADINRDLSSVRDARVSALQPPPIDNLGNSSGFSFRLQD
                     RGQKGYPALMSAADRLIAEANASPVLQKVYIEGLPPAPQVNLVIDREKAGAFGVTFEE
                     INNTTSTNLGSNYINDFPNRGRMQRVIVQADSTSRMNADDILNYNVKNSKGQLVPFSA
                     FASVDWAKGPTQIAGFNYYPAIRISGEAKPGYTSGDAIAEMERLADRLPRGFGYEWAG
                     QSLQEKLSGSQAPFLLALSALVVFLCLAALYESWTIPLAVLLTVPLGILGAVLAAMMR
                     GLPNDVYFTVGLITIIGLAAKDAILIIEFAKDLRAKGMPLVEATIEACSLRFRPIVMT
                     GLAFVSGVLPMVIAKGAGGASQQALGTGVMGGMIAVVILALLMVPVFFVSVQRVLAGD
                     RPKAANKEVVDDRAARMHGAAE"
     gene            4089..5033
                     /locus_tag="VM910_11320"
     CDS             4089..5033
                     /locus_tag="VM910_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HVI63156.1"
                     /translation="MNAIWPDIPFKAWQETCSALHLYCQIVGKYRLARTPWVNHSWHA
                     TFYVNARGLTTSLIPDGTGVEIIFDLINHTVVGETAEGRRAEIPLGPMSVAEFHARFR
                     ELVEQLGGTPEFDGRPNEIADAIPFRDDRQRRPYDAAAVTRFFEALVSISLVFHRFRT
                     GFIGKVSPVHLFWGSFDLAVTRFSGRPAPLHPGGIPNLPDEVTREAYSDEVSSAGFWP
                     GGGGADFPAFYSYAYPTPKGFADAPVGPAGAYFDQKLGEFLLPYDVVRKAADPESTLT
                     AFLESTYRAAADLAGWDRDALECPIGQPLRPRVLKRSG"
     gene            complement(5056..5850)
                     /locus_tag="VM910_11325"
     CDS             complement(5056..5850)
                     /locus_tag="VM910_11325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVI63157.1"
                     /translation="MLHSALINVMVKAARRAGRSLKHDLGEIEHLQVSLKGPANFVSL
                     ADKRAEEMLYADLSKARPGYGFIGEEGGTREGADKSHTWIVDPLDGTTNFLHGIPQFA
                     ISIALAREGVVVAGVIYNPANDELYTAERGKGAFLNDQRLRVAGRRKLADCVIACGLP
                     HIGRGDHDQFRREMGEIQNKVAGLRRFGAASLDMAFVAAGRLDGYWERNLSPWDIAAG
                     QVVVREAGGIFTGADGHDNALTTGDVVCGNEFIHAELVTIMKAAAK"
BASE COUNT         1052 a         1923 c         1888 g         1102 t
ORIGIN      
        1 cgcggcggag cgcgaggcgg cggccgaggt cgaaggccgc aaggccgatc ttgcgcgcgc
       61 caacctcaat ctcgattacg ccaccatccg cgcaccgatc ggcgggatcg tgggtgcggc
      121 gcaggtgagc gaaggcgcgc tcgtcgtgca gcacgacccc atggcgctcg cgaccatcca
      181 gcaactcgac cccgtctatg ccgacttcac tcaatcggtc agcgaactca cgcggctgcg
      241 gcgcgcgttc gagtccggcg accttgagcg catcgccccc gacgccatca aggtgcgcct
      301 cgtgctggag gatgggacgc tttatcctct tcccggccgg ttgctgttct ccgaggccaa
      361 ggtcgatgcc aacacggggc aagtgacgtt gcgcggcgag ttcgccaatc ccaggcgcga
      421 actgctgccg ggcatgtatg tgcgcgttcg tgtcgatcag ggcatcgaca gcgacgcgat
      481 cgcagtcccg caacaggcga tccagcgcaa ttcgggcggc ggtagcgagg tctttgtcgt
      541 cggcaacgac aaccgcgcgg cgccgcagcc ggtccggacc ggagcaatcc aggatggtca
      601 atggctggtg accgagggcc tgaaagcggg cgaccgcgtc gtggtcgaag gcttccaaaa
      661 attcgccgct ggcgacaagg tcacgccgca cgcctggacg caagcgagcg ccgccaccga
      721 agcgacggca acgtcgcgat cgtcggcgtc gatccagcgg taagcgctca tgccgagttt
      781 cttcatcgac aggccgattt tcgcctgggt ggtcgcgctc ttcatttgtc ttgtcggcgc
      841 gatctcgatc ccgttgctgt cggtggcgca gtatccgatc atcgcgccgc cgtcgatctc
      901 gatcagcacg agctatcccg gcgcctcgcc cgagaacctc tacaacagcg tcacacgcct
      961 gatcgaggaa gaactcaacg gcgcatccgg catcctgaac ttcgaatcga ccagcgactc
     1021 gctggggcag gtcgaaatca ccgccaattt tgtgccgggc accgcgacca gcgcagcctc
     1081 tgtggaagtg cagaatcgcc tcaagcgcat cgaggcgagg ctgcctcgcg ccgttatcca
     1141 gcagggcatt ctggtcgaag aagcctccag cgcggtgctg cagatcatca cgctgcgttc
     1201 gacggatggc agcctcgacg aggtgggcct cggcgatttc atgatccgca acgtcctcgg
     1261 cgagatccgg cgcatcccgg gcgtcggccg cgccacgctg tattcgaccg agcgatccct
     1321 gcgagtctgg ctcgatccgg cgaagcttgt gggttacggc ctgacagccg acgacgtcac
     1381 acgggcgatc aacgcccaga acacgcaggt cgcttcgggc agcgtcggcg ccgagccgag
     1441 cccacccgaa cagcggaccg ccacgctcgt gctggtcaag gggcagctcg agtctccgga
     1501 cgaattcggg tcgatcatcc tgcgcgccaa tcctgacggt tcgacggtgc ggctgcgcga
     1561 tgtcgcccgc atcgagatcg gcgggctgag ctaccagttc aacacgcgcc tcaacggcaa
     1621 gccgaccgcg ggtctttcgg tgctgctgtc gccgacgggc aacgcgctcg cgaccgcgag
     1681 cgcagtcgag gccaagatga aggagctgtc gcgcttcttc ccggccaata tcgcctacga
     1741 cattccctac aacatcacgc cggtcgtcaa agcctcgatc gagaaggtgc tttcgacgct
     1801 ggtcgaagcc gtcgtgctcg tgtttgtcgt gatgttcctg ttcctgcaaa acattcgcta
     1861 cacgatcatt ccgaccatcg tggttcccgt cgccttgctg ggcgcctgcg ccacgctgat
     1921 gctggccggt tattccatca acatgctgac cttgttcggc atggtgctgg cggtcggcat
     1981 tctcgtcgat gatgcgatcg tcgtcgtcga gaacgtcgag cgcatcatgg cggaggaggg
     2041 gctgccgccg aaggaagcca cccgcaaggc gatgtcgcag atcaccggag ccatcatcgg
     2101 catcacgctc gtcctgatgg cggtgttcgt gccgatggcg ttcttcccgg gctcggtcgg
     2161 catcatctat cggcagttct cggtcaccat ggtcgccgcg atcggttttt cggcgctgct
     2221 cgcgctgtcg ctgacgccgg ctctctgcgc cacgctgctc aagccggtag aggcgagcca
     2281 cggccatgcc cgcaaggggc tgttcggctg gttcaaccgg atgctcgaca acagccgcaa
     2341 ccgctatgtg atgacggtgc agggcgcgct gaaacgagcc ggcagattga tgctgatcta
     2401 tgcggcgctg ctgatcgggc tcgcttatgc cttcatcaat ctgcccggcg gcttcctgcc
     2461 ggtcgacgac cagggattca tcaccaccga cgttcagacg ccgtcggatt cttcctacgc
     2521 gcgcacccag gctgcggtcg agaaggtcga aaaatatctc gcggaacggc cgggcatcga
     2581 caatgtcacg tttctcaccg gtttcagttt tctgggccag ggcatcaata cggcgcaggc
     2641 cttcatcacg ctgaaggact ggtcggagcg ggggcctaag gattccgcgg ctgcgatcgt
     2701 cgccgatatc aaccgcgatc tgtcgtcggt gcgcgacgcc cgagtctctg cgttgcagcc
     2761 gccgccgatc gacaatctcg gcaactcctc gggtttcagc ttccgcctgc aggatcgcgg
     2821 gcaaaagggc taccccgcgc tgatgagcgc ggccgaccgg ctgatcgccg aagccaatgc
     2881 gagtcccgtc ctgcaaaagg tctatatcga gggcctgccg cccgcgccgc aggtcaatct
     2941 cgtcatcgat cgcgaaaaag ccggcgcgtt tggcgtcacc ttcgaggaaa tcaacaacac
     3001 gacctcgacc aatctcggct cgaactacat caacgacttc cccaatcgcg gccgcatgca
     3061 gcgcgtcatc gtgcaagccg acagcacgag ccgcatgaat gccgacgata tcctcaacta
     3121 caacgtcaag aacagcaaag gccaactcgt gccgttctcg gcgttcgcgt cggtcgactg
     3181 ggccaagggg ccgacccaga tcgcgggctt caactattat cccgcgatac gcatcagcgg
     3241 cgaggcaaag ccgggctaca cgtccggtga cgcgatcgcc gagatggaac ggctcgccga
     3301 caggctgccg cgcggattcg gctacgaatg ggccgggcag tcgctgcagg aaaagctctc
     3361 gggctcgcag gccccgttcc tgctcgcctt gtcggcgctg gtcgtgttcc tctgcctcgc
     3421 cgcgctctat gagagctgga ccattccgct tgcggtcctg ctcacggtgc cgctcggcat
     3481 tctcggcgcg gtgctggcag cgatgatgcg cggcttgccg aacgacgtgt acttcaccgt
     3541 ggggctcatc accatcatcg gattggctgc caaggacgcg atcctgatca tcgagttcgc
     3601 aaaggacttg cgcgccaaag gcatgccgct ggtggaagct accatcgagg cgtgcagcct
     3661 gcgcttccgc cccattgtga tgacgggcct cgcctttgtc agcggcgtat tgcccatggt
     3721 gatcgcgaag ggtgcgggcg gcgccagtca gcaggcgctc ggcaccggcg tcatgggcgg
     3781 catgatcgcc gtcgtgatcc tcgcgctgtt gatggtgccc gtgttcttcg tgagcgtgca
     3841 acgggtgctg gcgggcgacc gcccgaaggc agcgaacaaa gaggtggtcg acgatcgagc
     3901 ggctcgcatg catggcgctg cggaatagcg actgaggccg caaccgtggc cactgcgcgc
     3961 caacaagtgt ttacgccaat cggcagacca ttgtatcctc gcgcaccgga accttcagtg
     4021 agcgtggaag cgatggcgcg gcctctctcc ggctttcagg aatggccgca cacgaggagg
     4081 gagccgccgt gaatgcgatc tggccagaca ttccgttcaa ggcatggcaa gaaacctgtt
     4141 ccgcgcttca tttatattgc cagattgtcg gcaaataccg actcgcaagg acgccgtggg
     4201 tcaaccattc gtggcacgcg accttctacg tcaacgcacg tggcctgacg acgtccttga
     4261 ttccggacgg gacaggcgtt gagatcatct tcgatctcat caatcacaca gtcgtcgggg
     4321 agacggcgga aggacgccgg gccgaaatcc cgctcgggcc gatgtccgtc gccgaatttc
     4381 acgcccggtt ccgcgaactg gtcgaacagc ttggcggcac gccggagttc gacggtcgac
     4441 ctaacgaaat tgccgatgcg atcccgtttc gggacgaccg gcaaagaaga ccctatgatg
     4501 ccgccgccgt gacgcgcttc ttcgaagctc tggtttcgat cagccttgtc ttccatcggt
     4561 ttcggacggg gttcatcgga aaagtcagtc ccgtccatct tttttggggc agcttcgatc
     4621 ttgcggtgac ccgcttctcc gggcgtcctg ctccgcttca cccgggcggc attccaaatt
     4681 tgcccgatga ggtcacgcgc gaggcctata gcgatgaagt ttcctcggcc ggcttctggc
     4741 cgggcggcgg cggggccgat tttccggcgt tctactccta cgcctacccc accccaaagg
     4801 gatttgcgga cgcgccggtt ggacccgccg gcgcctattt cgatcagaag ctcggcgaat
     4861 tccttttgcc ttatgacgtc gtgcgcaaag cggccgaccc ggaatcgacg ctcacggcgt
     4921 ttctcgagag cacttatcgg gcggcggccg atctcgcagg ctgggaccgt gacgcgctgg
     4981 aatgtccgat cggccaaccg ctgcgaccgc gagtgctgaa gcgaagcggt tagcgcgacg
     5041 gcgcgtgggt gcgatttact tcgcagccgc cttcataatt gtcacgagct cggcgtgaat
     5101 gaattcgttg ccgcagacga catcgccagt cgtcagcgca ttgtcatggc catcggcgcc
     5161 cgtaaagatg ccgccggcct cgcgcaccac gacctggccg gccgcgatgt cccacggcga
     5221 gaggttgcgt tcccaatagc cgtcgagccg ccccgcggcg acgaaggcca tgtcgagcga
     5281 ggccgcgccg aaacgccgga ggcctgcgac cttgttctgg atctcgccca tctcgcggcg
     5341 aaactgatcg tggtcgccgc gcccgatatg cggcaaccca caggcaatta cgcaatcggc
     5401 gagcttgcgg cggccggcga cgcgcagccg ctgatcgttg agaaacgcgc ccttgccgcg
     5461 ttcggcggta tagagctcgt cattggcagg gttatagatc acgcccgcga ccacgacgcc
     5521 ctcgcgcgcc agcgcgatcg agatggcaaa ttgcgggatg ccgtgcagaa aattggtggt
     5581 gccgtcgagc ggatcgacga tccaggtgtg ggatttgtcg gcgccctcgc gcgtgccgcc
     5641 ttcctcgccg atgaaaccgt agcccggccg cgccttggaa aggtcggcgt agagcatctc
     5701 ttcggcgcgc ttgtcggcaa gcgagacgaa attggccggc cccttcagcg aaacctgcaa
     5761 atgctcgatc tccccgagat cgtgcttcag gctgcggccg gcgcggcgcg cggccttgac
     5821 catgacgttg atgagggcgg agtgcagcat cagctcggca aatctgtttc ggcagaaaga
     5881 atgcgccggg gaggtgagag gtcgggaagt gggtgccttg cgatcgcgcc gccgtcaagg
     5941 tgcatcattt cacgttgagc cagcg
//
LOCUS       DATLJT010000492         4756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_29193,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000492 DATLJT010000000
VERSION     DATLJT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29193"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1385)
                     /locus_tag="VM910_11330"
     CDS             complement(126..1385)
                     /locus_tag="VM910_11330"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVI63158.1"
                     /translation="MRRREFIALIGGAAASVGVRNVLFARDQTLPSDALEGRLADIIA
                     AYDSQGNHRTATSVDDASAAWLAKQVRQAGAEPSLEPFTLSRIDPLSCYVRIADRRID
                     GVPLFDASFTGAHGVRGTLGPIGSDAEIGLAETEPSRLTQPGSEVRRARLAEVRQARH
                     RAVILITRGIRPGLFLLNAPAFKAPVGPPVLQVSSAEAEWLRAQAQQHAEVALVAHVE
                     RTLAQAFNVVTQIAGSDPGLAPLVITTPRSGWWQCASERGGGIACWLETIRLLSAEKP
                     ARDCLFVAMSGHELGGLGGQAYLENRGNLAKRALVFIQFGANIGAPRQPNLIQASGAA
                     LEQWAVAAMEREGLAVDRKAAQDATPFGEAAIFKRAGGRYVAPVCDSDVFHNQADRWP
                     DAVDVAALARYAKAFAYGALQLAQQRG"
     gene            1596..2309
                     /locus_tag="VM910_11335"
     CDS             1596..2309
                     /locus_tag="VM910_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI63159.1"
                     /translation="MTIGVVFAAAFTISGAAQDFIAQSGAPAAAFPKPDRPVADIVSP
                     IWHDEKERDDAGEPSQLVRLLGIQSGMTVVDIGAGSGYYVVRLSPVVGSKGRIIAEDI
                     VPKYLQSLRARVRKLGLQNVVISLSEAHDPKLPADSVDIAILVHMYHEIEQPYALLYN
                     LVPALRLQARVGIVDAFAPTSKHGTPPGLLRCELAAVGYREISFDRLIGSDAYLAIFA
                     PPSVASRTRPEAIVACKVQ"
     gene            complement(2350..2760)
                     /locus_tag="VM910_11340"
     CDS             complement(2350..2760)
                     /locus_tag="VM910_11340"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HVI63160.1"
                     /translation="MSDASRLLFLRIALIVIGLTFIFGIYTLGVLWPSGWTWGQGHSH
                     YLMMIIGVYATLGVFLLIAARNPYAHKSLIWFTVWSSVVHAAIMGVQALGDPAERGHL
                     VGDVPALILVAIVLAALTPRGLHQLESDRRTVAA"
     gene            complement(2942..3226)
                     /locus_tag="VM910_11345"
     CDS             complement(2942..3226)
                     /locus_tag="VM910_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63161.1"
                     /translation="MIEVQRTAENDSLVFDVVVREGKGETRHHVIMSREMCERLTAGK
                     HTPERCVEAAFRFLLDREPKESILARFDVTAISRYFPEFEREMPRYFSQF"
     gene            3415..3882
                     /locus_tag="VM910_11350"
     CDS             3415..3882
                     /locus_tag="VM910_11350"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI63162.1"
                     /translation="MAVLLRPPAMFERALNAFWILLGAAAAAYAWTLGLIGASGPESG
                     LFPFLAGLIIMGAGVVLLLRPLSEHIAPDFPRGDALWRVLGVIAGLAFMAISIPLLGF
                     AVAGFITMVILLRTVEKSSWWFSLALALVAIVVVVWLFGRILGMALPRGPWGW"
     gene            3886..>4756
                     /locus_tag="VM910_11355"
     CDS             3886..>4756
                     /locus_tag="VM910_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI63163.1"
                     /translation="MEAIGGLLTGFGIILTLDNLFVCFLGSMIGTLVGVLPGVGPLAA
                     LALLLPITFTMTPVGGMVMLASIFYGAMYGGSTTSILLNIPGEAASVVTCLDGHQMAK
                     QGRAGAALGIAAIGSFVAGTLGVIVMTFFAPTIATVAIRFGPPENFALMVLGIVCTLF
                     MITGSPVKGALMVALGFLAAAVGIDVVNGRERFTFGSVDLSAGIELLAVVIGLFGVTE
                     VLNNVERVVKSTLVAERVRGLWPTLADWRASWRPMLRGSGLGFLLGLVPGGGPVTASF
                     MSYALEKRLSAHPD"
BASE COUNT          870 a         1506 c         1429 g          951 t
ORIGIN      
        1 tgcggctggc acgccagcag cgatgaggtg cgccaccaca ccaatgagac cgagggtcct
       61 acgcttcacg gcttcctcct gtgagatctt aagcaatggc ccggcaacat tgactgtgaa
      121 gactctcaac cccgctgctg cgcgagttgc agcgcgccgt aggcaaaggc cttggcgtag
      181 cgggcgagtg ccgccacgtc cacggcatcc ggccaacgat ccgcttggtt gtggaagaca
      241 tcgctatcgc acaccggagc aacatatcgc ccgccagcgc gcttaaagat cgcagcctcg
      301 ccaaaaggcg tagcatcctg cgcggccttc ctgtcgacgg cgagcccctc tctttccatg
      361 gccgcgacag cccattgttc aagcgcggcg ccggaggcct gaatgagatt gggctggcgc
      421 ggtgcaccga tgttcgcgcc aaactggatg aagacgagcg cacgtttggc caagttccca
      481 cgattctcga gataggcttg tccgccgagt ccgccaagtt catgcccact catcgccacg
      541 aaaagacaat cccgcgccgg cttttccgca ctcagaaggc ggatcgtttc aagccagcaa
      601 gcaattccgc cgccgcgctc gctggcgcac tgccaccaac cacttcgcgg ggtcgttatg
      661 accagcggcg ccagaccggg atcgctgccc gcaatttggg tcacaacatt gaatgcttgc
      721 gccagagtcc gctcgacgtg ggcaacgagg gcaacttccg catgttgttg cgcctgcgcc
      781 ctcagccatt cagcttctgc gctcgaaacc tgtagcaccg gtggaccaac cggcgcctta
      841 aaggccggcg cgttgagtag gaacaacccc ggcctaatcc cgcgcgtgat gaggatcact
      901 gctctatgtc gcgcctgccg cacctccgcg agtcgggcgc gccggacttc gctccccggc
      961 tgtgtgaggc gggacggttc ggtttccgcc aatccaatct cggcatcgct accaatcgga
     1021 ccgagcgtgc cacgcacacc gtgtgcgcca gtaaagcttg cgtcgaacaa cggtaccccg
     1081 tcgatgcgcc gatctgcgat gcgcacgtaa cacgataatg gatcgatgcg actgagtgtg
     1141 aaaggctcga gcgacggctc ggcacccgcc tgccgtactt gtttggccag ccatgcggcc
     1201 gatgcatcgt ccaccgacgt tgccgtgcgg tggttccctt gcgagtcgta agccgcaatg
     1261 atgtccgcga gccgtccttc cagcgcatcc gacggcagcg tctgatccct tgcaaacagg
     1321 acgttacgaa ctccgaccga ggccgccgca cctccgataa gcgcaatgaa ctcgcggcgt
     1381 ctcataggct tacccaaccc cgagggcgac ggagcaacta tagcaggtca gggccgtgca
     1441 tcgcagcaga aagcggccac tcatgtctgc tttgggccaa acgctcctgt ccaagctgag
     1501 cctccgtgtg ccctgaggtc cgcagctctc caaaagcgga cattgaccgg ataataaggg
     1561 gtctcagatc gaccgtgggc cacctcatga tgcgaatgac aattggtgtc gtctttgccg
     1621 cagcattcac gatttcaggc gctgcgcagg atttcatcgc gcaatcgggc gcgccggcgg
     1681 ccgcctttcc gaaaccggat cgaccggtcg cggacatcgt cagtccgatc tggcacgacg
     1741 aaaaggaacg cgacgatgcc ggcgaaccga gccaactcgt gcgcctactc ggcatccaat
     1801 ccgggatgac tgtcgtggac atcggcgccg gtagcggcta ctacgtcgta cggctgtcgc
     1861 cggttgtcgg ttccaagggc cgcatcatcg cagaagatat cgtccccaag tatttgcaaa
     1921 gcttgcgcgc gcgagtgcgc aagctcggat tgcagaacgt tgtgatcagc cttagtgagg
     1981 ctcacgatcc gaagctgcca gccgactcgg tggacattgc aattctcgtg cacatgtatc
     2041 acgagatcga acaaccttac gccctgctct acaatctcgt ccccgcgctc aggctgcaag
     2101 cgcgcgtcgg cattgtcgac gcctttgcac cgacatcaaa gcacggcacc ccaccaggcc
     2161 tgctgcgctg cgagctcgcg gccgttggct atcgcgagat cagtttcgat cggctcatcg
     2221 gcagcgacgc ctatctcgcg atttttgcgc caccgtccgt tgcaagccgc acgcgaccgg
     2281 aagcgatcgt cgcgtgcaag gtgcagtagc ggatcgctta tgacagaagt tgggccaaat
     2341 tcgacaaaat caggccgcga cggtgcgacg gtcactctca agctggtgca gtccgcgcgg
     2401 cgtcaacgct gcaagtacga tcgccacgag aataagcgct ggcacatctc ccacgagatg
     2461 accgcgttcg gccgggtcgc cgagggcctg aacgcccatg atcgccgcgt gaaccacgct
     2521 cgaccacacg gtgaaccaaa taaggctctt gtgagcgtag ggatttcttg cggcgatgag
     2581 caaaaacacg cccagcgtcg cgtacactcc gatgatcatc atgagatagt gcgaatgtcc
     2641 ctgtccccaa gtccagcccg agggccagag aacacccaat gtatatatgc caaatatgaa
     2701 agtcagaccg atgacgatca gggctatgcg cagaaaaagc aggcgacttg catcagacat
     2761 tttgcacctc cctgaactgg cggcccaatt cttgaaccgg aacgtgttct ggatgtcacg
     2821 agctccattt ttcttcccta tggttgcagg agcacaacgg agccgaggtt agcactccat
     2881 ccccgaaagc gcacatcctc ggcaagcgac ccagaaaaaa taacccggca tgcatatttt
     2941 atcagaactg tgaaaagtaa cgcggcatct cccgctcgaa ctcggggaag tagcgcgaga
     3001 tcgcggtcac gtcgaagcgc gcgaggattg attccttggg ctcgcggtcg agcaagaacc
     3061 ggaacgccgc ctcgacacag cgttctggcg tatgcttgcc cgccgtcagc cgctcgcaca
     3121 tttctcgcga catgatgacg tggtgacgag tctcgccctt tccttcgcgg acgaccacgt
     3181 cgaaaacgag agagtcgttc tcggcagtgc gctggacctc aatcatgtcg atctccttcg
     3241 cgatgctcgc gcattaaacg gctccgagcc gggttgccaa tgggacgcgt caccgcccga
     3301 tcttggagca gcagaatgac attgtgcgcc ggagtcctta cactcgcctg actccaaggc
     3361 caccgaggcc gccaccatcg ccccccgccg catagcgcca cgcgcgaggc cgcgatggcc
     3421 gtcttgctta ggccgcctgc catgttcgag cgcgcgctca acgcattctg gattttgctc
     3481 ggggctgccg cagcagccta tgcgtggacc ctcggcttga tcggcgcgtc cggtcccgaa
     3541 agcggcttgt tcccctttct tgccggcttg atcatcatgg gtgcgggcgt cgtgctgctg
     3601 ttgcggccgt tgagcgagca tattgctcca gacttcccgc gtggcgatgc gctgtggcgc
     3661 gtccttggag tcatcgccgg gcttgctttc atggcgatca gcattccgct tctggggttt
     3721 gcggttgccg gtttcataac gatggtcatc ctgctgcgca ccgtggaaaa atccagttgg
     3781 tggttctcgc tcgcgcttgc cttggttgcc atcgtggttg tggtttggtt gttcggccgg
     3841 atactgggca tggcactgcc gcgaggtccg tggggttggt gaggcatgga ggccatcggg
     3901 gggctgctca ccggtttcgg tatcatcctc acactcgata atctttttgt ctgctttctc
     3961 ggcagcatga tcgggacgct ggtgggcgtg ctgccgggcg tgggcccgct cgcggcgctc
     4021 gccttgctgt tgccgatcac gttcaccatg acgcctgtcg gcggaatggt gatgctcgcc
     4081 tcgatctttt acggcgcgat gtatggcggc tcgacgacct caatccttct gaacatccca
     4141 ggcgaggccg cgtcggtcgt gacctgtctc gacggtcacc agatggcgaa gcaggggcgc
     4201 gccggcgcgg cactggggat cgcggcgatc ggctccttcg tcgccggcac actcggcgtg
     4261 atcgtgatga cgttctttgc gccgacgatc gcgacggtcg ccattcgttt cgggccgcct
     4321 gagaatttcg ccctgatggt gctcggcatc gtttgcacgc ttttcatgat caccggctcg
     4381 ccggtcaagg gcgctctgat ggtggcgctc ggatttctcg ctgccgcggt tgggatcgac
     4441 gtcgtcaacg gccgcgagcg cttcaccttc gggtcggtcg atctttccgc gggcatcgag
     4501 cttctggccg tcgtcatcgg gctgttcgga gtgacggagg tgttgaacaa cgtcgagcgg
     4561 gtcgtcaaaa gcaccctcgt cgccgagcgc gtccgcggct tgtggccaac gttggcggac
     4621 tggagggcct cgtggcggcc catgcttcgc ggcagtggat tgggattcct cttgggtctg
     4681 gtccccgggg gcgggccggt gaccgcgtcg ttcatgtcct acgccctgga gaaacggtta
     4741 tccgcacacc ccgacg
//
LOCUS       DATLJT010000493         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3934677,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000493 DATLJT010000000
VERSION     DATLJT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934677"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VM910_11360"
     CDS             <1..448
                     /locus_tag="VM910_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63164.1"
                     /translation="EVDGNRMRRIYMDGRQHPEDPDLTLHGHSIGHWEGDTLVIDTTA
                     IVPQAYIAISEAVGIPNNGDMHIVERLHLTAPNVLSDEMEITAPKVLSTTWKTTRNFR
                     RYPDRHYEITEGECEQEDLIPGKDQFGNDIFVPNAQQAYGTVHTVK"
     gene            461..853
                     /locus_tag="VM910_11365"
     CDS             461..853
                     /locus_tag="VM910_11365"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HVI63165.1"
                     /translation="MPRKVVVLAAALMLCFAGAAPAHHSNAAYDLEHQQTVEATVKQV
                     NWTNPHISFVVETDAKDGKPTETWVFEVSSPGVLTRSGWNKRSLQVGDHALFQYAPLR
                     DGRPGGFLRKVTLPSGQELTYSLQPEEQ"
     gene            861..1364
                     /locus_tag="VM910_11370"
     CDS             861..1364
                     /locus_tag="VM910_11370"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HVI63166.1"
                     /translation="MTAVLSALSKWLAATSLSHTIQSVGWIIPTLQTIHILCVAVVFS
                     SAVLVDFRIYRLFERDVPLAEISRRFLRAIWPVLLVLLVTGSLLIIGEPRRSLLNSTF
                     YIKMSLLLAAVLLTAALQGSIAASPGFWEKDRGRRMAGKALATLSIVIWCGILFAGRW
                     IAYTQAG"
     gene            1364..1867
                     /locus_tag="VM910_11375"
     CDS             1364..1867
                     /locus_tag="VM910_11375"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HVI63167.1"
                     /translation="MSLFLDVIGYFENSTVADAIRENDVLFPTIESVHVVAICLVVGS
                     ILVLDLRLLGFASLQRPVSRLAGAILPITWGAFTLAAVSGFLLFMSNATKYLANGYFV
                     AKMFLICAAGANMIIFHLISARDLPQWEKDASPPLLVRTAGALSILLWISVVACGRWI
                     GFTMRPL"
     gene            1869..2333
                     /locus_tag="VM910_11380"
     CDS             1869..2333
                     /locus_tag="VM910_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63168.1"
                     /translation="MGSLSRPSGFAAAIVVASLAASPSLSEPPPTAPFRAHVDMKAFM
                     EHVLQPAASLIWTVNGVMIDEKGEHDLGPKSDDDWEKLVSAAATLAEATNALMIPQRA
                     LDPDWNRYVERLADAADKAYRAAEAHDLKSISEVSDRLDGICAACHRHYGVE"
     gene            complement(2403..>3447)
                     /locus_tag="VM910_11385"
     CDS             complement(2403..>3447)
                     /locus_tag="VM910_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63169.1"
                     /translation="TTGPAAALGIPERNALEFVPKEIAGIPLKVIVLDDGGDPTNATT
                     NARRFVTESKADIIMGSSTTPPTIAVSNVANEAGIPHIGLAPFPITEARGKWSVDMPQ
                     PVPIMGKVLYEHMKAHNIKTVGYIGYSDSYGDLWFNDFKNQGVPMGMTVVDEERFARP
                     DTSVTGQVLKLIAANPDAILVGASGTAAALPQTELRDRGYKGLIYQTHGAASMDFIRI
                     AGKAAEGVIMASGPVMSPETQPDTALTKKPGLALDTAYEAKYGPNSRSQFAGHSYDAF
                     EVLKRVIPTALKTAKPGTPEFREAIRQAFLTEREIPASQGVYNFTEKDRYGLDDRSRI
                     ILTVKDGKYIPAN"
BASE COUNT          611 a         1080 c         1066 g          690 t
ORIGIN      
        1 cgaggtcgac ggcaaccgca tgcggcgcat ctacatggat ggccgtcagc atcccgaaga
       61 tcccgatttg acgctgcacg gccattcgat cggccattgg gaaggcgata cgctcgtgat
      121 cgacaccacc gcgatcgtgc cccaggccta tatcgcgatc agcgaggcgg tcggcatccc
      181 caacaatggc gacatgcata tcgtcgagcg gctgcatttg acggcgccga acgtgctctc
      241 cgacgagatg gaaatcacgg cgccaaaggt tctctccacc acctggaaaa cgacgcgaaa
      301 tttccgccgc tatcccgatc gccactatga aatcaccgaa ggcgaatgcg agcaggagga
      361 tctgatcccg ggaaaagacc agttcggcaa cgacatattc gtcccgaacg cgcagcaagc
      421 ctatggaacg gtccacacag tcaaataggg atgatcccag atgccgcgga aggtcgttgt
      481 tctcgctgct gcgctgatgc tgtgcttcgc aggtgccgcg ccggcgcacc attccaacgc
      541 cgcctacgat cttgaacacc agcagaccgt ggaagccacc gtcaagcagg tcaactggac
      601 caacccccac atctctttcg tggtcgagac cgacgccaag gacggcaagc cgaccgaaac
      661 ctgggtgttc gaagtgtcct cgccgggggt tctgacgcgc tcgggctgga acaagcgttc
      721 gctgcaggtc ggcgatcacg cactcttcca gtatgcgccg cttcgggacg gcaggcccgg
      781 cggatttttg cgaaaagtga cgctgccaag cggccaggaa ctgacctaca gcctgcagcc
      841 agaggaacaa tgatccgatc atgactgcgg tgctgagcgc gctttcgaag tggctggctg
      901 cgacttcgct cagccatacc atccagtccg tcggctggat cattccaacg cttcagacca
      961 ttcacatcct ctgcgtggcc gtggtgtttt cctccgcagt gctggtcgat tttcggatct
     1021 atcggctgtt cgagcgcgac gtgccgctgg cggagatttc gcggcgattt ctccgcgcga
     1081 tctggccggt gctgctggtt ttgctcgtga ccggaagtct gctgatcatc ggcgagccgc
     1141 gccgttcgtt gctcaattcc accttttata tcaaaatgag cttgctcctg gccgcggtgc
     1201 tgctgaccgc cgctttgcag ggctcaatcg cggcctcgcc gggcttttgg gaaaaggatc
     1261 gcggccgacg gatggcaggc aaggctctcg cgacgctgtc gatcgtgatc tggtgcggca
     1321 tcctgttcgc cggccgctgg attgcctaca cgcaggccgg atgatgtctc tatttcttga
     1381 cgtcattggc tatttcgaga acagcaccgt tgccgacgcc atccgcgaaa acgacgtgct
     1441 gttcccgaca atcgaatccg ttcatgttgt cgcgatctgc ctcgtggtcg gctcaattct
     1501 cgtgctggac ctgagactgc tcggcttcgc ctcgctccaa cggccggtga gccggcttgc
     1561 gggtgcgatc ctgccgataa cctggggcgc gttcacgctg gctgcggttt ccggctttct
     1621 gcttttcatg tccaacgcga caaaatatct tgcgaacggt tactttgtcg caaagatgtt
     1681 tttgatctgc gccgcaggcg caaacatgat tatcttccac ttgatcagtg cgcgcgatct
     1741 gccgcaatgg gagaaggacg cctccccgcc actgctcgtg cgaacggcag gcgcgctctc
     1801 cattctgctc tggatctcgg tcgtcgcctg cgggcgctgg atcgggttca ccatgcggcc
     1861 gctctgagat gggcagcctc tcccgcccct caggctttgc ggccgcgatc gtcgtcgcaa
     1921 gcctggccgc atcgccatcg ctgtccgagc cgcccccgac ggcgcctttc cgggcgcatg
     1981 tcgacatgaa ggccttcatg gagcacgtcc ttcagcctgc cgcgagcctg atctggaccg
     2041 tgaacggcgt gatgatcgac gaaaagggcg agcacgacct cggcccgaaa agcgacgacg
     2101 attgggagaa gcttgtcagc gccgcggcca cgctcgccga ggcaaccaac gcgctgatga
     2161 tcccgcagcg ggcgctcgat cctgactgga accgctatgt cgaaagactc gccgatgcgg
     2221 ccgacaaggc ctaccgcgcc gcggaggcgc atgacctgaa atccatttcg gaggtcagcg
     2281 accgcctcga cggcatttgc gcggcctgcc accgccatta cggcgtcgag tagaggcgcc
     2341 ggcgacgccg taatcacccg ctttcgcggg tggcggcgcc agcggactga aaacggcccg
     2401 gctcaattcg ccgggatgta tttgccgtcc ttcaccgtga ggatgatccg cgagcggtcg
     2461 tcgagaccgt agcggtcctt ctcggtgaaa ttgtagacgc cctggcttgc gggaatttcg
     2521 cgctcggtga ggaaggcctg gcggatcgcc tcgcgaaatt cgggcgtgcc gggctttgcg
     2581 gtcttcagcg cagtcggaat gacgcgcttg aggacttcaa aggcgtcgta ggaatggccg
     2641 gcgaactggc tgcggctgtt cgggccatac ttcgcctcat aggcggtgtc gagcgcaagt
     2701 cccggctttt tggtgagcgc cgtatcgggc tgcgtctccg gagacatcac cgggcccgaa
     2761 gccatgatca cgccttcggc cgctttgcct gcaatacgga tgaaatccat gctggcggcg
     2821 ccatgggtct gatagatcag gcccttgtaa ccgcggtcgc gcagttcggt ctgcggcagc
     2881 gccgcggcag tgccggacgc gccaaccaga atggcgtcgg gattggccgc gatcagcttc
     2941 aatacctgtc cggtgaccga ggtgtcaggc cgcgcaaagc gctcttcgtc gacgacggtc
     3001 atccccatcg gcacgccctg gttcttgaag tcgttgaacc agagatcgcc gtaggaatcg
     3061 gaatagccga tatagccgac ggtcttgatg ttgtgtgcct tcatgtgctc gtagagcacc
     3121 ttgcccatga tcggcaccgg ctgcggcatg tcgaccgacc acttcccgcg cgcctccgtg
     3181 atcggaaagg gcgcgagccc gatatgcgga atgccggcct cattggcgac gttggacacc
     3241 gcgatggtcg gcggcgtggt cgaagagccc atgatgatgt cggccttcga ttccgtgaca
     3301 aagcggcgcg cattggtggt ggcgttggtc ggatcgccgc cgtcgtcgag cacgatcacc
     3361 ttcagcggga tgcctgcgat ctccttcggc acgaattcca gcgcattgcg ctcggggatg
     3421 ccgagcgccg ccgccggccc cgtggtg
//
LOCUS       DATLJT010000494         9852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3475520,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000494 DATLJT010000000
VERSION     DATLJT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475520"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VM910_11390"
     CDS             <1..225
                     /locus_tag="VM910_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63170.1"
                     /translation="GAFFYPRRQLLANAAIASSRVGASEERQRWERRVREAQSAVKAL
                     AESVPKAVEGEKPKLLPGSELAPHSVGCGE"
     gene            complement(236..1768)
                     /locus_tag="VM910_11395"
     CDS             complement(236..1768)
                     /locus_tag="VM910_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63171.1"
                     /translation="MAREHLIQRGHQYYLRLAIPRPIRHLFPSGTGKPRDFISEPLGR
                     NYDPARVEADRRVADYRALFARAGLMTAEAVQGEIQAIRRQAEGRQTVADLPTLQSEL
                     ERVRREYRERVDRVAAGPPYDAAVSPAIDAFVAAMKAEARRDIAKEVGRLVKDTDIAE
                     GSEAWNDLADRVMKEQSRAWKESIADRVNQKPVPTVPERETITQAAEAWFAEMQRTDV
                     RPQTLDGHRLRIRSFVERCGDVALTSVTRTMASDFLTAMASGRSNRTVNNYAMTLMCL
                     FKHARNRGRFTGENPFEDQRRKVASEKREAFTDEEIAKLFAALPAERAPKRHSPETAL
                     PWAVRIAAYTGMRLEEIAQLTVADVQTRGSNGGTVIVFDVHNGDDAHHLKNDGSARAV
                     PVHSELVRAGFLHYGQALPQDGLLFPGLTRRESKGGKIGARIGELFRKKLIALEMKRD
                     GLCFHSLRHTVAERLEAAAVSQTDAARVLGHAIAGESYGTYSSGPGLKRLAAVVEEIR
                     YE"
     gene            complement(2363..3067)
                     /locus_tag="VM910_11400"
     CDS             complement(2363..3067)
                     /locus_tag="VM910_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HVI63172.1"
                     /translation="MHSVTVRKEIVDRVLQRRGRYHLFDRLDPDRTALVVIDMQNTFC
                     APGGPAEVPASRGIVGPINRLTAELRSLGVPVIWVLHANSRIGGKSDWELFFNTIVAD
                     DVREKTLESLAAAKQSVWAALVTGPTDQTVLKNRYSALIPGSSPLERMLRGMEIDTIL
                     VAGTKTNVCCESTARDAMMLDFKVVLVEDCSAALSDDEHRSALENIIQQFGDVMTADE
                     VLARLRHSENARGPRS"
     gene            complement(3077..4588)
                     /locus_tag="VM910_11405"
     CDS             complement(3077..4588)
                     /locus_tag="VM910_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVI63173.1"
                     /translation="MTMAMTATEDPVVRTKPGELRGTCESGIAVFRGVPYAAAPVGEL
                     RFAPPQPVPVWRGVRDASLDSPIAPQGRSRLAHIMGDFERPQSEDCLTLNIWTPAPDS
                     AKRPVLVWVHGGAFASGSGSLRWYSGERFAANGDVVVVSINYRLGALGFLCLPGVSDG
                     NLGLLDQVAALRFVRDNIAAFGGDPDNVTVVGQSAGAASIAILMTMPQAVGLFRRAIL
                     QSTPFGRMSRTLEDSHRIGRRLLEVLGLEPDEAGTLKSLPFARFVTAQGEVARLEKKF
                     ADALAPFWPVIDGNVYPGEVAPALKAGAGADLDTMIGTTREEMAAFYCIDQEVANADA
                     SAIEGVFVAMFKSDHRPYYEEIRRMRASGTNAALLGDLMSDALFRIGSLLMAEARADQ
                     RRPAYVYQFDWQSPAGFESCHCLEIPFVFNNFANWTDSPMLKGAKPEETKGLAEAMHG
                     AWIAFARTGKPDHPRMPVWPPYQRGDRMTMRFDTVIGPVSDLAGLAWRQPWLR"
     gene            complement(4598..5338)
                     /locus_tag="VM910_11410"
     CDS             complement(4598..5338)
                     /locus_tag="VM910_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI63174.1"
                     /translation="MVADRVIGEGLVVLALLAWWMLARGLPEFILPGPVGVVRRLIEL
                     FMMPEFLWHAFTSAWRVLVSIAAALLIGGGLAFLSRSVPWLEAVVDERIKPVLNSFPS
                     IGWAILAAIWFEPGDFGVIFVEVAILIPFCLINIAEGLRNIDRELMEMGQSFTRRRLR
                     ILWRLTLPLLVPYGLSATRIAYGIAWKIALVAELLGAPSGLGYLMLRAQTAADSTTFL
                     ATCFAIVLMFVAGERLVIVPLERRFAHR"
     gene            complement(5358..6155)
                     /locus_tag="VM910_11415"
     CDS             complement(5358..6155)
                     /locus_tag="VM910_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI63175.1"
                     /translation="MVDTAIALAQSSRSRGLFARRLAAHLFMLLFLAAWQAAALVAPS
                     YLLPSPVQVAWRLWSFVTSWRDLGHLGASLYHVAAAIAISFVIGSLLALIPYYVPLLR
                     FAIERRIGPFLNSFSAIGWTLLSIMWFGVSPLTVIFAISAVLVPFALVNMSAGLANLD
                     AEILEMAESFTRQLWRVFALVIVPSLYPFIFATLRIMFGVAWKVTLTAELFGGNSGLG
                     YMINLSRQEFDTATIFAAIILIIAFVHSMDHYILAPLQASVSRHYAY"
     gene            complement(6155..7144)
                     /locus_tag="VM910_11420"
     CDS             complement(6155..7144)
                     /locus_tag="VM910_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HVI63176.1"
                     /translation="MIKRRSVLKLMAAPAVMTLARPGFAKEKVTYAYLLDPAYDAVTW
                     AMSNEKTSSDLITVEARGLAIPQLIQATSAKQYDVIMTAVIAIPPAAARGLELRVLST
                     ALQQSSKGEGAGVWVKNDSPFKNPKELKGKSLGSYALRSTGYTQVRLALIKKYGLNAA
                     LEGGDLKQVEIQAPNLPGALAAGQIDAATLIHSQAYRALKSGEFRPIAETGRDNIEAF
                     GMRFISALNVSYPERVAQRPEAFKEFNRMFRDSVRYTLAHRDEVFGAVGKQTNLPPDF
                     FNWWFEKSSDVPGTFEEGHAQIINRFYELSKEIGMIQSYPDIRTVVWEHALRD"
     gene            complement(7068..7925)
                     /locus_tag="VM910_11425"
     CDS             complement(7068..7925)
                     /locus_tag="VM910_11425"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63177.1"
                     /translation="MVTDPIITFENVGVAFGEEQIYDHLSFDVRRGQFVCILGPSGCG
                     KSTLLRMIGGLLEMNMGRVTVDGRTPRDAWPEIAFVFQSPRLVPWRTALDNVLLGMEL
                     RFGSGDKERRRARATELLTLVGLAGDTRKYPSMLSGGERQRVAIARALAVDPKIVLMD
                     EPFSALDPNMRSRMRVEMERIWLETGKTVVFVTHDIDESLQLADRTVVLSNKPTRVLE
                     LIELQTPRPRTLGDRGLDTHRQKLVKLFRSLEQQPTSQRKPSNDQTSLRPQAHGRPGS
                     DDPGPAWIR"
     gene            complement(8144..9049)
                     /locus_tag="VM910_11430"
     CDS             complement(8144..9049)
                     /locus_tag="VM910_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63178.1"
                     /translation="MSAPSHWTFGESTLRRIVEYEGPILSPFEIFGDCTQAHLDQNRA
                     WLVPRFQDATSGILIITIQSFLIRRNGLTVLVDACGGNDKERARPHFHRRSWPWLETL
                     RSAGVAPEDIDVVLCTHLHVDHVGWNTRLESGRWVPTFPNARYLIAQREWDYWRAAGP
                     EALARTGDYITDSVLPIFKSGQAGLIGDEHMLASDIALETAAGHTPGLTLVRLSGGRH
                     EAIVCSDLMHHPLQVRYPTWSTRFCVDPDQARLTRIEFFKKHADSGRLVFPTHFPTPT
                     GGTIARDGADYHFVFDGEVRPAILP"
     gene            complement(9592..>9852)
                     /locus_tag="VM910_11435"
     CDS             complement(9592..>9852)
                     /locus_tag="VM910_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI63179.1"
                     /translation="AECEKGGLAFLPWHPLAAGGDAAGGGALGRVARRRGATPMQVAI
                     AWLLARSRVIVPIPGTSSVAHLDENVAAARILLSTEDLHELS"
BASE COUNT         1965 a         3080 c         3011 g         1796 t
ORIGIN      
        1 ggggctttct tttaccctcg ccgccagctc ttggcgaatg ccgccattgc gtcctcgcgc
       61 gtaggtgcga gcgaagagcg ccagcggtgg gagcgccgcg tgcgtgaggc acagagcgct
      121 gtgaaggcac tcgcggagag cgtgcccaag gccgttgaag gggagaagcc caaattattg
      181 ccgggcagcg agctggcgcc gcacagcgta ggatgcgggg agtgagctta agcccctatt
      241 cgtatcgaat ctcctcgaca acggccgcaa gacgcttcag gcccgggccg ctggaatagg
      301 tgccgtagct ctcccccgca atggcgtgcc cgaggacgcg cgcggcatcc gtctgcgaga
      361 ctgcggcggc ctctagacgc tcggcgacgg tgtgtcggag ggagtgaaag cacagcccat
      421 cacgcttcat ctcaagcgca atgagcttct tgcggaacag ctcgccgatc cgtgccccga
      481 tcttgccgcc cttgctctcg cggcgcgtca gcccggggaa cagtagcccg tcctgcggta
      541 gcgcctgacc atagtggagg aagcccgcac ggacgagttc gctgtgaacg ggaacggcgc
      601 gcgcgctgcc atcgttcttc aggtggtgag catcgtcgcc gttgtgaacg tcgaacacga
      661 tcaccgtccc accattcgag ccacgcgttt ggacatccgc aacggttagc tgcgcgattt
      721 cttcgaggcg cattccggta taggccgcga tccgaaccgc ccacggcagc gccgtctcgg
      781 gtgagtgcct cttcggtgcg cgctcagcgg ggagtgcggc gaacagcttg gcgatttcct
      841 cgtcggtgaa cgcctcgcgc ttctcgctgg ccaccttgcg gcgctgatcc tcgaatgggt
      901 tctcgccggt aaagcgtccg cgattgcgag cgtgcttgaa caggcacatc agcgtcatgg
      961 cgtagttatt aaccgtgcgg ttagagcgcc cgctcgccat ggccgtcagg aagtcgctgg
     1021 ccatcgtgcg ggtgactgac gtcaacgcga catcgccaca gcgctccaca aagctccgta
     1081 tgcgcaagcg gtggccgtcc aatgtctggg ggcgcacatc ggtgcgctgc atttcggcaa
     1141 accaagcctc agcggcttgg gtgatggttt cgcgctcggg gacagtcggg actggctttt
     1201 ggttcacacg atccgcaatg ctctctttcc aggcgcgaga ttgttccttc attaccctgt
     1261 ccgcgagatc attccatgcc tccgaaccct ccgcgatatc ggtatccttc acaaggcgcc
     1321 caacctcttt cgcgatatca cggcgcgcct cggctttcat ggccgcgacg aaggcatcaa
     1381 tggcgggcga cacggccgcg tcatagggcg gccccgccgc tacgcgatct acccgctctc
     1441 gatactctcg ccgcactcgc tctagttcgc tctggagcgt gggcaagtcg gcgaccgtct
     1501 ggcgcccctc tgcctgccgc cttattgcct gtatttcgcc ttgtacggct tcagcggtca
     1561 ttagaccggc gcgggcgaat agcgcgcgat aatcagccac gcggcgatca gcctcgactc
     1621 tcgcgggatc atagttgcgc cctagcggct cagagatgaa atcgcgtggc ttgccggtgc
     1681 cgctcggaaa caggtggcga atgggacgag ggatcgcgag ccgcagatag tattggtgcc
     1741 cccgttggat cagatgttcc cgcgccattt ggccctcctg tagcacagcg tgtagtacag
     1801 aagatatgcc cgtaaaaatg ccccgtcaat ggggaatata gcaaaaatgg gcacaaattg
     1861 cgggtcagcc taccggcatc gccggctgct gggcgtgcgc cgtgagcggc catgccgcca
     1921 cgccgccgag gaaggtgatg aattcccgtc gcttcaattg atcgaattgc attcggtgcc
     1981 ctgccaacgt ccccagccag ttggaactgt gccagcgcta gacgcaaggc cgcgctcatg
     2041 catgccagga tgagagcggc gcttgctgac cttgatgagg gcgagttcac cgaaggccac
     2101 gagcttgatc gcaagtggcg agaccggctc catccacatg gccgcttgat ttcaataggc
     2161 cggagactcg gttcgaattc atatgagatg ggttcgatat tccattcgat cgccttggcg
     2221 atgcttgctg cctcgtcgag ccattgccgg attccaaacc ctgcggatgc tccgccaact
     2281 ggcggcggat ccatgtggca cggagaactt tgggacggtc ggcaacggat cgtcggggct
     2341 ttgttggcgc agcatttcag ggttacgagc gcggccctcg cgcgttttca gagtgtcgaa
     2401 gccgggccaa cacctcgtcg gccgtcatga catcaccgaa ttgctggatg atgttttcca
     2461 gagcgctgcg atgttcgtca tccgatagcg cggcagaaca atcttcgacc agaacaacct
     2521 tgaaatcgag catcatggcg tcgcgggccg ttgattcgca gcatacgttc gtcttggtgc
     2581 ctgcgacgag gatggtatcg atctccatac cgcgaagcat acgctcgagc ggcgacgagc
     2641 ccgggatcaa ggcactgtag cggttcttca gcactgtttg gtcggtcggc ccggtgacaa
     2701 gcgcggccca gacgctttgc ttcgcagcag ccagactctc aagcgtcttt tccctcacgt
     2761 cgtcagcgac gatggtgttg aagaaaagct cccaatcgct cttgccgccg atccggctgt
     2821 tggcatgcag gacccagatc acgggcacac ccaaactgcg gagctcggcc gtcagtctgt
     2881 tgatcggtcc aacgatgcct ctcgatgccg gcacctcggc aggaccgcct ggagcgcaga
     2941 acgtgttctg catgtcgatt acgaccagcg ccgtgcggtc cgggtccagc cgatcgaaca
     3001 gatggtaacg gccgcggcgt tgtaggacgc gatcgacaat ttccttgcgg accgtgacgg
     3061 aatgcatgag tccatctcag cggagccagg gctgacgcca tgccagcccg gcgagatcac
     3121 tgaccggccc gatcacggtg tcaaagcgca tggtcatgcg gtccccgcgc tgatacggcg
     3181 gccacaccgg catgcgcggg tgatccggtt ttccggtgcg ggcgaatgcg atccatgcgc
     3241 catgcatcgc ctccgcaagg cccttggttt cctcaggctt ggcgcccttg agcatcggcg
     3301 agtccgtcca gttcgcaaaa ttgttaaaca cgaatggaat ctcgaggcag tgacatgact
     3361 caaatccggc cggtgattgc cagtcgaact ggtacacata agcaggccgt ctttgatcgg
     3421 cccgcgcttc ggccatgagc agactgccga tccgaaacag cgcatcgctc atcagatcgc
     3481 ccagcagcgc tgcgttggtg cccgaggccc gcatccggcg gatctcttcg tagtaaggcc
     3541 gatgatcgga tttgaacatc gctacaaata cgccttcgat cgcgctagcg tctgcgttcg
     3601 cgacctcctg gtcaatgcag tagaacgccg ccatctcctc acgtgtcgtg ccgatcatgg
     3661 tgtcgaggtc ggcgccggcg ccggccttga gcgccggcgc gacctcaccg ggatagacgt
     3721 tgccgtctat caccggccag aacggcgcaa gcgcatcggc gaatttcttt tcgaggcggg
     3781 cgacctcgcc ctgcgcagtc acgaatcgtg cgaacggcag cgatttgagc gtccccgctt
     3841 cgtccggctc aagtcccagt acctccaaca aacgccgccc gatcctatgg gagtcttcga
     3901 gcgtgcgcga catgcgtcca aacggcgtgc tttgcaggat cgcgcgccga aacagaccga
     3961 cggcctgcgg catagtcatg aggatcgcga tcgaggccgc tcccgccgac tgcccaacta
     4021 ccgtcacgtt gtccggatcg ccgccaaagg cggcaatatt gtcgcgcacg aaacgcagcg
     4081 ccgcgacctg atcgagcagt ccaagattgc cgtcactcac tccaggcagg cacagaaacc
     4141 ccagcgcacc aagccggtag ttgatcgaaa ccacgaccac gtcgccgttg gcggcaaacc
     4201 gctcgccgga ataccaccgt agtgaacccg atccgctggc gaaagcacca ccgtggaccc
     4261 acactagaac tggacgtttt gcagaatcag gggcaggcgt ccagatattg agcgtgaggc
     4321 agtcctctga ttgcgggcgc tcgaaatcgc ccatgatgtg ggcaagccgc gaacgcccct
     4381 gcggcgcgat cgggctgtcc aggctcgcat cgcgaacgcc ccgccacacc ggcaccggct
     4441 gcggcggggc gaaccgcaac tcgccgacgg gagccgccgc gtaaggaacg ccgcggaaga
     4501 ccgcaatgcc gctttcgcaa gtgccacgca actcaccggg cttggtccgc accactgggt
     4561 cttcagtcgc agtcatcgcc atggtcatgc cccgtcttca ccggtgcgcg aagcgccgct
     4621 ccagcggcac tatcaccagc cgctcgccag caacgaacat cagcacgatg gcaaagcagg
     4681 tggcaaggaa ggttgtgctg tcggccgcgg tctgggcccg cagcatcaga tagccgagcc
     4741 cactcggagc accgagcaat tctgcgacca acgcgatctt ccaggcgatc ccgtaggcta
     4801 tacgggtcgc ggagagccca tatggcacca gcagcggcaa ggtcagacgc cagagaatgc
     4861 gcaaccggcg gcgagtgaag ctctgaccca tctccatgag ttcgcgatca atattacgta
     4921 gtccctccgc gatattgatc aaacagaacg ggatcaggat cgcgacctcg acgaagatga
     4981 cgccgaaatc accgggctcg aaccagatcg ccgccaggat cgcccagcct atcgagggaa
     5041 acgagttgag caccggcttg attcgttcat cgaccacagc ctcgagccaa ggaacgctac
     5101 gggagagaaa tgcgagcccg ccgccaatca gaagagccgc cgcaatcgag acaagcaccc
     5161 gccaagcgga cgtgaacgcg tgccatagga attccggcat catgaacaat tcgatcagcc
     5221 gacgcacgac cccgaccggc ccgggcagga tgaattccgg caagccgcgc gccaacatcc
     5281 accaagccag cagcgcgagc acgacgagac cctcgccgat cacgcgatcg gcaaccaccg
     5341 acgtgactga tgttcgctca gtaagcatag tgacgcgata cgctcgcttg cagcggggca
     5401 agaatgtaat ggtccatgct gtgcacgaat gcgataatga gaatgatcgc cgcaaagatc
     5461 gttgccgtat cgaactcctg ccgtgacaga ttgatcatgt agccgagccc gctgttgccg
     5521 ccgaagagtt cggccgtcag cgtgaccttc caggcgactc cgaacatgat gcgcagcgtg
     5581 gcgaagataa acggatagag ggacggcacg atcacgagcg caaacacccg ccacagctgg
     5641 cgcgtgaagc tctcagccat ttcgaggatc tcggcatcga gattggcgag gcctgccgac
     5701 atgttgacca atgcgaatgg caccagcacg gcgctgatcg caaagatcac ggtgagcggc
     5761 gaaaccccga accacatgat cgatagcagt gtccagccga tggccgagaa cgaattcagg
     5821 aacggaccga tgcggcgctc gatcgcgaac cgcagcagcg gcacgtaata cgggatcaac
     5881 gcgagcagtg agccgatgac gaacgaaatc gcgattgccg cggccacgtg gtagagcgac
     5941 gcgccgagat ggccaagatc gcgccagctc gtgacgaagg accagagccg ccatgcgacc
     6001 tggacaggac ttggcagcag ataagacggc gcgaccaacg ccgccgcttg ccaggcggcc
     6061 aggaacagta gcatgaaaag atgcgcggca agtctgcgtg cgaataggcc gcgcgaacgc
     6121 gaagattggg cgagcgcgat ggcggtatcg accatcagtc gcgcaacgcg tgctcccaaa
     6181 cgacggtgcg gatatcggga tagctctgga tcatcccgat ctctttcgac agttcgtaga
     6241 acctattgat gatctgggca tggccttcct cgaacgtgcc aggcacgtcg gagctcttct
     6301 cgaaccacca gttaaagaag tccggcggca ggttggtttg cttgccgacc gcgccgaaca
     6361 cctcgtcgcg gtgcgcaagc gtgtagcgca cggagtcacg gaacatgcgg ttgaattcct
     6421 tgaaagcctc gggccgctgc gccacccgtt ccggatatga cacgttgagc gcgctgatga
     6481 agcgcatgcc gaaggcctcg atgttatcgc gtcccgtctc ggcgattggg cggaactcgc
     6541 cggatttcag ggcgcgatag gcctgactgt ggatcagcgt cgcggcgtcg atctgaccgg
     6601 ccgccagagc gccgggcagg ttcggcgcct ggatctcgac ttgcttgaga tcgccgcctt
     6661 caagggcggc attgagtccg tatttcttga tcagcgccag acgcacctgc gtgtagccgg
     6721 ttgagcgcag cgcataggag ccgagcgact tgcccttgag ctcctttggg ttcttgaaag
     6781 gagaatcgtt cttgacccag acgccggcgc cttcaccctt ggatgactgc tgcagcgccg
     6841 tcgacaacac cctcagctcg agaccgcgcg ccgccgccgg cgggatcgcg atcacggcgg
     6901 tcatgatcac gtcatactgc ttggctgagg tggcctggat cagctgcggg atcgcgagcc
     6961 cacgcgcctc gaccgtgatc agatcggagc tggtcttctc gttggacatc gcccaggtga
     7021 ccgcgtcata ggcgggatcg agcagatagg cgtaggtgac cttttcctta gcgaatccag
     7081 gccgggccag ggtcatcact gccggggcgg ccatgagctt gaggacggag cgacgtttga
     7141 tcattcgacg gcttcctttg tgatgtgggc tgctgctcca gcgatcgaaa cagcttaacc
     7201 aatttctggc gatgtgtatc gagaccgcga tcgcccagcg tgcgtggccg tggcgtctgc
     7261 aactcgatga gctcgaggac gcgggtcggc ttattcgaca gcaccaccgt gcgatcggcc
     7321 aattgcagag actcgtcgat gtcgtgcgtg acgaacacca cagtcttgcc ggtctcgagc
     7381 cagatccgct ccatttcgac ccgcatgcgc gagcgcatgt tagggtcgag cgccgaaaat
     7441 ggctcgtcca tcagcacgat cttgggatcg accgcgagcg cacgcgcgat cgcgacccgc
     7501 tggcgctcgc cacccgagag catcgacggg tatttgcgcg tgtctccggc gaggcccacg
     7561 agggttagca gttcggttgc gcgcgcccgg cgtcgctcct tgtcgcccga gccgaaacgc
     7621 agttccatgc ccagcagcac attatcgagt gcggtgcgcc agggaaccag ccgcggtgac
     7681 tgaaacacga acgcgatttc cggccaggcg tcgcgtggcg tgcgcccatc gacggtgacg
     7741 cgccccatat tcatctcgag caggccgcca atcatccgca acaaggtgga tttgccgcaa
     7801 cccgaagggc caaggatgca gacgaactgg ccgcggcgga cgtcgaagct caggtggtcg
     7861 taaatttgct cttcgccaaa agcgacaccg acattctcaa aggtgatgat cgggtcggtt
     7921 accatacgga tgtcgacctt ttgctcctgc tatcatgccg ttcagtgcgt gctttcccgc
     7981 tggcggaata ggccggttct cccgatgaac ctcgacctga tatcgaggat aaagagcttc
     8041 ccgatccctg caaagcccac tgctttgctc cattggttgg agccgagcac ggctcgtcgt
     8101 gggacgggga atgtctgatg acatttttga ggttcgaatc cttttacggc agaatagcgg
     8161 ggcgaacctc gccatcgaat acgaaatgat agtcggctcc atcgcgcgca atcgtgccgc
     8221 cggtcggcgt aggaaaatgc gtagggaaaa caagccgccc gctatcagca tgcttcttga
     8281 agaactcgat acgcgtcagc cgcgcctgat caggatccac acaaaagcgc gtgctccatg
     8341 tcggatagcg tacctgcagc gggtgatgca tcaggtcgga acacacgatc gcttcgtgtc
     8401 gcccgccgct gagccgcacg agcgtgagcc cgggcgtgtg gcctgccgct gtctccaacg
     8461 cgatatcgct cgctagcatg tgctcgtcgc cgatcaggcc cgcctgtccg gacttgaaga
     8521 tgggcaaaac gctatcggtg atgtagtcgc cggtccgcgc gagcgcttca ggtccggccg
     8581 cacgccaata atcccattca cgttgtgcaa tcaggtaccg cgcattggga aaggtcggca
     8641 cccaacggcc gctttctagc cgcgtgttcc agcccacatg atcgacatgc aggtgcgtgc
     8701 acaagaccac gtcgatgtcc tcaggcgcga cacccgcgga tcgcagagtt tcgagccacg
     8761 gccagctgcg acggtgaaag tgagggcgtg cgcgctcctt gtcgttgccg ccacaggcat
     8821 ccaccaggac ggtaaggcca ttgcgccgga tcaagaagct ttggatggtg ataatcagaa
     8881 ttccgctcgt cgcgtcctga aaacgcggca ccagccaagc acgattctga tcgagatgcg
     8941 cttgcgtgca gtcgccaaaa atctcgaacg gcgacaagat cgggccttca tactcgacga
     9001 tgcggcgcag cgtcgattcg ccaaatgtcc agtgcgatgg ggctgacatc gagcgccgac
     9061 catatcaatc tcgaccgtgg cagtcgacgc cgctcctggc acgaagggac atccactttc
     9121 tacccaagaa atccgctatc cgagggaagc cgacgtcgtg cgcgtcgaca gggctcaaat
     9181 atctgaaaat tcgaagcgga gaccggttcg aattctgtca tccaccagcc agacagtgcg
     9241 gtctctgtgc gcatcgcgct agcggcacga tcaagcttgc tgattgaccg ctttcgcgcg
     9301 cgccgtcgcg gcccttgatt ttgcttgtcg tttcgggtgg tggcgcgcgc ctccatccga
     9361 ccatggcctc gcctccgcga attcattgcg gcgccgtcgg cacgtgccca cgcaggacat
     9421 ttggcgcgct gcttcgccga acaccctaca ggcggcgagg aagcctatgc ccgcataatt
     9481 aaatgtgggc ggaacagctt tagttccgga gcgactagcg tcctcagagc cgtgtttgcc
     9541 agccgagatg aaccaatggc ttcggcaccc gcgttgccag aaggaaccgt ttcaggacag
     9601 ttcgtgcaga tcttcggtcg aaaggagaat ccgcgcggcg gcgacgttct cgtcaaggtg
     9661 cgccaccgat gatgtcccgg gaatgggaac aatgacgcgc gagcgggcta gcagccaagc
     9721 gattgccact tgcatgggcg ttgcgcctcg gcggcgcgcg acgcggccaa gcgcaccacc
     9781 ccccgccgca tcgccgccag cagccagggg atgccaaggc aggaaggcaa gtccgccttt
     9841 ctcacactca gc
//
LOCUS       DATLJT010000495         1780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3015869,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000495 DATLJT010000000
VERSION     DATLJT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015869"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VM910_11440"
     CDS             complement(<1..273)
                     /locus_tag="VM910_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI63180.1"
                     /translation="MNRSYAEMAAHYGTAIVPARPYRPRDKAKVEVAVLLATRWIIAK
                     LRNRRFFSIAELNDAIRDCVTTLNERVTRHLGASRRMLFEDIERPML"
     gene            complement(410..1423)
                     /locus_tag="VM910_11445"
     CDS             complement(410..1423)
                     /locus_tag="VM910_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63181.1"
                     /translation="MTAIGPIVYAFFEQHLKAEKGLSPASVRSYRDALRLFLSFVAAE
                     RGRSLTRITVEDFTAERVRHFLGHLETCRGNHIRSRNQRLAGLHTFFNYLARQAPEAL
                     AEAERVAAIPRKRAPPPPTQFLERDEIETLIAGLPATGHAALRDRTLLLFLYNTGARV
                     QEAADLRMGNLELGPQPRVNLHGKGDKWRACPLWSETARLLRQLLAERAAAAPPTAPV
                     FTSRQGRPLTRYGIYKLVRRHAAHLTASANGGPPRRVSPHVLRHTTAVHLLEAGVEIN
                     VIRGWLGHVSLDTTNRYAEINMRSKEAALEACEAPVAASAAFPRRPVWRDDAALLNWL
                     TSL"
     gene            complement(1420..>1780)
                     /locus_tag="VM910_11450"
     CDS             complement(1420..>1780)
                     /locus_tag="VM910_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HVI63182.1"
                     /translation="GTLPPEASAFSFTSGRSIHPGTISQTFHALVPRLGLTLSAGVAP
                     PRVHDLRHSFAVGTLLRWYRQGSDPAARLLHLSTFLGHVDPASTAVYLTITVELLEAA
                     GDRFGRYAAPLSTGATP"
BASE COUNT          320 a          537 c          604 g          319 t
ORIGIN      
        1 gagcatcggc cgctcgatat cttcgaacaa catgcgtcgg ctcgcgccca gatggcgcgt
       61 cacgcgctcg ttgagtgttg tgacgcagtc acggatcgcg tcgttcaact cggcgatcga
      121 gaagaaccgg cgattgcgca gtttggcgat gatccagcgc gtggccagca gcacggccac
      181 ctcgaccttg gctttgtcgc gcggcctgta tgggcgcgcc ggaacgatcg cggtgccgta
      241 gtgggcggcc atctcggcgt agctgcggtt cacctccgga tcgtagaaac acgccttggt
      301 gatgccggac ttgaggttgt ccgacttatg tgccggccga cattatgtgg ccgatcgccg
      361 agttggccca caatcgcggg gttcgacggg gttttcggcc acataatctt cagagcgaag
      421 tgagccagtt cagcaatgca gcgtcgtccc gccatactgg tcttcggggg aatgccgccg
      481 aagctgccac aggggcttcg caagcctcca gcgctgcctc cttcgaacgc atgttgatct
      541 cggcatagcg attggtcgtg tcgaggctga cgtgaccgag ccatccgcgt atgacgttga
      601 tctcgacgcc ggcctcgagc agatgcacgg cggtcgtgtg gcggaggaca tgcggagaga
      661 cccgtcgcgg cgggccacca ttcgcagatg ccgtcaggtg cgccgcatgc cgacgaacaa
      721 gcttgtaaat cccgtatcgg gtgagcggcc gaccttgtcg ggatgtgaat accggagcag
      781 ttggtggtgc ggcggctgcc cgctcggcga gcagttgacg cagcaaccgc gccgtctctg
      841 accacaaggg acaggctcgc cacttgtcgc ccttgccgtg cagattgacg cgcggctggg
      901 gcccaagctc caagttcccc atcctgagat cagccgcttc ctggacacga gcaccggtgt
      961 tgtacaggaa caggaggagg gtgcggtcgc gcagcgccgc gtgtcctgtt gccggcaggc
     1021 ctgcgatgag cgtctcgatc tcatcgcgct cgaggaactg ggttggcggc ggtggcgccc
     1081 ttttcctggg aatcgccgcg acgcgctccg cctcggcaag cgcttccggc gcttggcgcg
     1141 ccagatagtt gaagaacgta tgaagcccag cgaggcgctg gttgcgcgag cggatgtgat
     1201 tgccgcggca cgtctccagg tgaccaagaa aatggcggac ccgctccgct gtgaagtcct
     1261 ccacggtgat tcgggtcagt gatcgaccac gttccgccgc gacgaaggaa aggaacagcc
     1321 ggagcgcatc gcgatagctc cttacggatg ccgggctcag acccttctca gccttcaagt
     1381 gctgttcgaa gaacgcatag acaattgggc cgatggcggt catggcgtcg ctcccgtcga
     1441 cagcggcgct gcgtaccgac cgaaccgatc gccggcagcc tccagcagtt ctacggtgat
     1501 ggtgagatag accgcagtcg aagccgggtc cacgtgaccg aggaaggtgg acagatggag
     1561 aagccgtgcg gccgggtcac tgccttgccg ataccagcgc agcagtgtgc cgacggcgaa
     1621 gctgtgccgt agatcgtgca cgcgcggcgg cgccacacca gccgataagg tgaggccgag
     1681 acgtggaacc aatgcgtgga acgtctggct gatcgtgccg gggtggatcg atcgtcccga
     1741 ggtgaacgag aaagcggacg cctcgggcgg caacgttccc
//
LOCUS       DATLJT010000496         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4165199,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000496 DATLJT010000000
VERSION     DATLJT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4165199"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            656..2350
                     /locus_tag="VM910_11455"
     CDS             656..2350
                     /locus_tag="VM910_11455"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVI63183.1"
                     /translation="MTVTLPQTSTILASLGQAAFAWDIATDAISWSDHVSAVFADISP
                     AALASGAEFAKLIEPHCSIRSDALLLSPPVHDADGVPYRVEYGVRASTSAPMLWIEET
                     GCWFAGPDGRPARAQGIVRINNERRARDEQLIKLSQHDPLTGELNRTHLVAALAEAIE
                     EAQRYRGTSAFMLIGIDHLARINDAFGFDVADAVISEVAKRIRVRLRGGDVLGRFSGN
                     KFGLILKNCTVDDMNIAAERFLAGVRDEVVPTRSGPVSVTVSIGAVSVPRYARSADEA
                     INRAHETLDAAKRRRAGSFLAWRPNVERDAQRRVNIRVTDEIVTALNERRIALAYEPV
                     VTAGSRDAAFYECLVRMQRDGGRVLLAPDIVPVAERLGLIRLVDHRVLELAVAELANS
                     PGVSLSLNLSPDTTMDPDWWSSIESLMHAHPTAAARLIVEITETVAIRDIDDLRGFVT
                     RLKNLGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNIARSADDRAFVQTLIDL
                     ARRLNIETVAEWVQDEESARLLCDWGCDYVQGRLIGLASATRPWVAPQEKMLPATG"
     gene            complement(2410..3003)
                     /gene="phaR"
                     /locus_tag="VM910_11460"
     CDS             complement(2410..3003)
                     /gene="phaR"
                     /locus_tag="VM910_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HVI63184.1"
                     /translation="MAKSDQPTTIKKYANRRLYNTGTSTYVTLEDLAAMVKEGEDFLV
                     YDAKTGDDITRSVLAQIIFEQENKAGQNLLPTTFLRQLIRFYGDSMQMVVPKYLEQSI
                     DTLTREQEKFRKQLASTLSGTPFAPLEEQVRRNMELFQQTFSMFKPFGARSGTAEAEK
                     SPDAASSDDDNIDDLRRQMREMQERLDRLSKEPKKEE"
BASE COUNT          541 a          951 c          942 g          615 t
ORIGIN      
        1 gaccgcgcgg attgcccgat tggcctttct gaaatccgcc agcctgtttc cgccttgtat
       61 tagcaaggga catcgtgccg ctccgctgcc gctaattagg cgacgggagc ctaatccaga
      121 tcccgggtgg aactaagcga aatccctgca cggcttgggg actgatctgg ggactatgcc
      181 tgtaaacttg aaaaaatcaa aaaccttcac agcatgttgt acagatctat aggcggacag
      241 cgcgtccgcg ggcgttgcgc gccgcaggct gcgggtttgc ggaagctgtt cggcggtcgc
      301 gatcagatgc gcaacgaaag ttggatcggg acgtgggttc aaaaatttgc gtgcccaata
      361 cggcggcgcc ggcgcgagcg gaaccatcgc aacgcatgcg ggttcgacct cctcgaagac
      421 gccgtcttcc acgacctgtt cgtcaaaacc ggacattgca aaccgtcaca ttctgggaca
      481 cctggtcctg atacggtttg acctcgcaag gagtatgccg ctcccggcct gtccgttcaa
      541 agcgtgattc aaacgtggtt tccaaattgt ttatgaactt cgcctaggct ggatgccgaa
      601 actggcactc cccaagcccg ggaatcacct ggctgtgtcc cgaaacgagg cgatcttgac
      661 agtcacgctt ccgcagacct cgaccatcct ggcatccctt ggccaagcgg cctttgcctg
      721 ggatatcgca accgacgcca taagctggag cgatcatgtc agcgcggtgt ttgcagacat
      781 atcgccggct gcgcttgcga gcggagcgga atttgcaaaa ctgatcgagc cgcattgctc
      841 gatccgcagc gatgcgctcc tgctttcgcc gcccgttcat gacgccgatg gtgtgcccta
      901 cagggtcgaa tatggcgtgc gggcctcgac gtcggcgccg atgctctgga tcgaggagac
      961 cggctgctgg tttgccggac ccgacggtcg gccggcacgc gcccaaggca ttgtccgcat
     1021 caacaatgag cggcgcgccc gcgacgagca gctaatcaag ctgtcgcagc atgacccgct
     1081 gacaggcgaa ctcaaccgca cgcatctggt cgcggcgctc gccgaagcga tcgaggaggc
     1141 ccagcgctac cgcggcacct cggccttcat gctgatcggc atcgatcatc tggcgcgcat
     1201 caatgatgcc ttcggcttcg atgttgccga tgcggtgatt tccgaagtcg ccaaacggat
     1261 ccgcgtccgt ttgcgcggcg gcgacgtact cggccgcttt tccggcaaca agttcggcct
     1321 gatcctgaag aattgcaccg tcgatgacat gaatatagcc gccgagcgtt tcctcgccgg
     1381 cgtccgcgac gaggtggtgc cgacgcgctc aggcccggtc tcggtcaccg tttcgatcgg
     1441 cgccgtcagc gtgccgcgtt acgcgcgctc ggccgacgaa gccatcaacc gcgcccatga
     1501 aacgctcgat gccgccaagc gccggcgcgc cggttcgttt ctggcctggc gaccgaatgt
     1561 cgagcgcgac gcccagcgcc gcgtcaatat ccgcgtcacc gacgagatcg tcaccgcgct
     1621 caacgagcgg cgcatcgcgc ttgcctacga gcccgtggtg accgccggtt cgcgcgatgc
     1681 ggccttctac gaatgcctgg tgcgcatgca gcgggatggc gggcgcgtgc tgcttgcccc
     1741 cgacatcgtg ccggtggccg agcggctcgg cctgatccga ctggtcgatc accgcgtgct
     1801 cgaactcgca gttgccgagc tcgccaattc gccaggtgtc tcgctgagcc tcaacctctc
     1861 gcctgatacc acgatggatc cggactggtg gtcttcgatc gagtcgttga tgcacgcgca
     1921 tccgaccgcg gccgcgcggc tgatcgtcga gatcaccgag acggtcgcga tccgggacat
     1981 cgacgatctg cgcggcttcg tgacgcgcct gaaaaatctc ggcagccgga ttgcgatcga
     2041 cgacttcggc gcgggctaca cctcgtttcg caacctgcgc aagctcggcg tcgacattgt
     2101 gaagatcgat ggcgcctttg tgcagaacat cgcccgctcg gcggatgacc gcgcgttcgt
     2161 gcagacgctg atcgacctgg cgcggcggct caacatcgaa acggtcgccg aatgggtgca
     2221 ggacgaggag tccgcgcggc tgctgtgcga ttggggctgc gattacgtcc agggccggct
     2281 gattggcctt gcttctgcga cgcgtccctg ggtggcgccg caagaaaaga tgctgccggc
     2341 cacggggtag tcaatctcgc tatgctcgat ttcgagcaag ctctccccca aagggagagg
     2401 gtaagagagt tactcttcct ttttcggctc tttcgacaga cgatcgagcc gctcctgcat
     2461 ttctctcatc tgcctgcgca ggtcgtcgat gttgtcgtca tcggaagatg cggcgtccgg
     2521 cgatttctcg gcctccgccg ttccgctgcg ggcgccgaag ggcttgaaca tcgagaatgt
     2581 ctgctggaac agttccatgt tgcggcggac ctgctcctcg agtggcgcga aaggcgtccc
     2641 gctcaaagtg ctcgcaagct gcttgcggaa cttctcctgc tcgcgcgtca gcgtgtcgat
     2701 cgactgctcg aggtacttcg gcacgaccat ttgcatgctg tcgccgtaga agcggatcaa
     2761 ctgcctgagg aacgtggtgg gcaggaggtt ctggccggcc ttgttttcct gctcgaagat
     2821 gatctgcgcc agcaccgagc gggtgatgtc gtcgccggtc ttggcgtcgt agacgaggaa
     2881 gtcctcgccc tccttcacca tcgcggcaag atcctcgagc gtcacatacg tgctggtacc
     2941 ggtattataa agccgccggt ttgcgtattt cttgatagtt gtgggttggt cagatttcgc
     3001 catgggctca cacacaaacc gaggaaggga accgagcggc gttcgccgc
//
LOCUS       DATLJT010000497         3010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3934816,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000497 DATLJT010000000
VERSION     DATLJT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934816"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..794)
                     /locus_tag="VM910_11465"
     CDS             complement(6..794)
                     /locus_tag="VM910_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI63185.1"
                     /translation="MLKLYHNINSVCAQKVRIALEEKGQEAEEHLLTLRGDQNEPAYL
                     KLNPNGVVPTLVHDGAPIVESSLILYYIDEVFPEPPLMPKAPLPRLRVRMYNKLIDEY
                     VHNACTIMTFATAFRPNFLKVPREVWLAEINKAPLKRRVEYKRSVIEHGLDSEFAGDA
                     LAQHRKLLAWMADSLKSGKYLAGDSFSNAECAVIPYILRLELLQLDGLWADYPAIADW
                     WARMRARPSVKAAIFDRMTEKDWAPFKNLAPDPWPKVQELLRAS"
     gene            complement(912..1850)
                     /locus_tag="VM910_11470"
     CDS             complement(912..1850)
                     /locus_tag="VM910_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HVI63186.1"
                     /translation="MKNLIGVLALALGPALAAAAEKPDWAFPVTDKVQPPPRFEANRV
                     RPAPPGSALSITRAKADDMYDIPNWFPNMYPPMPAIVQYGNKERQVRACGSCHLPTGT
                     GHDESAYVAGLPASYFIRQMADWKSGDRKFSATMVTMAKVVTDAEIKDAADYFASLKP
                     RPWIRVVEADAVPKSYIGQGNKRLLHPDGGTEPLGNRIIEVPEDEEVVVYRDPMSGFV
                     AYVPKGSIARGKDLASTGNGKTLPCSICHGRTLQGLGEVPAIAGRHPNYIVRQLWNMQ
                     NAERVGTSAALMQQVVEKLSNDDMLAVAAYVASLTP"
     gene            complement(2287..>3010)
                     /locus_tag="VM910_11475"
     CDS             complement(2287..>3010)
                     /locus_tag="VM910_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63187.1"
                     /translation="PNSNYYLAINTGFPNSFDKANNRNGSLLMIHGDCSSMGCYAMTD
                     EQISEIYSLARDSLLGRPSFQVQAYPFRLTPANLARHRTHPQFAFWKMLKIGNDHFEA
                     AHLEPKVDVCDRRYVFDAQPPPNSPNPLVFNPTGRCPAFVVNPKIGGRALEKQRADEA
                     EYAQLLKDDVPTVPIYSGLDGGMNEVFLARFPVRVTLAQVLPYASYLPQLPPIPWVDD
                     EGSLTSKWFGNCFEGCEVTVGW"
BASE COUNT          527 a          928 c          963 g          592 t
ORIGIN      
        1 tgggtttagc tggctcgcag cagctcctgc accttgggcc agggatcggg cgcgaggttc
       61 ttgaacggcg cccagtcctt ctcggtcatg cgatcgaaga tcgccgcctt gaccgagggc
      121 cgcgcgcgca tgcgcgccca ccagtccgcg atcgcgggat agtccgccca caggccgtcg
      181 agctggagca gctccagccg cagaatgtag ggaatcaccg cgcactcggc gttggagaag
      241 ctgtcgcccg caaggtactt tccgctcttg agcgaatccg ccatccacgc cagcaatttt
      301 cgatgctgcg ccagcgcgtc cccagcgaat tcggaatcga gcccgtgctc gatcacgctg
      361 cgcttatatt cgacgcgccg tttgagcggc gccttgttga tctcagcgag ccacacctcg
      421 cgcggcactt tcaggaaatt ggggcgaaag gcggtggcga aggtcataat ggtgcaggcg
      481 ttgtgcacat attcgtcgat cagcttgttg tacatgcgca cacgcaagcg cggcagcggc
      541 gccttcggca tcagcggcgg ctcgggaaaa acctcgtcga tgtaatagag aatcagcgac
      601 gattccacga tcggcgcccc gtcgtgaacg agcgtcggca ccaccccgtt gggattgagc
      661 ttgagatagg cgggttcgtt ctggtcgccg cgcagcgtga gcaaatgctc ctcggcttcc
      721 tggcccttct cctcgagcgc gatgcgcacc ttctgcgcgc agaccgaatt gatgttgtgg
      781 taaagcttga gcatcttgtc ctcccgttca caccgcaatt gcgctgtatc gcccccgcgc
      841 gtcacgcgcc gcgctctgcg cggtgccgca gctcgctggt tgcggcacgg atcaaattgc
      901 cgtcttgtcc gtcacggcgt cagcgacgca acataggctg cgaccgccag catgtcgtcg
      961 ttggagagct tctcgaccac ctgctgcatc agcgccgccg acgtgccgac gcgctcggcg
     1021 ttctgcatgt tccagagctg gcgaacgatg tagttgggat ggcggcccgc gatggcgggc
     1081 acctcgccta agccctgcaa ggtgcggccg tggcagatgc tgcatggtag cgtcttgccg
     1141 ttgcccgtgc tcgccaggtc cttgcccctg gcgatgctgc ccttcggcac gtaggcgacg
     1201 aagccggaca tgggatcgcg gtagacaacc acctcctcgt cttccggcac ttcgatgatg
     1261 cggttgccca acggctcggt gccgccgtcc ggatgaagaa gccgcttgtt gccctggccg
     1321 atgtaggact tgggcacggc gtcggcctcg accacgcgga tccagggccg cggcttgagc
     1381 gaggcaaaat agtcggcggc gtccttgatc tcagcgtccg tgacgacctt ggccatcgtc
     1441 accatggtgg cgctgaattt gcggtcgccg ctcttccagt ccgccatctg gcgaatgaaa
     1501 taggacgccg gaaggccagc cacgtaggcg gattcatcgt ggcccgtgcc ggtcggcaga
     1561 tgacaagacc cgcaagcacg cacctgccgc tctttgttgc cgtattgaac gatcgcgggc
     1621 atcggcgggt acatgttggg gaaccagttt gggatatcgt acatgtcgtc cgccttggcg
     1681 cgcgtgatcg agagcgcgct gccgggcggc gcgggtcgca ccctgtttgc ctcgaagcgg
     1741 ggaggaggtt gaaccttgtc tgtgaccggg aacgcccaat ccggcttttc cgccgcggcc
     1801 gcgagcgcag gcccgagcgc cagcgccagg acgccgatga ggttcttcat gctatcgccc
     1861 caacctcgtt gtcgttcggg agaggtatgc tttcacggcg cgttcgatag tcaagtaggt
     1921 catgcccgat gcaacggcgc gagcagggcg gtgttgccgc gcgtccttct cgctcgcgca
     1981 acgcacgccg cagaggggtt gagcgggtga acgagccgga atcgggaatc gcggtcgccc
     2041 tgcacaccgc tgacccgggt gacagcggcg ccatgcgacg tcagggtctg cttgcactaa
     2101 ctatgcgcgg cagaacgtag cgcggggcag ggaacgcgcc tatggctcat ccgggctact
     2161 tgctgttcgg cggcgcggcc actggttcaa cgttgctgcg tcctcaggcc agcgtcgagc
     2221 gaatcacacg cggccacgac gccgcgccca ttgtttctta cggttgccat tggaccgtac
     2281 tcggaatcac cagcctacag tgacctcgca gccttcaaaa caattgccaa accatttact
     2341 cgtcaacgaa ccttcgtcgt cgacccaggg gatcggcggc agttgcggca ggtaagatgc
     2401 atacggcagc acttgcgcca gcgtcactct gaccggaaac cgtgcgagga aaacctcatt
     2461 cattccgccg tccagtccgc tgtagattgg gacggttggc acgtcgtctt tgagcaattg
     2521 cgcgtattcg gcctcgtcgg cgcgttgttt ctccagcgcc cgccccccaa ttttcggatt
     2581 cacgacaaag gcggggcatc taccggtggg attgaacacg agaggattcg gcgaattcgg
     2641 gggaggttgc gcatcgaata catagcggcg atcgcacacg tccaccttgg gttcgagatg
     2701 cgccgcctcg aagtgatcgt tgccgatctt gagcatcttc cagaaggcga actgcggatg
     2761 agttcgatgc cgcgccagat tcgccggcgt caagcggaac ggataggcct ggacctggaa
     2821 tgacggccgg ccgagaagcg agtcgcgcgc cagcgaatag atctcgctga tctgttcgtc
     2881 ggtcatggca tagcaaccca tcgacgagca gtcgccgtgg atcatgagca agctgccgtt
     2941 gcggttgttc gccttgtcga agctgttggg gaagccggtg ttgatcgcaa gatagtagtt
     3001 ggaattggga
//
LOCUS       DATLJT010000498         4545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_717743,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000498 DATLJT010000000
VERSION     DATLJT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717743"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VM910_11480"
     CDS             <1..440
                     /locus_tag="VM910_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HVI63188.1"
                     /translation="TMGINPPSNVERVDPKVLHLSGEFAEQNLGTSVAPDGSVTVRII
                     ATQFMFVPRCIAVPHGRPVTLRFAAPDVIHGILITGTNVNTMVVPGFVAQVHTQFTRS
                     GDLLMPCHEYCGLGHSEMWATVQVLPEGAFRPDRDGRVSCAQR"
     gene            427..2043
                     /locus_tag="VM910_11485"
     CDS             427..2043
                     /locus_tag="VM910_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI63189.1"
                     /translation="MRNVERLCLAHFWVAFAAFLVAALLGTWQMWVRSPLGANIGTPG
                     QYFLSVTAHGVSMAYVLSTFFIIGFGYFVAVTALARPLPGKAWAWGAWCVGVIGVVMA
                     VAAILSGQASVLYTFYPPLTGSPWIYVGLVLVVVASWVWCVLMLVAMAAWKRENSGRP
                     VPLAMFATVANAVLWLWTTVGVAVELLFQVIPAAFGLIQTVDVGLSRTLFSWTLHAIV
                     YFWLLPAYIAFYTMVPRAAGGRLYSDTMGRLTFILFLLYSLPVGLHHLLMDPEHGNGM
                     KFLQVLLTGFVTVPTWLTIFTVSASLEIAGRLRGGRGLFGWIAALPWERPMVLAVGLA
                     FVMLGFGGFGGLINMGYGMNAMVHNSSWVTAHFHLIFGGSVVIMYFAIAYEIWPRLTG
                     REYPSLKPLSIQLWLWFIGMMVMTVPWHWLGLQGQWRRVANFNYADPIIAGWGPWVIV
                     SLAGGLVLLASALLFVGNLFALHRSATAAIARPLYALAVHPPQRVPAALNGFGLWNVL
                     VLVLMLVAYGWPIAQFAIEPSPAAIVHRVQ"
     gene            2054..2884
                     /locus_tag="VM910_11490"
     CDS             2054..2884
                     /locus_tag="VM910_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI63190.1"
                     /translation="MSDPRISDPRVLDQPWRIWASVVVIGILLFGVALGVLIIPVVQG
                     RSAGIDMYTAICRALGILPGSPAQPQPATVSPPTPVSKVVWTPEVLQILALGNTERGR
                     AKVLEVCVACHGEQGVSVAPDYPNLNGQSGAAIYKQLHDYRTGSRANPLMTGIANALE
                     EPAIADVAAYYAGQPKRNPNPASLGELPEAIVRLVELGDPNRNIPPCAACHRPGSGGP
                     IETPILAEQGRDYLAQQLRMYASGERRNDVYARMRVIAAKLTPGEIDGLSRYYSAGFR
                     "
     gene            complement(2895..3461)
                     /locus_tag="VM910_11495"
     CDS             complement(2895..3461)
                     /locus_tag="VM910_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63191.1"
                     /translation="MTQAVPINLIRWHYLWYVALALVIMIAAIASHHLWFLNFLHVFS
                     GLLWTGIDLFMGFVLGPILRVVELPARKAVLLRLTPRTLFLMPTLAIVTGTTGWYLAE
                     DMGFTAMSWPQYGWVAAALVLVVLMTIQGIGYLLPTNFRVCLELQKENPDNARIGALT
                     NRYFFAVAFQGVMQIVTIIVMARLATGV"
     gene            complement(3486..3725)
                     /locus_tag="VM910_11500"
                     /pseudo
     CDS             complement(3486..3725)
                     /locus_tag="VM910_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          820 a         1399 c         1398 g          928 t
ORIGIN      
        1 tgaccatggg catcaatccg cccagcaatg tggagcgcgt ggacccgaaa gtattgcatc
       61 tctccggcga gttcgccgag caaaatctcg gcaccagcgt cgcccccgac ggcagcgtca
      121 ccgtccgcat cattgccacc cagttcatgt tcgttccgcg ctgcatcgcc gtgccgcacg
      181 ggcggcccgt gacacttcgt ttcgccgcgc ccgacgtcat tcatgggatc ctgatcaccg
      241 gcaccaacgt gaacacaatg gtggtgccgg gtttcgtggc gcaggtgcac acgcagttca
      301 cgcgcagcgg cgatctgctg atgccgtgcc atgagtactg cggcctcggc cacagcgaga
      361 tgtgggccac ggtccaagtt ctgccggaag gcgcgtttcg gccggatcgg gatgggaggg
      421 tttcctgtgc gcaacgttga gcggctttgc ctcgcgcatt tctgggtggc gttcgcggcg
      481 tttctggtcg ctgcgctgct cggtacgtgg cagatgtggg tgcgcagccc actcggcgcg
      541 aatatcggca cgcccggcca gtatttcctg tcggtgaccg cgcatggcgt gtccatggcg
      601 tatgtgctct ccacgttttt catcataggc ttcggctatt tcgtcgcggt caccgcgctc
      661 gcgcgtccgc tgccgggcaa ggcctgggct tggggcgcgt ggtgcgtggg ggtcatcggc
      721 gtggtgatgg cggtggcggc gattctcagc ggccaggcct ccgtgctcta cacgttctat
      781 ccgccgctga ccgggagccc ctggatctat gtcgggctgg tcctggtggt ggtcgcttcg
      841 tgggtctggt gcgtgctgat gctcgtcgcc atggcggcat ggaagcggga aaattcgggc
      901 cgcccggttc cgctcgcgat gtttgccacg gtagccaacg cggtgctgtg gctatggacc
      961 acggtcggcg tggcggtgga gctgttgttc caggtgatcc cggccgcatt cggcttaatc
     1021 cagacggtcg acgtcggcct ttcccgaacg ctgttttcct ggacgctgca tgccatcgtc
     1081 tatttctggc tgctccccgc ctacatcgcc ttctacacga tggtgccgcg cgccgccggc
     1141 ggccggcttt acagcgacac tatgggtcgc cttaccttca ttctgtttct cctctacagc
     1201 ctgccggtcg gcctgcacca tctgctgatg gatcccgagc acggcaacgg catgaaattc
     1261 ctccaggtcc tgctcaccgg ctttgtaacg gtgccgacct ggctcacgat cttcaccgtc
     1321 tcggcgtccc tggaaatcgc cggccggctg cgcggcgggc gcggattgtt cggatggatc
     1381 gcagcgctgc cctgggagcg cccgatggtg ctcgctgtcg ggctcgcctt cgtcatgctg
     1441 ggcttcggcg gcttcggcgg gttgatcaac atgggctacg gcatgaatgc gatggtgcac
     1501 aacagctcgt gggtcaccgc gcatttccac ctgatcttcg gcggctcagt cgtgatcatg
     1561 tatttcgcga tcgcctacga gatttggccg cgcttgaccg ggcgcgaata tccttcgctc
     1621 aagcctttga gcattcaatt gtggctgtgg ttcatcggca tgatggtaat gaccgtgcca
     1681 tggcattggc tcggattgca gggccagtgg cggcgggtcg ccaatttcaa ctacgccgat
     1741 ccgatcatcg cgggctgggg cccatgggtg atcgtgtcgt tggcaggcgg gctcgttctg
     1801 cttgcatccg cgctgctgtt cgtcggcaat cttttcgcgt tacatcgaag tgcgacggcc
     1861 gcgattgccc ggccgctcta cgcgctcgcc gtccacccgc cgcagcgggt ccccgcggca
     1921 ctcaacggct tcggcctttg gaacgtgctc gtgctggtgt tgatgctggt ggcctacggc
     1981 tggccgattg cccagttcgc aatcgaaccc tcgcctgcgg ccattgtgca ccgtgtgcaa
     2041 taggttcgac gccatgtccg atccacgcat ttctgatccc cgcgttctcg atcaaccctg
     2101 gcggatttgg gcaagcgtcg tcgtgatcgg catcctgctg ttcggggttg cactcggcgt
     2161 tctcatcatt cctgtcgtgc agggtcgcag tgcggggatc gacatgtaca cggccatatg
     2221 ccgtgccctc ggaatcttgc caggctcgcc ggcccagccg cagcctgcga ccgtctcgcc
     2281 tccaactccg gtgtcgaagg tcgtctggac gccggaagtt ttgcagattc tcgcgctcgg
     2341 caataccgaa cgcgggcgtg ccaaggtgct ggaagtgtgc gtcgcctgcc atggcgagca
     2401 gggggtatcg gtcgcgccgg actatcctaa tctcaacggg caatcgggag ccgcgatcta
     2461 taagcagctg cacgactatc gcaccggcag ccgcgccaac ccactgatga ccggaatcgc
     2521 gaacgctctg gaagagccgg ctattgccga cgtggcggcc tattacgccg ggcagccgaa
     2581 acgcaatccg aatcccgcaa gcctcggcga gttgccggag gccatcgtgc gcctagtcga
     2641 actcggcgat cccaatcgca atatcccgcc gtgcgcggcc tgccaccggc cgggatcggg
     2701 cggccccatc gaaacgccga tcctggccga gcaaggccgt gattacctgg cgcaacagct
     2761 gaggatgtac gcctcgggtg agcgccgcaa tgacgtctat gcgcggatgc gagtcatcgc
     2821 ggcaaaactg acacccggcg agatcgacgg gctgtcccgc tattacagcg ccggctttcg
     2881 ctgaaagcga cacctcagac tcccgttgcc aatcgcgcca tgacgatgat cgtcacgatt
     2941 tgcatcacgc cctggaacgc cacggcgaag aaatagcgat tggtcagcgc gccgatcctc
     3001 gcattgtccg gattttcttt ctgcagctcc aaacagacgc ggaaattcgt gggcagcaaa
     3061 taaccaattc cctgaatcgt catgaggacg accagcacga gcgcggctgc cacccatcca
     3121 tattgtggcc agctcatcgc cgtgaacccc atatcttcag cgaggtacca accggttgta
     3181 ccggtgacga tggcgagtgt cggcatcaga aacaatgtac gtggggtcag gcgcaagagc
     3241 acggcttttc gcgccggaag ttcaacgacg cgcaagatcg gaccgagcac gaagcccatg
     3301 aacaggtcga tcccagtcca caacaagccg gagaacacat ggagaaaatt cagaaaccag
     3361 agatggtggc ttgcgattgc ggcgatcatg atgacgagcg ccagagcaac gtaccataaa
     3421 taatgccaac gaatgaggtt gattggcacg gcctgagtca tcacccgctt cagagtgccc
     3481 gcacgctagt ggcggatggc aagcttcgca acacaagcgt cgggatcgct aggatcaggc
     3541 gaaatggaga acccgagctc tctgcacatc gccagcatcg tggtgttttc gcttagcacc
     3601 tggccctcga tggtcttgag gccctcgaca cgggcatagt cgatgatcat ctgcatgagc
     3661 aggtagccga gaccgcgccc tttgagcttg gaccgcacga gaatggcgta ttcgccgcgc
     3721 tcgtagaagc ggaccgaccg cggtcagcag cgcgagtacc gccgtgagcg cgaaagttca
     3781 ggacgcagca tgacgcaccc cgaactatag gccacatgag ccagcccgct ctttgatcta
     3841 tcgcaaccgg gccccacagt ggctgccccg tagcagcgac ttttgaaccc gtcgacaagt
     3901 tgacctaagt caaaaaagca ccggctcacc cggcctatat ttttcctaat gccaagtcat
     3961 gggctgcaac aggaggaggg ataaatgccg accctgtccg ttgaacaaaa aagcttgacc
     4021 aaagccgttt cgcaatggtg gcgacaatgg accagaagag gttccgcaag attcgaactg
     4081 aaatgctgcg gcgaggagga agtggagcgc atggctaaag atatcggcgt gttcccttcc
     4141 gaactgcgca agcttgcgac tcttggtccc gactcagccg atcttctgct gcgccggatg
     4201 gcggcgctcg acctcgaccg aaaggaggtg tcgcaaaccg aacctcgaac gttccaagac
     4261 cttcagcgag tttgcacaat gtgtgaccat catcgccgct gccggcggga ccttgcgcgc
     4321 gattcggctg atccggcatg gcaggattac tgcccgaacg ccgcgacgct catggcgcta
     4381 aacgcgctcc cttggcgtgc gcggagtgag tggtaactag tggccagctg cgcgcaaagg
     4441 tcgctttgca aaagttgctt gcgttatgga tggcggtatc cccaaggagg cagaaatgaa
     4501 gtcagccaag ctagtctctg gagtcttgct gggcctcaca ttgct
//
LOCUS       DATLJT010000499         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_488939,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000499 DATLJT010000000
VERSION     DATLJT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488939"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..232
                     /locus_tag="VM910_11505"
     CDS             41..232
                     /locus_tag="VM910_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63192.1"
                     /translation="MPPFIAFAGVLGGLAVIRWAYKTAVRINQELEEQRLARVAESAR
                     SSEIKTLRRDPVTGAYRPG"
     gene            505..1440
                     /locus_tag="VM910_11510"
     CDS             505..1440
                     /locus_tag="VM910_11510"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HVI63193.1"
                     /translation="MDMLPAHMRPERSFQGLILTLQRYWADCGCVILQPYDMEVGAGT
                     FHPATTLRALGPKAWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQVILKPSPPDIQEL
                     YLKSLAAIGIDSSLHDIRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVAGVE
                     CAPVSGELTYGLERLAMYVQGVDRVYDLNFNGREGAEKVTYGDVFLQAEQEYSRHNFE
                     LADTDMLFEQFRMAEEACKKYLAAGWRDVGNRKEHLMALPAYDQCIKASHVFNLLDAR
                     GVISVTERQSYILRVRELAKACGEAWVHTEAGRAA"
     gene            1530..>2872
                     /gene="glyS"
                     /locus_tag="VM910_11515"
     CDS             1530..>2872
                     /gene="glyS"
                     /locus_tag="VM910_11515"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVI63194.1"
                     /translation="MPDLLLELFSEEIPARMQAKAADDLRRMVTDRLVAEGLVYEGAK
                     AFATPRRLALTVHGIPIRQPDLKEERRGPRVGGADAAIQGFLKATGLASIGQAKIERD
                     PKKGDFYVALIEKKGRATLDVLADILPVIIRTFPWPKSMRWGARSARSGALTWVRPLH
                     AITATFGLETEETDVVKFAVDGIEAGQTTFGHRFMAPGAINVRRFEDYDAKLLAANVV
                     LDPDRRKDSILTDAKQLAFAQGFDLVEDLPLLDEVAGLVEWPVTLMGSFDPEFLSIPG
                     EVIRATIRNNQKCFVVADPKTGKLANKFVLAANIEAADGGKAIVAGNERVIRARLADA
                     KFFYETDLKTKLEDRLPKFDAIVFHEQLGTQAERIARIERLAGELAPLVGADVEKARR
                     AARLAKADLLTEVVGEFPELQGLMGKYYALAQGEDASVAAACEEHYKPQGPADRVP"
BASE COUNT          533 a          870 c          928 g          541 t
ORIGIN      
        1 caatgcttcc agggggcatg atatggacaa ggatcgaccg atgccgccgt tcattgcatt
       61 tgcgggcgtg ttggggggac tggctgtcat ccgctgggcc tacaagacgg ccgtgcggat
      121 caatcaggaa ctggaagagc agcggcttgc gcgcgtcgcc gaatccgccc gatcgagcga
      181 gatcaagacg cttcgccgcg atcccgtgac cggcgcctat cgtccgggct gagaggaaaa
      241 tcggttttga cgctgtttga aaacagccgt ggagcggctg cctattcgcc gcctctcccc
      301 gatgcgaacg tcaaatccaa agctccactg gcatcattaa tttgcgggta gtctttagct
      361 tccaacattc gcaaagtgcc tcccggatgg cctgcgaacg ttggaatcgg accgctggct
      421 gccgccgtcg ccttgattcc cgtcctcgcc gccgatacgg tcccgcgcat tttcgccagc
      481 tcacccgcga gacctgatcc gacgatggac atgctgcctg cccatatgcg cccggaacgt
      541 tcgttccagg gattgatcct gacgctgcag cgctattggg cggactgcgg ctgtgtgatc
      601 ctgcagccct acgacatgga ggtcggggcc ggcaccttcc atcccgcgac cacgctgcgc
      661 gcgctcggtc ccaaagcctg gaatgcagcc tatgttcagc cgtcgcgccg tccgaaggac
      721 ggccgctatg gcgagaatcc caaccgcctc cagcattatt accagttcca ggtcatcctg
      781 aagccgtcgc cgccggacat tcaggagctt tatctgaaat cgctcgcagc gatcggcatt
      841 gattcctcgc tgcacgacat ccgcttcgtg gaagacgatt gggagagccc gacgctcggc
      901 gcctgggggt tgggctggga atgctggtgc gacggcatgg aggtctcgca gttcacctat
      961 ttccagcagg tcgccggcgt cgaatgcgcg ccggtttcag gggagctgac ttacgggctc
     1021 gagcggcttg cgatgtatgt gcaaggcgtt gaccgcgtct acgacctcaa tttcaacggc
     1081 cgcgagggcg ccgagaaggt gacttatggc gacgtgttcc tgcaggccga gcaggaatat
     1141 tcgcggcaca atttcgagct tgccgacacc gacatgttgt tcgagcagtt tcggatggcc
     1201 gaagaagcct gcaagaaata tctcgcggcc ggctggcggg acgttggtaa ccgcaaggag
     1261 catctgatgg cgttgccggc ctatgatcaa tgcatcaagg cgagccatgt gttcaacctg
     1321 ctcgatgccc gcggcgtgat ctcggtgacg gagcggcaga gctacatctt gcgcgtccgc
     1381 gagctcgcga aagcctgcgg cgaggcctgg gtgcacacgg aagcggggag ggcggcgtga
     1441 gagttgtcgc gcggcgactc ggattgcctc cggcaggcga ccctccccct caaggggagg
     1501 gtggttcttt gcgtggggaa tggtgaaaaa tgccggacct gctcctggaa ctgttctccg
     1561 aagagatacc tgcgcgcatg caggcgaagg cggccgacga tttgcgccgc atggtcaccg
     1621 acagactggt ggcggaaggt ctggtctatg aaggtgccaa ggcttttgcg acgccgcgtc
     1681 ggctggcgct taccgttcac ggaatcccca tacgccagcc ggatctgaag gaagagcgca
     1741 ggggtccgcg cgtcggcggc gcggatgcgg cgatccaggg cttcctcaag gcgacggggc
     1801 ttgcatcaat cgggcaggcg aagatcgagc gcgatcccaa gaaaggcgac ttctacgtcg
     1861 cgctgatcga gaagaagggc cgcgccacgc tcgacgtgct cgccgacata ttgcccgtga
     1921 tcatccgcac cttcccctgg ccgaaatcga tgcgctgggg cgcacgctcg gccagatcag
     1981 gcgcgcttac ctgggtgcgg ccgctgcacg cgatcaccgc gaccttcggg ctcgagaccg
     2041 aagagaccga tgtggtgaaa ttcgcggtcg atggcatcga ggctggccag accactttcg
     2101 gtcatcgctt catggcaccc ggcgccatca acgtgcgccg cttcgaggat tatgacgcaa
     2161 agctcctggc tgcgaacgtc gtgctcgatc cggaccgtcg caaggactcc attctgaccg
     2221 acgcgaagca gcttgcgttc gcgcaagggt tcgatctcgt cgaagacctt ccgctgctcg
     2281 atgaagtcgc gggcctcgtc gaatggccgg tcaccttgat ggggtcgttc gatccggaat
     2341 ttctgtcgat cccgggcgag gtgatccgcg ccaccatccg caacaatcag aagtgctttg
     2401 tggtcgctga tcccaagacg gggaaactcg cgaacaagtt cgttctcgcc gcgaatattg
     2461 aagcagccga cggcggcaag gcgattgtcg ccggcaatga gcgcgtgatc cgcgcacggc
     2521 tcgccgatgc gaagtttttc tatgagaccg acctgaagac gaagctggaa gatcgtttgc
     2581 cgaaattcga tgccatcgtc ttccacgagc agctcggcac gcaggccgag cgcatcgcgc
     2641 gcatcgagcg gctcgccggc gagcttgcgc cgctcgtcgg cgcggatgtt gaaaaggcca
     2701 ggcgagccgc gcgtctcgcc aaagctgatc tgcttacgga ggtcgtgggc gaattcccgg
     2761 aattgcaggg cctgatgggg aaatactacg cgctcgccca gggcgaggat gcctccgtcg
     2821 ccgccgcctg cgaggagcat tacaagccgc aagggcctgc cgatcgcgtg cc
//
LOCUS       DATLJT010000500        17728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1866976,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000500 DATLJT010000000
VERSION     DATLJT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866976"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VM910_11520"
     CDS             complement(<1..1866)
                     /locus_tag="VM910_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVI63195.1"
                     /translation="MNIEMPRRQLDLASAIAEGDIRCLLMVLVHMTGDERWLEPPYKP
                     RRDVRLIPDPEAGLAKDIQDEIRAAVVKLFAQGNPKPVVTDPGNDLMLKMMRACLGEN
                     VAGEYAPLMREEMGFVPRDARWRNRPSDAALSRQHALIVGAGVCAIALGVALGRLGIP
                     YTIVEKNDELGGTWYVNRYPGCGVDTPNHSYSYSFGARNAWTRYFCPREELLDYLKKV
                     AAEYGIRDHLRLGTRLTSSRWDDRKLCWISTLKTKDGEETFESTVLVSAIGQLNDPSP
                     AHFKGEEDFKGLILHSALWNDDIDVTGKRVAVIGSGATAMQLVPTIADRVASVTVYQR
                     TAQWARPVQGYSDPITEGARWLLAHLPFYVQWYRFNMFWRYGDGLLPFLRKDPAWPHP
                     ERAVNKGNDRHRQELTDFILSELKDRPDLIDKCVPTYPPYGKRILLDNGWFRTLTKPN
                     VELVTDTIDHFARDGIVTADGKLRPFDIIVIATGFKVTEMAARLNISGRDGRNLRDKW
                     ANDNPTAHLGLTVPGFPNFFCMLGPNSGPAHGGSVIFQSECQSRYISACLVDMIERGV
                     AAIDVRQDVLDDYVRKVDAEHEAMIWTHPGMTTYYRNKNGRVFSAMPWRFVDYWAM"
     gene            complement(1863..1994)
                     /locus_tag="VM910_11525"
     CDS             complement(1863..1994)
                     /locus_tag="VM910_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63196.1"
                     /translation="MSADDSLVTGANLPVDADFIAGSSRDAYGGLREAQVQHEGMRS"
     gene            complement(1984..2640)
                     /locus_tag="VM910_11530"
     CDS             complement(1984..2640)
                     /locus_tag="VM910_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI63197.1"
                     /translation="MTQGGAASAPVKVTKLNRVERNAWTKQKIFDAATKVVGKYGYAE
                     ASVARITEEAGVAQGTFYNHFENRQELLEQLLPKIGIDMVQFIRARTNTADAARQEIE
                     RFSAFFDFIREVPEFLRILNEAEFFAPRGYQQHLDNISTAYVRLLRRARLGGAIEDYT
                     DDEFEAIVHMLMGARGYLSRRYSYSSGGVTAVPDHVISAYRKLMTRGLFNSSGGDDER
                     "
     gene            complement(2637..4133)
                     /locus_tag="VM910_11535"
     CDS             complement(2637..4133)
                     /locus_tag="VM910_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI63198.1"
                     /translation="MINLSSFIAFHARRTPDRCALKYRGEDITYGAFDARIGAVAGWL
                     VAQGIGPDDVVAVLMKNSAAFLELAFAVSHIGAVFLPINYRLSRDEVGYIVGNAEARI
                     LIADAELGEIAGGSAPVVLIDEAAQTSLTLVASDATLAPLVPRKPQDLMRLMYTSGTT
                     DRPKGVMLTYENIYWKSADQTIVLGLNADTRLLVVGPLYHVGALDLPGIAVLWHGGLL
                     SVQRNFEPEQALAAIQAEKLTGAWFAPVMTTAILTCPNRDSYDVSSFKWAIGGGEKTP
                     EARIRSFSEFFTNACYIDAYGLTESCGGDTFMEAGREIEKIGSTGRAIAHVEIEIRDE
                     AGRCLPAGATGEICLRGPKITKGYWMDPAKTASAFFGDWFRTGDVGHLDEDGFLYLTD
                     RKKDMIISGGENIASSEVERAIYEMPQVREVAVVGLPDERWGERPVAVVVLADNAALE
                     LSDLAAHCRTRLAGFKVPKQLVIRDALPRNPSGKVLKRVLRSELEAKA"
     gene            4298..5557
                     /locus_tag="VM910_11540"
     CDS             4298..5557
                     /locus_tag="VM910_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63199.1"
                     /translation="MTRTRTPGISRRSSLMIMGGGALALSAPWVARAEAKTVKIGMPT
                     ILSGRVAQLGTSSRNAVMLEVDKINAAGGLAGRQIEMVIRDSKGQPQEAARVARDLVN
                     TDGCELLIDAEASSGAFAVHEVARDLGVLCIHTNSETSSLTADPKQHIPNAFRTARQG
                     VHDSIVGGSYAATIAKAKGLKKWATCSPDYAYGRDTTAEYSFYLKRFSPDVEIISESW
                     PKLFQPDYTEVVTKILQAKPQALYTCLWGGDLTSYIDQANIYAMFGQMEVFAVNMADY
                     TALTVVKNLPKGIHSGNRYIKTFPATPDNAAWGDAYKAKYNEYPTNWSWENATAMMLL
                     AEASKKANSADGKKIAEALRGLTIKCPFGADGTVTMRAEDQTLIGYAIGWGTTIPQEP
                     YVPNVQAGDWKTILALEAEWKKDKGYA"
     gene            5653..6555
                     /locus_tag="VM910_11545"
     CDS             5653..6555
                     /locus_tag="VM910_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63200.1"
                     /translation="MDLDALAGCLASSACLVTQTTSGLIIGMLLFLVAVGLTLIFGVL
                     KVVNFTHGAFYMFGAYFAMTSYQATGSFVLAIVCGAAGTAILGLIFERVFMSRVYGAN
                     VLMQLLVCYAFVLIFDDVVRLVWGPEFKSMGMPAAFQVMPLFIAGGVVPPYYLLLIGV
                     ALAAAIVLGVGLARTRLGKVIRAAADNPGMVSALGINTGALYGAVFALGCMLAGLAGA
                     LAAPVRSLTPGMGFSVLIESFIVTVIGGMGSISGALIGALLLGLIRSFGSQGFPLFTD
                     GLMYLFMVIVLVARPTGLFGKEVA"
     gene            6552..7580
                     /locus_tag="VM910_11550"
     CDS             6552..7580
                     /locus_tag="VM910_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63201.1"
                     /translation="MTELQAERATKTLTTPRDDAASKGWRYLDVLIALAAFAVLASLP
                     FLSSSKALLDFVIRCSAYGLFATSLNLLVGYTGLTSFGHGMFFGLGAYSFGLIMQRTG
                     ASIPLAFVATLAITAIVATVIGAICVRLKEIYFAFVTLAFQMLIYSTILSWPSFTGGD
                     QGLRGGIPRPVFIGIDLSNHVQLYLFSCALLVLGLLAMRQIVQSPFGYTLRMIRDNAK
                     RAAFIGIHVWRAKLAIFVMAALFAAIGGMVMALFVSGAYPEFAYWTISGEGIFINMLG
                     GVTTFLGPMVGTVLLLILNDTVTRFTDYHGIVLGIVILFFALGLRKGLMDFVVDWAKQ
                     RREKGGSA"
     gene            7583..8338
                     /locus_tag="VM910_11555"
     CDS             7583..8338
                     /locus_tag="VM910_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63202.1"
                     /translation="MLEIRGLSKSFGGVRATDNVTLDFADGSLTAVIGPNGAGKSTFF
                     NLITGAFRPDSGKILLDGYDLAGSAPAEIVRHGIGRAFQVASVFPSLTVEETMLAAVG
                     ADQRRAGVLHRRFPLPEMRDRAEHAMELLGLAGKRNRVASTLSHGDQKLLDIALALVL
                     DPKVLLLDEPTAGMGPEERWRMIDKVRELWDTQKITVVFIEHDMDIVFKIAPEIVVLC
                     YGRILASGTPAAIRSNAAVIEAYLGTEHAGGSA"
     gene            8335..9051
                     /locus_tag="VM910_11560"
     CDS             8335..9051
                     /locus_tag="VM910_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63203.1"
                     /translation="MSGQAIMELADLDVYYGTSQILFGVGLSVRQGETMALLGRNGAG
                     KSTTMKAIMGLAPPRRGTVSLRGEAIAGLKPHRIARAGLGFVPEDRQIFPDHTVEDNL
                     LVGRKKGPRGEDDWPLARIYQVFPLLEPLRHRIAGRLSGGEQQMLAIARTLMGNPTLL
                     LLDEPSEGLAPIIVQRIGELLRQLRAAGATVLIAEQNLHFCLGVASHATVIDKGQIVH
                     SSTIEELKANDDIRRRYLAL"
     gene            complement(9175..9675)
                     /locus_tag="VM910_11565"
     CDS             complement(9175..9675)
                     /locus_tag="VM910_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI63204.1"
                     /translation="MRQVLEKNGVADLDRIDLKILAELMGDARASQLELAEKVGLSPT
                     ACARRIRHLEEGGIIRGYRADLEPTALGLVTTVVVTITLEKQSEDYLAAFEAAIARCP
                     DIVSCHLMSGSDDYHLQVLARDIADFERIHKQHLSRMPGVARIHSSFAMREVVRRAIP
                     ENALRG"
     gene            9789..10904
                     /gene="ald"
                     /locus_tag="VM910_11570"
     CDS             9789..10904
                     /gene="ald"
                     /locus_tag="VM910_11570"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HVI63205.1"
                     /translation="MRIGVPKEIKVEEYRVGLTPASVREYVARGHEVVVEHGAGQGIG
                     AGDDVYRAAGAVVAETAEEVFATAEMIVKVKEPQATEWVRLRENQILFTYLHLAPDSA
                     QTKGLLASGCTAIAYETVTDAHGGLPLLAPMSEVAGRLAIEAAGAALRKTTGGMGKLI
                     GGVPGVLPARISVIGGGVVGTHAARMAVGLGGDVTILDRSLPRLRALDELFGGRVRTR
                     YSTLEAIDEEVAGADVVIGAVLVPGAAAPKLVTRAHLATMKPRAVLVDVAIDQGGCFE
                     TSRPTTHQAPTYLVDDVVHYCVANMPGAVPVTSSYALNHATLPFGLALAAKGLRALVE
                     DPHLRAGLNIHRGQITNRAVAESQNAAAVAPEAALAA"
     gene            complement(10922..11743)
                     /locus_tag="VM910_11575"
     CDS             complement(10922..11743)
                     /locus_tag="VM910_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVI63206.1"
                     /translation="MSRQSIDTGTDELLCEIADKVAIITLNRPEARNALSDHLTPALR
                     RMIKQCGEDDRVGSILITGSGNAFCAGGDVKGMGDSAPKAETSFEDRVANLRTKQRTL
                     TGALVAVRKPTIAALPGPAAGAGMAIALACDIRIAAVSAFMSTGYARIGLTGDYGIAW
                     LLTRLAGTARARELMFLSERLDARRCEAIGLINRVVPDNELREAAFALARQLAEGPTF
                     ALGRIKDNLDQALTSSLLDSMDQEAENLVRAARTTDHKEAVRAFVEKRKPSFVGQ"
     gene            12060..13259
                     /locus_tag="VM910_11580"
     CDS             12060..13259
                     /locus_tag="VM910_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63207.1"
                     /translation="MMRALWVACALLLALETPSLAADTPGVTPTEIKVGGIFPFSGPA
                     SSIGLVGRGVLAYVQSINDRGGINGRKINYIAYDDAYSPPKAVEHARKLVEADEVAFI
                     FSQLGTPGNTATAKYLKSKGVPTIAIVSGSNKFTDVANFPLTTTSLVSFDTEGRIYAK
                     FLNRTLPDAKYAILYQNDDLGKDYVNAFRELLKSDFDRKVVAAAYEISDPTIESQIVN
                     LKSSGAGALLVAGTPKFAAQAIRKAHELGWKPMILLNYPSSSVGATLKPAGLDVSTGV
                     IVGTVTMDPADSQWDQNEGMKNFRTFVDKYMPGVDIADTNYLFGYTQGMLLEQLIKQC
                     GDDLSRENIIKQSRSFHDLVLPTVLPGIKINTSNKINMNYTQMRLQRWTGTHWDLFNE
                     VLDASSE"
     gene            13298..14860
                     /locus_tag="VM910_11585"
     CDS             13298..14860
                     /locus_tag="VM910_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI63208.1"
                     /translation="MHQTEPTIEEITASLPRRLHEVAQRYVVDAPDRAAMIEDGAKWT
                     YRDLDRYVRQTAERLTAFGVRAGDRIMLVSENCIALAALLLAASRIDAWAIVSNPRLS
                     ARELDQIRDHSGARRVFFTCAVSKEAAAHAARYGAEILSFGPLSDIGVGPLNEAAAAE
                     PVEFDSARQVAVLIYTSGTTGKPKGVMLSHRNLLVSAGTTALFRRMTEQDKVYVVLPI
                     SHIVGISLLIMTLMVGGSVRLVSKYDPAALAKAMAEEGITILNGVPATYQRLLEYKTV
                     SGMKRLERGALRYMGVAGAPLDLELKARAENEIGLPLSNAYGITECSPGISGVRLDAP
                     RSDEAVGTLVPGVEARVRTLDGIPVGNGEIGELHVRGPNVMLGYYREPALTANVIDSE
                     GWFNTGDLARFEGDVLYIVGRTKEMIIRSGFNVYPAEIEAVLNSHKDVVQSAVVGRKA
                     EGNEEVIAFVQLISGSRIGATELMNYVAPQLTAYKRPSQIIVLDALPATSTGKILKHS
                     LAESLRVPAAVS"
     gene            complement(14918..15433)
                     /locus_tag="VM910_11590"
     CDS             complement(14918..15433)
                     /locus_tag="VM910_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI63209.1"
                     /translation="MKWDALEEEPCSVARTIGVIGDRWTLLILRECFLRTRRFEGFQS
                     ALGITRHLLAERLKKLVRLGILRRIPYQESPKRHEYILTQKGLDLYPIMMAIVHWGDT
                     HMVDERGRPLLHEHKKCGKLFDPVMVCSECGEPLSAKEVTTRPGPGARPDPSKLGAAG
                     AEKPKSRTHAA"
     gene            15667..16305
                     /locus_tag="VM910_11595"
     CDS             15667..16305
                     /locus_tag="VM910_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVI63210.1"
                     /translation="MAKPLRVEFHFDFGSPNAYLAELVLPEIERRTGVKFDYVPVLLG
                     GVYKATGNMSPFDSLRGIKNKPEYNALETQRFLRRHNITKFQSNPFFPVNTLTLMRGA
                     VAAQFEGVFESYFRAAYHHMWVEPKKMDDPAVFREAFLSSGIDIDRLIARAQQEDVKK
                     KLIENTSDAVARGSFGSPTFFVGNEIFFGKDSLRDVEEEIVAQLNVARRKTA"
     gene            16377..>17728
                     /locus_tag="VM910_11600"
     CDS             16377..>17728
                     /locus_tag="VM910_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI63211.1"
                     /translation="MTSEQAERGAAAASILASLPNRIHQVLDHHVAATPDSVALVEDG
                     ESLTYRQFDRAVSATTDALRELGVRPGDRVVIVSENCIPLACLLFAASRLDAWAIVAN
                     PRLSPRELNQITEHSGARRVFFTADVSEEAAAHAMRLGATIADIGPLSGIGFSALNVD
                     TIAEPVEADGAKQVAVLIYTSGTTGTPKGVMLTHRNLLFSAMTTALHRDMTAADNQYC
                     VLPVSHIVGISLLIMTLMVGARVRMVVKYSPAALVKAMAEEGITLLNGVPATYQRLLE
                     YKATAGLPKLERGALRLIGVAGAPLDLDLKARVEKELGLPLSNGFGITECSPGISGVR
                     SDAPRDDNAVGRLLPGLEARIVRRDGTIVANGDIGELHVRGPNVMRGYYRAPDLTAKA
                     IDKDGWFNTGDLARFEEDVLFIVGRTKEMIIRSGFNVYPAEIEAVISTHPAVVQSAVV
                     G"
BASE COUNT         3217 a         5495 c         5643 g         3373 t
ORIGIN      
        1 catcgcccaa tagtccacga accgccatgg catcgcggaa aacacgcggc cgttcttgtt
       61 gcggtaatag gtcgtcatgc ccggatgggt ccagatcatc gcctcatgct cggcatcgac
      121 cttgcgcacg taatcgtcga gtacgtcctg gcgcacgtcg atcgcggcga cgccgcgctc
      181 gatcatgtcg accaggcagg ccgagatata gcggctctgg cattccgact ggaagatcac
      241 gctgccgcca tgcgccggcc ccgaattggg accgagcatg caaaagaaat tgggaaaacc
      301 gggcaccgtg agcccaagat gcgcggtcgg gttgtcgttt gcccatttgt cgcggaggtt
      361 ccggccgtcg cgaccgctga tgttgaggcg cgcggccatc tcggtgacct tgaagccggt
      421 cgcgatcacg atgatgtcga aggggcgcag cttgccatcg gccgtgacga tgccgtcgcg
      481 tgcgaaatga tcaatcgtgt cggtgaccag ctcgacattc ggcttcgtca gcgtcctgaa
      541 ccagccgttg tcgagcagga tgcgcttgcc atagggcgga taggtcggca cgcatttgtc
      601 gatcaggtcg gggcggtcct tcaattccga cagaatgaag tcggtcagct cctggcggtg
      661 ccgatcgttg cccttgttga ccgcgcgctc gggatgaggc caggccgggt ccttgcgcag
      721 gaagggcaac agcccgtcgc cgtagcgcca gaacatgttg aaacggtacc actgcacgta
      781 gaacggcaga tgcgcgagca gccatcgcgc accctcggtg atcggatcgg aatagccttg
      841 caccggtcgc gcccattgcg cggtgcgctg atagacggtg accgaggcca cacgatcggc
      901 gatcgtcggc accagttgca tcgcggttgc gccgctgccg atcaccgcga cgcgcttgcc
      961 ggtgacgtcg atgtcgtcgt tccagagcgc ggagtgcagg atcaggccct tgaagtcctc
     1021 ctcgcccttg aaatgcgcgg gcgagggatc gttgagctgg ccgatggcgc tgacgagaac
     1081 cgtcgattcg aatgtctcct cgccgtcttt cgtcttcagt gtcgagatcc agcacagctt
     1141 gcgatcgtcc cagcgcgacg acgttagtcg cgtgccgagc cgcaaatgat cgcgaatgcc
     1201 gtattcggcc gcgactttct tcaaatagtc gagcaattcc tcgcgcgggc agaagtacct
     1261 cgtccaggcg ttgcgcgcgc cgaacgagta ggaataggag tggttcggcg tgtcgacgcc
     1321 gcagcctgga tagcggttga cgtaccaggt gccgccgagt tcgtcgttct tctcgacgat
     1381 ggtgtagggg atgccgagtc gtcccagcgc cacgccgagc gcgatcgcgc agacgccggc
     1441 gccgacgatc agcgcgtgct gccgcgaaag cgctgcatcg gacggccgat tccgccagcg
     1501 cgcatcgcgc ggcacgaagc ccatctcttc gcgcatcagc ggggcgtatt cgcccgctac
     1561 gttttcgccg agacaggcgc gcatcatctt cagcatcaga tcgttgccgg gatcggtgac
     1621 gacaggtttc ggattgccct gcgcgaacag cttgacgacc gcagcgcgga tctcgtcctg
     1681 gatatccttt gcaagcccgg cttccggatc ggggatcagg cggacgtcgc gccggggctt
     1741 gtacggcggc tccagccacc gctcgtcgcc ggtcatgtgc accagcacca tcagaaggca
     1801 gcggatgtcg ccctcggcga tggcggaggc aaggtcaagc tgtcggcgcg gcatctcgat
     1861 gttcatgatc tcatgccctc atgttgaact tgtgcttcgc gtaatccgcc ataggcgtcc
     1921 cggctcgagc ctgcgataaa gtcggcgtcg accggaagat tggcgcccgt aacgaggctg
     1981 tcgtcagcgc tcatcgtcgc ctccggacga gttgaagagg ccgcgcgtca tcagcttccg
     2041 ataggccgag atgacgtgat cggggaccgc cgtcacgccg ccgctggaat aggaatagcg
     2101 gcggctgaga tagccacgcg cgcccatcag catgtggacg atcgcctcga actcgtcgtc
     2161 ggtgtaatcc tcgatcgcgc cgccaagccg cgcgcggcgc agaaggcgca cgtaggcggt
     2221 ggaaatattg tcgagatgct gctggtagcc gcgaggtgca aagaactcgg cttcgttgag
     2281 gatgcgcagg aactccggga cctcgcggat gaaatcgaag aacgcggaaa agcgctcgat
     2341 ctcctgccgc gccgcatccg ctgtgttcgt gcgcgcgcgg atgaactgca ccatgtcgat
     2401 gccgatcttg ggcagcagtt gctccagaag ttcctggcga ttttcgaaat ggttgtagaa
     2461 cgtgccctgc gcgacgccgg cttcttccgt aatgcgggca acggaggctt ccgcatagcc
     2521 gtatttgccg accaccttgg tcgcggcatc gaaaatcttc tgcttggtcc aggcgttgcg
     2581 ttcgacgcgg ttgagcttcg tcactttcac cggcgcgctg gcggcacctc cttgcgtcat
     2641 gccttcgcct ccagttcgct gcgcagcacg cgcttgagca ccttgccgga cggattgcgg
     2701 gggagagcgt cgcggatgac gagctgcttc ggcactttga aaccggcaag gcgcgtgcgg
     2761 caatgcgcgg cgaggtcgga gagctcgagc gcagcattgt cagcgagcac gacgaccgcg
     2821 accggccgct cgccccagcg ctcgtcgggc aggccgacca ccgccacctc gcgcacctgc
     2881 ggcatttcgt agatcgcccg ctcgacctcg gacgaggcga tgttctcgcc gccggaaatg
     2941 atcatgtcct tcttgcggtc ggtgaggtag agaaagccgt cctcgtcgag atggccgacg
     3001 tcgccggtgc gaaaccaatc gccgaaaaac gccgacgcgg tctttgcggg atccatccag
     3061 tagcctttcg tgatcttcgg gccgcgcaga cagatttcgc cggtcgcgcc ggcgggaagg
     3121 catcggcccg cctcatcgcg gatttcgatc tcgacatggg cgatggcgcg gccggtcgat
     3181 ccgatcttct cgatctcgcg gccggcttcc atgaatgtgt cgccgccgca actctcggtc
     3241 aggccatagg cgtcgatgta gcacgcgttg gtgaaaaact cggagaaaga ccggatgcgc
     3301 gcttcgggcg ttttctcgcc gccgccgatc gcccatttga agctggagac gtcgtagctg
     3361 tcgcggttgg ggcaggtgag gatcgcggtg gtcatgacag gcgcgaacca tgcgccagtg
     3421 agtttttccg cttggatcgc agccagcgcc tgctcgggct cgaaattgcg ctgaaccgac
     3481 agcaggccgc catgccagag cacggcaatg ccgggcaaat cgagcgcgcc gacatggtag
     3541 agcggaccga cgaccagaag ccgcgtgtcc gcgttgagcc cgaggacgat cgtctgatcc
     3601 gcggatttcc agtagatgtt ctcgtaggtc agcatcacgc ccttgggacg atcggtcgtg
     3661 ccggacgtgt acatcagccg catcaaatcc tgcggcttgc ggggaaccag cggcgccagc
     3721 gtcgcatctg atgccaccag cgtgaggctc gtctgtgcgg cttcatcgat cagaacgacc
     3781 ggtgcgctgc cgcctgcaat ctcgccaagt tcagcgtcgg cgatcaggat gcgcgcctcg
     3841 gcgttaccga cgatgtagcc gacctcgtcg cgcgagagcc gatagttgat cggcaaaaac
     3901 accgcgccga tgtgactgac tgcaaaagca agttcgagaa aggcggcgct gttcttcatt
     3961 agcaccgcga ccacgtcgtc ggggccgatc ccttgcgcga cgagccagcc tgcgacagcg
     4021 ccaatgcgcg catcgaaggc gccataagtg atgtcttcgc cccggtactt cagggcgcaa
     4081 cggtccgggg tgcgcctggc gtgaaaggcg atgaagctgg acaggttgat cattcgcggg
     4141 gcggcgcctc tctggcctat tcctcccaag atgaattatg agtcgtaatt catacttcgc
     4201 ttgacgtcac cccccttact ctgaaattct tcagcatggg agaacgcgcg atttccaata
     4261 aggaaacggc gaaacgcaca gacgacaggg agagaacatg acgagaaccc gcacgccggg
     4321 catcagccgc cggtcgagtc tcatgatcat gggtggcggc gcgctcgcgc tgtcagcgcc
     4381 atgggtcgcg cgcgccgaag ccaagaccgt caagatcggc atgccgacca ttttgtcggg
     4441 ccgcgtcgcg cagctcggca cgtcgtcgcg caacgcggtc atgctggaag tcgacaagat
     4501 caacgcggcc ggcggcttgg caggacgcca gatcgagatg gtgatccgcg attccaaggg
     4561 gcagccgcag gaagcagcac gggtggcgcg cgatctcgtc aataccgacg gctgcgagct
     4621 cctgatcgat gccgaggcgt cctccggcgc cttcgccgtg cacgaagtcg cgcgcgatct
     4681 cggagtgctt tgcattcaca ccaattcgga aacctcttcg ctgaccgccg atcccaagca
     4741 gcacattccg aatgccttcc gcaccgcgcg ccagggcgtg catgactcca tcgtcggcgg
     4801 cagctatgcg gcaacgatcg cgaaggccaa gggcctgaag aaatgggcga cctgttcgcc
     4861 ggactatgcc tatggccgcg acacgacagc cgaatactcg ttttacctga agcgcttttc
     4921 gcctgacgtc gagatcatca gcgaatcctg gccgaaactg ttccagcccg actacaccga
     4981 agtggtgacg aagatcctgc aggccaagcc gcaggcgctc tacacctgcc tgtggggcgg
     5041 cgatctcacc tcctatatcg accaggccaa tatctacgcg atgttcggcc agatggaggt
     5101 gttcgccgtc aacatggccg actataccgc gctgaccgtg gtgaaaaatc tgccgaaggg
     5161 cattcactcc ggcaaccgct acatcaagac ttttccggca acgcctgaca atgccgcgtg
     5221 gggcgatgcc tacaaggcga agtacaacga atacccgacc aactggtcgt gggagaatgc
     5281 gaccgcgatg atgcttctgg cggaagcgtc caagaaggcc aactcagccg acggcaagaa
     5341 gatcgcggaa gccctgcgcg ggctcaccat caaatgtccg ttcggcgccg acggcaccgt
     5401 gaccatgcgc gccgaggatc agaccctgat cggctatgcg atcggctggg gcacgaccat
     5461 cccgcaggag ccctacgtgc ccaacgttca ggccggcgac tggaagacca ttctggcgct
     5521 tgaagccgag tggaagaagg acaagggtta cgcgtaaggc ccgaatgaag cggaggctcg
     5581 ccgccgccgc ttcattcgtc ccgaattttc gcgcgggtag aaacgccggc gcgccgcaat
     5641 gggaggtttg tcttggacct cgacgcgctc gccggctgtc ttgcgagttc cgcctgtctg
     5701 gtgacgcaaa ccaccagcgg gctgatcatc ggcatgctgc tgttccttgt cgcggtcggg
     5761 ctgacgttga tcttcggcgt gctcaaggtc gtcaatttca cccacggcgc cttctacatg
     5821 ttcggcgcct atttcgcgat gacgagctat caggcgaccg gcagcttcgt gctggcgatt
     5881 gtttgcggcg ccgccggcac cgcgatcctg gggctcatct tcgagcgcgt cttcatgagc
     5941 cgcgtctatg gcgccaacgt gttgatgcag cttctggtct gctacgcctt cgtgctgatc
     6001 ttcgacgacg tggtccgtct cgtgtggggg ccggagttca agtcgatggg catgccagcc
     6061 gcgttccagg tgatgccgtt gttcatcgcg ggcggcgtcg tgccgccgta ttatctgctt
     6121 ctgatcggcg tggcgctggc cgctgccatc gtgctcggcg tgggccttgc gcgcacgcgc
     6181 ctcggcaagg tgatccgcgc cgccgctgat aatccgggca tggtgtccgc gctcggcatc
     6241 aataccggcg cactctatgg cgctgtgttc gcactcggct gcatgctggc ggggcttgcc
     6301 ggcgcactgg cagcgccggt gcgctcgctg accccgggca tgggcttttc cgtgctgatc
     6361 gagtccttca tcgtcaccgt catcggcggc atgggctcga tatcgggcgc gctgatcggc
     6421 gcgctcctgc tcggcctgat ccgctcgttc ggctcgcagg gctttccgct gttcaccgac
     6481 ggcctgatgt atctcttcat ggtgatcgtg ctggtggcgc gcccgaccgg cctgttcggc
     6541 aaggaggtcg catgacggaa ttgcaggcgg agcgcgcgac caaaacgctg acgacaccac
     6601 gtgacgatgc cgcgtcgaaa ggctggcgct atctcgacgt tttgattgcg cttgcggcct
     6661 ttgccgtgct ggcaagcctg ccgtttctct ccagcagcaa ggcgctgctc gacttcgtca
     6721 tccgctgctc ggcctacggc ctgttcgcca cctcgctcaa tcttctggtc ggttataccg
     6781 gcctgacttc gttcggtcac ggcatgttct tcggcctcgg cgcctacagc ttcgggctga
     6841 tcatgcagcg caccggcgcg tcgatcccgc ttgcctttgt cgcgacgctc gcgatcaccg
     6901 ccatcgttgc gaccgtcatc ggcgcgatct gcgtccgcct gaaggagatc tatttcgcct
     6961 tcgtcacgct ggcgttccag atgctgatct actcgaccat tttgtcctgg ccgtctttca
     7021 ccggcggcga tcagggcctg cgcggtggca tcccgcgccc cgtcttcatc ggcatcgatc
     7081 tgtcgaacca tgtgcagctc tatctcttta gctgcgcgct gctggtgctc gggcttctgg
     7141 caatgcgcca gatcgtgcaa tcgccgttcg gctatacgct gcgcatgatc cgcgacaatg
     7201 caaagcgcgc cgccttcatc ggtattcatg tctggcgggc caagctcgcc atcttcgtga
     7261 tggcggcgct gttcgccgcg atcggcggca tggtgatggc gcttttcgtc tccggggcct
     7321 atcccgagtt cgcctactgg acgatctcgg gcgagggcat cttcatcaac atgctgggcg
     7381 gcgttacgac cttcctcggg ccgatggtcg gcacggtgct gctgctgatc ctcaacgata
     7441 ccgtcacccg gttcaccgac taccacggca tcgtgctcgg catcgtcatc cttttcttcg
     7501 cgctcgggct gcgcaagggg ctgatggatt tcgtcgtcga ctgggccaag cagcgccgtg
     7561 aaaagggcgg gagcgcgtga ccatgctcga gatccgcggg ttgtcgaaat cctttggcgg
     7621 cgtcagggca accgacaacg tcacgctcga ttttgccgat ggttcgctca ctgcagtgat
     7681 cgggccaaac ggcgcaggca agagcacgtt cttcaatctg atcaccggcg cgttcaggcc
     7741 ggactctgga aaaattctgc ttgacggcta cgatctagca ggaagcgcgc ccgccgagat
     7801 cgtgcgtcac ggcatcgggc gcgccttcca ggtcgcgagc gttttcccgt cgttgacggt
     7861 cgaggagacc atgctggcgg cggtgggcgc ggaccagcgc cgcgccggcg tgctgcatcg
     7921 gcgttttccg ttgccagaga tgcgcgaccg ggccgagcat gcgatggaat tgctgggcct
     7981 cgccggcaag cgaaaccgcg ttgcctcgac gttgtcgcat ggcgaccaga aattgctcga
     8041 tattgcgctg gcgctggtgc tcgatcccaa ggtgctgctg ctcgacgagc cgaccgcggg
     8101 catgggcccg gaggaacgct ggcgcatgat cgacaaggtc cgcgagctct gggacacgca
     8161 aaagatcacg gtcgtcttca tcgagcacga catggatatc gtgttcaaga tcgcgcctga
     8221 gatcgtcgtc ctgtgctacg gccgcattct tgcgagcggt acgcccgcgg cgatccgcag
     8281 caacgccgcc gttatcgagg cctatctcgg caccgagcac gccggaggtt ccgcatgagc
     8341 ggacaggcaa tcatggagct tgcggatctc gacgtttatt acggcaccag ccagatcctg
     8401 ttcggcgtcg gcctgtccgt gcggcagggc gagaccatgg ccttgctcgg ccgcaacggc
     8461 gccggcaagt ccaccaccat gaaggccatc atggggcttg cgccgccgcg ccgcggcacc
     8521 gtcagcctgc gcggcgaggc gatcgcgggc ttgaagccgc atcgtattgc ccgcgccggc
     8581 ctcggcttcg tgccggaaga ccgccagatc tttccggacc acacggtgga agacaatctc
     8641 ctggtcgggc ggaaaaaggg accgcggggc gaggacgatt ggccgctcgc gcggatctac
     8701 caggtgtttc cgctgctgga gccgctgcgg cacagaatcg cgggacggtt gtccggcggc
     8761 gagcagcaga tgcttgcgat cgcgcgtacg ctgatgggca acccaaccct cctgctgctg
     8821 gacgagccga gcgaggggct cgcgccgatc atcgtgcagc gaatcggcga actcctgcgg
     8881 cagcttcgcg cggctggcgc gacggtgctg atcgccgagc agaacctgca tttctgcctg
     8941 ggcgtcgcga gccacgccac cgtcatcgac aagggccaga ttgttcactc ctcgacgatc
     9001 gaggagttga aggcaaacga cgacatccgc cggcgatacc tcgcgttgta gccacggtta
     9061 ccgcgctctt cccacgccgc ccgcgatacg cggggggagg gacgacgtcc gtcagcaagc
     9121 gctcgtgatt gcaattgcga cgaatgcaga catcggttcg cgtgccgcaa cgttttaccc
     9181 gcgcagcgcg ttctccggga ttgcccgccg cacgacctcg cgcatggcaa agctcgaatg
     9241 aatgcgggca acgcccggca tccgcgagag gtgctgcttg tggatgcgtt cgaagtcggc
     9301 gatgtcgcgc gcgagcactt gcagatgata atcgtcgctg cccgacatca ggtggcacga
     9361 cacgatgtcg gggcagcgcg cgatcgcagc ctcgaaggcc gcgagataat cctcgctctg
     9421 cttctccagc gtgatggtga cgacaaccgt ggtaaccagc ccgagcgcgg tcggttcgag
     9481 atcggcgcgg tagccgcgaa tgatgccgcc ttcttcgaga tggcggatgc gccgtgcgca
     9541 ggcggtcggc gacaacccga ccttctcggc gagctcgagc tggctggcgc gcgcgtcgcc
     9601 catgagttcg gcgaggattt tgaggtcgat gcgatcaaga tcggccacgc cgtttttctc
     9661 caagacttgc ctcatgtgcg aagattatgg gcgcaaacgg tcagccgcaa gacagatttc
     9721 gctgagaaat cgcgtgcccc taagattacc ctttattcgc cgggggaagc tgattcgagg
     9781 aagcgaacat gcgcataggg gtgccgaaag agatcaaggt cgaggaatac cgggtcgggc
     9841 tgacgccggc ctcggtgcgg gaatatgtgg cgcgcgggca tgaggtcgtg gtcgagcacg
     9901 gcgcgggtca aggcatcggc gcgggtgatg acgtttatcg cgcggccggt gcggtggtcg
     9961 ccgagactgc cgaagaggtt tttgcgacgg ccgagatgat cgtaaaggtg aaggagccgc
    10021 aggcgaccga gtgggtgcgg ctgcgcgaga atcagatcct ctttacctat ttgcacctgg
    10081 cgcccgattc cgcgcaaacc aagggcctgc tcgcatccgg ctgcaccgcg atcgcctatg
    10141 aaacggtgac cgacgcgcat ggcggcttgc cgctgctcgc gccgatgagc gaggtcgccg
    10201 ggcgccttgc gatcgaggcg gcgggtgcgg cgctgcgcaa gaccacgggc ggcatgggca
    10261 agctgatcgg cggtgtgccc ggcgttttgc cggccaggat ttcggtcatc ggtggcggcg
    10321 tggttggaac gcatgcggcg cgcatggccg ttggcctcgg cggcgacgtc accattctcg
    10381 atcgttcact gccgcggctt cgcgcgctcg atgaactgtt cggcggtcgc gtgcgcacgc
    10441 gctattcgac gctcgaagcg atcgatgagg aggtcgccgg agccgatgtc gtcatcggcg
    10501 cggtgctggt gcccggggcg gccgcgccga agctcgtgac ccgtgcgcat ctcgccacca
    10561 tgaagccccg cgccgtgctg gttgacgtgg cgatcgatca aggcggctgc ttcgagacct
    10621 cacggccgac cacgcatcag gcgccgacct atctggtgga cgatgtcgtg cactattgcg
    10681 tcgcgaacat gccgggtgcc gttccggtca cctcgagcta cgcgctgaac catgccaccc
    10741 tcccgttcgg cctggcgctt gccgccaagg ggctgcgcgc gctggtcgag gatccgcatc
    10801 tgcgcgccgg cctcaacatc catcgcgggc agatcaccaa tcgtgcggtg gcggaaagcc
    10861 agaatgcggc ggcggttgcg cccgaggctg cgcttgcggc gtgagccttg gccgcatcgc
    10921 tctactgtcc gacgaagctc ggcttgcgct tttcgacgaa ggcgcgcacc gcttccttgt
    10981 gatcggtcgt gcgcgcggcc cgtaccagat tttccgcttc ctggtccatc gaatcaagca
    11041 agctcgatgt cagcgcctga tcgagattgt ccttgatgcg gccgagcgcg aaagtcggtc
    11101 cctcagcaag ctgcctggcg agcgcaaagg cagcctcgcg cagttcgttg tccggcacga
    11161 cgcgattgat cagtccgatg gcctcacacc gtcgcgcatc gagccgttcc gacaggaaca
    11221 tcaactcgcg ggctcgcgcc gtgccggcaa ggcgggtcaa caaccacgcg attccgtagt
    11281 cgccggtcag gccgatgcgg gcatagccgg tcgacatgaa tgccgatacc gcggcgatcc
    11341 tgatatcgca ggccagcgcg atcgccatgc cggcgccggc ggcgggtcct ggaagcgcgg
    11401 caatggtagg ctttcgaacc gcgacgagcg cgcccgtcag cgtgcgctgc ttggttctga
    11461 gattggcgac tcgatcttcg aatgacgtct cagcctttgg cgcgctgtcg cccatgccct
    11521 tgacgtcgcc gcccgcgcag aacgcattcc cgctcccggt aatgaggatc gatccgacgc
    11581 gatcatcctc gccacattgc ttgatcatcc gccgcaatgc cggtgtcaga tggtcggaca
    11641 gcgcgttcct tgcctccggc cggttcagcg tgatgatcgc caccttgtcc gcgatctcgc
    11701 agagcagctc atccgtcccc gtatcaatag attgcctgga cattgatttc gatcctcatc
    11761 ctgctcgcta agaatgatga acctcatact acatccgtga ttgggcagca atcaaaagcg
    11821 agcgactgcc gctgccagcg gctaaatacc taaaaagaac ggtacctatc gcgcaagagg
    11881 cgacccatag cttgtctaac aaaagcgctt aaaagaatca tcgccgcgcc agcttgacga
    11941 gttctaaaaa agaactataa gtcgcaaccc ggcgcacgcg gttcgccgct gatccgcgcc
    12001 aacgacaaag cgccgcgcca ttcgcaggcg gggccgaaaa cgatcgtgga ggaggctcta
    12061 tgatgagagc attgtgggtt gcgtgcgcgc tcttgctcgc actggaaacg ccatcgctcg
    12121 cagcggatac gcccggtgtt acgccgaccg agatcaaggt gggtggaatc ttcccgttca
    12181 gcgggcctgc ctcgtcgatc gggctcgtag gtcgcggcgt cctcgcttac gtccaatcca
    12241 tcaacgatcg cggcggcatc aacgggcgca agatcaacta catcgcctat gacgatgctt
    12301 atagtccgcc caaggccgtc gagcatgccc gcaagcttgt cgaggccgac gaggtggcgt
    12361 tcatattcag tcagcttggc acgcccggca acacggccac agcaaaatat ctgaagtcga
    12421 agggggtgcc gacgatcgca atcgtctccg gctcgaacaa gttcaccgat gtggcgaact
    12481 ttccgctgac gaccacgagt ctcgtgagct tcgataccga gggcaggatc tatgcgaagt
    12541 ttttgaaccg gacgcttccc gatgcgaaat atgcgatcct gtatcagaac gacgacctcg
    12601 ggaaagacta tgtcaacgcc ttcagggagc tgttgaagag cgatttcgac aggaaggtcg
    12661 ttgccgccgc gtatgagatc agcgatccca ccatcgaatc gcagatcgtc aatttgaaga
    12721 gttccggcgc cggcgcactg ctggtggcgg gcacgccgaa atttgcggcg caggccattc
    12781 gcaaggcaca tgagctcggc tggaagccga tgatcctcct gaattatccg tcaagttcgg
    12841 tcggcgctac gctgaagccg gcgggtctcg acgtatcgac cggcgtcatt gtcggaacgg
    12901 tcaccatgga tcctgcggac tcgcagtggg atcagaacga gggaatgaag aatttccgta
    12961 ccttcgtcga taagtacatg cccggtgtcg acatcgccga caccaattac ctgttcggct
    13021 acacgcaggg catgctgctt gagcaactca tcaagcagtg cggcgacgac ctctcgcgcg
    13081 aaaacattat caagcagtcg agaagcttcc acgatctggt gctcccgacg gtgttgccgg
    13141 ggatcaagat caacacgtca aacaagatca acatgaacta cacgcagatg cgattgcagc
    13201 gttggaccgg aacccattgg gacctgttca acgaagtcct cgacgcgtcg tccgagtagc
    13261 gatcaccggc gtttcaatgt ttttctcagg aggttcaatg caccagacgg agccgaccat
    13321 cgaggagatc acggcctcac tgccgcggcg cctccatgaa gtagcgcaac gatacgttgt
    13381 cgacgcgcct gaccgcgctg caatgattga ggatggcgca aaatggactt accgcgatct
    13441 tgatcgctac gtccgtcaga ctgccgagcg cctcaccgcg tttggcgtca gagccgggga
    13501 ccggatcatg ctcgtcagtg aaaactgcat cgcgctggcg gcgctgctgc tggcggcgag
    13561 caggatcgat gcctgggcca tcgtctccaa tccaagacta tcggcgcgcg agctcgacca
    13621 gatccgcgat cacagcggcg cgcggcgggt gttcttcacc tgcgccgtct cgaaagaagc
    13681 cgccgctcac gcggcgcgtt acggcgcaga gattttgtcg ttcgggccgc tcagtgatat
    13741 cggtgtcggt ccgttgaatg aggctgctgc ggccgagccg gtcgaattcg actcggccag
    13801 acaggtggcc gtcctgatct acacctcggg caccacgggc aagccgaagg gcgtgatgct
    13861 ttcccatcgg aatcttcttg tcagcgccgg gaccacggcc ctgttccggc ggatgaccga
    13921 gcaggacaag gtctatgtcg tgctgccgat ctcgcacatc gtcggcatat cgcttctgat
    13981 catgacgctc atggtcggcg gcagcgtgcg acttgtaagc aagtacgacc cggccgcgct
    14041 cgcaaaggcg atggcggaag agggcattac aatcctcaac ggcgtgccag cgacctacca
    14101 gcgtttgctg gaatacaaga ccgtctcggg catgaaacgg ctcgagcgag gcgcgttgcg
    14161 atacatggga gtggcagggg cgccgctcga tctcgaattg aaggcccgcg ccgaaaatga
    14221 gatcggcctg ccactgtcga acgcctacgg catcaccgag tgctccccgg gaatatcggg
    14281 tgtgcgcctc gatgcaccgc gtagcgacga ggcggtggga acgctggtgc ctggcgtgga
    14341 ggcgcgcgtt cgcacgcttg acggcattcc ggtcggcaac ggcgagatcg gcgagcttca
    14401 cgtgcgcggc cccaatgtca tgctgggcta ttatcgcgag cccgcactca ccgcgaacgt
    14461 gatcgacagc gagggatggt tcaacaccgg cgatctcgcg cgcttcgaag gcgacgttct
    14521 ttatatcgtc ggtcgcacca aggagatgat catccgctcg ggcttcaacg tctatcccgc
    14581 ggaaatcgag gctgtgctga attcacacaa ggacgtcgtt cagtcggcag tggttggacg
    14641 caaggccgag ggcaacgagg aagtcatagc ctttgtgcaa ctgatctcgg gctcgcgcat
    14701 cggtgcgacg gagttgatga actacgttgc gcctcagctc accgcctaca agcggccgtc
    14761 gcaaatcatc gtgctcgatg ctttgccggc aacgtcgacc ggcaagattc tcaaacacag
    14821 tctcgcggag agcctgaggg tgcctgctgc cgtcagctag ccgatgaacc ttcgcggagt
    14881 gagcatcgcc ggccgccgcc tcgttgggcg atgccgatca ggccgcgtgc gtccgcgact
    14941 tcggtttctc ggcgccggct gcgccgagct tcgaaggatc gggacgtgcc cccgggcctg
    15001 gacgtgtggt gacttccttc gccgacagcg gttcgccgca ttcggagcac accatcaccg
    15061 gatcgaacag cttgccgcac ttcttgtgct catgcagaag cggccggccg cgctcgtcga
    15121 ccatgtgcgt gtcgccccag tgcacgatcg ccatcatgat cggatagaga tctaggccct
    15181 tttgggtgag gatatattcg tgtcgttttg gcgattcctg gtagggaatg cgacgcagga
    15241 tgcccagtct gaccagcttt ttcagccgtt ctgcgagcaa atggcgggta atgccgagcg
    15301 ccgactggaa cccttcgaac cgccgcgtgc gcagaaagca ctcgcgcagg atcagaagcg
    15361 tccagcggtc gccgatcacc ccgatggtcc gggccaccga gcacggctcc tcttccaagg
    15421 catcccactt cattcgaatc tccaacctga cgcgcctggg cccttcgggc cactgcagtc
    15481 aatttgcctg cattctaaat aggtactgat ggtaaaacaa cagcttcggc tcgaatcaaa
    15541 agcagtatat atcgcgcgga aaggctcggt ggaattgaca gttcaatttt agaactgtat
    15601 gagaatggtg cgcgggaagc attcgcgggc tgcgcatggt gatgcaacgt gcacgaggag
    15661 agtcccttgg caaagccctt gagggttgaa ttccacttcg atttcggcag cccgaacgcc
    15721 tatctggccg agctcgtgtt gcccgagatt gagcggcgga ccggcgtgaa gtttgactac
    15781 gttccagtgt tgctgggcgg cgtttacaag gccacgggca acatgtcgcc attcgattcg
    15841 ctgcgcggca tcaagaacaa gccggaatat aacgcgctgg agacgcagcg cttcctgcgg
    15901 cggcacaaca tcacgaagtt ccagtccaac ccgttctttc ccgtgaacac gctgacgctg
    15961 atgcgtggcg ccgtcgccgc gcagttcgag ggcgtgttcg agagctactt ccgtgcggcc
    16021 tatcatcaca tgtgggtcga gccgaagaag atggacgatc cggcagtgtt ccgcgaggcg
    16081 ttcctctcgt caggcatcga catcgaccgt ctcattgcgc gcgcacagca ggaggacgtc
    16141 aagaagaagc tgatcgagaa caccagcgac gcggtggctc gcggctcctt tggttcgccg
    16201 accttcttcg tcggcaacga gatcttcttt ggcaaggaca gtctgcgcga cgtggaggaa
    16261 gagatcgtcg cgcagttgaa tgtcgcgcgg cgcaagacgg cctgaaaatg tcccgatagg
    16321 cggacaccag cgagaggccg acgaaagcgg caatcggaga gtgggaggga tcattggtga
    16381 catcggagca agcagagcgg ggagccgcgg cggccagcat tctcgcaagt ctgccaaatc
    16441 gcatccatca ggtgctcgat caccacgtcg cagcgacgcc cgatagcgtc gcgcttgtcg
    16501 aggacggcga gagcctgacc tatcgtcagt tcgatcgagc ggtgtccgca acgaccgatg
    16561 cgctgcggga gttgggggtc cgtccgggcg accgcgtcgt gatcgtcagt gaaaactgca
    16621 taccgctggc ctgcctgctg ttcgccgcca gccggctcga cgcatgggcc atcgtcgcca
    16681 atcctcgtct gtcaccgcgc gagctcaacc agatcacgga gcatagcggc gcgcgccgcg
    16741 ttttctttac cgccgacgtc tccgaagagg cggccgccca tgcaatgcgc ctgggagcca
    16801 cgatcgccga catcggcccg ctgtccggca tcggcttttc cgcgctcaac gtggacacta
    16861 ttgccgagcc cgtcgaggcc gatggagcga agcaggttgc ggtcctgatc tacacgtctg
    16921 ggacgacggg gacgccgaaa ggcgtcatgc tcacccatcg caatcttctg ttcagcgcca
    16981 tgaccacggc ccttcaccgc gatatgaccg cggcggacaa tcagtattgc gtgctgccgg
    17041 tctctcatat cgtcggcatt tcgcttctga tcatgacgct catggtcggc gccagggttc
    17101 gcatggtcgt caaatacagc cccgcggcgc tcgtgaaggc aatggcggag gaggggatca
    17161 cgctgctgaa cggcgtgccc gcgacctatc agcggctcct cgagtacaag gcgacggcgg
    17221 ggctgcccaa gctggaacgc ggcgcgctcc gccttatcgg ggtcgccggc gctccgttgg
    17281 accttgatct caaggcacgg gtggaaaagg agcttggcct gcccttgtcc aacggtttcg
    17341 gtatcaccga gtgttcgccg ggaatctcgg gcgtgcgttc ggatgcgccg cgcgacgaca
    17401 acgcggtcgg aaggcttctg ccgggcctgg aggcgcgcat cgtcaggcgc gacggcacca
    17461 tcgtcgcgaa tggcgacatc ggtgaactcc acgtgcgcgg tccgaacgtg atgcgcggtt
    17521 actaccgtgc gcccgatctc accgcgaagg ccatcgataa ggacggctgg ttcaatacgg
    17581 gcgacctcgc ccgcttcgag gaggacgtcc ttttcatcgt cggacggacc aaggagatga
    17641 tcatccgctc cggcttcaat gtctatccgg ccgagatcga ggccgtgatc agcacccatc
    17701 cggccgtggt gcagtccgcc gtggtcgg
//
LOCUS       DATLJT010000501         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4394462,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000501 DATLJT010000000
VERSION     DATLJT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4394462"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VM910_11605"
     CDS             complement(<1..693)
                     /locus_tag="VM910_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI63212.1"
                     /translation="MRAKMPMLTGYRVLDIAQFVAGPTCTRILAELGAEVIKVELAPH
                     GDRGRASGDKPRDAKFKTSSQSTYFFQHNHSKKSLAVDIKQARGRELIRAIVPKIDVL
                     VENFAPGVMTRAGLGYDELSAIHPGLIMCSISLAGQSGPLSRKPGFDYMGAAYAGITS
                     LIGEADRGPAQFATAIGDHATGVTAAMSIGFALLHRERTGEGQYIECSLVDTYFNMHE
                     VNIPKSSLRGASF"
     gene            complement(794..1420)
                     /locus_tag="VM910_11610"
     CDS             complement(794..1420)
                     /locus_tag="VM910_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI63213.1"
                     /translation="MPEQTGEKAEVKMYSDFKSPYAYLAFDPGMTLGQRFNVRVRWIP
                     FQLRLKGKGERSVYSEYKVKYSYMDARRWAKPRGLWIRGPLKIYDTTPAAIGGLFAET
                     QGRLLDFGRTAFKKFFLREFEADQPEAVARLIADIGLSDRHYLEYLAGEGREAYDRCQ
                     AEAAADHVFGVPFFLFAGEPFWGYDRLALLEQRLEEAGLAIGDKVTAA"
     gene            complement(1539..1703)
                     /locus_tag="VM910_11615"
     CDS             complement(1539..1703)
                     /locus_tag="VM910_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63214.1"
                     /translation="MGQAGFAQARQIFTDPTDFFRPFRFEGSREPRRRSLRVLAQRLG
                     CNDVPLDRLP"
BASE COUNT          387 a          637 c          628 g          386 t
ORIGIN      
        1 aaacgaagct ccgcgcagcg acgatttcgg aatgttcacc tcgtgcatgt tgaagtaggt
       61 atcgacgagc gagcactcga tgtattgacc ctcgccggta cgctcgcgat gcagcagggc
      121 gaaaccgatg gacatcgccg ccgtcacccc cgttgcgtgg tcgccgatgg cggttgcgaa
      181 ttgtgccgga ccacggtcgg cttcgccgat cagcgacgta atgcccgcat aggccgcgcc
      241 catgtagtcg aagccgggct ttcggctgag cggtccgctt tgcccggcca aggaaatcga
      301 gcacatgatg aggcccggat gaatggcgct caattcgtca tagccgaggc ccgctcgcgt
      361 catgacgccg ggggcgaagt tctcaaccag cacatcgatc ttcggtacga tcgcccggat
      421 gagctcgcgg ccgcgtgctt gcttgatatc gacggccagg ctttttttcg agtgattgtg
      481 ctggaaaaaa taggtgcttt gcgaggacgt cttgaatttc gcatcgcgcg gtttgtcacc
      541 ggaggcacgg ccgcggtcgc catgtggcgc gagctccacc ttgatgacct cggcgcccag
      601 ctcggccaat atccgcgtgc aggtcgggcc cgcgacgaac tgggcgatgt cgagcacgcg
      661 atagcccgtc aacatcggca ttttggcgcg catggctttc tcgctgcgtt gatccgaaaa
      721 gaaaaaccga aaagaaaact catgtccgcg acgcacccct ctgcggcggg cgccagcggc
      781 gtgcgaacgt cgctcaagcc gccgtcacct tgtcgccgat tgcgaggccg gcctcttcga
      841 gccgctgttc gagcagcgca agccggtcat aaccccagaa cggttcgccc gcgaacagga
      901 agaaaggcac gccgaacacg tgatccgcgg cggcctccgc ctgacagcgg tcgtaagcct
      961 cacggccttc cccggccaga tattcgagat agtgccgatc ggacaggcca atatccgcaa
     1021 tcagtcgagc cacggcttcc ggctgatcgg cttcgaactc gcgaagaaag aatttcttga
     1081 aggctgtgcg gccaaagtcg agcaatcggc cctgcgtctc ggcgaatagt ccgccgatcg
     1141 cggcgggggt cgtgtcgtaa attttcagcg ggccgcggat ccaaagaccg cgcggtttcg
     1201 cccagcgccg agcgtccatg taggagtatt tgaccttgta ctcggaataa acgctgcgtt
     1261 cgcccttgcc tttaagacgc agctggaacg gaatccatcg tacccgcaca ttgaaacgct
     1321 gcccgagcgt catgcccgga tcgaacgcca agtaggcata cggactcttg aagtccgagt
     1381 acatcttcac ttctgctttc tcgcccgtct gctccggcat cgtgatctcc tgtcgagcga
     1441 tccgtcggct gccaatcata tctgacaacg gcgtcggccg gatgcggcag aaactcgggg
     1501 cccaaggcac cgtccccgcc gttcgaccgc acccatggtt atggcaaccg atcgagcggg
     1561 acgtcgttgc agcctagtcg ctgcgccaat acgcgcaacg accgtcgccg cggttcacgg
     1621 cttccctcga agcgaaacgg gcggaagaag tcggtcggat cggtgaagat ctggcgcgcc
     1681 tgtgcaaagc cggcctggcc caccttgcgg cctcccagat gaacgaccgc tgactggatg
     1741 gatcaatcga ggagaacgcg caagtggcct tggcacgagg gacgtcattt gcccgagtct
     1801 gggtgatcat ctcggcgcag ggttctgcct gcgagggggg tgcgcggttt ctgccgtccg
     1861 agccgcgacg tcagcgcggt tcttgcgcga cggccttacc atgtgtgccg ctctctcgat
     1921 ccggggttgc cgcatcggcg atcgcatccg ccagcgcttt ggcgaaacag ctgtagctcc
     1981 agtcgttcat atgcaggcca tccggcgaga gagatacctc gaacgggatg ccgtcgac
//
LOCUS       DATLJT010000502         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1178656,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000502 DATLJT010000000
VERSION     DATLJT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178656"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..172
                     /locus_tag="VM910_11620"
                     /pseudo
     CDS             1..172
                     /locus_tag="VM910_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            352..1566
                     /locus_tag="VM910_11625"
     CDS             352..1566
                     /locus_tag="VM910_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI63215.1"
                     /translation="MASKRLTAISVAQVRPRGKRYEIADAGCVGLYLQVYPSGAKRFA
                     VRYRLAGRPAKYTLAAGLGLAAARTAASEIRQKVEQGIDPSGEKKAARAAAEIAAGTT
                     VRAVAEAYLRQQEAKPGGERLRTIGQRRATCERLIFPNIGARPIGELRRGEIIKLLDA
                     VEIERGGRMADEVLSCLKIVFSWHALRDEKFRTPIIKGMARTRPKDRMRSRTLTDDEV
                     RRVWKACDSAGVFGDYVKFMVLTTTRRNEAARITRSEVSGADWLIAANRYKSKRPHLI
                     PLSKAAQALLAKQPQVVDCPYFFTADGRRAVRGFGPGKARLDQLSGVVSWRLHDLRRV
                     GRSLLSRIGISNDVAEMCLGHTLPTIRKTYDLHDHYREKKHAFEALAAQLDRIVHPPG
                     GKVVEGRFGRSP"
     gene            1563..>2000
                     /locus_tag="VM910_11630"
     CDS             1563..>2000
                     /locus_tag="VM910_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63216.1"
                     /translation="MTRIRRPTRKRREVTPPTQVVRPPFFVNENKFFAPLVQQVPTET
                     RMLSDEVTGAEIKNVLQAGAKAVRFGGKPTTMGYSVDAWAAIQERAAEKIPTKRRTLD
                     GDNKTATNRRHQLWQDEADKIWKNEPDLTKQQVSERIVELKSDP"
BASE COUNT          418 a          597 c          676 g          309 t
ORIGIN      
        1 catgggcgag ctgatcgcgc tcgccaaggc caatccgggc aagctcaatt ttgcctcctc
       61 cggccacggc ttggccgcgc atctcgccgg cgagctgttc aaggccgagg ccaagatcga
      121 catcgtccac gtgccctaca aaggagcggc gccggcgttg caggacgtga tcgccggcca
      181 tgtgataggt ggcggcacgt gaggacgcga aatgacattt aggtggggtt gcttgctgat
      241 ttcccttgct gcgccagcga caagagacga cactgtcgga caggcggggt aacgaccaga
      301 caggctaatc cgttacccgc cgaagggagc ggagtaacgg ggtaaccatt catggccagc
      361 aagagattga cggcgatcag cgtcgcccag gtccgaccac gcgggaagcg atatgagatc
      421 gcggacgccg gatgcgtcgg gctttacctt caagtttatc cgagcggcgc gaagcgcttt
      481 gccgtccgct atcggctcgc gggcaggcct gcgaagtaca cgctcgctgc cggtcttggt
      541 ctggcggcgg cgcgcacggc cgccagcgag atacggcaaa aagtcgagca gggcatcgac
      601 ccgagcgggg agaagaaggc tgcgagggcc gcagccgaga tcgcggctgg caccaccgtg
      661 cgcgccgtcg ccgaagccta cttgcggcag caggaggcca agccgggagg cgagaggttg
      721 aggaccatcg gccagcgccg cgcgacgtgc gaaaggctga ttttccccaa catcggtgcc
      781 cggccgatcg gcgagctccg gcgcggtgag atcatcaagc tgctcgacgc ggttgagatc
      841 gaacgcggcg gccgcatggc cgacgaggtg ctgagctgcc tgaaaatcgt attcagctgg
      901 cacgcgctcc gggacgaaaa attccgcacg ccgatcatca aaggcatggc gcgcacccgg
      961 ccgaaggacc ggatgcgcag ccgcaccctg accgacgacg aggttcgccg cgtgtggaaa
     1021 gcctgcgaca gcgcgggcgt attcggtgac tacgtaaagt tcatggtgct taccaccacg
     1081 cgtcgtaatg aggcggcgcg gataacacgc agtgaggtga gcggcgctga ttggctcatt
     1141 gcggccaacc gatacaagag caagcgcccc catttgatcc cgttgagcaa ggccgcgcag
     1201 gcgttgctgg ccaagcagcc gcaggtcgtc gattgcccat acttcttcac tgctgacggt
     1261 agacgtgccg tgcgcggttt cggcccgggc aaggctaggc tcgaccagct gtccggcgtc
     1321 gttagttggc gcttacatga tcttcgacgc gtgggccgat ccctgttgag ccgcattggg
     1381 atcagcaacg acgttgccga aatgtgctta ggtcacacgc tccccaccat tcgcaagact
     1441 tatgacttgc acgatcatta tcgcgagaaa aaacacgcct tcgaggcgct ggcggcgcaa
     1501 ctcgaccgca tagttcatcc gccgggaggc aaggtcgtcg aagggcgctt cgggcggagc
     1561 ccatgacacg gataagaaga ccaacgcgca agcgccgtga ggtcacgccg ccgacgcagg
     1621 ttgtgcggcc gccattcttc gtcaacgaga acaaattttt cgcgccgctg gtgcagcagg
     1681 tgccgaccga aacgcggatg ctgagcgacg aggtcaccgg cgccgagatc aaaaatgtat
     1741 tgcaggccgg cgccaaggcg gtgcggttcg gcggcaagcc gacgaccatg ggctatagcg
     1801 tcgacgcgtg ggcggcgatc caggagcggg ccgccgagaa gataccgacc aagcggagga
     1861 cgttggacgg cgacaacaag acggcaacga acaggcgtca ccagctttgg caggacgagg
     1921 ccgacaagat ttggaagaac gagccggacc tgacgaagca gcaggtgtcg gagcgcatcg
     1981 tcgagctcaa gagcgacccg
//
LOCUS       DATLJT010000503         4067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_489109,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000503 DATLJT010000000
VERSION     DATLJT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489109"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VM910_11635"
     CDS             complement(<1..1013)
                     /locus_tag="VM910_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI63217.1"
                     /translation="MRIAVLGGGPGGLYFAYLWKTRDPESHVDLFEQNAAGATWGFGV
                     VFSDEALDFLRADDPEVVDAITPCMESWQNITLNLRGENVEIDGVGFSSIGRIELLKI
                     LQARAHDAGVAAHYETIVQSVDQLIDYDLIVAADGLNSLVRRSFEGDFGTSLSYSSNK
                     FAWYGTSKRFETLSQTFVATERGAFNAHHYRYSPSMSTFLIECDRATWQAYGFAYKTV
                     DESKAICEDVFAEALGERLLVSNKSVWRNFPWVWNEHWSYKNMVLIGDALHSAHFSIG
                     SGTRLAIEDAIALARALEAEAHVATALARYEAERKPVVKKLVTAARTSADWYEHFAEH
                     MKL"
     gene            complement(1188..2045)
                     /locus_tag="VM910_11640"
     CDS             complement(1188..2045)
                     /locus_tag="VM910_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HVI63218.1"
                     /translation="MIAIVIRAATPAALLAFTAVSIATSALAQAPTEAQREAIKSQCR
                     SDYIAHCSSIPPGGAASLECLQKNMASLSAGCAGAVRAVEAPAETKAEPRGETKAEPK
                     EETKVEPKEEPKPAPATTTARPAAGPAPKAASAPAKQRPTSAQISAIRSACRSDYPKV
                     CAGVPTGGAPALQCLEKNEARLSASCRKAVAAVSGGVAPSAAGGAAPATAGATPTATP
                     AAAAPMLALRPLRPREVLFVMRSACGEDVQALCPGVAPGGGRIVECLVAQSAKLSPAC
                     RGVLDQFAQ"
     gene            complement(2121..3119)
                     /locus_tag="VM910_11645"
     CDS             complement(2121..3119)
                     /locus_tag="VM910_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVI63219.1"
                     /translation="MASVNRQILLVEKPSGKLGPEHFRLTDGPIPEPKDGEALLRVRC
                     ISLDAANRAWMHGATYRSAVEANTVMAGGGIAEVVASKSPGLAPGDIVFGDTGWQDYA
                     AIPAKHLTKLPKLEPMTHLLSVYGIAGLTAYFGLLEIGKPKAGETVVVSAAAGSVGSI
                     VGQIAKIKGCHAVGIAGGKDKCHWLTSELGFDAAVDYKDGAVFKALRAAAPKGIDVYF
                     DNVGGDIFEACLPQMNNYGRIACCGAISQYDGVPAAHGPRGVPGLIVVKRLIMQGFIV
                     MDFMKERDRALADLQSWVAGGKLKVQEDVIKGLENTPKALIGLLAGENRGKRMVRV"
     gene            3342..>4067
                     /locus_tag="VM910_11650"
     CDS             3342..>4067
                     /locus_tag="VM910_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HVI63220.1"
                     /translation="MSNRFTQYILIAMVLGIVMGALIYNFMPDSRAEIAADINLIALL
                     FLRLIKMIIAPLVFATLVGGIAHMGSGAKLGRIFAKTMGWFVSASFVSLLLGLLMVNL
                     LQPGANFPGTLPDKTQSTGLPVSAFSVEKFLTHLVPTSIADAMAQNEILQIVVFAVFF
                     SVALGSMPERAKPILSMIDDLAHVMLKVTAYVMMFAPLAVWAAITATVAKNGLLVLWK
                     LVVFMGGFYLSLMILWAILIAVGF"
BASE COUNT          723 a         1302 c         1250 g          792 t
ORIGIN      
        1 tcgagcttca tgtgctcggc gaaatgctcg taccagtcgg cgctggtgcg cgccgcggtc
       61 accagtttct tcacgaccgg cttgcgctcg gcctcgtagc gggcgagcgc ggttgcgaca
      121 tgcgcttcgg cctcgagcgc cctggcgagc gcgattgcgt cttcgatcgc tagccgcgtg
      181 ccggaaccga tcgagaaatg cgccgagtgc agggcatcgc cgatcagcac catgttcttg
      241 taagaccaat gctcgttcca gacccaggga aaattgcgcc acaccgattt gttcgacacc
      301 aaaaggcgct cgccgagcgc ttcggcaaag acgtcttcgc aaatggcctt ggattcatcg
      361 acggtcttgt aggcaaagcc gtaggcctgc caggtcgcgc gatcgcattc gatcaggaat
      421 gtgctcatcg acggcgaata gcgatagtga tgcgcgttga acgcgccgcg ctcggtcgca
      481 acaaaggtct gcgacagcgt ctcgaaccgt ttcgaagtgc cgtaccaggc gaatttgttc
      541 gaggaatagg atagcgaggt gccgaaatcg ccctcgaagc tgcgacgcac cagcgagttc
      601 agcccgtcgg ccgcgacaat caggtcgtag tcgatcaatt ggtcgaccga ttgcacgatc
      661 gtctcgtaat gcgccgccac accggcatca tgggcgcgcg cctgcaggat cttcaacagc
      721 tcgatccggc cgatcgaaga gaagccgacg ccgtcgatct cgacgttttc tccgcgcaaa
      781 ttcagggtga tgttctgcca gctctccatg cacggcgtga tcgcatcgac cacttcggga
      841 tcgtcggcgc gcagaaaatc cagcgcctcg tcggagaaca ccacgccaaa gccccaggtc
      901 gcgccggcag cgttctgttc gaacaggtca acgtgcgact cgggatcgcg ggtcttccag
      961 agataggcga aatacagccc gccggggcct ccgccgagca cggcgatgcg cacgatgacc
     1021 tcccgccaaa tagattgaca gtatactcac aatccggtcc agcctaaacg gcgcgggccg
     1081 gccgcgccag cggaaactat cggcggcaag tcagccgatc aatagcagcg cctcacacag
     1141 ggccattgac cgggaccggg cggcaaaccg gaaccggctt gcacaggcta ttgcgcgaac
     1201 tgatccagca cgccgcggca ggccggggac agcttcgccg attgcgccac caggcattcg
     1261 acgatgcggc cgccgcccgg cgcaacaccg ggacaaagag cctgcacgtc ctcgccgcag
     1321 gcagagcgca tgacgaacag cacctcgcgc ggacgcaacg gccgcaatgc cagcatcggt
     1381 gccgccgcgg caggagtagc cgtcggcgtc gcaccggccg tggcgggagc agcgccacct
     1441 gccgccgacg gtgcaacgcc gccggacaca gcggcgacgg ctttcctgca actcgccgac
     1501 agcctggcct cgttcttttc aaggcattgc aatgcgggcg cgccgccggt cggcacgccg
     1561 gcgcaaactt ttgggtaatc ggaacggcag gcgctgcgga tagccgagat ctgtgcgctg
     1621 gtcggtcgtt gcttggccgg tgcgctcgca gcttttggtg cgggcccggc ggctggcctg
     1681 gctgtcgtgg tcgcaggcgc cggcttcggc tcttccttgg gctcaacctt tgtctcttcc
     1741 ttgggttcgg cctttgtctc tcccctgggc tccgctttgg tttcggccgg cgcttccacc
     1801 gcgcgcaccg cgccggcgca gcccgcagac aggctcgcca tgttcttctg caggcattcc
     1861 agcgaggccg cgccgcccgg cgggatcgag gagcaatggg cgatgtaatc ggagcggcat
     1921 tgggatttga tcgcttcgcg ctgcgcttcg gtcggagctt gcgctaaggc cgacgtggcg
     1981 atgctcacgg cggtgaaagc gagcaacgcc gcaggcgtcg cagcacgaat cacgatggcg
     2041 atcatttgaa cctgcccttt ttgtttgttg tcgcgtcgtt tgattgtggc gacgttgcat
     2101 gacgagagcc tgacgacttg tcaaaccctg accattcgct tgccgcggtt ttcgccggcc
     2161 aaaagtccga tcaacgcctt tggggtgttt tccaggcctt tgatgacatc ctcctggacc
     2221 ttcaacttgc cgccggcgac ccaggattgc agatcggcga gcgcgcgatc ccgctccttc
     2281 atgaaatcca tgacgatgaa gccctgcatg atgagacgct ttacaacgat cagcccgggc
     2341 acgccgcggg gcccatgcgc cgcaggcacg ccgtcatatt gcgaaatcgc gccgcagcag
     2401 gcgatgcgac cgtaattgtt catttgcggc aggcaggcct cgaaaatgtc gccgccgacg
     2461 ttgtcgaaat agacgtcgat gcctttcggc gccgcggcgc gcaacgcctt gaacaccgcg
     2521 ccgtccttgt aatcgacagc ggcgtcgaag ccgagctcgg atgtcagcca gtggcacttg
     2581 tccttgccgc cggcgatccc cacggcgtgg cagcccttga tcttcgcgat ctggccgacg
     2641 atcgagccga ccgagcctgc ggcagcggat accacgacgg tctcgccggc cttcggcttg
     2701 ccgatctcca gcagtccgaa ataggcggtg aggccggcga tgccgtacac gctcaacaga
     2761 tgggtcatcg gttccagctt cggtaatttg gtgagatgtt ttgccggaat ggccgcatag
     2821 tcctgccagc cggtatcccc gaacacgatg tcgcccggcg caaggcccgg cgatttggag
     2881 gcaacgacct cggcgatgcc gccgcccgcc atcaccgtgt tggcctcgac ggccgagcga
     2941 taggtcgcac catgcatcca cgcacggttg gccgcatcga gcgagatgca gcgcacgcgc
     3001 agcagcgctt cgccatcctt cggctcgggg atcggaccgt ccgtgagcct gaaatgttcg
     3061 ggcccgagtt ttccgctcgg cttctccacc agcaaaatct gccgattgac gctcgccatg
     3121 gtgttccctc ccatcgactc tgacgattat tgatgcagcc ggcgcgcagc aaacgagtag
     3181 ccgcattcat tgtgcgttgc gttcaacgct aaccagagtg tcttcaaacc acaacgggga
     3241 atgtgctggc acaccacgct ttcacgggaa gcgtgttgcg tcgcccctga aatctgccaa
     3301 actattcgcc tgccggttga tcggacccac ggggtaagca aatgtcgaac aggttcacgc
     3361 aatacatcct gatcgcgatg gtgctcggta tcgtgatggg tgcgctgatc tacaatttca
     3421 tgcccgacag ccgggcagag atcgcggccg acatcaacct gattgctttg ctgttcctgc
     3481 gcctgatcaa gatgatcatc gcccctctgg tgtttgcgac cctcgtcggc ggcatcgctc
     3541 acatgggaag cggcgccaag ctcgggcgca tcttcgcgaa gaccatgggc tggttcgtca
     3601 gcgcgtcctt cgtctcgctg ttgctcgggc tcctgatggt caacctgctg cagcccggcg
     3661 ccaacttccc cggcaccttg cccgacaaga cccagtcgac gggtcttccg gtgtccgcct
     3721 tctcggttga gaaattcctg acccacctgg tcccgacctc gattgcagat gcgatggcgc
     3781 agaacgaaat cctgcagatc gtggtgtttg cggtgttctt ctcggtggcg ctcgggtcga
     3841 tgccggaacg cgccaagccc atcctgtcga tgatcgatga cctcgcccat gtcatgctga
     3901 aagtgaccgc ttacgtgatg atgtttgcgc cgcttgcggt gtgggcggcg atcacggcga
     3961 cggtggcgaa gaacggcctc ctcgtgctgt ggaagctcgt ggtcttcatg ggtggattct
     4021 atctgtcgct gatgatcctg tgggcgatcc tgatcgcggt cggcttc
//
LOCUS       DATLJT010000504         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1178813,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000504 DATLJT010000000
VERSION     DATLJT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178813"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            953..>2016
                     /locus_tag="VM910_11655"
     CDS             953..>2016
                     /locus_tag="VM910_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI63221.1"
                     /translation="MNRGGRDDADWSKVAIEWTPADAKVYGYKDPFGTEHLMWIIGSR
                     AVQLYFTQLRMAGAQVRKVLIANAAQKWGVDAATLKTEPSVVINPANGARLTYGEIAA
                     FGTIPATLPAVDPKELKERTDFRLIGKSVPRRDTPSKVNGTAQYAIDVKLPGMVYAST
                     LHSPVHNAQPGIWDPGKQDQSAATPETWNDAEIKAMKGVIAVVKLANGVAVVADHFET
                     AKAGRDALKVTWRKAKAEGFNSDRALEDYVKIHADPNAQAVKLEAKGDVPAAFAAAAK
                     TYHAEFRSDYGYHAQMEPLNAVVRINEAGDKAEVWEGSQAPDESRKAVAKALGFGLEQ
                     VDFHQCYMGGGFGRRSLGDYA"
BASE COUNT          382 a          615 c          666 g          353 t
ORIGIN      
        1 gataaagtct cgccgcctca tgctgagcgt cgggagtgtc tccaaccgcc ttgcgccgcg
       61 gtcggtcggc ttaccgcacg ctcaacctgc cgcagatggg ccggcaagtc cttggggcag
      121 acctgaatcg tcctgaatct aggtgaggct gccgaacccc tccctctgct gccccccgac
      181 gatagcacag acgggcgcgg caagccgctg caatgcggga tttcagtatc gtttatgtcc
      241 gcgttggggt caaaatgcga agaactcgaa ccgagcatat ctggtccgct cttgtcgaaa
      301 gcggacgcta gagcggacgt gccggttggt cgcgttcgtg ccaatatcgg tcattggcta
      361 acgcatcccg cccgagggcg gacgctcttt ttgggagcca agccgttgcg agctgcttct
      421 gggcagatcg ctttcggttt tttggaaata atcgcggatg gtcggatcgc aatctcgcac
      481 gcctgtccga tgggctctcc gggatggcgg gatgtcggca ggcggctcgc tttcggtttt
      541 tgggaaataa gacggattgg cgggtcgcga tcgctcaccg cggacccaga gacgttgagg
      601 atggtcgatg gtcggagggt tgggcggccg gagcaacgcg cgccatatgt ccccgcgcgg
      661 cttccaatgg atattcgtcg ctaggtcgcg acgcgatgcc cgctggcgcg gcagcgatta
      721 tccactgtcc acgcggccgg tcgcgatcgc cacgcacggc cgggtctccg agatggggtc
      781 ggagagtcga tggtccgacg cctgaagtgc attgcggcgg gcggtgcctg agtatggcct
      841 cggcgcctca gacctccggc gatgtctggg ctctagggct ctggcgggcc gcacgatgcg
      901 gggaccgatg aggggacgga cgaccgatca gcctgtaagc cattgaaaag acttgaatcg
      961 tggcggaaga gatgacgccg actggtcgaa ggtcgcaatc gaatggacgc cggccgacgc
     1021 caaggtttac ggctacaagg atccgttcgg caccgaacat ttgatgtgga tcatcggcag
     1081 ccgcgcggtg cagctctatt tcacccagct gcgcatggcc ggcgcccaag tgcgcaaggt
     1141 gctgatcgcc aacgccgcgc agaagtgggg cgtcgatgcc gcaacgctca agaccgagcc
     1201 gagcgtcgtc atcaaccctg ccaacggggc gcggctcacc tatggcgaga tcgctgcgtt
     1261 cggcacaatt cctgccaccc tgccggcggt cgatcccaag gaactcaagg agcgcacgga
     1321 tttccgcctg atcggcaagt cagtgccgcg acgcgacacc ccgtccaaag tcaacggcac
     1381 ggcgcaatac gccatcgacg tgaaattgcc gggcatggtc tatgcctcaa ccttgcactc
     1441 gcccgttcac aatgctcagc ccggcatttg ggatccgggc aagcaggacc aatcggcggc
     1501 tacgccggag acctggaacg acgccgagat caaggcgatg aagggcgtga tcgcggtggt
     1561 caagctcgcc aacggcgttg ccgtcgtcgc cgatcatttc gagaccgcca aggccggccg
     1621 cgacgcccta aaagtgactt ggcgaaaagc caaggcggag ggttttaact ccgaccgggc
     1681 gctcgaggac tacgtcaaga tccacgccga tccgaatgcg caagcggtca agctcgaagc
     1741 aaagggcgac gtgcccgcgg cgttcgcggc cgcggccaag acctatcacg ccgagttccg
     1801 cagcgattac ggctatcacg cccagatgga gccgcttaac gcggtggtgc gcatcaacga
     1861 agccggcgac aaggccgagg tttgggaggg cagtcaagcg cccgacgaat cccgcaaggc
     1921 cgtggcgaag gcgctcgggt tcggcctcga gcaagtggat ttccaccaat gctacatggg
     1981 tggcggcttc ggccgtcgtt cgctcggcga ctacgc
//
LOCUS       DATLJT010000505         8368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_718299,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000505 DATLJT010000000
VERSION     DATLJT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718299"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VM910_11660"
     CDS             <1..547
                     /locus_tag="VM910_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI63222.1"
                     /translation="LLGFCKPKRPDSLIGSMGFGPMGFGVAGVLGAKFAAPDRPCVSV
                     CGDGAFFMHANVLGTAVEYNLPVVWVVWNNYAYASIRGLQRGYLGGRELATDFHDPRT
                     GERYNPDFAAMARSCGIEGVRVDRAGDIGDALRRAIAANKPYLIDVDIAADVNPAGAG
                     VWELPGLGQSKAGIGTRYQPV"
     gene            565..1269
                     /locus_tag="VM910_11665"
     CDS             565..1269
                     /locus_tag="VM910_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI63223.1"
                     /translation="MSLKGKKVVVVGGSSGIGLSTAEMAKREGADVIVASRNAARLGA
                     VAEKLNAVAIAADVTDDNSVANLFRNTGPVDHVVVTAAQLRTGPFKTVSMDDVRATMD
                     SKFWGAWRVARAAEFRQGGSLTLVSGYLSIRPRQNSAIIGAANGALESLARSLALELA
                     PVRVNAVSPGIIDTPIRAAMPEEARRDMRAKTAAALPVGRVGEGEDVALQILAFMANG
                     FATGSIVYLDGGALVT"
     gene            1308..1679
                     /locus_tag="VM910_11670"
     CDS             1308..1679
                     /locus_tag="VM910_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI63224.1"
                     /translation="MASDNNGAFIRNIAEVPWREFPNHFGGALSKPLVMPETAGSQRI
                     DYRISMYQPMAYVARHKHRVQEQVYHVLDGEGLMEIGNEKRVVRKHDVIFLPPGVEHA
                     ISNTGLVDLVFLVVTSPVTDD"
     gene            complement(1705..2493)
                     /locus_tag="VM910_11675"
     CDS             complement(1705..2493)
                     /locus_tag="VM910_11675"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63225.1"
                     /translation="MAGMIGGEPFISLRHVRKVYRSGNSEFLAVSDVTMDVQEGELVS
                     LVGPSGCGKTTVLKILAGLHGADGGSVRIGNAHTAFDPARDVGMVFQQALLLKWRTIL
                     DNVLLPAEIVGLPIKTARTRARDLLNLVGLSDYEDKYPQQLSGGMQQRAAIARAFIHD
                     PKLILMDEPFGALDALTREQMNLEMLKIWRESGKTIIFVTHSIQEAVFLSSHCAVLTA
                     SPARMAEYFKIELPFPRALPIKTTDEFGVYARRIYASLGLATSG"
     gene            complement(2481..3536)
                     /locus_tag="VM910_11680"
     CDS             complement(2481..3536)
                     /locus_tag="VM910_11680"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI63226.1"
                     /translation="MHFSQIKSDVRRLIAEGTVLPAHPLALDADRNLDKVHQRALTRY
                     YIDAGSGGLAVGVHTTQFAIREVGLYRPVLELAADAASQWTKRPLALIAGLSGPTRQA
                     LAEADIARSIGYHAGLLSLAAMKSASEEEIIAHCRAVAREIPLVGFYLQPAVGGVILS
                     ANFWRRFAEIDNVIAIKIAPFNRYRTLDVLRGVFSAGALDRITLYTGNDDHILLDLTL
                     PFDLRDNGVAVRTHIRGGLLGHWSVWTASAIRQFEMCKAARNRQAVPAELLALDARVT
                     DCNSAFFDVANNFHGCIAGCHEVLRRQGLMAGIWCLDPAEGLSPGQLKEIDRVCNEHA
                     DLSDDDFVAANLQKWLG"
     gene            complement(3549..4376)
                     /locus_tag="VM910_11685"
     CDS             complement(3549..4376)
                     /locus_tag="VM910_11685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI63227.1"
                     /translation="MDATEQLAIVQLGEADTASGVLLSSEAQWNQNEADWRFFLTKGI
                     VYGVRDRDGRVIASAALLPYSDGDAWISMVLVTANWRRRGFATRLVDACIGAATRLGV
                     TAWLDATPAGAAVYGPLGFSPTIQLRRLRFERASTVKADVELAPGKLDDLLKLDIRAM
                     GFDRRDLLRELSERATSRLLSHGDAMALVRDGRTARHIGPLFAGTPTHALAMVHDIAA
                     DEKHPLLIDAVASQEIFLQGLTNGGWTIERPFQRMRFGRAGTKAAGLPFAVAGPEFG"
     gene            complement(4363..5400)
                     /locus_tag="VM910_11690"
     CDS             complement(4363..5400)
                     /locus_tag="VM910_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63228.1"
                     /translation="MLLLRDNLPETIPDIAALDELLCRPSQALIDDLGRVDGDIMILG
                     VGGKMGPTLAGLAKAALPERRIIGVARFSEAGIEPWLKARGIETIHCDLLDEAAIRRL
                     PQVTNIIFMAGRKFGAEGNLALTWAMNAHVPALVAQAFRSSRIVAFSTGCVYPFVPID
                     GAGADESLPPNPPGEYAQSCVGRERMFEYFSRAFRTPGRLFRLNYAIDMRYGVLHDIA
                     TKVRDGTPIDVTLGHVNFIWQGDASSQALRSLAHCETPTSPINVSGHEILAVRDLAAR
                     FGDLLGCRPVITGREEPTAWLTNTSLATKLFGLPIVDTEQLIKWTADWVARAMPSLGK
                     PTKYEVRDGRY"
     gene            complement(5418..6188)
                     /locus_tag="VM910_11695"
     CDS             complement(5418..6188)
                     /locus_tag="VM910_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63229.1"
                     /translation="MFGGGERSARSFAIILIVHLTVIVLWQVLVDAFHVPKFILPSPL
                     AALATLSHPNYAWAANLAVTAIEILGGFLLGAVVGVALALIFTWSRLVALLLLPLFVT
                     LNMIPKVAMGPLVIVWFSYGIIANILIAFSICFFPILLTTARGLSEVEPDLLDLVKSL
                     HGSRWILFRKIQLPGSLPYVFSGMKVGAILAVAGAIVGEFIASDRGLGYLMIQVQSSL
                     DTPAMVMAVVLLTLLGVGLYGLVLGLERLFVVRDIRLQ"
     gene            complement(6277..6924)
                     /locus_tag="VM910_11700"
     CDS             complement(6277..6924)
                     /locus_tag="VM910_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVI63230.1"
                     /translation="MPDRRANGKKPKRRNPEATRRKLLAAARREFANSGLAGARVDEI
                     ATRAGVNKQLVYHYFGDKDALYLAVLEWVYEEIRAQERKLNLTGLPPHQAIRKLIESS
                     FDHLAAHPDFILLLNDENRGGARHVRTSRRLEAMHSPLVNMVSKILSEGVRAGVFRKG
                     INPVHLYISIAGLSYFFFSNTPTLSAIFGKDLASAAARSARRKHVVDLVLQALRP"
     gene            7084..8046
                     /locus_tag="VM910_11705"
     CDS             7084..8046
                     /locus_tag="VM910_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63231.1"
                     /translation="MSCAPCRAGEAVNLLLNWTPTADHSPFYYAKAQGWYEKAGIDLT
                     IETGKGSGVSSLKVGSGGSPFGIADLATMLVARSKGADAVAVMSIYANTGQTFYWLKS
                     YGVNGPKDFPGHKIGNPPGDASRVMWPAFAKAAGIAPDSVNFVNVGPTAKIAALKSHT
                     VDIISDFYNEHDLKVIEFDGDLGYVNWKDIGLNPYGNSLIVNGAFLEKNPKLVEDFVK
                     VTQKAYAACVADVAPCLKALLDQVSGLDKENQQRQWERIKVLMTDEFTTAKALGWIDG
                     ERMKKDYELVQTYLGMEKPFDVQTAFTTRMLDPSIKMDASKVKK"
     gene            complement(8142..>8368)
                     /locus_tag="VM910_11710"
     CDS             complement(8142..>8368)
                     /locus_tag="VM910_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63232.1"
                     /translation="PWYGIYVPSATPDALVEKIHDAIDKALENPDVQRQLAISDIPGK
                     PMPLSDLTVLMKADYEKLNKVVKTSGMALQ"
BASE COUNT         1597 a         2585 c         2694 g         1492 t
ORIGIN      
        1 gctgctcggc ttctgcaagc cgaaacggcc ggactccctg atcggctcga tgggcttcgg
       61 tccgatgggg ttcggtgtcg ccggcgtgct tggcgcgaaa ttcgcagcgc ccgatcggcc
      121 ctgcgtgtcg gtgtgcggcg acggcgcctt cttcatgcat gccaatgtgc tcggcacggc
      181 cgtggaatat aatctacccg tggtctgggt agtctggaac aactacgcct atgcgtcgat
      241 ccggggcctg cagcgcggct atctcggcgg aagagagctt gccaccgact tccatgatcc
      301 cagaaccggc gagcgctaca atccggattt cgcggcgatg gcgcgctcct gcggcatcga
      361 gggcgtgcgc gtcgatcgcg ccggcgacat tggtgatgcg ctccgccggg cgatcgccgc
      421 caacaagccc tatctgatcg acgtcgatat cgccgccgac gtcaaccctg ccggcgccgg
      481 tgtgtgggaa ttgcccgggc tcgggcaaag caaggcgggt atcggcacgc gctatcagcc
      541 ggtctgatcg tgaagggagg aaacatgtcg ctcaagggca agaaggttgt tgtggtcggc
      601 ggttcgtccg gcattggcct ctcgaccgct gaaatggcaa agcgggaggg agccgatgtc
      661 atcgtcgcct cgcgcaacgc tgcgcggctt ggcgccgtgg ccgaaaagct gaacgcagtc
      721 gcgatcgcag ccgatgtcac cgacgacaac agtgtcgcga accttttccg caacacgggt
      781 ccggtcgatc atgtcgtcgt cacggccgcg cagcttcgaa ccggtccctt caagactgtc
      841 tcgatggacg acgtgcgcgc caccatggac agcaagttct ggggcgcctg gcgggttgcc
      901 cgcgccgctg agtttcgcca gggcggatcg ctcacactgg tctccggtta tttgagcatt
      961 cgaccgcgac aaaactcggc catcatcggt gcggctaacg gcgcgctgga gtcgcttgcg
     1021 cgcagcctcg cgctcgagct cgcgccggtc cgcgtcaatg cggtctcgcc cgggatcata
     1081 gatacgccga tccgcgccgc gatgccggaa gaggcgcgcc gcgatatgcg cgccaagacc
     1141 gcggcggcgc tgcccgtcgg ccgcgtcggc gagggtgagg acgtggcgct gcaaatcctc
     1201 gccttcatgg ccaacggctt tgcgaccgga tcgatcgtct atctcgacgg cggagcgctg
     1261 gtcacctgac cgccgccacc aaaacccgcg aacccgggag gagcgaaatg gcttcggaca
     1321 acaacggcgc attcatccgc aacatcgccg aggtgccgtg gcgcgaattt cccaatcatt
     1381 tcggcggcgc gttgtcaaaa ccgctggtga tgccggagac ggcgggctcg caacgcatcg
     1441 actaccgcat ctcgatgtac cagccgatgg cctatgtggc gcgccacaag cacagggttc
     1501 aggagcaggt ctaccacgtc ctcgatggcg aaggcttgat ggagatcggc aacgagaaac
     1561 gcgtcgtacg caagcacgac gtgatcttcc tgccgcccgg cgtcgagcac gcgatctcga
     1621 acacaggcct ggtcgatctg gtgtttctgg tcgtgacgtc gcccgtcacc gacgactgaa
     1681 gcgcaatggc ctccggtcga agcgctagcc gctcgtcgca agacccaggc tcgcgtagat
     1741 ccgccgcgca tagacgccga attcatcggt cgtcttgatc ggcagcgcac gcggaaacgg
     1801 cagctcgatt ttgaaatatt ccgccatgcg cgcaggcgag gcggtcagca cggcgcaatg
     1861 cgaagacagg aacaccgcct cctggatcga gtgcgtgacg aagatgatgg tcttgccgct
     1921 ctcccgccag attttcagca tttccaggtt catctgttcg cgtgtcagcg cgtccagcgc
     1981 gccgaacggc tcgtccatca ggatcagctt cggatcatgg atgaaagcgc gtgcgatcgc
     2041 cgcacgctgt tgcatgccgc cggagagctg ttgcgggtat ttgtcctcat agtccgaaag
     2101 cccgacaagg ttgagcaggt cacgcgcccg cgtgcgcgcg gtcttgatcg gcagccccac
     2161 gatttccgcc ggcagcagca cgttgtcgag aatggtgcgc catttcagga gcagcgcctg
     2221 ctggaacacc atgccgacgt cgcgcgcggg atcgaacgcg gtgtgcgcgt tgcctatcct
     2281 gacgctgccg ccgtcggcgc cgtgcagacc cgccaggatc ttgagaaccg tggttttgcc
     2341 gcaaccggac gggccgacaa gcgacaccag ttcgccctcc tgcacgtcca tggtgacgtc
     2401 ggagacggcg agaaactcgc tgttgccgga tcgatagacc ttgcgtacgt ggcgcaggct
     2461 gatgaacggt tcgcccccaa tcatcccagc catttctgca aattggcggc cacgaaatcg
     2521 tcgtcggaga gatcggcgtg ctcgttgcag acgcggtcga tctccttgag ctggccgggg
     2581 ctcaaaccct cggccgggtc gaggcaccag atgccggcca tcagcccctg acgccgcagc
     2641 acttcatggc agccggcgat gcagccgtga aaattgttgg ccacgtcgaa gaacgcactg
     2701 ttgcagtcgg tgacgcgggc atcgagcgca agcagttcgg ccggcaccgc ctgcctgttg
     2761 cgcgcagcct tgcacatctc gaactgcctg atcgctgacg cggtccagac cgaccaatga
     2821 ccgagcaggc cgccgcggat atgggtgcgg accgcaactc cgttgtcgcg cagatcgaag
     2881 ggcagcgtaa ggtcgagcag aatatgatcg tcgttgccgg tgtagagcgt gatgcggtcg
     2941 agtgcgccgg cagaaaaaac gccgcgcaat acgtcgagcg tgcggtaacg gttgaacggc
     3001 gcgatcttga tcgcaatgac attgtcgatc tcggcaaacc ggcgccagaa atttgcgctc
     3061 aggatcacgc cgccaactgc gggctgcaga tagaagccga ccagcggaat ctcccgtgcc
     3121 accgcccggc aatgggcgat gatttcctcc tcggaggccg acttcatcgc cgcgaggctg
     3181 agaaggcctg cgtggtagcc gatgctgcgg gcgatatcag cctcggcgag cgcctgcctg
     3241 gtcgggccgg agaggccggc gatcagcgcc agcggccgct tggtccattg cgatgcggcg
     3301 tcggctgcga gctccaacac cggccgatag aggccgacct cccggatcgc gaactgcgtg
     3361 gtgtggacgc cgaccgcaag cccgccgctg ccggcgtcga tataataacg cgtcaacgcg
     3421 cgctgatgga ctttgtcgag attgcggtcg gcatcgaggg cgagcggatg cgcgggcagc
     3481 accgtgccct cggcgatcag ccgacgcacg tcggatttga tctggctgaa atgcattttg
     3541 gcgcgtcctt atccgaattc agggccggca acggcgaaag gcagcccggc ggctttcgtc
     3601 ccggcgcgac cgaagcgcat gcgctggaac ggccgctcga tcgtccagcc gccatttgtg
     3661 aggccttgca agaaaatctc ttgcgaggcg acggcgtcga tcaggagcgg gtgcttctcg
     3721 tcggcagcga tgtcatgcac catcgcaagc gcatgtgtcg gcgtgccggc aaacagcgga
     3781 ccgatatggc gcgccgtgcg accgtcgcgc acgagcgcca tcgcgtcgcc atgcgacagc
     3841 agccgcgaag ttgcgcgttc gctcagctcc cgcagcagat cgcggcggtc gaagcccatc
     3901 gcgcggatgt ccagtttgag caaatcgtcg agcttgccgg gtgcgagctc gacatcggct
     3961 ttcaccgtcg atgctcgttc aaaccgcagc cgccgcaact ggatggtggg cgagaagccg
     4021 agtgggccgt agacggcggc acccgccggc gtcgcgtcga gccaggcggt gaccccgagc
     4081 ctcgtagccg caccgatgca ggcatcgacc agccttgtcg cgaatccgcg acggcgccag
     4141 ttggcggtga cgagcaccat gctgatccat gcatcgccat cgctgtaagg caacaacgcc
     4201 gcgcttgcga tgacacgacc atcgcggtcg cggacgccgt acacaatgcc cttggtgagg
     4261 aagaatcgcc aatccgcctc attctggttc cattgcgctt cgctcgacag caggacgccg
     4321 cttgccgtat cggcctcgcc gagttggacg atggcaagtt gctcagtagc gtccatcgcg
     4381 gacctcatat ttggtgggct tgccgagaga cggcatggcg cgcgcaaccc aatccgcagt
     4441 ccatttgatc agttgctcgg tgtcaacgat cggcaggccg aacagcttcg tcgccagcga
     4501 ggtgttggtg agccaggcgg tcggctcttc cctgcctgtt atgaccggcc tacacccgag
     4561 caaatcgccg aaccttgccg ccagatcgcg cacggcgagg atttcgtggc ctgagacatt
     4621 gatcggcgag gtcggcgtct cgcagtgcgc aaggcttcgt agcgcttgcg atgatgcatc
     4681 gccttgccag atgaaattga cgtgaccgag ggtgacgtcg atcggcgtgc cgtcgcgcac
     4741 cttggtcgcg atgtcgtgca gcacgccata gcgcatgtcg atggcatagt tcaggcgaaa
     4801 cagtctgcca ggcgtcctga acgcgcggga aaaatattcg aacatgcgct cgcggccgac
     4861 gcaggattgc gcgtactcgc cgggcgggtt tggtggcagg ctctcgtcgg cacctgcgcc
     4921 gtcaatcggt acaaagggat aaacgcagcc ggtggagaag gcgacgatcc gcgaactgcg
     4981 aaaggcttgc gcgaccagcg cggggacatg ggcattcatc gcccaggtca gcgccagatt
     5041 gccttcggcg ccgaacttgc gcccggccat gaagatgatg ttggtgacct gcggcaagcg
     5101 gcggatcgca gcttcgtcca aaagatcgca gtgaatggtt tcgatgccgc gcgccttcag
     5161 ccatggctcg atgcctgctt cgctgaagcg cgcgacgccg atgatccgcc gctccggcag
     5221 cgcagccttt gccagccccg cgagcgtcgg ccccatcttg ccgccgacac cgagaatcat
     5281 gatgtcgcca tcgactctgc cgagatcgtc gatcagcgcc tggctcggcc ggcacaacag
     5341 ctcgtcaagc gccgcaatat cgggaatcgt ctccggcaga ttgtcgcgca atagcagcat
     5401 gtttcttcct gtgattacta ttgcagccgg atgtcgcgca ccacgaacag ccgttcgagg
     5461 cccagcacca ggccgtaaag tccgacgccg agcagcgtga gcagcaccac cgccatcacc
     5521 atcgccggcg tgtcgagcga ggactgcacc tggatcatca ggtagccgag cccgcggtcc
     5581 gacgcgatga actcgccgac gatggcgccg gcgacggcga ggatggcgcc gaccttcatc
     5641 ccggaaaata cgtaaggcag cgagcccggc agttgaatct tgcgaaacag gatccaacgc
     5701 gagccgtgca gcgatttgac gagatcgagc aggtccggtt ccacctcgct caggccgcgc
     5761 gccgtggtga gcaggatcgg aaagaaacag atgctgaacg cgatcaggat attggcgatg
     5821 atgccgtagg agaaccagac gatgaccagc ggtcccatcg cgaccttggg aatcatgttc
     5881 agcgtgacga acagcggcaa aagcaggaga gcgaccagcc gcgaccaggt gaagatcagt
     5941 gcgagcgcca cgccgacgac ggcgccgagc aggaagccgc cgaggatttc gatcgccgtg
     6001 acggcaaggt tggcggccca ggcatagttc ggatgcgaca aggtcgcgag agccgcaagc
     6061 ggcgaaggca ggatgaattt cggcacgtga aaagcatcga ccaggacctg ccaaagcacg
     6121 atcacggtca gatgtacgat caggatgatc gcaaaggatc tcgccgatcg ctcgccgcca
     6181 ccgaacaccg tccgctcctt ccgccttcat caaacgggtt gcgccgaaat ctatccgggc
     6241 gcctgcacag tttcaaccac ctggttgatt agatcatcac ggtcggagcg cctgcagcac
     6301 gaggtcgacc acatgcttgc gacgggcgct tcttgcggcc gcgctggcca gatccttgcc
     6361 gaagatggcc gatagtgtcg gcgtgttcga aaagaagaag tagctcaggc cggcgatcga
     6421 aatatagagg tgcacggggt tgatgccttt gcgaaacacg ccggcccgta ccccttcgga
     6481 aagaatcttc gaaaccatgt tgacgagcgg cgaatgcatg gcttccagcc gccgcgaggt
     6541 gcgcacatgc ctggcgccgc cgcggttctc gtcgttcaac agcaggatga aatcgggatg
     6601 cgcggcgagg tggtcgaacg aactttcgat cagcttgcga attgcctggt ggggcggcag
     6661 gcctgtcaga ttgagcttgc gctcctgcgc gcggatctcc tcgtacaccc attcgaggac
     6721 cgcaagataa agcgcgtcct tgtcgccgaa atagtggtag acgagttgct tgttgacgcc
     6781 cgcgcgcgtg gcgatctcgt cgacgcgggc accggcaagc ccgctattgg cgaactcgcg
     6841 acgcgccgcg gcgagcagct tgcgtcgcgt ggcttcggga ttgcgccgct tcggcttctt
     6901 gccgttggct cgtcgatcgg gcatttccaa ccagacagtt gacaactcgc gcggtcacct
     6961 gttgcattgg taacgtcgcg ggccggggac gacaagtccg gttttgcaat ttcgaggagc
     7021 gtcgtcgtga agtacggttg ccttgcaagg ccgaagcttg ccggtctggt gctcgcgctg
     7081 gtgatgtcgt gcgctccttg ccgggccggc gaggcggtca atctgttgct gaactggacc
     7141 ccgactgcgg accattcccc gttctactac gcgaaagcgc aggggtggta cgagaaggcg
     7201 ggcatcgatc tcaccatcga aaccggcaag ggctcgggcg tctcctcgct gaaagtcggc
     7261 tccggcggct cgccctttgg catcgccgac ctcgcaacca tgctggtggc gcgcagcaag
     7321 ggcgccgacg ccgtggctgt catgagcatc tacgccaata ccgggcagac attttattgg
     7381 ttgaaaagct atggcgtgaa cggacctaaa gattttcccg gtcacaagat cggcaatccg
     7441 cccggcgatg cttcgcgcgt gatgtggccg gcctttgcga aagcggccgg cattgcgccc
     7501 gactcggtga atttcgtcaa tgtcggcccg acggcgaaga ttgccgcgct caagagccac
     7561 accgtcgata tcatcagcga cttctacaac gagcacgatc tcaaggtgat cgaattcgat
     7621 ggcgacctcg gctatgtcaa ctggaaggac atcggcctca atccttacgg caattcgctg
     7681 atcgtcaacg gcgcgttcct ggagaagaat ccgaagctgg tcgaggattt cgtcaaggtc
     7741 acgcagaagg cctatgccgc ctgcgttgcc gacgtcgccc cctgtctgaa ggcgctgctc
     7801 gatcaggtct cggggttgga caaggagaac cagcagcgcc agtgggagcg catcaaggtg
     7861 ctgatgacgg acgagtttac gaccgcgaag gcgctggggt ggatcgacgg cgagcgcatg
     7921 aagaaggact acgagctcgt gcagacctat ctcggcatgg aaaagccgtt cgacgtgcag
     7981 acggcgttca ccacccggat gcttgatccg tcgatcaaga tggatgcaag caaggtgaag
     8041 aagtaaaact cttcttgtca cacgcgggcg tgacccgcgc gtccgtcaaa acaggaatcg
     8101 attgggaagc cgggtcgggc ctggccacga cgagacggga gctactgcag cgccatcccc
     8161 gaggtcttca ccaccttgtt gagcttctcg tagtcggcct tcatcagcac ggtcagatcg
     8221 ctcaacggca tcggcttgcc cggaatatcg gagatcgcga gctgacgctg cacgtcggga
     8281 ttctccagcg ccttgtcgat cgcatcgtgg atcttttcga ccagcgcgtc gggagtggct
     8341 gacggcacat aaatgccgta ccagggca
//
LOCUS       DATLJT010000506         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_718390,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000506 DATLJT010000000
VERSION     DATLJT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718390"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..2050)
                     /locus_tag="VM910_11715"
     CDS             complement(416..2050)
                     /locus_tag="VM910_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63233.1"
                     /translation="MTDEHVTAPTGLPACLRRLHWLTLLAGVAAFLATPGAPAAEASP
                     TRGGILAFAVDAEPPNYDCHANFSFVFIHPAIPHYSTLLKFDTANYPQIVGDLAQSWD
                     VSADRRTYTFKLRDNVLFHDGSRLTSEDVKASYDRIIHPPQGVLSVRQADYAAITDIE
                     TPDPMTVVFHLQWPDAAMLANFASPWNCIYNAAKLKEDPLFPKTHVLGTGPFVFVEHV
                     KGNHWTGKRWDKYFVPGKPYLDGYRADFIAGPAAVKAMESGRIMAQFRSFTPTERDEM
                     VKAAGDRLEVREGPWITYLAIVFNATRPPFDDARVRRALSLAIDRWHGAETLADNTFL
                     KYVGGLMRPGTATSMPETELVTLPGFSHDIAASRAEARRLLAEAGVPNLAVTLTNRKD
                     VPVPYGAAGDVVVGAWREIGVQATQELLSTKDWQTALETGHFAAATDLAADYFDDPTI
                     QLARYVSRDLSPTNHSGSTDRFLDALYIGQALSTDPLQRAKIVRAFERHALNDAYTVP
                     LLWWNRIVVTGEKFKGWNISPSHYIGQDLADVWLEE"
     gene            complement(2089..>2592)
                     /locus_tag="VM910_11720"
     CDS             complement(2089..>2592)
                     /locus_tag="VM910_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI63234.1"
                     /translation="LSRDDGLHFLAFPFNPAVPAAYVPTRLTATDYPGLIPQDRPVDT
                     VAVGAVLVVANLQMAPERDRNVANFVDAFFTGFHSLLEPGHHPKWQEVNLAAELTGWR
                     RYPPAEQWLQRNTQVAKAPNPDDLVSMFARFVDERRQSIGGAPMTQQEKTDLFQQYHH
                     WEAGQPQ"
BASE COUNT          476 a          765 c          857 g          494 t
ORIGIN      
        1 ggtatctgct gcgctgccaa caggtcaagt gtgtccgcaa ccaggccggc aaaccgtgca
       61 gacaccccaa tgcgacacca aaaccgccca tcccgtacca tcgctgtctg cggaaatcgc
      121 tatgcccctc atccacccgc cggccccctg tccagacgtt ccctaatagc gcggcgagtg
      181 gggtttacga tcaccgcagc gcaacgcgga ggacagtaac ggtaaggtga tcctcagggt
      241 ttgcatggcg ctgtcgttgc gagctctttg atccgtcctt tatcggccgc agccaacgat
      301 atcggggtga gccgcttgca gctaagccgc ttgctcccaa gatggcaacc attcggttct
      361 ctaactccga tggccccgat gccggcaggg tgttttgttc gggatcggcc gcgcgtcact
      421 cctcgagcca cacgtcggcc aggtcttggc caatgtaatg gctcggcgat atgttccagc
      481 ccttgaattt ttccccagtg acgacgatgc ggttccacca caggagaggc accgtatagg
      541 cgtcgttcag ggcgtgccgc tcgaaagcgc ggacgatctt ggcgcgttgg agcggatcgg
      601 tgctgagcgc ctgcccgata tagagcgcgt cgagaaagcg gtcggtcgag cccgagtggt
      661 tggtcggcga cagatcgcgc gaaacgtatc gcgcgagctg tattgtcgga tcgtcgaaat
      721 agtccgcagc cagatctgtc gccgcggcaa agtgcccggt ttcgagcgcc gtctgccaat
      781 ccttggtgct cagcagctcc tgggtagcct gcacgccgat ctcgcgccag gcgcccacga
      841 cgacgtcgcc ggcagcgccg tagggaaccg gaacgtcttt ccgattcgtc aatgtgacgg
      901 caaggttggg cactcctgct tcggccagaa gccgccgcgc ttctgcgcgt gaggcggcga
      961 tatcgtgtga gaagccggga agcgtcacga gttcggtctc gggcatgctg gtcgcagtgc
     1021 ccggccgcat cagaccgccg acatatttga ggaaggtgtt gtcggcgagt gtctccgcgc
     1081 cgtgccaccg gtcgatcgcg agggaaagcg cgcgccgcac ccgggcatcg tcaaacggcg
     1141 ggcgggtggc gttgaacacg atagccaggt aggtgatcca ggggccttcg cgtacttcta
     1201 gacggtcccc cgccgcctta accatctcgt cgcgctcggt cggggtgaag ctgcggaatt
     1261 gggccatgat gcggccgctc tccattgctt tcaccgctgc aggaccggcg atgaaatccg
     1321 cccggtagcc atcgagataa ggcttacccg ggacgaagta cttgtcccag cgcttgcccg
     1381 tccagtggtt cccttttacg tgctcgacaa agacgaacgg cccggtcccg agcacgtgcg
     1441 tcttcgggaa cagcggatcc tctttgagct tggcggcgtt gtagatgcag ttccagggcg
     1501 aagcgaagtt tgccagcatc gccgcgtcgg gccattgcag atgaaacacg accgtcatcg
     1561 gatctggcgt ctcgatgtcg gtgatcgcgg cgtagtcggc ctgtcgcacc gacaggaccc
     1621 cttgcggcgg gtggatgatg cggtcgtaac tcgctttgac gtcttctgaa gtgaggcgtg
     1681 acccgtcgtg aaagaggacg ttgtcacgca gcttgaaggt gtaggtacgc ctatctgcgg
     1741 agacatccca ggattgggct aaatcgccca cgatttgcgg atagttggcc gtgtcgaatt
     1801 tgagcaaggt ggagtagtgc gggatcgccg gatggatgaa gacgaaggag aaattggcat
     1861 ggcagtcgta gttgggcggc tcggcatcga ccgcaaacgc caggatgccg cctctcgtcg
     1921 gggaggcttc agctgccggg gcgcccgggg tggcgagaaa agcggcgacg ccggcgagga
     1981 gtgtgagcca atggagcctg cgcaggcagg caggcaggcc agtcggcgcg gtgacgtgtt
     2041 catccgtcat tgaaatttcc tttgaaaggg gatggcaggg gtcgccgact actggggctg
     2101 gccagcttcc cagtggtgat actgctggaa gaggtcggtt ttctcctgct gcgtcatcgg
     2161 cgcgccgccg atcgactgtc ggcgctcgtc cacgaagcgc gcgaacatcg acaccaaatc
     2221 gtcggggttg ggggccttgg ccacctgcgt gttgcgctgc agccactgct cggcaggcgg
     2281 ataacgccgc caaccggtaa gctccgcagc gagattgacc tcctgccatt tcgggtggtg
     2341 gccaggctcc agcaacgagt ggaatccagt gaagaacgcg tcaacgaaat ttgcgacatt
     2401 gcggtcacgt tcgggggcca tttggagatt ggcgacgacc agcaccgcgc ccacggcaac
     2461 ggtatcgacc ggccgatcct gcggtatgag acccggataa tccgtcgccg ttagccgagt
     2521 cggcacataa gccgctggga ccgccgggtt gaacggaaag gcgaggaagt ggagcccatc
     2581 gtcccggctg ag
//
LOCUS       DATLJT010000507         5394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4394936,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000507 DATLJT010000000
VERSION     DATLJT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4394936"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VM910_11725"
     CDS             complement(<1..207)
                     /locus_tag="VM910_11725"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVI63235.1"
                     /translation="MSNDSTRQHLIGTWKLITAVREEISSGTKTEFLGPSPVGYINYG
                     ADGRMLVLTVASGRRKPAGPKATSA"
     gene            complement(313..1752)
                     /locus_tag="VM910_11730"
     CDS             complement(313..1752)
                     /locus_tag="VM910_11730"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HVI63236.1"
                     /translation="MDEGSITMATGVVVVYFSRFGKPLQTHHKVVLDLGAKAIAKLKG
                     YEFGGHYDAACDYSGPLFFVPDDTLLLDEAACLGIRSSNDLYGGVVPHLFAKTKAITH
                     GLVDRDAERPLGWCTAFAERVREIVLPGYSVFSNRDARVAAGCMLVRGPIRVKKPLSA
                     SGKDQTVVTTLNELDAVLEKASADEMATYGLVLEENLRQVRTLSVGEVAVGGLRISYH
                     GTQRTVADNEGRPVYGGSELVCVRGGWEVLDTLPMSPEVRAAVAAARRYDEATEEFEG
                     FTASRRNYDVARGTGADGRPRSGVLEPSWRVGGASSAELAAFAAFARDPSLEIVGASH
                     VEEFGTGRRAPADAIVEFQGDDPEWGPLLRYTIVEPQDQQSRQKNPGRCGEAWRGLPP
                     PAISLGTDSRVIRYRSRAGASHQSQRGKAPIGYSGPDHSPVADLSKYECPQGEDDYRH
                     RMVVNAIALVFVSLLSLAGFWLVNAIAHS"
     gene            complement(2011..2586)
                     /locus_tag="VM910_11735"
     CDS             complement(2011..2586)
                     /locus_tag="VM910_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63237.1"
                     /translation="MFVGYGSGALAVIDPATNGKIADIPLQAHPESFQLARSDRRIFV
                     NVPKAREIAVVDRFASKQTASWPIDNGSNFPMALDESSGRLFVAFRNPPQLNVFSLRD
                     GSTIAAVGACGDTDDLFFDAKRQRVYLSCGDGYVDVFEAQEDAYGRSAHIPTISGART
                     ALFVPELDRFFVAARANPGEPAALWVFRPIP"
     gene            complement(2550..3017)
                     /locus_tag="VM910_11740"
     CDS             complement(2550..3017)
                     /locus_tag="VM910_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63238.1"
                     /translation="MMMRYSCVVYLVAIATVSLAPDRAFSQTPSSSSLQLETKIPLGD
                     VKGRIDHMAIDPARRRLFVAELGNDTVGVVDLNEQKVQHVITGLKEPQGLGYVPSSDT
                     LFVTNAGDGSVLLFRGAEYAAAGRIDLGDDADNIAWIARPTECSLVTGAAHWR"
     gene            complement(3014..3370)
                     /locus_tag="VM910_11745"
     CDS             complement(3014..3370)
                     /locus_tag="VM910_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63239.1"
                     /translation="MPGSSPDSFNEYIGDVTAATWTPKKGAFADLTHIAPSAQYHAPL
                     SMTKVRIPTIIQKASAQGAILSVREKIRGGEPVFEMMVIQDKGNPTDILRPRLRRADS
                     RLRKNSWNPMDRRDSQ"
     gene            complement(3360..4427)
                     /locus_tag="VM910_11750"
     CDS             complement(3360..4427)
                     /locus_tag="VM910_11750"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVI63240.1"
                     /translation="MRPHLCFAGIALLVVVAPAIAAETETERLKQAQGLFQPLPRDMA
                     TPEAPITPQRVDLGRLLFFDPRLTVDADVSCSSCHQPARHGTDGLPKSIGVRQRPHPR
                     HAPTILNAGLNFIIHWRGDRDSLEDQVIKALASPITNGQPDEKAIIGRLEQIPSYAPL
                     FKAAFPDDPHPMTSENIAKAIGAYERTLVTPSPFDRYLAGDVNALSPKARGGLDTFIN
                     TGCAACHNGVLVGGGMYRKFGVVEPYWKATGSQPIDNGRADVTKNPDDLYVFRVASLR
                     NVAMTAPYFHDGSVATLPEAVRVMARVQLGVTLGDAEISDIVAFLQSLTGEVPANFAT
                     VPTLPPGSVTPPGEGSKESAR"
     gene            complement(4509..4709)
                     /locus_tag="VM910_11755"
     CDS             complement(4509..4709)
                     /locus_tag="VM910_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63241.1"
                     /translation="MNRLIACMIGGAVHQPFGGNETTSHIVSTDLSQIYVAASWVGTA
                     ELAIVAAGLGLWLRQRKVSQRI"
BASE COUNT         1045 a         1648 c         1619 g         1082 t
ORIGIN      
        1 ggcggaggtc gccttcggcc ccgcgggctt gcggcggccg ctcgccacgg tgagcaccag
       61 catgcgcccg tcagcgccgt agttgatgta gccgactgga ctgggcccca gaaactcggt
      121 cttggtgccg gaggaaattt cctcgcgcac cgccgtgatg agcttccaag tgccgatgag
      181 atgttggcgg gtggaatcat tggacatggc gcaagcttta acgcgtcgcc gtcacggagc
      241 aaagtgcgcg tcgtggtaag ggggaaaagc acgatgcagc ggaaggtcgc ctcgtggatg
      301 catgcttgcg cttcagctat gcgcgatcgc attgaccagc cagaagcccg ccaggctcag
      361 aaggctgaca aagaccaggg caatggcgtt tacgaccatg cgatgccggt aatcatcctc
      421 gccttgcggg cattcatatt tcgagaggtc ggcaaccggt gagtgatctg gcccggaata
      481 accgattgga gccttgcccc gctgactttg gtgcgaagcg cccgctcgtg accggtatcg
      541 tatgacacgg ctgtcagtgc ccaatgagat ggcgggcggc ggcaagccgc gccacgcctc
      601 gccgcatcgg ccggggtttt tttggcgcga ctgctgatct tgtggctcca cgatggtgta
      661 tcggagcagg ggcccccact cgggatcgtc gccctgaaac tcgacgatcg catctgcggg
      721 agcccggcgg cctgtgccga attcctcgac gtgggatgcg ccaacgatct ctaacgacgg
      781 atcccgcgcg aacgctgcga acgccgcgag ttcggcgctg ctcgcgcccc cgacacgcca
      841 ggaaggctcg agcacgcctg atcgtggccg accgtcagcg ccggttcctc gagcgacatc
      901 gtagttccgg cgcgacgccg tgaagccctc gaactcctcc gtcgcctcgt cgtatcgtct
      961 tgccgcggcc acggcggcgc gtacctcggg cgacatcggg agtgtatcga gaacctccca
     1021 accgccacgc acgcacacga gctcggatcc gccgtaaacc ggccgcccct cgttgtccgc
     1081 cacggtcctt tgcgttccgt ggtagctgat ccttaggcca ccgaccgcga cctccccgac
     1141 gctcaacgtc ctgacttggc gcaagttctc ttcgagcacc aatccatacg tggccatctc
     1201 gtcggcgctg gccttctcca gcacggcatc gagctcattc aaagttgtaa ccaccgtctg
     1261 atcttttccc gacgcactga gcggtttctt cacgcgaatc ggtccgcgca cgagcatgca
     1321 tccggccgcc acccgagcat cgcggttgct gaacacgctg tagcccggca acacgatctc
     1381 gcgtacccgc tcggcgaaag ccgtgcacca gcccagcggt cgctccgcgt cacgatcgac
     1441 caggccatgg gtgatggctt tcgttttcgc gaaaagatgg ggcacaacgc cgccgtagag
     1501 atcattcgaa gaacgaatgc cgagacatgc tgcttcgtcc agcagcaagg tgtcatccgg
     1561 aacgaagaag agcgggccgg agtagtcaca tgccgcatcg taatgcccgc caaactcata
     1621 gcccttgagt ttcgcgatag ccttggcgcc caagtcgagc acgactttgt gatgcgtctg
     1681 cagcggcttg ccgaaccgcg agaagtagac cacgacgacg cctgttgcca tggtaatcga
     1741 cccttcatcc atgcgcccaa tccgcgatgg ctgctgagcg gcactcccga gagtgcgttc
     1801 ttccttcagc gcgccgatcc ttctcgtctg ggcagacgag catgatcgcc ctgatgctgc
     1861 agagggccgt ttaaggcagg tttatgccga gttgggcggg atgatcggcg gcagtcacaa
     1921 gtccgagagg cctatgttat caaccaggtc tgccggtatg gcgacgcaag tcgcaatggt
     1981 ggacatgttg gaactgagat tgcgctcaac ttacggtatt gggcgaaata cccagagcgc
     2041 tgccggttcg ccgggattgg cgcgagcggc gacgaaaaac cggtcgagtt cgggaacaaa
     2101 cagcgcagtt cgtgcgccgg atatcgtcgg gatgtgggcc gagcggccgt aggcgtcctc
     2161 ttgcgcctcg aacacatcca cgtatccgtc gccgcagctg aggtacacgc gctggcgctt
     2221 cgcatcgaag aagagatcgt cggtatcacc acacgcaccc acagcagcaa tcgtgctgcc
     2281 gtcccgcagg gagaacacgt ttagctgcgg gggattccga aacgccacaa aaagtcggcc
     2341 ggaactttcg tccagagcca ttggaaaatt gctgccgttg tcgatcggcc agctcgctgt
     2401 ctgtttgctc gcaaaacggt cgacgaccgc gatctcgcgc gccttcggca cgttcacgaa
     2461 aatgcgacgg tcagagcggg caagctgaaa actttcggga tgagcctgca atggaatatc
     2521 ggcgattttg ccgttcgtcg cgggatcgat caccgccagt gcgccgctcc cgtaaccaac
     2581 gaacactcgg ttggacgcgc tatccacgcg atgttgtcgg catcatcgcc gagatcgatt
     2641 ctgcctgctg ctgcgtactc cgcgcctcgg aacagcagca ccgatccgtc acccgcattg
     2701 gtgacaaaaa gcgtatcgct cgatgggaca tagccgagac cttgcggctc cttcaatccg
     2761 gtaatgacgt gctgtacctt ttgctcattc aaatcgacga ccccgacggt atcattacca
     2821 agctcggcta cgaacagacg ccgtcgggcc ggatcgatcg ccatgtgatc gatccggccc
     2881 ttaacgtcgc ccaacggaat cttagtttcg agctgcagcg acgaggagct cggcgtctgt
     2941 gagaatgccc gatcgggtgc aagcgacacc gtcgcaattg ccacgaggta caccacgcaa
     3001 gagtaacgca tcatcattgg ctatctcttc tgtccattgg gttccaactg tttttcctca
     3061 gccggctatc ggctcgccgg agacgaggtc gtagaatgtc ggttggattg cccttgtctt
     3121 ggatcaccat catttcgaaa acgggctcgc caccgcgaat tttctccctg acggacaaga
     3181 tcgcgccctg cgccgaagcc ttctggatga tcgtcggaat cctgaccttg gtcatcgaca
     3241 gcggcgcgtg atactgcgcc gatggagcga tgtgcgtcag gtcggcaaac gcccccttct
     3301 tcggggtcca ggtcgccgcc gttacgtcgc ctatgtactc gttgaaggaa tcagggctgc
     3361 taccgggcac tttctttgct gccctcgccc gggggcgtga ccgatccggg cggaagggtc
     3421 gggacggtgg cgaagtttgc cggcacttcg cccgtcaggc tttgcagaaa cgccactatg
     3481 tcgctgattt cggcgtcgcc cagcgtcacc ccgagctgca cccgcgccat gacgcggaca
     3541 gcctccggaa gcgtcgccac ggacccatcg tgaaaatagg gcgcggtcat cgcaacattc
     3601 cgcaggctgg caacgcggaa aacataaagg tcgtcggggt tcttggtgac atcggcgcgt
     3661 ccgttgtcga ttggttggct gcccgttgcc ttccaataag gctcaacgac gccaaacttt
     3721 cggtacattc cgccgccaac gaggacaccg ttgtggcacg ctgcgcagcc ggtattgatg
     3781 aaggtatcga ggccgccgcg agctttgggc gacaatgcat tgacgtcccc ggcgaggtag
     3841 cgatcaaatg gcgatggcgt caccaaggtc cgctcatagg ctccgatggc cttggcaatg
     3901 ttctcgctcg tcatggggtg aggatcgtca gggaaggccg ctttgaacag cggggcgtaa
     3961 ctcggaattt gttcgagtcg gccgatgatt gccttctcgt ccggttgccc gttcgtgatc
     4021 ggcgaggcga gggcctttat cacctgatcc tcaaggctgt cccggtcgcc gcgccagtga
     4081 atgatgaaat tgagcccggc attgaggatt gtcggggcat ggcgcggatg cggtcgctgc
     4141 ctcacaccga tcgacttggg cagcccgtcc gttccgtggc gtgctggttg atggcaactg
     4201 gaacaactga catcggcgtc gaccgtcagt cggggatcga agaagagcag ccggccgaga
     4261 tcgactcgtt gcggcgtaat cggcgcctcc ggcgtcgcca tgtcccgcgg tagcggctga
     4321 aagagtccct gagcctgttt gagccgttcg gtttcggtct ccgctgcgat tgctggcgct
     4381 acgacgacca gcagtgcgat gccggcgaag cagaggtgtg gcctcatcac ctatctccct
     4441 tggtcatcaa cccgcttacc cctcccggct tcgaagcttt cgcgaaatct tgatctgctg
     4501 tgcggagatc agatgcgctg ggagacctta cgctgccgaa gccagaggcc gagaccggcc
     4561 gcgacgatgg caagttccgc cgtgcctacc caagaggcgg caacatagat ctgcgatagg
     4621 tctgtcgaga cgatatgact ggtcgtctca tttcccccaa agggttggtg taccgcaccg
     4681 ccaatcatac atgcgataag tctattcatg gctctctcct tcgcggtaga tacatcgagg
     4741 agtagagcga agcggtctac atttaaggca aaattaaacg tcggcctgcg ttaatcacag
     4801 atcggtgagg tcgcgacttt cgtatcgagc gctgtcgtag aacctggcac tttgatccct
     4861 tctgcacgaa ccgcggctcc gtcacgcgca agacaactgg cgacattcgc ctggccgtct
     4921 ctctgattac accacgtagc ctctctgatt acaccacgta gcgcgactta ggagacgcgc
     4981 aacatgcgta ccgaggtcga cctgcccaac cccgaggaac ggctttaatc caggcatgta
     5041 tgcggagctc tcgctggaaa tgaacaggcg tcttgatgtg cttaccgtgc cggccgctgc
     5101 tgtaggcttg gatggtgacg gtaacttcgt ttacaccgtc aatgacgatc gcatgacaca
     5161 cctgcggctt aaaactgtct taaactgcgc atgtatctga agcaacaggg gtaagtcggt
     5221 ttcccggaga ggcaagtaca tgcgtcttct ggtcgtcgaa gatgagccgc gaatttcgga
     5281 aatcatcaaa gctgcgctgg agcgcgccga gtttgccgtg gacaccgtgc ggctgcgcgc
     5341 cgatgcgcgg gaagcgctgg caaacaccgc ctacgatgcc gcaatcctcg acct
//
LOCUS       DATLJT010000508         2027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3246832,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000508 DATLJT010000000
VERSION     DATLJT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246832"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VM910_11760"
     CDS             <1..297
                     /locus_tag="VM910_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI63242.1"
                     /translation="PVDNAGKRLMDFAMEADVHDERLWMPIARATGALGNTSCLVGTP
                     EQVADAILEYYRLGVDSFLIRGFDPFNDTIEFGRELIPRIKAGAIEIDGLKAAG"
     gene            412..1395
                     /locus_tag="VM910_11765"
     CDS             412..1395
                     /locus_tag="VM910_11765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63243.1"
                     /translation="MMHQALKVVAVFVLGLCGTLGATVCGNSQTWPPNPVRFIVPFPP
                     GGTTDQIARRVQPLLQTELKTTIIIENRGGAAGAIGTQAAATAAPDGATFLLVFDSHV
                     VNPSILPNLPFDARKDLAPIMLIGTSPMVITAHPASEYKRLDDVIAAARKKEGAVGYG
                     TIGAGSLAHLTMSQIGNELKVVMTNIPYKGGGPLTSDALAGHVQVAIGSVALMSPHIK
                     ASALRPLAVTSANRFPQLPDTPTLRESGIPVDAESWWGILAPAKTPSEIIARMHAAMS
                     KALQEPAVQRSLSEQGVILRVSTPEAFGTFIDSEITRWAKVVKDNKIVAGD"
     gene            complement(1820..>2027)
                     /locus_tag="VM910_11770"
     CDS             complement(1820..>2027)
                     /locus_tag="VM910_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63244.1"
                     /translation="CARRERPRRLRADEQCDEVAPTHPDARPINTPMLRIRSPCCARA
                     ASGRTAAPPRSVAKNFRRSMWLAM"
BASE COUNT          386 a          640 c          640 g          361 t
ORIGIN      
        1 cccgtcgaca atgcgggcaa gcgcctgatg gactttgcca tggaggccga cgtccatgac
       61 gagcgcctgt ggatgccgat cgcacgcgcg accggtgccc ttggcaacac gtcctgtctc
      121 gtcggaacgc ccgagcaagt cgccgatgcg atcctcgaat actaccgact gggcgtcgat
      181 tcgtttctca ttcgcggctt cgacccgttc aacgacacga tcgagttcgg ccgcgagctc
      241 attccgcgga tcaaagcggg cgcgatcgag atcgacggtt tgaaagcggc ggggtaggtc
      301 catgtgtagc ccgcatgggc gcagcgacat gcggggtcat aggcaggacc cggatgtcgc
      361 tgcgctcatc cgggctacgc gctcatccag ctgcgcaatc ttggagaaga catgatgcac
      421 caagcgctga aagttgttgc ggttttcgtc cttgggctat gcggcacgtt gggtgctaca
      481 gtttgcggca acagccaaac ctggccgccg aatcccgtcc gcttcatcgt gccatttccg
      541 cccggcggga ccaccgatca gattgcgcgg cgcgttcagc cgctgctcca gactgagctg
      601 aagaccacca tcatcatcga gaatcgcggt ggcgcagcgg gcgcgatcgg cacccaggcg
      661 gcagccaccg ctgcgcccga cggcgcgaca ttcctcctgg tgttcgacag ccatgtggtg
      721 aacccaagca tccttcccaa tctgccattc gatgcccgca aggatctcgc cccgatcatg
      781 ttgatcggca cctctcccat ggtcatcacc gcccatcccg cgagcgaata caaacggctt
      841 gacgatgtga tcgcggcagc gaggaagaag gaaggtgcag tcggttacgg caccatcggc
      901 gcgggcagcc tcgcccatct caccatgagc cagatcggca acgagctcaa agttgtgatg
      961 accaacatcc cgtataaagg cggcgggccg ctgacgagcg acgcgctcgc cggtcatgtt
     1021 caggtggcga tcggatcggt cgctctgatg tcgccgcata tcaaggccag cgcgttgcgg
     1081 ccgctggctg tgacctctgc caaccgcttt ccgcaactgc cggatactcc gacgttgaga
     1141 gaatcgggca tccccgtcga tgccgagtcc tggtggggca ttctggcgcc ggccaagacg
     1201 cccagcgaga tcatcgcgcg catgcacgcc gccatgagca aggccttgca ggagccggcg
     1261 gtgcagcgga gcttgtccga gcagggcgtg atccttcgcg tgtcgacccc cgaggcattc
     1321 ggcaccttca tcgacagcga gatcacgcgc tgggccaagg tggtcaagga caacaagatc
     1381 gtggcgggcg attagcaccg attcgatgat gttgactcgt aggggcgggt tttaaacccg
     1441 ccccgattca gcaagcaccg gtttcgaagt tgtggcgcat gatggacaaa atcatacgcc
     1501 tcgcgcggag agggagggcg tgccaaggcg ctcggactcg atgtatcgcc caccctgctc
     1561 gcccgtgccg atgaggtgat cgaataatgg cctagcttgc tgcaattgct cacaaccgct
     1621 attggcacga agcggacgtg ccggcacgca gcaacgatgt ctgctcttgg ggcaaaaccg
     1681 gacgacacat gctcgttgag agccttcgag cggtcttcgg tagcatccat aggacctgcc
     1741 aatcgcggcc ttagtcacag accgcgcgat ggaagcgtgg tatcatccct ccattgcatg
     1801 aatgacccca accggagggt cacatggcaa gccacatcga acgacgaaaa ttcttggcca
     1861 cgcttctcgg cggcgcagcg gtgcggccgc tcgcggcgcg cgcgcagcag ggcgagcgga
     1921 tgcggagcat cggcgtgttg atgggccgcg cgtccggatg cgtcggcgcg acttcatcac
     1981 actgctcgtc ggcacggagg cggcgtggcc gctcgcggcg cgcgcac
//
LOCUS       DATLJT010000509        15744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4395021,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000509 DATLJT010000000
VERSION     DATLJT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395021"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..1059)
                     /locus_tag="VM910_11775"
     CDS             complement(622..1059)
                     /locus_tag="VM910_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63245.1"
                     /translation="MIIIGIVLSFIGLGYLCWLVFALAVNALPLFAGVTAGLAAYHSN
                     SGTIAAVAIAAIVSVIVLFAGQLSFRTSRSTPVRAAIALLFAVPAAIAGYHAAHGLAD
                     AVVPIEGWRETVGITGAIMVATIAWARITLSAPNDAGRALPLA"
     gene            1361..1810
                     /locus_tag="VM910_11780"
     CDS             1361..1810
                     /locus_tag="VM910_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HVI63246.1"
                     /translation="MTSPTPQPVIERVFRKLGGYRALGSDVASEADLARIVDRGIRLV
                     VLGYVQKAGFSKQEIERLIIPARTWRHRKVKGEPLSTEESDRVVRLTRIQALAEDVFG
                     DADKANRWLREELGILAGKSPLETARTEAGARLVEQLLAKLDWGAAA"
     gene            1811..2278
                     /locus_tag="VM910_11785"
     CDS             1811..2278
                     /locus_tag="VM910_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HVI63247.1"
                     /translation="MRLWRLSSERRARDFDGGYGLWNDGRWNSRGRPVTYCSTVVSLT
                     ALEKRVHVSDPALLPPQALVAYEVPDGVSRRTIAIDDLPLDWTLRETHTQTIGDQWLD
                     SQTEVLLFVPSVIVPIANVPDRNVLINHRAAEAAAIHIAQVIPFAFDPRLFAP"
     gene            complement(2409..3341)
                     /locus_tag="VM910_11790"
     CDS             complement(2409..3341)
                     /locus_tag="VM910_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HVI63248.1"
                     /translation="MTTDRDELEFSSLHASSPTDRILTELQLHGHRPFQDEPDPRPLP
                     EANTIAGAIADIFDALIATLSETRLEPDLDDLLWSTVNLFHRAADRVQRELDDNELVQ
                     KRCQKQQDGSEIRSVELERLIAEGLTLIERRNSMELFRDQAADHFERHTGSCWRPRSG
                     SMVNHRTMTAAMIDSRDFLAAKRRSETEVLLPAGAKIAFTGGLEFNDHRAIWDTLDKV
                     RAKHSGMVLLHGGSPKGAERIAACWANNRKVPQIAFKPDWARYANAAPFKRNDQILDV
                     FPIGVIVFPGSGISANLADKAKRFGIPVWKFGGA"
     gene            complement(3689..4744)
                     /locus_tag="VM910_11795"
     CDS             complement(3689..4744)
                     /locus_tag="VM910_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVI63249.1"
                     /translation="MGSQAAELARRLARDAEAVCRRYLSNGRRQGRYWVVGDVANTPG
                     RSLFVRLTGPESGKGGAGKWTDAATGEHGDLLDLIALSMGLDRFSDVLDEARAFLSLP
                     RVPPPSDCRTPHTPAPIGSPESARRLFAASRSISSTIAEAYLRNRGITAFDETAALRF
                     HPRCYYRPDADAPTEAWPALIAAVTDLSGTITGAHRTWLDPSGGDKAPVDTPRRAMGH
                     LLGHGVRFGTAADVMAAGEGIETMLSLRSVLPRLPMLAALSANHLATILFPATLRRLY
                     VARDRDRAGDVAVTALTARVRTAGIEPITLSPALGDFNEDLRHLGVDELRAALRVQLA
                     PEDVLRFLHAMHAAGPA"
     gene            complement(4755..9102)
                     /locus_tag="VM910_11800"
                     /pseudo
     CDS             complement(4755..9102)
                     /locus_tag="VM910_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
     gene            complement(9322..11376)
                     /locus_tag="VM910_11805"
     CDS             complement(9322..11376)
                     /locus_tag="VM910_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVI63250.1"
                     /translation="MATAKITLSASRDIPFNKLVLSQSNVRRVKAGISIEELAEDIAR
                     RSLLQSLNVRAVLDADGNETGMFEVPAGGRRFRALELLLKQKRMKKTEPVPCVVRTDG
                     LAEEDSLAENVQPAALHPLDQFRAFQTLREKGLSEDDIAARFFVSPTVVKQRLKLAAV
                     SQKLLDVYAEDGMTLEQLMAFTVTNDHARQEQVWEALAHSYNKEGAVRAADKRAQFVG
                     VDVYEAAGGVVMRDLFEHDDGGWLQDPALLDRLVMEKLKAEAETLRTEGWKWIAVATE
                     FPYGHTAGLRRLDGRMVDPTDEERATRETLRAELDRLEAEYADADELPDDVDQRLDEI
                     ETALIAFEERPVIYDPADIARAGAFVSIDSEGKLRIDRGYVNPEDEPPAQPTDTDGHD
                     SAAAEPAIARRPVITVHTNGVSEPQCEPDDDDGLKPLSERLITELTAHRTLALRQALA
                     NDPSVAFAAVLHALCLATYRMSSATCLEISTKSASFNAQPPGLADSASAKAIEARHQQ
                     WAKQLPQDDDDLWPTLVALDRDSQAALFAHCAALSINAVHEPWNRNPQRLAHADLLAR
                     AVNLDMTAAGWKPTVDNYLRRVPKARILDAAREARGEASAQLIDRLKKPEMAKESERL
                     LADTGWLPKPLRTTDAQIGGGSAESEASTDALPAFLADAEDGGPEDPTDAEEPSHAVA
                     AE"
     gene            complement(11520..12506)
                     /locus_tag="VM910_11810"
     CDS             complement(11520..12506)
                     /locus_tag="VM910_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HVI63251.1"
                     /translation="MSRTAARARVGWDRPSLYQEITDKIIAELEADRVPWVQPWGSAV
                     AKASLAMPKNAATQRQYSGINVLILWDAVIEHGFSTQNWLTFRQALQLGGNVRKGERG
                     TTTVYADRFVPDEERERAERDGDEPSAIPFLKRFTVFNTDQCERLPAELTSAPAPVPD
                     GLILPHAEALIAATRADFRIGDDRAFYSPAHDFIQVPRPEAYFEPINWHRTALHELGH
                     WTGHPSRLGRDLSGHFGSPLYAKEELVAEMTSAFVCASLGIVPTVRHTDYIGSWLEVL
                     RKDNRAIVRAASSASKAADYLLAVRLDTGNALAAATAVDHPDATAGPPVFAG"
     gene            complement(12713..13057)
                     /locus_tag="VM910_11815"
     CDS             complement(12713..13057)
                     /locus_tag="VM910_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63252.1"
                     /translation="MDHRISGEIGAEAKITQSGRLEITIMRRCSTPMTVRCRSPPLSD
                     NLPLRPGGSAPADRIADEVISHLTALVGPEVKVTLEIEAEVPNGVPENTVPTVTENSR
                     TLKFDSQGFEKE"
     gene            13090..13551
                     /locus_tag="VM910_11820"
     CDS             13090..13551
                     /locus_tag="VM910_11820"
                     /inference="COORDINATES: protein motif:HMM:NF022840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI63253.1"
                     /translation="MTLTAMRITRLHIENFRSIKKLDIELGQTTVFIGQNNSGKTAIL
                     DAVKIALTRRWGQRGTGFTEYDVHLATESDDPKSSPGVAIELRADELVAGDWPEGLQQ
                     GLDEIVQTDPATGKSFVSLRATCVWVASEKCFQPSWVFLNAARINTYFDGS"
     gene            14373..14657
                     /locus_tag="VM910_11825"
     CDS             14373..14657
                     /locus_tag="VM910_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HVI63254.1"
                     /translation="MGCRNSSGPLLGTKRAIRRPELRQIVPLADTTIYEMEQRGEFPR
                     RFYLTTRCVAWDLAEVEAWLDARRRTSDAEMINRAPAPDVRRRKARPVRQ"
     gene            complement(14659..>15744)
                     /locus_tag="VM910_11830"
     CDS             complement(14659..>15744)
                     /locus_tag="VM910_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI63255.1"
                     /translation="RYASNGISLAEARERCMAARKVVAEGRSPAQEKQRERRRLAEAQ
                     NFGAVAKRWFKGARMAESTRSMRKAIFDRDILPIWKNRLLTEITPDDLRALCNKVKDR
                     GAPATAIHVRDIVKQIYGFAILHGEKVANPADAVGPASIATFVPKDRSLSPAEIRILF
                     QEIEHIATLPTIRLGLRLILLTMVRKSELLDATWDEVDFENAVWSIPKQRMKRGKPHN
                     VYLSRQSLDILIALKTCAANSRYVLPSRYDADAPMSRATFNRITMAVVERAKKKGLPL
                     EPFTVHDLRRTGSTLLNELGFNSDWIEKCLAHEEGRSSRGVYNKAEYEPQRRHMLQEW
                     ADVINSWVQGQKRTPTLYPPSMQLSAS"
BASE COUNT         2874 a         4784 c         5141 g         2945 t
ORIGIN      
        1 cgatttcgcc ctggaactcg tggccggact tcttgaaggt gccgatattt gccatgtcac
       61 ttctccttgg ctgttcgagc ccgcgaccat cgcggcctcg atggcgatct gcaggccgaa
      121 ggcgatcgac gacgcaccct cctcgggccg gagcgtagcg gaggatggcg gctgagagac
      181 tttcttgcct cgcgaggaat gggcgtagcc caggggaaga aagtctcgcc accgccattg
      241 cggcacaggc gatcgaggcg cagccatcct tcggccagat cgagccatcg ggggcagcgt
      301 ggttcgccag gtccgacagc tactcaagga gaacacgtgg cggatatcga gcgatccgca
      361 caagaggtcg cgttcatcgg gcgaagaaaa agaaaccctt gcgtagagat gggcggctct
      421 cttgttgccg tccaaacggg cgacagcgcg cccgcatcat ggttggtatc gggtcaaccg
      481 aggtcgcggc ttggctcgcc gtgaagggct gcaccaaagc gcgacggatc aggattcaac
      541 gaccggattc gcttcaggtc actcgcagca aatatggcgc cgcaatcccc ctggtccgcg
      601 ccgtcagcga ggtgagcggc ctcacgcgag cggcaatgcc cggcccgcat cgttcggggc
      661 agacaaagtt atgcgtgccc acgcgatcgt cgcgaccatg atcgcgccgg tgattcctac
      721 ggtttcccgc caaccttcaa tgggtacgac ggcatcggcg agcccatgtg cggcatgata
      781 gcccgcgatg gcggccggca ccgcaaacaa gagcgcgatt gctgcgcgaa cgggcgtaga
      841 ccgcgacgtt ctaaaggaga gctgtccggc aaacaggacg atgacgctga caattgcagc
      901 gattgcaacc gctgcgattg tcccagaatt gctgtgatag gcggcgagtc ctgcggtgac
      961 gccggcgaac aatggaagtg cgttgacggc cagcgcgaag acgagccagc agagatagcc
     1021 gaggccgata aagctgagaa caatgccgat gatgatcatg gtggtgggtc tccgcacaca
     1081 ggttgacggc ttgcgcctcc gaccaccacc acggcgcaac acaacaatag caggtgtcgc
     1141 tggtcttagg aatcggtgat tggcgaggcg ttcttgaggg gcgctttcgg ccagggcatc
     1201 aaaccaactc agcacctatt cagacgaaat tccgaccggt tgtggctgcc cgcgatagct
     1261 gggtagcgca gcccattttc gcgacggcgg cggggttgtt cttggcaatt tgccgaccat
     1321 atgataggca tattgccgat ggcttttagg gagcttagtg gtgacatccc cgactccgca
     1381 gccagtgatc gaacgggtct tccgcaagct tggcggctat cgggctctgg gctcagacgt
     1441 ggcgtcggag gccgacctcg cccgtatcgt tgaccgcggc attcgcctgg ttgttctcgg
     1501 ctatgttcaa aaggcgggat tctcgaagca ggagatcgaa cgcttgatca ttccggcccg
     1561 tacatggcga caccgtaagg tcaaggggga gcctctctcg acggaagaat cggatcgagt
     1621 cgtaagactg acacgcatcc aggctctggc ggaggacgtc ttcggagatg ccgataaagc
     1681 gaaccgatgg ctgcgcgaag aattgggaat tctcgctggc aaatcgccgt tggaaactgc
     1741 gcggaccgag gcgggcgccc gtctggtcga gcagctcctt gcgaagctcg actggggcgc
     1801 agccgcgtga atgcgtttgt ggcggctatc gagcgagcgg cgcgcacgcg atttcgacgg
     1861 aggctatggc ctttggaacg acggtcgctg gaatagtcgc ggtcgtccag taacctattg
     1921 ctcgaccgta gtgtcgctca ccgctctgga gaagcgggtc catgtgagcg atccggctct
     1981 gctgccgcca caggcgctcg tcgcctatga agtgccggat ggcgtttcca ggcggacaat
     2041 cgcgattgat gatttgccgc tggattggac tttgcgtgag acccacaccc agaccatcgg
     2101 cgaccaatgg ctcgattcgc agacggaggt gttgcttttc gttccctctg tcatcgtgcc
     2161 catcgcaaac gtccctgatc gcaacgtgct catcaatcat cgcgccgcag aggcggctgc
     2221 aatccacatc gctcaagtca tcccgttcgc cttcgatccg cgattattcg ctccttgatg
     2281 tcgtaggcgc cgctttgcaa gtgctgccgc tcaccaaaga gcaacgacgc cattgagcgc
     2341 aatgttgtca accgcctccg aaaaagcggc gaccgcacgt cgcgcatttt gcgaaatcca
     2401 gcggcgccct aggcgccgcc gaacttccag accgggatgc cgaatctttt ggccttgtcg
     2461 gcgagattcg ccgagatgcc ggaccccggg aaaacgatga cgccgattgg aaacacgtcg
     2521 aggatctggt cgttgcgctt gaacggcgcc gcgttggcgt agcgcgccca atcaggcttg
     2581 aaggcgattt gcgggacctt gcggttgttg gcccagcagg cggcgatgcg ttcagcacct
     2641 tttgggctgc cgccgtgcag cagcaccatg ccggagtgct tggcgcggac tttgtcgagc
     2701 gtgtcccaga tggcgcgatg atcgttgaat tcaaggccgc cggtgaaggc aatcttggcg
     2761 ccggcaggca gcaagacctc ggtctcggaa cggcgcttcg cggcgagaaa atcgcggctg
     2821 tcgatcatcg ccgcggtcat ggtgcgatga ttgaccatgg acccagagcg tggccgccag
     2881 cacgagccag tgtggcgttc aaagtgatcg gcagcctggt cgcgaaacag ttccatgctg
     2941 ttgcgacgct cgatgagggt gagaccttcg gcgatgagcc gttccaactc aaccgagcgg
     3001 atctccgaac catcctgctg cttctggcag cgcttctgca cgagctcatt gtcgtcgagc
     3061 tcgcgttgca cgcggtcggc agctcggtgg aagagattga ccgtcgacca gagcagatca
     3121 tcgagatctg gctcgaggcg cgtctcagag agcgtggcga tcagcgcatc gaagatgtcg
     3181 gctatggcgc ctgcgatagt gttcgcttcc ggcagcggcc gcgggtcagg ctcgtcctgg
     3241 aatgggcgat ggccatgcag ctgcaactca gtgaggatgc ggtcggtcgg cgatgacgcg
     3301 tgtagcgatg agaattcgag ttcgtcgcga tcggtcgtca tgatgatgct ccttctcggg
     3361 gtcggccacg cgcaacagcg gccttcacgg cgatcgccag acggcaggcg gaccgggcct
     3421 gcaccgccga gtttttgcga tagcggccgg agcgcagcgg aggacggcgg gcggcggcta
     3481 ttttgcttcg cgctgcaaag ccggagatct gcttgtcggg ctgcgtggac agagtcgctc
     3541 tccggcggcg gaaaatagcc gccgctcgcc gttgcgggct cggtccgctt gccgtccgat
     3601 cgccctcttg aaggtcgcgt cgcgctgctt ttcgagaaga gaggatcacc gggaaccggt
     3661 cgcgacgtcg agggccggat cgcatgcgtc atgccggccc ggcggcgtgc atggcatgca
     3721 ggaagcgcag gacgtcttcc ggggcaagct ggacccgcaa ggcggcgcga agttcatcga
     3781 cgcccaggtg gcgcaaatct tcgttgaagt caccgagcgc cggcgacaaa gtgattggct
     3841 cgatgccggc cgtgcgtacc cgcgcggtca aggccgtcac cgcaacgtcg ccagcacgat
     3901 cgcggtcgcg agcgacgtag agccgtcgca gcgttgccgg gaacaggatg gtagcgaggt
     3961 gattagcgga aagcgccgcg agcatgggca ggcgcggcag gacgctgcgg agcgacagca
     4021 tggtctcgat gccttcgccg gcggccataa cgtcagctgc ggtgccaaaa cgaacgccgt
     4081 gaccaagcag atggcccatg gcgcgccgcg gcgtatcgac cggcgccttg tcgccgccgg
     4141 acggatcgag ccaggtgcga tgggcgccgg tgatcgtgcc agagaggtcg gtgacagcag
     4201 cgatcagcgc cggccaggcc tcagtcggcg catcggcgtc gggccgatag tagcagcgcg
     4261 ggtggaagcg tagcgccgcc gtttcgtcga aagctgtaat gccgcggttg cgcaaatagg
     4321 cttccgcaat ggtgcttgag atcgaccgcg acgccgcgaa taggcggcgc gccgattccg
     4381 gcgatccaat cggagccggt gtatgtggtg tgcggcaatc agatggcggt gggacgcgcg
     4441 gcaggctgag gaacgcgcgc gcctcatcga gcacatcact gaagcgatca agccccatgc
     4501 tgagtgcaat tagatcgagc aggtcgccgt gctcgccggt ggcggcgtcg gtccatttgc
     4561 cggcgccgcc cttgccgctc tccggaccgg tgaggcggac gaagaggctg cggcccggtg
     4621 tgttggcgac gtcgccgacc acccagtagc gaccttggcg gcgaccgttg gagaggtagc
     4681 ggcggcacac cgcctcggca tcacgcgcga ggcgacgtgc cagttcggca gcttgcgagc
     4741 ccatggcgcc cacctcacgc ggccgtcttg tcggcgaggc gaacgagcga atagcgctcc
     4801 atgaggtgag cgagaattgc cgcgccggcc gtgccggtcg gcacgaacag gcgaagcttc
     4861 caggagatga tctccgacat gagaccgatg gctttcaggc gctcgaccat gccgtccgag
     4921 aacccggaca gctcgatgcg gaattcgccc atgaccttga cgcggcggag ctccagtccg
     4981 tcctggagct gcaagatggt ccgtccatcg agcacggccg cgaacgcgtc agggggagca
     5041 agcgtcagcg gatcggcctc gaccgcttcg gccagccaag ctggcgagac cagccgcccg
     5101 atgatgcgct caccgtcatc ggtctgcagc cgatagacgc gcatcgagtc attcggcagc
     5161 cgtttccaga tcggcagcag gagcccggtg acaatatgga ggctgctgtc ggtgaactcg
     5221 ggcaccgctg cgatctcaac ctcccaggcg tgagtaaatt cagcgcgatc ggcctcacgc
     5281 caatggctgt gcgccagatc gtcgaccgga atggccggac gctccatcgg tcgaagcaga
     5341 cgcacccggc gttcaacagt gccgtcatcg cgcatcaggc tcggtgctgg cacctgcacg
     5401 gcggcacgtc tggactggtc gttgaccagg agtttggcgt gccgatcacg tgcgcgcttc
     5461 agagcctcgg caagggtgaa cggccgattg cgatcccggc gggtgatggt gaagatgctg
     5521 gtctcggcgc cgctctgcgg atgggtgtag agcgtgcggc gatcggtgac gaggaggctc
     5581 tcgacgatga tggtctcgac gccgatgtcg taggtgcccg acgccatggc gccctcgacc
     5641 ttggcccgca gcacatcatc gaacacgccg aacagggtgt tctgcagatc aatggtgagc
     5701 gccagcagcc gattgaggaa ggtggtgatc ggcggtagat cctcgcgcaa gctgccgtcg
     5761 ttatcggtca ggcgcaggct tgtcgcctcc tcaaacattt gcagcgtgca gccctcgacc
     5821 ttgccggtga agatgaggag atagagctgg cgcagcgccg cccgcgcgta caccgattcg
     5881 agattgtcat cggcgcggaa caaaccctgg ccgcctgtct gccgctggcc tttggtgatg
     5941 gcgccgagcg tgtcaagccg ccgggcgatg gtggacagaa accgcttctc ggctttcacg
     6001 tcggtggcga ttggccggaa cagcggcggt tgcgcttggt tggtgcggtt cgagcggccg
     6061 aggccctgga tcgcggtgtc ggctttccag ccggcctcca gaaggtagtg cacgcgcagg
     6121 cgctggttct tggcggcgag atcggcgtgg taggaacggc cggtgccgcg ggcgtcggag
     6181 aagacgagaa tgcgcttctt gtcatccatg aaggcctgcg cctcggcgag attggcggaa
     6241 gccgcccgtg cctcgacggc aaagcggtcg agacctcctg ccatggtctt gcgcacgatg
     6301 cgccgcgagc gaccggtaat ctccgcgaca ttgccggcgc caaagcgctg gacgatctga
     6361 tcgagtgcgc cttgcaccgg aggcagtgac gccagccgct cgatcatgcg gtcacggcgc
     6421 tcgagcgcct cgcggcactg caggctcccc gtcgcgatag accggccgag acgagagatt
     6481 gccctcgccg tcggtgaacg gctcatagag ctgggtcgga aagccgtgcg ccagatagtc
     6541 gaggacgtat tcgcgcggtg tgatgtcgac ctggacgtcg ccccactcct cggtcggaat
     6601 ctcggcgagc cggcgctcca ccagcgcctc gcccgtggag acgatctgga tgacggcggc
     6661 gtgcccgagc gcaagatcgc gctccacggc ggcgatcagg gtcggcgtct tcatcgccgt
     6721 gatcagatga ttgaagaagc gctgcttggc gctctcgaag gccgagcgcg cggccgactt
     6781 ggcctggctg ttgagcgtgc cgctctcgcc agtgacgttg gcggcctgca gcgccgcatt
     6841 gagattgtta tggatgacct ggaaggcgcc cgcataggca tcgtagatgc gggcctgctt
     6901 gtccgagagc tggtgctcga cgagctcgta ctcgatgccc tcgaaggaga gcgagcgggc
     6961 ggcatagaga ccgagtacct tgaggtcgcg ggccatcact tccatggcgg cgacgccgcc
     7021 ggcttcgatt gcctcaacga actcggcgcg ggtggcgaag gggaagtcgg ctccgcccca
     7081 gagcccgagg cgttgggcat aggcgagatt gtggactgtg gtggcgccag ttgccgacac
     7141 gtagagcaca cgcgcgttcg gcaaggcgtg ctgcagcagg cccgccctcc cctgtagcga
     7201 cggcgcgcgc tcgccgcgtt cggatttttc gccggccgcg ttctgcatgg cgtggctctc
     7261 gtcaaagacg acgactccat cgaagtcgcg gcccaaccaa tcgacgatct gttgcacgcg
     7321 cgaaagcttt tcctcgcggg cgtcggaccg tagcgtggcg taggttgtaa aaaggatgcc
     7381 ttgctccagt cggatcggtg cgcccgggcg gaagcgcgac agcggcgtga tgagcagccg
     7441 ctcctggccc agcgccgacc agtcgcgttg tgcatcgtcg atcagcttgt cggattttga
     7501 gatccagaca gcgcggcggc ggcctttgag ccagttgtcg agcagaatgc ctgcgacctg
     7561 acggccctta ccggcgccag tgccgtcgcc gagaaaccag ccgcggcgga agcggacggc
     7621 attgtcggtg tcgtccgctg cagcggagac gacgtcgaaa gtctcgtcga ccgtccacgc
     7681 gccggcaaga tatccggtat gcgcgtcgcc cgcatagatg acgctctcga gctgcgcatc
     7741 ggacaccaag ccatccgcga taaccgccgg cggaagatgc ggccgatagc tgggctttgg
     7801 tggcgcgacc gatgccatgg cggctgattg cacgagccgt gtcggatgtg cctgcgctcc
     7861 aggaatacgg atcgattgaa gcgcgtattc ttcgtagagc gcatcggtga tctggccgtc
     7921 cgactgcggc gcccagtcga tggtttcgta ggtgagttcg acggcagccg gctcagctgc
     7981 cgccacagaa tacttagtcg gggaagggag aatacgctgc gcggggcgtc ttcgccgagc
     8041 aatccgctgc atcctcgccg cgatggcagc tgctgccgtc gcggcggggc gcggcggaac
     8101 gagcgctgtc acccagtcga gcagcactcc cgcgtcggat gccattcctg gcgaagcgga
     8161 aaatgctgtc ggatcgtcag ccggtacgcg gtcgatgacg gtcagacgcg tctcagtggt
     8221 ggtgccatga cgggcataga cgtgaccgtc gatcgatgct gagaacacca gtcggctgcg
     8281 ttcctgcagc tggacgaaac catcgcgcca tgccggattg tcgggagaaa ggctggcgcc
     8341 cgtgatggcg accaggcggg caccgtcggc tagacgcgtc agcgccgaca tgatgtgccg
     8401 gaacgcggca tcggcgaccc ggccctcaac atgcactgcc accgagaacg gcggattcat
     8461 cagcacaatg gtcggacaga tggcggcatc gagatgatcg tggatgtgag cagcgtcata
     8521 ccgggtgacg ttaatggcgc cgaacaactc atcgagcagg caggcacgtg cgtcagcgag
     8581 ctcgttgaga tgcagctgcg caccggcgac ctcggcatga atcgcaagga gccccgtgcc
     8641 agccgaaggt tcgagcacga gatcgtctga cgtgatcgcc gccgccacgc tgacggcgaa
     8701 accaagctcg atcggtgtag agaactgctg gagtgcttcg ctctcctccg agtggcgtgt
     8761 atgcgaagga acgagcgccg cgagtttttt cagcatcgcg aggaatacgc agcgcgatac
     8821 tgcgcgcttg cgcatcgctg gcccgaactt gcgcaagaac agaatctgcg ccacctcgca
     8881 ggcgtcgtag gcggtcttcc agttccacgc gccttccgcg tccgagccgc cgactgcgat
     8941 gctcatcgaa ttgcgcagcg tctgcgcatc gactgcgcgt ccgcgttcaa gatcggcgag
     9001 gagcaacctg gccgcggcga gaagtgctgc gccggtattg gatgacgaag ctggaggtgc
     9061 ggcagagcta tccgccgcca gcgcggccgg cgagagaagc atggaattca tgggagagaa
     9121 tccaggagag cgaggatggg atgagccgcc gagcgctctc tcgacctcag ccggctcaaa
     9181 cccgttccgg ccgtcctctc actctcggcg cagcaagctc gctcgccgtc tgctgcgccg
     9241 gcacgaaaaa agcccggcgt aagagccggg ctccggaggt tggaagaatg cggggcggct
     9301 agcgcgcccc gctgcgatga gtcattccgc cgcgacggcg tgagacggct cctcggcatc
     9361 ggtcggatcc tccggaccgc cgtcttccgc gtcggcgagg aatgcgggca gagcgtccgt
     9421 tgaggcttcc gattcggcac tgccgccgcc gatctgggca tccgtcgtgc gcaacggctt
     9481 cggcagccag ccggtatccg ccaacagccg ctcggattct ttggccatct ccggcttttt
     9541 gagacgatcg atgagctgcg cagaggcctc gcccctcgct tcgcgcgctg cgtcgagaat
     9601 gcgcgccttc gggacacggc ggagataatt gtcgacggtc ggcttccagc cggctgccgt
     9661 catgtcgagg ttcacggcgc gagcgagcaa gtcggcatgg gcaagtcgtt gcgggttgcg
     9721 gttccacggc tcatgcaccg cattgatgct gagcgcggcg cagtgcgcga acagggccgc
     9781 ttggctgtcg cggtccaacg cgaccagcgt cggccagaga tcatcgtcgt cttgtggcag
     9841 ctgcttcgcc cattgctggt ggcgcgcttc gattgccttg gcagaggcgc tgtcggcgag
     9901 ccccggcggc tgggcattga agcttgcgct cttggtggag atttccaggc aggtagccga
     9961 cgacatccga taagtggcca ggcagagcgc atgcagcact gcggcaaacg cgacgctcgg
    10021 atcattggcc agcgcttggc gcagcgccag cgtccggtgc gccgtcagtt cggtgatgag
    10081 ccgttccgac agcggcttga ggccgtcgtc gtcatcgggt tcacattgcg gctccgacac
    10141 tccgttggtg tgcacggtga tgactggtcg ccgggcaatg gctggttcgg cggccgcaga
    10201 gtcgtgtcca tcggtgtcgg tcggctgcgc cggcggctcg tcctccgggt tgacgtagcc
    10261 gcggtcgatg cggagcttgc cttcgctgtc gatgctgacg aacgcgccgg cgcgtgcgat
    10321 atccgccggg tcgtagatga ccggacgctc ctcaaacgcg atgagcgccg tctcgatctc
    10381 gtcgagccgc tggtccacat cgtccgggag ctcgtcggca tcagcgtatt ccgcctcgag
    10441 gcggtcgagc tcagcccgca aggtctcgcg ggtcgcgcgc tcctcgtcgg tcggatcgac
    10501 catcctcccg tcaagccggc gcagaccagc ggtgtggccg tacgggaact cggtcgcgac
    10561 cgcgatccat ttccaaccct cggtgcgaag cgtctccgct tcggctttga gcttctccat
    10621 caccaggcgg tcgagcagtg ccgggtcctg aagccagccg ccatcgtcat gctcaaacag
    10681 gtcacgcatg acgacgccgc cggcggcctc atagacatcg accccgacga attgcgcccg
    10741 tttgtcggcg gcgcgcaccg cgccttcctt gttgtagctg tgggcgagcg cctcccagac
    10801 ctgctcctgg cgtgcatggt cgttggtgac cgtaaacgcc atcagctgct ccagtgtcat
    10861 gccatcttcg gcatagacgt cgagcagttt ttgcgagaca gcggcgagtt tcagccgctg
    10921 cttgacgacg gtcggactga cgaagaaccg cgctgctatg tcatcctcgc tcagcccctt
    10981 ctcgcgcagg gtctggaaag cgcgaaactg gtcgagcgga tgtagtgcgg cgggctggac
    11041 gttttcggcg agcgagtcct cctcggcgag gccatcggtc cgtaccacgc acggtaccgg
    11101 ctcggtcttc ttcatgcgct tctgcttcag cagcagctcg agggcgcgga agcgacggcc
    11161 gccggccggc acctcgaaca tgccggtctc gttgccgtcg gcatccagca ccgcccgaac
    11221 gttcaagctc tgcaggagcg agcgccgggc gatgtcttcg gcaagctcct cgatggagat
    11281 gccggccttg acccggcgga cgttggattg gctcagcacc agcttgttga aggggatgtc
    11341 gcgcgacgcg ctgagcgtga tcttggcggt tgccatgtcg atattctccg cgacgggcgg
    11401 ctgagagcct ttctctcgac ctccaacccg tcgcggacat ggcgccgtgt ttcttgaaca
    11461 cttcaacggt cgaccgactt ggagtgggtg aagcgtgtcg atcgcgggca tttcggcgct
    11521 cagccggcaa ataccggcgg gccggcggtc gcatccggat ggtcgacagc cgttgcggct
    11581 gccaaggcat tgccggtgtc aaggcgaact gcgagaagat agtcggccgc cttcgaagcg
    11641 ctgctggcgg cgcgcacgat ggcgcgattg tccttgcgca acacctcaag ccaggagccg
    11701 atgtaatcgg tatggcgcac ggtcggcacg atgccgagtg aggcgcacac aaaggcgctg
    11761 gtcatctccg cgaccagttc ttccttcgcg tacagcggcg agccgaagtg ccctgacaaa
    11821 tcgcgcccaa gccgggacgg atgaccggtc caatgaccaa gctcgtgcag cgcggtgcgg
    11881 tgccaattga tgggctcgaa ataagcctcc ggccgcggca cctgtatgaa atcatgcgcg
    11941 gggctgtaga aggcgcgatc atcgccaatg cggaagtcgg cgcgggtcgc ggcgatcagc
    12001 gcctcggcgt gcggcagaat gagaccgtct ggtaccggtg cgggagcact ggtcaattcc
    12061 gcagggaggc gctcgcattg atcggtgttg aacaccgtaa accgcttgag aaatgggatc
    12121 gcgcttggct cgtcgccgtc gcgctcggcg cgttctcttt cctcgtccgg cacaaagcgg
    12181 tcggcgtaga ccgtggtagt accgcgctcg cctttgcgga catttccgcc gagctgcaag
    12241 gcttggcgga acgtgagcca attctgcgtg ctgaagccgt gctcgattac tgcgtcccag
    12301 aggatcagca cgttgatccc ggaatattgg cgctgagtgg cggcgttctt cggcatcgcg
    12361 agcgacgcct tcgcgaccgc gctgccccag ggctggaccc acggcacccg gtcggcctcc
    12421 aactcggcga tgatcttgtc ggtgatttcc tgatagaggc tcggccggtc ccaaccgacg
    12481 cgagcacgag cggctgtgcg tgacatcgcg gatctccgcg acgggcgccg cgagcctctc
    12541 tcgcggaccc aaacccgtca cgcccgatcc ggcgaaactc tcactctaat cgcttcgcgt
    12601 gtttcaaaat gcgcgaggtt gggtgcaccg atcaccctta ccccccggtg acgcggttat
    12661 gcccacctag aaaactcggt ggtgttttct cgatgattta gggccccctc gactattcct
    12721 tctcaaatcc ctggctgtcg aacttcaagg tgcggctgtt ctctgtgacg gttggcaccg
    12781 tattctcggg aacgccgttc ggcacctcag cttcgatctc aagcgtaact ttcacttcgg
    12841 gcccgaccaa cgccgtaagg tgcgaaatca cctcgtcggc gatgcggtcg gccggtgcgg
    12901 acccaccggg ccgaagaggc aggttgtctg agagaggtgg gcttcgacac cgcactgtca
    12961 taggtgtaga gcaacgccgc attatggtga tctccagccg cccagattgc gtgatcttgg
    13021 cttccgcacc gatttccccg ctgatacggt ggtccacaat tgcgctcctg gcgagatgat
    13081 aaaaattcag tgacactgac ggcaatgcgt attacgcggc tccacatcga aaatttcaga
    13141 tcgatcaaga agctcgacat cgagttgggc cagacgacag tttttattgg gcaaaacaat
    13201 tctgggaaga ccgctatcct cgatgcagtc aaaatcgcac tgacgcgcag gtggggtcag
    13261 cgtggtacag ggtttactga atacgacgtg catctagcga ccgagtccga cgatcccaaa
    13321 tcgtcaccag gcgtcgcgat agagcttcgc gctgacgaac tggtcgccgg agattggccg
    13381 gaaggcttgc agcagggcct tgatgaaatc gtccaaacgg accccgccac gggtaaaagt
    13441 ttcgtcagtt tacgcgcaac ctgcgtctgg gtcgcatccg aaaagtgctt ccagccttcc
    13501 tgggtattcc tcaacgctgc acgtatcaac acttatttcg atggcagttg aaataaatag
    13561 tccgcgcttt tgccggttgg gttgccggca tgactcatcc gtgagcgcct agtgcgatgt
    13621 ccggaattgg cccgactccg acgtgctgcg atgtccgcat gaatgcgcga tcgtcgagct
    13681 tgacccgcac cgggatgcgt tgcgccaagc ggacccagct gaaggttgga ttgacggacg
    13741 ccaacagctc gctgctcaga aagcagccgc agagtatcgc caccaaaaga acccgcattc
    13801 ccaaaccctg cccgcaacgc gcttgcctcc gagtcgacaa tcctcctccg gaaatcgccc
    13861 gagtgaggag atcgcctccg ccaatcatca ggctttgacg tcacgggcga gtatcgttcc
    13921 ccatatcgtt ccccgtgacg gcttctgctg cgaagaaagc tggtcggagt ggcaggattt
    13981 gaacctgcga ccccatcgtc ccgaacaagg tgctctacct cggcaccact gattccgccg
    14041 ctgctcgcgg ttttggcgag cttgtcacag gaacgaaagc gaaagaaaag tagaacaggc
    14101 gtgtgcaaaa atcgcacacc gctctcgcca atgcgttgat caagctaatc tttcgctcca
    14161 agccatcatg gaatggactg gcccactgat aagagctgcg acggagtgca gttgtccgat
    14221 ttgcacgctg tggccgccga cgacccgacg cccgttgaat ggcccttccc tatcggcgcc
    14281 ctctcatgtg gtgtcgaggc catattcgtc ggatgtcagc gatcgtgaga gagactaagt
    14341 cagccgtgcc attgcaaacg gtttctggcc tagtgggctg cagaaactca tcagggccat
    14401 tactcggcac taaacgagcc atccggaggc ctgagttacg ccagatcgtg ccgcttgccg
    14461 acacaacgat ttacgagatg gagcagcgag gggaatttcc tcgccgattc taccttacaa
    14521 cacgttgtgt agcttgggac ctcgcagaag tggaagcatg gctcgacgcg cgtcggcgca
    14581 catccgatgc cgagatgatc aaccgtgccc ctgctcccga tgtacgtcgg cgaaaagcga
    14641 gaccggtccg gcaatagctt aggacgccga caactgcatt gacggtgggt agagagttgg
    14701 cgtgcgcttc tgcccctgga cccaggaatt aatcacgtca gcccactctt gcagcatgtg
    14761 tcgccgctgc ggctcatatt cagccttgtt atagacgccg cgtgacgaac gcccctcttc
    14821 atgagccagg cacttctcga tccagtcgct gttaaaacca agctcattca ggagggtcga
    14881 cccagtgcga cgcaagtcat gcacggtgaa tggctcaagc ggcagaccct tctttttagc
    14941 acgctccacg accgccatcg ttatgcggtt gaatgtagcc cgcgacatgg gagcgtctgc
    15001 gtcatagcgc gacggcagca cataacgtga gttggctgcg caggtcttga gtgcaatcaa
    15061 gatgtccagc gactgccggg agagatagac attgtgtggt ttgccgcgct tcattcgctg
    15121 cttcggaatg ctccagactg cattttcaaa atccacctca tcccatgttg catctagcaa
    15181 ttcgcttttg cgaaccattg tcagcagaat tagtcgtagg cccaggcgga tcgtaggcag
    15241 tgtggcgatg tgctcgatct cctgaaacaa gatccggatt tcagccggcg aaagcgacct
    15301 gtcttttggc acaaacgtgg caatcgaagc gggaccaacc gcgtccgccg gattcgcaac
    15361 cttctcgcca tggaggatcg cgaaaccata aatttgtttg acgatatcgc gaacatgaat
    15421 ggccgtcgct ggcgctccac gatccttcac tttattgcag agtgcgcgga gatcgtccgg
    15481 cgtgatctcg gtcaagaggc ggttcttcca aatcggcagg atatcgcgat cgaagattgc
    15541 ctttcgcatc gaccgagtgc tctctgccat gcgtgctccc ttgaaccaac gcttggcgac
    15601 ggcaccgaaa ttctgagcct cggcgagccg ccgcctttcg cgctgttttt cctgtgctgg
    15661 tgatcttccc tcggcgacga ctttccttgc ggccatgcac cgctcgcggg cctcggctag
    15721 ggagatgccg ttggaagcgt agcg
//
LOCUS       DATLJT010000510         1984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3705892,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000510 DATLJT010000000
VERSION     DATLJT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3705892"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..1650)
                     /locus_tag="VM910_11835"
     CDS             complement(379..1650)
                     /locus_tag="VM910_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HVI63256.1"
                     /translation="MTTSTMMTTAIPRAPVGKAWEEVSASFDRFCLAAGIETLGAMME
                     ADVELACGTRHSRSEDRRGHRWGRTTGKIGFHAGKVAIERPRARGLDGKELPLPSWEQ
                     AVEEDWLGRWAMNLMLINVSTRKFRRAVRLPEGDVPAPAGSGVSKSAASRHFVALSAA
                     RMQEWMSSELSTLDLLVVQIDGIHMTGELVLVAALGIDCNGVKHPLGLIEGATENAAV
                     VQALIDNLVERGLDPTVPRLFIIDGSKALSKAIRRSFGREALIQRCQIHKARNIMERL
                     PKPMHAAVRRVLRQAWEMDDADKAEKLIRNLARRLEHDAPGVSASILEGLDEILTVTR
                     LGLPKELRRALACTNIVENVMSTVRRVCRNVKRWRSASMALRWTAAAMQEAAKGFRRL
                     KAHKQLPALRAALEAHRNSSNGVLVRQANAA"
BASE COUNT          327 a          668 c          590 g          399 t
ORIGIN      
        1 ccgcagcgga tcgctccgcc ttgttcgtcg gcttcacggc tactatggcg gagtctgact
       61 tcccacgccc gtgcatcatc agctacggct cctcgccttc ccgatgcgga ccgcttgagt
      121 aggtagcggc cagacgtggg atctcccggt tcccgtataa agagcgtccg cacatgccag
      181 ggtcgtcgac cacgccgggc cgttcgggcg ctcgcgtata tcgcgcccgt ccgtgttgcc
      241 ttccgctcgg acgacggcgt cggcacccgg gataagtttc tttcgcggct caatggctgg
      301 cctatgcgtt cccctgccga cgctggagat gtcccgccgt ttgttgaaca ttgcgagcgg
      361 tcgttgccat ggttgacgtt acgcggcgtt ggcttggcga acaaggacgc cgttgcttga
      421 gtttcggtgt gcttccagag cggcccgtaa cgctggaagt tgtttgtggg ccttcagtcg
      481 tcggaatcct ttggcagctt cctgcatagc ggccgcggtc cagcgcagcg ccatcgaggc
      541 cgatcgccat cgcttcacgt tgcggcagac gcgacgtacg gtgctcatga cattctccac
      601 gatgttggtg caggcgagcg cccgacgaag ctctttcggc aggccgagcc gcgtgacggt
      661 gaggatttcg tcaaggccct ccagtatgct cgccgacact cccggagcgt cgtgttccag
      721 ccggcgggca agattgcgga tcagcttctc ggccttgtca gcatcgtcca tctcccaggc
      781 ttgccgcagc acccgccgca cggcggcgtg cattggcttt ggcagccgct ccatgatgtt
      841 gcgagccttg tgaatctggc accgttgaat gagcgcctca cgcccgaagc tgcgccggat
      901 cgctttcgag agcgccttcg atccgtcgat gatgaacaat cgcggcaccg tcggatcgag
      961 cccgcgctcg accagattgt cgatcagcgc ctggaccaca gcggcgttct cggtcgcgcc
     1021 ttcaatgagc ccaagcgggt gcttcacgcc gttgcagtca atgccgagcg ccgccaccag
     1081 caccagctcg ccggtcatgt gaatgccgtc gatctgcaca accaagaggt caagtgtgga
     1141 cagctccgac gacatccatt cttgcatgcg ggcagccgag agcgccacga agtggcgtga
     1201 cgccgccgac ttcgataccc cgctccccgc cggcgccgga acgtcaccct ccggcaaacg
     1261 gaccgcacgc cggaacttgc gcgtcgacac gttgatcagc atcaggttca tcgcccaccg
     1321 gccgagccag tcctcttcga ccgcctgctc ccagcttggg agtggcaatt ctttcccgtc
     1381 gagcccgcgg gcccgaggcc gctcaattgc gaccttgccg gcgtggaagc cgatcttgcc
     1441 ggtcgtgcgc ccccagcgat ggccgcgacg gtcctcgctg cgcgaatgcc gtgtcccaca
     1501 ggccagctcg acgtccgctt ccatcatcgc acccaacgtc tcgatcccgg ccgccaggca
     1561 gaaccgatcg aaggacgcag acacttcttc ccacgcctta cccacaggcg ccctcggaat
     1621 cgcagtcgtc atcatggtag atgtcgtcat ggcgttgctt tcctttcgtg gaatcagcac
     1681 cccgagccta ccggctcaag gcgagcaacg ccgccccttc tagttcaaca tcggcaggga
     1741 caattcctcg tcgcccggcg cgatcatgtt cgacgacttg gtcggcaaag cttgacgtgc
     1801 gcttctggtt gagtgcgcga agcgcgaccc atgatttgaa tttcgtcaag gctgcgcacc
     1861 gggggacctg acgtgtcgta aataccgaaa attgaattga ctcggcttct gggcgggttc
     1921 cagatgccgc cgggccatct gggaaacgcc ttttttaagc cgggacgtgc cgaaacccct
     1981 gctc
//
LOCUS       DATLJT010000511         5337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_260040,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000511 DATLJT010000000
VERSION     DATLJT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260040"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..736)
                     /locus_tag="VM910_11840"
     CDS             complement(176..736)
                     /locus_tag="VM910_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63257.1"
                     /translation="MLAALGGAAAAWPLAARAQQAPKMLRVGTVSAQPRSASVWMAFE
                     QRMVELGYEQGKNFALEIVPAASTQGFERGYRELAARNIDIVLASGPEIALKSALAAG
                     RSLPIVMIAIEYDLARQDFDLRKATPASVLAKAGPGIRFNEHIEGDGPTVFDHSCKLG
                     LEGIVSKRLGSPYRSGRSRDWVTGSG"
     gene            1516..1770
                     /locus_tag="VM910_11845"
     CDS             1516..1770
                     /locus_tag="VM910_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63258.1"
                     /translation="MVAAVAMAVCVAAVAEDSMEAADAEDSMEAADAEDSMEAADAEA
                     VSLGAAAAAVVVAAAAAAAESAGHGRGLAGAGASLHLRLG"
     gene            1929..2135
                     /locus_tag="VM910_11850"
     CDS             1929..2135
                     /locus_tag="VM910_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63259.1"
                     /translation="MNFRWWRKWDAYRQCGVYAAKILKGANPSDLPVLQSAKFELVIN
                     LKTAKTLGLKIPETLLVRADEVIE"
     gene            complement(2329..2496)
                     /locus_tag="VM910_11855"
     CDS             complement(2329..2496)
                     /locus_tag="VM910_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63260.1"
                     /translation="MRPDAIPPDQLRDLVETYINLHLPQAKLEVLKAAEESERDIITR
                     LVGKIARKRLR"
     gene            complement(2854..4062)
                     /locus_tag="VM910_11860"
     CDS             complement(2854..4062)
                     /locus_tag="VM910_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63261.1"
                     /translation="MKLTEKIVATLQLPTGVNERLFGDDDLTGLSLRLRRGANSIAKS
                     WVYRYMVAGAPRKFTFDFAGHNLAAARARAGDLQARVRLGHDPAQERAQTRADVQQTV
                     VATLQTYLPQKKLTLRPRSYVETERHLLVDFAPLHATPLRLVTARDVSARYLAIASTK
                     GRTTATNALRALSAFFDRALRQGLIERNPCLGVERFDVRKRDRVLTAAEIKALWDATS
                     GSDDYSAIVRLLLLTGCRANEIAKLRWDEIKSDRIILPAERTKTGKEHSIPLTGGMRT
                     ILGGRNRRHDKEHIFGRSHDRPFTGWSESKAALDARLKAAGHKLKPWVNHDLRRTIAT
                     GMGELEIAPHVIEAVLNHASGFRAGVAGTYNRAKLEKPMRHALEAWDAHIGEIVEGRI
                     AGDRVVPLRA"
     gene            4172..4465
                     /locus_tag="VM910_11865"
     CDS             4172..4465
                     /locus_tag="VM910_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63262.1"
                     /translation="MLMRSHAVTAQQILQLMLIGQAMARPFAAPPGIPADRKAALIAA
                     FERTTKDPDFLAEAEKLNFEVNPVSADKLDALLAEAYATPKDVIAKAAKAMSD"
     gene            4712..>5337
                     /locus_tag="VM910_11870"
     CDS             4712..>5337
                     /locus_tag="VM910_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI63263.1"
                     /translation="MTLGKMRKPLSSASVAKLTKPGRYAVGDGVYLQISEWNNKISRA
                     WLLRFQLNGKARHMGLGPYGLVTLAEARAKARDARRLLLDKVDPIEARRAQHRKRLLE
                     TARGKTFRECAEGYIASHEAGWRDPRSHKQWVRSLVAYAYPHIGDLSVTDIDTALVLA
                     TLEPIWKAKPETASRVRGRIESILDWAKARGYREGENPARWRGHLDNL"
BASE COUNT         1016 a         1568 c         1670 g         1083 t
ORIGIN      
        1 gaccgccata cctgcggcaa gaattgcgtt gtattccggt cccccaatct gcttccaaga
       61 gttcggattt tgcgagggat cgtcgtaacg gataatcgcg gtgatgccag ccttcttcaa
      121 ggccgcaatt ttgctggtac aatcatctgc ggtatcaaaa accgtatttt ttcactcaac
      181 cactccctgt aacccagtcg cgcgagcgcc cagaacggta gggcgagccg agccgcttcg
      241 atacgatgcc ttcgagcccg agcttgcagg aatggtcgaa gacggtcggg ccatcccctt
      301 cgatatgctc gttgaaccgg atgccggggc cagccttggc cagcacgctc gcaggcgtgg
      361 ccttgcggag gtcaaagtcc tgccttgcta ggtcatactc gatcgcgatc atcacgatcg
      421 gcagcgagcg cccggccgcg agcgccgact tcaaagcgat ttccgggcca gaggctaaaa
      481 caatatcgat attacgcgcg gccagctcgc ggtagccacg ttcgaaaccc tgcgtgctcg
      541 cagccggcac gatttccaag gcgaagttct tgccctgctc gtaaccaagc tcgaccatgc
      601 gttgctcgaa cgccatccaa actgacgctg atctcggctg ggcgctaacc gtaccgacgc
      661 gcaacatctt cggcgcttgc tgcgcgcgcg ccgcgagcgg ccaagcggcc gccgcgccgc
      721 cgagtgcggc cagcaattcc cgccgtccga tggtgactat catacgcccc tgccgtgcga
      781 ggtgcgtaga agggaacgat accacgccac gagcgtgctg tccctaacag cgcggcgccc
      841 ggcgtgggtg gagcgtcttg atttcaagag acatattttc ggccgcgccg taggccgtgt
      901 actcataaac gcgggcattg gcccaatgtc cggtcttgga gcaaaccgga cacgccggga
      961 tggcgggaat gacgtgaatg acccatctga gacattggca gcgaaaattg ctgttcggta
     1021 accgccctat cttttttgct cgaagcccga gagggcgtga gagcggaaat ttggtaatag
     1081 ctccggcagc tcggcgatat ttgccgcgat ccgccgcgcc tatgcaattc ctaacttttt
     1141 tgacacttgc gccggggtct aagcggaacg taacgtagca gtatcgattg gttcaatgtg
     1201 ccccaaacgg acgggagaat tgtgccatgt cgaagcgctc tttgaaaagc aaacgtcgga
     1261 gtaaagcctt acccgcgttg agtttcgccg gcgcatctct ctcaatggca agcggcgcgt
     1321 gcgcctcaac tagcgaagcg tcggctaata caccgccgac ctcacagagt cagaatcagg
     1381 aaatctttct cggcgaagag gaaatcttcg acgttagctt gtcgacgttc tatgtctttg
     1441 acaaggaaaa cggcggagca tccccactcg cccaacactt aaaattggcc cgtggcggct
     1501 gcggtggttg cggccatggt tgcggctgtg gccatggcgg tttgcgtggc tgcggtggcg
     1561 gaggattcca tggaggctgc cgatgcggag gattccatgg aggctgccga tgcggaggat
     1621 tccatggagg ctgccgatgc ggaggccgtt tctttagggg ctgcggcggc tgcggttgtg
     1681 gtggctgcgg cggctgcggc tgcggaatct gctggacatg gccgtgggtt ggctggtgct
     1741 ggggcgagtc tacacctccg gctggggtag cttctgtcgc gtcggagcaa tccgcacaga
     1801 cggtggaggt tgcggtaaaa ggtgacggga aaatcgacgg cacgcgagct aaatagcatt
     1861 cggcgctacc gcctcaccga gcagcattgg gtaaaggtca agtgctgagc gtccagagcg
     1921 atctgtgcat gaactttcgc tggtggcgta agtgggatgc ttatcgccag tgcggggtct
     1981 acgccgcgaa gattctcaag ggcgcgaatc cctctgacct gccggtgttg cagtcagcca
     2041 agttcgagct ggtgatcaat ctcaaaactg cgaagacgct tggccttaag atcccggaaa
     2101 cgctcctggt acgggccgac gaagtgatcg aataagattt ttttgctgca cggcatatgt
     2161 cgcctattgg cacatcgcgt caattcgcgg cgacgcagca actcggtcgc tttcggagcg
     2221 aagcggacat agaccgagcc gcgcttacag aaagcggcag cggggcggct cgattcagca
     2281 tccggccctc cccagggcgg cggcactcgc ttcgtcgctc gggatcggtc atcgcagcct
     2341 ctttcgtgcg attttcccga ccaggcgcgt gatgatgtcg cgctcggatt cttcggccgc
     2401 tttcagcact tccaacttgg cttgcggtag atgcaggttg atgtaggtct cgaccaggtc
     2461 gcgaagctgg tcgggcggga tggcgtcagg ccgcattcgc tgcctcgctt gcacaagcgg
     2521 cggaaaaatt ccgtcgcagc gacagtattg cgactctgaa ttcgacggcg ggcatcgtct
     2581 tcggcgagcg ctttggttcg gaatagcgtc gcgcccgatg attgcccgaa gtccttccat
     2641 cgcaatggac ttgaaccact cgtgccggga ttaggctagt cggggcctcg gctggccaca
     2701 ttaggcgccc ttccgccgcc gcagcgcgag gaccttcgcg ggcttccgct cgcccgcccg
     2761 ccggatgccg tcaggtcgca ttcgctgctg cggaaaaatc ccgccggcca aagtccggcc
     2821 gtcgaccggc ccgcttatta ctccagatgt atttcaggca cgcaacggca cgacccgatc
     2881 gccggcgatg cgaccctcaa cgatctcccc gatgtgggcg tcccacgcct cgagtgcgtg
     2941 gcgcatcggc ttctctagct tggcgcggtt ataggtaccg gcgacaccgg cccgaaagcc
     3001 agacgcgtgg ttaagcaccg cctcgattac gtgcggtgcg atctcgagtt cgcccatgcc
     3061 ggtcgctatg gttcgcctta ggtcgtggtt gacccagggc ttgagcttgt ggccggcggc
     3121 cttcaggcgc gcgtccagtg ccgccttgct ctcgctccaa ccggtgaagg ggcgatcgtg
     3181 cgaacgaccg aaaatatgtt ccttgtcatg gcgccgattc cgaccaccga ggatggtgcg
     3241 catccccccg gtgagtggga tcgaatgttc cttgccggtc ttggttcgct cggcgggcaa
     3301 tatgattcgg tcggacttga tctcgtccca cctcagcttg gcgatttcgt tggcgcggca
     3361 accggtgagc aacagcaacc tcacgatagc cgagtagtcg tcggatcccg aagtcgcgtc
     3421 ccatagcgcc ttgatctcgg cagcggtgag cacccggtca cgcttgcgaa cgtcgaagcg
     3481 ctcgactccg aggcaggggt tgcgctcgat cagtccttgc cgcaaggccc ggtcgaagaa
     3541 cgccgacagc gcgcgcaagg cgttggtggc ggtggtgcgc cccttggtgc tggcgattgc
     3601 cagatagcgg gcgctcacat cgcgggcggt gaccagtcgc agaggtgtag catgcaaggg
     3661 ggcaaaatcg accagtaggt ggcgctcggt ctccacgtag ctgcgcgggc gcagcgtgag
     3721 cttcttctgc ggcaggtagg tctgcagcgt cgccacgacg gtctgctgga cgtcggcgcg
     3781 cgtttgcgcc cgctcctggg cgggatcgtg accgagccga accctggcct gcagatcccc
     3841 ggcgcgcgcg cgggcggcgg cgaggttgtg gccggcgaag tcgaaggtga acttgcgggg
     3901 tgcgccggcg accatgtacc gatacaccca gcttttggcg atgctgttgg cgccacggcg
     3961 caggcgcagg ctcagcccag taaggtcgtc atcaccgaac agtcgctcgt tgacgccggt
     4021 gggtagctgc agggtggcga cgattttctc ggtcaatttc atggtccgcc ttggatggct
     4081 tgggtacaac tggggtacaa cacgggtaca atcttatgct gtctcgtgcc cattatggat
     4141 tgataacttg atgtacttgt caagctcatg catgctcatg cggtctcatg cggtgactgc
     4201 ccagcaaatc ctgcaattga tgctgatcgg acaggccatg gcgcgcccgt tcgcggcccc
     4261 gcccggtatt cccgcggatc gcaaggcggc gctgattgca gccttcgagc gcacgaccaa
     4321 ggacccggat ttcctcgccg aggcggaaaa gctcaacttc gaggttaatc ccgtcagcgc
     4381 ggacaagctc gatgccttgc tggctgaggc ctacgccacg cccaaggacg tgatcgccaa
     4441 agccgccaag gcgatgtcgg attgagcgac gttcacggga tgaaatgatc acaaaggccc
     4501 gcgctgcgaa acgtgaggcg cggtcgagga catgggtacc caatccgtaa tcaccgaagg
     4561 cttccataga cttccataac cctccacatt tatgcccacc gttattgact tactagcaac
     4621 acacctttcc aaatgctccg acgaggtgta aagggttccg agtattgttt gtaggcaaga
     4681 ctgtaggagg gaaggtcgag attgaccgtc cttgaccttg ggcaagatgc gaaaaccatt
     4741 atcctcggcc tctgttgcca agctaacgaa gcccggccgc tacgcagttg gcgatggcgt
     4801 ctatctgcaa atcagtgagt ggaacaacaa gatcagccgc gcatggcttc ttcgcttcca
     4861 gcttaatggc aaagcgcgtc acatgggcct tggcccgtat ggactcgtga cgttggccga
     4921 agcgcgcgcc aaggcgcggg acgcgcgtcg acttttgttg gacaaggtcg atcccatcga
     4981 ggcgcgtcgt gcgcagcatc gcaagcgcct acttgaaacc gcgcgaggca agactttccg
     5041 agaatgcgcg gaaggttaca tcgcgtcgca tgaagccggc tggcgcgatc cgaggtcgca
     5101 caagcaatgg gttcggtcac ttgtagctta cgcctatccg cacattggcg atttgtcggt
     5161 taccgacatt gacactgcac tcgtgttggc gacgttggag ccgatctgga aagcaaaacc
     5221 agaaactgca agccgcgtac gcggtcgcat tgaaagcatt ctcgattggg ccaaggcgcg
     5281 cggttatcga gagggcgaga acccggcacg ctggcgcgga catctcgaca atctctt
//
LOCUS       DATLJT010000512         4941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3935760,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000512 DATLJT010000000
VERSION     DATLJT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3935760"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VM910_11875"
     CDS             <1..516
                     /locus_tag="VM910_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63264.1"
                     /translation="LAELNRLKGGDEGNFEPAFKALRELLPNVGAIAANLGAYATLWQ
                     QEQIDIAPYNFNFVQTLKGKDVPVELAIPTSGPVGWATSLHLVANAAEPDLAVKYIDE
                     HLDAGIQAQMLKPPYDVIPTNSKVKLEGAITLAIAKTQDDLAKIRTIDWDKLNPQRGA
                     LIERFNREIKL"
     gene            923..1759
                     /locus_tag="VM910_11880"
     CDS             923..1759
                     /locus_tag="VM910_11880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63265.1"
                     /translation="MTSSPTSLLLPLAALFVAFFVAPLVVLVALSLNADTAMTAWTAA
                     NYLKFFTDRFNYSILWDTLFLGVKATLVCLVFGYPIAWICARASARWQSVLVFLVILP
                     ILTSVVVRTFAWIVILGRQGVLNQVALSLGLAREPLRLLYTEPGVVMVLAQVQMPLMV
                     LPILTVLSKIDPNLADASRVLGAGEWRTLWRVTVPLSLPGVIAGCILTYSACVTAFVT
                     QTLIGGARLIYMPLHIYQQAIGANNWPFASAISVIFMVAVLAAVALLSLIGRKSETYG
                     GA"
     gene            1746..2564
                     /locus_tag="VM910_11885"
     CDS             1746..2564
                     /locus_tag="VM910_11885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63266.1"
                     /translation="MAAPRHSTDWEALSLRMVFGALAAVAIVVLVGPTVIMLLTSFTA
                     SQSLKFPPDGLSLRWYVALLDADQMQAAAWNSLVVAFWTTALSVLLGTAAALGIARSR
                     SPLARACDVLFMSPLLLPALAFGFAALIFIHKLGFAPSIPFLVLGHTVVCVPFVLRTT
                     IAALSQLDPALLDASQSLGASGGMTFRRVTLPLIAPGLGAGAFLAFMASFDNVPVSLF
                     LADERSEMLPIHLWQQIETNLDVRTAAVSGLIVIFTLILMLLAERLAGLTRQMR"
     gene            2577..2933
                     /locus_tag="VM910_11890"
     CDS             2577..2933
                     /locus_tag="VM910_11890"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HVI63267.1"
                     /translation="MPGVSIHVVDVSRGVVAAGMEVELHAVDAGGRLALVAKGRVGAN
                     GLLDAPELSKTFAPGGYVAVFQVVGFYRAQGIALPAVPFLDVVRFHFGISDPAQHYHL
                     PFKCTPWGYSCFRGGA"
     gene            complement(3024..3338)
                     /locus_tag="VM910_11895"
     CDS             complement(3024..3338)
                     /locus_tag="VM910_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63268.1"
                     /translation="MSDEARDQIAAGDVTGMILALIAIVALRYRMRVATLLVWVFVAE
                     TVFDLANATIAGIREELFSTASGVTWLILTFYVPLLWVSLGLIVWQLYSRHREPLSPA
                     RA"
     gene            complement(3627..4091)
                     /locus_tag="VM910_11900"
     CDS             complement(3627..4091)
                     /locus_tag="VM910_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63269.1"
                     /translation="MDGSVSIVDRLQLLTGCTMEVSRHPFYVFDLDEHASLISFVWTE
                     KSVAMTVDDYKDAIREYAGLVLEHRARYALVDLRKSRYRVEDADVLRSWWADEIAPLY
                     NQAGLEKFAFVLPEGEQAPPDETPAKAEAGEKFVTKQFGSKQAAISWLTADS"
     gene            complement(4183..4584)
                     /locus_tag="VM910_11905"
     CDS             complement(4183..4584)
                     /locus_tag="VM910_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63270.1"
                     /translation="MSSTVASNEWGEIIHHEADRILELRWLPSKMTDGAFKATLALYA
                     WQAENIRPSFLLIDATHFRHQFGPGVMQWRDDCIIPRYGAAGVKKFAFHVPAGFPNTI
                     EAGGNETFEGPAIFPTAWFSVRQNALNWLEA"
     gene            4709..>4941
                     /locus_tag="VM910_11910"
     CDS             4709..>4941
                     /locus_tag="VM910_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI63271.1"
                     /translation="MLLKIQALSNQTRFEIVKLVREREMAAGTIAKRFKVTRPAVSQH
                     LTMLREAGLLDERRVGSKRFYVVRPEGFAEIVA"
BASE COUNT          877 a         1560 c         1491 g         1013 t
ORIGIN      
        1 ctcgccgagc tcaaccggct caagggcggc gacgaaggaa atttcgagcc ggccttcaag
       61 gcgctgcgcg agctgttgcc caatgtcggt gcgatcgccg ccaacctcgg cgcctacgcg
      121 acgctgtggc agcaagagca gattgacatc gcgccctata atttcaactt cgtgcagacg
      181 ctcaagggca aggatgttcc ggtcgagctc gcgatcccga caagcggacc cgtcggctgg
      241 gcgaccagcc tgcatctcgt cgccaacgcg gccgagcccg atctcgcagt gaaatatatc
      301 gacgagcatc tcgacgccgg catccaggcg cagatgctca agccgcctta tgatgtgatc
      361 cccaccaact cgaaggtgaa gctcgaaggc gcgatcacgc tggcgattgc caagacccaa
      421 gacgatctcg ccaagatccg caccatcgac tgggacaagc tcaatccgca acggggcgcg
      481 ctgatcgagc gcttcaaccg cgagatcaaa ctctagtctg attcatgaca accgacgcga
      541 ccggcgttcg agtggtcgac gaggcgcgtc gcgatctcga tgcgccccct ctccccgctt
      601 gcggggagag gacgccgcaa gcgccagctc cccacgcgcg caagccgcat gaaccgacgt
      661 gcgccgcaca tttcccgttg actcgcgtaa cgagaatctt ccagaaaagc gtactcacca
      721 acactgcgcc ggccgggatg ggcggaacag cgcttccggc ctctttattg cccgcgcgcg
      781 ggcgtcgccg cgccggcgtc ccgaacgcaa ccctcaggtg caggacgcgg ccgccggcgc
      841 agttccattg ctttcggatt attatttaca atagttggcg caactcacgc gtgtgccgcc
      901 catcgtggcc cagcgggcag cgatgacatc ctcgccaact tctctgctgc tgccgctggc
      961 ggcgctgttt gttgcgttct tcgtggcgcc gctcgtcgtg ctggtcgcgc tctcgctcaa
     1021 tgccgacaca gcgatgacgg catggaccgc cgcgaactat ctcaagttct tcaccgatcg
     1081 cttcaactat tcgatcctgt gggacacgct ctttctcggc gtgaaggcga cattggtctg
     1141 cctcgtcttc ggctatccga ttgcgtggat ttgcgcgcgc gcgagcgcgc gctggcagag
     1201 cgtgctcgtc ttcctcgtca tcctgccgat tctcaccagc gtagtggtgc gcacgttcgc
     1261 atggatcgtg atcctcggcc gccaaggcgt gctcaaccag gtcgcgctct cgctcgggct
     1321 tgctcgggag ccgctgcgcc tgctctacac cgaaccgggc gtggtcatgg ttctggcgca
     1381 ggtgcagatg ccgctgatgg tgctgccgat ccttaccgtg ctgtcgaaaa tcgacccgaa
     1441 tctggcggat gcatcgcggg tgctcggcgc cggagaatgg cgcacgctct ggcgggtgac
     1501 ggtgccgttg tcgctgccgg gcgtgatcgc gggctgcata ttgacctatt ccgcctgcgt
     1561 caccgcgttc gtgacccaaa cgctgatcgg cggggcgcgg ctgatctaca tgcccctgca
     1621 catctatcag caggcgatcg gcgccaacaa ctggccgttc gcgagcgcga tctcggtcat
     1681 cttcatggtc gcggtgcttg ccgcggtcgc gctgctgagc ttgatcggac gcaagagcga
     1741 gacctatggc ggcgcctaga cattccaccg actgggaagc gttgtcgctc cggatggtat
     1801 tcggcgcgct cgccgcggtc gcgatcgtgg ttctcgtggg gccgacggtg atcatgctgc
     1861 tgacgtcgtt caccgcgtcg cagtcgctga aattcccgcc cgacggcctg tcgttgcgct
     1921 ggtacgtcgc gctgctcgac gccgatcaga tgcaggccgc cgcctggaac agtctggtgg
     1981 tggcgttctg gaccaccgca ctctcggtgt tgctcggcac cgccgcggcg ctcggcatcg
     2041 cgcgcagccg ttcgccgctc gcgcgcgcct gcgatgttct gttcatgtcg ccgctgctgc
     2101 tgccagcgct cgccttcggt tttgccgccc tcatcttcat ccacaagctc ggctttgcgc
     2161 cgagcattcc atttctcgtg ctcggtcata cggtggtttg cgtgccgttc gtgctgcgca
     2221 ccaccatcgc cgcgctgtcg cagctcgacc ctgcgctgct cgatgcgtcg caaagcctcg
     2281 gtgccagcgg cgggatgacc ttccgccgcg tcacgctgcc gctgattgcg cccggtctcg
     2341 gcgccggcgc gttcctcgct ttcatggcct cgttcgacaa cgtgccggtc tcgctgtttc
     2401 tcgccgacga gcgcagcgaa atgctgccca tccatctgtg gcaacaaatc gaaaccaacc
     2461 tcgatgtgcg cactgccgcg gtctccggcc tgatcgtgat tttcaccttg atcctgatgc
     2521 tgctcgccga gcgcctcgcc ggcctcacgc ggcagatgcg gtgaggagcg tgagccatgc
     2581 cgggcgtgtc gatccatgtc gtcgatgtgt cgcggggtgt tgtcgcggcg gggatggagg
     2641 tcgaactcca cgcggtcgat gccggcggaa ggctcgcgct tgtcgccaag ggccgcgtcg
     2701 gcgcgaacgg gcttctggat gcgcccgaat tgtcgaagac cttcgcgccc ggcggctacg
     2761 tcgcggtttt tcaagtcgtc ggcttttatc gcgcgcaggg cattgctttg ccggcggtgc
     2821 cgttcctcga tgtcgtccgc ttccacttcg gcatttccga cccggcgcag cactatcacc
     2881 tgccgttcaa atgcacgccg tggggctact cgtgcttccg tggcggcgcc tagttcgggg
     2941 cgaaagcgag tgtgtcgctg cgccggggat cccatgccct gtccgcccac gctgggcgac
     3001 agccgcctcc attccaaatt accttacgcg cgggcaggcg agagcggctc gcggtgtcga
     3061 gaatagagtt gccagacaat gaggccgaga ctcacccaca gcagcggaac gtagaaggtg
     3121 agaatgagcc aagtgacccc ggacgccgtc gagaaaagct cctcgcggat gcctgcgatc
     3181 gtcgcgttcg caagatcgaa cacggtttcc gcgacgaaca cccaaaccag aagagtggcc
     3241 acgcgcatgc gatagcgcag cgcaacaatc gcgatgagag ccaggatcat tccggtgacg
     3301 tctccggccg cgatctgatc cctcgcttca tcggacactg cgaatccgaa tttttgagcg
     3361 gagaagattt ggagcgcaat gtaccgaaag gcttggaccc aaagcagcgc agtaagcgcg
     3421 tcagctcggc tgcgactttt cagccatggt atcacgtacc aagtcgccat taataaaagc
     3481 aagatgaagc tgagtgtcga agcgagcccg gcagctgcgg tgtaaatcat caatgcacct
     3541 attgtgttgc aatggcccag gtcgagattt ccaataaaca agccgtcgcc ttgtcggaac
     3601 tagaccccaa agtttgtgca ttccgttcag gagtcggcag ttagccagga aattgcagct
     3661 tgctttgagc caaattgctt ggtgacgaat ttctcgcctg cttccgcctt ggcgggcgtt
     3721 tcgtcaggcg gcgcttgctc gccttcgggc agcacaaagg cgaacttctc taggccggcc
     3781 tgattgtaca gcggcgcgat ttcgtcagcc caccaagacc taagaacgtc cgcgtcttcg
     3841 acgcggtatc gagattttcg taagtcgacc aacgcgtatc gggctcgatg ctccaggact
     3901 aggccggcat attcacgaat cgcatccttg tagtcgtcga cggtcatggc gaccgatttt
     3961 tccgtccata cgaacgagat gagggaagca tgttcatcaa gatcgaacac ataaaatggg
     4021 tgccgggata cttccatggt gcatcctgtc agaagctgta gacggtccac gatcgagacc
     4081 gagccgtcca cgtacttatc aatccggtcg gtggagttgc gcctggcatg cgtcacttac
     4141 ctgtcgcata tcgagtgatg gctaagctaa gaacaacggt catcacgcct ccagccagtt
     4201 caaagcgttt tggcggacgg aaaaccacgc ggtggggaag atcgcaggcc cttcgaaagt
     4261 ctcgttgccg cctgcctcta tcgtgttggg gaatcccgcg ggtacgtgga aggcgaattt
     4321 tttcacgccg gccgcgccgt agcgcgggat gatgcaatcg tcccgccatt gcatcacgcc
     4381 cgggccgaac tggtgtcgga agtgggtcgc gtcgatcagc aaaaatgacg gacgtatgtt
     4441 ctccgcttgc cacgcataca gcgcgagcgt cgccttgaac gcgccatcgg tcattttgct
     4501 cggcagccag cgaagttcca aaatgcggtc tgcctcgtga tggatgattt ctccccattc
     4561 gttgctggcc accgtggaac tcatcgctca gccctcgctt cgagacccct cgccgcacac
     4621 gattacgcag cgtattgcgc gcggcagcat tttgccgtaa gtttttcctt acgtaaagga
     4681 aaactaacgg agttgacggc aattgtccgt gcttctgaag attcaggcgc tgagcaacca
     4741 gacccggttc gagatcgtga aactcgttcg cgagcgggaa atggcggccg gaacgatcgc
     4801 aaaacggttc aaggtgacgc ggccggccgt ttcccagcac cttacgatgc tgcgcgaggc
     4861 cggtctcctc gacgaacggc gcgtcggctc caagcgcttc tatgtggtgc ggccggaagg
     4921 cttcgccgaa attgttgcgt t
//
LOCUS       DATLJT010000513         4973 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1867884,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000513 DATLJT010000000
VERSION     DATLJT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867884"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1598
                     /locus_tag="VM910_11915"
     CDS             306..1598
                     /locus_tag="VM910_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI63272.1"
                     /translation="MKLTVLNVENWKATATRQEILDRDGLYFIVQPSGVKSWALRYRR
                     KNGGKAVKHTIGSYPAIALKDARSEATRLRAEIERGGDPHNEKVVARRREAQVEDTFE
                     AVARRHIEEYQFRRNRSWEWTARLLGFAVDTEATVAAKECPPLRVVKDGSRDQRGRRR
                     LSLADRWGKRRLGNITDSDVIAVLDSISARTPILANRLHAILHKLFDWARSKKLPVTA
                     NPCVGIERPAREKSRDRVLEDKELRKVWLAADKLGHPYAGIVKLLILTGQRRNEIAGL
                     RWSEVDIDESVLHLPAARTKNGLAHDVPLSAPAQAVISNLPRIVDADLVFTIKRQRVT
                     GFSRAKERLDAASGVTDWTLHDLRRTVASGLQRLGVRLEVTEAVLNHKSGSTAGIVGV
                     YQRHDYAGEKRDALARWGDYVDALVSGTKANGVTLRGR"
     gene            1760..1987
                     /locus_tag="VM910_11920"
     CDS             1760..1987
                     /locus_tag="VM910_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63273.1"
                     /translation="MSDIDSLVPDPKVAEEFHTTLMTLWRWDHDPAKVALGWPPKVKI
                     GARNYRHRSALESFKANLLRRALAKREAASA"
     gene            1984..2166
                     /locus_tag="VM910_11925"
     CDS             1984..2166
                     /locus_tag="VM910_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63274.1"
                     /translation="MTAPTSTMANVFDDDGRYLGAVFARGKFGFEGFDAHENSVGLFP
                     TAKQAANALLLEAGGP"
     gene            2585..4207
                     /locus_tag="VM910_11930"
     CDS             2585..4207
                     /locus_tag="VM910_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63275.1"
                     /translation="MNNKDDLALHPLVRQMIHEGMRDKPLTLNWAPLERDAYNCLGLR
                     DLRSTLAIKARSQIITEVLVGGNSFISYSRRTNYYTEEQRYYRPTYSYRAIVPAVDQL
                     ATEGLIEHEKMPQGHRGLQSRFRAAPELIKELAPVPVQYKPLELIVLRDDTGNPIGYR
                     DNRETRTMRKRLAELNEGLVAQEIGLGDRIIREGDHLDNGGRAQTKLHRVFHRGDFAN
                     GGRFYGGHWQNIPAEGGRDQLTINGQPTVEVDYRGLHIRLLYQEAGKPMPDDPYDIDG
                     WPRDQAKLALLIAINARSHASAVRALADALRGEASIADLFVTADRLLKAAKARHPDIA
                     WALASDAGVRLMRKDSELCERIMLETVRAVGIVPLAVHDSFIVPTAHEGRLRETMEAA
                     ISCDNSTPKIPCDNRGQFRDNLAPRNLSGNLQSDITIWDGGDGWDGEDGLLPLGTGWA
                     WVAVQRTWLDDLTKRLERFKPDYRDGANDPCGECGEATARRTGCQASKGFAVIQLVEM
                     PGTTMTDGRKTNKGPLVKQPVAMSDTRVTRLP"
     gene            4861..>4973
                     /locus_tag="VM910_11935"
     CDS             4861..>4973
                     /locus_tag="VM910_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI63276.1"
                     /translation="MPLYGYARVSTRDQDLASQDAELMAAGCAKVFKEKISG"
BASE COUNT         1099 a         1438 c         1520 g          916 t
ORIGIN      
        1 agacgcggcg ctggaacctt cacaaatatc ttaccggccg cggcggcaac atcgccgcgg
       61 atcgacgccc gcatcgtcgc tgcgatcatg aaggagccac cgccgctgga ataaaacgcc
      121 gctgcgagcg ttgacaagct gccacgtatc cagcgaagat aagcctttcc tatcaacgct
      181 ccccgttaac ggccgtttgc agtagtcctt gtcttgcagg ggttagttac gttgcgttat
      241 cctctctggg taacgagacc taacaggggt atttgctacc cctgacgcta ccccgggggc
      301 acgtgatgaa gctaactgta ctcaacgtcg agaactggaa agcgaccgcg acgcgccagg
      361 agattctcga tcgcgatggt ctctacttca tcgtccagcc gagcggagtg aagtcatggg
      421 cgctacgcta ccgccgcaag aacggtggca aggcggtcaa gcacaccatt ggcagctatc
      481 ccgcgatcgc gctcaaggac gcacgctcgg aagccactag gctgcgcgcc gagattgagc
      541 gcggtggcga tccgcacaat gagaaggtgg tggcgcgacg ccgcgaggct caggtcgaag
      601 ataccttcga ggcagtcgct cggcgccaca tcgaggaata tcagtttcgt cgtaaccgta
      661 gctgggagtg gaccgcgcga ttgcttggtt tcgcggtgga caccgaggcc acggtcgcgg
      721 cgaaggaatg tcccccactg cgggttgtga aggacggtag cagggatcag cgcgggcgcc
      781 gccgtctttc gctcgctgac cgatggggca agcgccgtct tggcaatatc accgactccg
      841 acgtcattgc cgttctcgac tcgatcagcg cgcgcacgcc gatcctggca aaccggctgc
      901 atgccattct ccacaagctg ttcgactggg cacggagcaa aaagctcccc gtcaccgcca
      961 acccgtgtgt cggaatcgag cgcccggcca gggagaaatc ccgcgatcgc gtgctggagg
     1021 acaaggagct gcgcaaggtg tggctggcgg ccgacaagct tggccatccc tatgccggca
     1081 tcgtcaagct attgatcctc accgggcagc gccgtaacga gatcgccggc ctgcgctggt
     1141 ccgaggtcga tatagacgag tccgtgctgc atctgccggc ggctcgcacc aagaacggct
     1201 tggcgcacga cgtgccgcta tccgcgcccg cgcaggctgt tatttccaac ctgccgcgca
     1261 tcgtggacgc cgacctggta ttcacgatca agcgccagcg agtgaccggg ttctcgcggg
     1321 cgaaggaacg gctcgacgcg gcaagcggcg tgaccgactg gacgcttcac gaccttcgca
     1381 gaacggtggc gagcggcttg cagcggcttg gcgtgcgcct tgaggtcacc gaggcggtac
     1441 tgaaccacaa gagcggcagc acggcgggca ttgtcggcgt ctatcagcgg cacgactacg
     1501 ccggggagaa gcgcgatgcg ctggcgcggt ggggcgatta cgttgatgcc ctggtgagcg
     1561 ggacgaaggc caacggtgtc acgttgcggg ggcggtgagc cgcgctccca acgggcctca
     1621 aaattgccta ctttggtgct gcaatagatc ggctccgcaa ccaacggagc ctttttttat
     1681 ttcgcgtgcg cttttttctt tgcgatcctc gcgaatcggg tcttataagc ctggctccga
     1741 aaccaacgga gcctttgtta tgagtgacat cgacagcctc gtgcccgacc cgaaggtcgc
     1801 cgaggaattt cacaccacat tgatgacgct atggcgctgg gatcacgatc cggcaaaggt
     1861 cgcgctcggc tggcccccga aggtcaagat cggcgcccgc aattatcgcc accgcagtgc
     1921 gcttgagtcg ttcaaggcca atctgctgcg gcgggcgctt gccaagcgtg aggcggcgag
     1981 cgcatgaccg ctcccacctc gacgatggcc aacgtctttg acgacgacgg ccgatacctc
     2041 ggcgcggtct ttgctagagg caagttcgga ttcgagggtt ttgacgccca cgaaaatagc
     2101 gtcggcttat ttccaactgc gaagcaggcc gcgaacgcgc tcttactaga ggcgggcggc
     2161 ccatgagcgc cacctgccac gcttgcggct cgcagtttag gccacagcgc tcgacggcgc
     2221 gcttctgtag cccgcgttgc cggaaggcga cgcagcgagc ccgcgaccga ggaatgccta
     2281 ttaacgtggc cgcgacacgc cctggcgtgg gcctggacgc ggttcttagc gtgacagcga
     2341 ctatcgggat gtccgagggc caaaaaccgc aaagtgtcac gctaaagcgc aaacccgcca
     2401 aactcgaccc gcgcatcgtc cccgatccga agtggccggg aatgtatcgg attaggcgac
     2461 cagatggctc gctttctgag atggcgaatt tgactcgctg tcgtgatgcg cttcgcagtt
     2521 tcgatgaggc ccaataagac gctcacggcg gaccaaggcc gagatcgacg ctatcaagaa
     2581 gcccatgaac aacaaagatg acctagcgct acacccattg gtgcggcaga tgatccatga
     2641 aggtatgcgc gacaagccgc tgacattgaa ctgggcaccg ctagagcgcg acgcctataa
     2701 ttgcctcggc ttacgtgatc tgagaagcac gctcgccatc aaggcgcggt cacagatcat
     2761 cacagaggtg ctggtcggcg gcaatagttt catctcctat tcgcgcagaa cgaactacta
     2821 caccgaagag caacgctatt accgccctac ctatagttat cgcgctatag tccccgccgt
     2881 ggatcagttg gcgaccgaag gattgattga acatgaaaaa atgccgcaag ggcatcgcgg
     2941 cctacaatcc cgcttccgtg ccgcaccgga attgatcaaa gaactcgcgc cggttccggt
     3001 gcaatataag ccgctcgaac tgatcgtgct tcgagacgac actggcaatc ctatcgggta
     3061 ccgcgataac cgcgaaaccc ggacgatgcg taaacgcctg gcagaattga acgaaggatt
     3121 agtggctcaa gagatcgggc tcggcgaccg gataatccga gagggcgacc atctcgataa
     3181 tggcggtcgg gcgcagacca aactgcatcg cgtctttcat cgcggcgatt tcgctaatgg
     3241 cggccggttc tacggcgggc attggcagaa cattcccgcc gagggcgggc gcgaccagct
     3301 cactatcaat gggcagccga cggtggaggt agactatcgc ggcttgcata ttcggcttct
     3361 ctaccaagag gccggcaagc caatgccgga cgatccttat gatatcgatg gctggccacg
     3421 agatcaggcc aagcttgcgt tgctgatcgc gatcaacgcc cgaagccatg ccagcgccgt
     3481 ccgggcgctt gctgatgcac tgcgcggcga ggctagtatt gctgatctct ttgttacagc
     3541 ggatcggctg ctaaaggccg cgaaggctcg gcacccggat attgcatggg cgctcgcatc
     3601 cgacgcaggc gttcgcctga tgcggaaaga ttccgaactg tgcgagcgca tcatgctgga
     3661 aacagtccgc gcagtcggca tcgtgccgct cgccgtgcac gactctttca tcgttccaac
     3721 ggctcatgag ggcagactga gagagacaat ggaagcagca atctcttgtg ataacagcac
     3781 gccaaaaatc ccttgtgata acaggggtca atttcgcgac aacttagctc ccagaaatct
     3841 ttcgggaaac cttcaaagcg atatcacaat atgggacgga ggggacgggt gggacggaga
     3901 ggacggcctc ctgcctcttg ggacggggtg ggcttgggtt gcggtacagc gaacgtggct
     3961 cgacgatctg acgaaacggt tggagcgctt caaaccggat taccgggacg gagctaatga
     4021 tccatgtggc gaatgtggtg aagcaacagc tcgacgaact ggatgccagg cgagcaaagg
     4081 cttcgcagtg atacagctcg tggaaatgcc gggaaccacg atgactgatg gccggaaaac
     4141 caacaaaggc cctctcgtga aacaaccagt agctatgagt gacacgcggg tgacacgact
     4201 tccataaagg gacgtaggga cgccatacgt cggatagtca aaatcgcgtc actcgcgtca
     4261 ctgggcagag gcttgatgca ggctcgaacg tgcgcagcat ctgtggacga gtagaaaaaa
     4321 tgaggcggaa gaactgcacg ctttggggtg gcgcacggcc tcgccctatc accggcagaa
     4381 gttgaaccaa gactcgagga gtaagcctgt gaccccacaa cgttgttctc caaccactac
     4441 cctcgccctc acccgagacc caatcaggta agagcacact ccctccacag ggggaataac
     4501 agaaggagag gcagaatgtt gcataacagc cacaccacaa gatgagcaac atcaaagacg
     4561 taaagctctt ttggaggggg tttgaagtag ccacgggctc cgcggggtcg gtagctgaca
     4621 gctccatcgc gcgcgcatct cttaaaaatc ggcgagcgcc caaatcctca gtcggaacgc
     4681 gttccggctc cggattaaac caggcttgcc ggcgtgcgct tgagagcatc ggcgccgtcg
     4741 ggggagaact gcaaaaatta aaagttttgc atgtttttgt tgacggccaa aattgggcag
     4801 attattcctg catatgttag gcgcgaaagt ggaagtgtcc tatcatggag ggtaaaattc
     4861 atgccgctgt acggatatgc gcgggtttcg acccgcgatc aggacttggc ctctcaggac
     4921 gccgagctga tggccgccgg ttgcgccaaa gtgttcaagg agaagatatc ggg
//
LOCUS       DATLJT010000514         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4166304,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000514 DATLJT010000000
VERSION     DATLJT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4166304"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VM910_11940"
     CDS             complement(<1..218)
                     /locus_tag="VM910_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63277.1"
                     /translation="MIAAMIRREFITLLGGAVAWPLAARAQPGEHMRRIGVLIALAED
                     DPEAKALVAGFLQGLAKRGWSKDRNLHI"
     gene            complement(215..1114)
                     /locus_tag="VM910_11945"
     CDS             complement(215..1114)
                     /locus_tag="VM910_11945"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63278.1"
                     /translation="MPVIGYLSALSESQIPNLLEALRRGLSEGGFVEGMSVIIDYRFA
                     EGEYGRLQTFATEFVRRPVSLIVAVGPPAAFAAKVATTSIPIVFSVGLDPVAAGLVTS
                     FNHPGGNATGVSLITGPLGQKRLELLRELAPKASTIAMLINPVSPDAVPEIRDVEQAA
                     QANGLGLKILNASTPAEIERAFAEIAESKPDALLVGSDPFYVLHRKQITSLVAPLRIP
                     AIYPFREFLAAGGLLSYGASLVGAQRQVGIYAGRILRGAKVADLPVLQPTTFELVLNL
                     KTASAQGIEVPPMLHARTDEVIE"
     gene            complement(1189..1908)
                     /locus_tag="VM910_11950"
     CDS             complement(1189..1908)
                     /locus_tag="VM910_11950"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63279.1"
                     /translation="MTPDVILADGGSVVGLLLQEIRSVPIVFTLTPDPVGAGFVESLA
                     RPGGNATGFTLFEYGIGGKWLELLKEITPGVMRAAVVRDPALPQGTGLFGAIQSAASS
                     FGVELTPVNVRDASEIERAIAAFARSPHGGLIVTASILAVLHRDLLVTLAAQQKLPAV
                     YFHRLFVAAGGLISYGPDLSEQYVRAAGYVDRILKGEKPADLPVQAPTKYELVINQKT
                     AKALDLAVPDSLLARADEVIE"
     gene            complement(1905..2177)
                     /locus_tag="VM910_11955"
     CDS             complement(1905..2177)
                     /locus_tag="VM910_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63280.1"
                     /translation="MKRREFITLLGGATVGWPLAARAQQAERMRRIGVLSPATETDPT
                     DRRLLEVFRQALQKLGWIDGRTVLIDTRWTAGDPDRARKYAANWSR"
     gene            complement(2174..>2566)
                     /locus_tag="VM910_11960"
     CDS             complement(2174..>2566)
                     /locus_tag="VM910_11960"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63281.1"
                     /translation="LNASTDAELDAALAALVQQRVLAMLVVGDPFFDTRRDHIIAFAA
                     QQKLPALYQFREYAMDGGLMSYGVSFPEAYREVGIYAARILNGAKPEDLPVMQSVKFE
                     LVINLKTAKVLGLQIPDKLLALADEVIE"
BASE COUNT          522 a          782 c          758 g          504 t
ORIGIN      
        1 tcgatgtgaa gattgcggtc ctttgaccat ccccgctttg caagtccttg caggaatccg
       61 gcgaccaacg ctttcgcttc cggatcgtcc tcggcgaggg cgattagcac gccgatccgc
      121 cgcatgtgtt cgcccggctg cgcgcgcgca gcgagcggcc acgcaaccgc gccgccgagc
      181 agcgtgatga actcgcggcg gatcatcgct gcaatcattc aatcacctcg tcggtgcgcg
      241 cgtgcaacat cgggggcacc tcgatgccct gcgcgcttgc agtcttaaga ttgaggacca
      301 attcgaacgt ggtcggctgg agcaccggca aatccgcaac tttggcgccc ctgagaatac
      361 gcccagcata aatcccgact tgccgttggg cgccaactag actcgcgcca tagctgagta
      421 atccaccggc ggccaggaac tcacgaaagg ggtagatcgc aggtatccgc agaggcgcta
      481 ccagtgacgt tatctgcttg cggtgcaata catagaacgg gtccgaaccg accaacaatg
      541 catcaggctt gctttccgca atctcggcga atgcgcgttc gatttcagca ggcgtactcg
      601 cattaagtat tttgagcccc aaaccgttcg cctgtgccgc ctgctctaca tcccggatct
      661 ctggcacagc gtccggactg acaggattga tcaacatggc gatcgtggat gccttaggcg
      721 cgagttcccg caggagttcg agccgcttct gtccgagcgg tccagtaata agggaaacgc
      781 ctgtagcgtt gccgcccgga tgattgaagc tcgtaactag gccagccgcc accggatcga
      841 gtccgacgga gaagacgatc ggaatcgagg ttgttgcgac cttggcagca aacgccgctg
      901 gtggaccaac tgcaacgata aggctaaccg gccggcggac gaactcggtc gcgaaggttt
      961 gtaagcgtcc gtattctcct tccgcaaacc gatagtcgat gatgacgctc ataccctcca
     1021 cgaatccacc ttcgctcaag ccccgccgta gtgcctcgag cagatttggg atctgcgatt
     1081 ccgagagcgc gctgagatag ccgattaccg gcatcgattg ctgctgcgcg cttgccgcga
     1141 gcggccacgc cgccgcgccg ccgagcagcg ctacgaactc gcgccttctc actcgatcac
     1201 ctcgtcggcg cgggcgagca ggctatcagg cacggcgaga tcgagtgcct tggcggtctt
     1261 ctgattgatg acaagctcgt atttggtcgg tgcctgcacc gggaggtcgg ctggcttctc
     1321 gcctttgaga atgcggtcaa cgtagcccgc cgcgcgcacg tactgttcgc ttaagtcggg
     1381 tccataggag atcaggccgc cagcggccac aaagagtcga tggaagtaaa ccgcaggcag
     1441 tttttgctgg gcagcaagcg tgacgagcag atcacgatgc agtacggcca atatgcttgc
     1501 cgtgacaatc aagcctccgt gaggcgagcg cgcgaatgcc gctatggcac gctcgatttc
     1561 actagcatcg cgcacattta ctggagttaa ttccactccg aaagacgagg ccgccgattg
     1621 gattgcgccg aacaaaccag tcccttgagg tagggcggga tcgcgaacga cggccgctcg
     1681 cattacgccg ggggtgatct ccttgagcaa ttctagccat ttcccgccga tgccgtattc
     1741 aaaaagggta aaaccagtgg cgttgccgcc cggtcgcgcc aaactttcga cgaagcctgc
     1801 accgacggga tcgggcgtta acgtgaacac gatcggcacg ctgcgtattt cctgtagcaa
     1861 cagccctaca actgagccgc catcggcgag aatgacatcg ggtgtcaacg cgaccagttc
     1921 gccgcgtatt tgcgggcgcg gtcgggatca cccgcagtcc agcgggtgtc gatcaacacg
     1981 gtgcggccgt caatccagcc taatttttgt agagcctgtc ggaaaacctc aaggagacgc
     2041 ctatcagtcg ggtcggtctc cgtggcaggg ctaagcacgc cgatgcgccg catccgctcg
     2101 gcctgttgtg cacgtgccgc cagcggccac cccaccgttg cgccgccgag cagcgtgatg
     2161 aactcgcggc gtttcactcg atcacctcgt cggcgagtgc cagcagtttg tctggaattt
     2221 ggaggccgag cactttggcg gtcttgaggt tgatcaccag ctcgaattta accgattgca
     2281 tcaccggaag gtcttcgggc ttcgctccat tgagaatcct cgccgcatag atgccgactt
     2341 cgcgataagc ttcaggaaag ctcacgccat aactcatcaa tccaccgtcc attgcatatt
     2401 cgcgaaattg gtagagggcg ggcagcttct gttgtgccgc gaaggcaata atgtgatcgc
     2461 gccgggtgtc gaagaacgga tcgcccacca ctagcatcgc taggacacgc tgctgcacca
     2521 gagcagccag ggcggcgtcg agctcggcgt cggtgctggc gttcag
//
LOCUS       DATLJT010000515         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1179514,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000515 DATLJT010000000
VERSION     DATLJT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179514"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VM910_11965"
     CDS             complement(<1..1224)
                     /locus_tag="VM910_11965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVI63282.1"
                     /translation="MLKSLIVGIVDLCTQHPWWLIGPAAVLAAGSGVYAGTHFAIRTD
                     TKMLISADLPWRQREERFLESFPQRQILVVIDAPTPELVEQAAAKLAHTLATRSELIH
                     SVRQIQGGNFFARNGLLYLPTDEVARVTDGLTRANVLLKTLAADPSLRGILHALSLGI
                     TGVQRGALKLDDMTRPMTMAAATAEDVVAGRPASFSWRALAGGKPAEPTDLRRFIEVE
                     PMLDFMALEPGRAATDAIAQTASDLKLGADYQARVRQTGRIPTDDDEFRTLKENAGLN
                     AAVSLLAVLIILWLALRSARIIFAVAVSLLVGLAISAAWGLFLVGALNLISIAFFVLF
                     IGLGVDFGIQFSLRYRAERHDYPVLRTALHSTAVKVGGPLALAAFATAVGFSSFLPTA
                     YRGLSELGEIAGSGMI"
     gene            1745..>2211
                     /locus_tag="VM910_11970"
     CDS             1745..>2211
                     /locus_tag="VM910_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63283.1"
                     /translation="MRRRDIIKVVAGSAIAWPLAARAQQPTIPVVAFLRTGSADIESR
                     NVAAFRIGLNETGYVEGQNLTVEYHWLESPYDRLPALIADLVRRQVAVIATVGTPLVA
                     LAVKAATATIPIVFGEGIDRSSLALSPASLTPAATRPAPIFWQQRLRLSGCGS"
BASE COUNT          448 a          670 c          687 g          406 t
ORIGIN      
        1 gatcatcccc gagcctgcaa tctctccgag ctcggagagc ccgcggtagg cggtaggtag
       61 gaacgaggag aatcccaccg cggtcgcgaa cgcggccaag gctaaaggtc cgccgacctt
      121 gaccgccgtg ctgtgcaagg ctgtgcgcag cacgggataa tcgtgacgct cggcacggta
      181 ccgtaggctg aactgaattc cgaaatcgac gcctagcccg ataaacagca cgaagaaagc
      241 aatggagatt aggttgagcg cgccgactag gaacaggccc caggcggcgg aaattgccag
      301 ccctactagg agactgacgg ccaccgcgaa gataatccgc gccgagcgca gtgcgagcca
      361 taggatgatc agaaccgcaa gcagcgatac ggcggcattg aggcctgcgt tttccttgag
      421 ggtcctgaac tcgtcgtcgt cggtcgggat acgcccggtt tgacgcaccc gcgcctgata
      481 atcggcgccg agtttgagat ccgaagcggt ctgcgcgatc gcatccgttg ccgcgcgacc
      541 aggttcgagc gccatgaagt cgagcatagg ctcaacctca atgaagcggc gcaggtccgt
      601 cggctccgcc ggtttgccgc cggccagcgc gcgccacgag aagcttgcgg gccggccggc
      661 aacaacatct tccgctgtag cggcggccat ggtcatcggt ctggtcatgt cgtcgagctt
      721 cagcgcgccg cgttgcaccc ccgtgatccc aagcgacagc gcgtggagta ttccccgcag
      781 gctcggatcg gccgccaagg ttttcaggag gacatttgct cgcgtcaacc cgtccgtcac
      841 ccgtgcgacc tcgtcggtcg gcaagtacaa caagccgttc cgcgcaaaaa agttgccacc
      901 ttggatttga cgaacggaat gaatgagttc ggaacgcgtt gcgagagtgt gggccagttt
      961 ggcagccgct tgctcgacga gctcgggcgt cggcgcgtca attactacga ggatctgccg
     1021 ttgcgggaac gactcgagga atcgctcctc ccgctggcgc cagggcagat cggccgagat
     1081 aagcatttta gtgtcggttc ggatggcaaa atgcgtcccg gcgtagacac ctgaacctgc
     1141 cgccaaaacc gcagccggcc cgatcagcca ccatggatgc tgggtgcaga gatcgacgat
     1201 accgacgatg agcgatttca gcatgtgttg ccactcaccg agcggattag cagtgggccg
     1261 cgtataggca tgggcagcga gctggcctgg cgaacggcca agcccgcaat ccctgaaaga
     1321 cggtggcctc cttacccggg aacccggcgt cgatgagtgt cgatccgtcg tcgcactcga
     1381 tcagaaaggc gtttgccatc cgcatcggga cgacatgcac gccgttgatc acacgcttcg
     1441 cgcccacgga tggagctcct tacaccggtg taaacgcgca aggcggcttg tctgtttctg
     1501 gcgcttagcg gacatgctca cgacacccag cacgtaatcg agtgaagcgg caggatgtcc
     1561 gcttttggca caaagcagac atcctaatgg gcttagccga tgtttgcttt tgggggtaaa
     1621 gcggacattg aatttgaggg gcgtcgcagg attgctgccg tgcaaactgc ccctaaaccc
     1681 catttcactg gacgcaaatc cctgatgtaa tcggccccgg cgtgcttgag ctgtgggggc
     1741 tgtaatgcga cgccgcgata tcatcaaagt cgttgctggt tcggccatcg catggccgct
     1801 cgcggcgcgt gcgcaacaac cgacgatccc ggttgtcgcg tttctccgga ccggatcggc
     1861 cgatatcgag tcacgcaatg tggcggcgtt ccgcataggc ctcaacgaga ctggttacgt
     1921 cgaaggccag aacttaacgg tcgagtacca ctggctggaa agtccatacg atcgtctgcc
     1981 ggcactgata gccgacttgg tccgccgcca ggtggccgtg atcgccacgg taggcacccc
     2041 gctggttgcg ctcgcggtga aagctgcaac agcaacgatc ccaatcgtct tcggcgaagg
     2101 tatagaccgg tccagcttgg ccttgtcgcc agcctcgctc accccggcgg caacgcgacc
     2161 ggcacctatt ttttggcaac agagattacg gctaagcggc tgcggctcct g
//
LOCUS       DATLJT010000516         2460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1868016,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000516 DATLJT010000000
VERSION     DATLJT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868016"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..818)
                     /locus_tag="VM910_11975"
     CDS             complement(333..818)
                     /locus_tag="VM910_11975"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI63284.1"
                     /translation="MSSAEEQDRLAWKRERDLERTAQCHCGALKVITSGEPVQVYLCH
                     CQSCQRRTGSVAHCGSRWLKSQVRIEGDHKIYTRKGDSGFSPRFHFCPNCGSNVFIDG
                     DRAPDLCIITVGSFADPNFPAPTASIYEGSRHSWFGLSTVSHHFAQGSPRDARGFPIN
                     H"
     gene            1104..1433
                     /locus_tag="VM910_11980"
     CDS             1104..1433
                     /locus_tag="VM910_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63285.1"
                     /translation="MSLPVHLVGSIGLDTVDDVFAACGELLGPYLKRVPDGEPGGRRL
                     WISWQFPLLRASPFLKADVSRHRAGGVGFHPLMVAEGVNPEDVRFGELGYAREARLLS
                     RFRCSAR"
     gene            1438..2130
                     /locus_tag="VM910_11985"
     CDS             1438..2130
                     /locus_tag="VM910_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63286.1"
                     /translation="MLPSNVRFQVCLPTPFAVVAPFVAENSRDAVLRAYEAAMLREVA
                     AVCAGIPHHDLALQWDVCIEMVMWDGRWRGGPPFPGMEKVFADTFARLAAAVSGNVEL
                     GFHLCYGDLDARHFVEPVDMTKMVELANLIAASAQRPIAWMHMPVPIARDDDAFFRPL
                     RKLHLDAGTELYLGLVHAEDGLAGAQRRIAAARKFAGRSFGVASECGIARGRTPARVR
                     QFLEVYAAAAAG"
BASE COUNT          462 a          755 c          752 g          491 t
ORIGIN      
        1 acattcgaga gcttacgatc ccacccgccg agatcggata taggcagcgc acgcgttcag
       61 cggtgtttca gcggcaaccc ttgaagtggt cgagttctca gtgcccgtgt tcgccagtgt
      121 agtagtcatt gccgcgtgag aattcgacaa acacgcaagg ctcgctgcct accgaccacg
      181 catcgtgacc cggtggcagc aacatcacat cgttttttga gaagtcttgc tgcgagccat
      241 cgtccatgac gacccggagc gttccagaga gcactagtgg tacaaaccat acgcttggta
      301 cccgcctcgg aagattttcg agcatgccga gtttaatggt ttattgggaa gcctcgggcg
      361 tcacgcgggg aaccctgcgc aaagtgatgg gagactgtcg acaggccgaa ccaggaatgc
      421 ctcgacccct cgtatattga tgcagtcggc gcaggaaaat taggatctgc aaaagagcct
      481 actgtaatta tgcaaaggtc cggtgcccga tcaccgtcta taaagacatt gctcccgcaa
      541 ttcgggcaga aatgaaagcg aggtgaaaat ccactatcgc ctttgcgagt gtaaatctta
      601 tggtctcctt caatccggac ctgggatttt agccagcgtg agccgcaatg cgccacagag
      661 ccggtacggc gttggcacga ttgacaatgg cacagataga cctgcacggg ctcgcccgac
      721 gttatcactt ttaaagcgcc gcaatggcac tgcgcagttc gttccaaatc cctctccctt
      781 ttccatgcca acctatcttg ctcctccgcg ctggacaccg tcgagcgttt atcggtaggg
      841 gcttaaatgt tagattttga ccactagctt gggctgggcc gtgaattcac aagcggcctt
      901 taccctggcc tgagtagaga ggtccgcttc ccatgtaagg cggactcccc gacagtgagg
      961 ggccggtcag gggcaaagac gggttcccat caacttgcct gagcgtccgc tctcaaccgc
     1021 agtccagtcg tcgctaccgg cgttgacaac aatgcccgcc gagcgatcct cggccgcaaa
     1081 cgcattgaac acggagggtt gctatgtcgc tccctgtcca tctggtcggc agcatcggcc
     1141 tcgatacggt tgacgacgtg ttcgccgcct gcggcgagtt gctcggaccg tatttgaagc
     1201 gcgttccaga cggcgagccg ggtggccggc gattatggat cagttggcag ttcccgctgc
     1261 tgcgcgccag cccgttcttg aaggcggatg tctcgaggca tcgagccggc ggggttggct
     1321 ttcatccgct catggtcgcc gagggggtta accccgagga cgtgcgtttc ggcgaactcg
     1381 gatacgcccg cgaagcgcgc ctcctatctc gatttcgttg cagcgcgcga taatggcgtg
     1441 ctgccatcga acgtgcgctt tcaggtgtgc ctgccgacgc cgtttgccgt cgttgcgccg
     1501 tttgttgccg aaaactcgcg cgatgcggtc ctgcgggcgt atgaggcggc gatgctgcgc
     1561 gaggtcgccg ccgtgtgtgc cggcatcccg caccacgacc tggcgctgca atgggatgtc
     1621 tgtatcgaga tggttatgtg ggacggcaga tggcgcggcg ggccgccgtt ccctggcatg
     1681 gagaaggtct tcgccgatac cttcgcgcgg cttgctgcgg cggtgtccgg caatgtcgaa
     1741 ctcggctttc atctctgcta cggcgatctc gacgcacgcc acttcgtcga gccggtcgac
     1801 atgacgaaga tggtcgagtt ggcgaacttg atcgccgcga gcgcccagcg gccgatcgcc
     1861 tggatgcaca tgccggtgcc gatagcccgc gacgatgacg cgttcttccg gcccctgcgc
     1921 aagctgcacc tcgacgccgg cactgaactt tatctgggtc tcgtgcatgc ggaggacggc
     1981 cttgcgggcg cacagcgccg gattgctgcc gctcgcaagt tcgccgggcg gtcgttcggt
     2041 gtcgcctccg aatgcggcat agcacgtggc cgaacgccgg cgcgcgtcag gcaatttttg
     2101 gaggtctacg ccgccgccgc ggccggatga ttatgcggat cgggccgacg gccgagatga
     2161 ccgcgaaatc acatggccgc acgaccggta aacctgcgtg cggcccggcg tagagccgac
     2221 tttcgcgagc ggaatgggag ggcccagggt gcgattagtc cgggcaccgg tagcgggtca
     2281 actaagctac caacggctac cccaaccgcc ccaaccgcac cgccacctcc agccgcgtcc
     2341 acctgaaaat cctgctcagc caacgcgtca acgcggcgct caaaggaagc atcgaaaccg
     2401 agcagctcgg cgcgctcacc ctgaggggcc tgacccagcc ggtgattgcc ttcaacgtcc
//
LOCUS       DATLJT010000517         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_489984,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000517 DATLJT010000000
VERSION     DATLJT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489984"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..969
                     /locus_tag="VM910_11990"
     CDS             160..969
                     /locus_tag="VM910_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI63287.1"
                     /translation="MTARQQRRLALLVVLMLMSAVVASTKVQSQLAPYGRDPPNVFDG
                     ILSNVAGSSEISTAQLRAALNDGSVIILDARPYEEYAVSHIPGARAVPAKAGTTPALY
                     VGDATEVAKAIPDQTQPLILYCNGLFCGRSERLADDLIKLGYRNVRRYQLGAPGWRAL
                     GGVMQVEKPALLRLLAQDATSVLIDGRAKAGLKPRLHNAVSIPLPEASQAKDDGRLPM
                     TDHNTRIFVVGTNGAEARAVAEAIVHDAFHNVTFFGGAITDLSELLEDEEG"
     gene            1169..2101
                     /locus_tag="VM910_11995"
     CDS             1169..2101
                     /locus_tag="VM910_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63288.1"
                     /translation="MRRLIVVMAILGVAHSAHAHGIAGNRFFPGTLSFDDPAVADESI
                     LPLFSSSKHPDEGGDVVDKRFSWSIFRLLTPTLGVGVDSGWVHRDWSSARSSGFDTTN
                     LTVKGEIYRNDLHETLVSGGLGWGIGHSGAQGAGANAPDLIAPGIFFGKGFGDLPANL
                     AWLRPFGITDALTFEHPMRGTSTNFGVDSMTGQLGPMLTRNVDTLHWGFALEFSTLYL
                     TDRFTPGKLPKEEPLNQFVPLVEFSFVSPRGEKTVATMNPGLSYVAVSWQVAAEAIVP
                     LNSEAGRSIGARAQLLLFLDELIPSLFGKPLLTH"
     gene            complement(2210..3187)
                     /locus_tag="VM910_12000"
     CDS             complement(2210..3187)
                     /locus_tag="VM910_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI63289.1"
                     /translation="MTIPAVGGYRLVQTIAIAGDDGWDHPTVDSAARRLYVTHGTHVV
                     VIDVDSGKLVGKIDDTPGVHFTVIDPELDRGFISNGGAARLTIFNTNTLETIGQVKST
                     GENPGPTVFDPATKRVFTFNLNSHNATVVDSEEGNVVGAFDLGGRPELVGTDAKGNVF
                     ANLVQNNVVLQIDARKMTPGQTWPVAPGVGPRTMAVDQKNGRLFIGCANRRMVILDSN
                     NGRVIGSLPIGPGPDDSAYDPQTRLVYTSNGGDGAVSIIQQDSPDDYRVFETVKTAPG
                     ARNMALDLKTKKIFLPLSDREPTPPPSGEAPSPRGKIMPGTFRVLVFGM"
BASE COUNT          657 a          916 c          954 g          746 t
ORIGIN      
        1 cgcctctcgc acggtctgcg ccgcgcactt gataaaagtg tcttaaacag actggttcaa
       61 catccgagcc aacaaatgcc caagccgcgg gactcgatga atcctgcgaa tgaaggaagt
      121 cgcgcagatt gtttgcaacg gaggaatagg agctggtcga tgacggcaag gcagcagcgt
      181 agattggcat tgctcgttgt acttatgttg atgagtgcag tagtcgcttc gacgaaggtg
      241 caatcgcaac tcgcaccgta cggtcgtgat ccgccaaacg tcttcgatgg gatcctgagc
      301 aatgtcgcag gtagttccga gatcagcact gcacagctgc gagcggcgtt gaacgacggc
      361 agtgtgatca tcctcgacgc cagaccttac gaagaatatg ccgtcagtca tatccccggc
      421 gcgcgagcgg ttccggcaaa ggcaggcacg acgcctgcgt tgtacgtcgg cgacgcaacc
      481 gaggtcgcga aagccatccc cgaccaaact cagcctctga tcttgtattg caacggcctc
      541 ttttgcggtc gtagcgagcg gcttgccgat gacctgatta aactcggata ccgaaacgtg
      601 cgaagatatc aattgggcgc ccccggctgg cgggccctcg gcggtgttat gcaggtggaa
      661 aagccggcat tgctccggct gctcgcgcag gatgcgacat cagtattgat cgacgggagg
      721 gccaaggcag gtctgaagcc gcgccttcac aacgcagtgt cgataccttt acctgaagcc
      781 tcccaagcca aggatgacgg gcgtctgccg atgaccgatc acaacacccg catctttgtc
      841 gtgggcacca atggcgctga ggcgcgcgcc gtggcagagg cgattgtgca cgacgcgttt
      901 cataatgtta cctttttcgg tggcgcgatt accgatttat ccgagctgct ggaggatgag
      961 gaaggctagc gcgggagtcg gtccgttccg ccggcttttg cgagcatctt gatgattctt
     1021 ccaaatcgcg tcggctcggt tcgagcgcta gatggtggtg gatcgcctcg tgcagattca
     1081 gcatctgttg caacgttgtt acatcctttc agcggaccat atggtagttg acctcgtttc
     1141 cctttgaacg ggccgagaga cgtgttttat gagacggctg atcgtagtga tggccatcct
     1201 cggcgttgct cattcagccc acgcgcatgg cattgcgggc aacaggtttt ttccggggac
     1261 gctttcattt gatgatcctg ccgttgcgga cgagtccata ttgccgctct tctcaagctc
     1321 caagcatcca gatgaagggg gcgatgtcgt cgacaagaga tttagctggt cgatctttcg
     1381 tctactcact ccgacgctag gagttggcgt cgatagcggc tgggttcacc gcgattggag
     1441 cagcgcccgg agttcggggt tcgatacaac caatcttacg gtcaagggcg agatttatcg
     1501 caacgatctg catgagacgc tggtgtctgg gggattgggc tgggggattg gccattcggg
     1561 cgcgcaagga gccggggcca atgcacccga cttgatcgcg ccgggcattt tcttcggcaa
     1621 aggcttcggt gatcttccgg ctaacctcgc gtggctacgg ccgttcggca tcactgacgc
     1681 gcttacattt gaacacccga tgcggggaac ctcgacaaat tttggcgtcg attcgatgac
     1741 tggccagctc ggcccgatgc tcacgcgcaa tgttgacacg ctgcactggg gatttgcgct
     1801 tgaattcagc acgctttatc tgaccgatag attcacgccc ggtaaactgc cgaaagagga
     1861 gccgctaaac cagtttgtac ctctcgttga attttctttt gtctcgccgc gcggagaaaa
     1921 gaccgtggcg accatgaacc ccggcttgtc gtacgtggcg gtgagctggc aagtggcggc
     1981 agaagcgatc gtcccgctca acagcgaagc cggacgttct atcggtgcca gggcgcagct
     2041 gctgctattt ctcgatgagc taattccgtc gctgtttgga aaaccgctcc taactcattg
     2101 atttcacttg aagattgtgt gctttgccta ggcgtaagtt tttgcaattt ctcttcggta
     2161 gcggcactta agtcggcacg ttcaccacac cgcactggcg gtctgcgcct tacatgccaa
     2221 acacaagaac ccggaaggtt ccgggcatga tcttgccgcg ggggctcggc gcttctccgg
     2281 acggcggcgg tgtgggctcg cggtcggata acggcaaaaa tatctttttc gtcttcagat
     2341 cgagcgccat gtttcgcgct ccgggcgcag tcttcacggt ttcgaacacg cggtagtcgt
     2401 ctggagagtc ctgctggatg atcgaaacag cgccgtcgcc gccattggag gtatagacga
     2461 gccgtgtttg cggatcgtag gcggagtcat ccggtcccgg cccgattggt agcgagccaa
     2521 tcacgcgccc attattcgaa tccagaatga ccatgcggcg atttgcgcat ccaatgaaaa
     2581 gccttccgtt cttctggtcc acggccatgg tccgtgggcc cacgcccggc gcgaccggcc
     2641 aagtttgacc tggcgtcatc ttccgggcat cgatctgcag gacgacgttg ttctgcacta
     2701 agttggcaaa aacatttcct tttgcatctg tgccgacaag ttccggtctt ccgcccaggt
     2761 cgaatgcacc gaccacgttg ccttcttcgg agtcgacaac cgttgcgttg tggctgttga
     2821 gattgaaggt aaagactcgc ttcgtcgcag gatcaaagac cgtcggcccc gggttctcac
     2881 ccgttgattt cacctgcccg atggtctcca gcgtattcgt attgaagatc gttagccggg
     2941 cagccccgcc gttgctgata aagccacgat ccagttccgg atcaattacg gtaaagtgaa
     3001 cacccggcgt gtcgtcgatc tttcccacga gttttccgga atcgacatcg atcacgacca
     3061 cgtgtgtgcc gtgcgtcaca tacagtcgcc gggcggcact atctactgtg ggatgatccc
     3121 atccgtcatc gccagcaatg gcaatcgttt gaacgagacg ataaccgcca acggccggga
     3181 tggtcatggc gacccctcct tctcttccgg cggcttgatg tgccgaaacg aaaacaaaag
     3241 cgagaggtcg taggcgatct tcaacacgcc gca
//
LOCUS       DATLJT010000518         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4395611,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000518 DATLJT010000000
VERSION     DATLJT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395611"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1096
                     /locus_tag="VM910_12005"
     CDS             323..1096
                     /locus_tag="VM910_12005"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI63290.1"
                     /translation="MRFQDKVAIVTGGASGMGKEVAIRFVAEGGSVVINGRDVAKAAA
                     AAHEIDPTGKRVAVHIGDVALPATGDAVVKAALDRFGRLDVLFNNAGIFAPKPFLEVD
                     ETEYDRFLDIILKGTFFTAQAAAKAMKAAGHGGAIVQTGSMWALQAIGATPSSAYSAA
                     KAGVHALVKNLAIELAADKIRVNEIAPAVIETPVFNTFLTPEQVAAVLPTFNAFHPLG
                     RHGQVADAAEALLFLASDQASFITGVVLPVDGGVMAGRQ"
     gene            1286..>1577
                     /locus_tag="VM910_12010"
     CDS             1286..>1577
                     /locus_tag="VM910_12010"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
                     /protein_id="HVI63291.1"
                     /translation="MCYQAILQCVQMLKNLESWLDKAEVHAAAKKFDVGVLMAGRLAP
                     DMKPFIYQVQSACDYVKAAAAWLSGQTPPRHEDTEQTIDEVRARIRKTVDFAV"
BASE COUNT          302 a          495 c          487 g          293 t
ORIGIN      
        1 cctgtgggcc catcggagcg agcaccgggc cgtgtggtag cgcctcagcc tggatttccg
       61 ctgtgcaggt cgttagcggc cgttcacagc gacaaatcgg gctgacgcga ttgctccgaa
      121 accagacctc gccacccgca tgcgcgcgcc gccagtcttg acccctccac cgctttttcc
      181 gacaccgcct gacgcgctag cgttacccca ggatctcatt cagagctacc tgctctatcg
      241 atagataggc cttgacggca gcaatttccg aagccattta cggtactaac ctaccgatag
      301 atagccacga aggaaaacgg ccgtgcgttt ccaggataag gttgcaatcg ttaccggagg
      361 cgcttccggg atgggcaaag aggtcgcgat ccggttcgtc gccgagggcg gttcggtcgt
      421 catcaacggc cgcgatgtcg ccaaagccgc ggcggctgcc cacgagatcg atccgacggg
      481 caagcgtgtc gcggtgcata tcggcgatgt cgccttgccg gcgaccggag atgcagttgt
      541 gaaggcggcg ctggaccgtt tcggtcgtct cgatgtgctg ttcaacaatg ccggcatttt
      601 cgcgccgaag cccttcctcg aggtcgatga gaccgaatac gaccgcttcc tcgacataat
      661 tctcaagggc acgttcttca ccgcgcaggc cgccgcaaaa gcgatgaagg ccgccgggca
      721 cggcggcgcg atcgtgcaga ccggctcgat gtgggccctc caggcgatcg gcgcaacgcc
      781 gtcttcggcc tattcggcgg cgaaggccgg cgtccatgct ctcgtcaaga acctcgcgat
      841 cgagctggca gccgacaaaa tccgcgtgaa tgagatcgcg ccggcggtaa tcgagacgcc
      901 ggtgttcaac acgttcctga cgcccgagca ggtggcggcc gtgctgccga ccttcaatgc
      961 attccacccg ctgggccgcc atggccaggt ggcggatgcg gcggaagccc tcttgtttct
     1021 cgcctcggat caggcgagct tcatcaccgg cgtcgtgttg cctgtcgacg gcggcgtcat
     1081 ggctggccgc cagtgaccgc tgacaccaca gtcttatcac cttgggaaat aagcgacacc
     1141 agctccgtcg gacgacggga aaatcgtcgg cgcagcccat ccgagcggcc gtcagcaggt
     1201 tctgccgatt tgtcgttcgg caggcgggat cttccttagg agctctaaca cttcacgatc
     1261 agccaaaacg aaagggaagg actccatgtg ttaccaagcg atcctgcagt gcgtgcagat
     1321 gctgaaaaat ctcgagagtt ggcttgataa ggctgaggtg catgcagccg ccaagaagtt
     1381 tgacgtcggc gtcctcatgg ccggccgcct cgcgcccgac atgaagcctt tcatctacca
     1441 ggtccagagc gcttgcgatt atgtcaaagc cgcggcggcc tggctctcgg gacagacgcc
     1501 gcccaggcac gaggacaccg agcagacgat cgatgaggtg cgcgcccgga tccggaagac
     1561 tgtcgatttc gcggtga
//
LOCUS       DATLJT010000519         5270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1179769,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000519 DATLJT010000000
VERSION     DATLJT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179769"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VM910_12015"
     CDS             complement(<1..759)
                     /locus_tag="VM910_12015"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63292.1"
                     /translation="MRRRAFITLLAGTAVLPVSAFGQQGPANAARIGILTRKTDASVT
                     NQISAFRQGLQDLGWIEGKNVTIEHRDAGGEVIRLAPLAAELVARNVDVIVTVDTPPT
                     QAAEQATSTIPIVIAVSADPVGAGLVKSLSHPGGNATGLSLLAPETDQKTLEVLKETL
                     PGTKRVAMIFDPNNRGMMLRLKAVEVAAAKLSLELQSIAAPGANELALALVAMERPDA
                     VFVLSPIYAAYQKEIAEFATKTGVPLLVDTSGLAG"
     gene            1025..1915
                     /locus_tag="VM910_12020"
     CDS             1025..1915
                     /locus_tag="VM910_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63293.1"
                     /translation="MKAIRTVAILFGLAVMAGSAAAQNAPATSTKKVDGTDNVYIFRY
                     GNHQAMFVVTPQGVIATDPISYQRPAKPYIDAIKAVTDKPIKYVIYSHHHYDHIAGGQ
                     PFKELGATFIAHRRAKERLLELKKQNALLADVVIPDQVVDDKKTIKLGGTTLELIYVG
                     RNHSDNSLVMRLPKEKLVFVVDFAPIESVQFRNIPDNASPLEYIDSLKNLAALDWDRL
                     IPGHPNAGGRLGTKKDVQDDIAYMEDLSSEVKKAADAGKCFDTAMKEVKLPKYEKWAN
                     YDASLPANVERFCYWWGRGY"
     gene            complement(2061..2312)
                     /locus_tag="VM910_12025"
     CDS             complement(2061..2312)
                     /locus_tag="VM910_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63294.1"
                     /translation="MRSWQIISIAVAAVVTAPTVLVALQPPVWLMLLLGPMTLATLYI
                     IARQRSRFDPLDPRFGSCIPPITRPGDKSAVSGRSAPEG"
     gene            complement(2395..2850)
                     /locus_tag="VM910_12030"
     CDS             complement(2395..2850)
                     /locus_tag="VM910_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI63295.1"
                     /translation="MGRIASLKKSIKPSRPSYILDEQIGFILRQVWQRHAAIFARDIG
                     INLTPTQWAALAKLNETGPCSQNQLGRLTAMDVATIKGVIDRLTARGLTETSPDPEDG
                     RRLLVSLTRAGQQLAEKAAPNAVAISKETLAPLDARERETLFALLNKLR"
     gene            complement(2890..3594)
                     /locus_tag="VM910_12035"
     CDS             complement(2890..3594)
                     /locus_tag="VM910_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63296.1"
                     /translation="MKLSVCDLNSFYGPAHILFDIALEVGNGEVVALLGRNGAGKSTT
                     FRSIVGLVEQRSGRIMFDGNDVSRKPTHEIVREGLGYVPEERRIFTELTVEENLEVGR
                     QAKRPNAPHWTREKLFALFPNLGEMRNRPGGRMSGGEQQMLTIARTLMGNPSLVLLDE
                     PSEGLSPKIVEQMVDAILTMKKEGVSIVVSEQNLHFARLISDRAYIIERGRICFGGTM
                     NELDARPDIRDAHLSL"
     gene            complement(3591..4382)
                     /locus_tag="VM910_12040"
     CDS             complement(3591..4382)
                     /locus_tag="VM910_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63297.1"
                     /translation="MNSTSTLLSVKNLTKSYGGVHAVRGVSFELKAGEILALIGPNGA
                     GKSTCFDMLNGQIAPDQGRIALLGEDTTGQKPRDIWRLGVGRTFQITATFPTMTVREN
                     VQVALVSHERRLFNLVASTPKLMREQAGRLLELVGMSGYADRPCGELAYGDLKRLELA
                     IALANNPKLLLMDEPTAGMAPRERIDLMRLTARIAREQTIGILFTEHDMDVVFEHADR
                     ILVLNRGSLIAEGTPEQVRANEEVRAIYLGEGLVYDARHREGASA"
     gene            complement(4379..>5270)
                     /locus_tag="VM910_12045"
     CDS             complement(4379..>5270)
                     /locus_tag="VM910_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63298.1"
                     /translation="ALTVGSEIAIFVIFAASLHFLMSVGGLASFGHAAYFGLGAYGVA
                     FLAKLAGLPMILCLLLGPLLGCLGAAVFGFFAVQLSGVYFAMLTLAFAQIVWSIAFQW
                     VEVTGGDNGILGVWPEKWAASPSHFYWLSLGIAALVVVALRIVVFSPFGFALRATRDS
                     PLRGEAIGIDGKRIQWTAFVIAGTVAGVAGALFAYLKGSVFPDSLGISLSVDALVMVL
                     LGGVETVSGAVIGAIVYKALSIWLVSQTDWSKLVLGAFIVLIVVAFPKGIVGMVESIG
                     RRRGASLKSALFSSRVETAE"
BASE COUNT         1069 a         1624 c         1622 g          955 t
ORIGIN      
        1 gccggcaaga ccgctggtgt cgaccagcaa aggcactccc gtcttcgtag cgaactccgc
       61 gatctccttt tgataggctg catagatcgg agaaagtaca aaaacggcgt caggccgctc
      121 catcgcgacg agcgccagag caagttcgtt tgcgccaggt gcagctattg actggagttc
      181 aagtgaaagc ttcgctgccg caacttcgac ggccttcaac ctgagcatca tgccgcgatt
      241 gttcgggtcg aaaatcatcg ccacacgctt cgttccgggc agcgtctctt tcagaacttc
      301 aagggttttc tgatccgtct caggggcgag cagagacaga cccgtagcat tgccgccggg
      361 atgactgagg ctcttgacca accctgcccc caccggatcg gccgagactg cgatgacgat
      421 gggtatggtg ctggttgcct gctcggcggc ctgtgtaggt ggcgtatcca ccgtgacgat
      481 gacatccaca ttgcgagcga cgagttcggc agcgagcgga gccagacgaa tgacctcacc
      541 gcccgcatcg cggtgctcga tggtgacgtt ctttccttcg atccagccaa ggtcctggag
      601 gccttgtcgg aaggcgctga tttgattcgt aacggaagcg tcggtcttgc gcgtcaggat
      661 gccgatccgg gccgcatttg ctggtccctg ttggccaaat gccgaaaccg gcagaaccgc
      721 cgtaccggca agcaaggtga taaacgcgcg ccgcctcatc gtcgcctccg aggctcagaa
      781 aaatagatgc cgatcgctca aggtcgcccg tgttatactg agatattaaa tgatttcggc
      841 caaggaaaga ccacgcctcc aggccgaagt cacggcttcc agcgttcccg gagcctcaat
      901 gatggtcgaa aagaccggac gtaacggaat aactcggacg caccgacgtt tgtgctttgg
      961 gggacaaagg cgtcctccaa atgctatttt ggcaaaaaga cgagacttca agtggaggat
     1021 attcatgaag gcaatcagga cggtcgcaat tctttttggc ctggccgtca tggccggatc
     1081 ggccgctgcg caaaatgcgc cggcaacttc caccaagaag gtggatggca ccgataacgt
     1141 ctatattttc cgttacggca accatcaggc gatgttcgtc gtgacgccgc agggtgtcat
     1201 cgccaccgat ccaatctctt atcagcgtcc ggccaagcct tacatcgacg ccatcaaggc
     1261 cgtcaccgac aagcccatca aatacgtcat ctacagccat catcactacg accacatcgc
     1321 gggcgggcaa ccgttcaagg aacttggcgc gaccttcatc gcgcaccggc gcgcaaagga
     1381 gcgcctgctc gaattgaaaa agcagaatgc gctgctggct gacgtggtga tcccggatca
     1441 ggtcgtggac gacaaaaaga ccatcaagct cggcggcacc acgctcgaac tgatctatgt
     1501 cggccgcaat cattcggaca actcgctggt gatgcggttg ccaaaggaga agctcgtctt
     1561 cgtggtggat ttcgcgccga tcgagtcagt tcagttccgg aacattccgg acaatgcctc
     1621 gccgctggaa tacatcgatt cactcaagaa ccttgccgcg ctggattggg atcggctgat
     1681 cccgggtcac cccaacgccg gtgggcgcct tggcaccaag aaggatgtgc aagacgacat
     1741 tgcctacatg gaggacttgt cgtcggaagt gaagaaagca gcagacgcgg gcaagtgttt
     1801 cgacaccgcg atgaaagagg tcaagctgcc gaaatacgaa aaatgggcca attacgacgc
     1861 cagtttgccg gccaatgtgg agcgattctg ctattggtgg ggccgcggct attgatcagg
     1921 ccaaagacgc agcctcagca cgcttcactc tatcgcgccg cgcgaatgcg gacgcgcacc
     1981 ttgttgctct ttgacatgtt caatcggaaa ggcgcctccg tcgccttccc tctccggcac
     2041 gatcggaccg ccgcggaggc tcaaccctcc ggtgccgatc ggccggagac tgctgacttg
     2101 tcgcccggtc tcgtgatcgg cgggatgcaa ctaccaaagc gcgggtccag cggatcgaaa
     2161 cgtgaccttt gtctcgcgat aatgtagagc gtcgccagcg tcatcggacc gagcagcagc
     2221 atcaaccaaa cgggcggttg cagcgcgacc aggacggtcg gcgccgtaac cactgcggcc
     2281 acagcgatgc tgataatctg ccacgagcgc attgacctgc tccgctgcag acatcatttc
     2341 atgtgcgcgc gcggatcgtg tcaatcgctc ctggggcgga ccgggtgggc catattaccg
     2401 cagcttgttc agcagcgcga acagcgtctc gcgctccctc gcatcgagcg gcgccagcgt
     2461 ctccttcgag atcgcgactg cgttcggcgc ggccttctcc gcaagttgct ggccggcgcg
     2521 ggtgaggctc accagcagac ggcggccgtc ctcgggatcg gggctggtct cggtgagccc
     2581 ccgcgcggtg agccgatcga tcacgccctt gatcgtcgcc acatccatcg ccgtcagccg
     2641 gccgagctgg ttctgcgagc acggtcctgt ctcgttgagc ttggcgagtg ccgcccattg
     2701 cgtcggcgtc aggttgatgc cgatatcgcg cgcgaagatc gcggcatgac gctgccagac
     2761 ctggcgcagg atgaagccga tctgctcgtc gaggatgtac gacggcctcg acggcttgat
     2821 gctcttcttc agcgacgcaa tcctgcccat cggcaccccc tgctccctgg atcgtgcgca
     2881 aacataaact tacagcgaca gatgcgcatc gcggatatcc ggacgcgcat cgagctcgtt
     2941 catcgtgccg ccaaagcaga tccggccgcg ctcgatgata taggcgcgat cggagatcag
     3001 ccgcgcaaaa tgcaggttct gctcggagac gacaatgctg acgccttcct tcttcatggt
     3061 gaggatggcg tcgaccatct gttcgacgat ctttggcgaa agcccttcgg atggttcgtc
     3121 cagaagcacg agcgacgggt tgcccatcag cgttctggca atcgtcagca tctgctgctc
     3181 gccgccgctc atgcgtccac cgggacggtt gcgcatctcg ccgagattgg gaaacagcgc
     3241 gaacagtttt tcgcgcgtcc agtgcggcgc gttcggacgc tttgcctgac gaccgacttc
     3301 gagattttct tccacggtca gctcggtgaa gatgcgccgc tcctccggca catagccaag
     3361 tccctcgcgc acgatctcat gggtcggctt ccgcgagaca tcgttaccgt cgaacatgat
     3421 tcggcccgag cgctgctcga ccagaccgac gatggagcgg aaggtggtcg acttgccggc
     3481 gccgttgcgc ccgagcagcg cgaccacctc gccgttgccg acctcgagcg cgatgtcgaa
     3541 caggatgtgg gcggggccat aaaagctgtt gaggtcgcag accgagagct tcatgccgat
     3601 gctccctcgc ggtggcgcgc gtcatagacc agcccctcgc cgagatagat tgcgcggacc
     3661 tcctcgttgg cgcgcacctg ctccggcgtt ccttcggcaa tcaggctgcc gcggttgaga
     3721 accaggatgc ggtcggcatg ctcgaatacg acatccatgt cgtgctcggt gaagaggatg
     3781 ccgatggtct gctcgcgcgc gatgcgtgcg gtgagccgca tcaggtcgat gcgctcgcgc
     3841 ggtgccatgc cggcagtcgg ctcgtccatc agcagcagct tcgggttgtt ggcgagcgcg
     3901 atcgcaagct cgagccgctt gaggtcgccg taagcgagct cgccgcaggg gcgatctgca
     3961 tagccgctca tgccgaccag ctccagcagg cggccggcct gctcgcgcat caatttcggt
     4021 gtggaggcga cgaggttgaa cagacgtcgt tcatgcgaca ccagggcgac ctgaacgttc
     4081 tcgcgcacgg tcatggtcgg aaatgtcgcg gtgatctgaa aggtgcgccc cacgccgagc
     4141 cgccagatgt cgcgtggctt ttggcccgtg gtgtcctcgc cgagcaaggc gatgcggccc
     4201 tgatccgggg cgatctggcc gttgagcatg tcgaagcagg tgctctttcc tgcgccgttc
     4261 ggaccgatca gggccaggat ctcaccggcc ttcagttcga acgacacgcc gcgcacggca
     4321 tgcacgccgc cataggattt ggtgaggttc ttgaccgaca gcaatgtgga tgtgctgttc
     4381 attcggcggt ctcgacgcgg gaggagaaca gcgcggattt cagcgaggcg ccgcggcggc
     4441 ggccgatgga ctcgaccatg ccgacaatgc ctttggggaa ggcgacgacg atcagcacga
     4501 tgaaggcgcc gagcacgagt ttcgaccagt cggtctggct caccagccag atgctcagcg
     4561 ccttgtaaac gatcgcgccg atgacggcgc cggagacggt ttcgacgccg ccgagcagga
     4621 ccatgaccag cgcatcgacc gacagcgaga tgccaaggct gtcgggaaag acgctgccct
     4681 tcaaatacgc gaacagcgcg cctgcaacgc ccgcgacggt ccccgcgatc acgaaggcgg
     4741 tccactggat gcgcttgccg tcgatgccga tggcttcgcc gcgcaacggc gagtcacgcg
     4801 tcgcgcgcaa cgcaaagccg aacggcgaga acacgacgat gcggagcgca acgacgacga
     4861 gtgctgcgat cccgagcgag agccagtaga aatgcgacgg gcttgcggcc catttttccg
     4921 gccacacgcc cagaatgccg ttgtcgccgc cggtgacctc gacccactgg aacgcgatcg
     4981 accagacgat ctgggcaaac gcaagcgtca gcatggcaaa gtagacgccc gacaattgca
     5041 cggcaaagaa gccgaacacc gcagcgccca ggcagccgag caacggaccg agcaagaggc
     5101 acaggatcat cggcagacct gcgagctttg caagaaacgc aacgccgtat gcgccgaggc
     5161 cgaaataggc cgcatgaccg aaggatgcga gcccgccgac cgacatcagg aaatgcaggc
     5221 tcgcggcaaa gatcacgaag atcgcgatct cggagccgac ggtgagcgcg
//
LOCUS       DATLJT010000520         3123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_260685,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000520 DATLJT010000000
VERSION     DATLJT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260685"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM910_12050"
     CDS             <1..274
                     /locus_tag="VM910_12050"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI63299.1"
                     /translation="AIQHNPTYHYNPDYHFKGVRSKRAFTINPGPNNPVGTVWISLSA
                     EGYGLHGTPHPGKISTAESHGCVRLTNWDAERLAKQVKKGTPVVFG"
     gene            complement(343..1143)
                     /gene="thiD"
                     /locus_tag="VM910_12055"
     CDS             complement(343..1143)
                     /gene="thiD"
                     /locus_tag="VM910_12055"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HVI63300.1"
                     /translation="MATPAALTIAGSDSSGGAGIQADLKTFAALGVYGASAITALTAQ
                     NTRGVAGVHAVPADFVTAQIDAVFSDLDIGAVKIGMLAERAIIDAVADGLKRWSPKHV
                     VLDPVMVATSGDRLLAADAVEALRTQLIPLASILTPNLPEAAALLDARVASQEDAIVE
                     QGRRLLAYGCTAVLIKGGHGEGTESIDYLIDRQGTIALSAPRVATRNTHGTGCSLSSA
                     IAAGLARGEDLESAVRNAKAFISAAIAAADRFSVGHGHGPVHHFYRFY"
     gene            1245..1490
                     /locus_tag="VM910_12060"
     CDS             1245..1490
                     /locus_tag="VM910_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI63301.1"
                     /translation="MVPFFVQIKCKLGQSYVVANALAEAEIASEIYSTAGNYDLLVKF
                     YVDKDTDIGHFVNEKVQVIPGIQDTHTIITFKAFGTS"
     gene            complement(1570..2502)
                     /locus_tag="VM910_12065"
     CDS             complement(1570..2502)
                     /locus_tag="VM910_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI63302.1"
                     /translation="MLRRIFLSLLLAAAAAFAVYWWLTAPPPRLPVTTAAYSPKVGNG
                     EIIFNAGGCSSCHAVPGQPDRFRLGGGLAIPSPFGTFYAPNISPDPTDGIGRWSETDF
                     VEAVMKGVSPGGTHYFPAFPYPSYAHAKIDDVRDLFAYLKTLPPVAGKVRDHDVPFPF
                     NIRRNVGIWKLLFMDDRPLKPEAAHSASWNRGDYLVNSFGHCAECHSPRNFLGGIIAS
                     QRFAGGPNPEGEGWVSNITQKGLSEWSEKDIAYFLETGQTPDNDSAGGSMARVIKNTS
                     QLPPQDRNAIAEYLKSLPPVEGPPRPPKKPKSEG"
     gene            2691..3110
                     /locus_tag="VM910_12070"
     CDS             2691..3110
                     /locus_tag="VM910_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HVI63303.1"
                     /translation="MTKIMFATLALGAAVALAGPAFAEKFKVTLDGKSEVPPTTSTAT
                     GTADVDYDAASKKLTWKVTYSGLSGPATAAHFHGPAEAGKNAGVSVAIPNAGSSPVEG
                     SATLTDAQAADLQAGKLYINVHTAANPGGEIRGQVTK"
BASE COUNT          608 a          989 c          979 g          547 t
ORIGIN      
        1 cgccatccag cacaacccga cctaccatta taatcccgac taccatttca agggcgttcg
       61 atcgaaacgg gcctttacga tcaatccggg tccgaacaat cccgtcggca cggtgtggat
      121 cagcctgtca gccgagggtt atgggctcca cggcaccccg catcccggca aaatcagcac
      181 ggcggagtca catggctgcg tacgcctcac caactgggac gccgagcggc ttgcgaaaca
      241 agtgaagaag ggcacgccag tcgtttttgg ctagctgaag acacgtgtcg ctgatctctc
      301 gcacacgcgc gggagcaaac ttttagcggt cgcttcgccg catcaataaa agcggtaaaa
      361 atgatgcacc ggcccgtgtc cgtggccgac gctgaagcga tctgcggctg cgatcgccgc
      421 gctgatgaaa gccttcgcgt tacgcaccgc gctctccaga tcctcgcccc tggcaaggcc
      481 cgcggcaatc gccgacgaca gtgagcatcc ggtgccatgg gtgttgcggg ttgcaacgcg
      541 cggcgcggac agtgcaattg tgccctgcct gtcgatgaga tagtcgatac tctcggtccc
      601 ctcgccatgg ccgcccttga tcagcacggc ggtgcagcca tacgcgagca gtcgccggcc
      661 ctgttcaacg atcgcatcct cctggctcgc cacccgcgca tccaggagcg ccgccgcctc
      721 cggcaggttc ggcgtgagga tcgacgccag cggaatgagt tgtgtccgaa gcgcctcgac
      781 cgcgtcggca gcgagcagcc gatcgcccga ggtcgcaacc atcacgggat caagcacgac
      841 atgtttcggc gaccaccgct tcaggccgtc tgcgactgca tcgatgattg cgcgctccgc
      901 cagcattccg atcttgacgg cgcctatgtc gagatcgctg aacacggcat cgatttgtgc
      961 ggtgacgaag tcggcgggca cggcatgcac accggcaacg cccctcgtgt tctgcgcggt
     1021 caatgcggtg atggcggacg cgccgtagac accgagcgcg gcaaacgtct tcagatcggc
     1081 ctggataccg gcgccgccgg acgaatccga gccggcgatg gtgagggctg cgggcgtggc
     1141 catgccctgt cctaccccgg ccgcccggtg ccagcaagtt ggcttgctaa cgcccccaaa
     1201 tctggcctat ctggggtcgt tatatgcccc cggagacgac cgcgatggtg cctttcttcg
     1261 tccagatcaa gtgcaagctc ggccaatcct atgtggtcgc caacgcgctt gcggaggccg
     1321 agatcgcctc cgaaatctat tcgaccgcgg gcaattacga cctgctggtc aagttctacg
     1381 tcgacaagga cactgacatc ggccatttcg tcaacgagaa ggtgcaggtc attccgggca
     1441 ttcaggacac gcacaccatc atcaccttca aggccttcgg gaccagttag cgtttcggca
     1501 ggcgaattgt caggccgctg ctttcctgcc gcgaaagctg cgcggcgaac caaccaaagc
     1561 tccctgaact cacccttcgc tcttcggctt cttcggcggc cgcggcggtc cttcgaccgg
     1621 cggcagcgac ttcaaatatt ccgcgatcgc gttgcggtcc tgcggcggaa gctgcgaggt
     1681 gttcttgatc acgcgcgcca tcgagccgcc ggcgctgtca ttgtcgggcg tctggccggt
     1741 ttcgaggaaa taggcgatgt ccttctcgct ccactcggag aggcccttct gagtgatgtt
     1801 cgacacccag ccttcgcctt ccggattggg cccacccgcg aagcgctggc ttgcgatgat
     1861 gccgcccaaa aaattgcgcg ggctgtggca ttcggcgcaa tggccgaagc tgttgacgag
     1921 atagtcacca cgattccagg acgccgagtg cgcggcctcc ggcttgagcg gcctgtcgtc
     1981 catgaacaac aacttccaga tgccgacgtt gcggcggatg ttgaagggga acggcacatc
     2041 gtgatcgcgc accttgcctg cgacaggcgg cagcgtcttc agataggcga agagatcgcg
     2101 gacgtcgtcg attttggcgt gcgcgtagga cggataaggg aaggccggga aatagtgcgt
     2161 tccgccgggc gacacgcctt tcatgaccgc ctcgacaaaa tccgtctcgc tccatcgccc
     2221 gatgccgtcg gtcggatcgg gcgagatgtt gggcgcatag aacgtgccaa aaggcgacgg
     2281 gatcgcaagc ccgccgccca gccggaagcg atcgggctgg cccggtaccg catggcagga
     2341 cgagcacccg cccgcgttga agatgatttc gccatttccg actttcggcg aataggccgc
     2401 cgtggtgacc ggcaggcggg gcggcggcgc ggtcagccac caatagacgg cgaaggcggc
     2461 agcggcggcg aggagcagag acaggaaaat tcgtcgcagc attaccgatc cgtcgttgcg
     2521 ggaagagatc cgggctagta tggccagacc gacgtcttcg ctccagactt gtatttcaga
     2581 cggatcgggg gaaaattttg cggcggttga gcgggttctg ggaataaacg gagcttcgca
     2641 atgttggaaa agcggcgcca tcaattgacg tcaacaggga gagagagacc atgaccaaga
     2701 tcatgtttgc cacgcttgcg ctcggggcgg cggttgccct cgcaggccct gcgttcgccg
     2761 agaaattcaa ggtgacgctg gacggcaaat ccgaagtacc cccgacgacc agcacggcca
     2821 ccggcacggc ggacgtagac tatgatgccg cgagcaagaa gctgacctgg aaggtcacct
     2881 attccggtct ctccggtccc gccacggccg cgcatttcca tgggccggcc gaagccggca
     2941 agaacgcagg cgtgtcggtt gcgatcccca acgccggctc gagcccggtg gagggcagcg
     3001 cgacgctgac cgatgcccag gcggccgacc tacaggccgg caagctctat atcaacgtcc
     3061 acaccgcggc caatccgggc ggcgaaatcc gcggccaggt gaccaaatag cgggaaggtc
     3121 cga
//
LOCUS       DATLJT010000521         4444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3706614,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000521 DATLJT010000000
VERSION     DATLJT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706614"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VM910_12075"
     CDS             <1..856
                     /locus_tag="VM910_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVI63304.1"
                     /translation="GSLNRLSRLGVNAVGIPLDREGMRMDALASALADLKARGIRPKY
                     IYTIPTVQNPTGSILPEARRAEMLRLSQQYGVPIFEDDCYADLVWDGKRPPAIHAMAK
                     NGGVIHIGSFSKSIAPALRVGFIVAPWEMMSRMLALKTDAGSGALEQMVLAEYCTPHF
                     TDHVPKLTRGLHAKLDTLMEALNEQFGTSAEFDAPKGGIFLWVKLPDNVDTLKLYQSA
                     LAAGVAINPGPEWSTNRAHSSSRLRLCFASPSHEQIREGVAVLADVCRREFGVPVRIA
                     NVEKRARG"
     gene            complement(881..1735)
                     /gene="hpaD"
                     /locus_tag="VM910_12080"
     CDS             complement(881..1735)
                     /gene="hpaD"
                     /locus_tag="VM910_12080"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HVI63305.1"
                     /translation="MGQLVLAAKVTHVPSLMLSEAEGSPLQRARSGAVAALRELGRRA
                     RARGVSTFVVFDTHWLSNFGYHINANPQHRGAFTSHEAPQMIQNLRYDLPGNTALAEA
                     IAKEAACDGLNVMAHKVESLGLEYGTIVPMHYMNANGWAKVVSVASPLFTSLEESRVL
                     GEATARAIEKSGERVALLASGSLSHRLLPNKKLGPEAWISIASEFNRQVDLRVLELWE
                     QRRYREFLDMLPDYATKCNGEGGMADTIMLFAALGWYDYRGAAEQLCDYFPSSGSGQV
                     NVEFHLDM"
     gene            1933..2394
                     /locus_tag="VM910_12085"
     CDS             1933..2394
                     /locus_tag="VM910_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63306.1"
                     /translation="MQSAGTDFTRLSLALGLLVVLVSQATAESPADTATRWGLIGPWS
                     LDCALPADRGAGMVLSYEIAGGDRLIHRRYFGDGSDESEVVGATISADGVLNLRIHFP
                     ALKETRELGLMKLSDDGMRAVYNRSENGRYSIKGGKFTATGKPTPVQHKCR"
     gene            2491..2721
                     /locus_tag="VM910_12090"
     CDS             2491..2721
                     /locus_tag="VM910_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63307.1"
                     /translation="MRKFGYVIAALGAIAVAAPSIASADTVVIKRGHNHWEGARAEFR
                     DHRDYGWHRGWYRDHDRDRGGVVIREHRYDRY"
     gene            complement(2805..3623)
                     /locus_tag="VM910_12095"
     CDS             complement(2805..3623)
                     /locus_tag="VM910_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI63308.1"
                     /translation="MDYLQTQGLRIPRLGLGTFRMLGDACRAAVESALQLGYRHIDTA
                     EMYGNEDAIGVAIAASGIKRADLHVTTKVWNENLAPDAIRRAFDASLNKLRLDTVDLY
                     LVHWPSRRMNLPAIFETLMKLKEEGRTRAIGVANFNVALLKTVVEEIGAPIACDQIEY
                     HVMLDQSKVKAYLDAKSIPLIAYCPLAQGRVASNETLIAIGKKHDASAAQVALKWLLD
                     QHGVAAIPKASRRESQKANLDALGVRLDDEDRRKIAGMPKNLRCVSPAFAPAWD"
     gene            complement(3767..>4444)
                     /locus_tag="VM910_12100"
     CDS             complement(3767..>4444)
                     /locus_tag="VM910_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVI63309.1"
                     /translation="GRIYRRISYGPLLDIFLIDMRSYRDSTWNKGDDRDSFILGATQL
                     AWLKRELTSSNATWKVIAADLPIGIVSTDAIALGDGPPARREHEIADLLSFLKHAGVG
                     NVVWLTADMHYTSAHYYDPNHAVFRDFEPFWEFVSGPLHAGTWSPGQLDNTFGPRAMY
                     RKGCDCEQGDNLAPCFGLQFFGRVDIDGKTGVMTVTLKDVEDNDLWSVDIAPKPDARL
                     LQRVTSR"
BASE COUNT          831 a         1396 c         1387 g          830 t
ORIGIN      
        1 gggctcgctg aaccggctct cccggctcgg cgtgaatgcg gtcggcattc cgcttgaccg
       61 cgagggcatg cggatggacg cgctggcctc ggctcttgcc gatctcaagg cgcgcggcat
      121 ccggccaaaa tatatctaca ccattccgac cgtgcagaac ccgaccggca gcattttgcc
      181 ggaggcgcgc cgcgccgaga tgttgcggct ctcgcagcaa tacggcgtgc cgatcttcga
      241 ggacgattgc tatgccgacc tcgtctggga cggcaaacgc ccgcccgcga tccacgccat
      301 ggcgaaaaac ggcggcgtga tccatattgg ctcgttctcc aaatcgatcg cacccgcgct
      361 ccgcgtcggc ttcatcgtcg cgccctggga gatgatgtcg cggatgctgg cgctgaagac
      421 cgacgccggt tccggcgcgc tggagcagat ggtgctggcc gaatattgca cgccgcattt
      481 caccgaccat gtgccgaagc tgacacgcgg tctgcacgcc aagctcgaca cgctcatgga
      541 agcgctcaac gaacagttcg ggacctcggc cgagttcgat gcaccgaagg gcggcatttt
      601 cctctgggtg aagctgcccg acaatgtcga tacgctgaaa ctctaccagt cggcgctcgc
      661 cgccggcgtc gcgatcaatc cggggcctga atggtcgacc aacagggccc attccagcag
      721 ccggctgcgg ctttgctttg cgagcccgtc gcacgagcag atccgcgagg gtgtcgccgt
      781 gctcgccgac gtttgccggc gcgagttcgg cgtgccggtc cgtatcgcca atgtggagaa
      841 gcgggcgcgc gggtgagcgt caatcgatca aattgaatac tcacatgtcg aggtgaaact
      901 cgacattgac ctggccgctg ccgctggagg gaaaatagtc gcagagctgc tcggctgcgc
      961 cgcgatagtc gtaccagccg agtgcggcga acagcatgat ggtgtcggcc atcccgccct
     1021 cgccgttgca cttggtggcg tagtccggca gcatgtcgag aaactcccga tagcggcgct
     1081 gctcccatag ttcgagcacg cgaagatcga cctgccggtt gaactcgctg gcgatcgaaa
     1141 tccaggcttc cgggccaagc ttcttgttcg gcaacagccg gtgcgacagc gaacccgagg
     1201 caagcagcgc gacacgctcg ccggattttt cgatcgcgcg cgccgtggct tctccgagca
     1261 cgcggctttc ctccagcgag gtgaacagcg gcgatgcgac cgagacaacc ttggcccagc
     1321 cgttcgcatt catatagtgc atcggcacga tcgtgccgta ttcgagcccg aggctttcga
     1381 ccttgtgcgc catgacgttg aggccatcgc aagccgcctc cttcgcgatc gcttcggcca
     1441 gtgccgtatt gccgggcaga tcgtagcgca ggttctggat catctgcggt gcttcgtggc
     1501 tggtgaaggc accgcgatgc tgcggattgg cgttgatatg gtagccgaaa ttggacagcc
     1561 agtgcgtatc gaacactaca aaggtcgaaa cgccgcgcgc cctggcacgg cggcccaact
     1621 cgcgcagcgc cgcaaccgca ccggaccgcg cacgttgaag cggactgccc tcggcttccg
     1681 acagcatcag cgacggcacg tgggtgacct tggcggcgag aacaagctgt cccaatcacg
     1741 acctcctttg agacgaaacc gacgacctcg caggaaaggg gaaggccttg tccaccgcat
     1801 tgatgcggat caaagcggcg ctggaacgtc ggtttgccag cgtgatcttc tgccgcgaat
     1861 ccgatttcgg cggcattcac cgccgtcgcg ccggcatgct aaattgtgca atgaagcgga
     1921 ggatttttcg ctttgcaaag tgccgggaca gatttcacga ggctctcgct cgcactcggc
     1981 ttgcttgtcg tgctcgtttc gcaggcaacc gcggagagcc cggcggacac cgccacgcga
     2041 tggggattga tcggcccgtg gtcgctcgat tgcgccctgc cggccgaccg cggcgccggc
     2101 atggtgctgt cctacgagat cgcaggcggc gatcgcctga ttcaccgccg ctatttcggc
     2161 gacggctccg acgaaagcga ggtcgtcggc gcaacgatct cggctgacgg cgtgctcaac
     2221 ttgcgcatcc acttcccggc gctcaaggaa acgcgcgagc tcggactgat gaagttgtcc
     2281 gacgacggca tgcgtgcggt atataaccgc agcgagaacg gccgttattc catcaaaggg
     2341 ggcaaattca ccgccactgg caagccgacg ccggtgcaac acaagtgcag atgaacgaag
     2401 ctgaatagct gttcagaatt ctcttcaggt tccatcggaa cgttgcttct cactcgaagt
     2461 tctaatctga ctgagagtga ggaggacatg atgaggaaat ttggttatgt catcgcggcg
     2521 cttggtgcga tcgcggtcgc ggcgccgtca attgcgagcg ccgacacggt cgtgatcaag
     2581 cgcggccata atcactggga aggcgcgcgc gccgagttcc gcgatcatcg tgactacggc
     2641 tggcacagag gctggtaccg tgatcacgat cgcgaccgcg gcggagtcgt gatcagggag
     2701 caccgctacg atcgctacta aagcgaacga gcgaggccga cacaggaatg gccccgaaag
     2761 gggccattct ttttctttgg cttgagcgat gctctcgcca caggctaatc ccacgccggc
     2821 gcgaaagcgg ggctgacgca gcggagattt ttcggcatgc ctgcgatctt gcgccgatcc
     2881 tcgtcgtcga gccggacgcc gagcgcgtcg agattggcct tttggctttc gcggcgcgac
     2941 gccttcggga tcgccgcgac gccgtgctgg tcgagcagcc atttcagtgc aacctgcgca
     3001 gcactcgcgt catgcttttt gccgatcgcg atcagcgtct cgttcgaggc gacgcggccc
     3061 tgcgccagcg gacaataggc gatcagcggt atcgacttcg cgtcgagata ggccttcact
     3121 ttgctctgat cgagcatcac gtgatattcg atctggtcgc aggcgatcgg cgccccgatc
     3181 tcctcgacca cggtcttgag cagcgccacg ttgaaattgg cgaccccgat ggcgcgggta
     3241 cgcccctcct ccttcagctt catcagcgtc tcgaagatcg ccggcaggtt catccgccgc
     3301 gacggccaat gcacgagata aagatcgacg gtgtccaacc gcagtttgtt caggctggca
     3361 tcgaacgcgc ggcggatcgc atccggcgca agattctcgt tccagacttt cgtcgtcacg
     3421 tgcagatcgg cccgcttgat cccggacgcc gcgatcgcaa cgccaatcgc gtcctcgttg
     3481 ccgtacatct cggcggtatc gatgtggcga tagccgagtt gaagcgcgct ttccaccgcc
     3541 gcgcggcagg catcgcccag catgcggaat gtgccgagcc cgagccgcgg gatgcgcagc
     3601 ccctgggttt gcagataatc catgatcgca cctcaaaaac ctgatcgcct cgcggcaaag
     3661 ctcaaaacgc gctttctgtc ggagggttgc aaacggcgta aggcgcggag tttgcccgca
     3721 atcttcacgt cagatcaaga tcgcgtctcg ccgcgcatac cgatcatcaa cgactcgtga
     3781 cgcgctgcag cagccgcgcg tcgggcttgg gcgcgatatc gaccgaccag aggtcgttgt
     3841 cttcgacgtc cttcaacgtc accgtcatca cgccggtctt gccatcgatg tcgacgcggc
     3901 cgaagaattg caggccgaaa cagggagcga ggttgtcgcc ctgctcgcag tcacacccct
     3961 tccggtacat cgcccggggg ccaaacgtgt tgtcgagctg ccccggcgac caggtgccgg
     4021 catgcagtgg tcccgaaacg aactcccaga acggctcgaa atcccggaag accgcatggt
     4081 tcggatcgta ataatgcgcc gatgtgtagt gcatgtcggc cgtcagccac acgacgttgc
     4141 cgacgcccgc gtgcttgagg aaagacagga gatccgcgat ttcgtgctcg cgccgcgcag
     4201 gcggtccgtc gccaagtgcg atcgcatccg tcgagacgat gccgatcggc aggtcggctg
     4261 cgatcacctt ccaggtcgcg ttcgaagacg tcaactcgcg cttcagccag gcaagctgcg
     4321 tcgcgccgag aatgaagctg tcgcggtcgt cgcccttgtt ccaggtcgaa tcgcgatagc
     4381 tgcgcatgtc gatcaggaag atgtcgagca gcgggccgta ggaaatccgg cggtagatgc
     4441 ggcc
//
LOCUS       DATLJT010000522         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3477094,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000522 DATLJT010000000
VERSION     DATLJT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477094"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..742
                     /locus_tag="VM910_12105"
     CDS             41..742
                     /locus_tag="VM910_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HVI63310.1"
                     /translation="MNFACFRCLALAALIPAAAMLLSGGAVVAQQPPASPSPGVSTSP
                     SPAPPASPAPPANPSPAPAAPPPAAQTPAPSATVQTADPFGEAITLEAKKVVIAKGNA
                     NWDSAFDTLIDSFKGLSALLDKQGIKRSGNLMIVYTSTDDTGFTFLAEIPVEQDPKNL
                     GKGMSIGQSPDGKALKFVHRGSYDNMDNTYEAITNHLDDKKLEAKDTFIEEYVTDPLK
                     TAEDKLVINVYVPLK"
     gene            739..1452
                     /locus_tag="VM910_12110"
     CDS             739..1452
                     /locus_tag="VM910_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HVI63311.1"
                     /translation="MNQRLRFCLGAAVAAAALLATPALAQSDFPPAISVTGEATISVP
                     PDLAHIDAGVASDGKTAREASEANNAAMAKVLAALKGAGMDEKDFQTSRLSLQPQYAP
                     NRTGASPIVGYRASNRVAIRLRDVTKVAGVIDTLVGAGANDIGGVSFSVSQTSRLLDD
                     IREQAIADARRKAEIYAKAAGVTLGGPLSISEAGAAIPLFRAKVAGAPMAAAPTPIAQ
                     GEETLSLSVNVTWAIKQAQ"
     gene            complement(1520..>2284)
                     /locus_tag="VM910_12115"
     CDS             complement(1520..>2284)
                     /locus_tag="VM910_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63312.1"
                     /translation="HASWLIQASGLNILVDPVWSMRASPVSFAGPKRHNDPGIAFEAL
                     PRIDIVLVSHGHYDHLDVATLSKLAKTFAPRVITPLGNDLIMREADGAIKAEAFDWHD
                     RIELGNAIAVTLVPTRHWSARGLFDRNKSLWASFVLETPAGKLYIVCDSGYGEGKHFR
                     RVADAHGPLRLAILPIGAYEPRWFMRDQHMNPSDAVKALADCGAEQALAHHHGTFQLT
                     DEAIEAPAFALAQALDEARLPREKFAVLKPGQVYEI"
BASE COUNT          438 a          779 c          686 g          381 t
ORIGIN      
        1 ggaagcctga ctgcctgaca cgccccatgg agaatactgg atgaatttcg cctgctttcg
       61 ctgccttgcc ctggccgcac tgatcccggc agcggcgatg ctgctctccg gcggcgcggt
      121 ggtggcgcaa cagcccccgg cttccccctc gcccggggtc tcgaccagcc cctcgcctgc
      181 acccccggca agccccgcac cgcccgcgaa cccctcgcct gctcccgccg ccccgccgcc
      241 ggccgcgcag acgcctgcgc cttccgcaac ggtccagacc gccgatccct ttggcgaggc
      301 gatcacgctg gaagcgaaga aggtggtgat cgccaaaggc aacgccaact gggattcggc
      361 gttcgacacc ttgatcgatt ccttcaaggg gctgagcgcg ctgctcgaca agcagggcat
      421 caagcggtcc ggcaatctga tgatcgtcta tacgtcgacc gacgataccg gcttcacctt
      481 ccttgcggaa attccggtcg agcaggaccc gaaaaatctc ggcaagggta tgagcatcgg
      541 tcaatcgcct gacggcaagg ccctcaaatt cgttcaccgc gggtcctacg acaacatgga
      601 caatacctat gaggcgatca ccaatcacct cgacgacaag aagctcgagg cgaaagacac
      661 gttcatcgag gaatacgtca ccgatcccct gaagaccgcg gaggacaagc tcgtgatcaa
      721 cgtctatgtg ccgctgaaat gaatcagaga cttcgttttt gcctcggcgc ggccgttgcg
      781 gccgccgcgc tgcttgcgac acccgcgctg gcccagtcgg attttccgcc ggcgatttcc
      841 gtcaccggcg aagcgaccat ttccgtgccg ccggacctcg cccatatcga tgcgggcgtc
      901 gcctcggatg gcaagaccgc gcgtgaggca tccgaggcca acaacgcggc gatggccaag
      961 gtgctggccg cattgaaggg cgccggcatg gacgaaaagg attttcagac ctcgcgtctg
     1021 tcgctgcagc cgcaatatgc gccgaaccgg acaggcgcct cgccaatcgt cggctatcgc
     1081 gcctccaatc gcgtggccat ccggctccgc gacgtgacca aggtcgcggg cgtgatcgac
     1141 acgctggtcg gcgccggagc caatgatatc ggcggcgttt ccttctcggt ctcgcagacc
     1201 tccaggctgc tcgacgacat cagggaacag gccatcgccg atgcacgccg caaggccgaa
     1261 atctatgcca aggctgccgg cgtcactctc ggggggcctt taagcatttc ggaagccggt
     1321 gcggcgatcc cgctgtttcg cgccaaagtg gcgggcgccc cgatggccgc tgcaccgacg
     1381 ccgatcgcgc agggcgagga gacgctgtca ctgagtgtca acgtgacctg ggcgatcaag
     1441 caggcacaat agacattcga ccagcgaaga tcgtcatgcc cggccttgtg ccggccatga
     1501 cgacccaaca gagacggcgt caaatctcgt acacctgccc cggcttcaac accgcaaatt
     1561 tctcgcgtgg cagcctcgct tcgtccagcg cctgcgccag cgcgaatgcc ggagcctcga
     1621 tcgcctcatc ggtgagctgg aacgtgccgt gatgatgcgc gagcgcctgc tcggcaccgc
     1681 aatcagccag cgccttgacc gcgtccgacg gattcatgtg ctgatcgcgc atgaaccagc
     1741 gcggctcata agcgccgatc ggcaggattg cgagcctgag cggaccatgc gcgtccgcca
     1801 cgcggcggaa atgcttgccc tcgccataac cggaatcgca gacgatgtag agcttgcccg
     1861 cgggcgtctc cagcacgaag ctcgcccaca acgatttgtt gcgatcgaac aggccgcgcg
     1921 ccgaccagtg tcgcgtcggc accagcgtca ccgcgatcgc attgccaagc tcgatccgat
     1981 cgtgccagtc gaatgcctcg gctttgatcg ccccatccgc ctcgcgcatt atcagatcat
     2041 tgccgagcgg cgtgatcacg cgcggcgcga acgtcttggc gagtttcgac agagttgcca
     2101 cgtcgagatg gtcatagtgg ccgtgcgaga ccagcacgat atcgatccgg ggcagcgcct
     2161 cgaaagcaat accgggatcg ttatgccgct tcggcccggc aaaggaaacc ggcgaagccc
     2221 gcatcgacca cacgggatcg accaggatgt tcagcccgga ggcctggatc agccagctcg
     2281 cgtg
//
LOCUS       DATLJT010000523         3798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1639029,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000523 DATLJT010000000
VERSION     DATLJT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639029"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VM910_12120"
     CDS             complement(<1..820)
                     /locus_tag="VM910_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HVI63313.1"
                     /translation="MAELAVDRRPPDLTIVAPPAPASQRYINRELSWLAFNERVLDEA
                     SNTGHPLLERVRFLSISDINLDEFYMVRVAGLQELAESGITVKSDDGLTPEQQLAAIA
                     EVAGGLVHWQHQCWVDLQKRLRAEGISIVDHDALTVDDRSWLRQLFIDRLLPVMTPIA
                     IDPAHPFPFIPNRTLAMVVEFHPDNGSRVNALVLVPERVERFIRLPGKEIRFISLEEV
                     LLAVGLLELFPGMTPLGHGMFRALRNSDIEIEEKAQDLVAMFESALKRRRRGEIT"
     gene            complement(861..1535)
                     /locus_tag="VM910_12125"
     CDS             complement(861..1535)
                     /locus_tag="VM910_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI63314.1"
                     /translation="MHAVDVMVRDVVTVRSDTDVADAVELLSQHDVSALPVVDEAGNL
                     VGILSEADLIHRVEIGTEKHRPWWLEAVTGASTLAEEFAKSHGKKVREIMTSGAISVS
                     EETPLAEIAALFERKRIKRVPVVKDGKLVGIVSRSNLIQALASVVGRMDQHDETDRQI
                     RLELLSRLKDQPWTGFGDRNITVGNRVVHLWGLVGSEAERKALLALAERVPGVSKVSD
                     EMIPAY"
     gene            complement(1555..3174)
                     /locus_tag="VM910_12130"
                     /pseudo
     CDS             complement(1555..3174)
                     /locus_tag="VM910_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            3313..>3798
                     /locus_tag="VM910_12135"
     CDS             3313..>3798
                     /locus_tag="VM910_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004116285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter permease"
                     /protein_id="HVI63315.1"
                     /translation="MQGSSTSYASLTARGLVPNRWDIIAFPLVFGLLLLAAKGARETL
                     APLSALKEPVISLDPINLPEYALRTTLRMLAAMVASLVFTVTYGTLAAKSRRAELVLV
                     PLLDILQSVPVLGYISFTVVFFMSLFPGRVAGAEAAAIFAIFTSQAWNMTFSFYQSLR
                     TV"
BASE COUNT          755 a         1185 c         1116 g          742 t
ORIGIN      
        1 gagtgatctc accgcgccgc cgccttttga gcgcagactc gaacatcgcc accaggtctt
       61 gcgctttttc ttcgatttcg atgtcgctgt tgcgcagtgc tcggaacatg ccatggccga
      121 gcggcgtcat cccgggaaac agctctaaca gccctaccgc gagcagcact tcctcaagcg
      181 agatgaagcg aatttccttg ccgggtagcc ggataaaccg ctcgacgcgt tccggaacta
      241 gtaccagcgc attgacccgg gacccattgt ccggatgaaa ttccacgacc atcgccaagg
      301 tacgattggg gatgaaaggg aacggatgcg ccgggtcgat cgcgatcggc gtcatgaccg
      361 gaagcaagcg atcgataaag agttgccgca gccaagagcg atcgtcgacc gtcaacgcgt
      421 cgtgatcgac aatggagata ccctcggcac gcaggcgctt ctgcagatcc acccagcatt
      481 ggtgctgcca atgaactagg cctcccgcga cctcggcgat tgcggccagt tgctgctccg
      541 gggtcaagcc gtcatcgctc ttcacggtga tcccgctctc tgccaactcc tggagcccgg
      601 cgacgcgcac catgtagaat tcgtcgaggt tgatgtcgga gatcgacaaa aaccgcactc
      661 gctcgaggag cggatgaccg gtattgcttg cttcatcgag aacgcgctcg ttgaaggcaa
      721 gccacgacaa ctcgcgattg atgtatcgct gcgacgcagg cgccggcggc gcgacgatcg
      781 tgaggtcggg agggcgacgg tcgaccgcca attcagccat ttgcaccctc caatttaacc
      841 gtgggcccta tgcgctcctg tcaataggcc ggtatcattt cgtcggaaac cttcgacacc
      901 ccgggcacgc gttccgcaag cgctagcagc gccttgcgtt cggcctccga gccgacgagc
      961 ccccagagat gcacgacacg gttgccaaca gtaatgtttc gatcgccaaa accggtccac
     1021 ggttggtctt tgagtcgaga cagcagctcg agccggattt ggcggtccgt ctcgtcgtgc
     1081 tggtccatac ggccgaccac cgatgcgagc gcttggatca gattcgaccg gctgactatg
     1141 ccgacgagtt tgccgtcttt gacgaccggc acgcgtttga tccgctttcg ctcgaacagc
     1201 gcggcgatct ccgcgagtgg cgtttcctcg gacaccgaaa tggcgcccga ggtcatgatt
     1261 tcgcggacct tcttgccatg cgacttagcg aactcttcgg cgagcgtgct tgcgccggtc
     1321 accgcctcga gccaccacgg tcgatgcttt tcggtgccga tttcgacgcg atggatcagg
     1381 tccgcctcgc tgaggatacc aaccaggttg cccgcttcgt cgacaactgg aagagcgctg
     1441 acgtcgtgct gcgacaagag ctcgaccgcg tcggccacgt cggtatcgga acgaacggtc
     1501 acgacatcgc gcaccatgac atcgacagcg tgcatgcccc cctcctgggt ccgctcagaa
     1561 caggttaagg aacaggatgt agaggcccgc cgacagcatc atcgccaccg gcaaggttaa
     1621 tatccacgcc attacgatgt tgcggagtgt cgacaattgc aggcccgacc cattggctgc
     1681 catggttccc gctacacccg atgacagaat atgggtggtc gacaccggca gaccgtacat
     1741 gtcggccccg gcgatggtca ccgccgcgac aagctccgca gcggcacctt gaccataggt
     1801 caaatgtgtc ttgccgatct tctccccgac agtgatgacg atccgcttcc atccgaccat
     1861 cgtgccgagg ccgagcgcaa tcgcgaccgc gatcttgacc cagatcggga tgaacttcgt
     1921 ggaggcgtcg agcgactgct tttaggcatt cagactcgcc acttcgtctt tcgacagatc
     1981 gctatccttg tccttcatca gattgcgcag agcctcatcg gcgagataca tatcgttgcg
     2041 ggtgttgccg actgcagcag cggggatttt tgcaatcgac ccgtactgct ggacctcttt
     2101 gtcgatgtcc tggatcagca cagcgagcga cggatacgtt ccctcgttga tcttgtggtg
     2161 cgcaacatac tccatcaccg cagggcgcgg gtcgccgagg atgttatacc cggcagcctt
     2221 ttcttcgacg atcttcgcgg cagccgccga gttggcctcg aactgcgcga tttggctgtc
     2281 gggcagggcg cggttgaggg cgaaggcggt ggggaccgtg ccgatcagga tcagcatgat
     2341 cagacccatg cccttctgcc cgtcgttgga gccgtgggcg aagctgacgg ctgtgcaggt
     2401 gaagaccagc agtccccgga tccaccatgc cggaggcttg tcggtttcgg gcgagcggta
     2461 caactcgggt tgtcgtatga gaagcttcat gacgagcagc aaaaccgccg agacaacgaa
     2521 gccgacgaca ggtgagaaca gcagcgcctt cccaacattg gtggcctgtc cccagtcgac
     2581 accggaggtt ccatcctggc cgtgtatcaa ggcgttggcg acgccgaccc cgatgatcga
     2641 gccgatcaag gtgtgcgagc tcgaagccgg caggccgaga taccaggtac cgaggttcca
     2701 gatgatcgcg gcgatcagca gcgcgaacac catcgcaaac cctgccgaac tcccgacctg
     2761 aaggatcagc tctaccggca ggagcgagat tatgccgaat gcgaccgcgc cgctcgacag
     2821 caggaccccg agaaaattga aactccccga ccatatgacc gctatctcgg ccggcagcga
     2881 gtgtgtgtag atcaccgtgg ccacggcatt cgcggtgtca tggaaaccat taacgaactc
     2941 gaagccgagg gcaatcagca gcgcgacgaa aagcagcagg tagggtaaaa acgtcgtggt
     3001 attggtgccg ctttcggcga tgtcggaata gagactgtac gccacaaaca gcagagcacc
     3061 tgcgagcagc agaaggaaga tgatagcggt gccaggattg agcccgccgt cgagcctcgg
     3121 gcgaacggcg atggcgccag tcccggcttc tgcgtgcagc gcaacgtcag ccatgttttc
     3181 tgatcccctg gttccggcaa gcaccggatt atggctagtt aatatttcag ttccgtgaca
     3241 gcgaccgctc agatctcgac ttgactatcg cgctgccccg tgctgcgagt ccgccgctca
     3301 aaggatacca gggtgcaagg aagctcaacc agctatgcaa gcctgacggc gcggggtctg
     3361 gtcccgaacc gctgggacat cattgccttc ccgttggtct ttggcctgct gctgcttgcc
     3421 gccaaaggag ctcgcgaaac cttggcgcca ctgtcggcgc tcaaagagcc cgtcatctcg
     3481 ctcgatccga tcaatctgcc ggaatacgcg ctgcgcacga ccttgcgcat gttggcggcg
     3541 atggtcgcct cgctggtctt caccgtcacc tacgggacac tcgccgccaa gagtcggcga
     3601 gccgaactcg tcctcgtccc actgctcgac atcctccaat cggtgccggt gctgggttac
     3661 atttccttca ccgtcgtctt cttcatgtct ctgtttcccg gccgggtcgc gggtgccgaa
     3721 gccgcggcga tcttcgcgat cttcacgagc caggcctgga acatgacctt cagcttctac
     3781 cagtcgctgc gcaccgtg
//
LOCUS       DATLJT010000524         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3247698,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000524 DATLJT010000000
VERSION     DATLJT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3247698"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2025
                     /locus_tag="VM910_12140"
     CDS             <1..>2025
                     /locus_tag="VM910_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVI63316.1"
                     /translation="DELCAMLDEWKIFWEWRDLPHEVWDFIKRKKFFGMIIPKEFGGL
                     GFSPYAHSEVVRKLSSRSLTAAVTVMVPNSLGPGELLMRFGTKEQQERWLPRLADGRD
                     IPCFGLTSPEAGSDAASMIDSGIICKGTFEGREMLGLKLNWHKRYITLGPVATLLGLA
                     FKAYDPDHLIGEVDELGITVALIPTHLPGVSIGRRHLPAMQVFQNGPNWGRDVFIPLD
                     YIIGGRERLGQGWKMLMTALAAGRGISLPSLSAAGAAYAARTTGAYARIREQFGISIS
                     KFEGIEEPLARIAGTAYLLDGARRLTCAALNEGHHPAVISGIMKLQATDRMRIVIDDA
                     MDIHGGKAIIDGPQNYLGNLYRSVPVGITVEGANILTRNLIVFGQGAIRAHPYLLDEM
                     NALRDDDRERGLDAFDRAFWKHVGHTFTNLFRAWGRSWSFGLFAPAPDASDATRFYRQ
                     LSRYAAGFALAADLALLTLGGALKRKEMLSARFGDILSELYLLSGALKRWQDEGRQRD
                     DFAALEWCMANGFRTIESRLAEICANLPNRLVGALLKFFLQPFGARVLGPSDRVVHQC
                     AQLVLEPSAARDRLTSDLSHVADDGGQARLEKAFVLVASTEEISKRLHAARLRDVNEA
                     VKKGVITQSEAERLAAAHEAVAKVIDVDDFAPEALSPIYRKPADVHQFFQ"
BASE COUNT          342 a          667 c          636 g          380 t
ORIGIN      
        1 cgacgagctc tgcgccatgc tcgacgaatg gaagatcttc tgggaatggc gcgacctgcc
       61 gcatgaggtc tgggacttca tcaagcgcaa gaagttcttc ggcatgatca tcccgaagga
      121 gttcggcggc ctcggcttct caccctatgc gcattcggaa gtggtgcgca agctgtcgtc
      181 gcgttcgctc accgcagccg tcaccgtgat ggtccccaat tcgctcgggc ccggcgaact
      241 cctgatgcgc ttcggcacca aagagcagca ggagcgctgg ctgccccggc ttgccgacgg
      301 gcgcgacatt ccctgcttcg ggctgaccag cccggaagcg ggctccgacg ccgcctcgat
      361 gatcgatagc ggcatcatct gcaaaggcac tttcgaaggc cgcgagatgc tggggctcaa
      421 gctcaactgg cacaagcgct acatcacgct cggccccgtt gcgacgctgc tcggcctcgc
      481 cttcaaggcc tacgatcccg atcacctcat cggcgaggtg gacgagctcg gcatcactgt
      541 ggcgctgatt ccgacgcatc tgcccggcgt ctcgatcggc cgccggcatc tgccggcgat
      601 gcaggtgttc cagaacggcc cgaactgggg ccgcgacgtc ttcatcccgc ttgattacat
      661 catcggcggc cgcgagcggc tggggcaggg ctggaagatg ctgatgacgg cgctcgccgc
      721 cggccgcggc atttcgctgc catcactgtc ggccgcgggc gcagcctatg ccgcgcggac
      781 caccggcgcc tatgcccgca ttcgcgagca gttcggcatt tccatatcca aattcgaagg
      841 catcgaggag ccgctggcgc gcatcgccgg cacggcctac ctgctcgacg gcgcgcggcg
      901 gctgacctgt gccgcgctga acgagggcca tcaccccgcg gtcatctccg gcatcatgaa
      961 gctgcaggcg accgaccgaa tgcggatcgt catcgatgac gccatggaca tccatggcgg
     1021 caaggccatt atagacggcc cgcagaatta tctcggcaat ctctaccgct cggtgccggt
     1081 cggcatcacg gtggaaggcg ccaacatcct gacgcgcaac ctcatcgtgt tcgggcaagg
     1141 ggcgatccgt gcgcatccct atctcctcga cgagatgaac gcgctccgcg atgacgatcg
     1201 cgagcgtggc ctcgatgcgt tcgatcgcgc gttctggaaa catgtcggcc acaccttcac
     1261 caatctgttc cgcgcctggg gtcggagctg gagctttggg ttgttcgcgc ccgcgcccga
     1321 cgcgagcgat gcgacgcgtt tctatcgtca gctctcacgc tacgccgcag gctttgcgct
     1381 cgccgccgat ttggcgctat tgactctcgg cggcgcgctc aagcgcaagg agatgctctc
     1441 ggcgcggttc ggcgacattc tttccgaact ctacctcttg tccggtgctc tcaagcgctg
     1501 gcaggacgag ggccggcaac gggacgattt cgcggcgctc gagtggtgca tggcgaacgg
     1561 tttccggacc atcgagagca ggcttgccga aatctgcgcc aatctgccga atcgcctcgt
     1621 cggcgctctt ctcaagtttt tcctgcagcc gttcggcgca cgggtgctcg gcccgtccga
     1681 tcgcgtggtg catcaatgcg cgcagcttgt gcttgagccc tcagcagcgc gcgaccgcct
     1741 cacgtctgat ctctcccatg tcgctgatga tggcggtcag gcgcgcctgg agaaggcgtt
     1801 cgtgctggtc gcctcgacgg aagagatttc gaagagactg cacgccgcac gcctgcgcga
     1861 tgtgaatgag gcggtgaaga agggcgtcat cacccagtcc gaagccgaga ggctcgcagc
     1921 cgcgcatgag gccgtcgcga aagtgatcga tgtcgacgat tttgccccag aggcactgtc
     1981 gccgatttac aggaagcccg ccgacgtgca tcagttcttc cagga
//
LOCUS       DATLJT010000525         2527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2788489,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000525 DATLJT010000000
VERSION     DATLJT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788489"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VM910_12145"
     CDS             complement(<1..391)
                     /locus_tag="VM910_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI63317.1"
                     /translation="MAGRFARGKFGLIAGLPDMGKGQIAAFIAAAVTAGVELPCDEGS
                     APQGNVIWFNAEDGARDTVLPRLVAAGADLKRIIFVNGARVGGVEKMFSLVTDLQLLR
                     AKIEEIGNVVLVIIDPVSAYLGVGKVDG"
     gene            complement(355..1512)
                     /locus_tag="VM910_12150"
     CDS             complement(355..1512)
                     /locus_tag="VM910_12150"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HVI63318.1"
                     /translation="MALRTEDPFLALPPLPHERLRIAVTARRIAYLKLGYEPIPILSG
                     RKRPAMNGWQDVPITIPPDEDVITPWADMYPGALSTGIRTRYTPGFDIDIKDQNVADQ
                     VEQALLNVVPSGTILKRVGQPPKRLIPFRCTTPFKKISATFKAPDDVVHKVEVLADGQ
                     QFVAEGIHETTQQPYRWADNVNLLNVAHAHLPLVEEELARRFVAEASEIMKRAGWVEV
                     GAQGKPKSNGKPNGKANGNTEIAAKTPGSSIYGRTALRSECDKLAAMPKDSGRNNALN
                     AAAFSLFQLVAGGELDEEKDQVAERLFSAAEACGLVADDGAASVRATIESGAKAGCAQ
                     ARRAPDGGGEQGDQTDEEAEDDDLVIAAADELEMCGVDWLWPAALPEASSG"
     gene            complement(1567..1800)
                     /locus_tag="VM910_12155"
     CDS             complement(1567..1800)
                     /locus_tag="VM910_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63319.1"
                     /translation="MNDIDDYDVFDGMRWLLLNKVDLDNQLAVERALWIPFYSAARPA
                     LKERFIKWCVQAARLNKESFGSAEYFVQAEDAP"
     gene            complement(1858..2211)
                     /locus_tag="VM910_12160"
     CDS             complement(1858..2211)
                     /locus_tag="VM910_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63320.1"
                     /translation="MRAAEAFPSKYLKSSDVKERPRIAVISHVAKEKVGKGPDAEEKH
                     VLHFEEGKPMVVNRTNWDSLEEAFGDSEDWPGHKVKIKCVRTQYQGKATDGIRVEPIV
                     AKPSLKNDLNDGIAF"
     gene            complement(2267..>2527)
                     /locus_tag="VM910_12165"
     CDS             complement(2267..>2527)
                     /locus_tag="VM910_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63321.1"
                     /translation="IGLKVKLDRPVDRERPCCLNICTIGPARGPHAGELICASCGQHR
                     GWLSKSTAQWIEHVATRFGAPTTPIVVRKAHTFEEEAPPTET"
BASE COUNT          408 a          787 c          786 g          546 t
ORIGIN      
        1 ggccgtccac cttaccgaca ccgagatagg cgctcaccgg gtcgatgatc acgagcacga
       61 cgttgccgat ctcctcgatc ttggcgcgca ggagctgcag gtcggtgacg aggctaaaca
      121 tcttctctac gccgcccaca cgagcgccgt tgacgaaaat gatgcgcttg agatcggcac
      181 cggccgcgac caggcgcggc aagacggtat cgcgcgcgcc gtcttcggcg ttgaaccaga
      241 tgacgttgcc ctgcggtgcg ctgccctcat cgcatggcag ctcgacccca gcggtcactg
      301 ctgctgcgat gaaggcggcg atctgcccct tgcccatgtc cggcaagccg gcgatcagcc
      361 cgaacttgcc tctggcaaag cggccggcca cagccaatcg acgccgcaca tttccaattc
      421 gtcagccgcg gcgatcacga ggtcgtcgtc ctcggcttcc tcatcggttt gatcgccctg
      481 ctcgccaccg ccgtcaggcg cccgacgtgc ttgcgcgcac ccagccttgg ctccgctctc
      541 gatcgtggcg cgcaccgagg ctgcgccgtc gtcggccacg agaccgcacg cttccgccgc
      601 ggagaacagg cgctcggcga cttgatcctt ttcttcgtcg agctcgccgc cggccacaag
      661 ctggaacagg ctgaacgcgg cagcattgag cgcattgttg cggccgctgt ccttgggcat
      721 ggcggcgagc ttgtcgcact cgctcctgag cgcggtgcgg ccgtagatgg agctgcccgg
      781 cgtcttggcg gcgatttctg tattgccatt ggccttgcca ttcggcttgc cgttgctctt
      841 gggcttaccc tgggcgccga cctcgaccca tcccgcgcgc ttcatgatct cgcttgcttc
      901 ggcgacgaag cggcgtgcta gctcctcctc cacaagcggc agatgcgcgt gcgcgacatt
      961 gaggaggttg acgttatcgg cccagcgata cggctgctgc gtggtttcgt ggatgccctc
     1021 ggcgacaaat tgctggccat cggccagcac ctcgaccttg tgcaccacgt cgtcgggcgc
     1081 tttgaacgtc gcgctgattt tcttgaacgg cgttgtgcaa cggaatggga tcagccgttt
     1141 gggtggctgg ccgacgcgct tgaggatcgt gccgctcgga accacgttga gcagtgcttg
     1201 ctctacctga tcggcgacgt tctgatcctt gatgtcgata tcgaaccccg gcgtatagcg
     1261 tgtacgaatg ccggtgctga gcgcgccggg atacatgtcc gcccagggcg tgatcacgtc
     1321 ttcgtccggc gggatggtta tgggcacatc ttgccagcca ttcattgccg gacgcttacg
     1381 gccggacagg atcgggatcg gctcgtagcc gagcttgaga taggctatgc ggcgcgccgt
     1441 gacggcgatc cgtaaccgct cgtggggaag cggcggtaag gccaaaaacg gatcttcggt
     1501 tcgcaatgcc atcgttgaag cttcaaaaaa tccactgagc gcgcaccttc ggtggcgagc
     1561 ttgcagttac ggcgcgtctt ctgcctgcac gaaatattcg gccgagccga agctttcttt
     1621 attcagcctg gccgcttgga cgcaccactt gatgaaccgt tccttgagcg ccggcctggc
     1681 agcgctatag aagggaatcc aaagggcgcg ctcgactgcc aattggttgt cgagatcgac
     1741 tttgtttaga agcagccacc gcatgccgtc gaacacgtcg taatcgtcga tgtcattcat
     1801 ttcgctctcc taatagagtg ccaccggacg ttgcggcgtc cggtggccga gttgttgtca
     1861 aaacgcgatc ccatcattga gatcgttctt cagcgacggc ttagccacga tcggttcaac
     1921 gcggatgccg tcggtggcct tgccttgata ctgggtccgg acgcacttga tcttcacctt
     1981 gtgcccgggc cagtcttcgg agtcgccgaa cgcttcctcg agcgagtccc agttggtgcg
     2041 attgaccacc atgggcttgc cctcttcgaa gtgtaggacg tgcttttctt cggcgtcggg
     2101 gcctttgccg actttttcct tcgcgacgtg cgagatcacc gcgatgcggg gacgctcctt
     2161 cacgtcggaa gacttcaggt actttgaagg gaaagcttca gctgcgcgca tcgcaaaatt
     2221 cctttcgtat gtgagcgcgg gttgaggttc gtgtcggtat tggggttcag gtttctgtgg
     2281 gcggcgcctc ctcttcaaac gtgtgggcct tgcgcacgac gatcggcgtg gttggcgcgc
     2341 cgaagcgcgt cgctacgtgc tcgatccatt gtgctgttga cttggacagc cagccgcggt
     2401 gctggccgca gctagcgcag atcagttcac cggcatgcgg cccgcgcgcc ggcccgatgg
     2461 tgcagatgtt cagacagcac ggccgctcgc gatcgacggg gcgatcgagc ttcaccttga
     2521 ggccgat
//
LOCUS       DATLJT010000526        10979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_260881,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000526 DATLJT010000000
VERSION     DATLJT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260881"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VM910_12170"
     CDS             <1..282
                     /locus_tag="VM910_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVI63322.1"
                     /translation="WSWFAMDTTTAVLFTLYMVPVNLLDNILRPLVAKGLTTPMPVIL
                     IGVLGGTLVHGMIGLFVGPIVLSIAWQLLVVWTRDGPKSVGRLEGSDGP"
     gene            1130..1375
                     /locus_tag="VM910_12175"
     CDS             1130..1375
                     /locus_tag="VM910_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63323.1"
                     /translation="MIGRILLSLIAFALTGFVVLTLIGLSDRYEQETAALGFSGIYER
                     YLASQAGFADNPKAYRAVVEAEHARAAGVGETAAFEE"
     gene            1714..2028
                     /locus_tag="VM910_12180"
     CDS             1714..2028
                     /locus_tag="VM910_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63324.1"
                     /translation="MPKNRGHIASLARSYTDTAIKTLAGIMMEQTSPPMARIAAAKVL
                     LDRGWGKPKEMHHSEVDDNKTLLKVVNEIVHVYETREQIEFNDQIPLLEINPSKNGGN
                     TQ"
     gene            complement(2222..2668)
                     /locus_tag="VM910_12185"
     CDS             complement(2222..2668)
                     /locus_tag="VM910_12185"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVI63325.1"
                     /translation="MKREKEAISGTVTGNDQHVGFRAMIMKQAIEYNLAGFTKNLPND
                     VVNFTLQGDAKRLTHALSAVHEGTKKSSDIKVNTTRGTVDPALNAFTIYAWTSTSRNI
                     TTPYDLVFHLRPDNGKLAPADVKTAWHGILQSTLKGDDLKKLRPDD"
     gene            3549..3749
                     /locus_tag="VM910_12190"
     CDS             3549..3749
                     /locus_tag="VM910_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVI63326.1"
                     /translation="MAGEIDKILWDVAIVGSGFAGALVANELSKGRRRVIILEAGEYI
                     PPNINRFESSRPSQLHAVLCVV"
     gene            4102..5922
                     /locus_tag="VM910_12195"
     CDS             4102..5922
                     /locus_tag="VM910_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63327.1"
                     /translation="MPWDDTSDDNPSSTPLQPGPVGEYLEVVDIDPASNKLYDPVDLN
                     NNQLLTQDGWPPSEGNPQFHQQMVYAVAMTTIEHFEKALGRKVLWAPRLGKTGNGSDE
                     DKRKLYDVRRLRLYPHALRTDNAYYSPDKKAILFGYFPSSSGRDDPTAPGSMVFACLS
                     SDIIAHEMTHALLDGLHRRYEEPSNLDVLAFHEGFADIVALFQHFTIEEMVRFEIDRV
                     RGNLSAEGLLGALAKQFGEGSGRRGPLRDYVKIDKNKLRYEDETEPHARGSILVYAVY
                     EAFRNIVARRTADLIRLATGGSGELPPGAIPPDLVNRLTVETCKVARHVLRMCIRALD
                     YCPPVDITFGEYLRALITSDLEMVPNDRYGYRVAFMEAFRKQDILPTDIRTVSEETLS
                     WNTLQGRKPTWLEDVIKSIAFTWNADLDRSEIFERNEQNRWSLWRQLKQLFQAHPEFY
                     ADFGLSPGVPRYHSDGTIKQERPTPDSTFEVYNVRPARRITPDGDLRTDIIAVIDQRR
                     AIPLDGKDVKNGFFWFRGGATLIIDPRKGKEEVRYTIVKNSDSESRQQRQRAMATGTA
                     APGMRTSLRALYFGGQVQGGGAPAEPFAMLHAGRGEYGND"
     gene            6035..7540
                     /locus_tag="VM910_12200"
     CDS             6035..7540
                     /locus_tag="VM910_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63328.1"
                     /translation="MAASAVAAGRPKVTIRFFCQGIGDCHLLKFPKDDGGDFWMLIDC
                     GIHSSITGGSEKIKAIVKNIRSLTTRLDVVVLTHEHWDHNSGFLTAKDEFQGIEVGEV
                     WMGWTEDPQDPQAQQLDKFKGAALDALQEANRRLGGVDGLGPHLSAVKNGLDAVLGFN
                     FGAKGEKVRSARNAAIELAKGRVTYLEPKKPPISLPALARCVRIYVLGPPRDAAMLGV
                     TEQASEMYGLGMESGWPIARALGNALSMSDSRGNQNLTAPFDSTEGHDLTLIRSADAR
                     ASSDAQLKKAVEFLDYFYDGRARPPQGNGKNERKPLDPNEADQAWRRIDHDWLGVSSD
                     LAIQLDDRTNNSSLVLAFEFLDTKRVMLFVGDAQIGNWLSWDKVSWMGDTTPTTAADL
                     LRRTVFYKVGHHGSHNATPKQHGLELMTSPHLSAFIPTNEQDAKKVKWGEMPFKPILE
                     ELEKRTSQRLIRADDPWLAQAAGKPSYGDSSGAILATRHAPDGLWVELDLA"
     gene            7965..9467
                     /locus_tag="VM910_12205"
     CDS             7965..9467
                     /locus_tag="VM910_12205"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HVI63329.1"
                     /translation="MSINFNPNDPQAGSTAPAMREKSPRANRPTSKSGFTFTGTSEEG
                     QFNPDTPQFLFWQCREAALAAVETWESVAGPHTRWQGNRNKLPLRQDAGLDLNAFYDR
                     TSFSFFHQAVGGKTFFSGASTDVVAHELGHGLLDSVRPDFFEVNFLEAGAFHEAFGDC
                     MAMLTALSDTETRQKLLAVSSDLRKRNFVETTAEDLSNGIKRMDPNHNAAEPRHAFNK
                     LKFQIPSTLPDDGGPGRLINEVHSFGMIFTGCFWDLIANLFGAASSKTEANLLNAARL
                     AGKILVAGAKGAVIAPRFLQSVGRAMVLADQSQNNGASVDHIRNAFQTHGILLGANAM
                     LAPSMALAGGAPKRASLTAATRRDLMERLGAGRRPKLSVRATNVFGTPAVEAVHAREV
                     PLGDIDKRLRGVVAIAHEPVLIGSSGGRASVMGAMPHGIDTEAEVRSFVNSLLAHGRI
                     ELGKPGKPKRAKGIVAGAGRIGGTTHRIKTVGGKKVLQRFRFLCGCCRCT"
     gene            complement(9657..10847)
                     /locus_tag="VM910_12210"
     CDS             complement(9657..10847)
                     /locus_tag="VM910_12210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI63330.1"
                     /translation="MMGRQKVDQCQLFYLFNLEGRIPTHHLLRRINSVVTRIMAELRG
                     NLASFPPAVEPQFAEEVAKTLDQAYRGLTRFDHETPYNDLGPWGVPRSRPMIRVVMFD
                     LGGTLVDNDRRPFPHITDALTAIASFKTADGKPLRSCLVSDYTMPEPPATQAKIAALF
                     KKYLAILDQTGLRAFFEPVKKRVTLSTHAGVAKPDRRIFETALQRLGAKVPLEACLFI
                     TEDAGHIKEAREKLKMQTLQFGSAGAGGFEDWADAPALVANLVAPGQAANSHAAIKAH
                     LAAKGVDLVHAEPAGSDRMTVSGQVWLPISVPGLKDLQHVHVAIPVQGEVTRGPKGEM
                     RSDVPTKPSNEQIAEATSYVRSLATHGQIAGHAAKHPQGATHAIETDQQGNRRLVRKR
                     FSAV"
BASE COUNT         2257 a         3319 c         3341 g         2062 t
ORIGIN      
        1 tggagttggt ttgcgatgga tacgacaaca gcggttctgt ttacgctcta catggtgccg
       61 gtaaatttgc tcgacaacat attgcgaccg ctcgtggcga aaggcctgac aacaccgatg
      121 ccggtaatat taatcggcgt cctcgggggc acgctcgtac acggcatgat tggtctattt
      181 gtcggtccaa ttgtgctttc gatagcttgg caattgcttg tggtttggac acgcgacggg
      241 ccgaaaagtg tgggccgtct agagggcagt gacgggccat gagcgtcatc aatctcgcca
      301 cccccgcagg cttccgcaaa gctcctcccg ggcgggccat aatcagcatt ccagaaccta
      361 ggcggctggt ctgacgatca cctgcagtat ccagctgaag tcctcaaccg tagcgatggc
      421 gtagcgagag tgaggaggag cagagtgagg aggagcagag tgaggaggag caggaggagc
      481 atggcctaaa tcctacgcca ggatcgagaa ctcggtcgaa cgccagtgct tccgtggcgc
      541 cggggtgggt ggttttcggc ggtttttctc cactccagtt ctgcttaacc gaaaagacac
      601 tgtcgtccgc taacgaagca cccatttggg gttggcagct agggcctggt catgagcatc
      661 gccagcttgg aatcgagtca aaaggcaacg gctatgagag aagtccttgc ctccaatgat
      721 gtccgcactc tcattgctaa ccagctaggc gtcgacgtca aacgtgtaac ggatgagcgc
      781 acttcaccga tgatctgggg gctgactggc tcgaccgcct ggaattaatg atcgtgatcg
      841 gagatcggtt cggggacgtc gtcatcacgg acgaggattc tgatccgtca catcgaaaac
      901 gtggacaatg agaggcggcg accctcgagg cgccgctcca gtgatccgca agctacggtc
      961 gcgtttggct ctgcgcgccg tggagcccgc tgcgcagcaa gaacgctgcg agcaggtaat
     1021 catccgcagc tggtcggccg acctatgtcg agtgggtccg gcctatttca cgcgaagacc
     1081 tttcgggcgt gattgtgacg gcaggggata actaaagagg ggggcaccaa tgattggtcg
     1141 cattctgctt agcttgatcg cctttgcgct caccggcttt gtggtcctta cgctgatcgg
     1201 tctctcggac cgttacgaac aggaaactgc agcattgggt ttcagcggga tttacgagcg
     1261 gtatttagcg tcgcaagccg gctttgcgga taatccaaag gcctatcggg cagtagtcga
     1321 agctgagcac gcgcgggcag cgggtgtggg agaaacggca gcgttcgagg aatgatgtcc
     1381 agcgaccagc ggaagccctc atgaccaagg attatctaat ggaagcgttt cgtgccagta
     1441 tcgatgcgga gcaaaagaag gctccaggac ggtgagatag atgatgcggt atggtccgta
     1501 ttcgaccggg ggaccgcagg ggccttagat cgagtcgccc agatagcggg gcgcaagtat
     1561 tgccgcccgc acgcgtgacc gttccgcctt ggcctcacgc gagtactatg attcaatgga
     1621 ggcagccttc agcgcgagct tggctacgac ttgattgatt tgtcagcgcc catgcggatt
     1681 gtaagaatgc ttacaaaaca tgacgagcgg aaaatgccaa aaaaccgcgg acacatcgcg
     1741 tcgcttgcgc gatcatacac cgacaccgcc atcaaaacgc tggccggaat catgatggag
     1801 cagacctccc cgccgatggc gcggattgcg gcggctaagg ttctgcttga tcgcggctgg
     1861 ggcaagccaa aggaaatgca tcacagcgag gttgacgaca acaagacgct gctcaaggtc
     1921 gtcaacgaga tcgttcatgt gtatgagacc cgcgagcaga tcgagttcaa cgaccaaatc
     1981 ccactgttag agatcaaccc gagcaaaaat ggcggcaata ctcaataacg gtcagccaaa
     2041 ggctggggat cgcgtgcggc tcgcccgcca tcccttcctc accggcaggg tcgacaagat
     2101 cgaattcggc gcggcgcata tgcgctggga cggctcgggc cttagcgcgc tccgttggga
     2161 gagccgtcca cgtcatcaga agccgaagtt cgcgccctcc acgataacca gacaacgctg
     2221 ctcaatcgtc ggggcgcagt ttcttcaggt cgtcgccttt cagagtcgat tgcaaaatgc
     2281 cgtgccatgc ggtcttgacg tcggccggcg cgagctttcc gttatcgggc cgcagatgga
     2341 aaacgagatc gtacggggtg gtgatgttcc ggctcgtcga cgtccaggcg tagattgtga
     2401 aagcgttcaa agccggatcg accgtccccc gagttgtgtt gaccttgatg tccgatgact
     2461 tcttcgttcc ctcgtgaacg gccgagagtg cgtgggtcaa tcgtttcgca tcgccctgaa
     2521 gggtgaagtt cacgacgtca ttgggcaggt tctttgtaaa gccggcgagg ttgtattcga
     2581 tggcctgctt catgatcatg gcccgaaatc ccacatgctg atcgttgccg gtgacagtcc
     2641 ccgaaatggc ttctttttcc cgtttcattg tcgtcctccg tcccttcgaa atcgccgaag
     2701 agttcgcgac ctcattgcga gctctgggtg cggtgtttgg cgcggagctt tcattcttag
     2761 aaagccccgg ccgtcgttca gcacggaacc ggcctccaat caaatgaaag tccgactagg
     2821 cttcaaggag ggccgaccaa accgaccacc acatcaccaa gtcgcaacta aaagtaggcc
     2881 agagccatgc gccccgagta tttcaggaga aaactttttt gcagaggggg ccccggtgcc
     2941 gccgcggatt gctgggcatt cgccccaact agcgaagaac ttgttcgcca gttaccttca
     3001 atggtatcaa gcacttagca cgatcgatgc ctgcggacgg cctcgaggca caagatgtag
     3061 tgccgggcca ttttcgtcgc ggcgatcggt ttttgggcaa agcacgcacg ctcgcgcgcc
     3121 cgccgaacca gcccgtttcc atatgtcact cccccttccc cctcgcgcgc cagcggcgct
     3181 gccgcagacg tgaactggat gctgatggcg atgatgtgcc cattccatgc gggtttcttg
     3241 aggtcagccg caggattagc aagcggctag caagcaaggt cgaccgcccg accgcgccgc
     3301 aaagcccagc atttgccggc attttcacat gcgcgggccc gcggcgcgca gggcctcata
     3361 aagagcgcgc cccgcgccgg cgcccatcgc gtcgagctcc tccggcgtgg agccacccat
     3421 ttcaatgagc ggaccactga cgacttgaag agcgcgacaa gcagtctctt ggccaaagcc
     3481 ggcaacaacc tcaagggctt cgtgctcgac ctacgcaaca ctccggggtt tcttaaggga
     3541 ggcagatcat ggccggggaa attgacaaaa tattatggga tgtcgcgatc gtcggctcgg
     3601 gctttgccgg cgccttagtc gctaacgaac tcagcaaagg ccgcagaagg gtcatcatcc
     3661 tcgaggcggg cgagtatatt cccccgaata tcaacaggtt cgaatcctcc cgccccagcc
     3721 agctccacgc tgttttgtgc gtagtgtgag ctagctcaca tcccggttcg gaagaaagga
     3781 acatccttct cgacggatgt gggagggcac gcgcgagcga tgcttcagct tcgctgacag
     3841 tgccggtccg atctgcaggt ctggtgaaac gaactggcat gaaaccaacc gctgatttcg
     3901 ggaggccgca tgttagagcc ttcgcaacgg tcaactgagc taggcacagt tggaagggcc
     3961 acagccagac gaggagccgc ggcggtaaaa tggaaaatcc ggaaacctcc ggccccgagg
     4021 cggcgcctgc gcgtctatgc ccttgatcca agcgtcggca agaccctcga ctcccttgcg
     4081 atcaacgaaa ctacgctctc cgtgccatgg gacgatacgt ccgacgacaa tccgtcctcg
     4141 acgccgcttc agccaggacc ggtaggggag tatctcgaag ttgtcgatat cgatcccgcg
     4201 tccaacaagc tctacgaccc ggtcgatctg aataacaacc aactactgac gcaggacgga
     4261 tggccccctt cggaaggaaa tccacaattc caccagcaaa tggtttacgc ggtggcgatg
     4321 acgaccatcg agcacttcga gaaggcgctc ggtcgcaagg ttctgtgggc gccgcgactt
     4381 gggaagaccg gcaacggaag cgatgaggac aaacgcaagc tatatgacgt caggcgattg
     4441 cgcttatacc cgcatgcgct gcgaaccgat aacgcctatt atagtcccga taagaaagcc
     4501 attttgttcg gctactttcc ttccagctcg ggtcgggacg acccaacagc gcccgggtcg
     4561 atggtgtttg cgtgtctttc gagcgacatc attgcccatg aaatgaccca tgcgctgctc
     4621 gatggactac acaggcgcta tgaggaaccc tccaacctcg atgttctcgc ttttcatgaa
     4681 gggttcgccg acatcgtggc gctgttccag catttcacca tcgaggagat ggtgcgtttc
     4741 gagatcgacc gggtgcgcgg gaatctctct gcagaaggat tgctcggcgc gctcgcgaag
     4801 cagttcggag aaggaagcgg tcggcgcggg cctctgcgcg actatgtcaa aatagacaag
     4861 aacaagctgc gctacgagga cgaaacggag ccgcatgccc gtggttcgat cctggtctat
     4921 gccgtgtacg aggcatttcg caacatcgtg gcgcgcagga ccgccgacct cattcgattg
     4981 gccaccggcg ggtccggcga gttgcccccc ggcgccatcc ctccagatct ggtcaaccga
     5041 ttgaccgtcg agacctgcaa ggtggcgcgt cacgttctgc gcatgtgcat ccgcgcgctc
     5101 gactattgtc ccccggtcga catcaccttc ggcgaatatc tgcgcgcact gatcacttcc
     5161 gacctggaga tggttccgaa cgaccgctat ggctatcgcg tcgccttcat ggaggcattc
     5221 cgcaagcagg acattctgcc gacggacatc cgcaccgtct ccgaggagac cctcagctgg
     5281 aacacccttc agggtcggaa gccgacctgg ctcgaggacg tcatcaaaag catcgccttc
     5341 acatggaatg ctgatctcga ccgctccgag atcttcgagc ggaacgagca aaacagatgg
     5401 agtctgtggc ggcagctcaa gcagctgttc caggcgcatc cggagtttta cgccgacttc
     5461 ggcctgtcgc ccggtgtgcc gcgataccat tcggacggca ccatcaaaca agagcggccg
     5521 acgcccgatt ccaccttcga agtgtacaac gtccggccgg cgcgccgtat cacgcccgac
     5581 ggggatcttc ggaccgacat cattgcggtg atcgatcagc gccgagcgat cccgctcgac
     5641 ggtaaggacg tgaagaacgg cttcttctgg ttccgcggcg gagcgacctt gatcatcgat
     5701 ccgcgtaaag ggaaggaaga ggttcgctac accatcgtca agaacagcga cagtgagtcg
     5761 cgtcagcaaa ggcaaagagc catggcgacg gggacggcag cgcccggcat gcgcacctcg
     5821 cttcgcgcgc tctacttcgg tggccaggtt cagggaggcg gcgcgccggc cgagccgttt
     5881 gccatgctgc atgccggccg cggagaatat ggcaatgact agttgcccaa acgtttcaaa
     5941 agcggcgtcg aagcgaggac agtccgggaa gcgggaagct tcaggagcag ctccggcgat
     6001 ggcgttggcc ggcgacgcag gtgagccgga gcccatggca gcaagcgccg tcgccgccgg
     6061 tcgaccgaaa gtcacgatcc gtttcttctg ccagggaatc ggcgactgtc acctgctcaa
     6121 gtttccaaag gacgacggag gcgatttctg gatgctgatc gattgcggaa tccacagctc
     6181 gatcaccggt gggagcgaaa agatcaaggc gatcgtcaag aatattcgct ccctcacgac
     6241 acgactggat gtggtcgtct tgacgcatga gcactgggat cacaattcgg gtttcctgac
     6301 ggcgaaggac gagttccaag gcatcgaggt cggcgaggtc tggatgggct ggacggaaga
     6361 tccgcaagat ccgcaagcgc agcagctcga caagttcaag ggcgccgcgc tcgacgctct
     6421 ccaggaagcg aaccggcggc tcggtggcgt cgacggactt ggcccacact tatcggcagt
     6481 caagaacggc ctggatgccg tgctaggatt caacttcggc gcaaagggcg aaaaagtccg
     6541 gtcggcgcgc aatgccgcga tcgagctggc aaaaggccgt gtcacatatc tggagccgaa
     6601 aaagccgccg atcagcctgc ccgcgcttgc aaggtgtgtg cgaatctacg ttctcggccc
     6661 tccgcgcgac gctgccatgc tcggcgttac cgagcaggcg tccgaaatgt acggccttgg
     6721 aatggagagc ggatggccta tcgctcgtgc gttgggcaac gcgcttagca tgagcgatag
     6781 tcgagggaat cagaatctca cggcgccgtt tgattctacg gaaggacacg acttaaccct
     6841 aattcgatcc gcggacgcca gagcgtcaag cgacgcccag ctgaaaaagg ccgtcgaatt
     6901 cctggattat ttttatgacg ggcgcgcaag gccgccgcaa ggaaacggaa agaatgaaag
     6961 aaagccgctc gaccccaatg aagcggatca ggcgtggcgc cgcatcgacc atgactggct
     7021 cggggtcagc tcagacctcg cgatccagct cgacgaccgc accaacaatt cgagtctcgt
     7081 tctcgcgttc gagtttctcg ataccaagcg cgtcatgctg ttcgtcggcg acgcgcagat
     7141 cggcaattgg ctcagttggg acaaagtgag ttggatgggc gataccacgc ccacgacagc
     7201 ggccgacctc ctgcggcgca ccgtcttcta caaagtcggc caccacggca gccacaacgc
     7261 gaccccgaag caacatggcc ttgaattgat gacgagccct cacctctctg cgttcatccc
     7321 gaccaatgaa caggacgcca agaaggtgaa atggggagaa atgccgttca agccgattct
     7381 tgaggagctg gaaaaacgca ccagccaacg tctcattcgt gccgatgatc cctggcttgc
     7441 gcaggccgcc ggcaaaccaa gttacggcga ttcatcaggc gcgatcttgg ccacgcggca
     7501 tgcgcctgac gggctgtggg tggaactgga cctcgcctga gccggatacc cgtgttcagc
     7561 gggaagatcc ctgcaaaagg tgtgtgcaga cgccggctcg cgcccgacat tcggccgcgg
     7621 tcgaccatga gcccgaaaag caggtagcag ttttcggaaa gatcatcctc tgacacaggg
     7681 gcagagcggc atgatgatgc aaagaaaaac cagacgctgt aagacgaaac gatgccgatg
     7741 cacgctcgcg cgtgaatttc acatctggtg tgagacgcct cacacatgcg cgtgccgcgc
     7801 acatgccacg atggcaacaa tgtggcaaca ataccgtgta cccagagccg ggacgcatcc
     7861 cgcctcatcc actcggcttt cgcggtcccc accaccgcgt tcgggctcat gttttcgcaa
     7921 gcgatcccat ccttcgaccg ccagattcac gaggaggaac caccatgtcg atcaatttca
     7981 atcccaacga tccgcaagcc ggatccaccg cgccagccat gcgggaaaag agcccgcggg
     8041 cgaaccggcc gacgtcgaaa tccgggttca cgttcactgg cacgagcgag gaggggcagt
     8101 tcaaccccga cacgccgcag ttcctgttct ggcagtgccg cgaggcggcg ctggcggcag
     8161 tcgaaacctg ggaaagcgtc gccggcccgc atacgcgctg gcagggcaac cgcaacaagc
     8221 tgccgctgcg ccaggacgcc ggcctggatc tcaatgcctt ctatgaccgt acaagctttt
     8281 cgttcttcca tcaggcagtg ggcggcaaga cgttcttttc cggcgcgagc accgacgtgg
     8341 tcgcgcacga actcggtcac ggcctgctcg actccgtgcg ccccgacttc ttcgaggtca
     8401 attttctcga agcgggcgct tttcacgagg ccttcggcga ctgcatggcg atgctgacgg
     8461 cgctcagcga caccgagacg cggcagaagc tgctcgccgt cagcagcgac ttgcgcaagc
     8521 gcaacttcgt ggagaccacg gcggaggacc tctccaacgg catcaagcgc atggacccga
     8581 accacaatgc cgccgagccg cgtcacgcct tcaacaagct taagttccag attccctcca
     8641 cgcttcccga cgacggaggt cctggccggc tcatcaatga ggtgcacagc ttcggcatga
     8701 tcttcaccgg ctgcttctgg gatctgatcg ccaatctgtt tggggcggcg tcgtcgaaga
     8761 ccgaggccaa cctcctcaac gccgctcgcc tcgccggcaa gatcctagtc gcaggggcca
     8821 agggcgcggt gattgcgccg cgcttcttgc agtcggtcgg ccgcgccatg gtcctcgccg
     8881 accagtcgca gaacaatggt gccagcgtcg accacatccg caacgcgttc cagacccacg
     8941 gcatcctgct cggcgccaat gccatgctgg ccccttcgat ggccctcgcc ggcggtgcgc
     9001 ccaagcgcgc ctccctgacg gcagcgaccc ggcgcgacct catggagcgc ctgggcgccg
     9061 ggcgccgccc caagctcagc gtacgggcga ccaacgtctt cggaaccccg gcggtggagg
     9121 cggtccacgc ccgcgaggtt ccgctcggcg acatcgacaa gcgtctcagg ggcgtcgtgg
     9181 cgatcgccca cgagccggtg ctcatcggtt cgtcgggcgg tcgcgcctcc gtcatgggcg
     9241 ccatgccgca cggcatcgat accgaggccg aggtccgctc gttcgtgaac tcgctactgg
     9301 cgcacggccg catcgaactc ggcaagccag gcaagccgaa gcgggcaaag ggcatcgtgg
     9361 caggggccgg acgcatcggc ggcactaccc accggatcaa aacggtgggc ggcaagaagg
     9421 tgctgcagcg cttccgcttc ctgtgcggat gctgccggtg cacttgagcg cagcgacgcg
     9481 gtgcgccccg gcctgtccgg ctcgcgatga cggggatcct ttttgaggca cgtgctttcc
     9541 ggttgtcatc ccggacggct cgttacgcca gccgatccga gcccgcgata gagcccgtcg
     9601 caactcaggg cgtcctggat cctagctctc agcgcggcgc tgggttccgg tgcgattcag
     9661 acggccgaga agcgcttgcg cacgaggcgg cggttgcctt gctggtcggt ctcgatcgca
     9721 tgggtggcgc cctgagggtg cttggcggcg tgcccggcga tttggccgtg ggtcgctagg
     9781 ctgcgaacgt aggaggtcgc ctcggcgatc tgctcgttgg acggcttggt cggcacgtcc
     9841 gaacgcattt cgcctttcgg tccgcgcgtg acctcgccct gcaccggaat cgccacgtgg
     9901 acatgctgca gatctttgag tcccggcacc gagatcggca accacacctg tccggaaacg
     9961 gtcatccggt cggatcctgc tggctccgca tgcacgaggt cgacgccttt tgccgctaga
    10021 tgcgccttga tggcggcatg gctgttcgcc gcttggccgg gtgccaccag gttggcgacc
    10081 agggccgggg catcggccca gtcctcgaac ccgcccgctc ccgcgctgcc gaactgcagc
    10141 gtctgcatct tcagcttttc gcgcgcctcc ttgatgtggc ctgcgtcttc cgtgatgaac
    10201 aggcacgcct cgagcggcac cttggcgccg aggcgctgca gcgccgtttc gaagatcctg
    10261 cggtccggct tagcgacgcc cgcatgcgtc gacaacgtca cgcgcttctt caccggttcg
    10321 aagaacgcac gcagcccggt ctggtccagg atcgccaggt attttttgaa cagcgcggcg
    10381 atcttggctt gcgtcgccgg cggctccggc atggtgtagt cggagacgag gcaggaacgc
    10441 aggggcttgc cgtcggcggt cttgaacgac gcaatggccg taagggcgtc cgtgatgtga
    10501 ggaaatgggc gacgatcatt atcgacgagc gtcccaccga gatcgaacat caccacgcgt
    10561 atcatcgggc ggctccgcgg cacgccccac ggcccaaggt cattataggg ggtttcatga
    10621 tcgaagcgag tcaaaccgcg gtatgcctgg tcgagcgttt ttgctacctc ttcggcgaac
    10681 tggggttcaa cagcaggagg aaatgatgcc aagttcccgc gaagctccgc cattatccgc
    10741 gtcaccaccg aattgatgcg gcgaagaaga tggtgcgtcg gaatgcgacc ttcgaggttg
    10801 aacagataga acagctggca ctgatcgacc ttctgccgac ccatcatgat gtgccccgtc
    10861 ctgaaaactt caaggacacg gaatcatgat tcgtcgatat tcacgaggac tttttcaaca
    10921 gtatcgaccc atctcggacc taagcggcgc aggaatggtg cattgcactc tcgccctct
//
LOCUS       DATLJT010000527         7115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1868735,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000527 DATLJT010000000
VERSION     DATLJT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868735"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VM910_12215"
     CDS             complement(<1..702)
                     /locus_tag="VM910_12215"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI63331.1"
                     /translation="MGDFHGVFPYLVSPIDASGKIREAVLARLCDDLIKSGVHGLTPL
                     GSTGEFAYLDSEQRMAVVRTTIEAARRRVPVVAGVVSTSTADAVAQAKAYQKLGADGI
                     LAILEAYFPLPDGGVESYFRAIADAVDIPVVIYTNPQFQRSDLTLDVIARLSSHPRIA
                     YIKDASTNTGRLLSIINRCGDDIRVFSASAHIPAAVMLIGGVGWMAGPACIIPRQSVE
                     LYNLCKRQRWEDAMAL"
     gene            complement(875..1141)
                     /locus_tag="VM910_12220"
     CDS             complement(875..1141)
                     /locus_tag="VM910_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63332.1"
                     /translation="MKKLVLTVAAVAALAAAASAPAEARIMRVRPTGIAMAGGASTAA
                     AAATAAAIAADAYLYGPYGYYVGPAAPVYYGYAAPVYPGYGYRF"
     gene            complement(1305..2945)
                     /locus_tag="VM910_12225"
     CDS             complement(1305..2945)
                     /locus_tag="VM910_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI63333.1"
                     /translation="MPVAYPPPVVLWSWTGLYIGGHVGAAVGNATITDPAGPAIYGNN
                     VRSTGVLGGVQIGYNWQVPNSLFVLGAEADVAALGSFGSATCLASSGFFISANCRVRP
                     DAVGTATGRVGFAAGPFGRTLLYVKGGLAWLDDRIDITSNALAPPQVTSFDGARFGWT
                     AGAGIERALTPAWSLRLEYDYMRFGDLSMATPASLFQVVPPSLFGFVQTNGGTASISQ
                     NLQTVKLGLNYKIGEDLHAHWEPSASDYHLRGTTDAGYVPDADVEIGARVWYSSGRFQ
                     KDLGATVDPTQSSNLISRLTYNTTAATGEVFGRVDSPSNIFLKGFLGGGAITSGNLHD
                     EDWAAPIVPGQIVPYSNTVSTVNGDLAYGTIDAGYSVFRGPSTNVGGFIGFNYYRENK
                     KAYGCNQIANPFSDCVPALPNSILGITEDDRWYSFRIGVNGVVKLFDRLKLAADVAWL
                     PFASFSGTDNHLLRTDVVNTVSTESGSGQGVQLETILSYAFDNGFSIGAGGRYWAMWA
                     PAAYTHDFGAPCPCLTLPVKTERYGAFLQASYKLGGPY"
     gene            2944..3069
                     /locus_tag="VM910_12230"
     CDS             2944..3069
                     /locus_tag="VM910_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63334.1"
                     /translation="MSAASAKPELKPTTKAAANIFFTTQPELVSGISLTKIGRNA"
     gene            3321..3671
                     /locus_tag="VM910_12235"
     CDS             3321..3671
                     /locus_tag="VM910_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63335.1"
                     /translation="MARIARRGSAPLPIHAAIVALLALAGCAQSSAPELIQNASTEDA
                     FACKLEPALLVPQSAPDCVFRRAELKTIDPDQWARLKIEYERQCYQNAEKTVRERLRR
                     LQAANRCESKQAFR"
     gene            3845..4876
                     /locus_tag="VM910_12240"
     CDS             3845..4876
                     /locus_tag="VM910_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HVI63336.1"
                     /translation="METLYDLLGALPRDDAEGLRVAFRRAVKGAHPDLNPGDPDAGLK
                     FRQIVRANEILGDTDQRAAYDHLLDLAHIEQRQSAKRAVAHTIYQITSGVISFCVALT
                     VIAGGYLAVMQFPDEWTVPLAQVAAAVRQSTEAAAAAVATVRQSSEAAATIVRQVTEP
                     VTAAVSQPPVAAPEVTASTDLQAGAAPDKPDRIDLPPEPVLPTAVTLQTDIGGPPTVT
                     IGPPLDLTLLDAKSYREQGIVAYRKGDLNGAIADFDQAIQLDPKFAAAYIDRGIVFYR
                     LRKFDRAFADIAHAKRIERSGRTRPSPQSVVKKPRPPVELSPAPPPRRQTANLDPSRE
                     EGFASTRRP"
     gene            5340..5957
                     /locus_tag="VM910_12245"
     CDS             5340..5957
                     /locus_tag="VM910_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI63337.1"
                     /translation="MDDLTEVPSDKPVLFIVGLGANQAVTCSKIRNQYPLASIVAVAD
                     ESNPRFLASALDDGANAALFSSVSPTALVNTLQTVINGKLILIDARLWSLEIQPKTEE
                     QRMSPPLQNVIPWEVTDEPITTKQLSAREIAILERIVRGDSNKHVARFFKIAEPTVKA
                     HVKAIFRKIGASNRTQAAIWALNHRLFDDAGPNDLPLLLQNDSGR"
     gene            complement(6001..6156)
                     /locus_tag="VM910_12250"
     CDS             complement(6001..6156)
                     /locus_tag="VM910_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63338.1"
                     /translation="MTMRHFALFVSLVLATVSVVEVATGSKKAALGPAGRRGASGRTQ
                     LAEPGLF"
     gene            complement(6576..6812)
                     /locus_tag="VM910_12255"
     CDS             complement(6576..6812)
                     /locus_tag="VM910_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63339.1"
                     /translation="MIAYWAVPYLLVAAPPVILLLTILMGGCTMTRTASDPMDSGARF
                     YSDPNSQASGELIKELDRRAAREDLRRAVEEIQR"
     gene            complement(6809..>7115)
                     /locus_tag="VM910_12260"
     CDS             complement(6809..>7115)
                     /locus_tag="VM910_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63340.1"
                     /translation="EDVTEIGKLSAEAIVKEYEATAKEIEAMGEFVKEMVQRCEQLTA
                     SSAAMLKDIKLTAMRYRKEGKRMFNEIESCSTTTDEVRKLCESFRDRVATKPTEGAT"
BASE COUNT         1421 a         2191 c         2208 g         1295 t
ORIGIN      
        1 gagcgccatc gcatcctccc agcgttgccg cttgcagaga ttgtagagtt cgacgctttg
       61 cctggggatg atgcaggcag gtcccgccat ccagccgacg ccgccgatca gcatcacggc
      121 ggccggaata tgggcggacg cggagaacac gcggatgtcg tcgccgcaac gattgatgat
      181 cgagagcagc cgcccggtat tggtggaggc atccttgatg taggcgatgc gcggatgaga
      241 cgagagccgc gcgatcacgt cgagcgtgag atccgagcgc tggaattgcg gattggtgta
      301 gatcacgacg gggatatcga cggcatccgc aatggcgcgg aaataggatt cgacgccgcc
      361 gtcgggcagc ggaaaataag cctcgagaat cgccaaaatc ccgtcggcgc cgagcttttg
      421 ataggccttt gcctgcgcca cggcatccgc agtcgaggtc gagaccacgc cggccacgac
      481 cggtacgcgc cgacgtgcgg cttcgatcgt ggtgcgcacc acggccatgc gctgctcgct
      541 gtcgagatat gcaaactcgc cggtcgagcc gagcggggtc aggccatgca cgccggactt
      601 gatcaggtcg tcgcaaagcc gtgccagcac ggcctcacgg atcttgccgg acgcgtcgat
      661 cggggaaacg agataaggga agacgccgtg gaaatcgccc atgatgtgcg ctgatatagc
      721 gggcgcaaca cgcgacgcgc aagcacgtca gggataggga ttgccgaacg cgttcgaagc
      781 aaagcatggc ccgcggcacc ggtggtgcga ttctgacgtt cggtagcgaa cgtcggatcc
      841 gctccaccag ccgtgcgagc catccgccct gtcatcaaaa gcggtagcca tacccgggat
      901 agaccggcgc agcgtagccg taatacacag gcgccgccgg accgacgtaa tagccatagg
      961 gtccgtagag ataggcgtcg gctgcgatcg cggccgcggt cgcagcggcg gctgccgtcg
     1021 aagcgccgcc ggccatggca atgccggtcg gcctgacgcg catgatgcgc gcttccgcgg
     1081 gcgcgctcgc agcagccgcc agcgcggcga cggcagcgac agtcagaaca agctttttca
     1141 tccgaatatc ctttccttca atcatctggg gacgagtcga ggtggagtga cggatcgaat
     1201 ccgtcacccg tccggccaga gtatgaaggg cgggcttgcg gttatgtgcg cggcgtcaca
     1261 ttcgcagaac gcatgggacg gcggaacggc gctgtgaaag agcgctaata cggcccgccg
     1321 agcttgtagg acgcctgcag gaaggcgccg taacgctcgg tcttgaccgg caaggtcaga
     1381 cagggacatg gcgccccgaa gtcgtgagta taggcggccg gcgcccacat cgcccagtag
     1441 cggccgccag cgccgatgct gaaaccgttg tcgaaggcat aggacaggat ggtttcgagc
     1501 tgcacgccct gcccgctgcc ggattcggtc gagacggtgt tgacgacatc ggtgcggagc
     1561 aggtggttgt cggtgccgct aaacgaggcg aacggcagcc aggcgacatc cgccgccagc
     1621 ttgagccgat cgaacagctt gaccacgccg ttcacgccga tccggaacga ataccatctg
     1681 tcgtcttcgg tgatgccgag aatggaattg ggaagggcgg gcacgcagtc cgaaaacgga
     1741 ttggcgatct ggttgcagcc atacgccttc ttgttctcgc ggtaatagtt gaagccgatg
     1801 aagccgccga cattggtgct cggtccgcga aacaccgaat agcccgcgtc gatcgtgccg
     1861 taggcaaggt cgccgttcac ggtcgacacc gtgttcgaat aggggacgat ttgcccggga
     1921 acgatgggtg cggcccagtc ctcgtcatgc agattgcccg atgtgatcgc cccgccgccg
     1981 agaaaaccct tcaggaagat gttcgaaggg ctgtcgacac ggccgaacac ttcgcccgtc
     2041 gccgcggtcg tattgtaggt gagccgggaa atcagattgc tggattgcgt cggatcgacg
     2101 gttgcgccga gatccttctg gaagcggccg gacgagtacc agacccgcgc gccgatctcg
     2161 acgtccgcat ccggaacata gccggcatcg gtggtgccgc gcagatgata gtccgatgcg
     2221 gacggctccc aatgcgcgtg gagatcctcg ccgatcttgt agttcaaacc gagtttgacg
     2281 gtttgcagat tttggctgat cgaagccgtg ccgccgttgg tctgaacgaa gccgaagagc
     2341 gatggcggaa cgacctgaaa gaggctcgcg ggcgtcgcca tgctgaggtc gccgaaccgc
     2401 atatagtcgt attcgagccg gagcgaccag gccggagtga gcgcccgctc gatgccggcg
     2461 cctgcggtcc agccaaatcg cgcaccatcg aagctcgtca cctggggcgg cgcaagcgcg
     2521 ttggaggtaa tgtcgatccg gtcgtcgagc caggcgaggc cgcccttcac atagagcagc
     2581 gtgcgtccaa acggtcctgc cgcgaagccg acgcggccgg tggcggtacc gacggcgtcc
     2641 gggcgcacac ggcaattggc ggagatgaag aagccggagg aggcaaggca ggtggctgag
     2701 ccaaagctgc cgagcgcggc gacgtcagcc tctgcgccga gcacaaaaag actgttgggt
     2761 acctgccagt tgtaaccgat ctgcacgccg cccagcacgc ccgtgcttct gacgttgttg
     2821 ccgtagatcg cgggaccggc gggatcggtg atcgtcgcgt tgccgacggc ggcgccgaca
     2881 tggccgccga tataaagccc ggtccacgac cacagcacga ccgggggcgg atacgcgacc
     2941 ggcatgtccg ccgcctccgc gaaaccagaa ctcaaaccaa cgacaaaggc cgccgccaat
     3001 atcttcttca cgacacaacc cgaacttgtt tccggcattt cattaaccaa gatcggtcga
     3061 aatgcttaag aaaggctgag cggtgaggat tggctaacca tgaattttaa gtcggccttc
     3121 agcatgaagg cgcggccgca cgcgaaggtt cgtctgttca gaggccgtta agcttaattg
     3181 ttaaatttcg attcacgctt tgcttccatg atcgccccgc ttggacggcg gcccaggcgt
     3241 cattgcgcaa gacgcaaatt gcgatgtgcg cgacgcgagg aagggccgac caggcgatgc
     3301 gcgagacatg ttccgaagct ttggcgcgga tcgcgcgccg cggttccgcg ccgttgccaa
     3361 tccatgccgc gatcgtcgcc ctcctcgcgc ttgcgggatg cgcgcagagt tcggctccgg
     3421 agctgatcca gaacgcttcc acggaggatg cgttcgcctg caagctggaa cccgccttgc
     3481 ttgtgccgca atcggcgccg gattgcgtgt tccgccgggc ggagctgaaa accatcgatc
     3541 ccgatcaatg ggcccggctg aaaattgaat atgagcggca atgctaccaa aacgccgaga
     3601 agaccgtccg cgaacggttg cgccggctgc aggcggccaa ccgatgtgag agcaagcagg
     3661 cgttccgctg acccccggtg caagtgcgct gctgcgcggt cgcgcgtgta agttgcactt
     3721 ttcaggtcat ctttttgccg tgctgcaaca gcatccttca ccccatcagc gcatgtttct
     3781 ccacgcgaat tcgcggcggg aatttgaatg gagaagatag agttgtgtag ccgcgagtgc
     3841 gacgatggag acactgtacg atttgctcgg tgcgcttcct cgtgatgatg ccgaaggatt
     3901 gagagtcgca tttcggcgcg ccgtcaaagg cgcgcatccc gacctcaatc ccggtgatcc
     3961 cgacgccggc ctcaaattca gacagattgt tcgcgccaac gagatcctcg gcgacacgga
     4021 tcagcgtgcc gcctacgatc atctgctcga cctcgctcat atcgagcagc ggcagtcggc
     4081 gaagcgcgcg gtcgcccaca cgatctacca gatcacgtca ggcgtgatct cgttctgcgt
     4141 cgcgctcaca gtcatcgcgg gcggctatct tgccgtcatg cagtttccgg atgagtggac
     4201 tgtgccgctt gcccaggttg ccgccgccgt gcggcaatcg acggaagcgg cggccgccgc
     4261 ggtcgccacg gtgcgccagt cgtcggaagc ggcagcaact atcgtgcgcc aagtgacgga
     4321 gcccgtgacc gcggccgtga gccaaccgcc ggttgccgct cccgaagtaa ccgcaagcac
     4381 ggatttgcag gccggcgcgg ccccggataa gcccgaccgc atcgacctgc cgccggagcc
     4441 ggtcctgccg acggccgtaa cgctgcaaac cgacatcggc ggaccgccga cggtaacaat
     4501 cggaccgccg ctcgacctca ctttgctgga cgcgaaatcc tatcgcgagc aaggcatcgt
     4561 cgcctatcgc aagggcgatc tgaacggcgc gattgccgat ttcgatcagg cgatccagct
     4621 cgatccgaaa ttcgccgccg cctatatcga tcgcggcatc gtcttctatc gcctgcgcaa
     4681 attcgaccgc gcctttgccg acatcgccca cgccaagcgc attgaaaggt cgggtcggac
     4741 caggccttcg ccgcaatccg tcgtcaagaa gccccgtccc ccggtcgaac tgagcccggc
     4801 cccgccgcca aggcggcaaa ccgccaatct cgacccctcg cgcgaggagg ggtttgcttc
     4861 caccaggcgg ccgtaaagcc cccaaaagcg cccaaaaggt cgacttttgg cctgctcaag
     4921 aggcgtaacc ccttcgaagt acctcctccg gaccacccaa cggctgaacc gacctgagtc
     4981 ccgtgtttgc cggggactca aggggtgaaa gcagtcattt ttgtcatttc gggttgctgg
     5041 aactcttgcg agttcccctt acttttcccg ggttccccta cagcactatt tccgggttcc
     5101 agccaaaaat gtcctaggtt aattgccaaa tacgccattg gttgtatgtt ccaaagggta
     5161 tggaggaaag gggcaggcta cgaagatgcg gtgaccgttg taattggagc ggctactaaa
     5221 gcatgtcgag tgagcccgtt gtgcccacat atatcctcca gagtgatggc ctgttccgcg
     5281 aaggcctgcg actcatactt tcggccaccc gctttcggcc tcacggttgc gcgatcgaat
     5341 tggacgatct caccgaggtc cccagcgaca agccggtttt gttcatcgtc ggactaggtg
     5401 cgaaccaggc tgtcacttgc agcaagattc gcaaccaata tccgttggcc tctatcgtgg
     5461 ccgtcgccga tgaaagcaac ccgcgcttcc tcgcaagcgc gctggatgac ggagcgaacg
     5521 cagctctctt cagttcggtc agcccgaccg ctctcgttaa cacgcttcaa acggtgataa
     5581 acggaaagct cattctcatc gatgcccgtc tttggtcgct cgagatccag ccgaagaccg
     5641 aagagcagcg gatgtcgcct ccgcttcaga acgtgatccc ttgggaagtg acggacgagc
     5701 ccatcaccac caaacaattg tccgcgcgcg agatcgcaat acttgaacgt atcgttcgag
     5761 gagattccaa caagcatgtt gcgcgattct tcaagatcgc ggagccgacc gtgaaggctc
     5821 atgtgaaggc catcttcagg aagatcggcg cgagcaaccg cacccaggct gcgatctggg
     5881 cgctgaacca caggctgttc gatgatgccg gaccgaacga tctgcccttg ttgctccaaa
     5941 acgactcagg tcgctgagga gcgccagatc gactccgcct cacggcgagc cagcgaaaat
     6001 ctagaataat ccaggctctg caagctgagt gcgtccgctc gccccgcggc ggccggcggg
     6061 accgagcgcg gccttcttgc tgccggttgc gacctccacc acggacacgg ttgccagcac
     6121 gagcgagacg aacagcgcga agtggcgcat ggtcacgagc ggctttgccg gaggcacagg
     6181 ctccgggttg agctctgact ccggctcgag ctcgtctctg gcgcgaagat cgacatctcc
     6241 ggacggggat gatggtcata ggtccagcat ccgtcgtggt ctttgacaag cacccgcgtt
     6301 ggccaggacc gtgccgcctc gccgtggtcg aggcaatgat gagtggcgta agctgctgag
     6361 gatagcgtaa gcgccagaaa aactatcgcc gccgaaagca gcggaagacc atgcctgagg
     6421 cggacatagt ttccaatgat catcgcatcg gtggagctga attgaaccgt tatcggggtc
     6481 gtaccattga gcgaaacatt cccggcaaat ctttgcatcg ccatgccaga acgatgcttt
     6541 gacgtattcg acgtcgcgcc ggcaacaatc gcagatcatc gctgtatctc ctcgacggcg
     6601 cgccgcaggt cttcgcgcgc cgcacgacgg tccagctctt tgatcagctc cccactcgcc
     6661 tggctgttcg ggtcggaata aaaccgtgca ccactatcca tcggatcgct ggcggttctt
     6721 gtcatcgtgc agccgcccat caggatggtg agaagcaaga tgacgggcgg cgccgcgacg
     6781 aggagatacg gcaccgccca gtaggcgatc acgtcgctcc ctcggtgggt ttggtcgcga
     6841 cccggtcccg aaacgactcg cagagcttgc ggacttcgtc cgtagtggtc gagcaagact
     6901 cgatctcgtt gaacatgcgc ttgccttcct tgcgatagcg catcgccgtc agcttgatat
     6961 ctttgagcat ggcagccgag gacgcggtga gctgctcgca gcgctgcacc atctctttca
     7021 cgaactcgcc cattgcttcg atctccttgg cggtcgcctc gtattccttg acgatcgcct
     7081 cggccgaaag cttgccgatc tccgttacgt cttcg
//
LOCUS       DATLJT010000528         3522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3017708,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000528 DATLJT010000000
VERSION     DATLJT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017708"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..351)
                     /locus_tag="VM910_12265"
     CDS             complement(130..351)
                     /locus_tag="VM910_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63341.1"
                     /translation="MNRADELVHDAVSLLRDRGFVPTVSNGGRHLRIRWFDSGRRYTL
                     YIPSTPSDYRARLNSRATLRRMLRNNGER"
     gene            complement(348..704)
                     /locus_tag="VM910_12270"
     CDS             complement(348..704)
                     /locus_tag="VM910_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63342.1"
                     /translation="MLQVTSRRSLAKTRRRAKARGFRVLNDGCGCYSGIDTKIEPPRP
                     LVGLDHVPLWAVEQILLTPLPEPPPRRERTARPVKPTPTVEAVAPVVAEAPPHAQAHH
                     SFLALVEALKARGGAS"
     gene            complement(799..1020)
                     /locus_tag="VM910_12275"
     CDS             complement(799..1020)
                     /locus_tag="VM910_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63343.1"
                     /translation="MKKKRHIPIPADVAGLSPLELARKIPVAEAAKFNGVHVQTFLKR
                     YRHLVRRIGQRRLFVTVHDAIMLPPPDTS"
     gene            complement(1210..1914)
                     /locus_tag="VM910_12280"
     CDS             complement(1210..1914)
                     /locus_tag="VM910_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63344.1"
                     /translation="MAGTNGTDNDNSTFRLLCDEYALFLRLTRSSKKFANKLVQRFLE
                     EGERDRDGRIRYKLWLREVWPGGVVPPSPYDGDFWRSDPGRDINCTIEPWNGFARWTA
                     PASAEWREFDGREVADHQVFGIRLNHDVVLDFLESIGLLSKQAQSGEPSTAEGAPEES
                     LSKSAPVKSAPLSEKNITLKSWVPRALVDHPPPRGEKDIAGHLMNFAPKPWKKHSIQN
                     VLSGSAPEEKPKPKKS"
     gene            complement(1984..3219)
                     /locus_tag="VM910_12285"
     CDS             complement(1984..3219)
                     /locus_tag="VM910_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63345.1"
                     /translation="MMRLTAATIRALKLPPGATDRVYFDEDLPGFGLRVRASGVHSWM
                     IQYAIAGRTRRVVLGLASALDPGKARATAKDLLAQVRLGRDPAAEKDRARVRAAETFG
                     ALLPRFLERQRTRLKPRSYVETERHLQRHAKALHGSPIEAVARRTVAARLAEIEKHNG
                     PAARNRVRASLSAYFTWAAKEGYVDNNPVAFTNKAEEKARERVLSDEELRTIWLAADD
                     SQFGAIVRLLMLTGARRSEIGGLTYGEISPTLPLVTLPSTRTKKGREHMIPLSEPALS
                     ILKTLPKRTNADGTPREHVFGTIVGCGYQNWSRGKVDLDARIAASHGKALEWRLHDFR
                     RSVSTALHDRLGIPPHVVEEILGHVGGHKGGVAGTYNKAIYLEERRRALDLWGAHIME
                     LVTGKSAKGRVVDLHGRRR"
BASE COUNT          657 a         1051 c         1094 g          720 t
ORIGIN      
        1 aggtcgcgcc aaaggggccg gtatttgcga agtacgcttg accgcaatgc gtgtcgcgaa
       61 tctgctgctc gagcgtgtcc gaataaccgt gtgttagata cgacgaggac gattggggca
      121 ggcgcaccat tagcgctctc cattgttgcg cagcatccgt cgcagcgttg ctcgtgaatt
      181 gaggcgggcg cgatagtcac tgggcgtgct cggaatgtag agggtgtaac gtcgaccgct
      241 gtcgaaccaa cgtatgcgca ggtgtcggcc accattcgag acggtgggga cgaagccgcg
      301 atcccgaagc agggagacgg cgtcgtggac gagctcgtcg gcgcggttca tgacgcgcct
      361 cctcgtgcct tgagggcttc gaccagggcg aggaacgaat ggtgtgcctg ggcgtggggc
      421 ggggcttccg ccacgactgg cgcaaccgcc tcgactgtcg gcgtgggctt gaccgggcgt
      481 gccgtccgct cccggcgtgg cgggggctcc ggcaatggcg tcaggaggat ctgctcgacc
      541 gcccacaggg ggacgtgatc gaggccgacc agcgggcgcg gcggctcgat cttggtgtcg
      601 atcccggaat agcagccgca tccatcgttg aggacgcgga agccgcgagc tttggcccgt
      661 cggcgggtct tggcaaggga cctgcgggat gttacttgca acatgggaaa gttctcccgg
      721 tctcggaagc gttggaagcg ctcccgcgac ggttcagctt cagattcggg tggcagtcgg
      781 cggccttcgg gtcgccggct agctagtgtc cggcggcggc aacatgatcg cgtcgtggac
      841 cgtcacgaac aggcgccgct gaccgatccg gcgcaccaga tgtcggtagc gctttaagaa
      901 agtctggacg tgcacgccgt taaacttcgc ggcttcggcc actggtatct tgcgagcaag
      961 ctccagtggg ctaagaccgg cgacgtcagc gggaatgggg atatggcgct tcttcttcat
     1021 cgattattgt atacatcgca ccacgtcccg atacctaatt cctttttggg agtgaagatg
     1081 accctataaa atcgaaacaa gctgaaatct tcccgaaata ggagtcaaaa ataggaatgc
     1141 ataggaacga ataggggtga aacactcccc aagaggagtg aaacactccc aaaaaaggag
     1201 tggagccggc taactctttt ttggctttgg tttttcttcg ggagccgatc cggaaaggac
     1261 gttttggata gagtgcttct tccacggctt gggagcaaag ttcatcaagt gtccggcgat
     1321 gtccttttcg cctctcggcg gggggtgatc cacgagtgcg cgtggcaccc agcttttcag
     1381 tgttatgttc ttctccgata gcggtgctga cttcaccggc gctgactttg atagcgattc
     1441 ttccggtgcc ccctccgccg ttgatggctc gcctgattgc gcctgtttcg atagcagccc
     1501 aatgctttcc aggaaatcta ggacaacgtc atggttcaat cggattccaa agacctggtg
     1561 gtcggcgact tctcgcccgt caaattccct ccattctgcc gaggccggtg ccgtccagcg
     1621 cgcaaagcca ttccagggct cgatggtgca gttgatgtcg cgtcccggat cagagcgcca
     1681 gaagtccccg tcatagggac tgggcggcac cacccctcct ggccacacct ctctcaacca
     1741 gagtttgtag cggatgcggc cgtcgcgatc gcgctcaccc tcttcgagaa atcgctgcac
     1801 caatttgttg gcgaactttt ttgacgatct ggtcagcctg aggaaaagcg catactcgtc
     1861 gcacagcagg cgaaatgtgc tattgtcgtt gtctgttccg tttgtcccgg ccatgggctt
     1921 accacttgat ggctagggat ttgagtcggc gggcgctaac aacgtcccgc cgactcgcct
     1981 gttctacctc cgccgaccgt ggaggtctac gacccttccc ttcgccgact tgccggtcac
     2041 cagctccatg atgtgcgcgc cccagagatc cagcgcacgg cgtctttcct caaggtagat
     2101 ggccttattg taagtgccgg cgacgccgcc cttgtgtcca ccgacatgac cgagaatttc
     2161 ctccaccaca tgcggcggga tgccaaggcg atcgtgcaag gcggtcgaca cgctgcggcg
     2221 gaaatcgtgc agccgccatt ccagcgcctt gccatggctc gccgcaatgc gcgcatccag
     2281 atcgactttg cccctcgacc agttctggta gccgcatccg acgatagtgc caaaaacatg
     2341 ctcccgaggc gtaccatccg cgttggtgcg cttcggaagt gttttcagga tgctgagcgc
     2401 cggctccgac aacgggatca tatgctcgcg tcccttcttg gttcgcgttg acggcagcgt
     2461 gacgagaggc aaggtgggcg agatttcgcc ataggtgaga ccgccgatct cggaacgacg
     2521 cgccccggtg agcatgagga gcctgacgat cgccccaaac tggctgtcat cggctgctag
     2581 ccagatggtt cgtagttctt catcagaaag cactcgctcg cgcgccttct cctccgcctt
     2641 gttggtgaac gcgaccggat tgttgtcgac gtagccctcc ttcgccgccc aggtgaaata
     2701 ggccgacaac gatgctcgca cccggttcct ggcggccggg ccattgtgct tctcgatctc
     2761 ggccagtcga gcggcgacgg tacgcctggc gactgcctct ataggcgagc catgcaacgc
     2821 tttggcgtgg cgctggagat ggcgctcggt ctcgacatag ctgcggggct tgagacgcgt
     2881 gcgctggcgc tccaggaagc gcggcagcaa ggcgccgaag gtctcggcgg ccctgaccct
     2941 ggcgcggtcc ttctcggcgg ccgggtcgcg accgagccga acttgcgcca acaaatcctt
     3001 ggcggtggcg cgcgccttgc cgggatcaag cgcgctcgca agtccgagca cgacgcggcg
     3061 ggtacgcccg gcgatggcgt actgaatcat ccacgaatgc acgccgctgg cgcgcacgcg
     3121 taggccgaaa ccgggcaagt cctcgtcgaa atagaccctg tcggtcgcgc ccggcggcaa
     3181 tttcagagcg cgaatggtgg cggcggtcag cctcatcatc gcgatctcct caagagacat
     3241 ccaagagaca tctgctaacc gatcttaact gatctcaacc ggtcattgga ctgatgatgt
     3301 atcgccgact tgtgcctgga ttacaagggc taaacgcgct taatcgttcc taaacgtttc
     3361 taagcgattt tctgaaacag accgagcgcg gtgcggcacc agatcagcac tatgatgatg
     3421 cgcatgagct ggaacccggc gatcagcgtc gaatcgaggc ccagcacctt gccggtgagc
     3481 accatctcgg cgatgccggc gggcgccatg gccagcacca tg
//
LOCUS       DATLJT010000529         5821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_719752,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000529 DATLJT010000000
VERSION     DATLJT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719752"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VM910_12290"
     CDS             <1..847
                     /locus_tag="VM910_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HVI63346.1"
                     /translation="VVTIAAPSDPSHVTGLFRDRLEDIRKDGEAEVSLAGRAFTIKRE
                     FLDDVAEHNLMADIGKLHRALLVMHSPTDDTVGIENATRIFVTARHPKSFVSLAGADH
                     LLSQKRDSTYVADVIAAWATRYIDAGATEQPVNTGEAPRRVVVRETRDSKFQNAVTVG
                     PHRIVADEPVAAGGEDSGPGPYDLLLAALGACKSMTMRLYADRKSFPLERATVTLAHS
                     KIYAKDCAECETREGMLDQIEVAIGLEGPLDADQRKRILEIADKCPVHRTLTSEIHIV
                     TRAAD"
     gene            931..1878
                     /locus_tag="VM910_12295"
     CDS             931..1878
                     /locus_tag="VM910_12295"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HVI63347.1"
                     /translation="MPAVARIDPKTVFTSEEWSRLTSRSAWRPLWLVVHAWGTIAAAV
                     ALVALWPNPLTWLIAVMIVGTRQLGLAILMHEAAHGGLSTNKTINEFVGQWLCAVPVG
                     ADLASYRTYHLAHHRFTQQPEDPDLSLSAPFPISKESYRRKAFRDLSGQTFVKQRLPL
                     FLSLFKRSAGDEPVTHESFLASGRDKMARFLCVNALLFVLFWLAGAGIWYFGVWLVAM
                     ATRLSLVTRIRNIAEHACTSTHEDPFSHARTTLAGPIERALIAPYWVNYHAEHHLFMY
                     LPCYRLPEAHRLLVEKGLVKRMEVRPGYLEVMRLATSRT"
     gene            complement(1875..2930)
                     /locus_tag="VM910_12300"
     CDS             complement(1875..2930)
                     /locus_tag="VM910_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HVI63348.1"
                     /translation="MDSNHIAVFAGLMIGLVYGSVGLLSGFCMVSSLRGWWAQGDGRL
                     VRTYALAMGIAIAASQLLAERGSIDLTKSIYLQPQFSAPVMFFGGLLFGYGMVLSNGC
                     GSRALVLLGRGNLRSFVVVIVLGIFAEITLKGLIAPARIAAIGVSQVTVKATSLPAVL
                     SSAGMSMQQARGFSASLISSALIIFALAHPPFRRSPGQVVAGVMVGLLIAAGWYATGY
                     LGADEFNPVPVASLTFVAPIADALQYVMLSTGSTLNFGIATVGGVLLGSLVTALATGR
                     FHLEGYQSPQHMLRSAGGAALMGIGGVMALGCSVGQGLTGLSTLAFASFIAVAGILLG
                     AAAGLRGALRVRPLAAT"
     gene            complement(3043..4056)
                     /locus_tag="VM910_12305"
     CDS             complement(3043..4056)
                     /locus_tag="VM910_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HVI63349.1"
                     /translation="MSPVSKSSDFKVEARCVRLAAKADDVASLAPAVERRAISHSAEQ
                     VLIEVKAAAVNPSDVKAATGLMPYAVFPRTPGRDYAGVVIDGPPGSVGREVFGSSGDL
                     GIRRDGTHATHLAVELEALVDKPRSISWEEAAGIGVPFVTAMEGFRRACLPKPGEIVL
                     VMGVNGKVGQAAVQIATWRGARVIGVVRKSEPYEGHANSAVEVIDASSTDVAARVREL
                     TGGHGADIVFNTVGDPYFSAAHRSLALRGRQILIAAIERVVQFNILEFYRGQHTYVGV
                     DTLGLSSIATGEVLRELAPGFAGGYLKPFPINAAAIYPLEEAKRAFVAVAGSSRDRVI
                     LKP"
     gene            complement(4222..5553)
                     /gene="paaK"
                     /locus_tag="VM910_12310"
     CDS             complement(4222..5553)
                     /gene="paaK"
                     /locus_tag="VM910_12310"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HVI63350.1"
                     /translation="MGSTKLKPEAGRYVPELDDAERASRDEMMALQTKRLAWSLKHAY
                     ENVAHYRRAFEAAGVHPSDFRELSDLARFPFTVKTDLRDNYPFKMFAVPREKLVRVHA
                     SSGTTGKPIVVGYTRADIDTWSDVMARSIRAAGGRTGMLLHNAYGYGLFTGGLGAHYG
                     AERLGCTVVPVSGGMTERQVQLINDFRPDIITVTPSYMLAILDEFKRQGLDPRKSSLK
                     FGIFGAEPWTNAMRAEIEQAFDMDATDIYGLSEVIGPGVAQECVETKDGLHIWEDHFY
                     PEVIDPETGKVLPDGEKGELVFTSLTKEAFPIIRYRTRDLTRLLPGTARPGMRRMEKV
                     TGRSDDMIILRGVNVFPTQIEEALLATEWCGGHFVIELTREGRMDEMTVLAEARHSHW
                     DGSGLPDHAEKVSLFIKNTIGITARIIAVAPDTLERSLGKAKRVYDKRPKG"
     gene            complement(5575..>5821)
                     /locus_tag="VM910_12315"
     CDS             complement(5575..>5821)
                     /locus_tag="VM910_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HVI63351.1"
                     /translation="FACNSHNARAVAAQGHITFIKPGKLGDVLVATAREVSRRGRSGI
                     YDVSVTSGHDVIAEFRGHSRTIPGTWLPTNETDKKQK"
BASE COUNT         1105 a         1894 c         1804 g         1018 t
ORIGIN      
        1 ggtggtcacc atcgctgcac cgtcggatcc ctcgcatgtc accggtctgt tcagggaccg
       61 tctcgaggac atccgcaagg acggcgaagc ggaagtctcg ctcgcgggcc gcgccttcac
      121 catcaagcgc gaattcctcg acgatgttgc cgagcacaac ctgatggccg atatcggcaa
      181 actgcacagg gcgctgcttg tcatgcactc gccgaccgac gacacggtcg gcatcgagaa
      241 tgccacccgc atcttcgtga cggccagaca tccgaaaagc tttgtctcgc tcgcgggcgc
      301 cgatcatctc ctaagccaga agcgcgactc gacctacgtc gccgacgtga tagcggcctg
      361 ggccacgcgc tacatcgatg ccggcgcgac cgagcaaccc gtgaataccg gcgaagcgcc
      421 gcgccgggtc gtggtgcgcg agacccgcga cagcaaattc cagaacgcgg tcacggtcgg
      481 accacatcgt attgtggccg acgagccggt cgctgccggc ggcgaggatt ccggacccgg
      541 accctacgac ctgctgctgg ctgcgctcgg cgcttgcaaa tcgatgacca tgcggcttta
      601 cgccgaccgc aaatcgttcc cgctcgagcg ggcgaccgtt acgctcgccc acagcaagat
      661 ctatgcgaag gactgcgcag aatgcgaaac acgggaaggc atgctcgacc agatcgaggt
      721 tgcgatcggc ctcgaaggtc cgctcgatgc cgaccagcgc aaacgcatcc tcgaaatcgc
      781 cgacaaatgc ccggtgcacc gcacgctgac ctccgaaatt cacatcgtga ccagagcggc
      841 ggattaacgt aataatgagg tgcatcgcgt gtccggcgaa ccgagcagcg cttcgctcaa
      901 ggaccgcatc actccgcaga gagcctgccc atgcccgccg tggcacgcat cgatcccaag
      961 accgtcttca catcagagga atggagccgc ctgacctcgc gcagcgcgtg gcgcccgttg
     1021 tggctggtcg tgcacgcctg gggcacgatt gcggcggcgg ttgcgctggt cgcgctttgg
     1081 cccaatccgt tgacctggct gatcgccgtc atgatcgtcg gcacgcgcca gcttggactg
     1141 gccatcctga tgcacgaagc tgcccatggc gggctctcga ccaacaagac aatcaacgag
     1201 tttgtcgggc aatggctgtg cgcggtgccg gtcggcgccg atcttgcgag ctaccgcacc
     1261 tatcatctgg cgcatcacag gttcacccag cagccggaag atcccgatct gtcgctctcg
     1321 gcgcccttcc cgatttcaaa ggagagctat cggcgcaagg catttcgcga tctcagcggg
     1381 caaacattcg tgaagcagcg cctgccgctg ttcctctcgc tgttcaagcg ttcagccggc
     1441 gacgagccgg tcacgcatga gagctttttg gcgagcgggc gggacaagat ggcgcgcttt
     1501 ctttgtgtga acgcgctgct gtttgtcctg ttctggcttg ccggcgcggg catctggtat
     1561 ttcggcgtct ggctggttgc gatggcgacc aggctgtcgc tggtgacgcg catccgcaac
     1621 atcgccgagc acgcctgcac ctcgacccac gaggatccgt tcagccacgc ccgcactacg
     1681 ctggccggtc cgatcgagcg ggctctgatt gcgccctatt gggtgaatta tcacgccgag
     1741 catcacctgt tcatgtatct gccgtgctac cggctgccgg aggcacaccg cttgctggtc
     1801 gagaagggcc tggtcaagcg catggaagtg cggcccggct atctcgaggt gatgcggctt
     1861 gcgacgtcgc gcacttaagt agcagccagc ggccggaccc gcagcgcgcc gcgcaggccg
     1921 gcggcggcac cgagcagtat gccggcgacc gcgatgaagg aggcgaaagc cagcgtcgac
     1981 aatcccgtca gcccctgccc gaccgaacag ccgagagcca tcacgccacc aattcccatc
     2041 agcgcggcgc ctccggccga gcgcagcatg tgctgcggcg attgatagcc ttcgaggtgg
     2101 aagcggcccg tcgcgagcgc cgtcaccagg ctccccagaa ggactccgcc gacggtcgcg
     2161 atcccaaaat tgagtgtcga tcccgtcgac agcatcacgt attgcaaggc gtccgcgatt
     2221 ggcgcgacaa aagtcagcga tgccaccgga acggggttga attcatcggc accgagatag
     2281 cccgtcgcgt accagcctgc cgcaatcaac aaaccaacca tcactccggc cacgacctgc
     2341 cccggcgatc tccggaacgg cgggtgcgcc agcgcgaaaa tgatcagtgc cgaactgatc
     2401 agggaggccg aaaacccgcg cgcctgctgc atgctcatgc cggcgctcga caacaccgcc
     2461 ggcagggagg tggccttcac cgtcacctgc gaaaccccaa tcgccgcgat ccgtgccggc
     2521 gcgatcagtc ctttgagcgt gatctcggcg aaaatgccga gcacgatgac caccacgaaa
     2581 gaacggagat tgccgcggcc gagcagcacc agcgcgcgtg agccgcagcc gttcgacaga
     2641 accatgccgt aaccgaacaa cagcccaccg aagaacatca cgggcgccga aaactgcggc
     2701 tgcaggtaga tcgacttggt caaatcgatc gacccgcgct ctgccagaag ctgcgaggca
     2761 gcgatcgcaa tgcccatcgc cagcgcataa gtgcgcacca ggcgcccgtc gccttgcgcc
     2821 caccatccgc gcaggctgct caccatgcag aagccggaga gcaggccgac cgaaccatag
     2881 acaaggccga tcatcaggcc ggcaaagacg gcgatgtgat tggaatccat cagtgctccc
     2941 ccgtcatggc gggcgaagcg aagcaatccg gttgtctaac cctgatgaca cttgagatta
     3001 cttgtcgctt cgctcctcgc aatgacggtt tcggtggacg ggtcagggct tcaagatcac
     3061 gcggtcgcgt gaggaaccgg cgaccgcgac aaaggcgcgc ttggcttctt ctaggggata
     3121 tatcgcagcc gcattgatcg gaaacggctt cagatacccg ccggcaaatc cgggcgcaag
     3181 ctcgcgcagc acctcgcccg tcgcaatcga cgacaggccg agcgtatcga ccccgacata
     3241 ggtgtgctgg ccgcgataga actccaggat gttgaactgc acgacgcgct cgatggccgc
     3301 gatcagaatc tgccgcccgc gcagggccag cgatctgtgt gcggccgaaa aatagggatc
     3361 gccgaccgtg ttgaagacga tgtcggcacc atggccgccc gtcagttcgc gaacgcgcgc
     3421 ggccacgtcg gtagaggacg cgtcgatcac ctcgacggcg gaattggcgt ggccttcata
     3481 aggctcgctc ttgcgcacca cgccgatcac ccgcgcgccg cgccatgtgg cgatttgcac
     3541 tgcagcctga ccgaccttcc cgttgacgcc cataacgagc acgatctcgc cgggcttcgg
     3601 aaggcacgca cggcgaaacc cctccattgc ggtgacgaag ggaacgccga tgccggcagc
     3661 ctcctcccag gagatgcttc gaggcttgtc gacaagagct tccagctcca cggcaaggtg
     3721 ggtggcatgc gtgccgtcac ggcggatgcc gaggtcgccc gaggagccga acacttcgcg
     3781 gccgaccgaa cccggcgggc cgtcgatcac cacgccggca taatcgcggc cgggcgtgcg
     3841 cgggaacacc gcatagggca tcagcccggt tgcggccttc acgtcggagg gattgacggc
     3901 tgcagccttg acctcgatca gcacctgttc ggcgctatgc gagatcgcgc gccgctcgac
     3961 cgccggcgcg agcgaagcga cgtcgtccgc cttggcggcg agccgcacgc agcgcgcctc
     4021 gaccttgaag tcgcttgatt tggaaaccgg gctcattgaa gggatcgatt agtgcaagag
     4081 ccgagttcaa acaagccttg tcgtgatcga tgtaccctca tcctgaggag cggcgtcttt
     4141 gacgcgtccg gacgggcgag gccgcggtcg gggcctcatg gctcaagatg gtgccttgcg
     4201 cctcctcacc atcggggagc gtcacccttt cgggcgcttg tcgtaaaccc gcttggcctt
     4261 gccgagcgag cgctccagcg tatccggggc gaccgcgatg atgcgagccg tgatgccgat
     4321 cgtgttcttg atgaacagcg agaccttttc ggcatggtcg ggcaagccgc tgccgtccca
     4381 gtgcgagtgg cgggcttccg ccagcaccgt catctcgtcc atccgtccct cccgcgtcaa
     4441 ctcgatgacg aaatggccgc cgcaccactc ggtcgcgagc agcgcttcct cgatctgggt
     4501 cgggaacacg ttgacgccgc gcaggatgat catgtcatcg gagcggccgg tgaccttctc
     4561 catgcgccgc attccgggcc gcgcggtacc cggcagaagc cgcgtgaggt cgcgggtacg
     4621 ataacgaata atggggaacg cttccttggt cagcgaggtg aaaaccaatt cgcctttttc
     4681 gccgtccggc aacaccttgc cggtctcggg atcgatcacc tcgggataga aatgatcttc
     4741 ccagatatgc aggccgtcct tggtttccac gcattcctgc gcgacgccgg gaccgatgac
     4801 ttccgacagg ccgtaaatat cggtcgcatc catatcgaaa gcctgctcga tctcggcgcg
     4861 catcgcattg gtccagggct cggcgccgaa aatgccgaac ttcagcgatg atttgcgagg
     4921 gtcgagccct tgccgtttga actcgtcgag gattgcgagc atgtagctcg gcgtcaccgt
     4981 gatgatgtcc ggcctgaagt cgttgatgag ctgcacctgc cgctcggtca tgccgcccga
     5041 taccggcact acggtgcaac caagccgctc tgcgccgtaa tgcgcgccga gaccgccggt
     5101 gaagaggccg taaccgtagg cattgtgcag aagcatgccg gtgcgcccgc cggctgcgcg
     5161 gatcgagcgg gccatcacgt cagaccaggt atcgatgtcg gcacgcgtat atccgaccac
     5221 gattggtttg ccggtcgtgc cggaagaggc gtgcacccgc accagctttt cgcgcggcac
     5281 cgcgaacatt ttgaagggat aattgtcgcg cagatccgtt ttcacggtga acggaaacct
     5341 tgcgaggtcg gacagctccc tgaaatcgga tgggtgaacg ccggccgctt cgaaggcgcg
     5401 tctgtaatga gctacatttt cgtaggcgtg cttgagcgac caggcgaggc gctttgtctg
     5461 caacgccatc atctcgtcgc gcgaggcccg ttcggcgtcg tccaactcgg gaacgtagcg
     5521 ccctgcttca ggctttagct ttgtcgaacc catcacgcgt ttcctcaaaa tgattcactt
     5581 ttgctttttg tcggtttcat tggtgggcag ccaggtgccc ggtatggtcc gcgaatgccc
     5641 gcgaaattcg gcgatcacgt catgacccga agtgacgctt acgtcgtaga tgccggatcg
     5701 gccgcgccgg gaaacctcgc gcgcggtcgc gaccaggacg tcgccgagct tgcccggctt
     5761 gataaaggtg atgtggcctt gcgctgcgac cgcgcgcgcg ttgtggctgt tgcaggcaaa
     5821 g
//
LOCUS       DATLJT010000530         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3248200,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000530 DATLJT010000000
VERSION     DATLJT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248200"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1219)
                     /locus_tag="VM910_12320"
     CDS             complement(14..1219)
                     /locus_tag="VM910_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI63352.1"
                     /translation="MSSSYRIAIVGGGLAGVAAAHALKAFGMKPEVFEAAPALGEIGA
                     AVNVSPQAFKALQAIGLGDKVAAVATASPGIYTRNMQTGEFLEFNDRHKAAARYGAPY
                     YSFHRADLLDALASGLDHRAIYLGHRLTGLQERSDRIILTFANGAQTEAEIVIGADGV
                     RSVIRRSLYGDDNPTYTGQMVWRALLNADDVPEEVLEPTGHIQWVGPGRHLLSYYIRG
                     KKLVNIVTQEDTDQWVEEGWSIRGDPDEMRLSFPNPEPRLKKLLSLVTECSKWGLFTR
                     PLTQNWGRGRIELIGDAAHAMLPNAGQGACQAFEDAYILARWLDACRDPVEAFTHFRR
                     IRIPRVHGVQRLSLSNARFKHMRDSAAQKESIASGTGSVHGNSDWVWGYDPVGGWDKE
                     PFVPTAYAA"
     gene            1377..2330
                     /locus_tag="VM910_12325"
     CDS             1377..2330
                     /locus_tag="VM910_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63353.1"
                     /translation="MKCWLIALLAVVAIAAPARAQTFPNKTIRIIVPYPVGGSIDLTA
                     RVIAKNLQDSVGHNVIVENKPGANAAIGIEALLRSDPDGHTLVILSDSPVTINVHLSR
                     LNYDPLTDLVPITKVVSSPIILAANAKAGIASIADLVATAKAKPLSYAVAARGSASHL
                     AAELLQRELGITMQAVPYRGGAPAAVAIASGEVPLGLFDTASILPMISSGQVVALGVA
                     ESVRSHSMPNIPTLRESGVPNFSAPSWLALFAPRGTADDRVVRLNAEIAKIAAMPEAQ
                     KVLFAAGLEPAPNSPSEMRSIIAADYKKWGQLIEATGLKAE"
BASE COUNT          430 a          737 c          748 g          444 t
ORIGIN      
        1 agtctttcga gactcatgcc gcataggcgg tgggaacgaa aggctccttg tcccatccgc
       61 cgactgggtc gtaaccccaa acccagtcgg agttgccgtg cacgctgccg gtgccagagg
      121 cgatcgactc cttctgcgcc gcgctgtcgc gcatgtgctt gaagcgagca ttggacaggg
      181 acagccgttg caccccatgc acgcgcggga tgcggatgcg gcggaaatgc gtaaaggcct
      241 cgaccggatc gcggcacgcg tcgagccaac gcgcgaggat gtaggcgtcc tcgaaagcct
      301 ggcaggcgcc ttgcccggca ttaggcagca tcgcatgcgc ggcgtcgccg atgagctcga
      361 tgcgaccgcg gccccaattc tgcgtcagcg gccgcgtaaa gaggccccat tttgagcact
      421 ccgtcacgag gctcaaaagc ttcttgaggc gcggctcggg attcggaaag ctcaggcgca
      481 tctcgtcggg atcgccgcgg atggaccagc cttcctccac ccattggtcg gtgtcttctt
      541 gggtgacgat gttcacgagc ttcttgcccc ggatgtaata ggaaaggaga tggcgacccg
      601 gcccgaccca ctggatatgt cccgtgggct cgagcacctc ctcgggaacg tcgtcggcgt
      661 tgaggagcgc acgccacacc atctggccgg tataggtcgg gttgtcatcg ccatagagcg
      721 agcggcggat gaccgagcgc acgccatcgg cgccgatgac gatttccgcc tcggtttgag
      781 cgccattagc gaaggtgagg atgatgcggt cgcttcgctc ctgtagtccc gtcaggcggt
      841 gccccaggta aatggcgcga tgatcgagtc cgctggcgag cgcgtcgagc agatcggcgc
      901 ggtgaaagct gtaataggga gcgccgtagc gcgccgcggc cttgtgccga tcgttgaatt
      961 cgaggaactc gccggtttgc atgttgcgcg tgtagatgcc gggcgatgcg gtcgcgaccg
     1021 cggcgacctt gtcgccgaga ccgattgctt gcagcgcttt gaaggcttgc ggactgacgt
     1081 tgaccgcggc gccgatctcg cccaaggccg gtgcggcctc gaagacctcc ggcttcatgc
     1141 caaaagcctt tagcgcgtgc gcggcggcga ccccggcgag cccgccgccg acaatcgcga
     1201 tacgatagct tgacgacatt cgtccctccg catcctcgtc gccaccgttc cagcacgtct
     1261 gctggagcat agcgcgtagc tcggagcgaa gctaatccga gctttgtggg cttgctcctt
     1321 acgccgcgtt ccgtccatca tcggagcgga cgacccgaag tcgaagacga atattcatga
     1381 agtgttggct gatcgctttg ctcgctgtcg tcgccatcgc cgcaccggcg cgtgcgcaga
     1441 cattccccaa caagacaatc cgaattatcg tgccttatcc cgtcggcggc agcatcgatc
     1501 tgactgcgcg cgttatcgcg aagaacctgc aggatagcgt tggacacaac gtcatcgtgg
     1561 aaaacaagcc gggtgcgaat gcggccatcg gcatcgaagc cttactgcga agcgatccgg
     1621 acggccatac gcttgttatc ctctcggata gcccagtcac gatcaacgtg catctgtcgc
     1681 ggctcaatta cgatcctctt accgacttgg taccgattac gaaggtagtg agcagcccga
     1741 tcatcttggc cgccaacgcc aaggcgggga tcgcatcgat tgccgatctt gtcgcgaccg
     1801 cgaaagccaa gcccttgagc tacgccgttg cggctcgcgg ctcggcgtcc cacctggcgg
     1861 ctgagttgct ccagcgcgaa ctcgggatca cgatgcaggc cgtgccctat cgcggaggcg
     1921 caccggcggc ggttgcgatc gcctccggcg aggtcccctt gggattgttc gacacggctt
     1981 cgattttgcc gatgatcagt agcggccagg ttgtcgcctt gggcgtggcc gagtccgtgc
     2041 gttcgcactc catgccgaac attccgacgc tgcgtgagtc gggcgttcct aacttcagcg
     2101 ctccctcgtg gctggcgcta ttcgccccgc gcggcacggc ggacgaccgt gtcgttaggc
     2161 tcaacgccga gatcgccaag atcgcggcaa tgccggaagc gcaaaaggtc ctattcgccg
     2221 cgggcctcga gcctgccccc aacagtccga gtgagatgcg cagcatcatc gcagcggact
     2281 acaagaaatg gggtcagttg atagaggcga cgggcctcaa ggccgagtaa tgcgaatcta
     2341 tcgaagccag gagtcgaca
//
LOCUS       DATLJT010000531        10889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2098787,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000531 DATLJT010000000
VERSION     DATLJT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098787"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VM910_12330"
     CDS             <1..226
                     /locus_tag="VM910_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HVI63354.1"
                     /translation="DLFVTITIEEKQALIGTSRLMLMVGAKDGQLKQWTVTDPQGYDT
                     TVAVYNLDSSKKIDPGMFKIDFTNYQTPPG"
     gene            401..1216
                     /locus_tag="VM910_12335"
     CDS             401..1216
                     /locus_tag="VM910_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVI63355.1"
                     /translation="MRLSLTTWNINSVRLRINLVAKFIRAFRPDVLCLQETKCIDDAF
                     PTKRFKRLGYEHIALNGQKGYHGVAIISKLPFETTDIRTFCDKIDSRHISVAFGAKAE
                     LAKPLVLHNFYVPAGGDIPDPGLNPKFDHKLKFLDELKACEPLHPRGDDRHILVGDLN
                     VAPHENDVWSHRQLLKVVSHTPIECEKLLAAQSEGDWLDVARERIPLSEKVYTWWSYR
                     AADWTVGDRGRRLDHIWVSRALKDQVSDFAILRDARGWDRPSDHVPVTVTLEV"
     gene            complement(1213..1656)
                     /locus_tag="VM910_12340"
     CDS             complement(1213..1656)
                     /locus_tag="VM910_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI63356.1"
                     /translation="MSIDDDVALLERVPTLRLLGSAALRMLAIGSEQRDVARGNVLFN
                     QGDEADCGFVVQRGALRIDDGTGAEAVARPGMLIGELALVVAMKRPSSAVALEHSTVI
                     RIARSLFQRVLESDPDAARRLRDEFANRTSQIASDLLTAGAKLSN"
     gene            complement(1713..2399)
                     /locus_tag="VM910_12345"
     CDS             complement(1713..2399)
                     /locus_tag="VM910_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI63357.1"
                     /translation="MANARKILIVDDDTDLRDTLVEQLSLHDEFEASAVDTGAKGASA
                     AKANSPDLVLMDVGLPDTDGREVVRSLRKGGFKAPIIMLTGHDTDSDTILGLESGAND
                     YVAKPFRFAVLLARIRAQLRQHEASEDAVFSVGPYSFRPGSKMLTGANARKVRLTEKE
                     TAILRFLYRAGQLPVSRETLLQEVWGYNSGVTTHTLETHIYRLRQKIEKDAANPEILV
                     TEAGGYKLVP"
     gene            2614..3180
                     /locus_tag="VM910_12350"
                     /pseudo
     CDS             2614..3180
                     /locus_tag="VM910_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
     gene            3203..3988
                     /locus_tag="VM910_12355"
     CDS             3203..3988
                     /locus_tag="VM910_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI63358.1"
                     /translation="MLEKEQIASAARTLQSHWRAGTKLAALDPATRPRNRVEGYAIQA
                     ELERSSSRPLFGWKIAATSEAGQKHINVAGPMAGRILAENVIADGGTASMQGNEMRVA
                     EPEFAFRMARDLPPRASPYSVAEALDAVATLHPAIEIPDSRFADFVSAGEAQLIADDA
                     CAHLFVLGAPTSADWRARDLVAEKPVISLRGKTHIGHGKNVLGDPRVALAWLVNELRG
                     LGLTLRAGEVVTTGTCHPPLEIRSGDLVAADFGVLGKVSVGLR"
     gene            complement(4052..4744)
                     /locus_tag="VM910_12360"
     CDS             complement(4052..4744)
                     /locus_tag="VM910_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HVI63359.1"
                     /translation="MTPQNPASVTPHSPNGLASVEQEIARACQEAKRERSSVTLIAVT
                     KTFGADAISPVIESGQRVFGENRVQEAKAKWPALMSAYPGLVLHLIGPLQSNKAKDAV
                     ALFDAIHSVDRPSVCQALAKEIESRKKTPQLFVQLNTGEEPQKAGVKPSEADAFIASC
                     RDNYGLTISGLMCIPPVNEAPAPHFALTAKIAARNGLKNLSMGMSADFATAIQFGATH
                     IRVGSAIFGHRG"
     gene            4853..6550
                     /locus_tag="VM910_12365"
     CDS             4853..6550
                     /locus_tag="VM910_12365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVI63360.1"
                     /translation="MFHFSFNRNKVKLKQLIGIRARLALLAVILVAPLMLERARSLED
                     ARARQIELASAEFASLARHSADAQREVISSVETVLKSAAYIRASAGGISKGCDILRAS
                     LPGNLPWIRNILIAGGDGRIQCSTNDMFVGLDLSDRAYYRKAQSTHEFVFSDFLISRP
                     TQDATVVAAYPVSAINRDVDAVVLASINLDWMSKVMSNFNGRPGILAALIDSTGVVLA
                     APEDQADLIGKPLDDVPPLSAIAEEALGSDRDDGSLSFVAADGSRRALSFARVAGTGA
                     RLIASIDEDKVTAAINNDIRTAYLQLAFVILFVLVGALIAAEKLIIQPIRVMAAMAKR
                     FGQGDFSARAVRSRLPSEFVPLARAFNAMAAQLSQRERELVATNDRLTVIASIDMLSG
                     LANRRGFQSRLDFEWMKAQQHDCDLSLLMIDVDHFKFYNDTYGHPEGDACLTRLGETL
                     SGIAADTVGFAGRYGGEEFCLLLPNTGAGRAREIGEIVRSAVEKLEQPHRTSSYQIVT
                     VSVGVASSRPNAAQSPADLIEAADAARYAAKHRGRNTVVEHDFVRITDNAGGIAMAC"
     gene            complement(6917..8029)
                     /locus_tag="VM910_12370"
     CDS             complement(6917..8029)
                     /locus_tag="VM910_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVI63361.1"
                     /translation="MDSSKPGGFRAIPSATGGIARLACARLLEQGKDVAAVLSKTGLT
                     RREAEDPAIRLEVRTQIKFLELAAEALGDDLLGFHLARGFDLREIGLIYYVMASSERL
                     DDALGDAARYSGINNEGVRLRFAENDAAMIALDYVNVDRSSDRHQAEFWLVALVRICR
                     HVTDGRFAPLRLRMRHFRDGTPAEFKSFFGCDVEFAAEADEITLPTNVASLPVVSRDS
                     YLHNLLHRYAEEALAKRPPQRAGLRADIEKILTLLLPHGRAEASEAARRLGMSARTLS
                     RKLRDQGLTYAGVLDDFRASLAKHYLSEGDLSVSEIAWLLGYREFSSLTHAFKRWTGM
                     TPRQFRLSGDAHQNGAGSPSAKTAAGKTQEERGGGK"
     gene            complement(8345..9121)
                     /locus_tag="VM910_12375"
     CDS             complement(8345..9121)
                     /locus_tag="VM910_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HVI63362.1"
                     /translation="MLKWCSYCQQFMHEIPPYGDFSITHGLCLNCEAEHSDLFATDEL
                     RHAIFLRSIFLALFEAGLKSDFGRAKRIVKEAIAANCRPVDILIGMVAPMLYEIGDGW
                     ERGALTVEDEHRFTAFCEQVVNLIDRRMQSSRSRLPSTDVPALFLMNAPGNTHTLAVR
                     ILALWLESRGIGVRRLVDDRNLEQLTQRLVTSAAKVLLISMALPEQRDGVVRISDLLD
                     RFPAGVRPRLIVGGYAVKVGLARPIPGAELMADISSLRFN"
     gene            complement(9810..10796)
                     /locus_tag="VM910_12380"
     CDS             complement(9810..10796)
                     /locus_tag="VM910_12380"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVI63363.1"
                     /translation="MTAQRVPPTPNNFNVWFRYVLGAPPELKRTIDILLGNKRPFDAT
                     TNRDLFATYVGSKAMDDAVAHNVSQQLHSVMQSAREFLATAISDNRTQIRAISEVAGR
                     SETGTDPKLLVETLMNELAKAATRAARLEARFAEKSRELDLIRESLTKSEERARTDAL
                     TGLPNRRALEEFFRTAQTSVMEQGQPLSAMLIDIDHFKRFNDSFGHGVGDQVLRLVAK
                     ALRERIREVDLPARYGGEELIAVLPGADLTAATATAERIRRSISECKITRRSTGEVLP
                     GITVSIGVGQFQFGESMDDLIEHCNRALYQAKKSGRNLVVAENELAHARYVG"
BASE COUNT         2122 a         3359 c         3310 g         2098 t
ORIGIN      
        1 cgatctcttc gtcaccatca ccatcgagga gaaacaggcg ctgatcggca ccagccgtct
       61 gatgctgatg gtcggcgcca aggacggcca gctcaagcag tggaccgtga ccgatcccca
      121 gggctatgac accacggttg cagtctataa cctggattcc tccaagaaga tcgatccggg
      181 catgttcaag atcgacttca ccaactacca gacaccgccc ggctagcatt ccctccgtca
      241 ttgcgagcga ccgtgacctt tctgcgctcg ctgcgcgagc ttcgcagcgc ggaagggcgc
      301 gaaacgcatc cgcgcgcagg cggaagcgat cccggatttt cctgtttgcc ggattgcttc
      361 gtcgcttgcg cttctcgcga tcagcatcat aactgcctcc atgcgtctct cgcttacaac
      421 ctggaacatc aactcggtgc ggctgcgcat caacctggtc gccaaattca tcagggcgtt
      481 tcggccggac gtactttgcc tgcaggagac caagtgcatc gacgacgcct ttccgacgaa
      541 gcgtttcaag cggctgggtt acgagcacat cgcgctgaac gggcagaagg gctatcacgg
      601 cgtcgccatc atctcgaagc tgccgttcga gaccaccgac atccgcacct tctgcgacaa
      661 gatcgattcg cgccacatct cggtcgcctt cggcgcgaag gccgagctcg caaagccgct
      721 ggtgctgcac aatttctacg ttcccgccgg cggcgacatt cccgaccccg ggctcaatcc
      781 gaaattcgat cacaagctca aattcctcga cgaactgaaa gcctgtgaac cgcttcatcc
      841 acgcggcgac gaccgccata tcctggtcgg cgatctcaac gtcgcgccgc acgagaatga
      901 cgtgtggtcg cacaggcagc ttctgaaggt cgtctcgcac acgccgatcg agtgcgaaaa
      961 gctcctggcc gcgcagtcgg aaggcgactg gctcgacgtg gcgcgcgagc ggatcccgct
     1021 ttcggagaag gtctatacct ggtggagcta tcgcgcagcc gactggacgg tgggcgatcg
     1081 cggccgacgt ctcgaccaca tctgggtgtc acgcgcgctg aaggatcagg tcagcgattt
     1141 cgcgatcctg cgggatgcgc gcggctggga tcggccctcc gatcacgtgc cggtcacggt
     1201 gacgctcgag gtttaattgc tgagcttggc gcccgccgtc aggaggtcgc tcgcaatctg
     1261 gctggtgcgg ttggcgaatt catcgcgcag gcgccgcgcc gcgtccgggt cgctctccag
     1321 cacgcgctga aacaggctgc gcgcgatgcg gatgacggtc gagtgctcca gcgcgaccgc
     1381 gctcgacggc cgcttcatcg caaccaccag cgcgagttcg ccgatcagca tgcctgggcg
     1441 cgcgacggcc tcggcgccgg taccgtcgtc gatccgtaat gcgccacgct ggaccacgaa
     1501 gccgcaatcg gcctcgtcgc cctggttgaa gagaacattg ccgcgcgcga catcgcgctg
     1561 ctcggaaccg atcgcaagca tgcgcagcgc tgcggagccc aacaaccgca gggtcgggac
     1621 ccgctccagc agtgcgacat catcatcgat cgacataaag taccggtgat cgggacaggc
     1681 agattaaatt cgatttcgcc gtcccgaacg tatcacggca ccagcttgta gccaccggct
     1741 tccgtcacca ggatctcggg attggcggcg tctttctcga tcttctggcg cagccggtaa
     1801 atgtgggttt ccagcgtatg cgtggtgacg ccggaattat aaccccacac ttcctgcagc
     1861 agggtttcgc gcgacaccgg aagctggccc gcccgataga ggaagcgcag gatcgcggtt
     1921 tccttctcgg tcaggcgcac ctttcgcgca ttggcgccgg tgagcatttt ggagccgggc
     1981 cggaagctgt agggaccgac cgagaacact gcgtcctcgc tcgcttcgtg ctggcgaagc
     2041 tgcgcgcgga tccgcgccag caacaccgca aagcggaacg gctttgcgac ataatcgttg
     2101 gcgccggatt ccagcccgag gatcgtatcc gaatcggtat cgtgcccggt gagcatgata
     2161 atcggcgcct tgaagccacc cttacgcaag gatcgcacga cctcacggcc atcggtatcg
     2221 ggcagcccca catccatcag cacgagatcg ggcgaattgg ccttggcagc gctggcgccc
     2281 ttggcgccgg tatcgacggc agaggcttca aactcgtcgt gcagcgacaa ttgctccacc
     2341 aacgtatcgc gcagatcggt gtcgtcatct acgatcagga tcttgcgggc attggccatg
     2401 gtacattcct attgacggcg gcagggtgcg agcagaagcg ccttcagccg aagtggtcag
     2461 gtgggtcgtc acgtctcgtt tcgtcttgag gtagtgaatc gttaccgatt cacaacccaa
     2521 gcgcagtcta ctccaaaaat cgcgggcaat ttgatgaatg tcctgtaatc aaacacgtct
     2581 ccggcaggcg cattcatcgg attcagggtt cccatgaaaa agaaaataac ttcaaaggtt
     2641 tgcgcaggaa ccccgcgtca ttgcccgctc tccctgatcc agatcaggcc ggccgctgga
     2701 aattcccgaa ggggctggct caaggcaggg aaccgaagca tcccggtggc gcttggccga
     2761 ggcggcatcc gcgccaacaa gcgcgaaggc gatggcggca cgccaaaggg catcttccat
     2821 ccgcgccagg tgtggtggcg cgccgaccgg cacgcgcggc cgcgcacttt tctcccgacg
     2881 cgcgccatcc gtgtcgagga tgcctggtgc gaggacccca acgaccgtca ctacaacaaa
     2941 ccgatccgcc tgacgcgcga tcagagcggc gatcggctga ggcgcgacga tcacctctat
     3001 gatttcattg tcgagatcga tcacaacacc aacccgcgtg ttgccggccg cggtagcgcg
     3061 gtgttcctgc accttgcgcg cgggaatttt ggcccgaccg ccggctgtgt ctcgatgacg
     3121 aaatcggcga tgctgcgcct gttgcaacaa ttgtcgcgca acaccaaaat cgtgatcggg
     3181 cgaggggagg gaggtcaacg ccatgctcga gaaggagcag atcgcttccg ccgcgcgcac
     3241 actgcaatcg cattggcgcg ccggcaccaa gcttgccgcg ctcgatccgg cgacgcggcc
     3301 gcgcaaccgc gtggaaggtt acgcgatcca ggccgagctt gaacgcagct cgagccggcc
     3361 gttgttcggc tggaagatcg ccgcgaccag cgaggccggg cagaaacaca tcaatgttgc
     3421 aggcccgatg gccgggcgca tcctggcgga gaatgtcatt gccgatggcg ggaccgcgtc
     3481 gatgcaaggc aatgagatgc gcgtggccga accggaattt gctttccgca tggcgcggga
     3541 tcttccgcct cgcgcgtcgc cttattcggt tgccgaggcg ctcgatgccg tcgcaacgct
     3601 gcacccggcg atcgaaattc cggattcgcg ctttgcggat ttcgtcagcg ccggcgaggc
     3661 gcaactgatt gccgacgatg cctgcgcgca cctgttcgtg ttaggtgccc cgacatcggc
     3721 cgattggcgc gcgcgcgacc tggtggcgga aaaacccgtt atctccttgc gcggcaagac
     3781 ccatatcggg cacggcaaaa acgtgctcgg cgatccccgc gttgcgctgg cctggcttgt
     3841 gaacgaattg cgcgggctgg ggctcacgct gagggcaggg gaggtggtca cgacggggac
     3901 ctgccatccg ccgcttgaaa ttcgatccgg cgacctcgtt gctgcggact ttggtgtgct
     3961 cggaaaagtg tccgtggggt taagatagat tccgcacaca tcgctgtcgt cctggcgaac
     4021 gtcgggaccc atgaccgcgg gcttgagttt tctatccccg atgtccgaaa atcgcggagc
     4081 ccacacgaat gtgggtcgcg ccgaattgaa tggcggtcgc gaagtcggcg ctcatgccca
     4141 tcgagagatt tttcagaccg ttgcgcgcgg cgatcttggc ggtcagcgcg aaatgcggcg
     4201 ccggcgcttc attcacgggc ggaatgcaca tcaagcccga aatggtcagg ccgtaattgt
     4261 cgcggcagct cgcaatgaaa gcatccgcct ccgaaggctt gacgccggcc ttctgcggtt
     4321 cctctccggt attgagctgg acgaacaatt gcggagtttt tttccgggat tcgatttctt
     4381 tggctaacgc ttggcaaacg cttggccggt ccaccgaatg gatggcgtcg aacagcgcga
     4441 ccgcgtcttt cgccttgttg gattgcaggg gaccgatcaa atgcagcacg agcccggggt
     4501 aagcagacat taacgctggc cacttcgctt ttgcttcctg cacgcgattt tcgccgaata
     4561 cgcgttgtcc gctttcgatc accggcgaaa tcgcatctgc tccgaaagtt ttggtgacag
     4621 cgatcagcgt caccgagctg cgctctcttt tggcttcctg acaggcacgc gcgatctcct
     4681 gctcgacgga ggcgagcccg tttggtgaat gcggtgttac ggaggcgggg ttttgcggcg
     4741 tcatctgaga tgcggactat aattttgcgg aggtaggtcg agttttatcg agttcaagaa
     4801 aggctttttg aaccattccc actacgctgc ctcgcggggt aggggaacca caatgtttca
     4861 cttcagtttc aaccgcaaca aggtcaaact caagcagctt atcggaatcc gcgcacggct
     4921 ggcgttgctt gcggtgatcc tggtggcacc cttgatgctc gagcgcgccc gctcgctgga
     4981 agacgcccgt gccaggcaga tcgagctcgc ttccgccgaa ttcgcgagcc tggcaagaca
     5041 tagcgccgac gcccaacgcg aagtgatctc ctcggtcgag acggtcttga agtcggcggc
     5101 ctatatccgc gcttccgccg ggggcatcag caagggctgc gacatcctgc gcgccagcct
     5161 gccgggcaac ctgccctgga tccgcaatat cctgatcgcc ggcggcgacg gacgaatcca
     5221 gtgctcgacc aacgacatgt ttgtcggact cgatctctcg gatcgagcct attaccggaa
     5281 ggcgcagtcg acgcacgagt tcgtgttcag cgattttctg atctcgcggc cgacgcagga
     5341 cgcgaccgtg gtggcggcct atccggtttc cgcgatcaac cgcgacgtcg atgccgtcgt
     5401 cctcgccagc atcaatctcg actggatgtc gaaagtgatg agcaatttca acggccgtcc
     5461 aggcattttg gccgcactga tcgattcgac cggggtcgtg ctggcagcgc cggaagacca
     5521 ggccgacttg atcggcaagc cgctggacga tgtgccgcca ttgtcggcga tcgccgagga
     5581 agcgctcggt tcggacaggg acgatggatc gctgtccttt gtcgctgccg acggctcgcg
     5641 ccgcgcactc agctttgcgc gcgtcgcagg cacgggcgcg cggctgatcg cgagcatcga
     5701 cgaggacaag gtgacggcgg cgatcaacaa cgacattcgc accgcgtatc tgcagcttgc
     5761 tttcgtcatc ctgtttgtgc tggtcggcgc gctgatcgcc gcggaaaagc tcatcatcca
     5821 gccgatccgg gtgatggcag cgatggcaaa gcgctttggc cagggtgact tctcggcgcg
     5881 cgcggtgcgc agccgcctgc cttcggaatt cgtgccgctg gcgcgcgcct tcaatgcgat
     5941 ggcggcgcag ctcagccagc gcgagcgcga actggtcgcg accaacgacc gcctgaccgt
     6001 cattgcatcg atcgacatgc tgtcgggcct cgccaaccgg cgcggattcc agagccggct
     6061 cgacttcgaa tggatgaagg cgcagcaaca tgactgcgac ctgtcgctcc tgatgatcga
     6121 tgtcgatcat ttcaagtttt acaacgacac ctatggccat cccgaaggtg atgcctgcct
     6181 gaccaggctc ggcgagaccc tgtccggcat cgccgccgac accgttggat ttgccgggcg
     6241 ctatggcggc gaggagttct gcctgctgtt gcccaacacg ggagccggcc gggcccgcga
     6301 gattggcgag atcgtgcggt cggcagtcga gaagctggag cagccgcacc gcacctccag
     6361 ctaccagatc gtcaccgtga gcgtaggcgt cgcttcgagc cgcccgaacg cagcgcagtc
     6421 gccggccgac ctgatcgaag ctgcggacgc tgcccgttat gcggccaagc accgcggccg
     6481 caacaccgtg gtggaacacg attttgtgcg gatcacggat aacgccggcg gcattgcgat
     6541 ggcgtgctga ggcggaccgc cgcgactgat cgctgatgcg ttcgggctac gggccgacag
     6601 cggcattgat atgatcgaac tgcaaatcca atatcgcgcg ctcacgatag cacaagccgc
     6661 agattcgcat cagggcgcaa ccagtatcca gatcattgcg gtggcccatc cgagcaacca
     6721 gacgatcgat agccaaaaga ggaaatcgtt tcgcctcatg aaaggcgacg ccggaagctt
     6781 tcttttggat tgctcatctc tggacattcg cggccagcct gtagcaggcc gcagcgacca
     6841 aggtcttgtt aggccacgcc aacaatttgg gatttcacgc cgaggcgctc agaagaaagt
     6901 cttaggctgg gcgccttcac ttgccgccgc cgcgttcttc ttgggtcttc ccggcggccg
     6961 tttttgccga cgggctaccc gcaccgttct ggtgggcatc accggatagt cgaaattgcc
     7021 ttggcgtcat cccggtccat cgtttgaaag catgtgtgag agagctgaac tcccgatagc
     7081 cgagtaacca cgcgatttcg gaaaccgaca aatccccttc gctcaagtag tgtttggcga
     7141 gcgaggccct gaagtcatcc agtacgccgg cataggtcag accttggtcg cgaagttttc
     7201 gcgacagcgt tcgcgcgctc atgccaagcc gtcgcgcagc ttccgaagcc tcggctcgac
     7261 cgtgtggcag cagcagcgtc aatattttct cgatgtccgc gcgaaggcct gcccgctgcg
     7321 gcggccgctt ggcaagcgcc tcttccgcat agcgatgcag cagattatgc agatagctat
     7381 cgcggctgac gaccggtagc gaggcgacat tcgtcggaag cgttatctcg tcggcctccg
     7441 ctgcaaattc aacatcgcaa ccgaagaagc ttttgaactc ggcaggcgtg ccgtctcgga
     7501 aatgacgcat cctcaaccgc aagggcgcga agcggccgtc ggtcacgtgc cggcagatgc
     7561 gaaccagcgc gaccagccaa aactctgcct gatgccgatc tgaactgcgg tccacattca
     7621 catagtcgag cgcaatcatc gccgcatcat tctcggcgaa tcgcaagcga accccttcat
     7681 tgttgatccc gctatagcgc gcggcatcgc caagcgcatc gtccagccgc tccgacgacg
     7741 ccatcacata ataaatcaaa ccgatctcgc gcaaatcgaa gccgcgcgcg agatgaaatc
     7801 cgaggagatc gtcgccaagc gcctcggcgg ccaactcgag aaacttgatc tgggtgcgca
     7861 cctcgagccg tatcgccgga tcttcggcct cccggcgcgt cagccccgtc ttggacaaaa
     7921 cggctgcgac gtccttgccc tgctcgagca gccgggcgca agcaaggcgt gcgatgccgc
     7981 cggtggcgct cggtatcgca cgaaaccccc caggtttgct ggagtccatt gatccggacc
     8041 tcttcgatga tcgactgccc tggagttgcg ggctcaaagc acgaaagatt ggcgcatctt
     8101 cataggtcgc tgcttcccgc ctacgctaaa acagcccgcc cttgcggggc ggatgttcaa
     8161 tgttttgttg gccagccgcg tccattttga tctggatcaa ggcgaaaaac agcggacggc
     8221 cgggagacca tgttaccgac gcccgcaatt gagcgctttg gaccagacta tcccgaccac
     8281 agggagaacc tgctggcaac ccgtctcggt gatcggcgac gtccggagag accgacaagg
     8341 caattcagtt gaagcgcaag gaactgatgt cagccatcag ttcggccccg gggatcggcc
     8401 gagccaaccc gaccttcact gcatacccgc ccacgataag tctgggacgc acaccagccg
     8461 gaaagcgatc caggagatcg gatattcgta cgacgccgtc acgctgctcg ggaagcgcca
     8521 tcgagatcaa aagaactttt gcagcagagg taacgagccg ttgcgtaagc tgctcgagat
     8581 ttcggtcatc gaccaatctc cgcacgccta ttccgcgact ctccaaccag agggccaaaa
     8641 tgcgaacggc caaagtgtgc gtattgccgg gagcgttcat cagaaacaat gcggggacgt
     8701 cggttgaggg caagcgcgac ctgctcgatt gcattcttct gtcgatcaag ttgacaacct
     8761 gttcgcaaaa cgccgtaaac ctgtgctcgt cctcgaccgt gagggcgcca cgctcccagc
     8821 cgtcgccaat ctcgtagagc atgggagcga ccattccaat caggatgtcg accggtctgc
     8881 agttcgcggc aatggcttcc ttgacgatgc gtttggccct gccgaaatcg ctcttgagac
     8941 cggcctcgaa cagagcgaga aagatgctgc gcaggaagat ggcgtgccgg agttcgtcgg
     9001 ttgcaaaaag gtccgagtgt tccgcttcgc aattcaggca caatccgtgc gtgatactga
     9061 aatcaccata gggtggaatt tcgtgcatga actgctgaca ataggaacac catttcagca
     9121 tggcgcggtt tcgggcgttg cgaaatttgg ccgaactatc ctatcgaaac ccacaaacct
     9181 tccaagctgt tagatcacga aagcgcttcg aaaaaagcgc ggctgcagtc gtggagggtg
     9241 catccgccag ggatcaatcc ggcacctcgg cgacatgtcg atccggcgcc cggaagcaag
     9301 cgccatgttt gggccacgaa ccttacattg cccgtcgtgc caatctgccg cagagatgcg
     9361 gcattgctca aaaccccaaa tcacaagcat catccgcgcc gtcccgcctc tgctagaggg
     9421 gcgcttcgcg atcgtcacga gtgttgggag cgggatacga tggacgcggt cggtgtcgcc
     9481 ggacgtacga tatcgacgtg tcggcgaagc cgtgtggtcc tgatgcccca aggttggcgt
     9541 caagcttgca atcgatgatg tgcaggcgac ggagacaaca aagcctggtc tccggggaga
     9601 gcccggaata agccgtcaaa ccatcgtgca gggaatgccg gctgatttcg gtgaacctgt
     9661 agtgactaac tcgtgtgctt tttcattcgc acgcgaggct gcgggtgcat cgagcacccg
     9721 gcattccctg cgccctctct tcaaatggaa gggcccgtga ttgcagagac tcgggcgaaa
     9781 tcgagccgcg ggaacgagat cgcatgttct tatccaacgt atcgcgcgtg agcgagctcg
     9841 ttctcggcga cgaccaggtt gcggccggat ttcttcgcct gatagagcgc gcgattgcag
     9901 tgttcgatca ggtcatccat ggattcgccg aactggaact gacctacgcc aatcgacacg
     9961 gtaatgcccg gcagcacctc gccggtcgag cgccgtgtga tcttgcactc ggagatggag
    10021 cggcgaatgc gttccgccgt ggcggtcgct gccgtgaggt ccgcgccggg aagaacggcg
    10081 atcagctctt cgccgccata gcgcgccgga agatcgacct ccctgatgcg ttcgcgcaac
    10141 gctttcgcca ccagccgcag cacttgatcg ccaacgccat gaccgaaact gtcgttgaac
    10201 cgcttgaagt gatcgatgtc gatcaacatg gcgctgagcg gttgaccttg ctccatgacg
    10261 ctggtctggg cggtacggaa gaactcctcg agcgcacggc ggttggggag cccggtcagg
    10321 gcgtcggtcc tggcccgttc ttccgacttc gtgagcgact cccggatcag atcgagctcg
    10381 cgcgacttct cggcgaagcg cgcctcgagc ctggccgcgc gcgtggcggc tttggcgagt
    10441 tcgttcatca gtgtctcgac cagaagcttg ggatcggtgc ccgtttcgct gcggccggcg
    10501 acctcactga tggccctgat ctgcgtgcgg ttgtcggaaa tggcggttgc cagaaattcg
    10561 cgggcggatt gcatcaccga atgcaactgc tgcgacacgt tgtgggcgac cgcatcgtcc
    10621 atggccttgg acccgacata ggttgcaaac aggtcgcgat tggtcgtggc atcgaagggc
    10681 cgcttgttac caagcaggat gtcgatcgtg cgctttagct cggggggtgc gccaagcaca
    10741 tatctaaacc agacgttgaa attgttcggt gtcgggggta cccgctgcgc cgtaatggag
    10801 cgcatcgcct tatcggcgac gtcagtcgcg aactcgaaat ccaggtcttc gaatgcgtcg
    10861 gccaaaatct tgcgcgcccg cttccatcc
//
LOCUS       DATLJT010000532         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2788980,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000532 DATLJT010000000
VERSION     DATLJT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788980"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VM910_12385"
     CDS             complement(<1..626)
                     /locus_tag="VM910_12385"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HVI63364.1"
                     /translation="MTAFDLFTIGHSNISAERFIALLRGTGIDAIADVRSIPVSRFCP
                     WFSAKNLAPLLGKANIDYLSFGDQLGGRPRDPSLYCDGVVDYEAMAQRPSFQAGLDHL
                     LANAGRRRLCLMCSERDPLDCHRCLLVARALAARGVSFGHILHDGAIESHSAIERRLL
                     QGAGEDSDLLVTGQDERLAAAYRRRARTAAYSIKGAKKALNRTISKRAT"
     gene            782..1204
                     /locus_tag="VM910_12390"
     CDS             782..1204
                     /locus_tag="VM910_12390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI63365.1"
                     /translation="MRPLCCVLAGLVFAVALTNSASAAIVVDIDRSVQRMAVTVDGVP
                     RYNWRVSTARRGYITPPGTYHPEMLARHWFSRKYYNSPMPHSIFFYGDFAIHGSYEIS
                     HLGRPASHGCVRLDPGNAAVLFGLVQREGMSTTTIVIH"
     gene            complement(1329..1463)
                     /locus_tag="VM910_12395"
     CDS             complement(1329..1463)
                     /locus_tag="VM910_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63366.1"
                     /translation="MAGELFSMKKFALPLAAVLALLSIAGCAQYYGKGKAPPPVVTKG
                     "
     gene            1841..>2281
                     /locus_tag="VM910_12400"
     CDS             1841..>2281
                     /locus_tag="VM910_12400"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI63367.1"
                     /translation="MAITVCPVTLGFVAEIGDIDLSTPLAPADLLAVKEAFTKYAVLI
                     FPDQHLSQDAHLNFARNFGPLETTIALFRKDAKLRVRKEFADVSNLDADNKVWGENSR
                     RRMFELGNRLWHTDSSFKHLPARASLLYARSIPPLGGHTEFADER"
BASE COUNT          454 a          724 c          668 g          435 t
ORIGIN      
        1 gttgccctct tgcttatcgt cctgttcagc gccttttttg cgcccttaat gctgtacgcg
       61 gcggtacgcg cgcggcggcg atacgctgcc gcgagtcgct cgtcctgtcc agttacaagg
      121 aggtcgctat cctcgcctgc accttggagg agccgccgct cgatagcgga gtgcgattcg
      181 attgcgccat catgcagtat gtgtccgaag ctcacgccgc gagcggcgag tgcccgggcg
      241 accaacagac agcgatggca gtcaagcgga tcgcgttccg aacacatcag gcacagtcgc
      301 cgccgtccgg cgtttgcaag caagtgatcg agaccggcct ggaagctcgg tcgttgcgcc
      361 atggcctcgt aatcgacgac gccatcgcaa tagagtgacg gatcgcgtgg ccggccgccg
      421 agctgatcgc cgaaggacag atagtcgata tttgctttgc ccaagagcgg cgcgagattc
      481 ttcgccgaga accacgggca aaatcgcgac acggggattg agcgcacgtc ggcaatggcg
      541 tcgatgccgg taccgcgcag cagcgcgata aagcgctccg ccgaaatatt ggaatgaccg
      601 atagtgaaga gatcgaaggc ggtcatggtt caaattgcat caaagttgcg aattctaagc
      661 gacgtcattg cgagaagcac aggcgacgaa gcaatccgga ccttaagcat gctgccggta
      721 atcgctttac aagcacttca ggttacgtag tgatgaggtg ctgaggtcgc cggggcctgt
      781 catgcgtccc ttgtgctgcg ttctggccgg gttggtattc gccgtcgcgc tgacgaacag
      841 cgccagcgcc gccatcgtcg tcgacatcga tcgttccgtt cagcggatgg cggtgaccgt
      901 cgacggggtg ccgcgctaca actggcgcgt ctccaccgcg cggcgcggct acatcacccc
      961 gcccggcacc tatcacccgg aaatgctggc gcggcattgg ttctcgcgca aatattacaa
     1021 ttcgccgatg ccgcattcga ttttcttcta tggcgacttc gccatccacg gcagctacga
     1081 gatttcgcat ctcggccgcc ctgcctcgca cggctgtgtg cggctcgatc ccggcaacgc
     1141 tgcggtcctg ttcggcctcg tgcagcgcga aggcatgagc accaccacga tcgttattca
     1201 ttagcgttga ctagcgaccg ctccccggcg cggcggcgag aggaagcaaa aaagatcccg
     1261 ttctcgcggg caaacaaaaa agaaggggac cggcgacggc cgatcccctc acacccccca
     1321 acgcttgcct aacctttggt aacaaccggc ggcggcgctt tgcccttgcc gtagtactgc
     1381 gcacagcccg cgatgctgag gagagcgagc accgcggcca gtggtagagc gaacttcttc
     1441 atggaaaata actcccccgc catgaccgct catcctaatg gcgcgccctg gtgcgcctct
     1501 agtgttggaa agacacatac gatcccccgc agcgcgccga caccgttgcg cggaagcggc
     1561 tattttgcgg ccatggaact tgccacaatt aggcgactgc aatgacctcg atcccccgcg
     1621 atctcccatg atcaagaaat ccgggccgta gaatggtgag acctctatac cctaatccta
     1681 aggtacgccc caacggcccg ccccgaagtg gccggccgat gacaagctcc gcgagcctca
     1741 gggtgacggc gcgctgacgt tacgatcggc gcggtgactc cccggtttgg ctcggtaagc
     1801 gtaagataac gcggcggacg acctacccaa ggatctgctc atggccatca ccgtttgtcc
     1861 ggtcacgctc ggttttgtcg ctgagatcgg cgacatcgac ttatcgaccc cgctcgcacc
     1921 ggccgacctt ctggccgtca aagaggcgtt cacaaaatat gccgtgctga tctttcccga
     1981 tcagcatctg tcgcaggatg cgcatctcaa cttcgcgaga aacttcggtc cgctcgagac
     2041 caccatcgcc ctgttccgca aagatgcgaa attacgggtg cgcaaggagt tcgctgacgt
     2101 ctccaatctc gatgccgaca acaaggtgtg gggcgagaac tcgcgacgac gcatgttcga
     2161 gctcggcaat cggctatggc acaccgacag ctcgttcaag cacctaccgg cgcgggcgtc
     2221 gctgctctat gcgcgttcga ttccgccgct cggcggccac accgaatttg ccgacgagcg
     2281 c
//
LOCUS       DATLJT010000533         3267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3707407,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000533 DATLJT010000000
VERSION     DATLJT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707407"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..317)
                     /locus_tag="VM910_12405"
     CDS             complement(27..317)
                     /locus_tag="VM910_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63368.1"
                     /translation="MFEICPTCGQPVVSTDLILPPIKARIFELVRQRPGIDAETLRTL
                     VWEDDPAGGPEDRKVLHVHVHQLNDLLRVHRIQVRGSRSGGYRVQPLRQVTP"
     gene            complement(394..618)
                     /locus_tag="VM910_12410"
     CDS             complement(394..618)
                     /locus_tag="VM910_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63369.1"
                     /translation="MREHLRNDGCIALVDDDRSDEIVVETVVDDFRPVRGASCWAGGA
                     EPRSWSWRRITTDNIEITCHRSHRDLRTSA"
     gene            complement(699..884)
                     /locus_tag="VM910_12415"
                     /pseudo
     CDS             complement(699..884)
                     /locus_tag="VM910_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
     gene            complement(2244..2885)
                     /locus_tag="VM910_12420"
     CDS             complement(2244..2885)
                     /locus_tag="VM910_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63370.1"
                     /translation="MTHSPKELIKNNTSLTPEEVAQLGIRWEQISADVFQIRGPDIGQ
                     RIENVIAHLRQRAELYIEEKPATDGTFDCTAAAKAFLYKLQVIECLLAAAERNGAERN
                     GAERNGASPDLRQAVYYSLLLAINDHQLTVIGNETAIAARQASIKGARTGGRLRSANV
                     RIRNREMALKFLARRGGNLKDTALMAKIGAAKNLKRRASVNAIESGLKDLNRD"
     gene            complement(2889..>3267)
                     /locus_tag="VM910_12425"
     CDS             complement(2889..>3267)
                     /locus_tag="VM910_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63371.1"
                     /translation="KARHVVLTDEGAEFFKGLTAGRAGNEPMFGRQWGVSHQLRPMAE
                     AVIRAKITPAISFHGLRHTWASHAVMNGVPLLVVAKNLGHRDTRMAELHYGHLAPSYI
                     VDTIRARAPRFGMVESSGLRVLR"
BASE COUNT          619 a          975 c          980 g          693 t
ORIGIN      
        1 aaggccgggg tcgatgccaa gcgctttcat ggcgtcacct ggcgtaacgg ctgcacgcga
       61 taaccgccgc tgcgcgaacc gcgaacttgg attctgtgaa ctcgtagtag gtcgttcagt
      121 tgatgcacgt ggacatgaag aactttgcgg tcttccgggc cgccggcggg atcgtcctcc
      181 caaaccaacg tacgcagggt ctcggcatcg atgcctggtc gctgacgaac gagctcgaag
      241 atccgcgcct tgatcggcgg cagaatcaga tcagtcgaaa cgaccggctg gccgcatgtc
      301 gggcagatct caaacacggt gtttttcatc cttcaagaac ttgtcgagct ctgttgctct
      361 gcggcggttg atagtcgttt caccgatgcg tccttacgcc gacgtgcgca agtcgcgatg
      421 actgcgatgg caggtaatct cgatgttatc ggtagtgatc cgacgccagc tccacgagcg
      481 cggttcagct ccaccggccc aacacgaagc gccgcgaacg ggcctgaagt cgtcgactac
      541 ggtctcgaca acgatctcgt cacttcggtc atcgtcgacg agagcgatgc agccgtcgtt
      601 gcgaagatgt tcacgcatgc ggcgcctcct gtagtgagtt tgctcgcctc gaaagggagt
      661 cggaggattc gttgcgggct cgccaagagt tgctggcgca gaaaatcgac cgccgactgc
      721 gcccgcgcgg ccgccgtgaa gattgccctg ggatcgtcct caagtatctc gaggtagtac
      781 ccgatgtagt cggcgtatgg gatgatcccg tcgatcgaga actctgcgca caggaacgct
      841 gcgcccagtt ctgcgacgag ttcttctgcg gcgcgatctc gccggtgatc gccgccgcct
      901 gcttcctgca gcgagctgga cctggcgctc gtgcacggac ttcgtcgtag gcttgatcgg
      961 atcgcagcac ccgtgccttt gggaccggca cgccttcgac tgcgaaggtg acggtctgct
     1021 gccacaaatc agtgcccatg tgactggcct ctttaataat agaaagttag agacaagatg
     1081 gagacaggca ccgattgcct cggcgtgcgg ttttcttctg caaggtcacg ccgagatcgg
     1141 gagcgtccat ttctaggatg acgagctcag gccgccgcct tctagttgtc ggcgccactc
     1201 actggcggat tcagcagtga tcaagttccg cgagccgatt tttattgagc gtgggcctgt
     1261 gcccgcgcgc caaagctgat agagcttgct gcgagatatt ttgtgtgccg agcaaaattc
     1321 ggcgaccgtg tagaccacgg ggcctgccga gtcagcgctc gcgcggggat cgctttgcgg
     1381 agagtggata tgttgggcca ttgctgcggg tccaggtgga aacccccgca tatctagcag
     1441 tgcattcgtg cagggtgccc ccctcgatgc acgaaaagtg aaagcaagta acgacgaggc
     1501 tccgtttcca tgagcccctg cctgctttct cccgccggcg acggcaaccg acccagctgg
     1561 atccaccaaa tcaagcgcac tggtggcccg ccgtgaggct tgaaccgggc gcgcgttttg
     1621 gttaaccaaa aataatgccg agaaagcgca gacgggggcg gccgcgtacg ggccgagatc
     1681 cgatggtcgg cgtgcgtgcc cgcgcgggtg ctcaagaaga tcgacaagct gatggtggcc
     1741 ttgagctttg atcgctcggc cgccatccgc tggctccttg aggctggcct ggagtccggc
     1801 atagcgctca tgctgctgcg tgccagcaaa gcgaggcgtc agtccgacca aatctgccaa
     1861 gttatagcgg cggggctcaa gcatcgaatc gccgtcagcg ccgtgaagcg cgctggcccg
     1921 cgaacgagct tggacgccac cctgcgcgcc caccgtgctg ccgaagaagc tgccgagctt
     1981 cgatctagcc ttgcggacag gatcgcgctg aggcaggcgg ccaaatccag cggtgcggcg
     2041 cctcctacga ctctatcagt ccccacgcct ccgaacgttt tgcagccgcc atcacgaaaa
     2101 ccgcgagatt taaccgcggc ggaggtgagg gctgcggtcg atcgcgctct tgctcgttcg
     2161 gccaaaggga cgtctagctg aagtgcggaa gaacactcgg tgggggcctc ccagggtgcc
     2221 ccctgaatgc acgaaaagtt ggattaatcg cgattcagat ctttaagccc cgactcaatc
     2281 gcattaacgg acgcccttct tttcaaattt tttgcggctc cgattttcgc catcagggcg
     2341 gtatccttca agtttccgcc ccggcgggcc aggaacttga gtgccatttc gcgattgcgg
     2401 attcttacgt tggcggaccg aagcctgccg ccagtgcgcg cacctttaat gcttgcctgc
     2461 cgagctgcga tcgccgtttc gttaccgatt accgtaagtt gatgatcgtt gattgctaac
     2521 aacaggctgt aatagacagc ctggcgaagg tccgggcttg cgccattgcg ctctgcgcca
     2581 ttgcgctctg cgccattgcg ctccgccgcg gcgagcaagc attcgataac ctgcagctta
     2641 taaaggaatg ctttagcagc ggctgtgcaa tcgaacgtgc cgtcggttgc aggtttttct
     2701 tcaatgtaca attccgcacg ctgcctcaaa tgcgctatca cgttttcgat tctctggccg
     2761 atgtccgggc ccctaatttg aaaaacatca gcgcttatct gctcccaacg tatgcctagt
     2821 tgcgcaactt cctccggcgt tagcgacgtg ttgttcttaa tgagttcttt cgggctgtgc
     2881 gtcatagttc acctcaacac gcgtagaccg cttgactcga ccatgccgaa gcgcggcgca
     2941 cgagcacgaa tggtatctac gatgtagctg ggcgccaggt gcccgtagtg caattcggcc
     3001 atgcgcgtgt cgcgatgacc cagattcttc gccaccacca gcaggggcac cccattcatc
     3061 acggcgtggc ttgcccaggt gtggcgcagg ccgtggaagc tgatagcggg cgtgatcttc
     3121 gcccgtatga cggcttcagc catcggacga agctgatgcg aaacgcccca ctgcctgccg
     3181 aacatcggtt cattcccggc gcgcccggca gtgagtcctt tgaaaaattc agcgccttca
     3241 tccgtgagta cgacgtggcg ggctttg
//
LOCUS       DATLJT010000534         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4396451,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000534 DATLJT010000000
VERSION     DATLJT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4396451"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VM910_12430"
     CDS             complement(<1..845)
                     /locus_tag="VM910_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63372.1"
                     /translation="MTAKSCIAHFAGPNATILNSPPLVTSNKARRKYGLPLVTNPDGT
                     PARFDVLRPQRLAAPATVYVEQFSAHPLECDAAELYGPPDGYLDAQGCFHKERQSPAD
                     KPVYEIELRPEDGPYPLPYMARQADLRAWEEECAEPGAPAHRARQVFHPDGARIFEEI
                     DRMGISDKGWGNLISSRVDVDFYRALPPAGYTKGLAAIERTDIGEGDIAPEVRGRHFF
                     PYKPYHLDAHETRGSLAHLTNVVQRVLASGKYHGAIWTQGSPRIEETIYWLNLLLDCT
                     VPVCG"
     gene            complement(1015..1677)
                     /locus_tag="VM910_12435"
     CDS             complement(1015..1677)
                     /locus_tag="VM910_12435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVI63373.1"
                     /translation="MRTLADKVRPEHCGILIVDVQNDFCAEGGAMHREGRDLSQVKAM
                     VPRLARLIEVARAAKVRCIWIRNVYNTGPNWYLSEVWLEHAQRRRQGAYISIPVCEPD
                     AWNSDFYELKPLPEEPIVTKHRYGAFESSDLDLVLRSQGIRTVVMTGVATNVCVETTA
                     RQAFLRDYYVVFSSDCTATYDQAAHDATLRNIDAFFGQVVSAEEIQACWEQEPARLRA
                     VR"
     gene            complement(1692..>2709)
                     /locus_tag="VM910_12440"
     CDS             complement(1692..>2709)
                     /locus_tag="VM910_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63374.1"
                     /translation="EDHPLAPGEQWPHNLKENRMSRSLQVFCSVMVASLAAWIVPQAA
                     AQTKPPPAVIRVVDIPIANFTPLLVARDKGYFAEENLQVSWSPVAQGAVAIEAVFGGS
                     AEVGGSAVFETMVARGNGLDLMFLAPGVRIRSQPPDNSALLVRADNSIRGPADLAGKK
                     VSAGLINSVNYVHMLEWLQKHGVDRSKIEFLEVPFPQMADALFQKRVDAVWNVEPFVT
                     FMTKSGKARVLAYPYQETIPGMEIANYFAKESWIKANPDVARGFRRAIDRATDFMATT
                     SKEERDEWVSKFTGMKLDVVKEVTLPQFSTEFNIPSFQANLDIAVRHKMAKPFDVNVM
                     IFKP"
BASE COUNT          481 a          846 c          855 g          527 t
ORIGIN      
        1 ttaccgcaca ccggcaccgt acagtcgagc agcagattca accaataaat cgtctcctcg
       61 atgcgtgggc tcccttgggt ccagatcgca ccgtggtatt tgccgctcgc cagcacacgc
      121 tgcacgacat tggtgaggtg agcgaggctt ccccgcgtct catgcgcgtc gaggtgatag
      181 ggcttgtacg gaaagaaatg gcgcccgcgc acttccggag cgatgtcacc ttcgccgatg
      241 tcggtccgct ctatggcggc aaggcctttg gtgtatcccg ccggcggcag cgcgcggtag
      301 aaatcgacat cgacgcggga ggagataaga ttgccccagc ccttgtcgct gatgcccatg
      361 cgatcaatct cctcgaaaat gcgcgcgccg tcgggatgaa agacctggcg cgcccgatgc
      421 gccggggcgc ccggctcggc gcattcctcc tcccaggccc gcaggtccgc ctggcgcgcc
      481 atgtagggca agggatacgg cccgtcctcg ggccgaagct cgatctcgta gaccggcttg
      541 tcggcggggc tttgccgctc tttgtggaag cacccttgcg cgtcaaggta gccatcgggc
      601 ggcccgtaga gttcggcggc atcgcactcg agcggatgcg cgctgaattg ctcgacatag
      661 accgtcgccg gcgccgccag tcgttgcggc cgaagcacgt cgaagcgggc cggggtccca
      721 tccggattgg tcaccagcgg cagcccgtac ttccgccgcg ccttgttgct cgttaccagc
      781 ggcggactgt tcaggatggt ggcattaggc ccggcgaaat gggcgatgca cgactttgcc
      841 gtcatagtgg aatgacccct cccaacttac gggactcgtt tctcttaaga tgattgcgtt
      901 ggcaagatgg cgcgctccgg gctcagccgt agtaatggca ggtgagcgcg cacgggcaag
      961 catggtccgc tatgcacgga ccatttgtgt cagccgagac accggatcga gcgttcagcg
     1021 cactgcgcga aggcgcgcgg gctcctgctc ccagcaggcc tggatctcct cggcgctgac
     1081 cacttggccg aagaacgcat cgatgttgcg caatgtcgcg tcgtgcgcgg cctgatcgta
     1141 ggtcgccgtg cagtcgctgc tgaagacgac gtagtagtcg cgcaggaatg cctgccgtgc
     1201 cgtcgtctct acgcatacgt tggtggccac tccggtcatg accacagtac ggatgccctg
     1261 cgagcgcagc accagatcga gatcgctgct ttcgaatgcg ccgtagcggt gcttcgtcac
     1321 gatcggttcc tcgggtagcg gcttgagctc gtagaaatcg ctgttccatg cgtccggctc
     1381 gcagaccgga atgctgatat aggcgccctg ccgacgccgt tgcgcgtgct ccagccagac
     1441 ctccgaaaga taccagttgg gaccggtgtt gtagacattg cggatccaga tgcagcgcac
     1501 cttcgcggcc cgcgccacct cgatcaggcg cgcgagccgc ggcaccatcg ccttgacctg
     1561 cgacaggtcg cggccttcgc gatgcatggc cccgccttcg gcgcagaagt cattctgcac
     1621 gtcgacgatc aggatcccgc agtgctctgg acgaaccttg tcggcgagcg tcctcaacat
     1681 ggctcctctc ctcagggctt gaagatcatg acgttgacgt cgaacggctt ggccatcttg
     1741 tgccgtacgg cgatgtcaag attggcctgg aaactcggga tgttgaactc ggtcgagaac
     1801 tgcggaaggg ttacttcctt gaccacatca agcttcatgc cggtaaactt cgacacccac
     1861 tcgtcccgct cctccttgga ggtggttgcc atgaagtccg tggcgcggtc gatggcgcgg
     1921 cggaagccgc gcgccacatc cggattggcc ttgatccagc tttccttggc gaaatagttg
     1981 gcgatctcca tgcccgggat ggtctcctgg tacggatagg cgaggacgcg cgccttcccg
     2041 gatttggtca tgaaggtgac gaacggctcg acgttccaga cggcatctac ccgcttctga
     2101 aacaaggcgt ccgccatctg cgggaatgga acttcgagga attcgatctt gctgcggtcg
     2161 acgccatgtt tctgcagcca ttccagcatg tgcacatagt tgacgctgtt gatgagacca
     2221 gcagaaacct ttttgccggc gagatcggca gggccgcgga tgctgttgtc ggcgcgcacc
     2281 aggagcgcag aattgtcggg cggctgtgac ctgatgcgga cgccaggagc gaggaacatg
     2341 aggtcgaggc cgttgccgcg ggcgaccatc gtttcgaaca ccgcgctgcc gccgacctcg
     2401 gcactgccgc cgaacaccgc ctcgatcgcc accgcgccct gcgcgaccgg agaccagctg
     2461 acctgcaggt tttcctcggc gaaatagccc ttgtcgcgcg caacaaggag cggcgtgaaa
     2521 ttagcgatcg gaatgtcgac cacgcgaatg acggcgggcg ggggcttggt ttgcgctgcc
     2581 gcctgcggaa cgatccatgc cgccagcgag gcgaccataa ccgagcagaa tacttgcaac
     2641 gatcgggaca tccgattctc cttcaggtta tgcggccact gctctcctgg tgccagcgga
     2701 tgatcttcg
//
LOCUS       DATLJT010000535         1690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3937053,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000535 DATLJT010000000
VERSION     DATLJT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3937053"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1690
                     /gene="pepN"
                     /locus_tag="VM910_12445"
     CDS             <1..>1690
                     /gene="pepN"
                     /locus_tag="VM910_12445"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HVI63375.1"
                     /translation="FELEIETVLDPTNNTKLMGLYRAGSTYCTQCEAEGFRRITYFLD
                     RPDVMAVYTTRIEAEKKEASVLLANGNLVDHGDIAGTSRHFAVWHDPFPKPSYLFALV
                     GGNLGCVTDSFTTMSGRNVTLAIYVEPGKEDRCSYAMDSLKRAMRWDEVTFGREYDLD
                     IFMIVAVSAFNMGAMENKGLNVFNDKYVLASPATATDGDYAGIEAVIAHEYFHNWTGD
                     RITCRDWFQLCLKEGLTVFRDQEFTADQRSCAVERISDVRELRASQFVEDAGPLAHPV
                     RPRLYHEINNFYTATVYQKGAEVVRMIKTLLGAPAFRAGMDLYFTRHDGQAATVEQFV
                     QCFADVSGRDMRQFMRWYSQAGTPEIKVAPHYDSAARSLRLEVEQIVPPTPGQPNKEP
                     MTVPLALGLVGRNGDDLPLLLDGRPLSCGVIELTESNQSFAFNDVPERPALSLNRGFS
                     APIKLTASIETDELRFLAAHDRDPFNRWQAVQTLAMTLLKSNVATLRSGGAPRLDQGL
                     MDALRAILKDTALEPAFIALTLSPPSEADIAREIGSDIDPDAVYAARRALRAATG"
BASE COUNT          334 a          532 c          493 g          331 t
ORIGIN      
        1 ttcgaactcg aaatcgagac cgtgctcgat ccaacgaaca acaccaagct gatggggctc
       61 tatcgcgcgg gatcgactta ttgcacgcaa tgcgaggccg aaggatttcg ccggatcacc
      121 tacttcctcg atcgtcccga tgtcatggcc gtatacacga cacggattga agcggagaaa
      181 aaagaggcca gcgtccttct ggcgaacggc aatcttgtcg accacggcga catcgcaggt
      241 acgtcgcggc atttcgccgt ttggcacgat ccttttccga agccgtcata tctgttcgcg
      301 ctggtcggcg gtaatctcgg ctgcgtcacg gacagcttca ccacaatgtc cggtcgcaac
      361 gtcacgctcg ccatttacgt cgagcccggc aaagaagacc ggtgtagtta tgcgatggac
      421 agcctcaagc gagcgatgcg ctgggacgaa gtcacgttcg ggcgtgaata cgatctcgat
      481 attttcatga ttgtcgccgt ctccgcgttc aatatgggcg caatggaaaa caagggactc
      541 aacgtcttca acgacaaata cgtgcttgcc tcgcctgcta cggcgaccga tggcgattat
      601 gccggcatcg aggccgtaat cgcccacgag tattttcaca attggacagg cgatcgcatt
      661 acctgccgtg actggtttca gctttgtctg aaggaaggct tgaccgtctt ccgcgaccag
      721 gaattcacgg ccgaccaacg ctcgtgcgcg gtcgagcgca tcagcgatgt gcgcgagctg
      781 cgtgcatctc agttcgtcga ggatgcgggg ccgctggcgc atccagtgcg gcccaggctc
      841 tatcacgaga tcaacaattt ctacaccgcg accgtctacc agaagggcgc cgaagtcgtg
      901 cgcatgatca agacgctgct cggcgcgcca gccttccgcg ccggcatgga cctctacttc
      961 acacgccatg acggccaagc ggcgacggtc gagcagttcg tacaatgttt tgccgacgtc
     1021 agcggccgcg acatgcgcca gttcatgcgc tggtattcgc aagccggcac gccggagatc
     1081 aaggtggcgc cgcattacga ttccgctgcg cgcagcctgc ggcttgaggt ggagcagatc
     1141 gtgccaccga cgccggggca accgaacaag gagccgatga cggttccgct tgcgcttggc
     1201 ctggtcgggc gcaacggcga tgatctgccg ctattgcttg atggccggcc gctctcgtgc
     1261 ggcgtaatcg aactgaccga gtcgaaccaa tcgtttgcgt tcaacgacgt gcccgaacgt
     1321 cccgcgctct cgctcaatcg cggcttttcc gccccgatca agctgaccgc ttcgatcgaa
     1381 accgacgagc tgcgcttcct ggccgctcac gatcgcgatc cgttcaaccg atggcaggcc
     1441 gtgcagacat tggcgatgac attgctcaag agcaatgtcg ctacgctacg ctcgggtggc
     1501 gccccgcgcc tcgaccaagg actaatggat gcgctccgcg caattctcaa agataccgcg
     1561 cttgagccgg ccttcatcgc cttgacgctg tcacctccga gcgaggccga tatcgcccgc
     1621 gaaatcggct ccgacatcga ccctgatgcg gtctatgcgg cgcggcgcgc cttgcgcgcg
     1681 gcgaccggcc
//
LOCUS       DATLJT010000536         1886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3248546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000536 DATLJT010000000
VERSION     DATLJT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..966
                     /locus_tag="VM910_12450"
     CDS             91..966
                     /locus_tag="VM910_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63376.1"
                     /translation="MLDFVQQLVSGIALGCVYGLIALGFVLVYKATEVVNFAQGDLMM
                     LGGFFAFTFIGILGLNYWIGFAAAVLAMAAFGVLAERLVVRPILGYPQFSIIMATIGL
                     GYFLRSVAGMIWGTDDLKIETPFSNGVLRLGSLVLAYDKLSVIAATIMLCAAFYLFFN
                     KTALGTAMRASSENMLAAYYMGIPVKRVVSIVWAISAAVATCAGVLLAPITFIHSNVG
                     LVLGLKAFPAAVLGGFGSIPGAVVGGVLIGVIESMAGFYLPEGWKDVAPYVVLLLVLL
                     LRPEGLFGLPMRKKV"
     gene            976..>1886
                     /locus_tag="VM910_12455"
     CDS             976..>1886
                     /locus_tag="VM910_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63377.1"
                     /translation="MRFLFKTDYEDDIKLFPHSGYVWSYGTLMALLVIAPYVLSSYLV
                     SQLVFVCIYATVGVALLILTGFTGQASLGHAAFLAIGAYTAAYLQQFNVPFPVYFLAA
                     GLLTGVIGAIVGFPALRLQGIYLVIATISFALIVEEILARWESVTHGNEGMRIKTIQL
                     FGTQVPRDSPAFYFLCLAVLVLTIVGSLNLLRSPTGRAFIAIRDSETAARSMGINVAL
                     YKVKSFAISATITGFAGVLFAHKLSFISPEMFTLQLSIEFIIVILIGGTFSLHGAVLG
                     AIFIVMIDPFLTYLKDDVPGMIGNLAAA"
BASE COUNT          294 a          594 c          555 g          443 t
ORIGIN      
        1 gtgacgaatg tgcggtcctt ccggtagccg gaaggacgga gatgcataaa ggcgaccaaa
       61 gagttgccgc gcatccgggg agggaaacaa atgctggact tcgttcagca gttggtgagt
      121 ggcattgcgc ttggctgtgt ctatggcctg atcgcgctcg gctttgtcct tgtctacaag
      181 gcgaccgaag tcgtgaattt cgcccagggc gacctgatga tgctgggcgg cttctttgcc
      241 ttcaccttca tcggcattct gggcctcaat tactggatcg gctttgccgc cgcggtcttg
      301 gcgatggcgg ccttcggcgt gctggccgag cgtctggtcg tgcggccgat cctcggctat
      361 ccgcagtttt ccatcatcat ggcgaccatc gggctcggct atttcctgcg ctcggtcgcc
      421 ggcatgatct ggggaaccga cgatttgaag atcgaaacgc cgttcagcaa cggcgtcctg
      481 cggctgggta gcctcgtgct cgcctatgac aagctctcgg tgattgccgc aacgatcatg
      541 ctgtgcgccg ccttctatct cttcttcaac aagaccgcgc tgggcaccgc catgcgggcc
      601 agttcggaga acatgctcgc cgcctattat atgggcatcc cggtcaagcg cgtggtgtcg
      661 atcgtctggg cgatcagtgc cgcagtcgca acctgcgccg gcgtgctgct cgcaccgatc
      721 accttcatcc attccaatgt cggcctggta ctcggcctga aggcgttccc ggccgcggtc
      781 cttggcggct tcggctcgat acccggcgca gtcgttggtg gcgtcctgat cggcgtgatc
      841 gagagcatgg ccggcttcta tctgcccgaa ggctggaagg acgtcgcgcc ctatgtcgtg
      901 ctgctgctcg tcctgctgct caggcccgaa ggcctgttcg gccttcccat gcgcaagaag
      961 gtttagggga gcgccatgcg gttcctgttc aagaccgact acgaagacga cataaagctg
     1021 tttccccact ccggctatgt gtggtcctac ggcacgttga tggcgctgct tgtcatcgcg
     1081 ccctacgtcc tctccagtta tctcgtcagc cagctcgtct tcgtatgcat ttacgcgacc
     1141 gtcggcgtgg cgcttctgat cctgaccgga tttaccggac aggcctcgct cggacacgcc
     1201 gcattccttg cgatcggcgc ctacacggcc gcgtatttgc agcaattcaa cgttcctttc
     1261 ccggtgtatt tcctcgccgc cggtctgttg accggtgtga tcggcgccat cgtcggcttc
     1321 cctgcgctga ggctgcaagg catttacctc gtcatcgcta cgatctcctt cgccttgatc
     1381 gtcgaggaaa ttctggcgcg ctgggagagc gtgacgcatg gcaacgaggg catgcggatc
     1441 aagaccatcc agctattcgg cacgcaggtg ccgcgcgaca gtcccgcctt ctatttcctg
     1501 tgccttgcgg tgctggtgct gaccatcgtc ggttcgctca atctgctgcg ctcgcccacg
     1561 ggccgcgcct tcatcgccat ccgcgacagc gagaccgcgg cgcgcagcat gggcatcaac
     1621 gtcgcgctct ataaggtgaa atcgtttgcg atctctgcaa cgatcaccgg ctttgccggc
     1681 gtgctgttcg cccacaagct ctccttcatc agccccgaga tgttcaccct ccagctctcg
     1741 atcgaattca ttatcgtgat cctgatcggc ggcacgttca gcctgcatgg cgcggtcttg
     1801 ggggcgatct tcatcgtcat gatcgatccg ttcctcacct acctgaaaga cgacgtgccc
     1861 gggatgatcg ggaatctggc tgccgc
//
LOCUS       DATLJT010000537         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_491106,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000537 DATLJT010000000
VERSION     DATLJT010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491106"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..556)
                     /locus_tag="VM910_12460"
     CDS             complement(170..556)
                     /locus_tag="VM910_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63378.1"
                     /translation="MQWIRVPVSRCGHFTSPALASIALLVTLTGELHAQGKGMRLWNL
                     TTSTISGFQLSPAGKNEWGPNQTLNDKDKEVDHDERLRITGVEPGRYDARVSYRDSRQ
                     CFVRDIEIKADAIFSISDKDLKDCNK"
     gene            complement(586..1371)
                     /locus_tag="VM910_12465"
     CDS             complement(586..1371)
                     /locus_tag="VM910_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVI63379.1"
                     /translation="MKRPIGTAVLVLAVTVVAAAAQDMMRNLDLSSPDMVSAEMTRAD
                     VEAALGAAAPTDFTGKKLSGLDLSGLDLSGVNFRAARLNKTKLAGAKLDHAILDQAWL
                     LDADLTGADLRGASLFASQMGRARLDGADLSGARVAADLTRASLVGASIADAHLGADM
                     RNQSMGLMRAVLKSAKLDRINARGADLSRVDLEFASMKGADLTGASLKNAQLGGADLS
                     GATITDADFDGADLASTTLIAPIGLDRARNFDRARNRDRLIKQ"
     gene            complement(1430..2137)
                     /locus_tag="VM910_12470"
     CDS             complement(1430..2137)
                     /locus_tag="VM910_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI63380.1"
                     /translation="MQLEVHVASKAFKNAAGERHEVLEDIAFALKPGETGVLIGPSGC
                     GKTTLLRIVAGLDHDYQGHVSRPPQTRIGMVFQEPRLLPWRSVEQNLRIAAPQADEAK
                     LSALFAALELNAHRSHFPGELSLGLARRVAVARALAVEPDLLILDEPLASLDDALATR
                     LRGQIASLVESRPMVTLMVTHDLEDAACLGDRLFFLSARPARILDVVPICAPRRLRGE
                     AEIALIKAEIARRATGE"
     gene            complement(2127..2861)
                     /locus_tag="VM910_12475"
     CDS             complement(2127..2861)
                     /locus_tag="VM910_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI63381.1"
                     /translation="MRLLSLALFIVTWWVASLLAGSAKLPPPPAVLAAVITEARSGAL
                     FVNLGATLARVALAFALAMTLGVAIGYWMGRVRIANRLGDPWLILLLNLPALVVIVLA
                     YIWAGLTEAAAIAAIAINKLPTAIVTLREGTRALDPALDEMATAFAFTRGRAFRHVVL
                     PQLAPYIAAAARSGLALIWKIVLVAELLGRPNGVGFEIGVAFQLFDIPLLLAYALSFG
                     AVVLVIETVLVQPFEVKLSRWRPRAA"
     gene            complement(2866..>3481)
                     /locus_tag="VM910_12480"
     CDS             complement(2866..>3481)
                     /locus_tag="VM910_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63382.1"
                     /translation="DLAGRRLAVAGGPLDKSWLLLRASLRQSGIDLKTASDIVYGAPP
                     LLAEKAASGEMDAVLNYWNFCAALEAKGFRRIADIEDILPKLGAKGRTAMIGYVFDEE
                     WGNAHPELVSRFIAMTRAAKEILSSSDAEWERIQPLTGASDVAKLRALRDHYRKGIPR
                     RSIADEEADARVLYRVLATVGGRELVGPAEELDPGTFYHALPGE"
BASE COUNT          675 a         1124 c         1096 g          586 t
ORIGIN      
        1 atccagcggt tctttgcagc aatgcgcgat ggccgcgccg ccctatatcg cacagtggat
       61 cggccagcat ccgaactgga ccgcggtcaa gtggcgctgc gagtatccgc acagcaagga
      121 aaaagccgac gccggcaagg ccacaccgat cggctagctt gcacacgcgc tacttgttgc
      181 agtccttcag atccttgtcg gagatcgaaa atatggcgtc cgctttgatc tcgatgtcgc
      241 ggacaaagca ttgcctcgaa tcgcggtagc tgaccctggc atcgtaacgt cccggctcca
      301 cgccggtgat gcgcaagcgc tcgtcgtggt cgacttcctt gtccttgtcg ttcagcgtct
      361 ggttgggtcc ccattcattc ttgccggcgg gcgaaagctg aaaaccggag atggtcgatg
      421 tggtcaggtt ccacaagcgc atgccctttc cctgcgcgtg aagctcgccg gtcaacgtca
      481 cgagcaacgc aatcgacgcc agtgccggcg acgtgaagtg cccacatcgg gagaccggca
      541 ctcgtatcca ttgcatgaca atcctcgtca acggctccgg aaccgtcatt gcttgatcaa
      601 ccggtcgcga ttccttgccc tgtcaaaatt tctggcgcgg tcgaggccaa ttggcgcgat
      661 caatgtcgtc gatgcaagat cggcaccatc gaaatctgca tcggtgatgg tggcgccgct
      721 caggtcggct cctccgagct gggcattttt cagcgaagcg cccgtcaaat cggccccctt
      781 catcgaagca aattcgagat cgacgcgaga cagatccgcg ccgcgcgcgt tgatgcgatc
      841 gagcttggcg gacttcagca cggcgcgcat cagacccatc gactggttac gcatgtctgc
      901 gcccagatgt gcgtccgcaa tcgaagcccc gaccaggctt gcgcgggtca ggtccgcggc
      961 gacgcgcgcg ccggagagat cggcgccgtc cagacgcgct cgccccatct gggaggcgaa
     1021 cagactcgcg cccctcaggt ctgcgccggt caggtctgca tcaagcaacc aggcctggtc
     1081 gaggatcgca tgatcgagtt tcgctcccgc gagcttcgtc ttgttaagac gggccgcacg
     1141 gaagttcacg ccggaaagat cgagccccga aagatcgaga cctgagagtt tcttgccggt
     1201 aaaatcggtg ggtgccgcgg cacccagcgc tgcctctacg tcggcacggg tcatttcggc
     1261 cgaaaccatg tcgggcgacg acaggtcgag gttgcgcatc atgtcctgtg ccgctgccgc
     1321 aaccacggta acggcgagga cgagcaccgc ggtgccgatg gggcgcttca tcgccaactc
     1381 cacggccgtc gttcctagca caacccccca gctctggcta tcggacgtct cactccccag
     1441 tagcccgtcg cgcaatctct gccttgatca aagcgatctc ggcttcgcca cgtaaacggc
     1501 gcggcgcgca aatcgggaca acgtcgagaa ttcgcgctgg tcgcgcggac agaaagaaca
     1561 gccggtcgcc aagacaagcc gcatcttcca gatcgtgggt gaccatgagt gtcaccatgg
     1621 gccggctttc cacgaggctc gcgatctggc cgcgcaagcg cgtggcgagg gcatcgtcga
     1681 gcgaagccag cggctcgtcg aggatcaata aatccggttc gacggcgagc gcgcgtgcga
     1741 ccgcgacgcg ccgggcaagc ccaagcgaca actcgcccgg aaaatggctg cgatgggcat
     1801 tcaattccag cgcggcgaac agcgccgaaa gctttgcttc atcagcctgc ggcgcggcaa
     1861 tccggaggtt ttgttcgaca gagcgccagg gcaacagtcg cggctcctga aacaccatgc
     1921 cgatccgcgt ctgcggcggc cgcgagacgt gcccctgata gtcgtgatcc agaccggcga
     1981 cgatccggag cagggtggtc ttgccgcagc ccgacggtcc gatcaagacg ccggtctctc
     2041 ccggcttgag cgcgaacgcg atatcctcga gcacctcgtg ccgttcgccg gccgcgttct
     2101 tgaacgcctt gcttgcaaca tggacctcaa gctgcacggg gacgccaccg ggacagcttg
     2161 acttcgaagg gttgaaccag gacggtttcg atgacgagca cgacggctcc aaagctcagc
     2221 gcataagcaa gcaggagcgg gatatcgaag agctggaagg ccacgccgat ttcgaaaccg
     2281 acgccgttcg gccgccccag cagctccgca accaggacga tcttccagat cagcgcaaga
     2341 ccggatcgcg cggcggcggc gatatagggg gcgagctgcg gcaggacgac gtgccggaaa
     2401 gcgcggccac gggtgaaggc aaaggcggtt gccatttcgt ccagcgccgg gtcgagcgcg
     2461 cgggtgcctt cgcgcagtgt gacgattgcg gtcggcagct tgttgatcgc gatcgctgcg
     2521 atcgcggcgg cctcggtcag cccggcccag atataggcaa gcacgatgac gacgagcgcc
     2581 ggcagattga gcagcaggat cagccaggga tcgccgagac ggttggcgat ccgcacgcga
     2641 cccatccagt agccgatggc gacgccgagc gtcatcgcga gcgcgaaggc aagcgcgacg
     2701 cgcgccagcg tcgctccgag attgacgaag agcgcgcccg atctggcttc cgtaatgacg
     2761 gccgcgagca cggcaggggg aggcggcagc ttggcgctgc ccgcgagcag ggaagcaacc
     2821 caccaggtga cgatgaacag ggccagggac aacaggcgca tcggctcatt ccccgggaag
     2881 ggcgtgatag aaggtgccgg gatcgagctc ttccgccggc ccgacgagct ctcggccgcc
     2941 gaccgtcgcc agcacgcgat agagaacgcg tgcgtcagct tcttcgtccg caatcgaacg
     3001 ccgcggaatt cccttgcggt aatggtcccg gagcgcgcgc aactttgcga cgtcggaagc
     3061 tccagtcagc ggttgaatgc gctcccactc cgcgtccgac gatgacagaa tctccttcgc
     3121 ggcgcgggtc attgcgatga agcgcgaaac cagctccgga tgtgcgtttc cccattcctc
     3181 gtcgaagaca tagccgatca tcgcggtgcg gcccttcgcg ccaagctttg gcagaatgtc
     3241 ctcgatgtcg gcgatgcgac gaaagccctt cgcttccagc gcggcgcaga aattccagta
     3301 attgagcacc gcgtccattt caccgctcgc cgccttttcc gccaacagcg gcggcgcgcc
     3361 atagacgata tcggatgccg ttttcagatc gatcccgctc tgccgcagcg aggctcgcag
     3421 cagcagccag cttttgtcga gcgggccacc ggcgacggca agcctgcggc ccgcaaggtc
     3481 c
//
LOCUS       DATLJT010000538         1889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1180775,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000538 DATLJT010000000
VERSION     DATLJT010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180775"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VM910_12485"
     CDS             <1..113
                     /locus_tag="VM910_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5076 domain-containing protein"
                     /protein_id="HVI63383.1"
                     /translation="AYTEEEAMARILDMFEAEIERPTDMGSTTPRSQKGH"
     gene            116..568
                     /locus_tag="VM910_12490"
     CDS             116..568
                     /locus_tag="VM910_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HVI63384.1"
                     /translation="MAIESYHYEFMAEAIREAEIGISEGGLPIGAVLTRNNRIIARGH
                     NRRMQEKNVILHGEMSCLRDAGLISFHDTVMYTTLSPCSMCAGAIGLFKLSLLVIGES
                     ETFPGSKDILSKFNIPFIDLSDERSINMMKNWRSNPANERVWQGDIGN"
     gene            582..>1889
                     /locus_tag="VM910_12495"
     CDS             582..>1889
                     /locus_tag="VM910_12495"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HVI63385.1"
                     /translation="MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKA
                     TMEVEAVDEGTIAKILVPEGTQDVPVNDVIAVLAGDGEDVKAASAGAGAAKPAPKPAE
                     APAPKPTPVAAATAPAASAPPPAVAAPPIVPEASPAPRGNGQGRIFSSPLARRLAKEA
                     GVELGRIMGTGPHGRIIARDVEQAKSGRGLKAPAAAPSAGPALAPSMSDKQILALFEP
                     GSYEIVPHDGMRRTIAQRLTASVQNVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKK
                     PLYKISVNDFVIKAMAVALQQNPNCNVSWTDSGMLKHKHSDIGVAVAMPGGLITPIIR
                     KAETKTLSTISNEMKDFAARARARKLKPEEYQGGTTAVSNLGMYGINHFTAVINPPHA
                     TILAVGTSEERPVVRGGKIEVASMMSVTLSCDHRAIDGALGAEL"
BASE COUNT          408 a          577 c          596 g          308 t
ORIGIN      
        1 gcgcctacac cgaggaagag gcgatggcgc gcattctcga catgttcgaa gccgagatcg
       61 aacggccgac cgacatgggc tccacgacgc cgcgatcgca aaaaggtcac tgactttggc
      121 aatcgagtcc tatcattacg agttcatggc cgaggccatt cgcgaggcag aaatcggcat
      181 ctccgaaggc ggcttgccga tcggcgcggt cttgacgcgg aacaacagga tcatcgcgcg
      241 cggtcataac cggcggatgc aggaaaagaa cgtcatcctg cacggtgaaa tgagttgcct
      301 gcgtgatgcc gggctgatct cgttccacga caccgtgatg tacaccacgc tgtcaccgtg
      361 ctcgatgtgt gcgggtgcga tcggactgtt caagttgtcg ctgctggtca tcggcgagtc
      421 cgagaccttt ccgggaagca aggacattct gagcaagttc aacatcccct ttatcgatct
      481 ttcggacgag cgatcgatca acatgatgaa gaactggcgg tcgaaccccg ccaacgagcg
      541 cgtctggcaa ggcgacattg gcaattgatt aagaggtcgg tatgcccatc aacattctga
      601 tgcccgcgct gtcgcccacg atggagaagg gcaacctggc caaatggctc aagaaagagg
      661 gcgacaaggt caagtcgggc gacgtcatcg ccgaaatcga gaccgacaag gcgaccatgg
      721 aggtcgaggc ggtcgatgag ggcaccattg cgaaaatcct ggtgccagag ggtacgcagg
      781 acgtgcccgt caatgacgtg atcgcggtgc tggccggcga tggcgaggat gtgaaggcgg
      841 caagcgccgg agcgggggcg gcgaaacctg cacccaaacc agccgaagcc cctgcaccga
      901 agccaacccc cgtcgcagcc gcgacggcgc ctgcagcatc cgcacctccg cctgcagttg
      961 ccgcgccgcc aatcgttccc gaagcgtcgc cggcgccgcg gggcaacggc cagggtcgca
     1021 ttttttcttc gccgctggcg cgacgccttg ccaaggaagc cggcgtcgag ctcggccgta
     1081 tcatgggtac cgggccgcac ggccgcatca tcgcgcgcga cgtcgaacag gcgaagtcgg
     1141 gtagaggact gaaagcgcct gcagctgcgc catcggctgg acctgcgctt gcgccatcga
     1201 tgtcggacaa gcagattctg gcgttgttcg agccgggcag ttacgagatc gtgcctcatg
     1261 atggcatgcg ccgcacaatt gcgcagcgcc tgactgcttc agtgcagaac gtcccgcact
     1321 tctatctcac catcgactgc gatatcggca agctgctcgc tgcgcgcgag gagatcaacg
     1381 cggcggcacc caaggacaag gagaagaagc cgctctacaa gatctcggtg aacgacttcg
     1441 tcatcaaggc gatggcggtg gcgctgcagc agaatcccaa ctgcaatgtg agctggaccg
     1501 acagcggcat gctcaagcac aagcattccg atatcggcgt agccgtggcg atgcctggcg
     1561 gattgatcac cccgatcatc cgcaaagcgg agaccaagac gctttcgacc atctccaacg
     1621 agatgaagga ctttgccgcg cgcgcccgcg cccgcaagct gaagcccgag gaataccagg
     1681 gcggcaccac ggcggtctcc aatctgggca tgtacgggat caatcacttc actgcggtga
     1741 tcaatccacc gcacgcgacg atccttgcgg tcggcaccag cgaggagcgc ccggtggtgc
     1801 gcggcggcaa gatcgaggtc gcaagcatga tgagcgtgac gctgtcctgc gatcaccgcg
     1861 caatcgacgg cgcgctcggc gccgaactg
//
LOCUS       DATLJT010000539         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3937146,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000539 DATLJT010000000
VERSION     DATLJT010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3937146"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VM910_12500"
     CDS             <1..244
                     /locus_tag="VM910_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63386.1"
                     /translation="RRLGWRPEFCWERLTRWAERHGGVRLLERRPMPPLGHFSLIRFE
                     RLARGGDGGRAGPDSGGRFPLKELHNLASFCQNNNL"
     gene            complement(930..1724)
                     /locus_tag="VM910_12505"
     CDS             complement(930..1724)
                     /locus_tag="VM910_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63387.1"
                     /translation="MVKRVRLNSEELASGLRGPEDKSSHRAGKHGNALKHGVFAKIVI
                     LPWEDPEQFRILHTAVIAEWNPVGPTEEDAVFTIAKSMWRKRRMQLFLENDMERCSTY
                     PDHVAYREAHMLRSFCEILESSANKPENFESCFRLLSAENRDRLRRKFPRHDFQSTSE
                     WIRAIQNEVTSVLLPEAERFDRSVEVLISRDAGFFTQEVVKHELAVDERLDAMIDRAV
                     KRLMQAKAYKQMLGSTSANGRGDQFANNKPNGLPKSSIEKPGRQSG"
     gene            complement(1914..2108)
                     /locus_tag="VM910_12510"
     CDS             complement(1914..2108)
                     /locus_tag="VM910_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63388.1"
                     /translation="MIRMSRSSEHTVSTVSSPRHQASDEIGDTNYRSDLVDPCSIGQG
                     WLAAADDADGADANAAFSVV"
     gene            complement(2323..3900)
                     /locus_tag="VM910_12515"
     CDS             complement(2323..3900)
                     /locus_tag="VM910_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63389.1"
                     /translation="MLIDIAPCDDLFHAAAGTAFADITLDGHRETWPIRSKRFRGWPR
                     RCYYQATGGAASAAEIRSALDLLEARAQFDGPERAVHVRIAEHAGHIYLDLADEQWRA
                     VDIGPDGWRMIACPPVRFRRPAGMLPLPVPQQGGSIEPLHSFLNLASRDDFVLIVAWL
                     LAALRPNGPYPLLAISGEQGSAKTVLSKLLKALIDSNTAPVRSLSREERELMITANNG
                     YLLAFDNLSGLPVWLSDALCRLASGGSFAVRQLYTDDEEVLFEAARPILLNGIEDLIS
                     RPDLGDRAIFLTLAPIGEAQRRPENELWREFEIVRPRMLGALLDAVVHGLRALGRIHL
                     DRLPRMADFALWAAACETALWPPGTFARAYAANRRTAVEGIIDADPVAACVRDIMAER
                     GSWTGSAAELWRAGAHRSNHDLLNECTGWPKNPRTLAGRLRRAQTFLRALGIDIAFSR
                     EGRAGSRIIRIHATQENIVTTVSSVCDQPPPRRAGDVCDDDPRSDLVGRYPMGPVCVT
                     TADDADGADANAGLHVG"
BASE COUNT          764 a         1184 c         1198 g          813 t
ORIGIN      
        1 acggcggttg ggctggcggc cggaattctg ctgggaacgg ctgacgcgat gggccgagcg
       61 ccatggcggc gtgcgcctgc tcgaacgccg ccccatgccg ccgctcggac atttctcgct
      121 gatccgcttc gagcggctgg caaggggtgg cgacgggggg agggcggggc cagatagcgg
      181 cgggcggttc ccattgaaag aactgcataa tttggcttca ttctgtcaga ataacaattt
      241 gtagctgtgt actagagcag ccgcaatcgt ccgaaacaac ggccgccgtt tgcgtcaata
      301 ccgtcagcat cgtctgcagc tgtgagaggc ctggccaatt gaacaggcgc catcggcggg
      361 ttgaggttgt tttgacccca atcgtggttc ctggcggacg ctgctaacgg tgctgacggt
      421 acgttcggca tcgtgctcac gctgatcatt cacgttcgtg attggcctcg cgagagaacg
      481 taacctcgat ccagccacgc gaatgattca ctcgccttgt gcatgggccg cctccatcgc
      541 cagcaggtcg aagctctccg cgccggattt tgagcggttg gctcgcacca tgcccgatgc
      601 gcttcggcga tcagcgtgtg aactcttgca tctcgacatc agcgtcaacc ccaccgatct
      661 cgcgcacaag cgcctcgacc tcttccgaca gcgcgggatc aatccatgca aggcgcgttt
      721 gcgctgatcg gggtgtggca cggtgctgta atacaactta tgcgtgtggg gcgcacaacc
      781 ggcatgcgaa accgcgccaa tgtgcacacg cgagtatctc gcggcgcaca ccaagtactt
      841 cgcggaggat cgaaatcgtc acggccctgc cgcgagaacc acccgcgaga tcggcccttt
      901 aagttcgcgt tgctgctggc ttcttgagct tatcccgact ggcggcccgg tttttcaatg
      961 ctggacttgg gcaatccatt gggcttgttg ttagcgaact gatcgcctct tccatttgcg
     1021 gaagtgctgc cgagcatttg tttataggcc tttgcctgca tcagacgttt gacggctcgg
     1081 tcgatcatag cgtcgaggcg ttcgtccact gcaagttcgt gcttaacgac ctcctgcgtg
     1141 aaaaaacctg cgtctcgtga aatcaatact tcaactgaac ggtcaaaacg ctctgcctcc
     1201 ggcaacagaa ccgatgtgac ttcattctgg atcgcgcgta tccattccga agtggactga
     1261 aaatcgtgcc gtgggaactt tcgtcgcaaa cggtcgcgat tttcagctga aagcagcctg
     1321 aagcaggact caaagttctc cggtttgttt gctgaagatt ctagaatctc gcaaaaggag
     1381 cgaagcatgt gagcttcgcg ataagccaca tgatcaggat aagtgctaca tctttccatg
     1441 tcgttctcga ggaaaagctg cattcgacgt ttccgccaca tgctttttgc aatggtaaag
     1501 actgcatctt cctcggttgg accaacgggg ttccattccg caatgaccgc ggtatgcaat
     1561 atccgaaatt gttcaggatc ctcccaagga aggattacta ttttcgcgaa cactccatgc
     1621 ttcagagcat taccgtgctt tccggctcgg tgacttgact tatcttcagg gcctctcagg
     1681 ccggacgcca attcctccga gttcaagcga acacgcttaa ccatcagcat tctcctattt
     1741 ggcgcctgcc taccctgcct tggttttggc aggacggtcg cgtcatgcct ttaaccgaaa
     1801 tctcgagggt tttgggtagc aaagctgctt atctgtattg acgcctggcg cgcgctccca
     1861 aatctgttgc ggatcttctc cagaacgtga ttcgctctta gcctacattc ggactacacg
     1921 acagaaaagg cggcgtttgc gtcagcaccg tcagcatcgt ctgcagctgc gagccagccc
     1981 tgcccaatcg agcacgggtc gaccaaatcg gagcgataat tggtgtcgcc gatctcatcg
     2041 gaagcttgat gtcgggggct gctgacggtg ctgacggtat gttcgctact cctgctcatg
     2101 cggatcattc tcgttcctcc tcggcccttc ctagagaatg taacctcgat gcccagggtc
     2161 cgcaggaaca tctgcgcccg ccgcagtcgg ccggcgagcg ctcgcggcgt ttttggccaa
     2221 gcggcgcctc tcttccaaag ctcgtcactg gcaaaatcgg ctgcacgtaa aagatctgcg
     2281 ggcaagcgtc cgccggccga tgtgggcacg cgttcactcc gattaaccga catgaaggcc
     2341 ggcatttgca tccgcaccgt cagcatcgtc agcggttgtg acacagaccg gccccatcgg
     2401 gtaccgaccg actaaatctg agcgagggtc gtcgtcgcaa acgtcaccgg cccgtcgcgg
     2461 tggaggctgg tcgcagacgc tactgacggt agtgacgata ttttcctgag ttgcatgtat
     2521 cctaatgatc ctgcttccgg cccggccttc ccggctgaag gcaatgtcga tgcccagtgc
     2581 ccgcaggaac gtctgcgcgc gacgcaggcg gccagcaagc gtgcggggat ttttgggcca
     2641 gccagtgcac tcattcaaaa ggtcgtggtt gctgcggtgg gcgccggccc gccaaagctc
     2701 ggcggcactg cccgtccatg agccgcgctc ggccatgatg tcccgcacgc aagcggcgac
     2761 cgggtcggca tcgattatgc cctcgaccgc ggtcctgcgg tttgctgcgt aggcccgagc
     2821 gaaagtgccg gggggccaca gggctgtttc gcaagccgcg gcccagaggg cgaaatcggc
     2881 catgcgtggt agccggtcga ggtgaattcg acccagggcg cggaggccgt gcaccaccgc
     2941 gtcgagcagt gcgcccagca tgcgggggcg tacgatttcg aactcgcgcc acagctcgtt
     3001 ctcgggacgc cgttgcgcct cgccaatggg tgccaaggtc agaaaaatcg cccggtcgcc
     3061 gagatccggc cggctgatca aatcttcgat accattcaag aggatcgggc gtgccgcttc
     3121 gaaaagcacc tcctcgtcgt cggtatagag ctgccggacc gcgaagctgc cgccgctcgc
     3181 gagccggcaa agcgcgtcgg agagccaaac aggcaagccg gagagattat cgaatgcgag
     3241 caggtagccg ttattggctg taatcatcag ctcgcgttcc tcgcgcgaga gcgagcgcac
     3301 gggggcggta ttggagtcta tcagagcctt gagcagctta gacaagacgg tcttggccga
     3361 gccctgctcg cccgaaatcg ccaagagcgg gtaggggcca ttcggtcgaa gtgctgccaa
     3421 cagccaggcg acgatcaata cgaagtcatc tcggctggca agattgagga aggaatggag
     3481 gggttcgatc gacccgccct gctgaggcac aggcagcggc aacatgccgg cgggccggcg
     3541 gaagcggacc ggcgggcatg caatcatgcg ccacccatcc ggcccgatgt cgacagcgcg
     3601 ccactgctca tcagcgagat cgagatagat gtggcccgca tgctcggcga tgcggacgtg
     3661 gacggcgcgc tcgggaccgt cgaattgagc ccgcgcctcg agtagatcaa gcgctgagcg
     3721 aatctccgcc gcgctcgcgg cgcccccggt cgcctggtaa tagcagcgcc gcggccagcc
     3781 ccgaaaccgc ttactgcgga tcggccaggt ttcccggtga ccgtcgagcg tgatatcggc
     3841 aaatgcggtg ccggctgcgg cgtgaaagag gtcatcacac ggcgcgatgt cgatgagcat
     3901 atcgggcggt cgcttctagg ccggcgctta gctcttcacg gcctgagcgt ccgttttcg
//
LOCUS       DATLJT010000540         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_720303,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000540 DATLJT010000000
VERSION     DATLJT010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720303"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VM910_12520"
     CDS             <1..314
                     /locus_tag="VM910_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63390.1"
                     /translation="PNAGQGACQAFEDAYILARWLEACPDPVEAFTNFRRIRIPRVHG
                     VQRLSLSNARFKHMRDSTAQKESIATGKGSVHGNSDWVWGYDPIGGWDKEPLVPTAYA
                     A"
     gene            complement(334..1302)
                     /locus_tag="VM910_12525"
     CDS             complement(334..1302)
                     /locus_tag="VM910_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63391.1"
                     /translation="MKQKLLTGLVVFIAHLLAFQSAHAYPDRPIKLVVPSPAGGPPDQ
                     IARLISDKMAAALGQPVIVENRTGGAGGIIGAKSVAAAEPDGHTLFMGSTSSVLIAPL
                     IYKNAGYTAATFAPVARVAESSEMLALHPSVPANSVSELVGLAKSRPGALSYGSAGVG
                     TLPHLEGEFLKTAARIDMLHVPYRGGGQALTGLLGGEVQVLFSTLVQILPHVRDGRLK
                     ALAVTGERRSPLAADVPTMVESGFDQLITTSVNVIVAPPGTPLAIRRQINQAAAAALA
                     STEVQQAFARLGYEARPASPEDVASYLAEAQQRWSRLIETARISID"
     gene            complement(1371..2138)
                     /locus_tag="VM910_12530"
     CDS             complement(1371..2138)
                     /locus_tag="VM910_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVI63392.1"
                     /translation="MPKPMGLIFAAAAMLASPVFVSPVLAATCPAPLAQASRLVLVTT
                     QFMDTPLATLQLFRRASADMPWKRVSAAEPAVVGKAGLGWGYPFLDVKEGEEPEKVEG
                     DNRTPAGFFRIGASFGFAPSRRPGYIELKSGETVCVEDPSSPFYNTITQRANIGAVEA
                     DDMRSSALYRSGLFVDYPSDRATRRGSCIFIHIWSAPDTGTSGCVGLPEARVEALQEF
                     TRAGAVLAVLPATAFDRFQSCLPAPAARPATLTGGTR"
BASE COUNT          431 a          743 c          724 g          389 t
ORIGIN      
        1 tgcccaatgc cgggcaaggc gcctgccagg ctttcgagga cgcctacatc ctcgcgcgct
       61 ggctcgaggc ttgccccgat ccggtcgagg cctttaccaa cttccgccgc atccgcatcc
      121 cgcgcgtgca tggggtgcag cggctctcct tgtctaatgc tcgcttcaag cacatgcgcg
      181 acagcacggc gcagaaagag tcgatcgcca ccggcaaagg cagcgtccac ggcaactccg
      241 attgggtctg gggttacgat cccattggcg gatgggacaa ggagccactt gttcccaccg
      301 cctatgctgc gtgaggctgg atcgattgca gcgtcaatca atcgagatcc gagccgtctc
      361 gatcagacgc gaccagcgtt gctgcgcctc tgccaagtag gacgcaacgt cctcgggcga
      421 ggctggtcgc gcttcgtagc ccagtctcgc aaaggcttgc tggacctcgg ttgaggcgag
      481 cgccgcggca gctgcctggt tgatctgccg cctgatcgcc aatggtgtcc caggcggcgc
      541 gacgatcaca ttgaccgagg ttgtgatcaa ctgatcgaat ccgctctcca ccatggtggg
      601 aacatccgcc gccagcgggc tgcgcctttc gccggtcacc gccaaggctt tgagccggcc
      661 gtcgcgaaca tgcggaagga tttgcaccag cgtggaaaag aggacttgaa cctcgccgcc
      721 caggaggccg gtcagtgcct gaccgccgcc gcgataggga acatggagca tatcgatccg
      781 ggcggccgtt ttcaggaatt cgccctcgag atgtggaagc gtcccgacgc cggctgaccc
      841 atagctcagc gcacccggtc gcgacttcgc cagtccgacc aattccgaca ccgagttggc
      901 cggcacggaa ggatgcaggg cgagcatttc gctgctctct gcaacacgag cgacgggcgc
      961 aaatgttgcg gcggtgtaac cggcattctt gtagatcaaa ggcgcgatga gaacggaact
     1021 cgtgctcccc atgaaaagcg tgtggccatc cggctctgcc gctgcgaccg atttcgcgcc
     1081 gatgatcccg ccggcgccgc ctgttcggtt ttccacgatc accggctgcc cgagagcggc
     1141 ggccatcttg tcggagatga gccgcgcaat ctggtctgga gggccccccg caggcgacgg
     1201 cacgacaagt ttgatgggcc gatccgggta agcatgcgcg ctttgaaatg ccagcaggtg
     1261 cgcgataaac acgaccaagc cggtgagcag tttctgcttc atggtccctc ccgaaggacg
     1321 tacggacgac agccgtgtcc gagcgctgcg gccgcggtga taaagtccgc ttaccgagtc
     1381 ccgccggtga gcgtcgcggg gcgggcggcc ggcgccggca ggcagctttg gaaacgatcg
     1441 aacgccgtcg ccggcaggac ggcgagcacc gcaccggcgc gagtaaattc ctgcaaggct
     1501 tcgacccggg cttccggaag ccccacacag cccgacgttc ccgtgtcggg cgcactccag
     1561 atatgaatga agatgcacga gccgcgtcgc gtggcgcgat cgctcggata gtccacgaag
     1621 agaccgctgc ggtagagcgc actgctgcgc atgtcatcgg cctcgaccgc accgatgttc
     1681 gcgcgctggg tgatggtatt gtaaaaaggc gaggagggat cctccacgca gaccgtctcc
     1741 cccgacttga gctcgatgta gccaggccgc cgggaaggcg caaaaccgaa gctcgccccg
     1801 atacgaaaga aacctgccgg tgtccggttg tcgccctcaa ccttctccgg ttcctcaccc
     1861 tccttgacgt cgaggaacgg gtagccccag ccaagcccgg ctttgccgac gacggccggc
     1921 tcggccgcgc tgacgcgttt ccaaggcatg tcggccgagg cgcgcctgaa gagctgcaac
     1981 gttgcaagcg gcgtatccat gaattgcgtc gtgaccaata cgagccgcga tgcctgcgcg
     2041 agaggtgcag gacaagtcgc cgccaagaca ggtgaaacga agacgggcga agcaagcatt
     2101 gccgccgcgg cgaatatcag gcccatgggt ttaggcatcg ctcgcccgcc catcgttcgc
     2161 gtgcccatcg ttcgcgtgcc catcgttcgc gtgcccacgc gttcgcaaac cagtttgcaa
     2221 gtcgccaaaa ctgtaaaggc gaggctatca tccgcgcgcc ggtcacgcga gccgcaagct
     2281 tgcaggt
//
LOCUS       DATLJT010000541         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1410089,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000541 DATLJT010000000
VERSION     DATLJT010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410089"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VM910_12535"
     CDS             <1..349
                     /locus_tag="VM910_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HVI63393.1"
                     /translation="LPAWLVPLAGRVERFAALPSGSIKHALCTEYDVGAGIGWHRDKK
                     HFERVFGLSLASACKFRFRRKIGNNWERFMLNAEPRSLYVMTGASRHVWAHSIPPVET
                     PRYSITFRTMAQA"
     gene            446..1429
                     /locus_tag="VM910_12540"
     CDS             446..1429
                     /locus_tag="VM910_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63394.1"
                     /translation="MKRREFIALLGGAAAWPLAARAQQPAMPVIGYIDAGFPEPAAHL
                     VAAFLRGLGETGFAEGRNVAIEFRWAHNNNERLPELAADLVRRRVAVIVAPQSTAAVM
                     EAKAATRMIPIVFGTGGDPVQAGLVTSLNRPGGNITGITFMNVAITAKRLGLLHELLP
                     NATRFAVLINPKNSIIAETMVTDAKAAASAMGRQVEIVKASTNREIDSAFSVLKEKRV
                     DALLVAPDTLMANRRVQIAMLAVRDAIPLMAVQRELAEAGGLMSYGTNFMETYRQTGI
                     YTGRILKGENPADLPVLRATKFEFVINLQTARTIGLEIPPTLLARADEVIE"
BASE COUNT          315 a          455 c          468 g          298 t
ORIGIN      
        1 gctgccggcc tggctggtgc cgctggccgg acgggttgaa cgcttcgccg ccttgccttc
       61 gggcagcatc aagcatgcgc tctgcacgga gtatgatgtc ggcgccggca tcggctggca
      121 ccgcgacaaa aagcacttcg agcgggtgtt cgggctctcc ctcgcctcgg cctgcaaatt
      181 ccgtttccgc cgcaagatcg gcaacaactg ggaacgtttc atgctcaacg cggaaccacg
      241 ctcgctctac gtgatgaccg gcgcctcccg ccatgtctgg gcgcacagca tcccgccggt
      301 cgagaccccg cgctattcga tcaccttccg tacgatggcc caagcctgag catcggattc
      361 gacttgaaat cccgcagcgc agcagtctcc tgcggtagct gaggtgtgct atcctttctg
      421 tcggaagcac gggaggtatc gggcagtgaa acggcgcgag ttcatcgcgc tgctcggcgg
      481 cgcggccgcc tggccgctcg cggcgcgggc gcagcagccg gcgatgccgg tgatcggata
      541 tatcgacgct ggtttccccg agccggctgc acatctcgta gcagcgttcc tcagaggttt
      601 gggcgaaacc ggattcgcgg aaggtcgcaa tgtcgcgatc gaattccgct gggcgcacaa
      661 caacaatgag cggctgccgg aattggcggc cgatctggtt cgtcggcgcg tggcggtgat
      721 tgtcgcacca cagagcacgg ctgcggtaat ggaggccaag gctgcgacca ggatgatccc
      781 gatcgttttc gggactggag gcgatccagt ccaagctggt ctcgtcacca gcctgaatcg
      841 accgggcggc aacattaccg gtatcacatt tatgaacgta gctattaccg cgaagcggct
      901 cggtctgtta cacgagctac tcccgaatgc cacacgcttc gccgtactta ttaatccaaa
      961 gaattcgata atcgccgaga caatggtcac ggacgcgaag gcggcagcat cggccatggg
     1021 gcggcaggtc gaaattgtca aagcgagcac taatcgcgaa atcgattcag cattttcggt
     1081 cctcaaggag aagcgcgtag acgcgctgct cgtcgctcct gacacgctaa tggcgaatcg
     1141 tcgggtgcaa atcgccatgc tggcggtgcg cgacgccata ccgctgatgg cggtacaacg
     1201 agaattagcg gaagcgggtg gattaatgag ctacggaacg aactttatgg aaacctatcg
     1261 ccagaccggc atctacaccg gccgcatcct caaaggcgaa aaccccgccg acctgccagt
     1321 gctaagggcg accaagttcg agttcgtcat taacctacaa acggcaagga cgattggtct
     1381 tgaaatcccg ccaaccttgc tcgcccgcgc cgacgaggtg atcgaatgag caactgtgtt
     1441 gttcgttcag gcggattgcg gctgccatgc agcattatcg cgaagcatag cgttgagggt
     1501 tgtgatcatt tttcgcatga cggcgacgat gatgac
//
LOCUS       DATLJT010000542         3580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2329300,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000542 DATLJT010000000
VERSION     DATLJT010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329300"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VM910_12545"
     CDS             complement(<1..489)
                     /locus_tag="VM910_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI63395.1"
                     /translation="MTAITEFTPRPRYRFLLDRAELLGPLFIAPAILYVLVLVALPFF
                     LAIYYSISAFTIFDPSYRFVGAKNFFDVIQSEVFQRTLINTFIFTIGAQIIGLLLGKF
                     AAMLLMQDFPGRGLARALVVLPWAVPVSLATLAWQWMFDSLYSVINWTLIAIGLVNAE
                     HPP"
     gene            complement(505..1527)
                     /locus_tag="VM910_12550"
     CDS             complement(505..1527)
                     /locus_tag="VM910_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63396.1"
                     /translation="MASVSTIDIRKQFGEVTAVDGVSLEARDGEFMVLLGPSGCGKTT
                     LLRIIAGLEKATSGEVRIGGHVVNDLPPRARGVAMVFQSYGLYPHLTVWNNIAFPLRT
                     QGTSREQISRKVEWASRLLGIDHLAHRRPRQLSGGERQRVALARALVREPTVFLLDEP
                     LSNLDAKLRASARSELKGFQRTVRTATIYVTHDQVEAMGMGDRIAVMDHGRVRQVGSP
                     IEIYEYPADEFVASFLGTPPMNLIERDGGSLGFRPEHFLPKELLNGFEVQEFQFRVDR
                     MEYLGSERILYGALDGRAAKREVTAKLPAHLALDGISAGAWHPFAVRERELRYFDREG
                     RQTLNR"
     gene            complement(1744..3096)
                     /locus_tag="VM910_12555"
     CDS             complement(1744..3096)
                     /locus_tag="VM910_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI63397.1"
                     /translation="MGYGGNKQGSVTRRHFLKTTSAIAGAAGLAPALSAPLVSTVLAA
                     TKTLRILQWSHFIPEYDRWIDGFAKEWGKQNGVTVTVDHIPHLELPARAAAEVSAGAG
                     HDIFHFNGSGGPHLYAKHTLDLTALIQEVEKKHGKVLPMGRQIAWNEETKGWSAFPDY
                     FIDFPGLYRKDLWDEIGTPPDKWEDVRVGGAKLKQKGFPVGIALSHCVDANNSHSSVL
                     WSHGASVCDETGKRVTIDSKETLEAVKYVRALYKEAMDPEVLSWDDASNNRFLASGRG
                     SWIHNPISAYRSIQKANPELADKIFMTKTPAGTVRRLAAGAPNSYVIWKFARNQDTAI
                     EFLKYYADHYTDAFKASTGYNHPLFENLVPKPMPILSNDPSSHPPDKMKVLETADEWC
                     VVFGYPGPGTPAADEVANNYIIPDMMANAATDKMSPEDAVKWAANEIKGIYDKWATRT
                     "
BASE COUNT          616 a         1108 c         1169 g          687 t
ORIGIN      
        1 cggcggatgc tcggcgttca ccaggccgat cgcgatcaga gtccagttga tcacgctgta
       61 gagcgagtcg aacatccatt gccaggcgag cgtggcgagc gacaccggaa ccgcccacgg
      121 cagcaccacg agcgcgcggg caagaccgcg gccggggaaa tcctgcatca acagcatagc
      181 ggcgaacttg ccgagcagca ggccgatgat ctgggcgccg atggtgaata tgaaagtgtt
      241 gattagcgtg cgctggaaca cttcgctctg gatcacgtcg aaaaagttct tcgctccgac
      301 gaaccgatag ctcgggtcga agatggtgaa ggcgctgatg ctgtagtaga tcgcgaggaa
      361 gaacggcagc gcgaccagaa cgaggacata gagaatggcc ggtgctatga aaagcgggcc
      421 gagcagctcg gcgcgatcga gcaggaaccg gtatctcggc cggggagtga attccgtgat
      481 cgccgtcatg gcgaagactc tccgttatcg gttgagggtc tggcgcccct cgcgatcgaa
      541 gtagcgcagc tccctttccc gcaccgcgaa cgggtgccac gcaccggcgc tgatgccgtc
      601 aagcgcgagg tgcgccggca acttggcggt cacctcgcgc ttcgccgccc gcccgtcgag
      661 ggcgccgtag agaatgcgct cggagccgag atactccatg cggtcgacgc ggaattggaa
      721 ctcctggacc tcgaaaccgt tgagcagctc cttcgggagg aagtgctccg ggcggaagcc
      781 gagcgagccg ccgtcgcgct cgatcaggtt catcggcggg gtgccgagaa agctagcgac
      841 gaattcgtcc gccgggtact cgtagatttc gatcgggctg ccgacttgcc gcactctgcc
      901 gtggtccatc accgcgatgc gatcgcccat gcccatagcc tcgacctggt cgtgggtcac
      961 gtagatggtc gccgtacgca cggtccgctg gaatcctttg agttcgctgc gcgcgctcgc
     1021 gcgcagcttg gcgtcgaggt tcgacagcgg ctcgtcgagc aggaacaccg tcggctcgcg
     1081 gacgagcgca cgcgccagcg ccacgcgctg gcgctcgccc cccgagagct gccgcgggcg
     1141 ccgatgcgcg agatggtcga tgccgagcag ccgtgaagcc cactcgacct tgcggctgat
     1201 ctgctcgcgc gacgtgcctt gcgtgcgcaa tgggaaggcg atgttgttcc aaacggtcaa
     1261 gtggggatag agaccgtagc tctgaaacac catggcgacg ccgcgagcgc gcggcggcaa
     1321 gtcgttgacc acgtggccgc cgatccgaac ctcgccggac gtggctttct ccagcccagc
     1381 aatgatgcga agaagcgtgg tcttgccgca gcccgaagga ccgagcagca ccatgaactc
     1441 gccgtcgcgc gcctccaggc tgacgccgtc gacggcggtg acctcgccga actgttttct
     1501 gatgtcgatg gttgaaaccg aagccaactc gacctccgag ggcacgtggg gaaaacggaa
     1561 gcggctcgcc gtccgatcgc ccagagactg ggcttccgcg gccggcggcg ggtgaccgaa
     1621 tcggacgcct gccgccgacg cttaagcaag cctcgagcgg gatgacttca tcttcgaatc
     1681 gtcatcccac tctggttttc cctggagcat gttcgaccgg cattcactct tcgcgatcat
     1741 gctctaggtg cgtgtcgccc acttgtcgta gatgcccttg atctcgttgg ccgcccattt
     1801 gacggcgtct tccggcgaca tcttgtcggt cgccgcattc gccatcatgt cggggatgat
     1861 gtagttgttc gccacctcgt cggcggccgg agtgccgggt cccggatagc cgaacaccac
     1921 gcaccactcg tcggcggtct ccagcacttt cattttgtcc ggcggatgcg acgacgggtc
     1981 gttcgacagg atcggcatcg gcttcggcac caggttttcg aacagcggat ggttgtatcc
     2041 ggtggaggcc ttgaaggcgt cggtgtagtg atcggcgtag tatttgagga attcgatcgc
     2101 cgtatcctgg ttgcgcgcga acttccagat gacatacgag ttcggcgccc cggccgccag
     2161 acggcggacg gttccggcgg gcgttttcgt catgaagatc ttgtcggcca gctctggatt
     2221 ggctttctgg atcgatcggt acgccgagat cggattgtgg atccacgagc cgcggccgga
     2281 ggccaggaat cggttgttgc tggcgtcgtc ccacgacagc acctcgggat ccatcgcttc
     2341 cttgtagagt gcgcggacgt acttgacggc ctcgagcgtc tccttggagt cgatcgtgac
     2401 gcgcttgccc gtctcgtcgc ataccgaggc accgtggctc cacagcacgc tgctgtggga
     2461 gttgttggcg tcgacgcagt gggagagtgc gatgccaacc ggaaagccct tctgcttgag
     2521 ctttgccccg ccgacgcgaa catcctccca cttatccggg ggtgtgccga tctcgtccca
     2581 cagatccttg cgatagaggc cggggaaatc gatgaagtag tcggggaagg cgctccagcc
     2641 cttggtttcc tcgttccagg cgatctggcg gcccatcggc agcaccttgc cgtgcttctt
     2701 ctcgacctcc tggatcagcg cagtgaggtc gagcgtgtgc ttggcgtaga gatgcggtcc
     2761 gcccgatccg ttgaagtgga agatgtcgtg cccggcgccg gcggagacct cggcggcggc
     2821 gcgcgccggt agttcgaggt gcgggatgtg gtcgacggtc acggtgacgc cgttctgctt
     2881 accccattcc ttcgcgaagc cgtcgatcca cctgtcgtat tcggggatga agtggctcca
     2941 ctgaaggatc ctcagcgttt tggtcgcagc gaggacggtc gacacgagcg gcgccgagag
     3001 tgccggcgcc aagccggccg cccctgcaat ggcgctcgtc gtcttcagga agtgccgtcg
     3061 cgtgacggaa ccttgcttgt ttcctccata tcccatcgca caactcctgt ttgctatggt
     3121 gacgatgatg ttgctgatgc tcactccctg attgtgctcg acaatcggtt ccgcggcgcg
     3181 ccatcggcct gacggacgtg gcgcttcacg cggtcccgtc ccggtcgaat ggcccgagcg
     3241 cgctcaggcg caaatgtgca acgcagcatg aatgccgggt tcagtacgac gcacccgccg
     3301 gagcgggcca acgcccggcc ctccgctttt ttgcgctttg ccgcgcgctt gcggctccgg
     3361 cttggaagcc gcaacacggg gtgtcgccca taccgctagc gcaatatgtc catgcgccgt
     3421 tggtatggca agcggatttc acctcgatgc gatgatcaga cgcgccaacc gaattgtgtc
     3481 aaagcgtcag ggtgggcgcc aagcggcagg tccgtccgtc tctggttcgt ctccaaagcg
     3541 acttgaactc gatggaattg ccgtcgcgaa gcgccagagc
//
LOCUS       DATLJT010000543         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2099274,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000543 DATLJT010000000
VERSION     DATLJT010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099274"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VM910_12560"
     CDS             <1..146
                     /locus_tag="VM910_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI63398.1"
                     /translation="IQSDLVRARGEVFVSRAKSTSEYLGKLVANGAKTAQDNFSKVYN
                     KTV"
     gene            341..1627
                     /locus_tag="VM910_12565"
     CDS             341..1627
                     /locus_tag="VM910_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HVI63399.1"
                     /translation="MAVQGTFSFDMPGDAQRAADLKRVKTQATLVLVGSLALFAIAKV
                     LLHVHPAFGFVAAFAEAATIGGLADWYAVVALFKRPLGLPIPHTAIIQSNQHRIADKL
                     GEFIEVHFLEQGPVEAKLRQIDFGSFVADWLRDRKRSGDLARFALRLLPDAVAATETS
                     GLMTYITRRMTAQLQAIDLAPLAAGTLRTFVKEGRHQGLLDDLLRVVHDTLTTPETMA
                     MIRVKIREELPTLLRLYRADRFLVNKIVASASHFFEEVRRDPKHPFRGEFDRMVLSFV
                     DRIGTDKAYAVRIDGLKRDLLARPELSELARTVWSNTRSFIERSASGETQVLQNHLTG
                     MLVAAGEALAQDAELRGEINQGLVAVLRSFIADQKSGVSTFISDQVKSWNMAQLISLI
                     EINIGRDLQYIRFNGSLIGGLAGLGLYTAEYLLRLL"
     gene            1814..2584
                     /locus_tag="VM910_12570"
     CDS             1814..2584
                     /locus_tag="VM910_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI63400.1"
                     /translation="MSVAAQTLRAPSRTLMFLEGRALHELGAFVGALPLLSLAPRGDG
                     HPVLVLPGLIASDVSTRPLRAFLRNRDYAVTGWQQGRNLGLRPGVQSAMTELVEKLND
                     LHGRKVSLVGWSLGGLYARQLAKLMPERVRSVITLGSPFAGSPKSTNAWRFYEMASGR
                     RADEEDPRFGGSLSVPPPVPTTAIFSRTDGICAWQGCKERTSAKSESIEVESSHCGMG
                     HHPAVVYAVADRLAQPEGQWKPFDRSGWRALLYPDPNR"
     gene            2710..>2801
                     /locus_tag="VM910_12575"
     CDS             2710..>2801
                     /locus_tag="VM910_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HVI63401.1"
                     /translation="MPQPLDRIIEELKREPSRTGSIVITVFGDA"
BASE COUNT          490 a          870 c          880 g          561 t
ORIGIN      
        1 agatccagtc cgatctcgtc cgcgcgcgtg gtgaagtttt cgtctcgcgg gccaagtcga
       61 ccagcgaata tctcggcaag ctcgttgcca acggtgcgaa gactgcccag gacaacttct
      121 cgaaggtcta taacaagacc gtctgattgg ccttttgtag attgagttga gtagttaagg
      181 cccgcgtcaa gcgggccttc tttttggtac gaacttcaat acatcccctc atggcgagga
      241 gcgcgcgtcg cgcctcgaac catgaggccc aagtgtggcc tcatccttgg agacgccgcc
      301 aagaggccgc tcctcaggat gctggtcgac gggagttgcc atggccgtgc aaggcacctt
      361 ctcctttgat atgcccggtg acgcccagcg cgccgccgac ctcaagcgcg tcaagacaca
      421 ggcgacgctc gtgctggtgg gttcgcttgc gctgtttgcg atcgcaaagg ttttgctcca
      481 cgtgcatccc gcctttggct tcgtcgcggc ctttgcggag gccgccacca tcggcggcct
      541 cgccgactgg tacgcggtgg tggcgctgtt caagcgaccg ctgggattgc cgattccgca
      601 tacggcgatc atccagagca accagcatcg catcgccgac aagctcggcg agttcatcga
      661 ggtgcatttc ctcgaacagg ggccggtgga ggcgaagctt cgccagatcg atttcggctc
      721 gttcgtcgcc gactggttgc gcgatcgcaa gcgctccggc gacctggcgc gctttgcgct
      781 gcggctgttg ccggacgcgg tcgcggcaac cgagacctcc gggctgatga cctacatcac
      841 gcgccggatg accgcgcagt tgcaggcgat cgatctcgcc ccgcttgccg ccgggacgct
      901 gcgcaccttc gtcaaggagg gacggcatca gggactgctc gacgatttgc tgcgcgtcgt
      961 ccacgacacg ctgacgacgc cggagacgat ggcgatgatc cgcgtgaaaa tccgcgagga
     1021 actgccgacg ctgctgagac tctatcgcgc cgacagattc ctggtgaaca agatcgtggc
     1081 gtcggcgagc catttcttcg aggaggtacg gcgcgatccc aagcatccgt tccgcggcga
     1141 gttcgaccgc atggtgctgt cctttgtcga ccgcatcggg accgacaagg cctatgccgt
     1201 gcgcatcgac ggcctgaagc gcgatctttt ggcgcgtccg gagctgtcgg aacttgcgcg
     1261 taccgtgtgg tcgaacacac gcagcttcat cgagcgcagc gcgtccggcg agacacaggt
     1321 gctgcagaac catctgaccg ggatgctcgt cgccgccggc gaggcgctgg cgcaggatgc
     1381 ggagctgcgg ggcgagatca atcagggtct ggtagcggtg ctcagaagct tcatcgccga
     1441 tcagaagagc ggcgtttcga ctttcatctc cgaccaggtc aagtcctgga atatggcgca
     1501 gttgatttcg ctgatcgaaa tcaacatcgg acgcgacctg caatacatcc gcttcaacgg
     1561 ctccttgatc ggcggcctgg cggggcttgg gctctacacc gcggaatacc tgctgcggct
     1621 attgtgacca attgatcacg ccacgcattt tccctgtggc aagtgtgagc cgaaccgctt
     1681 gaaacgccca aaactgttcc ttagcttttc atttggagtt gttgcactgc gaaacgattc
     1741 cacgaggctt gcgttgtccc gttgatccgt tgaacggctg ccgcagcagg cggcggccac
     1801 aaaaggagcc gagatgtccg ttgctgcgca aacgcttcgc gcgccgtcca gaacgctgat
     1861 gtttctggaa ggccgtgccc ttcacgaact tggcgccttc gtcggcgcat tgccgctgct
     1921 cagcctcgcg ccgcgcggcg acggccatcc cgtgctggtg ctgccgggac tgattgcgtc
     1981 cgacgtttcc acgcggccgc tgcgcgcctt ccttcgcaat cgcgattatg cggtcaccgg
     2041 ctggcagcag ggccgcaatc tcgggcttcg cccgggcgtg cagagcgcga tgaccgaact
     2101 cgtggaaaaa ctgaacgacc tgcatggccg caaggtctcg ctggtcggct ggagcctcgg
     2161 cggtctttat gcgcggcagc tcgccaagct gatgccggag cgcgtgcgca gcgtgatcac
     2221 gctgggaagt ccgttcgcgg ggtcgccgaa atcgaccaac gcctggcgct tctatgagat
     2281 ggcgagcggc cgtcgcgccg atgaagagga tccgcgcttt ggcggatcgc tctccgtgcc
     2341 gccgccggtg ccgaccactg cgatcttcag ccgcaccgac ggcatctgcg cctggcaggg
     2401 ctgcaaggaa aggacctcgg cgaagtctga gagcatcgaa gtcgaaagca gccattgcgg
     2461 gatgggtcat catcctgccg ttgtctatgc ggtcgccgat cgcctcgccc aaccggaagg
     2521 gcagtggaag ccgttcgacc gctccggctg gcgcgcgctg ctctaccctg atccgaaccg
     2581 gtaggcgttt tgggagcggg caaatcggtt actgcattcg actctattat cgcctggtgt
     2641 tcttcggttc taacattcgc ataagtgctc gccgcgatgg catgcggatg ttagaagcgg
     2701 gacatcagca tgccgcagcc gcttgatcga atcattgaag agctcaagcg cgaaccttcg
     2761 cgcaccggct ccatcgtcat caccgttttc ggcgatgcca t
//
LOCUS       DATLJT010000544         4051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_491445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000544 DATLJT010000000
VERSION     DATLJT010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VM910_12580"
     CDS             <1..324
                     /locus_tag="VM910_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63402.1"
                     /translation="DPQFKTDPGVIPSDEECEKLGITVVKTTFQHNDMRDTGSEEAKA
                     SIQGMLDKFLADTDTEAAPVEANSPKVLEPGPVAPYVPAGKEPRKETERETGKEAKSA
                     SAETH"
     gene            377..1132
                     /locus_tag="VM910_12585"
     CDS             377..1132
                     /locus_tag="VM910_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI63403.1"
                     /translation="MSGEPTKSKSGDVASRDGAKADSLPDAKASTSDDIAAFVAKARA
                     MSPHEAGARGRLVFALDATMSRQPTWDMACTLQADMFREAASLGSLDIRLVYYRGFNE
                     CRATGWISDSAELARLMSKIDCRGGNTQIGKVLSEARREAAASGVRAVVFVGDAMEEK
                     VDDLCAKAGELGMLKVPVFVFQEGHDAVAERAFREIARLTGGAWCRFDPGAAAQLREL
                     LRAAAAYAAGGRDALKRLSARESGAAKLLGQMK"
     gene            1172..1903
                     /locus_tag="VM910_12590"
     CDS             1172..1903
                     /locus_tag="VM910_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HVI63404.1"
                     /translation="MPTLIAGAIAVLVLYLLLQMYRAANPAVLARALKIVGGVIALAV
                     AAFVGVRGELAVAIPIGIFGAGLLGWSPFGTAAFSNFGNIFSGAASRRSSGQSSQVRS
                     QYLDMTLDHDSGALTGRIVAGPHAGHSLDEFDLNGLIGMISGFDAESVALLESYLDRR
                     FPAWRQHAQGNTAGGQRRAPPGGKMTDEEAYQILGLQPGAGREEIGRAHRALMKKLHP
                     DQGGSTYLAARVNEAKDTLLRTHNG"
     gene            complement(2122..3990)
                     /locus_tag="VM910_12595"
     CDS             complement(2122..3990)
                     /locus_tag="VM910_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVI63405.1"
                     /translation="MLREHLASSRLVRVGVLGLVAFAAAAVTSESAQARRYSRHHSTH
                     HVRHHDSDDSYSPQFSSIIVDGNSGATLAATSPDGVRHPASLTKIMTLYLLFERLDAG
                     KMKLDTEMPVSEHASEQDPTKLGLRPGQTIKVEDAIKGLVTRSANDAAVVIAEAIGGS
                     EDEFARMMTRKAHALGMNRTIYRNASGLPNDEQVTSARDQAMLGRAIQDRFPRYYRYF
                     STQAFNFRGQGISNHNHLLGSVEGVDGIKTGYTRASGFNLVTSMHRGNRFLVGVVMGG
                     RSGGARDATMRSLLAANLEKAATTRTAAAITERSGSEATTDVADAQPKPAPAPVAQVQ
                     ATPVSPDTWTPPAMRNPPAKVTAALAQASAAMPPAQAKTEPAPLSSGVIQAQAMAAIP
                     GSFEPMKPVRVKTVQVKAGSTKVASAGPSQPSPPIISALPQAQPAPAETSNAVVAKAS
                     EVGKPEPVKAAEAVRAEAARPDLPPQPPGHGTGNGLLGVLPASSVVPASSAAQPVAVA
                     APQPAVAVATAQPASPPPPQISQQTTPSKPVVHTGWIVQVGALESEDEAQQRIEAAKN
                     NARGLLSKADSFTEPIISKDNRKLFRARFAGLERDQAEAVCRALRHSDIVCITVRN"
BASE COUNT          727 a         1229 c         1357 g          738 t
ORIGIN      
        1 gacccgcagt tcaagaccga ccccggcgtg attccgagcg acgaagagtg cgagaagttg
       61 ggtatcacgg tggtgaagac caccttccag cataacgaca tgcgcgatac cggatcggaa
      121 gaggcgaagg cctcgatcca gggcatgctc gacaaattcc tggccgatac cgatactgag
      181 gctgcgcccg tcgaagccaa ttcgcccaag gttctcgaac cgggcccggt cgcaccatac
      241 gtaccggccg ggaaggagcc ccggaaggaa accgagagag aaaccgggaa agaggccaag
      301 tccgcgagcg cggagacgca ttagggtcaa aagataaccc tggttatccg agggcttgca
      361 gcacacgcga gcgccgatgt ccggcgaacc gaccaaatca aaatcaggcg acgtggcttc
      421 gcgcgatggc gcgaaggccg actcattgcc ggatgccaag gcctcgacat cggacgacat
      481 cgccgccttc gttgcgaaag cgcgcgcgat gtctccgcat gaagcgggtg cgcgggggcg
      541 gctggtcttt gcgctcgacg ccaccatgag ccgccagccg acctgggaca tggcctgcac
      601 gctgcaggcc gatatgttcc gcgaggcagc ttcgctcgga agcctcgata ttcgcctggt
      661 ctactatcgc ggcttcaacg aatgccgtgc gacgggctgg atttccgaca gcgccgagct
      721 tgcaaggctg atgagcaaga tcgactgccg cggcggtaac acgcagatcg gcaaggtact
      781 gtccgaagcg cggcgcgagg cggctgcctc aggtgtgcgc gcggtggtgt tcgtcggcga
      841 cgccatggaa gagaaggtcg acgacctctg cgccaaggcc ggcgaactcg gcatgctcaa
      901 ggtgccggtc ttcgtgttcc aggaagggca tgatgccgtt gccgaacggg ccttccgtga
      961 gatcgcgcgg ctcaccggcg gtgcctggtg caggttcgat cccggtgcag ccgcacaatt
     1021 gcgtgaactg ctgcgcgccg cggccgccta cgcggcgggc ggccgcgatg ccctcaaacg
     1081 gctgtcggcg cgcgagagcg gcgccgccaa attactcggc cagatgaagt gatctctcac
     1141 cttttggcgc caagccgatt atattaaggc catgccgacc ctgatcgcgg gcgctatcgc
     1201 tgttctcgtc ctctatttgc tgctgcagat gtaccgcgcg gccaatccgg ccgtgctcgc
     1261 gcgtgcactc aagattgtcg gaggcgtgat cgcgctcgcc gtcgccgctt ttgtcggcgt
     1321 gaggggggag ctcgcggtcg caatcccgat tggcatcttc ggcgccggat tgcttggctg
     1381 gtcgccgttt ggcacggccg ccttcagcaa tttcggcaac atcttctcag gtgccgcctc
     1441 gcggcgctcc tccggccaga gctcgcaggt tcgctcccag tatctcgata tgacgctgga
     1501 ccatgacagc ggtgccttga ccgggcgaat cgtcgcggga cctcacgcgg gccattcgct
     1561 cgacgaattc gatctcaacg ggctgatcgg gatgatctcg ggcttcgatg ccgagagcgt
     1621 ggccttactt gaaagctatc tggaccgccg gtttcccgct tggcgtcagc acgcgcaggg
     1681 aaatacggca ggcgggcaac gccgcgcgcc gccgggcggc aaaatgacgg acgaggaggc
     1741 ctatcagatc cttggcctgc aaccgggcgc ggggcgcgag gaaatcggac gagcccaccg
     1801 agcgctcatg aagaaactgc atcccgacca gggggggtcg acgtacctcg ccgcccgagt
     1861 aaacgaggcc aaggatactt tgcttcgcac gcataacggc taactccggc aacgctcaca
     1921 aacgccacaa agtcgctttc caggccgaga ctctggcaga cgcctgccgc cgtccgccgt
     1981 tgctccggcg tgaccttcgc tttcaaaaag ttttaagccc aaattcttga cgagaggttt
     2041 gcaggattgc gtcttcgggc agctaaaaaa gaagcccgcg cttttgcggc gcgggcatct
     2101 gctcgttcaa gccgttgata atcagttacg aacggtgatg cagacgatgt cggagtggcg
     2161 cagcgcgcgg cagaccgctt cggcctgatc acgctcgagg cccgcaaagc gagcgcggaa
     2221 gagtttgcgg ttgtctttgg agatgatcgg ctccgtaaag gagtcggcct tgctgagcag
     2281 cccgcgggcg ttgtttttgg cggcctcgat gcgctgctgg gcttcgtctt cgctttcgag
     2341 cgcgccgacc tgcacgatcc aacccgtgtg gacgacgggc ttgctcgggg tggtttgctg
     2401 gctgatctgc ggcggaggag gcgaggcggg ttgcgccgtc gcaaccgcga ccgccggctg
     2461 gggtgcggcg acggcgaccg gctgcgcagc ggacgaggca ggaacaacgc tggatgcggg
     2521 cagcacgccg agcaagccat tgccggtacc gtgaccaggc ggctgcggcg gcaagtctgg
     2581 tcgggcggcc tcggctctga cagcttcggc ggccttcacc ggctcaggct tgccaacctc
     2641 gcttgccttc gcgacgacgg cgttcgatgt ctccgcggga gctggctgag cctgcggcaa
     2701 tgcgctgatg atgggaggcg agggttggga tgggccggca gaagcgacct tcgtcgatcc
     2761 cgctttgacc tgaactgtct tgaccctgac aggcttcatc ggctcgaacg agccggggat
     2821 ggccgccatt gcttgagcct ggatcacgcc ggaagaaagc ggcgcaggtt cggtcttggc
     2881 ttgagcgggg ggcatggcgg cactcgcctg cgcaagcgcg gccgtcactt tggccggtgg
     2941 attgcgcatc gcgggcggcg tccaggtatc gggcgaaacg ggcgtggcct gcacctgtgc
     3001 gaccggcgca ggagccggct tcggttgggc atcggcgaca tcggtggtgg cctccgagcc
     3061 gctgcgttcg gtgatcgcag cagccgtccg cgtcgttgca gccttctcca gatttgccgc
     3121 gagcaggctg cgcatggtgg catcgcgcgc gccgccgctg cggccaccca tcacgacacc
     3181 taccaggaag cggttgccgc ggtgcattga agttacgaga ttgaaaccgg aagcgcgggt
     3241 gtagccggtc ttgattccgt cgacaccctc gacgctgccg agcaggtgat tatggttgct
     3301 gatgccctgg ccgcgaaagt tgaaggcctg cgtcgagaaa tagcggtaat agcgcggaaa
     3361 gcggtcctga atggcgcgac ccaacatcgc ctgatcgcgc gcactcgtca cctgctcgtc
     3421 gttgggaagc cccgaggcgt tgcgataaat ggtgcggttc atgccgagcg catgcgcctt
     3481 gcgcgtcatc atcctggcga actcatcttc gctgccgccg atcgcttcgg cgatgacgac
     3541 cgcggcgtca ttggcggaac gcgtcaccag acccttgata gcgtcttcaa ccttgatggt
     3601 ttggccagga cgtagcccca gctttgtcgg atcctgttcg gaggcgtgtt cagataccgg
     3661 catttcggta tcgagcttca tcttgccggc gtcgagacgc tcgaacagca gataaagcgt
     3721 catgatcttt gtgagagagg cgggatgacg gacgccgtcg ggactcgtgg cggctagcgt
     3781 tgcgccggag ttgccatcca cgatgatcga agagaactgc ggactgtagc tgtcgtcgga
     3841 atcgtgatgg cggacgtgat gggtgctgtg gtgccgggag tagcgacgcg cctgggcgct
     3901 ttcgctcgtg acggccgccg ccgcgaatgc aaccagcccc aaaacgccaa ctcgcaccaa
     3961 gcgcgaggaa gccaagtgtt cacgaagcat gaaatccccg tcccagtttc tcacttgatc
     4021 accggttcgt gaggcaaaat tgtgcccaat c
//
LOCUS       DATLJT010000545         1506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1640653,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000545 DATLJT010000000
VERSION     DATLJT010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640653"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VM910_12600"
     CDS             <1..256
                     /locus_tag="VM910_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63406.1"
                     /translation="TEAGPAAMIAPVAPGIGGHITALGHEPYRYTTFYVDVEDVAAYL
                     KKAESLGGKTLVPPIEIPTGTFAWMQDLEGNTIGLWKSRS"
     gene            complement(418..>1506)
                     /locus_tag="VM910_12605"
     CDS             complement(418..>1506)
                     /locus_tag="VM910_12605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI63407.1"
                     /translation="LARVPQLNPKQIDDVILGCAMPEGEQGMNVARIAALRAGLPVET
                     SAMTVNRFCASGLQAIALAAERIRGGGAQVIVAGGTESMSMVPMGGNKVSPNPWLVDH
                     FPDTYINMGLGTENIARKFGIGREQADRFAVESHRKAVASLAANKFSDETVPVEVKIT
                     SLPNGNGAKGKSAAKPATEVFVFDRDEMPRPDTSMEVLAGLKPAFHAKGTVTAGNSSP
                     MSDGAAAVVVMSAEKATEMGLEPQARFVSYATAGCPPEEFGIGPVYAIPKALDLAGLS
                     LNDMAVVELNEAFAAQSLAVIQQANLDAGRVNPNGGAIALGHPLGCTGAKLTASLIRE
                     LERRKARYGMVTMCIGGGMGAAGIFERL"
BASE COUNT          266 a          519 c          416 g          305 t
ORIGIN      
        1 taccgaagcc gggcctgcgg cgatgatcgc tccggtagcg ccgggtatcg gcggccacat
       61 taccgcgcta ggtcacgagc cctaccgcta caccacattt tatgtggacg tcgaggacgt
      121 tgccgcctat ctgaagaagg cggaatcgct cggcggcaaa acgctcgttc cacccatcga
      181 aatccccacc ggcacgttcg cgtggatgca agacctcgaa ggcaacacca tcggcctgtg
      241 gaaatcccgg agctgatgcg ggccagttcg cgctttgttt gcgccggggg ctgcgtacgc
      301 tgccggcgcc ggctgggcct ttctcttcgc ctcgtgccac tccgggtcag aacggctctc
      361 cggcgttgat cctttcgaga tgtccagcct cagccagcgg cctctcgggt attatcgtta
      421 cagccgctcg aaaatccccg cggctcccat gccgccgccg atgcacatcg tcaccatgcc
      481 gtaacgcgcc ttgcgtcgct cgagttcgcg gatgagcgaa gcggtgagct tcgctcccgt
      541 gcacccgagg ggatgtccga gcgcgatcgc gccgccgttc ggattgacgc gacccgcgtc
      601 caaattcgct tgttgaatca cggccagcga ttgtgcggca aacgcttcgt tcagctccac
      661 aaccgccata tcgttcaatg aaagccctgc caggtccagc gccttcggaa tcgcatatac
      721 cggtccaatg ccgaattctt ccggagggca tccggcggtt gcgtaagaga caaatctcgc
      781 ttgaggttcg agtcccatct ccgtggcttt ctccgccgac atcaccacca ccgccgcggc
      841 gccgtcgctc atcggggaac tgttgcctgc cgttaccgtc cctttcgcgt gaaacgccgg
      901 cttcagtccc gctagcactt ccatcgaagt atcgggacgc ggcatctcat cgcggtcgaa
      961 aacgaacacc tctgttgcgg gcttcgccgc cgacttgcct ttggcgccat ttccgttcgg
     1021 aagggaagta atcttcacct ccactggcac ggtctcatcg ctaaatttgt tcgccgccag
     1081 cgacgcaact gccttcctgt gcgactcgac cgcaaagcgg tcggcctgct cgcgcccgat
     1141 cccgaatttt ctggcaatgt tctccgtacc caggcccata ttgatgtatg tgtcgggaaa
     1201 gtgatccacg agccatgggt tcggcgaaac cttgtttccg cccatcggca ccatcgacat
     1261 cgactccgtg ccacccgcga cgatcacttg cgcgccacca ccacggatcc gttccgctgc
     1321 aagcgcaatg gcttgcagac cgcttgcgca aaaacggttc acggtcatcg ccgaggtctc
     1381 gacgggcaga cccgcgcgta aagcggcaat ccgcgccacg ttcatcccct gttcgccctc
     1441 cggcatggca cagcccagaa tcacgtcgtc gatctgcttg ggattgagct gcggcacccg
     1501 cgccag
//
LOCUS       DATLJT010000546         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1640708,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000546 DATLJT010000000
VERSION     DATLJT010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640708"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VM910_12610"
     CDS             complement(<1..721)
                     /locus_tag="VM910_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HVI63408.1"
                     /translation="MHRSSESIGTIAAALAKAQAELTNPEKSLIATIRSPFSREGDRT
                     FRYAPLSSGLDIVRKGLGRYEIATIQTTAIDKDAGLLRLTTVLAHSSGEWISSEWPVC
                     PIADIASAQRMGAALTYARRYALFTLVGIAGEDDLDAPDLGATSKTGVVEPPRSDHRS
                     QSTGDAAVAVRARDGAKLRVPSGGTVLTSDQSAILRERLLGQVVAINSTDEAAAWARQ
                     NLSAKNTLTVWDARIVEEEFQT"
     gene            complement(705..1547)
                     /locus_tag="VM910_12615"
     CDS             complement(705..1547)
                     /locus_tag="VM910_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HVI63409.1"
                     /translation="MTLLFETAGISPKRLNRRGFIGGSDARVIMGSDEAALLQLWREK
                     RGEAEPEDLSDNLIVQLGLVTEPLNRHWFERNTGNVITQVQQRVQHPVIRWMAATLDG
                     MVEQSGAVFEAKFMLPWSFSDKVAAEKYMPQLQHNMWVTNATAAVLSIITGGGKWVEI
                     KISADSLYQHLLLTAEKKFWRCVESGEPPRLFGVDSPRARIEAVRIVDMSASNAWAAF
                     SGVFRRTREAHLKHEHAKTELKALMPEDAKEAVGHGIRAKRSKSGAVSFEVMSMEGSH
                     APLE"
     gene            complement(1544..1921)
                     /locus_tag="VM910_12620"
     CDS             complement(1544..1921)
                     /locus_tag="VM910_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63410.1"
                     /translation="MAQAECITTAIRELMSCGRPPKSTNPVRPAHIEFVAALAGNIPH
                     PIYADVDSEDLDGRADHLEKVFAALHVYLTAIIADTAHNIPGSTLDRRYLDNLFRDLS
                     ADALGVIRNAAEEMREYENWRAS"
BASE COUNT          395 a          614 c          634 g          422 t
ORIGIN      
        1 tcgtctgaaa ttcctcttcg acaattcggg catcccacac tgtaagcgtg ttcttggcgg
       61 aaagattctg acgtgcccag gcagccgcct cgtcggttga attgatcgcg accacttgtc
      121 cgagcaagcg ttcccgaaga attgccgatt gatcggacgt gaggacggtt cctccggaag
      181 gcacacggag ctttgcgcca tcgcgagccc gtaccgcaac tgctgcatcg ccggttgatt
      241 ggctgcgatg atcggatcgg ggcggctcca caactccggt tttggacgtt gcaccgaggt
      301 ccggcgcgtc aagatcatct tccccagcaa tgccgacgag cgtgaacagc gcatagcgcc
      361 gggcataggt gagtgccgct cccattcgtt gtgccgaggc tatatcggcg atcggacaca
      421 caggccattc cgaggagatc cattcacccg acgaatgagc aagcacagtt gtgaggcgaa
      481 gaagcccggc atctttgtcg attgcggtgg tctggattgt cgcgatctca tagcggccaa
      541 ggcccttgcg gacaatgtcc agaccgctcg ataacggggc gtagcggaag gtgcggtctc
      601 cctcacggga aaagggggat cggatggtcg caataagtga cttctctgga ttggtcagtt
      661 ccgcttgcgc cttggcaagt gctgccgcga tggtgccgat ggattcactc gagcggtgca
      721 tggctgccct ccatgctcat gacctcgaag ctcaccgcac cggatttaga tcgttttgct
      781 cggatgccat ggccgactgc ttccttggcg tcctcgggca taagggcctt gagctccgtc
      841 ttggcatgct catgcttgag gtgagcctcg cgcgtgcgcc gaaacacgcc agagaacgcc
      901 gcccaggcgt tggacgcact catgtcgacg atccgcaccg cttcgatccg cgcccgcggt
      961 gaatcgacgc cgaagaggcg agggggctca ccactctcga cgcagcgcca gaatttcttt
     1021 tccgccgtca gaaggaggtg ctggtaaagc gaatcggctg atatcttgat ctcgacccat
     1081 ttgccgccgc cggtgataat cgagagcacc gcggccgtgg cattggtcac ccacatattg
     1141 tgctggagct gcggcatgta tttttcggcc gcgaccttgt ccgaaaacga ccacggcagc
     1201 atgaactttg cctcgaagac ggcgccgctc tgctcaacca ttccgtcgag cgtcgccgcc
     1261 atccagcgga tcactgggtg ctggacccgc tgctgcactt gcgtaatgac gttcccggta
     1321 ttgcgctcga accaatgccg attgaggggc tcggtcacca ggccaagctg gacgatgaga
     1381 ttgtcggaga ggtcctcagg ctcggcctcg ccgcgtttct cgcgccacag ctgcagcaag
     1441 gcagcctcat cgctccccat gatcacccgg gcgtcggagc cgccgatgaa gccccggcga
     1501 ttaaggcgtt tgggagaaat ccctgcagtc tcgaacagga gggtcatgac gccctccaat
     1561 tctcatattc gcgcatttcc tccgcggcat tgcggatgac gcccagcgcg tcggccgaca
     1621 agtcccggaa caggttgtcg aggtagcggc gatcgagcgt actaccgggg atgttgtgcg
     1681 ccgtgtcggc gatgatcgca gtgagataaa cgtgcagcgc cgcgaatacc ttctccagat
     1741 ggtcggcgcg gccgtcgaga tcctccgaat cgacatcggc gtagatcggg tgcggtatat
     1801 tcccagcgag ggctgcgacg aattcgatgt gcgccggccg caccgggttt gtggacttcg
     1861 gcggccgtcc acaggacatc agctcgcgga ttgcggtagt aatgcactca gcctgggcca
     1921 tgggttacct ccgtggcttg ggttagggcc ggtgcgaagt ttgccgcttc tcatcggccc
     1981 gcaaatttta ggtaatacct agccatggcc aaagtgtcaa gcaaaaaggt aatacccaaa
     2041 aaacgtgggc gcccggggac cggta
//
LOCUS       DATLJT010000547         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_720988,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000547 DATLJT010000000
VERSION     DATLJT010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720988"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM910_12625"
     CDS             complement(<1..206)
                     /locus_tag="VM910_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63411.1"
                     /translation="MSKPRISYVDPAKVEDAAMRAEFDRCAREGTPRPESQAIRAHVP
                     AVFWSFANSWRDVFKNGVADHGIK"
     gene            557..1126
                     /locus_tag="VM910_12630"
     CDS             557..1126
                     /locus_tag="VM910_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI63412.1"
                     /translation="MGARAARVLLATAACLLWLNGCESSTRLGDLFQSKADTAATAQD
                     DPITTGSVRPTPRDPPAAAKAAPDDELKLGKKYFQAGNFSLAERHFRRAVELHPRDLE
                     SWIGLAASYDRQRRFDLADRAYDQATKIAGPTAEILNNRGYSYMLRGDPRRARDTLLE
                     AQARDPGNTYIKNNLETVEASLRKSKATQ"
     gene            complement(1186..2277)
                     /locus_tag="VM910_12635"
     CDS             complement(1186..2277)
                     /locus_tag="VM910_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI63413.1"
                     /translation="MNTRRDFLKGAGAATGIVFCSCGPRDAAWAQQGDGQRLPVMVNG
                     KRIKTIDVHAHCHFREAVVLMGDEASRVLPRVRGAQEHFIVIEERLKGMDAQAIDMEV
                     LSINPFWYRKDRDTAAQIVKVQNEKLAELCASRPDRFAAFASLALQYPDLAAQQLETA
                     VKKQGLRGAAIGGSVLGEDFSEPKFHPVWAKAEELGAVLFIHPQSTPDLARRFKGNGW
                     LANTIGNPLDTTIALQHLIFEGTLDRFPGLKIIAAHGGGYLASYAARSDHACFVAPQN
                     CNPEIKLKKKPTEYLNQLYFDAVVFTPEALRHLVAQVGVSQVMLGSDHPIPWEPHPVD
                     HVFATATLTDDEKMAILGGNAARLFGMNG"
     gene            2505..3329
                     /locus_tag="VM910_12640"
     CDS             2505..3329
                     /locus_tag="VM910_12640"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI63414.1"
                     /translation="MRKSCVPIAAIMLVTGITAACADPPVDKPVTKKDPALDALVSSD
                     AKLEEISTGYGFTEGLVWVPKGKGGYLLLSDMPANVIYKLSTDGKEKSLYLDHSGYTG
                     FDIWRVGFMQTNGRSKDDPRYEEFPMIGSNGLALDREGGKTAAVIRDIPHTNGLAFSP
                     DEKILYANGSRERFVRAYDVQADGTLTNSRLLIDLNSDPRPGITDGMKVDTMGNIWES
                     GPGGIWILSPQGTVLGHIQVPELVANVAFGDPHYKTLYIAARNSVYKIRTNVAGIP"
BASE COUNT          681 a         1150 c         1071 g          657 t
ORIGIN      
        1 tccttgatgc cgtggtcggc gacgccgttc ttgaacacat cgcgccacga attggcgaac
       61 gaccagaaca cggccggcac atgggcgcgg atcgcctggc tttccggacg cggcgtgcct
      121 tcgcgggcgc agcggtcgaa ctcggcgcgc atggcggcgt cttcgacctt ggcggggtcg
      181 acgtaactga tccgtggctt tgacatcgat gcttctccgc tttcttctgc gcctcaaaga
      241 tgaggcggat acgctttgtt caccatgccc cgcatgctcg ttcgcatgtt gccgcatggc
      301 cacaatgcga tcaaccgcgc tcgcgcgttg ttgaccgcgc ggttggtgcc ccgaaatcgc
      361 tgtttcccgc cgcgcgcgca tggtcgatgg ctcgcgcggg cgcggcgcag gcccgccgcc
      421 ccgcttgcgc ctggcgaggg attcttaacc atcctcacct agcttggcgc tacgggtttg
      481 tggggatggc cgcagcgtcg cgtgttttcg cctcgacgag cgcgatccga tgcttggccg
      541 ccgggggaac acgccaatgg gagcgagagc tgctcgagtc ctgctcgcga cggccgcgtg
      601 ccttctatgg ctcaacggct gcgaaagctc gacccggctc ggcgaccttt tccagtcgaa
      661 ggccgacacg gccgcaacag cccaagacga tccgatcacc accggcagcg tgcggccgac
      721 gccgcgcgac ccacccgccg ccgcgaaggc ggcgcccgat gacgagctca agctcggcaa
      781 gaaatacttc caggcgggca atttcagtct ggccgaacgt catttccgcc gcgcggtaga
      841 gctgcatccg cgcgacctcg aatcctggat cggcctggcc gcctcgtacg accgccagcg
      901 ccgcttcgat ctcgccgatc gcgcctacga ccaggcgacc aagatcgcag gccccacggc
      961 ggagatcctc aacaaccgtg gctactccta catgctgcgc ggcgacccgc ggcgcgcccg
     1021 cgacacgctg ctggaggcgc aggcccgcga ccccggcaac acgtacatca agaacaacct
     1081 tgaaacggtc gaggcgagcc tgcgcaaaag caaggccacg cagtaggctc ttgcgaaatc
     1141 atcggctcgt tgtcttgcgc aaggacgaca gcgcccgagg cgctcctaac cattcatccc
     1201 gaataggcgc gccgcgttgc cgccgaggat cgccatcttc tcgtcatcgg tcagcgtcgc
     1261 agtagcgaag acgtggtcca caggatgcgg ctcccacggg atcggatggt cgctgccaag
     1321 catgacttgg ctcacgccaa cttgcgccac gagatggcga agtgcctcgg gggtaaacac
     1381 aacggcatcg aaatagagct gattgaggta ttcggttggc ttctttttca gcttgatctc
     1441 gggattgcag ttctgcggcg cgacgaaaca ggcgtgatct gagcgcgcag cataggacgc
     1501 gagatagccg ccgccgtgcg cagcaatgat ctttaggccg ggaaagcgat ccagcgttcc
     1561 ctcaaagatc aaatgttgta gagcgatggt cgtgtcgagc gggttgccga tcgtgttcgc
     1621 cagccagccg ttacccttaa agcgccttgc caaatccggc gtgctttggg ggtgaatgaa
     1681 cagcactgct ccaagctcct ccgcctttgc ccataccggg tggaacttgg gttcggaaaa
     1741 gtcctcgccc aacacgctgc cgccgatcgc agcgccgcgt aggccctgct tcttcactgc
     1801 ggtctcgagc tgctgcgccg ccaggtccgg atattgcagg gcgagcgagg caaaggcggc
     1861 gaagcggtcc ggacgcgaag cgcacaactc ggccagtttt tcgttttgga ccttgacgat
     1921 ttgggcggcg gtgtcgcggt ccttgcgata ccagaatggg ttgatggaga gaacctccat
     1981 gtcgatcgct tgagcatcca tgcccttgag gcgctcctcg atgacaatga agtgctcctg
     2041 cgcacctcta acccgtggca gcactcggct ggcctcatcg cccatcagga caacggcttc
     2101 gcggaagtgg caatgtgcgt gcacgtcaat ggtcttgatg cgcttgccat tgaccatcac
     2161 tggcaggcgt tgtccgtcgc cctgctgagc ccacgccgca tcccgcggcc cacaactgca
     2221 gaaaacgatg ccggtcgcgg cgccggcacc cttgagaaaa tctctgcgcg tgttcatgtc
     2281 cggctcccag ttgaccgttc gaccgaatgt cccgaacaac gggacgtcat ccgtagtctc
     2341 gcaatttcat tcgtctgccc caacaaattc aaggccagcc ataccagcgc cgccgtgccc
     2401 acagcggtgc cgcacgttcc catgctggaa gggcctcgtc ggagccgcta ggcttgcggc
     2461 cgaaacgtcg ctcacctgag aagcgacacc cagggaggtc gccgatgagg aagtcttgcg
     2521 ttcccattgc cgccatcatg ctggtgacgg gaatcaccgc ggcatgcgca gacccgccag
     2581 tcgacaagcc agtcaccaag aaggatccgg cactggacgc gctcgtgtcc tccgacgcca
     2641 aactcgaaga gatcagcact ggatatggat tcacggaggg gctggtctgg gttccgaaag
     2701 gaaagggcgg atatctgctg ctgagcgata tgcccgccaa cgtgatctac aaactctcga
     2761 ccgatggcaa ggagaagtcg ctctacctgg atcatagcgg ctataccggt ttcgacatct
     2821 ggcgcgtcgg cttcatgcag accaatggcc gcagcaagga cgatccgcgc tacgaggaat
     2881 tccccatgat cgggtcgaac ggacttgcgc tcgaccgcga gggtggcaag actgccgcgg
     2941 tgatcaggga cattccccat accaacggcc tcgccttctc acccgacgag aaaatcctct
     3001 atgccaacgg cagccgcgaa cgtttcgttc gcgcctatga cgtccaggcc gacggcacgc
     3061 tcaccaacag ccggttgttg atcgatctca acagcgatcc acggcccggc atcaccgatg
     3121 gcatgaaggt cgacaccatg gggaatatct gggaatccgg acctggcggt atctggattc
     3181 tttcgcccca gggcacggtt ctgggtcaca tccaggtgcc ggaactggtc gccaacgtag
     3241 cgttcgggga tcctcactat aagacgctct acatcgcggc gcgcaacagc gtttacaaaa
     3301 tccggacgaa tgtcgcaggc attccatagg ctcgatctcg cgtcctgcag gtgccgtttc
     3361 aggacatcaa ccgagcgcgc ggttcaagcg cagatccgca gcgccgatgg tcgcgcgccc
     3421 cctagacttt gcggaggcgc catgcaacgt ggcgccgtcc agcggttcac ggcgtcatgt
     3481 tcgatagctt tcgcaaattt ctatccgagg tatcggaagg cggcgagcac ccgatccact
     3541 tcgagcatga cgattaccg
//
LOCUS       DATLJT010000548         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1870235,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000548 DATLJT010000000
VERSION     DATLJT010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870235"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VM910_12645"
     CDS             complement(<1..486)
                     /locus_tag="VM910_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVI63415.1"
                     /translation="MSRIPNFADVAFAPEAAAPLAGSAEPWLTPEGIPVKPAYGEADL
                     AGVDFLETWPGIAPYLRGPYPTMYVNQPWTIRQYAGFSTAEDSNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDHPRVSGDVGMAGVAIDSIYDMRTLFAGIPLDKMSVSMTMNGAV
                     LP"
     gene            complement(563..1027)
                     /locus_tag="VM910_12650"
     CDS             complement(563..1027)
                     /locus_tag="VM910_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI63416.1"
                     /translation="MSAETKPLLTGGCQCGAIRFALTAAPVKISICHCRMCQKASGAP
                     FASFADIEKSDFAWTRGKPAAFKSSTIAERDFCRDCGTPLSFRRIDGERIEIMTGTFD
                     RPDRVIPTRQYGTESRLGWVVGIANMPSQTTLQNYGPEKVATIVSHQHPDHD"
     gene            complement(1024..2898)
                     /locus_tag="VM910_12655"
     CDS             complement(1024..2898)
                     /locus_tag="VM910_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVI63417.1"
                     /translation="MTSVTDDLRLGAEFPAATYDDWRKLVDGVLKGASFEKLVSKTYD
                     NVKIAPIYTRAKGATPLPGRAAAAPWQIMQRVDHPDPAKANAFLLQDLENGATGLEIE
                     FQGGPGARGFGVADATPETLARLFDGVVLDAGISISLNPVFGRQNAGVNLAGLAESRK
                     LDPATLDIRFNYQPLSTMATRGAAPEAWSEFAGPFVQMIGELMQRGFRAPFVLADGRP
                     VHDAGGSEAQELAFALALAVDYLRALESGGIALDRARAAISFRLVADADQFLTIAKFR
                     ALRLLWSRVEAACGLAPKPLFVAAESAWRMLTQRHPHVNMLRATMATFAAGLAGANAV
                     TVLPHTLALGLPDAFARRVARNTQLILLEESNLAKVADPAAGSGGIESLTKDLCEAGW
                     SLFQAIEKSGGAFAALQQGLIQDKVETTRKARAANIAKRRDVLTGASQFPNLHEAHVA
                     VEDVKAVVPAAETNAAITFAPLTATRLAAPFERLRDRSDRMLNDRGKRPTVFLANLGR
                     HSDFTARATFAKSFFEAGGIEAIDSEGFSDPAALARAYTASKADLVCLCSSDKVYAER
                     GPEAARALQNAGARHIYLAGRPGDREAALKTAGVGAFIRAGDDALAALQDAYRQMEQP
                     "
     gene            complement(2989..3480)
                     /gene="folK"
                     /locus_tag="VM910_12660"
     CDS             complement(2989..3480)
                     /gene="folK"
                     /locus_tag="VM910_12660"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HVI63418.1"
                     /translation="MADVLIALGGNVGDVRATFRKAIANICGMAQAALVARSSDYATP
                     PWGNEQQARFINACIEIETSLDPHALLFTLQRIEQKFGRDRSKETRWGPRTLDLDLLA
                     YDDVCLQRPELTLPHPHLFERAFVLVPLVEIAPDRIIAGRSVRQALAGLSTEGIERLP
                     DMV"
     gene            complement(3473..3853)
                     /gene="folB"
                     /locus_tag="VM910_12665"
     CDS             complement(3473..3853)
                     /gene="folB"
                     /locus_tag="VM910_12665"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HVI63419.1"
                     /translation="MTDTIFITGVVVHARHGVMEHETEVGQRFVIDLELFSDLSDASR
                     SDRLADTVSYSQVVSTATAAFRNANYKLLERAAGAVADAILAAFPRIRAVKVTVHKPH
                     APIAEIFEDVGVVLTRNRHPPQHG"
     gene            complement(3850..>4515)
                     /gene="folP"
                     /locus_tag="VM910_12670"
     CDS             complement(3850..>4515)
                     /gene="folP"
                     /locus_tag="VM910_12670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HVI63420.1"
                     /translation="ADIIDVGAESTRPYGGMQPIPAEQEVERLQPVLAQIVALGVPVS
                     IDSMKPEVTAFALDRGAVIVNDVWGMQRDRGMAELVAARNCPVILMHNRDQADPAIDI
                     MRDIAAFFARSLDIAAKAGIPKDNIVLDPGIGFGKTAVQSLTALARLSELASFGLPLL
                     VGASRKSFIASVTPSEPHQRLGGSVAAHLLAARQGARIIRTHDVAETVQALKVASAIE
                     AKT"
BASE COUNT          805 a         1410 c         1506 g          794 t
ORIGIN      
        1 cggcagcacg gcgccgttca tggtcatcga cacgctcatc ttgtcgagcg ggatgccggc
       61 aaacagcgtg cgcatgtcgt agatcgaatc gatcgcgaca ccggccatgc cgacgtcgcc
      121 ggagacgcgc ggatgatctg agtcatagcc gcgatgggtg gcgagatcga aggcgaccga
      181 aagccctttc tgcccggcag cgagattgcg ccgatagaac gcgttggaat cctcggccgt
      241 ggagaagccg gcatattgcc tgatcgtcca gggctggttg acatacatgg tcgggtaggg
      301 gccgcgcaga tatggcgcaa tccccggcca ggtctcgagg aaatcgacgc cggcaagatc
      361 ggcttcgcca taggccggct tgaccggaat gccctcgggc gtgagccagg gctctgcgct
      421 gcctgcaaga ggtgcggcag cctcgggcgc gaacgcgaca tcagcgaaat taggaatgcg
      481 gctcattccc tggtttcctc aaactggcga acgatctcat tctagcacgg ccaaaaccta
      541 tgataagccg ccgggaacct cgttaatcat gatccggatg ctggtggctg acgatggttg
      601 ccaccttttc cggcccgtag ttctgcaacg tggtctggct cggcatgttc gcgatcccga
      661 ccacccagcc gagccgtgat tccgtgccat attgccgcgt cgggatcacc cggtccggcc
      721 gatcgaaggt gccggtcatg atctcgatcc gctccccgtc gatgcggcgg aagcttaagg
      781 gcgtgccgca atccctgcag aaatcgcgtt cggcaatcgt cgaggatttg aacgccgcag
      841 gcttgccgcg cgtccaggca aaatcgctct tttcgatgtc ggcaaaggag gcgaacggcg
      901 cgccggatgc cttctggcac atccggcaat ggcagatgct gatcttgacc ggcgcggccg
      961 tcagcgcgaa gcggatggcc ccgcattggc agccgccggt gagcaaaggt ttggtctcgg
     1021 cgctcatggt tgctccattt gcctgtaagc atcctgcagc gcagcgagcg catcgtcgcc
     1081 cgcacggatg aaggcaccga cgccggccgt cttcagcgca gcctcccgat cgcccgggcg
     1141 tcctgccaga tagatatgtc tggcgcccgc gttctgcagc gcccgcgcag cctccggacc
     1201 gcgttcggca tagaccttgt cggaggaaca gaggcagacc aggtcggctt tggacgccgt
     1261 gtaggctctg gcgagcgcgg cgggatcgga aaatccctcg ctgtcgatgg cctcgatgcc
     1321 gccggcttcg aagaagcttt tcgcaaaggt tgcacgcgcg gtaaagtcgg agtgacgccc
     1381 gagattggcg aggaagacgg tcggccgctt tcccctgtcg ttcagcattc gatccgaacg
     1441 gtcacgcaag cgctcgaacg gtgcggcgag ccgcgtggcc gtgagcggcg cgaacgtgat
     1501 ggcagcattg gtctcggctg ccggaacgac cgccttcaca tcttcgacag cgacgtgcgc
     1561 ctcgtgcagg ttgggaaatt ggctggcgcc ggtcagcaca tccctgcgct tggcgatatt
     1621 cgccgcgcgt gccttgcgcg tcgtctcgac cttgtcctgg atcaggccct gctgaagggc
     1681 cgcgaacgcg ccgcccgact tttcgatggc ctgaaacagc gaccagccgg cttcgcagag
     1741 gtccttcgtc aggctttcga tgccgcccga acccgcggcg ggatccgcta ccttggcgag
     1801 gttggattct tccaggagga tgagttgcgt gttccgcgcc acgcgccgtg cgaacgcatc
     1861 cggaagtccc aatgccagcg tatgcggcag cacggtaacc gcattggctc cggcaaggcc
     1921 cgcggcaaag gtcgccatgg tcgcgcgcaa catgttcacg tgggggtggc gctgcgtcag
     1981 catgcgccat gccgactccg cggcaacgaa gagcggcttc ggcgcaagcc cgcaggcggc
     2041 ttccacgcgg gaccacaaca gcctgagcgc gcggaatttc gcgattgtca ggaactgatc
     2101 ggcatccgcc acaagccgga aggagatcgc cgcgcgagca cggtccagcg cgatgccgcc
     2161 gctttccagc gcgcgcagat agtcgaccgc aagcgcgagt gcaaacgcca actcctgcgc
     2221 ttccgatccg ccggcgtcgt gcacgggacg gccgtcggcg agcacaaacg gtgctcgaaa
     2281 gccgcgctgc atcagttcgc cgatcatctg tacgaacggc ccggcgaact cggaccacgc
     2341 ctcgggcgcg gcgccgcgcg tcgccatggt ggaaaggggt tgatagttga agcggatgtc
     2401 gagtgtcgcc ggatcgagct tcctggattc ggcgagaccg gcaagattga cgccggcatt
     2461 ttgccggccg aacaccggat tgagcgaaat cgagattccg gcatcgagga cgacgccgtc
     2521 gaacaggcgc gccagcgttt caggcgtcgc atcggccacg ccaaagccgc gcgcgccggg
     2581 tccaccctgg aactcgattt cgagaccggt cgcgccattt tcgagatctt ggagaagaaa
     2641 ggcgttggcc ttcgccggat cggggtgatc gacccgctgc atgatctgcc acggcgcggc
     2701 ggcggcgcgc ccggggaggg gggtggcgcc tttcgcgcgc gtatagatcg gcgcgatctt
     2761 gacgttgtca taggtcttgc tgacgagctt ttcgaacgat gcgcccttca acacgccatc
     2821 gaccagcttg cgccagtcat cgtaagtcgc ggccggaaac tcggctccga gccgcaggtc
     2881 gtcggtcaca gaggtcattt cggcaggcta ctcccgaggc gcagtcttct gctgttgggc
     2941 cgcaaattgc cacgggtgga gcgccaagcc aaaccttgtt ttggcgtctc aaaccatatc
     3001 cggcaaccgt tcgatgcctt cggtcgacag gcccgcgagc gcctgccgta cgctgcggcc
     3061 ggcgatgatg cgatcgggcg cgatttcgac cagcggcacc agcacgaaag cgcgctcgaa
     3121 caggtgcgga tgggggagcg tcagctccgg tcgctgcaga cagacgtcgt cataagcgag
     3181 cagatcgagg tcgagggtgc gcggacccca gcgcgtttcc tttgagcggt cgcggccgaa
     3241 tttctgttcg atccgctgca gcgtgaacag gagcgcatgg ggatcgaggc tggtctcgat
     3301 ctcgatgcag gcattgatga agcgcgcctg ctgctcgttg ccccagggcg gcgtcgcgta
     3361 atcggaggag cgcgccacga gtgctgcctg cgccatgccg cagatattcg caatcgcctt
     3421 cctgaacgtc gcgcgaacgt cgccgacatt gccgccaagt gcgatcaaga catcagccat
     3481 gctgcggcgg atggcggttg cgcgtcagca ccacgcccac gtcctcgaag atttccgcga
     3541 tcggcgcgtg cggcttgtgg acggtgacct tgacggcgcg gatgcgtgga aaggccgcaa
     3601 ggattgcatc ggcaaccgcg ccggcggcgc gttccagaag cttgtaattg gcgttcctga
     3661 acgctgcggt cgcggtcgaa accacctggg aataggaaac agtatcggcg aggcgatcgg
     3721 agcgcgaggc gtccgacaag tccgaaaaca gttcgagatc gatgacaaaa cgctgcccga
     3781 cctcggtctc gtgctccatc acgccatggc gggcatggac aacaacgccg gtgatgaaga
     3841 tcgtgtctgt catgtctttg cctcgatcgc ggatgcgacc ttgagagcct gcacggtctc
     3901 cgcgacatca tgggtgcgga tgatgcgcgc gccctgccgg gctgcgagca gatgcgcggc
     3961 gacggagccg ccgagccgct ggtgcggttc cgatggcgtg acgctcgcaa tgaagctctt
     4021 gcgggaggcg ccgaccagca gcggcaggcc gaagcttgca agctcgctca gtcgcgcgag
     4081 cgcagtcagg ctctgcacgg ccgtcttgcc aaaaccgatg ccgggatcga gcacgatgtt
     4141 gtccttgggg atgcctgcct tggccgcgat gtcgagcgag cgcgcaaaga aggcggcaat
     4201 gtcgcgcatg atgtcgatgg cggggtcggc ctgatcgcgg ttgtgcatca ggatcacggg
     4261 gcagttgcgc gcggccacca gttcggccat gccgcggtcg cgctgcattc cccagacgtc
     4321 attgacaatg actgcgccgc gatccagcgc gaacgccgtg acctccggct tcatgctgtc
     4381 gatggagacg ggaacgccga gcgcaacgat ttgcgccagc acaggctgca ggcgttccac
     4441 ctcctgttcg gccgggatcg gctgcatccc gccgtaaggg cgggtcgatt cagcgccgac
     4501 gtcgatgata tcggc
//
LOCUS       DATLJT010000549         3373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4168592,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000549 DATLJT010000000
VERSION     DATLJT010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168592"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VM910_12675"
     CDS             <1..1485
                     /locus_tag="VM910_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HVI63421.1"
                     /translation="GVQALMPPQGLLLIAAYLPKSWPVRFVDENIRAATREDFEWAEV
                     VFVSGMHIQRKQMNDICHRAHAYDLPVALGGPSVSACPDYYPAFDYLHMGELGDATDE
                     LIARLARDRSRPAAQVVLKTDDRLPMTEFPVPAYELAELNKYFLANIQYSSGCPYQCE
                     FCDIPGLYGRNPRLKTPKQVIAELDKLREGGHTNTVYFVDDNFIGNRKAALELLPHLV
                     EWQKKNNYPTRLACEATLNIAKRPEILEKMRDAYFITIFCGIETPDPDALIAMQKDHN
                     MMVPILEGVRTINDYGMEVVSGIIMGLDTDKPDTGNALLEFVEQSQIPMLTINLLQAL
                     PKTPLWDRLERENRLNHDENRDSNVEFLLPYDHVIASWRRCMQIAYSPEKVYERYQHQ
                     VDFTYCNRVKPPVTREQLNWANVKRGLVMLRNVIWKIGVHGDYRKVFWKFALRCLRRG
                     DIESLLGASILAHHLISFAREASGGRQNASNYSIRLRGAQVPAE"
     gene            1493..2284
                     /gene="eutC"
                     /locus_tag="VM910_12680"
     CDS             1493..2284
                     /gene="eutC"
                     /locus_tag="VM910_12680"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HVI63422.1"
                     /translation="MSLPRAPTRSSVTDLRSLTPARVALGRSGASLPTRALLDFTLDH
                     ARARDAVHAVFDVPALVAGLTDLGLRPLHVASRACDRQDYLKRPDLGRRLDQPSARLL
                     AEREASSSGRLAIVIGDGLSPAAVHAHAVALMRKLLRQLADAGLDLGDVVVASGARVA
                     LGDEIGALLKASMVAVLIGERPGLSAPDSLGAYLTFAPEIGRTDAERNCVSNIHRAGL
                     AYDEAAFKIAWLVREGLARKKTGVALKDESGEGTARPIADDSRPK"
     gene            2502..>3373
                     /locus_tag="VM910_12685"
     CDS             2502..>3373
                     /locus_tag="VM910_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVI63423.1"
                     /translation="MLEKTRDNEIHVEMVEAPPAKSIAFGIERLGLIPMKAPILSCII
                     LVGLIIGAIFGIDRIKIDDSLSQLFRSNTKEFKQYEEVTKKFPAEEFDVLVVVEGKSL
                     LERDNLEKLRDFVTDLQLVEGTRGLVSLFSARQAPAPGKLPAALFPAELPEGADYDKF
                     IETVKSNEIIRGKLLSEDGTLALIVLSLEPEVVASSKLGKVVGDIRKLMKEDLGNTGL
                     NFELSGVPVMQLEIRNAVERDGLTYNILGILAGCIIAIIFFRKISFMIVAAFPPLIAI
                     LLALGGLGWANFNL"
BASE COUNT          650 a         1071 c         1024 g          628 t
ORIGIN      
        1 ggcgttcagg ccttgatgcc gccgcagggc ctgttgctga tcgcggccta tctgccgaaa
       61 agctggccgg tccgcttcgt cgacgaaaat atacgcgccg cgacccgaga agatttcgaa
      121 tgggcggaag tggtcttcgt cagcggcatg cacatccagc gcaagcagat gaacgacatc
      181 tgccatcgcg cgcatgccta cgacctgcct gtggcgctcg gcggtccctc ggtctcggct
      241 tgcccggact attatcccgc cttcgattat ctgcacatgg gggaactcgg tgacgccacc
      301 gacgaactga ttgcgcgatt ggcgcgcgac cgctcgcgcc cggctgccca agtggtgctg
      361 aagaccgacg atcgcttgcc gatgaccgag tttccggtcc ccgcctacga acttgccgaa
      421 ctgaacaaat atttcctcgc caacatccag tattcgagcg gctgtcccta tcagtgcgag
      481 ttctgcgaca tccccgggct ctacggacgc aatccgcgcc tcaagacgcc caagcaggtc
      541 attgccgaac tcgacaagct gcgcgagggt ggacacacca acacggtcta tttcgtcgac
      601 gacaatttca tcggcaaccg caaggccgcc ctggaattgc tgccgcacct cgtcgaatgg
      661 caaaagaaga acaactaccc gacgcggctt gcctgtgagg cgacgctcaa tatcgccaag
      721 cgtccggaaa tcctcgagaa gatgcgcgat gcctacttca tcacgatctt ctgcggcatc
      781 gagacgccgg accccgacgc gctgatcgcc atgcagaagg accacaacat gatggtcccg
      841 attttggagg gcgtgcgcac catcaacgac tacggcatgg aggttgtctc cggcatcatc
      901 atgggcctcg acaccgacaa gcccgacacc ggcaatgcgc tgctggagtt cgtcgagcag
      961 tcccagatcc cgatgctgac gatcaatctg cttcaggcgt tgcccaagac accactttgg
     1021 gaccggctgg agcgcgagaa ccggctcaat cacgacgaaa accgcgattc caacgtcgag
     1081 ttcctgctgc cttacgatca cgtcatcgca tcatggcggc gatgcatgca aatcgcctat
     1141 tcgccggaga aggtttacga gcgctatcag caccaggtcg acttcaccta ttgcaaccgc
     1201 gtgaagccgc ccgtcacccg cgagcaattg aactgggcca atgtcaaacg cggcctcgtc
     1261 atgcttcgca acgtcatctg gaagatcggc gtgcacggcg actacaggaa ggtgttttgg
     1321 aagttcgcgc tgagatgcct gcggcgaggc gatatcgaaa gcctgttggg cgcgtcgatt
     1381 cttgcccatc acctgatctc attcgcgcgc gaggcctcgg gcggcaggca gaacgcctcg
     1441 aactattcga tcaggttgcg cggggcgcag gtcccggccg agtgaggtgc cagtgagcct
     1501 gccgcgggcg ccgacgcgtt catcagtgac cgatctgagg tcgctcacgc cggcacgcgt
     1561 cgcgcttggc cgctcagggg ccagcctgcc gacgcgggcg ctgcttgatt tcaccctcga
     1621 ccacgcgcgc gcccgcgacg cagtgcacgc cgtcttcgat gtgccggcgc ttgtcgctgg
     1681 cctgaccgat ctcggccttc gcccgcttca cgtggcaagc cgtgcctgcg accggcagga
     1741 ttatctcaag cgccccgatc ttgggcgcag gctcgaccag ccttccgcgc ggcttctggc
     1801 agaacgcgag gcgtcgtcgt caggccggct tgcgatcgtg atcggcgatg gtctttcgcc
     1861 ggcagccgtg catgctcacg cggtcgcgct gatgcgcaag ctgctgcgac aactggctga
     1921 tgcggggctg gacctcggcg acgtggtcgt agcctccggc gcgcgggtcg cgctgggcga
     1981 cgagatcggc gccctgctga aggcaagcat ggtcgcggtg ttgatcggcg agcgcccggg
     2041 tctgtccgcg cccgacagtc tcggcgccta tctgaccttt gcgcccgaaa tcggccgcac
     2101 cgacgccgag cggaactgcg tgtcgaatat tcatcgtgcg ggactcgcct atgacgaggc
     2161 ggccttcaag attgcctggt tggtccgcga gggcctcgcg cggaaaaaaa ccggcgtcgc
     2221 attgaaggat gaaagcggcg agggaacagc gcggccgatc gccgatgatt cccgccccaa
     2281 gtgaaaaatc cgccgctttg aaggcgggaa actgttgaag agcggcaagt gaggggggta
     2341 gccgtgcttg cgggggccac ttcagacctg ctaaacccct gatgcaattt tccgtatgtt
     2401 ttcaagggtt agcgctgttc aaatggggcc gatagcggcc cattcgcacc cttcgaacct
     2461 aagcgatacc gttagaagcc gacgcgagaa ctggacaagg tatgctcgaa aaaacccgcg
     2521 acaatgaaat tcatgtcgag atggtcgagg cgccgccagc caagagcatc gccttcggca
     2581 tcgagcggct cggcctgatc ccgatgaaag cgccgatcct gtcctgcatc atcctggtcg
     2641 gcctgatcat cggggccatc ttcggcatcg accggatcaa gatcgacgat tcgctctcgc
     2701 aactgttccg ctcgaacacc aaggaattca agcagtacga agaggtcacg aagaagttcc
     2761 cggccgagga attcgacgtg ctggtcgtgg tcgaaggcaa gagccttctg gagcgcgaca
     2821 atctcgagaa gctgcgcgat ttcgtcacag acctgcaact cgtcgagggt acccgcggtc
     2881 tggtctcgct gttctcggcg cgccaggcgc cggctcccgg caagctgccg gccgcgctgt
     2941 tcccggccga actgccggag ggcgccgact acgacaagtt catcgagacg gtgaagagca
     3001 acgagatcat ccgcggcaag ctgctgtcgg aggacggcac cctggcgctg atcgtgctct
     3061 ccctcgaacc ggaggtggtc gcctccagca agctcggcaa ggttgtcggg gatatccgca
     3121 agctgatgaa ggaggatttg ggcaacaccg gcctgaactt cgaactgtcg ggcgttcccg
     3181 tcatgcagct cgagatccgc aacgcggtcg agcgcgacgg cctgacctac aacattctcg
     3241 gcatcctggc cggctgcatc atcgcgatca tcttcttccg caagatatcg ttcatgatcg
     3301 tcgcggcctt cccgcccttg atcgcgatcc tgcttgcgct cggcggtctc ggctgggcca
     3361 atttcaacct caa
//
LOCUS       DATLJT010000550         5588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2790318,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000550 DATLJT010000000
VERSION     DATLJT010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790318"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VM910_12690"
     CDS             <1..610
                     /locus_tag="VM910_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI63424.1"
                     /translation="LYQNGERLNPANGYPMRLLLPGYEGNMNVKWLRRIKLVESPVMA
                     INETKQYTILRPDGKAWQFFFPQEVKSFITHPSPGLNMQGPGFYEISGISFSGNGRIS
                     KVEVSADGGKSWAQAALQAPVLSKAFTRFRIPWNWDGRTAILQSRAMDEAGNVQPTRE
                     AFIAERGPPRGTPNVAAFTMEHCNAITSWGINDKGEVSHVYA"
     gene            597..1133
                     /locus_tag="VM910_12695"
     CDS             597..1133
                     /locus_tag="VM910_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI63425.1"
                     /translation="MSMREMLALATGLALGLSVADAADAPRFGQPITPADLAPWDISI
                     APDGVGLPPGSGTSAQGAKVYAERGCALCHGEKGSGGPSGPLVGGGPLNSPDRDPVKL
                     IGNYWPYATTIFDYTRRAMPWQQPKTLTDDEVYALTAYILALNKIIGENDVMNAETLP
                     KVKMPNRDGFISKYPDKY"
     gene            complement(1328..2479)
                     /locus_tag="VM910_12700"
     CDS             complement(1328..2479)
                     /locus_tag="VM910_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HVI63426.1"
                     /translation="MRSSFRTSVIAATAVVFGFSSAAMAVTKKRPWSDLPAGQCVPEW
                     WLDDPLDPLARLLCNKAVPTGLSFSGSFGYASTASNFSVFDTFLLDRRGDVASGLVTP
                     TGRAGFGGVSLALTSPNLFPVGGAQGRYQQGGIHIGVEGGMDFFGGSSATNQVFPGGG
                     TVSDTISFKPTSLTHVAGVVHIPIGWPESPTWWALSDFYGKVGGGWSRSDLTYNFSML
                     TPTVPAFSATQSTTQAGLYWGVGFGTPLPVKKGWRAPPPILSFEFDQFSTGSRDVIFG
                     APATRQVSATLGPERFNMYRVNLTQPFVASDVRLKRDIVALGRLDNGLTLYRYRYLWS
                     EELFVGVMAQEVAELVPQAVTLRPEGYLAVDYAQLGLRLQTWDEWVSSH"
     gene            complement(2941..3783)
                     /locus_tag="VM910_12705"
     CDS             complement(2941..3783)
                     /locus_tag="VM910_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI63427.1"
                     /translation="MFTHRPRKPRLIVTVGDIVEEQKTGATMIRKHVIRASLLRATLM
                     VGAIAWGSAIATDPAMARNGVAAQPVVPDWSGVYFGAYVGGGFGGNPWNDPDGVGLGR
                     TSLSGAIFSVAVGYNWQIGNVVLGIEGSGGLSTISGDFTARNWNFATRIPGVESAVGR
                     VGFTPGPGANTLLYVKGGAAWAQYKDTSVWPEIGTNFESKRTLGGWTAGAGYAYRFAP
                     NMAITFEYNHYNFGSGRVDLIPDKPDVRPFNVGISNWQINSFSVGFNYQLGGASAAQF
                     PPMR"
     gene            4342..>5588
                     /locus_tag="VM910_12710"
     CDS             4342..>5588
                     /locus_tag="VM910_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI63428.1"
                     /translation="MHKLSFGDAFYAGRLVKLLTFGGLAVLVALECLDATARAQTAER
                     RTDPAIRPEFREPVTLASKEGVLEVRLTARQGQATLDTVATPVQNFLMFDYEVIRGTA
                     SDGRSSGGNLYPAPTLHVFPGETLIVHFENGLTGLTIRDYFIPQYTPKGQPIALYPQQ
                     MTSSPINLHTHGAHISPRGNADNVLLHIPPGMSNTYTYDLPRNMPQGLYWYHSHLHGL
                     TSAHVYTGLVGLLAIGRTDGNLPLVTEKNIPIRDMALQYNFVFDRAGGLAQLNNVSWS
                     QWVSTITPPKPEELANGTYQPSLAPVNFKQSKPGTKYFTVWYAGPLSIHNRRGTFQFI
                     PSNLQHFVAADGKAESDVAADASLPDYQRDVQFTVNGQFQPVIKSKAGQTEIWVLANV
                     SDFAYMNVQLTETATGRHPPIAIV"
BASE COUNT         1155 a         1767 c         1637 g         1029 t
ORIGIN      
        1 gctctaccag aacggcgagc gcctcaatcc ggcaaacggc tatccgatgc gcctgttgct
       61 gccgggctac gagggcaaca tgaatgtcaa gtggctgcgc cggatcaagc tcgtcgaatc
      121 accggtgatg gccatcaacg agaccaagca gtacacgatc ttaaggccgg acggaaaagc
      181 ctggcagttc tttttcccgc aggaggtcaa atccttcatc acgcacccgt ccccggggct
      241 gaacatgcag gggcccggct tctacgagat ctccggcatt tccttttccg gaaatggccg
      301 catcagcaaa gtcgaggtct cggccgacgg cggcaagagc tgggcgcaag cggcgttgca
      361 ggcgccggtt ctgagcaaag cgttcactcg cttccgtatt ccgtggaact gggatggtcg
      421 caccgcgatc ttgcaaagcc gtgcgatgga cgaggccggc aacgtgcagc cgacgcgaga
      481 ggcattcatc gccgagcgcg ggccgccccg gggtacgccg aacgtggcgg cgttcacgat
      541 ggagcactgt aacgccatca ccagctgggg gatcaatgac aagggcgagg tgagccatgt
      601 ctatgcgtga gatgcttgcc ttagccactg ggctcgcgct cggcctcagc gtggccgatg
      661 ccgccgacgc cccgcgattc ggacaaccaa tcacaccggc cgatctcgcg ccctgggaca
      721 tcagcattgc gccggacggc gtcggtctgc cgcccggcag cgggacatcc gcgcagggcg
      781 ccaaggtcta cgccgaacgc ggatgcgccc tctgccacgg cgagaagggc agcggaggac
      841 cgagcgggcc gctggtcggc ggcggtccgc tcaacagccc tgaccgcgat ccggtaaagc
      901 taatcggcaa ctattggcct tatgccacaa ccatcttcga ctacacgcgc cgggcgatgc
      961 cgtggcagca gccgaagaca ctcaccgacg atgaagtcta cgcactgact gcctacatcc
     1021 tcgcgctcaa caagatcatc ggcgagaatg acgtgatgaa cgcggagacg ctgcccaagg
     1081 tgaagatgcc caaccgtgac gggttcatca gcaagtaccc ggacaagtac taatggccag
     1141 agcgcctgat aaaaactcga tctgagcgtg agatgtccgc tttgcccccg aaaccggaca
     1201 aatagcagac gtccgctttg tgccaaaagc ggacatttgc caaattaatc gacccgtcgg
     1261 tgagcgtgtg cagctgccct gggatcgcga gcgccgtgtg aaaagcgtca ctcaccgcgc
     1321 cgtgcactca atgcgaagat acccactcgt cccatgtctg caggcgcaag ccgagctggg
     1381 cgtagtcgac ggccaagtag ccctcgggcc gcagtgtcac cgcttggggc accagctcgg
     1441 caacctcctg cgccatgacg ccgacgaaaa gctcctcgct ccagagatat cggtaccgat
     1501 agagggtaag accgttgtct agccgtccga gcgcaacgat gtcgcgcttc aagcgcacgt
     1561 cggaggcgac aaagggctgg gtgagattca cacgatacat gttgaagcgc tcggggccca
     1621 gggtggcgct cacctgacgc gtggcgggcg caccgaagat cacatccctg ctgccggtcg
     1681 aaaactgatc gaattcgaag ctcaagatcg gcggtggcgc gcgccatccc tttttcacgg
     1741 ggagcggcgt gccaaagcca acgccccaat acaggcctgc ctgggtcgta ctctgggttg
     1801 ccgaaaacgc cggcaccgtc ggcgtgagca tgctaaagtt gtaggtcaaa tcgctgcggg
     1861 accacccgcc gcccactttg ccgtaaaaat cggagagggc ccaccatgtg ggcgattctg
     1921 gccatcctat tgggatgtga acgacaccgg cgacatgcgt cagactggtg ggcttgaagc
     1981 tgatggtgtc gcttaccgtg ccgcccccgg ggaacacctg attggtcgcc gagctgccgc
     2041 cgaagaaatc catgccgcct tccactccga tgtggatacc cccctgctgg tatcgcccct
     2101 gcgccccgcc aactgggaac aagttcggtg acgtgagcgc caaactgacg ccgccgaatc
     2161 ccgcgcggcc ggtaggcgtg accaggcccg acgccacatc acccctcctg tccagcaaaa
     2221 aggtgtcaaa gaccgaaaaa ttgcttgcgg ttgacgcgta gccgaaggag ccggagaagc
     2281 tcagcccggt cggaacggct ttgttgcaca aaagacgggc caacggatca agcgggtcgt
     2341 cgagccacca ctcaggcaca cactggccgg cagggaggtc gctccatggc cgtttctttg
     2401 tcaccgccat cgcggcggat gaaaacccga acacgaccgc agtcgcggcg ataaccgaag
     2461 tgcgaaacga cgaacgcatt ggcctgcccc ccaaaaatgg caaggacccg cgcgtctacg
     2521 aaactcgccc gaccagtttt ttttcattca gggtcaggca gcccaacgcc gggacccgcg
     2581 cggcaattca agcccgcagg tgcgacacag agcaagcgcg gatcgcgcgg acacttcgcc
     2641 ggtcacacgt tttgtcgcgc cactgtgaca tcgcagccac gaccgccgcg cagattgatg
     2701 cgtgacacgg cgtgacacct tcgatcgacc acgcatctgt aagtttcatg taaggcaagg
     2761 gtacgtttga tactggctca gctttccgat cgacgctctc tgcaagcgag gcggtcagag
     2821 aggagcccgt atcgtggcgc tcatccgccg acagatgtta tatccaagga tccgtggagc
     2881 tcgaaacgtt atcaactgcg gcggcggaac gtcacgttcg gcgcacgatc tcttcgcgtc
     2941 ttacctcatg ggcggaaatt gcgctgcact cgctccgccg agctggtaat taaagccgac
     3001 tgagaacgaa ttaatctgcc aattgctgat gcccacgttg aagggtcgaa cgtcgggttt
     3061 gtcaggaatg agatcgaccc ttccgcttcc gaaattgtag tggttatatt cgaacgtgat
     3121 tgccatattc ggcgcgaacc gatacgcgta gccggcgccg gccgtccatc cgcccaaggt
     3181 tcgcttgctc tcgaaattgg tgccaatttc cggccagaca ctcgtgtctt tgtactgcgc
     3241 ccatgctgcg ccgcccttca cgtacaaaag cgtgttcgcg ccagggcccg gcgtaaatcc
     3301 cacgcggccc acagcggatt ctacgccggg aatccgggtt gcgaagttcc agttacgggc
     3361 tgtgaagtca ccgctgatcg tggagaggcc cccactgccc tcaattccca gcacgacgtt
     3421 gccgatttgc caattgtaac ccaccgccac gctgaagatg gccccgctca ggctggtcct
     3481 gccgaggcca acgccgtcgg gatcgttcca aggattgccg ccaaagcctc cgccaacata
     3541 ggcgccgaag tagacgccgc tccaatcggg aaccactggt tgggccgcga cgccattccg
     3601 cgccatggcc gggtcggttg caatcgcact gccccaggcg atcgcgccga ccatgagtgt
     3661 ggcgcgaagc aaggacgcgc ggatgacatg tttgcggatc attgtcgccc ccgttttttg
     3721 ttcctcgact atatcgccga ccgtcacgat gagtcgcggc ttgcgggggc ggtgcgtgaa
     3781 cattttggtc tgctgtgatt ggagtaccac acgctgagtt atgccttgag cgaacattga
     3841 ttttcctgcg ggaagattct ttgcacaggt atcttgcacc atgccgatat cgcggcaagt
     3901 tcccgatatt tttcaagaat cctcggcccg ccgttccctt tcagaaatct gttgctgtca
     3961 aagcacatca cgcggcaaac ggacatcaat aaaggccgtt caaggtccgc tcacggcagg
     4021 cgcccaataa ccggcgccga agaagccgat ggtcgggagc tttgcgggct gctgctgcgc
     4081 gcgagccgcg agcgggccat accgccgccg cgtcgcttag cagggtggaa tttcagtccc
     4141 tctatgtccg cttcgggtca aagcggacct catcccgtga gggtcacgtc gcattttccc
     4201 cctcggcaat ggtcttctcg agatcggctc gcgaccgggg cgtctgcggc cgctttgatc
     4261 cagtcattgc cgcacgtcgg gcgctgcagc aggttttcgg ttctgctaca atctaaaatc
     4321 gcaaagtccc agggccccct catgcacaaa ctttcattcg gcgacgcgtt ctacgcaggt
     4381 cgcctggtta agttgctgac attcggtggc ctcgctgtcc tggtggcgct tgaatgcctc
     4441 gacgcaactg cccgggcgca aactgccgag aggagaacag atcccgccat tcggccggag
     4501 ttccgagagc ctgtaacgct ggcgagcaag gaaggcgtcc tcgaagttcg cctcaccgcg
     4561 cggcagggcc aggccacgct cgatacggtc gccacgccgg tccagaactt tctcatgttc
     4621 gactatgaag tgattcgcgg cacggcgtcc gacgggcgca gctcgggcgg caatctctat
     4681 ccggcgccca ccctgcacgt atttcccggc gaaacgctga tcgtccattt cgagaacggg
     4741 ctcactggat tgacaatccg ggattacttc attccgcagt acacgccaaa gggtcagccc
     4801 attgcgctct acccgcagca gatgacctcc tcgccgatca atctccacac ccacggagct
     4861 catatcagtc cgaggggaaa cgccgacaat gtgttgctgc acatccctcc aggcatgtcc
     4921 aacacctaca cctacgacct tccaaggaac atgccgcagg gactgtattg gtatcacagt
     4981 catcttcatg ggctcacttc ggcccacgtc tataccggtc tcgtcggact gctggcgatc
     5041 ggacgaacgg acggcaacct gccgctcgtc acggagaaga acattcccat ccgggacatg
     5101 gcgcttcagt acaacttcgt ctttgaccgt gctggcggtc tggcgcaact caacaatgtg
     5161 agctggtccc agtgggtcag cacgataacc ccgcccaagc ccgaggagct cgcgaacgga
     5221 acctatcagc catcgctcgc gccggttaac ttcaagcaat cgaagccggg aacgaagtac
     5281 tttacggtct ggtatgcggg gccgctttca atccacaatc gtcgcgggac cttccagttc
     5341 atccccagca atctgcagca cttcgtcgcg gcggacggca aagccgagag cgatgtggcg
     5401 gcggatgcat cgctgccgga ttaccagcgg gacgtgcaat tcacggtcaa cggccagttt
     5461 cagccggtca tcaagagcaa ggccggacag accgagatct gggtgctcgc gaatgtgagc
     5521 gactttgcct acatgaacgt ccagctcacg gaaaccgcaa cggggcggca tccgccgatc
     5581 gccatcgt
//
LOCUS       DATLJT010000551         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_951265,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000551 DATLJT010000000
VERSION     DATLJT010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951265"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VM910_12715"
     CDS             <1..335
                     /locus_tag="VM910_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63429.1"
                     /translation="IGTHTHMRKTKVSRAVALLEKRKLLARRINRADMRESFLSLTAP
                     GRAIYEELVPHAVDFARRLTDILTPRDRDAFQRALQQITDRSAQLVAEAVEQTSAKAN
                     TSGNARDM"
     gene            332..967
                     /gene="maiA"
                     /locus_tag="VM910_12720"
     CDS             332..967
                     /gene="maiA"
                     /locus_tag="VM910_12720"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVI63430.1"
                     /translation="MKLYSFFRSSAAYRVRIALNLKGISYDTASVHLVKDGGHNKSPE
                     FRAVNPQMRVPVLVTPRGDVLIQSLAIIEYLDETHPQPPLLPKDAVARARVRGLAELI
                     ACDIHPLNNTSPLRYLKNTMGQEQSAIDAWYHHWVITGFEALEALITPGPYACGSEVT
                     LADVCLVPQVNNARRLKVPLERFPKIVGVDRACLALSAFDRARPENQPDAE"
     gene            complement(1240..>1525)
                     /locus_tag="VM910_12725"
     CDS             complement(1240..>1525)
                     /locus_tag="VM910_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63431.1"
                     /translation="LELFEIADHAVEIAAHLLDLRGDRPALRGERREKCEESLAGAAT
                     FVGLCNGAIEVGLLFGNGVLGALDLFGAHRVDGALGQGGKLAFKPYANRI"
BASE COUNT          304 a          499 c          438 g          284 t
ORIGIN      
        1 cgatcggcac gcacacccac atgcgtaaga ccaaggtgtc gcgcgcggtc gcgcttctgg
       61 agaagcgcaa gctcctcgcc cggcgcatca accgcgccga catgcgggaa tcttttctgt
      121 cgctgacagc cccggggcga gccatctacg aagagctggt gccgcacgca gtcgattttg
      181 cccgccgcct gaccgatatt ttgacgcccc gcgaccgcga cgcgttccag cgggctttgc
      241 agcagatcac cgatcgatcg gcgcagctcg ttgccgaagc ggtcgagcag accagcgcca
      301 aagcaaacac gagcggcaac gcgagggaca tgtgaaactc tattcctttt tccgttcttc
      361 agcggcgtac cgcgtgcgca ttgcgcttaa tctgaaaggc atcagctacg acacggcttc
      421 ggttcatctg gtcaaggatg gcggccataa caagtcgccg gagtttcgcg cggtcaatcc
      481 gcagatgcgc gtgccggtcc tggtcacgcc gcgcggcgac gttctgatcc agtcgcttgc
      541 catcatcgaa tatctcgacg aaacgcatcc tcagcccccg cttttgccaa aggacgcagt
      601 cgcgcgtgcc cgcgtgcgcg gtctcgccga gcttatcgcc tgcgatattc acccgctgaa
      661 caatacgagt ccgctgcgtt atctgaaaaa cacgatgggc caggagcaaa gcgcgatcga
      721 cgcctggtac catcattggg tgatcaccgg ctttgaagcg ctggaagcgc tgatcacgcc
      781 cggtccctat gcctgcggta gcgaggtcac gctggccgac gtctgtctgg tgccgcaggt
      841 caataatgcc aggcggctca aagtgccgct cgagcggttt ccgaaaatag ttggcgtcga
      901 tcgcgcttgt ctggcgctgt ctgccttcga tcgagcgcgt ccggagaacc agcccgacgc
      961 cgaatgatat gatgagacat catccacact ttagtcacca ttgcagcgct tgatccgcgc
     1021 gcctaacggg ggtttggggc gagaatgatt cacggaagga gcggcttcgt taaagcgggg
     1081 ctgaaggtcc gacgacaagc aattctgttt gccgccgtga tcatttgttt gcccgtcaca
     1141 gcaaacgcgc agggcatttc cgcaccgccc ggacttgccg cgccagcgcc gccgcgccaa
     1201 ccgatttcga atccgggcgc ggccaatccg gctcccaact caaatcctgt ttgcgtacgg
     1261 cttgaaggcc agcttaccgc cctgacccaa ggctccgtcg acccggtgcg cgccgaacag
     1321 atcaagcgca ccgaggacgc cattgccaaa cagcaagccg acctcgatcg caccgttgca
     1381 caagcccaca aaggtggctg cgccggccag ggattcttcg cacttttctc ggcgctctcc
     1441 ccgcaatgcg ggccgatcac ctcgcagatc cagcagatgc gcggcaatct cgaccgcatg
     1501 atcagcgatc tcgaacagct caaga
//
LOCUS       DATLJT010000552         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1641252,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000552 DATLJT010000000
VERSION     DATLJT010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641252"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VM910_12730"
     CDS             complement(<1..1058)
                     /locus_tag="VM910_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI63432.1"
                     /translation="MQYHFSDHLLDTDRRELRRGEDRIAIEPQVFDLLVYLVENRDRV
                     VRKDDLIANIWDGRIVSESTLTSRINAARNAVADNGKDQKLIRTIQRRGFRFVGVVQA
                     HEAGTQSLDSSSQSLSPAEDRAQDTSPAKLSVLDRPAIAVLPFTNMSGEPEQEYFSEG
                     ISEDIITALSKLRWFYVIARNSSFSYKGRSVHLKQIGDELGVGYVVEGSVRKEGERVR
                     ITAQLNDVVTGSHLWAERYDRDLADVFVVQDEITQAIVAAIEPQLYAAEDCRARRKAP
                     DNMDAWDLVMRALSHYWRVTRQDNVAAQALLEKAISIDPNYGQALGVLAASHTLGAHM
                     GWEEMAAAMPVAERAALAA"
     gene            1219..2079
                     /locus_tag="VM910_12735"
     CDS             1219..2079
                     /locus_tag="VM910_12735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI63433.1"
                     /translation="MTTVTQITPKQSEQPTTSATPDLASLKIRQQAAWSSGNYAVVGT
                     TLQIVGEELCEALDLRSASKVLDVAAGNGMITLAAARRWCDVTSTDYVPALLEAGRRR
                     AAAEGLSIDFIEADAENLPFDNDSFDTVVSTFGVMFTPNQDRAAAELLRVLKRRGHIG
                     LANWTPEGFIGQVFKTLGKYLPPPTGARSPALWGTRARLTELFGSEASSIKAEPRTFN
                     FRYRSPEHFLDVFKTFYGPVLKAFAALAPTKREELQDDLHALIGRMNRAGDGTMIVPS
                     EYLEVVITKR"
     gene            2280..>2539
                     /locus_tag="VM910_12740"
     CDS             2280..>2539
                     /locus_tag="VM910_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63434.1"
                     /translation="MTNPIQLAGAQCTTSTVIALHCSLGSGRQWAHLAETLGQSHRFL
                     APDIAGYGNDPGPFEMPTTLAEEVEALTYGLAEATGPIHLVG"
BASE COUNT          535 a          783 c          725 g          496 t
ORIGIN      
        1 gcggcgagtg cggctcgctc ggctaccggc atagccgcgg ccatctcctc ccaacccata
       61 tgagcgccaa gggtgtggct agccgcaaga acgcccagag cctggccata gttcggatcg
      121 atgctgattg ctttctccag aagagcttga gcagctacgt tgtcctgccg cgtgacgcgc
      181 caatagtggg aaagcgcacg catgacgagg tcccatgcgt ccatattgtc tggtgccttg
      241 cgacgagcgc ggcagtcctc tgcagcgtag agctggggct cgattgcggc gacgatcgcc
      301 tgcgtgattt catcttgcac cacgaatacg tcagcaaggt cgcggtcgta gcgctccgcc
      361 cacagatggc tgcccgtcac gacgtcgttc agctgggcgg ttatacgtac gcgctcgccc
      421 tccttgcgca cactaccttc gacgacgtaa ccaacgccga gttcgtcacc gatttgtttc
      481 aggtgaacag atctgccctt gtaagaaaag gaagaattgc gggcgatgac ataaaaccag
      541 cgcagcttcg aaagcgctgt gatgatgtcc tcgctgatgc cttccgaaaa atactcttgc
      601 tcaggttctc cgctcatgtt ggtgaacggc agtacggcga ttgccggccg atcaagcact
      661 gacaactttg ccggcgacgt atcttgcgcg cgatcttcgg cagggctcag cgactgcgac
      721 gaactatcca gcgattgggt gccagcttcg tgcgcctgaa ccacgccgac aaaacgaaat
      781 cctcttcgct gaatcgtgcg gatcaatttc tggtccttgc cattatcggc gacggcgttg
      841 cgagcggcgt tgattctgct cgtcaacgtc gattctgaga cgatccggcc atcccagatg
      901 ttggcgatca aatcatcctt acggacgacg cgatcgcggt tctccacgag atagaccaac
      961 aggtcgaaca cctgcggttc gatggcaatc ctatcttcgc ctcggcgtag ctcgcggcgg
     1021 tctgtatcga gcaaatgatc tgaaaagtga tactgcacct acgcacaccc cgctcgtggg
     1081 cctttctgaa ggaaaatcag gctgaactca aggaccagac aagcagtcgt cagagtcttt
     1141 ctgtgcgggt tcggccactt tcggttcatc gtcagtcacg tcccgtgacc gcataacgac
     1201 actgaaggcc agacaacaat gaccactgtg acgcaaatca ccccaaaaca gagcgagcaa
     1261 cccaccacgt cggcgacacc agaccttgcc tcactcaaga tacggcagca ggcggcctgg
     1321 tcgtcgggca actatgccgt tgtcggcacc acgctgcaga tcgtcggcga ggaactttgc
     1381 gaggcgctcg atctcagatc agcttcgaaa gtgctcgacg tggccgcagg caacggcatg
     1441 atcacgctcg ctgcggctcg tcgctggtgc gatgtcacct cgactgacta tgtgcccgcg
     1501 ctgctggaag cggggcgtag gcgcgccgcg gcggagggcc tgtcgatcga cttcatagag
     1561 gctgacgctg agaacttgcc gttcgacaac gatagcttcg atactgttgt ttccactttc
     1621 ggcgtgatgt tcacgccgaa ccaggacagg gcagccgccg agttattgcg ggtcttgaag
     1681 cgcaggggac acatcgggct cgcgaactgg acgcccgaag gcttcatcgg ccaggtcttc
     1741 aagacactcg gcaaatacct gccgccgccg accggcgcca ggtcacccgc actgtgggga
     1801 acgcgcgcac gtctcaccga gctgtttggc tcggaggcaa gctcgatcaa ggcggagcct
     1861 cgcaccttca acttccgcta tcgttcgcca gagcattttc tcgatgtgtt caaaactttc
     1921 tatggcccag tcctgaaggc gtttgcggcg ctcgcgccga cgaagcggga agagcttcaa
     1981 gacgatctgc acgctctaat cgggcgcatg aacagagctg gcgacggcac catgatcgtg
     2041 ccgagtgaat acctcgaggt cgtgatcacc aagcggtgac atgagcccga atcgccggct
     2101 ccgcggactg atccggagtt ggtcacgacg ccaagccgtc gcccacgagt gccgcgcgat
     2161 caatcgaacg attggcagga tggaagaccg gtaattgcgg atctcggact catccgccag
     2221 ccgaagcgag caaccgacga caaccttgca accgcatcgg cctaaatagg agcgatccca
     2281 tgactaaccc aatccaactt gctggcgcgc aatgcacgac aagcaccgta atcgccctgc
     2341 actgctcgct cggttcaggt cggcaatggg cacatctcgc cgaaaccctc ggtcaaagcc
     2401 atcggtttct cgctcccgac atcgccggct atggcaacga tcctgggccg tttgagatgc
     2461 caacaactct ggcggaagag gtcgaggcct tgacctacgg ccttgccgaa gcgaccggac
     2521 cgatccacct tgtcggcca
//
LOCUS       DATLJT010000553        11997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2790585,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000553 DATLJT010000000
VERSION     DATLJT010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790585"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VM910_12745"
     CDS             complement(<1..351)
                     /locus_tag="VM910_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63435.1"
                     /translation="MTAKMKRRDFITLLGGAAAAWPLAARAQQRERMRRIGVLMSLAA
                     DDQEGQARLTAFVQGLQELGWTDGRNVQIDYRWPAGDAERIRRYAAELAALAPDIILA
                     GGGAVVPSLLQATRA"
     gene            complement(348..1340)
                     /locus_tag="VM910_12750"
     CDS             complement(348..1340)
                     /locus_tag="VM910_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63436.1"
                     /translation="MQFDQLRRCAFITILGGAAAWPIAAGAQQSALPVIGFLNSRGPS
                     EDAYILAAFRQGLKETGYVEGQNVVVEYRFAQNRYDRLPVLATDLVRRQVAVIVVNTP
                     AALPAKAATTTIPIVFLTSVDPVEMKLIASLNRPGGNLTGVTVLGVEIGPKQLQLLHE
                     VIPAATTMAVLINPANPAIAEAQSRDMHAAARTLGLAAHVLHASTERDFDMVFATLVQ
                     LRVGALAIGSDPFFTSRTEQLAALALRHAVPAIFQFREFAAAGGLMSYGSSIRDTYRL
                     GGIYAGRILKGENPADLPVQQSTKVELVINMKTAKALGLTVPITLLARADEVIE"
     gene            complement(1447..2040)
                     /locus_tag="VM910_12755"
     CDS             complement(1447..2040)
                     /locus_tag="VM910_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI63437.1"
                     /translation="MAKVQVRQTIKPRERLLDAAYDLFATNGISQVGIDTILAKSGCA
                     KASLYSNFQSKVDLAVAFLDRREAVWTRGWLEAEIKRRASAPEGRLLAIFDVFDGWFR
                     KRTFEGCSFINVLLESKANSPVHRAAAAHLSKIRAIVRALANDANLREPEKFAQAWHM
                     LMKGAIVSACEGNRNAAREAKCAAQLIIDGWPRKRRR"
     gene            2360..3514
                     /locus_tag="VM910_12760"
     CDS             2360..3514
                     /locus_tag="VM910_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63438.1"
                     /translation="MPIPTEPVGSLPRPAKLQAAYADYDAGKITKEQLTQEQDAACLD
                     SIKRMEATGSPIVSDGEQRMSSFATYPLTDTLAGTGLADHLAGDGQYFAIFADGHHRQ
                     LPRLTGGPFRYKTYAAEFTEKAMKMTTKPLKQAVIAPSMLALLYPLDGEVKGCSREQF
                     LSDLCDECEKDIRGAFKAGAARVSIDFTEGRLACRNDPRNPWTGRKMLAGFIDLNNRV
                     INRFSAEERKNIGIHTCPGGDCDSTHSADVDYADLLPSMFKMNAGYFLIQLSSEKDKE
                     RVYKLIGQYSRPDADGIPQVCFIGVTNPQNPRVETPQEVCDDLLLASKHIPKERLGST
                     DDCGFSPFSIDVKPKHGSPDVARDIAFQKIRSRVEGTKMASEKLGVARAA"
     gene            complement(4074..5078)
                     /locus_tag="VM910_12765"
     CDS             complement(4074..5078)
                     /locus_tag="VM910_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63439.1"
                     /translation="MFAGLKIWILVAAAAAGPIIESSVASAQNPQPVSVIAFPGTGNW
                     PIRIGQDKGYFAQAGIEVTLTPTPNSVFQMTNLIEGRFDIAMTAVDNVIAYMEGQGEV
                     AVSRQPDLFVFMGGSPSMPALTTIPEVEGYQELKGKALAVDAVTTGYAFVLFDLLKRN
                     GLQLSDYKVEPVGGTPARWQGLRERKYAAAILTSPFDLIARSNGFHVMQYAKDVYGHY
                     EESVAATRRSWATTNEQKLIAYIKGYAAAVEWLRDSKNEDEAISILRKYFPQLSPELA
                     TATYANFVGSRGIAAKAQLDIAGVRKVLELRSEYGRPKKALTDPTRYYDPRYYEAAIR
                     "
     gene            complement(5090..5998)
                     /locus_tag="VM910_12770"
     CDS             complement(5090..5998)
                     /locus_tag="VM910_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI63440.1"
                     /translation="MLLYGLCTSAAIGQAEQPDQSRSPIATRLSINMRQAKPLSQAEE
                     KALRRLDIFKECEACPEMIMIPAGEFMMGAPDDEEDSEDNERPRHKVTIPKPLAVGRF
                     AVTFDEWDTCVADRGCRGYRPSDRGWGRGRRPVLNLWWDDARAYVKWLSDKTGKPYRL
                     LTEAEREYATRAGTTTPFWWGSSLSTDRANYDGSFTYPFVAGLKGEYRRKTLPVDSFA
                     PNPWGLYQVHGNIYEWVEDCWHRNYEGAPTDGSAWDTPDCTRHVLRGGAWNFASWHLR
                     SASRGSVASAVELLPVGMRVARTLAR"
     gene            complement(6178..7176)
                     /locus_tag="VM910_12775"
     CDS             complement(6178..7176)
                     /locus_tag="VM910_12775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI63441.1"
                     /translation="MSSAGRTFAQAPSVRSVSGGRIDVHHHFLPPQYMKEEHERLNFA
                     HGSVSASQMLSWTPSQSLEVMDQNGIATAVVSITTPGVWFGDVAAGRRLSRMWNDYAA
                     EQIRDHPGRYGLFAVAPLPDIDGSLREIEYAVDKLKADGIGFLSSYDGKYLGDAAFAP
                     VFAELDRRHAVVYVHPTVSACCGNVIPGLPPQAIEYPLETTRTITSLLINGIPVKHPN
                     VRWIFSHGGGATPMLAGRMAETVARRPNSAEVMPNGVLAELRKLYYDTASAASPASMA
                     ALRIMAPASHILFGSDYPFAKAAAGIEELRHTPMSDAEREAIDRGNAMVLLPRLKP"
     gene            complement(7443..7949)
                     /locus_tag="VM910_12780"
     CDS             complement(7443..7949)
                     /locus_tag="VM910_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI63442.1"
                     /translation="MGIHLGRVGHIGIHVSDVDRSIDFYRRVLGLKLTGRWGPPDIGR
                     PICFMRIDDKHHDVVLFELPEDARTAGVAVTDSSLRRAPGLDHIAFEVNSREDWLLAL
                     DHVRSCGVEIVSGPYVHAPEGGEKGFVGGSGSHAFYFLDPDGNRIEVYCWMMKVTGPS
                     LAAPHPDL"
     gene            complement(8129..8755)
                     /locus_tag="VM910_12785"
     CDS             complement(8129..8755)
                     /locus_tag="VM910_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63443.1"
                     /translation="MSALLHLLSLSRADNGISARDMAMFDLDQFIADCRAALAADKSH
                     KNVREIVARAVSDPAAILKGLGEPKRSEVQKLYHSGDLTILNVIWAPRMTIMPHNHQM
                     WAVIGIYTGREDNIFWRRVPGGHSGKLEAVGARALSAKDADPLGDNIIHSVTNPIPRL
                     TGAIHVYGGDFFGAERSEWDPETLQEGRYDAAKTMRLFEEANALYRTG"
     gene            complement(8757..11750)
                     /locus_tag="VM910_12790"
     CDS             complement(8757..11750)
                     /locus_tag="VM910_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI63444.1"
                     /translation="MSLKGAKSPTRGRKLRSTGTKAGTRVASSSESQAALIKKLKSHA
                     RDLEKKLETRTHDLAEALEQQTATSEVLRVIASSPGDAQPVFDTIAKGVERLCKAQFC
                     HVFRFDGELIHFAAHYGYGPEAAETIRRGYPIAPGRASAAARSILSGAVEEIPDVDAD
                     ADYAHGTIVKAINVRSIVAVPMLKDGCPIGAIVMARTQSGPFPAPLIKLIQTFADQAV
                     IAIENTRLLNELRESLQQQTATSEVLQVISGAPGELDPVFQAVLENATRLCDAKFGTL
                     NLYHGEILRNVALYNVPSAYLETKLHEVIRPHPKSGHAHIVRTKQVVHIEDLTAMPPY
                     REGDPAVAALADLGGARTIVVVPMLKQDELIGTIGIYRQEVRPFTQKQIELVSNFAKQ
                     AVIAIENMRLLNELRESLQQQTATADVLKVISRSAFDLQSVLDTLVESAARLCEAERG
                     SIWRPRGTIYHLAASYGHSEEWKSSMRQEELEAGRGSITGRVRLGATTVHVHDIQADP
                     EFAVRTSIAHGTRTMLGVPLLREGTPIGVIVMSRNVVRPFTDKQLELVTTFADQAVIA
                     IENVRLFDEVQARTRELSESLQQQTATADVLKVISRSTFDLKSVLQTLVESAGRRCDA
                     DYATITRQKDGVLFFAEAYGYSSEFIEYVKAMPVERGRGTATGRALLEGRVIHIADVL
                     ADPDYTWAEAQRLGGYRTVLGVPMLREGIPVGVLTLTRSEVRPFTEKQIELVQTFADQ
                     AAIAIENVRLFDEIQDKNRQLAEASEHKSQFLASMSHELRTPLNAIIGLTEMMVANAA
                     RFGTEKALEPLRRVNAAGTHLLSLINEILDLSKIEAGKLELNPEPVNLARLIDEVIGT
                     AGQLAEKNKNRLVVEAQENVGALTADPIRLKQILLNLLSNACKFTKEGEVTLRVRKVA
                     DGREWVELAVADTGIGMTAEQQAKLFQDFTQADSLTSRRYGGTGLGLAISRKLARMMG
                     GDVTVTSEPGKGSVFTVRLPGGANT"
BASE COUNT         2353 a         3717 c         3554 g         2373 t
ORIGIN      
        1 cgcacgggtc gcctgtagca acgatggtac gacggcgccg ccaccggcca ggattatgtc
       61 cggcgcgagc gcggccaatt ccgccgcgta tctgcgaatg cgctcggcat cgcctgcggg
      121 ccagcgatag tcgatctgca cgttgcggcc gtcggtccag cccaattcct gcagaccttg
      181 cacgaacgcc gtcaggcgtg cctgtccttc ctgatcgtct gcggcgaggg acatgagcac
      241 gccgatccgc cgcatccgct cgcgttgctg cgcccgcgcc gcgagcggcc acgcagcggc
      301 cgcgccgccg agaagcgtga tgaaatcgcg ccgcttcatc ttcgcggtca ttcgatcacc
      361 tcgtcggcac gggcgaggag cgtgatcggg actgtgagac cgagcgcctt ggcggtcttc
      421 atgttgatga cgagttcgac tttcgtagat tgctgcaccg gcaggtccgc cgggttctcg
      481 cccttgagga tgcggccggc gtagatgcca ccgagacgat acgtgtccct gatactgctt
      541 ccgtagctca tcaggccacc ggccgctgcg aattctcgaa actggaagat cgcaggtacc
      601 gcgtggcgga gcgccagcgc ggcgagctgt tcggtgcggc tggtgaaaaa gggatcggag
      661 ccgatcgcga gcgcgcctac gcgcagttga accaaggtag cgaagaccat gtcgaagtcg
      721 cgttcggtgc tggcatgcag gacatgggca gccagcccga gcgtgcgggc cgccgcgtgc
      781 atgtctctcg attgtgcctc cgcaatggcc ggattggccg gattgatcag cacggccatg
      841 gtggtcgccg cagggatcac ttcatgcagc agctgcagct gcttcggccc aatttccacg
      901 cccagcacgg ttacgcccgt aaggttgccg cccggtcggt tgaggctggc gataagtttc
      961 atctcgaccg ggtcgacgct tgtcaaaaag acgatcggaa tcgtagtggt cgccgccttt
     1021 gcgggcagcg ccgcaggagt attcacgacg atcacggcca cctgccggcg aaccaaatcg
     1081 gtcgccaaca ccgggagtcg gtcgtaccga ttttgcgcga aacggtattc aacaacgacg
     1141 ttttggccct cgacatagcc cgtttctttc aggccctggc gaaacgccgc caagatatat
     1201 gcatcctcgc tgggccccct gctgttgaga aatccgatca ccggcagcgc cgactgctgc
     1261 gcgcccgccg cgattggcca cgccgccgcg ccgccgagaa tggtgatgaa cgcgcagcgt
     1321 ctcaattgat cgaattgcat tcttcccgcc agctaggtcc tagatcgtag gatatcgaat
     1381 tggcgaggat cagtcaggag gtaacggaat gattttacaa caccgaacgg tgacgtagtt
     1441 cgccgattag cgccgtcgtt ttcgcggcca accatcaata atgagctgag cagcgcactt
     1501 cgcctctcgc gccgcattcc gattgccttc gcaggccgaa acgatcgcgc ccttcatcag
     1561 catatgccag gcctgtgcaa atttttctgg ctcgcgcaga ttcgcatcgt tggccaacgc
     1621 gcggacaatc gcccgaatct tggataaatg ggccgcagcc gcgcggtgaa caggactatt
     1681 cgctttcgat tcgagcaaca cgttgatgaa tgagcatccc tcaaaagttc tcttacgaaa
     1741 ccagccgtca aaaacgtcga aaatggccaa gagccgccct tctggcgccg atgcgcggcg
     1801 cttgatctcc gcttccagcc acccgcgcgt ccacacggct tcacgccggt ctaggaaagc
     1861 aacggccaga tcaaccttgg actggaaatt gctgtaaaga ctcgcctttg cacaacccga
     1921 tttggcgaga atcgtgtcga taccaacctg gctgatcccg ttagtcgcaa acaaatcata
     1981 agccgcgtcg agaagtcgct cgcggggctt gatcgtttgc cgaacttgaa cttttgccaa
     2041 aatcccggtc ctcaatagca gtgagacatg gaaccatccg tgagtgcaat tcgtatcatc
     2101 ctgtccgcac gccacgcaaa cgtgcccact cccttttgac gccccgcgtt ttcgaccagt
     2161 ccgtctcgtc ccgaagaatc gtctcccgac ccgcgcggta atgtagcccc gtttggcggt
     2221 gccaccttgg cgcagcggcc tctcgcagag ggcgccgttc tggattttac ttgacctcat
     2281 tcctgaatat agacagactg gtctgttcgt gccagcccgt atgtctgcca agaaaaatat
     2341 cgatacacag gaggaacgca tgccgatccc aaccgaaccc gtcggatcgc ttccacggcc
     2401 agccaagctt caggccgcct atgccgatta tgacgccggc aagatcacaa aagagcagtt
     2461 gacgcaggag caggacgccg cctgcctcga ctcgatcaag cgcatggagg caacgggctc
     2521 tccgatcgtc tccgacggcg agcaacgcat gtcaagcttc gctacctatc ccctgactga
     2581 cacgctcgcc ggaaccggcc tcgccgatca ccttgcaggc gacgggcagt atttcgccat
     2641 cttcgccgat ggccatcatc ggcaattgcc gcgtcttacg ggagggccat tccgatacaa
     2701 gacctacgca gccgagttca ccgagaaagc gatgaagatg acgacgaagc cgctcaagca
     2761 ggcggtcatt gcgccatcga tgctcgccct gctctatcct ctcgatggcg aggtgaaggg
     2821 ctgctcgcgg gaacaattcc tcagcgatct ctgcgacgaa tgcgaaaagg acattcgcgg
     2881 cgctttcaag gctggtgcag cccgcgtttc gatcgacttc acggaggggc ggctcgcgtg
     2941 caggaacgat ccgaggaatc cgtggaccgg acgtaagatg cttgcgggtt tcatcgacct
     3001 caacaaccgg gtgatcaatc ggttctcggc cgaagagcgg aaaaacattg gcatccacac
     3061 ttgccccggg ggcgactgcg actcgacgca tagcgccgat gtcgattacg ccgacctgct
     3121 gcccagtatg ttcaagatga acgcaggcta cttcctgatc caactctcaa gcgaaaagga
     3181 caaagagcgc gtctacaagc ttatcggtca atacagcagg ccggatgcgg acggcatccc
     3241 gcaggtctgc ttcatcggcg taaccaaccc acagaacccg cgggtcgaga cgccgcaaga
     3301 agtttgcgat gatcttctgc tcgcctcgaa gcacatccca aaagagcggc tcggctcgac
     3361 cgacgactgt ggtttctcgc cattcagcat cgacgtgaag ccgaaacacg ggtcacctga
     3421 cgtggcgcgg gatatcgcct tccaaaaaat ccgatcccgg gtagaaggca ccaaaatggc
     3481 atccgagaag ctgggcgtcg ccagggccgc atagtcggag atggcagtgg ctgaggctgt
     3541 ctggagcacg cgggcagccg cgaagggggt gacggggaaa tcccatgtca cggcgggatg
     3601 gacggcgacc ggccatcctc ggcaaccttg cacgtcgtcc gaaccgccgt gagtcgcgtc
     3661 cgacctttcc agaccgacct acggcgcccg tccgttcaac gaaaacgacc ggcgggcgac
     3721 ccgacaggaa ctcgctggtg ggcatctggc gggcgggcga tcatctccag ccttctaaac
     3781 tgccggcgcc tgtcccgtcg acagagcgct gacccggtcg gccgcaagcg gcttaggtat
     3841 ctgaagccgg gtcacctgtg cctcattcgc tttcgtccca tccatcaatc ttgagttcaa
     3901 gacgtcagac gagttgaccc gaacaaagtg aggagcagtc gaaggggtac ctctggatcc
     3961 accagcacca accggaccag ctttggctcg ggcgtttact catgaatcgc gctgggtagg
     4021 cggacgtcgc gacaggtccg agaagtgctg ccattagctg acgagccagt gccctaacgg
     4081 atggctgcct cgtaatatcg ggggtcatag tagcgagtag gatcagtgag ggctttcttc
     4141 ggtcggccgt attcgctgcg cagctccagc accttgcgaa cgcccgcgat atcaagttgc
     4201 gcttttgctg cgataccccg agagccaacg aaattcgcgt aggtcgcagt tgccaactct
     4261 ggtgacagtt gtggaaagta cttgcgcaag atcgaaattg cttcatcctc attcttcgaa
     4321 tcgcggagcc attcgaccgc tgcggcgtac cctttgatat atgctatgag tttctgctcg
     4381 ttggtcgtag cccatgatcg ccgtgtggcc gctaccgact cctcgtagtg gccgtaaacg
     4441 tcctttgcgt attgcatgac atggaagcca ttggacctgg caatcaaatc aaacggcgac
     4501 gttaggatcg cagcagcata cttacgttcg cgcaagcctt gccaacgcgc tggagtgccg
     4561 ccgactggtt cgaccttgta gtcgctcaac tgcaatccat tgcgcttgag aaggtcgaac
     4621 agtacgaacg catatccggt cgtcacggca tccactgcaa gcgctttgcc tttaagttcc
     4681 tgatacccct cgacttcagg gattgtggtc agggcaggca tgctcggact gccgcccatg
     4741 aatacgaaaa gatcgggctg cctcgaaaca gccacttctc cttggccctc catatacgcg
     4801 atgacgttgt cgaccgcggt catcgcaata tcgaacctgc cttcgatgag gttcgtcatc
     4861 tggaacaccg agtttggcgt tggtgtcagc gtcacctcga tgccggcttg tgcgaagtag
     4921 cctttgtcct ggccaatcct gatcggccag tttccggtgc cgggaaatgc gatcacagag
     4981 accggctggg ggttctgggc gctcgccacc gatgactcga tgattgggcc ggcagctgct
     5041 gctgccacta agatccagat tttaagtcca gcaaacatca ccgatctact caacgcgcta
     5101 gtgttctggc gacgcgcatt ccaaccggca gaagctcgac tgcagacgcc accgaaccgc
     5161 gtgaagcaga gcgaaggtgc catgatgcaa aattccatgc gccgccgcga agcacgtggc
     5221 gagtacagtc gggcgtgtcc catgctgatc catcagtggg cgcgccttcg taattgcgat
     5281 gccaacaatc ctccacccat tcatagatgt tgccgtggac ctgataaaga ccccagggat
     5341 tgggggcgaa tgagtcgaca ggcagtgtct ttcggcgata ctcgcccttc aacccagcga
     5401 caaacggata ggtaaagctg ccgtcgtaat tcgctcggtc ggttgaaagc gacgaacccc
     5461 accaaaatgg cgtcgtcgta ccagcccgtg tcgcatattc ccgttccgcc tcggtcagga
     5521 gccgataggg cttgcctgtt ttatccgaaa gccacttcac atacgcccga gcatcatccc
     5581 accaaagatt gaggaccggc cgccgtccac ggccccatcc tctatcggat ggtcgatagc
     5641 cccgacaccc acggtcggcc acacacgtgt cccactcatc aaatgtcacc gcaaagcggc
     5701 cgactgcgag cggctttgga atcgtcacct tgtgtcgtgg cctttcgttg tcctcgctgt
     5761 cttcttcatc gtccggcgca cccatcatga attcgccggc agggatcatg atcatttccg
     5821 gacaggcctc acattctttg aaaatatcca gtcgtcttag agccttctct tcggcctgcg
     5881 acagcggctt tgcctgacgc atgttgatag aaaggcgggt tgcaatcggg gagcgtgatt
     5941 gatccggctg ttcagcctgg ccaattgctg ccgacgtgca gaggccgtac agcaacatca
     6001 ccaacaaaga tcggccggcc aaacacattc ttagttccgg cttcacacgg cgcctgcagg
     6061 cttccctctt tatcttaagc tcacccacca aagaacacgt acatgcgcca acagctgtca
     6121 gcggtgcgac tgcaaggatc tgcgaccaaa tcatgactat cggccagaca agatggatca
     6181 gggcttcaga cgcggcagca gcaccatagc atttccacga tcgattgcct cccgctcggc
     6241 gtccgacatc ggcgtatgtc gcaattcctc gatgccggcc gcggcctttg cgaaggggta
     6301 gtcggacccg aataggatgt ggcttgctgg cgccatgatt cgcagcgcgg ccatggaggc
     6361 cggcgatgcg gcgctcgcgg tgtcgtaata gagcttgcgc agctccgcca agaccccgtt
     6421 cggcatgacc tcggcggaat taggacggcg agcaacggtt tcggccatgc gccccgccag
     6481 catcggcgtc gcgccgccac catgggagaa gatccagcgg acgttcggat gctttaccgg
     6541 tatgccgttg atgagcaggg aggtgatggt acgcgtggtc tctaacggat actcgatcgc
     6601 ctgcggcggc agaccgggaa tgacgttgcc gcagcaggcc gacaccgtcg ggtgaacata
     6661 gacgacggcg tgacggcgat cgagctccgc gaacacaggc gcgaaggcgg catcgccaag
     6721 atatttgcca tcatacgagc tcaggaagcc gatgccgtcg gccttgagct tatcgaccgc
     6781 atactcgatt tccctgaggc tgccgtcaat atccggcagc ggcgcgaccg cgaacagccc
     6841 gtagcggccg ggatgatcgc gaatctgctc ggcggcatag tcgttccaca tccgcgacag
     6901 tcggcggccg gcggcaacgt cgccgaacca aacgcccggc gtggtgatcg agaccacagc
     6961 agtagcgatg ccattttgat ccatgacttc gagcgactgg ctcggggtcc aggacaacat
     7021 ttggctcgct gaaacactgc cgtgcgcaaa attgagacgt tcatgctctt ccttcatgta
     7081 ctgcggcggc aggaaatggt gatgcacgtc gatccgcccg ccgctcacgg acctgaccga
     7141 cggcgcctgc gcgaacgtgc ggccggccga agacatggcg gccgtcgcag cgagaccggt
     7201 aaggaaccga cggcggccga cgcagcatct gcaaggcgac gccccgtcat cggctgtgaa
     7261 gctgacagac tcacgctcgg ctgagaaggt catcgtagta tccccgggta aggcacgcgg
     7321 ggcgtaatgc tggaagtctc tgcgctgcgc gcccgaagtg tcgtaccgta tgtcggcctc
     7381 ggcgaggccc ccgccaaacg cgccaaagac caagactgag atcgcctctt cccgacgtga
     7441 tcctacagat caggatgcgg ggcggccaag ctcggccccg tcactttcat catccagcag
     7501 tagacctcga tccggttgcc gtccgggtcg aggaagtaaa aggcgtggct gccgctcccg
     7561 ccgacgaagc ccttctcgcc tccctcaggg gcgtgcacgt aggggccgct gacgatttca
     7621 acgccgcacg agcgcacgtg atcgagggcc agcagccagt cctcgcgtga gttgacctcg
     7681 aacgcgatgt ggtcgagacc gggagcccgg cgaagagacg agtcggtcac ggcgacgccc
     7741 gccgtcctcg cgtcctccgg cagttcgaac agcacgacgt cgtggtgctt gtcgtctatg
     7801 cgcatgaagc agatcgggcg cccgatgtcc ggcgggcccc agcgcccagt cagcttgaga
     7861 cccagaaccc tgcggtagaa gtcgatagag cggtcgacgt cgctgacgtg gatgccgatg
     7921 tgcccaaccc ggcccagatg aatgcccatg ctgttctcct aagctgcaat ttgcggtcat
     7981 cgccgatctg gagaccctca ctggtgtcga agttcgccgc gcgcgcgtcg acaagggcta
     8041 taaaaggcgc gctgatggaa tgcttaagca cggctgtcgg caccgcttcc gggtctggat
     8101 ttcaggccag gctccgatcg catccgtatc acccggttcg gtagagtgcg tttgcctctt
     8161 cgaacagcct catggtcttc gcggcgtcgt agcgaccctc ttgtagggtc tcggggtccc
     8221 actcgctgcg ctcggcgccg aaaaagtcgc caccgtagac gtgaatggcg ccggtcagtc
     8281 gggggatcgg attggtcacc gaatggatga tgttgtcacc caggggatcc gcatctttcg
     8341 cggacagggc cctcgcgccc acggcttcca gcttgccgct atggccgccc ggcacccggc
     8401 gccagaaaat attgtcctcg cgacccgtat agatgccgat caccgcccac atttgatgat
     8461 tgtggggcat gatcgtcatt cgcggcgccc aaatcacatt caagatcgtc agatcgcctg
     8521 agtggtagag cttctggacc tcagaccgct ttggttctcc cagtcctttg agaatggcag
     8581 cggggtctga gaccgcgcga gccacgatct cacgtacatt tttgtgcgat ttgtccgccg
     8641 ccaacgcggc gcggcaatcg gcgatgaact ggtccagatc aaacatagcc atatccctcg
     8701 cgctgatacc gttgtccgct ctactcaagg acaataagtg aaggagcgca ctcattctag
     8761 gtgttggcgc cgcccggcag acgcaccgtg aacaccgatc ctttgcccgg ttcgctggtc
     8821 acggtcacgt cgccgcccat catgcgcgcg agcttgcgcg agatcgcaag gccgagcccg
     8881 gtgccgccgt agcgccgcga agtcaggcta tcggcctggg tgaaatcctg gaacagtttg
     8941 gcctgctgct ccgccgtcat gccgatgccg gtatccgcga cggcaagctc aacccactcg
     9001 cgtccgtccg ccaccttgcg cacccgcaaa gtgacctcgc cctctttcgt gaacttgcag
     9061 gcattgctga ggagattgag cagaatctgc ttgagccgga tggggtcggc agtgagcgcg
     9121 ccaacgtttt cttgggcttc gacgacaagg cggttcttgt tcttctcggc aagctgtccc
     9181 gcggtgccga tgacctcgtc gataagccgg gccaaattaa cgggctcggg attaagctcg
     9241 agcttgccag cttcgatttt tgagaggtcg agtatctcgt tgatgaggct caggaggtgg
     9301 gtgccggcgg cattcacacg gcgcagcggc tccagcgcct tttccgtgcc gaagcgcgcc
     9361 gcgttggcga ccatcatctc agtcaggccg atgatggcgt tgagcggcgt gcgcagctcg
     9421 tggctcatgc tggcgaggaa ctgcgacttg tgctcgctcg cctccgccag ctgccggttc
     9481 ttgtcctgga tttcatcgaa cagccgcacg ttctcgatcg cgatcgcagc ctggtcggca
     9541 aaggtttgaa ccagctcgat ctgcttttcg gtgaacgggc gtacttcgga gcgcgtcaac
     9601 gtcagaacgc cgacagggat gccctcgcgc aacatcggca cgccgagcac cgtgcggtag
     9661 ccgcccaacc tctgcgcctc cgcccaggtg tagtccgggt cggccaagac atcggcgata
     9721 tggatcaccc gaccttccag caaggcccgt cccgtcgccg tcccgcgtcc ccgctcgacg
     9781 ggcatagctt tgacatattc gatgaactcc gacgagtaac cgtaagcctc cgcgaagaag
     9841 agcaccccgt ctttctgccg agttatcgtg gcgtagtcgg cgtcacaacg gcggccagcc
     9901 gattcaacaa gcgtttgcag caccgacttg agatcgaagg tcgagcggct gatgaccttg
     9961 agcacgtcgg cggtcgcagt ttgctgctgc agcgactcgc tgagctcgcg cgtgcgcgcc
    10021 tgcacctcgt cgaacagccg cacgttttcg atcgcgatca ccgcctggtc ggcgaaggtg
    10081 gtgaccagct ctaactgctt atcggtgaac ggccgcacga cgttgcgcga catcacgatc
    10141 acgccgatcg gcgttccctc ccgcagcagg ggaacgccca gcatggtgcg cgtgccatgc
    10201 gcgatcgaag tacgaaccgc aaattcggga tcggcttgaa tgtcgtggac gtggacagtc
    10261 gttgcgccga gccggacgcg cccggtaatc gatccccgtc cggcttccag ctcttcctgt
    10321 cgcattgagc tcttccactc ctcggagtgg ccgtagctgg ccgccagatg gtagatcgtg
    10381 ccgcgcggac gccatatgct tccgcgctcc gcctcgcaca gccgcgctgc cgattcgaca
    10441 agtgtatcca aaacgctctg caaatcgaaa gccgaacggc tgatcacctt gagcacgtcg
    10501 gcggtggcgg tctgctgctg cagcgactcg cgcagctcgt tgagcaggcg catgttctcg
    10561 atggcgatga cggcctgctt cgcgaaattg ctgacaagtt caatttgctt ctgcgtaaac
    10621 ggtcgtacct cctggcggta aatgccgatg gtaccaatca gctcgtcctg cttgagcatt
    10681 ggaacgacca cgatcgtgcg agcaccgccc aggtcggcaa gcgcggcgac cgccggatcg
    10741 ccttcgcgat aaggtggcat cgctgttagg tcttcgatgt gtacgacctg cttggtccgg
    10801 acaatgtgag catgaccgga ttttggatga ggacggatca cctcgtgcaa tttggtttcc
    10861 aagtatgccg acggcacatt gtagagcgcg acgtttcgga gtatctcccc gtggtatagg
    10921 ttgagcgtgc cgaacttggc gtcacacagc cgcgtggcgt tctccagcac tgcctggaat
    10981 acgggatcga gctcgccagg cgcgccgctg atgacttgga gcacctctga agtcgcggtc
    11041 tgctgctgta gggattcgcg tagttcgttg agcaggcggg tgttctcgat ggcgatcacc
    11101 gcttggtcgg cgaatgtctg aatgagtttg atcagtggcg cgggaaaggg gccactttgg
    11161 gttcgagcca tgacgatcgc cccgatcgga cagccgtcct tgagcatcgg gaccgccacg
    11221 atgctgcgga cgttgatggc cttgacaatg gtgccatgtg catagtcggc gtccgcgtcg
    11281 acgtccggga tttcctcaac agcgccgctg aggatcgagc gagccgcggc actcgcacgt
    11341 cccggcgcga tgggataacc acgccgaatg gtctcggccg cttcgggccc gtatccgtag
    11401 tgggcggcaa aatggatgag ctcgccgtcg aaccgaaaca cgtggcagaa ttgtgccttg
    11461 cagagtcttt ccacaccctt ggcgatcgta tcgaagaccg gctgggcatc gccgggcgag
    11521 ctcgcgatca cgcgcagcac ctccgacgtt gccgtctgct gctccagcgc ctcggcgaga
    11581 tcatgagtgc gcgtttcgag cttcttttcc agatcgcgag cgtgcgactt gagcttcttt
    11641 atcagtgccg cttgggattc actcgacgaa gcaacgcgtg ttcccgcctt cgttccggtc
    11701 gaacgaagct tgcggccacg cgttgggctc tttgcgcctt tgagagacaa gggtcacctc
    11761 gtcggccagc caaggatgga gcctatcaat cctaacagaa ccgcacgatg aactcgcgcc
    11821 ttttcacact cgatcacctc gtcgccgcta ttcgcggaac ggtgaggttt ctatgaggtc
    11881 gctcggtgga gacgagcctc gaacacgcag atgagcgcga ccttatagaa accttcgttg
    11941 gacgaagccg ctggcactgt cgagcagcga gggagacttt cagctcggac gaatttc
//
LOCUS       DATLJT010000554         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1411321,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000554 DATLJT010000000
VERSION     DATLJT010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411321"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>2244)
                     /locus_tag="VM910_12795"
     CDS             complement(150..>2244)
                     /locus_tag="VM910_12795"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63445.1"
                     /translation="RYANGGMAALGREQGWRPGRRRISGKRLRSIEMLKSQGMSNRAI
                     AHRLGVTEKAIRKLVGPSKAAESAQLVLPAIATEAGKPAASGVPSAAPSGEDGDRATE
                     SAQETVTAPEAVNDDERVPMSLDRDASDRTLDRQLALYGLLEDAAPLFCEGSSVPGAG
                     VLLALPCLIASGLFEISRKLYGEIGPAFYGLRTTLLTLLLMALLRIKRPEQLKEKDPA
                     AFGRLLGLDRAPEVKTLRRRLTRLAARHCAEQLGAELARRRVDQRGHLMGFLYIDGHV
                     RAYHGQRVVSSNAYVARRHLAMPACTDYWINDSSSDPLLVITGEVDAALSKAMPRLLR
                     EVREVVGERRVTIVFDRGGWSPKLFAAMIKDGFDVLTYRKGRCRRINERRFIRRRAKF
                     DGRLVDYLLHDQPVRFLKGRLRLRQVTRLCDDGHQTTVITSRWDLRDIEVAYRMFERW
                     RQENFFKYMREEFLIDALVDYQIEPEDPTRIIPNPERRALDKEIRAARAELAGLEREL
                     GAAAVNNAEQRRPTMRGFKIAHSRLGKQLRKARARVSRLFDQLRNVPRRVEVRELNER
                     AVVKLATERKHLTDIIKMVAYQAESDLLALLRPHYARVDQEGRTLLHELFATAGDIRV
                     SDSELNITLAPLSSPHRTHAAQALCAILDQTATMFPGSRLRIRFAMRPPPRIGLAFPG
                     SPAERSTAAHSPPAP"
BASE COUNT          398 a          717 c          721 g          408 t
ORIGIN      
        1 gtcctcggct ctgcgcgggg tccgcgccgc attcatgaat atggcttata ggttcatgca
       61 tatttttggc ctactcgcca agcagtgcag ccgctaaatt cagacctcct gacatagggt
      121 agcagcaaaa tgtccggttt cggggtcgat cagggcgccg ggggactatg cgccgcggtg
      181 ctacgctcgg ccggagagcc ggggaaggca agcccgatcc gcggcggcgg acgcatggcg
      241 aagcggatcc gcaggcgaga accggggaac attgttgccg tctggtcgag gatcgcacag
      301 agcgcctggg ctgcgtgcgt gcgatgtggg gagctgagcg gagccaaggt gatattgagc
      361 tcactatcgg agacgcggat gtcgccggcg gtggcgaaca gctcatgtag cagggttcgc
      421 ccctcttgat cgacacgcgc gtagtgaggc cgcaggagcg caagcagatc gctttcggcc
      481 tgataggcaa ccatcttgat gatgtctgtg aggtgcttgc gttcggtggc gagcttgacc
      541 acggcgcgct cgttaagttc tcgcacctcg acgcgcctgg gaacgttgcg gagctgatcg
      601 aagaggcggc tcacacgcgc gcgggccttg cgcagctgct tgccgagcct gctatgggcg
      661 atcttgaagc cccgcatggt cggccgacgc tgttcggcat tattcacggc ggcggcaccc
      721 aattcgcgtt cgagtccggc cagttcggcg cgggccgcgc ggatctcttt gtccaaagcg
      781 cggcgctccg gattggggat gatgcgggtt gggtcctcgg gctcgatctg gtaatcgacc
      841 agcgcgtcga tcaggaactc ttcacgcatg tatttgaaga agttctcctg ccgccagcgc
      901 tcaaacatgc ggtaagcgac ctcgatgtcg cgcaggtccc atcggctggt gatcactgtc
      961 gtctgatggc cgtcgtcgca caaacgcgtc acttgtcgca accgcagcct gcccttgaga
     1021 aagcgcacgg gctgatcgtg cagaagatag tccaccaggc gcccgtcaaa cttggcgcga
     1081 cggcggatga accgccgctc attgatgcga cgacaccggc ccttgcgata ggtgagcacg
     1141 tcgaagccgt ccttgatcat cgcggcgaac aatttcgggc tccagccgcc gcggtcgaag
     1201 acgatggtga cccgtcgctc gccgaccacc tcccgtacct ctcggagcag acggggcatg
     1261 gccttcgaca acgcagcatc gacctctccg gtaatcacca gtagggggtc gctggagctg
     1321 tcgttgatcc agtaatcggt gcaggcgggc atggccagat ggcggcgtgc gacgtaggca
     1381 tttgaggaga cgacgcgttg gccgtgatag gcgcgcacat ggccgtcaat atacaaaaac
     1441 cccatgagat gtccgcgctg atcgacgcgc cgacgcgcga gttccgcccc cagctgctcg
     1501 gcacaatgcc gcgccgcgag gcgggtaagc cggcgccgca gcgtcttcac ttccggcgca
     1561 cgatcgagcc caagcagtct cccgaacgcc gccgggtctt tctccttgag ctgctcgggt
     1621 cgcttgatgc gtagcagcgc catgagcagg agcgtcagca acgtcgtgcg taggccataa
     1681 aaggccggac cgatttcgcc gtagagcttg cggctgatct caaacagccc gctcgcgatc
     1741 agacacggga gagccaacag gacaccggca ccgggaaccg aggacccctc gcaaaacagc
     1801 ggtgcggcat cctcgagcag cccatacaag gcgagctgcc gatcaagcgt ccgatcgctt
     1861 gcgtcacggt cgaggctcat cggcacccgc tcatcgtcgt tcacagcctc aggcgcggtc
     1921 accgtctcct gcgccgattc cgtggcgcga tctccatctt caccgctcgg cgcggcagac
     1981 ggcacgccac tcgccgccgg cttgcccgcc tcggtcgcga tcgcaggcaa cacaagctgt
     2041 gcgctctcgg cggccttcga aggtccgacc agcttgcgga tggccttctc ggtgacgccc
     2101 agccggtgcg cgatcgcacg attgctcatc ccctggctct tgagcatttc gatgctgcgc
     2161 agacgtttac cggagatccg ccgtcggccg ggacgccatc cttgctctcg accaagcgct
     2221 gccatcccgc cgttcgcata gcgc
//
LOCUS       DATLJT010000555         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1182278,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000555 DATLJT010000000
VERSION     DATLJT010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182278"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VM910_12800"
     CDS             complement(<1..1039)
                     /locus_tag="VM910_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI63446.1"
                     /translation="MSKNMLDGGQVIVDYLVREKVPYVFGLCGHGNIGFLDALYERSD
                     DIKTVSVHHESVAGFMADVYYRVGGKPTATFTSCGPGSANLPIALANSYLDSVPFLAV
                     TGNVPTSQFNRGAFQELYRHYQADFPSTVRAYCKRVFQPTRGDMVPLAVRQAWKTMVT
                     GRPGPVVLDVPFDIFKEAAAEETPKPEEWNANISCRCGADPEGVAKAVDMLLAAKRPV
                     ILVGQGVKYGGAAADLLRLAERLRIPVAWSASGTGAIDSRHPLALGLVARNGPYQANH
                     AARTADVLLALGVRFDDRTSSSWLPGYSFTIPPTRLIHVDIDPDEIARNYPVALGLMA
                     DVRTFLHQVLAE"
     gene            complement(1115..2104)
                     /locus_tag="VM910_12805"
     CDS             complement(1115..2104)
                     /locus_tag="VM910_12805"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63447.1"
                     /translation="MRNRFRWGLVAWAVFGAAALAQAQTPSSYPDRPIRLVIAFPPGG
                     ATDTLSRQVTQELGEALGQSVVIENKPGAGGYIAWNYVAAADPDGYTLLMAENALGIS
                     QALYKKTSSSFDPVKQYDAIAVVATSPLVLAVANNVKANSVNELIAYARTMPQKINYA
                     SAGIGSVSHLTFEVIKAGTGMDAVHVPYKGGGPAMNDVIAGHVALNMASIQVAKGLIE
                     AGKIKGLAVTSAERSPVLPNVPTLKEAGVKTADVDLRFWFGLFAPKGIPDAIKTKLDK
                     AVATTLSNPQVRERLANLDIEPAYAPGDILKTKLENEIANWTKFIDAHGIKPE"
     gene            complement(2310..3134)
                     /locus_tag="VM910_12810"
     CDS             complement(2310..3134)
                     /locus_tag="VM910_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63448.1"
                     /translation="MVEERTPAIPSVRGIDLSGLWSILVMSQGVLVLPVATAFACLVA
                     WQAIAVYGEISPTILPPPTMVLQQLVLNFWLILKHTIPTTYETLLAFGISIPLGIMLA
                     GLMVYSRLAYQALYPNIVFFQLIPKIALAPLFIVWLGIGAPSRVTFSIFISFFPILVA
                     TATGLQSVETTMLRLCRSLRMSQWQILIRVRFPTSFPFIFSGMKVAVTLAIIGIVVGE
                     FIASQQGLGYLILFASSRQQTDLALACIAVLCVVGIALYGCVSAFEKLLMRWYDTR"
     gene            complement(3137..>3761)
                     /locus_tag="VM910_12815"
     CDS             complement(3137..>3761)
                     /locus_tag="VM910_12815"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63449.1"
                     /translation="AGLEAVTEGTILASGTHMTGPRAEIGIVFQDPTLLPWKTALQNV
                     LFPIEMMGRSRSAYLARAMELLRLVHLDDAIHKRPRELSGGMRQRVSLCRALIADPSV
                     LLMDEPFSALDAITRDDMNIVLLELWERYRKTVLFVTHSIREAVLLADRVLVMGRSPS
                     QIIADIKVPFARPRDFSLGETEQFNELCAVLRREIEIARWGEAVTRH"
BASE COUNT          749 a         1106 c         1211 g          695 t
ORIGIN      
        1 gctccgccag cacttggtgg aggaaggtgc gcacgtccgc catcagcccg agcgcgaccg
       61 gatagttgcg cgcgatctcg tccgggtcga tgtcgacgtg aatgagtctc gtcggcggga
      121 tggtgaacga atagcccggc agccacgagc tcgaggtgcg gtcgtcgaag cgcacgccga
      181 gcgcaagcaa gacgtcggcg gtgcgcgcgg cgtggttggc ctgataggga ccattgcggg
      241 cgacgaggcc gagcgccaag gggtggcggg aatcgatggc gccggtgccg ctcgccgacc
      301 atgcaaccgg aatgcgcaag cgttccgcta gccgcaacaa gtccgccgcg gcgccgccgt
      361 atttgacgcc ttggccgacc aggatgaccg gccgcttggc cgcgagcagc atatcgacgg
      421 ctttcgccac gccctcggga tcggcgccgc agcggcagga gatgttggcg ttccattctt
      481 ccggcttcgg agtttcctcg gcagcggctt ccttgaagat gtcgaaggga acgtcgagca
      541 cgaccggtcc cggccgtccc gtcaccatgg tcttccacgc ttgacgaact gcgagcggaa
      601 ccatgtcgcc gcgcgtgggc tgaaacacgc gtttgcaata ggcgcgcacg gtcgacggaa
      661 agtccgcctg atagtggcga tagagttcct gaaaggcgcc gcggttgaat tggctggtcg
      721 gcacgttgcc ggtcaccgcc aagaagggaa cggagtcgag ataggaattg gcgagcgcga
      781 tcggcagatt ggccgaaccc ggaccgcagg aggtgaacgt cgccgtcggc ttgccgccca
      841 cgcggtagta cacgtccgcc atgaagccgg cgacgctctc gtggtggacg gagacggttt
      901 tgatgtcgtc ggagcgttcg tagagtgcgt cgaggaagcc gatattgccg tggccgcaca
      961 ggccgaacac gtagggcacc ttttcgcgca cgagatagtc gacgatcacc tgaccgccgt
     1021 cgagcatgtt cttggacatc ggcattggtc ttcgctgtta ttccggtttg atattcgcgt
     1081 ccctgatgat cttcctatag cgcgccagcg cggctcattc cggcttgatg ccgtgggcgt
     1141 cgatgaactt cgtccagttt gcgatctcat tctcgagctt cgtcttgaga atatcgcccg
     1201 gggcgtaggc cggctcgata tcgaggttgg ccagccgctc gcgcacctgc ggattcgaca
     1261 gcgtggtcgc aaccgccttg tcgagcttgg tcttgatggc atcaggaatg cctttggggg
     1321 cgaagaggcc gaaccagaag cgcagatcca cgtccgcggt cttgacgccg gcttctttca
     1381 acgtcggaac attgggcagc acgggtgaac gttcggcgct ggtgaccgcg agccccttga
     1441 tcttgcccgc ttcaatgagg cccttggcga cctggatcga tgccatgttg agtgccacgt
     1501 ggcccgcgat cacgtcgttc atcgcgggcc cgccgccctt gtagggaacg tgaaccgcgt
     1561 ccatgcccgt gcccgccttg atcacttcaa aggtcagatg cgagacgctg ccgatgccag
     1621 cggaggcgta atttattttc tgcggcatcg tgcgtgcata cgcgatgagt tcattcaccg
     1681 aattggcttt gacgttgttg gcgacggcca gaaccagcgg cgacgtcgcg accaccgcga
     1741 tcgcgtcgta ctgcttgacc ggatcgaagg acgacgaggt cttcttgtag agggcttggc
     1801 tgatcccgag cgcgttttcc gccatcagca gcgtgtaacc gtccgggtcc gccgccgcga
     1861 catagttcca agcgatgtat ccgcccgcgc ccggcttgtt ctcgatgacg acggactggc
     1921 cgagcgcctc tcccagctct tgggtgactt ggcgggacaa ggtgtccgtt gcgccgccgg
     1981 gcgggaatgc gatcaccagc cggatcggtc ggtccggata ggacgagggt gtctgcgctt
     2041 gcgccaacgc cgcggcgccg aacacggccc atgcaaccaa gccccatcga aagcgattgc
     2101 gcatggtccc cgcctcccgc gttgcgtcgg ccggttcatt cccggtactc ggaaaaccca
     2161 ttggatcacg cgcggccgtc tgcgtcgact ggttcgaacc tgggaacagg gacgtcaccg
     2221 cgacgcgttt gggatcgacc tccccgcggg agacgacgta gtcgtaagcg gcggtgccgc
     2281 agcctttcga gggttacacc gaagcggggc tatcgggtgt cgtaccagcg catcagaagc
     2341 ttttcaaagg cagagacaca gccatagagc gcgatcccga cgacacacag aactgcgatg
     2401 caggcgagcg cgagatcggt ctgctggcgc gatgaggcaa aaagaatgag atagccgagg
     2461 ccctgttgcg aggcgatgaa ctcgccgacg acgatgccaa tgatcgcgag cgtcaccgca
     2521 accttcatgc cgctaaagat gaaggggaac gacgtcggaa aacgcactct tatgagaatt
     2581 tgccactgcg acatgcgcaa ggagcggcaa agccgcagca tggttgtctc cactgattgc
     2641 agtccggtgg cggtggcaac caagatcggg aagaacgaaa taaagattga gaatgtgact
     2701 cgcgagggag cgccgatgcc cagccacacg ataaacaatg gcgccaatgc aatttttggg
     2761 atcaattgga agaacacgat gttggggtaa agagcctggt aggcaagcct agagtacacc
     2821 atcaggccgg caagcatgat gccgagcggg atcgagatgc caaacgcgag caacgtctca
     2881 tacgtggttg ggatggtatg cttcaaaatc agccagaaat tgaggaccaa ctgctgcagc
     2941 accatcgtcg gcggcggtag gatggtgggc gagatctctc cgtagacggc aatggcctgc
     3001 catgcgacca gacatgcaaa ggcggtcgct actggcagga cgagcacccc ttgggacata
     3061 accaatatcg accacaggcc gctcaagtcg ataccgcgga cggaaggtat ggctggtgtg
     3121 cgttcctcga ccatgctcaa tgcctcgtca cggcttcgcc ccagcgcgcg atctctatct
     3181 ctctgcgtaa taccgcgcac aattcattga actgttcagt ttcgccgagg gaaaaatcgc
     3241 gcgggcgggc aaacggtacc ttgatgtcgg cgatgatctg cgagggactg cgtcccatga
     3301 cgagcacgcg atcagcgagc agcacggcct cgcggatact atgcgtcacg aagagcactg
     3361 ttttgcgata gcgctcccag agttcgagca gcacgatgtt catgtcgtct cgggttatgg
     3421 catcgagcgc gctgaacggc tcgtccatca gcagaacgct cggatcggca atgagcgcgc
     3481 ggcagagcga tacccgctgg cgcatcccgc cggaaagctc ccgagggcgc ttgtggattg
     3541 catcgtcgag gtgcaccagc cgcaaaagct ccatcgcacg agcgagataa gccgagcgac
     3601 tgcggcccat catctcgatc ggaaaaagta cattttgtag agccgtcttc cagggcagca
     3661 aggtcggatc ctggaatacg ataccgattt ccgcgcgcgg cccggtcatg tgcgtgccgc
     3721 tggcaagaat cgttccctcg gtgaccgctt caaggccggc a
//
LOCUS       DATLJT010000556         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3479720,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000556 DATLJT010000000
VERSION     DATLJT010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479720"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VM910_12820"
     CDS             <1..177
                     /locus_tag="VM910_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI63450.1"
                     /translation="QWDEFRLHVRAALAEGGFTADDIKEILLQQAIYCGVPAANHAVK
                     EASTVLEELGLLKR"
     gene            complement(139..672)
                     /locus_tag="VM910_12825"
     CDS             complement(139..672)
                     /locus_tag="VM910_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63451.1"
                     /translation="MTTNNWRISSFNGVLLAAYFIPTWTIIAFRIMVSPIHSFYERPN
                     VSLAFFVSDYLQLASVGTVRAAWLLALGRLTVVAFFAIFLFLVSRAQIRKTGGSDEAL
                     AIALGIGSLISFAMMLMASQVGEIQAMRLHATELLLLLGNVIVLAVEEPLVKAFSDRN
                     ATAQSLTLEQPQLLKHG"
     gene            complement(862..1008)
                     /locus_tag="VM910_12830"
     CDS             complement(862..1008)
                     /locus_tag="VM910_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63452.1"
                     /translation="MSNPNQNPGQQTQNPNQKPGQQGGGQPKPGQQQQGGQPKPGQQQ
                     QPNR"
     gene            1375..2292
                     /locus_tag="VM910_12835"
     CDS             1375..2292
                     /locus_tag="VM910_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663086.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HVI63453.1"
                     /translation="MDYFAQQLINGLVLGSIYGLIAIGYTMVYGIVGMINFAHGDIFM
                     IGGFIALISFLVLVSFGLTAVPVILLIVLLVSMAITALYGWTVERIAYRPLRHSFRLA
                     PMLSAIGMSFVLSNYSQVAQGARVKPVPPIITGGHTLHTGAGGFVVQLSNIQIVVVVT
                     TVVLLAIFTWLVARTRLGRDMRACEQDQTMAALLGVDVDRTISMTFVIGAALAAVAGM
                     MYLLYYGLVDFFMGFVAGIKAFTAAVLGGIGSLPGAMLGGLAIGLIETLWSAYFSVEY
                     KDVAAFSILIVVLIFLPTGLLGRPEVEKV"
     gene            2310..>2936
                     /locus_tag="VM910_12840"
     CDS             2310..>2936
                     /locus_tag="VM910_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3382 domain-containing protein"
                     /protein_id="HVI63454.1"
                     /translation="MSAPEQAQASRAPGIPFILKKALISAFVALVLFSLMVGVRTEAG
                     PTGQLIYWTRFGELAALVAAVFGGSIVIELLRQWIGPAGATTLVPKQVQSALAIAGRF
                     VGPALLIFTLLVPVIFYNQRYILDLGILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYA
                     VGAYSYALIATTFGLSFFECLPLAGFLAALWGVMLGFPVLRLRGDYLAI"
BASE COUNT          473 a          903 c          927 g          633 t
ORIGIN      
        1 caatgggacg aattccgcct gcatgtgcgc gcggccctcg ccgagggcgg ctttacggcc
       61 gacgacatca aggaaattct cttgcagcag gcgatctatt gcggcgtgcc ggcggcaaat
      121 cacgccgtca aggaagcctc aaccgtgctt gaggagctgg ggctgctcaa gcgttagact
      181 ctgcgccgtc gcattgcgat cggagaacgc ctttaccagc ggctcctcca cggcgagcac
      241 gatcacattg ccgagcagca acaacagttc ggtcgcatgc agtcgcatcg cctggatctc
      301 gccgacctgc gaggccatca gcatcatggc gaagctgatc aggctgccga taccaagcgc
      361 gatggccagc gcctcgtcgc tgccgcccgt tttgcggatt tgcgcccggc tcacgagaaa
      421 gaggaatatc gcgaagaagg cgacgacggt cagtcgaccc agcgccaaca gccaggcggc
      481 gcggacggtg ccgacggacg caagctgcaa atagtcgctg acgaaaaatg caagcgagac
      541 gttgggccgc tcatagaaac tgtgaatcgg cgaaaccatg atcctgaacg cgatgatggt
      601 ccaggtcggg atgaaatagg cggcgagcag cacgccgttg aatgaactga tccgccagtt
      661 gtttgtcgtc atgtcgcttc cctcgccgcg gcgttttgga tcgggtgcgc ggcctttgcg
      721 gcgaggtaac ctgcttaaag tgcgaacggc aatttaaacc gtttgtttac cttaattgcc
      781 cgggcgtccg gccgacgcgg attgtggcaa aagaaaagcc ccgccattcg gcgaggctct
      841 tccggtccga cttctcccga actagcgatt gggttgctgc tgctgaccgg gcttcggctg
      901 cccgccctgc tgctgttgtc cgggcttcgg ctgtccgcca ccctgctggc caggcttctg
      961 gttggggttc tgcgtctgct ggcccggatt ttgattggga ttgctcattc gggggtctcc
     1021 tttgttgaat gcagccgggg ttaacttggg cgggaggaat tggttgcggc gggaactttg
     1081 ggttcgacgg gaacatcgca cagcaccggc cggtcaggct tggggagccg gaaccggcag
     1141 caaaaacaat cctgtagatg ccgatcctgc ggcggcacgc gctgttgccg cccgcggttc
     1201 cgaactgtta gaaaccggcc acgacgcgtc gctgaaaggc ccgccgcgtc attgcagcag
     1261 cgttttcaag cgaagtgggt gccggttcgc gtgaagaaaa cgcatcaaac aaagattggg
     1321 accgcgccgg cccctgatct gatcagaacc ggcgaggcgg cgtagcggca gcccatggat
     1381 tatttcgcgc agcagctcat caacggtctc gtgctcggct cgatctacgg cctgatcgcc
     1441 atcggctaca ccatggtcta tggcatcgtc gggatgatca atttcgcgca cggcgacatc
     1501 ttcatgatcg gcgggttcat cgcgctgatc tcgtttctcg tcctggtctc gttcggtttg
     1561 accgcggtgc cggtgatcct gctgattgtg ctgctggtct cgatggccat caccgcgctc
     1621 tatggctgga ctgtggagcg gatcgcctac cggccgctgc gtcattcttt ccgcctcgcg
     1681 ccgatgctgt cggcaatcgg catgtcgttc gtgctgtcga actattcgca agtcgcgcag
     1741 ggcgcgcgcg tcaagccggt gccgccgatc atcaccggcg gccatacgct gcacacgggc
     1801 gccggcggct tcgtcgtcca gctctccaac atccagatcg tcgtcgtcgt cacaaccgtg
     1861 gtgctgcttg cgatcttcac ctggctcgtg gcgcgcacgc ggctcgggcg cgacatgcgc
     1921 gcctgcgagc aggatcagac catggcggcg ctgctcggcg tcgacgtcga ccgcaccatc
     1981 tccatgacgt tcgtgatcgg cgccgcgctc gcggcggtcg cgggcatgat gtacctgctc
     2041 tattacggtc tggtcgattt cttcatgggc tttgtcgccg gcatcaaggc gttcaccgct
     2101 gcggtccttg gaggcatcgg ctcgctgccc ggcgcgatgc tcggcgggct tgcgatcgga
     2161 ctgatcgaga cgctgtggtc ggcctatttt tcggtcgaat acaaggacgt cgcggcgttc
     2221 tcgatcctga tcgtggtgtt gatcttcctg ccgacgggcc tgctcggccg acccgaggtg
     2281 gaaaaggttt gaaaagcggc agcgccacga tatctgcccc cgaacaggcg caggcttcgc
     2341 gtgcgccggg cattcccttt attctgaaga aagccctgat cagcgccttc gtcgcgctcg
     2401 tgctgttctc gctgatggtc ggcgtccgaa ccgaggcggg accgaccgga cagttgatct
     2461 actggacgcg ctttggcgaa ttggcggcgc tggtcgcagc cgtgttcggc ggcagcatcg
     2521 tgatcgagtt gctcaggcag tggatcggtc cagccggcgc aaccaccctc gttccgaaac
     2581 aggtgcaaag cgcgcttgcg atcgcgggcc gtttcgtcgg cccggcgctt ttgatcttca
     2641 ccctgctcgt cccggtgatc ttctacaacc agcgctatat tctcgacctc ggcatcctcg
     2701 tgctcaccta cgtgatgctg ggctggggat tgaacgtggt ggttggcctc gccggcctgc
     2761 tcgatctcgg ctacgtcgcc ttctacgcgg tcggtgccta ttcctacgcg ctgattgcga
     2821 ccactttcgg cctctcgttc ttcgagtgtc tgccgctcgc cggatttctt gccgcactgt
     2881 ggggcgtgat gctgggcttt ccggtcctgc gtctgcgcgg cgactatctc gccatc
//
LOCUS       DATLJT010000557         6287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1641669,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000557 DATLJT010000000
VERSION     DATLJT010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641669"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VM910_12845"
     CDS             <1..199
                     /locus_tag="VM910_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63455.1"
                     /translation="RLLCLGLSFSADTLFTRRCFVVLVQRRLPPIRKASLVKVGWRDL
                     APLDAAVDGSRMYVTVTLRHL"
     gene            complement(1036..2256)
                     /locus_tag="VM910_12850"
     CDS             complement(1036..2256)
                     /locus_tag="VM910_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI63456.1"
                     /translation="MAKLLTALAVKNLKPRDKRYSVPTGAANGLFVNVLRSGHKSYSI
                     RYRFGGRTRNLTLNDGLTLAEARAQAGQALLEVSRGVDPAAVKVDAKAKAKLATTNTG
                     DDSIEKHAEQFLELHARRKTGKANAKQAELALYRHALPAWRGRDVQSIRRRDIIEVIE
                     AVAHDQGHPVMANRVLGHLSKFFSWLVARDVIAGSPCIGVERPKKEKARSRVLNDNEI
                     RDFWTATTKLSAPFGDIYRLLLLSAARRQEVAEMRWSEVDLVNKVWRLPRERTKNDEG
                     AALPLGPTAWAVIEAQPRIVGVDFVWGARRTGFSHFKSELDAAMQPAVPWRNHDLRRT
                     SRTLLARAGVIDAVAERCIGHKVGSAVSQIYNQHRYVEEMRHAFAALESLIGTIISPT
                     PAKVVTLPRRKLRS"
     gene            2438..2731
                     /locus_tag="VM910_12855"
     CDS             2438..2731
                     /locus_tag="VM910_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63457.1"
                     /translation="MDIAVGEIAEDLGAAALERNVHEIKTGAFGEDFGIDLLIAADAG
                     ATVTDLARVFAGVGEKVGERRGGKTRRGREEVVVARKKTGTSASGATISTSRS"
     gene            complement(3181..3336)
                     /locus_tag="VM910_12860"
     CDS             complement(3181..3336)
                     /locus_tag="VM910_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63458.1"
                     /translation="MPIELIMAAPVIAYAVTVALAFGRLLVSPRRLRAAHVGGRDRRM
                     IGHPTTT"
     gene            complement(3321..3650)
                     /locus_tag="VM910_12865"
     CDS             complement(3321..3650)
                     /locus_tag="VM910_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI63459.1"
                     /translation="MQMHQIRYFLALCEERNFTRAAGRCGVSQPSLTNAIIALERELG
                     GALFQRKPLIALTTLGRAIQPYLDRIAQNAAHAREAAQALADVPPANSEPASREDLAG
                     SPLCPSS"
     gene            4016..4750
                     /locus_tag="VM910_12870"
     CDS             4016..4750
                     /locus_tag="VM910_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI63460.1"
                     /translation="MSIPGGKRPAERTRDKLSLLRNHSLFRDLPSAVLEHLGSYMKTR
                     RVARGNTIFAKGDPGTGLMGVLAGTVKVSVASAEGKDIVLNLFHEGEVFGEIALLDGR
                     PRTADAVAMSDCELVVIERRDFVPFLSSHPDVMLKFIEILCSRLRRTSEQVQDITFLN
                     LPTRLAKTLLQLTAAEDGSATTRKAAITQREISQMIGISRESTNKQLRAWAKRGWIRL
                     ERGGVNVVAPDKLAAIATEGSEFDRS"
     gene            complement(4791..5405)
                     /locus_tag="VM910_12875"
     CDS             complement(4791..5405)
                     /locus_tag="VM910_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI63461.1"
                     /translation="MRNDFAELVPGIAAERRSPTAKDNSDEALAAKIASGSRLAMQVL
                     FARHHARIYRFVLRLLGSEALAEDVTSEVFLGVWRQAHRFEARSAVTTWLLAIARYKA
                     LAELRRRPEACDEASVDASDPADDPEATFAVKHRGEILRNCLTRLSPRHREIIDLVYY
                     HEKSVQEAAHILGVPGNTVKTRMFHARKKLSELLEAQGVVGALS"
     gene            5731..>6287
                     /locus_tag="VM910_12880"
     CDS             5731..>6287
                     /locus_tag="VM910_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63462.1"
                     /translation="MPRWTTGLLAALLLAGVGSAHAQTYPSATVKLIVPVPAGGVTDT
                     MARIVAQRLTEAWGQPVVVDNRPGGNYAVGAQAVAKSPPDGLTLLVAPDSTVTANPHL
                     FSKLPYDPVKDLTPIIVLCRITPVLVINPSLPVQSVQELIALAKAKPGSLNYGSYGIG
                     TYAHLSMEDFKQRTGTDIVHIPYRGA"
BASE COUNT         1116 a         1950 c         1973 g         1248 t
ORIGIN      
        1 ccgccttctc tgtctcggcc ttagcttctc ggcggatacg ctcttcacgc gccgctgctt
       61 cgtcgtgttg gtccaacgtc gactcccacc aataagaaag gcgtccctgg tgaaagtcgg
      121 ctggcgggat ctcgccccgc tggacgcggc ggtcgacggt tcgcgtatgt acgttaccgt
      181 aacgctccgc cacctgtgat ttgccgaaag ctctgtttgg cttcggatgc tgtccatatg
      241 gatgttcacg attgctcgcc agtgctcgac gagcggcacg gttcgggtga gcgacttcca
      301 tggaaaaaaa cctccacgtc gtttgatgtc gcgacgcgaa gtttagcgga gccatatgga
      361 agtctatccg gaagtgtccg caaatgcgga aaaggcggaa aatgcggatt atcttttagg
      421 caacttgcgc attgcggctt taactgtatc ccaaccggga tggaaatccc cctccttgtc
      481 cctgtcggaa accctcttgc gcacgtcggt cacagatgtg ccgggtggcg gtacaccgtc
      541 aggtcgatat gccaagcgca ttctgcggat gatttctttc tgcactcgcg gcagttcctt
      601 cgccccgctg ccggcacggt tgggctgtga tgctgtaccc tgcttggccc ccttggatgg
      661 tccgggccgc gccatataaa tcgcgcaccg gtggtgttcc tcagacgaga ataggcctcc
      721 tccaaggggc catccccatc caccattggc gaattctacg gctctctccc ctcgccaata
      781 cgctcgcgga atctcatgat attcgccggt tgcatagtgc tgccaccaag caaccagatc
      841 gccgttgatc agatcggctc tctgacgatc caaccctaac tcatcgtgcc aatccgcagg
      901 cggccttagg tccttgaccg gaacggagcc agcgctgctc tctggtatat tcttcccagc
      961 cttagccatt ggaccaccga tggcttgggg taaggccagc ctggacggtt tgagcccgtc
     1021 caggctggcc gcttttcagc tccgcaattt ccgtcgcggc agtgtgacga ccttggccgg
     1081 tgtcggactg atgatcgtgc cgatcagact ttccagcgcc gcgaaggcgt ggcgcatttc
     1141 ctcgacatat cggtgctgat tgtagatttg gctgacggcg ctgccgactt tgtggccgat
     1201 gcatctctcg gcaaccgcgt cgatgacacc ggcgcgggcc agcagggttc tgcttgttcg
     1261 ccttaaatca tgatttcgcc aaggcacggc gggctgcatc gccgcgtcaa gttcggattt
     1321 gaagtgtgag aaaccggtgc ggcgagcgcc ccacacgaaa tcgaccccca cgatgcgcgg
     1381 ctgcgcctcg atgaccgccc acgccgtcgg ccccagcggc agcgcggcgc cttcgtcgtt
     1441 cttcgtacgc tcgcgcggca atctccaaac cttgttgacg agatcgacct cggaccagcg
     1501 catttcggca acttcctgcc gacgagccgc gctcaacaac aataaccgat agatgtcacc
     1561 aaacggtgct gacaacttcg tggtcgcggt ccaaaaatcc ctgatctcgt tatcattcaa
     1621 gacgcggctg cgggctttct ctttcttcgg cctctcgacg ccgatgcatg gcgagccagc
     1681 gatgacgtcg cgggcgacaa gccacgagaa gaacttggac aaatggccga gcacgcgatt
     1741 ggccatgacg ggatggccct ggtcgtgggc gacggcctcg atgacctcga tgatgtcgcg
     1801 gcgtctgatg ctttggacat cgcgtccgcg ccaagcaggc agcgcatggc gatagagcgc
     1861 aagctcggct tgctttgcgt tggccttgcc cgtcttgcgc ctagcgtgca gttcgaggaa
     1921 ttgctcggca tgtttctcga tgctgtcgtc gccggtattg gtggtggcga gcttcgcttt
     1981 cgccttcgcg tccactttga ccgctgcagg atcgacgccg cgagacactt cgagcaaagc
     2041 ttgaccggct tgcgctcgcg cttccgccag cgtgaggcca tcgttgagcg tcaggttgcg
     2101 cgtccggccg ccgaagcgat agcggatgct gtaggacttg tgaccggacc gcagcacgtt
     2161 cacgaagaga ccgttagcgg cgccggtcgg gacggaatat cgcttgtcgc gcggcttgag
     2221 gttcttcacc gcgagggcgg tcaacagctt tgccatgaaa tgttactccg agggcttgag
     2281 gagtaacgtt tcgaggtgtc tagcggcgtt actccgtgtc cctcagtgtc gtctaatgac
     2341 gtcaagaaag caaggaagca tggggacaac ttgtcgcccg gcgtcgtggt atgtcatccc
     2401 ctgtctaccc cgccacggtg atgttgaacc acgacacgtg gatatcgccg ttggcgagat
     2461 cgcggaggat ctcggcgccg ccgcccttga acggaatgtg cacgagatca agaccggcgc
     2521 gtttggcgag gatttcggta ttgatctgct gatagctgcc gacgccggcg ctaccgtaac
     2581 ggaccttgcc cgggtgttcg cgggcgtagg cgagaaagtc ggcgaacgtc gtgggggaaa
     2641 aactcgtcgt ggtcgcgagg aagtcgtggt cgcgaggaag aagaccggga catccgccag
     2701 cggggcgacg atctcaacgt cccgctcgta atcgattttc atctttttcg cgagcaggac
     2761 cggcgtcagc gcattggtgg agatgttgcc gatcatcagg gtgtggccgt ccggccgggc
     2821 gcgggccatc tcctcgatcg cgatgatgcc cgacgcgccg ggcttgtttt ccacgaacac
     2881 gccccggccg aagctctgtc gcaactgctc ggaataaaga cgcgcaacca cgtcggtcaa
     2941 accgccgggc gcgtagggca cgatgattct gactggcttc gacggatagg tttgcgccgg
     3001 cgcctgcgct gcgatgacga cgagaccaaa tgcgcacacc acggcccgac ccaagcgccg
     3061 catgtggtgt tcctccgccg gcgctctcga agcgcggctc tcacttgccg gcggattgta
     3121 tgaacgcctt atgccattgc acaagggtga agcgatcgcg tgatgcgcga gcgcggcgct
     3181 ttacgtcgtt gtcggatgac cgatcatcct tcgatcgcgg ccgccgacat gagccgcgcg
     3241 cagacgccgt ggcgacacga gcagacggcc gaaagcgagc gccacggtca cggcataggc
     3301 gatgactggc gcggccatga tcaactcgat gggcataacg ggctccctgc gaggtcctcg
     3361 cgcgacgcgg gctccgaatt cgccggcggc acatcggcca gcgcctgcgc ggcctcgcgc
     3421 gcatgcgccg cgttttgagc gatccggtcg agataaggtt gtatggcgcg gccgagcgtg
     3481 gtgagggcga tgagcggctt gcgttgaaaa agcgcgccgc ccagttcgcg ctccaacgct
     3541 atgatggcgt tggtcagcga gggctgggag acgccgcagc gcccggccgc tcgcgtgaaa
     3601 ttgcgctcct cgcagagcgc caggaagtag cggatctggt gcatttgcat gggtccacct
     3661 cgcgggatca atcgcgctcg cgtgggagcc tgccatcgac atgggcgttt gacggtgagg
     3721 tggttcacag agcggcgctt ttcctcgctg aggcttttgt ttgcggtcga catgcgcagg
     3781 taatcggctg cggtgcccgc gaccggcagg gccttcgggc cggatgcact cccagcgtgc
     3841 atttaagcgc ttcgcttctt taaagagtgt gtccctcgca tcggcaaacg cgccaaatcc
     3901 atagccatgg gctatagtgc ccccgcttga atcgagtctc cgacaaccgg ttgtcggcgg
     3961 cgtgttcgag agcgcagttc gaattgcaag attcgtttgt gacgggggag ctctcatgtc
     4021 gattccaggc ggcaagcggc cggcggaacg cacgcgcgac aagctgtctt tgctgcgcaa
     4081 tcattcgctg tttcgcgacc ttccctcggc ggtgctcgag catctcggct cgtacatgaa
     4141 gacgcgtcgg gtggcgcgcg ggaacaccat cttcgcaaaa ggggatcccg gcaccgggct
     4201 gatgggggtg ctcgccggga cggtcaaggt cagcgtcgcg tccgccgagg gcaaagacat
     4261 cgtgctcaac ctctttcacg agggcgaggt ttttggagag atcgccttgc tcgacggtcg
     4321 accgcgcacc gccgatgccg tcgccatgtc cgactgcgag ctcgtcgtca tcgagcggcg
     4381 cgacttcgtt ccgtttctga gcagtcaccc cgacgtgatg ctcaagttca tcgaaatcct
     4441 ctgctcgcgg ctgcgccgga ccagcgagca ggtgcaggac atcacattcc tgaatttgcc
     4501 gacccgcctg gcgaagacct tgctccagct gaccgcagcc gaagacggtt cggcaaccac
     4561 acgcaaagcg gcgatcacgc aacgcgagat cagtcaaatg atcggcatat cccgcgagag
     4621 caccaacaaa caactgcggg catgggccaa gcgcggttgg attcgcctgg aacgaggggg
     4681 cgtcaacgtg gttgcgccgg acaagctcgc tgccatcgcc accgagggtt cggaattcga
     4741 tcgatcgtga gtttggatgt gtagggtggg caaaatcgcc tggtgtgtcg ctatgacagc
     4801 gctcccacga cgccttgcgc ttcgagcagc tccgagagct tcttgcgtgc gtggaacatc
     4861 cgcgttttga cggtgttgcc cggaacgccg aggatgtgcg ccgcctcctg caccgatttt
     4921 tcatggtagt agacgaggtc gatgatctcg cgatgccgcg gggacaggcg ggtgaggcaa
     4981 ttgcgcagaa tctctccccg atgcttgacc gcgaaggtcg cttccggatc gtccgccggg
     5041 tcgctggcgt ccacggacgc ttcatcgcac gcctcgggcc ggcgccgcag ctcggccagc
     5101 gccttgtaac gggcaatggc caacagccag gtcgtgaccg ccgagcgtgc ctcgaaccgg
     5161 tgggcttgcc gccagacccc gaggaacact tcgctggtga cgtcctccgc gagcgcctcg
     5221 ctgccgagca gccgcaatac gaaacggtag atgcgcgcat gatgccgggc gaacaggacc
     5281 tgcatcgcaa gcctgctacc ggatgcgatc tttgcggcca gcgcctcgtc cgagttgtcc
     5341 ttggccgtgg gcgaccgccg ctcggcggca atacccggga cgagctcggc aaagtcgttg
     5401 cgcattggta gctcccctgt tttggtctac caatatgcct agactgcccc gcggaaacct
     5461 tttgttaact acttcacaca gccgacccga gctttcaggc ccgcgtttga ccgcgacggc
     5521 ttgccgggct cgccaggttg gcggggcctg ggtgctggtc tattttccgc ttgcgcggcg
     5581 aagcatccaa aaggtcaccc ccgggcttga cccgggggtc catccttctc cgcaaaaggc
     5641 tttttcacaa cgaggatgga ttgccgggtc aggcccggca atgacgacct aaagagatcg
     5701 gccgccgcgc aaatcgatag gggagggacg atgccgaggt ggacgaccgg gctgctcgcg
     5761 gcgttgctgc tcgcgggagt gggcagcgcg catgcgcaaa cctatccgag cgccaccgtc
     5821 aagctcatcg tgccggttcc ggccggcggc gtgaccgata ccatggcccg tattgtggcc
     5881 cagcgactga ccgaggcgtg ggggcaaccg gtcgtcgtcg acaaccgtcc aggcggcaac
     5941 tatgccgtcg gcgcgcaagc ggttgcgaaa tcgccgccgg acgggctcac gctcctggtc
     6001 gcgccggact ccaccgtcac cgccaatccg catctcttca gcaagcttcc ttacgatccg
     6061 gtcaaggacc tcacgcccat catagtgctg tgccgcatca ccccggtgct ggtgatcaac
     6121 ccgtcactgc cggtgcagag cgtgcaagag ctgatcgcgc tggcgaaggc caagcccggg
     6181 agcttgaact acggctccta cggcatcggc acttatgccc atctgagcat ggaggatttc
     6241 aagcaaagga ccggcaccga catcgtgcac atcccctacc gcggcgc
//
LOCUS       DATLJT010000558         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1871023,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000558 DATLJT010000000
VERSION     DATLJT010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871023"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VM910_12885"
     CDS             <1..566
                     /locus_tag="VM910_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVI63463.1"
                     /translation="GTLAARYLTDTRHIDLAALPADVDAVLRFHPRCPFNGTYHPCLF
                     ALMRDVTTNEPTGIHRIALTPNACKIDRWSLGKLGAVKLWPAGPQLVVGEGIETVLAA
                     ATRIPYEDAPLRPAWALGSSTPLGQLPVIPGVERLILLIDHDDAGIAAADSCTQRWTR
                     ARRTVVRLMPDEAGADFNDLILAARTS"
     gene            563..1966
                     /locus_tag="VM910_12890"
     CDS             563..1966
                     /locus_tag="VM910_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase family protein"
                     /protein_id="HVI63464.1"
                     /translation="MSTSPKFSATVVTPVSPTDNEGDPEGVNLNNFFSLMNKSGAYIF
                     IPCRDIWPRASVDARLPPMPVLNKNGNPRRGSNGKIIYQPASKWLDKNRPVEMMTWAP
                     GLPLRIPDRLISLGGWIERKGVSCFNLYRPPRIKLGDASKANFWLDHVYRIYPDDADH
                     IIKWLAHRVQRPDDKINHALVLGGAQGIGKDSLLEPVKETIGRWNFLEIAPKDLFAPF
                     NPFARSVILRINEAHDLGETDRFKFYDRAKLYIVAPLDVLRVNEKYIPEHYVPNVTGV
                     VITTNHKTDGIYLPADDRRHYVAWSKCEKEQFSPDYWNALWNNYANRGFEHVAAYLIE
                     LNLSNFDAKAPPPKTPTFWEIVNVNCPPEEAEIADVIDSLGKLDANGVTIPPKTVTIP
                     ELIAAAVGETAEWLINRGNRRSLPHRLERCGYSAVPNLQAKDQLWKYKGARLAIYVQT
                     SLSPQEREAAAQEKARS"
     gene            1963..2199
                     /locus_tag="VM910_12895"
     CDS             1963..2199
                     /locus_tag="VM910_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63465.1"
                     /translation="MKKEIAPMAKNKFRRLVCGDPRAACFNLESAIRDLWCSLLVETL
                     AAERDGIRRCKNGDFPEAWYREYCRRVAPKPGTA"
     gene            2633..3058
                     /locus_tag="VM910_12900"
     CDS             2633..3058
                     /locus_tag="VM910_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63466.1"
                     /translation="MALNAKTDPNYVPKRINGKFAPGWSGNPGGSLEATRRSFNRDFL
                     LALAADFKKHGAAAIEKVREQQPAAYMKICALLVPREMKLEHAGGVKAMPDEQIEGAI
                     AAIEAWMAARMAGEGAKVIEGEAEVVPSLPAPARPTKAS"
     gene            complement(3074..>3421)
                     /locus_tag="VM910_12905"
     CDS             complement(3074..>3421)
                     /locus_tag="VM910_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI63467.1"
                     /translation="VKNGISSVHPIRGDEMRALRKLRRDYPTDAHVFVSERGGPISPI
                     GFHRLIQRLGEAAKMPFPIHPHMLRHACGFKLANDGHDTRALQHYLGHKNIQHTVRYT
                     DMAPDRFKDFWRS"
BASE COUNT          743 a         1039 c         1002 g          637 t
ORIGIN      
        1 ccggcacgct tgccgcacgc tatcttaccg acacccgcca catcgatctc gccgcgctcc
       61 ccgccgacgt tgacgcagtg ctgcgcttcc acccgcgctg cccgttcaac ggaacctatc
      121 acccctgctt gttcgcgctt atgcgcgacg tcacgaccaa cgagccgacc ggaatccacc
      181 gcatcgcgct cactcctaac gcctgcaaga tcgatcgctg gtcgctcggc aagctcggcg
      241 ccgtgaagct gtggccggca ggaccgcagc tcgtcgttgg cgagggcatt gaaaccgtgc
      301 tcgccgcggc aacgcggatt ccctacgagg atgcaccgct acgaccggct tgggcgctcg
      361 gttcaagcac tccactcggc caactccccg tgatcccggg ggtcgagcgg ctaatcctcc
      421 tcattgacca cgatgacgcc ggcattgctg ccgccgacag ctgcactcag cgctggaccc
      481 gtgcccggcg tacggtggtg cggcttatgc cagatgaagc cggcgctgac tttaacgacc
      541 tgattttagc ggcgcggacg tcatgagcac atcgcccaaa ttctccgcaa ccgtggtcac
      601 tcccgtctct ccaaccgaca acgagggcga tcccgaaggc gtgaacctca acaacttctt
      661 ctcgctcatg aacaagagcg gagcctacat cttcatcccg tgccgcgata tctggccgcg
      721 cgcgagcgtc gacgcgcggc tcccacctat gccggtgctc aacaagaacg gcaaccccag
      781 acgcggtagc aacggcaaaa tcatttacca gccggcaagc aagtggctcg acaagaaccg
      841 gccggttgag atgatgactt gggccccggg tctgccgctg cggattccgg accggctgat
      901 ctcactgggc ggttggatcg agcgcaaggg cgtcagctgc ttcaaccttt accggccgcc
      961 acgcatcaag ctcggcgatg cgagcaaggc caacttctgg ctcgatcacg tctatagaat
     1021 ttatccggac gacgccgacc acatcatcaa atggctggcg catcgggtgc agcgtcccga
     1081 cgacaagatc aaccacgcgc ttgtactcgg cggtgcgcag ggcatcggca aagactcttt
     1141 gctggagccg gtaaaagaaa caatcggtcg gtggaatttt ctggagattg cgccgaagga
     1201 tttatttgcc cccttcaatc cattcgctag gtcggtgatc ttgcgcatca atgaagcgca
     1261 tgatctcggc gagaccgacc gtttcaaatt ctacgaccgt gccaaactct acatcgtcgc
     1321 tccgctggat gtattgcgcg tcaacgaaaa atacatccct gaacactacg tgcccaacgt
     1381 gaccggagtc gtaatcacca ccaaccacaa aaccgatggt atctatctgc cagccgacga
     1441 tcgccgtcac tacgtggcat ggtcaaagtg tgagaaagaa cagttctcgc cagactattg
     1501 gaacgcgctg tggaacaact atgccaaccg tggcttcgaa cacgtcgcgg cctacctgat
     1561 cgagctcaac ctctccaact ttgacgcaaa agcgcctcca ccaaagacgc cgacattctg
     1621 ggagatcgtg aacgtcaact gcccgccaga ggaagccgaa atagcggacg tgatcgactc
     1681 gctgggcaaa ctcgatgcga acggcgtgac gatccctccc aagacggtga caataccgga
     1741 gcttatcgct gccgcggtcg gcgaaactgc cgagtggctg atcaatcgcg gcaaccgccg
     1801 ctcgcttccg caccggctcg aacgctgtgg ttattcagca gtccccaacc tgcaagcaaa
     1861 ggaccagctg tggaagtaca aaggtgcccg attagccatc tacgtgcaga cgagcctgtc
     1921 cccacaggag cgggaagccg cagcgcagga aaaggctcga tcttgaaaaa ggaaatcgcc
     1981 ccgatggcca agaacaaatt tcggcgtctt gtttgcggtg atccccgcgc cgcctgtttt
     2041 aatcttgaaa gcgcaatccg cgacctctgg tgttcactgc ttgtggaaac gctcgcagcc
     2101 gaacgtgacg ggataaggcg ttgcaaaaac ggcgattttc ccgaggcgtg gtaccgcgaa
     2161 tattgtcggc gcgtagctcc caaaccggga acggcttgac ctgaaagggt gagcggacgg
     2221 aaagggaggg cgggcaaagg ttggctggtc agtggagtca gtggagattc taagctccat
     2281 aaaccctgta cccttcaatg aacaaaaaac gcgcgcgcgc gcgtgagtta ccatacgtcc
     2341 tcgggaatag gagtaataaa aatccactga ccaccactga ccggacgctc cccgccggcc
     2401 ctcctcgagg gcgccggggg tgctgtgggc ggcggcctcg ttcataactt tgttgaaact
     2461 tttcgcgacg cccttggtct atcggtcccg gtgggaacct aaacaggaag catgagaacc
     2521 cgtccaggct cgataggcaa cctgctgggc taagctaact ctctctgcca actagcggaa
     2581 tcactcccca gtcggttatc ctcatgctct cagcaagcag aggactgccg cgatggctct
     2641 gaacgccaag accgatccga actacgtccc caaacgcatc aacggcaaat tcgctccggg
     2701 gtggtcagga aaccctggcg gttccttgga ggctacgcgt agatcgttca acagggattt
     2761 tctgctcgcg ctcgctgccg acttcaagaa gcatggggcg gccgcgattg aaaaggtgcg
     2821 tgagcaacag cctgcggcct acatgaagat ttgcgcgctc ttggtgccga gggaaatgaa
     2881 actcgagcat gccggcgggg ttaaagccat gccggacgag cagatagagg gcgcgattgc
     2941 ggcgattgaa gcatggatgg cggcgcggat ggctggggag ggtgccaagg tgatcgaggg
     3001 cgaggcggaa gtcgtcccga gcttgccggc gcctgcaagg cccacgaagg catcgtgaag
     3061 cgggcgttcg gattcaactc ctccaaaagt ccttgaatcg gtcgggtgcc atgtcggtgt
     3121 accgaacggt gtgctggatg ttcttgtggc ctagatagtg ctgaagggcc cgcgtgtcgt
     3181 ggccatcatt agcgagcttg aagccacagg cgtggcgaag catgtgcggg tggatcggga
     3241 atggcatctt ggcggcctcc ccgaggcgct ggataagccg gtggaagccg atagggctga
     3301 ttggcccgcc gcgctcggaa acgaatacgt gggcgtctgt cgggtaatcg cgacgtagct
     3361 tgcgcagcgc ccgcatctcg tcaccgcgga tcggatgtac gctggaaatc ccattcttga
     3421 c
//
LOCUS       DATLJT010000559         5557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2330809,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000559 DATLJT010000000
VERSION     DATLJT010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330809"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VM910_12910"
     CDS             complement(<1..600)
                     /locus_tag="VM910_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVI63468.1"
                     /translation="MSSNLTEEELAALMPSELCQYQTPIPTQIVSSDEFYPDSQNERQ
                     REVEARLLAMADELGGQQGLNRRKFFQTAAGMAASFVAMNEVYGPLFDVTKAEAATPA
                     MAEERAAALKDQFIMDMHTHFLRDDTRITTFLDMRKAVGKAGWNKQLSDHDQTIEDLK
                     FNNYKKEMFLDSDTKISLISSAPSDIEQDWFLTNEQMAQA"
     gene            861..1880
                     /locus_tag="VM910_12915"
     CDS             861..1880
                     /locus_tag="VM910_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI63469.1"
                     /translation="MTHLENPLVRRDVIKGAGLGLIAGTFAGALPAQAAPVEGSEVWS
                     NEYWAKKGDVPLWMYRKRLGAPKAGEPAKPVVFFVHGSSVTSRSFDLKVPGKGEYSVM
                     NEFARYGFDCWTMDHENYGKSGRTSGNSDIASGVEDLKAAAEVVTRETGQQKFHFIGE
                     SSGALRAGAYAMVAPDRINRLVFAAFTYKGEGSPTLKKRAEQLDYYRTHNMRKRDRDM
                     IRSIATRDRPGTSDVAAVEALADVELQFGDQIPTGTYLDMTANLPVVHPEKVLAPVLL
                     VRGEYDGIATVPDLEEFFNKLPNGDRQFSILPGTAHSVVLAINRQLFWHVTRAFLNMP
                     MPIAT"
     gene            2009..2488
                     /locus_tag="VM910_12920"
     CDS             2009..2488
                     /locus_tag="VM910_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI63470.1"
                     /translation="MSKICPCLWFDGEAEEAATFYVSLLPGSGIETIQKNSVDSPAGK
                     AGSVLVVEFTLAGQRFMALNGGMRMEYTHAVSFKIDCADQAEVDRLWDALTANGGQAE
                     RCGWLRDRYGVYWQIVPSALPKYLGGADRAGAQRAMQAMLGMVKLDIEGLRRAYEGA"
     gene            complement(2629..3285)
                     /locus_tag="VM910_12925"
     CDS             complement(2629..3285)
                     /locus_tag="VM910_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63471.1"
                     /translation="MSNRLNRLAPCLLVGFCAAAVMLKCEIQSASAHEVHKRTVEAHA
                     LDEAKPTHTARTPGSGSAKGSYYVDFRARTAASWGHAFVWYGRTGERQVEVAGLTPAG
                     DSLDYVLGYVMRVPSETGASYGDLDPQYLTASYRVYLNEPDAKRVFAYIKKLQASSPV
                     WNAETSNCNAFIGSIANFMGLKVPNRWERPENYVNNLKAMNGGRQMIYLSERSLASWQ
                     "
     gene            complement(3857..4261)
                     /locus_tag="VM910_12930"
     CDS             complement(3857..4261)
                     /locus_tag="VM910_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63472.1"
                     /translation="MKTRVMGFAVAALLVAAPTFRGHAEDAAAPAPAAGPTTTPAATP
                     SQPAPVSVPRPSIVPATTEPAKPAATTEPVKPVTTTEPSKPAVTDDERPRRRRHYAHR
                     HSRYAWWHPFPIYWPHLYHHRFYWSRISWFSF"
     gene            complement(4398..5048)
                     /locus_tag="VM910_12935"
     CDS             complement(4398..5048)
                     /locus_tag="VM910_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI63473.1"
                     /translation="MTSFVRFAGLKAITICAGLSMTAGLALAADNNVTKDEILGALYV
                     KPQPLTRGLSVGAPQVDPAVKAKEANFLASVRNRRTRSLSLGEREEIAEIAATKPKID
                     LEIQFDYNSAAISHSSMPSVQALGQALSDPKLRGSTFVVAGHTDAIGSDAFNQDLSER
                     RADTIKRYLVDNYHIATGELVTVGYGKSKLKNAQNPTDPINRRVQVVNMDTKTAAQ"
BASE COUNT         1047 a         1651 c         1732 g         1127 t
ORIGIN      
        1 cgcttgcgcc atctgctcat tggtcaggaa ccagtcctgc tcgatgtcgg acggcgcgga
       61 cgagatcagc gagatcttgg tgtcggaatc gaggaacatc tccttcttgt aattgttgaa
      121 cttcaaatcc tcgatggtct ggtcgtgatc ggagagctgc ttgttccagc cggccttgcc
      181 gaccgccttg cgcatgtcga gaaaggtcgt gatgcgggtg tcgtcgcgca ggaaatgcgt
      241 gtgcatgtcc atgatgaact ggtctttcag cgcagcggcg cgctcttcag ccatcgcagg
      301 tgttgccgct tccgctttgg tcacgtcgaa cagcggtcca tagacttcgt tcatcgctac
      361 gaaggaagcg gccatgccgg ccgcggtctg gaagaatttc cggcggttca ggccctgctg
      421 gccgccgagc tcgtcggcca tcgcaagcag tcgcgcctcg acctcgcgtt gccgttcgtt
      481 ctgcgagtcg ggatagaatt catcgctgga gacgatctgg gtcgggattg gcgtctggta
      541 ttggcagagt tcggatggca tcaacgcggc aagttcttct tccgtcagat tgctgctcac
      601 ggctgttctc cctgattgcg ggtagccggt ttttccggct cgcgcggcgc gaaagactag
      661 ctctgctttt gcggcgagtc agccggtttc gcctgacatc gcgcaatggt gtctccgctg
      721 tgcagcgcac acttctttca aagctgcgcc cgcgctgcta gagtctattt atcggtcgaa
      781 aacttccaat ttgtcggtcg aagctacggc ttttgggaga tatcgcggga agcgaaatgg
      841 gaaacgacag ggcgaacacc atgactcatc tggaaaatcc gctggtacgc cgcgacgtca
      901 tcaagggcgc cggcctcggc cttattgccg gcacattcgc cggcgcgttg cccgcgcaag
      961 ctgcacccgt cgagggcagc gaagtctgga gcaacgaata ttgggccaag aagggcgacg
     1021 ttcccctgtg gatgtatcgc aagcgcctgg gcgcaccgaa ggcgggcgag ccggcgaagc
     1081 ccgtggtgtt tttcgtgcac ggctcctccg tcacctcgcg gtccttcgat ctcaaggtgc
     1141 caggcaaggg cgaatattcc gtcatgaacg agttcgcccg ctatggcttc gattgctgga
     1201 ccatggatca tgagaactac ggcaaatcgg gtcgtacctc gggcaattcg gatatcgcaa
     1261 gcggggtcga ggacctcaag gctgccgcag aggtcgtgac gcgcgagacc gggcaacaga
     1321 agtttcattt cataggcgaa tcctcaggcg ccttgcgcgc cggcgcctac gccatggtgg
     1381 cgccggaccg catcaaccgg ctggtgtttg ccgctttcac ctacaagggc gagggttcgc
     1441 cgacgctgaa gaagcgcgcc gaacagctcg actattatcg cacgcacaac atgcgcaagc
     1501 gcgaccgcga catgattcgt tcgatcgcga cccgcgacag gccggggacc agcgatgtcg
     1561 cagccgtcga ggcgctcgcc gatgtcgaac tgcagttcgg cgaccagatc ccgaccggta
     1621 cctatctcga catgaccgcc aatttgccgg tggtgcatcc ggagaaggta ttggcgccgg
     1681 tgctgctcgt gcgcggcgaa tatgacggga tcgccaccgt ccccgatctc gaggaattct
     1741 tcaacaagct gcccaacggc gaccggcagt tctccatcct gcccggcacc gcgcattcgg
     1801 tcgtgctggc gatcaaccgg caattgttct ggcatgtgac gcgtgcgttc ctgaacatgc
     1861 cgatgccgat cgcgacctga cgcaatcttc tcaggtgcct gctggcgcat cgccctggaa
     1921 cgaccgggga aattttgcaa ggcgttgtcg gagccacgtt cctgcgttcg tcctttgatg
     1981 atccaggacg acgaaaggaa ctcacttcat gtcaaaaatt tgcccctgcc tgtggttcga
     2041 cggcgaagcg gaagaggcgg cgacattcta cgtgtcgctg ttgcccggct ccgggatcga
     2101 gaccattcag aagaacagcg tcgacagccc cgcgggcaag gcgggctcgg tgctggttgt
     2161 ggaattcacg ctcgccggtc agcgcttcat ggcgctgaac ggaggcatgc ggatggaata
     2221 tacccacgcc gtctccttca agatcgactg cgcggaccag gccgaggtcg accgtctctg
     2281 ggacgcgctc accgcaaatg gcgggcaggc cgaacgttgc ggctggctcc gcgatcgtta
     2341 cggcgtctat tggcagatcg tgccttcggc gctgccgaaa tatctgggcg gagcggatcg
     2401 cgcaggcgca cagcgcgcaa tgcaggcgat gctcggaatg gtcaagctcg atatcgaagg
     2461 actaagacga gcctatgaag gcgcatgatg cccgcctagc cgcggcgcga taggttgctc
     2521 agccgccttc gtcggcacgt cgatgcagac cttccaccgc ttgataattt ttcaaagctg
     2581 gatctgaggg cggcgacggc gtatctgtcg tcgccgctcg ccgagctttc attgccagga
     2641 cgcgagcgag cgttccgaca ggtagatcat ctggcgtcca ccattcattg ccttgaggtt
     2701 gttgacgtag ttctcaggac gctcccagcg attgggaacc ttcaatccca tgaaatttgc
     2761 gatgctgccg ataaacgcat tgcaattgga cgtctcggcg ttccagaccg gcgaactcgc
     2821 ctgcaatttc ttgatgtatg cgaaaaccct ctttgcatcc ggctcgttca agtaaacccg
     2881 gtaactcgcg gtcaagtact gcggatcgag atcgccgtaa cttgccccgg tctcggacgg
     2941 cacccgcatc acatagccaa gtacataatc caggctgtct cccgcagggg taaggcccgc
     3001 gacttcaacc tgccgttcgc ctgtcctccc ataccagacg aaggcgtgtc cccaactcgc
     3061 cgccgttctg gcccggaaat cgacgtagta cgagcccttt gctgatcccg agcccggcgt
     3121 tcgagccgtg tgcgtcggtt tcgcttcgtc aagcgcatgc gcctcaaccg tgcgcttgtg
     3181 aacctcgtgt gcagaagctg attgaatttc gcacttgagc ataacagcgg ctgcgcaaaa
     3241 ccccacgagg aggcacggcg ccaacctgtt cagtcgattc gacatgcctt ctcccgtctg
     3301 gcagataccg agatccgagc cgatggctcg tatcagtagc tcctggttac gaccggtgac
     3361 ttgccgattg ggcttttgcc gacgggtgtc ttgccgatcg gcatcttgcc gacttgagcc
     3421 ggttgataga cgggctgtgg gtgcggcatg tcggcggcgc tggcgacggc caccatggca
     3481 agcgttgcac ctgttgcgat cagaattttc accaaggtcc ttctcctcaa catcaggagt
     3541 aacaagcaga gcgacctgca tgcgctccct tccaatgcgc ggcaaccatg aacctttgaa
     3601 ttcgtttaca ctgcggcaga agcatgaacc gcggagatcg ttttatgcgt caactacgac
     3661 acggtgcggc tgtttctaat cgcgatggaa cgatcacgcg gaacgccgga caaagtgaag
     3721 ccggaccctc ttggcaaatc gaattgaaaa gaaagacgag gatgcccggg catcgcggca
     3781 cgccgaagga gcttcggccc cacaggcggt caagcgggtg catcacgagt ccgtgtagca
     3841 accctcgacc tgagcgctag aagctgaacc aggaaatccg gctccagtag aagcggtggt
     3901 gatagagatg cggccagtag atcgggaatg ggtgccacca ggcatagcgc gagtgacggt
     3961 gcgcataatg ccggcgtcgg cgcggccttt catcgtcggt cacggccggt ttcgaaggct
     4021 ccgtcgtagt aaccggcttt acgggttcgg ttgtggcagc cggcttcgcg ggttcggtgg
     4081 tggccggcac gatcgagggc cgcggcacgc tgaccggcgc cggctggctt ggagttgctg
     4141 ccggtgtcgt ggtcggacca gcggccggtg ctggagcggc ggcatcctcg gcatggccgc
     4201 gaaaggtggg ggcagcgaca agaagcgctg cgacggcgaa acccataaca cgcgtcttca
     4261 tctcaatgat cccctttcct cgtttggtgg gcacggtccc ggactggctc gcgccaactt
     4321 gggaaacggc cggttcgctg atgcttcacg aaaatccgcc cgccgcggcg cggcaggcgg
     4381 aacgatagct gagaccgtca ctgcgcggcg gtcttggtgt ccatgttgac gacctgaacg
     4441 cgacggttga tcggatcggt cgggttctgc gcattcttca gcttggattt gccatagccg
     4501 acggtgacga gctcgccggt ggcgatgtga tagttgtcga ccaggtagcg cttgatggtg
     4561 tcggcgcggc gctcggacaa atcctggttg aacgcatcgc tgccgatcgc gtcggtgtgg
     4621 ccggcgacga cgaaggtcga gccgcgcaac ttcggatccg aaagcgcctg gccgagcgcc
     4681 tgcacggacg gcatcgagga atggctgatc gcggccgagt tgtagtcgaa ctggatttca
     4741 agatcgatct tcggcttggt cgcggcgatc tcggcgatct cctcgcgctc acccagcgag
     4801 agcgaccgcg tcctgcgatt gcggacgctg gcaaggaaat tcgcttcctt ggccttgacg
     4861 gccggatcga cctgcggggc gccgaccgac aggccgcggg tcaaaggctg cggcttcacg
     4921 tagagcgcgc caagaatttc gtccttggtg acgttgttgt ctgcggcaag cgccaggccc
     4981 gcggtcatcg agagcccggc gcaaatcgta atcgccttga gtccggcaaa cctgacgaag
     5041 ctggtcattg tcgtaatcct cgctgctatt cgccctttgg cgatttgatg ctgcgacgat
     5101 aggacaggtt cacacacttg aatgtgatgc atgtcacatg gcgaaaacgc cttcggaagc
     5161 aagcatttgg gccggccccg cggtgccgtg ggatcccagc caaggcgcct gttcgtcgtc
     5221 tgcgtgacct tcgatccgga tcaaataaat ttgcttcatc cggaccggca tcagcccaag
     5281 ccgccgcgtc gctccgagag acaaactgat ttgagggacc cggcgcgact gagtgaggcc
     5341 catcacagtc cgcgccagcg gtttggatca gattgtcccc caacggatgg acccgatacg
     5401 ggccaccgca cgggagaagg ggagaaatga tcatgcgctt ctggatcgca gcaatgtcag
     5461 tcgtagcgct tctcgccagc ggcaacgccg cactcgccga caagcgcgtc gccttcgtgg
     5521 tcggtaacgg cgcctacaag aacgtgccgc agcttcc
//
LOCUS       DATLJT010000560         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1871048,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000560 DATLJT010000000
VERSION     DATLJT010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871048"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM910_12940"
     CDS             <1..358
                     /locus_tag="VM910_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HVI63474.1"
                     /translation="NLFNLFGLIPFDPTQIPFFGHYLWLGVWPIIMGITMWFQMKLNP
                     TPPDPTQKIIFDWMPLIFTFMLAAFPAGLVIYWAWNNLLSVLQQSFIMRRNGVKVELF
                     DNLKATFLPKKAESKT"
     gene            482..1132
                     /gene="yihA"
                     /locus_tag="VM910_12945"
     CDS             482..1132
                     /gene="yihA"
                     /locus_tag="VM910_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVI63475.1"
                     /translation="MTADIDAKLVEAGRKLFAGDWRFAFASPSTETLPPMAGLEVAFA
                     GRSNVGKSSLINALTGRNNLARTSHTPGRTQELIFFQGPEKAGLRLVDMPGYGYAAAP
                     KTKVAAWTALIHAFLRGRSSLARVYVLIDARHGLKDVDLDVLKTLDSAAVSYQVVLTK
                     ADQVKPAELATRTAEAEAALAKHPAAFPQVLATSSRSGAGMAELRAAMALLLEERK"
     gene            1129..1503
                     /locus_tag="VM910_12950"
     CDS             1129..1503
                     /locus_tag="VM910_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HVI63476.1"
                     /translation="MSLRLARILVILAAIMGADGVVLAAASAHLADAARLGAASSMLL
                     FHATAVLGALVLSDRGTIDARIGLFAALGFVAGAGLFAGDLTLRQYTGHSLFPLAPPT
                     GGTLLILSWLLLAIAAAWPRKM"
     gene            1590..>2090
                     /gene="argB"
                     /locus_tag="VM910_12955"
     CDS             1590..>2090
                     /gene="argB"
                     /locus_tag="VM910_12955"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVI63477.1"
                     /translation="MNEAPNISPLDQARILSEALPHMQQYDDETIVIKYGGHAMGAED
                     TAKAFARDIVLLEQTAINPVVVHGGGPQIATMLKRLGIQSEFAAGLRITDAATIQIVE
                     MVLAGSINKQLVGYINEAGGKAVGLCGKDGNMVMASKATRTMVDPDSHIEKVVDLGFV
                     GEPEKVD"
BASE COUNT          380 a          678 c          640 g          392 t
ORIGIN      
        1 caatctgttc aacctgttcg ggctcattcc attcgatccg acccagatcc cgttcttcgg
       61 ccattatctg tggctcggcg tctggccgat catcatgggc atcacgatgt ggttccagat
      121 gaagctgaac ccgacgccgc ccgatccgac tcagaagatc atcttcgact ggatgcccct
      181 gatcttcacc ttcatgctgg cggccttccc cgcggggctc gtgatctact gggcctggaa
      241 caatttgttg tccgtgctgc agcagagctt catcatgcgc cgcaacggcg tgaaggtgga
      301 gttgtttgat aatctcaagg cgacattcct gcccaagaag gccgagtcca agacgtaagg
      361 ggcgcctctc tttcaaggat ttcgtcattg cgggcgaagc gaagcaatcc agagccgcgg
      421 caggactttc ggattgcgtc gtcgcttcgc tcctcgcagt gacgcgggtg agaaccttcg
      481 catgactgcc gatatcgatg cgaagctggt cgaagcaggc cgaaagctgt ttgccggcga
      541 ctggcgcttt gccttcgctt ccccctccac cgagacgctg ccgccgatgg ccggcctcga
      601 ggtcgcgttc gccggccgct ccaacgttgg caaatcctcg ctcatcaatg cgctcaccgg
      661 ccgcaacaat ctggcgcgga cctcgcacac gccgggccgc acccaagagc tgatcttctt
      721 ccagggtccg gaaaaggccg ggctgcggct ggtcgacatg cccggctacg gttacgccgc
      781 cgcgcccaag acaaaggtcg ccgcctggac cgcgctgatc cacgccttcc tgcgcggacg
      841 aagcagcctc gcgcgggtct atgtgctgat cgacgcgcgg catggactga aggacgtcga
      901 tctcgacgtg ctgaagacgc tggattccgc agcagtgagc tatcaggtcg tgctcaccaa
      961 agccgatcag gtcaaaccgg ccgagctggc aacgcgcact gcagaggccg aggcggcgct
     1021 ggcaaaacat cccgccgcct ttccacaagt cctcgcaaca tcgtcacgtt cgggcgccgg
     1081 gatggcggaa ttgcgcgctg cgatggcgct gctcttggag gagcgcaaat gagcctgcgc
     1141 cttgcgcgca tcctggtcat tctcgctgcg atcatgggcg ccgacggcgt cgtcctggcc
     1201 gcagcgtcgg cgcatctggc ggacgctgcg cggcttggcg cggcatcgtc catgctgctt
     1261 ttccacgcaa ccgccgtatt gggcgcgctg gtgctctccg atcgcggcac catcgacgcg
     1321 cgaatcggac ttttcgccgc cctcggcttt gtggcaggag ccggtctgtt cgcgggcgac
     1381 ctgaccttgc gccaatatac cgggcacagc cttttcccgc ttgccccgcc gaccggcggc
     1441 acactcctga ttctgagctg gctgttgctc gcgatagccg ccgcgtggcc gcgtaaaatg
     1501 tgacactttg cgcggtcggc gccaatcgga tagaacgcag cccaagtttg gctctgcccc
     1561 gccccgccgc cgaccacgag aagaagccca tgaacgaagc gccgaatatc agcccgctcg
     1621 atcaggcccg tatcctgtcc gaggcgctgc cgcacatgca gcaatatgac gatgagacca
     1681 tcgtcatcaa atatggcggc catgccatgg gcgcggagga caccgcgaaa gcgtttgcgc
     1741 gcgacatcgt gttgctggag cagaccgcaa tcaaccccgt cgtggtgcat ggcggcggac
     1801 cgcagatcgc gaccatgctg aaacggctcg ggattcaatc ggaattcgcg gcgggtctgc
     1861 gtatcaccga cgccgcgacc atccagatcg tcgaaatggt gctggcgggc tcgatcaaca
     1921 agcaactcgt cggctacatc aacgaggccg gcggcaaggc ggtcggcctg tgcggcaagg
     1981 acggcaacat ggtgatggcg tcgaaggcga cccgcaccat ggtcgatcct gactcgcata
     2041 tcgaaaaagt ggtcgatctc ggcttcgtcg gcgagccgga gaaggtcgat
//
LOCUS       DATLJT010000561         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1182543,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000561 DATLJT010000000
VERSION     DATLJT010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182543"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1635)
                     /locus_tag="VM910_12960"
     CDS             complement(106..1635)
                     /locus_tag="VM910_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63478.1"
                     /translation="MTPNPPEVEIVDFSKRFGAVQALDAVSVRFTAGRMHALLGENGA
                     GKSTLVKCLMGYYKVDAGRILVDRQARAIAHPYDAHALGLGMVYQHFTLVPQMSVAEN
                     FVLGRERLPAVLDWAAEHTALRKFMARMPFRLDPRRLVATLAAGEKQKLEILKQLYLG
                     RQFLVLDEPTSVLTPGEADEVLGEMRRLADAGELTVVLITHKLREVMEFAQDVTILRG
                     GKVVARIDAADASEQRLAAMMFSQSHDTAKLELASRTGPSANYLEIEHLHALGDRDTI
                     AVAGASLAVRSGEIVGIAGVSGNGQKELVEVLAGQREAESGSLRIAGRAFNGTRRELK
                     QYGVSLLTEEPLANAAVRSMSVMENLSLRQFDQPPLARGRFVLNRRAMRPFAEDLIRR
                     YGIRTASLFARLDTLSGGNIQRTVLARELSRDVRLLIMQNPCVGLDAAAVAEIRSQIM
                     RARERGAAILLISEDLDEILDLSDRVLVMAAGRISHETDRAGADRYDIGRHMAQGRLT
                     A"
     gene            complement(1632..2561)
                     /locus_tag="VM910_12965"
     CDS             complement(1632..2561)
                     /locus_tag="VM910_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63479.1"
                     /translation="MSSTLDLGWWGVVLAIIAGAIRVSTPYLLVSLGETLTEKSGRVN
                     LGLEGTLVLGAMSGFATSYLTGSPWLGLLAAGVSGAAMGLLHALICNLPRVNDIAVGI
                     ALFIFGIGLAFYLGKPFIEPRAPMLPALPLGSWSHHPAIRSAFDVSPLFFIGIALALA
                     LEWGMKNTRWGLIVRMAGDSADAARAIRRSVERIRTYATIAGGFLSGVGGGFLALFYP
                     GNWNEGLSSGQGLMAVALVIFARWSPVRCFYASLLFGGASAIGPAVQTVGITEGYYLF
                     YAAPYALTLALLIYSSSPTQALAGAPNELSISR"
     gene            complement(2558..>2927)
                     /locus_tag="VM910_12970"
     CDS             complement(2558..>2927)
                     /locus_tag="VM910_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63480.1"
                     /translation="NRRAAQMAGLPIGRLMLLACFLGGAAAGIAGAIEIVAVHGAASE
                     SLVVGLGYTGILVAFLARQNALAVLLVALLLGGISASGGLLQRRFDMPHAVTSVLQGL
                     IFICVLASNASAGRWRRSSG"
BASE COUNT          561 a          976 c          926 g          464 t
ORIGIN      
        1 ccaccggctc gatcgctcgg atgaaaccgt gctcgatgag cacgtccgct ggatcgcacg
       61 tcacaaaatc cgcgcgcagc acacgcccgc cgcgaatcaa ggtgctcatg cggtcagcct
      121 gccctgcgcc atgtgtcgtc caatatcgta gcgatcggcg cctgcccgat ccgtctcgtg
      181 gctgatgcgg cctgccgcca tcaccagcac acggtcggaa aggtcaagaa tctcatcaag
      241 gtcttcgctg ataagcagga tcgcggcacc gcgttctcgc gcccgcatga tctgactgcg
      301 gatctcggcg accgccgccg catccagtcc gacgcaggga ttctgcatga tcaagagccg
      361 cacgtcccgc gacagttcgc gtgccagcac ggtgcgctgg atattgccgc cggacagcgt
      421 atcgagtcgc gcgaacagcg atgcggtccg gatgccgtag cgccggatca ggtcttcggc
      481 gaacggtcgc atggcgcggc ggttgaggac gaaccggccg cgggccagcg gcggctggtc
      541 gaactggcgg agggaaagat tttccatcac cgacatggag cggaccgcgg cgttggcaag
      601 tggctcctcg gtcagcaggg agactccgta ttgcttaagc tcgcgccgcg taccgttgaa
      661 tgcgcgcccc gcgatgcgca ggcttccgct ctcagcctcg cgctgtcctg ccagaacttc
      721 aacgagctcc ttctgcccgt tgccggatac gccggcgatg ccgacgatct cgccagagcg
      781 gaccgcgagg ctcgcaccgg cgaccgcgat cgtgtcgcga tcgcctagcg catgcaggtg
      841 ctcgatctcg agatagttgg cggagggccc ggtgcgcgat gcgagctcca gttttgccgt
      901 gtcgtgcgac tggctgaaca tcatcgcagc caggcgctgt tcagaggcgt ccgccgcatc
      961 gatccgcgcc accacttttc cgccccgcag gatcgtcacg tcctgagcaa attccatcac
     1021 ctcgcgcagc ttgtgggtga tcaggacgac ggtgagttct ccagcatcgg ccaggcggcg
     1081 catttcgccc agcacttcgt ccgcctcgcc cggggtgagg accgaggtcg gttcatcgag
     1141 tacgagaaat tgccggccga gatagagctg tttcagaatc tccagttttt gcttctcgcc
     1201 ggcggcaagc gtcgccacca gccggcgcgg atcgagccgg aatggcatgc gcgccatgaa
     1261 cttccgcaaa gcggtgtgct cggctgccca atcgaggact gctggcaatc gctcgcggcc
     1321 gagcacgaaa ttctccgcca cgctcatctg cggcaccagc gtgaaatgct ggtacaccat
     1381 gccgagaccg agcgcgtggg cgtcgtacgg atgggcaatc gcgcgggcct gccggtcaac
     1441 gagaatgcgg ccagcgtcga ccttgtaata tcccatcagg cacttcacca gcgtgctctt
     1501 gccggcgccg ttctcgccca gcagggcgtg catgcgccca gcggtaaatc gcaccgatac
     1561 agcgtccagt gcttgcacag caccgaaccg cttggaaaaa tcgacgatct cgacctcagg
     1621 cgggttcggc gtcatctcga tatcgacaat tcgttggggg cgccagcaag cgcctgtgtg
     1681 ggcgaggaac tgtagatcag caaggcgagc gtcaacgcgt agggtgcggc atagaagaga
     1741 taatagccct cggtgatccc gacggtctgc accgccggac caatcgccga agctcctccg
     1801 aacaggagcg aggcgtagaa acagcgaacc ggtgaccacc gcgcgaagat gaccagcgcc
     1861 accgccatca ggccttggcc gctcgacagg ccttcgttcc agttgcccgg atagaagagc
     1921 gcaagaaagc cgccgccaac accggagaga aagccgcccg ctatggtggc ataagtgcgg
     1981 atgcgctcga ccgatcgcct gatggcgcgc gccgcatcgg cgctatcccc ggccatgcgc
     2041 acaatgagcc cccagcgggt attcttcatc ccccattcga gcgcgagggc gagcgcaatc
     2101 ccgatgaaga acagtgggct cacgtcgaag gcggagcgga tcgcgggatg gtggctccag
     2161 ctgcccaggg ggagtgccgg cagcatcggt gcccgcggtt cgatgaacgg cttgccgaga
     2221 tagaacgcta gcccgatccc gaaaatgaac aatgcgatcc caacggcgat gtcgttcacg
     2281 cgcgggaggt tgcagatcag cgcgtgcaga agtcccatcg cagcgcccga aacaccggct
     2341 gccagtagac ccagccaagg cgatccggtc agatacgagg tggcgaaccc gctcatggcg
     2401 ccgagcacca gcgtcccctc gagcccgaga ttcactcgcc cgctcttttc cgtgagcgtc
     2461 tcgccgaggc tcaccagcag atagggcgtg gagacgcgaa tggcgccagc gatgatggcc
     2521 aggaccacgc cccaccatcc gagatcgagc gtgctgctca tcccgagctc cgacgccagc
     2581 gcccggccga cgcattgctg gccagcacgc agatgaagat caggccctgc aacacgctgg
     2641 tgaccgcatg cggcatgtcg aacctgcgtt gtaacagccc gccgctcgcc gagatgccgc
     2701 cgagcaggag tgccaccagc aggacggcca gtgcattctg ccgcgccagg aatgcgacca
     2761 ggatcccggt gtagcccaag ccgacgacga gcgattcgct ggcggcgccg tgcaccgcga
     2821 cgatctcgat ggcgcccgca atcccagcgg cggcgccgcc caggaagcat gccagcagca
     2881 taagccggcc gatcggtaag cccgccatct gcgcagcgcg ccggttg
//
LOCUS       DATLJT010000562         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_722046,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000562 DATLJT010000000
VERSION     DATLJT010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722046"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1157
                     /locus_tag="VM910_12975"
     CDS             12..1157
                     /locus_tag="VM910_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HVI63481.1"
                     /translation="MLFFDNTIQTATLRTWTIGFTVSQTLFNGFKTANTVRQSESQVL
                     SGREALRNTGQGVLLDAVTAYTNVIANQSLVEAQRINVTFLRETLATTKTRLEGGDVT
                     PTDVAQAEARLSRGLADLNAAEVGLAISQALYAQVIGVPPGRLVPAESIDRLLPRTRE
                     DALAISRKEHPAILAAMYDIDVAQFGTKIAESSLWPTLSVQGSLQRQWENDPTLSAKG
                     NDIASFLGTANVPLYDGGLAASQIRQAKEIAMQARIVLELIRNQTQTAVLSAWVTFEG
                     AKVALTAAEAEVRAATIALAGVQKEAQAGQRTTLEVLNSQQDLTAARARLIGAQRDRV
                     IAAYALLGAAGRLDVKTLGLHTPDYAPEVHYHQVRDAWHGLRTPAGQ"
     gene            1453..1665
                     /locus_tag="VM910_12980"
     CDS             1453..1665
                     /locus_tag="VM910_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63482.1"
                     /translation="MWLIVYYVIFMIAGDLAAYVIGLLTEREFGGQVSLIVFLALYFL
                     FLWVSWVLAVWMTEPKHAEPAGTESV"
     gene            1775..2002
                     /locus_tag="VM910_12985"
     CDS             1775..2002
                     /locus_tag="VM910_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63483.1"
                     /translation="MPDGSAQSIATGLAGGLIVIALLETLLDKNVIALAEARHILQRA
                     VNSLSPARTTEEREALNILAGMQRGYFSERR"
     gene            complement(2113..>3402)
                     /locus_tag="VM910_12990"
     CDS             complement(2113..>3402)
                     /locus_tag="VM910_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI63484.1"
                     /translation="AIFMPLVLYYFFKWAANDLHDQAMREQADLVAHYLVLRPDGSWT
                     LDLPPALQDLYSQAVGRYAYAVLDDTGRVLFSSLKDRSLIFSTDPRSSDVAYLETRRG
                     TAAVSGVSLVKEADGRKVWVQVGEDLTHRDVIIDDIVADFFKRVGWITLPMLLFLLAI
                     DIVIFRRALRPLINASEMAKKINPKRTDVRLPPEEIPKEILPLVQAVNQALDRLEAGF
                     RVQREFTADAAHELRTPLTILRSRVDTLADRGISKALHQDIEGMARIVSQLLDIAELD
                     AFSIDPLEKADLRAICAEVAEFAAPLALARGKNIALSGSDAPVWVNGNPEMLSRAIRN
                     LVENAINYSPPGTTVEIVVEGSGMVRVLDEGPGIKEDERELIFQRFWRRDRRRTGNAG
                     LGLSIVQRIADTHAATVSVENRPTGGANFSLSFARVG"
BASE COUNT          662 a         1082 c         1040 g          618 t
ORIGIN      
        1 agcaggtgcg agtgctgttt ttcgacaata ccattcagac ggcgacgctg cgcacctgga
       61 ccatcgggtt caccgtatcg cagaccctgt tcaacggctt taagaccgcg aacaccgttc
      121 gtcagtcgga atcgcaggtg ctctcggggc gcgaggcgtt gcgcaatacc ggccaaggcg
      181 tgctgctcga cgcggtgacc gcctatacca acgtgatcgc taaccagagc ctggtcgagg
      241 cccaacgcat caatgtcacg tttctgcgcg agacgctcgc caccacgaag acgcgtctcg
      301 aaggcggcga cgtgacgccg acggacgtgg cgcaggcgga ggcacggctg agccgcgggc
      361 tcgccgatct caacgccgcg gaggtcgggc tggccatcag ccaggcgctt tacgcccagg
      421 tcatcggcgt gccgcccggc cggctggtgc cggcggaatc gatcgaccgg ctgctgccgc
      481 gcacgcgcga ggacgcgctc gcgatcagcc gcaaggagca tcccgccatt ctggcggcga
      541 tgtacgacat cgatgtcgcc caattcggca ccaagatcgc cgagagcagc ttgtggccga
      601 ccctctcggt gcagggaagt ctccagcgcc aatgggagaa cgatcctacg ctcagcgcca
      661 agggcaacga tatcgcctcg ttcctgggaa cggccaacgt gccgctttac gacggcggat
      721 tggcggcctc gcagatccgg caggccaagg agatcgcgat gcaggcccgc atcgtgctgg
      781 aactcatccg caatcagacc cagaccgcgg ttctgagcgc ctgggtcacc ttcgaggggg
      841 cgaaggttgc gctcaccgcc gccgaagcgg aggtgcgcgc cgccactatc gcgctcgccg
      901 gcgtgcagaa ggaagcgcag gcgggccagc gcacgacgct tgaggtcctc aactcgcagc
      961 aggacctcac ggccgcgcgc gcgcggctga tcggcgcgca acgggaccgc gtgatcgccg
     1021 cctatgcgct gctcggcgcc gccggacggc tcgacgtcaa gacgctcggc ctccacaccc
     1081 cggactatgc gcccgaggtc cactaccatc aggtgcgcga tgcctggcac ggcctgcgca
     1141 cgccggcggg gcagtaggtc aaccgcaaac atgccgtagg aatggatgga tctcgctccg
     1201 ccaggcgaga ccttccgggc acagatcccc tcctccacac cgagatcgct gggcacgtcg
     1261 gagcgagcca gagacctgtc ggacacacgc gccgggtttt tttgcttttc cgaccgcgcc
     1321 gtcaggtctg tgtaaggaaa atattgaaga gtgccggtcg cgcgccgaag tgcggtcgtc
     1381 ccgccgagct ctatcttgcc gcgtcggttg ggcgttcccc gccaaggcag tcggcatcag
     1441 ggggaatttg tcgtgtggct catcgtctac tacgtcatct tcatgatcgc gggcgatctc
     1501 gccgcctatg tcatcggcct cctcaccgaa cgcgagtttg gcggccaggt cagcctcatc
     1561 gtctttctcg cgctttattt tcttttctta tgggtttctt gggtgcttgc ggtatggatg
     1621 acagagccca agcacgccga accggcgggt accgagtcgg tctgatcgtg ggaaaggaaa
     1681 gccagctgcg acgaggtcgc aggcgcaggt tcgacgatgc gaggcgctat aggtagacta
     1741 gcttcggcat taatgggagg aatgggaggt ccccatgccc gacggaagcg cccaaagcat
     1801 tgcaacggga ctagcgggcg gcctgatcgt catcgccctg cttgaaactc tactggacaa
     1861 gaacgtgatc gccttggcgg aggccagaca catcctgcaa cgcgcggtca actcgctgag
     1921 cccggccagg accacggagg aacgggaagc tctcaacatc ctcgccggaa tgcaacgcgg
     1981 ctacttttcg gaacgccgct gacaagaacc catggcgacc gcggcgcggt tctcaacatc
     2041 ttggcggcgc ttatcccgcg atatctcggg cgctgccaga cctgacgtct gcgccgacag
     2101 ccgcgcccag tgttaaccaa ctcgagcaaa gctcagcgaa aagtttgcgc cgccggtcgg
     2161 gcggttctcg acgctgacgg tcgcggcatg ggtatcggcg atgcgctgga cgattgaaag
     2221 tcccaacccc gcgtttcccg tccgtcgccg gtcgcgccgc cagaagcgtt gaaaaatcag
     2281 ctcgcgctca tcctctttga tgcccggacc ctcatcgaga acccgcacca tgccgctccc
     2341 ctccacgacg atctccacgg tcgtccccgg cggagaatag ttgatggcgt tctcgaccag
     2401 attacggatc gctcgtgaca gcatttccgg attgccgttg acccatacag gtgcgtcgct
     2461 gccgctcaag gcgatatttt tgcctctcgc cagcgccaac ggagcggcga actccgcgac
     2521 ctcggcacaa atcgctcgca gatcggcttt ctccaacgga tcgatcgaga acgcgtccaa
     2581 ttccgcgatg tcgagcaatt ggctgacgat acgcgccatg ccttcgatat cttgatgcag
     2641 cgctttggaa attcctcgat cggccagggt gtcgacgcgg ctgcgcaaga tcgtcagcgg
     2701 cgtgcgcaac tcatgcgccg catcggcggt gaactcccgc tgaacgcgga atccggcctc
     2761 gagtcgatca agcgcttggt tgacagcttg cacgaggggc aaaatttctt tgggaatttc
     2821 ttcgggcggc agacgtacgt ccgttctctt cggattgatt tttttcgcca tttccgacgc
     2881 gttaattagc ggccgcaacg ccctacggaa aatcacgatg tcgatcgcca acaagaataa
     2941 gagcatcggt agcgtgatcc atcccacgcg tttgaaaaaa tcggcaacga tatcgtcgat
     3001 tatgacatcc cgatgcgtca gatcctctcc gacctgaacc cataccttgc ggccatccgc
     3061 ctccttcacg agactgacgc cggagacggc tgcggtgccg cgtcgtgtct ctaggtaggc
     3121 gacatcggag gaacgcggat ccgtggaaaa tatcagcgac cggtctttga gcgaggagaa
     3181 caagactcgt ccggtatcgt cgaggaccgc ataggcatac cgtccgaccg cctgtgaata
     3241 gaggtcctga agcgcgggcg gcagatcgag cgtccagctg ccatccggcc gcaacacgag
     3301 ataatgggca accaagtcgg cctgctcacg catggcttga tcgtgaaggt cgttcgccgc
     3361 ccatttgaag aaataataga gtaccaacgg catgaagatc gc
//
LOCUS       DATLJT010000563         1667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2791298,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000563 DATLJT010000000
VERSION     DATLJT010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791298"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1356
                     /gene="oxlT"
                     /locus_tag="VM910_12995"
     CDS             82..1356
                     /gene="oxlT"
                     /locus_tag="VM910_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HVI63485.1"
                     /translation="MTTETISSRAIASESTRWGQLIFGIICMVMIANLQYGWTLFVNP
                     IDQKYHWGRAAIQVAFTIFVLTETWLVPIEGYLIDRFGPRIMISGSGVLVAIAWVINS
                     VADSLPLLYLGAAIGGIGAGVIYGGSVGNALKWFPDRRGLAAGLTAAGFGAGSALTVI
                     PIANMIQSSGYQAAFLWFGLGQGIVVVLVALLLRAPQPGEVPVPAAPVVQQTRRDYGP
                     MEVLSSMPFWVMYVMFVMVGTGGLMATAQLAPIAKDFKIDTIPVSILGLTLPALQFAL
                     SIDRVLNGITRPFFGWVSDQIGRENTMFIAFMAEGIGIYALLHFATDPLLFVILSGVV
                     FFAWGEIYSLFPATCTDIYGRKYATTNYGLLYTAKGTAALLVPLGNVLTTWTGSWTAV
                     FVIAAILNIIAAVMALVVLRPIRQRAMAMAMA"
     gene            complement(1448..>1667)
                     /locus_tag="VM910_13000"
     CDS             complement(1448..>1667)
                     /locus_tag="VM910_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63486.1"
                     /translation="FTGTRREEALGHWCETEEEPMSDLAARFVYAPLLSTGLKVVCLF
                     CVFGLALSAVVIPLIAPEYLTWVLAHIE"
BASE COUNT          286 a          517 c          520 g          344 t
ORIGIN      
        1 aggcattgtt gatcgcgttg cgattaacgg ctgcgatgcg aagcgtgccc ctcgatcaat
       61 aaggctggga tggaggaaac ggtgacgaca gaaacgattt cgtcgcgggc gatagcctcg
      121 gagtccacgc gttggggcca gttgattttc ggcatcatct gcatggtgat gatcgccaac
      181 ctgcaatatg gctggacgct cttcgtcaat cccatcgatc agaaatacca ttgggggcgc
      241 gcggcgatcc aagtcgcgtt taccatcttc gtcttgacgg agacgtggct ggtgccgatc
      301 gaaggctacc tgatcgatag attcggcccg cggatcatga tcagtggcag cggcgtcctc
      361 gtcgccattg cctgggtcat caactcggtg gccgattcac tccctctcct ttacctcggc
      421 gcggcgatcg gcggcattgg tgccggcgtc atctacggcg gctcggtcgg caatgcgctc
      481 aagtggttcc cggatcgccg cggcctcgcg gccggcttga ccgccgccgg tttcggcgcc
      541 ggctccgccc tgaccgtgat cccgattgcg aacatgatcc agtcgagcgg ctaccaagcc
      601 gcattcctct ggttcgggct cggacagggc atcgtcgtgg tgctggtggc gctgctgttg
      661 cgggcaccgc agccgggcga agtgccggtg ccggcggcgc ccgtcgtcca gcagacccga
      721 cgcgactacg gtcccatgga ggtgctcagc tcgatgccat tctgggtcat gtatgtcatg
      781 ttcgtcatgg tcggcaccgg cggcctgatg gcgacggcgc agctcgcgcc cattgccaag
      841 gacttcaaga tcgacacgat cccggtgtcg attctcggac tgacactacc tgcgctgcaa
      901 ttcgcgctct cgatcgatcg cgtgctcaac ggcatcactc ggcccttctt cggctgggtc
      961 tccgaccaga tcggtcgtga gaacacgatg ttcatcgcct tcatggccga gggcatcggc
     1021 atctatgcgc tgttgcattt cgccaccgat ccgctcctgt tcgtgatcct gtccggggtg
     1081 gtgttcttcg cctggggcga gatctattcg ctgttcccgg cgacatgcac cgacatttat
     1141 ggccgcaaat acgccaccac gaactacggc ttgttgtaca cggcgaaagg gacggccgcg
     1201 ctcctggttc cgcttggcaa cgtgctgacg acctggaccg gcagttggac ggcggttttc
     1261 gtgatcgccg cgattctcaa cattatcgcc gcggtcatgg cgctggtcgt gctgcggccg
     1321 atacggcaac gcgcaatggc aatggcaatg gcttgaggac ggcgtccaac ggcgagcgtc
     1381 ggcgcggtta gcggcgctca ccgtcttaca agttgtcgcc cggcgaatga ggccctgacg
     1441 tggagcctca ttcgatgtgg gccagcaccc aggtcagata ctccggcgcg atcaacggaa
     1501 tgaccactgc cgagagcgcc agaccaaaca cgcagaacag gcacacgacc ttcaagccgg
     1561 ttgaaaggag cggtgcgtag acaaagcggg cggcaagatc ggacatgggc tcctcctcgg
     1621 tctcgcacca atgtcctagc gcttcctccc gcctcgtccc tgtgaag
//
LOCUS       DATLJT010000564         3988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3020486,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000564 DATLJT010000000
VERSION     DATLJT010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020486"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="VM910_13005"
     CDS             complement(<1..1773)
                     /locus_tag="VM910_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI63487.1"
                     /translation="MLIKRTQHRSETASRGSIAGSLSGQSDGLDRRTFLRRSGIAGGA
                     LAALGTLPIGNVRKAKAAMAGPLTSGATVRKSICTHCSVGCTVTAEVLNGVWIGQEPS
                     WDSPINRGSHCAKGASVRELVHSERRLRYPMKLVGGQWTRVSWDTAINEIGDKLLEVR
                     EKSGPDSVYWLGSAKMTNEGAYLFRKLGAFWGTNNTDHQARICHSTTVTGVANTWGYG
                     AMTNSFNDIRNAKTQVIMGGNPAEAHPVSLQHLLEGKELQKANFIVIDPRLTRTAAHA
                     TEYVRMRPGTDIPVLYGMMWHILQNGWEDKEFIKQRVYGFDDLRKEVEKWNPEEVERV
                     SGVPGEQLKRVAKMLATEKPATLIWAMGQTQKTVGTANVRASCIALLMTGNVGNPGTG
                     ANIFRGHDNVQGATDVGLDIVTLPFYYGLAEGAWKHWARVWEVDYEFLKSRFDSKQIM
                     ETPGIPLTRWFDAVTLPKDRVAQKDNVRAVFVQGHASNSITRVPESLNGLKALELLVI
                     ADPHPTTWASLSVEAGRKDGVYILPVATQFECKGSRVASNRSLQWGEQIVKPVFESKD
                     DLEVIYLMAKKCGFADRMFKNIKVE"
     gene            complement(1784..1993)
                     /locus_tag="VM910_13010"
     CDS             complement(1784..1993)
                     /locus_tag="VM910_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI63488.1"
                     /translation="MRDEKKTTGGRRDFLRKVGIGAVGAGATLATPLITPAQADSESD
                     QEKRKARYKETDHVKTYYRVNRYPG"
     gene            complement(2022..2801)
                     /locus_tag="VM910_13015"
     CDS             complement(2022..2801)
                     /locus_tag="VM910_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HVI63489.1"
                     /translation="MRDAGLERAINAAGGVAQLARKIGIAQPSVSNWNTVPAQRVIAV
                     EAATGVSRKVLRPDLYSERIVPDGAIDVIDAARAQEYALLANLLLSAPSAALLEQIAQ
                     LNADATPLGRAHAALAEAASIAVATEVEREYFDLFVGLGRGELLPYASYYLTGFLNER
                     PLSRLRDDLAALGIERMENNFEPEDHAATLCEIMAGLAAARFPASPQAQRAFFEKHVS
                     RWMGRLFADMEKAENAKFYRSVGTLGRVFLEIESEAFTFAN"
     gene            complement(2922..3578)
                     /locus_tag="VM910_13020"
     CDS             complement(2922..3578)
                     /locus_tag="VM910_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HVI63490.1"
                     /translation="MSDEQFLARWWRCKQEAKADHAERAPEKGAEAHESEPSNRAAAE
                     PVVSEAELSNLPPIESIDANTDITAFLRKGIPQEVSRAALRRAWIADPAIRDFVGLAE
                     NAWDFNDPNAMAGFGALDYSAEQIDTLVRRIVHGVLKTADSPPNPLGETAEDAARSAH
                     AEPGAQSSNAGKATTDPRPSDVPAELPSKPAASQPAAASVASEETFLLRRTHGGALPQ
                     "
     gene            complement(3575..>3988)
                     /locus_tag="VM910_13025"
     CDS             complement(3575..>3988)
                     /locus_tag="VM910_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HVI63491.1"
                     /translation="GVLPDEPETTPWTVIREQEETTLFYAGGATVDLYRSETERYRDN
                     LATGAPSVWIVLTPSEGAWPYAVSAVTADPAEGEAFTEAGTNLVEAVPMPEMLHQAIE
                     NFVAEHHVETEFVKRERRRADPEVLARRHHEGGHE"
BASE COUNT          715 a         1240 c         1210 g          823 t
ORIGIN      
        1 ctcgaccttg atgttcttga acatcctgtc ggcaaagccg cacttcttcg ccatcagata
       61 aatgacttcg aggtcatctt tcgattcgaa caccggcttt acgatctgct cgccccactg
      121 cagcgagcgg ttcgaggcga cgcgcgaacc cttgcactcg aattgcgtgg caaccgggag
      181 aatgtagacg ccgtccttgc gtcccgcctc caccgagagc gatgcccagg tggtcggatg
      241 cgggtcggcg atgacaagca gttcgagcgc cttcagacca ttgagggatt caggaacgcg
      301 cgtgatgcta ttgctggcgt gcccctgcac gaaaaccgcc cgcacgttgt ctttctgcgc
      361 gacccgatct ttcggcagtg tcaccgcgtc gaaccagcgt gtgagcggga tgccgggggt
      421 ttccatgatc tgtttggaat cgaagcgcga tttcaggaac tcatagtcga cttcccaaac
      481 ccgcgcccag tgcttccacg cgccctcggc gaggccgtag tagaatggca gcgtgacaat
      541 atcgagcccg acgtcggtcg cgccttgaac attgtcatgg ccacggaaga tattggcgcc
      601 cgtgccgggg ttgccgacat tgccggtcat cagcagcgcg atgcaactcg cccgtacgtt
      661 tgcggtgccg acggtcttct gggtctgccc catggcccag atcagcgttg ccggcttctc
      721 cgtggcaagc atcttggcga cgcgcttgag ctgttcgcca ggaacgccgc tgacgcgctc
      781 gacctcttcc ggattccact tctccacttc cttgcggaga tcatcgaaac cgtagacgcg
      841 ctgtttgatg aactccttgt cctcccaccc gttctggaga atgtgccaca tcatgccgta
      901 gagcaccggg atatcggtgc caggtcgcat gcgcacatat tcggtcgcat gggcggcggt
      961 acgcgtcagg cgcggatcaa tgacgataaa gttggccttt tgaagttcct tgccctcgag
     1021 cagatgctga agcgataccg gatgcgcctc ggccggattg ccgcccatga tgacctgggt
     1081 tttcgcattg cggatgtcgt tgaagctgtt ggtcatcgca ccgtagcccc aggtgttggc
     1141 tacgccggtg acggtcgttg aatggcaaat gcgcgcctga tggtcggtgt tgttggtacc
     1201 ccagaacgcg ccgagcttgc ggaacagata ggcaccctcg ttcgtcatct tggccgatcc
     1261 aagccagtag actgaatcgg ggccggactt ctcgcgaact tcgagcagct tgtcgccgat
     1321 ctcgttgatc gcggtgtccc aggagacacg cgtccactgt ccgcccacca gcttcatcgg
     1381 ataacggagc cggcgttcgc tgtgcacgag ctcgcgcacg gaagcgcctt tcgcacaatg
     1441 cgatccgcga ttgatcggcg aatcccaact cggctcttgg ccgatccata ccccgttcag
     1501 cacctctgcg gtcacggtgc atccaaccga gcaatgtgtg caaatgctct tgcgaacggt
     1561 cgctcctgag gtgagtggac cggccatggc cgctttcgcc ttgcggacgt tgccgatcgg
     1621 cagcgtgccc agagcggcga gagcgccgcc ggcaatgccg gaccggcgca ggaaggtgcg
     1681 gcggtcgaga ccgtcgcttt gcccggacag ggatcctgcg attgatccgc gtgaagcggt
     1741 ttcggaacgg tgttgtgttc tcttgatcag cacggccgcc tcctcaacca ggataacgat
     1801 tgacgcgata ataggttttc acgtggtcgg tttccttgta gcgcgccttg cgtttctctt
     1861 ggtcgctctc gctgtccgct tgcgcgggcg tgatcaaggg tgttgccagc gtcgcacccg
     1921 ctccgaccgc gccgatgccg accttgcgga ggaaatcgcg gcggccgcct gtcgttttct
     1981 tctcgtctct catcgcgtct ctcctggacc agcggcgcca gtcagttggc gaacgtgaaa
     2041 gcttcggatt ctatttccag aaaaactcgt ccaagtgttc cgaccgaccg gtagaatttg
     2101 gcgttctccg ccttctccat gtctgcgaac aaacgtccca tccagcggga cacatgcttc
     2161 tcgaagaagg cgcgctgtgc ctgcggcgat gccgggaagc gcgccgccgc aaggcccgcc
     2221 ataatctcgc acagcgtcgc cgcgtgatcc tcgggctcaa aattgttctc catccgctcg
     2281 atgccgagcg cggcaagatc gtcgcggagg cgtgacagcg ggcgttcgtt gagaaatccg
     2341 gtcaggtaat acgatgcgta tgggagcaac tcgccacggc cgagaccgac gaacagatcg
     2401 aaatattcgc gctcgacctc ggttgcaacc gcaatcgatg cggcctctgc aagagctgcg
     2461 tgggcgcgcc caagcggggt cgcatcagca ttcagttgcg cgatctgctc gagcaatgcg
     2521 gccgacggcg cggacaaaag caagtttgca agcagcgcgt attcctgagc gcgcgcggca
     2581 tcgatcacat caatcgcgcc gtccggcacg atgcgctcgc tatagagatc aggacgaagc
     2641 accttgcgag acacgccggt cgcggcctcg accgcaatca cacgttgcgc gggcacggtg
     2701 ttccagttcg acaccgaggg ctgggcaata ccaatctttc gggcaagctg ggcaacgccg
     2761 cccgccgcat tgatcgcacg ttcaaggccc gcatcgcgca cgccgacctc ccatcctaag
     2821 gggcgaagcg cttgattgcg tttgcttaat aaaaagtagg cgcgtattgg gtagcggtca
     2881 attaacagcc atagcttgag gctatcgcat ttcgtcgcag tctactgagg caacgctccg
     2941 ccatgtgtgc ggcgcagaag gaaagtctcc tcactcgcga cactcgcagc tgccggttgc
     3001 gatgcagcag gctttgaggg caattccgcg ggcacgtcgg acggtcgagg atctgtggtc
     3061 gccttgcccg cgttcgatga ttgcgcgccg ggctccgcat gagccgatcg cgccgcgtct
     3121 tcggcggtct cgccgagtgg gttgggggga ctgtcagcgg tcttcaacac gccgtggacg
     3181 atgcggcgga cgagtgtgtc aatctgctcg gctgaatagt ccagcgcgcc gaagccagcc
     3241 atggcgttcg ggtcattgaa gtcccaggcg ttttccgcca accccacgaa atcgcggatc
     3301 gcgggatccg cgatccaggc gcgacgaagc gccgcacggc tcacctcctg cggaatgcct
     3361 ttgcgcagga aggccgtgat gtcggtatta gcatcaatcg attcaattgg gggcaggttc
     3421 gaaagttccg cctcggagac aactggctct gctgcagcac ggttggacgg ctccgactca
     3481 tgcgcctcag cacctttttc gggtgctcgt tcagcgtgat cggccttcgc ctcttgcttg
     3541 cagcgccacc agcgcgccag gaattgctcg tcgctcattc gtgcccacct tcgtggtgac
     3601 gccgggcaag cacctctgga tcggcgcggc gccgctcgcg cttgacgaat tccgtctcga
     3661 cgtggtgctc ggcgacgaaa ttttcaatcg cctgatgcag catctcaggc atcggcacgg
     3721 cctcgaccag attggtaccg gcttccgtga atgcctcccc ttccgcgggg tccgcggtga
     3781 cggccgacac agcatagggc caggcgccct cggaaggagt caatacgatc cagacactgg
     3841 gagcgccggt ggcaagattg tcgcggtagc gctcggtttc ggaacgatag agatcaacag
     3901 tcgcgccccc ggcataaaag agtgtcgttt cctcctgctc ccgtatgacg gtccacggcg
     3961 tcgtttccgg ttcatccggt aacacgcc
//
LOCUS       DATLJT010000565         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2791460,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000565 DATLJT010000000
VERSION     DATLJT010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791460"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VM910_13030"
     CDS             complement(<1..1434)
                     /locus_tag="VM910_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI63492.1"
                     /translation="MSKNFGTIVVCDFEYEVASGSMPNVLCMVAYVLDEQLEHVHTVR
                     LWRGDFGSVPPFDTGPDALFVAYAAWAEMTCFQVLGWQFPLHIFDLHTAYLAASNALL
                     PRSPDQVRRKPRKRLPDACRAYGIEGWERIDKHTTAEAIGQGRWREYGREAVLDYCEE
                     DVRMSVRLLRAQLRRHQCGPTILPAADVPRVLHWSNYSAKAVAQIQSRGMPIDVPLWN
                     LVQENKQAVIGKLLRGFDPSYGSEEPIYTTDGEWSYARFERFLVRSGVTAWPRLESGK
                     LDIDGDAFRMMSHIPGIEGLHALRDTLGVIARAKLPIGHDGRNRPSLFPFCTATGRNA
                     HAKSLFNAHASVRSFMAFSPDAIGVYLDWRTQEVGIAAALSEDRALIEAYRDGDVYHS
                     LALVCGLTDDTDLKRWKRECPDVRQRMKALQLGINYGMGVPSLARGLDRHPIIASNFI
                     ERHRRTYPRFWDWRDNEVQVAMLERRIE"
     gene            complement(1431..>2099)
                     /locus_tag="VM910_13035"
     CDS             complement(1431..>2099)
                     /locus_tag="VM910_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63493.1"
                     /translation="QRKPQMSKPELVPTDKPTTEATDSIAKPEGFDLDRFKSKRAAAM
                     ANVETLPNALPHHSIAQAKDFVRLHPDEIKYWSSELCFVSVPIKGSKRDTLHLIDEDL
                     AMRFLPSAKIQRFRLALASKPNDVFFLCQVPSRNLDNTYNSSNLEACEQAKTFWTQAT
                     SRREEGVEAYKITFARHRDTFPEPKWLPQSLAELIEKTFAGRMIDNENHPGLLRLVGA
                     RQSS"
BASE COUNT          383 a          635 c          637 g          444 t
ORIGIN      
        1 ctcgattcgg cgctccagca tcgccacttg cacttcgttg tcacgccagt cccagaatcg
       61 cggatacgtt cgccgatgtc gttcgatgaa gttgcttgcg atgataggat gccggtcgag
      121 gccgcgcgcc aaggaaggga cacccatccc ataattgatc ccgagctgca gcgctttcat
      181 gcgctgccga acgtccgggc actcccgttt ccagcgcttc aagtccgtgt cgtcagtgag
      241 gccgcaaacc agagcgagcg agtggtagac gtcaccatcg cggtacgctt cgatcaatgc
      301 acggtcttcg gagagagctg cggcgattcc aacctcctgc gtcctccaat cgaggtagac
      361 gccgatcgcg tcgggcgaga acgccatgaa cgagcgcacg ctcgcgtgcg cattgaacag
      421 gctcttagcg tgcgcattgc ggccggtggc cgtgcagaac gggaacaagc tcgggcggtt
      481 gcgaccgtca tgcccgatcg ggagcttcgc tcgcgcgatg acgccgagcg tgtcgcgcag
      541 cgcatgcagg ccctcgatcc cagggatgtg gctcatcatt cggaaagcat cgccatcaat
      601 gtcgagcttg ccgctctcca atcgcggcca cgctgtcacg cctgagcgga cgagaaaccg
      661 ttcgaatcgg gcgtagctcc actcgccgtc ggtcgtgtag atcggctcct cgctgccgta
      721 gctcgggtcg aaaccacgta gtagtttgcc aataacagcc tgtttattct cctgcacgag
      781 gttccacagc ggcacgtcga tcggcatgcc gcggctctgg atctgtgcaa ccgccttggc
      841 gctgtaattc gaccagtgca gcacgcgcgg cacgtcggcc gctggcaata tcgttggccc
      901 gcattggtgg cgtcgcagct gcgcacgcag cagccgcacc gacatccgga cgtcttcctc
      961 gcagtagtcg aggacggctt cgcggccgta ctcgcgccag cgtccctgtc cgatggcttc
     1021 tgcagtcgtg tgcttatcga ttcgctccca gccctcgatg ccgtaggcgc ggcacgcgtc
     1081 gggcagtcgt ttgcgcggtt tcctccgcac ttggtccggg ctgcgcggca ggagagcgtt
     1141 gctggccgcg aggtaagccg tgtgaaggtc gaagatgtgg agtgggaatt gccatcccag
     1201 cacttggaag caagtcatct ccgcccaggc ggcgtaggca acaaagaggg cgtcgggacc
     1261 agtatcgaaa ggcgggaccg agccgaagtc gccgcgccag agtcgcacgg tgtgcacgtg
     1321 ctcgagctgc tcgtccaaca cgtacgccac catgcacagc acgttgggca tgctgccgga
     1381 tgctacttca tactcaaagt cgcacacgac aatcgtgccg aagttcttgc tcacgacgat
     1441 tgccgtgctc caaccaagcg caggaggccg ggatgatttt cgttgtcgat catgcgacca
     1501 gcgaaagttt tctcgatgag ctccgcgagc gactgcggca accacttagg ctccgggaac
     1561 gtgtcgcgat gacgtgcgaa ggttattttg tatgcttcaa caccttcctc gcgcctgctc
     1621 gtcgcctgcg tccagaatgt cttggcctgt tcgcaagctt cgagattgct gctgttgtag
     1681 gtgttgtcca agttccggct tggtacttgg catagaaaga acacgtcatt gggcttcgac
     1741 gcgagcgcca gtcgaaagcg ttggatttta gcgctcggca agaaccgcat tgcgaggtcc
     1801 tcatcgatga gatgcagtgt atcccgcttc gatcccttga tggggacgct cacgaaacaa
     1861 agctccgacg accaatattt aatttcgtct gggtgcaacc ggacgaagtc ctttgcttgc
     1921 gcgatcgagt gatggggcaa cgcgttcggc aatgtctcga cgttcgccat cgccgcggcg
     1981 cgcttggatt tgaacctatc gaggtcaaac ccctccggct tggcgatcga atccgtcgct
     2041 tcggtagtgg gcttgtcagt cggcactagt tcaggtttgc tcatctgggg cttcctttg
//
LOCUS       DATLJT010000566         9765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4169757,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000566 DATLJT010000000
VERSION     DATLJT010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4169757"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VM910_13040"
     CDS             <1..335
                     /locus_tag="VM910_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI63494.1"
                     /translation="RQITLNLIGNSIHLANPAGQVIVSTALSDFGEVVLRVRDTGHGL
                     DDNVVAAAMEPFRTRPPSDETSNTSPVSLSLTKALVEANRAKFQIKNDRRSGTLIEVV
                     FPRAVARA"
     gene            complement(344..1546)
                     /locus_tag="VM910_13045"
     CDS             complement(344..1546)
                     /locus_tag="VM910_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HVI63495.1"
                     /translation="MAVPRYRDYQGWPVLAAGFRPFFLAGAIQAAVAILVWLPVFFGE
                     VSLTSAFAPRDWHVHEMLYGYLPAVITGFLFTAIPNWTGRLPIQGTPLLGLVIVWIAG
                     RVCVTFSAETTWWVAMLVDAGFLLMVALAAAREIVAGRNWRNLPVVLLVVFLLAGNVT
                     FHLEAHFRGAADVAIRIGIGVVVLLISLIGGRIIPSFTRNWLVRENPGRLPAPFGRFD
                     MLVVALGGLALVCWIVAPDYRATGVALALAGLLHFARLARWAGERTLRERLLLILHVG
                     YAFVPLGFLLNALAAFGYVLPSAGVHAWMVGGAGIMTLAVMTRATLGHTGQELTASAA
                     TQAIYAAIVVAALARICAVLDPAHSEILLHVAAFAWVVAFFGYAIAFGPLLLGIRGRA
                     LGVRKLAA"
     gene            1685..2407
                     /locus_tag="VM910_13050"
     CDS             1685..2407
                     /locus_tag="VM910_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI63496.1"
                     /translation="MTTVDSSLVAHLPLFAGITADDLAEILREARSTRYAKNSAVFEQ
                     GEDAQSFFLLLHGHVRASKTTPTGEQIVVRYVAPGEIFGVAPAIGIARYPATATAVDD
                     SVVLSWPSAAWPRLVARFPQLATNTLQTVGSRLQETHTRVIEMSTQQVEQRIAHALLR
                     LAKQSGRKLDHGVEIDFPISRQDIAQMTGTTLHTVSRILSGWEQLGLIESGRQRIVLR
                     EPHRIFVLAEHGPNDGEPPRRA"
     gene            complement(2417..2608)
                     /locus_tag="VM910_13055"
     CDS             complement(2417..2608)
                     /locus_tag="VM910_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HVI63497.1"
                     /translation="MPISSYDLVDEVMRARPETIHVFLRYRMGCVGCPIATFHTVEDA
                     CREHGVDRDVFMEALCACT"
     gene            complement(2689..4038)
                     /gene="hemN"
                     /locus_tag="VM910_13060"
     CDS             complement(2689..4038)
                     /gene="hemN"
                     /locus_tag="VM910_13060"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HVI63498.1"
                     /translation="MRSDLAAGYGQERLPRYTSYPTAPHFSEAIGAENYAEWLKAIPR
                     NSTASLYLHVPFCRSMCWYCGCHTSVARRDEPIAIYESALRSEIDLVSRLIDRRIKVD
                     HVHFGGGTPTIMTPESFTDLIGSIRQTFFVLPSAETAIEIDPRTLAVRMIDALALGGV
                     NRASLGVQSFDPVVQRAINRVQSFEETAAATENLRRAGISAINFDLIYGLPHQTIASC
                     LDTVRRCISLRPDRFAVFGYAHVPTFKKYQRRIDESRLPDSLERFEQSCAIANALTQA
                     GYVQIGLDHFALPGDAMALAQREGRLRRNFQGYTTDRSSVLLGFGASAIGHLPQGFVQ
                     NEIGIGAYSQLIAAGELATAKGYALTADDRFRGEIIERIMCDFGVDLGPICVRHGLVP
                     DAIVGSPRLKELVSDGVVTLDGSLLSVADNSRFLVRSVAAAFDAHLDGSGRLHSRAV"
     gene            4324..5415
                     /gene="nirK"
                     /locus_tag="VM910_13065"
     CDS             4324..5415
                     /gene="nirK"
                     /locus_tag="VM910_13065"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HVI63499.1"
                     /translation="MLTRRAAMFSAAAAALLLATSSFAAEPNLPRQKVELVAPPFVHP
                     HEQATKQGPKIMEFRLVVEEKKVVIDEKGTTFQAMTFNGSMPGPLMVVHEGDYVEVTL
                     VNPATNSMPHNIDFHSATGALGGAALTLINPGEQVVLRWKATRTGVFVYHCAPGGPMI
                     PWHVVSGMNGAVMVLPRDGLNDGHGHPLKYDRIYYVGEQDLYVPRDEKGNFKSYDSPG
                     EAYTDTEEVMRKLIPTHVVFNGKVGALTGKNALKANVGENVMIVHSQANRDSRPHLIG
                     GHGDYVWETGKFSNAPETGLETWFIRGGSAGAAMYKFLQPGIYAYVTHNLIEAADLGA
                     TAHFKVEGKWNDDLMTQVKAPAEIPAVTN"
     gene            5475..6347
                     /locus_tag="VM910_13070"
     CDS             5475..6347
                     /locus_tag="VM910_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HVI63500.1"
                     /translation="MLIAFKLKLLLACAAGLAGPLAVAPIMSYLSARDRILPEQAIVE
                     LKSGHFAYRVAGDFTRAGKPAEAPLVQLSFAGPLTIMKRQVSSAEYQACVDDGGCRAL
                     DRDVVVAIDRPAVQVSWHDAEAYASWLSHRTGVTYRLPTDEEWAFAAGSKFADDGVPV
                     DDNNPAKRWIARYERETGRDAGTATRAFGGYGTNENGLSDLSGNVWEWTTTCFVRSTL
                     DESGRVVGQSKNCGVRIAEGAHRAYVTDFIRDARAGGCAAGTPPDNLGFRLVREPPSW
                     TSMRLGKALSFTTS"
     gene            6482..7318
                     /locus_tag="VM910_13075"
     CDS             6482..7318
                     /locus_tag="VM910_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HVI63501.1"
                     /translation="MRCLIVADLHYSLPQFDWLLSAAPQFDLVIFAGDALDIGSAVDF
                     RAQILVVKKYLSRLSGLTHVALCSGNHDLDERSADGEKVARWIDEVRQLGIACDGDSL
                     TIGDTLFTICPWWDGPVVRDRIDAQLREAAALAAATAKRWVWVHHAPPANSPTSWGGK
                     RFFGDVELVQWIERYQPSIVISGHVHQSPFIPDGSWFDRLGATWVFNTGLQPGRPPTH
                     IVLDLAQGTAFWLAAAEQQWIDLNAPIKRPAAPIEEPPAWLISLGRIADPILARPAGV
                     EG"
     gene            complement(7257..7742)
                     /locus_tag="VM910_13080"
     CDS             complement(7257..7742)
                     /locus_tag="VM910_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI63502.1"
                     /translation="MREILDYCTGGNRREVPAGSLVLREGTKTGRLFVLVEGRLEVIR
                     GNSVVAVLAEPGALTGEMSLLLDRPHTATVRAAVDSTIYEINDASSFLDEHPAAARLI
                     AQLLAQRLNVATTYLADLMKQYAGHGTHLEMIGELLQSMISLPPQQVSPGSDRQSDPR
                     I"
     gene            complement(7865..8659)
                     /locus_tag="VM910_13085"
     CDS             complement(7865..8659)
                     /locus_tag="VM910_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI63503.1"
                     /translation="MDRRLAGGTVNAAETGRGTPLFLFHSLLSDRASFDTIVPQLSQS
                     FRVIVPELPGFGGSQAVAGSLADIADRMTEAVHDGARGEGAILLGNGYGGFVALQMAI
                     RHPGIATRLIFADCGAAFPEAGREAFRNMAAASRAKGLAAIVDVAMRRLFAPEFQAEH
                     PELMDDRRAAFLRTDPQVFRAACEALAGLDLRGDLQKVTVPTLVLVGEHDEATPPPMS
                     RELAAGLPDARLKIISGCAHVPQLQAPKTFLEAVSDFLAAKSSAPA"
     gene            8797..9447
                     /locus_tag="VM910_13090"
     CDS             8797..9447
                     /locus_tag="VM910_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI63504.1"
                     /translation="MLLRDNVYESLRADILACRLAPGDDMREQELAERYAVSRQPVRE
                     ALLRLAREHLVTVQPRQGYRVNPISLADARDILRFRLALEPACVAEAIENASDATLKG
                     LDRFRRFSGDHEKFIAYNRAFHSALAAASNNRRMAAALCDLIGQADRLVRVSVANLRG
                     HDPKKLVAEHVALIEAMQRRDGRSAARIIKAHITATEKRVLPALRRNAVIVEGRDA"
     gene            9444..>9765
                     /locus_tag="VM910_13095"
     CDS             9444..>9765
                     /locus_tag="VM910_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI63505.1"
                     /translation="MNISAKTVEDEVHGNFVDGREIEAGSGQMLDVRNPATGEVIAKI
                     PNSTADDVDCAMKSARAAFDGKAWGGMDVRSRARLVNRLADAFEANLEQLYRLETLNN
                     GRPVN"
BASE COUNT         1961 a         3153 c         3015 g         1636 t
ORIGIN      
        1 tgcgccagat cacgctgaac ctgatcggca actcgatcca cctcgccaat cccgcggggc
       61 aagtgatcgt ctcgacggcg ctgtcggatt ttggcgaggt ggtgctcagg gtacgcgaca
      121 ccggccacgg gctggacgac aatgtggtcg cggccgcgat ggagccgttc cgcacgagac
      181 cgccctcgga tgagacttcc aacacttcgc ccgtcagcct gtcgctgacc aaggcccttg
      241 tcgaagccaa tcgcgcaaaa ttccaaatca aaaacgaccg gcgaagcggg accttgatcg
      301 aagtggtgtt cccacgcgcc gtggcgcggg cctgagcttc aactcaggcg gctagtttgc
      361 gcacgccgag cgcccggccc ctgataccca aaagcagcgg cccaaacgcg attgcatagc
      421 cgaaaaacgc aacgacccag gcgaaggccg cgacatgcag caggatttcg ctatgcgcgg
      481 gatcgagcac ggcgcaaatg cgggcgagcg ccgcaacgac aatggcagcg taaatcgcct
      541 gcgtcgccgc cgaggccgtc agctcttggc cggtgtggcc gagagttgca cgggtcatca
      601 cggccagcgt catgatgccc gcgccgccca ccatccaggc atggaccccg gcgctcggca
      661 ggacatatcc gaacgctgcc agcgcgttga gcaggaatcc gagcggcacg aatgcgtagc
      721 cgacatgcag gatcaacaag agccgttcac gcagcgtccg ctcgccggcc cagcgcgcga
      781 gtcgcgcgaa atgcaacagg ccggccagcg caagggctac gccggtcgcg cgatagtctg
      841 gcgcaacgat ccagcacaca agggccagcc cacccagagc cacgacgagc atgtcgaaac
      901 ggccgaatgg cgcaggcagc cggccgggat tttctctcac cagccagttc cgggtaaaac
      961 tcggaatgat gcggccgccg atcagcgaga tcaaaagcac cacaacgccg atcccgatac
     1021 gaatcgcgac atcggcggcg ccgcgaaaat gcgcctcgag atggaaggtg acattgccgg
     1081 cgagcagaaa cacgaccagc agcaccaccg gcagattgcg ccaatttcgg cccgcgacaa
     1141 tctcgcgggc ggcagcaagc gcaaccatca gaagaaagcc ggcatccacc agcatcgcaa
     1201 cccaccaggt cgtctccgcg gaaaatgtca cgcacaccct gcctgcgatc caaacgatca
     1261 ccaggccaag cagcggcgtt ccctggatcg gcagccgccc ggtccagttc ggaatcgcgg
     1321 tgaacagaaa accggtgatc actgccggca gatagccgta gagcatttca tgcacatgcc
     1381 agtcgcgcgg cgcgaacgcc gaggttaacg acacctcgcc aaaaaacacc ggcagccaga
     1441 ccaggattgc gacggcagcc tggatcgcgc cggccagaaa aaacgggcgg aagcccgccg
     1501 ccagcaccgg ccagccctga taatcgcgat agcgcggaac tgccattgtc caaacctcga
     1561 tgttgatcca gattatttac gccgaaccat ggcagctcaa attgagccag cgcaaacttc
     1621 cgctaacatg gaaccgcatt cgcaggtccg gatcgcaaaa aggcgctgcg ccacggagac
     1681 aatgatgacc acagtcgaca gctcgctggt cgcccatttg ccgctgtttg ccgggattac
     1741 ggcggatgat ctcgccgaga tcctccgcga agcccgctcc acacgctatg cgaaaaacag
     1801 tgccgtattc gaacagggcg aggacgcgca atccttcttt cttctgctgc acggccacgt
     1861 gcgcgccagc aagaccacgc cgaccggcga gcagatcgtg gtgcgctatg tcgcgcccgg
     1921 cgagattttc ggcgtcgcgc ctgcgatcgg gatcgcgcgt tatccggcga ccgcgaccgc
     1981 agtcgacgac agcgtggtgc tgagctggcc ttcggcagcc tggccgcggc tggtggcacg
     2041 ctttccgcag cttgcgacca acacgctgca gacggtcggc agccgacttc aggaaacgca
     2101 tacgcgcgtc atcgagatgt cgacgcagca ggtcgagcag cggattgccc atgcgttgct
     2161 gcggctcgcc aagcaatccg gacgcaagct cgatcacggc gtggagatcg atttcccgat
     2221 cagccgtcag gacatcgcgc agatgaccgg caccactttg cacaccgtaa gccgcatcct
     2281 gtcgggttgg gagcaactgg gcctgatcga aagcggcagg cagcgcatcg tgctgcgcga
     2341 gccgcaccgg attttcgtgc tggccgaaca cggcccgaac gacggcgagc cgccccggcg
     2401 cgcttgacgg cactcgtcat gtacaggcgc agagcgcctc catgaacaca tcgcgatcga
     2461 cgccatgctc gcggcaggca tcctccaccg tgtggaaggt cgcgattggg cagccgacgc
     2521 aacccatcct gtatctgagg aagacgtgga tcgtctcggg cctggcccgc atcacttcgt
     2581 cgaccagatc gtaagacgaa attggcatgc gcatctccgt tcggacgaga acgatagcgc
     2641 gcctcttcga aaagttgttt gtcgcggagc aaactcgcaa agatgttcct acacggcccg
     2701 gctgtgcagc cgcccggaac cgtcgagatg tgcgtcgaat gcagccgcaa cgctgcgcac
     2761 caggaaccgc gaattgtcgg caaccgacaa caggctgccg tccagcgtga cgacgccgtc
     2821 ggacaccagc tccttcaggc ggggcgagcc aacgatggcg tccggcacca atccatgccg
     2881 gacgcaaatc ggaccgaggt ccacgccgaa gtcgcacata atgcgctcga tgatctcgcc
     2941 acgaaaccgg tcgtcggccg tcagcgcata tccctttgcg gtcgcaagct cgccggccgc
     3001 gatcaattgc gaataggcgc cgatcccgat ttcgttctgc acaaagccct gcggcagatg
     3061 gccgatggcg ctggcgccga acccaagcag gacgctgctt cggtcggtgg tgtagccctg
     3121 gaaatttcgc cgcagcctgc cttcgcgctg cgcaagcgcc atggcatcgc cgggaagcgc
     3181 gaagtggtcg agcccgatct gcacgtaacc cgcttgcgtc agcgcattgg cgatcgcgca
     3241 cgactgttcg aagcgctcga ggctgtccgg cagccgggat tcgtcgatcc tgcgttgata
     3301 tttcttgaag gtcggcacgt gcgcgtagcc gaacacggcg aagcgatcgg ggcgaaggct
     3361 gatgcagcgc ctgaccgtgt cgaggcatga cgcaatggtc tgatgcggca atccatagat
     3421 cagatcgaag ttgatcgcgg atatgccggc gcggcgcagg ttttcggtcg cggccgccgt
     3481 ctcctcgaaa ctctgcacgc ggttgatggc gcgctgaacc acggggtcga agctctgcac
     3541 cccaaggctc gcgcggttga cgccgccgag cgccagtgcg tcgatcatcc ggaccgccag
     3601 cgtgcgcggg tcgatctcga ttgcggtctc ggcggaaggc aacacaaaga acgtttgacg
     3661 gatcgatccg atcaggtccg tgaacgactc cggcgtcatg atcgtcggcg tgccgccgcc
     3721 gaaatgaacg tggtcaacct tgatgcggcg atcgatcagc cgcgacacca gatcgatctc
     3781 actgcgcagc gccgactcgt aaatggcgat cggttcgtcg cggcgggcga ccgaggtgtg
     3841 gcagccgcaa taccagcaca tggagcgaca gaacggcaca tgcagataga gcgaggcggt
     3901 ggaatttcgc gggatcgcct tcagccattc ggcatagttc tccgcgccga tcgcttccga
     3961 aaaatgcgga gcggtgggat agctcgtata tctcggcaat cgctcttgcc catagcccgc
     4021 cgccagatct gacctcattt ccgaccattc cctgctcgaa ggcgcaaaag agcacccacc
     4081 atccattaac aatagctgca tgccgaacat cgactttgcg ctggctcaaa ggcgcgacgc
     4141 gaactccggc cgcggacatg gtcagggtcg agccatgaat cctgctgcgc cggcctccgt
     4201 agaaatcccg acgtcggaga aaccccaaag gagcggaatg ttgttgcagc gcaaacaggc
     4261 tttacgtcaa tcggcgcaaa gtgctcgcgc taagtcaaaa catccagatg aaggaatatt
     4321 ccgatgttga cccgcagagc cgcaatgttc agcgcagccg ccgcagccct cctgctggcg
     4381 acgtcttcgt ttgccgcaga acccaatttg ccgcgccaga aggtcgagtt ggtcgcgccg
     4441 cctttcgtgc accctcacga acaggccacc aagcaaggcc ccaagatcat ggaattcagg
     4501 ctcgtggtcg aggagaagaa ggtggtcatc gacgagaagg gcacgacctt ccaggccatg
     4561 actttcaacg gctcgatgcc gggaccgctg atggtggtgc atgagggcga ctatgtcgaa
     4621 gtgacgctgg tgaaccccgc caccaactcg atgccgcaca atatcgactt ccactccgca
     4681 accggcgcac tcggtggcgc cgcgctcacg ctgatcaatc ccggcgagca ggtcgtgctg
     4741 cgttggaagg ccacccgcac cggcgtgttc gtctatcact gcgccccggg cggtccgatg
     4801 atcccctggc atgtcgtgag cggcatgaac ggcgcggtca tggtgctgcc gcgcgatggt
     4861 ctcaacgacg gtcacggcca tccgttgaag tacgaccgca tctattatgt cggcgaacag
     4921 gatctctacg tgccgcgcga cgagaagggc aacttcaaat cctacgactc gcccggcgaa
     4981 gcctacaccg acacggaaga agtgatgcgc aagctgatcc cgacccatgt cgtgttcaac
     5041 ggcaaggtcg gcgcgctgac cggcaagaac gcgctgaagg ccaatgtcgg cgagaacgtg
     5101 atgatcgtgc attcgcaggc caatcgcgac agccgcccgc acctgatcgg cggccatggc
     5161 gactatgtct gggagacggg caagttctcg aacgcgccgg agaccgggct cgagacgtgg
     5221 ttcattcgcg gcggctctgc gggagcggcg atgtacaagt tcctgcagcc cggcatttac
     5281 gcctacgtca cgcataatct gatcgaggcg gccgatctcg gcgccaccgc gcacttcaag
     5341 gtcgagggca agtggaacga tgacctgatg acccaggtga aggcgcctgc ggaaatcccg
     5401 gccgtcacca actgacgacg aaaaaggggc cgacgaagtg ccggcccctt ccctcctgga
     5461 aatgcggggc agccatgctg attgccttca aactcaagct cctgctcgct tgcgctgccg
     5521 gacttgccgg cccgctggcg gtcgcgccga tcatgtccta tctctccgcg cgcgatcgca
     5581 tccttcccga acaggcgatc gtggaattga aatccggcca tttcgcttac cgcgtagccg
     5641 gcgacttcac ccgtgcgggc aagcccgctg aagcgccgct tgtgcaactg tcctttgccg
     5701 ggccgctcac aatcatgaag cgccaggtct cgtccgccga gtatcaggcc tgcgtcgacg
     5761 acggcggctg ccgcgcgctc gaccgcgatg tggtcgtcgc gatcgatcgg ccggccgtgc
     5821 aggtgagctg gcacgatgcg gaagcctatg cgagctggct gtcgcacagg accggcgtaa
     5881 cctatcgtct gccaacagac gaagaatggg cgttcgcggc cggcagcaag ttcgcagatg
     5941 atggcgtgcc ggtcgacgac aacaatcctg caaaacgctg gattgcacgc tacgagcgcg
     6001 aaaccggccg cgacgccgga accgcaaccc gcgccttcgg cggatacggc accaatgaaa
     6061 acggcctctc cgatctctca ggcaatgtct gggagtggac cacgacctgc ttcgtgcgca
     6121 gcacgctcga cgaatccggc agggtcgtcg gtcagagcaa gaattgcggg gtccgtatcg
     6181 ccgagggcgc gcaccgcgcc tacgtcacgg acttcatccg cgacgcgcgc gccggcggct
     6241 gcgctgcggg cactccgcct gacaatctcg gctttcggct tgtccgcgaa ccaccgtcat
     6301 ggacgagcat gcgcctcggc aaggcactct cgttcacgac ctcttgaacc gccacctttc
     6361 aactgcgcat ggtgtcgcag acctgcgcgc atgtgtcgct tccggcggtg ctgcgccggt
     6421 acggattttg ctctgagctg agcggcttac tcatctcgaa gcgtccggaa tttctagact
     6481 tatgcgttgt ctgatcgttg cggatttaca ctattcgttg ccgcagtttg actggctgct
     6541 cagcgcggcg ccccagttcg atcttgtcat cttcgccgga gatgcgctcg atatcggctc
     6601 ggcggtcgat ttccgtgccc agattttggt cgtgaagaaa tacctctcgc ggctgtcggg
     6661 cctgacgcat gtcgctctct gttccggcaa ccatgatctg gatgagcgca gtgcggacgg
     6721 cgaaaaggtc gcgcgctgga tcgacgaggt cagacagctt gggatcgcct gcgacggcga
     6781 cagcctgacc atcggcgaca cgctgtttac gatatgccca tggtgggacg ggccggtagt
     6841 cagggaccgc atcgacgcgc aacttcgcga ggcggcagcg ttagcggcgg ccacggcgaa
     6901 gcgatgggtc tgggttcatc acgcgccgcc ggcgaactct ccgacaagct ggggcggcaa
     6961 gcgctttttt ggcgacgtcg agctcgtaca atggatcgag cgttaccagc cgtcgatcgt
     7021 gatctcgggc catgtgcatc aatcaccctt cattcccgac ggatcatggt tcgatcgact
     7081 tggcgccacc tgggtcttca ataccggcct gcagcctggc cggccgccga cgcatatcgt
     7141 gctcgatctg gcccaaggca cggcattctg gcttgccgcc gccgagcaac aatggatcga
     7201 cctcaacgcg ccgatcaagc ggccggcagc gccgatcgaa gagccgccgg cctggcttat
     7261 atccttgggt cggattgccg atccgatcct ggcgagacct gctggggtgg aaggctgatc
     7321 atgctttgca gaagttcgcc gatcatctcg agatgggtac cgtgaccggc gtattgcttc
     7381 atgagatcgg caaggtaggt tgtagccacg ttcagccgtt gcgccagtaa ttgtgcgatc
     7441 aatcgcgccg cggccggatg ctcgtcgagg aaggacgacg catcattgat ctcatagatc
     7501 gtggaatcaa cggctgcccg taccgtcgcg gtgtgaggcc ggtcgagcaa cagcgacatc
     7561 tcgccggtca gcgcaccggg ttcggcgagc actgcgacaa cactgttgcc cctgatgact
     7621 tcgagccgcc cctcgacgag gacaaacagc cgcccggttt tggtcccctc gcgaagcacg
     7681 agggagccgg caggcacctc tcgccggttt ccacccgtgc aatagtccag aatctcgcgc
     7741 atcggccaac acccttgttc gaggttaacc ccgacggata acagcgtcga agcgataatg
     7801 taggcccggt atgcccaaat tctcggcaac ctcaggcacc ggccgctgag cggcgttgcc
     7861 tcgattatgc cggtgcggaa ctcttggccg caaggaagtc cgagacggct tcaaggaatg
     7921 tcttgggcgc ctgcagttgc gggacatgcg cgcaacccga aatgattttg agccgcgcat
     7981 ccggcagacc tgccgccagt tcacgcgaca tcggcggagg cgtcgcttca tcgtgctcgc
     8041 cgaccaagac aagtgtcgga accgtcacct tttgcaaatc tccgcgcagg tcgagcccgg
     8101 cgagcgcttc acaggctgcg cggaacacct gcggatcggt ccgcagaaac gccgcgcggc
     8161 gatcgtccat caactcggga tgttcggcct gaaactccgg tgcgaacagc cgacgcatgg
     8221 caacatccac gatcgccgcg agccccttcg cccgcgacgc cgcagccatg ttgcgaaagg
     8281 cctcgcgccc tgcttccggg aaggctgcgc cgcaatcggc gaagatcaat cgcgtcgcaa
     8341 tgccgggatg acggattgcc atctgcagcg cgacgaagcc gccatagcca ttgccgagca
     8401 agatcgcgcc ttcgccgcgc gctccgtcat gcaccgcctc cgtcatgcgg tcggcgatat
     8461 cggcaaggct gccggcgacc gcctgcgagc cgccgaagcc cggcagttcc ggaacgatga
     8521 cgcgaaacga ttgcgacagt tgcggcacga tcgtgtcgaa gctcgcgcga tccgagagca
     8581 gggagtgaaa caaaaacagc ggcgtgcccc gccccgtttc tgccgcgttc acggtcccgc
     8641 cagccaaacg ccgatccatg tcctgccctc gccctcccgc acaagtcgcg ggacgatccg
     8701 cgattgatag atcaaaccgg gggcgtttca agcattaact ggcttgacgt gatatttcac
     8761 gattgcgaca ctgccggctc gcaggaagga aaatcaatgc ttttgcgcga taacgtctac
     8821 gaaagcctgc gtgctgatat tcttgcctgt cgcctcgcac cgggtgatga tatgcgcgaa
     8881 caggagctcg ccgaacgcta tgcggtgagc cgccagccgg tacgcgaggc gctgctcagg
     8941 cttgcgcgcg agcatctggt gaccgtgcag ccgcgccagg gctaccgcgt caacccgatc
     9001 tcgctggcgg atgcgcgcga cattctgagg tttcgtctcg cgctggagcc ggcctgcgtt
     9061 gcggaagcga tcgagaacgc gagcgatgcg acgctcaagg gcctcgaccg gttccggcgc
     9121 ttctccggcg accacgagaa attcatcgcc tataaccgcg ctttccattc ggcgctcgcg
     9181 gcagcctcga acaaccgccg catggccgca gcactgtgcg acctcatcgg gcaggccgac
     9241 cgcctggtgc gggtgagcgt cgccaacctc aggggccacg atcccaagaa gctggtcgcc
     9301 gagcatgtcg cgctcatcga agcgatgcag cgccgcgacg gccgcagcgc cgcgcggatc
     9361 atcaaggcgc atatcaccgc gaccgagaag cgcgtgctgc cggcgctgag gcgcaacgcc
     9421 gtgatcgtcg aagggaggga cgcgtgaaca tttcagcaaa aaccgtcgag gacgaggtgc
     9481 acggcaattt cgtggacggg cgcgaaatcg aagcgggctc aggccagatg ctcgacgtgc
     9541 gcaaccccgc gaccggcgag gtgatcgcga agattccaaa ttcgactgcg gacgacgtcg
     9601 attgcgccat gaagagtgcg cgcgcagcct tcgacggcaa ggcctggggc ggcatggacg
     9661 tccgatcgcg ggccaggctc gtcaatcggc tggcggatgc gttcgaggcc aatctcgagc
     9721 agctctatcg cctcgaaacg ctcaacaatg gacggccggt caacg
//
LOCUS       DATLJT010000567         6159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3709841,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000567 DATLJT010000000
VERSION     DATLJT010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709841"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..933)
                     /locus_tag="VM910_13100"
     CDS             complement(34..933)
                     /locus_tag="VM910_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI63506.1"
                     /translation="MSKRATGTLADPTPLRVRLALRDLDGVTLLALPAVLFVVVLFIY
                     PFAYGFVLSFQPKAGNWLANYTKFFSDPFLYDTIGATFRLAIPATLINVLISVPVALR
                     VRRMDYRRLLTAILVIPITLGTVLIAEGLLIYLGPQGWFNRVLFMIGLGKIRLVHNYT
                     GVMLSLILSGFPFTFLLTLSYLTGIDPSLEQAASTLGAKARARFVHVLLPLLAQGLAI
                     NLCLSFVQTFSVFPSAVLLGAPAGDTRVISIAAYQAAYEQYDSSMASAIAMIMGFCQL
                     IVVVMILFGRSLAYRGAASGGKG"
     gene            complement(930..1994)
                     /locus_tag="VM910_13105"
     CDS             complement(930..1994)
                     /locus_tag="VM910_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63507.1"
                     /translation="MAIAARDFRELRLESMSRTFGSINALKGVTLSVRRGEFIALLGP
                     SGCGKSTALNCLAGLLTLSGGSVWLDTTRIDHLPPEARGFGMVFQNYALFPHMSVRRN
                     IGFGLNMHGIAKDKIERKVDEALSIVRLGDQGDKLPGQLSGGQQQRVAIARAIVIEPP
                     LVLMDEPLSNLDAKLRLEMRAEIRRIHELIGSATIYVTHDQEEALSLADRIVVMRAGE
                     VRQVGTPEELYLRPAHADVAEFMGYRNQLRVRATRADKLIHVNVAGANLPATPVDAVN
                     EGEVVAAIRPDDLAVSATGSIAATVESAQYHGHSFYCSGRIADGTELYFQSDRRVSKG
                     ETVRLTADPGRVLVYSRATA"
     gene            complement(2084..3256)
                     /locus_tag="VM910_13110"
     CDS             complement(2084..3256)
                     /locus_tag="VM910_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI63508.1"
                     /translation="MKLTRRAVVGGLASSAAAPAFAQNLPLPTSPVTLNFVDVAGNLA
                     LTQKAIEAYRDNNPKLVAKVNFSKAPAPELPGKIKAQQDAGRVDIDGVLTGIDALSAG
                     VDQKLWIPIATEFAAKLPKLNEIYLPGAARMQELASNQGVCIVFCPAGPVMEYMPDNV
                     KQVPQTAEDLLAWAKANPNRFMYARPANSGPGRVFMMGLPYILGDKDPHDPKDGWDKT
                     WSYLKELGQYVEYYPAGTGATMKELGEGSRDMIPSQMGWDINPRALGVVPKEAKVFFL
                     KGYHWVLDAHYLCIPKGVSNDKLAVLIDFLNYMLSPPAQAMTYDDGYFYPGPAVKDVP
                     LSMAPEASQKVIAEFGRSEYAQAITAAPQEIPLLPDKLVYAFSRWDEQIGSQKKKN"
     gene            complement(3353..4066)
                     /locus_tag="VM910_13115"
     CDS             complement(3353..4066)
                     /locus_tag="VM910_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HVI63509.1"
                     /translation="MLSVALRDKLKTVSTATICTALFKRGFRNQYIQDVTPLNPAAGT
                     MVGEAFTLRYIPAREDLNLITVFQDRSHPQRKAVEECPPGAVFVIDSRKDARAASAGS
                     ILVTRLMIRGVAGVVTDGGFRDSPEIARMAIPAYHHRPAAPTNLTVHQALDINVPIAC
                     GDAPVWPGDVVVGDAEGVVVIPAHLADEIANEAVEMTAFEDFVTEEVKNGRSIIGLYP
                     PTDEQAKEDFSVWRKLKGR"
     gene            complement(4078..5811)
                     /gene="araD"
                     /locus_tag="VM910_13120"
     CDS             complement(4078..5811)
                     /gene="araD"
                     /locus_tag="VM910_13120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HVI63510.1"
                     /translation="MSRKQPKELRSARWFLPDDFRSFGHRSRMSQMGYSGADYVGRPV
                     IGIINTWSDLNQCHAHFKQRVDDIKRGVFQAGGFPVELPALSLSESKVKPTTMLYRNM
                     LAMETEELIRSHPVDGAVLMGGCDKTTPGLLLGATSSGVPAIYVPAGPMLRGNWHGKV
                     LGSGSDAFKYWDERRAGALSEKAWSEMEVGIARSYGVCMTMGTAATMTALAEAIGMAL
                     PGASSIPAPDSGHIRMCADSGRRIVEMVWEDVTPAKIQTEAAYRNAIVVAMAMGCSTN
                     AVIHLLAMARRAGHSINLDDFDRASREVPVIANIRPSGDKYLMEDFYYAGGLPALMSR
                     LKPFLDLSHVNVSGSRLGDVLDSAEIYNDDVIRPLNNPIYAEGALAVLRGNLAPNGCV
                     MKPSAAAPHLLRHSGPAMVFDSYPDLKANIDRDDLDVTSDHVLVLRNAGPLGGPGMPE
                     WGMLPIPKTLLKQGVRDMVRISDARMSGTSYGACILHVSPESYVGGPLGLVKTGDMIT
                     VDVPARRISLDVSEGELADRRVKRKQPEPRYERGYGWMFSRHVQQADQGCDFDFLRTD
                     FGAPVCEPDIF"
BASE COUNT         1247 a         1862 c         1879 g         1171 t
ORIGIN      
        1 gttcgccgat cgatttctct gttgccacgg gctttagcct tttccaccgc tggcggcgcc
       61 gcgataggcc agcgatcgcc cgaacaggat cataacgacg acgatgagct ggcagaaccc
      121 catgatcatg gcgatcgcgg aggccatcga gctgtcgtac tgctcatagg cggcctgata
      181 ggcggcgatg gagatgacgc gagtgtcgcc cgcgggcgct ccgagcagca ccgccgaggg
      241 aaagacggaa aaggtctgta cgaacgacaa gcagagattg atggcgaggc cttgcgcgag
      301 cagaggcagc aggacatgca caaagcgggc ccgcgcctta gcaccgagag tggaagccgc
      361 ctgctccagc gaaggatcga tgccggtcag ataagacagg gttagcagga aggtgaacgg
      421 gaatcccgac aggatcagcg acagcatcac gcccgtataa ttgtgtacca ggcggatctt
      481 tccgagccca atcatgaaca gcactcggtt gaaccagccc tggggcccga gatagatgag
      541 cagaccctcg gcgatcagca cggtgccgag tgtgatcggg atgaccagga tcgcggtcag
      601 caaacggcgg taatccatcc gccgtaccct gagcgcgacc ggcaccgaaa tcagaacatt
      661 gatcagtgtc gccggaatgg cgaggcggaa ggtcgcgccg atcgtgtcat aaaggaacgg
      721 atcagagaaa aacttggtgt aattcgccag ccagttaccc gccttcggct gaaacgacag
      781 cacgaagccg taagcaaacg gataaatgaa cagcaccacg acgaacagca cggccggcaa
      841 agccagcaat gtcacgccgt cgagatcgcg gagcgcaaga cgcacgcgca atggcgtcgg
      901 atcagcgagg gtgccagtcg cccttttgct catgcggtcg cccgcgaata gaccagtacc
      961 cggcccggat ctgccgtgag gcgcacagtc tcacccttgc tcaccctgcg atcggattga
     1021 aaataaagct cggtgccgtc ggcgatccga ccggagcagt aaaagctgtg cccgtggtac
     1081 tgcgccgatt cgacggtcgc cgcgattgag ccagtcgcac tcaccgcaag atcgtcgggt
     1141 cggatcgccg cgaccacctc gccctcattg accgcgtcga cgggtgtcgc cggcaagttg
     1201 gcgccggcga cattgacatg gatcagcttg tcggcgcggg ttgcgcgcac acgaagctgg
     1261 ttgcgataac ccatgaattc ggcgacatcg gcgtgcgcgg gacgtaagta gagctcctcc
     1321 ggcgtgccaa cctggcgcac ctcgcccgcc cgcatgacga cgatgcggtc cgcgagcgac
     1381 agcgcctcct cctggtcgtg cgtgacgtag atagtggccg agccgatcaa ttcatgaatg
     1441 cggcggattt ccgcgcgcat ctcgagccgc agcttggcat ccagattgga taacggctcg
     1501 tccataagca cgagtggcgg ctcgatcaca atggcgcggg cgatggcgac gcgctgctgt
     1561 tgtccacccg aaagctgccc gggcagtttg tcgccctggt cgccgagacg cacgatcgac
     1621 aacgcctcgt cgaccttgcg ctcgatctta tctttggcaa tcccgtgcat gttgaggccg
     1681 aagccgatgt ttcgccttac gctcatgtgc ggaaacagcg cgtaattctg gaacaccata
     1741 ccgaagccac gcgcctctgg cggcaggtga tcgatgcgcg tcgtgtcaag ccagacgctg
     1801 ccgcctgaaa gcgtcagcag gccggccagg caattgagcg cggttgattt gccgcagccg
     1861 gagggcccga gcaaggctat gaactcgccg cggcggacgc tcagcgtgac gcccttgagc
     1921 gcgttgatgc tgccgaacgt gcggctcatg ctttcgagcc gcagctcgcg aaaatcgcgt
     1981 gcagcaatcg ccatgacgag cgcttctccc caagcggcaa cagcttacat aataaaggaa
     2041 gactggcgga ggccgccgcc agtcttctct catgggtagg gcgtcagttc ttcttcttct
     2101 gcgagccaat ctgctcgtcc cagcggctga atgcatacac gagcttatcg ggtagcagcg
     2161 gaatctcctg cggcgctgcc gtgatcgcct gggcatattc gctgcgacca aattcggcga
     2221 tcaccttctg gcttgcttcg ggcgccatcg acaacggcac gtcctttacg gccggaccag
     2281 gatagaaata accgtcatcg taggtcatgg cctgcgccgg tggcgacagc atgtagttga
     2341 ggaaatcgat cagcaccgcg agcttatcgt tggagacccc cttcgggatg cagaggtaat
     2401 gggcatccag aacccaatgg tagcccttga gaaagaacac cttggcttcc ttgggcacca
     2461 cgccgagagc gcggggattg atgtcccaac ccatctgcga tgggatcatg tcgcgagagc
     2521 cttcgccgag ctccttcatg gtcgccccgg tgccggccgg atagtactcg acgtattgcc
     2581 caagctcctt caagtacgac caagtcttgt cccagccgtc cttcggatcg tgcgggtcct
     2641 tgtcaccgag gatgtaggga agccccatca tgaagacgcg ccccggcccg gaattcgccg
     2701 ggcgcgcata catgaagcgg ttgggattag ctttagccca ggcgagcagg tcttcggcgg
     2761 tttgcggcac ctgcttcacg ttatccggca tgtattccat caccggaccc gccggacaga
     2821 acacgatgca aacgccctgg ttcgaggcaa gctcctgcat tctggcggcg ccgggcagat
     2881 agatctcatt gagcttgggc aacttggccg caaattcggt ggcgattgga atccacagct
     2941 tctggtcgac accggccgag agcgcatcta tcccggtcag cacaccgtca atatcgaccc
     3001 ggccggcgtc ctgttgcgcc ttgattttgc ccggcagttc gggagctggt gccttactga
     3061 agtttacctt tgcgaccagt ttcggattgt tgtcgcgata ggcttcgatc gccttctggg
     3121 tgagggcaag gttgccggcc acgtcgacga aattgagcgt gaccggcgat gtgggaagcg
     3181 gcagattttg tgcgaatgcc ggggccgccg cagagcttgc aagacctcct accaccgcac
     3241 ggcgggtcaa cttaatgagc atggtattcc tcccctgagg ttcaacgcga caatggctag
     3301 gttaggtttc cttcagaaaa atttgggaca gacagagcat tgcctcgctc acctatcggc
     3361 ctttcagttt gcgccacacc gaaaaatcct ccttggcctg ttcgtctgtc ggcggataaa
     3421 gcccgataat cgaacggccg tttttgacct cttccgtcac gaaatcctcg aaggcggtca
     3481 tttctaccgc ctcgttggca atctcgtcgg caagatgcgc ggggataacc acgacgccct
     3541 cggcatcgcc aacgaccaca tcgcccggcc acaccggcgc gtcaccgcag gcaatcggta
     3601 cattgatgtc gagcgcctga tggactgtca gattggtcgg tgcggctgga cgatgatgat
     3661 atgcaggaat ggccatgcga gcgatctccg gcgagtcacg aaagcctcca tcggtcacaa
     3721 cgccggcgac gccgcggatc atgagccggg taacgagaat cgatccagct gaagcggcgc
     3781 gggcatcctt gcgactgtcg atgacgaaga ccgcgcctgg cgggcactct tccaccgcct
     3841 tgcgctgcgg atgcgatcga tcctggaaca cagtgattag gttcagatct tcacgcgccg
     3901 ggatgtagcg cagtgtgaag gcctcgccga ccatcgtccc cgccgccggg ttaagcggtg
     3961 tcacatcctg aatgtactgg ttgcgaaagc cccgcttgaa cagcgcggtg cagatcgtcg
     4021 ccgtgctcac tgtcttgagc ttgtcgcgta gcgctacgga aagcatgcct tactctttca
     4081 aaaaatatcc ggctcgcata ccggtgcgcc gaaatcggtc cgtaggaaat cgaagtcgca
     4141 gccctggtcg gcttgctgca catggcgtga gaacatccag ccatagccgc gttcatatcg
     4201 cggttcgggc tgcttgcgct tgacacggcg gtcggcaagc tcgccctcgg acacatcgag
     4261 gctgatgcgg cgtgccggca cgtcgacagt gatcatgtcg ccggtcttaa ccagcccgag
     4321 cggcccgccg acataagatt ccggtgatac atgcagaatg caggcgccgt aactagtgcc
     4381 actcatacgc gcatcggaaa tgcgcaccat gtcgcgcacg ccttgcttga gaagcgtttt
     4441 cggtatcggc agcatacccc actccggcat gcccggcccg ccgagcggtc cggcattgcg
     4501 caacaccaac acgtggtcgc tggtgacgtc gagatcatcg cgatcgatgt tcgccttgag
     4561 atcggggtag gagtcgaaca ccatggccgg gccggaatgt ctcagcagat gcggcgccgc
     4621 ggccgatggc ttcatcacgc agccgttcgg cgccaagttg ccgcgcagta cggcgagtgc
     4681 gccctcggca tagattgggt tgttaagcgg acggataacg tcatcgttat agatttctgc
     4741 ggagtcgagc acgtcaccga gcctgctgcc cgagacgttg acgtgtgaga ggtcgaggaa
     4801 cggcttgagc cgcgacatca acgccggcaa tccgccggca taatagaaat cttccatcaa
     4861 gtatttgtcg ccgctcggcc ggatgttggc gattaccggc acctcgcggc tagcgcgatc
     4921 gaaatcgtcg agattgatac tgtggccggc gcgccgagcc attgccagca aatggatcac
     4981 ggcattggtc gagcaaccca tcgccatcgc cacgacgata gcattgcggt aggcggcctc
     5041 agtttgaatc tttgccggcg tgacgtcttc ccaaaccatt tccacgattc ggcggcccga
     5101 gtccgcacac atgcggatat ggccggaatc gggggcagga atggatgagg caccgggcaa
     5161 tgccatgccg atcgcctccg cgagtgcagt catagtcgcc gcggtgccca tggtcatgca
     5221 cacgccgtag gagcgcgcga tgccgacttc catttcgctc caggccttct cgctgagcgc
     5281 gccagcacgg cgctcgtccc agtatttgaa agcgtcggag cctgacccaa gtacctttcc
     5341 gtgccagttg ccccgcagca tagggccggc cggcacgtag atcgctggca cgccggagct
     5401 ggttgcgccg agcagcagcc cgggcgtggt cttgtcgcag ccgcccatca gtacggcgcc
     5461 gtcgaccggg tgcgagcgga tgagttcttc cgtctccatc gccagcatgt tgcgatagag
     5521 catggttgtt ggcttaacct tactttctga caatgagagc gctggcagtt cgaccgggaa
     5581 accgccggcc tggaagacac cccgcttgat gtcgtcgacg cgctgcttga agtgcgcgtg
     5641 gcattgattg aggtcggacc aggtattgat gatgccgatg acgggccgac caacatagtc
     5701 ggcaccggaa tagcccatct ggctcatgcg ggagcgatgg ccaaacgaac gaaaatcgtc
     5761 cggcagaaac caacgcgcgc tgcgcagctc cttcggctgc ttgcgtgaca tccgctacac
     5821 caaaccgttc cgccgcaggc ctgcgcgcga gtttgcgtca attacagcag ggatttgccg
     5881 ccggtggaat ggggttcagg cgttccggtg gaatggggtt caggcgttag tctgcacggc
     5941 agcaatcctg agccgcccac gtccgctttg ggtcaaaagc agacatcagt gcatgtccgc
     6001 gtgatgtccg ctttaccccc aaaagcggac attctgcgct gcagcagaaa tcgtacgatt
     6061 tcgtatccaa agggctgctg agttttgtgg gtttctgaac cgcccgcaga acggcaagaa
     6121 gcgacggctc gctgagcaaa ccgtcgcctc cctgtcagt
//
LOCUS       DATLJT010000568         6550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1412272,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000568 DATLJT010000000
VERSION     DATLJT010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412272"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VM910_13125"
     CDS             complement(<1..419)
                     /locus_tag="VM910_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HVI63511.1"
                     /translation="MESSSKFDIVVYGASGFTGQLVAEYLAVHYKGDKQLRWAMAGRS
                     LDKLASVRDAIGAPKETPLIAADASDVASLKAMVEQTKSVISTVGPYQFYGNDLVAIC
                     AASGTDYLDLCGEPVWMRQMIDKHEAAAKASGARIVFS"
     gene            complement(521..1306)
                     /locus_tag="VM910_13130"
     CDS             complement(521..1306)
                     /locus_tag="VM910_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HVI63512.1"
                     /translation="MQQQTPPEQFDLAGIVADLNALLRLKTTVTGIKMFSRVEDMLAI
                     EKIRRPNAVHTTDQIVSMASRLGWTVGVTADDLVGAQCRAVIGLAPQDEKWLAGENYV
                     GVWHGTAEDARKRQEALDVVPFGRYQAMAVSPLTSGRLNPPDICLVYATPGQMIILIN
                     GLQYTGYKKFEWGVVGETACADSWGRALKTGEPSLSLPCFAERRYGGVPDEEMLMALK
                     PSHLVKAIQGMKQLAKNGLRYPIAPYGIQSDVRAGMGVSYGKK"
     gene            complement(1401..2657)
                     /locus_tag="VM910_13135"
     CDS             complement(1401..2657)
                     /locus_tag="VM910_13135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HVI63513.1"
                     /translation="MTSEVVRTASRRRFLQYLAASPLVASTGLPAYAGDAPLKLPDPM
                     IWAPRGLEPIKSPKEAINVFDFEPAAFKNVPPAHFGYMASGIDDEVTLRANREGFLKF
                     QLRPRRLNDVSKVDMSVDIFGSKYDSPVFISPTGGNQFFHEDGEVAVAKAARSGNHLH
                     ILSTSSNFSVEDVTKARGAPIWFQLYASPSWEVAQALIRKAEAAGCPVLMVTVDRVAG
                     RNQETLFRLMRTDSRECSSCHDRSSFAARAARRHNYDGIDLTKITGSGESSNLTWDTV
                     KRMRDTTRMKIVLKGIVTPEDAELSVKNGMDGILLSNHGGRGEDNGRSTIDALPEIIV
                     AANGKMPVFIDGGFRRGTDVVKALALGATAVGVGRPYLWGLGAFGEEGVTRVLEIMRT
                     ETRVAMQQCGVRSISELNPNFVRRAS"
     gene            complement(2795..3601)
                     /locus_tag="VM910_13140"
     CDS             complement(2795..3601)
                     /locus_tag="VM910_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HVI63514.1"
                     /translation="MFFVLSKTLGVMALPTNLLIGLGILGAVLLATRFASWGRKLLVA
                     AILLLAVFGFTPLGNFFLYPLESRFPPWDPARGAPDGIVVLGGAIETDASEVHGETVF
                     NASAGRMISAAELAHRYPNARILFTGGNASLVGDDSRKEADFVTAMFEGLGISKDRLM
                     LERLSRNTFENAEFSKALASPKPGERWLLVTSAFHMPRSVGIFRKAGFAVEPYATDWR
                     LAGRADLTRFTAFSVDGLQHTDLALREWMGLLAYWITGKTSELFPGPANN"
     gene            3670..4611
                     /locus_tag="VM910_13145"
     CDS             3670..4611
                     /locus_tag="VM910_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HVI63515.1"
                     /translation="MTHHQLKSSPETCHWGFFEAKLKPVLTIESGDEVTVDTISGGPE
                     VVPDKTRFHVPPELFEVHAKNERMVPGHILTGPIAVRGAEPGDVLEVDILDVRLRQDW
                     GYNLIRPLAGTLPDDFHEPRILNIPLDVKRMVGRLPWGLDLPLAPFFGVMGVAPPPAW
                     GRITSLIPRAMGGNLDNKELGAGSKLYLPVFVPGALFSCGDGHGVQGDGEVCVTAIET
                     ALQGRFRLTLRRDLRLDYPRAETPSHYMTMAMDPDLDQCVVRALRDMIVLLGEKRNLS
                     REDAYTLCSLAADLRVTQTVNGSKGIHCMIAKSVVHG"
     gene            4713..5189
                     /locus_tag="VM910_13150"
     CDS             4713..5189
                     /locus_tag="VM910_13150"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HVI63516.1"
                     /translation="MADPDEVAISHLQRRKIEARVLLPLLQTLKEKLGEDVTREVLDA
                     TITRLAVSDGTTWAASYGQTIAGLRRIALELWAGSGALDIEMIGESADHLDFNVTRCR
                     YAEFYRDQGLTDVGSRIHCNRDLAMITGFDSEMELNRSQTIMQGAPCCDFRFRRKA"
     gene            complement(5217..6041)
                     /locus_tag="VM910_13155"
     CDS             complement(5217..6041)
                     /locus_tag="VM910_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI63517.1"
                     /translation="MIAYHVVTSGRCFASLDGGQAVPVEAGEVVMFTNADPHVMSSST
                     GMRAEPPSADMIEIADAGLLPFHVNLMNGGAASARLVCGYLACDAKPFNPLLAALPPM
                     LKAGDPRRNDTSWLGQFIHFAVAEVAEKRAGSESVLTKLSELMFIDVVRRYIEALPPQ
                     DTGWLAGLRDPAVGKALALIHARPSFNWTIEGLARQCGTSRTVMAERFVQFVGVPPMH
                     YLAKWRMQVASEMLKRGNANMAIIAAEIGYESEASFSRAFKKMMGVPPSAWRQGTR"
     gene            6212..>6550
                     /locus_tag="VM910_13160"
     CDS             6212..>6550
                     /locus_tag="VM910_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63518.1"
                     /translation="MPDRPQKITFGEMRDMGVRGLLIYCADYRCSHSIAISADGWPDD
                     VRLSDIEARLCARPASGLSGGSIFDRADDRRENGSAGPACDQLGNDATNAQIARLRCC
                     HNRGQRKSYDL"
BASE COUNT         1258 a         2080 c         1970 g         1242 t
ORIGIN      
        1 gagaagacga tccgcgcgcc gctcgccttg gctgctgcct cgtgcttgtc gatcatctgg
       61 cgcatccaga ccggctcgcc gcagaggtcg agataatcgg tgccggaagc cgcgcagatc
      121 gcgacaagat cgttgccgta gaactgatac ggaccgaccg tcgagatgac ggatttggtc
      181 tgctcgacca tcgccttcag cgatgcgacg tcgcttgcat cggccgcgat cagcggcgtc
      241 tccttcggcg cgccaatggc gtcgcggacg gacgcgagct tgtcgaggct gcggccggcc
      301 atcgcccacc gcaactgctt gtcgcccttg taatgcacgg cgagatattc ggcgacgagc
      361 tgaccggtga aaccggaggc gccatagacc acgatgtcga acttcgacga agattccatt
      421 tggaattccc aagtttttca tcattgcgaa gagcgaaggg acgaagcaat ccggttgttt
      481 ttcccggcct ggtgctacgc ggcgctggca atgacggagc ttacttcttt ccgtaactta
      541 cgcccatacc ggcgcgcacg tcgctttgga tcccatacgg cgcgatcgga tagcgcaaac
      601 cgtttttggc gagttgcttc atcccctgaa tcgccttcac aaggtgcgaa ggtttcagcg
      661 ccattagcat ctcctcatcg ggaacgccac catagcgccg ctcggcgaag cacggcagcg
      721 acaggctcgg ctcgccggtc ttcagcgccc tgccccacga atccgcgcag gccgtttcgc
      781 cgaccacgcc ccactcgaat ttcttgtagc cggtatattg cagcccattg atcaggatga
      841 tcatctgccc cggcgtcgca tacaccaggc aaatatccgg cgggttgagc cgaccgcttg
      901 tgagcgggct caccgccatc gcctgatagc ggccgaacgg cacgacgtcg agcgcctcct
      961 ggcgcttgcg tgcatcctcc gccgtgccat gccagacgcc gacatagttc tcgcctgcga
     1021 gccatttctc gtcctgcggg gcaagcccga tcacggcgcg gcactgcgca cccacgagat
     1081 cgtcggcggt gacgcccacc gtccaaccga ggcgcgaagc catgctgaca atctggtcgg
     1141 tggtgtggac cgcattcggg cggcgaatct tctcgattgc gagcatatcc tcgacgcgcg
     1201 aaaacatctt gatgccggtc accgtcgtct tcagacgcaa aagcgcgttc agatcggcga
     1261 cgatgccggc cagatcgaac tgttccggtg gtgtctgctg ttgcatggcc tttctcccga
     1321 gctgcggctt gcgcaccctt tccttcgatc ctacccaaaa tgtttggcgg gcgcgactac
     1381 cgcatcccgt ctcttgacgc ctaactcgcc cttcgcacga aattcgggtt gagctcggaa
     1441 atcgaacgca cgccgcattg ttgcatcgcg acccgcgttt cggtccgcat gatttccagc
     1501 acccgcgtga cgccctcctc accgaacgcg cccaatcccc agagataggg ccgccccacc
     1561 ccgaccgccg tcgcaccgag cgcgagcgcc ttcaccacat cggtaccgcg gcgaaaaccg
     1621 ccgtcgataa acaccggcat tttgccgttc gccgccacga tgatctccgg caatgcatcg
     1681 atggtggagc ggccattgtc ctcgccgcgg ccgccatgat tggaaagcag aatgccgtcc
     1741 atgccgttct tgacggaaag ctctgcgtct tccggcgtga cgatgccttt gagcacgatc
     1801 ttcatgcgtg tcgtgtcgcg catgcgcttc acggtgtccc aggtcagatt gctggactcg
     1861 ccgctcccgg tgattttcgt cagatcgatc ccgtcataat tgtgccggcg cgccgcgcgc
     1921 gcggcgaagc tcgagcgatc gtgacaggac gagcattcgc gcgagtcggt tcgcatgagc
     1981 cgaaacagcg tttcctgatt gcgccccgcg acgcgatcca cggtgaccat cagcaccggg
     2041 cagccggccg cttcggcctt cctgatcagc gcctgcgcaa cctcccagct tggcgacgcg
     2101 tagagctgga accagatcgg cgcgccgcgg gccttggtga cgtcttccac ggaaaaattc
     2161 gacgaggtcg acaggatatg caaatggttg cccgatcgcg ccgctttggc gacggcgacc
     2221 tcgccgtctt cgtggaaaaa ctggttgccg ccggtcggcg agatgaacac cggtgaatcg
     2281 tacttgctgc cgaaaatgtc gacgctcatg tcgaccttcg agacgtcatt gagccggcgc
     2341 ggacgaagct ggaatttcaa aaacccctca cggttggcgc gcagcgtcac ctcgtcgtcg
     2401 atgccggagg ccatgtagcc gaaatgcgcc ggcggtacgt ttttgaacgc ggccggctcg
     2461 aagtcgaaca cgttgatcgc ctctttcggg cttttgatcg gctcaaggcc ccgcggcgcc
     2521 cagatcatcg ggtccggcaa tttcaacggt gcgtcaccgg cgtaagccgg cagtccggtc
     2581 gatgcgacca gaggacttgc ggcgagatat tgcaagaacc ggcggcggct cgctgtgcga
     2641 acgacttcgg atgtcatttc attcctccct gccattgttc ttggttgttc aaagagggat
     2701 cgtagcattt gtttcaagcg cggaggagcg gccgcccatt tgcgggcccc gtttgccgag
     2761 cccattttcg ccggcaccgc aggggtccca tccgctaatt atttgctggt cccggaaaca
     2821 gctcgctggt cttgccggtg atccagtagg ccagaagccc catccattcg cgcagcgcca
     2881 ggtccgtatg ttgcaggccg tcgacggaaa acgcagtgaa gcgggtgaga tcggcgcgcc
     2941 cggcaagacg ccagtcggtc gcgtaaggct cgaccgcaaa acccgctttg cggaagatcc
     3001 cgaccgaccg cggcatgtgg aaggcggagg tgacgagcag ccagcgttca ccgggttttg
     3061 gcgaggcaag cgctttggaa aattcggcgt tttcgaacgt attgcgcgac agtcgctcca
     3121 gcatcagacg gtctttcgat attccgagtc cctcgaacat tgcagtgacg aagtcggcct
     3181 ccttgcgcga gtcgtccccg acaaggctgg cgttgccgcc agtaaagagg atgcgtgcat
     3241 tgggatagcg atgcgcgagc tcggcggcgc tgatcatgcg gccagccgag gcgttgaaga
     3301 ccgtttcgcc atgcacctcg gacgcgtcgg tttcaatcgc cccgcccagc acgacgatgc
     3361 cgtcgggcgc accgcgcgca ggatcccagg gtggaaatcg gctctcgagc ggatacaaga
     3421 aaaaattccc gagcggggta aatccgaaaa ccgctagcag caggatcgcg gcgacgagga
     3481 gcttgcggcc ccatgacgca aaccgcgtcg caagcagcac ggcgccgaga atgccgagcc
     3541 caatcaacag attcgtcggc agcgccatga cgccgagcgt tttcgacagc acgaaaaaca
     3601 agggaggtcc ccaaaatatc tgctacagcg tcatacgccg tctttcccat gcattccaaa
     3661 gactttctca tgacgcatca tcaactcaag tccagccccg aaacctgcca ttggggcttc
     3721 ttcgaagcga aactgaagcc ggtgctgacc atcgagagcg gcgacgaggt caccgtcgac
     3781 accatctcgg gcggccccga ggtggtgccg gacaagacca ggtttcacgt gccgcccgag
     3841 cttttcgagg tgcatgcgaa gaacgagcgc atggtgccgg gccacatcct gaccggcccg
     3901 attgcggttc gcggcgcaga gcccggcgat gtgctggaag tcgacatcct cgacgtcagg
     3961 ctgaggcagg actggggcta taacctcatc cggccgctcg ccggcaccct gcccgacgac
     4021 ttccacgagc cgcgcattct caacatcccg ctcgacgtca aacgcatggt cggccgattg
     4081 ccctgggggc tcgacctgcc gctcgcgccg tttttcggcg tgatgggcgt tgcgccaccg
     4141 cccgcctggg gtcgcatcac ctcgctcatt ccgcgtgcca tgggcggcaa tctcgacaac
     4201 aaggaattgg gcgcaggcag caagctctat ctgccggtgt tcgtgccggg tgcattgttt
     4261 tcctgcggcg acggccatgg cgtgcagggc gacggcgagg tctgcgtcac cgcgatcgag
     4321 acggcgctgc agggacgttt ccgcctcacg ctgcgaaggg atctcaggct cgattatcct
     4381 cgcgccgaga cgccgtcgca ctacatgacc atggcgatgg accccgatct cgaccaatgc
     4441 gtggtgcgcg cgctgcgcga catgatcgtt ctgttgggcg aaaagcggaa tctgtcgcgc
     4501 gaagacgcct atacgctatg cagccttgcc gccgatctca gggtgacgca gacggtcaac
     4561 ggctccaagg gcattcactg catgatcgca aagagcgtcg tgcacgggtg atggtagact
     4621 gcgacggtcc gacggtcagg cgtttctggg caatcgcacg agagcccaac gcagcctgaa
     4681 agcatgggca acaaacgaga taggatatgc acatggccga cccagatgaa gttgccatca
     4741 gtcacttgca gcgccgcaaa atcgaagcgc gcgtgctcct gccgcttctt caaacgctga
     4801 aagagaaact cggagaggat gtcacgcgtg aagtcctcga tgcaacgatc accaggctcg
     4861 ccgtctcgga tggaacgaca tgggccgcct cctacggaca gaccatcgcc ggcctgcgca
     4921 gaatagcgct cgagctatgg gcgggcagcg gtgcgctcga cattgagatg atcggcgaat
     4981 ctgccgacca tctcgatttc aacgtgaccc gctgccgata cgccgaattt tacagggatc
     5041 aggggctcac cgatgtcggt tcccggattc actgtaaccg cgatctcgca atgatcaccg
     5101 gatttgacag cgagatggaa ctcaatcgaa gccagacgat catgcagggc gcgccctgct
     5161 gtgacttccg atttcgcaga aaggcctgat caggcgagag tgaaaactcc gacctgttac
     5221 cgcgtcccct gccgccaagc cgacggcggc acacccatca tcttcttgaa ggcgcggctg
     5281 aaggaggcct cggattcgta gccgatctcg gcggctatga tcgccatatt ggcattgccg
     5341 cgcttgagca tttccgacgc cacctgcatc cgccactttg caagataatg catcggcgga
     5401 acgccgacga attggacaaa gcgctcggcc atcaccgtgc gggaggtgcc gcattggcgc
     5461 gccagcccct cgatggtcca gttgaacgaa ggtcgtgcgt ggatcagcgc cagcgccttg
     5521 ccgactgcgg gatcgcgcag ccccgcaagc cagccagtat cctgcggggg cagcgcctcg
     5581 atgtaacggc gcaccacgtc gatgaacatc agctcgctga gtttcgtcag cacgctctcg
     5641 ctgccggcgc gcttctcggc gacctcggcg accgcaaagt gaatgaactg cccaagccaa
     5701 ctcgtgtcgt tgcgacgggg atcgccggcc ttcagcatcg gcggcagcgc cgccagcagc
     5761 gggttgaacg gcttcgcatc gcaggcgaga tagccgcata cgagcctcgc cgacgccgct
     5821 ccgccgttca tcaaattgac gtggaagggc agcaggccgg cgtcggcaat ctcgatcatg
     5881 tcggcgctcg gcggttcggc gcgcattcct gtgctgctcg acatcacgtg cgggtcggcg
     5941 ttggtgaaca tgacgacctc gccagcctcg accggcacgg cctgcccgcc atcgaggctc
     6001 gcgaagcagc gacccgacgt cacgacgtga taagcgatca ggtgaaacgg tttaacctgc
     6061 ctatcagcat cttgatattg ctagatattt ctagccatag gttctgctat cttctcaggg
     6121 gtaaccagcg gggtatcatt ttgatgtgac ccacaaataa aggcgcgccg tggtgacttc
     6181 cctcctggcg ctatgggctt aatcatgggc aatgcctgac cggccgcaaa agatcacgtt
     6241 tggcgaaatg cgcgacatgg gcgtgcgcgg cctgctgatc tattgcgccg actaccgctg
     6301 cagccactcc atcgcgatca gcgccgatgg ttggcccgat gatgtcaggc tgtccgatat
     6361 cgaggcgcgg ttgtgtgcca ggcctgcgtc tgggttaagc ggcggctcga tttttgatcg
     6421 cgccgatgat cgccgtgaga atggctccgc cggccccgcc tgcgaccaat tgggaaatga
     6481 tgctaccaat gctcaaattg cccgactccg ctgctgccat aaccgagggc agcgcaagag
     6541 ttacgatctg
//
LOCUS       DATLJT010000569         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1642501,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000569 DATLJT010000000
VERSION     DATLJT010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642501"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="oxlT"
                     /locus_tag="VM910_13165"
     CDS             complement(<1..1188)
                     /gene="oxlT"
                     /locus_tag="VM910_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HVI63519.1"
                     /translation="MTAAEATRWSQLIFGIICMVMISNLQYGWTLFVDPINEKYHWDR
                     AAIQVAFTIFALTDTWLVPFEGYLIDRFGPRIVVSVSGVLVAIAWVINSVADSLFLLY
                     LGAAIGGIGAGAIYGGCVGNALKWFTDRRGLAAGLTAAGFGAGSALTLIPIANMIQSS
                     GYESAFLWFGLGQGIVVVLIATLLRAPSPSELPVPTAPAVEQTRRDYRPVEVLASAPF
                     WVMYAMFVMVSTGGLMATAQLAPIAKDFKIDTVPVSILGLTLPALQFALALDRVLNGV
                     TRPLFGWVSDRIGRENTMFIAFLLEGIGIYALLHFASDPFLFVILSGVVFFAWGEIYS
                     LFPATCTDIYGRKFATMNYGMLYTAKGTASLLVPFGNVLTSWTGSWTAVFVIAAILNI
                     VAAI"
     gene            complement(1406..2233)
                     /locus_tag="VM910_13170"
     CDS             complement(1406..2233)
                     /locus_tag="VM910_13170"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63520.1"
                     /translation="MEKLGWTAGRNVLIEYRWAAGSEERGQALAKELVALKPDALVAY
                     GTPMLEATRRATRTIPIVFTMVTDPVGQGFVVTLARPGGNATGFTSFEVSIGGKWLEL
                     IKEISPRVKRAELMFNPVTAPYGTSYLHSAESAAASFGVDVTGAPIRDDADIERIITA
                     LGGAQSGSLIVVPDTFTDVRRDLIIELIARHRLPAVYPYTHFAESGGLMAYGPDPAHM
                     FGQTADYVDRILRGAKPADLPVQQPNKFELVVNLKTANVLGFAVPPMLMARADRIIE"
     gene            complement(2194..2448)
                     /locus_tag="VM910_13175"
     CDS             complement(2194..2448)
                     /locus_tag="VM910_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63521.1"
                     /translation="MRKISLVGVAAALILAGVGSWVGSTTLGGAVIGWSGVSIAEEAN
                     GPRRLGVLMRIAEDDPSAQTDFKRSSVDWKSWDGRPAAMS"
BASE COUNT          600 a          878 c          839 g          525 t
ORIGIN      
        1 gatggccgcg acgatgttga ggatcgccgc gatcacgaac accgcagtcc aactgccggt
       61 ccagctcgtc agcacgttgc cgaatggcac cagcaatgac gccgtcccct tggccgtgta
      121 cagcatgcca tagttcatgg tggcgaattt gcggccatag atgtcggtgc acgtggctgg
      181 gaacagcgaa tagatctcgc cccaggcgaa gaacaccact ccggacagga tcacgaacaa
      241 gaatggatcg cttgcaaaat ggagcagcgc atagatcccg attccttcga gcaggaacgc
      301 gatgaacatc gtgttctcac gaccgatccg gtcggagacc cagccgaaga gcggtcgcgt
      361 caccccgttg agcacgcggt cgagcgccag cgcgaattgc agcgctggca gcgtcagccc
      421 caggatcgag accgggacag tgtcgatctt gaaatccttg gcgatggggg cgagctgtgc
      481 cgttgccatc aggccgccgg tgctgaccat cacgaacatg gcgtacatta cccagaacgg
      541 cgcgctcgca agcacctcga ctgggcgata gtcgcgccgg gtctgctcca ccgccggggc
      601 ggtcggtacc ggaagttcgc tcggacttgg cgcgcgcagc aacgtcgcaa tgagaacaac
      661 cacgatgccc tgtccgagcc cgaaccaaag gaacgcggat tcgtaaccgc tcgactggat
      721 catgttggct ataggaatta gggtcagcgc cgaaccggcg ccaaaaccgg cggcggtcaa
      781 ccccgcggcg agcccgcgcc ggtcggtaaa ccacttgagc gcgttgccta cgcaaccgcc
      841 gtagatcgcg ccggcgccga tgccccctat cgcggcgccg agataaagca gaaagaggga
      901 gtcggctacc gagttaatga cccaggcgat cgcgaccagc acgccgctaa cgctgaccac
      961 gatgcgcgga ccgaacctat cgatcaagta accttcgaag ggcaccagcc aagtgtcggt
     1021 cagcgcgaag attgtgaagg ccacttggat cgctgcgcgg tcccagtgat acttctcatt
     1081 gatcggatca acgaacaggg tccagccata ttgcagattt gagatcatca ccatgcaaat
     1141 gatgccgaag atcagctggc tccaccgcgt agcctccgcc gcggtcatct gcggcgaaat
     1201 cgccccagag gacaggatga aagtgatgcg acgcatgggg gcctcgcttt gggtagggct
     1261 cttgcggcca aaacgacgat cttgcgcgaa cgtcgtgatt gcattcgcat ttcgccggcg
     1321 attttttgtc gtgtcagtta gctacggtga acgaggccgt cgccaaggtc cttgccgcgt
     1381 agcattgcca gaagtaaggc aaatcctatt cgatgatccg gtcagcgcga gccatcagca
     1441 tgggcggtac cgcgaaaccg agcacatttg ccgtcttgag attgacgacc agctcgaatt
     1501 tgttcggctg ctgcaccggg aggtcggcgg gctttgcgcc ccgaaggata cggtcgacat
     1561 agtctgccgt ttgcccgaac atgtgggccg gatcaggacc gtaagccatc agaccgccgc
     1621 tttcggcgaa atgtgtgtag ggatatacgg cgggcaagcg atgccgagct atcaactcga
     1681 tgatcaagtc acgacgaaca tcagtgaagg tgtcgggaac aacgatcagg ctgccactct
     1741 gcgctccccc aagtgccgtg ataatgcgct cgatgtcagc gtcgtcgcgg atgggtgcgc
     1801 cggttacatc aaccccgaaa gacgcggcag ccgactctgc tgagtgcaag taggacgtac
     1861 cataaggcgc ggttaccgga ttaaacatca attcggcccg tttgacccta ggcgagattt
     1921 ccttaatcag ttccagccat ttcccgccga tcgagacttc gaagctcgtg aatccggttg
     1981 cattaccgcc cggcctcgcc agggtcacga cgaagccttg gccgaccgga tcggtgacca
     2041 ttgtgaacac aatcggtatg gtcctggttg cccgccgagt ggcctctaac atcggtgtgc
     2101 cgtaggctac gagagcgtcg ggtttgagcg cgactagctc tttcgccaat gcctggccgc
     2161 gctcctcgga gccggcagcc caacgatatt cgatcaggac attgcggccg gccgtccatc
     2221 ccagcttttc cagtccacgc tggaacgttt gaagtcagtc tgcgcactgg gatcatcttc
     2281 ggcgattcgc atcagcacac cgagtcgccg cggcccattg gcctcctcgg caatcgacac
     2341 gcccgaccac ccgatcactg cgccgcccag cgtggtcgac cccacccatg atccgacgcc
     2401 ggccaagatt aatgcggcgg caacaccgac tagactgatc ttgcgcatcc gggttctccc
     2461 gttcaatcaa gccaggcgcc ggacggcgct gccgcgttcg tcaacccccc tgcagcgttg
     2521 gcctttgatc catggcgccg tccatctctt ttagggatgg tacgtatcgc gccaatgtcg
     2581 cgacggcggg caaagtgtat cgtcagtatc gctggcacac tcggcctgcc acggaaggcg
     2641 aacctaaagt tcaactgacc ggcatcggtc gcgacgccgt ggccggcgca aggatttctt
     2701 aaaatgtaaa cctagccgga cgcccggcgt acatgcgctg gcgacacctc atcttcgagc
     2761 gacgggtcgc gaacgatgat cttgtccagg taaagctgcc ggatctccgc ctcgctcatc
     2821 tccaaaatgc cgcgcaggat gt
//
LOCUS       DATLJT010000570         6146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_493798,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000570 DATLJT010000000
VERSION     DATLJT010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493798"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VM910_13180"
     CDS             <1..330
                     /locus_tag="VM910_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI63522.1"
                     /translation="LGDGCFQMTCGEVATAKRLGITLPVVVLDDRWLALIKVKQIRRQ
                     FPLYGTELQAEAYREPPAHYFGVPARAANSADALEAAVKAALAAKGPTVIEAVVDSEH
                     YIDTVYD"
     gene            complement(391..798)
                     /locus_tag="VM910_13185"
     CDS             complement(391..798)
                     /locus_tag="VM910_13185"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI63523.1"
                     /translation="MPFYHCLAPAGVFTQPMKQEIVDEITRIHCDATGALALFIQVQF
                     EDVDSSNVFQNRKPSSAVRLHARIRAGRPPEVRLKMLSDYTAMLHRITKVPVADIMVA
                     FIETPFENVMEGGVRLPAPGEEAEWKAQFADAS"
     gene            complement(921..2183)
                     /locus_tag="VM910_13190"
     CDS             complement(921..2183)
                     /locus_tag="VM910_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI63524.1"
                     /translation="MLNSAERQEMVAPRRDRQAFAGAAIALAVLFMGNNLPSALYGVM
                     RAAFGYSSLTQTLLYAAAVAVILPGLAIFGPLSDVIGRRVLVVAGLLSFGAGDVLFVV
                     AHDTAGLFAARVVQGLGMALATAAATATLSDNAGMLFLDHERAQRIAALTATTCVTGG
                     LAFGPLLGGILAQYGPAQLKLSFIIHLALVAIALAAARRLPSKPATAIGPWRPVSFRV
                     PVSVRRTFALIAASAFLAWTVFGVFSAVIPSFLSDLLDTKNVALASGALALMIATSAL
                     AQFGAHRLHAVSAQLLGLGTLAVGLALLVAATVIRDPILAVLAMLGSGVGHGFIFVGD
                     MSEISMATPAEERGAVIGTIYLVNYIGLGGPVIGVGMLSLSYGLLAATRVAAAVIAAL
                     CVLLIPLVVGAANTPAGAAADGRFRPRC"
     gene            2580..2885
                     /locus_tag="VM910_13195"
     CDS             2580..2885
                     /locus_tag="VM910_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63525.1"
                     /translation="MNELLDWLRLQKGGVRTYTQFCQKALALRASQPEHAALARLLAD
                     LAGRFADAYDGEPLFLDVAERALNRLTRLVERAVRVSSAGLPEQVALLNEIGLAELT"
     gene            3077..3469
                     /locus_tag="VM910_13200"
     CDS             3077..3469
                     /locus_tag="VM910_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63526.1"
                     /translation="MPYLHLDLPGSYPVEVKRELATRLCKLYAEVMETQLWRPNVGIA
                     ELGKHNLYHLGSDGLESITMVMVEFRRGRSAERRLALGRAIVDICVELLGVPRRTVMV
                     EFTPHRGEEILRDGNWVADWTPAEAVTG"
     gene            complement(3509..4450)
                     /locus_tag="VM910_13205"
     CDS             complement(3509..4450)
                     /locus_tag="VM910_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI63527.1"
                     /translation="MASSAGDMAIFQRVAERGSFAGAAEDVGLSPSAVAKLITRLEQR
                     LGVRLINRTTRHLALTAEGEIYLDRVREILGAIEAAESEVASARASPRGHLRVHTLPV
                     IAAHDLAPAVLDFLARYPHITLDFMVTNRVVDLVGENVDVSLRMGPLEDSGLISRKIV
                     DLRRVVCASPSYLARHARPVEPSDLVRHACLTLSRNPGSASWPFRVNGKLIRIDVKGP
                     VSADSAEMLLQLAIAGGGILRLSEHVVARSIHARLLQPLLQDAQDPEIYPLYALLPPG
                     RHQAPKVRAFIDFLIERLGSAPWRTGSGANHGRKSRR"
     gene            4537..5001
                     /locus_tag="VM910_13210"
     CDS             4537..5001
                     /locus_tag="VM910_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI63528.1"
                     /translation="MPPELANSVKAGGDFNAEEDQAMPYLQLDVPSHYPVDVKRGLAR
                     RLGEKYARIMQTTPELVTVAFRELGEGGVWCCNAGEPEPAALLACDIRRGRPPEQRAE
                     LAQALIDACVETLGLPSDRLVVEFTQHTADEMYRAGRGWAAEWTPAEATSTV"
     gene            5042..5512
                     /locus_tag="VM910_13215"
     CDS             5042..5512
                     /locus_tag="VM910_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI63529.1"
                     /translation="MAITIKVNGVDRTVDVDGDTPLLWVLRDVLGMTGTKFGCGMALC
                     GACTVHIDGVATRSCITPIDSIGASAVTTIEAIGATAAGAAIQKAWLDREVVQCGYCQ
                     SGQIMSASALLATNPHPTDSDIDNAMSGNICRCGTYLRIREAIKQAAQSSGQGG"
     gene            5515..>6146
                     /locus_tag="VM910_13220"
     CDS             5515..>6146
                     /locus_tag="VM910_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI63530.1"
                     /translation="MRVDHLSSFADGSARSSSASGVSRRTLLQAGAAAGGGLLLSLRL
                     PFASGDAEAAGADGFAPNAFIRIGSDGQIVLTMPYVEMGQGTYTSIPMLIAEELEVDL
                     KQVRLEHAPPNEKLYGNPLIGGIQATGNSNAVRAAWQPLRQAGATARTMLVSAAAKRW
                     NADAGSCRAHSGEVLHPPTGRRAKYGELAADAARVPVPKNVALKRPQDFKL"
BASE COUNT         1238 a         1913 c         1933 g         1062 t
ORIGIN      
        1 ctgggggacg gctgcttcca gatgacgtgc ggcgaggtcg cgaccgccaa gcggctcggc
       61 atcacgcttc ccgtcgtcgt gctcgacgac cgctggctcg cgcttatcaa ggtaaagcag
      121 atccgccgcc agttcccgct ctacggcacc gagctgcagg cggaagctta ccgcgaaccg
      181 ccggcgcatt atttcggcgt gccggcgcgc gccgccaaca gtgccgacgc gctcgaggcc
      241 gcggtcaaag cggcgctggc agcaaaggga ccgacggtga tcgaggccgt ggtcgattcc
      301 gagcactaca tcgacacggt ttacgattga cccactcctc tacgcgccgg gccgcgacct
      361 cgacgaatag gtcgccgtat ccaaagccac ctatgaggcg tcagcgaact gagccttcca
      421 ctccgcctct tcgccgggcg caggcagcct tacgccgccc tccatgacgt tctcgaatgg
      481 ggtttcgata aaggcgacca tgatatcggc cacgggtacc ttcgtgatcc gatgaagcat
      541 ggccgtgtaa tcgctgagca ttttcaaccg gacctccggt ggccgaccgg cgcggatgcg
      601 tgcgtgaagt cggacagcag acgacggttt ccggttttga aagacgttgc tggaatcgac
      661 atcctcgaac tgaacttgga tgaacagagc gagcgcaccc gtcgcgtcgc agtggatgcg
      721 tgtaatttcg tcgacgattt cctgcttcat cggctgggtg aaaacgcccg ctggcgcgag
      781 gcaatgatag aacggcattt gtgatctcct tagtgtcgcc tcggcgaggc ttgcatgacg
      841 ttaccggtca gccaactagc tctatgccgc cgatatagat gctcattttt gtaccgataa
      901 tatggaaggc cgcggaactg ctaacatcgt ggtctgaatc ggccgtcggc tgctgccccg
      961 gcaggcgtgt tcgcggctcc gaccaccaac gggatgagca gcacgcaaag tgcagcgatg
     1021 accgctgccg ctacgcgggt ggctgccaac aaaccgtagg acagcgacag cattcccacg
     1081 ccgatcacgg ggccgccgag tccaatgtag ttgacaaggt agatagtgcc gatgactgca
     1141 cctcgctcct cggccggcgt agccatgctg atctcactca tatcgccgac aaaaatgaag
     1201 ccgtgcccta cgccggagcc gagcatagcg agcacggcga ggatcggatc gcggatgacc
     1261 gttgcggcca ccaacaacgc tagtccaacc gcgagtgtgc cgaggcccag caattgggcc
     1321 gacaccgcat ggaggcgatg cgcgccgaac tgcgcaagcg ccgaagtcgc gatcatcaat
     1381 gccaaggctc ccgatgcgag ggcaacgttc ttggtgtcca acagatcgct gagaaaggac
     1441 ggaatcacag ccgagaatac gccgaacacg gtccaggcca ggaacgcgga ggcggcgata
     1501 agagcgaagg tccggcggac ggacacggga acgcggaacg ataccgggcg ccaggggccg
     1561 atggccgttg ccggcttgct gggcaatcgc cgcgcagcgg caagagcgat cgcgaccagc
     1621 gccagatgga taataaagga gagcttgagc tgggcgggac catattgcgc caagatacct
     1681 ccgagcagcg ggccgaatgc caatccgccg gtcacacatg tggtcgccgt cagtgccgca
     1741 atccgctgcg cgcgttcgtg gtccagaaac agcatgccag cgttgtcgct caaggtggcc
     1801 gtcgcggcgg cagtggccaa agccattccc aagccctgta ccactcgcgc agcgaacagc
     1861 ccggccgtgt cgtgcgctac gacgaacaag acgtcgccgg cgccgaacga gagaaggccc
     1921 gcgacgacaa gcacgcgccg cccaatgaca tccgataacg gcccgaaaat agcgagtcct
     1981 ggcaagatga ccgccaccgc ggcggcgtat agcagcgtct gggttagcga cgaatatccg
     2041 aacgctgccc gcattactcc gtatagcgcg ctgggcaagt tgttgcccat gaatagcact
     2101 gcaagtgcaa tcgcggctcc tgcaaacgct tgcctgtcac gtcgcggcgc gaccatttct
     2161 tgacgctcag ccgagttcaa catccacacg tggccctctc cgccgcgcgc aaacgcgtca
     2221 gcggtcagtc ccccggcgag agcggcagaa cgtagtgcga agtttccgag tcgctacgca
     2281 gcgaattgca tgtcaggcaa gcccaagttg catgcacaaa ggagctcgat gcaaaagcaa
     2341 gctaatcgta gcaatataga gaaagtcata tgcggccgga tgactatatt cgagcgcgaa
     2401 caaccgagcc cgttaggcca aaccggttgc gacccgtgca acgcgctccg aagccgtcca
     2461 gtttgtcacc ccgcatgcga tcgacccagc cgcgttggac gtgtgaaccc aaacaaattt
     2521 ccgcagcacc tcagcatagt cccaattttg aagaacccgc tttgtctacg gagaagcata
     2581 tgaacgaact gctagattgg ttgagactgc aaaaaggcgg cgtgcggacc tatacccagt
     2641 tttgccagaa ggcgctcgcg ctccgtgctt cgcaacccga gcacgccgcc ctggcgcggc
     2701 ttcttgccga tcttgctgga cgtttcgcgg acgcctacga tggcgagcca ctcttcctcg
     2761 atgttgcgga gcgcgctctc aaccggctga cccggcttgt cgaaagggcc gtgcgagtga
     2821 gctccgctgg cctgccggaa caggtcgccc tcctcaatga gatcggctta gctgagctga
     2881 cataaatgcg ttgagcgtca tgcatgagga gagcgcaatg ctttcgatct ggggcacgag
     2941 aagggggctg gtgcgttgaa ggctcaagga cgaaagcttg atcctgttct ccgaggctcc
     3001 aggcgagacg tttctcttgg tcaggggtag gcgcagagcg cgttcatctc acaggcagcc
     3061 ctgaaggagg accgccatgc cttacctgca ccttgattta cccggcagct atcccgtcga
     3121 ggtaaaacgc gaattggcga cgcggctgtg caagctgtac gcggaagtca tggagacgca
     3181 gctgtggcgg ccgaatgtcg gtatcgccga gctcggcaag cacaatcttt atcacctcgg
     3241 ttccgacggt cttgagtcca ttaccatggt catggtcgag tttcgtcgtg gccgttcggc
     3301 ggaaaggcgg cttgctttag gtagagcaat cgtcgacatc tgtgtcgagc tactcggtgt
     3361 acctcgccgg acagtaatgg tcgagtttac accgcatcgg ggtgaagaga tcctccgcga
     3421 cggaaactgg gtcgcagact ggacacccgc cgaggccgtt acaggctgag gtgcatcatc
     3481 atcctcctcg gcgctgaatt gggaccattc agcgtctgga tttacggcca tgattggctc
     3541 cggagccagt tcgccacggt gcggagccaa ggcgctcgat cagaaaatcg atgaaggccc
     3601 tcaccttggg agcctgatga cggcccggcg gcagcagcgc atacaatggg tagatttccg
     3661 gatcctgcgc gtcctgcagc aagggttgca gcagtcgcgc atggatggag cgcgcgacca
     3721 cgtgctcgct cagccgcagg atgcctcctc ctgcgattgc cagttgcagc aacatctccg
     3781 cgctatcggc gctgacggga cctttgacat cgatccggat cagcttgccg ttcactcgaa
     3841 acggccagct cgccgagccg ggatttcggc tcaaggtcag gcacgcgtga cgaacaaggt
     3901 cggaaggttc gactggccgg gcatgacggg cgaggtagct tggacttgcg caaacgaccc
     3961 gcctgagatc gacgatcttg cgcgagatga gcccagagtc ctcgagcggg cccatccgca
     4021 atgagacgtc tacgttttcg ccgacgagat cgacaacgcg attcgtcacc atgaaatcca
     4081 acgttatgtg aggatagcgc gccaggaagt cgagcaccgc cggcgcaagg tcatgggccg
     4141 caatgaccgg aagcgtatgc acgcgcaaat gcccgcgcgg cgacgcacgc gcggacgcga
     4201 cctcggactc ggccgcctcg atggccccga ggatctcgcg cacgcgatcg aggtagatct
     4261 ctccctcggc ggtcagtgcc aggtgccttg tggtccgatt gatgaggcgc acaccgaggc
     4321 gttgctccag tcgcgtgatg agctttgcca cggcggatgg cgacaagcct acgtcttcgg
     4381 ctgcgcccgc aaagctgccg cgctccgcca cgcgttggaa aatggccatg tcacccgctg
     4441 aactggccat tcgggaaatt ccttcgtgag tgatgggaat tcattgctga ttatcccagc
     4501 tatgtaaatg cataggtttc gtgcctcgcg ccaggcttgc cgccggagtt ggcaaacagc
     4561 gtcaaggcgg gcggtgactt caatgccgag gaagatcagg caatgcctta ccttcagctc
     4621 gacgttccga gccactatcc agtcgacgtg aagcgcggtc tggcccggcg tttgggggaa
     4681 aaatacgcgc gtatcatgca gacaacgccc gagctggtga ccgtggcgtt tcgcgaactg
     4741 ggcgaaggcg gcgtgtggtg ctgcaacgcc ggcgagccgg aaccggcggc cctgctcgcg
     4801 tgtgacatac gtcgcggccg cccgcccgag caacgcgccg aactggcgca ggcattgatc
     4861 gacgcctgtg ttgagacgct agggctgccc agcgaccgcc tcgtcgtgga gtttacgcag
     4921 cacacggctg acgagatgta tcgcgcggga cgcggctggg ccgccgaatg gacgccggcc
     4981 gaggcgacga gtacggtgta aaacctacgg cttgcttgcc caggttagcc tggaggtaac
     5041 catggccatc acgatcaagg tgaacggcgt cgatcggacg gtcgatgtcg acggcgatac
     5101 ccctctcttg tgggtgttgc gcgacgtcct cggtatgacc ggcaccaagt tcggctgcgg
     5161 catggcgctg tgcggcgcct gcaccgtgca catcgacggc gtcgccaccc gttcctgtat
     5221 cacgcctatc gacagcatcg gcgcctccgc ggtcacgacg atcgaggcaa tcggcgcgac
     5281 agcggcgggg gccgcgatcc agaaggcctg gctcgaccgc gaggtcgttc aatgcggcta
     5341 ctgccagtcg ggccagatca tgtccgcctc ggcgctgctc gcgaccaacc cgcatccgac
     5401 ggattccgat atcgacaacg cgatgtccgg caacatctgc cgctgcggga cctatctgcg
     5461 cattcgcgaa gcgatcaagc aggcggcgca atcgagcgga cagggaggct gacaatgcgc
     5521 gtcgatcacc tctcttcttt cgccgacggc tccgctcggt catcatcggc gagcggcgtc
     5581 tcccgacgca ccttgctgca ggccggagcg gccgcgggcg gaggcctgtt gctgagcctg
     5641 cgcctgccgt tcgcaagcgg cgacgccgaa gcggccggcg ccgatggctt cgcgcccaac
     5701 gccttcatcc gcatcggaag cgacgggcag atcgtcctga ccatgcccta cgtcgagatg
     5761 ggccagggca cttatacctc gatcccgatg ctgatcgccg aagagctgga ggtggacctc
     5821 aaacaggtgc ggctggagca tgctccgccc aacgaaaaac tctacggcaa ccccttgatc
     5881 ggaggcatac aggcaaccgg caactcgaac gcggtccgcg ccgcgtggca gccgctgcgc
     5941 caagccggag cgaccgccag aaccatgctc gtctccgcgg cggcgaagcg ctggaatgcg
     6001 gatgcaggat catgccgcgc gcacagcggt gaagtgctgc acccgccgac gggacgacgc
     6061 gccaagtatg gcgaactggc cgccgacgcc gcccgcgtgc cggtgcccaa aaacgtggcg
     6121 ctcaagcggc cgcaggactt caagct
//
LOCUS       DATLJT010000571         1984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_264292,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000571 DATLJT010000000
VERSION     DATLJT010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264292"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..1451
                     /locus_tag="VM910_13225"
     CDS             12..1451
                     /locus_tag="VM910_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVI63531.1"
                     /translation="MEHVWLIAALWIGLALAASVLSVWLRVSVALTEIVIGAVAGNLI
                     HLQLNDWINWLAGFGAILLTFLAGVEIDQAVIKRNFWSTMCIGVMGFFAPYIGVMLFA
                     HFVCAWPWPQAQIAGISLSTTSVAVVYAVMIETGFNRTEIGKIILGACFVNDLGTVLA
                     LGLVFANYNWWLLLFAGVTVAVLIVVPRLVPWFFEKIGTRVSEPEIKLVFFFLLGVGG
                     LAAIAKSEAVLPAYLIGMVLAPTFVKYHVLAHRMRAIAFAAMTPFYFLKAGSLVEVRT
                     VLTAAGLIGGFLAMKMLTKFVGILPLTKAFRFDPREGMYTTLLMCTGLTFGSISALFG
                     LTNHIIDQNQYTILVTAVILSAVVPTLIAERWFQPRFPLESRPNRANEDDYGAKQCTA
                     NREWRGAAAPRRHHLHRLSYIVSLFGDMTYEGARGIVGPYLGLLGVTATPIGIISAVV
                     LASALLQAAALPIFVWLVAREARPRSRLA"
     gene            1463..1882
                     /locus_tag="VM910_13230"
     CDS             1463..1882
                     /locus_tag="VM910_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI63532.1"
                     /translation="MFRMILVANDGSPGGQAAFSAAIDLALKCGGELHMICIEEIPRF
                     AATIDEIEEEKEEAGLRFAAVIEHAKELAGRKGVSLEGHLLAGHPVPTIAEFLRGGTF
                     DLLVVGFMGHSAIYNRLIGSTTDRLVDHAPCAVLVIK"
BASE COUNT          321 a          606 c          623 g          434 t
ORIGIN      
        1 agaggtgagc cgtggaacat gtctggctga tcgccgcgct gtggatcggg ctcgcactgg
       61 cggcgtcggt gctgtcggtc tggctgaggg tctcggtggc gttgaccgag atcgtgatcg
      121 gcgccgtcgc cggcaatctc atccatctgc agctcaacga ctggatcaac tggctggccg
      181 ggttcggcgc catcctgctc acgtttctcg ccggggtcga gatcgaccag gccgtcatca
      241 agcgcaattt ctggtcgacg atgtgtatcg gtgtgatggg gttctttgcc ccctacatcg
      301 gcgtcatgct gttcgcgcat ttcgtctgcg cctggccgtg gccgcaggcg cagatcgccg
      361 gcatttcgct gtcaaccact tcggtcgcgg tcgtctatgc ggtcatgatc gagaccggct
      421 tcaacaggac cgagatcggc aagatcatcc tcggcgcctg tttcgtgaac gacctcggaa
      481 ccgtgctcgc gctcggcctg gtcttcgcca actacaattg gtggctgctg ctgttcgccg
      541 gcgtcacggt tgccgtgttg atcgtcgtac cgcgcctcgt accgtggttc ttcgagaaga
      601 tcggcacgcg ggtcagcgag cccgagatca agctcgtctt tttctttttg ctcggggtcg
      661 gcgggcttgc ggcgatcgcg aaaagcgaag cggtgttacc ggcctatttg atcggcatgg
      721 ttctcgcgcc gaccttcgtc aaatatcacg tgctggcgca caggatgcgt gcgattgcct
      781 tcgccgcgat gaccccgttc tactttctga aagcaggctc gctcgtggaa gtacgaacag
      841 ttctcacggc agcgggactg atcggcgggt tcctcgccat gaaaatgctg accaaattcg
      901 tcggcattct gccgctgacc aaagccttcc gttttgatcc gcgcgagggg atgtacacga
      961 cgctcctgat gtgcaccggg ctaaccttcg gcagcatctc ggccctgttc ggactgacca
     1021 accacatcat cgatcagaac cagtacacga tcctcgtgac cgccgtgatc ctcagcgccg
     1081 tcgtgcccac cctgatcgcc gagcgctggt ttcagccccg cttcccactg gaaagcaggc
     1141 ccaaccgggc caatgaagat gactatggcg caaagcaatg cacggcaaat cgggaatggc
     1201 gaggtgctgc cgcgccgcgc cgccatcatc ttcatcgtct ttcttacatc gtcagcctgt
     1261 tcggcgacat gacctatgag ggcgcgcgcg gcatcgtcgg gccttatctc gggctgctcg
     1321 gcgtcacggc gacgccgatc ggtatcatct ccgccgtcgt cctcgcctcg gcattgctgc
     1381 aggccgcagc gttgccgatt ttcgtttggc tcgtcgcgcg cgaggcgcgg ccgcgaagcc
     1441 ggttggcctg aaggagaagc caatgtttcg gatgatcctt gttgcgaatg acggatcgcc
     1501 cggcgggcag gcggcctttt ccgccgcgat cgatttggcg ctcaagtgcg gcggcgaact
     1561 gcacatgatc tgtattgagg agatcccgcg ctttgccgcg acgatcgacg agatcgaaga
     1621 ggaaaaagag gaagctggcc tccgcttcgc ggcggtcatc gagcatgcca aggagcttgc
     1681 cggccgaaag ggtgtatcgc tcgaaggcca tctgctggcc gggcatccgg ttccgaccat
     1741 cgctgagttt ctgcgcggcg gcaccttcga tctcttggtg gtcggcttca tggggcattc
     1801 ggccatttac aaccggctca tcggcagcac caccgaccgg ctggtggatc acgcgccttg
     1861 cgccgttctc gtcatcaagt gatgtgtacg cgccgcgttg ttggcgttgc agacggctgc
     1921 tacggggtcg actgcggtcg aattcaccgt tccatgcgaa tggctcggaa gggcggcgag
     1981 cggt
//
LOCUS       DATLJT010000572         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_264338,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000572 DATLJT010000000
VERSION     DATLJT010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264338"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..522)
                     /locus_tag="VM910_13235"
     CDS             complement(103..522)
                     /locus_tag="VM910_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI63533.1"
                     /translation="MFRKILVANDGSPGGQAAFSAAIDLALKCGGELHMICIEEIPRF
                     AATVDEIEEEKEEAGLRFAAVIEHAKELAGRKGVSLEAHLLAGHPVPTIVEFLRGGTF
                     DLLVVGFMGHSAISNRLIGSTTDRLVDHAPCAVLVIK"
     gene            complement(534..1733)
                     /locus_tag="VM910_13240"
     CDS             complement(534..1733)
                     /locus_tag="VM910_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI63534.1"
                     /translation="MHAKSGMARCCRAAIIFIVFLGIVSLFGDMTYEGARSIVGPYLG
                     LLGVTATTIGIVSGFGELMGYGARLLSGYLGDRTGSYWAVTIIGYVLNLFAVPLLALA
                     GAWQIAVVLIVLERTGRGIRSPVRDAMLSHAASQTGLGWGFGLHQALDQLGAVVGPLI
                     DSGILFAGNRYQTAFAWLLVPALLSMTVLLAARLLFPRPHDFELAPPLADTGYLAPVF
                     WVYIAAVACIAAGYADFPLIAYHFDKAQVMPTAWIPILFAVAMGVDGVGALALGSLFD
                     RIGIRTMVLATIVSAAAAPLVFLGNFPLAVIGMVCWGLGTGAQDSVMRATMVRLAPQQ
                     RRATAFGIMNAIYGVAWFAGSVLLGVLYDRSISAVVLASALLQAAALPIFVWLVAREA
                     RPRSRLA"
     gene            complement(1755..2087)
                     /locus_tag="VM910_13245"
                     /pseudo
     CDS             complement(1755..2087)
                     /locus_tag="VM910_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
BASE COUNT          469 a          703 c          721 g          327 t
ORIGIN      
        1 accgctcgcc gcccttccga gccattcgca tggaacggtg aattcgaccg cagtcgaccc
       61 cgtagcagcc gtctgcaacg ccaacaacgc ggcgcgtaca catcacttga tgacgagaac
      121 ggcgcaaggc gcgtgatcca caagccggtc ggtggtgctg ccgatgagcc ggttggaaat
      181 ggccgaatgc cccatgaagc cgaccaccaa gagatcgaag gtgccgccgc gcagaaactc
      241 aacgatggtc ggaaccggat gcccggccag cagatgggct tcgagcgata cacccttccg
      301 gccggcaagc tccttggcat gctcgatgac cgccgcgaag cggaggccag cttcctcttt
      361 ttcctcttcg atctcgtcga ccgtcgctgc aaagcgcggg atctcctcaa tgcagatcat
      421 gtgcagttcg ccgccgcact tgagcgccaa atcgatcgcg gcggaaaagg ccgcctgccc
      481 gccgggcgat ccgtcattcg caacaaggat cttccgaaac attggcttct ccttcaggcc
      541 aaccggcttc gcggccgcgc ctcgcgcgcg acgagccaaa cgaagatcgg caacgctgcg
      601 gcctgcaaca atgccgaggc gaggacgacg gcggagatcg agcggtcata caacaccccc
      661 agcaggacgc tgccggcgaa ccaggcgacg ccatagatcg cattcataat gccgaaggca
      721 gtggcacggc gttgctgcgg cgcgaggcgc accatcgttg cgcgcatgac cgaatcctgc
      781 gcgccggtgc cgaggcccca gcacaccatg ccgatgaccg cgagcggaaa gttgccgagg
      841 aagacgagcg gcgcggcggc ggccgacacg attgtcgcca gcaccatcgt gcggatgccg
      901 atccggtcga acagcgagcc caaggcaagc gcgccgacac cgtcgacgcc catcgcgacc
      961 gcaaacagga tcgggatcca cgccgtcggc atgacctgcg ccttatcgaa atggtaggcg
     1021 atcagaggaa agtcggcata gccggccgcg atacaggcaa ccgcggcgat gtagacccaa
     1081 aaaaccggcg cgaggtatcc ggtgtcggca aggggcggcg ccagctcgaa atcgtgcggc
     1141 cgcggaaaca gcaaccgcgc ggcgagcagg acggtcatgg acaagagcgc gggaaccagg
     1201 agccaggcga aggcagtttg gtagcggttc ccggcgaaca ggatcccgct gtcgatgagc
     1261 ggcccgacaa cggcgccgag ctgatcgagc gcctggtgca gcccgaagcc ccagccgagc
     1321 ccggtctggc tcgcggcgtg cgacagcatc gcatcgcgca ccggcgagcg gatgccgcgg
     1381 ccggtccgct cgaggacgat cagcacgacc gcgatctgcc aggcaccggc gagcgccaag
     1441 agcggcacgg cgaacaggtt cagcacatag ccgatgatcg tgacggccca atagctgccg
     1501 gttcggtcgc cgagatagcc ggagagcagg cgggcgccat agcccatcaa ttcgccgaag
     1561 ccggaaacga tgccgatcgt cgtcgccgtg acgccgagca gcccgagata aggcccgacg
     1621 atgctgcgcg cgccctcata ggtcatgtcg ccgaacaggc tgacgatgcc aagaaagacg
     1681 atgaagatga tggcggcgcg gcagcacctc gccattccgg atttcgcgtg cattgctttg
     1741 cgccatagtc atcttcattg ccccggttgg gcctgctctt ccagtggacg gaattggggc
     1801 tgaaaccagc gctcggcgat cagggtgggc acgacggcgc tgaggatcac ggcggtcacg
     1861 aggatcgtgt actggttctg atcgatgatg tggttggtca gtccgaacag ggccgagata
     1921 ctgccgaagg tcagcccggt gcacatcagc agggtcgtgt acatcccctc gcgcggatcg
     1981 aaacggaaga ctttcgtcag cggcagtatg ccgacgaatt tggtcagcat tttcatgccg
     2041 aggtacgcgc cgatcatccc catcgccgtc agaactgttc gtacttcgac gagcgagccg
     2101 gtgaagtcct aagcttgctg ggaaaagggt tggggtgcac cctcgcctga ttggcaatca
     2161 acgaggaccg gtattgggcc ggcctctgcg aaaggagcac cccgatggag agaataccgg
//
LOCUS       DATLJT010000573         7436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2101779,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000573 DATLJT010000000
VERSION     DATLJT010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101779"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VM910_13250"
     CDS             <1..383
                     /locus_tag="VM910_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI63535.1"
                     /translation="DLITQMRKVFGEEGLDRLVALRTVEQERAYGARLAARRTLRTRL
                     ETLARVRSDEGYMAELRREPETGAWLLVEHHCPICAAARLCTGLCREELALFQRVLGS
                     GVAVERVTHILAGAGRCAYRVTPL"
     gene            complement(703..1434)
                     /locus_tag="VM910_13255"
     CDS             complement(703..1434)
                     /locus_tag="VM910_13255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI63536.1"
                     /translation="MDFDLIASEYARHRKIMLPVLDGLRDFVAAIGHKAAVVELGSGT
                     GNYIIALAESTGCMAWGIDCAKGMLAQAVSRGSEVQFAIGDATKLALAADRFDLVYSV
                     DVIHHIGPTDRLVSYYQEARRVLKEGGRVCTVTHSEALLRSSAVLSRYFPEAVELIVA
                     DYPRIDALRDVMATVGFADIVERIVGHTVRVTNAAAYAAKAYSALNFISGKAFERGLR
                     RLEADLATGPIHGTRRALMLWGTKR"
     gene            complement(1504..2238)
                     /locus_tag="VM910_13260"
     CDS             complement(1504..2238)
                     /locus_tag="VM910_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63537.1"
                     /translation="MRLQFLGCGDAFGSGGRFNTCFHVTTGSTQFLVDCGASSMIAIR
                     RFGVEPNAIETIFITHLHGDHFGGLPFFILDAQLVSRRTTPLTVAGPPGLRDRLREAM
                     EVLFPGSAEVARRFQVCVIELDAGAASMVNGVTVTPFLMKHACGAPPFALRLECDGKS
                     LAYTGDTEWVDALISAAREADLFIAEAYFWERKVKYHLDWVTLKSHLGEIRAKRVILT
                     HMSGDMLSRPIEEGGGVASDGMVIEF"
     gene            complement(2138..2365)
                     /locus_tag="VM910_13265"
     CDS             complement(2138..2365)
                     /locus_tag="VM910_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63538.1"
                     /translation="MDESFEAMVARIGARETPLIATLQAIPRYQGSHLDLRSGQYSNA
                     TAIPRLWRRLWQRRPVQHMLSRNHWIDPIPG"
     gene            complement(2940..3290)
                     /locus_tag="VM910_13270"
     CDS             complement(2940..3290)
                     /locus_tag="VM910_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63539.1"
                     /translation="MRDLALQAAGVLAILVAVAHSVTAELRLFANARIEPQRVRRVLR
                     MVCQASAVDWIGIGLLLIAAPSLGSQVARQWVIAVAVVVYGYAAVGNAVATRGRHVGW
                     CLMSGVIALALVGL"
     gene            3374..4054
                     /locus_tag="VM910_13275"
     CDS             3374..4054
                     /locus_tag="VM910_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI63540.1"
                     /translation="MTKRLTAQDWIDFALTTFAHEGFDALKADVLARKLGVSRGSFYW
                     HFTDLGAFHARVIEQWKQVATEAIIADVERYQSGEERLDALLRHAFGHGAALEIRMRA
                     WADNNAEAARAVSDIDRRRQEYIEQLLVGAGIVLPLAATRTLLLYWTYLGAALSRSRL
                     SGERLNRIVAELKRIGLGAFPGTHRSANHGSRSRSSARDEPALADQARPSRRIGGSRD
                     RTTARKRS"
     gene            complement(4200..4340)
                     /locus_tag="VM910_13280"
     CDS             complement(4200..4340)
                     /locus_tag="VM910_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63541.1"
                     /translation="MERFLADVDTHAGNSQIRAAPFYQKTLWTHEFSPGAGLVSRTLV
                     AV"
     gene            4388..5053
                     /locus_tag="VM910_13285"
     CDS             4388..5053
                     /locus_tag="VM910_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63542.1"
                     /translation="MGDAVKLESDIMMRYVAQAVENWNRLASSDTTTVANPQDPLLEA
                     NAPLVDLVEAVRALPYGRPSDRSVDGLLRERRGTCSTKHLFLAQALRERFPHTQPKIV
                     HRVYRAEREQIRERYGDEVAAVVPEDGLVDVHRYLLIRVGGRQITLDVTFPGGEPWDG
                     RSSLPLACGPGEDIAAGDNPDWDKRALEARYCTPAAREPFIAALTAASEARRRRSPSG
                     HAS"
     gene            complement(5213..5392)
                     /locus_tag="VM910_13290"
                     /pseudo
     CDS             complement(5213..5392)
                     /locus_tag="VM910_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
     gene            complement(5753..6574)
                     /locus_tag="VM910_13295"
     CDS             complement(5753..6574)
                     /locus_tag="VM910_13295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI63543.1"
                     /translation="MGLRPEAPAELHGAVSAIRGEREETAMRLADKVAIISGAASGMG
                     AATARMFAREGAKVVIADVLEQEGRQIADAIGAAARFEPLDVTKEEDWAAVVAATTRH
                     FGKLDVLVNNAGISGSAERDFYSTEAWHRIMAVNATGVFFGIKYAIPAMVANGGGSIV
                     SLSSIAGIIGSEHVHMAYNASKAAVRLMTKSVAVQHAKDKIRANSVHPGIMPPMRTSG
                     RTADPAVRAERMRVIPMRRPGEVDEVAYAILFLASDESSYITGSEIHVDGGAIAI"
     gene            complement(6529..7329)
                     /locus_tag="VM910_13300"
     CDS             complement(6529..7329)
                     /locus_tag="VM910_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI63544.1"
                     /translation="MAYDYSQYGGVLVGRQDKVLTITLNAPETLNAFTGAMHTSMSRI
                     WDDVNDDPDVHVVVLTGAGRAFSAGGNVIAMQKKIDDPDAWEAGMPEARRIVFRMLEC
                     DKPIIARVNGHAVGLGATIALLCDIIIAADHAKIGDPHVNAGLVAGDGGALIWPQLIG
                     FARAKEYLFTGDLMTAAEAERIGLINHVVPADQLDDKVYGLARRLAGGAMRSIRWTKQ
                     AVNIPLRQLAHSMMDLSLSSETHSNLTKDHQEAVWAFAQKRRPNFTGR"
BASE COUNT         1382 a         2400 c         2311 g         1343 t
ORIGIN      
        1 cggacctgat cacgcagatg cgcaaggtgt tcggcgaaga agggctcgat cggctggtgg
       61 cgctcaggac cgtggaacag gaacgggctt acggcgctcg actcgccgcc aggcggacgc
      121 tgcggacgcg gctcgagacg ctggcccggg tgcgcagcga tgagggctac atggccgaac
      181 tccggcggga gcctgaaacc ggcgcctggc tgctcgtcga acaccattgc ccgatctgcg
      241 cggcggcgcg gctgtgcacc ggcctctgcc gagaagagct ggcgctgttt cagcgcgtgc
      301 tcgggagtgg ggtggctgtt gagcgtgtca cgcacatcct ggcgggcgcc ggccgctgcg
      361 cttaccgcgt gaccccgctg tagctaacgg ctacatgccg catcaggcac gcatctcccg
      421 ttctcgggaa tgtagtgcca gttgcctcgc tcatcggacg cgaaggaaag taaaataaat
      481 atcaaacgcc ggtttatatg ctcaccctcc cgccgcgcgc atcccgcttg gcgggtccct
      541 ccttcttcca gcaaacgggg agagggctgg gggtgagggg tcccgcgcgc tacgtcggtg
      601 gccagctttt cacccggcgt ggtgcgagcc ttctgctcgt tggctgtctc cctgagttct
      661 gccgtgcgga ggcgcgccgc ttcaccgctt cgtgccccac actcaccgct tcgtgcccca
      721 cagcatcagc gcccgccggg tgccgtgaat cggtccggtg gcgagatccg cctcgagacg
      781 gcgcagaccc cgctcgaacg ctttgccgga tatgaaattc agcgcggaat acgctttcgc
      841 cgcataggcc gcggcattgg tcaccctcac cgtgtggccg acgattcttt ccacgatatc
      901 ggcaaatccg acggtcgcca taacgtcgcg aagcgcatcg atacgcggat agtccgctac
      961 gatcagctcg acagcctcgg ggaaatagcg gctcagcacc gcgctcgagc gcaagagagc
     1021 ctcggaatga gtgaccgtgc aaacccggcc gccttccttc aagacacgcc gagcctcctg
     1081 gtagtagctg accaacctgt cggtcgggcc gatgtggtgg atcacgtcga ccgagtagac
     1141 cagatcgaac cggtcagccg ccaaggccag cttcgtcgca tcaccgattg cgaactgcac
     1201 ttcgctgccg cgactcactg cctgggctag catccctttg gcgcaatcga tgccccaggc
     1261 catacagccc gtggactcgg cgagcgcaat gatgtagttg ccggtgccgc tgccaagctc
     1321 cactactgcg gccttgtggc caatagcggc gacgaaatcg cgtagtccgt cgagcaccgg
     1381 cagcatgatc ttccggtgcc tcgcatattc ggatgcaatc agatcgaagt ccatgagcgt
     1441 gctcgcggtg atgttggacg ggacgcgacc aatgggaagg aatggccgtc acagccggca
     1501 aactcagaac tcgatgacca ttccgtcact ggcgacgcca ccgccctcct cgatggggcg
     1561 gctaagcata tccccgctca tatgcgtcag gatcactcgc ttcgcacgga tctcgccgag
     1621 atgactcttc agggtcaccc aatccaggtg gtatttgacc tttcgctccc agaaatacgc
     1681 ttcggcgatg aagaggtcgg cctcgcgcgc cgccgatata agcgcgtcca cccattctgt
     1741 atcgccggtg taggcgagcg atttgccgtc acattccagg cggagcgcga acggcggagc
     1801 gccgcaagcg tgcttcatca gaaatggtgt gacagtgaca ccattgacca tactcgctgc
     1861 cccagcgtcg agctcgatca cgcagacctg aaagcgccgc gcgacctccg cagagcccgg
     1921 aaacaacacc tccattgcct cgcgcagccg gtcccgcaag cccggcggcc cggccaccgt
     1981 cagcggggtc gtgcggcggc tgaccaattg tgcgtccaga ataaagaacg gcaagccgcc
     2041 gaaatggtcg ccgtgcaaat gcgttatgaa gatggtttcg atcgcattcg gctcgacgcc
     2101 gaagcgccga atggcaatca tcgacgaggc gccgcagtca accaggaatt gggtcgatcc
     2161 agtggttacg tgaaagcatg tgttgaaccg gccgccgctg ccaaaggcgt cgccacaacc
     2221 gaggaattgc agtcgcattg gagtactgcc ccgatctaag gtccaggtgg cttccctgat
     2281 agcgcggaat cgcctgcagc gtcgcgatga gcggtgtctc gcgcgctccg atgcgcgcga
     2341 ccatcgcttc gaaactctcg tccaagccgt attcctcggc atacaacgcg ccatggcgat
     2401 ggattatcca acccatgtcg cccgccgatg ggcacgaagc atgtaggagg gctcaccggg
     2461 cttgcgtcga tcgccgagca atctctcaac cgtctcgagc gcagcaacca gccgcgcttg
     2521 ctcgggaccg ctcagctgac ccaacattgt cgcgatctca tcgtgggaac ggtcgtcgct
     2581 tgagggctcc actcgcgccg cgcggtttcg tgtcggtcag ctcggcgacc agaacctttc
     2641 ttttgtctct gggctcagga ggaccacgac gaggaatatc gcaaggccga ccacgtggtg
     2701 atgacgatgg gagccgccag ggttatttga tgggtggtgg cgtacaaaac cagtagcggc
     2761 gagatcaggc cgcccctcga acacgatgac gggacccggg ccacgggtgt cttcgtactc
     2821 gatggcccct gccgcaacct cgaggtgcgc catgacagtc gcctcaaaag ggcgggttat
     2881 tcttttgcct caggacgtcg cgccttcata cgtcgtgctc gggcggttct tgtcccgggc
     2941 tacaggccca cgagagccag cgcgataacc ccgctcatca agcaccatcc aacgtgcctc
     3001 ccgcgcgtgg ccacagcgtt gccgacggcg gcgtatccat acacgacaac agccaccgca
     3061 atgacccatt ggcgggcgac ctgcgagccg agcgacggcg cggcgatcag caagagccca
     3121 atgccgatcc agtcgacggc gctggcttgg cacaccatcc gcagcactct ccgcacccgt
     3181 tgcggctcga tgcgcgcgtt ggcgaatagt cgcaactcgg cagtgacgct gtgtgcgacc
     3241 gcgacaagaa tggccaacac acccgcggcc tgaagcgcca gatctcgcat tcgccgcctc
     3301 catacagaag tgcatggtgg gaactatacc ttacccgttg ccgaagtcaa tacactactg
     3361 tacggtgtgc ctcatgacca agcggctgac ggcacaggac tggattgatt tcgccctgac
     3421 gacgttcgcg catgagggct tcgatgctct gaaggcggat gtgcttgccc gaaagctcgg
     3481 cgtctcgcgc ggcagcttct attggcactt caccgacctt ggcgcttttc acgctcgggt
     3541 gatcgagcag tggaaacagg tggccacgga agcgatcatc gctgacgtcg agcggtatca
     3601 gtcaggcgaa gagcgcctcg atgcgctctt gcgccatgcg tttgggcacg gcgccgcact
     3661 ggaaatccgc atgcgtgcct gggccgacaa caacgccgag gcagcgcgag ccgtgagcga
     3721 catcgatcgc agacgacaag agtacatcga gcagttgctc gtaggagcgg gcattgtgct
     3781 gccgttggcg gcaacgcgca cgctactcct gtattggacc tacctcggtg ctgcattgag
     3841 ccgcagccgg ctcagcggcg agcggctcaa tcggattgtg gccgaactca agcggatcgg
     3901 actgggtgcg ttccctggga cccaccggag tgcaaatcac ggctcccgct ctcgttccag
     3961 cgcgcgcgac gagccggcgt tggcggatca ggccaggccg tcgcgccgca tcggcggcag
     4021 tcgcgatcgg actaccgcaa gaaagagatc ctgaggacca gcgcgacact agggggatat
     4081 ccgcgcgcga gcgcaggccg gcttggcagt atgtgccatc atttcaggag caaagagcag
     4141 ttggctgcgg cgctccatgt gaggacctat gcgactatca ggagagcgtt ctcgccgagc
     4201 tacaccgcca ccagcgtgcg cgagaccagg ccagcgccag gcgagaattc gtgcgtccac
     4261 aatgtcttct gatagaatgg tgcggctcga atttgactgt tgccagcatg ggtatcgaca
     4321 tcggcaagaa agcgttccat ctcgtcggcc tcgaccggcg tggcgcgatc ttgctgtcga
     4381 gcgggcagtg ggcgacgcgg tcaagctgga gtccgacatc atgatgcgct acgtcgcgca
     4441 ggcggtcgaa aattggaacc ggctcgcgag ttccgacacc accacggtcg cgaatccgca
     4501 agatccgctg ctagaagcaa acgcgccatt ggtcgacctc gtcgaggccg tgcgagcgtt
     4561 gccatacggc cgcccgagtg accgctccgt cgatggcctg ctccgagagc gccgcggcac
     4621 gtgttcgacc aaacacctgt tcctcgcaca ggcactacgc gagcgcttcc cgcatacgca
     4681 gccgaagatc gtgcaccgcg tctaccgcgc cgagcgcgag cagatccgcg agcgttacgg
     4741 agacgaggtc gcagccgtcg tcccggagga cggtctggtt gatgtccatc ggtatctgct
     4801 gatccgggtc gggggaaggc agatcacgct cgacgtgaca ttcccgggtg gcgagccctg
     4861 ggacggtagg tcgtcccttc cgttggcctg cgggccaggt gaggatatag ctgcaggcga
     4921 caaccccgat tgggacaagc gagcgctgga agcgcgctac tgtacccccg ccgcgcgaga
     4981 gcccttcatc gcggcactca cagccgcttc ggaggcgcgc cgccgccgat cgccatcagg
     5041 ccatgcgtcg tgatcatcgc taccatcagg acctcctcct cacccgtaga cgtcgaatcg
     5101 ctccagatac gacagatccg ggcataattc ggcaaagccg cagtgcagat ggaggcgtcg
     5161 gcggttgcga tttagtgagg cgggccatca gccccggcca gtgaaatcca ccacgtctaa
     5221 cgatctcgat gaaattggga ctgcgcatct cggccggcgc ctcatcggcg cggtaatcac
     5281 tgccagcttc gaggagcaga acggaggtcg acggattttc ggaaaggcgg gctgcaagca
     5341 ccgccccagc ggatcctcct ccaaccacga tgatgtcgta gctgtctatc atcggatcca
     5401 tgctgctacg ctatattctg cggggctcaa tcgccaatat agcagattcg tggcggaggt
     5461 cagtatgtgc gcgtcagcgg accccttcta caaatgctcg tctgggattc gtcgatgtca
     5521 aagcggcccg cctgtcgctg atgtccggaa cgggtcacgg gcagcggtgc cgtcaaccgt
     5581 cgcgcaccgc cggtgtactt tcaactacgg aagagccctc accctgccga aggccgctgt
     5641 gatagcttgt cctgtgcggg cgcaattccg cgagcccagc gatcctgctc ggccggaggg
     5701 gtgtcgcggc cgccaattag tcacgagcaa tgacgccgtg ccgggcgcag gcctatatgg
     5761 cgatagcccc gccgtccacg tggatttccg aacctgtgat gtaggacgac tcatcggaag
     5821 ccaggaacag aatggcgtac gccacctcgt caacttctcc cggccggcgc atcggaatga
     5881 cgcgcatgcg ttcggcccgc acggcgggat cggcggtacg accggacgtg cgcatcggcg
     5941 gcatgatgcc gggatgaacc gagttggcgc gaatcttgtc cttggcgtgc tgcaccgcga
     6001 ccgatttggt catcagacgg accgccgcct tcgaggcgtt gtaggccatg tgaacgtgct
     6061 cgctgccgat gatgccggca atcgacgaca gactgacgat cgacccgccg ccgttggcaa
     6121 ccatggccgg gatcgcgtac ttgatgccga agaacacgcc ggtcgcgttg accgccatga
     6181 tccggtgcca ggcttcggtg ctgtaaaaat cccgctcggc gctgccggag atgccggcgt
     6241 tgttgaccag cacatcgagc ttgccgaaat gccgggtggt ggcggccacg acggcggccc
     6301 aatcctcctc tttggtgaca tcaagcggtt cgaaccgggc cgcggcaccg atagcatcgg
     6361 caatctggcg gccttcctgc tccaacacgt cggcgatcac cactttggcg ccttcgcgcg
     6421 cgaacatgcg cgccgtggcc gctcccatgc cggatgccgc gccggagatg atggcgacct
     6481 tgtccgctag tctcattgct gtctcctccc tttcgcctcg aatggcgctc accgccccgt
     6541 gaagttcggc cggcgcttct gggcgaaggc ccataccgct tcctggtggt ccttggtgag
     6601 attcgaatgc gtctcggaac tgaggctcag gtccatcatc gaatgggcaa gctgacgcag
     6661 cgggatgttg acggcctgct tggtccagcg gatcgaccgc atcgcgccgc cggccagtcg
     6721 tcgggccagc ccataaacct tgtcatcgag ctgatcggcc ggcacgacat ggttgatcag
     6781 cccgatccgc tcggcctccg ccgcggtcat caggtcgccg gtgaacaagt actccttggc
     6841 gcgtgcgaag ccgatgagct gcggccagat caatgcgccg ccgtcacccg ccacaaggcc
     6901 ggcgttgaca tgcggatcac cgatcttggc atggtcggcc gcgatgatga tgtcgcacag
     6961 cagcgcgatc gtcgcgccga ggccgacggc atggccattg acccgcgcga tgatcggctt
     7021 gtcgcattcc agcatgcgga acacgatccg cctcgcctcg ggcatgccgg cttcccatgc
     7081 gtcgggatcg tcgatctttt tctgcatggc gatcacgttc ccgccggccg aaaacgctcg
     7141 gcctgcccca gtcaggacga cgacatgcac gtcgggatcg tcgttcacat cgtcccagat
     7201 gcgggacatc gaggtgtgca tcgccccggt gaacgcgttg agggtctcgg gcgcgttcaa
     7261 ggttatcgtg agtaccttgt cctgccgccc tacaagcacg cccccatact gggaataatc
     7321 gtaggccatc gtttcctccc gtgatcgttg cccgaaccgc cgcagtctac gcgtttcggc
     7381 gacacttcga caaaagcgat gacttggcca tccgatcgca cagccgagaa cttcgc
//
LOCUS       DATLJT010000574         4773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_952993,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000574 DATLJT010000000
VERSION     DATLJT010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952993"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VM910_13305"
     CDS             <1..385
                     /locus_tag="VM910_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HVI63545.1"
                     /translation="PRTVAELAGHNCLGYTLAQSGADRWLMGKRAEISVAVSGNLRAN
                     NGDALLAAAIAGQGIVYQPTFIAADDLRAGHLVALALDQPANESLAVHAVYLPDRHPP
                     AKVRAFIDFLASQFAPEPPWDRGIP"
     gene            462..2150
                     /locus_tag="VM910_13310"
     CDS             462..2150
                     /locus_tag="VM910_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVI63546.1"
                     /translation="MEKQQAAQPPTDKAWPLFRSLDGYRVGYLPNDLIAGLTLAAIAI
                     PEQMATARLGGFAPQIGFFAFIAGSIGFAMLGGNRFLSCGADSTITPIFAGGLAAVAA
                     AGSSDYQALAIALALMVGVMTIAAGLFRLGGIANLLSVPVTVGFLAGISVHILVSQMP
                     GVLGLPSPRGPTLQRIAELAAHVGDANIFTVCIGFGVLAVVAICEAVNARIPGALIGL
                     VAATLAVIAADLERKGVAVVGTVPAALPKPTLPDLAPERWVRLIPLGLLVTVVVMVQT
                     AATTRAFLSDPDRPADVDRDFLGVGVGSLLAGLFGAFAVDASPPRTGIVSETGGKSQL
                     SGLFAAAILLALIAFGATLLKHVPDAALGGVLLFVALRIIRFRQIAMIWRQSMAEFLL
                     IVATAAAIIMLPIEQGVGIGIILSLLHGIWSTTRARLVRFERVPGTTIWWPTNPHVKG
                     ERLPNVTVVGLQAPLSFLNASGFQSDALDVVKSEKPKLLVLEASGMPEIDFTAAQNLI
                     DLIRECGSRGITVAVARLESLRAQEAFERFRLYDVLPRDRVFHSVAEAVAALAA"
     gene            complement(2175..2570)
                     /locus_tag="VM910_13315"
     CDS             complement(2175..2570)
                     /locus_tag="VM910_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63547.1"
                     /translation="MWWFSRKKPKEAWEETIQWPIGDIEAAERIRHVCRSAADSAASV
                     GHPADSRKKRDAKKNLDDGERYKGAARAAMEIAMKMSDDLMRDAGVGEIIELCLKAND
                     IKTARILFRAIQSPSIRDGMLSDHPALRP"
     gene            complement(2665..3906)
                     /locus_tag="VM910_13320"
     CDS             complement(2665..3906)
                     /locus_tag="VM910_13320"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI63548.1"
                     /translation="MSIFRIGVAVLVAVTALASAEPASAGLRIRLGGPFGVIRSAFAH
                     MLPLRGLHHRHYARRGGAVRLASVRDTVDTTSRTDGFRNAEWSANPSARVQLAASAAL
                     AGWHGGRGAHGWWRHEDGGYGWVGPLFWPFAYQDIYDYTVWGDTSGFWGYGYGDLYAA
                     VFAPYGNDDLTRYVAPSRGRRHGRIISVASMCGDAKDDAATAAVDQIRQVVQLNEEQS
                     AALDDLAGASVEAAKIVRAVCPGEVAATASLRLAAMQQRLEAMVSAVARVRPALERFY
                     ELLDDEQKGKLEAFGEGQRKQRAAPKPSPVRSCQDAQPASFQWPGGEIEARLHLDDAQ
                     RAELAVLQHMSAKARDILSYDCQPDEGILPPDRLASVDTRLDALLDAIKLVRPALDDF
                     YATLSDEQKAQFEAIGPKRTS"
     gene            complement(3945..>4773)
                     /locus_tag="VM910_13325"
     CDS             complement(3945..>4773)
                     /locus_tag="VM910_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI63549.1"
                     /translation="AVWGDGFGFWGYGYPDIYAGIFAPYGYGELSGYMMPPSGGRRYR
                     GAVSLARMCSDDSRDIAGLPVDQIGQAIEPTEAQRPALDDLADASIKAAQMIRASCPT
                     QVAATAPARLAAMEQRVEAMISAVGLVRPPLERLYALLNDEQKVRLNALAEDQRGRSP
                     DNANGPAAQGCSAAQPAGLAWPAAEIEARLHPNDIQRAALQVLQDTTARAADALKAAC
                     QPAEAITPVARLDAVGKRLDAMLQAVKSVRAALEDFYATLSDEQKAQFEAIGPRRTS"
BASE COUNT          726 a         1643 c         1533 g          871 t
ORIGIN      
        1 gccgcgcacg gtcgcggaac tcgccggtca caattgcctc ggctacacgc ttgcgcaatc
       61 cggcgccgac cgctggctga tgggcaagcg ggccgagatc agcgttgccg tttcgggaaa
      121 cctgcgcgcg aacaacggcg atgcgctgct ggctgccgcc atcgccgggc aagggatcgt
      181 ttatcaaccg accttcatcg ccgccgatga tttgcgcgcc ggccatctgg tcgcccttgc
      241 gctggatcag cccgcaaacg agtccctcgc ggttcatgcg gtctatctgc cggaccgtca
      301 tccgccggcc aaggtacgcg ccttcatcga ctttctcgca agccaattcg cgcccgagcc
      361 gccctgggac cgcggcatcc cctgatattg cctcgagcca tcgacgggct tgccgctgcg
      421 gcgccgatcg gatttgctcc acgattcaag ggaggagacg cgtggaaaag caacaggcag
      481 cgcagccgcc aacggacaaa gcctggccgc tgttccgctc actggacggc taccgggtgg
      541 gctacctgcc gaacgacctg atcgcagggt tgacgctcgc ggcgatcgcg attcccgagc
      601 agatggcgac cgcgcggctc ggtgggttcg cgccgcaaat cggcttcttc gccttcatcg
      661 cgggctcgat cggctttgcc atgctgggcg gcaaccgatt cctgtcctgc ggcgccgact
      721 ccacgatcac gccgattttt gctggcgggc ttgcggcggt tgccgccgcc ggctcgtccg
      781 actaccaggc gctcgcaatc gcgctggcgc tcatggtcgg cgtcatgacg atcgccgccg
      841 gcctgttccg cctcggcggc atcgccaatt tgttgtcggt gccggtcacg gtcggctttc
      901 tcgccggcat ctccgtgcac atcctggtgt cacaaatgcc cggcgtgctc ggccttcctt
      961 cgccgcgcgg gccaacgctg caacgcatcg ccgagctcgc cgcgcacgtc ggtgacgcca
     1021 acatcttcac ggtctgtatc ggattcggcg tgctggcggt ggtcgcgatc tgcgaggctg
     1081 tcaacgccag aataccgggc gctctgatcg ggctggtggc ggcgacactg gccgtgatcg
     1141 cggccgacct cgagcgcaag ggcgtggccg tcgtcggcac cgttcccgcc gcgctgccga
     1201 agccgacatt gccggacctt gcgcccgagc gatgggtccg cctgattcct ctcgggcttc
     1261 tcgtcaccgt cgttgtcatg gtccagacgg ccgcgaccac gcgggcgttt ctgtccgatc
     1321 ccgacaggcc tgcggacgtc gaccgcgact ttctcggcgt cggcgtgggc tcgctgcttg
     1381 cgggcctgtt cggcgcattt gccgtcgatg cgagcccgcc acgcaccggc atcgtctcgg
     1441 agaccggcgg caaatcgcaa ctgtcggggc tgtttgcggc ggccattctg ctggcgctga
     1501 tagcattcgg cgcgacgctg ttgaaacatg ttccggacgc cgcgctcggc ggcgtgttgt
     1561 tgttcgtcgc cttgcggatc atccgtttcc ggcagatcgc catgatctgg cgccagtcga
     1621 tggcggagtt cctgctgatc gtcgcgaccg cggcagccat catcatgctg ccgatcgaac
     1681 agggcgtcgg catcggtatc atcctgtcgc tcctgcacgg catctggagc acgacgcgcg
     1741 cccgcctcgt gcgattcgag cgggtgccgg gcacgacgat ctggtggccc accaatccgc
     1801 acgtcaaggg cgagcgcctg ccgaacgtga ccgtggtggg gttgcaggcg ccgctgtcct
     1861 tcctcaatgc gtcgggcttt cagtcggacg cactcgacgt cgtgaaatcc gagaagccga
     1921 aactgctcgt gctggaggcg agcggcatgc ccgagatcga cttcacggcg gcgcagaacc
     1981 tgatcgacct gatcagggaa tgcggcagcc ggggcatcac cgttgccgtg gcccggcttg
     2041 aatcgttgcg ggcacaggaa gccttcgagc ggttccgact ttacgatgtg ctgccgcggg
     2101 accgcgtgtt ccacagcgtt gccgaggcag tcgctgcgct cgcggcgtaa tgagtccgca
     2161 tcgggcgtcg tggctcacgg cctcagcgcg ggatgatcgc tcagcatgcc gtccctgatc
     2221 gacggggact ggatcgcgcg gaacaggata cgcgcggttt tgatgtcgtt ggccttcagg
     2281 cacaactcga tgatttcgcc cacgccggca tcccgcatca ggtcgtcaga cattttcatc
     2341 gcgatttcca tcgcggccct ggcagcaccc ttgtagcgct cgccgtcgtc cagatttttc
     2401 ttcgcgtcgc gctttttcct gctgtcggcg gggtggccga cgcttgcggc gctatcggcg
     2461 gcggaccggc agacatggcg aatcctttcc gcggcttcga tgtcgccgat cggccactgg
     2521 atagtctctt cccaggcttc cttcggcttc ttgcggctga accaccacat cgccttgccc
     2581 tgcaagcccc ggcaagcatg gtcatgtgca gtcgtcgtga ttgcaagccc tatcaggatg
     2641 cggttggctg cagtcggaag gccctcagct cgtgcgcttc ggcccgatcg cctcgaactg
     2701 cgccttctgc tcgtcgctca gcgtggcgta gaaatcatca agggccggcc gcaccagctt
     2761 gatcgcatcc aacagcgcat cgagccgcgt gtcgaccgag gcgagacggt ccggcggcaa
     2821 gatgccctcg tcgggctgac agtcgtaact caggatgtcg cgcgccttcg cgctcatgtg
     2881 ctgaagcacc gcaagctcgg cgcgctgggc gtcgtccagg tgcaaccggg cctcgatctc
     2941 gccgccgggc cactggaatg aagcgggctg ggcatcctga cagcttcgca ccggtgaggg
     3001 cttgggcgcc gccctctgct tgcgctgccc ctctccgaac gcttcgagct tgcctttctg
     3061 ctcgtcgtcc aggagctcat agaacctctc caacgccggc ctgaccctgg cgaccgcgga
     3121 gaccatcgct tcgagacgct gctgcatcgc agccaacctg agggacgccg tagctgccac
     3181 ctcgccggga cacacggcgc ggacgatctt tgctgcctcc accgatgcgc cggcgagatc
     3241 gtcaagcgcg gcactctgct cctcgttcaa ctggactacc tgccggatct gatcaacggc
     3301 agccgtggcc gcatcgtcct tcgcatcgcc gcacatcgat gcgacggaaa tgatcctgcc
     3361 gtgtcgtcgg ccgcgcgacg gcgccacata acgggtgagg tcgtcattgc catagggcgc
     3421 gaagaccgcg gcgtagagat caccgtagcc gtagccccag aagccgctgg tgtcgcccca
     3481 gacggtgtag tcgtagatgt cctggtaggc gaagggccag aacagcggac ccacccagcc
     3541 atagccgcca tcctcatgcc gccaccatcc gtgcgcgccg cggccgccat gccagccggc
     3601 caacgcggcg gatgcggcga gttgcacccg cgccgaggga ttggcggacc attcggcgtt
     3661 gcggaaccca tcggtgcggg acgttgtgtc gactgtgtcc cggacgctcg ccaacctcac
     3721 cgcgccgccg cgccgggcat agtgccgatg gtgcaggcct cgcagcggca acatgtgcgc
     3781 gaacgcggat ctgatgacgc cgaacggccc accgagacga atgcgcaagc cggcactcgc
     3841 gggctccgcg ctcgcgagcg cagttacggc gaccagaacg gcaacaccaa tgcggaatat
     3901 cgacatggca cctccccgca ttgcttcttc agtctcgtca acgatcagct cgttcgccgc
     3961 ggtcctatcg cctcgaattg cgccttttgt tcatcgctga gcgtcgcgta gaaatcctcg
     4021 agcgccgcgc gcaccgactt caccgcctgc agcatggcat cgagccgctt gccgacggcg
     4081 tcgagccgcg cgaccggcgt gatcgcctcc gcgggctggc aggccgcctt cagcgcgtcg
     4141 gcggcacggg ccgtggtgtc ctgaagcact tgaagcgcgg cgcgctggat atcgttcgga
     4201 tgcagcctcg cttcgatctc ggccgccggc caggcaagcc cggcaggttg cgcggcgctg
     4261 cagccctgcg cggcagggcc gttcgcattg tccggcgacc tcccgcgctg atcctcggcc
     4321 agcgcattga gccgcacctt ttgttcgtcg ttcagaagcg cgtagagcct ttcgaggggc
     4381 ggccgcacca gcccgaccgc ggaaatcatc gcctcgacgc gctgctccat ggcggcaagc
     4441 cgtgcgggcg ccgtcgccgc aacctgcgtc ggacacgacg cccgaatcat ttgcgctgcc
     4501 ttgatggagg cgtcggcgag atcgtcgagc gcggggcgtt gcgcttcggt cggctcgatc
     4561 gcctgcccga tctgatcgac gggaaggccc gcgatatcgc ggctgtcatc gctgcacatc
     4621 ctcgcgagcg agacggcgcc gcgatacctg cgaccgcccg acggaggcat catgtagccg
     4681 gagagttcgc catagccgta aggagcaaaa atgccggcat agatatccgg gtagccatag
     4741 ccccagaagc cgaagccgtc gccccagacc gca
//
LOCUS       DATLJT010000575         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_723014,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000575 DATLJT010000000
VERSION     DATLJT010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723014"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VM910_13330"
     CDS             complement(<1..407)
                     /locus_tag="VM910_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HVI63550.1"
                     /translation="MSEMPSLTARNPAGIGPGRLILVIGPSGAGKDTLLGLAKCACAD
                     DRNVIFPRRVVTRESSSSEDNDQLSPEDFQAGLERGEFAVHWEAHGHCYALPRSVEHD
                     IRAGRTVVVNVSRMVVDALRQVYADVAVVCITAP"
     gene            complement(404..1552)
                     /locus_tag="VM910_13335"
     CDS             complement(404..1552)
                     /locus_tag="VM910_13335"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HVI63551.1"
                     /translation="MISTKFETVIGNARIVLADRVIERGWIACADGRIAEIGEGNAPC
                     SFEDATGDLPGMIELHTDHLEAHYVPRPKVFWDPVAAVVSYDGQLATCGITTVLDSLR
                     VWREDGLDRDGQVGVVAAAIASAREADLLRADHFLHLRCEIPMPDVVDDARQLIDRPD
                     VRLMSLMDYTPGQRQFRDEAKLRDNYRGKGGGMTDAELDVLFERRSAYQKAYAAANMR
                     DIVALAHQYRIPLASHDDTTEENVGDAIRDRVAVAEFPTTIEAARGLHHAGIDILMGA
                     PNVVRGGSHSGNIAAVDLAREGLLDILSSDYIPSSLLMAALQLPQRVPAIDLASAVRT
                     VTKTPAEAVGLNDRGEIAPGKRADLIRVHVARDVPVVRSVWREGRRVA"
     gene            complement(1568..>2213)
                     /gene="phnL"
                     /locus_tag="VM910_13340"
     CDS             complement(1568..>2213)
                     /gene="phnL"
                     /locus_tag="VM910_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HVI63552.1"
                     /translation="QGGVRLPVVRGVTFQVKAGECVVLSGPSGAGKSSILKMIFGNYR
                     CDGGRIGIRHEGAMIDLANAEPRDILSVRRATIGYVSQFLRAIPRVPTIDVVAEPLIV
                     TGTSRADARQRAGHLLRRLNIPERLWTLPPSTFSGGEQQRVNIARGFISDHPVLLLDE
                     PTASLDAANREVVVDLIDEKKCAGVAIVAIVHDDEIRRWIADRVVDVTAFAAAA"
BASE COUNT          424 a          730 c          690 g          369 t
ORIGIN      
        1 ggcgcagtga tgcagaccac ggcgacgtcg gcatagacct ggcgcaacgc gtcgaccacc
       61 atacgcgaga cgttgacgac cacagtgcgg ccggcgcgga tgtcatgctc gaccgaacgc
      121 ggcagcgcat agcaatggcc gtgcgcctcc caatgcacgg caaactcacc gcgttcgagc
      181 ccggcctgaa aatcctccgg cgagagctga tcgttgtctt ccgacgacga agactcccgc
      241 gtcaccacgc gacgcggaaa gatgacgtta cgatcgtctg cgcaggcgca tttcgccaga
      301 cccaacaaag tgtccttgcc ggcgccactc gggccaatga ccagaatgag ccggcctggt
      361 ccgatgccgg ccggattgcg cgccgtcaag ctcggcatct cgctcatgcg acgcggcgcc
      421 cttcgcgcca gacgctgcgg acgacgggaa cgtcgcgggc aacatgcacg cggatcaggt
      481 cggcacgctt gcccggcgcg atctcgcccc ggtcgttgag accaacggct tcggccggcg
      541 tcttcgtgac ggtccgcacc gcggacgcca gatcgatggc ggggacgcgc tgcgggagct
      601 gcagcgcggc catcaacagg ctcgaaggga tgtagtcgga cgacagaata tccagcaggc
      661 cctcgcgtgc gagatcgacc gctgcgatgt taccggagtg cgagccgccg cgcaccacgt
      721 tgggcgcgcc catcaggatg tcgatgccgg catggtggag accacgagcg gcttcgatcg
      781 tggtggggaa ttccgcaaca gccacgcgat cacggatcgc atcaccgacg ttctcctcgg
      841 tcgtatcgtc gtggctcgca agcggaatgc ggtattgatg cgccagtgcg acgatgtcgc
      901 gcatattggc cgcagcatag gccttctgat aggcggagcg cctttcgaac agcacgtcga
      961 gctcggcgtc ggtcatgccg ccgcccttgc cgcgataatt gtctcgcagc ttggcttcgt
     1021 cgcggaattg ccgttgcccc ggcgtgtaat ccatcaacga catcagccgc acgtccggac
     1081 ggtcgatcaa ctgcctggcg tcatcgacaa cgtcaggcat ggggatttcg cagcgcagat
     1141 gaaggaagtg atcggcgcgc agcaaatcgg cctcgcgcgc gctggcgatc gcggccgcca
     1201 ccacgccgac ctggccatca cggtcaaggc catcctcgcg ccagacccgc agcgaatcca
     1261 gcaccgtggt gattccgcag gtcgcaagct gtccgtcata ggagacgacg gctgcgaccg
     1321 ggtcccagaa aaccttcggc cgcggcacat aatgcgcctc gaggtgatcg gtgtgcagct
     1381 cgatcatgcc gggaagatcg ccggttgcat cctcgaaact gcagggagcg ttcccctcgc
     1441 cgatttcggc aatacgaccg tcggcgcagg cgatccatcc gcgttcaatc acccggtccg
     1501 ccagaacgat cctggcgttg ccgatgacgg tttcaaattt cgtcgaaatc atctcacttg
     1561 ccttatgtca tgccgcggcc gcaaaggccg tcacatcgac cacacggtcg gcgatccagc
     1621 ggcgaatttc atcatcatgc acgatggcaa cgatggcgac gcctgcacac ttcttctcgt
     1681 cgatcagatc gacgaccacc tcgcgattgg cggcatcgag cgacgcggtc ggttcatcca
     1741 gcagcaggac cggatgatcg gaaatgaaac cgcgcgcgat attgacgcgc tgctgctcgc
     1801 cgcccgagaa ggtcgagggc ggcagcgtcc acagtcgctc gggaatgttg agacgacgca
     1861 gcagatgtcc ggcccgctgg cgggcgtccg cgcgtgaggt tccggtcacg attagaggct
     1921 cggcgacgac atcgatggtc ggcacacggg gtatggcacg caggaactgg ctgacatatc
     1981 cgatcgttgc gcggcgaacg ctcaggatgt ccctcggttc tgcattggcg agatcgatca
     2041 tcgcgccttc gtgccgaata ccgatacggc caccgtcgca gcgataattg ccgaaaatca
     2101 tcttcaggat cgacgacttc ccggcccccg acggcccgga cagcaccacg cactcgccgg
     2161 ccttgacctg gaaggtcacg ccgcggacga ccggcaacct tactccgccc tgc
//
LOCUS       DATLJT010000576         1677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2332070,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000576 DATLJT010000000
VERSION     DATLJT010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332070"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1183
                     /locus_tag="VM910_13345"
     CDS             104..1183
                     /locus_tag="VM910_13345"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HVI63553.1"
                     /translation="MASKLVVLCAALATFAHVSFASAEEAAPAKLPNVVILCTGGTIA
                     GTGATTTTTVGYNAAKIGCDTLVDSVPELKKVANMRGEQVYQIASESITNDNWLTLSK
                     RINQLLAQDGTDGVVVTHGTDTIEETAYFLDLTVKSKKPVVVVGAMRPATAISADGPI
                     NLYNAVILAGSKEAVGKGVLVCLNDEINAGREVTKTNTSTADTFRTPDLGMLGYMQDN
                     KPHFYRASTRPNTADTEFDVSGLDKLPQVDIVYAYGDYNSIALDAFVKAGAKGIVHAG
                     PGDGSVGAQMVKPLQDARTAGVVVVRSARVGSGIIARNGEQPDDKYDWVVSDDLNPQK
                     ARILLVLALTQTNDTKKIQEMFWKY"
BASE COUNT          353 a          500 c          525 g          299 t
ORIGIN      
        1 tggctcctgt ggtagacgaa ccatcagttc ctcagcgggc cgaccgtttc gcgcggaaag
       61 cgttgtagcc aacaactatt gagatagggc ggcggatgtg accatggcgt cgaaacttgt
      121 tgtcttatgt gcagcgctcg cgacgttcgc acacgtatct tttgcgtcgg cggaggaggc
      181 ggctccggcc aagctcccca acgtcgtcat cctttgcacc ggcggcacga tcgccggcac
      241 aggcgcgaca acgaccacca ccgtcggcta taacgccgca aagatcggtt gcgacacgct
      301 cgtcgatagc gtgccggaac tgaagaaggt cgcgaacatg cgtggcgagc aggtctatca
      361 gatcgcgagc gagagcatta ccaacgataa ttggctgacg ctctccaaac gtatcaatca
      421 attgctcgcg caggacggca ccgacggtgt cgtcgtgacg catggcacgg acacgatcga
      481 ggagacggca tacttcctcg acctcaccgt gaagagcaaa aagccagtgg tcgtggtcgg
      541 cgccatgcgt ccggctaccg caatcagcgc cgatggtccg atcaatctct acaatgcggt
      601 gatcctggcg ggcagcaaag aggccgtggg caaaggagta ctggtctgcc tcaatgacga
      661 aatcaacgct gggcgcgagg tcaccaagac caatacttcg acggcggata cgttccgcac
      721 acccgatctc ggcatgctcg gctacatgca ggacaacaag ccacatttct accgtgcgtc
      781 gacccgtccc aacacggccg acaccgaatt cgatgtttcc gggctcgaca agctgcccca
      841 ggtcgacatc gtctatgcgt acggcgacta taattcgatt gcactcgacg ctttcgtcaa
      901 agccggcgcg aagggtatcg tgcatgccgg tccgggcgac ggttcggtgg gcgcgcaaat
      961 ggtcaagccg cttcaggacg cgcgcacagc gggggtggtc gtcgtccgct cagcgcgggt
     1021 cggctcgggc atcatcgcgc gcaacggcga acagcccgac gacaaatacg attgggtcgt
     1081 cagcgacgac ctcaatccgc agaaggcccg tatcctgttg gtgcttgcgc tgacgcagac
     1141 gaacgacact aagaaaatcc aggagatgtt ctggaaatat tgagcctttt ttgagcggtt
     1201 cccagcccgc gctcggtgca ttctcacggg ccaagcgagg gccattgaca gcctactgct
     1261 cggcagaggt ctgcgcctgt ctccgggcaa ggtctcataa ggggcacgaa gagcggttcc
     1321 tgccgtcgag gctgagtgcc ggctttctgc tcagaaagga gaccatcgcc ggaaggcgcc
     1381 gcaatgggcg aaacgcgccg aagaacgaac ccgctggcgc gggagggcac gcgtgacggt
     1441 cggtgcgttt tgggcggagt aagcagtaag gcgtgacggt cggggcatcc gatcctgagc
     1501 atcgaaggtc cagcaacctt cagctcggga gagcctcgat gacctccgat acctcgcctc
     1561 ggcccatcat cagcccgctg cgggcgcgca tgatcgaaga catgagcgtg cgcggcttca
     1621 aggaggacac gcggcgcgat tatgtgcgcc atgtgcgggc attcgcggcc ttcattg
//
LOCUS       DATLJT010000577         8538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1642998,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000577 DATLJT010000000
VERSION     DATLJT010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642998"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VM910_13350"
     CDS             <1..591
                     /locus_tag="VM910_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI63554.1"
                     /translation="SQAKLDVHYVPGEHDFLDEEVKFYKDRYGKDTKGPGYYSFDAGG
                     AHFIGLVNVVDLKAGGLGNLGAEQLEWLEDDLKGKSKSTPIVVFAHIPLWTVYPQWGW
                     GTEDGAKALEYLKGFGSVTVLNGHIHQVMQKVEGNVTFHTARSTAFPQPAPGTAPSPG
                     PMKVEDSKLRGLLGIASVNFKQGDQRLAIIDTPLQG"
     gene            607..942
                     /locus_tag="VM910_13355"
     CDS             607..942
                     /locus_tag="VM910_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HVI63555.1"
                     /translation="MTFANLRGLGLALAIAAVALLQLGVAGARAQDINVTIDNFTFTP
                     PELNVKVGDTVTWTNHDDIPHTVVSAGKFRSKTMDTDNSFSFTFTSAGDYKYFCSLHP
                     HMTGMIKVE"
     gene            1034..1594
                     /locus_tag="VM910_13360"
     CDS             1034..1594
                     /locus_tag="VM910_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI63556.1"
                     /translation="MPASNSNDDPDRARRFREAALPHLDDVYTLARYLLRDAADAEDA
                     VQECYLRALKHFDSYRGPAMKPWLFAILRNVCRAEYARRASSPSSAIEDVPESAEQPP
                     LWHEEQETPEAQMLRERDATAIRRLIDTLAEPFRETFVLREINNLSYREIAEAVGTPV
                     GTVMSRLARARAMLRSAWLAEQEQPR"
     gene            1591..2355
                     /locus_tag="VM910_13365"
     CDS             1591..2355
                     /locus_tag="VM910_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVI63557.1"
                     /translation="MNCDEARILLHALIDGELDASHAREVEAHIATCPACGAELAAYR
                     ELHEKIASADLRFTAPPLLRKRIEAALPKPQAAPSRRSVLRGFALGSAVSAMAATGLV
                     AIVLRNDDEQRIESEIVSAHLRSLQAGHLTDVVSSDQHTVKPWFNGRLDVAPPVIDLT
                     AQGFALIGGRLDFVDGRALGAVVYRRRAHIINLFVAQTASTEHRAAKIDSVQGFNIRR
                     WSDRGLNYWAISDIGADELSEFGEKFEAAARINSEG"
     gene            complement(2363..3754)
                     /gene="hpnO"
                     /locus_tag="VM910_13370"
     CDS             complement(2363..3754)
                     /gene="hpnO"
                     /locus_tag="VM910_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobacteriohopanetriol synthase HpnO"
                     /protein_id="HVI63558.1"
                     /translation="MLSPNLDVSEMFVERQAQRSSMHARHLNEQLVRVLKTIGYDVGF
                     QKGQGQYLFDRDGARYLDLLSGFGVFAIGRNHPALRTALKSVLDSDLPNLVQLDVSTL
                     AGVLAEKLIERVPYLDKVFFANSGAETVEASIKFARGATGRPGIVYCSHAYHGLSYGA
                     LSLTDDPNFRSGFEPLLPGCTAIPFNDLAALEHALSSRQVAAFIVEPIQGKGVNMPTD
                     EFLPGAAALCRKYGTLFIADEIQTGMGRTGRFLAVEHWNVEPDMVLLSKALSGGHVPV
                     GALLTRKSIFDKIFNQMERAVVHGSTFAKNDLAMAAGIATLEVLKSERLIEAAAKRGA
                     ELRLALTRMVPGYELMKEVRGKGLMIGVEFGPPKSLRLKASWNVLETASKGLFCQLIT
                     VPLFKDHKILTQVSGHGSHTIKLLPSLTITEEDCNWIERAFDSVIASSHKVPGAIWSL
                     GKTLVDNAVRKSA"
     gene            3936..4460
                     /locus_tag="VM910_13375"
     CDS             3936..4460
                     /locus_tag="VM910_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI63559.1"
                     /translation="MAADPTGDWRVADGVANIRVAQCNGNMWGVVAWEQSPGGHDVNN
                     PDVSKRNRPTLGMPILLDMKKKPGADQWEGQVYNAKDGKLYSSTITPTSPEQLEIRGC
                     VLGFLCGGETWTRVAPPIPSSPANSMAKGAPKTIGAAKTTPPNPAAKTASHGQSADAV
                     GDICLLPDIARLSH"
     gene            complement(4428..7016)
                     /locus_tag="VM910_13380"
     CDS             complement(4428..7016)
                     /locus_tag="VM910_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVI63560.1"
                     /translation="MLTSVVVSIVRTCTRFATLTLLAGLLLAIGAGYYTAHHFAINTD
                     INTLISDKLDWRQRDVQFDRAFDRDSTILAVVEAPTPELTNLAATALEQKLKGDTKNF
                     LSMQALGSGEFFEKNGLLFLPTQELAELAGQFESAAPLVEIMAGDPSIRGLTGALETG
                     LAGVQRGEVKLDNTVRPFNLIAQTVEDILSKGSANFSWRELTSKTPLTDSDRRAFIEF
                     KPVINYGALEPGKEATDAIRKAAAELDFPQKFQARVRLTGPIPIANEEYSTVQEGAIV
                     NAIGTIVVVLFILWLALHSAKIIFAVFVNLFIGLSITTAVGLWMVGSLNLLSIAFAVL
                     FIGLGVDFGIQFSVRYRSERFKNDDLAQALEEAARRSAIPLSLAAMATAAGFLCFLPT
                     DYKGISELGEIAGAGMLIAFTSSITVLPALLRLLNPPGEKEPVGYAFLAPLDRFLEDH
                     RIPIVVGTLALVIAGLPLLYFMRFDFNPMNLRSPNAESIATYLDLRKDPNTGASAINV
                     LTHSDADAQKIAAKLEKLPEVLRVQWLDSFVPEDQPAKLKLLAQAARVLGPALSPESI
                     DDAPTDQENVDSLKSSVASLRKVAGEGNGPGAVASRRLADALQKLADSNEATRNKAQA
                     IFVDPLEVLLDQLKNALQAQPVSLNTLPPEIVSSWKAKDGLMRVQALPRGDPNDNDNL
                     RRFASAVLSAEPTAVGGPVSILKSGDTIVRAFILAGIYALVVISLLLWVTLQRVTDVL
                     MTLVPLLVAGAVTLEICVLIKLPLNFANIVALPLLLGIGVAFKIYYVVAWRSGRTNLL
                     QTSLTRAIFFSAMTTATAFGSLWLSSHPGTSSMGKLLALSLVTTLAAVLLFQPALMGK
                     PRDIGQ"
     gene            7440..8366
                     /gene="ispH"
                     /locus_tag="VM910_13385"
                     /gene_synonym="lytB"
     CDS             7440..8366
                     /gene="ispH"
                     /locus_tag="VM910_13385"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVI63561.1"
                     /translation="MEVYLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKY
                     VVESLKNKGAVFVEDLSEVPPHSVTVFSAHGVARTVEEEAAARGLPVLNATCPLVTKV
                     HNQGKRYMSKGRALILIGHAGHPEVEGTMGQVPGPVLLVQDVEDVAALTLAAETPVAY
                     ITQTTLSVDDTKDIITALHARFTDIQGPDIRDICYATQNRQSAVRDLSKLVDVILVVG
                     AANSSNSNRLREIGTEAGVPSYLIADGSELNPEWLRNTKTVGITAGASAPEVLVDDVI
                     EALRRIGPVKVSVLPGREENIEFRLPAELAAG"
     gene            8401..>8538
                     /locus_tag="VM910_13390"
     CDS             8401..>8538
                     /locus_tag="VM910_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnH"
                     /protein_id="HVI63562.1"
                     /translation="MAIPFFKEMRIGGYLIKQRLLGRKRYPLVLMLEPLFRCNLACAG
                     CG"
BASE COUNT         1710 a         2627 c         2660 g         1541 t
ORIGIN      
        1 tcgcaggcca agctcgacgt gcactatgtg ccgggcgagc acgatttcct cgacgaggag
       61 gtcaagttct acaaggaccg ttacggcaag gacaccaagg gccccggcta ctattccttc
      121 gacgcaggcg gcgcgcactt catcgggctc gtcaatgtcg tcgacctcaa ggccggcggt
      181 ctcggcaatc tcggtgccga gcagcttgaa tggctcgaag acgatctcaa gggaaagtcg
      241 aaatcgaccc ccatcgtggt gttcgcgcat atcccgctct ggacggtcta tccgcaatgg
      301 ggctggggca ccgaggacgg cgcgaaggcg ctggaatatc tcaagggctt cggctcggtc
      361 acggtgttga acggtcacat ccatcaggtg atgcagaagg tcgaaggcaa cgtcaccttc
      421 cacaccgccc gctcgaccgc gttcccgcaa cccgcgccgg gcaccgcgcc ctcgccggga
      481 cccatgaaag tggaggattc caaattgcgc ggcctgctcg gcattgccag cgtcaacttc
      541 aagcagggcg accagcgcct cgcgatcatc gacacgccgc tgcaaggctg aagggaagtt
      601 cacaaaatga cgttcgccaa tctgcgcggc cttggccttg ccctggccat cgccgcggtc
      661 gccctcctgc aactcggcgt cgccggggcg cgggcccagg acatcaacgt gacgatcgac
      721 aatttcacct tcacgccgcc cgagctgaac gtaaaggtcg gcgacaccgt gacctggacc
      781 aaccacgacg acatcccgca cacggtggtg tcggcgggca aattcaggtc gaagaccatg
      841 gataccgaca actcgttttc gttcactttc acctcggcgg gtgactacaa gtatttctgc
      901 tcgctgcacc cgcacatgac cggaatgatc aaggttgagt agcggccaaa tccaaggcat
      961 cacaatgggg cccgggcggt tgttgacaac cggccgggca cacgcatttt ccgctgaagc
     1021 ggggcaagag gcgatgccgg caagcaattc caacgacgac cctgaccggg cgagacgttt
     1081 tcgcgaggcc gcgctgccgc atctggacga cgtctatacg ctcgcgcgct atctcctgcg
     1141 cgatgcagcc gacgccgagg acgcggtgca ggaatgttac ctgcgcgcgc tgaaacattt
     1201 cgacagttat cgcgggccgg cgatgaagcc atggctgttc gcgatcctgc gcaacgtctg
     1261 ccgcgcggaa tacgcgcgcc gagcctcctc gccgagcagt gcgatcgagg acgtgccgga
     1321 gtccgcggaa cagccgccgc tgtggcacga agagcaggaa acgccggaag cgcagatgct
     1381 gcgcgaacgc gacgcgaccg cgatcaggcg gctgatcgat acgctggccg aaccctttcg
     1441 cgagaccttc gtgctgcgcg agatcaacaa tctgtcatac cgcgagatcg ccgaggcggt
     1501 cgggacaccg gtcggtaccg tgatgtcccg gctcgcgcgt gcccgcgcca tgctgcgctc
     1561 ggcatggttg gcggaacagg agcaaccgag atgaattgcg acgaggccag aatccttctc
     1621 cacgcgctga tcgacggcga actcgacgcg tcgcacgcgc gtgaggtcga agcgcatatc
     1681 gcgacctgcc ccgcctgcgg ggccgagctc gcagcctatc gcgagctgca cgaaaagatc
     1741 gcctccgccg atctgcgttt taccgcgccg cccctgcttc gcaagcgtat cgaagcggcg
     1801 ctgccgaagc cgcaagccgc gccgagccga cgctcggtgc tgcgcggatt tgcgttggga
     1861 tcggcggtgt cggcgatggc ggcgaccggt cttgttgcca tcgtgctgcg taacgacgac
     1921 gaacagcgca tcgaatcgga gatcgtctcg gcgcatctgc gctcgttgca ggccggccat
     1981 ctcaccgacg tggtttcaag cgaccagcac acggtgaagc cgtggttcaa cggcaggctc
     2041 gacgtcgccc cgcccgtgat cgatctcact gcgcagggat ttgcgctgat cggcggccgg
     2101 ctcgatttcg tcgacggccg cgccttgggc gcggtggtct atcgccgccg ggcgcatatc
     2161 atcaatctgt tcgtggcgca gaccgccagc acagagcatc gcgcggcgaa gatcgacagc
     2221 gtgcagggtt tcaacatccg ccgctggagc gatcgtggcc tgaattactg ggcgatcagc
     2281 gatatcggcg ccgacgaatt gtccgaattc ggggaaaagt tcgaggcggc ggcgcggatc
     2341 aacagcgagg gatgatgcgc gatcaggccg acttgcgcac ggcgttgtcg accagcgtct
     2401 tgccgagcga ccagattgcg ccggggacct tgtggctcga ggcgatcacg gaatcgaagg
     2461 cccgctcgat ccagttgcag tcctcttcgg tgatggtcag cgagggcaac agcttgatgg
     2521 tgtggctgcc gtgacccgag acctgcgtca ggatcttgtg atccttgaac agcggcacgg
     2581 tgatgagctg gcagaacagg cccttgctcg cggtctccag cacgttccag gacgccttca
     2641 gccgcagcga cttcggcggg ccgaactcga cgccgatcat cagccccttg ccgcggacct
     2701 ctttcatcag ttcgtagccg ggcaccatgc gcgtcaacgc gagcctgagc tccgcaccgc
     2761 gcttggcggc cgcctcgatc agccgttcgg acttcagcac ttccagcgtc gcgatgccgg
     2821 ccgccatcgc gagatcgttt ttcgcaaagg tcgaaccatg cacgaccgcg cgttccatct
     2881 ggttgaagat cttgtcgaag atgctcttgc gcgtgagcag cgcgccgacc gggacatgtc
     2941 cccccgacag cgctttcgac aacagcacca tgtcgggttc gacattccag tgttcgacgg
     3001 cgagaaaccg ccccgtgcga cccatgccgg tctggatttc gtcggcgata aacagggtac
     3061 cgtatttgcg acaaagcgcg gccgcccccg gcaggaattc gtcggtcggc atgttgacgc
     3121 ccttgccctg gatcggctcg acgatgaagg cggcgacctg gcgcgacgac agcgcgtgtt
     3181 cgagggccgc aagatcgttg aaaggaatcg ccgtgcagcc gggcaagagc ggctcgaacc
     3241 cgctacggaa gttgggatcg tccgtgagcg acagcgcgcc gtaggacagg ccgtgatagg
     3301 catgcgaaca atagacgata cctggccgtc cggtcgcgcc gcgggcgaac ttgatggacg
     3361 cctcgaccgt ttcggcgccg gaattggcga aaaatacctt gtcgagataa ggcacgcgct
     3421 cgatcagctt ctcggcgagc acgcccgcca gcgtcgagac gtcgagttgc acgagattgg
     3481 gcagatcgct gtccagcacg cttttgaggg cggtccgcag cgccgggtga ttgcgcccaa
     3541 tcgcaaaaac accaaaaccg ctcaaaagat caagatagcg cgcgccatcc cgatcgaaca
     3601 ggtattgtcc ctgccctttc tggaacccca catcgtagcc gattgttttg agaacgcgga
     3661 ccaattgctc gttcaaatgg cgcgcatgca tggaactgcg ctgcgcctgc cgttccacaa
     3721 acatctcgga aacgtctaga ttcggactta gcatcagcta catacttcgg ttgatagccg
     3781 tctcgtcaac tgaaaacgcc caatgcggcg ttttgccctc attcacacca tcaacgcaag
     3841 cacaggtttg agccatgttg caatgcccaa gaaacttgct ctggatagca cttttttccg
     3901 ccgcaatatt cgcggcaagc ggtgcacctt gcgcgatggc ggccgatccg accggcgatt
     3961 ggcgggtggc ggacggcgtc gccaacatcc gggtggcgca atgcaatggc aacatgtggg
     4021 gcgtggtggc gtgggagcag agcccgggcg gccacgacgt taacaatccg gacgtctcga
     4081 aaagaaacag gcccactttg ggcatgccga tattgctcga tatgaagaaa aagcccggcg
     4141 ccgatcaatg ggaaggccaa gtctacaacg ccaaggacgg caaactctac agttcgacca
     4201 ttacccccac gagccccgag cagcttgaaa ttcgcggctg cgtgctcgga tttctctgcg
     4261 gtggcgagac ctggacgcgg gtcgccccgc cgatcccctc aagcccggcg aacagcatgg
     4321 ccaagggcgc acccaagacg atcggcgcgg ccaaaaccac gccgccgaat ccggccgcca
     4381 agacggccag ccacggacaa tcggccgacg ccgtcggcga tatctgtcta ttgcccgata
     4441 tcgcgcggct ttcccattag ggccggctgg aacaagagca ccgcggcgag cgtggtgacc
     4501 agcgatagcg cgagcaattt gcccatgctc gaggtcccgg ggtggctgga cagccagagg
     4561 cttccgaacg ctgtcgccgt cgtcatcgcg ctgaaaaaga tcgcacgcgt cagactggtt
     4621 tgcagcagat tcgtcctgcc cgagcgccag gccacgacat aatagatctt gaaggcgacg
     4681 ccgatgccga gcagcagcgg caaagcgacg atgttggcga aattgagcgg caatttgatc
     4741 agcacgcaaa tttccagcgt caccgcgccg gcgaccagca gcggcaccag cgtcatcagc
     4801 acgtcggtca cccgctgcag ggtcacccac aacagcaggc tgatcaccac cagcgcgtag
     4861 attccggcga gaataaaggc gcgcacaatg gtgtcgccgg atttgaggat cgataccggg
     4921 ccgccgaccg cggtcggctc tgccgacagc actgcgctgg caaatctgcg caggttgtcg
     4981 ttgtcgttgg ggtcgccgcg cgggagtgcc tgcacccgca tcaacccgtc cttcgccttc
     5041 caggaggaaa cgatctcggg aggcagggtg ttcaggctga cgggttgcgc ctgcagtgcg
     5101 ttcttgagct gatcgagcaa gacctcgagc ggatcgacga agatcgcctg cgccttgttg
     5161 cgcgtcgctt cattggagtc ggcaagcttt tgcagcgcgt cggcaagccg tcgtgaggcg
     5221 accgcgccgg gtccgttccc ttcgcctgcg actttgcgca ggctcgcgac cgagctcttc
     5281 agcgagtcga cattctcctg atccgtcggc gcatcgtcga tggactccgg gctgagcgcg
     5341 gggccgagca ccctggcagc ctgcgcaagc aatttgagct tggccggctg atcttccggc
     5401 acgaagctgt cgagccactg gacccgcaag acttccggaa gcttctccaa ctttgctgca
     5461 atcttttggg catcggcatc ggagtgggtc agcacattga tggcgctcgc gccggtgttc
     5521 ggatccttcc gcagatcgag ataggtcgcg atcgattcag cgtttggact acgaaggttc
     5581 atggggttga agtcgaaccg catgaaatac agcagcggca gccccgcgat caccaacgcc
     5641 agcgtaccga cgacaatggg aatgcgatga tcctcgagga agcggtcgag cggcgccagg
     5701 aacgcgtagc ccaccggctc cttttctccg ggcggattga gcagcctcaa caaagcgggc
     5761 agcaccgtga tgctcgaggt gaacgcaatg agcatgccgg cgccggcgat ctcgccgagc
     5821 tcggagatgc ccttgtagtc ggtcggcaga aagcacagga atccggccgc ggtcgccatc
     5881 gccgccagcg acagcggaat ggcggagcgg cgcgccgcct cttccagcgc ctgcgccagg
     5941 tcgtcattct tgaagcgttc cgagcgatag cggacgctga actggatgcc gaagtcgacc
     6001 ccgagaccga tgaacaatac cgcaaacgcg atggacagca ggttcagcga gccaaccatc
     6061 caaagcccca cagccgtcgt gatcgacagg ccgatgaaga gattgacaaa cacggcgaag
     6121 ataatcttcg ccgagtgcaa cgccagccac agaataaaca gcaccaccac gatggtgccg
     6181 atcgcgttga cgatcgcgcc ctcctggaca gtcgaatatt cctcatttgc gatcggaatc
     6241 ggcccggtca ggcggacgcg cgcctggaat ttctgcggga aatccagctc ggcggcggcc
     6301 ttgcggatcg catcggtcgc ctccttgccg ggctcgagcg cgccgtagtt gataaccggc
     6361 ttgaattcga tgaatgcgcg gcgatcggaa tcggtcagtg gggtcttact ggtcagctcg
     6421 cgccaggaga aattggcgct tcccttgctg aggatgtcct ccaccgtctg cgcgatcaga
     6481 ttgaacggcc tgaccgtgtt gtcgagcttg acctccccgc gctgcacgcc ggcaagcccg
     6541 gtttccagcg cgccggtcaa tccgcggatc gaggggtcgc cggccatgat ctcgaccagc
     6601 ggtgccgcgg actcgaattg tccggcaagc tctgccagtt cctgggtggg caaaaacagc
     6661 aacccgttct tttcgaagaa ctcgccagac ccgagcgcct gcattgacag gaaattcttg
     6721 gtgtcgcctt tgagcttctg ctcgagggcg gtcgcggcca agttcgtcag ttccggggtc
     6781 ggcgcctcga ccaccgcaag gatggtgctg tcgcgatcga acgcccggtc gaattgcacg
     6841 tcgcgctgac gccagtccag cttgtccgaa atcagcgtgt tgatgtcggt attgatcgcg
     6901 aaatggtggg cggtgtagta gcccgccccg atcgcgagga gcagtccggc cagcagcgtg
     6961 agagttgcaa aacgggtaca cgttctgacg atcgagacga cgacacttgt cagcacatca
     7021 attctttcta atgttgatag cttgccggaa acatcccgct gtctagcgga gttccggagg
     7081 ccgggctaac cttgttttcg acgatttttt tcctagggaa cacaggtaca tagcccccaa
     7141 aacagggtgg gatcagcgcg gtggctctat cgctggcaat cttgggcgta aagtgacgaa
     7201 ggagtgaggg gctaaacact tcatctggct tggctaatcg gatagtatcc tgttaccaac
     7261 gagacctcca aaggcgggtc gcgaacgggg aggcttttcc ggcggatata tttttgacac
     7321 aaagccttgt atctccagcg ctaaccggcg cggaaccgag gctgagagcc gcggtgcgga
     7381 tattgcagct attccacatc gtgggagtta ggtaaggcaa attgagtaac ggacgtccca
     7441 tggaagtcta cttagcgcag ccacgaggtt tctgcgccgg cgtggtgcgc gcgatcgaga
     7501 tcgtcgagcg tgccctcgag aaatatggtc cgccggtcta tgtgcggcac gagatcgtgc
     7561 acaacaaata cgtggtcgaa agcctaaaga acaagggcgc ggtcttcgtc gaggacctct
     7621 ccgaggtgcc gccgcattcg gtgaccgtgt tcagcgctca cggcgtggcg cggaccgtcg
     7681 aggaagaggc cgccgcgcgg ggcctgccgg tcctcaatgc cacctgtccg ctggtcacaa
     7741 aagttcacaa tcaaggcaag cgctacatgt ccaagggccg ggccttgatc ctgatcggcc
     7801 atgccggcca tcccgaggtg gagggcacga tgggccaggt tcccggcccc gtcctgctcg
     7861 ttcaggatgt cgaagatgtg gcagcgctca cgctggcggc ggagacgccg gtcgcctata
     7921 tcacccagac cacgctttcc gttgatgata ccaaagacat cataacggca cttcacgcgc
     7981 gatttacaga tattcaaggt ccggacatcc gggatatctg ctatgcgaca cagaaccgcc
     8041 aatctgcggt aagggacctg agcaagctcg tcgacgtgat cctggtggtg ggcgccgcca
     8101 atagttccaa ttcaaaccgg ctgcgcgaga tcggcaccga ggccggtgtc ccgagttatc
     8161 tcattgctga cggcagcgaa ctcaacccgg aatggttgag gaacaccaaa actgtcggca
     8221 tcacggccgg cgcttctgcg ccggaggtcc tggtggacga cgttatcgag gccctgcgac
     8281 gcatcggacc ggtcaaggta tcggtgctgc cgggccggga ggaaaacatc gaattccggc
     8341 tgcccgcgga actcgcggcc gggtgacaac aactcgagct aaaggtgttg aaagcacata
     8401 atggctatac cgttcttcaa ggaaatgcgt atcggcggct atctgatcaa gcagagattg
     8461 cttggccgga agcgctaccc gcttgttctg atgctggagc cgctgttccg ttgcaacctc
     8521 gcttgcgccg gttgcggc
//
LOCUS       DATLJT010000578         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_953207,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000578 DATLJT010000000
VERSION     DATLJT010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953207"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VM910_13395"
     CDS             complement(<1..1458)
                     /locus_tag="VM910_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HVI63563.1"
                     /translation="MSAQANTARSLDTHPLRRISFGDPVVSIEQAGDGTIYLRPSTPL
                     GDYPRRLTDRLHHWASEAPDRTFMAERVAGGGWRQVSYGELLTASRYIASALLARELS
                     ADRPVVILSGNSIDHALVAFGALYAGIPFCPVSPAYSLVSRDYGKLAYVMKLLTPGLV
                     FADDADKFSAALANVPQGTEIAASFGAVPGRDVTELAHLLSAPLHPDLDAVHEAIGPD
                     TIAKFLLTSGSTGSPKAVINTQRMLCANQVMVRETMSFLKDEPPVIVDWLPWSHTFGG
                     NHNIGLTLFNGGSMYLDQGKPMPGGIEETVKNLREISPTVYFNVPKGYESLLPYLRDD
                     AGLRGKFFARLHAMFFAGAALSPFVWNSLDELSVRETGQRVPMLTGLGSTETAPFFMS
                     VNPATSRSGHIGLPVSGCDAKLAPVNGKLEVRARGPNITPGYWRQPELTKAAFDEEGF
                     YRLGDALRPANAQDLHAGFDFDGRIAEDFKLASGTW"
     gene            complement(1455..2264)
                     /locus_tag="VM910_13400"
     CDS             complement(1455..2264)
                     /locus_tag="VM910_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVI63564.1"
                     /translation="MGNAVSAASAEASALLKVEQQGKVLTVGLNRPAKRNALNDGIIL
                     AIGDCFAHLPENIGAVVIHGIGDHFSSGLDLSELTEHDATEGLLHSQMWHRVFDRIQY
                     SRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLI
                     GVARMTDMMLTGRVYSATEGSSYGFAQYLVEAGGALAKALELAERIASNAPLTNFAVL
                     QALPMIAEANPQTGLLMESLMATVAQSDKEAKRRIRQFLDHKTAKVKPGKS"
BASE COUNT          412 a          718 c          749 g          387 t
ORIGIN      
        1 ccaggtgccg ctcgcaagct tgaagtcctc ggcaatgcgg ccgtcgaaat cgaagccggc
       61 gtgaagatcc tgtgcattcg ccggccgcag cgcgtcgccg agcctgtaaa atccttcttc
      121 gtcgaaggcg gcctttgtca gctccggctg gcgccaatag cccggcgtga tgttcggccc
      181 ccttgcacga acctcgagct tgccgttgac cggcgccagt ttcgcgtcac agccggacac
      241 gggaaggccg atatggccgg agcggctggt tgccgggttg accgacatga agaacggcgc
      301 ggtttcggtc gagccgaggc cggtcagcat cggcacgcgc tggccggtct ctctgaccga
      361 caattcgtcg aggctgttcc agacgaaggg cgagagcgcg gcgcctgcga agaacatcgc
      421 gtgcaggcgc gcgaagaact tgccgcgaag ccccgcgtcg tcgcgcagat acggcaacag
      481 cgattcgtaa cctttcggca cgttgaaata gaccgtgggc gagatctcgc gcaggttttt
      541 cacggtctcc tcgatgccgc caggcatcgg cttgccctga tcgagataca tcgagccgcc
      601 attgaagagc gtcagcccga tgttgtggtt gccgccgaag gtatggctcc acggcagcca
      661 gtcgacgatc acgggcggtt cgtccttgag gaaggacatg gtctcacgga ccatcacctg
      721 attcgcgcag agcatccgtt gcgtgttgat caccgccttg gggctgccgg tcgagcccga
      781 ggtgagcagg aatttcgcga tcgtatcggg gccgatcgct tcatggaccg cgtcgagatc
      841 gggatgcagc ggcgcgctca ggagatgggc gagttctgtc acgtcgcgtc cgggcacggc
      901 gccgaacgag gccgcgatct cggtcccctg cgggacgttg gcaagtgcgg cggaaaactt
      961 gtcggcgtcg tcggcgaaca cgagacccgg cgtcaacagc ttcatcacgt aagcgagctt
     1021 gccgtagtcg cgcgacacca gcgaataggc cggcgacaca ggacagaacg ggattccggc
     1081 atagagcgcg ccgaacgcca cgagcgcatg gtcgatcgaa ttgccggaaa ggatcacgac
     1141 cggccgatcg gccgacagct cccgcgccag cagggccgag gcaatgtagc ggctcgcggt
     1201 caagagctcg ccatagctga cctgtcgcca gccgccgccc gcgacacgct ccgccatgaa
     1261 ggtgcggtcg ggcgcctcgc tcgcccaatg atgcaggcga tcggtcaggc ggcgcggata
     1321 gtcgccgagc ggcgtgctcg gacgcagata gatcgtgccg tcgcccgcct gctcgatcga
     1381 tacgacggga tcgccgaacg agatcctgcg cagcggatgg gtatcaaggc tccgcgctgt
     1441 gttggcctgt gcgctcatga tttgcccggc tttactttgg cggtcttgtg gtcgaggaac
     1501 tggcgaatgc gccgcttggc ttccttgtcg ctttgcgcga cggttgccat cagcgactcc
     1561 atcagaaggc cggtttgcgg attggcttcg gcgatcatcg gcagcgcctg cagcacggcg
     1621 aaattggtca gcggcgcatt ggaagcgata cgctccgcaa gctcaagggc ctttgccagc
     1681 gcgccgcccg cctcaacgag atactgcgca aagccgtaag acgagccttc ggtcgcggaa
     1741 tacaccctgc ccgtcagcat catatcggtc atccgcgcga cgccgatcag ccgcggcaaa
     1801 cgcaccgatc cgccgccgcc cacgaaaatg ccgcgctggc cttccggcag cgcgaaatag
     1861 gccgacggct cggcgacgcg gatatgcgcg gcgcaggcaa gctcgagccc gccgccaatg
     1921 accgcgccct tcagggctgc gatcacaggc acgcggctat actggatgcg gtcgaacacg
     1981 cggtgccaca tctgcgaatg cagcaggccc tcggtcgcat cgtgctcggt cagctccgag
     2041 agatcgaggc ccgaggaaaa gtgatcgccg atgccgtgaa tcacgacggc accgatattt
     2101 tccggcagat gggcgaagca atcgccgata gcgagaataa tgccgtcgtt caacgcgttg
     2161 cgcttggccg gccggttcag gccgacggtc aggactttgc cttgctgctc gaccttgagc
     2221 agcgcggaag cttcggcgct cgcggcgctg acggcgtttc ccatgg
//
LOCUS       DATLJT010000579         3234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_953210,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000579 DATLJT010000000
VERSION     DATLJT010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953210"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VM910_13405"
     CDS             <1..1504
                     /locus_tag="VM910_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HVI63565.1"
                     /translation="ESWLTKHLKPGDRLGFDPWLHTSSAAERLAAACTKTGAELVAVD
                     SNPVDAIWTERPQPPLAPVAIHGPQFSGETEADKLNRIRSEIDKLGVDALVFSDSHAV
                     AWTFNIRGADVAHTPLPLSYALVPKDGRPTVFIDHRKLTNATRDHLEQSAEVREPDAL
                     AGELAALAKAGGSIALDNATAADALTRLISAAGGKAVRGSDPVALLKAMKNPTEIAGT
                     RTAHRRDAVALARFLAWVAREAPKGRLTEIDAVEALETFRRDTGALKDVSFPTIAGTG
                     PNGAIVHYRVTRKSNRRIVPGDLLLVDSGAQYEDGTTDVTRTIAVGEPTGEMRDRFTR
                     VLRGHIAIASAVFPDGTAGAQLDTLARQFLWQAGIDFEHGTGHGVGSYLSVHEGPARI
                     SKLGTTPLKRGMILSNEPGYYKTDGYGIRIENLELVIAEDVAGAEKPMNAFETLTLAP
                     IDRRLIEVAMLSPAERDWLDAYHARVRIEVRSHLDETDKLWLDAATAPLT"
     gene            1635..2912
                     /locus_tag="VM910_13410"
     CDS             1635..2912
                     /locus_tag="VM910_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HVI63566.1"
                     /translation="MHGVMGKLPPTATKNVAASKLTLLLLVAMTGVAPISLYMLVPAL
                     PKLATAFGRDISLTQMTVSLYMVGIACSQLVMGPLSDRFGRRPVLLSGLALMVVASAA
                     CIFAQNLPQLIIARFLQALGGASGMVISRAIIRDLYERDQIGAKISLVIAVMMIAQML
                     SPLTGGLVETAFGWRAIFYLITAAVLVTVIAIVLLLPETRRGRLPGNGFRGDFGGLFK
                     SRAFVGFMLCQVLASQIIFAFAGGGPYIVVTQMSRTSAEYGAWFATTGFAYLIGNLFC
                     VRFAPRHSLERLIWFGLALQFVGSALNLAWSITGINQAPSFLFGTQMIVMFANAFVMS
                     NSAAGAISVRPDAAGTASGAMGFLQMGIGSLVSQFGAYLGGHFATTLPLTAFIFALSL
                     ACASTMIFLVPRRAVIVSEELMEKAEEEESGML"
     gene            complement(2961..>3234)
                     /locus_tag="VM910_13415"
     CDS             complement(2961..>3234)
                     /locus_tag="VM910_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HVI63567.1"
                     /translation="EEVTILDAERPRSSSAISGKTVVFTGSLEKMTRDEAKAMAERLG
                     AKAAGSVSKKTDYLVAGPGAGSKLAEARKHGVAVLTEDEWLKMVGE"
BASE COUNT          544 a         1115 c          998 g          577 t
ORIGIN      
        1 ggaaagctgg ctcacaaagc acctcaagcc cggcgaccgc ctcggatttg atccgtggct
       61 gcacacatct tcggcagccg agcggctggc ggccgcctgc accaaaacgg gtgccgaact
      121 ggtcgcggtc gacagcaacc cggtcgatgc gatctggacc gagcggccgc aacccccgct
      181 tgcgccggtc gccatccacg gcccgcaatt ttccggcgaa accgaggcgg acaagctcaa
      241 ccgcatccgc agcgagatcg acaagctcgg cgtcgatgcg ctggtgttct cggacagcca
      301 cgccgtggcc tggaccttca acatccgcgg cgccgacgtt gcacacacgc cgctgcctct
      361 gtcctatgcg ctggtgccga aggacggccg gccgacggtg ttcatcgatc accgcaaact
      421 gacgaacgcg acccgcgacc acctggaaca gtccgccgag gtcagggagc ctgacgcgct
      481 ggcgggcgaa cttgcggcgt tggcgaaagc cggcggctcg atcgcgctcg acaatgcgac
      541 cgccgccgac gcgctgaccc ggctgatcag cgccgcaggc ggcaaggcgg tgcgcggcag
      601 cgacccagtc gcgctcctca aggcgatgaa gaacccgacc gagatcgcag gcacgcgaac
      661 ggcgcaccgg cgcgacgcgg tcgcgctcgc ccgtttcctg gcctgggtcg cccgcgaggc
      721 gccgaagggc aggctgaccg aaatcgacgc ggtagaggcg ctggagacgt tccgccggga
      781 caccggcgcg ctcaaggacg tgtcgtttcc gaccatcgcc ggcaccgggc cgaacggagc
      841 gatcgtacac taccgcgtca cccgcaagag caaccggcga atcgtgccgg gcgacctctt
      901 gctcgtcgat tccggcgcgc aatatgagga cggtaccacc gacgtcaccc gcaccatcgc
      961 ggtcggcgaa cccaccggcg aaatgcgcga ccgcttcacc cgcgtgctgc gcgggcatat
     1021 tgcgatcgcc agcgccgttt tccctgatgg caccgcgggc gcgcaactcg acacgctggc
     1081 gcgccagttt ttgtggcagg ccggcatcga tttcgagcac ggcaccggtc acggcgtcgg
     1141 cagctatctc tcggtgcatg aagggccggc gcgaatctca aagctcggca ccacgcctct
     1201 caagcgcggc atgattctgt ccaacgagcc cggctactac aagaccgacg gctacggcat
     1261 ccggatcgag aatctggagc tcgttatcgc ggaggacgtt gcgggcgccg aaaagccgat
     1321 gaacgcgttc gagacgctga cgcttgcgcc gatcgaccgc cgcctgatcg aggttgccat
     1381 gctgagccct gcggaacgcg actggctcga tgcgtaccac gcacgggtac gcatcgaggt
     1441 tcgctcgcat ctcgacgaga ccgacaagct ctggctcgac gccgccaccg cgccgctcac
     1501 atagcgctcg accctgacga cataacagtc ccaaaatcgg catagccgca tgccgaaacg
     1561 accctgttcg cgcagggcga tcccgctaca gtcggttttc agcaccactg aattcgaaac
     1621 ggacatcgca acgcatgcat ggagtgatgg gcaaactgcc gccgacggcc acgaaaaacg
     1681 tcgcggcgtc caagctcacg ctgttgctgc tggtcgcgat gaccggcgtc gcgccgattt
     1741 cgctctacat gctggtgcct gcgctgccca aactcgccac cgctttcggc cgcgacatct
     1801 cgctcacgca gatgacggtg tcgctctaca tggttggtat cgcctgttcg cagctcgtca
     1861 tggggccgtt gtcggaccgc ttcgggcgac gcccggtgct gctttcgggt ctcgccctga
     1921 tggtcgtggc gagcgcagcc tgcatcttcg cgcaaaacct gccgcaactg attatcgcgc
     1981 gatttttgca ggcgctcggc ggcgcaagcg gcatggtgat cagccgcgcc atcatccgcg
     2041 acctctatga gcgcgatcag atcggcgcca agatcagcct ggtgatcgcc gtcatgatga
     2101 tcgcgcagat gctttcgccc ctgaccggcg gcctggtcga gaccgcgttc ggctggcgtg
     2161 cgatcttcta cctcatcacc gccgcagtgc tcgtcaccgt catcgccatc gtgctgctgc
     2221 tgccggagac gcggcgcggc cgcctgcccg gcaacggttt ccgcggcgat ttcggcggcc
     2281 ttttcaagag ccgcgccttc gtcggattca tgctgtgcca ggtactggcg tcgcagatca
     2341 tcttcgcctt tgccggcggc ggaccctaca tcgtggtgac gcagatgagc cgaaccagcg
     2401 ccgaatacgg tgcctggttt gcgaccacgg gatttgccta tctgatcggc aatctcttct
     2461 gcgtgcgctt tgcgccgcgc cattcgctgg aaagactgat ctggttcggg ctcgcgctgc
     2521 aatttgtcgg cagcgcgctc aatctggcgt ggagcatcac tggtatcaac caggcgccgt
     2581 cattcctgtt cggcacgcag atgatcgtaa tgttcgccaa cgccttcgtg atgtcgaact
     2641 cggcggccgg cgcgatcagc gtccgtcccg acgccgccgg caccgcatcg ggcgcgatgg
     2701 gtttcttgca gatgggtatc ggctcgctgg tctcgcaatt cggggcctat ctcggcgggc
     2761 attttgccac gaccctgccg cttaccgcct tcatcttcgc gctgtcgctc gcctgcgcct
     2821 cgaccatgat cttcctggtg ccgcggcgcg cggtcatcgt cagcgaagag ctgatggaaa
     2881 aagccgagga ggaagaatcg gggatgttgt gagggctctg cacctctccc cgcgcttgcg
     2941 ggaaaagggg agctcacttg tcactccccg accatcttca gccattcgtc ttcggtcagc
     3001 acggcgacgc cgtgcttcct ggcctccgcg agcttcgacc cagcgccggg acctgcgacc
     3061 aggtaatccg tcttcttcga caccgagccc gccgctttcg cgcccaggcg ttcggccatc
     3121 gccttggcct cgtcgcgcgt catcttctcg agcgaacccg tgaacaccac ggtcttgccc
     3181 gatatcgccg aactgctcct gggcctttcg gcatcgagga tggtgacctc ctcc
//
LOCUS       DATLJT010000580        14348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1413150,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000580 DATLJT010000000
VERSION     DATLJT010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413150"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            797..1072
                     /locus_tag="VM910_13420"
     CDS             797..1072
                     /locus_tag="VM910_13420"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HVI63568.1"
                     /translation="MNRFLKLAAAPATALFALTFVTLTTSAPAQAGEYCRKDVTSAVV
                     SCGFDTMEQCQVMSSGRGGDCYLDPFLPDRNAFAYAPRAKVRSVKAH"
     gene            complement(1143..1820)
                     /locus_tag="VM910_13425"
     CDS             complement(1143..1820)
                     /locus_tag="VM910_13425"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI63569.1"
                     /translation="MRSLYLKAFGGLAVLFLIMASLLFVAAGTLHYWQAWLFLAVYFL
                     ASLAITLYLINNNRALLARRMSGGPFAEKEPAQRIIMALVSIGFVGLILLPAIDHRFG
                     WSHLSIPAILAGDALVLLGWLGIFFVFRENSFASATIESAEDQRVISTGLYAWVRHPM
                     YATSLLMLLGIPVALGSWWGVLIVVAIVPALIWRLTDEERFLVRHLPDYSTYQRRVRY
                     RLLPRVW"
     gene            complement(1881..2933)
                     /locus_tag="VM910_13430"
     CDS             complement(1881..2933)
                     /locus_tag="VM910_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63570.1"
                     /translation="MRNVAFAAALFALIPSAQAAVAPEIDPRASLSVVQQWIYNYRAK
                     PDYAHVPAAVRVLFHSQTFKEPENAGIYLGFIAGAIGSNPAKAEQLVNSFFPVPPEDE
                     WVIVRAIAYSGLPDWRNLLRRVAPKMPGRRVMIDAYLSSTLPILTEIPLEEKKPGMMD
                     KLRGVFTKNPFAKDEHKLNTTVTFAANQDLLDTLWGYYFATGSHLPIMRIMQMLPWSK
                     SRDTVDKLTVGSMARYTLASYAVRDTELREFLRSELPKQPEPIKLPLTEVIQAADTVQ
                     LGAVRKDAIAAVDELKSKGSDSRRNLDFWGQVGVGAVALGCVSAAAMGAVAVGIPCVI
                     GGSASQGLLSFWEKQQ"
     gene            3246..5516
                     /gene="bamA"
                     /locus_tag="VM910_13435"
     CDS             3246..5516
                     /gene="bamA"
                     /locus_tag="VM910_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HVI63571.1"
                     /translation="MLVLPVMPAASSDVRASISVEGNRRVDADTVRSYFPPSPDGRYD
                     EAARDAALKALIATGLFDKVSIERAGDHLVVRLVEAKVLNRVAFEGNKKIKDADLAAA
                     VQSKAQGSLQRATVQADVGRIVEAYRRVGRDDVSVVPEIIDRGNDRVDLVYAITEGKK
                     TTVRQIIFVGNHAFADRQLKAVIKTSATSVISFLTGGDVYDRDRVDEDREQIRLYYRG
                     HGYADASVPSARAEYDPATRGFALTFTIDEGQLYNFGEIGVASNVPGLDPRGFRRLLL
                     VKTGAQFDGNALDKSSEALAIEMAKLGYPFARAVPRTVRNAEARRIDVTFVIDEGKRT
                     YIERIDIHGNTRTRDYVIRREFDFGEGDAYNQTLIDRAERRLKSLNYFKTVKISTKPG
                     STSDRVVLDVEAVDQSTGDFWISGGYSTTDGFLVEVKVGDRNFYGTGDSVQATVSYGQ
                     YGRGGDISVTDPDLFGNHVSGGVQVFDRQTFASPYQSYGTNTYGANFTVGTPITEQTT
                     VLWRYTLYDQDVTLSPTSGAVVSLPIQQAAAAGRQLVSAAGDTITYNTLDNTKSPTSG
                     VNSQLKQDIAGLGGDVKFLRTTEDVRYYQSLNSDLVAMVRGQGGYVTGWGGQQVPLMN
                     SFFGGPSLVRGFAPNGFGPRDLTPSSTMDNVGGSMYWATTAELQSAIPGIPQEYGLRG
                     SVFVDAGSVFRYTGPTTFGTQSVQVANNNVMRSSVGVGLTWASPFGALTINYAEPLTK
                     ASYDVVQRFNFTTGPF"
     gene            5658..5942
                     /locus_tag="VM910_13440"
     CDS             5658..5942
                     /locus_tag="VM910_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HVI63572.1"
                     /translation="MKKWRNESALDVYNLVAAAVLLAAPWLFSLTNGSARLDFRLSAA
                     AIVAMSLAAMFAYANWEEWINTLVGLWLIVSPWVLGFAYVRAMHFSEASW"
     gene            complement(6032..6630)
                     /locus_tag="VM910_13445"
                     /pseudo
     CDS             complement(6032..6630)
                     /locus_tag="VM910_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973850.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6676..7206)
                     /locus_tag="VM910_13450"
     CDS             complement(6676..7206)
                     /locus_tag="VM910_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HVI63573.1"
                     /translation="MESFNWPAEHSEALRQYMAQGMSYSAAADAINKKFHTAYTRNAA
                     ISRGKRMGLTSANRPHEPPMRLSRTVKPGASRLRSWRADELALPWPPCKPAKRVKLRC
                     VGVKPRLLALVDLEAGDCRYPYGGDKDGEAITFCGHPCLPGVSYCAAHFHLTRGDGTA
                     SERAAAPIVLRLVAVA"
     gene            7360..7992
                     /locus_tag="VM910_13455"
     CDS             7360..7992
                     /locus_tag="VM910_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVI63574.1"
                     /translation="MLDAAMIVRALKRTGKSKGGLAQAMGVRPGAVSEILSGIRLIKA
                     SEIAPITEYLGLNSVPIMGRVGAGAVIEPENEQIPPEGLGEVELPFPISEETIAFEVA
                     GDSMLPKYENGDVIVVYREQRHPLASFYGEEAAVRLKTGERYLKTIERGKSANMVNLT
                     SFNAKPINGVKLEWIGEICMTLPRGQIGRLRAKTAGRARKARHGSRQTEK"
     gene            8131..8405
                     /locus_tag="VM910_13460"
                     /pseudo
     CDS             8131..8405
                     /locus_tag="VM910_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8482..9456)
                     /locus_tag="VM910_13465"
     CDS             complement(8482..9456)
                     /locus_tag="VM910_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HVI63575.1"
                     /translation="MTHAIRFHKPGGPDVLVWEEVNVGKPGPGQARIGHTAVGLNFVD
                     IYNRSGLYPLQLPSGLGGEAAGVVEEVGPGVTDLKPGDRVAYGAAPIGAYAEARLIPA
                     DRLLKLPDSIDDKTAAAMMLKGLTTQYLIRQTYRVKRGDTILLHAAAGGVGLILSQWA
                     KHLGATVIGTVGSDEKAKLAKAHGCAHTIVYTREDFVKRVDEITEGKKVPVVYDSVGK
                     DTFLKSLDCLAPLGVAALFGQSSGSVEPLNLGLLAQKGSLYVTRPTLNTYAAKHENLV
                     AMAKELFEVVQSGAVKIEVRQTYPLKDAAKAHADLGARKTTGSTVLLV"
     gene            complement(9525..11753)
                     /locus_tag="VM910_13470"
     CDS             complement(9525..11753)
                     /locus_tag="VM910_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI63576.1"
                     /translation="MNAPVILDRRHVLKGGGALIISFSLTRAFAQDQTVPAAAPPSPP
                     GSLKNSPYLDSWIRIDADGVITVFTGKAELGQGFKTAFQQIAAEELNVPFASLKVVTA
                     DTKLTANEGYTSGSHSMQDSGTAIQNAAAQARELLLAEAAKRLDVSIDTLQSEDGVVI
                     APDGRRLSYGELVSGDMLHVQAQPSSKLKDPSTLKVMGQSIPRVDIPAKVTGGVAYVQ
                     DLRLSGMVHARVVRPPSYGAQLVDCDVAAIEKMPGVVKVVRDGNFLAVVAAKEFQAIK
                     AMNALANAAKWQEVAQLPKLNDLPSVLTNLASQDSVIFQRSDPSVSGQRTIEATYTRP
                     YQSHGSVGPSCAVAQLADGAMTVWSHTQGVYPDRQAIADMLRMPSASIRVIHVEGSGC
                     YGHNGADDAAADAAMIARALPGVPVRVQWTREQEHAWEPYGPAMVTRLKASLDAAGKI
                     ADWNFEVFSNTHSMRPGGAGCLLAAQHMAQPVAVPPAKPIPLPEGGGDRNAIPIYTFP
                     NAHVVHHFIPTMPIRISAMRALGAYHNVFSIESFMDELAALAGVDPVEFRLNHLDDPR
                     GRDVIEKAAQGFGWRKGQAASDRGFGFAFARYKNLAAYCAIASEVEVNRETGRARLVR
                     AVAAVDSGQVVNPDGLINQIEGAIIQSTSWTLYESVTFDATRITSIDWQTYPILRFDA
                     VPESIEVHIINRPGKPFLGSGETGQGPAAASVANAIANATGIRLRDLPLTRKRIKDAI
                     DS"
     gene            complement(11750..12247)
                     /locus_tag="VM910_13475"
     CDS             complement(11750..12247)
                     /locus_tag="VM910_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI63577.1"
                     /translation="MTTLKVNGREHQVDAEPDTPLLYVLRDDLKLNAAKFGCGLGQCG
                     ACTVIVDGKAVLSCVTPLVLLEGKQVTTLEGLGTISEPAPIQRAFMEEQAAQCGYCIA
                     GMMMRAQALLLRIPKPTDQQIRAELAPNLCRCGTHMRILRAVHRAARLMETAALSPAE
                     KRSDK"
     gene            complement(12393..12791)
                     /locus_tag="VM910_13480"
                     /pseudo
     CDS             complement(12393..12791)
                     /locus_tag="VM910_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
     gene            12882..13409
                     /locus_tag="VM910_13485"
     CDS             12882..13409
                     /locus_tag="VM910_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63578.1"
                     /translation="MRVDRVSLPVIDIAAGKCRAGALRAQRITRRMARAAMRETLDQI
                     GAAIPFRALRRIGPIGPAMKKQQLPARDHKALIEWKGKLVLALWCTNGLACHQIGVER
                     VVVLIADMREVIVGECRIEMPAFPIDAHAHGTAEGLFRPPPYAGLRIRRDVGRIDGAE
                     RRRHWNAAREVLSTH"
     gene            13785..13958
                     /locus_tag="VM910_13490"
     CDS             13785..13958
                     /locus_tag="VM910_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63579.1"
                     /translation="MVREDPARPPPQAEQEPYPAEKARGGEIILDTPLKRWIFFGGLV
                     GAVLLALALSFLR"
BASE COUNT         2734 a         4471 c         4424 g         2719 t
ORIGIN      
        1 ctcatcatcc tcccgtacat atagcaaggt taccatactt gccgggcggc gtctttgcga
       61 tggcgcaaca caactttttc gacccgggga tcgcccgaag ccgggatctt gcagccatcc
      121 cgggccacgg acgccgcgcg atcagttgaa ctagccttcc gaccggcctg cttcaccggc
      181 agttcgcgat ccgcccgccg cgcaggtgca tacacagccg caggaaagac cggaacaagc
      241 accgacaccg cgagcacgac atgggtctca tcgacccctc catcagtgcg ctgattggtc
      301 ggcaggggtg actccggcgt tcggcagcgg ccggttacca ccggaaagtc tggctgcgaa
      361 ggacggcgtg aacgttcaca tttttgtgca tctggcgcga gcgatgtcga cgcgtcgggc
      421 gcgcgacgcc tccgacgctc ctcgctgtcg cggcgcgcaa cgattgctcg atcgagcccg
      481 gcacagtggc catctcctca agagatgacg accatacttc aaatgcgcca cagcacggtg
      541 aattttcact ctctacgagt ccaagcggga gaattgcatt tcacggcatc acaacagcga
      601 gactgcgatt gattttgtgc cgataaatcc gggtgcttct tctcttcgga tcaaacgcgg
      661 caactctgat tgcgagcagc ttcttaccaa taacaggttt gagagcgcat cagccatgcg
      721 cgcgtagcgt cgcacgtgac gacatcccac gtgcgaaccg aggctgtaaa gccaccacgt
      781 aaagagagag agcaccatga accggttttt gaagctggcc gcagcgccgg caacggcgtt
      841 gttcgccctg actttcgtta ctctgacaac ttctgcgccg gcgcaggccg gcgagtattg
      901 ccgcaaggac gtcacgtccg ccgtcgtatc ctgcggcttt gacacgatgg agcagtgcca
      961 ggtgatgtcg tccgggcgcg gcggcgactg ctaccttgac cccttccttc ccgacagaaa
     1021 cgcgttcgcc tacgcgccga gagcgaaggt gaggtctgta aaggctcact gaagcgtggc
     1081 ccatccgctt caaatcccgg ccgcggcctt gcgatcactt gcaaggccgc gcgctttcga
     1141 agtcaccaaa cccgcggcag cagccgataa cggacccggc gctgatacgt cgaatagtcc
     1201 gggagatgcc tcaccaggaa gcgctcctcg tccgtgagcc gccagatcag cgccggcacg
     1261 atcgcgacca cgatgagcac accccaccag gacccaagcg cgacgggtat gccgagcagc
     1321 atgagcaacg acgtcgcata catgggatgg cggacccacg cgtagagacc ggtggagatc
     1381 accctttgat cttcggcgga ctcgatcgtc gccgaagcaa agctgttttc ccgaaagacg
     1441 aagaaaattc cgagccagcc gagcaagacg agcgcgtcgc ctgcaaggat cgcagggatg
     1501 gacaggtgcg accagccgaa gcgatggtcg atggcgggca gcaaaatcag gccgacaaag
     1561 ccaatcgaga cgagcgccat gatgatccgc tgtgccggct ccttctccgc aaacggtccg
     1621 ccgctcattc gtcgcgccag cagcgcgcgg ttgttgttga tgaggtacag ggtgatcgcg
     1681 agagatgcga ggaaatagac cgcgaggaat agccaagcct gccagtagtg caacgtgccg
     1741 gcggcaacga acaatagcga cgccatgatc aggaacagga ccgccaatcc gccgaacgcc
     1801 ttgagataca gggagcgcat acaccgaatc cagcacgcgg acacgaccca tcgcgggctt
     1861 cacttcgttt ttgcgggggc ttactgctgc ttttcccaga acgacagcag cccttgcgat
     1921 gccgagccgc cgattacgca gggaatgccg accgcaaccg ctcccatcgc ggccgccgag
     1981 acgcagccca gcgccaccgc accgacgccg acctgacccc agaagtcgag attgcggcgt
     2041 gagtccgatc ccttggactt gagctcgtcg acggctgcga tggcatcctt acgtacggcg
     2101 ccgagttgca cggtgtccgc agcctggatc acctcggtga gcggaagctt gatcggctcc
     2161 ggctgcttcg gcagctcgct acgaaggaat tcccgcagtt cggtatcacg aacggcgtag
     2221 cttgcgagcg tatagcgcgc catgctgccg accgtcagct tgtcgacggt gtcgcggctc
     2281 ttcgaccatg gcagcatctg catgatgcgc atgatcggca ggtgcgagcc ggtggcaaaa
     2341 taatatcccc aaagagtgtc cagaaggtcc tgattggcgg cgaaggtcac cgttgtgttg
     2401 agcttgtgct cgtccttggc gaacggattc ttggtgaaca cgccacgcag cttgtccatc
     2461 atgcccggct ttttctcctc gagcggaatc tcggtgagga tcggcagcgt gctggacaga
     2521 taggcatcga tcattacccg ccttcccggc attttcggcg cgacccgccg cagcaggttg
     2581 cgccagtccg gcagacccga ataggcgatc gcacggacga tgacccattc gtcctccggc
     2641 ggcaccggga agaagctgtt gacgagctgt tcggccttcg ccgggttgga gccgatcgca
     2701 ccggcgatga agccgagata gatgccggcg ttctccggct ccttgaaggt ctgcgagtgg
     2761 aagaggacgc gcaccgcggc cggcacatgg gcatagtcgg gcttggcgcg gtaattgtag
     2821 atccattgct gaaccacgct gagcgaggcg cgtggatcga tctccggggc caccgcagcc
     2881 tgcgccgacg ggatcagcgc gaacagggcg gcggcaaacg caacgttacg catcagatac
     2941 tcccacgagg tgtcccattg ttgctttcgg gcaccacacg gacctttcgc gctgccttgg
     3001 ctaagcggca tttggagcgg ctatgcgaaa gattcaggga acgggcggtt cagtatggcg
     3061 tcgaaattgt ggcggccttg acgattggcc cgcaactcac cttgggaccg acattctttc
     3121 gccctcggca ggcaattcga tttgggcctg ccaagcgagg aaatgggcag gacgtgacga
     3181 ccatatgcca ggccctgggc cggcacgccc gccgtctgca aatcgcgggc gtgctgcttg
     3241 ctgccatgct cgtgctgcca gttatgccgg ccgcctcgtc cgacgtgcgc gccagcatct
     3301 ccgtcgaggg caaccggcgc gtggacgccg ataccgtgcg ctcgtatttt cccccctcgc
     3361 cggatgggcg ctatgatgag gccgcccgtg acgcggcgct gaaggcgctg atcgcaactg
     3421 gcctgttcga taaggtatcg atcgagcgtg ccggcgatca tttggtcgtg cggctggtcg
     3481 aagcgaaagt gctcaatcgc gtcgccttcg aaggcaacaa gaaaatcaag gacgccgatc
     3541 ttgccgctgc cgtgcaatcc aaggcgcagg gctcgctgca gcgcgccacg gtgcaggccg
     3601 atgtcgggcg catcgtcgaa gcctatcgcc gcgtcggacg cgacgatgtc agcgtggtgc
     3661 cagagatcat cgatcgcggc aacgaccgcg tcgatcttgt ctacgccatt accgaaggca
     3721 agaagacaac ggttcggcaa atcattttcg tcggcaatca cgccttcgcc gatcgccagc
     3781 tcaaagccgt gatcaagacc tcggctacct ctgtcatcag ctttctgacc ggcggtgacg
     3841 tctatgaccg agatcgcgtt gacgaggacc gcgagcagat ccggctttac tatcgcggcc
     3901 atggctatgc tgatgccagc gtgcccagcg cgcgtgccga gtacgaccct gcgacaaggg
     3961 gatttgcact gactttcacg atcgacgagg gacaactcta caattttggc gagattggcg
     4021 tagccagcaa cgtgccgggg ctcgacccgc gaggattccg ccgtctgctt ctggtgaaga
     4081 ccggcgcgca attcgacggc aacgcactgg acaagagcag cgaggcgctc gccatcgaaa
     4141 tggcgaagct cggctatccc ttcgcgcgtg ccgtgccgcg aaccgtacga aacgccgagg
     4201 cacggcgcat cgacgtaacc ttcgtgatcg acgagggcaa gcgcacgtat atcgagcgca
     4261 tcgatattca cggcaacacg cgcacccgcg actacgtgat ccggcgcgag ttcgacttcg
     4321 gcgaaggtga cgcctacaac cagaccctga tcgaccgtgc cgagcggcgc ctgaagagcc
     4381 tgaactattt caagaccgtc aagatctcga ccaagccggg ctcgacgtcg gatcgcgtgg
     4441 tgctcgatgt cgaggccgtc gatcaatcga ccggcgactt ctggatctcc ggcggttact
     4501 cgaccaccga cggatttctg gtcgaggtca aggttggcga tcgcaatttc tacggcaccg
     4561 gagacagcgt gcaggccacg gtaagttacg ggcaatatgg gcgcggcggc gacatatcgg
     4621 tgaccgatcc cgacctgttc ggaaaccatg tttccggcgg cgtgcaggtg ttcgacaggc
     4681 agacattcgc gagcccctac cagtcctacg gtaccaacac ctacggcgcg aatttcacgg
     4741 tcggcacgcc gatcaccgag cagaccaccg tgctgtggcg gtatacgctc tatgaccagg
     4801 acgtcacgct cagcccaact tcaggtgcgg tcgtgtcgct gccgatccag caggcggcag
     4861 cggccggccg gcagctcgtc tccgcagcgg gcgataccat cacctacaac acgctcgaca
     4921 acaccaaaag cccgaccagc ggcgtcaatt cccagctcaa gcaggatatc gccggacttg
     4981 gcggcgacgt aaagttcctg aggaccacag aagacgttcg ctattaccag tcgctcaaca
     5041 gcgacctggt ggcgatggtg cgcgggcaag gcggctacgt aaccggctgg ggcggccagc
     5101 aggtgccgct gatgaacagc ttcttcggag gtccgagcct ggtgcgcggt tttgcgccca
     5161 acggctttgg cccgcgtgac ctcacgccga gctcgacgat ggataacgtc ggcggcagca
     5221 tgtattgggc cacaaccgcg gagcttcaaa gcgcaatccc cggcattccc caggaatacg
     5281 gactgcgtgg atcggtcttc gtcgatgccg gcagcgtgtt tcgctacacc ggtccgacca
     5341 ccttcggcac gcagtccgtg caagtcgcga acaataacgt catgcgctca tccgtgggcg
     5401 ttggcctgac ctgggcctcg cccttcggcg cgctgacgat caactacgcc gaaccgctga
     5461 ccaaagcgtc ctacgacgtg gtgcagcgat tcaatttcac caccggaccg ttctagatcg
     5521 accgcgcgac ggcccggcca gcgcggccgg cacgcgctac gacaaaagtc tagcatttcg
     5581 ggattgggct atccggaacc gcgggggaac caaggccgtt tctctcttgc atcagatcct
     5641 cacggggaga tcgaagcatg aagaaatggc gaaatgaatc tgccctcgac gtctacaacc
     5701 tcgttgccgc ggccgtcctg cttgcagccc cgtggctgtt cagcctcacc aacggatcgg
     5761 caaggttgga cttccggcta agcgcggccg caatcgtcgc gatgtcgctg gcggccatgt
     5821 ttgcctatgc gaactgggaa gaatggatca atactctggt gggcctgtgg ctcatcgtct
     5881 cgccctgggt gctcggattc gcgtatgttc gcgccatgca tttcagcgag gcatcgtggt
     5941 gaccttcatg gctttgctgg agctttggct ggtctatgac cgtcattccc cgcatcaggt
     6001 accgacgccg acgtcgggac agagctgagg gctagcgctc ctcaggtgga tgctcgcgcc
     6061 tgatcgtcgc cctgtcgcca ggccgcctct cggttgcaaa gccgtacagc aacgccaggc
     6121 gatccaggca ctcgcgaacc tgcgcgagaa ataatcgttc cagcgcgcac tgcgcaggct
     6181 gcggcgctgt ccgacctgcg ccatggtcat gcccttgatc aggacatcat ggatcagcgc
     6241 cgatccatcg gcacccagct cccgctcggc gcggttgagc cgcaacacta ccctgcgctg
     6301 accctcggtg attggctcgc gggtccgcac gccatcgaca tattcgcggg tgggatcgac
     6361 ggcctgcggt ccgcgttcgg cccgttccca atcgttctga aacgcgcgac cgcttcgata
     6421 ttgggcttcg tcgatctgcc ggtgcgaatg cagccgcgcc agcggatcgt tgcggatcga
     6481 gcgcaaggcg acgatcttgt cgccgggttc cagcgccatg ggatcgtcga cctcaatggg
     6541 agcgacttcc gcgttgtgcg gcagatcgcg ggaacgccga tcgtgaatct ttgacagacg
     6601 gtatgacttc ttgcgcctgg cccgtgccac ggtgccccct ttcgcatgag attggaaatg
     6661 atttgcctgc ggcggtcagg ccacggcgac cagccgcaac acgatcggcg ccgccgcgcg
     6721 ttccgaagcg gtgccgtcgc cgcgcgtcag atgaaaatgc gctgcgcaat agctgacgcc
     6781 cggaaggcac ggatggccgc agaaggtgat ggcctcgcca tccttgtcgc cgccatacgg
     6841 atagcggcaa tcgccggctt cgagatcgac caacgcgagc agccgcggct tgacgccgac
     6901 gcaacgaagc ttgacccgtt tggcaggctt gcaaggcggc cacggcaaag ccaattcgtc
     6961 ggcgcgccaa cttcgcaatc gcgatgcgcc cggctttacg gttcgggaaa gtctcattgg
     7021 aggttcgtgc gggcgattgg cgcttgtgag ccccattcgc ttgccccggc tgatcgcggc
     7081 attccgggta taggctgtat gaaatttctt gttgatggcg tcggctgctg ccgaatagga
     7141 catgccctgc gccatatatt gccggagcgc ttcggagtgc tccgccggcc agttgaacga
     7201 ttccatggtc tgccttttct tggcgttctg gcgcctcagc cggcggcgcg aggtcgcatc
     7261 gacggcaaat cacattccga tattcagaaa tgaaagtcaa gcttgattta tgataatcgt
     7321 aatcttagga tgattacggg aatcggaagg aggatcgtga tgcttgatgc tgcaatgatc
     7381 gtgcgggcgc tcaagagaac cggcaagtcg aaggggggtc ttgcgcaggc gatgggcgtg
     7441 cgtcccggcg ccgtatcgga aattctgtcg ggaatccggc tgatcaaggc ctccgagatc
     7501 gcgccaatca ccgaatatct cggattgaat tcggtgccga tcatgggacg tgtcggcgca
     7561 ggcgcggtga tcgagccgga gaacgagcag attcctcccg aaggcctggg tgaggtcgaa
     7621 ctgcccttcc cgatctccga ggaaaccatc gccttcgagg tcgccggcga ttccatgctg
     7681 ccgaaatacg agaatggcga cgttatcgtg gtctatcgcg aacagcgcca tccgctcgca
     7741 agtttctatg gcgaggaggc tgcggtgcgc ctgaagaccg gcgagcgcta tctgaagacg
     7801 atcgaacgcg ggaagtccgc caatatggtc aacctcacga gtttcaacgc caagccgatc
     7861 aacggcgtga aactggaatg gatcggcgag atctgcatga ccttgcccag gggccagatc
     7921 gggcggttgc gggcgaagac ggccgggcga gcgcgcaagg ccaggcacgg cagccgtcag
     7981 acggagaaat gagacggccg ccgaccggga agccgcttca tcttccatat ttctgttttt
     8041 caaaattaaa tcttgacttg tttccgattt tcggaaacaa tgccgccttg tttgattctt
     8101 tcgccgggaa caaggagatc atctccccgc atgcagtatt ttgtcgtcat gatcgactat
     8161 ggccgccgcg gtcgcgaggc tgtggtcgat ccggaaataa cccgacgcga agtggtctcg
     8221 cgcgtggcgt cgggtgaata caagaacatc agcttcattc acgaaaagcc gatcggtccg
     8281 tcgaggatgt gaccgaggag atcctggccg aggcggtgct tccggaaatt tcggcggaca
     8341 gtgccgatct gcaggccgcc cgctttgatc atgcgcgcga cctgcgcaag catgagcgcg
     8401 cgtgacgacg cgatgccgac cggcccgggg tagccccgga gcgatcggcg gcgtctcgac
     8461 cctccgttcc gatccggcag ttcagaccaa cagcacggtc gatcccgtgg tcttgcgcgc
     8521 gccaagatcg gcatgtgcct tcgctgcgtc tttcagcgga taggtctgac gcacctctat
     8581 cttgaccgcg cccgactgca ccacctcgaa cagctccttg gccatcgcga ccaggttttc
     8641 atgcttggcg gcataggtat tgagcgttgg acgggtcacg tacaacgatc ccttctgcgc
     8701 aagcagacca aggttgagcg gctcgacgct gcccgaggac tggccgaaca gcgcagcgac
     8761 gccgagcggc gcaaggcagt cgagtgattt caggaaggtg tccttgccga cggagtcata
     8821 gaccaccggc accttctttc cttctgtgat ctcgtccacg cgcttcacga aatcttcgcg
     8881 ggtgtagacg atcgtgtgcg cgcagccatg ggctttcgcc agcttcgcct tctcgtcgct
     8941 gccgacggtg ccgatcacgg ttgcgcccag atgcttcgcc cattggctga ggatcagccc
     9001 gacgccgccg gccgcagcat gcagcagaat ggtatcgccc cgcttgacgc gataagtctg
     9061 gcggatcaga tattgcgtcg taagaccctt caacatcatc gccgccgcgg tcttgtcgtc
     9121 gatgctgtca ggtaatttga gcaggcggtc ggcggggatc agccgcgctt cggcataggc
     9181 gccgatcggc gcggctccat aggcgacacg gtcgcctggc ttcaggtcag tgacaccggg
     9241 gccgacttcc tcgacgacgc ccgcggcctc gccgccaagc ccgctcggca actgcaaagg
     9301 atagagcccc gagcgattgt agatgtcgac aaaattcagg ccgaccgcgg tgtggccaat
     9361 gcgagcctga ccggggccgg gcttgccgac attgacctct tcccagacca gaacatccgg
     9421 accgccgggt ttgtgaaagc gaatggcatg ggtcatggcg ttactccctt gatttgatgt
     9481 tgcatgatgg tcgaatggct atgtttccgt aacggcctgc tgcttcacga gtcgatcgcg
     9541 tccttgatgc gcttgcgcgt gaggggcagg tctcgcagcc gaatgcccgt ggcattggct
     9601 attgcattgg cgaccgaggc ggcagccggc ccctgccccg tctcgccgct gccaaggaag
     9661 ggctttccgg ggcggttgat gatatggacc tcgatcgatt cgggtaccgc atcgaagcgc
     9721 aggatcggat aggtctgcca gtcgatgctg gtgatccggg tcgcgtcgaa ggtcacactc
     9781 tcatagagcg tccagcttgt cgactggatg atcgcgcctt cgatctggtt gatcagcccg
     9841 tccggattga ccacctgacc actgtcgacg gcagcgaccg cacgcaccag ccgtgcgcgg
     9901 ccggtttcgc ggttcacttc gacctcgctc gcaatcgcac aataggctgc gagattcttg
     9961 tagcgtgcga atgcaaaacc gaagccgcgg tcagacgccg cctgcccttt gcgccagcca
    10021 aagccctgcg cggccttctc gatcacatcg cggccgcgtg gatcgtcgag atggttcaat
    10081 ctgaattcga ccggatcgac tcccgcaagc gctgcgagct catccatgaa actctcgatc
    10141 gaaaatacat tgtggtaagc accgagcgcc cgcatcgcgg aaatccggat cggcatcgtc
    10201 gggataaaat gatgcacgac atgcgcgttt ggaaacgtgt agatcgggat cgcattgcga
    10261 tcaccgccgc cttccggaag cgggatgggt ttggcaggcg gtaccgcaac gggctgcgcc
    10321 atatgctggg cagccaacag gcagccggcg ccacccggcc gcatcgaatg cgtgttgctg
    10381 aaaacctcga aattccagtc ggcaattttg cccgcggcat cgagtgaggc cttcagcctg
    10441 gtgaccatgg ccggcccgta aggctcccag gcgtgctctt gctcgcgcgt ccattgcacg
    10501 cggaccggca cgcccggcaa tgcacgggcg atcattgcgg catccgccgc agcatcgtcg
    10561 gcgccgttgt ggccatagca accggaaccc tcgacatgga tcacgcggat actcgcagac
    10621 ggcatccgta gcatgtctgc aatggcctga cggtccggat agaccccttg cgtgtgcgac
    10681 cacactgtca tcgccccatc cgcaagttgg gcaaccgcgc aggatggccc gaccgagccg
    10741 tgcgattgat aggggcgcgt atatgtggct tcgatggtcc gctggccgga cacggatgga
    10801 tcgctgcgct gaaaaatgac cgaatcctgc gacgccaagt tcgtcagcac cgatggcaga
    10861 tcgttcagtt tcggcagttg cgcgacctcc tgccatttcg cggcgttcgc aagagcgttc
    10921 atcgccttga ttgcctggaa ctccttggcc gcgaccacag caagaaaatt cccgtcacga
    10981 accactttca cgacgcccgg cattttttcg atcgcggcaa cgtcgcagtc gacgagttgt
    11041 gctccatagc ttggcgggcg caccacgcgt gcatgcacca tgccggagag ccgcaagtcc
    11101 tgtacatagg cgaccccgcc cgtcactttc gcgggaatgt ccacgcgcgg gatcgattgc
    11161 cccattacct tgagcgtcga tgggtccttc agcttcgatg agggttgtgc ctgcacgtgc
    11221 agcatgtcgc ccgataccag ttcgccgtaa ctcaagcgcc gtccatcggg cgcgatcaca
    11281 actccgtcct cggactgcaa agtgtcgatc gagacgtcga gccgcttggc ggcttcggcg
    11341 agcaaaagct cgcgcgcctg cgccgcagca ttctggattg ccgtgccgct gtcctgcatc
    11401 gaatggctgc cggaggtgta gccctcattg gcagtgagct tggtatcggc ggtcaccacc
    11461 ttcagcgagg cgaagggcac attgagctct tcggccgcga tctgctggaa ggcagtcttg
    11521 aatccttgcc cgagttcggc cttgccggtg aaaacggtga tcacgccgtc ggcatcgatg
    11581 cgaatccagg aatcgagata gggtgagttc ttcaggctcc ccggcggact cggaggcgct
    11641 gcggcgggga ccgtctgatc ttgcgcgaaa gcgcgcgtca aagagaagct aatgatcagc
    11701 gcgccgccgc ctttcagcac gtgacggcga tcgaggatga ccggcgcgtt catttgtcgc
    11761 tcctcttctc tgccggcgac agcgcggccg tttccatcag ccgtgcagca cggtgcaccg
    11821 cccgcaagat gcgcatgtgg gtaccgcagc ggcacaggtt cggcgcgagc tcggcgcgga
    11881 tctgctggtc ggtgggcttg gggatccgca gcagcagcgc ctgcgcccgc atcatcatcc
    11941 cggctatgca atagccgcat tgcgcggcct gctcctccat gaaggcacgc tggatcggcg
    12001 ccggttccga gatcgtcccc aggccttcca gagtggtcac ctgtttgcct tcgagcagca
    12061 ccagcggcgt tacgcaggac agcacggcct tgccgtcgac gatgaccgta caggcgccgc
    12121 attgcccgag tccgcagccg aatttggcgg cgttcagctt gagatcgtcg cgcagcacat
    12181 agagcagcgg ggtatccggc tctgcgtcga cctgatgctc ccgaccattg accttcaatg
    12241 tcgtcatcgc ttgtctcgct gcgcttgatc gggggaggca gggcgcgcgc cggccccgtg
    12301 ttcaaagatg cggtctgggt acgccgcgcc tccgcgatcg tcctttcgat gtcgctccag
    12361 gccggctgat cgctgaagcg cgatcgcaga tagtgtaata acaccgcgat ctgcgcatca
    12421 ttcatggtgc tggcaaaacc tggcatgatc gggctgctct cgccctcgac tggcgccaca
    12481 ccggatagca cgatattggc aagattgcgc ggatcagaac cgctgattgc ggtgctgagc
    12541 cccagattga tgcctccata aggcggcggc ctgccgcttt cgtggcaggt cgcgcaggct
    12601 gcagcataga tcgccgcgcc cggtgaggcc ggagtttcca ctgcgggagt cgattttgcc
    12661 tgtgcaagtg ccgcctcgcc cctgaccttg cggctctgcg ccggctggcc cgacatgtcg
    12721 gccacatagg tcgcaatcgc ccgcacatcg ttcgccgaaa ccgccgacag attgctcacc
    12781 accgctgcca tcggtccgcg cgccgtgccg tgatcgggat gccagccgtt gcgcaaatag
    12841 gcgtagagcg cctcggcatc ccaaggcacc ggcgatgggg attgcgcgtt gatcgcgtaa
    12901 gcctgccagt tatcgacatt gccgccggca aatgccgcgc tggcgcgctc cgcgcccagc
    12961 gcattacgcg gcgtatggca cgcgccgcaa tgcgcgagac cctcgaccag ataggcgccg
    13021 cgattccatt ccgcgctctt cgtcgcatcg ggccgatagg tcccgcgatg aagaaacagc
    13081 agcttccagc ccgcgatcac aaagcgctga ttgagtggaa aggaaagctg gttctcgcgc
    13141 tgtggtgcac gaacgggctc gcgtgtcatc aaataggcgt agagcgcgtt gtcgtcctca
    13201 tcgctgacat gcgtgaagtg atcgtagggg aatgtcggat agagatgcct gccttcccga
    13261 tcgacgccca tgcgcatggc acggcggaag gcctcttccg accaccgccc tatgccggtc
    13321 tccggatcag gcgtgatgtt ggacgaatag atggtgccga acggcgtcgg cactggaacg
    13381 ccgcccgcga agtcctttcc acccactgac gtgtgacagg tgctgcaatt gccgatcgcg
    13441 gcaagttccc ggccccgcct gacgcgggcc gcgtcgaagg attgcggctg cggcgggtcg
    13501 attgcggcga acgcaggccg ccatgcaatg acaaaagcgg tcgccgcgcc gcccgcaacg
    13561 agcgcagcca gaatggcccc gattactctc gacctcgtca tccgtcccag cggctttttt
    13621 gaaggttcct agcataatcc cgcgtaagtg ccgagccgct cgatccggtt caccgcctgt
    13681 tgattcagcc ctttcgcgca agccggcgcg ctacgggtgg gtgctccagg aaccccggag
    13741 ggcgcaggac gttccatcgc gatacgataa agaggcccaa gaacatggtc agagaagatc
    13801 cggcacgacc cccgcctcag gccgagcagg aaccctatcc agccgagaag gcgcgcggcg
    13861 gcgagattat tctggacaca ccgctcaaac gctggatttt tttcggcggt cttgttggtg
    13921 cggtgctgct cgcgcttgcg ctcagtttcc tgcgttgaag gacagtcgcg aagtgcattg
    13981 ccggaaacac cgatgaattc cctctgctcc ctcacagagg gatcggtgac ggtatgaccg
    14041 ttacaggaac gcaacatggg cgaacaatat cccgccgttc catgtccgtt cgagcagagt
    14101 taatgacgcg catctcgcat ctgagcgtag aatctttttt ggggtagtga ttcgttttct
    14161 tccttctcgc ggcgaggaga gaaccgaatg cctgcctata cccaccctca cgacatcgag
    14221 atggcgccgg tcatctgccc cggctgcgca gggtcgttgc cgatgtatgt gcgcgacgtc
    14281 gagccgcatt ggaccatggc gaagatcgac ttcatctacg agtgctcgga atgcggcgca
    14341 gaggtgag
//
LOCUS       DATLJT010000581         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_264977,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000581 DATLJT010000000
VERSION     DATLJT010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264977"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VM910_13495"
     CDS             complement(<1..855)
                     /locus_tag="VM910_13495"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HVI63580.1"
                     /translation="MGRVFVAGSINMDVVAIAERHPALGETVAGKEVLYFPGGKGANQ
                     AVAASKLGAATTLIGRLGKDAFGRELRTFLADQGIDLCLVRDTADAHTGTAIITLANA
                     ENTIVVVPGANDLVTPDDVASAVLGKGDIAVSQFEIPLATINAFFRRARAAGASTMLN
                     PAPARALDAELLDLVDILVLNETELALLARVALSGSDDDARFADAARSLRRHDDQIIC
                     VTLGERGLVALVNGKPLIVPGRKVEAVDSTGAGDCFVGALAARLADNQPVQDALGYAN
                     VAASICVQR"
     gene            complement(868..1761)
                     /locus_tag="VM910_13500"
     CDS             complement(868..1761)
                     /locus_tag="VM910_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HVI63581.1"
                     /translation="MQALFIGQTYIDVTFITDHLPTGDEKHVAEAYAVSFGGNAVTAA
                     FCCAKLGIVPDLIATVANDWLGRMFMDMSAKYGISVHPRKVNASSLSFIMPKDGKRAI
                     VRCRDDAHIHPFPLLNLGNCRALHIDGHQPDAAIHYAKLCREEGILTSLDGGGLRTNT
                     HDLLQYIDVAIVAERLCEQMDLTIEKMLDYLKERGCRVGGVTLGEKGLLWYDETGAVH
                     GLPAFPVPSERVIDTNGAGDVFHGAYIYSYLANPGKSWHDHFDFARAASTHKIQRLGN
                     EAGLPTLADIEKVRQEFEVRV"
     gene            1961..2830
                     /locus_tag="VM910_13505"
     CDS             1961..2830
                     /locus_tag="VM910_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI63582.1"
                     /translation="MITGLDHVVVLLEDIDQGRIAYETLLGCAPAWQNSGDGADRVMF
                     TLDNMTLELMAPHGLSAAADRIREVIKTSGIGLASMCFRASDIFKMHRRLDRVALKPD
                     PVAEVESRDTASGATLRWKRTRASTDLTRGVRMFFLELTGERPRSAAVAGAPVLGLDH
                     VVISTQHPEGAAALYGARLGLDMALDRTHQDWGQLMFFRCGDLIVEVVRRRVASGDTA
                     PKNQDVNQDDKLWGLSWRVADIEAARARLSASGIDVSDVRMGRKPGTRVMTVRSGTCG
                     VQTLFLERTPRVD"
     gene            2900..3607
                     /locus_tag="VM910_13510"
     CDS             2900..3607
                     /locus_tag="VM910_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI63583.1"
                     /translation="MAKAKRVLRWQRDPEGMRLRILEAAKQEFATHGLAGARVDRIAA
                     KAGANKRMLYYHVGNKEDLYLTVLEGAYEKIRAEERSLDLEHLDPPEAIRRLIDFTWN
                     YFLRNPEFLALLNTENLARAQHLKRSTKVKLMHSPFVEMIRTVVRRGVEAGDFPVEVD
                     PVQLYISIAALSFFYLSNSATLSVFFGRDLLAREAKDERLDHMVALVLAALTGKSSAD
                     FGRNKMPRFRVAADQTA"
     gene            3697..>4158
                     /locus_tag="VM910_13515"
     CDS             3697..>4158
                     /locus_tag="VM910_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63584.1"
                     /translation="MPLRFTLHDVALFERPVRFARPFRFGAVTINAAVQAFVRVEIEV
                     EGKGRATGASAELLAPKWLDKRPHLSSEETLAEMRRSLTIARDLYLAHSGFATAFGIH
                     AAVIGAQVEVCAGEDIPRLAAAYGPAEIDKAILDALLRAAGANVFDGMRQNI"
BASE COUNT          757 a         1282 c         1331 g          788 t
ORIGIN      
        1 tcgttgcacg caaatggagg ccgcaacatt ggcataccca agcgcatctt gaaccggctg
       61 gttgtcggcg agccgagccg ccagcgcgcc aacgaagcaa tcgccggcgc cggtgctgtc
      121 gacggcctcg acttttcgac ccgggacgat cagcggcttg ccgttgacga gcgcgaccag
      181 cccgcgctcg ccgagcgtca cgcagatgat ctgatcatca tgcctccgca gcgatctggc
      241 cgcgtcggca aaccgcgcat cgtcatcgct accgcttagc gccacgcgcg ccagcaaggc
      301 cagctcggtc tcgttcagca cgaggatgtc gacgagatcc agcaattcgg catcaagcgc
      361 gcgagctggc gctgggttga gcatggtgga tgcgcccgcg gcgcgcgcac ggcggaagaa
      421 ggcattgatg gtggccaggg gaatctcgaa ctggctgacg gcgatgtcgc ccttcccaag
      481 cacggccgat gcgacatcgt ccggcgtgac caggtcattg gcgcccggca ccaccacgat
      541 cgtgttctca gcattggcaa gcgtgatgat agcggtgccg gtgtgggcat cagcggtgtc
      601 ccgaaccagg cagaggtcga taccctggtc ggcgagaaac gtccgcagct cccgaccgaa
      661 tgcatccttg ccgagccgtc cgatcagcgt tgtcgcggcg ccaagcttgc tggcagcgac
      721 cgcctggttc gcgcccttgc cgccgggaaa gtaaagaacc tccttgccgg caacggtctc
      781 gccgagtgcc gggtgccgct cggcgatcgc caccacatcc atgttgatgc tgccggcgac
      841 gaacacgcga cccatgaaag aacccgatca gacccggact tcgaactcct gcctgacttt
      901 ctcgatgtcg gcaagggtcg gcagtcccgc ctcattgcca agccgctgga tcttgtgggt
      961 cgacgcggcg cgcgcgaaat cgaaatggtc gtgccagctt ttgccgggat tggcgagata
     1021 ggaatagata taggcaccgt gaaagacgtc gcccgcgccg ttggtgtcga tcacccgctc
     1081 gctggggacc ggaaacgccg gcaatccatg caccgctccg gtctcgtcgt accagagcaa
     1141 tcccttctcg cccaatgtga ctccgccgac ccggcagccg cgctccttga gataatcgag
     1201 catcttttcg atcgtcagat ccatctgctc gcacagccgc tcggcgacga tggcgacgtc
     1261 gatgtactgc aggaggtcat gcgtattggt gcgcaggccg ccgccgtcga gcgaagtcag
     1321 gattccctcc tcgcggcaga gctttgcgta gtgaattgcc gcatccggct ggtggccgtc
     1381 gatatgcagc gcgcggcaat tgccgaggtt gaggagcggg aagggatgga tatgcgcatc
     1441 gtcgcggcag cgcacgatgg cgcgcttgcc gtccttcggc atgatgaagg agagcgagga
     1501 ggcgttgacc ttgcgcggat gcacggaaat tccgtacttc gcgctcatgt ccatgaacat
     1561 gcgcccgagc cagtcgttgg caacggtggc gatcaggtcc ggaacgatgc cgagcttggc
     1621 acagcagaac gccgcggtga ccgcgttgcc gccgaaggag accgcatagg cttcggcgac
     1681 gtgcttttca tcgccggtcg gcagatggtc ggtaatgaag gtgacatcga tataggtttg
     1741 tccgataaag agggcctgca taaggttttc cgtcatgcgc gtggagaggt ggtcccggcc
     1801 ggctgcaaca gattagcacc ggtttttccg ggcattagct gaaattatgc gcaaagactg
     1861 cccccataat cgcttgtcgt gaggccttgt gccgggcatc gtcatcccat cccgccgcca
     1921 taacagcctg cggccggctt cggttccgga ggaaagaagc gtgatcactg gtctcgatca
     1981 tgtcgttgtt ctgctcgagg acatcgacca aggcaggatc gcctatgaga cgctcttggg
     2041 ctgcgcgccg gcctggcaaa acagcggcga cggtgccgat cgcgtgatgt tcacgctcga
     2101 caatatgacg ctggaattga tggctcctca cggcctcagc gcggccgccg accgcattcg
     2161 cgaggtcatc aagacatccg gcatagggct tgcgagcatg tgtttccgcg cctcagatat
     2221 cttcaagatg catcgccggc tcgatcgcgt cgcgctgaag ccggatccgg ttgcggaggt
     2281 cgagagccgc gataccgcct cgggcgcgac cttgcgctgg aagcgcacgc gcgcatcgac
     2341 cgatctgacg cgaggtgtac gtatgttctt tctcgagttg accggcgagc ggccgcgctc
     2401 ggccgcggtc gcaggcgcgc cggtcctcgg gctcgaccat gtcgtgattt ccacacagca
     2461 cccggagggc gccgcggcgc tctatggcgc gcgccttggc ctcgacatgg cactcgaccg
     2521 gacccatcag gactggggtc aactcatgtt ctttcgctgc ggcgatctca tcgtcgaggt
     2581 cgtgcgcaga cgggtcgcga gcggcgatac ggccccgaaa aaccaggacg taaaccagga
     2641 cgacaaattg tgggggctga gctggcgcgt cgccgacatc gaagcggcgc gcgcacggct
     2701 ctccgcctcc ggaatcgacg tttccgacgt gcgcatgggc cgcaagccgg gcacccgggt
     2761 gatgactgtg cgcagcggca cttgcggcgt ccagacgctg ttcctggaac ggacgccgcg
     2821 cgtggactag ccgggcgcgg ttctcaaacg gccggttgca tggtacatcc cgttgcgtag
     2881 cacagtccga gcgatcgttt tggccaaggc gaaacgagtt ttgagatggc agcgcgaccc
     2941 cgagggtatg cggctgcgca ttctcgaggc ggcgaaacag gaatttgcca cacacggcct
     3001 tgccggtgcc cgcgtcgacc ggatcgcggc gaaagccggc gccaacaagc gcatgctgta
     3061 ctaccacgtc ggcaacaagg aagaccttta tctcacggtg ctggagggcg cctacgagaa
     3121 aatccgcgcc gaggagcgca gcctcgatct cgaacatctc gatccgcccg aggcgatcag
     3181 gcggctgatc gatttcacct ggaactattt tttgcgcaat ccggaatttc tggcgttgct
     3241 caacaccgag aacctggcgc gggcgcagca tctgaagcga tcgaccaagg tcaaattgat
     3301 gcattcgcct tttgtcgaga tgatccgcac ggtagtgcgg cgcggcgtcg aggccggcga
     3361 ctttccggtc gaggtcgatc cggtccagct ctatatctcc atcgctgcct tgagcttctt
     3421 ctatctgtcg aacagcgcaa cgctcagcgt tttttttggc cgcgacctgt tggcgcgtga
     3481 ggccaaggac gagcggctcg accatatggt cgcgctggtg cttgcggcat tgaccgggaa
     3541 atcgtccgcc gactttggca ggaacaagat gccgcggttc cgcgtggcgg ccgatcagac
     3601 agcttgatcg atatttcctt cgccggggag ttttgcgccg agggcttttg gacctagaca
     3661 gtatttatcc aacaggttaa tttcggatcc acgacgatgc cgcttcgctt caccctgcac
     3721 gacgttgctc tgttcgaacg gccggtccgc tttgcccggc ccttccgctt cggcgcggtc
     3781 acgatcaacg cggccgtgca ggctttcgtg cgggtcgaaa tcgaggtcga aggcaagggc
     3841 agggcgacag gtgccagcgc cgagcttttg gccccgaaat ggttagacaa gcggccgcat
     3901 ctttcatccg aagaaacctt ggccgagatg cggcgctcgc tgacaatcgc gcgcgatctt
     3961 tatctggcgc attccggctt tgcgaccgca ttcggcattc atgcggccgt gatcggcgcg
     4021 caggtcgagg tttgcgccgg ggaagatatc ccgcggcttg cggccgctta cgggccggcc
     4081 gaaatcgaca aggcgatcct cgacgcgctg ctgcgcgctg ccggcgccaa tgttttcgac
     4141 ggcatgagac agaatatc
//
LOCUS       DATLJT010000582         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4400033,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000582 DATLJT010000000
VERSION     DATLJT010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4400033"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VM910_13520"
     CDS             <1..633
                     /locus_tag="VM910_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI63585.1"
                     /translation="YPRMGEYIQVIKGLWTDSDFNFDGTYYRAHVRAALTGADGKVVI
                     PDQGDIVAKASHLPHPPIYAASRSPQGKELIAQHGDVWFAGYKPGYRNFESNIECMAV
                     DVRAMDELAARHGRKLRYAINPQVICCETQPEAERLADEAERNAGPRDRMVNALGAGL
                     VGTPQLLAERLRRYGEVGIEIMMTRFTPMMEGMETFGTKVMPLLSREATA"
     gene            complement(1708..>2533)
                     /locus_tag="VM910_13525"
     CDS             complement(1708..>2533)
                     /locus_tag="VM910_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63586.1"
                     /translation="QEKLGQPVVIENRPGAGGTLGNEVVARADKDGYTLGIMTAGQII
                     AAVMSKSPRYDTLTAFDPVSQVATAGLIIVTRPDFPADDVKQLIAAAKANPGKISFAS
                     PGFGATQHFTGELFRQTAGVNILHVPFRTSPEAISAVLGKQVDVLFDTVSAVLGQVQS
                     GQLKALAVTGKDRFPAVPDVPAAIESGVLPGYDITTWYGVFAPRGVPGEIIAKLNTTI
                     NESLKEDAVQRRLTTAGVVVRGSSAEAFGEHMAREFKRWNAVREAAGIPQQWQRSL"
BASE COUNT          525 a          751 c          747 g          510 t
ORIGIN      
        1 tatccgcgca tgggcgagta catccaggtg atcaagggcc tgtggaccga ttccgatttc
       61 aattttgacg gcacatacta tcgcgcgcat gtgcgggcgg cgctgaccgg tgccgacggc
      121 aaggtcgtca ttcccgatca gggcgacatc gtcgccaagg cgagccatct gccacacccg
      181 ccgatttatg ccgccagtcg ctcgccccaa ggcaaggagt tgattgcgca gcacggcgac
      241 gtctggttcg ccgggtacaa gcctggttat cgcaatttcg agtcgaatat cgaatgcatg
      301 gcggtcgatg tgcgcgcgat ggacgagctt gccgccagac acggacggaa gttgcgctac
      361 gcgatcaatc cgcaggtcat ctgctgcgag acgcagccag aggccgaacg acttgccgac
      421 gaggccgagc gcaatgccgg accgcgcgac cggatggtca atgcgctggg agcggggctt
      481 gttggaacac cgcagctgct cgccgagcgc ttgcgccggt acggggaggt cggcatcgaa
      541 atcatgatga ctcgcttcac tccgatgatg gagggaatgg aaactttcgg caccaaggtc
      601 atgcccctac tcagtagaga agcgaccgcg tgacggtatg aacacgaatc tcaaatactc
      661 cgccgtccta tccgtactgg gcatcggttt attttctaca ccctctgtcc acgcccagga
      721 caactatccg tggaagccga tccagctcgt ggtcacgacc gcggcaggcg gcgctcggat
      781 gaaggcagtg ccgtggccca gtgcaatgcc tacatcccat tcataggtgt gaggaccatc
      841 ctcttatatc tatgtcatgc ttcacccgga tcggaaacta ctaatcggca caatgtgtgc
      901 tctgggcgct ctgttctggc cggtcacaat gattatagga tacatatcgt gaagaaactt
      961 ctgctcgcca gttgcttggt gttggcgggg ttggtgcctg caaaggctga ctcgcttaca
     1021 gacgctctta cagacgcttt gaagtggcaa caaggagcat taccgaagga tgtaattgga
     1081 acgtggtgtc atacaggagc aatgacaggc caatttaagc gcagcaaaga ttgctcacct
     1141 ctaaaaaagt cacaatcact cgggaagggc tcaaggagtg ggaggggtat tgcaagttct
     1201 ttagtgtcca cgttcacaaa aattctgaag ataaaaacga cagaacgttt cgaggacatg
     1261 cccgagacct caccacaata atccaaatca taagtttgtg tgaggaggga gaagataaga
     1321 aagcgtctgt agttgaactc cgccaacctg tacaggataa atccctcctt agtattgagc
     1381 ggagagatga agcaggagac ccaagctgtc gtgaccgctc ttgtgattag ctatcaagtc
     1441 ccggatcatg atcagtagtc cccatctcgt caccactatt gcggtgtgct ccataaccac
     1501 tatcgtttcc acctgggcgt tcatcctcgg gatcatgacg cggatttgat ttctcgccct
     1561 tctctacttc cttcggagcg ggcttatcat catccttcca gcgaggcgca ataggatcac
     1621 ctaatcttcg cgttgagtcc gcaaagcggg ccaaaacgga cattaatctg gtcgctgtca
     1681 ccaatcgcga tttttttgag gacacgccta tagagaacgc tgccactgct gcggaatgcc
     1741 cgcggcctcg cgcaccgcgt tccagcgctt aaactcgcgc gccatgtgct cgccgaacgc
     1801 ctcggcgctc gagccgcgca cgaccacgcc ggccgtggtc aggcgccttt gcacggcgtc
     1861 ctccttgagg ctctcattga tggtcgtgtt gagcttggcg atgatctcgc ccggcacgcc
     1921 gcgcggcgcg aatacgccgt accacgtggt gatgtcgtag ccgggaagca cgcccgactc
     1981 gatcgcggcc ggcacgtccg gcaccgccgg aaagcggtcc ttgccggtga ccgcgagcgc
     2041 cttgagctgg cctgactgca cctgccccag caccgccgag accgtatcga acagcacgtc
     2101 cacctgcttg ccgagcaccg ccgagatcgc ctcgggcgag gtgcgaaacg gcacgtgcaa
     2161 gatgttgacg cccgcggtct gccggaacaa ttcgccggtg aagtgctgcg tggcgccgaa
     2221 gcccgggctg gcgaacgaaa tcttgccggg attggccttg gcggcggcga tcaattgctt
     2281 gacgtcgtcg gcaggaaagt ccgggcgcgt gacgatgatc agcccggcgg tcgcgacctg
     2341 cgacaccgga tcgaacgcgg tgagcgtgtc gtagcgcggc gacttgctca tcaccgccgc
     2401 gatgatctgg cccgcggtca tgatgccgag cgtgtagccg tccttgtcgg cgcgggcgac
     2461 aacctcgttg cccagcgtgc cgccggcgcc cggccggttc tcgatcacca ccggctggcc
     2521 gagcttctcc tgc
//
LOCUS       DATLJT010000583         6184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1184055,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000583 DATLJT010000000
VERSION     DATLJT010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184055"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VM910_13530"
     CDS             <1..194
                     /locus_tag="VM910_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI63587.1"
                     /translation="MADLADGILEGDVVECSLHFGAFHVPSGKAVREPCFTDLKTFRT
                     EVKDGQVYVDLSAPAATAS"
     gene            complement(155..2830)
                     /locus_tag="VM910_13535"
     CDS             complement(155..2830)
                     /locus_tag="VM910_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVI63588.1"
                     /translation="MLQQAIVTIVDFCTRCAAQIIGVGVLLGLATAVYAAHHFAIDAD
                     VNKLISKELPWRQREVAFEKSFPPKEETILAVIDAPTSELATQATAALIHKLSDQKDH
                     FRSILEAGGGPFFQKNGLLFLPTGEVVALTKKLGEAKPLIQTLAQDSNLRGLTTALNY
                     GLIGARMNQYTLDNLSGTFNMVSDTLEEAIAGRPASFSWRAMLNGRPPTPDERRRFLE
                     IRPVLDYAALMPGKVATDAIRQAAADLDIGAKYGATVRLTGPVAIADEEYATLEEGAY
                     TNTAVTIVVVLTILWLALRSFGIILAVAISLVVGLSITAAVGLALVGALNLISIAFAV
                     LFIGLGVDFGIQFSVRYRAERHEVDDLHQALFNTASHVGAPLTLAAAATAAGFLSFLP
                     TDYKGLSELGLLAGLGMIIAFLTSITLLPALLTVLRPPGEPKEMGFKSLAPLDRFMER
                     YRIPIIVGTGLVVAAGLPLLYWLQFDFNPLNLRSTRVESVATLLELRSDPAIGASSIF
                     VLAPNKEIAQSDLEKLSKLPEVSSVKTIESFVPSDQQPKLAAIRQLATVLDPVLRPDP
                     NKKPPTDADNVAALKAAVDALKQSAGSQTGPGATAAKRLADDLAKLASGDEAMRGRAH
                     AAMIPSLNTALDELRNYLQAQPVTLETLPPEIAREWVTQDGRYKVEILPKGDPTDNET
                     LREFARAVQAVEPNATGGPIAILESGRTVVRAFFEAGFWALGSIVILLWIVLRRFGDV
                     LLTIIPLLMAGVVTLELMVLFGTKLNFANIIALPLLLGVGVAFKIYYIMAWRAGQTDL
                     LQSSLTRAVMFSALTTATAFGSLWLSSHPGTSSMGKLMALALVTTMAAAVLFQPVLMG
                     PPRDPKPKRFKRLIRAGAAAARSQEAVAAGADKST"
     gene            3220..4146
                     /gene="ispH"
                     /locus_tag="VM910_13540"
                     /gene_synonym="lytB"
     CDS             3220..4146
                     /gene="ispH"
                     /locus_tag="VM910_13540"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVI63589.1"
                     /translation="MKVILAQPRGFCAGVVRAIEIVERALKKYGPPVYVRHEIVHNKF
                     VVDSLKAKGAIFVEELSEVPQGAVTIFSAHGVAKSVVNEAAGRQLPVLDATCPLVTKV
                     HNQGKRYVAQGRVLILVGHAGHPEVEGTLGQIDGPVVLVQSEEDVAALDISPETPVAY
                     VTQTTLSVDDTRGIIAALERRYRNIVGPEIRDICYATQNRQSAVRELSHHVDVILVVG
                     AKNSSNSNRLREIGAEAGIPSYLIADGSELAPEWVKGAQTIGLTAGASAPEVLVEDVI
                     NTLRRLGEVEVSTLPGREEHIEFRLPRELAAG"
     gene            4208..5347
                     /gene="hpnH"
                     /locus_tag="VM910_13545"
     CDS             4208..5347
                     /gene="hpnH"
                     /locus_tag="VM910_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HVI63590.1"
                     /translation="MRVPFFKEMRIGAYIFKQKLLGRKRYPLVLMLEPLFRCNLACVG
                     CGKIDYPDPILNRRLSVKECLDAVDECGAPMVAIPGGEPLIHREIGEIVKGLVAKKKF
                     VSLCTNALLLEKKLHLFEPSPYLFFSVHLDGLREHHDKSVCQPGVFDRAVSAIKAAKA
                     KGFVVNVNATIFDGHPAEDVAAFLDYAKELGVGVSISPGYAYERAPDQEHFLSRRKTK
                     ELFRKVFNMGQGKNWNFIHSNLFLDFLAGNQTYHCTPWGMPTRNIFGWQKPCYLLGEG
                     YAKTFRELMQTTDWDSYGTGKYEKCANCMAHCGYEPTAAAATMSHPWQALKVAMFGPR
                     TEGPMTPEIPLDKQRPAEFVFSRHVEHELAAIQQAEKRAPLEAAE"
     gene            complement(6018..>6184)
                     /locus_tag="VM910_13550"
     CDS             complement(6018..>6184)
                     /locus_tag="VM910_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63591.1"
                     /translation="GDDGFWSEPRYTATGFPRVFYLRYHGYSKLFPLWALARYRNLKN
                     ANMCSVRYGM"
BASE COUNT         1205 a         1906 c         1981 g         1092 t
ORIGIN      
        1 ccatggccga cctcgccgat ggcattctgg agggcgacgt cgtcgagtgc agcctgcatt
       61 tcggcgcgtt ccacgtgccg agcgggaaag ccgtgcgaga gccctgcttc accgatctca
      121 agacgttccg gaccgaggtg aaggacgggc aggtctatgt ggacttgtcg gcgccggcag
      181 cgaccgcttc ctgagatcga gccgcagcgg cgcccgcgcg gatgagacgt ttgaaccttt
      241 ttggcttggg gtcgcgcggg gggcccatga gaaccggctg gaacagcacc gccgccgcca
      301 tggtggtgac gagcgccagc gccatcaact tgcccatgct cgatgtgccg gggtggctcg
      361 agagccatag gctaccgaac gcggtggcgg tggtgagcgc gctgaacatc acggcgcgcg
      421 tcaggctcga ctgcagaagg tcggtttggc cggcccgcca cgccatgatg taatagatct
      481 tgaaggccac gccgacgccg agcagcaacg gcagcgcgat gatgttggcg aaattgagct
      541 tcgtgccgaa gagcaccatc aactccaggg tcacgacacc cgccatcaac aagggaatga
      601 tggtgaggag gacatcgccg aagcggcgca gcacgatcca gagcagaatg acgatcgagc
      661 caagcgccca aaaaccggct tcgaagaacg cccgcacgac ggtgcgcccg gactcgagaa
      721 tggcaatcgg gccgccggtg gcattcggtt cgactgcctg caccgcgcgc gcgaattcgc
      781 gcaacgtctc gttgtcggtc gggtcgcctt tcggcaggat ttccaccttg taacgcccgt
      841 cttgcgtgac ccattcgcgc gcaatctcgg gcggcaaggt ttcgagcgtg accggctgcg
      901 cctgcaggta gtttcgcaac tcgtcaagcg cggtattgag cgaggggatc atggcggcgt
      961 gtgctcgccc gcgcatcgcc tcgtctccgc tcgcaagttt cgcgaggtcg tccgccaacc
     1021 gtttggctgc ggtcgcgcca ggtccggttt ggctgccagc tgattgcttc aaagcatcga
     1081 ccgcggcttt gagcgcggcg acattatcgg cgtccgtcgg cggttttttg ttggggtcgg
     1141 gccgcagcac tggatcgagc acggtcgcga gctggcggat ggcggcaagc ttgggctgct
     1201 ggtcactggg gacgaaactt tcgatggtct ttacgctcga gacctccggc agcttggaaa
     1261 gcttctcgag atctgactgc gcgatctcct tgttcggcgc cagcacaaaa atcgaactag
     1321 cgccgatggc ggggtcgctt cgcagctcca ggagcgtcgc aaccgactcg accctggtgc
     1381 tcctcagatt gagcggattg aaatcgaact gcagccaata caagagtggc aggccggccg
     1441 cgacgacgag cccggtaccg acgatgatgg gaattcggta ccgctccatg aagcgatcga
     1501 gcggcgccaa gcttttaaag cccatttctt tcggttcgcc cggcggccgc aacaccgtca
     1561 acaatgccgg aagcagggtt atgctcgtca ggaacgcgat gatcataccg aggccagcga
     1621 gcaagccgag ttcggacaat cccttgtagt cggtcggaag gaacgacaga aagccggcgg
     1681 cggttgcagc cgcggcaagc gtcagcggcg cgcccacgtg gctcgcagta ttgaatagcg
     1741 cctgatggag atcgtcgacc tcgtgacgct cggcgcgata gcgcacgctg aactgaatgc
     1801 cgaagtcgac gccgagcccg ataaacagaa cggcgaaggc gatcgaaatc aggttgagcg
     1861 cgcccaccag cgccaagccg accgcggcgg taatggagag gccaaccacc aagctgatgg
     1921 cgaccgccag gatgatccca aacgaacgca aggcaagcca gaggatggtc aacaccacca
     1981 cgatggtcac ggccgtgttg gtgtacgccc cctcctccag cgtggcgtat tcctcgtcgg
     2041 cgattgcgac cggtccggtc aggcgtactg tcgcgccgta tttcgcaccg atatccaaat
     2101 cggccgccgc ctgccgaatg gcatcggtcg ccaccttccc cggcatgagc gcggcgtagt
     2161 cgagcaccgg cctgatctcg aggaagcgcc gccgctcgtc cggggtcggc ggcctgccgt
     2221 tgagcatcgc gcgccaggag aagctcgccg ggcgccccgc gatggcctcc tcgagcgtgt
     2281 ccgagaccat gttgaaggtc ccggaaagat tgtcgagcgt gtactgattc atccgcgcgc
     2341 cgatcaatcc ataattgagc gcggtggtca ggccgcgcag gttggaatcc tgcgccagcg
     2401 tctggatcag cggcttggct tccccgagct tcttggtgag cgcgacgacc tccccggtgg
     2461 gcaagaacaa aagtccgttc ttctggaaga atggcccgcc gccggcctcc aggatcgagc
     2521 ggaagtgatc cttctgatcg gacaatttat ggatcagcgc cgccgtggcc tgcgttgcca
     2581 attcggaggt cggagcgtca atgacggcaa gaatggtttc ttccttcggg gggaacgact
     2641 tttcgaatgc cacctcgcgc tggcgccacg gcagttcctt ggaaatgagc ttgttgacgt
     2701 cggcgtcgat ggcgaaatgg tgggccgcat agacggccgt ggcgagaccc aaaagtaccc
     2761 cgacgccaat gatttgcgcg gcacagcggg tgcagaaatc aacgatagtg acgatcgctt
     2821 gctgcagcat gcctagtcct ggggggctat tgcgactggg atccaatcgg aaggaaacgt
     2881 ggacgagtat ggcggcggaa tatcaggcaa tgcagggcgt tggcaacgac tacaggttgc
     2941 acgggaacac tgccagaaca ccggttaacc ccaaagggcg cgccaagttc gacgagcgcg
     3001 acgatgggtc gccgcaaccg cagattcccg ctgatttggg ctgtgcctgc ggcaacctat
     3061 atgggaaaat gttggaatca tgaccccgcc gctgtccctg ccggatcctg tcgcacgctc
     3121 cccccggtcg gtcgatcacg accgtccggc gccgatgtat gccaaagcct gcaccgcggc
     3181 gcggcgcgtg cggagcagga gcgccccagc gagacgctca tgaaggtcat tctggcgcaa
     3241 cctcgcggat tctgcgcagg cgtcgtgcgc gctatcgaga tcgtcgagcg cgcgctcaag
     3301 aaatacgggc cgccggtcta cgtccgccac gagatcgttc acaacaaatt cgtcgtcgac
     3361 agcctcaagg ccaagggcgc gattttcgtc gaggaactat cggaagtgcc gcagggcgcc
     3421 gtgaccatct tcagcgcgca cggcgttgcc aagagcgtgg tgaacgaggc ggccggacgt
     3481 cagctgcccg tgctcgatgc gacctgcccg ctggttacga aggtgcacaa ccagggtaag
     3541 cgctacgtcg cccagggtcg cgtgctcatt ctcgtcggcc acgccggcca tccggaggtc
     3601 gagggtacgc tcggccagat cgatggtccg gtggtcctgg tgcagagtga ggaggacgtc
     3661 gcggcgctgg acatttcccc tgagacgccc gtcgcctatg tgacgcagac gacgctaagc
     3721 gtcgatgaca cccgcggcat cattgccgcg cttgaacgcc gctaccgaaa catcgttgga
     3781 ccggagatca gagacatctg ctacgccacg caaaatcgcc agtcggcagt gcgggaactg
     3841 agccatcacg tcgatgtgat cctggtggtc ggcgccaaga atagttccaa ttccaatcgg
     3901 ctgcgcgaaa tcggcgcgga ggccggcatt ccgagctatc tcatcgccga cggcagcgag
     3961 cttgcacctg agtgggtcaa gggtgcgcaa acgatcggcc tcacggccgg cgcctccgcg
     4021 cccgaggtgc tggtcgaaga cgtcatcaac acattgcgaa gactcggcga ggtcgaagtc
     4081 tcgactctcc cgggccgtga ggagcatatt gagttccgcc tcccgcgcga actggcggcg
     4141 ggctgagacg ttcaccagac agccgcagcc tgagacggca ataaaaacag acaggaagac
     4201 agaaagaatg cgagttccgt ttttcaaaga aatgcgtatc ggcgcctaca tcttcaagca
     4261 gaaactcttg gggcgcaaac gctatccgct cgtgctgatg ctggagccgc tgtttcgctg
     4321 caatcttgcg tgcgtcggtt gcggcaagat cgactatccc gatccaatcc tcaatcgccg
     4381 gctgtcggtc aaggaatgcc tcgacgccgt cgatgagtgc ggtgcgccga tggtggccat
     4441 ccccggcggc gagccgttga tccaccgcga gatcggcgag atcgtgaaag gcttggtcgc
     4501 caagaagaag ttcgtctcgc tgtgcacgaa tgcgctgctc ttggagaaga agctgcatct
     4561 gttcgagcct tcgccctatc tcttcttctc cgtccacctc gatgggctgc gcgagcatca
     4621 cgacaagtcc gtctgtcagc cgggcgtgtt cgaccgcgcc gtgtccgcga tcaaggccgc
     4681 caaggccaag ggcttcgtcg tcaacgtcaa cgccacgatc tttgacggcc atccggccga
     4741 agacgtcgcc gccttcctcg attacgccaa agagctcggc gtcggcgtgt cgatctcgcc
     4801 cggctatgct tatgagcgcg cgcccgacca ggagcatttc ctcagccggc gcaagaccaa
     4861 ggagcttttc cgcaaggtct tcaacatggg ccagggcaaa aactggaatt tcatccactc
     4921 gaacctgttc ctcgatttcc tcgcgggcaa ccagacctat cactgcacgc cctggggcat
     4981 gcccacccgc aacattttcg ggtggcagaa gccctgctac ctgctgggcg aaggctacgc
     5041 caagacgttc cgcgaattga tgcagaccac ggactgggac tcctacggca ccggcaagta
     5101 cgagaagtgc gcgaactgca tggcccattg cggttacgag ccgaccgcgg ccgcggcgac
     5161 gatgtcgcac ccctggcagg cgctcaaggt cgccatgttc ggcccacgca ccgaagggcc
     5221 gatgacgccg gaaattccgc tcgacaagca gcgcccggcc gaattcgtgt tctcgcgcca
     5281 cgtggagcat gaactcgcgg ccattcaaca ggccgagaaa cgggccccgc tcgaggcggc
     5341 ggagtaaaac aaccctttcg ttggtcatcc ggccgagccg cgacagcggc gaggccaatc
     5401 cgcaccacgc cagagcgcgc cgacgcgctg gcgggcaagg cgcgttgcag atttcgcttg
     5461 acgtgcgtaa ccagaatctt tcagaaaagc atattcacca aatttgcgct cggaagccgg
     5521 cccgctgccg gttcgatgaa cgttggtcgt tggcgcccgg ccatggtgac ggcgctgcgg
     5581 ggacctcctt gcacgcggat cgtccgtctc cctgcacttt gcctgttatc gcattgttat
     5641 ttttctcggg cccgttggcg acagcaaccg aagtcggcag tccccgcgca cgactcgagg
     5701 ttgcagtagg aggctaaggc attggagaca cggcggaaag tcgattgggc cgcggcgccc
     5761 tgccgcgctg ccgacaagca aggcgcaacg ggtggggatg gctcccgttg ttttccgatt
     5821 gttatttaca atgcgggttg tagcggatcg cacgcgcaac tacagcccgg ggttgaacgc
     5881 gaaagatgac cggcagtcgc gcgcaagagc gaagcgtatg tgagttcgtc attgcggccg
     5941 agcccgcgat ggcggcgaga cccatactca ctggccgtgg gggcttgcgg gcgtagccgg
     6001 cgccgcggaa tgacgcgtca catcccgtac cgcaccgagc acatattggc attcttgagg
     6061 ttgcggtagc gcgcgagcgc ccacagcggg aagagtttcg agtagccgtg ataacgcagg
     6121 tagaacacgc gcgggaagcc cgtcgccgtg tagcgcggct cgctccagaa gccgtcgtcg
     6181 ccct
//
LOCUS       DATLJT010000584         1855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3481674,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000584 DATLJT010000000
VERSION     DATLJT010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481674"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VM910_13555"
     CDS             complement(<1..248)
                     /locus_tag="VM910_13555"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HVI63592.1"
                     /translation="MTLYGGVVPYPFAKTKAITHQLVDACADRPEGWSSVFPEKVKDV
                     VLPGYTVFNLDDARVAARRLLDLGPIRIKEPLAAGGKG"
     gene            complement(1122..1436)
                     /locus_tag="VM910_13560"
     CDS             complement(1122..1436)
                     /locus_tag="VM910_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63593.1"
                     /translation="MALLDARPALMQRSSPRGRLRPIDLGLLWIAELDAACLGSGQRR
                     ACALADHVSFVLSSGGEHVKRQPVGPGACRRRAASRCDRPQLQAAMALAKKRKASLLV
                     IA"
     gene            complement(1668..>1855)
                     /locus_tag="VM910_13565"
     CDS             complement(1668..>1855)
                     /locus_tag="VM910_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63594.1"
                     /translation="ISLLYEKQRVLHRRGLDQLEGEMMAFSREWDRAVDGSPNRLDAM
                     IWGITRLSKIITYIPIA"
BASE COUNT          378 a          572 c          559 g          346 t
ORIGIN      
        1 tgacctttgc ccccggctgc gagcggctct ttgatccgga tcggaccgag gtccaggaga
       61 cgtcttgccg caacccgggc gtcgtccaag ttgaaaacgg tgtaacccgg caacacgacg
      121 tccttgacct tttccgggaa gacagacgac cagccttcag gtcgatccgc gcaggcgtcg
      181 actaactgat gagtgatcgc ttttgtcttc gcgaaaggat acgggacgac gccgccgtag
      241 agggtcattc gggcctcgga tcccgaggca cgcagcctca tctgcaatca atgtgtcgtc
      301 cggaacaaag aacacacgcc cctcggcgtc gtcgaagcga gctgaaaact cgcaatgttt
      361 gatctcagcg atcgcttcgg caaccgcgga aagtaccacc ctttcgtggg tgtacatgaa
      421 cccgcccagc cgggagaagt agacgacgac cgcgcccgcc gacatgacta tcgctccgcg
      481 cgcgaagtct ggcgccgggt tggaacatgg cttttcgccg agccctccat gcctcggctg
      541 cgactcggac cgaacggcct ggggttgatc tgatagggcg caggtaatcg cccggaagcg
      601 gggatggttt gaacttccga gactgagcga ggaactgcgg cggtgaccgt gatgcgcgca
      661 gccgtgaaac tgcaactctt gatcaacatc gaacaactcc cactaggcga cctcttacat
      721 ccgagagtcg cctttatgat acaagtgttt cactcatcac ttatgcgtga cacgtatgtt
      781 tcgctttgcc gcggccaccg cggcgcgtac cccagagcga agtgatctgc gtttaaggca
      841 gaattaaatg tcgatcacag aacagtgagt cagcgcgtaa gctgcagcgc ttaagtgcga
      901 cgctgcacag gtggtagcga tctcacggaa aatagctgtg gcgctcgcgc ggcattcgca
      961 aaaaagctgg cggcggggac ctgaacgatg tccgctttgg gggtcaggat cggtcctctg
     1021 acgatgtccg ccgcacgacc gctttggccc tcgttcacgt cgtgatgtct cgaggtgcca
     1081 ggacccgact catgcaacac ggcgcatcag gacgcttcgg ctcaagcgat caccagcaag
     1141 ctggccttgc gcttcttggc caacgccatc gcagcttgca actgcggccg gtcgcagcgc
     1201 gaggccgcgc gccgccgaca tgccccgggg ccaaccggct gacgcttcac gtgctcgccg
     1261 ccgctgctga gcacgaacga cacatgatcg gcgagcgcac acgccctcct ctggccgctg
     1321 ccaaggcacg cggcgtcaag ctcggcaatc cagagcaggc caagatcaat cgggcgaagg
     1381 cggccgcgcg ggctcgagcg ctgcatcaag gctggccgcg catcaagcag tgccatcgca
     1441 accgatctca acgcgcgcgg catcgcgaca cggaatggcg gcgaatggtt tccggtgcag
     1501 gcgcatcgcg atcgtctcgg cctatgatcg accggggggt tgctttgccg agcgtgaaag
     1561 ttttttcgcc cgcccgggag caccccctcc gcacacggtt tgcgaatatt tcactgcgca
     1621 gggcggagtt ccgaaaagcg aatttcgatg gcgccgcaag gggacaatta tgcgatcgga
     1681 atgtacgtga tgattttact cagccgagta attccccaga tcatggcatc aagccggttt
     1741 ggcgagccat caacggcgcg gtcccactcg cggctgaagg ccatcatttc gccctcgagt
     1801 tgatcgagcc cgcggcgatg caggacacgc tgcttttcat ataatagact gatcg
//
LOCUS       DATLJT010000585         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2102670,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000585 DATLJT010000000
VERSION     DATLJT010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102670"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VM910_13570"
     CDS             complement(<1..227)
                     /locus_tag="VM910_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63595.1"
                     /translation="MDWNFLFVNILNGVVYGAFLLLTSLGLSLVFGLGRVVNFAHGAL
                     YAMGGYALIAVMYGVGVGYWLGLVVAPLLVIP"
     gene            complement(267..1424)
                     /locus_tag="VM910_13575"
     CDS             complement(267..1424)
                     /locus_tag="VM910_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63596.1"
                     /translation="MSAGGATIAQEPIKVGVLTIRSGPAKPIGDDILAGIETATKMYG
                     RVLNRPIELVVEESLFNAQAAVTKATKLVQQNQVASILGTSTIETLALLSVADRLRVP
                     IVTSNSGSAAITRERCNRWVFRTNAEDLMSVESLRDLVEKTPRLQQAKWFTIGHDYPW
                     SRLVASSVKGVQDLQYVGETFAPLDTTDWAPFIARARAAGATAVIMPVTLGVPLLQFI
                     QQANEFGLTGTAVLVSPIGLPDWLVEKLGSTSTNVISAGSWAAWRYEEAWPTTKEFNQ
                     AFYAMHGRVAGMQSLQSGSATLMLYNAMTKAGSVDPEAIVEALETIEVNTPVGSLKFQ
                     PNGRQALVSLFLGPYEKLDSPRYGATYAQRADPFPASKSLPKSAAEYGCKL"
     gene            1949..>2332
                     /locus_tag="VM910_13580"
     CDS             1949..>2332
                     /locus_tag="VM910_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI63597.1"
                     /translation="MNLEMPDIRGSISAKTTREWVERARALKPLLEASAAEIETSLTI
                     PNHVLDALHDAQMFRTVMPRSVGGAELDPATHAQVVAAIAEGDASVAWCIAQAACASM
                     ASAYLEPRVAQEIFGDPRAVFTFGFT"
BASE COUNT          467 a          728 c          710 g          427 t
ORIGIN      
        1 ggtatgacca acagcggcgc cacaaccagc cccagccaat agccaacgcc gacaccgtac
       61 atcacggcga tcagtgcgta gccccccatc gcatagaggg cgccatgagc aaaattgaca
      121 acccggccaa ggccgaaaac caagctaagc ccgaggctcg tcagcagcag aaaggctccg
      181 tagacgacgc cattcaagat gttgacgaat aagaaattcc aatccacgac gttgcagcct
      241 cccagccggg cagtcgaagc ggtcggctag agcttgcagc catactcggc ggcggacttg
      301 ggtaacgatt tgctcgccgg gaacgggtcc gcccgctggg catacgtggc gccgtaccgt
      361 ggactgtcga gcttctcgta ggggccgagg aacagcgaca cgagcgcctg gcggccgttg
      421 ggctgaaact tcagcgaccc caccggcgtg ttcacctcga tcgtctccag ggcttcgaca
      481 atggcctccg gatcaactga gcctgctttt gtcatggcgt tgtacaacat cagcgtcgcg
      541 ctgccggact gcagggactg cattcccgcc acccggccgt gcatcgcgta gaatgcttgg
      601 ttgaattcct tggtggtcgg ccaggcctct tcgtatcgcc aggccgccca ggaaccggca
      661 gaaatgacgt tcgtcgatgt gctccccagc ttttcgacaa gccaatcggg aagcccgatg
      721 ggcgacacga gcacagccgt tccagtcaac ccgaactcgt tcgcctgctg gatgaattgc
      781 agcagtggga cacccaaggt gaccggcatg atgacggccg tcgcgccggc cgcgcgcgcg
      841 cgcgcgataa agggcgccca atctgtggtg tcgagcggcg caaaggtctc tcccacatat
      901 tgaagatcct ggactccctt aaccgagctg gccaccaggc ggctccacgg gtaatcgtgg
      961 ccgatcgtaa accacttcgc ctgttgcagc cgtggcgtct tctcgacgag gtcccgcaac
     1021 gactcaacag acatcaggtc ttcggcgttg gtccgaaata cccagcggtt gcagcgttcg
     1081 cgagtgatgg cagccgagcc cgagttcgag gtcacgatcg gaacccgcag ccggtcagcg
     1141 acagacagaa gcgcaagcgt ttcgatggtg ctcgtgccga ggatgctggc cacctgattt
     1201 tgctggacga gcttggtcgc cttcgtcacc gcggcctgcg cgttgaacag gctctcctcg
     1261 acgacaagct ctatcggccg attgagaact cgaccgtaca tcttggtcgc cgtctcgatg
     1321 ccggcgagta tatcgtcgcc aatgggtttg gctgggccgc ttcggatcgt cagaacaccg
     1381 accttgatgg gctcttgcgc gatggtcgca ccgcccgcgc tcaagcagag gagcgatacg
     1441 aacccagtga aaagggcaga ctgcaatccc ctcatatccg gctccccaaa gtccagggta
     1501 ttgatcttgc aatgatttcc gtcccggttc aagctcacga aggggcaccc gccggaagac
     1561 aaaagagcgt tgggttgggc gcgcgcgatg ccggactgga catggtgata ggcgagctaa
     1621 cgaccgcccc ggatttgaac ctccctatga tcgtcatcga tttcgccggc gcttcgcttg
     1681 cgcctatcgg gatgaagcaa cgcgctatca catatgccga cactgccgtc ggttttcccg
     1741 gcactgacta cgcgaaagta actcgtgcgc tgagcggcaa cagttatgtc tgccgcagcc
     1801 gggaacagct caaaagcgct ctcacgcagg cagtgacgag ggaacggttc agcgtgatat
     1861 gttacgagtt tcccggcggc gcctacgacg gccgaatttg cgtggacact gcttgctgtc
     1921 ggaaccatca tgaagcacgg aggggccggt gaatctggaa atgcctgaca tccgtggctc
     1981 gatttccgcc aagaccacgc gggaatgggt cgaacgggcg cgtgccctaa aacccttgct
     2041 ggaggcgagt gccgcggaaa tcgagacgtc cctcacgata cccaaccacg tgctcgatgc
     2101 gctccatgac gcgcagatgt tccgcaccgt gatgccacgt tcggtgggcg gcgcggaact
     2161 cgatcccgcc acgcacgcgc aggtggttgc ggcgatcgcc gagggcgacg ccagcgttgc
     2221 gtggtgcatt gcccaggcgg cctgcgcctc aatggcttcc gcgtacctgg agccgcgcgt
     2281 ggcgcaggag attttcggcg atccgcgtgc agtgttcacg tttggcttca cc
//
LOCUS       DATLJT010000586         3675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1184212,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000586 DATLJT010000000
VERSION     DATLJT010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184212"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VM910_13585"
     CDS             <1..140
                     /locus_tag="VM910_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVI63598.1"
                     /translation="AGIVNERGNVLGMMPHPENHVEDIMGCTDGRGLFAGLVAHLERA
                     A"
     gene            284..439
                     /locus_tag="VM910_13590"
     CDS             284..439
                     /locus_tag="VM910_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63599.1"
                     /translation="MAYRKAHERLVDNPPWLYIVHDLNPRAMSPKVKGFVSPQSWFVD
                     LTLVSMQ"
     gene            complement(445..1344)
                     /locus_tag="VM910_13595"
     CDS             complement(445..1344)
                     /locus_tag="VM910_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI63600.1"
                     /translation="MRNYALLLALACCWSSTYPLTKIGLGSIPPISFISARSLVAAVF
                     LLAILRLRAIKIPSDGRAWKLFAFQQTINSTIPFLIITWAQQYVPASSTVVLASTTPI
                     FAFLITWGITRHEPATLLKLAGAILGLAGTAVIIGLDALGGLGQEIFAEIAILLATLS
                     FACATIFGLRLSDYDPMVVAAGSLLFGGLVLLPPAMIVDHPWTLRPTLEALIATVAMG
                     IFSSALGLMLFYMCLTRLGTLTTNAQGYLRIPIGVGLSVLLLGESVPSNLALGLILVM
                     AGVAAMTIPGRQFGRWLRLVLRR"
     gene            complement(1446..1931)
                     /locus_tag="VM910_13600"
     CDS             complement(1446..1931)
                     /locus_tag="VM910_13600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI63601.1"
                     /translation="MDDITKSAFLNRPDLHVCTEGEFSGWRTWSGDNFEINNGPFWHR
                     MEGDGSVRCVFRVEKKHLNGMCNVHGGCFMAFADYCLFAFASPVLQGPGVTVSLACEF
                     LDAAREGELVEGTGEITRAGGSLIFLRGELKSAGRPLFTFSGTIKRVKRSLAPAAALT
                     A"
     gene            2092..>3675
                     /gene="purL"
                     /locus_tag="VM910_13605"
     CDS             2092..>3675
                     /gene="purL"
                     /locus_tag="VM910_13605"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HVI63602.1"
                     /translation="MQTNEPRITPELVAAHGLKPDEYERILKLIGRTPTFTELGIFSA
                     MWNEHCSYKSSRIHLRGLPTKAPWVIQGPGENAGVIDIGDGQAVVFKMESHNHPSYIE
                     PYQGATTGVGGILRDVFTMGARPIACLNALSFGAPEHPKTRHLVSGVVAGVGGYGNSF
                     GVPTVGGQVRFHTRYDGNILVNAMAVGLADADKIFYAAASGVSMPIVYLGSKTGRDGI
                     HGASMASAEFDESSEEKRPTVQVGDPFAEKLLLEACLEIMAADCVIAIQDMGAAGLTC
                     SAVEMGAKGDLGVDLDLDAVPTRETGMSAYEMMLSESQERMLMVLKPEKEKEAEAIFR
                     KWGLDFAIVGYTTPSKRFRVKHGGDIMADLPIKELGDEAPVYDRPHVASPAQPLVHAR
                     DVTAPIGIAAALEKLIATSELCSKRWVWEQYDHIILGNTVQRPGGDAAVVRVQNGPKG
                     LALTIDVTPRYCDADPFEGGKQAVAEAWRNITAVGGKPLAITDNLNFGNPERPEIMGQ
                     FVGCLRGISEACRVLDFPVV"
BASE COUNT          741 a         1134 c         1185 g          615 t
ORIGIN      
        1 tcgccggcat cgtcaacgag cgcggcaatg tgctgggcat gatgccgcac cccgaaaacc
       61 acgtcgaaga catcatgggc tgcaccgacg ggcgcggcct gttcgcaggt ctcgtcgcgc
      121 acctggaacg cgcggcgtag gaacttttgc cccaaacaac agcacgtgag cgcaggtccg
      181 cggcgtctcg ccgaacgggt tcaacttcga acagtggaag gacgatcagt tcgacgaggc
      241 gctgaagacg atcgagacgt ccactgatcc caaagcagta tcggtggcct atcgcaaggc
      301 gcacgaacgc ctcgtcgaca atccgccctg gctctacatc gtccatgatc tcaacccgcg
      361 cgcgatgtcg ccgaaggtca agggattcgt ctcgccgcaa tcatggttcg tcgacctgac
      421 tttggtcagc atgcaatagt ccggctaccg cctgagaacg agcctgagcc accgtccgaa
      481 ctgacggccg ggaatcgtca tggcggcgac gccggccatc accaggatca ggccgagtgc
      541 aagattcgac ggcacggact cgccgagcag cagcaccgac aagccgacgc cgatgggaat
      601 gcgcaaataa ccttgcgcgt tggtggtgag cgtgccgagc cgcgtaaggc acatgtagaa
      661 cagcatcagc ccgagcgcgc tggagaagat ccccatcgcg actgtcgcga tgagcgcctc
      721 cagcgtcggc cgcaaggtcc agggatgatc gacgatcatg gcgggcggca gcaacacgag
      781 cccgccgaac agaagcgagc cggccgccac caccatcgga tcatagtccg acagtctcag
      841 tccgaagatg gtggcacagg cgaaggagag ggtggcgagc aggatcgcga tctccgcgaa
      901 aatttcctgt ccgagcccgc ccagcgcgtc gaggccgata atgacggcgg taccggcgag
      961 accgaggatg gcacccgcaa gcttcagcag cgtggcgggc tcatggcgcg tgatgcccca
     1021 ggtgatcagg aaggcgaaga tcggcgtggt cgaggcgagc acgaccgtgc ttgaggccgg
     1081 cacgtattgt tgcgcccagg tgatgatcag gaacgggatc gtcgaattga tggtctgctg
     1141 gaaggcgaac agcttccagg ccctgccgtc actcggaatc ttgattgcgc gtagccgcaa
     1201 aatcgcgagc agaaaaaccg ccgcgacaag cgagcgggcg gagatgaagc tgatcggtgg
     1261 gatcgagcct agaccgatct tggtcagcgg ataggtcgag ctccagcagc aggcgagcgc
     1321 gagcagcagc gcgtagttgc gcaagctatg tcgggccgtc ccggctgacg gagcggccgt
     1381 tgtcgcgagg tccgttgcat catcccgtgc tgccgccaag tcgtcgtcct gccgcggtcg
     1441 tggatctagg cggtgagggc agccgccggc gcaaggctgc gcttcacgcg cttgatggtg
     1501 ccggaaaaag taaagagggg gcgcccggcg gatttgagct cgccgcgcag gaaaatcagc
     1561 gaaccgccgg ctcgggtgat ttcgcccgtt ccttcgacaa gctcgccctc gcgcgcagca
     1621 tcgagaaatt cgcaggcgag ggagaccgtg acacccggcc cctgcagcac cggcgaggcg
     1681 aaagcaaaca ggcaataatc ggcgaaagcc atgaagcagc cgccatggac attgcacatg
     1741 ccgttcaagt gctttttctc gacccggaag acgcagcgga ccgagccgtc accctccatc
     1801 cggtgccaga acggcccatt attgatctcg aaattgtcgc cgctccaggt gcgccagccc
     1861 gaaaattcgc cctccgtgca gacatgcagg tcggggcggt tcaaaaatgc cgatttggta
     1921 atgtcgtcca cgcgataggc cttcaatttg ggggtacaag tccttaaatc cgatcctcaa
     1981 gcaaggtgta aacccgctct ctcgcatggc gctcctgcaa agctctggac agcagggaaa
     2041 cgggccctaa aaggcctttt ccgcctcctc cgatctccct aataacggtt tatgcagacg
     2101 aacgaaccca ggattacccc cgagctcgtc gccgcccacg ggctgaagcc ggacgagtat
     2161 gagcgcatcc tcaaattgat cgggcggacg ccgacattca ctgagctcgg gattttctcg
     2221 gcgatgtgga acgagcactg ctcgtacaaa tcttcccgca tccatctgcg cggcttgccc
     2281 accaaggcgc cctgggtaat ccaggggccg ggcgagaatg ccggggtgat cgatatcggc
     2341 gacggccagg cggtggtttt caagatggag agccacaacc acccgagcta catcgagccg
     2401 tatcagggtg cgaccaccgg cgtcggcggc attttgcgcg acgtcttcac catgggcgcg
     2461 cgtcccattg cctgcctcaa cgcgctctcc ttcggcgcgc cggagcatcc caaaacgcgg
     2521 cacctcgtct ccggcgtggt cgccggcgtc ggcggctacg gcaattcgtt tggcgtgccc
     2581 accgttggcg ggcaggtgcg tttccacacc cgctacgacg gcaacatcct ggtcaatgca
     2641 atggcggtgg ggctcgccga cgccgacaag atcttctacg cggcggcctc cggcgtgagc
     2701 atgccgatcg tctatctggg ctccaagacc gggcgcgacg gcattcacgg cgcctcgatg
     2761 gcatcggctg aattcgacga gtcctcggag gaaaaacgcc cgaccgtgca ggtcggcgat
     2821 ccctttgcgg aaaagcttct gctcgaggca tgcctcgaga tcatggcggc cgattgcgtg
     2881 atcgcgatcc aggatatggg cgcggcgggc ctgacttgct cggcggtcga gatgggggcc
     2941 aaaggcgatc tcggcgtcga tctcgatctc gacgccgtgc cgacacgcga aaccggcatg
     3001 agcgcctatg agatgatgct gtcggaaagc caggagcgca tgctcatggt gctcaagccc
     3061 gagaaggaaa aagaggcgga agctatcttc cgcaagtggg gcctcgactt tgcgatcgtc
     3121 ggctacacca cgccgagcaa gcgctttcgc gtcaagcatg gcggcgacat catggccgac
     3181 ctgccgatca aggaactagg cgacgaggcg ccggtctatg atcggccaca tgtggcttcg
     3241 ccggctcagc cgctcgtgca cgcgcgcgat gtgactgcgc cgatcggcat tgctgcggcc
     3301 ctggagaagc tgatcgcgac gtccgaattg tgctccaagc gctgggtgtg ggagcagtat
     3361 gatcacatca ttctcggcaa cacggtgcag cgtcccggcg gcgatgccgc agttgtccgc
     3421 gtgcaaaacg ggccgaaggg gctcgcgctc accatcgatg tcacgccgcg ctattgcgac
     3481 gccgatccgt tcgagggcgg caagcaggcg gttgcggaag cctggcgcaa tataacggcc
     3541 gtcggcggca agccgctggc gatcaccgac aatctcaatt tcggcaatcc ggagcggccc
     3601 gagatcatgg gccagttcgt cggctgcctg agggggattt cggaggcctg ccgggtgctg
     3661 gatttccccg tcgtg
//
LOCUS       DATLJT010000587         5749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2792937,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000587 DATLJT010000000
VERSION     DATLJT010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2792937"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2118)
                     /locus_tag="VM910_13610"
     CDS             complement(<1..2118)
                     /locus_tag="VM910_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVI63603.1"
                     /translation="MYKKGIEGFPYYLGLASLSEVATRSDRVCVLNILGGESRQVTPV
                     SHAFSGGNVVFGTSPGRRGQVLPTPAGDIPVYNNVREGLDDGLTFNTGVVYLPPSGVR
                     DGVAELVRVNPQLEKIVMITEKVSVHDAREIRALGQANGIDILGANCLGVADSWNRVR
                     IGGALGGDNPEEVLLKGSVAIFSNSGGFTTTIAQYLSTEGWGTSTLISSGKDVYIHYA
                     ARDFALAFNNDIRSEAAVLYAEPGGYYERGVEFGKPVIACIVGRWKSKLTRAVGHAGA
                     MAGSGDTAEDKERWFMEGFGVDGIFAPDRPVVSAKGAVVTNIAHIPLALTAVMKLNNA
                     RPDFAPRGSLSLKPWIANDQGIKLPAHLVLPTVEAIPPYNDQIKALARQIGAVIPRQN
                     MKDKSGATVMDPSTQVTSVHGHSVLDLAVLPLEANFALPLVHEIASSNDRAMLDIAVA
                     AETNLVGDPALMAAEASREAGNSPNTIMAAAAAIIGPKRVERALCCATRLIDLFAHSG
                     LVDARDETFDSSKIRVDEGTRVLFSATAAEANDPRPEAMLKAVRDRGGKSLFLRFVES
                     LGAQPSRDTILAAISTTIAWGPLMRKRISRLTAETLPWYLRLYGVMIGASIPARHHQH
                     GSLCGISREERFSRWSMADVLFLALTGKKPTEAEARPLQILIGLLISNGPGSISAQGA
                     KGAVAADGPQTPSRVQINKAMVGF"
     gene            complement(2143..3423)
                     /locus_tag="VM910_13615"
     CDS             complement(2143..3423)
                     /locus_tag="VM910_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HVI63604.1"
                     /translation="MNVTGMLYGSRLLAHVDFPTSEVLGPGASESEIQDLINRHKLIF
                     IKPLFKGGVGKKGKSGLIGKASDLKTALAEKERLFFAEHHHGHVTAKANGVTFESGVP
                     AQHEVYLAITDSTEFRAPTMTLTHMGGVDIEELDKKQVARVPFEALTGLKAFVVANAL
                     TDIGAPKEIISPLVQHLPKLWELVHHYGMTTLELNPIRMRPGREGRLTPVACDFKCGF
                     DRDDPRWERLDLPDHLFTADSSDFEQEVNQLRTHQGQSDVFVINPAGTILAPTFGGGA
                     NSLVTQVLGDDAIISSDFGGNPPYEKMKEVARICYKHWLKQTNVLFIIGGKSNNTDIY
                     ETFRAMGDALREHFSRHGPSPLYVVVGRGGPNLVRGMGALAETCDSLNLPYRFFGFDS
                     AISEVVNYTKKVDEWMKAGGRKEIAAHLGLQQVH"
     gene            complement(3436..4791)
                     /locus_tag="VM910_13620"
     CDS             complement(3436..4791)
                     /locus_tag="VM910_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HVI63605.1"
                     /translation="MQQSVSVSASPGMLRPRWWPEGWWKIVDLRIGIIPIPIYVILIA
                     LLIGFVFTGDIKGEISVMIAVLVIGGFTCAEIGKRIPVLRNIGAAAIFATFVPSALAY
                     YKLLPPQIEKSVIEFTKYTNFLYLFIACIIVGSILGMDRKVLVQGFLKIFVPLAIGSI
                     AAGIVGTLVGTLLGLGASHTFFFVVIPIMAGGVGEGAIPLSVGYSEILGEPQGDIFAQ
                     VLPPVMLGSLTAILLAGTLNYVGKRYPNLTGEGRLQPGEHDEMDTAKQGAEDEAGAPI
                     DVGTVAAAGLTAITLYLLGIMCHRLFGLPAPVAMLFLAVLAKLTHGVSPHLQQGGFVV
                     YKFFSTAVTYPLLFAIGVSMTPWDKLMAAFNLPNLITIVSTVATLMATGALVGRWLNM
                     YPIEAAIINACHSGQGGTGDVAILTAANRMQLMPFAQIATRIGGAITVTLVLILLSRL
                     V"
     gene            complement(4847..>5749)
                     /locus_tag="VM910_13625"
     CDS             complement(4847..>5749)
                     /locus_tag="VM910_13625"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63606.1"
                     /translation="IAISDFEGGGQSFDALLNSSVDIAAGAYEHVLRAKAMGHDIRAV
                     IELGRFPGVVLAVGKNRPFKSYADLKGLKIGVTAPNSSSHFFVLYLMARAGLAPTDVS
                     FVGVGGGERAVNAMAAAEIDAISNLDPLITRLQQDGTVRVVADTRFPRVNYEILGGTN
                     PAAVLYAKQEFIAANPATMQALVNAFYKTLRWIATATTDEIVNSVPPDYFLGDRAIYV
                     KALKANLLVYSRTGIITRQGMDSALKMMATFDPDLKGKKIDLQTTFDARFIKRATVLF
                     DDPTNSDLETDDRRPEIDLNRFQD"
BASE COUNT         1139 a         1718 c         1755 g         1137 t
ORIGIN      
        1 gaagccgacc attgccttgt tgatctgaac gcggctcggc gtctgcggcc cgtcagcggc
       61 aactgcgcct tttgcgcctt gcgccgatat cgaacctggg ccattcgaaa tcaggaggcc
      121 aatgagaatc tgcagcggcc tcgcttcggc ctccgttggt ttcttcccgg tcaaagccag
      181 gaagagcaca tccgccatgc tccagcggct gaatcgttcc tcgcgcgaaa tgccacacag
      241 actgccgtgc tggtggtgcc tagcggggat cgaggcgccg atcatcacgc cgtaaagacg
      301 cagataccag ggcagtgtct ccgcggtgag gcgcgagatc cgtttgcgca ttaacgggcc
      361 ccaggcgatg gtggtcgaga tcgccgcgag gatagtgtcg cgcgacggct gcgcgccgag
      421 cgattctacg aatctcagga acagcgattt gccgccgcga tcgcggaccg ctttcagcat
      481 ggcctcggga cggggatcgt tggcctcggc ggcggtggcg gaaaagagca cgcgcgtgcc
      541 ctcgtcgacc ctgatcttgc tggaatcgaa ggtctcgtca cgcgcatcga ccaagccaga
      601 atgggcaaac aggtcgatca gccgggtcgc gcagcaaagc gcccgctcga cgcgctttgg
      661 accgatgatc gccgccgccg ccgccattat ggtgttcggg gagttgccgg cttcgcgcga
      721 cgcctccgcc gccatcaggg ccggatcgcc tacgagatta gtctcggcgg caaccgcaat
      781 gtcgagcatc gcgcggtcat tcgaactggc gatctcgtgc acgagcggca acgcgaaatt
      841 ggcttcgagc ggcagaaccg cgagatcgag cacggagtgg ccgtgcacgc tcgtcacctg
      901 cgtgctcggg tccatgaccg ttgcacccga cttgtccttc atgttctggc gtggaatgac
      961 cgcgccaatc tggcgggcaa gggcctttat ctggtcattg tatggaggga ttgcttcaac
     1021 cgtcggcaac acgaggtgcg caggcagctt gataccctgg tcgttcgcaa tccagggctt
     1081 gagcgaaaga ctaccgcgag gcgcgaaatc cggccgcgca ttgttgagct tcatgaccgc
     1141 ggtgagcgca agcgggatat gggcgatgtt ggtcacgaca gcacctttgg ccgacaccac
     1201 cggacgatcg ggcgcgaaga tcccatccac gccgaagcct tccatgaacc agcgttcctt
     1261 gtcctcggcg gtgtcgcccg agccggccat cgcgccggcg tgcccgaccg ctcgtgtcag
     1321 cttcgacttc caccgtccga cgatgcaagc gatgaccggc ttaccaaatt caaccccccg
     1381 ctcgtagtaa ccgccaggct cggcgtagag caccgcggcc tcgctgcgta tgtcattgtt
     1441 gaatgcaagg gcgaagtcgc gcgctgcata gtggatatag acgtcttttc ccgacgagat
     1501 gagcgtgctc gtcccccaac cttccgtgct gagatattgc gcgatggtcg ttgtgaagcc
     1561 gccggaattc gaaaagatag caaccgatcc cttgagcaat acttcctcgg ggttatcgcc
     1621 gcccagggcg ccgccgatcc gcacgcgatt ccaggagtct gcaacgccca ggcaatttgc
     1681 ccccaagatg tcgatgccgt ttgcctggcc cagcgctcgg atctcgcgtg cgtcatgcac
     1741 cgagaccttc tcggtaatca tcacgatttt ctcgagctgt gggttgacgc gcacgagctc
     1801 ggcaacgccg tcgcgcaccc cggacggggg aaggtacacg acgccggtat tgaacgtgag
     1861 cccgtcatcg agaccttcgc ggacattgtt gtagacgggg atatcgccgg ctggggtcgg
     1921 cagcacctgg ccgcgtcgtc ctggcgacgt cccgaataca acattaccgc cggagaatgc
     1981 gtggcttacc ggggtcacct gtcgggactc tccgccgagg atgttaagca cgcacactcg
     2041 atcactgcgc gtggccacct cgctcagtga ggcgagcccg agataatagg gaaacccctc
     2101 gatgcccttc ttgtacatgc ccgacttctt tctggccgca gctcagtgga cttgctgcag
     2161 acctaaatgc gcggcgatct ccttgcggcc gcccgccttc atccattcgt cgaccttctt
     2221 ggtgtagttc acgacttcgg agatcgcgct gtcgaaaccg aagaaacggt agggcaggtt
     2281 gagtgaatcg caggtctcgg cgagcgcccc catgccgcgc accagattgg gtccgccgcg
     2341 gccaaccacg acatagagcg gcgacgggcc atgccggctg aaatgctcgc gcagggcatc
     2401 gcccatcgcg cggaaggtct catatatgtc ggtgttgttc gacttgccgc cgatgatgaa
     2461 cagcacgttg gtctgcttga gccagtgctt gtagcagatg cgcgccacct ccttcatttt
     2521 ttcgtagggg ggattgccgc cgaaatccga tgagatgatg gcatcgtcgc caagcacttg
     2581 agtcaccagc gagttcgctc cgccgccgaa cgtcggcgcg agaatggtgc cggcaggatt
     2641 gatgacgaat acgtccgatt gaccctgatg ggtgcgcagc tgattgacct cctgttcgaa
     2701 gtccgaactg tcggcggtga acagatgatc tggcagatcg agtcgttccc agcggggatc
     2761 gtcgcgatcg aaaccgcact tgaagtcgca cgcgactggc gtgaggcgtc cttcgcgacc
     2821 tggacgcatg cggatcgggt tgagctccag cgtggtcatg ccgtagtggt ggacgagctc
     2881 ccaaagcttg ggcaaatgct ggaccagcgg cgagatgatc tccttcggcg cgccgatgtc
     2941 ggtgagcgcg ttggcaacga cgaaagcctt caggccagtt agggcttcga acggaacccg
     3001 cgcgacttgc ttcttgtcca gctcctcgat gtcgacgccg cccatatggg tgagcgtcat
     3061 ggtgggggcg cggaattccg tggaatcggt gatggctaaa tagacctcat gctgggccgg
     3121 cacgccgctt tcaaaggtca cgccgttcgc cttcgcggtc acatggccgt ggtgatgttc
     3181 cgcgaaaaac aggcgctctt tctccgccag ggccgtcttg agatcgctcg ccttgccgat
     3241 cagcccggac ttgcccttct tgccgacacc acctttgaac aacggtttga tgaagatgag
     3301 cttatgccgg ttgatgagat cctggatctc gctctcgctt gctcccgggc cgaggacttc
     3361 ggaggtagga aagtccacgt gagccaacag gcgtgaacca tagagcatgc cggtgacgtt
     3421 catcttactt ctcgcctaaa ccaatcgcga tagtagtatc agcacaaggg taacggtgat
     3481 ggccccgcca atgcgggtcg cgatttgcgc aaatggcatg agctgcatcc ggttcgccgc
     3541 cgtgagaatc gcaacatcgc cggtgccgcc ttgcccgctg tggcaggcat taataatggc
     3601 cgcttcgatg ggatacatat taagccagcg cccaaccaac gctccggtgg ccatcagtgt
     3661 cgccaccgtg ctgacgatcg tgatgaggtt cggcagattg aacgcagcca tcagcttgtc
     3721 ccacggggtc atggacacgc cgatcgcgaa caacagcgga taagtgacgg cggtcgagaa
     3781 aaatttgtaa acgacgaatc cgccctgctg caggtggggt gagaccccgt gcgtcagctt
     3841 ggctagtacc gcaaggaaca gcatggcgac cggcgccggc agtccgaaaa ggcgatggca
     3901 catgatgccc aggagataga gcgttattgc cgttaggccc gcggctgcga cggtgccgac
     3961 atcgatcggg gcgccagctt cgtcctcggc gccctgtttg gcagtatcca tttcgtcgtg
     4021 ctcgcccggc tgaagtcgac cttcgcccgt cagattcgga taccgcttgc cgacatagtt
     4081 gagcgtgccg gcaaggagga tcgcggtgag gctgccgagc atcactggcg gcagcacctg
     4141 ggcgaatatg tcgccctggg gctcgccgag aatttccgaa tacccgacgg agagcgggat
     4201 cgcgccctcg ccaacaccac cggccatgat gggaattaca acgaagaaga acgtgtgaga
     4261 ggcgccgagc ccaagcaagg tgccaaccag cgtgccgacg atgccggcgg cgatcgagcc
     4321 aatcgccagc ggtacgaata ttttcaggaa tccctgaacc agaaccttgc ggtccatgcc
     4381 caggatgctg ccgacgatga tgcaggcgat gaacaggtaa aggaagttgg tatacttggt
     4441 gaattcgatg accgattttt cgatttgcgg cggcagcaac ttgtaatagg cgagcgctga
     4501 cgggacgaaa gtggcaaaga ttgcggccgc cccgatattg cgaagcactg gaatacgctt
     4561 accgatttcc gcgcaggtga agccgccgat caccagaacg gcaatcatga cggagatttc
     4621 gcccttgatg tcaccagtga agacgaagcc aatcagtagc gcgatgagaa tgacataaat
     4681 cgggatcggg atgatcccga tccgcaagtc gacgatcttc caccaccctt ccggccacca
     4741 acgcgggcgc aacatacctg gcgaagccga gaccgagacg ctctgctgca tggtccgctc
     4801 caatttgcgc ttcggcgaga aggtgcctgc tatcgtgttg ccatcatcag tcttgaaacc
     4861 tgttgaggtc gatctcggga cggcgatcgt ccgtctccag gtcgctgttt gtcggatcgt
     4921 cgaataggac tgtcgcacgc ttgatgaacc gagcgtcaaa cgtcgtttgc aggtcgatct
     4981 ttttgccttt gagatcggga tcgaacgtcg ccatcatctt aagagcgctg tccattccct
     5041 ggcgggtgat gatccccgtt cgcgaataga cgagcaggtt ggctttcagc gctttaacat
     5101 agatcgctcg atcgccgaga aagtagtcgg gtggcacgct gttgactatc tcatccgtcg
     5161 ttgcggtcgc gatccatcgc agcgtcttat aaaacgcgtt gaccagggcc tgcatcgtcg
     5221 ccgggttggc ggcaataaac tcctgcttgg catagagcac ggccgccggg ttggtgccac
     5281 cgaggatctc gtaatttact cgcgggaagc gcgtatcggc gacgactctt accgttccat
     5341 cctgctgcag acgggtaatc aacggatcga ggttcgagat tgcgtcgatc tccgccgccg
     5401 ccatcgcatt gacggcccgt tctccgccgc ccacgcccac gaacgaaaca tcagtcggcg
     5461 caagaccagc cctcgccatg aggtagagca caaagaagtg gctggaagag ttcggggcag
     5521 tcacaccgat cttgaggcct ttgagatcgg catatgactt gaaaggccgg ttcttgccga
     5581 cggcgagcac cacgcctggg aatcggccaa gctcgatcac ggcgcggatg tcgtgtccca
     5641 tggctttcgc tcgtaataca tgctcgtacg cacctgctgc gatatcgaca gagctgttga
     5701 gcagggcatc gaacgattgc cccccgcctt caaaatcgct gatcgcgat
//
LOCUS       DATLJT010000588         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1873142,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000588 DATLJT010000000
VERSION     DATLJT010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873142"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VM910_13630"
     CDS             <1..459
                     /locus_tag="VM910_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI63607.1"
                     /translation="VQTFPTSDGWLFIMCMTDKFWGELLAAIDRTDLASDPRFATQAL
                     RQENRDALMGIIDSELKRESTTFWLDRLNGVLPVAPVLELSQALDSPFLKVTDMIHTI
                     AHPAKPGMRVLANPIKINGERLEQTPCSPYGADTASYVGEMCAAEPASSQ"
     gene            456..1583
                     /locus_tag="VM910_13635"
     CDS             456..1583
                     /locus_tag="VM910_13635"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI63608.1"
                     /translation="MKLEGIRVIDLSVFLPGPYLTLAMADHGAEVIKIEPPGEGDPGR
                     HIGLSDGPSTVFFRNLNRGKKSVELDLKDPAQREALLSLAETADVFIESYRPGTVDRL
                     GVGYEMVRARNPRIVYCSISAFGQDSEWRGRPAHDLALEAESGLLSMTLGNDGKPAMP
                     GIPVADVLAGLQGLSGVLMALLRRDKTGAGDYLDISMHDVTVGGMLNILGPTFAEDRQ
                     PVPAHERTTGGAAFYRPYETQDGRYLVLAGQEPKFIHNLLRAFGRPELAELCLRGPGP
                     HQRTVIEFLSAEFRKRPLADWQAHLATLDVCYGRINTLPEAFAHPNLLTRGMILYDET
                     KRRHVGPPIHFRHEPARPVLREPALGEHTASILGKCVSTSE"
     gene            1588..2091
                     /locus_tag="VM910_13640"
     CDS             1588..2091
                     /locus_tag="VM910_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVI63609.1"
                     /translation="MQGHDQIGPQRYRESFGRYYDDFVVGDVYEHRPGRTITESDNTW
                     FTLITMNTHPLHFDKAYAAKSEFKRPLVNSCLTLSMVVGMSVSDISQKAIGNLGWNDI
                     KLTAPVFVGDTIYAESEVLAKRESSSRPGQGLVTVRTIGKKADGTQFMSFERTALIAK
                     RGYGIED"
     gene            2075..2884
                     /locus_tag="VM910_13645"
     CDS             2075..2884
                     /locus_tag="VM910_13645"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI63610.1"
                     /translation="MASKIDIKARRANSPKGASQMRAPVSVKSDEPLYLQVVRVLKDA
                     IVSGMYPVGSQLPTEEELCEQFSVSRYTVREALRRLREDSLVSSRQGAGTIVVPPRPT
                     DSFVHEVMSINDLVAFATGVRFAIDTIEMIEIDDKLASRTGIPPGEKWLAVRGFRHTE
                     GSDLPVCWTEVYINREFAAVGRLLQRHRGPIFHLIEDLFGQSIVEVHQEIAAALVSPA
                     LAAGLKVKAGATALEVQRTYTLATGKIAQVAINTHPASRFRHSMTMRRVKG"
     gene            3498..3701
                     /locus_tag="VM910_13650"
     CDS             3498..3701
                     /locus_tag="VM910_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63611.1"
                     /translation="MKLIVDGYVKLSARKALDDLKAHRCRLATELKSINGPFDLSSSI
                     KQLEKEIEVIEEGLARLDTCAVA"
     gene            complement(3969..>4107)
                     /locus_tag="VM910_13655"
     CDS             complement(3969..>4107)
                     /locus_tag="VM910_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVI63612.1"
                     /translation="EHGVGQKKMKYLETEHGAPAVELMRTLKRAIDPLNIMNPGKVVA
                     V"
BASE COUNT          803 a         1260 c         1229 g          815 t
ORIGIN      
        1 gtgcagacgt tcccgacctc ggacggctgg ctgttcatca tgtgcatgac cgacaagttc
       61 tggggcgagc tgctcgcagc gatcgaccgg accgatcttg cctccgatcc gcgctttgcg
      121 acgcaggcct tgcgccagga aaatcgcgac gcgctgatgg gcatcatcga cagcgaattg
      181 aagcgcgagt ctaccacttt ctggctcgac aggctgaacg gcgttctgcc cgtcgccccc
      241 gtgcttgagc tttcgcaggc gctggacagt ccatttctga aggtgacgga catgatccac
      301 accatcgcgc acccggccaa gccggggatg cgggtgcttg ccaacccgat aaaaatcaac
      361 ggtgagcgcc tcgagcagac gccgtgctcg ccctatggcg ccgacaccgc ctcctatgtg
      421 ggcgagatgt gcgccgccga gcccgcgagt tcgcaatgaa gctcgaaggc atacgcgtca
      481 tcgatctttc ggtgtttctg ccgggaccgt atctgacgct ggcgatggcc gatcacggcg
      541 ccgaggtgat caagatcgag ccaccgggcg aaggcgatcc cggccggcat atcggcctct
      601 cggacgggcc gagtacggtg ttcttccgca atctcaatcg cggcaagaaa agcgtcgagc
      661 tggatctgaa ggatcccgcg caacgcgagg cgctgttgtc gctcgcagag accgcggacg
      721 tcttcatcga gtcttatcgt ccgggcactg tcgatcgcct cggcgtgggg tatgagatgg
      781 tgcgcgcacg caatccccgc atcgtctatt gctcgatcag cgcgttcggg caggacagcg
      841 aatggcgagg tcgcccggcc cacgatctgg cgctggaagc cgagagcggg ctactcagca
      901 tgacgcttgg aaatgacggc aagcccgcga tgcccggcat tcccgtcgcc gacgtgctgg
      961 ccggactaca gggcctgtcg ggcgtgctga tggcgctgtt acgccgggac aagaccggcg
     1021 ccggcgacta tctcgatatc agcatgcacg atgtcacggt tggcgggatg ctcaacatcc
     1081 tgggaccaac ctttgccgag gatcgccaac cggtccccgc ccatgaacgc accactggcg
     1141 gcgcggcgtt ctatcgtccc tatgagacgc aggacggacg atatctcgta cttgcagggc
     1201 aggaaccgaa attcatccac aatctgctgc gcgcgttcgg ccgccccgag cttgccgaac
     1261 tatgcctgcg cggccccggt ccgcatcagc ggaccgttat cgaattcctc agcgccgaat
     1321 tccggaagcg gccgctcgcg gactggcaag cccatctcgc gaccctcgac gtctgttatg
     1381 gacgcatcaa cacacttcct gaagcgttcg cgcatccgaa tctgctcacg cgcggaatga
     1441 ttctctacga tgagacgaag cgccgtcacg tagggccgcc gatccacttc cgtcatgagc
     1501 cggcgcggcc ggtattgcga gagcccgcct tgggcgagca cacggcgtcg atcctgggca
     1561 aatgcgtttc aacttcggag tgaggcgatg caaggccacg atcaaatcgg tccccaacgc
     1621 taccgcgaga gtttcggcag atactatgac gacttcgttg tcggcgacgt ctacgagcac
     1681 cgcccgggcc gcaccatcac cgaaagcgac aatacctggt tcacgttgat cacgatgaac
     1741 acccatcccc tgcatttcga taaggcttac gcggcaaagt cggaattcaa gcgaccgctc
     1801 gtcaatagct gcctgacact gtcgatggtg gtcggcatga gtgtcagcga catcagtcag
     1861 aaggctatcg gcaatctcgg atggaacgac atcaagctta ccgcgccggt tttcgtgggc
     1921 gacacgatct atgccgaatc ggaagtgctg gcgaagcgcg agtcgtcctc gcggcccggg
     1981 caaggccttg tcaccgtgcg cacgattggc aagaaggcgg acgggacgca gttcatgagc
     2041 ttcgagcgga cggcgctgat cgccaagaga ggctatggca tcgaagattg acatcaaggc
     2101 acgccgagcc aactcgccga agggcgcgtc ccaaatgcgc gcgcccgtca gcgtcaaatc
     2161 ggacgaaccg ctctatctcc aggtcgtccg cgtcctgaag gacgcgatcg tcagcggcat
     2221 gtatccggtg ggttcgcaac tgccgaccga ggaggaattg tgcgagcagt tctcggtcag
     2281 ccgctacacg gtccgcgaag ccttaagacg cctgcgtgag gacagtctcg tctcctcccg
     2341 gcaaggcgcg ggcacgatcg tcgtcccgcc gcgacccact gactcctttg tccatgaggt
     2401 catgtcaatc aatgacctcg tcgccttcgc gacgggcgtc cgttttgcga tcgacaccat
     2461 cgagatgatc gagatcgacg ataaactcgc gtcccggacc gggattccgc ctggcgaaaa
     2521 gtggcttgct gtccgcggtt tccgtcatac cgagggcagc gaccttccgg tttgctggac
     2581 cgaagtttac atcaatcggg aatttgcagc ggtcgggcgg ctgttgcagc gtcatcgtgg
     2641 gccgatcttt catctgatcg aagacctgtt cggtcagagc atcgtcgagg tccaccagga
     2701 aatcgcggcc gcactggttt cgccggcgct tgcggccgga ctcaaggtca aggccggagc
     2761 cacggcgctc gaggtgcagc gaacttacac gttggccaca ggcaagatcg cgcaggtcgc
     2821 tatcaatacc catcccgcat cccgcttccg tcattcgatg acgatgcggc gcgtaaaggg
     2881 ctgacattcg aggccggcct cgcgcgcaag gtcatcttct acgtcgcttc tggcccctgg
     2941 tcccgtgcgc gggcagcgaa gtgaccggcc ttaaccggcg gctcacttat tgttccttgt
     3001 gaatcgcctg gttcggcccg aataatttat gggcttgtcc gagccgtttt ccaatttcac
     3061 gcgctcaaga accttctcaa ggtgcgcgat ctcgcgctta aggacgccga tttcgtgctt
     3121 gcttacatct tgattgccgc gccgaagtgt caattcgccc gtctcgagac ggtcgagcaa
     3181 atcgcgaaac gttgccaatt cgtcggcgcg atcgggactg ttcatgctcc aagtatgcgc
     3241 ccgaattgag ctgattccta gttggaagac cttctgccac tgctcctgat ctcgcggagc
     3301 cgaggtgggg tgacggtgcg gatcgcatgg gaaacgcaac cgcaagtggc tatggttgca
     3361 tcgcaagcgg ccgcggcgcg gcggtaatta gatcgccttg cgccgcaccg gaatgacaga
     3421 gaaactaggc ccgcgctggg caaccggcgg attcttattt tattgtttaa agcgagtttt
     3481 gaaagatgtc agaagcgatg aaattgatcg tcgacgggta tgttaaattg agcgctcgca
     3541 aagcgttgga cgatctgaag gcccaccgct gccgtctggc gaccgaactc aagtcaatca
     3601 acggcccctt cgacttaagc tcctcgatca aacaactcga gaaggaaatc gaggtcatcg
     3661 aggaggggct tgcgagactg gatacatgcg cggtggcctg atcggcgcaa ggggttcgcg
     3721 tcccgtctat cgttctacgg ctcgaacgcg cgttcgaact ggcgcttgct gaccatactg
     3781 aaatgctcct ctctcggagg agggccgatc gtggcccagg aagccacgcc aataacaatg
     3841 gctgccaaaa tcgtggccgc gacttctcta aaattcattt ctcgattgcc ccggccgaaa
     3901 tcctgcgagc ttgtcgccgc gcgtggcggc cgtcctgttc agacctgaca tctgacgagc
     3961 agatcacgtc agactgcaac caccttcccc ggattcatga tgttcagcgg atcgatcgcg
     4021 cgcttcagcg tgcgcataag ctccactgcc ggcgcgccgt gttcggtttc gagatatttc
     4081 atctttttct gtccgacgcc atgctcg
//
LOCUS       DATLJT010000589         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2103014,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000589 DATLJT010000000
VERSION     DATLJT010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103014"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM910_13660"
     CDS             <1..315
                     /locus_tag="VM910_13660"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI63613.1"
                     /translation="ASAHIPAAVMLIGGVGWMAGPACIIPRQSVELYNLCKRQRWEDA
                     MALQRRLWRVNEAFARFNLAACIKAGLAIQGYDVGDPVPPQAALNAEERKAIEAVLGD
                     LG"
     gene            547..1140
                     /locus_tag="VM910_13665"
     CDS             547..1140
                     /locus_tag="VM910_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVI63614.1"
                     /translation="MRRFLILLATLSCLSLSNCGYNTIQSNDEQIKANWSEVVNQYQR
                     RSDLVPNLVNSVKGFAQQEKDVLLGVTNARAKVGSVQATPEVLNDPAAFQKFQQAQGE
                     LSSALSRLLVVTENYPQLKSDALFRDLMSQLEGTENRITVARNRYIKSVQDYNVTIRT
                     FPTNLTAMLFGYKEKPNFTVENEKEISSAPKVDFGTK"
     gene            1165..2073
                     /locus_tag="VM910_13670"
     CDS             1165..2073
                     /locus_tag="VM910_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HVI63615.1"
                     /translation="MSRAGIFFVALLLCWVSIAAADVAVPPLTGRIVDLTGTLSADTI
                     ARESQRLKDLQTRKGSQVAVLIVPTTQPESIEQYSIRVADTWKVGRKGIDDGALLVVA
                     KNDHKLRIEVGYGLEGALTDVTARRIIDEVIVPRFKSGDFDGGITAGITRLVSVIEGE
                     PLPVPQPEASHGIDEDWVSFIFSPFGLLMFAAIAALLRGLLGRLLGSGATAGVIGVLA
                     WFVGGSIAVAAILGFAAFFLTLFGDSMFAASQNASRTAGRGGYTGGGWSGGGWSGGGG
                     GWSGGSGGGFSGGGGGFGGGGASGSW"
     gene            2076..2573
                     /locus_tag="VM910_13675"
     CDS             2076..2573
                     /locus_tag="VM910_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HVI63616.1"
                     /translation="MGIKRVGKHLLEHRWRARRLFPPPVLARIEAAIKAGETTHSGQL
                     RFVVEGALDGAPLLHDQPARERALDIFSHLRIWDTAHNNGVLIYVLLADRKVEIIADR
                     GIDASVGAEGWARICHDMDMEFRAGRFEAGVLKGISEVSHELARYFPARGPHPNELPD
                     KPVVI"
     gene            2736..>4048
                     /locus_tag="VM910_13680"
     CDS             2736..>4048
                     /locus_tag="VM910_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63617.1"
                     /translation="MPKTGIKPGTSRRTLLKNSAAAAAVLSLDWSRARAEAETVRMGV
                     MYDLTGPFAAGGSVAFSVGTQIAIDLVNEKGGVAGKYKVDPVNVDSQSKPDVAINEGE
                     RLISQEKVDVINGVFASAQAVPLAARVEQHKKILWITTAVSTAVFKDKNLRYVFRGQI
                     HSDQYGQAFAGFLSEHAKAKLGIEPKDVKVALIHEDGPYGVGVAAADEAYAKEAGLNV
                     VLKEGYSASAPDLSVLVTKIKRARVDVISHAGYNPDITLFLRQAREGGLRFKMLFGAG
                     AGYSQLDKLRATFGPDIDNFCNIDPVPAQLLDPAKLAPGVGDLIKTMVERFKAKTGAT
                     EVPPHCSMGFNQTWVLLNNVLPVAKEKYGGFDPEAIRKAALDVDIPPGGTIQGYGVKF
                     YPPDTPMSGQNERSTPVVMQNAGEHISVVWPTNIRTQEPVFPLPKS"
BASE COUNT          757 a         1271 c         1284 g          736 t
ORIGIN      
        1 gcgtccgccc atattccggc cgccgtgatg ctgatcggcg gcgtcggctg gatggcggga
       61 cctgcctgca tcatccccag gcaaagcgtc gaactctaca atctctgcaa gcggcaacgc
      121 tgggaggatg cgatggcgct ccagcgcagg ctgtggcgcg tcaacgaggc gtttgcccgt
      181 ttcaacctgg ccgcctgcat caaggccggg ctcgccatcc agggctatga tgtcggcgat
      241 cccgtgccgc cgcaggccgc gctcaacgcc gaggaacgta aggccataga ggcggtgctc
      301 ggggatttgg ggtgaacgcg cataacattt cggcggtcgt cgcggccggg cttgatgcca
      361 tcccgaggtt ccgccttcga agcttcgaca caaccggaat cacgggcatg atttgaaccg
      421 tgcgttgttt tcaaacgaca aaccggcgac ggtccgcaaa aatatagcta cgcccggcat
      481 caatcgtcgc ttacctgacg agaccgttct gataacctag cgaacaatcg aaaacgggag
      541 atttcgatgc gcagattttt gatcctgctg gccacgcttt cgtgcctgag cctcagcaat
      601 tgcggctaca acaccatcca gagcaatgac gagcagatca aggcgaactg gtccgaagtc
      661 gtcaaccagt accagcgccg ctccgatctg gtgccgaacc tcgtcaattc ggtgaaggga
      721 tttgcgcagc aggagaagga cgtgctgctc ggcgttacca acgcgcgcgc caaggtcggc
      781 agcgtccagg cgacgcccga agtcttgaac gaccccgcgg cttttcagaa attccagcag
      841 gcgcagggcg aattgagcag cgcactgtcg cggcttctgg tcgtgaccga gaattatccg
      901 cagctcaaat ccgatgcgct gtttcgcgat ctgatgtcgc aactcgaagg caccgagaac
      961 cgcatcacgg tcgcgcgcaa ccgctatatc aagtcggtgc aggactataa cgtcacgatc
     1021 cgtaccttcc cgaccaatct gacggcgatg ctgtttggct acaaggaaaa gccgaacttc
     1081 accgtcgaga acgagaagga aatatcgagt gcgccgaagg tcgacttcgg caccaagtag
     1141 tccggagtcg ttactgtgcg cgcgatgtcg cgagccggaa tcttcttcgt tgcgcttctc
     1201 ctgtgctggg tgtcgatcgc cgcggctgat gtcgcggtgc cgccgctcac cgggcggatc
     1261 gtcgacctca ccggcaccct gtcggccgac acgatcgccc gggagtctca acgcctgaag
     1321 gaccttcaga cgcgcaaggg cagccaggtc gccgtgctga tcgtgccgac cacgcagccc
     1381 gagagcatcg agcaatattc cattcgcgtc gcggacacct ggaaggtcgg gcgcaaaggg
     1441 atcgacgacg gcgcgctgct cgtcgtcgcc aagaacgacc acaagctgcg gatcgaagtc
     1501 ggctatggcc tcgaaggtgc gctcaccgac gtcaccgcgc ggcgcatcat cgacgaggtg
     1561 atcgtgccgc gcttcaagag cggcgatttc gatggcggta ttaccgcagg gatcacgcgc
     1621 ctggtcagcg tgatcgaagg tgagccgctg ccggtgccgc agcccgaggc ctcccatggc
     1681 atcgatgaag actgggtcag cttcatcttc agtccgttcg ggctgctgat gttcgccgcc
     1741 attgccgcgc tcctgcgcgg tctgctcggc cgattgctgg ggtcgggcgc aactgccggc
     1801 gtgatcggcg tactcgcctg gttcgtcggg ggctcgatcg ccgtcgcggc aattcttggt
     1861 tttgccgcct tcttcctcac gctgttcggt gacagcatgt ttgcggcgag ccagaatgcg
     1921 agccgcaccg ccggccgtgg cggctatact ggcggcggct ggtcgggcgg tggctggtcg
     1981 ggcggcggtg gcggctggag cggcggcagc ggtggcggat tttccggagg cggcggcggc
     2041 ttcggcggcg gcggcgcatc ggggagctgg tgaccatggg catcaaacgc gtcggcaagc
     2101 atctgctcga acaccgctgg cgcgcgcggc gcctgttccc gccgcccgtg ctcgcgcgga
     2161 tcgaagccgc gatcaaggcc ggcgagacga cccattccgg ccagctccga tttgtcgtcg
     2221 aaggcgcgct cgacggcgcg ccgctgctcc acgaccagcc ggcgcgcgag cgcgctctcg
     2281 atatcttctc gcatttgcgg atctgggaca ccgcacacaa caacggcgtc ctgatctatg
     2341 tgttgctcgc cgaccgcaag gtcgagatca tcgccgatcg cggcatcgac gcgtccgtcg
     2401 gtgccgaggg ttgggccagg atttgccacg acatggatat ggaatttcgg gccggccgtt
     2461 tcgaggccgg cgtgctcaag gggatttccg aggtctccca cgagctcgca agatactttc
     2521 ccgcacgggg tccgcatccc aatgagctgc cggacaagcc cgtggtgatt tgacgcggcg
     2581 gcggtttggc gcgcgtcagg ccgtttgcgg agtgcgacga atgtcggcca tcgcggccat
     2641 gtgctccgtg ttgactttcg gccccatcct caaaatactt gctgcaaaca ttcaggaaat
     2701 cgcgaaacga cgattttcaa ttggagagga acgtcatgcc gaagacaggc atcaagccgg
     2761 gtacgtcacg cagaacgctt ctgaagaatt ccgcggcagc cgctgccgtg ttgtcgctcg
     2821 attggtcgcg tgcacgagcc gaagccgaaa cggtgcgcat gggcgtgatg tacgatctga
     2881 ccggcccgtt tgcggcgggc ggatcggtcg ccttctcggt cggcacgcag atcgccatcg
     2941 acctcgtcaa cgagaagggc ggggtcgcgg gcaaatacaa ggtcgacccc gtcaacgtcg
     3001 attcccagag caagccagac gtcgccatca acgagggcga gcgcctgatc agccaggaga
     3061 aggtcgatgt catcaacggc gtttttgcca gtgcccaggc ggtgccgctc gcagccaggg
     3121 tcgagcagca caagaaaatc ctctggatca cgaccgcggt ttcgaccgcc gtgttcaagg
     3181 acaagaacct gcgctatgtc ttccgcgggc agatccactc cgatcagtac gggcaggcct
     3241 ttgccggctt cctttccgaa cacgccaagg ccaagctcgg catcgaacca aaggacgtaa
     3301 aggtcgccct cattcacgag gacggcccct acggcgtcgg cgtggccgct gcggacgagg
     3361 cctacgcaaa ggaggcaggc ctcaacgtcg tgctgaagga gggttattcg gcctccgcgc
     3421 ccgacctttc ggtgctggtg accaagatca agcgcgcccg cgtcgatgtg atctcgcatg
     3481 ctggatacaa ccccgacatc acgctctttc ttcgccaggc gcgcgagggc ggcctgcgct
     3541 tcaagatgct gttcggcgcc ggcgcaggct acagccagct cgacaaattg cgcgcgacct
     3601 tcggcccgga tatcgacaat ttctgcaata tcgatccggt gccggcgcaa ttgctcgatc
     3661 cggcaaagct cgcgcccggc gtcggcgatc tcatcaagac catggtcgag cgcttcaagg
     3721 ccaagaccgg cgcgaccgaa gtgccgccgc actgctcgat gggtttcaac cagacctggg
     3781 tgctgctcaa caacgtgctg ccggtggcca aggagaaata tggtggcttc gaccccgagg
     3841 ccattcgcaa ggccgcgctc gatgtggaca ttcccccggg cggcaccatt cagggctacg
     3901 gcgtgaaatt ctacccgccg gataccccga tgtcgggcca gaacgaacgc tcgacgccgg
     3961 tcgtcatgca aaatgccggc gagcatattt cggtggtctg gccgaccaac atccgcaccc
     4021 aggagccggt cttcccgttg ccgaaatc
//
LOCUS       DATLJT010000590        19389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3022313,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000590 DATLJT010000000
VERSION     DATLJT010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022313"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="terL"
                     /locus_tag="VM910_13685"
     CDS             <1..1119
                     /gene="terL"
                     /locus_tag="VM910_13685"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HVI63618.1"
                     /translation="FPRTRLNPKKATEGEFETTRRGYRFTTSVGGTLTGRGGDVLIID
                     DPTKGNDANSVVALEGAIDWFRNTALSRLDNPGESFIIVTMQRLHMNDLSGILIESGW
                     PKLVLPAIATEPMDYVVADREVYHRPVGQLLQPDRDSMEALEEIRREVGSRVFAAQYQ
                     QNPTPPDGNLIKAAWLKRYQVALDRTSYRHVVLSCDPAGKPGILNDYTAITIAGVVNK
                     ELHLLHVARGHWSVLQMRDQISALAEQWKPDLVLIEDTSSGMGLIQLLREQTSLSIVG
                     RHPKDDKETRMCRHQGRFEAARVLLPVEAPWLADFESELLSFPSGRYDDQVDAFMLFL
                     DWFSENEPNINMTWATPIVITRADMGLPPFIKDWSCHY"
     gene            1167..1469
                     /locus_tag="VM910_13690"
     CDS             1167..1469
                     /locus_tag="VM910_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63619.1"
                     /translation="MSLLDDFLAHRKMCRELEVFARDLAKQIKDSAADISPKHLHTGY
                     EFGPVQQWLDFDIIAAARRGDRDTVLRIAEQSRQWIDECRSDYAAFLAERKRKGLL"
     gene            complement(1797..2828)
                     /locus_tag="VM910_13695"
     CDS             complement(1797..2828)
                     /locus_tag="VM910_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63620.1"
                     /translation="MRSLAIPVFAVAALICAHGGSRADSAAVASFYAGRRITLVVGTG
                     PGAGYDAYARLVARFMKPHIPGQPTIVVQNMPGSAGVRAAGYMYNAARKDGTFIAMNP
                     ADYLLAELWNPEQMRFDSRKFGWLGTVSTITDVLAVFRSSGVRNIADARTKELTIGAT
                     AVIATNSLQPALANALLGTKFRIVKGYNGGSDPMTLAMERGEIDGRANSWASWKLLRP
                     SWIEENLLSFLLQFGPKAPDLPADVPAMADLVTTARDRAFVSMLDITQHTGRAMFTTP
                     DVPGERLEALRMAFDATMADPAFVAEMMRLAFDLRPHSGADMQAAIERVMQERDTVVP
                     QLKALLKLD"
     gene            complement(2868..3716)
                     /locus_tag="VM910_13700"
     CDS             complement(2868..3716)
                     /locus_tag="VM910_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI63621.1"
                     /translation="MNTTSRRLSGKVALITGCGPNINGGIAYGLADEGAKVVCVDVRA
                     DYAEACARAILQRGGEAVAATCDVSREDQVMATLVKARSTFGVVDILINGAVLQIRKG
                     VLDLTAEEFHRQIDVSLIGALLFTKHVARGLIRNNKPGSIISIVSTEGHQGNPGNVGY
                     GTVKAGLLNFTRSVAMELAPYGIRVNSLSPTGTDPAEGLERAAAWGVTWEAGQGRSPN
                     TDTTSGDQGVPLGKRPSPRHYAKGAVFLASDDAEMITGFDLRIDAGTVARYWRWNPGT
                     KIAPAY"
     gene            complement(3713..4555)
                     /locus_tag="VM910_13705"
     CDS             complement(3713..4555)
                     /locus_tag="VM910_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HVI63622.1"
                     /translation="MTLGALGGPHTFNARAAQRLMRHYPRFSEIVYFPTSEAVMQAAL
                     RGDVMAACGQEQTSRDGFHVGMQARIAAPGSRLYAVAEIAQAYHCSLLGKPGARLEQV
                     RRILGHTGSVAHSRGWLERNLPGAAIEIVETNSMAAARAVLDGDGAVASVGSPDLAKE
                     LDLTEMSRDIDDLTVVNYRAVSLEPLFDPTPDRLAVAGRFRGEPEMSRMVCALRDNGF
                     DLHAISPRATGGALYEYDYLFRFRGGGGLDAVLSVLSRVPSARLAGAWRSSGAPDEST
                     IGSP"
     gene            complement(4636..5682)
                     /locus_tag="VM910_13710"
     CDS             complement(4636..5682)
                     /locus_tag="VM910_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63623.1"
                     /translation="MSDVLGIGITHYPSFLPRVSAPGSLRRILQDPGLPDRYRKPEGW
                     PARMREEWGDDEGLKFAEQHREGVVGAMRETRAALDAFKPDFVVIWGDDQYENFREDV
                     MPAFCILAYDAVDIQPWLKSERPNAWGEAKDTTFRIKGHRAGAKYLTGKLIEQDFDVA
                     YAYKPLHESLSHAFTNSVLFLDWDRRGLDLPIVPFSVNCYGRRVIAAQGYIESLANPI
                     PEQNLDPPSPSPSRCFDLGAACARSLMSSPWRVALVASASWSHAFLTRKNSFLYPDTA
                     ADRALFDALKAGNYDTWRSVTLDQIEASGQHEMLNWFCLVGAMAELGRKPNRAEFFES
                     NIMNSNKVITTFLP"
     gene            complement(5740..6270)
                     /locus_tag="VM910_13715"
     CDS             complement(5740..6270)
                     /locus_tag="VM910_13715"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HVI63624.1"
                     /translation="MTAGTANADGVPASQDNLLLRLAVEYFLFEEAAHLDNWRLDEWL
                     SLFTKDARYLVPTTDLPDGDPARDMMFIDDDYERLKGRVTRLKSRHAHREYPSSRTRR
                     LITNVRLTRIDASEVEVEASFAVYRSRNEMIAPYVGIYRYTLARQDGGAFLIRRRRAE
                     LDLERLTDHGAVSIIL"
     gene            complement(6267..7604)
                     /locus_tag="VM910_13720"
     CDS             complement(6267..7604)
                     /locus_tag="VM910_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVI63625.1"
                     /translation="MDLANLIIEDAAAGLFRVHRSALTSTEIMTLEQERIFARSWLYV
                     GHDSEVPEPGDYRRRMVAGRPLIFVRGRDRIIRVLINSCLHRGALVCRTDVGNASAFQ
                     CFYHGWSYDTAGKLIGVPDAEGYSAEFNNREQELVSPPHVDSYRGMYFVNFGKSVPSL
                     RDHLGNARELIDLTMDSAEPLGGWTIIRGTAKFRIRANWKLLAENSTDNYHFRTVHRT
                     YTEYLTERRERAGAPAPSVNRINNSRSLALDNGHVAMLTQSVGRAIANPSPVWSQEAV
                     AETRRVRDELVKIYGEERGLQMATVSRFLIIFPNYAFQDTQSGFRVRQWWPTSPGEME
                     VNQWEFIPRRERADLTAYRLEGAAMFLGPGGLGTPDDIEALESCQIGFGAPEVEWSDI
                     SRGMQRLPRSDDELAARGFWRQWHALIQGLDGAARTGDVAAPDSLPPKMELRA"
     gene            7807..8799
                     /locus_tag="VM910_13725"
     CDS             7807..8799
                     /locus_tag="VM910_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HVI63626.1"
                     /translation="MLLLTHDDIAGLLAPEEIIEALREALLEQSKGLVQVPPRTTIDS
                     SSGLGWLRTMPAILNASKVMGFKAMHSTPGVGVGYFVDLYDLPTGELLAMMDADWLTA
                     QRTAATAAIAVDLLARNTIRTVGVLGSGEQARSMLIAASKVRQLQQVNVFSPTPAHRH
                     AFAEQIGREQRLNITPVERPEQAVVGCDLLLSVFRAGKEPFVAADWITPGMHICAASS
                     VRPAARELEDHIWRKAAVVAVDDRTHAFESGDGRSAIASRSFDAECAIELWELVGGQK
                     RGRSADSDVSDVTLFKSVGTALQDLAVAKAVYRRARQKGVGRDLGRFPKMRPHA"
     gene            complement(8948..9961)
                     /locus_tag="VM910_13730"
     CDS             complement(8948..9961)
                     /locus_tag="VM910_13730"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI63627.1"
                     /translation="MPMKYKKHEAKEWARQNWHGLCNVIIPSYSSDLKRLNESGIRHD
                     VRRNIELGYWGALLVSEAATTDAEYVEFMEIAVDEAKGRHYFLFHGCFDTVEDIVRLA
                     KAAEDIGADGLLLGHPTSFYPQSEKELYDFTEYVCNRTALAVVGFAQPHWNFNRLHPS
                     GYPPNVMLEAARLENLVACKFEVGGISGHYDFWLKLKGTNVLYSDPMEANFPVSVELF
                     GQQWSGTSGYEIFGGAAPKIFKLLRDGKHDEAMGLYWRVDPVRQLRVQLSAYMSGAHF
                     INRSLWKYWAWLNGYNGGPLRQPVNKVNDREMRLSREAMLRAGFDIKDESFADFFIGR
                     HPN"
     gene            complement(10151..11038)
                     /locus_tag="VM910_13735"
     CDS             complement(10151..11038)
                     /locus_tag="VM910_13735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63628.1"
                     /translation="MRIIVPFDPGAAVDVVARILARGLSVQWDRPVIVENRPGGSMIV
                     GARFVANSEPDGDTLLLCLDELFTTLPHLSGNPSFDANKELVPVAQIAKILMLLVANR
                     GVAADTLPELIDLARAKPGTLSYGSSGPGSATNLAMEMLKHLTNIDILHVPFRGLAPA
                     LTATTAGTVQMTMIGYGTARGMLEDGKQIKPIVVTSPQRVAALPDLPTTGEFGYPQVD
                     STSSLTLAAPARIRAETVGRLGTALSRELSGASEVRKQIEARDIVVTNIAGKPLAEEI
                     ARRFRLNGEAVRIAGVQDN"
     gene            complement(11208..11381)
                     /locus_tag="VM910_13740"
     CDS             complement(11208..11381)
                     /locus_tag="VM910_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63629.1"
                     /translation="MVEYRQEGAQIEQLEIDAHPTSIVTTFALVDRQVAVDNEPGRAV
                     AARRLRRAGTESA"
     gene            11797..12639
                     /locus_tag="VM910_13745"
     CDS             11797..12639
                     /locus_tag="VM910_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63630.1"
                     /translation="MKVLVTGASGRLGKVVSHHLTKHGLEVLGIDQTPNPSHKPSWIG
                     NLCQTGDLFEACAGAEAIVHLAAIQAPNLAPDSVTFNNNVSSTYNVFKVAADLKIRRV
                     VTASSNAVYGFVYARTFPVPDYLPLDEDHPCRPQDPYGLSKQAGEMIAASFALQTRAS
                     AVSLRFVGINFDPTFSIIKERMKDPRSRLSGFWSYVDARDAAEACRLALDLAPVGHHI
                     FNIAAPTSSMKEPTDALLKQLLPTVSKKQSGLKGNWSGTDSNKSTRELGFHAEHLWEK
                     YLTP"
     gene            complement(12746..14068)
                     /locus_tag="VM910_13750"
     CDS             complement(12746..14068)
                     /locus_tag="VM910_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI63631.1"
                     /translation="MATFKAPQTHQEFMTEYPRTTADSLAGQMLRHYWHPVCFSRDLK
                     DIPYGVRLLGEDLVAFRNTNGALGLVGRSCAHRAASLEYGQVTSEGLMCSYHGWTFNS
                     EGRCVAMPLEPANCPLMKKASIPSYPVQEYGGVVWGYLGANKGNPPPLPKIDMLVRDD
                     GELSMERGDIRDYTYLNWLENVADNGHVHVLHTLLPGVIPDDIRPYINTTVDPDWRNS
                     KFSVFETNYGMKTVLVQNTADPNLKFVNTLSIVFPNCWRMANNGGIPPDWSNDRRESG
                     GLLRIIDDTHFEVMRLSFVRQGNYRKGFRASDPKLDRSIKDRLRGTVEKKDHDYRQYP
                     GWEGSTSLEDLIMQQSQGSPADREELLASSDAGVVRLRRIFRKCMEQVAADEQPKAIL
                     NNDQGVIEADTFKGLVTVDELKVGPENMPSSRDGRGLLRDATGKLLFA"
     gene            14376..15656
                     /locus_tag="VM910_13755"
     CDS             14376..15656
                     /locus_tag="VM910_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI63632.1"
                     /translation="MASTDDIVARIDREALIRFALEICNIDSAVPHEAAVAEHIYQWM
                     LREGFTARRIGLLPDRFNVMGVLPGTGGGHSLMFNGHLDTYAARERDLVHLDPAAAVY
                     HSAWREDDLLIGEGIANDKGPIAAFLMAAKAIKDSGQRLKGDLVLTCVIAETSREPVG
                     CEPGIPRESKDLGARFLVTHGGVADHVVVAEGTGFGLAWVEAGKFWYKVTLRSAQPPF
                     YTPYLPARTTLARSPNMIVASAAAIAALEDWAAAYQQRNTRQTGGGTIVPKVQIGAIR
                     SGDPSRPILSPQLCELFLDVRALPGQDPLATRDEIRAVLNQVGLTADIDLYHFRPGYE
                     AKKIDRLAETVRRAHRATFGEEPKMSNPETSSMWRDTNVFAEVGIPAMSYGPRTAAHS
                     FKRALTIDSLYQAACAYTRIALDLCNQDKEPVHA"
     gene            15732..16499
                     /locus_tag="VM910_13760"
     CDS             15732..16499
                     /locus_tag="VM910_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63633.1"
                     /translation="MLKARVIITQIAVIVAILAAWESAVDAHWIAADILPPPSTVMVT
                     LGGLLRSMDFLANAGDTLVRVLAAFAIGAPLALLVGFIMGENITIGKSFSPIFNLVLA
                     VPQSIFLPIFALVFGLGFTEKLMFGITHVFFVVAVSTMAAVHQVSHAHVIAARSFGAT
                     RLRIWLSIYLPAMAPHVITGLRVGMIFNIIGILLAEMYASQSGLGMLLLRWSESYEVN
                     QLMAATIFISVVTILINEAMRLWEAGVGRWQSARMAE"
     gene            16496..17293
                     /locus_tag="VM910_13765"
     CDS             16496..17293
                     /locus_tag="VM910_13765"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63634.1"
                     /translation="MTKPTVIETRKVTKTYNVNAAPVPALVDISFAAEENSFVSLVGP
                     SGCGKSTLLQILAGLIPSSSGEVLVDGMPVRKPMPDKIAVVFQDATLLPWKTVIQNIE
                     FPLEVQKVPPRERRECARAMLSLVGLSDFAERYPHELSGGMRQRVSIARGLAQNPRII
                     LMDEPFGALDEQTRLKMGNELLHIWSRTNKTIFLITHSLTEALFLSDTVLVMSQRPGR
                     IVDVIKTALPRPRTYDMIGSREFGEARNRIWELIGGDEPTAAERVGL"
     gene            17290..18045
                     /locus_tag="VM910_13770"
     CDS             17290..18045
                     /locus_tag="VM910_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63635.1"
                     /translation="MSRPTLVRLIVIVAILVVWESVRELNLIGPLLLASPSEIGAAFA
                     KSWPQFLTAFQITLVEIVAALAIALVLGVSVGLIAGSTVFMSMVSGPLLVSLFAVPLV
                     TWYPLFMVWFGIGGASKIAYGVVSGFFPVAINTMNGIRNFDPQYVMYGRAIGCSPLQI
                     VARILFPLALPSIIAGLRIGVALVIIGVIVAEMLGSLRGIGYLITSYRNLYEIGGVYL
                     GILLSVLCALLANLGLSALERHFTRWREMQMTQ"
     gene            18336..19307
                     /locus_tag="VM910_13775"
     CDS             18336..19307
                     /locus_tag="VM910_13775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63636.1"
                     /translation="MTSRRSSVRGLLRAGAFFSLLLAGSTASLASDTVRVGVGAVYPT
                     YSVFFAGNKLGIYKKHDLDVEITTFRGGPATQEALAAGSIDLSAIAPAAVNLAVKKGV
                     REKIVAMFTPPAPDGWYIMVPTDSPVKTMADLNGKSVGVTQKGSLTDFWTHRVASLSA
                     ITVSTVPLGGGVVPGVKSKQVDAAIVWPIFSYKSMVDGSLGPVFSLGEKLPPSVSEGM
                     AAHEDMIEYRPAVLKRWLAATSETISHMQADKAWTEDFLKEYFKEDNPQVLELVYKEF
                     IMHIRANGLMKKEEMEDALQAAQFLGDNEPLSPDKIFSTAFTPIPTK"
BASE COUNT         3705 a         6072 c         5875 g         3737 t
ORIGIN      
        1 tttccccgca cccgtctcaa tccaaaaaag gctaccgagg gtgaattcga gaccacccgt
       61 cgcggatatc gcttcacgac atccgttgga ggcactttaa ccggccgcgg cggcgacgtt
      121 ctgatcatcg atgatcctac aaagggcaat gacgccaatt ctgtcgtagc acttgagggc
      181 gcgattgatt ggttccgcaa caccgccctg agccgactcg ataatccggg cgagagcttc
      241 atcatcgtga cgatgcagcg tctccacatg aacgacctct cgggaatcct gatcgagagc
      301 ggctggccga aattggtcct tcccgcgata gccacggagc ccatggacta cgtcgtggcc
      361 gatagggaag tttatcaccg cccagtcggg caattactgc agccggatcg cgacagtatg
      421 gaggccttgg aggagatcag gcgtgaggtc gggagccgag ttttcgcagc tcagtaccag
      481 cagaatccga cccctccgga cggaaatcta atcaaagcgg cttggcttaa gcgttaccaa
      541 gtcgccctcg accggacaag ctatcgccat gtcgtcctgt cttgcgatcc tgccgggaag
      601 cccggcatcc tcaacgacta cacagccatt accattgccg gtgtcgtcaa caaggagctt
      661 cacctcctgc atgtggctcg cggacattgg agcgtattgc agatgcggga tcagatttcc
      721 gcgttggccg aacaatggaa gccggatctc gtcctcattg aggatacgag cagcggtatg
      781 ggattgatcc agcttctcag ggagcaaact tcgctaagca tcgtcggtcg acatcccaag
      841 gatgataagg aaacgcgcat gtgtcgtcac caaggacggt tcgaagcagc ccgcgttctg
      901 ttgccggtag aagctccatg gttggcagac ttcgaaagcg aactcttgtc gttcccaagt
      961 ggccgttatg acgatcaagt cgacgcgttc atgctgtttc ttgattggtt ctccgagaat
     1021 gaacccaaca tcaatatgac ttgggccaca cccatcgtga tcacacgcgc cgatatggga
     1081 ctccctccgt ttatcaaaga ctggtcctgc cattactgag acgcccccgt gtcactccgc
     1141 gctggcgatt gaaaggaagg tgcagtgtgt cgttgctcga tgatttccta gcgcatcgga
     1201 agatgtgtcg ggagcttgag gttttcgcgc gtgacctcgc aaaacagatc aaggactccg
     1261 ctgccgacat ctcgccgaaa catctgcaca ccggctacga gttcggtccg gtgcagcaat
     1321 ggctcgactt cgacatcatt gcggctgcac gacgcggtga tcgcgacacg gtgctacgca
     1381 tcgccgaaca aagccggcaa tggatcgacg agtgtcgcag cgattacgca gcgttcctgg
     1441 ccgaacgaaa acgaaagggt ttgctgtgat ggctcgcaac gccggggagg ccgacacggt
     1501 acgtttccat tttcggccgt gcctacggac gcatgagccg ccgcagaggc gagatcacat
     1561 cgtcgctgag gtaaagcgta ccgactatga tgttcaagtc gcgaagcgcg atcttggcaa
     1621 gattctcgac cgcatcgagg ccgtaaacaa gccgaaggcg gcctaaccgg cggagtgcaa
     1681 tatcacaggg tgtcgatatc ttgcggaaag acgcgcgcgg cgggccgccg cgtcacagcg
     1741 ccacggcgaa cgagtagtgc ccatcttcat cgaccgtggc gcaccagtgt cacggctcag
     1801 tcgagtttga gcagtgcctt caactgcggc actacggtgt cccgctcctg catcacccgc
     1861 tcgatggcag cttgcatatc ggcgccgctg tgcgggcgaa ggtcgaacgc gagccgcatc
     1921 atctccgcca cgaaagcggg atcggccatg gtcgcatcga acgccattcg cagcgcctcg
     1981 agccgttcgc cagggacatc tggcgtggtg aacatcgcgc ggccggtatg ctgggttatg
     2041 tcgagcatgg atacaaaagc cctgtcgcgc gccgtagtga cgaggtccgc cattgccggc
     2101 acgtccgccg gcagatccgg agccttcggg ccgaactgca gcaagaagct caacagattc
     2161 tcctcgatcc agctcgggcg cagcagtttc cagctggccc agctgttcgc acgcccgtcg
     2221 atttcgccac gctccatcgc caaggtcatc ggatcgctgc caccgttgta gcctttgacg
     2281 atgcggaact tggtgccgag cagcgcattg gcgagcgcgg gctgcaacga gttggtcgcg
     2341 atcacggccg tggcgccgat cgtcagctcc ttggttctag cgtctgcaat gttccgcaca
     2401 ccgctcgacc gaaacaccgc gagtacgtct gtgatggtgg agacggtccc aagccagccg
     2461 aacttgcgcg aatcgaagcg catctgctcg ggattccaca gttcggcgag gagatagtcg
     2521 gccgggttca tggcgatgaa ggtgccgtcc ttgcgcgccg cattgtacat gtagccggcg
     2581 gccctgacgc cggccgagcc cggcatgttc tgcaccacga tggtgggctg gcccggaatg
     2641 tgcggcttca tgaagcgcgc gaccagacgc gcgtaggcgt cgtacccggc gcccggcccg
     2701 gtgccgacca cgagcgtgat ccgcctgccg gcatagaagc ttgcgaccgc cgcgctgtcg
     2761 gcgcggctgc caccgtgcgc acatatcagc gctgcaacag caaacactgg aattgcaagt
     2821 gatctcacgt aacccatcgc agggctttgt ccgagcggat gaaggctcta gtacgcaggc
     2881 gcaatcttgg tgccgggatt ccagcgccag tagcgggcga ccgtgccggc atcgatacgc
     2941 agatcgaacc cagtgatcat ttccgcatcg tccgaggcca ggaacacggc gcccttcgca
     3001 taatggcgtg gactcggacg cttgccgagc ggcacaccct gatcgcccga cgtggtatcc
     3061 gtgttgggcg agcgtccctg accggcctcc caggtcacgc cccatgccgc cgcgcgctcg
     3121 agaccttctg ctgggtcggt ccccgtcggc gacaggctgt tgacgcgaat gccatagggc
     3181 gcgagctcca tggcgacgct gcgggtgaaa ttgagcagtc cggccttcac cgtgccatag
     3241 ccgacattac ccggattgcc ctgatgcccc tcggtcgaga cgatggagat gatgctgccg
     3301 ggtttgttgt ttctaatcag gccgcgcgcg acgtgcttgg tgaacaacaa ggcgccgatc
     3361 aggctgacgt caatctggcg gtgaaattcc tccgccgtca gatcgagcac gcccttccgg
     3421 atctgcagca cggcaccgtt gatgaggata tcgaccacgc cgaatgtgct tctggccttg
     3481 accaaggtcg ccatgacctg atcctcgcgc gacacgtcgc aggtcgcagc gacagcctcg
     3541 ccgccgcgct ggagaatggc ccgcgcgcag gcctcggcgt agtcggcacg aacgtcgacg
     3601 cagacgactt tggcaccctc gtcggcaagg ccgtaggcga tgccgccgtt gatattcggg
     3661 ccgcagccgg tgatgagggc tactttgccg ctgaggcgac gggaggtcgt gttcatggcg
     3721 atccgatcgt tgactcgtct ggcgcgccgg agctgcgcca ggcaccggcg aggcgcgcgg
     3781 acgggacgcg ggacagcacg gatagcacgg cgtccagtcc gccaccgccg cggaagcgaa
     3841 acaggtaatc atattcgtag agtgcgccgc cggtggcgcg cggggagatg gcatgcaggt
     3901 cgaagccgtt atctcggagc gcgcagacca tgcggctcat ctccggctcg ccgcgaaagc
     3961 gcccagcaac tgccaaacgg tcgggcgtgg gatcaaacag cggctcgagc gacaccgccc
     4021 gatagttgac cacggtcaaa tcatcgatgt cgcgggacat ctcggtgagg tcgagctcct
     4081 tcgccagatc cgggctgccg acggaagcaa ccgccccatc gccatcgagc accgcgcgcg
     4141 cagcggccat cgagttggtt tccacgatct cgatcgccgc gccgggcagg ttgcgttcaa
     4201 gccagccgcg gctgtgcgca accgaaccag tgtgaccaag gatacgtcgc acctgctcca
     4261 gccgcgcgcc gggcttgccc agcagcgagc agtgataggc ctgtgcgatt tcggcgacgg
     4321 catagagccg cgatccgggt gcggcaatac gcgcctgcat gccgacgtga aaaccatccc
     4381 tggaggtctg ctcctggccg cacgcagcca tgacatcgcc gcgcagcgcg gcctgcatca
     4441 ccgcctcgga cgtcgggaag tagacgatct cgctgaaccg cgggtagtgg cgcatgagcc
     4501 gctgcgcggc ccgcgcattg aaggtgtgcg gcccgccaag tgcaccgagc gtcaaaccag
     4561 attttcccat cggtgcgctc tacttcgccg ccaacggcga ttgcccaacc gccgatacgc
     4621 cccccctgcc ccgagctacg gcaggaaggt ggtaatgacc ttgttcgagt tcatgatgtt
     4681 ggattcaaag aactcggcgc gattgggctt gcggccaagc tcggccatgg cgccgacgag
     4741 gcaaaaccag ttcagcatct cgtgctggcc gctggcctcg atctgatcga gggtcaccga
     4801 gcgccaggtg tcataattgc cggccttcag cgcgtcgaac agcgcgcggt cggcggccgt
     4861 gtccggatag aggaacgaat tcttgcgcgt gagaaaggcg tgcgaccagc tggcggaagc
     4921 aaccaacgcc acgcgccagg gactggacat gaggctcctg gcacaggccg cgccaagatc
     4981 gaagcaacgc gagggcgacg gcgagggcgg atcgagattc tgctcgggga tcgggtttgc
     5041 caggctttcg atgtagcctt gcgcggcaat gacccttcgc ccgtagcagt tcaccgaaaa
     5101 cggcacgatc ggaaggtcga ggccgcgccg atcccaatcg agaaacagca ccgaattggt
     5161 gaaggcgtgg ctcagcgact catgcagggg cttgtatgcg taggcgacat cgaaatcctg
     5221 ctcgatcagc ttgccagtca gatatttggc gccggcgcga tggcccttta tgcggaacgt
     5281 ggtgtccttg gcctcgcccc atgcatttgg ccgctcgctc ttcaaccagg gctgaatgtc
     5341 gaccgcgtcg taggcgagga tgcaaaatgc tggcatcacg tcttcgcgaa aattctcgta
     5401 ttggtcgtcg ccccagatca cgacgaaatc gggcttgaac gcgtcgagcg cggcgcgggt
     5461 ctcgcgcatc gcgccgacca cgccttcccg gtgctgctcg gcgaacttga gcccctcgtc
     5521 gtcgccccat tcctcgcgca tgcgcgcggg ccagccttcc ggcttacggt agcggtcagg
     5581 cagaccggga tcctggagaa tgcgccggag cgagccgggc gcactgaccc gaggcaggaa
     5641 gcttggataa tgggtgatgc cgatacccag aacgtcgctc acgatgtctg cctccaatcg
     5701 caaccgcagt tcttcaccgc gaccggcgta ccccgaccgt cacaggataa tgctcacggc
     5761 gccatgatcc gtcagccgct cgaggtcgag ctcggccctg cgccggcgta tcaggaacgc
     5821 gccgccgtcc tggcgggcga gggtatagcg atagatgccg acatagggcg cgatcatctc
     5881 gttgcgggac cgatacaccg cgaacgaggc ttccacctca acctcactcg catcaatgcg
     5941 cgtcagccgc acgttcgtga tcaaccgccg cgtccgcgac gacgggtatt cgcgatgcgc
     6001 gtgccggctc ttcaggcgcg tcactcggcc cttgagtctc tcgtaatcgt catcgatgaa
     6061 catcatgtcg cgggccgggt cgccgtccgg caaatcggtg gtcgggacta gatagcgcgc
     6121 atccttggtg aacaggctca gccattcgtc gagccgccag ttgtcgaggt gcgccgcctc
     6181 ctcgaacaga aaatattcga ccgcgaggcg caacagcaga ttgtcctggc tggcgggcac
     6241 gccatctgca ttcgctgtgc cggcggtcac gcacgcagct ccatctttgg gggtaacgaa
     6301 tccggcgccg ccacgtcgcc ggtccgcgcg gcgccgtcca gaccttgaat gagcgcgtgc
     6361 cattggcgcc agaatccgcg tgccgccagc tcgtcgtcgg agcgtggcag ccgctgcatc
     6421 ccgcgcgaaa tgtctgacca ttcgacttcc ggcgcgccaa atccgatctg acaagattcc
     6481 agcgcttcga tgtcatcggg cgtgccgagg ccccccggcc ccagaaacat ggcggcgcct
     6541 tccaggcgat acgcggtcag gtctgcacgt tcgcggcggg ggatgaactc ccactggttg
     6601 acttccatct cgcctggcga cgtcggccac cattggcgca cgcgaaagcc ggattgggtg
     6661 tcctggaacg cgtagttggg aaagatgatc aggaagcgcg agacggtggc catctgcagc
     6721 ccgcgctctt caccgtagat cttcaccagc tcgtcccgca cgcgtctggt ttccgcgacc
     6781 gcctcctggc tccagaccgg gctcgggttg gcgatggcgc gtccgaccga ttgcgtcagc
     6841 atcgccacgt gcccattgtc cagggcaagg ctgcgtgaat tattgatgcg gttcacgctg
     6901 ggcgccggcg ccccggcacg ctcgcgccgc tccgtcaggt attcggtata ggtccggtgc
     6961 acggtgcgga agtgatagtt gtcggtgctg ttctctgcca acagcttcca gttggcgcgg
     7021 atcctgaact ttgctgtgcc cctgatgatc gtccagccgc ctagcggctc ggccgaatcc
     7081 atggtcagat cgatcagctc gcgcgcattt cccagatggt cgcgtagcga cggcaccgat
     7141 tttccgaaat tgacaaaata catgccgcga tagctgtcga catgcggcgg ggataccagt
     7201 tcctgctccc ggttgttgaa ctcggccgaa tagccctcgg catcgggaac gccgatcagc
     7261 ttgccggccg tgtcgtacga ccagccgtga tagaagcact gaaaggcgct ggcattgccg
     7321 acgtcggtac ggcagaccag cgcgccgcgg tgcaggcagc tgttgatgag cacgcgaata
     7381 atccggtcgc gcccccgcac gaagatcagc ggtcgcccgg caaccatgcg gcgccggtag
     7441 tcgcccggct cgggtacttc cgaatcgtgt ccgacataga gccaactgcg ggcaaagatc
     7501 ctttcctgct ccaaagtcat gatctcggtt gaggtcagcg cggaccggtg cacgcggaac
     7561 aggccggcgg ctgcatcctc gatgatcaag tttgccagat ccacggaacc ctctcagcag
     7621 tgaagctgca tgcccgtagc tcgggcgcgc gccggccaag ctaatgcgcc ggcaagtccg
     7681 cggcaaatgt aaagattggc gtgattgata gcgagaacgg atcgacccag ctcttgcatt
     7741 tgccggccat aagagccttc atctgcgcca tcggcatcgc ccgacggacc acagggggcc
     7801 ttcaccatgc tactgctcac gcatgacgac atcgccggcc tgcttgcccc ggaggagatc
     7861 atcgaggctt tgcgggaagc gctgctcgag cagagcaagg gcctcgtgca ggttccgccg
     7921 cgcaccacga tagactcatc gtccggactg ggttggctgc gcacgatgcc ggcaatcctc
     7981 aacgcctcca aggtgatggg cttcaaggcc atgcattcca cgccgggcgt tggggtgggt
     8041 tatttcgtcg acttgtacga tctgccgacc ggcgagcttt tggcgatgat ggatgccgac
     8101 tggctgaccg cgcagcgaac ggcagccacc gccgcgatcg ccgtcgattt gctggcacgc
     8161 aacacgatcc gaaccgtcgg cgtcctcggg tccggggagc aggcgcgctc aatgctcatt
     8221 gcggcaagca aggttcgtca attgcagcag gtcaacgttt tcagcccgac ccccgcgcat
     8281 cggcacgcgt ttgccgagca gatcgggcgc gagcagcggc tgaatatcac accggtcgaa
     8341 aggccggagc aagcggttgt gggctgcgat cttctgctca gcgtattccg cgccggcaag
     8401 gagccgttcg ttgccgctga ctggatcacg ccgggcatgc atatctgcgc cgccagctcc
     8461 gtgcgaccgg ccgcgcggga gcttgaagac catatctggc gcaaggctgc cgttgtcgcg
     8521 gtcgacgatc gcacgcacgc attcgagtcg ggcgacggca ggagcgccat cgcctcgcga
     8581 tcgttcgacg cggagtgcgc cattgagttg tgggagctcg tcggtggaca aaaacgcggc
     8641 cgcagcgccg acagcgacgt cagcgacgtc acgctgttca agtctgtcgg caccgcgcta
     8701 caggatctcg ccgtcgccaa ggcggtctac cgacgggcca ggcaaaaggg agtgggacgc
     8761 gacctcggcc gttttccgaa aatgcggccg catgcgtgag actgggccaa ccggacgtcc
     8821 tatcccccac aacggcagcg tgtttactgg ttcccggccc tgcgcgagcc ccgtaagagc
     8881 ggggttcgct tggccgggat gatcgccaaa tgcgacagac ctgcccaccg cgcgaaggac
     8941 gcaaagctca gttcggatgc cgcccgatga agaagtcggc gaagctctca tccttgatgt
     9001 cgaagccggc gcgcagcatc gcctcgcgcg acagccgcat ctcgcgatca ttgaccttgt
     9061 ttaccggctg gcgcagcggt ccgccgttat agccgttgag ccaggcccaa tacttccaga
     9121 gcgagcggtt gatgaagtgc gccccgctca tataggccga cagctggacg cgcagctggc
     9181 gcaccggatc gacccgccaa tagagcccca tcgcttcgtc atgcttgccg tcgcgcagga
     9241 gcttgaaaat cttcggcgcc gccccgccga aaatctcgta gccgctggtg ccggaccact
     9301 gctgaccgaa cagctccacc gaaaccggaa aattcgcctc catcggatcc gaatagagca
     9361 cattggtgcc cttcagcttc agccaaaaat catagtgtcc agagatgccg ccgacctcga
     9421 atttgcaggc caccagattt tcgagcctgg ccgcttcgag catgacgttc ggcgggtaac
     9481 cgctcgggtg cagccgattg aagttccagt gcggctgcgc gaagccaacg acggcgagcg
     9541 cggtgcgatt gcagacatac tccgtgaagt cgtagagttc cttctcgctc tgcgggtaga
     9601 acgaggtcgg atgtcccagc agcaagccgt ccgcgccgat gtcctcggcg gctttggcca
     9661 ggcgcacgat gtcctcgacc gtgtcgaagc agccgtgaaa caggaagtag tgtctgccct
     9721 tcgcctcatc gacggcgatt tccatgaatt cgacgtattc ggcatcggtg gtcgcggctt
     9781 cggacaccaa gagcgcgccc cagtagccga gctcgatgtt ccggcgcaca tcgtggcgaa
     9841 tacctgattc gttgaggcgc ttcaggtccg agctgtagct cgggatgatg acgttgcaga
     9901 ggccgtgcca gttctggcgc gcccactcct tggcctcgtg cttcttgtat ttcattggca
     9961 tcggcggttc tcccaaacgg acgtggctgt gatggatgat ggtgcggcag gggcgctcgg
    10021 caatggatcc tgcgtcacta taatgcgtcc catgccacta taaggcccgc ccgacggccg
    10081 gcaaatgcaa atcgtctggc gattaatgcc tggttgtgca tcaacggcag cgcgtcatgc
    10141 aggtcagcag tcagttgtcc tgcacgccag ctatacgcac cgcctcgccg ttcaggcgaa
    10201 agcggcgcgc gatctcctct gccaggggct tgccggcgat gttggtgact acaatgtcgc
    10261 gcgcttcgat ctgttttcgg acctcgctcg cgccgctcag ctcacgcgag agtgccgtgc
    10321 caaggcggcc cactgtctcg gcgcgaatcc tggccggcgc ggccagcgtc agcgatgagg
    10381 tggagtcgac ctgcggatag ccgaactcgc cggtcgtcgg gaggtcgggc agcgccgcga
    10441 cccgctgcgg cgacgtcacg acgatcggct tgatctgctt gccgtcctcc agcatgccgc
    10501 gcgccgtgcc atagccgatc atcgtcatct gcacggtccc ggcggtcgtc gccgtgagcg
    10561 ccggcgccaa cccgcgaaac ggtacgtgca ggatgtcgat gttggttaga tgcttcaaca
    10621 tctccattgc cagattggtt gcgctgcctg gccccgacga cccgtaactg agcgtgcccg
    10681 gcttggccct ggcaaggtcg atgagttctg gcagcgtatc ggccgccacg ccccggttcg
    10741 ccacaaggag catcaaaatc ttcgctatct gggcgaccgg caccagctcc ttgttggcgt
    10801 caaacgacgg attgccggac aggtgcggta aggtcgtgaa caactcgtcc aggcacagca
    10861 gcaatgtatc gccgtccggc tcgctgttgg cgacaaaccg cgcgccgacg atcatggagc
    10921 cgcccggccg attctcgacg atgacgggcc ggtcccactg caccgacagc ccgcgcgcca
    10981 gaatgcgggc cacgacgtcg accgcggcgc ccggatcgaa cggcacgatg atgcgcactg
    11041 gcgagccgct ttgagcgagt gcggacgttt ggtcgctccc gaccgtcgtg gcgagaataa
    11101 ccgtccagaa aagcaagagc agtcgtagtc ttttcatttt ggcagcggct tctacaagca
    11161 agctctcttg cggtgttctg aagttggcgt gctgatgcaa atgataatta agcagattca
    11221 gtgccagcac ggcgcagccg gcgcgctgca actgccctgc ccggctcatt atcgacagcg
    11281 acctgccggt cgacaagtgc aaaggttgtc actattgatg tgggatgagc atcaatttcg
    11341 agttgctcga tctgcgcgcc ttcctggcgg tattcgacca cgcgagcttc aacaaagccg
    11401 ccgagctgtt gaaccggtca cagcccgcgc tctcactccg cattgggggg cctgacgggc
    11461 gggtcggcgt cccgtcgctc gagcactcga cccgtcatgt ggtgccgagc cgcagagttg
    11521 agctcaaggc ccgctgctcg acgagttcga cacgtcgctg ttgtcgatca gcggcaccag
    11581 cgagcggcag agcggccaaa tcacgatcgc atcgatcccg accgcagtaa tctatttctg
    11641 ccccgggtga tcaagcgttt caaccagcgc cacccgcaca tggcacgcga tccattctca
    11701 ataatggata catctgatga agacgggatg cgaacagcga tgtcacggat tcttcggttt
    11761 ggagtagcga cgaccgtcgt ttgaggagga caggtaatga aggttctcgt gacgggggca
    11821 agcggcagat tgggcaaggt tgtgagccat cacctgacga aacatggcct tgaggtgctg
    11881 ggcattgacc agacgccaaa cccatcgcac aagccgtcat ggatcggcaa cctctgtcag
    11941 accggagatc tgttcgaggc atgtgccggc gctgaagcga tcgtccatct tgctgcgatc
    12001 caagcgccga atttggctcc ggacagcgtg accttcaata acaacgtcag tagtacctac
    12061 aacgttttca aagtcgccgc agacttaaag atacgaaggg tggtgaccgc atcgagcaac
    12121 gccgtttacg gctttgttta tgcgcgtacg ttccctgtgc ctgactatct accgctcgat
    12181 gaagatcatc cctgcaggcc ccaggaccct tatggacttt caaaacaagc gggcgagatg
    12241 attgccgcat cctttgcgct ccaaaccaga gcctccgccg tcagtcttcg gttcgtgggc
    12301 atcaatttcg atccaacgtt ctcgatcatc aaggaaagaa tgaaggatcc gaggagtcgg
    12361 ctgtcaggct tttggagcta tgtcgatgct cgggacgcag ccgaagcctg ccggctggcg
    12421 cttgatctgg caccggttgg tcatcacatt ttcaatatcg cggccccgac cagcagtatg
    12481 aaagaaccta ccgacgcatt gctaaagcag cttttgccga cggtcagcaa aaagcagtcg
    12541 ggtctcaagg ggaattggag cggaacggac agcaacaaaa gtacgcgtga actgggcttt
    12601 cacgcggagc atttgtggga gaaatacctc acgccttgac caaagacggg atcgatctga
    12661 ggtgctagga aggtgaaggt cctcgtcgcc tcaacaacgg gttagggcct catccgttcc
    12721 gtggctgaat accacgagcc tgatttcacg caaagagaag ctttccggtc gcatcgcgaa
    12781 gcaagcctcg accgtcgcgc gatgagggca tgttttccgg accgaccttc aattcgtcga
    12841 ctgtgacaag ccctttgaac gtgtcagcct cgatgacacc ttggtcatta ttcaagatcg
    12901 ccttaggttg ctcatcggcc gcgacctgct ccatgcattt tcggaaaata cgccgaagac
    12961 gaacgacacc tgcatctgat gaggcgagca attcctcccg gtccgccggc gagccctggg
    13021 attgctgcat tatcaggtct tcaagtgacg tcgaaccttc ccagcctggg tactgacgat
    13081 aatcatggtc ctttttctcg acggtgccgc gaagcctgtc tttgatgctc cggtccaatt
    13141 taggatcact ggccctaaat cccttacgat agttgccctg acgaacgaag gacaatctca
    13201 taacttcaaa gtgcgtatcg tcaatgatgc gcaatagccc cccagattct ctgcgatcgt
    13261 tactccaatc aggtggtatg ccgccattgt tcgccatccg ccaacagttt ggaaaaacga
    13321 tgctcaacgt gttgacgaat ttcaggttgg ggtcggctgt attttggaca aggacggtct
    13381 tcattccgta gttagtttca aagacgctga acttactgtt tcgccagtct ggatcgaccg
    13441 tagtattgat atagggacgg atatcgtcgg gaattacacc cggaagaagc gtatgcagca
    13501 catgaacgtg tccattatcg gcgacgttct ccagccagtt tagataggtg tagtcgcgga
    13561 tatcaccgcg ctccatgctt agttcgccat cgtctcggac tagcatatcg atcttgggaa
    13621 ggggcggagg attgcctttg ttcgcaccca gatagcccca gactaccccg ccgtattcct
    13681 gcaccggata ggacggtatc gatgctttct tcatgagtgg acaatttgca ggttctagtg
    13741 gcatcgccac gcagcggcct tcggagttga aggtccaccc gtggtaggaa cacatgagac
    13801 cctccgaggt gacctgccca tattcgagcg acgccgcccg atgagcacag ctacggccca
    13861 ctaatcccag tgcgccgttg gtattgcgaa aagcgacaag atcttccccc aacaagcgca
    13921 ccccatacgg gatgtccttc aggtcccgcg aaaagcagac cgggtgccag taatgccgca
    13981 gcatctggcc ggctaacgag tcggccgtcg tgcgtggata ctcagtcata aattcttggt
    14041 gcgtctgggg ggccttaaat gttgccatgg tccctgcctt ccgttcgatc gataccgcgc
    14101 agagccaaga acgtgcccag tcagcatcct gcgctgcaca tgcacgttgc tgatcgcgat
    14161 cgtgtcgacg gcctaagata gcactttcga tcgacctagc gaacaagctc ccgcgaaatt
    14221 cgcttgcgcc tcctcgtcca gtcgccccac aatcgcaaac agtccttgga ggaatcggct
    14281 tggcccctgg cgcttcgatt tgcgctttca tgccgcggta agcggcacgg tattcgatct
    14341 cttgagttaa cgcgcgacct actgcgagcg caacaatggc ctcaacggac gatatcgtag
    14401 cccgcatcga ccgtgaggcc ctgatccgct tcgcactgga gatctgtaac atcgacagcg
    14461 cggtcccgca cgaggccgcc gtcgccgaac acatctacca atggatgctg cgcgagggct
    14521 ttaccgcgcg ccgcatcggg cttttgcccg accggttcaa tgtgatgggc gtgctgccgg
    14581 gcaccggcgg cggacacagc ctaatgttca acggccatct cgacacctat gccgcgcgcg
    14641 agcgcgacct cgtccatctc gatccggccg ccgcggtcta ccacagtgcc tggcgggagg
    14701 atgatctgct gatcggcgaa ggcatcgcca acgacaaggg gccaatcgcg gcattcctga
    14761 tggccgccaa ggcaatcaag gattccggcc agcggctcaa aggtgacctc gtcctcacct
    14821 gcgttatcgc ggagacgagt cgggagccag ttggatgcga gccgggcata ccgcgcgaga
    14881 gcaaggatct tggcgcgcgg ttcctggtca cgcatggcgg cgttgccgat cacgttgtgg
    14941 tcgccgaagg cacgggcttc ggccttgcgt gggtcgaggc cggcaagttc tggtacaagg
    15001 tcacccttcg ctccgcgcag ccgccgttct acacccccta tctgccagcg cgcacgacgc
    15061 tcgcgcgaag cccgaacatg atcgtcgcat cggccgcggc catcgcggcg ctggaagact
    15121 gggcagccgc ctatcagcaa cggaacacgc ggcagaccgg cggcggcacc atcgtgccca
    15181 aggtgcagat cggcgcgatc cgcagcggcg atccgtcgcg gccgattctt tcaccgcagt
    15241 tgtgtgagct cttcctcgac gtgcgcgcct tgccggggca agatccgctc gcgactcgcg
    15301 acgagatccg tgccgttctc aaccaagtgg gcctcaccgc cgacatcgat ctctaccatt
    15361 tccgcccggg ctatgaggcg aagaaaatcg atcggcttgc cgagacagtg cgccgcgcgc
    15421 accgggcgac cttcggggaa gaaccgaaga tgtccaatcc cgagacctca agcatgtggc
    15481 gcgacaccaa tgtcttcgcc gaggttggca ttcccgccat gagctatggt ccgcgcacgg
    15541 ccgcccactc gttcaagcgc gccttgacca ttgatagcct ctaccaggcc gcctgcgctt
    15601 atacgcgcat cgcgctcgac ctctgcaatc aggacaagga gcctgtacac gcttgaccca
    15661 gaccgcgtcg gcttgccaaa gcaagaggcc gatgggtgtc taatggcatg tacttcgtgg
    15721 ctagccagag tatgttgaaa gcgcgagtca ttatcacgca aatcgcagtc atcgtggcga
    15781 ttctggcggc ctgggaaagc gcggttgatg cgcactggat tgctgcggac atcctgcccc
    15841 cgccctcgac cgtgatggtt acgctcggcg gcctcctgcg cagcatggac ttcctggcaa
    15901 atgccggcga caccctcgtc cgggttctcg ccgcctttgc catcggtgcg cctctcgcgc
    15961 tgctggttgg cttcatcatg ggcgagaaca tcaccatcgg aaaatcgttc tcgccgattt
    16021 tcaatttggt gctcgcggtt ccgcagtcga tcttcttgcc tattttcgcg ctggtcttcg
    16081 gcctcggctt caccgagaag ctgatgttcg gcatcaccca cgttttcttc gtagtggcgg
    16141 tgagcacaat ggcggcggtc catcaggtct cgcatgccca cgtcatcgcc gcgcgatcct
    16201 tcggcgcgac tcggctgcgg atttggctca gcatctacct gccggcgatg gcgccgcacg
    16261 tcatcaccgg gctgcgcgtc ggcatgatct tcaacatcat cggcatcctg ctcgccgaga
    16321 tgtacgcgtc gcagagcggc ctcggcatgc tgctactgcg atggagtgag tcatacgagg
    16381 tcaaccagtt gatggcggcg accatcttca tctcggttgt aacgattcta atcaacgagg
    16441 cgatgcgctt gtgggaagcc ggtgtcggcc ggtggcaatc cgcgaggatg gctgaatgac
    16501 caaaccgacc gtcatcgaaa cgcgcaaggt caccaagacc tataacgtca atgccgcgcc
    16561 ggtccccgct ctcgtggaca tctcctttgc cgcagaggag aacagcttcg tgtcgttggt
    16621 cgggccgagc ggctgcggga agtcgacctt gctgcaaatc cttgccggct tgatcccgag
    16681 ctcgtccggc gaagtgctgg tcgacggcat gccggtgcgc aaaccgatgc ccgacaagat
    16741 cgccgtcgtg ttccaggacg cgacgctgtt gccttggaag accgtgatcc agaacatcga
    16801 gtttccactc gaggtgcaga aggtgccgcc gcgggagcgt cgcgagtgcg ccagagccat
    16861 gctctcgctg gtcggcctgt ccgatttcgc cgagcgctat ccccacgagc tgtccggcgg
    16921 catgcgccag cgcgtgtcga tcgcgcgcgg cctcgcgcag aacccgcgca tcatcctgat
    16981 ggacgagccg ttcggcgcgc tggacgaaca gacccggctc aagatgggca acgagctgct
    17041 gcacatctgg tcgcggacaa acaagacgat cttcttgatc acgcacagcc tgaccgaagc
    17101 actgttcctg tcagacacgg tcctggtgat gagtcagcgg ccgggccgca tcgtcgacgt
    17161 gatcaagacc gccctgccgc gcccgcgcac ctatgacatg atcggcagcc gggaatttgg
    17221 cgaggcccgc aaccggatct gggagctgat cggcggcgac gagcccacgg cggccgaaag
    17281 ggtcggacta tgagtcgccc cacgctcgtc cgcctcatcg ttatcgttgc gatcctggtc
    17341 gtctgggaat cggtgcgcga gctcaacctc atcggcccgc tgctgctcgc ctccccgagc
    17401 gagatcggcg cggccttcgc caagtcatgg ccgcagttcc tcacggcctt ccagatcacg
    17461 ctggtcgaga tcgtcgccgc cctcgctatc gccttagtgc tcggcgtcag cgtcggtctg
    17521 atcgccggca gcaccgtctt catgagcatg gtgagcgggc cgctcctggt atccttgttc
    17581 gcggtgccgc tggtcacatg gtatccgctg ttcatggtgt ggttcggcat cggcggcgcc
    17641 tcaaagatcg cctatggcgt ggtctccggc ttcttcccgg tcgccatcaa cacgatgaat
    17701 ggcatccgca acttcgaccc acagtacgtg atgtacgggc gcgccatcgg ctgctcgccc
    17761 ctgcagatcg ttgcccgcat cctgtttccg ctcgctctgc cctcgatcat cgccggtcta
    17821 cgcatcggcg tcgcgctggt catcatcggc gtcatcgtcg ccgagatgct cggctcgctg
    17881 cgaggcattg gctatctgat caccagttat cgtaatctct acgagattgg cggcgtctac
    17941 ctcggcatcc tgctttcggt tctatgcgcg ctcctcgcca accttggctt aagcgcgctg
    18001 gagcggcatt tcacgcgatg gcgtgagatg cagatgactc agtgacgatg cggcgcaccg
    18061 acccccgcaa tccgttgggc atcaagggcg ccgggcagcc gccgtcagcg gcgccgccat
    18121 tcccgctgcc atcgagccgg cgccagcggc ggcatcgtca cacaggttcc ggatacgccc
    18181 gaccggatca ggtcgctgct ggcgcgcggc tggtagcatg tttgagacgc cggcccgcgc
    18241 ctagtatgcg gattgctagt taatacacag gatgtagcat atcgatctgc ctcggtcggg
    18301 atagcggccc taagtcaaag agagggatgc cagtgatgac cagccgccgt tcaagcgtcc
    18361 gtgggcttct gcgcgccggc gcattctttt ctctgctgct tgcaggttcg acggcctcgc
    18421 tcgcctccga tacagtccgg gtcggggtcg gcgccgtcta tcccacctat tccgttttct
    18481 tcgctggcaa caagctcggc atctacaaga aacacgacct cgacgtcgag atcacgacat
    18541 tccgcggtgg cccggcgaca caagaagcgt tggcggcggg atcaatcgac ctcagcgcga
    18601 tcgcgccggc cgccgtgaac ctcgcagtta aaaaaggtgt gcgcgagaag atagtcgcca
    18661 tgttcactcc gcccgcgccg gatggttggt acatcatggt gccgacggac tccccggtca
    18721 aaacgatggc cgatctcaat gggaagagcg tcggtgtcac acagaaaggt tcactgaccg
    18781 acttctggac ccaccgcgtc gccagtcttt ccgcgatcac cgtcagcacc gtcccgctcg
    18841 gcggcggcgt tgttcccggc gtcaagtcca agcaggtgga cgccgcgatt gtctggccga
    18901 tcttcagcta caaatcgatg gtcgacggta gcttgggccc agtcttctcg ctgggcgaaa
    18961 aactgccgcc gtcggtctcg gaaggcatgg cagcgcacga ggacatgatc gagtaccgtc
    19021 ctgcggtgct aaagcgctgg cttgcagcca cttccgagac aatttcccat atgcaggccg
    19081 acaaggcgtg gaccgaagac ttcctgaaag agtacttcaa ggaggacaac ccgcaggtgc
    19141 tcgaacttgt ctataaagaa ttcatcatgc acatccgcgc gaatggcctg atgaagaaag
    19201 aggagatgga ggatgcgctg caggcagctc agttcctcgg cgataacgaa ccgctgagtc
    19261 ccgataagat tttcagtacg gctttcacgc cgatcccaac gaaataagga tggctcccga
    19321 cttcttgccg aacctgccga atgaatacgt gtcgcttccg aaaactcagt ccatttgact
    19381 cgcgatcag
//
LOCUS       DATLJT010000591         4440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3711717,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000591 DATLJT010000000
VERSION     DATLJT010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711717"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VM910_13780"
     CDS             <1..291
                     /locus_tag="VM910_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63637.1"
                     /translation="WLARAADLPGPPSSGWVQLWGVMLLIMATLYLPGCIEPVQVRWP
                     NVVGIVARFVLAVAYVRLGQGLRWFALYELVFAVALAWSYRQLLRAELMSRP"
     gene            516..1925
                     /locus_tag="VM910_13785"
     CDS             516..1925
                     /locus_tag="VM910_13785"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HVI63638.1"
                     /translation="MALSMSHRPPPPTKPLDTSLRGYLATNADIVTRVTKPVHMADVG
                     ALSAQSDQPIVFENIVEKPGFRLCDILVKTRRSQARALGTSPENYLRTLAYRLRQPPR
                     GLKIVKTGPVKEVVKLHNEADWTELPIPFHKDKDAAPYITAMNIIRDPETGFYNSCHA
                     GTHAVGPRRGLISFVTPHSHVIMRKYRDMGVDAMPIAFVFGVPPAYEIMANFSGLHMD
                     AWGEMEMVGTIMDRDIEMVPCETLELNVPAEAEIVVEGHVNLKDRFKVGEVTSPSMYH
                     LPHYEDLPEVEVSAITMRGDRPIYRNHQTCPDTDHQPLPRLCHEAVLYNRLSEIGVDV
                     KDVRFPTWGAALSCIIQFSYPREGMVNDALMLAMGAPWLNTKLVVAVSPDTNLDDPGD
                     VYHAIATRCDPGRDVIVVPNTRGSPYDPSARPLEGQYPWRIVGKMGIDATVKARHDPA
                     DFERAWPRNWGKVKLSDYL"
     gene            1939..2571
                     /locus_tag="VM910_13790"
     CDS             1939..2571
                     /locus_tag="VM910_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63639.1"
                     /translation="MDAIAPPKPKVLFEADPFIPYVREEDFDARLKPVLEPYKKRMGF
                     LPNALKLYAYRPEIAETLWKLNSNIMRDPSSTLDQLLKRKLAAVACATNGCAYCTAHS
                     CAMLKKSKDLGFEGWGLSEEELQDIITGTYEPKNEMERACFDYVRSASEDPTSVPDEI
                     LRRLKQHLTPQQIVELACVVGFWKFYNTVHDSLHIPVESTLLGDTGYVDL"
     gene            2558..3163
                     /locus_tag="VM910_13795"
     CDS             2558..3163
                     /locus_tag="VM910_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI63640.1"
                     /translation="MSISKLPRARAQEKRDNPAAAYILPPTVSSGALLDKGSDRRFRT
                     LVNDLFTIASRMEIVRTHLGRRMGLSGPQYSVLVAVAHLQGERGVRVGTVAQAMHVSS
                     AFIASETGKMARHGLLLKRPNPQDRRGVLLTLAPAGRLKIDRLSAEIRAINDMFFGAL
                     DGASFAALCAAARALVKGSGKAVHYVTALESEPRSALSAAE"
     gene            3331..3453
                     /locus_tag="VM910_13800"
     CDS             3331..3453
                     /locus_tag="VM910_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63641.1"
                     /translation="MAADLDACACWCGAARGDFYRVKTFSGEVTHEAMIGKVVL"
     gene            3515..4375
                     /locus_tag="VM910_13805"
     CDS             3515..4375
                     /locus_tag="VM910_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI63642.1"
                     /translation="MVNGLMLGAIYAMVAVALTLSVGVLRFLNFSIPGLFMIGGMATW
                     VLVRNGMPWPLAAGAALIAGAMASLVVERFTWRWMRTAQHFVPLVSSMAFLLLFEHLA
                     VIYWGSELRTLPALFGNADWRIAGLVISLPQLAGLLCSIALIWALTLVLARTRLGRGL
                     RTIAEDSDTALLLGVDINRIVPVVFLITGLFAAFAGLIFALNYRQVQPFMGEVVGLKG
                     ISAMIVGGMGNVWGSIAGGLIIGLVEVMSIGYFGADFVDIAVYELLLLILFIRPTGLF
                     AGAIAGQARA"
BASE COUNT          781 a         1357 c         1385 g          917 t
ORIGIN      
        1 tggctcgcgc gcgcagccga cctccccggc ccgccgtcgt ccggctgggt gcaactgtgg
       61 ggggtcatgt tgctgatcat ggcgaccctc tatctgccgg gttgcatcga accggtccag
      121 gtgcgttggc cgaatgtcgt cggtatcgtc gcgcgcttcg tgctggccgt tgcctatgtc
      181 cgtctcggcc aaggcttgcg gtggttcgcg ctctatgaat tggtgttcgc cgtggcgctg
      241 gcttggagct atcgccaatt gctgcgcgcc gaactgatgt ctcgcccgta gcagaattag
      301 ttcggcaagg cctcggcgca acacggcggg cacgttagcg gctacgagcg agcggcgcgc
      361 gcttcgctgt aaccatcggt tgcgccgcag gtctttttgc tcaggcgctg ctacgcgatt
      421 tggttggcgc gcgtcgcggc gtcgccaacg cgcttgtccg tccttgactc ccttaagtta
      481 attagtaaag ctacttaaag aaatggagga tcgcgatggc gctctccatg agtcaccgac
      541 cgccgccgcc gaccaagccg ctcgatacgt cgcttcgcgg ctatctcgcc accaacgccg
      601 acatcgtcac tcgcgtcacc aagccggtcc atatggccga cgtcggggcg ctatcggcgc
      661 agagcgacca gccgatcgtg ttcgagaaca tcgtggagaa gccaggcttc cggctgtgcg
      721 acattctggt gaagacccgc cgctcgcagg cgcgcgcgct cgggacctcg ccggaaaact
      781 atctgcgcac gctcgcctac cggctgcgcc agccgccgcg cggactgaag atcgtcaaga
      841 ccggtccggt gaaagaggtg gtcaagctac acaatgaggc cgattggacc gagctgccca
      901 ttcccttcca caaggacaag gacgcagcgc cgtacatcac cgcgatgaac atcatccgtg
      961 atcccgagac cggcttttac aactcctgtc atgccggcac gcacgcggtg ggaccgcgtc
     1021 gcgggctgat cagtttcgtg acgccgcatt cgcacgtcat catgcgcaaa taccgcgaca
     1081 tgggcgtgga cgcgatgccg atcgcgttcg tattcggcgt gccgccggca tacgaaatca
     1141 tggcgaactt ctccggtctc cacatggatg catggggcga gatggagatg gtcggcacca
     1201 tcatggaccg tgatatcgag atggtgccgt gcgagacgct ggaattgaat gtgccggccg
     1261 aagccgagat cgtggtcgag ggccacgtca acctgaagga caggttcaag gtcggggagg
     1321 tcacctcgcc ctcgatgtat cacctgccgc attacgagga cctgcccgag gtcgaggtca
     1381 gcgcgatcac aatgcgcggc gatcgcccga tctatcgcaa ccaccagact tgccctgaca
     1441 ccgaccatca gcccttgccc cggctctgtc atgaagccgt gctctacaat cggctcagcg
     1501 agatcggcgt tgacgtcaag gacgtgcgct tcccgacttg gggcgcagcg ctctcctgca
     1561 tcatccagtt cagctatcca cgcgagggga tggtgaacga tgcgctaatg ctggccatgg
     1621 gcgcgccctg gctcaatacc aagctcgtcg tcgcggtatc gcccgacacc aatctcgacg
     1681 accccgggga tgtttaccac gccatcgcga cgcgttgtga tcctgggcgc gacgttatcg
     1741 tagtgccgaa cacgcgcggc tccccctatg acccgtcggc gcgcccgctc gaagggcaat
     1801 atccctggcg aatcgtcggc aagatgggga tcgatgctac ggtcaaagcg cgccacgatc
     1861 ccgccgattt cgaacgcgcc tggccccgca actgggggaa agtgaaacta tccgactacc
     1921 tctgaaggag agagcgtcat ggatgccatt gctccgccca agccaaaagt tctgttcgaa
     1981 gccgatccct tcattcccta cgtgcgggag gaggatttcg acgcccggct caagcccgtg
     2041 ctggagccct acaagaaacg catgggcttc ctcccgaacg cgctcaagct ctatgcctat
     2101 cggccggaga tcgcggagac gctgtggaag ctcaacagca atatcatgcg cgacccaagc
     2161 tcgacgctcg atcaattgtt gaagcgcaag cttgcggcag tcgcctgcgc gaccaacggc
     2221 tgcgcctatt gcaccgccca tagctgcgcg atgttgaaaa agtccaagga cctggggttc
     2281 gagggttggg gactgagcga ggaggagctg caggacatca tcaccggcac ctacgagccc
     2341 aagaacgaga tggaacgcgc ctgcttcgac tatgttcgct cggcttccga ggatccgacc
     2401 tcggttccgg acgagatttt gcggcggctc aagcagcatc taacgccaca gcaaatcgtc
     2461 gagctcgcct gcgtggtcgg gttttggaaa ttctacaaca ccgtgcacga cagcctgcac
     2521 attccggtcg agtcgaccct cctcggtgac accggatatg tcgatctcta agcttccgcg
     2581 cgcccgcgcc caggaaaaac gggacaatcc tgcggcggcg tatatcttgc cgcccactgt
     2641 ctcctccggc gcgctactcg ataagggctc ggatcgtcgg tttcggacgc tcgtcaacga
     2701 tctcttcacg atcgcaagcc gcatggagat cgttcgtacg catttgggcc gacgcatggg
     2761 actcagcgga ccgcaataca gcgttctggt cgcggtcgct catctacagg gcgaacgcgg
     2821 cgtcagggtc ggcacggtcg cccaggccat gcacgtgtcg agtgccttca tcgcgtcgga
     2881 gactggcaag atggcgcgcc acggactgct gctcaagcgg cccaatccgc aagaccgccg
     2941 cggcgtgctg ctcacgttgg cgcccgccgg ccggctcaag atcgaccgcc tcagtgccga
     3001 aatccgtgcg atcaacgaca tgttcttcgg tgcgctcgat ggcgcatctt ttgctgcttt
     3061 gtgcgccgcc gcgcgggcgc tggtgaaagg ctccggcaaa gccgtgcatt atgtgaccgc
     3121 gctcgagagc gagccacggt cagctctgag tgcggccgaa tgagagtcga gggtgatatg
     3181 ccgccggaat gatcaagtgc ggcgcgagaa tgtccttgtg gtatttcgcc gatatgtcaa
     3241 cgttgagcgc ggctctcggt gccgggcgag aatagtatcg agaaatagta tcgagaatag
     3301 tgtgatgtcc tatttcgtcg ggtatgcggt ttggccgccg atctggacgc gtgcgcctgc
     3361 tggtgcggtg ctgcgcgcgg tgatttttac cgcgtcaaga cattcagcgg cgaagtgacg
     3421 catgaggcca tgatcggcaa ggtagtgctc tgatcgatgg caaggagcga cccccgggcg
     3481 cgagatcgag ggcattgcca ttttctatca gcagatcgta aacggcctga tgctcggcgc
     3541 catctacgcg atggtcgccg ttgcattgac gctcagcgtc ggcgttttga gatttctcaa
     3601 cttcagcatt cccggtctgt tcatgatcgg cgggatggcg acatgggtgt tggtgcgtaa
     3661 cgggatgccg tggccgctcg cagctggcgc tgccctcatc gcgggggcga tggcctccct
     3721 cgtggtggag cgtttcactt ggcgctggat gcgtaccgct caacatttcg ttccgctggt
     3781 gagctcgatg gcatttctgc tgctgttcga gcacttggcg gtgatctact ggggatcaga
     3841 gttgcgcacc ttgccggcgc tgttcggcaa tgctgactgg cgcattgcgg gcctcgtcat
     3901 cagcctgccg cagctcgccg gcctgctttg ctcgatcgcg ctaatctggg cgttgactct
     3961 ggtactggcg cgcacccgtc tcggtcgcgg cctgcgcacg atcgccgagg attccgatac
     4021 cgcgctgctg ctcggcgtcg acatcaaccg catcgtgccg gtggtgttcc tcatcaccgg
     4081 cctttttgcc gcgttcgccg gcttgatctt tgcgttgaat tatcgccagg ttcaaccatt
     4141 catgggcgaa gtggtgggtc tcaaaggtat ctcggccatg atcgtcggcg gtatgggcaa
     4201 cgtttggggc tcgattgccg gcgggctgat catcggtctg gtggaggtga tgtcgatcgg
     4261 ttatttcggc gctgatttcg tcgacatcgc ggtctacgaa ctgctgctgc tcatcctgtt
     4321 catccgtccg accggattgt tcgcgggcgc aatcgccggg caggcgcgcg catgagcgga
     4381 tatctggaag gcgtcctggt tctactcgcg gtgaacgtgg tcttcgccta tggcggtttc
//
LOCUS       DATLJT010000592         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3022437,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000592 DATLJT010000000
VERSION     DATLJT010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022437"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VM910_13810"
     CDS             <1..145
                     /locus_tag="VM910_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63643.1"
                     /translation="ILKGEKPADLPVQAPTKYETVINLKTAKALGLTVPDKLLATADE
                     VIE"
     gene            389..1150
                     /locus_tag="VM910_13815"
     CDS             389..1150
                     /locus_tag="VM910_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI63644.1"
                     /translation="MPAVILVHGSGGIGGNVDRWSQELNSFGLATFVTDSFTARGIQS
                     TVANQALLGRLNMILDSYRALGVLAAHPRIDPKKIVLMGFSRGGQATLYASLKRFEQM
                     YGPEGATFAAYAPFYAPCFTTYIGDEDVSDKPIRLFHGTADNYVPVAPCRAYVERLRK
                     AGKDVMLTEYPDANHAFDNPLLKVGPAPQAQTTRRCTMTEEPAGTIINAVTKQPFTME
                     DPCVERGPNLGYNAAATAAATQAVKEFLQVTLKLN"
     gene            1473..>3074
                     /locus_tag="VM910_13820"
     CDS             1473..>3074
                     /locus_tag="VM910_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI63645.1"
                     /translation="MARKGAKSRRRITGLRSKTTKARTHVDRLRAANADLKKKLAEAL
                     EQQAATSEVLGVISRSPGELERVFQAMLENATRICGAKFGAMYLREGDAFRIVAMHNA
                     PPAFAKSRRLQPLVRPGPGTGLYRVASTKQVVHIADIRAEQPYLEREPSSVTMVERAR
                     ARTLLVVPMLKDDELIGNINIYRQEVRPFTDKQIELVKNFAAQAVIAIENTRLLNELR
                     QRTDDLSEALEQQTATSEVLQVISSSPGELEPVFQTMLANAVRICEAKIGSLYIREGD
                     AFRTVATHNAPPAYVEARTRELIRPPPDTMLGRLAATKQVVHIADITTIASYLERNPF
                     MVAAVELGGYRTALGIPMLKDNELIGAITINRQEVCPFTDKQIDLLRGFANQAVIAIE
                     NTRLLNELRESLQQQTATADVLKVISRSTFDLQAVLDTLVESAARLCEAEMAHITRRK
                     DGAFFSIATYGLPPELSDYFKRVPHPLSRGSIVGRVILERTTIQVLDVLADPEYTQIE
                     IQKKVGQRTVLGVPLLREGQPIGVII"
BASE COUNT          656 a          956 c          894 g          568 t
ORIGIN      
        1 catcctcaag ggcgagaagc ccgccgacct accggtgcag gcaccgacca agtacgaaac
       61 ggtgatcaac ctgaagaccg ccaaggcact cgggctcacc gtgccggaca aactgctcgc
      121 caccgccgac gaggtgatcg aataggagag aacacatggg aggggatccg tcatgagcac
      181 ccgttttcga ctggcgattt ttggcatcac ttgtctcggc ttcatcggtc tatgtgcgcc
      241 catcagcagc gcgcaagtgg cgcgaaccga gctgcatgcg atcccgacgc tcacgctgtc
      301 tgaccaccaa ttcctaatcg gcgacaaaaa cggcgcgccg gtcaccatcg cgggagcgtt
      361 gcgggttcca cgtttgggaa cggaccggtt gccggccgtc attctggtac acggctcggg
      421 tggaattggt ggcaacgtcg atcgctggtc gcaggaactc aacagctttg gactagcaac
      481 tttcgtcacc gatagcttca ctgcccgcgg catacagagc acggttgcca atcaagcgct
      541 gctcggccgc ttgaacatga tcctcgattc ctatcgcgcc ctaggtgtat tggccgcaca
      601 tccacgcatc gacccgaaga aaatcgttct gatgggattc tcacgcgggg gccaggcaac
      661 cctttacgcg agcctcaagc gctttgagca gatgtatggc cctgaaggcg caacatttgc
      721 cgcctatgca cccttctacg cgccgtgttt cacgacctat attggagatg aggacgtaag
      781 tgacaaaccc atccgtctct ttcacgggac cgctgacaac tacgttccag tggcgccctg
      841 ccgcgcgtat gtcgagcgcc tgcgcaaggc cggcaaggac gtgatgctga ccgagtaccc
      901 tgatgcaaac cacgccttcg acaatccatt gctcaaggta ggaccagcac cgcaggcgca
      961 gacgacccgc cgctgcacga tgacagaaga gcccgcgggc accatcatca atgcggtgac
     1021 aaagcaaccg ttcaccatgg aggacccttg cgtcgagcgt ggaccgaatc tcggctacaa
     1081 cgccgcggcg accgcggcgg ccacgcaagc ggtgaaggaa tttctgcaag tcacgttgaa
     1141 actgaactga acccttacat cgtcattgcg gtgcgcgggc cccagcgcac acccggcgag
     1201 gacgcctggc cccgaagtgc cgtcggcgct gctcgcgcat gccgacaagg tgatcgaata
     1261 ggctgtaatt tgctgcactg cataatgtcc gcttttggag tgaagcggac atgtcgggca
     1321 ctgcgggagc gtgccgatga gacgctgatg gacccttagc ggtcataggg tggattgaaa
     1381 tcctgcaatg cagcagtctg ccacatatag gtgtgctatc tttcggccgg gaagtcgccg
     1441 cagccgctga ccccacttgc cgcgaggtga tcttggctcg caaaggggcg aagagccgaa
     1501 gacgtattac tggtcttcgt tcgaagacga cgaaggcgag aacccacgtt gaccgccttc
     1561 gtgcagccaa cgccgacctg aaaaagaagc tcgccgaggc gttggagcag caggcggcaa
     1621 cgtcggaagt cctcggcgtc atttcgcgct cgcccggcga gctagagcgc gtgttccagg
     1681 ccatgctgga gaacgccacc cgcatttgcg gggccaagtt cggcgcgatg tatctgcgtg
     1741 aaggtgacgc cttccgcata gtcgcgatgc ataacgcgcc gcctgctttt gctaaatccc
     1801 gacggcttca accgttggtc cgtccgggcc cagggacagg tctttaccgc gtcgcgagca
     1861 caaagcaggt tgttcacatc gccgatatca gagctgaaca gccgtatctg gagcgcgaac
     1921 cttcatccgt cacgatggtt gagcgcgccc gagctcgcac gctcctcgtc gtcccgatgc
     1981 ttaaggacga cgaactgatc ggcaatatca atatctatcg gcaggaggtc cgccccttca
     2041 ctgacaagca gatcgagctg gtcaagaact ttgctgccca agcggtcatc gcgatcgaga
     2101 acacccgcct cctcaacgag ctgcgtcaac gcaccgacga tctgagcgag gcactggagc
     2161 agcagacggc aacatctgag gtgctacagg tcatctcgag ctcacctggc gagctagagc
     2221 cggtcttcca gaccatgctg gcgaatgcgg tgcgcatctg cgaggcaaag atcggctcgc
     2281 tgtacatccg cgagggagat gccttccgca cggttgctac tcacaatgcc ccgcctgcat
     2341 atgtcgaggc gcggacgcgc gaactgatac gaccgccccc ggatacgatg ctcgggcgtc
     2401 tcgccgccac gaagcaggta gtccacattg ccgacatcac gacgatagcg tcctatctcg
     2461 aacgcaatcc ctttatggtt gccgcggtcg agcttggcgg ttatcggaca gcgctcggga
     2521 ttcccatgct caaggacaac gagctaattg gcgcgatcac catcaatcgc caggaggtgt
     2581 gcccattcac cgacaagcag atcgatctgc tgaggggctt cgccaatcag gccgttatcg
     2641 ccatcgagaa cacccggctg ctcaacgagc tgcgcgaatc gctgcagcag cagaccgcca
     2701 cggcggacgt acttaaggtg atcagccgct ccacctttga cctgcaggcc gtgctcgaca
     2761 cgctggtcga gtcggctgcg cgcctgtgcg aagcagaaat ggcacatatc acccgtcgca
     2821 aggatggggc cttcttctca atcgccacct atggcctccc gccggagctg agcgactatt
     2881 tcaaacgcgt tccgcacccg ctttcgcgcg gaagcatcgt cggacgagtg attctggaac
     2941 gcacgaccat tcaagtgctc gatgtgttgg ctgatccgga atacacccag atcgaaatcc
     3001 agaagaaagt cggccagcgt actgtgctcg gggtgcccct gctgcgcgaa ggccaaccga
     3061 tcggcgtgat catc
//
LOCUS       DATLJT010000593         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1414158,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000593 DATLJT010000000
VERSION     DATLJT010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414158"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1380)
                     /locus_tag="VM910_13825"
     CDS             complement(442..1380)
                     /locus_tag="VM910_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI63646.1"
                     /translation="MADEREVNSREFMPLDWRRFQSEPKSFRSRFAYAPVGTMRVSRV
                     ADTQATQFARRPPGIDTYCVSLMARGTSQVVQPMSSEPAVGNTQTGLIFGGEPGTRFE
                     VSGSSVRFHLWVPGRRLRERLEVLLDGQKVKSFAFQPAFDQTRGPGATIRNLLNFLFV
                     ELGRSDSLMANEIATRTLEDNLALCLLLGLRHSWTERLQQQGATAAPGNVRRAEEFMR
                     ANVSTPLSIADIAQAAGCSVRALQMAFDRFRGITPMAALRRIRLEEARTAILRDGRTE
                     SIVRTAADHGFSNPSRFAQLFRRTYGVYPSQAVRAR"
     gene            complement(1696..>2481)
                     /locus_tag="VM910_13830"
     CDS             complement(1696..>2481)
                     /locus_tag="VM910_13830"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI63647.1"
                     /translation="SFMERGASRLLQPRAREAVMGDSATGLVFTAEPGTRYASSDDSV
                     RLYLWFSGKLVRRRLEVLLDGQRADSVAFQAAFDQTRGPGATIRHMIDFLFVEWARSD
                     SLLANEIATRTFEDNLILYLLLGLRHSHTERLQQQRAAAAPGDVKRAEEFMRANTGAP
                     LTIVEIAQAAGCSVRALQVAFHQFRGTTPMAALRRIRLEDARTEILRARHTESIARIA
                     ADHGFSNPSRFAQLFRRTYGTYPSTALRTRSGLDQEGEPFWDN"
BASE COUNT          478 a          762 c          769 g          472 t
ORIGIN      
        1 aggaacagct gcaccgttcc cgccgccagc atgacgccgc ggtcgttgcg gtatgcctcc
       61 gagaagccca gtgtttcata gaacgcgatc gatcttgcga gatcgctcgc gcagatgccg
      121 atattgtcga tgcctcgcac catgctcgac ctttctgacc gacggccgtc agcaaccgcc
      181 gttttctttc aatgttcgac ggaggctgaa gctaatcgag gcactgagca cgatgcgata
      241 cgcaatgcgc agccggcagc cagaaaacgc aatcttttat ggttgggctg aatttgacga
      301 atgtgagctg aattaaccgc cgaaacaggg gagacagcgt ggccggcgtg gtaggcgcaa
      361 acaggatgag gctaaattgc tattcggacc gtgtcccagt gctaatcatc gcttatgccg
      421 ttctcaggac cccgcgcgtc gttaccgagc tcgcacggcc tgtgaagggt agacgccgta
      481 tgtgcgtcgg aagagctggg caaatcggct cgggttgctg aagccatggt cggcggcagt
      541 ccggacaatc gactctgtgc gaccgtcgcg caggattgcg gttcgggctt cttcgaggcg
      601 gatccgtcgc aaggccgcca ttggcgtaat cccccggaaa cggtcgaacg ccatctgcaa
      661 agcgcgaacg ctgcatccgg cagcctgcgc aatatcggcg attgacagcg gcgtggacac
      721 attggcgcgc atgaattcct cggctctgcg cacgttgccg ggtgcggcgg tcgcaccttg
      781 ctgctgcaaa cgctccgtcc agctgtgccg caggcccagc agcaggcaga gcgccagatt
      841 gtcctctaag gtgcgtgtcg cgatctcgtt agccatcagc gagtcggaac gccccaactc
      901 gacaaacagg aaattgagca gattgcggat agtggctcct gggccgcgcg tttgatcgaa
      961 agccggctgg aacgcgaaag atttgacctt ttgtccatcc agcaggacct ccaacctttc
     1021 gcggaggcgt ctcccaggaa cccaaagatg gaagcgtacg ctgctgccgc tgacctcgaa
     1081 cctggttccg ggctcgccgc cgaagatcag gccggtctgc gtattcccga ccgccggttc
     1141 actcgacatg ggttggacga cctggctcgt gccccgcgcc ataagggaga cgcaataggt
     1201 atcaatgccg ggcggtcgtc tggcgaactg cgtggcctgg gtgtcggcga cccgtgagac
     1261 acgcatggtc ccgaccggag cgtacgcaaa tcttgaacgg aagctctttg gttcggactg
     1321 aaagcgccgc cagtcgagtg gcatgaattc gcgcgagttg acctcgcgct catcggccaa
     1381 ggccttctcc tgcggtggaa ggctctccca aatcaggcgg ctggtctgag gcaggtcggc
     1441 cacctcgatc gtcgtgaaat tgccaaacat gccgctccga actcccgccc ctcctcttca
     1501 gttagcaact tgcggcagct tcgcgacggc cgctttcgcc ggcagatccc ccgcgcgtgt
     1561 gactcggtct gatctgcgtt gagcctaatc ggggcgccgg atggcctgcg atacagaaag
     1621 cgcaggcaac agacagaaaa cgcaaatttt ggcgatcatg ttttagaccg atgagatcct
     1681 taggccgccg catcgtcaat tgtcccaaaa aggctcgcct tcctgatcga ggccactgcg
     1741 tgtccgtaaa gccgtggagg gataggttcc gtatgtgcgt ctaaagagct gtgcaaaccg
     1801 gctcgggttg ctgaagccgt ggtcggcggc aattcgggcg attgattcgg tgtggcgggc
     1861 gcgcaggatc tcggttcggg catcctcgag ccggatccgc cgcaaggccg ccattggcgt
     1921 cgtgccccgg aactggtgga acgccacctg cagggcgcgg acgctgcacc ccgccgcctg
     1981 cgcgatctcg acgattgtca gcggcgcacc ggtattcgcg cgcatgaatt cctcggctct
     2041 cttgacatcg ccgggggctg cggccgctct ctgctgttgc agacgctccg tgtggctgtg
     2101 ccgcaagccc agcagcaggt agaggatcaa gttgtcctcg aaggtccgcg tcgcgatctc
     2161 atttgccagc agcgagtcgg aacgcgccca ctccacgaac aggaaatcga tcatatgacg
     2221 gatagtcgct cccgggccgc gcgtctgatc gaaagctgcc tggaaggcaa cagagtcggc
     2281 cctttgtccg tccaacagga cttctagccg ccggcggacg agcttgcccg aaaaccagag
     2341 ataaagccgg acgctgtcat cactcgaggc gtagcgggtg ccgggctcgg cggtgaaaac
     2401 caagccggtc gccgagtctc ccatcaccgc ttcacgcgcc cgcggctgta acagccggct
     2461 cgcgccccgt tccatgaacg a
//
LOCUS       DATLJT010000594         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3711942,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000594 DATLJT010000000
VERSION     DATLJT010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3711942"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1024)
                     /locus_tag="VM910_13835"
     CDS             complement(146..1024)
                     /locus_tag="VM910_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI63648.1"
                     /translation="MSASLMRQPLMLALLFLPSGCLVGVLLIYPLVNGVWLGFTDASP
                     LNRSLHFVGLANFIDLLTDLKFWEVLWNSAFMIVTSIVLSTLLGFVVALMLTSGIRLV
                     GLFRTAVFQVWLVPWIAVAILWGWIFNSDYGIVNFVLIEVGVLAHPQKWLADPLLAQL
                     VIISAFTWRQIPFMMVVALAALQGIPGEVIEAAAIDGAGYARQLVHIVLPLVRNVMMV
                     VALLQAVRLFQEITLPWVLTHGGPINATTTLSLYAYKIAFERWDFGMASAVGTIWLLL
                     LAGFALIYVRLLVARR"
     gene            1226..>1585
                     /locus_tag="VM910_13840"
     CDS             1226..>1585
                     /locus_tag="VM910_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HVI63649.1"
                     /translation="MARSQSRLTAEIDFNRNGKQHGFIRLPYSVHRSAYGWIPIPVAC
                     IKNDAGPRILLMAGNHGDEYEGQVALGKLIRDLEPDEVSGRIIILPSANFPAAMAGMR
                     TSPLDGGNLNRSFPGDPY"
BASE COUNT          363 a          515 c          472 g          235 t
ORIGIN      
        1 gccgtcgatg ccatccaggc gatcgggagc aggagcaccg cgatattggc ggagacgagc
       61 agatacaccc ccgtgcgagc cgcttgccgc cgcaatcgcc gcaacactgg cgccgggaac
      121 tgtcggacgg ctgggaccgg cgccatcatc gccgtgccac cagcaggcgg acatagatca
      181 gcgcaaagcc ggccagcagc aacagccaaa tcgtgccgac cgccgacgcc atcccgaaat
      241 cccagcgctc gaatgcaatc ttataggcgt agaggctcag ggtcgtcgtc gcgttgatcg
      301 ggccgccatg cgtcagcacc cagggcaacg tgatctcctg gaacaaacgg acggcctgca
      361 gcagcgccac caccatcatg acgttgcgga ccaagggcaa cacgatatgc acgagctgcc
      421 gggcatagcc ggcgccgtcg attgctgccg cttcgataac ctcgccggga atgccttgca
      481 gcgcggcgag cgcgacgacc atcatgaacg ggatttgccg ccaggtgaac gccgagatga
      541 tgaccaactg cgccagcagc gggtcggcga gccatttctg cggatgggcc aagacgccga
      601 cctcgatcag cacaaaattc acgatcccgt agtcgctatt gaagatccag ccccacagga
      661 tcgcgacggc aatccacggc accagccaga cctggaaaac cgcggtgcgg aacaggccga
      721 ccaagcggat gccgctggtc agcatcagag cgacgacaaa gccgagcagc gtcgacaaca
      781 cgatcgaggt gacgatcatg aacgcactgt tccacaacac ctcccaaaac ttcagatcgg
      841 tcaggagatc gatgaagttc gcgagaccga cgaagtgcag gctgcggttg agcggcgacg
      901 cgtctgtaaa gccgagccag acgccgttga ccagcggata gatcaacaat acgccgacca
      961 gacagccgga gggcaggaac agcagcgcca gcataagagg ctgtcgcatc aggctggcag
     1021 acatcgatgc cggctcgctt caatacacgc gccatccttg tcgaacgtcc gggccagggt
     1081 gcgcaagccc gccgcgatgg ctagccggag ctaccagact agaggcgcaa tctttgacca
     1141 acccgcgacg ctgtttggct aaagacgcaa gcacaccgct ttcatgctat caattggcga
     1201 acttcgcggc gcacaggagc cattgatggc ccgctctcag agccgtctca ccgccgaaat
     1261 cgatttcaat cgtaacggca aacagcatgg ctttatccga ctgccctatt cggtgcaccg
     1321 ctcggcctat gggtggatcc cgatcccggt cgcttgcatc aagaatgacg cggggccgcg
     1381 aatcctcctg atggccggca atcacggcga cgagtacgaa gggcaggtcg cgctcggcaa
     1441 gctgatccgc gacctcgaac ctgacgaggt cagcggccgc attatcatcc tgccctcggc
     1501 gaatttcccg gcggcgatgg cagggatgcg cacctcgccg ctcgacggag gcaacctcaa
     1561 ccgaagcttt ccgggcgatc cctat
//
LOCUS       DATLJT010000595         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_724418,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000595 DATLJT010000000
VERSION     DATLJT010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724418"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..245
                     /locus_tag="VM910_13845"
     CDS             18..245
                     /locus_tag="VM910_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HVI63650.1"
                     /translation="MILESVITCPHCATAKSEVMPTDACQFFYTCTGCGATLRPKPGD
                     CCVFCSYGSVPCPPIQAERSGETGAASCCAR"
     gene            complement(167..340)
                     /locus_tag="VM910_13850"
     CDS             complement(167..340)
                     /locus_tag="VM910_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63651.1"
                     /translation="MTAFGIHHANALVRGLLSQSCELWTTSFAMMGYLAQHEAAPVSP
                     ERSAWIGGQGTEP"
     gene            761..2104
                     /locus_tag="VM910_13855"
     CDS             761..2104
                     /locus_tag="VM910_13855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI63652.1"
                     /translation="MKTYDLVIIGSGTAAQVASSRVRAAGWSVALVDHRPFGGTCALR
                     GCDPKKMLISGAEVIDAKTRMRGHGVGGEARIDWRDLMMFKRSFTDAVPRNRERDFAE
                     NGIDAFRGLARFTSRDVVAVGGLELKARYILIAAGARPVPLNIPGQEHAVTSEGFLEL
                     DRLPARLVLVGGGYIAAEFSHITARAGIHVTVLQRAQQMLPAFDPDLVGWLMEKFGEL
                     GIDVRTRATVQRIDKTAGGFIVHASTDGQEQEVAADLVVHAAGREPDLAALDLAAGGV
                     ATENGRVKLNEFLQSVSNPKVYAAGDAASSGPPLTPVSSHDGKVVAQNLLDGNQHRPD
                     YRGVPSVAFTIPPIAAVGFSEAKARAKGIKFRMHSEKASDWYTARRLNESVYGFKTLV
                     EEDTDRILGAHLVGPHAEEVINLFGLAIRHGLTTKDLKTTIFAYPTGASDIGYML"
     gene            2387..2530
                     /locus_tag="VM910_13860"
     CDS             2387..2530
                     /locus_tag="VM910_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63653.1"
                     /translation="MTVTTGRRELLAAVGGATLAWPLWSPDASAVFGPRASRSCPPLS
                     PGN"
     gene            2579..3222
                     /locus_tag="VM910_13865"
                     /pseudo
     CDS             2579..3222
                     /locus_tag="VM910_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
BASE COUNT          611 a          949 c         1009 g          653 t
ORIGIN      
        1 atgctttagg aagaacgatg atccttgagt ctgtcatcac ctgtccgcat tgcgccaccg
       61 cgaaatcgga ggtcatgcca accgatgcct gccagttttt ctatacatgc accggctgcg
      121 gcgcgacact gcggccgaag ccgggcgatt gttgcgtgtt ctgttcctat ggctcggttc
      181 cctgcccgcc gatccaggcc gagcgttcgg gtgagacggg cgccgcttcg tgctgtgcca
      241 gatagcccat catggcaaac gatgtcgtcc aaagctcgca cgactggctc agcagccccc
      301 gcactagcgc gttggcatgg tggattccga aagcggtcat cgttgcggct cttttccttc
      361 ccgcgcccgc gcgagccggg atgtggatca tcgcgctcat ttggatgggt acagcgtgca
      421 ttctgaattc aaggcggtgc ggacgaaccc actgccgcta tacaggccct tattatctcg
      481 cgatgatcgc accggtggtc gtgcttgctt cgggcgccgt ttccgtcgac gacgtctatg
      541 catggctttc attggtggtt ctcattcttg gcggaagatc atttggtggg ccacggaaag
      601 ggcgtgggga aagttctcgc ggcgcgacga tagcttttga tcatcaggtc aatagccgaa
      661 aactaatgta catacttcgg tactcttcct cataatcgtc ccgcgagggc catgcagttc
      721 attggacgtg tgatcacttg ccttcggagg agaccacgtc atgaagacct acgatctcgt
      781 gatcatcggc tctggcactg ccgcgcaggt ggcaagctcg cgagtgcgtg ccgcagggtg
      841 gtctgtagca ttggtcgatc atcgtccttt cggcgggact tgcgccctgc gcggctgcga
      901 tccgaagaaa atgctgatta gcggtgcgga ggtgatcgac gcgaagactc gtatgcgcgg
      961 gcatggcgtc gggggcgagg cgcggattga ttggcgcgac ttaatgatgt ttaaacgcag
     1021 cttcaccgat gctgtgcccc gcaatcggga aagagatttt gccgaaaacg gcatcgacgc
     1081 ctttcgtggg cttgcccgct tcaccagtcg agatgtggtt gccgtcggag gcctggaact
     1141 caaggctcga tacatcttga tcgctgctgg tgcgcggccg gtaccgctga acatccctgg
     1201 ccaggagcat gctgtcacta gcgaaggatt tcttgagctt gatcggctac ccgcgcgcct
     1261 cgtcctagtc ggcggcggct acatcgccgc cgaattctcg cacatcacag ctcgggccgg
     1321 tatccatgtc accgtgcttc agcgcgccca gcagatgctg ccggcattcg atccagatct
     1381 ggtgggttgg ctcatggaga agttcggcga actcggcatc gacgtgcgca cacgggcgac
     1441 cgtgcaacgg atcgataaaa cggctggcgg cttcattgtg catgcttcaa cggacggaca
     1501 ggaacaggag gtggctgcag atcttgtcgt gcacgccgcc ggacgcgagc ccgatctggc
     1561 ggcgctcgat cttgctgccg gcggtgtcgc aaccgaaaac ggtcgagtga agctcaacga
     1621 gttcctgcag agtgtatcaa accccaaggt ttatgccgca ggtgacgccg caagcagcgg
     1681 cccgccgctc acgccggtat cgagccacga tggcaaagtc gtcgcgcaaa accttctcga
     1741 cggcaaccag caccggccgg actaccgggg cgttccgagc gtcgcattca caattccgcc
     1801 aattgccgcg gttgggttca gcgaggcgaa ggcgcgcgcg aaggggatca aatttcgaat
     1861 gcactcggag aaggcttctg actggtacac agcccgacga ctcaacgaat cggtctacgg
     1921 cttcaagacc ctcgtcgagg aggacaccga tcgcatcttg ggcgctcacc tcgtcgggcc
     1981 ccatgccgaa gaggtcatca atttgttcgg cctcgcgatc cgccacgggc tgacgacgaa
     2041 agatctgaag acgacgatat ttgcttatcc gacgggggcc tctgacatag ggtacatgct
     2101 gtagctgctt gcgggaaaac gagtctccga aggacccgaa gctacccatg aggcgatgcg
     2161 gagggcagca ttaatgtcgc cttttggcac accgcgtcgt ttcgctgcgc tggcgaagtt
     2221 tgaaagcgat cggagtaaaa gcggacatga agaccagttg gcgaacttcg gaaggtggcg
     2281 gcgtcgcaca caccgtggga gctgtgactg ccctccttga acgtgaagac tacacgcgtg
     2341 tggcatggta tcgttgcctt tacgcaccct cgcgcgggag gggcgtatga cagtcaccac
     2401 cggacggcgg gaattgctgg ccgcagttgg cggcgcgacg ctcgcgtggc cactttggtc
     2461 gccggacgcg tcggccgtat ttggtcctcg cgcgagtcga tcctgcccgc cgctctcgcc
     2521 cggcaactga gcgcgtagct gcggatgcgg gcgtcaagct ggttcgagag gaaatgccat
     2581 gtctgcttca tcggccccga cgtcggatgc ctcgaagaca ccttcccgtg cgatcccgaa
     2641 ggttctgctc ggccagaagg ccctggtgac gggcgcaaat tcaggcatcg gtaaggccac
     2701 cgcaattgcg ctggggcagg ccggcgccga cgtggtggtg aattatgtca gcggcgagga
     2761 tcacgcggac gcggtggtgg gcgagatccg tcgatctgga gcgaaagcat acgcgcacaa
     2821 ggcggacgtc tctcaggaag agcaagtcca ggagatgttc cgccgcatgc tggaagagct
     2881 cggcacgccc tcctcgtcaa caatgccggg cttcagcgcg acgccccatt ccatgaaatg
     2941 aactcttgtg cagtggaaca cagtcatcgg catcaacttg acgggccact tcctgtgcgc
     3001 tcgcgaggcc gttcgccact ttctgcagcg cggcgtcgtc tctcgggttt cctgcgccgc
     3061 cggcaagatc atattcatga gctcagtgca cgaaatcatt ccgtgggccg ggcacgccaa
     3121 ttacgcggca tcgaaaggcg gcgtcatgct gctgatgaaa agcctcgccc aggagcttgc
     3181 ccctcgtcgc attcgcgtca acagcatcgc gcccggggcc at
//
LOCUS       DATLJT010000596         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4401091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000596 DATLJT010000000
VERSION     DATLJT010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VM910_13870"
     CDS             complement(<1..212)
                     /locus_tag="VM910_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63654.1"
                     /translation="MAKRAITAKNMQGSLIESLRQRSRFAAVDTVDASLRARCFASLE
                     CIPTLTLLCHFIDAIKAGDFARAESVR"
     gene            complement(449..1159)
                     /locus_tag="VM910_13875"
     CDS             complement(449..1159)
                     /locus_tag="VM910_13875"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HVI63655.1"
                     /translation="MSRGVKDILVTGITATTLLTSVASASAQNIFEALFGRLWNSPVT
                     SYADPNASSRSPEATRSEGGLAYCVRLCDGRYFPIQRYSGATSAQTCSSMCPASATKV
                     YNGNGIDHAVAPDGKRYSELATAFTYRKKIVPDCTCNGRDAFGLVNTTVADDPTLRPG
                     DIVATNAGLMAYNGGPNGSTFTPISTSAGLSADLRRQLTETKIEPAAEGPELLPPLRQ
                     AEAPPARSSTNKRAQVDR"
     gene            complement(1458..2363)
                     /locus_tag="VM910_13880"
     CDS             complement(1458..2363)
                     /locus_tag="VM910_13880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63656.1"
                     /translation="MPVIGFLGATSFDNNVDRLRVFRQGLKESGYIEGENVAIEYRWA
                     EGQYERLPALAGDLVRREVSVIASAEGAITAFAAKAATATIPVVFVISDDPVRLGLVA
                     SFARPGGNLTGINFVSAELAAKRLELLHELVPGAKRVALLVNPADTTNTETMLREVGP
                     AASAIGWQIQVLNADTSGAIDAAFSTFERERPDALFVGSSAFYTSRRVQLVQLTARHA
                     IPAAYVGRQYTEIGGLMSYGANLTDAWRQLGVYTGRVLKGAKPADLPVVQANKFELVI
                     NHQTAKMLGLAVPDKLLVAADEVIE"
     gene            complement(2441..>2767)
                     /locus_tag="VM910_13885"
     CDS             complement(2441..>2767)
                     /locus_tag="VM910_13885"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63657.1"
                     /translation="ALFVAADAFFNSRPGRLATFATHYRIPAAYSVREAVEAGGLMSY
                     GADRADGSRQVGVYTGQILKGAKPADLPVLQSSKFEFVINAQTARLLGIEVPNSLQLL
                     ADEMIE"
BASE COUNT          538 a          842 c          850 g          537 t
ORIGIN      
        1 cgaacacttt cggcgcgagc gaagtcgccg gccttgatcg cgtcgatgaa gtgacaaaga
       61 agcgtaaggg tcggtatgca ctccaaactc gcgaagcagc gcgcgcgtaa actcgcgtcg
      121 acagtgtcca ctgcagcaaa acgtgaacgc tgccgtagag attcgatcaa ggacccctgc
      181 atgttcttgg ctgtgatggc gcgcttcgcc atgttgttgc gaacctcgcg gacagcgaga
      241 acggtccggt cacgaacgac cgaggcttcc ctccgcaact cgtttggccc aagacacagc
      301 tcttccagtg cggcaatttc gtcttcgccc ttcgtgatcg cgtaatcaac gcgggcgcaa
      361 tcagcgagtt ggcgtcgctc aatctcaagc acttttggta gcagcgtggt catacctggc
      421 tacccggcaa ggaaaagcat ccgaatgcct acctgtcgac ctgagcgcgc ttattcgtgc
      481 tactgcgggc gggcggcgcc tccgcctgcc ggaggggcgg cagcagttca ggtccctccg
      541 cggccggctc gatcttggtt tcagtgagct ggcgccgtag gtctgctgag agtcccgcag
      601 aggtgctgat gggagtgaag gttgagccgt ttggcccgcc gttataggcc atcagaccag
      661 cattggtcgc gacgatgtcg ccgggacgca atgtcggatc atcagcaacg gttgtgttga
      721 cgaggccgaa ggcgtccctg ccgttgcagg tgcagtcggg gacgatcttc ttgcggtacg
      781 tgaacgcggt ggcaagctct gagtagcgct tgccgtctgg cgcgaccgcg tggtcgatgc
      841 cgttgccgtt gtagaccttg gttgcgcttg ccggacacat ggaactgcag gtctgcgccg
      901 aagttgcccc gctataacgt tgaattggga agtaacgtcc gtcgcatagg cgcacacaat
      961 aggcgagccc tccctcggaa cgggtcgctt ccgggctgcg cgacgaggcg ttcggatccg
     1021 cataactggt gacgggagaa ttccacaggc ggccgaacag cgcctcgaaa atgttttggg
     1081 cagaggccga agcgacagag gtgagaagcg tcgtggccgt gatgccggtc acgaggatgt
     1141 ctttgacgcc gcggctcaag cgggtctgat gacggatcaa caaagcgaag ggtcccccga
     1201 ctcgcgcgcc gacgatcccc cggcgcacca tctctcaata ccttcggcga cgggcggaaa
     1261 tatgacctga gagtgccgag agtaagacgt tttggagcgc ttcagaacgt gcaaaagttg
     1321 tccgcttttg ggtcagcagc ggacattacc cgacacctag ccgatgtccg ctttgccccc
     1381 gaaagcagac atcacgacct tccctcgata ggtccgcttt gtgccaaacg cagacattcg
     1441 cactgcagca cgttttctta ttcgatcacc tcgtcggcgg cgacgagcag cttgtcgggc
     1501 acagcaagtc cgagcatctt ggcggtctgg tgattgatga cgagctcgaa cttgttcgcc
     1561 tgcacgaccg gtaaatccgc gggctttgcg cccttgagga cgcggccggt gtagacgccc
     1621 aactgacgcc atgcatcggt gaggttggcc ccatagctca tgaggccgcc gatttccgta
     1681 tattgccgtc ccacatatgc ggccgggatc gcgtgccgcg cggtcaactg taccagttga
     1741 acacgccgac tcgtgtaaaa agcactactg cccacgaaga gcgcgtcagg ccgctcgcgc
     1801 tcgaacgtgc tgaaggccgc atcgattgcg ccactggtgt cagcgttcag aacctgtatt
     1861 tgccacccga tggcgcttgc agctgggccc acctctctca acatggtctc ggtgttagtg
     1921 gtatcggccg gattgacgag gagggccaca cgcttggctc ctggtaccag ctcatgcagg
     1981 agttcgagcc gttttgccgc cagctcagcc gagacaaaat tgatacctgt caggttgccg
     2041 cctggccgag cgaaactggc cacaagaccg agcctgacag ggtcatcgct gatgacgaag
     2101 acaaccggga tggtcgcagt ggcggccttg gccgcgaatg ccgtaatcgc cccttccgct
     2161 gaggcgatta cggagacctc tcggcgaacg aggtcgcccg ccagggccgg cagccgttca
     2221 tattgacctt ctgcccagcg gtattcgatc gcgacgttct cgccctcgat atagccgctc
     2281 tccttcaaac cctgacgaaa tacgcgcaga cggtccacgt tattgtcgaa cgatgtagcg
     2341 ccaaggaatc cgatcaccgg catcgccggc tgctgcgcgc gcgccgcgag cggccacccc
     2401 gccgccgcgc cgccgaagag cgtaatgaac tcgcgccgtt tcattcgatc atctcatcgg
     2461 cgagcaattg caacgaattg ggcacctcga tgccgagcag tctcgccgtt tgcgcgttga
     2521 tgacgaactc gaatttgctc gactgcaaaa ccggcaggtc ggcgggcttc gcgcctttga
     2581 ggatttgccc ggtatagacg ccgacctgac gagacccgtc cgcgcgatcg gccccgtagc
     2641 tcatcagccc tccggcttcg acagcctcgc gaaccgaata ggccgcggga atccgatagt
     2701 gcgtcgcgaa cgtcgctagt cggccagggc ggctgttgaa gaaggcatcg gcggcaacaa
     2761 acagggc
//
LOCUS       DATLJT010000597         3728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1873796,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000597 DATLJT010000000
VERSION     DATLJT010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873796"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VM910_13890"
     CDS             complement(<1..1169)
                     /locus_tag="VM910_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVI63658.1"
                     /translation="MKKLSPEAYAIVEGRHADPFRYLGLHKEGDKTIVRAFLPEASNV
                     EAVGEHGETAKLARIHDAGLFAGALPNGSRRYQLRARFGENVVELEDPYRFPPVLSDF
                     DLYLLAEGTHQRLYDKLGAHPLVLDGVDGVAFVVFAPNARRVSLVGDFNYWDARRHPM
                     RVRGAGYWELFIPRAKPGDHYKFDIIGPQGQHLPLKSDPLAFATEVRPKTASIVFDEA
                     TLPRPRATPPGINARNAPISIYEIHLGSWRRKGRNEWLTYGELAEQLPAYVRDLGFTH
                     IEFLPVNEHPFDGSWGYQPTGLFAPTSRFGSPADFAALVDACHAQGLGVILDWVPGHF
                     PDDPHGLGRFDGTALYEHANPQQGRHLDWGTLIYNYGRTEITNFLVSNALFWLER"
     gene            complement(1169..>3728)
                     /locus_tag="VM910_13895"
     CDS             complement(1169..>3728)
                     /locus_tag="VM910_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HVI63659.1"
                     /translation="DAYSKGKVLLAEANQWPEDVQEYFGHGDECHMAYHFPLMPRIYM
                     AIAQEDRFPITDILRQTPDIPVNCQWALFLRNHDELTLEMVTDVERDYLWSTYANDPR
                     ARINLGIRRRLAPLMDNDRRKIELMNSLLMSFPGTPIIYYGDEIGMGDNIYLGDRNGV
                     RTPMQWTLDRNGGFSRADPAKLYAPMIMDPVYGYQAVNVEAQSRSLSSLLSATKRLIS
                     VRKSTLAFGRGSMTFVRPANRAVLAYIREYKGEVILCVANLSRAAQATQLDLSVWKDR
                     IPLEMLGRTRFPPIGELPYMITLAPYGFYWFQLLERGKSEPSSPRAVPEFETLVVPLN
                     STWVSLARTRGVFERDVLPGHLARTRWYPEGAPEAIRPTLVSAVPFCDIGDNRPWLAF
                     FEAKQHGKTDRYVLPMQIEWVRFDREHYNPHAFAAIRQGAREGTLLDVATNPIFIGLL
                     LRNLRQSLTVEENGLRLEFRPTQRFADRLVRQPEHIRSLETERPSSAALVDGDYVVKL
                     YRKLESGIHPAIEIGRFLTDTAGFANAPALLGSVELVEDGDKRTAIAPVHAFVANQGD
                     GWTVSTGYLDRFIDEQRLLAASADANENEEQVLYLRHMSQAGRRIAELHLALASHPEL
                     ADFAAEPIGPDDVERWTSELAARSTRALNLLGQRRDVIRDADRPLVDRVLALRRPLLK
                     QLNTMLPSDIDGLNIRLHGDFRLDQLLIVKDDIFIVGFDGDPRQSLAERRRKAPAARD
                     VAALIRSIDYSVMAALDRAFKGAPDEQGNRLAAALADWSDRAVSAFHTGYREAMTNDR
                     MWPADPHATNTMLNFFLLERIVGEIEDELADRGEWLRIPLNAMLRMLSVPASES"
BASE COUNT          682 a         1109 c         1259 g          678 t
ORIGIN      
        1 tagcgctcga gccagaacag cgcgttcgac acgaggaagt tcgtgatttc ggtgcggccg
       61 taattgtaga tcagcgtgcc ccagtcgaga tggcgtccct gctggggatt ggcgtgctcg
      121 tagagtgcag tgccgtcgaa ccggccgagg ccgtgcggat catcgggaaa gtgcccgggc
      181 acccagtcga gaatcacgcc gaggccttgt gcgtggcagg catcgacgag cgcggcaaag
      241 tcggcgggcg agccgaagcg gctggtcggc gcaaacaggc ccgtcggctg ataaccccac
      301 gagccatcga acgggtgctc gttgacgggc agaaattcga tatgggtgaa accgagatcg
      361 cgcacgtaag cgggaagctg ctccgccagc tcgccatagg tcagccattc attgcgtccc
      421 ttgcggcgcc acgagccgag atggatctca tagatcgaga tcggcgcgtt gcgcgcgttg
      481 atccccggcg gagtcgcgcg cggacgcggc agcgtggcct cgtcgaacac gatcgaagcg
      541 gtcttggggc gcacttcggt ggcaaaggca agcggatcgg atttcagcgg caggtgctgc
      601 ccttggggtc cgatgatgtc gaacttgtag tgatcgcccg gtttggcgcg tgggatgaac
      661 agctcccagt agccggcgcc gcggactcgc atcggatgcc gccgtgcgtc ccagtagttg
      721 aagtcgccga ccaggctgac ccgccgtgca ttgggggcga acaccacaaa ggccacgcca
      781 tcgacgccgt cgagcaccag cggatgcgcg ccgagcttgt cataaagccg ctgatgcgtg
      841 ccctcggcca gaagatacag gtcgaagtcg gacagtaccg gcgggaagcg gtaggggtcc
      901 tcgagttcga cgacattctc gccgaaccgg gcgcggagct gatagcggcg cgagccgttg
      961 ggcagcgcgc cggcaaaaag gccggcgtcg tggatgcgcg caagcttcgc cgtctcgcca
     1021 tgctcgccga cggcttcgac attggaagcc tcgggcagga acgcgcgcac tatggttttg
     1081 tcgccctcct tgtggaggcc gagatagcga aagggatcgg cgtgacggcc ctcgacgatg
     1141 gcataggctt cgggagacag tttcttcatc agctctcgct tgcaggcacg gacagcatac
     1201 gaagcatggc gttcaggggg atgcgcagcc attctccccg atcggccagc tcatcttcga
     1261 tctcgccgac gattctttcc aacaaaaaga agttcagcat ggtgttggtt gcatgcggat
     1321 cggcgggcca catgcgatcg tttgtcatgg cttcgcgata gcccgtgtgg aacgccgaga
     1381 ccgcgcggtc gctccagtcg gcgagcgcag cggccagtcg gttgccttgc tcgtcgggtg
     1441 cccctttgaa tgcgcgatcg agcgccgcca tgacggaata gtcgatcgac cggatcagcg
     1501 cggcaacgtc gcgagccgca ggcgccttgc gccgccgctc ggcgagactc tggcgagggt
     1561 cgccatcgaa gccgacaatg aagatgtcgt ccttgacgat cagaagctgg tcgaggcgga
     1621 aatctccgtg caggcggatg ttcaggccgt cgatatcgga tggcagcata gtgttcaatt
     1681 gcttcagcag gggacggcgc agcgccagca cccggtcaac cagcggtcga tcggcatccc
     1741 tgatgacgtc gcggcgctgg cccaacagat tgagagcgcg cgtggagcgt gccgcgagct
     1801 cgctggtcca acgctccacg tcgtcgggcc ctatcggttc ggctgcaaaa tcggcaagtt
     1861 ccgggtgcga agcaagggcc aggtgcagct cggcgatgcg tcgtccggct tgcgacatgt
     1921 gacgcagata gagaacctgt tcctcgtttt cgttggcatc ggcgctggct gcgagcagcc
     1981 gctgctcgtc gatgaagcgg tcgagatagc ccgtgctgac cgtccagccg tcgccctggt
     2041 tggcgacaaa ggcatggact ggcgcgatcg cggttcgctt gtcgccgtcc tccacgagct
     2101 cgacgcttcc gagcagcgcc ggcgcgttgg caaagcctgc ggtgtcggtc aggaagcggc
     2161 cgatctcgat cgcgggatga atgccggatt ccaacttgcg gtagagcttg acgacatagt
     2221 cgccgtcgac cagcgccgca cttgacggcc gctcggtctc gaggctcctg atgtgctcgg
     2281 gctggcgcac caggcggtcg gcaaatctct gcgtcggtct gaactcgaga cgcaaaccgt
     2341 tttcctcaac ggtcagggat tgtctcagat tgcgcagcaa tagcccgatg aagatcgggt
     2401 tggtcgcaac gtccagcagc gttccttcgc gcgcgccctg ccgaatggcg gcaaacgcgt
     2461 gcggattata gtgctcgcga tcgaagcgca cccattcgat ttgcatcggc agcacgtagc
     2521 ggtcggtttt gccgtgctgc ttggcttcga aaaatgcaag ccaaggccgg ttgtcgccga
     2581 tgtcgcagaa cgggacggcc gacaccagcg tcgggcggat tgcttcgggt gcgccctccg
     2641 gataccagcg cgtgcgcgcc agatgccccg gcagcacgtc gcgctcgaat acgccgcggg
     2701 tgcgggcgag cgacacccag gtcgagttga gcggcaccac cagcgtttcg aactcaggca
     2761 ccgcgcgcgg cgaactcggc tcggatttgc cccgctccag tagctggaac cagtagaagc
     2821 cgtaaggcgc cagcgtgatc atgtaaggca gttcgccgat cggcggaaaa cgcgtacggc
     2881 cgagcatctc gagcggaatg cggtccttcc agacggatag atcgagctgg gttgcctgcg
     2941 ccgcgcgcga caggttggcg acgcagagga ttacctcgcc cttgtattcc ctgatatagg
     3001 caagcacggc ccggttggcc gggcggacga aggtcatgct accgcggccg aaggcgagcg
     3061 tcgatttccg caccgagatc agccgcttgg tagcggacaa cagcgaggac aggctgcgcg
     3121 actgcgcttc gacgttgacc gcctgatagc cgtagacggg atccatgatc atcggcgcat
     3181 agagttttgc ggggtcggcg cgcgagaacc cgccgttgcg atcgagcgtc cattgcatcg
     3241 gcgtgcgcac gccgttgcgg tcgccgagat agatgttgtc gcccatgccg atctcgtcgc
     3301 cgtaatagat gatcggcgtg ccgggaaacg acatcaacag cgagttcatg agctcgatct
     3361 tgcgccggtc attgtccatc agcggcgcga gccgtcgcct gatcccgagg ttgatgcggg
     3421 cgcgcggatc gtttgcatag gtcgaccaca gatagtcgcg ctcgacgtcg gtgaccattt
     3481 ccagcgtcaa ttcgtcatga ttgcgcagga acagcgccca ttggcagttg acggggatgt
     3541 cgggcgtctg gcgcaggatg tcggtgatgg gaaagcgatc ttcctgcgcg atcgccatgt
     3601 agatgcgcgg catcagcggg aaatgatagg ccatatggca ttcgtcgcca tggccgaaat
     3661 attcctgcac gtcctccggc cattgattgg cttcggcgag cagaaccttg cccttggaat
     3721 aggcgtcg
//
LOCUS       DATLJT010000598         1901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3712119,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000598 DATLJT010000000
VERSION     DATLJT010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712119"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VM910_13900"
     CDS             complement(<1..351)
                     /locus_tag="VM910_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI63660.1"
                     /translation="MTVETILRQKGADVATIEPDASIRRAADWLRVKNIGALVVISGE
                     AVLGLISEREVVHAFSRYGETATLMPVKEIMRYGVITVSPDESVSHVMQLMTRHRVRH
                     MPVLRDGKLAGIISI"
     gene            complement(501..1478)
                     /locus_tag="VM910_13905"
     CDS             complement(501..1478)
                     /locus_tag="VM910_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63661.1"
                     /translation="MKLPRRKFLYLAAGVAALPAVSRIARAQAYPTRPVRLIVPVAPA
                     GASDITARLIGQWLSERLGQQFVIDNRPGGGTNIGTEAVVRAPADGYTLLVVGSYNAT
                     NAALYDKLNFNFIRDIAPVAGVFRGPYVMVVNTSVPASTVPEFIAYAKANPGKISMAS
                     SGIGAGAHLAGELFKMMAGIDMVHVPYRGGGPAVTDLIAGQVQVYFASTVASIQYIRA
                     GRLRALAVTAAMRSDALPDIPTVAEFVPGYEVSTWYGVGAPKATPAEIVEKLNTEINA
                     GLAEPKMKARLADLGGDVLALSPAEFGKLIADETEKWGKVIQAANIKPE"
     gene            complement(1515..>1901)
                     /locus_tag="VM910_13910"
     CDS             complement(1515..>1901)
                     /locus_tag="VM910_13910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63662.1"
                     /translation="LAGQVQVGFPTIGAVIEHIRTGKLRALAVTAATRLDLLPDVPAL
                     GEFVSGYEASGWTGIGAPRNTSAEIIETLNKAINAALTDPKVKARLADLGLEPLANSP
                     AEFGNYILQYTEKWGKVVRAANIKGE"
BASE COUNT          343 a          597 c          585 g          376 t
ORIGIN      
        1 gatgctgatg ataccggcga gcttgccgtc gcgcagcaca ggcatgtggc gcacgcgatg
       61 gcgcgtcatc agctgcatca cgtgactgac actctcgtcc ggggacacgg tgatcacgcc
      121 gtatcgcatg atttccttca ccggcatcaa agtggcagtc tcgccgtagc gagaaaaagc
      181 gtgaacgact tcacgttcgg aaataagacc gagaacagcc tccccgctta tcaccacgag
      241 agcaccgatg ttctttacgc gcaaccagtc cgccgctctc ctaatgctgg catccggctc
      301 tattgtcgcg acgtcggcgc ccttctgcct cagaatggtt tcaacggtca cagctgcatc
      361 tcctgagttg tctgctcccc aaagtgattg gcctctaagg gtgagcgtgc acccttccca
      421 atagattggc aacgcggatc gggggatgca acctcgactt tgcttaacgg gagattatgg
      481 aatatcgcgg aatttccgga tcactccggc ttgatgttgg ccgcctggat caccttgccc
      541 cacttctcgg tttcgtcggc gatcagtttc ccaaattcgg cgggcgagag cgcgagcacg
      601 tcgccaccga ggtcggccag ccgcgccttc atcttgggct cggcaaggcc agcgttgatc
      661 tccgtgttga gcttctcgac gatctcggcc ggcgtggcct tcggcgcgcc gacgccgtac
      721 caggtgctca cctcgtagcc cggcacgaac tcagccacgg tcgggatgtc cggtagcgcg
      781 tccgagcgca ttgcggcggt gactgcgagc gcgcgcagcc tgccagccct gatatactga
      841 atcgatgcga cagtgctggc gaagtagacc tgcacctgcc cggcgatgag atcggtgacc
      901 gccggcccgc caccgcgata gggcacgtgg accatgtcga tgccggccat catcttgaaa
      961 agctcgccgg ccagatgagc cccggcgccg atgcccgacg acgccatgct gattttgcct
     1021 ggattggcct tggcgtaggc gatgaactcg ggaaccgtgc tggccggaac cgacgtgttc
     1081 accaccatga cgtagggacc tcggaagacg cccgcgaccg gcgcgatgtc gcggatgaaa
     1141 ttgaagttga gcttgtcgta caacgctgcg ttggtcgcgt tgtagctccc gaccacgagc
     1201 agcgtgtagc cgtctgcagg cgcgcgtacg accgcctcgg tgccgatgtt ggtgccgcca
     1261 cccggcctgt tgtcgatcac gaattgctgg ccgagccgtt ccgacagcca ctgaccgatc
     1321 aggcgcgccg tgatgtcgga cgcgccggca ggcgcgactg gcacgataag acgcaccggc
     1381 cgcgtcggat aggcttgcgc ccgcgcgata cgcgacaccg ccggcagcgc ggcaacgccc
     1441 gcggccagat acaagaattt gcggcgggga agtttcatat ggttcttccc tgaaatgcca
     1501 aacgcgagcc ggatttattc tcccttgatg ttcgcggccc ggaccacttt gccccacttc
     1561 tcggtatatt ggaggatgta gttgccgaac tcggcgggtg agtttgcaag cggctccagg
     1621 ccgaggtcgg caaggcgcgc tttcaccttc ggatcagtga gagcggcgtt aatcgccttg
     1681 ttgagggttt cgatgatctc ggcagatgtg ttcctgggtg cgccgatgcc tgtccagcca
     1741 ctcgcctcgt agccggacac aaattcgccc aaggctggga catcgggcag taggtccagg
     1801 cgcgtcgccg cggtcaccgc aagcgcgcgt agcttgccgg ttcggatgtg ctcgatcacc
     1861 gcacctattg taggaaatcc aacctgcacc tgtccggcga g
//
LOCUS       DATLJT010000599         2516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2793671,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000599 DATLJT010000000
VERSION     DATLJT010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793671"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1065
                     /locus_tag="VM910_13915"
     CDS             280..1065
                     /locus_tag="VM910_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HVI63663.1"
                     /translation="MNDWLHNLPVVWMALLIFGLTALVTAAIYGVVTVLSVGEPARSF
                     KAVSPGLLPPLGILFALFMAFTATQVWSDNEKANSVVDREASALRTVVILAAAFPGEP
                     EARLRNLIRSYVADAVAQEWPMMAHRTATLQVIPYSLAEALQFTLAMTPSGQGQQTAQ
                     REIAVALENALDARRQRIIISQSEVNWVKWLCLSLQAVCALLAIAMVHSDNRLTSAIT
                     IGLFGVGVAASLLLILAYDRPFTGEFAVNPNPLLQVMPALGAS"
     gene            complement(1201..1902)
                     /locus_tag="VM910_13920"
     CDS             complement(1201..1902)
                     /locus_tag="VM910_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HVI63664.1"
                     /translation="MAPFLLGTVALLLLLWAAAAFSNADPKQVARLLRWIGGVAALLL
                     AAFLLFRGEAPVAIPIGAFGLGLLGWTSFWPASFTARTQKAAGQASRVRTTYLEMELD
                     HDTGRMHGRVLAGHHQGASLDALDRATLLELFREVDQDSRDLLATYLDRREPGWREAA
                     QSDAGASARAGNTGNMGEDEAYQILGLAPGATPEEIGRAHRSLIKKLHPDQGGTTYLA
                     ARINEAKDVLLRRHR"
     gene            complement(1905..>2516)
                     /locus_tag="VM910_13925"
     CDS             complement(1905..>2516)
                     /locus_tag="VM910_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI63665.1"
                     /translation="PAGRRGRLIFALDATMSRQPTWDQACGLQAEMFREAAAVGGLDI
                     QLVYYRGLAECRASSWVADPNRLRALMSGIDCRGGQTQIAKVLDHATRENDSGKVAVV
                     VFIGDAVEEALDDLCARAGELGLRNVKAFMFQEGYDAVCEQAFREIARLTGGAFCRFT
                     PGAAQELAELLRAAAAYAAGGLPALADLKAQGSGVARLIEQLK"
BASE COUNT          501 a          749 c          742 g          524 t
ORIGIN      
        1 aggatcagaa tggcggccgc agcggaatag ccgatcgtac gcattttaat tctccatctg
       61 agctgcccac ggcggggtcg gtgatgattg cacggtagtt tttctgacca ttttggtatg
      121 tgaccggtat cacgcattga cacttttgcg actactcatt ggtaaatgtt gatttttcga
      181 ggtggctagc gacgggcaac ctgatccgaa ttccgctgac ccacccaggg gagctgatcc
      241 atcatcctcg tagactaggt tgtgacgcat aggacgatca tgaacgactg gctgcataat
      301 ctccccgtgg tgtggatggc actgttgatt ttcggactca cagctttggt cacggctgca
      361 atttatggag tggttacggt gctgtcggtc ggcgagccag ctcgttcatt caaagctgtc
      421 tccccagggt tactaccgcc gctcggtatc ctgttcgctc tttttatggc gttcaccgcc
      481 acgcaagttt ggagcgacaa cgagaaggct aactcggttg tcgatcgcga ggccagcgca
      541 ctgaggaccg tcgtgattct ggccgcggcc tttccgggag aacccgaggc acgcttgcgt
      601 aatcttattc ggagctatgt cgcggatgct gtagctcaag aatggccaat gatggctcat
      661 cgcacggcaa cgcttcaggt aatcccctat tccttggccg aggcgttgca gttcaccctt
      721 gctatgacgc cgagcggtca aggccaacag acggcacagc gcgagatcgc cgttgcgctc
      781 gaaaacgcat tagatgctcg acggcaacgc atcatcatta gtcaatcgga ggtcaattgg
      841 gtcaaatggt tatgtctaag cttgcaagca gtctgcgcgt tgctcgccat tgcgatggtg
      901 catagcgaca atcggctcac ttccgcgatc acaatcggcc ttttcggagt cggagtggcc
      961 gcatctttgc tactgatcct ggcgtacgac cgacccttca ccggagagtt cgctgtgaat
     1021 cctaacccgc tattgcaggt gatgccggcg ctcggagcca gctagaagga tcaggccttt
     1081 atccgagcat cggttagatg atccgcatgt cccgttacgg cacgaagcgg cgaatatccg
     1141 ttgtaagtct cgcgcagttg ccgggccgcg gctgcggctt gaccggttgg cccggtggct
     1201 tcagcgatgc ctacgaagca gcacgtcctt tgcctcattg attcgtgctg cgagatatgt
     1261 cgtccctcct tgatcgggat gcagtttttt tatcagagag cggtgcgcgc gaccgatttc
     1321 ttcgggggta gccccgggcg caaggccaag gatctgatag gcttcgtctt cgcccatatt
     1381 accggtgttt cctgctcgcg cgctggcacc cgcatctgac tgcgccgctt cgcgccagcc
     1441 aggctctcta cgatccagat aggtcgcgag tagatcgcgg ctgtcctgat caacttcgcg
     1501 aaacaattcc aacagcgtgg ctcggtccag cgcatcaagc gaagcgcctt gatggtggcc
     1561 cgccagaacg cggccatgca ttcggccggt gtcatgatca agctccattt cgaggtaagt
     1621 cgtgcgcacc cgcgaagcct gccccgcggc tttttgtgtt cgcgccgtga aagatgccgg
     1681 ccaaaaggat gtccagccga gcagaccgag gccgaaggct ccgatcggga tggctacagg
     1741 agcttccccg cggaatagca gaaaggcggc aagcagcagc gcggcaacac cgccgatcca
     1801 acgcaatagc cgagcgactt gcttgggatc ggcattggag aacgccgcag cggcccagag
     1861 caacagcaga agggctacag tcccgagcag aaaaggcgcc atggttattt cagctgctcg
     1921 atcagtctcg caactccgct accttgcgct ttgaggtcgg caagggccgg aagtccgcca
     1981 gcggcatacg ccgcggctgc acgcaaaagc tcggcgagct cttgagcggc tcccggcgtg
     2041 aaacggcaga acgccccgcc ggtcaggcga gcgatctcgc gaaacgcctg ctcgcagacc
     2101 gcatcgtaac cttcctgaaa catgaacgcc ttgacgttac gcaggccgag ctctccggcg
     2161 cgggcgcaaa ggtcatcgag ggcttcttcc accgcatcgc cgataaacac cacgacggcg
     2221 accttaccgc tgtcgttttc ccgcgtggcg tggtccagaa ctttggcaat ctgggtctga
     2281 ccgccgcggc aatcgatgcc tgacatgagc gcccgaagcc gattcgggtc cgcaacccag
     2341 gaggaagcgc ggcactccgc aaggccacga taatagacca gctggatatc caggccgccg
     2401 acagcagccg cttcgcggaa catctcggcc tgcaacccac aggcctgatc ccatgtcggc
     2461 tgacggctca ttgtggcgtc gagggcaaag atcagtcggc cacggcgccc tgcggg
//
LOCUS       DATLJT010000600         2349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_954629,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000600 DATLJT010000000
VERSION     DATLJT010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954629"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..546)
                     /locus_tag="VM910_13930"
     tRNA            complement(477..546)
                     /locus_tag="VM910_13930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Glu,seq:ctc)
     gene            complement(588..1595)
                     /locus_tag="VM910_13935"
     CDS             complement(588..1595)
                     /locus_tag="VM910_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI63666.1"
                     /translation="MTVTIGRRELLAALGGATAAWPLAAARAAGGDAGAAYRGHHERI
                     GTEHPMTEASPVPLSRRTTSGAAAAGAALATATFSGTSSMRAQTRSKGTFVLVHPAWF
                     GGWCWKKVAPLLRERGYDVYTPTLTGLGDRAHLARPEIGLNIHIEDVANLLKSEDLAG
                     VILVGNSSGGMVITGVADQAPELIAHIVYLDAFVPQDGQSMLDIMPPDGRPPMEALVR
                     NEGQGWLLPRLAPPPWEKFVPEAWRITDEADLRWILPRLCPTPFGHFKEPVHRRNAAA
                     EKLPRTYIRCVQWPNAVFDRYAEAARQTPGWRYRELATSHIPYITHPNQLADLLIEVI
                     E"
     gene            complement(1901..>2349)
                     /locus_tag="VM910_13940"
     CDS             complement(1901..>2349)
                     /locus_tag="VM910_13940"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVI63667.1"
                     /translation="WLSGKRTLPSETLAYVRSVTGHSAEEWKRPDARTWQVSIASDTP
                     CVETTKLAAKPAPATPSAPPAPSAAWAVQLIGDRLESNALSRYAQLQKKYQAILGGRE
                     PLVVRTTMGNAAIWHRISIAADTREAAETLCSRLRAAGGSCLVQSI"
BASE COUNT          417 a          753 c          746 g          433 t
ORIGIN      
        1 cggagcgcag ttcggcaccc tgaatctgtg tgaaggcgag gccatgcgcg catagtcgca
       61 atgcatcctc ctgaatggac ggagtatcga cgacaaaacc cggtcatccg actacgccac
      121 gcgtgaacta gggcgggtca cgctcgccga tcatctcggg tcggccgggc ttggcccact
      181 cggatgaggg tcggattaaa tgagggagcc gaattttttc tgctgtggcg ggtgcgggag
      241 gggtgcccac atttgtcgag cgccggtccc gacgggtggt gcgacccatc gtaggagaac
      301 gggacgcaga ggcttcgcgc ggatcatccc agagcttaac gttgcaacgc ccgtaaggac
      361 gctgcgaacc gctgcaacag ccctcgttca cgcccgactg caaacatcta tgccgtatat
      421 ttgccgtacg gcctaggcca gccggtggtt tgttttgcct aagctattga aatatctggc
      481 gctccctagg ggactcgaac ccctgttttc gccgtgagag ggcgatccat cgcacaccgt
      541 gctggcccag tgagttgagt ctgcatggga gcatcgcccg cgacccgtca ttcaatcact
      601 tcgatcagca agtcggccag ttggtttggg tgcgtaatgt atggaatgtg ggaagtcgcc
      661 agttcgcggt accgccatcc tggcgtttgt cgggctgctt cggcatagcg gtcgaaaacc
      721 gcatttggcc actgcacaca gcgaatgtag gtgcgcggca gcttctcggc agccgcattc
      781 ctgcggtgta ccggctcctt gaaatgtcca aacggcgtcg gacagagacg aggcaatatc
      841 cagcgcaggt ctgcctcatc ggtaatgcgc caggcctccg gcacgaattt ttcccacggc
      901 ggcggtgcca atcgtggcag gagccaacct tggccctcgt tccgcaccag cgcctccatc
      961 ggaggccgcc cgtcgggtgg catgatgtcc agcatgctct ggccgtcttg cggcacgaag
     1021 gcatccaaat agacgatatg ggcgatcagc tccggcgctt gatcggcaac accggtaatg
     1081 accatcccac cagagctatt cccgaccagg atgacaccag ccagatcctc ggacttcaaa
     1141 agattggcga cgtcctcgat gtgaatgttg aggccgattt cggggcgagc gagatgcgcg
     1201 cgatcgccaa gccccgtgag cgtgggggta taaacatcgt aaccgcgctc acgtagcagc
     1261 ggggctactt tcttccaaca ccagccaccg aaccaggctg gatgaaccag cacgaaagtc
     1321 cccttgctgc gagtctgcgc tcgcatgctc gacgtcccgg aaaacgtcgc ggtcgccagc
     1381 gcggcgccag cagccgcagc gccggaagta gtccgacgtg aaagagggac aggcgaggct
     1441 tcagtcatag gatgctccgt cccaattctt tcgtgatgtc cccgatacgc cgcaccggca
     1501 tcgccgcctg ctgcgcgcgc cgccgcgagc ggccacgccg ccgttgcgcc gccgagtgcg
     1561 gccagcaatt cccgccgtcc gatggtaact gtcatgcgcc cctcccgtgc gagggtgcgt
     1621 aaatgcaacg ataccacgcc acgagcgcgc tgtcctaact gcgtgccagc cagcgcgggc
     1681 ggggcggcac gcaccggctt caatggatcg ccgcccgggc cacgctcgcg cagacgagat
     1741 tccgtttttt gcgcgcggcc tggaaggctt cacgccgctc gccgagcgga gccgctatgc
     1801 gatcgccgag agccgcactg ccgtcgtgtc aggtgctgct gcggccgcgg gcaaccgtgc
     1861 caccctcgca atgacaaggc gagtgtacgt ctggcaacgt tcagatgctc tggacgaggc
     1921 aactgccgcc cgccgcgcgc agccgcgagc acagtgtctc ggccgcttcg cgagtatccg
     1981 ccgcgatgct gatgcgatgc cagatcgccg cgttgcccat ggtcgtgcgc acgacgagcg
     2041 gctcgcggcc gccgaggatc gcctgatatt tcttttgcag ctgcgcgtat cgggagagcg
     2101 cgttgctctc caaacgatcg ccgatgagct gcaccgccca ggcggcgctt ggcgcaggcg
     2161 gtgcgctcgg ggttgcaggc gcgggttttg ccgcgagctt cgtcgtctcg acgcatggcg
     2221 tgtcgctggc aatgctgacc tgccaggtcc gcgcgtccgg ccgcttccac tcctcggcgg
     2281 agtgaccggt cacgctgcga acgtaagcga gcgtctccga tggcagcgtg cgttttccac
     2341 tgagccagt
//
LOCUS       DATLJT010000601         1568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_724583,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000601 DATLJT010000000
VERSION     DATLJT010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724583"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1568
                     /gene="dnaE2"
                     /locus_tag="VM910_13945"
     CDS             <1..>1568
                     /gene="dnaE2"
                     /locus_tag="VM910_13945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600987.1"
                     /note="DNA polymerase involved in damage-induced
                     mutagenesis and translesion synthesis. It is not the major
                     replicative DNA polymerase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVI63668.1"
                     /translation="PEEMHRLFSRYPEALARTIAIMERCRFSLDELSYQYPEERDDPA
                     LTPQQTLEELTWAGAAERYPEGLPDDVRQTLEHELRLIAKLGYAPYFLTVNSIVRFAR
                     SLDILCQGRGSAANSAVCYVLGITSIDPGRNDLLFERFVSEERQEPPDIDVDFEHERR
                     EIVMQWVFDHYGRDRAALCSTVIRYRTKGALRDVGKALGLTDDLIKTLSSQVWGWSEE
                     GIEPKHVEELNLNLRDRRLRLALDLARQLMGAPRHLSQHPGGFVLTRDRLDELVPIEP
                     AAMVDRQVIEWDKDDVAALRMMKVDVLALGMLTCMKRGLDLLAGYKGIKLDLASIPAE
                     DPRTYAMIRRADTLGTFQIESRAQMSMLPRLKPRTYYDLVVQVAIVRPGPIQGDMVHP
                     YLRRREGLEPVRYPKPELEKVLGKTLGVPLFQEQAMRVAIECAGFTPGEADRLRRSMA
                     TFKHTGGVSAFKEKLVAGMVANGYERAFAEQTFKQLEGFGSYGFPESHAASFALIAYA
                     SAWLKCWHPDVFCAA"
BASE COUNT          288 a          504 c          490 g          286 t
ORIGIN      
        1 cctgaagaaa tgcaccggct cttcagccgc tatcccgagg cgctcgcgcg cacgatcgcg
       61 atcatggagc ggtgccggtt cagccttgac gaactgtcct accagtaccc cgaggagcgc
      121 gacgatccag cgctgacgcc gcaacagacc cttgaagagc tgacttgggc aggcgcggcc
      181 gaacgctatc ccgagggact gcctgacgac gtacgccaga cccttgagca cgagctgcgg
      241 ctgattgcga agctagggta cgccccatac ttcctcacgg tgaactcgat cgtgcgcttc
      301 gcacgttcgc tcgacatcct gtgtcagggc cgcgggtcgg cagccaacag cgccgtctgt
      361 tacgtgctcg gcatcacctc gatcgacccc ggccgcaacg acctgctctt cgagcgcttc
      421 gtgtccgagg agcgccagga gccgcctgac atcgacgtgg atttcgagca cgagcggcgc
      481 gagatcgtca tgcagtgggt gttcgaccac tacggccgcg accgtgccgc tctctgctcg
      541 accgtcattc gctacaggac caagggtgcg ctgcgtgatg tcggcaaggc gctcggtctc
      601 accgacgacc tcatcaagac actgtcgagc caggtctggg gctggtcgga agaaggaatt
      661 gagccaaaac acgttgagga actgaacctc aatctccgcg atcgccgctt gcggctcgcg
      721 ctcgatcttg cgcgtcagct tatgggcgca cctcgccatc tcagccagca tcccggcggg
      781 tttgtcctga ctcgcgaccg gctcgacgaa ttggtgccga tcgagccggc tgcgatggtc
      841 gaccgccagg tcatcgaatg ggacaaggat gatgtcgccg cgctccgcat gatgaaggtg
      901 gatgtgctgg cactcggcat gttgacctgc atgaagcgcg gtctggatct cctggccggt
      961 tacaagggca tcaaactgga cctagcctcc attccggccg aggacccgcg cacttacgcg
     1021 atgatcaggc gagccgacac ccttggcacg tttcagatcg aaagccgcgc gcagatgagc
     1081 atgctgccgc gcctgaagcc gcgcacctat tacgacttgg tcgttcaggt ggcgatcgtc
     1141 cgcccgggcc caatccaggg ggatatggtt cacccgtatc tgcggcggcg cgagggcctt
     1201 gagccggtcc gctatccaaa gcccgagctg gagaaggtac tcggcaagac gctcggcgtg
     1261 cccctatttc aggagcaagc gatgagggtc gcgattgaat gtgccggctt cacgcccggc
     1321 gaagccgacc ggctccggag atcgatggcg accttcaagc acaccggcgg cgtctcggcc
     1381 ttcaaggaga agcttgtcgc cggcatggtc gcgaacggct atgagcgcgc ctttgccgag
     1441 cagaccttca agcagttgga aggcttcggc agttacggat tcccggaaag tcacgcagcc
     1501 tcttttgcgc tgatcgcgta cgcctcggcc tggctcaaat gctggcaccc cgacgtgttc
     1561 tgcgcggc
//
LOCUS       DATLJT010000602         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_266281,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000602 DATLJT010000000
VERSION     DATLJT010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266281"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1309
                     /locus_tag="VM910_13950"
     CDS             425..1309
                     /locus_tag="VM910_13950"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HVI63669.1"
                     /translation="MQGSLDDNDWKLLLHRIKEGRCTPFLGAGAAFPVLPLGRDVAEQ
                     WSTEHSYPLADKGDLPRVAQYLATNFDPMFPKERLAESFRKCAPPDFSARDEPHGVLS
                     RLPLPIYMTTNYDDLMIRALKAQGKEGLRDTCRWKAEIKPSELGRGYVPSAERPLVFH
                     LHGHLDAPRSMVLTEDDYLDFLVRITTDQRILPPRIKEAFTDTTVLFLGYGLADWNFR
                     VLFRALVEYLAKSSSRSHVSVQLLPVPQDMTAEEKQNVQKYFDKHFGKLYIRVFWGTC
                     RDFVSELSQRWEAFNGSG"
     gene            1296..>2672
                     /locus_tag="VM910_13955"
     CDS             1296..>2672
                     /locus_tag="VM910_13955"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63670.1"
                     /translation="MAAADQKPYVGPRPFGRDEKDLFFGRDREARDLCSLVVAHPIVL
                     LHADSGAGKSSLLNAGLIPLLEARGYEVLPPARVSGRIPDAIKTSEVNNIYAFNVLSS
                     WAGPSVDPRDLVKVDIASFLAKRPHRKDPADEPVLRVALLDQFEELFTFAPERWQERA
                     ALIRQLAEALEGDPLLRIVLVMRREFVGQLETHAPLLPEDVRITPFALDQLEPEAAQE
                     AIEGPLARTDKKIEPEASQRLVQELRTITVRGAGGEDVETIDPSIEPVGLQVACAKLW
                     DDLPPDVHTITAAHIETYGDVDKALKRFYEDALDAAATRTGVSKDRLRLWVDRYLITP
                     GGTRGMVYRGPESTGREEWAIPNAAVDVLDEKHLIRAEARAGGERWYELTHDRFVGPI
                     KDSNGVWKEHYERRQRRWKVLRAAGIGASVGVAAIVAVAVVVIPWYTARLTEYDRTDG
                     EIERLLTLN"
BASE COUNT          536 a          827 c          828 g          481 t
ORIGIN      
        1 ggaggaaagg cctggcgcgg ctttcaccag aatacgcggc gtcttcatgg ctgcgatgtg
       61 cgtagtggaa acgtgagatt acgcctccga gtcccgccgg caagttcagc acccgctcca
      121 tccgagatag ctggaaacga ctagagctcg gcgcgcctgg accccggcac gggctgcgct
      181 actatagttg taattctaat aaaatgcaat cactcgttgc gactgcacgc gaaactcgtg
      241 caaacccgcg aatggcggaa taaaattggc ggcgcggggc atcggccaag atcgttcccg
      301 cccgtaccgg gccggaaccg tcagtgccgt ggttcgatgc gtgagccacc tcacacagcg
      361 acgctgcccc gcagtctaaa attgtcctgt gcaacgctgc gaccagactt catcgggcac
      421 ggtcatgcag ggttcgcttg acgacaacga ctggaaactg ctccttcacc gtatcaagga
      481 gggcagatgc acgccgttcc tcggcgcagg tgctgcattt ccagtcctcc ccctcggccg
      541 cgacgtcgcc gaacaatggt ccaccgagca cagttacccg ctcgcggata agggagacct
      601 gccgcgcgtc gcgcaatacc tcgccaccaa cttcgatccg atgttcccga aagagaggct
      661 cgcggagtcg tttaggaaat gcgcacctcc ggatttcagc gcgcgggatg agccacatgg
      721 cgtgctgtca aggctgccgc tgccgatcta catgacgacc aactacgatg acttgatgat
      781 ccgcgccctc aaggcgcaag gaaaagaggg tttgcgcgac acttgccgct ggaaggccga
      841 gatcaagccg tccgaactcg gtcgcggtta cgtgccttcg gcagagcgac cgctcgtctt
      901 tcacctgcat ggtcaccttg acgcaccacg gtcgatggtg ctgaccgagg atgattactt
      961 ggacttcctc gtgcggatca ccaccgatca gagaatcctg ccgccacgga tcaaggaggc
     1021 gttcaccgat accacggtgt tattcctcgg ttacgggcta gccgactgga actttcgcgt
     1081 actgttccgc gcactcgtcg agtacctggc gaaaagcagc agccgctcgc atgtgtcggt
     1141 tcaattgttg ccagtcccgc aggacatgac tgccgaggaa aaacagaacg ttcaaaaata
     1201 cttcgataag cacttcggta agctttacat ccgggttttc tggggcacct gccgcgattt
     1261 cgtctccgag ctgagccagc gctgggaggc ctttaatggc agcggctgac cagaagccct
     1321 acgtcggacc acgtcctttc gggcgcgacg aaaaggacct cttcttcggg cgcgaccgcg
     1381 aggcgcgtga tctgtgctcc ctcgtcgtcg cccatccgat cgttctcctc cacgcggatt
     1441 ccggcgcagg caagtcttcg ctgctcaatg caggcttgat cccgctgctc gaagcgcgcg
     1501 gttacgaggt cctgccgccg gcccgcgtga gcggccggat accggatgca atcaagacga
     1561 gcgaggtcaa caacatttat gcgttcaacg tcttgtcgag ctgggccggc ccgagcgtcg
     1621 accccaggga cctggtgaag gtcgacatcg ccagcttcct cgccaagcgc ccgcatcgaa
     1681 aggacccggc agacgaaccg gtgctgcggg tcgctctcct ggatcaattc gaggaactct
     1741 ttacgtttgc gcccgagcgc tggcaggagc gcgcagcgtt gattcgccag cttgccgaag
     1801 cgctcgaagg cgatcccttg ctgcgcatcg tgcttgtcat gcgacgggag ttcgtcggtc
     1861 agctcgagac gcacgccccg ctattgccgg aggacgtgcg catcacgccg tttgcactcg
     1921 accaactgga gcccgaagcg gcgcaggagg cgatcgaggg gccactcgcg cggaccgaca
     1981 aaaagatcga gccggaggcg agtcagcggc ttgtgcaaga actgcggaca atcacggtaa
     2041 gaggggcggg tggggaggac gtcgagacaa ttgacccgtc gatcgagccg gtgggactgc
     2101 aagtcgcctg cgcgaaactc tgggacgatc ttcccccgga tgtccacacg atcacggccg
     2161 cgcacatcga gacctacggc gacgtcgata aagcgctgaa acggttctac gaagatgcgc
     2221 tcgacgctgc cgcaacgaga acgggcgtgt ccaaggaccg cctgcgtttg tgggtggacc
     2281 gctatctcat tacgcccggg ggaacgcgcg gcatggtcta tcgcgggccg gaaagcaccg
     2341 gccgtgagga atgggcgatc cctaacgccg ccgttgatgt cctggacgag aagcacctca
     2401 tacgcgccga ggcgcgcgcc ggcggcgagc gttggtacga actcacgcac gaccgctttg
     2461 tcgggccgat caaggactcc aatggagtct ggaaagaaca ctacgaacgc cggcagcggc
     2521 ggtggaaagt gctgcgcgcc gcggggatcg gtgcgagcgt cggtgtggcg gccatcgtcg
     2581 ctgtggcagt cgtggtcata ccttggtaca cggcacgtct caccgagtac gatcgcactg
     2641 acggcgagat cgagcgcctg ctcacgctca ac
//
LOCUS       DATLJT010000603         7461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2333704,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000603 DATLJT010000000
VERSION     DATLJT010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333704"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VM910_13960"
     CDS             complement(<1..845)
                     /locus_tag="VM910_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI63671.1"
                     /translation="MITGTELLEALPVAVYTTDAEGRITFYNRAAAELWGHHPELGSS
                     QWCGSWRLYWSNGRPLPHNECPMAIALKEGREVRDVEAIVERPDGTRVRFRPYPTPLR
                     DPSGQLVGAINLLMDVTERHDADMESARLAAIVVSSDDAIISKTLEGRITSWNASATR
                     IFGYDANEMIGDSILRIIPPELHGEEREILARLQRGERIEHYETVRVAKDGRRVDVSL
                     TVSPLRDRSGKVVGASKVGRDITDRKRAEKLQRVLTDELAHRVKNTLATVQAIANQSL
                     VRAKSP"
     gene            complement(1179..1457)
                     /locus_tag="VM910_13965"
     CDS             complement(1179..1457)
                     /locus_tag="VM910_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HVI63672.1"
                     /translation="MALRKFIIERDIPKVGTFEREQLRGAAAKSNEVLHQLGPDIQWV
                     ESYVADNKTFCVYLAKDEAIIRKHAEISGFPATKITEVKKMIDPTTAA"
     gene            complement(1691..2860)
                     /locus_tag="VM910_13970"
     CDS             complement(1691..2860)
                     /locus_tag="VM910_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVI63673.1"
                     /translation="MANSDSSAGLALASQRHLFEIPDDVAFLNCAYISPLPKASLIAG
                     DRGLRRKAQPWTIAAADFFTTSETVRKLFADLINANADDIAFAPAVSYGMAQAAHNIP
                     IRKTQRIVTLSEQFPSNVYPWMDLAERTGAAFVSVPRPGDDDWTAALLSFIDASTGIV
                     AVPQCHWTDGGLIDLEAIATACRSVGAALCVDATQSVGALPFDVKRVDPDFVGVASYK
                     WLLGPYSLGFLYVAPRWQRGRPIEHNWIAREDSEDFAGLVNYSHDFQPGARRFDVGER
                     SNFALMPVAEASLKLILQWTVPRISETLRLRTSAIAERARAEFGIASVPPHRRAGHYL
                     GLRFRGGVPSDLPKRLAAANVYVSVRGHAMRVTPHVWNTDEDAEKLFAVLKAELS"
     gene            3898..4053
                     /locus_tag="VM910_13975"
     CDS             3898..4053
                     /locus_tag="VM910_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HVI63674.1"
                     /translation="MLGTILLIILILILIGSLPTWPYSAGWGYYPSSGLGLVLIIVLI
                     LVLLGRI"
     gene            complement(4148..5203)
                     /locus_tag="VM910_13980"
     CDS             complement(4148..5203)
                     /locus_tag="VM910_13980"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63675.1"
                     /translation="MSLRHADCRRQKAHLAAGTRICRYMRPRYAVLAALVVCGFRFDG
                     AGPAAAQSWPTRPLTMVVPFAPGGGTDVLGRIVGRRLSEVLGQQVIIENVGGAGGMVG
                     SARVAKAAPDGYQFVLGSRADAINQTLYKNPLYNFVADLAPVVLIADQPTLLVARKDF
                     PADNLQEFIAYAKANQSRMQMASAGAGSTGHLDCMLLNAAIGINVTHVPYRGGGPAMQ
                     DLIAGRIDYFCTLSATAKQQIEGKLVKAIAILSRDRSSMLPELASTQEQGLNFEASTW
                     FGFFFPRGTPEPIIEKLHDATVAAMDTPSVQERLKEVGAVTVAPERRSPDYLQKFVAS
                     EIAKNAAPIRAAGLAID"
     gene            complement(5237..6154)
                     /locus_tag="VM910_13985"
     CDS             complement(5237..6154)
                     /locus_tag="VM910_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVI63676.1"
                     /translation="MAPRAYWKGYLKLSLVSCPIALSPAASSSERISFRQINKKTGNR
                     LRQQLVDDETREPVAAEDKGRGYEVDKGVYIEVDDDELEAIQVESRHTINIDSFVPAA
                     QIDKRFYDSPYYIAPTDSVGQEAFAVIRDAMRDKGMVGLGRIVLAKREHVIALEPCGK
                     GMLGTTLRYAYEVRKPEEYFSDIEDIKVPGEMLKLAEHILDTKAGDFDPTAFEDHYET
                     ALVELLRKKQAGFKPPKTKEHVAAPRNVINLMDALRRSVQAERAAPAQAKKGRKRIEG
                     QREMLLPIEGKQAPQRAGAKKSGARRKAG"
     gene            6636..6902
                     /locus_tag="VM910_13990"
     CDS             6636..6902
                     /locus_tag="VM910_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63677.1"
                     /translation="MRNLLLAVATGALLVAAVPASAQVYMGADPRGAGVQVGPFGFGV
                     GPRYGYDRWRGDEYYAYGADCPLVRERIVTSDGRTIIRTHRSCR"
BASE COUNT         1324 a         2337 c         2314 g         1486 t
ORIGIN      
        1 gggctcttgg cacgaaccag cgactggttt gcgatcgcct gcaccgtcgc gagcgtgttc
       61 ttgacgcgat gcgcaagctc gtcggtcagg acgcgctgca atttctcagc ccgcttgcgg
      121 tcggtgatgt cgcggccgac cttcgatgcg ccgaccacct ttcccgatcg gtcgcgcagc
      181 ggagatacgg tcagcgatac gtcgacgcgg cggccatcct tggcaacgcg cacggtctcg
      241 taatgctcga tgcgttcgcc tcgttgcagc cgcgcgagga tctccctctc ttcgccgtgc
      301 aattcgggcg gaatgatccg caggatcgag tcgccgatca tctcgttcgc gtcgtaaccg
      361 aagatgcgcg tcgcgctcgc attccacgag gtgatgcggc cttcgagggt tttgctgatg
      421 atcgcgtcgt ccgacgaaac gacgatggcc gccagtcgcg ccgattccat gtccgcgtcg
      481 tgccgctcgg tgacgtccat cagcaggttg atggcgccca cgagttgccc ggaggggtcg
      541 cgcaacggcg tcggataggg acgaaaccgc acgcgcgtgc catcgggcct ttcgacgatg
      601 gcctccacgt cccgcacctc ccgaccctcc ttgagggcga tcgccatcgg gcactcgttg
      661 tggggcagcg gccggccgtt agaccaatac aggcgccatg agccgcacca ttgactgctt
      721 ccgagctcgg gatggtggcc ccacagctcg gctgcggcgc ggttataaaa ggtaatgcgg
      781 ccctcggcgt cggtggtgta gaccgcgacc ggcagcgcct cgagcaactc agtcccggtg
      841 atcatcgcgg attccctcac gaattttgcc agctcgaccg gtcgctattg ctgccgcacc
      901 ggaccaaaac gtgcccctgt aaataaggtt ccggcccaag cggcagtggc gcgaaggcgt
      961 ccacacaaag tgtgatgtgc actgcaatag ataggattgt gcgccgcaac aacccgcgcg
     1021 ggtatcttca ggcgtctgcc cgcgccgggg gtgttgcggc tcgcgaatgc ggctctgatg
     1081 cacacaattg ctcgatggcg ccagcggcgc gccgagcgac ataacttcgg cgcttgtaac
     1141 ttcggcgttt gtaggcgggg cgacgaagtc cgcgagtttc acgcggcagt cgtcggatcg
     1201 atcatcttct tcacttcagt gatcttggtc gccggaaagc cgctgatctc ggcgtgtttg
     1261 cggatgatgg cctcgtcctt ggcgagatag acacaaaagg tcttgttgtc ggcgacgtag
     1321 ctctcgaccc actgaatgtc aggaccgagt tgatgcagca cctcgttcga ctttgccgcc
     1381 gcccctcgca gctgttcgcg ctcgaatgtg ccgaccttag gaatatcccg ttcgatgatg
     1441 aacttgcgta acgccatcgc tctcctcctt ttttggggaa tgtgttgccc tcgtaagggc
     1501 agcttgagcc ttgctgaaat tcgcgagtct gcgcggtggc cgccaagcaa gatcacgacc
     1561 aaggcgttat ctaggtcaga accgagatcg gcgcaacatt cgcccacgtc ttgccgatgt
     1621 cagagtttgg ctcgaggtcg atcgccgcgt gcggactttc cgagtgcaat aaccctgcaa
     1681 cccgagccgg tcacgacaat tctgctttca gcaccgcgaa cagcttctcg gcatcctcgt
     1741 ccgtgttcca cacatgcggg gtcacgcgca tggcatgtcc gcgcacggag acatagacgt
     1801 tggcggcggc gagacgcttc ggcagatccg acggcacgcc gccgcgaaag cgcagtccga
     1861 gatagtgacc cgcgcggcgg tggggcggca cgctggcgat gccgaattcg gcgcgcgccc
     1921 gctcggcgat ggcgctcgtg cgcagccgca gcgtctcaga gatgcgaggc accgtccatt
     1981 gcaagatgag cttgagcgag gcttcggcca ccggcatcag cgcaaagttg ctgcgctccc
     2041 cgacgtcgaa acggcgcgcg ccgggttgaa agtcatggct gtagttgacc aggccggcga
     2101 aatcctcgga gtcctcgcgc gcgatccaat tgtgctcgat gggacgaccg cgttgccagc
     2161 gcggcgcaac gtagagaaac ccgagactgt acggcccgag cagccatttg tagctggcga
     2221 cgccgacaaa atccggatcg acgcgcttca cgtcgaacgg cagcgcgccg accgactggg
     2281 tggcatctac gcagagggcg gcgccgacgc tgcgacacgc ggttgcgatc gcctcgaggt
     2341 cgatgagccc gccgtcggtc caatgacact gtggcacggc gacgatgccg gtcgatgcat
     2401 cgatgaagga gagcagcgcc gccgtccaat cgtcatcgcc ggggcgcggg accgagacaa
     2461 aggcggcccc ggttcgctcg gccagatcca tccacggata aacgttggat ggaaattgtt
     2521 ccgacagcgt cacgatgcgt tgcgtctttc ggatcggaat gttgtgcgct gcctgcgcca
     2581 tgccgtagga caccgccgga gcgaacgcga tgtcatcggc gttggcattg atgaggtccg
     2641 caaacaattt tcgcaccgtc tcgctcgtcg taaagaaatc cgccgctgca atggtccacg
     2701 gctgggcctt gcgccgcagg ccgcgatcac cggcgatcag cgaagctttc ggcaacggcg
     2761 atatgtaggc gcagttcaaa aacgcgacgt cgtccgggat ctcgaaaaga tgacgttggc
     2821 tcgcaagtgc gagtcccgcg ctcgagtctg aattggccat cgcgatccgc cgtcgttctc
     2881 acgtatcatg acgcgagccg aacgtagcgg aaaagctcgg gctgtcctaa ttccgacatc
     2941 cttcgctgca aactccgacc ccgtgggttc tgacgatccc acgtttcgtc tcgacttaag
     3001 ccccgccctg gcggggcttt ttgtgcgggg atgctctccc gcaagatcga tgattttccc
     3061 agttaagccc gtccaatccg gccgccttct cttcattgag attggtcgtg agcagccgcc
     3121 gtcctgatca cgggcaacgt gcaagcccag attcgagttc cttggatgac gggaatcccg
     3181 aacatgtggt tcggcctatt cccgagggtg cccgacgcgg cttgcgtctc gcgcacgccg
     3241 cgtcgagtta ataatgtcgc ttcgtttcgc cggatttacg tagcttcggc aacgatcaag
     3301 cttgcggcgc cggccaacgc cctgcggtca agcttttgcc atccagtctg cccaagccac
     3361 tgaccggcct cctcatcgct gtatgcttgg ccttcgcctg caatgaccag gaaactctcc
     3421 cgacataaga gcggcggcga ccggctgagt acgcgtggga tcggtccata gatccgcgag
     3481 cagaagacgg gcgcctggcc ggacgtacgc tcgcattttc cgcataagct cgatgttgtg
     3541 tgcggtcgag aacacatgga cggtgttcgc gaccatcagg gcatcgtgac cttcaggcag
     3601 agggtcttta aaaacgtctc cctccacgac ctcgatgcgc gtgccttagg gctcgcgggc
     3661 cagctctctt ggcttgcggc aacaccgctg acccgcgcca acgggaatga gcgtcgagcc
     3721 gggaacatat ccgcgctaaa agcgttctct cgctgtggaa tcctaaccgg aggaggaagc
     3781 aacgctgctg cttggcgttt cggttattcc gtcggggagg cggatcattc acatcacgct
     3841 gaagaagggt gtgagaccgc agagaaaatc cggtaaggcg aaactagaga gatgaccgtg
     3901 ctcggaacca ttctgctgat tatcttgatc ctgattctca tcggctcgct gccgacctgg
     3961 ccctatagcg ccggctgggg ctactatccg tccagcggcc tcgggctggt cctcatcatc
     4021 gttctcatcc tcgtcctctt gggcagaatc taaggttctt ccgacgagtc ctgggcatcg
     4081 gctttttctg ggccccacga aggcggcgcg gagtaactcg cctgctttgg tcagcggcca
     4141 agcggcttca atcgattgcg aggccggcgg ccctgatcgg cgcggcgttt ttcgcgatct
     4201 cgctcgcgac gaatttttgc aaatagtcgg gtgagcggcg ctccggcgcg acggtcaccg
     4261 cccccacctc cttgagtcgc tcctgcaccg aaggcgtgtc catcgccgcg acggtggcgt
     4321 catggagctt ctcgatgatc ggctccggcg ttcccctggg gaaaaagaag ccgaaccagg
     4381 tcgaggcctc gaaattgagc ccctgctcct gcgtgctggc gagctccggc agcatcgacg
     4441 agcgatcgcg cgagaggatc gcaatggcct tgaccagttt gccttcgatc tgctgcttgg
     4501 cggtcgcgct tagcgtgcag aagtagtcga tgcggccggc gatcagatcc tgcatcgccg
     4561 ggcctccccc gcggtatgga acgtgggtga cgttgatccc gatcgccgcg ttgagcagca
     4621 tgcagtcgag atggccggtg gaccccgctc ccgccgaagc catttgcatc ctcgactggt
     4681 tcgccttcgc ataggcgatg aactcttgca gattgtcggc ggggaagtcc ttgcgcgcga
     4741 ccaagagcgt aggctgatcc gcgatcagca ctaccggcgc gagatcggca acgaaattgt
     4801 agagtggatt tttgtaaagc gtctggttga tggcgtcggc cctgctgccg agcacgaact
     4861 gatagccgtc cggagccgcc ttcgcgacgc gcgccgaacc caccattccg ccggcgccgc
     4921 cgacgttctc gatgatcacc tgctggccca gaacttcgga gagacgccga ccgacgatgc
     4981 gcccgagcac gtcggtgccc ccgccgggcg cgaacgggac caccatggtc agcgggcgcg
     5041 tcggccagct ctgtgcggcg gccgggccgg cgccgtcgaa gcgaaaaccg cacacgacca
     5101 aggcggccag cacggcatat ctcggacgca tgtatcggca aattctggtt ccggctgcga
     5161 gatgagcctt ctgccgccgg caatcggcat ggcgcaatga catcggcgca atccgccgca
     5221 ccatgagcgc cgcaggtcag cccgctttgc ggcgcgcgcc cgatttcttg gcgcccgctc
     5281 gctgcggcgc ctgcttgccc tcgatcggca gcagcatttc ccgctgccct tcgatccgct
     5341 tgcggccctt cttcgcctgc gccggcgccg cgcgctcggc ttgcacgctg cgacgtagcg
     5401 catccatgag attgatgacg ttgcgaggcg cggcgacgtg ctctttggtt ttcggcggct
     5461 tgaagccggc ctgcttcttg cgcagcagct ccaccaacgc cgtctcgtag tggtcctcga
     5521 acgcggtcgg gtcgaagtca ccggccttcg tatccagaat gtgctcggcg agcttcaaca
     5581 tctcacccgg cactttgatg tcctcgatgt cgctgaaata ttcctccggc ttgcgcactt
     5641 cgtaggcgta gcgcagcgtc gtccccagca tccccttgcc acacggctcc aaggcgatga
     5701 cgtgctcgcg cttggcgagc acgatgcggc cgagcccgac catgcccttg tcgcgcatgg
     5761 cgtcgcggat gaccgcgaac gcttcctgcc caacgctgtc ggtcggcgca atgtaatagg
     5821 ggctgtcgta gaagcgcttg tcgatctgcg ccgcggggac gaagctatcg atgttgatgg
     5881 tgtgcctgct ctccacctgg atggcctcga gctcgtcatc gtcgacctcg atgtagacgc
     5941 ccttgtcgac ttcgtagccc ctgcctttgt cctcggccgc gacgggctcg cgcgtctcgt
     6001 catcgaccaa ttgctggcgt aagcggttgc cggtcttctt gttgatttgc cgaaacgaga
     6061 tgcgctccga cgacgacgcg gcgggactga gcgcgatggg gcacgagacg agcgaaagct
     6121 tgagatagcc cttccaatat gcacgcggcg ccacggcaat actcctacaa actgcgactg
     6181 cgcttaagtt aacaattttt ccgcgcctct tggagtcctg cttttgttct cgcatccccg
     6241 atatagcgtg cgtgcggtgc gcgcgggcta gcaattgagg cggcgaaagc gcggcgcttg
     6301 cgccagctat tgacgcggtt aaaaaaatcg taacgcggct ggccgccgaa gctggtgtgc
     6361 tcaccggtcc aacgccgttc ggcaagacct ttcatcgatc gggattgcgc ggcggtcgcg
     6421 cagcgcatgc gctggtgatt gagcgcatcg cagcatcgcc acggcatcgt ggttccctca
     6481 tgtggaacgc ggcgtggcgg atcccggcga gcagcgcggc atcgatggca agcggtacaa
     6541 aacgccgggt ttccttgggt tagaagtttt tctcaacgcg caacgccagc gcgcctgatg
     6601 cgttggggcg ggagggtaat aagaaggagg aagtcatgag gaacctgtta ttggccgtgg
     6661 caaccggcgc cttgctcgtt gcggcggtcc cggcctcggc tcaggtctat atgggcgcgg
     6721 accccagagg ggctggcgtg caggtcggcc cgttcggctt cggcgtcggg ccgcggtacg
     6781 ggtacgatcg ttggcgcggt gatgaatact atgcctatgg cgccgactgt cccctcgtca
     6841 gggagcgcat cgtaacgtcg gacggtcgga cgatcatcag gacgcaccgc tcctgccgct
     6901 gacgctagtc aagcggaata gccagcaggc aaaaacgagt gatcccggaa aacctccggg
     6961 accacttgtt gcgtgaaagc aaaacgcgcg gtgatcgccg cgcgttttta ccttctgcat
     7021 cgaagcgacc gcgttccgcg cgctttcgca catcttctta gcaagccctt cgcagagatg
     7081 acgagatcgg ttcgtgcgct cgtctgcgcg cgcgatagtc gctcactgct aagcagacga
     7141 tctttcgccg gggtaaggga cgacgttcaa actggccgac caaagacagt ccgaccagct
     7201 cttgcgcttg cggacttcgg ctcgaagcca ggcgcgatag tgatcaacgc acgtctgcga
     7261 acagaagctc ttgaacgctc gacgatgccg taccagcccg aacttgccgt ggcagtaagc
     7321 gcaatgattt ttcatagctt tctcccccga gcgccacccc ggttgaaccg accggctgag
     7381 gatcacgccc tcagtgcact gcggcatcga gctattgcaa tgcacaatta gtattacatc
     7441 tatcaattga gaattcgttt g
//
LOCUS       DATLJT010000604         8351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2793940,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000604 DATLJT010000000
VERSION     DATLJT010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793940"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VM910_13995"
     CDS             complement(<1..1024)
                     /locus_tag="VM910_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI63678.1"
                     /translation="MSGKLSLGPSGHVDDFARRNLPPLHQWPDLLLDRPEFQYPEYLN
                     VAVELTDRIVEKGFGDRIALIGNGRQRTYKELADWSNRLAHALVENYGVKPGNRVLIR
                     SGNNPALVAAWLAATKAGAVVVNTMPMLRAGELGKIVDKAEIALALTDSRIADELVAC
                     AKTSRFLKQVVNFDGTSNHDAELDRVALNKPVRFDAVKTGRDDVALLGFTSGTTGEPK
                     ATMHFHRDLLIVADGYAREVLKVTPDDVFVGSPPLAFTFGLGGLAIFPLRFGATATLL
                     ENAAPGEMVKIIETYKATVCFTSPTAYRAMMGAMDKGADLSSLRLAVSAGETLPAPVF
                     ESWTRKT"
     gene            complement(1146..1961)
                     /locus_tag="VM910_14000"
     CDS             complement(1146..1961)
                     /locus_tag="VM910_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI63679.1"
                     /translation="MAEIDYEVEYNNRARVPESPALMAGWARDAASYRECHPPRVIRY
                     GPGGRHTVDVFAASDQGPIVVFIHGGYWQALDGSSFSHCARGLNEHGVDVAVPTYDLC
                     PNVTVDVIIQEMRAATRELAKLGRPLVMSGHSAGGHLAACMLATDWPAFDAFLPENVV
                     FAAYAISGLFDLEPLVETSLNRALRLDRPSARAVSPLFWKPPGHGSLDAVVGGNESAE
                     FHRQSRTMVERWGAAGVAARFGTLADANHFTAIAPLADPDSAMVRRLKELALR"
     gene            complement(1967..2461)
                     /locus_tag="VM910_14005"
     CDS             complement(1967..2461)
                     /locus_tag="VM910_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI63680.1"
                     /translation="MILDSETKAVELPEDHGNELRLWLRLLTCSTLIEGEVRSRLRER
                     FDVTLPRFDLMAQLDKAPDGMTLSDVSKRMMVSNGNVTGLVERLVESGHLDRRTSESD
                     RRVQVIRLTKIGRAEFRKMAAEHEIWIADIFSDLTPKDVRELMRLLAKTKGSAQKSAS
                     RRSP"
     gene            2757..3932
                     /locus_tag="VM910_14010"
     CDS             2757..3932
                     /locus_tag="VM910_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63681.1"
                     /translation="MTVKCKLVGLVVLLSLVRPAFAEDKIKIGVIVTLSGPAAALGQQ
                     VRDGFTLGVKDLGGKMAGKEVEVVVVDDELKPDAAVTKVKGLLERDKVDFVVGPIFSN
                     ILQAIHKPVVDSKTFLISPNAGPSSYAGKECSPFFYVTSYQNDQVHEILGKVAQDRGY
                     KRIYLLVPNYQAGRDSAAGFKLDYKGEIVEESYMPLGTLDFQPELSKIASLKPDAVFT
                     FMPGGMGVNLVKQYRQAGLADSVPVLSAFTVDESTLPAQQDAAVGMFGGADWAPDLNN
                     PQSKKFVASYEAAYHIVPGTYAMQAYDAAMLIDSAVKAVKGDLGNKDAVAAVLKKADF
                     ASLRGNFKFNTNGYPIQDFYLTKVAKRPDGKFQTEIVQKVFENYGDRYAKECAPGNN"
     gene            3955..4407
                     /locus_tag="VM910_14015"
     CDS             3955..4407
                     /locus_tag="VM910_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI63682.1"
                     /translation="MKSEVAGITRANEGIQGITWNILGQTYVPKNRTENCFSWHATFP
                     PGTFVPPHIHPDQDEYLYILEGRLDFMLDGADEFATAGDTVRLPMGKPHGIFNKSQQN
                     AKTLFWVSPTRRLYDLFWAIHNMKQQNPDEVVKLAAEHNIHFLPPPPG"
     gene            complement(4423..5667)
                     /locus_tag="VM910_14020"
     CDS             complement(4423..5667)
                     /locus_tag="VM910_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HVI63683.1"
                     /translation="MKAIIVGGGIGGLTTALMLRARGIDCEIYEQSDSIRELGVGINT
                     LPHAIRELAGIGLLDRLDDAAVRTYELFYLTRHGQQVWHEKRGLDAGHDVPQFSIHRG
                     RLQGVIHQAVIERLGSDAVRTGCRLGSFTQDEGGVTAYFFDRGGAHTYTARGDILIGA
                     DGIHSRVRETLFPEEGPPCWNGLMLWRGAHDWPVFLTGRSMIIAGGLNAKAVVYPIAE
                     GSSPASRLTNWAVLVRVGDGSAPPARREDWSRLGKPEELMPHVAGFSIPQVDFAGLVR
                     ATPEFWEYPCCDRDPLSCWSSGRVTLLGDAAHPMYPVGSNGASQAILDARCLADALAR
                     SEHPRQALLAYERQRLPMTADIVASNRRGGPEGVIDAVEQLAPQGFADIDTILSYEAR
                     EAIVRGYAAKAGFAARVVARQS"
     gene            complement(5721..8072)
                     /locus_tag="VM910_14025"
     CDS             complement(5721..8072)
                     /locus_tag="VM910_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HVI63684.1"
                     /translation="MKIAIIGGGPAGLYASILLAKQKPKAEITVYERNRADDTFGFGV
                     VFSDATLDNFEKYDPQSYRRITQEFAYWDDIAVHFRGTVHRVGGNGFCGCSRRNLLLI
                     LQERARELGVNLRFETEIDDESRLIDADLVILADGINSRFRERYIDHFEPEVDIRSNY
                     FAWMGSSRPLDAFTFIFQETEWGPFIAHAYQYEAGRSTWIFETDPETFENAGLKGLNE
                     RQSADRMAEIFGWFLGGHQLLTNRSMWRNFPMIRSKRWVKNNMVLLGDAKATAHFSIG
                     SGTKLAMEDAIALAEAMERAPTIAAALQLYEQGRREEVEKTQHAADVSLVWFEHVDRF
                     WDFDPVQFAFGVMTRSKAITYDNLKLRAPDFVAEVDKAFAKQVRAKGFEIDVARPLAP
                     MFQPMRLREMQIANRAVVSPMCMYSAKEGVPTDFHLVHYGSRAIGGAGLIFTEMTCVG
                     RDARITPGCAGLWNDEQEATWKRIVDFVHANSAAKFALQLGHAGRKGATKLMWDGMDR
                     PLEEGGWDVISASPIPYFPDSQVPREMDRAAMDQVRAAFVAATERGERCGFDMLELHC
                     AHGYLLASFISPLTNKRSDQYGGSLTNRLRFPLEVFEATRAAWPKHKPMSVRISATDW
                     AEGGITGDDAVLVARAFAEAGVDLVDVSTGQTVRDGQPVYGRMFQTPFSDQVRNEARV
                     ATMCVGNITTSDQVNTILAAGRADLVALGRPHLVDSAFTLKAAAWYGAEGVFCPPQYL
                     PGKEQIFRNSVRDRQDLEELKIKAKPKTRAELKGEATKPLAAE"
     gene            complement(8159..>8351)
                     /locus_tag="VM910_14030"
     CDS             complement(8159..>8351)
                     /locus_tag="VM910_14030"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088822.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVI63685.1"
                     /translation="QVAEGPTFANTMTKRMLSMEWAMSVEEAIEAEAVAQALCMTTAD
                     FERAFVAFSNKVKPAFQGN"
BASE COUNT         1696 a         2627 c         2456 g         1572 t
ORIGIN      
        1 cggtcttgcg ggtccagctt tcgaagaccg gcgccggcaa ggtttcgccg gcggagaccg
       61 caaggcgaag cgacgacaga tctgcgccct tgtccattgc gcccatcatc gcccgatacg
      121 cggtcggtga ggtaaagcag accgtggcct tgtacgtctc gatgatcttg accatctcgc
      181 ctggagctgc gttttccagg agcgtcgccg tggcgccgaa ccgcagggga aagatcgcca
      241 atcccccaag tccaaacgta aacgcaagcg gtggcgatcc cacaaagacg tcgtccggcg
      301 tgaccttcag cacttccctg gcataaccgt ccgccacgat cagaagatcg cgatggaaat
      361 gcatggtcgc ctttggctcg ccggttgtgc ccgaggtgaa tccgagcaag gcgacgtcat
      421 cgcggccggt cttgaccgca tcgaaccgaa ccggcttgtt cagcgcgacc cggtcgagtt
      481 cggcatcatg gttcgaggtg ccgtcaaaat tcacgacctg cttcagaaag cggctggtct
      541 ttgcgcaggc aaccagctcg tcggcgatgc ggctgtcggt cagcgccagc gcaatctcgg
      601 ccttatccac gatcttgccc agttcaccgg cgcgaagcat cggcatcgtg ttgaccacga
      661 cggcacccgc cttggtcgct gcgagccacg cggcgaccag agcaggattg ttgcccgaac
      721 ggatcaggac gcggttgccg ggtttgaccc cataattctc caccagcgca tgtgcaagcc
      781 ggttcgacca gtcggccagc tccttgtagg tgcgctggcg accattgccg atcagcgcga
      841 tgcggtcgcc gaaccccttc tcgacgatgc ggtcggtcaa ctcgaccgcg acgttgaggt
      901 attcgggata ttggaattcc ggacgatcga gcagcaaatc cggccattgg tgcagaggcg
      961 gaagatttcg gcgcgcaaaa tcatcgacat gacccgaggg gccaaggctc agtttacccg
     1021 acatatccat cgccctttca gttcgaggac gcccggcagc gtttcattcc taaacatttt
     1081 atacttaaaa taatttgacg caagggccca aatccatgca gtccagccgt ctcggcggca
     1141 tcagctcagc gcagcgccag ttccttcagc cggcgcacca ttgccgagtc cgggtcggct
     1201 agcggcgcga tggcggtgaa gtgattggca tcagccagcg tgccaaaccg cgccgcaacg
     1261 cccgcagcgc cccacctttc caccatggtc cggctctggc ggtgaaattc ggcactctcg
     1321 ttgccgccga ccaccgcatc gaggctgccg tggcccggcg gtttccaaaa caaggggcta
     1381 acggccctgg cacttggccg atcaagccgc aatgccctat tgagcgaagt ttcaaccaac
     1441 ggctcgagat cgaacagacc tgaaatggca taggccgcga acacgacgtt ttcgggcaga
     1501 aacgcatcga acgcaggcca atccgtcgcc agcatgcagg ccgcaagatg cccgcccgcg
     1561 gagtgtccgc tcatcacgag cggtcgtccg agcttcgcca gctctcgcgt cgcagcgcgc
     1621 atctcctgga tgatgacgtc gaccgtgaca tttggacaaa gatcataagt cggaacggca
     1681 acgtcgacgc catgttcgtt gaggccgcgc gcgcagtggc tgaacgaaga gccgtccagc
     1741 gcctgccaat atccgccgtg gatgaagaca acgatcggtc cttgatcgct agcggcgaag
     1801 acgtcgaccg tatgccggcc tccagggccg tagcgaataa cgcgtggcgg gtggcattcg
     1861 cggtagctcg cggcatcccg agcccagccc gccatcaacg cggggctctc cggaacccgt
     1921 gcccggttgt tgtattcgac ctcgtagtcg atctcagcca cgaggctcag ggcgatcgcc
     1981 gactggcgga tttttgcgcc gaacccttgg ttttggccaa aaggcgcatc agctcgcgga
     2041 catccttagg cgtcaaatcc gaaaagatgt cggcgatcca gatttcgtgc tcggccgcca
     2101 ttttgcgaaa ctcggcgcgc ccgattttgg tcaagcgaat gacctgcacg cggcggtcgg
     2161 actccgacgt gcggcggtcg agatgaccgg attcgacgag gcgttcgacg agcccggtga
     2221 cattgccgtt tgacaccatc atgcgcttgg aaacgtccga cagggtcatg ccgtcgggcg
     2281 ccttgtcgag ctgggccatc aggtcgaacc gcggcaaggt aacgtcgaag cgttcgcgca
     2341 accggctgcg cacctcaccc tctatcaggg tcgaacaggt cagcaggcgc agccacaatc
     2401 tcagttcgtt gccgtgatct tccgggagct cgacggcctt tgtttcagaa tcgagaatca
     2461 tactgcaatc atatcgcgtg agaggtctcg cggcatcaca gcctccgccg caatggactg
     2521 atcttttgag cttcaaataa tttagcgtgt gccgcaagtc aaaactcggc atccccgtcg
     2581 aatttgttta ggcttcaagc aaatggcgcg cggcttgcat gctgcttggt cggctggcct
     2641 tgttcgcccc tgctttgctt gcggcaacgg gcttcgtcag aataagcttg aacagggaaa
     2701 aacgcatttg gcgctacctt tggccggcgc cggcactcgc gatcggggaa gtagtcatga
     2761 cggtaaagtg caagttggta ggtcttgtgg tcctgctcag cctcgtccgg cctgccttcg
     2821 cggaggacaa gatcaagatc ggggtgatcg tgaccttgtc cggaccggcc gccgccctgg
     2881 gccagcaggt gcgcgacggt tttacgctgg gggtcaagga tctcggcggc aaaatggccg
     2941 gcaaggaggt cgaggtcgtc gtggtcgatg atgaactcaa gcccgacgcc gccgtgacca
     3001 aggtgaaggg gttgctcgag cgcgacaagg tcgatttcgt cgttggcccg atcttttcca
     3061 acattctgca ggcgatccac aagccggtcg tcgacagcaa gaccttcctg atcagcccca
     3121 acgccggtcc ctcgagctat gccgggaagg agtgcagccc attcttctac gtgacctcgt
     3181 accagaacga tcaggtgcac gagatcctcg gcaaggtggc gcaagaccgc ggctacaagc
     3241 gcatttacct tctggttccg aattatcagg cgggacggga ttcggccgca ggcttcaagc
     3301 tcgattacaa gggcgaaatc gtcgaagagt cctatatgcc gctgggcacg ctcgatttcc
     3361 aacccgaact gtccaagatc gcgtcgctca agccggatgc cgtcttcacc ttcatgccgg
     3421 gcggcatggg cgtgaatctg gtcaagcagt accggcaggc cggccttgcc gacagcgttc
     3481 ccgtgttgtc ggccttcacg gtcgatgaat cgacgttgcc tgcacagcaa gatgcagcag
     3541 tcggcatgtt cggcggtgcg gactgggctc ccgatctcaa caatccgcag agcaagaaat
     3601 tcgtcgcttc gtacgaagcc gcctaccata ttgtccctgg cacctatgcg atgcaggcct
     3661 atgacgcggc catgttgatc gacagcgcgg tcaaggcggt caagggcgat ctcggcaaca
     3721 aggatgcggt cgcagccgtc ttgaaaaagg ccgacttcgc ctcgctgcgc ggcaacttca
     3781 agttcaatac caacggctat ccgatccagg acttctatct gaccaaggtt gcaaaacgtc
     3841 cggacggcaa attccagacc gagatcgtgc agaaagtatt cgagaactat ggcgaccgct
     3901 acgccaagga gtgcgcgccg ggcaacaact gacagacagg gaaggaaacc atctatgaag
     3961 agcgaagtcg ccggtatcac ccgcgccaat gaaggcattc agggcatcac gtggaacatt
     4021 ctcggccaga catacgtgcc gaaaaaccgc accgaaaact gcttctcctg gcacgcgacg
     4081 tttccgcccg gcaccttcgt gccaccccac atccatcccg accaggacga gtatctctat
     4141 attctcgaag gcaggctcga cttcatgctc gatggcgccg acgagtttgc gaccgctggc
     4201 gatacggtgc gtctgccgat gggcaagccg cacgggatct tcaacaaatc acaacagaac
     4261 gccaaaacgc tgttctgggt ttccccgacc cgccggctct atgatctgtt ctgggccatc
     4321 cacaacatga aacagcaaaa ccccgatgag gtggtcaagc tcgccgccga gcacaatatc
     4381 catttcctgc cgccaccgcc cggataagcg acagcaccgt tctcaggatt ggcgcgcgac
     4441 gacgcgtgcc gcgaagccgg ctttggccgc atagccccgc acgatcgcct cgcgcgcttc
     4501 gtagcttaaa atagtgtcga tatcggcaaa tccttgcgga gcgagctgct cgaccgcatc
     4561 aatcacacct tcgggaccgc cgcggcggtt cgatgcgacg atgtcggcgg tcatcggcaa
     4621 tcgctggcgc tcataggcga gcagcgcctg ccgcggatgc tccgaacgcg ccagcgcgtc
     4681 cgcaaggcag cgtgcatcga ggatcgcttg cgaggcgcca ttcgatccta ccgggtacat
     4741 tggatgcgcg gcgtcgccga gcagcgtgac gcggccgctc gaccaacagg acaagggatc
     4801 gcggtcgcag cagggatatt cccaaaactc cggcgtggcg cgcacgagcc ctgcaaagtc
     4861 gacttgcggg atcgagaagc ccgcgacgtg gggcatcagt tcttccggct tgccgagcct
     4921 tgaccaatct tcgcgccgcg ccggcggcgc gctgccatcg ccgacccgca cgagcaccgc
     4981 ccaattggtg agtcggcttg cggggctcga gccttccgca atcggataga ccaccgcctt
     5041 ggcattcagt ccacctgcga tgatcatcga tcgaccggtg agaaagaccg gccagtcatg
     5101 agcgccgcgc caaagcatca agccattcca gcatggcgga ccttcctccg gaaacagcgt
     5161 ttcccggacc ctggaatgga tgccgtccgc accaatcagg atatcgccac gcgcggtata
     5221 tgtatgcgcg ccgccgcggt cgaagaaata ggcagtaacg ccaccctcgt cctgggtgaa
     5281 cgaaccaagc cggcagccgg tgcggaccgc gtcgctgcca agccgttcga tcacggcctg
     5341 atgaataacg ccctgcaggc ggccgcgatg gatggagaat tgcggtacgt cgtggccggc
     5401 atcaagcccg cgcttctcgt gccagacctg ctgtccgtgg cgcgtcaaat agaacagctc
     5461 ataagtgcgg accgcggcat cgtcgagccg gtccaacaga ccgatgccgg caagctcacg
     5521 gatcgcgtgc ggcagcgtgt tgatgccaac gccaagttcg cggatggaat cggactgttc
     5581 gtagatttcg caatcgatgc cacgggcccg caacatcagt gccgtggtga ggccgccgat
     5641 gccgccaccg acgatgatcg ccttcatggg ttaaactccg taacccgacg cctgactgcc
     5701 gatcagggtc gcacgtcgtc tcactctgcc gcaagcggct ttgtcgcctc ccccttgagc
     5761 tccgcccggg tcttcggctt agccttaatt ttaagctcct ccagatcctg ccggtcgcgc
     5821 acgctgttgc ggaagatctg ctccttgccc ggcagatatt gcggagggca aaataccccc
     5881 tccgcaccgt accaggcagc cgccttcagc gtgaaagcgg aatcgaccag atgcggtcgg
     5941 ccgagcgcaa ccaggtcggc gcggccggcg gccaggatag tgttcacctg gtcggacgtc
     6001 gtgatgttgc cgacgcacat ggtcgcaacg cgcgcttcgt tgcgaacctg atcggagaaa
     6061 ggcgtctgga acatgcggcc atagaccggc tgcccatcgc gcacggtctg gccggtcgaa
     6121 acgtcgacca gatccacgcc cgcttccgca aacgcccgcg caaccaaaac ggcgtcatcg
     6181 ccggtgatgc cgccttcggc ccagtcggtc gccgaaatgc gcaccgacat cggcttgtgc
     6241 ttgggccatg ccgcccgcgt cgcttcaaac acttccagcg gaaaccgcaa gcggtttgtg
     6301 agtgagccgc catattgatc actgcgttta ttggtgagcg gcgaaatgaa gctcgcaagc
     6361 aggtaaccgt gggcgcagtg cagttcgagc atgtcgaagc cacagcgctc gccgcgttcg
     6421 gttgcggcga cgaatgccgc ccttacctgg tccatcgccg cgcggtccat ctctcgcggc
     6481 acctgactgt ccgggaaata gggaattggc gacgccgata tgacgtccca accgccctcc
     6541 tccagcgggc gatccatacc gtcccacatc agcttggtcg cacccttgcg gccggcgtgc
     6601 ccaagctgaa gcgcaaattt cgccgcagaa ttggcgtgga caaaatcgac gatacgcttc
     6661 cacgtagctt cctgctcgtc attccacagc cccgcgcagc cgggcgtgat ccgagcatcg
     6721 cgaccaacgc aagtcatttc ggtgaaaatc aagccggcgc caccaatggc gcgcgatccg
     6781 taatgcacaa ggtgaaaatc cgtcggcacg ccctccttcg ccgaatacat gcacatcggc
     6841 gataccaccg cgcgattagc gatctgcatt tcgcgcagac gcatcggctg aaacatcggc
     6901 gccaatggcc ttgccacgtc gatttcaaat cccttcgcac gtacctgttt tgcaaaggct
     6961 ttatcgactt cggcgacgaa atcgggggcc cgaagcttca gattgtcata ggtgatcgcc
     7021 ttcgagcgcg tcatcacgcc gaaggcgaac tgaacgggat cgaagtccca gaagcggtcc
     7081 acatgttcga accacacaag ggaaacgtcc gcggcatgct gggttttttc gacctcctcc
     7141 cgccgtccct gctcgtaaag ctgcaaagcg gcagcaatcg tcggcgcacg ctccatggct
     7201 tccgcaagcg cgatggcgtc ttccattgcc agtttggtgc ccgatccgat cgagaaatgg
     7261 gcggtggcct tcgcgtcacc caaaagcacc atgttgttct tgacccagcg cttgctgcgg
     7321 atcatcggga aattgcgcca catcgagcgg ttggtcagaa gctggtgtcc cccgaggaac
     7381 cagccgaaga tctccgccat cctgtcggcc gattgtcttt cgttcaggcc tttcaggccc
     7441 gcgttctcaa aggtctcggg atcggtttcg aagatccagg tcgaacggcc ggcctcgtat
     7501 tgataggcat gcgcgatgaa cgggccccac tccgtctcct gaaagatgaa ggtaaaagcg
     7561 tcgagcggcc gggacgagcc catccaggcg aagtagttgg agcggatgtc gacctcaggc
     7621 tcgaaatgat cgatatacct ttcgcggaag cggctgttga tgccgtcggc aagaataacc
     7681 agatcggcgt cgatgagacg ggattcgtcg tcgatttcgg tttcgaagcg caggttgacg
     7741 ccaagctcgc gcgcgcgctc ctgcaggatc agaagcaagt tgcgccgtga gcagccgcaa
     7801 aatccgttgc cgccgacgcg atgcacagtg ccgcggaaat gcacggcgat atcgtcccaa
     7861 taggcaaact cctgggtgat gcggcggtag ctctgcggat cgtatttttc gaaattgtcg
     7921 agcgtcgcat cggagaacac cacgccgaag ccgaaggtat cgtcggcgcg attgcgttca
     7981 tagaccgtga tttcggcctt gggcttctgc tttgcgagca ggatcgaggc gtagagaccg
     8041 gccggtccgc caccgatgat cgcgatcttc atgagcgcct ccgacgacca ggcaacggaa
     8101 ttattttaaa cctaaaataa ttttgcgcaa gtccctgctg cggccttttg gaaagcgctc
     8161 aattcccctg aaaagccggc ttcaccttgt tggagaaggc gacgaaggct cgctcgaaat
     8221 cggccgtcgt catgcaaagc gcctgcgcca ccgcctcggc ctcgatcgcc tcctccaccg
     8281 acatcgccca ctccatcgaa agcatccgct tggtcatggt gttggcgaaa gtcgggccct
     8341 cggccacctg c
//
LOCUS       DATLJT010000605         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3482894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000605 DATLJT010000000
VERSION     DATLJT010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VM910_14035"
     CDS             <1..633
                     /locus_tag="VM910_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVI63686.1"
                     /translation="FLASDLSMAPMRHYLSELGYDTHAWRMGRNTGGVSRMRSALLDR
                     LRAVFADTGRKVSLVGWSLGGVYARDLALQAPDMVRYVITLASPFASDVRATNATRLY
                     ESVSGETVDLTSELSKSIGGDLPVPTSAIYSRADGVVNWRTSRLRASATAENIEVYFA
                     SHVGLGVNPAALWAVADRLAQAEGQFHQFNRLGPFAIAYAPPEHAQSARL"
     gene            676..2223
                     /locus_tag="VM910_14040"
     CDS             676..2223
                     /locus_tag="VM910_14040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HVI63687.1"
                     /translation="MGDGKKLSSLDASFLYLETPEMPMHVGSMAIFRLPDDYKGDFFE
                     DFKAMIASRLHVAPILKARLEKAPLDIDHPSWVEDDQFDIDRHIFRASLPAPRDRATL
                     ERIVGWMHAKLLNRARPLWEFYVFEGMKDNEIGLYSKMHHACIDGGAGAALTNMIYDI
                     SPIPREVDPPTAKAKIGQEPRDIAANLLDSYQQLWSQPLDAAAARSIELPRSGKSDLG
                     SILFDNAMYQIESAVRFAGNIPTMLKSVSDVIGKISDPKSRESLATMMSPPTILNKSI
                     SSERSFAGVSISLSRAKALAKQSGGKLNDVVLALASGVVRRYLIKQGALPNKSMTAAV
                     PISLREEGNAEANNQVFGMICSIATNVEDPKARLETIIAQSTKSKEMSHPLRALMPQV
                     SNISMLGAPIVVQILALLYSRSSLSDVLPPAANITVSNVPGPRQTLYAAGAELLHIFP
                     VSISTHGIALNITVQSYRDQLDFGFIAGANIIPHVQELCDMLPEEFSALEAAYAPPAS
                     DIKGTAE"
     gene            2235..>3118
                     /locus_tag="VM910_14045"
     CDS             2235..>3118
                     /locus_tag="VM910_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI63688.1"
                     /translation="MIEMPPLQFAQTNGIRMGYYDAGPKSDKPPLVLCHGWPELAFSW
                     RYQIKALSEAGIRVIAPDQRGYGATDRPEAVEAYDLEHLTLDLVGLLDHLGIDKAVFV
                     GHDWGGFVVWRMALSRPSRVAGVVGVNTPHTDRAPIDPIELFRKRFGEQMYIVQFQDP
                     AREPDRIFDGRVEETFDAFMRKPLQRSDAGPSEPPAAGVGASPKLNLAFPQMIAAYDA
                     RLDPRTPILSPEEKQVFVDTFKKTGFTGGINWYRNFSRNWQRAADLDHTVRVPSLMIM
                     AEKDAVLPPSAADGMERIV"
BASE COUNT          579 a         1026 c          962 g          551 t
ORIGIN      
        1 tttctcgcca gcgatctctc gatggcgccg atgcgccatt acctcagcga actcggctac
       61 gacacccacg catggcgcat gggccgcaat accggcggcg tgtcgcgaat gcgcagcgcg
      121 ctgctcgacc ggctgcgtgc ggtcttcgcc gataccggcc gcaaggtctc cctcgtcgga
      181 tggagcctgg gcggcgtcta tgcacgcgat ctggcgctgc aggcgcccga catggtgcga
      241 tatgtgatca ccctggcaag cccgtttgcc agcgacgtga gggcgaccaa cgcgacaagg
      301 ctctacgaat ccgtatccgg cgagacggtc gacctcacct cggagctctc gaagtcgatc
      361 ggcggcgatc tgccggtgcc gaccagcgcg atctattcaa gggccgatgg cgtggtgaac
      421 tggcggacct cccggttgcg cgcctccgcg accgccgaga acatcgaagt gtattttgca
      481 agccatgtcg ggctcggcgt caatccggcg gcgctatggg cggtcgccga tcggctcgcc
      541 caggcggagg gccaattcca tcaatttaac cgattaggac cgtttgccat tgcctatgcg
      601 cccccggagc atgcacaatc cgcccgactg tgaagagcgc gaaaagcgcc gcgcggcata
      661 tttcgggagg agaccatggg tgacggcaaa aaactgtctt cgctggacgc atcgtttctc
      721 tatctggaaa cgccggaaat gccgatgcat gtcggcagca tggcgatctt ccgcctgccg
      781 gacgactaca agggagactt cttcgaagac ttcaaggcga tgatcgcctc gcggctgcac
      841 gttgcgccga tcctgaaggc gcgcctggaa aaggcgccgc tcgacatcga ccatccctcc
      901 tgggtcgagg acgaccagtt cgatatcgat cgccacatct tccgcgccag cctgccggcg
      961 ccgcgcgacc gcgccacgct cgagcgcatc gtcggctgga tgcatgccaa gcttctgaac
     1021 cgcgcccgtc cgctctggga gttctacgtg ttcgagggca tgaaggacaa cgagatcggg
     1081 ctctattcca agatgcatca tgcctgcatc gacggcgggg cgggagcagc gctgaccaac
     1141 atgatctacg acatctcgcc gatcccgcga gaggtcgatc cgccgacggc caaggcgaag
     1201 atcggccagg agccgcgcga catcgcagcc aacctgctgg attcctacca gcagctctgg
     1261 agccagccgc tcgacgccgc cgccgccaga tcgatcgaac tgccgcgctc gggaaagagc
     1321 gatctcggat cgatcctgtt cgacaacgcg atgtaccaga tcgagagcgc ggtacgcttt
     1381 gctggcaaca ttccgaccat gctgaaaagc gtgtccgacg tgatcggcaa gatttccgat
     1441 ccgaaatcgc gcgaaagtct cgccaccatg atgtcgccgc cgaccatcct gaacaaatcg
     1501 atctcgtcgg agcgaagctt tgccggcgtt tcgatttcgc tgtcgcgggc gaaggcgctc
     1561 gccaagcaat cgggcggaaa gctcaacgac gtggtgctgg cgctcgcctc cggcgtggtg
     1621 cggcgctacc tcatcaagca gggcgcgctg ccgaacaagt cgatgacggc agcagtcccg
     1681 atctcgctgc gcgaggaagg caacgccgag gccaacaacc aggtgttcgg catgatctgc
     1741 tcgatcgcca ccaacgttga ggatcccaag gcgcggttgg agacgattat cgcgcaatcc
     1801 accaagtcca aggagatgtc gcatccgctg cgcgcgctga tgccgcaggt ctccaatatc
     1861 tcgatgctgg gcgcgccgat tgtggtgcag attctcgcgc tgctctacag ccgctccagt
     1921 ctgtcggatg tgctgccgcc ggccgccaac atcaccgtct ccaacgtgcc gggaccgcga
     1981 cagacgctct atgccgccgg tgcggaactc ctgcacatct tcccggtttc gatctcgacc
     2041 catgggatcg cgctcaacat caccgtgcag agttaccgcg atcagctcga tttcggcttc
     2101 atcgcgggcg ccaacatcat cccgcacgtg caggagcttt gcgacatgct gccggaggag
     2161 ttttcggcgc tggaagcggc ctacgcgcct ccggcctcgg acatcaaggg cacggccgaa
     2221 taaaggatag cgtaatgatt gaaatgccgc cgcttcagtt cgcgcaaacg aacgggattc
     2281 gcatgggcta ctacgatgcg ggtcccaagt cggacaagcc gccgctggta ttgtgccacg
     2341 gctggccgga gctcgccttc tcctggcgtt accagatcaa ggcgttgagc gaagcgggca
     2401 tccgggtgat cgcgccggac cagcgcggtt atggcgcgac cgaccgcccc gaggcggtcg
     2461 aggcctacga cctcgagcat ctgacccttg acctcgtcgg cctgctcgat catctcggga
     2521 tcgacaaggc ggttttcgtc ggccacgact ggggcggctt tgtcgtctgg cggatggcgc
     2581 tcagccgtcc ctcgcgtgtc gcaggcgtgg tcggcgtcaa cacgccgcat accgatcgcg
     2641 cgcctatcga tccgatcgag ctgttccgca agcgctttgg cgagcaaatg tatatcgtcc
     2701 agttccagga cccggcccgc gagccggacc ggattttcga cggccgcgtc gaagagacct
     2761 tcgacgcctt catgcgcaaa ccgctccagc gctcggacgc cgggccgtcg gagccgccgg
     2821 cggccggcgt cggcgcctcg cccaaactca acctcgcctt tccgcagatg atagcggcct
     2881 atgatgccag gctcgatccg cgcacgccga tcctctcgcc ggaggaaaag caggtgttcg
     2941 tcgacacctt caagaagacc ggcttcaccg gcggcatcaa ctggtatcgc aatttctcgc
     3001 gcaactggca gcgcgcggcc gacctcgacc acaccgtgcg cgtaccgtcg ctgatgatca
     3061 tggcggagaa ggatgcggtg ctgccgccgt ccgccgccga cggcatggag aggatcgt
//
LOCUS       DATLJT010000606         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4401469,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000606 DATLJT010000000
VERSION     DATLJT010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4401469"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..293
                     /locus_tag="VM910_14050"
                     /pseudo
     CDS             75..293
                     /locus_tag="VM910_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
     gene            377..1564
                     /locus_tag="VM910_14055"
     CDS             377..1564
                     /locus_tag="VM910_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63689.1"
                     /translation="MPPVMSLRALICVVLIAAAPAAAAPFVPESDSQVLERLPFSSGD
                     PGLRRLRGLNDQLTRTPNDLPLAILVAQGYAELGRTTGDPRYAGYAQAALAPWWGLER
                     APQEVLVLRAALRQRMHQFDLALTDLATVLNTNPRNVQARLMRATVQQVQGAYDEAKQ
                     ECRALQGLTQELVWTACLGNVNGVTGELRQSYEQLLSVFARYPVAEPKLRSWVLTSLA
                     EMATRAGMMTAAEAHFRAALALDAADFYLLGAYADYLLDDGRPPEVLALLRDKVAADP
                     LLLRYALALQAAQHSQELPARVEQLRDRFAASRLRGDRVHLREEARFTLHLLNAPQEA
                     LKLAQENWQVQKEPADIRILLEAALAANDATAVGIATDWLRNCRLEDMQLSRLMPAAK
                     HQG"
     gene            1614..>2245
                     /locus_tag="VM910_14060"
     CDS             1614..>2245
                     /locus_tag="VM910_14060"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HVI63690.1"
                     /translation="MRHASKLLIPLIAIFVWSSHLPAKAASHREAPLIALDPSADNTD
                     VYAFVSYDAANLARAPADRRVTFILNVNPGQDPSDGPNYFNFGDDVLYAVHIDNDRDG
                     KADDVVYEFRFKTENRPVVAAGDLTSPLPYLGNPHITTALPLQGITALDGPGSEGLTR
                     RQTYTVTEVRNGKRTQLFSGRTLIAVPSNVGPATMPDYPALAAQGIYSDAT"
BASE COUNT          419 a          724 c          704 g          398 t
ORIGIN      
        1 acatccattc gaacgcctgg gacgtagagg ggagtagcgg ccactggctc cgctgcaaac
       61 ccaggagagt ttcgatgaag cgcccagcta tcgctatcac tgccatgatg atcggcctcg
      121 ccatcccggc gatggcgcag acggttcggg tcggcggtca ggcaatgttc cagaacaaaa
      181 cgatcgtcgg taacgcggtg aactccgccg accgcacgac attggccagg gtcaaggccg
      241 ccggtctggt cgacaccctg aatggccctg gcccgttcac ggttttcgcg ccgatcgggt
      301 attctcgcac gggtgcgtcc gtgccggtcg gactgccgta aacgcgatca gccccggcat
      361 cacgaccgag atagatatgc cgccggtcat gtcgctgcgg gcgctgattt gcgttgtgct
      421 catcgcggcg gctccggcgg cggctgcgcc gtttgtcccc gagtccgata gccaggtcct
      481 ggagcggttg ccgttctcgt cgggcgaccc cggcctgcgc aggcttcgcg ggctgaacga
      541 tcaactgacc cgcacgccca acgatttgcc gctggcgatc ctggtcgcgc agggctacgc
      601 ggagctaggc cgcaccacgg gtgatccgcg ttacgccggc tatgcccaag cggcgctcgc
      661 cccgtggtgg ggcctcgagc gagcacccca ggaagtgctg gtcttgcgcg ctgcgttgcg
      721 tcagcggatg catcagttcg atctcgcctt gacggatttg gcgaccgtgc tgaacaccaa
      781 tccgcgcaat gtccaggcgc ggctcatgcg agcgacggtg cagcaggtac agggcgcata
      841 tgatgaagcc aagcaagaat gccgtgcgct gcaaggcttg actcaagaac tggtatggac
      901 tgcttgcctg gggaatgtga acggcgtgac cggtgagctg cgccaaagtt atgagcagct
      961 tctctcagtg ttcgccagat accccgtcgc cgaaccgaag ctccgaagtt gggtgctgac
     1021 cagcctcgcc gaaatggcaa cgcgggccgg catgatgacg gcggcggaag cgcattttcg
     1081 cgcagcgctc gctcttgatg ccgccgactt ctacctcctc ggcgcctacg cggattatct
     1141 gctggacgac ggccgccccc cagaagtcct cgcgctgcta cgggacaagg tcgccgcgga
     1201 tccactgctg ctgcgctacg cactggcgtt gcaggcggcg caacattcgc aggagctgcc
     1261 ggctcgggtg gaacaactgc gcgaccgctt cgcggcgagc cgcctgcgcg gtgaccgggt
     1321 tcacctacgc gaggaagccc ggttcacctt gcatctgctg aacgctccgc aggaggcgct
     1381 gaagctcgcg caggagaact ggcaggtgca gaaggagccg gcggatatcc gcatcctcct
     1441 agaagccgcc cttgccgcta atgatgccac cgcggtcggc atcgcgacag attggctaag
     1501 gaattgccgc ctagaggaca tgcagctcag ccggttgatg ccggcggcaa agcatcaagg
     1561 ttaagaactt ttgtgaccca acccacccgt acgacagagg ggagactgga gatatgagac
     1621 atgcatccaa actactgatc ccattgatcg cgatattcgt gtggtcgtcg catctgcctg
     1681 ccaaggcagc gagccaccgg gaggcgcccc tcatcgccct ggacccatcc gccgacaaca
     1741 ccgacgtcta cgcctttgtc agctatgacg cggcgaactt ggcgcgcgcc ccagcggatc
     1801 ggcgggttac gttcatattg aacgtgaacc cggggcaaga cccgagcgac gggcccaatt
     1861 acttcaactt cggcgacgac gtgctgtacg ccgtccacat cgacaacgac cgggatggca
     1921 aggccgacga cgtcgtctat gagttccgct tcaagaccga gaaccggccg gtcgtcgccg
     1981 ctggtgattt gacttcgccg ctgccgtatc tcggcaaccc gcacattacg actgcactgc
     2041 cgctgcaggg aatcactgcg ctcgatggtc cgggatcgga aggattgact cgtcgccaaa
     2101 cctatactgt gaccgaggtc cgcaacggca agcggaccca gttgttcagc ggccgcacgt
     2161 tgatcgcggt tccctcgaac gtcggaccag ccacgatgcc cgactaccct gccttggccg
     2221 cgcagggcat ctacagtgac gccac
//
LOCUS       DATLJT010000607         5939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2103971,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000607 DATLJT010000000
VERSION     DATLJT010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2103971"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VM910_14065"
     CDS             complement(<1..537)
                     /locus_tag="VM910_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63691.1"
                     /translation="MKPKSIVSLLLGTALTLALAGAASADDKVVKIGSLSDQSGLYAD
                     LSGPGSTVAAQMAVEDSGLTGKGWKIDIISGDHQNKPDIGTSIARQWFDVDKVDIIVD
                     VPNSGVALAVNNVVKEKNGVYINSGAATSDLTNAQCSPNTVHWTYDTYMLAHATGQAL
                     VKAGGDTWFFLTADYAFGQ"
     gene            complement(560..1309)
                     /locus_tag="VM910_14070"
     CDS             complement(560..1309)
                     /locus_tag="VM910_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63692.1"
                     /translation="MPDVNVAPARSSAATTAAPVLSVQNLEAWYGESHILHGMSFDVK
                     AGEVVTLLGRNGAGKTTTLKSIMGIIGKRTGSIRFGTTETIRANSDRIARMGVAFCPE
                     ERGIFASLDVRENLLLPPVVKEGGLTLEQIFDLFPNLKERLKSQGTKLSGGEQQMLAI
                     ARILRTGASFLMLDEPTEGLAPVIIQQIGQTIARLKKEGFTILLVEQNFRFASTVADR
                     YYIVEHGKVIDGFANSELAANMDKLHTYLGV"
     gene            complement(1302..2057)
                     /locus_tag="VM910_14075"
     CDS             complement(1302..2057)
                     /locus_tag="VM910_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63693.1"
                     /translation="MADEFILETHGLTKEFAGFFAVRDVALKVRRGHIHALIGPNGAG
                     KTTCFNLLTKFLKPSAGTIHYKGRDITAMAPADVARLGLVRSFQISAVFPHLTALENV
                     RVALQRQHGSSFDFWRSKTVLNRFNERARELLNDVGLSDFANIPAVEMPYGRKRALEI
                     ATTLALDPEMMLLDEPMAGMGHEDIDKIAALIKRISTKYTILMVEHNLNVVANLSDVI
                     TVLTRGQVLAQGNYAELSKDERVKEAYLGAGHA"
     gene            2407..3009
                     /locus_tag="VM910_14080"
     CDS             2407..3009
                     /locus_tag="VM910_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVI63694.1"
                     /translation="MDFVSANPSVVKSIKQRELLNIWLRLYARNQRMPHVAEYQPERL
                     ADELPDLVYYSVDASHEPPRLTIQSDGTRMSSAYGHTGKGKPLDEYVGARLAPIVMPV
                     YHQCVRRKLPVYTIAHLDDVYGRVVAYERLLLPFSEAEAVTQLIASLKTISEDGEFEI
                     KNLMRGHDAVPRPKLCAVIDRDLFHRRPGRIDAGDVVEFG"
     gene            complement(3006..3389)
                     /locus_tag="VM910_14085"
     CDS             complement(3006..3389)
                     /locus_tag="VM910_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HVI63695.1"
                     /translation="MTITIYGIKNCDTMKKARAWLDSHGVKYDFHDYKSAGIARDKLK
                     AWSDKLGWETLLNRAGTTFRKLPDADKDGLNEKKALALMLAQPSMIKRPVLEAGAKLL
                     VGFAPDIYAKEVAGRARNNDQHAKR"
     gene            complement(3534..4523)
                     /locus_tag="VM910_14090"
     CDS             complement(3534..4523)
                     /locus_tag="VM910_14090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HVI63696.1"
                     /translation="MLVSVEDLERSLDVARAGAAGAREGLFGPASAIWHVNREAAIFL
                     GAGRALLLQLAHPWVAAAIADQSDVFADPLGRFHRTFSVTFTMVFGTLDQATAAARRL
                     YRRHATIVGFLPTVAGPFAAGSPYSANEVSALRWVHATLMETAVLAHDLVLPSLADEQ
                     RERYWAEARLLAALFGIPQASLSPDWASFGAYTRAMVESDVLTVSPAARDIAGQIFSG
                     RVTGLRVPMWYRALTAQLLPEQLRGAFGLPFGDEERRSAGRALRWMARIYPSLPDRLR
                     TVGPYQEAQARVRGEDRLAHATRWLNRLWIGQPSMGSRFPDGAEQRVSQRGKR"
     gene            complement(4616..5320)
                     /locus_tag="VM910_14095"
     CDS             complement(4616..5320)
                     /locus_tag="VM910_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI63697.1"
                     /translation="MSSLSAFPITKRWPPTHPDILQLYSLPTPNGVKVSIMLEEIGLA
                     YEPHLVDFNKDDQKTPEFLSLNPNGKIPAIVDPNGPGGKPLPLFESGAILQYLGEKTG
                     KLLPQDPARRWQTIQWVHFQMGGIGPMFGQVGFFHKFAGKDYEDKRPLQRYVDEAKRL
                     LGVIEQRLAGRQWIMDDEYTIADISMLGWVRNLIGFYGARELVEFDGLEHVPAWLERG
                     LARPAVQRGLNIPKRP"
     gene            5398..>5939
                     /locus_tag="VM910_14100"
     CDS             5398..>5939
                     /locus_tag="VM910_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63698.1"
                     /translation="MNDQTKSGLTDDELAIEPTVFAPDALNNQVFVVSGGAGGIGRAV
                     AWLFARLGAHVALVGRDKSKLDALVAGLASRKLKASAHLADIRDADAVNALFESVFTT
                     NDRLDGLINSAGGQFPQQAIDFSVKGWNAVIDTNLNGTWYMMQAAAQRWRDRKQKGSI
                     VNIVVVTTHGVYGIAHSIAAR"
BASE COUNT         1099 a         1854 c         1858 g         1128 t
ORIGIN      
        1 ctggccgaag gcgtagtcgg cggtgaggaa gaaccaggtg tcgccacccg ctttcaccag
       61 cgcctggccg gtggcgtggg cgagcatgta ggtatcatac gtccagtgca cggtgtttgg
      121 cgagcactgc gcgttagtca ggtcggaggt ggcagccccc gaattgatgt agacgccgtt
      181 cttctccttc accacgttgt tgacggccag cgcgacgccg gaattcggca cgtcgacgat
      241 gatgtccacc ttgtcgacgt cgaaccattg ccgcgcaatc gacgtgccga tgtcgggctt
      301 gttctgatga tcgcccgaga tgatgtcgat cttccacccc ttcccggtca ggccggaatc
      361 ctcgaccgcc atctgggctg cgaccgtcga gccgggcccg ctgagatcgg cgtaaagccc
      421 ggactggtcc gacaacgaac cgatcttgac caccttgtcg tcggcggatg cggcccccgc
      481 gagcgcgagg gtcagcgcgg tcccaagcag gagcgatacg attgatttag gtttcatgca
      541 ggtcctcttg ttgttggtgt cagacaccca gataggtgtg gagtttgtcc atgttggccg
      601 cgagctccga attggcgaag ccgtcgatga ccttgccatg ctcgacgatg taatagcggt
      661 cggcgacggt ggaggcgaaa cggaaattct gctcgaccag gagaatcgta aacccttcct
      721 ttttcagccg cgcaatggtc tgcccgatct gctggatgat gaccggggcc aatccttcgg
      781 tcggctcatc cagcatcaga aagctcgcac cggtgcgcag gatgcgcgcg atcgccagca
      841 tttgctgttc gccgcccgag agtttcgtgc cctggctttt cagacgctcc ttcaagttgg
      901 gaaaaaggtc gaagatctgc tcgagcgtga gaccgccctc cttcaccacc gggggcaaaa
      961 gcaggttctc gcgcacgtcc aggcttgcga aaatgccgcg ctcttcgggg cagaacgcta
     1021 cgcccatccg cgcaatgcga tccgagttcg cgcggatcgt ctcggtcgtc ccgaagcgga
     1081 tcgagccggt gcgcttgccg atgatgccca tgatcgactt cagcgtcgtg gtcttgccgg
     1141 cgccgttgcg cccgagcagg gtcaccacct ccccggcttt cacgtcgaaa ctcatgccgt
     1201 ggaggatgtg ggattcgccg taccaggcct cgagattctg aacgctcaaa accggcgccg
     1261 ccgtcgtcgc ggcggaagat cttgcgggag ccacattcac atcaggcatg accggctccc
     1321 agataggctt ccttgactcg ctcgtctttc gacagctcgg catagttgcc ttgcgcgagc
     1381 acctgaccgc gggtgagcac cgtgatgaca tcggagagat tggcaaccac gttcaaatta
     1441 tgctcgacca tcaggatagt gtatttcgtg gagatgcgct tgatcagcgc ggcgatcttg
     1501 tcgatgtctt cgtggcccat gccggccatc ggctcgtcca gaagcatcat ttccggatcg
     1561 agcgccagcg tcgtcgcgat ttcgagcgcg cgcttgcgac catagggcat ctcgaccgcg
     1621 ggaatattgg cgaagtcgct caagcccaca tcgttcagca gctcccgcgc gcgctcgttg
     1681 aagcgattga gcaccgtttt ggagcgccag aaatcgaagg agctgccgtg ctggcgctgc
     1741 agcgctaccc ggacattctc cagtgcggtc aggtgcggaa acaccgccga gatctggaac
     1801 gagcgtacga gaccgagccg cgcgacatcg gccggcgcca tcgccgtgat gtcccgtccc
     1861 ttgtaatgaa ttgtcccggc cgacggcttt aagaatttgg tcaacagatt gaagcacgtc
     1921 gttttcccgg ccccgttcgg accgatcaac gcgtgaatat gaccccggcg gaccttaagt
     1981 gcgacatcgc gaacggcaaa aaaccccgcg aattccttgg tcaatccgtg ggtctcgaga
     2041 atgaactcgt cggccaaaca ttttccccct gccgcgcacg aacgttcgcg cgtggctctt
     2101 gttgcttacc agcagattgc tccggaacgt cgcaaaaacc tcccggaccg ccgcctccag
     2161 gggcagaata tgccggaggc agaccgggtt aggcaaggcg gaaagctttg gccgctcgcg
     2221 aggcgagctt cgccgttagt cgaactgttg cgattgggtg aaggcggttt cgcccattcc
     2281 ggcaccgccg gggcaagcgg cccggaaaaa ggctcgttaa cgttgtttcg gctggcatat
     2341 ggcattcatg gaaaattttc ctcttccgtc gatcctttgc ggggcttcat cgaactggag
     2401 atcgccttgg atttcgtcag cgccaacccg tcggtcgtga aatcgatcaa gcagcgcgag
     2461 ctcttgaaca tctggctgcg gctctacgcg cgcaaccagc ggatgcctca tgttgccgaa
     2521 taccagcccg aacggctcgc ggacgaactg ccggatctcg tctattacag cgtcgacgca
     2581 tcgcatgagc cgccgcgcct cacgatccag agcgacggca cccgcatgtc gtcggcgtat
     2641 ggccataccg gcaagggcaa gccgctcgac gaatatgtcg gcgcgcgcct cgcgccgatc
     2701 gtgatgccgg tctaccacca gtgcgtgcgg cgaaagcttc ccgtgtatac catcgcacac
     2761 ctcgatgacg tctacggacg ggtcgttgca tatgaacggc tgctattgcc cttctcggag
     2821 gcggaagcgg tgacgcaatt gatcgcctcg ctgaagacca tttccgagga cggcgaattc
     2881 gagatcaaga acctgatgcg cggccatgac gcggtgcccc ggccaaagct ttgcgccgtc
     2941 atcgatcgtg acctgtttca tcgccggccg ggacgcatcg acgcgggcga cgtcgtcgag
     3001 ttcggctagc gtttggcgtg ctggtcgttg tttcgcgctc gtcccgcgac ttccttcgca
     3061 tagatgtcgg gcgcgaagcc gaccagcagc ttggcgccgg cctccagcac cgggcgcttg
     3121 atcatcgagg gctgcgccag catcagggcc agcgccttct tttcgttcag gccatccttg
     3181 tcggcatccg gcagtttgcg gaaggtggtg ccggcgcggt tgagcagggt ctcccagccg
     3241 agcttatcgc tccaggcttt cagcttgtcc ctggcgatgc cggcactctt gtagtcatgg
     3301 aagtcgtatt tgacgccatg gctgtcgagc caggcgcgcg ccttcttcat cgtgtcgcag
     3361 ttcttgatgc cgtagatcgt gatggtcatt tcgtagcagc cccttgagcg ccggcgagat
     3421 cggcggcgat cttccggaga acactgcgcc tgaacaagcg ccggaaccac cctgtccccg
     3481 gatatttcgg accggagaac tgccgggcgt caagcattcg catggtcctc acgtcatcgt
     3541 ttgccacgtt ggctcacgcg ctgctccgcg ccgtcgggga aacgactgcc catgctgggc
     3601 tgtccgatcc agagccggtt gagccatcgc gtggcatgag ccaaccgatc ctcgccccgg
     3661 acgcgcgcct gcgcctcctg atagggaccg acagtgcgca gccggtccgg cagcgagggg
     3721 tagatgcgcg ccatccagcg cagcgcgcgg cctgcgctgc ggcgctcctc gtctccaaac
     3781 ggcagcccga acgcgccgcg caattgctca ggcagcaact gagcggtcaa ggcgcggtac
     3841 cacatcggca cccgaagtcc ggtgacgcgc ccggaaaaga tctgacctgc gatgtcgcgc
     3901 gcggccgggc tgacagtcag cacatcggat tccaccatcg cgcgcgtata ggccccaaac
     3961 gacgcccaat ccggcgaaag gctggcttgg gggatgccga acagggccgc aagcagcctc
     4021 gcctcggccc aatagcgctc gcgctgttcg tcggcgagcg atggcaacac caggtcgtgg
     4081 gccaatacgg ccgtctccat cagcgtcgca tggacccagc gcagcgccga aacctcgttg
     4141 gcgctataag gcgagcccgc ggcaaacggg cctgcgacgg tgggcagaaa tccgacaatc
     4201 gtcgcatgcc gccgatacag ccggcgtgcc gcggccgtgg cctggtcgag cgtgccaaac
     4261 accatcgtga aagtcacact gaaggtccgg tggaagcgtc cgagcggatc ggcaaagaca
     4321 tcggactgat cggcgatcgc ggctgcgacc caggggtgcg cgagctgcag gagcaaggcg
     4381 cggcccgcac caaggaaaat ggcggcttcc cggttgacgt gccagatcgc cgaggcggga
     4441 ccaaacaggc cttcgcgcgc accggccgcg ccggcacgcg cgacgtcgag gctgcgttca
     4501 agatcctcaa ccgatacgag catgacgccc tctatctgag cgcgcatcca ggcgcgcccg
     4561 gccagcggtc agtgtgtgtg ccggatcaga attatgcaac gcgtccagag gtcgctcagg
     4621 ggcgctttgg gatgttcagc ccgcgttgca ccgcagggcg tgcgagcccg cgctccagcc
     4681 aggccggaac gtgctcaaga ccgtcgaatt cgacgagctc gcgtgcgccg taaaagccga
     4741 tcagattgcg cacccagccg agcatggaga tgtcggcgat ggtgtattcg tcgtccatga
     4801 tccactgccg gccggcaagg cgctgctcga tgacgccaag caggcgcttc gcctcgtcca
     4861 catagcgctg cagcggccgc ttgtcctcgt agtctttccc cgcgaatttg tggaagaagc
     4921 cgacctggcc gaacatcggc ccgatcccgc ccatctggaa gtgcacccac tggatggtct
     4981 gccagcggcg cgcgggatcc tgcggcaaca gcttgccggt cttctcgccg agatattgca
     5041 aaatggcacc cgactcgaac aagggaagcg gtttgccgcc gggtccgttc ggatcgacga
     5101 tcgcggggat cttgccgttc ggattgagcg acagaaactc cggcgtcttc tggtcgtcct
     5161 tgttgaaatc gacaagatgc ggctcatagg caagcccgat ctcttccagc atgatcgaca
     5221 ccttgacgcc gttcggcgtc ggcagcgagt aaagctgcag gatatcggga tgagttggcg
     5281 gccaacgctt tgtgatgggg aatgcggaga gagaggacat gaaactcgtt cttggcttaa
     5341 ttcctgacgc aactaatgta tggcgcaccc tcgatgcagc aagaggtctc gtcgccgatg
     5401 aatgatcaga cgaagtcggg tctcaccgac gacgagctcg ccattgagcc gaccgtgttc
     5461 gcgccggacg cgctgaacaa ccaggtgttc gtcgtgtccg gtggcgcggg cggcatcggg
     5521 agagccgtcg cctggctgtt tgcacggttg ggggcccatg tcgcccttgt cggtcgcgac
     5581 aagagcaagc tcgatgcgct ggttgccggt ctcgcctcgc ggaagctgaa agcatccgcc
     5641 cacctcgccg atatcaggga tgcggatgca gtcaatgcgt tgttcgagtc cgtcttcacc
     5701 acaaacgacc ggctcgacgg cctgatcaac agcgccgggg gccagtttcc gcaacaggcg
     5761 atcgattttt cggtcaaggg ctggaatgcc gtgatcgaca ccaacctgaa cggcacctgg
     5821 tacatgatgc aggcggccgc acagcgctgg cgcgatcgca agcagaaggg cagcatcgtc
     5881 aatatcgtcg tggtgacgac gcacggggtt tacggcatcg cgcattcgat tgcggcgcg
//
LOCUS       DATLJT010000608         1747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3712557,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000608 DATLJT010000000
VERSION     DATLJT010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712557"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..563
                     /locus_tag="VM910_14105"
     CDS             342..563
                     /locus_tag="VM910_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVI63699.1"
                     /translation="MSGIVALLLERNPKLTPADIRRILTASAKRLAPGERDDNFGSGL
                     IDPIQALQLADPRSATTTPPAPARPLRQR"
     gene            complement(631..1089)
                     /locus_tag="VM910_14110"
     CDS             complement(631..1089)
                     /locus_tag="VM910_14110"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HVI63700.1"
                     /translation="MQSRLWVGGVLLGLSLSVQTATAGPQEDVAAAGQKWATLFAENN
                     PDTMLPLYAKDAVLWGTLSPTIRSDPAAVKAYFVGAFQALPKATVKFGDQLIRVYGNT
                     AVNSGYYTFSYTKDGETKSIPARYSFTYVKDGNDWKIVDHHSSAVPAPPK"
     gene            1227..1304
                     /gene="pqqA"
                     /locus_tag="VM910_14115"
     CDS             1227..1304
                     /gene="pqqA"
                     /locus_tag="VM910_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HVI63701.1"
                     /translation="MAWSTPTLVEICIGLEINGYLPAEF"
     gene            complement(1444..>1747)
                     /locus_tag="VM910_14120"
     CDS             complement(1444..>1747)
                     /locus_tag="VM910_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007826120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HVI63702.1"
                     /translation="MRQALEATHRGWGQSIIIGVAPAGAEIATRPFQLVTGRVWKGTA
                     FGGARGRTDVPKIVDWYMEGKIEIDPMITHTMPLPDINKGFELMHEGKSIRSVVVY"
BASE COUNT          300 a          559 c          543 g          345 t
ORIGIN      
        1 caccacactt ggaaacccgt gcgttggcaa gcggacgcag cgggaaaccg tcctgagata
       61 tcggcgataa tcgggctgaa agcgttttgt tatgcagcgt gacactttcg ccacgctcgg
      121 cgccccttgc gcttgagccc aacttcaacg tttcggtatg gcggcggggc cgctgggggc
      181 cagggcagca cggtcgatcc gttcatcgcg ctcaacgacc cgacctccgc gtccaatttg
      241 cagcgcctgc gggctgccat tgcgcccctc ggaatagaga tcctggcctg ggaaaacctg
      301 gcaaccaccg gctcgaccgc ggtggcgtcg cggcggccga ggtgagcggc atcgtggcgc
      361 tgctgctgga acgcaatccg aaactgaccc cggctgacat tcgccgcatc ctgaccgcga
      421 gcgcgaagcg gctcgcgccc ggcgagcgcg acgacaattt cggctctggc ctgatcgatc
      481 ccatccaggc gctacagttg gccgatccga ggagcgcgac cacgacgccg cccgccccgg
      541 cgcggccgct ccggcagcgc tagcgggacg cgtttgcggt tttatccggc gcgggcgcaa
      601 tgcgccggcg atctgcgctc acttccgcgc tcacttgggc ggggctggca ccgccgacga
      661 gtggtggtcg acgattttcc agtcgttgcc gtccttcaca taggtgaagc tgtagcgggc
      721 cggaatcgat ttggtttcac cgtctttagt gtaggagaac gtgtaatagc cgctgttgac
      781 cgccgtgttt ccgtagacgc ggatcaactg gtcgccgaac ttgacggttg ccttcggcaa
      841 cgcttggaag gcgccgacga aataggcttt cacggcggcg gggtccgacc gtatcgtcgg
      901 tgacagcgtg ccccacagca ccgcgtcctt ggcataaagg ggcagcatgg tatcggggtt
      961 gttctcggca aagagcgtag cccacttctg ccctgcagcc gcgacatctt cttgcggacc
     1021 cgcggtggcg gtttgcaccg aaaggctcaa ccctagcagg acaccgccga cccaaagacg
     1081 tgattgcatc attccctccc tcatgttcgc agacattcca tcgatgaacc ccgaactgcg
     1141 gacgtgaatt gtagcgatgt tctgcattgc gcgccggccc gctccgattt aacgtattcg
     1201 ttcataaacg ctagggaaga cgaccaatgg cctggagcac cccgaccctc gttgagatct
     1261 gcatcggcct cgagatcaac gggtacctgc cggctgagtt ctgaccccga agttgcgcga
     1321 acctgaacgg gcggcccgtt tggcatagcg ggcggcgcgc gcctgtgttt gcgcatccag
     1381 gcgatcgccg cggtcgtctt cgcttgtaca tcatcatcga ttttttctac acgcaacgag
     1441 cggtcaatac acgacgacgc tcctgatgct cttgccctcg tgcatcaact cgaatccctt
     1501 gttgatgtcg ggcagcggca tggtgtgcgt gatcatcggg tcgatttcga tcttgccttc
     1561 catgtaccag tcgacgattt tcggcacatc ggtgcggccg cgcgccccgc cgaaggccgt
     1621 gcccttccag acgcgtccgg tgaccagctg gaacgggcgc gttgcgatct ccgcgccggc
     1681 aggcgcgaca ccgatgatga tggattggcc ccagccgcga tgggtggctt cgagcgcctg
     1741 acgcatg
//
LOCUS       DATLJT010000609         4767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1414802,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000609 DATLJT010000000
VERSION     DATLJT010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414802"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            759..1757
                     /locus_tag="VM910_14125"
     CDS             759..1757
                     /locus_tag="VM910_14125"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVI63703.1"
                     /translation="MPSDESQATVVSWLLQALRDCLPDSKVIAAGHRVVHGGRLFDRA
                     IVIDDATMAALADLIALAPAHQPHNLGAIHAVAAAWPGLPQVACFDTAFHFGQPRLAR
                     MFGLPRSLSEQGIVRYGFHGLSYEYIASCMSDAAGIGPDQRVVVAHLGHGASLCAIRD
                     GRSVATTMGFTTMDGLVMGKRCGMLDPGVVLYLMREKRLSVAEVDKLLNERSGLFGVS
                     GISDDVRDLEASSDPNAAEALDLFAYRAAREVGSLAAALGGLDALVFTAGIGEHSASM
                     RARICRLSAWAGLVLDDDANAAHRTRISVPGSRVGAFVIPTNEEIVIARAMRTLAL"
     gene            complement(1843..2823)
                     /locus_tag="VM910_14130"
     CDS             complement(1843..2823)
                     /locus_tag="VM910_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HVI63704.1"
                     /translation="MAAVALATPALAADMKVKAPAAPEAPPSPWDVAFGGGIASDYIF
                     RGITQSNHRPSVAAYFEPRYNVTKDLQYYVGIASESISFPNRAAAEVDIFGGFRPTFG
                     PLALDFGGWYYWYPGGQCFNASVNPVFGLDCLANGNLPLNGNVIKRDLSFAEGFAKGT
                     FTVSDQVTLAGAVFYSPSVLNSGAPGTYVSGGAKFTAPSSAMPEGWGFYLSGEAGHWF
                     LGTSDNFYCTQTGVVPGCTAPFPNGIPYRSYTNWNVGIAITRSVFTLDFRYFDTDMNK
                     GDCNAFTSDHTARFSGDFTAINPAGFGSNWCSASFVVTGKFDLTAIANLK"
     gene            3310..3741
                     /locus_tag="VM910_14135"
     CDS             3310..3741
                     /locus_tag="VM910_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI63705.1"
                     /translation="MDGRGFKKWRKQLGFSQEEAAKHFDVTRATIQNWEYEITSLPGA
                     IDRACQECVRRWKQRPDFGPVLLVYTDGLIWAPSDQPDSVPVLYCEPYADNEAAIQRT
                     LRLRDGANFANAWILGEDENVLWNSSELLLECHRRSVRTSV"
     gene            3955..4353
                     /locus_tag="VM910_14140"
     CDS             3955..4353
                     /locus_tag="VM910_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI63706.1"
                     /translation="MNPTDLKSWRKRLGLNQFEAAKKLGVGRASIQYWEQELWPIPRV
                     IELACHQMMRRWKRRPEFGPVLLIFIDDPIWQDSQRPYQVSILHSAVYANNETAMQHV
                     DRVKHDPYFINPVIMETNGDIIWTTFELMR"
BASE COUNT          962 a         1396 c         1454 g          955 t
ORIGIN      
        1 gcagagcttg ctgaacgcgc tagcgccgaa ctgctggtgg ctcatgtgac cgacataggc
       61 gctgctcccg cggaagctgg ctctctcacc acaccacgat atgtcgatca gccacagcat
      121 gagtggcctg cctggaccag cgagtttgtc aagcgccttg catgtatttg tccgctcggt
      181 cacttgcacg tgcggctagt tctcgcccgt ggaagtccgg cggcggaaat cgtgcgcttg
      241 gccaaaaaac aatctacgga cctcgtggtc ctcgcctgga gaggaaagtg ggaagtacca
      301 catgcggcga ctctaaaggc catcttgggt gaggcggggt gccccataat ggtgctgcgg
      361 gcctgacatt tctgcacgct ttgagaccaa cgcgaaataa agcaatgctc gtccaagcgc
      421 caaacaactc gtctacctgg ctggagcgga ttcggcgggc atcgtggtcg gcgcccaggt
      481 gtccatcatg ctgacgagtc gcgccgccgg agaattgagc cggatggcgt cctgtgcgct
      541 cgcccagctg ttcgttcacc acaagagccg gagcccggca tgagcggggc cgcgcgtgcg
      601 ttcctcgtgc tcaatgcggg ctcctcgagc ttgaaatttg gccatacgcc gccgatgatc
      661 tcgtcgccct gtgccgcggc agtatcgagg cgatcgggct cggtgcaacc ttcaagatga
      721 aaggcgccgt cgcaagcgcc ttcgacaagg cgccgccctt gcctagcgac gaaagccagg
      781 ccaccgtcgt gagctggctg ctgcaggcct tgcgcgactg tctgccggac agcaaggtca
      841 tcgcagccgg tcaccgggtg gtccatggcg ggaggctgtt cgaccgggcg atcgtcatcg
      901 acgacgcgac gatggcggcg ctcgccgatt tgatcgccct cgcaccggcg caccagccgc
      961 acaatcttgg cgccatccac gccgttgcgg cggcctggcc cgggctgccg caggtcgcct
     1021 gcttcgacac cgcattccac tttgggcagc cccggctcgc ccggatgttc ggtctgcctc
     1081 gctcgctgag cgagcagggc atcgtgcgct atggctttca tggcttgtcg tacgaatata
     1141 tcgccagctg catgtcggac gcggccggca tcggaccgga tcaacgcgtc gtggtcgcgc
     1201 atctcggcca tggcgccagc ctgtgcgcca ttcgcgacgg gcgcagcgtc gccaccacga
     1261 tgggcttcac gaccatggac gggctcgtta tgggcaaacg ttgcgggatg ctcgatcccg
     1321 gcgtggtgct gtacctgatg cgcgagaagc ggctttccgt cgccgaggtc gacaaacttc
     1381 tcaatgagcg ttcggggctt ttcggcgtgt ccgggatcag cgatgacgtg cgcgacctcg
     1441 aggcgagcag cgacccgaac gcggccgaag cgctcgatct tttcgcctac cgggcggcac
     1501 gggaggtcgg ttcgctcgct gcggcgctcg gtggtctcga cgcgctcgtc tttaccgctg
     1561 gcatcggcga gcactctgcc tcgatgcggg cgcgcatctg ccggctttcg gcgtgggccg
     1621 ggcttgtcct cgacgacgac gccaatgcgg cgcaccggac gcgcatctcc gtcccgggct
     1681 ctcgcgtcgg cgccttcgtc attccgacca acgaggagat cgtgatcgcc cgtgccatgc
     1741 ggacgttggc actgtaacgg gctgggtttc cgtgacagtc gcaccaaagc agcgggcggc
     1801 ggaccgtacg gaaccgccgc ccgcgcacga gccgatatct gcttacttca gattcgctat
     1861 ggccgtcaga tcgaacttgc cggtcacgac gaaagaggcg ctgcaccagt tcgagccgaa
     1921 cccagccgga ttaatcgcgg tgaagtcacc cgagaaccgt gccgtgtggt cgctcgtgaa
     1981 ggcgttgcag tcgcccttgt tcatgtcggt atcgaagtag cggaagtcga gcgtgaacac
     2041 gctgcgggta atggcgatgc cgacgttcca gttggtatag ctgcggtagg gaataccgtt
     2101 cggaaacggc gcggtgcagc cgggaacgac gccggtctgg gtacagtaga agttatcgct
     2161 cgtgcccagg aaccagtggc cggcttcacc ggacaaataa aatccccagc cttccggcat
     2221 ggcgctgctc ggcgccgtga acttcgcgcc gcccgataca taggtgccgg gggcaccgga
     2281 attgagcacg gacggcgagt agaacaccgc accggcgagt gtgacctggt cactcaccgt
     2341 gaaggtgcct ttggcaaagc cctccgcgaa gctcaggtcc cgtttgatga cgttgccgtt
     2401 cagcggtaag ttaccatttg caaggcagtc aaggccgaat accggattga cgctggcgtt
     2461 gaagcactgc ccgcccggat accagtagta ccagccgccg aaatcgagcg cgagcggacc
     2521 gaaggtcggg cggaacccgc cgaagatatc gacctcggca gccgcgcggt tcggaaacga
     2581 gatgctctcg ctcgcaatgc ccacgtagta ctgcaggtcc ttggtgacgt tgtagcgcgg
     2641 ctcgaaatag gcggcgaccg acggcctgtg gttggattgg gtgataccgc ggaagatgta
     2701 gtccgacgcg atccccccgc cgaacgccac gtcccacgga ctcggcggcg cctccggcgc
     2761 tgctggtgcc ttgaccttca tatcggcggc cagtgccggc gtcgcaagcg cgacggcggc
     2821 cactgcagtt aagataaact ttttcatcgg ttgccccttt caatccaacg ccaccccggc
     2881 ggctactgct gctcgtccga tcgcgacttt agtgcttccg cagtcgcgtc ccctggagca
     2941 cgctttcagc taagcaggaa tagaggtctg cggacaatat gagagccgca gcaatcgcct
     3001 aatctttgtg cagatcgcgg tttttcacgt cgaaaaacag caagcgttgc gattcggaca
     3061 cagtctacat accaactgaa actgttaaaa attgatggtc caagccaaca accccttgcg
     3121 cagggacgca cgctgcttcc gtggcccatt gcacggttct ggctgaccgc tcccccgctg
     3181 ggacagtcac gggtgtggat gcttgcgcgg gagcatttga gttatgaatc gatcctgagg
     3241 tttgatcggt ggacagaaga ggttgaacta caagttcgga tggacaagat ttcgaaatga
     3301 gggtggcgaa tggatggacg cggattcaag aaatggcgca aacagctcgg ttttagccaa
     3361 gaagaagcgg ctaagcactt tgacgttaca agggccacga tccaaaactg ggaatatgag
     3421 atcacatctc ttcccggcgc aatcgatcgt gcgtgccagg agtgcgtgcg ccgctggaaa
     3481 caacgacccg acttcggtcc tgtacttctc gtctacaccg acggcctgat ctgggcgcca
     3541 tccgaccaac cggattccgt gccggttctg tattgcgaac cttatgctga taacgaagct
     3601 gcgatacaac ggacgcttcg gttgagggat ggggcaaatt ttgccaacgc ttggatcttg
     3661 ggagaagacg agaatgtcct ctggaacagt tccgaactct tgcttgagtg ccacaggcga
     3721 agcgttcgta cttcggtata gcagcctttg cgtggcgctt aaggaagtct ccagccaaag
     3781 agctcactag gcgcaaggca aaacgaatac tgcgatacgc tgcctcggtt ttcgaaacct
     3841 tgaagtttaa aaggctatgg gagagctcgt tgcggatctc agctgcaaag ccacaaccca
     3901 actcgagttc gaccgcgcta tcaggcattc attggaagaa aaatggttag gagcatgaac
     3961 ccgacggatt tgaaaagttg gcgaaagagg cttggtttga atcaatttga agcggccaag
     4021 aagcttggtg tcggacgtgc aagtatccaa tactgggagc aggagctctg gccgattccg
     4081 cgagtcatcg aattagcatg tcaccaaatg atgcgccgct ggaagcgacg acctgagttc
     4141 ggtcctgtac tcctcatctt catcgacgac ccgatctggc aagactccca aaggccctat
     4201 caggtcagta tcttgcactc tgcagtttat gcgaataacg aaactgccat gcagcatgtc
     4261 gaccgagtta aacacgatcc gtacttcatc aatcccgtca ttatggaaac taatggtgat
     4321 atcatttgga caacctttga gttaatgcgg tagtgtggct cggcctcaaa gtatcgccac
     4381 ggccgcgcag ctggcatcga ggacttgatc gagttaaccg tgagttccgt ggccgtcagt
     4441 cgtcccgcca gcaggacacg aagtcgtgca aaccaaaaga agataagtta gaggcgcgaa
     4501 ggcgcagagc cacgtaacca gaatcaacgt cgaggtgggg gcgtcaactc agtggcgcgc
     4561 cggcacctcg tatcaatgag gcgccactca tctgtacggg ttatcgccca tgtggtggaa
     4621 ttcactcttg ggtccaccac gatagtggtg accaccagtg ctgcgacccg tagtggccgc
     4681 acccttagtc gattttggat gcttcatcga gtgaggcact actgtcttcg gcccgccttg
     4741 gtagtgatga gcattctcat tcttgtc
//
LOCUS       DATLJT010000610         3332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2334114,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000610 DATLJT010000000
VERSION     DATLJT010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2334114"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VM910_14145"
     CDS             complement(<1..429)
                     /locus_tag="VM910_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI63707.1"
                     /translation="MTSDHYDYVIIGAGSAGCVLADRLTEDPSVRLLLLEAGGWDRNP
                     WIHIPLGWPRLFLHRKNDWMYFSEPEPELGSRPIECARGKVVGGSSSINATYVRGHRG
                     DYDRWAASGLLGWSYADVLPYFRRQESWEGGANAYRGGDGP"
     gene            complement(652..1584)
                     /locus_tag="VM910_14150"
     CDS             complement(652..1584)
                     /locus_tag="VM910_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI63708.1"
                     /translation="MTVLDRRAFMAATAASIAAPTALGAKECPTAGMDWITMSLEARN
                     LAYNNVEHVGPENARKKTEGWAAASKTLREQRPKHLDLAYGNGERNKWDLYPANDPQA
                     PCFVHIHGGYWQRGSKEIFACLSEGPLARGWSAALCGYTLAPEASLTQITNELNTAFD
                     WLNAKAGEHGIQGLIIVTGWSAGGHLTSFILDHPKVAAGLAISGVFDLGALRDSPHVN
                     DKVKLSEDEIQKLSPMRRPIVNKPLGIAYGTGELPAMIASSRDYHAYRSQGHVPGDLI
                     PIANTNHFTILDELRQPNSTLTRAVIAMAQYQTA"
     gene            complement(1990..2253)
                     /locus_tag="VM910_14155"
     CDS             complement(1990..2253)
                     /locus_tag="VM910_14155"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63709.1"
                     /translation="MRVRLPLTCEQSSFVAVGCLVSYGPNFGEMHRRAAFYVDRILKG
                     ERPADLPVEQPTKFDLAVNLKAAKALGLEVPLGLSAAADEIIE"
     gene            2264..2815
                     /locus_tag="VM910_14160"
     CDS             2264..2815
                     /locus_tag="VM910_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63710.1"
                     /translation="MSAPRLALKKGDGYGQSQRVRRCRQRAHDDGVLHLGRAYENANP
                     RDGRHRFLDQFESLADKCIISGDPSPGDVSARPRDALDGTAGDWILQPDRDDGNRARD
                     IAHSEQTRLRRSDDHVRLETDQLGGQRWHTFGHALGESQREGYVAALEIAKLAQPRPE
                     TLDVPGCCTCRQWRQNPDERAAV"
     gene            complement(2931..>3332)
                     /locus_tag="VM910_14165"
     CDS             complement(2931..>3332)
                     /locus_tag="VM910_14165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI63711.1"
                     /translation="CGVPTPRSNHARMAAKFALDAIAATGRLRSRLPAPFPIRVGLHS
                     GPVMAGVIGMRRFAYDVWGDTVNIAARLEAAGQPNRVLASATTVKGLGSDYSFDGPHK
                     IDTKEGRMLEAFFVSCHPIDDVSHLPVTVGC"
BASE COUNT          631 a         1056 c         1033 g          612 t
ORIGIN      
        1 cggtccgtcg ccgccgcggt atgcattggc accgccctcc cacgattcct gccgccggaa
       61 atacgggagc acgtccgcgt aggaccagcc aaggagaccc gacgccgccc agcggtcata
      121 gtcgccgcga tggccgcgca cgtaagtcgc gttgatcgag gacgatccgc caacgacttt
      181 gccgcgtgcg cattcgatcg ggcgcgagcc aagctcaggc tcgggctccg agaaatacat
      241 ccagtcgttc ttgcggtgca ggaacaaccg cggccagccc agcgggatgt ggatccaagg
      301 gttgcgatcc cagccgccgg cctccagcag cagcagcctg accgaagggt cttccgtaag
      361 ccgatcggcc agcacgcagc ccgccgaccc cgcgcctatg atgacgtagt cgtaatgatc
      421 cgaggtcacg gctctcccaa gatagcgtca gtccataccg ctttgcggac aatgaattgg
      481 ggcttaccgc gccgacttgt tcctcgacca tctcccgcga tgcgcttgta gcgcgccggc
      541 ccgacgttcg tcgccgatac caactccggc cagggaccga gcttgttcct caagtgtgag
      601 ccaactacct gtgccgggcg agcctcgaag aaagaggatg cgccaaatcc gctacgcggt
      661 ctggtattgc gccatcgcaa tcacggcgcg ggtgagcgtg ctgttgggct gtcgcagctc
      721 atccagaatc gtgaagtgat tggtgttggc gatcggtatc aagtcgcccg ggacatgccc
      781 ttgcgaccga taggcatggt aatcgcggct gctcgcgatc atagccggca actcgccggt
      841 gccgtaggcg atacccagcg gcttgttgac gatcggccgc cgcataggcg agagcttctg
      901 gatctcatcc tcgctcagtt tcaccttgtc gttgacatgc ggagagtcgc gcagcgcacc
      961 gagatcgaac acgcccgaga tcgccagtcc cgccgccacc ttgggatgat caagaatgaa
     1021 cgaggtcaga tgcccgcccg cggaccagcc ggtgacgatt atcagtccct ggatgccgtg
     1081 ctccccggcc ttggcgttga gccagtcgaa cgcggtgttc agctcgttgg tgatctgcgt
     1141 caggctcgcc tccggcgcca gcgtgtagcc gcacagagcc gccgaccaac cacgggccaa
     1201 cggaccctcg gagaggcagg cgaaaatctc tttgctgccg cgctgccaat agccgccgtg
     1261 gatgtgaacg aagcatggcg cctgcggatc attggcagga tataggtccc acttgttgcg
     1321 ctctccgttt ccataagcca agtcgaggtg cttcgggcgc tgctcgcgca gcgtcttcga
     1381 agctgcggcc cagccttcgg ttttcttgcg cgcgttctcg ggacccacat gctcgacatt
     1441 gttgtatgcg agattgcggg cctcgagcga catcgtaatc cagtccatgc cggcagtggg
     1501 gcattccttc gcaccaagag cggttggtgc cgcaatgcta gcggcagtcg ccgccatgaa
     1561 ggcgcgacga tcaagcactg tcatatgaac ctcccggatt ttatttaagc tcggcggcgg
     1621 ccaagccaac aacatcgccg accgagcacc cccaacgcta gtcttcttgc cgaactgtgt
     1681 aaaccttccg cgatactaca aggacaattg gcgagcttcg ccctctttgc gcacgtctgg
     1741 gacgtgcctt tgcgggattt ctcatccggc tatgtcggct cagactcggt tattcggcga
     1801 tgtctgatca atgtccggat tatcccacaa gcggacctcg gcgcgtcatc acggcatgtc
     1861 gcatgagtgc cataaccggc tgtgagcaat cgcagcaaac gttgctattc gatcctcgtc
     1921 agcgagcgcc gagcagggtc ggcggaagcg cgataccgag ggtttgttcc gcgcgggcta
     1981 tagggtcttt cattcaatga tctcgtcggc ggcggcagaa agccccagag gcacttcgag
     2041 gccgagcgcc ttagcggcct tgaggttcac ggcaagatcg aatttggtcg gctgctcgac
     2101 cggcaggtcg gcggggcgct cgcctttgag aatgcggtcg acgtaaaagg cggcgcgacg
     2161 gtgcatttcg ccgaaattgg ggccatagga aaccagacag ccaaccgcga cgaacgaaga
     2221 ctgctcgcag gtcagcggca atcggacgcg catcgcttgt ccaatgagcg ccccgcgatt
     2281 ggcgctgaag aagggtgatg gttacggtca atcccagcgc gtgcgccggt gccgacaacg
     2341 ggcgcatgat gacggagtcc tgcacctcgg tagggcttac gagaacgcca atccgcgcga
     2401 tggtcggcac cgcttccttg atcaattcga gtcgcttgcc gacaagtgca tcatctccgg
     2461 cgatccaagt cccggtgacg tgtccgctcg gccgcgcgat gctctcgacg gcaccgctgg
     2521 cgactggatc ctgcagccag atcgtgatga tgggaatcgt gcgcgtgaca ttgcgcacag
     2581 cgagcaaact cgtctccggc gcagcgacga tcacgtccgg cttgagaccg accaactcgg
     2641 tggccagcgc tggcatacgt tcgggcatgc cctcggcgaa tcgcagcgcg agggttacgt
     2701 tgcggccctc gaaatagcca agctcgcgca gccccgccct gaaaccctcg atgttccggg
     2761 ttgctgtact tgccgacagt ggagacagaa tcccgatgag cgggccgccg tctgaagatt
     2821 gccgcgcacg cacggcaagg ggccacgcgg ccgcggcccc accgaggagc gctatgaact
     2881 cgcgccgctg catgtcggac attggcgttc cttcctcccg ggagtgacca tcagcaaccg
     2941 acggtgaccg gaagatgtga gacatcatca atagggtgac aacttacgaa aaatgcctcc
     3001 agcatgcggc cttccttggt gtcgatcttg tgtggaccat cgaagctgta atcgcttccc
     3061 agccctttca cggtcgtcgc ggacgcgagc acgcgattgg gctgaccggc agcctcgaga
     3121 cgcgcggcga tgttgacggt atcgccccag acgtcgtagg cgaaccggcg catgccgatg
     3181 acccctgcca tcacgggacc ggaatgcagg cccacccgga tcggaaacgg ggccggcagc
     3241 cgagaccgca atcgcccggt ggcagcaatc gcgtcgaggg cgaattttgc cgccatccgg
     3301 gcgtgattgg agcgtggcgt cggcacgccg ca
//
LOCUS       DATLJT010000611         3649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3712755,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000611 DATLJT010000000
VERSION     DATLJT010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712755"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VM910_14170"
     CDS             complement(<1..344)
                     /locus_tag="VM910_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63712.1"
                     /translation="MAKDKQKTRRPKKQKTRRPKRVISGEIDEANISVPKPKKFSTNK
                     FKSKRAAAMANVETLLTALPHHSISQAKDFVRLHPDEEKYWSPELCFVNVPVKGQKRD
                     TLHLIDEELAMR"
     gene            complement(598..1341)
                     /locus_tag="VM910_14175"
     CDS             complement(598..1341)
                     /locus_tag="VM910_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63713.1"
                     /translation="MYKGNDHQKQDDEAGLTQVNLDPVTRGQEAWGRLRKSLADWFIV
                     AEALHHGQHLAMLEARTNKPVGARFQAVMGEWLRVTGFHEIDKGVRSRLLNCLQHREK
                     IEAWQKTLPLDKRQQFNHPNVIWRAWRKSTVIGRATGIARLSPTAKYKDEIARLENEN
                     HILRRAGDDLFTATDAAIDIAQLLADRLLRLTPNKARQILELLPELYAKRSAETPHDK
                     ARPRARTKRRTVEDFQRDLAARKAGAGTK"
     gene            complement(1491..1712)
                     /locus_tag="VM910_14180"
     CDS             complement(1491..1712)
                     /locus_tag="VM910_14180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI63714.1"
                     /translation="MNKHFRTGKKRRYRRRPPTAASLYHSPDEFAERARIGRATVWRL
                     MKQGRLRYARFGRARRIPVTEYQRLTSDA"
     gene            complement(1820..2146)
                     /locus_tag="VM910_14185"
     CDS             complement(1820..2146)
                     /locus_tag="VM910_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63715.1"
                     /translation="MARRRLPPPIRGEVVRVIEMDDSEPIARVKVEWPEEPPRPLTQR
                     QQMIVAITRRRWRGRITVSIAEVLRHVRTVWEDECRRRKLPEPWPPVPNRDTVARALR
                     AASLID"
     gene            complement(2139..3326)
                     /locus_tag="VM910_14190"
     CDS             complement(2139..3326)
                     /locus_tag="VM910_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI63716.1"
                     /translation="MLRAKRGWHNDGGGLYLRVEDKHRRWWVYRYGAGGKHYHGLGPA
                     HTIGLADAREQARRCRQLLLEGADPIAAGKARRAAVRLAAATAKTLADCVEAYHAAHC
                     AGWTNQKHAREWKASLATHVLPEIGTLPVAAIDTAAVMRVLEPAWTKIPETAARLRSR
                     IEQVLDWARVRGYREGENPARWRGHLDHLLPARKKLAPVKHHAAMHYRDVPAFIRELR
                     QRDEVEARALELLILTASRAGEVCGAEWREIELADVGWSWRRPPERMKMRREHRVPLS
                     RAARAVLERTPTDRRHGRIFPGVSGHMLWKFLRTLIDATTVHGFRSSFRDWAAEQTNF
                     AREAAELALAHRVGDDVEEAYLRSELLAKRRQLAEAWARYCASVPAARSDNVASIRGG
                     ARG"
     gene            complement(3426..>3649)
                     /locus_tag="VM910_14195"
     CDS             complement(3426..>3649)
                     /locus_tag="VM910_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63717.1"
                     /translation="RVFFGCKMNLYIDYGMKLNYKLRQPRSSRGVSGDDPEGGARHRL
                     SNARKLLALLANRRKIPFPLMLGQSPTVY"
BASE COUNT          645 a         1149 c         1087 g          768 t
ORIGIN      
        1 tatcgcatcg ccaattcttc gtcgatgagg tgcaacgtgt cacgcttctg gcctttaacc
       61 ggcacgttga caaagcaaag ctccggcgac cagtactttt cctcgtcagg atggagcctg
      121 acaaagtcct tggcttgcga gatcgaatga tgcggtagcg cggtcagtaa ggtttctacg
      181 ttagccattg ctgctgcacg cttggacttg aacttattcg tgctgaattt tttcggcttg
      241 ggtacagata tgttcgcctc atcgatctcg ccggagatca ctcgcttagg tctgcgggtt
      301 ttctgtttct taggtctgcg ggttttctgt ttatcttttg ccattttcgc ctccttctga
      361 tttcaaattc tcaactccag aaaacaagca cgtgctcgtt gtcggagtcc tcgaagtcgt
      421 cggcagtgct gaaaccttgc gtccacgctc cgcctttgcc gtcagcacag cgcacaaacc
      481 agcagccggg gccgaggtct gcaccgtcct agcagcagca aactctcgca cctgaacgtt
      541 catgatcgca gctccaggtc ggcaaagatt cgtcggcttt gatagatatc gcgcatttca
      601 ctttgtccct gcgccagcct tgcgcgctgc aagatcgcgt tggaaatctt cgaccgttcg
      661 gcgcttcgtc cgtgcacgcg gtcgcgcttt atcgtgcggt gtttccgccg agcgcttggc
      721 gtacaattcg ggaagcagct cgagaatttg cctcgccttg ttcggcgtca atcgcagcaa
      781 gcgatcggcg agcaactgcg caatatcaat cgcggcatct gttgcggtga acagatcgtc
      841 cccggcgcgg cgcagaatat ggttctcgtt ttcgagacgc gcaatctcgt ccttgtactt
      901 tgcagtgggg gaaaggcgcg cgatgccggt tgccctgccg atgacggtcg actttcgcca
      961 cgcgcgccaa ataacattcg gatgattaaa ttgctggcgc ttgtccaaag gtaacgtttt
     1021 ctgccacgcc tcgatctttt cccgatgttg aagacaattg agcaggcgag aacggactcc
     1081 tttatcgatt tcgtggaagc cggtcacacg caaccactca cccatgacgg cttgaaaacg
     1141 cgctccgacc ggcttgttgg tacgggcttc caacatcgcc aggtgttgac cgtgatgaag
     1201 cgcttcggca acgatgaacc agtcggccaa actttttcta aggcggcccc atgcctcttg
     1261 cccacgggtg accggatcta ggttgacctg ggtcaaccca gcttcatcgt cttgcttttg
     1321 atggtcattc cccttataca ccgacacacc tccgatcatt tgagcaatgc gacgaggttc
     1381 gtttctggtg acggctcagg ccctgaacat cctgagccgc cactctcgta aacgccgcac
     1441 tgtgcagatc ttcccggcgc cggcggcggc gagaccgggg cgggagcctg tcacgcgtcc
     1501 gaagtcaggc gttgatattc ggtcaccgga atccgtcgag cgcgtccgaa gcgcgcatac
     1561 cgaagcctcc cttgcttcat cagtcgccac accgtcgcgc ggccgatgcg tgcgcgctcg
     1621 gcaaattcgt ctggagagtg gtagaggctg gcggccgtcg ggggtcggcg acgataacgg
     1681 cgcttcttgc cggtgcggaa atgtttgttc actgagtcgc tccaaatctt gggacgaccc
     1741 tcgattaatc tcacaatctc atggagcaaa tgcgcaagtg cacgcatgtg tacgcaaata
     1801 tacgcatgcg caattcgcgt tagtcgatta atgaagcggc gcgtagagcg cgggcaactg
     1861 tgtcgcggtt aggcaccggt ggccatggtt ctgggagctt ccgccgccgg cattcgtcct
     1921 cccagacggt gcgaacgtgt ctgagcacct ccgcgatact gacggttatc cgaccgcgcc
     1981 agcgacgccg ggtgatcgcc acgatcattt gctgccgctg cgtcagcggc cggggcggct
     2041 cttccggcca ttcgactttt acccgcgcga tcggctcgct gtcgtccatc tcgatcaccc
     2101 tgacgacctc gcccctgatc gggggcggca agcgccgtct agccacgggc gcctcccctg
     2161 atcgacgcga cgttgtcgga cctcgccgcc ggaacactcg cgcagtagcg cgcccaggcc
     2221 tccgcaagct gccggcgctt agcgagcagc tcgctgcgga gatacgcctc ctcaacgtcg
     2281 tcgccaacgc gatgggcaag tgccaactcg gccgcctcgc gggcgaagtt tgtctgctcg
     2341 gcggcccagt cacggaacga gctgcgaaaa ccgtgcaccg tggtggcgtc gatcagcgtg
     2401 cgcaggaatt tccacagcat gtgtccgctc acgccgggga agatgcgccc atgacgtctg
     2461 tccgtcggcg tccgctccaa cacggcgcgc gccgcccgag ataagggaac gcggtgctcg
     2521 cgccgcattt tcatgcgttc gggcggccgt cgccacgacc agccgacgtc ggctagctcg
     2581 atctctctcc actcagcgcc acagacttcg ccggcgcggc tcgcggtcaa gatcagcaat
     2641 tccaacgccc tcgcctcgac ctcgtcgcgc tggcgcagct cacgtatgaa agccggcacg
     2701 tcgcggtagt gcatggcggc atggtgcttg acgggggcga gcttcttgcg ggcgggtaag
     2761 agatgatcga gatggccgcg ccagcgggcc gggttctcgc cctcgcggta gccacggacc
     2821 cgcgcccagt cgagcacttg ctcgatccgc gagcgcagcc gcgctgccgt ctccgggatc
     2881 ttggtccagg ccggctccag cacgcgcatc accgccgccg tgtcgatcgc cgccaccggc
     2941 agggtgccga tctccgggag cacatgggtc gcgagcgagg ctttccactc tcgcgcgtgc
     3001 ttctgattgg tccagccggc gcaatgcgct gcgtgatagg cctcgacgca gtcggccagt
     3061 gtcttggcgg tggcggcggc gagcctgacg gcagcgcggc gggccttgcc ggcggcgatc
     3121 ggatcggcgc cttcgagcag cagttgacgg cagcggcgtg cctgctcgcg cgcgtcggcc
     3181 agaccgatgg tgtgggccgg cccgaggccg tgatagtgtt tcccgccggc gccgtagcga
     3241 tagacccacc agcggcgatg cttgtcctcg acgcgcagat agaggccgcc gccgtcgttg
     3301 tgccagccac gtttcgcgcg cagcaccttc aattcggtca gccgcttgat cgatctggcc
     3361 atagccagcc cgtccacatc tccgtccaca ctcgcgcttg agattgagcg agacggggcg
     3421 agatgtcaat agacagtggg agattgacca agcataagcg gaaaggggat tttccgccta
     3481 ttagcgagca gcgccaacaa cttgcgggca ttactcaagc ggtgcctcgc tccgccttcg
     3541 ggatcgtctc cggagacgcc cctcgatgag cgaggctgac gaagcttata attcagcttc
     3601 ataccgtagt caatatataa attcattttg cagccaaaaa acacgcggc
//
LOCUS       DATLJT010000612         4908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_955102,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000612 DATLJT010000000
VERSION     DATLJT010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955102"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="VM910_14200"
     CDS             <1..2292
                     /locus_tag="VM910_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63718.1"
                     /translation="SVQTTAATSMKLLRFPTTWAELALRPPEFILVIFGMCVLLIVEW
                     LLETRLATTINGSHFVNSDGRMADAVVRTGYQFAGFLKLTNLNPVQGFGSQLLPINIW
                     INPVHWPLAFIDDDKLATDIAGLVGLTCVAAACYFMARCFDLSPLSSIISAQLSLVLF
                     GPLGPLLALTASFVLLTGMAAVYAPYIAALGLLARLDPGRVGDFVWYTAGITALIFYS
                     LCCDSLWSVVGATSWVVPFAVVALSPLRLKAILIRCAALGLTLALLLVSGVLLYVYTL
                     TQYTSRVFFPARMERPFAPGYASMLFTSPNGKYLYGLCLLGWVVGLASAAGRVRVLVV
                     AGSAAFAFFVLYLLAYMVLDVTWWLPLPLYVEHSLAPLFMVSAFAGLWSGGQLIVTRI
                     RVPSVTIPSLTIPSRAINARRLAPRSWILFLAGLAALLLVPLAGMAYSFERAKIVLAR
                     YYTTPWVNEPELVDYLVHSTGLSVGQTYRGSSLGLTVDTESLANLWKRGVPTLDEYSQ
                     LVTPQAFYLQAALLGSPPDLNSFGLGVRIAPDVNIELIFKTLQALGVRYIFNPSPLAE
                     ADQRQVPSAQVPRRPHNALPRDEPGIWHIYELPSPNIGNYSPTEVVVADSARDILAIL
                     GTKDFDFRRRVVLPSGSPSLVPARVTRLTINRGGGFHVFGKSDGVSLILLPQQFSHCM
                     KASDSNVRIVRADLLWTGVIFSGTVDTDISFDYGMFSPGCRRADIADLTRLGIAVPVP
                     PATGRQGVTEKFQVTIDAIKSVW"
     gene            complement(2629..2895)
                     /locus_tag="VM910_14205"
     CDS             complement(2629..2895)
                     /locus_tag="VM910_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63719.1"
                     /translation="MARIDAGGGRVVNRDIVPPGEQGRLYVGPPPPGPDDLKPRSTAA
                     IDGKAVPPGTTDAEREANRQRVNNDRSAYTQQGLRPAGGEPWRK"
     gene            2894..3877
                     /locus_tag="VM910_14210"
     CDS             2894..3877
                     /locus_tag="VM910_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI63720.1"
                     /translation="MPRPMSPPRLVLDRVRRSWFIRDHYAFIRLGVPEHERAAAEKRL
                     GEYLAQKHVPEVGPNPWVADVLGVYAQERLPQTRAARKAAYNALSLIKFWGTMRVNEI
                     TPSNCLAYAANRTPAAARRDLEVLSAALRWWHKHREPLAPLPSLVMPPRPEPRERWLT
                     REEAARLLWASRHVEHLKRFILLGLYTGSRSGVILSLEWSWIDLERGVMRRRARGQAE
                     TSTKRTPPVRLGRKILGFLRRWKQGSEGLVVHYRGQRLQELGSSWITACARAGLKDVS
                     PHTLRHTRATWLMQQGVDLWQAAGHLGMSPGVLQKTYGHHHPDYQGDAAEV"
     gene            complement(3874..>4908)
                     /locus_tag="VM910_14215"
     CDS             complement(3874..>4908)
                     /locus_tag="VM910_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVI63721.1"
                     /translation="LGWIFAASWVAGGADAAWQGLAFLVLLFACVVAHEFGHIFTARA
                     FGVSTPDVTLLPIGGVARLERIPEEPYEEFLVAIAGPLVNVAIAVALILIAGARVNAG
                     DLYAVESPHTSMIDRLAAVNLFLAVFNMIPAFPMDGGRVLRALLAARMGYVRATEIAA
                     FIGQGFAFALGFIGLFFNPLLIFIAIFVYLAASSEAHLVAIRAMSRGVPTSAAMMTQF
                     ATLAPDAHVEEAVQTLLRTSQGEFPVVDGAGKPVGLLGRGDLIRALKQLGPDARVADA
                     MTTTVPTIGHRRCLEEAFRMLQEKSAPAVAVVDGSGRLVGLVTSETIAALLSLRHKGL
                     QVPRHSFGTD"
BASE COUNT          861 a         1534 c         1518 g          995 t
ORIGIN      
        1 tctgttcaga caaccgcagc cacctcaatg aagctccttc gttttccgac aacgtgggcc
       61 gagcttgctc tgcgtccgcc ggagttcatc ctcgttattt tcggaatgtg cgtgctactg
      121 attgtcgagt ggctgctcga gacgcgtctt gccaccacta tcaatggcag tcacttcgtc
      181 aatagcgacg gcaggatggc ggacgctgtg gtgcggacgg gctaccagtt cgccggattt
      241 ttgaagctga ccaacctcaa tccggtccag ggattcgggt cacaattgct tccgatcaac
      301 atttggatca acccggtcca ttggccgctt gccttcatcg acgacgacaa attggcgacg
      361 gatattgcag ggttggtggg actgacctgc gtcgcggcgg cttgctactt tatggcccgc
      421 tgcttcgatc tgtcaccgct gtcgagcatt atctccgcgc aactcagtct ggtcttattt
      481 gggccactcg gaccgttgct ggccctcacc gccagcttcg tcctcttgac tgggatggca
      541 gccgtctacg ctccatacat tgcggcactc ggtctgcttg cgcgcctcga tcccgggagg
      601 gttggagatt tcgtctggta caccgccgga atcacggccc tgatattcta cagtctatgc
      661 tgtgactcgc tctggagtgt cgtcggtgcg acaagctggg tggtgccgtt tgcggtggta
      721 gcactgagcc ctctacgctt aaaagccatc ctgattcgtt gcgcagcact tggcctcact
      781 ctcgcgcttc tgcttgttag cggtgtattg ctctacgttt acacactcac gcagtatacc
      841 tcgcgcgttt tctttcccgc ccgcatggag cgcccgttcg ctccgggtta tgcctcgatg
      901 ttgttcacct cgccgaatgg aaaatatttg tatggacttt gcctgctggg ctgggtcgtc
      961 ggccttgcct ctgctgcggg tcgggtacgc gtgcttgttg tagctggttc agctgctttc
     1021 gctttctttg tactttacct tcttgcatat atggtgctgg atgtgacgtg gtggctgccg
     1081 ctgcctttgt atgtcgagca cagcctggct ccattgttca tggtctcggc attcgccggc
     1141 ctttggagcg gcggacaatt gatcgtcact cggatccgcg taccttcagt aaccatccct
     1201 tcactaacca tcccctcccg tgccatcaac gcgcgacgtc tcgcgccgcg gtcttggatc
     1261 ctttttctcg ccggtcttgc cgccttgctg ctggtaccgc tggctggaat ggcgtactcg
     1321 ttcgagcgcg cgaaaatcgt gctggctcgc tattacacga cgccatgggt gaatgagccg
     1381 gaactggtgg attatcttgt ccactccaca ggactttcgg ttggacagac atatcgcggc
     1441 tcgtcgttgg gattgacggt cgacaccgaa tcgctggcga atctctggaa gcgaggggtg
     1501 ccgactctcg atgaatacag ccagctcgtc acgccgcagg cattctatct gcaagccgca
     1561 ctattgggat cgccgccaga tctcaacagc ttcggccttg gcgtgaggat cgcccctgac
     1621 gtaaatattg agcttatatt caagacgctc caggccctcg gggtccgtta tatcttcaat
     1681 cccagcccat tggctgaggc cgatcaacgg caggtaccca gtgcccaggt tccgcgccga
     1741 ccacacaatg ctttgccgag agacgaacca ggcatctggc atatttatga gcttcctagt
     1801 cccaatatcg gcaattacag tccaaccgag gtcgtcgtcg ccgattcggc gcgggacatt
     1861 cttgcaatcc tcggtacgaa agatttcgac ttcaggcgcc gcgtcgtcct gcccagtggc
     1921 agcccgagcc tcgttcccgc tcgcgtcaca cgcctcacga taaaccgtgg tggcggcttc
     1981 cacgtcttcg gaaagagcga tggagtatcc ctaatccttc tcccgcagca gttcagtcac
     2041 tgcatgaagg cttccgacag caatgttcgc atcgtgcgag ccgacttatt gtggacgggc
     2101 gtgatctttt cgggcaccgt cgatacggac atctcgtttg actacggcat gttctctccc
     2161 ggatgtcggc gtgctgatat tgccgacctg acccggcttg gaattgccgt tccagtcccg
     2221 cctgcaactg ggcggcaagg cgtcacggag aaattccagg tcaccatcga cgccataaaa
     2281 tccgtctggt agaggcgtct ccgatcgcgc aggagcgatg ggtgcctggg cgaagctaat
     2341 gcctttgcaa gcagttacgc caaacgcaca aagtcgcgca gcaattgctg ggcgatcggg
     2401 tggcccttgg gcaaaatcgc cgccctcgtg ctcgaccgga aaaagttggc tgcattttcg
     2461 gccgccgccg gtcgatttct gcagttcccg attggcgata ggcgctgtaa ccgaacgcgc
     2521 gcgagagcgt tggcaatgcc ggcgggaacg cgcgcccaag ccttacaaac gcgactccag
     2581 cggcgtcgcg cctgcttcac cgcaggtcgc ctagacactc ctgcttcatc acttccgcca
     2641 cggctcaccg cccgcgggcc gcaagccttg ctgcgtgtag gctgagcggt cgttgttcac
     2701 gcgctgacgg ttcgcttcgc gctcagcgtc ggtcgtcccg ggcgggaccg ccttgccgtc
     2761 gatggcggcc gtgctccgcg gcttcagatc atcgggaccg ggaggaggag ggccgacgta
     2821 gagccggcct tgctcgccgg gcgggacgat gtcacggttg acaactcgcc caccgccagc
     2881 gtcaatgcgc gccatgcctc gcccaatgag cccgccgcga ctggtattgg accgagtacg
     2941 ccggtcctgg ttcatcagag atcactacgc cttcattcgg ctcggcgtgc ccgaacacga
     3001 gcgcgccgcg gccgagaagc ggcttggcga atatctcgcg caaaagcacg tgcccgaagt
     3061 cgggccgaat ccgtgggtgg ccgatgtgtt gggcgtgtac gcgcaggagc gactcccgca
     3121 gactagggct gcgcgcaagg ccgcgtacaa tgctcttagc ctgatcaagt tttggggcac
     3181 gatgcgcgtc aatgagatca cgccttcaaa ctgtctggcc tacgcagcca acagaacgcc
     3241 agccgcggcg cgccgagacc ttgaagtgtt gagtgcggcg ctccgatggt ggcacaagca
     3301 tcgggagccg ctcgcgccgc tcccgtcatt ggttatgccc ccgaggcccg agccgcgcga
     3361 gcggtggctc acacgcgagg aggcggcaag attgctgtgg gcgtcgcgcc acgtcgagca
     3421 cctcaagcgt tttatactgc tcgggctcta cacgggctcg cggtcgggcg tgatcctgtc
     3481 gcttgaatgg agctggatcg acttagagcg cggcgtcatg cgccggcgcg cacgggggca
     3541 ggcagagacc tctaccaaga gaacgccgcc ggtgcgccta ggcagaaaga ttctgggctt
     3601 tctacgtcgg tggaagcaag gctctgaggg cctcgtcgtg cactaccgcg gccagcggct
     3661 gcaggagttg ggcagctcgt ggattacggc gtgcgccagg gcggggctga aagacgttag
     3721 cccgcacacc ttgcggcaca cccgggcgac gtggctaatg cagcagggcg tcgacctgtg
     3781 gcaggcggcg gggcacttgg ggatgtctcc gggagtcctc cagaagacgt acgggcacca
     3841 tcacccggac taccaggggg acgcggccga ggtctaatct gtgccgaagc tgtgccgcgg
     3901 aacttgtaag cctttgtgcc gtaatgataa aagagcagct atcgtctcgg aggtgacgag
     3961 accgacgagg cgtccgctgc catcgaccac ggcgaccgcg ggcgccgatt tttcctgcaa
     4021 catgcggaac gcctcttcca ggcagcggcg atgaccgatg gtcggcaccg tggtcgtcat
     4081 cgcgtcggcg acgcgggcgt cagggccgag ttgcttgagc gcacgaatga gatcgccgcg
     4141 gccgaggagg ccgaccggct tgcccgcccc gtcgaccacg ggaaactcgc cctggctcgt
     4201 gcgcagcagc gtctgcaccg cctcctccac gtgcgcatcg ggcgcaagcg tggcgaattg
     4261 cgtcatcatc gccgcgctcg tcggcacgcc ccgtgacatt gcccggatgg cgacgagatg
     4321 ggcttccgag gaggccgcga gatagacgaa gatcgcgatg aagatcagta gcgggttgaa
     4381 gaacagcccg atgaaaccga gcgcgaaggc gaagccttgg ccgatgaacg cggcgatttc
     4441 ggtcgcgcgc acgtagccca tgcgcgcggc gagcaacgcg cgcagcaccc gcccgccgtc
     4501 catgggaaag gccgggatca tgttgaatac ggcgaggaac aggttcacgg cggcaagccg
     4561 atcgatcatc gaggtgtgcg gactctcgac cgcgtagaga tcgcccgcgt tgacccgcgc
     4621 gcccgcgatc aggatcaagg cgaccgcgat cgccacgttc accagcgggc cggcgatcgc
     4681 caccaggaat tcctcatagg gctcctccgg gatgcgctcg aggcgggcca cgccgccgat
     4741 cggcagcagc gtcacgtcgg gtgtcgacac gccgaaggcg cgcgcggtga agatgtggcc
     4801 gaattcgtgc gccaccacgc acgcgaacag cagcaccagg aaagccagcc cttgccaggc
     4861 cgcgtccgct ccgcccgcga cccagctggc ggcgaagatc cagcccag
//
LOCUS       DATLJT010000613         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_725257,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000613 DATLJT010000000
VERSION     DATLJT010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725257"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..>2304)
                     /locus_tag="VM910_14220"
     CDS             complement(211..>2304)
                     /locus_tag="VM910_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63722.1"
                     /translation="YSTELAFPPFDPDDVLNPGAPLTLTLDYVDVADIATLAPPVAGS
                     ARLPVPRARDVRLRLTPRCADKPNYFGTQSVGGLAVDVRDGLTVDVGTRAPAQSEAGL
                     FAAQVPEVELNGILLQPAPDMLIRLANHLGLAADGLTLTARPGERVLFGASAALRHSL
                     SGDGSALTFASDGELLGHWLAVIRLTLDRDWTWDGIDPEGFVVSRRDLPAELPADPPR
                     EVGGLQMPFAVSGIALSGPDQPGVDRRASTRLIFLDAVDPQPKAGEFPEIKIPEWVVE
                     PRLLGLPEAANVALRRTLSIQLPVAAKPRQTPKLVSAGIALSPYQHDNIYASTEPRQR
                     VLWFEFEEPIADPNDALFARVTAYGPDPLLSGAITHLLIPAPAIPLGPTTWFDLIEAL
                     LPTPPAPPALAIDPEPMRVIVPDQPEDSSGLDAMKEMEAAIPPAPATPLRHYILPLPP
                     AIAPDAPDLFGFWTYELRVGHNKIWSTAQARFGRPLEVKGVQHPPPGMRCTAFRFREA
                     KTAPRRIVAMAPHATAVFQDKRLTDPAGANDPRTRIWVLLYAQVMQADGATRRNVLIA
                     RAPAIPQLDAVNGKPIPPQTRDVMGVAQFDEPSIEQKLADLALPSDSPLSVLAVELLP
                     SDHLVQQTFTIGDAQVYFTFDQPDPFADPRQPVFGGFIAAPPEVSDPLGRELGKMTSR
                     RILRCSPLTPVAPAC"
BASE COUNT          415 a          724 c          801 g          364 t
ORIGIN      
        1 ccaaaaaccc ccgtcgccga tccgaggccg atcgtggtgg ggcgatcgcg accggggtag
       61 tcaaggagtc cggcggtccg gggctgagcg ccgcggcgtg tggggctcag gcccagtctg
      121 gaaagcgacc gacaaccaag cggcaatgat ggcgacaagc cattgtcgaa caggtcttgt
      181 tgtaggggct ctcctccgat tacacggggg tcagcaagcc ggcgcgaccg gcgtgagcgg
      241 agaacagcgc aggatacggc ggcttgtcat tttgccaagc tcgcgcccga gcggatcgct
      301 cacctccggc ggcgcagcga tgaatcctcc gaacacaggc tgccggggat ccgcgaacgg
      361 gtcgggctga tcgaacgtga agtacacctg cgcatcgccg atcgtaaagg tctgctgcac
      421 caggtgatca ctgggcagca actcgactgc gaggaccgag agcggcgagt cggagggcag
      481 cgcgaggtcg gcgagcttct gctcaatgct cggttcgtcg aactgggcaa cccccatgac
      541 atcgcgtgtt tgcggcggga tcggtttgcc gttcaccgca tcgagctgcg ggatcgccgg
      601 cgcgcgggcg atgagaacat tccggcgggt cgcgccgtcg gcctgcatca cttgcgcata
      661 caacagcacc caaatgcgcg tgcgtggatc gttggcaccg gccgggtcgg tcagacgttt
      721 gtcctgaaac acggcggttg cgtgcggcgc catcgcgacg atgcgacgcg gcgcagtttt
      781 tgcctcgcga aaacggaatg ctgtgcaacg catccctggc ggaggatgct gcacgccttt
      841 cacctccagc ggccggccga aacgcgcttg cgcggtggac cagatcttgt tgtggccgac
      901 gcgaagctcg taggtccaga agccgaaaag gtccggcgcg tcgggagcga ttgccggcgg
      961 cagcggcaag atgtaatggc gcagcggggt cgcgggcgcc ggcggaatcg ccgcctccat
     1021 ctccttcata gcgtcgaggc ccgagctgtc ctcgggttgg tccggcacga tgacgcgcat
     1081 cggctccgga tcgatcgcga gcgccggcgg cgcgggcggc gtcggcagca gggcttcgat
     1141 caggtcgaac catgtggttg gtccgagcgg tatcgccggc gccggtatca aaagatgcgt
     1201 aatcgcaccc gacagcagcg gatcggggcc gtaggccgtg acgcgtgcga acagcgcatc
     1261 gttgggatcc gcgatcggct cctcgaactc gaaccaaagc acgcgctgcc gcggctcggt
     1321 cgacgcataa atattgtcgt gctgataagg cgatagcgcg atgcccgcgg acaccagctt
     1381 cggtgtctgc ctcggttttg ccgcgaccgg caattgaatc gaaagcgtac gccgaagcgc
     1441 cacgttcgcg gcctcgggca gaccgagcag tcggggctcg accacccact ccggtatctt
     1501 gatctccggg aattcgccag ccttcggctg cggatcgact gcatccagga agatgagccg
     1561 cgtggaggcg cgtcgatcga cgccaggctg gtcgggaccg gacagcgcga tgccggatac
     1621 cgcaaagggc atctgcagtc cgccgacttc gcgcggcgga tcggccggta gctcggcggg
     1681 tagatcgcgc cgactcacga cgaagccttc ggggtcgatg ccgtcccagg tccagtcgcg
     1741 gtcgagcgtc agccgaatga ccgcgagcca gtgtccgagc aattcgccgt ccgacgcaaa
     1801 ggtgagcgcg ctgccatcgc ccgacagcga gtgacgaagc gcggccgatg cgccgaacaa
     1861 tacgcgctcg ccgggccgcg cagtcaacgt gagtccgtcg gcggcgagac cgagatggtt
     1921 cgccaaccgg atcagcatgt cgggcgccgg ctgcagcaag atcccgttta actcgacttc
     1981 tggtacctgc gcggcgaaca atcccgcttc cgattgcgcc ggtgcgcggg tgccgacgtc
     2041 cacggtcaat ccatcgcgaa cgtcgacggc caatccgccg acggactgag tgccgaaata
     2101 attcggcttg tccgcgcagc gcggcgtcaa tcgcaggcgc acgtcgcgcg cgcgcggcac
     2161 aggcaacctc gctgaccctg ctacgggggg cgcgagcgtc gcgatgtcgg cgacatcgac
     2221 ataatcgagc gtcaacgtca gcggtgcgcc ggggttgagc acatcgtcgg gatcgaacgg
     2281 aggaaatgcg agctccgtgc tgta
//
LOCUS       DATLJT010000614         4710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_725329,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000614 DATLJT010000000
VERSION     DATLJT010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725329"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VM910_14225"
     CDS             complement(<1..984)
                     /locus_tag="VM910_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI63723.1"
                     /translation="MHGTIDLAQNAELEAARERAFSLPLEEFNPGDPELFRSDTHWPY
                     FDRLRKEEPVHYCKDSIFGPYWSVTRYNDIMDIDTNHMVFSSASVLGGITIRDVSPDL
                     RRESFISMDQPRHSAQRKTVAPMFTPTNLDELAVNIRKRSAGCLDHLPVNEVFDFVDQ
                     VAIELTTQMLAVLFDFPWEDRRKLTRWSDVAITLPGPGGLVATEDERQAELVECAQYF
                     AKLWNERIEGPPKNDLLSMMAHNEATHDMDPKNFLGNLILLIVGGNDTTRNTMSGSVY
                     ALSKHPDQYRKLRENPALLDSFVPEVIRWQTPLAHMRRTALQDIEFRGKTIK"
     gene            1219..2391
                     /locus_tag="VM910_14230"
     CDS             1219..2391
                     /locus_tag="VM910_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HVI63724.1"
                     /translation="MIPNAYRMFNFDLGETADALRETVQNFASHEIAPRAAEIDKTNQ
                     FPRDLWPRIGALGLHGITVEEDYGGSGLGYLEHCVAMEEISRASAAVGLSYGAHSNLC
                     VNQIRRNGSDAQKRKYLPKLISGEHVGSLAMSEPNSGSDVVSMSTRAEKKGDRYVLNG
                     SKMWITNGPVSETLVVYAKTDPNAGPRGITAFIIEKGMKGFSTAQKLDKLGMRGSDTA
                     ELVFDNCEVPEENVLGDVGKGVNVLMSGLDYERAVLAAGPLGIMQACMDVVLPYVHER
                     KQFGEPIGSFQLVQGKLADMYTTMNASRAYVYAVAKACDRGETTREDAAGAILYAAEK
                     ATHVALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKTA"
     gene            complement(2446..3243)
                     /locus_tag="VM910_14235"
                     /pseudo
     CDS             complement(2446..3243)
                     /locus_tag="VM910_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
     gene            complement(3299..3778)
                     /locus_tag="VM910_14240"
     CDS             complement(3299..3778)
                     /locus_tag="VM910_14240"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI63725.1"
                     /translation="MSGGMGFFGRGAGPIPRQTVYYPTNYAAGTIIINTAERRLYLVQ
                     PGGQALRYGIGVGRDGFRWSGVHRISAKREWPGWTPPVEMRRRRPDLPGHMVGGIENP
                     LGARALYLGSTLYRIHGSNEPETIGQAVSSGCFRMTNEDVVDLYNRVSIGATVVVRN"
     gene            4117..>4710
                     /locus_tag="VM910_14245"
     CDS             4117..>4710
                     /locus_tag="VM910_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI63726.1"
                     /translation="MNGAESLVRTLVAGGVNVCFTNPGTSEMHFVAALDRVEGMRCVL
                     GLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGLANLHNAKKAHSGTVNIVGQHAT
                     YHIGYNAPLTSDIEGLARPMSEWVRTSPNAKSVAADGAAAIAAARSSPPQIATLILPA
                     DTAWNEADGIAQVPPPGQKPGFSTEAVETAAKVLHGDA"
BASE COUNT          856 a         1470 c         1525 g          859 t
ORIGIN      
        1 cttgatcgtc ttgccacgaa attcgatgtc ttgcagcgcc gtgcggcgca tatgggcaag
       61 cggcgtctgc cagcggatca cttcgggcac gaagctgtcg agcagcgccg ggttctcgcg
      121 cagcttccgg tactgatcgg gatgcttgct cagcgcgtag acgctgcccg acatggtgtt
      181 tcgcgtggtg tcgttgccgc caacgatcag aaggatcagg ttgccgagga agttcttggg
      241 gtccatgtcg tgggtcgctt cattgtgcgc catcatcgac aacaggtcgt tcttcggcgg
      301 accctcgatg cgctcgttcc agagcttcgc gaaatattgc gcgcattcca caagttcggc
      361 ctgccgctcg tcctcggtcg caaccaggcc gcccggaccc ggcagcgtga tcgcgacgtc
      421 ggaccagcgg gtcagcttgc ggcgatcctc ccaggggaag tcgaacagca ccgccagcat
      481 ctgcgtcgtc agctcgatcg cgacctggtc gacgaagtcg aatacttcgt tgacgggcag
      541 atggtcgagg cagcccgccg accgcttgcg gatgttgacc gcaagctcgt cgagattggt
      601 cggcgtgaac atcggcgcca cggtcttgcg ctgcgccgag tggcgcggct ggtccatcga
      661 gatgaagctt tcgcggcgca ggtccggcga cacgtcgcgg atggtgatgc cgcccagcac
      721 cgaagccgag gagaacacca tgtgattggt gtcgatgtcc atgatgtcgt tgtatcgcgt
      781 cacggaccag tacgggccga agatcgagtc cttgcagtaa tggaccggct cctccttgcg
      841 cagacggtcg aaatagggcc agtgcgtatc agagcgaaac agttcgggat cgccgggatt
      901 gaactcctcg agcggcagcg aaaaagcccg ctctcgcgcc gcctccaact ccgcattctg
      961 ggcgagatcg atggtcccgt gcatgggcgg ctccctgttg atcccttcct cgtgtcggga
     1021 aggtggcttt gcgcgaatta catcccgttg cgcccgcccg tgcaagggag agtttgccac
     1081 aggacaccgt gccttgccgg ggttatccgg gctggtatcc cactcggcgg catattcggc
     1141 ccgtgacaag cgcattttcg cggaataagg ctgtaagccg aaaacaagcg acagtgatgc
     1201 ctggaggctg ccaagacgat gatccccaat gcgtatcgga tgttcaattt cgatctcggc
     1261 gagaccgcgg atgcgcttcg cgagacggtg cagaatttcg caagccacga gattgcgccg
     1321 cgtgccgccg aaatcgacaa gaccaaccag tttccccgcg acctctggcc gagaatcggc
     1381 gcgcttggcc tgcacggcat cacggtcgag gaggattatg gcggctcggg tctcggctat
     1441 ctcgaacact gcgtcgccat ggaagagatc tcgcgcgctt ccgccgcggt cggcctgtct
     1501 tacggcgcgc attccaatct ctgcgtcaac cagattcgcc gcaacggcag cgacgcgcag
     1561 aagcggaaat atctgccgaa gctgatttcg ggcgagcatg tcggctcgct tgcgatgtcg
     1621 gagccgaact cgggctcgga cgtcgtgtcg atgtccaccc gcgccgagaa gaagggcgac
     1681 cgctacgtcc tgaacggcag caagatgtgg atcaccaatg gcccggtctc tgagacgctg
     1741 gtggtctatg ccaagaccga tcccaatgcc ggccctcgcg ggatcacggc cttcatcatc
     1801 gaaaagggca tgaagggatt ttcgaccgca cagaagctcg acaagctcgg catgcgcggc
     1861 tccgacaccg ccgaactggt gttcgacaat tgcgaggtgc cggaagagaa cgtgctcggc
     1921 gacgtcggta agggcgtcaa tgtcctgatg tccggcctcg attacgagcg cgcggtgctt
     1981 gcggcgggtc cgctcggcat catgcaggcc tgcatggacg tcgtgctgcc ttacgtgcac
     2041 gagcgcaagc agttcggcga gccgatcggc agctttcagc tcgtgcaggg caagctcgcc
     2101 gacatgtaca ccacgatgaa cgcgtcccgc gcctacgtct atgcggtcgc gaaagcgtgc
     2161 gatcgcggcg agaccacgcg cgaggacgct gcgggcgcca tcctgtatgc cgcggagaag
     2221 gcgacgcacg tcgcactcga cgcgatccag cttctcggcg gcaacggcta tatcaacgac
     2281 tatccgaccg ggcggctgtt gcgcgacgcc aagctttacg agatcggcgc cggcaccagc
     2341 gagattcggc gcatgctgat cgggcgcgag ctgttcgaga agacggcgta ggcagacacc
     2401 gtcattccgg gttcacgctt tcgtgccgcg gagtgacgcg tcgcttcact ccggcagatc
     2461 gttcacctgt ttcacccacg cccgcacgtc ggcgagcacc tccggcagca ccactttgac
     2521 ttcctcgacc gtcatgccgg ccttgatgcg ggggtcgtag agcaggttga ggatgtactg
     2581 gtcgaacacg tcgaagaatc ccatcgaaac cttgtcgttg aacatggtcc agggcaccga
     2641 gtcggtgtcg ttgatcggcc cgagcgactg caacagctcc tcataggcgc aatcgaaaaa
     2701 gacgaagtcg ccattgtcga ccgtgaggat cacgtcggaa tgctcgatct cgtaattttc
     2761 gttcttgcgg aagccggaca ggcattgcgg atcgagcgag gcccggatct cgcgcgcgcg
     2821 ttcgttgccg tagaacgcgg caatcgtgcg gttgaggtcg cggtcgcgca ccagcctgac
     2881 gttgatggtg gcgtccgcgc tggtgtcggc cattgcgatg tcgagatgct gaacgcgcct
     2941 tttgatgtcg gccacgacct gcgcgagctg cgccttgcgg tcggcgcgcg tgccgtcgac
     3001 gaacacgcgc accggtccgt tatattttcg gatgcggtcg atgcggccgg cgaggtgata
     3061 ttcggtaccg aaggcggttt tcagaaaacc gtcggcaatt tcggcatcgg tgaagaccta
     3121 gcgctccacg cgcttgcgtg cggcgatggc gggaatttcc gccgacgccg aaggcggtgc
     3181 gaccgccgcg atcgcgaaag ccgcaattgc agcgagcaaa gacaggcggt gaatggcgtc
     3241 catcgtcctg cgacgctgcc gcacccggcc gccgcgcgca aggcgcaggg gcaagcgctc
     3301 agttcctgac gacgacggtt gcgccgatcg agacgcggtt atagaggtcg accacgtcct
     3361 cattggtcat gcggaagcag cccgaggata ccgcttgccc gatggtttcg ggctcgttgg
     3421 agccgtggat gcggtagagc gtcgagccga gatagagcgc ccgtgcgccg agcgggtttt
     3481 cgatgccgcc caccatgtgg cccggcaggt cgggccggcg ccggcgcatc tcgacgggcg
     3541 gcgtccagcc cggccattcc ctcttggcgc tgatccgatg cacgccgctc cagcgaaagc
     3601 cgtcgcgccc gacgcctatg ccgtagcgca gcgcctggcc gccgggctgc acgagataga
     3661 gccgccgctc ggccgtgttg atgatgatgg ttcccgccgc gtagttggtg ggatagtaaa
     3721 cggtttgacg cgggatcggg cccgcgccgc ggccgaagaa acccatccct ccgctcatga
     3781 tgtcgccggc gaagccttgg gcgtgcacgc tgccaagccc tgcaaggctt gccacgacag
     3841 cagccaacac gagaacagcc cgggtcattt tacctcctcg cgaaaatcgt cgggggcgtc
     3901 gctcaataga ggccatattg gccgcctctc cgcaagttac ggccatgtga caggcggggg
     3961 tttccaacgc ctccgttaac gagctctgca tcccggctcg agagcgatgt tgccgccccc
     4021 tttgacgaaa tcatcgaaca atgattgatc tggccccccg gggggatgcg aatattggaa
     4081 tcggatcaca agcggtaagc aaacgggagc ggaacgatga atggtgcgga aagcctggtg
     4141 cggaccctgg tcgcaggcgg cgttaacgtc tgcttcacca atcccggcac ctccgagatg
     4201 catttcgtcg ctgctctcga ccgggtcgaa ggcatgcgct gcgtgctcgg actgttcgag
     4261 ggcgtggtga cgggcgccgc cgacggctat ttccgcatga aaggcacgcc ggcctcgacg
     4321 cttctccatc tcggccccgg gctcgccaac gggcttgcca acctgcacaa cgccaaaaag
     4381 gcgcattccg gcaccgtcaa catcgtcggc cagcacgcga cctatcacat tggctacaac
     4441 gcgccgctca cctcggatat cgagggcctg gcgcggccga tgtcggaatg ggtacgcacc
     4501 tcgccgaacg ccaaatcggt ggccgccgac ggtgccgccg ccattgccgc cgcaaggagt
     4561 tcgccgcccc agatcgctac cctgatcctg ccggccgata ccgcgtggaa cgaggccgat
     4621 ggaatcgcgc aggtgccgcc gccgggccag aagccgggct tttccacgga agctgtcgag
     4681 acggcggcca aggtgttgca tggcgatgcc
//
LOCUS       DATLJT010000615         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3023711,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000615 DATLJT010000000
VERSION     DATLJT010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023711"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VM910_14250"
     CDS             complement(<1..546)
                     /locus_tag="VM910_14250"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63727.1"
                     /translation="MQIRVTVIAAGGGTVSALAAKAATTMIPVVFISDDHPVKVGLVA
                     SLNRPGGNVTGIHQFTTGLEAKRLGLLHELVPNATTIAVLVNPDYPDADTQIREVQAA
                     ARALGVQVEVLKASGERDFDTAFATLIQQRAGALLVASDPFLFSRRDQLVAQAARHPV
                     PAIYQFRESAVSGGLMSYGTHI"
     gene            complement(531..659)
                     /locus_tag="VM910_14255"
     CDS             complement(531..659)
                     /locus_tag="VM910_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63728.1"
                     /translation="MASYIRRRKFLATLLGGAPAAWPLAVDMTDCRRWLPSWCKFG"
     gene            984..1217
                     /locus_tag="VM910_14260"
     CDS             984..1217
                     /locus_tag="VM910_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63729.1"
                     /translation="MLGIGSGSFDPETLVVLEAAFDEAWITLKTNGSGNIKPNELARR
                     ICHLAMEGERDPVRLHDRALMRLIPATIWRELS"
     gene            complement(1566..>2898)
                     /locus_tag="VM910_14265"
     CDS             complement(1566..>2898)
                     /locus_tag="VM910_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI63730.1"
                     /translation="LVPFEPPDHPTIRAAFRNVIESYPRALYDGQGLVRYRTRFLDSV
                     LVSEPELIHDVLVARADLFRRDDVTRQLLSPMLGPTSLFMAEGADWKWQRRAVAPTFQ
                     PDKLLTLVPTFSTIAVRQVERWRAAPQEQPVDVAEAMAQTTLEVIVGTVLGSAGALDA
                     QGFARALTTELDSFPWRFILTAFRAPLWLPYPGRWRAARAREHIYREAARLVAQRRRV
                     SARADLLDLLLRARDEETGRVMTDEELVRNLATFILAGHETTAVALTWTLWLLAKYPH
                     VQDQVAAEAQATIGSGAVEAQQVERLAFTRQVLQEAMRLYPPAPAISRQPLAPLLLGG
                     EQLTPATQVTITIYPLHRNRLVWKNPDSFDPNRFTVDQAKARPRYAYLPFGAGPRVCI
                     GASFAMIEATVVLAILVRAFRFNTVTGHRPHPVARVTLRPRGGMPLYIEPR"
BASE COUNT          584 a          872 c          921 g          521 t
ORIGIN      
        1 gatgtgggtt ccgtagctca tcaggccgcc ggacacggcg gactccctaa actggtagat
       61 cgcgggcacc ggatggcgcg ctgcctgggc gaccagctgg tcacgccggc tgaacaagaa
      121 cggatcggag gcgacaagca gtgcgccggc ccgctgttgg atgagagttg caaaggcggt
      181 gtcgaaatca cgttcgccgc tggccttcag gacctcgacc tgtactccga gggcgcgcgc
      241 agcggcctgt acctctctta tctgggtgtc cgcatctgga taatccgggt tcacgagcac
      301 ggcgatcgtc gtggcgttag gaaccagttc gtgcagtagc ccaagcctct ttgcctccag
      361 gccggtcgtg aactggtgga tgcccgttac gttgccgccc ggccggttga ggctcgcaac
      421 gaggccgact ttgaccgggt ggtcgtcgga aataaagacg accggaatca tcgtggttgc
      481 cgccttggcc gcgagtgccg acaccgtacc gccgcccgca gcaattactg tcacccgaat
      541 ttgcaccagc tcggcagcca gcgccgacag tcggtcatat cgaccgcgag cggccatgcg
      601 gccggcgcgc cgccgagaag cgtggctagg aattttcgtc gtcggatgta gcttgccatg
      661 tgaccctccg gttgggggtc attcatgcaa tgagggatca taccacgatt ttccgtctga
      721 atcgttgccg aatagatcaa acaggattga atcaactccc ggcgctcaag ccatcgagga
      781 ggtgttatgc cccacagtac gatagcgggc gtgatgacca gcgctcccgc cagatcaccc
      841 atccaccatg tcgtccctat cgatcgaaaa tcagaccact ctgcgtaacc gcccaaactc
      901 aagctgccga ccccgattcg actacttttt gagggaggct caaagacgtt gtttcctagc
      961 aacctcgatc gtggagaggg aacatgcttg gaatcggcag tgggtctttt gatccagaga
     1021 cactcgtagt cttggaagcc gcctttgatg aagcgtggat aactctcaag actaacggca
     1081 gcggtaatat caagcccaac gaactcgcgc gacgaatctg ccatcttgcg atggaaggag
     1141 agcgtgatcc cgtgcgactg catgaccggg cacttatgcg gctgatcccg gcaacgattt
     1201 ggcgcgaatt gagctaacga ctgccgcgtc ttcgggggtg gaaaggcgaa gggccgcgcg
     1261 aggccaaggc ccttgggcgc ccgcggtgtt ttcgtgcccg caggagccgg gtacgcgcgt
     1321 cggtcggtgc agaagagttc gaaattgccc actgccgtca caccggggcg gctcgcgcag
     1381 ggtcagacgc ttgcagcacc ggagatgccg cgcttggccg gcttccgcgc ttcggcaaga
     1441 actatcagcg cgggctcagg ctcttcgaaa cccgccggtg ccttgtcgcg ctcgaaatgg
     1501 ttggttgtgc cgtttggaga ataggtcgca tagggcaagc gcaccaactg tcataggccg
     1561 gaacttcacc gcggctcgat gtaaaggggc atgccgccgc gcggccgcag cgtgacacgt
     1621 gcgaccgggt gcggccgatg cccggtgacc gtattgaaac gaaaggcgcg aaccaaaatt
     1681 gccagaacga cagtcgcctc gatcatcgcg aagctggcgc cgatgcagac gcgcggcccc
     1741 gcgccaaacg gtaggtaagc ataccgcggg cgcgccttgg cctggtccac cgtgaagcgg
     1801 ttaggatcaa agctatcggg atttttccag acgaggcgat tgcgatgaag ggggtaaatc
     1861 gtaatggtga cctgcgtggc cggagtcaac tgttcaccgc caagcaggag tggagctagc
     1921 ggctgacgtg aaatggccgg tgccggcgga tataatcgca tcgcttcctg aagcacctgc
     1981 cgcgtgaacg cgagccgttc gacctgctgc gcctcgacgg caccgctgcc aatagtagcc
     2041 tgagcctctg ccgcgacctg gtcctgcacg tggggatact tggccagcag ccatagtgtc
     2101 caggtcagcg ccacggctgt cgtttcatgt ccggcgagga tgaaggtggc gagattgcgc
     2161 accagctcct cgtcggtcat cacacggcca gtttcctcat cgcgtgcccg cagcagcaga
     2221 tcgagaagat cggcgcgggc ggacacccga cgccgttgcg caacgaggcg cgcggcctca
     2281 cgataaatgt gctcgcgcgc tcgcgcggcg cgccaccggc ccggataggg cagccagagc
     2341 ggcgcgcgga atgcggttaa gatgaaccgc cacggaaacg aatcaagctc ggtggtcaat
     2401 gcacgcgcga atccttgcgc atcgagagca ccggcactgc cgagcaccgt gccgacaatc
     2461 acctcgagcg tcgtttgcgc catagcctcg gcaacatcga ctggttgctc ctgtggcgcc
     2521 gctcgccagc gttcgacctg ccgcaccgcg atcgtgctga atgtagggac caaagtgagc
     2581 agcttgtcgg gttggaaagt aggcgcgaca gcgcgccgct gccatttcca atccgcacct
     2641 tccgccatga acaacgaggt ggggccgagc attggtgaca acagctgccg cgtcacgtcg
     2701 tctctgcgaa agaggtccgc ccgcgccaca agaacgtcgt gaatgagctc gggctcactg
     2761 acaaggactg aatccaagaa ccgagttcgg tatcgtacca agccctgccc atcatagagc
     2821 gcgcggggat agctctcgat gacattgcga aatgccgcgc ggatggtcgg atgatctggc
     2881 ggttcgaagg gaacgaga
//
LOCUS       DATLJT010000616         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3253263,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000616 DATLJT010000000
VERSION     DATLJT010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253263"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VM910_14270"
     CDS             complement(<1..192)
                     /locus_tag="VM910_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HVI63731.1"
                     /translation="MAEYVVEFGKDGSPVEADGRLDAEAFHRNHQPIWAVLQRYLDGK
                     SGDVLETGSGTGQHAVHFAA"
     gene            354..701
                     /gene="soxX"
                     /locus_tag="VM910_14275"
     CDS             354..701
                     /gene="soxX"
                     /locus_tag="VM910_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HVI63732.1"
                     /translation="MILLPKSALVAAIALALSAASAHAQSAAAEGKKLAFDRGKGNCL
                     SCHEIRGGDLPGTIAPPLADLKAKYPDRADLVAIVTDESKRNALTVMPPFGRNRILTE
                     QEINAIVDFLQTL"
     gene            727..1218
                     /gene="soxY"
                     /locus_tag="VM910_14280"
     CDS             727..1218
                     /gene="soxY"
                     /locus_tag="VM910_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HVI63733.1"
                     /translation="MTTDESLPLSRRLVLKGAGAVALIGLGNLALLPMPAIAAANDKY
                     PENAFKQKSDADAIKALYGRTAEASDKVKLDVPEIAENGSVVPVSVTTTLAGVTSISF
                     FVSENPNALAASYVIPEGTLPHVANRLKMAKTCNVIAIVESEGKLFSATKEVKVTVGG
                     CGG"
     gene            1293..1604
                     /gene="soxZ"
                     /locus_tag="VM910_14285"
     CDS             1293..1604
                     /gene="soxZ"
                     /locus_tag="VM910_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HVI63734.1"
                     /translation="MASTIRVRAISNGEVTEVQALIQHPMDTGLVKDAKGDLIPAHFI
                     QQLTFEHNGRTVFLADWGPAVARDPYVKFSFKGAAKGDDLKIAWVDNTGATDTMMAKI
                     Q"
     gene            1607..2461
                     /gene="soxA"
                     /locus_tag="VM910_14290"
     CDS             1607..2461
                     /gene="soxA"
                     /locus_tag="VM910_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HVI63735.1"
                     /translation="MRVRLLLQSGLAALALAIFIQPATAADTPDPAADAKAFRDYFVK
                     KFPKVKLEDFVNGPYSLNEDMRRQWEEKEQFPPYEFALEAGKEMFATPFRNGKTYADC
                     FPNGGIGIRQNYPYFDEKEGKVVTLELALNRCREANGEAPYSYVKDEMASLTAYMAFT
                     SRGKPMDIKVPNDPRALAAYEDGKRYFYTRRGQLNFSCATCHVQSPGERIRAEILAPA
                     YGILNAMPIYRSEWNGMGTISRRFVTCNSQTRAVPLEPQSDEYRDVEYFLSYVANGLP
                     IAGPGARP"
BASE COUNT          516 a          788 c          791 g          490 t
ORIGIN      
        1 cgccgcaaaa tgcaccgcat gctggccggt gccgctgccg gtctccagca cgtcgccgga
       61 tttgccgtca agataccgct gcagcacggc ccagatcggc tgatggttgc gatgaaacgc
      121 ctccgcatca agccggccgt ccgcctcgac ggggctgcca tccttgccga attcgacgac
      181 atactccgcc atcccgggct cccgtcgcgc gcttgcggaa ccgccctcgt cctttgcgct
      241 gcggcaggca ttttaaccgc attttgcggc ctagaaacgc cggattgcca gaagatggcc
      301 tttgtgcttt tcaccaccta tattttttca tgaccacatc cccggacatg cgcgtgatcc
      361 tgcttcccaa atccgcactg gtcgcggcaa tcgcgctggc gctctcggcc gcttccgctc
      421 acgcgcaatc cgctgccgcg gaggggaaaa agcttgcgtt cgatcgcggc aagggcaatt
      481 gcctgagttg tcacgagatc aggggtggcg atctgcccgg cacgatcgcg ccgcccttgg
      541 ccgacctcaa agccaaatat cccgatcgcg cggatctggt tgcgatcgtc accgacgaga
      601 gcaagcgcaa cgcgctgacc gtgatgccac cgttcgggcg caaccgcatc ctgaccgagc
      661 aggagatcaa cgcgatcgtg gactttttgc aaaccttgtg acgtcacgtt ttttggagat
      721 ttgttgatga cgacagacga aagccttccc ctctcgcgcc gcctggtgct gaaaggcgcc
      781 ggcgccgttg cgctgatcgg gctgggcaac cttgcgcttt tgcccatgcc ggcaattgcc
      841 gccgccaatg acaagtatcc ggaaaacgcg ttcaagcaga agagcgacgc ggatgcgatc
      901 aaggcgcttt acgggcgaac ggcggaagcc tcggacaagg tgaagctcga tgtgccggag
      961 attgccgaaa acggttcggt ggtgccggtg tcggtgacga cgacgcttgc cggcgtcacc
     1021 tcgatctcgt tctttgtcag cgagaacccg aacgcgctgg ccgcgtccta tgtgattccg
     1081 gaaggtacgc tgccgcatgt cgccaaccgg ctgaagatgg cgaagacctg caacgtgatc
     1141 gcgatcgtcg aatccgaggg caagctgttt agcgcgacca aggaagtcaa ggttaccgtc
     1201 ggcggctgcg gcggctgacg cgcatggtcg ggaaaagtgg gagacggttt tccgacagga
     1261 tcatgcgcaa atcgtaggtc agggagcaat aaatggcatc gacaatccgc gtgcgcgcga
     1321 tttcaaatgg cgaagtcacc gaggtgcagg ccctgatcca gcacccgatg gatacgggcc
     1381 tcgtaaagga tgcgaagggc gacctgattc cggcgcattt catccagcag ctcaccttcg
     1441 agcataacgg caggacggtg tttttggccg attggggtcc ggctgtcgcc agagacccct
     1501 atgtgaagtt cagcttcaag ggagcggcca agggcgacga cctcaagatc gcctgggtcg
     1561 acaacaccgg cgcaaccgac acgatgatgg cgaagatcca gtgacgatgc gcgtgcgttt
     1621 gcttctgcag tctgggttgg ccgcacttgc gttggcgatt tttattcagc cggcaaccgc
     1681 cgccgacacg cccgatcctg ccgccgacgc caaggcgttt cgcgattact tcgtgaaaaa
     1741 attccccaag gtgaagctcg aggatttcgt caacggtccc tattcgctga acgaggatat
     1801 gcgccggcag tgggaggaga aggagcagtt cccgccttac gagttcgcgc tcgaagccgg
     1861 caaggaaatg tttgcgacgc cattcaggaa cggcaagacc tacgcggatt gctttccgaa
     1921 cggcggcatc ggcattcgcc agaactaccc ctatttcgac gagaaggaag gcaaggtcgt
     1981 cacgctggaa ctcgcgctca atcgctgccg cgaggccaac ggcgaagcgc cttattccta
     2041 tgtgaaggac gagatggctt cgctgaccgc ctacatggcg ttcacctcgc gcggcaagcc
     2101 gatggacatc aaggtcccga acgatccgcg cgcgctcgcc gcttacgagg acggcaagcg
     2161 ctatttctat acgcggcgcg gccagctcaa tttctcctgc gcgacctgcc atgtgcagag
     2221 cccgggcgag cgcatccgcg ccgagatcct ggcgccggct tacggcatcc tgaacgcgat
     2281 gccgatctat cgctcggaat ggaacggcat gggcacgatc agccgtcgtt tcgtcacctg
     2341 caacagccag acccgtgcgg ttccactcga gccgcaatcc gatgaatatc gcgacgtcga
     2401 atatttcctg tcctatgtgg cgaacggatt gccgatcgca ggtcccggag cgcggccatg
     2461 agcatgatgc gaaatacgct gctggctatg gtgttgctga tgaccggcgc catgccggct
     2521 ctggccgcct cggaggccga ttacaaggta gcattggccg cggctgaggc ggcggaaaag
     2581 caggc
//
LOCUS       DATLJT010000617         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1874869,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000617 DATLJT010000000
VERSION     DATLJT010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874869"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            579..929
                     /locus_tag="VM910_14295"
     CDS             579..929
                     /locus_tag="VM910_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63736.1"
                     /translation="MPAPNDRTDRMLDLARRWRESGQSARAFAQEQDVTPWTLYYWRE
                     RLAGEKPRPARRRKPRSSARVALAPVHVVTSGNEESRSDVEILLASGDRVRVTASVSV
                     ETLRRVVQILRAPC"
     gene            923..1273
                     /gene="tnpB"
                     /locus_tag="VM910_14300"
     CDS             923..1273
                     /gene="tnpB"
                     /locus_tag="VM910_14300"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HVI63737.1"
                     /translation="MLTISPAVRIYLATGTTDLRRSIDGLAALVRERFELDPTSGHLF
                     LFRNRRGDRVKVLVWDAGGFCLLYKRLERGTFAWPAGEDEARVEMHSRDLLLLLSGVD
                     LAHTRRRRWYERVA"
     gene            1325..2860
                     /locus_tag="VM910_14305"
     CDS             1325..2860
                     /locus_tag="VM910_14305"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI63738.1"
                     /translation="MDQSRAIEDRIDDLPTARALIASLQDQLTKSQREIAALRHELDI
                     LCQRLFGKKSERVDPRQLQLALEQLGNERGSITEPVEMDSGETPVRGHTRRRPTGRRP
                     LPAHLPRRRVEIDLSDAEKQCPCGQTRTRIGEDSSEKLEYEPASFVVIETVRPKYACP
                     GCHAGVVEAPSPPQAVEKSLAGEGLLAHVVVSKYVDHLPLHRLEGVFARERIDLPRST
                     LCGWVADVATALTPIGEQLRREITAADYLQTDDTSITVLDERGGSFKGRLWTYLDPLA
                     RQVVFDATPTHEGDGPAAFLAPFQGALQADAYSGYDALYQSGRVIEIGCWAHVRRGFV
                     EAFMIDTAAALMIALIQQLYQVEQAAADLDPDGRRALRQEQSTPLLTKIDTERQALAR
                     TVLPKSPLGEAVRYLTNQWAALQRFVDDGRLAIDNNRAENMLRIVAVGRKNWLFAGSF
                     EGARRAALLYSIVQSCKLVDLAPFPYLKDVLLRLATHPHRLIDQLTPRRWAAIFGLQP
                     PPI"
BASE COUNT          568 a         1018 c          983 g          573 t
ORIGIN      
        1 catcagctcc tcgcggagtt ggtcaagcaa catgtccacg ccatctactt cgatggcttc
       61 gaacgtcacc ccatcgaccg cagcgagtag cagagcggtc ccaacagctt ccgacacgag
      121 ccgtcgagac gatgaacgca cgatgtccga tgtcatgatg tccgttcgat ccgcggcgtc
      181 ccgtgacgct gcttgtcttt cattcgcgta cacgaatatc acggcacaaa aaaactcagg
      241 cggggcagtc tccgccgcgg gcgcgcacct gccgagcgcg ctctctatcc cccgccagtt
      301 cgggcacatc gcgaaaacaa gtccccaaac actccaacag cttcttgtgg aagggcgatc
      361 gactcggcgc gagggcgtag aaattccagg accgctcctg ccgcacgtcg accaacccgg
      421 ccttgcgcag atacgacaga tgccgtgaga cggtgggctg cggcaggcgc agaatctcga
      481 ctagatcgca cacacacagt tcgcctgtaa gggttcgatg aaccccgtct ttcgttgatc
      541 gtcccttccg agtgatgctg gtgccggagg agatccggat gccggcaccg aacgaccgaa
      601 cagatcgcat gctggacctg gcccgccgct ggcgagagag tggccagagc gcccgcgcct
      661 ttgcccagga gcaggacgtg acaccctgga cgttgtatta ctggcgggag cgcctggccg
      721 gtgaaaagcc gcgaccggcg aggcgacgga agccgcgatc gtcggcacgg gtggcactcg
      781 cgccggtgca tgtcgtgacg agcggcaacg aggagagccg aagcgacgtg gagatcctgt
      841 tggcgagcgg cgatcgggtg cgcgtcacgg cgagcgtatc ggtcgagacc ctgcgtcgcg
      901 tggtccaaat cctgcgcgcg ccatgctgac gatctctccc gcggttcgga tctatctggc
      961 caccggcaca accgatctgc ggcgatcaat cgacggactc gcggcgctgg tgcgcgagcg
     1021 cttcgaactc gacccgacct cgggccatct gttcttgttt cggaaccgtc gaggcgatcg
     1081 cgtgaaggtg ttggtctggg atgccggtgg gttttgcctc ctctacaaac ggcttgagcg
     1141 tggcacgttc gcctggcccg ccggcgaaga tgaggcgcgg gtcgagatgc acagtcgcga
     1201 tctcttgctc ttgttatcgg gtgtcgatct cgcgcacact cgacgtcggc gctggtacga
     1261 acgcgttgcg tgaggcggca cgcgacggta aacatcttca gccgctgatc gtatagagag
     1321 gcagatggat cagtcgcgag cgatcgagga ccgcatcgac gatcttccca ccgcccgtgc
     1381 cctcattgcc tcgttgcagg accaactgac gaagtcgcaa cgcgagattg ccgcgttgcg
     1441 ccatgaactc gacatcctgt gtcagcggct gttcgggaag aagtccgaac gcgtcgatcc
     1501 gcggcaactc cagctcgccc tggaacaact cggcaatgaa cggggatcga tcactgaacc
     1561 cgtggaaatg gattcggggg agacgccggt gcgaggccac acgcggcgcc gccccacggg
     1621 gcggcggccg ctgcccgcgc atctgccgcg gcggcgggtc gagatcgacc tgtccgacgc
     1681 ggagaagcag tgcccgtgcg gccagacgcg gacgcgcatc ggcgaagaca gttccgaaaa
     1741 actcgaatac gaacccgcga gtttcgtcgt catcgaaacg gtccgcccga aatatgcgtg
     1801 tccgggctgt cacgcggggg tcgtcgaagc cccgtccccg ccgcaggccg tggagaaatc
     1861 tctcgccggc gaaggtctcc tcgcccatgt cgtcgtctcg aagtatgtgg accacctccc
     1921 cctgcaccgc ctcgaaggcg tcttcgcgcg ggagcgcatc gatctgccgc gcagtacctt
     1981 gtgtggctgg gtcgcggacg tggcgaccgc gctgacgccc attggcgagc agttgcggcg
     2041 ggagatcacg gcggccgact atctccagac cgatgacacg tcgatcaccg tccttgacga
     2101 gcggggcggc agcttcaagg gtcgcctctg gacctatctc gatccgctcg cgcgccaggt
     2161 cgtcttcgac gcgacgccca cgcatgaggg cgacggcccc gcggccttcc tcgctccctt
     2221 tcagggcgcg ctgcaagcgg atgcttatag cggctacgac gcgctctatc agagcgggcg
     2281 cgtcatcgag attggctgct gggctcacgt acgccgtggc ttcgttgagg ccttcatgat
     2341 cgacacggcg gcggcgttga tgatcgcgtt gattcaacag ctatatcaag tcgagcaggc
     2401 cgccgccgat ctcgatccgg acggccggcg cgccctgcga caggaacagt cgacgccatt
     2461 gctcacgaag atcgataccg agcggcaggc cttggcgcga accgtgttgc cgaaatcgcc
     2521 actcggtgaa gccgttcggt atctgaccaa ccagtgggcg gcgctccagc gcttcgttga
     2581 cgatggccgg ctggccatcg acaacaatcg tgccgagaac atgctccgca tcgtggcggt
     2641 tggtcggaag aactggctct tcgcaggcag tttcgaaggg gctcgccgtg ccgcgctcct
     2701 ctattccatt gtccagagct gcaaactcgt tgatctggcg cccttcccgt acctgaagga
     2761 tgtcctgctc cgtctggcga cgcacccgca tcggctcatc gatcaactga cgcccagacg
     2821 ctgggccgcg atcttcggcc tgcagccgcc gcccatctga tccgactcca ttcgattctg
     2881 aacaatggtc agtggtcgcg gctgcgtgta acttgtcaga cggtctttgt cgaacggtta
     2941 cggtgtcgac acagacggcg aaccgacgac tcggcgcgac ggcctggcgc gacttaatcg
     3001 aggtacctct tgcgcttgaa ttcctttttg ccgatgccgt gtgcttcata ccaatgcagg
     3061 tccgcgagcc gaaccacgcc gctcctgagt cgaaatccgc gcgacgcctt tcatcttcct
     3121 ccatccaccc gtgccataca ag
//
LOCUS       DATLJT010000618         4935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3713097,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000618 DATLJT010000000
VERSION     DATLJT010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3713097"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..356)
                     /locus_tag="VM910_14310"
     CDS             complement(6..356)
                     /locus_tag="VM910_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63739.1"
                     /translation="MLVAVFLLMGLAAQAQEQPKQAQEQPKYEVSGFRDARFGMTEAE
                     VRAAVKKSFAVRGAAPRRRHQVSGESRRERDDVPRSEEGAEARHQLHRGSRESRYCEN
                     RTGEILTINPAHRF"
     gene            1192..2922
                     /locus_tag="VM910_14315"
     CDS             1192..2922
                     /locus_tag="VM910_14315"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HVI63740.1"
                     /translation="MQWLRRILLWALFVAGAGGLAHAQVLAPAPSQVTPPRLPAAPRV
                     APEIPSVQPPEAPAFPKDAEKLSFVLLGFDVGGEFEEFAATRRELAAPLIGKRVTVAQ
                     VFDFATQLQAAYVRAGYLLARVVVAPQELNERARVKVNVVDGFIERIDVSALPPAIGN
                     RVAAVLEPLFHKHHLTESELERRLLLASDTPGAQLRSTFAAGEEIGGSVLIVTGSYRP
                     VVLSGYTDNAMPVTFGTLQSVALLGLNSALGMGESITVSTTGFPSHDFATEFPTRRFL
                     QGSLVFPVGIDGLKFLIAGTDGRTTPNVDLLAQTQGIFRQNTYVLAYDAVKRRDFQLT
                     LSGRFDATYERIDTLVLTPPVSLSLDQVRPVRGSVDGNWTLRETNTNILFGATVSRGL
                     NALGARTLADATPELPLSRQGADAVFTKLNAALAITQSLPSDFFIATTSYAQNSFHKP
                     LLTSEQFDIIGPKMISGLPVGLLPGDSSWVVPTELGHVNQFPIESGGLTITQYIFGAT
                     GERIFYQPTAVELASVHVTNYGVGLRFVTTRWAELMPNSYAFVEWSQLRSSDPTLDRW
                     RFFSGVLIQY"
     gene            3431..4807
                     /locus_tag="VM910_14320"
     CDS             3431..4807
                     /locus_tag="VM910_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVI63741.1"
                     /translation="MATTKVLIRAPQAPERLAAARMTGRYLVTIEPDAHREISARLNK
                     VGIKSAPPLPRVAASAKPLADGSHLTLRKVGVALVDPKPEQEDALHRFAAQERAVIAL
                     EPERIVRAVEVDRAGDYLRGWRDAVDALAGKLLEEQRPPAPVPSEAAVPGATWGLAAT
                     KVTSTQLSGANIKVAILDTGFDLSHPDFAQRQIVTKNFVGDQQPFHDGVGHGTHCTGT
                     ATGPLHPASVQRYGIAFEALIFSGRVLDDTGSGGDFNILQGIDWAIEQGCDIVSLSLG
                     APWVPGDPAFSPAYESAAQHALAAGCLLVVAAGNEANDPRFVGAVGTPGNSPSVLTVA
                     AIDRNLATAPFSNRVQPDAPGVKGPDLAAPGVDVFSSWLVREGRYNTISGTSMATPHV
                     AGIAALFAQANPSIRGQALKDMILNHCMALPNGAARKGEIGRGLVQAPLDSQSVSRTD
                     RRRRRRVG"
BASE COUNT          972 a         1470 c         1422 g         1071 t
ORIGIN      
        1 cgtcatcaaa atcggtgcgc cgggttgatc gttagaattt ccccggttcg attttcgcaa
       61 tatctggatt ctcgcgatcc ccgatgtaat tgatgacgag cttcggcgcc ctcttcggat
      121 cgggggacgt cgtctcgttc ccttcgcgat tcacctgata cttgatgccg tcgacgagga
      181 gccgcaccgc ggacggcgaa agacttttta acggcagcgc gaacttccgc ttcggtcatc
      241 ccgaagcgcg catcacggaa cccgctcact tcatacttag gctgctcttg cgcttgctta
      301 ggctgctctt gcgcttgcgc agccaaaccc atcaaaagaa aaacggcgac gagcatgact
      361 cgccgccacg cgcgagcgcc gcagcacatg tcctacactc ctcaaccgag cggtgctgac
      421 tggcggccgc tccctctgga tttactcagc ttatttttga attcacgggg gctgtttttg
      481 agtcggctct tttataggtc cggttcaatt ttcaccggac attagttctt cccgagcatt
      541 tttctcgtcc ttactgcttc ggattccacc ccgtggtgag gctggtatac cagcgggtta
      601 actgtttgtt aagcccaatc tacaagcggt taaatcggat gagggccgtc cttcgcgaca
      661 aagcgccgca tgaggtgggc gcgtgtttgg atcggtttct gttccaggct gcagtacacg
      721 gaatggttat gccagcaaca gaacacaact ctttcccatg atgcgctttt cccatgatgc
      781 gcttgaggca ggggatgccc ggggctgacc attgctatcc cgtcgaaaac tggcacgcgc
      841 gttcttaaag ctttgcatgt ggcgagcgtg gagggcgctc ggcgcctcat gagtcgccct
      901 gcgcgacatc gcggctcttc cgtcacgcga ttgaacgcag gccgcgtcga gtcccctgtg
      961 cctattttgc cactctcaag agaattacgg tgccgctcga acctgctggc cctggaccgc
     1021 atccgaagcg atttatcatt ggcgctgatt cggtgtgcaa tcgcggcaca accgatcctt
     1081 tttgagcagt gtgtcgttta aacgcgtttt tgcgtcggtc tgtatcccaa gttgcagagt
     1141 tcgcaagcaa gccctcgcaa gggccgggcc ggttctatgc gcgcaagcag catgcaatgg
     1201 ttgcgacgaa tactactgtg ggcgttgttt gtcgcgggag ctggcggtct agctcacgcg
     1261 caggtgctag cgcctgcgcc gagccaagtt acaccgcctc gtcttccggc tgcgccgcga
     1321 gttgcccctg agattccttc ggtccagccc cccgaagccc ctgcttttcc gaaagatgcg
     1381 gaaaagctca gcttcgtgct cctcggtttc gatgtcggag gcgagtttga ggagtttgcc
     1441 gccaccaggc gcgaactcgc cgcgccgctc attggcaagc gggtgaccgt cgcccaagtc
     1501 ttcgatttcg ccacccagct gcaagcggcg tatgtccgcg ccgggtatct tctcgcgcgc
     1561 gtcgtcgtgg cgccgcagga attgaatgaa cgggcacgcg tcaaggttaa tgttgtcgat
     1621 ggcttcatag agcgaattga cgtcagcgcc ttgcccccgg cgataggaaa tcgcgtcgcc
     1681 gccgtgctcg agccattatt ccataagcat cacctgaccg agagcgagct cgagcgccgc
     1741 ttgttgctcg cgtcggacac gcccggcgcg cagcttcgct cgacatttgc cgcgggagaa
     1801 gaaattggcg gctccgttct tatcgtgaca ggttcgtatc gccccgtcgt actcagcggc
     1861 tataccgaca acgcgatgcc ggtgacgttc ggcaccttgc agagcgtcgc ccttcttggc
     1921 ctcaacagcg ctctcggcat gggtgagtcg atcacggtat cgaccaccgg ctttcccagc
     1981 cacgattttg cgaccgagtt tccgacacgg cgctttctgc agggttccct ggtctttccc
     2041 gtgggaatcg acggattgaa atttttgatt gcaggcaccg acgggcgaac gaccccaaac
     2101 gtcgatctat tggcgcaaac ccagggcatt tttaggcaga atacgtatgt ccttgcctac
     2161 gacgccgtga agcgtcgtga tttccagttg acgctcagcg ggcgatttga cgccacttat
     2221 gagcgcatcg acacgctcgt gctgacgccg ccggtttcgc tcagccttga ccaggtgcgg
     2281 ccggtcagag ggtccgtgga cggcaactgg acgcttcggg aaaccaacac caacatcctc
     2341 ttcggcgcca ctgtttcccg gggcctcaat gcacttggag cgcggacctt ggcagacgct
     2401 actcccgagc tgccgctgtc gcggcaggga gccgacgccg tcttcaccaa gctcaacgca
     2461 gcattagcta tcacgcagag tctcccgtcg gatttcttca ttgccacgac gagctatgca
     2521 caaaactcgt tccacaagcc gctgctcacg tcggagcagt tcgacattat cggtccaaaa
     2581 atgatatccg gcctgcctgt cggcctgttg cccggcgact cgtcgtgggt cgtgcctacc
     2641 gagctaggac atgtgaatca gtttccgatc gaatccggcg gactcaccat cacgcaatat
     2701 atattcgggg cgacgggcga gcgcatattt tatcagccta ccgcggtcga gctcgcaagc
     2761 gtgcatgtca cgaattatgg cgtagggctg cgtttcgtta cgacgcgttg ggccgagctc
     2821 atgcctaaca gttatgcgtt cgtggagtgg agccaactgc gaagttcgga tccgacgctc
     2881 gaccgttggc gtttctttag tggggtgctc attcaatact gagtggcttc ttcgcctgcg
     2941 cattccgacg taaagcgcgc cgacgcgccg gcaaattgag cttccgaaca tctggatgga
     3001 ttggcggaga gtgtccgcct ctagcttcat gaaatcaaag gcttagcggg cgctccatac
     3061 gtttcccata aattatccca caagccaatt ggccccaaac gcacgccggc cttgagggca
     3121 agaaatcctg tccatgaccg ccgcgggacg tgtccgatgt ctgctttacc cccgaaagcg
     3181 gacactgagc gacacgactc gcatgtccgc tttgtgccaa aagcggactc atgcactgca
     3241 gcaaatagct tcgctattcg atcacctcgt cggctcgggc aagcacagtc gcccgtaagt
     3301 gtgaactact tcattctttt gcagcaaagg gttcacgtac gactattgat tgagaacaat
     3361 cgccgcggcg gggcaattgt gcctttgact cgacgatgta gtcgtcgatc ctgagagaga
     3421 ggtactggca atggcaacaa ccaaagtgtt gatccgcgca ccgcaagctc ccgagcgtct
     3481 tgccgcagcc cgtatgacag gacgatatct tgtgactatc gagccggacg cgcaccggga
     3541 aatatcagca aggctgaaca aagtcgggat caagtcagcc cctccgctcc cgcgggtcgc
     3601 cgcgtcagcg aagcccctgg ctgatggaag ccatctaact ctgcgaaagg tcggcgttgc
     3661 cctcgtcgat ccgaaacctg aacaggagga cgcgcttcat cgcttcgcag cacaggagcg
     3721 cgccgttatt gccttggagc ctgaacgtat cgtccgggcg gtcgaagtcg atcgcgctgg
     3781 cgattattta cgcggctggc gcgacgcagt ggacgcgctt gcgggaaagc tgctcgagga
     3841 acagcgtccc ccagctcccg ttccgagcga ggcggcggta ccgggcgcca cctgggggct
     3901 agccgcgaca aaagtcacaa gcacacagtt atcgggagcc aacatcaagg tggccatcct
     3961 cgacaccggt ttcgatctga gccatcctga ctttgcacaa agacaaatcg taactaagaa
     4021 cttcgtcgga gatcagcagc cattccacga tggcgtagga catggcaccc actgcaccgg
     4081 cacagccact ggcccactgc accccgcaag cgtgcaacgt tacggcatcg catttgaggc
     4141 tttgatcttc tccgggcggg tactcgacga tactggcagc ggtggggact tcaacatcct
     4201 gcaaggaatt gactgggcga tcgagcaggg ttgcgacatc gtttccttgt cgcttggagc
     4261 cccttgggtg cccggagatc cggcatttag cccagcttac gaatcagcgg cccagcacgc
     4321 gcttgccgcg ggctgcctgt tggtcgtcgc tgccggaaac gaagccaatg atccgcgatt
     4381 tgtcggcgcg gtgggaacac ccggtaattc cccctcagta ctgacggtcg ccgcgataga
     4441 tcgcaatttg gccacagctc ccttttccaa tcgagtgcaa ccggatgcgc ccggggtaaa
     4501 aggaccagat ctcgcggcgc ccggtgtcga cgtgttttcg tcatggctgg tgagggaggg
     4561 tcgctacaac accatttccg gaaccagcat ggccacaccg cacgttgctg ggattgcggc
     4621 gctgttcgcg caggccaatc caagcattcg tggtcaagcg ctcaaggaca tgatactgaa
     4681 ccactgcatg gctctgccaa acggcgcggc gcgcaagggt gagatcgggc gcggattagt
     4741 gcaggcacct cttgattcgc aaagcgtttc gaggactgat cgacgccggc ggcgcagggt
     4801 tggatgagcc aataaatgag gcgacatgac cgaaattcca gtaatcgtgt tggtggacgg
     4861 tggcgcggat ctgcaaaaga tcaaagccaa ttgtgagagg tatggattga cacaggtgcg
     4921 cgcactaccg cgatt
//
LOCUS       DATLJT010000619         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_267322,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000619 DATLJT010000000
VERSION     DATLJT010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267322"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VM910_14325"
     CDS             <1..1450
                     /locus_tag="VM910_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI63742.1"
                     /translation="GTVTHVDDSATKKLPGVRQVVVLDDLVAIVGDHMWAAWQGLDAL
                     NVTWDEGPNAKINSADIWNALRAASKKDGFVAKSEGDVDKGLTQGERFDADYEVPLLA
                     HAVMEPMNCTVRLTPGTCEIWTGTQVQSRCQEYAARAAGIPVDKVTVYNHYLGGGFGR
                     RLEPDMVESAVRIAKQVSTPIKVVWTREEDMRHDVYRPAYRDTISASLSNGKIVAWKY
                     KVSGSSVLARWLPPAFQNGIDGDAVDSAVDMPYDIPNNRIEYVRVEPPAVTTGFWRGV
                     GPNNNVFAIESFIDELARRVNMDPVAFRLGMLDGTPRLQAALKLVAEKSGWSSPLPAR
                     VGRGVSAQPSFGSFIATVVEAEVDDVGAVHLRRITCAVDTGIAVNPDTIIAQLQGGLI
                     FGLTAALWGEVTIEQGRVQQSNFNDYRMLRINEIPPIDVHVIKSTEHPGGIGETGATA
                     GPPALRNAIYAATGVPLRRLPIDGAAIALKKA"
     gene            1456..>2267
                     /locus_tag="VM910_14330"
     CDS             1456..>2267
                     /locus_tag="VM910_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI63743.1"
                     /translation="MRRSAIIVIAAIIIVAVGVFAWFILTRGPLAFAGGSTVALSDYH
                     EANPTGVPAELAQVDIVRRGEYLAKAADCVVCHTAPGGASYAGGLAFPLPFGSLYSTN
                     ITADKNTGIGNYSDQDFLNAVQRGIRKDGVRLYPAMPYTSYTLLTDADVLAIKAYLFS
                     LPAQHRQNQRDTLLFPFNQRWSMIFWSWAFNADTRFAPNTAKSAEWNRGAYIAEALAH
                     CGECHTPRNLAFALNNWRKFAGAVAAGWRANDITSDKGTGIGAWSDGEVFDY"
BASE COUNT          436 a          719 c          694 g          418 t
ORIGIN      
        1 cggcacggtg acgcatgtcg acgacagcgc cacgaaaaaa ctgcccggtg tgcggcaggt
       61 cgtcgtgctc gacgatctcg tcgccattgt cggcgatcat atgtgggccg cctggcaggg
      121 actcgacgcg ctcaacgtga cttgggacga gggaccgaac gcaaaaatca actcggccga
      181 catctggaat gcgttgcgtg cggcgagcaa gaaggacggt ttcgtcgcca aatccgaagg
      241 cgacgtcgac aaaggcctga cgcagggcga acgcttcgat gccgactacg aggtgccgct
      301 cctcgctcat gccgtgatgg agccgatgaa ctgtacggtg cgccttacgc cgggcacctg
      361 cgagatctgg accggcacgc aggtgcagag cagatgccag gaatatgcgg ccagggcagc
      421 gggcatccct gtcgacaagg tgacggtcta caaccattac ctcggcggcg gcttcggccg
      481 ccggctcgag cccgacatgg tcgaaagcgc ggtgcgcatc gccaaacagg tctcgacccc
      541 gatcaaggtc gtgtggacgc gtgaagagga tatgcggcac gacgtctacc ggccggccta
      601 tcgcgacacg atttccgcct ccttgtcgaa cggcaagatc gttgcctgga aatacaaggt
      661 atcgggctcg tcggtgctcg cgcgttggct gccgcctgcg ttccagaacg gcatcgacgg
      721 tgacgccgtc gatagcgctg tggacatgcc ctacgatatc ccgaacaacc gcatcgaata
      781 tgtacgtgtc gagccgcccg cggtgacgac cggcttctgg cgcggcgtcg ggccgaacaa
      841 caatgtcttc gctatcgaaa gtttcatcga cgagctcgcg cgcagagtga acatggatcc
      901 ggtcgccttc cgcctcggca tgctcgatgg cacgccgcgg ttgcaggctg cgctcaaact
      961 ggtcgccgaa aaatccggct ggagctcgcc cctgccggcc cgggtcgggc gcggcgtttc
     1021 cgcccaaccg tccttcggca gcttcatcgc taccgtggtt gaagccgaag tcgatgatgt
     1081 cggcgccgtg catttgcgac gcatcacgtg cgcggtcgat accggcatcg ccgtcaatcc
     1141 cgacactata atagcgcaac tccagggcgg gctgatcttt ggcctgaccg cggcgctatg
     1201 gggcgaggtc acgatcgagc aaggccgcgt gcagcagtcg aactttaacg attaccgaat
     1261 gctgcgcatc aatgagatac cgccaatcga cgtccatgtg atcaaaagta ctgagcatcc
     1321 cggaggcatt ggcgagacag gtgcaaccgc cggcccgccc gccctgcgca acgcgatcta
     1381 tgcggcgacc ggcgtgcccc tgcgccgtct gccgatcgac ggtgccgcaa ttgcattgaa
     1441 gaaggcgtaa tcgccatgcg ccgcagcgcc attatcgtca ttgccgctat catcatcgtt
     1501 gccgtcgggg tcttcgcgtg gttcatcctc acccgcgggc cgctcgcgtt tgctggcggc
     1561 tcgacggtcg cactctctga ctatcacgag gcgaatccga ccggtgttcc ggcggaactc
     1621 gcccaagtcg acattgtccg gcgcggcgaa tatctcgcca aggctgccga ctgcgtggtt
     1681 tgtcacaccg cgcccggcgg agcgagctat gctgggggcc tcgctttccc gctgccgttc
     1741 ggcagcctct attcgactaa catcaccgcc gacaaaaaca ccggaatagg caattacagc
     1801 gatcaggatt tcctcaacgc ggtgcagcgc ggcatccgca aggatggcgt gcggctttat
     1861 ccggcaatgc cctacacatc atatacgttg ctcaccgacg ccgatgtgct ggcgatcaag
     1921 gcctatcttt tcagcctgcc ggcccagcat cggcagaacc agcgcgatac gctactattc
     1981 ccctttaacc agcgctggtc gatgatcttt tggtcatggg cattcaacgc cgacacgcgc
     2041 tttgcgccga acaccgcgaa gagcgccgag tggaatcgtg gagcctatat cgccgaagcg
     2101 ctagcgcact gcggcgagtg ccacacccct cgcaatctcg catttgcgct gaacaactgg
     2161 cggaaattcg ccggcgccgt cgccgccggt tggcgtgcca atgacattac atctgacaag
     2221 ggcacgggca tcggcgcgtg gagcgacggg gaggttttcg actattt
//
LOCUS       DATLJT010000620         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3942520,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000620 DATLJT010000000
VERSION     DATLJT010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3942520"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1418
                     /locus_tag="VM910_14335"
     CDS             69..1418
                     /locus_tag="VM910_14335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVI63744.1"
                     /translation="MMIVGAAWTQTEARAPRKDPPKRPIHGPNYHPPYAAIVVDDKSG
                     FVLHEVSADEPRHPASLTKIMTLYLLFEQLEAGKLSLDTELPISTWAALQNPTKLGLK
                     ANQTIKVEDAIKGLVTKSANDAAVVVAEAIGGSEAEFAKLMTLKARMLGMTSTTYVNA
                     SGLPAEEQITTARDQAVLGRAIHHRFPVYYQYFATPSFRYKGAEMRNHNNLLGQVKGV
                     DGIKTGYTEASGYNLVSSVRRDEKHIVAVVLGGTSNGARDARMRQLIEDNISLASTQR
                     TVPVIVDANGWRIADEGWALYLAAQWLEDARWRYQSPSRIAGFYGDLGWKRTDAEIRL
                     VASEANNFFGVSEPTRAQLRAANGRYSYRRIVQSNVYGRALGQGNPLFNAPCVENDGV
                     PTQPQANVQILGLINQPTKYSPGSGNACAPTSWGAVERTFINALTTSGESQTVTTTR"
     gene            1559..2326
                     /locus_tag="VM910_14340"
     CDS             1559..2326
                     /locus_tag="VM910_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63745.1"
                     /translation="MLGNLSLLSTIGLAICLNASADAACIDLKQTDTLSFEGTLNYRI
                     FAGPPNYEDVRKGDTPEPTYILKLDDPICATGDEFVDQNVKFDRIQIFPESGKAGQAL
                     WRDLRSLIGQRVVVAGSGAFGRHTGHHHAPLMLPIASISAAFDLTKSHGTSMTTVQGF
                     YLALSAGNGEEAVKFVVPEKRSSGPLSAIAIRNFYSNLVEPLTLIDVVPTRPDEYRVR
                     YTYVAPSRGRCDGEALVRTTKLNGLNLIDSIKAISGC"
     gene            complement(2586..3149)
                     /locus_tag="VM910_14345"
     CDS             complement(2586..3149)
                     /locus_tag="VM910_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63746.1"
                     /translation="MIMAWLLRKWGLGKDKTAATGGHDPAIRRPDFETMFVFWTGLLA
                     ERGLPQAVRWVFHEDFARVPSARDWRFSFRLRPAMEADRIARFAYAHLDPKKPLAIVA
                     YAVDDGSVVTGFQGDVFSANEDVYRDDWNIYFDARETKGMISSDCQFVFDDVTWARMR
                     AEQPRYLSELDYLVSVDALRRQFGYRD"
BASE COUNT          727 a         1016 c          999 g          674 t
ORIGIN      
        1 ctcacatcgc gccggcgagg atatggaccg cctcccattg gctgcgagtt gccggtgtgc
       61 tgataacgat gatgattgtc ggcgcggcgt ggacgcagac ggaagctcgt gctccccgca
      121 aggatccccc gaagcgccca attcacggac ccaattatca tccgccgtac gcggccatcg
      181 tggtcgacga caagtcgggc ttcgtgctgc acgaggtcag tgccgatgaa ccgcgccacc
      241 cagcgtcgct gaccaagatc atgacgctct atctgctgtt cgagcagctc gaggccggca
      301 agctcagcct cgacacggag ttgccgatct cgacgtgggc ggcgctgcag aatcccacga
      361 agctcggcct caaggccaat cagaccatca aggtcgagga cgcgatcaag gggctcgtga
      421 ccaaatcggc gaacgacgcc gcggtcgtcg tcgccgaagc catcggcggt agcgaagcgg
      481 aattcgccaa gctcatgacc ttgaaggcaa ggatgctggg catgacgagc acgacctatg
      541 tcaatgcctc agggttgccg gccgaagagc aaatcacgac cgcgcgcgac caggccgttc
      601 tgggccgtgc catccaccac cgtttcccgg tctactatca atattttgcc actccgagct
      661 tccgctataa gggcgccgag atgcgcaacc acaacaacct gctcggacag gtgaagggcg
      721 tcgacgggat caagaccggt tataccgagg cttccggata caacttggta tcatcggtgc
      781 gccgtgacga gaaacacatc gtcgcggtgg tgctcggcgg gacctcgaat ggcgcgcgcg
      841 atgctcgcat gcgccaattg atcgaagaca atatttcgct ggcttcgacc cagcggacgg
      901 tgccggtcat cgtggacgcg aacggctggc gcatcgcgga cgagggatgg gcgctctatc
      961 ttgccgccca gtggctcgag gatgcgcgct ggcgctacca gtcgccgtcg cggatcgccg
     1021 gcttctacgg cgatctcggt tggaagcgca ccgatgcgga gatccgtctg gtggcgagcg
     1081 aggctaacaa tttcttcggc gtgagcgagc cgaccagggc gcagttgcgc gccgcaaacg
     1141 gccgctactc ctaccgccgg atcgttcaga gcaatgtcta tgggcgcgcg cttgggcaag
     1201 gtaacccgct attcaatgcg ccgtgtgtgg agaacgacgg ggtgcccact caaccgcagg
     1261 cgaatgtcca aattctcggc ctcatcaacc agccgaccaa atattccccc ggcagcggca
     1321 acgcttgcgc gcccacgtcc tggggcgcgg tcgagcgcac cttcatcaac gcgctcacga
     1381 ccagcggaga gtcgcagacg gttacgacga cgcggtgacc ggcggacgat ccgagccgcg
     1441 cggagaattc cgcggcgcag cggtcttctc ccgccgtatc atctgatatc cttctgctcc
     1501 gatgtgcggg gggcgaccgc aaacgcacct ccattcagaa cgattctgac tgactatcat
     1561 gcttggaaac ctttcgcttc tttcaacgat cggcttggcc atctgcctca acgcgtcggc
     1621 ggacgccgca tgcatcgact tgaaacaaac ggataccctc tcgtttgagg gcacattgaa
     1681 ttaccgcatc tttgcaggac cgcccaacta cgaggatgtt cgaaagggcg atacgcctga
     1741 acctacgtac atcctcaagc tggacgaccc aatttgtgct accggagatg aatttgtcga
     1801 tcagaacgtc aagttcgacc gaattcaaat atttcctgag tccggcaaag ccggtcaggc
     1861 gctatggaga gacctgcgga gccttattgg gcaacgcgtc gtcgttgcag ggagcggagc
     1921 attcgggcgc catacggggc accaccatgc tccgctgatg ttgcccatag ccagcatttc
     1981 agctgctttc gatctgacaa agtctcacgg cacatcgatg accacggttc aaggcttcta
     2041 cttagccctt agcgcgggca acggtgagga agcggtaaaa tttgtggttc ctgaaaagcg
     2101 atcgtcggga ccactatccg caatcgctat cagaaatttc tacagcaatc tggttgaacc
     2161 gctgactctt attgacgtgg ttcccacgcg tccggacgag tatcgcgtac gctatacata
     2221 tgttgcacca agtcgcggac gctgtgatgg agaggcgctt gtccggacga ccaaactcaa
     2281 cggacttaac ttgattgatt caatcaaagc gattagcgga tgctaggaaa ttgttttacg
     2341 atagcgtttc atggacgttc gcgactgttc ggcatgaggc ggcgggagtt cattgcactc
     2401 ccgcgaattc cggctgcgcg ctgacgaggt gatcgaatga gacgccgcga gttcatcatc
     2461 attctcagga tcgcacggct tgcgggcagg ccgcgcccgg gcgcaagtta gtccagaagt
     2521 cgaatatggt ttcgaaagca gggctgcgtt tgcaagccaa tacgctctgc tcgggaagcg
     2581 gtcagctaat cccgatagcc gaactgacgt cgcaaggcat caacagatac gaggtagtcg
     2641 agctctgata gataacgcgg ttgctccgca cgcatgcgcg cccacgtcac atcatcgaat
     2701 acgaactggc aatcgctgga aatcatgccc ttcgtttccc tcgcgtcgaa atagatgttc
     2761 cagtcgtcgc ggtacacatc ctcattggcg ctgaagacgt cgccttgaaa tccggtgacg
     2821 accgagccgt cgtccaccgc ataggccacg attgcaagtg gcttcttcgg atcgaggtgc
     2881 gcgtaagcga agcgagcgat ccggtcggcc tccatggcag gccgcaggcg aaatgaaaac
     2941 cgccagtcgc gggccgaagg tacgcgcgca aaatcctcat gaaacaccca tcgcacggct
     3001 tgcggcaggc cgcgctcggc gagcaagcca gtccagaaca caaacatggt ttcgaaatcg
     3061 gggcgtcgga tggctggatc gtgcccgccg gtggcagccg ttttgtcctt cccaaggccc
     3121 cacttccgca gcagccatgc catgatcatg atgcgcgctg ctccagccgg tcaccgctgt
     3181 tggcactctg cgtcagatcc ttgcgtttgg cctttccgct tttgacaggg cggacgttcg
     3241 ttggctattc gattgccttg tcggcgcggc catttgaaag gagtgtggca aagcgaacac
     3301 acctcaggtt tttcaaacga aggaaggcgc tttggccgct cgtcgcattg acgatcgaaa
     3361 ttaaaactac aaaggtagat tttgtcaccc cattgcgtgc gagaccatca tgatcg
//
LOCUS       DATLJT010000621         4380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2794827,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000621 DATLJT010000000
VERSION     DATLJT010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2794827"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VM910_14350"
     CDS             complement(<1..461)
                     /locus_tag="VM910_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI63747.1"
                     /translation="MASMNKVFADLPVTIFEAMSQAARDNDAINLGQGFPDDPGPEDI
                     RRAAADAVLNGYNQYPSMMGIPELRQAIAAHYGHWHGLKLDPMTEVMVTSGGTEALTS
                     AILAVVEPGDEVIVFQPVYDSYLPIIRQAGGIPRLLRLEPPHWRLSEEMLKG"
     gene            complement(471..800)
                     /locus_tag="VM910_14355"
     CDS             complement(471..800)
                     /locus_tag="VM910_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI63748.1"
                     /translation="MADHKVHDLAPEEVSKGMADGRYLLVDVREPNEVAAEAYPDGVV
                     VPLSTFDPQSIPDPKGKQVVFACRSGKRSVTASLAAQAAGLPYDKHLAGGILGWKAAG
                     LPTKTGG"
     gene            complement(925..2859)
                     /locus_tag="VM910_14360"
     CDS             complement(925..2859)
                     /locus_tag="VM910_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HVI63749.1"
                     /translation="MTTDVAAPAPDTTAASGQGDAHSTASFGALTLGSIGVVYGDIGT
                     SPLYALREAVVAASNGSEATVPAVLGVLSLILWSLIIVVTLKYVVFLMRADNHGEGGT
                     LALMALAQRGVTKAASAIVLLGIISAALFFGDAVITPALSVLSAIEGIKLLAPASEPY
                     VVPVTVVILLALFAVQSRGTARVAAFFGPIMLIWFLVIGFASFPAIVSQPEVLFSLNP
                     LYAVSFMLHHGIIGFVTLGAVFLAVTGAEALYADLGHFGKKPIQTAWLFIVLPALALN
                     YLGQGALVIADPKVVDNPAFLPFYLMFPDWARGPMVVLAAIATVIASQAVITGAYSLT
                     RQAIQHGLMPRFEIRHTSESQSGQIFIPRINRMLLIAVMLLVLMFKSSSALASAYGIS
                     VTGTMVVTATMAFVVVWKVWRWSPFAAAALIAPFLFLDLTFLAANLLKVLEGGWVPLA
                     LGTWVMLLMYTWRRGSRLLFEKSRKLEFPLADLVAMLEKRPPQRVSGTAVFLTSDPNS
                     APTALMHSLKHYKVLHEKNVILTIETASTPRVDPSERVKLEQMSPTFTKVALKFGFME
                     TPNVPKALAIARKLGWQFDIMSTSFFLSRRALKPAAHSGMPRWQDHLFIALSRTANDA
                     TDYFQIPSGRVVEVGTQITI"
     gene            complement(3081..3908)
                     /locus_tag="VM910_14365"
     CDS             complement(3081..3908)
                     /locus_tag="VM910_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HVI63750.1"
                     /translation="MAKKKRGEAHSGGHGWFVTFADLMGLMMSFFVMLVAFSTQDQQK
                     LKIVAGSMRDAFGVQTARYSGIIESDGIPTRPRLKNTDHIPPEEASNTPTPDQQERSR
                     INGAKLKIDREFALASASLRQALQDMPELTEISKHIMFEETKQGLNLEIVDQDGRSMF
                     ADGSKEPYERTRRLIEKLAIPLKQTPLRVSIIGHTAAGFVPTRSDYGPFDLSADRANA
                     VRQILEREGLPASHIFAVSGKADTQPLFPDDPSLSANRRVTITLMREDPPLPPDLKP"
     gene            complement(3940..>4380)
                     /locus_tag="VM910_14370"
     CDS             complement(3940..>4380)
                     /locus_tag="VM910_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVI63751.1"
                     /translation="FVADGYDLEFIRDNLERDRDNFLMHLDEGSKIYRAVGDCAPAFG
                     MIGTLIGMVQMFANMTDPSKLGPFMATALLATLYGALVANLLCLPIADKLHGKLLDEE
                     TNRTLIIDGILMIRDSKSPTLVREMLLAYLPEKHRHEEGEPVPA"
BASE COUNT          909 a         1321 c         1366 g          784 t
ORIGIN      
        1 cccttgagca tctcctccga gagccgccag tgcggcggct cgagccgcaa caggcgtgga
       61 atgccgccgg cctggcggat gatcggcagg taggaatcgt agaccggctg gaacacgatc
      121 acttcgtcgc caggctcgac cacggcaagg atcgccgacg tcaaagcctc tgtgccgccc
      181 gacgtcacca tcacctccgt catcggatcg agcttaagcc catgccagtg accgtaatgg
      241 gcggcaatcg cctggcgtag ctcaggtatc cccatcattg aaggatattg attatagccg
      301 ttcagcaccg cgtcggcggc ggcgcggcgg atatcttccg gtcccggatc gtcgggaaag
      361 ccttgtccca gattgatggc gtcgttgtca cgcgccgctt gcgacatcgc ctcgaaaatc
      421 gtgacaggaa gatccgcaaa gaccttgttc atggaggcca tgagaaagct tcagccgccg
      481 gtcttggtcg gaagccctgc ggccttccag ccgaggatgc caccggcaag atgcttgtcg
      541 taaggaagcc ccgcggcctg cgccgccagc gaagccgtca ccgaacgctt cccggagcgg
      601 caagcaaaca caacctgttt tcccttggga tcgggaatcg attgtggatc gaaagtggac
      661 agcggcacca cgactccatc cggataggct tcggctgcga cttcgttggg ctcgcgaaca
      721 tcgaccagca gatagcggcc gtcggccatc cccttcgaca cctcttctgg cgccaaatca
      781 tgcaccttgt gatccgccac ttggacttcc tccaaccact taccgatatg ctaaattttc
      841 aggctgccgc ctcaaactga cccgaacctc gcccttcgcg cggcaaaaat caagcaccgc
      901 aacagtttag gcgagcgagc gaacctaaat ggtgatctgt gtcccgacct cgaccacgcg
      961 tccgcttgga atctggaagt aatcggtcgc gtcgttggcc gtgcggctga gcgcgatgaa
     1021 gagatgatcc tgccagcgcg gcataccgga atgtgcggcc ggcttcagcg cgcgccgcga
     1081 caggaagaac gaggtcgaca tgatgtcgaa ctgccagccc agcttgcgcg caatcgccag
     1141 cgccttgggt acgttgggcg tttccatgaa gccgaatttc agcgccacct tggtgaaggt
     1201 cgggctcatc tgctccaact tgacccgctc cgacggatcg acgcgcggcg ttgacgcggt
     1261 ttcgatggtg agaatgacgt tcttctcgtg cagcaccttg tagtgcttga ggctgtgcat
     1321 caacgcagtc ggcgcgctgt tgggatcgga ggtgaggaac accgccgtgc cggatacccg
     1381 ctgcggcggc cgtttctcca gcatcgccac gagatcagcg agcgggaatt ccagcttgcg
     1441 cgacttctcg aacagaagcc tgctgccgcg acgccacgtg tacatcagga gcatcaccca
     1501 agtgccgagc gcgagcggca cccagccgcc ctcgaggacc ttcaacaggt tcgccgccag
     1561 gaacgtgaga tcgagaaaca ggaatggtgc gataagcgca gcggccgcaa acggcgacca
     1621 tctccagact ttccagacca cgacaaaggc catcgtcgct gtgacgacca tggtcccggt
     1681 gacggaaatg ccataagcgg aggccagcgc gctcgacgac ttgaacatca ggaccagcag
     1741 catcaccgcg atcaggagca tccggttgat gcgcggaatg aaaatctggc cggattgcga
     1801 ttccgaagta tggcggatct cgaagcgggg catcagtccg tgttggattg cctggcgcgt
     1861 cagcgaatag gcgccggtga tcacggcctg gctcgcgatc acggtcgcga tcgccgcaag
     1921 aaccaccatt ggcccccgcg cccaatccgg aaacatcagg tagaacggaa ggaaggccgg
     1981 gttgtcgacg accttggggt ccgcgatgac cagcgcaccc tggcccaaat agttcagtgc
     2041 caacgccggc aacacaatga acagccaggc cgtctggatc ggcttcttgc cgaaatggcc
     2101 gaggtcggca tagagcgctt cggcgccggt cacggcgaga aaaacggcgc ccaatgtaac
     2161 gaaaccgata atgccgtgat ggagcatgaa cgagaccgca tagagcgggt tgagcgagaa
     2221 aagcacctca ggttggctca caatcgcggg aaatgatgca aacccgatca cgaggaacca
     2281 gatcagcatg atcggaccga agaacgcggc gacgcgggcc gtgccgcgcg actgcacggc
     2341 aaacagcgca agcaggatca cgaccgtgac cggcacgaca tagggctcgg aggctggcgc
     2401 gaggagcttg ataccttcga tcgccgacag cacggacaac gcaggcgtga tcaccgcgtc
     2461 gccgaagaac agagcggcgc tgatgatccc gagcagaacg atggcgctag ctgctttggt
     2521 gaccccgcgc tgcgccagcg ccatcaacgc cagcgtgccg ccctccccgt ggttatccgc
     2581 acgcatgagg aacacgacgt atttcagcgt cacaacgata atcagcgacc acaggatcag
     2641 cgacagcacg ccgagcacgg ccgggacggt ggcttccgag ccgttgctgg cggccaccac
     2701 cgcctcgcgc aacgcataaa gagggctggt tccgatgtcg ccgtagacga ccccgatgct
     2761 gccgagtgtc agtgcgccga agctcgctgt ggaatgggca tcaccctgcc cgctggccgc
     2821 cgtcgtatcc ggagcgggcg ctgcaacgtc ggtggtcatg ggaactgtac ttcgctacgt
     2881 ccttcgcaag agaggcggca tatactcttg cgcttgcaat ctagcctagc ccgaatttgg
     2941 tgggtcggca tgcgaaaagg cataaatctt gggattgcga tggtgtgccg cgggcaccgc
     3001 cggcaagatg aaacaaccag cccggccccg acgaccaatt ggaagaaaag aacctgcgaa
     3061 ggtaatttag tagtgattgt ttacggcttc aagtcgggcg gaagtggggg atcctcacgc
     3121 atcagggtaa tggtgacacg ccggttggca gataacgagg gatcgtccgg aaagaggggt
     3181 tgggtatccg ctttgccgga gacagcgaag atgtgggagg ccggcaagcc ctcgcgctcg
     3241 aggatctgcc ggacggcgtt ggcgcgatcg gcggaaagat cgaacggccc gtagtcgctg
     3301 cgcgtcggca cgaaccccgc cgcggtatgg ccgatgatgg acactctgag cggcgtctgc
     3361 ttgagcggga tcgcaagctt ctcgatcaat cgccgggtgc gctcataagg ctccttcgag
     3421 ccgtcggcga acatcgaacg accatcctgg tcgacgattt cgagatttag cccctgcttg
     3481 gtctcctcga acatgatgtg tttcgagatt tccgtcagct ccggcatatc ctgcaaggcc
     3541 tgacgaagcg aagcggacgc cagcgcgaat tcgcgatcga tcttgagttt ggcgccgttg
     3601 atccggctgc gctcctgctg atccggggtc ggcgtgttgg aggcctcttc cggggggatg
     3661 tggtcggtgt tcttcagccg cggtcgcgtc ggaatgccgt cggattcgat gatgccggaa
     3721 tagcgcgcgg tctgcacgcc gaaggcgtcg cgcatcgaac cggcgacaat tttgagcttt
     3781 tgctgatcct gggtcgagaa ggcgacgagc atcacgaaga agctcatcat tagccccatc
     3841 agatcggcaa aggtcacgaa ccagccgtgg ccgcctgaat gagcctcgcc gcgttttttc
     3901 ttagccatcg cgtgcttccg ggatcctgac cagcgccgct caggccggta ccggctcgcc
     3961 ttcctcgtgt cggtgtttct ccggcagata ggccagcaac atctcgcgga ccagcgtcgg
     4021 gctcttggaa tcgcggatca tcaaaatgcc gtcgatgatc agcgtgcgat tggtctcctc
     4081 gtcgagcagc ttgccatgca gcttgtcggc gatcggcaga caaagcaggt tcgcgacaag
     4141 cgcgccgtaa agcgttgcga gcagcgcggt cgccatgaac ggtccgagtt tcgaggggtc
     4201 ggtcatgttg gcaaacatct gcaccatgcc gatcagcgtg ccgatcatgc cgaaggccgg
     4261 cgcgcagtcg ccgacagcgc gatagatctt gctgccctcg tcgagatgca tcaggaaatt
     4321 gtcgcgatcg cgctccaggt tgtcgcggat gaattcgagg tcgtagccgt cggcgacgaa
//
LOCUS       DATLJT010000622         5927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3024196,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000622 DATLJT010000000
VERSION     DATLJT010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024196"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VM910_14375"
     CDS             <1..275
                     /locus_tag="VM910_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI63752.1"
                     /translation="GNNRAMIMHNHGVLTVGKTAREAFILMKHLLEAAEIQLAMEATG
                     GELIEIPPEICKKTVQQYEKHDSGRGSADWPAYLRMLDRIDPSYRT"
     gene            278..1285
                     /locus_tag="VM910_14380"
     CDS             278..1285
                     /locus_tag="VM910_14380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63753.1"
                     /translation="MAKPFCRMVRGRSALLAALAVLAIVGLSQSARSQVWPQKPVKIL
                     VPFAPGGNSDGIARLIAQPLGDAFGQQFVVENRPAAGGAIAAEAVARSSADGHMLLMG
                     SPSQIVIIPLTTKTAYDPVKDFAPISVVGTNPYVLVVHAGIPATTLAEFVDYARGRPG
                     KLTYVAPIFGGLSHLSMVLFLKRAGLEMTPVSYKGGAAPLTDVIAGHVPIYFATLSEA
                     MSQANSGAIRLLAVSSAKRVPQIPDVPTFAESGFPGFKALTWNGLMAPSAMPTDTVER
                     IAKEVSHAAKDATFAERLAAFGVDPLGNTPAAFAAMIAADIALWGEAVKIAGIQTHRA
                     N"
     gene            complement(1436..2233)
                     /locus_tag="VM910_14385"
     CDS             complement(1436..2233)
                     /locus_tag="VM910_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI63754.1"
                     /translation="MAWQTIEAPPGVSPPHPITVRAQSIPPRHHFPEHSHRWNQLAYA
                     ISGVLTVTVEGHSFVISSEQAVWLPTDLRHRVGSLLGAEFRSLWIAEEAGRDLPASPT
                     VFAVTGLLKALIIEATAIEGKKDSDGYASRVTDLILDQLRRARPLPGALPWPRGGSSL
                     TDLCEALYADPANDRGPEAWGRELGMSERTLARRFEAEVGMSFRDWRRRLRLFKAIEL
                     LGGGLDVTRTAMELGYGSTSAFVYAFRTQMGRSPQAYMRGRAPLKRS"
     gene            2361..2552
                     /locus_tag="VM910_14390"
                     /pseudo
     CDS             2361..2552
                     /locus_tag="VM910_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2681..3874
                     /gene="soxC"
                     /locus_tag="VM910_14395"
     CDS             2681..3874
                     /gene="soxC"
                     /locus_tag="VM910_14395"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HVI63755.1"
                     /translation="MAGGAIAVGLRKAADAAPPDVPDSMRSLGQSVLSPPYGVPSKFE
                     KDVVRRLREPRATDTESFSVTPLQNLHGIITPNGLVYERHHAGIPEIAPSEHKLLVHG
                     LVDRPLIFSIDDLLRFRSVSRIHFLECSGNSSTEWGKSGYHSVQRTHGLISCCEWTGV
                     PLSTILDEVGLKPEAKWVLAEGADAAAMTRSVPLEKALDDALVVYAQNGEMLRPEQGY
                     PVRLFLPGFEGNMSVKWLRRLKVGDQPWHTREETSRYTNLLPNGKAYQFAFGMDVKSV
                     VTFPNSERTFTDKGFYEISGLAWSGRGHVTRVDVSVDGGASWQRCMLQEPILEKCFTR
                     FRLPFVWDRQPREIQSRAFDDKGWVQPTRASLIAARGTNARYHFNAIQGWKVAADGGV
                     TNAST"
     gene            3861..4427
                     /locus_tag="VM910_14400"
     CDS             3861..4427
                     /locus_tag="VM910_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI63756.1"
                     /translation="MRALNITVALAGLLWVDVASAQHARVGFGTPATPDQIGGWDIDV
                     RPDGWGLPQGAGTVERGEKIYAEQCAACHGEKGQKPAKGFDQLAGGRGTLASPKPVRT
                     IGSNWPYATTIFDYVRRAMPFPAPQSLTTDEVYSVVAYLLYINDILPKEAVLNAQTLP
                     KVRMPNADGFIPDARPDVDDMKCKRDCQ"
     gene            4726..5919
                     /locus_tag="VM910_14405"
     CDS             4726..5919
                     /locus_tag="VM910_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVI63757.1"
                     /translation="MLDVVKKVAPSIPFDADRLDRLMDEAGIDVLLATSKHNVQYLLG
                     GHRAFFFESMDAMGLSRYLPVLVYPKGARQKAGYFGHRMEGFQKENDPFWVSESQTNS
                     SGTVDTMQKAIDYVRKLGAKPRRIGVELAFLPVDAGTALRNAFPDSEIVDALFVLERL
                     RAVKSPEELRKLRIASERVIESMLAVIANHGPGATKRELTEALRREETSRGLTFDYCL
                     ITAGASLNRAPSEQKWGKGDVLSLDSGGNYHGYIGDLCRMAIHGEPDAELEDLLAEIE
                     RIQRAAMKPIKAGAMGSVIYAAAEPLVHKSKHHNHMHFLAHGMGLVSHEAPRLTATGP
                     VPYDAYDADRPLESAMVVSVETTLQHPQRGFIKLEDTVVVTDTGFEIYGEGGRGWNRG
                     GTALR"
BASE COUNT         1146 a         1822 c         1858 g         1101 t
ORIGIN      
        1 tcggcaacaa tcgcgccatg atcatgcaca atcacggcgt gctcaccgtg ggcaagaccg
       61 cgcgcgaggc tttcatcctg atgaagcatc tgttggaggc cgctgaaatc cagttggcca
      121 tggaagcaac cggcggcgag ctcatcgaga tcccgcccga aatctgcaag aagacggtgc
      181 agcaatacga aaaacacgac agcggccgcg gctccgccga ctggccggct tatctgcgca
      241 tgctcgaccg catcgatccg agctaccgga cctgactttg gcaaaaccgt tctgcaggat
      301 ggtgcgtggg cgaagcgcgc ttctggcagc gctggccgtg ctcgccattg tgggtttgag
      361 tcagtccgca cgctcgcaag tctggccgca gaagccggtc aagatcctcg ttccctttgc
      421 gcctggcggc aatagcgacg gcattgcccg tctgatcgcg cagcccctcg gcgacgcgtt
      481 tgggcaacaa ttcgtggtcg agaaccgccc agcggcgggc ggcgcgatcg cggccgaagc
      541 ggtcgcccgt tcgtctgccg acggacacat gctgctgatg ggcagcccgt cgcaaatcgt
      601 aatcattccg ctgacgacga agacggccta cgatccggtc aaagattttg ctcccatcag
      661 cgtcgttggg accaacccgt atgttctggt cgtccacgct ggcataccgg cgactacgct
      721 tgccgaattc gtcgactatg cccgcggccg gcctggcaaa ttgacctatg tggcccccat
      781 attcggcggc ttgagccatc tctcgatggt gctgtttctc aaacgcgccg ggctcgagat
      841 gaccccggtg agctacaagg gcggtgcggc gccattgacc gacgtcatcg ccggtcacgt
      901 ccccatttat ttcgcgactt tgtcggaggc gatgtcgcag gcaaacagcg gcgccattcg
      961 cctgcttgcg gtgtcgagcg cgaagcgcgt cccgcagatc ccggacgttc ccaccttcgc
     1021 cgagtcggga tttccgggat tcaaggcgct cacctggaac gggctgatgg cgccatcggc
     1081 gatgcccacg gacaccgtcg agcggatcgc gaaagaggtg tcccacgccg ccaaggacgc
     1141 gacattcgcc gagcggcttg cggcgttcgg ggttgacccg ctcggcaaca cccccgcggc
     1201 gttcgcggcg atgatcgccg ccgacatcgc tttgtggggg gaagcggtca agatcgccgg
     1261 catccaaacg catagagcga actaggccgt gtactcataa acgcgagcac tggccgaatg
     1321 tctgcttttg ggggcgaacc ggacacgccg cgatggcggg aatgacgtga atgacccatt
     1381 ccagacgtca gtccgcctgc gcagcagtgc cgggaagttg gccaatggtt gccggtcagc
     1441 tgcgcttgag cggcgcacgt ccgcgcatgt aggcttgggg actgcgaccc atctgggtgc
     1501 ggaaggcgta cacgaaagcc gacgtggagc cgtagccgag ctccatcgcg gtgcgggtta
     1561 cgtccaagcc gccgcccagc agttcaatgg ccttgaacaa ccgtaagcgt cgccgccagt
     1621 cccggaagct catgcccacc tcagcctcga agcgccgcgc cagggtccgc tccgacattc
     1681 cgagctcgcg accccaagct tccggaccgc gatcgtttgc tgggtccgcg tagagcgcct
     1741 cgcacaagtc ggtcagagac gatcccccgc gcggccaagg cagcgcgcct ggaagaggac
     1801 gtgcgcgtcg tagctggtca aggatgaggt cagtcactcg gctcgcatag ccgtcggaat
     1861 ccttctttcc ctcgatcgcg gtggcttcga tgatgagagc cttcagcaat cccgtgacgg
     1921 cgaagacggt cggactcgcg gggagatcac gaccggcctc ctcggcgatc cagagactgc
     1981 ggaattcggc tccgagcagt gatccgactc ggtgccgaag gtctgtcggc aaccagacgg
     2041 cctgctccga cgagatcaca aatgagtgcc cttccaccgt caccgtcagc accccggaaa
     2101 tggcgtaagc caactggttc cagcggtgcg aatgctccgg aaaatggtgg cgcggcggta
     2161 tcgactgcgc gcgcacggtg atcgggtgag gcggcgagac ccccggtggg gcttcgattg
     2221 tttgccatgc catcggcggc gtccactttg gcggaaactc tacatcattt ggcagactat
     2281 caaaatacgg acagtaggta ttctcatata agtcaggccc acgagggcag ggtccccctt
     2341 tcacaaaatg actgatctcg atgtcacacg ctgaagccac ggctccggtc ggtctcgtac
     2401 ccgcgctccg ttcgggcggt gccgatgtgc tgaggcttgc ggttgcgcag gcgcttgccg
     2461 gggcgaattc cgccatcgtg tacgcaaccg gtgccgtcgt cggcaacacg cttgccccga
     2521 gcaaggcgct cgcgcttgtg tggcctgtcg caggactcat ccaacttagg cctgcaacgg
     2581 cacatcatcg cgatgtatgc gcctagcttt ttcacctgac ctcaggagag cgcccgtgac
     2641 aatcagtaga cgcaacgtgc tgaggtcgtc cgcgacaata gtggctggcg gcgctatcgc
     2701 agtagggctc cgcaaagccg ccgacgcagc gccgccggat gtgccggatt cgatgcgtag
     2761 cctgggccaa tcggtcctct ctccccctta cggcgtgcca tcgaaattcg agaaggacgt
     2821 cgttcgccgg cttcgagagc cacgcgcgac tgacactgaa tcgttcagcg tgacgcctct
     2881 gcaaaacctg cacggcatca tcacaccgaa tggtctggtg tacgagcgcc atcacgccgg
     2941 catccctgaa atcgcgccct ccgagcataa gctccttgtt catgggcttg ttgaccgacc
     3001 gcttatcttt tcgattgacg atctcctacg gttccggtcg gtctcgagaa tccacttcct
     3061 ggaatgctcc ggtaacagct caaccgaatg ggggaagtcg gggtatcact cagtccagcg
     3121 cacacacggc ctgatctcct gttgtgaatg gacaggtgtg ccgctctcca cgatcttgga
     3181 tgaagtcggg ctaaagcccg aagcaaaatg ggttcttgcg gaaggggctg acgcggctgc
     3241 aatgacgcgc agcgtgccgc ttgaaaaagc gctcgacgat gccctcgttg tttacgctca
     3301 gaacggcgag atgcttcggc cagaacaggg ctatccggtg cgactgttcc tgcccggttt
     3361 cgaaggcaac atgtccgtta aatggctgcg tcgattgaag gttggcgatc aaccttggca
     3421 cacgagagag gaaacttctc gctacactaa ccttcttccg aacggaaaag cgtatcagtt
     3481 cgcgttcggg atggacgtga aatcggtcgt gacatttccg aactcggaac gcaccttcac
     3541 ggacaaaggc ttctacgaaa tatccggact agcctggtcc ggtcgcggcc acgtcactcg
     3601 ggtcgatgtc tcggtcgatg gcggggcgag ttggcagcgc tgcatgcttc aggagcccat
     3661 cctcgaaaaa tgcttcacgc gctttcgact gccattcgtc tgggatcgac agccacggga
     3721 gatccaaagc agggcgttcg atgacaaagg ctgggtgcag ccaacgcgcg ccagcctgat
     3781 cgcagcgcgc gggactaacg cccgttatca cttcaatgca attcagggct ggaaggtcgc
     3841 ggctgacggt ggggtgacca atgcgagcac ttaacatcac ggttgccctc gcagggctct
     3901 tgtgggtgga cgtagcttct gcgcagcacg ctcgcgtggg ctttggcacc cccgccacgc
     3961 cagatcaaat tgggggttgg gatattgacg tgcggcccga tggctgggga ctgccacagg
     4021 gtgctggcac ggttgagagg ggtgagaaaa tttacgcgga gcaatgcgcg gcgtgccacg
     4081 gcgagaaggg gcaaaagcca gcgaaaggct tcgaccagct cgctggcggc aggggtacgc
     4141 ttgcttcgcc gaaaccagta cggaccatcg gcagcaattg gccgtacgcc acaaccattt
     4201 tcgattacgt ccgtcgcgct atgcctttcc ctgcaccgca aagcctcacc accgatgaag
     4261 tgtactcagt cgtggcgtat cttctctata taaacgacat cctacctaag gaggctgtcc
     4321 tcaacgcaca gacactcccg aaggtgagga tgccgaacgc agatggcttc atcccagatg
     4381 cgcggcctga cgtggacgat atgaagtgca agcgtgactg ccaatgatgc cgtgacttca
     4441 ggggtccttt gtgggctcat ggcacaaagc ggacataccg attttgtccg ctttcggagg
     4501 caaggcggac atcggccagc gattgcttac caatcacgga tttatgagta cacgccctag
     4561 cgggacgtgg tgccgcggcg acacgcggcg gcgttgcagg aatgccgcgg gcgccggctg
     4621 ggcatggctg gatcgcgccg ccttggtgta tgatcgcagg gttccgctca cccatgcgcg
     4681 cgcagcgcac ggaacgttgc gatccgatta caaggagctt cggtcatgct cgacgtcgtc
     4741 aagaaggttg caccctcgat tccattcgat gcggatcgct tggatcggct gatggacgaa
     4801 gccgggatcg acgtcctgct cgcgacctcg aagcacaacg tgcagtacct gctcggcggc
     4861 catcgcgcct ttttcttcga gtccatggat gcgatgggat tgagccgcta tctgccggtc
     4921 ctcgtctatc ccaaaggcgc gcggcagaag gcgggttatt tcggccatcg catggaaggc
     4981 tttcagaagg aaaacgatcc gttttgggtg tcggagtcgc agaccaattc gtccggcacc
     5041 gtcgatacca tgcagaaggc gatcgattac gtgcgcaagt tgggagcaaa gccgcgccgg
     5101 atcggcgtcg agctcgcctt cctcccggtc gatgccggca ccgcgctacg caacgccttc
     5161 cccgacagcg aaatcgtgga cgcgctgttc gtgctcgagc ggctgcgcgc ggtgaagagc
     5221 cccgaggaat tgaggaagct gcgcattgct tccgagcgcg tgatcgaatc gatgctggcg
     5281 gtgattgcca atcacggccc gggcgcgacc aagcgcgagc tgaccgaagc gctgcggcgg
     5341 gaggagacca gccgcggtct cacattcgac tattgcctga tcacggccgg cgcgagcctc
     5401 aaccgcgcgc cgtcggagca gaagtggggc aagggcgatg tgctctcgct cgattccggc
     5461 ggcaactatc acggctatat cggcgacctc tgccggatgg cgattcacgg cgagcccgat
     5521 gccgagctcg aagatctgct ggcggagatc gagcgcattc agcgcgcggc gatgaagccc
     5581 atcaaggcgg gagcgatggg gagcgtgatc tacgccgcgg ccgaaccgct cgtgcacaaa
     5641 tccaagcacc acaaccacat gcacttcctc gcccacggca tgggattggt cagtcacgag
     5701 gcgccgcggc tgacggcgac cggcccggtg ccatacgatg cctacgacgc cgatcgcccg
     5761 ctcgagagcg caatggtggt ctcggtcgag accacgctgc agcatcccca gcgcggattc
     5821 atcaagctcg aagacaccgt cgtggtcacc gacaccggat tcgagattta cggcgagggc
     5881 ggtcgcggct ggaaccgcgg cggcaccgcg ctgcggtgag ttggcca
//
LOCUS       DATLJT010000623         6607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2565355,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000623 DATLJT010000000
VERSION     DATLJT010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565355"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..590
                     /locus_tag="VM910_14410"
     CDS             264..590
                     /locus_tag="VM910_14410"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HVI63758.1"
                     /translation="MSGLGLECEAQVSDMPENRRETEKRRKVKFRKGQSGNPGGRPKV
                     LGEVQELARQYAPKAIAELARLARRQRVGHNAVPSVLAPRLLLLCDPVTWISGGKRSG
                     LPCLGE"
     gene            617..1405
                     /locus_tag="VM910_14415"
     CDS             617..1405
                     /locus_tag="VM910_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI63759.1"
                     /translation="MRIPKSFSRAEEQALKPKDLFKECDNCPEMIVVPAGEFMMGSPE
                     DEEGHTEDESPQHKVQLLTPFAVGRFAVTFEEWDACVADHGCRKYMPPDRGWGRGRQP
                     VIAIWWDDAIAYVRWVSQKTGRNYRLLSEAEREYVARAGTTTPFWWGNSIFSNRANYA
                     SSRGGEVQRKTLPVDSFEPNPWGLYQVHGNVSEWIGDCWHYDYEGAPLDGTAWTSIDC
                     ERPMLRGGNWNSTPWELRSASRGRWASAALFLPAIGMRVARPLR"
     gene            1736..4930
                     /locus_tag="VM910_14420"
     CDS             1736..4930
                     /locus_tag="VM910_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI63760.1"
                     /translation="MLKRATTFGLRQRLLLSFIAISGFAVVAAVVGNYAFYAIGEALR
                     QVTAITVPPAIASLEFAQRIERIVAAGPALLAATNNAELAGASRAVEQQVKDAAQLLN
                     DLPDQAVTVDKLIEIRTVFNQVAVNLSALKSAAQRRIDATDRKAMLVRDTSDAYNQFR
                     TFLTPKFEELKGQIAALQNSIRFLARGSTEERRATDRLNAATSNLAPLEQIQQEAAIA
                     FESLVHAANASTGLNTIQTQVERSVRRIDNLVSGLDPDISHDLIVPLSRLRAGAIGSS
                     SIMAARQVELETAAEGNRLTAENSVLRVQLGDAVAALVAGSKRGIATATEQVESVQKL
                     GRIGLLAVVALSLISSALIVWLYVGRNIVARLTALSDRMLTLAQGDLKSSLPQGGTDE
                     IGRMAEALSVFRATAIEMEEANLKEIREARARLTEAIETISEGFSLYDAEDKLIVCNS
                     HYRELFASHAEVMVPGTSFETILRTATGRGLIKDAEGREEAWIAERLERHRASSETHM
                     QRRSDGRWIQVSERKTANGGVVAIYADITQLKQHEAELAAARDAADEANRSKSSFLAT
                     MSHEIRTPMNAVIGMSGLLLDTTLNDEQRDYASTIRDSSEALLTIINDILDFSKIEAG
                     RMDIEAQPFDLRDCVESALDLISSRAAEKGLDIAYLFEGDMPAAIQGDLTRLRQILLN
                     LLSNAVKFTDKGEVVLTVTSEGDQLKFAVRDTGIGLDENGKSRLFQSFSQADSSTTRK
                     FGGTGLGLAISKRLAELMGGTMWVESAGPGHGSTFRFTIRGQRADLPQGRRRDFIGAQ
                     PALQGKRILVVDDNATNRKILALQAVKWGMGVEDTEAPEQALQILKRGSFDLAILDMH
                     MPGMDGATLAVRIREAGYTLPLVLFSSVGHKEVSDSLFAARLAKPLRQSQLFDTLVTL
                     LVHDAAPRQVAARAKSRIDAEMASRHALRILLVEDNAVNQKLALRLLQQMGYRADLAS
                     NGIEAIECVARQTYDVVLMDVQMPEMDGIEASRRITARWPTGQRPRIVAMTANAMQGD
                     REMCLAAGMDDYLSKPIRLDELVEALMCVPTRQE"
     gene            4937..5284
                     /locus_tag="VM910_14425"
     CDS             4937..5284
                     /locus_tag="VM910_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVI63761.1"
                     /translation="MDEPVIDRAVYAELRDTTGAEFLGELVDTFIEEGPGMLAELRGA
                     CADGNAERFRRAAHSLKTNGNTFGALKLAARARELELKGPDAESARDAAALATLEAEY
                     ARAVAELKALRNG"
     gene            5277..6320
                     /locus_tag="VM910_14430"
     CDS             5277..6320
                     /locus_tag="VM910_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI63762.1"
                     /translation="MAEDSSRLLIADDNKVNRLLLSRGVELLGHRFALAENGRIALEK
                     LRSEPFDLLLLDIEMPELDGFAVLEQLKADLHLRDVPVIVTSSVEGLDNIVRCINLGA
                     EDYLPKPVNAVLLKARIDASLEKKRMRDRQKAMVRRFATPEVADDLLQSDFALGGKRI
                     HASVLFSDIRGFTALLESQGPEETIDLLNTYYMLMFDAIGSHGGVVSQIMGDGLMALF
                     GAPLPLDDCAGSAVAAGLEMVELIEQFNLERSAVKKAAIRIGIGVATGDVVAGYTGTQ
                     QRATYTCIGDTVNLASRLEAHTKVAGHGILIDDATRRALGSSVPMQALGEVLFKGKAA
                     AVEIFAVAAAQPD"
BASE COUNT         1363 a         1941 c         2082 g         1221 t
ORIGIN      
        1 cgagagtctc gcggatcgcg gcttcgcaaa ggctcgcaac agcaccggtc accgcgggtt
       61 ccgcaacctt gccgtaaaaa cgcaatgaca gccggtctga caggttctga caccctttcc
      121 ggtttacacc gttacgcgtg cgcgcgcgta cggtggatag tgcaatcacc cgtcagaggc
      181 tgtcagagag tgccttggcg gcggccggtt cgcgcgaata ccaaacctgc cattagtctt
      241 gctgcgctcc tggaagctgt gcgatgtccg gtcttggcct tgaatgcgaa gcccaggtct
      301 ctgatatgcc tgagaacagg cgggaaacag aaaagagaag aaaagtgaaa tttcgcaagg
      361 gccagagcgg caatccgggt gggcgcccca aagttctcgg cgaggtgcaa gaactggcgc
      421 gccaatatgc tccgaaggct atcgccgagc ttgctcgcct ggcgcgccgc caacgcgtcg
      481 gccacaatgc tgtgcccagc gttctggctc cgcgcttgtt attgctttgt gatcctgtta
      541 cttggattag tggcggaaag cgctcaggtc ttccttgcct cggcgaatga aataactgag
      601 caattgacct tgggaaatgc gtattcccaa gtcgttctcg cgggcagaag agcaggcatt
      661 gaagcccaaa gatctattca aagaatgtga caactgccct gagatgatcg tcgtgccagc
      721 aggcgagttc atgatgggct ctccggaaga tgaggaggga cacaccgagg acgaatcacc
      781 ccagcacaag gtgcagctgc tcacaccgtt cgcggtcggt cggttcgcgg ttacgttcga
      841 agagtgggac gcatgcgtag ccgatcacgg ctgcaggaaa tatatgccgc cggacagagg
      901 ttggggccgc ggccgccaac ccgtcatcgc catttggtgg gacgacgcca tcgcatatgt
      961 gagatgggtc tcgcagaaga ctggtagaaa ctaccgactg ctcagtgaag cagagcggga
     1021 gtacgtcgca cgagcaggga caacgactcc attctggtgg gggaattcaa ttttcagcaa
     1081 tcgggccaat tacgcttcca gccgcggagg agaggttcaa cggaaaactt tgcccgttga
     1141 ttcttttgaa ccaaatccat ggggcctgta ccaggtgcat ggcaatgtct cggagtggat
     1201 tggtgactgc tggcattacg attatgaagg tgcgccgttg gatggtacgg cgtggaccag
     1261 tattgattgc gaacggccga tgctgcgcgg cggtaactgg aattccacac catgggagct
     1321 gcgctccgcg tcgcgtggga ggtgggcctc cgctgcactt tttttacccg ctatcggaat
     1381 gcgggtcgca cggccgcttc gatgagcgtg ctgcaatgcg cttgcagcga gagatcatgc
     1441 gccgagctaa cccatcgacc gtgctatcat gaaccgttag ggggaggaaa tctgatgccc
     1501 gtcggtcaaa ccaaccggcg cggtcgtccg cctagggagt gctaggtgag acggcgccgg
     1561 gtcacgagtc gcgaggcagc gaaggcacag ccgacgatca aggggaagcg cagcgacgcg
     1621 tcaaaggccc acgcactgag tacggcaggt cacaaaacca gactgccgcc gcggcagcat
     1681 gaggtccgat aatggcaagt ttcttatttg gcgaaagtga acgaggaggg cgaatttgct
     1741 gaaacgagcg acgacgttcg gcttgcgtca gcggctcctg ctgtcgttca ttgcaatcag
     1801 tggcttcgcg gtagtcgctg cggtggtcgg gaactacgcc ttctacgcca tcggcgaggc
     1861 tctccgccaa gtaacggcga taactgtccc tccggccatc gctagcttgg agttcgcgca
     1921 acgtatcgaa cgtatcgtcg ccgctggacc cgcactgctt gccgcaacca ataacgcgga
     1981 gcttgccggc gcttccaggg ccgtcgagca acaagtgaag gatgccgcac aactgctcaa
     2041 tgatctaccc gatcaggctg tcaccgtcga caagcttatc gagatccgca ccgtcttcaa
     2101 ccaggtagcc gtcaatcttt cagcattaaa atcggccgcg cagcgccgta tcgacgctac
     2161 cgaccgtaaa gccatgctgg tccgcgacac gtccgacgcc tacaatcaat tccgcacgtt
     2221 cttgactccg aaattcgagg aactgaaggg ccagatcgcc gcattgcaga actcaatccg
     2281 ttttttggca cggggcagca ccgaggagcg acgggctacc gaccggctca acgcggcgac
     2341 tagcaatctc gccccgctcg agcagattca acaggaggcc gcgatcgcgt ttgagtctct
     2401 tgtccatgcc gccaacgcca gcacaggtct caacaccatt cagactcaag tcgaacgctc
     2461 ggtcaggcgc attgacaatc tggtgtcggg tctcgatcct gacatctcgc atgacctgat
     2521 cgttccgctc agccggttgc gggccggtgc gatcggaagt tcgagcatca tggccgctcg
     2581 acaggtcgag ctcgaaaccg cggcggaggg caatcggctc acggccgaga actcagtatt
     2641 gcgggttcag ttgggcgatg cggttgccgc cctggtcgcc ggatcgaaga gaggaattgc
     2701 gacagccacc gagcaagtgg agtccgtcca aaaattggga cgcataggtt tgctcgccgt
     2761 tgtcgcgctc agcctgatca gctcggcgct gatcgtttgg ctttatgtcg gccgcaatat
     2821 tgtcgctcgc ctcacggcgc tcagcgatcg catgctcacc ttggcccagg gcgatctcaa
     2881 atcgtcgctt ccgcagggcg gcaccgatga gatcggccgc atggcagagg cgctgagcgt
     2941 attccgcgca acggccatcg agatggaaga ggcgaatcta aaggaaatcc gtgaagcacg
     3001 cgcccgcctc acagaggcga tcgagaccat atccgaaggg ttctcgcttt acgacgccga
     3061 agacaagctc atcgtgtgca atagccatta tcgagagctc ttcgcatctc atgccgaggt
     3121 gatggtgccc ggcacgagct ttgaaacaat tttgcgcact gccaccggac gcggcctgat
     3181 caaggatgcc gagggacgcg aggaggcctg gattgcagaa cgacttgagc gccaccgcgc
     3241 ctcgagcgaa acgcacatgc aacgccgcag cgacggccgc tggattcaag tcagcgaacg
     3301 aaaaacagcc aacggcggcg ttgtcgcgat ctatgccgac atcacccagc tgaagcaaca
     3361 cgaagccgag cttgccgccg cgcgcgatgc cgccgacgaa gccaaccgga gcaaatcgag
     3421 cttcctcgcg acgatgagcc acgagatccg cacgccgatg aatgcggtta tcggtatgag
     3481 cggccttttg ctcgacacta cgctcaacga cgagcagcgc gattatgcca gcaccatccg
     3541 cgattccagc gaagcgctgc tcactatcat caacgacatt ctcgacttct cgaagatcga
     3601 agccgggcgc atggacatcg aggcccagcc cttcgatctg cgcgactgtg tggagtcagc
     3661 gctggacctc atcagcagcc gcgctgccga gaagggcctg gacatcgcgt atttgttcga
     3721 aggtgacatg cccgcggcta tccagggtga cctcacccgc ctgcggcaga tcctcctgaa
     3781 tctgttaagc aacgcggtga agttcaccga caagggcgaa gtggtgctga ccgtcacatc
     3841 cgagggcgac cagttgaagt ttgccgtgcg cgacaccgga atcgggctgg atgaaaacgg
     3901 caagagccgt ctcttccagt cgttctcgca ggctgactcc agcactacgc gcaagttcgg
     3961 cggcaccggg ctgggcttgg cgatcagcaa gaggctggct gaactgatgg gaggcacgat
     4021 gtgggtggaa agcgccggcc cgggacacgg cagcactttc cgtttcacaa tccgtggcca
     4081 gcgtgccgat ctgccgcaag ggaggcgacg tgacttcatc ggcgcgcagc cggccctgca
     4141 aggcaagcgc atcctggtgg ttgatgacaa tgccaccaac cgtaagattc tggcattgca
     4201 ggcggtcaaa tggggcatgg gggttgagga caccgaagca cccgagcaag cgctgcagat
     4261 tctcaagcgc gggtccttcg atttggcaat cctcgatatg catatgcccg gtatggacgg
     4321 cgccacgctg gctgtacgca tccgcgaagc cggctacacg ctgccgctcg tgctgttcag
     4381 ctcagttggc cacaaggagg ttagcgacag tcttttcgcc gccaggctgg ctaaaccgct
     4441 gcggcagagc cagctctttg acacgttggt gacgctgctg gtgcacgacg ccgcgcccag
     4501 acaggttgcc gcgcgagcca agtcccgcat cgacgccgag atggcctcac gccacgcgct
     4561 tcgcatcctt ctggtcgagg acaacgcggt gaatcagaag ctcgcgcttc gcctgctgca
     4621 gcaaatgggc taccgcgccg acctggccag caatggcatc gaggccatcg agtgcgtcgc
     4681 tcgccagact tacgatgtgg tgctgatgga cgtgcagatg cctgagatgg acggcattga
     4741 ggcctcgcgc cgcatcacag cgaggtggcc caccggccag cgcccgcgca tcgtcgcaat
     4801 gaccgccaac gccatgcagg gcgatcgcga aatgtgtttg gcggcgggca tggacgacta
     4861 cctgagtaaa ccgatccggt tggatgagct ggtcgaggcc ttgatgtgcg taccgacacg
     4921 ccaggagtga ggaaacatgg atgaaccagt gatcgaccgg gctgtctatg ctgagctgcg
     4981 ggacacgacg ggtgccgagt tcttgggcga gctggtggac acgtttatcg aagaggggcc
     5041 cggcatgctt gccgagttgc gcggcgcctg tgccgacggc aatgctgaac gcttccggcg
     5101 cgcagcgcac tcgctcaaaa ccaacggaaa caccttcggc gcactgaaat tggcagcgcg
     5161 ggcgcgcgag cttgagctca aagggcctga tgccgaatca gcccgcgacg cggccgcctt
     5221 ggcgacgctg gaggcggaat acgcgcgtgc cgttgccgaa ttgaaggcgc tgcgtaatgg
     5281 ctgaagacag ttctcgtctg ctgatcgccg acgacaataa ggtcaatcgc ttgctgctgt
     5341 cgcgcggcgt cgagctgctg gggcatcgct ttgcgctggc tgagaacggc cgcatcgcgc
     5401 tggagaaatt gcgaagcgaa ccgtttgacc tcctgctgct cgacatcgaa atgcccgaac
     5461 tggatggttt cgcggtcctt gagcagctca aggccgacct gcatttgcgt gacgtgccgg
     5521 tcatcgtcac ctcgtcggtt gaggggctgg acaatattgt ccgctgcatc aacctggggg
     5581 ccgaggacta tctgcccaag ccggtgaacg cggtgctgct gaaagcccgc atcgacgcca
     5641 gcctcgagaa aaagcgcatg cgcgaccggc agaaggcaat ggtgcggcgc ttcgccaccc
     5701 ccgaggtggc ggatgacctg ctgcagtcgg acttcgcgct cggcgggaaa cgcattcacg
     5761 cgtcggtgtt gttctctgac atccgcggct tcacggcgct gctcgagagc caggggccgg
     5821 aagagacaat cgatctgctc aacacgtatt acatgctgat gttcgacgcc atcggcagcc
     5881 atggcggcgt ggtcagccag ataatgggcg acggcctgat ggcgctgttc ggcgcgccac
     5941 tcccactcga cgattgtgcc ggcagtgctg tggcggcggg gctcgaaatg gtcgagctga
     6001 ttgagcaatt caaccttgag cgtagcgcgg tgaagaaggc agcaatccgt attggcatcg
     6061 gcgtcgcgac tggtgacgtc gtggccggct acaccggaac acagcagcgt gcgacctaca
     6121 cctgcatcgg cgatacggtg aacttggcgt cacggctgga agcgcacacc aaggtggctg
     6181 ggcacggcat cctgatcgat gacgctacac gacgtgcgct gggcagcagt gtgccgatgc
     6241 aggctctggg cgaggtgctg ttcaagggca aggctgctgc tgtcgagatt ttcgcggtgg
     6301 cggccgcgca gccagactaa tgcatcccca gacgaccgag cgcctttgca aacgcgacgc
     6361 agtcgtccag ggcagtctta ctaacccgct ttcgccctgg tcgcggtgcc ggctacgtcg
     6421 tattgcttaa ggaaggcgag catccgcgcc caggcgtcct tggctgcgtc ggcccggaat
     6481 cctagtggca ttgcgcggcg tcgcaatggc ggctgtaatc gccagccatg agcacgaaga
     6541 agcgcgaggt catcttcggc ggcaagatca tgggcgcgaa aatccgcgcc gagcacgcgc
     6601 gtgagtt
//
LOCUS       DATLJT010000624         9610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_955861,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000624 DATLJT010000000
VERSION     DATLJT010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955861"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VM910_14435"
     CDS             complement(<1..314)
                     /locus_tag="VM910_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HVI63763.1"
                     /translation="MKKLLRNTLRSKWFQGAVGFLAAEYLRLVWRTNRITMDPPNIQA
                     RVEAQMPAIFAFWHGQHFLTPFIRLKRYRAKVLISLHRDGEYNAIAAERLGVGTIRGS
                     GD"
     gene            complement(311..574)
                     /locus_tag="VM910_14440"
     CDS             complement(311..574)
                     /locus_tag="VM910_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HVI63764.1"
                     /translation="MPDNGPQQQLLHLVIGGELTDLENNIFKDLDRVDIVGVFPNYAT
                     AYAAWKARAQATVDNAHMRYFVVHLHRLLDPGNDPKQATKPTR"
     gene            complement(761..1576)
                     /locus_tag="VM910_14445"
     CDS             complement(761..1576)
                     /locus_tag="VM910_14445"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HVI63765.1"
                     /translation="MVDASGPFRTFALTRDADLLKDTVREAGELALSLFRTELRNWTK
                     GASSPVSEADIAVNDLLEQRLRAATPDYGWLSEESADDEHRLSKRLVWIVDPIDGTRG
                     YLAGREDWCVSAALVEGAAPVLAAVFAPASDEFFFAARGQGATRNDIALQATSGTELD
                     FSRVAGPKPLVERLNPSRGEIVLHPRIASLALRLCRVAQGSLDAAFAGGQSRDWDLAA
                     ANLIVQEANGNMTALSGDAILYNRREVTHGVLVAAGRDRHADIVSHFSKRPLP"
     gene            complement(1563..2963)
                     /locus_tag="VM910_14450"
     CDS             complement(1563..2963)
                     /locus_tag="VM910_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVI63766.1"
                     /translation="MISSPSTVSPLPAKSAGRDLFDQSALSALAERLVEAARRAGADA
                     ADAVAVRGVSQAVELRDGRVEESERSEGDDVGLRVLVGQRQAVVSTNDVSGDGIARLA
                     ERAVAMARVAPDDKYVGLADPALLARDFPDLDLLDPDIPDVAELERRAKEAEAAALAV
                     KGVSKSGGASASAGIGGMVLVTSAGFHGSYLRSSQGISMTAISGEGTGMERDYDYTSA
                     PHAADLEKPEQVGRRAGERAVARGGPHKVETCKVPVVFDPRVSGSLVGHVVGAVNGAS
                     IARKTSFLKDKLGQQLFAKNIRIIDDPLRKRGLRSQSFDAEGVRVQKLAIVDEGVLTT
                     WLLDSATARELNMKTTGHAHRGVSSPPSPGPYNLHLEAGEVTPAELMSDITQGFYVTD
                     LIGSGVNGVTGGYSRGASGFWIENGKVTYPVSEVTIAGHLLEMFKSLAVANDLEFRYG
                     VNAPTVRIEGLTLGGR"
     gene            3254..3778
                     /locus_tag="VM910_14455"
     CDS             3254..3778
                     /locus_tag="VM910_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HVI63767.1"
                     /translation="MRRQAATSGVNPAGSSRALRLDPLSLPVSFNAYDLRADGGMRQI
                     ELHRERVVLRRAVHGMRMAVQVRITDFLGVALRGGDDGAMLVLKHRDPSLTIPLFVGD
                     DMNEIATKWLMWSELFALPQLQDKEPREPAARRRRRNAIRWRRPKFLMRRRVGDRLGS
                     VKVHRGEREIIARS"
     gene            complement(3775..4707)
                     /gene="ubiA"
                     /locus_tag="VM910_14460"
     CDS             complement(3775..4707)
                     /gene="ubiA"
                     /locus_tag="VM910_14460"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HVI63768.1"
                     /translation="MSDATARVADSTGNWVDTTAPPWSRPYLRLARLDRPIGSWLLLI
                     PCWWSLALAAGVAHEPALLPLNLALFFIGAFAMRGAGCTWNDITDRDLDARVERTRSR
                     PIPAGQVSVKQAFAFLALQALLGLLVLLQFNRFAVLTGIASLLIVAIYPFMKRITWWP
                     QVVLGLAFSWGALMGFAVTFGRLDATALVLYAGSIAWVIGYDTIYAHQDTDDDALIGV
                     KSTARLFGARTQSALIVFYGLAVFLIGIALALAGVGAAAWIGLAAFAAHLAGQIRRLR
                     IGDPALCLKIFKSNRDAGLLLFAGLLIDAVVRAA"
     gene            complement(4710..5399)
                     /locus_tag="VM910_14465"
     CDS             complement(4710..5399)
                     /locus_tag="VM910_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63769.1"
                     /translation="MIRRLIVPLTLAALSVQGGLALAQGAFPAPLPNQTVRNDPAFPP
                     VNGAAPSASIGAPPPSPSFPVNGAAPVAGGSGGGGGFGPPPTAAAAPSGGAGEACMKG
                     FVPLREEAEKRGKLIKAASERHAPPEEACKLIASFSQSELKMINYVETNATKCGIPAQ
                     VAEQLKTGHKNTEGMQKRVCNVAEQIKTRGPGGPSLSEVLGSATSAPEVTTAKKGGST
                     FDTLNGNVLER"
     gene            5640..6395
                     /locus_tag="VM910_14470"
     CDS             5640..6395
                     /locus_tag="VM910_14470"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HVI63770.1"
                     /translation="MPELDFRAPRLFVDASLVPGQVVALERDQSNYLGNVLRLGAGDT
                     ILVFNGEDGEWRASIAGRKRPDSLRIETRTRAQDRLPDLTYAFAPLKHARLDYMVQKA
                     VEMGASLLQPVVTRFTQVSRVNGERMRANVIEAAEQCGILSLAAVADPVPLERFLDAR
                     NQKRLLVFCDEAADVADPFQALRERPAGSKGVDVLVGPEGGFAEDERTLLLRQPQIVK
                     LALGPRVLRADTAAVAALALVQATLGDWIGSAK"
     gene            6532..7719
                     /locus_tag="VM910_14475"
     CDS             6532..7719
                     /locus_tag="VM910_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HVI63771.1"
                     /translation="MTATAAASASAKASASASSGWADALLASFAQAGYVRAEPPILQP
                     AEPFLDLSGEDIRRSLYLTTDATGEELCLRPDLTIPVARDYLASPKAGQPAGFSYLGP
                     VFRYRADRPSEFLQAGIESFGRQDRAAADAEMLALALEATSAFGVGDVEIKTGDVALF
                     NALIDALQLYPVWRRRLIKDFNRRISLTQDIEQLTLKTGPGGNEYEGVLAALAGSDRK
                     AALALVTDLMSIAGASNVGGRTVAEIADRFLEQSTLKAGALPRDAASIIKRFLSISGD
                     PDDAVAQLRKLASDAKLDITAAIDQLESRIGFMAARGIDVARTRFSTSFGRGLDYYTG
                     FEFELHKTGDGVEPLVAGGRYDGLMTQLGAATPIPAVGFSVWVETVTQIARAPAASGG
                     SKP"
     gene            7716..8705
                     /gene="hisG"
                     /locus_tag="VM910_14480"
     CDS             7716..8705
                     /gene="hisG"
                     /locus_tag="VM910_14480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HVI63772.1"
                     /translation="MSTPFVLAVPSKGRLQENAEGFFTHAGLSLAKPRGAREYRGTMV
                     GYDNVEIAYLSASEIASQLASGAVHLGVTGEDLVRETIADAGKRVLLIDQLGFGHANV
                     VVAVPQAWIDVRTMADLDDVTTGFREQHNRRMRVATKYINLTRAFFASHGVVDYRIVE
                     SAGATEGAPAVGTAEMIVDITTTGATLAANGLKVLDDGVILRSQANLVASREADWSPE
                     ALETARIILDHIHSRARARKYREVRTRFAGCNDALLAEAHDRFGVVSPFGGPTSSGML
                     TLHCPPSQLYALGSFLRAHGADTVSVASLDYVLDRENPLFEKLEGFLRDPRQY"
     gene            8822..>9610
                     /locus_tag="VM910_14485"
     CDS             8822..>9610
                     /locus_tag="VM910_14485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI63773.1"
                     /translation="MKLGSDVSSLTTTAANAAVQGLSIVVPVYNEAAGLELLHRRLSA
                     LAARLRQRFGLASEVVYVDDGSADATLAIARGLEADTLDVQIVSLSRNFGKEAALMAG
                     LDHAKRGAVLFMDGDGQHPPDLVEQLVGHWIEGGYDVVYTAKAHRDNESILRRFGVHG
                     FYALINWGARQKIPEDAGDFRLLSPRAAAALRQLPERNRFFKGLASWIGFRQIRVDYE
                     PAPRAHGVTTFNVMRLLGLSIEGLTSFSVAPLRFASLLGVLLASA"
BASE COUNT         1724 a         3166 c         3060 g         1660 t
ORIGIN      
        1 tggtcgccgg agccgcgaat cgtgccgaca ccgagccgct ccgccgcgat cgcgttgtac
       61 tcgccgtccc gatgcagcga aatcaacacc ttggcccgat agcgcttgag cctgatgaag
      121 ggggtgagga aatgctggcc atgccagaag gcgaagatcg ccggcatctg cgcctcgacc
      181 cgggcctgga tattgggcgg atccatggtg atacggttgg tccgccacac cagccgcaga
      241 tactcggccg cgaggaaacc gactgcgccc tgaaaccatt tactgcgcag cgtattgcgg
      301 agcagtttct tcaacgggtt ggtttcgtgg cttgcttggg atcgttgccc ggatcgagca
      361 ggcgatgcag atgcacgacg aaatagcgca tatgcgcatt gtcgacggta gcctgcgccc
      421 tggccttcca ggccgcgtag gccgtcgcgt aattgggaaa tacgccgacg atgtccaccc
      481 gatcgagatc cttgaagatg ttgttttcga gatcggtcaa ttcgcccccg atcacgaggt
      541 gaagcaattg ctgctgcggg ccattgtctg gcatggttgt tttcctcgag gagacgcccg
      601 gcaagcactt ttcgaaacgc atctttcgat gcgcatcaaa tccaaaaaca cacccggtgt
      661 ccagattccg attccgaagt tcgcatcatt gagcgggtcc ttcgattttg cgaactcgcg
      721 gcaatgatga tgctgacttc tgaaccgcca cacccgggtg tcacggcaac ggtcgtttgc
      781 tgaagtgcga gacgatgtcc gcatgacggt cgcgtcccgc tgccaccagc accccgtgcg
      841 tcacctcccg gcgattgtag agaatcgcgt ccccggaaag cgcagtcatg ttaccattcg
      901 cttcctgtac gatcaaattc gcggcggcaa gatcccagtc gcggctctga ccgccggcaa
      961 aggcggcatc gagacttcct tgcgccaccc gacagagtct gagcgccaga gacgcgatgc
     1021 gcggatgcag cacgatctcg ccgcgcgagg gattgagccg ctccaccaga ggttttggcc
     1081 cggccacgcg tgaaaagtcg agctctgtgc ccgaggtggc ctgcagcgcg atatcgttac
     1141 gggttgcgcc ctgcccgcgc gcggcaaaaa agaattcgtc gcttgccggc gcaaacacgg
     1201 cagccagcac cggagctgcc ccctcgacca gcgccgcgct gacgcaccag tcttcgcggc
     1261 cggcgagata gccgcgcgtg ccgtcgatcg gatcgacgat ccagaccagg cgcttggaca
     1321 gccgatgctc gtcgtcggcg ctttcctccg acagccagcc atagtcgggc gtggcggcgc
     1381 gcagccgctg ttcaaggagg tcattgaccg cgatatcggc ttccgagacc ggcgacgacg
     1441 ctcccttggt ccagttcctg agctcggtgc ggaacagcga cagcgcgagc tcgccggcct
     1501 cgcgtacggt atccttcagc aaatcggcgt cgcgcgtcag agcgaatgtt cgaaacggac
     1561 cactagcgtc caccaagcgt caatccctcg atgcgcaccg tcggcgcatt gacgccgtaa
     1621 cggaattcca gatcgttggc caccgccagc gacttgaaca tctccaaaag atgccccgcg
     1681 atcgtcacct cgctcaccgg ataggtgacc ttgccgttct cgatccagaa gccggatgcg
     1741 ccacggctgt agccgccggt cacgccgttg acgccggagc cgatcaggtc ggtgacgtag
     1801 aaaccctgag taatgtcgga catcagttcg gccggcgtca cctcgcctgc ttcgagatgc
     1861 agattgtaag gtcccggcga aggcggcgac gacacgccgc ggtgggcatg gcccgtggtc
     1921 ttcatgttga gctcgcgggc cgtcgccgaa tccaggagcc aagtcgtcag cacgccctcg
     1981 tccacgatcg cgagcttctg caccctgacg ccctcggcgt cgaacgactg cgagcgcagg
     2041 ccgcgcttgc gcaagggatc gtcgatgatg cggatattct tcgcaaacaa ttgctgaccg
     2101 agcttgtctt tcaggaaact cgtcttgcgc gcgatcgacg cgccgttgac ggcgccaacc
     2161 acatggccga ccaacgatcc cgaaacgcgc ggatcgaaca cgacgggcac cttgcaggtc
     2221 tcgaccttgt gcgggccacc tcgcgcaacg gcccgctcgc cggcgcggcg cccgacctgc
     2281 tcgggtttct ccaggtcggc ggcatgcggc gcggacgtat agtcataatc gcgctccatg
     2341 ccggtgccct ctcccgagat tgcagtcatc gagatgccct ggctcgagcg caggtaagaa
     2401 ccgtggaagc cggcgctggt gaccagcacc atgccgccga taccggccga cgccgatgcg
     2461 ccgccggatt tgctgacacc tttgaccgca agcgccgcgg cctcggcctc tttcgcacgc
     2521 cgctccagct cggcaacgtc aggaatatcc ggatcgagca gatcgagatc cggaaaatcg
     2581 cgtgccaata gcgcgggatc ggcaaggccg acatatttgt cgtcgggggc aacgcgggcc
     2641 attgccaccg cgcgctcggc cagtcgcgca atgccgtcgc cgctcacgtc gttggtcgaa
     2701 accaccgcct ggcgctggcc gaccagcacg cgcaggccga catcgtcgcc ctcggagcgc
     2761 tcgctctcct cgacgcgtcc gtcgcgcagc tcgacggcct gcgagacgcc gcgcaccgcc
     2821 acggcatcgg ccgcatcggc gcccgcgcgt ctggcagcct cgaccagccg ctcggcaagc
     2881 gcgctcaggg ccgattgatc gaacaggtcg cgaccggccg atttggcggg aagcggcgat
     2941 acagttgatg gtgaagagat cacggacaaa atccttcgag ccttgaagcg aggcgctgac
     3001 ggaacatcgg caaaatcacc agatgtgccc ggctggtgcg gacttcaagc atttttcgtg
     3061 cggtaaaagc ctaaagattg cacctctccc gcaacgcctc gtcaatcatg cggaccaagg
     3121 tcttgtcaat catgcccata gatgtggggc cggtgagcac tctttaacca ggctttttaa
     3181 gctgaaacgg acgcatcccc gataaggtct cgcgtatcga ccgggaacat aatcccggcg
     3241 gggagacgaa gccgtgaggc gtcaagcagc aacaagcggg gtcaatcccg cggggtcgag
     3301 ccgcgcattg cggctcgacc ccctttctct gcccgtcagc ttcaacgcgt acgacctgcg
     3361 tgccgatggc ggcatgcggc agatcgagct tcaccgcgaa cgtgtcgtgc tgcgtcgtgc
     3421 cgtccatggc atgcggatgg cagtgcaggt tcgcatcacc gattttctgg gcgtagcgct
     3481 gcgcggcggc gacgacgggg cgatgctggt gctcaagcat cgcgaccctt cgctgaccat
     3541 cccgctgttc gtcggcgacg acatgaacga aatcgcaacc aagtggctga tgtggagcga
     3601 gctgtttgcg cttccgcagt tgcaggacaa ggagccgcgc gagcccgcgg cccgacgccg
     3661 gcgccgcaac gcgatccgct ggcggcggcc gaaatttctg atgcggcggc gcgtcggcga
     3721 tcggctggga tctgtcaagg tgcatcgcgg cgagcgtgag atcatcgcgc ggagctaagc
     3781 cgcacggacc acggcatcga tcagcaatcc cgcgaacagc aacagcccgg catcgcggtt
     3841 ggatttgaag atcttcaggc acagcgccgg atcgccgatc ctgagacggc ggatctgacc
     3901 ggcaagatgc gcggcaaagg ccgcgagacc gatccacgcc gcagcaccga cgcccgcaag
     3961 cgcaagcgcg atcccaatca ggaagacggc aagtccatag aagacgatca acgcggactg
     4021 cgtgcgcgcg ccgaacaggc gcgcagtcga cttgacgccg atcagcgcgt cgtcgtcggt
     4081 gtcctgatgc gcgtagatgg tgtcgtagcc gatcacccag gcgatcgagc ccgcatagag
     4141 cacgagcgcg gtggcatcga gccggccgaa tgtcaccgca aagcccatca aggcgcccca
     4201 ggaaaacgcc agccccagca cgacctgcgg ccaccaggtt atgcgcttca tgaacgggta
     4261 gatcgcgacg atcagcagcg aagcgatgcc ggtcagaacc gcgaagcggt tgaattgcag
     4321 cagcaccaga aggccgagca gggcctggag ggcaaggaac gcgaaggcct gcttcacgct
     4381 cacctggcct gcgggaattg gccgcgagcg cgtgcgctcg accctggcat cgagatcgcg
     4441 atcggtgatg tcgttccagg tgcagcccgc gccacgcatg gcgaaggcgc cgatgaaaaa
     4501 tagcgcaagg ttgaggggca gaagcgctgg ctcgtgtgcg acccctgcgg cgagcgcgag
     4561 cgaccaccag cagggaatca gaagcagcca ggatccgatc gggcgatcga gtcgggccaa
     4621 cctgaggtag ggccgcgacc atggcggtgc ggtcgtgtcg acccaattgc cggtcgagtc
     4681 cgcaacccgc gcggtcgcgt cactcatcgc tacctttcga gaacgttgcc gttcagcgtg
     4741 tcgaaggtgc tgccgccctt ctttgcagtc gtgacctcgg gcgccgaggt cgcagaaccg
     4801 agcacctcgc tcaagctcgg gccgcctggc ccccgggtct tgatctgctc ggccacgttg
     4861 cagacccgct tctgcatgcc ctcggtgttc ttgtggcccg tcttcaactg ctccgcgacc
     4921 tgcgccggga ttccgcactt cgtcgcgttg gtctcgacat aattgatcat cttgagttcg
     4981 gactggctga aactggcgat cagtttacag gcctcctccg gcggcgcatg ccgctcgctg
     5041 gccgccttga tcagcttgcc acgcttctcg gcctcttcgc gcagaggtac gaagcccttc
     5101 atgcaggcct ccccagcgcc acccgaaggc gccgcggcgg ccgtcggcgg aggcccgaag
     5161 ccgcctcctc caccgctgcc accggcaacc ggcgccgcgc cgttcaccgg aaatgatggg
     5221 ctcggcggcg gggccccgat ggaggccgac ggggccgcgc cgttgaccgg cggaaatgcg
     5281 ggatcgttcc tgaccgtttg gttaggcagc ggagcaggaa aagcgccttg tgccagcgct
     5341 agcccgccct gaacagacag cgcggccagc gtcaacggca caatcaggcg gcgaatcatc
     5401 aaggctttca tctccggcaa ggtccgacgg cccacgaatg ctccgaatct cattgttcgg
     5461 aaagcggttt tgtccggcgc tcaggcgata gctgttcgta tgctttttac gattcctgcc
     5521 ggttcttaac aacccgtcga ttttggcaac aacgcggcgc agagccgcac cttcacgcca
     5581 ataattgccc ccgattctag acctttaggt taaaaccggg gccctcggaa cgatcacgaa
     5641 tgcctgaatt ggattttcgc gcgcctcggt tgtttgtcga cgcctcgctt gtgcccggac
     5701 aggtggtcgc actcgagcgc gaccaaagta actatcttgg caacgtgctc cggctcggag
     5761 caggcgacac aatcctggtg ttcaacggag aggacggcga atggcgcgcg tccatcgccg
     5821 gccgcaaacg gccggacagc ctccgcatcg agactcggac gcgggcgcag gaccgccttc
     5881 ccgatctcac ttacgccttt gcgccgctca agcacgcacg cctcgactac atggtgcaga
     5941 aagctgtcga gatgggcgcc tcgctcctgc agccggtcgt gacccgtttc acccaggttt
     6001 cgcgggtcaa cggcgaacgg atgcgcgcca atgtcatcga ggcggcggag caatgcggga
     6061 ttttgagcct ggctgcggtg gccgatccgg tcccgctcga acgcttcctg gacgcacgca
     6121 atcagaagcg gctcctggtg ttctgcgacg aggcggctga cgtcgccgat ccgttccagg
     6181 cgctgcggga gcggccggcg gggagtaagg gcgtggacgt cctggtcggc cccgagggcg
     6241 gattcgcgga agacgagcgc accctcctgt tgcggcagcc gcaaatcgtg aagcttgccc
     6301 tgggtccgag ggtcctgcgg gccgatactg cggccgttgc cgcgctcgcg ctggtccagg
     6361 ccacgctcgg cgactggatc ggctcggcca aatagttaaa tcgacgtcta aacctgttaa
     6421 gccacttgtc cgatccctgt cgaaactgcg agccggccat gctaaggggc tgcgccaaca
     6481 agggcttttc gacctttgaa ccggataccg gacgttaaga gacgttagga catgaccgca
     6541 accgccgcag cgagcgcttc tgccaaagct tccgccagcg cctcgtccgg ctgggcggac
     6601 gcgctgctgg cttcgttcgc gcaggccggt tatgtccgcg ccgagccccc gatcctccag
     6661 ccggccgagc ccttcctcga cctctccggc gaggacatcc gccgaagcct ctacctgacg
     6721 acggatgcca cgggcgagga actctgcctg cgtccggacc ttaccattcc cgtggcgcgc
     6781 gattacctgg cctcaccgaa ggccggccag ccggcgggtt tttcctatct cggcccggtg
     6841 ttccgctatc gcgccgaccg gccaagcgag ttcctgcagg ccggtatcga atccttcggc
     6901 cggcaggacc gcgccgcagc cgacgccgag atgctggcgc ttgcgctgga ggcgacatcc
     6961 gccttcggcg tcggcgacgt cgagatcaag accggcgacg tggcgctgtt caacgcgctg
     7021 atcgacgcgc tgcagctcta tccggtctgg cggcggcggc tgatcaagga cttcaaccgc
     7081 aggatcagcc tgacgcagga tatcgagcag ctcacgctga aaaccgggcc cggcggcaac
     7141 gaatatgagg gcgtgctggc ggcgctcgcc ggcagcgacc gcaaggcagc gctggccctc
     7201 gtcacggacc tgatgtcgat tgccggcgcc agcaatgtcg gcggacgtac ggttgcggaa
     7261 atcgccgacc gcttccttga acagtcgacc ctgaaggctg gcgcgctgcc gcgcgacgcg
     7321 gctagtatca tcaaacggtt tttgtcgatc tcgggcgatc cggatgacgc ggtcgcgcag
     7381 cttcgcaagc tcgcatccga cgcaaagctc gacatcacgg ccgcgatcga tcagttggaa
     7441 agccggatcg gattcatggc cgcgcgcggc atcgacgtcg ccaggacgcg tttttccacc
     7501 tcgttcgggc gcgggctcga ctactatacg ggcttcgagt tcgagctgca caagaccggc
     7561 gacggcgtcg agccgctggt cgcaggaggc cgctacgacg gcttgatgac gcagcttggc
     7621 gcggcgacgc cgatcccggc ggtcggcttt tccgtgtggg tcgagacggt gacccagatt
     7681 gctcgcgcgc ctgcggcaag cggcgggagc aagccatgag cacgccgttc gttcttgcgg
     7741 tcccctcgaa ggggcgcctg caggaaaacg ccgaagggtt tttcacccat gccgggcttt
     7801 cgctcgccaa accgcgtggc gcccgcgagt atcgcggcac gatggtgggc tacgacaatg
     7861 tcgagatcgc ctatctttcc gcaagcgaaa tcgcttcgca gcttgcgagc ggcgcagttc
     7921 atctcggcgt caccggcgag gacctggtac gcgagaccat cgccgacgcc ggcaaacgcg
     7981 tgctcctgat cgaccagctc ggctttggcc acgccaatgt ggtggtcgca gtcccgcagg
     8041 cctggatcga cgtccgcacc atggccgatc tcgacgacgt cacgaccggc ttccgcgagc
     8101 agcataatcg cagaatgcgg gtcgcgacca agtacatcaa tctgacgcgc gcattctttg
     8161 cctcccacgg cgtggtcgac taccgcatcg tcgagagcgc cggcgcgacc gaaggagcgc
     8221 cggcggtcgg gacggccgag atgatcgtcg acatcacgac gacgggcgca acgctggccg
     8281 ccaacgggct gaaagtgctg gacgacggcg tcatcctgcg cagccaggcc aacctcgtgg
     8341 cctcgcgcga ggccgactgg tcgccggagg cgctcgagac cgcgcgcatc atcctcgacc
     8401 acatccactc gcgcgcccgc gcgcgcaaat atcgcgaggt gcgcacgcgc tttgccggat
     8461 gcaacgatgc gctgctggca gaggcccatg atcgcttcgg cgtggtttcg ccattcggcg
     8521 gcccgacgtc ctcgggcatg ctgacgctgc actgcccgcc cagccagctc tacgcactcg
     8581 gcagcttcct gcgcgcgcac ggcgccgaca ccgtctcggt tgcgtccctc gactatgtgc
     8641 tcgaccgcga gaacccgcta tttgaaaagc tcgagggctt cctgcgggac ccgcggcaat
     8701 actgaagcga atgtccaatc cgccgcgccg gatggtggcg acaagcccca tcgattgcca
     8761 tatggtccga gcgggaaacc tgttctgacc gggaaaccgg gacgccggaa aaagcgatgc
     8821 gatgaagctg ggtagcgatg tctcaagcct gactaccact gcggccaacg ccgcggtgca
     8881 gggtctgtcg atcgtcgtcc ccgtctataa cgaggcggcc ggattggagt tgctgcaccg
     8941 tcgtttgagc gcgctggcgg cgaggctgcg gcagcgcttc gggctggcaa gcgaggtcgt
     9001 ctatgtcgac gacggctccg cagatgccac gcttgcgatc gcgcgcggtc tggaggcgga
     9061 cacgctggat gtgcagatcg tctcgctgtc gcgcaatttc ggcaaggagg cagccctgat
     9121 ggccggcctc gatcatgcca aacgcggcgc cgtgttgttc atggacggtg acggccagca
     9181 cccgccggat ctcgtcgagc agctcgtcgg ccactggatc gaaggcggct atgacgtcgt
     9241 ctacactgcc aaagcgcatc gcgacaatga gtcgatcctg cgccggttcg gcgtgcacgg
     9301 cttctacgcg ctgatcaact ggggggcgcg ccaaaaaatc ccggaagatg ccggggactt
     9361 ccgcctcttg tcgccgcgcg cggctgcggc gctcaggcag ttgcccgagc gcaaccgctt
     9421 cttcaagggt cttgcgagct ggatcggctt tcgccagatc cgcgtcgact acgagccggc
     9481 gccgcgcgcc cacggcgtca ccactttcaa cgtgatgcgg ctcctcggcc tttcgatcga
     9541 gggactgacc tcgttctcgg tggcgccgtt gcgctttgcg agcctgctcg gcgtgctcct
     9601 cgcgtccgcc
//
LOCUS       DATLJT010000625         5451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_725847,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000625 DATLJT010000000
VERSION     DATLJT010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725847"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VM910_14490"
     CDS             complement(<1..666)
                     /locus_tag="VM910_14490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI63774.1"
                     /translation="MLARDWYYNERRRIGLDSTIASIYDRRDNSDLRAREALSMLGVR
                     AGWRVADIGCGNGVLATEAALMGAEVDAVDISPAMLALAEVYARDRRAVIRAQPAGLL
                     SFAYEPNSYDLIVSEFTLHHLPDFWKAVALARIFAALKPGASFYLRDIVFVSMPDGSE
                     RDVDEWADFNIKNHDFERDSVVTHMRDEYSTFGWVIERMLTEVGFILVSADYHAPLHG
                     TYLL"
     gene            complement(669..1211)
                     /gene="ilvN"
                     /locus_tag="VM910_14495"
     CDS             complement(669..1211)
                     /gene="ilvN"
                     /locus_tag="VM910_14495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HVI63775.1"
                     /translation="MSQPASAYFIEDRHDPNETHTLSVLVQNEPGVLARVIGLFSGRG
                     YNIESLTVSETESQKHLSRITIVTTGTPMVIEQIKHQLDRMVPVYRVVDMTLTGRSIE
                     RELAMVKVRGSGEHRVEALRLADAFRARVIDATSESFVFEITGNTAKINQFIDLMRPL
                     GLIEVSRTGVAAIGRGPEGM"
     gene            complement(1291..2274)
                     /locus_tag="VM910_14500"
     CDS             complement(1291..2274)
                     /locus_tag="VM910_14500"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HVI63776.1"
                     /translation="MFDRGELERALEVVHAAMPPTPAHAWPLLAERLGTPVVVKHENH
                     TPIGAFKVRGGLVYLERLKRERPNTPGIISATRGNHGQSLAFAASRHGVPAVIYVPRG
                     NSVEKNRAMKGFGAELVEHGEDFQAAREEAERRAQFAGLHMVPSFHPDLVLGVATYAL
                     ELLRRHGDLDVLYVPIGQGSGICGCILVRDLLGLRTEIVGVQSTLAPSYALSFAAGAV
                     VTTETSNTLADGMATRIPDQDALSIIRKGASRIVQVTDEEVASAMRAYWTDTHNLAEG
                     AGAAALAAALQERSRLRGKRVALILSGGNIDFDLFRRWVMADNAVAERMAV"
     gene            complement(2285..4060)
                     /locus_tag="VM910_14505"
     CDS             complement(2285..4060)
                     /locus_tag="VM910_14505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HVI63777.1"
                     /translation="MSKTSHDPNQMTGAAMIVRALVDHGVKHIFGYPGGAVLPIYDEI
                     FQQSDIEHILVRHEQGAGHAAEGYARSTGKTGVVLVTSGPGATNMVTPLTDALMDSIP
                     LVCITGQVPTHLIGNDAFQECDTVGITRPSTKHNWLVRNVNDLAKVLHEAFYVATSGR
                     PGPVVVDVPKDVQFAVGTYHPPRKSDVHVSYAPRVKGDAGQIRKAVALLAAARRPVIY
                     SGGGVINSGREASKLLRELVEVTGFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANM
                     AMHDCDVMLCVGARFDDRITGRTDAFSPGSKKIHIDIDPSSINKNIRVDVPIIGDVAN
                     VLGDLLQVFKSEAKKPDVKAWWAEIAKWRARNSLYYKKNNEVILPQYAIQSLFEATRG
                     KDVYITTEVGQHQMWAAQFFGFEEPHRWMTSGGLGTMGYGLPSAVGVQVAHPDSLVID
                     IAGDASVQMTIQEMSTAVQYELPIKIFILNNQYMGMVRQWQQLLHGNRLSHSYTEALP
                     DFVKLAEAYGCVGLRAVKPADLDGAIKEMISVKRPVLFDCRVAAMENCFPMIPSGKAH
                     NEMLLPEQATDDATASAFAGGKALV"
     gene            complement(4477..5406)
                     /gene="miaA"
                     /locus_tag="VM910_14510"
     CDS             complement(4477..5406)
                     /gene="miaA"
                     /locus_tag="VM910_14510"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HVI63778.1"
                     /translation="MTSQEPGIKAVLIAGPTASGKSALALELAEKRGGVVINADSMQV
                     YRDLRVLTARPTAAEEARVPHRLYGHVDAAVNYSAGHFLADAAAVLAEAREQGRLPIF
                     VGGTGLYFKALTQGLSAVPPIPPDVREAVRARLDHDGVEALHAELSRRDPLSAARLKP
                     RDRTRIARALEVVEATGRSITDWHREGLPPLLPTGAFHALFLAPERDALYQRIDARFD
                     AMLTGGALPEVGRLAGRRLDPLLPAVKAHGVRPLIRHLKGEITLAEATAIGRADTRHY
                     AKRQFTWFRHQLPEFDWVKPEAVRGWLEEKSRP"
BASE COUNT          971 a         1773 c         1706 g         1001 t
ORIGIN      
        1 caagagatac gtgccgtgaa ggggagcgtg ataatccgcc gacaccagga taaagccgac
       61 ttcggtcagc atgcgctcga tcacccagcc gaaggtggaa tattcgtcgc gcatgtgggt
      121 gacgacgctg tcgcgctcga aatcgtggtt cttgatattg aagtccgccc actcgtccac
      181 gtcgcgctcg gagccgtcgg gcatgctgac gaacacgatg tcgcgcagat agaagctggc
      241 gccgggcttg agcgcggcaa agatccgcgc cagcgccacc gccttccaga aatccggcag
      301 atgatgcagc gtgaactcgc tcacgatcaa atcataggaa ttgggttcgt aggcgaaact
      361 gagcagcccc gccggctgcg cgcgaatgac cgccctgcgg tcgcgggcat agacctcggc
      421 aagcgccagc atcgcgggcg agatatcgac ggcgtcgacc tcggcgccca tcagcgcggc
      481 ctcggtcgcc agcaccccgt tgccgcagcc gatatcggcc acgcgccatc ccgctcgcac
      541 gccgagcatg ctcagcgctt cgcgggcgcg gaggtcactg ttgtcgcggc gatcgtagat
      601 cgaggcgatc gtactatcga gtccgatccg ccggcgctcg ttgtaatacc agtcgcgcgc
      661 cagcatggtc acatcccctc tggcccgcgt ccgatcgcgg cgacgccggt tcgggagact
      721 tcgatcaggc cgagcgggcg catcaggtcg atgaactggt tgatcttggc cgtgttgccg
      781 gtgatctcga acacgaagct ttcgctggtg gcgtcgatca cccgcgcgcg aaatgcgtcc
      841 gcaagacgca gggcctcgac gcgatgctcg cccgagccgc gcaccttcac catggcgagc
      901 tcccgttcga tcgagcgccc ggtcagtgtc atgtcgacca cgcggtagac cgggaccatg
      961 cggtcgagct gatgcttgat ctgctcgatc accatgggcg tccccgtcgt gacgatggtg
     1021 atgcgggaaa gatgtttctg gctctcggtc tccgagaccg taagactttc gatgttgtag
     1081 ccgcggccgg agaacagccc gatcacgcgc gcgagcacgc cgggctcgtt ctggaccaga
     1141 acggagagcg tatgcgtctc gttcggatcg tggcgatctt cgatgaaata ggccgatgca
     1201 ggctggctca ttgtcgtccc ccgatcatct ccgtcgtcat gggaaaggcc cggccatgac
     1261 ggctgtatct gtgatgcgat gactgcctca tcacactgcc attctttctg cgacggcgtt
     1321 gtccgccatc acccatctcc ggaacaaatc gaaatcgatg ttgccgccgg acaggatcag
     1381 cgcgacgcgc ttgccgcgaa gtctgctccg ctcctgcagc gcggccgcga gcgccgcagc
     1441 gccggcgcct tcggcgagat tgtgcgtgtc ggtccagtag gcgcgcatgg cagaggcaac
     1501 ctcttcgtcg gtgacctgca cgatgcggga ggcgcccttg cggatgatcg agagcgcatc
     1561 ctgatccggg atgcgggtgg ccatgccgtc agcgagcgtg ttggaggttt cggtcgtcac
     1621 gaccgcgccg gccgcgaacg acaacgcgta ggatggtgca agcgtcgact gcacgccgac
     1681 gatctcggtg cgaagaccca ggagatcgcg cacgagaata catccgcaaa tgccggaacc
     1741 ctgcccgatc ggcacgtaga gtacgtcgag gtcgccatgc ctgcgcagca attccagcgc
     1801 gtaggtcgca actcccagca ccagatccgg gtggaacgac ggcaccatat ggaggcctgc
     1861 gaattgcgcg cggcgttcgg cctcttcgcg cgcggcctgg aaatcctcgc cgtgctcgac
     1921 caattcggcg ccaaaacctt tcatcgcgcg gttcttctcg accgaattgc cgcgcggcac
     1981 gtagatcacc gccggcacgc cgtgtcggct cgccgcaaac gcgaggctct ggccatgatt
     2041 gccgcgcgtg gccgaaataa tgcccggcgt gttcggccgc tcgcgcttca gccgctcgag
     2101 atagaccaag ccgccgcgca ccttgaaggc tccgatgggc gtgtggttct catgcttgac
     2161 gacgacaggc gtaccgagcc gttcggccag gagcggccag gcatgcgccg gcgtcggcgg
     2221 catcgccgcg tggacgacct ccagcgcccg ttcgagttcc ccgcgatcga acatcggctg
     2281 cccctcacac caacgccttg ccgccggcaa aggcggatgc ggtcgcgtcg tcggttgcct
     2341 gctccggcag cagcatctca ttgtgcgcct tgcccgacgg aatcatcggg aaacagtttt
     2401 ccatcgccgc cacgcggcaa tcgaacagca ccgggcgctt caccgaaatc atctccttga
     2461 tcgcgccgtc gagatcggcg ggtttcaccg ccctcagtcc gacgcaaccg taggcttccg
     2521 caagcttgac gaaatccggc aaggcttcgg tgtaggagtg cgacagccgg ttgccgtgca
     2581 gaagctgctg ccactgccgc accatgccca tgtactggtt gttcaggatg aagatcttga
     2641 tcggcagctc gtactgaacc gcagtcgaca tctcctgaat cgtcatctgc accgaggcgt
     2701 cgcctgcaat gtcgatgacg aggctgtcgg gatgggcgac ctgcacgccg accgccgacg
     2761 gcaggccgta acccatggtg ccgagcccgc cagaggtcat ccagcggtgc ggctcttcga
     2821 aaccgaagaa ctgcgcggcc cacatctgat gctggccgac ctcggtcgtg atgtagacgt
     2881 ccttgccgcg cgtcgcctcg aacaggctct ggatcgcgta ttgcggcagg atgacctcgt
     2941 tgtttttctt gtagtacagc gaattgcgtg cgcgccactt ggcgatctcg gcccaccagg
     3001 ccttgacgtc gggcttcttc gcctccgact tgaacacctg caaaaggtcg cccaacacgt
     3061 tggcgacgtc gccgatgatc ggcacgtcga cccggatgtt cttgttgatc gaggacggat
     3121 cgatatcgat gtggatcttc ttcgaacccg gcgagaacgc gtcggttcgt ccggtgatgc
     3181 ggtcgtcgaa gcgcgcgccg acgcacagca tcacgtcgca gtcgtgcatc gccatgttgg
     3241 cttcgtaggt gccgtgcatg ccgagcatgc cgagccagtt cttgcccgac gccgggtagg
     3301 cgcccagccc catcaatgtg gacgtgatcg gaaaaccggt cacctcgacc agttcgcgca
     3361 gcagtttcga ggcttcacga cccgaattga tgacgccgcc gccggaatag atcaccggcc
     3421 gcctggcggc ggccaggagc gccacggctt tgcggatctg ccccgcatcg cccttcaccc
     3481 gcggcgcgta ggagacatgg acgtcggact tgcgcggcgg atgataggtg ccgaccgcga
     3541 actgcacgtc ctttggcaca tcgaccacga cgggaccggg acggccgctg gttgcgacat
     3601 agaaggcctc atgcagcact tttgcgaggt cgttgacgtt gcgcaccagc cagttgtgct
     3661 tggtggaggg ccgcgtgata cccaccgtgt cgcactcctg gaacgcatca ttgccgatca
     3721 gatgcgttgg cacctggccc gtgatgcaga ccagcggaat ggaatccatc agcgcatcgg
     3781 tcagcggggt caccatgttg gttgcgccgg ggcccgaggt caccagcacg acgcccgtct
     3841 tgccggtcga gcgcgcgtag ccttcggccg cgtggccggc gccctgctcg tggcggacca
     3901 gaatgtgctc gatgtcgctc tgctggaaga tttcgtcata gatcggcagc accgcgccgc
     3961 ccggatagcc gaaaatgtgc ttcacgccgt gatcgacgag cgcgcgcacg atcatcgcgg
     4021 ctcccgtcat ctggttcgga tcgtgacttg tcttgctcat ggctttctcc agatggcgct
     4081 gttgccagcg gcttgttcgt cttggtcttg gtctcggtct ggattcggaa aataaaaaag
     4141 ggcccgaaga ggccccatgc gcaccgcctg aatttggatg gcattcagcc atccccggcg
     4201 atgcgcctgg gtacgaccac aataagcagt ttggtaataa tattacgcat ttgccgggtc
     4261 agacttccca aaggttgcgc gaaccatagc gaccgaaagc tcaaagtcaa gcgaaacggc
     4321 caacaggggt tacgatttga ctactttagc gggcttccgg ccgtttcggc gagagtcaat
     4381 ttttcgcaac tgctctcgaa gcgcgtcatg gccggctttg accccggtca tccctgctgg
     4441 ccgggagcga tcaacgcgga tgcgcgacgg cagggtttaa ggtcttgatt tttcttcaag
     4501 ccatcccctc accgcctccg gctttaccca gtcaaactcc ggcaattgat ggcggaacca
     4561 ggtgaactgc cgcttggcat agtggcgggt atcggcgcgg ccgattgcgg tggcctccgc
     4621 gagcgtgatc tcgcctttga ggtggcggat caagggccgc acgccgtggg ctttcacggc
     4681 gggcaacagt ggatcgagcc gacggcccgc aaggcgcccg acctccggca gcgcgccgcc
     4741 ggtcagcatc gcatcgaaac gcgcatctat ccgctgatag agcgcatctc gctcgggcgc
     4801 gagaaacagt gcgtgaaacg cacccgtcgg cagcagaggc ggaagccctt cccgatgcca
     4861 gtcggtgatc gaccgcccgg tcgcttccac cacttcgagc gcacgggcga tgcgggtgcg
     4921 gtcgcgcggc ttgagccgtg cagccgagag cggatcgcgc cgcgacaact ccgcatgcag
     4981 cgcctccacg ccgtcgtgat cgagccgcgc gcgtaccgcc tcgcgcacat caggtggaat
     5041 cggcggcacg gccgaaagtc cctgcgtcag cgccttgaaa tagagtcccg tcccgccgac
     5101 aaagatcggc agccgccctt gctcgcgcgc ctcggcaagc acggcggccg cgtccgcaag
     5161 gaaatgtccc gcagaatagt tcacggccgc atcgacgtgg ccatagagcc gatgcgggac
     5221 gcgtgcctcc tccgctgccg tcggccgcgc cgtgagcacg cgaagatcac ggtagacctg
     5281 catggaatcc gcattgatca cgacgccgcc gcgcttttcc gccaattcca gcgccagggc
     5341 cgacttgccg cttgcggtcg gccctgcgat aagcacggcc ttgatgcccg gttcttgcga
     5401 agtcacctga tgtctctcgt cgcgaccctg atctgcaatc ccgataaccc c
//
LOCUS       DATLJT010000626         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2795197,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000626 DATLJT010000000
VERSION     DATLJT010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795197"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VM910_14515"
     CDS             complement(<1..535)
                     /locus_tag="VM910_14515"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63779.1"
                     /translation="MPIIGYLNAGSSDSQSANLAVFRQGLADAGYVEGPNVAIEYRWS
                     RDQPDRLPALAAELVGRKVTVLYAVSNAAAYAAKAATSTIPIVFLSGGDPVEIGLVAS
                     LNRPGGNATGVTLFLNELAGKRLELLRETVPNVAVIAFMVNPTNPRAKVNTSELQTAA
                     RVAGQEILLLNVGHESEF"
     gene            complement(607..849)
                     /locus_tag="VM910_14520"
     CDS             complement(607..849)
                     /locus_tag="VM910_14520"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI63780.1"
                     /translation="MFIARHWAELGGLMSYGADFSGMYRRAAEYVAKILKGAKPTDLP
                     VEQATKFELVVNLKTARAIGIVLPTAILLRAEEVIE"
     gene            complement(807..1502)
                     /locus_tag="VM910_14525"
     CDS             complement(807..1502)
                     /locus_tag="VM910_14525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63781.1"
                     /translation="MRRVGVLMSVADAPEGQARLAAFLKGLQQWGWTEGRNVRIDTRW
                     GGGDAERTRKNVAELLALGPDVFLASGDHPVVELQHATRTAPIVFTMVGDPVGAGFVE
                     SLARPGGNITGFVFQQLELAQKQVELLNQAFPDRTRLAMLFDAQSADQFVAAERTAKL
                     LNLQVQSLRLENPPYDFEAAIRGAASGDAQMLLILSSPFFLPYRARIVEGRMRTASLP
                     CSSPGIGPSWAGS"
     gene            complement(1665..>2210)
                     /locus_tag="VM910_14530"
     CDS             complement(1665..>2210)
                     /locus_tag="VM910_14530"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI63782.1"
                     /translation="INAIASGVSGRIAVCYFEWSGVVWQDLIIDWTVIGSLEAAQQFG
                     EKILLAPRSYPERTSISAAIDFASEQFARAPWVSHRRVIDISGDGDNNTGRGVTTARD
                     EAVAKGITINGLAILSDDLRDHHINPRGGLEQYYRRNVIGGLGAFVVVSVGFESFGDT
                     LIKKLTTEIAAPRYPRYARNW"
BASE COUNT          463 a          681 c          640 g          426 t
ORIGIN      
        1 caaactcgct ttcgtggccc acattgagaa gaagaatctc ttgcccggca acgcgggcgg
       61 cagtctgtaa ttcacttgtg tttaccttcg cccgcggatt ggtcgggttc accatgaaag
      121 cgattacggc gacattcggc actgtctcgc gcaacagctc aagtcgcttg ccagccagct
      181 cattgagaaa cagggttacg ccagtagcgt tgccacctgg ccgattaagg ctggcgacga
      241 ggccgatctc gaccggatca ccgccgctga ggaagacgat cggaatggtt gaggttgccg
      301 ccttggccgc atacgcggca gcgttggaga cagcatatag gacggtaacc ttgcgaccca
      361 ccagttcggc cgccaatgcc ggaaggcggt caggttggtc tctcgaccag cgatattcga
      421 tcgccacgtt cgggccttcg acatatccgg catcggctag gccctgccgg aagacagcca
      481 gattcgccga ttgggaatcg gacgagcctg cattcaaata tccgattatc ggcatccgct
      541 gcgcccgcgc cgcgagtggc cacgtggcca ccgcgccgcc gagaagcgag atgaatgtgc
      601 gccgcttcac tcgatcacct cttcggcgcg cagcaggatt gccgtcggaa gcacgatgcc
      661 gatcgctctg gcggttttga ggttgacgac caactcaaac ttcgtggcct gctcgaccgg
      721 caggtcggta ggctttgcgc ccttgagaat cttcgcgaca tactcggctg cccgtcgata
      781 catccccgaa aaatcggcgc catagctcat gagcccgccc aactcggccc aatgcctggc
      841 gatgaacatg gaagggaggc ggtgcgcatt cgcccttcga cgatgcgcgc tctatacggt
      901 aggaagaagg gactggaaag aatgagaagc atttgcgcat ccccggatgc ggccccgcga
      961 attgcagcct cgaagtcgta gggcggattt tctagccgca gcgattgcac ctgcaggttc
     1021 aagagctttg cagtccgctc cgcggcgacg aactggtcgg cagattgggc atcgaacagc
     1081 atggccaatc gagtccggtc gggaaacgcc tggttcagca gctcgacctg cttttgcgcc
     1141 aactccaatt gctgaaagac aaaaccggtg atgttgccgc ccggccgcgc caggctctcg
     1201 acaaagccgg cgccgaccgg atcaccgacc atggtgaaca cgattggcgc tgtgcgggtg
     1261 gcatgctgca gctccaccac cggatggtcg ccgctggcca ggaaaacgtc ggggccaagc
     1321 gcgagcagct ccgcgacgtt tttgcgcgtt cgctcggcat cgccgccgcc ccagcgggtg
     1381 tcgatccgca cattgcggcc ctcggtccat ccccattgct gcaggccctt caggaatgcc
     1441 gccagacggg cctgtccttc cggagcgtcg gccacgctca tgagcacgcc gacgcgccgc
     1501 atgcgctcgc cctgctgcgc gcgcgccgcc agcggccacg ctgctgcgcc gcccagaagc
     1561 gtgatgaact tgcggcgttg taaccaaatc gtgtgaatcc aaggaggcta cttagagcgc
     1621 gcagcgctgc cacccgaatc tgaatagcca ttccggagaa ggagtcacca gtttctagcg
     1681 taacgcgggt accttggggc tgcaatttcg gtcgttagtt tcttgatcaa tgtatcgccg
     1741 aaagactcga agccaacaga tactacgaca aaagcgccta gaccaccaat gacattgcgg
     1801 cggtagtatt gctcaagacc gccacgagga ttgatatggt ggtcgcgtag gtcgtcactt
     1861 aaaatggcga gaccgttgat cgtgataccc ttcgcgaccg cttcgtccct tgcggttgtc
     1921 acgccccgcc cggtattatt atcgccatct cctgaaatat cgatgacacg gcgatgggat
     1981 acccaaggtg cccgcgcgaa ctgctcgctg gcaaagtcga tagcagcact tattgaagta
     2041 cgctcgggat acgaacgtgg cgctaacaaa attttctcac cgaactgctg tgcagcctct
     2101 aaagacccaa tgacagtcca atcgattatt agatcttgcc agaccacccc tgaccactcg
     2161 aaataacaca ctgcgatgcg gccgctcaca ccggaggcga tagcgttgat
//
LOCUS       DATLJT010000627         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_37835,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000627 DATLJT010000000
VERSION     DATLJT010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37835"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1003
                     /locus_tag="VM910_14535"
     CDS             200..1003
                     /locus_tag="VM910_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVI63783.1"
                     /translation="MDAKTQQQYAETFHGLHRKGDPLILFNVWDVATAEAIAKTSPAV
                     ATSSAAVASALGYADGENVPSDMVTGLVSRMTAAVSVPVSIDLEAGYGDTPDAAAGSV
                     TQILKAGAIGINIEDGLFGGKRQLVSPEQHAAKIKAVRNTAQELGIHLFINARTDPFL
                     LKCGSPDECLNEAARRATAYADAGADGIFVPGLTDLALIEKFTQLTPLPINVMVTQGV
                     PEIPDLARVGVRRVSLGPWPMMAAMRVIGQAAAAVAASKQYGTFLRPNA"
     gene            1029..1439
                     /locus_tag="VM910_14540"
     CDS             1029..1439
                     /locus_tag="VM910_14540"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI63784.1"
                     /translation="MNLPQTGGCLCRKIRYEITETPQLVYTCHCTACQHQTSSAFSLG
                     VAVPETGFRLTSGEPRALQRVTDSGRLNSRFVCPDCASWLYSQPRGGVVRVRAGSLDD
                     TSWLRPTRHLWTRSKQPWVTFAEGDEIFETQPIE"
     gene            1459..1656
                     /locus_tag="VM910_14545"
     CDS             1459..1656
                     /locus_tag="VM910_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63785.1"
                     /translation="MAPEMDIWRTAPLLIRQHGEDAEIVAPQRVDALWQREGYEGHAI
                     WLRVKRALVELQVAPPRISSP"
     gene            complement(1860..2671)
                     /locus_tag="VM910_14550"
                     /pseudo
     CDS             complement(1860..2671)
                     /locus_tag="VM910_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427384.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          572 a          928 c          916 g          536 t
ORIGIN      
        1 cctagccgaa ccaggagacc gtaacggtca aaggctttca ctttctgcag gaggattcgc
       61 ccgacgagta tggccaggcg gtcgcgcggt tcgtcgccaa ggttcgggcc ggagcattgg
      121 ggtgactgaa cgcatatggc ggacgctgga ccggcgttgc tacaacatct ttcgggtttc
      181 tctgaccagg aggcgcgcga tggacgccaa gacgcagcaa caatacgccg agacgtttca
      241 cggtcttcac aggaaggggg atccgctcat cctgttcaac gtctgggacg tcgccaccgc
      301 cgaggccatc gcaaagacgt ctccggctgt tgcgaccagt agcgctgcgg tcgcgtccgc
      361 gcttggatac gctgacgggg aaaatgtccc gtcggatatg gtcacaggtc tggtgtcgcg
      421 gatgaccgcg gctgtgtcgg ttccggtatc aatcgatctg gaggcaggtt atggcgacac
      481 gccggacgcc gccgcggggt cggtcaccca gattctgaag gccggcgcga tagggatcaa
      541 tatcgaggac gggctcttcg gcggaaagcg gcaactcgtc agtccggaac agcatgcagc
      601 gaagatcaaa gcagtgagga acaccgcaca ggaactcggc attcatctgt tcatcaatgc
      661 gagaactgat ccgtttctgc tgaaatgcgg gtcgccggat gagtgcctaa atgaagcggc
      721 aaggcgtgca acggcttatg ccgatgccgg tgccgacgga atttttgtac ccggtctcac
      781 cgatctcgct ctcatcgaga aattcactca actcacgcct ctgccgatca acgtcatggt
      841 gacgcaaggt gttccggaaa tcccggatct cgcgcgcgtc ggcgttcggc gggtgagcct
      901 tgggccgtgg cccatgatgg cggcgatgcg tgtcattgga caggccgccg ccgcggtcgc
      961 cgccagcaag caatacggca cgtttctgcg accgaatgcc tgagcacgtc ctggggagaa
     1021 aaacccacat gaatctgcca cagaccggcg gctgcctgtg ccgcaaaatc cgctacgaga
     1081 tcaccgaaac cccgcaactc gtctatacat gccactgcac cgcctgccag caccagacga
     1141 gcagcgcctt ctcactgggg gtcgccgtgc cggagacggg cttccgcctt acttcgggtg
     1201 aaccccgagc actccaacgc gtcacggata gcggtcggct gaacagccgg ttcgtgtgcc
     1261 cggattgtgc aagttggctg tatagccagc cgcgcggcgg ggtagtgcgt gtgcgcgccg
     1321 gcagcctcga cgatacgtcg tggctgcggc cgacgcggca tctttggacg cgcagcaagc
     1381 agccctgggt caccttcgcc gagggtgacg agatattcga aacccaaccg atcgagtgac
     1441 gcgggcccga tagactcgat ggctccggag atggacatct ggcgcaccgc gcccttgctg
     1501 atccgtcagc atggcgagga tgcggagatt gtcgcgccgc agcgagtaga cgcgttgtgg
     1561 cagcgcgagg gttacgaagg ccacgcgatt tggcttcggg tcaagcgcgc gcttgtggag
     1621 cttcaggtgg cgccaccgcg aatttcctct ccgtagagcc ggacaaggaa gagtgcatca
     1681 gggcacgtaa ggacaagatc acagcaagcc ccaggctccg gttcagcctg caccagtgca
     1741 gacgcggatc gtctgctatc ggcgcaccta cgtcattcct ggtcgctccg gcgacggtcg
     1801 gctcactgat ccgacagcag acgttgggcc ggcgccatca taccgaccgg cgtcaaggcc
     1861 tacgccctgg cgagccgccg gagggcaagc tgggtcgaag caagggtgac aaaggtggcg
     1921 agggtttcgg cgaggttctc gaaatccttg acaagccgcc ggttgcgccc gaaccacgag
     1981 aaggtgcgct cgaccaccca gcgtcgcggc aggacgacga aaccctttat gctgtcgctc
     2041 cgtttgacga tctccaggcg aagccgaggc actttcgcca cgcagcgtca acctgccatg
     2101 cgttgtagcc gccatcggcg cagatcaatt cgagccacgc gaaccgtcgg cgtatcttgt
     2161 cgaggacgaa accggcgccg tcgcggtcct ggatcgccgc cgaatggacg acgacccgca
     2221 tcggcagccc ttcgcagtcg accagggcgt ggatcttgcg tcccttaacc ttcttgccgg
     2281 cgtcgtaacc cacctcgccg tctttgccgc cccctttttc cgctgatttg accgactggc
     2341 tgtcgagaac cgcagccgat gggctggcct cgcgtcccat ccgtccgcgc aaggccatgt
     2401 gcaactcagc ccagatcgcc tctcagacgc catcacactg gaacttgcga aagatgttgt
     2461 agaccgtcga gcgcggcgga aagctctctc gcggcaggta gcgccagggg cagccggtgc
     2521 gcagcagata gaggatagcg ttcaccgccg ctcgcatgtc ggtcttgcgc ggtcgtccgc
     2581 cgggccgcgc cggcgggatc agcggctcca gccgcgccca ctccgcgtcc cgcagatcgc
     2641 tcggataccc gctgccctcg cgtcgataaa ttcgacggtg ctcctcggaa tcatgccatc
     2701 cgaggcgaca ctgcggactt ttgggtcaag ctctaagcga tgacgatctg cacgccgtct
     2761 cggatatcat ctcaaaattg ccagcgccaa atcctatttc tgacacacca gatcccgtcc
     2821 gcatcgagag aggacgggcc cttgcgcagc acttatgcga ccacgctacg aatgccttaa
     2881 aagagttccg gctattgtcg gccttgtgca tcttatgcga ccacgctacg aatgccttaa
     2941 aagagttccg gc
//
LOCUS       DATLJT010000628         1624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_497450,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000628 DATLJT010000000
VERSION     DATLJT010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497450"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VM910_14555"
     CDS             <1..412
                     /locus_tag="VM910_14555"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HVI63786.1"
                     /translation="FLPRDPSVYGNLLRELIAKHNVDCWLVNTGWTGGKYGTGRRMPI
                     KVTRALLTAALNGSLRNADFRTDRYFGFAVPTSLPGVEPHILDPIKTWKDKAEFDKTA
                     RALVAMFQKNFAKFEASVDADIRAAAPEVKLAAE"
     gene            584..943
                     /locus_tag="VM910_14560"
     CDS             584..943
                     /locus_tag="VM910_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63787.1"
                     /translation="MSDIMSVLQNAYATTPLTPTTTRYTPVDPASYQGTWTGTYSNNQ
                     KFEFTISEVNGFRAQVKYASGGTVQYQQVLIGNSSFRIGNTKFVLSGNGTATVGNAVT
                     DPVTGDTSLVQGTAKQS"
     gene            complement(947..>1624)
                     /locus_tag="VM910_14565"
     CDS             complement(947..>1624)
                     /locus_tag="VM910_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVI63788.1"
                     /translation="ALIEHPERSGDPVALTVNLCAPIAEGDFDLDVRLVKANRSSQHW
                     YVELSQGGGDVATLATAIFAERRPSWSHQVALFPESRPFEELKSYAYAPATWVKQYDL
                     RFIEGYPTARANPADPAGSAFSKLWISGRVPRRIDHLSLMAMSDAFFARIFHARGEML
                     PFGTVSITTYFHADSDDLLKEDVTRVLALADAQTFHKSYGDQSGQLWSPSGRLLATTT
                     QMMYFKA"
BASE COUNT          312 a          514 c          509 g          289 t
ORIGIN      
        1 gttcctgccg cgtgacccct ccgtctacgg caatttgctg cgcgaattga tcgcgaagca
       61 caacgtcgat tgctggctgg tcaataccgg ctggaccggc ggcaaatacg gcaccggacg
      121 ccgcatgccg atcaaggtga cacgggcgct tctcaccgcg gcgctgaacg gctcgctgcg
      181 caacgccgat ttccgcaccg accgctattt cggattcgcg gtgccgacct cgctgcccgg
      241 cgtcgagccc catatcctcg atccgatcaa gacctggaag gacaaggccg agttcgacaa
      301 gaccgcgcgc gcgctggtcg cgatgttcca gaagaatttt gccaagttcg aagccagcgt
      361 cgacgccgac atccgcgctg cggcgcccga ggtcaagctc gcggcggagt gacgcgaggg
      421 tctggctccg ggtttctggt ccttcggacg atcccgggat ggcagctaca acaaagggcg
      481 ggtccgtgcg gcctgccctt tttgtcgccg ggttgccctt aacccgccat taaccgtccc
      541 ggtcttctag ttggcctcgc gaccaccagc gcggagcggc agcatgtccg acatcatgag
      601 cgtcttgcaa aatgcctatg caacgacgcc gctcacgccg accactacca gatacacccc
      661 cgtcgatccc gcgagctatc agggcacctg gacggggacc tattcgaaca atcagaaatt
      721 cgagtttacg atttccgagg tcaacggctt ccgcgcccag gtcaaatatg cgagcggcgg
      781 gaccgtgcag tatcagcagg tcctgatcgg caattcctcg ttccgcatcg gcaacaccaa
      841 gttcgtgttg agcgggaacg gcacggcgac cgtcggcaac gcggtgacgg atcccgtcac
      901 cggcgacacc agcctcgtcc agggcacggc caagcagagc tagttttcaa gccttgaaat
      961 acatcatctg cgtcgtggtc gcgagcaggc gtcctgatgg cgaccacaat tgcccggact
     1021 gatcgccgta gcttttgtgg aacgtctgcg catcggcgag cgcgagcacg cgggtgacgt
     1081 cttctttcag gaggtcgtcg gaatcggcgt ggaagtaggt cgtgatcgag accgtaccga
     1141 acggcagcat ctcgccgcgc gcgtgaaaga tgcgggcgaa aaacgcgtcc gacatcgcca
     1201 tcagcgagag atggtcgatc ctccgcggca cgcggccgga gatccacagt ttggagaatg
     1261 cgctgcctgc cggatcagcc gggttcgcgc gcgcggtcgg atagccttcg atgaagcgga
     1321 gatcgtactg cttgacccag gttgccgggg cataggcata ggacttgagc tcttcgaaag
     1381 gcctgctctc cggaaagagc gccacctgat gcgaccatga cgggcggcgc tcggcgaata
     1441 tggcggtcgc cagcgtcgcg acgtcgccgc cgccctggct caactcgaca taccaatgtt
     1501 gcgacgagcg gttggctttg acgagacgga cgtcaaggtc gaaatcgcct tcggcgatcg
     1561 gcgcgcaaag attgacggtg agcgccaccg gatcgcccga gcgctccggg tgctcgatca
     1621 gcgc
//
LOCUS       DATLJT010000629         4792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2795397,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000629 DATLJT010000000
VERSION     DATLJT010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795397"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VM910_14570"
     CDS             <1..992
                     /locus_tag="VM910_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63789.1"
                     /translation="GRSQAAREAERAGPVQVPSRVKLDDGGDPAVILDERTRKRSGVE
                     VIIALPIRYQEQVRAYGTVMDLDNLTTLHNRYVTAVAQIQSAQAKLVASKAAFERAQD
                     LLKSRSMALAQLQAAEAAFGVDQAALATAQAQVKTIRATAFEEWGPAIGKALVEGDAL
                     VSRLIEREAFLIQITLRPGISIDPPPTATVQLSDSVPRVEVRFLSVASQADPKIQGFS
                     FYYVADAESRLLPGMSVLAALPVGASLDGVVVPASAIVWWGGRTWVYLRTGAETFKRH
                     PIPTDLPATDAGGVVVPVAVFPRSPPDLVTRGAQALLSEELRAQIQMGGNDLK"
     gene            989..4195
                     /locus_tag="VM910_14575"
     CDS             989..4195
                     /locus_tag="VM910_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI63790.1"
                     /translation="MRSSGRSPSSASGLQAALIRFAIRFRGVVVALAVVLLGYGVLSV
                     TRAKYDVFPEFAPPQVSIQTEGPGLTPEQIETLVTQPIENAINGVPGVQTLRSSSIQG
                     LSVVTVIFDPASDVYRDRQLVAERLAIAARQFPTGTQPPAMTPLTSSTSTVLVLGLTS
                     DKRSLMDLRTIADWTLRPRLLAVPGITKVSVFGGEVRSIQVAVHPDELIRYNLGFNEV
                     LGAARKATGIRGAGFIDTANQRIVFQSEGQSLKADDIARTVLVNEGALSLRLANVADV
                     VDAPEPPIGGAAIMGQPGVILVVSAQYGANTLEVTRLVENTLADLRRGLEEDGVALHA
                     DLFRPADFVETATTNVRDSLFLGGILVVIVLFLFLFDLRTAAICCTAIPLSLLTAVIV
                     LTAFGVTLNTMTLGGLAIAVGEVVDDAVIDVENIVRRLRQNRQDPSPRSTASVVLDAS
                     LEVRGAVVYASFAVILVFLPVITLPGIAGRLFAPLGFAYVLAILASLLVALTVTPALC
                     MILLAKKARESNDAVVRDRDPPVVNWTRSHYKHLLRGIVAHPKSAIAVAALVTLAGSA
                     VLPFFGAAFLPELKEGHFTLHMSAVAGTSIAESQRLGGLVTGALTELPMVRSVAQRIG
                     RAEKAEDTWGTHYSEFEVALKAGLSGDEIEQAQADLRKALTQFPGVNFALKTFLTERI
                     EEILSGYTASVVVNIFGNDLDLLDLVARDVARVIGGFYGATEVQLQSRPGMPQLTIRL
                     RKQDLERWGFDVIDVLDVIRAAYQGDVVGQTYEGNRVFNVIVTLDPQNRNDIMKVGNL
                     SMRTPGGTYILLSQIADIYQSAGRYQVAHLGAQRLQTVTANVAGRDVAAFVQDAKAVL
                     AGNVELPSGVYVEFAGSAEAQAQSRRDLFFLSGIAGVAIVLLLSIVTRNWRNLVLVLV
                     NMPFALVGGVLAVFATGGQLTLGAMVGFVTLFGITLRNSIMMVSHYEHLVEVEGLHWG
                     VEAAIEGAGDRLTPILMTSLVTALGLLPLAVGMDDPGREVQGPMALVVLGGLLTSMAL
                     NLLILPALALHFGRFERADDEFDQPSPEATRKYHVASE"
BASE COUNT          858 a         1465 c         1481 g          988 t
ORIGIN      
        1 agggcaggag ccaagcagcg cgggaagccg aacgcgcagg tccggtacag gtcccctcgc
       61 gcgtcaaact agatgatggc ggcgacccgg ctgtcattct cgatgagagg acgcgcaagc
      121 gaagcggggt cgaggtgatc atagcgctgc cgatccggta ccaggagcag gtccgcgcat
      181 acggaactgt gatggatctc gacaatctaa ccacgctcca caataggtat gtgacggcgg
      241 tggcgcagat ccaatccgcc caggccaagc tggtagcctc aaaagcggcg ttcgagcgcg
      301 cgcaggacct cttaaagagc aggtccatgg ctctcgctca gctgcaagcg gccgaggccg
      361 cgtttggcgt cgaccaggcc gcgctcgcga cggcgcaagc tcaggttaag accatcaggg
      421 cgacggcatt tgaggaatgg gggccggcga tcggaaaagc gcttgtcgag ggtgacgcgc
      481 tcgtcagccg gttgatcgag cgtgaagcct ttctaatcca gattacactt cggcctggga
      541 tctccatcga cccgccgccc acggccaccg ttcaactcag cgacagtgtg ccgcgtgttg
      601 aggtccgatt cctttcggtg gcgagccagg ctgatccgaa aatccaaggt ttcagtttct
      661 attacgtcgc cgatgccgag agccgcctat tgcccggcat gagtgtcctg gccgcgctgc
      721 cggtcggcgc cagtctagac ggcgttgttg ttccggcttc cgcaatcgtg tggtggggcg
      781 gtcgcacttg ggtctatctc cgcaccggtg ccgaaacctt caaacgccat ccgatcccaa
      841 ccgatctccc cgcaaccgat gccgggggcg tcgtcgtgcc agtcgcggtg ttcccgcgtt
      901 ccccccctga cttggtgact cgaggcgcgc aagcgctgct gtccgaggag ttgcgggcac
      961 agatccagat gggcgggaat gacctgaaat gaggtcatcc ggccgctctc cgtccagcgc
     1021 atcgggcttg caggccgctc tcatccggtt cgcaatccgt ttccgcgggg tggtcgtggc
     1081 gctggccgtg gttctgctcg gttacggtgt gctctccgtg acgcgggcga agtatgatgt
     1141 attcccggaa ttcgcaccgc cgcaggtaag tattcagacc gaaggacccg gcctgactcc
     1201 ggagcaaatc gaaactctgg tcacccaacc gatagagaac gcgatcaacg gcgtgcccgg
     1261 ggttcagacc ctgcgatctt catccatcca aggactctcc gtcgtcaccg tgatcttcga
     1321 tccggcaagc gacgtctacc gtgatcgcca actcgtggcc gagcgcttgg ccattgcagc
     1381 acggcaattc cccaccggga cccagccgcc ggctatgacg ccgctgacct cctcgaccag
     1441 caccgtcctc gttctcgggc tcacatccga caagcgctcc cttatggatt tgcgcaccat
     1501 tgccgattgg accctgcggc cgcggttgtt ggccgttccg ggcatcacaa aagtttcggt
     1561 cttcggcggc gaggttcgtt cgatccaggt ggcggttcat ccggacgaac tgatccgata
     1621 caacctcggc ttcaatgagg tgctgggggc ggctaggaag gccaccggaa tccgcggcgc
     1681 cgggttcatc gatacggcca atcagcgcat cgtgtttcag agcgagggcc agtcgcttaa
     1741 agcggacgac atcgcccgca ccgtgctggt caacgagggg gcgctgagtc tgcggctcgc
     1801 caacgtcgcc gacgtcgtgg acgcgccgga acccccgatc ggtggggcag ccatcatggg
     1861 ccagccgggc gtgatcctgg tggtgtccgc ccagtacggc gccaacacgc ttgaggtgac
     1921 acgcctggtc gagaacacgt tagcggattt acgacgggga ctcgaggagg atggtgtagc
     1981 gcttcatgcc gatctttttc gtcccgccga tttcgtagag acagcgacca ccaatgtccg
     2041 cgactcctta tttctcggcg gcattctggt tgtcatcgtc ctttttcttt ttctgttcga
     2101 cctgcgcacc gcggcgatct gctgcacggc gatcccgctt tcgctcctta ccgccgtcat
     2161 tgtcctgacg gcgttcggcg taacactcaa caccatgact ctgggcggac tcgcaatcgc
     2221 ggtcggcgag gtggtcgatg atgccgtgat cgacgttgag aacatcgtcc gccggctgcg
     2281 gcagaacagg caagatccga gcccccgttc gacggccagc gtcgtcctcg acgcttccct
     2341 ggaagttcga ggcgccgtcg tctatgcgag ctttgccgtc attctagtgt tcctgcccgt
     2401 aatcaccctg cccggcatcg ccggacgact gttcgctccg ctcgggtttg cctacgtcct
     2461 ggcaatcctc gcttctctcc tcgtcgcgct aacggtcacg ccggcactct gcatgatcct
     2521 gcttgccaaa aaggctcgcg aaagcaacga cgccgtcgtg cgtgaccggg acccgcccgt
     2581 cgtgaattgg acacgcagtc attacaagca cctgctgcgc ggcatcgtcg ctcacccgaa
     2641 gagcgccatt gccgtcgccg ccctcgtcac tctggctggc agcgctgtct tgccgttctt
     2701 cggagctgcg ttcctgcccg aactcaagga gggccacttc acactccaca tgtcggcggt
     2761 ggcgggcact tccatcgccg agtcacagcg gctcggaggg ctcgtgaccg gagcgctgac
     2821 cgagctaccg atggtgcgct cggtcgcaca gcgtatcgga cgggccgaga aggcggagga
     2881 tacctggggc actcactaca gcgagttcga agtcgccctc aaggccggtt tgtctgggga
     2941 tgagatagaa caggcgcaag ccgatttgcg caaggcgctg acccaatttc caggggtaaa
     3001 ctttgccctc aaaactttcc tgacggaacg aatcgaagag attctgtccg gctacacggc
     3061 ctcggtcgtt gtcaacatct ttggcaatga ccttgacctg ctcgacctcg tagcacgcga
     3121 cgttgcgcgg gtgatcggtg gcttttacgg cgccacggaa gtgcagttgc agtcgcggcc
     3181 cgggatgccc caattaacca tccggctgcg gaagcaagat cttgaacgct ggggttttga
     3241 tgtcatcgat gttctcgatg tcatccgtgc ggcgtaccag ggtgacgtcg tcggccaaac
     3301 ttatgaaggc aatcgcgttt tcaatgtcat tgtcacactc gatccgcaga atcgcaacga
     3361 catcatgaaa gttggcaatc tctcgatgcg gacaccgggc gggacctaca tccttctaag
     3421 tcagattgcc gacatctacc agtcagccgg gcgctatcag gtcgctcatc tcggagcgca
     3481 aaggctgcag acggtgaccg cgaacgtcgc ggggcgcgat gtggcggctt tcgtgcagga
     3541 cgcaaaagcg gtgctcgcag gtaatgtcga gttgccgagc ggtgtttacg tcgagttcgc
     3601 cggttcggcg gaggcgcagg cgcaatcccg acgcgatctt ttctttctct cagggattgc
     3661 gggggttgcg attgtgctgc tgttgtcaat cgtgacccgc aattggcgca acctcgtcct
     3721 tgttctcgtc aacatgccgt tcgccctggt tggcggcgtg ctggcggttt tcgcgaccgg
     3781 agggcagctg acgctgggag cgatggtggg tttcgtgact ctgttcggca ttactttgcg
     3841 caactcgatc atgatggtct cgcattacga gcatctggtc gaggtcgagg gtttgcattg
     3901 gggtgtggag gcggcgatcg agggcgcggg cgaccggctg acgccgatct taatgacctc
     3961 acttgtcacc gcccttgggc ttctgccgct cgcggttggc atggatgacc ccggccgtga
     4021 agtccagggc ccgatggctc ttgtcgtcct tggagggctg ttaacctcga tggccttgaa
     4081 tttgttaatt ctaccagcgc tcgccttgca ttttgggcga tttgagcggg cggatgatga
     4141 attcgaccag ccctcgcccg aggctacaag gaagtatcac gttgccagcg aataaattgg
     4201 tcacgcggcg ccgcagcagt ttggctaagc agagatcgaa gctcggacgc gtagacggct
     4261 cccgtcaagc tcggtcctcg accaactgct cgttagctcg cagagcagca tgggcgcgtt
     4321 cgcattcagc tattcgctgt gatccgatgc acgacctaaa acccaagcgc gtgcgtcatc
     4381 gtcagcgtcg accccgcttc gaagcccgat tgtgcgaagg catcggactg cttgcgattg
     4441 ctcttcgtgc aacgaaatcg acgtcttgat cagcctgaac tatctgcccg aggacgctaa
     4501 ggatgatcgt atacatgtga ggaggctgta acggcggcaa taggcccggc gcggtcagcc
     4561 agggcttcgg cgtcctcgcg gaagagacga agagagaact cacgttggga ttgctgcatc
     4621 ctgggcagtt taccgccgtg ggcgcgcgct cagaaccgcc acgagaaatt ggccttgacc
     4681 gaatgatcgc gcgcattgtt cgcgagctga ccgacatagg caactccgac cttcgtctgc
     4741 gggcccaact gcaggtcgcc acccgcttcg acgagtgcgg cgtcgcgcgc ga
//
LOCUS       DATLJT010000630         1748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1875644,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000630 DATLJT010000000
VERSION     DATLJT010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875644"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VM910_14580"
     CDS             <1..400
                     /locus_tag="VM910_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63791.1"
                     /translation="RRETLRVEAIEREYRSKLDDLRHNYALRVTVEWVQALNLYLPVQ
                     RFDVLIRRRKGERTVRLDWHPLAKTAEPPLCEAGLGRDRVRLVCDDKLHLTDPAGQAP
                     CRCCGKPFCRACFPACPRCGASAAQATFVG"
     gene            complement(468..845)
                     /locus_tag="VM910_14585"
     CDS             complement(468..845)
                     /locus_tag="VM910_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63792.1"
                     /translation="MNPKLIASRIARIALSTGRTTVDAPRPGHERRPGRRDADERQGK
                     RHSHEERQRRNQHQRYRDLERAGQGHQPAKDRQGDNEMHQRGSPTRRVLRAHSLALDS
                     AGRLYVADRSNSRIQIFESPGNI"
     gene            960..1748
                     /locus_tag="VM910_14590"
     CDS             960..1748
                     /locus_tag="VM910_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI63793.1"
                     /translation="MPTVPSGDIYFETHGAGTPVMLVPGLGGVGSYWNPNIPAFAQKY
                     QVVLHDHRGTGQSSRSRIHYTVDQMTDDLLAVMNHLEIEKAHLVGHSTGGAIGQTLAA
                     KHPERLISLVIYASWTKADPFFRRVFEARRALLTASGAAAYVRSTAIFLYPDWWINDN
                     IGLLEERENVAIPGFPLAEIAASRIDAIVDFNRTADLPKIRTPTLVICAKDDFLTPPY
                     FSRELARLIPGADFVELTRGGHCASETNTPEFNDAVLGFIARHS"
BASE COUNT          318 a          523 c          538 g          369 t
ORIGIN      
        1 acgtcgcgag acgctgcggg tcgaggcgat cgagcgcgaa tatcgctcca agctcgacga
       61 cctgcgccac aattacgctc tgcgcgtcac cgtcgaatgg gtgcaggcac tgaaccttta
      121 cctgccggtg caacgcttcg atgtactgat ccgccgccgc aagggcgagc gcacggtgcg
      181 gctcgactgg catccgctgg caaaaacggc cgagccgccg ctctgcgagg cgggcctcgg
      241 ccgcgatcgg gtccgcctcg tttgcgacga caagctgcac ctgaccgacc ccgctgggca
      301 agcgccctgc cggtgctgcg gcaaaccgtt ctgccgtgcc tgcttcccgg cctgcccgcg
      361 ttgcggggca agcgcggcgc aggcgacgtt cgttggctga ttgcgaacac gatggtttgt
      421 cccccgacga gacggttacg caacgggcac caccgagcca acagggctca aatgttgcca
      481 ggactctcga aaatctggat gcggctgttg gaacgatcgg caacataaag gcggccggcg
      541 gaatcgagcg cgaggctgtg ggcacgtaaa actcgccggg tgggactgcc gcgctggtgc
      601 atctcgttgt cgccctgccg atctttcgct ggttgatgcc cctgcccggc gcgctcaaga
      661 tcgcggtatc gttggtgttg attgcgccgt tggcgttctt catgggaatg ccgtttccct
      721 tggcgctcgt ccgcgtcgcg gcggcccggc cggcgctcgt gccctgggcg tggggcatca
      781 acggttgtgc gtcctgtgct gagcgcgatc ctggcgatcc tgctggcgat gagcttggga
      841 ttcaatgcgg tggttctgat cgcgattggc ctctatgtcg tcgccgcggc gacgcttcgc
      901 taatctccat atgaggtcat aaagaatcct gctcacacac agagaaagag agagaagtca
      961 tgcccacagt cccttctggc gatatctatt tcgagacgca cggggcagga actcccgtta
     1021 tgttagtccc cgggctcggt ggcgtcggca gctattggaa tccaaacatc cccgcattcg
     1081 cgcaaaagta ccaagtggtc cttcacgatc atcgaggaac gggccagagc agccgctcgc
     1141 gtatccacta taccgtcgat cagatgacag acgacctgct ggcggtgatg aaccatctcg
     1201 agatcgagaa agcgcatctg gtcggtcatt caaccggcgg tgcaatcgga cagaccctcg
     1261 ccgccaaaca tccagagcgt ttgatcagcc ttgttatcta tgcgagctgg acgaaggccg
     1321 atccattctt ccggcgcgtg ttcgaggcgc gacgggctct gctgactgcg tcgggcgctg
     1381 cggcttacgt gcgttcgact gcgatttttc tctatccaga ctggtggatc aatgacaata
     1441 tcggcttgct cgaagaacga gagaacgttg ccattccggg atttccgctt gcggagattg
     1501 ctgcaagtcg gatcgacgcc attgtggatt ttaatcggac agcggacctg ccgaagatca
     1561 ggactcctac tctggttatt tgcgcgaagg acgacttcct gaccccaccc tattttagtc
     1621 gggagcttgc ccgtctgatc cctggtgccg atttcgtaga gttgacacgc ggtggacatt
     1681 gcgcgtcgga aacgaacacg ccagagttca acgacgccgt tcttggtttc attgcgcgtc
     1741 attcgtag
//
LOCUS       DATLJT010000631         3198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3713685,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000631 DATLJT010000000
VERSION     DATLJT010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3713685"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VM910_14595"
     CDS             <1..814
                     /locus_tag="VM910_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63794.1"
                     /translation="IGSSVTEVASSDNSDISEKEFQDSQVVDDGITPQPKDFSEVSPE
                     SVGARVKLDAALDFLKSLKGQDVEVGLGSLAVTCDALRAGQQTINMSVDPDRAAALIG
                     KMKAMPGVTLAGWTTGVVEMDRAIRFSASDWRDGDKLNRDKLVATISRVLAKTLSAQF
                     VSSSWSTTTGKLKLTFKRPSPVYPALELTDTIEVTGLVTPDKPGGSDKLMLWLGNPAT
                     STADEGAGAKLILSDESSGEEEGDQRDDSGSIEALTKELKGQRWDADKTQWK"
     gene            complement(879..1292)
                     /locus_tag="VM910_14600"
     CDS             complement(879..1292)
                     /locus_tag="VM910_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HVI63795.1"
                     /translation="MVCFKSVVRVADALILLTCFSSGRSASAESGFPFGMEMTLDATR
                     QPGSKRVPDLDIGENGEVVLELWCKGGKGQFSVAGNTVIFVAGPMEDRACPPARAQLD
                     DELVAALSETTTWTREGDVVSFIGSKTLRFRLNTN"
     gene            complement(1559..3184)
                     /locus_tag="VM910_14605"
     CDS             complement(1559..3184)
                     /locus_tag="VM910_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVI63796.1"
                     /translation="MSTIAAAVPVVTLLVLIASGRVKAHVAAIIAVIVTNLIAIFVFT
                     MPAGMSARASLLGIVTGFFPIGWIVLNVIFLYQITVTTGRFELLKRAVGGVTEDRRLQ
                     LLLVAFSFGAFFEGASGFGTPVAITGSILIGLGFSPLAASGLSLIANTAPVAYGALGA
                     PIQGLSSVTRLDPYILGAMVGRQLPVFSLIVPFWVVWAFAGWRGMKEVWPAILVTGVS
                     FAIPQFLISNYINPWIVDIGASLISMGCLILFLRVWHPRQLWLSPALRGRDESAATLA
                     ATKPLDKTSLTQAELWSALLPWVIVCIVMLLWGSGWFKTWANSIFVWNYAVPDLHQMI
                     NKMPPVVAKPTPEGAVFAFTYLSFTGTGMLIAAIISGLLMGLSPVKLVSEYGRTIKIC
                     AISLITISAMLAIGTLTRLSGIDATLGLAFAATGVLYPFFGTLLGWLGVALTGSDTAS
                     NILFGNLQKITSEQLGLSSILMTAANSSGGVMGKMIDAQSIVVASTATNWYGHEGTIL
                     RFVFRHSIVLACLVGVLVTLQAYVYPFTAMVIR"
BASE COUNT          704 a          979 c         1012 g          503 t
ORIGIN      
        1 catcggctcg agcgttacgg aagtggcctc cagcgacaat tccgacatca gcgaaaagga
       61 attccaggac agccaggtgg tcgacgacgg catcacgccg cagccgaagg atttcagcga
      121 agtctcgccg gaatcggtcg gtgcgcgcgt caagctcgac gcggcgctgg atttcctcaa
      181 gagtctgaag ggccaggacg tcgaggtcgg gctcggcagc cttgcggtta cctgcgatgc
      241 gctgcgcgcg ggccagcaga ccatcaacat gtcggtcgat cccgaccgcg ccgccgcgct
      301 gatcggcaaa atgaaggcga tgccgggcgt caccctggcg ggctggacca caggcgtcgt
      361 cgaaatggat cgcgcgatcc gcttttccgc ttccgactgg cgcgacgggg acaagctcaa
      421 cagggataag ctggttgcga cgatttcccg cgtgcttgcc aaaacgcttt cggcgcaatt
      481 cgtaagctcg agctggagca cgaccaccgg aaagctgaag ctgaccttca agcggccgag
      541 cccggtctat cccgcgctcg agctgaccga cacgatcgag gtcaccggcc tggtgacgcc
      601 cgacaagccc ggaggatcgg acaaattgat gctctggctc ggcaaccccg cgacctcgac
      661 cgccgacgag ggcgcgggcg ccaagctcat tctgtcggat gaatcctcgg gcgaagagga
      721 aggcgaccag agggacgatt ccggctcgat cgaagcgttg acgaaggaac tgaaaggcca
      781 gcgctgggac gccgacaaaa cgcaatggaa ataaccgagc ggcgctgact cgcgcgtcag
      841 cggaggttca tctcttccac ggaggtgaac cggatctcct aattcgtatt gagccggaag
      901 cgcagcgttt tgctgccgat gaaggacacg acatcgccct cgcgtgtcca ggtggttgtt
      961 tcggagagcg ccgccaccaa ttcgtcgtcg agctgcgcgc gcgccggagg gcaagcacgg
     1021 tcctccatcg gacccgcgac aaagatcacc gtgttgcccg caaccgagaa ttggcctttg
     1081 ccgcccttgc accacaattc gagaacgacc tcgccattct cgccgatgtc gaggtcaggt
     1141 acccgcttcg atccgggctg gcgggttgca tccaatgtca tctccatgcc gaacggaaag
     1201 ccgctctccg cgctggcgga acggccgctc gaaaagcatg tgagcaggat taatgcgtcg
     1261 gccacgcgaa ctacggattt gaagcaaacc atgccacctc tcaaattgta acgagccacg
     1321 cgcgtccttc tattggacgc gtgcgtcgtt gaacaggttc gtgtcggaaa aaattagagc
     1381 atgatccgga aaagcgggca ccggttttcc gtcgcgacaa atgtgggaac gcgtttgcgc
     1441 ggagattatg ctcaaacaag aaccagagcg cgatagcgat tcgagctatc gcgctctggt
     1501 gcggcaaaaa tccccgcgag gatttgcgcc tcgcggggat ttcaattcga cgcggtcgtt
     1561 acctgatgac catcgccgtg aacggataga cataggcctg cagcgtcacc aggacgccga
     1621 ccagacaggc cagcacgatc gaatggcgga acacgaagcg caggatagtg ccttcgtggc
     1681 cgtaccagtt ggtcgcggtg gaggccacca cgatcgactg cgcatcgatc atcttgccca
     1741 tcacgccgcc ggacgagttg gccgcggtca tcaggatcga tgacaggccg agctgttcgg
     1801 aggtgatctt ctgcagattg ccgaacagga tattggaggc ggtgtcggaa cccgtcagcg
     1861 ccacgcccaa ccagccgagc agcgtgccga agaagggata gagcacaccg gttgcagcga
     1921 aggccaggcc cagcgtggcg tcgatgcccg acagccgcgt cagcgtgccg atcgcgagca
     1981 tggccgagat cgtgatcagc gagatcgcgc agatcttgat ggtgcggcca tattcggaga
     2041 cgagcttgac gggcgacagg cccatcagga ggccggagat gatagccgcg atcagcatgc
     2101 ccgtacccgt gaaggacagg taggtgaacg cgaacaccgc gccttccggc gtcggcttcg
     2161 ccaccaccgg cggcatcttg ttgatcatct ggtgcagatc gggaaccgca tagttccaga
     2221 cgaagatcga gttcgcccag gtcttgaacc acccgctgcc ccagagcagc atcacgatgc
     2281 agacgatgac ccatggcagc aaagcgctcc acagttccgc ttgcgtcagc gacgtcttgt
     2341 cgagcggctt tgtcgccgcc agggtagcgg ccgactcatc gcgaccgcgc agcgccggcg
     2401 acagccagag ctgccttggg tgccacactc gcaagaacag gatcaggcac cccatcgaga
     2461 tcagggacgc accgatatcg acgatccagg gattgatgta gttcgagatc aggaattgcg
     2521 ggatcgcgaa ggagacgccg gtgacgagaa tggcgggcca gacctccttc atgcctcgcc
     2581 agccggcaaa cgcccatacc acccagaacg gcacgatcag cgagaacacc ggcagttgcc
     2641 ggccgaccat cgcgccgagg atatagggat cgaggcgcgt gaccgaagag aggccctgga
     2701 tcggagcgcc gagcgcgcca taggcgaccg gcgcggtgtt ggcgatcagc gacaggccgg
     2761 aagccgcaag cggcgaaaag ccgagaccga tcaggatcga gccggtgatt gcaacggggg
     2821 tgccgaaacc ggacgcgccc tcgaagaacg cgccgaacga aaaagccacc agcaagagct
     2881 gcaaccttcg gtcttcggtg acgccgccga ccgcgcgttt caacagctcg aatcgtccgg
     2941 tcgtgaccgt gatctggtac aggaagatga cgttgagcac gatccagccg atcgggaaga
     3001 agccggtcac gatccccagc agcgaggccc gagcagacat gcctgccggc atagtgaaga
     3061 cgaagatcgc gatcaggttg gtgacgatca ccgcgatgat cgccgccaca tgcgccttca
     3121 cccggccgct tgcgatcagg accagaagcg ttaccaccgg cacggcagca gcgatcgtcg
     3181 acaatgccgc gttgccca
//
LOCUS       DATLJT010000632         1531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2105445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000632 DATLJT010000000
VERSION     DATLJT010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VM910_14610"
     CDS             <1..483
                     /locus_tag="VM910_14610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63797.1"
                     /translation="NSPLSADPFIHKNLSYDPIKDFVPITRVGSFTLMLVVNAKLPIH
                     SVKQLVEYAKVHPGELSFASGNTAGIVGGHTLAKWGEMSMLHVPSRRGALALLRVRDR
                     GFPDALARLEIVGEDPAVLGAAEKHAVQIRRAAIDALGRGRSVILMRAPVLAACRRID
                     "
     gene            complement(1109..>1531)
                     /locus_tag="VM910_14615"
     CDS             complement(1109..>1531)
                     /locus_tag="VM910_14615"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HVI63798.1"
                     /translation="PRAIVGALIGTIVFTLMMKFLAPMMIGHPMDIAAVLGTFTGLGT
                     SAGMIMHFLLGTIGFAVAFLIVGPYLPGPIWLRGVIFMAGVWFLAGLIAMPILGVGLF
                     FGGAREAMAALMGHVAFGAILGAIARLPSRHVQAWSLR"
BASE COUNT          340 a          459 c          454 g          278 t
ORIGIN      
        1 aactcgccgc tatctgccga cccgttcata cacaagaact tgagctatga tccgatcaag
       61 gactttgtgc cgatcacgcg ggtgggaagt ttcacgctga tgctcgtggt caatgcgaaa
      121 ctacccatcc attcggtcaa gcagctggtc gagtatgcca aggtccatcc tggcgagctt
      181 tcgttcgcca gcggcaacac ggccggcatc gtcggcggcc acacgctggc gaaatggggc
      241 gaaatgagca tgttgcatgt gccgagccgc cggggcgctc tcgcccttct tcgggtccgc
      301 gatcgcggtt tcccagatgc gcttgcccgt cttgaaatcg taggcgagga cccggccgtc
      361 ttgggtgccg cggaaaagca tgccgtccag atacgccgcg ccgcgattga cgccctgggg
      421 cgtggccgga gcgtaatcct catgcgtgcg ccagttctcg cggcatgtcg aaggatcgat
      481 tgaaaagatg tcgaacgcgg tcacgaagat cagcgccccg ttcacctcca acaacccaga
      541 agtgaagccg gtatattgcc tggtatcata agtgcagaga actttgagct tgtcagcgtt
      601 tgttctgttg atctgactta actgcgagaa gcggttcgag gtgagcgtct tgttgtagct
      661 aggccaatct ccctcagttc cgccaggagc agcctgcggc gatgccgaag ccgtcgaggc
      721 tgacggctgt cccaccgctc cggtctgggc gacctccgtt tcaagggtcc cggcgggagc
      781 tgaccaatat cgcacataat ttatcgccat tgacgcgatc agaaccgttt ggtcccaata
      841 agcataggac agactggcta tggcgatgcc ggcgacaacg attgcgccga agagagcgcg
      901 aagccaaaac caagatctca tcggttaact ccctgaaccg acatcgccgg cgcggccggt
      961 ctcggcgaga atgatccgag ctccgtggcg gaaggtgaca taggcccaca gccaatcgag
     1021 cgtcacgacg atgcgcgacc ggaatcgcgc cggacgggtg gaggtttgcg acgatctatc
     1081 ggttccacga cacgttgccg aacgacattc atcgcagcga ccatgcttgg acgtgtcggg
     1141 aaggcaagcg cgcgatcgcc cccaggatgg cgccgaaagc gacatgcccc ataagcgcag
     1201 ccatggcttc cctggcacct ccgaaaaaca agccgacgcc gaggattggc atggcgatga
     1261 gaccggccaa gaaccagact ccggccatga atatcacgcc acgcagccat atcggacccg
     1321 gcaagtacgg gccaacaatc agaaacgcga cggcaaagcc aatcgtgccg agcaggaagt
     1381 gcatgatcat cccggccgaa gtgcctaatc cagtgaatgt tccgagcacg gctgcgatat
     1441 ccatcggatg tccgatcatc atcggcgcca ggaatttcat catcagcgtg aagacgatgg
     1501 tcccgatcaa agccccgacg atggcgcgcg g
//
LOCUS       DATLJT010000633         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_268354,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000633 DATLJT010000000
VERSION     DATLJT010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268354"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VM910_14620"
     CDS             complement(<1..743)
                     /locus_tag="VM910_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63799.1"
                     /translation="MSDRRPSPFKTLFDTEAATLIQAAPDVYQIRFKNRAANAYLVVG
                     TKRTILVDVGLSTNYPHLLACMNHVGITPDKIDMLVLSHEHLDHIGAAYHFNNNRTFV
                     AAHRLAANKIMLRDDFSMLRKMFNEPNVPIGIDIWLEEGNMIDLGNFRLDVMHTPGHT
                     SACMTLFDPDKGLLFAADTLMPGGVMGGVFGSGSIADYIQSLERLKGLNSKILLSGHG
                     RLSDAPQDDVRIAIQRSHTLLSDTAQLFDA"
     gene            complement(740..1114)
                     /locus_tag="VM910_14625"
     CDS             complement(740..1114)
                     /locus_tag="VM910_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI63800.1"
                     /translation="MAIEKCINEYGVDDVIFEEGSTGRELYVVLDGKVEIAKVHGASK
                     TVIVTLGKGEFFGEMAVIDGSARSATAIAASPNTRVMRINHARFVYLVSQQPAFALMI
                     MDALSKRLRVSNERTFKAATNS"
BASE COUNT          314 a          438 c          493 g          297 t
ORIGIN      
        1 gcgtcgaaca attgcgcggt atccgacaac agcgtgtggg agcgctggat cgcgatccgt
       61 acatcgtcct gcggcgcgtc ggacagccgg ccatgtccgg acagcaggat cttcgaattg
      121 aggcccttca gccgttccag cgactggatg tagtcggcga tcgagcccga gccgaacacc
      181 ccgcccatca cgccgcccgg catcaaggtg tcggcagcga acaacaggcc cttgtccggg
      241 tcgaacagcg tcatgcaggc cgaagtgtgg cccggcgtgt gcatcacgtc gaggcggaaa
      301 ttgccgaggt cgatcatgtt gccttcttcg agccagatgt cgatgccgat cggcacgttg
      361 ggctcgttga acatcttgcg cagcatcgag aaatcgtcgc gcagcatgat cttgttggcg
      421 gcaagccggt gcgcggcgac aaaggtgcgg ttgttgttga agtgataggc cgcgccgatg
      481 tgatcgagat gttcgtggga aagcacaagc atgtcgatct tgtcgggcgt gatgccgacg
      541 tggttcatgc aggccagaag atgcggatag ttggtggaca gtccgacgtc gaccaggatg
      601 gttcgcttgg tgccgacaac gaggtaagcg ttcgccgcgc ggttcttgaa gcggatctgg
      661 tagacgtccg gcgccgcctg gatcaaggtc gcggcttcag tatcgaagag agtcttgaac
      721 gggcttgggc gccggtcgct catgaattgg tcgccgcttt gaatgtacgc tcgttggaca
      781 cgcgcagccg cttgctcagc gcatccatga tcatcagcgc aaaggccggt tgctggctga
      841 ccagatagac gaagcgcgcg tgattgatgc gcatcacgcg ggtgttgggg ctcgccgcaa
      901 tcgcggtcgc cgagcgcgcg gagccgtcga tcacggccat ctcgccgaag aactcgccct
      961 tgccgagggt gacgattacg gtcttgctgg caccgtggac cttggcgatc tcgaccttgc
     1021 cgtcgagcac cacgtagagt tcgcgcccgg tcgagccttc ctcgaaaatg acgtcgtcga
     1081 cgccgtattc gttgatgcat ttttcgatgg ccatggcgtt ctcgtttcga cccgtcgcaa
     1141 ggccatgtta atagggaaat cccgatgcgc aaacgacact tagggggaac tgacgggctc
     1201 ttaccgcagg gcagcatacg gccgaggggt ggacgcgtgc cgcacctcga ccgccatgtc
     1261 ggccaaaaac ggccccgaaa gagtccctaa aatttgtgta agaaaacgcg gaaaatgcgg
     1321 tccgtaacga atttgcaagg attcgaaagt tagcaaatgg tcaatcaaac ctggaggatt
     1381 tgcccgtgag cgaacaacag gccgagcaga ccagcggcga acccggttcc gtcggtagcg
     1441 ggtcgaccgt tcccacttcc cccgatccac tgattgatac ggtgatggca tcgatgcgag
     1501 aggccagcac caccgaaacg gcggtgatca cctccgacca cg
//
LOCUS       DATLJT010000634        13028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_726549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000634 DATLJT010000000
VERSION     DATLJT010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VM910_14630"
     CDS             complement(<1..295)
                     /locus_tag="VM910_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI63801.1"
                     /translation="MKLTGKCFCGAVEIEASGSPEAMGYCHCRSCRSWSGGPVNAFSL
                     WKPEAVRISSGADNVATFQKTSLSQRQYCKKCGGHLMTNHPPLGLIDVFAATLP"
     gene            533..1498
                     /locus_tag="VM910_14635"
     CDS             533..1498
                     /locus_tag="VM910_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI63802.1"
                     /translation="MTDLSPRILEFSTSGVPERDRIAIWREHYGSVMLRVDIEPAADR
                     IFEASNRCLSLPGLQLMESASSPAKVSRRGRYLADGNDDVVLAINRAGSVVVASGGRE
                     QSLREGHAIVLSGGEPASFHRTSLGQSFTLRVPRAIFESTVVSVDDALMRPLPEHGAL
                     KLMEDYARWLFNASGTIDQALLNLSVRHIHDLLALAIGPTAEFADTARTRGLRAARLK
                     LAKSFIIAHSDRREISVAMVAARLNVTPRYVQRLFEADGTTFSEFLTRQRLARAHRLL
                     CEPSVSQAAISNIAYDVGFGDLSYFNRRFRREYGLTPREVRGDKT"
     gene            1621..2154
                     /locus_tag="VM910_14640"
     CDS             1621..2154
                     /locus_tag="VM910_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63803.1"
                     /translation="MNGIFLAAAVAVAATTSVASGLLVTGARGPSSLRRSARLGLSRS
                     CRRLKRLVDTGVAAMLARRERQAATSLALREMAGGVCGDISSGTKRRSPDLVDRRPSS
                     LHTPDRRAVSLKRIVFRIIDAVLIGFTILLMIVVSGLPIEWLLDFDDVSYIEAGVALI
                     SIVSLAVIYAVVADTPS"
     gene            complement(2169..2561)
                     /locus_tag="VM910_14645"
     CDS             complement(2169..2561)
                     /locus_tag="VM910_14645"
                     /inference="COORDINATES: protein motif:HMM:NF027832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HVI63804.1"
                     /translation="MNAVTGTQSPSNTTKTPVIEGLYSLEIEMSGTKRGRATGVAIIQ
                     NGRIMGGDSYFYYTGSYTFSRGKWRGEMIVHQHTDAPGLNLAFGGREVSCGFTGTFSG
                     GSAEIDGTALVGTSSVPLHARLTLQVPL"
     gene            complement(2655..3497)
                     /locus_tag="VM910_14650"
     CDS             complement(2655..3497)
                     /locus_tag="VM910_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI63805.1"
                     /translation="MIKDILVNVPTERPQRPVVDASVSLAASFGAHLDALAIGYISTA
                     AAYVPDGGAAAAVAAVFEVERERATERAATALSIFETEARNAGIAYASHPSADFAANA
                     AVSIGAAARLYDLAIVLQPEAERETFDNTIPREILFQAGGPVLFIPHIFRGAFKAKRI
                     GICWDGSRLAARALRDARPFLAQADTLVAISINGADSTPAEASADKLAKHLALAGLPV
                     RIIELPAARAEIQPSILSLAADENLDMLVMGAYGHSRLQEGLLGGVTRAMLRTMTVPT
                     LMSH"
     gene            complement(3686..5407)
                     /gene="sulP"
                     /locus_tag="VM910_14655"
     CDS             complement(3686..5407)
                     /gene="sulP"
                     /locus_tag="VM910_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HVI63806.1"
                     /translation="MIRWLPGVDTFRRYEATWLPHDIFAGLVLATMLVPVGIAYAAAS
                     GLPGIHGLYATIVPLLAYAVFGPSRIIVLGPDSALAAVILGVVMPLAGGDPGRAATLA
                     AMMALVSGTVLILAGLARLGFVTELLSKPIRYGYINGIALTVLISQLPKLLGFSIESE
                     GPLRDLWAIARAIHDGKTNWVAFAVGLGTLIVILLLRNSKRLPGILIAVVGATVIVGL
                     LDLSARYGVKVLGPLPQGLPAFAVPWIGYTDLVPVLIGGCAIALVSFADTSVLSRTYA
                     ARLGDDVDPNQEMVGLGAANLATGFFQGFPISSSSSRTPVAETAGARSQLTSVVGALA
                     IAFLLVLAPNLLRHLPSAALAAVVIASAIGLFEIADLKRIYRIQRWEFWLTILCFVGV
                     AVFGAIPGIGLAIAFAIAEFLWDGWRPHSAVLGRAYGVKGYHDITRYPDARRIPGLVL
                     FRWDAPLFFANAEFFRERVLDAVAKSPTPVRWLVVAAEPVTSVDVTACDTVAELDVAL
                     HERGIELCFAELKDPVKDKLKRFGLYAQLGESYFFPTIGSAVSRYLEVTNVEWNDWED
                     DEAKLRA"
     gene            6000..6227
                     /locus_tag="VM910_14660"
     CDS             6000..6227
                     /locus_tag="VM910_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63807.1"
                     /translation="MTRLIEYNARAALCRRLAKLEPVSRDIWLAEAERWTRLAQASMG
                     AQDNEDGAEAPEGASQSLGDAVKEDAVKQKD"
     gene            complement(6254..6769)
                     /locus_tag="VM910_14665"
     CDS             complement(6254..6769)
                     /locus_tag="VM910_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI63808.1"
                     /translation="MVKNYSVVEHLRDGSTIEIRALQPDDREDMLAAVGRTSTQSLQR
                     RFFVPKRGFTDKEVSFFINIDFSNHVALVAHVDEDGDSVIAGGGRYIIVRPGQAELAF
                     VVVDAYQGKGIATILMRHLLVIAREAGLKELTAEVLPENAAMLKVFSKFGFRAGSGSD
                     PQVRHLTLQLA"
     gene            6964..8073
                     /locus_tag="VM910_14670"
     CDS             6964..8073
                     /locus_tag="VM910_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVI63809.1"
                     /translation="MALRGFRQNRSSEEVIQIAIRLGLLAALIVWCFIILRPFIAILA
                     WSAVLAVALYPLYAWLSERLGGRPKLAACIITVIATAIVIGPVTWLGFGLVEGLRTIY
                     EQADSEMLQVPSPPASIKEWPLVGERIYALWNQASTNLGAFLGQLAPYVKPIIRSMLG
                     MAGSVSLGALKFLISVILAGFLLAPGPRFVTGIRTMMARVLPQRSEELVTLGGATIRT
                     VSQGVIGIAVVQSFLFGVGLKFAGFAHAGLIAFLVLVLSIVQLGTSIVLIPCVIWIWM
                     TKDTATALFISIYLVLVGLADNVLKPLLMGRGLSTPMPVMFLGLLGGTLAHGIVGLFI
                     GPIILAVGWELMMAWVRDERTEDVPAPAERVPAEI"
     gene            8205..10427
                     /locus_tag="VM910_14675"
     CDS             8205..10427
                     /locus_tag="VM910_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HVI63810.1"
                     /translation="MSLDKQTVSDGVPPSFFTSALEYLVDAGQRSVLFLDVMRQRGVQ
                     FREHMAETVPNVLDYGVELVVDGRNLERPVNYGLVRIVPPKGVEIDLKRRPFVVVDPR
                     AGHGPGIGGFKADSEIGVAMKAGHPCYFVGFMPEPMPGQTIQDIARAEGVFLEKVIAL
                     HPQADGKPCVIGNCQAGWAVMILASLRPELFGPIIIAGAPLSYWAGVRGKNPMRYTGG
                     LLGGSWLTALTSDLGAGKFDGAWLVQNFENMNPSNTLWTKQYNVFSKIDTEAPRYLEF
                     ERWWGGHVNLNAEEIQFIVDELFIGNHLATGRIKTSDGQVVDLRNIHSPVVVFCSKGD
                     NITPPQQALGWILDLYHDVDEIRAYGQTIVYTVHQTVGHLGIFVSGGVAKKEHSEFSS
                     NIDLIDVLPPGLYEATFEERSADTTNPDLLSGKWIMRCERRTLDDIRAMGGNSLEDER
                     RFATAAKVSEINLAAYRTFVQPWIKAMANPQMAELARKWHPLRVQYEALAGQNPLTKA
                     IETTADKVRENRKPAATDNPFLALQETVSKSIVGALDQWRDAQEALSERLFLSVYGSP
                     ALQAAVGIDPEADPSPMPEMSQDYHKRLEARIAELKSRIASGGLRECVVRGLLYVGAS
                     RGMVDERSLEALRRARTNDSGSRLTLAEFKAMVREQFFMLLLDQEASLAAIPKLLPKD
                     AKARRAGLAVIREVLSASAEISGETANRLRRVTELFGGTEDSASQASANPLDPQAKAS
                     "
     gene            10442..11407
                     /locus_tag="VM910_14680"
     CDS             10442..11407
                     /locus_tag="VM910_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HVI63811.1"
                     /translation="MSAQPAGETRPHSKYDRLIAAAKAVPTVTTIVVHPCDETSLRGA
                     IEAAEAGIIKPILVGPAAKIAAAAAKHNINIAGYEIVDAEHSEAAAAKGVELIHQAKG
                     EVLMKGSLHTDELMRSVTAKATGLRTDRRISHVFVMDVPTYTDTVFITDAAINIFPDL
                     DGKRDIIQNAIDLFNQAGFGTVPRVAVLSAVETVTSKIPSTIEAAALCKMADRGQITG
                     GLLDGPLAFDNAIDVEAARIKGIKSEVAGHAQILVVPDLEAGNMLAKNLAYFAKADGA
                     GVVLGARVPIVLTSRADSPRARMASCAVAALQADARRRRAVNAAA"
     gene            11413..12624
                     /locus_tag="VM910_14685"
     CDS             11413..12624
                     /locus_tag="VM910_14685"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HVI63812.1"
                     /translation="MDTILVVNAGSSSVKFQVFAVDGDGRLQRQLKGQMDGIGSRPRL
                     RATGAKGDTLADRAYPIEAVADVAAGLMVAGAWLRDELRITPMAVGHRVVHGGPDYDR
                     PVLVDHGLVARLERYVALAPLHQPYNLAPIKSLLANLPALPQVACFDTAFHRGHDGVA
                     DQYAIPRQLYDEGVRRYGFHGLSYEYIARTLPKVAPDIAAGRVIVAHLGSGASMCALK
                     SGRSVESTMGFTALDGLPMGTRPGQIDPGVVLYLLSEKRMEPGKVQDFLYRDCGLKGL
                     SGVSNDMRELLESRDPRAAFAIDYFTYRVGLCAGMLAAALQGLDAFVFTAGIGENSDA
                     IRSRVAERLAWLGVSLDAAENAKRSLKISQSGSRIPVYVVPTDEERMIAEHTLSVIWG
                     ANSKAKREIAS"
     gene            12621..>13028
                     /locus_tag="VM910_14690"
     CDS             12621..>13028
                     /locus_tag="VM910_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI63813.1"
                     /translation="MSIPEYPETKVALKGKKGLIVGIANDRSIAWGCAKAFRALGADL
                     AVTYLNDRAKKHVEPLAQQLEAQIFVPLDVSVEGQTEQVFERIAKEWGQLDFLLHSIA
                     FSPKEALHGRVIDVGRDGFLKTMDISCWSFMRMA"
BASE COUNT         2484 a         3968 c         4136 g         2440 t
ORIGIN      
        1 cgggcagcgt tgcggcgaaa acatcgatca ggccgagcgg cggatgattg gtcatcaggt
       61 gcccgccgca ctttttgcaa tattgccgct ggctcaggct cgttttctgg aacgtcgcga
      121 cgttgtcggc gcccgatgag atgcgcacgg cttcgggctt ccagaggctg aaggcattga
      181 cgggcccgcc cgaccacgaa cggcaggaac ggcaatggca atagcccatc gcttccggtg
      241 aacccgacgc ttcgatttca accgctccgc aaaagcattt gccggtcaat ttcatgtgag
      301 atccctcact gctgcaaaca accgagaata tcacggatca ggccgcgatc ttggacttgc
      361 gcgaccaatc ccgggcgcga cacgacagcg cgccgtcgag cgtgcggtcc gtcacattgt
      421 cctctcttcg ggggatgccg tggagtgcgc gaatccgcta acaggcggcg gggaggggag
      481 cgcgtagaag gtggggcgca gcggatgacc gtgtttttag gatgcaaggc cgatgaccga
      541 tttgagccct cgtattctgg aattctccac cagcggcgtt cccgagcgcg accggatcgc
      601 gatctggcgc gagcattatg gaagcgtgat gcttcgggtc gatatcgaac ccgcagccga
      661 caggattttc gaggcgagca atcgatgcct gtcgctcccc ggcctgcagc tcatggagtc
      721 ggcgtcatcg ccggccaagg tttcgcgccg cggccgctat cttgctgacg gcaacgacga
      781 tgtcgtgctt gcgatcaacc gcgccggctc ggtggtcgtc gcctccggcg gccgcgagca
      841 aagtctgcgc gagggtcacg cgattgtgct gagcggcggg gagccggcat cgtttcaccg
      901 cacgagcctt gggcaatcgt tcacgctgcg ggtgccgcgc gcgattttcg aatcgaccgt
      961 ggtcagcgtc gacgacgcgc tgatgcggcc gcttcccgag cacggcgcgc tgaagttgat
     1021 ggaggattac gcgcgctggc tgttcaacgc ctccggtacg atcgatcagg cgctgctcaa
     1081 tctttcggtt cgccatattc acgatctgct tgcgctggcg atcggcccga cggccgagtt
     1141 cgccgacacg gcgcgaacgc gcggcttgcg cgcggcacgt ttgaagctcg cgaaatcgtt
     1201 catcatcgca cacagcgatc gccgcgagat atcggttgcg atggttgccg cacgactcaa
     1261 tgtgacgccg cgctatgtgc agcgtctgtt tgaggcggac ggaacgacat tttcggaatt
     1321 cctgacccgg cagcgccttg cacgcgcgca tcgcctgttg tgcgagccgt ccgtgagcca
     1381 ggccgcgatc agcaacatcg cctacgatgt cggcttcggc gacctgtcct acttcaaccg
     1441 ccgcttccgc cgcgaatatg ggctgacgcc gcgggaggtg cggggcgaca agacgtgatg
     1501 ccgttgttct ctgtggtccg agaggatgtc gcgcggaacc aagacggatt ctggcccgca
     1561 cgttagtttc ggcaccattt cgagccgtct gacgattact gcgatgcgaa tggaattgcg
     1621 atgaacggaa ttttcctggc ggcagccgtc gctgtcgccg caaccacctc ggttgcttcc
     1681 ggcctgcttg tgacgggagc gcgcggaccc tccagcctcc gtcggtctgc acggctcggc
     1741 ctttcacgat cctgccgacg gctgaagcgg ctcgtcgaca cgggggtcgc agccatgctc
     1801 gcccgtcgcg agcggcaggc cgcaacgtcg ttggctctac gcgagatggc cggcggcgta
     1861 tgtggggata tctcgtcagg tacgaagcgc cggtcgccgg acctcgtgga ccggagaccg
     1921 tcaagcctgc acacgccgga tcgcagagcc gtgtcgctga agaggatcgt cttcaggatc
     1981 attgacgccg tcctgatcgg cttcacgatc ctgctgatga tcgtcgtctc gggattgccg
     2041 atcgaatggc tgctcgattt cgacgacgtg tcctatatcg aggcaggcgt tgcactgatc
     2101 tcgatcgtct cgctcgcagt gatttacgcg gtcgtcgcgg atacgccgtc ctgatccggg
     2161 gacgggtttt acagcggcac ctgcagcgtc aatcgcgcat gaagcggaac gctcgacgtg
     2221 ccgaccagcg ccgtaccgtc gatctctgcc gagccgccgg aaaaggttcc ggtgaagccg
     2281 caactgacct cgcggccgcc gaaggcaagg ttcagtcccg gggcgtccgt gtgctggtgc
     2341 acgatcattt ctccccgcca cttgcccctg ctgaaggtgt aggaaccggt gtagtagaag
     2401 taactgtcgc cacccatgat gcggccgttc tgaatgatgg ccacgccggt cgcgcggccg
     2461 cgcttcgtgc cgctcatctc gatttcgaga ctatagagcc cctcgatcac cggcgtcttg
     2521 gtggtgtttg acggactttg ggtccctgtc acagcgttca ttgccctctc cttgcggagc
     2581 ggccattaca gcgagtcaat cgcctttttg caatgcgaca agggcgctcg cttacgagca
     2641 ggaccgatga tgcctcagtg agacatcagc gtcggcaccg tcatggtgcg cagcatcgcg
     2701 cgggtcacgc cgcccaggag cccttcctgc agccgcgaat gtccgtaggc tcccatcacc
     2761 agcatgtcga ggttttcgtc ggctgccagc gacaggatgg aaggttggat ctcggcgcgc
     2821 gccgccggaa gttcgatgat cctgaccggc aagccggcaa gcgccagatg cttggcgagc
     2881 ttgtccgctg aagcttccgc gggcgtgctg tctgcgccgt tgatcgaaat cgcaaccagc
     2941 gtgtccgcct gggcaaggaa gggacgggcg tctctgagcg cgcgggcggc gagccggctt
     3001 ccgtcccagc agatcccgat gcgctttgcc ttgaaggcgc cgcggaaaat gtgaggaatg
     3061 aacaacaccg gcccgccggc ctgaaacagg atctctcgcg gaatggtatt gtcgaaggtc
     3121 tcgcgttctg cctccggctg caggacgatc gcaagatcat agagccgcgc agcggcgccg
     3181 atcgagacag ccgcgttggc tgcgaagtcg gcgctcgggt ggctcgcgta ggcaatcccc
     3241 gcattgcgcg cctcggtttc gaagatcgag agcgctgtcg cggcgcgctc ggtggccctc
     3301 tcccgctcga cttcgaagac agccgcaacc gcagcggcgg cgcctccgtc ggggacatag
     3361 gccgcggccg tcgatatgta gccgattgca agcgcatcga gatgggcgcc gaagcttgcc
     3421 gccagggaga ccgacgcgtc gaccacgggc cgctgcggac gctctgtcgg aacgtttacc
     3481 agaatatcct tgatcatcgc gattccctcc gagaagcccg acgatggtct cagctttgcc
     3541 gggcgcgaat gttgacctga atcaagagtc gaagcggtgt tcatcacagt caggaattcg
     3601 acagaccggc gggcggcagc aagtaaagcg tcattgcgaa aatgacgtag agcggcgacc
     3661 gcctccaggc aaaccgctgg tcatgtcacg ccctgagctt ggcttcgtcg tcctcccagt
     3721 cattccactc gacattggtg acttccagat agcgcgaaac ggccgagccg atggtcggaa
     3781 agaagtaact ctcgccgagc tgcgcataca atccgaatct cttcagcttg tccttgacgg
     3841 ggtccttgag ttcggcaaag caaagctcga tccctcgctc gtgcagcgct acgtccaact
     3901 cggcgacggt gtcgcaggcg gtgacgtcga cgctggtgac gggctcggcc gcaaccacca
     3961 gccagcgtac gggcgtcggc gatttcgcga ctgcatccag gacgcgttct ctgaagaact
     4021 cggcattggc aaaaaacagg ggtgcatccc accggaacag gaccagtccg ggaatccggc
     4081 gtgcatcagg atatcgcgtg atgtcatgat agcccttgac gccgtaggcg cggcccagca
     4141 cggcggagtg ggggcgccaa ccgtcccaca agaactccgc gatggcgaag gcgatcgcca
     4201 gaccaatccc ggggatcgcc ccgaacacgg cgacgccgac gaagcagagg atcgtcaacc
     4261 agaactccca gcgctggatg cggtagatcc gtttgagatc ggcgatctcg aacaggccga
     4321 ttgcagatgc aatgacgacc gcagccaacg cggcgctggg caaatgccgg aggaggttcg
     4381 gcgccaggac aagaagaaaa gcgatggcga gcgcgccgac gacgctggtc agttgggagc
     4441 gcgcgccggc tgtctccgca acgggagtgc gcgaggagct gctgctgatc gggaagccct
     4501 gaaagaagcc ggttgcgaga ttggccgcgc cgagtccgac catctcctgg tttgggtcga
     4561 cgtcatcacc caatcgcgcg gcataggttc gcgaaagcac gctggtgtcg gcaaatgaga
     4621 cgagcgcgat cgcgcacccg ccgatcaaca ccggaacgag atcggtgtag ccgatccatg
     4681 gaacggcaaa cgccggaagc ccctgaggca gcggccccag gaccttcacg ccgtaacggg
     4741 cgctgagatc gagcaggccg acgatgacgg ttgccccgac gactgcaatc aggatgcccg
     4801 gcagccgctt gctgttccgg agcagcagga tgacgatcaa tgtgccgagc ccgacggcaa
     4861 aggcgaccca gtttgtcttt ccgtcgtgga tggcccgggc gatcgcccag aggtcgcgca
     4921 agggtccttc gctctcgatg gagaagccga ggagcttggg cagttggctg atcaggacgg
     4981 tcagtgcaat gccgtttata tagccgtagc ggatcggctt ggagagaagc tcggtcacaa
     5041 agccgaggcg cgcaaggccc gccagaatga gtactgtccc cgaaacaagg gccatcatgg
     5101 cggcgagcgt tgccgcgcga ccgggatccc cgccggccaa cggcatgacg acgccgagga
     5161 tgacggcagc cagcgccgag tctggtccga gaacgataat gcggcttggg ccgaacaccg
     5221 cgtaggccaa cagcggaacg atcgtcgcat agaggccgtg gatgccaggc aagcccgacg
     5281 ccgccgcgta ggcaattccg actgggacca gcatcgtcgc cagcacgagc ccggcgaaga
     5341 tgtcatgcgg cagccacgtc gcctcatatc ggcggaaagt gtcgacgcct ggcagccagc
     5401 ggatcaagtg ccgtcaccag ccgtagtaac tttcacgacg accacggcaa cacttgcgaa
     5461 aaagaccttc agaacactca tgctttcccc tccacctcac tctaccgcat tcgagccgcc
     5521 tgcaattgat gggaacgaga atatcgtcag cattttgcgg tgtctggcgg cgagccttga
     5581 tccagctcaa gctgatcttt ggtcctggac cctcgcggca cgtcggcatg cgatcgggta
     5641 cgcctcaact caaggcagac gtcgcgggtg tctgcagcaa cgaggcgcac agcaggagcc
     5701 gccgccgatc tttgctcggt ctcgtcaaga cgatcgttat cgatttcagt tcgaaagtac
     5761 ggctgctccg tatggcacct taggacaatt gccggcttga tacttctttg gcttgaatag
     5821 gcgggttccg tgtatttcga cccccttgca cgggacgcgc ctctccaaag gagagtccat
     5881 ggcggccccg gttttgtgcc cacgaagctg ggaccgcccc gcggcgccaa gcaaggattt
     5941 tagcgacgaa gggtcgcagt cgggacacct gtttgccctt ggcaggaagg gtaaccagca
     6001 tgacgaggct tatcgagtac aacgcacgcg ctgccctgtg ccgccggctc gcgaaacttg
     6061 agcctgtgag cagggatata tggctggcgg aggccgagcg atggacgcgc ctcgcacaag
     6121 ccagtatggg cgcgcaagac aacgaagacg gggctgaggc gccggaaggc gcaagccagt
     6181 cgctgggcga tgcagtgaag gaagatgcag tgaagcaaaa agactgagag actgtggcgg
     6241 cgcgcggctg ccttcaggca agctgcagtg tcaaatgtct gacctgagga tcgctgcccg
     6301 atccggcgcg gaagccaaat ttgctgaaca ccttgagcat tgcggcgttc tccgggagaa
     6361 cctccgccgt caactccttc aggccggctt cgcgggcgat cacaagcaga tggcgcatca
     6421 ggatggtcgc gatccccttg ccttgatagg cgtcgaccac gacgaatgcc agttcagcct
     6481 gccccggccg gacgatgatg tagcgtcctc cgccggcgat cacggagtca ccgtcctcat
     6541 cgacatgcgc aaccagggca acatggttgc tgaaatcgat attgatgaaa aacgagactt
     6601 ctttgtcggt aaaaccgcgc ttagggacaa agaagcggcg ctgcagagat tgtgtgctgg
     6661 tccgaccgac ggcggcgagc atgtcttccc gatcgtccgg ctgcagggcg cggatctcga
     6721 tcgtacttcc gtcgcgcaga tgctcgacga cagaataatt tttcaccatg atcgcgctcg
     6781 tgaagatcga ttgccggtcg cagggtccca caaaggcagg gtcccacaaa gtaagaagtg
     6841 cgggggcggc agatgcttga tccagatcaa gcccatggcc ggagcagcgt ttgtagaaaa
     6901 ttgcgggtga attgcccggc aaggcccgcc ggaagcgggt gagcgcccgg aggggtagtg
     6961 gacatggcat tgcgcggctt tcggcagaac cgctcgagcg aggaggtgat ccagatcgcc
     7021 atccgcctcg gcctcctcgc cgcgctcatc gtctggtgct tcatcattct tcgtcccttc
     7081 attgccattc ttgcctggag cgcggttttg gccgtcgcgc tctatccgct ctatgcctgg
     7141 ttgtccgaac gtctcggagg gcggccgaag ctcgcggcct gcatcatcac ggtgattgcc
     7201 accgccatcg tgatcggccc cgtgacatgg cttgggttcg gtctggtcga ggggttgcga
     7261 acgatctacg agcaagccga ttcggaaatg ctgcaggtgc cttcgcctcc cgcctcgatc
     7321 aaggaatggc cgctggttgg cgaacgaatc tacgctctat ggaatcaggc atcgaccaat
     7381 cttggagctt ttcttggcca gcttgcaccc tacgtcaaac caatcatccg ctcgatgctc
     7441 ggcatggcgg gcagtgtgag tttgggcgca ctcaaattcc tgatttcggt gatcctggcc
     7501 gggtttctgc tcgcgccggg accgcggttc gtgaccggaa tcaggaccat gatggcgcgg
     7561 gtcctgccgc agcgtagcga ggaacttgtt acgctgggcg gggcgacgat acgcacggtg
     7621 tcccaggggg tgattgggat cgctgtggtt cagtcgttcc tgttcggagt cgggctgaag
     7681 ttcgccggct tcgcccacgc cggcctcatc gcattcctgg tcttggtgct ttccatcgtg
     7741 caattgggaa cgagcatcgt tctgatacct tgcgtgatct ggatctggat gaccaaggat
     7801 accgcgacgg cgctgttcat ctccatctac ctggtgctgg tcggtctggc ggataacgtt
     7861 ttgaaaccgc tgctgatggg gcgtggtctg tccacgccga tgcccgtcat gttcctgggc
     7921 ttgctgggcg gaaccctcgc gcatggaatc gtcggcctct ttattgggcc gatcatcctg
     7981 gccgtcggct gggaattgat gatggcttgg gtgcgcgatg agcgcacaga ggatgttcca
     8041 gctcccgcgg agcgcgttcc tgccgaaatt tgaagcatct gcgccggctc gaatagcaaa
     8101 ataccgctcg cccgcgaccg cctgcgccct tgacctatgt caaagggtca gccccggccg
     8161 gggccatgct cagccccaaa ctccgacagc gatggattcc acacatgtcc cttgacaaac
     8221 agacggtctc ggacggcgtt ccgccctcat tctttacgtc cgccctcgaa taccttgtcg
     8281 atgcggggca gcgcagcgtg ttgttcctgg acgtcatgcg ccaacgcggt gtccagttcc
     8341 gcgagcatat ggccgaaacg gtgcccaatg tcctcgacta cggcgtcgag ctggttgtcg
     8401 acggcagaaa tctcgaacga cccgtgaact acggattggt gcgaatagtg cctccaaagg
     8461 gcgtcgagat cgatctgaag cgccgtccgt tcgtcgtggt cgatccgcga gccggtcacg
     8521 ggcccgggat cggcggcttc aaggccgaca gcgaaatcgg cgtggcaatg aaagcggggc
     8581 atccctgcta tttcgtcggc ttcatgcctg aaccgatgcc cggccagacg atccaggata
     8641 ttgcgcgcgc cgagggggtg tttctggaga aagtcatcgc gctgcaccct caagccgacg
     8701 gcaagccctg cgtgatcggc aactgccagg ccggctgggc ggtgatgatt ctcgcttcgc
     8761 tgcggcctga attgttcggc ccgatcatca tcgctggagc gcccttgtct tattgggccg
     8821 gcgtccgcgg caagaacccc atgcgttata cgggcggcct tcttggcggg agctggctga
     8881 cggcactcac cagcgatctc ggcgccggca agttcgacgg cgcgtggctg gtgcagaact
     8941 tcgagaacat gaacccctcg aacacgctct ggaccaagca gtacaacgta ttctcgaaaa
     9001 tcgataccga agcgccgcgc tatctcgaat tcgaacggtg gtggggcgga cacgtgaatc
     9061 tgaatgccga agagattcag ttcatcgtcg acgaactctt catcggcaat catctggcga
     9121 ccggccgtat caagacctcc gacggacagg tggtcgacct tcgcaatatc cactcgccgg
     9181 tcgtcgtgtt ctgctcgaag ggcgacaaca tcacgccccc gcagcaggcg cttggctgga
     9241 tcctcgatct ctatcatgat gtcgacgaga tcagggctta cggtcagacg atcgtgtaca
     9301 cggtgcatca gaccgtgggt catctgggaa tcttcgtttc gggtggcgtg gcgaagaaag
     9361 agcacagcga attttccagc aatatcgatc tgatcgatgt gttgccgccc gggctctatg
     9421 aggcaacctt cgaggaaaga tccgccgata ccaccaaccc ggatctgttg tcgggaaaat
     9481 ggatcatgcg ttgcgaacgg cgcacgctcg acgacatcag ggcgatggga ggcaacagtc
     9541 tcgaggacga gcggcggttt gcgactgccg ccaaggtgtc ggagatcaat ctcgccgcct
     9601 accgaacgtt cgtgcagcca tggatcaagg ccatggcaaa tccgcaaatg gccgagttgg
     9661 cgcgcaagtg gcatccgctc cgcgtgcaat atgaagcgct cgcgggacaa aacccgctga
     9721 ccaaggcgat cgagaccacc gccgacaagg tccgcgagaa ccgcaagccg gccgccaccg
     9781 ataatccatt ccttgcgctt caggagaccg tttcgaagag catcgttgga gcgcttgatc
     9841 agtggcgtga cgcccaggaa gcgctcagtg aaaggctgtt tctctcggtc tatggttcgc
     9901 cggcacttca ggccgcggtc ggaatcgatc cggaagcgga cccgtcaccc atgcccgaga
     9961 tgtcgcagga ctaccacaaa cgtctcgaag cacgcattgc ggaattgaaa tcacgcattg
    10021 ccagcggcgg cttgagagag tgcgtcgtcc gcggtctgct ctacgtcggc gcttcgcgcg
    10081 gcatggtcga tgagcgaagc ctcgaggcgc tgcgtcgggc gcggacgaat gacagcggat
    10141 cgcggctgac acttgccgag ttcaaggcga tggtgcgcga gcagttcttc atgctgctgc
    10201 tcgatcagga ggccagtctt gcagccattc cgaagctgct gccgaaggat gccaaggcgc
    10261 gccgtgccgg gctcgcggtg atccgcgagg tgctttccgc aagcgcggag atttccggcg
    10321 aaaccgccaa ccgcctgcgc cgcgtgaccg aactgtttgg aggtaccgaa gacagcgcgt
    10381 cgcaggcgag tgcaaaccct ttagatcccc aagcgaaggc ctcataaccc ggagccagac
    10441 gatgagcgca caaccggcag gcgagacacg acctcattcc aagtacgatc gcctgattgc
    10501 ggcggcaaag gccgtcccca ccgtcacgac gatcgtggtg catccctgcg acgagacctc
    10561 gctgcgcggc gctatcgagg ccgcagaagc cggaatcatc aagccgatac tcgtcggccc
    10621 cgccgcgaag atcgcggccg cggccgccaa gcataacatc aacatcgccg gctacgagat
    10681 tgtcgacgcc gagcatagcg aggcggctgc cgccaaaggg gtcgagctca ttcaccaggc
    10741 caagggcgag gtgctgatga agggcagcct gcacactgac gagctcatgc gcagcgtgac
    10801 ggcgaaggca accggactgc ggaccgaccg gcgcatcagc cacgtcttcg tgatggatgt
    10861 gccgacctat accgataccg ttttcattac cgacgctgcg atcaacattt tcccggatct
    10921 cgacggcaag cgcgacatca ttcaaaacgc gatcgatctg ttcaaccagg cggggttcgg
    10981 cacggttccg cgcgtagccg ttttgtccgc cgtcgaaacc gtgacctcca agattccgtc
    11041 gaccatcgag gctgcggcgc tttgcaagat ggccgatcgc gggcagatca ccggcggtct
    11101 gctcgatggt ccgctcgctt tcgacaatgc gatcgacgtc gaagccgcga ggatcaaggg
    11161 catcaagtcg gaagtcgccg gacatgccca gatactggtg gtgcccgatc tcgaagccgg
    11221 caacatgctg gcaaagaacc tcgcatattt cgcgaaggcc gatggggcgg gcgtcgttct
    11281 tggtgcccgc gttccaatcg tgctgacctc tcgtgcggac tcgccgagag ctcgcatggc
    11341 ctcttgtgcg gtggcggccc ttcaggcaga tgcgcgtcgc cgccgcgccg tcaacgctgc
    11401 agcgtgatcg ccatggatac gatcctcgtc gtcaacgcag gttcctcgag cgtgaagttt
    11461 caggtcttcg cggtcgacgg agatggcagg ctgcaacggc agctcaaggg acagatggat
    11521 gggattggga gccggccgcg gctccgtgca accggagcga agggcgacac gctggccgac
    11581 cgcgcctatc cgatcgaggc ggttgctgat gtcgcggcgg gattgatggt cgcgggcgcc
    11641 tggttgcggg acgaactccg catcacgccg atggccgtcg gacaccgcgt cgttcacggc
    11701 ggtcccgact acgatcggcc ggttctcgtc gatcacgggc tggttgcgcg cctggagcgc
    11761 tacgttgcgc tggccccgct tcatcagccc tacaatctcg cgccgatcaa atcgctgctt
    11821 gccaaccttc cggcgctgcc gcaggtcgca tgcttcgata ccgcctttca tcgcggccac
    11881 gacggggtcg ccgatcagta cgcaattccc cgtcagcttt acgacgaggg agtcaggcgt
    11941 tacggattcc acggcctctc ctacgaatat atcgccagga ccttgccgaa ggttgcgccc
    12001 gacatcgcgg cgggccgcgt gatcgttgcg catctgggca gcggcgcctc gatgtgcgcc
    12061 ttgaagagcg gccgcagtgt cgaaagcacg atgggcttca ccgcgctcga cggcttgccg
    12121 atgggaacgc ggccggggca gatcgatccc ggcgtcgtgc tctatttgct gtcggaaaag
    12181 cggatggagc ccggaaaggt gcaggacttt ctctaccgcg attgcggatt gaagggcctg
    12241 tccggcgtca gcaacgacat gcgcgaactg ctggagagcc gcgatccgcg tgcagccttt
    12301 gccatcgact atttcacgta ccgggtaggg ctttgtgccg gcatgctggc ggctgcgctt
    12361 cagggcctcg atgccttcgt ctttaccgca gggatcggcg agaattcgga tgccatccgc
    12421 tcccgtgttg ccgagaggct ggcatggctc ggtgtttccc ttgacgccgc cgagaacgcc
    12481 aagcgctccc tgaaaatatc gcagagcggc agccgcattc ccgtttatgt cgttccgacc
    12541 gacgaagaac gaatgatcgc cgaacatact ctgtcggtga tttggggcgc aaactcaaaa
    12601 gccaaacgcg agatcgcatc atgagcattc cggaatatcc cgagacaaag gtcgcgctga
    12661 agggaaaaaa gggacttatc gtcggcatcg ccaacgatag gtcgatcgcc tggggatgcg
    12721 ccaaggcctt tcgcgccttg ggcgcggacc tcgcagtgac ctatctcaac gatcgcgcaa
    12781 aaaagcacgt cgagccgctc gcgcagcaac ttgaagcgca gatcttcgtg ccgctcgacg
    12841 tcagcgtcga gggtcagacc gaacaggtct tcgaacggat cgcgaaagag tggggccagc
    12901 tcgacttcct gctgcactcg atcgccttct cgcccaagga ggcgctgcac gggcgcgtga
    12961 tcgacgtcgg gcgcgacggc ttcctcaaga ccatggacat atcgtgctgg tcattcatgc
    13021 gcatggcg
//
LOCUS       DATLJT010000635         5113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_38477,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000635 DATLJT010000000
VERSION     DATLJT010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38477"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VM910_14695"
     CDS             <1..560
                     /locus_tag="VM910_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI63814.1"
                     /translation="GGHVSICAPIDMAMGHGMPAIQEALDHGLRPSLSSDHGVTIAQD
                     FFTVMRSTFTLQRLLILQRARKGEQNLPPLLSCRDVLEFATIEGARCANLADRIGTLT
                     PGKEADIVVLRADRLDVWPLNNAAMTVVNMMNPSHVETVFIGGKVKKWRGSLVGVDVP
                     SVLRLVEEARDGVLRRANYAKNLLG"
     gene            complement(1111..1659)
                     /locus_tag="VM910_14700"
     CDS             complement(1111..1659)
                     /locus_tag="VM910_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVI63815.1"
                     /translation="MARPKTVFESRDVSAFNPQEPAAKPELRRLRPGPMRPPLGLPVH
                     RSVVPEGGEAPEVLPDLAKETEGAPQFARMTADPFLDPQTREAINAVKRPVLVIAGFA
                     TEAVVLDAALSALENDYRVQVPVDACGGMSARTEDAALRQIEAAGGVTTSVVSLVTAM
                     APDFAREPGRNTFAALQALRLA"
     gene            2364..2939
                     /locus_tag="VM910_14705"
     CDS             2364..2939
                     /locus_tag="VM910_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI63816.1"
                     /translation="MGWVAALRAQAAAPETASDDMLIRRIAQGDQLAMRMLFGRHRVP
                     LYRWFLRLAGDEALAEDLLSDVFLDVWRQAATFESRSSVSTWLLAIARHKALSARRRR
                     PDAELDEKIASTVADPADDPEVVLQKKTRADLLRRCLARLSPEHGEVIDLVYFHGKSV
                     KEVAEIVGIAEATVKTRMFYARKNLAELVET"
     gene            complement(3247..3531)
                     /locus_tag="VM910_14710"
     CDS             complement(3247..3531)
                     /locus_tag="VM910_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63817.1"
                     /translation="MAVAVTKIDTAELGTVAYNCALTQRAHAGVSAVKSQMEVYRAKA
                     KEYARLTIHTKSLRESRRYRKLAEMYWALALGERSESVQPSKMIVKTSPT"
     gene            3592..4383
                     /locus_tag="VM910_14715"
     CDS             3592..4383
                     /locus_tag="VM910_14715"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI63818.1"
                     /translation="MARMPAEWRNQVAVVTGGARGIGRATARLLAQHGAAVCVNYAVQ
                     SDAAEGLVAEISTAGGRAIAVAADVADSAAVEAMVARTEAELGPVTILVNNAGVSWQG
                     TVDTYDPDQVARMRRVNVDGLIHATRAVRGPMRARRYGRIVNMASIAGIGTALSGNAF
                     YAATKGEVMILTRRFALELGQHGITVNAVAPGFVRTDLTQRGRGAADWPGTEERFAAR
                     AMMGRIGEPEDIANAVVFLASPESGWITAQVLTVDGGRMDYIGHN"
     gene            complement(4529..>5113)
                     /locus_tag="VM910_14720"
     CDS             complement(4529..>5113)
                     /locus_tag="VM910_14720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI63819.1"
                     /translation="HMALPAVRQAEMLADELEFAVRQHSPKSIALLGCAGGNGLDRLV
                     GTGVERVVGIDINPAYVEAVRSRFRSRIPGLELYVADIQCALPQLSPVDLLFAALIFE
                     YVDVAMTMSSLQTVCTPGGALVIILQAASSSLEAVSPSPYKSIRLLAPAMRLLDQADV
                     QNQAIAAGFSLASSRVVNLQSGKRFIVLSFRTEH"
BASE COUNT          958 a         1542 c         1624 g          989 t
ORIGIN      
        1 gcggcggcca tgtgtcgatc tgcgcgccga tcgacatggc gatgggccat ggcatgccgg
       61 cgatccagga ggcgctcgac catggcttgc ggccgtcgct gagctccgat cacggcgtga
      121 ccatcgcgca ggatttcttc acggtcatgc gttcgacgtt cacgttacag cggctgctga
      181 tcctgcaacg cgcccgcaag ggcgagcaaa acctgccgcc gctgctcagc tgccgcgacg
      241 tgctcgaatt cgccaccatc gagggcgcgc gctgcgccaa ccttgcagat cgaatcggca
      301 cgctcacgcc cggcaaggag gccgatatcg tcgtcttgcg cgccgatcgg ctcgacgtgt
      361 ggccgctcaa caacgcggcg atgacggtcg tgaacatgat gaatcccagc cacgtcgaaa
      421 cggtcttcat cggcggaaag gtgaagaagt ggcgcggcag tctggtgggc gtcgacgtgc
      481 cgagcgtgtt gcggctggtg gaggaggcgc gcgacggcgt cctgcgtcga gccaactatg
      541 caaaaaacct gcttggctga cgcgagcgtc gatagccaga accgacgttc acatcacttt
      601 tcagccgagt gaaatcgacg gagcggtcag ctcggcctgc ttcccaatcg tgctgaacca
      661 tgtctgcttg ccgaggcgaa gcggagctaa cgcggacggc gtcccccgat cagagcggcg
      721 tcaatcccgc gagcacgcaa gatcgtttcg agctccgtat gcggcttcgc caaaatgata
      781 tcgccggctt ggacgagact ttggggtgaa gtgcggccga tggtgccccg tcgtcgatca
      841 ctccctgtgc cactggaggc accgtctctc ccaggaggcc cacatctgaa tggtctgagc
      901 gcactgcatg ccgtgtccag atgatagtgc ttccggccgc ccgatagtgc gccgccagaa
      961 gattcagctt atcgacgacc tgcaaaccta gtggagcggc gatcggcgac ttctcgacat
     1021 tgcaattttg caggtcgacg gccacgaggg cacttttcct tgggtcaatc gcaaaagtga
     1081 ccattttgga tcctcatttg cttggggcgc tcacgctaac cttagcgctt gtagggcggc
     1141 gaaggtgttt ctaccgggct cccgcgcgaa atctggcgcc atcgcggtca cgagcgatac
     1201 gacggaggtc gtcacgcctc ccgctgcctc gatctggcgc agggccgcgt cctccgtgcg
     1261 cgcagacatt ccgccgcagg cgtcgacggg cacctgcact cggtaatcat tctctagcgc
     1321 cgaaagcgca gcgtcgagca cgacggcctc cgtggcgaag cccgcgatca ccagaaccgg
     1381 ccgcttgacg gcgttgatcg cctcccgcgt ctgcggatca aggaacggat cggccgtcat
     1441 gcgagcgaat tgcggcgcac cctcggtttc ctttgcgagg tccggcaaca cctccggcgc
     1501 ctcgccgccc tctggcacga cgctgcggtg cacgggcagg ccgagcggcg ggcgcatcgg
     1561 gccaggacgg aggcggcgca gctcaggctt tgcggcgggt tcttgggggt tgaaggccga
     1621 tacgtctctg ctttcaaaaa ccgttttcgg acgggccacc ggtaaacgca tcattgggca
     1681 cgacgcgctc cgtggtttgc cggctgcgct atcagtctga gttttagtcg acgtcccaat
     1741 agctcgggcg tcacatcggc cggagccgga accgcctgca cccttgcgcg ttacttctct
     1801 ggatgatgaa cggaaccgga ttaccggcag ttcatcccac aggccgctcg gctcaagctc
     1861 ccgggcggcc tttggttgtt ggttgcaccg ccgagaggca gtgtgcagtg cggttgcgat
     1921 ttacaggtca gtaccagtac gaactcccgc tctgttacca ctctggggca gcagctcggc
     1981 caccggcgca agcgctttga aggaaaacta acgccgttgt ccaacgttcc gtagtgggtg
     2041 gtgagcctcg ccccggttgc gggcgagccg ggattgatag cgtatgagtg ccccaactgc
     2101 gcgtatgtga cgagcgtgct ccagcagccg aaggacatgc attagtcgtg tgcaatgtca
     2161 ctaggtgctg gcgagcgggg tcatcgcggc acagagcatt cataagttcc atcgccgagg
     2221 tttaactgtt gcggccggaa aacagcaaat tttgacagtc gatcaatggg taagaagggg
     2281 tgcaaccttt gccccccggg gcgagtttgt agggcgggac gtgattacca ggggattatc
     2341 caatgaccgt gacctgtacc cgtatgggat gggtcgccgc attgcgcgca caggctgcgg
     2401 cgcccgagac tgcttccgat gacatgctga tccggcggat tgcgcagggc gatcaactcg
     2461 ccatgcgtat gctgtttggc cggcatcgag ttcctttgta tcgctggttt ctgcggctcg
     2521 ccggcgacga ggcgttagcc gaagacctct tgagcgatgt gtttctcgac gtttggcgcc
     2581 aggccgccac gtttgaatca cgctcgtcgg tttcgacttg gttgctggcg attgcccgcc
     2641 acaaggcgct ctcggcgcgg cgccgccggc cggatgcgga attggacgag aagatcgcgt
     2701 cgaccgttgc ggatccagcg gacgatccag aggtcgtgct gcaaaagaaa acccgtgcag
     2761 acctgttgcg ccgctgctta gcccgactgt caccggagca cggcgaggtc atcgacctcg
     2821 tctacttcca cggcaaatcg gtcaaagagg tcgcggagat cgtcgggatc gccgaggcca
     2881 ctgtcaagac gcgcatgttc tatgcgcgca agaacctggc cgagctggtc gagacgtagc
     2941 ggctaagcac gggatggtcg ggcattcagt ggcgacgacc cgtaagctcc ttcagaagcg
     3001 cggcgagacg ttccgggaca ggttggtcgg cggcactgta ctcggcccgc agccattgcc
     3061 cgagttcccg cagccatggc gttcgggcca ggatgtcccg acgttcggac tcgcccgacc
     3121 tacgctgctc gcgctggctc ctacggacac gcgcggctgg cggtaggctc tcacatgcgc
     3181 tcgacccctg ggcacgacgt acccacttct gctcagaacg tctatgaaat tctaagggat
     3241 aagtcgtcat gtcgggctcg ttttcacgat catttttgaa ggttgaacgc tctctgatct
     3301 ttcgcccagc gcgagtgccc aatacatttc cgcgagtttc cggtatcgac ggctctcccg
     3361 cagcgatttt gtatgaatgg tcagacgagc gtactctttc gccttggcac gataaacttc
     3421 catttgcgac ttcacggcag acactccggc gtgcgcccgc tgcgtcaacg cgcagttgta
     3481 cgccactgtt ccgagctctg ccgtgtcaat tttagtgacg gcaactgcca attgttcttg
     3541 cggctcttac aatgatctcg cccgcctccc gtcgaaagct cgcgatacca aatggctcga
     3601 atgcctgctg aatggcgcaa ccaagtggcg gtggtgacgg gcggcgcccg cggcatcggc
     3661 cgagcaacag cgcggctcct ggcgcagcac ggcgctgcag tgtgcgtgaa ctatgccgtg
     3721 cagtccgacg cggctgaggg gctagttgct gagatttcga ccgcgggcgg acgcgctata
     3781 gcggtagcgg cagacgtagc cgactcggcc gcggtcgagg cgatggtcgc acgcaccgaa
     3841 gcagagctgg ggccggtgac aatcctggtg aacaacgcag gcgtgagttg gcagggcacg
     3901 gtggacacct acgacccgga ccaggtcgcg cgcatgcgcc gagtaaacgt ggacggcttg
     3961 atccatgcga cgcgcgcagt cagggggccc atgcgggcgc gacgctacgg tcggatcgtc
     4021 aatatggctt cgatcgcggg gatcggcacc gcgctatccg gcaatgcatt ctacgcggca
     4081 acgaagggcg aggtgatgat tctcacgcgg cgctttgccc tggagctggg ccagcacggc
     4141 atcaccgtga acgccgtggc gccgggcttt gtccgcaccg acctgacgca gcgcggtcgc
     4201 ggcgcggcgg actggccggg cacggaggaa cgcttcgccg cacgagcgat gatgggccgc
     4261 atcggtgaac ctgaggacat cgccaatgcg gtggtgttcc tggcttcacc ggagtcaggt
     4321 tggattacgg cgcaggtgct gacggtggat ggtggacgga tggactacat tggacacaat
     4381 tagatcgagc gccaagcaga tggcgtcgag atgagacaag ccgtgcgtcc gctcatttcc
     4441 ggttcgcctc cgaagcggac gtaacttgat cgcagcgctg tgtgtcgcga agtgtcctcc
     4501 agcttccggc gcatcgatca gccgggactt agtgctctgt ccgaaaggac aagactatga
     4561 aacgcttccc cgattgcaga tttacgacac gagatgatgc tagcgaaaat cctgcggcaa
     4621 tggcttgatt ttgcacatcc gcttggtcca ggaggcgcat cgccggagca agcaaacgga
     4681 tactcttgta aggcgacggg gataccgctt ccaaactcga actcgcagct tgcaagatga
     4741 tgaccaaagc accgcccggc gtgcagaccg tctgcaagct gctcatagtc atggctacgt
     4801 cgacgtattc gaatatcaac gctgcgaaaa gcaaatctac tggtgagagt tgcggcaatg
     4861 cacactggat gtcggcaaca taaagctcga gcccggggat tctcgagcgg aaacgactgc
     4921 ggaccgcttc tacataggcg gggttaatat cgattccaac aacccgctcg acccccgtcc
     4981 caactaatcg atctaaaccg ttgccgcctg cgcacccgag tagggcgatc gatttcgggg
     5041 aatgctgtcg tacagcgaat tcaagctcgt ccgcgagcat ctcggcctgc ctgactgccg
     5101 gcagcgccat gtg
//
LOCUS       DATLJT010000636         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_268543,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000636 DATLJT010000000
VERSION     DATLJT010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268543"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..528)
                     /locus_tag="VM910_14725"
     CDS             complement(292..528)
                     /locus_tag="VM910_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63820.1"
                     /translation="MPATSGTTASLPQIEVARVKPVDQVPPNVRLSDDLIDLMKAFSL
                     LSAEGIGRAWGAPLRPPTPFDERFGQWETRLWPQ"
     gene            complement(609..1610)
                     /locus_tag="VM910_14730"
     CDS             complement(609..1610)
                     /locus_tag="VM910_14730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63821.1"
                     /translation="MQFNELRRREFITLLGGAAAAWPLAALAQQGNAPRPLVACLFGG
                     SKASVRRNFDGFLQGMRELGYVEGQNWVFEARYADGDQARSPRLAEELVRLKPEVIAV
                     GTMAGVLGFKKLTDTIPIVSPVLIDPVGFGLAASHARPGGNVTGLLLTVEDLPSKQLA
                     LAVEMVPGTRKIGLLMNPGNPTHAGRRDALEGAATTLGIELIALQARVPDHLHAAFET
                     LARHAVTMVLALDDTLFFSERKRIALLAAAARLPTMFGLRQNVEDGGLMSYGVELYES
                     WRRTAAFVDKILKGARAGDLPIEFPTKLELVINLTTAKVLGLDVPPTLLARADEVIE"
     gene            complement(1724..2101)
                     /locus_tag="VM910_14735"
     CDS             complement(1724..2101)
                     /locus_tag="VM910_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63822.1"
                     /translation="MVDEQREHRAKENIFAFLQRRSIEQRRSYLARGRRFVVLDVVQL
                     SKSWTTAVKNWLARKDRASEITMDDLTAELRLRGLEPPYGAVKQELATRFAGMDEATQ
                     RKAGRKFAREIGMFIRESDKSLH"
     gene            3567..>3786
                     /locus_tag="VM910_14740"
     CDS             3567..>3786
                     /locus_tag="VM910_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63823.1"
                     /translation="MTKKRRVAALAAAKGFRALTAPLAERLNSLVTQSDGIALSSPGK
                     LDDRFGYHCHCGIIAIDQTQLTDCIFKRR"
BASE COUNT          737 a         1178 c         1111 g          760 t
ORIGIN      
        1 ctgcctcaca gcgggctagg tccttggggc agacccgaat tgttctgaaa tcgatccagc
       61 cagaccagga tagcacatgg caagcggctc tacgcccatg gcgcatcggt cctgttagag
      121 ccgcgacagc tctacgacac aatgctgtca agtcaggcca gagcggagca ttttcagtaa
      181 tgcgctgaat ttctgccgct ccgtaatcgc agccttggtc cgctcgccgt gacggacctc
      241 cctctcgctt cacatcattc gaccaccatg ccggtcgcaa tcacgcttct actattgcgg
      301 ccataaacga gtttcccatt ggccgaaacg ttcgtcgaag ggggtcggcg ggcgcaatgg
      361 tgccccccag gcacgaccga tcccctcagc gctcaaaagg ctaaaggcct tcatcaggtc
      421 gatcagatcg tcgctcaatc gaacattcgg aggcacctgg tcgacaggtt ttactcgagc
      481 cacttcgatt tgcggcaagc tcgcggtggt accgctggtc gccggcatcg tcgccgactc
      541 ctgcgcatac gctgcaatcg accatgccgc agccatagcc ccaaataggc atatgaaatc
      601 acgccttttc actcgatcac ctcgtcggcg cgggcgagca gcgtcggcgg gacatcgagg
      661 ccgagcacct tggctgtcgt caggttgatc accagctcca atttggtcgg aaattcgatc
      721 ggcagatcgc cggccctggc gcccttcagg atcttgtcaa cgaaggcggc ggttcgccgc
      781 cagctctcgt acagttcaac gccgtagctc atcagtccgc cgtcctcgac gttctgacgc
      841 aggccgaaca tggtcggcag ccgtgccgcc gccgccagca gcgcaattcg cttccgctcg
      901 ctgaagaaca aggtatcgtc gagcgcgagc accattgtca ccgcatgtcg ggccaaggtc
      961 tcaaatgctg cgtgaagatg gtctggcaca cgagcctgca aggctatgag ctcgatcccc
     1021 agcgtagtgg ccgcaccttc cagggcatct cgccgacccg cgtgtgttgg attgccggga
     1081 ttcatcagca gaccgatctt gcgtgtgcct ggaaccattt cgaccgcgag cgcgagctgc
     1141 ttgctcggca aatcctcgac cgtcaacaac aagccggtga cattgccgcc gggtcgtgca
     1201 tggctggccg ctaggccgaa gccgaccgga tcgatcagaa ccggcgagac gatcgggatc
     1261 gtgtcggtca gctttttgaa accgagaaca cctgccatcg tgccgaccgc gatgacctcg
     1321 ggcttgagac gaaccagctc ttccgcaagc cgcggcgagc gcgcctgatc cccatcggcg
     1381 taccgggcct cgaagaccca gttctggcct tcgacgtatc cgagctcgcg catgccttgc
     1441 aggaagccat cgaagttgcg cctgacgctc gccttcgagc cgccgaatag gcaggctacc
     1501 aacggccgcg gcgcgttgcc ctgctgcgcg agcgccgcga gcggccatgc ggcagccgcg
     1561 ccgccgagca gcgtgatgaa ctcgcgccgt ctcaattcat tgaattgcat cgcgtcccgc
     1621 cagccatggg ccgaattgca ggatatcgaa ttggcggtga tcagtcagcg ggtgtctgtc
     1681 tgctgcgctg cgggatttcg atccggtcga tgaccgcttg ggttcagtgg agcgacttgt
     1741 cgctctcgcg gatgaacatg ccgatttctc gcgcgaattt tcttccagct ttcctttgcg
     1801 tggcctcgtc cattccggcg aagcgagtgg ccaactcttg tttgacggcg ccgtaaggcg
     1861 gttcgagtcc tcgcaatcgc aattcggctg tcagatcgtc catcgttatt tcactggctc
     1921 gatccttgcg agcaagccag ttcttgactg cggtagtcca gcttttgctt agctgcacaa
     1981 cgtcgagaac aacaaagcgc cgcccacgag ccagatagct ccgcctctgc tctatgctcc
     2041 tgcgctggag gaatgcaaag atattctctt tggcgcgatg ttcgcgctgc tcatctacca
     2101 ttccgctcac cttgttttag ttaggtcgca ccgaaccgtg atcgccgtgc ttggcaattg
     2161 gagcagcacg gagaacacct gggggaagtt aagtgtggaa gcgcttcagt caaagcccgc
     2221 gcttaggggc cgaggtgtcg acgctcaagt tcaatccaga cttcaggcgg caaatcgtac
     2281 ctatggcgat aaagatactc gaggatttcc ccctcgttgg gggctgacgg tcccaccacg
     2341 gcatgcacga acttacgcca tacggactgg taacgacctc cagctcgggc tttctcatcc
     2401 cgaaagtcaa cgctcgcggt cacgatcatg gcgatctcct gctcgtcatg ctgagcggtg
     2461 gtgcacacta gcgcggcctc tatccaaaag acgtgatggc catcacaaaa aagcgctttt
     2521 aacaccgcgc aatattgcgg gcatccggcc gctcgcccga ttggctcaaa atgaaaagcc
     2581 cttagtcgcg ctaagatggc tgcgcagcga gacggccacc actccccaag atgaggctag
     2641 gaagtcccgc gccggcgatg ggcaacgaga accccgccca gggcatggga ttcgggcgcc
     2701 gactgagcgg ggttccgaat ccctcggggg gaccttgggt tggggagtag gaggtacgcc
     2761 cgagggcgtt tacgagggta tgccgttgac cggcacgccg caaccttccg ccaaccatcg
     2821 gcgatgcaac gtgcgcgcct tgggcgacac atcgacgttg catcgtgggc cagcgcaaca
     2881 cacccgtccg cggccccgcg gaggcacacc tcggtgccgc ccgacccacc catgaacgcc
     2941 tccagcagac cgaaccaatt cggggccgtt tgtcgtctat cggacgaatg ggcagcggga
     3001 tcgtggcgag cagggcagaa gagtatcggc gtcgcgcaca acggtgctta gagatggcgc
     3061 gcgcgtttgg agaccgcgac gcgcgggtca ccctagccca catggcgcaa ggcgtggctg
     3121 cggttggcgg ataattgcca agacgctaag aaggtccggc ctgcagtcca gcagcaacag
     3181 cagattcagc ccaaggacgg cgaccgagag taagccgcgc ctcgcttggc ggctcgacta
     3241 ctggcaggac attgccgacg gtggtcgccg acatctagaa attactgcac tcgcggtgtt
     3301 atgcattggc gttaggccca ggaaagagcg ttggctgcgt tggcgttgca tatcatgtct
     3361 cgtgctcggc ttagagagct tgtggtgctg tttgcgatta gctcggcact aacgctcgcg
     3421 gtatttttcc ttgcgctctg gctgctgccg tttgagacgt ttactcggtt tgctttgccg
     3481 cgatttcctg cgctgttcaa ctgatcgcac agctcgtcgc gacgcgagcc tgccatgaca
     3541 gcatttacaa aaagctgtcg gcggggatga ccaagaaacg ccgcgtcgcc gcacttgccg
     3601 cagcaaaagg ctttcgggcg cttacagccc cactagcgga acgattgaat agcttggtca
     3661 cgcaatcgga tgggatcgcg ctctcctcgc ctggcaagct cgatgatcgt tttggctacc
     3721 attgtcactg cgggatcatt gcgatcgacc aaacgcaact gacagactgc atcttcaagc
     3781 gccgct
//
LOCUS       DATLJT010000637         8362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3485037,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000637 DATLJT010000000
VERSION     DATLJT010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485037"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1995
                     /gene="dxs"
                     /locus_tag="VM910_14745"
     CDS             46..1995
                     /gene="dxs"
                     /locus_tag="VM910_14745"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HVI63824.1"
                     /translation="MQNWNIAVTSFSKTPLLDTIRTPDDLRKLKVEQLGQVADELRQE
                     TIDAVSVTGGHFGAGLGVVELTTAIHYIFDTPRDRLIWDVGHQAYPHKILTGRRDRIR
                     TLRTGGGLSGFTKRSESDYDPFGAAHSSTSISAALGMAVARDLSGGKNNVIAVIGDGA
                     MSAGMAYEAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGKTYRTLREVA
                     KQINKRLPKIIANRANRVEEYSRGFMMDGGTLFEELGFYYVGPIDGHNLDHLLPVLRN
                     VRDMETGPILVHVVTQKGKGYGPAEASADKYHAVVKFDVATGAQAKAKPNAPAYQNVF
                     GQSLVKEAHKDKTIVGITAAMPSGTGIDIFNKTFPERTFDVGIAEQHAVTFAAGLATE
                     GYKPFCAIYSTFLQRGYDQVVHDVAIQSLPVRFAIDRAGLVGADGATHAGSFDNAYLG
                     CLPNFVIMAASDEAELVHMVATQVSINDRPSAVRYPRGEGRGVELPEVGVPLPVGKGR
                     IVRQGSKIALFSFGTRLAECEKAAEELAALGLSTTVADARFMKPLDVDLLLKLAREHE
                     ILITIEEGAIGGFGSHVMQTLTDHGMLDSGMLKARSMVLPDVFLDHDSPNAMYARAGL
                     DAKGIVAKVFEALGRDFKTEAVKLA"
     gene            2160..2711
                     /locus_tag="VM910_14750"
     CDS             2160..2711
                     /locus_tag="VM910_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HVI63825.1"
                     /translation="MKIYLAGPDVFLPDRIEIGRRKTAICARYGLTGLYPLDHPVDLA
                     ADTISLSIFRGNEAMMDAADAIIANLTPFRGPGADAGTAYELGYMAGRRKLCLGYCND
                     PAAYADRVRRLTDVKPDGDRLVDPEGLTVEDFGLPDNLMLIHALDLYGCPLVAPKQAP
                     ADLWHDLTAFEICVRAAAERLAA"
     gene            2738..3472
                     /locus_tag="VM910_14755"
     CDS             2738..3472
                     /locus_tag="VM910_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HVI63826.1"
                     /translation="MSPAGKRVDVLLVERGLFESRARARAAIEAGLVTANDRQIVKPS
                     EVLPADADLQAQPAHPYVSRGGVKLAGALEQYPIDIEGHVCLDVGASTGGFTEVLLAN
                     GAELVFAVDVGRNQLHRSLRDHPRIVSMERTDIRKFEGKRLPARPDVVVIDVSFISLK
                     AVLPVALSLAAAPMHLLALIKPQFEVKGAHGKGGIIRDTAVHQQVCDDIAAFAASLGC
                     TDIRVFPSSIAGGDGNVEFFIGARRG"
     gene            3465..4706
                     /locus_tag="VM910_14760"
     CDS             3465..4706
                     /locus_tag="VM910_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HVI63827.1"
                     /translation="MVEYLEIDHVGHRGDGVALAADGQAIYVPYALAGETVEVAEVHG
                     HPDRRRLLAVKTASEARIAPFCPHFGVCGGCAIQHWREDAYRAWKRDIVIETLKQARL
                     DCEVTPLIDAHGLGRRRVTLHARLGTHEVLKVGFAAASSHDIIPVDHCPILDPALSGA
                     LDAAWALAEPILKNGKPLDIQMTATNNGLDADVRGSGPLAPPVIATLSGIAGKHRLAR
                     LTRHGELVLMRASPTVTIGTAEVTLPPGSFLQATTAGEETLAALVAERCGKARQICDL
                     FCGVGPFALRLATRARVSAFDSDAGAISALQKAATATRGLKPVSAETRDLFRRPLVPQ
                     ELRDSDVVIFDPPRQGAQAQAQQLAASKVPVVIAVSCNVATFARDARILLDGGYRMET
                     VTPVDQFRHTPHVELVARFSR"
     gene            4941..5912
                     /locus_tag="VM910_14765"
     CDS             4941..5912
                     /locus_tag="VM910_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63828.1"
                     /translation="MRLVLQARSNPLAWWWGLLALVSSVNIAFWFLLYREFCNSPAGS
                     SASNSDIGLMLLLCAGYVFGCAFRSFLPRADVQRICLFDTWLSSVVVGRSVATVAELC
                     FAAQWAIILRQLGEIAGAETTLNAAWLVVPLILVAECFSWYAVLTTNYLGNAIENSIW
                     AVAFLIVGVALCRLLPEFNGAVRWALVIAISGIAFYLAFLMTIDVPMYLGRWRADLAD
                     GSRLLGIFEGLRDISTRWIVTHDFAHWKDEIAWMSLYFSAAVWASLALCACYSLEDYL
                     PHYRTEPTALTARDMFGQRVAAQEAVAFPLRIAEASILLSAPFTPRR"
     gene            complement(5896..6117)
                     /locus_tag="VM910_14770"
     CDS             complement(5896..6117)
                     /locus_tag="VM910_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63829.1"
                     /translation="MSSNQGAVFYPEDLSLLGQVLDRAVQSLPPKARTPHNRAVLARR
                     IVACAVTGERDPVELELAALIDVKITAAA"
     gene            6238..6414
                     /locus_tag="VM910_14775"
     CDS             6238..6414
                     /locus_tag="VM910_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63830.1"
                     /translation="MQPIARTDTDIARGGSGIKRRALIAGLARAAKLPPGLISCGPDL
                     LVYTLLNHADQVID"
     gene            complement(6424..6957)
                     /locus_tag="VM910_14780"
     CDS             complement(6424..6957)
                     /locus_tag="VM910_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVI63831.1"
                     /translation="MPPTSNVSAANGLNVIRCFAIALAAALLAGCAAQSSVASRKSEF
                     VATRQVAIVRHRETRTAAVEHTSIVRRHDPYASEKDTGDLKLPSQGVASFYSDTRTAS
                     GEKFNPDELTAAHKTLPFGTKLRVTNTATGRSVTVRVNDRGPYVHGREVDVSYSAAQA
                     LGMVGSGIAKVKLDVVE"
     gene            complement(7165..8016)
                     /locus_tag="VM910_14785"
     CDS             complement(7165..8016)
                     /locus_tag="VM910_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI63832.1"
                     /translation="MRSLASIGAVLIAGALVAGGVAVVAAPGPTYKAEQHEAEKDAAN
                     AVDVELVLAVDVSYSMDMDELAIQREGYAQAIISKEFLQALKSLPNGKVSITYFEWAA
                     SSDQKIIIPWRLIDGPETADAVASEIMKTPVRRASRTSISGAIYFAMPLFDQDPYHGT
                     RRVIDISGDGPNNNGAPVTPARDEAVSKGIVINGLPIMVKEPSYSTMDIDNLDWYYED
                     CVIGGPGSFVVPIKDREKFKEAIRTKLLLEVASRTPERRLVPVAEKESRVSCLIGEKI
                     WQDRWGR"
BASE COUNT         1503 a         2707 c         2551 g         1601 t
ORIGIN      
        1 tgccgaaact gtgcaaccca tctcacgtcg gaacgttcct ccgacatgca aaattggaat
       61 atcgccgtga cctcatttag taaaacgccg ctactcgaca ccatcagaac gcccgacgac
      121 ctccgcaagc tcaaggtgga gcagcttggt caggtcgctg acgaattgcg ccaggagacc
      181 atcgacgcgg tctcggtgac gggcggccat ttcggcgccg gcctcggcgt ggtcgaactc
      241 accaccgcca ttcattacat tttcgatacg ccgcgcgacc gcctgatctg ggacgtcggc
      301 caccaggcct atccgcacaa gatccttacc gggcgccggg accgtatccg cacgctgcgc
      361 accggcggcg gcctgtcagg tttcaccaag cgctccgaaa gcgattacga cccgttcggc
      421 gccgcacact cctccacctc gatctcggcg gctctcggca tggccgtggc gcgcgatctc
      481 tccggcggca agaacaacgt gatcgccgtg atcggcgacg gcgcgatgtc ggctggcatg
      541 gcctatgagg ccatgaacaa tgcgggcgca atgaactccc gcctgatcgt catcctcaac
      601 gacaacgaca tgtcgatcgc cccgcccgtc ggcgcaatgt cggcctatct gtcgaggctt
      661 tactccggca agacctaccg cacgctgcgc gaggtggcaa agcagatcaa caagcgactg
      721 ccgaaaatca tcgccaaccg cgccaaccgc gtcgaagaat attcccgcgg cttcatgatg
      781 gacggcggca cgctgttcga ggagctcggc ttctattatg tcggtccgat cgacggccat
      841 aacctcgatc acctgctgcc cgtgttgcgc aacgtgcgcg acatggagac gggaccgatc
      901 ctggtccacg tcgtgacgca aaagggcaag ggttatggtc cggcggaagc ctccgccgac
      961 aagtaccacg ccgtggtcaa attcgacgtc gcgaccggcg cacaggccaa ggccaaaccg
     1021 aatgcgccgg cctatcagaa cgtgttcggc caaagcctcg tcaaggaagc tcacaaggac
     1081 aagacgatcg tcggtatcac ggcggcaatg ccctcgggca ccggcatcga catcttcaac
     1141 aagactttcc ccgaacgcac cttcgacgtc ggcatcgccg agcagcacgc ggtgaccttc
     1201 gcggccggcc ttgcgacgga gggctacaag cccttctgcg cgatctattc caccttcctg
     1261 cagcgcggct acgatcaggt cgttcatgac gtggcgatcc agagcctgcc cgtccgcttt
     1321 gccatcgacc gcgcaggcct ggtgggcgcc gacggtgcaa cccatgccgg ctccttcgac
     1381 aatgcctatc tcggctgtct gcccaacttc gtgatcatgg ctgcctccga cgaagccgag
     1441 ctcgtgcaca tggtcgcaac ccaggtctcg atcaatgacc gtccgagcgc ggttcgctat
     1501 ccgcgtggcg aaggccgcgg cgtcgagctg ccggaagtcg gggttccgct tccggtcggc
     1561 aaggggcgca tcgtccgcca gggcagcaag atcgcgctgt tctcgttcgg cacccgcctc
     1621 gccgagtgcg agaaggcagc cgaggagctc gcagcgcttg gtctgtccac caccgtcgca
     1681 gacgcccgct tcatgaagcc gctggatgtg gacctgctcc tgaaactcgc ccgcgagcac
     1741 gaaatcctga tcaccatcga ggaaggcgcg atcggcggct tcggttccca tgtcatgcaa
     1801 acgctgaccg accacggcat gctcgacagc ggcatgctga aagcacgctc gatggtcttg
     1861 cccgacgtgt tcctcgacca cgattcgccg aatgcgatgt atgcgcgcgc cggtctggac
     1921 gccaagggca tcgtcgcgaa ggtgttcgag gcgctcggca gggatttcaa gaccgaggca
     1981 gtgaagctcg cctagatcgc catcgcgctc tggtttattg tttgagcatg atctccgcgc
     2041 aaacgcgttc cgcgtttgtc gcgaggtaaa accggtgctc acttttccgg atcatgctct
     2101 aaggcgatcg gctacgagct ctcgcgaccg cttcgctcaa tctcaacgtc cggtgactca
     2161 tgaagatcta tctggcagga ccggacgtgt tcctgccgga taggatcgag attgggcggc
     2221 gcaagaccgc gatttgcgct cgctacggcc ttaccggcct ttatccgctc gaccatcccg
     2281 tcgacctcgc ggccgacacc atctcgctct caattttccg cggcaacgaa gcgatgatgg
     2341 acgctgccga cgccatcatc gccaatctca ccccatttcg cggccccggc gcggatgccg
     2401 gcaccgctta cgaactcggc tacatggcgg gtcgaaggaa gctttgtctc ggctattgca
     2461 acgaccccgc cgcctatgcc gaccgcgtgc ggcgcctcac ggacgtgaaa ccagacggcg
     2521 accgactggt tgatccggag ggactgacgg ttgaagattt cggactcccg gataatctga
     2581 tgttgatcca tgccctcgat ctctacggct gccctttggt cgcaccgaag caggcgcccg
     2641 ccgatctctg gcatgatctg acagcgttcg aaatttgcgt tcgcgcagcc gccgagcggc
     2701 tcgccgccta gcgccggtcc cgcgggagtg ctcgctgatg tcgcctgccg gtaagcgcgt
     2761 ggatgtgctc ctcgtcgaac ggggcctgtt cgaaagccgg gcgcgggcgc gggccgcgat
     2821 cgaagcaggc ctcgtcaccg ccaacgacag gcagatcgtc aagccgtccg aagtgctccc
     2881 ggccgatgcc gatttgcagg cgcaacccgc gcatccctac gtctcccgtg gcggcgtgaa
     2941 gcttgccggc gcgctggagc aatatccgat cgacatcgaa ggccatgtct gcctcgacgt
     3001 cggcgcctcg accggcggct tcaccgaggt gctgctcgcc aacggcgcgg aactcgtgtt
     3061 tgccgtcgat gtcgggcgga atcaactgca tcgctcgctg cgcgatcatc ccaggatcgt
     3121 ctcgatggag cggaccgata tccgcaagtt cgaaggcaag cgcctgcccg cgcggcccga
     3181 tgtcgtcgtc atcgacgtca gcttcatttc gctgaaagct gtgctgccgg tcgcgctgtc
     3241 gctcgcggcg gcacccatgc acctgctcgc gctgatcaag ccgcagttcg aggtgaaggg
     3301 cgcccatggc aagggcggca tcatccgcga taccgccgtg caccagcagg tctgcgacga
     3361 cattgcggcg tttgccgcct cgctcggctg caccgacatc agggtcttcc cgtcgtcgat
     3421 cgccggcggc gacggcaacg tggaattttt tatcggcgcg cgccgtggtt gaatatcttg
     3481 aaatcgatca tgtcggtcac cgcggcgacg gcgtcgcctt ggccgccgac ggccaggcaa
     3541 tttacgtgcc ctacgcactc gccggcgaga cggtcgaagt agccgaggtg cacggccatc
     3601 cggaccgcag gcgcctgctc gcggtcaaga ccgcaagcga agcgcgcatc gcgccgttct
     3661 gcccgcattt tggcgtttgc ggcggctgcg cgatccagca ctggcgcgag gacgcctatc
     3721 gcgcctggaa gcgcgacatc gtcatcgaga cgctgaagca ggcaaggctc gactgcgagg
     3781 tgacaccttt gatcgacgcc cacggccttg gccgccgccg cgtcaccctg cacgcgcggc
     3841 tcggcacgca tgaggtcctg aaggtcggct tcgccgcggc gagttcccat gacatcatcc
     3901 cggtcgatca ctgtccgatc ctcgacccgg cgctatcagg cgcattggac gcggcgtggg
     3961 cgctggccga gcccatcctc aaaaacggca agccgctgga tattcagatg acggccacca
     4021 ataatggcct cgatgccgat gtacgcggct cggggccgtt ggcgcctccg gtgattgcga
     4081 cgctgtcggg catcgcggga aaacatcggc tggcgcgact gacgcggcat ggcgaattgg
     4141 tgctgatgcg cgcgtccccg accgtgacca ttggcaccgc cgaggtgacg ctgccgcccg
     4201 gctcgtttct acaggccacg accgcgggcg aagagacgct tgcggcgctg gtcgccgagc
     4261 gttgcggcaa ggcaaggcag atctgcgatc tgttttgcgg ggtcgggccg ttcgcgctgc
     4321 ggctcgcaac cagggcgcgc gtgtccgcct tcgacagcga tgcgggcgcg atctctgcct
     4381 tgcagaaggc cgccactgca acgcgcggtt tgaagccggt gagcgccgag acgcgggacc
     4441 tgttccgccg cccgctggtg ccacaggaac tgcgcgactc cgacgtcgtc attttcgatc
     4501 cgccgcgcca gggcgcacag gcgcaggcgc agcaacttgc ggcaagcaag gtgccggtcg
     4561 tgatcgccgt gtcctgcaat gttgcaacct tcgcccgcga cgcgcgcatc ctgcttgacg
     4621 gcggttacag gatggaaacc gtcacgccgg tcgaccagtt ccgccacacc cctcatgttg
     4681 aactggtggc acggttctca cgctgatgcg gcaccctcgg ttgagcctat ctgccgaact
     4741 cggctcgcgc aggtgggatt cacgtgcgcc gtaatcgttg cgcgagatcg agcaaggaat
     4801 gaaataacga gcgatctcgc tcgcgaacga acgcgaccag accggccccg caactcgcac
     4861 catccctaaa tttttgccgt ttcgctttgt gacggaattg ctacattgcc atgtcagttc
     4921 ggttacgtgc ggagcgaccg atgaggttgg tgttgcaggc gcggtcaaat cccctggcct
     4981 ggtggtgggg actgttggcc ctggtgagca gcgtaaatat cgcattctgg ttcctgctct
     5041 accgcgagtt ctgcaacagt ccggcaggga gttccgccag caactccgac atcggactca
     5101 tgctcctgct ctgcgccggc tacgtgttcg gctgcgcgtt caggtcgttt ttgccgcgcg
     5161 cggatgtgca gcggatttgc ctgttcgaca cctggctctc gagcgttgtt gtcggccggt
     5221 ccgtggcgac cgtggccgag ctctgcttcg cggcacagtg ggccatcatc ctgcgccagc
     5281 tcggcgagat agcgggcgca gagaccacct tgaacgccgc gtggctcgtc gtgccgctga
     5341 tcctcgtggc ggagtgcttc tcatggtacg ccgtactaac caccaactat ctcggcaatg
     5401 ccatcgaaaa ttcgatctgg gccgtagcgt tcctcatcgt cggggtcgcc ctttgccggc
     5461 tgctgcctga attcaatggc gcggtacgct gggcgctcgt catcgcgatc tccgggatcg
     5521 cattctatct ggctttcctc atgaccatcg acgttccgat gtatctgggc cgatggcgag
     5581 cggaccttgc cgatggcagc aggctgctgg gaatcttcga gggcctccgt gacatcagca
     5641 cgcgctggat cgtgacgcat gacttcgcac actggaagga cgagattgcg tggatgtcgc
     5701 tctacttcag cgcggcggtc tgggcgagcc ttgcactctg cgcttgctac tcgctcgagg
     5761 attatctccc gcactatcgc accgaaccga cggcgctgac tgcgcgtgac atgttcggcc
     5821 aaagagtcgc ggcgcaggaa gcggtggctt ttccgttgag gatcgccgag gcgtcgatac
     5881 tgctttcggc gccgttcacg ccgcggcggt gatcttcacg tcgatcaaag ccgcaagctc
     5941 aagttcgacc gggtcccgtt cgccggtcac ggcacaggcc acgattctcc ttgcaagcac
     6001 tgcgcgattg tgaggcgtcc gggccttcgg cggcaaggac tgcaccgccc gatccaggac
     6061 ctgcccaaga agcgaaaggt cttcgggata gaagaccgcg ccttgattgg acgacattga
     6121 cgtcgctccc ccattttcgt tcgcgctgtc ctctaagatt catcgaacgg tttgttcctg
     6181 ttagggcgga aaaaagatca cgaggcgacg cccgttggcc gaagtcgcgg tgggcctttg
     6241 cagccgatag ctaggacgga tacggatatt gcccgcggtg gcagcggcat caaacggcga
     6301 gcactcattg cggggctggc gcgcgcagcg aaacttccgc caggattgat ctcctgcggc
     6361 cccgatctcc tcgtctacac gttgctcaat cacgccgatc aggtgatcga ctgaaatcct
     6421 ccgctactcc acgacgtcga gtttgacttt cgcgatgccc gatccgacca tgcccagcgc
     6481 ctgcgctgcg gaataggaca catccacttc ccgtccgtgc acgtaaggac cccgatcatt
     6541 gacgcgcaca gtcacagagc gtccggtggc ggtattcgtc acgcgcagct ttgttccgaa
     6601 cggcaaggtt ttatgggcgg cggtcaactc gtccggattg aacttttcgc cgctcgcggt
     6661 gcgagtgtcc gagtagaagc tggcaacgcc ctgggaaggc agtttcaagt cgccggtatc
     6721 cttctccgac gcatatggat cgtgccgtct cacgatcgag gtgtgttcga cggctgcggt
     6781 ccgcgtctcg cgatggcgca cgattgccac ctgccgggtg gcaacaaact cggatttcct
     6841 cgatgcaacc gaggactggg cagcgcagcc ggcaagcaat gcggctgcca gagcaatcgc
     6901 aaagcaccga atgacgttca gaccgttagc agccgaaaca ttgctggtgg gcggcatgtt
     6961 atccttgcct cctactcagc cccgccgcaa tccggtcaag caatgcgggc agaacagtgg
     7021 ctggacgcag ccggccacgc cgtgatcgcg cgatttcctt ttcagtgttg ccgtagagct
     7081 acagttttga cggcgcgtgg aacaaatttc acattcgcta ccgtcttgtc gcgtttcgtt
     7141 tcgcagcgaa cgtcagaatc gcgatcatcg cccccatcgg tcctgccaga tcttttcgcc
     7201 gatcaggcag gacacccgcg actccttttc ggcgaccggc acgagccgcc gctcaggcgt
     7261 gcgggaggcg acctcgagca gcagttttgt acgaatcgct tccttgaatt tctcgcgatc
     7321 cttgatcggg accacgaacg acccggggcc gccgatcacg caatcctcgt agtaccaatc
     7381 gaggttgtcg atatccatgg tcgaatagga cggctccttc accatgatcg gcaggccgtt
     7441 gatgacgatg cccttggaca ccgcctcgtc gcgcgcggga gtcacggggg cgccgttgtt
     7501 gttggggccg tcgccggaga tatcgatgac gcggcgtgtg ccgtggtagg gatcctgatc
     7561 gaacagcggc atcgcaaaat agatcgcgcc ggagatcgaa gttcgcgagg cgcggcgtac
     7621 cggcgtcttc atgatctcgc tcgcaactgc atcggccgtt tccggtccgt cgatcagacg
     7681 ccagggaatg atgatcttct ggtcggagga ggcagcccat tcgaaatagg tgatcgaaac
     7741 cttgccgttc ggaaggctct tcagcgcctg caggaattcc ttggagatga tcgcctgcgc
     7801 atagccctcg cgctggatcg cgagttcgtc catgtccatg gaataggaga cgtcgaccgc
     7861 gagcaccaac tcgacgtcga ccgcgttcgc ggcatccttt tccgcctcat gctgctcggc
     7921 tttgtaggtc ggaccgggcg cagcaacgac agcgacgccg cctgcgacaa gcgcgcccgc
     7981 aatcaacaca gccccgatcg acgccaatga gcgcatgagc tgccctccaa acgtgaagga
     8041 tcgtgacacg caaatgaggc cgcgcaaagc acgaacatca tttggccttc acattcgggt
     8101 taaaaatctg cctgccccag cgctgcgcgg cgccgttgca cgcgcgacca tttgggccag
     8161 cgcccgccat ctgctgattg ccgcttggtg tcagggactt ctcgcctgtc cgcgtgccgc
     8221 gatcgcaagg aggacgcgca aaagtgccgg ttccgccgcg ccgcaggagg cgatattctt
     8281 caagctgcag gtttgtgaat ggggcgtgag gaggcgaaaa cttcgatgat cgacgccgtc
     8341 accaaaccca aaaccaaggt ca
//
LOCUS       DATLJT010000638         3695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_956791,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000638 DATLJT010000000
VERSION     DATLJT010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956791"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VM910_14790"
     CDS             <1..229
                     /locus_tag="VM910_14790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVI63833.1"
                     /translation="MGKAVSYSDIANKISAPKASRAVGAAVGKNPVSFVVPCHRALGK
                     DGKLTGYHWGITRKQAILGWEAGQVSASRAA"
     gene            complement(232..855)
                     /locus_tag="VM910_14795"
     CDS             complement(232..855)
                     /locus_tag="VM910_14795"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HVI63834.1"
                     /translation="MSDRLLVLVRHGQSEWNLKNLFTGWKDPDLTEQGIAEAMDAGRK
                     LKAQGLKFDIAFTSVLKRAQHTLDLMLAEIGQTGLATKKDLALNERDYGDLSGLNKDD
                     ARKKWGEEQVHIWRRSYDVSPPGGESLKDTLARTLPYYVQEILPCVLRGERTLVAAHG
                     NSLRALIMVLEKLSPEGILKRELATGVPIIYHLNADATVASKLDLAA"
     gene            complement(883..1698)
                     /gene="dapB"
                     /locus_tag="VM910_14800"
     CDS             complement(883..1698)
                     /gene="dapB"
                     /locus_tag="VM910_14800"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HVI63835.1"
                     /translation="MADMRLIVAGAGGRMGRTLTRVIADTPGAVLAGALEAPGSALLG
                     KDAGVLAGLPANGVTLSADLWALSAEADGILDFTVPGATIANVAIAAQRGLVHVIGTT
                     GLSASDEAVIKSVASRAIVVKSGNMSLGVNLLAALVKRVAASLDESFDIEILEMHHKA
                     KIDAPSGTALMLGEAAAEGRKITLEGHSARGRDGVTGARRSGDIGFASLRGGTAAGDH
                     SVIFAGPSERLTLSHHAEDRAIFAHGALKAALWAHGKPPGFYTMADVLGLSDI"
     gene            complement(1777..2079)
                     /locus_tag="VM910_14805"
     CDS             complement(1777..2079)
                     /locus_tag="VM910_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI63836.1"
                     /translation="MAKGYWIGRVDVHNEEGYKAYAAANPAIFKSFGGRYVVRGGRFT
                     GVEGQSRSRNVVIEFADYDTALACYRLPEYQQNIKVRQPHSIADLIIIEGYDGPQP"
     gene            complement(2099..2812)
                     /gene="pyrF"
                     /locus_tag="VM910_14810"
     CDS             complement(2099..2812)
                     /gene="pyrF"
                     /locus_tag="VM910_14810"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVI63837.1"
                     /translation="MQPSDIAARDRLIVALDLPAVDRAEEVVARLGDAANFYKIGYQL
                     AYAGGLPLVRKLADQGKKVFIDLKLHDIGNTVARGVESIARLGATFLTVHAYPQTMKA
                     AAEARAGTDLKILAVTVLTSYDDDDLHAAGYRLGVSDLVEARAQQAQVLGIDGLVCSP
                     EEAAALRKLVGHQMCLVTPGIRPSGSATGDQKRIMTPARAIAAGADYLVIGRPVLEAA
                     DPKAAAEAIVAEIAPRLVA"
     gene            complement(2820..3398)
                     /locus_tag="VM910_14815"
     CDS             complement(2820..3398)
                     /locus_tag="VM910_14815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVI63838.1"
                     /translation="MAALKILVVPGSLRSGSHNVKLAAAAAYQFVQAGADVTRISLAD
                     FPLPIYDGDLQARSGVPKNAINLKRMIGAHHAVLLVTPEYNSSVPPLVKNTIDWITRV
                     QDGHEIRGQVFREKPFAIAAASESRLGGTRALAALRLILTACHASVIPNQLALSFADQ
                     AYDDMDRLKNSADIEALNALVRQLVEVSQRMM"
     gene            complement(3458..>3695)
                     /locus_tag="VM910_14820"
     CDS             complement(3458..>3695)
                     /locus_tag="VM910_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVI63839.1"
                     /translation="VPAAAMVSGLPLVTKPMLTRLKLIRDAFVALAPGAPFVQFTYSV
                     APPIPKSLPGVSTEASERIWMNLPPARVWVYRKD"
BASE COUNT          669 a         1198 c         1171 g          657 t
ORIGIN      
        1 gatgggcaag gcggtttcct attcggacat tgccaacaag atcagcgcgc cgaaagcgtc
       61 gcgcgcggtg ggagctgcag tcggcaagaa cccggtatcg ttcgtggtgc cctgccatcg
      121 cgcgctcggc aaggacggca agctgaccgg ctatcactgg ggcatcacgc gcaagcaggc
      181 gatcctcggc tgggaagcgg gccaggtgag cgcatcacgc gctgcgtgaa ctcacgctgc
      241 aagatcgagc ttcgacgcca ccgttgcgtc cgcgttcagg tggtaaatga tcggcacgcc
      301 ggtggcgagc tcgcgcttca agatgccctc gggcgacagc ttctccagca ccatgatcag
      361 cgcacgcagg gaattgccgt gcgcggcaac gagcgtgcgc tcgccgcgca gcacgcaggg
      421 gagaatctcc tggacgtaat agggcagcgt gcgcgccagc gtatccttca ggctttcgcc
      481 gccgggcggc gacacgtcgt aggaacgccg ccagatgtga acctgctcct cgccccattt
      541 cttgcgagcg tcgtccttgt tgagaccgga aaggtcgccg tagtctcgct cgttcagtgc
      601 gaggtctttc ttggtcgcaa gcccggtctg accgatctcg gcaagcatca ggtcgagcgt
      661 atgctgcgcg cgcttcagga cagacgtgaa ggcgatgtcg aatttcagcc cctgcgcctt
      721 cagcttgcgg ccggcgtcca ttgcttctgc gatcccctgc tcggtcaggt caggatcctt
      781 ccaaccggtg aagaggttct tcaggttcca ttcgctttgg ccgtgccgca ccagcacaag
      841 aaggcgatcg ctcattgcaa catgtccatt tcggtgaggt ttctagatgt cgctgagccc
      901 cagcacatca gccatggtgt agaagccagg cggcttgcca tgcgcccaca gcgccgcctt
      961 cagcgcaccg tgcgcgaaga tcgcccggtc ttcggcgtgg tgcgacaggg ttagccgctc
     1021 cgacggaccg gcgaagatca cgctgtgatc gccggccgca gtgccgccgc gcaaggaggc
     1081 aaagccgata tcgcccgagc gccgtgcgcc ggtgacacca tcgcggccgc gcgccgaatg
     1141 tccctcgagc gtgatcttgc gtccctcggc cgcagcctcg cccagcatca gcgccgtgcc
     1201 ggaaggcgcg tcgatcttgg ccttgtggtg catttccagg atttcgatgt cgaagctttc
     1261 gtcgagcgaa gccgcgacgc gcttcaccag tgccgcgaga agattgacgc cgaggctcat
     1321 attgccagac ttcaccacga tcgcgcgcga ggcgacgctc ttgatcacgg cctcatcgga
     1381 tgcagacagg ccggtcgtgc cgatgacatg gacaaggccg cgctgggccg cgattgcgac
     1441 attggcaatg gtcgcgcccg gaacggtgaa gtcgagaatg ccatcggcct ctgccgacag
     1501 ggcccacaga tcggccgaaa gcgtcacgcc gttggcaggc aatccggcaa gcacgccggc
     1561 gtctttgccg agaagtgccg aacccggcgc ctccagcgcg cccgcgagca cggctcccgg
     1621 ggtgtccgcg atgacgcgtg tcagcgtgcg acccatccgg ccgcccgcac ccgcaacgat
     1681 caaccgcatg tcggccatgg cttatcctct cacccgccta tagcgggagc gggccattcc
     1741 ggcaaccggt gcggcttaag gatcagccct cgaggatcag ggctgcgggc cgtcatagcc
     1801 ctcgatgata atcagatcgg cgatggaatg cggctgccgg accttgatgt tttgctggta
     1861 ttccggcaag cgatagcagg cgagtgccgt gtcatagtcc gcaaattcga tcacgacgtt
     1921 gcgcgaacgg ctctggcctt ccacgcccgt gaacctgccg ccgcgcacga cgtagcgccc
     1981 gccgaaactc ttgaagattg cgggattcgc agcggcgtag gctttgtagc cctcctcgtt
     2041 gtgaacgtcg acgcgaccga tccagtagcc ttttgccatg tgttaagccc ttctctcctc
     2101 acgcaacgag ccgcggcgcg atctcggcca cgattgcctc ggcagcggct ttgggatcgg
     2161 cagcttccaa caccggacga ccgatcacaa gataatcggc gcccgccgcg attgctcgcg
     2221 ccggcgtcat gatccgcttc tggtcgccgg tcgccgagcc tgacgggcga atacccggag
     2281 tcaccaggca catctggtgg cccacgagct tgcgaagtgc agcagcttct tccggcgagc
     2341 aaaccaggcc atcgatccca agcacctgcg cctgctgggc gcgggcttcg accagatcgg
     2401 aaacgccgag ccgatagccg gcggcatgca gatcgtcatc gtcataggag gtcagcacgg
     2461 tgacggcgag aattttcagg tccgttcccg cgcgcgcctc ggccgccgcc ttcatggtct
     2521 gcggataggc gtgcacagtg aggaaggtcg cgcctagtct ggcgatgctt tcgacccccc
     2581 gcgcgacggt gttgccgata tcgtgcagct tcagatcgat gaagaccttc ttgccctgat
     2641 cggcaagctt gcgcacgagc ggcaatccgc cggcataggc caactgatag ccgatcttgt
     2701 agaagttcgc ggcatcgcca aggcgcgcca ccacctcctc tgcgcggtcc acagcgggaa
     2761 ggtcgagcgc tacgatcaac cggtcgcggg cagcaatatc agacggctgc atggtcacct
     2821 cacatcatgc gttgcgagac ttcgacgagt tgccgcacca gcgcattcag cgcctcgata
     2881 tcggccgaat tcttcaatct gtccatatcg tcataggcct ggtcggcgaa agacagcgcg
     2941 agctggttgg gaatgacgct cgcatggcag gcggtcagga tcagccgcag cgccgccagc
     3001 gcgcgcgttc cgccaagccg gctctcggat gccgccgcga tggcaaaggg cttttcgcga
     3061 aacacctggc cgcggatttc gtggccatcc tgcacgcgcg tgatccagtc gatggtgttc
     3121 ttcaccagcg gcgggacaga cgaattgtat tccggggtga ccagcaggac ggcgtgatgc
     3181 gcaccgatca tacgcttgag attgatcgcg ttctttggca cgccggacct ggcctggagg
     3241 tcgccgtcat agatcggtag aggaaagtcg gcgagcgaaa tgcgggttac gtcagcgcca
     3301 gcctggacga attgataggc cgcggccgcc gcgagcttca cattgtgcga gccgctgcgc
     3361 agcgagccgg gaaccaccag gattttcagc gcggccataa gttattttgc gttcgggacc
     3421 atcccgccac gctcctccag ccgcggcagg cgggtcttca gtccttccga tacacccaga
     3481 cgcgcgccgg cggaaggttc atccagatcc gctcggacgc ctcggtggac acgcccggca
     3541 acgatttcgg gattggcggc gccaccgaat aggtgaactg aacgaagggc gcgccgggcg
     3601 cgagcgcgac gaaggcatcg cggatcagct tcaggcgcgt cagcatcggc ttggtcacca
     3661 gcggcagccc cgacaccatc gccgcagcag gcacg
//
LOCUS       DATLJT010000639         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2566604,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000639 DATLJT010000000
VERSION     DATLJT010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566604"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VM910_14825"
     CDS             complement(<1..647)
                     /locus_tag="VM910_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI63840.1"
                     /translation="MLAQSLPVIPAAPKVHAKDLPIWRHLLTAQRNSIAIFPDYVFDN
                     LFGRRTLLGVDAVLINDPEGIGHVFATSAANYVRPTMMPRVLRPLLGQGIFLAEGTEW
                     RRQRRVLSPAFTPHNIGLLLPHFTAAANDLLRRLERGPIADLSAAFQIATLEAALRTL
                     FSMPDSIQRARLAAMVRGYVEGPGHPNILDMLARDESAYAFATRKRRTFQKAWFAA"
     gene            1328..3394
                     /locus_tag="VM910_14830"
     CDS             1328..3394
                     /locus_tag="VM910_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVI63841.1"
                     /translation="MPGLDSRPTLEAPDDDPYLWLEEIESARVLDWVEAQNAATLQRF
                     NDAGVATDHKVLKTIFDRPDNIPFPNRRAGRLFNPWRDAANPRGVWRTTTLESFRRDT
                     PDWDVLIDVDALATKEGEDWVWRGGVSLPPRHDRAIVQLSRGGSDAVALREFDLSTRD
                     FVPDGFSLPESKSSAVWLDRDTLLLSSPLGSGMATRSGHARTVRLWRRGSDPLLAPVV
                     FETSEKRTGVWTIVDREVPEERVWFVESLGIVDANVWIGDRSGPKTRVDIPSDCWAQV
                     GRGWLAIKPRTSWNVGGETYETDTVIGMPFSAFLAGERRFTRLFKPANRRALQGFFWC
                     GGRLVLSILDDLRPVFEVITPSDGEWSREVITGLPDLGTAHVWPFDPYPEESNGDLLA
                     AAQDQLTPPSLYLIEPRAAPQLLKRAPQAFDPTGLVGTRHEAVSSDGTRIPYVQVGPA
                     GETGDAPVHLYGYGGFGISMPPYYNSAIGKLWLERGGTSVIAHLRGGREFGTAWHEAA
                     RREGRRLAHDDFAAVAADLVRRGVTRPGRIAAEGGSNGGLLIANMLTRYPERFGALFC
                     TMPVIDMRRYAKLLIGPAITDEYGDPDKPEDWSFLAEISAYHVAAPGHTYPPILIATS
                     RRDDRAHPGHARKMAAKLQAMNYEAHFYELATGGHSYGKDHDDMAAFISLGYAFLRRA
                     IGWQLD"
BASE COUNT          653 a         1078 c         1088 g          645 t
ORIGIN      
        1 gcagcgaacc acgccttctg aaacgtgcga cgcttgcgag tcgcaaatgc gtaagctgat
       61 tcatcgcgtg ccagcatgtc caaaatgtta ggatgccctg gtccttcgac gtagcctcgg
      121 accatggcgg cgagcctcgc ccgctggata ctgtcgggca tcgagaagag cgtccgcagt
      181 gcagcctcga gcgtcgctat ctggaaagcc gctgaaagat cggcaatcgg gccgcgctcc
      241 aggcgccgta agagatcatt ggcagcggcg gtgaaatgcg ggagcagaag gccgatgttg
      301 tgtggtgtga acgccggcga caacacgcgt cgctgtcggc gccactcggt accctcggcg
      361 agaaaaatcc cctggcccag caacgggcgg agaactcgcg gcatcatcgt cgggcggaca
      421 taattggctg cgctcgttgc aaacacatgc ccgatacctt ccggatcgtt gatgaggacg
      481 gcgtctacgc cgagaagcgt tctgcggcca aatagattgt cgaagacgta gtccgggaag
      541 atcgcgatgc tattgcgttg cgctgtcagc aggtgacgcc aaatcggtag gtccttggca
      601 tggaccttcg gtgctgcggg aatgaccggt agggattgcg caagcatacg ggcctcgcac
      661 tcgagaacgc gtacgggctc gcgccaaagc ccctaaggca aatcgtcgac accaacgaaa
      721 tcgttccggt gctaccgcgc gttcactgtg cgatattgat aggggtatgt ggcgattggt
      781 tggctccgtc cgaagaacaa tcgatagccg actcgagcag aaaccgcgtc ggtgcaacct
      841 cggtggtgca ccctattggc tcatcacgtc agcccgcgtg ctggccacac acagccaacg
      901 tcgaagcatg accggactac ccgctgaagc gaccagatgc cctctcccga ggcgtagcga
      961 gaaatcgcaa gccgtcgcag acttgccgtt tggagtggcg ggcagcgaca tcataggggc
     1021 acaacgaagc aatagtgccc atcttcatcg accgcggcgc gccacctacg gttttatatg
     1081 agattattgc tgggacgggc gcgtgtgatg cctctagccg ccgattgccg cacacggaac
     1141 ctgctgccaa tcgcctggac gtagtgcgag agtatttcgg tactcgtctt ggccgagctg
     1201 cccgtcgcat ctgcttgctt tggacctcaa gcgtatcgaa ggttttgcgc ttcgacccgc
     1261 cctgttgtcg ttccgtcgga agatgccgat atagactcct tccttctacc aaccagggag
     1321 ctttatcatg cctgggctcg attcgcgacc gacgctcgaa gcacctgacg acgaccccta
     1381 cctctggctc gaagagatcg agagcgcgcg cgtgcttgac tgggtcgagg cacagaatgc
     1441 ggcgacgttg caacgtttca acgatgcagg cgtggcaact gaccacaaag ttctcaagac
     1501 catctttgac cgcccggaca atattccgtt cccgaaccgt cgtgccggga ggctgttcaa
     1561 tccctggaga gatgccgcca atccccgggg cgtttggcgc acgacgacgc tcgagagctt
     1621 tcgccgcgat acgcccgact gggacgtgct catcgatgtc gatgcgctcg caaccaagga
     1681 aggcgaggat tgggtttggc gcggcggcgt gtcgctgccg ccaagacatg atcgcgccat
     1741 cgtgcaatta tcgcgcgggg gctcagacgc cgtcgcgctg cgcgaattcg acctatcgac
     1801 acgtgatttc gtgcccgacg ggttcagctt gcctgagtcg aagagcagcg ctgtctggct
     1861 cgaccgcgac acgctgcttc tatcgtctcc gctcggatcc ggcatggcga cccgcagcgg
     1921 ccatgcgcgc acggtgcggc tgtggcggcg cgggagcgat ccgctgttgg ccccggtcgt
     1981 cttcgagacg agcgaaaaac gtacgggcgt ctggaccatc gtcgaccgag aggtccccga
     2041 ggagcgcgtc tggttcgttg aaagccttgg catagtcgac gcgaatgtct ggatcggcga
     2101 ccgcagcggg cccaagaccc gcgtcgatat tccgtccgat tgctgggcac aggtgggtcg
     2161 cggctggctc gcaatcaaac cccgtacttc ctggaacgtc ggcggcgaaa cctacgaaac
     2221 cgacacggtc atcggcatgc ctttttcggc ctttctggcc ggcgagcgtc gcttcacgag
     2281 attgttcaag ccagccaatc ggcgcgcgct gcagggattc ttctggtgcg gcggccggtt
     2341 ggtgctatcg atcctcgacg atctgcggcc ggtgttcgag gtgatcaccc cgtccgacgg
     2401 cgaatggtcg cgagaggtca tcaccggcct cccggatctt ggcaccgccc atgtctggcc
     2461 gttcgatccc tatcccgagg aatcgaacgg tgatctcctt gcagccgccc aagatcagct
     2521 caccccgcca tcgctctatc tcatcgagcc tcgcgccgca ccacaattgc tgaagcgcgc
     2581 accgcaggct ttcgacccga ccggccttgt cggcacgcgg cacgaagccg tgtcgtcgga
     2641 cggaacgcgc atcccgtatg tgcaggtggg accggccggt gagacaggcg acgcgccggt
     2701 ccatctctat ggctacggcg gcttcggaat atcgatgccg ccctactaca actcagccat
     2761 cggcaaactc tggctcgaac gtggtggcac cagcgtgatt gcacacctgc gcggcgggcg
     2821 cgagttcggc acggcctggc acgaagcggc gcgccgcgag ggcaggcggc tcgcgcacga
     2881 cgatttcgcg gccgtcgccg ccgaccttgt ccggcgaggg gtgacccggc ctggccgtat
     2941 cgcggcggaa ggcgggtcga atgggggcct tctcatcgcc aacatgctga cgcggtatcc
     3001 ggagcggttc ggcgcgctgt tctgcacgat gccggtcatc gacatgcgtc gctacgccaa
     3061 gcttctcatc ggtccggcga ttaccgacga atacggcgat cccgataagc cggaggattg
     3121 gagcttcctc gcggagattt ccgcgtatca cgtcgcggcg ccggggcata cctacccacc
     3181 gatcctgatc gcgacaagcc gccgcgatga ccgagcgcat ccgggccacg cccgcaagat
     3241 ggctgccaag ctgcaggcaa tgaactacga agcacatttc tacgagcttg cgaccggagg
     3301 gcacagttac ggcaaggacc atgacgacat ggcggcgttc atatcgctcg gctacgcgtt
     3361 cttgcggcgc gccatcggat ggcagctgga ttgaatgcca tgcagcctgc gttttctgct
     3421 gagtctatcg gcaggaaaaa caaagagccc ggcgtcgcgg ccgg
//
LOCUS       DATLJT010000640         3649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3944029,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000640 DATLJT010000000
VERSION     DATLJT010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944029"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VM910_14835"
     CDS             <1..112
                     /locus_tag="VM910_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HVI63842.1"
                     /translation="TSPLARALIGKKKGSSVEVMAPGGAKAYEITKVEWR"
     gene            254..670
                     /locus_tag="VM910_14840"
     CDS             254..670
                     /locus_tag="VM910_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI63843.1"
                     /translation="MNADRLQPYVLSLFRFVTGLLFFHYGVAKLFKFPAVEMFEEVTP
                     LSLWGVAGMIEFVLGGMLMLGLLTRLVAFILSGEMAFAYFIEHFPHSFYPLLNEGALA
                     IMFCFGCLYLATAGGGPISLDAALGLKIPQLGPSRS"
     gene            complement(673..1128)
                     /locus_tag="VM910_14845"
     CDS             complement(673..1128)
                     /locus_tag="VM910_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI63844.1"
                     /translation="MADHGETRLIFPALAGLYESCAPFSYALMRFATGAILVPHGVVK
                     ILNTPIAKFAPNIAAKGLPFPEALAYLTYFAESVAAVCLAIGLFTRVAAAIIGIEMIV
                     IVILFQWQFGYFWTNRGYEYALLWALLCIAIFFKGGGRYSVDRLIGREF"
     gene            1361..1819
                     /locus_tag="VM910_14850"
     CDS             1361..1819
                     /locus_tag="VM910_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI63845.1"
                     /translation="MTNWDRLAATYQPYVLSLFRFITGLLLVQYGVAKILKFPALPYF
                     ANPPPLTMTAGAIELVLGALLMIGLFTRPAAFILSGEMAFAYFIGHMFKTGAPVFHPL
                     NNGGTAAILFCFACLYLSASGGGPVSVDATLKKRGRFDHSHDGRFGQATG"
     gene            complement(1826..2305)
                     /locus_tag="VM910_14855"
     CDS             complement(1826..2305)
                     /locus_tag="VM910_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI63846.1"
                     /translation="MSKNLDEIDLKILSEIQADGRITNVELAKRVGISPPPCLRRVRT
                     LEEEGYIKGYRGLLDPRRLGFDVTVFASVHLSSQAEADLRAFENFVRAEPLVRECWML
                     SGEVDFILKCVAPDMATFQEFVTHLTAAPHVRNVRTSLVLHNSKYEAAVPLEVKPHD"
     gene            2591..3556
                     /gene="trxB"
                     /locus_tag="VM910_14860"
     CDS             2591..3556
                     /gene="trxB"
                     /locus_tag="VM910_14860"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HVI63847.1"
                     /translation="MSVPVHAKVVIIGSGPAGYTAAIYAARAMLEPILIQGIQPGGQL
                     TITTDVENYPGFADVIQGPWLMEQMEKQAAHVGTKIVTDFVTKLELSQRPFRLTCDSG
                     DVYFAETVVLATGAQARWLGLPSEEKFKGFGVSACATCDGFFYRGKEVVVVGGGNTAV
                     EEALFLTNFASKVTIVHRRDHFRAERILQDRLFKNSKIKVIWNSVVDQIDGAETPAKV
                     TRVRLKNVKTGALTELPADGVFVAIGHAPATDLVKGQVKLKPSGYVEVAPNSTATSVP
                     GLFAAGDVADEVYRQAVTAAGLGCMAALEAERFLALRASERAAAE"
BASE COUNT          684 a         1116 c         1093 g          756 t
ORIGIN      
        1 cacctcgccg ctcgcgcgcg cattgatcgg caagaagaag ggctcctccg tcgaggtcat
       61 ggcgcccggc ggcgccaagg cctacgagat caccaaggtc gagtggcgct agcagccgat
      121 ttgaaattcg tcgctttgcg agggccgcgc tttgcgcggc cttctctttg gccgacatgc
      181 cgtatcattg tcggggccgc cgtgatattg caagtcgcca acatccggcg ggggcatcgc
      241 gcaggaggaa acgatgaacg ctgatcgtct tcagccttac gtgttgagcc tgttcagatt
      301 cgtgacgggt ctcctgttct tccactatgg cgtcgcgaaa ttgttcaaat ttcccgccgt
      361 cgaaatgttc gaggaggtca cgccgctgtc gctgtggggc gttgccggca tgatcgagtt
      421 cgtgctcggc ggcatgctga tgctcggtct gctgacacga ctcgtcgcct ttatcctgtc
      481 gggcgagatg gcgttcgcct atttcatcga gcatttcccg cacagtttct acccgctgct
      541 caacgaaggc gcactcgcca tcatgttctg tttcggctgc ctttacctcg ccacagccgg
      601 cggtgggccg atcagccttg atgcggccct tgggctgaaa atcccgcagt tgggcccgtc
      661 gcgcagctag gctcagaact cccggccgat caaccgatcg accgaataac ggccgccgcc
      721 cttgaagaag atggcgatac aaagaagcgc ccagagcagt gcgtattcgt agccgcgatt
      781 ggtccagaaa tacccgaatt gccactggaa caggatgacg atcacgatca tctcgattcc
      841 gattatggcg gcggctactc gcgtgaacag accgatcgca aggcaaactg ccgcgaccga
      901 ctcggcaaaa taggtcaggt aggcaagcgc ctcgggaaac ggcagccctt tggctgcgat
      961 attgggcgcg aacttcgcaa tcggcgtgtt gaggatcttg accacgccgt gcggcaccag
     1021 gatcgcgccg gtggcaaagc gcatgagtgc gtaagagaac ggcgcgcagc tctcgtagag
     1081 acctgcgagc gccggaaaga tcagcctggt ttctccgtga tcggccatcg catccccccg
     1141 gttcgtcgct ccctgcgcag tagaacctgc caatagtcgc acaacacgca actgcgaaac
     1201 accgaccaag catccctatt ccgccgggcg catagcggag ccaatgcaat tcacaattgc
     1261 gggagcatac gagccaagcg ttcgggcctg cgaaccaatt gtcgcctcaa tcgttgagcg
     1321 tttagccacg gggcgcgttt gggtggaaag agatagagat atgacgaact gggatcgcct
     1381 tgccgcgacc tatcagcctt acgtgttgag cctgttcagg ttcatcactg gactgctgtt
     1441 ggttcaatac ggcgttgcaa aaatcttgaa gtttccggca ctgccctatt tcgccaatcc
     1501 gccgccgctg accatgaccg ccggcgcgat cgagctcgtg cttggcgcgc ttctgatgat
     1561 cggcctgttt acgcggccgg ccgcgttcat cctgtcgggc gaaatggcgt tcgcctattt
     1621 catcggccat atgttcaaga ccggcgcgcc tgtgtttcat ccgctcaaca acggcggcac
     1681 ggcggcgatc ctgttttgct tcgcctgcct ctatctgtcc gcgtcaggcg gcgggccggt
     1741 cagcgtcgac gccacgctga agaagcgggg cagatttgac cattcgcacg acggccgctt
     1801 cggacaggct accgggtgag cgcgctcaat cgtgcggctt cacctcgagc ggcactgcgg
     1861 cctcgtattt ggagttatgc agcaccagtg aggtcctgac gttgcgcaca tgcggtgcgg
     1921 cagtcaggtg cgtcacgaat tcctgaaacg ttgccatgtc gggtgcgacg cacttcagga
     1981 tgaagtcgac ctcgcccgac agcatccagc attctcgcac caacggctcg gcgcgcacga
     2041 aattctcgaa cgcgcgcagg tccgcctccg cctgactcga cagatggacc gaagcaaata
     2101 ccgtgacgtc gaagccgagc cttcgcgggt ccagcagccc gcggtagccc ttgatatagc
     2161 cttcctcctc cagggtgcgg acccgccgca ggcagggcgg cggtgaaatt ccgacgcgct
     2221 tggccagttc gacattggtg attcggccat cggcctggat ttcgctcagg attttgaggt
     2281 caatctcgtc aagattcttg gacacgtgaa ttccggcttc tctggctgac atgcgggggc
     2341 gctatcccga ggaactctct tagcgcagct cccttggatt gcgcaatttt attgcgcgtg
     2401 cggcgcgtca atttccttcg tcacgggaaa gtttctgagc ggccattgga aaattcgccg
     2461 atatggattg caattcttgc atggctcgcc agatatccta gacttggatt gatccctttc
     2521 ggcgctgtcg cgatgcctgt cccgcgcatt tcgctcacgc gctgggccct gcaaccgaga
     2581 cattcgcctc atgtccgttc ctgtgcacgc caaagtagtc atcatcggtt ccggtccggc
     2641 cgggtataca gccgcgatct acgccgcgcg ggcgatgctg gagccgattc tgattcaggg
     2701 gattcagccg ggcggccagc ttaccatcac caccgacgtg gaaaactatc cgggctttgc
     2761 cgacgtcatc cagggaccct ggctgatgga acaaatggaa aagcaggcgg cccatgtcgg
     2821 caccaagatc gtcaccgatt tcgtcaccaa gctcgaactg tcgcagcggc cgtttcggct
     2881 gacctgcgac tcgggcgacg tctatttcgc cgagaccgtc gtcctggcca ccggcgcaca
     2941 ggcgcgctgg cttgggcttc catcggaaga gaaattcaag ggctttggcg tctcggcctg
     3001 cgcgacatgc gacggatttt tctatcgcgg caaggaggtc gtcgtcgtcg gcggcggcaa
     3061 tacggcagtc gaggaggcac tgttcctcac caatttcgcc tcgaaagtca ccatcgtgca
     3121 ccggcgcgat cacttccgcg ccgaacgaat cctgcaggat cgcctgttca agaattcgaa
     3181 gatcaaggtg atctggaact ccgtcgtcga ccagatcgac ggcgccgaga ccccggccaa
     3241 ggtcactcgt gtccggttga agaacgtcaa gaccggcgcg ctgacggagc ttcccgccga
     3301 cggcgttttc gtcgcgatcg gtcacgcgcc ggcgaccgac ctcgtcaaag gccaggtgaa
     3361 gctgaaaccg tcgggctatg tcgaggttgc gccgaattct acagcgacct cggtgcccgg
     3421 cctgttcgcc gccggcgacg tcgccgacga ggtctatcgc caggcagtca ccgccgctgg
     3481 gcttggttgc atggcggcac tggaggctga acgttttctc gcgctgcgcg ccagcgaacg
     3541 cgcggcggct gaatgacttt gatccatggc taaaactcgc gacggatttt ccgatatgga
     3601 ttgggacaag ctgaaggtgt ttcacgcagc ggcggaagca ggcagcttc
//
LOCUS       DATLJT010000641         6269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_498516,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000641 DATLJT010000000
VERSION     DATLJT010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498516"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3019
                     /gene="addB"
                     /locus_tag="VM910_14865"
     CDS             <1..3019
                     /gene="addB"
                     /locus_tag="VM910_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HVI63848.1"
                     /translation="YLPTRRAVRVAREVFLEELKTDAAVLPRITPLGDIDEDELAFAE
                     EAEQFAGAAAMDIPPRLGELDRRLTLAKLVAAWSKGEVLAPLVVGGPASTLALASDLA
                     RLMDDMVTRGVAWEALDGLVPDQLDQYWQYSLEFLRIARKLWPDHLAEIGKIEPAARR
                     DLLIEAEAARLSVHHKGPVIAAGSTGSMPATAKFLHAVAQLKQGAVVLPGLDVDLDED
                     AWQAIAGERDAHGKFTAPPASNHPQFAMHALLQRFGLVRNDVEILVPPAPHGRDTLLS
                     EAMRPSNATAEWHARLKQPGTATKISAGMDQLAVIEAPNAEMEALAIAVAMREARHLD
                     KTAALVTPDRALARRVMAALERWNLDFDDSGGDALIETPAGTFARLAAEAVAKGLEPP
                     TLLALLKHPLLRLGRNGNAFSGAISSLELALLRGTRPQAGTAGLAREFARFREELDKL
                     HRGEISSLHRSEPRARLKGHELDRVQSLIVALQKALAPFEGLAALKPHDFAELAARHR
                     EILIALSRDESGVAVAFEGQDGLALAAALDDLLAESKLSGLPVALADYPDVFQTAFAD
                     RAVRRVEKPGARLHIYGPLESRLMRTDRMIVGGLIEGIWPPTPRIDPWLSRPMRHELG
                     LDLPERRIGLSAHDFAQLLGTPDVILSHAAKAGGAPAVASRFLHRLEAVAGEARWNDA
                     KLAGERYVRFAQELDRPDTVKPITQPAPKPPRATRPLKLSVTAIEDWLRDPYTIYAKY
                     ILRLDALDPVDMPLSAADRGSAIHDAIGDFTKAFAASLPDDPARVLKDIGQKYFAPLM
                     ERPEARALWWPRFQRIANWFAEWEIARRGNIDAIEAEIRGEIPLHLGNERIFFLSARA
                     DRIERRTDGTFAILDYKTGQPPSGKQVRMGLSPQLTLEAAILREGGLKGIPARASVGE
                     LAYVRLSGNNPPGEHRALELKIRQGDTAQHPDAAADYARQQLEALITKFENEDEPYRS
                     LNLPMWTNRYGTYDDLARIKEWSAAGGLGIEEW"
     gene            3013..>6269
                     /gene="addA"
                     /locus_tag="VM910_14870"
     CDS             3013..>6269
                     /gene="addA"
                     /locus_tag="VM910_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HVI63849.1"
                     /translation="MVKALRSIPEAVRATQARASDPASSSFVSANAGSGKTHVLVQRV
                     IRLLLSDVPPEKILCITFTKAAAANMAERVFSTLGHWVTLDDASLDAAIREAGIAHPN
                     AKIRNKARKLFASALETPGGLKVQTIHALCTRLLQQFPFEANVPARFAVLDGRDQADM
                     MERANLKVFLEASRDPESAVGRALLTAMSGAADVTFKDVVREACLSRDHFLAWVDTAG
                     SAETATAEMAAALGVTQDDRIEDVDRKILDGPYLPRRRWAEIVDVLDAGNKSEREHAA
                     RLRAASQTSGADQVDTYLGVFFTDDKTPRKAVLTKRFCDDNPSLARLFDAECARLPAL
                     IEKRRALVVRDRTEALLHIATAAAANYRREKEERGLLDYDDLIDKTLSMLRRVSPGWV
                     HYKLDRGVDHVLIDEAQDTSPGQWDIIDHIISEFTAGSGARDGVTRTIFAVGDEKQSI
                     FSFQGAAPREFDQRRRTLQRKFTDAGLTFDPVSFTYSFRSGPAILQSVDHVFRDEAIY
                     RSIHSVDIGYPIHHSLADAGPSLIDFWELAQADDRNDIEGWRAPFDGVSITSGEVKLA
                     RRIQAEIKRLIDGGTMTGPAGNRRRLSHGDVLVLVRRRGNVFDAVIQALKHAGVPVAG
                     ADRLKLTEHIAIIDLMNLADALLLPQDDLALAVALKSPLFGLTDDDLFALAWERKTSL
                     RASLAQQAATNEKFRNVENRLAQCERYFAHMTPFAFYAWLLGGDGGRARILKRLGHEA
                     NDALDEFLELALNYERKAPASLQGFVAWLRAADTEVKRDMEISRDEVRVMTVHGAKGL
                     EASVVFLVDTTSSPSDTQRLRLIQLPRGNDGEVVVWAGKMADDPKVVANARRAMLEET
                     EDEYRRLLYVAMTRAADRLIVAGCKPGNRNNVRKLCWYDLIETGLTRSGLNEQIIDTA
                     EGPVKRFAHSEDVAANAGAAAATVGAVRSELPAWLRAPLGPQASNAALLRPSDAGAGS
                     HGIRTSETLFERARALQRGTLVHRLLQSLPDIAVARRRDSAIRFLERNAAAWSAGERE
                     ELAQKVLGLIADPRFAPVFGEGSRAEVAIVGRLALPGRPEALVSGQIDRLVVTDN"
BASE COUNT         1098 a         2031 c         2079 g         1061 t
ORIGIN      
        1 ctatctgccg acgcggcgcg cggttcgcgt cgctcgcgag gtgttcctcg aggaattgaa
       61 gacggatgca gccgtgttgc cgcgcatcac gccgctcggc gacatcgacg aggacgaatt
      121 ggcctttgcc gaggaggccg agcaatttgc cggcgcggcg gcgatggaca ttcctcccag
      181 gcttggcgaa ctcgaccgcc gactgacgct cgccaagctg gtcgcggcct ggtccaaggg
      241 cgaagtgctg gcgccgctcg tggtcggagg tcccgcatcg acgcttgcgc tcgcttccga
      301 ccttgcgcgt ctgatggacg acatggtgac acgcggcgtg gcgtgggagg cgctcgacgg
      361 gctggtgccc gatcagctcg accaatactg gcagtattca cttgagttcc tgcgcatcgc
      421 acgaaagctc tggccagatc atctcgccga gatcggcaag atcgaaccgg cggcgcgacg
      481 cgatctgttg atcgaagcgg aggccgcgcg gttgagcgtg catcacaagg ggccggtcat
      541 tgccgcgggt tcgaccggtt cgatgccggc aacggcgaaa ttcctccacg ccgtggcgca
      601 gctcaagcag ggcgcggtgg tattgccggg gctcgatgtc gacctcgacg aagacgcctg
      661 gcaggcgatc gccggggaga gggatgcaca cggcaagttc accgcgcctc ccgcctccaa
      721 ccatccgcaa tttgcgatgc atgcgctgtt gcaacgcttc ggccttgtgc gaaacgacgt
      781 cgagattctc gtcccgcctg cgccgcacgg acgtgacacc ctgttgtcgg aagccatgcg
      841 cccctccaat gccacggcgg aatggcacgc gcggctcaag cagcccggga ccgccacgaa
      901 gatttcggca ggcatggacc aactcgccgt gatcgaggcg ccaaatgcgg aaatggaagc
      961 gctcgccatt gccgtcgcca tgcgcgaggc gcggcatctg gacaaaacgg cggcactggt
     1021 cacgccagac cgagcgttgg cgcgccgcgt gatggccgcg ctcgagcgct ggaatctcga
     1081 tttcgacgat tccggcggcg atgccctgat cgagacgccg gccgggactt ttgcccggct
     1141 tgcggcggaa gccgtcgcca aggggctgga gccgccgacc ttgctggcgc tgctgaaaca
     1201 tccgctgctc cggctcggcc gcaacgggaa tgccttcagt ggcgccatct cctccctcga
     1261 gcttgcgctg ttgcgcggga cccgtccgca agcgggcacg gcaggcctcg cccgcgaatt
     1321 cgcgcgcttc cgcgaagagc tcgacaaatt gcatcgcggc gagatttcat cgctgcatcg
     1381 atccgagccg cgggctcgcc tgaagggtca tgaactcgat cgcgtacagt cgttgatcgt
     1441 cgccttgcag aaggcgctcg cgccgttcga gggtctcgcc gcgctcaagc cgcatgattt
     1501 tgccgagctt gcggcgcggc accgcgaaat tctgatcgcc ctgtcgcgcg acgagagtgg
     1561 tgtggccgtc gccttcgagg ggcaggacgg gctggcgctc gcagcggcgc tcgacgatct
     1621 gcttgcggag agcaagctca gcggtctgcc ggttgcgttg gcggattatc ccgacgtgtt
     1681 tcagacggcc ttcgccgatc gcgcggtacg gcgggtagaa aagccgggcg cgcggctgca
     1741 catctatggc cctctggaat cgcggctgat gcggaccgac cgcatgatcg tcggcggttt
     1801 gatcgaaggg atatggccgc cgacgccgcg catcgatccc tggctgagcc gcccgatgcg
     1861 gcatgagctc gggctcgatc tgccggagcg gcgcatcggc ttgtcggcgc acgattttgc
     1921 gcagctcctc gggacccccg atgtgatcct cagccatgcg gccaaagccg gcggcgctcc
     1981 ggcagtcgcc tcgcgattcc tgcatcggct tgaggccgtc gccggcgaag cgcgctggaa
     2041 cgatgcgaag cttgcgggcg agcgctatgt gcgtttcgcg caagagctcg atcgccccga
     2101 tacggtcaag ccgatcacac aacccgcgcc aaaaccaccg cgcgccacgc gcccgctcaa
     2161 gctttccgtc accgcgatcg aggactggct gcgcgatccc tacacgatct acgccaaata
     2221 tatcctcagg ttggacgcgc tcgatcccgt cgacatgccg ctgtcggcgg cggaccgggg
     2281 atcggcgatc catgacgcaa tcggcgattt caccaaggca ttcgcggcaa gcctgcccga
     2341 cgatcccgcg cgcgtcctga aggacatcgg ccaaaaatat ttcgcgcctc tgatggagcg
     2401 gcccgaggcg cgcgcgctgt ggtggccacg cttccagcgt atcgccaact ggttcgccga
     2461 atgggagatc gctcgacgtg gcaatatcga cgcgatcgag gcggaaatca gaggcgaaat
     2521 tccgcttcat ctcggcaacg agcgcatctt ttttctctct gcgcgcgccg accgtatcga
     2581 gcgccgcact gacggcacgt tcgcgatcct cgactacaag accggtcagc cgccatcggg
     2641 caagcaggtg cggatggggc tgtcgccgca gctcacgctg gaggctgcga tcctgcgcga
     2701 aggcggcttg aagggcattc cggcgcgcgc atcggtcggc gaactcgctt atgtcaggct
     2761 gagcggcaac aatccgccgg gagagcacag ggcgctggaa ctgaagatca ggcagggcga
     2821 caccgcgcag catcccgatg ccgccgccga ttatgcacgc cagcagctcg aagcgctgat
     2881 cacgaaattc gagaacgagg atgagcctta tcggtcgctg aacctgccga tgtggaccaa
     2941 tcgctacggc acatatgatg acctcgcacg gatcaaggaa tggtccgcag ccggcgggtt
     3001 ggggatcgag gaatggtgaa ggctctgcgt tcgatcccgg aggcggtccg cgcgacgcag
     3061 gctcgcgcgt ccgatccggc ctcatcgtcc ttcgtctcgg cgaatgccgg ttccggcaag
     3121 acgcatgtgc tggtccagcg cgtcatcagg cttctgctct ccgacgtgcc gccggaaaag
     3181 atcctctgca tcaccttcac caaggcggct gctgccaaca tggcggaacg ggtgttttcc
     3241 acgctcggcc attgggtgac gctcgacgac gcgtccctcg atgccgcgat ccgcgaagcc
     3301 ggcatcgcgc atccaaacgc gaagatacgg aacaaggcgc gcaaattgtt tgccagcgcg
     3361 ctggagacgc cgggcggcct gaaggtgcag accattcacg cgctgtgcac ccgcctgctg
     3421 cagcagtttc cgttcgaggc gaacgtgccg gcgcgctttg ccgtgctcga tgggcgcgac
     3481 caggccgaca tgatggagcg tgccaatctc aaggtgtttc tggaggcctc gcgcgatccg
     3541 gaaagcgccg tcggccgcgc gcttctgacc gcgatgtccg gcgccgccga cgtcaccttc
     3601 aaggatgtcg tgcgcgaagc ctgcctgtct cgcgaccact tcctggcctg ggttgatact
     3661 gcaggcagcg ccgagacggc cacggccgag atggcggcgg cgctcggcgt cacccaggac
     3721 gatcgcatcg aggatgtcga tcgcaagatt ctggacgggc catatctgcc gcggcggcgc
     3781 tgggccgaga ttgtcgacgt gctcgacgcc ggcaacaagt ccgagcgcga gcacgcggca
     3841 cggctgcgcg cggcgtcgca aacctctggc gccgatcagg tcgacacata tctcggcgtg
     3901 ttcttcaccg acgacaagac gccgcgcaag gccgtgctga ccaaaagatt ttgcgacgac
     3961 aacccctcgc tcgcgcggtt gttcgatgcg gaatgcgctc gcctgcccgc gctgatcgaa
     4021 aaacgccgcg cgctcgtcgt gcgcgaccgc accgaagccc tgctgcatat cgctaccgcg
     4081 gcggcagcga attaccggcg tgagaaggag gagcgcgggc tgctcgatta tgacgacctc
     4141 atcgacaaga cgctgtcgat gctcaggcgc gtctcgccgg gctgggtgca ttacaaactc
     4201 gatcgcggcg tcgaccatgt gctcatcgac gaggcgcagg acacgagccc agggcaatgg
     4261 gacatcatcg accacatcat ctccgagttc acggccggca gcggcgcgcg cgacggcgtg
     4321 acccgcacca tctttgcggt cggcgacgag aagcaatcga tcttctcgtt ccagggcgcc
     4381 gcaccgcgcg aattcgacca gcgccgccgc acgttgcagc ggaaattcac ggacgccggg
     4441 ctgacatttg atccggtgtc gttcacctat tcgttccgct ccggaccggc gatcctgcaa
     4501 tccgtcgatc acgtgttccg cgatgaggcg atctatcgca gcattcattc ggtcgacatc
     4561 ggctatccga tccaccattc gcttgccgat gccgggccga gcctgatcga tttctgggag
     4621 cttgcccaag ccgacgaccg gaacgacatc gagggctggc gcgcgccgtt cgacggcgtc
     4681 tccatcacca gcggcgaagt caagctcgcc cgccgcatac aggcggagat caagcggctg
     4741 atcgacggcg gcaccatgac gggccccgcc ggcaaccgca ggcggctttc ccatggcgac
     4801 gtgctggtgc tggtgcgccg gcgcggcaac gttttcgacg cggtgatcca ggcgctgaag
     4861 cacgccggcg tcccggtcgc cggcgccgac cggctgaagc tgaccgagca catcgcgatc
     4921 atcgacctga tgaacctcgc cgacgcgctg ctcttgccgc aggacgatct tgcgcttgcg
     4981 gtcgcgctga aaagcccgct gttcggcctt accgacgacg acctgttcgc gctcgcctgg
     5041 gagcgcaaga caagcttgcg tgcgtcgctc gcgcagcaag cggcgacgaa cgagaaattc
     5101 cgaaatgtcg agaacaggct cgcgcagtgc gagcgctatt ttgcgcatat gacaccgttc
     5161 gcgttctacg cctggctgct cggcggcgac ggcggaagag cgaggatcct gaagcgcctc
     5221 ggtcatgagg ccaatgacgc gctggatgaa tttctcgaac tcgccctgaa ctatgaacgc
     5281 aaggccccgg cgtcgctgca aggctttgtg gcctggctgc gcgcagccga caccgaggtg
     5341 aagcgcgaca tggaaatctc gcgcgacgaa gtgcgcgtca tgacggtgca cggcgccaaa
     5401 ggactggaag catcggtcgt gtttctggtc gacaccacct catcgccgtc ggacacgcag
     5461 cggctgcggc tgatccagtt gccgcgcggc aatgacggag aggtcgtggt ctgggccggc
     5521 aagatggccg acgatcccaa agtggtcgcc aacgcgcgca gggcgatgct tgaggaaacc
     5581 gaggacgaat atcgccgtct gctttatgtg gcgatgacgc gcgccgccga ccggctgatc
     5641 gtcgcaggct gcaagcccgg caacaggaac aatgtgcgaa agctctgctg gtacgatctg
     5701 atcgagacgg gtctcacgcg ctccggcctc aacgagcaga ttatcgacac ggctgaaggt
     5761 ccggtgaagc gctttgcaca ttcggaagat gtcgcggcca atgcgggcgc tgcggccgcg
     5821 acggtcgggg cggtacgaag cgagcttccg gcatggttgc gcgctccgct cgggccgcaa
     5881 gcgtcgaacg cggcactgct gcgtccctcc gatgccggcg ctggcagtca cgggatcaga
     5941 acgagtgaga cgcttttcga gcgggcgcgg gctttgcagc gcggcacgct ggtgcatcgt
     6001 ctgttgcagt cgctccccga catcgccgtc gcgcgacgtc gtgacagcgc gatccgattt
     6061 cttgagcgca acgcggcggc atggagcgca ggcgagcgtg aagagctcgc acaaaaggtg
     6121 ctgggcctga ttgccgatcc tcgctttgcg cctgtgttcg gcgaaggcag tcgcgccgaa
     6181 gtcgccatcg tcggacggct cgcgctgccg ggacggcccg aggcgctggt gtccggacag
     6241 atcgaccggc tggtggtgac ggacaacga
//
LOCUS       DATLJT010000642         2622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3485494,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000642 DATLJT010000000
VERSION     DATLJT010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485494"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..502
                     /locus_tag="VM910_14875"
     CDS             326..502
                     /locus_tag="VM910_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63850.1"
                     /translation="MTHDERKMLMPTWPVRFDGVPTKVEPAPLLGEHTTEVLSDWLGL
                     DAAAVAQLRQDGIV"
     gene            773..2083
                     /locus_tag="VM910_14880"
     CDS             773..2083
                     /locus_tag="VM910_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63851.1"
                     /translation="MTDGLSTLYQDLLGGSYDCVDRIVLNAYFRTGHDPGGFRLWWRA
                     LTGSDETLENAYLMRLAGRFSRRVRGYARAHGIPLIDCPAGQRKHELAEEYLAKTNIT
                     HGLFLVLVARARAPVWDVSANHHIQRKQPIPFVNHYSFHILDRDWGHLTIKISGHPPF
                     PAQVMLNGHEYVACQARKAGTVFTKEGNCFTSISDAAGLAKIADTLSEHRAIGRLSQV
                     CERWIYRCVCFALDDDEQKRSGFRYQYSNYQIEYSRNLVFQVGAHMDQVFQALIDRSR
                     ALLDLKTIKTILGYKHRPKNRRRPAKWEVAVERPVYDLTIFKLHCGKLTLKIYTKAER
                     VFRIEAVAHNTQELKCGRSLDKFSELVSRLKAILERFADALSCIDQCFIADEMLEQLP
                     LPTQVGKTFVGGIDLNKARMRHVVEALIALSPSPNGFTASDLAA"
     gene            complement(2111..2287)
                     /locus_tag="VM910_14885"
     CDS             complement(2111..2287)
                     /locus_tag="VM910_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63852.1"
                     /translation="MGRSQQGLDGVVSENDERSHRPETGAKRLVAAGVADAANDLFAT
                     EFLEIVSGMPGTVL"
     gene            complement(2436..>2622)
                     /locus_tag="VM910_14890"
     CDS             complement(2436..>2622)
                     /locus_tag="VM910_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63853.1"
                     /translation="GEAMTGSDEASRIDAIAFFNRASPMLPSTITMLQFGTTIRRFGS
                     TRISPRPSPPVVLPPVI"
BASE COUNT          544 a          875 c          712 g          491 t
ORIGIN      
        1 ttatcactcg aagcgtggcg cgtgccgggc tcggcggtga aaaccaggcc tgtcgccgag
       61 cctcccataa ccgcttcacg cgaccgtggc tggaccagtc ggctggcacc ccgttccata
      121 aacgacacgc aataagagtc gaacccgggc gcccgttgca ccgactccgt cgccaggctg
      181 tcggcggcac gcgaagcccg tatcgtgccg acagtcggga tccaaaactc cgagtggaag
      241 ctctccggtt ccagccttag gcgccgctcg tcgagcgctg cgaattcgat cgagcagaac
      301 tggcgttcgt cgagccctgc agcagatgac gcacgacgaa cgcaagatgt tgatgccgac
      361 ctggccggtg cggtttgacg gcgtgcccac taaggtcgag ccggcgccgc tgctcggcga
      421 gcacactacc gaggtgctgt ccgactggct cggcctcgac gctgcggcgg tcgcacagct
      481 gaggcaggat ggcatcgtct gaggccccct cgccaacagc cgcttccatc accgccccga
      541 ccatgctcat caaccggcca gcaaacttga agccgcctac caccgcgccg acaacgccat
      601 ccaacagatc ctcgacctgc tcgccgcctg actacctccc gaatttcctc gacccttctt
      661 gtctacgatt ccctgggcaa gaatctacgc tttttcgcag acacactgcg aaaaagtggc
      721 ccctgcgggg cagtttgaaa cgggcggccg gctgcctgta acctgcccct tcatgaccga
      781 cgggctgagc actctctatc aggatttact gggcggcagc tacgattgcg tcgaccggat
      841 cgttctcaac gcctatttcc gaacggggca tgatcctggc gggttccgac tgtggtggcg
      901 agcgctgacc ggatcggacg agacgctcga gaacgcctac ctcatgcggc tggccggccg
      961 gtttagtcgc cgagtgcgcg gttacgccag agcgcacggc atccctctaa tcgactgtcc
     1021 ggcagggcag cgcaagcacg agcttgccga agaatatctg gccaagacga acatcacgca
     1081 cggcctgttt ttggtcctgg tcgctcgcgc ccgcgcgccg gtgtgggatg tcagcgccaa
     1141 tcatcacatc caacgaaagc aaccgatccc ctttgtcaac cactactcgt tccacatcct
     1201 cgaccgagac tggggccacc tgacgatcaa gatcagcggc caccctccct ttcccgccca
     1261 ggtgatgttg aacggccacg aatacgtggc ctgccaggcg cgcaaggcgg gtaccgtctt
     1321 caccaaagaa ggaaactgct ttacaagcat ctccgacgcc gcaggcttgg cgaagatcgc
     1381 agacaccttg tccgagcatc gggctatagg gcggttgagc caggtctgcg agcgttggat
     1441 ttaccgctgt gtctgcttcg ccctcgacga tgacgagcag aagcgcagcg gcttccgtta
     1501 ccaatactcc aactaccaaa tcgaatacag tcgcaacctg gtcttccagg tcggcgcaca
     1561 catggaccaa gtgtttcagg ccctgatcga ccgcagccgc gcgctgctcg acctgaaaac
     1621 catcaagacc atcctcggtt acaagcatcg cccgaaaaat cgccggcgtc cggccaaatg
     1681 ggaagtcgcg gtcgagagac ctgtgtatga tctgacgatc ttcaagctgc actgcggcaa
     1741 gctgaccctg aagatttata ccaaagccga acgcgtgttt cgcatcgaag cggttgccca
     1801 caacacgcag gagctcaagt gtggccgctc actggacaaa ttttcggaac tggtgtcgcg
     1861 gctaaaagcc atcctcgaaa ggtttgccga tgctctgagc tgcatcgatc aatgcttcat
     1921 cgctgacgaa atgctcgagc agctcccact ccccacacag gtcggcaaga ccttcgttgg
     1981 tggcatcgac ctcaataagg cacggatgcg ccacgtcgtc gaggcgttga tcgccttgtc
     2041 cccctcgccc aacggcttca ccgcctccga tctcgccgcc tgagtgcgcg catcgagcaa
     2101 gcaaacccca ctacaatacg gtccccggca tgccgcttac gatctcaaga aactccgtgg
     2161 caaacagatc attcgccgca tcggccacac ccgccgctac gagccgcttc gcaccggtct
     2221 ccgggcgatg actgcgctcg tcgttctccg aaacaacgcc atcaagcccc tgctggctgc
     2281 ggcccaacca ctccacccaa tgcgtggtcc gcacaaccca agaccaatcg actcgcatta
     2341 ccaaccatac aagttgcaat gcaaggcgtc ttccatgagc tcggactcgc cgcttgacat
     2401 ccacaatttt tttgtctggc tccgtcttga agtgcctata tcaccggagg cagcaccacg
     2461 ggcggcgaag gcctcggcga aattcgggtt gagccgaatc gtctgatcgt agtcccgaat
     2521 tgcagcatcg taatggtgct tggcagcatg ggcgaggccc gattaaagaa agcaatggca
     2581 tcaattcggg atgcctcgtc gcttcccgtc attgcttcgc cg
//
LOCUS       DATLJT010000643         4170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3714681,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000643 DATLJT010000000
VERSION     DATLJT010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3714681"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            910..1293
                     /gene="fliJ"
                     /locus_tag="VM910_14895"
     CDS             910..1293
                     /gene="fliJ"
                     /locus_tag="VM910_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HVI63854.1"
                     /translation="MKSRETLIRLKKFQVEDRRRTVAQIESMISGFERISADLERDIK
                     IEQDRSGIHDPAHFAYPTYAKSAIQRRDNLKRSAHDLKIQLEDAKAALGEAFEEMRKV
                     ELLGERDQIRERTEENLPGPSERVA"
     gene            complement(1328..2617)
                     /locus_tag="VM910_14900"
     CDS             complement(1328..2617)
                     /locus_tag="VM910_14900"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI63855.1"
                     /translation="MDRENKLAQRQRSSACSKPAATQRNSGQAGPIYIEPRNEPGNYD
                     REVFLVLKEFEPTLSRGGDMAQDFLAPAAHDKALEEAGEAAMRASLAKGMPHGFEVGY
                     GLFSINGRMLGQGEPVRVKLGERVLFHVLNASATEIRSVALPGHTFRVVALDGNLVPT
                     PAEVPVLWIGTAERVSAIVEMNHPGVWVMGDLADDDRDHGMGIVVEYAGRTGKPRWSA
                     PKPSKWDYTRFGKPGTAAAMPDETIEMIFEKNNAALDGFNQWTINGTPFSMETMEPKL
                     PIRQGRRYRLHMRNASDDIHPVHLHRHRLRVDRDRRESHGWRAQGRCDAGRLSGGGGG
                     LHSRQSRAHTVPLPPATAHGLRFHGVVRLCVKAAYIRCKTMARKKLKWQNIDADDLPA
                     EVKRSFNAMVEAEATFKADLEKLLKTSLDRLARASRT"
     gene            complement(2742..3734)
                     /gene="cydB"
                     /locus_tag="VM910_14905"
     CDS             complement(2742..3734)
                     /gene="cydB"
                     /locus_tag="VM910_14905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVI63856.1"
                     /translation="MEPSNLALFWAGVLACSILLYVFLDGFDLGVGVLFGTTRNEAHR
                     VRMMDTIAPFWDGNETWLVVIGAGLFAAFPDVYAVFLGAFYFPVLLLLFGLIFRGIAF
                     EFRFRSERMRRVWDWGFFLGSTVVAFVQGAAVGAMMRGIPVADGQYAGGPFGWLHPFA
                     ILTGVGLVLGYALLGAGWIVLKSEGELHDWAHRRIRWLAASLFAVLFLAFTVTFDYSV
                     LARSNFYARPWALIFPAIGVLALVGILVGAHRQRDALPFAMTGLFFVAAFLSLGAMFW
                     PYMIPYSITVASAAAPDESLQFLFYGAVLVLPVIAAYTIGVYWVFRGKVGMADG"
     gene            complement(3741..>4170)
                     /locus_tag="VM910_14910"
     CDS             complement(3741..>4170)
                     /locus_tag="VM910_14910"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVI63857.1"
                     /translation="GMGLIMLAVSWFGNFLRLRGQLETTRWFLWGTFLAFPTGFIAIL
                     TGWFTAEVGRQPWVVYGLLRTRDAVTPSLAIGDVLVSLAGYVLVYATFVSFGVYYIYK
                     LLREGPTAEAKAIPRATGSRPMAFAGTADSATGGRLRTEG"
BASE COUNT          842 a         1316 c         1279 g          733 t
ORIGIN      
        1 cagcgccaat cccgatcact tcggacatgg ctcagtatct cctgatggcg gagcgtgtac
       61 gtgtgggcag cagatgttac cttcgagcca atgcattgtg aattgtccta agcaccggtg
      121 gtcgctgagt gatcgtttca gcggtggctg tggggccgcc ccgatcgatt tcaagagctt
      181 acgattcgcg ccgcgaacca tgtcgccctc agcacgacgg cgataccgcg attgcctcca
      241 ctttcaaagg tgccccctca cgcgcaagtt tcgggacctc gaaagtggtg ctggcggagg
      301 cgctacgaca ttgacaacct tgtcgcgcgc ctcgcgatag atcgaaagcg cgcgcggatg
      361 tcagtgacaa aggcgctaaa ttttccattt tgccgccgac gagctgccgg ttggactgat
      421 gctcgtcgcg cgcaatgggc gcgatcgccg gttgttccga atggcagctg cggtcgaacg
      481 gctttttggg gcgtgacaaa tagtcggttc gttaccgtgt tgcggcatgc atcatttcca
      541 cctggatcgc ctcgtagagc gcgtttgccc caaggcccta gccccctgac cgcgccgcaa
      601 accgatcgac ggtgttcggt atgaggcggc gcgagttcat cacgctgctc ggcgcgaccg
      661 ttgtgtggcc gcttgcgcag ccgcgcagtt gacgccgccg ccgacccacg cgacgacact
      721 gaacccgcca cagatcaagg tggtgcgcgt gaagcctgga tttaccatgt cggacacccg
      781 ggcccttgtg tggtaatggc gccacggcga ggtaaggaag cagcaatgag ttcttgggca
      841 aatgttctgc cacggcgcgc ggaggcttcg ctgcgattag ggccggggct tgggaggagc
      901 acgaagtcga tgaagtcacg cgaaacactc attcgcctga agaagtttca agtcgaggac
      961 aggcgccgca cggtcgccca gatagagagc atgatcagcg ggttcgagcg catcagcgcc
     1021 gatctcgagc gcgacatcaa aatcgagcag gatcgctccg gcatccacga tccagctcat
     1081 ttcgcctatc ccacctatgc caagtcggcg atccagcgcc gcgacaatct caagcgctcg
     1141 gcccacgacc tgaaaattca gctcgaggac gccaaagcgg cgctcggcga agccttcgag
     1201 gagatgagga aggtcgagct gctcggcgaa cgcgaccaga tccgcgagcg gaccgaggaa
     1261 aatctccccg ggccgagtga gcgagtggca taatgcgcgg cctcagggcg cgaggcaacg
     1321 accggggcta ggttcgactc gccctcgcca accggtcgag cgacgtcttc agcagctttt
     1381 ccaaatcagc cttgaacgtg gcctcggctt caaccattgc attgaaagac cttttgacct
     1441 cggcgggcaa atcatctgca tcgatgttct gccacttcaa ttttttccgt gccattgttt
     1501 tgcaccgaat gtaggctgct ttcacgcata atcgaacaac gccatgaaac cgaagtccat
     1561 gtgcagttgc tggtggcagt ggaacagtgt gagcccggga ttgtcggctg tgaagtccac
     1621 ctccacctcc tgatagccgc ccagcatcac aacgtccttg agcacgccag ccgtggcttt
     1681 cccggcgatc cctgtcaact cgaagcctgt ggcgatggag atggacggga tgaatgtcgt
     1741 cgctggcgtt gcgcatgtgc agccggtagc gccgtccctg gcggatcgga agcttcggct
     1801 ccatcgtctc catggagaac ggtgtgccgt tgatcgtcca ttggttgaaa ccatccagcg
     1861 cggcgttgtt cttctcgaat atcatctcga tcgtctcatc aggcattgca gccgcggtac
     1921 ccggcttgcc gaaacgggtg tagtcccact tggacggctt gggtgccgac catcgcggtt
     1981 tgccggtccg gccggcgtac tcaaccacaa tccccatgcc gtggtcgcgg tcgtcgtccg
     2041 cgagatcccc catgacccag acgcccggat ggttcatctc gacgatcgcg gagacgcgct
     2101 cggcagtgcc gatccacagc accggcacct cggcgggcgt aggcactaag ttaccatcga
     2161 gcgccacgac tctgaacgta tgcccgggca gcgctacact gcggatttcg gtggcgctgg
     2221 cgttgaggac atggaagagc acgcgctcgc cgagtttgac gcgaaccggc tcgccttgac
     2281 cgagcatgcg accgttgatc gaaaacaggc cgtagccaac ctcgaaccca tgcggcattc
     2341 ctttcgccag ggatgcgcgc atcgcggctt cgccggcctc ttccaatgcc ttgtcgtgcg
     2401 ccgcaggcgc gaggaaatcc tgcgccatgt cgccgccgcg gctgagcgtt ggctcgaact
     2461 ccttaagcac gagaaagacc tcgcgatcgt agtttccggg ctcgtttctt ggttcgatgt
     2521 atatgggacc ggcctgtccg ctatttctct gcgtcgccgc gggctttgaa caggccgacg
     2581 accgttgtct ctgggctagt ttgttctccc gatccatcag cagaggtttt caccatggat
     2641 acgctcctgg gttgggcagc acgaagccca gaagtccccg ggaagccgtc gcatgattcc
     2701 aagtgccgat ttgcaacaac gccatcgcga tcctcgcaaa atcagccatc ggccataccc
     2761 accttgccgc ggaaaaccca gtagacgccg atggtgtaag cggcgatgac cggcaggacc
     2821 aacaccgcgc cgtagaacag gaattgcagt gactcgtctg gcgccgctgc gctcgcgacc
     2881 gtgatagagt agggaatcat gtacggccag aacattgccc ccaggctcag gaaggcggcg
     2941 acaaagaaga gcccggtcat ggcgaacggc agtgcatcgc gctgcctgtg tgcgcccacc
     3001 aggattccaa cgagcgcgag cacaccgatc gcggggaata tgagcgccca cggccttgcg
     3061 tagaaattgc ttcgcgcgag gacgctgtag tcgaacgtga cggtgaacgc cagaaacagg
     3121 accgcaaaca agctggcggc gagccaccga atgcgacgat gagcccagtc gtgcaactcg
     3181 ccttcgctct tgagcacgat ccagccggcg ccgagcagcg cgtagccgag cacgagcccg
     3241 accccggtca ggatcgcaaa cgggtgcagc cagccgaacg ggccaccggc gtactgaccg
     3301 tccgccaccg gaatgccgcg catcatcgcg ccgaccgcgg cgccctggac gaacgcgacc
     3361 accgtcgagc cgaggaagaa tccccagtcc cagacgcggc gcatgcgctc gctgcggaag
     3421 cggaactcga acgcgatgcc acggaagatc aggccgaaca gcagcaacag cacggggaaa
     3481 tagaacgcgc cgaggaacac cgcgtagaca tccggaaacg ctgcgaataa cccggcgccg
     3541 atcaccacca gccacgtctc gttgccgtcc cagaacgggg cgatggtgtc catcatccgt
     3601 actcggtgcg cctcgttccg ggtcgtgccg aacagcaccc caacgccgag atcgaagccg
     3661 tccaggaaga cgtagagcag gatcgagcat gcgagaacgc ctgcccaaaa caacgccaga
     3721 ttgcttggct ccattggcgc ctacccttcg gtccggagcc tgccgccggt ggcggagtcg
     3781 gcggtgccgg cgaacgccat cggtcggctg cccgtggctc tcgggattgc ctttgcctcg
     3841 gcggtcggcc cctcccgcag aagcttgtag atgtagtaga cgccgaaaga gacgaatgtc
     3901 gcgtagacga gaacgtagcc ggcgagcgag accagcacgt caccgatcgc gagcgagggt
     3961 gtgaccgcgt ccctggtgcg cagcagtccg tacacgaccc atggctggcg cccgacctcg
     4021 gcggtgaacc aaccggtgag gatagcgatg aagccggtcg ggaaggcgag aaacgtcccc
     4081 cagagaaacc agcgcgtcgt ctccagctgg ccgcggagcc gcaggaagtt cccgaaccac
     4141 gagacagcga gcatgatgag ccccatcccc
//
LOCUS       DATLJT010000644         1561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1417145,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000644 DATLJT010000000
VERSION     DATLJT010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417145"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..855
                     /locus_tag="VM910_14915"
     CDS             337..855
                     /locus_tag="VM910_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HVI63858.1"
                     /translation="MTISMYKASVPVFVQFLTSLSAVLDKAAAYAEAKKVEPSVLLNT
                     RLAPDMFPLVRQVRAATDHAINACGRLAGADMPSFSNTETTIPELKDRIAKTIDFLKS
                     LKPAQIDGSEDKAIEITFPSGATREFTGQSLLLTNSLPNFYFHCTTAYDILRQCGIEL
                     GKRDFMGTPVSL"
     gene            1365..>1561
                     /locus_tag="VM910_14920"
     CDS             1365..>1561
                     /locus_tag="VM910_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63859.1"
                     /translation="MCDYSLHHVATRPAQIEDKLVTTKFSNSITRGFAAVGEPHVAVC
                     LLPGTEIAFDENVECEPSFGVG"
BASE COUNT          297 a          486 c          475 g          303 t
ORIGIN      
        1 tgatgaggcc tcggttcgcg gactccatca gggccagcag ttcatccgga ctgcgtgaac
       61 aggccggaca catgactgca agcgacttct gtgcggcacg ctacattttc tcttgccagg
      121 cgggggccat ccacacatga accgccgcga cttcatcacg ctgctcggcg gcgcggctgt
      181 gacgtagccg ctcgcggcgc gcgcgcctgc tggcgcgggg atttttcgca tccgcgccgt
      241 cggctgatga gttggattcg gcatgagaca ctttcaggca tcggcaaagg ccgttaacct
      301 cgcttgacca gacgcatgat cgaagggtca cacgccatga ccatctccat gtacaaagcc
      361 tcggttccgg tcttcgtgca attcctcacc agcctgtcgg cggtcctaga caaggcggcg
      421 gcttacgccg aggcgaagaa ggtcgaaccg tcggtgctgc tcaacacgcg gctcgcgccc
      481 gacatgtttc cgctggtgcg gcaggtgcgc gcggcgaccg atcacgcgat caacgcctgc
      541 ggccggctcg ccggcgcgga catgccgagt ttctccaaca cggagacgac cattccggag
      601 ctcaaggatc gcatcgccaa gacgatcgat ttcctcaaga gcttaaagcc ggcgcagatc
      661 gacggcagcg aggacaaggc gatcgagatc acgttcccga gcggggcgac gcgcgagttc
      721 accggccagt cgctgctcct caccaattcg ctgccgaatt tctatttcca ctgcactacc
      781 gcctacgaca tcctgcgcca atgcgggatc gagctcggca agcgcgattt catgggcacg
      841 ccggtctcgc tctagcgcgc gtcccggttt ggtggaatcc ggaatccgtg caccgtccgc
      901 tcttcccgcg gaagcgggga ccccgatcac gagccgtgag caggtggctg tggattcccg
      961 cttgcgcggg aatgaacgga agaggtgctc cggcgtcgac ttggttcagg tgcatgccgg
     1021 tgacccgctt ttcagcggct tcgccacggg aagcaggtac tccattcata gcccgcccac
     1081 aaagcatgag gtctccgatc accatggacg ggctttgtcg ccaacgaccg tcatgacggt
     1141 catgttctcg aatatggtcc acgacagagc gcgtctgggc ttccgagcaa tccacaaaat
     1201 ggataatatt cgcttcctgt cgggagtcat ctgggggaac gcctaggtga tgagatggcg
     1261 acgttgctgg caagtcggga caacatccag caaatgacac cctgcggcgg tttgttgagc
     1321 cggacaacca tcccggcttt tcacaggaaa aggagtttcc ttcgatgtgt gactatagtt
     1381 tgcaccatgt cgcgacccgg cctgcccaga tcgaggacaa gctggtgacg accaagttca
     1441 gcaattccat cacccgcggc ttcgccgcgg tcggggagcc gcatgttgcg gtctgtctcc
     1501 tgccgggcac cgaaattgcg ttcgacgaga atgtcgaatg cgagccgtcg ttcggcgtcg
     1561 g
//
LOCUS       DATLJT010000645         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3944271,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000645 DATLJT010000000
VERSION     DATLJT010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3944271"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VM910_14925"
     CDS             complement(<1..546)
                     /locus_tag="VM910_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63860.1"
                     /translation="MQTVLVTGAAGGIGTRLRKLLKGVYPQLRWSDIRPPFDLSRDET
                     FVQADLAELAQVESAVAGVDGIVHLGGVSVEHPWDAVLNANIVGCYNLFEAARRQKVK
                     RIVFASSNHAVGFYPRKRKIGVDEPVRPDSRYGVSKAFGEAIGALYAYKHGMRVTCLR
                     IGNFGDAPVDHRRLAIWLKPDD"
     gene            complement(584..1345)
                     /locus_tag="VM910_14930"
     CDS             complement(584..1345)
                     /locus_tag="VM910_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HVI63861.1"
                     /translation="MSSSPTKSAQQLSATRPLVAPHSLTHQLAAQLTADIVEGRLASG
                     SRLPTEQEMVLANGVSRTVVREAVAALRANGLVVVRQGVGAFVAANVRRPFQIDVDGL
                     RSLRQALDVMELRMGIEIEAAGLAAERGTAAQIRKIVHAYEAVDLAIERGETAIDEDF
                     AFHVSIAEATGNPQFPRFLEYLGRSIIPRKTVRVNSPNMPNNRAYLRTFQEEHGEILG
                     AIRARTPTQARASMRRHLANSRKRYKKLAAEIGEE"
     gene            1484..>2026
                     /gene="dctP"
                     /locus_tag="VM910_14935"
     CDS             1484..>2026
                     /gene="dctP"
                     /locus_tag="VM910_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HVI63862.1"
                     /translation="MNRRSVIRLGAFTALAATTRGFVPSASAQSKMMFKASDVHPAGY
                     PTVVAVENIGKKLETATNGRLGVQMFAAMQLGGEKEAIEQAQIGAIQFARVSVGALGP
                     VIDDLNVFNLPFLFRNTAHMQRVIDGPIGEELLGKVTSNQKAGLVGICWMDAGARSVY
                     DTKKPIKEIADLKGMKVRVMG"
BASE COUNT          384 a          640 c          630 g          372 t
ORIGIN      
        1 gtcgtcgggc ttaagccaga tcgccagtcg ccggtgatcg acaggggcat cgccgaagtt
       61 accgatgcgc aggcaggtga cgcgcatccc gtgcttgtag gcatagagcg cgccgatcgc
      121 ctcaccaaac gccttgctaa cgccgtagcg tgagtccggg cgtaccggct catcgacgcc
      181 gatcttgcgt ttgcgcggat agaagcccac ggcatggttc gaggaggcga agacgatgcg
      241 tttgaccttt tgccggcgtg ccgcttcgaa caggttgtag cagccgacga tgttggcgtt
      301 gaggacggca tcccacggat gctccaccga gacaccgccg agatgcacga tcccgtccac
      361 gcccgccacg gcgctctcga cttgcgccag ctcggcgagg tcggcctgca cgaacgtctc
      421 atcgcgcgac agatcgaacg gaggacgaat atcgctccac cggagctgcg gatagacgcc
      481 cttgagcaac ttgcgcaggc gcgtgccgat tccaccggcc gcgccggtta ccaggactgt
      541 ttgcatcgct ccctcgcgtt gcatgaagta gcagggctag aaatcattcc tcgccgatct
      601 cggctgccag cttcttgtag cgctttcggc tgttggccag atgccgccgc atggatgcgc
      661 gcgcctgcgt cggcgtcctg gcacggatgg cgccgaggat ctccccgtgc tcctcctgga
      721 aggtcctcag gtaggcgcgg ttgttcggca tgttgggcga gttcacgcga acggtcttgc
      781 ggggaatgat ggaacggccg agatattcca agaaccgtgg gaattgcgga tttcccgtgg
      841 cctcggcgat actgacatgg aaggcgaagt cctcgtcgat cgccgtctcg ccgcgctcga
      901 ttgcgagatc gacggcctca tatgcatgca cgatcttgcg aatttgtgca gccgttccgc
      961 gctcggccgc aagccccgcc gcctcgatct cgatccccat gcgcagttcc atgacgtcga
     1021 gcgcctggcg cagcgagcgg agtccgtcca cgtcgatctg gaaaggccgg cgcacattag
     1081 cggcgacgaa ggcgccaacg ccctggcgca caaccaccag gccattggcc cgcaacgcgg
     1141 caaccgcttc ccgcaccacc gtgcggctga cgccgtttgc cagcaccatc tcctgctccg
     1201 ttggcagccg tgatccggat gccagccggc cctcgacgat atcggcggtg agctgcgcag
     1261 ccagctgatg cgtcagactg tgcggtgcga cgagcggtcg ggtcgcggag agctgctgcg
     1321 cagatttggt cggcgaactt gacatgaggt agcttattat aggatcatcg tatgacctga
     1381 tggctaacat aaccctcgat gcaaatcaac ggccaggcct ccaagaccgg cccgtcataa
     1441 caagacgggg cgctcgcagc ctacctttgg gaggagatat gacatgaatc gccgtagcgt
     1501 gatccgcctg ggcgctttca ccgcgcttgc agctaccacc cggggcttcg ttccgtcggc
     1561 ctcggcgcaa tcgaaaatga tgttcaaggc ttccgatgtt catccggcag gttacccgac
     1621 ggtagtcgcg gtcgagaaca tcggcaagaa actcgaaacc gctaccaacg gccggctggg
     1681 tgttcagatg ttcgccgcga tgcagcttgg cggcgagaag gaagccatcg agcaggccca
     1741 gatcggtgcc atccaattcg cgcgcgtcag cgttggcgca ctcggcccgg tgatcgacga
     1801 tctcaacgtg ttcaacctgc ctttcctgtt ccgcaacacc gcacacatgc agagagtgat
     1861 cgacggaccg atcggcgagg aacttctcgg caaggtcacc agcaaccaaa aggccggcct
     1921 tgtcggcatc tgttggatgg atgccggcgc gcgcagcgtc tacgacacca agaagccgat
     1981 caaggaaatc gccgacctca agggcatgaa ggtccgcgtc atgggt
//
LOCUS       DATLJT010000646         5757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1647437,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000646 DATLJT010000000
VERSION     DATLJT010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647437"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..398
                     /locus_tag="VM910_14940"
     CDS             204..398
                     /locus_tag="VM910_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63863.1"
                     /translation="MGIIIEFPADAASRRSGSTVVGASHGVGTVLILPVVRIERDTDG
                     TSGGSGPEEGAAPGRRRRRR"
     gene            417..1178
                     /locus_tag="VM910_14945"
     CDS             417..1178
                     /locus_tag="VM910_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63864.1"
                     /translation="MPDTHVPIRSLLPVLLLLLGAALGGCSGGDFWRTRDDFRNDDMH
                     RWVGAEATASIGLRPSQFQLTDYERQLRDLAYPLIEPPHSRPAWKAVFGDYQVIASPW
                     RQSAPPFDRTAYGRMLIDEPHRSHASRYAQLIDDVRDDITRFEPFYATAIKVLELDRK
                     RNASLPHISELSPPEYADAVGRMQENSLIVQWVQQCLERRVSSYRWALERLVLQAPDN
                     IAADADRLIGELAAQTANPPVAARPVIERPLTTRG"
     gene            complement(1194..1805)
                     /locus_tag="VM910_14950"
     CDS             complement(1194..1805)
                     /locus_tag="VM910_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HVI63865.1"
                     /translation="MIRLLFTIVAGVLLGLVVHLVSVLALPRIASQDAYSRLTPTTQL
                     NAVTMLPAADPGYTLMPFMDPAFAVAVCRYDLSAGPIKLTVPVNQAYTSVSFYTRNEV
                     AYYAINDRSAGKKVIELNLMTEAQHDELPEDENITAADRLIIDSPTATGLIVMKALAP
                     EPGLMAQAQATLAASSCKVQVDQPAKQAEAPQPGSAPFRPSRR"
     gene            complement(1802..2380)
                     /locus_tag="VM910_14955"
     CDS             complement(1802..2380)
                     /locus_tag="VM910_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HVI63866.1"
                     /translation="MRLIFITLLALLVASVVGLGATWMTTTRGTDLGTLTIGSWTARP
                     RTGTSDVDPYARATITRNGELPVGTGDGVAFTATTDDRRKPLDGRCDVVVSGVTPAAR
                     FWTLTLYDRKGHLVANTLQRYGFTSQEIVRGADGSFEIRIASRSRAGNWLPTGGIERY
                     ALMLRLYDTPVGVATRTQRDAPMPAIATAGCP"
     gene            complement(2391..4673)
                     /locus_tag="VM910_14960"
     CDS             complement(2391..4673)
                     /locus_tag="VM910_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVI63867.1"
                     /translation="MRQILPPDWKTKIRHILLDLDARIDSTLFSSGKGARELYERYST
                     FMDRFYVGRWKRWVFIEPLSEAATLGLGGLILLLALAVPAFRETADEDWLKKSDLAVS
                     FLDRYGNPIGSRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDVAGTMRALVT
                     NAQAGGVRQGGSSITQQLAKNLFLSNERTIERKVNEAFLAIWLETRLTKNEILKLYLD
                     RAYMGGGTFGVDGAAHFYFNKSVRDINLAEAAMLAGLFKAPTKFAPHINLPAARARAN
                     VVLDNLVDAGFMTEGQVFGARRNPAFAVDRRDENSPNYYLDFAFDEMRKLVDTFPKSY
                     TERVFVVRTAIDMNVQHAADEAIENQLRQFGRDYHATQAASVVADLDGGIRAMVGGRD
                     YGASQFNRATDAYRQPGSSFKPYVYTTALLNGFKPTSVVVDGPVCIGNWCPQNYGHSY
                     SGSVTLTQAITRSINVVPVKLSIAIGGNPKNVWDSAKKGRAKIVETARKFGITAPLPD
                     TPSLPIGADEVTVIEHAVAYATFPNKGRSVTPHAVLEVRTGAGDLVWRWDRDGPKPRQ
                     AIPASVAADMAQMMSHVVSEGTARRAGLDGIPTAGKTGTTNSYRDAWFVGYTGNFTCA
                     VWFGNDDYSPTNRMTGGSLPAQTWHDIMTAAHQGIEIKELPGVGMGAKLPQPAVPQTV
                     SAAQPKPPEIKPGPPPVLTKRGADVLVQVEKMLDEAAKAADKTSTGAPPKPNRPVSSS
                     ALPFPDSFAAAAPNSTPSKD"
     gene            4961..5476
                     /locus_tag="VM910_14965"
     CDS             4961..5476
                     /locus_tag="VM910_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HVI63868.1"
                     /translation="MTAPPKPPSAQEGFFWKTKTLEEMSDVEWESLCDGCARCCLEKL
                     EDEDTGKIYFTHVSCRLLDAGLCTCKDYPHRNDKVPDCVRLTPENVRTLSWLPPSCGY
                     RLVAEGRDLYWWHPLISGDPNTVHEAGVSVRGRVRGSEDEIPDADLEDHIVHWPTLLP
                     KRARLKKRPKP"
BASE COUNT          996 a         1764 c         1959 g         1038 t
ORIGIN      
        1 taacagctcg ttaaggaaaa caaattgtga agattgaaga ttgaagtcgt ctctcgcgct
       61 gaggccgcgc tgcaacgcaa gccgcaatag cgcggccggt gtgcgtgccc gcaatgcaaa
      121 ttaagaagcc gttaaccacg ttttggtctt aatcaacgcg tcgcgggaca agcctgtcgc
      181 cgcggcaacc agagagaagg ggcatgggga tcatcattga atttccggcg gatgcggcgt
      241 cgcggcggtc tggctcaacc gtggttggtg cttctcacgg cgtggggacc gtcctgatcc
      301 ttccggtcgt tcggatcgag cgcgataccg atggaacgag cggcggcagc gggccggaag
      361 agggcgccgc accgggtcga cgtcgccgtc gacgctgaaa tgttcggcgc aacgccatgc
      421 cggacacgca tgttccgatc cgttctctat tgccggttct gctgctcctg ctaggggcgg
      481 cgcttggcgg ctgcagcggc ggcgatttct ggcgcacccg agacgatttt cgcaacgacg
      541 acatgcatcg ctgggtcggc gccgaggcga cggcgagcat cggcctgcgg ccgtcgcaat
      601 tccagctcac cgactacgag cgccagcttc gcgatctcgc ttatcccttg atcgagccac
      661 cgcattcgcg ccctgcctgg aaggcggtgt tcggcgacta tcaggtgatc gcgtcgccct
      721 ggcggcaatc ggcgccgccg ttcgatcgga cggcttacgg gcgcatgttg atcgacgagc
      781 cgcatcgctc acatgcgtcg cgctatgccc aattgatcga cgacgttcgc gacgacatca
      841 cgcggttcga accgttctac gccaccgcga tcaaggtgct cgagctcgac aggaagcgta
      901 acgccagtct cccgcatatt tccgaattgt cgccacccga gtacgccgat gcggtcggcc
      961 gcatgcagga gaactcgctg atcgtgcaat gggtgcagca gtgtctggag cgacgcgtct
     1021 cgtcctatcg ctgggcgctc gaacggctgg tgctgcaggc gcccgacaat atcgccgccg
     1081 acgccgaccg cctgatcggc gagcttgcgg cgcaaaccgc aaatccgccg gtcgcggcgc
     1141 ggccggtgat cgaacgcccg cttacgacgc gcggttagat ttattgtcga cccttatcga
     1201 cgtgagggac ggaagggtgc gctgccgggc tgcggcgctt cggcctgctt ggccggctgg
     1261 tcgacctgca ccttgcaact cgaggcggcc agcgttgcct gtgcctgcgc catgagaccg
     1321 ggctcgggcg caagggcctt catcacgatc aggccggtcg cagtcgggga atcgatgatc
     1381 agccggtcgg ccgcagtgat gttctcgtct tcaggcagct catcgtgctg cgcttccgtc
     1441 atcagattga gctcgatcac cttcttgccg gccgagcggt cgttgatcgc gtaataggcg
     1501 acttcgttgc gagtgtagaa cgatacggac gtataggcct ggttgacggg gacggtgagc
     1561 ttgatcggtc cggcggacaa atcgtagcgg cagactgcga ccgcaaaggc cgggtccatg
     1621 aacggcatca gcgtgtagcc gggatcggcc gccggtagca tcgtgacggc gttgagctgg
     1681 gtggtcggcg tcagccgcga ataggcgtcc tgcgaggcaa tcctgggcag cgccagcacg
     1741 ctaaccagat gcacgacgag ccccaacagc acgcccgcga cgatcgtgaa caagagccgg
     1801 atcacgggca ccccgccgtc gcaatcgccg gcatcggcgc gtcgcgctgc gtgcgcgtcg
     1861 ccaccccgac cggcgtgtcg tagagccgaa gcatcagcgc atagcgctcg atcccgccgg
     1921 tcggcagcca gttgccggcg cgcgagcgcg aagcgatgcg gatttcgaag ctgccatcgg
     1981 cgccgcggac gatttcctga ctggtgaagc cgtaacgctg cagcgtgttg gcgacgagat
     2041 ggcccttccg gtcatagagc gtcagcgtcc agaagcgcgc cgccggcgtc acgccgctga
     2101 cgaccacatc gcagcggccg tcgagcggtt tcctgcgatc gtcggtcgtg gccgtgaacg
     2161 ccacgccatc gccggtgccg accggcagtt cgccgttgcg cgtgatggtg gcgcgcgcat
     2221 agggatcgac atcggaggtg ccggttctcg ggcgtgcggt ccaggagccg atcgtcagcg
     2281 tgccgagatc ggtaccacgc gtggtcgtca tccaggttgc gccgaggccg accacggagg
     2341 cgaccagaag cgccagcaag gtaatgaaga tcagtcgcac ggcgacgggc tcaatctttg
     2401 ctcggcgtgg agttcggtgc cgccgccgca aagctgtcgg ggaaggggag tgcgctcgac
     2461 gagaccggcc ggttcggctt cggcggcgcg cccgtcgaag tcttgtcggc cgccttcgca
     2521 gcctcgtcga gcatcttctc cacctgcacc agaacgtccg cgccgcgctt ggtcaaaacc
     2581 ggcggcggcc ccggttttat ctcgggcggc ttcggctgcg ctgcggacac ggtttgcggc
     2641 acggccggct gcggcagctt ggcgcccatg ccgacgccgg gcagttcctt gatctcgatc
     2701 ccctggtgcg cagcggtcat gatgtcgtgc caggtctgcg ccggcagcga gccgccggtc
     2761 atgcggttgg tgggcgagta atcgtcgttg ccgaaccaga cggcgcaggt gaaattgccg
     2821 gtgtaaccga cgaaccaggc gtcgcggtag gagttggtgg tgccggtctt gccggcggtc
     2881 gggatgccgt cgagtccggc acgccgcgcg gtgccttcgc tcaccacgtg gctcatcatc
     2941 tgcgccatgt cggcggctac cgatgcaggg attgcctgcc gcggcttcgg accatcgcgg
     3001 tcccagcgcc agaccaggtc gccggcgccg gtgcgcactt caagcaccgc atgaggggtg
     3061 accgacctgc ctttgttggg gaaggtcgca taggcgaccg cgtgctcgat cacggtgact
     3121 tcgtcggcgc cgatcggcag cgacggcgta tcgggcagcg gagctgtgat gccgaacttg
     3181 cgggccgttt cgacgatttt cgcgcgtccc ttcttcgccg aatcccagac gttcttcggg
     3241 ttgccgccga tcgcgatcga cagcttgacc ggaacgacgt tgatcgagcg cgtgatcgct
     3301 tgcgtgaggg tcaccgagcc ggaataagag tggccatagt tctggggaca ccagttgccg
     3361 atgcagaccg ggccgtcgac cacgaccgag gtcggtttga agccgttgag cagcgcggtg
     3421 gtgtagacat agggcttgaa cgacgagccg ggctggcgat aggcgtcggt cgcgcgattg
     3481 aactggcttg cgccatagtc gcggccgccg accatggcgc gaatgccgcc gtcgagatcg
     3541 gccaccacgc tagcggcctg ggttgcgtga taatcgcggc cgaactggcg gagctggttc
     3601 tcgatcgcct cgtcggctgc gtgctgcacg ttcatgtcga tcgcggttcg gaccacgaag
     3661 acgcgctcgg tataagactt cggaaaggtg tcgacgagct tgcgcatctc gtcgaaggca
     3721 aagtcgagat agtagttcgg cgaattctcg tcgcgccggt cgaccgcaaa ggccggattg
     3781 cgccgcgcac cgaacacctg gccctctgtc atgaagccgg cgtcgacaag gttgtcgagc
     3841 accacgttgg cacgggcgcg cgccgcgggc aggttgatgt gcggcgcgaa cttggtcggc
     3901 gccttgaaca ggccggcaag catggccgct tctgcgaggt tgatgtcgcg caccgacttg
     3961 ttgaagtaga aatgcgcggc gccgtcgacg ccgaacgtgc cgccgcccat gtaggcgcga
     4021 tcgagataga gcttcaggat ctcgttcttg gtgagccgcg tctcaagcca gatcgcgagg
     4081 aacgcctcgt tgaccttgcg ctcgatggtg cgctcgttgg agaggaacag gttcttggcg
     4141 agctgttggg tgatcgacga accgccctgg cgcacgccgc cggcctgggc gttggtcaca
     4201 agcgcgcgca tcgtgccggc gacatcgatg ccgaaatggt cgtagaagcg gcggtcctcg
     4261 gtcgcaagcg tcgccttgat cagattatcc ggaaaatctt ccagcgggat cgagtcgttg
     4321 tgtttgatac cgcggctgcc gatcgggttg ccgtagcggt cgagaaagct gacggcgagg
     4381 tcggatttct tcagccagtc ctcgtccgcg gtctcgcgaa acgccggcac cgcgagcgcg
     4441 agcaacagga tcagcccgcc gagcccgagc gtcgcggctt ccgagagggg ttcgatgaag
     4501 acccagcgct tccagcggcc gacatagaag cggtccatga aggtcgaata gcgctcgtag
     4561 agctcgcgcg cgcccttgcc ggacgagaac agggtcgagt cgatgcgcgc gtcgagatcc
     4621 agcaggatgt gccggatctt ggtcttccag tcgggtggta ggatctggcg cacgaggact
     4681 attcgtcagg gttcgaaggc gttcgagcac ccacccgggc gctattccga cgtcttgcag
     4741 ggttgttgcg gcaaacccga ggcgcccgaa gcgcgccctg ggactcgcca tttgttctag
     4801 ccgagcgggg accataaacc aatggtcccg ccggactttt gatcgccaga actaacgttg
     4861 ttgtggggga acgccgcacg gttttattgg ttacttgcgc catcgccggc ccagccctta
     4921 gaagagccgg gcgaaaatcc tgaccgagct accgggagcc atgaccgcgc cgcccaagcc
     4981 gccctcggcc caagagggtt ttttctggaa aaccaagacg ttggaggaaa tgtccgatgt
     5041 cgaatgggag agcctgtgcg acggctgcgc gcgctgctgc ctggaaaagc tggaggacga
     5101 ggataccggc aagatttatt tcacccacgt ctcctgcagg ttgctggatg cgggactttg
     5161 cacttgcaag gactacccgc accgcaacga caaggttccg gattgtgtga ggctgacccc
     5221 ggagaacgtg cgaaccttga gctggctgcc gccgagctgc ggttaccggc tggtggcgga
     5281 gggccgcgat ctctattggt ggcatcccct gatctcgggc gaccctaaca ccgttcacga
     5341 agcgggcgtc tcggtgcgcg ggcgggtgag gggaagcgag gacgagatcc cggacgccga
     5401 tctggaagat cacatcgtgc actggccgac gctgttgccg aagcgggcgc ggctcaagaa
     5461 acggccgaag ccgtgacgcg cggcctcggg tcggctatca cgcagatgcg aacgggaccc
     5521 tcccatgcac ggaaggtgct gccgcgcagc aggtttgtta cctaaattgc tctgcattga
     5581 gtgattccgc gcgggttccg ccgcgtcaga cgaaaaaatc ctgatgaaca agttcgagcg
     5641 gcagagccgt aaggctgccg atcaagagac attgccggac gccatcgaag ctgagcttgc
     5701 cgagatcccg gcgcaggtga ttgcggtccg cgacgcaccc ccgatcgcgc cccgctc
//
LOCUS       DATLJT010000647         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2336199,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000647 DATLJT010000000
VERSION     DATLJT010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336199"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VM910_14970"
     CDS             complement(<1..444)
                     /locus_tag="VM910_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HVI63869.1"
                     /translation="MAAKEIRFHSDARERMLRGIDILANAVRVTLGPKGRNVVLDKSY
                     GAPRISKDGVTVAKEIELADKFENMGAQMVREVASKTSDAAGDGTTTATVLAQTIVRE
                     GARAAAAGMNPMDLKRGIDRAVEVVVKEIEKRSKKVSTNDEIAQVG"
     gene            1177..1404
                     /locus_tag="VM910_14975"
     CDS             1177..1404
                     /locus_tag="VM910_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63870.1"
                     /translation="MAAQTDELHAMIAALAGSQALLLDVLRRRGSIVQDDIDYILAVT
                     EQGIAKAVGTAGPQAVVQMIRLCLQQLSTSE"
     gene            complement(1455..1640)
                     /locus_tag="VM910_14980"
     CDS             complement(1455..1640)
                     /locus_tag="VM910_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63871.1"
                     /translation="MLSAKRMLTAKAVALVRLLTAAAAAQEAPPVTTVEIVALSLPGM
                     GIDRDKVPSNVQSLQTP"
     gene            complement(1856..2026)
                     /locus_tag="VM910_14985"
     CDS             complement(1856..2026)
                     /locus_tag="VM910_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63872.1"
                     /translation="MQNGELLTVTATDPKGNPAYQRLGFIGLLASSAHHQMHHLMMVK
                     GEFDYEHLAPRQ"
     gene            complement(2248..2436)
                     /locus_tag="VM910_14990"
     CDS             complement(2248..2436)
                     /locus_tag="VM910_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63873.1"
                     /translation="MTIGGNGPVIEAGLGSDVAVLFLLAAFVMTSFRTAGAAFGLPAV
                     FAAGLLRAAGAVFLTVLT"
     gene            2420..2719
                     /locus_tag="VM910_14995"
     CDS             2420..2719
                     /locus_tag="VM910_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVI63874.1"
                     /translation="MPPIVTLKHLAEWAGYNHGVAKKQAIEMFTGFVADIGRVLKKGS
                     KIRIPNLGVLQVRIRPARPARKGRNPATGEEIQIKASKASKKVAFRVAKGLSEAI"
     gene            2866..3549
                     /locus_tag="VM910_15000"
                     /pseudo
     CDS             2866..3549
                     /locus_tag="VM910_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3734..3892)
                     /locus_tag="VM910_15005"
                     /pseudo
     CDS             complement(3734..3892)
                     /locus_tag="VM910_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4288..>4827)
                     /locus_tag="VM910_15010"
     CDS             complement(4288..>4827)
                     /locus_tag="VM910_15010"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI63875.1"
                     /translation="VMLGSKAAAQSGSSCGARIADMSGRGSLSSLLDFAWRTAFRLGF
                     PLARIWWRLTRPRHQGVVVAVYVGPDLLLVRSSYRTGWHLPGGGVRRGETPEEAARRE
                     LAEEIGVTASALLPAGSTCGIWDGRRDRIFFFELRLVELPKLQLDNREVIEARLTSPI
                     ELQNMVLTDLVAIYLARRQ"
BASE COUNT          945 a         1527 c         1427 g          928 t
ORIGIN      
        1 gccgacctgg gcgatctcgt cgttggtcga cacctttttg gagcgctttt cgatctcctt
       61 gaccaccact tccaccgccc ggtcgatgcc gcgcttcagg tccatcgggt tcatgcccgc
      121 agccgctgct cgggcgcctt cacgcacgat cgtctgggcg agaacggtcg ctgtcgtcgt
      181 accatcgcca gcggcgtcgc tcgtcttcga tgcgacctcg cgcaccattt gcgcgcccat
      241 gttttcgaat ttgtcggcga gctcaatttc cttggcgacg gtgacgccgt ccttgctgat
      301 gcgcggcgcg ccgtaggatt tgtcgagaac gacgttgcgg cccttcgggc ccagcgtgac
      361 cctcaccgcg tttgccaaaa tgtcgatgcc gcgcaacatc cgctcgcgcg catcgctatg
      421 aaaccggatt tcctttgccg ccatggtctc ctcccctcac gcaagcctgc cgacgcagtc
      481 tattcaacta cgcccatgat gtcgctctcc ttcgcgacga cgaaatcctg cggtcgatct
      541 tgatctcggt gcctgacact ttgcgaacag cactcggttg gcgatcttga catcgagcgg
      601 ctggagctcg gctatcgtcg ccccgcggta gccgacgccg cagccggtaa gcttccgaat
      661 tctcctcatt ctgcgccgac caaagtggcg atcgacgaca aggacctcga gcactcagct
      721 gtccagaagc tgccgccgat ggctgccacg gtgtcacgcg ctgccagccc atgtgggaca
      781 agatcatgcg aacgggcaga cggtgatggt caacaacggc ttcgtccaca ttcacgagac
      841 cgcgccatgg ctcggcgggg atctgcacga aatgatcgtt tcccgaactg caagcacgac
      901 gaccaggtcg gtccaacggc gcagttcctc acctggttca ggcgccgtcc gcgggccaat
      961 agctgttcga gctcatgagg gatggacacg caagagctga ggaggaggct cgcagcccca
     1021 cccaatccgg ttctgctcca ctcaatggct cgtggcacag gaaaatcggc cttttttcct
     1081 acgtacaaca agcgggtatt gagcataaac gctctcagac ctagtgttaa gtctcgccct
     1141 cgatcacgct ggcccgtagg aatgagggaa acgcccgtgg ctgcccaaac agacgaattg
     1201 catgcgatga ttgcagctct ggctggttcg caggcgctac tattagatgt tttgcgccga
     1261 cgcggctcga tcgttcaaga tgatatcgac tacattctag ccgttacaga gcaaggcatc
     1321 gcaaaggcgg tcggcaccgc tggacctcaa gccgtggtcc aaatgatcag gctatgcttg
     1381 cagcagcttt caacttctga atagccgaca aagcgcgccg gcgtggtgaa ggacgtcggc
     1441 ccctgcgtcg tgatctatgg cgtctgcagg ctttgcacat tggacggcac cttgtcgcgg
     1501 tcgatcccca taccgggcag gctgagggcg acgatctcca ctgtcgtgac cggcggcgcc
     1561 tcctgcgccg ccgccgcagc cgtgagcagc cggaccagtg cgacagcttt cgcagtcaac
     1621 atgcgtttgg cgctcagcat ttccctccga tgacgtcgct tcttcggccg agcctatatt
     1681 gagcccgaaa tactgcgaag cgctttcccg atctggtcgg aaacttttcc cgacttagtc
     1741 ggatttttcg cgatctaggc agcgaaatgc ccgtcgaaag ttgacaagat ggtatcgtgc
     1801 gctgccgtgc tggccgcctg tccggcccac cgcacatctc ggaacacaag gccgtttact
     1861 gccgaggggc caagtgttca taatcgaact ctcccttgac catcatcagg tggtgcatct
     1921 gatgatgcgc gctgctcgcc aacaagccga taaagccgag ccgctgatat gctggatttc
     1981 ccttcgggtc ggttgccgta acggtcagaa gttcgccatt ctgcaactgg ctcgccttcg
     2041 tctccacccc tggtgaccgt tcgtcgcctc ggcccaagct ggaacacttt gccagtgatt
     2101 cgctccggcg gagagatcag cagggcagca cgcacatccc caccggtcgt ttccgctgcg
     2161 tttccacgca aagttgcagg ttcttgcaac gcgtcggtat tttctgctcc ggaaaggcaa
     2221 acttggagag ccctcgatgg ctacacctca ggtcaataca gtgagaaaga cagcacctgc
     2281 ggcacgcagc aagccagccg caaagacggc cggtaagccg aaggctgcac ccgcggtgcg
     2341 gaagctggtc atcacgaagg cggccaacaa gaaaaggact gcgacgtctg atcctaagcc
     2401 cgcttcgatg accgggccat tgccgccgat cgtcacgttg aaacatctgg ctgaatgggc
     2461 gggctataat cacggcgtag ccaagaagca ggcgatcgag atgttcaccg gcttcgtggc
     2521 cgacatcggt cgtgtgctga agaagggcag caaaatccgc attcccaacc ttggcgtcct
     2581 gcaagtacga attcgaccgg cacgcccggc gcgcaagggg cgtaatccgg ccacgggcga
     2641 ggagatccag atcaaagcca gtaaagcgtc aaagaaggtt gcattccgag tcgcaaaggg
     2701 tttgagcgaa gcaatctgat ttgccgttct ggccgctacc ctgttgtcag cggtaggagc
     2761 ttcacgccga tttcccgccg ttcgggaggc ggtgatcgag ccgctagacg gtggcctgga
     2821 gatcgcgctc accggcagcg ggcgcacgct cgttgccata taccggatgg catccgcttc
     2881 acgccggcaa tcctgccgcc ctacgcgcgg cgctcccgca gcctggaggt gctgatccca
     2941 atcctttacc tgaagggcat ctcgaccggc gatttcgagg agacgcttgc cgccctgctt
     3001 ggcagggatg cgccgggcct atcggcttcg acgatcgcgc ggctgaagga ggtctggctc
     3061 gacgagcacg agcattggag caagcgcgat ctgtcggcca agcgctatgt ctacgtgtgg
     3121 gccgacggca tttacctgca ggcccgtttt gaagacgcca agcaatgtat cctggtcatc
     3181 atcggcgcga cgccggaggg caaaaaggag ctgctcggct ttaccgacgg tgcgcgcgaa
     3241 ggcgcgcagg ataggcgcga gctgctgctc gatttacaga accgaggctt ggcggtcgcg
     3301 cccaaacgcg ccgtcggcga cggcgcgctt ggcttctgga aggcgctcgc cgagttgtgg
     3361 ccgaccacac gagagcaacg ctgctgggtg cacaagaccg ccaatgtcct caacaaactg
     3421 ccgaagagcc agcagccgaa ggccaagcgg tcattacaag agatctggat ggccgagacc
     3481 agcaaggacg ctgaggccgc gttcgacgcc agctcggcgc taattcataa cctgaccaat
     3541 acgctctgag aggcgaacgc ggttctcgat cgcgataagg actgcgttaa cgtcttacct
     3601 gtgggaaccc actgtggtac ggcaagccat tgaaacagcg tggactctgc accgtggatg
     3661 agctatcgcc gaattttggt gacggcctga aggaacacgc ggcggcttgg gcttgagggg
     3721 cggcacaatt gccgatgacc tcgatgccgt cggtgaactt cacaccctgg attagttttg
     3781 gcaactggtt gtggccgtca attcgtcgcc aacttttctg ggcgctgtcg atcagcttga
     3841 agaccatcgc gagcgccgtc ttattcgata ggcagccctt cgcacggatc gtcaggcgaa
     3901 cattcgttgg cagacatgat ttccaccagg gctgccgcct ttgatggccc ctcagctaca
     3961 actcgaatct acactcgctt gacggacgtg acccagcgac cgaccgcttg ctcttctagc
     4021 cttaaccggc ggcgatcact tggaatgacc tggagcggct actgatggtg ggtgcccgcg
     4081 ccgcgcgctg cctgcggctt gccaaatatg ggcgggtcgg tgttgccgcg tcgccccgtt
     4141 caccaccatg ctgacccggc ggctcccgca ctggccgcgc acaagcctcg ccgccgaatc
     4201 cggtacggtc gtctccgcgc cgcatcgccc cgccagccgg agcgctgcag agccgcggag
     4261 tcaagtcggg tctgggtggc agccgagcta ttgccggcgt gcgagataga tggcgaccag
     4321 gtccgtcagg accatgttct gcaactcgat aggtgaggtc agccgggcct ctatgacctc
     4381 tcggttatca agctgcagct ttggcaattc gaccaagcgc agctcaaaga aaaatatccg
     4441 gtcgcgccgt ccgtcccaaa tgccgcatgt agagcccgca gggagcagcg ccgacgcggt
     4501 gacgccaatc tcctccgcca gctcccgccg tgccgcctct tctggcgtct cgccgcgccg
     4561 cacaccgcca ccgggcaagt gccaaccagt ccgatacgag gagcgcacta gcaagaggtc
     4621 agggccgaca tagaccgcca cgacgacacc ctgatgcctc gggcgcgtca gtcgccacca
     4681 gatgcgagca agcggaaaac caagccggaa agctgtccgc caggcaaagt cgagaagtga
     4741 cgaaagcgag cctcgacctg acatgtccgc aatccttgca ccacacgatg agccggactg
     4801 ggccgccgcc ttgctgccga gcatcac
//
LOCUS       DATLJT010000648         6688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4404141,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000648 DATLJT010000000
VERSION     DATLJT010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404141"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VM910_15015"
     CDS             <1..191
                     /locus_tag="VM910_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63876.1"
                     /translation="IVDKFLQDDERPLRRAMPGLRDQFFLARKVEQARRTERLSLKTR
                     LSRRRMAGRAVPQAWPNL"
     gene            241..504
                     /locus_tag="VM910_15020"
     CDS             241..504
                     /locus_tag="VM910_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63877.1"
                     /translation="MNSKLVDLVDHPKLVNQLASLERRTARGGRDSIDHAPGAHDDIA
                     NAVSGVVGAISGVGVFDFEFWHDDRTWSGGAPSGDDLLWFLRR"
     gene            1144..1983
                     /locus_tag="VM910_15025"
     CDS             1144..1983
                     /locus_tag="VM910_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63878.1"
                     /translation="MPGFITLAIPRPARAPTIAPGYGWHPLLSFAASERNRLRLQAAR
                     RVNEWLKTDPDLTLIVPIKERSLEIFGDEKRLDELRGGTTMLFGQLALGAIGCRICPI
                     PMPFEAGPDVARGQPILVIENNDTWASFAAWNRSAGCYSAIAYAGGGHGKSPAYDEAF
                     LDELRSRFDANALFYFGDLDPAGIRIASRAAQRRAQRGGVPLQPASALYDWLLTSGVR
                     APLPNSDQVLPSDLAWLPGVLREPVSVLFAAGQRIPQESLGTRALAGGGVGNPLYDHT
                     SRP"
     gene            2061..2420
                     /locus_tag="VM910_15030"
     CDS             2061..2420
                     /locus_tag="VM910_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63879.1"
                     /translation="MLVDALGSIPPGLLYPHQADGVAFLISKGRVILGDRARKVSGQT
                     PIVAWAAGVQAVSAAANAISARRLEQVGLKEIKLPALHRQRGTAGSGSVPRRRWYGDQ
                     RGSTPASEEVVLIYRKV"
     gene            2530..2886
                     /locus_tag="VM910_15035"
     CDS             2530..2886
                     /locus_tag="VM910_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63880.1"
                     /translation="MSGRETGPLEQYYDQKMLGMFTEDERSQLREFYCQLGNKFDEIV
                     SFLKERLGCSETDAIEEAEQAIDRWNEDVETFGTGRASSPPTPIQILLQDHYAIKEII
                     LNIEDQAMGRNLGTED"
     gene            2952..3260
                     /locus_tag="VM910_15040"
     CDS             2952..3260
                     /locus_tag="VM910_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63881.1"
                     /translation="MPDELDADDAASHSSGHTWSVRTITIPWTNPVPDSLNGIIHVLA
                     HNTPIKVDGREALLITIAKARHWIEDLTHGHVASFAAIARCGKPPRRSWSTWSTIRSR
                     "
     gene            3230..3484
                     /locus_tag="VM910_15045"
                     /pseudo
     CDS             3230..3484
                     /locus_tag="VM910_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3574..3639)
                     /locus_tag="VM910_15050"
                     /pseudo
     CDS             complement(3574..3639)
                     /locus_tag="VM910_15050"
                     /inference="COORDINATES: protein motif:HMM:NF018287.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
     gene            complement(3993..>6688)
                     /locus_tag="VM910_15055"
     CDS             complement(3993..>6688)
                     /locus_tag="VM910_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI63882.1"
                     /translation="VKKFAEMIGKPLHEAQRIYAQYDQRLPFISRLDAACQNEARQHG
                     YTVLYDGARRHWNNWAPGGRWQKGAGPCEREEAIRRIKDPSHPWYRQRLGRASTHTAL
                     NAQIQGDAARHTKLWMRACGREGIVPLLQMHDALELSVTTREQGELVARLACEVVKLE
                     VPMRVKVKFGRSWGDAKHSWEEIHGIAPATVTTEAASIQPQNGCLGDTEVLADSDFRV
                     SELPSGSGTEHNPVRAEHDERVEVHICAQCHLNPPDGSERPSAYNDEWLHPRCEEAFI
                     RTRMAEEGLLWEAPTAAHVNVAALGEAPPPLLPPRQINGSAASTSENGFATDGSFGTA
                     DHHAHSSDGNVHGNSGPTRGRHTAQWFYPYLDWSNYLRVDRYDLPNGERKFYQHHWDG
                     TSWVLGVKGTYAERKIPYRLPELKAALQANPDVVVHICEGETDADAMARLGFVTTTNP
                     GGALSWTPELTSWLRTLGVRRAAIHEDNDKEAQGYKGPKRSALLTAELSGFIKLKIVR
                     YPDVPRGEDVRWWLEHGHTKEELEARIAAAESATPTLPFINFANWDHEPIPEYEWSVP
                     DRYPLRQTVLSTGEGAVGKSILKLQLAAAHTLGGDWLGVSPAPGPALFVDAEDDAQVL
                     HIRLAAILRHYGATFADAIKGGLHLISLVGHDAVLGAPERNGKIKPTALYKQLLEAAG
                     DIKPKMIAIASSADVFAGNEIDRSQVRQFISLLTRIAIVANGTVSLIAHPSLTGINTG
                     TGLSGNTAWHNSVRARDYMTSIKPENGEQPDTDLRQIIFKKNQYGPVAETIVLRYQGG
                     LFLPEAGVDIDQAARNAKADEVFLELLHRLRTENRYVSSSKSVAYAPAVFAKEDAAKK
                     AGLNSTDFAGAMVRLFAAKKVWNEPYGRPSRPSYRIAPTTL"
BASE COUNT         1237 a         1988 c         2056 g         1407 t
ORIGIN      
        1 gcattgtcga caagttcctt caggacgacg agcggccact gcgccgggcg atgcccggtc
       61 tgcgcgatca gttctttctc gctcgcaaag tcgagcaggc gagacgtacg gaacgtctct
      121 cgttgaagac gcggctgtcg cggcggcgaa tggccggtcg agcagttccg caggcatggc
      181 ctaacctatg aggcggcgcc gaagccgaag agcgatctgt atcgcgacct gcttcctttg
      241 gtgaattcga agctggtcga cctggtcgac catccgaagc tggtgaatca gctggccagc
      301 cttgagcgcc gcaccgcgcg tggcgggcgg gatagcatcg accatgcgcc cggcgcccac
      361 gacgacatcg ccaacgccgt tagcggggtt gtcggcgcca tctctggcgt cggcgtgttc
      421 gattttgaat tctggcacga cgatcgcacc tggagtgggg gcgcaccgtc cggcgacgac
      481 ttgctttggt tcctcaggcg ctgacgttca tacaagaggc aaaggtcgac tataaaactc
      541 gtaagcctgg tgggttcaat tggctaccgc atcggcgtca tccagcgcgg taaggaacgc
      601 atgagcccgt cgtggtcctt gatgttttcg ctctcgctac cggtacagac gacgccaatc
      661 gcccgaccat ccttgtctat gatcggtgag cccgacatac cccctaccat cttgaccccg
      721 gtgatccaca gcgggccgcc gaaattgtgc acacgacagg catgccatcg caggtcgagc
      781 gacaagagaa acgcccaatc gcgcagcggc gcgtctggcc tggcgtaaga gcgcgcgccg
      841 cccggtgatc cagggtttac cgcagtttgt tatcgccgtg cagggtggga ggcttttgag
      901 agaagggtaa ggtaaacgta cttgtagttt caaattgtac gccaactcgt gcatcggttt
      961 gacgtgtttg ccaccgaaag cgacgtattc gcaggtaata ctagcctgcg gagacattga
     1021 ctcatgcgcg catgctccat gtcagagcaa gactcccata tcccattgga ccgccggacg
     1081 tttttgcgag acatcctgct tccccgtggc gcccgtagtt gggaccgggc aggcggcgcg
     1141 cccttgcccg gatttatcac tctcgccatt ccgcggccgg cgcgggcgcc caccatcgcg
     1201 cctggttatg gatggcatcc gctgttgagt ttcgccgcga gcgagcgcaa ccggctgcgg
     1261 cttcaagcgg ccaggcgcgt caatgaatgg ctcaagaccg atcccgacct gacactgatc
     1321 gtaccgatca aggagcgctc tctggaaata ttcggcgatg aaaagcgctt ggacgaattg
     1381 cggggcggca cgacgatgct gttcgggcaa ttggcactcg gggcgattgg ttgccgaatt
     1441 tgcccgatac ccatgccgtt cgaagccggc cccgacgtcg cgcgtggaca gccgatcctt
     1501 gttatcgaga acaatgatac ctgggcgtcg tttgctgctt ggaatcgctc cgctgggtgc
     1561 tattccgcca tcgcctacgc cggtggcggc cacggcaaaa gccccgccta cgacgaggcg
     1621 ttcctcgacg agctgcgttc ccgtttcgat gccaacgcgc tgttctattt cggcgatctc
     1681 gatccggcgg gcattcggat tgcaagccgc gcggcacagc gccgggcgca gcgtggtggc
     1741 gtgccgcttc agccggcttc tgctctttac gattggctgc ttacctcggg cgtacgagcc
     1801 ccgctgccaa acagcgatca ggttctgccc agcgatctag cttggcttcc gggcgtgctt
     1861 cgggagccgg tgagtgtcct gtttgcggcc gggcagcgca ttccgcaaga atctctcggc
     1921 acacgcgctc tcgctggggg cggcgtcggc aatccgcttt atgaccatac ctcacggccc
     1981 tgaccctccc ccatcttcta tgactcagct cgccccggac tggatttcgt tcgaagccga
     2041 caagacggag ggggcttgat ttgctggtcg acgccctcgg ctccattcca cctggcctgc
     2101 tctatccgca tcaggcggat ggggttgctt ttctaatctc taaagggcga gttattcttg
     2161 gcgaccgggc gcgaaaggtg tcaggccaga cgccgatcgt tgcgtgggct gccggcgtcc
     2221 aggctgtaag cgccgctgcg aacgccatca gcgcacgccg gttggagcaa gtgggattga
     2281 aagaaataaa attacccgcc ttgcacaggc agcgcggaac ggccggctct ggcagcgtgc
     2341 cccgccgccg ctggtacggc gatcaacgcg gtagcactcc tgcgtcggag gaagtcgtgc
     2401 tgatctaccg gaaggtctag gagggacttg tggctgcggg tcgaaaatcg cacggcaacg
     2461 tcaggttgga acaagacata ccgcaggttg actacgagca acagctaaag agcaacggag
     2521 agccttctca tgtcgggacg cgagacgggt cctttggaac aatactatga tcaaaaaatg
     2581 ctgggcatgt tcacagagga tgagcgcagc caattacggg agttttactg tcagcttggc
     2641 aacaagttcg acgaaatagt ctccttcctc aaagaaaggc ttggatgcag cgaaaccgat
     2701 gctattgaag aagcggagca ggcgatcgac cgatggaatg aggatgtcga gactttcggg
     2761 acgggccgtg cctcgtcgcc ccctactcca attcagattt tgctgcagga tcactatgcg
     2821 atcaaagaga tcatcttaaa tattgaagat caagccatgg gtcgcaatct tgggacggag
     2881 gattgagtcg cgagggtgcg cgcccgaccg actcccaaca ctgccgcgcg cagttcgaga
     2941 gcgagggcgc aatgccggac gagcttgatg ccgatgacgc tgcaagccac tcatcagggc
     3001 atacttggag cgtcaggacg attacgatcc cctggaccaa cccggtgccg gacagcctaa
     3061 atggcatcat ccatgtgctc gctcataaca cgccgatcaa agtcgacggc cgcgaggccc
     3121 tgctgattac cattgccaag gcccgccact ggatcgagga tttgacgcat ggccacgtgg
     3181 cgagttttgc cgcgatcgct cgctgcggaa agccaccgcg tcgaagctgg tcgacctggt
     3241 cgaccatccg aagcaggtga atcagctggc cagccttgag cgccgcaccg cgcggggcgg
     3301 gcgggatagc atcgaccatg cgcccggcgc ccacgacgac atcgccaacg ccgttagcgg
     3361 cgttgtcggc gccatctctg ccgtcggtaa atttgatttt gagttctggc tcgacgatcg
     3421 cgactggaat ggtggcgcac catcaagcga cgacttgctt tggttcctcc gtgctggagg
     3481 atgaacgaca cttcgcgcga acgcacctcc cgcaccgcgc gcgcgccgcg cccgccgtgc
     3541 gtgcagcacc actggaaacg tgcgcgggct ctcgtcggcg atcatgcgga tgaaaacatc
     3601 gagccggccg gtcgagcttt tgggattgac ggcggcgatc gctcaatccg atgtcgtgca
     3661 gcttagcggc gcggacgatg tgacactcag tgcccggtgc tgtagcaagt ctgggtcatc
     3721 aactgctcga cctcgcgctc ggtgagatac ttccggccct tgctcattga aacttctcgc
     3781 cggtggcaat ttttttcggc gcgagcggat gcgctgccac ccagcgtgca accggtgtgt
     3841 aaccggcgtg caaccgcgtg caaccgggtc tgcgcgcgtg gcgtggccaa ccgtgcaacc
     3901 catatatgtt gcacggttgg ccacggggcc tctccgtggt ttggttgcac gtttggttgc
     3961 actgcggccc ccgcgcagtg catggcgccg tctcacaatg ttgttggcgc gatgcggtag
     4021 cttggccgtg acggccggcc ataaggctcg ttccatacct tctttgcggc gaacagtcgg
     4081 accatggcgc cggcgaaatc ggtgctgttg aggccagcct ttttggccgc gtcctcttta
     4141 gcgaagacgg ctggcgcata tgccacgctc ttgctgctgc tcacgtaccg attttcggtg
     4201 cgcaagcggt ggagcaattc gagaaagacc tcgtcggcct ttgcgttgcg cgctgcctga
     4261 tcaatatcaa cacctgcttc gggcaggaac aatccgccct ggtaacgcag tacgatagtt
     4321 tcagcgacgg ggccgtattg gttcttcttg aagatgatct ggcgaaggtc cgtgtccggc
     4381 tgctccccgt tttccggctt gatactcgtc atgtagtcgc gcgcacggac ggaattgtgc
     4441 caggcggtgt tcccggacag gccggtacca gtgttgatgc cagtaaggct gggatgggca
     4501 atgagagaga ccgtgccgtt tgcaactatc gcaattctag taagcaggct gatgaattgg
     4561 cgcacttgcg agcgatcaat ttcattgccg gcgaatacgt cggccgatga tgcaatcgca
     4621 atcatcttcg gcttgatgtc gccggcggct tcgagcaact gtttgtagag agcagtgggc
     4681 ttaattttac cattgcgctc aggtgcacca agcacggcgt cgtgaccgac caatgatatc
     4741 agatgcagcc cgcctttgat tgcgtcggca aacgtcgccc cataatgtcg caagattgcg
     4801 gcgagacgga tgtgcagcac ttgggcgtca tcttccgcgt caacaaagag cgccggcccg
     4861 ggtgctggcg agacgcccag ccaatcgccg ccgagcgtgt gcgcggcagc gagttgcagc
     4921 ttcaagatgc tttttccaac ggcgccttcg ccggtggaga gaactgtctg gcgcagcggg
     4981 tagcggtctg gaacagacca ttcgtattcc gggataggct cgtggtccca attggcgaag
     5041 ttgataaatg gtagtgtggg cgtggcggac tcggccgctg cgatgcgcgc ttccagctct
     5101 tctttggtgt ggccgtgttc gagccaccac cgtacgtcct cacctctggg tacgtccggg
     5161 taacgaacga tcttcagctt gatgaagccg gacagttctg cggtcaataa agcactgcgt
     5221 ttgggaccct tgtagccctg cgcctctttg tcgttgtctt cgtgaatggc cgcgcggcgt
     5281 acgccaagag tgcgcagcca ggaggtcagc tcgggcgtcc aggacagggc accgccggga
     5341 tttgtggtcg tgacaaaccc tagccgagcc atcgcatcgg cgtcggtttc gccctcgcaa
     5401 atatggacca caacgtcagg gtttgcttgc agtgctgcct tcaattcagg gagccggtag
     5461 gggattttac gttcagcata tgtgcccttc acgccgagca cccaggatgt gccatcccag
     5521 tggtgttgat aaaatttgcg ttcaccattt ggcaggtcgt atcgatcgac gcgcagataa
     5581 ttggaccaat cgaggtacgg gtaaaaccat tgtgctgtgt ggcgaccgcg tgtcgggccg
     5641 ctattgccgt gcacgttgcc atcagaacta tgtgcatgat gatcagctgt gccaaaactg
     5701 ccgtcggtgg caaagccgtt ctcactcgtc gatgctgcgc tgccgttaat ttgccgcggg
     5761 ggtaatagcg gcggcggtgc ttctcccaat gcggcgacgt tgacgtgtgc agcggtcggc
     5821 gcctcccaca gcaaaccctc ttctgccatg cgggtgcgaa tgaacgcctc ttcgcaacgc
     5881 gggtgtaacc actcgtcgtt ataggcactc ggacgttcgc tgccgtccgg tggattgaga
     5941 tggcactgcg cgcagatgtg aacctcgact cgttcgtcgt gctcggcgcg aaccgggtta
     6001 tgctcggtcc ccgacccgct agggagctcg ctgacgcgaa aatcggagtc ggccaggacc
     6061 tcagtatctc ccaaacaacc gttttgcggt tgaattgacg cagcttcggt tgtgactgtg
     6121 gccggcgcga ttccgtgtat ttcctcccag gaatgtttgg cgtcgcccca gctgcggcca
     6181 aacttgacct tgacgcgcat cggcacttca agcttgacga cctcgcaggc cagccgcgct
     6241 acgagctcgc cctgctcacg cgtcgtgact gagagttcga gcgcgtcgtg catctgcagc
     6301 agcggcacga tgccctcacg cccgcacgcc cgcatccata gcttggtgtg ccgcgcagcg
     6361 tcgccttgaa tctgagcgtt gagcgcagtg tgggtgctgg cgcgccccag ccgttggcga
     6421 taccaagggt gactgggatc tttgatgcgg cgaatcgcct cctcgcgttc gcacggacca
     6481 gcgcctttct gccagcgccc gcccggcgcc caattgttcc agtgccgacg cgcaccatcg
     6541 tacaacaccg tgtagccgtg ctgtcgcgcc tcgttctgac atgcggcgtc caatcgcgat
     6601 atgaatggta gtcgttgatc gtattgcgcg taaatccgtt gtgcttcatg caacggcttg
     6661 ccgatcatct cggcaaactt cttcaccc
//
LOCUS       DATLJT010000649         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4175022,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000649 DATLJT010000000
VERSION     DATLJT010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175022"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VM910_15060"
     CDS             <1..632
                     /locus_tag="VM910_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63883.1"
                     /translation="NIDPVASGLVASLNRPGGNLTGVSYLTSELGAKRLGLMHEMVPR
                     VTDFAVLAHPTYPTSAPFISDVEAAARSLGFRIEVFNASTESEIDTAFAGVSARGLGA
                     LLIANNTLFTSRREHISALAARYALPTMYAWREFANAGGLICYGPSFPDVYRLIGGYA
                     GRILKGEKPADLPVMQPTKFELVINLKTAKALGLEVPDRLLALADEVIE"
     gene            629..1618
                     /locus_tag="VM910_15065"
     CDS             629..1618
                     /locus_tag="VM910_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63884.1"
                     /translation="MRRREFITLLGSAALAPLVNPYPACAQQRGKVPTIGFLGATTPS
                     IWSAFIPPFQQRLRELGWIDGQNIAIEYRWAEGREDRYAEFADEFVRRKVDVIVTAST
                     AAAAAAKSATTTIPIVFAAAGDPVGTGLVASLARPGGNVTGLSNQQTDLAGKRLEQLH
                     RVVPGLKRLAVMGNIHTLNVALEMNEVQSVGPKFGIEVTRLEITKQEDIVPGLEALKD
                     RVDGLYVCTDPLLTTYRTLINTFALREKLPTMYAFREYVLSGGLMSYGPNFPDLFRRA
                     GDYVDKILRGVKPADIPIEQPVKFDLIINLKTAKTLGLTVPDKLLATADEVIE"
     gene            1910..2113
                     /locus_tag="VM910_15070"
                     /pseudo
     CDS             1910..2113
                     /locus_tag="VM910_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2387..>2646
                     /locus_tag="VM910_15075"
     CDS             2387..>2646
                     /locus_tag="VM910_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63885.1"
                     /translation="MKMVLSAIAVSAAVVATDASAQGVNLTGPYRCVAGCVSAQPGLA
                     FVTQNGWELNLVNEVGEPSRGWVDYPGRLWIARANLGAIYSP"
BASE COUNT          538 a          780 c          820 g          508 t
ORIGIN      
        1 tcaacatcga tcccgtggca tccgggctgg tcgcgagcct caaccgcccg ggcggcaatc
       61 tcaccggtgt gagctacttg acctccgagc ttggagcaaa gcgactgggg ctgatgcacg
      121 agatggtgcc aagggttacc gacttcgccg tcctcgcgca cccgacctat ccgacctccg
      181 cgccgttcat cagcgatgtt gaggcggccg ctcgcagctt gggctttcga atcgaggtgt
      241 tcaacgcctc gaccgaaagc gaaatagaca ccgctttcgc gggtgtgagc gcgcgcgggc
      301 tgggcgcgct ccttattgcg aataacacgc tgttcacgag ccgccgggag cacatcagcg
      361 cactcgctgc gcgctacgcg ctgccgacaa tgtacgcgtg gcgggaattt gcaaacgctg
      421 gcggcctcat ctgctatgga ccgagcttcc ccgacgtgta tcgcctaatt ggaggttatg
      481 caggtcgaat cctaaagggt gagaaacccg ccgacctgcc ggtcatgcag cccacaaagt
      541 tcgagttggt gattaacctt aagacggcaa aggcgcttgg acttgaagtt ccagataggt
      601 tgctcgcgct cgccgacgag gtgatcgaat gaggcgccgc gagttcatca cgctgctcgg
      661 cagcgcggcg ctagcgcccc tggtcaatcc gtaccccgca tgcgcacaac agcggggcaa
      721 ggtgccgact attggttttt tgggcgcgac cacgccttca atctggagcg cgttcatccc
      781 gccttttcag cagagactgc gtgagctcgg ctggatagac ggtcagaaca tcgccatcga
      841 gtatcgctgg gcggaaggac gcgaggaccg ctacgctgaa tttgcagatg agtttgtacg
      901 ccgcaaggtc gatgtcatcg tgacggcgag tactgcggcg gccgcagccg cgaagagtgc
      961 aaccacgaca atcccgattg tctttgccgc ggcgggggac ccggtcggca ccgggctggt
     1021 cgccagcttg gcgcgcccgg gcggcaatgt gacaggcctg tcgaatcagc agaccgatct
     1081 tgcgggcaag cggctcgagc agttgcacag agttgtcccc ggtctcaagc ggttagccgt
     1141 tatgggcaat atccacacac ttaacgtcgc gttggagatg aatgaagtgc agtcggtagg
     1201 acccaagttc ggcatcgaag tcaccagact cgaaatcaca aaacaggagg atatcgttcc
     1261 cggcctcgaa gcactcaagg accgcgtgga tgggctttat gtttgtactg atccgctcct
     1321 gacaacgtat cgaactctca tcaatacctt cgcgctcagg gagaaactgc cgacgatgta
     1381 tgcgtttcgc gagtacgttc tgtcgggagg tctgatgtct tacggaccga acttcccgga
     1441 ccttttccgg cgtgccgggg actatgtcga caagattcta cgcggagtga aaccggcgga
     1501 cattccgata gagcagccgg tcaaattcga tctcattata aatctcaaga cggcgaagac
     1561 gctcggcctc accgtgccgg acaagttgct cgccaccgcc gacgaggtga tcgaatagtc
     1621 tggatcggtg gagttcatca cccactgcca ttgacccacc tagctgctaa gtgtgaggcc
     1681 ctggggcaac ctggggcctc actgctattg gggcaaagtg ttcagcactg cttgaaagtg
     1741 gttacacgaa tcacatttcc gtgatcggat cttgcggcta agagggaacc ccatgttcca
     1801 gggtcgcggc ttcggcaatt ccgccgccgc gaattctagg agccttgaac atggcaaccc
     1861 gaatcactac ctatccgaac acacagcgga gagacaacgg acccactgcc atcggccgaa
     1921 cgagcgcaac aaggtgcagg ccttcaacga ttttctcgtc ttcggctcga tggcgatcgg
     1981 ctcattctcc tccgggacgc tgttggtgag cttcggatgg agcatggtga acggggtcgt
     2041 ggtcccggtg gcgctggcgg caactgcgct gctcgtctgg ggcaggctgc gggggcgcag
     2101 gcagagcgcc tgatgccggc gacgacggta tcattcgacc gaagatcgat gggctgattc
     2161 cctggtgctc gcgagcatga cagtgaccga cgtcgcgatg cgcagcatgc ccgccgcgcc
     2221 gaccagccgt taagcaaacg aatcactggg cccacgagcc gccttgctgc tgaaatcgaa
     2281 aagctacatc ttgagccgag aggtgaaggc gcgcgcaagc gctgcaaaaa agcggcgccg
     2341 gcaaccttcg agccagcctg aggattcact gaccgaggag cacgcaatga aaatggtact
     2401 gagtgcgatt gccgtgtcgg cagcggtggt cgcaaccgac gcgtcggccc aaggcgtgaa
     2461 tctgacgggc ccatatcggt gcgtcgcggg ttgcgtatcg gctcaaccgg gcctcgcctt
     2521 cgtcacccag aacggttggg agctcaacct ggtcaacgag gtcggcgagc catcccgagg
     2581 ctgggtggat tacccgggtc gcctgtggat tgcccgggcc aatctcggcg cgatctattc
     2641 cccgga
//
LOCUS       DATLJT010000650         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3255670,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000650 DATLJT010000000
VERSION     DATLJT010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255670"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1180)
                     /locus_tag="VM910_15080"
     CDS             complement(269..1180)
                     /locus_tag="VM910_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI63886.1"
                     /translation="MILAALNAAGKNIDRLEPDDLAPIDELHSRGRESTSDLARLLAL
                     KGDETVLDVGCGIGGPSRYLAKTYGCRVTGLDLTPEFCQVAAMLAERTGLAGKVEYRQ
                     GDALAMPFADRSFDVVWSQSVAMNIADRHRLNNEVKRVLKPTGRFAFSDVVDGGGGPP
                     HFPVPWARECSISFLLTAQARRAKLEAAGFDLITFEDQTARALARAEGRMKPDGAPLA
                     LGLHIVLGQDAPTMLKNMLRNYQEGRVGLVQGIATRRNKNNVSPRNRAPSQRRSASMS
                     PDRTRARRRSDRAGSQICCTALCHFWH"
     gene            complement(1372..1791)
                     /locus_tag="VM910_15085"
     CDS             complement(1372..1791)
                     /locus_tag="VM910_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI63887.1"
                     /translation="MKVKDAMHKGVDWVSPETPVTELAKLMHEHDIGAIPIGENDRLI
                     GMVTDRDIVCKGLAQDSFDAGRATARDVMTPGIHCCRDDDDLAKAVRHMEKLQVRRLP
                     VINKGRRMVGILTLGDVSNSAPGDLLSECFKSVSAHH"
     gene            2107..>2324
                     /locus_tag="VM910_15090"
     CDS             2107..>2324
                     /locus_tag="VM910_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63888.1"
                     /translation="MATVGGGAYTYEPVENWAKLPPGWSFKEIGGVGVDRHDNVFVFN
                     RGEHPMIVFDRDGNFLRSFGEGMFPRAHG"
BASE COUNT          454 a          715 c          677 g          478 t
ORIGIN      
        1 aacgccctta tcgacgcgct atggcgaaat ccgggagatc ccgcccgaat gctcgtccgg
       61 ctatggctgg ggtcagccac cccgagcggg gtcaggtcgt gccgtcgcca gaaacacgac
      121 gcctttcatg gtcctcctcc ggtgcgcgct gttgcgtggg cgcctgacga gggcgcgtac
      181 tcataaatca tgattgggtt ggcaatcgcg gctgatgtcc gctttacccc cgaaagcgga
      241 catcgagcga ggcgatgggc atgtccgctt agtgccaaaa gtgacataac gcggtgcagc
      301 aaatttgcga gcctgctcga tcacttcgtc ggcgcgcgcg agtacggtcg ggggacatcg
      361 aggccgagcg cctttgcgaa ggggcgcggt tgcgcggact aacattgttt ttattccgac
      421 gggtagcgat gccctgaaca agaccgacac gtccctcctg ataattgcgg agcatattct
      481 tcaacatcgt tggcgcgtcc tgccccagca cgatgtgcag accgagagct aacggcgcgc
      541 cgtcaggctt cattcgtccc tccgcccggg ccagcgcgcg agccgtctga tcttcgaaag
      601 taatgaggtc gaaacccgca gcctcaagct ttgcacgcct ggcttgagcg gtgagaagaa
      661 agctgatcga gcattcgcgc gcccaaggaa ccggaaagtg cggtggcccc ccgccgccat
      721 cgaccacgtc tgagaacgcg aagcggccag tgggtttcaa cacgcgctta acttcgttat
      781 tcaaccggtg gcgatcggcg atgttcatcg ccacgctctg tgaccaaacg acgtcgaagc
      841 tgcggtccgc gaatggcatg gcgagggcgt cgccttggcg atattcgacc ttgccggcaa
      901 gtccggtgcg ttcggccagc atcgcagcaa cttggcaaaa ctctggcgtc agatcgagcc
      961 ctgtgacacg gcagccgtag gttttggcga ggtaccgcga tggcccgcca ataccacagc
     1021 ccacgtcaag taccgtctca tcgcccttga gagcgagcag ccgtgcaaga tcgctcgttg
     1081 actctcgtcc gcgtgaatgg agttcgtcta tcggtgcgag atcatcaggc tcgagacgat
     1141 caatattctt gcccgcagcg ttgagggcag cgaggatcac ggtgccgaga tcggcacgcc
     1201 cgtagtgttc ggcaattgtt tgggtgctag ccattgggga agggacactc catgcgttcg
     1261 atgtttcaag ctgccaacgg aagcttgtcg aacactactc gatgccaagt ttgcacagca
     1321 ccggcccggc gagcataggt tcccgccggg cgaatagcgt cacacttgtt ttcagtggtg
     1381 agccgaaacg cttttaaagc attcggacag cagatcaccg ggtgccgaat tgctgacgtc
     1441 gcccagagtg aggatgccga ccatccgcct gcccttgttg atcacaggca gcctgcgcac
     1501 ctgcagcttc tccatgtgcc gcactgcctt ggcgaggtca tcatcatctc gacagcaatg
     1561 gatgcctggg gtcattacat cgcgcgcagt cgcgcggccg gcatcgaagc tgtcctgcgc
     1621 caatcctttg cagacgatgt ctcggtcggt caccatgccg atcaaccggt cgttctctcc
     1681 aatcggaatg gcgcctatgt catgctcatg catcaatttc gccagctcgg tgacaggcgt
     1741 ctctggactg acccagtcga cacccttgtg cattgcgtcc tttactttca cggcggacct
     1801 ccgttgctga agtttgcgta tcagccccgg ttccctcggc tctccgacta tacaacggat
     1861 cggatccttt cgcacggcct caaggccacc gcactcacat attagctcgg aggttgttcc
     1921 gagaccgcaa acgtccgctt tgggtcacaa gcagacctca cgtcatcact tgggcaaagt
     1981 ccgcttcgcc cctcaaagcg gacatggcgc gtttgcctca acatgtccgt tcagttcgga
     2041 actggctggc tcaacctttt gcttcgtgac aagcttccga ccggcaacga acaggggagc
     2101 gcgggcatgg ctaccgtcgg cggcggcgca tacacctacg aaccagtgga gaactgggca
     2161 aaactcccgc ccggctggtc attcaaggaa atcggcggcg tcggcgtaga tcggcacgac
     2221 aacgtctttg tcttcaatcg cggcgagcac ccaatgatcg tgttcgaccg cgacggcaac
     2281 ttcctgcgct cattcggaga aggtatgttt ccccgtgccc acgg
//
LOCUS       DATLJT010000651         2574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3026275,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000651 DATLJT010000000
VERSION     DATLJT010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3026275"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..859)
                     /locus_tag="VM910_15095"
     CDS             complement(20..859)
                     /locus_tag="VM910_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI63889.1"
                     /translation="MVRRGGSGPPLLLLHGNPQTHAMWHAVAPILAQHFSVVATDLRG
                     YGDSSKPPTDARHAPYSKRTLAADQLKVMQRLGYEGFFVAGHDRGGRVAYRMALDAPT
                     AVRKLAVLDIIPMGEAFARMDHKVALGYWQWLFMAQPYDLPEHFIGLDPEGFLRRLLA
                     RGPQALTFFTPAALDEYIRCFSNPDTLHAICEELRAGATIDCEHDWADQRDGRKIACP
                     VLVLWGSRGRLSEWYDVLAVWRGWAEHVSGRSLATGHYLAEEAPSETAQELISFFTAG
                     AAG"
     gene            complement(1374..2429)
                     /locus_tag="VM910_15100"
     CDS             complement(1374..2429)
                     /locus_tag="VM910_15100"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HVI63890.1"
                     /translation="MRLRLEHGRRDDLKTLHPAYFALVMATGIVSVATYLHGIPVLPK
                     VLFWLNTLFLAGLLTATGARILQYPRAFTADIQNHSRGVGFFTTVAALAVFGTELVLL
                     MDAAGIAAMFWIAAAVLWFVTIYGVLAVLTVKPDKPRLADGLNGGWLVSAVAFQAVAM
                     LTVLISAAGVLAGFERPLLFAALVLWLGGGALYLWIMTLIFFRYTFVQMAPEDLTPPY
                     WINMGAVAISALVGATLLEHDALSRDVAEIVPFVKGFTLFYWAIATCWIPMLLVLGFW
                     RYLICGVPLAYDPLYWGGVFPLGMYSVSTYHLAKILAAPFLMPLSQLFMVVAVLAWAA
                     TFAGLVDSRLWRPRSPD"
BASE COUNT          561 a          811 c          782 g          420 t
ORIGIN      
        1 ctagcagccg gtgccggatt catcctgccg cgcctgccgt gaagaagctg atcaattcct
       61 gcgcggtttc cgacggagcc tcctcggcga ggtagtggcc ggttgcgagc gaccggccgc
      121 tcacatgctc cgcccaaccc cgccatacgg cgagaacgtc ataccattct gacaatcgcc
      181 cccggcttcc ccacaggacg agcaccggac aggcaatctt ccggccgtcg cgctgatcgg
      241 cccagtcatg ctcgcaatcg atcgtcgcac cggccctgag ctcctcgcag atcgcatgca
      301 gcgtgtccgg attggagaag catcggatgt attcgtcgag cgccgcgggc gtgaagaatg
      361 tcagtgcctg cggcccgcgc gcaagaaggc gccgcaggaa gccctcggga tcaagcccga
      421 tgaagtgctc gggcagatca tagggctgcg ccatgaacag ccattgccaa tatccgagcg
      481 ccaccttgtg gtccatgcgg gcaaaggcct cgcccatcgg aatgatgtcc agcacggcca
      541 gcttgcggac cgcggtgggc gcgtccaagg ccatgcgata ggccacgcga ccgccgcgat
      601 cgtgtccggc gacgaagaac ccttcgtatc caaggcgctg catgaccttg agctggtcgg
      661 cggcgagcgt ccgcttcgaa tagggcgcgt gccgcgcatc ggtgggcggc ttggagctat
      721 cgccgtagcc tcgaagatcg gtcgcgacta cggaaaaatg ctgcgcgagg atcggcgcga
      781 ccgcatgcca catcgcgtgg gtctgaggat tgccatgcaa cagcagcaac ggcggtccgc
      841 ttccgccgcg acgcaccatg atttcggctt ccccgacatt cagcctgtcc aaatcgaagc
      901 cctcaaacat tccccacccc ggttccagat tggccagtca acttccgcca gcgcagatcc
      961 tgattctgtc gcaacccccg gatcacgacg ccacggcgac ctaaccattt tgaggtcggg
     1021 ccaaatccga cagcatgcga gaggatattc gccatccgct cgattgcctc gtcgctgacg
     1081 gccgccgagc ctgacgcagg cgacggggcc cgaagcgcat cctaacgcca aactcagcgc
     1141 aaccccattc gcatgtccgc tcaaagcaga catcgaagcg cgactggatc atgtccgcat
     1201 tacccccgaa agcggacatg gaccaccacg gtcgtgatgt ccgcttagtg ccaatagcgg
     1261 acattggccc acttattcga tcacctcgtc ggcgcaagaa gaagatcatc accgatgttg
     1321 gccgcgatcg cgtgtgtcgc agccatgcaa ggtgtgcaga tattgcgtcc gccttagtct
     1381 ggcgatcgcg ggcgccacaa acggctgtcg acaagcccgg cgaaggtggc cgcccaggcg
     1441 agaacggcga cgaccatgaa cagctgcgac agcggcatca ggaacggcgc tgccaagatc
     1501 tttgccaggt ggtatgtgga gacggaatac atgcccaatg ggaatacgcc tccccaataa
     1561 agcgggtcat aggcaagcgg cacgccgcag atcagatagc gccagaagcc gagtaccagc
     1621 agcatgggga tccaacacgt ggcgatggcc cagtagaaca aggtgaagcc cttcacgaat
     1681 ggcacgatct cggcgacgtc gcgcgagagc gcatcgtgct cgagcagcgt ggcgccgacc
     1741 agggcggaga tcgccactgc gcccatattg atccaataag ggggcgttag atcctccggt
     1801 gccatttgca cgaacgtgta gcggaagaag attagcgtca tgatccacaa atacagcgca
     1861 cctccgccga gccataggac gagcgccgcg aacagtaacg ggcgctcaaa acccgcgagg
     1921 acgccagcgg ccgatatcag caccgtcagc atggcgaccg cctggaaggc aacagcgctg
     1981 accagccaac cgccattgag gccgtcggcc aaacggggct tgtccggctt gacggtgagc
     2041 acggcgagca cgccatagat cgtcacaaac caaagcaccg cagcagcgat ccaaaacatg
     2101 gctgcaatcc cagcggcgtc catcagcaac acaagctctg tgccaaatac ggcgagggcc
     2161 gccacggtgg tgaagaaacc cacaccgcgg ctatggtttt ggatgtcggc ggtgaacgca
     2221 cgagggtact gcaggatgcg cgcgcccgtc gccgtcaaca acccggcgag aaacaacgta
     2281 ttgagccaga acaggacctt cgggagcacg gggatgccgt gcaggtatgt ggcgactgag
     2341 acgataccgg tggccataac gagtgcgaaa taggccggat gcagcgtttt gaggtcgtcg
     2401 cgccgtccat gttcgagacg aagcctcata tcccagtgtc cccttggatg ttggcgcttg
     2461 gaatgcgcgg tccgatttcg cctattggaa gagattacag cagggatttg cggcagacga
     2521 aatggtttca gggttcagtt tgcgcggcaa tcctgagccg ctctgtccgc ttcg
//
LOCUS       DATLJT010000652        10351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_958004,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000652 DATLJT010000000
VERSION     DATLJT010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958004"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1330..1803)
                     /locus_tag="VM910_15105"
     CDS             complement(1330..1803)
                     /locus_tag="VM910_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63891.1"
                     /translation="MPARNSNLPAWVAFARGLDASRYCPVFVPDTNDTIEGSPKELRD
                     FVVFPEAAWNVALRMALYERAFINLGVNNGPMGLAWLNTRVCYATLKIETADVPQSSL
                     GFIRSFGFEPGKSLPFATPLQEWVWEDDTEEAITETFRRLSERIEASSESARPVG"
     gene            1963..2292
                     /locus_tag="VM910_15110"
     CDS             1963..2292
                     /locus_tag="VM910_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63892.1"
                     /translation="MLIVIALLIATAANAEPLPPQPQPKIGAVCPTGWSAIPTSGYCV
                     PGPNNKCRMMPKLGAPCLPGWSAYSTTASRTMPEAVRAGVSALGRGDHIHFWLPNQRL
                     DHGPDQR"
     gene            complement(2357..3025)
                     /locus_tag="VM910_15115"
     CDS             complement(2357..3025)
                     /locus_tag="VM910_15115"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HVI63893.1"
                     /translation="MRRCDFTAIAVAVAVGLSASCACAQEFTADLKGVNEIGSIPTAF
                     PAIPSTPHPSTPSGYTGAVLSNGTGTAKLVLNKNAGTVTYTLTYSNVGTTPPQTGMVS
                     QAHIHFGKSRDSGGILVFFCTNLNFAGTGPTPQACPTNSGTVSGTWTKDNVQAILGQN
                     VVAGDFNALVVALESNTAYANVHTIPLPGATNTAYPGGEIRGQVHEVEDQQGQDQNQQ
                     GNQN"
     gene            3506..3682
                     /locus_tag="VM910_15120"
     CDS             3506..3682
                     /locus_tag="VM910_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63894.1"
                     /translation="MQQQVDALDEPVAERPMLPEPDISRIPFRPATPMEQKASRGDLS
                     WDEQMRLFKERALA"
     gene            complement(3977..4177)
                     /locus_tag="VM910_15125"
     CDS             complement(3977..4177)
                     /locus_tag="VM910_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63895.1"
                     /translation="MRIIAPTFSVNLKVRAGEIVEADRPIRYMKGWTLARVLALAKRW
                     KWEVVFNDQERAELESGPPSAA"
     gene            complement(4178..4780)
                     /locus_tag="VM910_15130"
     CDS             complement(4178..4780)
                     /locus_tag="VM910_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63896.1"
                     /translation="MSIISTVWAGSARPPRSAYARKWLAARLVRDEQRKRPPITLPGR
                     PVVTKPTEPSPPSPPTTAPRTGWLKLPEGGAPAGYREMDPVDVLPPYTREQLVRMNDR
                     FVHRLERAFARGRESRTSAASFQPEKRYGGYGMPLALTDDQLATLRQFATPIPPRLRD
                     AYLRSVARRLAGKTIGDGTVHTACIGAQHEVINGARAGAA"
     gene            complement(4881..5084)
                     /locus_tag="VM910_15135"
     CDS             complement(4881..5084)
                     /locus_tag="VM910_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63897.1"
                     /translation="MMGEISEMKARAKANARRERQLRFHDIKIEACMSIDRLNRQQRW
                     RGIKAVSRSLLGGGLLYRQTVNR"
     gene            complement(5375..6922)
                     /locus_tag="VM910_15140"
     CDS             complement(5375..6922)
                     /locus_tag="VM910_15140"
                     /inference="COORDINATES: protein motif:HMM:NF042863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HVI63898.1"
                     /translation="MSRRKPATSDGPITAADVIEFIETVCLIPEGKLVGKPLVLQPFQ
                     KEIIELIYDNPRGTRRAIISVARKNAKTCLAACLLLAHLCGPPAHSRPNSQLFSAAQS
                     RDQAAIIFNLAAKMVRLNPALAKIVIIRETAKELICPELGTRYRALSAEASTAYGLSP
                     SFIVHDELGQVRGPRSPLYEALETATGAQENPLSIIISTQSPTDSDLLSVLIDDALAG
                     HDPHTVIKLYTAPRELDPFEEATIGLANPAFGTFLNPAEVLAMAAAAKRMPARQAEYE
                     NLILNRRVESSHAFIAPEVWKACGDPPGAMDGLQLYGGLDLSMVSDLTALVLIGWRED
                     KWRVWPTFWLPEEGLVEKGMADRVPYDLWRSQRHLAVTPGKTVSYEFVANHLRVLFAR
                     YNIAKLAFDRWNMQHLQPWLFKAGMSEQFVKEHFVEFGQGYASLSPAMRDLEQAILDG
                     KLAHGDHPVLNFCLNNTVVVLDDAGNRKPSKKKSTGRIDGTVALIMALGVAAKPAPQI
                     DVRSLIG"
     gene            complement(6894..7337)
                     /locus_tag="VM910_15145"
     CDS             complement(6894..7337)
                     /locus_tag="VM910_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63899.1"
                     /translation="MPRKSAASLAVVPQLPGTGRPEPPADLDDGEKRLWREVVNSLPP
                     HWIDAAGRIILRRLVAQAALCLSMEEQLRAHRAAGTVVETYAKLAKQHSDTAKQTESL
                     LASLRATPRSRSTSHAAGLQTRKTAEMRPWEIRSGRRVETQTGDE"
     gene            7382..7843
                     /locus_tag="VM910_15150"
     CDS             7382..7843
                     /locus_tag="VM910_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVI63900.1"
                     /translation="MDNPIPDRLSLRPVRTWYTLNRWRKIAKWQLRNEPLCRLCQNRG
                     LVRPATIADHIEPHRGDINKFWFGKLQSLCFDCHNTVKQGIEQRGFDTAVDADGWAID
                     PKHPVHTGAFVPDAKGDTHVEDRDSARRPNPASGRGADLSPGPDRRGPQIF"
     gene            complement(7976..8641)
                     /locus_tag="VM910_15155"
     CDS             complement(7976..8641)
                     /locus_tag="VM910_15155"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HVI63901.1"
                     /translation="MDKRHRRSYEREVEQPIAITPVEYSGLQEAYDHFNGTLFDGKLV
                     DVFITYQRHAHSRGHFAPDRFSGRTAVGGRHELALNPDHFVGRSDEEITSTLVHEMVH
                     VWQQQHGKPASRGYHNKQWAVKMKEVGLQPSNTGGAGGKETGQQMSHYVIPGGCFAGA
                     FAELAAMGWRLNLESAHRAGPDGGRKSKTKFTCFGCGQNAWGKPDLAVTCTPCGIEMR
                     LVD"
     gene            9074..9427
                     /locus_tag="VM910_15160"
     CDS             9074..9427
                     /locus_tag="VM910_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63902.1"
                     /translation="MPIVIEADGFEETAERVDKIILQLRELRGYEIGREFAEWQEQDV
                     NRKHAFVVKHRATWTTRFRPHSRWEMTRHRYAMRRLVRRGRYTGYYSTRPVLRQELLD
                     QLVTRMEALLNNIKW"
     gene            complement(9620..10003)
                     /locus_tag="VM910_15165"
     CDS             complement(9620..10003)
                     /locus_tag="VM910_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63903.1"
                     /translation="MGIDTSKGVLRIQDRCHGNSTAKDANPCDLAQTRAWVEDYKRWV
                     AGLPAVGDQAQPPDLQALVAHAGGYERITAEQWTEYDRAMARWQAARRPKDRRSEVTN
                     ASVANVVDARSADREDRSRNVAGRQ"
     gene            complement(10037..>10351)
                     /locus_tag="VM910_15170"
     CDS             complement(10037..>10351)
                     /locus_tag="VM910_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI63904.1"
                     /translation="LLPVSALVFVGDACEENGDSLVARAGGLGVPAFMFQEGDNETVA
                     TVFKAIALASHGAYGRFDAGSAKQLAELLKAVAAYATGGLAALEGRKDAGSIKLLGQL
                     KS"
BASE COUNT         2053 a         3210 c         3027 g         2061 t
ORIGIN      
        1 cgctcgcaat gcggacccca ctccgttcca atgggccaaa tccgccgacg acatcctggc
       61 ttctatcgag cgcttctgcc gccgaactct cgcaatccac gcagaagttg aataggaatt
      121 ttggaatcgg gacactagct cgtcgactat gcttttccgc cggcggcatc ggcgcggccc
      181 gcttgtagta atcgcccgca atggccgtga cgacatccgt cggaaccgat ggccatcgcg
      241 tccagtgtgg aacttcggca tcgaaagctt gtcgatgatt ttatcccttt catgtcagag
      301 agttctcggc gcggccgctg tcgccaccgt tacgcaaccc gtctgcgcag tagcaccgaa
      361 tttgatggca tcgtcccagt tgagtatagg ggcgagtctc aggtattacg attctcctgc
      421 cagaggcggg cgttgttgac cgaccagtat acggcagcaa cagtgagcag gccgacaagc
      481 tcttgcgtga cggaggtttg gatgcggaga gtaatatacg ctacttctgc tgctctcatt
      541 actctgggta caactcgctc tttggcggat cgttcgcttg cgcttcccct ctcggagcct
      601 cgagttaata ttgtggctat agctggaagt cccgtccaaa gggccgccta catttgtcgc
      661 cgctggtggc aatggcgcgg agctcgttgg gtccgaacct gttggcccgc tggttacgac
      721 ccatgctgga acgctcctgg ctgcagccct cctaccccgc agtacccgtt ctggcgcccc
      781 tggggatggc cgtacagtga ctggctcttc tactgagcat cgacgactga ttcgcgccga
      841 gacgacgagg gacaccggct ggccgattac ttcgacaggc ggagttcgtg gagtacctaa
      901 ccttcgacgc cgatgactgg gtgggcgcgg cgcagtggcg gggctctgcg aatatcccgg
      961 actgtctttt cagcgctctc cggtatgcgc ttgctcgttt gattctgtcc gacaagtgct
     1021 gacggtctac agtctgaatc gaggcccgaa tcgtccgctc gagccgaatc cgctcctcgt
     1081 tcactcgcgc gtgcttccag agtttgcgcg cagctttgtc aacaccggcg gtctgccacc
     1141 atgcgcgctc atcgccggcg cgatggactg cgcggtgatg ggagcagcac agcggtacaa
     1201 cgaattcgtc gctcgccttg cggccgagcg cccgcggctg caggtggcga agatgatgag
     1261 ggtccgacgg cttgcggccg ccctccacca ccttcgatgc gcctgagcgc ctcttccagc
     1321 gacgtcgggc taccccaccg gtctggccga ctcagagctg gcttcaattc gctcgctcaa
     1381 ccgccgaaaa gtctcggtga tggcctcttc ggtgtcatcc tcccataccc attcttgcag
     1441 cggtgtcgca aacggcagcg attttcccgg ttcgaaaccg aacgaacgaa tgaagccgag
     1501 agaggactgt ggcacgtcgg cggtctctat cttgagggtg gcgtagcaca cgcgcgtatt
     1561 gagccaggcg agacccatgg ggccattgtt gactccaaga tttatgaacg cacgctcgta
     1621 gagcgccatg cgcaacgcca cgttccacgc cgcctcggga aacaccacga aatcccgcag
     1681 ttcttttggc gacccttcga tggtgtcgtt ggtatctgga acgaacacgg gacaatatcg
     1741 gctcgcatcc aagccacggg cgaatgcaac ccaggccggg agattgctgt tgcgcgccgg
     1801 catgtaagag taccgccgaa gcgtgatcgc caccaagcga cgcgagcctg cgtgactctt
     1861 ggcccatgcg tcgaccgctc gcagttcttc cgcaggggca cgcaggcaag cgataccccc
     1921 ctgcccctgg gctgcgccgt ctcaagtaag ggaggcaatc acatgttgat cgtcatcgcc
     1981 ttgctgattg cgaccgctgc caatgccgag ccgctaccgc cgcaaccaca gccgaagatc
     2041 ggtgcggtat gcccgaccgg ctggagcgcc atcccgacga gcggctattg cgtgcccggg
     2101 ccgaacaaca aatgccggat gatgccgaag ctcggcgctc cctgcctgcc gggctggagc
     2161 gcgtattcga ctactgcgtc gagaacaatg ccagaggcgg tgagggcggg cgtcagcgcg
     2221 ctcggtcggg gcgaccacat acatttttgg ttacctaacc aaaggctaga ccatggccct
     2281 gaccaacgct gagaggcagg cccgctggcg agagcgccat gtcatcacct tgaccgacac
     2341 cgccgaggcc acgcaactag ttctggttgc cctgctggtt ctgatcctgg ccctgctggt
     2401 cttcaacctc gtgtacctgg ccgcgaattt cgccccccgg ataggccgtg ttcgtcgctc
     2461 ccggcagagg gatggtatgg acattcgcat aggccgtatt ggattcgagc gcaaccacca
     2521 gcgcgttgaa gtcgccggcc acaacattct gccccaggat cgcttggacg ttatccttgg
     2581 tccatgtccc ggagactgtt ccgctattcg tgggacaggc ctgtggagtc ggccccgtcc
     2641 cggcaaagtt cagattcgtg cagaagaaca caaggatgcc gccggaatcg cgggacttac
     2701 caaaatgaat gtgagcctgc gacaccatac ccgtttgcgg cggcgtggtt ccgacgttcg
     2761 agtaggtcag cgtgtaggtt accgtaccgg cgtttttatt cagaacaagc tttgccgtac
     2821 ctgtgccgtt ggacaatacc gctccggtgt agccgctggg tgtggagggg tgaggggtgg
     2881 acgggatcgc cggaaaagca gtcggtatcg atccgatttc gttgaccccc tttaaatccg
     2941 cggtaaactc ctgcgcgcag gcacaactcg ccgacaaacc aacagccacg gcgacggcga
     3001 tggcggtgaa atcgcatcga cgcatgatct cctcccttgt atcgaatcgt tgcgcgttct
     3061 cgcgcgcctc tcgattttaa cgaatactgc gggaggcaac cagagcaaag gttgatccaa
     3121 aagttggctg cattttcggc ccctcggcgg tggatttctg cggttcccga ttggcgaggc
     3181 aaaagggctt gataggaccg gactgcgcaa gaaagccaag ggatgaaggc catgtccatt
     3241 cgcatcggat atttgctgcc gacacgggaa ctatcgagga cgaacatgct ttgcgtttta
     3301 atccacaagc ttgggaaaaa tatctaaagg cacaacgccc tagtcaaaat atcgaagatc
     3361 gtcgcaagtt tgatccgacg aaggtgttga tggacacgtc tttacagcag cttgaggaat
     3421 aagagcgagg tttcccaatg agattgccca gattgttgcc gcttatggtg ttgatataac
     3481 tactgctgta ggaatttttc ttgctttgca gcagcaggtt gatgcgttgg atgagcctgt
     3541 tgctgaacgc ccgatgcttc ctgaacccga catttcacgt attccgttcc gcccggcaac
     3601 tcctatggag cagaaggctt cgcgagggga tttatcatgg gatgagcaga tgcgactgtt
     3661 caaggaacga gcgttggctt agtatgagac gggctattga ggacgaacat gtttttcgtt
     3721 ttaatccaga aaattgggaa aaatatctaa aggcgcagcc tcctagtcga catatcgaag
     3781 atagccgcaa gtttgatccg acgaaggtgt tgatggacac gtctttacag cagcttgagg
     3841 aataggggcg cccggcacaa aaaactcgca acccctatgt cccggaattc gtaaccagca
     3901 acagggagac gaggttcaga acttgtgtcg gttttggggt tagagcgtct cgcggccacc
     3961 gcgaggcgct tccgtatcac gccgcactcg gaggaccgct ctccagttca gcgcgctcct
     4021 gatcattaaa gacaacttcc catttccacc gcttcgccag cgcaagcacc cgcgccagcg
     4081 tccagccctt catgtatcga ataggtcgat ccgcctcgac gatctcgccg gcgcgaactt
     4141 tcagattgac gctgaacgtc ggagcgatga tgcgcattta cgccgccccc gcacgcgcac
     4201 cattaatcac ctcgtgctgg gccccgatac aggcggtgtg cacggtacca tcgccgatcg
     4261 tcttgccggc gagccggcgc gccacgctgc gcagatacgc atcgcgcaac cgcggtggaa
     4321 tcggcgtagc gaactggcgc aacgtcgcga gttgatcgtc ggtcaatgca agcggcatcc
     4381 catagcctcc atagcgtttc tccggttgaa aggacgcggc ggaagtgcgg ctctcacgcc
     4441 cgcgcgcaaa tgcgcgttca agacggtgaa cgaagcgatc gttcatgcgc acgagctgct
     4501 cgcgcgtgta cgggggtaaa acatcgacgg ggtccatctc gcgataacca gccggcgcgc
     4561 cgccctcggg cagcttgagc catccggtgc gaggggcggt cgtcggcggc gacggtggcg
     4621 acggctccgt aggcttggtg acgaccggcc gccccggcag cgtgatcggc ggccgcttgc
     4681 gctgctcgtc acgcaccagg cgcgcagcga gccacttccg cgcgtaagcg gaccgcgggg
     4741 ggcgcgccga accagcccat actgtcgaaa tgatagacaa gtggacgcct cccgattaca
     4801 agcggtggcg tctttttgtt tccgtcgttt tcctccgact aggccccggc ctcccatgca
     4861 ccggggctct tctttttctt ttaccgattt actgtttgcc gatacagcag accgccccca
     4921 agcagcgagc gcgacacggc ctttattccg cgccagcgct gctgccggtt gagccgatca
     4981 attgacatgc acgcttcaat tttgatgtca tgaaagcgca gttgccgctc gcgccgcgcg
     5041 ttagccttgg cgcgcgcctt catctcggaa atctcaccca tcattacttc tccttcgcgt
     5101 atacggggtg acacgggtcc aacggcatac cgtcgagacc gacatcgcaa cggaacccgt
     5161 ggtcagcgat catgcgttca gtaatgtcac gacactccct acataaggag atcaaaaact
     5221 cgccgcgata atgcccctcg gctggcacgg ccatcaccgc tcgtgtaacg attccgcgct
     5281 ccgcgcaata agcgcacaac gggtgagcac aaagctgctg aaccgaaacc cgtgtccaac
     5341 attcaatatc aaaacctgtc atgcgcacgc cccttcaccc aatcagactg cggacatcta
     5401 tctgcggcgc cggctttgcc gcgacaccca acgccatgat caacgcgacg gtgccatcaa
     5461 tacgtccggt agatttcttt tttgacggtt tacgattgcc cgcgtcatca agaaccacca
     5521 ccgtattatt gagacagaag ttcaaaactg gatgatcccc atgggcgagc ttaccgtcga
     5581 ggatcgcctg ctccagatcg cgcatcgccg gcgaaaggct ggcatatcct tgcccgaatt
     5641 ccacgaaatg ctctttcacg aattgctcac tcataccagc cttgaacagc cacggctgaa
     5701 ggtgctgcat gttccagcga tcgaatgcga gctttgcaat gttgtagcga gcgaacaaaa
     5761 cgcggagatg attggcaaca aactcgtaac tgacggtctt ccccggcgtc acggcaagat
     5821 gcctctgcga acgccacaag tcatagggaa cgcgatcggc cattcccttc tcgaccagcc
     5881 cctcctccgg caaccaaaac gtaggccaga cacgccactt gtcttcacgc catccgatca
     5941 ggaccagcgc ggtgagatcg ctgaccatgc tgagatcgag accgccatag agttgaagcc
     6001 cgtccatcgc ccccggcgga tcgccgcaag ccttccacac ctccggtgcg atgaacgcat
     6061 ggctcgattc gacgcgacga ttaagtatca ggttctcata ctcggcttgg cgggcgggca
     6121 ttcgctttgc ggccgctgcc atcgcgagga cttccgccgg atttaggaac gtcccgaaag
     6181 ccggattagc aagcccgatc gtcgcctcct cgaatggatc aagctcgcgc ggcgcggtgt
     6241 agagcttgat caccgtgtgc ggatcgtgcc ccgcgagcgc atcgtcgatc agcacagaga
     6301 gcagatccga atcggtcggg ctttgcgtgc taatgatgat gcttaacgga ttttcctgag
     6361 cgccagtcgc ggtctccaac gcctcgtaga gcggagagcg cgggcctcga acttgaccta
     6421 gctcatcatg cacgatgaag cttggtgaaa ggccatatgc tgtcgatgct tcggctgata
     6481 acgcacgata acgcgtaccc agctcgggac agatcaattc cttggcggtt tcgcgaatga
     6541 tgacgatctt cgccagcgcc ggattgagtc gcaccatttt ggcggcgaga ttgaagatga
     6601 tcgcggcctg atcgcgcgac tgcgccgcgg agaacaactg actgttggga cgactgtgcg
     6661 cgggcggccc gcagagatgc gcgagcagga gacacgcggc tagacaggtc ttcgcgttct
     6721 tgcgcgcgac ggaaatgatg gcgcggcggg tgccgcgcgg attgtcgtag atcaactcaa
     6781 tgatttcttt ttggaacggc tgcaacacaa gcggcttgcc gacgagcttc ccttccggga
     6841 tcagacaaac cgtctcgatg aactctatga catcagcggc agtgatcgga ccatcactcg
     6901 tcgccggttt gcgtctcgac acgacgaccg ctccggattt cccacggacg catctcagcg
     6961 gtttttctcg tctgtaatcc cgcggcatgc gaagtgctgc gcgaccgcgg cgtggcgcgc
     7021 agcgaggcga gcaggctctc ggtctgtttg gccgtatcgc tatgctgctt cgcaagcttg
     7081 gcatacgtct cgacgacggt gcccgccgcc cggtgcgcgc gcaattgctc ctccatgctc
     7141 aggcagagtg ccgcctgcgc gaccagacga cgaagaatga tccgtcccgc cgcgtcaatc
     7201 cagtgcggcg ggagagaatt gacgacctcg cgccagagtc tcttctcacc gtcgtcaaga
     7261 tctgcgggcg gctcggggcg tcctgttcca gggagctggg ggacgacagc gagcgaggcg
     7321 gcggatttgc ggggcatggt tcccgtatac gccagcacga agacgcggta aaccacattt
     7381 catggacaac cccattcccg accggctttc gctccgcccg gtgcgcacgt ggtacaccct
     7441 taaccgatgg cgcaagatcg ccaagtggca actccgcaac gagccactat gtcggctatg
     7501 ccagaaccgc ggcttggtcc ggccggcgac catcgccgat cacatcgagc cgcaccgcgg
     7561 cgacatcaac aagttctggt tcggcaaatt gcaaagcttg tgcttcgact gccacaacac
     7621 cgtgaagcag gggatcgaac aacgcggttt cgacacagcc gtcgacgcgg acggctgggc
     7681 catcgatccc aagcaccccg tccacaccgg cgcttttgtt cccgatgcga aaggtgacac
     7741 ccatgtcgaa gatcgagact ccgcccgccg ccccaacccc gcttccggac gcggagcgga
     7801 tctatctcca gggccagatc gacgaggtcc gcagatcttt tgaagccaag cttcggggcg
     7861 ttctgagcgc gccgatgctc cggatgacct gcggcaccgt cacgacgcaa ggggcgcgcc
     7921 tcggcgctgc cgtgccgcct gatgctttcc cactttttcg ttcgtacgac agaattcaat
     7981 ccaccagtcg catttcaatt ccgcacggcg tgcatgtcac cgccaaatcc ggcttgcccc
     8041 acgcgttctg cccacagcca aaacaagtga acttggtctt gctcttgcgg ccgccgtcag
     8101 ggccggcgcg gtgcgcggac tccaggttga gtctccaccc catcgcggca agctcagcaa
     8161 acgctccagc gaagcaaccg ccggggatca cgtaatgcga catttgctgg ccggtctcct
     8221 tgccgccggc gccacccgtg ttcgacggct ggagaccaac ctccttcatc ttcaccgccc
     8281 actgcttgtt gtgatagccg cgcgaggctg gcttgccgtg ctgttgctgc cagacgtgca
     8341 ccatctcgtg cacgagggtt gacgtgatct cctcgtcact gcggccgacg aagtgatcgg
     8401 gattgagcgc cagctcgtgt ctaccgccga cagcagtgcg ccccgagaac cgatcgggcg
     8461 cgaagtgccc gcgcgaatgc gcgtgccgct gataggtgat gaacacgtcg acgagcttgc
     8521 cgtcgaacag cgtcccgttg aagtggtcgt acgcctcctg caatcccgaa tactcgaccg
     8581 gcgtgatcgc gatcggctgt tcgacctctc gttcgtacga acgacgatga cgcttatcca
     8641 tggtcatgtt ctcccttcga ttacgcttct tccggtagtt gcacctcggc gctgccaagg
     8701 ggcttcggaa atggcccaga aagctagggt cgggacgggc ccgacggcgt aacgattttt
     8761 gggctaaggg ggggtcccca agtagcggta aacgttagca aaattcgggt cgcgggccgc
     8821 cgcggccatc gaccatccga aaattttcgt acctcccccc gccccgaaat cgcgccgccg
     8881 aatctatttg catccccgtc gcgccgcaca cgcgagcccc caggggcgat gatgcgccga
     8941 gcaggctcgc catcgcccac gccgactgcg cctgtattcg cggcgacgga ccgatcgcgg
     9001 cggtgactga tccttgtcga tcgacagcca acgctggata gtatcggatc gcgtgcacta
     9061 gcaaggatcg atcatgccga ttgtgatcga ggctgacggc tttgaagaga cagcagaacg
     9121 cgtcgacaag ataatattgc aactgcgcga gctacgcggt tacgagattg gtcgggaatt
     9181 tgcggaatgg caggaacagg acgtaaaccg aaagcatgcg tttgtcgtga agcatcgggc
     9241 gacgtggaca acgcggttcc gaccacatag tcggtgggaa atgacccgac accgatacgc
     9301 gatgcgacgg ttggttaggc gtgggagata caccggctat tactctacac gtccggtcct
     9361 gcgccaagaa ctgctcgacc aactcgtgac gcgcatggag gcgctgctca acaacatcaa
     9421 atggtgacgc attcagtgct tgagtcggcc cgcaagcgga ctccaagtca ccaggagttg
     9481 accgtcattg tacagcacct cgagcttgaa ccgccgaccg agcgctttga actctcgaac
     9541 cgatgacgga aacggcttct tcgtgcgata gagaagacct cgccgtggcg gtcccgggcc
     9601 agtgcaagcc cagactaatt cactgcctgc cggcaacgtt tcgagagcga tcttctcgat
     9661 cagcgcttcg ggcgtcaacg acgttcgcaa ccgacgcatt ggtcacctcg ctccgacgat
     9721 cctttggccg ccgagccgcc tgccaccgtg ccatcgctcg atcgtattcg gtccactgct
     9781 cggcggtgat gcgctcgtag ccgcccgcat gcgcgaccag cgcttgcaga tccggcggct
     9841 gagcctgatc gccaaccgcc gggaggcctg cgacccagcg cttgtagtct tcgacccagg
     9901 cgcgggtctg agcgagatcg catgggttgg catccttggc cgtcgagttg ccatggcatc
     9961 ggtcctggat ccggaggacg cctttcgatg tgtcgatccc catgctaacc taccgaagtg
    10021 cacatccagg cctagactag ctcttcaatt gcccaagcag tttgatggag ccggcatcct
    10081 tacggccctc gagcgcagcc aggccgcctg tggcgtaggc cgcgaccgcc ttgagcagtt
    10141 cggcgagctg cttggccgat ccggcatcaa agcgcccgta ggcgccgtgc gacgcgagtg
    10201 cgatggcttt gaacaccgtc gcgacggtct cgttgtcgcc ctcctggaac atgaaggcgg
    10261 ggacgccgag cccgccggcg cgagcgacga gggaatcacc gttctcctca caggcgtcgc
    10321 cgacgaagac cagggccgaa accggcaaga g
//
LOCUS       DATLJT010000653         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2797254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000653 DATLJT010000000
VERSION     DATLJT010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2797254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VM910_15175"
     CDS             complement(<1..636)
                     /locus_tag="VM910_15175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI63905.1"
                     /translation="MTRPFEGVKILDFTQVLAGPYASYQLALLGADVIKVERREGEDM
                     RRTPLSREWAERGLAPGWQAINGNKRSLTLDLAKPEAIAIVKKLAAQADVVMENFRPG
                     VMDKLGIGYAALSEINPRLIYCAVSGFGQTGPERLSPGYDGKIQAMSGIMAITGHEET
                     GPTRAGFAVCDVLSGATAAFGVSSALFQRNQTGKGQLVDVSMLEATLAFLSG"
     gene            complement(808..1737)
                     /locus_tag="VM910_15180"
     CDS             complement(808..1737)
                     /locus_tag="VM910_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HVI63906.1"
                     /translation="MSGPDDAQFGFAPDYDAPVPYMQRTRDYYIAIGYTTPYRWAHHV
                     DAPFQPLKKPLDQSRVTIVTTAAPYDLAKGDQGPGAAYNGGAKFYKVYDGDTSKEHDL
                     RISHIGYDRKHTSATDSGTWFPLPQLLKAQAKRRIGAVAPRFFGAPTNRSHRVTIDVD
                     APEILARCLADKVDAAVLVPSCPVCHQTTALVARHLEANGIATVVMGCAKDIVEHAAV
                     PRFLFSDFPLGNSAGKPHDEESQALTLELALRLLEAAPGAQTTMQSPLRWSADASWKL
                     DYNNIDRMSPEELARRRAEFDRQKEIARGNRAA"
     gene            complement(1894..>2332)
                     /locus_tag="VM910_15185"
     CDS             complement(1894..>2332)
                     /locus_tag="VM910_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HVI63907.1"
                     /translation="AEADPIAPGDYIYLLAPPEKAGALDRFFVDMQPSTAPDPHLLGD
                     FMVSGEHTLGELAEVYGVKVEADASAMTLADYFDVHLDKAPKEGATLALDTIVLVARS
                     ISGGRVNIVGLRLPEEEDDTLPPTRMETFKQKLSDLWTSVAGI"
BASE COUNT          381 a          759 c          755 g          437 t
ORIGIN      
        1 tcccgacagg aacgccagcg tcgcctccag catggagacg tcgaccaatt gtcccttgcc
       61 ggtctggttg cgctggaaca gcgcgctcga gacgccgaag gctgcggtcg cccccgacaa
      121 cacgtcgcac accgcaaatc ccgcacgcgt cggaccggtt tcttcgtgac cggtgatcgc
      181 catgatgccg ctcatcgcct ggatcttgcc gtcatagccc gggctcaggc gctccgggcc
      241 ggtctggccg aagccggaaa ccgcgcaata gatcagccgc ggattgatct ccgagagtgc
      301 ggcatagccg atgccgagct tgtccatcac gccggggcga aaattctcca tcaccacgtc
      361 ggcttgcgca gcgagctttt tgacgatcgc gatcgcttcg ggcttcgcca gatcgagcgt
      421 caggctgcgc ttgttgccgt tgatcgcctg ccaccccggg gcaagcccgc gctcggccca
      481 ctcccggctg agcggcgtgc ggcgcatgtc ctcgccttcg cgccgctcta ccttgatgac
      541 gtcggcgccg agcagcgcga gctggtagct cgcatagggc ccggccagca cctgcgtgaa
      601 gtccagaatc ttcacgccct cgaacggtcg cgtcacttca tatcctcccc aagagcggca
      661 tcggagggtt tcacctctcc cactgagaga ggtcggattg catcgccagt tgcagtccga
      721 ctgaggggtt acgggctctc gtaagaattg cgcccctcac ccgcgccttc ggcgcgacct
      781 ctcccggctg gggagaggcg aaggccatca cgccgcgcgg ttgccgcgcg cgatctcctt
      841 ctggcggtcg aactcggcgc gacggcgcgc gagctcttcc ggactcatcc tgtcgatgtt
      901 gttgtagtcg agcttccagg aggcgtctgc gctccagcgc agcggcgact gcatcgtggt
      961 ctgcgcaccc ggcgccgcct ccaacagccg cagcgccagc tccagcgtca gcgcctgcga
     1021 ttcctcatcg tgcggtttgc ccgcggaatt gccgagcggg aagtcagaga aaaggaagcg
     1081 cggcacggcg gcgtgctcga cgatatcctt ggcgcatccc atcacgaccg tggcgatgcc
     1141 attggcctcg aggtggcggg cgacgagcgc cgtggtctgg tggcagaccg ggcaactcgg
     1201 caccagcacg gcggcatcga ccttgtcggc gaggcagcgc gcgagaattt ccggcgcgtc
     1261 gacgtcgatg gtcacgcggt ggctgcggtt ggtcggcgcg ccgaaaaagc gcggcgcgac
     1321 tgcaccgatg cgccttttgg cctgcgcctt cagaagctgc ggcagcggaa accaggtgcc
     1381 gctgtcggtc gcactcgtgt gcttgcgatc gtagccgata tgcgagatgc gcagatcgtg
     1441 ctccttcgag gtgtcgccgt catagacctt gtagaatttc gccccgccat tataggccgc
     1501 gccgggaccc tgatcgccct tcgccaggtc atagggtgcg gccgtcgtga cgatggtcac
     1561 gcgcgattgg tcgagcggct tcttcaatgg ctggaacggc gcatcgacat gatgtgccca
     1621 gcggtacggc gtggtgtagc cgattgcgat gtagtaatcg cgcgtccgct gcatgtaggg
     1681 aacgggcgca tcatagtcgg gcgcaaagcc gaattgggca tcatccgggc cggacatttg
     1741 cggtctcctt gcgtttccat cattgacgtg cagactagag atagtcggcc tgttgctcaa
     1801 caggcaggtg gagcggatcc gaggcgtaga gccgcgcgca tgctgagtgc ggcgtcttga
     1861 accatgatgc tggaatttct ctgcagccgg gcctcaaatt cccgcgaccg aggtccatag
     1921 atccgacagc ttctgcttga acgtttccat ccgcgtcggc ggcaaggtat cgtcttcctc
     1981 ctcgggcagc cgcaggccga cgatgttgac cctgcctccg gagatcgagc gggcgaccag
     2041 cacgatggtg tcgagcgcca gcgtcgcgcc ctccttcggc gccttgtcga gatgaacgtc
     2101 gaaataatcg gcaagcgtca tggccgaggc atctgcttcc actttcactc cgtaaacctc
     2161 ggccagttcg ccgagcgtgt gctcgccgga caccatgaaa tcgccgagca gatgcggatc
     2221 gggcgccgtg ctcggctgca tgtcgacaaa gaagcggtcg agcgccccgg ccttttccgg
     2281 cggcgccagc aggtagatgt agtcgccggg cgcgatcggg tcggcttcgg cc
//
LOCUS       DATLJT010000654         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_269955,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000654 DATLJT010000000
VERSION     DATLJT010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269955"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VM910_15190"
     CDS             complement(<1..591)
                     /locus_tag="VM910_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI63908.1"
                     /translation="MVPELLAEPKHQSPLRWLSNQPYLLLSLTSLFWAGNIVLARYVA
                     GHVPPLTLSCVRWIGVSLILMPFAWPHLKRDWPTLLTHLPMMLLLSATGFAYNNAVSY
                     WAMQYTQALNALLIQSAGPLFVALWSLLLFGVRLTGPQLAGILISLAGVLTILLHGDL
                     SALATIRLNKGDVMFASSLLAFGLYSALMPRRPITHP"
     gene            complement(715..1650)
                     /locus_tag="VM910_15195"
     CDS             complement(715..1650)
                     /locus_tag="VM910_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI63909.1"
                     /translation="MSASDQTSQAASPFHWIANQPYLLLSITALCWAGNAIVGRLAAG
                     HIPPVTLSFLRWSLAFLLILPLAWKHLKRDWPAIRGKLGVMVLLSVTGIGVFNTLQYW
                     ALEHTQALNTLLLQSSGPLIVAVWSLILLGVRLTLAQAIGVLLSLSGVLVILTQGELA
                     VLSAIRFNKGDLIFLVAMITFGLYSVLTLKRPDIHSLSLVGFTFGCGAVVLIPLLIWE
                     LTTRPAMVFDAQNVLSLVYVAIFPSVLAYLCFNRGVQLIGANRAAPFFHVVPVFGAAM
                     AMVFLGEQPQLFHVIGFALVLAGVYIASRKQATKR"
     gene            2124..>3161
                     /locus_tag="VM910_15200"
     CDS             2124..>3161
                     /locus_tag="VM910_15200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVI63910.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLV
                     INGETYKLALLPSGVLRPSKLAVIGNGVVFDPQAFLDEIAKLKSQGVAVSPDNLRIAE
                     NVTLILPLHRELDALRESSNAVTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPH
                     KIDRLLAHHNALRRGLNLEEVDGKGILRELTELAPKLLPYAETVWRLLDLRRREGKRI
                     LFEGAQGALLDVDHGTYPYVTSSNTVAAQAATGTGIGPGGVGYVLGICKAYTTRVGQG
                     PFPTELNNEIGEEIGRRGREFGVNTGRKRRCGWFDAMLVRQTVRTCGINGLALTKLDI
                     LDGFDTIEVCIG"
BASE COUNT          660 a          928 c         1061 g          512 t
ORIGIN      
        1 cgggtgagtg atcggccgcc gcggcatcag cgccgaatag aggccgaatg cgagcagcga
       61 gcttgcaaac atcacgtcgc ccttgttgag gcggatggtt gcgagcgccg agagatcgcc
      121 gtgcaacagg atcgtgagga cgcccgccag cgagatcagg atgccggcga gctgagggcc
      181 ggtcaggcgc acgccgaaca acaacagcga ccacaacgcg acgaacagag gaccagcgga
      241 ttggatcaac agagcgttga gcgcctgggt atattgcatg gcccagtagg agaccgcgtt
      301 gttgtaggca aatccggttg ccgataacag cagcatcatc ggcagatgcg tgagaagcgt
      361 cggccaatcg cgcttcagat gcggccaggc gaacggcatc aggatcaggc tgacgccgat
      421 ccaacgcacg caggacagcg tcagtggtgg cacgtgacct gcgacatagc gcgcgagcac
      481 gatgttgccg gcccagaaca gcgaggtcag gctgagcagc agataaggct ggttggagag
      541 ccagcgcagc ggggattggt gtttcggttc ggcgagcaat tcaggaacca tatgtcccgg
      601 aaacttttgt cgaaacaaga ttgcggcgcg aattgctgcc atgcggggca actcatccgt
      661 cgccgagagc gcggacgcgt acctcgcccg agctgccata tccgtggtcg aagatcagcg
      721 cttggtcgcc tgcttgcgcg aggcgatata aacgccggcc agcaccagtg caaagccgat
      781 gacatgaaag agttgcggct gctcgccgag aaacaccatt gccatcgccg caccgaatac
      841 cggcacgaca tggaagaagg gcgcggcgcg gttggcaccg atcaactgca cgccgcgatt
      901 gaagcagaga taggcgagca ccgagggaaa gatcgcgacg tagacgagac tcagcacgtt
      961 ctgcgcatcg aacaccatcg ccggtcgcgt ggtgagctcc cagatcaaga gcggaatgag
     1021 gacgaccgcg ccgcagccaa aggtgaagcc gaccagggag agagagtgaa tgtccggacg
     1081 cttcagcgtc agcaccgaat aaagcccgaa ggtgatcatc gcgacgagga agatcaggtc
     1141 acccttgttg aagcgaatcg ctgacaggac ggcgagttcg ccttgcgtca ggatcaccag
     1201 cacgccgctc agcgacagca gaacgccgat cgcctgtgcg agcgtcagcc gtacgccgag
     1261 caggatcagg gaccaaaccg cgacgatgag cggccccgac gattgcagga gcaaggtatt
     1321 cagtgcctgc gtgtgttcga gcgcccagta ttgcagcgtg ttgaagacgc caatgccggt
     1381 gaccgacagc agcaccatga cgccgagttt gccgcggatc gccggccagt cgcgcttcaa
     1441 atgcttccat gccaagggca gaatgaggag gaaggcgagc gaccagcgca ggaacgacag
     1501 cgtcacggga ggaatgtggc cggcggcaag gcggccgacg atggcgttgc cggcccagca
     1561 cagcgccgtg atcgagagca ggagataagg ctgattggcg atccagtgga acggcgatgc
     1621 agcctgcgaa gtttggtcgg aagcggacat cgggggttat cgttatgtgg cacggttcaa
     1681 tgcgccgtgc tccgcgcccg gcgcctggtt ccgggccggt tttttgcccg gctgccccac
     1741 agacacggat tccgaggccg catcaatggg gtccgatgca tcgcgaccat gcgcgcccgc
     1801 ccttgtcgcg agcggcgaat cgaatgccgg catgaggcgc aggagattgc tttaggttaa
     1861 tgttcgctgc cacggtgccg acacaaattt ttaggggata tcaagggctt ggaccggcac
     1921 ctgaaagcca aaaaacgccc ttcttgcttg cctagatcaa ctgcttcctt tatccggtgg
     1981 gtagcctcgc atgcgagcgg gcccggccgc tatttcggcc gggtgtatga tcgggacaag
     2041 gcctgtgtcg gacgtgtttt ggggcgcgcc ggctttccga accgatagct cgagtgacga
     2101 ggtttcaggg gactagatcg cgaatggcca acgttgtcgt cgtcggcgcc caatggggcg
     2161 acgaagggaa gggcaagatc gtcgattggt tgtcggagca ggccgatatc gttgtgcgct
     2221 ttcagggcgg ccacaatgcc ggccacacgc tcgtcatcaa cggcgaaacc tacaagcttg
     2281 ccctgctgcc gtccggcgtg ctgcggccct cgaaactggc cgtgatcggc aacggcgtgg
     2341 tgttcgatcc gcaggcgttc ctcgacgaga tcgcgaagct gaagtcccag ggcgtagccg
     2401 taagcccgga caatctgcgc atcgcggaga acgtgaccct gatcctgccg ctgcatcgcg
     2461 agctcgatgc cttgcgcgaa tcctccaatg ccgtcacggc gatcggcacc acgcggcgcg
     2521 gcatcggccc ggcctatgag gacaaggtcg gccgccgcgc catccgcctg atggaccttg
     2581 cagacctcga tacgctgccg cacaagatcg acaggctgtt ggctcaccac aacgcgttgc
     2641 ggcgcgggct caatctggag gaggtcgacg gcaagggcat tttgcgcgaa ctgactgaac
     2701 tggcgccaaa gcttctgcct tatgccgaaa cggtgtggcg tctgctcgac ttaaggcggc
     2761 gcgagggcaa gcgcatcctg ttcgagggcg cccagggcgc gcttctcgat gtcgaccacg
     2821 gcacttaccc ctacgtcacg tcctccaaca cggtggcggc gcaggcggcg accggaaccg
     2881 gcatcggacc gggtggggtc ggctatgtgc tcggcatttg caaggcctac acgacccggg
     2941 tcggccaggg gccgttcccg accgagctta acaacgagat cggcgaagag atcggccgca
     3001 ggggccggga gttcggcgtc aacaccgggc gcaagcggcg ctgcggctgg ttcgacgcga
     3061 tgctggtgcg ccagacggtc aggacctgcg gcatcaacgg gctggcgctg accaagctcg
     3121 atattctcga cggcttcgac acgatcgagg tctgcatcgg c
//
LOCUS       DATLJT010000655         2457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1877793,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000655 DATLJT010000000
VERSION     DATLJT010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877793"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VM910_15205"
     CDS             complement(<1..458)
                     /locus_tag="VM910_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVI63911.1"
                     /translation="MRRQILPSLLVGAAMLVSSELRAQQPAAPNLDAVPEKMPFSTAY
                     GPPIGAKRADAVIEAAVAEANKRGWAMNIAVVDSGANLVAFLRMDDAQLASIPIAEHK
                     ARAAVKYRRPTKAFEDVVQKFDFKYILTLDDVIASRGGIPLIENGKIIGAV"
     gene            706..>2457
                     /locus_tag="VM910_15210"
     CDS             706..>2457
                     /locus_tag="VM910_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVI63912.1"
                     /translation="MRPLLFLATKILSTTFAFAAFAQAPAAPPHNVVLFVADGLRFRM
                     VDDRTAPTMAAIAREGVSLRNSHALFPTFTTANASGMATGHMLGDTGDFSNTIYTGFE
                     VPSAANSSTPFLENDAVLGEVHAHFSGNYLDEATILKLARDKGYSTASIGKLGPALIF
                     DSTERSGGQTILIDDATGTPKGIPLSAEVTQRLQAVNLPPAAPSRGANGAAGNVSTPG
                     TTNANVVQQDYFAAVTTRVVLPMFKDRNKPFLLVFWSRDPDGTQHNQGDSLNALVPGI
                     NGPTSLAAIRNTDDDLARIRSALSELGLLDSTNIIVTSDHGFSTISKESQTSSTVATK
                     FSDTLPGHLPFGFVALDLAHALNLPLIDPDDGYKIIGEGQHTKNGNGLIGGDRNKPQI
                     VVAANGGSDLVYIPDGDTATAKRVVDVLLTQDYVSGIFVDSKLGKFPGTLSLDDIALE
                     GAAITPRPAIAISFRSFDTVCGEPVLCTVEVADTALQQGQGMHGSFSRADTWNFMAMQ
                     GPDFKSQFVDPAPASNADLGRTIAHLMQLEVKDNGKLVGRVLSETLPNGAVPAVTSRV
                     LVSDPAPNGLATVVNMQM"
BASE COUNT          475 a          820 c          715 g          447 t
ORIGIN      
        1 acggcgccga tgatcttgcc gttctcgatc aacgggatgc cgccccgcga ggcgatgaca
       61 tcatcgagcg tcaagatgta cttgaaatcg aacttttgca ccacgtcttc gaaggccttg
      121 gtcggacggc ggtacttgac cgcggcgcgc gccttgtgct cggcgatcgg gatcgaagcg
      181 agttgcgcat cgtccatgcg caggaaggcg acaagattgg cgcccgagtc gacgacggcg
      241 atgttcatcg cccaacccct cttgttggct tccgcaacgg cggcctcgat caccgcgtcg
      301 gcgcgtttcg ccccgatcgg cggtccatag gcggtgctga acggcatctt ttcaggaacg
      361 gcgtcgagat tgggcgcagc cggttgctgg gcgcgcaact cggaactgac cagcattgcc
      421 gcacccacaa gcagtgacgg caaaatctgc cttcgcatgg ataacctccc caatactgtc
      481 gtttgaagac tactatcgca cgagagttca gcgcgccgtt gaaaacagcg cgcacgagcg
      541 actttataga accgggcctt acgcctcaac ccgccagtcg gcctccgcgc gaccgggaac
      601 gtccaccgtt gggatggacg caaaaccgac ccacgcaacg gccgtcacat gactgtcatg
      661 ggcgaccgtc agtcttgatt ctctccctaa accttggagc tgcgcatgcg cccgcttctc
      721 ttcctcgcca cgaaaatcct gtcgacaacc tttgcgttcg cggctttcgc gcaagcaccg
      781 gcggcgccac cgcacaatgt cgttttgttc gtggccgacg gattgcgttt ccgcatggtg
      841 gacgaccgca ccgcgccgac catggcggcc attgcgcgcg agggggtgag cctgcgcaac
      901 agccacgcgc tgtttccgac cttcaccacc gcgaacgcct cagggatggc gaccggccac
      961 atgctgggcg acaccggtga tttctccaat acgatttaca cgggtttcga agttcccagc
     1021 gccgccaata gctcgacgcc gtttctcgaa aatgatgcgg tcctcggtga ggtgcacgcg
     1081 cacttttccg gcaattacct cgacgaggcg acgatcctga aactcgctcg cgacaagggc
     1141 tacagcacgg cttcgatcgg caagctcggc ccggcgttga tcttcgactc gacggaacgc
     1201 agcggcgggc aaacgatact gatcgacgat gcgaccggca cgcccaaagg catcccgttg
     1261 tcggccgaag tgacccaacg cctgcaggcg gtgaaccttc cgcccgcggc gccttcgcgc
     1321 ggggcaaacg gcgcggccgg caatgtgagc acgccgggga cgaccaacgc caatgtcgtg
     1381 cagcaggatt atttcgcggc ggtgaccaca cgcgtcgttc tgcccatgtt caaggaccgc
     1441 aacaagccgt ttcttctggt gttctggtcg cgcgaccccg atgggaccca gcacaatcag
     1501 ggcgacagct tgaacgccct cgttcccggc atcaacggac cgacttcgct cgccgcgatc
     1561 cgcaatacgg acgacgatct cgcccgcatc cgctccgcac tgagcgaact cggcttgctc
     1621 gactccacca atatcatcgt cacctcggat cacggcttct cgacgatctc gaaggaaagc
     1681 cagacaagct cgaccgtagc aaccaagttt tccgacacgc ttcccggcca tctgcccttc
     1741 ggcttcgtcg cgctcgatct cgcgcacgcg ctcaacctgc cgctgatcga tcccgacgac
     1801 ggttacaaga tcatcggcga gggccagcac accaaaaatg gtaacggcct gatcggtggc
     1861 gaccgcaaca aaccgcagat cgtggtggcg gcgaatggcg gttccgacct cgtctatatc
     1921 cccgatggcg acacggcgac cgccaagcga gttgtcgatg tcttgctgac acaggactat
     1981 gtcagcggca tcttcgtcga ttcaaaactc gggaaattcc ccggcacgct ctcgcttgac
     2041 gacatcgcgc tcgagggagc ggccatcacg ccccgtccgg cgatcgcaat cagtttccgc
     2101 tcctttgaca cggtatgcgg cgagccggtt ctttgcacgg tcgaggttgc cgatacggcg
     2161 ctgcagcagg ggcaaggcat gcacggctcg ttcagccggg ccgacacctg gaacttcatg
     2221 gcgatgcagg ggccagactt caaatcgcaa ttcgtcgatc ccgcgccggc cagcaacgcc
     2281 gatctgggcc gcacgattgc gcatctgatg cagctcgagg tcaaagacaa cggcaagctg
     2341 gtcggtcgcg ttctctccga gacgctgccg aacggtgccg tgcccgcggt caccagccgc
     2401 gttctcgtgt cggacccagc gcccaacggg ctcgccaccg tggtgaacat gcagatg
//
LOCUS       DATLJT010000656         3055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2107498,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000656 DATLJT010000000
VERSION     DATLJT010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107498"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1616)
                     /gene="secD"
                     /locus_tag="VM910_15215"
     CDS             complement(15..1616)
                     /gene="secD"
                     /locus_tag="VM910_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVI63913.1"
                     /translation="MLYFTRWKALGIILTALVVCLCAVPNFFPEATVKRWPLWAQRRI
                     VLGLDLQGGSYLLLELDSNYVKKEKLDQVRDDVRRTLRDAKIGYTGLAVRNDAVEVRI
                     SKDTDQQTALAKLRELSQPLGGLLGSNGQRSLEVTDGGGGLIRLTVPQAAITDRIRQT
                     IEQSIQIVERRVNQLGTVEPLIQRQGTDRILVQVPGLQDPTQLKTILGKTAKMEFRMV
                     DTSVPADQAQRGNQPPDVEVLMSASAPKVPYVVKKQVLVSGGDLTDAQPGFDQRTGEP
                     IVSFRFNTTGSRKFAQATSENVGQPFAIILDNEVISAPVIREPITGGSGQISGNFTVQ
                     SANDLAILLRAGALPAPLTPIEERTVGPGLGQDSIEKGELAAYVGSIMVIVFMLVTYR
                     LFGVFANVAVIINVAMIFGLLSLLNATLTLPGIAGIVLTVGIAVDSNVLIYERIREEL
                     RGGRTAISAIDAGFRRALSTILDSNITTFIAAAVLFYIGTGPVRGFAVTLGIGIITTV
                     FTAFTLTRLIVAWWVRWKRPQSVPI"
     gene            complement(1676..2041)
                     /gene="yajC"
                     /locus_tag="VM910_15220"
     CDS             complement(1676..2041)
                     /gene="yajC"
                     /locus_tag="VM910_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVI63914.1"
                     /translation="MLITPAFAQAAAGGDTNSMLMSLLPFALIFVIMYFLILRPQQKK
                     VRDHADLVKNIRRGDTIVTSGGLVGKVTKVVDDDQIEFEISDGVRVRQMRQMISGVRS
                     KGEPAKEKTDIVKDETSAS"
     gene            2223..>3055
                     /locus_tag="VM910_15225"
     CDS             2223..>3055
                     /locus_tag="VM910_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI63915.1"
                     /translation="MSRKPRKTASRPASRAAAERTATIAAKRPTTVRNDDTPARIARA
                     LEAIAAHLSASASAPPGPETFANADAFVWHPNGRLTDVPRVSRVEIGLLKGIDRMRDI
                     LIENTERFAGGLPANNALLWGARGMGKSSLVKATHASINVDREPKDRLKLIEIHREDI
                     ESLPSLMELLRASDFRFIVFCDDLSFDGNDASYKSLKAVLEGGIEGRPDNVILYATSN
                     RRHLLAREMIENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQDEYLAMVKGYCSH
                     FG"
BASE COUNT          599 a          953 c          955 g          548 t
ORIGIN      
        1 caggccgaag ctccctagat cggcacgctt tgcggccgtt tccaccgcac ccaccacgcc
       61 acgatgaggc gggtaagcgt gaatgcggtg aaaaccgtgg tgatgatgcc gatgccgagc
      121 gtcacggcga agccgcgcac cggtccggtg ccgatataga acaggaccgc agcggcgatg
      181 aaggtcgtga tgttggaatc gaggatcgtc gagagtgcgc gcctgaagcc ggcgtctatc
      241 gcggagatgg cggtccgccc gccacgcaac tcctcccgga tgcgctcata gatcaggacg
      301 ttggaatcga ctgcgatgcc gacggtcaac acaatgccgg caatacccgg caaggtcagc
      361 gtcgcgttca acagggacag gaggccgaag atcatcgcga cgttgatgat cacggcgaca
      421 ttggcgaaca cgccgaagag ccgataggtc accagcatga agacgatgac catgatcgag
      481 cccacatagg ccgcaagctc gcccttctcg atcgaatcct gtcccaggcc cggaccgacg
      541 gtgcgctcct cgatcggcgt caaaggagcc ggcagcgcgc cggcccgcag caagatcgcg
      601 agatcattgg ccgactgcac ggtgaaattg ccggaaatct gccccgaccc gccagtgatc
      661 ggttcgcgga tcaccggggc ggaaatcacc tcgttgtcga gtatgatggc aaagggctgt
      721 ccgacatttt ctgaagtggc ttgcgcgaat ttgcgggagc ccgtcgtgtt gaagcggaag
      781 ctcacgatcg gctcgccggt tcgctggtca aagcccggtt gcgcatcggt caggtcgccg
      841 cccgagacca gcacttgttt cttgacgaca taaggcacct ttggcgccga ggcgctcatg
      901 aggacctcga cgtcgggcgg ctggttgccc cgctgcgcct ggtccgccgg cacgctggtg
      961 tcgaccatgc gaaattccat tttcgctgtc ttgccgagga tggtcttgag ctgggtcggg
     1021 tcctgcagac ccgggacctg cacgagaatg cggtcggtgc cctggcgctg aattaagggt
     1081 tccaccgtgc cgagctggtt gacgcggcgt tcgacgattt gaatcgactg ctcgatggtc
     1141 tggcggatgc gatcggtgat tgcagcctgc ggaacggtga gccggatcaa cccgccgccg
     1201 ccgtcggtga cctcgagcga gcgctgaccg ttagagccga gcaagccacc gagcggctgc
     1261 gacagctcgc gcaatttcgc gagcgcggtc tgctggtcgg tgtctttgct gatacgcacc
     1321 tcgaccgcgt cattgcggac cgcaaggcca gtgtagccga tcttggcatc ccgcaaggtc
     1381 cggcgcacat cgtcgcgcac ctgatcaagc ttttccttct tcacgtaatt ggaatcgagt
     1441 tccagcagca ggtacgagcc gccctgcaaa tcgaggccga gcacgattcg gcgctgcgcc
     1501 cagagcggcc agcgcttcac ggtggcctcg gggaagaaat tcggcacggc gcagagacac
     1561 accacaagcg ccgtcagaat gatgccgagc gccttccacc gcgtgaaata caacatcgag
     1621 tcgactcgtc aggtcttgaa ggaaattacc gcgcgccaga tgcgccgatc gccgatcagc
     1681 tcgcggaggt ctcgtccttg acgatatccg tcttctcctt ggcaggttct cccttggagc
     1741 ggacgcccga gatcatctgc cgcatctgcc gcacgcgaac gccgtcggag atttcgaact
     1801 cgatctgatc gtcatcgacg actttggtca ccttgcccac gaggccgccc gaggtgacga
     1861 tggtgtcgcc gcggcgaatg tttttgacga gatcggcgtg atcccgcact ttcttctgct
     1921 gcggtcgcag aatcaggaaa tacatgatca cgaagatcag cgcgaatggc agaagcgaca
     1981 tcaacatgct gttggtatcg ccgccggccg cagcctgggc aaacgcaggg gtgatcagca
     2041 tcaggacaat cctcgtgaaa cggaaggccg gcaaggccgc cttcgcggcg ggccggttcg
     2101 ggcgaattcg cgcggactat agcggccgcg gtcccaattg caacgccgcg cagacccccg
     2161 atttggcccg cttgcgggag gcatcaaggc ccgataaggc tgcacagtca ggaacttgag
     2221 ccatgtcgag aaaaccccgc aaaaccgcgt cccggcccgc ttccagagct gccgccgaaa
     2281 ggaccgcaac tatcgcggca aaacgcccca cgaccgtccg taacgacgat acgccggcac
     2341 ggatcgcgcg tgcgctggag gctattgcgg cccacctgtc ggcgagcgcg tcggcgcctc
     2401 ctgggcctga gacgttcgcc aacgccgatg cctttgtctg gcacccgaac gggcgattga
     2461 cggatgttcc gcgcgtcagc cgcgtcgaga tcggcctgct caaaggcatc gatcgcatgc
     2521 gcgacatcct gatagagaat accgaacgct tcgctggcgg cttgcccgcc aacaacgcgc
     2581 tgttgtgggg cgcgcgcggc atgggcaagt cttcgctggt caaggctacc catgccagca
     2641 tcaatgtcga ccgcgagcca aaggaccgtc tgaagctgat cgagatccac cgcgaggata
     2701 tcgagagcct cccctccctg atggagcttt tgcgggcgtc cgacttccgc ttcatcgtgt
     2761 tctgcgacga cctgtccttc gacggcaacg acgcctccta caagtcgctg aaggccgtgc
     2821 tggaaggcgg catcgagggc cgtcccgata acgtcatcct ttacgcgacc tctaacaggc
     2881 gtcatctgct ggcgcgtgag atgatcgaga acgagcgctc aaccgcgatc aatcccggcg
     2941 aggcagtgga ggaaaaggtc tcgctgtccg atcgattcgg cttgtggctc ggctttcacc
     3001 gctgcagcca ggacgaatat ctggcgatgg tgaaaggcta ttgcagccac ttcgg
//
LOCUS       DATLJT010000657         3492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1648554,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000657 DATLJT010000000
VERSION     DATLJT010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648554"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VM910_15230"
     CDS             <1..537
                     /locus_tag="VM910_15230"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63916.1"
                     /translation="TDVIAPAAGLKKGDFQEVRMDVNNMVAAMAAKTVDAMVNVEPYN
                     EIAVAEGIGTSIMDYSKVDKMPVFMAATPDFVDKNPDTVVAYLKCWQEAARDFKDNPG
                     KVADVIYAFFTSKGYAMSRETFAKALAKVEVDPGFPSDLVPGLQKDAEVLLREKKISA
                     IPDWNKALRPELWAKARG"
     gene            1205..1699
                     /locus_tag="VM910_15235"
     CDS             1205..1699
                     /locus_tag="VM910_15235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVI63917.1"
                     /translation="MLTTAIVAELPRLRRHALYLSYNHADADDLVQDCLEAALSKKAS
                     LQDPAKLRSWLFSILNNFFLMRLRSEARRGQTLQIEDFADSLAASASSDDRQLARDLA
                     RAMGKLSAEHRQILLLLNVEGYGYQEVADTLGIPIGTVMSRLARARRRLRALLEGHEL
                     QVVD"
     gene            1704..2444
                     /locus_tag="VM910_15240"
     CDS             1704..2444
                     /locus_tag="VM910_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63918.1"
                     /translation="MSIAPITEQELHAYLDGELPDARRAAVEAYLREHPEDARRLEAY
                     RADGKAIARIFSRAGAATPGTRPSSSSSWRSVPWRLAAAAVLILAVGSVAGWFGRERV
                     NDTSMERLGHQAAAAHLILNSPGVEPLATSSLDELSRSMSSTLGTRVRLRDPSSTGYK
                     IVGARIVPQGEGRAVQLVVRGPADETITFYIEGRPGVKETPFRRIAGEGLTTLVWEDD
                     DLACAITSTLEPKKLEEVGRRIYDALLG"
     gene            complement(2483..>3492)
                     /locus_tag="VM910_15245"
     CDS             complement(2483..>3492)
                     /locus_tag="VM910_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVI63919.1"
                     /translation="VVWNNDHSGVLNRFGQPCPERYGLRTVENVLMALREFGHDALLC
                     EGDKGMLAALERFMPPDRQGRPTGMVFNMAYGIQGECRYTHVPAMLEMAGVPYTGSSP
                     LGHALALDKVITKQLIRDAGVSTPNFCVMRRGSEYAGGLRFPLVVKPRHESTSFGLQL
                     VREPSQLSDAVEAIVAQYEQDALVEEYIEGREICVALLGNEELEILPLVEQAFGDREM
                     HLITWEDKTHRAAAEPQKICPAQVSNTLASILRDISIATFRACRCRDYARVDIRIDDS
                     GQPYVLEINSMAALGMAASYVLAAKTAGLSFSALVNRILDVAHRRYFGMDVGEYALEC
                     A"
BASE COUNT          698 a         1040 c         1109 g          645 t
ORIGIN      
        1 accgacgtca tcgcgcccgc cgccggcctc aagaagggcg acttccagga ggtgcgcatg
       61 gacgtcaaca acatggtcgc ggccatggcc gccaagaccg tggacgccat ggtcaacgtg
      121 gagccctaca acgagatcgc ggtcgccgag ggcatcggca cctccatcat ggattactcc
      181 aaggtcgaca agatgccggt gttcatggcg gcgacgcccg acttcgtcga caagaacccg
      241 gataccgtcg tcgcctatct caagtgctgg caggaggcgg cgcgcgactt caaggacaac
      301 cccggcaagg tggccgacgt gatctacgcg ttcttcacgt cgaagggcta cgccatgtcg
      361 cgcgagacgt ttgccaaagc actggccaag gtcgaggtcg atccgggctt cccgtccgac
      421 ctcgtgccgg gcctgcagaa ggacgccgag gtgctgctgc gcgagaagaa gatttccgcc
      481 attcccgatt ggaacaaggc gctgcggccg gagctatggg cgaaggcgag gggctagggc
      541 gtgaactcat aaacgcgggc attgggcgaa tgtccgctct tggggcgaac cggacacgcc
      601 gggatggcgg gaatgacgtg aatgacccaa agcggacaag gtcggatcga gatcctgcgg
      661 tgcggcagcg gccgcatccg acattcgtgg tttgggtcct cctgggggag gcacagcatg
      721 cgtgcccaat aaattctagc gccaatacgg gaataggacg ggatttcgat gagtctcctg
      781 gttacttcgc catcctcgac ggctcgccag ggcctttaat ccccgttgag aagaattttt
      841 cacgatgggc gggagtctgg aatgaagaat ttttcacaat gggctggagt ttggaataaa
      901 gcgagactgg gaagcatcct taccatggta tgggcagcct tacctgcccc ggcggagcgg
      961 tggacagagg gggtagcggc acgaacacgc aggtttgggg cggataaatc gatgtgctgg
     1021 cccaacaacg aactttcaaa gcaagcccga gttatggggc tcggggcggg tacaccattg
     1081 cggggcatat ggaactttga tcgggatcaa aatgtcctga gcaccattga gctagctaaa
     1141 gctcatggtc tcggatcaac ccagagcggt gggacgggcg ccgcggtccg acgcaacaga
     1201 cgaaatgttg accaccgcca tcgtggccga actgccgcga ttgcggcgac atgccctcta
     1261 tctttcttac aaccacgcgg atgccgacga tctggtccag gactgcctcg aagcagccct
     1321 gagcaagaag gcgtcgctgc aagatcccgc gaagctgcgc agttggctgt tctcgattct
     1381 caacaatttc tttctcatgc ggctgcgttc cgaagcgcgg cgtgggcaaa cgttgcagat
     1441 cgaggatttc gcggacagcc tcgcggcatc ggcctcgtcg gacgatcggc aattggctcg
     1501 cgatctcgcc cgcgcgatgg gcaagctctc cgccgaacat cgtcagattt tgctccttct
     1561 gaacgtcgaa ggatatggct accaggaggt cgcggacacc ttgggaattc caattggaac
     1621 agtcatgtcg cgcttggcga gggcccgtcg ccggcttcgg gcattgctgg aaggccacga
     1681 attacaggtg gtcgactgat atgatgtcga tcgcgcctat cacggagcaa gaacttcacg
     1741 cctatctgga cggcgagttg ccggacgcgc ggcgcgcggc tgtcgaagcg tacctgcgcg
     1801 agcatccgga ggacgcgcgc cggctcgaag cctatcgcgc cgatggcaag gcgattgccc
     1861 ggattttctc ccgcgcgggc gccgcaacgc ccgggacgcg gccttccagc tcaagttcat
     1921 ggcggtccgt cccgtggcgg ctggcagctg ctgcggtact gatccttgcg gtggggtccg
     1981 ttgcgggctg gttcggccgc gaacgcgtca acgatacaag catggaacgc ttgggacatc
     2041 aggcggccgc cgctcatctc attctgaata gtccaggcgt agagccactc gcgacatcgt
     2101 cgctggatga gctctcgcgc agcatgtcga gcacgctcgg aactcgcgtg cgactgcggg
     2161 atccaagcag cactggctac aaaatcgtcg gcgcccgcat cgtgccgcag ggagaggggc
     2221 gcgcggtgca gcttgttgtg cgcggccccg ccgacgagac gatcacgttc tacatcgagg
     2281 ggaggcccgg cgtcaaggaa accccctttc gccgcatcgc gggcgagggc ttaaccacgc
     2341 tagtgtggga agacgacgat ctcgcctgcg cgattaccag cacgctggag ccgaagaaac
     2401 tagaagaagt cggccggcgc atctacgacg cgcttctggg ttgagcgcag caaagctgaa
     2461 ggctcgccgg cggccgttag ggctaggcgc actccagcgc atactcaccg acatccattc
     2521 cgaaataccg cctgtgggca acgtcgagga tgcgattcac tagggccgaa aagctcaatc
     2581 cggcggtctt ggcagccagc acgtaggacg ctgccatgcc gagcgcggcc atggagttga
     2641 tttccagcac gtagggttgc ccggagtcgt cgatccggat gtcgacgcgc gcgtaatctc
     2701 tgcaccggca ggcgcggaag gtcgcgatcg aaatatcccg taatatcgaa gcaagagtgt
     2761 tgctaacctg tgccggacaa atcttttgcg gctcggcggc ggctctgtgg gttttgtctt
     2821 cccaggtaat gaggtgcatt tcgcggtcgc cgaaagcttg ctctaccaac ggcagaatct
     2881 cgagctcctc gtttccgagc agcgcaacgc aaatttcccg gccttcgatg tattcttcga
     2941 ctagcgcgtc ctgctcgtat tgggcaacga tggcctccac tgcgtcgctc aattgcgagg
     3001 gttcccgcac caattgcagg ccaaagctgg tggactcgtg gcgcggcttc accacgagag
     3061 ggaaccgcaa gcctccggca tattcgctgc cacggcgcat cacgcagaag tttggcgtcg
     3121 acaccccggc atcgcggata agctgcttgg tgatcacttt gtcgagcgcc agcgcgtgac
     3181 cgagcgggct cgacccagtg taggggacgc cggccatctc cagcatggcc gggacatgcg
     3241 tgtagcgaca ctcgccctga attccatagg ccatattaaa gaccataccc gtgggacggc
     3301 cttgccgatc gggcggcatg aaccgctcga gcgcggcgag cattcccttg tcgccctcgc
     3361 agagcagcgc atcgtggccg aattcccgca gcgccatcag tacgttttca acagtcctga
     3421 gcccgtaccg ctccggacac ggctgaccga atcgattgag cacgccggaa tgatcgttgt
     3481 tccacacaac tg
//
LOCUS       DATLJT010000658         1710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3487026,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000658 DATLJT010000000
VERSION     DATLJT010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487026"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..>1710
                     /locus_tag="VM910_15250"
     CDS             173..>1710
                     /locus_tag="VM910_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63920.1"
                     /translation="MRGLTAALRGLAAALAAACLATPACAHGFGQRYDLPIPLSFYLV
                     GAAAAVVVSFVIVGLFVREAPRVHAYPRIDLIAAPLGRWLASPSLVLVLKFLALAGFI
                     ITILAGFRGDQNPYRNIAPTMVWTIGWVGLAYVSAFVGNVWAVINPWRTIFESIETID
                     RGISGRPEFGLRLPYPATLGVWPAFILLLAFSWVELVYPNPAVPYFIAWLALAYSLLT
                     FAGMFLFGCECWLERGEVFTAVFGTFARFAPIEIRTGARPGMWLRPFGAGLIESGAVS
                     TSMMAFVLLLLSSVLYDGALGTPEWGNLEGVIAAHLSALGDFKLIAIRTAGLFVFWLV
                     FCGAYVGVSAIMSAVTQGRLAPLVFARGFAFTLVPIAIGYHFAHYFTFLLIQGQYIIP
                     LASDPFGFGWNLFGTAGYRVDIAIVDARFAWFTAVTAILIGHIAAVYLAHAKAMQVLD
                     TRGAALRSQIPLTALMVVYTFVSLSILAEPIVERRAPAQPVAVDIDVPEDALLPEPGS
                     GRLQ"
BASE COUNT          230 a          574 c          532 g          374 t
ORIGIN      
        1 ctcaaaccgg gcacggtcac ggagatgacc ttcaccgcgc gcgcgaccgg gcgattcacg
       61 gtcgagcccc acatcgggag aacgccgtcc ggcgggcatg cgcatgggga cgtccttgtc
      121 accatcgagg tctatccgta agcgcagcag acagcgcgca aaggcggttt cattgcgcgg
      181 cttgacggct gcgctgcgcg gcttggcggc tgccctcgcg gccgcatgtc ttgcaacgcc
      241 ggcctgcgcg cacggcttcg gccaacgcta tgatctcccg atcccacttt cgttctacct
      301 cgttggcgcg gccgctgccg tcgtggtgtc attcgtcatc gtcggactgt tcgtgcgcga
      361 agcaccgcgc gtgcacgcct atccgcgcat cgacctcatc gccgccccat tgggtcgatg
      421 gctcgcaagc ccatccctcg tgcttgtgct caagttcctc gcgctcgccg gtttcatcat
      481 cacgatcctc gcggggtttc gcggcgacca gaacccttac cggaacatcg cgccgaccat
      541 ggtgtggacc atcggctggg tcggtctcgc ctatgtttcc gcgttcgttg gcaatgtctg
      601 ggcggtgatc aatccctggc ggacgatctt cgaatcgatc gaaaccatcg accgcgggat
      661 cagcgggcgg cccgagttcg gcctgcgcct gccctatccg gccacactcg gcgtctggcc
      721 cgcgttcatt ctgctgctgg cgttctcgtg ggtcgagctc gtctacccaa accccgccgt
      781 gccgtacttc atcgcctggc ttgcccttgc ctattccctt ctgaccttcg ctgggatgtt
      841 cctgttcggc tgcgagtgct ggctcgagcg cggcgaggtt ttcacggcgg tgttcggcac
      901 attcgcgcgt tttgcgccaa tcgagatacg aaccggcgcc cgtcccggaa tgtggctgcg
      961 cccgttcggc gccgggctga tcgaaagcgg cgcggtctcg acttcgatga tggctttcgt
     1021 gctgttgctg ctctcgagcg tgctctacga cggcgcattg ggcacgccgg aatggggcaa
     1081 tctcgaaggc gttattgccg cgcacctgtc cgccttgggc gacttcaagc tcatagcgat
     1141 caggacggcc gggctattcg tcttctggct ggtgttctgc ggcgcctatg tcggggtgag
     1201 cgccatcatg agcgcggtca cgcaggggcg cttggcgccg ctcgtctttg cacgcggctt
     1261 cgccttcacc ttggttccga tcgcgatcgg ctatcacttc gcgcattatt tcacgttcct
     1321 gctgatccag ggacaataca tcattccgct ggcgtcggac ccgttcggct tcggctggaa
     1381 tctgttcggc accgcgggct accgcgtgga catcgcgatc gtcgacgcgc gttttgcctg
     1441 gttcaccgcg gtgacggcga tcctgatcgg tcacatcgcg gcggtctatc tcgcgcatgc
     1501 caaggccatg caggtcctcg acacgcgagg tgccgcgctg cgctcccaga ttccgctcac
     1561 cgcgctcatg gtggtctaca ccttcgtgag tctttcgatt ctggccgagc cgatcgtcga
     1621 gcgccgcgca ccggcgcagc ccgttgcggt cgacatcgac gtgcccgagg atgcgctgtt
     1681 gcccgagccg ggaagcggtc gattgcagca
//
LOCUS       DATLJT010000659         6930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3716277,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000659 DATLJT010000000
VERSION     DATLJT010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3716277"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..611
                     /locus_tag="VM910_15255"
     CDS             210..611
                     /locus_tag="VM910_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI63921.1"
                     /translation="MFVDCEGDRMHRAMETFFIECTGNGWENWKKADPNVTIQNTHGL
                     VSTNEWTGVPLRFLTDLVGKDRGSTWMLAEGADAAGVDRSIPLTEEIMDEVFIAYGQN
                     GEPLRPAHGFPIRLITPGLEGNLHIKWLCAA"
     gene            593..817
                     /locus_tag="VM910_15260"
     CDS             593..817
                     /locus_tag="VM910_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63922.1"
                     /translation="MALRRLKFGDQPWMTRWETDRYTQLLANGKARQFQLRMETNSVI
                     TQPSGMMEIQPGYNRITGLAWRGIWTSQLR"
     gene            complement(1440..1577)
                     /locus_tag="VM910_15265"
                     /pseudo
     CDS             complement(1440..1577)
                     /locus_tag="VM910_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1800..3194
                     /locus_tag="VM910_15270"
     CDS             1800..3194
                     /locus_tag="VM910_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HVI63923.1"
                     /translation="MSSVTLDWLKGQGQQRANSVIDALSTFEAERLGYDWDFLARPDQ
                     LPPAGNWSVWLYLAGRGSGKTRAGVEWVRRKIKQGSRRIALIAPTTSAARDVLIEGES
                     GILAHAWEHDRDDLGNVIGRPMYEPSKRRLTWGNGAIATAFSAEEPDRLRGPQHDVVL
                     ADELAAWSLIKRESLELQKSYAWDMAMMGLRIGKNPQAMIATTPRPIPILRELLRSPM
                     CVVTRATTYANRRNLAPAFFQHIIAKYKGTRLGRQELEGDILEEAEGALWTRLMVEQA
                     RDGRKSELLRTVVAVDPALSATASSALTGIVVAGRGYDQRVYVLADLSGRFSPDSWAR
                     VAVNAYKQYNADRIVAEGNQGGELVRKTLQTVDANVPIAIVHARHSKQARAEPVAALY
                     EQRRVTHIAPFPELDDQLCTWEPLCGEPSPDRLDAMVWAVSALMFQGEEPKIVQPFVA
                     GTKRYIPGQETSFY"
     gene            3440..4468
                     /locus_tag="VM910_15275"
     CDS             3440..4468
                     /locus_tag="VM910_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI63924.1"
                     /translation="MSLKLKRRAGRPHWYIFGTVRGVSIRESTRVADHKAAEAIRARR
                     EWEIIQRSIFGAEATATFVGAAVSYLEAGGERTYLKPIIARIGSMPLAKIDQAFIEKT
                     ARALYPNAPPATLNRQAFTPIAAVLNHAAKRGLCARKTVERPPQPKGRVRWITFEEAE
                     RLLTACSPHLRPLVMFLLGTGARMSEALYLDWREVDLAGAHVTFLDTKNGEHRGIPLH
                     ARLVNELRGLRHRQGRVFRTNAGFPYAEKESGGGQIKTAFRGACRRAGLADFSPHDCR
                     HTWATWHYAANRDIVALMKLGGWKSERMVLRYAHVNVAQLAPSIDAALAGWAAPNPHQ
                     LIRRQRKH"
     gene            complement(4908..>6930)
                     /locus_tag="VM910_15280"
     CDS             complement(4908..>6930)
                     /locus_tag="VM910_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI63925.1"
                     /translation="VQQRAYIERDPRVVANVQLAGYRSVLAVPMFKENELIGAIVIYR
                     QEVGPFSDKQIALLTNFAAQAVIAIENTRLLNELRQRTGDLSEALERQTATSEVLGVI
                     SSSPGELGPVYRALLENAVRICQAKFAAMFLGNGETFRLTAEHDAPAAWVEQRRSEGV
                     FRPHPDSGLGLIAAKRQVAYIPDLVQQRAYIERDPRVVANVELAGYRSVLAVPMFKEE
                     NELIGAIVIYHQEVRPFSDKQVALLTNFAAQAVIAIENTRLLNELRESLEQQTVTSEI
                     LGVIAASPTNIQPVLEAIAESSCRLCEAYDSVIFLREGERLCGRAHHGPISMRGEGPI
                     DRGWVTGRAFVDREPVHVHDLRAAADEFPDGSERALRFGHRTTLGIPLLREDEAIGTL
                     LIRRSEVRPFTDKQIALLTTFARQAVIAIENVRLFDELQDKNRQVAEASENKSAFVSS
                     MSHELRTPLNVIIGLTEMMVKNAARFGTEKAQEPLQRVNRAGTHLLGLINQVLDLSKI
                     EAGKLELNPQTVQLGPLIKDVIGTAGQLAEQNKNRLVVDTQENLGALTVDPMRLRQIL
                     LNLLSNSCKFTRAGEVKLAARKVSNGSSFVEFAVSDTGIGMTPEQQAKLFEEFTQADA
                     STAQRFGGTGLGLAITRKLARMMGGDVTVTSEPGKGSVFTVRLPGSAES"
BASE COUNT         1344 a         2166 c         2151 g         1269 t
ORIGIN      
        1 acgccaaatt cggcgtccaa aatggcgcgt tttgctggct tttcggcaac accatttcga
       61 ggggtttttt cgtacaccct ggtatggctc gttgcgaaac tcgtccctca accgccctca
      121 acagcgaggg ccgcttctac agggggccga tatttgccgc tgtcaggcgc tgcgcttttg
      181 ggtccggcaa cgccacccag gaggtctgct tgtttgtcga ctgcgaaggg gaccgaatgc
      241 accgcgcgat ggaaacgttc ttcatcgaat gcaccggtaa cggctgggaa aattggaaaa
      301 aggccgaccc gaatgtcacg atccaaaaca ctcatggcct tgtgagcacc aatgagtgga
      361 ccggcgttcc gctgcgcttt ctcacagatc tcgttgggaa ggatcgcgga tcgacctgga
      421 tgttggcgga gggcgccgac gcagccggcg ttgatcggag cattccgctc accgaggaga
      481 tcatggatga ggttttcatc gcgtacggcc agaacggcga accgttgcgg ccggctcatg
      541 gctttcccat ccgtctcatc acgccgggcc tcgaaggcaa tctgcacatc aaatggctct
      601 gcgccgcctg aaattcggcg atcagccctg gatgacgcgc tgggaaacgg atcggtacac
      661 gcagctgctt gcgaacggta aggcgaggca gttccagctt cggatggaaa ccaactcggt
      721 catcacccag ccatcgggca tgatggagat acagcccggc tacaaccgca tcacgggtct
      781 ggcgtggaga ggtatttgga caagtcaact gcgctgacct gattcgcgag acgatagatt
      841 ggtccgtagt ggcaacgtgc ctgcgtgcga gcacaacttg gtcggcaaac gaccgcaagt
      901 ctacgcgagg ctgagctaac agccgcccaa aagaaacggc ctctcgcgta gcttctgacg
      961 gacgcccggt cgacggcgaa ggcgattttt tgtcccacgg cgacacttcg ccagaacttc
     1021 gagttcatcg cgcatccgcg gccgcattac catggctagg tctaaagtct ggtgaggtcc
     1081 gccgtgttct gcggaaacgt ccgcgtcgcc gacatcggaa ttgccgcaag cgtgctcgac
     1141 gagatcaaac cgcgcatctc gatcaacgcg ccgacggatc gagcttcaaa tcggccaagc
     1201 ctcccagccg gcctacgggc gaagtggcgt gagccggccg cggctcgggc aagacctgcg
     1261 ccggcgtcga gtgggtgcga ctcgcgccgc attgcgttga tcgcaccgac cacctcggcg
     1321 gcccgcgacg tgctgatcga ggggcgagag tgggatcctc gcccatgctg ggagcacgac
     1381 cgcgacgacc tcggcaactt catggtcggc cggtgttatg cctcgaccac attcatggcc
     1441 cacggcgcta attcgtcggc aatgaccacg tcgtgttgcg ggccacgtag gcgggcgggc
     1501 tgctcggccg agaacgcggt tgcgatcgcg ccgttgcccc atgtgagccg gcgcttcgaa
     1561 ggggctttcc accttggcgg cagcgaaccg cgcttggttg cttgagcagt ttcgcgagcc
     1621 ggacggcggc ctctacctgg tgggaagcgc gcgcggagtt tccaccagtt cgatgcgcgc
     1681 aaggccggtc ctggcctcta cctcactcct cgtcccatct cgtcacctca ttttgtggag
     1741 gatcacagaa tgacgatcgt ttatcggccg cggccgacac cgacgccagc accccagaaa
     1801 tgtctagcgt cacattagac tggctgaaag gccagggaca gcaacgggcc aacagcgtca
     1861 tcgacgcgct gtcgacattc gaggccgaac gcctcgggta tgactgggat ttccttgcgc
     1921 gtcccgacca gctcccgccc gcgggcaact ggtcggtctg gctctacctc gccggccgcg
     1981 gctcgggcaa gacccgcgcc ggcgtcgagt gggtgcgccg caagatcaaa caaggctcgc
     2041 gccgcattgc gttgatcgca ccgacgacct cggcggcccg cgacgtgctg atcgagggcg
     2101 agagtgggat cctcgcccat gcctgggagc acgaccgcga cgacctcggc aacgtcatcg
     2161 gccggccgat gtatgagccc tcgaagcgcc ggctcacatg gggcaacggc gcgatcgcaa
     2221 ccgcgttctc cgcggaagag ccggatcggc tgcgtggccc gcagcacgat gtcgtgctcg
     2281 ccgacgagct tgccgcctgg tcgttgatca agcgcgaaag ccttgagctt cagaaaagct
     2341 acgcttggga catggcgatg atgggcctgc gcatcgggaa aaacccgcag gccatgatcg
     2401 cgacgacgcc gcggccgatc ccgatcctgc gcgagctttt gcgctcgcca atgtgcgtgg
     2461 tgacccgcgc aaccacctac gccaaccgcc gcaatctcgc accggcgttc ttccagcaca
     2521 tcatcgccaa gtacaagggc acgcgcctcg gccggcagga gcttgaaggc gatatccttg
     2581 aggaagcgga gggtgcgctg tggacgcggc tgatggtgga gcaagcgcgc gatggacgaa
     2641 aatcagaatt gcttcgcacc gtggtggctg tcgatccagc cctcagtgcc accgccagca
     2701 gcgccctcac cggaatcgtg gtggcgggac gcggatacga tcaacgggtt tatgtcttgg
     2761 ctgatctgtc gggccgcttt tctcccgaca gttgggcgcg cgtcgccgtc aacgcctaca
     2821 agcaatacaa cgccgaccga atagtcgcgg aaggcaacca gggcggcgag cttgtgcgca
     2881 agacgctaca aacggtcgac gccaacgtcc cgatcgcgat cgtgcacgcc aggcattcga
     2941 agcaagcgcg agccgagccg gtcgcagccc tttacgagca gcggagggtc acgcacatcg
     3001 cgccgttccc cgagctagac gaccagcttt gcacgtggga accgttgtgc ggcgaaccgt
     3061 cgcccgaccg cctcgacgcg atggtgtggg cagtcagcgc gctcatgttc caaggcgaag
     3121 aaccgaagat cgtgcagcca ttcgtggccg gcacaaagcg ctacattccg ggacaggaaa
     3181 catcgttcta ctgatccggg gcatcgctga catagtcacc ccctttttcc caatgtcgct
     3241 gccgaagaac ctcgtagagg acgaaatagg ccccgatgac taccctagag ttattcaccc
     3301 tggatgaagt cgcggcgacg ttccgcgtga gcccgcgtaa gatgcgcgag cacgttcggc
     3361 tgcatccctt ctaccgtatc ctcggcggcc gcaagctatt cacccgctcc gacatcaaag
     3421 ctctatatga ggcgctcgaa tgtccctcaa gctcaagcga agagccggcc gcccgcactg
     3481 gtacatcttc ggaaccgtcc gaggggtcag catacgcgag agcacacgag ttgctgacca
     3541 caaggccgcc gaagcgatcc gggcgcggcg cgagtgggag atcatccaac gcagtatctt
     3601 tggagcggag gcgacggcga cgttcgtcgg cgcagccgtg agctacctcg aggccggcgg
     3661 cgagcggacc tacctcaagc cgatcattgc gcgcatcggc tcgatgccac tggcgaagat
     3721 cgaccaggcg ttcatcgaga agacggcgcg ggcgctttat cccaatgctc cgccggcaac
     3781 gctcaaccgc caggcgttca cgccgatcgc ggccgtgctc aaccatgccg ccaagcgcgg
     3841 gctatgcgcg cgcaagacgg tcgagcggcc accgcagccg aaggggcgcg ttcgctggat
     3901 cacgttcgag gaggctgagc ggctgttaac tgcatgctcg ccgcacctgc gaccgctggt
     3961 catgtttctg ctcgggacag gagcgcgcat gtccgaggcg ctctacctcg actggcgcga
     4021 agtcgacctc gccggcgcac atgtgacttt cctcgacacc aaaaacggtg agcaccgcgg
     4081 catcccgcta cacgcccggc tcgtgaacga attgaggggc ctgcggcacc gccagggtcg
     4141 cgtctttcga accaacgctg gctttcccta cgcagagaaa gagagtggcg gtgggcagat
     4201 caagactgcg ttccgaggag cgtgccggcg ggcaggactg gctgatttca gcccgcatga
     4261 ttgccgccac acctgggcga cgtggcacta tgccgcaaat cgagacatcg tcgcgctgat
     4321 gaaactcggc ggctggaagt ccgagcgcat ggtgctgcgt tacgcgcacg tcaacgtcgc
     4381 gcagctcgcc ccgtcgatcg acgccgcgct tgccggctgg gcagcaccaa atccgcacca
     4441 actgattcga cgccagcgaa aacattaggc gcgtgaaata gatgcgcgag cacgggcacc
     4501 cccttggtaa gggggaggtc gtaagttcaa tccttaccgg cagcaccaga aatcaaatac
     4561 ttaagccaac tcttccatcg cacttcatga cgtttcgaga cggaacgata catgcattgg
     4621 tgtaaaacca gtgtactctg ttctccgtga gttcgcggag cggtttcgga cgagaggcag
     4681 ccacagacac cagatgtggt ccgcgttgga ccaaaaccgt aagggcgcgc caacagcgct
     4741 gagagatagc gccgaagagc acgactaagg ccgctggaag gcgcttgctc atgtccgctt
     4801 tacccccgaa agcggacatc gagcgacaca tgtctgcttt gtgccataag ccgacatatg
     4861 cagcgcagca aattagcacc ggtctccgca gccatgttgc aacttgttta gctttcggcg
     4921 ctgccaggca ggcgcactgt aaacaccgag cccttgcctg gctcgctcgt caccgtcacg
     4981 tcgccgccca tcatgcgcgc gagcttgcgg gtgatagcaa gaccaagtcc ggtgccgccg
     5041 aagcgctgag cagtcgaggc atcggcttgg gtgaattcct cgaacagctt agcctgctgc
     5101 tcaggcgtca tgccgatgcc ggtgtcggag acggcgaact cgacgaagct actgccgttg
     5161 ctcaccttgc gtgccgcaag cttgacctcg cccgctctgg tgaatttgca cgaattgcta
     5221 aggaggttga gcaggatctg ccgcagccgc atcgggtcca cggtcaatgc gccgaggttc
     5281 tcttgagtgt caacgacgag ccggtttttg ttctgctcgg caagctgtcc ggcggtgccg
     5341 atgacatcct tgatgagcgg acctagctgc acggtttgcg ggttgagctc gagctttccg
     5401 gcctcgatct tggagaggtc gagcacctgg ttgatgaggc cgagcaggtg agtgccggcg
     5461 cggttcacgc gctgcagtgg ctcctgcgcc ttttcggtgc cgaagcgcgc cgcatttttg
     5521 accatcatct cggtcagccc gatgatgaca ttaagcggcg tgcgcagctc gtggctcatg
     5581 ctggagacga aggccgactt gttctcgctg gcctctgcga cctgccggtt tttgtcttgt
     5641 agttcgtcga acagccggac gttctcaatg gcgatcaccg cctggcgtgc gaaagttgtc
     5701 aacagtgcaa tctgcttgtc cgtgaatggc cttacctcgg agcgacggat caagagagtg
     5761 ccaatggctt cgtcctccct tagcaaggga atgccgagag tggtccggtg accgaaacgg
     5821 agcgcgcgct cactgccatc ggggaactcg tcggcggcgg cccggaggtc atgcacgtgc
     5881 accggttcac gatcaacgaa ggctcgccca gtgacccagc cgcgatcaat cggcccctcg
     5941 cctcgcattg agattgggcc gtgatgcgct ctgccacaaa gccgctcgcc ctcgcgcagg
     6001 aaaataaccg aatcgtacgc ctcgcaaagc cggcaagaac tctcggcgat ggcttccaat
     6061 accggctgaa tattcgtcgg ggaggcagcg atcacaccga ggatctcgct tgtcacggtc
     6121 tgctgctcca gcgattcgcg cagctcgttg agcagccgcg tgttctcgat ggcgatgacg
     6181 gcctgggcgg cgaaattggt cagcaacgcg acttgcttgt cgctgaaggg acgaacctcc
     6241 tggtggtata tgacgatcgc accgatcagc tcattttctt ccttgaacat gggaacggcg
     6301 aggacgctgc ggtagccggc gagctccacg ttggcaacca cccgcgggtc acgctcgata
     6361 taggcgcgct gctgcacgag gtcggggata taggccacct ggcgtttggc ggcgatcaga
     6421 cccagcccgc tgtccgggtg cggccggaac acgccttcac tgcgtcgttg ctcaacccaa
     6481 gcagctgggg cgtcgtgctc cgcggtcagg cgaaaggtct cgccgttgcc gagaaacatc
     6541 gcagcgaact tcgcctggca gattcgcact gcgttttcca gcaaggcccg gtacaccgga
     6601 ccgagctccc ccggcgaact tgagatgacg cctaaaacct ctgatgtcgc agtctgccgc
     6661 tctagcgctt cgctcagatc acctgtgcgc tggcggagct cgttgagcag ccgcgtattc
     6721 tcgatggcga tcacggcctg ggctgcgaaa ttggtcaaca acgcgatttg cttgtcgctg
     6781 aagggaccaa cctcctggcg gtatatgaca atcgcaccga taagctcatt ttccttgaac
     6841 atgggaacgg cgaggacgct gcggtagccg gcgagctgca cgttggcaac cacccgcggg
     6901 tcacgctcga tataggcgcg ctgctgcacg
//
LOCUS       DATLJT010000660         1810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1878231,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000660 DATLJT010000000
VERSION     DATLJT010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878231"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1603
                     /locus_tag="VM910_15285"
     CDS             110..1603
                     /locus_tag="VM910_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVI63926.1"
                     /translation="MALMMPQSDAAVLERRAEIVAALKRIVPGEGVIDSEAAMRPFES
                     DGLTAYRQLPMVVVLPETTAQVAAVLGFCHREGIKVVPRGAGTSLSGGALPLGDGVLL
                     GLAKFNRIREIDYDNRVAVVEPGVTNLAVSQAVEAAGFYYAPDPSSQIACTIGGNVAE
                     NSGGVHCLKYGMTTNNVLGCELVLMTGEILRLGGKHLDSGGYDLMGVVTGSEGLLGVV
                     TEVTVRILKRPQTARAAIIGFAASEDGGECVAKIIGSGIIPGGMEMMDRPAIRATEQF
                     VHAGYPLDVEALLIVELDGPAAEVDHLLERVQEIARQCRATTCRISNSEAERLLFWAG
                     RKAAFPAVGRISPDYYCMDGTIPRAKLPLVLRRMQELSAKYGLRCANVFHAGDGNLHP
                     LILYDANKPGELERAEQFGSDILRICVEVGGVLTGEHGVGVEKRDLMPTMFSETDLNQ
                     QQRLKCAFDDKSLLNPGKVFPTLHRCAELGRMHVHAGRVAFPDLPRF"
BASE COUNT          298 a          566 c          596 g          350 t
ORIGIN      
        1 gccgacggct cctgcatgtc tgttctgtcg cactgacacg gcgcagccgg gtcgtgcgcc
       61 gccctgccgc atggcgctat aatgccggtt gcaacgcttg gaggatccga tggcgcttat
      121 gatgccgcag tccgatgccg ccgtccttga gcgccgcgct gagatcgtcg cagcgctcaa
      181 gcgcattgtg cccggcgagg gcgtgatcga ctccgaagcg gcgatgcggc cgttcgagag
      241 cgacggcctg accgcctatc gtcagctgcc aatggtcgtg gtactgccgg agaccaccgc
      301 gcaggtggcg gctgtgctcg gtttctgcca ccgcgagggc atcaaggtcg tgccgcgcgg
      361 cgccggcacc tcgctctcgg gcggcgcctt gccgctcggc gacggcgtgc tgctcggcct
      421 tgcgaagttc aaccgcattc gcgagatcga ctacgacaac cgcgtcgcgg tggtggagcc
      481 cggcgtcacc aatcttgcag tgtcgcaggc ggtggaggcc gccggctttt attacgcgcc
      541 cgatccgtcg tcgcagatcg cctgcaccat cggcggcaat gtggcggaaa attcgggcgg
      601 cgtgcattgc ctcaaatacg gcatgacgac caacaacgtg ctcggctgcg aattggtgct
      661 gatgaccggc gagatcctcc gcctcggcgg caagcatctc gattccggcg gctatgatct
      721 catgggtgtc gtcaccggct cggagggcct tctcggcgtg gtcaccgagg tgacggtgcg
      781 cattctcaaa cggccgcaga ccgcgcgcgc ggcgatcatc ggctttgccg cctccgagga
      841 tggcggcgaa tgcgtggcca agatcatcgg atcgggcatc attcccggcg gaatggagat
      901 gatggaccgt ccggcgatcc gcgccaccga acaattcgtg catgccggct atccgctcga
      961 cgtcgaggcc ttgctgatcg tcgagctcga cggcccggcg gccgaggtcg atcatttgct
     1021 ggaacgcgtc caggagatcg cccggcagtg ccgtgccacc acctgccgca tttccaattc
     1081 agaagcggag cggttgttgt tctgggccgg gcgcaaggcg gcctttccgg cggtcggccg
     1141 catctccccg gactattact gcatggacgg cacaataccg cgcgcgaaac tgccgctggt
     1201 gttgcggcgg atgcaggaac tgtcggcgaa atacggcttg cgctgcgcca acgtgttcca
     1261 cgccggcgac ggcaatctgc atccgcttat tctctatgac gccaacaagc ccggcgagct
     1321 cgagcgcgcc gagcaattcg ggtcggacat tttgcggata tgcgtcgagg ttggcggggt
     1381 tttgaccggc gagcacggcg tcggcgtcga gaagcgcgac ctgatgccga cgatgttttc
     1441 ggagaccgat ctcaatcagc agcagcggct caaatgcgca ttcgacgaca agagcctgct
     1501 caatcccggc aaggtgtttc cgacgctaca ccgctgcgcc gagcttgggc gcatgcacgt
     1561 gcatgctggg cgcgtcgcgt ttcccgatct gccgcggttt tgaaaatatt ttcgagtagg
     1621 gcaacgaata cgcgtcatga aattcggtct gttcacatcg gcgcaggcga acagcggcga
     1681 tctcggcccg gagaccggcc agggctttcg cgattatctc gacttcaatg tcgcggcgga
     1741 gcggctcggt ttcgtttcga gctttctggt cgaacatcat ttcaccggct ggaatcaggt
     1801 ctcggcgacg
//
LOCUS       DATLJT010000661         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2108101,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000661 DATLJT010000000
VERSION     DATLJT010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108101"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="proB"
                     /locus_tag="VM910_15290"
     CDS             complement(<1..1053)
                     /gene="proB"
                     /locus_tag="VM910_15290"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HVI63927.1"
                     /translation="MARRPQLKDFRRIVVKVGSSLLVDSKAGDVRGAWLSALAADIAG
                     LHKEGREILIVSSGSIALGRSRLKLPRGALKLEESQGAAAVGQIALARIWSEVLGAHG
                     IGAGQILVTLQDTEERRRYLNARSTIAKLLEWRAIPVINENDTVATNEIRYGDNDRLA
                     ARVATMATADLLILLSDVDGLYDAPPSQAPNARLIPVVERVTAEIEAVAGDAGSELSR
                     GGMRTKIEAAKIATTGGTHMLIASGKIEHPLQAIADGGRCTWFLTPANPVTARKRWIA
                     GSLEPKGTLTIDAGAVNALRAGKSLLPAGVIRVDGQFARGDAVVVRGPDTHEIGRGLV
                     AYDAEDADKIKGRSSPD"
     gene            complement(1152..1520)
                     /locus_tag="VM910_15295"
     CDS             complement(1152..1520)
                     /locus_tag="VM910_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI63928.1"
                     /translation="MIGYITVGTKDLKKAGEFYDKICGELGMGRFMANERLIAWGGRG
                     KGAGFGVALPYDGKPMTVGNGVMAAFGAKDKAQVDRVYKLALSLGGTCEGPPGQRSPQ
                     FYAAYFRDPDGNKLNAFVMG"
     gene            complement(1580..2653)
                     /gene="obgE"
                     /locus_tag="VM910_15300"
     CDS             complement(1580..2653)
                     /gene="obgE"
                     /locus_tag="VM910_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVI63929.1"
                     /translation="MKFLDEAKVYVRSGDGGNGCVAFRREKFIEFGGPSGGNGGRGGD
                     VIIEAVSGLNTLIDYRYQQHFKAQKGENGMGKDRHGANGKPIVLKVPVGTQVFDEDRE
                     TLIHDFTTLGEKFVLAEGGNGGFGNAHFKSATNRAPRNANPGQPGEERWIWLRLKLIA
                     DAGLVGLPNAGKSTFLAAVSAARPKIADYPFTTLHPQLGVVNADGREFVLADIPGLIE
                     GAHEGAGLGDRFLGHVERCRVLLHLVDATSEHAGKAYKTVRHELDAYGGSLNEKIEIV
                     ALNKIDAVDADELKKQRDRLKRAAKKTPLLVSGATGAGVREALRALADVIGEKPVTDK
                     AKVMAKSAADAEPWKDIPTSSQG"
BASE COUNT          426 a          865 c          862 g          519 t
ORIGIN      
        1 gtcgggcgag gagcggccct tgatcttgtc ggcgtcctcc gcgtcatagg cgacgagccc
       61 gcgaccgatc tcgtgggtat cggggccgcg caccacgacc gcgtcgccgc gggcgaactg
      121 tccgtcgacc cggatcacgc ccgccggaag caggctcttg cccgcgcgca gcgcgttcac
      181 cgcaccggcg tcgatggtca gcgtgccctt cggctcgagc gagccggcga tccatcgctt
      241 gcgcgcagtc accggatttg cgggcgtcag gaaccaggtg cagcgtccgc cgtcggcgat
      301 cgcctgcaag ggatgctcga tcttgccgga ggcgatcagc atgtgagtgc cgccggtggt
      361 cgcgattttc gcggcctcga tcttggtgcg catgccgccg cgcgacaatt cggatccggc
      421 atcgcctgcc accgcctcga tctcggcggt gacgcgctcg actacgggta tcaatctggc
      481 gttgggggcc tgcgacggcg gcgcgtcgta gaggccgtcg acgtcggaca acaggatcaa
      541 cagatcggca gtcgccatgg tggcgacgcg cgcggcgagg cgatcattgt cgccgtaacg
      601 gatttcgttc gttgcgaccg tgtcgttctc gttgatgacg gggatcgcac gccattccag
      661 gagctttgca atcgtcgagc gtgcgttgag gtaacggcgg cgttcttcgg tatcctgcaa
      721 ggtcacgaga atctgtcccg cgccgatgcc gtgagccccc agcacctcgg accagatgcg
      781 ggcgagggcg atctggccga ctgcggcggc gccctggctc tcctccagct tgagcgcgcc
      841 gcgcggcagt ttgaggcggc tgcggccgag cgcgatcgat cccgaggaga cgatcaggat
      901 ttcgcgacct tccttgtgca ggccagcgat atcggcggcc agcgccgaca gccacgcgcc
      961 gcgcacgtcg cccgccttcg aatcgaccag cagcgacgag ccgactttca cgacgatgcg
     1021 gcggaaatcc ttcagttgcg ggcggcgggc catgggcaga gttgcggtcg aaaagcggac
     1081 gtttgaaaca gcaatacgcg ccactccccg acttgggaag tggcgcgggc gtattgttcg
     1141 tgcagggcgt gtcagcccat tacaaaggcg ttgagcttgt tgccgtcggg atcgcggaaa
     1201 taggcggcgt aaaattgcgg gctgcgttgg cccggagggc cctcgcaggt gccgccgagc
     1261 gacagtgcaa gcttgtagac gcggtcgacc tgcgccttgt ccttggcgcc aaaggcagcc
     1321 atcacgccgt tgccgaccgt catcggcttg ccgtcatagg gcagcgccac gccgaacccc
     1381 gcgcccttgc cccgaccgcc ccaggcgatg aggcgctcat tggccatgaa gcggcccatc
     1441 ccgagctcgc cgcaaatctt gtcgtagaat tcgccggcct ttttcagatc cttggtgccg
     1501 accgtgatgt agccgatcat gaagtcctcc ctgtttcagg atagagggat tttcgcaagg
     1561 acgcggcctt caggcaagct cagccctgcg atgatgtcgg aatgtccttc cagggttcgg
     1621 cgtcggctgc gctctttgcc atgacctttg ccttgtcggt gaccggcttc tccccaatca
     1681 cgtcggcaag cgcgcgcagc gcctccctga ccccggcccc ggttgcgccc gacaccaaca
     1741 gcggcgtctt cttggcggcg cgtttcagcc ggtcccgctg ctttttcaat tcatctgcat
     1801 cgaccgcgtc gatcttgttg agtgcgacga tctcgatctt ctcattgaga cttccgccgt
     1861 aagcatcgag ctcatggcgc acggtcttgt atgcctttcc ggcatgctcc gatgtcgcat
     1921 ccacaagatg caacagcacg cggcagcgtt cgacatggcc gagaaaacgg tcgccgagcc
     1981 ctgcgccttc atgtgcgccc tcgatcaggc cgggaatatc ggcgagcacg aactcgcggc
     2041 cgtcggcatt caccacgccg agctgcggat gcagcgtggt gaagggatag tcggcgatct
     2101 tcgggcgcgc ggcgctgaca gcggccagaa atgtcgattt gccggcattg ggcagaccga
     2161 cgaggccggc gtccgcgatc agtttcagcc gcagccagat ccatcgttcc tcacccggct
     2221 ggcctggatt ggcgttgcgc ggcgcgcggt tggttgcgga tttgaaatgt gcgttgccga
     2281 acccgccatt gccgccttcg gcgagcacga atttctcccc gagcgtcgtg aaatcgtgga
     2341 tcaacgtttc cctgtcctcg tcaaatacct gcgtgccgac ggggactttg agcacgatgg
     2401 gcttgccatt ggcgccatgg cggtccttgc ccatgccgtt ctcgcccttt tgcgccttga
     2461 aatgctgctg atagcggtag tcgatcagcg tgttgaggcc ggagacggcc tcgatgatga
     2521 cgtcgccgcc gcgcccgcca ttgccgccgg aggggccgcc gaactcgatg aatttttcgc
     2581 gccggaacgc cacgcagcca ttgccgccgt cgccggaccg aacatagacc tttgcttcgt
     2641 cgagaaactt catggggtct tagctatgcc ag
//
LOCUS       DATLJT010000662         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2108115,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000662 DATLJT010000000
VERSION     DATLJT010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108115"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VM910_15305"
     CDS             complement(<1..918)
                     /locus_tag="VM910_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI63930.1"
                     /translation="MHAPAGRSPESSRFKPDSGQAWVRLGLALVIASIGAAGMWTVMV
                     VLPVVQAEFAATRGAVSLASTTMFMGFGLGAVITGRITDRFGIIAAMATSIALLGISL
                     ALAGLTVTLWQFIAVYFLIGLGTSATFAPLMAEVSHWFERYRGLAVAIVASGNYVAGT
                     IWPPLVNWGVQTIGWRSTHIALGAFCAVSMALLLLVLRARMGDEKIRDHSKAPPPQID
                     LRLSTNALTFLLSLASIACCVAMAMPQVHIVAYCGDLGYGVARGAEMLSLMTGFGIIS
                     RIGSGFLADRVGGIATLLIGSLAQAFALLF"
     gene            1292..>2757
                     /locus_tag="VM910_15310"
     CDS             1292..>2757
                     /locus_tag="VM910_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HVI63931.1"
                     /translation="MVDATAGQGASAPANFPKLSDIGSFIKRGDIALALGILTILVVL
                     ILPLPALVLDLFLAISITLSILILMTALFIQAPLEFSSFPTILLISTMLRLSLNLAST
                     RLILSRGHEGTDAAGHVIEAFGGFVMGGNFVIGIIVFAILVTVNFVVITKGSGRIAEV
                     AARFHLDSMPGKQMAIDADLSAGLIDEKVARERRKALEDESGFFGAMDGASKFVRGDA
                     VAGLLVVFINIIGGIIIGVAQQSLSFGEAAHTYTLLTVGDGLVTQVPALIVSTAAGLL
                     VSKAGVSGAADKALIKQFSVYPQALGMASAVMFVLALLPGIPTIPFLALAGGAAGLGW
                     TARKHKHAKAIAEAKAAAAPAATAASAAEEPIANALKIDDLKIELGYALLPLVNGPDG
                     TDRLTEQIKALRRSLAIEMGFVMPSVRILDNVQLEANTYVIKIKEVDAGSGKIWPNQF
                     MVMDPAGNQVNVPGIHTTEPTFGLPATWVDASLKEEASL"
BASE COUNT          522 a          930 c          858 g          447 t
ORIGIN      
        1 gaacagcagc gcaaaggcct gcgccagcga tccgatcagg agcgtcgcga tgccgccgac
       61 gcgatccgca agaaatcccg acccgatgcg gctgatgata ccgaaccccg tcatcagcga
      121 cagcatctcg gcgccgcgcg ccacgccata accgagatcg ccgcaatagg ccacgatatg
      181 gacttgcggc atcgccatcg ccacgcagca ggcgatggag gcaaggctca agaggaaagt
      241 caacgcgttg gtcgagagcc gcaaatcgat ctgcggcggc ggcgccttgc tgtgatcgcg
      301 gatcttctca tcgcccatcc gggcgcgcag taccagcaac aacagcgcca tcgacaccgc
      361 gcaaaaggcg ccgagcgcga tatgcgtgct gcgccagccg atggtctgta cgccccaatt
      421 caccaaggga ggccaaatcg tgcccgcgac ataattgcca ctcgcgacga tggcgaccgc
      481 caatccccga tagcgctcga accagtgcga gacctccgcc atcagcggcg caaacgtcgc
      541 cgacgtgccg agcccgatca ggaagtagac tgcgataaac tgccacagcg tgaccgtcag
      601 tccggccagc gcaagggaga tgccgaggag cgcgatgctg gtcgccatcg cggcgatgat
      661 gccgaagcgg tcggtgatcc gcccggtgat gaccgcgccg agcccaaaac ccatgaacat
      721 ggtggtggaa gcgagcgaca ccgccccgcg cgttgcggcg aactcggcct gcacgacggg
      781 cagcaccacc atgacggtcc acatgccggc ggcgccgatc gatgcgatca ccagggcaag
      841 accaagccgc acccaggcct gaccggagtc gggcttgaag cgtgaggatt cgggagatcg
      901 tccggcgggc gcgtgcacta ccggcaaact cctcgcccgc tgcccaaagg tcaacccgca
      961 atgccgctat attaggcatg cgcgcgcggc gcggcttcgt tccgaggcgc gaacgaagcc
     1021 ggttgcgcaa tcgcgcctcg gggaacccct cgcaacaact tcgagatttc ccgggttcgc
     1081 gcgaaagcga cccggaaggc aatccattaa ccctttccta accatacacc gggcaaaaat
     1141 tgccgggtga agtcgagtgt cgtcggccgc gtaaaacggg gagatctccg tggacgccag
     1201 tgcggtgcct cactttcgaa ccggtggccc atcagccgcc gcgcagacgc ttggggcccg
     1261 tggacggcat acgcgggggg acgcaggtta catggtcgac gccacggcgg gtcagggcgc
     1321 gagcgccccc gcaaacttcc cgaaactcag cgacatcggc agcttcatca agcgcggcga
     1381 catcgcgctt gccctcggta ttctcaccat tctggtcgtg ctgatcctgc ccctgcccgc
     1441 gctggtgctc gatctgtttc tcgcgatctc gatcacgctg tccatcctga tcctgatgac
     1501 cgcgctgttc atccaggcgc cgctcgaatt ctcgtcgttt ccgaccattc ttctgatctc
     1561 gaccatgctg cggctttcgc tcaacctcgc ctctacgcgg ttgatactgt cccgcggcca
     1621 tgaggggacg gatgccgccg gccacgtcat cgaagcgttc ggcggcttcg ttatgggcgg
     1681 caatttcgtg atcgggatca tcgtattcgc gatcctcgtc accgtgaact tcgtcgtcat
     1741 caccaagggt tcgggccgca tcgccgaagt cgcggcgcgc tttcacctcg actccatgcc
     1801 gggcaagcag atggcgatcg acgccgacct ctcggccggc ctgatcgacg agaaggtcgc
     1861 gcgcgagcgc cgcaaggcgc tggaggatga aagcggcttc ttcggcgcca tggacggtgc
     1921 ctcgaaattc gtgcgcggcg acgcggtcgc cgggctcctc gtcgtcttca tcaacatcat
     1981 cggcggcatc atcatcggcg tggcgcagca gagcctttcc ttcggcgaag cggcccacac
     2041 ctatacgttg ctcaccgtcg gcgacggtct ggtgacccag gtgcccgcgc tgatcgtctc
     2101 cacggcagcg ggcctgttgg tctcgaaggc gggcgtgtcg ggagccgccg acaaggcgct
     2161 gatcaagcaa ttctccgtct atccgcaggc gctcggcatg gcgtccgccg tgatgttcgt
     2221 gctcgcgctg ttgccgggca tcccgaccat ccctttcctt gcgctcgccg gcggcgcggc
     2281 agggcttggc tggacggcgc gcaagcacaa acacgcgaag gcgatcgccg aagcgaaggc
     2341 agccgccgcg cctgccgcaa cagcggcgag cgcagcggaa gagccgatcg cgaacgcgct
     2401 caagatcgac gatctcaaga tcgagcttgg ttatgcgctg ctgccgctgg tgaacggccc
     2461 cgacggcaca gaccgcctga ccgagcagat caaggcgctg cgccgttcgc ttgcgatcga
     2521 gatgggcttc gtgatgccgt cggtgcgcat cctcgacaac gtgcagctcg aggccaacac
     2581 ctatgtcatc aagatcaagg aggttgatgc cggcagcggc aagatctggc cgaaccagtt
     2641 catggtgatg gatcccgccg gcaaccaggt caacgtcccc ggcatccaca ccaccgagcc
     2701 gaccttcggc ctgcccgcga cctgggtcga cgccagcctg aaggaagagg catcgct
//
LOCUS       DATLJT010000663         6863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_728774,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000663 DATLJT010000000
VERSION     DATLJT010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728774"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VM910_15315"
     CDS             <1..614
                     /locus_tag="VM910_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI63932.1"
                     /translation="LLWVVFFMSLLDIYFLANWLPTVLNDLGASVSEAVLIGSLLQIG
                     GVAGTLALGSIIDRFSFLALALVYFGAVFAVGAIGQVSHSAVLVSIAIFGAGFCVVGG
                     QIAANALAANFYPTAVRATGVGWALGIGRVGSIIGPLVGGVLLAEKWSTAAVFMAAAA
                     AALCAALAALALSRVAMVSGGKGVAGQPSSLDATLKPAPTART"
     gene            629..793
                     /locus_tag="VM910_15320"
     CDS             629..793
                     /locus_tag="VM910_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63933.1"
                     /translation="MTSSDENKQRNREIAKNEANRQATSAVRVSTWAIAIVVFFGVIV
                     VSVVWAWLNR"
     gene            complement(796..1444)
                     /locus_tag="VM910_15325"
                     /pseudo
     CDS             complement(796..1444)
                     /locus_tag="VM910_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1456..2661)
                     /locus_tag="VM910_15330"
     CDS             complement(1456..2661)
                     /locus_tag="VM910_15330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI63934.1"
                     /translation="MDKGIFDGLKVLDCASFIAAPAAATVLSDFGADVIKVEPPGSGD
                     PYRNLPNLPGYPASEHNFAWLLESRNKRSLALDLSKPEGQAVLHRLAEQADVFITNYP
                     PAVRERLGITYDRLAPLNQRLIYASFTGYGEKGEEANKPGFDSSAYWARSSLMDLVRA
                     DTNTTPARSIAGMGDHPCAMAFYGAIVTALYKRERTGKGSHVASNLMANGVWAASVLA
                     QAKLCGAKFGERRPRERALNALTNHYKCKDGRWMILSVLNEERQWPALARCLEREDLI
                     ADPRFATKPDRHARSVELIKLFDDVFATKTLAEWRKLLDGSGLVFGVVGILDDIPTDR
                     QMLDNEVLVPFENDTMLTINSPIWVSGSKKAPPRKPPGIGEHSDEILRDAGYDEASIE
                     KLRSSGAVA"
     gene            complement(2771..5785)
                     /gene="putA"
                     /locus_tag="VM910_15335"
     CDS             complement(2771..5785)
                     /gene="putA"
                     /locus_tag="VM910_15335"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HVI63935.1"
                     /translation="MTSASPASLSLPPFAAAYAPEDNAFAARLLNTARLSAEQERRID
                     RVATRLIEAIRASDDRLGGVEDMLREFALSTKEGLALMVLAEALLRVPDARTADQFIE
                     DKLAEGDFIHHETKSSAFLVNASAWALGVSARVIQPGETPEGTIGRLAKRLGAPAVRA
                     ATRQAMRLMGSHFVLGETIESALARANGATASRYSFDMLGEGARTAADAERYFRSYRS
                     AIEAIGRTVDGRALPDRPGISVKLSALHPRFEAISRGRVMGELVPRLIELAGLAKAYD
                     LNFTVDAEEADRLELSLDVIASAFSDSSLGGWDGFGLAIQAYQKRACDVIDYVDALAR
                     TLDRRLMVRLVKGAYWDTEIKRAQERGLEGYPVFTRKAMTDLNYVDCAKKLLALRGRI
                     FPQFATHNALTVATVLELAGNEGGFEFQRLHGMGDALYARLRQDRPDLAYRTYAPVGS
                     HRDLLAYLVRRLLENGANTSFVALAADDAIPARTLLRRPADMIGSAGQARHPNIPLPR
                     DLYMPERKNSRGIEFGERAALDALVSAIAAETTPAKGSVADATPADAARAVALVRAGF
                     LRWSATPAEARAEILERAADLLEDRGAHFMALLQREGGKTLDDALSEVREAADFCRYY
                     AAQGRRLFGAGEAMPGPTGESNQLRLRGRGVFVAISPWNFPLAIFLGQVTAALMAGNA
                     VVAKPAEQTPLVAAAAISLLHEAGVPASALHLVPGDGAIGAALVAHPDIAGVVFTGST
                     EVARAINRVLAAKDGPIIPLIAETGGINAMIADATALPEQVADDVVTSAFRSAGQRCS
                     ALRLLFVQDDVADRMIEMIAGAARELKIGDVSDPATHIGPVIDAEAKARLDAHIARMT
                     AQARVHFAGTAPAGNFVAPHIFELSDAGQLTEEVFGPILHVVRYGADRLRQVLQSIEA
                     TGYGLTLGVHSRIDDMVEAIVARLAVGNIYVNRNMIGAVVGVQPFGGQGLSGTGPKAG
                     GPHYLVRFATEQTVTVNTAAAGGNAALLSGEE"
     gene            complement(5916..6116)
                     /locus_tag="VM910_15340"
     CDS             complement(5916..6116)
                     /locus_tag="VM910_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVI63936.1"
                     /translation="MAKGTVKWFNPTKGYGFIQPSSGGKDVFVHISAVQKAGLSTLNE
                     GQTIEYEEVANRGKTSAENLKV"
     gene            complement(6469..>6863)
                     /locus_tag="VM910_15345"
     CDS             complement(6469..>6863)
                     /locus_tag="VM910_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI63937.1"
                     /translation="FDGVRRFSEFQKSLGLSKGILSARLRRLVALGVLKPAPASDGSA
                     YQEYVLTQKGLDLFPVVVALRQWGEDHCFARGEQHSVLVDNETGHRIARLEVRSKSGR
                     ALRSSQTLVRKLGRPSPPTRRSARPAGR"
BASE COUNT         1190 a         2176 c         2208 g         1289 t
ORIGIN      
        1 ccttgctgtg ggtggtgttc ttcatgagcc tgctcgatat ctattttctg gcgaactggc
       61 tgccgacggt gctcaacgat ctcggcgcgt cggtctcgga agccgtgctg atcggctcgc
      121 tgctgcagat cggtggcgta gccggtactt tggcgcttgg aagcatcatc gaccgcttct
      181 cgttcctggc gttggcgctg gtctatttcg gcgccgtttt cgcggtcggc gcgatcggcc
      241 aggtcagcca ctccgcggtc ctggtatcga ttgcgatctt tggtgccggc ttctgcgtgg
      301 tcggcggcca gatcgccgcc aacgcgcttg ccgcgaactt ctatccgacc gcagtgcggg
      361 cgaccggtgt cggttgggcg ctcggcatcg gccgcgtcgg ttcgatcatc ggcccgctgg
      421 ttggcggggt gctgctggcc gagaaatgga gcacggcggc ggtcttcatg gcggcggctg
      481 cggcggccct ctgcgcggcg ctggcagcgc tggcgctgag ccgtgttgcc atggtcagcg
      541 gcggcaaggg ggtagccggc cagccgtcat cattggatgc tacactcaag ccggcaccga
      601 cagcgaggac ttgagtggga atcggccgat gacctcttcc gacgagaaca agcagcgcaa
      661 tcgcgagatt gcgaagaacg aggccaaccg acaagcgacc agcgccgtcc gcgtcagcac
      721 ttgggccatt gccattgtcg tctttttcgg cgtgatcgtc gtctcggtcg tctgggcctg
      781 gctcaatcgc tgaggttaga gcgtcgaggc gtagtatttc agccgcttgc cgggcaagag
      841 atcgtaggcc gacttccagc cgggaagcga ggccatacga ttgagccagg cgtggatggc
      901 gggatggctc gtggcgaaat cgtaaccggt ctcgctcttg gggtagtgta gataggccat
      961 catggaaaag tcggcgacgg tcagccgatc gccgatcgcg aaggcatttt ggaccagatg
     1021 ctgctcgagc acggagagga aatcgtcgag acgccgccga aaatatgtca gcaccttcgg
     1081 atcgggcgat ggcgtgaacg cacgctgata gcggtaggtc gccatatagc cggtgagctt
     1141 gtggttgtcc cagaacagcc atcggagcag ttcaaactgc tccttttcgg tcctggtacc
     1201 gaaccggcca tactgcttcg ccaacatcag cagaatcggt gccgtctggg tcagacgttc
     1261 gccatccact tcgagcaccg gaatttcgcc catcgggttg acggcgcgcc gccactcggc
     1321 cgtgcgggtg gtgccgccgc cgaaatcggt ccagacgggc tcgaagctct ccccgcacag
     1381 ggtcagcata cagcttgtag ctgttgccgg actccgggaa gtagtgcagg cgatacttgg
     1441 gcatctttgt cgtattcacg ccaccgcgcc ggatgaacgc aatttttcga tcgacgcctc
     1501 gtcgtagccc gcatcgcgca ggatttcgtc ggagtgctcg ccgatgccgg gcggctttcg
     1561 cggcggcgcc tttttcgagc cgctgaccca gatcgggctg ttgatcgtga gcatggtatc
     1621 gttctcgaac ggcaccagca cctcattgtc cagcatctgc cggtcggtcg gaatatcgtc
     1681 gaggattccg accacgccga acacgaggcc actgccgtca agcagcttgc gccattcggc
     1741 cagagttttg gttgcgaaga cgtcatcgaa cagcttgatc agctcgacgg agcgggcgtg
     1801 acggtccggc ttcgtcgcaa acctcgggtc tgcgatcaaa tcctcccgtt cgagacagcg
     1861 cgcgagcgcc ggccattgcc gttcctcgtt cagaaccgac aggatcatcc agcggccatc
     1921 cttgcatttg tagtgattgg tgagcgcgtt cagcgcgcgc tcgcgcgggc gccgctcgcc
     1981 gaatttcgcg ccgcagagtt ttgcctgcgc cagcacggag gccgcccaca cgccgttcgc
     2041 catcaggttc gaggcgacat gcgagccctt gccggtgcgc tcgcgcttgt agagcgccgt
     2101 gacgatcgct ccataaaacg ccatggcgca gggatgatca cccatgccgg cgatcgagcg
     2161 cgccggtgtc gtgttggtgt ccgcgcgcac gaggtccatc aggctcgatc gcgcccagta
     2221 ggcgctgctg tcgaagcccg gcttgttggc ttcctcgccc ttttcgccgt agccggtgaa
     2281 ggaggcgtag atcagccgct ggttcagcgg cgcgaggcga tcgtaggtga tgcccagccg
     2341 ttcgcgtacg gccggcggat aattggtgat gaaaacatcg gcttgctccg cgagcctgtg
     2401 cagcaccgcc tgcccctccg gtttcgacag atcgagcgcc aggctccgct tgttccggga
     2461 ctccagcagc cacgcaaaat tgtgctcgct tgccggatat cccggtaggt tcggcaggtt
     2521 gcgatagggg tcgccggatc caggtggttc gaccttgatc acgtcggcgc cgaagtccga
     2581 cagcactgtc gcagccgcgg gtgctgcgat aaagctcgcg cagtcgagaa ccttcaggcc
     2641 gtcgaagatg cctttgtcca tcgtggcgtc gctcccgtcg ttatgttgct tgcagcccgt
     2701 cggaacttcc ctggcagccg atatccccgt catttgaccg atgtcgggcc gggatgcaac
     2761 ggcgatggcg tcattcctct cctgacagga gcgcagcatt gccgccggcc gcggccgtgt
     2821 tgacggtcac ggtctgctcg gtcgcaaagc gcacgagata gtgtggccca cccgccttcg
     2881 gtcccgtgcc cgacaggcct tgaccgccga acggctgcac gccgaccacc gcgccgatca
     2941 tgttgcgatt gacatagata ttgccgacgg caagtcgcgc cacgattgct tcgaccatat
     3001 cgtcgatgcg cgagtggacg ccaagcgtaa gcccataacc ggttgcctcg atcgactgca
     3061 gcacctgtcg gagccggtcg gcgccataac gcaccacgtg caggatcggc ccgaacactt
     3121 cttcagtcag ttgaccggca tccgagagct caaagatatg cggggcgacg aaatttccgg
     3181 cgggtgcggt gccggcgaaa tgcaccctcg cctgcgccgt catgcgggcg atgtgggcat
     3241 cgagccgcgc cttggcttcg gcatcgatca ccgggccgat atgggttgcg ggatcgctca
     3301 cgtcgccgat cttcagttcg cgcgcggcgc ccgcgatcat ctcgatcatg cgatcggcga
     3361 cgtcatcctg aacgaacagc agccgcagcg ccgaacagcg ctggcctgcc gagcgaaacg
     3421 ccgaagtcac gacatcatcg gccacctgct cgggcagcgc ggtcgcatcg gcgatcatcg
     3481 cgttgatgcc gccggtttcc gcgatcaacg gaatgatcgg gccatccttg gcggcgagaa
     3541 cccggttgat cgcgcgcgca acttccgtcg aaccggtaaa gaccacgccg gcgatgtcgg
     3601 ggtgtgcgac cagcgcagcc ccgatcgctc cgtcgcccgg cacgagatgc agcgcggaag
     3661 ccggaacgcc ggcttcgtgc aacagcgaaa tcgcagcggc cgcaaccagt ggggtctgtt
     3721 cggcgggctt ggcgaccaca gcattgccgg ccatcagcgc tgcggtgacc tggcccagaa
     3781 agatcgcgag cggaaaattc catggcgaga tcgcgacgaa gacgccgcgg ccgcgcagcc
     3841 ggagctgatt gctctcgccg gtcggtcccg gcatcgcctc gcccgcgccg aacagccttc
     3901 ggccctgggc cgcgtagtac cggcagaagt cggccgcttc gcgcacttcc gacagcgcat
     3961 cgtcgagcgt cttgccgccc tcgcgctgca acagcgccat gaaatgcgcg ccgcgatctt
     4021 cgagaagatc ggctgccctt tcgaggatct ctgcgcgcgc ctccgcgggc gttgcactcc
     4081 agcgaaggaa gccggcgcgt accagggcga ccgcgcgcgc ggcgtcggct ggtgtcgcgt
     4141 cggcaacgct gcctttcgcg ggcgtagtct cggccgcgat ggctgagacc agcgcgtcca
     4201 gcgccgcgcg ttcgccgaac tcgatcccgc gtgaattctt ccgctccggc atgtacagat
     4261 cgcgcggcaa tggaatattc ggatgacgcg cctggccggc gctgccgatc atatcagccg
     4321 ggcgccgcaa cagggtcctt gccgggatcg cgtcgtcggc ggcaagagca acgaacgagg
     4381 tgttggcgcc gttctcgagc aggcgccgca cgagataggc gaggaggtcg cgatgactgc
     4441 cgaccggcgc ataggtccgg tacgccagat cggggcggtc ctgccgcaag cgcgcataca
     4501 gcgcgtcgcc catgccgtgc agccgctgga actcgaagcc gccctcgttg cccgcaagct
     4561 cgagcacggt cgcgaccgtc agcgcgttgt gggttgcgaa ttgcggaaaa atccggccgc
     4621 gcagcgccag aagttttttc gcgcaatcga cataattgag gtcggtcatc gccttgcgcg
     4681 taaacacggg atatccctca agcccgcgtt cctgcgcgcg cttgatctcg gtgtcccaat
     4741 aagcgccctt gaccaggcga accatcaggc gccggtcgag cgtccgcgcg agtgcgtcga
     4801 cataatcgat cacgtcgcat gcgcgcttct ggtaggcctg gatcgcaagt ccaaagccgt
     4861 cccatcctcc aagcgaactg tcgctgaaag ccgaggcaat cacgtcgagc gacagctcca
     4921 gccgatccgc ttcctcggca tcgacggtga aattcaggtc ataggctttg gcgagtccgg
     4981 caagttcgat cagtcgaggc accagttcgc ccatcacgcg gccgcggctt atcgcctcga
     5041 accgcggatg gagcgccgat agcttgaccg aaatgccggg acggtcgggc agggcgcggc
     5101 catccacggt gcggccgatc gcttcgatcg cgctccggta agatctgaaa taacgttcgg
     5161 catcggcagc ggtccgcgcg ccctcgccga gcatgtcaaa ggaatagcgc gacgcggtcg
     5221 ccccatttgc gcgggcgagc gcggattcga tggtttcgcc cagtacgaag tggctcccca
     5281 tcaaccgcat cgcctgccgc gtggcggcgc gaacggcggg tgcgccgagc cgctttgcca
     5341 gccggccaat tgtgccctcg ggtgtctcgc cgggctggat tacgcgcgcc gagacgccca
     5401 atgcccaggc cgacgcgttc accaggaacg cgctcgactt ggtctcgtga tggatgaaat
     5461 cgccctcggc gagcttgtcc tcgatgaact ggtcggcggt gcgggcgtcc ggcacgcgca
     5521 gcagtgcttc cgcaagcacc atcagcgcga gaccctcctt ggtcgagagc gcgaactcgc
     5581 gcagcatgtc ttcgacgccg ccgagccggt cgtcgctggc gcgaatggcc tcgatcagac
     5641 gcgtcgcgac gcgatcgatg cgccgctcct gttcggcgga gagccgcgcg gtgttgagaa
     5701 gtcttgccgc aaacgcgtta tcctccggcg cgtaagccgc ggcgaagggg ggcagggaca
     5761 gggaagcggg ggaagcggac gtcatggcgt gcctaatggt ccgggcactt taccacgcaa
     5821 cctgttggcg cgaaaaagcc gcctgacgtg cggggcggaa gcccgccccc gccgaaatat
     5881 gattaactca aatgaggaag tgcgcgtgcg ccgcgctaga ccttgaggtt ctcagccgaa
     5941 gtcttgccgc gattggcgac ctcttcatat tcaatcgttt gtccttcgtt gagggtggaa
     6001 agtcctgcct tctgcaccgc cgagatatga acgaatacgt ccttgccgcc cgaggagggc
     6061 tggatgaatc cgtagccctt ggtcgggttg aaccacttga ccgtaccttt agccatacgt
     6121 cgtctccgcg ggatcaataa attaataaag ggcgaaccgc cgatccccgc aaaccggctt
     6181 gttcagccca acagaatacg ctggttagcg caaccttgat agttctaacc agtggccgga
     6241 tttcgccgga aatgctcgat atcgtggcgc atttaaggat tttgcctgtt tcgcgatcat
     6301 tttgaggcgc ccgcatcacg aaattcccgc tgtgccatgc gaatcgcgca agggcgcccg
     6361 gcccgccggg gcggatggac gagggcattt gtccagtcga gttctgaggc tcggcatttt
     6421 cacgggatgg acgacgtcgc tcaggtcagc caaattcgcc ctataccctc aacgaccggc
     6481 cgggcgtgcg cttcgtctgg ttggtggtga tggcctgccg agttttctta cgagtgtttg
     6541 ggaagaccgc agggctcggc cgcttttcga ccgcacttcc agccgggcga tgcggtggcc
     6601 ggtttcattg tccacaagca ccgaatgctg ctcgccgcga gcaaagcagt ggtcctcgcc
     6661 ccattgccgc agggccacca cgacgggaaa caaatccagt cccttctggg tgaggacgta
     6721 ttcctggtag gcgctaccgt ccgaggccgg cgcaggcttc agcacgccaa gcgcgaccaa
     6781 tctcctgagg cgagcgctaa ggatgccctt cgataaaccc aacgacttct gaaactcgct
     6841 aaatcggcga acgccatcga agg
//
LOCUS       DATLJT010000664        19007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2337959,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000664 DATLJT010000000
VERSION     DATLJT010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19007
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337959"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VM910_15350"
     CDS             <1..1360
                     /locus_tag="VM910_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63938.1"
                     /translation="DWKVSDDKLTYTFTLRDGLKWHDGQPVTAEDCVASLKRWAARDG
                     MGQKLMDFTASLEATDAKTITLKLNESYAYVLESIGKPSSLPAFMMPKRLAETPPDKS
                     IPEQIGSGPFKFVTAEFQPGIKSVYVKNPDYVPRSEPPDWTSGGKVVKVDRVEWITMA
                     DPQTALNALQAGDIDFVERPPWDMLSILENDRRLVVHTLDAAGFQTFGRMNFLHPPFN
                     NLKMRRAALLALRQKDVLDALTGNPKYYRLCGAVFGCDTPLATDVGSEVLVKGNGMAE
                     ARKLLAESGYDGTPVAIMAPGDVLAVKSQPIVAAQLLRAAGFKVDVLAMDWQTLVTRR
                     TSQKPPKEGGWNMFFSNLTGQDIANPITHLQINGKGTNGAWFGWPRDSNMEELRDAFA
                     RAKSLEEQKEIATEIQARVYDQVIYIPLGQWTIPSVWRREISGVLDGPATPVFWNVEK
                     TD"
     gene            complement(2277..2519)
                     /locus_tag="VM910_15355"
     CDS             complement(2277..2519)
                     /locus_tag="VM910_15355"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HVI63939.1"
                     /translation="MRHPTIIALAAFTLLLVDATSSAIAARYPLQGCHWGYSNNCRFS
                     SHRQCVQDNIWAELLCLCTPASAPTLYGVRRAAHRK"
     gene            complement(3817..5343)
                     /locus_tag="VM910_15360"
     CDS             complement(3817..5343)
                     /locus_tag="VM910_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI63940.1"
                     /translation="MKFVSTLATWPVVALLAGAPCALAGQAPGSAADPDIPVTHADRV
                     YAAEQFSNTVSVTDPADNKLLGLIRLGEPSPVNFSPLYKGQLLVHGMGFSPDHRTVAV
                     VSIGSNSVTFIDTATNTVKHTTYVGRSPHEAFFTPDGREVWVTVRGENYVSVLDGADF
                     QERTRIVVANGPGMTIFSPDGKYGYVCSSFTPEIDVVTVADHAVVAKVPQASPFCPNI
                     AATPDGAQIWFTLKDTGKTQVFDARPPFSILKTLDTGPITNHVNIVRNSRGQFAYVTV
                     GGLNEVQVFRTDDFSKVATIKVGKLPHGIWPSGDGTRIYVGLENDDQLIAIDTLSNSV
                     VASVPIGQAAQAIVYVPGAVPPTGNSGNLAMTDMSGAPNTVTESMGTRMLQPLSVAGQ
                     AARFTLQPVGDKPAAGAPPTNVTLFDQGLIQVLEASVTGLEPMHPYVLALAAGKDGSG
                     ALEPLTNFMTNPAGAAIVNALGPIRQIVQGEVELPRRYLVIVQGSAAEPGKPVQVQAP
                     "
     gene            complement(5358..5666)
                     /locus_tag="VM910_15365"
     CDS             complement(5358..5666)
                     /locus_tag="VM910_15365"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HVI63941.1"
                     /translation="MFLAENASAMSKMMINMGIRPSGNIDADFARIMIAHHQGAIEMG
                     LAELRHGHDERLMRLAQEIVITQQQEIAVMRLALGQLADAPRQSPVSEADNKREFAND
                     "
     gene            6334..6567
                     /locus_tag="VM910_15370"
     CDS             6334..6567
                     /locus_tag="VM910_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63942.1"
                     /translation="MSDEHPELDELQAAYKNTVEAWIAAIRKEEALASVNHEVADVDK
                     WEQAHLEEDELRNKVKTAKEEYEDALRAKFFGF"
     gene            7217..8410
                     /locus_tag="VM910_15375"
     CDS             7217..8410
                     /locus_tag="VM910_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63943.1"
                     /translation="MLGLAVGATPAAAQKKYDPGASDTEIKVGNIMPYSGPLSAYAQI
                     GRTEAAFFRMINEQGGINGRKINFISYDDAFSPPKTVEQARKLVESDEVLLIFQSLGT
                     PTNNAIQKYMDQKKVPQLFVATGATKFGDPKNFPWTMGWQPTYQTEGRIYAKYILQNF
                     PNGKIGILYQNDDSGRDYLKGLKDGLGPEATKRMIVAELPYDPSDPTVDSQVVALKTI
                     GADIFFDEASPKFAAQAIRKAAEIGWKPTLFLASISNSVGSVLKPAGLENSKGILSSN
                     YIKDPTDPTWKDDPAIKQWSEFMDKYFPEGDKSSTFTVYGYALSQTLVQVLKQCGDEL
                     TRKNVMKQATNLKDFEVGLLLPGIKINTSPTDYYPIEQMQMSRFNGEHSVLFGTAIFG
                     EIGTQ"
     gene            8567..8944
                     /locus_tag="VM910_15380"
     CDS             8567..8944
                     /locus_tag="VM910_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63944.1"
                     /translation="MLTWILIPGACAWFALLLLILHKMAKNGDEAIPKKKWWSRDRRV
                     SKTAEELESTITEAVKAAPGCEAFVGVIVQRTTPKSRLDANWELRGIKFGRVDRRIAK
                     EVLTPIVERMQREFRLLPRLSTA"
     gene            9455..9676
                     /locus_tag="VM910_15385"
     CDS             9455..9676
                     /locus_tag="VM910_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63945.1"
                     /translation="MPTQIVMNRNGDTRHYFDANDAAALAKAEQRFKKLTGEGFTAAV
                     RIKPGEVARTNSFDPNAEETLFYPRLVGG"
     gene            9687..10088
                     /locus_tag="VM910_15390"
     CDS             9687..10088
                     /locus_tag="VM910_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63946.1"
                     /translation="MFGFCRIPIGRRTRLKAVRRLYISSNDENNPQARGLRLLHAWLS
                     PPQLEQLDAKGHFDVIGSATGRRYRIHFGTSMNVHELDDAGRLKMGWCFVPDGCLVPG
                     DVMLAQKIALETFESRALSVAIKFVPNERLC"
     gene            complement(10467..10679)
                     /locus_tag="VM910_15395"
     CDS             complement(10467..10679)
                     /locus_tag="VM910_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVI63947.1"
                     /translation="MPESIYDVILLIGTSSESWEKAAANAVEQASKSLRNLRVAEIVE
                     LDMQLDAKGKVEAYRAKVRLSFKVDR"
     gene            complement(11309..11569)
                     /locus_tag="VM910_15400"
     CDS             complement(11309..11569)
                     /locus_tag="VM910_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63948.1"
                     /translation="MPRKGITGHDEWVVVEALATAFVALEQLPEKHQPRAHMEDIRKI
                     LSSGREPATVSLHLAQAKCRLFPDRDPLAIYQEYGISDSYYG"
     gene            complement(11667..12641)
                     /locus_tag="VM910_15405"
     CDS             complement(11667..12641)
                     /locus_tag="VM910_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI63949.1"
                     /translation="MRRRDFIGLICGALGPSFVTRTASAEAAYPSRPVRIIAGFAPGG
                     GVDITARLVGQWLGDRLGQPFVTENRTGADGNIATEAVVNAAPDGYTLLLATLPNAVN
                     ASLYPKLNFVFLRDIAPVAGVIRVPMVVLVHPSLPAATIAELIAYGKANPGKINMASA
                     GTGSAPHMAGELFSSMAGISMLHVPYRGQSPALSDLLGGQVQVLFAAAPGTVDYIRSG
                     KLRALAVTSATQADVLKDLPTIASSLPGYEASQWYGIAAPRRIPADIVARLNSEINAA
                     FDDAAMKSRFAAISGEPLSGSPEEFGRLMSDETEKWAKVVQFAGLKIE"
     gene            12807..14039
                     /locus_tag="VM910_15410"
     CDS             12807..14039
                     /locus_tag="VM910_15410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVI63950.1"
                     /translation="MKSAIVLGAGMVGVTTALHLQRRGWSVALVDRKPPGQETSYGNA
                     GIIQSEAPKPYPMPRDWLSLARIAMGRTNDVRYELSALPHHLGPLLRYWWHSLPRRHE
                     KISAAYARIIAQSAGEHDELIRESGAGNLVQRDGYRVLHRDQAAFDADVASAESLRAA
                     FGVKFSVLTPQDLAQAEPGLMETGLGGLHWRDPWTVSNPGALVTAYAALFERLGGTIL
                     LGDAETLARGGRGWSVAGSAGKIDAEAAVIALGPWSPESLKRFGYRFPMVRKRGYHRH
                     YCGGSMLHLPLRDVANGYVMAPMTNGVRITTGAELSAPDAGLTPVQLGSAEAAARKLI
                     DLGSPVEAEPWLGTRPCMVDMLPVLGPAAGQPGLWMNFGHGHQGFTLGPVTARIVAEM
                     MSGEAPAVDPRAYRPDRF"
     gene            14239..14640
                     /locus_tag="VM910_15415"
     CDS             14239..14640
                     /locus_tag="VM910_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63951.1"
                     /translation="MRFLSFVYRFITNFAFLAAVYVSLNYIDKYNNRAILAILVLIYA
                     GMRAASALHSFYFFQRIERLEVEARRVIALLTEGGAQSPLKKQTVADVAMLRRDGEIK
                     SYIDLFFLAAVVLLCVAKIVTDQGSTPERQP"
     gene            complement(14739..15587)
                     /locus_tag="VM910_15420"
     CDS             complement(14739..15587)
                     /locus_tag="VM910_15420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HVI63952.1"
                     /translation="MIEYVATASEIARYTGCQRNLEAQLDNFFIAKDFDWQVRRSSNL
                     DRALNRATRRVGINSDTAGLVDQMIYKLTGYKLAAARSGISTCIEQRMNMYHLVSQTL
                     AYQVEGDLVEVGCNAGQSAVLIAKVMRDFASDKELHVYDSFEGLPPTKVEDGTVYKEG
                     DLAVSEETVRKNFLLHDLKIPAIHKGWFEDTLPATLPAKVSFAHLDGDLYDSIMVSLK
                     YVYPRLTRGAICLVDDYCDPQINPDGWNYLPGVKKACDEFLSDKPERICYPYSGVFTH
                     AFFRKL"
     gene            complement(15651..16979)
                     /locus_tag="VM910_15425"
     CDS             complement(15651..16979)
                     /locus_tag="VM910_15425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI63953.1"
                     /translation="MISNRSVDVRIGSWAVFLLALACGPMAQAADCPGHPDAIGTSRT
                     LVVDPSAHPRLGTMQYRETLPLRDHEVVLTFDDGPLPKNSNQVLQTLADNCVKATFFT
                     IGEQARANPEGVRKLAAAGHSIGTHSQTHPLTFHKMNLQQAQKQIDDGIDSTLAAVDD
                     PSDLAPFFRFPGLLRSDVAEGYLASKGIQVWSTDLLADDWHHISSAGVYDLAVKRLEA
                     KGKGILLLHDIQARTAAALPKILQTLKARGFHIVHVVPATPEQPATPTEPVDWQLYPP
                     SEAVPIARWPKVPSFTIAEAEGFVAPALSDFDTPDGKLLLSAEPFAHVKRQGGVRLPT
                     EASWPRAVDLPIELAAVLPVPDQTLFELPKDADAAFRSPPPHGASVAESEMEGYDKKA
                     SAGKKRGSRVAHGAGRSGRLAHAAQKPGKHPAHANHGAKRPVRVASLKKR"
     gene            complement(17074..17826)
                     /locus_tag="VM910_15430"
     CDS             complement(17074..17826)
                     /locus_tag="VM910_15430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI63954.1"
                     /translation="MSLGCVAATHAADCPRKGVLGTSRVLAVDPAKFPRVGLKSFPQT
                     LPLRDHEVVLTFDDGPWPPTTPKVLAELAQECVHATFFLIGKPASEHPDLVRRIAAEG
                     HTIGHHTWLHRSLMRIRPSETTDEIEHGISAVELALHGVATTTPSTPFFRFPGFESTP
                     ATLNFLQAQGIAVFGADLWASDWIRLTPQQELKLLIKRLKVSNRGIILLHDPKAQTAA
                     MLPAFLRYLRDNDYHVVHLVPKQPGVDSEAGP"
     gene            17974..>19007
                     /locus_tag="VM910_15435"
     CDS             17974..>19007
                     /locus_tag="VM910_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI63955.1"
                     /translation="MAEHMDVAPSADATVLDRFPMRGEDGEIRPEFVEEIADRIDAGD
                     ADVLRQAVAELHEADLGDLIAALAPEARVRLVELTGTDFDFSALNEVDGAVREEILEE
                     LEPETVAEGVRELESDDAVELLEGLDEKDQEEILEKLPPQERDALERSLDYPENSAGR
                     RMQTEFIAVPPGWTVAQAIDYMRETPDLPARFYEIYAVDGAKRWQGAIPLDVLLRSRR
                     SVPLADLIDAERHRVSVTDDLEEVARLFGKYNLVAAPVVDGNDRLVGVITVDDVVDVI
                     EEEADEEIKALGGVTSDEELSDNFWTIAKGRFNWLLVNLATAFLASFVLGLFEGQLER
                     MVALAVLAPIV"
BASE COUNT         3781 a         5668 c         5832 g         3726 t
ORIGIN      
        1 ggactggaag gtgtccgacg acaagctcac ctacacgttc accctgcgcg acggcctgaa
       61 atggcacgac ggccagccgg tcacggcgga ggattgcgtg gcttcgctga agcgctgggc
      121 tgcgcgcgac ggcatgggcc agaagctgat ggactttacg gcgagcctgg aagcgacgga
      181 tgcaaagacc atcacgttga agctgaacga gtcctatgcg tacgtgctgg aatcgatcgg
      241 caaaccctcg tcgctgcccg ccttcatgat gccgaaacga ctggccgaaa cgcccccgga
      301 caagtcgatc ccggagcaga tcggctcggg tccgttcaaa ttcgtgactg ccgaatttca
      361 gccgggcata aagtcggttt acgtgaagaa tcctgactat gtgccgcgca gtgaaccgcc
      421 ggattggacc tcgggcggca aggtcgtgaa ggtcgatcgg gtcgaatgga tcaccatggc
      481 cgatccgcag actgcgctga acgcgctaca ggccggtgac atcgatttcg tcgagcggcc
      541 gccgtgggac atgctgtcga ttctggagaa cgaccggaga ctcgtcgtgc acaccctcga
      601 cgctgccggc tttcagacgt tcgggcgcat gaacttcctt caccctccct tcaacaacct
      661 gaaaatgcgg cgggcggcgc tgctggcttt gcggcagaag gatgtgctcg acgctctcac
      721 aggcaatcca aaatattaca ggctctgtgg agccgtgttc ggctgcgata ctcctttggc
      781 caccgacgtc ggatcggaag tcctggtgaa gggcaatggc atggcggagg cgagaaagct
      841 gctcgcggaa tccggttatg atggcacacc tgtcgcgatc atggcgccgg gggacgtatt
      901 agcggtgaaa tcacagccga tcgtcgcggc ccagctcctg cgcgcggccg gtttcaaggt
      961 ggacgtgctc gcgatggact ggcagacctt ggtcacgcgt cgcaccagcc aaaagccgcc
     1021 gaaggagggc ggctggaaca tgttcttttc caacctgacg ggacaagata tcgccaaccc
     1081 gattacccat cttcaaatca acggcaaagg tacgaacggc gcctggttcg gctggccgcg
     1141 ggactccaat atggaagaat tgcgtgacgc ctttgcgcga gcgaagtccc tggaggagca
     1201 gaaggagatc gcgaccgaga tccaggcgcg cgtttacgac caggtgatct acattccgct
     1261 cgggcagtgg accataccat ccgtctggcg cagggagatc agcggtgtac tcgacggccc
     1321 cgccacacca gtattctgga acgtggagaa aacggactga aatagactcg ccgctgcgaa
     1381 atccttgaga cagagtcgcc cgcctttttg gtcccgtgcc gaaccaggcg ccgaccgccg
     1441 caacgtcggc tgtcgaggct aaagcgcgat gagatcaggt tgatattgct gcgccggagg
     1501 tgcgctcgct cccccgcttg cgggagaggg ttgggggttg gggcatgaac gacgggcact
     1561 ttaggtacgt ttgcccccac ccgccgcact ctttgagctc cgcaatccgg ccgcacgtcg
     1621 gcgccgcgct cgcgtgtaag aacctggcgg aagccgatgc cttctcactt cgcggggata
     1681 gcgacaggtg gcggaggtga ggcataggcg gctggcggcg gaggcgcata gttaggacaa
     1741 acctggacag gctgcccggt gtaccgacga cccggccgta gggatcgtaa gccgcttggg
     1801 atgtccagta gcacccgggg ccgtaaagcg gctgagcata accgggatac acgacatagc
     1861 ctgcggggcg tgaggccacg attgcgccgg caattatcgc gccgccgacg accccggccg
     1921 caacccctgg cccccatccc cagccgcgtc gccagtacgc cgacgcatct gttgtggtcg
     1981 cggcgaaagt gagcccgatt gttgcactca tcagcaaaac agcgagcatc cttttcatca
     2041 gcttctcctt taacgtatcc accaattata gcgggccaag gcgcagaggc agcgaaggca
     2101 tctccgttca ctatgccaac aagctgttgc tccgtattaa gtcaaaaccg cggtacccta
     2161 cccaattgaa acaagattct gtcgctcatt cgagattaat tcgctgtaat gttttgcgtg
     2221 caggccggat tcacatgctg catgagttgc cagatgctgc agcaccgagc cttttctcat
     2281 ttacggtggg ctgctcggcg caccccgtac agggttggcg ccgacgctgg cgtgcaaagg
     2341 cataatagtt cggcccagat attgtcttgc acgcattggc gatgcgagga gaaccggcaa
     2401 ttattggaat agccccaatg gcaaccctgc agcgggtagc gcgcggcgat ggccgatgat
     2461 gttgcatcga ccagcagcag agtgaaggcg gcaagcgcaa tgatcgtagg atgccgcatg
     2521 cttctgctcc gcttcgagct ttcaacaacg tgctccacaa tcgtgctctc gcgcgacgga
     2581 tactggttgg tccagccgta gccccgctca ccgctcgttt cgcatgtagg cgctatgagc
     2641 ggtgatcagt ttgagatcag gatcgtcata aagaacaggc tgcggcttca gcttgtaatt
     2701 ggacagtgcg tccaaggcgg cctcctggcg cctctcggcg tgggagatca tcagcatagc
     2761 agcaacgcaa gccaagctca cgcaggccag caatatcctc aaggcgccct cctttcggaa
     2821 gatcatgatg aatcaagcgg gcaacgcttc tgatcacgcc gctgcgctat cttccttgtc
     2881 cgcgccccgc attgagcgcg tcgtccggca gtagccgctt gctcccctat gccgctaagg
     2941 caaaaaaccg aaacccggct gccgcgagcc tatcgggaga taactcttta accgtctccg
     3001 actctgcaaa cagaaagcag ctcggacagt tgaacagatg cagattggtg accgggacaa
     3061 atctaacgag ctccgaccga caccaccgca tctcgatgcc gcaggtcggg caggtcggca
     3121 cttcacgatc ttccagcgag tgcttcagtc tgtccatcac aaccctcccc aggtcgagtg
     3181 atgaaagccc caagcccaac aggcaaagcc tttacccaca ggttcgacgt gtcatcagct
     3241 accttgttca cggctttttc caggcgtctt ctgggcgtct ctcggactaa ctctccgtat
     3301 gttccgggga ggaggagggg gaactcaagc aggtggcgag aaggcaatcg ctgcctatag
     3361 cagcgcggct tacggtgaag cgctcaaggc accagacgac ggagactcgg cctcgaatgg
     3421 cgccgcgagt atcccgtccg gacgacgtac cggcaggcgc ctcagatcgt gacgatattg
     3481 ttgtttgcaa cttgcaactt cgagcgcgtt cacgcgacca gcggccgtgc cgctatccgt
     3541 gaacttcacg ttacctaatc agaagctgaa aaccagcaca gcggcgagtg ccgccgatac
     3601 tgtgccgagt gtgattgcaa aaaaaatgaa tgccccatcg ctcatgggtc ctagtgtagc
     3661 gccgttctgc gacggttgct tgctccagtt ggggcggccc ttgcgaatgc aatttatttc
     3721 tctttgagaa aggcgcggca gaggtgttct tctagaaggg ttcgatcgat tctgtgtccg
     3781 cttcggtcca tagccgacac tttcgggcgc cagtatctac ggcgcctgga cctggacagg
     3841 ctttccaggc tccgcggctg agccctgcac gatcaccaga tagcgccgcg gcaactcgac
     3901 ctcgccctgc acgatctggc ggatcggacc gagcgcgttc acgatcgctg cacccgccgg
     3961 gttggtcatg aaattggtca acggctccag cgcaccggag ccgtccttgc cggcggcgag
     4021 cgcgagcaca taaggatgca tcggttcaag gcctgtgacg gaagcttcaa gcacctggat
     4081 cagcccctga tcgaacagcg tgacattggt cggcggcgcg cccgccgctg gcttgtcgcc
     4141 taccggctga agcgtgaaac gcgccgcttg gccggcgacg ctgagtggct gcagcatcct
     4201 cgtgcccatg ctctcggtga cggtattggg agcgccggac atatccgtca tcgccagatt
     4261 tccgctattg ccggtgggag gcacagcgcc gggcacatag acgatggctt gtgcggcctg
     4321 accgatcgga accgacgcga cgacgctgtt ggagagcgtg tcgatcgcga tcaactggtc
     4381 gtcgttctcg agaccaacgt aaatgcgcgt tccgtcaccg gaaggccaga tgccatgcgg
     4441 cagcttgccg accttgatgg tcgcgacctt gctgaaatcg tcggtacgaa acacctgcac
     4501 ttcgttgagg ccgcccaccg tgacgtaggc gaattggccg cgggagttgc gcacgatgtt
     4561 gacatggttg gtgatcgggc cggtgtcgag ggtcttgagg atcgagaacg gcggcctggc
     4621 gtcgaagacc tgcgtcttgc cggtatcctt cagcgtgaac cagatctgcg cgccatcggg
     4681 cgtcgcggca atgttgggac agaacgggct cgcttgcggt actttggcga cgacggcatg
     4741 atcggcgacg gtgacgacgt cgatctccgg cgtgaacgac gagcagacgt agccgtattt
     4801 gccatcgggc gaaaagatcg tcatgcccgg accgttggcg acgacaatgc gtgtcctttc
     4861 ctggaagtcg gcgccgtcga gcaccgacac ataattttcg ccacgcaccg tgacccacac
     4921 ttccctgccg tcgggcgtga aaaaggcttc atgcggcgac cgtcccacat atgtcgtgtg
     4981 cttcacggtg ttggtcgccg tatcgataaa ggtaacggag ttggagccga ttgacacgac
     5041 tgcgacggtg cgatgatcgg gggaaaaacc catgccgtgc accagaagct gccccttgta
     5101 gagcgggctg aaattgacgg gcgatggctc gcccagcctg atcaagccca agagcttgtt
     5161 gtcggcggga tcggtgaccg acaccgtgtt ggaaaactgt tcggcggcgt agacgcgatc
     5221 agcgtgagtg accggaatgt ccggatcggc ggcactgccg ggcgcctggc cagccagggc
     5281 gcatggcgcg ccggcaagga gcgcgacaac aggccaggtg gcgagtgtgg aaacgaattt
     5341 catctcaccc tcccaagtta atcgttggca aattcacgct tgttgtccgc ctcggacaca
     5401 ggcgattgcc tgggcgcgtc cgcaagctgg ccgagcgcga gccgcatcac ggcgatttcc
     5461 tgctgctggg tgatgacgat ctcctgcgca agacgcatga gcctctcgtc atggccatgg
     5521 cgcagctccg caagtcccat ctcgatcgcg ccttgatgat gcgcgatcat gatgcgcgcg
     5581 aaatccgcat cgatgtttcc agagggccgg atacccatgt tgatcatcat cttcgacatc
     5641 gcgcttgcgt tctcggcaag gaacaaggac tcatccggcg aagacgaccg caatggcgcc
     5701 aggcagatcg cggacagata ttcggcaagc gagcgcggcg ccttagaaca ggcggccaat
     5761 gagacggcaa tcgcaatcaa cccgcaggcg aaacgaaccc gctttgaata aaatcgggaa
     5821 cagcggagac gggacatcgt gcgtttcatt tcaacaccct ttgccgttgt gccccggtga
     5881 ccgcgaattt tggcacactg ccatgccgct gaccacaaac ttgatagtat cttcagcaag
     5941 acgccggcgc tgcggcgcgc gtcgagcttc gagaaggcgt gcgacgcttc gaacccgcgt
     6001 ggcaaaggat cggctcactg gaaagctcgt cgctgcacgg agctatccct gatttgatcg
     6061 cgctggcgcg ggcagacaac taattggcgc accgaaacaa gaagctgcgg ttgacgcgcg
     6121 gcgatcggga ttgcaaacac gcgcccgagg cggatgagcc tcgcgtcaac ttcagcggtc
     6181 ggcccgcgcg gccggtcgct ttttttgatc gaagcgcggc gctcgccgcg agcatctgga
     6241 tcgtcgtccc gacaccgcag cgatgtcgtc atttgttcat tcgcactctc tataacgatg
     6301 gttattgaga taaacctttg gtcgggggtc gaaatgagcg atgagcatcc cgagcttgac
     6361 gaactgcagg cggcctacaa aaacacggta gaagcgtgga tagcagccat tcgcaaggag
     6421 gaagcactcg cttccgtcaa tcacgaggtg gccgacgtcg acaagtggga acaggcgcat
     6481 ctcgaagagg atgaactgcg caacaaggtc aagaccgcca aggaagaata cgaagacgcc
     6541 cttcgcgcca agttcttcgg gttctaacgc agaggccccg tgtggccctt tcctccgcct
     6601 ttgcgtcgat gggcggaaac cataagggca tgcccgccgc taaggctcaa cgaaggtcaa
     6661 ccgcctccga aggtggatgg gtcaagattt ctgttcgcct ccggcgggat gggcggatag
     6721 ccaggaaact tgtaccagcc cggggggacg gccgtacctt ggctgcccgg aacggcggcg
     6781 aagcttgagg ccacgcctgg gcgaatgatg acatgatgcg tgcgggagcg atgcgccgtt
     6841 gcagcatcag ccgaagcaca gaagatcatc aagaggccca aaaccaaaac ggaacgcatc
     6901 gcattgctcc aaccgattgc cgctgctcct tatgtgatcg gcggaccgcg ccgccccaat
     6961 ctggggcggc ttcacaattg ttcacatcga cgggatcatg gctcatgctc accgacccga
     7021 tcctcgtatc gcgctggttt cagagccagt ttggcaccag tgccacctcc tggaaaaata
     7081 gaggcgacag ggtgaagctc tgacttcatt ggctggactt cgctcgctct caacccctag
     7141 actgccccgg agatcggcaa tcagccggcg gctatccagg ggaggatgaa atgacgcaaa
     7201 ggcacgttct gctggcgtgt tgggtcttgc ggttggtgcc acacctgccg ccgcacagaa
     7261 gaaatatgac cccggcgcgt cggacaccga gatcaaggtc ggcaacatca tgccctatag
     7321 cggtccgctc tcggcttatg cccagatcgg ccggaccgaa gcggcgtttt tcaggatgat
     7381 caacgagcag ggcggcatca acgggcgcaa gatcaacttc atctcctatg acgacgcctt
     7441 cagcccgccc aagacggtgg agcaggcgcg caagctcgtg gaaagcgacg aggtgctctt
     7501 gatcttccag agccttggca cgccgaccaa taatgcgatc cagaaataca tggatcaaaa
     7561 gaaggtgccg cagctctttg tcgcgaccgg cgccaccaag ttcggcgacc ccaagaattt
     7621 cccatggacc atgggttggc agccgaccta ccagaccgag gggcggatct acgcaaaata
     7681 catcttgcag aacttcccga acgggaagat cggcattctc taccaaaacg acgattccgg
     7741 ccgggactat ctcaagggcc tgaaagacgg gcttgggcct gaggccacca agcgcatgat
     7801 cgtcgccgag ctgccctatg atccttcgga cccaacggtc gattcccagg tcgtggcctt
     7861 gaagaccatc ggcgccgata tcttctttga cgaggcctcg ccgaaatttg cggcgcaagc
     7921 gatccgcaag gctgccgaga tcggatggaa gccgacgctg ttcctggcca gcatatccaa
     7981 ttcggtgggt tcggtgctca aacccgcggg gctcgaaaat tccaagggga tactctcgtc
     8041 caactacatc aaggatccga ctgatccgac ctggaaggat gaccccgcca tcaagcagtg
     8101 gtcggagttc atggataaat atttcccgga aggggacaag agctcgacct tcacggtcta
     8161 cggatacgcg ttatcacaaa cgctggtaca ggtgctcaag caatgcggcg acgagctgac
     8221 gcgcaagaat gtcatgaagc aggccaccaa cctgaaagat tttgaggttg gcttgctctt
     8281 gccgggcatc aagatcaaca ccagtccgac cgactattat ccgatcgagc agatgcagat
     8341 gtcacgcttc aacggcgaac acagcgtgct gttcggaacg gcgatcttcg gcgagatcgg
     8401 cacgcagtag ccggagcatg atgaggtgag gtcgttactc cctccctgaa agggaagggg
     8461 gaagagaagt ctcggcgctt agatcgcgcc gtgctggacc tcgtggaaca aaaatccagt
     8521 cgcctgcata acagggcatg ccttccgaag gcgaaggagc agagcgatgt tgacctggat
     8581 cttgatcccg ggcgcttgcg cttggtttgc gctgctgttg ctgatcttgc ataaaatggc
     8641 caaaaacggc gatgaagcca tcccgaagaa gaaatggtgg agcagggaca ggcgggtttc
     8701 aaaaacagcc gaagagctgg aatcgacgat cacggaagcg gtcaaggctg cgcctggctg
     8761 cgaagccttc gtgggtgtga ttgtccagcg cacgacaccg aaatcacgcc tggatgcaaa
     8821 ttgggagctt cgggggataa agtttggtcg cgtggatcga aggatagcca aggaagttct
     8881 gacgcccatc gtcgagcgca tgcaacggga atttcgtctt ctcccaaggt tgtcaacggc
     8941 ttaaggccgc cgttattttg cctgtttggg cgcgatcctc aatactgtca cgatgagttg
     9001 tcaaattgag gccgcggcgg aattgcgggg atgggaacga tcgcccaagc gaactgttga
     9061 aagggcgcgt tctccgggaa cgccgaaggg gcccaggcgg ctccagctcg ctactcactc
     9121 gctggagccg gtttcgttcg aatgcaaatc gtggatgcgg gacatgcctg ctgttctggt
     9181 gatggttatt ctgtcaggcc tgacgcttgg caccgtcggg gcagattatt tcaagttccg
     9241 cgcggtactt gaaccggcat cggagacgcc tgcacagcag agcgcgcggg ccagccgaaa
     9301 gtggtgcccc ccaaattctt ccgaaaaggc actcgaacga cctctgcccg gtgacgcggg
     9361 atcgtgatgc gcggcgaggc agaagtgaat ggaactccgg tcacgcagga acatttcttg
     9421 gtagaactgg cggcaacagc cgaggagcat tttcatgccc acgcagatcg tcatgaaccg
     9481 caacggtgat acccgccact acttcgacgc caacgatgca gcggcgctgg ccaaagcaga
     9541 acagagattc aaaaagctga cgggcgaagg ttttaccgct gccgtccgca tcaagcctgg
     9601 cgaagtcgcc aggaccaact cgttcgatcc caacgccgag gaaacgctat tctacccgcg
     9661 gctcgtcggc ggctagaggt ctgtcgatgt tcggattttg tcgaattccg atcggtaggc
     9721 gaacgcgcct caaggccgta cgcagacttt acatcagctc caatgatgaa aataacccgc
     9781 aggctcgtgg tctgcgcctt ttgcacgcgt ggctttcgcc gcctcaactc gagcagttgg
     9841 atgccaaggg ccactttgat gtcatcggta gcgccaccgg taggagatac cgaatccact
     9901 tcgggacgtc gatgaacgtc cacgagctgg atgatgcggg tcgtctcaaa atggggtggt
     9961 gcttcgtgcc cgatggttgc ctagtccccg gcgacgtgat gctcgcacaa aagatcgctt
    10021 tggagacatt cgagagccgc gccttgtcgg ttgccattaa gttcgtgccg aacgaaagac
    10081 tgtgctgaac tcgcaggcgg atatgttcca ggagcgatat cggttcccgg tgttgcagaa
    10141 tggactccgg catccatttc ttgtggacta acccaccatt aacgcccgcg ccgttaccat
    10201 gctgctaaag aaccgcttgg gcatggggag atctcaacat ggcggatata acgcagggcg
    10261 tgcttttcat cttggcgtgt gcattcctgt tcggcctgac gctgtttctg tttcgtgatc
    10321 aaatcacgag caggcgttga tccgtggatt ctggcgggac accctgtcgg caaatcgaga
    10381 tcgctgagtc acgatgtggt gacccaccgc aagtccaaaa acctattcgt gaacaactcg
    10441 gcagcttgac gcgccgcgat atcggttcag cgatcgacct tgaacgacag cctgactttc
    10501 gcgcgatagg cttccacctt tcccttcgcg tccagttgca tgtcgagttc gacaatttct
    10561 gcgacgcgaa gattgcggag agatttggag gcttgttcga ccgcgttggc ggcggccttt
    10621 tcccaggatt cgctgctggt gccgatcagc agtatcacat cgtagatgct ctcgggcatg
    10681 ttgacctccc gttcggtatg gaagcagggt actccccgcc gcctcgcagc ctaacatcac
    10741 gaagcatgcg atgtcgcctg cgacattcgt cctctggcca tcgcattctg gagtgggacc
    10801 gggtccggcc tggcctgcct cgcgattttt caacgagtgc cgtgccctgc catcgcttgc
    10861 ttcgagacgt aagctcaccc cggttggaac gaacaactgt cacagaagta ctctaccctt
    10921 gagtgaatga gtaacggagc attcgcgttt tttgcaatca cacttggcac aatttcggta
    10981 gcattggccg ccctgctggt tttcggcttt taaagggccg cctcattggg ggcccttttg
    11041 attttgcggg cctttgattt ggatcaacag tctggcttgc cgaggagcag agcctggctt
    11101 cgatgcctct tatgaggcat cactcaatgc tcattctgac tgacttgagg ccgcctaggg
    11161 cgcgggcggc cctctttcaa gccagaaccc ggcgtcacgt gtcagggtgt tgcgtatggc
    11221 gataccgtgg agtcgatgag agcacgcccg ggtcgacaca tcgattcata tgacgtgcct
    11281 ggtgcttcag cctgacgcgg ctggtgcttc agccgtaata actgtcgctg atgccgtatt
    11341 cctggtagat cgcaagcggg tcgcggtcgg gaaacaggcg gcacttggct tgcgcgaggt
    11401 gcaggctgac ggtcgccggc tcgcgtccgc tggacaagat cttcctgata tcctccatgt
    11461 gggctctcgg ctggtgcttt tccggaagct gctccagcgc gacgaaagct gtcgctaatg
    11521 cctcgacgac aacccactcg tcgtggcccg tgattccctt cctgggcatc ggctcactcc
    11581 aggtccttta actctactag aggcccttta actctgtagc attgtatctc gaacgtggac
    11641 cgaccgccat cgagcgcttc gtcggcctat tcgatcttga ggccggcgaa ctgcacaact
    11701 ttcgcccatt tttcagtctc atccgacatc agtctgccga actcctccgg gcttccggag
    11761 agcggctcgc cggaaatggc ggcaaaacgc gacttcatcg cggcgtcgtc gaaggcggcg
    11821 ttgatctcac tgttcagccg cgcgacgatg tcggcgggta ttcttctggg cgcagcgatg
    11881 ccgtaccact ggctcgcctc gtagccgggc agggaactgg caatcgtcgg caggtccttc
    11941 agcacgtcgg cctgcgtcgc cgaggtgacc gcgagcgcac gaagcttgcc gcttctgatg
    12001 tagtccacag ttcccggcgc agccgcgaac aggacctgca cctggccgcc caagagatcc
    12061 gatagcgccg ggctctggcc gcgatagggc acgtgaagca tgctgatgcc agccatggac
    12121 gaaaacagct cccccgccat atgcggcgcg ctgccggtgc cggcggaagc catgttgatc
    12181 ttgccgggat tggctttgcc ataggcgatc agctcggcga tcgtcgcggc cggcagagag
    12241 ggatgcacca gcaccaccat cggcacgcgg atcacgccgg ccacgggggc gatgtcgcgc
    12301 aggaacacga aattcagctt cgggtacagt gaggcgttga cggcattcgg gagggtggca
    12361 agcagcagcg tataaccgtc gggtgccgcg ttcacgaccg cctcggtcgc aatatttcca
    12421 tcggcgccgg tgcggttttc agtaacgaat ggctggccga gccggtcgcc aagccactgg
    12481 ccgacgagtc gcgcggtgat gtcgacgccg ccgcccggag cgaagccggc gatgatgcgc
    12541 actggccggc tgggataggc ggcttccgcc gacgctgttc gggtgacgaa cgatggcccc
    12601 agcgcgccgc agatcagtcc gatgaaatct cgtcgtctca tgaacgcccc gagttcagtc
    12661 tcgaataagg taacggtcgg cggctcggca aggaaggcct tgcgcgagcc cgatgcaatc
    12721 gcccttagca agggggacag cgtcatgtcc ccattgttga cgcacctgtt gccttgcagc
    12781 cgccgtgttg taagtctgct ctcgtcatga agagcgctat cgtcctcgga gctggaatgg
    12841 tcggcgtgac gaccgcgcta catctgcagc gccgcggctg gtcggttgcg ctcgtcgatc
    12901 gcaagccgcc agggcaggag acgagttacg gcaacgccgg catcattcaa agcgaggcgc
    12961 cgaaacccta tccgatgccg cgcgattggc tgtccctcgc aaggattgcc atgggccgca
    13021 ccaacgacgt gcgctacgaa ctctccgcgc tgccgcatca tcttggcccg ttgctgcgct
    13081 actggtggca ctcgttgccc cggcggcacg aaaaaatttc agcggcctac gcacggatca
    13141 tcgcgcagtc cgccggcgag catgatgagc tgatccgcga atccggcgcc ggcaatctgg
    13201 tgcaacgcga cgggtaccgg gtgctccacc gcgatcaggc ggcgttcgac gccgatgttg
    13261 cctcggccga atcgctgcga gcggccttcg gcgtgaagtt cagcgtgctg actccccagg
    13321 atcttgcgca ggccgaaccg ggcctgatgg aaaccggtct tggcggtctt cattggcgcg
    13381 acccctggac cgtctcgaat cccggcgcgc tcgtgacagc ctatgcggcg ctgttcgagc
    13441 ggctgggcgg aacgatcctt cttggcgacg ccgaaacgct tgctaggggt gggcgcggct
    13501 ggtcggtggc cggcagcgcc ggaaagatcg atgccgaagc ggccgtgatc gccctgggtc
    13561 cgtggtcacc ggagtctctg aaacggttcg gctaccgttt cccgatggtg cgcaagcgcg
    13621 gctatcacag gcactattgc ggcggaagca tgctgcatct gccgctgagg gacgtcgcga
    13681 acggctatgt catggcgccg atgaccaacg gcgttcgcat caccaccggc gccgaattgt
    13741 cggcgcccga tgcgggcctg accccggttc agctcggctc cgcggaagct gcggcgcgca
    13801 agctgatcga tctcggttcg ccggtcgagg ccgaaccctg gctcgggacg cggccctgca
    13861 tggtcgacat gcttccggtc ctcggtcccg ccgccgggca acccggactc tggatgaatt
    13921 ttggacacgg acaccaggga tttacgttgg gaccggtgac ggcccggatc gtcgccgaaa
    13981 tgatgagcgg cgaggcgccg gccgtcgatc cgcgggccta tcgacccgat cgcttctgac
    14041 actccccggt cggcgcgccc gcgtaaccgg gcgttaatct tcccgaggcc cgtgccgcca
    14101 gccgcgtcgg gcgcatccgt taccttcccg ttagaacatt cgcgtacaca gcaggcgtga
    14161 aagtcacctt ggcgtttgtg gcacccttgc tgcgtccgca aatgtctgaa aatacgtctc
    14221 tcgacacagg cacaccgcat gagattcctg agcttcgtct accgcttcat caccaacttt
    14281 gcgtttcttg cggcggtcta tgtcagtctc aactacatcg acaagtacaa caatcgcgcc
    14341 atcctcgcga tcctggtcct gatctatgcc ggcatgcgcg cagcgtccgc gttgcactcg
    14401 ttctattttt tccagcgcat cgaaaggctc gaggtcgagg cgcgccgggt gatcgcgctg
    14461 ctcacggaag gtggcgcgca atcgcctttg aaaaagcaga ccgtcgccga cgtcgcgatg
    14521 ctgcgtcgcg acggcgagat caaatcctac atcgatctgt tctttctggc ggcggtcgtc
    14581 ctgctctgcg tcgccaagat cgtgacggat caggggtcaa ccccggaacg gcaaccatga
    14641 agaaagctcg ttgcggtggc cgatgccgcg gcgcgtaatc gggatgcggc cggtgcctgc
    14701 caacgagacg accggcctta tcggcgtcga acccggcttc aaagttttct aaaaaatgcg
    14761 tgcgtgaaga cgccggaata gggatagcaa atccgttctg gcttatccga caagaactcg
    14821 tcgcacgcct tcttgacgcc gggcaaataa ttccatccgt ccggattgat ttgcggatcg
    14881 cagtagtcat caacgagaca gatcgcgccg cgcgtcaggc gcggatagac gtattttagg
    14941 ctaaccatga tggaatcgta gagatcgccg tccaaatgcg cgaacgagac tttggctgga
    15001 agcgttgccg gaagcgtatc ctcgaaccag cctttatgta tggccggtat tttcagatca
    15061 tgcagcagaa agttcttgcg caccgtctct tcggatacgg cgagatctcc ttctttgtac
    15121 acggtgccat cttcgacctt ggtaggtggc aggccctcaa agctatcgta aacgtggagc
    15181 tccttatcgg aggcgaaatc gcgcatcact ttggcaatga ggaccgcaga ctgcccggca
    15241 ttgcaaccca cctcgaccaa atcaccttcc acttgataag cgagtgtctg ggaaacgaga
    15301 tggtacatat tcattcgttg ctcgatgcac gtcgatattc cggagcgtgc agccgcaagt
    15361 ttgtagcccg tcaatttgta tatcatttga tcaacgagcc cggcggtatc cgagttgatt
    15421 ccaacacggc gggtcgcgcg gtttaacgct ctatccaggt ttgacgagcg tcggacctgc
    15481 cagtcgaaat ctttcgctat gaagaaattg tccaattgcg cctccaggtt tcgctgacat
    15541 cctgtatacc gcgcgatttc gctggcggtc gcgacatatt cgatcacctc atcagcaccg
    15601 gcgggttgcg gatacggtag cccgagcttt tgcgggcgcc gaagcgccgc ctagcgtttt
    15661 ttcaagctcg ccacccgcac cgggcgcttt gcgccgtgat tggcgtgcgc cggatgcttt
    15721 cccggttttt gcgctgcatg cgcgagccgc ccgcttcgcc cagcgccatg tgcaacacgc
    15781 gagccccgct ttttcccggc cgacgccttt ttgtcgtagc cctccatctc gctctcggcc
    15841 accgacgcac catgcggcgg gggcgatcgg aaggctgcgt ctgcgtcctt tggcagttcg
    15901 aacagggtct gatcgggcac gggcagaacg gcggccaact cgatcggcag gtcgaccgcg
    15961 cgcggccatg atgcttccgt cggcagccgc acgccgccct gacgtttcac atgtgcaaac
    16021 ggctccgctg aaagcagcag cttgccatcc ggcgtgtcga aatccgacag cgccggggcg
    16081 acaaaccctt cggcctcggc aatggtgaag ctcggaacct tcggccagcg cgcgatcggc
    16141 accgcctcgg acggcggata caattgccaa tccacaggct ccgttggtgt tgccggctgc
    16201 tcaggcgtcg ccggcacgac gtgcacgatg tgaaatccgc gggctttcaa cgtctgcagg
    16261 atcttgggca gcgccgccgc ggtacgcgcc tggatgtcgt gcagcaaaag gatgcccttg
    16321 ccctttgctt cgagccgctt gacggcgagg tcgtagacgc ccgccgacga aatgtggtgc
    16381 caatcgtcgg cgagcagatc ggtactccag acctggatgc ccttcgaggc gagatatccc
    16441 tcagcgacgt cgctgcgtag caagcccgga aagcggaaga acggcgccag gtcggacgga
    16501 tcgtccacag ccgccagtgt cgagtcaatg ccgtcgtcga tctgtttctg cgcctgctgg
    16561 agattcatct tgtgaaaggt gagcggatgc gtctggctgt gcgtgccgat cgaatgaccg
    16621 gcggccgcca gcttccgcac gccttcagga ttggcgcgcg cctgctcgcc gatggtgaaa
    16681 aaggtcgcct tgacgcaatt gtcggccagc gtctgcagca cctggttgct gttcttcggc
    16741 agcggaccgt cgtcgaaggt cagtaccacc tcgtgatccc tgagcggcag cgtctcccga
    16801 tattgcatgg tgccgaggcg cggatgggcg ctcggatcga cgacaagagt tcgtgaggtg
    16861 ccgatcgcgt cgggatggcc gggacagtcg gccgcttggg ccatggggcc gcacgcaagt
    16921 gccagtaaaa agaccgccca cgacccgatc cgaacatcga ccgaacgatt actgatcatg
    16981 gggcaccgtc tgtaatcccc gcatcaaagc cgattattac ctgctgcgcc atgaacgcag
    17041 cattaactga tcggtttagc gacatgtttc agatcaggga cctgcctcgg agtcaacgcc
    17101 cggttgcttc ggaaccagat gaaccacgtg atagtcgttg tcccgcaaat aacgcaggaa
    17161 tgccggcagc atggccgccg tctgggcctt ggggtcgtga agcaggatga tgccccggtt
    17221 cgacaccttg agccgcttga tcaggagttt cagttcctgt tgcggcgtca atctgatcca
    17281 gtcgctggcc cagagatcgg cgccaaaaac cgctatcccc tgcgcttgca ggaaattgag
    17341 ggttgcgggg gtcgactcga agccgggaaa ccggaagaac ggcgtgctcg gggtcgtggt
    17401 cgcgaccccg tgcaatgcca gttcgaccgc cgaaatgccg tgctcgattt catcagtggt
    17461 ctcgctcggc ctgatccgca tcaggctgcg gtgcaaccag gtgtggtgcc cgatggtgtg
    17521 gccctcggct gcgattcgcc gcaccaggtc cggatgttcg gaggcgggtt tgccgatcag
    17581 gaaaaacgtg gcgtgcacgc attcctgggc gagctccgca agcaccttcg gcgttgtcgg
    17641 cggccacggg ccatcgtcga acgtcagcac cacctcgtga tccctgagcg gcagggtctg
    17701 cggaaaactc ttcaggccca cgcgcggaaa ctttgcggga tcgacggcaa ggacgcgcga
    17761 ggtgccgagc acgcccttgc gcggacagtc ggccgcatgc gtcgccgcga cacatcctaa
    17821 cgacatcaga agcgcggctg ccgttgagcg gatcggcgtt gcgaccggtt ttgctccaga
    17881 catctttggc attgcgcttc ccaggctatt gagccatgcc tctcaagaca gctcagacga
    17941 cttcaaccat tctgtcaaac gggaaggcgc gccatggccg aacatatgga cgttgccccc
    18001 tcggctgatg cgaccgtact cgaccgcttc cccatgcgtg gggaagatgg cgagattcgt
    18061 cccgaattcg tcgaggagat cgcggaccgc atcgatgccg gcgatgctga tgttctgcgc
    18121 caggccgtgg cggaactgca tgaggccgac ctcggcgatt tgattgcggc gcttgcgccg
    18181 gaggcgcgcg ttcgcctggt cgaactgacc ggaaccgatt tcgatttctc cgcgctcaac
    18241 gaggtcgacg gtgccgtccg cgaggaaatc ctcgaagagc tcgagccgga aacggtcgcg
    18301 gagggcgtgc gcgagctcga atctgacgat gcggtcgaat tgctcgaggg gctcgacgaa
    18361 aaggatcagg aagagatcct ggagaagctg ccgccgcagg agcgcgatgc gctcgaacga
    18421 agcctcgact atcccgagaa ttccgccggc cggcggatgc agaccgaatt catagcggtg
    18481 ccgccgggtt ggaccgtggc gcaggccatc gactacatgc gcgaaacgcc cgacctgccg
    18541 gcgcgctttt acgaaatcta tgccgttgac ggcgcgaaac gctggcaagg tgcaattccg
    18601 ctcgacgtgc tgctgcgctc gcgccggtcg gtaccgctcg ccgatctgat cgatgcggag
    18661 cgccaccgcg tgtccgtcac cgacgatctg gaagaagtcg cgcggctgtt cggcaagtat
    18721 aatctggtcg ccgcgcccgt agtcgacggc aacgatcgtc tggtcggcgt catcaccgtc
    18781 gacgacgtgg tcgacgtgat cgaggaggag gccgacgagg agatcaaggc gctcggcggc
    18841 gtcaccagcg acgaagaact ttccgacaat ttctggacca tcgccaaggg ccgcttcaac
    18901 tggctcctgg tcaatctcgc gaccgccttt ctggcttcat tcgtgctcgg cctgttcgag
    18961 gggcagctcg agaggatggt ggcgctggcg gtgctggccc cgatcgt
//
LOCUS       DATLJT010000665         5984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3487665,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000665 DATLJT010000000
VERSION     DATLJT010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487665"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VM910_15440"
     CDS             <1..493
                     /locus_tag="VM910_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI63956.1"
                     /translation="CRVPAENLIGEEGQGFKLIMHNFNSERMGMAASCTAYARVCLDE
                     AISYAKERKTFGKPIAQHQVIRHKLVDMAQRVAASQAMLEMLAWRLGQGESPVAEICM
                     MKNQATQTMAFCASEAVQIFGGAGFMRGIKVERIYREVKVNAIGGGTEEIMKDLASRQ
                     MGL"
     gene            609..1769
                     /locus_tag="VM910_15445"
     CDS             609..1769
                     /locus_tag="VM910_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI63957.1"
                     /translation="MLFTAEHEDVRRSLQRFIASEINPFVDEWEDAGAFPAHELFKKL
                     GDLGFLGLNKPAEFGGQGLDYSYALMMAEELGAIRCGGVPMAIGVQTDMATPALARFG
                     SDEVRREFLAPAIAGDAVACIGVSEPGAGSDVASIRTTARSDGDDYVINGGKMWITNG
                     AQADWICLLANTSDEQVHRNKSLICVSMNTKGVTVARKLDKLGMRSSDTAQIFFDNVR
                     VPRRNRIGDEGKGFAYQMIQFQEERLWGAAACLKAHEYIINETIEYTRNRKAFGQSIL
                     DNQIVHFKLAEMQTEVELLRSLIYRAAEALVAGEDVTKLATMAKLKAGRLGRELTDAC
                     LQFWGGMGFMNETPVSRAYRDSRLTSIGGGADEVMLMVLCKMMGTLPGMNQK"
     gene            1781..2437
                     /locus_tag="VM910_15450"
     CDS             1781..2437
                     /locus_tag="VM910_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI63958.1"
                     /translation="MVTLYHCHGARSFRPLWMLEEMGLAYELKMLPFPPRVFAKDYLA
                     LNPLGTIPLMIDGDTKMTESAGICHYLGVKYGPTPLMVETSDPHYGAFLNWMYFSDAT
                     LTFPQTLVLRYSQLEPEERRNPQVSTDYAKWFFGRLRAVEAATAKSETLCAGRFTAAD
                     IVIGYALRLAGTIGLAKDFGPNVAAYWARLQERDGYKRAIAAEQRAGIEQNVAPRIRA
                     "
     gene            2538..3359
                     /locus_tag="VM910_15455"
     CDS             2538..3359
                     /locus_tag="VM910_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI63959.1"
                     /translation="MDKKGSKSGHLMLITPERVFYAGLLGRPRERCPGAFHVYVAIRD
                     GLLLATAEGLTIHDELVVTMPNLRHTITSEYRAAICVAIEPESVGAGALEAWATRLSG
                     PDRASFARRFRATYEHLLNRQGRHDIDSAAFDTMCFGEPLPHRTLDPRVVKAIARIQT
                     FCGEPVTAASCAAQAGLSASRFLHLFKQETGISFRSFRAWKRARHLLHFANQDLNLAH
                     LAQDIGYPDSTHFSHSIRRFYGLKPRAIFSGSRDLAIYRSGAAEAADSEWTQEAG"
     gene            3417..4283
                     /locus_tag="VM910_15460"
     CDS             3417..4283
                     /locus_tag="VM910_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVI63960.1"
                     /translation="MSDKAVLTCALNGVLTDPKQHHVPVTPEEMAREAKVAFDAGASI
                     MHVHLRQQAPGKGHLPSWEVSVSREIQQAIREACPGVIINHTTGVPGPNNEGALACIR
                     ETRPEIAACNAGSLNYLKVKADNTWAWPPMMFDNSVEKVNDYLGVMKQVDTIPEFECF
                     DVGIVRCVGMYRQTGMYSGPLEYNFVMGVASGMPADPELLPILLKLKAPDAHWQVTAI
                     GRAEIWPLHQRCADLGGHLRSGLEDTFYLDDGTKVTSNGQLIEALAACARRAGREIAS
                     PAEARKIFGTKH"
     gene            4354..5970
                     /locus_tag="VM910_15465"
     CDS             4354..5970
                     /locus_tag="VM910_15465"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVI63961.1"
                     /translation="MAVIENTIATGGEAFKANRDGMLGQITRMRMLEERTRAASAAAK
                     DRFHQRGQLLPRERVALVLDPGSSFLELSTLAGYMFDIPDADKSVPGGGVIAGIGFVS
                     GVRCMVSANDAGIDAGALQPYGLDKTLRVQELALENKLPYVQLVESAGANLLRYRVED
                     FVRGGNIFRNLARLSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPP
                     LLKAATGEIATEEELGGAEMHTTISGLGDYLAEDDRDALRIAREIMAALKWDRPVASE
                     AAFKPPRYDAEELLGIMPMDHKRPVDMRQAIARFVDDSDFTEFSANYGPATVCGHARI
                     EGQAIGIITNNGPLDVPGANKATHFIQLCCQSRTPLLYMNNTTGYMVGRAYEEAGMIK
                     HGSKMIQAVTSATVPQVTLYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQA
                     AETMAIVTEAAAARRGKPVEKDRLDAMKAQIIGVFDGQMDVFSTSARVLDDGVIDPRD
                     TRSVLAEVLAICRESEVRQPQRMQFSVARP"
BASE COUNT         1161 a         1827 c         1923 g         1073 t
ORIGIN      
        1 ttgccgcgtg ccggccgaaa acctgatcgg cgaggaaggg caaggcttca agctgatcat
       61 gcataatttc aacagcgagc gtatgggcat ggcggcaagc tgcaccgcct atgcccgcgt
      121 ctgcctggac gaagcgattt catacgcgaa ggagcgcaag acattcggca agccgattgc
      181 gcagcatcag gtgatccgcc acaagctggt cgacatggcg cagcgcgtgg ccgcctccca
      241 ggcgatgctg gagatgctgg cgtggcggct cgggcaaggc gaaagcccgg tcgccgaaat
      301 ctgcatgatg aagaaccagg ccacgcagac catggccttc tgcgcctcgg aagcggtaca
      361 gatctttggc ggggcgggtt ttatgcgcgg cataaaagtc gagcgcattt atcgtgaggt
      421 gaaggtaaat gcgatcggcg gcggcacgga agagatcatg aaggatctcg ccagccgcca
      481 gatggggctg tgaaaccgcc ttccgggctt cgaagaggcg aatcggaacc tggagtttga
      541 atgaaacgga tcgagattct ggattcgcgc actcccgcga cagccccgga atgacgagga
      601 tgccaaaaat gctcttcacc gccgaacacg aagacgtccg ccgttccctg caaaggttca
      661 tcgcgagcga gatcaacccg ttcgtcgacg aatgggagga tgcaggtgca ttccccgcgc
      721 acgagctgtt caagaagctc ggcgatctcg gctttctcgg gctaaacaag ccggccgaat
      781 tcggcggcca gggtctcgat tacagctacg cactgatgat ggcggaggaa ctgggcgcca
      841 tcagatgcgg cggggtgccg atggccatcg gcgtgcagac cgatatggca acaccggcgc
      901 tggcgcgttt cggctccgac gaggtccgcc gcgaatttct cgcccctgcg atcgcgggcg
      961 atgccgtcgc ctgcattggc gtatccgagc caggcgccgg aagcgatgtg gcatcgatca
     1021 ggaccacggc gcgttcggac ggcgacgatt acgtcatcaa cggcggcaag atgtggatca
     1081 ccaacggcgc gcaggccgac tggatttgtc tgctcgccaa caccagcgac gaacaggtcc
     1141 atcgcaacaa atcgctgatc tgcgtatcga tgaatacgaa gggcgtcacg gtcgcacgaa
     1201 agctcgacaa gctcggcatg cgctcctccg ataccgcgca gattttcttc gacaatgtgc
     1261 gagtgcccag gcgcaaccgg atcggcgacg aaggcaaggg ctttgcctac cagatgatcc
     1321 agtttcagga ggaacggttg tggggcgcgg ccgcctgcct caaggcgcac gaatacatca
     1381 tcaatgagac catcgaatac acccgcaacc gaaaggcatt cggccaaagc atcctcgaca
     1441 accagatcgt gcacttcaag cttgcggaga tgcagaccga agtggagctg ttgcgctcgc
     1501 tgatctatcg cgcggccgaa gcgctggtcg ccggggaaga cgtgacgaag ttggccacca
     1561 tggccaagtt gaaagccggc cggttgggcc gggaactcac cgatgcctgc ttgcagttct
     1621 ggggcggcat gggctttatg aacgagacgc cggtgagccg cgcctatcgc gacagccgcc
     1681 tgacctcgat cggcggcggc gctgacgagg taatgctgat ggtattgtgc aagatgatgg
     1741 gcacgttgcc cgggatgaat caaaagtagg gatcgtttcg atggtcacgc tttatcattg
     1801 ccacggtgca cgctcgttcc ggccgctgtg gatgctggaa gaaatggggc ttgcctacga
     1861 gctgaagatg ctgccgttcc cgccgcgggt atttgccaag gactatctcg cgctcaatcc
     1921 gctcggcacc attccgctga tgatcgatgg cgacacgaag atgacggagt ccgccggcat
     1981 ctgccactat ctcggtgtca aatatggtcc gacgccactg atggtcgaga cgagcgatcc
     2041 acactacggc gcgttcctga actggatgta tttttccgac gcgacactga cctttccgca
     2101 gacgctggtg ctccgctaca gccagctcga gccggaagag cggcgcaatc cgcaggtctc
     2161 gacagattac gccaaatggt ttttcggacg cttgcgtgcg gtcgaggcgg cgaccgccaa
     2221 gtcagagaca ttgtgcgcgg ggcgctttac cgcggccgat attgtgatcg gctatgcgct
     2281 gcggcttgcc ggcacgatcg gactggcaaa ggatttcgga cccaatgtcg cggcttattg
     2341 ggcgcggctg caagaacgtg acggctacaa gcgggcgatt gcggccgagc agcgggccgg
     2401 gatagagcag aacgtggcgc cacgcatcag ggcgtgacct tgcgccacgc cgaattcatg
     2461 gccccgcagg agcgcatttt gggctatggt tcgacccacc gcagcggtcc caaagagtcc
     2521 gcgcgaggag gacagccatg gacaagaagg gaagcaaatc cggccatttg atgctgatca
     2581 cgcccgagcg ggtattttac gccggcctcc tcggccgccc cagggaacgt tgcccgggcg
     2641 cgttccatgt ctatgtcgcg atcagggatg gactgctgct cgcgacagcc gagggcctca
     2701 cgatccatga cgaactggtc gtgaccatgc cgaatctccg ccacaccatc accagcgagt
     2761 atcgcgcggc gatttgtgtg gcgatcgagc cggaaagcgt cggcgccggc gcgcttgagg
     2821 cctgggcgac gcggctgtca ggcccggatc gggcgtcgtt cgcgcgccgc tttcgcgcga
     2881 cgtacgagca cctgctcaac cggcaaggcc gccacgacat cgacagtgcc gcattcgata
     2941 cgatgtgctt cggcgagccg ctgccgcatc ggaccctcga tccgcgcgtg gtcaaggcga
     3001 ttgcccggat ccagaccttt tgcggagagc ccgtgacggc cgcaagttgc gcggcgcagg
     3061 ccgggctttc cgcctcgcgc ttcctgcatc tgttcaagca ggagaccgga atctcgttcc
     3121 gctcatttcg ggcctggaag cgcgcgcggc atctgctgca ttttgccaac caggacctca
     3181 acctcgcgca tctggcgcag gacatcggct atcccgactc gacgcatttt tcgcactcga
     3241 tccgcaggtt ttacggcctg aaaccgcgtg cgatcttctc cggttcacgc gatctcgcaa
     3301 tttaccgcag tggcgctgcg gaggccgcgg atagcgagtg gacgcaagaa gccggctgag
     3361 gtgggtgcac atgatcgcgg gacatcgctg caatcccgct aatcgaaggc cattccatga
     3421 gcgacaaggc tgtcctcacc tgcgcgctga acggtgtgct gaccgatccc aagcagcatc
     3481 atgttcctgt cacgcccgag gagatggcgc gcgaggcgaa agtcgcattt gatgccggcg
     3541 ccagcatcat gcacgtccat ctgcgccagc aggcgcccgg caagggccat ctgccctcct
     3601 gggaggtcag tgtcagcaga gagatccagc aggcgatccg cgaagcctgt ccgggcgtga
     3661 tcatcaacca taccaccggc gttcccggcc ccaataatga gggtgcgctg gcctgcatcc
     3721 gggagaccag gcccgagatc gccgcctgca acgcgggttc gctgaattat ctgaaggtga
     3781 aggccgacaa cacctgggcc tggccgccga tgatgttcga caattcggtg gagaaggtga
     3841 acgactatct cggtgtcatg aagcaggtcg acacgatccc ggagttcgaa tgtttcgacg
     3901 ttggaatcgt gcgctgcgtc ggcatgtacc ggcagaccgg catgtattcc gggccgctcg
     3961 aatataattt cgtgatgggc gtcgcttcgg gcatgccggc cgacccggaa ttgttgccga
     4021 tcctgctcaa gctcaaggcg ccggatgcgc attggcaggt gaccgcgatc ggacgcgccg
     4081 aaatctggcc gctgcaccag cgctgcgccg atctcggcgg ccatctgcgc tccggactgg
     4141 aagatacgtt ctatctcgac gacggcacca aggtcacatc gaacgggcag ttgatcgagg
     4201 cgctggctgc ctgcgcgcgc cgcgccgggc gggagatcgc ctcgccagcc gaggcgcgaa
     4261 agatcttcgg gacgaaacat tgatccaacg tcgtcattct gggttcgcga cttcgtcgtt
     4321 tccggattga caaaaggaga gaccacgccg ctcatggccg tcattgaaaa caccattgcg
     4381 accggcggcg aggccttcaa ggccaatcgc gacgggatgc tcgggcagat cacgcgcatg
     4441 cgcatgcttg aggaacgcac gcgcgccgcg tctgccgccg caaaggaccg ctttcaccag
     4501 cgcgggcagc ttttgccgcg cgagcgcgtg gcgctggtgc tcgatcctgg cagttccttc
     4561 ctcgaactct cgacgctcgc cggctacatg ttcgacatcc ccgatgccga caagagcgta
     4621 ccaggcggcg gcgtgatcgc ggggatcggc tttgtgtccg gcgttcgctg catggtcagc
     4681 gccaacgatg ccggcatcga tgccggagcg ctgcagcctt atggcctcga caagacgctg
     4741 cgggtgcagg aactggcgct ggagaacaag cttccttacg tacagttggt cgaaagcgcc
     4801 ggcgcgaatc tgttgcgcta tcgcgtggag gattttgtcc gcggcggcaa catctttcgc
     4861 aatctggcgc ggttgtcggc cgcgggtctt ccggtcgtca ccgtgaccca tggctcctcg
     4921 actgcgggcg gggcttatca gaccggcttg tccgactaca tcgtgatggt gcgcggccgc
     4981 acgcgcgcgt tcctcgccgg tccgccgttg ctgaaagctg caaccggcga gatcgcgacc
     5041 gaagaagagc ttggtggcgc cgagatgcac accacgatct cgggactcgg cgactatctc
     5101 gccgaggatg accgcgatgc gctccggatc gcgcgcgaga tcatggccgc gctgaaatgg
     5161 gatcggccgg tcgcgagcga agccgcgttc aagccaccgc gttacgatgc ggaagaactg
     5221 ctcggaatca tgccgatgga tcacaagcgc ccggtggaca tgcgccaggc gatcgcgcgc
     5281 tttgtcgacg attccgattt cacggaattt tcggccaatt acggcccggc caccgtctgc
     5341 ggccatgccc gtatcgaggg gcaggcgatc ggcatcatca ccaacaacgg gccactcgac
     5401 gtgcccggcg ccaacaaggc cacgcatttc atccagctct gctgccagag ccgcacgccg
     5461 ctcctctata tgaacaacac caccggctac atggtcggcc gtgcctatga ggaggcgggc
     5521 atgatcaagc acggttcgaa gatgattcag gccgtcacgt cggcgaccgt gccgcaggtc
     5581 acgctctatt gcggtgcttc cttcggcgcc ggcaattatg gcatgtgcgg tcgcggcttt
     5641 catccgcgct tctgcttctc ctggcccaac gccaagaccg ccgtgatggg cggcgagcag
     5701 gcggccgaga cgatggccat cgtcacggag gctgcggccg cgcgtcgcgg caagccggtc
     5761 gagaaagaca ggctggatgc catgaaggcg cagatcatcg gcgtattcga cggacagatg
     5821 gatgtgtttt cgaccagcgc tcgcgtgctc gacgacggcg tgatcgaccc gcgcgacacg
     5881 cgaagcgttc tcgccgaggt gctcgcgatc tgccgcgaat ccgaggtgcg gcagccgcaa
     5941 cgcatgcagt tctcggtcgc gcggccatga ccggcaaaat gaag
//
LOCUS       DATLJT010000666         1979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2338030,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000666 DATLJT010000000
VERSION     DATLJT010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338030"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VM910_15470"
     CDS             <1..243
                     /locus_tag="VM910_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI63962.1"
                     /translation="AVTDKTIVIPGHGQPVSNKAELTEYRDMLVAIRENVAKLKQQGR
                     SLEETIAAKPTAAFDAKWGQFVITPVLFTRLVYQGV"
     gene            331..768
                     /locus_tag="VM910_15475"
     CDS             331..768
                     /locus_tag="VM910_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HVI63963.1"
                     /translation="MTVSKPAPHIDIPVKLKEVKTVFSIDALAFEGDLPASIFHLQLI
                     ENDIADWKAKSEVIAVFHTNAGHVTLNDTAYNADRNITTGNPYKGLIADLMKRGVQVE
                     LCGATAKAHGWVNRDLLPGIKVNTDAMARTTQLVQEGFVKIME"
     gene            1085..1339
                     /locus_tag="VM910_15480"
     CDS             1085..1339
                     /locus_tag="VM910_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63964.1"
                     /translation="MTEKLQLRDAVYRLEDTIREIRDRFADNQGRVDEIAFEESKADP
                     LFALAVPLVEQIAERAPLPGVVQLATEIINRCVAELVGSE"
     gene            1503..>1979
                     /locus_tag="VM910_15485"
     CDS             1503..>1979
                     /locus_tag="VM910_15485"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI63965.1"
                     /translation="MRRRQFITLLAGAGLLAVAPRAFATEYPTRPVRWIIGFPPGGGA
                     DQVARLIAPSLSERLGQQVVIENRPGAGTFLSVQAVLNSAPDGYTLLHYGGSTLMNAK
                     ISANPPPLEDGIAPVAGLVAYPMILVAHPLFPAKTVPELIDYAKSNPGKVTMASFGI"
BASE COUNT          427 a          594 c          586 g          372 t
ORIGIN      
        1 gccgtaacgg acaagacgat cgtcatcccc ggccatggcc agccggtgag caacaaggca
       61 gagctgactg aatatcgcga catgctcgtt gcgatccgtg aaaacgtcgc aaagctcaag
      121 cagcaggggc gatcgctcga agagacgatt gccgccaagc cgaccgccgc gttcgacgcc
      181 aaatggggtc aattcgtcat aacgccggtg ctcttcacgc gcctggtcta ccagggcgtc
      241 tgacaccgct gcgctgagtt gttcagctcc cgcgaccgag ttgtgccatg taacgcgcaa
      301 gaatagcgtc gctccgaaag gaaccaagac atgaccgttt caaaaccagc gccgcacatc
      361 gatattccgg tgaagctcaa ggaagtaaaa acggtgttca gtatcgacgc cttggcattc
      421 gaaggggatc tgcccgcttc gatcttccac cttcagctca tcgagaatga tatcgccgat
      481 tggaaagcca agtccgaggt catcgcggtg tttcacacca acgccggcca cgtgacgttg
      541 aacgataccg cctacaatgc tgaccggaac ataacaaccg gcaatcccta caagggtctc
      601 atcgccgacc tgatgaaacg cggcgtgcaa gtcgagttgt gtggtgcgac cgcgaaggcg
      661 cacggatggg tcaacaggga cctgcttccc ggcatcaagg tcaacacaga cgccatggca
      721 aggacgacac agctcgttca agaaggattc gtgaaaataa tggaataggg tcagggggcg
      781 cgccgtggag tgaccgacga gcgtgggcac gccgccgggt ctgctgggcc gcggaagtcg
      841 cgtagggtgg gcaaaggcgc gctgctcgat tgctcgcgtg gcgacgatgg ccacgctgct
      901 atgcttttta ttgaggctag attcatcgca ctcgggcgca gccccctcta tttcggcctt
      961 gtaccacctt gatgagttgc gcatttcatt cggtgcagtg aagctactcc ccataataac
     1021 gttgccgggt tatcgttacc gcccgtttca acgcatcgga ccgacatgag ctcggagagt
     1081 gacaatgact gagaaattgc agctgcgaga tgcggtctac aggctcgaag acactatccg
     1141 cgagatccgc gaccgattcg cggacaatca gggaagggtc gacgagattg ccttcgagga
     1201 aagcaaggca gacccactgt tcgcattggc agtcccgttg gtcgaacaga tcgccgagag
     1261 agcaccgctg cccggggtgg ttcagctggc cacagagatc ataaatcggt gcgttgctga
     1321 gcttgtcggg agcgagtgag caagtcctac tactccacga cccgccgatt ttacaaagcg
     1381 cgcgcaggtc aggcgaggca agtaacgcag gggtagtggg ctcctttcaa ccttaaagcc
     1441 gcgaaagcat tgggcgtgag aattccggac tcattacttg ccactgccga cgaggtcatc
     1501 gagtgaggcg ccgccagttc atcacgctgc tcgccggtgc cggattgttg gctgttgcgc
     1561 cgcgcgcatt cgccacagag tatccaaccc gcccagtgcg gtggatcatc ggttttccgc
     1621 ctggcggtgg tgccgaccag gtggcgcgcc ttatcgcgcc gtcgctctcg gaacggctgg
     1681 gccagcaggt cgtaatcgaa aaccggccgg gtgccggcac cttcctctcc gtgcaagcgg
     1741 tgctcaactc agcgccagac gggtacacgc tgctccacta cggcggctcc acgctgatga
     1801 acgcgaaaat ctcggcgaac ccgcccccgc tggaggacgg catcgcgccg gtcgccggcc
     1861 tcgtcgccta tcccatgatc ctggtcgcac atccattgtt ccccgccaaa acggttcccg
     1921 agctgattga ctacgccaag tccaatcccg gcaaggtcac catggcttcg ttcggcatc
//
LOCUS       DATLJT010000667         3894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4406114,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000667 DATLJT010000000
VERSION     DATLJT010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406114"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..1983)
                     /locus_tag="VM910_15490"
     CDS             complement(394..1983)
                     /locus_tag="VM910_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI63966.1"
                     /translation="MSIATLRDENERLKALLAETQAALSEHQAALATSEEARRRLEVI
                     LGELRREKFGAKSEKLRPDQYHLPLEDVEIAQGVLDSAQEKATAIIRGRSRAGSDQDR
                     DRNRSCLPAHLPRVERIIEPASTLCPCGCGAMTKIGEDVSKRLDVIPAQWRVLVTRRP
                     KYVCRRCSGAVVQAHAPEHVVPGGLPTEAAIAHVIVSKFGDHTPFYRQAGIYARQGIR
                     LDRATLGNWSGRACFHLQPVGDHMRRHLATADRLFMDETTAPVLDPGRGETKKGYFWA
                     IASDDRGHSGPSPPIVLFRYASGRSGTFAEQFLAGFNGRFLQCDGYDGYDRLSELARS
                     QGPWTLVHCWSHLRRRFVKLARNSKSPIAEAAVRQIAQLYAIEATVRGLSPDIRLAAR
                     KEYSLPIVTALKPWFEKQLSMISSGSTLAEDIRYALNHWPGLTRFLDDGRLELDTNPV
                     ENAIRPICLTRKNALFAGHEVGAENWALLASIVATCKLNDVNPVTYIAQTLEAIIAGH
                     PQSRIEDLMPWRFRKMSSQHQ"
     gene            complement(2052..2405)
                     /gene="tnpB"
                     /locus_tag="VM910_15495"
     CDS             complement(2052..2405)
                     /gene="tnpB"
                     /locus_tag="VM910_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273520.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HVI63967.1"
                     /translation="MIAPGAELKIYIATRPVDFRCGHDGLASKVQEMLGLDPFSGAAF
                     VFRSKRADRIKILVWDRTGLVLVHKRLEGCKFVWPRIADGVMRISPAMFAALFEGLDW
                     RLVRPEEARRPQAAG"
     gene            complement(2402..2824)
                     /locus_tag="VM910_15500"
     CDS             complement(2402..2824)
                     /locus_tag="VM910_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI63968.1"
                     /translation="MGLDGKKDVHLDGSSVGVVSRLEVLEGPSGRRVRSEAERARIAA
                     ESLLPGAQVSEVARKHGATRWQVYDWRRRFRQRGELRSCEASQPPFAPLVVEGPLEER
                     HIPTVKLEIAIGDVVVRTDAAIDGEQLSRVIRAVRASR"
     gene            3683..3804
                     /locus_tag="VM910_15505"
                     /pseudo
     CDS             3683..3804
                     /locus_tag="VM910_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340144.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          768 a         1252 c         1125 g          749 t
ORIGIN      
        1 agccgacaga ttctttcaca ttcgccaatc aatcaaccgt gacggtgcgg atggtcggcg
       61 cgctgggcac cgggccggtt aaatggccag cggcccatgc cgagcccgcc caggcaaatt
      121 gggcgtcagg ttagccgttt ggttagctgg ctggttgggt tggttgggtt gggttggtta
      181 ggaggtggcc ctttcgagcc gcctcttcgg ttgcctactg acagacgtaa ttgatgccgt
      241 ccccaccttc gacaatacaa acttccgtcg ccttcggcgc ttcttcaccc gaacctaaga
      301 gaaagccgtc gatcgaagcc tgcaattgaa gcgtggtacc cttgtttccg gttgagcggt
      361 gccgctccca ttcgtaagcg cttagccgca accctattga tgctggcttg acattttgcg
      421 gaatcgccat ggcatgaggt cttcgattct gctttgggga tggccggcga tgatcgcctc
      481 gagcgtttgg gcgatgtagg tgacggggtt cacgtcgttg agcttgcagg tggcaacgat
      541 cgacgcaagc aaggcccaat tttcggcccc gacctcatga ccggcgaaga gcgcattttt
      601 tctggtcaag cagatcggcc ggatcgcatt ttcgaccgga ttggtgtcga gctcgagacg
      661 cccgtcatcg aggaagcggg tcagtcccgg ccaatgattg agcgcgtagc ggatgtcctc
      721 ggcgagcgtc gagccgctcg atatcataga cagctgtttc tcgaaccacg gctttaacgc
      781 ggtgacgata ggcagcgaat actccttgcg cgcggccaaa cggatgtccg gcgataggcc
      841 gcggaccgtg gcttcgatgg cgtaaagctg tgcgatctgc cggactgcag cctcggcgat
      901 cggcgacttg ctgttgcgcg ccaatttgac gaagcgccgg cgcagatggc tccagcaatg
      961 cacgagcgtc catggccctt gtgatcgagc cagctccgag agccggtcat aaccgtcata
     1021 tccgtcacat tgcaggaagc gcccgttaaa accagccagg aattgttcgg caaaagtgcc
     1081 gctgcgcccg gatgcatagc ggaacagcac gatcggcgga cttgggccgc tatggccgcg
     1141 atcgtcggag gcgatcgccc agaagtagcc tttcttcgtc tcgccgcgcc cgggatcgag
     1201 caccggcgcc gtggtttcgt ccatgaacag acgatccgcc gtggccagat ggcggcgcat
     1261 atgatcgccg acgggctgga gatggaagca ggcgcggcct gaccagttgc ccagcgtcgc
     1321 ccgatcgagc cggatccctt gtcgcgcata gatcccagcc tgacggtaaa acggcgtatg
     1381 gtcgccgaac ttggagacga tcacgtgcgc aatggccgcc tcggtcggta gcccgccggg
     1441 cacgacatgc tccggcgcgt gtgcctgcac gacagcgccc gagcagcgac ggcagacgta
     1501 tttcgggcgg cgcgtgacca aaacccgcca ttgcgccggg atcacgtcga ggcgtttgct
     1561 gacgtcctcg ccgatcttcg tcatggcacc gcaaccgcac ggacagagcg tgctcgcagg
     1621 ctcaatgatc cgctccaccc gcggcaaatg agcaggcaag caactccgat tgcgatcacg
     1681 gtcttgatcc gatccggcgc gcgatcgccc cctgatgatc gccgtcgcct tctcctgcgc
     1741 cgagtccagg acgccttgcg cgatctccac gtcttcgagc ggcaaatgat attgatctgg
     1801 cctcagcttc tcggacttcg caccgaactt ctcccggcgt agttcaccga gaatgacctc
     1861 caaccgacgc cgcgcttctt ccgacgtcgc cagcgcggct tgatgctcgc tcaacgccgc
     1921 ttgcgtttct gccaaaagcg ccttcaagcg ttcgttctcg tcgcgcagcg tcgcgatgct
     1981 catgcgctat gtcgaacaca tcttcgccac cgccgccatg cgtaactcgg ctccgagtca
     2041 ttctgccgca gttatccagc ggcctgtgga cgccgcgctt cctccgggcg gaccaacctc
     2101 caatccaggc cctcgaacaa cgccgcgaac atcgccggcg atatccgcat cacgccgtcc
     2161 gcaatccttg gccatacgaa cttgcagccc tcgaggcgtt tgtgaactaa caccaggccc
     2221 gttcgatccc aaaccagaat cttgatccgg tccgcccgtt tcgaccggaa cacgaaggcc
     2281 gcaccgctga acgggtcgag accgagcatc tcctgcacct tcgaagcaag accatcgtgg
     2341 ccacagcgga agtcgacagg ccgcgttgcg atgtagatct tcaactcggc gccgggggca
     2401 atcatcgtga cgcccgcacc gcgcgaatca cccgagacag ctgctcccca tctatcgccg
     2461 cgtccgtccg caccacgacg tcgcctatcg caatctcgag cttaaccgtc ggaatgtggc
     2521 gctcctctaa cggcccttcc acaaccagcg gcgcgaacgg cggctgcgac gcttcgcacg
     2581 accgcaattc gcctcgctgt cgaaagcgtc gtcgccaatc gtaaacctgc cagcgcgttg
     2641 ccccgtgctt gcgcgccacc tccgacacct gagcgccggg cagcagactc tcggcggcga
     2701 tccgcgccct ttcagcctcc gaacgcacac gacgcccgga cggtccttca agcacttcaa
     2761 gccggctgac caccccaacc gacgagccgt ccaaatggac gtcctttttg ccgtccaatc
     2821 ccatccaaaa cctccgtcca agccggaggc cttcttcgca catttaatct acccctgaag
     2881 caagggtacc gacttcgcgc ttacggctgc ttggctggac gatactttga aacaacaacg
     2941 gtaaggccgc ctcagttggc agcatctttc aatagaagta ataattcccc gccgcgcgtg
     3001 tcaacaaaac agcccccggc ggggaggctg attgacgtct atgctaaaga caatccggtt
     3061 aagcgagacg agatcgcggc gcaccaagtc ctcgtccagc gatctgatca agcaattcgt
     3121 tcagtgcaat gaacgatcgc agttcgtgcg gcgtaatgaa cgatggagcc cgaatgttcc
     3181 agtgtgcttg ctttaccggg catcggccag ccgtgcattg agcagatgta tgaccgctcg
     3241 tgtattttcc ttcaagtccg ctacgaccga ttcggacatg acttacctcc tattgcgagg
     3301 agcggccgcg tcgaccagct gtgtgcacgt aaagcggcgt cacagccgat tgccttggtt
     3361 acgccggaca attttaaaca caaggagtaa gacaccgccg agtaaggaaa gaaagccgac
     3421 cgcgattact ctgcccaatg ggcgaagatg gaaccgcttt gtcttggcaa actgagcgat
     3481 cccggacgca gcggcaagaa caaccgcctg ttagtcgagg cagtattatg gatcgcccac
     3541 acgggcagtc catggcgcga tctgccgtgc gccttcggcc actggaacag cgtgtacacg
     3601 cgtttccgcg actgagcgga ggcggacgtg tggaagcgcg cctgttcgac gcggtctcgg
     3661 acagccggac atggaatacg ccatggtcga cgccaccatc gtcaaggtcc atcgccacgg
     3721 acagggcgca aaagggggac tcaaaggcag gtcatcggcc gttcgaaagg cggcatgacc
     3781 accaaaatcc tggcgctcac cgatgcgctc agaaatctcg tgcgtttcgt gctgccgccc
     3841 ggacggcgct tcgacacagt cggcgttccg ccgctcatcg aagggttggc gttc
//
LOCUS       DATLJT010000668         5723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2338068,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000668 DATLJT010000000
VERSION     DATLJT010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338068"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VM910_15510"
     CDS             complement(<1..1098)
                     /locus_tag="VM910_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI63969.1"
                     /translation="MRRRHFLGVSLAIVVAGMANSAIAQSEIKIGEINSYSLLPAFTD
                     PYRKGWQLAVEEVNAAGGINGKKLVVISKDDGGKPADAQTAANELVSSENVAMLVGTF
                     LSNIGLAVSDFANQKKVFFLAAEPLTDALTWSKGNRYTFRLRPSNYMQAAMLAEAAAE
                     LPAKRWATIAPNYEYGQSAVGVFKKLMSEKRPDIQWVDEQWPPFNKIDAGPVVQALAA
                     ANPEAILNVTFGADLVKFVREGSTRGLFKDKTVISFLTGEPEYLDPLKDETPEGWIVT
                     GYPWYAINTPEHQAFLKAYQAKYNDYPRLGSIVGYETIKAAAAILTKANSTDPEKLIA
                     ATEGLSVGSPFGEITFRKIDHQSTLGAFVGKT"
     gene            complement(1123..1707)
                     /locus_tag="VM910_15515"
     CDS             complement(1123..1707)
                     /locus_tag="VM910_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HVI63970.1"
                     /translation="MSAIIRKIVTVVEETHIEMGQRVSPPTRRAAAIAVIENPFAGRY
                     VEDLSPLIAIGEELGELLAKKAVAALGIDGAKAQSYGKAAAVGENGELEHAAAILHPK
                     MGAPVRKVLSKGAALIPSSKKRSGPGTTLDIPLGHKDAAFVRSHFDGMEVQINDAPRA
                     NEIMVAVAVTDSGRPLPRVGGLTVAEVKGEDGLR"
     gene            complement(1726..2619)
                     /locus_tag="VM910_15520"
     CDS             complement(1726..2619)
                     /locus_tag="VM910_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HVI63971.1"
                     /translation="MRRLPQIALLQDGKRLHLQDGPIDLIVEAKGEDAAVRAAYDAAA
                     RRFDGLLDELCAELVELRKAAHPKHCALEGIVARRMHAAVAPFAADRFITPMAAVAGS
                     VAEEILGAMQSVAPLKRAYVNNGGDIALHLEDDEQFCVGLMDRPDRQGVMRTMIVDAA
                     DPSRGVATSGRHGRSFSLGIADAVTVLARTASQADAAATIIANAVDLPGHPAVLRCPA
                     NELQPDTDLGPRLVTRDVGELSEYEIHAALAAGETVARQLLAAGRIDGAVLFLLGQTA
                     VVGTRGLDARQPEARAALQFA"
     gene            complement(2616..4067)
                     /locus_tag="VM910_15525"
     CDS             complement(2616..4067)
                     /locus_tag="VM910_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HVI63972.1"
                     /translation="MVTDATSASSGDKIRCDACPVMCYIKPGAAGACDRYANHDGKLV
                     RVDPHIVLERTMSHGGRVVPFQATGDWDGKIVHQSDIVVTAIGAGTTYPDYKPAPFIV
                     SSEIDGIDMVTVVTEGIFSYCGVKVKIDTDRYLGPETATVRAQGEAVGHVTTSEYGSQ
                     MLSLGGVHHLTGGSKKEGRVTCDTLMDLSNCKPVELTIDGGAKVVVQAGRPPIVNGVT
                     EERMRVGCGSATIGMFAKQWHGKVDEVVVVDDHITGVLSEHQAGKLLDIADTGVKMKG
                     RRSTPGRYFQVADPGTGWGGTNISDPLSILGPFDAREARPGLSMLMVSTTGEHAGYYV
                     LDDALRPVAQDMPLDLKLSVERIQENCEPALCTVLFMGGAGGSLRAGVTDNPVRLTRS
                     VKDALTRVTSGGAPVYVWPGGGITFMVDVTQMPAGAFGYVPTPALVAPIEFTMRLSDY
                     AALGGHMDYVRPLVSLKDSAEIRPMPYIPGRRA"
     gene            4348..4830
                     /locus_tag="VM910_15530"
     CDS             4348..4830
                     /locus_tag="VM910_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI63973.1"
                     /translation="MTQSMIRLTVNGRIHDVVAAPDTALLYVLRNDLALNGPKYGCGL
                     GECGTCTVLIDGVAARSCVIPISGCIDRDIMTLEGLGTRERPDPVQDAFIKEQAAQCG
                     YCLNGMIMTTKALLKTNPHPTEAEIVEALRYNLCRCGAHVEIIRAAMRASGRLAEARD
                     "
     gene            4830..>5723
                     /locus_tag="VM910_15535"
     CDS             4830..>5723
                     /locus_tag="VM910_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI63974.1"
                     /translation="MVQVPQGSLSVVQSAGTATSFEAFIKITAEGSVTAYNGHVDLGT
                     GIRTALGQIVAEELDVSFARVVVVLGDTAQVPDQGATIASETIQITAVPLRKAAAQAR
                     QFLIARAAERLEVGVDDLSIEDGLVRGPNNRSVTYGELIGDDTIRLELADDVPVKAVD
                     GYSIVGKSVPRVDLPAKATGELVYVHDVRVPGMLHGRVVRPPYAGVDAGPFVGTSLIA
                     VDEASVRHIPGMVAVVRIGDFVGVVAEREENAVKAAAQLKLSWKPTPTLPDLKDIETA
                     LRANPSTPRTLIDKGNVDAAIG"
BASE COUNT         1018 a         1873 c         1787 g         1045 t
ORIGIN      
        1 cgtcttgccg acgaaggcgc cgagcgtcga ctggtggtcg atcttgcgga aggtgatctc
       61 gccgaatggc gagccgaccg acaagccttc ggtcgccgcg atcagctttt cgggatcggt
      121 ggaattggcc ttggtcagga tcgcggcggc cgccttgatg gtctcgtagc cgacgatcga
      181 gccgagccgc ggatagtcgt tgtacttcgc ctgataggcc ttcagaaacg cctggtgctc
      241 gggcgtgttg atcgcatacc agggatagcc ggtgacgatc cacccttcgg gggtttcgtc
      301 cttcagagga tcgagatatt ccggttcgcc agtcaggaac gagatcaccg tcttgtcctt
      361 gaacaggccg cgggtcgaac cttcgcgcac gaacttcacc agatcggcgc cgaaggtgac
      421 gttgaggatg gcttccggat tggcggcggc gagagcctgc accaccggcc ccgcgtcgat
      481 cttgttgaag ggcggccact gctcgtcgac ccactggatg tcgggccgct tctccgacat
      541 cagcttcttg aacacgccca ccgcggattg gccgtattca taattcggcg cgatcgttgc
      601 ccagcgcttg gcgggcagtt ctgccgcggc ttcggccagc atcgcggcct gcatgtagtt
      661 ggagggacgc aggcggaagg tgtaacgatt gcccttcgac caggtgaggg catcggtcag
      721 gggttcggcc gccaggaaaa agaccttctt ctggttggcg aagtcgctca ccgcaagccc
      781 gatgttggac aggaacgtgc ccacgagcat cgcgacgttc tcgctcgaga ccaattcgtt
      841 ggcggcggtc tgcgcgtccg caggcttgcc gccgtcgtcc ttggagatca cgaccagctt
      901 cttgccgttg atgccgccgg ccgcattgac ctcctcgacg gcgagttgcc agcccttgcg
      961 atagggatcg gtgaaggccg gcaaaagcga gtagctgttg atctcgccga tcttgatctc
     1021 gctctgggcg atggccgagt tcgccatgcc ggcgaccacg atcgccaggc tcactcccag
     1081 aaaatgtctc cgtcgcatcc cttgcctcca ctgttgaaga aactagcgca aaccgtcttc
     1141 gcccttgact tcggcaacgg tcagaccgcc gacccgcggc agcggccgcc cactatcggt
     1201 gacggcgacc gccaccatga tctcgttggc gcgcggcgca tcattgatct gcacttccat
     1261 gccgtcaaaa tggctccgca cgaaggcggc gtccttgtgg ccgagcggaa tgtcgagcgt
     1321 cgtgccggga ccgctgcgct tcttcgacga cgggatcagg gcagcacctt tgctcagcac
     1381 cttgcggaca ggcgcgccca tcttcggatg caagatcgcc gccgcgtgct cgagctcgcc
     1441 attttccccg acggccgcag ccttgccgta gctttgcgcc ttcgcaccat cgatgccgag
     1501 tgctgcgacc gccttcttcg cgagcaattc gccgagttcc tcgccgatgg cgatcagcgg
     1561 cgagagatcc tcgacataac gcccggcaaa ggggttttcg atgaccgcga tcgcggcggc
     1621 acgtcgtgtc ggcggcgaaa ccctttgtcc catctcgata tgggtttcct cgacgaccgt
     1681 gacgatcttg cgaatgatgg cactcatcgg tttccgccct gttgcttacg caaattgcag
     1741 cgcagcgcgc gcttctggct gacgcgcatc gagcccgcgc gttccgacga cggccgtttg
     1801 gccaaggaga aacagcacgg cgccgtcgat ccgtcctgcc gcaaggagct gtcgcgcgac
     1861 agtttcgccc gcagcgagcg ctgcgtgaat ctcatattcc gagagctcgc cgacgtcgcg
     1921 ggtgaccagg cgcgggccga gatccgtgtc gggctgcaac tcattcgccg gacaacggag
     1981 aacggcggga tgtccaggca ggtcgaccgc gttcgcgatg atcgtggccg cggcgtccgc
     2041 ttgcgacgcg gtccttgcca gaaccgtgac ggcgtcggcg atcccgagcg aaaagcttcg
     2101 gccatgacgc ccgctggtcg ccacaccgcg cgagggatcg gcagcatcga cgatcatggt
     2161 ccgcatcacg ccctgacggt cgggccgatc catcaggccg acacaaaact gctcgtcatc
     2221 ttcgaggtgc agcgcgatat cgccgccatt gttgacatag gcgcgtttca gcggcgccac
     2281 gctctgcatg gcgcccagaa tctcttccgc cacgctgccg gcgaccgccg ccatcggcgt
     2341 gataaagcga tcggcggcaa acggcgcgac agcagcatgc atacggcgcg cgacgatgcc
     2401 ctcgagcgcg caatgcttcg ggtgtgccgc cttgcgcagc tcaaccagtt ccgcgcagag
     2461 ctcgtcgagc aggccgtcaa atcgccgcgc cgccgcgtcg taggccgcac gaacggcggc
     2521 gtcctcgccc tttgcctcga cgatcaggtc gatcggacca tcctgcaaat gcagtcgctt
     2581 gccgtcctgg agaagggcga tttgcggcag tcgcctcatg cgcgtcgtcc cggaatgtac
     2641 ggcatcggac ggatttctgc gctgtccttc aaagaaacca gcgggcgcac ataatccata
     2701 tggccgccca gggccgcata atccgacagc cgcatcgtga actcgatcgg cgcgacgagc
     2761 gcaggcgtcg gcacgtagcc gaaggcgccg gccggcatct gcgtgacgtc gaccatgaag
     2821 gtaataccgc cgcccggcca gacatagacc ggcgccccgc cgctggtgac gcgcgtcagc
     2881 gcgtccttga ccgatcgcgt cagccgtacc ggattgtcgg tgacgccggc gcgcagcgat
     2941 ccgccggccc cgcccatgaa cagaaccgtg cacagcgccg gctcgcaatt ttcctggatg
     3001 cgctcgaccg acagtttcag gtcgagcggc atgtcctgcg cgaccggccg cagcgcgtca
     3061 tccagcacgt aatagccggc gtgctcgccg gtcgtcgata ccatcagcat gctcaggccc
     3121 ggtcgcgcct ccctggcatc gaacggaccg aggatcgaca acggatccga gatattggtg
     3181 ccaccccatc ccgttccggg atcggcaacc tgaaaataac ggccgggcgt cgaccggcgg
     3241 cccttcatct tgacgccggt gtcggcaatg tcgagcagct tgccggcctg atgctcggag
     3301 agtacgccgg tgatgtggtc gtcgacgacg acgacctcgt cgaccttgcc atgccactgc
     3361 ttggcgaaca tgccgatggt cgccgaaccg cagccgaccc gcatccgctc ttcggtgacg
     3421 ccgttgacga tcggcggccg gcccgcctgc accaccacct tcgcgccgcc gtcgatcgtg
     3481 agctcgaccg gtttgcaatt gctcaggtcc atcagtgtgt cgcaggtgac gcggccctcc
     3541 ttcttcgagc cgccggtcag gtgatgcacg ccaccgagcg acagcatctg cgaaccatat
     3601 tcgctggtcg tgacatggcc gaccgcttcg ccttgcgcgc gcaccgtcgc ggtctcgggg
     3661 ccaagatagc gatcggtatc gatcttcacc ttgacgccgc agtaggagaa gatgccttcg
     3721 gtcacgaccg tgaccatgtc gatgccatcg atctcggagg agacgatgaa cggcgcgggc
     3781 ttgtagtcgg ggtaagtcgt gcccgccccg atcgcggtga cgacgatgtc gctctgatgg
     3841 acgatcttgc cgtcccagtc gccggtcgcc tggaatggaa cgacgcgtcc accgtgcgac
     3901 atggtgcgct cgagcacgat gtggggatcg acacgaacca gcttgccgtc gtgattggcg
     3961 tagcggtcgc aggcgcccgc agcgcccggc ttgatgtagc acatcaccgg acacgcatcg
     4021 cagcggatct tgtccccgct cgaggcgctc gtcgcatcag tcaccatgtc aaagccttgc
     4081 aactcagcat cgactatcga acgacggcta atcggcaacg accgacttat tgttcgtata
     4141 cgaatgatcc tgcgcgcgaa gtccacgcct gtcaagcagt gccgtgcgcg aaaagctgcc
     4201 gctgccgaat aatagctcaa ttgcccaggc cggctgttca taaaccgggc aaacggttgc
     4261 ggcgcgtcat gcggtgcttg cacgtttgta tacaaacggt agtcttttcg tgccgcctgc
     4321 cgtcgcgatc gtcggaacca gaacaggatg acgcaaagca tgatccgcct gaccgtgaat
     4381 ggcaggatcc atgacgttgt tgccgctccc gacacggcgc tgctctatgt gctgcgcaac
     4441 gacctcgcat tgaacgggcc gaaatatggc tgcgggctcg gcgaatgcgg gacctgtacc
     4501 gtgctgatcg acggagttgc ggcgcgctcc tgcgtgatcc cgatctcggg ctgcatcgac
     4561 cgcgacatca tgacgctgga aggactcggc acacgcgaac gtcccgatcc cgtgcaggac
     4621 gccttcatca aggaacaggc cgcgcaatgc ggctattgcc tcaacggcat gatcatgacg
     4681 acgaaagcgc tgctcaaaac caatccgcac ccgaccgagg ccgaaatcgt cgaagcgctc
     4741 cgctataatc tttgccgctg cggcgcgcat gtcgaaatca tccgcgcggc gatgcgggcg
     4801 tcgggtcggc tggccgaggc ccgggattga tggtgcaagt tccgcaggga tcgctgtccg
     4861 tcgtgcagtc cgccggcacg gcaacaagct tcgaggcctt tatcaagatc acagccgagg
     4921 gttcggtcac cgcctacaac gggcatgtcg atcttggtac ggggatccgc accgcgctcg
     4981 gacaaatcgt cgccgaagag ctcgatgtct cctttgcgcg cgtcgttgtg gtgctcggag
     5041 atacagcgca ggtgcccgac cagggcgcaa cgatcgcgag cgaaaccatc cagatcacgg
     5101 ccgtgccgtt gcgcaaggcc gcggcgcagg cccggcagtt cctgatcgcg cgcgctgcgg
     5161 aacggcttga agttggtgtc gacgatctgt cgatcgagga tgggctcgtg cgcggtccga
     5221 acaaccgcag cgtcacctat ggcgagttga tcggcgacga cacgatccgc ctcgaacttg
     5281 ccgacgacgt tcccgtgaaa gccgtcgacg gctactccat tgtcgggaaa tcggtgcctc
     5341 gcgtcgatct cccggcgaaa gccacgggcg aactcgtcta tgttcacgac gtccgcgtgc
     5401 ccggcatgct gcacggccgc gtggtgcgcc caccctacgc cggcgtcgat gccggccctt
     5461 ttgtcggcac cagcctgatt gccgtcgatg aagcctcagt acgtcatatc cccggcatgg
     5521 tcgccgtggt caggatcggc gatttcgtcg gcgtcgtcgc agagcgcgag gaaaacgccg
     5581 tgaaggccgc cgcccagctc aaattgagct ggaaaccgac gccaacactg cccgatctca
     5641 aggacatcga gacggcgctg cgcgccaatc cgtcgacgcc gcgaaccctg atcgacaagg
     5701 gcaatgtgga tgccgcgatc gga
//
LOCUS       DATLJT010000669         1501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2798873,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000669 DATLJT010000000
VERSION     DATLJT010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798873"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="VM910_15540"
     CDS             complement(<1..>1501)
                     /locus_tag="VM910_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI63975.1"
                     /translation="REVWGVVQARRRGRAAARLHVRIVALFSIIAAVPVIVVAVVASV
                     TLHRGLDRLFSQQTQSLIENSLIVADAYVREHVQFVRADSIAIAIELARAKPLFDQSR
                     EQFHQFLAVQASIRGLPAVMLLDKDLNIVDQVDPQINEAFVKPSAEVLAAINDTEPQV
                     AMFLDDNYVAAIIKLRGYNDLYLYIARLLDPRVLAQLRATQASVAQYADLSARQIGIQ
                     IAFGLMYTVIALIVLLSAVWIGLNFANYLVAPIRRLIGAAQVVSTGNLYVQVPTRRSE
                     GDLAQLGETFNKMTQELRTQRDDIVRARDLIDSRRRFTEAVLAGASAGVIGVDAESCI
                     SILNRSAERLIGRTEADALGRPLTDIVPELAGMFAAARSGLQRLVQGQVTINRNGQER
                     NLSVRVTSEQSGAAEGGYVVTLDDITELVTAQRTSAWADVARRIAHEIKNPLTPIQLS
                     AERLRRKYAGMIKEDGGIFQQCTDTIVRQVDDIKRMVDEFSRFARMPKPV"
BASE COUNT          275 a          455 c          494 g          277 t
ORIGIN      
        1 tcaccggctt cggcatgcgg gcaaagcgtg agaactcatc caccatgcgt ttgatgtcgt
       61 cgacctggcg gacgatggtg tcggtgcact gctggaatat gccaccgtct tccttgatca
      121 taccggcgta tttccggcgc aggcgttcgg cggaaagctg gatcggtgtc agcggattct
      181 tgatctcatg cgcgatgcgg cgtgcgacat cggcccaggc ggaggtgcgc tgcgcggtta
      241 caagctcggt gatgtcgtcg agcgtcacca cataaccgcc ctcggccgcg cccgactgct
      301 cgctcgtcac tcgtaccgac aggttgcgct cctggccatt gcgattgatc gtcacttgac
      361 cctgaaccag tcgctgcagt ccggagcgcg cggccgcaaa catgccggcg agctcgggga
      421 cgatatcggt cagcggccgc ccgagtgcgt cggcttcggt gcgtccgatc agtcgttctg
      481 cggaccgatt caaaatgctg atgcagctct cggcgtcgac accgatgacg ccggcgctcg
      541 ccccggccaa cacggcttcg gtgaaacgac ggcggctatc gattaggtca cgcgcacgta
      601 cgatgtcgtc gcgctgggtc cgcagctcct gcgtcatctt gttgaaggtt tcgcccagct
      661 gggcgaggtc gccctcggag cgtcgggtgg gcacttgaac gtagagattg ccggtcgaga
      721 ccacctgagc tgcgccgatc aggcggcgga tcggggccac caggtagttg gcgaagttca
      781 gtccaatcca caccgccgac agcagcacga tgagcgcgat caccgtgtac atcaatccga
      841 atgcgatctg aatgccgatc tggcgtgccg agagatcggc atactgagcg acgctagcct
      901 gggtcgcccg cagttgcgcg agtacgcgcg gatcgagcag gcgtgcaatg tacagataga
      961 ggtcgttgta gccgcgcagc ttgatgatgg cggcgacgta attgtcatcg aggaacatcg
     1021 ccacttgcgg ctcggtgtca ttgatggcgg ccagcacctc ggctgatggc ttgacgaagg
     1081 cctcgttgat ctgtggatcc acctgatcga cgatgttgag gtccttgtcg agcagcatga
     1141 cggccggcag tccgcggatc gaggcctgaa ccgcgaggaa ttgatggaac tgctcgcgcg
     1201 attggtcgaa caagggcttg gcgcgcgcga gttcgatagc gatggcgatg ctgtcggcac
     1261 gcacgaactg gacgtgttcg cggacatagg catcggccac gatcaacgaa ttctcgatga
     1321 gcgactgcgt ctgctgcgag aacaggcggt cgagaccgcg gtggagcgtc acgctggcga
     1381 ccacggcaac cacaatcacg gggaccgcag cgatgatgga gaacaatgcg acgatgcgca
     1441 cgtgcagtcg cgccgccgcg cgcccgcgac gcctcgcctg cacgaccccc cacacctcgc
     1501 g
//
LOCUS       DATLJT010000670         1722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4406394,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000670 DATLJT010000000
VERSION     DATLJT010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406394"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..886)
                     /locus_tag="VM910_15545"
     CDS             complement(8..886)
                     /locus_tag="VM910_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HVI63976.1"
                     /translation="MPAARPGETADPLPVTEVASGLYAHIGAIAVMTRQNEGAIANVG
                     FVVGSNAVAVIDSGGSVREGRRLLAAIRAVTPKPIRYVINTHAHPDHIFGNAAFEGAV
                     VVGHKNLPRALAARGQFYLDTFRRVLGDELMADVKIVPPTQVVEREARIDLGDRVLLL
                     QAWRASHTDNDLTVLDETTGTLFAGDLVFLRHLPVLDGSLKGWLAVIDELAAIPAGRV
                     VPGHGPVAGWPAALEGERTYLQRLAQHVRGLIKRGETIGAASQSVAESERTRWELFDE
                     SNARNATAAYSELEWE"
     gene            1303..>1722
                     /locus_tag="VM910_15550"
     CDS             1303..>1722
                     /locus_tag="VM910_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HVI63977.1"
                     /translation="MTAIIRRFVSCALVILFWAPISASSQDISEPEEYRTEDYRAPTP
                     TTLKGARVVTTAEAEQIWKAGGAIFLDVLPYAPRPTNLPPGTIWREKPRRNIPGSSWL
                     PDTGYGALAPVTENYLRTNLARVTGGDRTKALVVYCLR"
BASE COUNT          358 a          548 c          486 g          330 t
ORIGIN      
        1 aacaatccta ttcccattcg agctccgagt aagccgcggt ggcgttgcgg gcgttgctct
       61 cgtcgaatag ctcccaccgc gtccgttccg attccgcgac gctctgcgac gcggcgccga
      121 tcgtctcgcc gcgcttaatc aaaccacgga catgctgcgc caaacgttgg aggtatgtgc
      181 gttcaccctc aagggccgcc ggccagcccg cgaccggacc gtggcccggc acgacgcgtc
      241 cggctggaat ggctgccagt tcgtcaatga ctgcaagcca acctttcagg ctcccgtcga
      301 gaaccggcaa atgccggaga aagaccaggt cgccggcgaa cagcgttccg gtggtctcgt
      361 ccagaaccgt aagatcattg tcggtgtgcg aggcccgcca ggcctgaagc aagaggactc
      421 gatcgcccag atcgatccgt gcctcgcgct cgacgacttg tgttggcgga acgattttga
      481 catccgccat caattcgtcg cccagcaccc gtctaaacgt atcgagatag aactggccgc
      541 gagcagcgag cgctcgtggg aggttcttgt ggccgacgac aacggcgccc tcgaacgcag
      601 cattcccgaa gatgtgatcg ggatgggcat gggtattgat gacgtaccga atcggcttcg
      661 gcgtcactgc gcggatggct gcaagcagtc ggcgcccttc gcgcacgctt cccccgctgt
      721 caatcacagc gacagcgttg ctgccgacga caaatccgac gttggcgatt gcgccctcgt
      781 tctgccgtgt catcacagca atggcaccga tgtgggcata aagacccgac gcgacctcgg
      841 tcacgggcag cggatcggcc gtttcgcccg gccgagcagc cggcattaca gccaggagcg
      901 tcgctatcaa ggtcaaacat gcggtcgcgt tccaccgatt ggctgattga aaagtcactt
      961 tgccatctcc cgtatgagtt ccgaacgact tccacaatgc gtcagtatcg cgacgaacat
     1021 ctcctgattt tccagccgca ccctttcatt ttcaaatgca cctctcattt tcagttgcac
     1081 ccccaaaaac acggacacag aagtcagcgg actcataccg gttgttggag gcacgggaaa
     1141 gtccgggccg ggtatgcgac ccttcgatca gaatggaagc gcgagctgaa cccattgata
     1201 ggagcaatca gcctgagacc aataaccttt tcttgagtta tggtttgcgt tgctcgacga
     1261 gagaaatcag cccatcagga agactccttt gaccaagaac ccatgacagc catcatccgc
     1321 cgtttcgtca gctgcgcgct tgtcatcctc ttttgggcgc ctatctcggc gtctagccaa
     1381 gacatatccg aaccggagga ataccggaca gaagactatc gtgcgccgac acctaccact
     1441 ctcaagggcg cgcgcgtggt cactacggcc gaagccgagc agatctggaa ggcgggcggc
     1501 gccatctttc tcgatgtcct gccgtatgcg ccgcgcccca ccaacttgcc gcccgggacg
     1561 atctggcgag agaagccgcg acggaatatt cccggcagca gctggctacc cgataccgga
     1621 tatggggcgc tcgcgccggt gaccgagaac tacttacgaa ccaatctcgc gcgcgtgacc
     1681 ggaggcgacc gcaccaaggc actcgttgtc tattgtctgc gc
//
LOCUS       DATLJT010000671         1651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_729306,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000671 DATLJT010000000
VERSION     DATLJT010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729306"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VM910_15555"
     CDS             <1..467
                     /locus_tag="VM910_15555"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63978.1"
                     /translation="PKRERAAKAAEYLDLVHLSRFADLYPGQLSGGMKQRVALARLFA
                     LDPEIMLMDEPFGALDSQTRELLQEELQSIWRRARKTALFVTHDIDEAIYLGTRVIVF
                     TARPGRIKADIRIPNLDRSGDYRKSREYLALRVEVWDLLRDEVLKARALEEA"
     gene            464..1240
                     /locus_tag="VM910_15560"
     CDS             464..1240
                     /locus_tag="VM910_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63979.1"
                     /translation="MRLNSASIQNWILPVAALAAWEILGRAGLLPRYLSTPSAILAAL
                     WEIALTGELFVALAASLYRVSVGFALGTAAGIVVGLGAGMLPGVRHFFDPLVSFLYSI
                     PKVAFLPIFLLLFGLGHASKIAIIAFSGFFPVFVASRHAVLSVDKLLIWAARNMGAPS
                     RTIFFRVVIPAAAPQLFGGIRIGLAHAFVVLFAAELIGSKAGLGTIISYGEEWVRFDL
                     MFAGIVCFAVLGFLSDRMLLAARSRLLKGQMIGTEEEIVR"
     gene            1237..>1651
                     /locus_tag="VM910_15565"
     CDS             1237..>1651
                     /locus_tag="VM910_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine transporter subunit"
                     /protein_id="HVI63980.1"
                     /translation="MTVQIFAQARSLIGRWYSIPLLLVVWQVAVASGLVESRLLPSPE
                     KVWSALVTEVGNGTLIYHAGVTVSRALAGFLLAALVGIPFAGAMGRSSLVRNLFEPIF
                     FIGYPIPKIALFPVFTYIFGIGTPSKIAFTFLECLY"
BASE COUNT          239 a          502 c          511 g          399 t
ORIGIN      
        1 tgccgaaacg cgagcgtgcc gccaaggcgg ccgaatatct cgatctcgtt catctttctc
       61 gtttcgccga tctctatccc ggccaactga gcggcggaat gaaacagcgc gttgccctgg
      121 cacggctgtt cgcgctcgat ccggaaatca tgctgatgga cgagccgttc ggcgcgctcg
      181 attcccagac tcgggaattg ctgcaggagg agctgcagtc gatctggcgg cgcgctcgca
      241 agacggcgct gttcgtgacc cacgacatcg atgaggcgat ctatctcgga acgcgcgtga
      301 tcgtgtttac ggcgcgtccc ggccgcatca aggccgacat ccgcattccc aacctcgatc
      361 gttccggcga ctaccgcaag tcgcgggaat acctcgcctt gcgggtcgaa gtctgggacc
      421 tcctccggga cgaggtattg aaggcgcgcg cgctggagga agcgtgagac tcaactcggc
      481 ttcgatccag aattggattt tgccggtcgc ggcgttggcg gcctgggaaa ttctcggccg
      541 cgccggccta ctgccgcgct atctgtcgac gccgagcgcg atccttgcgg ctctgtggga
      601 aatcgcgctc acgggcgagc tcttcgtcgc gctggccgcg agcctctatc gggtgtcggt
      661 cgggttcgcg ctgggcacgg ctgccggcat cgtcgttggc cttggcgcgg ggatgctacc
      721 cggtgtgcgc catttcttcg atccgctggt atcctttctc tactccatcc cgaaggtcgc
      781 gtttctgccg atctttctgc ttctgttcgg cctcgggcat gcatccaaga ttgccatcat
      841 cgcattttcg ggcttctttc cggtgttcgt ggcctcgcgt cacgcggtgc tgtccgtcga
      901 caagcttctc atctgggcgg cgcgcaatat gggagccccc tcgcggacga tctttttccg
      961 cgtcgtcatt ccggcggccg cgccgcagtt gttcggcggt atccgcatcg ggctcgcgca
     1021 tgcctttgtg gtgctgtttg cggccgagct gatcggctcg aaggctgggc tcggtacgat
     1081 catctcctac ggcgaggaat gggtgcggtt cgatttgatg ttcgccggta tcgtctgctt
     1141 cgccgtgttg ggatttttga gcgaccgcat gttgctggcg gcccgatcgc gcctgctcaa
     1201 gggccaaatg atcggcacgg aggaagaaat cgtacgatga ccgttcaaat cttcgcgcaa
     1261 gcgcggtcgc tgattgggcg ctggtactcg atcccgctgc tccttgtggt ttggcaagtg
     1321 gcggtcgcga gcggtctcgt ggaatctcgt ctgcttccga gcccggagaa ggtttggagc
     1381 gcgcttgtga ccgaagtcgg caatgggacg ctcatctatc acgcaggcgt gacggtttcg
     1441 cgcgctctcg ccggcttctt gctcgcggcg ctcgttggaa ttccattcgc cggtgccatg
     1501 gggcggtcct cactcgtgcg caatctgttc gagccgattt ttttcatcgg ctatccaata
     1561 ccgaagatcg ccttgtttcc cgttttcacc tatatattcg gcatcggaac gccttcgaag
     1621 atcgcattca cttttctcga atgcctctat c
//
LOCUS       DATLJT010000672         6152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4177344,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000672 DATLJT010000000
VERSION     DATLJT010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177344"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VM910_15570"
     CDS             <1..994
                     /locus_tag="VM910_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI63981.1"
                     /translation="PEFTEQIWQYINRRVSDWRIIHGKMALKKNAALFDRIEKDFGVE
                     RGTLLALWGVESAYGDPLVQQSHMRPVFPSLAALAWNEPRRKAYWEQELINALRIVDR
                     GWSMPEQMRGSWAGAMGHTQWMPEVWLNVGIDYDGDGKISPFGKPDDALGSTAKYLVN
                     RGNYHRGEHWGYEVRGANGGSGKKRTYAAWASAGVTRADGQAFRQPNASARLWIPVPG
                     GPAFLLGPNFYAVKTYNPSMNYALAICHLGDRILGAPPFHQPFPDSERALTLAEVQEM
                     QTRLTKAGFDTGGTDGRVGNDTMKAVRDYQLKMGLSPADGYGGLKVLDRLRQGS"
     gene            complement(991..2832)
                     /gene="recJ"
                     /locus_tag="VM910_15575"
     CDS             complement(991..2832)
                     /gene="recJ"
                     /locus_tag="VM910_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HVI63982.1"
                     /translation="MTLPASALPAEAPPAFLGVTQSVTGKLWRDRLDARGAARALAIA
                     QRYQLPEMLSRVLAGRDVAIEDVPDFLDPTIRKLMPDPFTVTQMEIAAKRIADAALRG
                     EKVAIFGDYDVDGATSAALLAWHLRHCGLDPLIHIPDRLFEGYGPNTEAVRALAAKGA
                     TLLIAVDCGTTSVEPLAEAKRLGLSVVVIDHHQCGVELPEVDALVNPNRPDDLSGLGY
                     LAAVGLTLVTLVAVNRELRARGFWTGEMPEPDLLGMLHHVALGTVADVAPLIGLNRAF
                     VAKGLIAMRRRDHVGHTALMDVSRLSGPPEAWHLGFMLGPRINAGGRIGRADLGVRLL
                     LEADVSEAARIAAELDRLNSERRLIEQAAEAQAEAEAFASLGLEDKGSVIVTASEGWH
                     PGVVGLVASRLKEKFGRPAFAIALEPGGIGTGSGRSISGVDLGKAVRQAVADGLLLKG
                     GGHAMAAGVTLRKERLAEFRAYMESMLAHDVAQSRHANELFIDGAVTARGATPEFVAM
                     LNRAGPFGSGNPEPVVALPSHQLIFADEVGQAHLRVRFKSGDGAILNGIAFRAVGQKL
                     GQALAQHREQVLHVAGSLAVDRYQGSERVQLRVLDVAVPDRGPSVIR"
     gene            complement(2909..3628)
                     /locus_tag="VM910_15580"
     CDS             complement(2909..3628)
                     /locus_tag="VM910_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVI63983.1"
                     /translation="MSDLSAVKALVFDVFGTVVDWRTSLINDFTKWGAARGINADWTA
                     LVDGWRAVYAASMDVVRKNPQDGYVILDVLHRRSLEKLVEQFSIKGLTDADLHHLAMG
                     WHRLHPWPDSVAGLTRLKAKYIISPLSNGNVALLTNMAKFAGLPWDLIMSAELFEHYK
                     PDPETYLGAASLLCLKPEQVMMVAAHNHDLKAAQKQGLKTAFVARPTEYGPLQKYDFE
                     AKGDWDIVAKDFGGIADRLGC"
     gene            complement(3644..4645)
                     /gene="glpX"
                     /locus_tag="VM910_15585"
     CDS             complement(3644..4645)
                     /gene="glpX"
                     /locus_tag="VM910_15585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HVI63984.1"
                     /translation="MSTHISVPPQLLLERILTLEIVRVTERAAVSAARLRGHGNEKAA
                     DQAAVDAMRRELNKLPIEGTVVIGEGERDEAPMLFIGEKVGLNAGPKVDIAVDPLEGT
                     TLCAKNMPGAIATMAMADGGTLLHAPDVYMQKIAIGPGYQKGVVELDASPADNVCRLA
                     KAKGVEPGAITVLVLDRPRHADIIQGVRSTGAAVRLITDGDVAGVIHTASPEETGVDM
                     YIGTGGAPEGVLAAAALRCIGGQMQCRLILDTDEKRERAHKMGIADPRMIYGIEDMVK
                     GDCLFAATGVTTGSLLSGVKFRKDNVYETETVVMRSVTGTVRYIKAEHRQLEKFHLD"
     gene            complement(4727..6046)
                     /locus_tag="VM910_15590"
     CDS             complement(4727..6046)
                     /locus_tag="VM910_15590"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVI63985.1"
                     /translation="MVAPLRVGIAGLGTVGAEVVRLIENEGRVLAARCGRSVRVVAVT
                     ARSKSKKRAIDLNGIAWAKNPLALANDPGVDCLVELIGGSGEPALSAIETALKAGKSV
                     VTANKALIARHGLRLAHAAEKHGGALNFEAAVGAAIPVVKTLREGLAATGVNRVYGIL
                     NGTCNYILTRMEQEGLSFAECLKDAQRLGYAEANPSFDIDGHDTAQKLAILASLAFGT
                     KVAQSAVYVEGISSITPEDLRAASDLGYRVKLLGVAVKTAKGIEQRVHPTMVPKSSSI
                     AQVMGVTNAVTIDGEGIPPITLVGPGAGGAATASAVLADIADIARGVRVNPFGRPVER
                     LRATQKAPMERHEGGYYIRLMARDLAGTAATIATRLAEQKISIESIVQRHPNGVDVAQ
                     AAGKASPVPVLLITYATHEDAVHRALAAVQRDKVISGRPQVIRIEKN"
BASE COUNT         1142 a         2037 c         1868 g         1105 t
ORIGIN      
        1 gcccgagttc accgagcaga tctggcaata catcaaccgc cgcgtctccg actggcgcat
       61 catccacggc aaaatggcat tgaagaagaa tgcggcgctg ttcgatcgca tcgaaaagga
      121 tttcggcgtc gagcgcggca cattgctcgc gctctggggc gttgaatccg cctacggcga
      181 tccgctggtt cagcaaagcc atatgcgtcc ggtttttccc tcgctcgcgg cgctcgcctg
      241 gaacgagcca cgccgcaaag catattggga gcaagaactc atcaacgcgc tgcgcatcgt
      301 cgaccgcggc tggagcatgc ccgagcaaat gcgcggctca tgggccggcg cgatgggtca
      361 cacgcaatgg atgccggaag tctggctcaa tgtcgggatc gactatgacg gcgacggcaa
      421 gatttcaccc ttcggcaagc ccgatgatgc cctgggttcg accgcaaaat atctggtcaa
      481 tcgcggcaat tatcaccgcg gcgaacattg gggctacgag gtgcgcggtg caaacggcgg
      541 tagcggcaaa aagcgaacct atgccgcctg ggccagcgcc ggcgtcaccc gcgccgatgg
      601 tcaggccttt cgacagccga atgcgtcggc acggctttgg ataccggtgc ccggcggtcc
      661 tgccttcctg ctcgggccga atttttatgc ggtgaaaacc tacaacccgt cgatgaacta
      721 tgcgctcgcg atctgccacc tcggcgaccg tatcctcggc gccccgcctt tccaccagcc
      781 gttcccggat tcggagcgcg cgctgacgct tgcggaagtc caggagatgc agacgcgcct
      841 gacaaaggcc ggcttcgaca ccggcggcac cgacggccgc gtgggcaacg acaccatgaa
      901 ggcggtccgc gactatcagc tcaaaatggg gctttcgccc gccgacggct acggcggact
      961 gaaagtgctg gaccgattgc ggcagggcag ctagcggatt accgacgggc cccgatcggg
     1021 caccgcaacg tcgagcacac gcaattgcac acgctctgat ccctgatagc ggtcgaccgc
     1081 aagcgagcct gccacgtgca agacctgctc gcgatgctga gccagcgcct ggccgagctt
     1141 ctgaccgacc gcgcgaaatg cgatgccgtt gaggatggcg ccgtcgccgg acttgaaccg
     1201 tacccgcaaa tgcgcttggc ccacctcgtc ggcaaagatc agttgatgcg acggcagcgc
     1261 gacaaccggc tcggggttgc cactgccgaa gggccccgcg cgattgagca ttgcaacgaa
     1321 ctccggcgtt gcgccgcgcg cggtcacggc gccatcgatg aagagctcgt tcgcatggcg
     1381 tgattgagca acatcatggg ccagcatgct ctccatatac gcacgaaatt ccgccaatct
     1441 ctccttgcgc aacgtgacgc ccgccgccat cgcgtggccg ccacccttca acaacaagcc
     1501 gtcagcgacc gcctggcgca cggccttgcc aagatcgacg ccggaaatcg agcggcccga
     1561 gccggtgccg atgccgcccg gctcgagcgc gatcgcaaag gcaggtcgcc cgaacttttc
     1621 cttcagccgc gacgcgacca ggccgaccac gcctggatgc caaccctccg acgccgtcac
     1681 aatcacgctg cctttgtctt ccagcccaag cgaagcgaac gcttccgcct cggcttgcgc
     1741 ttcggcggcc tgctcgatca accgccgttc gctattgagc cggtcaagtt cggcggcaat
     1801 gcgcgcggct tccgacacat cggcttccag caaaagtcgc acgccgagat cggcgcgtcc
     1861 aattcgcccg ccggcattga tgcgcggccc cagcatgaag ccgagatgcc aggcctccgg
     1921 cggaccgctc aaccgcgaaa cgtccatcag cgcggtatgg ccgacatggt cgcgccgacg
     1981 catcgcaatc aggcctttgg cgacgaaagc gcggttgagt ccgatcaacg gcgcgacatc
     2041 ggcgactgtg ccgagcgcaa catgatgcag catgccgagc agatcgggct cgggcatttc
     2101 gcccgtccaa aatccccgcg cgcgtaattc gcggttcacc gcaacgagcg tgaccagagt
     2161 gaggccgacg gctgcgagat agccaaggcc ggacagatcg tccggccggt tcggattgac
     2221 cagtgcgtcg acctccggca gttcgacgcc gcattggtga tggtcgatga caacgacgga
     2281 caggccaagc cgtttggctt cagcgagcgg ctcgacgctc gtggtgccgc aatcgactgc
     2341 gatcaggagt gtggctcctt tcgcggccag cgcccgcaca gcctcggtat tcggtccata
     2401 accttcgaac aggcggtcgg gaatatggat caagggatcg agcccgcaat gccggagatg
     2461 ccaggcaagc aacgccgcgg acgttgcgcc gtcgacatcg tagtcgccga atatcgcaac
     2521 cttttctccg cgcagcgcgg cgtcggcaat gcgctttgcc gctatctcca tctgcgtcac
     2581 tgtgaacgga tccggcatga gcttgcggat cgtgggatcg agaaagtcag gcacatcctc
     2641 gatcgcaaca tcgcggccgg ccagaacacg cgagagcatc tccggcagtt gataacgctg
     2701 tgcgatggcg agcgcgcggg cagcaccccg ggcatcgagc cggtcgcgcc aaagctttcc
     2761 ggtcacggac tgcgtcacgc caagaaacgc cggcggcgcc tctgccggaa gtgctgatgc
     2821 gggaagcgtc atagcggtcc caagataggg agcgcgaggc agattcgcga ccttttcatc
     2881 ctccggcttg cgggatgcgg ttggaaaact agcaccccag gcggtcggct atgccgccaa
     2941 aatccttcgc cacgatgtcc cagtcgccct tggcctcgaa atcgtatttc tgcagcggcc
     3001 catattcggt ggggcgcgcc acgaaagcgg tcttcagacc ctgcttctgt gcggccttga
     3061 ggtcatggtt gtgggccgca accatcatca cctgctccgg cttcagacag agaaggcttg
     3121 ccgcgccgag ataggtttcg ggatctggct tgtagtgctc aaaaagctcc gccgacatga
     3181 tgaggtccca gggcagacct gcgaacttcg ccatgttggt caagagcgcg acgttgccgt
     3241 ttgagagcgg cgagatgatg tattttgcct tcagccgcgt caggccggcg acgctgtccg
     3301 gccagggatg caaccggtgc cagcccatcg cgagatgatg gaggtcggcg tcggtcagtc
     3361 ccttgatcga aaattgctcg accaacttct ccagcgagcg gcgatgcagg acgtcgagga
     3421 tgacatagcc gtcctgagga ttcttgcgca caacatccat cgaggcggca tagaccgcgc
     3481 gccagccgtc gaccagcgcg gtccagtccg cattgatgcc gcgcgcagcg ccccatttgg
     3541 tgaagtcatt gatcaggctg gtgcgccaat ccaccacggt gccgaacaca tcgaacacca
     3601 gcgctttcac cgcggaaaga tcggacatct cggccttccc ttgtcagtcg aggtggaatt
     3661 tttccaattg ccgatgctcg gccttgatgt agcgcaccgt gccggtgacg gagcgcatca
     3721 ccacggtctc ggtttcatac acgttgtctt tgcggaactt gacacctgat agcagcgacc
     3781 ccgtggtgac gccggtggcc gcaaacaagc agtcgccctt taccatgtcc tcgatgccgt
     3841 aaatcattcg cggatcggca atccccatct tgtgcgcgcg ctcgcgcttc tcgtcggtgt
     3901 ccagaatgag ccggcattgc atctggccgc cgatgcagcg aagcgccgcc gcagccagca
     3961 cgccctccgg cgcgccaccg gtgccgatgt acatgtcgac gccggtctcc tcaggacttg
     4021 ccgtgtgaat cacgccggcg acatcgccgt cggtaatcag ccgcaccgcg gcaccagtcg
     4081 aacgcacgcc ctgaatgatg tcggcatggc gcgggcggtc tagcaccagc acggtgatcg
     4141 cgcccggctc gacgcctttc gccttcgcca gccggcaaac attgtcggcg ggggaagcgt
     4201 cgagctcgac cacgcccttt tggtatcccg gcccgatcgc aatcttctgc atgtagacgt
     4261 cgggcgcatg caacagcgtg ccgccatcgg ccatcgccat ggtcgcaatc gcgcccggca
     4321 tgtttttcgc gcacagcgtc gtaccctcga ggggatcaac cgcgatatcg accttcggac
     4381 cggcattcag gccgactttc tcgccgatga aaagcatcgg cgcttcgtcc cgttcgcctt
     4441 cgccgatcac gaccgtacct tcgatcggca gcttgttgag ctcgcgccgc atcgcgtcca
     4501 ccgcggcctg gtcggctgcc ttctcgttgc cgtggcctcg cagccgggca gccgagaccg
     4561 cggcgcgctc cgtcacgcgc acgatttcca gcgtaagaat gcgctcgagc aacagttgcg
     4621 gcggaacgga aatatgggtc gacattttgc gcactccctc aaacctgttc ggcgaagccg
     4681 tggcccgcca acaacctttc ggcgcgcgat ccgttcggat cgagcgttaa tttttttcga
     4741 tcctgatgac ctgcggccgc ccgctgatca ccttgtctcg ttgcacggca gcgagcgcac
     4801 ggtgcaccgc atcctcatgg gtcgcgtagg taatcagcag caccgggacc ggcgaagctt
     4861 ttcctgctgc ctgcgcaacg tcaacgccat tcgggtgacg ctgcacaatg gattcaatcg
     4921 aaatcttctg ttcggcaaga cgcgtggcga tcgttgctgc cgtgccggcg agatcgcgtg
     4981 ccatcagacg gatgtaatag ccgccttcgt ggcgctccat cggcgccttc tgggtcgcgc
     5041 gcagccgctc gaccggacgg ccgaacggat tgacgcggac gccgcgcgcg atatcggcga
     5101 tgtcggccag aaccgcggag gctgtcgccg cgccacccgc accgggaccg acgagtgtga
     5161 tcggcgggat gccctcgccg tcgatggtga ccgcgttcgt cacccccatc acctgggcga
     5221 tcgaagagga tttcggaacc atggtcggat gaacgcgctg ctcgatgccc tttgcggtct
     5281 tcaccgcaac cccaagcagc ttgacgcggt aaccgaggtc ggacgccgcg cgcaggtctt
     5341 ccggcgtgat cgaggagatg ccctcgacat agaccgcgct ctgcgcgacc ttggtgccga
     5401 atgcaaggct tgccaggatc gcaagcttct gagcggtgtc gtgaccgtcg atgtcgaagc
     5461 tcggattggc ttccgcgtag ccgagccgtt gcgcatcctt gagacattcg gcaaacgaca
     5521 atccctcctg ctccatcctc gtcaggatat agttgcaggt gccgttgaga atgccgtaaa
     5581 cgcggttgac gcctgtagcc gcgagacctt cgcgcagggt cttcaccaca gggatcgccg
     5641 cgccgacggc tgcctcgaaa ttcaatgcgc cgccatgctt ctcggcggca tgcgcgagcc
     5701 gcaagccgtg cctggcgatc agggccttgt tggcggtcac caccgacttg cccgccttca
     5761 atgccgtctc gatcgccgac aaggcgggct cgcctgagcc gccaatcaat tccacgaggc
     5821 aatcgacacc ggggtcgttt gcgagagcca gcgggttctt ggcccaggca attccgttca
     5881 gatcgattgc gcgctttttc gatttcgatc gcgcggtcac cgccaccacc cgcacggatc
     5941 ggccgcagcg cgcggccagc acgcgacctt cattctcgat caggcggaca acctcggcac
     6001 ccacggtgcc gagccccgcg atacccactc tcagtggtgc gaccattacc aggaaacctt
     6061 gcggaagatt tatcgccggt tggcgagcgg aaccacgttg tgcaacgttt cgatgccgct
     6121 ttcaaggaaa cggcgcacac cccgggccgc ct
//
LOCUS       DATLJT010000673         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_41607,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000673 DATLJT010000000
VERSION     DATLJT010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41607"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1272
                     /locus_tag="VM910_15595"
     CDS             322..1272
                     /locus_tag="VM910_15595"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI63986.1"
                     /translation="MKSLAIVTVLGLCAFATDAVAQSWQPPSPNQRCPSKWGAADERG
                     SGNHMKPANVLRAAQLIKTGETFELSHVLGRDMPLNPGRQYDLHTKRSTGPFGANQRY
                     SNEELIISEIGQVGTQFDGFTHQALDRLLYNCVKMDDVASRNGFTKMGLEKVGTLMTR
                     GVLIDIAGLKGVDILPINYEITVADLEQALEKQGIRLQPGDAVLIYTGWSKHWGVDNK
                     LYTSGCPGIGVAAAEWLIKQDPMLLGSDNWPVEIAPNPDKAINLPVHNIALTVNGVHL
                     LENLVLEDLAKARAYEFAFVMQPLKLQGATGSTVAPTAIR"
     gene            1861..>2043
                     /locus_tag="VM910_15600"
     CDS             1861..>2043
                     /locus_tag="VM910_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI63987.1"
                     /translation="MTTVGGPTGRSHRAQAPTSSAPTPSIKHCLNATVRAADGLDAWF
                     RRYVIEHPLRYGASEFR"
BASE COUNT          394 a          645 c          662 g          342 t
ORIGIN      
        1 ttcgaggccg cgctggggag gctcgggctg accgaccgca aagaccccct gacggcggcg
       61 gtcgcgaagg ccatcatcca gcttgccaag gatggcgagc gcgatcccga gcggctgcgc
      121 gacggtgcgc tcaggagcct cggcaagtag gccgccgcag tcggcggcct cggcgttttt
      181 ttatggtcct tcacactgcg ctgtcagccg cgcattagtg caagaggacg ggcacggata
      241 tgatgtagcg ccgcagatcg cacgcgtagg cgtgcgctcc cgcgtatgga tcgatgtcac
      301 accacaaaag ggaggaatgc gatgaagagc ctcgccatcg tcacggtctt gggcctctgc
      361 gctttcgcaa ctgacgctgt cgcccaaagc tggcagccac cgtcgcccaa ccaacgctgc
      421 ccgtcgaaat ggggcgccgc cgacgaacgc ggctcgggta atcatatgaa gcccgccaac
      481 gtgctgcggg cggcgcagct gatcaagacc ggcgagacct tcgaactgag tcacgtattg
      541 ggacgggaca tgccgctcaa tcccggccgg cagtacgatc tgcataccaa gcgcagcacc
      601 ggcccattcg gcgcgaacca gcgctacagc aacgaggagt tgatcatttc ggagatcggc
      661 caggtcggaa cgcagttcga tgggttcacc catcaggcgc tcgatcggct gctctacaac
      721 tgcgtgaaga tggacgacgt cgcgagccgc aacggcttca ccaagatggg gctggagaag
      781 gtcggcacct tgatgacgcg tggcgtgctg atcgacatcg ctggcctcaa gggtgtcgat
      841 attctgccaa tcaactatga gattaccgta gccgatttgg agcaggcgct ggagaagcaa
      901 gggatacgcc tgcagccggg tgatgccgtg ctcatttaca ccggatggag taagcactgg
      961 ggcgtcgaca acaagctcta cacgagcggc tgtccgggaa tcggcgtggc tgcggccgag
     1021 tggctgatca agcaggaccc gatgttgctt ggttcggaca actggccggt ggaaatcgcc
     1081 cctaatccgg acaaggcgat caatctgccg gtccacaaca tcgcgcttac cgtcaacggt
     1141 gtccatctgc tcgagaacct cgtgctcgag gacctcgcca aggcgcgcgc ctacgagttc
     1201 gcgttcgtca tgcagccgct gaagctgcaa ggggccaccg gttcgaccgt agcgccgact
     1261 gcgatccgct gagcgacagg ctcgcgatgt cccgctgacc gaccgcaaag acccactggc
     1321 gacggcggtc gcgagggtaa tcataaagct ggccaaggat ggcgagcgcg atcccgagcg
     1381 ggtgcgctcg ggatgctcgg caagcaggcc gccgcacttg gcggcctcga tttttttgtg
     1441 gtccttcaca ctgcgccgtt catgccgatt gcgtcgttcc cttgggggcc cggtctcgac
     1501 gcttgcgacc cacagcggtc atcggcgaga tcggcatccc gcggtgcacc ggcggtctca
     1561 tgccgcctcg aagggacaac gcaacctgcg cgagccggat gctgcatccg gcgcaacgcg
     1621 cgcatggctg tcggccccat ccgtactttg gaaccgaaga atccgtgctc tggaaccgaa
     1681 gaaagagcac cactggcaag ttccagccaa cgcgcggcga ggctgccttt ataaaaatgt
     1741 cgggaacccg acgcagcggc cgtgcgttac gttgtcggcg cggagcaggg gaccagggcc
     1801 agcccgtgcc gcggcgcgat cgccgcggtg cgagaagcat ggctcaaagg gagcgtctcc
     1861 atgacgacag tcggcgggcc caccgggcga tcgcatcggg cccaagcgcc cacgtcttca
     1921 gcgcccacgc cttcgataaa gcactgtctc aacgccacgg ttcgggcagc cgacggcctc
     1981 gacgcctggt tccgccggta cgtgattgag catccgcttc gctacggcgc gagcgaattt
     2041 cgt
//
LOCUS       DATLJT010000674         1817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1190456,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000674 DATLJT010000000
VERSION     DATLJT010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190456"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VM910_15605"
     CDS             <1..297
                     /locus_tag="VM910_15605"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HVI63988.1"
                     /translation="GDVVYKVAPSFWSEQHDMYIQGVGWPVAQPSARIRRPLGAGAAL
                     LFELDGTRLAHALGINAQRDMAVVRRLIERGVPVDASELADAGKPLAAMLKAKV"
     gene            347..1189
                     /locus_tag="VM910_15610"
     CDS             347..1189
                     /locus_tag="VM910_15610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI63989.1"
                     /translation="MTTGKKERVGIVGIGRMGLAMLKHLVKHGYQVTACDIDDKQLAK
                     ARDAGAATAETPAEVARAADFVIIGVGYDDEVNQVVLGTDGLLAALAPGAVIAVSSTA
                     KPDTVKSLDERARAAGVAILDAPICRGRFAADDGTLLALVGGKPDVVERGRAIYRCFC
                     SDYAHLGDVGHGQVGKTMNNLLLWINAVGLIEAGRLAETTGIDLGKLRAALLMSSGAS
                     DALKEWDRISFTWALKDMQIVADLTDKAGLSLPITGAIKELVKDARRIKATNAPKWTG
                     VGDR"
     gene            complement(1248..>1817)
                     /locus_tag="VM910_15615"
     CDS             complement(1248..>1817)
                     /locus_tag="VM910_15615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI63990.1"
                     /translation="TADVSKGSDVAALVRAVVERFGRIDILVNNVAISDNKHIFDITE
                     EEWDRVLAVTLKSQFLMGKHVAQQMAAQGTGGRIVNIGSTSGFMGRSRALAYSAAKGG
                     VANLTRAMAAQLAPHKIRVNAIVPNKIGSPVGKDEFDPSRPVPNMAKRPGQPLEAAKA
                     VLFLASEDSSFVYGANLFVDGGVSAMDLS"
BASE COUNT          323 a          595 c          594 g          305 t
ORIGIN      
        1 ggcgatgtcg tctacaaggt cgcgccctcg ttctggtcgg agcaacacga catgtatatc
       61 caaggcgtgg gctggcccgt tgcacaaccg agcgcgcgca tacgccggcc gctcggcgcg
      121 ggcgccgcgc tcctgttcga gctcgacggc acccgcctcg cgcacgcgtt gggcatcaat
      181 gcgcagcgcg acatggcggt ggtgcggcgg ttgatcgagc gcggggtgcc ggtcgacgcc
      241 agcgagctcg ccgatgccgg caagccgctc gccgccatgc tcaaggcgaa ggtatagccg
      301 cgtcggcgag cggaaccacg agacctacgg cgcataggtg acatccatga caaccggaaa
      361 gaaggagcgc gtgggcattg tcggcatcgg ccgcatgggc cttgcgatgc tcaagcatct
      421 cgtcaagcac ggctaccagg tgaccgcctg tgacatcgac gataaacaac ttgccaaggc
      481 gcgcgatgcc ggcgccgcca ccgccgagac tccggccgag gtcgcacgcg cggccgattt
      541 cgtcatcatc ggcgtcggct atgacgacga agtcaatcaa gtcgtgctcg ggacggacgg
      601 gttgctcgcc gctctcgcgc ccggcgcggt catcgcggtg tcctcgaccg ccaagcccga
      661 caccgtcaag tcgctcgacg agcgcgcgcg ggcggcgggc gtcgcgatcc tcgatgcgcc
      721 gatctgccgc ggccgcttcg ccgccgatga cggaacgctg ctcgcgctcg ttggcggcaa
      781 acccgacgtg gtcgagcgcg gacgcgcgat ctaccggtgc ttttgctccg actatgccca
      841 tctcggcgat gtcggccacg gccaagtcgg caaaaccatg aacaacctgc tgctttggat
      901 caatgccgtg ggcttgatcg aagcgggccg gctggccgag accaccggga tcgatctcgg
      961 caagctgcgc gcggccttgc tgatgagctc aggcgcctcc gacgcgctca aggaatggga
     1021 caggatctcc ttcacttggg cactcaagga catgcagatc gtcgccgacc tgaccgacaa
     1081 ggccggcctg tcgctgccga tcaccggcgc catcaaggaa ttggtcaagg acgcgcgccg
     1141 catcaaggcg acgaatgcgc cgaaatggac gggagtcggg gatcggtaat cagtaatcag
     1201 aaaaagcgcc cgcatctgat tactgaccac tgatgactga ttcctggtca cgacaaatcc
     1261 atcgccgaga cgccgccgtc gacgaacaga ttggcgccgt agacgaacga agaatcctcg
     1321 ctcgccagga acagcaccgc tttggcggcc tccagcggtt ggcccgggcg cttggccatg
     1381 ttcggcactg gtcgcgaggg atcgaactcg tccttgccga ccggggagcc gatcttgttt
     1441 ggcacgatcg cgttcaccct gattttgtgg ggcgcaagtt gcgccgccat ggcgcgcgtc
     1501 aaattcgcga ccccgccctt ggcggccgaa taggcgaggg cgcgacttct gcccatgaag
     1561 ccggaggtcg agccgatgtt gacgattcgc ccgcctgtgc cctgcgccgc catctgctgg
     1621 gcgacgtgct tgcccatgag gaactggctc ttgagcgtga ccgccagcac tcggtcccac
     1681 tcctcctcgg tgatgtcgaa aatgtgcttg ttgtcggaga tcgcgacgtt gttgaccaga
     1741 atatcgatgc ggccgaagcg ctccaccacc gccctgacca gggcggcgac gtcgctgccc
     1801 ttggagacgt cggcggt
//
LOCUS       DATLJT010000675         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4406880,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000675 DATLJT010000000
VERSION     DATLJT010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406880"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1585
                     /locus_tag="VM910_15620"
     CDS             8..1585
                     /locus_tag="VM910_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HVI63991.1"
                     /translation="MNKFERQSRKAADQETLPDAIEAELAEIPAQVIAVRDAPPIAPR
                     SPLTQAEVRTILMSLMVTMFLAALDQTIVATALPTIGRQFHDVSNLSWVITAYLLAST
                     AVAPVFGTLSDIYGRRAMIIAALSLFIAGSALCALAPSMPVLILARGLQGLGGGGILP
                     IVQTVISDLVSPRERGQYQAYFSGVWVAAGVGGPVLGGVFAEHLHWSMVFWINLPLSF
                     AALGLLLPKMGRIPTFHRKRKVDWLGGVLLMASAVTFMLVLTWGGNRFVWLSPAILAM
                     VGASIALALSFVWHAARADEPFLPLSLMRGSVVPFAIIAGGCALGSMTGLTVHLPLYY
                     EVVYHLTASQAGLALIPLAAISTCGAAIAGRTMVHARHYKRVAIVGDLIAAVSGAALA
                     LATLPLWGLLVLLSVFALGLGTTFPISVVSIQNAVARSQVGTVTGAMNFFRALMSSFT
                     VAAFTAVLLMALGADISLAGEHRGPVNSIPVADMITAFRYVFGAAAAMMGCAALCMIV
                     MEERPLAGPSRSVKMAE"
     gene            complement(1642..2034)
                     /locus_tag="VM910_15625"
     CDS             complement(1642..2034)
                     /locus_tag="VM910_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI63992.1"
                     /translation="MSVLVLVVDDEPDVEALFRQQFRRDLRTQRFMMDFAHSAADALS
                     RIASTIGQSLILILSDINMPGMTGLEMLPKVKEMRPEVPVIMITAYGDAETKRKALES
                     GAEGLLTKPIDFALLREEIDSRLAQAAS"
     gene            complement(2184..>2677)
                     /locus_tag="VM910_15630"
     CDS             complement(2184..>2677)
                     /locus_tag="VM910_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI63993.1"
                     /translation="NLAYHGARAEKPQFNVTLMRNFDPEAGMIEVFPQEITRVLLNLI
                     SNAFYAVTKRKAENGSADFEPLVTATTKGRTDRVEISIRDNGTGIPPDVKEKMFNPFF
                     TTKPAGEGTGLGLSMSHDIVVKQHGGTIEAATEPGEFTEFTIVLPRTSAAVLKSASSS
                     ASS"
BASE COUNT          428 a          824 c          842 g          583 t
ORIGIN      
        1 aatcctgatg aacaagttcg agcggcagag ccgtaaggct gccgatcaag agacattgcc
       61 ggacgccatc gaagctgagc ttgccgagat cccggcgcag gtgattgcgg tccgcgacgc
      121 acccccgatc gcgccccgct ccccgctgac ccaggccgaa gtccgcacca tcctgatgag
      181 cctgatggtg acgatgttcc tggccgcgct cgaccagacc atcgtcgcaa ccgccttgcc
      241 gaccatcggc cgccagttcc acgacgtctc caacctgtcc tgggtgatca cagcctacct
      301 tttggcgtcg accgcggtcg cacccgtgtt cggcacgctg agcgacatct atggccgccg
      361 cgccatgatc atcgcggcgc taagcctgtt catcgcgggc tccgcgctct gtgcactcgc
      421 gccaagcatg ccggttctga tcctcgcgcg cggccttcag gggctcggcg gcggcggcat
      481 cctgccgatc gtgcagacgg tgatttcgga cctggtcagc ccgcgcgagc gcggccagta
      541 ccaggcctat ttcagcggcg tttgggtcgc ggccggcgtc ggcggcccgg tgctcggtgg
      601 cgtatttgcc gaacacctgc actggtcgat ggtgttctgg atcaacctgc cattgtcgtt
      661 tgcggcgctc gggctgctgt tgccgaagat gggcaggatc ccgacgtttc accgcaagcg
      721 caaggtcgat tggctcggcg gtgtgctgct gatggcgtcc gccgtgacgt tcatgctggt
      781 gctgacctgg ggcggcaacc gctttgtctg gctgtcgccg gcgatccttg cgatggtggg
      841 cgcgtcgatc gcgctggcgc tgagcttcgt gtggcatgcc gcacgtgcgg atgagccttt
      901 cctgccgttg tcgctgatgc gcggcagcgt cgtgccgttc gcaatcattg ccggcggttg
      961 cgcgctgggg tcgatgaccg ggcttacggt gcacctgccg ctctattacg aggtggtgta
     1021 tcacctcacc gccagccaag ccggactcgc gctgattcca cttgctgcga tctcgacctg
     1081 tggcgcagcg atcgccggcc gcaccatggt gcatgccagg cactacaagc gcgtcgcgat
     1141 tgttggagac ctgatcgctg ccgtcagcgg cgccgcgctt gcgctcgcca ccctgccgct
     1201 ctggggcctg cttgtgttgc tgtcggtgtt cgcactcggg ttagggacga catttccgat
     1261 cagcgtggtg tcgattcaga acgcggtggc gcgctcgcag gtcggcactg tcacgggagc
     1321 catgaacttt ttccgtgcgt tgatgtcctc gttcacggtg gccgccttta ccgccgtgct
     1381 gttgatggcg ctgggcgccg acatttcgct tgccggcgag catcgcgggc cggtgaactc
     1441 gattcccgtc gcggacatga tcacggcgtt tcgttacgta ttcggcgcgg ccgccgcgat
     1501 gatgggatgc gccgcgctct gcatgatcgt gatggaagag cggccgctgg ccggcccctc
     1561 gcgctccgtc aagatggcgg agtagttggc ggcgacacga ctgcgccacg ggaaatcagg
     1621 tgcggcctcg ctcaaaccgc atcagcttgc tgcctgcgcc agccgcgagt cgatttcctc
     1681 gcggagcagc gcgaagtcga tcggcttggt caagagtcct tccgcaccgc tttcgagcgc
     1741 cttgcgcttg gtttcggcgt caccgtacgc cgtgatcatg atcacgggga cctcgggccg
     1801 catctccttg accttgggga gcatttcgag cccggtcatt cccggcatgt tgatgtcgga
     1861 caaaataagg atcagcgatt gcccgatcgt gctggcgatg cgcgacagcg cgtccgccgc
     1921 cgaatgggcg aaatccatca taaagcgttg ggtgcgcaaa tcgcggcgga attgctggcg
     1981 gaacagtgcc tcgacatcag gctcgtcatc taccacaaga acgagaacgc tcacgtctaa
     2041 cctctgattt tggtttgaaa gaattttgcc agtgtcccga ttccggaatt cgcatcatct
     2101 gttgcggtat cctcatttgc gaacttcgga atcgggaccc agtaacttat tgatccagtg
     2161 tggctttggt tcagaaatca gcatcacgaa cttgcggacg acgatgcgga cttcaaaacc
     2221 gccgcactgg tacgcggcaa tacaatggtg aactcggtaa attcgcccgg ctcggtcgcg
     2281 gcctcgatcg tgccaccgtg ctgtttcaca acgatgtcat ggctcatcga gagcccgagc
     2341 ccggtgcctt cgcctgcggg cttggtcgtg aagaacgggt tgaacatctt ttcctttaca
     2401 tccggcggaa tgccggtgcc gttgtcgcgg atgcttattt cgacgcggtc ggtgcgacct
     2461 tttgtcgtcg ccgtaaccag aggctcgaag tcggcagacc cgttctcggc cttgcgcttg
     2521 gtcacggcgt aaaaagcgtt tgagatcaaa ttcagcagaa cgcgggtgat ttcctgcgga
     2581 aacacttcga tcatgcctgc ctcgggatcg aaatttcgca tgagcgtgac gttgaattgc
     2641 ggtttttcgg cgcgcgcgcc gtgataggcc aaattga
//
LOCUS       DATLJT010000676         3904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1650411,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000676 DATLJT010000000
VERSION     DATLJT010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650411"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VM910_15635"
     CDS             complement(<1..827)
                     /locus_tag="VM910_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI63994.1"
                     /translation="MRREGSAFSGLGVVMLKELSDHLTSIRMRVLEWLVVLVAVAALY
                     GAIQQIKDTTAEDPFLFLRLFTTSRDPLPSFVSFLSFLVPLMAIGLGFDAVNSEHNRR
                     TLSRILSQPIYRDALLFGKFLAGLVTLSISLITLWLLVIGLGLFLIGIPPGSEEIARS
                     VVFLLVTIVYVGVWLALALLFSILFRSAATAALVTLGLWLLVTFIWPVLSGALAQIIV
                     PPDPRYTALGLQTPGTAQLEQILARFSPSTLYAEIVVALLDPTTRALGPIYLSQLQGA
                     "
     gene            complement(817..1785)
                     /locus_tag="VM910_15640"
     CDS             complement(817..1785)
                     /locus_tag="VM910_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI63995.1"
                     /translation="MIADAVIEANGLAKTYGSAFAVDHISFGVGRGEIFGLLGPNGAG
                     KTTTILMLLGLTEISEGEVRVLGFNPAREPLSVKRRVGYLPDTVGFYDNLTAADNLRY
                     TARLIGFRPMEREKRIAAALDRAGLAEFADNRVGTFSRGMRQRLGLAEIVMKGAQIAI
                     LDEPTSGLDPHATAELLGIIRGFKSEGVSVLLSSHLLERVQSVCDRVALFNDGRIALM
                     GSVAELGRQVLGGGYVVEIEAEGSGLAERLALIPGVGAVEKAGINKLRLQADRDVRPE
                     AAAEVVAMQGRLKYLGVQEPSLEAIYTRYFEAAPEQAAQERTRHAA"
     gene            complement(1782..2972)
                     /locus_tag="VM910_15645"
     CDS             complement(1782..2972)
                     /locus_tag="VM910_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HVI63996.1"
                     /translation="MRYGLIAAVAAAIAVLTAPLTIPSGSRAFAEDAPKDVKGIFLMS
                     DYPAVTLRPGETSTINLRLQNYNMTPERLALTLSGVPAGWTATLLGGGQPVAAAMPAA
                     NSSVSLSLRLDVPKNAPMGTQTVTVAAQGNTTNLQLPINVTLAKDLPAKLTLTPQLPD
                     LRGTSKSSFEYQLGIKNDSGKKLTVSLSAQAPQNFDTSFTEQYGTQELKAVPVDAGQS
                     KDVKLKVTPPNTAAAGDYKVVARVAAEDAAATADLTMTITGQPKLDISGREGLVSTRA
                     SAGKETTVPIIITNTGTAPAEDIQLNGSAPSGWKITFDPKTVDRIAPNDHKEVQALIT
                     PTDKAIAGDYVTTVRASARGESASTNFRVTVTTSTMWGIAGVGIIGVALLVMVGAVAR
                     FGRR"
     gene            3147..3626
                     /locus_tag="VM910_15650"
     CDS             3147..3626
                     /locus_tag="VM910_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI63997.1"
                     /translation="MPRRTQISAGILVFRRKPELEVLLAHPGGPFWAKKDAGAWTIPK
                     GLVEPGDEPIATARREFTEETNLVAEGDMIALVPVAQKSGKLVHAFVLEADFDLSQFA
                     SNMFTAEWPPKSGRRESFPEIDRIAYFALPLAKLKITAYQRPLLAELEARIGLTRRS"
     gene            complement(3642..>3904)
                     /locus_tag="VM910_15655"
     CDS             complement(3642..>3904)
                     /locus_tag="VM910_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004127020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVI63998.1"
                     /translation="CRSKYGSGGPFLFGSFGAADAMFAPVVWRFHTYAVEVSSTALAY
                     MRAMMALPPWNEWRDAARRETWVLAEDEVDWPQVLREEPSRG"
BASE COUNT          804 a         1230 c         1163 g          707 t
ORIGIN      
        1 gcgccctgca attggctcag ataaatcggg ccgagcgcac gcgtggtggg atccagcaag
       61 gcgaccacga tctccgcata gagcgtggaa ggagaaaagc gcgccaggat ctgctccagt
      121 tgcgcggtgc ccggcgtctg caacccaagt gcggtatagc gcggatccgg cggtacgatg
      181 atttgcgcga gcgcgccgga aaggaccggc caaataaacg tcaccaggag ccataggccc
      241 agtgtcacca gtgcagccgt ggcagcggag cggaacagaa tcgagaacaa taacgcaagt
      301 gccagccaaa ccccgacata gacgatggtg accaggagaa atacgacact gcgggcaatt
      361 tcctctgagc ccggcggaat cccgatgagg aaaagaccga gcccgatcac cagaagccag
      421 agcgtgatca ggctaatcga aagcgtgact aggccggcca ggaattttcc gaacaacagc
      481 gcgtcgcgat agatcggctg cgacagtata cgcgacagcg tgcggcgatt gtgctcgcta
      541 ttgacggcat cgaagccaag tccgatcgcc atcagcggca cgaggaacga aagaaaggag
      601 acaaaggacg gcaacgggtc gcgcgatgtc gtgaacaacc gcaggaacag gaacggatct
      661 tccgccgtcg tgtccttgat ttgctgaatc gcaccataga gcgcggcgac cgccaccagc
      721 acgacgagcc attccagcac acgcatgcgt atgctggtca ggtggtccga aagctccttg
      781 agcatgacca cgccgagccc ggaaaaggcg gaaccttcac gccgcatggc gggtcctctc
      841 ctgcgcagcc tgctccggcg ccgcctcaaa ataacgcgta tagatcgcct ccaggctcgg
      901 ctcctgcacg ccgagatatt tcaggcgacc ctgcatggcg accacctcgg ccgccgcttc
      961 tggccgcacg tcgcggtcgg cctgcaggcg aagcttgtta atgccggcct tctcgactgc
     1021 gccgacgccc ggaataagcg cgagccgctc ggccaacccg ctgccctcag cctcgatctc
     1081 gacgacgtag ccgcctccga gcacctgccg gccaagttcg gcgaccgagc ccatcagcgc
     1141 gatgcgccca tcattaaaga gcgcaacccg atcgcagacg ctctgtaccc gctccagcag
     1201 atgcgaggag agcagaaccg aaacgccctc gctcttaaaa ccgcggatga tgcccagaag
     1261 ctccgccgtg gcatgcggat caagtcccga tgttggctca tcgaggatcg cgatctgtgc
     1321 acctttcatt acgatctcgg ccaagccaag ccgctggcgc atcccgcgcg aaaaggtacc
     1381 gacgcggttg tccgcgaatt cggcgagccc ggcacggtcc agcgccgcgg cgatccgctt
     1441 ttcccgttcc atcggccgga acccgatcag ccgcgcggta tagcgcagat tgtccgccgc
     1501 agtgaggttg tcatagaagc cgaccgtatc gggcaagtac ccgacccttc gcttgaccga
     1561 aagcggctcc cgcgccggat tgaacccgag tacgcgaacc tcgccttcgg aaatttcggt
     1621 caggcccagc aacatcaata tcgttgtcgt cttgccggcg ccatttggtc cgagcaaccc
     1681 aaaaatctcg ccgcgtccga cgccgaatga aatgtggtcg acggcaaacg ccgacccgta
     1741 ggttttagcg aggccgttag cctcgatcac ggcatctgca atcatcggcg accgaacctc
     1801 gcaaccgcgc caaccatcac caacagcgca acgccaatga tgcccactcc cgcgatcccc
     1861 cacatggtcg aggttgtgac ggtgacgcgg aaattggtcg atgccgattc gccgcgcgca
     1921 gatgcgcgga cggttgtcac ataatcgcca gcgatggcct tgtccgtcgg cgtgatcagc
     1981 gcctgcactt ctttgtggtc gttcggagcg atgcggtcga ccgttttcgg atcgaaggtg
     2041 atcttccatc cgctcggcgc cgatccgttc agctgaatgt cttccgcagg cgccgtgccg
     2101 gtattggtga tgatgatggg gaccgtagtc tccttgcccg ccgaggcgcg ggtggacacc
     2161 aggccctctc ggccgctgat gtcgagtttc ggctgcccgg tgatggtcat agtcagatcg
     2221 gccgtcgccg cggcgtcctc cgccgccacg cgcgctacga ccttgtagtc accggctgca
     2281 gccgtattcg gcggagtgac tttgagtttg acgtccttgc tttggccggc atcaaccggg
     2341 actgccttta gttcctgggt cccgtactgt tccgtaaacg aagtgtcgaa attttgcggc
     2401 gcttgcgcgg acaagctgac ggtcagtttt ttaccgctgt cgtttttgat gccgagctga
     2461 tattcgaagc tcgatttcga ggtgccgcgc aagtccggaa gctgcggcgt gagcgtgagc
     2521 ttcgcgggca gatccttggc gagcgtcacg ttgatcggca gctgtaaatt cgtggtgttt
     2581 ccttgcgcgg ccacagtgac ggtttgcgtc cccatcggcg cgttctttgg cacatccagc
     2641 cgcaatgata gcgagacgct cgagtttgcc gctggcatcg ccgcggcaac cggctgaccg
     2701 ccaccgagca aggtcgccgt ccagccggcc ggcacgccac tgagcgtgag cgccaggcgc
     2761 tcaggcgtca tgttatagtt ttgtaaccta agattgatcg tggaggtctc gccggggcgc
     2821 agcgtcaccg ccggataatc gctcataaga aaaatgcctt tgacgtcttt aggcgcatct
     2881 tcggcaaagg cgcgcgaccc cgatgggatc gtgagcggtg ccgttaaaac ggcaatggca
     2941 gccgcaacag cggcgattaa cccatatcgc attggcatag ctcccgtgca ggcaagtgcc
     3001 ctaccccgtc gcacaaagcg cgctcgggcg gggaatgggc attctcggcg caatcgtgac
     3061 aaaatcatgc agacgctggc ctatttgaca agctctgtcg cagatgtcct gcggtagaga
     3121 gccgcagtca atgggagcca agctttatgc cgcgccgaac gcaaatcagc gccgggatac
     3181 tggtgtttcg ccgcaagcct gagctcgaag tcctgctcgc tcatccgggc ggcccgtttt
     3241 gggcaaagaa ggatgccggc gcctggacca tcccgaaagg cctagtcgaa cccggcgacg
     3301 aacccatcgc cacggcacgg cgcgaattca ccgaagaaac aaatctcgtc gctgaaggtg
     3361 acatgattgc acttgtgccc gtcgctcaaa agagtgggaa actcgtgcac gccttcgtcc
     3421 tggaggcaga cttcgacctt tcgcaattcg ccagcaacat gtttacagcg gagtggccgc
     3481 ccaaatcagg gcgaagagaa agttttccgg aaatcgatcg gatcgcttat ttcgcgctgc
     3541 cgctggcgaa gctcaagatc actgcctatc agcggccgct gctggccgaa cttgaagccc
     3601 gtatcggcct gacccgccga tcgtgacggg catcgtggtt gctatccgcg cgacggctct
     3661 tcgcgcaaaa cctgcggcca atcgacctcg tcctccgcca gaacccaggt ctcgcgccgc
     3721 gcggcatccc gccattcatt ccatggcggc agcgccatca tcgcgcgcat ataggcgagc
     3781 gcggttgagc tcacctcaac tgcgtaagtg tgaaatcgcc acacgacagg agcaaacatg
     3841 gcgtcggcgg cgccgaatga gccaaacagg aatggaccgc ccgagccgta tttgcttcgg
     3901 catt
//
LOCUS       DATLJT010000677         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3258276,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000677 DATLJT010000000
VERSION     DATLJT010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258276"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VM910_15660"
     CDS             complement(<1..405)
                     /locus_tag="VM910_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVI63999.1"
                     /translation="MRLDVPIKRHETDVLIVGGGAAGTMAAFECAEAGVDVIQATKGR
                     ATSGTTTVARGGFAAAMGADDSPELHLHDILKYGGELIDPELARVWVYDIIDVVHDLE
                     SWGAEFVRRADGRLDLKEFPSHSRNRACHHYDT"
     gene            complement(437..1684)
                     /locus_tag="VM910_15665"
     CDS             complement(437..1684)
                     /locus_tag="VM910_15665"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HVI64000.1"
                     /translation="MSASRPIIAVLATMDTKSSEARFVADVLTRAGAAPWIVDLSMKS
                     HTLSAGDVSGAQVAEAVGVSWQALDERSRQEAAAVMVQGGTRILLERYSKGEIAGAIG
                     VGGANGTNLVCAILRALPYLLPKIMISTVAGTAAVQWYVAESDIAMYPSIGDVSLNRI
                     TRAVMENAANAVAIAAANWTARREAKAAHAPLVAVSSFGGTAACVERVSQRLQALGYE
                     VILFHASGVGGKSLEQLASSGELAGVVDVTTHELADLVVDGVYSAGAARMTGAGAAGL
                     PQVIVPGAIDHSNFWVGQVPERYKTREFFQYNAQNLLMRTNAEEFRQLGAEFARRVNA
                     ARGPVRVLIPVGGFSEHTKRRARDLEGNDKGPWKIPRDYRIFVDSLKRDLTSTLIEEL
                     PLHINDNAFADACVDAFFVIANL"
BASE COUNT          472 a          801 c          784 g          526 t
ORIGIN      
        1 ggtgtcgtag tgatggcagg ccctgtttct ggagtggctg ggaaattcct tgagatcgag
       61 ccggccgtcg gcgcgacgga cgaattccgc tccccacgat tcaagatcgt gcaccacgtc
      121 gatgatgtcg tagacccaga cacgggccag ctcgggatcg atcagctcgc cgccgtattt
      181 gagaatgtca tgaagatgca gctcagggct gtcatccgca cccatcgcag cggcaaagcc
      241 gccgcgcgct accgtggttg tgccactggt ggcgcgccct ttggtcgcct ggatgacatc
      301 gacgccggcc tcggcgcact cgaatgcggc catggttccg gcggcgccgc caccgacgat
      361 caggacgtcg gtctcgtgtc gtttgattgg aacgtcgagc cgcattgggt tgctctgctt
      421 cctgctcttt gttgcatcag aggttagcga ttacgaaaaa tgcgtcgaca caagcgtcgg
      481 caaatgcgtt gtcgttgatg tgcagcggca gttcctcgat taacgtagac gtcaagtcac
      541 gtttgaggga atcgacaaat attctgtagt cgcgcgggat tttccacggc cccttgtcgt
      601 tgccttcgag atcccgtgcg cggcgcttgg tatgctcgct gaagcccccg actggaatga
      661 gaacgcgcac cggacctctg gccgcgttga cccgtctggc gaattccgcg ccaagctgcc
      721 tgaactcctc ggcgttagtg cgcatgagca ggttctgcgc attgtactgg aagaattcgc
      781 gcgtcttgta ccgttcgggc acttggccca cccaaaaatt ggaatgatcg atcgctccag
      841 gcacgatcac ctgcggcaag ccggcagcgc cggcgcctgt cattcgcgca gcgcccgccg
      901 aatagacgcc atccactacg agatccgcaa gctcatgtgt tgtcacgtcg acgacgccgg
      961 caagctcgcc ggaggatgca agctgttcga gcgatttgcc gccgacgcct gaggcgtgaa
     1021 acaggatgac ctcataaccc aaggcttgca gccgctgact gacccgttca acgcacgccg
     1081 ccgtgccgcc gaacgacgag accgcgacga ggggcgcatg agcggccttc gcctcccgtc
     1141 tcgccgtcca gttggcggct gcaatcgcca ccgcgttcgc ggcattctcc atgaccgccc
     1201 tagtaatgcg gttgagcgag acgtcgccaa tcgaagggta catagcgatg tcgctctctg
     1261 cgacatacca ttgcacggcg gccgtgccgg ccaccgtgct gatcatgatc ttcggcaaga
     1321 ggtagggcaa tgcgcgcagg atcgcgcaca cgagattggt cccgttcgca ccgccgacgc
     1381 cgatggcgcc tgcaatttct cccttggagt acctctcaag caaaatcctg gtgccacctt
     1441 ggaccatcac tgctgctgct tcttggcgcg agcgttcatc gagcgcctgc caagacacgc
     1501 ccactgcctc agcaacctgc gcaccggaaa cgtccccagc agacaaggtg tgcgatttca
     1561 ttgacaggtc gacgatccac ggcgccgcac ccgcacgtgt cagcacgtcg gcaacgaagc
     1621 gggcttcgct gcttttcgtg tccatcgttg ccaggactgc gatgatcggt cttgatgcgc
     1681 tcaagaactc cccacgagcc gccctcctac tcaaccatcc gatgacacta acagattcca
     1741 ccgctagagg aagttgaccg acgtcatgga aggaaggcgc caagcttact ggtgccttct
     1801 ttcaactgat cgtgtgtccg gtcaagcggt gagtgacctc agggatgaaa caactgccgc
     1861 cggctcgctc atcggcctac acctcggcca cgaacgggtc tttgctttcg cgaaagcgaa
     1921 gtgaaggcat gggacaatgc tttgaagtcc tgaggctggc cgccgcattt tgtaagggtg
     1981 cggacgccag tttcatgcta tgttttgtgt ccgcggttgg tggtcgtctg ctgccgtggg
     2041 ctttctgctt gtttcactat cgtgccgggc tgggtacagc ctctcagccc aggcttcact
     2101 tagtgggtaa gtgccattga ggcttgcgat ggccagcgag cgtcgccgca gtcgtctatt
     2161 gccggatgag catgcaccga gtgcgtcgcc agtaccggtc cgcccgccgg ccgcggggtc
     2221 atcggcctgg agcgacctcc gcggcggtca gtttgcggcc gcgatcccag cggtagggcc
     2281 tacggacgtc gggcgtgggc taaagtcttt ggtcgcaaga cgcgggccgc gggcgactaa
     2341 agcgcgcgcg gccgcgagca ggggcatggc gctcgcccga acgatcagct ccgttccgag
     2401 actcgccttc gccaaaaggc ggttcgtcga tcacatcgtc tgattgggtg atcggctccc
     2461 gatgtgggcg gaacagcttt agttccggaa cgactagcgt cctcagagcc gtgtttgcca
     2521 gccgagatga accaatggct tcggcacccg cgttgccaga agcaaccgtt tcaggacagt
     2581 tcg
//
LOCUS       DATLJT010000678         3475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_960036,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000678 DATLJT010000000
VERSION     DATLJT010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960036"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(555..1649)
                     /locus_tag="VM910_15670"
     CDS             complement(555..1649)
                     /locus_tag="VM910_15670"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI64001.1"
                     /translation="MKNVITYVGIDAHKKDLFIAMLIGTQETSVTWTVPNETQAVRRL
                     VRKLERAAPGPVKCYYEAGPCGYTLQREMTTERVSCHVVAPALIPRKPGERVKTNRRD
                     ARKLGELARAGLLTEVRPPTPEEEAVRDLCRARDDAREDLQRCRHRLGKLLLRRGLHF
                     PGRNWTRAHRQWIERIEWGHAPTGVVANDYLLAIDQLEARLTELDAALTAIAETAPYA
                     EPVGWLRCFRGIDTLTAILLLAELHDFRRFHDPRALMAYVGLVPGEHSSGEKHRRGRI
                     TRTGNALVRRLLVETAWHYQHRAGIGPALTRRRKGQPSRVIAIADKAQQRLCRRFRRL
                     ADHHKPAPKVAVAIARELAGFLWAALQPAK"
     gene            complement(1785..3287)
                     /locus_tag="VM910_15675"
     CDS             complement(1785..3287)
                     /locus_tag="VM910_15675"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI64002.1"
                     /translation="MKRRRRERRHASTHPPGPVIPSESDPVAEIERLRRENEHLRKQV
                     ADQAREIDDLKRQLALRQQNSTTTSKPPSSDGLAGKQRARGRRVKSRRKPGGQPGHPG
                     HHRKLLPPEQVDTIVDHVPTQCSRCARRLRGRDTVGQPRRHQVTELPPIAAHITEHRC
                     HRRQCPDCGTITVAPLPDDCSSQFGPQLTALIAYLTVGCRLPRTVVQQLLQGVLQISI
                     SVGSTQNAWEEASTAVAAPYAELQQALPHQAVLNADETGHRTNGDKRWLWTFVAQTFV
                     FYTIATSRGSGVLRELLGKTFAGILGSDRLPSYLKYAAAQRQFCWAHLKRNLLSALEL
                     ARTATARRFCREALVLERRLFRLWHRYRGDPAARGAPLTRAELIAHVLPIEKKLFALA
                     ERHVNAADANVRNLARALFMNHRHFFTFVNQEGVSPTNNASERALRPAVIWRKLYFGT
                     RSEAGEIAIARLLTITRTCQLQQLNVLVYITAAIAAHRRRQRVASLLTKR"
BASE COUNT          601 a         1143 c         1054 g          677 t
ORIGIN      
        1 agcggacgat gctccatcct ccgtcacctc ttcgatcagt cctcgccgac gcctgatcat
       61 ctggtcactc taacctgata tggaatgacc tcttgtcaag ccgcctcttt tcgaagctgg
      121 gttggccctc ggccccgcaa gcgtcagctt actggcatac gcgcatcctc gtcagagaat
      181 gagcgcgggg tccaggagcg ccgaaggcgc cgcctgtagg gcgcgaagcc ctggaggccg
      241 ggctcattct ctgatatcgg cgacgcacca cgccgtgcgc ggaccgcgaa gacgacggac
      301 aatcgagggc gcttcgacga cgagtcacgc tgatattcgg tggttaccac ggttgcccgc
      361 ggcagagcac acatgacagt gcttcgtcgg gaggacgtgc agtctaaggt cgcgagttgg
      421 gacagctgtc cgtatcgcat ggttagcccg ccgatcctcg caatcgtgcc tctgatcatc
      481 cgccgtcttc gcggtccgtc acggccccgc gtcctctcct ttcggccatc cgagcgttgc
      541 tttcgacgtc cccgctactt cgccggttgg agcgccgccc agagaaagcc cgccagctcg
      601 cgcgcgatgg ccaccgcaac tttcggtgcc ggcttgtggt ggtccgccaa acgtcggaac
      661 cggcgacaca atcgctgctg tgccttgtcc gcaatcgcga tcacgcggct cggttgccct
      721 ttgcggcggc gcgtgagcgc cggcccaatc cccgcccgat gctggtagtg ccacgcggtt
      781 tccaccaaca gtcgccgaac cagcgcattc ccggtgcgcg tgatgcgacc gcgtcggtgc
      841 ttctcgccac tcgaatgttc gcccggcacc agccctacat atgccatcaa cgcccgtggg
      901 tcgtgaaagc gccgaaaatc atgcagctcc gccaggagca ggattgcggt taacgtgtcg
      961 atgccccgga aacatcgcaa ccacccgaca ggttcagcat agggcgccgt ctccgcgatc
     1021 gccgtcagcg ccgcatccaa ttcggtgagc cgcgcctcga gttgatcgat cgccagcagg
     1081 taatcgttcg ccacgacgcc tgtcggagcg tggccccatt cgatgcgctc gatccactgg
     1141 cgatgcgcgc gcgtccaatt gcgccccgga aagtgcaagc cgcgccgcag cagcaatttg
     1201 cccaagcgat ggcggcagcg ctgcagatct tctcgcgcgt cgtcgcgcgc ccggcacaga
     1261 tcacggactg cttcttcctc cggtgtcggc ggccgaacct ccgtcagcag acccgcgcgc
     1321 gcgagctccc ccagtttacg cgcgtcccgc cgattggtct tgacccgctc gcccggcttg
     1381 cgcggaatca gcgccggcgc cacgacgtgg caactcacgc gctccgtcgt catttcgcgt
     1441 tgcaacgtat agccgcacgg ccccgcttcg taatagcact tgacgggtcc cggcgccgcg
     1501 cgctcgagtt tgcgaaccag acggcgcacg gcctgtgtct cgttgggaac tgtccacgtc
     1561 accgatgtct cttgcgtccc gatcagcatc gcgatgaaga gatctttctt gtgcgcgtca
     1621 atcccgacgt acgtgataac gttcttcatg accggctccc ttcgatgcgg ctctggcgcc
     1681 atttggcgct aacccgcgat ctggtggctc tgtccaccgt gttgagtgct gcaagcacta
     1741 cgacgttacg atggagagcc ggtcattccg taactgttca gggattatcg ctttgtgaga
     1801 agcgaggcaa cacgctggcg acgacggtga gcggcgatcg cagctgtgat atagacgagt
     1861 acattgagct gttgtagctg gcaggtccgt gtgatcgtca gtaagcgagc aatcgcgatc
     1921 tcgcccgctt cactacgagt cccaaaatac agttttcgcc agatcacagc tggcctgagt
     1981 gccctctcac tcgcattgtt ggttggggag acgccctcct ggttgacgaa ggtaaagaag
     2041 tgccgatgat tcatgaagag ggcacgagcc agattccgca cgttcgcatc agcggcattg
     2101 acatggcgct cggccaacgc aaagagcttc ttttcgatcg gcaacacatg tgcgatcaat
     2161 tctgctcggg tgagcggagc gccgcgcgct gctggatcac cgcgatagcg atgccacagc
     2221 cggaacaggc gccgttccaa caccaaggct tcgcggcaga agcgtcgcgc cgtcgccgtg
     2281 cgggccagct ccaacgcgct cagaaggttc cgttttaagt gagcccagca aaactggcgc
     2341 tgcgccgcgg catatttcag atagctgggc agtcggtcgc tgccgaggat ccccgcgaac
     2401 gtcttgccga gcagctctcg cagcacgccc gacccgcgcg aggtcgcgat ggtatagaac
     2461 acgaacgtct gcgcgacgaa cgtccacaac caccgtttat cgccattggt ccgatggccc
     2521 gtttcatcgg cattcaacac ggcctggtgc ggcaacgcct gttgcaattc ggcgtagggc
     2581 gcggcgacgg cggtgctggc ttcctcccaa gcgttctgcg tgctgccgac actgatggaa
     2641 atctgaagca cgccttggag caactgctgg acgacggtcc gtggcaaacg gcatccgacc
     2701 gtcagatagg caatcagcgc cgtcagttgc gggccgaact ggctgctgca gtcatcgggg
     2761 agtggcgcca cggtgatcgt cccgcagtca gggcactggc gccgatggca gcggtgctcc
     2821 gtgatgtgcg cggcgatcgg cggcagctcg gtcacttgat ggcggcgggg ctgccccaca
     2881 gtatcgcgcc cgcgcagacg gcgggcacag cggctgcact gagtcggcac atgatcgacg
     2941 atcgtgtcca cctgttccgg tggcagcagc ttccgatgat gcccgggatg tccgggctgt
     3001 ccgcccggct tgcggcggct cttcacgcgc cgtccgcgcg cccgttgctt acccgcgagg
     3061 ccatcggagg acggcggttt cgatgtggtt gtggaattct gctgccgtag cgcgagctgc
     3121 cgcttcaaat catcaatctc gcgcgcttga tcggcgacct gtttccgaag atgttcattc
     3181 tcgcgccgca gacgctcgat ctccgcgacg ggatcgctct cagacgggat aaccggtccg
     3241 ggcgggtgcg tgctggcgtg ccgtcgctct ctccggcgcc gcttcacgca tgtgagggta
     3301 ccgaatcacg cgaagaagta caagaggcct gaacagttac ccacggacat tctgcacttt
     3361 cgcgcgcgtg ttctcgctgt ttacgaccat agctacttcg cctagacaag ctaagcattt
     3421 agctcacgcc ggtatcgcgc cggtccacat cctcccaacc ggcgctcatg ttgac
//
LOCUS       DATLJT010000679         3369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2799664,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000679 DATLJT010000000
VERSION     DATLJT010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2799664"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VM910_15680"
     CDS             complement(<1..924)
                     /locus_tag="VM910_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64003.1"
                     /translation="MSKRNPTWISDWNPEDEAFWKSTGKGIARRNLIWSIFAEHLGFS
                     IWLIWSIVATKLPQAGFHYTTDQLFQLVALPGLIGSLIRFPYTFAVTTFGGRNWTIFS
                     AAVLFIPTIALAYFVTQPDTPFWLMLVVAATAGLGGGNFASSMANISFFYPDRMKGWA
                     LGLNAAGGNIGVSGVQLLTPLLLGVGFVSLYQATPMAGGLYLQNAGLMWLLPLTIAVI
                     GAFLFMNNLTSARSSFKDQLVIVRRKHTWVMSYIYIGTFGSFIGYSAAFPLLIKTQFP
                     EVTVAIAFLGPLVGSLSRPLGGWLSDKIGGAI"
     gene            1263..1670
                     /locus_tag="VM910_15685"
     CDS             1263..1670
                     /locus_tag="VM910_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI64004.1"
                     /translation="MTFEGGCYCGKIRYVAQGEPRLKAQCHCRECQYISGGAPNMFML
                     MPPEGFRYLSGTPKAFARSDLEQPVTREFCETCGTHIATRRPGLNAVILKVGTLDDPS
                     LFGAPQMAIYAVDRQPFHIIPESLPIYERLPPR"
     gene            complement(1832..2416)
                     /locus_tag="VM910_15690"
     CDS             complement(1832..2416)
                     /locus_tag="VM910_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVI64005.1"
                     /translation="MQPSLNIVIVDENPIRAAVLEDGLREAGHVHVVRLGETSHLLAR
                     IYALDPDVILIDLENPSRDVLEQMFQVSRAVKRPIAMFVDQSDKASIEAAVDAGVSAY
                     IVGGLRKERIKNILDLCVSRFNSFARLRDELERARTALEERKVIDRAKGILMTAKNLT
                     EEAAYALLRRTAMNESKKIAEVAQSVVTAAELLK"
     gene            2890..>3369
                     /locus_tag="VM910_15695"
     CDS             2890..>3369
                     /locus_tag="VM910_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64006.1"
                     /translation="MRFHADLHVHSKYSRATSRDLDLEHLAAWACRKGIGVVATGDFT
                     HPAWCAELKQKLVPAEPGLYRLRGDIEQAIAQTLPPACRASVRFMLEVEISTIYKKAD
                     RTRKIHHLIYAPDFETVDRMSARLARIGNIASDGRPILGLDARDLLEIALESGPHAYL
                     "
BASE COUNT          701 a         1052 c         1001 g          615 t
ORIGIN      
        1 gatcgcgccg ccgattttgt cggagagcca tccgccaagc ggacgcgaga gcgaacccac
       61 cagcggtccc aggaaggcaa tagcgaccgt gacctccggg aattgggtct ttatcagcag
      121 cggaaacgct gccgagtagc cgatgaagga gccgaacgtc ccgatgtaaa tgtaagacat
      181 cacccacgtg tgcttgcgcc tgacgataac aagctggtcc ttgaacgagg agcgtgccga
      241 cgtgaggttg ttcataaaga gaaacgcgcc gatcaccgcg atggtcagcg gcagcagcca
      301 catcagcccg gcgttctgaa gatagagacc tcccgccatc ggggttgcct ggtaaagact
      361 tacaaaacca acccccagca gaagcggcgt cagcaactgc acgccgctca cgccgatatt
      421 tccgccggct gcgttcaagc cgagcgccca gcccttcatg cggtccgggt agaagaagga
      481 aatgttggcc atgctcgagg cgaagttgcc gcccccgagg cctgccgttg ccgccaccac
      541 cagcatcagc cagaacggcg tgtcaggctg ggtcacaaaa tacgcgagtg cgatcgtcgg
      601 aatgaacagc accgccgcac tgaaaatcgt ccagttgcgg ccgccgaagg tcgtcaccgc
      661 aaacgtgtaa ggaaaccgga tgagggaacc gatcagtccg ggcagcgcta cgagctgaaa
      721 cagttggtcc gtcgtgtagt gaaagccggc ctgcggcaat ttggttgcga cgatgctcca
      781 gatgagccag atcgaaaaac cgagatgctc ggcgaaaatc gaccagatga gattacgtcg
      841 ggcaatgccc ttacctgtgg atttccagaa cgcctcatcc tccgggttcc agtccgaaat
      901 ccaggtcggg ttccgcttgc tcatcagtgt gttcctttcc ggttggggat agcccccgtg
      961 aaccgaagac gacaaacgcc gccccccggc gctcgaaagc gtttctccca gggcggcggc
     1021 gttgcctgtt cagtccacga caagacgcac ggcgccagca gcggcgaccg cgcggcttcg
     1081 gctttacaag cttcgtgcca actgaggctt gcggggataa agtctcaata aatcaatgga
     1141 taagtcgcca aggacggcat ctcgacaccc cgatcggact ggggcggtct gccctgccaa
     1201 aagcattcgt gcaaaaatcc tgtgcagtgc aaagtctcac cacatgcgga ggatttcatc
     1261 aaatgacgtt cgaaggcgga tgctattgcg ggaaaattcg atacgttgct caaggcgaac
     1321 cacgcctgaa agcgcaatgc cactgccgtg aatgtcaata catcagcggc ggagcaccca
     1381 acatgtttat gctgatgcca cccgaagggt tccgctacct tagcggcacg ccaaaagcct
     1441 tcgcgcgcag cgatctggaa caaccggtga cgcgcgaatt ctgtgagaca tgtggcactc
     1501 acattgccac gcggcggcca ggcctaaatg cggtaatcct gaaagtcgga acgctcgatg
     1561 atccgagcct attcggcgct ccgcaaatgg cgatctacgc ggtcgatcga caacccttcc
     1621 acatcatccc cgagagccta ccgatctatg agcgactgcc accgcgctga tgcgcggggc
     1681 atcgtgaaag acgggtagac cgtgttatcg caaatcattc gcgatctacg cgccgcgact
     1741 gactatgaga tcatcgacgt gctgaacggc taggaggcgc tcgtggcggc gagcgggatg
     1801 aatccaatcc tcaacctcgc ttcgctcgcg atcatttcaa cagctccgca gccgtgacca
     1861 ccgactgggc gacctcggcg attttcttgc tctcgttcat cgcggtcctg cgcagcaggg
     1921 cataggccgc ttcttcggtg agattcttcg cggtcatgag aatgcccttg gcgcggtcga
     1981 tcaccttgcg ttcctcgagc gcggtgcgtg cgcgctcgag ctcgtcccgc agacgggcga
     2041 aggagttgaa gcgcgagacg cagagatcga ggatattctt gatgcgttcc ttgcgcaggc
     2101 cccccacgat gtaggcggag acgccggcgt cgacggcggc ttcgatcgag gccttgtcgc
     2161 tctgatcgac gaacatcgcg atgggtcgct tcaccgcccg gctgacctgg aacatctgct
     2221 caagcacgtc gcggctcggg ttctcgaggt cgatcaggat gacgtcgggg tcgagggcgt
     2281 agatgcgagc gagcaggtgc gaggtctcgc cgaggcgcac cacgtgcacg tggccggcct
     2341 cgcgcagccc gtcctccagg accgccgcgc ggatcggatt ctcatcgacg atgacgatgt
     2401 tgagcgaagg ctgcatgggc gcaggcggca attttcaagc tccgtgccaa gctccctcga
     2461 gcgcggattc tcaagcctta tcaattcgtt gggtgccaaa ccgtcggctg cccgaagatc
     2521 cggcggcgcc cccatggtgc aatcttgggc agagcgctca gcaaggatgc agcgccaacc
     2581 cccagcgcgc gaatgtccac agggggtgct ttccgcttgc ggccgaatcg cgcctttata
     2641 tctgcgcgct ggttttcgcc ggatgctccg ccggagcgcc cacgcagctc ggacgaacaa
     2701 ggcgcggctt ggaacaaggc gtggcagtgt ggtggatttg aagttcctgc cattgtagct
     2761 gcaggctcgc ttaggaactt caaatccaaa accacactag aatcataaat ttgctggtgt
     2821 ccctttgatt ccgaagttcg catcagaggg tgccgcaaag gtttgcgaac ttcggaatcg
     2881 ggacaccaga tgcgcttcca cgcggacctg cacgtccact cgaaatactc gcgggcgacg
     2941 agccgggatc tcgatctcga gcatctcgcc gcctgggcgt gccgcaaggg tatcggcgtg
     3001 gtcgccaccg gcgacttcac ccatccagcc tggtgcgccg agctcaagca gaagctcgtt
     3061 cccgccgagc ccggcctcta tcgcctgcgc ggcgacatcg agcaggcgat tgcgcagacg
     3121 ctaccgccgg cgtgccgcgc ctcggtgcgc ttcatgctcg aggtcgaaat ttccaccatc
     3181 tacaagaagg ccgaccgcac ccgcaaaatc catcacctga tctacgcgcc cgacttcgag
     3241 accgtcgatc gcatgtcggc gcggctcgcc cgcatcggta acatcgcatc cgacggccgc
     3301 ccgattctcg gcctggatgc ccgcgacctc ctggagatcg cgctcgaatc cggtccgcac
     3361 gcctatctc
//
LOCUS       DATLJT010000680         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3717967,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000680 DATLJT010000000
VERSION     DATLJT010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3717967"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1627
                     /locus_tag="VM910_15700"
     CDS             122..1627
                     /locus_tag="VM910_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64007.1"
                     /translation="MSSWLSEREQRLVAGAEAISAATPIPTQIVSNGEYLPPPQSETQ
                     KRVERRMLELADVSSKRLGLSRRQFMQTSCGMAAAFLAMNEIYGNVFEVEAAEAHEPE
                     RMLARAQSLAGQFVFDVQTHFVRDDFKHEELLGLAAFASEHWNPKMKEEGVSSLARYK
                     FQNYVKEIYYDSDTSLALLSGAPFDDPSWWLLSNEQIVKAREIINDFAGSRRLLAHSV
                     ITPKQPGWMEEVDKAIAVYKPDSWKSYTIGDPLTPSKYPWRLDDEQVMYPFYEKVAKA
                     GITTICVHKGLLPPDYEKSFAGVWEYATAWDIGKAAKDWPQMNFVVYHSALRPFLELP
                     DRAWAEFESTGRIRWASDLAEIPQKFGVTNVHAELGTSFANSAVAHPKFSAALVGTLI
                     KGMGADHIMWGTDSVWYGSPQWQIEAMRRLEIPEDMQKKYGFPALGDANSATKQLIFG
                     ANAARLYRINLKAADTRMPAYSEDRLASLKSEYELAAKDPSNLRYGYVRAE"
     gene            1680..>2254
                     /locus_tag="VM910_15705"
     CDS             1680..>2254
                     /locus_tag="VM910_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64008.1"
                     /translation="MRYSSFIGLFCIGLFLNGVADAQSPSGVVRIGILNDQSGPYADF
                     GGKTSVDAARMAVEDVGGKVLGKSIEIIIGDHQNKPDVASAIARRWFDVDGVNAVAEL
                     TNSAVALAVQQIAKEQGKITLFTGPATTRLTNEDCSPTGFHWAFDTYSQAVGTARAVV
                     AEGGKSWFLLVADYAFGHQMASDLSKVVKTSG"
BASE COUNT          487 a          629 c          702 g          436 t
ORIGIN      
        1 aggccatttc ctctgaggag tgcttttcgc caaggttgtg agcgaggctg gcctcatcga
       61 gagcggcggc gtcagcaact aacaaccggt cttgttcaac gaggtaaaag ggaggacggc
      121 aatgagtagt tggctcagcg aacgagaaca gcgcctagtg gccggtgctg aagcgatttc
      181 tgccgcaacc cccataccga cacagatcgt gtccaatggg gagtacctcc cgcctccgca
      241 gagtgagaca caaaaacgcg tcgaacgccg gatgctggaa ctggccgacg tcagcagcaa
      301 gcggcttggc ctcagtcgac ggcagttcat gcaaacgagc tgcggcatgg ctgcggcctt
      361 cctcgccatg aacgaaatct atggcaacgt attcgaggtc gaggcggccg aggcgcacga
      421 gccggagcgg atgctggcgc gggcgcagag tttggctgga cagtttgttt tcgacgtcca
      481 gactcatttc gttcgcgacg atttcaagca cgaggagctt ttgggcctgg ccgctttcgc
      541 aagcgagcac tggaacccga agatgaagga agagggcgtg tcgtcgctcg cccgctacaa
      601 gttccagaac tacgtgaagg agatctacta cgacagcgac accagtttgg cgctgctgag
      661 cggcgcgcca ttcgatgatc catcctggtg gctcttgtcc aacgagcaga ttgtcaaggc
      721 gcgcgaaatc atcaatgact tcgcgggatc acgacgattg ctggcgcatt cagtcatcac
      781 gcccaagcag cccggctgga tggaggaggt cgataaggcg atcgcagtct ataaacccga
      841 ttcgtggaag tcctatacga tcggcgatcc gctcacccca tcgaaatacc cttggcggct
      901 cgatgacgag caggtgatgt atccgtttta tgaaaaggtg gcgaaagcgg gcattacgac
      961 gatatgcgtg cataagggcc tgctgccgcc ggactacgag aaatcgttcg caggcgtgtg
     1021 ggaatacgcg accgcatggg acatcggcaa ggccgccaag gactggccgc aaatgaattt
     1081 cgtcgtttat cattccgcgc tacgcccgtt cctcgagttg cctgatcggg cctgggcgga
     1141 attcgaatct accggtcgca tcagatgggc cagcgatctg gccgagatcc cgcaaaagtt
     1201 cggggtcacc aatgtgcatg cggaactcgg cacgtcgttc gccaattcgg ccgtggcaca
     1261 tccgaaattc tcggccgcgt tggtcggcac gctgatcaag gggatgggcg cagaccacat
     1321 catgtggggc accgattctg tttggtacgg gtcgccacaa tggcagatcg aagccatgcg
     1381 gcggctcgag attcctgagg acatgcagaa gaaatatggc ttcccggccc ttggcgatgc
     1441 caacagtgcg actaagcagt tgatcttcgg cgccaacgcg gcccgcctat atcggatcaa
     1501 tctgaaggcg gcagacacgc ggatgccggc ctattccgag gaccggctgg ccagcttgaa
     1561 gagtgagtac gagcttgccg caaaggaccc gtcaaacctg cgttacggtt acgtccgcgc
     1621 cgagtgacac tgatcggggc gaaatatgcc agtactgcac cgccttttgg gagggaccaa
     1681 tgcgatattc gtctttcatt ggcctgttct gcatcgggct atttctcaat ggcgtcgcgg
     1741 atgcacaaag ccccagcggc gtcgtacgga tcggaattct caacgaccaa tccgggccgt
     1801 atgcagattt cggaggcaag acgtcggtcg atgccgcgcg catggccgtc gaggacgtgg
     1861 gcggcaaggt cctcggaaaa tcgatcgaga tcatcatcgg cgatcatcag aacaaaccgg
     1921 atgtggcctc ggccatcgcg cgacgttggt ttgacgtcga cggggtgaat gcggttgccg
     1981 agctgacgaa ttcggctgtt gcgctggcag tccaacaaat cgccaaggag caagggaaaa
     2041 tcacgttgtt caccgggccg gcgacaacac gcctcaccaa cgaggattgc tcgccgacgg
     2101 gctttcactg ggcgttcgac acgtattctc aggcggtcgg cacagcccgt gccgtcgtcg
     2161 ccgagggcgg caagagttgg ttcctgctgg ttgccgacta cgcttttggc catcagatgg
     2221 ccagcgatct cagcaaggtc gtcaagacca gcgg
//
LOCUS       DATLJT010000681         1763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3258528,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000681 DATLJT010000000
VERSION     DATLJT010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258528"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="VM910_15710"
     CDS             <1..1640
                     /locus_tag="VM910_15710"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVI64009.1"
                     /translation="KALLDSMCSSLAVNLVRPVRFAVETFRFGTARLDLRENTTRTTQ
                     ALRALWRATAQDGGEPPEPRSKEWQSWLLAELARTRQGDRALAGMPSEAEDTLATFKL
                     VGEMRCQLDREAFGSFILSMTRHVSDVLGAYVLAKEAGLFLDAAGVEICPVPIVPLFE
                     TVADLRAAPAIIRSLLEIPLVRRSARWQGGVQEVMIGYSDSNKDGGFLTSNWELAKAQ
                     SKLTRVGEESGVAIAFFHGRGGSVSRGGAPTGRAIAAQPAGSIRGRFRVTEQGEVVSF
                     KYANRGTAAYQIELLASSVFEHALKSELEEALIPKAEFDDAIEAISGASHAVYHRLVS
                     DPGLITYFQSASPLAEISLLNIGSRPASRFGGQSLADLRAIPWVFAWAQNRHVITGWY
                     GVGSGLASFLEVRGERGRELLGRMFMQSRLFRLIVDEVEKTLALVDLYIAGQYADLVA
                     DKALRGAIFSTIESEYVLTRDAILRITGSDTIAERFREYRERLNSRLSIINQVNREQV
                     ELLRRFRTAQNKQARQELKSALLLSINCIAAGLGATG"
     gene            1660..>1763
                     /locus_tag="VM910_15715"
     CDS             1660..>1763
                     /locus_tag="VM910_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVI64010.1"
                     /translation="MSFTLIEQARPRLHRSELAVPGSNPSLFEKSAKS"
BASE COUNT          367 a          483 c          518 g          395 t
ORIGIN      
        1 aaaaggcact gcttgactcc atgtgctcat cgcttgccgt caatcttgtg cgcccagtac
       61 gctttgcggt cgagaccttt cggttcggca ccgcgcgtct cgatttacga gaaaatacga
      121 ctcgtacaac ccaagctctg cgagcactgt ggcgtgcgac agctcaagac ggcggcgaac
      181 caccggagcc cagatccaag gaatggcaat cgtggttgct cgctgagctc gcccgcacgc
      241 gtcaaggtga tcgcgcgcta gccggaatgc ctagcgaagc tgaagatacg ctggcgacgt
      301 tcaagctggt tggcgagatg cgttgccagc tcgatcgcga ggcgttcggt agttttattc
      361 tgagcatgac ccgtcacgtt tcggacgttc tgggtgccta cgtcttagcc aaggaggcgg
      421 gcctcttcct cgatgcagct ggcgtcgaga tctgtccggt accaattgtg ccgttgtttg
      481 agaccgttgc ggacctccgc gctgcaccgg cgatcatacg ctcgctccta gagattccgt
      541 tggtcagacg cagcgcgcgt tggcaggggg gcgtccagga agtcatgatc ggttattcgg
      601 attccaataa ggacggagga ttcttgacgt ccaactggga actagccaag gcgcagtcga
      661 agttaactcg ggttggtgag gaatcagggg tcgcaatcgc tttttttcac ggccggggcg
      721 gttcggttag ccgagggggc gcgccgaccg gccgcgccat tgctgcgcag ccagcaggat
      781 ctatccgggg ccgtttccgc gttaccgaac agggcgaagt ggtctcattc aaatacgcga
      841 accgtggcac tgccgcctat caaattgaac ttcttgccag cagtgtgttt gagcatgcgc
      901 tgaaatccga gctcgaagaa gcgctcattc cgaaagctga attcgacgat gccatcgaag
      961 cgatatcggg cgcttcccac gctgtctacc accgtttagt gagcgatcca ggtcttatta
     1021 cctactttca atcggcaagt ccgctggcgg agatttcatt gctaaacatt ggctctcggc
     1081 cggccagccg gtttggcgga caaagcttag ccgatctgcg cgctatcccg tgggtgtttg
     1141 cttgggcaca gaaccggcac gtgattaccg ggtggtatgg cgtgggcagt gggctagcga
     1201 gcttcctcga ggttcgaggt gagcgcggaa gggagttgtt aggccgcatg ttcatgcagt
     1261 cgcgcctctt ccggctcatc gtcgatgagg tggagaagac ccttgccctg gtcgatcttt
     1321 acatagccgg ccagtatgcg gacctagtcg ccgataaggc gctgcgcggg gcaatcttct
     1381 caacgattga aagcgaatat gtgctgaccc gtgacgcgat tctacgcatc accggcagcg
     1441 acactattgc agagcgcttt agagaatatc gtgagcggct aaatagccga ttatccatca
     1501 ttaatcaggt caatcgggag caagttgaac tcctgcgtcg ctttcgcact gcgcaaaata
     1561 aacaagcgag gcaggaatta aagtcagccc tcctgctatc gatcaactgc atcgccgccg
     1621 gcctcggcgc cacaggctga actagagaaa ggaattatca tgagctttac tctcattgaa
     1681 caggcgcggc ctcggctaca tcgttcagaa ttggccgtcc ctggatcgaa cccttctctt
     1741 tttgaaaaat cggcaaaatc tag
//
LOCUS       DATLJT010000682         6040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4407212,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000682 DATLJT010000000
VERSION     DATLJT010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4407212"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VM910_15720"
     CDS             <1..207
                     /locus_tag="VM910_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64011.1"
                     /translation="RTSEMRVGVIRPGNSRTIARGCGSTITTLTSGSSDWLLAEATAE
                     GGSLQPTRGGATIASINKMPADGA"
     gene            800..1789
                     /locus_tag="VM910_15725"
     CDS             800..1789
                     /locus_tag="VM910_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HVI64012.1"
                     /translation="MPSGPSSRSAMEIELTKLSSPRIFLVRMLVFLVLCALVMVVLYR
                     QIIQAFFANPGLNALIGLVLLVGIILSFRQVIRLYPEVSWVNNFRVADPGLTVARHPT
                     LLAPMAAILGGERTGRMTISQQTMRHLLDSIATRLDEARDISRYMTGLLVFLGLLGTF
                     WGLIETVGSVGEVIHDLKVGGDAGALFDTLKQGLARPLGGMGISFSSSLFGLAGSLIL
                     GFLDLQSSQAQNRFYTDLEDWLATTVREYGSDAAPAGDVQASLERLRAAVEDGGGRTT
                     TTAMSNLAEAIQGLVAHMRTEQQMIREWADGQAEQNGEIKRLLERLARQHEKS"
     gene            1807..2835
                     /locus_tag="VM910_15730"
     CDS             1807..2835
                     /locus_tag="VM910_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HVI64013.1"
                     /translation="MALARTRRSESPFNYWPGFVDALSTLVLSIIFLLTVFLVVQFFL
                     SQEVTGKDKALEELNAKIAQLNDMLSLEKLGKISLDDQLAQLKAGLASAESERDRVKG
                     LYEGLASAGNDAAGRTAELNKALESEKSVTARALAQIEVLNQQISALRRQLAALEEAL
                     DASEKRDKESQSRIADLGQRLNVALAQRVQELSRYRSEFFGRLRAILGNRPDIRIVGD
                     RFVFQSEVFFDTGQATLLPEGRAELDKVAGALLDLDKQIPSEIAWVLRVDGHTDVRPI
                     NSPIFKSNWELSSARAISVVQYLISLGVPAQRLVAAGFAEFQPLDTGITEDAYRRNRR
                     IELKLTER"
     gene            complement(2858..4705)
                     /locus_tag="VM910_15735"
     CDS             complement(2858..4705)
                     /locus_tag="VM910_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HVI64014.1"
                     /translation="MSAVERLDQAGGSAPPPPGVLVTEAAAAESERGKGRARLRPLLA
                     LSPYVARYRGRAFLALIALTIAALTTLLVPIAVRRMIDFGFTPEGIALINSYFSVMIA
                     VVGVLALASASRYYLVMTIGERIVADLRRDVFAHLMSLSPAFFDSARSGELISRLTAD
                     TTQIKSAVGASVSIALRNLMLFFGAAAMMVVTSPRLSGFVLLAIPLIVLPLVAFGRWV
                     RRLSRNAQDTLADASAYASELVGGIRTVQAYTGERLANARFGGEVEEAYEAARVSTQA
                     RAVLTAIIIFIVFTSVVAILWVGSHDVLTHAITPGRLGQFVLYTAFAAAGLGQLSEVW
                     GEVSAASGAAERLFEILRVKPEITAPASPRALPSPPRGDVTFDSVSFSYPMRTEVPAI
                     HQVSFSVKAGEKVAIVGPSGAGKSTLFHLLLRFYDPSSGVISFDGVPIKSADPRELRS
                     RIALVPQESVVFAASARDNIRFGRPEADDADVERAAELAHATEFIRALPNGFEASLGE
                     RGVTLSGGQRQRIAIARAILRDAPLLLLDEATSSLDAESETLVQTALEELMRHRTTLV
                     IAHRLATVLSCDRILVLDGGRIVEQGTHAALVAANGLYARLARLQFEGA"
     gene            4883..5110
                     /gene="rpmE"
                     /locus_tag="VM910_15740"
     CDS             4883..5110
                     /gene="rpmE"
                     /locus_tag="VM910_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HVI64015.1"
                     /translation="MKAEIHPNYHTITVVMTDGTEFQTRSTWGKEGDKLHLDIDAKSH
                     PAWIGGAQQILDRGGRVSRFQKKFSGFLKKE"
     gene            complement(5215..5727)
                     /locus_tag="VM910_15745"
     CDS             complement(5215..5727)
                     /locus_tag="VM910_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HVI64016.1"
                     /translation="MTDRSLGDGALVQFNEWLTNSAAFGNLFREGMDLVEETAAYLDG
                     AGRAEAKALDRAASLTYATESMRLTTRLMQLASWLLLHRAVKEGEMTLTQANREKTKV
                     KLSAADPGPSDVLERLPQQLQDLIARSMNLQSRVRRLDVTMHAPSAERAPIGNPLVPQ
                     LNRLKAAFER"
BASE COUNT         1157 a         1908 c         1919 g         1056 t
ORIGIN      
        1 cgcacctcgg agatgcgcgt cggcgtgatc cggcccggca attcacgcac catcgcgcgc
       61 ggttgcggct cgacgatcac gacgctgaca tcgggctctt cggactggct gctggcggag
      121 gccaccgccg aaggcggctc attgcagccg acgagaggcg gcgccacaat agcgagcatc
      181 aacaaaatgc ctgccgacgg cgcatgaatt ccagacattg gaacaagtac cccggttttg
      241 tgctcggagg cggacatgaa atccgcgcgg cgcaacggaa ctgcgcctgg cagggggaac
      301 ataaaaatga cccgtggcgt cgcaatacgg ccctatgtca cagccgttat ggctctgaaa
      361 caacaggtct tttgagggga tcaccctatt gttatggcga attgcgcctc gtgaatgaat
      421 ggaacccgaa tcaacgcggg acccgcagcc ggtaatggct gacgctccat ttgctgcctg
      481 ctttcaggag ttcgcgcgcg acgcgtcgcc cagcgaatct cggaaaatga gggcgacgag
      541 cgtctcgatt gcgaaattcg catcattatg tgggtccctc gtttgcgaac ttcggaatcg
      601 agggacacta gcgacttgtt gatctagtgt ggctttggtt cagaagtctg actcgcggca
      661 ggaacgatgc ggacttctga accgccacac tggcagaatt accaccctgg tgccgttttt
      721 cgaattgaag tctttgagtc gagcttgccg cgagccgtgc agggacttca aatggcggca
      781 ctagctaacg gatatcagaa tgccttcagg cccctcttcc cgctccgcga tggagatcga
      841 actgactaaa ttgtcctcgc cgcggatttt cctggtgcgg atgctggtct tcctggtttt
      901 gtgcgcgctg gtcatggtgg tgctgtatcg gcagatcatc caggccttct tcgccaatcc
      961 gggcctcaat gctctgatcg ggctcgtgct gctggtcggc atcattctgt cgttccgcca
     1021 ggtgatcagg ctttatcccg aagtgagctg ggtcaacaat ttccgcgttg ccgatcccgg
     1081 gctcaccgtc gctcgtcatc cgacgctgct ggcacccatg gccgcgattc tcggcggcga
     1141 gcgcaccggg cggatgacaa tctcgcagca aacgatgcgg catttgctcg attccatcgc
     1201 gacccggctc gatgaggcgc gcgacatctc gcgctacatg accggccttt tggtgttcct
     1261 gggacttctc ggcactttct ggggcctgat cgagaccgtc ggttcggtcg gcgaagtgat
     1321 tcacgatctg aaggtcggcg gcgatgccgg cgcgttgttc gatacgctca agcaggggct
     1381 ggcgcgcccg ctcggcggca tgggcatctc cttttcgtcc tcgctgttcg gtcttgccgg
     1441 atcgctgatc ctcggcttcc tcgacctgca gtcgagccag gcgcagaacc gcttttatac
     1501 cgatctcgaa gactggctcg ccaccacggt tcgcgaatat ggcagcgacg ctgcgcccgc
     1561 cggcgacgtg caggcctcgc tcgaacggct gcgcgcggcg gtcgaggacg gcggcggccg
     1621 caccacgacc acggcaatgt ccaatctcgc cgaagcgatc caggggctgg tcgcgcatat
     1681 gcgcaccgag cagcagatga tccgggaatg ggccgacggc caggccgagc agaacggcga
     1741 gatcaagcgg ctgctcgagc gcctcgcgcg ccagcatgag aagagctgaa accctgggag
     1801 aagccgatgg ccctcgcccg cacccgccgc agcgaatccc cattcaacta ctggcccggc
     1861 ttcgtcgacg ccctgtcgac gctggtgctt tccatcatct tcctgctgac ggtgtttctg
     1921 gtcgtgcagt ttttcctgtc gcaggaggtc accggcaagg acaaggcgct cgaggaactc
     1981 aacgccaaaa tcgcgcaatt gaacgacatg ctgtcgctgg aaaagcttgg caagatcagc
     2041 ctcgacgacc agctggcgca gctcaaggcg ggccttgcct cggccgaatc cgagcgcgac
     2101 cgggtcaagg gcctttacga agggctcgcc agcgccggca atgacgcggc cggccgtacc
     2161 gccgagctca acaaggcgct ggaatcggag aagtcggtca cggcccgtgc gctggcgcag
     2221 atcgaggtcc tgaaccagca gatcagcgcg ctccgccgcc agctcgcggc actcgaggaa
     2281 gcgctcgacg cctcagagaa acgcgacaag gaatcgcaaa gccggatcgc cgatctcggc
     2341 cagcgcctca acgtggcatt ggcccagcgc gtgcaggaat tgtcgcgtta ccggtcggaa
     2401 ttcttcggcc gtctgcgcgc cattctcggc aaccggcctg atatccgcat cgtcggcgac
     2461 cgtttcgtgt tccagtcgga agtgtttttc gacaccggtc aggccacgct gttgcccgag
     2521 gggcgcgccg aactcgacaa ggtcgcaggc gcactgcttg acctggacaa gcagattccg
     2581 agcgaaatcg cctgggtgct gcgggtcgac ggccataccg acgtccggcc gatcaacagc
     2641 ccgatcttca agtcgaactg ggaattgtcg tcggcgcgcg ccatttcggt ggtgcaatat
     2701 ctgatctcgc tcggcgtgcc cgcgcagcgg ttggtcgccg ccggcttcgc cgagtttcag
     2761 ccgctcgaca ccggcattac cgaagacgcc taccggcgca accgccgcat cgagctgaag
     2821 ctgaccgaac ggtagtgcct tttcgcgcga cgacgaatta agcgccttcg aactgcaggc
     2881 gcgcgaggcg ggcatagagc ccgttggcgg cgaccagcgc ggcatgggtg ccctgctcga
     2941 cgatgcggcc cccgtccagc accaggatac ggtcgcagga caggaccgtg gcaagccggt
     3001 gcgcgatcac cagcgtggtg cgatggcgca tcaattcttc caacgcggtc tgcaccagcg
     3061 tctcgctttc ggcgtcgagc gaggaggtcg cctcgtccag cagcaatagc ggggcgtcgc
     3121 gcaggatggc gcgtgcgatc gcgatgcgct ggcgctggcc gcccgacagc gtcacgccgc
     3181 gttcgccgag cgaggcctcg aatccattgg gcagcgcgcg gatgaactcg gtggcgtgcg
     3241 cgagttcggc tgcccgttcg acgtcggcgt cgtcggcctc gggccggcca aatcggatgt
     3301 tgtcacgtgc gcttgcagcg aacaccaccg attcctgcgg caccagcgcg atgcggctgc
     3361 gcaactcgcg cggatcggcc gatttgatcg gcacgccgtc gaaggagatc acccccgagg
     3421 acggatcgta gaaccgcagc aaaaggtgga acagcgtgct ctttcctgca cccgaagggc
     3481 cgacgattgc gaccttctcc ccggccttga ccgagaacga cacctgatgg atcgccggca
     3541 cctcggtgcg catcggatag gaaaagctga cactgtcgaa cgtcacgtca ccgcgcggcg
     3601 ggctcggtaa cgcgcgcgga ctggcgggtg cggtgatctc cggcttgacg cgcaggatct
     3661 cgaacaaccg ctcggcggcg ccggaagctg ccgaaacctc gccccagact tcgctgagct
     3721 ggccgaggcc ggccgcggca aaggccgtat agagcacgaa ctggccaagc cgtcccggcg
     3781 tgatcgcatg cgtcaggacg tcatgcgatc cgacccagag aattgcgacc acgctggtga
     3841 agacgatgaa gatgatgatc gcggtcaaca ccgcgcgcgc ctgggtcgag acccgcgcag
     3901 cctcataggc ctcttccact tcgccgccga agcgcgcatt ggcgaggcgt tcgccggtat
     3961 aggcctgcac ggtcctgatt ccgccgacca gctcggaggc gtaggccgag gcatccgcca
     4021 gggtatcctg agcgttgcgc gacagccgcc gcacccagcg cccgaacgcc accagcggaa
     4081 gcacgatcag cgggattgcc agcagcacga agcccgagag gcgcgggctc gtcaccacca
     4141 tcatggcggc ggcgccaaaa aacagcatga ggttgcgcag cgcgatcgac accgacgcgc
     4201 cgaccgccga cttgatctgt gtggtatcgg cggtgagccg cgagatcaat tcgccgcttc
     4261 ttgcggaatc gaagaacgcg ggcgacagag acatcagatg ggcgaacacg tcgcggcgga
     4321 gatcggcgac gatgcgctca ccgatggtca tcacgaggta gtaacgcgag gcgctggcga
     4381 gcgcgagcac ccccaccacc gcgatcatga cggagaagta gctgttgatc agcgcgatgc
     4441 cctccggcgt gaagccgaag tcgatcatcc gacgcaccgc gatcggcacc aggagcgtgg
     4501 tcagcgcagc gatcgtcaac gcgatcaagg caaggaaagc gcggccgcgg tagcgtgcga
     4561 cgtaaggcga aagcgcaagc aatggccgca gccgcgcccg gccctttccg cgctcgctct
     4621 cggccgcagc cgcctcggtg acgaggacgc ccggcggagg cggcgcgctg ccaccagcct
     4681 gatccagccg ttccactgcg ctcattagat ccgacccgct ctttcgatat gcaccagata
     4741 ggacgggctc caggccgagg caaacccgga aaaatcctta tggaatcgcg gcttgttttg
     4801 ccgggggcca tgcgttatag agccgcccaa atccgtcatt gctatgtttg aaagacaggc
     4861 gcggaccgcg cccgaggaag ccatgaaagc cgagattcac ccgaactatc atacaattac
     4921 ggtcgtgatg accgacggca ccgagttcca gacccgctcc acctggggca aggaaggcga
     4981 caagctgcac ctcgacatcg acgcgaaatc gcacccggcc tggatcggcg gcgcccagca
     5041 gatcctcgat cgtggcggcc gcgtgtcgcg ctttcagaag aaattttcgg gcttcctcaa
     5101 gaaggaatag agttccgcca ggcggacggc aaaacgcccc cgaacggggg cgtttttgtt
     5161 tgtgccgtca tgctcacgct cctcgctgcg gcgcggctga gatgtctcta acctttaccg
     5221 ctcgaacgca gccttcagcc ggttgagctg cggcaccagc ggattgccga tcggcgcgcg
     5281 ttcggcgctc ggtgcatgca tcgtgacgtc gagccggcgc acccgcgact gcaggttcat
     5341 cgagcgcgcg atcaggtcct gcaattgctg cggcagccgc tcgagcacat cggaagggcc
     5401 gggatcggct gcggacagtt tgaccttggt tttctcgcgg ttggcctggg tcagcgtcat
     5461 ctcaccttcc ttcaccgcgc ggtgcaagag aagccaggag gcgagttgca tcaggcgggt
     5521 ggtcaaccgc atgctttcgg ttgcataggt gaggctcgct gcgcggtcga gcgccttggc
     5581 ctcggcgcgg ccggcgccgt cgagataggc agcggtctcc tccaccaagt ccatgccttc
     5641 acgaaacaga ttgccgaacg ccgccgaatt ggtgagccac tcgttgaact gaaccagagc
     5701 gccatcgcca agcgaacgat cggtcatggt taacgccttt tcacgccaca attacgctgc
     5761 cggcctcgcg cgccggctta tgatgaacaa atcattgcgc gtgaggatcg aagagtccag
     5821 cgacaagacc gtttatggtt tccaagcggt ggggccgttg caagacggcg cggttgcagc
     5881 gtccacgcga acatcgaaga gccgcgcgcg caaaaaaaga gccgccgttt ccggcggctt
     5941 ttgaagttga taacagggag gcgtcaaaca gagtggacag gagccactcg gtgtccaaac
     6001 gaggacaatg acagtcataa aggcgaaagc ttaatcgatc
//
LOCUS       DATLJT010000683         4301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_730170,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000683 DATLJT010000000
VERSION     DATLJT010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730170"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="VM910_15750"
     CDS             complement(<1..1791)
                     /locus_tag="VM910_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HVI64017.1"
                     /translation="MTGDHRDRIAQHNSKDVPRFAPNFTVYVLPSKVVCLYSEDRKFF
                     LHGELYCALAAAIGEGGKSFRELVRVLEGNFPSDKIHEALKRLVDRRFIIAKPRASAD
                     TVAAYWASLGLSPEVAETNLQKCRVRIQSVDVHGATELATALKALGARVVKRSAELTV
                     TLVSDYLEGQLAELNQEHLSDRTPWILAQPCGIFPLVGPVLSPGKGACWACLAERMRR
                     NREIKALLDRGLARRLVTSPLARDMVGHSGIQLAAVEIAKAIATDFRTDLRKHIISLD
                     LLGATIVKHYVAARPQCPACGRKKLRDPRRAPVPVELGAGGKLVMTSGGYRTVSSRAT
                     VAQFRKHVSPLTGVVSRLERIEADLPMNTNYLATHNFSGPARTVNELKAGLSGGSFGK
                     GSTAEQGEASALMEAIERYSGIFQGDEIRVTRRFTDFPSGDAILPNDVLLFSDAQYQR
                     DQSQAIDWDATPMPFPFDRSAEIEWSPVWSLRDERFKYLPTSLLYFFYRGPPTAYHVH
                     ADSNGCAAGNTLEEAIVQGFLELVERDAYAIWWYNRLQRAEVDLGQFDEAYIRDLKIQ
                     LAETGRRLWMLDVTSDLGIPSFVTIAHWMEN"
     gene            2788..3612
                     /locus_tag="VM910_15755"
     CDS             2788..3612
                     /locus_tag="VM910_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64018.1"
                     /translation="MANSHGRFVWYELMTTDMEAAKAFYAEVVGWGTQDASMPGMAYT
                     LFTAGGTSISGLMGLSEDARKSGLRPSWLGYVGVDDVDATADRIKELGGAVHVPPTDI
                     PNISRVSVAVDPQMATIALFKWLKGGQEQPPALDAPGGVGWHELIAADWEKAWPFYRE
                     LFGWQKAQADTSAMGPYQLFSAGGETIGGMFTKPATEPVPFWLYYFNVGDIDVAMRRV
                     RAGRGEIVHGPIEVRGGRWMAQCTDPQGAIFALVGKRSHNGIGYFEPVASRNRRVG"
     gene            complement(3696..4025)
                     /locus_tag="VM910_15760"
     CDS             complement(3696..4025)
                     /locus_tag="VM910_15760"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI64019.1"
                     /translation="MMVVVFRARRTPEGDGQEYRQWFLRMSELARKMPGYISHKGYAA
                     EDGERLTLFEWKSADTLRAWATHPEHLPVQELGRQKFYTQYHLQVCELVRESKFVRDA
                     KRARQAV"
BASE COUNT          829 a         1349 c         1303 g          820 t
ORIGIN      
        1 gttttccatc cagtgcgcta tggtcacaaa gctcggaatc ccgagatcgc tggtgacgtc
       61 gagcatccag aggcggcgtc cggtttcggc aagctggatt ttcagatcac ggatgtaagc
      121 ttcatcgaac tggccgagat cgacttccgc ccgttgcaat cgattgtacc accagatcgc
      181 gtatgcatcc cgctccacca actcgaggaa cccttggacg atcgcctcct cgagcgtgtt
      241 gccggccgca cagccgttgg aatccgcatg cacgtggtag gcggtaggcg ggccccggta
      301 aaaaaaatat aacaggctgg tcggaagata tttgaaccgt tcgtcgcgca gcgaccagac
      361 cggcgaccat tcgatctcgg cggatcgatc gaaggggaac ggcatcggcg tcgcgtccca
      421 gtcgatcgcc tgcgactgat ctcgctggta ctgcgcgtcg ctgaacagca agacgtcgtt
      481 cggtagaatg gcatctcccg atgggaaatc cgtgaagcgc cgggttactc tgatctcgtc
      541 gccctgaaaa atcccggaat agcgctcgat cgcctccatg agtgcgctgg cttcaccctg
      601 ctctgccgtg ctgcccttgc cgaagctgcc gccgcttagt cctgccttga gctcattgac
      661 cgtcctcgcc ggccctgaaa aattatgcgt agcgagatag ttggtgttca tgggcaaatc
      721 ggcttcaatc cgctcgagcc gcgagaccac gccggtaagg gggctcacgt gtttgcggaa
      781 ttgggccacc gtcgcccgcg acgaaacggt ccggtagccc ccgctcgtca tgaccagctt
      841 gccgccggcg ccgagttcga ccggcaccgg tgcgcgacgg gggtcgcgca acttcttgcg
      901 gccgcaggcc gggcattgcg gacgggccgc tacgtagtgt ttcacgatgg tcgcgcccag
      961 caggtcgagg ctgatgatgt gcttgcgcag gtcggtacgg aagtcggtgg caattgcttt
     1021 tgcgatctcg acggccgcaa gctggatgcc gctgtggccg accatgtccc gggcaagcgg
     1081 cgaagtgacg aggcggcgcg ccagcccgcg atcgagaagc gccttgatct cccgattgcg
     1141 tctcatgcgt tcggcaagac aagcccagca ggcgcccttg cccgggctga gcacgggtcc
     1201 caccaggggg aaaatgccgc acggttgggc gagaatccaa ggtgtgcggt ccgataagtg
     1261 ctcctggttc aattcggcta gctgtccttc gagataatcg cttaccagcg tgaccgtcag
     1321 ctcggcggag cgcttcacga cgcgagcgcc tagtgccttc aaggccgtag cgagctcggt
     1381 ggcaccgtgt acgtcgaccg attgaatgcg cacgcgacat ttctggagat ttgtctccgc
     1441 gacctccggc gacaggccca ggctcgccca ataggcggcc acggtgtccg ccgaagcgcg
     1501 cggtttcgcg atgataaagc ggcgatcgac cagccgcttc agcgcttcgt ggattttgtc
     1561 cgacggaaaa ttcccttcca gcactcgaac gagctcgcga aaactctttc caccctcgcc
     1621 gatcgcggca gcgagcgcgc aatagagctc gccgtgaaga aagaacttgc ggtcctcgga
     1681 atagaggcac acgaccttcg acggcagcac atagacggtg aaattcggcg cgaaccgcgg
     1741 aacatctttg ctgttatgct gcgcgatgcg atcgcggtga tcgcctgtca tgcggtgagc
     1801 acgcgcggtt ctacgcttat cgcgaccggt ccggatcgcc attgcaagac atgtattctc
     1861 gagcttgcga gagcgccgtc gtctcgctcg tgcgccggcg tttctcgcta ggattgacca
     1921 tccactcggc cgtcatgcgg cgccgctaac aacacataat tggccgggtc gaacctggtc
     1981 aacccggcca tgatcttgcg tctgtcaacc taatcgggcg cgccgagact agcagacaag
     2041 tctagcagat acggcaaact ccccacgaca agcagcagcc gcagcctccg caacctccgc
     2101 agccgcagcc tccgcagcgg cagccgcagc ccctgccgca tctgcagccc ccgcagcgtc
     2161 cgcagccgcc gcagcgtccg caacctcggg caaatttttc gccaagtcgc ggcgttccgg
     2221 cgttttcctt gtcgaagacg tagaacgtcc ccaagctgac gtcggcgact tcttcctcac
     2281 tgagagtgat ttggggcggg gaggtatccc acgacggtac atctgcggcc ggaacagcgg
     2341 ttgccgcgga tgcgccgccc gccagcgaca aggacacgcc gacgactccc accaccggta
     2401 cgacttcctt cggatgtttt cgcttcgaag cacgcttcgt gtgcggcata gtcgagtcct
     2461 cccacgcctg ctagcgaatg ttcgacgccc gaaacactat tggtacgctt tggctaccga
     2521 cctggcaaga ccaattacgc atgctgatcg gcgtagctta cgcgaggctg acgcacactg
     2581 gcgcaggcaa cgtgaacaac cgttcataag acactcgatt cataagacac tcgaatcgcg
     2641 gccggcgtgc gctcacaaaa cgcgcggagg cgcgtcgagt tccgcgactt tgtcaatgac
     2701 atcgtcgccg cctcgattct gaagtagcct aggtcgaccg agtgcggatg ccgcgacgtt
     2761 ttgcaggctc gaaggagaga cacagacatg gccaattcgc atggccgttt cgtctggtac
     2821 gaattgatga cgacggacat ggaggccgcc aaggccttct acgccgaggt cgtcggctgg
     2881 ggcacacaag acgcgtcgat gcccggcatg gcctatacct tgttcaccgc tggagggacc
     2941 tcgatcagcg ggctgatggg cttgtcggag gacgcgagga aatcaggctt gcggccgagc
     3001 tggctcggat atgtgggcgt cgacgacgtg gacgccaccg ccgatcgcat caaagagctt
     3061 ggcggcgccg tgcatgtccc accgacggac atccccaaca tcagccgcgt ttcggtcgcg
     3121 gtcgacccgc aaatggcaac gattgcgttg ttcaagtggc tcaaaggcgg ccaagagcag
     3181 cctcccgcgc tggacgcgcc cggcggcgtc ggctggcatg agctgatcgc cgccgactgg
     3241 gagaaagcgt ggccgttcta ccgcgagctc ttcggctggc aaaaagcgca agccgacacc
     3301 agcgcgatgg gcccatatca gctgttctcc gccggaggag agaccatcgg cggcatgttc
     3361 acaaagcctg cgacggagcc ggtccctttc tggctttatt acttcaacgt cggcgacatc
     3421 gacgtggcga tgaggcgcgt gagggctggg cgtggcgaga tcgtccatgg cccgattgaa
     3481 gtgcggggcg gcagatggat ggcccaatgc accgaccctc agggcgccat tttcgctctg
     3541 gtgggaaagc gcagccacaa cggcatcgga tatttcgagc ccgtcgcatc acgcaatcgg
     3601 cgggtcggtt aaacgggaat tgtcggtcct accacgcggc gaatttcaat ctgcacaccg
     3661 tgccatccga attcccgtag cgcctgtgga agtgtctaaa ccgcttggcg agcccgtttg
     3721 gcatcgcgca cgaatttcga ttcgcgcacc aattcgcaaa cctgaagatg atattgagta
     3781 tagaatttct gccggcccag ctcctggacc gggagatgtt ccggatgggt ggcccacgcg
     3841 cgcagcgtgt ccgccgactt ccattcaaac agcgtgagcc gctccccgtc ttccgccgca
     3901 taacccttgt gcgatatgta accgggcatc ttgcgggcga gttcgctcat gcgcaggaac
     3961 cattgcctat attcctgacc atcgccctca ggcgtgcgcc gagcgcgaaa cacgaccacc
     4021 atcatcatcc gcctcctgtt ccaccgcctt tcagggccgc cggttggcgg cgcatgatcc
     4081 tttcccagcg tcagccatcg tgcgcagggt catcgggcga ggtatctttg agtccaccat
     4141 agcggccgat cgtacactat agaggccaaa ttgtctacta tcgcggacaa tgtgacggca
     4201 ggcgttgccc aggccatcaa gagccggcgc cagcagctga gactcacgtt gcgtgagctg
     4261 gcgacgagga gcgggatctc ctcctcgatg atttcggaca t
//
LOCUS       DATLJT010000684         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2339294,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000684 DATLJT010000000
VERSION     DATLJT010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339294"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VM910_15765"
     CDS             <1..765
                     /locus_tag="VM910_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64020.1"
                     /translation="FVGMVAAGISGYVIGRLSFKVRGAYFVIVTISFAEVTRLVALNW
                     VELTQGPLALTNIPPYTLGLPGLGDYTLWNKTAYYYLVVAVGLAAYFVIARLVGSRVG
                     RAMIALKENETLASSVGIDVTRYLVLAAVVSAAMAGGAGSLYAHYLKIIDPDIFLFIY
                     TVTMVIMVITGGKGTLAGPIVGGIIFGFLPVILRPFAAPAVQWILYGGLMIAIVFVLP
                     QGIVPALERSIANWAGSTRKPASAMAAGNSESRGGQ"
     gene            765..1559
                     /locus_tag="VM910_15770"
     CDS             765..1559
                     /locus_tag="VM910_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64021.1"
                     /translation="MPARVASRDVILSIEHVAVHFGGLVALSDLNFTVCNGEIVGLIG
                     PNGAGKTTAFNVFTGFLKPTAGEVRYRNTLLNGLKPHEIAGLGLVRTFQRTSIFQNNT
                     VFENVLIGLHRRGRSSLWDTLLALPREKVAEGELADEARELLRVVGLERRAAEIAGTL
                     PYGEQRLLGIALALAAQPSMLLLDEPVSGMNPSESAKVMTLVEAIRASGVTILLVEHD
                     MPMVMRVSDRIVVLNYGRIIAEGPPAAIQKNPDVIRAYLGEGKKRA"
     gene            1552..>2031
                     /locus_tag="VM910_15775"
     CDS             1552..>2031
                     /locus_tag="VM910_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64022.1"
                     /translation="MLELRDVVCCYGQVTALKGVSLTVGKGQLVALIGANGAGKTTTL
                     KAISGLLHPSAGTIVFDGHDITHPSARAILALGLAHCPEGRHVFPQMTVLENLEMGCY
                     LRADRRGIATDMTRVFDQFPRLAERRRQAAGTLSGGEQQMLAIGRALMSQPKLIMFDE
                     "
BASE COUNT          348 a          632 c          646 g          405 t
ORIGIN      
        1 ttcgtcggca tggtcgccgc cgggatcagc ggctacgtga tcggccggct gtcgttcaag
       61 gtgcgcggcg cctacttcgt gatcgtcacg atcagctttg ccgaggtgac gcggctggtc
      121 gcgctgaatt gggtggagct gacccagggt ccattggcgc tcaccaacat ccctccatac
      181 acgctcggac ttccaggcct cggcgactac acgctctgga acaagaccgc ctactattac
      241 ctcgtcgtcg ccgtggggct ggcggcctat ttcgtgatcg ctcggctggt cggctcccgc
      301 gtcggccgcg ccatgatcgc gctcaaggaa aacgagacgc tcgcaagctc ggtcggcatc
      361 gatgtgacgc gctatctcgt gctggcggcg gttgtgtccg ccgcgatggc gggcggcgct
      421 ggcagcctct acgcgcacta tctcaagatc atcgatcccg atattttcct gttcatctac
      481 accgtcacga tggtgatcat ggtgatcacc gggggcaaag gaaccctggc cgggccgatc
      541 gtcggcggaa tcatttttgg ctttctaccg gtgatcctgc gccccttcgc tgcgcctgcc
      601 gtccaatgga tcctttacgg cggcttgatg atcgcgatcg tcttcgtcct gccgcaaggg
      661 atcgttcccg cgcttgaacg ctccatagcg aactgggccg ggtccacgcg caagcccgcg
      721 agcgcaatgg cggccggcaa ctccgagtcg cgcggtggcc aatgatgccg gcgcgcgttg
      781 catcgcggga cgtgatcctt tcgatcgagc atgtcgcggt gcatttcggc gggctcgtcg
      841 ccctttccga tctcaatttc acggtttgca atggagaaat cgtcggcctc attggcccga
      901 acggtgccgg caagaccacc gctttcaacg tgttcactgg atttctcaag cccacggctg
      961 gcgaagttcg ctaccgcaac acgttgttga acggcctcaa gccacacgaa atcgcaggtc
     1021 ttggcctggt caggacgttc cagcgcacga gcatattcca gaacaacacc gtattcgaaa
     1081 acgtgctgat cggattgcac cgccggggac ggtccagcct gtgggacacg ttgctggcgt
     1141 tgccgcgcga gaaggtcgcg gaaggcgagc ttgccgacga agcgcgtgag ctcctgcgtg
     1201 tggtcggact cgaacgccgc gcggcggaaa ttgccggcac gcttccttac ggcgagcagc
     1261 gcctgctcgg catagctttg gcgcttgccg cgcagccctc gatgttgctg ctggacgagc
     1321 cggtctccgg catgaacccg agcgagagcg cgaaggtgat gacgttggtc gaggccattc
     1381 gcgcctcggg cgtgaccatc cttttggtgg agcacgacat gccgatggtc atgcgggtgt
     1441 cggaccgcat cgtcgtcctc aactacggcc ggatcatcgc agaaggcccg ccggcggcta
     1501 tccagaaaaa tcccgacgtc attcgcgcct acttgggaga aggaaagaag cgtgcttgag
     1561 ctgcgcgatg tcgtctgttg ctacggccag gtgacggcgc tcaagggcgt gtcgctcacc
     1621 gtcggcaaag gccaactcgt cgccttgatc ggggcgaacg gagccggcaa gaccacgacc
     1681 ttgaaggcga tctcgggatt gttgcatccg agtgccggca cgatcgtgtt cgatggacac
     1741 gacatcaccc acccctccgc acgcgccata cttgcgctcg ggctcgcgca ttgtccggag
     1801 ggacgccatg tgttcccgca gatgacggtg ctggaaaatc tggaaatggg ctgctatttg
     1861 cgcgcggatc ggcgagggat cgcgaccgat atgacgcgcg tcttcgatca gtttccgcgg
     1921 cttgccgagc gccgccggca ggcggcggga accttgtccg gcggcgagca gcagatgctt
     1981 gcaatcggcc gagccttgat gtcgcaaccc aagctcatca tgttcgacga g
//
LOCUS       DATLJT010000685         1608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_272391,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000685 DATLJT010000000
VERSION     DATLJT010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272391"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VM910_15780"
     CDS             complement(<1..840)
                     /locus_tag="VM910_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64023.1"
                     /translation="MAAVDESADIAISAPATTAEAESSRSSRPDAGEERSEWMAGLTM
                     SVARWVVRLSALLAGLVCWHYASSMGLTLYVRFEQIPGPLKVGEALINQLQHVAFYLH
                     IGASLRRIAISYSLAAGIGITLGLLMGRSRFIRDLIFPYVEILRPIPGVAWIPLAILL
                     WPTEEGSIIYITFLGALFPVILNTLHGVEQTPEVLVRAAQSLGASRMCIFRHVVLPGA
                     LPSICTGLAIAMGVSWFSLLAGEIISGQYGIGYFTWNAYSIINPPDIIVGMLTIGLLG
                     AATT"
     gene            complement(910..>1608)
                     /locus_tag="VM910_15785"
     CDS             complement(910..>1608)
                     /locus_tag="VM910_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI64024.1"
                     /translation="GIPYLHGTVVRQDFAEKYPEVVVAFVKAVYDAGKWIDEDPMRAT
                     DLMEKWTGVEKEVLYLYFSKGGHLTQDPTLKKQWIDTLKVDHQVLVNDNLAPALDFDA
                     WIKEDYLKRAYAEMGLDYEAEKAKVVDTLVTNAGRPNEIWHARDGIVSYPTLKEFLKA
                     VAEFRASGQKLNATYVYDQATGLKLFGKTAFFVHKRDGDFATFLRKGEAEEFAAKVGG
                     KVISFEEAVASSAS"
BASE COUNT          313 a          488 c          503 g          304 t
ORIGIN      
        1 cgtggtcgcg gcgcccaaca gcccaatggt cagcataccg acaatgatgt ccgggggatt
       61 gatgatggaa taggcgttcc aggtgaaata gccgatgcca tattgcccgc tgatgatttc
      121 gcctgccagc aacgagaacc aggacacgcc catggcgatc gcgaggcctg tgcagatgct
      181 cggtagcgct cccggcagga cgacgtggcg gaagatgcac atgcgcgagg caccgagcga
      241 ctgcgccgcg cgaacgagca cttccggcgt ctgctccacg ccgtgaaggg tgttgagaat
      301 gacggggaac aacgcgccga ggaaggtgat gtagatgatc gagccttcct cggtcggcca
      361 gagcaggatg gccagcggaa tccacgcgac ccccgggatc ggacgcagaa tctcgacata
      421 ggggaagatg aggtcgcgga tgaaccgcga gcgccccatc agcagaccga gcgtgatccc
      481 gatgccggcc gccagcgaat aactgatggc gatgcgccgc aggctggcgc cgatgtgcaa
      541 gtagaacgca acgtgctgca gttggttgat cagcgcttcg ccgaccttga gcggaccggg
      601 gatttgctcg aaccgaacgt ataaggtgag ccccatggag ctcgcgtagt gccagcaaac
      661 aagcccggcc aacagcgccg acagccgcac cacccagcgg gcaaccgaca tcgtaaggcc
      721 ggccatccat tctgatcgct cctcgcccgc atcgggccgc gacgaacgcg aactttcagc
      781 ctcggcggtc gtcgccggcg cgcttatcgc gatgtcggcg ctctcatcga cggccgccat
      841 tttcactgtc gcttgcggag caatgcgtgc gtgcgcgttc acgagtgaga gcgccttgct
      901 ttgggattgt catgacgccg agcttgcaac agcttcctca aacgagatga ccttgccgcc
      961 gacttttgcg gcgaactcct cggcctcacc cttgcgcagg aaggtcgcaa agtcgccgtc
     1021 gcgcttgtgg acgaagaacg ccgtcttgcc gaagagcttg agtcccgttg cctggtcgta
     1081 gacataggtc gcattgagct tctggccgct ggcgcggaat tctgcgaccg ccttgaggaa
     1141 ctccttcagc gtcggatagg agacgatgcc gtcgcgcgcg tgccagatct cgttcgggcg
     1201 gccggcattc gtcacgagcg tatcgacgac cttggccttt tcggcttcgt aatctagtcc
     1261 catctcggcg taggcgcgct tgagatagtc ctccttgatc caggcgtcga agtccaaggc
     1321 gggggcaagg ttgtcgttta caaggacctg atgatcgacc ttgagcgtat cgatccactg
     1381 cttcttgagc gtcgggtcct gcgtgagatg gccgcccttg gagaaataga ggtagagcac
     1441 ttctttctcg acgccggtcc acttttccat cagatcggtc gcccgcatgg gatcctcatc
     1501 gatccatttg ccggcgtcgt agaccgcctt gacgaaggcg acgaccacct ccggatattt
     1561 ctcggcgaag tcctgtcgca cgacggtgcc gtgcagatag ggaatccc
//
LOCUS       DATLJT010000686         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4407425,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000686 DATLJT010000000
VERSION     DATLJT010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4407425"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VM910_15790"
     CDS             complement(<1..591)
                     /locus_tag="VM910_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64025.1"
                     /translation="MAAEQGDFRGPSTTHPPPENHQLEKGRPFAMQNVLITGGAGYVG
                     HLLTPRLLSEGYHVTVYDQLYFGCRLPNSPDLKVIQGDIRDTPKLAEALVGQDAVLHL
                     ACISNDASFELDEKLSESINYDCFEPLVVAAKKAGVKRFVYCSSSSVYGVSDSPDVTE
                     DHPLVPLTLYNKFKGMCEPVLWKHKADDFTCVVIRPA"
     gene            complement(745..>2162)
                     /locus_tag="VM910_15795"
     CDS             complement(745..>2162)
                     /locus_tag="VM910_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64026.1"
                     /translation="RVDAESGVLAVKPREMAKLALTHKHLTVKARTVNQRLYHQWCMD
                     QFYWYVDEETLVGYQWPVALVAIKPERVITQPVLVWDFGYLPEAAPTLEPCFLADSDE
                     FFMIEPQKRVTGEELVRPGGISLDAIAKFLSDWTTREQRRCGRQLLTIHAGELPLDID
                     DVIAESRRYMNEIERRLSAEPQSHIGHGQLGRWFTETTRRLEARSAGGSSPAVSTSPA
                     LEKEARGLSHILRSVHTALFGPLPFLRPAHPLWLDTFDVTAQLVSWSRAGQRILWLCS
                     RDSLFSSILKDRRDPTSLLMTTPASQAARGDFFDACLCELSPSELMMLPALHRRIRIL
                     VRNGGKVVVHINNPYLAPVGMGHFAAYETVFPDVDRSSIRFYGTWLTAQIRKLYLRAA
                     TSFPAGSLARGVLAGLALVALAPMAWLANRAAANRDASAFTLNWTSVVVELEVARKEM
                     SASPCAKRGASGRVHAERDTT"
BASE COUNT          407 a          669 c          701 g          385 t
ORIGIN      
        1 ggcgggacgg atgacgacgc aggtgaagtc gtccgccttg tgcttccaca gcaccggctc
       61 gcacatgcct ttgaacttgt tgtagagcgt gagcggcacg agcggatggt cttcggtcac
      121 gtcgggcgag tcgctcacgc cgtagaccga gctcgaggag cagtacacga accgtttcac
      181 gcccgccttc ttggccgcga ccaccagggg ctcgaaacaa tcgtagttga tagactccga
      241 aagtttttca tcgagctcga agctcgcgtc gttggagatg caggcgagat gaagaacggc
      301 gtcctgcccg acgagcgcct cggcgagctt gggcgtgtcg cggatatcgc cctggatgac
      361 tttcagatcg ggagaattgg gcagccggca accgaaataa agctggtcat agaccgtaac
      421 gtggtagcct tctgaaagca ggcgcggcgt gaggagatgg ccgacatagc cggcaccacc
      481 ggtgatcagc acattctgca tagcgaacgg acgccctttc tcgagctggt gattttcggg
      541 agggggatgc gtggtggacg ggccgcgaaa gtccccctgc tcggcagcca atttcgggag
      601 cctcatacac attttttgcg gtcacgtctc ctgtttttgc gaggtcgcaa cgcgcccatg
      661 gagcggcccc cggcgccctg accgcgtgcc ggcgaacggg aagattctgt cgttggatca
      721 gcttgttagc tcgctcgaac gccgctaggt cgtgtcccgt tcggcatgca cgcgcccgct
      781 cgcgccgcgc ttggcgcaag gggatgcgga catctccttg cgagcgacct cgagttccac
      841 gaccacgctc gtccagttca gcgtgaatgc gctcgcatcg cgattggccg cagccctgtt
      901 cgcgagccaa gccatcggcg ccaatgcgac caatgccagt cccgccaaga cgccccgggc
      961 cagcgacccc gccggaaacg aggtggcagc gcgaaggtag agcttgcgaa tctgcgccgt
     1021 gagccacgtg ccgtagaacc gaatgctcga cctgtcgaca tccggaaaca cggtctcgta
     1081 ggccgcgaag tgccccatcc cgaccggcgc cagataggga ttgttgatat ggacaacgac
     1141 cttgccgccg ttcctcacca agatgcggat gcggcgatgc aaggcgggga gcatcatcag
     1201 ctcggatggg ctgagctcgc acaagcaggc atcgaagaaa tctccgcgcg ccgcctgact
     1261 cgccggcgtc gtcatcaaca ggctcgtcgg atccctgcgg tctttcagga tgctgctgaa
     1321 caacgaatcg cgggagcaca gccagagaat ccgctgaccc gctcggctcc aggacacgag
     1381 ttgcgcggtg acatcgaagg tatcgagcca cagcggatgc gcaggtcgca ggaacggcaa
     1441 cggaccgaac agcgcggtgt ggaccgagcg gagaatatgc gacaacccgc gggcttcctt
     1501 ttcgagcgcc ggtgacgtcg acacggccgg gctgctgccg ccggcacttc tcgcttcgag
     1561 gcgacgggtc gtctccgtga accagcgccc gagctggccg tgtccgatat gcgactgggg
     1621 ctcggccgac agtcggcgtt cgatttcgtt catgtagcga cgcgactccg cgatcacgtc
     1681 gtcgatgtcg aggggaagct cgccggcgtg aatggtcagg agctgtcggc cgcagcggcg
     1741 ctgctcgcgg gtggtccagt cggagagaaa cttggcgatg gcgtcgagcg aaatcccgcc
     1801 cggacgcacg agttcttcgc cggtcacgcg cttctgcggc tcgatcatga agaactcgtc
     1861 ggaatcggcg aggaagcaag gctcgagcgt cggcgccgcc tccggaaggt agccgaagtc
     1921 ccataccagg accggctgcg tgatcacccg ctccggcttg attgcgacga gagccaccgg
     1981 ccattggtag cccaccagtg tttcctcatc gacgtaccaa tagaactgat ccatgcacca
     2041 ttgatgatag aggcgctgat tcacggtgcg cgccttcacg gtgaggtgct tgtgggtcaa
     2101 ggcgagcttt gccatctcgc gcggcttgac cgcgagcacg ccgctctctg catcgacccg
     2161 cg
//
LOCUS       DATLJT010000687         7550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1880280,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000687 DATLJT010000000
VERSION     DATLJT010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880280"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VM910_15800"
     CDS             <1..401
                     /locus_tag="VM910_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64027.1"
                     /translation="SPLDVRDAGEIERVIALFGRSSDGGLIVTGSALAAAHRKLIVAL
                     AAPHRLPAVYPFRYFATSGGLISYGPDTTDPFRRAAGYVARILNGEKPGDLPVQAPTK
                     YELVINLKTAKALGLEVPPTLLARADEVIE"
     gene            complement(515..1549)
                     /locus_tag="VM910_15805"
     CDS             complement(515..1549)
                     /locus_tag="VM910_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI64028.1"
                     /translation="MKLPKYVKAWVDKRDDRAYYYLRRRGFPRVRLPGLPWSPSFMAA
                     YESALAGPRAAIGAGRVKPGSVAAVVAEYLDSRQFFGSKSAGTQRMRRGILERFRAAY
                     GERPMALLPPEWIEALLDSKPPHAARSWLVTLRSLCHFALKPPRRYLRADPTANIKLA
                     SIKGDGFHTWTEDEIAQFEAHHPVGTKPRLALALLLYTAQRRSDVVRMGRQHIRETDD
                     GPALYVKQRKTGVELLIPIHPELGAVLDATPSEHLTFLVTATGKPYGDNHFSESFREW
                     CDAAGLPKRCSAHGLRKAACRRDAEAGCSANEIMAHSGHATMKELVRYTKAADQARLA
                     RNALAKAVIR"
     gene            complement(1546..1923)
                     /locus_tag="VM910_15810"
     CDS             complement(1546..1923)
                     /locus_tag="VM910_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64029.1"
                     /translation="MTDQASNIDLLATLGVPGAGRTRAVLKPIETILSDAPRAVRDHF
                     LAATKEALRLDATPAKPRRQRTPRRPTLASVAKQANKAALDVGRYEVKPDGTIVVVTG
                     QPEHAEPENAWPLDEFRTKETKQ"
     gene            complement(2266..3936)
                     /locus_tag="VM910_15815"
     CDS             complement(2266..3936)
                     /locus_tag="VM910_15815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVI64030.1"
                     /translation="MAFPYRKKISLVAAAMLAPLIAAGPAMAQKKPNIVMLMTDDTGW
                     NDFGAYTGGGAALGHPTPNIDRIAKEGAVFTSWYGQASCTAGRASFMTGRIPIRSALS
                     IVVAPGDENALKKETPTIAEFFQKNGYSTYFSGKWHLGDKPAAYPIEHGFDEMKAFAA
                     YYPGVYTYADTSKWFHPWFPSYNAEFAKDYFDVVNMYEWEGEAGKPAKRGEYITYDNL
                     ATFDVRQADFAIEYIKKHAKDDKPFFMDVNFMKMHNPTNAAPKFAGKSRLGDYSDSLL
                     ELDDNIGRVMDAIRAEAPNTIVIVTADNGAWQDAYPDAGTTPFRGEKGTAFEGGWRVP
                     GLMWWPGHIPAGAKYDEMMSHIDGWATLAKMVGLTPPPHGAWTDNNGKPIYFDSIDNS
                     AYILGQAKHSARRSWVYIDGESFMGARVDLGGDPNNPDLNIAWKYLWTAKDTWLGPEQ
                     NLGAIGATYNLTMDPFEKYDMTFNGAVASRLPNPSPGNYAGQDNGWLLALIYPVVMQF
                     NKSIVDYPSIKRSPGGASNDWRPNLQNPDNPVPLIDVKNPPKVKAGGG"
     gene            complement(4240..4389)
                     /locus_tag="VM910_15820"
     CDS             complement(4240..4389)
                     /locus_tag="VM910_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64031.1"
                     /translation="MATVSCGGMADDNPARVARANFRSTLPQPRRGIPLPLPVFTFNA
                     RQRFQ"
     gene            complement(4712..5857)
                     /locus_tag="VM910_15825"
     CDS             complement(4712..5857)
                     /locus_tag="VM910_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI64032.1"
                     /translation="MSGVQDDTGETKFRHLLDAAPVMVWVSAPDKLCTFFNKPWLSFT
                     GRTMEQELGYGWVEGVHPDDLALCYDIWSNEWERREPFQADYRLRRADGEYRWILDNG
                     VPYFAADGTFLGYIGSCIDMTDRKRAEAEARESERRYREVQMELAHANRLATIGQLTA
                     SITHEVTQPVAGAIVSAQAALHWLARDPPNLDEARQALTRVVKDGNRVSEVIAGIRAM
                     VKKAPARKHCLPVNEAIIEVVALTHGETIKNGVSVQTQLAEGLPLIEGDRVQLQQVML
                     NLIINAVEEMSGATEGARDLLISTGRAGSNDVLVSVRDSGPGLEPAALERLFDPFYTT
                     KSSGLGLGLSISRSIIEAHGGRLWATSNVPRGAIFEFTLPAYSDSKS"
     gene            complement(6110..6682)
                     /locus_tag="VM910_15830"
     CDS             complement(6110..6682)
                     /locus_tag="VM910_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64033.1"
                     /translation="MSRKNVFDGVFASSFRAEDHDVVATRRTLLLAVLAAGLPLAASI
                     RTAQASRLNPAETIVTLPDATKFVAWSGAPPHSGEMATLYGGLDTPGPYLALMKWYPG
                     YMSAPHSYATDRLSLVLSGTWSVNSGADFDPNQTVPVAAGGFVRRVAHTPHYDGVKRD
                     AKEPAVIALFGIAPVDLTLVDPSKPGWRQV"
     gene            6980..>7550
                     /locus_tag="VM910_15835"
     CDS             6980..>7550
                     /locus_tag="VM910_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVI64034.1"
                     /translation="MARSSPLWPSAPGAILRYAVAVLAVAAAAVAGLLLDRLLQTVPF
                     VSLFLCATLFAAWLGGAGPGLLATGLSILAFYYYFIPPTDSLVAQDIPRLALFAMTAL
                     FVVWVSVAQRRTAESLRRARDDLRATVQELENLNKSLQVENAERKKAEQSIRQAEREL
                     QVTIDTIPALATRYRRDGSLDFVNRTWRTY"
BASE COUNT         1345 a         2348 c         2347 g         1510 t
ORIGIN      
        1 tgagcccact cgacgtgcgc gacgcaggcg agatcgagcg cgtcatcgcg ttatttggac
       61 gctcctcgga tggcggcctg atcgtgacag ggagcgcatt ggcggcggct catcgcaaac
      121 tgatcgtcgc gctcgcggcc ccccaccgat tgccggcggt ctatcctttc cgctatttcg
      181 ccactagcgg cggcttgatc tcctatggac ctgatacgac cgacccattc cgccgcgcgg
      241 ccggctacgt cgctcgcatc ctcaacggcg agaaaccggg tgacctgccg gtgcaggcgc
      301 caaccaaata cgagcttgtc atcaatctca agaccgccaa ggcgcttggc cttgaagttc
      361 ctcccacgct gctcgcccgc gccgacgagg tgatcgagtg aaccgcgaac gaaagacgaa
      421 tccaaagtgt caaactcgac cggggtttga cactttcagt gcaagaagtg ccgatttgac
      481 gggcttaaaa atgagacgaa taactcgcgc cgggtcatcg gatgaccgcc ttcgccaacg
      541 cattgcgggc gagccgcgcc tgatccgccg ctttggtgta gcggactagt tctttcatcg
      601 tcgcgtgtcc gctgtgcgcc atgatctcgt ttgcgctgca tccggcctcg gcgtctcggc
      661 ggcaggccgc cttgcgcagc ccgtgcgcgc tacagcgctt cggcaatccg gcggcgtcgc
      721 accattcgcg aaagctctcg ctgaaatggt tgtcgccgta aggcttgccg gtcgcagtga
      781 ccaagaatgt cagatgctcg ctcggcgtgg cgtcgagcac cgcgcccaac tcaggatgaa
      841 tcggaatcag gagctcaaca ccagttttgc gttgcttgac gtacagggct ggcccgtcgt
      901 ccgtctcgcg gatgtgctgc cgccccatgc gcacgacatc ggagcgccgt tgtgcggtgt
      961 agagcaacag cgcgagcgcg aggcgcggct tcgtgccgac cggatgatgc gcctcgaatt
     1021 gcgcgatctc atcctcggtc cacgtatgga agccgtcgcc cttgatcgac gcgagtttga
     1081 tgtttgccgt cgggtccgcg cgcaggtagc ggcggggagg cttgagcgca aaatgacaga
     1141 gcgagcgcag cgtcacgagc cagctacgcg cggcgtgcgg cggcttgctg tcgagtagcg
     1201 cctcgatcca ttccggcggc agtaacgcca tcggccgctc gccataggca gcgcggaagc
     1261 gttctaagat tccccggcgc atgcgttgcg tgcccgcgct tttcgagccg aagaattgtc
     1321 gcgagtcgag atactcggcg acgactgcgg ctactgatcc gggcttgacg cggcccgcgc
     1381 cgatggcagc gcgcggacct gcgagcgcgg actcataggc ggccatgaag ctcggcgacc
     1441 acggcagacc gggaagccgc acgcgcggaa agccacggcg tcggagatag taataggctc
     1501 ggtcgtcgcg cttatcgacc cacgccttca catacttcgg caatttcatt gtttggtttc
     1561 cttcgtgcgg aattcatcga gcggccacgc gttttccggc tcggcgtgct cgggctgccc
     1621 ggtgacgacg acgatggtgc cgtcgggttt cacttcgtag cggccgacat cgagcgccgc
     1681 cttgttcgct tgcttcgcga cggaggcgag cgtcggccgt cgcggtgtgc gctggcggcg
     1741 cggctttgcg ggcgtggcgt cgaggcggag agcttccttg gttgctgcaa gaaagtgatc
     1801 gcggacggcg cgcggagcgt ccgataggat tgtttcgatc ggcttgagca cggcgcgagt
     1861 ccgaccggcg ccgggtactc cgagcgtggc gagtaaatct atgttagagg cttggtcggt
     1921 cacgcggcgc tgctcctgac agcgttgttt gatcgtgccc caaggtgccg cgacagcacc
     1981 ttggggcgaa ctcatttttc gccggccccg cagccacgtc aagaaatttc cgcaccggtg
     2041 ctgcgcggtg atcccgctgc ggggatgacc cttggctcgg gcgtgtattc ataaatcgac
     2101 gacgctgatc ggtgtccgct tagcccccac aagcggcgca aaagcggaca tttcctaacc
     2161 gccgcttcgt gctgccacaa gcagacatca gcacaatgtc gcgaatttca aaaatatagc
     2221 ggggcatgtt acgcgcatgc cccgctccta tgatgtaggc tacgactagc cgccgccggc
     2281 cttgaccttt ggcggattct tcacatcgat caacggtacg gggttgtccg gattttgaag
     2341 attgggcctc caatcgttcg aggctcctcc gggggatcgc ttgatgcttg gataatccac
     2401 gatcgacttg ttgaactgca tcacgactgg gtagatcagc gccaagagcc agccgttatc
     2461 ttgccctgca tagttgcccg gcgaggggtt cggcaggcgc gaggcgaccg ctccgttgaa
     2521 cgtcatgtcg tatttctcga acgggtccat tgtgaggttg taagtcgcac caatcgcgcc
     2581 caggttctgc tccggaccga gccacgtgtc cttggccgtc cataggtatt tccacgcgat
     2641 gttgagatcg ggattgtttg gatcaccgcc gaggtccacg cgcgcgccca tgaaactttc
     2701 gccatcgatg tagacccatg atctccgggc agagtgcttt gcctgcccga ggatgtaagc
     2761 gctattgtcg atgctgtcga agtagatcgg cttgccgttg ttgtctgtcc acgcaccgtg
     2821 aggcggcggg gtaagaccga ccatcttggc tagcgtcgcc cagccgtcga tgtgcgacat
     2881 catttcgtcg tacttcgcgc cggccgggat gtgtccgggc caccacatca agcccggtac
     2941 gcgccagccg ccttcaaatg ccgtgccctt ttcgccgcgg aagggcgtgg taccggcgtc
     3001 ggggtaggcg tcctgccaag cgccgttgtc ggccgtcacg atgacgatcg tgttgggtgc
     3061 ctccgcgcga atggcatcca tgacccggcc gatgttgtca tcgagttcca agagcgaatc
     3121 cgagtaatcg ccgaggcgcg acttgccggc aaacttcggt gcggcattgg tcgggttgtg
     3181 catcttcatg aagttgacgt ccatgaagaa cggcttgtcg tccttggcgt gcttcttgat
     3241 gtactcgatt gcgaagtcgg cctgccggac gtcgaaggtg gccaggttgt cgtaggtgat
     3301 gtactcgccc ctcttcgccg gtttgccggc ctcgccctcc cattcgtaca tgttcacgac
     3361 atcgaaatag tccttcgcga actccgcatt gtacgaaggg aaccacggat ggaaccactt
     3421 ggaggtgtcg gcgtaggtgt agacaccagg atagtacgcg gcgaacgcct tcatctcgtc
     3481 gaagccgtgc tcgattggat aggccgcagg cttgtcgccg agatgccact tgccagagaa
     3541 gtatgtcgaa tagccgttct tctggaagaa ttccgcgatg gtgggcgtct cctttttcag
     3601 cgcattctcg tcgcccgggg ccaccacgat cgagagcgcc gagcggatcg ggatgcgtcc
     3661 ggtcatgaac gacgcgcggc cggccgtgca actcgcctga ccgtaccaac tcgtgaacac
     3721 cgcgccttcc ttggcgatcc ggtcgatgtt cggggtcgga tggccgaggg ccgcgccacc
     3781 ccccgtatag gcaccgaaat cgttccagcc cgtgtcgtcg gtcatgagca tgacgatgtt
     3841 cggcttcttc tgcgccatgg ccggcccagc cgcgatgagc ggagccagca ttgccgcggc
     3901 aaccaaggaa attttcttgc gataggggaa cgccataacc tacctccatg tctttccgtt
     3961 tcggttagat ttcatggatt tcggcggcag accgcattcg gccggccgcc aactaggcat
     4021 aacacccctt gccggggggc tagttcaagc cccggtgact cgcgcttctc ggcaccgggt
     4081 agccgacgtc ggctgctacc tccgtcgcct acgcgagcgc cgaccatcga gaagcacgat
     4141 cgccgctaca acgacggccg gccacacgaa gcagtcgaga ccgaggcgct gcggcagagc
     4201 gtgctgcaac gcatcctgcg cgagcgcctc gatgcgctgc tactggaacc gctggcgcgc
     4261 gttgaacgtg aaaaccggca gcggcagcgg aatcccccgg cgcggctgcg gcagcgttga
     4321 ccggaagttt gcacgggcga cgcgcgcggg attatcgtcc gccatccctc cgcaggagac
     4381 cgtcgccatg ctccctgcct ccgacctccc cgaaatcatc ggcaacgtcg tcgcccttaa
     4441 tctcgacgct caaaccacca gcaatgccgg cggcgtgcag gcggtgcagt ggagcttgcc
     4501 cggctgagcg cccgagatcg gcgagcggcg tcaagctcgg gcgtgcactc ataaatcgcc
     4561 ccgtcgtgtc cgcttttggg caaaccggac attgagccga catcgccgaa tgaccgagtc
     4621 tgacccgaag cggacatctg tctggccaac cccatccccc tttgtgtgcc cgctggcata
     4681 cgccggaaaa tgacaggcgc aacttcaccg attacgactt actgtcggag taggcgggca
     4741 acgtgaattc aaagatggcg ccgcgcggca cgttggaggt tgcccacaat cgtccgccgt
     4801 gcgcctcgat gatcgaacga gagatcgaca gccccagccc caagccgctc gacttggtcg
     4861 tgtagaaggg atcgaaaaga cgctcgagag ccgctggttc cagtcccgga cccgaatccc
     4921 ggaccgagac gagcacatcg ttggaaccgg ctctcccggt gctgatcagc aagtctcgcg
     4981 ccccctcggt cgcaccgctc atttcctcga cggcattgat aatcaagtta agcatcactt
     5041 gttgcagttg cactcggtcg ccttcgatga gcggcaagcc ctccgcgagt tgcgtctgca
     5101 cagaaactcc gttctttatc gtttcgccgt gggttagggc gacgacctcg atgatcgctt
     5161 cgttgacggg caggcaatgc ttccgcgcag gcgctttctt gaccatcgca cggatgccgg
     5221 caatgacctc gctgactcga ttgccgtctt tgacgacgcg cgtgagcgcc tgccgcgcct
     5281 cgtccagatt gggcggatca cgggccagcc agtgcagcgc cgcctgggcg ctgacaattg
     5341 cgccggcaac gggctgggtg acttcatggg tgatggaggc cgtcaactgg ccaattgtgg
     5401 cgaggcggtt cgcgtgcgcc agttccatct gtacctcgcg atatcgccgc tcgctctcgc
     5461 gtgcctctgc ttcggcgcgc tttcggtcgg tcatatcgat gcaggagccg atgtagccta
     5521 ggaacgtgcc atcggcggcg aaatatggca cgccgttgtc gaggatccag cggtactcgc
     5581 cgtcggcgcg tcgcaaccga tagtccgctt ggaagggctc gcggcgttcc cattcgttgg
     5641 accagatgtc gtagcagagc gccaaatcgt ccggatggac gccctcaacc caaccgtagc
     5701 cgagttcctg ctccatggtg cggccggtga aggagagcca cggcttgttg aagaaggtgc
     5761 agagcttgtc gggtgccgac acccagacca tcaccggcgc agcgtccagc aagtggcgga
     5821 attttgtctc gcctgtatcg tcctggacgc ctgacatgct gttttctgct gccacgattt
     5881 gcacaggtaa ccccaaacct attatggagc caaggatgag gcaccctaaa atatatatat
     5941 gtatcggccg tgtggcgcgt ttctatctcc tgagaggcta acggcgactt ccggttttgg
     6001 cacttctcgg acctcgggaa ctgtccgatt tgagtccgca aagtggtgcc aaagcggact
     6061 tgatcaggtc gctgtctcca atcgcgattt tatgagtaca cgccgtaatt caaacctgcc
     6121 gccagcccgg cttactggga tccaccagcg tcagatcgac cggcgcgatt ccaaacagcg
     6181 cgatcaccgc tggctccttc gcatcgcgct tcacgccgtc gtagtggggc gtgtgagcga
     6241 cgcgccgcac gaaccctccg gccgcgaccg gcacggtttg attggggtcg aagtcggcgc
     6301 cgctgttcac cgaccacgtg ccggaaagga cgagcgagag acgatcggtc gcgtaggaat
     6361 gcggcgcgct catgtagccg ggataccatt tcatcagggc gagataaggt cccggcgtgt
     6421 ccaagccgcc gtagagcgtc gccatttcgc cgctgtgcgg cggagcaccg ctccaagcaa
     6481 cgaacttggt ggcgtcaggg agcgtgacga tggtctcggc gggattaagc ctgctcgcct
     6541 gcgcggtcct gatcgaggcc gcgagtggca aaccggcagc caacacggca agcaacagtg
     6601 tccggcgcgt ggcgacgacg tcgtgatctt cagcgcggaa gctggatgcg aacactccgt
     6661 cgaaaacatt tttgcgcgac attggagtct ccatctttgc gagcgtcgac gttcagcccc
     6721 cgctccgatg accgagcgcc cagacgtagg cggcgacgac ccgacggctc ggaggaggac
     6781 agctgggcac cgccgatgac ctacggctgc agggccgaag cggcttgcgc ggaattgccg
     6841 ccattgccgg gatcttgcgg caatatcgat gaggccgttc agcgctcaga tcggcgttat
     6901 caatatccgg tgacgcgctt cctgcgctgt tcccgctggc atgctaacat cgccagacgc
     6961 tggagctgga gccggaagca tggcgcgatc cagtccgctg tggccatccg cgccgggcgc
     7021 gatcctgcgc tacgcagtcg cggtcctggc ggtcgcggca gctgccgtcg ctgggttatt
     7081 gctggacagg cttctgcaaa ccgtcccgtt cgtgtccttg tttctgtgcg cgaccctgtt
     7141 cgccgcgtgg cttggcggcg ccggccccgg tctgctcgca accggactct ccattctggc
     7201 tttctattac tatttcattc ctccaacgga ttcgcttgtc gctcaggaca taccgcgcct
     7261 cgcccttttt gccatgacgg cgcttttcgt ggtttgggtc agtgtagcgc aaaggcgcac
     7321 ggcggaatcc ctgcgacgcg cgcgcgacga tttgcgggcg accgttcaag agctggaaaa
     7381 cctcaacaaa tcgctgcaag ttgagaacgc cgaacgaaag aaagcggagc aaagcatccg
     7441 ccaagccgag cgagagcttc aggtaacgat cgataccatt ccggcattgg cgacgagata
     7501 tcgacgcgac ggctcgctcg atttcgtgaa tcggacctgg cgaacctata
//
LOCUS       DATLJT010000688         1912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1880462,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000688 DATLJT010000000
VERSION     DATLJT010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880462"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VM910_15840"
     CDS             complement(<1..288)
                     /locus_tag="VM910_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HVI64035.1"
                     /translation="MKRIDHAEAASEFIAARAQQEFHDRRLWDLRLKRDREACVIPEW
                     EELRNLASQIKEHTLSRLADYLEQFESRAKENGAHVHWARDAAEHNQIVYDI"
     gene            complement(285..1028)
                     /locus_tag="VM910_15845"
     CDS             complement(285..1028)
                     /locus_tag="VM910_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVI64036.1"
                     /translation="MKVGLFIPCYVDAFFPAVGIATLQLLERLGLEVVYPLDQTCCGQ
                     MMANSGSQKDAAGAEALFVENFKDFDHIVTPSGSCAYHVREHLDAIPQTEEAQKVRSN
                     TYELVEFLHDVLKVEAFPWAEFPHKVGLHNSCGTLRKLRTAKASEIDEPFFSKPLDLL
                     SKVRGIQFVTPSRPDECCGFGGTFSIFEEPVSAKMGYDKVADHHAAGAEYIVSADMSC
                     LMHQKGCAERVGLDIRFIHIAQILNGDRA"
     gene            complement(1127..>1912)
                     /locus_tag="VM910_15850"
     CDS             complement(1127..>1912)
                     /locus_tag="VM910_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVI64037.1"
                     /translation="EVLVAPPRGEVIKAWIPWLILSVAVILWGTPQFKALLDNLSIVR
                     IPVTGLHNLVERVPPVVLRPAPEAAVYLFNWLSATGSGILVAAVVAGLVMGFSPVGLV
                     RTYWNTLKLVRYSLLTIACMLAIGFITRYSGIDATLGLAFANTGPLYPLFGTMLGWLG
                     VALTGSDTSSNVLFGSLQRITAEQLGLSPTLMSAANSSGGVMGKMIDAQSIVVASTAT
                     RWYGHEGDILRYVFLHSLALAVLVGLLVSLQAYVWPFTALVVR"
BASE COUNT          406 a          572 c          582 g          352 t
ORIGIN      
        1 aatgtcgtag acgatctgat tatgctcggc ggcgtcgcgg gcccagtgca cgtgcgcgcc
       61 gttctctttc gcccggcttt cgaattgttc gagatagtcc gcgagccgtg acagcgtgtg
      121 ttccttgatt tgcgaggcaa gattgcgcag ctcctcccat tccggaatta cgcacgcctc
      181 gcggtcgcgc ttgagcctga gatcccaaag ccgccggtcg tggaattcct gctgcgccct
      241 ggcggcgatg aattcggagg ctgcctcggc atgatcgatc cgcttcatgc gcgatctccg
      301 ttgaggattt gcgcgatatg gatgaagcgg atgtcgagcc cgacccgttc ggcgcagccc
      361 ttttggtgca tgaggcacga catgtctgcg gaaacaatgt attcggcgcc agcggcgtga
      421 tgatcggcaa ccttgtcgta acccatcttc gccgaaactg gctcctcgaa aatggaaaag
      481 gtgccgccga agccgcagca ttcgtcgggc cggctcggcg ttacgaattg aatgccgcgc
      541 accttgctga ggagatcgag tggcttcgaa aaaaacggtt cgtcgatttc ggatgccttt
      601 gcggtgcgaa gcttgcgaag cgtgccgcaa gaattgtgaa ggccgacctt gtgcggaaat
      661 tcagcccagg ggaaggcttc gaccttgagc acgtcatgca ggaattcgac cagctcgtaa
      721 gtgttgcttc gcaccttctg cgcctcctcg gtctgcggga tcgcatccaa atgttcccgc
      781 acgtggtagg cgcagcttcc cgacggtgtg acgatgtgat caaagtcctt gaagttctcg
      841 acgaacaatg cctccgcccc agctgcgtcc ttctgactgc cgctgttcgc catcatctgg
      901 ccgcagcagg tttgatcgag cggatagacc acctcgagac ccagccgttc caatagctgc
      961 agcgtggcga tcccgaccgc cggaaagaag gcgtcgacat aacagggaat aaacaatccc
     1021 actttcatga tcgatgctcg tcaagccgac attccaccac agcagctatc aacgcgatgc
     1081 cggccgacca ctctcgatcg gctggagaca cggaatggca attgcgtcag cgcaccacca
     1141 aggcggtgaa tggccagaca tacgcctgaa gcgagacgag gaggccgacc aggacggcca
     1201 gggcgagtga gtgcaagaac acgtagcgca ggatgtcgcc ttcatggccg taccatcgcg
     1261 tcgccgtgct cgccacgacg atgctctggg catcgatcat tttgcccatg acgccgcccg
     1321 agctgttcgc cgcagacatc aaggttgggc tgagccccag ctgttcggcc gtaatgcgct
     1381 gcaggctgcc gaacagcacg ttggacgacg tgtcggaacc ggtgagtgcc acgccgagcc
     1441 agcctagcat cgtgccgaac agcgggtaga gtgggcccgt gttggcaaag gcaagaccga
     1501 gcgtcgcatc gataccggaa tagcgggtaa tgaatccgat tgcgagcatg cacgcgatcg
     1561 tcaacagcga gtagcgcacg agcttgagcg tgttccaata ggtgcgcacc aggcccacgg
     1621 gcgaaaagcc catgaccaag ccggcaacaa cggcggccac gaggatgcca gagccggtgg
     1681 cggacagcca attgaataga tagacggcgg cctcaggcgc cggtctaagc acgacgggag
     1741 gcacccgctc gaccaagtta tgcagaccgg tcacgggaat gcgcacgatc gagaggttgt
     1801 ccagcagcgc cttgaactgc ggagtgcccc agagaatcac ggccacgctg agaataagcc
     1861 aggggatcca cgctttgatc acctcacccc gcggcggcgc aactagcacc tc
//
LOCUS       DATLJT010000689         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_730636,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000689 DATLJT010000000
VERSION     DATLJT010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730636"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1171
                     /locus_tag="VM910_15855"
     CDS             416..1171
                     /locus_tag="VM910_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI64038.1"
                     /translation="MDGTIVNVAIYLTATFAAALVTGVAGFAFGLVAAGVWLHILTPI
                     QTATLIIAFGLVVQGISVWKLRHALRWSRLWPFLLGAAFGVPLGVAILGWARPDYVRV
                     TVGVVLVLYSTYSLARPAMKPVKRGGATADAGVGFLNGILGGLTGLAGILTTIWCGLR
                     GWLKDEQRAIFQPIGVAIFAMSGLWLGINGAVSMDTVWFFVIGLPALLAGTWLGLKLY
                     GHLDEAGFRKLVLVLLLVSGLALVAPALAPLLG"
     gene            complement(1201..>1525)
                     /locus_tag="VM910_15860"
     CDS             complement(1201..>1525)
                     /locus_tag="VM910_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64039.1"
                     /translation="LAAFSPNLTIVLALLLVRSALSQMDVPTRTSYVMAVVTPAERTA
                     AASVTAVPRSLASSISPAISGALLSTSFAGLPLVVCGVLKIAYDLSLLFSFRHIKPPE
                     EKEAP"
BASE COUNT          270 a          439 c          502 g          314 t
ORIGIN      
        1 gcttgcggcg agtaacgcaa cgccgatcac gcttgcgatg agtcgattca tgtctctctc
       61 cttctggtac atggtccatc gacgggcaga gcaaagcggc gaccatttaa ggcaaaatta
      121 aacggcgact tgggtcgatc acagaacggt gagggctgcg acttcgtgcc gtcgtacagc
      181 accggcgctc gtgaatgttt gctcgaaacg tgagcgagga tcgcgcaagg tgccgcgccg
      241 gcttgagctt taaaactgcc ttaaacaagc gctctcagcg taagggactt tcgattggtg
      301 caaacattcg cgtttgccga caggcgacaa gtacgcccgt caagtcgaaa gcgggaggtg
      361 acggctgtcg atagcgaggc tgccagtcgt ccacgcatag ccgggtgagc gaccgatgga
      421 tggaacgatc gtcaacgttg ccatttatct gacggcgacc tttgctgccg cgcttgtcac
      481 gggggtggca gggtttgcat tcggattggt tgccgccggc gtttggctgc acatcctcac
      541 gccgatccag accgcgacgt tgatcatcgc cttcggtctt gtcgtgcagg gcatctcggt
      601 ctggaaattg cgccacgcgc tgcgctggag tcggctgtgg ccgttcctgt tgggggcggc
      661 ctttggcgtc ccgttgggcg tggcgattct cggatgggcg cgtcctgact acgtccgcgt
      721 caccgtcggc gtggtgctcg tgctctacag cacctatagc ctcgcccggc ccgcgatgaa
      781 gcctgttaag cgcggcggcg caacggcgga tgcgggcgtg gggtttctga atggcattct
      841 cggcggcctc accggcctcg ccggcattct cacgaccatc tggtgcggcc ttcgtggttg
      901 gctcaaggac gagcagcggg cgatcttcca gccgattgga gtggcgatat tcgccatgag
      961 cggtctgtgg cttgggatca acggtgcagt atcgatggac accgtttggt tctttgtcat
     1021 tggcctcccg gcattgctgg ccgggacgtg gctcggacta aagctttacg gacatctcga
     1081 tgaagccggc tttcgcaagc tggtgctggt cctgttgctg gtctcgggac ttgcgctggt
     1141 cgcacccgct ctcgcaccgc ttttaggttg acggtgcacc ggacgagcaa catgacttga
     1201 tcagggcgct tccttttctt ccggcggctt gatgtgtcga aacgaaaaca agagcgagag
     1261 gtcgtaggcg atcttcaaca cgccgcagac gacgagcggc aatcccgcaa acgacgtcga
     1321 cagcagagcg ccggatatcg ccgggctgat cgacgaagcc aagctgcgcg gaaccgcggt
     1381 cacgctcgcg gccgccgtcc gctccgcggg ggtcaccacc gccatgacgt aggacgtgcg
     1441 cgtcggcacg tccatctgcg agagcgcgga gcgcacgagc agcagcgcga gcacgatagt
     1501 aagattcggc gagaaggctg cgaga
//
LOCUS       DATLJT010000690         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_502549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000690 DATLJT010000000
VERSION     DATLJT010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VM910_15865"
     CDS             complement(<1..549)
                     /locus_tag="VM910_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVI64040.1"
                     /translation="MAVFDITACAALREDITPIVARAAAVIRQCGPSALRQKPDGSPV
                     TAADEAAEAVILRGLERLAPNIPIISEERAAPSAGSTGEVYFLVDPLDGTREFIAGRD
                     EFAVNIALINRGTPLMGIIAAPAADLVWRGIVGGGGAERMVLSDQSRRTAIRTRPRPA
                     HPVIMISRSHLDAGTEAYLARVP"
     gene            complement(590..2020)
                     /locus_tag="VM910_15870"
     CDS             complement(590..2020)
                     /locus_tag="VM910_15870"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVI64041.1"
                     /translation="MDINRFTRVDATTWRIEPYGAMRVPAIIFADEKLIRDMDDKVHE
                     QATNVATLPGIVQASYAMPDAHWGYGFPIGGVAAFDPDKGGVVSAGGVGFDISCGVRT
                     MLTGLKISDVVPLQRSLADSLYRQIPAGVGSRGAITLDAIDMDAMLVGGAGWAVKQGW
                     GEDRDLERIEENGKMTGARPDYVSERAKERQRREMGTLGSGNHYLEVQAVTEIFDAKV
                     GEVFGLSIDDVVATIHCGSRGLGHQIGTEFLKEMTVAAKVSGIELVDRELACAPINSE
                     VGQRYLGAMRAAINCALANREILCHYTRRVFAHFFPKCDLTLLFDVSHNTCKVERHAV
                     DDRQRELFVHRKGATRAFGPGHPSLPDALRRVGQPVLIGGSMGTGSYILVGEATAEKK
                     AFASACHGAGRALSRHAALKQWSGRKIVDELAERGILIKSPSMRGVAEEAPGAYKDVS
                     AVVAAAERAGLARRVAHLRPLICIKG"
     gene            complement(2279..2770)
                     /locus_tag="VM910_15875"
     CDS             complement(2279..2770)
                     /locus_tag="VM910_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HVI64042.1"
                     /translation="MKRAAWEHFPHEADVGIRGFGVTAAEAFEQAAVALTAVVTHAEI
                     KPLIPVEVSCEAPDLELLFVDWLNAVIYEMAIRKMLFGRFAVAIEGTRLNGILWGEPV
                     DVARHAPACEPKGATYTALQVRRNDGGLWSAGCVVDVYTARNNGCTQNWPNGRNRVMS
                     RDC"
     gene            2961..>3537
                     /gene="pncA"
                     /locus_tag="VM910_15880"
     CDS             2961..>3537
                     /gene="pncA"
                     /locus_tag="VM910_15880"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HVI64043.1"
                     /translation="MVDVQNDFCPGGALAVPRGDEVVPIVNRLAENFRSVVLTQDWHP
                     AGHLSFASTHPGRSPYDTIAAPYGPQVLWPDHCVQGTRGAAFHDTLQIPHAGLVLRKG
                     LHRTIDSYSAFYENDRATPTGLTGYLRECGIKRVFLAGLALDFCVRYSAEDAVRGGFA
                     VAVIDEACRGIDVAGSVRATWLSFTALGVVTI"
BASE COUNT          666 a         1117 c         1021 g          733 t
ORIGIN      
        1 cggcacacgg gcgagatagg cctcggtgcc ggcgtcgaga tgcgagcggc tgatcatgat
       61 cacgggatgc gccggacgcg gccgggtgcg aattgcagtt cgccggcttt ggtcgctcag
      121 caccatacgc tcggcgccgc cgccaccgac gatgccgcgc caaaccaaat cggcggccgg
      181 cgccgcgata atgcccatga gcggagtgcc gcgattgatc aatgcgatat tgaccgcgaa
      241 ttcgtcacgt ccggcgatga attctcgggt gccgtcaagc ggatcgacca gaaagtacac
      301 ctcgccggtc gagccagcgg agggggcagc gcgttcttcc gaaatgatcg gaatattagg
      361 cgcaaggcgt tcaaggcccc gtagaatgac cgcttcggcg gcctcgtccg ccgcggtaac
      421 gggagagccg tcaggcttct ggcgcagcgc actggggccg cactgccgaa tgactgccgc
      481 cgcgcgagca acaatcggag ttatatcctc cctgagcgcg gcgcatgcgg tgatatcgaa
      541 gactgccacg gccgattact ggtttgcgag cgcgatcaaa ataccgcttt catcccttga
      601 tgcagatcag aggtcgcagg tgcgctactc gtcgcgccag tccggctctc tcggctgccg
      661 ccaccactgc gctcacatcc ttataggcac ccggcgcctc ttccgcgacg ccgcgcatcg
      721 acgggctttt gatgagaatg ccccgttcgg caagctcgtc tacaatcttg cggccgctcc
      781 actgcttgag cgctgcgtgt cgtgagagcg cccgcccggc gccgtggcat gcagaagcaa
      841 atgccttctt ttcagctgtg gcctctccga ccagaatgta cgaaccagtt cccatgctgc
      901 caccgatgag gaccggctgg cctacgcggc gcaatgcgtc cggtaagctc ggatgtccag
      961 gaccaaaggc gcgcgtcgct cccttacgat gaacgaacag ctcgcgctgc ctatcatcga
     1021 ctgcgtgccg ttcgaccttg caggtattgt gcgaaacatc aaacagcaag gttagatcgc
     1081 atttgggaaa gaaatgggca aaaacacgtc gggtgtaatg gcagagaatt tcgcggttgg
     1141 ctaacgcgca attgatggct gctcgcatcg cgccgaggta gcgctggcca acttctgaat
     1201 tgatcggcgc gcaggcaagt tcccggtcaa cgagctcgat gcccgatact tttgccgcta
     1261 ccgtcatttc cttcaagaat tcggtgccaa tctgatgacc aagcccgcgc gagccacaat
     1321 gaatggtggc aacaacatcg tcgatggaaa gtccgaatac ttcgccgact tttgcatcga
     1381 aaatctccgt tacggcttgg acttcaagat agtggttgcc ggaaccgagc gttcccatct
     1441 cccttcgttg tcgttccttg gcgcgttcgg agacgtaatc tggacgggcc ccagtcatct
     1501 tcccgttttc ctcgatccgt tcgagatcgc gatcttcgcc ccaaccttgc ttcacggccc
     1561 aacccgcgcc tcccaccagc atcgcgtcca tgtcgattgc atcgagcgtg atcgctcccc
     1621 ggctaccgac accggctggg atttggcggt ataacgagtc tgccagcgat cgctgcagcg
     1681 ggacgacatc agaaatcttg agcccggtca gcatagtgcg cacgccgcat gaaatgtcga
     1741 agccaacccc gccggccgaa acgacaccgc ccttgtcggg atcgaacgca gccacaccgc
     1801 cgattggaaa accgtagccc cagtgagcgt ctggcatcgc ataggaggct tggacgatgc
     1861 cgggcagtgt tgcgacattc gtcgcctgct catggacttt gtcgtccatg tcgcggatca
     1921 gtttttcgtc tgcgaaaatg attgcgggca cgcgcatggc gccatacggc tcgatgcgcc
     1981 aggttgtcgc gtcgacgcgt gtgaagcggt ttatatccat gcgggcgatc ttttttgttc
     2041 gaactttgct cgaagctcag ccaatgctgg ccatacgatg cctggcattc ggcgagtgtt
     2101 attttgtgtt attcgcgttg ttttgccggt ggtgcaaccc cactccttag cgagcctgcg
     2161 cttaacggcg agcgcgccca tccgagacaa ttctttgtcg gcgtgtgctc gtacgataac
     2221 tatccttggt tcagcgatac cagtggctcc gaccaccgcc aagggttcga ctactacatc
     2281 aacaatcacg ggacatcact cggtttctgc cattcggcca gttctgggta cagccattgt
     2341 tgcgggccgt atatacgtcc acgacacaac cagccgacca cagccctcca tcattcctcc
     2401 taacctgtaa tgccgtgtaa gtcgcgccct tcggctcgca ggcaggcgcg tgccgcgcca
     2461 catcaacggg ctcgccccac aatatcccgt tcaatcgcgt gccctcgatc gccacggcaa
     2521 agcgcccaaa cagcatcttg cgaatggcca tttcgtagat cacagcgttg agccaatcga
     2581 caaacaggag ctccagatca ggcgcctcgc agctcacctc gacggggatc agcggcttga
     2641 tctcggcatg cgtcacgacg gcggtcaggg cgaccgccgc ctgctcaaat gcctctgccg
     2701 ccgtgacgcc aaagccgcgt atgcccacgt cagcctcatg gggaaaatgc tcccatgctg
     2761 cgcgtttcat acgcggctcg accggcatgc tcgcagccac cagaccaaac ctttccctgg
     2821 atccttcatc tttgtccgtt ccattcccag caggatgttg acagccggct gcgcggggtt
     2881 ttgagcttgg gtaaccgcgg agtttcggga gatcaagtct gtccacagct tgtgcaatcc
     2941 gcgacagcga tgtcctcatc gtggtcgatg tgcagaacga cttctgtccc ggcggcgcgc
     3001 tggctgtgcc gcgcggcgat gaagttgtgc cgatcgtcaa ccggcttgca gaaaatttcc
     3061 gcagcgtggt cctcacacag gattggcatc cggccgggca cctgtcgttt gcctcaacgc
     3121 atcccggccg ttcgccctac gacacgattg cagcgcccta cggaccgcag gtgctgtggc
     3181 cagaccattg cgtgcagggt acgcgcggcg ctgcttttca cgacacacta caaattcccc
     3241 atgccgggct ggtgctgcgc aagggactcc accggaccat cgattcgtac tcggcgttct
     3301 acgagaacga tcgcgcgacg ccgactggcc ttacgggcta tcttcgcgag tgcggcatca
     3361 agcgcgtatt tctggccggc ctggcgctcg atttttgcgt ccggtattct gccgaagacg
     3421 cggttcgcgg gggcttcgcg gtcgcggtca tcgacgaagc ctgccgcggg atcgacgttg
     3481 cgggttcggt acgggcgacc tggttgagct tcactgcact cggcgtggtc acaattg
//
LOCUS       DATLJT010000691         1508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2570860,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000691 DATLJT010000000
VERSION     DATLJT010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570860"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VM910_15885"
     CDS             complement(<1..249)
                     /locus_tag="VM910_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI64044.1"
                     /translation="MSLIYRLASRNLLHDRLRFVATVVGIVFSIVLVTVQLGLYLGFG
                     RVVTTMIDHASADLWIMPAGTKCFEDPSTLDERKRFQAL"
     gene            630..1187
                     /locus_tag="VM910_15890"
     CDS             630..1187
                     /locus_tag="VM910_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI64045.1"
                     /translation="MLLVASLVAFTSATLPTQAAEPTVAGLWEKKDESGKSMGWFLFV
                     ERNGVFEGAFAKLWARPGDAPNPTCTGCTDDRRNAPLLGMSFIRGMQRSGLRYDNGNI
                     TDPRDGSVYRAMMTLSPDGQVLTVRGYLGIPLLGKDEVWQRLPDSAMAQVDRSVVAKY
                     LPGRAPAAAARPAPAAVAKGSAAAR"
     gene            complement(1277..>1508)
                     /locus_tag="VM910_15895"
     CDS             complement(1277..>1508)
                     /locus_tag="VM910_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64046.1"
                     /translation="QPMEKPRDRSILEATFKMINAETQLMWSSDYPHWDFDLPSVIYD
                     LPFLSESGRRNILGGTAAKVFGLDPRPVKKIP"
BASE COUNT          291 a          463 c          483 g          271 t
ORIGIN      
        1 cagcgcttga aatcgcttgc gctcgtccag cgttgacgga tcttcgaagc acttcgttcc
       61 cgccggcatg atccacagat ccgccgatgc gtggtcgatc atcgtggtga ctactcgtcc
      121 gaatcccaaa tagagaccca gctggacggt cacgagcacg atggaaaaga cgatcccgac
      181 gacggttgcg acgaatcgca accgatcgtg caacagattc cgcgatgcga gccggtagat
      241 cagcgacatg ggcggacgcg gttgctcagc gttcaaactg ataatgatga agggtcgaag
      301 ttatcgagca cagccatcaa agggccgcgc atgattgcct cgcgcgcgcg gcaaaacaag
      361 gctcggtctt cgacaaaaaa ggcggatggt tgacggcgat gagccccgcg gaccggccgc
      421 tcgaccgtgg aaaaggaggc caaatcgcgg caaggcacga gattttttcg actcccgggc
      481 cttattgcgt caattcgcgg cgcggggtcc gcgaaaccga cgggtatcgc gcggcggcgc
      541 cctgaaatgg gcatattgtt gagagaattc cgtagaccat cacgccgctg atttagacgg
      601 gggtcactcc attgaaaaca cgattgtcga tactgctcgt cgcgagcctc gtggcgttca
      661 cgagcgctac ccttcccacg caagccgcgg agccgaccgt cgccggcctg tgggagaaga
      721 aggacgagtc cggcaagtcg atgggatggt ttctgttcgt ggagcgcaac ggcgtcttcg
      781 agggcgcgtt tgcaaagctg tgggcgcggc ccggcgacgc ccccaatccg acctgcaccg
      841 gatgcacgga cgatcgcagg aatgcgccgc tgctcggtat gtccttcatc cgcggcatgc
      901 agcgcagcgg attgcgctac gacaacggta atatcaccga tccacgcgac ggtagcgtct
      961 accgcgccat gatgacgctc agccccgacg gccaggtgct gaccgtgcgg ggttatctcg
     1021 gcattccgct gctcggcaag gacgaggtgt ggcagcgcct gcccgacagc gccatggcgc
     1081 aggtcgatcg ctccgtcgtc gccaaatacc tgccgggacg cgcgccggcg gctgcggcgc
     1141 ggccagcccc cgccgccgtt gcgaagggaa gcgctgccgc gcgctgacgg ctttccgcca
     1201 gtattcgctt ggcatcgcgc gtcgctgatt cgcgttgcgg agaattaact ccgtaacgcg
     1261 agaaaatcgg ctcccgtcac ggaattttct tcaccggcct ggggtcgagc ccgaatacct
     1321 tggctgcggt accgccgagg atgttgcggc ggccgctttc gctcagaaac ggcaggtcgt
     1381 agatcacgct cggcaggtcg aagtcccagt gcgggtagtc cgacgaccac atcagctggg
     1441 tctcggcgtt gatcatcttg aacgtcgcct cgaggatcga gcgatcgcgc ggcttctcca
     1501 tgggctgc
//
LOCUS       DATLJT010000692         1964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3718726,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000692 DATLJT010000000
VERSION     DATLJT010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3718726"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VM910_15900"
     CDS             <1..280
                     /locus_tag="VM910_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI64047.1"
                     /translation="TRMQVFRKLVVPSCMPWIISVLRVNIGLALTGAIVGEFIASQHG
                     LGRAILYAGQTYDIALVWVAVLVLSTLAVVMYAAVSWLEAWLRKGVRQ"
     gene            318..1316
                     /locus_tag="VM910_15905"
     CDS             318..1316
                     /locus_tag="VM910_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64048.1"
                     /translation="MNRRKFLKSTAALGLASMAQPSIPRAASREIVVAEPVHGTGYLP
                     LYIGMANGYFAEADVTVQILTIETGAGHTNAVLSGQAFAFIGGPEHNAFAKAKGAELR
                     AVVHCVDRGNVYYCAAKGQEPANSDWPGYFKGKSIALSQFGGTPNSITRYLLKKWNLD
                     PKSDVTLVEVANSAVLSVVKSKQAQIGVSTEPFVTQGVRQSIWSEPFYNIPKQLGPYA
                     YSTLNVRLDTIQKEPELVRAFVRGMVKGLKFTYAHPDQAAEIAKKQFPTMPLEDLKAT
                     LDRSFRDEMWSKDGMISKEAWATGSAVVREAGILKTDVKYEEIIDMSFVESVRSSL"
     gene            1369..>1964
                     /locus_tag="VM910_15910"
     CDS             1369..>1964
                     /locus_tag="VM910_15910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVI64049.1"
                     /translation="MSEPIWNKFLTERDKAVFKTSGYGARGGFGKRPALLIIDVNYAF
                     CDERPMPILESIKRWRNSCGEDAWVAMPYLKALIDKAHAKGIPVIYTTGVRREDNWDS
                     GSWTWKNGRSDEDMTSKPATNVDGNDIVAEIAPAPQDIVVLKQKPSGFFGTNMASYLT
                     LLGCDSVIVTGTTTSGCVRATVLDAFSLNYRIALAEEG"
BASE COUNT          399 a          629 c          599 g          337 t
ORIGIN      
        1 cacgcgcatg caggtgttcc gcaagctcgt cgtgccgtca tgcatgccat ggatcatctc
       61 ggtgctgcgg gtgaatatcg gcctcgccct gaccggcgcc atcgtcggcg agttcatcgc
      121 ctcccagcac ggcctcggcc gggccatcct ctatgccggc cagacctacg atatcgcgct
      181 ggtgtgggtg gcggtgctcg tactctcgac cttggccgtc gtgatgtatg cggcggtgtc
      241 gtggctggaa gcttggctgc gcaagggcgt gcggcaatga tcggattcgc gaccgcgaat
      301 gaaacagcga ggatgtcatg aacaggcgga aattcctgaa gtcgactgcg gcgctcggcc
      361 ttgcctcgat ggcgcagcca tccatccctc gtgccgccag ccgtgaaatc gtcgtcgccg
      421 agccggtcca cggcacgggc tatctgccac tctatatcgg catggccaac ggctatttcg
      481 ccgaggccga cgtgaccgtg cagattttga ccatcgagac cggcgccggt cacaccaatg
      541 cggtgctgtc cggacaggct ttcgccttca tcggcggccc cgaacacaac gctttcgcca
      601 aggccaaggg ggcggagctg cgcgccgtgg tccactgcgt cgaccgcggc aacgtgtact
      661 attgcgcggc caaagggcag gagcccgcca acagcgactg gcccggctat ttcaagggca
      721 agtcgatcgc gctgagccag ttcggcggca cgcccaattc catcacccgc tatctcttga
      781 agaagtggaa tctcgacccc aagagcgacg ttacgctcgt ggaagtcgcc aattccgccg
      841 tgctctccgt ggtgaaaagc aagcaggcgc agatcggcgt gtcgaccgag ccattcgtca
      901 cccagggcgt gcggcagtcg atctggtccg agccgttcta caatatcccc aagcaactgg
      961 ggccttacgc ctattccacc ctcaacgttc gcctcgatac catccaaaag gaacccgaat
     1021 tggtgcgcgc cttcgttcga ggtatggtca agggactcaa gttcacctat gcccatcccg
     1081 atcaggccgc cgaaatcgcc aagaagcaat tcccgaccat gccgctcgag gatctcaagg
     1141 cgacgctcga ccgctcgttc cgcgatgaga tgtggagcaa ggacggcatg atcagcaagg
     1201 aagcctgggc gaccggcagc gccgtggtgc gcgaggccgg catcctcaag accgacgtca
     1261 aatacgaaga gatcatcgac atgagcttcg tcgaaagtgt gcgctcaagc ctttgagcgt
     1321 caaaccgcgc caaattagac caagcgagaa accggaaaga gcaagaccat gtcagagccc
     1381 atctggaaca agttcctcac cgagcgcgac aaagcggtgt tcaagacgtc gggctacggc
     1441 gcacgcggcg gattcggcaa gcggccagcg ctgttgatca tcgacgtgaa ttacgcgttc
     1501 tgcgacgagc gccccatgcc gatcctcgaa tcgatcaagc gctggcgcaa ttcctgcggt
     1561 gaggatgctt gggttgccat gccctatctc aaggcgctga tcgacaaggc ccacgccaag
     1621 ggcatcccgg tgatctacac caccggggtg cggcgcgagg acaattggga cagcggcagc
     1681 tggacctgga agaacggccg ctccgacgag gacatgacca gcaagcccgc gaccaacgtg
     1741 gacggcaacg acatcgtcgc cgagatcgcg ccggcgccac aggacatcgt ggtgctgaaa
     1801 cagaagccgt ccggcttctt cggcaccaac atggcgagct atctcacgct gctgggctgc
     1861 gacagcgtga tcgtcaccgg cacgaccacg tcgggctgcg tgcgcgcgac cgtgctcgac
     1921 gccttcagcc tcaactaccg catcgcgctg gccgaggagg gctg
//
LOCUS       DATLJT010000693         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1880825,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000693 DATLJT010000000
VERSION     DATLJT010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880825"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_15915"
     CDS             complement(<1..230)
                     /locus_tag="VM910_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64050.1"
                     /translation="MGARISRSHWLALAGLVATLTIAVAGCDPAQARVKSKRDHARPR
                     AIHGANYHPPYADIVVDDKSGLVLHEVSADEPR"
     gene            complement(518..1570)
                     /locus_tag="VM910_15920"
     CDS             complement(518..1570)
                     /locus_tag="VM910_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI64051.1"
                     /translation="MLRLALRLVAAIGLPGLLAATALPARAQSQLYAEARAEGAFAFY
                     VGGPTAPWEAKAKIFEQRYPGIKVSITGGFSNVLDKKVDQQLKDDKLEVDAAIFQTLQ
                     DCVRWNAQGHLLSYKPQGFDAIDASFKDPGGAFYGVMVIAMPYMVNTEHVSSADVPDS
                     ALDFLKPQFRGKAVTPYPADDDATLWLFHHVVRKYGWDWMDKYMLNKPNFIQGHLGQQ
                     RSVASGQNIVAIDSIFNITEPMKQEGKPVASHFSTVDATPIWPLTGAIFKKAPHPNAA
                     RLFMGWLLEPEQQATTGTWSVRGDVPPPAGYKPIFSYKVVNDYRDFLTNEAQVVDLRK
                     RFEHYTGPVVNVGGVR"
     gene            1811..2497
                     /locus_tag="VM910_15925"
     CDS             1811..2497
                     /locus_tag="VM910_15925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI64052.1"
                     /translation="MRMIDLSRELYHRTPTYPGQRPIIHGVWKTHEESFVESGNVHGN
                     CVMFFSMPDHGGTHIDAPRHFGMNGTPIDEYPLENCYVKGICLDLRHIVPKAEITPSH
                     LEEAVRKSGSAIPKRGTVLLCTGHHARTFPTPAYSTDNPGVNVAATEWLARQGIVHFG
                     IDSMRPGPMGVVNSLVHKACLELDITHMESLCNLESLIGQGEFRFIGLPLKWRGGTGS
                     PIRAVAVFEE"
BASE COUNT          488 a          850 c          852 g          500 t
ORIGIN      
        1 cgcggttcgt cggcactgac ctcgtgcagc acgaggcccg atttgtcgtc gaccacgatg
       61 tcggcatagg gcggatgata attggcaccg tgaatggcgc gtggacgggc atggtcgcgc
      121 ttagacttga cacgcgcttg cgccggatcg cagccggcaa cggcaatcgt cagcgtggcc
      181 acgagaccgg caagcgcgag ccaatgcgag cgcgagatcc gagcccccat ttgcagcccc
      241 ctgtgatcga tcgcgaatcg tgtcgaccaa gtaaatcctg aattcggcgc gctggctgta
      301 atcggaatgc cacagttcgt cgaaatcgct cgcgataacc acgatggcgg tgctgcgggc
      361 gcggccgctc acgcgctagc gcgccttgcg cggggaaatg acatcagcgc gagcggcgaa
      421 ctcgggtccg acgcttctgc cgcgcgtttc cgcgcctgac agatggcgcc ctcatcttgc
      481 gccagcgggc acgccttgct tgcggggagg gcttcgccta tcgaaccccg ccaacgttga
      541 cgaccggacc ggtgtagtgc tcgaaccgct tgcgcaggtc gacgacctgc gcctcgttgg
      601 tcaggaagtc ccgatagtcg ttgaccacct tgtaggagaa gatcggcttg tagccggccg
      661 gcggaggcac gtcgccccgc acagaccagg tgccggtggt ggcctgctgc tccggctcca
      721 gcaaccaccc catgaacagc cgggctgcgt tcggatgtgg cgccttcttg aagatcgcgc
      781 cggtcagcgg ccagatcggc gtcgcatcca ccgtcgagaa atgtgaggcc accggcttgc
      841 cttcctgttt catcggttcg gtgatgttga agatcgagtc gatggcgacg atgttttggc
      901 cggaggcgac gctgcgctgc tggccgagat gcccctggat gaagttcggc ttgttgagca
      961 tgtacttgtc catccagtcc cagccgtatt tccgcaccac gtgatggaac agccagagcg
     1021 tggcgtcgtc gtccgccgga tagggcgtca ccgccttgcc gcggaattgc ggcttgagaa
     1081 aatcaagcgc ggagtccggc acatcggctg agctgacgtg ttcggtgttg accatgtacg
     1141 gcatggcaat gaccatgacg ccatagaacg cgccgcccgg atccttgaag cttgcgtcga
     1201 tggcatcgaa gccctgcggc ttgtagctga gaaggtggcc ttgcgcgttc cagcgcacgc
     1261 agtcctgcaa cgtctggaag atcgcagcgt caacctcgag cttgtcgtcc ttgagctgct
     1321 ggtcgacctt tttgtcgagc acgttggaga agccccccgt gatcgacacc ttgatgccgg
     1381 gataacgctg ctcgaagatt ttcgccttcg cctcccaggg cgcggtcggt cccccgacat
     1441 agaacgcgaa ggcaccttcg gccctggcct cggcgtagag ctgcgattgc gcacgagcgg
     1501 gtagggcggt tgcggcaagg agcccgggta ggccaatcgc cgccacgagg cgaagcgcaa
     1561 gacggagcat gagtaggtcc ccggcggccc gatgttgttg tggcggtaat catgcgggcc
     1621 aaagcagatg ccaacaagcc gcgcagcatg cagaagcctc atgggtgacc tgcgtcggcg
     1681 tacgtgccgc tgcgcgcgcg catgagcggc gctcgagcgg gctggttgcg ggcgtcgtcg
     1741 cttcaccttg aataatccgc tacaactcga tgcttttcgg ccgcaccgca aatcgaggga
     1801 ggaaaatgcc atgcgaatga tcgacctgag ccgcgaactc tatcatcgca cccccactta
     1861 tcccgggcag cggcccatca tccatggcgt ctggaaaact cacgaggagt ccttcgtcga
     1921 gtctggaaac gttcacggca actgcgtgat gtttttctcc atgcccgacc atggcggcac
     1981 ccacatcgat gctccccgcc acttcggcat gaacgggacg ccgatcgacg agtacccgct
     2041 tgagaactgc tatgtgaagg gaatttgcct cgatctgcgc cacatcgtgc cgaaggccga
     2101 aatcacgccg tcgcacctgg aggaggcggt gcgcaagtcg ggtagcgcca tcccgaaacg
     2161 cggcaccgtg ctgctctgca ccgggcatca tgcgcgcacc tttcccaccc ccgcctattc
     2221 gaccgacaat cccggcgtca acgtagcggc gaccgagtgg ctggcgcgcc aagggatcgt
     2281 gcatttcggc atcgactcca tgcgtcctgg acccatgggc gtcgtgaatt cgctcgtgca
     2341 caaggcgtgc ctcgagctcg acatcacgca catggaaagc ctgtgcaacc tcgagtcgct
     2401 gatcggtcaa ggcgaattcc gcttcatcgg cttgccgctc aaatggcgcg gcggcaccgg
     2461 ctcgccgatt cgcgcggtgg cggtgttcga ggagtaaagc cggacgcatc gaatgattgg
     2521 gcggctttgt cgctgcttga cccttccggc gcccggctct agcgtcaagc cgacgaaccc
     2581 gtggcctgga gggtcatgcg atggcgtcgt tcaccatcac accgctgacg gctcacaccg
     2641 gcgtcgaggt cgtcggcctc gacttcacaa agccgatcga caccgacgac
//
LOCUS       DATLJT010000694         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4408045,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000694 DATLJT010000000
VERSION     DATLJT010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4408045"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VM910_15930"
     CDS             <1..548
                     /locus_tag="VM910_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64053.1"
                     /translation="AIMATIAVVLEKETGSIANFRSTPISKFEFLAGKQFPYVAVGML
                     TFTLLVLMALFVFHVPVKGSFAALALGTLLYVFSTCGFGQLVSTFTQTQVAAVFATTV
                     LSVIPTVNFSGLLVPVSSLTGQGRLIGLMFPAAWFQPISVGTFTKGLGFSDLWLNALV
                     LAAFALVYLVAAQTVLQKQEA"
     gene            667..1761
                     /locus_tag="VM910_15935"
     CDS             667..1761
                     /locus_tag="VM910_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64054.1"
                     /translation="MIKELRSIRSDPIMLALVAYSFTIAIYAVATGASTEATNLSVGI
                     VDEDHSDLSRRIADGLTPPTFKPAVEIAATEIDASMNSERFIFVIEIPPKFQEDILAG
                     RQPSVQINVNATAVAQAFNGMTYIESVIVNYVTEFVSGREGLMGVPVKLVTRARFNPN
                     LKSSWFSSVMQIINNLTMLTVILTGAALIREREQGTVEHLLVMPVVPPEIMLSKILAN
                     GFVILAAAELSLLLVVQWWLQVPIAGSTLLFLGGSCFYVFTVGALGILLGTVAATMGQ
                     FGLLAIPVLLVMMLLSGGTTPMESMPVWLQYLMKVVSPTPHFVVFSQDVLYRGAGLSI
                     VWPEILATAVIGAVYFAFALRRFRSVIFGG"
BASE COUNT          355 a          637 c          587 g          452 t
ORIGIN      
        1 cggcgatcat ggcgaccatc gctgtcgtgc tcgagaagga aaccggttcg atcgccaatt
       61 tccgctccac gcccatcagc aaattcgagt tcctggcggg caagcagttc ccgtatgtcg
      121 ccgttggaat gctgactttt acccttctgg tgctgatggc gcttttcgtt ttccacgtgc
      181 cggtcaaggg ctctttcgca gccctcgcgc tcggcacgct gctctatgtt ttctcgactt
      241 gcggtttcgg acaactcgtc tcgaccttca cccagaccca ggtagcggca gtctttgcga
      301 ccaccgttct ctccgtcatt ccgacggtaa atttttccgg tttgctggtg cccgtgtcct
      361 cgctcactgg ccagggaagg cttatcggcc tgatgtttcc agcggcctgg ttccagccga
      421 tcagcgtcgg caccttcacc aaaggactcg ggttttccga tctctggtta aatgcgctcg
      481 tccttgcggc cttcgcgctc gtctacctcg ttgccgcgca gacagtgctg cagaagcagg
      541 aggcctgaga tgtcggtggc aactccgtcg gcaggggcgg cctcgcaagg ggtgaccaag
      601 acgaagcggc ccggaagcaa tcggctcgct aagctgcgcg tgcacctggc taacatctat
      661 cggctgatga tcaaggagct gcgcagcatc cgctccgacc cgatcatgct ggcgcttgtc
      721 gcctatagct tcaccatcgc catctacgcc gttgccaccg gcgcctcgac ggaagcgacc
      781 aatctttcag ttggaatcgt cgacgaagac cattccgacc tgtcgcgccg aattgcggac
      841 ggcctcactc caccgacatt caagccggcg gtggagatcg ccgcgacgga gatcgatgca
      901 tcgatgaatt cggagcgttt tatcttcgtc attgaaattc ccccgaagtt tcaggaggac
      961 atcctcgccg ggcggcagcc gtcggttcag atcaacgtca atgccacggc cgtggcccag
     1021 gccttcaatg gcatgaccta catcgagagc gtcatcgtca actacgtcac ggagttcgtc
     1081 agcggacgcg aggggctgat gggcgtgccg gtcaaactgg ttacgcgcgc aaggttcaac
     1141 ccgaacttga agtcgagctg gttctcctcg gtgatgcaga tcatcaacaa tctcaccatg
     1201 ctcaccgtga tcttgaccgg agcggcgctc atccgcgagc gtgagcaggg cacggtcgag
     1261 catctcctcg tgatgcctgt ggttccgccc gagatcatgt tgtcgaagat cctggccaat
     1321 ggcttcgtca ttctggccgc cgccgaactg tcgctgctgc ttgtggtgca gtggtggcta
     1381 caggtgccga tcgccggatc gaccctgctg tttctcggcg gctcctgctt ttatgtgttc
     1441 acggtcgggg cgctcggcat tcttctcggc acggtcgcgg ctacgatggg gcagttcgga
     1501 ctgctggcaa tcccggtgtt gctggtgatg atgctgcttt ccggcggcac cacgccgatg
     1561 gaaagtatgc cggtctggct gcagtacctc atgaaagtgg tcagcccgac gccgcatttc
     1621 gtcgttttct cccaggatgt gctctatcgc ggcgccggcc tctcaatcgt atggcctgag
     1681 attttggcca cggcggtcat cggcgctgtt tacttcgcgt tcgctttgcg ccgcttccgc
     1741 agcgtgatct ttggaggctg aacgaacgga gacggcgccc acgcgagtga acgcctagaa
     1801 ccgcatcgcc ataggattcc acgtggcgcc ccgccccgca acgtgagcaa cggcgccgct
     1861 gggtcggcgg atcgtcagcg acgtcgccgc gtaccaaccc gaaattcgat gacggcttca
     1921 gctgatccag ctcgccgccg cgagcacgac aattcccgtg acgaacgcgg ccatgcagga
     1981 atacagcaag atttcattga gaactttaac catcacactc tcccacacgc g
//
LOCUS       DATLJT010000695         1720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1880934,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000695 DATLJT010000000
VERSION     DATLJT010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880934"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VM910_15940"
     CDS             <1..567
                     /locus_tag="VM910_15940"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64055.1"
                     /translation="GLSQQSRELAGKRLELLREIVPALHRLAAMGNVGSPSFALEIGD
                     VQATVRALGLDVATFEIRRGEDIVPAFAAIKDRADALYVIGSDPLIVTKRVSISTLAA
                     AARLPTVHSTREYVEAGGLLSYGPNYADLFWRAGNYVDKILRGAKPGDLPVEQPTKFD
                     LVVNLTTAKALGLTIPESFLLRADEVIE"
     gene            564..1079
                     /locus_tag="VM910_15945"
     CDS             564..1079
                     /locus_tag="VM910_15945"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64056.1"
                     /translation="MRRREFITLLGGTVAAWPHAAVAQQAGKLPTIGFLGASTRSAEN
                     EWIAAFVQRLRELGWVEGRTIVIEYRWAEGHDERLAEIAAEFVRRKVDVIVTWGSEAA
                     VIAKQATSVIPIVFAAMGDPLGTGLVASLARPGGNITGLSNQSVELAGKRLELLREIV
                     PGLRRLAIMFV"
     gene            complement(1050..1382)
                     /locus_tag="VM910_15950"
     CDS             complement(1050..1382)
                     /locus_tag="VM910_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64057.1"
                     /translation="MAPSLVPAPSPIDRLVLDAGLTEAGFRRMGQAHSAGLDTVIEVD
                     QRYRQMFLLNRGYDVYVSDAVERGRASWARYPEFFKSEPLFRSKKEAWELFRFGPTGS
                     YEHDRQAA"
     gene            complement(1383..1550)
                     /locus_tag="VM910_15955"
     CDS             complement(1383..1550)
                     /locus_tag="VM910_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64058.1"
                     /translation="MNLCMALAAQCHQVTRKFIEDPQIGQVVNFRRGPFPAFLAGMVG
                     ALKDVPLGALA"
BASE COUNT          335 a          531 c          540 g          314 t
ORIGIN      
        1 ggattgtcgc agcagtcgcg ggagcttgcc ggcaagcggc tcgaactcct gcgcgagatt
       61 gtgcctgcgc tgcaccggct ggcggccatg ggcaacgtgg gcagcccctc ctttgcgctg
      121 gagatcggcg acgttcaggc gacggttcgc gcgctcggcc tcgacgtcgc cacctttgaa
      181 atccgccgcg gggaggacat cgtgccggcc ttcgcggcga tcaaggaccg cgccgatgcg
      241 ctttatgtca ttggcagcga cccgctcatc gtaaccaagc gggtgtccat cagcacactt
      301 gcggccgcag cgcgcttgcc gaccgtgcac agcacacggg aatacgtcga agccggaggt
      361 ctgctgtcct atggtccaaa ctatgcggac ctgttctggc gcgcaggcaa ctatgtcgac
      421 aagattctac gcggcgcgaa accgggggac cttccggtcg agcagccgac caaattcgat
      481 ctcgtggtca acctgaccac cgccaaggcg ctcggcctca cgatccccga atcgttcctg
      541 ctgcgcgccg acgaggtaat cgaatgaggc gacgcgagtt catcacgctg ctcggcggca
      601 cggtagcagc gtggccgcac gcggcggtcg cgcagcaggc gggcaagctg cccaccatcg
      661 ggttcttggg agcaagcacg cgttcggccg aaaacgaatg gattgccgcg ttcgtgcagc
      721 ggctgcgcga gcttggttgg gtcgagggcc gcaccatcgt gatcgaatat cggtgggccg
      781 agggacacga cgagcgtttg gccgagattg cggccgagtt cgtccggcgc aaagtcgatg
      841 tcattgtcac gtggggatcc gaagccgccg tcatagcaaa gcaggcgacg tcggtaatcc
      901 cgatcgtctt tgcggcgatg ggggacccgc ttggcaccgg cctcgtcgcc agtctggcgc
      961 ggccgggggg caatatcact gggctatcca atcaatcggt tgaacttgcg ggcaagcgtc
     1021 ttgaactgtt gcgcgagatt gtccccggtc tacgccgctt ggcgatcatg ttcgtatgat
     1081 ccggtgggcc caaaacggaa cagctcccac gcctcctttt tggagcggaa gagtggttcg
     1141 ctcttgaaga actcgggata gcgcgcccag gatgcccggc cgcgctcgac agcgtcagac
     1201 acgtacacgt cataaccgcg attcaataaa aacatctgcc ggtatcgctg atcaacttca
     1261 attaccgtgt cgagccccgc ggaatgcgcc tgccccatgc gccgaaatcc ggcttcggtc
     1321 agtcccgcgt cgaggacaag ccgatcaata ggagaaggcg caggaaccag ggatggcgcc
     1381 aatcaggcga gcgccccgag cggcacatcc ttcagcgcgc caaccatacc tgcgagaaat
     1441 gcgggaaacg gcccgcgacg gaagttcacc acctgaccta tctgcgggtc ttcaatgaat
     1501 ttccgagtga cttggtggca ttgtgccgcc aatgccatgc agagattcat tggcgacagc
     1561 cagcacttgg gtcgaggcta cccgtgcccg gtcgcatatt cgatcacatt agcgcgaccc
     1621 ttgccacgac tccacgtaac ggaagcgacg agacacattt catcgcgcgg ggttcgattg
     1681 gatcatcggt cagctcccat ttagccgaaa gtgaatgcgt
//
LOCUS       DATLJT010000696         1531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2800582,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000696 DATLJT010000000
VERSION     DATLJT010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800582"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VM910_15960"
     CDS             <1..929
                     /locus_tag="VM910_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64059.1"
                     /translation="LDWKYEGTQAPFLLALDRGYFKAEGLDVTIDTAGGSVEPINRIA
                     SRSYDMAFADINSLIKFRDQNPQTPLKAIFMIYSNPPFAVVTRKSRGIASPKDLEGKK
                     LGAPAADGAYAQWPIFVKANGIDASKVEILNVGFAVREPMLVSGHVDAITGFSFSTYV
                     NVKHSGVAQDDIVVLLMANYGVRLYGNTIMVNPSFAADKPQAVKGFLRGFLKALKDTT
                     KEPAAAIDSVLKRNETANREIELERLQIALRDNILTPEVKANGFGDVDLKRLDTAIDQ
                     IALTYTFKSAMPKADDVFDPSFLPPAAERKAD"
     gene            1165..1497
                     /locus_tag="VM910_15965"
     CDS             1165..1497
                     /locus_tag="VM910_15965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI64060.1"
                     /translation="MVARRRTQRAAVFRAIADPTRREILSLLRRGPHTVGAIAANFRT
                     SRPAISKHLRLLRSVGLVATRQDGTARVCELNARPLRAVSEWLRDYEAFWGESLRSLK
                     RYVEEDGR"
BASE COUNT          307 a          492 c          453 g          279 t
ORIGIN      
        1 cgctcgattg gaaatatgaa ggcacgcagg cgcccttcct gctggcgctc gaccgcggct
       61 acttcaaggc cgaaggcctc gacgtgacca tcgataccgc gggcggctcg gtcgagccga
      121 tcaaccgcat cgcctcgcgc agctacgaca tggcattcgc cgacatcaat tcgctgatca
      181 agttccgcga ccagaacccg cagacgccgc tcaaggcgat cttcatgatc tacagcaacc
      241 cgccgttcgc ggtcgtcacg cgcaagagcc gcggcatcgc ctcgcccaag gacctcgaag
      301 gcaagaaact cggcgcgccg gccgcggacg gcgcctatgc gcaatggccg attttcgtga
      361 aggcgaacgg catcgatgcc tccaaggttg agatcttgaa cgtcggcttc gcggtgcgcg
      421 agccgatgct ggtgtcgggc cacgtcgatg ccattaccgg gttttcgttc tccacttacg
      481 tcaacgtcaa gcacagcggc gtcgcgcaag acgatatcgt cgtcctcttg atggcgaatt
      541 acggcgtcag gctctacggc aacacgatca tggtcaatcc gtcgttcgcc gccgacaagc
      601 cgcaggcggt caagggattt ctccgcggct ttctgaaagc gctcaaggac accacgaaag
      661 agcctgcggc cgccatcgac tcggtgctca aacgcaacga gaccgccaac agggagatcg
      721 agctggagcg gttacagatc gcgctgcgcg acaacatcct tacgccggag gtcaaggcga
      781 acgggttcgg cgacgtcgac ttgaagcggc tcgacaccgc gatcgatcag atcgcgctga
      841 cctacacctt caagtcggcg atgccgaagg ccgacgacgt cttcgaccca tcgtttctgc
      901 cgccggcggc cgagcgcaag gccgactgac gcagctgcag tcgcacagct ccggacgcga
      961 agcggttcgc ttttcgcgcc catcatacga acgtctttcg catcgacttc gacgcgctgc
     1021 cccaacccgc gaaagtcgcg ttgaaatgcg gccgttttca cccgcccgcc ttgtcaaaac
     1081 gcaatctttc ggccttcgac ggccttgacc aatccgtaac cttttggtta cagtattggt
     1141 aaccaaatgg tcacgttacg ttaaatggtc gcacgcagac gcacgcaacg cgcagcggtg
     1201 tttcgggcaa ttgccgaccc cacccgtcgc gagattctga gcctgctgcg ccggggtccc
     1261 cacaccgtcg gcgcgatcgc ggccaatttt cgcaccagcc ggccggcaat ttcgaagcac
     1321 cttcgcttgc tgcgatccgt gggtctggtc gctaccaggc aggacggaac ggcgcgtgtt
     1381 tgcgaattga atgcgaggcc cctgcgtgcg gtcagcgagt ggctccggga ctacgaagcg
     1441 ttctggggcg agagtttgcg cagtctcaag cgttacgttg aggaggacgg tagatgaatc
     1501 tgacagacgc acgcgacacg atcgtcaagg a
//
LOCUS       DATLJT010000697         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1880990,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000697 DATLJT010000000
VERSION     DATLJT010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880990"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(470..1678)
                     /locus_tag="VM910_15970"
     CDS             complement(470..1678)
                     /locus_tag="VM910_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HVI64061.1"
                     /translation="MSRPSLEVADVFRDHGPAWRIANAGHVSLGQLKVMSAIESCRTA
                     TLGGHVERCKACGHTRIAYNSCRNRHCPKCQGAAAKEWLADREAELLPVPYYHLVFTL
                     PAPIADIAYYNKAVIYGILFKTAAETLLTIAADPQHLGARIGLTAVLHSWGSALTHHP
                     HVHIIVPGGGISLDGQRWISCRPGFFLPVRVLSRLFRRLFLAKLIAAYGTGHLQFFGD
                     HARLVERRAFTAYLAPLRRAEWVVYSKRPFGGAEAVLAYLSRYTHRVAISNSRLIGIT
                     RTSVTFKWKDYRANGHDRAKVMTLAIDEFIRRFLIHVLPDGFHRIRHYGLFANGGRVE
                     NIARARQLLNLPTPQNGPSDGHSTSDGEQQTLAQPCPCCGGRMIIIETFERGCGPRYR
                     PAVPTRIDSS"
     gene            complement(1691..>2182)
                     /locus_tag="VM910_15975"
     CDS             complement(1691..>2182)
                     /locus_tag="VM910_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI64062.1"
                     /translation="AALSVAYGAGLRASEVVSLKIEDIDSTRMVIRVEQGKGRKDRYV
                     MLSPYLLDLLRAWWRAARPRGWLFPGRDPIQPLTTRQLNRACHAAAMMAEIDKPVSLH
                     TLRHSFATHLLEQKTDIRVIQVLLGHKKLDTTALYTRVALKALGEITSPLEQLVTTMR
                     PPA"
BASE COUNT          390 a          634 c          785 g          373 t
ORIGIN      
        1 catttgacct atgcacacaa tgaaatctcc gtgaacggaa cgcaggtcac gacacatcga
       61 ttcccgcgag cggttgaatt tagcacctga ggatattccg cttttgttaa gggttatcag
      121 atgcctgccc ggcacaggac agtgcggcgg gctctgaggg caggcgtctg ataagcctca
      181 ggggaggaaa ccgcgggccg gcatttgtag gacggtgctg cgccagagct atggggattt
      241 gagggccccg ggcagtgggc tgaggcgcga gtatcgtaag caccggagcg gcgcggctgc
      301 acgcgatcgc agtgacggag cgacccggtg cagtgaacga gttgcaattg gtaacgtcga
      361 aggcgcgagc gggcgcacgg ctctggtcat tggccgaatg ggcccgcggc cggtcgacga
      421 caagcggcac tgcgagcagg attgaagcgt tgcgatctgg cgacggcgat catgagctgt
      481 cgatgcgagt aggaactgcc gggcgatacc gcggtccaca accgcgctcg aaggtctcga
      541 tgatgatcat gcggccgcca cagcagggac agggctgcgc gagtgtctgc tgctcaccgt
      601 cactggtgct gtggccatca ctgggcccat tctgcggcgt cggcaggttg aggagctgac
      661 gcgctcgcgc gatattctcg acgcgcccgc cattggcgaa gagaccgtag tgacggatgc
      721 ggtggaaacc gtcgggcagg acgtggatca ggaagcggcg gatgaactcg tcgatggcga
      781 gcgtcatcac cttggctcgg tcatgcccgt tggcgcgata gtccttccac ttgaaggtca
      841 cgctggtacg ggtgatgccg atcagacggc tgttggagat cgcgacgcga tgcgtgtagc
      901 gcgagagata ggccagcacc gcttcggctc cgccgaacgg gcgcttgctg tagacgaccc
      961 attcggctcg acgcaaaggt gccagatacg cggtgaaggc ccggcgctcg acgaggcgcg
     1021 cgtgatcgcc gaagaactgc aggtggccgg tgccgtaggc ggcgatcagc tttgccagga
     1081 acaggcgtcg gaagagccgc gacagcacgc gcaccgggag gaagaagccg gggcggcacg
     1141 agatccatcg ttgcccatcc agcgagatgc cgccgcccgg tacgatgata tggacgtgcg
     1201 ggtggtgggt gagcgccgag ccccagctgt gcagcaccgc ggtgaggccg atccgggcgc
     1261 cgaggtgctg cgggtcggcg gcgattgtga gcagcgtctc ggccgcggtc ttgaacagga
     1321 tgccgtagat cacggccttg ttgtagtagg caatgtcggc gatcggcgcc ggcagcgtaa
     1381 acacgaggtg gtagtagggc accgggagca gctcggcctc gcggtcagcc agccattcct
     1441 tggctgccgc accctggcac ttcgggcagt ggcgattgcg gcatgagttg taggcaatgc
     1501 gcgtgtggcc acatgccttg cagcgctcga catgcccgcc gagcgtcgcc gtccggcagc
     1561 tctcgatggc tgacatgacc ttgagttggc cgaggctcac atggccggca ttggcgatgc
     1621 gccacgccgg tccgtgatcg cggaagacgt ccgcaacctc cagcgaagga cgggacaagt
     1681 gcgccgcggt ttacgcgggc ggcctcatcg tcgtgacgag ctgttcgagc gggctggtga
     1741 tctcgccgag cgccttgagg gcgacgcggg tatatagcgc ggtcgtatcg agcttcttgt
     1801 gcccgagcag cacctgaatc acgcgaatgt cggtcttttg ctcgagcaga tgggtggcga
     1861 agctgtgccg cagcgtgtgc agcgagacgg gcttgtcgat ctccgccatc atcgccgcgg
     1921 cgtggcaggc gcggttgagc tggcgcgtcg tcagcggctg gatcggatcg cggccgggaa
     1981 acagccaacc ccggggccgc gcggccctcc accaggcccg taacaggtcg aggaggtacg
     2041 gtgacagcat cacgtagcga tccttgcggc ctttgccttg ctcgacccgg atgaccatcc
     2101 gggtgctgtc gatgtcctcg atcttaagcg agacgacttc ggaggcacgc aatcccgcgc
     2161 cgtaggcgac actcagcgcc gc
//
LOCUS       DATLJT010000698         5132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3259717,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000698 DATLJT010000000
VERSION     DATLJT010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3259717"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VM910_15980"
     CDS             complement(<1..948)
                     /locus_tag="VM910_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64063.1"
                     /translation="MTQGIGARIQRKEDARHLHGRGNFVSDMILPGQSEVAFLRSPIA
                     HGRVRRIVKPPGTENQIFIRADLGDVAAMVAPTTVPGYKLSEWHPLAHEKVRFVGEAI
                     AMCVAPTRAEAEDLGEQIEVEFDELPVLVEAQAARMESAVRVHEQWNDNVFLTLNYES
                     GFEVKAKSAAVVIQREIALARQAMVPMEGKAVLAHWDDRSDQLVVYASTQVPHIIRVG
                     LAQFLGIDQGRVRVISPDVGGGFGYKCILQPEELCVAWLALKFRRPFRYVEDRREHLV
                     VGANSRQHHYRLTAHADERGRLLALDADITIDGGAYSAWP"
     gene            complement(1015..2106)
                     /gene="dctP"
                     /locus_tag="VM910_15985"
     CDS             complement(1015..2106)
                     /gene="dctP"
                     /locus_tag="VM910_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HVI64064.1"
                     /translation="MQRRAFLMRAGAGVAATAVARPAIAQTGTPVRWRLATSWPKSLD
                     TLYGSVEAMCQRVGQLTDRKFQIQSFAGGEIVPPLQVWDATQNGTVECGHTLTSFNIG
                     KNPAVAFDSGLAFGLNTRQQQAWMNYGGGLELIRTLFKKDGILPIPCGNVGVQMGGFY
                     RKEINSVDDLKGLKFRIGGLGGMILAKLGVVPLQIPTADIYPSLERGAIDAAEWIGPY
                     DDEKLGLHKVAKYYYYPGWWEGSAQVTLLVNLQHWEALPESYRAALEAACAEATVLMM
                     AKYDTRNPEALRRLVAAGVQLRQFPRPVLDACYKAAFETYDELAGRSADFKLIYESWQ
                     KFLADSNLWFRVAENTLDNYRFAMSAQPR"
     gene            2388..2864
                     /locus_tag="VM910_15990"
     CDS             2388..2864
                     /locus_tag="VM910_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI64065.1"
                     /translation="MRIGTVHSEMPLWGRPGYLLRRLHQIHYALFFEECAGFDITPVQ
                     YGLLTTLSLNPNLDQNSLARELGIDRTNVADVLNRLARRGLLERRRSADDGRMVLARL
                     TRAGERLTRKMYRPMRRAQKRLLEPLLAQERNALIITLIRLINGNNHLGRTIFQPS"
     gene            complement(2919..3629)
                     /gene="maiA"
                     /locus_tag="VM910_15995"
     CDS             complement(2919..3629)
                     /gene="maiA"
                     /locus_tag="VM910_15995"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVI64066.1"
                     /translation="MSRWSRRRPTRFSTKPLRQSRRLSRDLYTFFRSSTSYRLRIALA
                     YKRLEYTPHYVSLPKMEHRTPSYRALHAQGLVPLLIDDDQSFMQSMAIIEYLDEVYPE
                     PPLMPADVRGRHYVRAVSQIIGCDIHPLNNVRVLKHIHTQFGADEAATKTWYRHWIAE
                     GLGGLETYVAGAGFARRFCYGDTVTMADICLVPQIFNAQRFDCPLDDYGTLTAIFERC
                     MALDAFRATQPNTQADAF"
     gene            complement(3568..3942)
                     /locus_tag="VM910_16000"
     CDS             complement(3568..3942)
                     /locus_tag="VM910_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64067.1"
                     /translation="MAQEPLATKGNFLHIYDFRVTKGKEEEFVRSFETFDYSDGNPMH
                     KSWAQVKDGVLCRDTQDPQRFFLIAEWSDIEEHARIRKVLANEIKPEFIKYIEGGTFI
                     PKYVEVVSSTPDEILNKAAAAE"
     gene            4229..>5132
                     /locus_tag="VM910_16005"
     CDS             4229..>5132
                     /locus_tag="VM910_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64068.1"
                     /translation="MRTHSPILCIAVAASVALFAAANAAHAQDKVIRIGGLFAMSGPG
                     SYFGVQDKQGIELAIEQLNKTGVNGYRFEVQYEDSACSPLPATQAAKRLLEQYKPQVV
                     LGEECSDATLAIMPLMEQAKVPLLNAGSSSIKITDPGNPWTFRIMPNEVMQGVDIATH
                     AYKRLNARTAVLLYENTNAGIGNAKVFGDTFKSLGGQILADIGFGRDVNDFTAIATRI
                     AGLGKVDVIPTYTLEGQGLRITQALAQAGVTKGGGGQSIQLGTIWLPFGFEQKAGKAA
                     LGYVRIVQFDPTDKRDVVQNFIRSF"
BASE COUNT         1005 a         1560 c         1566 g         1001 t
ORIGIN      
        1 cggccacgcc gagtacgcgc cgccgtcgat cgtgatgtcc gcatcgagcg ccaacaacct
       61 gccgcgttcg tcggcatggg cggtcagccg gtagtgatgt tgtcgtgagt tggcgccgac
      121 caccagatgc tcgcggcgat cttcgacgta gcggaacggc cgtcggaatt tcagcgccag
      181 ccacgccacg cacagctctt ccggctgcag gatgcatttg tagccaaagc cgccgccgac
      241 gtccggcgaa atcactcgca cgcgcccttg gtcgatgccg agaaactgcg ccaagccgac
      301 acggataatg tgcgggactt gggtcgaggc atagacgacg agctggtcgc tgcgatcgtc
      361 ccagtgggcg agcacggctt tgccttccat cggcaccatg gcctgacgcg caagcgctat
      421 ctcccgctgg atcacgaccg ctgcactttt ggccttcacc tcgaaaccgc tctcgtagtt
      481 gagcgtgaga aagacgttgt cgttccattg ctcgtggacg cggaccgccg actccatcct
      541 cgccgcctgc gcctcgacga ggaccggcag ctcgtcgaat tcgacctcga tctgttcgcc
      601 gagatcctcg gcctcggccc gcgtcggggc gacgcacatc gcgatcgcct cgcccacgaa
      661 gcggactttc tcgtgcgcca acgggtgcca ttcggataat ttgtagcccg gcacggttgt
      721 cggtgcgacc atcgctgcga catcgcctag atccgcgcgg atgaaaatct ggttttcggt
      781 gccgggcggt ttgacgatgc gccggacgcg gccatgcgcg atggggctgc ggagaaaggc
      841 aacctcgctc tgccccggaa ggatcatatc cgagacgaag ttgccgcggc cgtgcaggtg
      901 gcgggcgtcc tccttgcgct ggatccgggc tccgatccct tgcgtcatgc ttgatctcgg
      961 tgcaaacgcg gccgctgcca actcacggtc tgtcgcgcgg gcgctgtggt ggcgctaccg
     1021 cggctgtgcg gacatggcga agcgatagtt gtcgagcgtg ttctcggcga cccggaacca
     1081 aagattggag tccgccagga acttctgcca cgactcgtaa atcagcttga aatccgcgct
     1141 cctgccagcg agctcgtcgt aggtttcgaa cgccgctttg taacatgcgt ccaaaaccgg
     1201 acgtggaaat tgccgcagct ggacgcctgc cgcgacgagc cggcgcaacg cctcggggtt
     1261 tctggtgtcg tacttggcca tcatcaggac ggttgcttcg gcgcacgccg cctccagcgc
     1321 ggctcgatag gactccggca aagcctccca atgctggaga ttgacgagca atgtgacctg
     1381 agcgctgcct tcccaccagc cagggtagta gtagtacttg gcgaccttgt gcaggccgag
     1441 cttctcgtcg tcgtaaggac cgatccactc ggcggcatcg atcgcgccgc gttcgaggga
     1501 aggataaatg tcggccgtgg gaatctgcag gggcacaacg ccgagcttcg ccaggatcat
     1561 gccacctagt ccgccgatgc ggaacttgag ccctttgagg tcgtcgacgg agttgatctc
     1621 cttacggtag aagccaccca tctgcacgcc cacattgccg cagggaatgg gcaggattcc
     1681 gtccttcttg aacaacgtgc ggatgagctc cagccccccg ccgtaattca tccaggcctg
     1741 ctgctggcgc gtgttgagcc cgaacgcgag cccggaatcg aacgcgaccg cggggttctt
     1801 accgatattg aacgaggtga gcgtgtggcc gcactcgacg gtgccgtttt gcgtcgcatc
     1861 ccagacttgc agcggcggca cgatttcccc gccagcgaag ctctggatct ggaacttcct
     1921 gtcggtcagt tgccccacgc gttgacacat cgcttcgaca ctgccgtaaa gcgtgtcgag
     1981 gctcttcggc cagctcgttg ccagccgcca acgtactggg gtgccagtct gagcgatcgc
     2041 cggcctggca accgcggtcg cagccacgcc cgcgcccgct cgcatgagaa acgcacgcct
     2101 ttgcatttgg ctctcctgct gagatgtaac tgcccgaacc tgcaccgccg tcggatttgt
     2161 agtttttttg ccgattgcat ctgcgcgcag cgtgggtaaa agcctaacac agtcccggac
     2221 gcgtgcaagt atgactgtat actgaatatt tgccgctgcg cgcgaagcgg gactggtgcg
     2281 acgaggagga gagtccaatg gcgacaagaa tgaaaacggc aagaaaacac gtagagagac
     2341 tcgccggcga aaggaaacct cggcgacgca aggccgagaa aagatcattg cgcattggga
     2401 cggtccattc cgagatgccg ctctgggggc ggcccggcta tcttctgcgg cgcttgcatc
     2461 aaatccacta cgcgctgttt ttcgaggaat gcgcgggatt cgacatcaca ccggttcagt
     2521 acggattgtt gacgacgctc tcgctcaacc ccaatctcga ccaaaattca ttggcccgcg
     2581 aactcggaat cgaccgcacc aatgtcgccg atgtcctcaa ccgcctcgcg cgacgcgggc
     2641 tcctggagcg ccgccgcagc gctgacgacg gacgcatggt ccttgcgcga ttgacccgcg
     2701 ccggcgagcg actgactcga aagatgtatc gaccgatgcg acgcgcccag aaacgtctac
     2761 tcgagccgct tctcgcgcag gagcgtaacg cgcttatcat aacgctgatc cgcctcatca
     2821 acggcaacaa tcatcttggg cgaacgatct ttcagcccag ttgaggtcga tgaccgagaa
     2881 cgggtgggtc ttgccgatgc ccgctcacgc cgtccggttc agaatgcgtc ggcttgggtg
     2941 ttaggctgcg tcgcgcgaaa ggcgtcgagg gccatgcatc gctcgaagat cgcagtcagg
     3001 gtgccgtagt cgtcgagcgg acaatcgaag cgctgggcat tgaaaatctg cggcaccaga
     3061 caaatgtcgg ccatggtgac ggtatcgcca tagcagaagc gacgggcgaa gcccgcgcca
     3121 gccacgtaag tttccagacc tcccagtccc tccgcgatcc aatggcgata ccacgtcttg
     3181 gtcgctgcct cgtcggcacc aaattgcgtg tggatgtgct tgagcacgcg tacgttgttg
     3241 agaggatgaa tatcgcaacc gatgatttgt gacaccgccc gcacgtagtg gcgcccacgc
     3301 acgtctgccg gcatcagcgg cggttccggg taaacctcgt ccagatactc gatgatggcc
     3361 atggattgca tgaaggattg atcgtcatcg atcagcaagg gcactaaccc ttgggcatgc
     3421 agcgcccgat atgagggcgt tcgatgctcc atttttggca ggcttacgta atgcggcgtg
     3481 tattcgagcc gcttgtaggc cagcgcaata cgaagccggt atgaggtgga agaccggaag
     3541 aacgtgtata gatcgcgcga caaacgtcta ctctgccgca gcggctttgt tgagaatctc
     3601 gtcgggcgtc gacgagacca cctcgacata tttgggaatg aatgtccctc cttcgatata
     3661 tttgatgaat tccggcttga tctcgttggc cagcaccttg cggatgcgcg cgtgctcttc
     3721 gatgtcggac cactccgcga tcaaaaagaa acgttgcgga tcctgggtgt cacggcacag
     3781 cacgccgtcc ttcacttgcg cccacgactt atgcatgggg ttgccgtccg aatagtcgaa
     3841 cgtctcgaac gagcggacga attcctcctc cttgcccttg gtcacgcgaa aatcatagat
     3901 gtgcagaaag ttgcccttcg ttgcgagggg ttcttgcgcc atggcgtcct cctgttcctg
     3961 atcgatacga aggtacgctg atgaattttt gaaagcaacc acggctggat gtagccggag
     4021 atcatcgtgc catagacata tgattggcga tgatcgccgg catatcgatg ccattgacga
     4081 gtggcgcacc gccgagcgcc agtgcgggta gggcgccgcc gataggcggc gaagttgtcg
     4141 cgtggcttct tgctcggcga atgataaaag attggcaggc gtattggttt cgggcttaaa
     4201 tgaaacgata gccgcgaggg agagggccat gagaacacat tcccccattc tgtgtatcgc
     4261 tgtcgctgca agtgttgctc tgttcgctgc tgcgaatgcc gcccacgcgc aagacaaggt
     4321 gatcaggatc gggggcttgt tcgcgatgtc gggccccggt tcctatttcg gggtccagga
     4381 caagcagggc atcgagctcg cgatcgagca gctcaacaag accggggtca acggctacag
     4441 gttcgaggtc caatacgagg attcggcatg cagcccgcta ccggcgacgc aggcagccaa
     4501 gcggctcctg gagcaataca agccccaagt cgtgctcggt gaagagtgtt cggacgcgac
     4561 gctggcaatc atgccgctca tggaacaggc gaaggtgccg ctgctcaacg cgggctcgtc
     4621 ctcgatcaaa atcaccgacc ccggcaaccc gtggacgttt cgcatcatgc ccaacgaggt
     4681 gatgcagggc gtcgacatcg ccacccatgc ctataagcgc cttaacgcgc gcacggcggt
     4741 actgctctat gagaacacca atgccgggat cggcaacgcg aaggtgttcg gcgacacgtt
     4801 caagtcgctc ggcgggcaaa tcctggccga tatcggcttc ggccgcgacg tgaacgactt
     4861 caccgcgatc gcgacgcgca tcgccggtct cggcaaagtg gatgtgatcc cgacctatac
     4921 gctcgagggc cagggtctac gcatcacgca agcgctggcg caggctggtg tcaccaaggg
     4981 cggcggcggg caatcgatcc agctcggcac gatctggctg cccttcggtt tcgagcagaa
     5041 ggcgggcaag gcggcgctcg gctacgtccg catcgtccaa ttcgatccca ccgacaagcg
     5101 cgacgtggtg cagaacttca ttcgctcctt ca
//
LOCUS       DATLJT010000699         3738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4179164,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000699 DATLJT010000000
VERSION     DATLJT010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179164"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VM910_16010"
     CDS             <1..672
                     /locus_tag="VM910_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI64069.1"
                     /translation="KEEDIKATFLTCGAIAERFPEAVQAILDHGHVVEGHGYNHEIAR
                     YLPRDEEDQVMKKTIAMVHKRTGRRCYGWRSCTQSTNTIELLIENGFVFNTNCFHEEL
                     PFLWEKDGKLLVELPRQPFGDGTLFGHRHGEYGNPYHGLETWKAYFDELYSESSAERP
                     KYIPFTMHPYIIGRPGRTLALRGIIHHIKRAGDLWFPTGIELAEWCLNDVFQAECQKL
                     RASTG"
     gene            864..1886
                     /locus_tag="VM910_16015"
     CDS             864..1886
                     /locus_tag="VM910_16015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64070.1"
                     /translation="MGTATTTAVAVGLAAALLASAPTQAQPVSDFYRGKTLRMLIGYG
                     PGGGYDIYGRLVAEFLPRHLPGTPTMVTQNMPGAGSFVAAKYMYDVAPQDGTVFGSLA
                     QTLALDSMTNTNAKLDVGKMPYIGRVVTNIDVGAALPKTGIESFQDVRAKQYSVGASG
                     GGSTTVLFPTALKIYAGAKFKLVRGYSGTSDILLAMERGEVDIVGAYGLPGILVSHPG
                     WVHSGEATILYQAAVKRHRLLPNVPSLLELAQSNEGRQVLSAAASTGEIGRSILTTPG
                     VPPERLAALRSAFQAMLKDPDFVSACEKRNLMVDAAAGEEIDEIVRETLRLPQALAET
                     IGQMMQ"
     gene            2754..>3738
                     /locus_tag="VM910_16020"
     CDS             2754..>3738
                     /locus_tag="VM910_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HVI64071.1"
                     /translation="MMMLAPVLTPHGLLMLAQSLEAEEEALALEPERGSRLEKAFARG
                     AGHGLLLLGADEVGTALSPTLSYWRKLGARYMTALCALPGIGERSKPPVPIPADGELD
                     SMAAAVPPMIGAEYVTTEVLVNLWRGMDSAFDTELAQSKLTVQEFLKRRHPAWNLVGR
                     VHFNLAENRKDEDAPFAFLATYTTRLSAEARPQHLPLGKALQEYSGARNRERLLSLLM
                     PVQRAAEHCPWLKAMVDAGEIFHPLRWSPQQAVEFLRDVPALESAGVVVRMPASWRMN
                     RPARPQVMATVGGNAPSHLGMDALLDFRMEVTLDGENLTAVEIKRLLAHSDG"
BASE COUNT          722 a         1127 c         1177 g          712 t
ORIGIN      
        1 aaggaagagg acatcaaggc cactttcctg acctgcgggg caatcgcgga gcgcttccca
       61 gaggccgtgc aggccatcct cgatcacggc catgtcgtcg agggccacgg ctacaatcac
      121 gagatcgcgc gctacctgcc gcgcgacgag gaagatcagg tgatgaagaa gaccatcgcc
      181 atggtccaca agcgcacggg gcggcgttgc tatggctggc gctcctgcac ccagagcacg
      241 aacaccatcg agctcctgat cgagaacggc ttcgtgttca acaccaactg cttccacgag
      301 gagctgccgt tcctctggga gaaggatggc aagctgctgg tcgagctgcc gcgccagccg
      361 ttcggtgacg gcacgctgtt cggccaccgc cacggcgaat acggaaatcc gtatcacggg
      421 ctggagacct ggaaggccta tttcgacgag ctctactccg agagcagcgc ggagcgtccg
      481 aaatacattc cattcaccat gcatccctac atcatcggca gacccggccg tacgctggcg
      541 ttgcgcggca tcatccatca cataaagagg gccggagatc tctggttccc taccggcatc
      601 gaactcgccg aatggtgcct caacgacgtg tttcaggccg aatgccagaa gttgcgcgcg
      661 tccaccggat gacgcgaatg ccagcgcagt gttgcgccga cctcagatga ctgcggcggc
      721 gcccggcatc ccgatgcgcc ggtccaccgt tttgcacctt cgtgacaatt tcaccaagcc
      781 cgccgcacga gatcacgcta tcttggccgc agcgtctcaa taggaccggc gcagcgccat
      841 caaaccggcg atgggggaaa gtgatgggca cggccacgac gactgccgtt gcggttgggc
      901 ttgcagcggc cctgttggcg agtgcgccga cccaagctca acccgtctcg gatttctatc
      961 gcggcaagac cctgcgcatg ctgatcggct acggcccggg cggcggctac gacatctacg
     1021 gccggcttgt tgccgagttt ctgccgcggc atctgcccgg cactccgaca atggtcacgc
     1081 agaacatgcc aggggcgggg agcttcgtcg cagcaaaata tatgtacgac gtggcgcccc
     1141 aggacggcac ggtgttcggc agccttgccc agacgctggc gctcgacagc atgacaaaca
     1201 ccaatgcgaa gctcgatgtg ggaaagatgc cgtatatcgg ccgcgttgtg accaacattg
     1261 acgtcggtgc tgcgctgcca aagaccggca ttgaatcgtt ccaagacgtc cgcgcgaaac
     1321 aatatagtgt cggcgcatcc ggcggcggct ccactacagt tctcttcccc accgcgctca
     1381 aaatttatgc cggagcgaag ttcaagcttg tccgcgggta tagcggcacg agcgatatct
     1441 tgcttgcgat ggaaaggggt gaggtcgata tcgtcggcgc ctatggcctt ccaggcattc
     1501 tggtgagcca tcccggttgg gtgcacagcg gtgaagcgac aatcctttac caagctgccg
     1561 ttaagcggca tcgccttcta ccgaacgtgc caagtcttct ggagcttgcg cagtctaacg
     1621 agggccgtca ggtgctgagc gctgcggcca gtaccggcga aatcggtcgc tccattctca
     1681 cgacgccggg cgtgccgccg gagaggcttg cggcgctgcg atccgcgttt caagcgatgc
     1741 tcaaggatcc ggatttcgtc tcggcctgcg agaaacgaaa cctgatggtt gatgctgccg
     1801 ccggagaaga gatagatgaa attgtccgtg agaccctgcg gttgccgcag gcgctggcgg
     1861 agacgatcgg gcagatgatg caatagctga agcgcctaat atacctgtca ttcccgaaaa
     1921 ttccatgcgg ggtggattcg cactatgagt cgggcgacgg ctcgaagtcg agaacctctt
     1981 tcttcggcat cagctcaacg ttgccctgcg gcgtgcaccg cgccgtctgg agctgcgcgg
     2041 gagcaaccga accctcatag tccccaccgt ctcgcgcttg gcgttgatgg gcttgcgggc
     2101 gccctccgga tcattgcagc aacgcagcgg atctgcttgt tgttacggca cagcgatgca
     2161 aagccctatt cgactgctgc cgagagaacg ggatcaacct ctcgatgtta tcgagagcgc
     2221 ggcacgagac cgcgtccatg cgcaataaaa atcttgggaa aggacgccag gaacccgaat
     2281 gcagaacttc ccggccgaga cgaaagcgac atccgcatcg attactgctg gggcactttc
     2341 ggcgcggagc gcgtgcagtc tgcggtgaca gaactgctca gcttggcgcc agttctgttt
     2401 ctatcatacg actcgccgat tttgggggca ctccggcggc agacgcgcag cattccaatc
     2461 gtgttcgtcg gaattgttct ccggctacac ttttctccgc gtcgtgatgg gatggcaggc
     2521 gcgctggacg cctggcacgt ttccgggcct ttgcgcgact tccgctgcaa cgactttttg
     2581 gcagagacga attcgtccga gagaggcgtg tgtggtgtgc gagtctcgtt tgcggatctc
     2641 ggaggtacgg cagtctcttg ctcagaagca gaatttccgc gagagagggc gcgactcgaa
     2701 atgagagctc gtcgccgaac gccgggttat cggatcgcaa ggatgcaccc gtcatgatga
     2761 tgttggcacc ggttctcacg ccgcatggct tactgatgct cgcgcaatcg ttggaggcgg
     2821 aggaggaagc gctggctttg gagccagagc gcggctcgcg gttggagaag gcgtttgcac
     2881 gcggcgccgg tcacggactg ctgctcctgg gcgctgacga ggtcgggacg gctctttctc
     2941 caacgctgtc ctattggcgg aaattggggg cgcgttacat gactgcgttg tgcgcccttc
     3001 caggcatcgg cgagcgaagc aagccgccgg tgccgatccc cgcagacggc gaattggact
     3061 cgatggccgc ggccgttccg ccgatgattg gcgcggaata cgtgaccacg gaagtcctgg
     3121 tcaatctatg gcgcggcatg gattcggcct tcgataccga gctggctcaa tccaagctca
     3181 cagtgcagga gttcctcaag cgccgtcatc cggcctggaa cttagtgggg cgtgttcatt
     3241 tcaaccttgc ggagaatcgg aaagacgagg acgccccatt cgcgtttctt gcaacctaca
     3301 cgacgcggct ttcagccgag gcaaggccgc agcatctgcc gctcggcaag gccttgcagg
     3361 agtactcggg cgcgagaaac cgcgagcgcc tgctatcgct cctgatgccc gtccagcggg
     3421 ccgccgagca ttgcccctgg ttgaaggcga tggtcgatgc gggcgaaatc tttcacccgc
     3481 tgcgctggag cccacaacag gcggtggaat tcctgcggga cgttcccgcg ctcgaaagcg
     3541 ccggcgtggt cgtgcgcatg ccggcaagct ggcgcatgaa tcgtccagca cgcccgcagg
     3601 tgatggcaac ggtcggcggc aatgccccct cgcatctcgg gatggatgcg ctgctcgact
     3661 ttcggatgga ggtgacgctc gatggtgaaa acctcacggc ggtcgagatc aagcgcctcc
     3721 tggcgcattc cgacgggt
//
LOCUS       DATLJT010000700         5208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_961368,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000700 DATLJT010000000
VERSION     DATLJT010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961368"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VM910_16025"
     CDS             <1..681
                     /locus_tag="VM910_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI64072.1"
                     /translation="RKQALVAQKVGSQLDLDNSANAMVTAGAQAQFVRQQLSNAKAQL
                     LGNPDLPMEQFPPYAQAKAQLDMAQRNLDHTVLRAPMPGIATQVEQIQLGRFVAAGTP
                     VFSVIDVDQPWVDANPKESDFTYVAVGQPATLEVDAFPNHLFKGHVGSLSPGTGAQFA
                     ILPPQNATGNFVKVVQRVPVRIYFDMDDPFVKKLKAGMSVYATVDTGHKRSLATLLGF
                     APAVANQE"
     gene            689..2251
                     /locus_tag="VM910_16030"
     CDS             689..2251
                     /locus_tag="VM910_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HVI64073.1"
                     /translation="MSPTTVPSASMVPGLRRNMVTICAMTATIMQALDTTIANVALPY
                     MQGTLSASQDQINWVLTSYIVAAAIMTAPVGWIANRFGRKRIFILCSAGFTVASVLCG
                     LAQDINQMVLFRLLQGVFGAALVPLSQAVMLDSYTLQERAKAMSIWGMGVMMGPIMGP
                     SLGAWLTETYSWHWVFFVNLPFGAITVLGLVIFMDETKKNLTLRFDWFGFTALAVAIG
                     SLQLALDRGEQLGWLESNEIIAEFIISAVGFYYFFAHSLTTQNAFIQFALFKDRNFIT
                     GCIFMAVMGLVLYSTMALASPYLQNVIGYPIITAGVLLASRGCGTFVAMMMVGRLMRY
                     IEARTLIISGLLLTATSLFYMTGWTDQTGAPEIVSWSIIQGFGFGLVFVPLSTVSFLT
                     LPSHLRTDGTSMLTLMRNVASSIGISLVIAQLTQGSRRIHAILVEHVNPFNHAMQMPD
                     VSGMINMGTDTGRAMADAMVNVQAQIIAFSQDYQMVMLFILCTVPLAIMIGSTKAALR
                     SQAAGPEHAVME"
     gene            complement(2329..2919)
                     /gene="pcaG"
                     /locus_tag="VM910_16035"
     CDS             complement(2329..2919)
                     /gene="pcaG"
                     /locus_tag="VM910_16035"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HVI64074.1"
                     /translation="MSGITPSQTVGPFFKYGLTSNGQYDWNDAFTNNLVTPDASGDRI
                     RVEGKVFDGDGALIPDCMLEIWQADSQGRFADPQDKRALPNAAFKGFGRCGTDANGAY
                     AFDTIKPGAVPDPDGKPQAPHLLLAVFARGMLRHLYTRIYFGDEAGNSSDPVLTLVPA
                     DRRATLIAQRKPGNGNAVYSLDIRLQGDGETVFFDV"
     gene            complement(2916..3620)
                     /gene="pcaH"
                     /locus_tag="VM910_16040"
     CDS             complement(2916..3620)
                     /gene="pcaH"
                     /locus_tag="VM910_16040"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HVI64075.1"
                     /translation="MTFVYPVESNRAHPLPLSPDYRSSIKRAPQKPLIPMRHTLSELT
                     GPVYGHETVRAGDSDLTTQHKDEPLGERIIVHGHVLDEDGRGVPNTLLEIWQANACGR
                     YIHVRDQHPAPLDPNFTGAGRAKSDAAGYYKFVTIKPGAYPWGNHHNAWRPAHIHFSV
                     FGHSFISRLVTQMYFPGDPLFAFDPIFNAVPDEKARARMISSFDLENTQPEWALCYRF
                     NIVLRGKNATPMENKQ"
     gene            complement(3695..4903)
                     /gene="pcaF"
                     /locus_tag="VM910_16045"
     CDS             complement(3695..4903)
                     /gene="pcaF"
                     /locus_tag="VM910_16045"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HVI64076.1"
                     /translation="MRDVYVCDAVRTPIGRFGGSLAKVRADDLAAAPIKALIANHPKL
                     DWSQLDEVYLGCANQAGEDNRNVARMALLLAGLPETVPGQTINRLCASGLDAVGAAGR
                     AIRAGEVELAIAGGVESMTRAPFVMGKAPEAFSRSAEIFDTTIGWRFINPLMKAQYGV
                     DAMPETGENVAEEFQVSRADQDAFAIRSQQRAGKAIGSGYFAAEITPISVPGGKAGPV
                     IVDKDEHPRPDTTIEGLAKLKPIVRNPGTVTAGNASGVNDGAAAMILASAAAVKKHGL
                     TPRARILGLASAAVPPRIMGIGPVPATRKLMERLGIKISDFDLIELNEAFASQGIACL
                     RQLGVKEDADFVNPHGGAIALGHPLGMSGARLALTAVHGMETRGGKLALATMCVGVGQ
                     GVAVAIEKLN"
BASE COUNT          912 a         1678 c         1637 g          981 t
ORIGIN      
        1 cgcaagcagg cgctggtcgc ccagaaggtc ggctcgcagc tcgatctcga caattcggcg
       61 aatgccatgg tgaccgcggg cgcgcaggcg caattcgtgc ggcagcagct ttccaatgcg
      121 aaggcgcaac tgctcggcaa tcccgatcta ccgatggagc agttcccgcc ttatgcgcag
      181 gccaaggcgc aactcgacat ggcgcagcgc aatctcgacc acaccgtgct gcgcgcaccg
      241 atgccgggaa ttgcgacgca agtcgagcag atccagctcg gccgtttcgt cgccgccggc
      301 acgccggtat tttccgtcat cgacgtcgac cagccctggg tcgacgccaa cccgaaggaa
      361 agcgacttca cctatgtcgc agtcggtcag ccggcgacgc tggaggtcga tgcattcccc
      421 aatcacctct tcaagggtca tgtcggctcg ctctcacccg gcaccggcgc gcaatttgcg
      481 atcctgccgc cgcaaaacgc gaccggcaat ttcgtcaaag tcgtgcagcg ggtgccggtg
      541 cggatctatt tcgacatgga cgatccgttc gtgaaaaagc tgaaggccgg catgagcgtt
      601 tatgccaccg tcgataccgg gcataagcgc tcgctcgcga ccctgctcgg cttcgcgccc
      661 gcggtggcga accaggagta gacgcgcgat gtcgccgacc acggtgccat ctgccagcat
      721 ggttccgggc ctgcgccgga acatggtgac gatctgcgcg atgaccgcga ccatcatgca
      781 ggcgctcgac accacgatcg ccaacgttgc gctgccctat atgcagggca cgctgtcggc
      841 gtcgcaggac cagatcaact gggtgctgac ctcctatatc gtcgcggccg cgatcatgac
      901 cgcgccggtc gggtggatcg ccaatcgctt cgggcgcaag cgcatcttca tcctgtgctc
      961 ggccggattt accgtcgcct ccgtgctgtg tgggctggcg caggacatca accagatggt
     1021 gctgttccgc ctgttgcagg gcgtgttcgg cgccgcgctc gtgccgctgt cgcaagcggt
     1081 catgctcgac tcctacacgc tgcaggaacg cgccaaggcg atgtcgatat ggggcatggg
     1141 cgtgatgatg ggcccgatca tggggccgtc gctcggcgca tggctgaccg agacctattc
     1201 ctggcattgg gtgttcttcg tcaacctgcc gttcggcgcc atcacggtgc tcggcctcgt
     1261 cattttcatg gacgagacca agaagaatct caccctgcgc ttcgactggt tcggcttcac
     1321 ggcgctggcg gtcgcaatcg gctcgctgca gctcgcgctc gaccgcggcg aacaattggg
     1381 atggctggag tccaacgaga tcatcgccga gttcatcatc tccgccgtcg gcttctatta
     1441 tttcttcgcg cactcgttga cgacgcagaa tgcattcatc cagttcgccc tgttcaagga
     1501 ccgcaacttc attaccggct gcatcttcat ggcggtgatg gggctcgtgc tgtactcgac
     1561 catggcgctc gcctcgccct atttgcagaa cgtgatcggc tatcccatca tcaccgcggg
     1621 cgtgctgctc gcgagccgcg gctgcggcac ctttgtggcc atgatgatgg tcggacggct
     1681 gatgcgctac atcgaggcgc gcacgctgat catctcgggc ctgctgctca ccgcgacatc
     1741 gctgttctat atgaccggct ggaccgacca gaccggcgcg cccgagatcg tgagctggag
     1801 catcatccag ggcttcggct tcggcctcgt gttcgtgccg ctctcgaccg tatcgttcct
     1861 gacgctgcca agccatctgc gtactgacgg cacctcgatg cttacgctga tgcgcaacgt
     1921 cgccagctcg atcggcatct ccctcgtgat cgcacagctc acgcaaggat cgaggcggat
     1981 tcacgccatc ctcgtggagc acgtcaatcc gttcaatcat gcgatgcaga tgcccgacgt
     2041 gtcgggcatg atcaacatgg gcaccgacac gggacgtgcc atggcggacg cgatggtgaa
     2101 cgtgcaggcg cagatcatcg cgttttcgca ggactaccag atggtgatgc tgttcatcct
     2161 ctgcaccgtc ccgctcgcga tcatgatcgg ctccaccaag gccgcgttgc gcagccaggc
     2221 agccgggccg gaacatgcgg tgatggagta ggacactcgt cgtgcgccgg cttgccgtct
     2281 tcgctaaagc ttcgccagcc gacagtcaga attcgaaggt agcgccgcct acacgtcgaa
     2341 aaaaacggtc tcgccgtcgc cctgcaggcg gatgtcgagc gaatagaccg cgttgccatt
     2401 gcccggcttg cgctgcgcga tcagggttgc gcggcggtcg gccggcacca gcgtcagcac
     2461 gggatcgctg ctgtttcccg cctcgtcgcc gaaatagatc cgcgtataga gatggcgcag
     2521 catgccgcgc gcgaatacgg cgagcagcag atgcggcgcc tgcggcttgc cgtcggggtc
     2581 gggcaccgcg cccggcttga tggtgtcgaa ggcgtaagcg ccgttcgcat ccgtgccgca
     2641 acggccaaaa cccttgaagg ccgcgttcgg cagcgcccgc ttgtcctgcg gatcggcaaa
     2701 gcggccctgg ctgtcggcct gccagatctc cagcatgcaa tccggaataa gcgcaccatc
     2761 gccgtcgaac actttgcctt cgacccggat gcggtcgccg gacgcgtcgg gcgtcacgag
     2821 attgttggtg aacgcgtcgt tccagtcgta ctggccgttc gacgtcagcc cgtatttgaa
     2881 aaacggaccg acggtctgcg atggcgtaat cccactcact gcttgttctc catgggcgtg
     2941 gcgttcttgc cgcgcagcac gatgttgaag cggtagcaca gcgcccattc cggctgggtg
     3001 ttttccagat cgaacgacga aatcattcgc gcgcgtgcct tctcgtccgg cacagcgttg
     3061 aagatcggat cgaatgcaaa aagcggatcg cccggaaaat acatctgggt caccagccgc
     3121 gagatgaagg aatggccgaa caccgagaaa tggatgtgag ccggccgcca ggcgttgtgg
     3181 tggttgcccc aggggtaagc accgggcttg atggtgacga acttgtagta gccggcggcg
     3241 tccgatttcg cgcggccggc gccggtaaaa ttcggatcca gcggcgccgg gtgctggtcc
     3301 cgaacgtgga tgtagcggcc gcaggcattg gcctgccaga tctccagcag cgtgttcggc
     3361 acgccccggc cgtcctcatc gagcacatgc ccgtgcacga tgatgcgctc gccgagcggc
     3421 tcgtccttgt gctgggtggt gagatcgctg tcgccggcgc gcacggtctc gtggccatag
     3481 accggaccgg tcagttccga cagcgtgtgg cgcatcggga tcaacggctt ttgcggtgcg
     3541 cgtttgatcg agcttctata gtctggcgac agcggcaggg gatgcgcgcg gttgctctcg
     3601 accggataga cgaatgtcat gatgcttccc tcccggcatg accggccttg ccgaattatt
     3661 ataactataa ctaatccgga aaagcttttc cggatcagtt cagcttctcg atcgcgacag
     3721 cgacgccttg gccgacacca acgcacatgg tggcgagcgc gagcttgccg ccacgcgttt
     3781 ccatgccgtg caccgccgtc agcgcaagcc gcgcaccgct catgccaagc ggatggccga
     3841 gcgcgatcgc gccgccatgc ggattgacga aatcggcatc ctccttcacg ccgagttgac
     3901 gcaggcacgc tatgccctgg gacgcgaagg cctcattgag ctcgatcaaa tcgaagtcgc
     3961 tgattttgat cccgagccgt tccatcagct tgcgggtggc gggcacgggg ccgatgccca
     4021 tgatgcgcgg cggcaccgcg gcggaggcaa gacccagaat cctggcgcgc ggcgtcagcc
     4081 catgtttttt cacggccgcc gcggaggcga ggatcatcgc ggccgcgccg tcattgacgc
     4141 cggaggcgtt gccggcggtc acggtgccgg gattgcgcac gatcggcttg agcttcgcga
     4201 gcccttcgat cgtggtatcg ggacgcggat gctcgtcctt gtcgacaatg acaggaccgg
     4261 ccttgccgcc gggcacggag atcggcgtga tttctgccgc gaaatagccc gagccgatcg
     4321 ccttgccggc gcgttgctgc gagcggatcg cgaacgcatc ctggtcggcc cgcgaaacct
     4381 ggaattcttc ggcgacattc tcgccggttt cgggcatggc gtcgacgcca tactgcgcct
     4441 tcatcagggg attgatgaaa cgccagccga tcgtggtgtc gaaaatctcg gccgagcgcg
     4501 aaaacgcctc cggcgccttg cccatcacga acggcgcccg cgtcatcgac tcgacgccgc
     4561 cggcgatcgc aagctcgacc tcgcccgcgc ggatcgcacg gcctgcggcg ccgaccgcgt
     4621 ccaggccgga tgcgcaaagc cggttaatgg tctggcctgg aaccgtctcc ggtaggcccg
     4681 ccagcaacag cgccatgcgc gcgacgttgc ggttgtcctc gccggcctgg ttggcgcagc
     4741 cgagatagac ctcgtcaagc tgggaccagt cgagcttggg gtggttcgct atcaacgcct
     4801 tgatcggggc cgccgcgaga tcgtctgcgc gcactttcgc cagcgaaccg ccgaagcggc
     4861 cgatcggagt tcggacggca tcgcacacat aaacatcacg catggtttct ctccctgggc
     4921 cgctaaaggc gaggcggaat ttgatagcgg ttttaggagc gcgcctaccc aaggtcaatt
     4981 gacgcttttg cgcccgatcg tgcgttcgcg cagggccggt catgcaatcc gaggggcgtg
     5041 gaaaacccgt aagctgccgg taaagcgata ggaggggtgc acgaaattcg ctacgttgcg
     5101 ccgcccatga ccacccagcc ctcccccgtg cccgcgccca cacccactga ggctccgcaa
     5161 gccgcagaag cgcatgcgcg cgtcacgccg atgatggaac agtatctc
//
LOCUS       DATLJT010000701         6594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_731342,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000701 DATLJT010000000
VERSION     DATLJT010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731342"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VM910_16050"
     CDS             complement(<1..845)
                     /locus_tag="VM910_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI64077.1"
                     /translation="MSALQPFRLPSGGLIDRSRILRFRFDGDEVTGFAGDTLASALLA
                     KGTRIVGRSFKYHRPRGILSAGSEEPNALVELRSDARREPNTKATTIELYEGLEARSQ
                     NRWPSLKFDVLAINSLLSPIFVAGFYYKTFMWPSSFWEKLYEPIIRRTAGLGRASGAG
                     DPDRYEHAYAFCDVLVIGAGPAGLASALVAARSGARVIVCDEDFCFGGRLLAERRDMG
                     GTPGHVWTRRVVDELASLPDVRVMPRTTVFGVYDGGTYGALERVADHLPAPLPYQPRQ
                     RLWKIV"
     gene            complement(842..1123)
                     /locus_tag="VM910_16055"
     CDS             complement(842..1123)
                     /locus_tag="VM910_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HVI64078.1"
                     /translation="MRICCPYCGDRDLREFVYLGDASLKRPDPNAPDALEQFTAYVYQ
                     RDNTAGAHRDFWYHSAGCHAWLVVTRNTLTHEIIAVESAKEIRHETAGR"
     gene            complement(1133..2383)
                     /locus_tag="VM910_16060"
     CDS             complement(1133..2383)
                     /locus_tag="VM910_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HVI64079.1"
                     /translation="MRYSVLSLAWNAITGHKSWPPAWRDATPRKTYDVVIIGGGGHGL
                     ATAYYLAKKHGITNIAVLEKSWIGSGNAGRNTTIIRSNYLLSGNIPFYEWSMKLWEGL
                     EQDLNYNSMVSQRGVLNLYHSDIQRDAFARRGNAMRLHGVDAELLDRDEVRALVPYLD
                     FDNARFPIHGGLLQRRGGTARHDAVVWGYARAADARGVDIVQNCEVTGIRVSNGRVSG
                     VETTRGTISADKVALAVAGSSSRLAAMAGLRLPIESHVIQAFVSEGVKPLIGQVITFG
                     AGHFYISQSDKGGLVFGGDIDGYNSYAQRGNLPTVEDVCEGGMAVMPMIGRLRILRSW
                     GGVVDMSMDGSPIIDHTPIDGLYLNAGWCYGGFKATPASGWCFAHLIARDEPHTLAAA
                     YRLDRFAAGHLIDEKGVGAQPNLH"
     gene            complement(2384..3688)
                     /gene="glnT"
                     /locus_tag="VM910_16065"
     CDS             complement(2384..3688)
                     /gene="glnT"
                     /locus_tag="VM910_16065"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HVI64080.1"
                     /translation="MPSLGEIAKKQQIKYFLISFLDLFGTMRAKIVPAGAIDAVAKSG
                     AGFAGFATWFDMTPADPDILAMPDPDSLIQIPWKPEVAWVAADLVMDGKEVTQNPRQV
                     LKRVVATAAKEGYEFKSGVECEYFMITPDGKAISDAADQQSKPCYDQQALMRRYDVVA
                     EICDAMTAIGWKPYQNDHEDANGQFEMNWEYDSALRTADRHAFFKYMVKSIAEKHGLR
                     ATFMPKPFINLTGSGCHVHASLWSTKAKKNVFDAPKGELGLSQIAYHFVGGIMHSAEA
                     LCAITNPTVNSYKRINAPVTASGATWAPNMVTYSGNNRTHMIRIPEAGRFELRLADGA
                     ANPYLLQAALLVAGLDGIKNKRNPGKRYDINMYTEAHKARDAKLLPLNLLDALRALEK
                     SSVFEAGLGKEFLSSYLKLKHDDWNSYSRSLTDWERQTTLDC"
     gene            complement(3729..5063)
                     /locus_tag="VM910_16070"
     CDS             complement(3729..5063)
                     /locus_tag="VM910_16070"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003594873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HVI64081.1"
                     /translation="MDAEDRWIHTAPRKSATFDDYSISEIRRAAATGIYDIRGFGAKR
                     KLPDLDDLLFLGASISRYPLEGYREKCATDVTIGARFAKKPVKLKIPITIAGMSFGAL
                     SAQAKEALGRGASAVGTSTTTGDGGMTAEERAHSSILVYQVLPSRYGMNLDDLRKADA
                     IEVVVGQGAKPGGGGMLLGHKISARVAEMRTLPEGIDQRSACRHPDWTGPDDLEIKIH
                     ELREITDWEKPIYVKVGASRPYYDVALAIKAGADAIVLDGMQGGTAATQEVFIEHVGI
                     PTLAAIRPAVQALQDLGMHRKVQLIVSGGIRNGADVAKCLALGSDAVSVGTAALIALG
                     DNDPHLEDEYRKLGTTAGAYDDWHEGRDPAGITTQDATLAERLDPAIAGRKLANYLSV
                     MTLEVQTIARACGKSHVQNLEPEDLVALTVESAAMARVPLAGTNWIPGQEGM"
     gene            complement(5063..5752)
                     /locus_tag="VM910_16075"
     CDS             complement(5063..5752)
                     /locus_tag="VM910_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HVI64082.1"
                     /translation="MPTIDLAHTPLRELNAALHRLKFDSNETHWRVLNPRGQHAVAVG
                     IDAAILVEIEGHVGYYCAGMNKAATVVINGNAGPGVAENMMSGQVHVKGDVSQAAGAT
                     GRGGLLVVDGNAAARCGISMKGIDIVVKGSVGHLSAFMAQAGSLVVLGDAGEALGDSI
                     YEAHLYVGGTVKSLGADCVHKPLRDHHRAELARLLKAGGVKGEIDISRFRRYGSARRL
                     YQFHIDNIGSY"
     gene            complement(5752..>6594)
                     /locus_tag="VM910_16080"
     CDS             complement(5752..>6594)
                     /locus_tag="VM910_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HVI64083.1"
                     /translation="GMLVAGMLGTLCDRGPDSAGFAIYGSETPGAIKLTLRAPADHDL
                     DRLTKQLETAARASLPRVVRDTHMVVTLPLACEPAIRATLVELAREVSVVGAGRRMEI
                     FKEIGRPDQVASRFGLPGMTGTHAIGHTRMATESAVTTNGAHPFTTGPDQCLVHNGSL
                     SNHNAIRRELARHGLAFQTENDSEVAAGYLTWKMREGLSLDRALEASLAALDGFYTFV
                     VGTESGFGVLRDPIACKPAVMAETERWVAFGTEYRALVGLPGIDVARVWEPEPATVYF
                     RERQ"
BASE COUNT         1311 a         2054 c         1948 g         1281 t
ORIGIN      
        1 acgattttcc agagccgttg acgcggttga taagggagcg gagcgggcag atggtcggcg
       61 acgcgttcaa gggcgccgta ggtgccgcca tcgtagacgc cgaacacagt ggtgcgcggc
      121 atcacgcgca cgtccggcag acttgcgagc tcgtcaacaa cgcgtctcgt ccagacatgg
      181 cccggcgtgc cgcccatgtc ccggcgttca gcaagcagtc gaccgccgaa acagaagtcc
      241 tcgtcgcaaa caatgacccg cgcacccgaa cgcgccgcca cgagtgccga tgccaggcct
      301 gccgggcctg cgccgatgac gagcacgtcg cagaacgcgt aggcatgttc gtaacggtcc
      361 ggatcgccag cgccgctggc gcggcccaag ccggcggtgc ggcggatgat gggctcgtag
      421 agcttctccc agaagctcga tggccacatg aaagtcttgt aatagaaacc agcgacgaag
      481 atcgggctca acagcgaatt gatcgcgagg acatcgaatt tcagcgaagg ccagcggttt
      541 tggctccgcg cctcaagccc ctcatagagc tcgatcgtgg tggctttagt attcggttcg
      601 cgtcgtgcat cggaacgcag ttccacgagc gcgtttggct cctcggagcc cgcgctcagt
      661 atgccccggg ggcgatgata cttgaagcta cgtccgacaa tacgcgtccc tttcgcgagc
      721 aacgcagatg ccagcgtatc gccggcaaag cccgtgacct cgtcaccgtc gaaacgaaaa
      781 cgaagtatcc gcgagcggtc gatcaggccg ccactcggca gccggaaggg ttggagggcg
      841 ctcatcgtcc agcagtctcg tgacgtattt ccttcgccga ttccacggcg ataatttcgt
      901 gcgtgagtgt attgcgggtg accacgagcc aggcatgaca gccggctgag tggtaccaga
      961 agtcccgatg tgcgccggcc gtgttgtcgc gctgatagac gtaagccgta aattgctcga
     1021 gtgcgtcggg cgcattcggg tccggacgct tgagagaagc gtcacccaga tagacgaatt
     1081 cacgcaaatc acggtcgccg cagtaggggc aacagattcg catcgcaatc tctcaatgca
     1141 ggttgggttg cgcgcccacc cccttctcat cgatgagatg accagccgcg aaacgatcca
     1201 gacgataggc agcggcaagc gtatgaggtt catcccgcgc gatcagatgc gcaaagcacc
     1261 agcccgacgc cggagttgcc ttgaagccgc cataacacca gccagcattg agatacagcc
     1321 catcgatcgg cgtgtggtcg atgatcggtg agccgtccat ggacatatcc acgacgccac
     1381 cccatgaacg caggatccgc aagcggccga tcatcggcat gaccgccatt ccgccctcgc
     1441 agacatcctc gaccgtcggc aggttcccgc gttgggcgta ggaattgtag ccgtcgatat
     1501 ctcctccgaa caccaagcca cccttgtcgg actggctgat gtagaagtgc ccggcaccga
     1561 aagtgataac ttggccgatc agcggcttta ccccttccga gacgaatgct tgtatcacgt
     1621 gactctcaat cggcaggcgc aagcctgcca tggcggcgag ccgggaactg ctaccggcga
     1681 cggcgagcgc aaccttatcg gcgctgatcg ttcctcgcgt cgtctcgaca ccggagacgc
     1741 ggccgttgga aacgcgaatg ccggtgactt cgcagttctg gacaatatcc acgccgcgag
     1801 catccgcggc tcttgcgtat ccccacacga cggcgtcatg ccgcgccgtc ccgccgcgcc
     1861 gttggagcaa gccgccgtgg atcggaaacc gcgcgttgtc gaaatcgaga tagggcacca
     1921 gcgcccgtac ttcatcccga tcgagcagtt ccgcgtcgac gccgtgaagg cgcatggcgt
     1981 tgccgcgtcg ggcaaacgcg tctcgctgaa tgtctgaatg atagagattc agaacaccgc
     2041 gctgactcac catgctattg taattgagat cctgctcgag gccctcccag agcttcatcg
     2101 accattcgta gaagggaatg ttccccgaca acagataatt tgaccggatg atggtcgtat
     2161 tccgacccgc attgccagat ccgatccagc ttttctccag cacggcgatg ttcgtgatcc
     2221 cgtgcttttt tgcaagatag tacgcggtag ccaacccgtg cccaccaccg ccaataatca
     2281 cgacatcgta ggtctttcgc ggtgtggcgt cacgccacgc aggcggccag ctcttatgac
     2341 cggtgatcgc attccacgcc agcgacagaa ccgagtagcg catttagcaa tcgagtgtgg
     2401 tttgtcgctc ccaatcggtg agagagcggg aataactgtt ccagtcgtcg tgcttgagct
     2461 tgaggtagga tgacaggaac tccttaccga gcccagcttc gaataccgag gatttctcaa
     2521 gcgcacgcaa tgcatcaagc aggttcaacg gcagcagctt agcgtctcgc gctttatgag
     2581 cttcggtata catattgatg tcgtacctct tgccgggatt gcgcttgttc ttgataccgt
     2641 caagaccggc caccaataga gctgcctgta gtagatatgg attagctgct ccgtcggcga
     2701 ggcgaagctc gaagcgtccg gcctccggaa tacgaatcat gtgagtccga ttgttgccgg
     2761 aataagtaac catgttcggg gcccaggttg ctccggacgc ggtgactggc gcgttgatgc
     2821 gcttgtagga attcaccgtc ggatttgtga tggcgcacaa agcctccgcc gaatgcatga
     2881 tacccccgac gaaatgatag gcgatctgcg aaagaccgag ttcacccttc ggggcatcga
     2941 agacattctt cttggccttg gttgaccata gcgacgcgtg cacgtggcaa ccgctgccag
     3001 tgagattgat gaaaggcttc ggcatgaagg tcgcacgcaa cccgtgcttc tccgcgatcg
     3061 acttcaccat gtatttgaaa aaggcgtgcc gatcggcggt ccgcaaggcg ctatcgtact
     3121 cccaattcat ctcgaattgg ccgttcgcat cctcgtgatc gttttggtag ggcttccagc
     3181 cgatggcggt catggcgtcg caaatttcgg caacaacgtc gtagcgacgc atcaaggctt
     3241 gctgatcgta acaaggcttg gattgctggt cggcggcgtc ggaaatggct ttgccgtccg
     3301 gcgtgatcat gaagtactcg cattcgacgc cggacttgaa ctcatagcct tctttcgccg
     3361 ccgtcgcgac cacgcgcttg agcacctgcc gcggattctg ggtgacctcc tttccgtcca
     3421 tgacgagatc agcagcgacc caggccacct ccggcttcca gggaatttga atcaggctgt
     3481 ccgggtcggg cattgccaag atgtccgggt ctgccggtgt catgtcgaac caggtggcga
     3541 acccggcgaa gccggcaccg gatttggcaa ctgcgtcgat cgcgccggcg ggcacgatct
     3601 tggctcgcat agtgccgaac aggtcgagga aggaaatgag gaaatatttg atctgctgct
     3661 tttttgcaat ttcacccaac gacggcatag tgctaccccc tgtttggaat gctaccccga
     3721 acgcgaggtc acattccttc ctgtcccgga atccaattcg tgcccgcgag aggaacccga
     3781 gccatcgcgg ccgactcgac ggtgagcgcc acgagatcct ccggctcgag attttggaca
     3841 tgcgacttgc cgcaggcgcg ggcgatagtc tgaacctcga gcgtcatcac tgaaagataa
     3901 ttggccagct tgcggcccgc gatcgcggga tcgaggcgtt cggcgagcgt agcgtcctga
     3961 gtcgtgatac cggctgggtc gcgcccctcg tgccaatcat cataggcccc agcggtcgtt
     4021 ccgagcttgc gatattcgtc ttcaagatgc gggtcattgt cgccgagcgc gatcaatgcc
     4081 gcggtcccga ccgagacggc atccgaaccc agcgccaagc actttgccac gtcggcacca
     4141 ttacggatgc ctcccgacac gatcagctgg accttgcgat gcatgcctag gtcctgcaat
     4201 gcctgcaccg ctgggcggat cgcagcgagt gtcggaattc cgacatgctc gatgaacacc
     4261 tcctgcgttg cagccgttcc gccttgcata ccgtccaaga cgatagcgtc cgcaccagct
     4321 tttattgcga gcgcaacgtc gtaataaggc cgcgacgcgc ccaccttgac gtagatcggc
     4381 ttctcccagt ccgtgatctc gcgcagctcg tggatcttga tctccaaatc gtcagggccg
     4441 gtccaatccg gatggcggca ggccgagcgc tggtcgatcc cttccggcag cgtgcgcatc
     4501 tcggcaacgc gcgctgagat cttgtggccg agtagcatgc cgccgccgcc cggcttcgcc
     4561 ccttggccga cgaccacctc gatagcgtcc gcttttctga ggtcgtcgag attcatccca
     4621 tagcgcgacg gcaaaacctg atagaccagg atgctcgaat gcgcgcgctc ctcggcggtc
     4681 atgccgccgt cgccagtcgt cgtagacgtg ccaaccgcac tggcgccgcg cccgagcgct
     4741 tccttcgcct gcgccgacag agccccgaag ctcatcccgg caatagtgat cgggatcttc
     4801 aacttgaccg gcttcttggc gaaacgggct ccgatggtca cgtcggtcgc gcacttctca
     4861 cgatagcctt cgagcggata gcggctgatg gaggccccca ggaaaagcag atcgtcgagg
     4921 tccggtagct tgcgcttcgc gccgaagcct cgaatgtcat agatgccggt tgcggccgcg
     4981 cgacggattt ccgagatcga gtagtcgtca aaggtcgccg acttgcgtgg cgcggtatga
     5041 atccagcggt cctcggcgtc catcagtatg atccaatgtt atcgatgtgg aattgataga
     5101 gccggcgggc cgaaccgtag cggcgaaatc ggctgatatc gatttcgccc ttcacgccac
     5161 cggccttcag caggcgcgca agctcggcgc gatggtgatc ccgcaacggc ttgtgcacac
     5221 aatcggcacc aaggcttttc accgtgccgc ctacgtaaag atgagcctcg taaatcgagt
     5281 cgccgagcgc ctcgcctgcg tcgccgagca ccacgagact tcccgcttgc gccatgaatg
     5341 cggacagatg accgaccgaa cctttcacga cgatgtcaat gcccttcatg gagatgccgc
     5401 atcgcgccgc agcattgcca tcgaccacga gaagtccgcc gcgcccggtc gcaccggcag
     5461 cctgcgaaac gtcgcccttc acgtgcacct gtccagacat catgttttcc gcgacgccgg
     5521 gcccggcatt tccgttgatc acgacggtcg cggctttgtt cattccggca cagtagtagc
     5581 cgacgtggcc ttcgatctcg acgaggatgg cagcatcgat gccgaccgcg accgcatgct
     5641 ggccgcgcgg attgagcacg cgccaatgcg tttcattgct gtcgaacttc aacctgtgaa
     5701 gcgcagcatt aagctcgcgc agtggcgtat gggcgagatc aatcgtcggc attactgacg
     5761 ctcgcggaaa tacacggtcg ccggctcagg ttcccacacc cgtgccacgt cgattcctgg
     5821 caaaccgacg agcgcgcgat attccgtgcc gaacgcaacc catctttcgg tttcggccat
     5881 caccgctggt ttgcaggcaa tcggatcgcg caggacgccg aatccggatt ctgtaccgac
     5941 gacgaacgtg tagaatccgt caagcgcggc aagcgaagcc tccaacgccc gatcgagcga
     6001 caggccctcg cgcatcttcc aggtcaaata gcccgcggcg acttcggaat cgttttcggt
     6061 ctgaaaagca aggccatggc gggccaattc gcggcgaatc gcattatggt tcgacaaaga
     6121 gccattatgg acgaggcact ggtccggccc ggtcgtaaac ggatgggcac cattcgtcgt
     6181 caccgcggat tccgtggcca tgcgggtgtg accaatggca tgcgtgccgg tcatgcccgg
     6241 caatccgaaa cggcttgcga cctgatccgg ccggccgatc tccttgaaga tctccatgcg
     6301 gcgccctgcg ccgacaacgc tgacctcgcg cgccaactcg acaagcgtgg cacgaatggc
     6361 gggctcgcac gcgagcggca gcgtgaccac catgtgcgtg tcacgaacga cgcgcggcag
     6421 cgacgctctt gccgcggtct ccagttgttt ggtcaggcgg tcgaggtcgt gatcggcggg
     6481 cgctcgcaag gtcagcttga tcgcgcctgg cgtctcgctt ccgtagatgg caaagcccgc
     6541 ggaatcgggg ccacgatcac acaaggtgcc gagcatgccg gcaacgagca tgcc
//
LOCUS       DATLJT010000702         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1881444,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000702 DATLJT010000000
VERSION     DATLJT010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881444"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VM910_16085"
     CDS             complement(<1..416)
                     /locus_tag="VM910_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64084.1"
                     /translation="MSALVDVLREIRANLLLWLALLVAFFAAFPLLQLALLIVRFQEF
                     PNYLIVHDWPGNIARIVRMTPSIADMVSIMRDEWLIEIGSMNFAYGRGIAEWSFVLVP
                     AKAGVTLVIALLLATNAVLVHAVRKTCPLSARLGASA"
     gene            complement(413..1195)
                     /locus_tag="VM910_16090"
     CDS             complement(413..1195)
                     /locus_tag="VM910_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64085.1"
                     /translation="MSGVKTSAARWAVRLAALLAGLACWHYASSTSLNFYVRFDNVPG
                     PLQVGQALLGHLEQSAFYLHVAASLRRIAISYSLATGIGITLGLLMGRSRLVRDIVNP
                     YIELLRPIPAVAWIPLAILLWPTEEGSIIYITFLGALFPIVLNTVHGVEQTPEVLVRV
                     AQSLGASRLCIFRHVVLPGALPSICAGLAIGMGVSWFSLLAGEIISGQYGIGYFTWNA
                     YSIINYPDIVVGMLAIGLLGTASTALIQLLTRPALAWQRKAR"
     gene            complement(1353..1490)
                     /locus_tag="VM910_16095"
     CDS             complement(1353..1490)
                     /locus_tag="VM910_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64086.1"
                     /translation="MTAFYVRKPDGEFVSFLRKGEAEEFAATANGKTFSFEEAVANSG
                     S"
     gene            complement(1462..2772)
                     /locus_tag="VM910_16100"
     CDS             complement(1462..2772)
                     /locus_tag="VM910_16100"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64087.1"
                     /translation="MRQASFATVVAIRMPALMNLKCIPLLAMALLLPTASPAAAETID
                     IAIGHQSMCTDTYTGGIVIKELKLLEKHLPHTGKYADATYNISWADYASGGPITNQML
                     AGKLNFGTMGDYPLVVNGSKFQETKSLRTIYVSGTGYNLRGAGNAIVVPVSSNFHKLE
                     DLKGKAISTPVGSASWGMLIKALQDKKMPLASIELKNQAPAVGAANIATNKIDAHSDF
                     CPWSEIMEFRGTGRKIYDGSETGIPYLHGVVVRQDFAEKYPEVVVAFIKAIYDAGKWI
                     DEDPMRATDLMEKWTGVEKEVLYLYFSKGGHLTQDPTIKKAWVDTLKFDHGVLAQEKL
                     ASGLDFDSWINESYIKRAYAEMGLDYEAEKAKVVDTLVTNAGRPKEIWHARDGIFSYP
                     TMRQFLKAVAEFQATGAKLNASYVYDHPTGLKLFGNDGILCAQA"
     gene            complement(2964..>3329)
                     /locus_tag="VM910_16105"
     CDS             complement(2964..>3329)
                     /locus_tag="VM910_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI64088.1"
                     /translation="GKALRTLVMQIPALGVGLIEGLSFKGKCYSALVQMLGTRSVTDR
                     LAHLLLHLVDAYGIKVPDGILIGAAFTHADLAHMVGATRQWVTISLQRLQEQGVVACR
                     KSQIIVRRLDALSEMRGGG"
BASE COUNT          661 a         1009 c         1032 g          627 t
ORIGIN      
        1 gccgacgcgc cgagccgcgc ggagagcggg cacgttttgc gcacggcatg cacgagcacc
       61 gcgttggtgg cgagcagcaa cgcgatcacg agcgtaaccc cggctttggc cggcaccagc
      121 acaaagctcc attcagcgat accgcggccg taggcgaaat tcatcgagcc gatttcgatc
      181 agccattcgt cgcgcatgat cgaaaccata tcggcgatcg acggcgtcat gcggacgatc
      241 ctggcaatgt tgccgggcca gtcgtgcacg atcagatagt tgggaaactc ctggaagcga
      301 acgatgagga gcgcgagctg cagaagcggg aaggcggcga aaaaggcgac cagcaacgca
      361 agccacagca agaggttggc gcgaatctcg cgcaacacgt cgaccagcgc gctcatctgg
      421 ctttcctctg ccacgccagc gccgggcggg tgagcaactg aatcaaggcg gtggacgccg
      481 tacccaacag gccgatggcc aacattccga ccacgatgtc cggatagttg atgatggagt
      541 aggcgttcca cgtgaagtag ccgattccgt actggccgct gatgatctcg cccgccaaga
      601 gcgagaacca cgacacgccc atgccgattg cgaggccggc gcagatactc ggcagcgctc
      661 ccggcagcac gacgtggcgg aagatgcata ggcgcgaagc gccgagcgac tgcgccacgc
      721 gaacgagaac ttcgggcgtc tgctccacgc cgtgaacggt gttgagaacg atgggaaaca
      781 gggcaccgag gaacgtgata tagatgatcg atccttcctc ggtcggccac agcaggatgg
      841 caagcggaat ccaggcgacc gcagggatcg ggcggagcag ctcgatgtag gggttaacaa
      901 tgtcgcgaac gagccgcgaa cgtcccatca gcagaccaag cgtgatgccg atgccggtgg
      961 caagcgaata gctgatcgcg atgcggcgca ggctcgcggc aacgtgcagg tagaatgcgc
     1021 tctgctcgag atggccgagc agcgcttgcc cgacctgcag cggccccggg acgttgtcga
     1081 atcggacata gaaattgagg ctggtggagc tcgcgtagtg ccagcaagcg agcccggcca
     1141 gcagcgccgc gagccgcacg gcccagcgcg ccgccgacgt tttaacgccc gacatcgatc
     1201 ccgatcgagc atcccgggct ccgaccggcg gccggggcgt tctggcagcg ccgtcagacg
     1261 ccgcagcgga agacggcgtg tcggcgttcg cgcgatgggc gaccattgct ctcgccgtca
     1321 tgtgctgaat ctcgaaccga tcgatcagcc gatcatgacc ccgaattcgc caccgcctcc
     1381 tcgaacgaga aggtcttgcc attcgccgtc gcggcgaatt cctcggcctc gcctttgcgc
     1441 aggaagctca cgaactcgcc gtcaggcttg cgcacataga atgccgtcat tgccgaacag
     1501 cttgagcccg gtgggatggt cgtagacgta gctcgcgttg agcttggcgc cggtggcctg
     1561 gaattcggcg accgccttga ggaattgcct catcgttgga taagagaaaa tgccgtcgcg
     1621 cgcgtgccag atctctttcg gccggccagc gttggtcacc agcgtatcga cgaccttggc
     1681 cttctcggct tcgtagtcca gccccatctc ggcgtaggcg cgtttgatat agctctcgtt
     1741 gatccagctg tcgaagtcca agccggaggc gagcttctct tgcgcaagaa cgccatggtc
     1801 gaacttcagc gtgtcgaccc acgccttctt gattgtcgga tcctgagtga gatggccgcc
     1861 tttggagaaa tagaggtaga gcacttcctt ctcgacgccg gtccacttct ccatcaaatc
     1921 ggtcgcccgc atggggtctt catcgatcca tttgccggcg tcgtagatcg ccttgatgaa
     1981 ggcgacgaca acttccgggt atttctcggc gaagtcctgt cgcaccacga cgccgtgaag
     2041 atagggaatc cccgtctcgc tgccgtcata gatctttcgg ccggtgccac ggaactccat
     2101 gatctccgac cacgggcaga agtcggaatg cgcgtcgatc ttgttggtcg cgatattggc
     2161 ggcgccgacc gccggagctt gattcttcag ctcgatgctc gcgagcggca ttttcttgtc
     2221 ctgcagcgcc ttgatcagca tgccccagga ggcgctgccg accggcgtcg agatcgcctt
     2281 gcccttgagg tcctcgagct tgtggaaatt ggaactgacc ggaacaacta tcgcgttgcc
     2341 ggctcctctg agattatagc cggtgcccga gacgtagatc gtgcgcaggc tcttggtctc
     2401 ctggaacttc gagccattca cgaccagcgg ataatcgccc atggtgccga agttgagctt
     2461 gccggcgagc atctgattgg tgattggtcc gccggatgcg tagtccgccc aggagatatt
     2521 gtaggtggcg tcggcgtact tgccggtgtg cggcaggtgc ttctcgagca gcttcaactc
     2581 cttgatcacg atgccgccgg tataggtatc ggtgcacatg ctctggtgcc cgatggcgat
     2641 atcaatggtc tcggccgcgg cgggactcgc agtcggcagc aacaacgcca ttgccaaaag
     2701 aggaatgcat ttgaggttca ttaaagccgg catccgaatc gcgacgaccg ttgcaaagct
     2761 ggcttgtctc atggcctctt cctctttctc gctggcacga cgtgcgcggt tcgacggtct
     2821 ccccggtttc cgggattatt ggcctgcgtt ttgtgcgccg caaaccagga gtggcagtca
     2881 cggttattag ttgcccaaaa tcacccgttg cgcatatttt cggcagttgc gcagaagaac
     2941 gcttattcac taggcagcga gcgttacccg ccgccccgca tctccgagag ggcgtcgaga
     3001 cggcgcacga tgatttgcga tttccggcac gccacgacgc cctgctcctg caatcgctgc
     3061 aggctgatgg tcacccattg ccgagtcgca ccgaccatgt gggcaaggtc ggcgtgcgta
     3121 aacgcggcac caatgaggat tccgtcgggc accttgattc cataggcatc aaccagatga
     3181 agcagcagat gcgcgagccg gtcggtaacc gaccgtgtgc cgagcatctg cacgagggcg
     3241 gaataacact tgcccttgaa cgaaagacct tcgatcaagc cgacgccaag cgcgggaatc
     3301 tgcatcacca aggtccgcag ggccttgcc
//
LOCUS       DATLJT010000703         3158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_961702,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000703 DATLJT010000000
VERSION     DATLJT010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961702"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..2757)
                     /locus_tag="VM910_16110"
     CDS             complement(256..2757)
                     /locus_tag="VM910_16110"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI64089.1"
                     /translation="MLRCWQRATGGEGQVVLLTGEAGIGKSRLLSVLRDRLAAEAPTR
                     LRYFCSPHHQDSALYPVIAQLEQAAGFRREDTVETRLDKLEAQLALAPPRVEDFAILA
                     ELLSLPLEQRYPTFVLSAQRKKERAFEVLLHHLEALTRRQPVLFFYEDLHWIDPSSRE
                     LLDRTIERTASLPMLLIATHRPEFVPPWSGLPQVTTVTLRRLEGHAGAAIVERIAGAG
                     VLSREVAAEIVERADGVPLFVEELTKAVLEVGGSAEGIEKTLASVSPSSTAVPSVLHA
                     PLMARLDRLGPRAKEIAQIAAAIGREFSYQLLAPVAARSTDDLEEALGRLSDAGLVFS
                     RGIPPAATYTFKHALVRDAAYASLLRRRREELHARITTVLEEHFTDVVEYQPELLANH
                     CTLGGLTEKAIAYWGIAGRKSVARSAMVEAVSQLRKGLDLLPNVPDDHDRWSHERELQ
                     GALGAALYASQGPGAPETGQAYTRARYLCEQLGDTAALIPVLSGLWSHHSARSEYQIA
                     REIAGDLLRFAERKNDVSGMLVANRSMGTCLYILGEFLLSRKYFQKVLTLYDTNLHGS
                     IASVAAYDPRVAALSYLSWNLLVLGYPDQALSWSHQSLLWGRQLGHPHSLGYALTWAA
                     ILNLLRRGDQDALRLLEELTTLCSQHGFSHFLARANIMRGYVLSASGETEKGVALARQ
                     GFADMLSTGVIWNQTFFLGLMAQSCERAGQLDEASSLLSKALEIANRTGERWFESELH
                     RLRGEWLVARDQSYHGEAETCFHCALEIAKKQNAGFWELRAAMNLSSLWSQQRTPGAM
                     RKLLANVYARFGEGFDTVDLRKAKSMLDDLDVTLS"
BASE COUNT          652 a          996 c          936 g          574 t
ORIGIN      
        1 acttgagccg cgttcgggtc gcctgaagag accgaagagg aggccgtcgg cgcgggcgga
       61 gccaagggga tcatgcgcgc catcggtgag ctccgtcgct tcatctatgg tcgcaacgcg
      121 gaatgctcgg acggcaccag ccccagatag gcatcagctg ctgctcattc gccagtgacg
      181 aaagtccgcc acatcgaaaa cgacggctac gaaatgttcc cgccaaattt cacctacgat
      241 cggtcgcgag cccattcatg agagcgtgac gtccaagtca tcaagcatcg atttggcctt
      301 cctaagatca acggtgtcga agccctcgcc gaatctcgca tagacgttgg ccagtagttt
      361 tcgcattgcg ccgggggtgc gctgctggct ccataaggag cttagattca ttgcagcacg
      421 cagctcccag aacccggcat tttgtttctt tgcgatctca agggcgcaat gaaaacaggt
      481 ttcggcctca ccgtggtagc tctggtcgcg agcgacgagc cattcgcccc gcagccgatg
      541 cagctcggac tcgaaccacc gctcgccggt tctgtttgct atctccagag ccttcgatag
      601 cagtgaactt gcttcgtcga gttgtcccgc gcgttcgcaa ctctgggcca tcagaccaag
      661 aaaaaacgtc tgattccaaa tcacaccagt tgagagcatg tcggcaaaac cttgtcgtgc
      721 gagtgcgact cccttctcgg tttcgccgct cgccgagagc acatatccgc gcatgatgtt
      781 ggctctcgcc agaaaatgag agaaaccgtg ctggctacag agagtggtca attcctccag
      841 cagccgtaga gcgtcctggt cgccgcgacg gagcagattt aaaatagctg cccaagtcaa
      901 cgcatagccc aagctatgcg gatgacccaa ctgcctgccc caaagcaagg attgatggct
      961 ccaggatagg gcttggtcag gatagcccag gacgaggaga ttccacgaaa gataagagag
     1021 tgctgccacc cgcggatcat atgcggctac cgatgcgatt gatccgtgga gatttgtgtc
     1081 ataaagcgta agcacctttt gaaagtactt tcgtgacaat aagaactcac caagtatgta
     1141 taaacatgta cccatggacc ggttagcaac gagcataccg gacacatcat tctttcgctc
     1201 cgcaaagcgc agtagatccc ccgcgatctc acgtgctatc tggtactcgc tccgtgcaga
     1261 gtgatgactc cacaggccgc tgagaacggg tatcaaggcc gcggtgtcgc caagctgctc
     1321 gcacagatac cgcgcccgcg tataggcttg cccagtctca ggcgcgccag gcccttggga
     1381 ggcatacagc gcagcgccaa gcgcgccttg cagctcccgt tcgtgcgacc aacgatcgtg
     1441 gtcgtcggga acatttggca agaggtctaa tcctttgcgc agttgcgaca ccgcttcaac
     1501 catcgccgag cgcgcaacag atttgcggcc ggctattccc cagtaagcga tcgccttctc
     1561 ggtcaatccg ccgagcgtac agtgatttgc caggagctcg ggttgatact ccacgacgtc
     1621 ggtaaagtgt tcttcaagaa cggtcgtgat ccgtgcgtgc agctcttcgc ggcggcggcg
     1681 cagcaggctt gcataagccg cgtcgcggac aagggcatgt ttgaacgtgt aggttgccgc
     1741 gggagggata ccgcgggaga aaacaagacc ggcatcgcta agccggccca gcgcctcctc
     1801 gagatcgtcg gtactgcgtg cagcgactgg agctaacagc tggtaagaaa actcacgtcc
     1861 gatcgcagcg gcaatctgag caatttcctt ggcccgcgga ccgagccgat cgaggcgcgc
     1921 catgagcgga gcgtgcagca ccgacggaac cgccgtcgag gacggggaca cactggcaag
     1981 ggtcttctcg atcccctcgg cggatcctcc cacctcgagc acagccttcg tgagctcctc
     2041 gacaaatagc ggaactccat cggcgcgctc gacgatctcc gctgccacct cgcgcgacaa
     2101 caccccggcg ccggcgatgc gctcgactat cgctgcacca gcgtgtcctt cgagccgcct
     2161 cagtgtgacg gtcgtcacct gcggcaatcc gctccatggc gggacgaact ccgggcggtg
     2221 ggtcgcaatg agcaacatcg gcaggctggc cgtgcgctcg atcgtgcggt caaggagttc
     2281 gcgcgaacta ggatcgatcc agtgcaggtc ttcgtagaag aagaggactg gttgccgccg
     2341 cgtcagagct tccagatggt gcagcagcac ctcgaatgct ctttcctttt tgcgttgcgc
     2401 gctcagaacg aaggtcgggt agcgctgctc caacggcaat gacaggagtt cagcgaggat
     2461 ggcgaaatct tcaacgcgcg gcggcgccag cgcgagctgc gcctcgagct tgtcgagcct
     2521 cgtttcgacc gtatcctcgc gcctgaaccc ggcagcctgc tcgagctgag caatgaccgg
     2581 gtaaagggcg ctgtcctggt gatgcggcga gcagaaatag cggagccgcg taggggcctc
     2641 ggctgcaagc cggtcgcgca gaacactcag caaacgtgat ttgccgatgc cggcctcccc
     2701 ggtcaggagc acgacttgac cctcaccacc agtcgcccgc tgccagcagc gcaacaacag
     2761 ctcgatttcc tcctcgcgtc cgacgaacgg cgtcaggagc tcgccgcgta acgcctcgaa
     2821 gcgactgacg acgccactct ctcccgagac gcgccaaacg cgctgcggtt cggcgaaccc
     2881 ggcgagggat tgcggtccga gatcctcgaa ctcgaacaac gccccgatct gccgccgagt
     2941 ggcgtcggcg atgaccaaac tgttggtccg cgcgagcgct tgcaggcgag ccgctaggtt
     3001 cggcgtctcg ccgacgccgc cgcgctcctg cgccgcgccg gcgccgatca ggtcaccgac
     3061 cacgacaagc cccgtggcga tgccgagccg cgtctgcaat cgctcgggaa ggtcgaggcg
     3121 gccgatcgcc gctataaccg caagcccggc ccgcaccg
//
LOCUS       DATLJT010000704         2889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_503700,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000704 DATLJT010000000
VERSION     DATLJT010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503700"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VM910_16115"
     CDS             <1..999
                     /locus_tag="VM910_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64090.1"
                     /translation="SADSVRAFQRAVSEDKVSAVIASYISEVVLALEPWAARLKMPLI
                     TPGAASNEITKAIHNDYEKNKYTFHGYLTSAAQAQLVCDAAKDLLVDNLKFKSVAIMS
                     EDAAWTKPLDVGYEACLPKAGLKVVEHVRFSPDTTDFTPIFNNIEGKKPDVMVTGISH
                     VGVQPTVQWKNQQVPVAMFGISAQALSPTFWKDTNGAADGIPSLAVATPDVAVTSKTK
                     PFAAAFKAKFGTPPAYTGYTAYDDVHIIAEAIQRAGSTDPDKMVAELEKTNFEGTIGR
                     IQFYGKDDEFTHGIKSGPGFVTGLVFQWQNEKQVTVWPKAIAEGTLKFPDFVKLSQ"
     gene            complement(1103..1687)
                     /locus_tag="VM910_16120"
     CDS             complement(1103..1687)
                     /locus_tag="VM910_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI64091.1"
                     /translation="MFPGFRSQRLSVLAGLAGLAICVADVMPLRAAEPSAAGMWQKIE
                     DGKPSLYVLVVDHNGVFEGVMAKLFPSHGDAPNPVCSGCTDDRKNAPWLGISFIRDMK
                     RNGLVYEGGNVLDPRDGKIYSAKMTVSADGQTLTLRGYIGIALFGKDEVWTRLPDTAI
                     SQIDPAIVAKYLPAQAAAMKPPPGKKAQPAPAPR"
     gene            complement(1822..2850)
                     /locus_tag="VM910_16125"
     CDS             complement(1822..2850)
                     /locus_tag="VM910_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI64092.1"
                     /translation="MSEAIKTDVLIIGAGPCGLFAVFELGLLDMKAHLIDILDKIGGQ
                     CAELYPEKPIYDIPGIPYITGQGLTEQLMEQIKPFHPTFHLNEMVEKVEKIGDPAFRV
                     TTDGGKVFECKVVVISAGGGSFQPKRPPVPGIEAYEGASVFYSVRKMEQFRDKDLLIV
                     GGGDSALDWTLNLHPIAKRVTLLHRRDDFRAAPHSVEQMRALVAAGKMELKIGQVTAL
                     EGSSGVLSGATIKGNDNALSKITCDTMLPFFGLTMKLGPVANWGIELENNLVPVETSA
                     FETSVRGIFAIGDINTYPGKLKLILCGFHEGALMAQKAHRYVYPDKRLVFQYTTSSSS
                     LQKKLGVG"
BASE COUNT          584 a          892 c          874 g          539 t
ORIGIN      
        1 tcggcggatt ccgtgcgtgc gttccagcgc gcggtcagcg aagacaaggt gagcgcggtg
       61 atcgcaagct atatcagcga ggtggtgctc gcactggaac cgtgggcggc gcggctcaag
      121 atgccgctga tcacgccggg cgcggcttcg aacgaaatca ccaaggcgat ccacaatgat
      181 tacgagaaga acaaatatac tttccacggc tacctgacct cggcggcgca ggcgcaactg
      241 gtttgcgatg ccgccaagga cctgctggtc gacaatctca aattcaagtc ggtcgcgatc
      301 atgagcgagg acgcagcctg gaccaagccg ctcgacgtcg gctacgaggc ctgtctgccg
      361 aaggcaggac tgaaagtcgt cgaacacgtc cgcttctcgc ccgacaccac cgacttcacg
      421 ccgatcttca acaatatcga aggcaagaag ccggatgtga tggtcaccgg catctcccat
      481 gtcggcgtgc agccgaccgt gcaatggaag aaccagcagg tgccggttgc gatgttcggc
      541 atcagcgcgc aggcgctgag tccgaccttc tggaaggata ccaatggcgc cgccgatggc
      601 atcccgtcac tcgccgtcgc aacgcctgac gtcgccgtga cctccaagac aaaaccgttc
      661 gcagcagcct tcaaggcgaa attcggcacg ccgccggcct ataccggcta tacggcctat
      721 gacgacgtcc acatcattgc ggaagcgatc cagcgtgccg gttcgaccga tccggacaaa
      781 atggtcgccg agctcgagaa gaccaacttc gaaggcacga tcgggcgcat tcagttctac
      841 ggcaaggatg acgagttcac ccacggcatc aagtcgggcc ccggattcgt cacaggcctc
      901 gtgttccagt ggcagaacga gaagcaggtc acggtctggc ccaaggcgat cgcggagggg
      961 acgctgaaat tccccgattt cgtgaagctg tcgcaataag gtctgcctga tcctgacatc
     1021 cagcggcggg aggcgcgcat ctgcagcgcc ttctgtccgc tcacgcgcgc cgacgcaggc
     1081 ttgttgacaa cgaaacctga aatcagcgcg gtgcaggtgc cggctgcgcc ttcttgcccg
     1141 gcggcggctt catcgctgcc gcctgcgcgg gcagatattt cgcgacgatc gccggatcga
     1201 tctggctgat tgcggtgtcc ggcaggcggg tccaaacttc gtccttgccg aacagcgcga
     1261 tgccgatata gccgcgcaag gtcagcgttt gaccgtccgc gctgaccgtc atcttggcgc
     1321 tgtaaatctt gccgtcgcgc ggatcgagca cgttaccgcc ctcatacacc agtccgttgc
     1381 gcttcatgtc gcggatgaag gaaattccaa gccagggcgc gttcttgcga tcgtcggtgc
     1441 accccgagca aaccgggttt ggggcatcgc catggctggg aaacagcttg gccatcacgc
     1501 cctcgaagac gccgttgtga tcgacaacga gaacgtaaag cgacggcttg ccgtcctcga
     1561 tcttttgcca catgcctgcc gcagaaggct ccgcggcgcg cagaggcatg acgtcggcta
     1621 cgcaaatcgc caggccggca aggcccgcta gcaccgaaag tcgctggctt cgaaatccgg
     1681 ggaacatatt ccgcatcgac accacctgac caatccacaa atgaatggcc gaagactacg
     1741 gccatttcga ggatggttcc agtgccggca accgaacctg tcgagcatgt aatgtgcgtg
     1801 ccgcttcacc agcggtcgca atcagccgac cccgagtttc ttctgcaggc tcgaggacga
     1861 cgtcgtgtat tggaacacca ggcgcttgtc gggatagacg tagcgatgcg ccttttgcgc
     1921 catcagcgca ccctcgtgga agccgcagag gatcagcttg agcttgccgg gataggtgtt
     1981 gatgtcgccg atggcgaaaa ttccgcgcac gctggtctcg aacgcgctgg tttcgaccgg
     2041 caccagattg ttctcgagct cgatgcccca attggcgacg ggaccgagct tcatggtgag
     2101 cccgaagaaa ggcaacatgg tatcgcaggt gattttcgac agcgcgttgt cgttgccctt
     2161 gatggtggcg ccggacaata cgccgctcga gccttcgagc gcggtgacct gaccgatctt
     2221 caattccatc ttgccggccg cgaccaacgc gcgcatctgt tcgacgctat gaggcgcggc
     2281 gcggaaatcg tcgcgccggt gcaacagtgt gacgcgcttg gcgatcggat gcaggttgag
     2341 cgtccagtcg agcgccgagt cgccgccgcc gacgatcaaa agatccttgt cgcggaactg
     2401 ctccatcttg cgcacggagt aaaataccga agcgccctcg taggcctcga tgcccggcac
     2461 tggcggacgc ttgggctgga acgagccgcc ccccgcggag atcaccacca ccttgcactc
     2521 gaagaccttg ccgccatccg tcgtgacgcg gaaggcgggg tcgccgatct tctcgacctt
     2581 ctcgaccatc tcgttgagat ggaaagtcgg atggaacggc ttgatctgct ccatcagctg
     2641 ttcggtgagg ccctgccccg tgatgtaggg aatgccgggg atgtcgtaaa tcggcttctc
     2701 cggatagagc tcggcgcact ggcccccgat cttgtcgaga atgtcgatca gatgcgcctt
     2761 catatcgagc aggccgagtt caaagacggc aaacagtccg cagggacccg cgccaatgat
     2821 cagcacgtcg gttttgatcg cttcgctcat atcgcttctt ttcggttggg gcgcgccggc
     2881 caataaagc
//
LOCUS       DATLJT010000705         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3030376,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000705 DATLJT010000000
VERSION     DATLJT010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030376"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VM910_16130"
     CDS             <1..404
                     /locus_tag="VM910_16130"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64093.1"
                     /translation="LFILAEDPVGLGLVSSLARPGGNMTGINFLSAELTAKRLELVRE
                     LVPRAARVAAHDLREYAEVGGLMSYGTNISDAYRQMGAYAGRIIKGAKTADLPVIQSS
                     KFELVINARTARMLGLTVPPTLLAIADEVIE"
     gene            573..1364
                     /locus_tag="VM910_16135"
     CDS             573..1364
                     /locus_tag="VM910_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI64094.1"
                     /translation="MDRRSFLAAAALSAAGTAVPKIGRAAASKTYLLVHGAWHGGWCW
                     SKVALILRGRGHTVLTPTQTGLGERSHLLSKSIDLNVFVTDIANVIRYEDLNDVVLVG
                     HSFGGNAISGVADIMRDRIRQLIYLDAVILENGQSVFSQLPKDVVEARTKAAQETSGG
                     LSIPAPPPAAFGITDAAGTQFVQSRFTPHPFNTFVSPLKLANKVGNGLPTTYIICADP
                     IYKPLEASRNWVKAAGWKTIEIQCGHDAMVIAPDRLADLLDADAA"
     gene            1370..1705
                     /locus_tag="VM910_16140"
     CDS             1370..1705
                     /locus_tag="VM910_16140"
                     /inference="COORDINATES: protein motif:HMM:NF045246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64095.1"
                     /translation="MPLLFALRASESLGWLINDEDFLGRCAREAQADKLFKECLETAD
                     RRSESENENPQRIRRDQLRINTRKWMAARLAPKKYGRPYKPRCPWLNYAQLPAGHSHQ
                     GRRRNDHQN"
BASE COUNT          437 a          578 c          607 g          425 t
ORIGIN      
        1 tcctgttcat tctggctgaa gacccagtcg ggctcggcct tgtttcgagc cttgcccgcc
       61 caggcggcaa tatgacgggc atcaatttcc tctcggcgga actgactgca aagcggctag
      121 aacttgtgcg tgaactggtg ccccgcgctg cccgtgtggc cgcgcatgat ttgcgtgaat
      181 acgccgaagt cggcggactg atgagctacg gaaccaacat ttcggatgca tatcggcaga
      241 tgggtgccta tgccggccgc atcatcaagg gcgccaagac ggcggacctg cccgtcattc
      301 aatcttcgaa attcgagctc gtcatcaatg ctcggacagc ccggatgctc ggccttaccg
      361 tgccgccgac gctgctcgcg atcgcggacg aggtgatcga atgaagcgcc gcgagttcat
      421 cacgttggtt ggcggcacgg cggtcgcatg gccgctcgcg gcgagacagc tgacaattga
      481 gatcggcact ggtgcgtttt tctagacgcc catacaattg cggtgtgcgt tgacgcactt
      541 cccggcaacg cggtcacttc acggaggatg tcatggatcg tcgatcgttt cttgcggcgg
      601 cggcgctttc cgctgccgga actgcggtgc ctaaaatcgg ccgcgcggca gcatcgaaga
      661 cttacctact tgtgcatggg gcctggcacg gcggttggtg ctggagcaaa gtcgcgttga
      721 tcttgcgcgg acgaggacac actgtgttga cgccgacgca aaccgggctc ggcgagcgct
      781 cgcatttgct atcaaagtcg attgatctca acgtttttgt caccgacatc gccaatgtga
      841 ttcgatatga agatctcaac gatgttgtgt tagtcggcca cagttttgga ggcaacgcga
      901 tctcaggagt tgctgacatc atgcgcgacc gcatccgtca gcttatctac ctcgacgcgg
      961 tgatactgga gaatggccaa agtgtgttta gccagctacc taaggatgtc gtagaggcac
     1021 gcaccaaggc ggcacaggaa accagcggag gcttgagcat tccggcgccg ccgcccgccg
     1081 cctttggtat cacggacgcg gcgggaacac agttcgtaca aagccgcttc acgccgcatc
     1141 cgtttaatac cttcgtatcg ccactcaagc tggctaataa ggtcggcaat ggcctgccga
     1201 cgacctacat catctgtgcc gatccgatct ataagccttt ggaagcgagc cgcaattggg
     1261 ttaaggcggc aggctggaag acgatcgaaa tccagtgcgg gcatgatgca atggtaattg
     1321 ctcccgatcg actcgctgat ctgttggacg ccgatgcggc gtgattttcg tgcctctgct
     1381 ctttgctctt cgcgcctcgg aatctctcgg gtggctgata aacgacgagg actttcttgg
     1441 gagatgcgcg cgcgaggcgc aggctgacaa gctcttcaag gaatgcttag agaccgcgga
     1501 tcgcaggagt gagagcgaaa acgaaaaccc gcaacgtatt cggcgagacc aattgcgcat
     1561 caatactcgt aaatggatgg cagctaggct cgcgccgaag aaatacggtc gaccatataa
     1621 gccacgatgt ccgtggctca actacgctca actaccagcc ggccattctc atcaaggtcg
     1681 gcgacggaac gaccaccaga attgaagatg ctcctgcacc caaagggcgt ggcgttctgc
     1741 ttgacggcaa cgaggacatt gagcgggagt gctaacaggg gaggcacatg gccgtcgagc
     1801 ctctcgccat gtgttatcct ggtctggttg gaggcacttg aagggccggc taacgccccg
     1861 attcagaaca attcagaaca attcagatcc agcccaagga cctcatccaa ttcgcccctc
     1921 taaagtccgg cgtagcgaca tctatctggc gccaggaggg gtacatggca ccgaaaccaa
     1981 aaccagctac cgtcgggatg gttcttgcga tggcgttgac catcctagcg gccatgacgt
     2041 gggcggt
//
LOCUS       DATLJT010000706         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1881858,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000706 DATLJT010000000
VERSION     DATLJT010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881858"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1502)
                     /locus_tag="VM910_16145"
     CDS             complement(207..1502)
                     /locus_tag="VM910_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI64096.1"
                     /translation="MNDLDLRDRDDVVDRDRQDNPAVDREIKSPAKHRPPVRRRWGGR
                     LFALGGFLLLVVGLSLGVWGKISQQQQVMASAERARDFVPSVRVATTEASPGTVSVTL
                     PGTTAAFAAANIFARATGYISKRNVDIGDHVKAGELLAQLAVPELDDQISQNEATLDH
                     LKSMLAQAEANRKLQQVTRGRDAPLVEKGWVTRQQGDVDVQNLKGQEAAVAAAQHNVT
                     AQENQVKQLHQQRDYASVVAPFDGVITQRNVDVGSLVQGNATSGTFMFEIMQEDVIRV
                     SAFVPQDAAFGVAPGVDAVVRVPELPDREFPGTVTRIADALQPGTRTLLTEVDITNPD
                     GALTPGIYCTVEFHIPRKTPSLSVPADAIIFNRNGMQVAVVSNGRAELRKVNVKRDLG
                     TRVEVDTGIKAGDQVILNPPVTLVDGSKVQIRSDTASDK"
     gene            complement(1495..>2295)
                     /locus_tag="VM910_16150"
     CDS             complement(1495..>2295)
                     /locus_tag="VM910_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI64097.1"
                     /translation="TPVSTQLAANGPPIPGLLSNVATLQRDSVPTNLNQTNIQPVYDI
                     YASVQGRDLGRIAGDLGKIVAELQKELKPGNTIRVLGQIQSMHDSFRDLGSGLLFAAV
                     FVYLLMVVNYQNFGDPFVVILALPATFCGIVTMLFITGTTLNVPSLMGAIMAVGVASA
                     NSILLVTFAREQQLKGRSPFRAALDAGRTRIRPVLMTAAAMVVGMIPMAIGGPGEEQN
                     AALARAVIGGLLFATPTTLLIVPYLFAMLRKHNDGVAAYGVFEDLPDE"
BASE COUNT          406 a          723 c          732 g          434 t
ORIGIN      
        1 ctggccggga agtcgccagc ccacccagtt acacagcgct tcgcccatga caagcttgag
       61 atcgcggccc tcgagccact acaattcctc ggtgaacacg gtcacgcatt gccgccagga
      121 ccaggtctct tcaggggcaa aatccagccg ctccatcgcg gtcgcgaggt gcaagcttgg
      181 ggtcgacggc ggctgcgggt cgcaggctat ttatctgatg cagtatcgga ccgaatctgc
      241 accttgctgc cgtcgacaag cgtgaccggc ggattgagga tcacctggtc tccggccttg
      301 atcccggtgt ccacttctac ccgtgtgccc aaatcgcgct tcacgttgac cttacggagc
      361 tccgcccttc cgttgctgac aaccgccacc tgcattccgt tgcggttgaa gatgatcgca
      421 tcggccggca cggaaaggct cggtgtcttg cgcgggatgt ggaattcgac cgtgcagtag
      481 atcccgggcg tcagggcgcc gtcgggattt gttatgtcga cttccgtcaa caacgtccgt
      541 gtgccgggct gcagcgcgtc ggcgatccgg gtgaccgtgc ccgggaactc gcggtcgggg
      601 agctcgggca cgcgcacgac ggcgtcgacg cccggcgcca caccgaaggc cgcgtcctgc
      661 ggcacgaagg ccgagacgcg aatgacgtcc tcttgcatga tctcgaacat gaaggtgccg
      721 cttgttgcat tgccctgcac caggctgccg acatcgacgt tgcgttgggt gatgacgccg
      781 tcgaacggtg cgactaccga ggcatagtcc cgctgctgat ggagctgctt gacctggttc
      841 tcctgcgccg tgacgttatg ttgcgcggcg gcaactgcgg cctcctgccc tttgaggttc
      901 tggacgtcga cgtcaccttg ttggcgggtc acccagcctt tttcgaccaa tggggcgtcc
      961 cggccccgag tcacctgctg gagctttcgg ttggcctcgg cctgggccag cattgacttg
     1021 aggtgatcga gtgtagcctc gttctgcgaa atctggtcat cgagttccgg tactgcgagc
     1081 tgagccagca gttctccggc cttgacatga tctccgatgt cgacgttgcg cttggatatg
     1141 tagccggtcg cgcgcgcaaa gatattggcc gcggcgaatg cggccgtcgt gcccggcaag
     1201 gtgaccgaaa cggtgccggg gctcgcctcg gtcgtcgcga cgcgcacgct aggtacaaaa
     1261 tcacgcgccc gctcggcaga cgccatcacc tgttgctgct gcgaaatttt tccccacact
     1321 cctagagaca ggccgacgac gagcagcaaa aacccgccga gcgcgaacag ccgcccgccc
     1381 catcggcggc gcactggcgg gcgatgcttc gctgggcttt taatctcgcg gtcgaccgcg
     1441 gggttgtctt ggcgatcccg gtcgaccacg tcatcgcgat cgcggagatc aagatcattc
     1501 atcggggaga tcctcgaaca cgccataggc cgcgacgccg tcgttgtgct tgcgcagcat
     1561 ggcaaacaga tacgggacaa tcagcaaggt ggtcggtgtc gcaaacaaga ggccgccgat
     1621 cactgcacgc gccaatgccg cgttttgctc ctcgccaggg ccaccgatcg ccatcgggat
     1681 catgccgacg accatcgccg ccgcggtcat cagcaccggg cggattcggg tacggcccgc
     1741 gtcaagggcg gcccggaacg ggctcctgcc tttgagctgc tgctcgcgcg caaacgtcac
     1801 cagcaggatg gaattcgccg aggcgacgcc aaccgccatg atggcaccca tcagcgaggg
     1861 gacattgagc gtcgtgccgg tgatgaacag catcgtgacg atgccgcaga aggttgccgg
     1921 caatgccagg atcacgacga acgggtcgcc gaaattctgg taattgacga ccatcagcag
     1981 atagacgaac acggcggcga acaacaggcc ggagcccaga tcgcgaaagg agtcgtgcat
     2041 gctctggatt tgcccgagca cccggatcgt gttgccgggc ttgagctcct tctgcagttc
     2101 ggcgacgatc ttgccgaggt cgccggcaat gcggccgaga tcgcggcctt gcacgctggc
     2161 atagatgtcg taaaccggct ggatattggt ctgattcaga ttggtcggca cgctgtcgcg
     2221 ctgcaacgtc gccacattgc tcaggagccc cggtatcggc ggcccgttgg cggcgagctg
     2281 ggtcgacacc ggggt
//
LOCUS       DATLJT010000707         4879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1192743,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000707 DATLJT010000000
VERSION     DATLJT010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192743"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VM910_16155"
     CDS             complement(<1..911)
                     /locus_tag="VM910_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVI64098.1"
                     /translation="MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVS
                     QGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIIMSAQNTFMTAIRASERGAYEYL
                     PKPFDLKELIAIVGRALAEPKERTQSAAEETEFDSIPLVGRSPAMQEIYRVLARLMQT
                     DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFT
                     GANTRASGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRI
                     VAASNKDLRILIQQGLFREDLFFRLNVV"
     gene            complement(921..2093)
                     /locus_tag="VM910_16160"
     CDS             complement(921..2093)
                     /locus_tag="VM910_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HVI64099.1"
                     /translation="MTTAAERKRGPSDGEAILNALPNPVLLVAPDGKIVDANNAAESF
                     FEISTQFLQRQSLKELVPFGSPLLALIDQVRASGSPVNEYKVDLGTPRIGGDRQVDLH
                     VAPLSERPGHIVVMLQERTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGA
                     AQLLEQQASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHVKR
                     LAQSGFARSVRFIEDYDPSLPPVLANQDQLIQVFLNLVKNAAEAVVDLGGDAEIQLTT
                     AFRPGVRLSVPGKKSRVSLPLEFCVKDNGPGVPEDLLPNLFDPFVTTKQTGSGLGLAL
                     VAKIVGDHGGIIECESQPRKTTFRVLMPMFNGAKQTDQIHRDDVTGTPLHASQGTR"
     gene            complement(2090..3043)
                     /gene="dusB"
                     /locus_tag="VM910_16165"
     CDS             complement(2090..3043)
                     /gene="dusB"
                     /locus_tag="VM910_16165"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HVI64100.1"
                     /translation="MSGVTDAPFRRLAESLGAGLVVSEMTASDDLAQGRPISRLRCEA
                     TGIGPHVVQLAGCEARWMREGAKIAEEAGANIIDINMGCPARHVTGGQSGSALMRDLD
                     HATTLIEATIAAVRVPVTLKMRLGWDDRTRNAPDLARRAEAAGVKMITVHGRTRCQFY
                     KGDADWAAVREVREATSLPLVVNGDITSYESAVSALEASGADAVMIGRGAQGQPWLPG
                     QIGRRLDSGIAEAAPPLAQQLGLIRTLYDEICSHYGLRVGLRHARKHLGWALEVAGSC
                     GRAPAQRLKAWRERILTSDDPSHVHRALGEAFDDFAWSAAA"
     gene            3431..4633
                     /locus_tag="VM910_16170"
     CDS             3431..4633
                     /locus_tag="VM910_16170"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HVI64101.1"
                     /translation="MTKATAAVIVAAGRGLRAGTGGPKQYRSIGGQTVIFRAMEPFCR
                     HPQLFAVQPVVNPDDIQMFQDAVCGLSHASPTEGGATRQASVHAGLEALAPQKPDIVL
                     IHDAARPFVSQGLISRAIDAVKPTGAAVPTIAVADTIKLVDGKGNVEATPERARLRIA
                     QTPQVFYFDAILDAHRRAAREGRSDFTDDAALAEWAGLTVATFEGDVANMKLTTPEDF
                     VREESRLASALGDIRTGTGYDVHAFGAGDHVMICGVRVPHTKGFLAHSDGDVGLHALV
                     DAILGALADGDIGSHFPPSDPQWKGAASHQFLKYAVDRVSARGGRIANLEVTLICERP
                     KIGPLRDQMRARIAEISGVDISRVAVKATTSEKLGFTGREEGIAATASATIRLPWDGQ
                     EPDKKPGV"
     gene            4697..>4879
                     /locus_tag="VM910_16175"
     CDS             4697..>4879
                     /locus_tag="VM910_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVI64102.1"
                     /translation="MGSERQIGSTSALARSLLDLCRSRRLTLATAESCTGGLVAGALT
                     DIPGSSDVVDRGFVTYS"
BASE COUNT          849 a         1567 c         1567 g          896 t
ORIGIN      
        1 acgacgttga ggcggaagaa caggtcttcg cggaacagtc cctgctggat caggatacgc
       61 aggtccttgt tcgaggcggc aacgatgcgc acgtcggtct tgatcggggt gcggccgccg
      121 acggtggtgt attcgccctg ctggagcacg cgcaacaggc gggtctgcgc ctccatcggc
      181 atgtcgccga tctcgtcgag gaacagcgtg ccgccttcgg cctgctcgaa gcggccggag
      241 gcgcgggtgt tggcgccggt gaaggcgccg cgctcatggc cgaacaattc ggattcgatg
      301 aggtcgcgcg ggatcgccgc catgttgacg gcgacgaacg gtccgttgcg gcgcttgccg
      361 tagtcgtgca gcgcgcgcgc caccagttcc ttgcccgttc cggattcgcc ggagatcatc
      421 acggtgaggt cggtctgcat cagccgcgcc agtacgcgat agatttcctg catcgccggc
      481 gaacggccga ccagcgggat cgaatcgaat tccgtctctt ccgccgcgct ctgcgtccgc
      541 tccttcggct cggcgagcgc gcggccgacg atcgcgatca attccttcag gtcgaacggt
      601 tttggcagat attcgtaagc gccccgctcg gaggcgcgga tcgcggtcat gaaggtgttt
      661 tgcgcgctca tgatgatgac gggcaggttc ggccgcatct tcttgatgcg cggcagaagg
      721 tcgaaagcgt tctcgtcggg catgaccacg tcggtgatga cgagatcgcc ctcgccctgg
      781 ctcacccatc gccacagggt tgcggcgttg ccggtgaggc ggacctcgta tccggcgcgc
      841 gagagagcct ggttcagaac ggtccggatg gcggtgtcgt catcggcgac aagaatgcta
      901 cctgcaggca ttgttgttcc tcatctggtg ccctgtgagg catgcaacgg cgtcccggta
      961 acgtcatcgc gatggatctg gtcggtttgc ttggcgccgt tgaacatcgg catcagcacg
     1021 cggaaggtcg tcttgcgtgg ttgggactcg cattcgatga tgccgccgtg gtcgccgacg
     1081 atcttggcaa cgagcgcgag tccgagcccg ctgccggttt gcttcgttgt cacgaaggga
     1141 tcgaaaagat ttggcaacag atcctccggc acgccggggc cgttgtcctt cacgcagaac
     1201 tcgagcggca gcgacacccg cgacttcttg ccgggcactg agaggcggac acccgggcga
     1261 aacgccgtcg tgagctggat ttctgcatcg ccgccgagat cgaccacggc ttcggcggcg
     1321 ttcttgacca ggttgagaaa cacctggatc aactgatcct ggttggcgag caccggcggc
     1381 agcgagggat cgtagtcctc gatgaagcgg acgctgcggg caaagcccga ctgcgccagc
     1441 cgcttcacat gatcgagcac ggagtggatg ttgaccgggc cgcgcgccac aggccggtca
     1501 tcgccgaaca cttccatgcg gtcgaccagg gtcacgatgc ggtcggcctc gtcgcagatc
     1561 aggcgggtca gcatgcggtc ttcagatgag gcctgctgct ccagaagctg ggccgcgccg
     1621 cggatgcccg acagcggatt cttgatctca tgcgcgagca tcgcagcgag cgcgatcacg
     1681 ctgcgcgcgg cgctgcggtg ggtgagctgg cggtccatct tgtcggcgat ggtgcgctcc
     1741 tgcagcatca ccacgatatg gccggggcgc tcgctcagcg gggccacgtg gaggtcgacc
     1801 tgacggtcgc cgccgatccg cggcgtgccg agatcgactt tgtattcgtt gacgggcgag
     1861 ccgctcgcgc gcacctgatc gatcagcgcg agcagcggac tgccgaacgg caccagctcc
     1921 ttcagcgatt gccgttgcag gaactgggtg gatatttcga aaaaggactc ggcggcgttg
     1981 ttggcgtcga cgattttgcc gtccggcgcg accagcagca cggggttggg cagcgcgttg
     2041 aggatcgcct cgccatccga aggacctcgc ttgcgttctg cggccgtcgt catgcggcag
     2101 cactccatgc aaaatcgtcg aacgcctctc cgagcgcccg gtgcacgtgg gagggatcgt
     2161 ccgacgtcaa tatcctttcg cgccaggctt tcagtctttg tgccggggcg cgcccgcaag
     2221 aacccgcgac ctccagcgcc cagccgagat gcttgcgcgc atggcgaagc cctacgcgca
     2281 agccatagtg actgcagatc tcgtcataga gggtgcgaat aagtccaagc tgctgcgcaa
     2341 gcggcggagc tgcttccgca atgccgctgt cgagccggcg accgatctgg ccgggcagcc
     2401 agggctgccc ctgcgcaccg cgcccgatca tgacggcatc ggcccccgag gcctcgagcg
     2461 cgctgaccgc gctctcatac gaggtaatat cgccgttgac gacaagcgga aggctcgtcg
     2521 cctccctcac ctcgcgcacc gcggcccagt cggcgtcgcc cttgtagaac tggcagcggg
     2581 tgcgcccgtg cacggtaatc atcttcaccc cggcggcttc cgcgcgccgc gccaggtccg
     2641 gcgcgttgcg cgtgcgatcg tcccaaccga gccgcatctt cagggtcacg ggcactctca
     2701 cggccgcaat cgtcgcctcg atcaaagtcg tggcgtggtc gaggtcgcgc atcagcgctg
     2761 aacccgattg gccgccggtc acatgccgcg ccgggcagcc catattgatg tcgatgatgt
     2821 tggcgcccgc ctcttcggca atttttgcgc cttcgcgcat ccaacgcgcc tcgcaacccg
     2881 caagctggac gacgtgtggc cctattccag tcgcttcgca acgcaaccgg gaaattggcc
     2941 gtccctgggc gagatcgtcg ctggcagtca tctcggagac cacaagtccc gcgcccagac
     3001 tttcggctaa acgtcgaaac ggcgcatcgg tcacgcccga catcggcgcg agaagaaccc
     3061 ggttggcgag tcgaatatcg cctattttca acggcttaga gtccaaaatg tctgagccgg
     3121 tcacgaacgt ctcgtcttta tgacaggcgc ctcatcgcga ctgcctattc gtataatgag
     3181 cacaaatctt gtgcagtcaa gttgcatgcc tacacattag acaattcttg tagcgtcttg
     3241 caagtgcagt gcaacaaatt tttgccttcc cccagatgcg aaacgacacg gaaagcggcg
     3301 aggaattatc tcaggttacc gggaatcgct ctcttttgcg cacgccgcgt gataaggcct
     3361 gcggcgccgg caaacgagct cgctaacgtc aaatccgctc cacgagttat gcaacccaaa
     3421 ttgaaatccc atgactaaag ctaccgccgc cgtcatcgta gcagccggtc gcggcttgcg
     3481 tgccggtact ggcggaccga aacaatatcg ttcgatcggc gggcagaccg tcatcttccg
     3541 cgccatggaa ccgttctgcc ggcatccgca gctcttcgcg gtgcagccgg tcgtcaaccc
     3601 cgacgacatc cagatgtttc aggacgcggt atgcgggctc agccacgcct cgccgaccga
     3661 gggcggcgcg acgcggcagg cgtcggtgca tgccgggctc gaggcgctcg cgccgcaaaa
     3721 gccggacatc gtgctgattc atgatgccgc ccgccccttt gtctcccagg ggctgatttc
     3781 ccgcgcgatc gatgcggtga agcccacggg cgcagcggtc ccgacgattg ccgttgccga
     3841 cacaatcaag ctcgtcgacg gcaaagggaa tgtcgaagcg acgccggagc gcgcgcggct
     3901 gcgcatcgcg cagacaccgc aggtgtttta tttcgacgcg atcctcgatg cccaccgccg
     3961 cgcggcgcgc gagggacgaa gcgatttcac cgatgatgcg gcgctcgccg aatgggcggg
     4021 attgacggtc gcgacatttg aaggcgatgt tgccaacatg aagctcacga cacctgaaga
     4081 tttcgtccgc gaggaatccc gcctcgcaag cgcgctcggc gatatcagga ccggcacggg
     4141 ctatgacgtg cacgccttcg gcgccggcga ccatgtgatg atctgcggcg tgcgggtacc
     4201 gcataccaag ggatttctcg cccattccga cggcgatgtc ggcctgcatg cgctggtcga
     4261 tgccatcctc ggcgcgctcg ccgatggcga catcggatcg cattttccgc ccagcgaccc
     4321 gcaatggaag ggtgcggcct cgcatcagtt cctgaaatac gcggtcgacc gcgtgagcgc
     4381 gcgcggcggc cgcatcgcca atcttgaggt gaccttgatt tgcgagcgcc cgaagatcgg
     4441 ccctttgcgc gatcagatgc gcgcgcgcat cgccgagatc tcgggcgtgg atatctcccg
     4501 cgtcgccgtg aaagcgacca ccagcgaaaa gctcggcttc accggccgcg aggaaggcat
     4561 cgctgcgacc gcgagcgcga cgatccgcct cccctgggac ggccaagagc cggacaaaaa
     4621 gccaggggtt tgagatgagc ggcagcgagg ccggtggagc tttcgatttg acgttcgcat
     4681 gagggtatcg cggcagatgg gtagcgaacg tcaaatcggc tcgactagtg cactggcccg
     4741 ctcgctgctc gatctctgcc gcagtcgaag gctgacgctc gcgaccgccg aatcctgcac
     4801 cggcggtctc gttgccggtg cgctcaccga catccccggc tcctccgatg tcgtcgaccg
     4861 cggcttcgtc acctattcc
//
LOCUS       DATLJT010000708         2885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4179853,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000708 DATLJT010000000
VERSION     DATLJT010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179853"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VM910_16180"
     CDS             <1..712
                     /locus_tag="VM910_16180"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVI64103.1"
                     /translation="LNMWYDADIDAVMRRTSARPIPQGRIAPGEALAFGIVLAAFAVG
                     VLGLLVSWLAAALLAFTIFFYVAVYTAWLKRWTPQNIVIGGAAGAFPPVIGWVAATGS
                     ISLEPCLLFLIIFCWTPPHFWSLSLVRADDYARARVPMLPVVAGLEETRRQILLYSIG
                     LVPIGAAPWLFGYAHTIYGVTALLAGALMVTFAWRVRCEREGERAERAAYDLFAFSIL
                     YLFVLFAVLLVEGSVIGR"
     gene            709..882
                     /locus_tag="VM910_16185"
     CDS             709..882
                     /locus_tag="VM910_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoxF protein"
                     /protein_id="HVI64104.1"
                     /translation="MRLADMEEKPKRIVLTDEQKRRRRARSIAIALALGALVVLFYVV
                     TLVKGPAVLNRPL"
     gene            888..1487
                     /locus_tag="VM910_16190"
     CDS             888..1487
                     /locus_tag="VM910_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVI64105.1"
                     /translation="MNVRNIPSQRRRDVMVAAACGVFVAAMVGAAYAAVPLYNWFCRT
                     TGFGGTTQVATAAPSHVLDRKLKVRFDANVAGGLPWRFEPDQNSVEVKIGEVVTVSYT
                     VVNESARETVGVASYNVSPSTAGAYFSKINCFCFTDQRLKAGEKRDMTVVFFLAPELV
                     KDSEQDGLDTITLSYTMYPARQAETPQVDTGAASAPGRS"
     gene            1547..2410
                     /locus_tag="VM910_16195"
     CDS             1547..2410
                     /locus_tag="VM910_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVI64106.1"
                     /translation="MAEAHAKPHHDYHLVAPSPWPAIGAVSAFIAAVGGITWMHHMFA
                     AAPYIFGVGAIGIAYTMIGWWTDVAREATYEGYHTRIVQISHRYGMILFIASEVMFFV
                     AWFWAYFNSALFPADMHQLTREQLFGGMWPPKGTETFDPWHLPLLNTLILLTSGTTVT
                     WAHHALLENDRQGLKWGLILTIVLGLAFTCVQAYEYSHAGFNFSGNIYGATFFMATGF
                     HGAHVIIGSTFLIVCLLRAYLGHFTPTQHLGFEFAAWYWHFVDVVWIFLFSCIYVWGS
                     GGASVAHAAGH"
     gene            2721..>2885
                     /locus_tag="VM910_16200"
     CDS             2721..>2885
                     /locus_tag="VM910_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003594578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI64107.1"
                     /translation="MADYDAIIIGTGQAGPALARRLVAAGWKVAIIERKFFGGTCVNN
                     GCTPTKTLIAS"
BASE COUNT          477 a          882 c          899 g          627 t
ORIGIN      
        1 gctcaacatg tggtacgacg cagacatcga cgcggtgatg aggcgcacct cggctcgtcc
       61 cattccgcag ggccgcatcg cgcccggcga ggcgcttgcg ttcggcatcg tgctcgccgc
      121 cttcgcggtg ggggtgctcg gactgctcgt gagttggctc gcggcggcgc tgctcgcctt
      181 caccattttc ttttacgtcg cggtctatac ggcatggctc aagcggtgga cgccgcagaa
      241 catcgttatc ggcggcgccg caggggcctt tccgcccgtc atcggctggg tggcagcgac
      301 cggcagcatc tcgctcgagc cgtgtcttct cttcctcatc atattctgct ggacgccgcc
      361 acatttctgg tcgttatcgc tcgttcgggc cgacgactac gcccgcgctc gcgttccgat
      421 gctcccggtc gttgccgggc tggaggaaac gcggcgacag attctgctct attccatcgg
      481 gctcgtgccg atcggtgcgg ccccgtggct tttcggctat gcccacacaa tttacggggt
      541 gacggcgctc ctcgccggcg cgctgatggt cactttcgct tggcgcgtgc gttgcgagcg
      601 ggaaggcgag cgcgccgaac gtgcggcgta cgatctcttc gctttctcaa tcctctatct
      661 attcgtcctg ttcgcggtgt tgctggtcga gggcagcgtg ataggccgat gaggcttgcc
      721 gacatggaag aaaagccaaa gagaatcgtc ctcaccgatg agcagaagcg caggcggcgc
      781 gcgcggtcga tcgccattgc gctcgcgctc ggtgcgctcg tcgtcctgtt ttacgtggtc
      841 accctggtga agggaccggc ggtattgaat cgtccgctgt gattgcgatg aacgttcgga
      901 acatcccctc gcaacgacgc cgcgatgtca tggtcgcggc cgcgtgcgga gtgttcgttg
      961 cagcgatggt gggcgcggcc tatgcggccg ttccactcta caactggttc tgccgcacga
     1021 ccggcttcgg cggcaccacc caggtcgcga ccgccgcgcc ttcccacgtt ctcgaccgca
     1081 agctcaaggt gcgcttcgat gccaacgtcg ccggtgggct cccctggcgt ttcgaaccgg
     1141 accaaaattc ggtagaggtc aaaatcggtg aggtcgtaac cgtcagctac acggtcgtca
     1201 atgaatcggc gcgggagacc gtgggtgttg cctcctacaa cgtttcaccg tccaccgctg
     1261 gcgcgtattt ctccaagatc aactgcttct gttttaccga tcagcggctc aaggccggcg
     1321 aaaagcgcga catgacagtc gtcttcttcc tggcccccga gttggtgaaa gattccgagc
     1381 aggacggtct cgacaccatt acgctgtcat acaccatgta tccggcgcgg caggctgaga
     1441 cgccgcaagt agacaccggc gcggcgtccg cgcccgggcg aagctgaccc aacaggaacg
     1501 agtgtcgtgg gcagcgctgc ctcacagaga gatggagaag tctaagatgg ccgaagctca
     1561 cgccaagccg catcacgact accatctagt ggcaccgagc ccttggcccg cgatcggggc
     1621 agtttccgcc ttcatcgcgg ccgtcggcgg cattacgtgg atgcaccaca tgttcgcggc
     1681 cgcgccatat attttcggcg ttggtgcgat cggcatcgcc tataccatga tcggctggtg
     1741 gaccgacgtc gcccgtgaag cgacgtacga gggttatcat acgcggatcg tgcagatctc
     1801 ccatcgctac ggcatgattc tgttcatcgc ctccgaggtg atgttcttcg tggcgtggtt
     1861 ctgggcgtat ttcaacagcg cgctgttccc ggccgacatg caccagctga cgcgtgaaca
     1921 gctgtttggc gggatgtggc cgccgaaggg gaccgagacc ttcgacccgt ggcacttgcc
     1981 gctcctcaat acgctgatcc tgctcacctc cggcacgacc gtgacctggg cgcaccacgc
     2041 gcttctggag aacgaccgcc aggggctcaa gtggggtctg atcctgacga tcgtgcttgg
     2101 tctggcattc acttgcgtgc aggcctacga atacagccat gcggggttca atttcagtgg
     2161 caacatctac ggcgccactt tcttcatggc gacgggcttt catggcgcgc atgtcatcat
     2221 cggctcgacc ttcctgattg tgtgcctgct tcgtgcctat ctcggacatt tcacgccgac
     2281 gcagcatctc ggcttcgagt tcgccgcctg gtattggcac ttcgtcgacg tcgtctggat
     2341 ctttctgttt tcctgcattt atgtgtgggg ttcgggcggg gcgagcgtcg cgcatgctgc
     2401 cggccactag agtgcggtct tgatgccttg aatggctcta ccaatttttg gcttgcccga
     2461 atctgaagta gtacctgcga agtcgggttc gagttgactt ggctggcgac cagtcagcct
     2521 tggaagggcg ccggcggcga agcgcaagct gaccgccgtt gcgcggtgat cgcgcaaagc
     2581 cattgctcag tagagcggca ttaccgatcc catatcattg ccgagttgtg aatgctcttg
     2641 tgggcgacag gctggcgatg tccacctatg ctagagatcc tggcttcgtg tgacgacggc
     2701 cacgaaatga gaggcgggtt atggcggact atgatgcgat cataatcggc accggacagg
     2761 ccggaccggc cttggcgagg cgactggtgg ctgccggctg gaaggttgcc atcatcgagc
     2821 gcaagttctt cggcggcacc tgcgtcaata acggctgcac cccgaccaag acgctaattg
     2881 cgagc
//
LOCUS       DATLJT010000709         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_503915,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000709 DATLJT010000000
VERSION     DATLJT010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503915"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VM910_16205"
     CDS             <1..708
                     /locus_tag="VM910_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64108.1"
                     /translation="KESIVGRLKPLGWRGHPLGTDELGRDMLSRLLFGGRVSLLMGVV
                     PVLIASLVGGMLGVLGGFVGGRTNTAIMRTMDVFYAFPSVLLAVAIAGAMGGGMANGM
                     LALTLVFIPPMCRVAETATTQVRALDFIEAARASGARTMTIIRYHVLGNVLGPVFIYA
                     SSLVSVSILLAAGLSFLGLGVRPPVADWGLMLSTLRQSIYVVPLVCALPGLAIFITSI
                     CCNLVSDGLRAAMDVRM"
     gene            712..1758
                     /locus_tag="VM910_16210"
     CDS             712..1758
                     /locus_tag="VM910_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HVI64109.1"
                     /translation="MRPLLHDGLASDPRDRGGPLQPLLIARELRKHFPVKNSGGKSVK
                     AVDGVSFTVRKGETLGIVGESGCGKSTLARLLLHLVVPDAGELVFDGDLVDTRQLPVN
                     ALRRQVQIVFQDSYSSLNPRMPIRDSVAFGPFVQGKKKQQARDIACEMLGKVGLDPDL
                     FGPRYPHELSGGQKQRVNIARALATGPRMVILDEPVSALDKSVEAQVLNLLRALKRQF
                     NLTYLFISHDLNVVRYLSDRVLVMYLGRAVEIGPIDAVFEQPLHPYTRALLASRPSMD
                     PARRIEEAPITGDPPNPIDPPPGCRFHTRCPFTETPCEKTDPALGIWEGPQSHVVACH
                     MHDLASGHSRFENV"
     gene            1751..>2645
                     /locus_tag="VM910_16215"
     CDS             1751..>2645
                     /locus_tag="VM910_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64110.1"
                     /translation="MSEPAANGTDPLAEVNDLSVRFVTREATVHAVNGVSFLVRPGEV
                     LCILGESGSGKTVTLRALMRLLPPRRTQIEGRVRVEGHDILAMRPRALRDLRGGLVSM
                     VFQEPMTALDPVYTVGQQIGETLRRHTGCDRRAARTRALELLELVRIPSAERRLDAYP
                     HELSGGLRQRAMIAMALSCNPRLLLADEPTTALDATVQVQVLVLLRRLQRELGMGMVF
                     VTHDIGVAAEIADTVAVMYAGRIVESGPVAQVLKAPAHPYTAGLLASTVHGQPRERDI
                     CAIPGSPPDLRRLPPGCSFAPR"
BASE COUNT          394 a          834 c          900 g          517 t
ORIGIN      
        1 aaggaaagca tcgtcgggcg gctgaagccg cttggttggc gcggtcatcc gctcggcacc
       61 gacgagctcg ggcgcgatat gctgtcccgg ctgctttttg gtggccgggt atcattgctg
      121 atgggcgtcg tgccggtgct gatcgcttcg ctcgtcggcg gcatgctggg tgtgctcggc
      181 ggatttgtcg gcgggcggac caacaccgcc atcatgcgca cgatggacgt gttctacgcg
      241 tttccgtcgg tgctattggc ggtcgcgatc gccggtgcga tgggcggggg catggccaac
      301 ggcatgctgg cgctgacctt ggtgttcatc ccgccgatgt gccgtgtggc ggaaaccgcg
      361 acgacccagg tgcgggcatt ggattttatc gaggcggccc gcgccagcgg agcgcgcacg
      421 atgaccatca tccgctacca cgtgctcggc aacgtgctgg gaccggtgtt catctacgcc
      481 tcctccttgg tcagcgtcag cattctgttg gccgcgggac tgtctttcct cggcctcggc
      541 gtgcggccgc cagtggcgga ttgggggttg atgctgagca ccttgcgcca gtcgatctat
      601 gtcgtgccgt tggtctgcgc gctaccgggt ctggcaatct tcatcacctc gatctgctgc
      661 aacctggtca gcgacggact gcgcgcggcg atggacgtgc ggatgtaggc cgtgcgcccg
      721 cttttgcatg atggtctggc atcggatccg cgggatcgcg gtggcccgct tcagccgctg
      781 ctgatcgccc gcgagctgcg caagcatttc ccggtcaaga acagcggcgg caagtcggta
      841 aaggcggtcg acggcgtgtc gttcacggtg cgcaagggcg agacgttggg gattgtcggc
      901 gaatcgggct gcggcaagtc gaccctcgcc cggctgttgt tgcatctggt ggtgccggat
      961 gccggcgagc tggtgttcga cggcgatctg gtcgacacgc gccagctccc ggtgaatgcg
     1021 ctgcgccgcc aagtacagat cgtgtttcaa gattcctact cctcgctcaa tccgcgtatg
     1081 ccgattcgcg actcggtggc cttcggcccg ttcgtacagg gcaagaaaaa gcagcaggcg
     1141 cgggatatcg catgcgagat gctcggcaag gtgggcctcg atcccgacct gttcggcccg
     1201 cgttatccgc acgagttgtc gggcggccag aagcagcggg tcaacattgc tcgcgcgctt
     1261 gccaccgggc cgcgcatggt gattctcgac gaaccggtct ccgcgctcga caaatcggtc
     1321 gaagcgcagg tgttgaacct gttgcgcgca ctgaaacggc aattcaacct gacctatctc
     1381 ttcatcagcc atgatttgaa cgtggtccgc tacctcagcg accgggtctt ggtgatgtat
     1441 ctcgggcgtg cggtcgagat tggcccgatc gatgcggtct tcgagcagcc gcttcatccc
     1501 tatacgcgcg ccctcttggc gtcgcgcccg tcgatggacc cggcccggcg gatcgaggag
     1561 gcgccgatca ccggcgatcc gccgaacccg atcgatccgc ccccgggctg tcgtttccat
     1621 acccgttgcc cgttcaccga gacgccgtgc gagaagacgg atccggcgct tggcatctgg
     1681 gaaggaccgc aatcgcacgt cgtggcctgc cacatgcacg acttggcctc gggtcacagc
     1741 cggtttgaga atgtctgagc ccgcagcaaa cggcaccgac ccgctggccg aggtgaatga
     1801 tctgagcgtg cgtttcgtga ctcgcgaagc caccgtgcac gcagtgaacg gggtgagttt
     1861 ccttgtgcga cccggcgagg tgctgtgcat ccttggcgaa tccggctccg gcaagaccgt
     1921 gaccttgcgg gcattgatgc gcctgttgcc gccgcggcgc acgcagatcg aggggagggt
     1981 gcgcgtggag ggtcacgata tcctggcgat gcgaccacgc gctctgcgcg atctgcgtgg
     2041 cggcctggtg tcgatggtct tccaagagcc gatgacggcg ctcgacccgg tctacacggt
     2101 cggccagcag atcggcgaga cattgcgccg ccacaccggc tgtgaccggc gcgcggcgcg
     2161 gacgcgggca ttggaattgc tggaattggt gcggatcccg tcggccgagc ggcggctcga
     2221 cgcctatccg cacgaattgt cgggcggatt gcgccagcgc gcgatgatcg caatggcgtt
     2281 gtcgtgcaac ccgcgcctgc tgttggccga cgagccgacg accgcgctcg atgccacagt
     2341 gcaggtccag gttctggtac tgctgcgtcg actacagcgc gagctcggca tgggcatggt
     2401 cttcgtcacc catgatatcg gcgtcgctgc cgagatcgcc gacactgttg ctgtgatgta
     2461 tgccggccgc attgtcgaaa gcgggcctgt cgcgcaagtg ctaaaggcgc cggcgcatcc
     2521 ctataccgca gggctgctgg cttcgaccgt gcacggtcag ccgcgcgagc gcgacatctg
     2581 cgcgatcccc ggcagcccgc ccgacctgcg ccgcctcccg ccgggctgca gcttcgcgcc
     2641 tcgct
//
LOCUS       DATLJT010000710         2948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_962094,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000710 DATLJT010000000
VERSION     DATLJT010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962094"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..1768
                     /gene="oxlT"
                     /locus_tag="VM910_16220"
     CDS             479..1768
                     /gene="oxlT"
                     /locus_tag="VM910_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HVI64111.1"
                     /translation="MEETVTTSTTEAISSRATASEATRWGQLIFGIICMVMIANLQYG
                     WTLFVTPIDQKYHWGRAAIQVAFTIFVLTETWLVPFEGYLIDKFGPRIMISGSGVLVA
                     IAWVINSVADSLVLLYIGAAIGGIGAGVIYGGSVGNALKWFPDRRGLAAGLTAAGFGA
                     GSALTVIPIANMIQSSGYEAAFLWFGLAQGIVVILVALLLRAPEAGEVAIPAAPAVLQ
                     TRRDYGPAEVLKTPVFYVMYAMFVMVGAGGLMAIAQLAPIANDYKIAGVPVSILGLTL
                     PALTFALTIDRVLNGICRPFFGWVSDHIGRENTMFIAFMLEGIGIYALLYFASNPLLF
                     VILSGVVFFAWGEIYSLFPATCTDIYGRKFATTNYGMLYTAKGTASLLVPLANVLTSA
                     TGSWHAVFYVAAILNIIAAVMALVVLRPMRLRAVEKG"
     gene            complement(1870..2028)
                     /locus_tag="VM910_16225"
     CDS             complement(1870..2028)
                     /locus_tag="VM910_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64112.1"
                     /translation="MSDLAARFVYSPLLSTAFKVVCLFCVLGLALSVAIVPMIAPEHL
                     AWVLAHIE"
     gene            2278..>2948
                     /locus_tag="VM910_16230"
     CDS             2278..>2948
                     /locus_tag="VM910_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI64113.1"
                     /translation="MSPGVRNPRRASVRGPSAPRPAGAAISPRVEPIGSPDSFKRRAY
                     VALKDAIASMDIYRSRTDIRLDERRLASDFGISRTPVREAMAQLEREGFVRAVPRRGI
                     YVVRKTKREVIEMITAWAALESMAARLITDNASADEIASLRTMFAKFENGELHARLDE
                     YSEVNIEFHQTIIRMSRNGVLISLAENLFTHMRMIRRKTIGEQDRADRSIRDHLSIIE
                     ALEAR"
BASE COUNT          543 a          904 c          938 g          563 t
ORIGIN      
        1 acgctgccgg aacagctgaa atagatccgg cacgagcgcg gtgcatcgcg gcccgccggc
       61 gggtgagcca acgcaatcgg cgtcaagcgc gcctgcacaa ccgaatattg cgccgcacac
      121 aagggggtgc ggcaccacgt cccggccccc ccagcgcaat tgcgggcatt gtcccttagg
      181 gtaggctggt tttattcaaa cctgcgtgta atttaccaag gatggaggcg acgaaacgaa
      241 ctttattgtg ggttgtcggg tttgttgctt catagggatc gacgttgtca gcgggcgaac
      301 gaggacgtgt cagggaacga cgcaacgcgc gcaaggagat ttcatttcac cggacacgcg
      361 aggtgaaatc gaacgcggaa aaaacgtgtc gagcgccgcg gtaagcaagc gtgctcataa
      421 ataagggtcg gcggcgaggc gcgacgtctc gtccgagagt tcgactaata cggttgggat
      481 ggaggaaacg gtgaccacaa gcacaacaga agccatttca tcgcgggcca cggcgtccga
      541 agcgacgcgt tggggccaac tgatcttcgg catcatctgc atggtgatga tcgccaacct
      601 gcaatacggc tggacgctgt tcgtcactcc gatcgatcag aagtatcact ggggacgcgc
      661 ggcgatccaa gtcgcgttca ccatcttcgt cctcaccgaa acctggttgg tgccgttcga
      721 gggctatctg atcgataagt tcggcccgcg gatcatgatt agcggcagcg gggtgttggt
      781 cgcgatcgcc tgggtcatca actcggtggc cgactcgctc gtcctcctct acattggcgc
      841 ggcgatcggc ggcattggcg ccggcgtaat ctacggcggc tcggtcggca atgcgctgaa
      901 atggttcccg gatcggcgcg gcctcgcggc tggcttgacc gccgccggtt tcggcgctgg
      961 ttccgctctc accgtaatcc cgatcgccaa tatgattcag tcgagcggtt acgaggctgc
     1021 gttcctgtgg ttcggtcttg cacagggcat cgtggtgatc cttgttgcgc tgctgctgcg
     1081 ggcgccggag gccggcgagg tcgcgatacc cgcagcgccc gctgtcttgc agacccggcg
     1141 cgattacggc ccggccgaag tgctgaagac gccggtgttc tacgtcatgt acgcgatgtt
     1201 cgtgatggtc ggcgccggtg gtctgatggc aatcgcccag cttgcgccca ttgccaacga
     1261 ctacaaaatc gcgggtgtgc cagtctcgat cctgggactt acgctcccgg cgcttacctt
     1321 cgccttgacg atcgaccggg tgctcaacgg catctgtcgg ccctttttcg gctgggtgtc
     1381 cgatcatatc ggtcgcgaga acacgatgtt catcgccttc atgttggaag gcatcggcat
     1441 ttatgcgttg ctctacttcg cgagcaatcc actcctgttc gtaatcctgt cgggggtggt
     1501 gttcttcgcc tggggcgaga tctactccct cttccccgcg acctgcaccg acatttacgg
     1561 caggaagttc gcgacgacga attacggcat gctttatacg gcgaagggga cggcatcgct
     1621 gctggtcccc ttggccaatg tgttgaccag tgcgaccggc agttggcacg ccgtatttta
     1681 tgtcgcggcg atcctcaaca ttatcgcggc ggtcatggcg ctcgtcgtgt tgcggccgat
     1741 gcgactccgc gccgtggaaa agggttgacg gtgccgggaa acgaggactg tctgcgccgt
     1801 tgacggcggg gacggtcctc gaactcatgc cgtcggcgcg gcgaatgacg ccatgaaaat
     1861 ggaaacgtgt cattcgatgt gggccagcac ccaagccaga tgctccggtg cgatcatcgg
     1921 aacgatcgct accgagagcg ccaagcccaa cacgcagaac aagcacacga ccttgaacgc
     1981 ggtcgaaagg agcggcgaat acacaaagcg agcggcaaga tcggacatcg gttcctcctc
     2041 ggcatcgcac cgatgtcgta gcgttcccgg cgcgcgagcc gtgtgaaccg gctcacctgc
     2101 ccggcgcttt tatagctgct gccgctcggc cttgaccgca ggttcgcgac cgcccggggg
     2161 gtcagcgatg cgggcaacgg atgccggggc ggcgccgccc gctttgcgcg aagcctggat
     2221 gggggatgaa aggtgccggt tgcatcgatt catgccgtgg aatattgtat accagccatg
     2281 agtcctggtg tccgtaatcc tcgtcgcgcc agcgtccggg gcccatccgc gccccgcccc
     2341 gcaggtgccg cgatatcgcc gcgcgtcgag cccatcggca gcccggacag cttcaagcgc
     2401 cgcgcctatg tcgcgctcaa agacgcgatc gcgtccatgg acatctatcg cagccggacc
     2461 gacatccggc tcgacgagcg gcggctcgct tcggatttcg gcatcagccg cacgccggtg
     2521 cgcgaagcca tggcgcagct cgagcgtgaa ggtttcgtgc gcgcggttcc ccggcgcggg
     2581 atttacgtcg ttcgcaagac caagcgcgag gtgatcgaga tgatcaccgc ctgggccgca
     2641 ctcgagagca tggccgcgcg gctcatcacc gacaacgcca gcgccgacga gatcgcttcg
     2701 ctgcgcacca tgttcgcgaa gttcgagaac ggcgagctgc acgcccggct cgacgaatat
     2761 tccgaggtca acatcgagtt ccaccagacg attatccgca tgagccgcaa cggcgtgctc
     2821 atctcgctcg cggagaacct gttcacccat atgcggatga tccggcgcaa gaccatcggc
     2881 gagcaggacc gcgccgaccg ctcgatccgc gaccatctga gcatcatcga ggcgctcgag
     2941 gcgcgcga
//
LOCUS       DATLJT010000711         7141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2111626,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000711 DATLJT010000000
VERSION     DATLJT010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111626"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VM910_16235"
     CDS             <1..342
                     /locus_tag="VM910_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HVI64114.1"
                     /translation="SEEERAQFSGIAAGMYRASDTDDDISFMRLDQQFNTLVSLAARN
                     EFASRSMGLMHGLSRRFWYQHYKQAADLPLCARLHAAVAEAIANRDPDKAAEASDRLI
                     DYIESFARSTL"
     gene            complement(418..1527)
                     /locus_tag="VM910_16240"
     CDS             complement(418..1527)
                     /locus_tag="VM910_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HVI64115.1"
                     /translation="MTLKYRAAVLHQAGKPLAIEQVSSGDPAPTDVVVRVRAAGLCHT
                     DLEVIDGSLRYPLPMILGHEAAGVVEQIGPEVDTVKVGDHVILSWNPHCGHCFQCDRG
                     QPILCDTYLGEGPKGLHFDGRSKASLGDGAELRHLMFLGAFAEICTVPAQQAIVVPKE
                     IPFDRACLIGCGVMTGVGAALNVADIAYGDTVMVIGCGAVGLAALQGAVMARAGAVIA
                     VDLDERKLGLASKLGARHTVNAKRQDPVEFVRSVTGGRGADVVLEAAGHPAAFRQTAE
                     AVRVGGQVIWLGKIDVANDVSFRWGSLMGEKRLRRSSYGGARPLRDFPLLARAYLEGR
                     LKLDELITARARLDDINRGFADLREGRSIRTVIEF"
     gene            complement(1550..1858)
                     /locus_tag="VM910_16245"
     CDS             complement(1550..1858)
                     /locus_tag="VM910_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HVI64116.1"
                     /translation="MKRYSGERTIDGISVLVDGKPLSPHIDQLRLTDYGFEWSYEGPE
                     PSQLAFAILFDHLGNVAEAKSLYPSFMQRIVANFDNEWEMTSADIDAAIAALRGERAA
                     "
     gene            complement(1880..2068)
                     /locus_tag="VM910_16250"
     CDS             complement(1880..2068)
                     /locus_tag="VM910_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64117.1"
                     /translation="MPFRVKFLLSLAWLLTAAGGYAFMVHLGQTGPSYAVAFLGVFAV
                     FAMWVFPEVTKKNPASLK"
     gene            complement(2085..2483)
                     /locus_tag="VM910_16255"
     CDS             complement(2085..2483)
                     /locus_tag="VM910_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64118.1"
                     /translation="MNHFEPNLVALSWFTLLWSVCCLGFLQLAGMYPLRAGTAGEARN
                     PVFLVLGNTALWLALLAGTLAFASTQLRWTTIVVVAGLLFLFIPELFQAIPARLRDGR
                     KGMTIAGCILAATLGLLAWLSSGTVGGPHA"
     gene            complement(2532..3317)
                     /locus_tag="VM910_16260"
     CDS             complement(2532..3317)
                     /locus_tag="VM910_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVI64119.1"
                     /translation="MELAMTPMTERQRKYRENYRERVAGWYNGWLHILLIYTIGFTAL
                     YVYIANLHDVRWWEYLAIPVVFMFANFFEWAVHRYVMHRPSNIAMLRAIYSRHTLMHH
                     QFFTEQEMRFADHHDWRVTFFPPYALVAFTFMSIPPAIFAGWLISPNVGWLLISTTTS
                     MYLIYEFMHFCCHVDDNWFVRHMPFVNTIRRHHTAHHNQSIMMERNMNLTFPIMDCLM
                     GTSDLNRGVLGHVFNGYDTRYVKTDMRKTARTPRVMPSAVPAE"
     gene            complement(3454..5244)
                     /locus_tag="VM910_16265"
     CDS             complement(3454..5244)
                     /locus_tag="VM910_16265"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HVI64120.1"
                     /translation="MSLAGELQRKPTTTFDLPPWLGPVGLAVAIGLVYFVAARLSLAL
                     LTKPEGVAVFWPAAGVSAGALIAFGPRARWPVVAGTMVATIVANLLGDRNLWGAILFA
                     LCNAGEAVLAAWLITRYYGEDFRLNKPRNVVGLVAAAVTGAAVSGIGGVIGFRLFHNS
                     STPILTIWEHWFASDGLGIITVAPVVIELAAAWRDRPPLTELAEGAITVAALALVSAF
                     AIFLRSELLANVGPVAVLFAPLLWLAARCRPVFAAAAALIMSLSIVWTTTFGIGYFGN
                     PDLSMDERVLSAQVSILLVTIGASLLAALFAEFRDKRKVAEAALHASETQRYLIETER
                     LAALGRLVAGVAHELNSPVGISLTVASTLANRCRDFGSAIFSGPVRRSFVAEFNDGCR
                     DAANQLVANLERAGELIQAFKQVAVDRSEADRRSFDLKLATEQIVAAVRPGLPKPRGP
                     LALEIPADITLDSYPGAYGQVLTNLIFNAVTHAFTDGAVGNMQLNARRIGSGQVEITF
                     SDDGRGIPEEVQRHVFDPFFTTRRAKGNTGLGLYIVHNLVTEQLGGRITLVSAPGKGT
                     TICMTLPLVASSHAEKTGAAGRGKLAGGPN"
     gene            5862..6005
                     /locus_tag="VM910_16270"
     CDS             5862..6005
                     /locus_tag="VM910_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64121.1"
                     /translation="MKKFIADRPFADPDAAARKIVEIAKEGEAVQNGRIYIERVNAPF
                     LAV"
     gene            6122..6514
                     /locus_tag="VM910_16275"
     CDS             6122..6514
                     /locus_tag="VM910_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64122.1"
                     /translation="MIDHTGIGVADMMRSARFYDAALGALGLRRAMQLPPGDGSDGVG
                     YGIDYPVFWIDRFHPHSVRQHTAFAAKNRAQVDAFHAAALAAGGTDNGPPGLRDAAVG
                     YPAGYYAAFVLDPDGNNIEAVFRGGTAP"
     gene            6532..6621
                     /locus_tag="VM910_16280"
                     /pseudo
     CDS             6532..6621
                     /locus_tag="VM910_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            6866..7006
                     /locus_tag="VM910_16285"
     CDS             6866..7006
                     /locus_tag="VM910_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64123.1"
                     /translation="MRQSPSIVPDSFDRDIYLALDDFGDRLGWAWPETDVAHTIAANP
                     SR"
BASE COUNT         1389 a         2252 c         2247 g         1253 t
ORIGIN      
        1 agcgaggaag agcgcgcgca gttttccggg attgcggccg gcatgtatcg cgcctccgat
       61 acggacgacg acatctcctt catgcggctc gaccagcagt tcaacacgct ggtttcgctc
      121 gccgcgcgca acgaatttgc cagccgctcg atgggcctga tgcacggcct gtcgcggcgc
      181 ttctggtacc agcactacaa gcaggccgcc gacctgccgc tctgcgcgcg gctgcatgct
      241 gcggttgccg aggcgatcgc aaaccgcgat ccggacaagg ccgccgaagc ctccgaccgg
      301 ctgatcgact atatcgaaag ctttgcccgc tcgacgctgt gagcgcgtcc gctcgattta
      361 tctgcgcgtt cgctttacct ctccccgtcg atgagaaggt aaacgcccca tcctgcacta
      421 gaactcgatc acggtgcgga tcgaacgccc ctcgcgcagg tcggcgaaac cgcgattgat
      481 gtcgtcgagc ctcgcgcgtg cggtgatcag ctcgtcaagc ttcaggcggc cctcgagata
      541 agcgcgcgcc agcagcggaa agtcgcgcag cggccgcgcg ccgccatagc tcgaccgccg
      601 caggcgcttc tcgcccatca gcgaacccca gcggaagcta acgtcgttgg cgacatcgat
      661 cttgccgagc catatcacct ggccgccgac gcgcacggcc tccgcggtct ggcggaaggc
      721 agccggatgg cctgccgcct ccagcaccac gtctgcgcca cgtccgccgg tcacggatcg
      781 cacaaattca acggggtcct ggcgtttggc gttcaccgtg tgccgcgccc cgagcttgct
      841 cgcaagccca agcttccgct catccagatc gaccgcaatc accgcgccgg cgcgcgccat
      901 gaccgcgccc tgcagcgccg caagccccac cgcgccgcaa ccaataacca tcacggtgtc
      961 gccataggcg atgtcggcga cattgagcgc ggcgccgacg cccgtcatca caccgcaacc
     1021 aatcagacag gcgcggtcga acgggatctc cttgggcacg acgatcgcct gctgcgccgg
     1081 cacagtgcag atttcggcaa aggcgccgag gaacatcagg tgcctcagtt cggctccgtc
     1141 gccgaggctc gccttcgagc ggccatcgaa gtgcagtccc ttcggtccct cgcccagata
     1201 ggtatcgcac aggatcggct ggccgcgatc gcactggaag caatgaccgc aatgcggatt
     1261 ccacgacagg atcacatgat cgccgacctt gaccgtatcg acctcggggc cgatctgctc
     1321 gacgacgccg gccgcctcat ggccgaggat catcggcagc ggatagcgca gcgagccgtc
     1381 gatcacctcg agatcggtgt ggcaaaggcc ggcggcgcgg accctgacga ccacatccgt
     1441 cggcgcggga tcgccggatg acacctgctc gatcgcgagc ggctttccgg cctggtgcag
     1501 cacggccgcg cggtatttga gcgtcatgga aaggcttccg gaaagcgagc taggcggcac
     1561 gctcgccgcg cagcgcggcg atcgcggcgt cgatgtcggc gctcgtcatt tcccactcgt
     1621 tgtcgaaatt ggcgacgatc cgctgcatga agctcggata gaggctcttt gcctccgcga
     1681 cgttgccgag atgatcgaac aggatcgcga aggcaagctg cgaaggctcg gggccttcat
     1741 agctccactc gaagccataa tcggtgagcc tcagttggtc gatgtgcggc gacagcggct
     1801 tgccgtcgac cagcacggag atgccgtcga tggtgcgctc tccggagtat ctcttcatcg
     1861 tcttctcctg cctgccaccc tacttcagcg aggccggatt cttcttggtg acttccggaa
     1921 acacccacat cgcaaacact gcgaagacgc cgagaaaggc aactgcgtag ctcggcccgg
     1981 tctgtccgag atgaaccatg aaggcatagc cgccggccgc cgtcaaaagc cacgccagcg
     2041 acagcaggaa cttgacacga aacggcatcc tttcctccta tgtctcacgc atgcggtccg
     2101 ccgaccgttc cggacgaaag ccaggccaga agaccaagcg tggcggcgag aatgcagcct
     2161 gcgatcgtca ttcccttgcg tccgtcccgc agacgggccg gaatcgcctg gaacaattcg
     2221 gggatgaaca ggaacaacag acctgcgacg accacgatcg tcgtccagcg caactgcgtc
     2281 gaggcgaaag caagcgtgcc ggcaagcagc gccagccaca atgcggtgtt gccgagcacc
     2341 agaaaaacgg gattgcgcgc ttcgccggcg gtgcctgcgc gcagcggata catccctgcg
     2401 agctggagaa agccgaggca gcacacgctc cagagcagcg tgaaccagga cagcgcaacc
     2461 agattgggct cgaaatggtt caccggcgaa actccaaacc agatgaaatg gccgcgggcg
     2521 cggttcgcgt cctactccgc gggcaccgcc gagggcatca cgcgcggcgt ccgcgcggtc
     2581 ttgcgcatgt cggttttgac ataacgcgtg tcgtagccgt tgaaaacatg cccgagcacc
     2641 ccgcgattga gatcggacgt gcccatcagg caatccatga tcgggaacgt cagattcatg
     2701 ttgcgttcca tcatgatcga ctggttgtga tgtgcagtat gatgacggcg gatggtgttg
     2761 acgaacggca tgtggcgcac gaaccagttg tcgtcgacat gacagcagaa gtgcatgaac
     2821 tcgtagatca gatacatcga ggtcgtggtc gagatcagaa gccagccgac attcggcgag
     2881 atcagccagc cggcgaaaat cgccggcgga atcgacatga aggtgaacgc cacgagcgcg
     2941 taaggcggaa agaaggtaac gcgccaatcg tggtgatcgg cgaagcgcat ctcctgttcg
     3001 gtgaagaact ggtggtgcat cagggtgtgg cgactataga tcgcgcgcaa catcgcgatg
     3061 ttggacggac ggtgcatcac gtagcggtgc acggcccatt cgaagaaatt ggcgaacatg
     3121 aacacgacgg ggatggcgag atattcccac cagcgcacgt cgtggagatt ggcgatgtag
     3181 acgtacagcg cggtgaagcc gatggtgtag atcagcagga tgtggagcca gccgttgtac
     3241 cagccagcga cgcgctcgcg gtagttctcc cggtatttgc gctgccgttc cgtcatcggc
     3301 gtcattgcca gttccatggc agttctcccc gggcgtttcc tcaaaactga catatcagta
     3361 gcacatcaga tgccgaaggc aaggctggaa gctttggttc cggtagcatt tgcgcaaacc
     3421 cgtagcacta cggatgcatt ccccaaaacg gcatcaattg gggccgcccg cgagcttgcc
     3481 gcgcccggcc gcacccgtct tctccgcgtg gctggaggcg accaggggca gcgtcatgca
     3541 gatggtcgtg cccttgccgg gtgccgagac cagcgtgatc cggccgccga gctgctcggt
     3601 gacgagatta tgcacgatgt agaggccaag ccccgtgttg cctttggcgc ggcgcgtggt
     3661 gaagaacggg tcgaacacgt gacgctgaac ttcctcggga atgccgcgcc cgtcgtcgga
     3721 gaacgtgatc tcgacctggc cggaaccgat gcgtcgcgcg ttgagctgca tattcccgac
     3781 agccccgtcg gtgaaagcgt gggtgacggc attgaagatg aggttggtca gcacctggcc
     3841 gtaagcgccc gggtagctgt cgagcgtgat gtcggcgggg atctcgagcg cgagcggacc
     3901 cctgggcttc ggcaggccgg gccgcacggc agcgacgatc tgctccgtcg cgagcttgag
     3961 gtcgaaactg cgccggtccg cctcgctgcg atcgacggcg acctgcttga acgcctgaat
     4021 cagttctccc gcgcgctcga gattggcgac gagctgattg gcggcatcgc ggcaaccgtc
     4081 gttgaactcg gcaacgaagg aacgacgcac gggtcccgag aagatggcgc tgccgaagtc
     4141 cctgcaacgg ttcgcgagcg tggaggccac cgtgaggctg atgccgaccg ggctgttgag
     4201 ctcgtgcgca acgccagcaa cgagacggcc gagtgcagcg aggcgctcgg tttcgatcag
     4261 atagcgttgc gtttcgcttg cgtgcagggc ggcttcggcc accttcctct tgtcacggaa
     4321 ttcggcaaac agcgcggcaa gaagcgatgc gccgatggtg acgagcagaa tgctgacctg
     4381 ggccgaaagc acgcgctcgt ccattgaaag gtccggattg ccgaaatagc cgatgccgaa
     4441 ggtggtggtc cagacgatcg acaggctcat gatgagcgcg gcggcggcag cgaaaaccgg
     4501 tcggcaccgc gccgcgagcc acaatagcgg cgcaaacagc acggcgaccg gcccgacatt
     4561 cgccagaagc tccgaccgca gaaagatggc aaaggcactc acgagcgcca gtgcggccac
     4621 cgttattgca ccttcggcga gctcggtcag cggtggccgg tcgcgccagg cagcggccaa
     4681 ctcgatcacg actggcgcga ccgtgatgat ccccaatccg tcggatgcga accagtgctc
     4741 ccagatggtc aatatcggcg tcgacgagtt gtgaaacaat ctgaacccga tgacgccgcc
     4801 gatcccggag acggcggccc ctgtcacggc cgccgcaacc aaccccacta cattgcgcgg
     4861 cttgttcagc ctgaagtcct cgccatagta acgcgtgatc agccaggccg cgagcacggc
     4921 ctcccccgcg ttacataaag cgaagagaat ggcgccccag agattccggt cgccgagcag
     4981 attggccacg atcgttgcca ccatcgtgcc ggcaacgacg ggccatcgcg cgcgggggcc
     5041 gaatgcgatc agagcgcccg ccgacacgcc cgcggcaggc cagaacaccg ctacaccctc
     5101 gggcttggtc agcaacgcaa ggctcagccg cgccgccacg aaatagacca ggccgatcgc
     5161 gacagcgagc ccgaccgggc ccagccatgg cggcaggtcg aacgtcgtgg tcggtttacg
     5221 ctgaagctcc cctgctaggc tcacccggta ctccttcccc gcaccttcac gtttagcaca
     5281 aggcggcacg caagccgacc accctaaggc ggcgcgcggg ccccctaatt ccgtgtgagg
     5341 agcggagcct agcaatccca ggcatttggt ggggctttcg cctgcacagc taccacatag
     5401 ctaccacccg gaaccctcac tcgacatcgg gcaaatttat gtcgggccag cgctgcaaaa
     5461 agctcgctga ggaatgggtc gctattcttt acgcttcacg gttggaagcg tctttataag
     5521 ttcccaagta ctggtgcaga tagccaatca tctcgacagg agcatcggac ttgaattgat
     5581 gccgcttatg tctccctgta ccaagacaag tagaggtagt cggtcgccgc ttccacattg
     5641 cgctgtattg gttcgagcga tagaggatca agcgcgccag cgagggtttt cgcgacttta
     5701 cctttacact tcggatgctg tgggcttcta cgagaggctc ggctgggaat tgttagaccg
     5761 cacaaactgg aagggcttcg acacggcact gatggtccgt gatctatgac gccggtcaag
     5821 atcgtgctgc gcggtgcgga taaggaagat cgggtggcag tatgaaaaag ttcattgccg
     5881 atcgaccctt cgccgatccc gatgcagccg ctcgcaagat cgtcgaaatc gccaaggaag
     5941 gcgaggccgt gcagaacggt cggatttaca tcgagcgggt gaatgcgccg tttcttgctg
     6001 tatgaggcac cggcgacgac ttccgcgccg gcgtggaacg cgccatcgct ctcccgcgag
     6061 aaaggtaact tgccgagcga gcagcccccc gcgttcgcgc gacgtatcat aaggagttta
     6121 catgatcgac cacaccggaa tcggcgttgc cgacatgatg cgctcggcgc ggttctacga
     6181 tgcggccttg ggagcgctcg ggctgcgacg ggcgatgcag ttgccgccgg gcgatggttc
     6241 ggacggtgtg ggttatggga tcgattatcc ggtcttctgg atcgaccgct tccatccgca
     6301 cagcgtgcgc caacataccg cgttcgccgc caagaaccgg gctcaagtgg acgctttcca
     6361 cgctgccgct ctcgccgccg ggggtacgga caatggtccg cccggcttac gggacgccgc
     6421 agtgggctat ccggcaggct actacgccgc cttcgtgctc gatccggacg gtaacaacat
     6481 cgaagctgtc ttccggggcg ggacggcccc gtgaaagact actcgcgcgg gatgtgtgac
     6541 ctatattcaa tcaccaccaa ccaagccgcg atcatcgcgt tgttccgcgt catcagcaga
     6601 tacgtcggca acctggcgcc gagttcgatg gtcgagttag tagtggccgt cgtaggtctg
     6661 gcgagcgccg gcatttttct ggcgcatgca gttgcggcct atccaccgcg cggtttgtct
     6721 gccgggcttt tggcaagcgt ggtgcggacg tgcggccgga cttcgattgg aaccgacagc
     6781 cggttggaat gatgggctat cggtagggat tcgcaattcg actgcagggg cggctatctt
     6841 cagcgcctgt tgcggggagt tgcgtatgcg tcagtcgccc tccatcgtgc ccgactcctt
     6901 tgatcgggac atctatctcg cgctcgatga cttcggcgat cggcttggct gggcttggcc
     6961 agagaccgac gtggcccata ccatagccgc caatccgtca cggtgacgcc gcagcccagc
     7021 ggctgattgc gcgggtcggg cccccatcgc tcactggggc atgtcccgac cactagcccc
     7081 gctctccccc aggcagcggg gttttccttt tttgcgtggg gtcggtcgcc cgcaggtgcc
     7141 g
//
LOCUS       DATLJT010000712         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3949677,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000712 DATLJT010000000
VERSION     DATLJT010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949677"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VM910_16290"
     CDS             complement(<1..813)
                     /locus_tag="VM910_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64124.1"
                     /translation="MNSTIMLFLIQDGITNGAIYALLGLALVLVFAVTRVILIPQGEF
                     VTYGALTYASLSSGQMPGTAKLAMAMGAAAFVFDLIAARRALDGKRIARAFAVDILLP
                     GAVLAATLSVVSRHPPIGVSIALSLAVVACIGLFLYRLAFQPLAHTSVLVLLIASVGC
                     HLAMQGLGLLFFGAEGQRGPAVADIALTFGPLRFTGQSLAVYAFTIGFIVALWLFFGF
                     SLYGKALRATAVNRLGARLAGIRTTLSGQIAFLLAALIGALSGILIVPITTLY"
     gene            992..1501
                     /locus_tag="VM910_16295"
     CDS             992..1501
                     /locus_tag="VM910_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI64125.1"
                     /translation="MTVSKTAFADGARTRKEAVDGAGEGSALQLGELSELLGYSLKRA
                     QLRIFEDFLRCVAPLQLTPAQFSVLLLLDKNPGRNQTEIANTLGILRPNFVAMLDGLE
                     SRDLCTRVRSTNDRRSHVLLLTDKGKAVLARAKKLVATKHEARLNELLGAPNRAALLA
                     MLTKIAQEF"
     gene            complement(1532..2812)
                     /locus_tag="VM910_16300"
     CDS             complement(1532..2812)
                     /locus_tag="VM910_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HVI64126.1"
                     /translation="MTDRRPLLRAIYDAAVAAAHPDVVLERHLRPVPKGKVICLAAGK
                     GAAAMASAAERHYLDALELDPARLSGIATTRHGHGVPTRRIRVVEAGHPMPDEAGLKA
                     AEETLRLAQTAGEDDLLLVLLSGGGSANWIAPVDGVTFAQKQKLTRALLRSGAPIGEV
                     NTVRKHLSRIKGGRLARAGQRAEIVTLAISDVPHDDPSAIASGPTVPDPTTLADARAI
                     VAKYGLEVDDAIRRALEDSANESCKPGDPAFARTRLELIARPRASLDAAIRLAKDAGY
                     ETIDLGADLEGEAREVAADHARLALAARAQGKRVAILSGGELTVTVRGNGRGGPNQEY
                     ALALADLLKDTTGIAALAGDTDGADGGAGSATDPAGAMMDAATFAKMRSQRLDPRAYL
                     DNNDATAFFAATGDLLLTGPTLTNVNDIRVILVE"
     gene            complement(2932..>3286)
                     /locus_tag="VM910_16305"
     CDS             complement(2932..>3286)
                     /locus_tag="VM910_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI64127.1"
                     /translation="DVSDPQWDNDAGMKQFLDFLAKDFPEGNKLDASVVVGYGVAQTL
                     VQVLKQCGDNLTRENIMRQAANLKDFRTEVLLPGVMINTSPSDFAPISQLQLERFKGE
                     RWELFGDVVSGEVGG"
BASE COUNT          604 a         1065 c         1083 g          534 t
ORIGIN      
        1 atagagggtc gtgatcggca cgatcaggat gcctgacagc gccccgatca gcgcggccag
       61 caggaaggcg atctggcccg acaacgtggt cctgatgccg gcgagccgcg ccccgagccg
      121 gttgacggcg gtcgcgcgca acgccttgcc gtacagcgag aacccgaaga acagccagag
      181 cgcaacgatg aagccgatgg tgaaggcata gacggcaagg ctctggccgg tgaaacgcag
      241 cggcccgaaa gtaagcgcga tgtccgcgac cgctggcccg cgctgaccct ccgcgccgaa
      301 gaacaacagc cccaggccct gcatcgccag atggcaaccg accgaggcaa tcagcagcac
      361 aaggaccgaa gtatgcgcga gcggttgaaa ggcgaggcgg tagaggaaca gcccgatgca
      421 agccacgacc gcaagcgaaa gcgcgatcga gaccccgatc ggcggatggc ggctgacgac
      481 cgaaagcgtc gccgcgagca cggcgccggg cagcaagatg tcgactgcga aggcccgcgc
      541 gatccgtttg ccgtcgaggg cgcggcgcgc cgcgatgagg tcgaagacga atgcggccgc
      601 gcccatcgcc atcgcaagct ttgcggtgcc gggcatctgg cccgacgaca gcgaggcata
      661 ggtcagcgcg ccataggtga cgaactcgcc ctgcggaatc aggatcacgc gcgtcacggc
      721 gaataccaga accagcgcca gcccgagcag cgcatagatt gcgccgttgg tgatgccgtc
      781 ctggatcagg aacagcatga tcgtggagtt caagaccgga cgctccccct caagccggtt
      841 cgaccggtcg caacatcgaa ggcgccagcc gttggcagca gttgaaatgc tccgccgaca
      901 tttgatatgg tggataacaa ttatcgaata taatctcaag gccgagaagc gcggccgcgt
      961 ttcaacctac cgggactttg agccgcacgc gatgactgtt tccaagaccg cttttgccga
     1021 cggggcacgc acccgcaagg aggccgtcga cggggccggc gaaggcagcg cgctgcagct
     1081 cggcgagttg tcggaattgc tcggctattc gctcaagcgc gcgcaactca ggatcttcga
     1141 ggacttcctg cgctgtgtcg cgccgttgca actgacgccg gcgcagtttt ccgtgctgct
     1201 gctgctcgac aaaaaccccg gccgtaacca gaccgagatc gccaacacgc tcggtatcct
     1261 gcgaccgaat ttcgtggcga tgctcgacgg cctcgaaagt cgcgatctct gcacgcgcgt
     1321 gcgctccacc aacgaccgcc gctcccacgt tctgctgctg accgacaagg gcaaggccgt
     1381 gctggcgcgt gccaagaaac tcgtcgccac aaagcacgag gcgcgcctga atgagctgtt
     1441 gggtgcaccc aatcgtgccg cgctgctcgc gatgctgacc aagatcgcgc aggaattttg
     1501 attcctgctg acggcgcgag ccgaactgca atcactcgac caaaatcacc cggatatcgt
     1561 tgacattggt caacgtcggc ccggtcagca ggagatcgcc ggtggcggcg aagaacgcgg
     1621 tcgcgtcgtt attgtcgaga taggcgcgcg ggtcgagccg ttgcgaccgc atctttgcga
     1681 aggtcgccgc atccatcatc gcgccggcgg gatcggtcgc gcttcctgcg ccgccgtcgg
     1741 cgccgtcggt gtcgccggcg agcgccgcga tgccggtcgt atccttgagc agatcggcca
     1801 gcgccagcgc atattcctga ttgggcccgc cgcgcccgtt gccgcgcacg gtgaccgtca
     1861 gctcgccgcc ggagaggatc gcgacccgct tgccttgcgc gcgcgcggca agcgccagcc
     1921 gcgcatgatc ggccgcgact tcgcgcgctt cgccctcgag gtcggcgccg agatcgatcg
     1981 tctcgtagcc ggcgtccttc gcgagcctga tcgcggcatc gagcgaggcg cgcgggcgcg
     2041 cgatcagctc gagcctcgta cgggcgaatg cgggatcgcc cggcttgcag ctttcgttgg
     2101 cggaatcttc cagcgcgcgg cggatcgcgt catcgacctc gagtccgtat ttcgcaacga
     2161 tcgcgcgtgc atccgccagc gtggtcggat cgggcacggt cggacccgag gcgattgcgg
     2221 acggatcgtc atgcggcacg tcggaaatcg ccagcgtcac gatctcggcg cgttggccgg
     2281 cacgcgcgag ccggccgccc ttgatccgcg ataaatgttt tctcaccgtg ttgacctcgc
     2341 cgatcggtgc gccggagcgc aagagcgcgc gggtgagttt ttgcttttgc gcaaaagtga
     2401 cgccgtcgac cggcgcgatc cagttcgccg agcctccgcc ggagagcagc accagcagga
     2461 gatcgtcctc cccggccgtt tgcgctagcc gcaaggtctc ttcggcggcc ttcagccctg
     2521 cttcatccgg catcgggtga cccgcctcga caacccggat ccgccgtgtc ggtacgccgt
     2581 ggccgtgacg ggttgtggcg atgccggaca gccgcgcggg atcgagttcg agcgcgtcga
     2641 ggtaatgccg ctcggcggcc gaggccatcg cggctgcgcc tttgccggcg gcaaggcaga
     2701 tcaccttgcc tttgggaaca ggacgcagat gcctttccag cacgacgtcg ggatgcgcgg
     2761 cggcgacggc agcatcgtag atggcgcgca ggagggggcg tcggtcggtc atggtgttgc
     2821 ggtcgactgc gaggcaaaca tgcaactgac cttatccatc cgattgccgg aaaagaaaac
     2881 ccccgcgagc ctcgcccgcg ggggcaaatt gcgatgtggc gacgaaagcg atcagccgcc
     2941 gacttctccg gagacgacgt cgccgaacag ttcccagcgc tcgcccttga atcgctccaa
     3001 ctgaagctgg ctgatcggag cgaaatcgct cggcgatgtg ttgatcatga cgcccggcag
     3061 caggacttcg gttcggaagt ccttcagatt ggccgcctgc cgcatgatgt tctcgcgcgt
     3121 gaggttgtcg ccgcactgtt tcaatacctg aaccagggtc tgggcgacgc cgtagccgac
     3181 gacgaccgac gcgtcgagct tgttgccttc cggaaaatcc ttggcgagga aatccaggaa
     3241 ctgcttcatg ccggcgtcat tgtcccactg cgggtcggag acgtcc
//
LOCUS       DATLJT010000713         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1882198,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000713 DATLJT010000000
VERSION     DATLJT010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882198"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VM910_16310"
     CDS             <1..504
                     /locus_tag="VM910_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI64128.1"
                     /translation="PFIAGAAIGVPAGVALLTWSDPRSVRIAVGIILILYSVYAFFRP
                     ALKLSGGGRAGDAAVGLLNGVLGGLTGLAGILVTIWCGLRGWPKDVQRTVFQPVAVAI
                     FLMSAIWLGAKGTVTAETAKFFVMGLPCVLGGTWLGLKLFGRIDEATFRKIVLALLFL
                     SGVTLLF"
     gene            615..1433
                     /locus_tag="VM910_16315"
     CDS             615..1433
                     /locus_tag="VM910_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HVI64129.1"
                     /translation="MSSYNSISTEKLSRLIGTANAPILIDVRIDEDFAADPRLIPGSR
                     RYSHLDVQDWAGRLAGQSVVVICHKGQKLSEGTAAWLRHGNIAAEILEGGHVAWKEAE
                     LPTVPADKIPKRDGRGRTVWVTRERPKIDRIACPWLIRRFVDPAAVFLFVASPEVPAV
                     AERFNATPFDIENVFWSHRGELCTFDIMVREFGLSTPAMDRLATMVRAADTARLDLSP
                     EAPGLLAASLGLSRMFDDDLEQLNAGLLLYDAFYRWCRDATKETHNWPTNKAKP"
     gene            1450..1929
                     /locus_tag="VM910_16320"
     CDS             1450..1929
                     /locus_tag="VM910_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64130.1"
                     /translation="MKNSLRLLTLAVALQFAYPVYAASDSKTIVFVCLHGSVKSQMAA
                     AHFNRIARERGLPYTAVSRGIEVDASIPTKIRDGLSLDGLAPADDVPLPLTATEAEHA
                     VDVVAFDAVPDDRRGVAEVHYWSDVPPATRNYTAARDAIVRHIDDLVSALAARAAAP"
     gene            1971..>2433
                     /locus_tag="VM910_16325"
     CDS             1971..>2433
                     /locus_tag="VM910_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI64131.1"
                     /translation="MTVNTERRDFIKGVGVAAASTATLAGTQALAQANPQTGAKAMAY
                     QPKPMSFDPKSITGISEKVLVSHYENNYVGAVKRLNAIGAQLAELDFAKAPNFVVNGL
                     KREELVASNSMILHEIYFDGLGGSSNPRGPLIEAIVRDFGSVERWRAEFSAM"
BASE COUNT          446 a          777 c          764 g          446 t
ORIGIN      
        1 ccattcatcg ccggcgcagc catcggtgtg ccggcaggtg tcgcactgct gacatggtcc
       61 gatccgcgca gcgtccgcat cgccgtcggc atcatcctga tcctttatag tgtttacgcc
      121 ttcttccgcc ccgcgctgaa actttccggc ggcgggcggg ccggcgatgc ggcagtcggg
      181 ttactcaacg gcgtactcgg cggcctcacg ggcctggccg gcattctcgt caccatctgg
      241 tgcggactgc gcggctggcc gaaagacgtc cagcgcacgg tgtttcagcc ggtcgcggtt
      301 gcgatcttcc tgatgagcgc gatctggctt ggcgcgaagg gaaccgtcac tgcggagacg
      361 gctaaattct ttgtgatggg actgccctgc gtgctcggcg gaacctggct cggcctcaag
      421 ctgttcggcc ggattgacga ggcaaccttc cgcaagatcg tactcgcgct gttgttcctc
      481 tcgggcgtca cgctgttgtt ctgagaccgt tgacagcggc cacgacaggc ggtttattca
      541 agtgacgggg gcagcgatcc ccccgcgagg cgacatgccc aagaatcgcg tcctgtttca
      601 cgaaaggacg cagcatgtca tcctacaact cgatctccac cgaaaaactg tctcgcctga
      661 tcggcaccgc caatgcgccg attctcatcg acgtccgcat cgatgaggat ttcgcggccg
      721 acccgcgcct gataccgggc tcgaggcgct actcccatct cgatgttcag gactgggcgg
      781 ggcgcctcgc cggtcagtcg gtcgttgtca tctgtcacaa gggacaaaag ctcagcgaag
      841 gcacggccgc ctggcttcgc catggcaata tcgccgccga gattctcgaa ggcggtcatg
      901 tcgcctggaa ggaggccgag ctgccgaccg ttcccgccga caagattccg aagcgggacg
      961 ggcgcggccg caccgtctgg gtcacgcggg agcggccgaa gatcgatcgc atcgcctgcc
     1021 catggctgat ccgacgcttc gtcgatccgg ctgccgtctt cctcttcgtc gcatcgcccg
     1081 aagtcccggc ggtcgccgag cgcttcaacg ctactccatt cgacatcgag aacgtgttct
     1141 ggagccatcg cggcgaactc tgcaccttcg acatcatggt cagggaattc gggctctcga
     1201 cgccggccat ggatcggctc gcgacgatgg tgcgcgcggc agacaccgcg cgtctcgacc
     1261 tctcgcccga ggcgccgggc ctgctcgctg catcgcttgg gctatcgcgg atgttcgacg
     1321 acgacctcga acagttgaac gccggacttt tgctctacga cgccttctac cggtggtgtc
     1381 gcgacgcgac aaaggaaacg cacaactggc cgaccaacaa ggcgaagccg tgagaagggg
     1441 gcgacaacca tgaaaaattc actcaggctg cttacgctcg cggtcgcgct tcagttcgcc
     1501 tatccggtct atgcggcctc cgacagcaag accatcgtgt tcgtctgtct ccacggctcg
     1561 gtgaagagcc agatggccgc ggcgcatttc aacagaatcg cgagggagcg cgggctgcca
     1621 tatacggcag tctcgcgcgg catcgaggtc gatgcgtcga tccccacaaa aatcagggac
     1681 ggcttgagcc tggacgggct cgcgccggcc gacgacgttc cgctgccgtt gacggcaaca
     1741 gaagcggagc atgcggtcga tgtcgtcgct ttcgatgccg ttccggacga ccggcgaggc
     1801 gtggcggagg tgcattattg gtcggacgta ccgccggcga ccaggaacta caccgccgct
     1861 cgcgatgcga tcgtgcgcca catcgacgat ctcgtctccg cgctcgcggc acgagccgcc
     1921 gcgccataag cgcgggcacc ttcgggggca atcaaaaagt ggagagtctc atgactgtga
     1981 atacggagcg ccgggatttc atcaaaggcg ttggcgtcgc cgccgcatcg acggccacgc
     2041 ttgcgggaac gcaagcgctc gcgcaagcca atccgcaaac aggagccaag gcgatggcgt
     2101 atcagccgaa acccatgtcg ttcgatccaa aatccattac cggcatctcg gagaaggtgc
     2161 tcgtcagcca ctacgagaac aactatgtcg gcgcggtgaa acggctgaac gcgatcggcg
     2221 cgcaactggc cgagctcgac ttcgccaagg ctccgaactt cgtcgtcaac ggcttgaagc
     2281 gtgaggagct ggtcgcgtcg aactcgatga tcctccacga aatctatttc gatggactcg
     2341 gcggcagcag taacccaaga gggccgctga tcgaggcaat cgtgcgcgat ttcggcagcg
     2401 tggagcggtg gcgcgccgag ttctcggcga tgg
//
LOCUS       DATLJT010000714         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2801849,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000714 DATLJT010000000
VERSION     DATLJT010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801849"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1159)
                     /locus_tag="VM910_16330"
     CDS             complement(5..1159)
                     /locus_tag="VM910_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64132.1"
                     /translation="MLAALLLSLTHAARAEAAERPAASATAPSPAAMAEYRRKLEEYT
                     LARRKYESEADAYWGSIADKRRLRRSKRRNNQDVVLGDYVLTQPPVYSGPPKPVDPSA
                     PPEEVPPRKYVPVVADFLRAAAKEFNFVPQQPRSEMDYKRAYAKTASAAGLTKEQAVR
                     IYAFESGGDGKYDVQAGLEQPKPGAQAISTALGYNQLLATNSVELMAEKGDQFIRTLS
                     AKAAGLPEEAKKPLQRKLGVLKNMIAFCRSVPDTWSAHDKLANTPKGLGVHALNLDLD
                     VGPLLQTQKLLDSVVFARAQGYGAVLSAAELEMMNLTGDGNGLDIIMMPLAWRERVPT
                     SNFFQPGGYERNPIAGRSGVVSKLIASTNAVMDQESKLQGAKDLAAVFPK"
     gene            complement(1224..2231)
                     /locus_tag="VM910_16335"
     CDS             complement(1224..2231)
                     /locus_tag="VM910_16335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64133.1"
                     /translation="MPVIDIHTHCSPPARDDPLGVAQALRGIPVGKNSVTNYRGLPAV
                     SYHDMTDFDLQQEVCVKAGITGRIISNPFAAEVMTAISRKPAIDVVKHVNDRTAAVVA
                     RAKSNWGLGTLNPLDASHIAEGERCLGALGFKGLLITSSWHGQFIDAEDAFAFWEWAQ
                     ARQVPIFIHPPRVPIGHEQQMDQYKLDELVGRPFDTAMALARMILSGLFDRYPDVKIA
                     VAHMGGGLLPAIGRLDFGWRLGCEGMPERAVIRCKQRPSDYLAKLYVDTMGFWAPHVR
                     ETVEVFGADHVMFGTDYGPVPIDPGEHVDIVNGLVISAADKEKILWRNAVRFFNLDNL
                     A"
     gene            complement(2231..>2507)
                     /locus_tag="VM910_16340"
     CDS             complement(2231..>2507)
                     /locus_tag="VM910_16340"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968640.1"
                     /note="E3 component of alpha keto acid dehydrogenase
                     complexes LpdC; forms a homodimer; binds one molecule of
                     FAD monomer; catalyzes NAD+-dependent oxidation of
                     dihydrolipoyl cofactors that are covalently linked to the
                     E2 component; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVI64134.1"
                     /translation="VGRFPFIGNGKAIALGEDQGLVKVIFDKKTGALLGAHMVGAEVT
                     ELIQGYVVAMNLETTEEELIHTVFPHPTLSEMMKEAVLDAYGRVLNI"
BASE COUNT          455 a          799 c          749 g          504 t
ORIGIN      
        1 ggattcattt ggggaatacg gccgccaggt cctttgcgcc ttgcaatttg ctctcttgat
       61 ccatcactgc gttggtcgaa gctatcaact tcgagacgac gccgctgcgg ccggcgatcg
      121 gattgcgctc atagcccccc ggctggaaga agttcgaggt gggcactcgt tcgcgccatg
      181 ccagcggcat catgatgata tcgaggccat tgccatcgcc ggtgaggttc atcatctcga
      241 gctcggccgc actcaacacc gcgccatatc cctgtgcgcg cgcgaacacg accgagtcca
      301 agagcttctg cgtttgcagc aaaggaccaa cgtccagatc cagattcagc gcatggacgc
      361 cgagaccttt gggggtgttc gccagcttgt cgtgggcgct ccaggtatcg ggcacgcttc
      421 ggcagaaagc gatcatattt ttgagcacac caagtttccg ctgcagtggt ttcttcgcct
      481 cctcgggtaa tcctgcggcc ttcgcgctca aggtccggat gaactgatcg cccttctccg
      541 ccatgagctc gacgctgttg gtggccagca gctggttata acccagcgcg gtagaaatcg
      601 cctgggcgcc cggcttcggc tgttccaacc ctgcttgcac atcgtatttg ccgtcgccac
      661 cggactcgaa agcataaatt cgcacggcct gttccttcgt cagcccggcg gcagaagcag
      721 ttttggcgta ggcgcgtttg taatccatct cgctgcgagg ctgctgcgga acaaaattga
      781 attccttcgc cgctgcccgc aaaaagtcgg ccacgaccgg cacatacttt cttggtggca
      841 cttcctcggg aggcgccgag ggatcgaccg gtttgggcgg gcccgaatag accggcggct
      901 gggtcaatac gtaatcgccg agcacgacgt cctgattgtt gcggcgtttg gaacgtcgca
      961 gccgtcgctt gtccgcgatg gaaccccagt aagcatcggc ttcgctctca tatttccgac
     1021 gcgcgagggt gtactcctcc agtttgcgac gatattcggc catggcggcc ggcgacggtg
     1081 cggtcgccga cgccgccggg cgctcggcag cttcggctct cgctgcgtga gttaacgaga
     1141 gcagcaacgc cgcgagcacg cacccgcagc acctatggag cgaaccgtgc cgcatttcgt
     1201 ctgcagtcct tgccgagcgg acctcacgcg aggttgtcca ggttgaaaaa ccgcaccgcg
     1261 ttacgccaaa gaatcttctc tttatcggct gcggagatta ccagtccgtt cacgatgtcg
     1321 acgtgctctc cgggatcgat cggcaccggg ccgtaatcgg tgccgaacat gacgtgatcg
     1381 gcgccgaaca cctccaccgt ctcgcgcacg tgcggcgccc agaagcccat ggtgtcgacg
     1441 taaagcttgg cgagataatc gctcggccgc tgcttgcagc gaatgacggc gcgctcgggc
     1501 atgccctcgc agccgagccg ccaaccaaaa tcgagccgtc cgatagccgg cagaagcccg
     1561 ccacccatat gtgcaaccgc gatcttcacg tctggatagc ggtcgaacag ccccgacagg
     1621 atcatgcgtg cgagcgccat cgcggtatcg aacggccgcc cgaccaattc atcgagcttg
     1681 tattgatcca tctgctgctc gtgtcctatg ggcacgcgcg gcggatggat gaagatcggt
     1741 acttgccgcg cttgcgccca ttcccagaac gcgaaggcgt cttccgcgtc gatgaattgg
     1801 ccgtgccagc tcgacgtgat cagcagcccc ttgaagccga gcgcgccaag acagcgctcg
     1861 ccctcggcga tgtggcttgc atcgagtgga ttgagcgtgc cgaggcccca attgctcttc
     1921 gcccgcgcca ccaccgcggc ggttcggtca ttgacgtgct tgacgacgtc gatcgcgggc
     1981 ttgcgcgaga tcgcggtcat cacctccgcc gcgaacggat tgctgatgat acggccggtg
     2041 atgccagctt taacgcaaac ctcctgctgg aggtcgaaat ccgtcatgtc gtggtaggac
     2101 accgccggca agccgcgata attcgtcact gaatttttgc cgaccggtat tccccgcagc
     2161 gcttgcgcca cgccgagtgg atcgtcgcgc gccggggggg aacaatgggt gtgaatgtcg
     2221 atgacgggca ttaaatgttg agcacacgcc cataggcatc gagcaccgcc tccttcatca
     2281 tctcggagag cgtcgggtgc gggaagacgg tgtggatgag ctcctcctcc gtggtctcga
     2341 ggttcatcgc cacgacatag ccctgaatca gctcggtcac ctccgcgccg accatgtgcg
     2401 cgccgagcag cgccccggtc ttcttgtcga agatcacctt gacgaggccc tggtcctcgc
     2461 ccagcgcgat cgccttgccg ttgcctatga acggaaagcg gccgacg
//
LOCUS       DATLJT010000715         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3720249,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000715 DATLJT010000000
VERSION     DATLJT010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720249"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VM910_16345"
     CDS             complement(<1..1250)
                     /locus_tag="VM910_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HVI64135.1"
                     /translation="MSAVAMQSATRAHEAGSHAATDLEKLPVEKVLAQLVVKPEQGLS
                     SAEARQRLAKYGPNALVEKEVSLLRKIVGHFTGPNAYMIEAAALVSAIIGHWDDFAII
                     AALLFFNAGLEFWQDRKASNALAALRKGLAPEATVMRDGKWQTLAAATLVPGDIVKIR
                     LGVIVPADLRLVGGDYASIDQAALTGESLPVAKKIGDEAYSGSIVKQGEMQGVVIATG
                     ANTFFGRTAKLVAGAGAVSHAQKAMFEIGNFLIIVAVALALIMVAVQVYQDIVITDDW
                     GLEDALGILQFVLVLLVASIPVAMPAVFSITMALGALALSKQKAIVSKLSAIEEMAGV
                     DILCSDKTGTLTKNQLTLGDPILFSAKSAQDCILAAALASKIEDRDAIDTAVIAALKD
                     QSALKSYKQVKFVPFDPVSKRTAAT"
     gene            1506..1826
                     /locus_tag="VM910_16350"
     CDS             1506..1826
                     /locus_tag="VM910_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64136.1"
                     /translation="MRALIAVAVLGLSLTAAHAGPCSEKIAQFEQAVRQSAGNPNAGP
                     MARQTIGAQLDRQPTPASVKRAQERAHSTFAANLARAKRLDAQGNRAGCTRALAAAKR
                     MYNF"
     gene            2016..2543
                     /locus_tag="VM910_16355"
     CDS             2016..2543
                     /locus_tag="VM910_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64137.1"
                     /translation="MLRRLLPLAFVTLSLMAPMASVAAQPFTQGAAASAISAANALSA
                     RIRGYHRWQGLTPPPASYCATMREGEAALKQLSRLASRAILYRAPGLALSLQQAGDRL
                     SDELDEEEEVNSQSGISYTLYPCPVPAPYPPRAPVLRILDAKAPACRRRADALRVSFD
                     ARRALMQRCLRIPGT"
     gene            complement(2642..>2938)
                     /locus_tag="VM910_16360"
     CDS             complement(2642..>2938)
                     /locus_tag="VM910_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI64138.1"
                     /translation="DDILIAGFRANAKNPADYKLLDKSYPSDPYALMIRKDDRQFKTL
                     VDETLAKLMRSGEFEKLYTQWFESPIPPRGINIQLPMSDALKRTIKEPNDRPNS"
BASE COUNT          529 a          905 c          963 g          541 t
ORIGIN      
        1 gtcgccgcgg tgcgcttgct gaccggatcg aagggcacga atttcacctg cttgtagctc
       61 ttgagcgcgc tctgatcctt caatgccgcg atcaccgcgg tgtcgatggc gtcgcgatcc
      121 tcgatcttcg aagccaaggc cgcggccagg atgcaatcct gcgcgctttt ggcggaaaac
      181 aggatcgggt caccgagcgt gagctggttc ttggtcaagg tcccggtctt gtccgagcac
      241 aaaatgtcga ccccggccat ttcctcgatg gcggacagct tggagacgat cgccttctgt
      301 ttcgacagcg ccagcgcgcc caacgccatg gtgatcgaga acacggccgg catcgccacc
      361 ggaatcgatg cgacgagcag aacgaggacg aattgcagga tgccgagcgc gtcttcgagt
      421 ccccagtcgt cggtaatgac gatgtcctga tagacctgga ccgcgaccat gatcagcgcc
      481 agcgcgacgg cgacgatgat caggaaattg ccgatctcga acatcgcctt ttgggcgtgg
      541 cttacggcgc cggcgccggc cacgagcttg gcggtgcggc cgaagaacgt attggcgccg
      601 gtcgcgatga cgacgccttg catctcgccc tgcttgacga tgctgcccga ataggcctcg
      661 tcgccgatct tcttcgcaac cggcaaggat tcgccggtca gcgccgcctg gtcgatggag
      721 gcgtaatcgc caccaacgag ccgcaggtcg gcgggcacga tgacgccgag cctgatcttg
      781 acgatgtcgc cgggcaccag cgtcgcggcc gcaagcgttt gccacttacc gtcgcgcatg
      841 acggtggcct cgggggcaag ccccttcctg agcgcagcca gcgcattgga ggccttgcgg
      901 tcctgccaga attcgagccc ggcattgaag aacaacagcg ccgcgatgat ggcgaaatcg
      961 tcccaatgcc cgatgatcgc cgaaacgagc gcggcggcct cgatcatgta ggcgttcggc
     1021 ccggtgaagt gccccacgat cttgcggagc aggctgacct ctttttcaac gagcgcgttg
     1081 gggccgtact tggcgagccg ctgccgcgcc tcggcgctgc tcagtccctg ctcgggcttg
     1141 acgacgagct gggcaaggac cttctcgacc ggtagtttct cgaggtcggt cgcggcgtgc
     1201 gacccagcct cgtgcgcgcg cgtggcggat tgcatggcaa cggcggacat ggactgctcc
     1261 tgggaacgca ttcgcgggat taagaaaacg gatttccaaa gctaacgaaa tgcggcttaa
     1321 tgatcgagat caaagaccag cccacaccgg gggagtcaac gtgctcggcg aggatgggtg
     1381 tcgactgatg gacgacctgc ggtggatcct ggtgaccagg gggatgaccc agcgtctcac
     1441 atgtcgatcg ggtgggatca gctcctaggt ggcgtatcat ggtgcccagt cacgagagaa
     1501 tagcaatgag agcattgatc gccgtcgccg ttctgggcct gagccttaca gcggcgcatg
     1561 ccggcccctg cagcgagaag atcgcgcaat tcgagcaggc ggtgcgccag tccgccggaa
     1621 atccgaacgc cgggccgatg gcgcggcaaa cgatcggcgc gcagctcgac cgccaaccga
     1681 cgcccgcctc ggtcaaacgg gcgcaggagc gcgcgcactc gacgttcgca gcgaacttgg
     1741 cgcgcgccaa gcggctcgac gcccagggaa atcgcgcagg ctgcacgcgg gcgctcgccg
     1801 ccgccaagcg aatgtataat ttttagcgct cggtcgggct cacgagcggc gctctcgggc
     1861 gaaacaaaga ggaacggcgg gatggtgatc ggcacgagag gcgacgattg gatgcccatt
     1921 cttgcgctcg cacgctggcg cgccagggag attgttgtcg atggccatgc ttcacgctag
     1981 ttgttgaaca tccgagcgcg ggctggggca cgcttatgtt gcgccgttta ttgccattgg
     2041 cttttgtcac gctcagcttg atggcgccga tggcgagcgt cgcggcgcag ccgttcacgc
     2101 aaggggcagc cgctagtgcg atctcggcgg ccaacgcgct cagcgctcgc attcgcggct
     2161 accaccgctg gcaaggcttg acgccgcccc cggcgagcta ttgcgccacc atgcgcgagg
     2221 gcgaggcggc gttgaagcag ctttctcgtc tcgccagccg agccatcctc taccgcgcgc
     2281 ccggcttggc gttgagcttg caacaggcgg gcgaccggct cagcgacgag ctcgacgagg
     2341 aggaggaggt caattctcag tccggtattt cctacacgct ctacccctgt ccggtaccgg
     2401 cgccgtatcc gccgcgcgca cccgtgctca ggatcctcga cgcaaaggcg ccggcctgtc
     2461 gcaggcgggc ggatgcgctt cgcgtgtcgt tcgacgcgcg ccgtgcgctg atgcagaggt
     2521 gcctgcgaat accgggaacg tagccgttat cgcttgcacg agcatcgtca cagtcgttat
     2581 cgcgcggcgc ttgggtgcca cggataggac agataaatag agcgcggtgc ggcaacgacg
     2641 ctcacgaatt tggcctgtcg ttcggctcct ttatggtgcg cttgagagcg tccgacatcg
     2701 gcagctggat gttgatgccg cgcggcggga ttggactctc gaaccactgc gtatagagct
     2761 tctcgaactc gcctgagcgc atcagcttgg ccagcgtctc gtcaaccagc gtcttgaatt
     2821 gccgatcgtc cttccgaatc atcagcgcgt aagggtcgga cggataagat ttgtcgagca
     2881 gcttgtagtc ggcgggattt tttgcattcg cccggaagcc cgcgattaaa atgtcgtc
//
LOCUS       DATLJT010000716         4766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3720251,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000716 DATLJT010000000
VERSION     DATLJT010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720251"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VM910_16365"
     CDS             complement(<1..1167)
                     /locus_tag="VM910_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64139.1"
                     /translation="MRARISMLLATLAIALPAFTAQAETVVRYGISMADIPLTTGQPD
                     RGAGAYQFTAYTIYDPLVAWEMDVSDRPGKLVPGLATDWKVDDADKTKWRFILRKGVK
                     FHDGSDFNADAVIWNLDKVLNDKAPQFDKRQSAQVKTRLPSVASYAKIDDSTVEITTK
                     TVDSFFPYQMLWFLVSSPAQYEKLGKDWDKFASQPSGTGPFKLTKLVPRELAELSKNE
                     DYWNKNRIPKVDKIVLIPMPEALTRTNALLAGQVDLIETPAPDAVPQLKAAGMRIVDN
                     ITPHVWNYHLSVLPGSPWTDIRLRKALNLAIDRDAVVGLMNGLAKPAKGQVDPSSPWF
                     GKPTFDLNYDVAAARKLVEQAGYSKEKPLKTTFIIAQGGTGQMLSLPMNEFLQQS"
     gene            1404..3029
                     /locus_tag="VM910_16370"
     CDS             1404..3029
                     /locus_tag="VM910_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64140.1"
                     /translation="MMLVVVGLATALPQAAAAETVLRIGMTAADIPRTLGQPDQGFEG
                     NRFTGLTMYDALTMWDLSSADKASVLLPGLATEWKVDDADHKKWVFKLRPGVKFHDGS
                     DFNADAVVWNVEKVLKKDAPQFDASQVGVTASRMPTLVSAKKIDDLTVELTTSEPDSF
                     LPINLTNLFMVSPAKWQKFFAAASGADAKAKAQAAWDAFARDASGTGPWKMSSFTPRE
                     RLEVAKNENYWDKARVPHVDKMVLLPMPEANARTAALLAGQIDWAEAPAPDALPEIKQ
                     RGFVIYSNEEPHVWPWQFSRVEGSPWNDVRVRKAANLCVDREGLKDGLLGGLMVPATG
                     TFEPGHPWRGNPTFQIKYDKAAAQKLMQEAGYGPSKHLAVKIQTSASGSGQMLPLPMN
                     EYLQQALAECYFDVQLDVIEWNTLFTNWRRGAKDPSANGANATNVTYAAMDPFFALVR
                     FLQSSMAPPVSNNWGYINNPKFDELVTKARTTFDPAERDKVLADLHAASVDDAAFLYV
                     AHDVAPRAMSPKIKGFVQPKSWFVDFSPITMTQ"
     gene            3075..4028
                     /locus_tag="VM910_16375"
     CDS             3075..4028
                     /locus_tag="VM910_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64141.1"
                     /translation="MLVYVARRIVYVIPIVVSVALVCFMLVHITPGDPLVAVLPADAS
                     QELAAQLRAAYGFDRPLPVQFGLWLWRAVNGDLGHSIATGRPVLTEVMRAVGNTVTLA
                     IAAAMIGFTLGLVFGLIAGYFRDGWIDKLATSIAIAGVSVPHYWLGMVLVIIFSVELN
                     WLPAVGAGPGGSGAWGWDWEHFRYLILPAITTSVIPMGIVTRTVRALTGDILSQDFVE
                     ALRAKGLRETGVFRHVVKNAAPTALAVMGLQLGYMLGGSILIETVFSWPGSGLLLNSA
                     IFQRDLPLLQGTILVLALFFVILNLLVDIAQAAIDPRIKRG"
     gene            4057..>4766
                     /locus_tag="VM910_16380"
     CDS             4057..>4766
                     /locus_tag="VM910_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64142.1"
                     /translation="MSDAALQAAPQTKARGYWATVGRRILHDKVSMACALILIAIFLS
                     ALIAPWLGLADPYQGSMIRRLRHIGTPGFPLGTDELGRDMLARLVYGGRLSLIVGISP
                     VILAFCIGSSLGLVAGYVGGKVNTAIMRTVDVFYAFPSVLLAIAISGALGAGIVNSIV
                     SLTIVFVPQITRVAESVTTGVRNRDFVEAARASGAGPFTIMRVHILGNVLGPIFVYAT
                     GLISVSMILAAGLSFLGLG"
BASE COUNT          818 a         1484 c         1502 g          962 t
ORIGIN      
        1 actctgttgc aggaattcgt tcatcggtaa cgacagcatc tgcccggtgc cgccttgcgc
       61 gatgatgaag gtggtcttca gcggtttctc cttggagtag ccggcttgct ccaccagctt
      121 cctggctgcc gcgacatcat aattgaggtc gaaggtcggc ttgccgaacc acgggctcga
      181 cgggtcgacc tggcccttgg cgggcttggc caatccgttc atcagcccga ccaccgcatc
      241 gcggtcgatc gcgagattga gcgccttgcg taaacggata tcggtccagg gcgagcccgg
      301 cagcacgctc aaatgataat tccagacatg cggagtgatg ttgtcgacga tgcgcatgcc
      361 ggccgccttg agctgcggca ccgcgtcggg cgcaggcgtc tcgatcaggt cgacctggcc
      421 ggcgagcagc gcattggtcc gggtcagcgc ctccggcatc gggatcagga caatcttgtc
      481 gaccttcgga atccggttct tgttccagta atcctcgttc ttgctgagct cggcgagctc
      541 gcgcggcacg agctttgtca gcttgaacgg cccggtgccg gacggctggc tggcaaactt
      601 gtcccagtcc ttgccgagct tttcatattg ggcggggctg gagaccagaa accacagcat
      661 ctgatagggg aagaaggaat cgaccgtctt ggtcgtgatc tccacggtgg agtcgtcgat
      721 cttggcatag cttgccactg agggcaggcg ggtcttgacc tgtgcgctct gccgcttgtc
      781 gaattgcggc gccttgtcat tcagcacctt gtcgagattc cagatcaccg cgtccgcgtt
      841 gaaatcgctt ccgtcatgga acttgacgcc cttgcgcagg atgaagcgcc atttggtctt
      901 gtcggcgtcg tccaccttcc agtcggtggc gagccccggc accagcttgc cgggcctgtc
      961 cgacacgtcc atctcccagg cgaccagcgg atcgtagatg gtataggccg tgaactgata
     1021 ggcgcccgcg ccgcgatccg gctgacccgt ggtcagcgga atgtccgcca tcgaaatgcc
     1081 gtagcgcaca acggtttcgg cctgcgctgt gaaagcgggc agggcgattg ccagcgtggc
     1141 aagaagcatc gaaattcgcg cgcgcatggc ctaatctcca ttgaaataat cggggtcaag
     1201 gcctgcaagg ttgatgccag attaggcagt gctgttcgtt cttcatgcgg cgggggaaga
     1261 atctgcttcg cccgcggtta tgcggcaaag aactatcctc cttggcacag ccgttgcata
     1321 agtttgcata agaattagtc acaccaagtt gaacaggggt tgttgcgatg cgtaacgaga
     1381 aaaccgggaa aacagccact gcgatgatgc tggtggtggt gggcctggcg acggccttgc
     1441 cgcaggcagc ggcggccgaa accgtgctgc gcatcggcat gaccgcagcc gatattcccc
     1501 gcacactcgg ccagccggac cagggttttg agggcaaccg ctttaccggc ctcaccatgt
     1561 atgacgcgct cacgatgtgg gacctctcct ccgcggacaa ggcgagcgtg ttgcttccgg
     1621 gcctcgcgac cgagtggaaa gtcgacgacg ccgaccacaa gaagtgggtg ttcaagctgc
     1681 gaccgggcgt gaaattccat gacggcagcg acttcaatgc cgatgcggtg gtgtggaacg
     1741 tcgagaaagt cttgaaaaaa gacgcgccgc agttcgacgc cagccaggtc ggcgtcaccg
     1801 cctcgcgcat gccgacactg gtctcggcga aaaagatcga cgacctcacg gtggaattga
     1861 ccacctccga gcccgacagc ttcctgccga tcaatctcac caatttgttc atggtgagcc
     1921 cggcgaaatg gcagaagttt tttgccgcgg cgtccggcgc tgacgcaaaa gcgaaagcgc
     1981 aagccgcttg ggacgctttt gccagggacg cgtcgggcac cggtccctgg aagatgtcga
     2041 gcttcacccc gcgcgagcgg ctcgaagtgg cgaagaacga aaactattgg gacaaggcgc
     2101 gcgtgcccca tgtcgacaag atggtgctgt tgccgatgcc ggaagcaaac gcacgcaccg
     2161 cagcgctgct cgccggtcag atcgattggg ccgaggcgcc cgcgcccgac gcgctgcccg
     2221 agatcaagca gcgcggcttc gtgatctact cgaacgagga gccgcatgtc tggccgtggc
     2281 aattctcgcg cgtcgagggt tcgccctgga atgacgtccg cgtgcgcaag gccgccaatc
     2341 tctgcgtcga ccgcgaaggc ctcaaggatg gcctgcttgg cggattgatg gtgccggcga
     2401 ccggcacgtt cgagcccggc catccctggc gcggcaaccc caccttccag atcaaatatg
     2461 acaaggccgc cgcgcaaaag ctgatgcagg aggcgggcta cggtccgagc aagcatctgg
     2521 cagtcaagat ccagacctcg gcatccggct cgggccagat gctgccgctg ccgatgaacg
     2581 aatatctgca gcaggcgctg gccgaatgct atttcgacgt gcagctcgac gtgatcgagt
     2641 ggaacacgct gttcaccaac tggcggcgcg gcgccaagga tccttccgcc aatggcgcca
     2701 acgccaccaa cgtgacctat gcggcgatgg atccgttctt cgcgctggtg cgcttcctgc
     2761 agtcgtcgat ggcgcctccg gtgtcgaaca attggggcta catcaacaat cccaaattcg
     2821 atgagttggt gaccaaggcg cgcaccacct tcgatccggc cgagcgtgac aaggtgctgg
     2881 cggacctgca tgccgcctcc gtcgatgacg cggcatttct ctatgtcgcc cacgatgtcg
     2941 cgccgcgtgc aatgagcccg aagatcaagg gctttgtgca gccgaagagc tggttcgtcg
     3001 acttctcgcc gatcacgatg acgcagtgag ttggcccctc tcccgcaagg ggagaaggaa
     3061 gatcaaggtg atccgtgctc gtctatgtcg ccagacgcat tgtctacgtg atcccgatcg
     3121 tggtcagcgt cgcgctggtg tgcttcatgc tcgtgcacat cacgcccggc gatccgctgg
     3181 ttgccgtgct gccggccgat gcttcgcagg aactcgcggc gcagcttcgc gcggcctacg
     3241 ggttcgaccg gccgttgccg gtgcagtttg ggctgtggct ctggcgcgcg gtgaatggcg
     3301 atctcggcca ttccatcgcg accggacgac cggtcttgac ggaagtgatg cgtgcggtcg
     3361 gcaataccgt caccctggcg atcgcagccg cgatgattgg cttcaccctt gggctcgtgt
     3421 tcggcctgat cgccggctac ttccgcgacg gctggatcga caagctcgcg acctccattg
     3481 ccatcgccgg cgtctccgtg ccgcattact ggctcggcat ggtgctggtc atcatcttct
     3541 cggtggaact gaactggttg ccggcagtcg gcgccggccc cggcggctcc ggcgcctggg
     3601 gatgggattg ggagcatttt cgctatctga tcctgccggc gatcacgacc tcggtgatcc
     3661 cgatgggcat cgtaacccgc acggtgcgcg ccctgacagg cgacatcctc agtcaggatt
     3721 tcgtcgaagc gttgcgcgcc aagggtttgc gcgagaccgg tgtgttccgc cacgtcgtca
     3781 agaacgcggc tcccactgcg cttgcagtga tgggcttgca gctcggttac atgctcggcg
     3841 gctcgatcct gatcgagacc gtgttttcgt ggccgggctc gggcctgttg ctcaattccg
     3901 cgatcttcca gcgcgacttg ccgctcctgc agggcacgat cctggtgctc gcgctattct
     3961 tcgtgatcct caatcttctt gtcgacatcg cgcaggccgc gatcgaccca cgcatcaagc
     4021 ggggctagcg ccgtgaccat cacgccaatg gacgtaatgt cggatgcggc gctgcaggcg
     4081 gcgccgcaga ccaaagcgcg cggctattgg gcgaccgtcg gccgccgcat tttgcatgac
     4141 aaggtcagca tggcctgcgc gctgatcttg atcgcgatct ttctttccgc gctgatcgct
     4201 ccctggctcg gcctcgccga tccctaccag ggctcgatga tccgcaggct gcgccatatc
     4261 ggtacgcccg gctttccgct cggcaccgac gagctcggcc gcgacatgct ggcccggctg
     4321 gtctatggcg ggcggctgtc gctgatcgtc ggcatttcgc ctgtcatttt ggcgttctgc
     4381 atcggcagct cgctcgggct cgttgccggc tatgtcggcg gcaaggtcaa caccgcgatt
     4441 atgcgcaccg tcgacgtgtt ttatgctttt ccgtcggtgc tgcttgcgat cgcgatatcc
     4501 ggggcgctcg gcgccggcat cgtcaactca atcgtgtcgc tcaccatcgt gttcgtaccg
     4561 cagatcaccc gcgtcgccga aagcgtcacg acgggcgtgc gcaacaggga tttcgtcgaa
     4621 gcggcgcgcg cctccggcgc cggccccttc accatcatgc gcgtgcatat cctgggcaac
     4681 gtgctggggc cgatcttcgt ttacgccacc ggcctgattt cggtctcgat gatcctcgcg
     4741 gcgggcctgt cgttcctggg gctggg
//
LOCUS       DATLJT010000717         3961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1422572,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000717 DATLJT010000000
VERSION     DATLJT010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422572"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VM910_16385"
     CDS             complement(<1..1409)
                     /locus_tag="VM910_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HVI64143.1"
                     /translation="MKPIDLRIAEELGVRAQQVAAAIALLDSGATVPFVARYRKEATG
                     ALDDTQLRRLEERLRYLRELDERRAAILDSIGQQGKLDPALKQAIDAADSKARLEDLY
                     LPYRPKRRTKAQIAREAGLEPLAEALLASPDQNPEQVARRYVEPNKGVIDSAAALEGA
                     RAILVERFREDAMLIGTLREEVWSRGRLCSRVRAGKAEPGAKFADYFEFSEPLASLPS
                     HRILALFRGEKEEVLSLELNPDPVAPDRDELSAFERRIANRFKIIDRGRPGDRWLIDT
                     VRLAWRTRILAHIESDLRLRLWQAAEDAAVQVFAGNLRDLLLAAPAGACPTMGLDPGY
                     RTGVKVAVVTATGQVAATTTIYPHEPQRRWDESIAQLAQLIQQHRVELIAIGNGTASR
                     ETDRLGAELIRLHPELKLTKVVVSEAGASVYSASAFASQELPGLDVSLRGAVSIARRL
                     QDPLAELVKIDPRSIGVGQ"
     gene            2510..3652
                     /locus_tag="VM910_16390"
     CDS             2510..3652
                     /locus_tag="VM910_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64144.1"
                     /translation="MSAKFCLFCGERPEGKSKEHVLPRWLIELTGEPTRIANFGLDLS
                     QGVEAAAPRQYALSQFQFPACTVCNGIFSTLENEGKKVISKILASERLFARDISTLLD
                     WLDKVRVGLWLGFHQLDKNIEGIRPRFFVQHRIGRKDRLLVLASRTDERQLLSFIGPT
                     TVAFRFLPSALGLVINRLCFVNVSCDYLLARRLGFPYPPNPIISIARQTPDYCQLGRG
                     LHRVLRPIYQGSLQLDGAVFYQPMFPSELTWRPSTFYEAEYVKGHSLDWEAGVGGIYY
                     SKGDQFRWLDDKGQLTDITVDPRCEGAEKKMVRSVYSALKHLCTRLAPNYVYRTAEDR
                     KFIELRQKLVLKELHSLERGARSMPGRPWVKDPPPAPHDSGSPNCE"
BASE COUNT          782 a         1194 c         1175 g          810 t
ORIGIN      
        1 tattggccca cgccgatcga tcgcggatca attttgacca gctcggcaag cgggtcctga
       61 agccgccggg cgatcgagac cgctccgcgc aacgacacat ctagccccgg caactcctgc
      121 gaggcaaaag ccgaagcgga atagaccgag gccccggcct cggagacgac caccttggtc
      181 agtttgagct cggggtgtag tcgaatgagc tccgcaccga gccggtcggt ttcgcgcgag
      241 gcggtgccgt tgccgatcgc gatcagttcc acccgatgct gctggataag ctgcgccagc
      301 tgagcaatcg actcgtccca acgacgctgt ggttcatgcg ggtagattgt cgtcgtcgcc
      361 gcaacctgtc cggtcgcggt gaccacggcg actttgacac cggtccgata ccccggatca
      421 agccccatcg tcggacatgc gcccgcgggg gctgcgagca gcaggtcgcg caggtttccg
      481 gcgaacacct gcactgcggc atcttccgcc gcttgccaaa gtcggaggcg caggtcgctc
      541 tcgatgtgag ccaggattct tgtccgccat gccaagcgca ccgtatcgat cagccagcgg
      601 tcgccgggcc gaccacgatc gatgatcttg aaccggttcg cgatccgccg ctcaaaggcg
      661 cttagctcat cgcgatccgg tgcaaccggg tcagggttta actccaggct cagcacctcc
      721 tccttttcgc cgcggaacag cgctaggatg cggtgcgacg gcaagctagc caaaggttcg
      781 ctgaactcga agtagtcggc gaacttggcg ccgggctccg ccttacccgc acgaactcgc
      841 gaacagagac ggccgcgcga ccacacttcc tcccgcaagg tcccgatcag catggcgtct
      901 tcgcggaaac gctcgacgag gattgcgcgc gcgccttcga gagcagcggc cgaatcaatc
      961 acccccttgt tcggttcgac ataccggcga gccacttgct ccgggttttg atcggggctt
     1021 gcgagaagcg cctctgccag cggctcgagg cccgcttcgc gcgcgatttg cgctttcgtc
     1081 cgccgcttcg gacgataggg cagatagaga tcctcgagcc gcgccttgct gtccgccgca
     1141 tcaatcgcct gcttgagtgc ggggtcaagt tttccttgct gaccgatgct gtcgaggatg
     1201 gcggcccgac gctcgtccag ctcgcgcaga tatctcagcc gctcctcgag cctacgtagc
     1261 tgggtgtcat cgagcgcccc ggtcgcctcc ttccggtagc gggcaacaaa gggcacggtc
     1321 gccccgctat caagcaacgc gatggcagca gcgacctgct gcgcgcggac gcccagttcc
     1381 tcggcgatgc gtaggtcaat cggcttcatc aattttgagc tattttcgag atggcggtga
     1441 aaccgcccaa gcagcctttc tatcgtgccc taggagaacc ggtcagcatg ccgttcagat
     1501 cctgttgaca aaatgaccag gcgggtacgc gagatcatgc gcgatggcgt aattgtggtg
     1561 tcgacccgat cgcggtgtct cgcatatcaa caacctcggg acgctatcgc cgggcaccgg
     1621 tcgagttaaa gcccgaaagc gagccggctg acggggcccg gtattggctt ccttcccgag
     1681 gcccggtgcc ccggcgttga tatcgttgcc ggagcgagcc cggcacggca catggtaaac
     1741 ctgccctcgt cgatcgtcaa aatccggttc tggccaccgg gccggtctgt cccagccgac
     1801 ctccgaaaaa tggggtcctc cgccgggacc gacggttcga atccacttcc ctccagcgga
     1861 gagtcaggcg aaatccatga gcgagcgtgt ggcgcggagg tttagactca tctggtgcat
     1921 cgtaaaccgg ccatgaagct cgttcccgcc caggcttcgt cagcatagac catggtcccc
     1981 ctggcgatcc gggtgcggat gaaattaagc gcagcgacgt ctgaggggta aaacgcgccg
     2041 ctcccttacc tttacggcat ctcatttatt gctgacgggc caggggtgat tcgacaatgg
     2101 acgaaaatcg gggcgtgggc ccgactgaga aacctgcctg acgccgcacc ccatcaggct
     2161 tgacggcgac ggtttacgtc atcgtgggcg cttcggcggc gagttttcca aattcctcgg
     2221 gagtgaacgc ccggacggtc ccgttgaccg aatgagacat cgcaagcgtt ggaaattgct
     2281 cgtgagcgcc gcggcaacgt gtccaactcg atctattcga cggccaccat tcgattgcgt
     2341 cagcggcgct ggctgagaat aaagaatttc gcatcgagca gatcgctctc agcggacggc
     2401 cagtcggtgc gggagatcgc ccggttttct gacttacgtc gtaatcggcc gtaaacgaat
     2461 gcggcaacac cattgagttg gcctccatga gtaatttagc gcgctaccta tgtccgcaaa
     2521 gttctgcctt ttctgcggtg agcggcccga ggggaagagc aaggagcacg ttctcccgcg
     2581 atggctgatt gagctcacgg gcgaaccaac gcgcatagcg aacttcggtc tcgacctctc
     2641 gcagggcgtc gaggccgcgg cgccgcggca gtacgcgctt tctcagtttc agtttcccgc
     2701 gtgtaccgtc tgcaatggga tcttcagcac actggaaaac gagggaaaaa aagtcatctc
     2761 taaaattctc gcttcagaaa ggttgtttgc gcgggacata tccaccttac tggactggct
     2821 cgacaaggtg cgcgtaggtc tttggctcgg cttccatcag ctcgacaaaa atattgaggg
     2881 gattcgcccg cgtttttttg tgcagcatcg aattgggcga aaggatcggc tgctcgtttt
     2941 ggcgtctcga accgacgaac gacagctctt gtcatttatc gggcccacta ccgtggcgtt
     3001 tcgcttcctt ccctccgcgc ttgggctcgt tataaaccgc ctgtgtttcg taaatgtttc
     3061 gtgtgattat ctcttagcaa gacgcctggg atttccttat ccgcccaacc cgatcatttc
     3121 catcgcgcgg caaacgcccg actattgtca gctcgggagg gggcttcatc gcgtattgcg
     3181 gcccatttat caagggtcat tgcaactcga cggggcagtc ttctatcagc cgatgtttcc
     3241 atccgagctg acatggcggc ccagcacttt ctacgaagcc gagtatgtaa aggggcatag
     3301 cctcgattgg gaagcggggg tcggcgggat ttattactca aagggagatc agtttcgctg
     3361 gctagacgat aagggtcagc tcaccgatat caccgttgat ccccgctgcg agggtgcaga
     3421 aaagaaaatg gttcgctccg tttattccgc ccttaaacat ctatgtactc ggctcgcccc
     3481 gaattacgtc tatcggaccg ccgaagaccg taaatttatc gagttgcgcc agaaacttgt
     3541 ccttaaagag cttcatagtt tagagcgcgg ggcccgttcg atgccgggcc gaccttgggt
     3601 gaaggatccg ccacctgcac cgcacgactc cggttccccg aactgtgaat aacggcgacg
     3661 tagctactag ttggatgatt caagacttaa gcaacagcct tcgatcaccc gaggtggact
     3721 ggatagaacg cttatccgac agtgccgtac tacgaggaca tcaacttagt agcaccaatt
     3781 atctgcggcg taggttggtg aagaaatccg gtgcgaacgt ctgcaatggg tcgaagatcg
     3841 ggataggggg acacctcgcg gcgccacccc tcccaaacca ccgggcatac gggtccgtac
     3901 cacggcggtt cggtcgggtt aagcgtgggc aggaacgtag aggcggggaa gaccgagacc
     3961 g
//
LOCUS       DATLJT010000718         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1422620,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000718 DATLJT010000000
VERSION     DATLJT010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422620"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VM910_16395"
     CDS             complement(<1..459)
                     /locus_tag="VM910_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HVI64145.1"
                     /translation="MDGAGRLARSVRSLRPQADLKAVAIGAAVLMRGLLGFGRARPTP
                     VVDREPRSRLVAMIERHVPRRAGIAATVLLLFASAGLGVVRGGHVDEVSAAMSDTRNA
                     LANAIGFRITAVAINGRKQLTQDEVLAIGGVSGRSSLLFLDAAAVRERLKA"
     gene            complement(793..1785)
                     /locus_tag="VM910_16400"
     CDS             complement(793..1785)
                     /locus_tag="VM910_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HVI64146.1"
                     /translation="MRVTILFGGTNKERLVSVASAQALCEALPEADLWFWEADDSVHE
                     TQSKTLLGHARPFEHAFKPGGRRIASTIDQALDQAKSDGRVLVLGLHGGRAENGELQE
                     MCEMRGIPFTGSGSASSNLAFDKVAAKRFVEIAGVVAPKGVALEEIETALAEYGKLIA
                     KPTRDGSSYGLIFVNARQDLVAVRNAAATEDYLIEPLIPGVEATCGVLERSDGSVISL
                     PPVEIVPGEGTFDYTAKYLLKSTQEICPGRFSPEISAAIMDQALKAHCALSCSGYSRT
                     DFIVSDKGPIYLETNTLPGLTKASLYPKALKAQGIDFADFLRDQIVQAERRARK"
     gene            complement(1785..2711)
                     /gene="murB"
                     /locus_tag="VM910_16405"
     CDS             complement(1785..2711)
                     /gene="murB"
                     /locus_tag="VM910_16405"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVI64147.1"
                     /translation="MAPFTDITPELKAAMPELRGRLLANESLAPLTWFRVGGPAQVLF
                     TPSDEDDLAYFLKNLPQELPVYVVGVGSNLIVRDGGMPGIVVRLSPRGFGETAVQGDI
                     VVAGAAALDRRVAETAAGANLAGLEFYFGIPGTIGGALRMNAGANGAETKDVLVEATG
                     IDRNGTVHTFSNADMKFVYRNSGVDPSIVFTSARFRGVAAAPDAIRARMEEVQRHRET
                     AQPIREKTGGSTFKNPPGNSAWKLIDAAGCRGLRVGGAQVSQMHCNFLINTGNATGHD
                     IETLGETVRNRVKETSGIELHWEIKRIGIERT"
     gene            complement(2713..4116)
                     /gene="murC"
                     /locus_tag="VM910_16410"
     CDS             complement(2713..4116)
                     /gene="murC"
                     /locus_tag="VM910_16410"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HVI64148.1"
                     /translation="MRLPREIGPIHFVGIGGIGMSGIAEVLNNLGYTVQGSDAVDSAN
                     VARLRERGVKVTVGHKAENVDGADVVVVSTAIKRDNPELTAARAQRIPVVRRAEMLAE
                     LMRLKSCVAIAGTHGKTTTTTMVATLLDAGGLDPTVINGGIINAYGSNARLGAGDWMV
                     VEADESDGTFLKLPSDVVIVTNIDPEHLDHFGTFEAIQDAFRAFVENVPFYGFAVMCI
                     DHPVVQTLVGKIEDRRIITYGENPQADARLVDLVPMGAGSKFNVVIRDRKTDAAHEIT
                     GITLPMPGQHNALNATAAIAVAHQLGLSDDAIRKAIAGFGGVKRRFTRTGEWNGVTVI
                     DDYGHHPVEIAAVLKAARGSTNGKIIAVVQPHRYTRLQSLFEEFCTCFNDADAVVVAE
                     VYPAGETPIDGIDRDHFVLGLRAHGHREVVPLPKPADLAGIVKGLATRGDLVVCLGAG
                     SITQWAYALPGELKALG"
     gene            complement(4213..5313)
                     /gene="murG"
                     /locus_tag="VM910_16415"
     CDS             complement(4213..5313)
                     /gene="murG"
                     /locus_tag="VM910_16415"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HVI64149.1"
                     /translation="MDPKPMILLAAGGTGGHLFPAEALGVELIKRGFDVRLVTDSRAL
                     RYSGLFSKDMIDVVASETARGRNPLQLARAAITLAVGTFTAFRLMRRLKPKAVVGFGG
                     YPTLPPLIAARLCGIPGIIHEANAVLGRANRFLARRASAIATSLPGVFDRDKALAAKA
                     TAVGTPLRPPILAAASTNYAPPDATAPFHLLVVGGSQGARVMSDIVPGAIERLEPALW
                     SRLVLTQQVRDEDMARVRNVYDRLKIKCELAPFFTDLPARLASNHLIVSRSGAGTVAE
                     LAAIGRPSILVPLPGSIDQDQFANAGVLAKVGGGLRIAQTEFTPARLAAEISALAAEP
                     AKLAAMAASARTAGRLDAAERLADLVVKIAGI"
BASE COUNT          924 a         1736 c         1728 g          962 t
ORIGIN      
        1 ggccttcagc cgctcgcgca cagccgccgc atcgagaaac agcagcgagg aacgcccgct
       61 gacgccgccg atcgccagca cctcgtcctg cgtcaactgc ttgcggccgt tgatggcgac
      121 agccgtgatg cgaaatccga tagcgttggc gagcgcgttg cgggtgtcgc tcatggccgc
      181 gctcacctca tcgacatggc cgcccctgac gacgccaagg cccgcactgg caaaaagcag
      241 cagcacggtt gcggcaatgc cggcacgacg cggcacgtga cgctcgatca tcgccacaag
      301 gcgcgaccgc ggctcgcggt cgaccacagg cgtggggcgt gcgcggccaa atccgagaag
      361 cccgcgcatc agcacggccg caccaatggc aaccgccttc aggtcagcct ggggcctcag
      421 cgatctaacc gaccgggcga ggcgtcctgc accatccatt gcacgagctc gtcaaacgtt
      481 gtggccgcaa tcccttgcgg gcctggcgca gatgaagtct cgggtctgcc ggtcgggtgt
      541 tgaagcagag ggccttcctc tgcgctgcgt ttcgtcgaag cggacatccg caacaggctc
      601 acgccccggc agccaagcgc cacatgcgcc gccggcgtta accttcgggc ttcctggtaa
      661 acaaaaggta aaatcggtac gggtgggacg gtttttgatt cgtgctgtga ggtatttgcg
      721 tcaaaatatt aaggatttta cctttcgcac gggccgcgct cccgggcgac cggcgacccc
      781 ggcccgagcg ggttatttcc gggcgcgccg ttccgcctgc acgatctggt cgcgcagaaa
      841 atcggcaaag tcgatgccct gtgccttcag tgccttgggg tagagcgagg ccttggtcag
      901 gcccggcagc gtattggtct cgagatagat cggccccttg tccgagacga tgaagtcggt
      961 gcgcgaataa ccgctgcagg acagcgcgca gtgcgccttc aacgcttgat ccatgatcgc
     1021 cgcgctgatc tcgggcgaaa agcgtcccgg gcagatctcc tgtgtcgact tcagcagata
     1081 ttttgcggtg tagtcgaagg tcccctctcc cggcacgatc tcgaccggcg gcagcgaaat
     1141 caccgagccg tccgaccgct cgagaacccc gcaggtcgct tccacgccgg ggataagcgg
     1201 ctcgatcagg tagtcttcgg tcgcggcggc gttgcggacg gcgacgaggt cctgcctggc
     1261 gttgacgaag atcaggccgt aactcgagcc gtcacgcgtg ggcttggcaa tcagcttgcc
     1321 gtactcggcc agtgccgttt cgatctcttc cagcgcaaca cctttcggcg caacgacgcc
     1381 cgcaatctcc acaaagcgct ttgctgcgac cttgtcgaag gcaagattcg acgacgccga
     1441 gcccgagccc gtgaagggaa tgccgcgcat ctcgcacatt tcctgcagtt cgccgttctc
     1501 tgctctgccg ccatgcaggc cgagcacgag cacgcggccg tcgctctttg cctgatccag
     1561 cgcctgatcg atggtggagg cgatgcgccg ccccccgggc ttgaacgcat gctcgaaggg
     1621 gcgcgcatgc ccaaggaggg ttttcgattg tgtttcgtgc acgctgtcat cggcttccca
     1681 gaaccacagg tcggcctcgg gcaacgcctc gcacagcgcc tgcgcgcttg cgaccgacac
     1741 cagccgctcc ttgttggttc cgccgaacag aatggtgacc cgcatcatgt ccgctcgatt
     1801 ccgatgcgct tgatttccca atgtaactcg attccggacg tttctttgac acggttgcgg
     1861 accgtttcac ccaatgtctc aatatcatgt ccggtcgcat tgccggtgtt gatcaaaaaa
     1921 ttgcagtgca tttgcgagac ctgcgcgccg ccgacgcgca ggccgcggca gcccgctgca
     1981 tcgatcagct tccacgcgct gttgccgggc ggattcttga aggtggaacc gccggtcttc
     2041 tcgcggatcg gctgtgcggt ttcgcggtga cgctgcacct cctccatccg cgcgcgaatc
     2101 gcatccggtg cggccgcgac gccgcggaag cgcgccgagg tgaagacgat cgagggatcg
     2161 acgcccgagt tgcgatagac gaatttcatg tcggcgttgg agaaggtgtg gacggtgccg
     2221 ttgcggtcga tgccggtggc ctcgaccagc acgtctttcg tctccgcgcc gttggcgccg
     2281 gcattcatgc gcagtgcgcc gccgatcgtg ccgggaatgc cgaagtaaaa ttcgaggcct
     2341 gcgagattgg cgcctgccgc ggtctccgcc acgcgcctgt cgagcgcggc cgcgcctgcg
     2401 acgacgatgt cgccttggac agcggtttcg ccaaagcctc gcggcgagag gcgaaccact
     2461 atgcccggca tgccgccgtc gcgcacgatc aggttggagc caacgccgac gacatagacg
     2521 ggaagctctt gcggcagatt tttaaggaaa tacgcgaggt cgtcctcgtc ggaaggcgtg
     2581 aacaacactt gcgccggacc gccgacacga aaccaggtga gtggcgcaag gctttcattg
     2641 gcgagcagcc gcccccgcag ttccggcatc gcggccttca gttctggcgt gatgtcggta
     2701 aagggtgcca cgctatccca acgccttcaa ttcgccgggc agcgcgtagg cccattgcgt
     2761 gatgctgcct gcgccgaggc agacgacgag atcgccgcgg gttgcgaggc ccttgacgat
     2821 gccggcaaga tcggccggct tcggcagcgg gaccacctcg cgatggccgt gcgcgcgcaa
     2881 gccgaggacg aagtggtcgc ggtcgatgcc atcgatcggc gtctcgccgg cggggtagac
     2941 ctccgcgaca accaccgcat cggcgtcgtt gaagcaggtg caaaactcct cgaacagcga
     3001 ttgtagccgc gtgtagcgat gcggctgcac cacggcgatg atcttgccgt tggtagaccc
     3061 gcgcgccgcc ttcagcacgg ccgcgatctc caccgggtga tgaccgtaat cgtcgatgac
     3121 ggtgacgccg ttccattcgc cggtacgggt gaagcgtcgc ttgacgccgc caaaacccgc
     3181 gatcgccttg cggatggcgt catccgatag gccgagctga tgggcgaccg caatcgccgc
     3241 cgtggcatta agcgcgttgt gctgtccggg catcggcaac gtgatcccgg tgatctcgtg
     3301 ggcggcgtcc gtcttgcgat cgcggatcac gacgttgaat ttcgaccctg cgcccatggg
     3361 cacgagatcg accagccgcg cgtctgcctg cgggttctcg ccataggtga tgatgcggcg
     3421 gtcctcgatc ttgccgacca gcgtctgcac gacgggatga tcgatgcaca tcacggcaaa
     3481 gccatagaac ggtacgttct cgacgaaggc gcggaacgcg tcctggatcg cctcaaaagt
     3541 cccgaagtga tcgagatgct cgggatcgat gttggtgacg atcacgacat cggatggcag
     3601 tttcaggaac gtgccgtcgc tctcgtcggc ttcgaccacc atccagtcgc cggcgcccaa
     3661 tcgcgcgttg gagccatagg cgttgatgat gccgccgttg atgacggtcg gatcgaggcc
     3721 gccggcatcg agcaatgttg cgaccatcgt cgtcgtggtg gtcttgccgt gcgtgccggc
     3781 gatcgccaca cagcttttca gtcgcatcaa ttcggcgagc atttccgcgc ggcgcaccac
     3841 gggaatgcgc tgggcgcgcg ccgccgtcaa ttcgggattg tcgcgcttga tcgcggtcga
     3901 gaccacaacc acgtcggcgc cgtcgacgtt ttcggccttg tggccgaccg tgaccttgac
     3961 gcccctttcc cgcaatcgcg ctacgtttgc gctatcgacc gcatccgagc cctgcaccgt
     4021 atagccgaga ttgttcagga cctcggcgat gccgctcatg ccgatgccgc cgatcccgac
     4081 aaagtggatg ggtccgatct cgcgcggcag tctcatgctg tttccccagt tacgagttgc
     4141 cttattaaag cctgccatgg ccggaacaag tccggcgaca acgaaccgag cccgaaattt
     4201 cggcctctcg gcctaaattc cagcgatttt caccaccagg tccgccagcc gttcggccgc
     4261 gtcgagtcgg ccggcggtcc ttgcggatgc cgccatggcc gcgagcttcg cgggctcagc
     4321 ggcgagcgcc gagatttccg cggcgaggcg agcgggcgtg aactccgtct gcgcgatacg
     4381 caaaccgccc ccgacctttg ccagcacgcc ggcattcgcg aactggtcct gatcgatcga
     4441 accgggcagc ggcaccagaa tggacggccg gccgatcgct gcaagctcgg ccaccgtgcc
     4501 ggcgcccgag cgcgatacga tcagatggtt cgatgcaagg cgcgccggga ggtcagtgaa
     4561 gaacggcgcg agctcgcatt tgatcttgag gcgatcgtaa acgttgcgga cccgcgccat
     4621 gtcctcgtcg cgaacctgct gcgtgagcac gagcctgctc cataaggccg gctcgagccg
     4681 ctcgatcgcg ccgggcacga tgtcgctcat cacacgcgcg ccctggctgc cgccgaccac
     4741 cagcaggtgg aacggtgccg tcgcgtccgg cggtgcgtaa tttgtcgagg cggcggcaag
     4801 gatcggcgga cgcaacggcg tgccgacggc ggtcgccttt gcggcgagcg ccttgtcgcg
     4861 atcgaacacg ccgggcaacg aggtcgcgat cgcgcttgcg cggcgcgcga gaaagcggtt
     4921 cgcgcgcccg agtaccgcat tggcttcgtg aatgatgccg ggaatgccgc atagccgcgc
     4981 ggcgatcagc ggcggcaggg tcggatagcc gccgaagccg accacggctt ttggtttcag
     5041 gcggcgcatg agcctgaagg ccgtaaacgt gccgacggcg agcgtgatcg cggcacgagc
     5101 gagttgcagc ggattgcgtc cgcgcgcggt ctcgctcgcg accacgtcga tcatgtcctt
     5161 ggagaacagg ccgctgtagc gcagcgcgcg cgagtcggtg acgagacgaa cgtcaaagcc
     5221 gcgcttgatc agttcgacgc caagcgcctc ggccggaaac agatggccgc cggttccgcc
     5281 cgctgccagc agaatcatgg gttttgggtc catgggctct caatagccca tggcatggcg
     5341 ggcgtcacgc
//
LOCUS       DATLJT010000719         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_504534,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000719 DATLJT010000000
VERSION     DATLJT010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504534"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..284
                     /locus_tag="VM910_16420"
     CDS             150..284
                     /locus_tag="VM910_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64150.1"
                     /translation="MSVKERTILLYDTPDYVGERIEVKGSSANVAAKHWVEAARFRGN
                     "
     gene            complement(325..819)
                     /locus_tag="VM910_16425"
     CDS             complement(325..819)
                     /locus_tag="VM910_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64151.1"
                     /translation="MGLFRRKEPSYLIPVGPDNATNKDWVILLETSTIVGPSPDKLRV
                     ARSTLEKKIVKNYNILLRARYFLDVEGYDPRIHAVYFCPQLYKYDTDKALVPLEGEEI
                     GKLIAQALASGVKPRRPWYEPSEKLPDQPIINSPKEDFETLVVEMPTARTSSGKKPLM
                     VAAE"
     gene            complement(1169..2296)
                     /locus_tag="VM910_16430"
     CDS             complement(1169..2296)
                     /locus_tag="VM910_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI64152.1"
                     /translation="MGPLTGFRIVEFAGIGPGPMAAMLFADLGASIIRLDRLTPSGLG
                     IDKPMRFDLIARSRPSVAVDLKHPDGRALAADLIGEADALIEGFRPGTMERLGLGPEA
                     ALARNPRLVYGRITGWGQSGPLAQAAGHDLNYLALTGALAAIGRAGSKPTPPLNLVAD
                     FGGGALYLAFGMACAMVEAQRSGKGQVVDAAMTEGAASLMTSFYGLHAAGLHTLERGT
                     NLLDSGSAIYETYECADGRFVSIAPIELKFRKVLFELLGLPHTNDDGAELRGKLERLF
                     KTRTRDEWCSLLEGTDACFAPVLTMAEAPHHPHNVARGTFIDIDNVLQPAPAPRFSRT
                     PSGRPTPPHVSGEGSRSTLADWGIAGERVDALFACGALARE"
     gene            complement(2422..>3011)
                     /locus_tag="VM910_16435"
     CDS             complement(2422..>3011)
                     /locus_tag="VM910_16435"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64153.1"
                     /translation="RPGGNATGFMVFEYSMGGKWLELLRQIAPGVMRVAVFRDASIPS
                     GNALFGVIQVMAQALKVEVTPVNVRDADEIERVLAAFARAPGGGLIVTGSALAYNYSD
                     LIITLAARHKLPAVYYEHSVVAAGGLMSYGPDFVDQYRQAAGYVDRILKGEKPADLPV
                     QAPKKYELIINLKTAKALGLTVPPSLLASANEMIE"
BASE COUNT          624 a          890 c          861 g          636 t
ORIGIN      
        1 aaggctgccg ctttgcccaa gctgcgctca gatcatgagg ctcgctccga aagcgtcgcg
       61 atttggcgat ctgactggcc ttcgtacctt tgaatgtcga gcctgtggag tgtcgttgat
      121 cgaggcagca tgaacgaaca ggcgcggtga tgagcgtcaa agaacgcacc attctcttat
      181 atgacacacc cgactatgta ggcgagcgca tcgaggtcaa aggatcgagc gccaacgtcg
      241 ccgccaagca ctgggttgag gcagcgcgct ttagaggcaa ctagtctcaa ggggtgggtt
      301 gcgatggcca gtacgttcgg aactctattc tgcggccacc atcaatggtt tcttgccact
      361 cgaggttcgc gcagtaggca tttcaacaac gagggtttca aagtcttcct ttggactgtt
      421 gattattggc tgatccggca acttttccga tggttcatac catggtcgcc tcggtttaac
      481 tccactcgca agagcttgcg cgatcaattt gccgatttcc tctccttcta ggggcacgag
      541 tgctttatca gtatcatatt tgtaaagttg aggacaaaaa tatacggcgt gaattcgagg
      601 atcatatcct tctacatcaa ggaagtaccg tgctctaagc aagatgttat aattttttac
      661 aattttcttc tcaagcgtcg atcgggcgac ccgcaatttg tcgggggacg gaccaacgat
      721 cgtgctggtt tctaaaagga taacccaatc tttgtttgtg gcgttatccg gaccgaccgg
      781 aattagataa gatggctctt ttcgtcgaaa gaggcccata gacagttctc ctcagcctgc
      841 gctgccaaac acccagcccg acaacttgca catagcggtc aattaagggt taacagcgca
      901 cctcgaagat ttctcacaaa aatttacttc actccttaat cgaatcgcat tctttgtcgt
      961 tccggtcgtt gtggcactga ttcctgtagt tgggttcgcg ctctcgattt acagatggct
     1021 ctacccgctc ccaaagatct acgccagggg agtgacaccc attgcgccat tggcaggtca
     1081 agaaagctga tcctaaatcg cgaatttccc atgtccgctt tgtgccaaaa gcagactcat
     1141 gcaccgcagc aaacaggcgc tcgttaaatc actcacgggc aagcgcgccg caggcgaaaa
     1201 gcgcgtcgac tctttccccc gcaatgcccc agtcggcgag ggtggaacgc gatccttctc
     1261 cggacacatg cggcggtgtc gggcggccgg agggcgtcct gctgaagcgt ggcgcggggg
     1321 cgggctggag gacgttgtcg atgtcgatga aggtgccacg tgcgacgttg tgcggatgat
     1381 gtggcgcctc cgccatggtg agaaccggcg cgaagcaggc gtcggttcct tcgagcagcg
     1441 agcaccattc gtccctggtg cgtgtcttaa aaagcctttc gagtttgccg cgcagctccg
     1501 ctccgtcatc gttcgtgtgg ggcagaccga ggagctcgaa aagcaccttg cggaacttga
     1561 gctcgatcgg tgcgatcgac acgaaacggc cgtccgcgca ttcatacgtt tcgtagatcg
     1621 cggagccgga atcgagcagg ttggttcccc gctccagagt gtgcaaaccc gcagcatgaa
     1681 ggccataaaa gctcgtcatt agcgacgcgg cgccttccgt catcgccgca tcaacgacct
     1741 gcccctttcc ggatcgctgc gcctctacca tggcgcaggc catgccgaag gcgagataga
     1801 gcgcaccgcc gccaaaatcc gccacgaggt tgagaggcgg cgtgggcttg gagccagcgc
     1861 gcccgatggc ggcgagcgcg cccgtcaatg cgaggtaatt aaggtcatgg cccgccgcct
     1921 gagccagcgg acccgattgt ccccatcccg ttatccggcc gtaaacgagg cgcgggtttc
     1981 gcgccagcgc cgcctccggg ccaaggccca aacgctccat tgttcctggc cgaaatcctt
     2041 cgatcagcgc atcggcctcg ccgatgagat cggcggcgag agcgcgcccg tcgggatgtt
     2101 tcaggtcaac cgccactgaa ggacgagagc gcgcgatcaa atcaaaacgc atcggcttat
     2161 ctatgccgag gccgctcgga gtcagtcgat ccaatcggat aatgctcgcg cccaggtcgg
     2221 cgaacagcat agccgccatt ggcccgggcc cgatgcccgc gaactcgact atgcgaaaac
     2281 cggtgagagg tcccatcgtc gcttccgtct ttccgacctt gttttcgaag tcgaaactat
     2341 gtccgcttta ccgccaaaag cggacattgc cgggtgatgg ttggatgtcc gctttgtgcc
     2401 aatagcggac attgagccgt ctcattcgat catctcgttg gcactggcga gaagcgatgg
     2461 cggcacggta aggcccaacg ccttggcggt tttgagattg atgatcagct cgtacttttt
     2521 tggcgcctgg accggcagat cggccggctt ctcgcccttg aggatgcgat cgacatagcc
     2581 agccgcttgg cggtactggt cgacgaaatc cggcccatat gacatcaggc cgccggcggc
     2641 gactacggag tgttcgtagt acaccgcggg cagcttatgt cgcgccgcaa gcgtgatgat
     2701 taggtcgcta taattgtacg ccagcgcact ccccgtcacg atcaggccac cacccggcgc
     2761 gcgcgcaaag gccgcaagaa cgcgctcgat ctcgtcggcg tcgcgcacgt tgaccggggt
     2821 cacctctacc ttgagcgcct gcgccataac ctggatgaca ccgaacaggg cgttgccgga
     2881 gggaatgctc gcatcccgaa aaaccgccac ccgcatgaca cccggcgcga tctgcctgag
     2941 cagctccagc catttcccac ccatgctgta ttcgaaaacc atgaagccgg tggcgttgcc
     3001 gcccggccgc g
//
LOCUS       DATLJT010000720         1597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3031240,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000720 DATLJT010000000
VERSION     DATLJT010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3031240"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VM910_16440"
     CDS             complement(<1..642)
                     /locus_tag="VM910_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HVI64154.1"
                     /translation="MMYVHALMGAESTTGPPVIRTIGVMDLKDALARGMSDFSAMPTH
                     VFFLCLIYPIVGLILARVSFGYDVLSLLFPLAAGFALLGPFVAIGFYELSRQREQGLD
                     ISWEDAFDVLHSPSRDAIAALGFLLLVIFVLWVGVAEAIYIETFGHEPAASIPNFVRE
                     VFTTPAGWTLIIVGNGIGFLFALAVLIISVVSFPLLLDRDVGAVEAVLTSVRAV"
BASE COUNT          356 a          469 c          485 g          287 t
ORIGIN      
        1 gacggctcgc accgaagtca gcaccgcttc aacggcgccg acatcgcggt cgagcagcag
       61 cggaaatgaa accacgctga tgatcaagac cgcgagcgcg aacaggaagc cgatgccgtt
      121 gccgacaatg atgagcgtcc agccggcggg cgtggtgaag acttcgcgaa cgaaattcgg
      181 aatcgaggcc gccggctcgt ggccgaaggt ttcaatatag atagcctctg caacgccaac
      241 ccagagcacg aagatcacca gcagcagaaa accgagcgcc gcaattgcgt cgcgcgatgg
      301 cgagtgcagc acgtcgaagg cgtcttccca tgaaatgtcg agaccttgct cacgctgccg
      361 gctcaattcg tagaatccga tagcgacgaa tgggccaagg agcgcgaagc cggccgcaag
      421 gggaaacagc agggacaaga cgtcatatcc gaagctcacc cgcgccagaa tcagaccgac
      481 gatcggatag atcaggcaga gaaagaacac gtgggtgggc atggccgaga aatcggacat
      541 tccgcgtgcc agcgcgtcct tgaggtccat cacgccgatg gtgcggatca ccggcggacc
      601 ggtggtcgac tctgccccca tgagagcatg gacatacatc atgttggtgc ctcgcgggaa
      661 agaggagcac cgcgctgcgt cttatggccg cgacccgagc ccatattaac gcagaaaagc
      721 cgggattccc cgaatgactc ccgaactggc gtgaatattc gcgcttttcc ggcctcgtag
      781 caagactgac gcgaggtgtg gatggctacc agcagctcga gcagcccgaa cgctggtcga
      841 ggggctgatc tggctatttt cactggcatc tggacgcatt cctcctgtcg attgcgcttg
      901 aacgcgtgac aatcaaacgc ttcatgctgg cgcgggcggc ggctgcggcc gtagctccgc
      961 gagccggcgg ggcaggatcc cgaactgcta ctcacgcctg gaacggtcgc agtaccgctc
     1021 cgttcgcagc cgaatgccag tcctttctgg aaatggggta gtgtcctaat ttgccaaatt
     1081 aggactaccg gataatgttg ttgctggttt tgggcagact ggataatggg atgatccgca
     1141 tttggcagcc caatacggga gtagcttaga tgccggccac cgatccaatc cccagcgtga
     1201 ctgcggatca gcttactcgg gctttgggcg aggctgttat tcggatatgg agcaatctgc
     1261 ctcaagacgt gcaacttcat ctatttcagg aagcggtgac gtcccagggt gaatccataa
     1321 gagcgcagct tgcggtcttc ctgcgtgaca aggatccgcg cacttcggac ccattaggca
     1381 accctcgcga aatgacggag ccggacagcc ttgggggtta gcgcgattgc ccgccgcgtg
     1441 agtctcgcga tatgcaacgg cgcctgcgat cgcggtcctc tccgctacat acacggtcgc
     1501 gccgttgccc cccgcgatca agacccgccg gcgttgaccc cggtggacca gcggcaagat
     1561 taccccagcc acaaatagaa aacgttttct ggtccgt
//
LOCUS       DATLJT010000721         1802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4180486,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000721 DATLJT010000000
VERSION     DATLJT010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4180486"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VM910_16445"
     CDS             <1..198
                     /locus_tag="VM910_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64155.1"
                     /translation="GTSAAIVERLSLELRAAVMSEEVRERMLNEGAQPVASTPAEYAA
                     EIDSEERKWSALVKSLNLKME"
     gene            463..1471
                     /locus_tag="VM910_16450"
                     /pseudo
     CDS             463..1471
                     /locus_tag="VM910_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          360 a          540 c          574 g          328 t
ORIGIN      
        1 ggtacatcgg cggccatcgt cgagcggctg tcgctcgaac tccgtgctgc ggtgatgtcg
       61 gaggaggtgc gagagcgaat gctgaacgag ggcgcccagc cggtggccag cacgccagcg
      121 gaatatgccg ctgagattga ctccgaagag cgaaagtggt cggcgttggt gaagagcctc
      181 aatttgaaga tggagtgaca gcgttgccac tgtcgttaac cgggcgcgca gaccacgccg
      241 ctaacgacgg tcaatatcgg ccgcgagccg cgtcgtgttt gcttctggca ctaagcgggc
      301 ctgccgagac tatctgctgt ttgtccgctt ttggggcaaa gcggacgtgg gctgcgaacc
      361 cacgctgatg gtcctcgccg cttttgaccc tggctgtgtg aaaacgcatt tggttatttg
      421 attcgcgcta tatgattccg acgccatttg cggagggatc ggatgagacg cttcgttgag
      481 gggattgatc ggggccaagc gacgctgttt ccggaatgcc tggaagactg gatcgatgcg
      541 gacaatccgg ttcgggtgat cgatgccttt gttgagaagc tcgacttatc caggttgggt
      601 ttcgatggag tggcccccga ggcaacaggc cggccttcat atcaccccgc ggtcctgctg
      661 aagctttaca tctacggcta cctcaatcgg gttcagtcga gtcggcggct tgagcgtgag
      721 gctaagcgca atgtcgaggt gatgtggttg accggccggc tcattcctga tcacaagacg
      781 atcgccgact tccgcaaaga cagcgggcca gggatcaagc aagtctgtgt gcaatttatt
      841 gaactatgcc gcctgatggg gctgttgacg caaacgagcg ttgcgatcga tggcagcaaa
      901 ggcggtcaat acccgcgaca agaacttcac gcgcgggaag gtggagcgac gccgcgccca
      961 attggagaag agcgtggcgc gctatcttgc gcagctcgat acggctgacc ggcaagagcc
     1021 atccgaagag ctggcggcga agacggcgcg tttgaaggag aagctggtca agctggaaag
     1081 cgagatgcaa cgcttggcga tgatggagag gctgatgctg gcgtcacccg atcagcagat
     1141 ctcgctcacc gatcctgaca gccgctcgat ggcgaccagc ggacgcggct ccggcgtggt
     1201 cggctataat gtgcaggtcg cggtcgatac ggaacatcac ctgatcgtca cgcatgaggt
     1261 aacaaacgac ggctcagatc gggcacaact cgccaacatc gcttgccagg caaaggaagt
     1321 tctcggcgtc gatgaactcg aagccgttgc cgatcggggc tactacagca gtgaggaaat
     1381 cctcgcgtgt gacaaagccg gcattacggt aacgcttccc aaaccgatca cctctggcat
     1441 cgaggccaag agtcgcttcg gcaagcagga ccagccccat cgggccaaca tgccgccgcg
     1501 ccaggccgat gtcgggccac agccagtcgc ggttcctccc cgcgcgctgc cggcatcgcc
     1561 cgcggacgtt cagccgcagc cggtcgagac cagtccggcg atcggcgccg accttcagcc
     1621 gcgttctccc gacgtccggc ccagtccggt ggcccctgtc gacatccagc cgcggcctgt
     1681 caccgccgcc gacccgctgc caccgagagc cgacccgccg ctcgagatta gcgcccccca
     1741 cccgccgcct acggccgagc aggacaagga cgtgttctcc gccctcaagc gaattcccga
     1801 tc
//
LOCUS       DATLJT010000722         6810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2341894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000722 DATLJT010000000
VERSION     DATLJT010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2358)
                     /locus_tag="VM910_16455"
     CDS             complement(<1..2358)
                     /locus_tag="VM910_16455"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HVI64156.1"
                     /translation="MTTIEPNRNEAEQFLKALDPSPNARWCFQTFDDSKERKKERAEE
                     NKLRKKQGKRLLKDPLARWRYGTLAEHLDELVKLNERGAGIYFTVNETDGNGRKKTNI
                     TRIRASFVDLDGSPIEPVNNAEVKPHIVLESSPGRFHAYWCFTGRMSLKVFESLQKGL
                     AARFNGDPAIHDLSRVMRLPGFIHRKGEPFASHIVAINGSELHRASILLKTFRPARVK
                     KEEKPKQPGPQPQAGPQPPDDELRERWKKLNREAIRRYSDWVPHVFPGATKTSEGGYR
                     VSSANLGRDLEEDLSFHPDGIKDFGVHDLGDPRQGRRTPLDIVEQYQNKDFNEAVRWL
                     AQKLGLDPQDYLPKPKAKTNGQGSGDPATDAEIARLAKLSVVQYDRERKAAAEKLKVR
                     PATLDKLRSNERAKQAPAKAAPAAKTEAERIMAELNRDNCVVLDGARIRVLRFEEVEH
                     DAGGEHYVYRVPTFLRFEDFRYLYFNRHIAANGELMDIGKFWLTHEDRRQYPGIVFKP
                     GGEPIVNGKLNLWTGWGVTPRRGDWSLLGEHIYEVLAARDDDVYNYIINFLAWAVQHP
                     DQQPEVALVFKGERGTGRGTLGKVMCKLFGQHARHISSPAHLTGRFNAHLRQISFLFA
                     DEAYAPDDKSAEGTLKRLITEPTLTIEPKGRDVMEEPNRLHTMLASNEDWVVPAGAYE
                     RRYQVQEVANIHRQDPSWFGPIYEQLRSGGYEALLFDLLERDLGDWHPRDIVRTAALA
                     EQQERSLSPLDTWWLELLQTGVLAGADPLKPDHARSNRYEEEKEVG"
     gene            complement(2355..2783)
                     /locus_tag="VM910_16460"
     CDS             complement(2355..2783)
                     /locus_tag="VM910_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64157.1"
                     /translation="MDSKSRNPARVGRVEHNSPSHYAHSYTTKTPATQSKLTVKCREF
                     KPLIRNTLRGFAEVHIGELRLTIKDVAVHVKGGRCWAQVPAKPQVRDGALVKNDEGKV
                     QYIAMLAWDTRAVADVFSDAVVAAVRALVPDAFEADGGVS"
     gene            complement(3038..3916)
                     /locus_tag="VM910_16465"
     CDS             complement(3038..3916)
                     /locus_tag="VM910_16465"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HVI64158.1"
                     /translation="MAATTKISWADGTVNFWIGCTKVSDACEDCYAVPIAADMGVDWG
                     DDAPRHRTSEQIWRNPLRWNAMHDHGQTHMIERGKSVPVPLWTFGNSLSDFFDNKAPP
                     EWREEAWTKVVRPTPLLRWIFLTKRISNVLKMLPADWNDGRNYRHVGIVASVGNQEEF
                     DRDVPRLVALKSHGVRWVGLSIEPQLGPISIMGCPESRQLDWVINGGESAHLMPPRKY
                     FVAWARLLIQQCRALDVPFFLKQLGSLAFDGERRVYTKHPAGADADEWPADLRVQQWS
                     RLCENEERPVSQPTLL"
     gene            complement(4010..4480)
                     /locus_tag="VM910_16470"
     CDS             complement(4010..4480)
                     /locus_tag="VM910_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64159.1"
                     /translation="MASLFEAYPEDPVAMWIELYRQDELSGGYFRNAYSSGDIMWTMG
                     LSWSDDVGSRLDAEDRLSIDHARELVAMIEARPLTRQRIARHFLENMTDGVQKHPIAG
                     PIVSRFAEATGHELAPLLPPEFESLSQFLVDRRERLLAILRKSIALSEPLEISL"
     gene            complement(4576..4821)
                     /locus_tag="VM910_16475"
     CDS             complement(4576..4821)
                     /locus_tag="VM910_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64160.1"
                     /translation="MNRRDSRRAGAMTPDHGPIDPRIASAIKELFAKGLIVQDGERPD
                     GNGVMRPAYRIADGVDRSKLPAILDAHDERKNDPSTN"
     gene            complement(4818..5699)
                     /locus_tag="VM910_16480"
     CDS             complement(4818..5699)
                     /locus_tag="VM910_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64161.1"
                     /translation="MTRIPLDALIRRVSRIAEQRFNREGEIDPIWLVETVSGEQQIIV
                     SPVMAPSALATSDYKDKLAAKMRETFADLDVVRYARAAECWTVDAEMIEEQFAQHYAA
                     LGYTLANHPSRREVVLFEAEDGTEVLTAFRDIVRPAHGKPYLGKLGAIERPRGLYGRW
                     LGLLPSKGHAEALRERPQHNSTLLPRIRNSNELPDDVGRVFVTAVPDAPIQLVGRRDP
                     ETGELCVVGALGPPRKDIPAPSGDDLPSYIEVVTGPEAERLILSVHRWLTERAEAEGL
                     TIEQFMAKYHEQPETKP"
     gene            complement(5775..6041)
                     /locus_tag="VM910_16485"
     CDS             complement(5775..6041)
                     /locus_tag="VM910_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64162.1"
                     /translation="MQYQKLTADFQNDLAGSGLKAADKKAAASAASAGDHFYCYLGIV
                     PPSAAMTTGSVGFSANSCAGLHCQRLTFRAFGNKALFPKVSKQG"
     gene            complement(6136..6708)
                     /locus_tag="VM910_16490"
     CDS             complement(6136..6708)
                     /locus_tag="VM910_16490"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI64163.1"
                     /translation="MRSSHCARGKDSAGAGPFGYRACRSILLKHGEHNMTVIKEDAAE
                     WTGGCQCGAVRYQLLTPPEHACLCHCRMCQRASGQPFMAFASVRREDLRWTRGSPSTF
                     VSSNVAERNFCSACGTPLTYQRVESGSIAVTIGSLDAPEAVRPIEQFGIESELSWISG
                     LRALPAKRTEDWKREYSILHIDSRQYLPAR"
BASE COUNT         1187 a         2100 c         2096 g         1427 t
ORIGIN      
        1 cccgacctct ttttcctctt cgtagcgatt tgacctcgca tgatcgggtt ttagcggatc
       61 agcaccggcg agaacgccag tctgcagcag ctcaagccac caagtatcga gcggcgagag
      121 gcttctctct tgttgctcgg cgagcgcagc ggtgcgcaca atatcgcgcg gatgccagtc
      181 gccgagatcg cgctcgagca aatcgaacag caaggcctcg tagccacccg atctgagctg
      241 ctcataaatc ggcccgaacc agctcggatc ttgccgatgg atgttggcaa cctcctgcac
      301 ttggtagcgg cgctcgtagg caccggcggg gaccacccaa tcctcattgc tggcgagcat
      361 ggtgtgcagc cggttcggct cctccatcac gtcgcggccc ttcggctcga tggtcaatgt
      421 aggctcggtg atgaggcgct tgagtgttcc ctccgcgctc ttgtcgtctg gggcgtaggc
      481 ttcgtcggcg aacaggaagg agatttgccg taggtgagca ttgaagcgac cggtgagatg
      541 cgcgggcgag ctgatgtggc gcgcatgctg gccgaagagc ttgcacatca ccttgcccag
      601 cgtgcccctg ccggtgccgc gctcgccctt gaacacgagc gcgacctcag gctgctggtc
      661 gggatgctgc acggcccacg ccaggaagtt gatgatgtag ttgtagacgt cgtcatcgcg
      721 cgcagcgagg acctcgtaaa tgtgctcgcc cagcaaagac caatcgccgc gcctgggcgt
      781 cacaccccag ccggtccaga ggttgagctt gccgttgacg atgggctcac cccctggctt
      841 gaacacgatg ccgggatact gccgccgatc ttcgtgcgtg agccagaact tgccgatgtc
      901 cataagctcg ccgttcgccg cgatgtggcg attgaaatac aaatagcgga agtcctcgaa
      961 gcgcagaaat gtcggcacgc ggtagacgta gtgctcaccg ccggcatcgt gctcgacctc
     1021 ttcgaaccgc aacaccctga tgcgtgcgcc gtcgagcacg acgcagttgt cgcggttgag
     1081 ttcggccatt atgcgctcgg cctcggtctt cgccgctggt gccgctttcg cgggggcttg
     1141 cttggctcgc tcattgctcc tcagcttgtc gagggtggca ggtcgcacct tcagtttttc
     1201 ggcggcggcc ttgcgctcgc ggtcgtactg aacgactgag agtttggcta agcgcgcgat
     1261 ctcggcatcg gtcgccgggt cgccgctgcc ctgcccgttc gtcttcgctt tcggcttggg
     1321 cagatagtct tgcggatcga ggccgagctt ctgcgccagc cagcgcacgg cttcgttgaa
     1381 atccttattc tgatattgct cgacaatgtc gagcggcgtc ctgcgccctt gacgcggatc
     1441 gcccaggtca tgcacgccga aatccttgat gccatccggg tggaacgaca ggtcttcttc
     1501 gaggtcgcgg ccgagattgg ccgacgatac gcgatagcct ccttcgctgg tcttggtcgc
     1561 gcctggaaag acgtgcggca cccaatccga gtaacgccgg atcgcttccc tattgagctt
     1621 cttccaccgc tcgcgtagct cgtcgtcggg cggttgcggc ccggcctgcg gttgcggccc
     1681 gggctgcttg ggcttctctt ccttctttac ccgcgctggt cggaacgtct tcagcaggat
     1741 agatgcccgg tgtagctcgc tgccgttgat ggcgacgatg tgcgacgcga acggctcgcc
     1801 cttacgatga atgaaaccag gcaaccgcat cacgcgcgag agatcgtgga ttgctggatc
     1861 gccgttgaag cgggccgcga gccctttctg tagcgactca aaaactttca gcgacattct
     1921 gccggtgaaa caccaatacg cgtgaaagcg gccgggcgag gattccagca cgatgtgcgg
     1981 cttcacctcg gcgttgttca ccggctcgat cggtgagcca tcgagatcga cgaacgacgc
     2041 acggatgcgg gtgatgttgg tctttttgcg cccgttgccg tcggtctcgt tgacagtgaa
     2101 gtaaatgccc gcgcctcgct cgttgagctt gaccagttca tccaagtgtt ctgctagcgt
     2161 gccatatctc catctggcaa gcgggtcctt cagtagtcgc ttgccttgct tcttgcgcag
     2221 cttgttctct tcggcgcgct ccttcttgcg ctccttggaa tcgtcgaacg tctgaaaaca
     2281 ccaacgggca ttcggtgacg ggtcgagcgc tttgagaaac tgttccgctt cgttgcggtt
     2341 gggttcgatg gtcgtcatga cacgcccccg tcggcctcga aggcatcggg gacgagcgcg
     2401 cgtaccgccg caacgacggc atcgctgaag acgtcggcaa ccgcccgcgt gtcccacgcg
     2461 agcatcgcga tgtattgaac tttcccctcg tcgttcttga ccagggcgcc atcgcggacc
     2521 tgcggctttg ctggtacctg cgcccaacaa cggccacctt tcacatggac cgcaacgtcc
     2581 ttgatggtca ggcgcaattc gccgatgtgg acctcggcga agccgcggag cgtgttgcgg
     2641 atgagcggct taaactcccg gcacttgacc gtgagcttcg attgcgttgc gggggtcttt
     2701 gtagtatagc tgtgggcgta atggctcggc gaattgtgct caaccctgcc caccctggca
     2761 gggtttctgc ttttcgaatc catgattggc ggctcggcac tggcggccgc gagaacattc
     2821 acgcgtaggc ggcggcttct gctatgcgtg attcccgtgc tcgagggatt tcgccatgct
     2881 cggccatgaa cccgaagcgt ctgatagctg ccatgctcgg cacagacggc cgccggggtt
     2941 agtccattga gactaacacg agtctgcgcc cgccccaaga aggcggatcc gccgatcgtg
     3001 cgggcatcag gtttttccac gtccgcgggc tcatccctca cagcaaggtc ggctggctga
     3061 cgggtctttc ctcgttttcg caaaggcgcg accattgctg cacgcgcagg tcggccggcc
     3121 attcgtccgc atcggcgcca gcgggatgct tggtatagac gcggcgctcg ccgtcgaatg
     3181 ccagcgagcc gagctgcttt aggaaaaaag gaacgtcgag cgcacggcac tgctgaatca
     3241 acagccgagc ccacgcgacg aaatacttgc gcggcggcat gagatgcgcc gattcgcccc
     3301 cgttgatgac ccaatcgagt tggcgagatt cggggcagcc cataattgag atcggcccga
     3361 gttgcggctc gattgagagc ccgacccaac gaacgccatg cgatttcaag gcgacgagcc
     3421 ggggcacgtc gcggtcgaat tcctcctggt tgcccacgct cgcgacaatc ccgacgtgac
     3481 gatagttccg gccgtcgttc caatccgccg gcaacatctt gagcacgttc gagatgcgct
     3541 tggtcaggaa tatccagcgt agcagcggcg tcgggcggac gactttcgtc cacgcttcct
     3601 cccgccattc gggcggcgct ttgttgtcga aaaaatcgga aagcgaattc ccaaatgtcc
     3661 agagcggaac gggcacggat ttgcccctct cgatcatatg ggtctgcccg tggtcgtgca
     3721 ttgcattcca gcgcaatgga ttgcgccaga tctgctcgct ggtgcgatgc cgcggcgcgt
     3781 cgtcgcccca atcgacgccc atgtcggcgg caatcggcac ggcgtagcag tcctcgcagg
     3841 cgtcgctgac cttagtgcag ccgatccaga aattcaccgt tccgtcggcc caagagattt
     3901 tggttgtggc cgccatgagt caccgcgctt cgggctcgtc gaggggtgtc cgaaagaacc
     3961 gcaacggctg accgccgatg gtcgcgatgt gcagcgggtt gatcatgcgt cagagcgata
     4021 tttccagcgg ttcgcttagc gcgatggatt tgcgcaggat ggccaacaat cgctcgcgcc
     4081 tgtcgacgag gaattgcgat aaggactcga actccggcgg caacaacggc gccagctcat
     4141 ggccggtggc ttcggcgaag cggctgacta taggccccgc gatcggatgc ttttgcacgc
     4201 cgtcggtcat gttttcgagg aaatgccgcg caatgcgctg gcgcgtcagc ggccgcgcct
     4261 cgatcatggc gacaagctcg cgtgcatgat cgatcgacag gcggtcctcg gcgtcgagcc
     4321 ttgatccaac atcgtccgac cacgaaagcc ccatcgtcca catgatatcg cccgagctat
     4381 aggcgttgcg gaaatatccg ccgctaagct cgtcctgtcg gtacaattcg atccacatgg
     4441 ccacaggatc ctcggggtaa gcttcaaaaa gtgaagccat ttgttgctct acggggttgg
     4501 cctcgcagta cggccgccag atcgagtgga gatagatgtc aacgcccatg tgtgagcccc
     4561 tcaagtcggg taggcttagt tcgtgctcgg gtcgttcttt cgctcgtcgt gagcgtcgag
     4621 tattgccggc aatttgctcc gatcgacgcc gtcggcgatc ctgtacgccg gccgcattac
     4681 gccgttgccg tccggtcgct cgccgtcctg gacgatcagg cctttggcga agagttcttt
     4741 gatcgcactg gcaattcgtg gatcgatagg gccgtggtct ggcgtcatgg ccccggcgcg
     4801 gcgggaatcg cggcggttca tggcttcgtc tccggttgct cgtgatattt cgccatgaat
     4861 tgctcgatcg tgagcccttc ggcttcggcc cgttcggtaa gccagcgatg cacggagaga
     4921 atcagccgct cggcttcggg gccggtcacg acctcgatgt aggacggcaa atcgtcgccg
     4981 cttggcgccg ggatgtcctt gcgcggcggc cctagggccc cgacaacaca tagctcgccg
     5041 gtttccggat cgcgtcggcc cacgagttga atcggcgcat ccgggacggc ggtcacaaac
     5101 acgcggccaa catcgtcggg aagttcgttg ctgttgcgta ttctcggcaa cagcgtggag
     5161 ttatgctgtg gccgctctcg cagtgcctca gcgtgcccct tactcggcag taagcctagc
     5221 catcggccgt agaggccgcg tggccgctcg atcgcgccga gtttgccgag atagggcttg
     5281 ccatgagcgg gccgaacgat gtcgcggaag gcggtcaaga cttcggtgcc gtcttcggcc
     5341 tcgaatagca cgacttctcg acggctcgga tggtttgcga gcgtgtagcc caaggcggca
     5401 tagtgctgcg cgaattgctc ttcgatcatc tccgcatcga cggtccagca ttccgctgcg
     5461 cgcgcgtagc gaacgacatc gaggtccgcg aaggtttcgc gcatcttcgc ggcaagcttg
     5521 tccttgtaat cggaggtggc cagcgccgat ggcgccatga cgggagacac gatgatttgc
     5581 tgctccccgc tcacggtctc gaccagccaa attggatcga tctcgccctc gcggttgaag
     5641 cgctgctcgg cgatgcgcga cacgcgccgg atgagggcat cgagggggat gcgggtcatg
     5701 gcttctcccc gtccggcggc ttgggcacga cttcgtgcgg ctcgcacacg tgttttgcct
     5761 gctcgtcctc gggctcagcc ctgctttgac actttcggaa aaagcgcttt gttgccaaag
     5821 gcgcgaaatg tgagcctttg acagtgcaag cccgcgcagc tgttcgcaga gaaacccacc
     5881 gacccggtag tcatcgccgc cgacggcggc acgataccca aatagcagta gaaatgatcg
     5941 ccagccgatg ccgcgctcgc ggccgctttc ttgtccgcag ctttcagccc ggatcccgct
     6001 aagtcatttt gaaaatcggc cgtaagtttt tgatattgca cgcgccgaat tgccctgcct
     6061 tttgaaaaaa agcgcagagg aatcagtgct ggcgggcgga ctcatgtacc gaagcagaaa
     6121 agcaacctta ctcgatcacc tcgccggcaa atactgacga ctgtcgatgt gcaggatgct
     6181 gtactcacgt ttccagtctt cggtacgctt ggcaggaagc gctctgaggc cactgatcca
     6241 ggacagttcg ctctcgatgc caaactgctc tatcgggcgt acagcttctg gggcgtccag
     6301 gctgccgatt gtcacggcga tgctgccgct ctccacgcgc tgatacgtca gtggggtgcc
     6361 gcaggcactg caaaagttcc gctctgcgac gttcgagctc acgaaggtcg acgggctccc
     6421 gcgcgtccag cgcaggtctt cgcgcctgac actcgcgaac gccatgaatg gctgaccgga
     6481 tgccctctgg cacattcggc aatggcaaag gcacgcgtgc tccggcggtg tgagcagttg
     6541 ataccgcaca gctccgcatt gacagccacc agtccactcg gccgcgtctt ccttgattac
     6601 agtcatgttg tgttccccat gtttcaagag gattgagcga cacgcgcggt agccaaaggg
     6661 ccctgcccca gcgctatcct tgccgcgcgc gcagtgcgac gagcgcatcg aataccaccc
     6721 ggtcgatctc caacccctcg tgaagtgcgc gctcgcgaga gtgaaatgcc cgctcggacg
     6781 gaatgcggat gtcgtcgacg ccgggccgcc
//
LOCUS       DATLJT010000723        14192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_45046,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000723 DATLJT010000000
VERSION     DATLJT010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45046"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..4681)
                     /locus_tag="VM910_16495"
     CDS             complement(227..4681)
                     /locus_tag="VM910_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI64164.1"
                     /translation="MAASDPHIPNELPDLRRVGRIAIDSEERDNGLAADRGSSWPWRD
                     GHVCGISVAYRADGQVRGHYFPMRHPDTQNFPPEQVYAWLKDHVASELSFITHNGPFD
                     WGWFRAEAGIRVPSGERLEELGALATLTNENRHGYGLDALARSCGLPGKDERLLKEGC
                     LALDLIANKRKKFRPQNYLWQLPAKYVAPYAVTDAINTLLTYELFDPILDQENTRAAY
                     RLECNLVPLIIEMQLRGIRIDIPAAERARDFLLGKRDAALNQLSDKLGMPVSMDELNQ
                     NDWKVEVCDREGVAYPRNEKGNPSFAKDWTEGHPHWLPQLIREAGKYHRVGDLFVDKF
                     ILGHIVNGRIHAEIHPFKTEDHGAKSFRFSYSDPPLQLMTARDEELAPIIRGLFLPDE
                     GEIWAKPDASQQEFRIAVHYAALHNMPGAELALQRYHDDPDTDFHLFAALMAGIDRPD
                     AKTFNFGFIYGMGIRTTAKKLGRSLKEAQKIYGQYNRALPFLRALSKAYERIARQQGH
                     ITLHNGARRHFNLWVPAGKWSKGAGPCDHEEAERRLADPKHKWSKIKNKQLYRAGIKD
                     ALNALIQGTAAIHTKLWMHACWREGVVPLLQMHDCLDCSVATREQAEMVAQLCVEAVE
                     LKVPMRCDLKYGRNWGDAKHTWEELHGEAAPAPVSVSVPAATTELEPTPIPEPEPELE
                     IESKIEPKFEPEPAAAQGEEAVMGAYAQCGEILIQRGYAAVPIMLGTKAPGFFCAGLW
                     MPLPAWQRRFLNKIPSERDHKLWSNGETGVGVVGGRASHGLIAFDTDTDDCAIKTALM
                     NVLPKTPVRKVGAKGETAFYYGPDITASRSWTIGGKRICDLIADGRQTVLPPTIHPDG
                     APYRWLEQPLEAFKPEELPRVSADMLEQIDAVLQTFGWQAEASRPASGDLGDTDDASP
                     HRQLNNFALAHLPRWVPRLRLYKCRPARGGYEAVAHWRESSSGRELEQRARNLSIVPK
                     GIKDFGDGRNGGAGFTYTPLDLVMAASDCDLDTAFKFLSQATGWAGEAWTESPEQATS
                     EPVSEAPIVAKAPAAEKGPATEPAEPEKKTAIDELEPYTHDVPGVVGEVTEWIVATAR
                     RPNRVLALAAAIPLVGTLIGRRVAGPTWSATHLYVVIAAPTGAGKQHPIDCIIALLTA
                     ADAQAHFGPGSFMSASALCNFISRRPLSLCCADEFGAYLAKLQAKGASGHEREVTKVM
                     RTLWGTSFTIAAMPEWADRIGEQIHSPALSFFGTSTPDELFQALQGEAIENGLLNRFL
                     VLRSNMRCWDTNPQLPTKEVPADLAMRCRQLYHWYGTDAELIDIGRLVPQQVTQLPWA
                     DKTAEQQYLDFAHAIDDRIDQDPTLYPFFARTVETAVRLATIRAAGQGFREAKITFED
                     IRWGTRIAEISARQAYLGAGGVIPTNERIRWINRLLSYVRARNLESKPATVRGFQQHI
                     RCALKAAEVRDMVAQLVQTGYLEQKWRIDLHTEAGGIAHTRAKKHAQR"
     gene            complement(4709..4954)
                     /locus_tag="VM910_16500"
     CDS             complement(4709..4954)
                     /locus_tag="VM910_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64165.1"
                     /translation="MTKPDASKQHKPGGGIIGYLDAESYGDGPFCWNCELTFKSLEDA
                     PVDLPVHFCTPLCAREWYSLQPLQNLLDKRRLRRERG"
     gene            complement(4973..6625)
                     /locus_tag="VM910_16505"
     CDS             complement(4973..6625)
                     /locus_tag="VM910_16505"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI64166.1"
                     /translation="MLILRQYQEKALRELFVFWRNGGGNPLIAMATGTGKSIVIAFLI
                     KQLLTDYPKMRVLVTAPNRELLEQDIRELQNIWPEAPIGINCDELGYRDTDAQILFAT
                     INSIYRNPDAIGERHLIIIDEGHLIPHGEQGMYHTTIGALRNHAPDLRVVGLTATPFR
                     LDSGHLCEGDGHLFDKVVFEYTIGEGIHDDWLAPLSSKTTHTTIDVSGVGKRGGEYIS
                     EQLEAAAIRNDAVELACDELASYQDKRRAWLVYCVGVTHAGLVRDALRARGVHTEMVL
                     GETPDQERDHIIEDFRAGRLTALVSVNVLSYGFNVPHIDLIAMLRPTCSAGLYIQQVG
                     RETRKADGKANCLVLDFAGNVRRFGPVDNVYIKHKNSRDGEAPTKTCPECDEIVLLAA
                     TECPSCGHQFSRREEPKHAAYADRVAILSPPRAVSDWLEVEDIEYRYHHKETPSLRVT
                     FQCGAQSFAKWVCLQHNGYARSQAEQFWRVLSGGLSVPHTVDEALNRQDELAWVSHIR
                     VAPEGEKYWRIIGYRIAGENYDGNLRRAIAWGRPEINDSIQY"
     gene            complement(6698..7255)
                     /locus_tag="VM910_16510"
     CDS             complement(6698..7255)
                     /locus_tag="VM910_16510"
                     /inference="COORDINATES: protein motif:HMM:NF016896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HVI64167.1"
                     /translation="MVDGYYDQLPEMFDPAAHEGTQDLVAVPAGWYLAHIVEAEVRDA
                     ANGNGNYLWTAFEIIEGQYKGRKVFHNVTIHNASQQAVEIGQRLMTDIYIACGITGPT
                     RSIDVLLHKPIKIRVNIRRDPNGEYSDKNRVTAARPYDFEPKRRGPGAAPVANPGPKP
                     ANAPEAMATASATPKGDRPWNQKQT"
     gene            complement(7258..8082)
                     /locus_tag="VM910_16515"
     CDS             complement(7258..8082)
                     /locus_tag="VM910_16515"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI64168.1"
                     /translation="MKPFQVTATLLPIVVMHGAPGIGKTTTAAKFPNPVFIQTEDGCP
                     SGLTISSFGLCDSFASVIEALTWLGKETHDYQTLVIDSLDAPEPLVQAALCADRGYAS
                     VESPGFGKGYVELDKYWLDFLRGCNWLRRQRNMIIVMIAHSEIIMINDPRTTAYSSYA
                     LRLRKRARGLVEDNADLIGFLATDIIIKNEQGGFGKTRAQADGGNTRWLHCDPRPAFT
                     SKNRFGMPERIQVPLNFNYQTMLGKFFPTPQPQAESMRTTAEPKTAIETEIGKQGG"
     gene            complement(8318..8782)
                     /locus_tag="VM910_16520"
     CDS             complement(8318..8782)
                     /locus_tag="VM910_16520"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HVI64169.1"
                     /translation="MTETITITVNGEVVAKGRPRVTRRGFVYTPAATRKYEAHARLAA
                     QLAMNGKSPITTPVRAEITIDLPVPTSWSGKRQDAALRGDVRPTTRPDADNFVKVGLD
                     CINNIVISDDSLVVDLIVTKKYAAVPALTIVIAPLTAATAQGRLAAVAEITA"
     gene            complement(8779..9315)
                     /locus_tag="VM910_16525"
     CDS             complement(8779..9315)
                     /locus_tag="VM910_16525"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HVI64170.1"
                     /translation="MKSQNETPMRAAVKMHEPSIGETSDWFTPPEYFDALGLEFDLDP
                     CSPGPGHWAPAKKIYTIADNGLRQPWHGLVFMNPPFGGRHGHIPWLVKFLDHANGIAI
                     VRAYTSSGWFHQYVFPRAELLLFPKGKTKFVRPDGSIGTAPGHGVVLIGMGEVACTAL
                     LRSGLGFCASIVSETVRP"
     gene            complement(9312..9914)
                     /locus_tag="VM910_16530"
     CDS             complement(9312..9914)
                     /locus_tag="VM910_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64171.1"
                     /translation="MLKRTDRRPLLAHVWTKDPHGFYIEPEWCSRRLFEVERFDGVIK
                     DPACGIGRITDAARAAGYRVIATDLVDRGYLQFVDAEDFLRSECRVDNVITNGPYHIC
                     RAFAEHALKLARRKVAMIWLARRLNAARWLVNTPLARVYLMSPRPSMPPGHVILAGEE
                     PGGGTQDFVWLVFSRGHRGSPELRWLHRDDDRGGRRESAA"
     gene            complement(10182..11408)
                     /locus_tag="VM910_16535"
     CDS             complement(10182..11408)
                     /locus_tag="VM910_16535"
                     /inference="COORDINATES: protein motif:HMM:NF022744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HVI64172.1"
                     /translation="MDHDGLNSGTLQKINGEAPQGVAATKRTPLDNPFLAEHAVAIRA
                     LGKQTAENIVEIGRRLVECRDRHLDHGQWLPWLEREFQWSRQTADNFIHVYEARAKLP
                     KFGNLSLPLSGLYLLTAPSTPEPAKTEIIERAEAGESLPVAEVKRVVKRHKGTKHHKQ
                     PTTPPPPPPAPTPARNDIGATSADNGVASTGEVGWLKARNEELENKCRLLELKIEGLE
                     SENSELKAENVALRVKLEVAQKAAAASPKIAATDTVTAGDPGPIPEFLQRKPAAAGTS
                     ESGGDVTPQDAVANAFDEIEDLACGCRGQVDNAPAGLDQSARIQALDETAGVLESLSM
                     PDVPAALGGIKVKLPKPRRLRSRGAQRDAALGIISACAEALDAIDESDPRHQEANDFC
                     SELQSASSELEGCEFS"
     gene            complement(11492..11845)
                     /locus_tag="VM910_16540"
     CDS             complement(11492..11845)
                     /locus_tag="VM910_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64173.1"
                     /translation="MAPLVTMSRLLRLRSYYTVPQAGAQIGLGRTGSYDAAEAGLIPV
                     ERAGAGGKLMLVPRNKWDRQVKRLLLRAAKPPRSTAPLAISVTLRNKPPSPRKRSVPR
                     RAHIASGAERGLRSS"
     gene            12007..12447
                     /locus_tag="VM910_16545"
     CDS             12007..12447
                     /locus_tag="VM910_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64174.1"
                     /translation="MVRKQVSDPSEHELEFKIKLPADIGERIRAKAQREGRPLNRVIT
                     DELREYPVLKETGTLAAAVDALAEQNATLAEQTREMKISLARHGARIDWLDLTDELVN
                     AIDLVNEALETDSPSLPAAMDALRAGLLTARTLGPEVKRKLKKP"
     gene            12470..12736
                     /locus_tag="VM910_16550"
     CDS             12470..12736
                     /locus_tag="VM910_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64175.1"
                     /translation="MVTGDSIRFSVVPRDVPAAKAARRLHLTLNEFEEMKDELFRRGF
                     PRPDPTTGNFDLVEIDRWMDGRHHHCDSNKPRDARELLPPGLRR"
     gene            12733..13923
                     /locus_tag="VM910_16555"
     CDS             12733..13923
                     /locus_tag="VM910_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64176.1"
                     /translation="MTTDKIRYLRFLNGQWRWRPEAYMRAYGFKLTTLGPEKTASVIA
                     RAAALNEEWDRVKRGAALAAQSIAIKYPPGTIGDGYTRAMESRAADRAEAGKPQTKDQ
                     EKRDDWKRAWKHLERWALYDPTTIQPEHFLAIDPVTGQTVGLIADLNRVSAGERHRTI
                     KVWRALWKRMARLGYCALEADPSKVRPNSAPQPRQAVWMHREVMKLVQGAWRSGKRGL
                     ACFIAITWDTMLSPVDARALTLAQMRERSFFTVARAKTGRAALATLSPYSQALLAAYL
                     GPVQPLNDAPIFRTAGSEPGPKGGRRWTPVPYTKDKLEKDFAEVRALVFGPAERRQMS
                     DLRRSGHVEGDAGGATPVDASAKMANTIAVSNRLHKTYNPVNIASVQRFDEARKVGRG
                     KLKG"
     gene            complement(14054..14130)
                     /locus_tag="VM910_16560"
     tRNA            complement(14054..14130)
                     /locus_tag="VM910_16560"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14094..14096),aa:Asp,seq:gtc)
BASE COUNT         2696 a         4240 c         4208 g         3048 t
ORIGIN      
        1 ttgtataccc ctatatgtgt ccaccgtgtt tcctcgctta agttagcctc aggatcaggg
       61 tcattctttc tttactacat acaacacaca cacaaacaca cacactacac ttaatataga
      121 tagaatgggc attttcgaaa attttcgacc aatcagacaa gctttcataa tgccttcatg
      181 cagacgcaaa aaatcctaca acttttcgat tttagctccc tgctaatcag cgctgtgcat
      241 gttttttcgc ccgtgtatga gctattccac cagcttctgt gtgcagatca attctccatt
      301 tctgctcgag ataaccagtc tgaacaagct gggcgaccat gtcgcgaact tcggcagctt
      361 tgagcgcaca tcgaatgtgc tgttggaacc ctctcacggt agctggcttg ctttccagat
      421 tccgggcacg gacgtagcta agcaaccggt tgatccagcg aatacgctcg ttagttggaa
      481 ttacacctcc ggcaccgaga tacgcctgtc ttgccgaaat ctcggcaatt ctggtgcccc
      541 atcgaatgtc ctcgaaggtg atctttgcct cccgaaaccc ctgcccggcc gcacgaatag
      601 tagcgagccg cacagcggtc tcgaccgtgc gcgcgaagaa cggatagagc gtcgggtctt
      661 gatcgatccg atcatcgatt gcgtgagcaa aatcgagata ttgctgctct gccgttttgt
      721 ccgcccatgg caattgcgtt acctgttgcg gaacgaggcg tccgatgtcg atgagctcgg
      781 catcggtgcc ataccagtgg tagagctgcc ggcatctcat cgcgagatcg gccggtactt
      841 ctttggttgg aagctgcgga tttgtgtccc aacagcgcat gttcgagcgc agcaccagaa
      901 atcgattgag taagccgttc tcgatcgcct cgccttgtaa tgcctggaac agctcgtcgg
      961 gcgtgctggt gccgaagaag ctcaaagccg gcgaatgaat ttgttcccct atacgatcgg
     1021 cccattcagg catggctgca atcgtgaacg atgtgcccca cagggtgcgc atgaccttgg
     1081 tcacctcgcg ctcgtgtccg gaagcaccct tggcctggag cttggcgagg taggccccga
     1141 actcgtccgc gcagcacagc gacaacggcc ggcgcgagat aaaattgcac agcgccgatg
     1201 ccgacatgaa gctgccagga ccaaagtgcg cctgtgcgtc ggccgcagtc agtagtgcga
     1261 tgatacaatc gattggatgc tgcttaccgg caccggtcgg cgcagcaatg acgacataga
     1321 gatgagtcgc cgaccacgtc ggtcctgcca cacgacgccc aattagcgtg ccgaccagcg
     1381 ggatcgccgc tgccagggca agcacgcgat tgggccgccg tgccgtggcc acgatccact
     1441 cggtcacctc acctaccacg cctggcacgt cgtgcgtgta aggctcgagc tcgtcgattg
     1501 ccgttttctt ctcgggttcc gccggctccg tggcgggccc tttctcggcc gctggcgctt
     1561 tcgccacgat aggcgcttct gagacaggtt cagatgtcgc ttgttctggg ctttcggtcc
     1621 aagcctctcc ggcccagcca gtagcctgac taaggaattt gaacgcggta tcgaggtcgc
     1681 agtcgctggc cgccatcacc agatcgagcg gtgtgtaggt aaaaccggca ccgccgttgc
     1741 ggccgtcacc gaagtccttg atgcccttag gcacgatgga gagattacgg gcccgctgct
     1801 cgagctcgcg accgctcgag gactcgcgcc agtgcgcgac cgcttcatag cccccgcgtg
     1861 cgggccggca cttatagaga cgaagtctgg gcacccagcg ggggagatgc gccaaggcga
     1921 agttgttgag ctggcggtgc ggactggcat cgtcagtatc gcctaggtca ccactagctg
     1981 gtcgcgaagc ctcagcctgc cagccgaagg tttgcagcac ggcgtcgatc tgctcaagca
     2041 tgtccgcgga cacacgcggc aattcctccg gcttgaacgc ctcgagtggc tgctcgagcc
     2101 agcgataggg cgccccatca ggatggattg tcggcggcag cacggtctgc cggccatcgg
     2161 cgatgaggtc gcagatgcgc ttgccaccaa tggtccagga ccgcgaggct gtgatatccg
     2221 ggccatagta gaacgccgtc tcgcccttgg cgccgacctt tcgcaccggc gtcttgggca
     2281 gcacgttcat cagcgcggtc ttgatcgcac aatcgtcggt atcggtgtcg aaggcgatca
     2341 gcccatgcga ggcgcgcccg ccaacgacgc cgacaccagt ctcgccgttg ctccatagct
     2401 tgtggtcgcg ttcgctcgga attttattta gaaagcggcg ctgccacgcc ggcaacggca
     2461 tccataatcc ggcgcagaag aagcctggcg ccttggtgcc gagcatgatc ggcaccgcgg
     2521 catagccgcg ctgaattaat atttcgccgc attgcgcata cgcacccatc accgcctcct
     2581 cgccttgtgc tgccgctggc tcgggttcga atttgggttc gatcttggat tcgatttcga
     2641 gttccggttc aggttcgggg ataggggtcg gttcgagttc agtggtcgcc gcaggcacag
     2701 acaccgatac aggcgcaggg gctgcttcac cgtgcagttc ctcccaggtg tgcttggcgt
     2761 caccccagtt gcggccgtac ttgagatcgc agcgcatggg gactttgagt tcaacagcct
     2821 cgacacagag ttgcgccacc atctccgcct gctcgcgtgt cgccaccgag caatcgagac
     2881 agtcatgcat ttgcaagagc gggacgacgc cttcacgcca gcaggcgtgc atccagagct
     2941 tggtgtggat tgccgccgta ccttgaatga gggcgttcag tgcatctttg atgccggcac
     3001 gatagagctg cttgttttta atcttggacc atttgtgttt tggatcggcg agccggcgtt
     3061 cggcttcttc gtgatcacaa ggcccagcgc cttttgacca ttttccagct gggacccaca
     3121 aattgaaatg acggcgagct ccgttatgga gcgtgatgtg gccctgctgt cgggcgatac
     3181 gctcataagc cttgctgagc gcgcgcagga acggcagcgc acgattgtac tggccataga
     3241 ttttttgcgc ttctttgagc gagcggccga gttttttggc ggttgtgcga atgcccatgc
     3301 catagatgaa accaaagttg aatgtcttcg cgtctggccg atcaatacca gccatcagtg
     3361 ccgcaaaaag atgaaaatcg gtatccggat cgtcgtgata gcgctgtagc gcgagctccg
     3421 caccgggcat gttgtgaagc gccgcatagt gcacggcgat gcggaattcc tgctgcgatg
     3481 catccggctt ggcccatatc tcgccttcgt ccggtaagaa caggccgcgg atgatcggtg
     3541 caagctcttc gtcgcgcgcc gtcatcagtt gtaatggcgg gtcggaatac gagaaccgaa
     3601 acgacttcgc accgtggtcc tcggttttga atggatggat ttcagcgtgg atacgaccgt
     3661 tgacgatgtg cccaagaatg aatttgtcga cgaacagatc gccgacacgg tgatactttc
     3721 ccgcctcgcg gattaactgt ggcagccaat gcggatgccc ttccgtccag tccttggcga
     3781 agctcggatt gcctttttcg tttctcgggt acgccactcc ttcgcgatcg cagacctcca
     3841 ctttccaatc gttttggttt agctcgtcca tgctcaccgg catgccaagc ttgtccgata
     3901 gctgattgag cgccgcatcg cgtttgccga gcaagaaatc gcgagctcgc tccgccgccg
     3961 gaatatcgat gcggattccg cgcagctgca tctcgatgat cagcggcact aaattgcatt
     4021 ccagccggta agcggcgcga gtgttttcct ggtcgaggat cggatcgaag agctcgtacg
     4081 tcagcaaagt gttgatggcg tcggtcacag cgtatggcgc gacatatttt gccggtagct
     4141 gccagagata attctgcggg cggaatttct tgcgcttgtt ggcaatcagg tccagcgcga
     4201 gacaaccttc tttcagcaag cgctcgtcct tgccgggtag gccgcaggac ctggctagcg
     4261 catcgagtcc atagccgtgg cggttttcgt tggtcagggt ggcgagcgca cccaattcct
     4321 cgagccgttc gcctgacggc acgcggatgc cggcctcggc acgaaaccag ccccaatcga
     4381 acggtccgtt gtgagtgatg aagctgagct cggaagcaac gtgatccttg agccatgcat
     4441 agacctgctc aggcgggaaa ttctgcgtgt cgggatggcg catcggaaaa tagtgcccgc
     4501 gcacctgccc atccgcacga taggcgacgc tgatgccgca cacatgtccg tcacgccacg
     4561 gccaactcga gccgcggtcg gctgccaatc cgttgtctcg ctcctcgctg tcaattgcga
     4621 tgcggccgac acggcgaaga tctggcagct cgttggggat atgcggatca gacgcagcca
     4681 tggatattgt ctcagtctca ctctgaaatt agccgcgttc tcgccgcagc cggcgcttgt
     4741 cgagcaggtt ctgtagcggt tgcagcgagt accactcgcg cgcgcagagc ggagtgcaga
     4801 agtgcaccgg gaggtctacc ggcgcgtcct ccaggctctt aaacgtgagc tcgcagttcc
     4861 agcagaaggg gccgtcgcca taggactccg cgtccaggta gccgatgatg ccaccgcctg
     4921 gcttatgctg cttgctggcg tcgggcttgg tcatgagcat tgtccccttg cactagtatt
     4981 gaatgctgtc gttgatctct ggccgacccc acgcaattgc acgacgcaag ttgccgtcgt
     5041 agttttcgcc ggcaatgcgg tagccgatga tgcgccaata tttttcacct tctggtgcca
     5101 cgcggatgtg gcttacccag gcgagctcgt cttgccgatt gagggcttca tcgacggtgt
     5161 gtggtaccga caagccgccg ctcaagacgc gccagaactg ttcggcttga ctgcgggcat
     5221 agccgttgtg ttgtaggcaa acccacttgg caaagctctg tgccccacat tgaaatgtca
     5281 cccgcagtga tggggtctct ttgtggtggt agcgatattc gatatcctct acttccaacc
     5341 aatcagatac ggcgcgcggc gggctgagga ttgccacgcg atcggcatag gccgcatgtt
     5401 ttggttcttc tctgcgggag aattggtggc cgcagcttgg acattcggtt gcggccagca
     5461 ggacaatctc atcgcattcc ggacaggtct tggtcggcgc ctcaccgtct ctgctgttct
     5521 tgtgtttgat gtaaacgtta tccaccggac cgaaacggcg aacgttgccg gcgaagtcca
     5581 agacaaggca gtttgctttg ccgtctgcct tgcgcgtttc gcggccgacc tgctggatgt
     5641 agagtccagc gctgcaagtc ggccgcagca ttgcaatgag atcgatgtgc ggaacgttga
     5701 agccatagga gaggacgttg acgctgacca acgcggtcaa tcggccggca cgaaagtctt
     5761 cgatgatgtg atcgcgctct tggtccggcg tctcgcccag caccatttcg gtatggacgc
     5821 cgcgggcacg cagtgcgtcg cgtaccagac cggcatgggt gacgccgaca cagtagacta
     5881 gccaggcccg gcgcttgtcc tggtagctgg caagctcatc gcaggccaat tcgacggcat
     5941 cattgcggat cgccgcggct tctagctgtt cagaaatata ttctccaccg cgcttgccaa
     6001 cgccgctgac atcgattgtc gtgtgggttg tcttcgacga tagcggcgcc agccagtcgt
     6061 catgaatgcc ttcaccgatt gtgtattcga agaccacctt atcaaacaaa tgcccatcgc
     6121 cttcgcacag gtgtccgctg tcgaggcgaa acggcgtcgc ggttagcccg acgacgcgaa
     6181 gatcaggcgc gtgattgcgc agtgcaccta tcgtggtgtg gtacatcccc tgttccccgt
     6241 gcgggatcag atggccttca tcgatgatga ttaggtgccg ctcgccgatt gcatctggat
     6301 tgcgatagat cgagttgatc gtggcaaaga gaatttgcgc atcggtgtcg cggtagccga
     6361 gctcgtcgca gttaatgccg atcggcgctt ctggccatat attctgtagc tcacggatgt
     6421 cctgctcgag cagctcgcga ttgggcgcgg tgaccagtac gcgcatcttc ggatagtcgg
     6481 ttagcaattg cttgatcaga aatgcaatga cgatggactt cccggtaccc gtcgccattg
     6541 caatcaatgg attgcctccg ccgttgcgcc agaagacgaa cagctcgcgg agggcctttt
     6601 cttgatattg gcgcagtatc agcatttgta tgccctcgaa gtgggtaacg atcgccgaca
     6661 ttggctaagt gccggcgacc gttctcatag cggtccatca cgtctgcttc tgattccaag
     6721 gacgatcgcc tttgggcgtt gcagacgctg ttgccatcgc ttcaggcgca ttggccggct
     6781 tcggccctgg gttggcgacc ggagcggcac caggtccacg ccgcttaggc tcgaagtcgt
     6841 acggccgcgc cgcagtcaca cggtttttat ccgagtattc gccattggga tcgcgcctga
     6901 tgttgacgcg gattttgatc ggcttgtgta gaagcacgtc gatgctccgc gtcggcccgg
     6961 tgatgccgca ggcgatgtag atatcggtca taagccgttg gccgatttcg accgcctgtt
     7021 ggcttgcgtt gtggatggtg acgttatgga aaaccttgcg gcccttgtat tggccttcga
     7081 taatctcaaa cgccgtccac aggtagttgc cgttgccgtt ggcagcgtca cgcacctcgg
     7141 cctcgacgat gtgtgcgaga taccaacccg cgggcactgc tacgaggtct tgcgtgccct
     7201 cgtgtgctgc tggatcaaac atctccggca gctggtcgta gtagccatca accatgatta
     7261 acctccttgt tttccgattt cagtttcgat tgcagttttc ggttccgcag tcgtacgcat
     7321 actctccgcc tgcggttgcg gagtcggaaa gaacttgccg agcatcgtct ggtagttgaa
     7381 gttcaggggg acctgaattc gttcgggcat gccgaacctg tttttcgatg tgaatgctgg
     7441 ccgcggatca cagtgcagcc aacgcgtatt gccgccatcg gcttgggcac gggtttttcc
     7501 gaaaccacct tgttcgtttt tgataatgat gtcggttgcc agaaagccga tcaaatcggc
     7561 gttgtcctcg accaatcccc gtgcccgctt acgcagccgc agcgcatacg aagaatacgc
     7621 cgtggtacgc gggtcattga tcatgatgat ctcgctgtgt gcgatcatca cgatgatcat
     7681 gttacgctgg cgtcgcagcc agttgcagcc gcgaagaaag tcgagccaat acttatcgag
     7741 ctcgacgtag cctttgccaa atcctgggct ttcaaccgag gcatagccgc gatcagcaca
     7801 cagtgcagcc tgtacgagcg gctcgggcgc atcgaggcta tcgataacca acgtctgata
     7861 gtcgtgagtt tctttaccca gccaggtcag cgcctcgatg acgctggcga aactgtcgca
     7921 taatccaaag ctcgaaatcg ttaacccgct cggacagccg tcctcagtct ggatgaatac
     7981 tggatttgga aatttcgccg ctgtcgttgt cttgccgatg ccaggagccc catgcatcac
     8041 gacgatcggc aagagcgtgg cggtaacttg aaatggtttc attcggttta ctcctttctg
     8101 taatcttgaa ttccgggata cgggccccat tcctcttccg gccacagcgc ttccgcataa
     8161 ttttcagcac atacgtgaca gcgatattcg atccttgttc gctcgccgaa agttgtcgtt
     8221 gtctttgtcg ttgtccccag gactgtttcg ccgtagggga ttttgctgcc gcaatcgatg
     8281 catctcgtta ttgtcctgat catgccgcac ccctcggtca cgctgttatc tctgcaaccg
     8341 cggccaatct tccttgcgcc gtcgccgctg tgagcggtgc gatcacgatg gtgagcgccg
     8401 gtacggccgc gtatttcttc gtgacgatga gatcgacaac aagactgtcg tcgctgatta
     8461 cgatgttgtt gatgcagtcg agacctacct ttacgaagtt gtcggcgtcg ggccgggttg
     8521 tcggacggac atcgccgcgc agtgccgcgt cttggcgctt gccagaccat gatgttggca
     8581 ctggcagatc gatggtgatt tcagcacgga cgggcgtggt gatcggcgat ttgccgttca
     8641 tcgctagctg cgcggcgagc cgggcatggg cctcgtattt gcgagtggcg gcgggcgtgt
     8701 agacaaagcc gcgtcgcgtt acccgcggtc ggcccttcgc gaccacttcg ccattcacgg
     8761 tgatggtgat tgtctcagtc atggccgcac tgtctccgag acgatggacg cacagaaccc
     8821 gagtcccgaa cgtagcaatg cggtgcacgc gacttcgccc ataccgatca agacgacgcc
     8881 atgaccggga gcggtgccga tagatccatc cgggcggaca aacttggtct tgcccttcgg
     8941 gaacagcagg agctcggcgc gtgggaagac gtactgatga aaccatccgc tcgaggtata
     9001 ggcgcggacg attgcgatgc cgttggcgtg gtcgagaaac ttaaccaacc aggggatgtg
     9061 accgtgtctt ccgccgaatg gcgggttcat aaagaccagt ccgtgccacg gctggcgcag
     9121 tccattgtcc gcgatggtgt agatcttttt cgcaggcgcc cagtgtcctg gaccaggtga
     9181 acagggatcg agatcaaact cgagcccgag cgcgtcgaag tattccggcg gtgtgaacca
     9241 gtctgaagtt tcgccgatgg aaggctcatg catcttcacg gcggcgcgca tcggggtttc
     9301 gttttggctt ttcatgccgc gctctccctc cgtcctccgc ggtcatcatc tctgtgcagc
     9361 catctcaact ccggagaccc tcgatggccg cgggaaaaaa ccaaccacac aaaatcctgc
     9421 gtgccgccgc ctggctcttc gccggcgagg atgacgtgcc ctggcggcat gctcggccgc
     9481 gggctcatca gatagacgcg tgcaagcgga gtgttcacta accaacgggc ggcgtttagc
     9541 cgacgcgcca gccagatcat cgcgactttg cggcgtgcca gtttcagcgc gtgctcggcg
     9601 aaggcgcggc agatatgata cggcccattg gtgatgacgt tatcgacgcg gcattcgctg
     9661 cggagaaagt cctcagcgtc tacgaattgc agataaccgc ggtcgactag atctgtggca
     9721 attacgcgat agccggcggc gcgggcggcg tcggtgatgc gtccgattcc gcaagctgga
     9781 tccttgataa ccccatcgaa ccgctcgacc tcgaacaatc gacgcgagca ccattccggc
     9841 tcgatataga atccgtgcgg gtccttggtc caaacgtgcg ccaataacgg acggcggtcg
     9901 gtacgcttca acatgacact gactcttttt actcagcttc acttcactga gagagttgtg
     9961 cggcgagcgg tagccttgaa cgagcaacgc atttcgcttt tttcagagac gcgactcgca
    10021 ggctaccttt cgattcccgg cgtggccgct ggccggttga agatctcgct cgcgccctgg
    10081 aagacggtta ctcgactcct tcacgctgtc gcgtaattcg ttcgtgttgc ttgagcatcg
    10141 ccagcgccgc cacttagttg cgacggcgct tatctgcacc ctcatgaaaa ttcgcagccc
    10201 tcgagctctg acgatgcgct ctgaagctcg ctgcaaaagt cattcgcttc ctgatggcgt
    10261 ggatcgctct catcaatggc gtcgagcgcc tccgcgcacg cgctaatgat gccaagcgcg
    10321 gcgtcccgct gagcaccgcg agatcgcaac cgacgaggtt taggcagctt gactttgatc
    10381 ccgccgagcg cggccgggac gtcgggcata ctcaagctct caagcacgcc cgccgtctcg
    10441 tcgagggctt gaatccgcgc tgactgatcg aggcccgctg gtgcgttatc gacctgcccg
    10501 cgacagccgc aagcgaggtc ctctatttcg tcgaacgcat tggccacggc atcctgcgga
    10561 gtgacgtccc cgccggattc ggacgtcccc gccgccgccg gcttgcgttg caggaactcc
    10621 gggatcggcc cggggtcgcc agcagtcacg gtgtcggttg ccgcgatctt cgggctcgcc
    10681 gccgccgcct tctgcgccac ctcgagcttc acgcgcagcg caacgttctc cgccttgagc
    10741 tcgctgtttt cgctttcgag cccctcgatc ttgagctcaa gcaggcgaca cttattctcc
    10801 agttcttcgt tgcgagcctt cagccacccg acctcgccgg tgctggcgac accattgtcg
    10861 gcgctcgtcg ccccgatgtc gttacgcgca ggcgtaggcg caggaggggg cgggggcggc
    10921 gtggtcggct gcttgtggtg cttggtgccc ttgtgccgct tgacgacgcg cttgacctcg
    10981 gcgacaggca gcgactcgcc agcctcggcg cgctcgatga tctcggtctt cgccggttct
    11041 ggcgtcgatg gtgcggtcaa gagatagagg ccggacagcg gcaagctcaa attgccaaac
    11101 tttggcaatt tggcccgcgc ttcgtagacg tggataaagt tgtcagcggt ctggcgcgac
    11161 cactggaatt cccgttcaag ccaggggagc cattgaccat ggtcgagatg tcggtcgcgg
    11221 cattcaacca accggcggcc gatctccacg atgttctcgg cggtttgctt gccgagcgcg
    11281 cggatcgcga cggcgtgttc ggccaggaac gggttgtcga gcggggtgcg cttggtcgcg
    11341 gcgaccccct ggggcgcttc accgttgatt ttctgtaaag ttccgctatt taacccgtcg
    11401 tggtccattg gagacctcac tttccggggc ggcgcttcgg acttagaacc tctcggtagc
    11461 gccgaccccc ctctccttct tctcaatcac atcacgacga cctcagaccg cgttcagcac
    11521 cgctcgcgat atgggctcgg cgcggcacgc tgcgtttgcg cggcgaagga ggcttgttcc
    11581 gtagcgtcac agagattgcg agcggtgcgg ttgagcgcgg cggtttggct gcgcgtagga
    11641 gcaaccgctt gacctgccga tcccatttat tgcgcggcac cagcatcagc ttgccgcccg
    11701 caccggcgcg ctcgaccggg atcaggccag cctcggcggc gtcatacgag cccgtgcgcc
    11761 ccaggccgat ctgcgcgcca gcctgtggga ctgtgtaata ggagcggagc cgcaataacc
    11821 ggctcatcgt gacgagcggc gccatcggtc tctccgccgg acgagttcgg ataaatctat
    11881 cccgttttca gaatactgta aacccgtttt aggaatattc ctgttttaga acgtagacag
    11941 ccggcttttg ggggattagt atcccgtaca ctttcgtccg aatttcatcc ggaaaggaca
    12001 ctgttcatgg tgcgcaagca ggtcagcgac ccctccgagc acgagcttga attcaagatc
    12061 aaattgcccg ccgatatcgg cgaacgcatt cgcgccaagg cgcaacgcga aggccgaccg
    12121 ctcaatcgtg taatcaccga tgagctccgc gagtatccgg tgttaaagga aacaggcacg
    12181 ctcgccgcgg ccgttgacgc actcgctgag caaaatgcca cactcgctga gcaaacccgt
    12241 gagatgaaaa tttctctcgc gcggcacggt gcccgcatcg attggctcga tctgaccgac
    12301 gaattagtga acgctataga tttagtgaat gaggcactcg agacagatag cccctctctg
    12361 ccagccgcca tggacgcttt gcgtgcagga cttctaacgg cgcgcacctt gggtccggaa
    12421 gtgaaacgca aactcaaaaa accgtaatga ctctccagga gtctcgccgt tggtcacggg
    12481 cgacagtatt cggttctcag tagtgcctcg cgacgtacca gcagcaaagg cggcacggcg
    12541 gctgcatctc acgctgaacg agtttgagga gatgaaagac gaactgtttc gccgcggttt
    12601 cccgcgcccc gatccgacca ccggcaattt cgacctggtc gagatcgacc gttggatgga
    12661 cggccgccac caccactgtg attcaaacaa gccgcgcgac gcgcgcgagc tcctgccacc
    12721 gggattgcgg cgatgaccac cgacaagatc cgctacctga gattccttaa cgggcaatgg
    12781 cgctggcgtc cggaagccta catgcgggcc tacgggttca agctgaccac gctcggtccg
    12841 gagaaaactg cgagcgtgat cgcccgtgct gccgcgctca acgaggaatg ggaccgagtt
    12901 aaacgtgggg ctgcactcgc cgcgcaatca atcgcgatca aatatccgcc cgggaccatc
    12961 ggcgacggat atacgcgcgc catggaaagt cgcgccgccg atcgggctga agcgggcaag
    13021 ccgcagacca aggaccagga aaagcgcgac gactggaagc gcgcctggaa acatctcgag
    13081 cgctgggcgc tttatgatcc gaccacaatc cagcccgagc attttctcgc catcgatccg
    13141 gtcaccggac agacggttgg gctgattgcc gatctcaaca gggtctcggc gggcgagcgg
    13201 caccgcacca tcaaagtctg gcgcgccctg tggaagcgta tggcgcggct cggctattgc
    13261 gcactcgagg cggacccgag caaagtgcgg cccaattcag caccacagcc gcgccaggcg
    13321 gtgtggatgc atcgcgaggt gatgaagctc gtccagggcg catggcggtc gggcaaacgc
    13381 gggctcgcct gcttcatcgc catcacgtgg gacacgatgc tctccccggt cgacgcgcgt
    13441 gccctcacct tggcgcagat gcgcgagcgt tcgttcttta cggttgcgcg ggccaagacc
    13501 ggccgcgccg cgttggcgac gctctcgcct tattcgcagg cgctcctggc ggcctatctc
    13561 ggtccagtgc agccgctcaa cgatgcgccg atcttccgca ccgccggcag cgaaccgggg
    13621 ccgaaggggg gccgccggtg gacgccggtg ccttacacca aggacaagct ggagaaggac
    13681 ttcgccgagg tccgcgccct ggtgtttggt cccgcagaac gccggcagat gtcagatctg
    13741 cgccgctcag gccatgtcga gggtgatgct ggtggcgcca cgccggtcga cgcatcagcc
    13801 aaaatggcga atacgatcgc cgtctcgaat cgactgcaca agacttacaa cccggtcaac
    13861 atcgcctcgg tacagcgctt cgacgaggcg cgcaaagtcg ggcgcggcaa gttgaaaggt
    13921 tgaacatcgc gacttagccg cggcccgctc gcaagacggt taagccggaa caaacgaaca
    13981 caaaaactgt cacaaccgtc ggccaaaaac tgtcacacgc aggtgcagca ttcagctaac
    14041 tcattgattg caatggcggg agcgacgggg ctcgaacccg cgaccttcgg cgtgacaggc
    14101 cgacgctcta accaactgag ctacgccccc gcgggcgtgg tgcggagtta aataccgacc
    14161 accggcgagt caaggcagcg cccgaaaagc cc
//
LOCUS       DATLJT010000724         1536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3031522,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000724 DATLJT010000000
VERSION     DATLJT010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3031522"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VM910_16565"
     CDS             complement(<1..300)
                     /locus_tag="VM910_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVI64177.1"
                     /translation="MRENAQIIRRRDLLSLIGAMAGSSAMYHAMTSLGFAAESRYAGP
                     IRLEGDPKGASVLILGAGLAGMTAALELRNAGYKVKVLEFNARPGGRNWTLRGGDR"
     gene            524..1393
                     /locus_tag="VM910_16570"
     CDS             524..1393
                     /locus_tag="VM910_16570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HVI64178.1"
                     /translation="MTDSSILSSLSSALADVVARARPSIVSVQSRRLRASGFVWKGGL
                     VVTADEALAEEGEISVRLADGTMRPASIAGRDHTTDVALLRVDTSGLAPLKLSTRLPQ
                     LGSLAVVVAAENGLPAAALGAVSLVGERWRSLRGGEIDARVELDVRLSHGRQGGLALD
                     PSGGAFGMAVLGPRRVLVIPSATIERVAGKLETHGRVARGYLGLGLQPVKLDDGLGAM
                     VMSVDKAGPSASAGIRQGDVIVSWNNEKLSGVRALLRALGPESVGSVVDVAVQRAGEP
                     KTFKLTIGERPEA"
     gene            1390..>1536
                     /locus_tag="VM910_16575"
     CDS             1390..>1536
                     /locus_tag="VM910_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI64179.1"
                     /translation="MSEETSPTIAIRIEIDDPALADRLASLLGGIAGLRLAAPGEAAD
                     VAIVS"
BASE COUNT          247 a          506 c          512 g          271 t
ORIGIN      
        1 gcggtcgcca ccccgcaagg tccagttgcg gccgccgggg cgcgcgttga actcgagcac
       61 cttgaccttg tagccggcgt ttcgcagctc gagtgccgcc gtcatgccgg ccaatcccgc
      121 gcccaggatc aggacggacg cacctttggg atcgccttcg agcctgatcg ggcctgcgta
      181 acgggattcc gccgcaaaac cgaggctggt catggcgtga tacatcgcgc tgctgccggc
      241 catcgctcca atcagcgaga gcagatcgcg ccttctgatg atttgagcgt tttcgcgcat
      301 gccgatcgaa tgtcgcaaac cggttgcgtg tgcaatccag gccccgagtt ctcgaaggga
      361 accgggcggc tgccgccctg tcaagaaata cgcatccgct gcacacgctt tgttgaacgg
      421 tgagagctcc cgggtgcagc gacacctgcg ccccggtcat ccgggcagtt acgcaaacgc
      481 atgcgaagtt ccagactagc gtcaagcctg acgggagtta cccatgaccg attcctccat
      541 tctgtcttcc ttgtcctccg cgctcgccga tgtcgtggcg agggcgcgac cttccatcgt
      601 ctccgtgcaa tcgcgtcgcc tgcgcgcttc cggcttcgtc tggaaaggcg ggcttgtcgt
      661 aaccgcggac gaggccctcg ccgaggaagg cgaaatctct gtcaggcttg ccgatggaac
      721 gatgcgcccg gccagcatcg cgggccgcga tcacacgacc gacgttgcgt tgctccgggt
      781 cgacacaagc ggacttgcgc cattaaaatt gtcgacgcgc ctgccacaac tcggatcgct
      841 cgccgtcgtg gtcgccgcgg agaacgggtt gcctgccgct gcgcttggag ccgtgtcgct
      901 ggtcggcgag cgctggcgca gcctgcgcgg cggagagatc gacgcaagag tcgaactcga
      961 cgttcgcctg tcgcacggcc gccagggtgg gctggcgctg gacccctccg gcggagcgtt
     1021 cggcatggcg gtattgggtc cgcgccgcgt gctcgtcatt ccttccgcga cgatcgagcg
     1081 cgtcgccggc aagctcgaaa cccacggccg cgtcgcgcgc ggctatctcg gactcggcct
     1141 gcagccggtc aagcttgacg acggcctagg cgccatggtg atgagcgtgg acaaggccgg
     1201 cccgtcggcg agcgccggca tcaggcaggg tgacgtgatc gtgtcatgga acaatgaaaa
     1261 gctgtccggc gtgcgcgcgc tgctacgggc gcttggcccc gaaagcgtcg gttccgtcgt
     1321 ggacgtggcg gtccagcgtg ccggcgaacc caagaccttc aagcttacga tcggcgaaag
     1381 gccggaggcg tgagtgaaga gacttcgccg acgatcgcga tcaggatcga gatcgacgat
     1441 cccgccctcg ccgaccggct ggcgtcgctc ttgggcggca tcgccggatt gcgcctggcc
     1501 gcacccggcg aggctgccga tgtcgcaatc gtgtcg
//
LOCUS       DATLJT010000725         6953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_275091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000725 DATLJT010000000
VERSION     DATLJT010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VM910_16580"
     CDS             <1..1003
                     /locus_tag="VM910_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HVI64180.1"
                     /translation="NIARIQKACEAAGVANRPHIKTHKSPLLAKMQIAAGASGITCQK
                     LGEAEVMAEAGIDDILISYNLIGDEKMARLGALQRKANMTVAADNSTVIAGLTKAAET
                     SGRPLPVVVECDTGRKRAGVETPAEAVRLAREIAASKGLVFAGFMLYPTETGWAEAQR
                     FYDEALAGVRAQSQEVKMVSTGGTPNMKNVGKLKGATEHRPGTYIYNDRMQVAAGVAT
                     WDDCALNIYSTVVSRAAPERGILDAGSKTLTSDPGGGLDGFGLILEHPEARIARFAGE
                     HGFLDLSRSNTRPNVGDVVRIVPNHVCVVVNMMDEVVMVRGDEILGVLPVAARGKLR"
     gene            complement(1063..2316)
                     /locus_tag="VM910_16585"
     CDS             complement(1063..2316)
                     /locus_tag="VM910_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HVI64181.1"
                     /translation="MNAAVIGAGPAGLMAAEVLAQGGVRVTVFDAMPSAGRKFLMAGR
                     GGLNLTHSEPLPLFLSRYREAASRLAPVIAAFPPDSLREWSEALGQPTLVGSSDRVFP
                     KAFKASPLLRAWLRRLGSSGVQFNFRHRWTGWSDQGDLRFQTPHGQHGVWARATVLAL
                     GGASWPRLGSDGAWASVLAARGVAISPLRPANCGFAVTWSNLFRDRFEGEPLKGVEFR
                     LGSERARGEAVITKAGIEGGAIYALSAELRETIAKSGEAVLRIALRPDLTETDLIARL
                     SLPRGKQSLSNFLRKAARLPPVAIGLLQEAAIASGAPLSSLSPAQLARLINDLPLTLV
                     GPAPIARAISSAGGIRLDELDPDFMLRRLPGVFAAGEMLDWEAPTGGYLLQACFATGV
                     AAGRGALKWLNPHYEERSNAARLEP"
     gene            complement(2320..3237)
                     /locus_tag="VM910_16590"
     CDS             complement(2320..3237)
                     /locus_tag="VM910_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI64182.1"
                     /translation="MAIRFDGRVAVVTGAGNGLGRAHALGLASRGAKVVVNDFGGARD
                     GTGGSMTPAQNVVEEIRKAGGTAMADGADVSNFEQVKAMVERATKEWGSVDLLCANAG
                     ILRDKSFAKMELADFAKVIQVHLVGAYYCCKAVWEGMRERNYGRIVLTTSASGLYGNF
                     GQANYGAAKAGMVGLMNVLAEEGRKNNIRVNTISPTAATRMTEELLPPEPLKLMRPEA
                     ITPAVEYLLSEDAPTRTILGAGAGSFALIRIMETEGINLPPSEWTPDAIAAHFQDISD
                     MSTAKALENAFQQTQKYVTQAAARAGVKL"
     gene            complement(3395..3874)
                     /locus_tag="VM910_16595"
     CDS             complement(3395..3874)
                     /locus_tag="VM910_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVI64183.1"
                     /translation="MNEVWKKPPVSLSAYQAMVGKEVGVSSWHLVDQTRINGFADVIE
                     DHQFIHIDPERAKRETPFGSTVAHGFLTMSLLSIMSYEVMPVIEGTTMGVNYGFDKLR
                     FISPVRAGSRVRGRFVLAEAKLRKEKELQSRTNVTIEIEGEEKPALVADWLGLIYFT"
     gene            complement(3878..5614)
                     /locus_tag="VM910_16600"
     CDS             complement(3878..5614)
                     /locus_tag="VM910_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI64184.1"
                     /translation="MTSFREARAFLLQNRADYDAAVKGFRWPDPVPFNWALDWFDADL
                     ARDPESRDRAALWIVDAETNRDTKLSFAGLSRRSNQVANFLRAQGLRRGDHLLLLLGN
                     VVPLWETMLAAMKLGVVVIPATTLLTSGELQDRLDRGRARVVVATQDQVGKFASLRGE
                     DITRIVVGASSKLEGWIPFEQAAEFPEDFVPDGPTQANDPMLLYFTSGTTAKPKLVRH
                     SQRSYPVGHLSTMYWLGLKPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRF
                     EAKSLLATIARCGVTTLCAPPTVWRLFIQERLSDFKVSLREVCGAGEPLNPEVIDQVR
                     EAWGLTIRDGYGQTETAAIVGNSPGQKIKVGSMGRPLPGYRIQITDPDGQVTKEGEVT
                     LKLGADRPAGLMQGYQGEDGKLSGVDGELYRSGDVVFADDEGYLTFVGRSDDVFKSSD
                     YRISPFELESVLLEHDAVAEAAVVPCPDPIRLSIPKAYVLLVAGVERSPETALSIFKH
                     LHGRLGPFKRIRRIELVTELPKTISGKIRRVQLRRLEHDDNREDPLRGSEFREEDFPE
                     LQRVRTARATES"
     gene            complement(5773..6663)
                     /gene="mmsB"
                     /locus_tag="VM910_16605"
     CDS             complement(5773..6663)
                     /gene="mmsB"
                     /locus_tag="VM910_16605"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HVI64185.1"
                     /translation="MATIAFIGLGNMGGPMAANLVKAGYKVVAFDLVEASRNQAKSDG
                     AAVAESAAAAVKGADTVVTMLPAGKHVLTVWNEVVPIMTKGTLIIDCSTIDVESSKAA
                     HVLAAKHGVGSVDAPVSGGTGGAKAATLTFMCGGDEKSFAAAKPVLEKMGKKIVHCGG
                     AGAGQGVKICNNMVLAISMIGVSEAFSLAEKLGLSHQAVFDVVSTSSGQCWSLTSYCP
                     VPGPVPTSPANNDYKPGFASALMVKDLTLAQDAAKAAGAVTPLGKHAQEIYKAFDAAG
                     HGGVDFSGIIQHVRELPVRS"
     gene            complement(6695..>6953)
                     /locus_tag="VM910_16610"
     CDS             complement(6695..>6953)
                     /locus_tag="VM910_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI64186.1"
                     /translation="RGMVVALKLLRLARQSSSLEECLVREYRAALEVFRSDDFREGVR
                     AAVIDKDRNPKWSPPRIEDVTPDMIAPYFANIGADELVFDQ"
BASE COUNT         1331 a         2255 c         2110 g         1257 t
ORIGIN      
        1 caacatcgcg cggattcaaa aggcgtgcga ggcagccggc gtcgccaatc ggccgcacat
       61 caagacccac aagagcccgc tgctcgcaaa gatgcagatc gcagccgggg cgagcggcat
      121 cacctgccag aaacttggcg aagccgaggt gatggcagag gccggtatcg acgacatcct
      181 gatctcctac aacctcatcg gcgatgagaa gatggcacgg ctcggcgcgc tgcagcgcaa
      241 ggccaacatg accgtagccg ccgacaattc caccgtgatc gccggactga cgaaggcagc
      301 ggagacgtcc ggccggccgc tcccggttgt ggtcgaatgc gacaccgggc gcaagcgcgc
      361 gggggtcgag accccggcgg aagcggtgcg gctcgcgcgc gagatcgctg cctccaaggg
      421 attggtgttc gccggtttca tgctttatcc gaccgaaacg ggttgggcgg aagcgcagag
      481 attttacgac gaagccctgg ccggcgtacg agcgcagagc caggaggtaa aaatggtctc
      541 gaccggcggc acgccgaaca tgaagaatgt cggcaaactc aaaggcgcaa ccgaacaccg
      601 accgggcacc tatatctaca acgaccgcat gcaggttgcg gccggggttg cgacctggga
      661 cgattgtgcg ctgaacattt attcgacggt ggtgagtcgc gccgcgcccg agcgcggtat
      721 tctcgatgcg ggctccaaga ccctgacatc tgatccgggc ggcgggctcg atggttttgg
      781 attgatcctc gagcaccccg aggccaggat cgcgcgcttt gccggagagc acggctttct
      841 cgatttgtcc cgcagcaaca cccgacccaa tgtcggtgac gtcgtgcgga tcgtgcccaa
      901 tcatgtctgt gtcgtcgtca atatgatgga cgaagtcgtg atggtgcgcg gagacgaaat
      961 tcttggcgtg ctgccggttg cggcgcgggg gaaactgcgc tgattttcct cacgtcgagg
     1021 cgcacgccct tgcgcgtgtc gcggagaaag agaccaaagg cctcatggtt cgagacgcgc
     1081 ggcgttgctg cgctcctcat aatgagggtt tagccacttc agtgctcccc gccccgccgc
     1141 cacgccagtc gcaaagcagg cctgcaataa atagccgccg gtcggcgctt cccaatcgag
     1201 catttcgccc gccgcaaaca cgccgggcag acgacgaagc atgaagtcgg gatcgagttc
     1261 gtcgagcctg atcccgccgg cactcgaaat cgcgcgcgcg atcggcgcgg ggccgacgag
     1321 cgtgagggga aggtcgttga tcagccgcgc gagctgcgct ggcgacagcg aggatagcgg
     1381 agcacctgag gcaatcgccg cttcctgcaa caggccgatt gcaaccggcg gcagtcgcgc
     1441 cgcctttcgc aaaaaattcg aaagcgattg cttgccgcgc ggcaaggaga gtcgtgcgat
     1501 cagatcggtc tcagtgagat cgggccgcaa ggcaatgcgc aagacagctt cgcccgattt
     1561 tgcaatcgtt tcccgcagct cagcggacag cgcatagatc gcgccgccct cgatgccggc
     1621 tttggtgatg accgcttcgc cgcgagctct ctccgaaccg agtctgaatt cgacgccctt
     1681 caggggctcg ccttcgaagc ggtcgcgaaa gaggttggac catgtaacgg caaatccgca
     1741 attcgccggc cgaagcggcg atattgccac gcccctcgcc gccagcacgc ttgcccacgc
     1801 gccatcggag ccaagccgcg gccagctcgc gccgcccagc gccaacacgg tcgcgcgtgc
     1861 ccagaccccg tgttggccgt gcggagtctg aaatcgcaga tcgccctggt cgctccagcc
     1921 ggtccagcga tgacgaaagt taaattgcac cccgctcgaa ccgagcctgc gcaaccaggc
     1981 gcgcagcagg ggcgaggcct tgaaagcctt cgggaacacg cggtcgctag agccaacaag
     2041 ggtcggctgc cccagcgctt cgctccattc gcgcaaactg tccggcggga aagccgcgat
     2101 tacgggcgcg agccgcgacg ccgcctcgcg atagcgcgac agaaagaggg gcagcggctc
     2161 gctatgcgtc agattgagcc cgccgcgacc ggccatcaga aacttgcggc ccgccgatgg
     2221 catggcatca aagacggtaa cgcggacgcc gccctgcgcc agcacctctg ccgccatcag
     2281 cccggcgggg ccggcgccga tgacggcggc gttcatggat caaagcttca cgccggctcg
     2341 cgccgcggcc tgggtgacgt atttctgggt ttgctggaac gcattctcca acgccttcgc
     2401 cgtggacatg tcgctgatat cctggaaatg ggcggcgatt gcgtcaggcg tccactcgga
     2461 cggaggcagg ttgatgcctt cggtctccat aatcctgatc agcgcaaacg atcccgcccc
     2521 cgcgcccagg atggtgcggg tcggcgcgtc ctcactcaag agatactcca ccgcgggggt
     2581 gatcgcctcc ggtcgcatca gcttgagcgg ctcgggcggc agcaactctt cggtcatgcg
     2641 cgttgccgca gtcggcgaga tggtgttgac gcggatattg ttcttgcgac cctcttccgc
     2701 cagcacattc atcagcccga ccatgccggc tttcgccgcg ccgtaattgg cctggccgaa
     2761 attgccatag aggccggagg ccgaggtcgt cagcacgatg cgaccgtagt tgcgctcgcg
     2821 catgccctcc cataccgctt tgcagcaata gtaggcgccg acgagatgca cctggatgac
     2881 cttggcaaag tccgcaagct ccatcttggc gaaggatttg tcgcgcagga tgccggcgtt
     2941 ggcgcagagc agatcgacgc tgccccattc cttcgtcgcg cgctccacca tcgccttgac
     3001 ctgctcgaaa ttggagacgt cggcgccgtc agccatcgca gtcccacccg ccttgcggat
     3061 ctcctcgacc acgttttgcg ctggtgtcat cgatccgccg gtgccgtcgc gtgcgccccc
     3121 gaaatcgttg accacgactt ttgcgccacg gctcgccagc cccagcgcat gcgcgcgacc
     3181 gagaccattg cccgcgccag tgacgacggc gacgcgtccg tcaaatctga tagccatgag
     3241 tgcaatcccg gatctggttc tctcatcatg cccggacttg tccccgggca tccatccaac
     3301 aaaaatccac ttgtttttga tcagtgatgg attgccgggt caagtagttg aagacgcgct
     3361 tcgcgctttt accaggtaat gacagcggag attgttacgt aaagtagatc aggccgagcc
     3421 aatcggcgac gagtgcgggt ttttcctcgc cctcgatctc gatggtaaca ttggtgcgcg
     3481 actgtaactc cttctccttg cgcaatttcg cttccgccag cacaaagcgg cctcggacgc
     3541 gcgagcccgc gcgcaccggc gaaatgaagc gaagcttgtc gaacccgtag ttcacgccca
     3601 tcgttgtgcc ctcgatcacg ggcatcacct cgtaggacat gatggagagg agcgacatgg
     3661 tcagaaagcc atgcgccacc gtcgagccga acggcgtttc ccgcttcgcg cgctcggggt
     3721 cgatgtggat gaactggtga tcctcgatca cgtcggcgaa gccattgatg cgcgtctgat
     3781 cgacgagatg ccacgaggag acgccgacct ccttgcccac catggcctgg taggcggaaa
     3841 gcgacaccgg cggcttcttc cagacttcgt tcatccatca actctccgtc gcccgcgcag
     3901 tccgtacgcg ctgcagctcc ggaaagtctt cctcgcgaaa ctcgctgccg cgcagcggat
     3961 cctcgcgatt gtcgtcgtgc tccagccggc gcaactggac gcggcggatc tttccggaaa
     4021 tcgtcttcgg cagctccgtt acgagctcga tgcgccggat gcgcttgaac ggcccaaggc
     4081 gcccgtgcag atgcttgaaa atcgagagcg ccgtctccgg cgaacgctcg acgccggcaa
     4141 ccagcaacac gtaggccttg gggatcgaaa gccgtatcgg atcggggcac ggcaccacgg
     4201 ccgcttcggc gaccgcgtca tgctcgagca gcacgctttc cagctcgaac gggctgatgc
     4261 ggtagtcgga cgatttgaac acgtcgtcgg agcgcccgac aaaggtcaga taaccctcgt
     4321 catcggcaaa gacgacgtcg ccgctgcgat agagctcgcc atcgacgccg ctgagcttgc
     4381 cgtcctcgcc ctgatagccc tgcatcaggc ccgccggacg gtcggcgccg agcttgagcg
     4441 tgacctcgcc ttccttggtt acctgaccat ccggatcggt gatctgtatg cgatagcccg
     4501 gcaagggacg gcccatggag ccgaccttga tcttctgtcc cggcgaattg ccgacgattg
     4561 ccgctgtttc ggtctggccg tagccgtcgc ggatggttag gccccaggct tcgcggacct
     4621 ggtcgatcac ctcgggattg agcggctcgc cggcgccgca gacttcgcgc aggctgacct
     4681 tgaagtccga cagccgctcc tggatgaaca gccgccatac cgtcggcggc gcgcacagcg
     4741 tggtaacgcc gcaacgcgcg atagtggcaa gcaggctttt ggcctcgaag cgcggctggt
     4801 tcaccacgaa aacggtcgcc cccgcgttcc atggcgcgaa gaaacagctc caggcgtgct
     4861 tggcccagcc gggagaagag atgttcaggt gcacgtcgcc cggcttaagg ccgagccagt
     4921 acatggtcga gagatgcccg acgggatagc tacgctggct gtgccgcacc agctttggtt
     4981 ttgcggtcgt gcctgaggtg aaatagagca gcatcggatc gttggcctgt gttgggccgt
     5041 cgggcacgaa atcttccggg aattccgccg cttgttcgaa tggaatccag ccctcgagct
     5101 tcgaggaggc gccgacgaca atacgggtga tgtcctcacc acgcagactt gcaaattttc
     5161 cgacctgatc ctgcgtcgcc acaacgaccc tcgcccggcc gcggtcgagc cggtcctgca
     5221 attcgcccga ggtcaatagc gtggtcgcgg ggatgacgac gacgccgagc ttcatcgccg
     5281 ccagcatggt ttcccagagt ggcacgacat tgccgagcaa cagcaagaga tgatcgccac
     5341 gcctcaatcc ctgcgcacgc aggaaattgg caacctggtt tgagcggcgc gacaagccgg
     5401 caaaggagag cttggtgtcc cggtttgtct cggcatcgac gatccacagt gccgcgcgat
     5461 cccggctttc aggatcgcgc gccaggtccg cgtcgaacca atcgagcgcc caattgaacg
     5521 gcactggatc cggccagcga aatcccttca cggccgcatc gtaatcggcg cgattctgaa
     5581 gcagaaaggc gcgcgcttcc ctaaaactgg tcatcggatc ttaccggctg gttgattagg
     5641 tctgacattc tctaatcttc tgcgtgctag tcgcagagcg aatgtcagat ccacccacca
     5701 gcaaacggag gcggctagtg gtcttttgat cctgacattc gcaggaacgc ccgtcccgac
     5761 tgaatgcaca tgtcaggatc ggactggtag ttcgcgaacg tgctggataa ttcccgaaaa
     5821 gtccactccc ccgtggcccg ctgcatcgaa cgctttatag atttcctgcg cgtgcttgcc
     5881 gagcggggtg acggcgccag ccgccttggc cgcatcctgc gccagcgtga ggtccttcac
     5941 catcagcgca gaggcaaagc caggcttgta gtcgttgttc gcgggcgagg tcggcacagg
     6001 acccggcacc gggcaatagg aggtcagcga ccagcactgt cccgacgagg tggacaccac
     6061 gtcgaacacc gcctgatgcg acaaccccaa cttttccgcg agcgaaaagg cttcgctgac
     6121 accgatcatg gaaattgcca ggaccatgtt attgcagatc ttgacgccct gacccgcgcc
     6181 ggcgccgccg caatgcacga tcttcttgcc catcttttcc agcactggct tcgccgcagc
     6241 aaacgacttt tcgtcgccac cgcacatgaa ggtgagtgtc gcagcctttg cgccgccggt
     6301 gccgcccgac accggcgcgt cgaccgagcc gacgccgtgc ttggccgcga gcacatgtgc
     6361 cgctttcgag ctttccacat cgatggtcga gcaatcaatg atcagcgtac ccttggtcat
     6421 gatgggaacg acctcgttcc acaccgtcag cacgtgcttg ccggcgggaa gcatggtaac
     6481 gaccgtgtcg gcacccttca ccgcggccgc cgcgctttcc gcaacggcag cgccgtcgct
     6541 cttggcctga ttgcgcgaag cctccaccag atcgaacgcg accaccttgt agccggcctt
     6601 gaccagattg gccgccatag ggccgcccat gttgccgagg ccaatgaatg cgatcgttgc
     6661 catcaaaatc tcctccggta agccgtttct atttttattg atcgaagacc agttcgtcgg
     6721 cgccaatatt cgcaaaatag ggcgcgatca tatcaggcgt gacatcctcg atccgcggcg
     6781 gggaccattt cggattgcgg tccttgtcga tgacggccgc gcgcacgccc tcgcggaaat
     6841 cgtcgctgcg aaacacttcc aacgccgcac gatattcccg caccagacat tcttccagcg
     6901 aagaggattg ccgcgccagc cgcaacaact tgagcgccac caccatccct ctc
//
LOCUS       DATLJT010000726         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2342137,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000726 DATLJT010000000
VERSION     DATLJT010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342137"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VM910_16615"
     CDS             <1..1615
                     /locus_tag="VM910_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI64187.1"
                     /translation="AGASNADAAFVKHCAEHPIRPGRGSVAGRTALEQKTVHLPDCLA
                     DPEYTLLDFQEVGGHRSMLGVPLLRDGVTIAVIVLLRNAVRPFTDKQIDLVTTFANQA
                     LIAIENVRLLEAEQQRTRELSASLQQQTATADVLKVISRSAFDLKSVLQTLVESAGRL
                     CGADYATITRQKDGVLFFAEAYGYSSEFIEYVKAMPVERGRGTATGRALLERRVIHIA
                     DVLADANYTWAEAQRLGGYRTVLAVPMLREGIPMGVLTLTRSEVRPFTEKQIELVSTF
                     ADQAAIAIENVRLFDEIQDKSRQLAEASERKSQFLASMSHELRTPLNAIIGLTEMMVT
                     NAARFGTEKALEPLRRVNAAGTHLLSLINEVLDLSKIEAGKLELNPERVNLARLIDEV
                     VGTAGQLAEKNQNRLTVEAQENVGSLTADSLRLKQILLNLLSNACKFTKEGEVALRVR
                     KVADGRGWVELAVADSGIGMTAEQQAKLFQDFTQADSLTARRYGGTGLGLALSRKLAR
                     MMGGDVTVTSEPGKGSVFTVRLPVGTETH"
     gene            1960..2316
                     /locus_tag="VM910_16620"
     CDS             1960..2316
                     /locus_tag="VM910_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64188.1"
                     /translation="MSENGTETPEPVVKFEPKARPRSHDTPLDEAGQAIVARIQRAAE
                     LANENCDRAMKLAHKLAMQLRAAEERINQLEAEVELFRDRAARAEGWLQTIQKEIEEK
                     LITPRLASGTEQRSLP"
BASE COUNT          515 a          793 c          841 g          461 t
ORIGIN      
        1 ggcgggtgcg agcaatgcgg atgccgcatt tgtgaagcac tgcgcggaac acccgattcg
       61 tccaggacgc ggttcagtcg ccggacggac ggcgctggag cagaaaaccg ttcacttgcc
      121 ggattgcctg gccgatcctg agtacaccct tcttgacttt caggaggtcg gtggccatcg
      181 cagtatgctc ggggttccgc tgctgcgcga cggcgtgacg atcgccgtca tcgtactcct
      241 gcgcaatgcc gtgaggccgt tcacggacaa gcagatcgac ctggtgacga ccttcgccaa
      301 ccaagcgttg atcgcgatcg agaacgtgcg cctgctcgag gccgaacagc agcgcacgcg
      361 cgagctcagc gcgtcgctgc agcagcaaac tgcgaccgcc gatgtcctca aggtcatcag
      421 ccgctcggcc ttcgatctca agtcggtact gcaaacgctc gttgaatcag ctggccgcct
      481 ttgcggcgcc gactacgcca cgataactcg gcaaaaggac ggggtgctct tcttcgcgga
      541 ggcttacggt tactcgtccg agttcatcga atatgtgaag gctatgccgg tcgagcgggg
      601 gcgcgggacg gccaccggac gggccttgct ggaacgccgg gtgatccata tcgccgatgt
      661 cttggccgac gcgaactaca cctgggcaga ggcccagagg ttgggcggct accgcacggt
      721 cctcgccgtg cccatgttgc gcgaaggcat cccgatgggc gttctgacat tgacacgctc
      781 cgaggtgcgg cccttcaccg agaagcagat cgagctcgta tcgactttcg ctgaccaggc
      841 agcgatcgcg atcgagaacg tgcggttgtt tgacgagatc caggacaaga gccggcagct
      901 ggcggaggca agcgagcgca agtcgcagtt cctcgccagc atgagccacg agctgcgcac
      961 gccgctcaac gccatcatcg ggctgacgga gatgatggtc acgaacgccg cgcgcttcgg
     1021 gacggaaaag gcactggaac cgctgcgccg cgtgaatgcc gccgggaccc acctcctgag
     1081 cctcatcaat gaggtgctgg acctctcgaa gatcgaagcc ggcaagctcg agctcaatcc
     1141 cgagcgggtg aatttggccc gcctgatcga cgaggtcgtc ggcaccgcgg gacagctcgc
     1201 cgagaagaac cagaaccgcc tcaccgtcga agcgcaggag aacgttggtt cgctcaccgc
     1261 ggactccctg cggctcaagc agattctgct caatctccta agcaatgcct gcaagtttac
     1321 gaaagagggc gaagttgccc tgcgggtgcg caaggtggcg gatggccgtg gctgggtcga
     1381 gcttgccgtc gcggatagcg gcatcggcat gacggcggag cagcaggcaa aactgttcca
     1441 ggacttcacc caggccgatt ccctgaccgc gcggcgctat ggcgggacgg ggctcggcct
     1501 tgccctctcg cgcaagctcg cgcgcatgat ggggggcgac gtgaccgtga cgagcgagcc
     1561 gggcaagggc tcggtgttta cggtgcgtct gccagtgggc acggaaacgc actgacaaaa
     1621 tttcgcagac cgttccaacc gagaccggtg atctcgtgtc cactttgggt gctgggtcgg
     1681 catggctcta tgcggcggga ggcggcggtg gcggcttcgg tggtctcggc ggcgccggct
     1741 ttggtggctt tggcggcagc atggggcaac ccattttccg ttatggccgg cccggcggcg
     1801 gcgtgagacc tgacaatcct acacccacgg ggtgcccttc gggatcgcag cgaccttcat
     1861 tatgcggccg accacgcggg ccgttgaaga attgcttagt ggcagcgcag gctgatgtta
     1921 tgcttcgagg cactctggtg gattgtggag tcagtaccaa tgagtgaaaa cggcaccgag
     1981 acacccgaac cagtcgttaa atttgaaccg aaggcacgac cgcgcagtca tgacactccg
     2041 ctggatgaag ccggacaggc catcgttgca agaatccaaa gggccgccga gctagcgaat
     2101 gaaaactgcg accgcgccat gaagttggcg cataagcttg cgatgcagct cagagccgcc
     2161 gaagagcgaa taaatcaatt ggaagccgag gtcgaactct tccgagaccg ggcggcccgc
     2221 gccgaaggtt ggcttcaaac aatccaaaaa gagattgagg aaaagctcat cactccgaga
     2281 ttagcgtccg gcaccgaaca aaggtcgctc ccttagacca gctcgggttg tggattaacc
     2341 gacttgacgg tctcgctcgc cgcctgccgg ggcaaattca gatacgttca agctgtgtcg
     2401 atgaccgaac cctccgccac cgcaggacca tcgcgccgcg tcttgcgatc agcagacctc
     2461 caagggtgtc cccgtgccgc cgcgcaatca gtaaagctcg ttcggaatgc gttggctgta
     2521 tgctgcgtcg aacgcggcac tgtttatttc cgggtttcgc gccgccacga agtcgcccag
     2581 cgtcggcacg ccctgtggcc ggccgccact
//
LOCUS       DATLJT010000727         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3950487,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000727 DATLJT010000000
VERSION     DATLJT010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3950487"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VM910_16625"
     CDS             <1..289
                     /locus_tag="VM910_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI64189.1"
                     /translation="KQLRLVAADPHLNEMLLHYCEQALAYRRSREGPLQITIENAITP
                     LLPHGKAQLGAVAQTLGVSSRTLARTLAAEGLSFGEILSQLRSDLATHLSK"
     gene            412..558
                     /locus_tag="VM910_16630"
                     /pseudo
     CDS             412..558
                     /locus_tag="VM910_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            916..1848
                     /locus_tag="VM910_16635"
                     /pseudo
     CDS             916..1848
                     /locus_tag="VM910_16635"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1845..>2249
                     /locus_tag="VM910_16640"
     CDS             1845..>2249
                     /locus_tag="VM910_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64190.1"
                     /translation="MKRREFITLLGGAATWPVAARAQQVSTPVVGFLSSLRRDDDLAK
                     TYANGFRSGLSEVGYVEGRNVTIEYRFAENQRDRLPGLVTDLVGRKVAVIAATGGGAS
                     ILAAKAATSTIPIVFTFGADPVREGYVASLNRP"
BASE COUNT          422 a          669 c          726 g          432 t
ORIGIN      
        1 aaagcaactt cgtctcgtcg ccgcggatcc gcatctcaac gagatgctgc tccactattg
       61 cgagcaggcg ctggcatatc gccgctccag agagggtccg ctgcagatca ctatcgaaaa
      121 tgccatcact ccgctcctgc cccatggcaa ggcgcagctc ggcgcggtcg cgcagacgct
      181 tggcgtgagc agccggacgt tggcgcggac gctggctgcg gaaggcctga gcttcggtga
      241 aattctcagt caactccgct ccgatctcgc cacgcattta tctaagtgac cccaaccttc
      301 cgatctctga gatagcatgg ctcgttggtt atcagggagt tagcgcattc tcccatggct
      361 acaagcggtg gaccggaatg aacccgaaga agatgcgcgg caaattgctg gcgagccctc
      421 aagggcgcaa agcccgccga gctgccggtg caagagccga caaaatatga gcctgtcatc
      481 aatctcaaga ccgccagtgc gcttggcatc actgtgccgc caacgatgct cgcactggcg
      541 gacgaggtta ttgggtgaca gggttttgct gcggtgcatg agtccgcttt tgaccccaag
      601 cggacatgga gggtgctgaa agcacccgcg gccctacgtg cggccacgct agcgcgtccg
      661 gcggcaaagc aaggtgtctc ccatcgagcg attgcgatgc ctatgcgtat ggcagcgacc
      721 cctgagtgcg gcacgcaaga gcaaatcaat ggcaggaaat aacaaaacgg ctttctttcc
      781 ctcaacttac gatccttcgg gaatcgggct ggctgggaga ttccgcccgc gctgctcgcg
      841 cgcgccgatg aggatcgagt gaaacgccgc gagttcatta cgctcctcgg tggcacggct
      901 tccgcatggc cgctcatggc acgcgcacag caggtgcagc gcgtgcggct cgtctgtatt
      961 ttggaagggg tatcggcaga cacaccgggc tctaaggcgc gctatgcggc acttctggaa
     1021 gggctccagc aattgggttg gatcgacggt cagaacgtcc ggatcgatat ccggtgggcc
     1081 gaaggggatg cccgcgaaat tcgcagacac gcgagcgagt tggtggcgct cgcgcccgat
     1141 gtcatccttg cgagcggcac tgccgcgatg gagtcgttgc tgcgggccac gcgcaccata
     1201 ccaattgtgt tcaacaatgt agccgacccg gtcggcgccg gcttcgtcaa gagtatgtcg
     1261 cggccaggcg gcaacagtac cggctttatc cagttcgaat acaccttgag tgggaaatgg
     1321 ctggaactgc tcaagcagat cgctccgagc gtcgcgcggg tggcggtcct tcgagatgcc
     1381 tccataacgt cggggattgg ccaattcgct gtaatccaag ccgtggcgcc gtcggtcggg
     1441 gtggacgtga gcgcgatcaa cgtgcgcgat gccggcgaaa tcgagggcga catcgcggac
     1501 ttcgcgcgca cgtccaatgg cgcgctgatc ttgacggcta gcgcgttagc ggcggttcac
     1561 gccgagttgg tcatcgcgct tgcggcccgg cacggattgc ctgcggtcta ctggcgacgc
     1621 gccttcgtcg ctggcggtgg cttggtctct tatggtcctg atattgtcga ttagtaccgg
     1681 cgcgcggctg gatatgccga tcgcatcctc aaaggcgcgc ggcccgctga cctcccggtg
     1741 caggcgccaa ccaaatacga gctggtcgtc aatctcaaga ctgccaagag cctcgggctc
     1801 gaaattccga actccgttct cgcccgcgcc gacgaggtga tcgagtgaaa cgccgggagt
     1861 tcatcacgct gctcggcggc gctgcaacgt ggccagtcgc ggcgcgggcg cagcaggtgt
     1921 cgacgccggt ggttgggttc ctctccagcc tgcggcgaga tgatgacctg gccaaaacct
     1981 acgcgaatgg gttccgcagt ggcttgagtg aagtcggcta cgtcgaaggc cgcaacgtga
     2041 cgatcgaata ccgttttgcg gaaaatcagc gcgatcgatt gccggggctg gtgaccgatc
     2101 tagtcggtcg caaggttgcc gtgatcgcag cgacgggagg tggtgcatcg attttggctg
     2161 ccaaggcggc gaccagcact attccgattg tgttcacgtt tggtgccgac ccggtccggg
     2221 agggctacgt cgccagcctc aaccggccg
//
LOCUS       DATLJT010000728         2812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_505075,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000728 DATLJT010000000
VERSION     DATLJT010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505075"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="VM910_16645"
     CDS             complement(<1..1721)
                     /locus_tag="VM910_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI64191.1"
                     /translation="MEKKLEARTRELSEAREQQAATAQVLQVIASSPGELEPVFEAIL
                     ANATRICEAKFGMLYLWEGEGQYRVGALHGAPPRLAEERSRGTVIRPAPGSALGRVAQ
                     TKHTVHIADSRAEKNYTDVPPTFTPPGITIYGGARTQLGVPMLKEDRLIGVIAIYRQE
                     VRPFTDKQIELVKNFAAQAVIAIDNARLLNELRESLQQQIATADVLKVISRSTFDLQS
                     VLNTLTESAARLCNAYDAVILLREGAVLVFGAHYGPVPMGAEIGWPVSRGNVVGRSVV
                     DCKTVHVHDVTSESDEFPEGQAIARRIGVRTLLSVPLLREKEAIGTLLLRRTEVRPFT
                     QKQIELAETFADQAVIAIENVRLFEAEQARTRELTEALEQQTATSEVLGVISSSPGEL
                     EPVFRAMLENATRLCDAKLGIMFEFSDGAWRSLSSINVPRQYDEYCRQKRVWEPDTLL
                     GQVARTKTTAHIADVRTDRAYLERDPNRVASVELSGQRTAICVPMLKEHELVGAFGIF
                     RQEVRPFTDKQIELVSNFAKQAVIAIENTRLLNELRRRTNDLSESLEQQTAMSDVLRV
                     ISSSPTDL"
     gene            complement(1975..>2812)
                     /locus_tag="VM910_16650"
     CDS             complement(1975..>2812)
                     /locus_tag="VM910_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64192.1"
                     /translation="DTSARLMAQWLSDRLGQQFVVENRPGAGSNIATEAVVRARPDGY
                     TLLQASASNAWNAALYDNLNFNFIRDIAPVATVRRAGGIMEVNPSVSVRTVPEFIAYA
                     KANPGKINMASAGTGSAPHLFGELFKFMAGVDLTTVHYRGSGAALPDLIGGQVQVMFD
                     VVISSIGHVKAGKLRALGVTTAARLDTLPDVPPIGDFVSGYKAFSWDGIGAPAGTPPE
                     IIAILNKQVNAVLADPTFKARLVDLGAEPFISSPAEFGKFIVEFTEKWGKVIRTAGIK
                     GE"
BASE COUNT          544 a          868 c          794 g          606 t
ORIGIN      
        1 aggtccgtcg gtgaacttga gatcacgcgc aggacatcgg acatcgccgt ctgctgctct
       61 agcgactcgc tcagatcgtt tgtgcgccga cgcaactcgt tgagcaggcg cgtattctcg
      121 atggcgatca cggcctgctt ggcgaaattg ctgaccaact caatctgctt atccgtgaac
      181 gggcggacct cctgacggaa aatgccgaat gcaccaacca actcgtgttc tttgagcatc
      241 ggtacgcaaa ttgctgtccg ctgtccgctc agttcaaccg atgccacacg attcgggtcc
      301 ctttctagat acgcgcggtc agtgcgaaca tccgcaatat gggcagtggt cttagttcgc
      361 gcgacttgac ccaaaagtgt gtctggttcc cagacgcgct tctggcggca gtactcatcg
      421 tattggcgcg gcacgtttat cgacgagagt gaacgccacg cgccgtctga gaactcaaac
      481 ataataccta acttggcatc gcagaggcgg gtcgcattct ccagcattgc acggaatacc
      541 ggctcaagtt cgccggggga gctcgagatt accccaagga cttccgaggt cgccgtctgc
      601 tgctccagcg cctccgtgag ctcgcgcgtg cgcgcctgct ccgcttcgaa cagccgcacg
      661 ttctcgatcg cgatcaccgc ctggtcggcg aaggtttctg ccagctcgat ctgtttctgc
      721 gtaaagggtc gcacctcggt gcggcgaaga aggagagtgc cgatggcctc cttctctcgc
      781 agtagcggga ccgaaagaag ggtgcggacg ccgatccgcc gagcgattgc ctggccttcg
      841 gggaattcgt cactttcgga cgtgacgtca tgcacatgca ccgtcttgca atccacgacc
      901 gaacgtccga ccacgttccc tctgctgaca ggccatccga tttcagcccc cattgggacc
      961 gggccgtagt gagccccgaa aaccaggact gctccctctc gtagcaggat caccgcgtca
     1021 tatgcattgc atagccgcgc cgctgactct gtcagcgtat tgagcacgct ctgcaggtca
     1081 aaagtcgagc ggctgatgac tttgagcacg tcggcggtgg cgatttgctg ctgcagcgat
     1141 tcgcgcagtt cgttgagcag gcgtgcgttg tcgatcgcaa tgacggcttg ggcggcgaag
     1201 ttcttgacca actcaatttg cttgtcggta aacggccgca cctcctgtcg gtagatggca
     1261 atgacaccga ttagccggtc ctccttgagc atcgggacac ccagctgggt ccgcgctccg
     1321 ccgtaaatgg taatccccgg cggagtgaag gttggcggga cgtcagtgta attcttttcg
     1381 gcacgactat cggcaatgtg gaccgtatgc ttcgtctgcg cgactcgccc gagggcggat
     1441 ccaggggcgg gacggataac agttccgcgg ctgcgttcct ccgccagtcg aggcggcgca
     1501 ccgtgcaatg cgccgacgcg gtattgtcct tccccctccc aaaggtacag cataccgaac
     1561 ttggcctcgc agatgcgggt cgcgttcgcg agtatcgcct cgaacacggg ctcaagctct
     1621 ccgggcgagc tggcgatgac ctgcagcact tgggccgtag ccgcttgctg ctccctcgcc
     1681 tcgcttagct cgcgcgtacg cgcttcgagt ttcttctcca accctgctcg aggttcgcga
     1741 acgcgatcaa cgtgagttct cgccttcgtt gtcttcgaac gaagaccggt aacgcgtctt
     1801 cggctcttcg cccctttgcg agacaaggtc acctcgtcgg caggatcgac tgtttgccgc
     1861 acgctgagga tcagatctgc atcgaaatga tagcacagac acgtcaggtc tggatgaggc
     1921 ccttcacgcg accgggtgca tcgttttcgc gaacggaact tatggaatat ctcgtcactc
     1981 gccctttatg ccggctgtgc ggattacctt gccccacttc tccgtaaatt cgacaatgaa
     2041 tttgccgaac tcggcgggcg agcttataaa tggttctgcg cccaaatcga cgagccgcgc
     2101 tttgaacgtc ggatcagcaa ggacggcatt cacttgctta ttgaggatcg cgatgatctc
     2161 cggcggcgtg cccgcgggtg ctccaatgcc atcccaactg aacgccttgt aacccgacac
     2221 aaaatccccg atcgggggaa catccggcaa tgtgtccaaa cgtgcggcgg tcgtgacccc
     2281 tagcgctctc agctttcccg ccttaacgtg tccaatcgac gaaatcacaa cgtcaaacat
     2341 gacctgaacc tggccgccaa taagatcagg cagcgcagcc cccgagccgc gatagtgcac
     2401 cgtagtcaga tcgacacctg ccatgaactt aaataactcg ccgaaaaggt gcggcgcgct
     2461 ccccgtgccg gccgacgcca tgttgatctt tcctggatta gccttggcat aggcgatgaa
     2521 ctcgggaact gtcctgaccg agaccgatgg gttgacctcc attataccgc ccgcgcgcct
     2581 gacagttgcg accggcgcta tgtcgcggat gaaattgaaa ttaagattgt cgtagagtgc
     2641 cgcattccac gcattcgacg ccgaagcttg cagcagggta taaccatcgg gacgtgctcg
     2701 taccaccgct tccgtcgcga tattgcttcc cgcgcctggt cggttttcca cgacgaattg
     2761 ttggccgagc cggtccgata accattgcgc catcagccgt gcactggtgt cg
//
LOCUS       DATLJT010000729         3043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_45564,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000729 DATLJT010000000
VERSION     DATLJT010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45564"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="panB"
                     /locus_tag="VM910_16655"
     CDS             complement(<1..773)
                     /gene="panB"
                     /locus_tag="VM910_16655"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVI64193.1"
                     /translation="MAKNEARARAVSGPSGVAGKAAADSEKKREKRTITEIRESKRTG
                     EKMVYMSVPDYTSAKWAEMAGVDVAVVGDSLAMVAHGHPNTIPASMDMMIMHAQAVRR
                     GAPNTFVLGCMPYQSYNTIDRALVNATRFMQEALSDAVKPQDGKSQAHILRALVDAGI
                     PTASHIGLTPHTIAMFGGFKIQGRRAEAAMKILEDALAIEDAGCFMLEFEAVPAKIAK
                     LISEQLTIPTIGIGAGAGTDGQILLCYDLLGVFTDFKPK"
     gene            1008..1532
                     /locus_tag="VM910_16660"
     CDS             1008..1532
                     /locus_tag="VM910_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI64194.1"
                     /translation="MAIYQSMQRVRFRSAGDYEQFKLIFAGVRHHLKTLPGFLHLTWW
                     THPDDPTWHNEVSFWTSFDALKDWHMNTYHKHAKQWAVRSGAIMEDIITNFELKSTRL
                     IRVCPCCGHAQDKPYELDQEQHALAQPCSQCGFHFPRMPETPDSFAVFKDITHDAPIK
                     EAGERKPARAKSAA"
     gene            complement(1620..2153)
                     /locus_tag="VM910_16665"
     CDS             complement(1620..2153)
                     /locus_tag="VM910_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI64195.1"
                     /translation="MAGLDSSAAVIYLGTFSKSIGAGLRVGYLVVPVELAETAITVKA
                     ILNAGQSWLDQAVLAEFLSSGAFARHLKQVRMVYRSRRDCILSALREHFGDVNVSGHN
                     GGMHIMWHLSARLPHATVVEATALNHGVGIYALRSAVAYDFGKTPYSERSLVLGYAAL
                     QEKQICEGISRVAAALK"
     gene            complement(2137..3027)
                     /locus_tag="VM910_16670"
     CDS             complement(2137..3027)
                     /locus_tag="VM910_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVI64196.1"
                     /translation="MGEQIRFLISGGKLRPGCYIPSTRQLAEHLGVSRNTVLLAYQRL
                     ASEGYIDATKGSHTYVSASHAMSVVPRADTRSPSPASSAAIRRPPILFHGEAQRLVHL
                     GRKPLFDFFVGRPCPKCFPRQAWRRLLLQRLASAGSSLSEYQHPAGLLELRHAIAEHL
                     GPARGMNVRAAQVIIVNGIQEALNIIARLFVKPGTPVAIENPCYRGALSTFESYGAEM
                     IPVQVDEDGIRVDRLPERVVSLIYVTPSHQFPTGETLTFERRVELLNWATRVGAYVVE
                     DDYDSDSIRWTSADSRGGPG"
BASE COUNT          589 a          967 c          916 g          571 t
ORIGIN      
        1 aatttcggct tgaaatcggt aaacacgccg agcaggtcgt aacagagaag aatctggccg
       61 tcggtgcccg cgccggcgcc gattccgatc gtggggatcg tcagctgttc ggaaatcaac
      121 ttggcgatct tcgccgggac agcttcgaat tcgagcatga aacatccggc gtcctcgatc
      181 gcgagcgcat cctccagaat cttcatcgcg gcctcggccc ggcgaccctg gatcttgaat
      241 cccccgaaca tcgcgatcgt gtgaggcgta aggccgatgt gggaggccgt cggaatgccg
      301 gcatcgacga gcgcccgcag gatgtgcgct tgcgacttgc cgtcctgcgg cttcaccgcg
      361 tccgacagcg cctcctgcat gaagcgcgtg gcgttcacca gcgcgcggtc gatcgtgttg
      421 tagctctggt aaggcatgca tcccagaacg aaagtattcg gggctccgcg ccgaacggcc
      481 tgcgcgtgca tgatcatcat gtccatcgag gcgggaattg tgtttgggtg tccgtgcgcg
      541 accatcgcaa gactgtcgcc aacgaccgcg acgtcgaccc ccgccatctc ggcccatttg
      601 gccgaggtgt agtcgggcac ggacatgtag accatctttt ctccggtcct cttcgactcc
      661 cggatttccg tgatggtccg cttttcccgc tttttttcgc tatcggcagc ggctttgccc
      721 gcaacgcccg acgggccgga cacagcgcgc gccctcgctt cgttctttgc catggccagc
      781 ccgacgagca acgtccgcga tctgatatac ttgttgtatg ttagagagga tgcgtcgcgt
      841 tggtcaagcc gaggcggctg gcgcatagcg ccgcccagga cagcgacgcg ctggttccat
      901 tagattttcg ccgactggct cttttgcttc gcccgtcgga gccgaagatt gaacgcatat
      961 gcgcgaccgg ccctcccgcc cgcgccatcc gtggggagaa cgatatcatg gccatctacc
     1021 agagcatgca acgcgtgaga ttcaggagcg ccggcgacta cgagcaattc aagctcatct
     1081 tcgcgggcgt gcggcaccat ctcaagactt tgcccggctt cctccacctg acctggtgga
     1141 cgcaccccga cgacccgact tggcacaatg aggtcagctt ctggaccagc ttcgacgcgc
     1201 tcaaggactg gcacatgaac acctaccaca agcacgccaa gcagtgggcg gtccgttcag
     1261 gcgcgatcat ggaagacatc atcaccaatt tcgagctcaa gagcacccgc cttatccgcg
     1321 tctgtccgtg ctgcgggcac gcccaggaca agccctacga attggatcag gagcagcacg
     1381 cgcttgccca accatgttcg cagtgcggct tccacttccc caggatgccg gaaacgcccg
     1441 acagctttgc cgtgttcaag gacatcacgc acgacgcgcc gatcaaagag gcgggggagc
     1501 gcaagcctgc aagggccaaa tcggcggctt agacctgagc tagtttgggc ccgtgtcccc
     1561 gctcctgtcg ggacggccgg gcccgcgccc cggcccttcg gcgaacacgt gcggggcgcc
     1621 tacttcagcg ctgccgcgac tcggctgatc ccctcgcaaa tttgcttctc ctgcagcgct
     1681 gcgtagccga gcacgagcga gcgctcgctg tacggcgtct tgccgaagtc gtaggcaacc
     1741 gccgatcgca aggcgtagat gccgactcca tggttcagcg ccgtggcctc gacgacggtt
     1801 gcgtgcggga gccgcgcgga cagatgccac atgatgtgca tgccgccgtt gtgtcccgat
     1861 acgttcacgt cgccgaaatg ctcgcgaagc gcactcaaaa tgcaatcgcg gcgggaacga
     1921 tagaccatcc tgacctgctt gagatggcgg gcaaaggcgc cgctcgagag gaactcggcg
     1981 agcacggcct gatccaacca gctctgcccg gcgttcaaga tcgctttgac cgtgatggcg
     2041 gtctcggcga gttccaccgg aacgacgagg taaccaactc tgagtccggc gccgatcgat
     2101 ttcgagaatg tcccgaggta aatgacggct gcggagctat ccaggcccgc cacggctgtc
     2161 agcggaggtc catcgaatcg aatcgctgtc gtagtcgtcc tcgaccacat acgcgccgac
     2221 gcgcgtggcc cagttcagca gctccacgcg ccgctcgaat gtcaacgttt cccccgtggg
     2281 gaactgatgc gacggcgtta cgtagatcag actgacgacg cgctcgggaa gacgatcgac
     2341 ccggattccg tcttcatcaa cctgcacggg aatcatctca gccccgtagc tttcaaatgt
     2401 cgagagcgcc ccgcgatagc acggattttc gatcgccacc ggcgttcccg gtttgacgaa
     2461 cagccgagcg atgatgttga gagcttcttg tattccattc acgatgatga cctgcgcggc
     2521 gcgaacattc atgccgcggg ccgggcccaa atgctcggca atggcgtgcc tgagttccag
     2581 aaggccggcg ggatgctgat attcggaaag cgatgagcct gcggaggcta ggcgctgcag
     2641 caggagccgc cgccacgctt gccgcgggaa acacttcggg cagggccggc ccacgaaaaa
     2701 atcgaacagc ggctttcgac ccaaatgaac caatctctgg gcttcgccat gaaaaagaat
     2761 cggcgggcga cggattgccg cactcgacgc cggcgagggg cttcgcgtat ctgcgcgcgg
     2821 taccaccgac atcgcatgcg aagcactgac ataagtatgc gaaccctttg tcgcgtcgat
     2881 gtagccttca ctggcgaggc gttggtatgc caatagcacc gtatttctgg agacaccgag
     2941 atgctcggcc aactggcggg tactgggaat atagcaaccc ggccgcaact tgccgccgct
     3001 gatcaagaag cggatttgct cgccgatttg ctcgtgaagc gcg
//
LOCUS       DATLJT010000730        17249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3721283,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000730 DATLJT010000000
VERSION     DATLJT010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17249
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721283"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..529)
                     /locus_tag="VM910_16675"
     CDS             complement(362..529)
                     /locus_tag="VM910_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64197.1"
                     /translation="MSTTIWSGGAAAGLYLGTGLILAFLVHTHYPGVLGLREWIRVVL
                     SWPLIFFLRSK"
     gene            complement(526..1326)
                     /locus_tag="VM910_16680"
     CDS             complement(526..1326)
                     /locus_tag="VM910_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64198.1"
                     /translation="MRRKIACAIIALTMVGIAVQASMAASFDVVTDFNNTGVQPAPGD
                     PHSFPFTYGTETALNVGFTLLPFFGNTNCSVASGQCTNDGTVDNYYFVQPEQWSGPAI
                     GKVASGGTLTFPGIPGGWHVPDNVLVMMPGGSSFPSPDLVVTRFTAPNAGTFNITGSL
                     TDLESAGIRFTILVDGASVFSASFAGLSLLQGTIPFTIDDVRLQPTSTVDFVVDSLGA
                     QGNDVVGLMATISPSVSVPGPIAGAGLPGLIFAGGGLLGWWRRRRRTA"
     gene            complement(1610..1891)
                     /locus_tag="VM910_16685"
     CDS             complement(1610..1891)
                     /locus_tag="VM910_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64199.1"
                     /translation="MAIGDQDCGRVAVTVSGMLEGNILQIGVGRRTISCAGEKPCGDS
                     NQGPTSQTSQLVQAMAGFGGGSGAAENLNAARLSTDTSQQMLLSTPQHA"
     gene            2724..3047
                     /locus_tag="VM910_16690"
     CDS             2724..3047
                     /locus_tag="VM910_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64200.1"
                     /translation="MIWIERQAIHPHASVVAEPFHVLFAGVVTALAQALQFPEPELVD
                     VTAPRLNVVRYVSSSHFAFGQARPAQRLDPQLMLGYPSPTLGSIKAHRIPPCGDCLHT
                     VSAGP"
     gene            complement(3300..3683)
                     /locus_tag="VM910_16695"
     CDS             complement(3300..3683)
                     /locus_tag="VM910_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64201.1"
                     /translation="MRTVTAARRGRQVEPHPVPLTRAWVEDYRRYFDEQLRAPAVLAE
                     VCELSALCGLASGLVGRLKAVDRGSCHCGRPGAFIEQRANGWVWYCLDHRPDFRACHI
                     CGQPGIAFEYAGERWLWYCRAHVTG"
     gene            complement(3895..6267)
                     /locus_tag="VM910_16700"
     CDS             complement(3895..6267)
                     /locus_tag="VM910_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HVI64202.1"
                     /translation="MMLLEPNRDQIEIFVQALFRYVGKDGFISMRAFYENDDSDKPVR
                     ITPTSLAGGLGFVIDVAEDDARRAANQPRATVFCPPVAVFNNKDHAREVDLLIGPALV
                     VECDEDPQQARVKLEQLLGTATLVVRSGGQWTNPTTEQVYDRVHLYWRLAQGAKGDNL
                     EKLKRARKLATYLVGGDTTNVPICHPIRWPGSWHCKNAPRLCEIVELHPDCEIDLDRA
                     LVVLTTAAPNVSTTKSGDPGRDDGEALDWSKSFGEIITGEHFHPTLVPLAASFAAHGL
                     PELAARGVLRALLDSTQTKDPERLRRRDTELGKLRDTVHSGYKKFAAAVPTDTLLDPW
                     AQAQLPDFPLATLPVIVGRFVERQSLIIGGCPSAMALSVLCALSGAIDHRFKVKMMRH
                     GDWEARPRLWGLLVGDPSKKKTPIINAAARPLERWQRRLWREYQAQLRDHIAAGGKAD
                     DEDAPQEPLHYVVYDTTIEALGDALAKSSRGVLVKRDELAGWIASMERYGGAARGGSD
                     RGFWLQVYDGEGYGFLRIKRGNIPISDFSVSILGGIQPARLAELQGLTSDGLLQRFLV
                     AMISMANRGQDIESNATEYGLLVERLLSTPRQRLELTEDALACLNGLLDYLYSLEQMG
                     DGGADGFQSFVGKLAGYAGSLATILHVAEEHGPTLPNFIGSTIIKRVDQIIREFVIPH
                     GHEFYRLGGAAEQLKQLASFILTSDLERFRAADLASNVRDLKGQSLVTINERLSPLIA
                     GGWLDPADRTQVCRVWTLNPSVRVRFADRRRTEVIRKKAVTTMLRERRAS"
     gene            complement(6264..7691)
                     /locus_tag="VM910_16705"
     CDS             complement(6264..7691)
                     /locus_tag="VM910_16705"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI64203.1"
                     /translation="MSDIELVPDLLSPATPAVPLVLRPYQVDDLARLRAQFRSGVRRV
                     CYQGPCGCGKTVIFSEVVAGAVRRGNRVCILGHRDEIIQQITRALDALGVAHALVVAG
                     CDLAALEHPVQVASVQTLVRRLKHLMPPDLFVIDESHHAAANTWRRILAAFPQAKVLG
                     VTATPQRLDGKGLDDIFDALAIGPSVSELIAAGYLSLFATFAPARSPDLSGIRTRAGD
                     YATDELAARMSKGVIISTAVDEYTRLCAGRAAIVYCVDIQHSELVTEAFRARGYRAAH
                     LDGNTPTELRRSIIAALGSGELQIVSNCGIISEGLDVPGVVAVILLRPTKSLALHLQQ
                     IERALRPAPGKAKAFILDHASNTFRFGPADAPQNWTLKGREKGEQPLPLQRCDHCGAL
                     VPLACLECPECGAVLREPPGPRTHVERRGEALVLADRLSTMSYWQCLRWAGHDKDRLR
                     LVATARGYKPGWVWHRLQELQEGEQ"
     gene            complement(7688..8269)
                     /locus_tag="VM910_16710"
     CDS             complement(7688..8269)
                     /locus_tag="VM910_16710"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HVI64204.1"
                     /translation="MAEHELAVPIEPLEWFTPQSTFDGIRRGGIEEFDLDAAHPGRSN
                     PYCIVPARKIYTIADDGLRQPWHGLVWLNPPFGGRRGQVPWLRKFFAHGNGIALCAAR
                     TSADWFHEVVVPNAQLLLFPSGKTKFIRPDGSIGKEPGTGVVLIGAGRVACEALLQSG
                     LGACMTIVTRSVSACERRPAAWGNEIDHFGGGA"
     gene            complement(8262..9011)
                     /locus_tag="VM910_16715"
     CDS             complement(8262..9011)
                     /locus_tag="VM910_16715"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HVI64205.1"
                     /translation="MQRTSGYGHGLDETPDAACVAPAPFAALRLNVAQQSDALVLLRL
                     LPDGYAPLVFFDPQHRAIMDKKKYGNEGERQRGRAKLPAMSEDYIDACCIESARILKP
                     SGYLMRWMDKFCLCEGHHLRISQQLLERVDLIAWDNLRPGQGDRCRNRGDYLLALQKP
                     PKRARATWRDRGIWCRWVEKIDLKRYPRKLYPHTKPIGLIKRLIAATTQPDDVIIDPC
                     AGSFVVLHAALELGRNFFGCDLKLPDGGAHG"
     gene            complement(9078..9491)
                     /locus_tag="VM910_16720"
     CDS             complement(9078..9491)
                     /locus_tag="VM910_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64206.1"
                     /translation="MTSISERYGSSLMTAQDVKAGNLTLQISYVEWDVLMGKETRDLV
                     HFVGEDRCLVLNPTNARALARLLGDDGDKWRGNWITLYYDPDVEYQGEKKGGIRVRCA
                     TPPPTAGNGPLPEPPPPRKPPARAVDAVEEEEAPF"
     gene            complement(9717..10658)
                     /gene="dnaN"
                     /locus_tag="VM910_16725"
     CDS             complement(9717..10658)
                     /gene="dnaN"
                     /locus_tag="VM910_16725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVI64207.1"
                     /translation="MKFEATAGNLSAALGAAALALDDKSNVAALSMARIVVRDSVVFV
                     VDSLDRRITTNVTATIVEPGEVSARCTALAGVLGGLDPKRTVTVESSGNGIIVRAGRS
                     RFTIAGLPIDILPQSSELADPATSFDLDADQLRRMLEVCEHAISTEEMRYYLNGLYLH
                     TADKTLRGVATDGHRLCWFDLPMPAGADEMSGIIVPAKIVAILIKLLKRKPAPETVSV
                     RLSDRLVEILLPGLALTAKLIDGTFPDYARIIPPASGNTVTVDTDNLRQALARIDALF
                     DQKMKHVMRTAGLAWSNEALHVSMTGTDENRRHYRGR"
     gene            complement(10664..10879)
                     /locus_tag="VM910_16730"
     CDS             complement(10664..10879)
                     /locus_tag="VM910_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64208.1"
                     /translation="MTTMPKFKTDTPTTTTDTAFIGGCAIRLFGDLRCPRCRAQLRAS
                     AMRDIGDGEYTLTCQRCHADAITITDR"
     gene            complement(10900..11736)
                     /locus_tag="VM910_16735"
     CDS             complement(10900..11736)
                     /locus_tag="VM910_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64209.1"
                     /translation="MHDDSNVRTLTTRYQQILDAYKRMWESIPVDADGCKHAPVCDDE
                     MPDYLSHLVGRTVTAAEWQAAQRWEWEQIAGGKIVAALPRPAEVSVQIDDYQDALQRL
                     IKRLVQLLGHPTIANLVFADYEFNEDQYWLHTLGELEAAAKLMLEFAKQAPATLYVMR
                     GSLERLVLAPDLVMNEATRTAYRATIEALDRTQAALGVPLAERSRHFEVKRIHDAPAP
                     PPPPDANPELQQMSSDELRAALTDTRIRSEAIETEISRRGTAAARALAVMLVSAREPP
                     AR"
     gene            complement(11729..13084)
                     /locus_tag="VM910_16740"
     CDS             complement(11729..13084)
                     /locus_tag="VM910_16740"
                     /inference="COORDINATES: protein motif:HMM:NF040299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6011 domain-containing protein"
                     /protein_id="HVI64210.1"
                     /translation="MKFAGGKHANWSRCHRPQQLALSSSAGSEHMITTAEASRKIRAA
                     LRTAFPGTKFAVALSAGCSITWTDEGPSVVQVEDALLAAGCGEAKISWDDKRYIRIDT
                     DRSNDTLYFDRFNAAERAAYQQHLERRREEERAFAQHTAEVIRDAYKAKRAAIAPLKD
                     HTPPPIQDATVFEAFERLRQRAEAEVSITEDAERRPSWAPPLVLEEELANICYELGYL
                     TDDNKWIARFWATFATPKRSGRWLREHVSNLPLTGLSCRGFQLHAGGTRGSTGNILFE
                     AQREESGTWRFGPREYFFPYFSPKQREWERLIGERERAPTIYNDRQLGTRLAEIARQI
                     EAIDAEDLTKAKAHRDRQRLRQRALELARARVLDFIGAPDAQMQLAARLCGHCCICFK
                     ELTDPISLEHGIGPDCHAKRIKFIHARAADGWPLERIVMLSGMPVDFVTSVLSEEVRH
                     A"
     gene            13149..13529
                     /locus_tag="VM910_16745"
     CDS             13149..13529
                     /locus_tag="VM910_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64211.1"
                     /translation="MVERLFDLLSDAAWVAIPMRCWVATNNFLSQILGSGAQVLQQVP
                     RGNTLAFAECMRQIVDERLSGFNLSDEVLDHDLSGSLSVFEGVNGHDVSPEKSCVTVD
                     SVVASERVPRVRDARFCSIRSRAP"
     gene            complement(13516..13941)
                     /locus_tag="VM910_16750"
     CDS             complement(13516..13941)
                     /locus_tag="VM910_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64212.1"
                     /translation="MLYLANGGRSVNATFNLRGLALRHLPEEKRVALAVDTRLGLLQP
                     TGFRTKDVCSMYGVSARAVNVGIREWAIANGGQQLAGITADVINSEGNGTGNGTSHGS
                     TDSAIQFAEALKTMNPDELATSFKIAGSEVIWAALVRAL"
     gene            complement(14080..14598)
                     /locus_tag="VM910_16755"
     CDS             complement(14080..14598)
                     /locus_tag="VM910_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64213.1"
                     /translation="MARRKTIKQKAAPAQTALSKKAAALKARTAAPALSPKLAAANTK
                     YRLEAGLAVAVPSHDHDCEHVHAARAPPSARFSDKAEAMAKRPARTKTTQRARFRGLQ
                     SGAFTISSFCVAHDLSESFYHKLRNRGLGPREMRIGARVLISHEAASNWRKAREKATL
                     RAKPSPSPEAAE"
     gene            complement(14696..15232)
                     /locus_tag="VM910_16760"
     CDS             complement(14696..15232)
                     /locus_tag="VM910_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64214.1"
                     /translation="MFQLSLSVDVWTDDDGCERLKLWAATDMVGNGDHQACVGGYERA
                     RAMEADQSARKWQVSAAGITALLKESNTELIAEKITAAIAGLPAPKDGIDVEAVKQLI
                     DEKFAAAAKTSPQQRRKPGPKKHQGWKLVIAWLAYDHKKKHGAFPSSGTLGELCEAEM
                     GYMPDPSAINKLLRDLAD"
     gene            complement(15581..16840)
                     /locus_tag="VM910_16765"
     CDS             complement(15581..16840)
                     /locus_tag="VM910_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI64215.1"
                     /translation="MARTVRDAALESRTARERLAPRGEPYYKALEPGLLHLGYRKPKS
                     GTGKWLARCYVGNHSYRYHPIGLADDFSDADGRVILSYSQAQKAARKLMVELAGGGIG
                     TIADTTAAYLKSLEAEGRPPAALSRTRYNIDALILPVLGDVELTKLTYEQLKAWFDGL
                     VRKPARRKGRRDLDETDADRARRATANRIRTTLVAALNVAFRAGHVSSDHAWRRLRPF
                     RGVDKARVRYLSVAEAKRLINAASPDFRQLLQGALLTGARYGQLAQLTVSDLNTDAGT
                     LRLRTRKGDGSERAYHAHLSDEALAFFRAAVAGRPGKDLIFTHADGTPWEKSHQDIPI
                     EEASRHAGISPPVNFHVTRHTFASHAVMNGAPLLVVAQALGHTDTRMVEKHYSHLAPS
                     YAADAIRKAAPIYGIKPGNVRPLHGKA"
BASE COUNT         3304 a         5273 c         5225 g         3447 t
ORIGIN      
        1 gacacctcaa acaaatatcc atagttcggc gcggtctgct gctccgcgaa cacgcgctcg
       61 cttgcccaat caacctcaaa gccattgatg atgcgcttca taatggccgc tccagacgag
      121 gccactatca cctcagccgc atgcggtagc tcaagccgtc tgatcgggaa tggtatgccg
      181 gatgatggct tcgagtgatg tgatggccat cacgaaatgg tcgattcagg ccccgtcgtc
      241 caagaaaagc cccgccttcg gtgagcgggc ctaagacgat cagcaatgtt cttgcggaag
      301 aacgcccctt tctttccgcg cgaatcggga tcgcgatgcc aaagagcaat ggcgataacc
      361 tctacttgct tctcaagaag aaaatgagtg gccaagagag gaccacacga atccattcgc
      421 gcaacccgag cacaccgggg tagtgcgtgt gcaccaaaaa agccaagatc agcccggtgc
      481 caaggtagag tccagccgcc gcaccaccgc tccagatcgt cgtgctcaag cagtcctccg
      541 ccgccgtcgc caccagccga gaaggccacc gccagcaaag atcaggccag gaagtcccgc
      601 gcccgcgatg ggaccgggca cagacaccga aggggaaatc gtagccatca acccaactac
      661 gtcattgccc tgtgcaccca agctgtcgac aacaaagtca acggtcgatg tgggctggag
      721 acggacatcg tcaatcgtga acggaattgt cccctgaagc agggacaagc ctgcaaagga
      781 cgcgctgaat accgacgctc catcgacgag tatcgtgaaa cggataccgg cgctttctag
      841 atcggtcaag gaaccagtga tgttgaacgt gccggcgttc ggagcagtga accgtgtcac
      901 cacaagatca ggagaaggga acgacgaacc acccggcatc atgacgagca cgttgtctgg
      961 aacgtgccag ccgccgggaa tacctgggaa ggtcaaggta cccccgctgg cgaccttgcc
     1021 gatggcaggc ccagaccatt gttcaggttg gacgaagtag taattatcga ccgtcccgtc
     1081 attcgtgcac tgaccggacg cgactgagca gttcgtattc ccgaaaaagg ggaggagcgt
     1141 gaagccgaca ttcaaagccg tttcggtgcc gtaggtaaat gggaagctat gtgggtcacc
     1201 gggtgcaggt tgaacgccgg tgttgttgaa atcagtaact acatcgaaac tggcggccat
     1261 cgacgcttgg acagcgatgc caaccatcgt cagggcgatt attgcacatg caatttttcg
     1321 tctcatggca cactcattta gccgtgcatt cggcagtcgc tggcgtgaca aggagggcca
     1381 tacaagcgaa agcgccccat gctgcggccg tgctgagtag cgctgcgtct tcaatttcat
     1441 gattgctccc ctctcgacga accgccgcgg ccaccacgag cgtgggtttc aggacaacca
     1501 tgtcaattgc tgttttttgg acggcgtaaa ctaggcgtaa aaatacgaag ccattgcgtc
     1561 accgcagagt aggcgcgatc tgcgcgcatt aatgagtaca cgcccagcct catgcatgct
     1621 gcggcgtgct cagcaacatc tgctgtgacg tgtcggtact gaggcgagca gcgttcaagt
     1681 tctcggccgc gccgctgcca ccgccaaagc cggccatcgc ttgcacgagc tgagaggttt
     1741 gagaggtcgg gccctgattg gaatcgccgc agggcttttc tcccgcgcat gagattgtcc
     1801 ggcgcccaac gccgatttgc agaatgttgc cctcaagcat tcccgatacg gtcaccgcga
     1861 cgcgcccgca atcctgatcg cctatcgcca cgagctacgc gcaagcgaac tttgtgaatt
     1921 gacctggaat atgatcgagc ttgatagcgg ccgcatccat gtgcgacgaa ccaagaacgg
     1981 tgtcgatggc acgcacccgc tcaccggcaa ggagatccgg gccctacgcc agctgcggcg
     2041 gggagaatct gcaaagccca ctgccgggct ttcctcttgc aactcgcgaa tggcccggcc
     2101 acgccgtcgg cgctcaacca gctctagagg agagcggtgc agaatttaca cgtcagcgcc
     2161 accttggcca caaattgcac acagcgagaa ccggtcgagg gcaaggccaa tcactctaaa
     2221 gggcgctctc gcggtcacgc gaccaaactt tccggatcga tcacccggac ctgcttgcaa
     2281 caactccggg gcaggaatct gaacgtccgc tgatgatccg gtctgggcgg cggctcagaa
     2341 tgcatgcaag ccagaaaggt ctgggcaatc tcctcgaccg cccgctcgcc cctgctgcag
     2401 gccaagcagg gcagcagttt gatcgtccgc ggaatgagct cggtgccgac gggatctact
     2461 ttggtgctgc gacgaggcat ggcatttccg cgaggcgaac gcgaggagaa cgcgactcat
     2521 caggcgcctt ttttgaccct catgcaagct tggcacagat gatgaacgtc catcgttttt
     2581 tgggaaaaat ttgctggcgg aaaattttcg tgagtttgca ttatttcgag ctcccccgcg
     2641 gtcgggcgcg agcatagttg aattcgcatt ccattccccc ctggcccctg gttttgacag
     2701 gtgggttaca tccgatagac gggatgattt ggatcgagag gcaggccatc cacccccacg
     2761 cgtcggtcgt agccgagccg ttccacgtcc tttttgctgg agttgtgaca gcgctcgcac
     2821 aagctctgca atttcccgag ccagaacttg ttgacgtcac cgcgccacgg ctcaatgtgg
     2881 tccgctacgt gagcagcagt cactttgcct tcggccaagc acgtccggca cagcggctcg
     2941 accctcagtt gatgcttggc tatccgtcgc caacgctcgg ttccataaag gcgcatcgaa
     3001 ttcccccttg cggcgactgt ttgcataccg tgagcgccgg accgtgagta tccggcacga
     3061 actcaatgca acagcgcgtc cggatccgcc gcgcgctcga tgacagccat ctcaacatcc
     3121 aagtgttgac ggtagccgac cgcgatatta gcgagatcgg cctgcagaag tgcgagctcc
     3181 gcctcccact cgcggcgcgg aactgcttgc gagcccgcat ctgggcgcag agaaaaccca
     3241 agcggtaggc gcgttggaga gggtgcattt ggcgcatcga aggcctcggg aattctacgc
     3301 tacccagtta cgtgcgcccg acaataccaa agccagcgct cgccggcata ctcgaacgca
     3361 atacctggct gaccgcagat gtgacaggcg cggaagtcgg ggcggtgatc taggcagtac
     3421 cagacccaac cattcgccct ttgctcgatg aaggcaccag gcctgccgca gtgacagctg
     3481 ccgcgatcga cggctttcaa ccgcccgacc agcccggaag cgaggccgca cagggcggag
     3541 agctcgcaca cctctgctaa gaccgccggc gctcgtagct gctcgtcgaa gtaccgccga
     3601 taatcctcga cccaggcgcg ggtgagcggg acgggatggg gctcgacctg cctgcccctc
     3661 ctggcggccg tcacggttcg catggctcgc atcctctttc ctgacggatt cagcctaccg
     3721 ccggttgtgg tctcagcgtc ggacagatcg gacggtcgga cgtgaggcct taggtgcgag
     3781 agggaacttt ttaaaagaga ggggccgcgt tggtgcaatt ttctgttcac ttacgtgcgc
     3841 ggtccgatcc gtccgacagt gtccgatttt tctatctcca gttgccgttt ttggtcacga
     3901 tgcacgcctt tcgcgcagca tggtggtgac cgcttttttg cgtatcacct cggtccggcg
     3961 acgatcggcg aaccgaaccc tcacgctcgg gttgagtgtc caaacccggc agacctgcgt
     4021 ccggtcggca ggatccagcc agccgccggc gatcaggggc gagagccgct cgttgatcgt
     4081 gacgagggac tggcccttca ggtcccggac attggaggcg agatcggcgg cgcggaagcg
     4141 ctccagatcg gacgtgagga tgaagctcgc aagctgcttc agttgctcgg cggcgccgcc
     4201 taggcgatag aactcgtgac catgcgggat gacgaactcg cggataatct ggtctacccg
     4261 cttgatgatg gtcgagccga tgaagttcgg cagcgtcgga ccgtgctctt cggccacgtg
     4321 aaggatggtg gcgagactgc cagcataacc ggccagcttg ccgacgaagg actggaaccc
     4381 gtccgcgccc ccgtcgccca tctgctcgag gctgtacagg tagtcgagca gcccgttgag
     4441 gcacgcgagc gcgtcctcgg tcaattcgag ccgctggcga ggtgtagaca gcagcctctc
     4501 gacgagcaga ccatattccg tcgcgttgct ctcgatatct tggccgcggt tggccatcga
     4561 tatcatcgct acgaggaaac gctgtagcag cccgtctgag gtcaaacctt gcagctccgc
     4621 gagccgcgcc ggttgaatgc cgccgaggat gctgaccgag aagtccgaaa taggaatgtt
     4681 gccgcgcttg atgcgaagaa acccgtagcc ctcgccatca tagacctgca accagaaccc
     4741 acgatcactc cctccgcgcg ctgctccacc gtacctctcc atgctcgcga tccaaccggc
     4801 gagctcgtca cgcttgacca agacgccgcg agaggacttt gccaaagcgt caccgagcgc
     4861 ctcgatcgtt gtgtcgtaaa cgacgtagtg tagcggttcc tgcggagcgt cttcgtcatc
     4921 cgctttgccg ccggcggcga tgtggtcgcg caattgagcc tgatactcac gccagagtct
     4981 gcgttgccag cgctccagcg gccgagcggc ggcatttatg atcggagtct ttttcttcga
     5041 tggatcgccg acgagcaaac cccacagccg cggccgtgcc tcccagtccc cgtggcgcat
     5101 catcttgact ttgaaccggt gatcgatggc gccggacagg gcgcagagca cggagagcgc
     5161 catcgcgctc ggacagccgc cgatgatcag gctctgtcgc tcgacgaagc gcccgacgat
     5221 caccggcagc gtggcgaggg ggaagtccgg cagctgagct tgcgcccagg gatcgagcaa
     5281 cgtgtcggta ggaacagcgg cagcaaattt cttatagcca gaatgcaccg tgtctctgag
     5341 cttgccgagc tcggtatcgc ggcgacgaag gcgctcagga tctttcgttt gggtgctgtc
     5401 gagcagcgcc cgcagcacgc cccgcgccgc caattcaggc agtccgtgtg cggcgaagct
     5461 cgctgctagc ggcacgagcg tggggtgaaa atgctcgcca gtgatgatct cgccgaaaga
     5521 tttgctccaa tcgagcgcct cgccgtcatc gcgccctggg tcacccgact tcgttgtcga
     5581 cacgttcggc gctgctgtcg tgagtacgac cagcgcccga tcgagatcga tctcgcagtc
     5641 gggatgcagt tcgacgatct cacatagccg cggcgcgttc ttgcaatgcc acgagccggg
     5701 ccaacggatc gggtgacaaa ttgggacatt cgtggtgtcg ccaccgacga gataagtcgc
     5761 gagcttgcgc gcgcgtttga gcttctcaag gttgtcgcct ttggcaccct gagcgaggcg
     5821 ccaatacaaa tgcactctgt cgtagacctg ttcggtcgtc ggattggtcc attgcccgcc
     5881 actgcgtacg acgagagttg cggtgccgag caattgctcg agcttgacgc gcgcctgttg
     5941 cggatcttca tcgcattcaa ccactaacgc tgggccgatc agaagatcca cctcgcgggc
     6001 atggtccttg ttgttgaaga ccgccaccgg cggacagaag actgtcgctc gaggttgatt
     6061 ggctgcgcgc cgcgcatcgt cttcggcgac gtcaatgaca aaacctaacc caccggccag
     6121 gctcgtcggt gtgatccgca ccggcttgtc cgaatcgtcg ttctcataaa acgcgcgcat
     6181 actgatgaag ccatccttgc cgacatagcg gaacagcgcc tgaacaaaaa tttcaatctg
     6241 gtcacggttg ggttcgagca gcatcattgc tcgccctcct gtaattcctg caagcgatgc
     6301 cagacccacc ccggcttata gccgcgcgcg gtggcaacca gacgcaaccg gtccttgtca
     6361 tgtccggccc aacgcaaaca ctgccagtag ctcatggtgc tgagccgatc ggcgagcacg
     6421 agcgcttcgc cgcgacgctc gacgtgggtg cggggaccgg ggggctcgcg cagtacggcg
     6481 ccgcattcgg ggcactcgag acaggcgagc ggaacgagtg caccgcagtg gtcgcagcgt
     6541 tgcagcggca acggttgttc gcctttctcg cgccccttga gcgtccagtt ctgcggcgcg
     6601 tcggcggggc cgaaacggaa cgtgttgctc gcgtgatcga ggatgaacgc cttggctttg
     6661 cccggtgctg ggcggagggc tcgctcaatc tgctgcaaat gaagcgcaag gctcttcgtc
     6721 ggccgcagca ggatcaccgc caccactccc ggcacgtcga ggccctcgga gatgatcccg
     6781 cagttgctga ctatttgtag ttcgccgctg ccgagcgcgg cgatgatgct acggcggagc
     6841 tcggtcggcg tgttgccatc gagatgtgcg gcgcggtagc cgcgagcacg aaaggcctcg
     6901 gtcactagct cgctatgttg gatgtcgacg caatacacga tcgccgcccg gccggcgcag
     6961 agccgggtat attcgtcgac cgcggtgctg atgatgacac ccttcgacat ccgcgcggcg
     7021 agctcgtcgg tggcgtaatc accagcacga gtacggatgc cggagaggtc ggggctgcgc
     7081 gcaggcgcga aggtggcgaa cagcgacaga tagccggcgg cgatgagctc gctcacgctc
     7141 ggaccgatcg ccagcgcgtc gaagatgtcg tcaagcccct tgccgtcgag gcgctgtggg
     7201 gtggcggtga cacccaggac tttcgcttgc gggaaggcgg cgaggatacg ccgccacgtg
     7261 ttagcagccg catgatgact ctcatcgatg acgaaaagat cgggcggcat aagatgtttg
     7321 agccgccgca ccagggtctg cacgctcgca acttgtaccg gatgctcgag cgccgccaga
     7381 tcgcagccgg cgacgaccag cgcatgcgcc actccgaggg catcgagtgc ccgcgtgatc
     7441 tgctgtatga tttcgtcacg gtggccgaga atgcagacac gattgccgcg ccggacagca
     7501 ccagcgacga cctcgctgaa gattacggtt ttgccgcaac cgcacggacc ctgataacac
     7561 acacgccgaa caccgctgcg gaattgtgcg cgcagccgcg ccagatcgtc gacctgatag
     7621 ggacgcagca ccagcggcac ggccggtgtt gcgggcgaca acaaatcggg cacgagctcg
     7681 atgtcgctca tgcgccgcct ccaaaatggt cgatttcatt tccccaagcg gccggccgcc
     7741 gttcgcaggc gcttacggac cgcgtgacga tggtcatgca ggctccgagg ccactttgca
     7801 gcagcgcctc acaggcgacg cgaccggcgc cgatgagtac gactccggtc ccaggttctt
     7861 tgccgatcga gccgtcgggg cggatgaact ttgtcttgcc gctgggaaag agcaacaatt
     7921 gcgcgttagg cacgacaacc tcgtgaaacc aatcggccga ggtcctcgcc gcacacagcg
     7981 cgatgccgtt gccgtgcgcg aaaaacttcc gcagccaagg cacctgcccg cggcgaccgc
     8041 cgaatggcgg gttgagccaa accaggccgt gccacggctg ccgtagacca tcatcggcga
     8101 tcgtgtagat cttccgcgcg gggacgatgc aatacggatt gcttcggcct gggtgcgccg
     8161 cgtcgaggtc gaactcctcg atgccgccgc gcctaattcc atcgaaggtg gattgcggcg
     8221 tgaaccattc gagcggctcg atcggcaccg cgagctcgtg ctcagccatg tgcgccacca
     8281 tctggtaatt tcaggtcgca gccaaagaaa ttacgtccga gctcgagggc ggcgtgcagc
     8341 accacgaatg agccggcgca gggatcgatg atgacgtcgt ctggctgcgt ggttgcggcg
     8401 atcaggcgct tgatgagtcc gatcggcttg gtgtgcgggt aaagttttcg ggggtagcgt
     8461 ttcaggtcaa tcttctcgac ccaacggcac caaatcccgc ggtctcgcca cgtcgcgcgc
     8521 gccctcttcg gcggcttctg caaggccaac aaataatcgc cgcggttacg acaacggtct
     8581 ccttgcccag ggcgcaagtt gtcccaagcg atcaaatcga cacgctcgag gagttgttga
     8641 gaaatacgga gatgatgtcc ctcgcacaga caaaacttgt ccatccagcg catcaaatag
     8701 ccgctcggct taaggatgcg tgcgctctcg atgcagcatg cgtcgatata atcctcgctc
     8761 atcgccggca gcttggcgcg cccgcgctgg cgttcgcctt cgttgccgta cttcttcttg
     8821 tccatgatcg cgcgatgctg cggatcgaag aacacaagcg gcgcgtagcc gtccggcagc
     8881 aaacgcaaca aaaccagtgc gtcgctttgc tgggcaacat ttaatcgcaa ggcagcaaac
     8941 ggcgcgggag caacgcaagc tgcatcggga gtctcgtcta ggccgtgccc atatccgctc
     9001 gtacgctgca tcaccactca ctccacccga acaagaaggg ggcccgctgc gcgagccacc
     9061 ctcacgcttg ttgttcttca aaacggagct tcttcctctt ccaccgcgtc tacggcgcgt
     9121 gccggcggct ttcgcggtgg tggcggctcg ggcaatggac cgttgccagc cgtcggtggt
     9181 ggagtagcgc agcgtacgcg aatgccgccc tttttttcgc cctgatactc gacatcagga
     9241 tcgtaataga gcgtgatcca gttcccacgc cacttgtcac cgtcatcgcc aagcaagcgc
     9301 gcaagagcac gtgcatttgt cggattaaga actaggcaac gatcttcccc gacgaagtgc
     9361 acgagatcgc gcgtttcttt acccatcaga acgtcccact ccacgtagct aatctgcagg
     9421 gtcaggttgc cggctttgac gtcctgcgca gtcatcaagc tcgagccgta gcgttcgctg
     9481 atactcgtca tctcaaacct cctgttggtt gacgggttac gtttgcttct cactgaaact
     9541 tgccagttcg tatcggcatc acgatcgcga acgctgctgc gtcgtccgaa tcagtgatgc
     9601 ggatcgctgg gtttgagggc ccgttgacat cgagcgtgac cttcttgccg ccgaaggcct
     9661 cgagcacgtc ggtgagataa gaaacgcgcg cggcgaaacg cccatcgccg gtaatttcaa
     9721 cgccctcgat aatgtcgtcg gttttcgtcg gtgccagtca ttgagacatg caacgcctca
     9781 ttcgaccacg cgagccccgc ggtacgcatc acgtgtttca ttttttggtc gaacagcgcg
     9841 tcaatacggg cgagtgcctg acgcaagttg tcggtatcga cggtgacggt gttgccagat
     9901 gccggaggaa tgatgcgggc gtagtccgga aacgtaccgt cgatgagctt cgcggtgagc
     9961 gccaggccgg gcagtaaaat ctcgaccaac ctatcggaga gtcgcaccga aactgtttcg
    10021 ggcgccggtt tcctcttcag cagcttgatg aggatcgcga cgatctttgc tgggacgatg
    10081 atgccggaca tctcatcggc acctgcaggc ataggcagat cgaaccagca gagccggtgc
    10141 ccatcagtgg cgacgccacg caaagtcttg tcggcggtgt gaaggtaaag accgtttaga
    10201 taatagcgca tctcctcggt cgagatcgcg tgctcgcaga cctccagcat ccgccgcagc
    10261 tggtcagcgt caagatcgaa actagtcgcg ggatcagcga gctcgctcga ttgcggcagt
    10321 atgtcgatcg gcaagcccgc gatcgtaaag cgcgacctcc cggcacgaac gatgatgccg
    10381 ttaccgctgc tctcgaccgt gacggtgcgt ttggggtcca atccgccgag cacaccggcg
    10441 agggcggtgc aacgcgccga tacctccccg ggctctacga tcgttgccgt gacattggtc
    10501 gtgatgcggc ggtcgaggct atcgacgacg aagacgacgc tgtcccgcac gacgatacgc
    10561 gccatgctca gcgccgcgac gttggacttg tcgtccagcg ccagcgccgc ggcaccgagc
    10621 gcggcagaca aattgccagc ggtcgcctcg aatttcacag atatcaccta tcagtgatcg
    10681 tgatcgcgtc ggcgtggcaa cgctggcaag tcagagtata ttcgccgtcg ccgatgtcgc
    10741 gcatggcgct cgcgcgcagc tgtgctcggc agcgcgggca acgcaaatcg ccgaagaggc
    10801 gaatggcaca gccgccgatg aacgcggtgt cggtcgtggt ggtgggtgtg tcggttttga
    10861 atttgggcat cgtggtcatg gggttttctc ctggggttgt tagcgagcgg gcggctcgcg
    10921 cgcgctgacg agcataacgg ccagagcacg tgcggcagcg gttccccgcc gactgatctc
    10981 agtttcgatc gcttctgaac gtattcgagt atcggtgagg gcggcgcgta gctcgtcact
    11041 actcatctgc tgcagttccg gattggcgtc cggcggcggc ggcggcgctg gcgcgtcgtg
    11101 aatacgcttc acctcgaaat gtctgctgcg ctcagcgaga ggaacgccaa gcgctgcttg
    11161 tgtgcggtcg agcgcttcga tcgtggcgcg gtatgcggtc cgcgtcgctt cgttcatcac
    11221 caagtcgggc gccagcacaa gacgttcgag gctgccgcgc ataacgtaca gtgttgctgg
    11281 cgcttgcttc gcaaattcga gcatcagctt tgcggccgct tccaattcgc ccaacgtgtg
    11341 tagccaatac tggtcttcgt taaattcgta atcggcgaac accaagtttg cgatcgtggg
    11401 atgtccgagc aactgcacaa ggcgcttgat caatcgctgg agtgcatcct gatagtcgtc
    11461 gatctgaacc gacacctccg cggggcgtgg caacgctgcg acgatcttgc cgccggcaat
    11521 ctgctcccat tcccaacgct gcgccgcttg ccactcggcg gcggtgacgg tgcggccgac
    11581 caagtggctg aggtagtcgg gcatctcgtc atcgcagaca ggagcgtgtt tgcatccatc
    11641 agcatcgacc ggaatgctct cccacatccg tttgtacgca tcgaggatct gctgatagcg
    11701 cgtggtgagc gtccgtacgt tgctgtcatc atgcatgacg tacctcctcg ctcagcaccg
    11761 aagtgacgaa gtccaccggc atgccgctca gcatcacgat gcgctcgagc ggccaaccgt
    11821 cagccgcccg cgcgtgaatg aatttgatcc gtttggcgtg gcagtcgggc ccgatgccgt
    11881 gctcgagcga gatcggatcg gtcagttctt tgaagcaaat acagcaatgc ccacagagcc
    11941 gcgctgcaag ctgcatctgg gcatcagggg caccgatgaa atccagcacc cgcgcccgcg
    12001 ctagttcgag cgcgcgttgt cgcagccgct gtcggtcacg atgagccttt gcttttgtca
    12061 gatcctcggc gtcgatcgcc tcgatctgac gcgcgatctc agcgagacgt gtccccagct
    12121 gtcggtcgtt atagattgtt ggcgcgcgtt ctcgctcgcc gattaaacgt tcccactccc
    12181 gctgtttcgg actgaagtaa gggaagaaat actcacgcgg tccaaatcgc caagtaccgg
    12241 attcctctcg ttgtgcttca aatagaatat ttcccgtcga tccccgtgtg ccgccggcat
    12301 gaagctggaa gccgcggcag ctaagacctg ttagcggaag attgctgacg tgctctcgca
    12361 gccaccgacc tgagcgtttc ggtgtcgcga acgtcgccca gaaccgcgca atccatttgt
    12421 tatcatccgt gagatagcca agctcgtagc aaatattggc cagttcttct tcaaggacca
    12481 gcggtggcgc ccacgaggga cggcgctcag catcttcagt gatgctcacc tcggcttctg
    12541 cgcgctgccg gagccgctcg aaggcttcga aaactgtcgc gtcctggatt ggtggcggag
    12601 tgtgatcctt cagcggcgcg attgcggcgc gctttgcctt gtaggcgtcc ctgatgactt
    12661 ccgccgtgtg ttgcgcgaat gccctttctt cctcacgccg ccgttccagg tgctgctgat
    12721 aggcagcacg ctcggcagcg ttgaaccgat cgaaataaag cgtgtcgtta cttcgatcgg
    12781 tatcaatcct gatgtagcgc ttgtcgtccc aggagatttt agcctcacca cagccggctg
    12841 cgagcaacgc gtcctccacc tgcacaaccg acgggccctc atcggtccac gtgatgctgc
    12901 agcctgcaga caaagcgacc gcgaatttcg tgccggggaa cgcggttctg agcgcggcgc
    12961 ggatttttcg ggaggcttcg gctgtggtaa tcatgtgttc actccctgct gatgaagaca
    13021 gcgccagctg ttgaggtcta tgacaccgac tccaattcgc gtgtttcccg ccggcaaact
    13081 tcacagctgc agagattcga acgctgacct ctccaaaatt ctttctcgtg ctgacctagg
    13141 cgatctgaat ggttgagcgt ctgttcgatc tcctgtcgga cgcggcgtgg gtcgcaatcc
    13201 caatgcgatg ttgggtcgca accaacaatt tcctcagcca aattctcggc agcggtgcgc
    13261 aagttctcca gcaagtccca cgcggcaata cacttgcgtt cgcagaatgc atgcgtcaga
    13321 tcgtcgacga acggcttagt ggctttaatc tcagcgatga ggtgctcgat cacgacctca
    13381 gcggcagtct ttccgtattt gaaggtgtca atgggcatga tgtttctcct gaaaaaagtt
    13441 gtgtgacggt cgatagtgtt gtcgcctccg aaagggtgcc gcgcgtgcgc gacgcccgtt
    13501 tttgctcgat acgctctaga gcgccctaac cagcgcggcc cagatcacct cggagcctgc
    13561 gatcttgaac gaggtcgcca actcgtcagg gttcatcgtc ttcaacgcct ccgcgaactg
    13621 aatcgcagag tcggtggagc cgtggctcgt accgttgccc gtgccgttgc cttcgctgtt
    13681 gatgacatca gcagtgatgc cagccagctg ttggccaccg ttcgcgatgg cccactcgcg
    13741 gatgccgacg ttgactgctc tcgccgacac gccgtacatc gaacacacgt ctttcgtgcg
    13801 aaagcctgtg ggctggagta aacccagccg cgtatcgacg gccagagcta cccgcttttc
    13861 ctcaggaaga tgccgcaggg cgagcccgcg gaggttaaag gtcgcgttga cggagcgacc
    13921 gccgttcgct agatacagca ttgtctggca cctccatgtg tccagatttc acacattaag
    13981 ctttgacttc cctaggcggc gctgcgttcg acagcgtcgc ctctctcgct cattgttgcc
    14041 gcttgttccg agcgagcccc tcgggcgact gtcctcctcc tattccgccg cctccggtga
    14101 aggtgacggc ttcgcgcgca acgtggcttt ttctctcgcc ttgcgccaat tgctggcagc
    14161 ctcatgcgag atcagtaccc tcgcgccaat tcgcatctcg cgcggtccga gcccacggtt
    14221 cctaagcttg tggtagaagc tttcgctcaa gtcgtgagcg acacagaaac ttgaaatcgt
    14281 gaaagcgccg gactgcaggc ccctgaagcg ggcacgctga gtcgtcttcg tgcgcgcggg
    14341 ccgcttcgcc atcgcctcgg ctttgtcgga aaagcgggcg ctcggcggtg cccgggccgc
    14401 gtggacatgt tcgcagtcgt ggtcatgaga aggaaccgca acagcaagac cggcttcgag
    14461 ccgatactta gtgttggctg cggcgagttt cggactcagg gccggtgctg cagtcctggc
    14521 tttgagagcg gctgccttct tagataacgc cgtctgtgca ggcgctgctt tctgcttgat
    14581 ggttttgcgg cgcgccatcg aggcctcgat gcttgttcat cgggcaccat atgactcgct
    14641 gaggttttga cgaagaatag acgaggataa tcgaggacaa ttagactcag gcgacttagt
    14701 cggccaaatc tcggagcaat ttgttaatag cgctcggatc cggcatgtag cccatttctg
    14761 cctcgcaaag ctcccctagc gtcccagagc tcggaaacgc tccatgcttc tttttgtggt
    14821 cgtaggcgag ccacgcgatc accagtttcc aaccctgatg cttctttggc ccaggtttac
    14881 gccgttgttg tggtgacgtc ttcgcggcgg cggcaaactt ctcatcgatc agctgcttaa
    14941 cagcttcgac atcgatcccg tctttcgggg cgggcaaccc cgctatggcg gcggtgatct
    15001 tctcagcaat cagctcggtg ttggactcct tcaacaaggc ggttatcccg gcggccgaga
    15061 cctgccactt acgagccgac tggtcggcct ccatcgcccg ggcccgctcg taccccccga
    15121 cgcatgcctg gtgatcgccg ttccccacca tatctgtcgc tgcccagagt ttaagccgct
    15181 cgcagccgtc gtcatccgtc cagacatcca cgctcaaaga gagttgaaac aaatgaggct
    15241 caagcagcgt cccgtcacac caaatggcac agtctccatc cagccacgcc ttatagagtt
    15301 ctcgagccgc agggtgcggt cggtaatcgg acaggagttg ttggaagccc tcatcaaacg
    15361 agattcgctc gctttggggt gatctcggca gctgcctgct aggcgacggg tgggtattag
    15421 aagcggaggg gggaagttgc ggtctgcgcc gccgtcgact atttttgccc atagcgtggg
    15481 tccactttgg cggtgggaac atgctttcga ggacccggca ggattaacca ctcggccggg
    15541 tcctctcatt tcaatccatc gcatcatcag ccgcaacctt tcatgccttg ccgtgcagcg
    15601 ggcggacgtt ccccggcttg atgccgtaga tcggcgccgc cttgcggatg gcgtcagcag
    15661 cgtaactcgg cgcaaggtgg ctgtagtgct tctcgaccat acgcgtgtcg gtgtgtccga
    15721 gtgcctgcgc caccaccaga agcggggcgc cgttcatcac ggcgtggctg gcgaaggtat
    15781 gtctggtgac gtgaaaattg accggcggac taataccggc atgccgcgag gcctcctcga
    15841 ttgggatatc ctggtgcgac ttctcccatg gcgtcccgtc ggcgtgggtg aagatcagat
    15901 ccttgccggg gcgtcccgcg accgcggcgc ggaagaacgc tagcgcctcg tcgctgagat
    15961 gcgcgtggta ggcgcgttcg ctaccgtcgc ccttgcgggt gcgcagccgc agcgtcccgg
    16021 cgtcggtatt gagatccgac accgtaagct gggccagctg gccgtacctc gcgccggtca
    16081 ataacgcccc ctgcaaaagt tggcggaagt cggggctcgc ggcgttgatc aggcgcttgg
    16141 cttcggcgac cgacaggtag cgcaccctcg ccttatcgac gccgcgaaac ggacgcagcc
    16201 ggcgccaggc gtggtcagag ctcacgtgac cggcgcgaaa agccacattg agggcggcga
    16261 ccagcgtggt gcggatccgg ttggcggtag cccggcgggc gcgatcggcg tcggtttcat
    16321 cgaggtcccg ccggccctta cgccgcgccg gcttgcggac gagaccatcg aaccaggcct
    16381 taagctgctc gtaggtgagc ttggtgagct cgacgtcgcc gagtaccggt aggatgaggg
    16441 catcgatgtt gtagcgcgtg cggctgagcg ccgccggcgg gcggccctcg gcttccaggc
    16501 ttttgagata ggcggcggtc gtgtctgcga tcgtgccgat gccgccaccg gccagctcaa
    16561 ccatgagctt gcgagcggcc ttctgcgcct gcgagtagga cagaatcacc ctgccatcgg
    16621 catcggaaaa gtcgtcggcg aggccgatcg gatgatagcg atagctgtgg ttaccgacgt
    16681 agcagcgcgc tagccatttg ccggtcccac tctttggctt gcggtagccg agatgaagta
    16741 atccgggctc gagtgccttg tagtatggct ccccgcgcgg cgctaaccgt tcgcgggcag
    16801 tgcgactctc gagggcagca tctctaacag tgcgagccat agctaatctc tcgtacgttg
    16861 aatttcttgg aatgggtggc gctgcttgtt gtaggtcgtg ttgaagcggg gaaattcgtc
    16921 gcgaatggcc gcttcctcgg ccgttgcctt tccagccata cgctcgtacg caactcgatc
    16981 cgtacgggat acccacggga tatgcatgtg tggagcccta tagagcacca acgagctcaa
    17041 tgcagaaagt gcccgtctta tcggcggttt acaagagccc tatagagccc tataacgcgc
    17101 ccgcgcccgg gacagggcgg ctggcagatg ttccatacct ggcatgccgg cgcggtttgt
    17161 atcgatccgt cgtcctatac cgcgatccgc gccaacggcg acaaggcgtg gttcggctgg
    17221 cccaccagtg cgaaggtcga agccgagat
//
LOCUS       DATLJT010000731         4135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2113045,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000731 DATLJT010000000
VERSION     DATLJT010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113045"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VM910_16770"
     CDS             <1..306
                     /locus_tag="VM910_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64216.1"
                     /translation="IAAALLAASADAAEVRTKPAAAGAAVAAAPAAEAIQDCSAFYDD
                     YARRGWGWGSGPGTSVGFGTFEGALPRYPVNEFPNWYGDCVNWGNYSATGTAFGSGR"
     gene            385..2067
                     /locus_tag="VM910_16775"
     CDS             385..2067
                     /locus_tag="VM910_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVI64217.1"
                     /translation="MVWSQIYNPLGNAWLSTLVAAIPIVVLLGALGIFRIKAHLAAIY
                     GLIASLLVATIVYGMPVGMAGETAVFGAAFGLMPIGWIILNVIFLYQLTLEKGYFKVM
                     QDSIAGVSQDRRLQLLLIAFCFGAFFEGASGFGTPVAVTGAILIGLGFSPLAASGLSL
                     IANTAPVAYGALGTPIIALAAVTGLNLYDLSAMVGRQLPFFSLIVPFWLIWAFAGWRG
                     MLEIWPATLVAGVSFAIPQYLISNYHGPWLVDVLSSVVSMGCLIAFLNYWRPQKVWTT
                     TIPRGRQAALAANDGAQDPGATAAAPTVPPRAEVVKAWIPWLILSVAVILWGTPQFKT
                     LLDGISVVRIPVTGLHNLIERMPPVVLKPTAEAAIYLFNWLSATGSGILVAAVVAGLV
                     MGFSPLGLVRSYWNTIKLVRYSLLTIACMLAIGFITRYSGIDATLGLAFANTGWLYPL
                     FGTMLGWLGVALTGSDTSSNVLFGSLQRITAEQLGLSPTLMSAANSSGGVMGKMIDAQ
                     SIVVASTATRWYGHEGDILRYVFLHSLALAVLVGLLVSLQAYVWPFTALVVR"
     gene            2158..2901
                     /locus_tag="VM910_16780"
     CDS             2158..2901
                     /locus_tag="VM910_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVI64218.1"
                     /translation="MRVGLFIPCYVDAFFPAVGIATLELLERLGLEVVYPLDQTCCGQ
                     MMANSGSQKDAAGAEALFVKNFKDFDHIVTPSGSCAYHVREHLDAIPQTEEAQKVRSN
                     TYELVEFLHDVLKVEAFPWAEFPHKVGLHNSCGTLRKLRTAKASEIDEPFFSKPLDLL
                     SKVRGIEFVTPSRPDECCGFGGTFSIFEEPVSAKMGYDKVADHHAAGAEYIVSADMSC
                     LMHQKGCAERVGLDIRFIHIAQILNGDRA"
     gene            2898..>4135
                     /locus_tag="VM910_16785"
     CDS             2898..>4135
                     /locus_tag="VM910_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HVI64219.1"
                     /translation="MSRIDHAEAASEFIAARAQQEFHDRRLWDLRLKRDREAHGIPEW
                     EALRTLASQIKEHALARLSDYLEEFETRAKENGAHVHWARDATEHNQIVYEILSVRRV
                     KTLVKSKSMLTEECDMRPFLERRDIEVIETDLGERIQQLDDEPPSHIVVPAVHKLRGD
                     VSEVFARTIGAEPGNDDVHYLAETQRQTTRPYFLRAEAGMTGANFAVAETGSFVVCTN
                     EGNADLSANVPKLHIASIGIEKIIPKTEHLGVFIRLLSRSALGSPITQYTSHFRAPRD
                     GGELHIVLVDNGRSQRLGMHDFWYSLKCIRCGACMNTCPVYRRSGGLSYGATYSGPIG
                     IIIDPTFNIRKYSNLPFASTLNASCTNVCPVKINIHEQIYKWRQIIAERNQLPFVKKE
                     MMRVAGKVLADPRLYRAAISA"
BASE COUNT          748 a         1314 c         1229 g          844 t
ORIGIN      
        1 atcgcagccg ctcttctcgc ggcgagcgcg gacgccgcgg aggtccgcac caagcccgcg
       61 gccgctggtg ccgccgttgc tgctgctcca gcggccgaag ctatccagga ttgcagcgcg
      121 ttctatgacg attacgcccg ccgtggttgg ggctggggtt cgggaccggg aaccagcgtc
      181 ggcttcggca cgttcgaagg cgcgcttccc cgctacccgg tcaacgagtt ccccaactgg
      241 tatggcgatt gcgtcaactg gggcaattac agcgcgacgg gtaccgcgtt cggcagcggt
      301 cgttgacgca attggcagcg ctcgttgaat gagtgtcgcg gttgcggaga gatgttgact
      361 cggcagtgac ctgacgaggc agcgatggtc tggtcgcaaa tctacaatcc tttgggcaat
      421 gcttggctgt cgacccttgt cgccgccatc cccatcgtcg tcctactcgg cgcgctcggc
      481 attttccgta tcaaggcgca cctggctgcg atctacggtc tgatcgcctc gctgctggtc
      541 gcaaccattg tctatgggat gccggtcggc atggcgggag agaccgccgt ctttggtgct
      601 gctttcggct tgatgcccat cggctggatc attctcaacg tcatcttcct ctaccagctc
      661 acgttggaaa aaggctactt caaggtgatg caggacagca tcgcgggcgt gagccaggac
      721 cgtcgactgc aactcctgct gatcgccttc tgcttcggcg ccttcttcga gggcgcgtcg
      781 gggttcggaa cgccggtggc cgtgaccggc gccatcctga ttggcctcgg gttttcaccg
      841 ctcgccgcct ccggattgtc gttgatcgcc aataccgcgc ccgtcgctta tggcgcgctg
      901 ggcacgccga tcatcgcact cgcggcggtg accggcctga acctctacga cctcagcgcg
      961 atggttggcc ggcaacttcc gttcttctcg cttatcgtac cgttctggct gatctgggca
     1021 ttcgccggct ggcgcggcat gctcgagatc tggccagcca cgctcgtggc cggcgtcagc
     1081 tttgcgatcc cgcaatatct gatctcgaat tatcatggac cctggctcgt cgacgttctc
     1141 tcctcggtcg tctcgatggg ttgtctgatt gccttcctca attattggcg tccgcaaaag
     1201 gtttggacga cgacgatccc gcgcggccgg caggccgctc tggcagccaa tgacggagcg
     1261 caggatcccg gagcgaccgc cgccgctccg acggtgccgc ctcgagccga ggtggtcaaa
     1321 gcgtggatcc cctggctgat cctcagcgtc gccgtgattc tgtggggcac gccgcagttc
     1381 aagactttgc tcgatggcat ctcggtcgtg cgcattccag tcacgggttt gcacaacttg
     1441 atcgagcgga tgcctcccgt cgtcctcaag ccaacggctg aagccgccat ctatctattc
     1501 aattggctct cggccaccgg ctcgggcatc ctcgtggccg cggttgttgc cggcctggtc
     1561 atgggctttt cgcccttggg cctcgtccgc agctattgga atacgatcaa gctcgtacgg
     1621 tactcgctgt tgacgatcgc gtgcatgctc gcgatcgggt tcattacccg atattccggc
     1681 atcgacgcga cgctcgggct cgccttcgcc aatacgggtt ggctctatcc gctgttcggc
     1741 accatgctgg gctggctcgg cgttgcactc accggctccg acacgtcctc gaacgtgttg
     1801 ttcggcagcc tccaacgcat caccgccgaa cagctggggc tcagcccaac cttgatgtct
     1861 gcggcgaata gctcgggcgg tgtcatgggc aaaatgatcg acgcccagag catcgtcgtg
     1921 gccagcacgg cgacgcgatg gtacggccat gaaggcgaca tcctgcgcta cgtgttcttg
     1981 cactcactcg ccctggccgt cctggtcggc ctcctcgtct cgcttcaggc gtatgtctgg
     2041 ccattcaccg ccctggtggt gcgctgacgc aatgggcatg gacgctgtgt caccggccga
     2101 tcaaactcgg cccggcgtca cctcggacaa accgttctcg cgtgaagagc atcgatcatg
     2161 agagtgggat tgtttattcc ctgttatgtc gacgccttct ttccggcggt cggcatcgcc
     2221 acgctggagc tgctggaacg gctggggctc gaggtcgtct atcccctcga ccaaacctgc
     2281 tgcggccaga tgatggcgaa cagcgggagc cagaaggacg cggccggggc ggaggcgttg
     2341 ttcgtcaaga acttcaagga ctttgatcac atcgtcacac cgtcggggag ctgcgcctac
     2401 cacgtgcggg aacatttgga tgcgatcccg cagaccgagg aggcgcagaa ggtgcgaagc
     2461 aacacttacg agctggtcga attcctgcat gacgtgctca aagtcgaggc cttcccctgg
     2521 gccgaatttc cgcacaaggt cggccttcac aattcttgcg gcacgcttcg caaacttcgc
     2581 accgcaaagg catcagaaat cgacgaaccg tttttctcta agccactcga tctgctcagc
     2641 aaggtgcgcg gcatcgaatt cgtgacgccg agccggccag acgaatgctg cggcttcggc
     2701 ggcacctttt ccattttcga ggagccggtt tccgccaaga tgggttacga caaggttgcc
     2761 gatcatcacg ccgctggcgc cgaatacatc gtttctgctg acatgtcgtg cctcatgcac
     2821 caaaagggct gcgccgagcg agtcggtctc gacatccgct tcatccacat cgcgcaaatc
     2881 ctcaacggag atcgcgcatg agccggatag atcatgccga ggcagcctcc gaattcattg
     2941 ccgctagggc gcagcaggaa ttccacgacc ggcggctttg ggatctcagg ctcaagcgcg
     3001 accgcgaggc acatggcatt ccggaatggg aggcgctgcg caccctcgca tctcagatca
     3061 aagagcacgc gcttgcgcgt ctctccgact atctagagga gtttgagaca cgcgcaaagg
     3121 agaacggcgc ccatgtgcac tgggcccgcg acgccactga gcacaaccag atcgtctacg
     3181 agattctttc ggtgcggcgc gtcaagacgc tcgtcaagag caaatcgatg ctgaccgagg
     3241 aatgcgacat gcgccccttc ctcgaacggc gcgacatcga ggtgatcgag accgatctcg
     3301 gcgagcgcat ccagcagctc gacgacgagc cgcccagcca catcgtcgtg ccggcagtgc
     3361 ataagctccg tggcgacgtc tcagaagtct ttgcgcgaac gatcggggcg gagccgggca
     3421 acgatgatgt ccactatctt gcggagacgc agcggcagac gacgcgacca tattttctgc
     3481 gtgccgaggc cggcatgacc ggcgcgaatt tcgcggttgc ggagaccggc agcttcgtgg
     3541 tctgcaccaa cgagggcaat gccgacctca gcgccaacgt accgaaactc cacatcgcat
     3601 ccatcggcat cgagaagatc atcccgaaga ccgaacacct gggtgtgttc atccgcctgt
     3661 tgtcccgcag cgccctaggc tccccgatca cacagtacac atcgcacttc cgagcaccac
     3721 gcgatggcgg cgaactccac atcgtgctcg tcgacaacgg acgctcgcag cgcctcggca
     3781 tgcacgattt ttggtactcg ctgaaatgca tccggtgtgg agcgtgcatg aatacctgcc
     3841 cggtctaccg ccgaagcgga gggctgagtt atggcgcgac ttactcgggt ccgattggga
     3901 tcatcatcga tcccactttc aatatccgca aatacagcaa cctcccgttc gcttccacac
     3961 tgaatgcgag ctgcaccaac gtctgcccgg tcaagatcaa cattcacgag cagatctata
     4021 aatggcggca gatcattgcc gagcgcaacc agcttccgtt cgtgaaaaaa gaaatgatgc
     4081 gcgtcgccgg caaggtgctg gccgacccga ggctgtatcg cgcggccatc tcggc
//
LOCUS       DATLJT010000732         4617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1194521,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000732 DATLJT010000000
VERSION     DATLJT010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194521"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..3122
                     /gene="ileS"
                     /locus_tag="VM910_16790"
     CDS             123..3122
                     /gene="ileS"
                     /locus_tag="VM910_16790"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HVI64220.1"
                     /translation="MSEKPQKSDARDYSKTLFLPQTEFPMRAGLPQREPEILKRWNEI
                     GLYDRLRQAAKGRTKFVLHDGPPYANGNIHIGTALNKILKDLVVKSQQMLGMDSPYVP
                     GWDCHGLPIEWKVEEEHYRSKGKQKPDFSDPAAMVAFRRECRAYATHWLDVQREEFKR
                     LGVVGDWEHPYATMSFPAEAQIARELMKFAANGTLYRGSKPVMWSVVEKTALAEAEVE
                     YEDYTSDQVWVKFPVTSPAHGALANASVVIWTTTPWTLPGNRAISFSPKIAYGLYKVT
                     DAPADNWAKTGDVLILAEVLADSVFKQARVTAYEKIRDIPADTLDAMECAHPLKGLAG
                     GYGFTVPLLAGDHVTDDTGTGFVHTAPGHGREDFDVWTANARELEARGIATAIPYTVD
                     ENGAFTDHAPGFTGKRVINDKGEKGNANDAVIKALADAGMLIARGKLKHQYPHSWRSK
                     KPVIFRNTPQWFIAMDKDIQDGGKARPGDTLRARALRAISVTQWVPPSGQNRITGMIE
                     ARPDWVISRQRAWGVPIAVFVREKGDGSAEILQDDAVDTRIADAFEKEGADAWYMEGA
                     RERFLGSRANEDWNKVDDILDVWFDSGSTHAFVLEDPVHFPGLAGIRRKADGGSDTVM
                     YLEGSDQHRGWFHSSLLESCGTRGRAPYDVVLTHGFTQDEHGRKMSKSLGNTVEPQAV
                     IKQSGADILRLWVAACDYTDDQRIGPEILKNTVETYRKLRNTIRWMLGTLHHFKPGEE
                     VDVKEMPELERYMLHLLAEQAGIVRKAYVDYDYKTVVATLSAFMNSELSAFYFDVRKD
                     ALYCDPPSSVTRKAALTAIDVICDAILKWLAPVLSFTCDEAWAMYHPAGSPSVHLLTF
                     PEHLEAFRDDALAKKWETVRNVRSVVTGALELARAAKRIGSSLEASPIIYVADRKMLA
                     TLFDVDLAEVCITSNYEVQEDEAPAGAFRLDPVPGVAVVVEEAQGRKCARSWKILPTV
                     GDDPEYPDVSPRDAQALREWKALGVSA"
     gene            3278..3775
                     /gene="lspA"
                     /locus_tag="VM910_16795"
     CDS             3278..3775
                     /gene="lspA"
                     /locus_tag="VM910_16795"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVI64221.1"
                     /translation="MKSPLLPGVLAAVATLVVDQASKLWLLHVFDIGHRGTVKVTPFF
                     DLVLAWNIGISFGWFQNDNPVAQIALMAIKALAVVALAIWMARSRTVLATVALGLIIG
                     GAIGNAIDRIAYGAVIDFALFHVQIGQNTFNWYVFNLADVAIVAGVMALLYDSFIGVP
                     AAKAP"
     gene            3817..4545
                     /locus_tag="VM910_16800"
     CDS             3817..4545
                     /locus_tag="VM910_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64222.1"
                     /translation="MRKTEAKDWMGQHSSPTLTRAARLSAVVLGVGLLVASGAARAQE
                     ADDEDKTFEEKIIEGIMKGIGGTNMDNTGIDYRERSPLVVPPKLDLPQPAASSTDVKA
                     PNWPKDPDEARRKAAIAARKKANKDPMEASRILSPSELNAGRTAAGTSGGTKGSDSAV
                     PGGDPGRNALLTPSELGFEGRFSNLFGGNKTEIKEFKAEPPRDSLTQPPPGYQTPSSN
                     FAYGTGPKESLNKEYNPAAGKYGE"
BASE COUNT          903 a         1470 c         1451 g          793 t
ORIGIN      
        1 cgcaccatag ggattagcgg cccggcttcc gcctgagcct ccggctcggc gcaagaccgg
       61 gactgtcctc gtttcacccg cgatcgatcg cgttttccgc gcctctccga gccagattgg
      121 ttatgtccga aaagccgcaa aagtccgacg ccagagatta ttccaaaacc ctcttcctgc
      181 cgcagacgga gtttccgatg cgcgcaggcc tgccgcagcg cgagccggaa attctcaagc
      241 gctggaacga gatcggcctc tacgacaggc tgcgccaggc cgctaagggc cggaccaaat
      301 tcgtgctgca tgacggcccg ccttacgcca atggcaacat ccatatcggc acagccttga
      361 acaagatcct gaaggatctc gtcgtcaaga gccagcagat gctgggaatg gattcgccgt
      421 atgtgccggg ctgggactgc cacggcctgc cgatcgaatg gaaggtcgag gaggaacact
      481 atcgctccaa aggcaagcaa aaacccgact tcagcgatcc cgcggcgatg gtggcattcc
      541 gaagggaatg tcgcgcctac gccacgcact ggctcgacgt gcagcgcgag gagttcaagc
      601 ggcttggcgt ggtcggcgac tgggaacatc cctacgccac catgagtttt cccgcggaag
      661 cgcagatcgc gcgcgagctg atgaaatttg ccgccaacgg cacgctttat cgcggctcga
      721 aacccgtgat gtggagcgtg gtcgagaaga ccgcgctcgc cgaggccgag gtcgagtatg
      781 aggactacac ttcggatcag gtgtgggtga agtttccggt cacctcgcca gcgcacggcg
      841 cgttggcaaa cgccagcgtc gtgatctgga ccaccacgcc gtggaccctc ccgggcaacc
      901 gcgccatctc gttctcgccg aaaatcgctt acggccttta caaggtgacc gatgcgccgg
      961 ccgacaactg ggccaagacc ggcgacgttc tgatcctcgc cgaagtgctc gccgacagcg
     1021 tgttcaagca ggcccgcgtg acggcctatg agaagatccg cgacatcccc gccgacacgc
     1081 tcgacgccat ggaatgcgct catccgctga aaggacttgc cggcggttac gggttcacgg
     1141 tgccgctgct cgcaggcgat catgtcaccg acgacaccgg tacgggcttc gtgcataccg
     1201 cgcccggcca cggccgtgag gacttcgacg tctggacggc gaatgcgcgg gaattggaag
     1261 cgcgcggcat cgcaaccgcc atcccctata ccgtcgacga aaacggcgcc ttcaccgatc
     1321 acgcgcccgg cttcacggga aagcgcgtca tcaacgacaa gggcgaaaag ggcaatgcca
     1381 atgacgccgt gatcaaggcg ctggccgacg caggcatgct tatcgcccgc ggcaagctca
     1441 agcaccaata tccgcattcc tggcgctcga agaagccggt gatcttccgc aacacgccgc
     1501 aatggttcat tgcgatggac aaggatatcc aggacggcgg caaagcgaga cccggcgaca
     1561 cgctgcgcgc ccgcgcgctg cgcgcgattt cggtcaccca atgggtgccg ccgtcggggc
     1621 agaaccgcat caccggcatg atcgaggccc gccccgactg ggtgatctcg cgccagcgcg
     1681 cctggggcgt gccgatcgcg gtcttcgtgc gcgagaaggg cgacggctct gcagaaatcc
     1741 tgcaggacga cgccgtcgac acgcgcattg ccgacgcctt cgagaaggaa ggcgccgacg
     1801 cctggtatat ggaaggcgcg cgcgagcgtt ttctgggctc ccgcgccaac gaggactgga
     1861 acaaggtcga cgacatcctc gatgtctggt tcgattccgg ctcaacgcat gccttcgtgc
     1921 tggaagatcc cgtgcatttc cccggtcttg ccgggatcag gcgcaaggcc gatggcggca
     1981 gcgataccgt gatgtatctg gaaggcagcg accagcatcg cggctggttt cattcctcgc
     2041 tgctggagag ctgcggcacg cgcgggcgcg cgccttacga cgtggtcctg acccacggct
     2101 tcacccagga cgagcacggc cgcaagatgt cgaaatcgct cggcaacacc gtcgagccgc
     2161 aggcggtgat caagcagtcc ggcgccgaca tcctgagact ttgggtcgcg gcctgcgact
     2221 ataccgacga ccagcgcatc ggccccgaga tcctgaagaa cacggtggag acctatcgca
     2281 agctgcgcaa caccatccgc tggatgctcg gcacgctgca tcatttcaaa ccgggcgagg
     2341 aggtcgacgt caaggaaatg ccggagctcg agcgctacat gctgcacctg ctcgccgagc
     2401 aggccggtat cgtgcgcaag gcctatgtcg attacgacta caagacggtg gtcgcaacgc
     2461 tgtcggcgtt catgaacagc gagttgtcgg cgttctattt cgacgtgcgc aaggacgcgc
     2521 tctactgcga cccgccgtcc tcggtcacgc gcaaggccgc gctcaccgcg atcgacgtca
     2581 tctgcgacgc catcctgaag tggcttgcgc cggtcttgag tttcacctgc gacgaagcgt
     2641 gggcgatgta tcaccccgcc gggtctccat ccgtgcatct gctcaccttc ccggagcacc
     2701 tcgaggcttt tcgcgacgac gccctggcaa agaagtggga gaccgtccgc aatgtccgca
     2761 gcgtcgtgac cggcgcgctt gagctcgcgc gcgccgcgaa gcggatcggc tcttcgctgg
     2821 aggcatctcc gatcatttac gtagcggaca ggaagatgct ggcgacgctg ttcgacgtcg
     2881 atctcgccga ggtctgcatc acctcaaact acgaggtgca ggaggacgag gcgccggccg
     2941 gcgctttccg gctcgacccg gtgcccggcg tcgccgtcgt ggtggaagag gcgcagggcc
     3001 gaaaatgcgc ccgaagctgg aaaatccttc cgaccgtcgg cgacgacccc gaatatcccg
     3061 atgtctcgcc gcgcgacgcg caggccttgc gcgagtggaa ggcactgggg gtgagcgcgt
     3121 aatattcgct gtggtcccgg ccaggcgagg cgcgggccgg gacctgtaac cgccgtgttt
     3181 tgaaggtcgt ctaccagatg ggtctacgaa tggattcccc ggtatgggtc tccgctttcg
     3241 cggggtcgac agagcatttt ttgacggcgg catcgccatg aaatccccgc tccttccagg
     3301 cgtgcttgcc gcggtcgcga cgctggtcgt cgaccaggcg tcaaagctct ggctgctcca
     3361 tgtgttcgac atcggccacc gcggcacggt gaaggtgacg ccattcttcg accttgtcct
     3421 ggcctggaat atcggcatca gcttcggctg gtttcagaac gacaacccgg tcgcccagat
     3481 cgccctgatg gcaatcaagg ctctggcggt ggtcgcgctc gccatctgga tggcgaggtc
     3541 gcgcacggtg ctcgcaaccg ttgcgctggg gcttatcatc ggcggggcga tcggcaatgc
     3601 gatcgaccgc atcgcttatg gcgcggtgat cgattttgcg ctgtttcacg ttcagattgg
     3661 gcaaaatacc ttcaattggt acgtgtttaa cctcgcggat gtggccattg ttgccggagt
     3721 catggcccta ttgtatgatt cgtttatagg ggtacctgcc gcaaaagcgc cctgatcccg
     3781 gccgatcaaa acctagggtt ttgaataggt agagccatgc gcaagaccga agccaaagac
     3841 tggatgggac aacattcgtc gccgacgctg acgcgcgccg cacggctgtc agccgtcgtc
     3901 cttggcgtcg gcctgctcgt cgcctccggc gccgcgcgcg cccaggaggc cgatgatgag
     3961 gacaagacct tcgaggaaaa gattatcgaa ggcatcatga agggtatcgg cggcaccaat
     4021 atggacaaca cgggcatcga ctatcgcgag cgttcgccgc tcgtggtgcc gcccaagctt
     4081 gacctgccgc agccggccgc gagctcgacc gacgtgaagg cgccgaactg gccgaaggac
     4141 cccgacgagg cccgccgtaa ggccgcaatc gccgcgcgca agaaggcgaa caaggacccg
     4201 atggaagcaa gccgcatcct gagcccgagc gagctcaatg ccggcaggac cgccgcagga
     4261 accagcggcg gcaccaaggg ctctgattcg gcagtgccgg gcggcgatcc tggccggaat
     4321 gcgctcctga ccccctcgga actcggcttc gaaggccggt tctcgaacct gttcggcggt
     4381 aacaagacag agataaagga attcaaggct gagccgccgc gggactcgct gacccagcct
     4441 cctccgggat accagacgcc atcgtcgaat ttcgcatacg gcaccgggcc gaaagagtcg
     4501 ctcaacaagg aatacaaccc ggccgccggg aagtatggag aataagtcac gattcctctc
     4561 ggcttcgtga cgtcaggcgc accccgcagg ctgtagtgtg cctcgcttaa accgaac
//
LOCUS       DATLJT010000733         5203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1883763,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000733 DATLJT010000000
VERSION     DATLJT010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883763"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VM910_16805"
     CDS             complement(<1..198)
                     /locus_tag="VM910_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64223.1"
                     /translation="MSVSRLAAGVVALAIGAAVVTALAANPEVKPDLAAANSEANANP
                     ASLAAAKSDRLAIRPLKPAAAP"
     gene            500..691
                     /locus_tag="VM910_16810"
     CDS             500..691
                     /locus_tag="VM910_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64224.1"
                     /translation="MNGTTHTFDSFECAIHALAPTCENCGIRIVGHGLEKDGTFFCCE
                     HCAEAIGVTGLRDRVGAAA"
     gene            complement(725..952)
                     /locus_tag="VM910_16815"
     CDS             complement(725..952)
                     /locus_tag="VM910_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64225.1"
                     /translation="MPDMKAQTLTLHEGDKTALEYLGAAVVLQWSNLPEDLRKSLLQQ
                     ADSVGGLPFVANLHGQIEALIERVRGRGPGV"
     gene            complement(1007..2287)
                     /locus_tag="VM910_16820"
     CDS             complement(1007..2287)
                     /locus_tag="VM910_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64226.1"
                     /translation="MPAPELVIDYTQRTQFVAFHHRTERFACIVTHRRAGKTVACIHD
                     LQRAATECQRIRPRFAYLSPFLKQSKAVAWDYLRAAMAPLRAIGTSAHETELRVDYPN
                     GGQVRLYGADNPDALRGIYLDGIVLDEYADMDPRVWSEIIRPALADRQGWAVFIGTPK
                     GRNAFFELWQRSQSEQNWFSFMLKASETGLIPESELALAKRDLSEDQYAQEFECSFDA
                     AIVGSYYGKLMARAEQDQRIAPVPYDPAALVWTSWDLGIRDATAIWFAQMIGREIRII
                     DYYEASGVDLGHYVREIGARPYVYAGHIVPHDAQAKELGTGKSRLEVLESLGLKNISI
                     APLHRVEDGINAVRVFLPKCWFDANKCARGIDALKLYRAAYDDKLQALRPQPVHDWTS
                     HAADSFRYLALTLDRKATQIGFYRRIEYSQDGVA"
     gene            2603..3529
                     /locus_tag="VM910_16825"
     CDS             2603..3529
                     /locus_tag="VM910_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI64227.1"
                     /translation="MALRLLGYIELPPHRSNGGFDHADIHPPTDRIYVAHTSNDSLDV
                     IDCGRDRYIESIPGLAGVAGALVSEKRGLVFTSNRGENTISVFVPYDERNAFKIAVGV
                     KPNGLAFDPTRGILIAANVGDLSIPDSCSISVVDLGRRERIAEVKVPGRTRWAIYDAA
                     LEMFFVNIASPARIVAIDARDPTKISTEYEIPAAGPHGLDLDPAKGRLLCACDAGALF
                     AIDAASGRVLGEVPLSGSPDVIFLHPQSGHLYVAIGDPGVIDVIDIGTMRCEEVVPTE
                     AGAHTLALDRNRSKIYSFLPQSHRAAVFVDTT"
     gene            4152..>5203
                     /locus_tag="VM910_16830"
     CDS             4152..>5203
                     /locus_tag="VM910_16830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64228.1"
                     /translation="MDNAAPGPASLAALREAVRNGREPPPARIVARLTSYRWFVVGTV
                     CVGAFMGQVDSSIAQMLLPRLELEFDARLSTVSWVAVAYLLAMAAFLPIFGRLADMVG
                     RKLLYTGGFLLFVLSSALCGLAPSLPVLIAFRVAQGIGAALLSSNSVAIVVTAAGPAQ
                     RGRALGILSAAQAVGLSAGPALGGLVLDLLDWRWVFWINVPVGLAGTVLGWFVLPPTQ
                     DLPADSRFDWKGAFLIAPALTAVVAVLNEGYAWGATSPALLGCALLALILLTLFVRAE
                     QRADAPLIDLTLFRQSAFSAGNVAGLMGYAALFGLFFLMPFIFVRAYRDSTFAAGLRL
                     SIVPVMLGVVAPIAGAL"
BASE COUNT          913 a         1626 c         1665 g          999 t
ORIGIN      
        1 cggagcggcg gcgggcttga gcggacgaat cgcgagccgg tcgctcttgg cagcagccag
       61 gctggcggga ttggcgttcg cctcggaatt ggcagcggcg agatccggct tcacctccgg
      121 attggcggcg agcgccgtca ccactgcggc gccgatcgca agcgccacca cgccagctgc
      181 gagcctggag acggacattt gaccacccac ccagccgccc ccgttgcgcc ggagacgcgg
      241 tcgggggttc tgggagttag tcgcgtcggc gcggcgaaag gttcatgccc gcgggcgcgt
      301 tttcccgcca ttttcccgcc aaaatgccgc actcggcgcg aatggcggca aaagcgctgc
      361 catcgggacg taaaaacggc cggaaccggc ccggcgacgc cgggttatga cctcaaaccc
      421 cccaaaacca cggccagttg cgaggttgtc atggccagat gtgaaacctg tggaaacgac
      481 tacgacaagg caggtcagca tgaacggtac cacccacacc ttcgacagct tcgaatgcgc
      541 gatccatgcg ctggcgccga cctgcgagaa ctgcggcatc cgcatcgtcg gccacgggct
      601 cgagaaggac ggcaccttct tttgctgcga gcactgcgcc gaggccattg gcgtaaccgg
      661 cctgcgcgat cgcgtcggcg cggcagcgtg agcgggatcg ggacccaggc gccacgagca
      721 cgcgtcacac gcccggaccg cgcccgcgaa cacgctcaat gagcgcttca atctgcccgt
      781 gcagattggc aacgaagggt aatccgccga cggaatccgc ctgctgcaag agagactttc
      841 gaagatcctc gggcagattc gaccattgca ggacgacggc ggcgccaaga tactcgagcg
      901 cggttttgtc gccctcatgc aaagtgaggg tctgagcctt catgtcaggc atatatgctg
      961 ctcctacttt gcaccgatta tatagggtcg cgacgcgcgt gcgagatcac gccactccgt
     1021 cttgcgaata ttcgatgcga cgataaaagc cgatttgcgt cgccttgcgg tcgagcgtga
     1081 gcgcgaggta gcggaacgag tcggccgcgt gcgaggtcca gtcgtgtacc ggctgcggcc
     1141 gcagcgcctg cagcttgtcg tcgtacgcgg cgcggtagag cttgagcgca tcgatgccgc
     1201 gcgcgcattt gttcgcgtcg aaccagcatt tcggcaggaa gacgcgcacc gcgttgatgc
     1261 cgtcctccac ccggtgcagc ggggcgatgc tgatgttctt gaggcccaag ctttccagca
     1321 cctcgaggcg gctcttgccg gtgccgagct ccttcgcctg ggcatcgtgc ggcacgatgt
     1381 gcccggcata aacgtaaggc cgcgcgccga tctccctgac gtagtggccg agatcgacgc
     1441 cggaggcttc gtagtagtcg ataatcctga tctcgcgccc gatcatctgc gcgaaccaga
     1501 tcgctgtcgc gtcgcggatg ccgaggtccc acgacgtcca caccagcgcg gccggatcat
     1561 agggcacggg agcgatgcgc tgatcctgct cggcgcgcgc catcagcttc ccgtagtagg
     1621 atccgacgat cgcggcgtcg aacgagcatt cgaactcctg cgcgtattgg tcttcgctga
     1681 gatcgcgctt ggcgagcgcg agctcgcttt cgggaatgag gccggtctcg ctcgccttga
     1741 gcatgaagga aaaccaattc tgttccgact gcgagcgctg ccacagctcg aagaaggcgt
     1801 tgcgcccctt gggcgtgccg atgaagaccg cccagccctg ccgatccgcc aacgccggcc
     1861 ggatgatctc cgaccacacg cgcgggtcca tgtcggcgta ttcgtcgagc acgatgccgt
     1921 cgaggtagat gccgcgcaac gcgtcgggat tgtcggcgcc atagagccgc acctgcccgc
     1981 cgttggggta atcgacgcgc aactcggttt cgtgcgcgct cgttccgatc gcgcgcagcg
     2041 gcgccatggc cgcgcgcaga taatcccacg ccaccgcctt cgattgcttg aggaagggcg
     2101 agagatatgc gaagcgcggg cgaatgcgct ggcactcggt cgccgcgcgc tgcaagtcgt
     2161 ggatgcaggc caccgtcttg ccggcgcggc ggtgcgtcac gatgcaggcg aagcgctcgg
     2221 tcctgtggtg gaaggcgacg aactgagtgc gctgtgtgta gtcgatgacg agctcgggtg
     2281 ctggcatggg agcgtgcttg gccaacctgc atagggtggg ttaggcgcga aagcgcgtgg
     2341 tggaggaagc agaatggcgc ttgaggtcga gaaaaaattt tgggcgtgcc agagaagcaa
     2401 tacattgtcg ggagccgcga ttatctctta gttggtttgc gggcatccga gctgcgaggt
     2461 gtttggtggt ccgatcatgt cgattgcggc acccagggcg tgacctatgt cgacacttta
     2521 caccggaagg gtgttgctct ttgtccgacc agccacgccg cagtggccaa ttgctcattg
     2581 aaccgcaaag aggttagcag cgatggcact tcgtttactg ggctacatcg aactcccgcc
     2641 gcatcgatcc aatggcgggt tcgatcacgc tgatattcat ccacccacgg atcggatcta
     2701 cgtcgcacat accagtaatg attctctcga cgtgatcgac tgcgggcgag atcggtacat
     2761 tgaatcgatt cctggcctcg cgggagtggc aggtgcgctc gtatctgaaa agcgcggtct
     2821 cgtgttcaca agcaatcgcg gcgagaatac catttcggta ttcgtgccct atgacgagcg
     2881 gaacgctttc aagatcgccg taggcgttaa gccgaacggg ctcgcgttcg accccactcg
     2941 tggcatcctg atcgccgcga acgttggcga tctctcgatt ccggattcat gttcaatatc
     3001 ggtcgtggac cttgggcgac gggagcggat cgccgaagtc aaagtccctg gccgcacccg
     3061 ttgggccatt tacgacgccg cgctcgagat gttcttcgtc aatatcgcat ctccagcccg
     3121 gatcgtcgct atcgatgctc gtgacccgac caaaatatcg accgagtatg agattccggc
     3181 ggcaggaccg cacggactag acctcgatcc agccaaaggt cggttattgt gcgcatgcga
     3241 tgccggcgca ttgttcgcga tcgacgcagc gtcgggtcgc gtgctcgggg aagttcccct
     3301 aagcggctcc ccagacgtga ttttcttgca tccgcaatcc gggcatcttt acgtggccat
     3361 cggcgatcct ggggtaatag atgtcatcga tattggcacg atgcgctgcg aggaggtggt
     3421 accaaccgaa gcaggcgcac atacgctggc actcgaccga aacaggagca aaatctattc
     3481 gttcctgccg caatcgcatc gtgccgcggt atttgtcgat acgacttaac gccggcgcgc
     3541 cgatgagatg atcggacaag cacgtacctt tgctacggtg tgggcgcgat tggcagcgga
     3601 cttacggaca caatctttta tttgtccgct ttcagggcaa agcggacgcg aggggacggt
     3661 cacgaccgac ccggacggcc gcactggaac ggcgatccac gcgtagcttt ggcgaatacg
     3721 cttttttgga agattctcgt tacgcgagtc aaccgaaaaa tatctcgggt cgaagacggc
     3781 ggtgatggtc ggcgagtttt ggaacggtgc cgccctcagc aaacagcccg aaaatccgcg
     3841 cgcgcgagct aagataagca tctgttcatc cagaaagatt ttgccgcgcg cggaaacccg
     3901 gacagacgcg accggattgt ctcccgggga gaaacttcct cagaagaaaa agatgcgacc
     3961 atccaccggg ccgacttgcg gcatgctttc atcaaagggg gatggctgtc ggtacgattc
     4021 gcgactctgg aacccggaaa ttgctttgat taccctcatg tccggccatg ccgtgtccca
     4081 cgtcggacag gccggcaggt ctcacttgaa cagcaccttt tgcgccgcgg cgcccgcaac
     4141 gagcaaccaa catggataac gccgcgcccg gccccgcctc gctcgccgct ttgcgcgagg
     4201 ccgtgcgcaa cggccgcgaa ccgccgccgg cgcggatcgt ggcgcgcctt acgtcctacc
     4261 ggtggttcgt ggtgggcacc gtgtgcgtcg gcgccttcat gggccaggtc gattccagca
     4321 tcgcacagat gctgctcccg cggctcgaac tcgaattcga cgcaaggctc agcaccgtga
     4381 gctgggtcgc ggttgcctat ctcttggcga tggcagcgtt cctgccgatc ttcggtcgcc
     4441 tcgccgacat ggtgggacgc aagctcctgt acacgggcgg cttcctgctg ttcgtgctga
     4501 gctcggcgct ctgcggcctt gctccgagcc tgccggtgct catcgccttc cgggtcgcgc
     4561 agggcatcgg cgcagcgcta ctgtcgtcga acagcgtcgc catcgtggtg acggcggccg
     4621 gacccgcgca gcgcggacgc gcgctcggca tcctgtccgc agctcaagcc gtcggattga
     4681 gcgccggccc ggcgctcggc ggcctcgttc ttgacctgct ggactggcga tgggtgttct
     4741 ggatcaacgt cccggtcggc cttgccggca ccgtcctggg ctggttcgtg cttccgccca
     4801 cccaggactt gcccgctgac agccgcttcg actggaaggg cgcattcctg atcgcgcccg
     4861 cgctcaccgc cgtcgtggcc gtgctcaacg aagggtacgc gtggggggcg acgtcgcccg
     4921 cccttctcgg ctgtgcgctg ctggcgttaa tcctgctgac gctgttcgtt cgcgccgagc
     4981 aacgcgccga tgcgccgctg atcgacctca cgctgttccg gcaaagcgcg ttctcggccg
     5041 gcaatgtggc cggcctcatg ggctacgcgg cgctgttcgg cctgttcttt ttgatgccct
     5101 tcatcttcgt gcgcgcctac cgggattcga cgttcgccgc gggtctgcgg ctttccatcg
     5161 tcccggtgat gctcggcgtg gtggcgccga tcgccggcgc gct
//
LOCUS       DATLJT010000734        20310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3721595,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000734 DATLJT010000000
VERSION     DATLJT010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20310
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3721595"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VM910_16835"
     CDS             <1..372
                     /locus_tag="VM910_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI64229.1"
                     /translation="MNMSWQSVPRMLHLIGPARTKQAVIMADERISAKEAYEWGLVEE
                     VAKAGKAFEAAMAMAEKVARQPPISVAMTKLTVNRLAHALDDLASHMDIDQFALASMT
                     EDHKEGVAAFIERRKPRFRGR"
     gene            complement(393..1427)
                     /locus_tag="VM910_16840"
     CDS             complement(393..1427)
                     /locus_tag="VM910_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64230.1"
                     /translation="MSRRQFIAGTLAIGAAAAWSGGPTPALALERTLIDTHHHFYPPG
                     YQKLWLDYEDQRKIPHFAGQVAWSKQKAIEDMDRAGIRTAMLSVASTPGVWFDLGPDK
                     AGELARTCNDFAAEMMRDYPGRFGLFATLSMLDIDQTLKEIEYALDTLRADGVGLQTN
                     YGDKWLGNAVYKPVFDELNRRKVVVYVHPLVASCCAQLSVGTFPAVIEVPHDTTRTVT
                     SLLLSGGFARWRDIAWLFSHAGGTVPMMAGRIDAFYGARPNIKEFAPDGILGELKRLN
                     YDTANATSAPAIAALMKLVPASRVTYGTDYPYFQLDQMKNLEQLGLDASDLKAIGSEN
                     AIRLIPRLKA"
     gene            1868..2887
                     /locus_tag="VM910_16845"
     CDS             1868..2887
                     /locus_tag="VM910_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI64231.1"
                     /translation="MSFSRRTLLKASAASAVVGGLGAPRIARAQAGEFTYKYANNLPD
                     SHPMNIRAKEMAAAIKAETNGRFDLQIFPNNQLGSDTDMLSQIRSGGIEFFTLSGLIL
                     STLVPATSINGIGFAFPDYDTVWKAMDGDLGAYVRGEIKKAGLEVMDKIWDNGFRQTT
                     SSTRPINGPEDFKGFKIRVPVSPLWTSMFKAFDASPASINFSEVYSALQTKIVEGQEN
                     PLAIISTAKLYEVQKYCSLTNHMWDGFWFLANRRAWEAIPQDMRAIVVKNVNAAAVNE
                     RADVAKLNASLQQELAAKGLVFNQPDTAPFREKLRSAGFYAEWKGKYGEQAWALLEKA
                     AGKLS"
     gene            2903..4789
                     /locus_tag="VM910_16850"
     CDS             2903..4789
                     /locus_tag="VM910_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVI64232.1"
                     /translation="MARIEVSEFAVGEVTLEPPSRPSLAGSIESWLGLLVEIPAAVLV
                     VAEIVILFTGVVARYVLRQPLIWSDELASILFLWLAMLGAAVAFRRSEHMRMTAVVAR
                     AGDRMQAYLGLFATCAALAFLLMIVGPAYEYAHEESFITTPALQISNSWRAAALPVGT
                     CLMAVFALLRLLRSGDPKLVISAFISVALLIALFWIAQPWLRPLGNINLVIFFVGVAG
                     FCVFSGVPIAFSFGLAIFGYLALTTRTPLVVLVGRMDEGMSHLILLSVPLFVFLGLLI
                     EMTGMARAMVAFLASLLGHIKGGLHYVLVGAMYLVSGISGSKAADMAAVAPVLFPEMK
                     SRGARPGDLVALLAATGAQTETIPPSLVLITIGSVTGVSISALFTGGLLPGVVLAITL
                     SALVWWRYRREDLSHVKRARLREIATTFVVALPALALPFVIRAAVVQGVATATEVSTI
                     GIVYGFVVGLLVYRRFDWRRLLPMLIETASLSGAILLIIGTATGMAWGLTQSGFSRAL
                     ASAMTGLPGGAASFIAVSIVAFTILGSVLEGIPAIVLFGPLLFPIARAVGVYEVHYAM
                     IIILAMGIGLFAPPFGVGYYAACAIGRVDPAQGIRPIWGYMLALLVGLVIVAIFPWIS
                     IGFL"
     gene            4810..6459
                     /locus_tag="VM910_16855"
     CDS             4810..6459
                     /locus_tag="VM910_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HVI64233.1"
                     /translation="MSGKPNQYEIGLDKTPANYVPLTPLSFIARSAAVYPNHTSAVYE
                     GRRFTWAETYARCRRFASYLSSKGIGEGDTVAAMLPNVPAMNELHFAVPMAGAVLNAL
                     NIRLDAPSIAFQLDHGGAEIILVDPEFSGVIAEALTLMKGPKPFVIDVDDASFSGGKH
                     IGGIEYETALTQGDPGFVPRPPEDEWDAIALSYTSGTTGNPKGVVTHHRGAYLNAVSN
                     ILAGNLGQHPVYLWTLPMFHCNGWCFPWTVAAGAGVNVCLRKVEPTKIFDLIKTHGVT
                     HMCGAPIVYNTLINAPDAPKGKKDKPVIGLIAGAAPPVAVLEGAENIGIKLTHVYGLT
                     EVYGPASVCAEQPGWDQLSPPDRAHFKRRQGVPYPLEEAVTVLDPQTMREVPRDGETI
                     GEVMIRGNIVMKGYLKNENATRQAFEGGWFHTGDLGVLDEHGYVIIKDRSKDIIISGG
                     ENISSVEVEDVLYKHPAVLFAAVVAKPDAKWGEVPCAFIELKEGAEATEAEIIAYCRS
                     HMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNQADSAKAISA"
     gene            complement(6536..7843)
                     /locus_tag="VM910_16860"
     CDS             complement(6536..7843)
                     /locus_tag="VM910_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64234.1"
                     /translation="MDAALQRSTMRKVYLRLLPFAVLSYVLAYIDRINVSFAGLTMRS
                     DLGMSAATFGFAVGMFYWGYFIFEVPSNVILEKVGARIWIARIMITWGILAGLTAAVS
                     GSTGFAIVRFLLGVAEAGFFPGIILYFTYWFPSRHHARIVSGFLIGLPVAVALGAPIS
                     TALLGLDGLFGLRGWQIMYIAEAVPTVFIGVLTYFVLTDRPQEAKFLSEEERNWLVST
                     IAAERRATEAVRKFTMWEALYNPKVLLLSLNYLGIVTASLGMLIFIPQIIKSLGNYSN
                     MTVGWLTMIPYICGAIAMVVWGRISDRMNERRWNLFFGCVLSTVGLVIAGMTMGTWGA
                     LVGMSIAAMGFYGSKGPFFAMPPMFLSGPALAAGIAWINSIGNLGGFFGPSYVGLMKD
                     LTGGYAGGLYGLALLGLVAAIVCALFLHIPKRIPSEAVGAPAE"
     gene            complement(7998..8795)
                     /locus_tag="VM910_16865"
     CDS             complement(7998..8795)
                     /locus_tag="VM910_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI64235.1"
                     /translation="MTLTLTILGCGSSAGVPRPALGWGKCDPNNPKNRRRRCSLLVER
                     TGAHGTTRIVIDTSPDLREQLIDAKVDHIDAVFLTHEHADQTHGIDDLRSVVLHMRRR
                     IPTYFNQSTAKDIMARFSYCFISPEGSDYPPILTRHGIEAGESQEILGKGGAVTLSAF
                     IMQHGNIPALGYRIGDAAYTPDVSDIPRESWGALEGLDLWIIDGLRYAPHPSHFSVDD
                     ALTWIERFKPRQAVITNMHSDLDYEELRQKLPKGVVPAYDSMRFTMK"
     gene            complement(8792..9580)
                     /locus_tag="VM910_16870"
     CDS             complement(8792..9580)
                     /locus_tag="VM910_16870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HVI64236.1"
                     /translation="MLVDSHCHLDFPDFSGDLDAIVARAAQAGIGRIVTISTRVRRLS
                     ELIAIAERFPNVYCSVGTHPHHADEEDGIAPEELIELTGHPKVVALGEAGLDYFYDNG
                     SPQAQARGFRAHIAAARATGLPLVVHTRDADEDCGRILQEETAKGPFRAVLHCYTGGR
                     ELAMTAIELGLSISFTGILTFKKSEALRALAAELPADRIMVETDAPYLAPGKFRGKRN
                     EPAYVVEVAKVLAEARGVSLEEISRQTTENFFRLFSKVPSPKTA"
     gene            complement(9641..11266)
                     /gene="metG"
                     /locus_tag="VM910_16875"
     CDS             complement(9641..11266)
                     /gene="metG"
                     /locus_tag="VM910_16875"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HVI64237.1"
                     /translation="MPNKPEQKSAATARQAEPATSRNAASRGNTFYITTAIAYPNGTP
                     HIGHAYEAIATDALARFQRLDGKDVFFLTGTDEHGLKMVQTAENEGLTTADLATRNSR
                     RFRAMDERLNVSFDRYIRTTEAQHHRSSQEIWRRMAANGDIYIDTYAGWYSVRDEAYY
                     AEEETVLGDDDVRRGPQGTPVEWVEEKTYFFRLSAYQDELLALYESQPDFIGPDARRN
                     EVVSFVKRGLKDLSISRTTFDWGVKVPDDPAHVMYVWVDALTNYITGVGFPDENDANW
                     RFWPADLHIIGKDIIRFHAVYWPAFLMSAGIALPKRVFAHGFLFNRGEKMSKSVGNVV
                     DPFNLADQYGVDQIRYFFLREVAFGQDGSYNHEAIVARINADLANDLGNLAQRSLSMI
                     AKQLGGVLPEPGAFSENDKAMLAQADGMIAIAREGMATQQIHTWLNAVWAVVAEANRY
                     FAGEAPWALAKTDPARQRTVLYVTAEVIRQIAILTQPVMPSAAEKLLDSLGIPKGEAR
                     TFATLGGTSRIAPGTTLPAPQAVFPRYIEPTAA"
     gene            11256..11540
                     /locus_tag="VM910_16880"
     CDS             11256..11540
                     /locus_tag="VM910_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64238.1"
                     /translation="MLGKACFGSDLALGAFRFVVDVFAGFLAAFFATVLADFFAALFA
                     VFLALFRPRFFAAAGLAAARFAAVLAFFFFGDFLATTKSSNEPERDCWGR"
     gene            complement(11553..12596)
                     /locus_tag="VM910_16885"
     CDS             complement(11553..12596)
                     /locus_tag="VM910_16885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HVI64239.1"
                     /translation="MSARRVEAEISAPHPRETFELFGHREAETALLNAYKSGRIPHAW
                     LIGGAQGIGKATLAYRMARFVLTHRNPLAAEVQRATTLFVDPNDASARHIAAGSHGGL
                     LTLERTLNERGVMRTVITVDETRETISFFGSTSAVEGWRVCIVDTLDELNPNAANALL
                     KILEEPPQQSLFFLVSHAPARVLATIQSRCRKLSLRPLAAADVIAAAAKATGVASDDP
                     ALIEAANASEGSVARAITLLGGDALKLQQRTAALLATLPQVDPRELHALGDALGTSDR
                     VALAAFVDSIDRWVAERLRAEGNANTNLPRLARLAEVWEKIARAARDTQDYNLERKPL
                     VFSVFSMLAEATR"
     gene            complement(12593..13285)
                     /gene="tmk"
                     /locus_tag="VM910_16890"
     CDS             complement(12593..13285)
                     /gene="tmk"
                     /locus_tag="VM910_16890"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVI64240.1"
                     /translation="MIEPAIKRPPGRGRFITFEGGEGSGKSTQIKKLAERLEAAKLHV
                     IVTREPGGSIGAEIIRHLVLSGMGKLLGPDAETLLFAAARDDHVRTVIQPALNQGTWV
                     LCDRFFDSTRVYQGKVGHVSPGIVNAMQRVTIGDLKPDLTVILDVPVEIGLKRAAARR
                     EGAAPDRFESEDIRFHQELRDAYHEIAAREPERCVLIDAMADADTVAASVWAALRGRF
                     FAEPTGTVASSA"
     gene            complement(13288..14442)
                     /locus_tag="VM910_16895"
     CDS             complement(13288..14442)
                     /locus_tag="VM910_16895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVI64241.1"
                     /translation="MLHAANQSVQGAKKEEGGFDGDAPTAILIEASSGSVLFEKNADE
                     LRAPSSMMKLVTAEVVFHAIQEGKVKLTDEYRISENAWRRGGAPAGGSTMFAVLNSKI
                     SVDDLLHGAIIQSGNDACIALAEGMAGNERIFASEFMTKRARELGLTRSTFGNANGLP
                     DPGNKMTVRELAKLARYIIQTYPEFYKLFGEKEFTWNKIRQQNRNPLLNSMEGADGLK
                     TGYTKEGGYGMVGSAVQNGTRLIVAINGLEDPDDRATEAKKMLEWGYRNFETRTLFAA
                     DQPLGYARVFGGESRSVKLSSPEPIKVMVSKQGNDKLIARLVYSGPVRAPIKAGQQIG
                     VVRVWRGGNVAMEAPVYAAEAIGTGSTVRRAIDGASELAIGMFRAGAEKL"
     gene            complement(14762..15700)
                     /locus_tag="VM910_16900"
     CDS             complement(14762..15700)
                     /locus_tag="VM910_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVI64242.1"
                     /translation="MGFLRSDPAIRAAHGAVAVCVCLVLGNCASSSKFASRIDPKYGV
                     SSSPRVVADGEPVPKGGGTYRVGKPYVVAGRVYVPEEDVNYREEGLASWYGNDFHGRL
                     TANGEVFDMDSLTAAHPTLPMPCYARVTNLSNGKSLIVRVNDRGPYHGNRLIDVSNKA
                     AELLEFKGNGVARVRVEYVARAPLEGSDDRQLIATLRTGVPAPSPSLVRVASARPFVP
                     ETGAYGRPIRGDIPLPQDRPYSLGNTSADYASINATSEMSASSRARSGGRVLDNRRAV
                     SYEDDARYTADYQSVSAYAPVDPRGPSEILSGRGLY"
     gene            16694..16822
                     /locus_tag="VM910_16905"
     CDS             16694..16822
                     /locus_tag="VM910_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64243.1"
                     /translation="MLAEKFFLVLEALIKTQQTNADGSVRVVSTSPHVPVKLPERK"
     gene            17046..17366
                     /locus_tag="VM910_16910"
     CDS             17046..17366
                     /locus_tag="VM910_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVI64244.1"
                     /translation="MRESFARLVAAALVIAAAAVPAHARTVRILMENLVLRPADTRAK
                     VGDTVEWINDDMVAHTATARSGDFDVIVPSKRTASFVLLKVGTVEYYSRFHPIMKGRL
                     RIKP"
     gene            complement(18693..19004)
                     /locus_tag="VM910_16915"
     CDS             complement(18693..19004)
                     /locus_tag="VM910_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64245.1"
                     /translation="MPRVVVQYVSKPRPGSNLATILEIAKEAAAIWRKHGGNVTYWSI
                     MGGEVGNLAFTVGFESFEDYGRVLAAVFADPATAEWQAKRLKAGQSDWVRANTAFELE
                     L"
     gene            19722..19946
                     /locus_tag="VM910_16920"
     CDS             19722..19946
                     /locus_tag="VM910_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64246.1"
                     /translation="MNKILSPEQTARLERQRLAAEDGAKALQEVAERSVAVRENMARL
                     RALRLAKEAKAVRTRTATENQRPNSRRDRR"
     gene            20174..20263
                     /locus_tag="VM910_16925"
     tRNA            20174..20263
                     /locus_tag="VM910_16925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20208..20210,aa:Ser,seq:tga)
BASE COUNT         3818 a         6219 c         6220 g         4053 t
ORIGIN      
        1 atgaacatga gctggcagag cgtgccgcgc atgctgcatc tgatcgggcc ggcgcgcacc
       61 aagcaggccg tgatcatggc ggacgagcgg atttccgcga aggaagccta tgaatgggga
      121 ctggtggagg aggtggccaa agccggcaaa gcctttgagg ccgccatggc gatggccgag
      181 aaagtcgcgc gccagccgcc catctcggtg gccatgacca agcttacggt taatcggctg
      241 gcgcacgcgc tcgacgatct cgcaagccac atggatatcg accagtttgc gctggcgagc
      301 atgaccgagg atcacaagga aggcgtcgcg gcctttatcg agcggcgcaa gccgcgcttc
      361 aggggacgct aggggacgca caaaacgaca gcctacgcct tcaagcgcgg gatcaaccgg
      421 atcgcgtttt cgctgccaat cgccttgaga tcggaggcat caagcccgag ttgctccagg
      481 ttcttcatct ggtcgagttg gaagtagggg taatccgtac cgtaggtcac cctcgaggca
      541 ggcacgagct tcatgagcgc tgcgatcgca ggggcgctgg tcgcgttcgc agtatcgtag
      601 ttgagacgct tcagctctcc caaaatgcca tccggagcga actccttgat gttgggccgc
      661 gcgccgtaaa aggcgtcgat ccggccggcc atcataggga cggtgccgcc tgcatgcgag
      721 aacagccaag cgatatcccg ccaccgcgca aacccgcccg acaggagcaa gcttgtgacc
      781 gtgcgtgtcg tatcatgggg gacttcgatc acggccggga acgtgccaac gctgagctgt
      841 gcgcaacagc ttgcgaccaa gggatgcaca tagaccacca ccttgcggcg attcagttcg
      901 tcgaacaccg gcttatacac cgcgttccca agccatttgt cgccatagtt cgtctgcagg
      961 cccacgccgt cagccctaag cgtatcgagg gcatattcga tctccttgag cgtctgatcg
     1021 atatcgagca tcgacagggt tgcaaacaac ccgaaccggc ccggatagtc ccgcatcatc
     1081 tcggcggcga aatcattgca cgtccgcgca agctcgcccg ccttgtccgg cccaagatcg
     1141 aaccaaacgc cgggagttga tgccaccgac agcatggcgg tgcgaatgcc tgccctgtcc
     1201 atgtcttcga tcgctttctg cttcgaccag gcgacctgac ctgcaaagtg tggaatcttg
     1261 cgctggtcct cataatccag ccaaagcttt tggtatcccg gcggataaaa gtggtgatgg
     1321 gtgtcgatca gcgtccgctc gagggcgagg gcgggcgtag gccctcccga ccaggccgcg
     1381 gcggcgccga ttgcgagtgt gccggcgatg aactgacggc gcgacaatcc cgatgtccca
     1441 cagcatgcgc agccctgaag gtggttccgc gcggaagcca aattcgtcat ccgtggtctc
     1501 cttaacgcag gctgcagcac gctcgtgttg atgccacggc gtttcggcgg ggagtagtag
     1561 gaatcctgtg aacggaatgc gaagccagcg gaccttcatc gccagcgcgg cccacggccg
     1621 gatatccacg attcgcataa ggtatattat ggaatatatt tatccatagc aggatcagac
     1681 agttagctca aattcttgcg aaaaagcgcc aattggtggg cctcgtcggg cctttggcgc
     1741 gagcgccctg aacgaggctc atgggcggcg gcaaaagccg atattgccgc cgcgccctga
     1801 cgctgcaata agcgcgctgg tcggacttcc gaacctcaac gacaaaaagt ctcatgggag
     1861 ggaaaaaatg agtttttcac gacgcacgct gctgaaagcg tccgcggcat cggctgtggt
     1921 tggtggtctc ggcgcgccca ggattgcgcg cgcgcaagcc ggcgagttca cttacaagta
     1981 cgccaacaat ctcccggact cccatccgat gaacatccgc gccaaggaaa tggcggcggc
     2041 gatcaaggcc gaaaccaacg gacgcttcga cctgcagatc tttccgaata accagcttgg
     2101 gtccgatacg gacatgctga gccagattcg atcgggaggc atcgagttct tcacgctatc
     2161 gggtttgatc ctttcgacgc tggtgcccgc gacttccatc aacggcatcg ggttcgcttt
     2221 ccccgactac gacacggtct ggaaggccat ggacggcgac ctcggcgcct atgtgcgcgg
     2281 cgagatcaag aaggccggtc tcgaggtgat ggacaagatc tgggacaacg gattccggca
     2341 gacgacgtcc tcgaccaggc cgatcaacgg tccggaagat tttaagggtt tcaagattcg
     2401 cgtaccggtg tcgccgctct ggacctcgat gttcaaggct ttcgatgcgt cgcccgcctc
     2461 gatcaatttc agcgaagtct attcggcgct acaaaccaag atcgtcgagg gtcaggaaaa
     2521 tccgctcgcg attatctcga ccgcgaaact atacgaagtg cagaaatact gctcgctgac
     2581 caatcatatg tgggacggtt tctggttcct ggccaatcgg cgcgcctggg aggccattcc
     2641 gcaggatatg cgcgcgatcg tcgtcaagaa cgtcaacgcg gcagccgtga acgaacgcgc
     2701 cgacgtcgcc aaactgaacg caagcctgca gcaggagctt gcagcgaagg gccttgtatt
     2761 caaccagccg gatacggcgc cgttccgcga gaagctgcgt tccgccggtt tctacgccga
     2821 atggaaaggt aaatacggcg agcaggcatg ggcgctgcta gaaaaggccg ctggcaagct
     2881 atcgtaagtt tcgagggccg taatggctcg catcgaagtg tcggaatttg cggtcggcga
     2941 ggtgaccctc gagccaccaa gccgcccttc ccttgccggg tcgatcgaat cctggctcgg
     3001 gcttctggtc gaaatcccgg cggcggtcct cgtcgtcgcc gaaatcgtga tcctgttcac
     3061 cggcgtcgtc gcacgctacg tcctgcgcca gcctctgatc tggtcggacg aactggcatc
     3121 gatccttttc ctgtggctgg cgatgttggg cgcagcggtg gcgttccgcc gctccgagca
     3181 tatgcggatg accgcggtgg ttgcgcgcgc cggcgatcgg atgcaggcct atctcggcct
     3241 ctttgcgacc tgtgcggcgc ttgcgtttct cctgatgatc gtcggcccgg cctacgaata
     3301 tgcccatgag gaaagcttca tcaccacgcc ggcactgcag atttccaata gctggcgtgc
     3361 cgcagcgctg ccggtcggca cctgcctgat ggctgttttt gcgttgttgc ggcttctgcg
     3421 cagcggcgac ccgaagcttg tgatatcggc cttcatctcg gttgcgttgc tgatcgcgct
     3481 gttctggatc gcgcagccgt ggctgcgtcc gcttggcaat atcaacctcg tgatcttctt
     3541 tgtcggtgtc gccggctttt gcgtgttttc gggcgtgccg atcgcgttca gctttggact
     3601 cgcgattttt ggctatctcg cgctgacgac acgcacaccg ctggtggtgc tggtcgggcg
     3661 gatggacgag ggcatgagcc atctgatcct gctctcggtg cctctgttcg tgttcctggg
     3721 gcttttgatc gagatgaccg gcatggcgcg agccatggtg gcgtttctgg caagcctgct
     3781 cggccatatc aaagggggcc tgcattacgt gctcgtcggc gcgatgtatc ttgtctccgg
     3841 catttcaggt tcgaaggcgg ccgacatggc cgccgtcgca ccggttctgt ttcccgagat
     3901 gaaatcacgc ggcgcccgcc cgggcgacct tgtcgcgctg cttgccgcga ccggcgcgca
     3961 gaccgaaacc atcccgccaa gtctcgtgct gatcaccatc ggctcggtca ccggcgtctc
     4021 gatctcggcg ctgtttaccg gcggcctctt gcccggcgtg gtgcttgcga tcacgctgtc
     4081 ggcgctggtc tggtggcgct atcgccgcga ggacctgagc catgtgaagc gcgcaaggct
     4141 ccgcgagatc gccacgacgt ttgtcgtcgc actgcccgcg ctggcattgc ctttcgtgat
     4201 ccgcgccgcc gtggtccagg gcgttgcgac cgcaaccgaa gtctccacca tcggcatcgt
     4261 ctatggcttc gtggtcggcc tgttggtcta ccgccgtttc gactggcgca ggcttttgcc
     4321 gatgctgatc gagaccgcgt cgctgtcggg ggcgatcctg ctgatcatcg gaaccgccac
     4381 cggaatggca tggggcctga cgcaatcggg cttttctcgt gcgctcgcct ccgccatgac
     4441 cggcctgcct ggcggcgcgg cctccttcat cgcggtctcg atcgtggcct tcactatcct
     4501 cggcagcgtg ctggaaggga ttcccgcaat cgtgctgttc ggaccgctgc tgtttccgat
     4561 cgcccgggcc gtcggcgtgt acgaggtgca ctatgccatg attatcatcc tggcgatggg
     4621 gatcggattg ttcgccccgc ccttcggcgt cggttattat gcggcctgcg cgatcgggcg
     4681 ggttgacccc gcacaaggta ttaggccgat ctggggctac atgctggcgc tcctggttgg
     4741 acttgttatc gttgcgatct ttccgtggat ctcgatcggg ttcctgtgat ttgaagtggg
     4801 agatgaccga tgagcggcaa accgaaccaa tatgaaatcg gtctggacaa gacgccggcc
     4861 aactacgtgc cgctgacgcc gctcagtttc atcgcccgta gcgcggcggt ctatccgaac
     4921 cacacgagtg cggtctacga aggccggcgc tttacctggg ccgaaactta cgcacgctgc
     4981 cgtcgctttg cctcttatct ttccagcaaa ggcattggcg agggcgacac ggtcgcggcg
     5041 atgctgccga acgtgccggc gatgaatgaa ctgcatttcg ctgtccccat ggcgggcgca
     5101 gtgctgaacg cgctcaacat ccgccttgac gcgccctcga tcgccttcca actcgaccat
     5161 ggcggtgccg agatcatcct ggtcgatccg gagttttcgg gcgtgatcgc ggaagcgctg
     5221 accctgatga aagggccaaa gccgttcgtg atcgacgtcg acgatgcatc gttttcaggc
     5281 ggaaagcaca tcggcgggat cgaatatgag acggccttga cgcagggcga tcccggcttt
     5341 gtccctcgcc cgcccgaaga cgaatgggac gcgatcgcgc tctcctacac atcgggcacc
     5401 acgggcaatc ccaagggcgt cgtcacccat catcgcggtg cgtatctgaa cgcggtcagc
     5461 aacatccttg caggcaatct cggccagcac ccggtctatc tctggacctt gccgatgttc
     5521 cactgcaacg gctggtgttt tccgtggacg gtcgcggccg gtgccggtgt caatgtctgc
     5581 ctgcgcaagg tcgagccgac caaaatattc gatctgatca agacgcacgg cgtcacccac
     5641 atgtgcggtg cgccgatcgt ctacaatacg ctgatcaacg cccccgatgc tccgaagggc
     5701 aagaaggaca agccggtgat tggcctgatc gccggtgccg cgccgcccgt ggccgttttg
     5761 gaaggcgccg aaaacatcgg catcaagctc acccatgtct atgggctgac cgaagtctac
     5821 ggtcccgcct ccgtttgcgc tgaacagccg ggctgggatc agctttcgcc gccggatcgc
     5881 gcgcatttca aacggcggca gggtgtgccc tacccgctcg aggaagccgt caccgtgctc
     5941 gacccgcaaa ccatgcgcga ggtgccgcgc gacggtgaga ccatcggcga ggtgatgatc
     6001 cgcggcaata ttgtgatgaa gggctatctg aagaacgaga acgcgaccag gcaagccttt
     6061 gaaggcggct ggttccacac aggcgatctc ggcgtgctcg acgagcacgg ctatgtcatc
     6121 atcaaggatc gctccaagga catcatcatc tccggcggcg agaacatctc atccgtcgag
     6181 gtcgaggacg tgctttacaa gcacccggct gtgctgtttg ccgccgtcgt cgccaagccg
     6241 gatgcgaaat ggggcgaggt gccctgtgcc ttcatcgaat tgaaggaagg cgcggaagcg
     6301 acggaagcgg agatcatcgc ctattgccgc agccacatgt ccggcttcaa gacgccgaag
     6361 gtcgtggtgt tcgggccgat ccccaagaca tcgaccggaa aaatccagaa attcctgctg
     6421 cgcaatcagg ccgactccgc gaaggcgatc tcggcttagc agtgattgcg agcgaggcga
     6481 caactccccg caatgacgag ccacgcgcga cccggcacgc gtggccggtg cgcgatcatt
     6541 cggccggtgc gccgacggct tccgacggga tgcgcttggg aatgtgcagg aatagcgcgc
     6601 atacgatcgc tgcgaccaga ccaagcagcg ccaatccata aagtccgccc gcgtaaccgc
     6661 cggtcaaatc tttcatcagg cccacataag acggcccgaa gaagcctccg agattgccga
     6721 tggaattgat ccaggcaatg ccggcggcga gcgcgggtcc ggagagaaac atcggcggca
     6781 tcgcgaagaa tggacccttg gagccgtaaa agcccatcgc ggcgatcgac atgccgacga
     6841 gcgcgcccca agtccccatg gtcatgccgg caatgacaag cccgaccgtc gaaagcacgc
     6901 agccaaagaa cagattccag cgccgctcat tcatgcggtc cgagatgcgg ccccacacca
     6961 ccatggcgat ggcaccgcaa atatacggga tcatggtgag ccagccgacc gtcatgttgc
     7021 tgtaattacc cagcgacttg atgatctgcg gaatgaagat cagcatgccg aggcttgcgg
     7081 taacgatgcc gagatagttc agagagagca gcagcacctt tggattgtag agcgcttccc
     7141 acatggtgaa tttgcgcact gcttccgtgg cgcgacgttc ggcggcgatc gtgctgacga
     7201 gccagttacg ttcctcctcg ctgagaaatt tggcctcctg cggtcgatcg gtgagcacga
     7261 aatatgtcaa gacgccgatg aaaacggtcg gaacggcttc ggcgatgtac atgatttgcc
     7321 agcctcgcag gccaaacaag ccatcgagac caagcaatgc cgtcgatatc ggggcgccga
     7381 gcgccaccgc aaccggcaga ccgatcagga agccggacac gatccgcgcg tgatggcggc
     7441 tcggaaacca gtaggtgaag tagagaataa tgccggggaa gaacccggcc tcggcaacgc
     7501 cgagcaggaa tcgcacgatc gcaaagcccg tcgaaccgct gaccgcggca gtcaggcctg
     7561 ccaggatccc ccaggtgatc atgatccggg cgatccatat cctggcgccc accttctcca
     7621 ggatcacgtt gctcgggacc tcgaagatga agtagcccca gtaaaacatg ccgaccgcga
     7681 agccgaaggt ggctgccgac atgccgagat cgctgcgcat ggtgaggcct gcgaagctca
     7741 cattgatgcg atcgatatag gcgagcacgt aggacaggac cgcaaacggc aataggcgca
     7801 gatagacctt gcgcatcgtt gatcgttgca gcgcggcgtc catcggtttc ctccctgaag
     7861 gaatatgtgg acgctgcaac ttacccttaa gttcccaccc tgtcacggca accggcttga
     7921 gcaaaatggc gcaggctgca agctatgttg cagcgcgttc gtacactcgc ggcgtgccaa
     7981 gctcgggacg cggtccccta cttcatcgtg aagcgcatgc tgtcataggc cggcaccacg
     8041 ccttttggca gcttttgccg caactcctcg tagtcgaggt ccgaatgcat gttggtgatg
     8101 acggcctgcc gcggcttgaa gcgctcgatc caggtgagcg catcgtccac actgaaatgg
     8161 cttggatgcg gcgcataacg caaaccatcg atgatccaga gatcgaggcc ttccagcgcg
     8221 ccccagctct cgcgcggaat gtcgctgaca tcgggcgtat aggccgcatc gccgatccgg
     8281 tagccgagtg ccgggatatt gccgtgctgc atgatgaagg cggaaagcgt caccgcaccg
     8341 cccttcccca gaatttcctg gctctcgccg gcctcaatcc catgtcgggt caggatcggt
     8401 ggataatcgc tgccctcggg cgagatgaag caataggaga accgcgccat gatgtccttc
     8461 gcggtcgatt gattgaaata ggtcgggatg cgccggcgca tgtgcagcac caccgagcgg
     8521 agatcgtcga tgccgtgagt ctggtcggca tgctcgtgcg tcaaaaacac tgcgtctatg
     8581 tgatcgactt ttgcgtcgat cagttgctcg cgcaggtcgg gcgaggtgtc gatcacgatt
     8641 cgcgtggtcc cgtgcgcgcc agtccgttcc acaagtagcg agcagcggcg ccgccggttt
     8701 tttggattgt tcggatcgca tttgccccag ccgagcgcgg gacgcggaac gccggccgaa
     8761 gagccgcagc ccaggattgt cagggtcagt gtcatgcggt tttcggcgac ggcactttgg
     8821 agaacaggcg aaagaagttt tccgtggtct gccgcgagat ttcttccaat gaaacgccgc
     8881 gcgcttccgc cagcaccttc gcgacctcga cgacataggc aggttcgttg cgcttgccgc
     8941 ggaatttgcc gggcgccaga tagggcgcat cggtctccac catgatgcgg tcggccggca
     9001 attcggcagc aagcgcgcgc agggcttccg acttcttgaa ggtcaaaatg cccgtgaatg
     9061 aaatcgaaag cccaagctcg atcgccgtca tcgcaagctc gcgtccgccc gtgtagcaat
     9121 gcagcaccgc gcgaaacggt ccttttgccg tctcctcttg caggatgcga ccgcaatcct
     9181 cgtcggcatc gcgggtgtgg acgacgagcg gcagccccgt cgcccgcgcg gccgcgatat
     9241 gcgcgcgaaa gccgcgcgcc tgcgcttgcg gcgagccgtt gtcgtagaaa tagtcgagcc
     9301 ccgcctcccc cagcgccacc accttcggat gcccggttag ttcgatcaat tcttccggcg
     9361 caattccgtc ctcttcgtcg gcgtgatgcg gatgagtgcc aaccgagcaa tagacgttcg
     9421 gaaagcgctc ggcgatcgcg atgagttcgg aaagccgccg cacccgcgtc gagatcgtca
     9481 cgatgcggcc aatgcctgct tgagctgccc gcgcgacaat ggcgtcgaga tcgccggaaa
     9541 agtccggaaa atccagatgg cagtgactgt cgacgagcat ggcgctagtg ccccgttttc
     9601 gtagttctca ggctctcgcg gaaaatcggc ggcactgcca ctatgccgcc gtcggctcaa
     9661 tgtaacgcgg gaacaccgct tgcggcgccg gcagcgttgt gccgggcgca atccggctcg
     9721 ttccgccgag cgtggcgaag gtccgcgcct cgcctttggg gatgccgagg ctgtcgagca
     9781 gtttctccgc ggccgaaggc atgaccggtt gggtcaggat cgcgatctgc ctgatcacct
     9841 cggccgtgac atagagcaca gtgcgctggc gcgccggatc cgtcttcgcg agtgcccacg
     9901 gcgcttcgcc ggcgaaatag cggttggctt ccgccaccac ggcccagacg gcgttgagcc
     9961 aggtgtgaat ctgctgcgtc gccatcccct cccgcgcgat cgcgatcatg ccgtcggcct
    10021 gcgccagcat cgccttgtcg ttttcgctga aggcgcccgg ctccggcagc acgccgccga
    10081 gttgctttgc aatcatcgac agcgatcgct gcgcgagatt gccgaggtcg tttgcgagat
    10141 cggcgttgat gcgcgcaaca atggcttcgt ggttgtagct gccgtcctga ccgaacgcga
    10201 cttcgcgcag gaagaaatag cggatctggt cgacaccgta ttggtcggcg agattgaagg
    10261 ggtcgacgac attgccgacc gatttcgaca tcttctcgcc cctgttgaaa aggaagccgt
    10321 gggcaaagac gcgctttggc aacgcgatgc cggccgacat caggaatgcc ggccagtaca
    10381 ccgcgtgaaa gcgaatgatg tccttgccga tgatgtgcag atcggccggc caaaagcgcc
    10441 agttcgcgtc gttctcgtcg gggaagccga cgcccgtgat gtagttggta agcgcgtcga
    10501 cccagacata catcacatga gcggggtcat cggggacctt gacgccccaa tcgaatgtgg
    10561 tgcgcgagat cgagagatcc ttcaggcctc gtttcacgaa gctgacgacc tcgttgcggc
    10621 gcgcgtccgg cccgatgaaa tcgggctggc tttcatagag cgcgaggagc tcgtcctgat
    10681 aggccgacag gcggaagaaa taggtcttct cttcgaccca ctctaccggc gtgccttgcg
    10741 gaccgcgacg cacgtcgtcg tcgccgagaa cggtttcttc ttcggcgtaa taagcttcgt
    10801 cgcgcaccga gtaccagccg gcatatgtgt cgatatagat gtcgccgttc gccgccatcc
    10861 gacgccagat ctcctggctg gaacgatgat gctgcgcttc ggtggtccgg atgtagcggt
    10921 cgaacgagac gttcagtcgt tcatccattg cccggaagcg gcgggagttg cgcgtggcga
    10981 gatctgccgt cgtcaggcct tcgttctccg cggtctgaac catcttcagg ccgtgctcgt
    11041 cggtaccggt caggaagaac acgtccttgc cgtcgagccg ctgaaagcgt gccagcgcgt
    11101 cggtcgctat cgcttcatag gcgtggccga tatgcggcgt gccgttcgga taggcgatcg
    11161 cggtcgtgat gtagaaagta tttccgcggg aggcggcgtt tctcgacgtg gcgggttcgg
    11221 cctggcgcgc agtcgcggcg gacttctgtt ccggcttgtt gggcaaagct tgcttcggct
    11281 ccgaccttgc cctgggcgcc ttccgctttg tcgtcgatgt cttcgctggc tttctggctg
    11341 ctttcttcgc aaccgttttg gcagattttt ttgcggccct ctttgcggtc tttttggccc
    11401 tcttcaggcc acgcttcttt gcggccgcag gcttggcggc agcgcgcttt gcggccgttt
    11461 tggctttttt ctttttcgga gactttttgg ccacgacgaa atcctcgaat gagcctgaac
    11521 gagattgttg ggggcgatga ccctggggac gcctaccgcg tcgcttccgc aagcatcgaa
    11581 aacaccgaga aaaccagcgg ctttcgctcg agattgtagt cctgggtgtc gcgcgcggcg
    11641 cgggcgatct tttcccacac ctcagccagc cgtgcaaggc gcggcaggtt cgtattggca
    11701 ttgccttcgg cgcgcaaccg ctcggcgacc catcgatcga tgctgtcgac gaaggccgcg
    11761 agtgccacgc ggtcgctggt gccgagcgca tctccaagtg catgcaactc gcgggggtcg
    11821 acttgcggca gcgttgccag cagcgctgcg gtacgctgct ggagcttcag cgcatccccg
    11881 cccagcagcg tgatcgcccg cgcaacgctc ccctccgatg cattggcggc ctcgatcagc
    11941 gccggatcgt cggatgcgac gccggtggcc ttcgccgccg cggcgatcac gtcggccgct
    12001 gcaagcggcc gcaacgacag cttgcggcag cgcgactgga ttgtcgccag cacgcgcgcc
    12061 ggtgcatggc tcaccaggaa gaacagcgat tgctgcggcg gctcttccag gatcttcagg
    12121 agggcattgg ccgcgttggg attgagctcg tccagggtgt cgacgatgca gacgcgccag
    12181 ccctcgaccg cggaagtcga accgaaaaac gagatcgtct cacgcgtctc gtccacggtg
    12241 atgaccgtgc gcatcacgcc cctctcgttg agcgttcgct ccagcgtcaa gaggccgcca
    12301 tgggagcccg ctgcaatgtg tcgcgcgctg gcatcgttcg gatcgacaaa cagcgtggtt
    12361 gcccgttgaa cctccgcggc cagcggattg cgatgggtca gcacgaagcg cgccatgcga
    12421 taggcgagcg ttgccttgcc gatcccttgc gcaccgccga tcagccaggc atgcggaatg
    12481 cgcccgctct tgtaggcatt gagcaacgcc gtctccgcct cgcgatgccc gaacagctcg
    12541 aaggtctcgc gcggatgggg agcgctgatc tcggcttcga cccggcgcgc gctcatgcgg
    12601 aactggccac ggtgccggtg ggctcggcaa agaagcggcc gcgcagcgct gcccagacgc
    12661 ttgccgcaac cgtgtccgcg tccgccatcg catcgatcaa cacacagcgc tccggctcgc
    12721 gagctgcgat ctcgtgatag gcgtcccgca gttcctggtg gaagcggatg tcttcggact
    12781 cgaaccggtc gggggcagca ccctcgcggc gggccgcggc tcgcttcaat ccgatctcca
    12841 cgggaacatc gaggatgacg gtcaggtccg gtttgagatc gccgatcgtt acccgctgca
    12901 tggcgttgac gatgccgggc gagacgtgtc caaccttgcc ctgatagacc cgggttgaat
    12961 cgaagaaccg atcgcacagc acccaggtgc cctggttgag cgctggctgg atcacggtgc
    13021 ggacgtggtc gtcgcgggcg gccgcaaaca agagggtctc cgcgtcgggc ccgagcagct
    13081 tgcccattcc cgacagcacg agatgccgga tgatctcggc gccgatcgat ccgcccggct
    13141 ctctggtgac gatcacgtgc agcttcgcgg cttcaagacg ctcggccaac ttcttgattt
    13201 gcgtggattt tcctgatccc tctcccccct cgaaggttat gaaccggccg cgacccggcg
    13261 ggcgcttgat ggcgggctcg atcattgtca gagcttctct gcacccgcgc ggaacatgcc
    13321 gatggcaagc tcgctcgcac catcaatcgc ccgccgtacc gtcgatcccg tgccaatcgc
    13381 ttccgccgca tagacgggcg cttccatggc aacgttgccg ccgcgccaga cgcgcaccac
    13441 gccgatctgc tgaccagcct tgatcggcgc ccgcacgggg ccgctataaa cgaggcgtgc
    13501 gatcagcttg tcattgccct gcttggacac catcaccttg atcggctccg ggctcgagag
    13561 cttgaccgag cggctctctc cgccaaacac ccgggcatag ccgagcggct ggtccgccgc
    13621 gaacagggtg cgcgtttcga aattgcgata accccattcc agcatcttct tggcctcggt
    13681 tgcccgatca tcgggatcct cgagaccgtt gatggccacg atcagccgcg tgccgttctg
    13741 cacggcggag ccgaccatgc cgtagccgcc ttccttggtg tagccggtct tcaacccgtc
    13801 ggcgccttcc atcgagttca acagcgggtt ccggttctgc tggcggatct tgttccaggt
    13861 gaactccttc tcaccgaaca atttgtagaa ctccggatag gtctggatga tgtagcgcgc
    13921 gagttttgcg agctcccgca ccgtcatctt gttgccggga tcgggcagcc cgttggcatt
    13981 gccgaaggtc gatcgcgtga gcccgagttc gcgcgcccgc ttggtcatga actcggatgc
    14041 gaagatgcgc tcgttgccgg ccatgccctc ggcgagcgcg atgcaggcgt cgttgccgct
    14101 ctggatgatc gcgccatgca ggagatcgtc gaccgaaatc ttgctgttca gcaccgcgaa
    14161 catggtcgag ccgccggccg gcgctccgcc ccgccgccag gcgttttcgc tgatccggta
    14221 ctcgtcggtc agcttgacct tgccttcctg gatcgcgtgg aacaccactt ccgctgttac
    14281 gagcttcatc atgctggacg gcgcgcgcag ctcgtccgca ttcttctcaa acagcacgct
    14341 gccgctggaa gcttcgatca ggatcgccgt cggggcgtcg ccgtcgaaac cgccttcttc
    14401 cttcttcgcc ccttgaacgc tctggttcgc cgcatgaagc aggccgcccg aggtcaggcc
    14461 aagtacgacc acgcctgcga tcagccagcg cgaaaggcgc ccgaccgtgg gttggcatct
    14521 gcggagagac aaaaagaaag atggcatcgg tggttgcctc gatgcgggcg ttctaacagt
    14581 tgcaatgatc ccaaacaaca cggcttggcg acgccccctc gattgcgacg ctggcccgag
    14641 tgggcgggtc cttgctgctc ggaatagccg cgacgggcgg taaggtgcgc gcgcggcaga
    14701 ccatgcggag ggccgccgac gaggtcgtcc gacaaggatc ggtgatagcc gccacggcgg
    14761 ctcaatagag cccgcgaccg ctcaggattt cgctgggacc gcggggatca accggcgcgt
    14821 aggccgagac agactgatag tcggcggtat agcgcgcgtc gtcttcgtaa gagactgcgc
    14881 gacgattatc gaggacgcgg ccacccgatc gtgcgcggct cgacgccgac atttcggagg
    14941 tcgcattgat cgaggcatag tcggctgaag tgttgccaag gctatatgga cggtcctggg
    15001 gcagcgggat gtcgccgcgg atcgggcggc cgtaagcgcc ggtctccggc acgaaggggc
    15061 gggccgaggc gacgcggacc agcgagggcg aaggcgccgg cacgcccgtg cgcagggtgg
    15121 caatgagctg gcggtcgtca gagccctcaa gcggggcacg agcgacatat tcgaccctta
    15181 cccgcgcgac accattgccc ttgaattcga ggagttcggc ggctttgttc gagacgtcga
    15241 tgagccggtt gccatgatag gggccccggt cgttgacccg gacaatcagc gactttccgt
    15301 tggagagatt ggtgacccgg gcgtagcagg gcatcggcag ggtcggatgg gcggcggtga
    15361 gcgaatccat gtcgaacacc tcgccattgg cggttagccg gccatggaaa tcgttgccat
    15421 accaggaagc cagcccctcc tcacggtagt tcacgtcctc ctcaggtaca tagacccggc
    15481 ccgccacgac gtagggcttg ccgacgcggt aggtgccgcc gcccttcgga acgggctcgc
    15541 catcggcgac cactctgggg ctggacgaga cgccgtactt cgggtcgatg cggctggcga
    15601 acttgctcga cgaggcgcaa ttgccaagca cgaggcacac acacacagca acggcgccat
    15661 gcgccgctct gatcgccgga tctgagcgca gaaaccccat tcgccccaag caccattcca
    15721 ccggttggct gcctatgtcg gcaaatcccg gtcttttcgc cgatacggtc ccaagctccg
    15781 catcaacgtc taaagatatc gcaaccggaa cacggcggaa atggggagac ctctgcgatg
    15841 gtgaatcgat ggttgcccga tccggtcgtt gattcaccgg gtagaacggg ccttccgaag
    15901 cctccaacct gcccggtttt gaaacacctg ttgcgcagaa tcctcggtcg atcgcgattg
    15961 tgtgctggcg accggaaatc tttttgactg tgcaaggtct ccgccgttct ctgtcgcgca
    16021 ggggcgcaaa ggacgttggc aatgattgag acaatgacgg cagtaatggg tttggtcggc
    16081 gccgctattt tcctggccca tgcgtttgac ggaatccgtt cgcgggtttg acttgggcgg
    16141 aagcgtggat cgcttctttc gaaaaggccg ttcggaaagc tttaataaat tccaacgagc
    16201 atcaaggtcg gagggtgtgt gatgcgaaat caagacctcg tttccgatgg ctttcttgcc
    16261 ctgaccgcgg cgggatttgc ccttctttgc tccagccttc tggctttcgc cttcgtttga
    16321 gccggcggct ccagggctaa tgcgggcaga gcccgcttgg ttcgtacctg ccggaacggc
    16381 ggggccttgt cacagaacaa ctggataggt ccgtgctgcg gcgcaccaaa ttgctctctt
    16441 ctcgcagacc cgtgagtgaa gtggtttcgc cgctgccttg gccgcgaaac gatcggccat
    16501 ttcgatgaag ccattttcct aatcgagcga agatcgccgg aaacaatggc ggatcagcat
    16561 gctcgacaat gatcaacaac ggagtcgatc atgtggatga ttctcagtct gatttatcga
    16621 cactattgcc gggctcgctt gttggagatg cgtaggaacc gcctggcctc tctggtttga
    16681 aagggcctgg cccatgcttg ccgagaaatt cttcctggtc ctggaagcgc tgatcaagac
    16741 gcagcagacc aacgccgacg gcagcgtacg ggtggtcagc acatcgccac atgtgccggt
    16801 caagctgcct gagcggaagt gacgtagcac tggctaacaa caggggacag tgacctgccc
    16861 gaaagcgtgc ttgcgttcgc gcgtatcaac tcaaggtctg cactaccata gctcgccacg
    16921 acgcaagcct cctcctccct cgccgggaat ttgggtagcg gttgtggtag tacagccgcg
    16981 atggttcatg cccctcagtt catcgccgac ctgcagcgct gcgtctgcca gttcgcgcgc
    17041 gtgttttgcg ggaatctttt gcgcggctgg tcgccgccgc gctcgtaatc gctgcagccg
    17101 ccgtccccgc gcatgcaaga acggtccgca tcctgatgga aaatctggtg ctccggccgg
    17161 ccgatacgcg ggccaaagtc ggcgacaccg tcgaatggat caatgacgac atggtcgcac
    17221 acaccgcgac cgcccgaagt ggcgatttcg acgtgatcgt gccgtcgaag cgcacggctt
    17281 cgttcgtgct gctgaaggtc ggcactgtcg agtactattc ccgctttcat cccatcatga
    17341 agggcaggct gaggatcaaa ccatagcctg ccgcttgacc tgaccgattt cggtctcgct
    17401 gtccctccac tcctgcgaca tatatgcgcc gacgtcatca agcgcgggac cggcatccgc
    17461 atttaagccg cgatgtcgta accaaattgc gaagttttcg attcgagcgg gagaccgtcg
    17521 atgaaagcct ttactgccgg attattcgcc gtagtcatcc tctacgctgt cgattcccaa
    17581 tacaatgatg gccgttatgc tcaggtcata gagcaagcag tgaccagcct ggttcgttag
    17641 ggatggccgc ggatcacgcg gagaatctgg ctggcggaat gattggcggc ttcgccaagc
    17701 gcatgtcggg ttcccgatgc aaaagcgcgg gaccgaaacg ccgcgcgttt gttctcgtgc
    17761 tgcaattttg catcaagtta tccacaggct tccgacaacg gatcggccac gaagcgtgaa
    17821 cgtgacacag gaccgttagc ctcgtgtcac tcgtccgtta gccctctgtc acgttgcaaa
    17881 cggccgctga ttgcgttttt tagcacctcc cgggttggaa cctggataga tgaaaccttg
    17941 ttcgagccga acggcggagc aattgggtca gatcaactgc attttccgac tcaggggaaa
    18001 gtgctcgtgt tgtatcgagt attcgaagag gaccccatgc taagccggat ctctcttttg
    18061 cttttaacaa tcgcgagcct gacactcacg gcctacttcg gtgcagaagc ttggcgagct
    18121 tccgacggga ccttgcccag ccctctaacc aagcgctcat ctctttacag ataagcctgt
    18181 tcgaaaattg gaacgccaga cggcatcgtg gcaatgcgcg cgaccgccat tcctcggcat
    18241 tcagcagatg gcagcgttct gcgtcgtgga tccatcctcg taaggcgtct gtaagccaac
    18301 gttgctagta ttgctccgct cggcatttcg atgccgggcc ccaaagcatt tcctccttca
    18361 cccgtcccgc tcccgcgggg cgggtttttt tctttattcg atggaggccg caaaatacgc
    18421 gaaggaaaag ctcaagagcg agcagcgtac agcgctgatg ctggtcgacg gagcggtgtt
    18481 tgaaatttga ggagatcaag cagatatacg aagacactca gaaacagcgc ggaaccgaga
    18541 aaccccgcgc tgtcgttctc atggtgcaga tttgcaccgc ctatccacag gcgaccgtaa
    18601 acggatcggg atcgaggagt gaacacctta cacaaccggg ccgtgtgtgc tgcaatctgg
    18661 taccgtttgt gcgtttatcg gcgccgcgct tctcagagtt caagttcaaa agccgtattg
    18721 gcgcgaaccc aatccgactg accggccttc aatctctttg cttgccattc tgctgtcgcc
    18781 ggatcggcga acaccgccgc caatacccgc ccataatctt caaagctttc gaagccaact
    18841 gtgaaggcta aatttcccac ttcgccgccc atgatggacc agtacgtaac attgccacca
    18901 tgctttctcc aaatggcagc ggcctccttc gcgatctcta aaatcgtggc aagattgctt
    18961 ccaggtctcg gcttcgaaac gtattgaact acaactctcg gcataatact tcccctatgg
    19021 tgatctcgag ccgaatggct cgctataaac ttatcgccga tacttttaag gccgcaacga
    19081 caatcgcgat gcgcgagagc ggcgtgcgcg ggccgctcgt gtgctgcgct gactatcgcc
    19141 gcagccgatg acgaccgttt gaccgcgcca agtgagtgcc gctgaccttt gcgcgttctt
    19201 acttcgttgg ctgcaattcc tgcgaattta tccaaccacc caagtgaaat gcggtatcgg
    19261 cctgaggtgc gtttggccgg ccagggaaca acacggatgc tccaggcatc gacatgctcc
    19321 ccggacaact tgtgggacag ggagtcaccc tcagtcggtg gcggccccgg cgctactccg
    19381 cgagcgttcg cggctgtttt gcgttccgtc gcaggaacca tccccattcg cagcagttag
    19441 gtgatggatc acaaaccctg ccccaccagt gatcaaggca cggtctcgcg accccgcttg
    19501 cggggctgtg ccttcacggt cgcttatgtt gacgggccca agcatcagga acaaacggcg
    19561 gcacgtcggg tctgtcggtt gctccccgga cgctgaaacc ccaaccgatc ccggggagaa
    19621 acctcggccc cagcgagttg gcggccccga ccgccgctcg cattcggtgc cgttttttgc
    19681 gccggattcg caattttacg gcaaatacct acataagttg catgaacaag attctttctc
    19741 ctgagcaaac cgccagattg gagcgacaac gcctggcagc cgaggacggc gcgaaggcac
    19801 ttcaggaagt tgctgagcgt tcggtcgctg tccgcgagaa catggctcgt ctgcgggcat
    19861 tgaggttggc caaggaagcg aaagcggttc ggacgcgaac cgctacagaa aatcagcgcc
    19921 caaattcacg tcgggatcgg cgataggatt tgtcagagcg cgatcgacgc gtcgaccacg
    19981 gccacggttt cgatgctgct cgcgaacggc ggctatcgat tccccgggtg ctggcgggcc
    20041 tgtgcggcgg ctgttgaccg cgccctcggt gccgccataa agaagcgccc ggaggacgct
    20101 ggtttgcaaa gcgttagcaa cgttctgctt tgttctccgg aacggactga aaagcttgga
    20161 tctttcaaag cttggaaggg tggccgagtg gtttaaggca ccggtcttga aaaccggcgt
    20221 gcccgcaagg gtaccgtggg ttcgaatccc accccttccg ccaaaaatga aagaaaaccc
    20281 aataaaaccc gagttttagg cttttggcga
//
LOCUS       DATLJT010000735         5932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3032387,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000735 DATLJT010000000
VERSION     DATLJT010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032387"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VM910_16930"
     CDS             <1..256
                     /locus_tag="VM910_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64247.1"
                     /translation="HRRWRVRQARRPHGHPGARLYPQRHVCGPWECIACPPGVIHGYQ
                     NGSLEPVYFQVMLGRGRPDTMGYVDDELYRRRDAHLGRSC"
     gene            432..1118
                     /locus_tag="VM910_16935"
     CDS             432..1118
                     /locus_tag="VM910_16935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64248.1"
                     /translation="MTIHQGRSGRATKSYSIALEEHYYDSEVKRHASGLDAIAAPATV
                     QRLDDLGELRLKEMDEAGIDLQVLSHTMPGLQKLDAETAVPLARRANDRLNEAVRAHP
                     DRFAAFAALPTANPKAAADELERTVGRLGFKGAMVNGLTNGVFLDDKRFWPIFERAQV
                     LDVPLYMHPAIPHPAVIEAYYNDNVEKHPGILRAGRGFTVETATQGIRLVLSGVFDAH
                     PRLKIIGHLG"
     gene            1496..2455
                     /locus_tag="VM910_16940"
     CDS             1496..2455
                     /locus_tag="VM910_16940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64249.1"
                     /translation="MATKTYIIALEEHYFDPEVKRHTVGQEPTNAPRIVQRLDDVGEL
                     RLREMDEAGIDMQVLSHANPGLQKLDAETAVRLAHGANDRLHEAVRAHPDRFAAFAAI
                     PTPNPKAAADELERTVTKLGFKGALVNGLTNGLFLDDKRFWPIFERAQALDVPLYMHP
                     ALPHPAVVEAYYKDYVEQYPSLLRAAWGFTVETATQGLRLVLSGVFDAYPRLKIILGH
                     MGEGLPFYLWRISMGLARDGRGGRSFRDVFCEHFYITTSGFFSDPALLCCVMEMGIDR
                     ILFSVDYPFVDNPPGTKWTETLPLCAEDKEKLLNGNAKRLLKI"
     gene            complement(2471..4285)
                     /locus_tag="VM910_16945"
     CDS             complement(2471..4285)
                     /locus_tag="VM910_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVI64250.1"
                     /translation="MPETRDGRSIDEWIRVGTLEELKARGMIVVRGGACPLLVVHHRD
                     SVFALDNRCPHLGFPLHRGSIEDGILTCHWHHARFDLVSGGTFDLWADDVPTAPVELR
                     DDGSLWVCRRTRYADGDAHWRNRLREGLQHDIGFVLAKAVLGLGAGRMDHRALVRDGV
                     LFGVRNRDGWGVGLTIATALGNLVPTLPEEERYLALFHGLKRIARDCDGVPPRRDREP
                     LAVGGARPVPLLGRWLRHWTQVRHRDGAERTLLTAIDGGASPSELAALLLTAATDRVF
                     ADGGHALDFINKAFECLDIVGWQEAAAILPTVVPQLVAARGGEELNAWRHPLDLVRLC
                     ETASAALPDLLAIGRAERGRWQSHARLARQLLGDDPEAIIAALAAATRAGATAVDLGR
                     ALAYAAALRVAWFGTANEHSDWETAHHAFTYCNALHQLLKRVTAEQPVELESPELLRG
                     VIHGALRLYLIRFLNVPPARLPGEANDRLGDLPCDDEELRTAFLAALDRQGSVRDAGR
                     LVARYLTLDHPADRLITTLAHAVLREDADFHTYQMLEAGVRQYREWGESDEGRHILIA
                     VARYLAAHSPTERAQLQTAMVARRLSRGEALHETEVES"
     gene            complement(4399..4860)
                     /locus_tag="VM910_16950"
     CDS             complement(4399..4860)
                     /locus_tag="VM910_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64251.1"
                     /translation="MATLDEIGQEKQKVSERLARLDAEREKLGAQLNELEVAERVLTR
                     FGRVETTMRRGRGRPARAAPTPAPAATRGRARAAQPAQTVSLSDATIRAVQAHGEGAT
                     PGEVLSYLAREFGMTVRPNHLGIALQRHRRAGRLETRDSRWFLPQQSAMTG"
     gene            complement(5273..>5932)
                     /locus_tag="VM910_16955"
     CDS             complement(5273..>5932)
                     /locus_tag="VM910_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64252.1"
                     /translation="AEVVRRDPFSIVGQYPNPDLRLCDLAKMRLATGCEVPTPWLCLQ
                     ADLWQAGIDPARLDRIADRSMAENLNALSEGRLEAAQFSSPLSKKPWPREGSPVVRGE
                     HTWPCHLHGFVTPGTGLFATPNRYCPWSGPIQRTQQWIYSRSAPEIAETISSFFPALD
                     RGVLAAALARYQAQSVWGRDPVLPENGFDCLRRYLVSGGFIRRPTHTQPASTIASPCK
                     S"
BASE COUNT         1123 a         1909 c         1850 g         1050 t
ORIGIN      
        1 tcatcggcgc tggcgggtcc ggcaagcacg acgaccccac ggtcatcccg gcgcgcgcct
       61 ttaccctcag cgtcatgtat gtgggccgtg ggagtgtatt gcctgcccac cgggtgtcat
      121 tcacggctac cagaacggaa gcctcgagcc ggtctatttt caggtgatgc tcgggcgcgg
      181 tcgtccggac acgatgggct atgtggacga cgagctctac cggcggcgag acgcacatct
      241 tggccggagc tgctgaggtg cgtgtcactg cggcagtcat ggaagagaag tccggtatgt
      301 tggagctcga caatcgagct cgtgatgccg cgtctcgtcc ggctcatcac cttctacgac
      361 tttgccgcga tcaatccggc ggcggatgcg gccggcggcg cggcgctcaa accggtgctg
      421 tgaatgccgc aatgaccatt caccaaggaa gaagcggaag ggctaccaaa tcctacagca
      481 tcgctttgga ggagcattac tacgattccg aagtgaagcg gcacgccagc ggtctggacg
      541 cgatcgccgc gccggcaacc gtgcagcgtc tcgacgatct gggcgaactg cgcttgaagg
      601 aaatggacga ggccggcatc gacctgcagg tgttgtcgca tacgatgccg gggctccaga
      661 agctcgatgc tgaaaccgcc gtgccattgg cgcgccgcgc caatgaccgc ctcaacgagg
      721 cggtacgcgc ccaccccgac agatttgcgg cctttgcggc actgcccact gccaacccca
      781 aggcagcagc cgacgagctc gaacgcacgg tcggcaggct cggctttaaa ggagcgatgg
      841 tgaacgggct gaccaacggg gttttcctcg acgacaagag gttttggccg atctttgagc
      901 gggctcaggt gcttgacgta ccgctctaca tgcacccggc tatcccgcat cctgcggtga
      961 tcgaggctta ctacaacgat aacgtcgaaa aacatccggg gatcttgcgc gccggcaggg
     1021 gctttaccgt cgagaccgcg acgcagggca ttcgcctcgt gctgagcggc gtgtttgacg
     1081 cccatccgcg tctcaaaatc atcgggcatt tggggtgaag ggctgccctt ctacctctgg
     1141 cgcatcagcc acgggttgcg cggcagcgac ggcggcaaat ctttccgaga cacctttcgc
     1201 gagcactttc atgtcaccgc cagcgggttc tttcccgatc cggctttgct gtgctgcgtg
     1261 atggaaatgg ggatcgaccg gatcctgttt tccgacgact atccgttcgt cgacaatccg
     1321 ccagcgacga aatggactga accctgcccc tgtgcgccga ggacaaggag aaattgttga
     1381 acggcaacgc caaacggttg ctcaagcttt aggggcgccc cggcctgacg catgacaggc
     1441 cgacccctgg ctgttagaaa tccgcaaccc gaccctgcat ccgtggagga gcgcaatggc
     1501 cacgaagacc tacatcattg ctctggagga gcattatttc gaccctgagg tgaagcgtca
     1561 caccgtcggc caggaaccga ccaatgcgcc gcggatcgtc cagcggctcg acgatgtcgg
     1621 cgaattgcgc ttgagagaaa tggacgaggc cggcatcgac atgcaagtgc tgtcgcatgc
     1681 caaccccggc ttgcagaagc ttgacgcgga gaccgccgtc cgcctggcgc acggcgccaa
     1741 tgaccggctc cacgaggcgg tccgcgctca tcccgaccgg ttcgcagcct ttgctgcaat
     1801 cccgaccccg aacccgaaag cggcggccga cgagctcgaa cgcacggtca ccaaattggg
     1861 ctttaagggc gcgctggtga acggcctgac caacgggctc tttctcgacg acaagcggtt
     1921 ctggccgatc ttcgagcggg cgcaagcgct cgacgtgccg ctctatatgc atccggcgct
     1981 gccccacccc gccgtcgtcg aggcttacta caaggactac gtggagcagt atccctcgtt
     2041 gttgcgcgcc gcctggggct tcaccgtcga aaccgcgacg caaggcctgc gtctcgtgct
     2101 gagcggcgtc ttcgacgcct atccgcgtct caagatcatc ctcggccaca tgggtgaagg
     2161 actacccttt tatctctggc gcatcagcat gggactagcg cgtgacggta ggggcggcag
     2221 gagcttccgc gatgtattct gcgagcactt ctacatcacc accagcggct tcttttccga
     2281 cccggcgttg ctgtgctgcg tgatggaaat ggggatcgac cgaatcctgt tctcggtcga
     2341 ctatccgttt gtcgacaatc cgccgggaac aaagtggacg gaaaccctgc cactgtgcgc
     2401 cgaggacaag gaaaagttgc tcaacggtaa cgccaaacgg ctgctaaaga tttagagacc
     2461 tggatgccga ttaggactca acctcggtct cgtgcagcgc ctcgccccgg ctgaggcgac
     2521 gcgccaccat cgcagtctga agctgcgccc gctcggtcgg cgaatgggcg gcgagatagc
     2581 gagcgaccgc aatcaggatg tggcgtcctt cgtcgctctc gccccattcg cggtattgcc
     2641 gcacgccggc ttcgagcatc tgataggtgt ggaagtcagc gtcctcgcgc aggactgcgt
     2701 gcgccagcgt cgtgatcaac ctatcggcag gatgatcgag cgtcagatag cgggcgacga
     2761 ggcggccggc atcacgcact gatccctgtc gatcgagcgc cgcgagaaat gccgtgcgca
     2821 actcctcgtc atcgcatggc aggtcgccga gccggtcgtt ggcctcaccg ggtagtcgtg
     2881 caggcggcac gttcaagaaa cggattagat aaagtcgcag cgccccgtga atcacgccgc
     2941 gcagcagctc ggggctctcc aactccaccg gctgttcggc ggtgacacgc ttcaggagct
     3001 ggtgcaacgc gttgcaatag gtgaaagcgt ggtgcgccgt ctcccagtcg gaatgctcgt
     3061 tcgcggtgcc gaaccaggcg acacgcaacg ccgccgcata agcgagcgcg cggccgagat
     3121 cgaccgcggt cgcgccggca cgcgtcgccg ccgcgagtgc agcgatgatc gcttcgggat
     3181 catcgccgag cagctgccgc gccaatctgg catggctttg ccaccggccc cgctcggcgc
     3241 ggccgattgc cagcaaatcg ggcaacgcgg ccgaagccgt ctcgcacagc cgcaccaagt
     3301 cgagcggatg tcgccacgca ttcagttcct cgccgccgcg cgcggcgacc agctgaggaa
     3361 cgaccgtcgg cagtatcgcc gctgcctcct gccagccaac aatgtcgagg cattcgaatg
     3421 ccttgtttat gaaatccaga gcgtgaccgc catcggcaaa aacacggtcg gtggcggcag
     3481 tcagcagcag cgctgctagc tcgctcggcg atgcgccgcc gtctatggct gtcagcagag
     3541 tgcgctcggc gccgtcgcgg tggcgcacct gggtccaatg ccggagccaa cggccgagca
     3601 acggcaccgg gcgcgcgccg ccgactgcaa gcggctcgcg gtcccggcgc ggcggcaccc
     3661 cgtcgcagtc cctcgctatt ctcttgagac cgtgaaagag ggcgaggtac ctctcctcct
     3721 ccggcaaggt cggaacgagg ttgccgagcg cggtggcgat ggtcaggccg acaccccatc
     3781 cgtcacgatt gcggacaccg aacagtacgc cgtcgcgaac caacgcgcga tgatccattc
     3841 gaccggcgcc gagacccagc accgccttgg cgaggacgaa accgatatcg tgctgcaagc
     3901 cctcgcgcag gcgattgcgc caatgggcgt cgccatcggc ataccgcgtt cgccggcaaa
     3961 cccatagaga accgtcgtcc cggagctcga cgggggcggt cgggacatcg tccgcccaga
     4021 gatcgaaggt cccgccgctg acgaggtcaa agcgggcatg atgccaatgg caggtcagaa
     4081 tcccgtcctc gatggagccc cgatgaagcg gaaaaccaag atgagggcac ctgttgtcga
     4141 gcgcaaagac actgtcccgg tggtggacga cgagcaatgg acaagcgccg ccgcggacaa
     4201 cgatcatccc ccgcgccttt agctcctcca gcgtcccgac gcggatccac tcatcgatcg
     4261 aacgcccgtc acgcgtctcg ggcatcgtgc ctcgctccga cactactggt gacggcactt
     4321 gcggcagcgt cggagcgttt cgcggcagct ccgatgcagc gccgaggatg cagccgcctc
     4381 acattcctct atttgccgtc agcctgtcat cgctgattgc tgcggcagaa accaacgtga
     4441 atcacgagtc tcgagccgcc cagcgcgccg gtggcgctgc agcgcaatac cgaggtggtt
     4501 cggcctgacc gtcatgccga attcgcgagc gaggtaagag agaacctcac cgggggtggc
     4561 gccttcgcca tgcgcctgga cggcgcggat agtggcatcg ctcagcgata cggtctgtgc
     4621 cggttgcgca gcccgggctc tgccgcgtgt ggcggccggc gccggcgtag gcgcggctcg
     4681 cgcgggtcgt cctcgtccgc gtctcatcgt cgtctcgact cttccgaacc gtgtcaacac
     4741 gcgctcggcg acttccagtt cattgagttg ggcacccagc ttttcccgtt cggcgtcgag
     4801 ccgcgcgagg cgctcggaga ccttctgctt ctcttgtcca atctcgtcca acgtagccat
     4861 ctgctctctc cctcttcccc catgactagt gttgtcacac tgcaccgtta ttctgcaata
     4921 aatggaggac cgccgcaccg aaaaaagatg gcgcttgaga aattgttgcc gttttatggc
     4981 tgtgccgcga accctcgcaa tgcggcaagg cgcagcgtgc tcgagttcgg aggcacacac
     5041 gatccgcagt ttgcttgagt attctgccaa cgatctgaac gtcgaccgca gaaaatgccc
     5101 aaaggcgtca gacgagcaat gccgtccgat gcgccccgcc tggctgaccg cgtagtagag
     5161 gccgcgcggc agataaatcc cgaatcgcct ttgcggtgat aaatccgaat cgcctttgat
     5221 gccggattga ctatcagcac gcgcttcggg ttcgtacgca aatcctattc ggtcaggact
     5281 tgcagggcga ggcgattgtc gacgcaggct gcgtatgcgt cggccgacgg atgaacccgc
     5341 ccgaaacgag gtatcgccgc agacaatcaa agccgttctc gggaagaacc ggatcgcgtc
     5401 cccacaccga ctgtgcctgg tagcgagcga gtgcggcggc cagcacaccg cgatcgagcg
     5461 ctggaaagaa cgatgagatc gtctcggcga tctctggcgc ggaccggcta tagatccact
     5521 gctgtgtccg ctggatcggc ccggaccatg gacaataacg gttcggcgtc gcgaacaaac
     5581 cggtcccggg tgttacgaaa ccgtgcaagt ggcacggcca cgtgtgctcg ccgcgtacca
     5641 caggtgaccc ttcccgaggc caaggcttct tcgacaacgg gctcgaaaac tgcgctgcct
     5701 cgagccgtcc ctcgctcaag gcattgaggt tttcagccat gctgcgatcg gcgatgcgat
     5761 cgagacgcgc cgggtcgata cctgcctgcc acagatccgc ctgcagacat agccacggtg
     5821 ttggcacctc gcagccggtg gcaaggcgca ttttggcaag gtcgcagagc cggagatccg
     5881 gattggggta ttggccgacg atcgagaacg gatcgcggcg gacgacctct gc
//
LOCUS       DATLJT010000736         1940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2343017,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000736 DATLJT010000000
VERSION     DATLJT010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343017"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VM910_16960"
     CDS             complement(<1..858)
                     /locus_tag="VM910_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64253.1"
                     /translation="MDQGKRRTPFRSSFEAEHIDLGNEQPLSVDGENSGLVDRRRVSV
                     QWFSATILAGLCGAALMGGAVFAALDGESNFATVPERVEAALRTVAAGNSERATNATR
                     KNDRLPPPGEANAARQIIRVSTTTRGSGNREIVRVRPFIRVAGNLSLTVSELSAGIPP
                     FNAQKMLAQSAGGAGGGEESADAEPDAEVSFLTRDLVTVLPRAKIAAVLPVDEILPRV
                     LEAANWDGGMTAKRPLISDSIPLTAYAPERNSDPYAGFEPRIVPENVTLLPKTTAQVT
                     GGSAWTERTF"
     gene            1691..>1940
                     /locus_tag="VM910_16965"
     rRNA            1691..>1940
                     /locus_tag="VM910_16965"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          342 a          591 c          655 g          352 t
ORIGIN      
        1 aaacgtgcgc tcggtccagg cgctgccgcc ggtcacctgc gccgtggtct ttggcaggag
       61 cgtgacgttc tccggcacga tgcggggctc gaagccggca tagggatcgc tgttgcgttc
      121 cggagcatag gcggtgagcg ggatcgagtc gctgatgagc gggcgcttcg cggtcatgcc
      181 gccgtcccaa ttcgcagcct caagcacgcg cgggagaatc tcatcgaccg ggagcacggc
      241 ggcgatcttc gcgcgcggca gcacggtgac cagatcgcgc gtaagaaacg atacttccgc
      301 gtcaggctcg gcatcggcgg attcctcccc gccgcccgcg ccgcccgccg attgcgcgag
      361 catcttctgc gcgttgaacg gaggaatgcc ggcggagagc tccgataccg tcagcgaaag
      421 atttccggcg acgcggatga acgggcgcac gcgcacgatc tcgcgattgc cgctgccgcg
      481 ggtggtggtg gaaacccgaa tgatctggcg cgccgcgttg gcttcgccgg ggggcggcag
      541 ccggtcgttc ttgcgggtgg cattggtcgc gcgttcggag ttgccggcag cgacggtgcg
      601 cagcgctgct tccacccgct ccgggacggt cgcaaaattg ctttcgccat cgagcgctgc
      661 aaaaacggcg ccgcccatca aggccgcgcc gcacagacca gccaggatgg tggcgctgaa
      721 ccattgcacc gacacgcgcc ggcgatcgac caggccgctg ttctcgccat cgacggacag
      781 cggctgctca ttgccgagat cgatgtgctc ggcctcgaag ctcgaacgga atggagttcg
      841 ccgtttgccc tggtccaagc cccacgtccc ctcacgcccg tgtcgtgcgg gcagcccgca
      901 cgacacgaaa ggtgccaaac gcgaccggga cagcggcgtt gacgtggcgg ctcaggacgg
      961 agtccgctcc acggttgcgt caacgaccgt gtccgtagtc ttgcggctca tcgttgggtg
     1021 gcgccatatg tcggcttgcc cgacatcggg cgcgagttcc cccgaagaac gacacctccc
     1081 actgcgtcgg ccatgggggc ggacgaatgt ggctgcagtg cggcgcgggg aaccctttgc
     1141 agcctgtcta agctcccgaa attgcttgcg aagatgactg tggctctccg gcatcaaggc
     1201 acccgatttt ctggggccgg cgggtgttga cttttgcgtc tcgcgacgcc tatataaccg
     1261 gtcaattcgg ctcgccgccg cctcggcgcg acggcgtgtc ttcgaagctc ctcactgata
     1321 ttcggtgatt cggctccgat cgcgatcgga agccacgagt ttcggcggct tttgacttcg
     1381 acggctctcc gtttggttgg agagtggggc tggcttgggt cccgggctgt ttgacaggtg
     1441 aatcggaaga aagagaaacg tggacggcgg agtccctgcg gactgcgatt ccaccgaggg
     1501 ccggcttagc agcgggccaa ccgggggaac agcggtcgga cgagacttcg gcggtcgacg
     1561 tttcaggtca acacccctgt cgtgagagcg caagtgcagg tgcggcgtca aaccgcacag
     1621 gtgcgtcgcg cccacgcgac gggagtggga cctcgtcaaa cgttgtgatc tgccgggata
     1681 agttctcatc caacttgaga gtttgatcct ggctcagagc gaacgctggc ggcaggccta
     1741 acacatgcaa gtcgagcgcc cctccttcag aggacagtag tcaaactgtc ttctgatgga
     1801 ggggagcggc agacgggaga gtaacacgtg ggaacgtacc ctttggttcg gaacaaccca
     1861 gggaaacttg ggctaatacc ggataagccc ttacggggaa agatttatcg ccgaaggatc
     1921 ggcccgcgtc tgattagcta
//
LOCUS       DATLJT010000737         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1194923,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000737 DATLJT010000000
VERSION     DATLJT010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194923"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VM910_16970"
     CDS             <1..378
                     /locus_tag="VM910_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI64254.1"
                     /translation="QDTILVQINPVERPGSPRSARDILNRLNEVSFNAVLLKELRMSL
                     LRQIAQPDNSENAKWADMRIHRISSDVMVELGYSSKLNAEWEFLCMLRDEGRRAGDIF
                     LSTHHDDLGRRSTFDLDELLKGV"
     gene            383..1837
                     /locus_tag="VM910_16975"
     CDS             383..1837
                     /locus_tag="VM910_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HVI64255.1"
                     /translation="MGLIGILLGLALLIWLALRGWSVLLLAPAAALLAAAFALEPLLA
                     NWTQTFMGSAAAFLAQFFPLFLLGALFGKLMDDSGCVSAIAGFMTERLGERRAVLAVV
                     LAGALVTYGGVSLFVAFFVLVPMAQALFRAASIPRRLMPAAITLGTSTFTMSALPGTP
                     AIQNAIPMPFFGTTPFAAPGLGIVASAIMLGFGLWWLGRAERTARRAGEGYGAPDGAS
                     VDTAADDPVVRELATTAREFDPAEIHRGQHGDTKPPTARAFLPLVVVVSVNLLMSLVL
                     LPRLDFSFLAEERWGSTSLSAVAGVWAVVAALASAIATLIAINYRYFPALRASMDAGA
                     NASVLPAVTIASLVGFGAVVAALPAFAAVRDWVLSMEGGPLVSLAVATNVLAALTGSA
                     SGGLTIALDALGNAYMELAAGKDIEPALMHRVAVIGSGTLDSLPHNGAVVTLLALCGL
                     THRESYFDIVMVGIVGAIIALVAVIALGSAVGSF"
     gene            1908..2930
                     /locus_tag="VM910_16980"
     CDS             1908..2930
                     /locus_tag="VM910_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVI64256.1"
                     /translation="MTSRGALTMLGLCTAVLVFAGLYFARSILAPVAFSLFVVAIVWP
                     LQRALQIRTPRLLALVAALVVTLLVVTVLGYLIVWAFGTVGRWLIDNALRFQALYIQS
                     RDWLEEHGILVTSLLVENFNASWLIRAVQEIGLRLHGLVTFVVITFVFTALGLLEVDI
                     AGKNIERLGNEEVAKTILQASTQIADKFQKYMLVRSAMSVLTGLVVWVFTLLAGLELA
                     TAWGVIAFVLNYIPFIGPLVATVFPTLFALAQFGSWQLAIVVFLGLNLIQFLIGSYLE
                     PRIAGAALSVSPFLVLFAVFFWAFLWGIPGAFIGVPIVIAGLTLCEQRESTRWIATLL
                     SGRERR"
     gene            complement(3046..>3270)
                     /locus_tag="VM910_16985"
     CDS             complement(3046..>3270)
                     /locus_tag="VM910_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI64257.1"
                     /translation="PLAGRAVLMHGHRHIDWIGECGGIPILSAPSPVMEATDDVATCF
                     YIHTLAVGPDRRLRLLRPQRVTVQGEPTSA"
BASE COUNT          491 a         1058 c         1011 g          710 t
ORIGIN      
        1 caggatacga ttctcgttca gatcaatccg gtcgaacggc cggggtcgcc gcgctctgcc
       61 cgcgacattc tcaaccggct caacgaggtc tcgttcaatg ccgtgctgct gaaagagctg
      121 cgcatgagtt tgctgcgcca aatcgcacag ccagataatt ccgagaacgc caaatgggca
      181 gacatgcgca tccaccgcat ctccagcgac gtgatggtag agctcggcta ttcttcgaag
      241 ctcaacgccg agtgggaatt cctgtgcatg ttgcgcgacg aggggcggcg cgcgggtgac
      301 atattcctga gcacccatca cgacgatctg ggccgacgct ccaccttcga cctcgacgag
      361 ttactcaagg gtgtatgaca ccatgggtct catcgggatc ctactcggat tggcgctgct
      421 catttggctc gcgctgcgcg gctggagcgt gctccttctc gctccagccg ccgcgctgct
      481 tgccgccgcg ttcgcgctgg agccgctgct cgccaactgg acacaaacct tcatgggcag
      541 tgctgcggcc ttcctggccc agttcttccc cttgtttctg cttggcgccc tgttcggcaa
      601 gctcatggac gatagcggct gcgtgtccgc catcgcgggg ttcatgaccg aacggctggg
      661 tgagcgccgc gccgtgctcg cggtcgtgct cgcaggtgcg ctcgtaacct atggcggcgt
      721 cagcctgttc gttgctttct ttgtgctcgt ccccatggcg caggcgttgt ttcgagcggc
      781 gtccattccg cgccgcttga tgcctgcggc gatcacgctg ggtacctcaa ccttcacgat
      841 gtcagccctg ccgggaacgc cggcgattca gaatgcgatc ccgatgccat ttttcggcac
      901 cacgccattc gccgcgcccg gtctggggat cgtcgcatcc gccatcatgc tgggattcgg
      961 cctgtggtgg ttgggtcggg cggagcgcac cgctcgccgc gccggagagg gctacggcgc
     1021 tcctgacggc gcgtcggtcg acactgcagc ggacgatccg gtcgtgcgcg aactcgccac
     1081 caccgcccgc gagttcgatc ctgccgaaat ccatcgcggc cagcacggcg ataccaaacc
     1141 gcccaccgct cgggcgttcc tgccgctggt cgtcgtggtg agtgtcaacc tgctgatgtc
     1201 gctggtcctg ctaccgcgcc tcgacttttc attcctagcg gaggaacgtt ggggctccac
     1261 ctcgctctcg gcggtcgcag gcgtttgggc ggtggtggct gcgctggcgt cggcgatcgc
     1321 cacgctcatc gccatcaatt atcgctactt tccggcgttg cgcgcgagca tggacgcggg
     1381 cgccaatgcc tcggtattgc cggccgtcac catcgcaagt ctcgtgggct tcggcgcggt
     1441 ggtggcggca ctccccgctt tcgcagcggt ccgcgattgg gtgctgtcca tggagggggg
     1501 acccctcgtg tcgctcgccg tcgccaccaa tgtgctggcg gcgctcaccg gttcggcctc
     1561 gggcggtctc accatcgcgc ttgatgcgct gggcaacgcg tacatggagc ttgcggccgg
     1621 gaaagacatc gagcccgcgc tcatgcatcg tgttgccgtg atcggctccg gaacgctcga
     1681 cagcctgccg cacaacggcg ccgtggtgac gctgctcgca ttgtgcggct tgacgcatcg
     1741 cgagagctat ttcgacatcg tcatggtcgg aatcgtcggc gcgatcatcg cgctcgtcgc
     1801 ggtgatcgcg ctgggctcgg ccgttggctc tttctagacg tcttgcattc gtcggcatcg
     1861 gccatgaaag tccgccaacg atacaaactt caggggacga gggttgcatg accagcagag
     1921 gcgcactgac catgctgggc ctctgcaccg cggttctggt ctttgccggc ctttacttcg
     1981 cgcgctcgat tctcgcgccg gtcgcgttct cgctgttcgt cgtcgctatc gtctggccgc
     2041 tgcagcgtgc actccagatc aggactccga ggctgcttgc gcttgttgcc gcgctagtgg
     2101 tgaccctgct ggtcgtgacc gtgctcggct acctgatcgt gtgggcgttc ggaacagtcg
     2161 ggcgctggct gatcgacaac gcgctgcgat ttcaagcact ctatatccag tcaagagact
     2221 ggctcgaaga gcacggcatc ttggtgacga gcctgctggt tgagaatttc aacgcgagct
     2281 ggctcattcg cgcggtgcag gagatcggcc tcaggctcca cggtctggtg acgttcgtcg
     2341 tcatcacctt cgtcttcacc gctctcggcc tgctcgaggt ggacatcgcc gggaagaaca
     2401 tcgaacgcct cgggaacgag gaggtggcga aaacaatcct gcaagcgagc acgcaaatcg
     2461 ccgacaagtt ccaaaaatac atgctggtcc gcagcgccat gagcgtgctg accggcctgg
     2521 tggtctgggt ctttaccctc ctcgccggtc tggagctcgc gacggcatgg ggcgtgattg
     2581 cgttcgtcct caactacatt ccgtttatcg gccctctggt cgccaccgtg tttccgaccc
     2641 ttttcgcgct ggcgcagttc gggtcctggc aattggccat cgtcgttttt ttgggcctca
     2701 acctcatcca gttcctgatc ggaagctacc tcgaacctcg catcgccggc gccgccctct
     2761 ccgtctcgcc gttcctggtt ctattcgcgg ttttcttctg ggcctttctc tggggaatcc
     2821 ccggagcatt catcggcgtg cccattgtga ttgcgggtct cacgctgtgc gaacagcgag
     2881 agtcgacgcg atggatcgcg acgcttctgt cggggcgcga gcggcgctag taccccaatt
     2941 ccgaggttcg caatcccttg cggcacacct ttgtgcgaac ggttttggat ttgaagttcc
     3001 tgcgcgaact cgctgcgtat tcgtgggaac ttctaatcca ccgcactagg cggaggtcgg
     3061 ttctccctgc acggtgaccc gctgcggccg cagcaatctg agccgtcgat ccggccccac
     3121 agccaacgtg tggatgtaga agcacgtggc cacatcgtcg gttgcttcca tgaccggcga
     3181 cggcgcggaa aggatgggga tgccgccaca ttccccgatc caatcgatgt gccgatgacc
     3241 atgcatcagc accgcccggc ccgcgagcgg
//
LOCUS       DATLJT010000738         4249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1654693,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000738 DATLJT010000000
VERSION     DATLJT010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654693"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1099
                     /locus_tag="VM910_16990"
     CDS             119..1099
                     /locus_tag="VM910_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64258.1"
                     /translation="MKRREFIALIGGAAAAWPRPPRAQQETTRVIGVLGSATAREWAP
                     SVAAFLRGLSEAGFAEGRDVAIEYRWAENQYDRLPSLSAELIHRQVSVIAALTTPSAI
                     AAKAATTTIPIVFTTIADPVQIGLVASLNHPGGNLTGVTFLNVEIVPKMLELLHEVVP
                     TATTIAALVNPTNPNADIWSTGLQGAARTLGLELNVLTASTERDIDKVFAALIPLRVG
                     GLVIVNDVFLITRQEQLAALALHHRVPTIFQTRASVVAGGLMSYAGSASDAYRQAGVY
                     TGRVLKGEKPADLPVVQSTKVELILNLKTAKALGITVPLPLSGRADEIIE"
     gene            1406..2041
                     /locus_tag="VM910_16995"
     CDS             1406..2041
                     /locus_tag="VM910_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64259.1"
                     /translation="MDQASLTSFMAVILTRGTERILLVLVGALAIYLGYSLFLRMPNA
                     VRGKHDGEGKIELPGGVSIFLTRVGPGIFFALFGIAVIGYSVTRPVNLDFPAGTTVAS
                     AGGLHFSGIAQSEPAKILTQAVIGGPEPDAVVPRLNGIFKKMQSQMNAVEAEELAQII
                     RAAKIAVILGRWKPEWGDRVVFERWVREHVNQDPPPTLVPGAGLLYYGISP"
     gene            2053..3366
                     /locus_tag="VM910_17000"
     CDS             2053..3366
                     /locus_tag="VM910_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64260.1"
                     /translation="MSTFRAFWCVFTLSWALSGAVRAGNLDNIYDDQELANLQPRYER
                     GWRSNYNDFFLPVFTAAERARFAQVRIRFEPHVSDREPFGFYSGGDQIVASTASLKFL
                     FEITLAQRWLEMNGFDTATVNQYLTMVRYWDAKRDRPPKPLETLCIPPESSIDPKIVE
                     SAEGIFNDAAYFVLLHEYGHALYRHPGNLMVPPAESRANEEAADLFALSVFARRESPP
                     AGVPWFFFVTTQLLEFSPDAGSASQRASLAARTHPVSPERLRAVARQIVAQTNAYAKG
                     FKPGAHNSPLTLALEISRLANLLGDPSVQQASDRIGKTVSPIDLAPRPKGRNLSAPCD
                     GRVPSRLPYDGTLRGKLLASGVEFNVDMVLAQNNEKVTGSFSYGNGVGRVFGKVQDGR
                     LIYVWTLGADTGGGVMTAQNNSYRGSWGYGTSPTGGGSFELTKQP"
     gene            3581..>4249
                     /locus_tag="VM910_17005"
     CDS             3581..>4249
                     /locus_tag="VM910_17005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64261.1"
                     /translation="MKLPRRKFLHLAAGAAALPAVSRAARAQAYPSRPITMMVPFPPG
                     GLTDVVGRLIAEGMRTALGQTIIIENVGGATGSIGTGRVARAAPDGYTITVGIWNTHV
                     ANGITYALQYDVVSDFEPIALLADAPLLFLGKKDLPANSLKELIGWLKENPDKASMGS
                     TGVGGPGHLLALLLQEKTGTRFNLVPYRGVNPSVQDLIAGQIDMTISNTATAMPHVRA
                     GRVKA"
BASE COUNT          825 a         1286 c         1291 g          847 t
ORIGIN      
        1 cggaccccat cagggccagc ggtccatccg gactgcgtga acaggccgga cacatgactg
       61 caagcgactt ctgtgcggca cgctcaattt tctcttgcca ggcgggggcc atccacacat
      121 gaagcggcgc gagttcatcg cgcttatcgg cggcgcagca gccgcgtggc cgcgccctcc
      181 acgggcgcaa caggagacga cgcgggtgat tggggtcctt ggcagtgcaa cggctagaga
      241 gtgggcgccg tcggtagccg cattccttcg aggcttgagc gaggctggct ttgctgaggg
      301 ccgggacgtc gcaatcgaat atcgttgggc cgaaaatcaa tacgatcggt tgccgtccct
      361 gtcggccgaa ttgattcacc gccaagtgtc cgtgatcgcc gccttgacca ccccttctgc
      421 aatcgcagca aaggcagcca ctacaaccat acctatcgtt ttcactacca tcgcggatcc
      481 ggtgcagatc ggtctcgtcg cgagcctgaa ccaccccggc ggcaatctca caggtgtgac
      541 cttcctaaac gttgagattg tgccgaagat gttagagcta ctgcatgagg tggttcctac
      601 ggctaccacg atcgccgcac tcgttaaccc gacgaatccc aatgccgata tctggtctac
      661 aggtctgcag ggcgctgcgc gcacgctagg gctcgaactc aacgtcctga cggcaagcac
      721 cgagcgcgac attgataagg tcttcgccgc cctaatccca ctgcgggttg gcgggctcgt
      781 catagtcaac gatgtcttcc tcattacacg ccaggaacag ctcgctgcac tggcgcttca
      841 ccacagggtg ccgacgatct tccaaacgcg cgcatctgtc gtagctggcg gtctgatgag
      901 ctacgcaggc agtgcctcgg atgcctatcg tcaagctggc gtatataccg gccgtgtcct
      961 taaaggcgag aagcctgctg acctcccggt cgtgcagtcc acgaaagtcg agctgatcct
     1021 caacctcaag accgccaagg cgcttggcat caccgtcccg ctacccctgt ccggccgcgc
     1081 cgacgagata attgaatgaa tgatcaaacg ccgcgagcat cacgctgttc ggcggcgcgg
     1141 cggcggcgtg gccgctcgcg gcgagcgcgc agcaggcggg caaggtgccg accactcgca
     1201 ctcctgggca gcgcggccgt cgcctggccg ctcgcggagc agctggcggt accggtgatt
     1261 gggctcgtga ggaccatacg cctgaagatt cggcgccgtg tgcctgcgaa aataccgtta
     1321 cgacctaaat gatgcccatt tatattattg tacttgaggt atgctacgct tgagataagt
     1381 cccttttccc cggggggcgc cggtcatgga tcaggcctct ttgacaagct tcatggccgt
     1441 cattctgacg cgcgggaccg agcgcatact tctcgtgctg gtcggcgctc tcgcgatcta
     1501 tctcggctac agcctttttc tccgaatgcc gaacgccgtc cgtggcaagc acgacggcga
     1561 aggcaaaatc gaattgcctg gcggcgtatc gatctttctc acgcgcgtcg gcccgggaat
     1621 tttcttcgca ttgttcggca ttgcggtgat tggctattcg gtgacgcggc cggtcaatct
     1681 cgatttcccg gcaggaacga ccgtcgcctc cgcaggcggc ctgcattttt cggggatcgc
     1741 tcagagcgag cctgcgaaaa ttctgaccca ggccgtcatc ggcgggcccg aacctgatgc
     1801 tgtcgtccct aggctcaacg gcatttttaa aaaaatgcag agccagatga acgccgtgga
     1861 ggcggaagag ctggcgcaga tcatccgcgc cgcgaaaatc gccgtcatcc tcgggcgttg
     1921 gaagccggaa tggggtgacc gtgtagtgtt cgaacggtgg gtacgggaac acgtcaacca
     1981 agatccaccg ccgacactcg taccgggcgc tgggttgctc tactacggca tatcgccgtg
     2041 aggacggcag ctatgagcac gttccgtgct ttctggtgcg tctttacgtt gagttgggcg
     2101 ctgtcgggcg ccgtgcgcgc gggcaacctc gacaatattt acgacgacca ggagctggcc
     2161 aatttgcagc cgcgctacga acgcggctgg cgcagcaact acaacgactt ctttttacca
     2221 gtctttactg ctgcggagcg cgcgcgcttt gcgcaagttc gaattcgttt cgagcctcat
     2281 gtgtccgatc gcgagccctt cggattctac tccgggggtg atcagatcgt tgcctcgact
     2341 gcgtcgctga aatttctctt cgaaattaca cttgcccaaa gatggctgga aatgaatggc
     2401 ttcgacaccg ccaccgtcaa tcaatatctg acgatggtgc ggtattggga cgcaaaacgg
     2461 gaccgtccac caaaaccact ggaaacgctc tgcatcccgc ccgaatcttc gattgatccc
     2521 aaaatcgtcg aaagtgccga gggcatattc aatgatgccg cgtacttcgt gctgctgcac
     2581 gaatatggtc acgcgcttta tcgtcatccg ggcaatctca tggtgccgcc ggcggaatcg
     2641 agggcgaacg aagaagcagc cgatcttttt gcactcagcg tttttgctcg cagggaaagc
     2701 cctccggcgg gtgtcccttg gtttttcttc gtgacgactc agctgcttga attcagtccg
     2761 gacgcgggga gcgcctcgca gcgggcttca ctggccgcgc gcacacatcc ggtctcgcct
     2821 gagcgcctgc gggcagttgc ccgccaaatc gtcgcgcaga cgaacgccta tgccaagggg
     2881 ttcaagccgg gcgcgcacaa ttcgccgttg acgcttgccc tggagatatc tcggcttgcc
     2941 aacttgctcg gcgatccgag tgtgcagcag gcttctgacc gtattggcaa gacggtcagt
     3001 ccgattgatc ttgctccgcg tcccaagggc cggaacctgt ctgcgccgtg cgacggcagg
     3061 gtgccgtcca gacttccgta tgacggtacg ttgcgaggaa aattgttggc cagtggcgtt
     3121 gaattcaatg tcgacatggt gctggcgcaa aacaacgaaa aagtgaccgg gtcattcagc
     3181 tacgggaacg gcgtcggacg tgtcttcggt aaggtccagg acggcaggct catttacgtg
     3241 tggacgttgg gcgcggacac cggcggcggt gtcatgactg cacaaaacaa ctcctaccgc
     3301 ggcagctggg gctacgggac ctcgccgacg ggtggcggat cgttcgaact gacgaaacag
     3361 ccgtgacctt gctcgaattc ggtgtcgacg gtgacgtagg cgtgcttcgt ctgaagttcc
     3421 atccaagcgc cagctcaaat tgggctgtca ctcctcggta agctggcatg gcggccttga
     3481 acttcaagac tgcgaaggcg cttggcctcg aaatcccgcc gtcagtactc gcccgcgccg
     3541 acgaggtgat ggaatgatgt gaagcaagag ggaggttcaa atgaaacttc cccgccgcaa
     3601 attcttgcat ctggccgccg gcgctgccgc gctcccggcc gtgtcgcgcg ccgcacgggc
     3661 gcaagcatat ccatcacgac caatcacgat gatggtgccg tttccgcccg gcgggctgac
     3721 cgatgtggtc gggcgcctga tcgccgaagg catgcgcaca gcgcttggcc agaccatcat
     3781 catcgagaat gtcggcgggg ccaccggcag catcgggact gggcgagtgg cgcgcgccgc
     3841 gcccgacgga tacacgatca ccgtcggcat ctggaacacg catgtcgcca acgggataac
     3901 ctacgcgctg cagtacgatg tggtcagcga tttcgaaccg atcgcgctgc tcgcggacgc
     3961 gccgctactg ttcctgggaa agaaagatct gccagcgaac agccttaagg agctgattgg
     4021 ctggctcaag gagaatcccg acaaggcatc gatgggctcg acgggggtgg gcggtccggg
     4081 ccatctgctc gcgctgctgc tgcaagaaaa aaccggcacg cgctttaacc tggtgcctta
     4141 tcgcggcgtc aacccatcgg tgcaggatct gattgcgggg cagatcgaca tgacgatctc
     4201 gaacaccgcc accgcgatgc cgcacgtacg cgcgggcagg gtcaaggcg
//
LOCUS       DATLJT010000739         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1655054,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000739 DATLJT010000000
VERSION     DATLJT010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655054"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1023)
                     /locus_tag="VM910_17010"
     CDS             complement(205..1023)
                     /locus_tag="VM910_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI64262.1"
                     /translation="MQYVEANGAKIPAIGLGTWELRGRACARIVEQALRLGYRHIDTA
                     QIYENEREVGEGVRASGVKRGEVFVTTKVWTNHFAPHDLERSAKESLVKLRMSEVDLL
                     LLHWPNPQVPLAETLGALAHAKQMGLARHIGASNFTVALLTEAVATCAEPLVCDQVEY
                     HPYIDQTKVRQACAQHHIGLVAYSPIAKGAIKKDQTLKRIGWAHRKSPAQVCLRWLVQ
                     QNVSAIPRTSRIERLSENLDIFDFTLSDDEMADIFALASASGRLTDFSFAPKWD"
     gene            complement(1105..1404)
                     /locus_tag="VM910_17015"
     CDS             complement(1105..1404)
                     /locus_tag="VM910_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI64263.1"
                     /translation="MQRLRLKADGRIVELRDGQEFPLAPDAFTAPVESGPPEVRALRR
                     RARLTQQEFAARLGVPVETIRNWEQGKRIPRGPARALLAVIAHAPETVFAALAKT"
     gene            complement(1486..>2014)
                     /locus_tag="VM910_17020"
     CDS             complement(1486..>2014)
                     /locus_tag="VM910_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HVI64264.1"
                     /translation="NFWTIAKGRFNWLLVNLATAFLASFVLGLFEGQLERMVALAVLA
                     PIVASQGGNAATQTMTVAVRALATRQLGSGNALRVIFRESLVGLVNGVAFAVITGVAA
                     VIWFKTPGLGVVIGLAIICNLVAGALGGILIPMVLERVRADPAVASGTFVTTVTDVVG
                     FFSFLGIATLWFGLK"
BASE COUNT          378 a          652 c          632 g          352 t
ORIGIN      
        1 gtcacgatgt cgaccgcgat cggctcggcc gtcaccacgc cgaacggatg cacctcgctg
       61 aacacgacca ccagcgccag caccgacaga tgcgcgatcg ccgatgccgt gatgtccggt
      121 cgtatgaact gccgaacgtc cattggattc gatgttcagt gccgtcccct gcccggctct
      181 agcatagcgg ctgaacccgg cgcctcaatc ccattttggc gcaaaactga agtcggtcag
      241 ccggccactc gcgcttgcca acgcaaaaat gtcggccatt tcgtcgtcgg acagggtgaa
      301 atcgaagatg tcgaggttct ccgacagccg ctcgatgcgc gaggtgcgcg ggattgccga
      361 cacattctgc tgcaccaacc agcgcagaca aacttgagcc gggctcttgc ggtgggccca
      421 tccgatccgc ttcagcgtct gatctttctt gatcgcgccc ttggcgatcg ggctgtaggc
      481 gacgagcccg atgtggtgtt gtgcgcaggc ctgtctcact ttcgtctggt cgatataggg
      541 atggtactcg acctgatcgc acacgagcgg ctccgcacag gtggcgactg cttccgtgag
      601 cagcgccacc gtgaaatttg aagcgccgat atgccgggcg aggcccatct gcttggcatg
      661 cgcgagcgcg cccagcgtct cggcgagcgg cacctggggg ttgggccagt gcagcaacaa
      721 cagatcgacc tcggacatgc gcagcttcac caggctttcc ttggcggagc gttcgagatc
      781 gtggggcgca aaatgattgg tccagacctt ggtggtcacg aagacctcgc cgcgtttgac
      841 gccggaggcg cgcacgccct cgccgacctc gcgctcgttc tcatagattt gcgcggtatc
      901 gatgtgacga tagccgagcc gcaatgcctg ctcgacgatg cgggcgcagg cgcggccgcg
      961 cagctcccag gtgccgagtc cgatcgccgg gatttttgcg ccgttggcct cgacatattg
     1021 catgcacggg cactccacct gctcatcgca ttatgtcgcg cgcagcccgt gctgccaatc
     1081 gggacgaagg cgcgagcgcg gacttcaggt tttggcgagg gccgcgaaca cggtttcggg
     1141 cgcgtgcgcg atgacggcaa gcaacgcccg tgcgggtccc cgcggtatac gtttgccctg
     1201 ctcccaattg cgaatggtct cgaccggcac gccaagcctt gccgcaaatt cctgttgcgt
     1261 cagacgcgcg cggcgacgga gagcgcgcac ttcggggggg ccgctttcca caggtgccgt
     1321 aaaggcgtca ggcgcgagcg ggaactcctg cccgtcccgt aattcgacga tccgtccgtc
     1381 cgctttcaac cgcaagcgct gcatgatcct gaccccgacc gacgggtatc atcggcgaag
     1441 cgcgttaacg ttcgattaac gatacaagtg attgctgctg ctggcctatt tcaggccgaa
     1501 ccagagggtc gcaatcccga ggaaggaaaa gaagccgacc acgtccgtca ccgtcgtgac
     1561 aaaggtgccg gaggccacag ccggatcggc cctgacccgc tccagcacca tcgggatcag
     1621 aatgccgccg agtgcgcccg cgacgagatt gcagatgatc gcaagcccga tcacgacacc
     1681 gaggcccgga gtcttgaacc atatcaccgc ggcgacgccg gtgatgacgg caaaggcaac
     1741 gccattgacc agcccgacca ggctttcacg aaagatgacg cgaagcgcgt tgccggagcc
     1801 gagttggcgc gtcgccagcg cccgcactgc gaccgtcatg gtctgggtcg cggcgttgcc
     1861 gccctggctc gcgacgatcg gggccagcac cgccagcgcc accatcctct cgagctgccc
     1921 ctcgaacagg ccgagcacga atgaagccag aaaggcggtc gcgagattga ccaggagcca
     1981 gttgaagcgg cccttggcga tggtccagaa attg
//
LOCUS       DATLJT010000740         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1424562,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000740 DATLJT010000000
VERSION     DATLJT010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424562"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..>2949
                     /locus_tag="VM910_17025"
     CDS             50..>2949
                     /locus_tag="VM910_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI64265.1"
                     /translation="MANLSLKPERGSCVGRALLEARIVQIPDIRKDPEYTLDARRAGV
                     PKGYRTILGVPLLREGTPIGVIALGRHTVRPFTEKQIELVTTFADQAVIAIESARLLN
                     ELRQRTTDLTEALEYHTATSDVLKVVSQSSAELGPVLDTLVATAARICLAESGFIFRL
                     QDGLCRMIASFGIPAEYKDFQARNPIAPGRGTLVGRTVLERRAVHIEDAAADPEYTRI
                     EAVQLGNQRTMLGVPLVRDDALIGVITLARSRVEAFSEKEIALVEIFAHQAVIAIENA
                     RLLSELRQRTTDLTQALDQQTATSEVLQGISSSPGELESVFQAILAHATRLCEASYGA
                     MWLKEGDRFRNAAFHGALPAAYIEQWRRATVGRTAPLGRVAQSRKPLQIADLRQDQTY
                     LEGHPLTITAVDVAGIHTLAIVPMLKEDEFVGGISIYRKEVRPLTDKQIELVQNFANQ
                     AVIAIENTRLLNELRRSLQQQTATADALKVISRSTFDLKTVLQTLVESTARLCEADQA
                     TITRQIGGKFFRAEAYGFSSEFMDYVRDVPVEPERGTVHGRALLEGQIIHIPDALADP
                     DYTWAEAQRLGGFRTILGVPMLREGVPIGVLALTRSEVRPFTDKQVELVTTFADQAAI
                     AIENVRLFEEEATARAAAEAARDAAERARTEAAASRDAAEQARCEAEAANLAKSTFLA
                     TMSHEIRTPMNGVLGMIEVLERQGVAEDQRRIIATMRESAQALLRIIDDVLDFSKIEA
                     GRLELEAIPFSLSGLVEGVLDTFRPQVLAKGLTLDAEIDAGSQDALVGDPTRVRQILF
                     NLLSNAIKFTDHGVVRVHAGTLPLGGGSTRTTLAVSDTGIGMGAEQLARLFQPFVQAD
                     SSTTRQFGGTGLGLSIIQRLAQVMGGDVAVESAPGAGSTFTVKLTLHAAPADSPLKTL
                     LRPVARPSARVAARSGAGPRVLVVEDHPVNRDVLMLQLKLLGIGADTAENGV"
BASE COUNT          577 a          905 c          934 g          533 t
ORIGIN      
        1 gtatcgcctg atcgcgaggt tcgggacctc gcgcgcgcac gatgactata tggccaatct
       61 gtccctcaag cccgagcgag ggtcgtgtgt cgggcgggcc ttgcttgaag caagaatcgt
      121 ccagatcccg gacatcagga aggatcccga atacacgctc gacgcacgga gagcgggagt
      181 acccaagggg tatcgcacca ttcttggcgt tccgcttctg cgggaaggaa cgcccatcgg
      241 ggttatcgcg ctggggcgcc acaccgtgcg gccgttcacg gagaagcaga tcgagctggt
      301 cacgacgttt gccgatcagg cggttatcgc catcgagagt gcgcggttgc tcaacgaatt
      361 gcgccagcgc accaccgacc tcaccgaggc actcgaatac catactgcga cgagcgacgt
      421 gctgaaggtc gtcagccagt cgagtgccga gctcgggccg gtgctcgaca cgctcgtcgc
      481 gaccgcggcg cgcatctgct tggccgaatc cggttttata ttccggctgc aggacggtct
      541 ctgccgaatg attgcctcgt tcgggatacc ggcagaatat aaggacttcc aagcgcgcaa
      601 tccgattgcg ccgggccgcg gcacgttggt cggtcggacg gttttggagc ggcgcgcggt
      661 gcatattgaa gatgcggcgg ccgatcccga atacactcgg atcgaagcgg tgcagctcgg
      721 taatcagcgg acaatgctcg gggtgccgct ggtccgcgac gatgcactga tcggtgtcat
      781 cacccttgcc cggtcgcgtg tcgaagcttt ctctgaaaaa gagatcgcgc tggtcgaaat
      841 cttcgcccac caggcggtga tcgcgatcga gaatgctcgg ctcctgagtg aattgcggca
      901 gcgcacaaca gacctcaccc aggcgctgga tcagcagacg gcgacctcgg aggtgttaca
      961 gggcatctcc agttcgccgg gtgagctgga atcggtgttc caggccatac tggcacacgc
     1021 gacgcggctc tgcgaagcca gctacggcgc catgtggctc aaagaaggcg atcgctttcg
     1081 caacgctgca ttccacggtg cgctgccggc ggcatatatc gagcagtggc ggagggccac
     1141 ggtgggccgc acagcccctc tgggccgcgt tgctcaatcc cgaaagccgc ttcagatcgc
     1201 tgacctgcga caagatcaga cgtatctcga aggccatcca ttgactatta ccgcagtcga
     1261 cgtcgccggc atccacacat tggcaatcgt gcccatgctc aaggaggatg agttcgtcgg
     1321 cgggatctcc atttatcgta aggaggttcg gccgctcacc gacaagcaga ttgagctggt
     1381 ccagaacttc gccaaccaag ctgtgatcgc catcgagaat acccgtctgc tcaacgagct
     1441 gcgccgatca ctgcagcagc agacggccac agccgatgcg ctcaaagtca tcagccgctc
     1501 gaccttcgac ctcaagactg tacttcagac gctggtcgaa tcgaccgcac gactgtgtga
     1561 ggccgatcaa gccaccataa cgcggcagat cgggggcaag ttctttcgcg cagaagctta
     1621 tggtttttct tccgagttca tggattacgt cagagacgta cccgttgagc cagaacgtgg
     1681 gacggttcac gggcgagcat tgctcgaggg ccagattatt cacatccccg atgcgctagc
     1741 tgacccggac tacacctggg cggaagcgca gaggctgggt ggctttcgca ccatcctcgg
     1801 ggttccgatg ttgcgcgagg gcgtcccaat cggcgttctg gcattgacgc gctccgaggt
     1861 gcggccattt accgacaaac aggtcgagct ggtgaccacg ttcgccgacc aggcagcaat
     1921 cgcaatcgag aacgtgcggc tgttcgagga ggaggccacg gccagggcag ccgccgaggc
     1981 cgcgcgcgac gcggctgaac gggcgcgcac cgaggccgcg gcctcgcgcg acgcagccga
     2041 gcaggcgcgt tgcgaggccg aggccgccaa cttggcgaag tcgacgtttc tcgccaccat
     2101 gagtcacgaa atcagaacgc ctatgaacgg cgtgcttggc atgatcgaag tgctggagcg
     2161 gcaaggggtc gccgaggatc agcgacgcat catcgcgacc atgcgagaat cggcgcaggc
     2221 cctgctgcgc atcattgacg acgtgctcga cttctcgaag atcgaggccg gacggctgga
     2281 gcttgaggct ataccgttct cgctaagcgg cctggtcgaa ggggtgctag acacatttcg
     2341 gccgcaggtg ctcgccaaag gtcttacgct cgatgccgaa atcgatgccg gctcgcagga
     2401 cgcattggtt ggggatccga cccgggtacg gcaaattctg ttcaatctgt taagcaacgc
     2461 catcaaattc acagaccacg gcgttgtgcg ggtgcacgcc ggcaccttac cgctcggtgg
     2521 cggcagtacg cgcacaacgc tcgcggtgtc cgacaccggc attgggatgg gcgcagagca
     2581 actcgcccgg ctcttccaac ccttcgtaca ggccgatagc tcgaccacac ggcaattcgg
     2641 aggaaccggt cttggccttt ccattatcca acggctggcg caagtcatgg gcggcgatgt
     2701 cgccgtcgag agcgcacccg gcgccgggtc taccttcacc gtgaagttga cacttcatgc
     2761 ggcgccggca gactcgccgc tcaagacgct actccggcct gttgccagac cttcggcgag
     2821 agtcgccgcg cgttcaggcg cgggcccgcg cgtcctggtg gtcgaggatc atcccgtgaa
     2881 ccgcgacgtg cttatgctcc agctcaagct gctggggatc ggcgccgaca ctgcagagaa
     2941 tggcgtcga
//
LOCUS       DATLJT010000741         1734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3951940,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000741 DATLJT010000000
VERSION     DATLJT010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951940"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VM910_17030"
     CDS             complement(<1..852)
                     /locus_tag="VM910_17030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI64266.1"
                     /translation="MKPLQGVRIVSVEQFGAAPYGTMLLADLGAEVIKVENAAIGGDP
                     ARKTGPYFLGTNDSEYYQAFNINKKSVAIDLRSAEGRAALTKLVATADALVNNLRGDL
                     PAKMGLDYKTLSGVNPKLVCVHVSAYGRDNDRASWPGYDYLMQAESGLMHLTGEPDGP
                     PARLGAPSIIDQTTGLTAAVGLLSAVIQARTTGKGCDVDTCLLDVALHQLGYVATWYL
                     NEGHASTRQPRSAHYSVAPVQTFPTSDGWLFIMCMTDKFWGELLAAIDRTDLASDPRF
                     ATQALRQE"
     gene            complement(849..>1734)
                     /locus_tag="VM910_17035"
     CDS             complement(849..>1734)
                     /locus_tag="VM910_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI64267.1"
                     /translation="MLALAIEKFGTQQQKSRWLPKLATGEIRGGLALTEPDAGTDLQG
                     IRMTARRDGDHYVINGTKTWITNGAEGSCLALLVKTNPQAQPRYNGMSLFIAPKQEGF
                     SVGRRLPKLGYKAIDTAELVFDNYRLPIDHLIGGVEGRGFFQVAGGLELGRINVASRG
                     VGIAEGALKLAAEYAQIRKTFGKPICEHQAIQLKLGEMATRARAARLLTLDAAEAFDR
                     GERCDMEAGMAKYFASEAALENSIEGMRIHGAYGYSKEFDIERLYRDAPLTCIGEGTN
                     EMQRIIISKQFIKRNPAV"
BASE COUNT          293 a          545 c          548 g          348 t
ORIGIN      
        1 ttcctggcgc aaggcctgcg tcgcaaagcg cggatcggag gcaagatcgg tccggtcgat
       61 cgctgcgagc agctcgcccc agaacttgtc ggtcatgcac atgatgaaca gccagccgtc
      121 cgaggtcggg aacgtctgca cgggcgccac cgaatagtgc gcgctgcgcg gttggcgcgt
      181 cgaggcgtga ccttcgttga gataccaggt tgcgacgtag ccaagctgat ggagtgcgac
      241 gtcgagcagg caggtatcga cgtcgcagcc tttgccggtt gtcctcgcct ggatcaccgc
      301 agagagtaac ccgactgcgg cggtcaatcc tgtggtctgg tcgatgatcg aaggcgcccc
      361 gagccgggcg ggaggcccat cgggctcgcc ggtcagatgc atcaggccgg actcggcctg
      421 catcaggtaa tcgtatcccg gccatgacgc gcggtcgttg tcgcgaccgt aagccgagac
      481 atgcacgcag accagcttcg gattgacgcc cgatagtgtc ttgtaatcga ggcccatctt
      541 cgccggcaaa tcgccgcgaa gattgttcac caacgcgtcc gctgtggcga cgagctttgt
      601 caatgccgcc ctgccctcgg ccgagcgcag gtcgatcgcc acgcttttct tgttgatatt
      661 gaatgcctgg taatattcgc tgtcattggt ccccaggaaa taggggccgg tcttgcgcgc
      721 ggggtcgccc ccgatcgcgg cgttctcgac cttgatgacc tcggcgccga gatccgcgag
      781 cagcatcgtg ccgtaaggtg ccgcaccaaa ctgctcgact gaaacgatgc gcacgccctg
      841 aagtggcttc atacggcggg attcctcttg atgaattgct tcgagatgat gatgcgctgc
      901 atctcgttgg tgccctcgcc gatacaggtg agcggcgcgt cgcggtacag gcgctcgatg
      961 tcgaattcct tggaatagcc gtaggcgccg tggatgcgca tgccctcgat cgagttttcg
     1021 agcgcggctt cgctggcaaa gtatttggcc atgccggctt ccatgtcgca gcgttcaccc
     1081 cggtcgaagg cctccgctgc gtcgagcgtc agcaggcgcg ccgcccgcgc gcgcgtcgcc
     1141 atctcgccta gtttgagctg gatggcctgg tgctcgcaga tcggcttgcc gaacgtcttg
     1201 cgaatctgag cgtattcggc agcgagtttc agcgcgcctt ccgcgatgcc gacgccgcgc
     1261 gacgcgacgt taatgcggcc gagctccagg cccccagcca cctggaaaaa gccgcggcct
     1321 tcgacaccgc cgatcaaatg atcaatcggc aggcggtagt tgtcgaatac gagttccgcg
     1381 gtgtcgatcg ccttgtagcc gagcttcggt agcctgcgac caaccgaaaa gccctcctgc
     1441 ttcggcgcga tgaacaggct catcccgtta tagcgcggct gcgcctgcgg gttggtcttg
     1501 accaacagcg ccaggcatga gccctcggcc ccgttggtga tccaggtctt ggtgccattg
     1561 atgacataat gatcgccatc gcgtctggca gtcatgcgga tgccctgcag gtcggtgccg
     1621 gcgtccggct ccgtcagggc tagtccgccg cgaatctcgc cagtcgcaag cttcggcagc
     1681 catcgcgact tctgttgctg tgtgccgaac ttctcgatcg cgagcgccag catc
//
LOCUS       DATLJT010000742         1850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3952092,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000742 DATLJT010000000
VERSION     DATLJT010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3952092"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VM910_17040"
     CDS             complement(<1..1652)
                     /locus_tag="VM910_17040"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64268.1"
                     /translation="MSNRAIAHKLGVTEKAIRKQVGPTRGAASGQLALPEIRPPKKSA
                     ATAPPASSAGGDDDDDDDPGGKRSPSAAPPPANDDEPVPKSLDRDASNRTFDRQLAYL
                     GLLKDAAPLFRDGSSIPGAGVLLALPCLIESGLLRISRKLYGEIGPAFYGLRTTLLTL
                     LLMALLRIKRPEHLKERDPAAFGRLLGLDRAPEVKTLRRRLTCLAARHCAEQLGAELA
                     RVRVGQRGHLMGFLYVDGHVRAYHGQRSISSNAYVARRHLAMPASTDYWINDSSGDPL
                     LVITGEIDAALTKAMPGLLREVREVVGERKVTIVFDRGGWSPKLFATMIKDGFDVLTY
                     RKGRCRHINERRFVRRRAVLDGRSVDYLLHDEPVRLLNGKLRLRQVTRLCDGGHQTTV
                     ITSRWDLRDIEVAYRMFERWRQENFFKYMREEFLLDALVDYQIEPEDPTRTIPNPERR
                     ALDKEIRAARADVARLECELGAAAANNAEQRRPTMRGFKIANARLGKQLRKALTHLTR
                     LLKKRRDIPKRVEVRELSEQAMVKLATERKHLTDIIKMVAYQA"
BASE COUNT          331 a          596 c          572 g          351 t
ORIGIN      
        1 gcctgatagg caaccatctt gatgatgtcg gtcaggtgct tgcgttccgt ggcgagcttg
       61 accatagcct gctcgctgag ttctcgaact tcgacgcgct tgggaatgtc gcggcgtttc
      121 ttgagcagcc gcgttaggtg ggtcagtgcc ttgcgcagtt gcttgccaag cctggcattg
      181 gcaatcttga agccgcgcat ggttggccgc cgctgttcgg cattgttggc ggcggcggcc
      241 cctagttcgc attcgagcct agcgacgtca gcgcgggccg cgcggatctc cttgtccaag
      301 gcgcggcgtt ctggattggg gatggtgcgg gtggggtcct ccggttcgat ctggtagtcg
      361 acgagcgcat cgagcaggaa ctcttctcgc atgtacttga agaagttctc ctgcctccag
      421 cgctcgaaca tgcgataggc gacctcgatg tcacgcagat cccatcggct cgtgatcacc
      481 gtcgtctgat ggccgccgtc gcacaagcgc gtgacttgcc gcaagcgcag cttgccgttg
      541 agcaagcgca cgggctcatc gtgcagaaga tagtcaaccg agcgtccgtc gagcacggcg
      601 cgacggcgga caaagcgccg ctcattgatg tgacggcacc ggcccttgcg ataggtcagc
      661 acgtcgaagc cgtccttgat catcgtggcg aacaatttcg ggctccagcc gccgcggtcg
      721 aagacaatgg tgacctttcg ctcgccaacc acctcccgca cctcgcgaag cagaccgggc
      781 atagctttcg tcaaagcagc atcgatctct ccagtaatca ccagcaatgg atcgccggag
      841 ctgtcgttga tccagtagtc ggtgctggca ggcatggcca gatgacgccg cgccacataa
      901 gcatttgatg aaatagagcg ttggccatga taggcgcgca cgtgaccgtc gacgtacaaa
      961 aagcccatga gatgcccgcg ctgaccgaca cgcacacgcg cgagctctgc gccgagctgc
     1021 tcagcgcaat ggcgcgcggc gagacacgtg agccggcgcc gcagcgtttt cacttccggc
     1081 gcgcggtcga gcccgagcag cctgccgaat gcagctggat cccgctcctt gagatgttcg
     1141 ggccgcttaa tccggaggag cgccataagc aggagcgtca gcaacgtcgt gcgcagacca
     1201 tagaaagccg ggccgatctc accgtagagc ttgcggctga tccgcaacag cccgctctcg
     1261 atcaagcacg gcagagccaa cagcacgccg gctcccggaa tcgaggatcc atcacgaaac
     1321 agcggtgcgg catccttgag cagcccgaga taggcgagct gccgatcgaa ggttcggttg
     1381 ctcgcgtcgc gatcgaggct cttgggcacc ggctcatcat cgtttgcggg cgggggcgcg
     1441 gcgctcggac tgcgctttcc ccctgggtcg tcatcatcgt catcatcgcc cccggccgac
     1501 gatgccggtg gcgcagtcgc cgccgacttt ttcggtggcc ggatctcagg cagcgcaagc
     1561 tgcccacttg ccgcgcccct ggtaggtccg acctgtttac gaatggcttt ctcagtgacg
     1621 ccgagcttat gcgcgatcgc ccggttgctc atcccctggc tcttcatctg ctctatgcgg
     1681 cgcagacgtt tcgccgagat gcgtcggcga ccgcgacgcc agcccgcttc gtgaccgagc
     1741 gccgccatcc ccccttgggc atagcgccat tggttgcgcc gcaccgtacg caccgaacag
     1801 ccaaacgcac gcgcaacgtc cgtttgcagg gcaaagcctg actcaacaag
//
LOCUS       DATLJT010000743         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_506410,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000743 DATLJT010000000
VERSION     DATLJT010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506410"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VM910_17045"
     CDS             <1..374
                     /locus_tag="VM910_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVI64269.1"
                     /translation="KDAEANAAADKKRREAVDAKNHADALVHSTEKALAEHGSKIAET
                     ERRAIEDAVSDLKEALKGDDAEAIKAKTNTLAQASMKLGEAMYKQQAEADAAKAGAGG
                     KDDVVDAEFTEVDDDKNKKSA"
     gene            515..1648
                     /gene="dnaJ"
                     /locus_tag="VM910_17050"
     CDS             515..1648
                     /gene="dnaJ"
                     /locus_tag="VM910_17050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVI64270.1"
                     /translation="MSIKRCYYETLEVDRDADENKLKAAFRKLAMKWHPDRNPGDKAS
                     EVRFKEINEAYEILKDSDKRAAYDRFGHAAFEQGGMGGAGPGFGAGFASSFSDIFEDL
                     FGMAGQRARGGRERGADLRYNMEITLEEAFQGKTAQIEIPVSVTCESCSGTGAKTGTK
                     PKTCSMCGGAGRVRQAQGFFTLERTCPGCQGRGQMIEDACPSCSGSGRVTRERTLSVN
                     IPQGVEDGTRIRLAGEGEAGFRGGPSGDLYIFLSLGAHPFFQRDGADLHCRVPISMVT
                     AALGGEFEVPTIDKGKTKVKIPAGTQSGRRFRIASKGMPVLRSRQSGDMYVQVVVETP
                     QNLTKKQQELLAEFEKLSSGATQPEAAGFFTKVKDFFGNRATN"
     gene            1853..>2353
                     /locus_tag="VM910_17055"
     CDS             1853..>2353
                     /locus_tag="VM910_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HVI64271.1"
                     /translation="MPLQSSVRASKKPLRLDDEVRFLRSWIEKPLHVGAVMPSGRLLA
                     RTMAQYVEVKSEGPVVELGPGTGAITSALIDHGVDQKRLVLVEYNPGFCALLRDRYPQ
                     AKVVQGDAYSLRDTLWNVLSVPAAAMVSGLPLVTKPMLTRLKLIRDAFVALAPGAPFV
                     QFTYSVA"
BASE COUNT          460 a          727 c          760 g          406 t
ORIGIN      
        1 tcaaggacgc cgaagccaat gccgcggccg acaagaagcg ccgcgaggcg gtcgacgcca
       61 agaaccatgc ggacgcgctg gtgcactcga ccgagaaggc gctggccgag cacggttcga
      121 agatcgcgga aaccgagcgc cgcgccatcg aggatgcggt cagcgacctg aaggaggcgc
      181 tgaagggcga cgatgccgag gcgatcaagg ccaagaccaa cacgctggcg caggcttcga
      241 tgaaactcgg cgaggccatg tacaagcagc aggccgaagc cgacgccgcc aaggccggcg
      301 ccggtggcaa ggatgacgtg gtcgacgcgg agttcaccga ggtcgacgac gacaagaaca
      361 agaagtctgc ttgagcgacg gacaaggatc atgaccctcg ttccaagagc gcgtgttgcg
      421 tctcgaagga atgagggtca tttttcgtta agcctaaggc tgcatttttc ggagtgaacc
      481 tcggtacgag gaagaagttc gggcgaaatg accgatgtcc atcaagcgct gctattacga
      541 aacccttgaa gtcgaccgcg acgcggacga aaacaagctc aaggcggcct ttcgcaagct
      601 cgcgatgaaa tggcatcccg accgcaatcc tggcgacaag gccagcgaag tacggttcaa
      661 ggaaatcaac gaagcctatg agatcctgaa ggacagcgac aagcgcgctg cctacgaccg
      721 cttcggccat gcggctttcg agcagggcgg catgggcgga gccggccccg gcttcggtgc
      781 ggggtttgct tcatccttct ccgatatttt cgaagacctg ttcggcatgg ccgggcagcg
      841 cgcccgcggc ggccgcgaac gcggcgcgga cctgcgctac aacatggaga tcacgctcga
      901 ggaagcgttc cagggcaaga ccgcgcagat cgagatcccg gtttccgtca cctgcgaatc
      961 ctgctcgggc accggcgcca agaccgggac caagcccaag acctgttcga tgtgcggcgg
     1021 cgccggacgc gtgcggcagg cgcagggctt cttcacgctc gagcggacct gcccgggctg
     1081 tcagggccgc ggccagatga tcgaggacgc ctgcccgtcc tgttcagggt cggggcgggt
     1141 gacgcgcgag cggacgctct cggtcaacat cccgcagggc gtcgaagacg gcacgcgaat
     1201 caggctcgcc ggcgagggcg aagccggctt ccgcggcggc ccgtccggcg atctctacat
     1261 tttcctctcg cttggcgcgc atccgttctt ccagcgcgac ggcgctgatc tgcattgccg
     1321 cgtgccgatc tcgatggtga ccgcagcgct tggcggcgaa ttcgaggtgc cgaccatcga
     1381 caagggcaag accaaggtca aaatccctgc aggcacgcag tccggccggc gtttccgtat
     1441 cgcgtccaag ggcatgcccg tgctgcgctc gcgccagagc ggcgacatgt atgtccaggt
     1501 cgtggtcgag acgccgcaga acctgacgaa gaagcagcag gaattgctgg ccgagttcga
     1561 aaagctctcc tccggcgcaa cccagccgga agccgcgggc ttctttacca aggtcaagga
     1621 cttcttcggc aatcgcgcga ccaactaggc gcgccgccgg cgcggggcca ttatgattcg
     1681 catcgccgtg cggaaaaatt cagtggacgc atggtcttat ctgcgaatcg ccggcatcat
     1741 ccaagctcac atgggacacg gttcgtcttg accgctcgtt ctgctcccta tacgtcttta
     1801 tgactgtttt ctgacatcca ccgcaaaatg cgccgtcccg tctggtagcg aaatgccact
     1861 gcaatcgtcc gtgcgtgcgt cgaaaaagcc ccttcgtctc gacgatgagg tccgcttcct
     1921 ccggtcatgg atcgaaaagc cgctgcatgt gggcgcagtc atgccatcgg ggcggctgct
     1981 tgcccgcacc atggcgcaat atgtcgaggt caaatccgaa ggacctgtag ttgagctcgg
     2041 ccccggcact ggcgcgatca ccagcgcatt gatcgatcat ggggtcgatc agaagcgcct
     2101 ggtgctggtc gagtacaatc ccggcttctg cgctctgttg cgcgaccgct atccgcaagc
     2161 gaaggtggtg cagggtgatg cctattcgct gcgcgatacg ctctggaacg ttctgagcgt
     2221 gcctgctgcg gcgatggtgt cggggctgcc gctggtgacc aagccgatgc tgacgcgcct
     2281 gaagctgatc cgcgatgcct tcgtcgcgct cgcgcccggc gcgcccttcg ttcagttcac
     2341 ctattcggtg gcg
//
LOCUS       DATLJT010000744         4599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3262989,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000744 DATLJT010000000
VERSION     DATLJT010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3262989"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VM910_17060"
     CDS             <1..319
                     /locus_tag="VM910_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HVI64272.1"
                     /translation="VDDAWVEGQSLIATVEDVELIDPDLSGERLLYRLFHERGVRVFR
                     PLPLAASCSCSRDAVFAMLKSFAPKERADMVKDGKVVVTCEFCSSVYEFTPHEAGVEE
                     GAG"
     gene            complement(325..1068)
                     /locus_tag="VM910_17065"
     CDS             complement(325..1068)
                     /locus_tag="VM910_17065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HVI64273.1"
                     /translation="MQDRLARQTILSAALALSALQFLFSGSANSLVGADLVDLSLQRY
                     TVIVANAKGHCTGVVLAQNIVLTAAHCVTNMQNLWIGGNRGFGDPSGPPVELGRIAEV
                     AVHPRYDSTTIHSIDLALLRVATPLPDRFLPAFFGAPVPSDGDDLVVAGYGMSADKDP
                     MAGKILRMGLLRLARRYKDVLMLVSTREEASGAGHGDSGGPVFSYHGMLALVAVMVSG
                     GGIAHATYAVPIAPNYAWIKETMAKWGAS"
     gene            complement(1104..1496)
                     /gene="apaG"
                     /locus_tag="VM910_17070"
     CDS             complement(1104..1496)
                     /gene="apaG"
                     /locus_tag="VM910_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HVI64274.1"
                     /translation="MYRAVTRKIEVTVEPNFMPERSSAENSRYFWSYTIVITNTGDET
                     VQLRTRYWIITDASGRQQEVRGEGVVGEQPVLAPGEHFEYTSGVPLTTASGFMTGRYQ
                     MVSESGERFEIEVPTFSLDSPGLKRVLN"
     gene            1851..3110
                     /locus_tag="VM910_17075"
     CDS             1851..3110
                     /locus_tag="VM910_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HVI64275.1"
                     /translation="MSIADRAATSPAAGAADAGASGAASVPAAPAGAIALPLTGEREL
                     RLDLFRGLALWLIFIDHLPPSLITWFTIRNYGFSDATEIFIFISGYTAAFVYGRAMLE
                     HGLVVATARILRRAWQIYVAHVFLFTIFLAEISYVATSFENPLYTEEMGIMDFLKQPD
                     VTIVQALLLRFRPANMDVLPLYIVLMLSLPLVLVLMRWKPDLTLALSVLLYAVTWEYD
                     LYLSSYPNGFWAFNPLAWQLLFVFGAWCALGGARRMQKILSSPVTLAVCFAYLFFSFL
                     VTLTWYVPQLGHLMPRRIEQWMYPIDKTDLDVLRFAHFLALAAITVYYLPRDWPPLKS
                     PWLKPLILCGQHSLEIFCLGVFLAFAGHFILAEVGGGAALHALITLSGILIMCGMAWL
                     ISWYKHVADKSASRKGAGGNADMAGGG"
     gene            3110..3862
                     /locus_tag="VM910_17080"
     CDS             3110..3862
                     /locus_tag="VM910_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVI64276.1"
                     /translation="MRPGIVLTLVVAGIVVSVSNLRAEEAAQSCDVPDFLLATDSSLN
                     KVADAVKNGRPLDVLVVGSRSSTIVSSDSSAYPARLQAALKEKLPQLAVNLSVEIQAK
                     KTAEEAAASLAKLAEAKRPTLVIWQTGTVDAMRSVDPDDFRGAVDEGVVALQNAGADV
                     ILMNPQYSPRTETMISLPPYLDNMRAVAQQHDVPLFDRFAIMHQWNDQGDFDLFSAVH
                     GIDLAMRVHDCLGRALSKFVIDAAHLGPAQQN"
     gene            3875..>4599
                     /locus_tag="VM910_17085"
     CDS             3875..>4599
                     /locus_tag="VM910_17085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVI64277.1"
                     /translation="MILPRCHGVWLFDLNSLGRTCGSRGRVKSAALAALILLTPFSLA
                     QARAQSAQAAPQPATSAQQTTPALSPQTTGNAASGNSSGDSQQKSLTARAIDKAKSAA
                     KSASDIFSRVPCLPPKGEANSIGTLPHVARKLASGEPVVIVAFGSSSTAGTGASAPEF
                     TYPNRLADQLRRQYPTANISVINAGVGGEDAPEMMKRLQTSVIDAHPDLVIWQVGTNA
                     VLRNLDPSDTVNLVEEGVARIQAA"
BASE COUNT          806 a         1451 c         1410 g          932 t
ORIGIN      
        1 ggtcgacgat gcctgggtcg agggacaatc gctgatcgca accgtcgaag acgtcgagct
       61 gatcgatccg gatctgtccg gcgagcggct gctctaccgg ctgtttcacg agcgcggcgt
      121 gcgcgtgttc aggccgctgc cgcttgccgc gagctgctcc tgctcgcgcg acgcggtctt
      181 tgccatgttg aagagctttg cgccgaagga acgcgccgac atggtgaagg acggcaaggt
      241 cgtggtcacc tgcgagttct gcagctcggt ctacgagttc accccgcacg aagcgggcgt
      301 ggaagagggc gcggggtagc gccttcagga ggcgccccac ttcgccatcg tctccttgat
      361 ccacgcataa tttggcgcga tgggaacggc gtaagtggcg tgagctatgc caccgccgct
      421 gaccatgacc gccaccaagg cgagcatgcc gtgatagctg aagacgggac cgccggagtc
      481 gccgtggccg gcccctgaag cctcctcacg cgtactgacg agcataagaa cgtccttgta
      541 tcttcgggcg aggcgcagca gccccatgcg aaggattttg cctgccatgg gatctttgtc
      601 cgcactcatg ccatatcccg ccacgactag gtcgtcgcca tcgctcggga cgggcgcgcc
      661 aaagaatgcg ggaaggaagc gatcgggcaa cggtgttgca actctgagca acgcaaggtc
      721 gatcgaatgt atggtcgtgg aatcgtagcg cggatgcacg gcaacctcgg caatccgacc
      781 gagctccacc ggcggaccgg acgggtcgcc aaaaccgcga ttgccgccga tccagaggtt
      841 ttgcatattc gtcacgcaat gggccgcggt cagcacgatg ttttgcgcca gcacgacgcc
      901 ggtgcagtgg cctttcgcgt tcgcgacgat gaccgtgtag cgctgcaggc tcagatcgac
      961 gagatcggca ccaacgaggc tattggccga cccggaaaac aggaactgca aggccgacag
     1021 cgctaacgcg gccgaaagga tcgtctgtct tgccagccga tcctgcatgt gccccgcctg
     1081 tctccgcgga tcgcgctcgc gtgtcagttc agcacgcgct tcaaacccgg gctgtcgagc
     1141 gaaaaggtcg gcacctcgat ttcgaaccgc tcgccggact cgctgaccat ctggtagcgg
     1201 ccggtcatga agcccgatgc ggtcgtcagc ggcacgccgc tggtatattc gaagtgctcg
     1261 cccggtgcga gcacgggctg ctcgccgacc acgccctcgc cgcgcacttc ctgttgccgg
     1321 cctgacgcgt cggtgatgat ccagtatcgc gtccgaagct gtaccgtctc gtcgccggtg
     1381 ttggtgatga cgatggtgta ggaccagaag taccgcgaat tttccgccga cgaccgctcc
     1441 ggcatgaagt tcggctcgac ggtgacttcg atctttcggg tgacggcgcg gtacatggcg
     1501 ccatcatagc gaaaagtcgt tgccgaacca atcggagtga gctgtgacta acgcgtccgc
     1561 acgtcttaac cttgccttga aaaaggggga ggcgctttgt ccggcgatga gcaaagcggc
     1621 agggtttcga tctgccgaac aagccgttaa ctggtggctt cttcccggtt ctccccgggg
     1681 gagggagtgt cccttgcaac gcatcccatc gcggtttcga cagagttcca gcttaaacgc
     1741 cacgcaaccc tgtcgagcaa aacttcgtaa ctttgcttgc aagtgacgcc ccggtacgat
     1801 caatcctgtc gcgccggccg tttccgccgg cgcagaggtg gtgcctgacg atgtccattg
     1861 ccgatcgcgc tgccacgtct ccggctgccg gcgcagccga tgccggcgca agcggcgcgg
     1921 cctccgtgcc cgccgctccg gccggcgcga tcgcgcttcc cctaaccggc gagcgcgagc
     1981 tcaggctgga cctgtttcgc ggccttgcgc tgtggctgat cttcatcgac cacttgccgc
     2041 cgagcctgat cacctggttc accatccgca attacggctt ttccgacgcc accgagattt
     2101 tcattttcat ctccggctac accgcggcct tcgtctacgg ccgcgccatg ctggagcatg
     2161 gcctcgtggt cgcgaccgcg cgtatcctgc gccgcgcctg gcagatctac gtcgcgcatg
     2221 tgttcctgtt cacgatcttt ctcgcggaga tttcatacgt cgcgacctct ttcgagaacc
     2281 cgctctacac cgaagagatg ggcattatgg attttctcaa gcagcccgac gtcaccatcg
     2341 tgcaggcgct tttgttgcgt ttccgtcccg ccaacatgga cgtgctgccg ctttatatcg
     2401 tgctgatgct gtcgctgccg ctggtcctgg tgctgatgcg ctggaagcct gacctcacgc
     2461 tggcgctgtc ggtcttgctt tacgccgtca cctgggaata cgacctctat ctctcgtctt
     2521 atccgaacgg cttctgggcg ttcaatccgc tcgcatggca attgctgttc gtgttcggcg
     2581 cctggtgtgc gctgggcggc gcgcgtcgca tgcaaaaaat cctgtcgtcg ccggtcacgc
     2641 ttgcggtctg cttcgcctat ctgttcttct cgtttctggt gacgctgacc tggtacgtgc
     2701 cgcagctcgg ccacctgatg ccgcgccgca tcgagcagtg gatgtatccg atcgacaaga
     2761 ccgatcttga cgtgctgcgg tttgcgcatt ttctggcgtt ggcggcgatc accgtctact
     2821 atctgccccg ggattggccg cctctgaaat cgccctggct caagcccctg atcctttgcg
     2881 gacagcattc gctggaaatc ttctgtctcg gcgtgtttct ggcctttgcc gggcatttca
     2941 tcctggccga ggtcggcggc ggtgccgcac tgcacgcact catcactctc tccggaatcc
     3001 tgatcatgtg cggcatggcg tggctgattt cgtggtacaa gcacgtggcc gacaaaagcg
     3061 cttcacgcaa gggcgccggc ggcaacgccg atatggcggg aggaggttga tgcggccggg
     3121 gattgtcctc acccttgtgg tggctgggat tgttgtgagc gtaagcaact tgcgtgccga
     3181 ggaggccgcc caaagttgcg acgtgccgga ttttctgctg gcaaccgaca gttcgctcaa
     3241 taaagtagcc gatgccgtca aaaatggccg cccgctggat gttctcgtgg tcggcagccg
     3301 ctcctcgacc atcgtgagct ccgactccag cgcctacccg gcgcggctgc aagcggcgct
     3361 gaaggagaag ctgccgcaat tagccgttaa tctttccgta gaaatacagg ctaaaaaaac
     3421 cgccgaggag gcggccgcga gcctggccaa gctggcggag gccaagaggc cgactttggt
     3481 gatatggcag accggtacgg tcgatgctat gcgatccgtc gatcctgatg attttcgcgg
     3541 cgcagtcgac gaaggggttg ttgcgctgca aaacgcaggc gccgatgtga tcctgatgaa
     3601 tccgcaatac agtccgcgca ctgagacgat gatttcactg ccaccctatc ttgataatat
     3661 gcgtgccgtg gcgcagcagc acgacgtccc gctgttcgac cgcttcgcca ttatgcatca
     3721 gtggaatgat caaggagatt tcgacctttt cagcgccgtg cacgggattg accttgcgat
     3781 gcgcgttcat gattgcctgg gccgggcgct gtcgaaattc gtcatcgacg ccgcgcatct
     3841 cggtccggcg caacagaatt aggaatctag gttaatgatc ttgccgcgtt gtcatggcgt
     3901 ctggcttttc gatttgaact ccctgggtcg aacgtgcgga tcgcgcggcc gcgtcaagtc
     3961 ggcggcactc gcggcgctga tcctgctcac cccattctcg ctggcccaag cgcgcgccca
     4021 gtctgcacag gctgcgcctc agcccgcaac atccgcgcag cagacgacgc cggcactttc
     4081 cccgcagacg acgggcaacg cagcaagcgg caacagctcc ggcgattccc agcagaagag
     4141 cctgacggca agagcgatcg acaaggcgaa atcggcggcg aaatccgcaa gcgacatttt
     4201 cagccgcgtg ccctgcctgc cgccgaaggg cgaggccaat tccatcggaa cgctgccgca
     4261 tgtcgcgcga aagctcgcat ccggcgagcc ggtggtgatc gtggcgttcg gctcgtcgtc
     4321 gaccgcgggc acaggcgcaa gcgcgccgga gttcacctat cccaatcgtc ttgccgatca
     4381 gttgcgccgg caatatccga ccgccaatat cagcgtcatc aatgccggcg tcggcggcga
     4441 ggacgcgccg gagatgatga agcggctgca gacgtcggtc atcgacgcgc accccgatct
     4501 cgtgatctgg caggtcggca ccaatgccgt gctgcgcaat ctcgatccgt ccgacaccgt
     4561 caatctcgtc gaggaaggcg tcgcgcgcat ccaggccgc
//
LOCUS       DATLJT010000745         1845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2574687,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000745 DATLJT010000000
VERSION     DATLJT010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574687"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1234)
                     /locus_tag="VM910_17090"
     CDS             complement(5..1234)
                     /locus_tag="VM910_17090"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI64278.1"
                     /translation="MSDDDIRRDQLHLHERVKHIVDALPQVRAIFLIDRDGRPLVSSQ
                     LRRIPSDFDARERSFFNVHVAGDVGTYVSDVLTPRLTGLNTPFFVLSRRRPSADGTFN
                     GVVAIAVLPQYFEEFYALIGRSPGSLYALLRADGRFLARYPERPDPGLRPGSALHTVI
                     AEGRERSIRTIPQSQIDAVERRVGHRKLEGFPVYVVAGIDSSAIRAEWIATMASHLVF
                     GLPATLFLFLIIGVALRRTRRLHAEAERREAAEAALRQAQRLEAIGQLTGGVAHDFNN
                     LLMIVSGSVQRLRRDLTSEKHTRLLDMITNATNRGESLTRQLLAFSRRQMLTPAVIDL
                     TEQLPELKDMLSRSLRDDITIEVVVPDQGCAVKVDPSELELALLNLAVNARDAMPNGG
                     ALNITAKPVVLKGKAAE"
BASE COUNT          342 a          577 c          574 g          352 t
ORIGIN      
        1 gtccctactc tgccgccttg cccttgagca ctacgggctt cgccgtgata ttcagcgccc
       61 cgccgttcgg catggcatcg cgggcgttga ccgcgagatt gagcaaggcg agctcgagct
      121 cgctcgggtc gaccttgacc gcgcagcctt ggtcgggaac cacgacctcg atggtgatat
      181 cgtcgcggag cgagcggctg agcatgtcct tgagctcggg aagctgctcg gtcagatcga
      241 tcacggcggg agtaagcatc tgccggcgcg agaacgcgag caattgacgc gtgaggctct
      301 cgccgcgatt ggtcgcgttc gtgatcatgt cgaggagacg cgtgtgtttt tccgaggtca
      361 gatcgcgccg aagccgttgc acgctgccac tcacaatcat cagaagattg ttgaagtcat
      421 gcgcgacgcc gccggtcaat tgtccgatcg cttctagacg ctgagcttgc cgcaacgcag
      481 cctccgcggc ctcacgacgc tcggcttcgg catggagccg ccgggtgcgc cgcagcgcaa
      541 ccccgataat gagaaaaagg aacaacgtcg cgggcagacc gaagacaagg tggctcgcca
      601 tggtcgcgat ccactcggcc cggatcgccg agctatcgat gccggcgacg acgtagaccg
      661 ggaagccctc gagcttgcga tgaccgacgc gccgttcgac cgcgtcgatc tgggactgcg
      721 ggatcgtgcg gatgctgcgc tcccgtccct ccgcaatcac cgtgtgcagc gcgctgcctg
      781 gccgcagacc cgggtcgggc cgctccggat aacgggcgag aaatctgccg tcagcacgga
      841 gcagcgcgta aaggcttcct gggctacggc cgatgagcgc atagaactcc tcgaagtact
      901 gcggcagcac ggcgatggcg accacgccgt tgaacgtgcc gtccgccgac ggccggcgcc
      961 gggagagaac aaagaaaggc gtattgaggc ctgtcaaccg cggcgtgaga acatcgctga
     1021 cataggtacc gacatcgccg gccacgtgca cgttgaagaa gcttcgctcg cgcgcgtcaa
     1081 agtcgctcgg tattcgccgc agctggctgg aaacgagcgg ccggccgtct cgatcgatga
     1141 gaaagatcgc ccgcacctgc ggcagcgcgt cgacgatgtg cttcacgcgc tcgtgaaggt
     1201 gcagctggtc cctgcggatg tcgtcgtcgg acatgcctca tcagctgcag cagtcgaatg
     1261 gctgattgcc gagcggaacg cattgcgcat catttcacaa ttcaatgacg acgacgatga
     1321 gtattttccc cattccaacc gaccgccagg agtctggaaa ggcttgctgc catcggcatt
     1381 ccccgttggt tttggcttaa atttgtccca attgtccgcc gcttgcgcag cgtcaccatg
     1441 ccgacattag gtgttatctc gcccgcaagt atcggaccgc gctcgcgacc gtttcgcgca
     1501 atcgacgtgg ctgttggcgt ccagctttga tggtaacttc ggtttgacca cgactgccgc
     1561 agtccacgac ctccgtcgcc tctacgacac tcgaaattgc ttatgagaat cgatcaagtt
     1621 acggtgatgg tcgcgcctct cgccgcgacc ggacaactca tgttcaaccg acaacaatga
     1681 acatccgcct tcaggcccac tgttatcgca acgggatctg gcgcgccatc gggtgggcgc
     1741 tgttcatcct gcttgctatc gccggagggg cggcggcgca aactgccgtc gatctgcagc
     1801 tcgtgctcgc ggtcgatgcg tcgggcagcg tcgatcaggt tcggt
//
LOCUS       DATLJT010000746         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2574756,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000746 DATLJT010000000
VERSION     DATLJT010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574756"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..826
                     /locus_tag="VM910_17095"
     CDS             35..826
                     /locus_tag="VM910_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64279.1"
                     /translation="MRNNIGKYAATIAWPLLVTALILLTWELSVRALGIRSIILPPPS
                     AVFEAMAQRHDLLLAHLWPSLYLTVLGFLFSVIGGIFVAVLITYSAIVRKGFYPVIVV
                     SQVIPKISIAPLFIVWFGTGTMSSLLLAFLVAFFPMTINAAMGFESIDEDIHRMARTF
                     MGSRWQIFWKIRMPNALPYIFGGMKISITLAIIGVIVSEFVASQEGIGYLIKLAGGLL
                     DTPLMLAAIVALSISGLALYAIIAAAESWAVYWQPPPEAVGVGGG"
     gene            901..1989
                     /locus_tag="VM910_17100"
     CDS             901..1989
                     /locus_tag="VM910_17100"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64280.1"
                     /translation="MTLHRRSLLKLAGATAASAGLGIGAPAIVRAATPVKMTLPWLPL
                     GTFSYAFVANKMGFWDKRGLEVTIDRGFGSGRVCVPVDQGQYDFGILDLAVMMNCAAR
                     GLDLVALAGIWPRSPVGIFSLKEYGITKPQDLEGQTVAFDVGSGDFQLWPAFIKATGI
                     DDKKVNKVTMDAAALIKALVEKQVKAEGNFFGSIAPSLWAQGLEINSMLYEDYGVKMF
                     SNVVACKRSTIEKKPELCEAFIGGLMEGLKYVYLNPEKSVALHLESVKEFQGGSVANQ
                     KVIEYGQAVSTALGVMPAVRQHGLGYMDPDLVATTAKTVETYMGVKNAPPTQTLFTNK
                     FVGAVKLTEAEWTEVEKRSEKYLPRKSS"
     gene            complement(2111..>2855)
                     /locus_tag="VM910_17105"
     CDS             complement(2111..>2855)
                     /locus_tag="VM910_17105"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HVI64281.1"
                     /translation="RKFEIIGGLFGEPYRLARCGVADLEVPAGAEIIIEGEILPNVHE
                     PEGPFSEFTGYASYRSTQNVFVAHRIRMRRDAIFHSVTSGMSRDHILISCITREGEIL
                     NALRRNLPNVRAVHVPHTTCGAFMAFISMKKIAEGEPQMAIMAALGTEQYTKYIIVVD
                     DDVDVFNINDVMWAVATRVRAEKDIVFIPGAKGAILDPTSDPENFTLTKMGIDATRPA
                     GREFAERLTIPDDQRERARSILAAAGVKL"
BASE COUNT          524 a          836 c          924 g          571 t
ORIGIN      
        1 gttcgccatc tgcgcaaggc gatcgaggcc agccatgcgg aataacatcg gcaaatacgc
       61 ggccacgatc gcttggccgc tcctcgtcac ggcgctcatc cttttgacgt gggagctcag
      121 cgtcagggcg ttgggcatcc gcagcatcat cttgccgccg ccatcggcgg tattcgaagc
      181 gatggcgcag cgccatgatc tcctgctcgc gcatttgtgg ccctcgcttt atctgaccgt
      241 gctcggattt ctgttctccg tcatcggcgg cattttcgtc gccgtgttga tcacctactc
      301 cgccattgtc cgaaaaggct tttatccggt catcgtggtc tcgcaggtca tcccgaagat
      361 ctccatcgca cccttgttca tcgtgtggtt cgggaccgga accatgtcga gcctgctgct
      421 ggccttcttg gtcgcgttct ttccgatgac gatcaacgcg gccatgggtt tcgagtcgat
      481 cgacgaggat atccaccgca tggcacgcac cttcatgggc tcgcgctggc agatcttttg
      541 gaagatccgc atgcccaacg cactgccgta tatcttcggc ggcatgaaga tttccatcac
      601 gttggccatc atcggcgtca tcgtgtcgga gttcgtcgcg tcccaggaag gcatcggcta
      661 tttgatcaaa ctcgccggcg gtctgttgga taccccgctg atgctggcgg cgatcgtcgc
      721 gctgagcatc agcgggcttg cgctctatgc gatcatcgcc gccgcggaaa gctgggccgt
      781 ttactggcag ccgccgcccg aggcggtcgg ggttggagga ggatgatgcg ccggaccgga
      841 ctgcatgttt gcgtctcgaa cgaatcggaa gcccgtcgac ctcacagagg agtgacttca
      901 atgacgttac atcgccgttc tttgctgaag ctcgccggcg cgaccgccgc ttccgcgggt
      961 ttgggaattg gggcacccgc gatcgtgcgc gccgcgacgc ccgtcaagat gacgttgccg
     1021 tggctgccgc tcgggacctt cagctacgcg ttcgtcgcga acaagatggg tttctgggac
     1081 aagcgcggtc tcgaagtgac catcgaccgc ggcttcggtt cgggcagagt gtgcgtgccc
     1141 gtggatcagg ggcaatatga tttcggcatc ctcgatctgg cggtgatgat gaactgtgcg
     1201 gcgcgggggc ttgacctcgt ggcgctggcg ggaatctggc cgcggtctcc ggtggggatt
     1261 ttttcgctca aggaatacgg catcaccaag ccacaggatc tcgagggtca gacggtcgcc
     1321 ttcgacgtcg gcagcggcga cttccagctc tggcctgctt tcatcaaggc gacggggatc
     1381 gacgacaaga aagtcaacaa ggtgacgatg gacgcggccg cgctgatcaa ggcgttggtg
     1441 gagaagcagg tcaaggcgga aggaaatttc ttcggcagca tcgcaccgag cctgtgggcg
     1501 cagggcctcg agatcaacag catgctgtat gaggactacg gggtgaagat gttcagcaac
     1561 gtggtcgcgt gcaaacgcag caccatcgag aagaagccgg agctgtgcga ggctttcatc
     1621 ggcggtctga tggaggggct caaatacgtc tacttgaacc cggaaaaatc ggtcgcgctc
     1681 cacctcgaga gcgtcaagga atttcaaggc ggcagcgtcg ccaaccagaa ggtgatcgag
     1741 tacggtcagg cggtcagcac cgcgctgggc gtgatgccgg cggtcaggca gcacgggctg
     1801 ggctacatgg accccgatct cgtcgccacg acggcgaaga cggtcgagac ctatatgggc
     1861 gtcaagaacg ctccacccac gcagacgctg ttcaccaaca agttcgtggg cgcggtgaag
     1921 ctgaccgagg cggaatggac cgaggttgaa aagcggtcgg agaaatacct gccgcgcaaa
     1981 agctcgtaat caggagccac gcgagcggca tcctccgtta gcctctcccg caagaggaga
     2041 gggtatccga gtctgctgcc acgctgtcgt tggtgccggg atccgcctcg tccagcaagg
     2101 cgagtgaggg ctaaagcttg acgccggcgg ccgccaaaat agaccgcgcc cgctcgcgct
     2161 gatcgtcggg gatcgtcaga cgttcggcaa actcgcgtcc agccgggcgg gtggcatcaa
     2221 tgcccatctt ggtcagcgtg aagttctccg gatccgacgt ggggtcgaga atcgcgccct
     2281 tggcccccgg gatgaagacg atgtctttct ccgcccgcac ccgtgtggcc acggcccaca
     2341 tgacgtcgtt gatgttgaag acgtccacgt catcgtcaac gacgatgatg tatttcgtgt
     2401 actgctcggt gccgagcgcg gccatgatgg ccatctgcgg ctcgccttca gcgattttct
     2461 tcatggagat gaacgccatg aaggcgccgc aagtcgtgtg cggcacgtgc accgcgcgca
     2521 cgttcggcag gttgcgacgc agcgcattga ggatctcgcc ttcgcgggtg atgcaggaaa
     2581 tcaagatgtg atcccgcgac atgcccgagg tgacgctatg gaagatggcg tcgcggcgca
     2641 tgcgaatccg gtgcgcgacg aacacattct gcgtgctgcg gtaggaggcg taaccggtga
     2701 actcgctgaa cggaccttcc ggctcgtgca cgttgggcag gatttcgcct tcgatgatga
     2761 tctcggcgcc ggcgggcact tcgaggtcgg cgaccccgca gcgggcgagc cggtagggtt
     2821 cgccgaacag cccgccgatg atttcgaatt ttcgc
//
LOCUS       DATLJT010000747         4220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2343960,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000747 DATLJT010000000
VERSION     DATLJT010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343960"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VM910_17110"
     CDS             complement(<1..119)
                     /locus_tag="VM910_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI64282.1"
                     /translation="MRDNNDMPGHLARRFQQIAVAVFLSEVQEAGFDLTPVQYA"
     gene            237..2165
                     /locus_tag="VM910_17115"
     CDS             237..2165
                     /locus_tag="VM910_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HVI64283.1"
                     /translation="MQFHLNGFQPGDPDVFDPAERVLASGASGSLPEEVDVLIVGCGP
                     AGLTLAAQLSAFPDIKTCIVDQKPGRLLVGQADGVACRTMEMFHAYGFSEQVLKESYW
                     VNETAFWKPDDGKAGNIVRSGKVQDVEDGLSEFPHVILNQARVHDCFLDVMRKSPAKL
                     EPYYSRRLLDLQTDLAASVDDHPVTVRFTRVDPTHEGQPETVKARYVVGCDGARSTVR
                     KSIRRELHGDSANHAWGVMDVLAVTDFPDIRLKTLIQSANDGSILIIPREGGYLVRLY
                     VELTKLDVGERVSNRNITSDDLIAKARRILNPYTLEVKEIAWWSVYEIGQRLTDKFDD
                     VPEEEEGETRLPRVFIAGDACHTHSPKAGQGMNVSMQDAFNLGWKLASVLRKRCAPRL
                     LHTYSAERQAVAKELIDFDREWAAILASAKGEGEGFDPAETQRYFVQHGRYTAGTATH
                     YRPSIITGEPSHQHLATGLVIGKRFHSAPVIRLADAKPVHLGHVARADGRWRIFAFAG
                     ADQPAASNSAIAALCNFLGQGRESPVRRYTPAGDDIDAVIDVCAVFQQDHRELAIETM
                     PAFLLPQKGRYGLYDYEKIFCPDLKGGHDIFAMRGIDRDAGCIVVVRPDQYVAHVLPL
                     DAYAQLAAFFDGFMVRAN"
     gene            complement(2217..2774)
                     /locus_tag="VM910_17120"
     CDS             complement(2217..2774)
                     /locus_tag="VM910_17120"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HVI64284.1"
                     /translation="MTRLVLVLFLLAMTASAVVAYKDPERIARYSDTVSDLLRKRSEM
                     PSRAVQIPRGQSGEFAVQARINGVPAAMVIDTGATSVVLTYETAKAVGLPLELLEYDV
                     DVQTAGGHTKAARLTLDRLAIGRLVERSVPALVVPHGTMKTNLLGMSFLDRLESWEMR
                     ADRLMLRGFPEPVASRSERRNAALN"
     gene            complement(2806..3009)
                     /locus_tag="VM910_17125"
     CDS             complement(2806..3009)
                     /locus_tag="VM910_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HVI64285.1"
                     /translation="MTQDTPCIAVCIIDPRTNLCLGCGRTLPEIARWHRMESAERRAV
                     MVLLPQRMRDAGLSPPATDQTPD"
     gene            complement(3057..>4220)
                     /locus_tag="VM910_17130"
     CDS             complement(3057..>4220)
                     /locus_tag="VM910_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI64286.1"
                     /translation="LEAVSDTVMMFLMLLVIAISSMVAANLPIAALAATVPITVMIAL
                     NFVLSGTFDHYVLAALAVAAEGYFALLAHRLHSTTLATLEARAEKDALIGELEQAKSI
                     SDEARHRAESANVAKSRFLAQMSHELRTPLNAILGFSEVMKSEIFGAHAVPVYKEYSA
                     DIHNSGVHLLNLINEILDLSRIEAGRYELNEEAVALANVVADCHHLMKLRASSRGITI
                     HEVFERSMPKIWSDERAIRQIVLNLLSNSIKFTPQGGEVWLKVGWTASGGQYLSVKDT
                     GSGIAEDEIPVVLASFGQGSNSIKSAEQGAGLGLPIAKSLIDLHGGTFTLKSKLRIGT
                     EVIVTFPPERVMSALAPVTEEAPPLQPESDTSPATDERSRPRHRPIMSAGTGA"
BASE COUNT          789 a         1271 c         1341 g          819 t
ORIGIN      
        1 gcgtattgaa cgggcgtgag gtcgaagcct gcttcctgga cctccgaaag gaacacagcc
       61 accgcaatct gctggaagcg gcgcgcgaga tggcccggca tgtcattgtt gtctctcacc
      121 gaattctcca cgagcaaatg aggtattgac aagtatactg atgttcagca tactgatcaa
      181 tatcgaaaac gcagccaaag ccgatttgaa cggtcaaaga cgggaggaat ggtctcatgc
      241 agtttcatct gaacggtttc cagccgggcg atcctgacgt ctttgaccct gccgagcggg
      301 ttctggcctc gggcgcatcc ggatcgcttc cggaggaggt cgatgtcctc atcgtcggtt
      361 gcggaccggc ggggctgacc ctggcggcgc agttgtctgc atttcccgac atcaagacct
      421 gtatcgtcga ccagaagccc ggccggctcc ttgtcggtca ggctgatggt gtcgcttgcc
      481 gcacgatgga gatgttccac gcctacggct tcagcgagca agtcttgaaa gaatcctatt
      541 gggtcaatga aacggcgttc tggaagccgg acgatgggaa ggccgggaat atcgtgcgca
      601 gcggcaaggt gcaggacgtc gaagacgggt tgtccgagtt cccgcatgtc attctgaacc
      661 aggcgcgcgt gcacgattgc ttcctcgacg tgatgcgcaa atcgccggca aagctcgagc
      721 cgtattattc gcgccgcttg ctggatcttc aaaccgacct tgcggcgagc gttgacgacc
      781 acccggtgac cgtccggttc acgcgggtcg atcccacgca tgagggacag cccgagacgg
      841 tcaaggcccg ctacgtggtc ggctgcgacg gcgcgcgcag caccgtgcgc aagtccattc
      901 gccgcgagct ccacggcgat tccgccaacc acgcctgggg cgtcatggat gtgctggctg
      961 tcaccgattt cccggacatt cgtctcaaga cactgatcca atccgccaac gacggcagta
     1021 tcctcatcat cccgcgggaa gggggctatc ttgtccgcct ctatgtcgag ctcaccaagc
     1081 ttgatgtcgg cgagagggtt tcgaaccgca acatcacgtc ggatgatctg atcgccaagg
     1141 cgcggcggat tctcaatccc tatacgctcg aagtgaaaga gattgcctgg tggtcggttt
     1201 acgagatcgg ccagcggctc accgacaagt tcgacgacgt tccggaggag gaggagggtg
     1261 agacgcgtct gccgcgcgtg ttcatcgccg gcgacgcatg ccatacgcac agcccgaaag
     1321 cggggcaggg catgaacgtc tccatgcagg acgccttcaa tctcggatgg aagcttgctt
     1381 cggtgctgcg caagcggtgc gcgccgcggc tgcttcatac ctactctgcc gaacgccagg
     1441 ccgttgcgaa ggaattgatc gacttcgatc gcgaatgggc cgcgatcctc gcttcggcga
     1501 agggagaagg cgagggattc gatccggccg aaacccagcg gtatttcgtt cagcacgggc
     1561 gttacacggc gggcacggcc acgcactatc gcccatcgat cattaccggc gaaccgagcc
     1621 accagcattt ggcgacagga ttggtgatcg gcaagcgctt ccattccgcg cccgtgatcc
     1681 gtcttgcgga tgccaagccc gttcacctcg gccatgtcgc cagggcggac ggacgctggc
     1741 gaattttcgc ttttgcggga gcagaccagc ccgcagcctc gaattccgcc atcgccgccc
     1801 tgtgcaactt cttgggccag gggcgggagt cgccggtccg acggtacacg ccggcggggg
     1861 acgacatcga cgcagtgatc gatgtctgcg ccgtgttcca gcaagatcat cgcgagctcg
     1921 ccatcgagac gatgcccgct tttcttctcc cgcagaaggg acgctatgga ctttacgatt
     1981 acgaaaagat tttctgtccc gatctcaagg gcggtcacga catctttgcg atgcgtggca
     2041 tcgatcggga tgcaggctgc atagtcgtcg tgcggccgga tcaatatgtt gcccatgtcc
     2101 tgccgctcga tgcctatgcg cagctcgcgg cgttcttcga cggatttatg gtgcgggcga
     2161 attgaacgtc gaatgctgtg cgacgtgatg gcgagtcgac acaaagctat tgcgcattag
     2221 ttgagggcag cgtttcggcg ctcgctgcgt gaggcaacgg gctccgggaa accgcgcagc
     2281 atcagacggt cggcgcgcat ttcccagctc tccagccggt ccaggaagct cataccgagc
     2341 aggttggtct tcatggtccc gtgcggcacc accagcgccg gcaccgaacg ttcgaccagc
     2401 cggccgatcg caaggcgatc gagggtcagc cgcgccgcct tggtgtgacc gccggcggtc
     2461 tgcacgtcga cgtcatattc gagcagttcc agcggcagcc cgacggcctt ggcggtttca
     2521 taggtcaaca ccaccgaggt tgcgccggta tcgatcacca tggcggcagg aacgccgttg
     2581 atcctggcct gcacggcgaa ctcgccgctt tgcccccgcg ggatctgcac ggcgcgtgac
     2641 ggcatctcgg agcgcttgcg caacagatcg gaaacggtgt cgctatagcg cgcaatgcgc
     2701 tcggggtcct tgtaggcgac cacggcgctc gcggtcatgg cgagcaggaa cagcacgagc
     2761 acaaggcgcg tcatgaggcg cctcctgcga aggcgcggga ttggtctaat ctggcgtctg
     2821 atccgttgcc ggcggagaaa gcccggcgtc gcgcatgcgc tgcggcaaca ggaccatcac
     2881 cgcgcgcctt tccgcgcttt ccatacgatg ccaccgcgcg atctcgggca gcgtgcgccc
     2941 gcagccgagg cagagattgg ttctgggatc gatgatacag actgcgatgc acggcgtgtc
     3001 ctgggtcatt ccgaaagtgt gaggatattc cggcgctggc gcaagcgccg tcggctctaa
     3061 gctcctgtgc ctgcgctcat gattggtctg tgtcgcggcc ggctcctttc gtcggtcgcg
     3121 gggctggtgt cgctttccgg ctgcagcggc ggcgcctctt cggtgaccgg cgcaagagcc
     3181 gacatgacgc gctccggcgg aaaggtgacg atcacctcgg tgccgatgcg cagtttcgat
     3241 ttcaacgtga aggtgccgcc gtgcaggtcg atcaggcttt tggcgatcgg caggccaaga
     3301 cctgcgcctt gttcggctga tttgatcgag ttggagccct ggccgaaaga cgccaacacg
     3361 accgggattt cgtcttcggc gatgccggag ccggtgtcct tgacgctgag atactggccg
     3421 ccggaggcgg tccagccgac cttcagccag acttcgccgc cttgcggggt gaacttgatc
     3481 gaattggaca agagattgag cacgatctga cggatggcgc gctcgtcgct ccagatcttc
     3541 ggcatgctcc gctcgaacac ctcgtggatg gtgatgccgc ggctggaagc acggagcttc
     3601 atcaaatgat ggcaatcggc aaccacgttc gccaatgcca ccgcttcctc gttcagctca
     3661 tagcggccgg cttcgatgcg cgacaggtca aggatctcgt tgatcaggtt gagcagatgc
     3721 acacccgaat tatggatgtc ggccgaatat tccttgtaga cgggaacggc gtgcgcgcca
     3781 aaaatctcgc tcttcatcac ttcggagaag ccgaggatcg cgttcagcgg cgtgcgaagc
     3841 tcgtggctca tctgggcgag aaagcgtgac ttggcgacgt tggccgattc cgcgcggtgg
     3901 cgcgcctcgt ccgagataga tttggcctgc tcgagctcgc cgatcagcgc atctttttcc
     3961 gcccgcgcct caagcgtggc gagggtcgtc gaatgcagcc ggtgggcgag cagggcgaaa
     4021 tagccttccg ctgcgaccgc aagcgccgca agcacgtaat gatcgaacgt accgctcagc
     4081 acgaaattga gtgcgatcat caccgtgatc ggaacggtcg ccgcgagcgc tgcgatcggc
     4141 aggttggccg cgaccatgct cgagattgcg atgaccagca gcatcaggaa catcatcacc
     4201 gtatcggaga cggcctcgag
//
LOCUS       DATLJT010000748         4096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_277001,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000748 DATLJT010000000
VERSION     DATLJT010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277001"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VM910_17135"
     CDS             <1..1087
                     /locus_tag="VM910_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HVI64287.1"
                     /translation="AARAGILAVALCAYVLAALFAERRQHEVALAESEARLQEALTAG
                     AVTTFVWDVGTGSSQRSADAAQILGFDPRQTFTPNNFLSRVHPDDRERFKALVRGVIP
                     ERPAYTIAFRFTRPDGREVWLEETARAEFDALGRFVRLKGLTLDVTARKRFEGQQSLF
                     IAELDHHVKNLLTRVAAVAKDTREESGSLDAYIQALDRRIRSMADAHALLSQSRWDGV
                     DLAELVRRQLAPNGTDANTTIAGPHVTLPVAATEVLAMVLHELATNAATYGALSTPHG
                     RVEVNWNHGPGEGAANLSIAWREIGGPAVAASPDCRYGIRIIRDLIPQELGGSVDLAF
                     ASGGVCCKIEIPMIQGRPRGIIRSQVN"
     gene            1288..2145
                     /locus_tag="VM910_17140"
     CDS             1288..2145
                     /locus_tag="VM910_17140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64288.1"
                     /translation="MGTRTTLLRRRFLHLAAGAVALPALARIAHGQAYPSRPVRIIVP
                     FAPAGPNDIIARALGQWLAERLGQPFVIENRSGAASNVGTEAVVNAAPDGYTVLMVSS
                     PHAINATLYDKLHFEFRRDIAPVAAIMRVPNVMVVDRTFPARSVPDFISYAKANPDKL
                     NFASSGIGASNHMSGELFKIMTGITMTHVPYRSSGPALADLLGGRVQVMFDSIASTIE
                     HIKADRLRSLAVTSAPRSTLIPDTPVMSDFVPGYDVNNWFGLGMPSQTPAEVIDRLNT
                     AVNEGLAEV"
     gene            complement(2287..3252)
                     /locus_tag="VM910_17145"
     CDS             complement(2287..3252)
                     /locus_tag="VM910_17145"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64289.1"
                     /translation="MLRLMIALVIGLVACGMTAASAQTYPQRPVKFILPFGPAAGTDI
                     TARLLGEKLAARWGKPVVIENRPGGDGLVAINAFTSANDEHTLLFVPASTFTAHPYAH
                     EKLPYDAERDLLPITNVTTIVIALSAPTSLNAKTLGEFIALARAKPDTLNVAAAAGNS
                     DLILSAFIKTQGLPVSRVPYRDIQQAPTDLAEARIHLLMSSYATMLPLVQAGKVRVLA
                     VTSRKRVGIAADIPTVAEAGYPYLGLDGLIGMYGPRGMSMALRESIAADVRAVVEADP
                     TIASRLAVTGQVIDLRGPHEFAAGIKEIREQLATIAQTLGVKAAQ"
     gene            3562..3726
                     /locus_tag="VM910_17150"
     CDS             3562..3726
                     /locus_tag="VM910_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64290.1"
                     /translation="MMQRDQYNPKDALSPDRLSDLDQVMADAVEMKFLDAPLTKEQLA
                     EFFQIPPPGT"
     gene            3867..>4096
                     /locus_tag="VM910_17155"
     CDS             3867..>4096
                     /locus_tag="VM910_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HVI64291.1"
                     /translation="MLAGKSGAASATRRLPNREAALEQLYSDISAKNMFPFWATSTDV
                     AHDEIKQLMGTQKAVPHLWSYKSDIEPILFRAA"
BASE COUNT          795 a         1274 c         1275 g          752 t
ORIGIN      
        1 tgccgctcgc gccggcatcc ttgccgtcgc gctctgcgca tacgttctcg ccgcgctgtt
       61 cgccgagcgg cggcagcacg aagtcgcgct cgcggagagc gaagcccgct tgcaggaggc
      121 gctcacggca ggggcggtca ctacgttcgt atgggacgtc ggcacgggtt cgtcgcagcg
      181 cagcgccgac gccgcgcaga ttctcgggtt cgacccgcgg caaactttta ccccgaacaa
      241 ttttctttcg cgggtgcatc ccgacgaccg cgagcggttc aaggcgctcg tgcgcggtgt
      301 catccctgaa cgtcctgctt ataccatcgc gttccgcttc acgcgccccg acggccggga
      361 agtctggctc gaggaaacag caagggcgga attcgacgct ctggggcgat ttgtacgcct
      421 caaaggtctt actctcgatg tcaccgcgcg caagcgattt gagggccaac aaagcctgtt
      481 catcgccgag ctcgatcatc acgtcaagaa tctgctgacc cgcgttgctg ccgtcgccaa
      541 ggacacgcgc gaggagagcg gttcgctcga cgcgtacatt caagcactcg accggcgcat
      601 acgatccatg gccgatgccc acgcgctgct cagccagagt cgctgggatg gcgtcgatct
      661 cgccgaactc gtccgccgtc agcttgcgcc caacggaacg gacgcaaaca cgacgatcgc
      721 tgggccgcat gtgacgctgc cagtcgcggc gacggaggtg ctggcgatgg tgctgcacga
      781 gctggcgacg aacgccgcga catacggagc gctgtcgacc ccgcatgggc gggtggaagt
      841 gaactggaac cacggacctg gcgagggtgc ggcgaacctg tcgatcgcat ggcgggaaat
      901 cggcggcccc gccgttgcag cttcgcccga ctgcagatac ggcatcagga tcattcgcga
      961 tctcatcccg caagagctcg gcgggtcggt cgaccttgca ttcgcgtccg gcggtgtttg
     1021 ctgcaaaatc gagattccca tgattcaagg ccgtccgcga ggaatcattc ggtctcaagt
     1081 caattgagca cggttgcgac aagctgtgct gcgctggcgc gttcctggtg tggccgccga
     1141 acggccgccc gataccgctc ggagaagatc tccacctaat ctccaacaaa attcaagcgg
     1201 ggctaaagct cggcgtcagg cttgtcgtcg aacccgccgc tacgttcgtg ggccgcgaac
     1261 accgctggca tagacgtgga ggacgagatg gggacccgca cgacgcttct tcgccgccga
     1321 ttcctgcatt tggccgcggg cgccgtcgcg ctaccagctc tcgcgcggat cgcacacgga
     1381 caagcctatc cgtctcggcc ggtgcggatc atcgtcccgt ttgctcccgc cggccccaac
     1441 gacatcattg cccgcgcgct gggacaatgg ctggcggagc ggctgggcca gccttttgtc
     1501 atcgagaacc ggtccggcgc ggcaagcaat gtcggcacgg aggccgtcgt gaatgcggca
     1561 cccgacgggt acaccgtctt gatggtatcg tcgccgcacg cgatcaacgc gacgctctac
     1621 gacaagctcc atttcgagtt taggcgtgac atcgcgccgg tcgccgcgat catgcgcgtg
     1681 ccgaatgtga tggtggtcga ccggaccttt ccggctcgat cggtacccga tttcatcagc
     1741 tatgccaaag ccaacccgga caagctcaac ttcgcgtcgt caggaatcgg cgcgtccaac
     1801 cacatgtccg gggaattgtt caagatcatg accgggatca cgatgacgca cgttccctac
     1861 cgcagttccg ggcccgcgct ggccgatctt ttaggcggcc gggtgcaggt gatgttcgac
     1921 tcgatcgcct ccacgataga gcatatcaaa gccgacaggc tgcgctcgtt ggcggtgaca
     1981 tcggccccgc gatcaaccct tattccagat acgcccgtca tgagcgattt cgttcccggc
     2041 tatgacgtga acaactggtt tgggcttggc atgccgagcc agacgccggc ggaggtcatc
     2101 gacaggctca ataccgccgt gaacgaaggc ctcgcggaag tttgaaggcg cgcttagcgg
     2161 gcatgggggg aaccgcatcg cccggctcgc cctcggattt cggcaagctc attgctgtcg
     2221 aaaccgaaaa gtggggcaag gtcatccgca ctgcgaatat taagccggcg tgatgcgcct
     2281 gcactgtcac tgtgcggcct tgaccccgag agtttgcgcg atcgtggcga gctgttcgcg
     2341 gatctccttg atgccggcgg cgaactcgtg cgggccacgc aaatcgatga cttggccggt
     2401 cacagctagc cggcttgcaa tcgtcggatc ggcctcaact acggcgcgga cgtcggcggc
     2461 aatgctctcg cggagcgcca tcgacattcc gcgcggcccg tacatgccaa tcaaaccgtc
     2521 tagtccgaga tacgggtacc ctgcttcggc gacggttgga atgtcggcag cgattccgac
     2581 ccgcttgcgg ctggtcaccg ccaagaccct aacctttccg gcctgaacca gcggaagcat
     2641 ggtcgcgtag gacgacatga gaaggtggat gcgtgcttcg gccaggtcgg tcggggcttg
     2701 ctggatgtcg cggtacggca cccgggacac cggcaagccc tgggtcttga tgaaagccga
     2761 caagatcagg tcggaattgc cagcggcggc cgccacgttg agcgtatcgg gctttgcgcg
     2821 cgccagcgca atgaactcgc caagcgtctt cgcgttcaga gatgttggtg ccgaaagcgc
     2881 gatcacaatg gtcgtgacgt tcgtgatcgg aagcaggtcg cgctccgcat cgtaaggtag
     2941 tttttcgtgc gcatatgggt gcgccgtgaa ggtcgaagcc ggaacgaaca aaagcgtgtg
     3001 ctcgtcgttc gcactggtga atgcgttgat ggcgacgagg ccgtcgccgc ccggccggtt
     3061 ctcgatcacc accggcttgc cccagcgcgc tgcgagcttt tctccgagca gccgcgcggt
     3121 gatgtcggtc cccgcggcgg gaccaaacgg caggatgaac ttgaccgggc gttgcggata
     3181 ggtctgagcc gaggccgcag tcatgccaca ggcgaccaag ccgatcacga gcgcgatcat
     3241 caaccgcagc acgcaacgcc tcccgtctaa gaacggcaaa aactctgcat cacagtgaaa
     3301 tgggcggccc atgtccaaag ttgttagaga ctggttcaga ttagagtcgt gtttgcagac
     3361 ggtaccggca agacgaatgt caggattcaa atacctgagc ttagcgagcg tcaccatttc
     3421 cacaagtatc tcacttgcct cccgtcaggc catccctcct cagcgagatc tatgctcacc
     3481 tccacgaagc agcggtagcg agctgggtca caggcaatcc ggcagtacgc cgagacgatc
     3541 aagcagccgg aaagcctggt cgtgatgcag cgcgaccagt acaacccgaa ggatgcattg
     3601 tcgccagacc ggctctcgga cctcgatcag gtgatggcgg acgcagtgga gatgaagttc
     3661 ctcgatgccc cgctaacaaa ggagcaactc gcggagtttt tccagattcc gccgccggga
     3721 acgtgacggc atcgtccccc ccgccacccg gcgcgttctc acgtgcgcgg atcaagcgga
     3781 gtggatgcgc gtgcgattga ccaggcagcc gcctatgtta ccttgcagcg aatggttcac
     3841 tccagccgtg tgaaggagcg accgacatgt tggcaggcaa gagcggcgca gcgagcgcga
     3901 cacgtcgtct tccgaatcgg gaagcggctc tcgagcagct ttatagcgac atttccgcaa
     3961 agaacatgtt cccgttctgg gcgacgtcaa ccgacgtcgc gcatgatgaa atcaagcaat
     4021 tgatgggcac gcaaaaggcc gttccgcatc tgtggagcta caaaagcgat atcgaaccaa
     4081 ttctatttcg agcggc
//
LOCUS       DATLJT010000749         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1655625,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000749 DATLJT010000000
VERSION     DATLJT010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655625"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1084)
                     /locus_tag="VM910_17160"
     CDS             complement(185..1084)
                     /locus_tag="VM910_17160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64292.1"
                     /translation="MRRIGVLMNLPADDAEGQGRIAAFLQELQQLGWTDGRNVLIDRR
                     WAAGDAGRFQRYAEELVALAPDVILAAAVPSVQALQRATRTVPIVFANVTDPVASGFV
                     QSLARPGGNTTGFMQFEFGLSGKWLELLKQVAPDVTGAAVLRDPDVGSGTAQFAVIQA
                     MAPLLRVEVNPVNVRDPSEIRRAVEARSPNGGLIVTASAMAYTHRDLIITLAARHKLP
                     TLYFDRSFVAASGLISYGPDYTNQYRRAASYVDRILKGEKPADLPVQAPTKYELVINL
                     KTARALGLEVPPSVLARADEVIE"
     gene            1465..2715
                     /locus_tag="VM910_17165"
     CDS             1465..2715
                     /locus_tag="VM910_17165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HVI64293.1"
                     /translation="MKHPRRIFLHLAAGATALTYLYPGLICAAIAFFVTQSPARADII
                     TEWNQTAVTTLVQAQLPTGAPTRALAMLHAAMFEAVNSIDPQFHPYKASIDAPKGASA
                     AAAASSAAYRVLAAVLPAQASTLEGRHKVLTAGIPESADRAAGILIGEKAAAAILALR
                     ANDGADFSAAYTPVPGTGMYVPTSTGMMASPSLGKMQTFVLEKSSQFRPPPPPAIDSA
                     QCLRDLGEVKALGEKDSAIRTKAQTDIARYHAPPGFIVWNSIARHAVQAKALDLVNSA
                     RAMALVNFGISDAVSAIFDAKYTYNAWRPVTAIRASGSASGWTALLPEPMHPEYPCAH
                     CGVGAAASTVLQRLFGSGPFPFSATAAPDVAPRAFGRFREFEEEEAISRIYAGVHFRW
                     SNAVGEIVGKQVGEKVLEALKPKS"
BASE COUNT          507 a          870 c          836 g          512 t
ORIGIN      
        1 gcaatccgcg cgaagatatc ggtgcctccg ccgggcgcga atggcacaat cacgcgcacc
       61 ggccgtgtcg gataggcttg cgcccgtgcg acccgcgaca tcgccggcag cgcagcagcg
      121 ccggcggcca gatgcagaaa ttgccttcgg tggggaagtt tcatttcaac ctccctcctt
      181 cacatcattc gatcacctcg tcggcgcggg cgagcaccga cggcggcact tcgaggccga
      241 gcgccctggc agttttcagg ttgatcacca gctcgtactt ggtcggcgcc tgcaccggca
      301 ggtcggccgg tttctcaccc ttgaggatac gatccacgta gctggctgcg cgccggtact
      361 ggttggtgta atcaggccca taggagatca agccgctggc ggcgacgaag gagcggtcga
      421 agtagagcgt gggcagtttg tgccgggcgg ccagcgtgat gatcaggtcg cgatgcgtgt
      481 aagccattgc gctcgccgtc acgatcagac cgccattcgg agagcgtgct tcgacggcgc
      541 gtctgatctc gcttgggtcg cgcacgttga ccgggttcac ttctaccctg agcaacggcg
      601 ccatagcctg aatgacggca aactgggcgg tgccgcttcc tacgtcggga tcgcgaagga
      661 ctgccgctcc tgtcacgtcc ggcgcgactt gcttgagcag ctccagccat tttccgctta
      721 ggccgaattc gaactgcatg aagcctgtgg tgttgccacc cggccgtgcc aaactttgga
      781 cgaagccgga ggcgaccgga tcagtgacgt tcgcgaatac gatgggcacg gtgcgggtcg
      841 cccgctgcag tgcttgaacg ctggggacgg ccgcggccaa aataacgtcc ggcgcgagcg
      901 ccactagctc ctcggcatat ctctgaaaac gaccggcgtc gcccgctgcc cagcgacggt
      961 caatcagcac gttgcggccg tcggtccagc ccaattgctg cagctcctgc aggaacgccg
     1021 cgatgcggcc ctggccttcc gcatcgtccg cgggcaagtt catgagcacg ccgatgcgcc
     1081 gcatccgctc gccctgctgc gcgctcgccg cgagcggcca cgctgccgcg ccgccgacca
     1141 gcgtgatgaa ctcgcgtcgc ctcatgcgca cccctcgtcc ggcttagggc cgcacattac
     1201 caggccgttg ggaagaacgt cgctgtgcat cacagcaaaa attgcgcgct aatgtcgcag
     1261 atgagtcaaa ctcggtgtcg cgtggttggt gtagcctgat cttcgagccg cccccagcgg
     1321 gatatgccga agtccctttt ttggcggcaa gctgatgcca tcggcgggca tcccactcag
     1381 accatcattt gtgcggtcta tccacgcttg catcgcgcgc ccgcgccctt ttacgcccta
     1441 aagacgatcc ggaggaggac gccaatgaaa catccccgcc gtatattctt gcatctggcc
     1501 gcgggtgcta ccgcgctgac gtacctctat cctggactga tttgcgcagc gatcgcgttc
     1561 ttcgtgacgc aatctcccgc gcgcgcggac atcatcacag aatggaatca aaccgcagta
     1621 acaactctcg tgcaggctca actccccaca ggagcgccga ctcgagcgtt ggcgatgctc
     1681 catgccgcaa tgttcgaggc ggtcaactcg attgatcctc agttccaccc ctacaaggct
     1741 tccatcgacg cgccgaaggg agcttcggcg gctgcggccg catcgtcagc cgcctatcgc
     1801 gtactggcgg cggtattgcc cgcacaggca tcaacgctcg aaggacggca caaagtgctg
     1861 accgcaggaa tcccggagag cgcggatagg gccgccggaa tattgatcgg cgagaaagca
     1921 gccgcagcta tcttggcctt gcgcgcaaac gatggcgcgg acttctcggc cgcttacaca
     1981 cccgttcccg ggacaggcat gtacgtgccg acgtcgactg ggatgatggc ttcgccttct
     2041 ctgggcaaga tgcagacctt tgtattagag aaatccagcc agttccgtcc gccgcccccg
     2101 ccagccatcg acagcgccca gtgccttcgt gatctcggcg aagtgaaggc gctcggtgag
     2161 aaggacagcg cgattcgcac gaaggcgcag accgacatcg cgcggtacca cgcccctccg
     2221 ggatttattg tctggaacag tatcgcgcgg cacgctgtgc aagctaaggc gctcgacctg
     2281 gtcaattccg cacgggccat ggccctggtg aattttggga taagtgatgc ggtgagtgca
     2341 atcttcgacg cgaaatacac ctacaatgcg tggcgaccag taaccgcgat ccgcgcgagc
     2401 gggtcggctt ccggctggac ggctttgctg cctgagccga tgcaccctga atacccctgc
     2461 gcacactgcg gcgtcggtgc agccgcgtcg acagtcctgc aaagactatt cgggtctggt
     2521 ccatttccgt tctcggcaac cgccgcgcca gatgtagccc ccagggcatt tggcaggttc
     2581 cgcgaatttg aagaggaaga ggccatttcc cgaatctatg cgggcgtgca cttccgctgg
     2641 tctaatgccg tcggcgagat cgtcgggaag caggttggag agaaggtatt ggaagctctc
     2701 aagccgaaaa gctgatgatc aggcg
//
LOCUS       DATLJT010000750         3592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3033623,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000750 DATLJT010000000
VERSION     DATLJT010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033623"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VM910_17170"
     CDS             complement(<1..614)
                     /locus_tag="VM910_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI64294.1"
                     /translation="MQFYLDGYAPGDPDILAAAPGAENRPSVLPDTTDVLIVGTGPAG
                     MLLAAQLSTFPAIATRIIDRREGPLQVGQADGVACRTVEMFDAFGLSARLIRESYWVN
                     ETVFWRPSPDDPAKITRTGRVQDTEDGLSEFPHLIVNQARLLQYLQEYMARSPSRLVP
                     DFGWDFETLTVAREGEHPVSATMRNVATGAKKIVRAKYIVGCDGA"
     gene            complement(670..1005)
                     /locus_tag="VM910_17175"
     CDS             complement(670..1005)
                     /locus_tag="VM910_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64295.1"
                     /translation="MVATSFLLGALAIMALTLKLPLAALLATIALVGIGTSGTQMLIY
                     GSVANYFPGVAWCAGFGRLGGVGGRLIAAGLSIATIFFVLAGLALVDVLLTVPASKAA
                     TTQDLAVAN"
     gene            1357..2724
                     /locus_tag="VM910_17180"
     CDS             1357..2724
                     /locus_tag="VM910_17180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64296.1"
                     /translation="MASIAPVGQTAKIIDIKAFIDAQRITAFQWFLVAICFMIVVADG
                     MDVAIMGFVTPSILKEWDISRPAFGIVISAAPFGLVIGALVAGPSSDRFGRKPVLIVS
                     VLAFALLTLAAAYSKNPTEMAVLRLLAGMGMGAAMPNTTTLLSEYAPQRRRSLMITVM
                     FSGFTLGSALIGFAAGYMIPLYGWRSVMLLGGAVPLALVPIIVLFLPESARLLALRGA
                     PASRIAAMLGRVTGFRFSGQETFVSKEPPLETRKPIGVLFSSGYSVTTIALWVTYFMG
                     LMVVYLLTGWLPTLMGDAGVPITTAANVTAMFQIGGTIGAVMVGWLMDKAPRPSLVIA
                     ATYLGGALSILAVAQAGVLSAALAALVFAAGFCTSGAQTGLNAFAPGCYPTVARATGV
                     SWMLGMGRFGSIVGSIVGGILLSLGWGFAAILSLLAAPALCAAIAISMAHRVSTERAA
                     EAAAH"
     gene            2948..>3592
                     /locus_tag="VM910_17185"
     CDS             2948..>3592
                     /locus_tag="VM910_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64297.1"
                     /translation="MDVAHLGHLELLTPKPDESLRFFVDVMGMTESGRKGNSVYLHAW
                     DDYERYSLQLTASKTSGLGHAAFRARSPQALQRRVAALEASGAGIGWHEDELAHGPAY
                     RFHDPDGHVIEIYYETEWYQAPEGLRPALKNQAQRYPARGVNVRRLDHFNCLAVDVKA
                     NREFFEKNLGFRSTERIVLNDGTEAGMWLTATNKSYDFAYTREAHGIKGRFHHIT"
BASE COUNT          707 a         1096 c         1087 g          702 t
ORIGIN      
        1 gcgccgtcgc agcccacgat gtatttcgcg cgcacgatct tctttgcccc tgtcgcgacg
       61 ttgcgcatcg tcgcagaaac cggatgttcg ccctcgcgcg cgacagtcaa cgtttcgaaa
      121 tcccagccaa aatcgggcac cagccggctc ggcgacctcg ccatgtactc ctgcagatac
      181 tgcagcaggc gcgcctggtt gacgatcaaa tgcgggaact ccgacagccc gtcctcggta
      241 tcctgcacgc ggcccgtgcg cgtgatcttc gccggatcgt ccggcgacgg ccgccagaac
      301 acggtctcgt tgacccagta ggattcgcgg atcagccgtg cgctcaggcc gaacgcgtcg
      361 aacatctcaa cggtgcggca ggcgacgccg tccgcctggc cgacctgcag tgggccttcg
      421 cgacggtcga tgatgcgcgt cgcaatagcg gggaaggtcg agagctgtgc ggccagaagc
      481 atgccggccg ggccggtgcc aacgattaga acgtccgtcg tgtcaggcag tacggaaggc
      541 cggttctcgg cgcccggcgc cgccgcgaga atgtctggat cgccgggagc atagccgtca
      601 agatagaatt gcatcgaaat cctcccatcg cattcatctt gcacgcttag ttggagcagg
      661 gctcgcgtct caattggcga cggcgagatc ttgcgtcgtt gccgctttgc ttgccggcac
      721 cgtcaggagg acgtcgacaa gggcgaggcc ggccagcacg aagaagatcg tggcgatcga
      781 caggccggcc gcgatcaggc gtccgccgac gcctccgagc cgcccgaagc ccgcgcacca
      841 ggcgacgccg ggaaagtagt tggcgacgga gccgtagatt agcatctggg tcccgctggt
      901 gccaatgccg accaaagcaa tggtcgcaag gagcgcggca agcggcagct tcaacgtcag
      961 cgccatgatc gcgagcgcac ccagcaggaa actagtggca accaccggct tcgcgtcgac
     1021 ggtcggcgaa gcgcgcgacg ctgatgggcc gagcgccgca gcgccgtgca gaacctgata
     1081 ccggccaacg tctacttcgg cagaggccgg acgtcctgct ggaacgcgaa aggatcaaac
     1141 gcgacactat caaacagcga cgattgcaac atcattcgaa agccgcctaa actccaactc
     1201 caaaggaacc agagccgccg ttccaataca gctcggattg tcccaaagcg tttgaagatg
     1261 gacagtcagc tgatgaagat actcagccaa gaatgcgcgt taaagctcgg ctgatcggga
     1321 aaatgcaccg tcaatctgca gctcttggag gaaacgatgg ctagtatagc ccccgtgggg
     1381 caaacagcaa aaatcataga tattaaagct ttcatcgacg cccagaggat cacggccttt
     1441 cagtggttct tggttgcgat ctgcttcatg atcgttgtcg ccgatggcat ggatgtcgcc
     1501 ataatgggct tcgtcactcc atccattttg aaggaatggg acatctctcg gccggctttc
     1561 gggatcgtca tcagtgcagc gccattcggc ctggtcatcg gcgcgctcgt ggctggtcca
     1621 tcatccgacc gcttcggtcg caagcccgtc ctaatagttt cggtgctcgc cttcgcgttg
     1681 ctaacgctcg cggccgccta ctcgaagaac ccgaccgaga tggcagtcct gcgtctcctc
     1741 gctggtatgg gcatgggcgc agccatgccg aacacgacga ccctcctctc cgaatatgct
     1801 ccacagcgaa ggcgctcgct gatgatcacg gtgatgttca gcgggttcac cctcggctcg
     1861 gcgctgatcg gcttcgcggc cggctacatg atcccgctat atggttggcg ttcggtcatg
     1921 ctcctcggcg gtgccgttcc attggccctc gtgcccatca ttgtcctatt cctgccggaa
     1981 tcggcgcggc ttctcgcgct gcgcggcgcg ccagcgtccc ggatcgcagc gatgctcggc
     2041 cgcgtgacag gtttccgctt ctccggccag gaaaccttcg tatcgaaaga accaccgctc
     2101 gagacacgca agccgatcgg tgttctgttc tcgtccggct acagcgtcac gaccatcgcg
     2161 ctctgggtga cctactttat ggggctcatg gtggtctacc tgcttaccgg ctggctccca
     2221 accctcatgg gcgatgccgg cgtgccgatc acgacggctg caaacgtgac cgcgatgttc
     2281 cagatcggcg gcacaatcgg ggcggtcatg gtcggttggc tcatggataa agccccacga
     2341 cccagccttg tgatcgcggc tacctatctc ggcggcgccc tttccatcct ggcggtcgca
     2401 caggcgggcg tcctgtctgc ggcactcgcg gctctcgtct tcgcagcagg cttctgcacg
     2461 agtggcgcgc aaacgggcct caatgctttt gcgccgggtt gctatccgac ggtggcccgc
     2521 gcgacaggcg tgagctggat gctcggaatg ggacgttttg gaagcatcgt cggatcgata
     2581 gtcggcggca tcctgctcag tctcggttgg ggcttcgcag caatcctgtc gctgctcgca
     2641 gctccggcgc tttgtgcggc gatcgctatt tccatggctc accgtgtcag caccgagcgt
     2701 gcagcggaag cggccgcaca ctagtaagtg cggtcgtaag aagaggcggt gcgggccgat
     2761 gttgcatggg ttgggtaccg ccttgcggcg aaagggtgca gtcagccgca aatagcatcg
     2821 gccagctgta caagcgagta gcatggggcc aaagcacgcg catctattga acatgggaca
     2881 aacgccctga gcgctctacc agaaacatac gctagactag aggagcagac ccgtggaaga
     2941 accaattatg gatgtggccc atcttggcca tcttgagctt ctgacaccaa aacctgacga
     3001 gagtctgcgg ttttttgtcg acgtgatggg catgacagag agcgggcgga agggaaattc
     3061 tgtctatctg cacgcttggg atgattacga gcgctactcg ctgcaattga ccgcgtcgaa
     3121 aacttccggt ctcggccatg ccgccttccg agcccgcagc ccgcaagcac tgcaacgccg
     3181 cgtagcagca ctcgaagcgt caggtgcagg gattggctgg catgaagatg aattggccca
     3241 cgggcctgcc taccgttttc atgatcctga cggccatgtc atcgaaattt attatgagac
     3301 tgagtggtac caagcgccgg aagggcttcg gccggctctt aaaaaccagg ctcaacgcta
     3361 tcccgcgcgg ggcgtcaatg tgcgtcggct cgatcatttc aactgtcttg ccgtcgacgt
     3421 gaaggctaac cgggagttct tcgaaaaaaa tctcgggttc cgttcgaccg agcgcattgt
     3481 gctgaatgac ggtactgaag ctggcatgtg gctaacggcg acgaacaaaa gctacgattt
     3541 cgcttacacc agggaagctc acggcattaa gggccgcttt caccacatta ct
//
LOCUS       DATLJT010000751         3295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3493970,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000751 DATLJT010000000
VERSION     DATLJT010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493970"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VM910_17190"
     CDS             complement(<1..668)
                     /locus_tag="VM910_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI64298.1"
                     /translation="MALEAPSSSSSSLFSPWPDRLRHSAIILLVAGLALSLLVLFDEL
                     SPLRAGAVFVCIAAAALVPWRLHHAVASREDVRGVNPVETPAVSAVVAGMPDPAVLLD
                     RAGRVIHLNAAAAQLAPALRKNELAQFALRSPEIITALREAIATSEPRRCSYLDHAPV
                     DRWMELIITPVPVPTVFGGVDKCMLMTFHDQTPLRRVEEMRADFVANASHELRTPLAA
                     LSGF"
     gene            complement(690..1748)
                     /locus_tag="VM910_17195"
     CDS             complement(690..1748)
                     /locus_tag="VM910_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HVI64299.1"
                     /translation="MLEATRRTLSFLRQKQVLHRLGVVISVTVIGIACYVLYHMLRGI
                     DVDEVIEAIKGTEPRQIALAALFVAAGYFTLTFYDLFAVRAIGRSNVPYRVNALAAFT
                     SYSIGHNVGASVFTGGAVRYRIYSAYGLNAIDVAKICFLAGLTFWLGNAAVLGLGIAY
                     HPEAAASIDQLPVWFNRTVAIGIIVALVAYVVWVWTQPRAVGRGPWTVVLPGGPLTLL
                     QIAIGIVDLGFCALAMYVLVPDEPNLGFVVVAVIFVSATLLGFASHSPGGLGVFDAAM
                     LVGLWEMDREDLLAGMLLFRLLYYIAPFVISVILLTFREVILGTRSKRLRQAAALGLD
                     AGPPREAAYVRERGDKHG"
     gene            complement(2228..2524)
                     /locus_tag="VM910_17200"
     CDS             complement(2228..2524)
                     /locus_tag="VM910_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64300.1"
                     /translation="MAYLVTFHWGWLSGALLLGLAMGWIAVVHRGPGVSKPMARWLAL
                     LVAALVALSLARVVPGRFGYWLDLGLILFACYLAGCAVGSWLRAWVVMRSRPAA"
     gene            complement(2530..>3295)
                     /locus_tag="VM910_17205"
     CDS             complement(2530..>3295)
                     /locus_tag="VM910_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI64301.1"
                     /translation="KANDVKAPPYVFQAYKDPVAVTLTLSGNVPDNNIHAAIAQAASR
                     KFFGEKVVDNLKASVGAPSGFAAAVVPALGALSRVSTGTLVVTDREVKLSGDALYDAA
                     AAQIRAGLGKDFPQGFQFKPDISVKPASAPVDASVCQQLFSELLGKAKVRFESGRASI
                     DPDSTGLLDHLVEIALRCPNSNIEVAGHTDADGEDAANQALSEKRAQAVVDYLVKAGL
                     PASRFTAVGYGSTQPVAGNDTDDGKAQNRRIEFLVR"
BASE COUNT          692 a         1024 c         1074 g          505 t
ORIGIN      
        1 atgaagccgg agagcgctgc aagaggggtg cgcaattcat ggctggcatt ggcgacgaaa
       61 tcggcgcgca tttcctcgac ccggcgcagc ggcgtctggt cgtggaaggt catcagcatg
      121 catttgtcga cgccgccgaa cacggtgggc accggcaccg gcgtgatgat cagctccatc
      181 cagcggtcga ccggcgcgtg atcgagatag ctgcagcggc gcggttcgga ggtggcgatc
      241 gcctcgcgca gcgcggtgat gatttcaggc gagcgcagcg caaactgcgc gagttcgttc
      301 ttgcgcaagg cgggcgcaag ctgggccgcg gcggcattga ggtggatgac gcggccggca
      361 cggtcgagca gcaccgcggg atcaggcatg cccgcgacga cggcgctgac ggccggggtt
      421 tcgaccgggt tgacgccgcg gacgtcctcg cgcgaggcca ccgcgtggtg cagccgccac
      481 ggcaccagcg ccgcggcggc aatgcagaca aacaccgcac ccgcgcgcag cggcgacaat
      541 tcgtcgaaca ggacgaggag ggacagcgcc aggccggcga cgagcaggat aatggccgaa
      601 tgccgcagcc ggtcgggcca cggcgaaaac agtgaagacg acgatgagga aggagcttct
      661 agtgccatct ggccgtttta tctccccggt cagccgtgct tgtctccacg ctctcttacg
      721 taagcggcct cacgcggcgg accggcgtcg agcccaagcg ctgcggcctg acgcaggcgc
      781 ttcgatcggg tgccgagaat aacctcccga aacgtcagca agattacaga tatgacgaat
      841 ggcgcaatat aatagagcaa gcggaacaat aacataccgg cgagcaggtc ctcccggtcc
      901 atctcccaca gcccgaccag catggcagcg tcgaatacgc caaggccgcc cggggagtgg
      961 ctggcaaagc cgagcagcgt cgccgagacg aaaatgacgg cgaccacgac aaagccgaga
     1021 ttgggctcat cgggcaccag cacgtacatc gcaagcgcac agaagccgag gtcgacgatc
     1081 ccgattgcga tctgcaacag cgtcaacggg ccgcccggaa gcaccacggt ccaggggccg
     1141 cgcccgacgg ccctgggctg ggtccagacc cagaccacgt aagcgaccag ggcgacaatg
     1201 atcccgatcg ccaccgtgcg gttgaaccag accggcaatt gatcgatcga cgcggcggct
     1261 tccggatggt aggcgatgcc cagtcccagc acggcagcgt tgccgagcca gaaggtcaga
     1321 ccggccagaa agcagatctt ggcgacgtcg atcgcgttca gcccataggc ggaatagatg
     1381 cgatagcgca ccgcgccgcc ggtgaagacg ctggcgccga cattgtggcc gatcgaatag
     1441 ctggtgaagg cggcgagcgc gttgacgcgg tagggtacgt tgctgcggcc gatcgcgcgg
     1501 accgcgaaca ggtcatagaa ggtcagggta aaatagccgg cggcaacgaa cagcgcggcg
     1561 agcgcgatct ggcgcggctc ggtgcccttg atggcttcga tgacttcgtc aacatcgatg
     1621 ccgcggagca tgtggtagag cacatagcaa gcgatcccga tgaccgtgac gctgatcaca
     1681 actccgagtc tgtgcaggac ttgtttttgg cgcagaaacg agagcgttct gcgtgttgct
     1741 tccagcatct agacctcgat caccaaccgc accgccgtgg gcatgcccca gttccgacct
     1801 tccagtgcgg ttttggattt gacattcgcc aggaaactga agcgaacgtc aaacccgccg
     1861 cacggtgtgg ttgttcagca gtccgccgga cgtctgaacc aaagccacac tggaatcata
     1921 gaccagagcc agccgaaccc gaagttcgca atagaggccg ccgcacaatt ctgcgaactt
     1981 cgggttcggg acactgattg gacgtggtag cgcgttttgg gccggagtgg aattcgcgaa
     2041 gtgcgaataa aaatgcgccg cttcaatatt ttaggttgaa ttgatgacgg aggaatgcac
     2101 agttttgctg cattcgtgct gcttgctatg gattccgggc gccaaggagc gcgcacggcg
     2161 gaagcatttt gcctcaagcg gatgaggaaa agcgcagaca tgtcggcccc gaacgggatc
     2221 gggacgctca ggctgccggc cggctgcgca tgacgaccca ggcgcgcagc cacgagccga
     2281 cggcacagcc tgcaagatag caggcaaaca ggatcaaccc gagatcaagc caatagccaa
     2341 agcgcccggg aacgacgcgc gcgagcgaca gcgcgacgag ggcggcgacg agcaaggcaa
     2401 gccaacgcgc catcggtttc gaaaccccgg ggccgcgatg caccaccgcg atccatccca
     2461 tcgcgagccc aagcagcagc gcccccgaca gccagcccca atgaaacgtc accagatagg
     2521 ccatcgccgt cacctcacca gaaactcgat gcggcggttt tgcgccttgc cgtcgtcggt
     2581 atcgttgccg gcgaccggtt gcgtgctgcc gtagccgacc gcagtgaagc ggctcgccgg
     2641 caggccggcc ttgaccagat aatcgaccac cgcctgtgcg cgcttctcgg acaaggcctg
     2701 gttggccgcg tcctcaccgt cggcgtcggt atgtccggca acctcgatat tggagtttgg
     2761 gcagcgcagc gcgatttcga ccaaatgatc gagcagcccg gtcgaatccg gatcgatgct
     2821 ggcgcgcccg gactcgaagc ggaccttggc cttgccgagc agttcggaga acaattgctg
     2881 gcagaccgac gcatcgaccg gcgcggaagc gggcttcacc gagatatccg gcttgaactg
     2941 aaaaccttgc ggaaagtcct tgcccagccc ggcgcggatt tgcgcggcgg cggcatcgta
     3001 aagtgcatct cccgacagct tcacctcgcg atccgttacc accagcgtgc cggtcgagac
     3061 ccgcgacagc gcgccaagcg ccggcacgac ggccgcggca aagccggacg gcgcgccgac
     3121 gctcgccttc agattatcga ccaccttctc gccgaagaat tttcgcgaag cagcctgcgc
     3181 gattgcggcg tgaatgttgt tgtcaggcac gttgcccgac agcgtgagcg tcaccgcgac
     3241 gggatccttg taggcctgga acacgtaagg cggcgccttg acgtcgttgg ccttg
//
LOCUS       DATLJT010000752         1512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3493987,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000752 DATLJT010000000
VERSION     DATLJT010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3493987"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..>1512
                     /locus_tag="VM910_17210"
     CDS             396..>1512
                     /locus_tag="VM910_17210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HVI64302.1"
                     /translation="MPKVTSRAAAASKRSAPKPSRLKASRRKSLPKGATRKIARKRTG
                     MAGRHAAELERYRLALESMNLNTYDWDIANNVVYISPAMREAVGFGPDQPFSLENWHH
                     FVHPDDQPLYRAALVSLLKGESARFECEYRYRARDGSWRWIRQHGLAARGRNGRAIRM
                     VGAAGDITDLKQRERAVQSAQAEVEVAQRGVEQAREAMRLVLDNISDGVVLTDNNLDL
                     KFANRRFVEFLQIPPEIASPGRSGYDILRFQARRGDYGPVESKKDLERLIQERAAMLR
                     MPTIRRYERRMSNGRYVEASFRQLADGSVLGFYRDITARKKREEALAAAKEAAETARD
                     EAERARAATSAARNDVERAYQMTQSILDNLSDGVVLAD"
BASE COUNT          312 a          471 c          468 g          261 t
ORIGIN      
        1 gacggatcga ccacggctcc cacgggagcg tggacacgcg cgtaggtggc tgagcccagc
       61 catcctccga cgccggccaa gattaatgcg gcggcaacag caaacggaca gatcttgcgc
      121 atccgagttt ctcccgttcc attggctcag gcgcgccgga cggcattgcc gcgtccacca
      181 aacgccctgc atgcggcggc ctttgatcaa ttctctttta aggatgaccc gtatcgcacc
      241 gatgtcgcgg cggcgggcaa agtgtatcgt cggtatcgca gggggtcggt cgcggtttcc
      301 gatctccatt ctcgcgatac aaacgataca aacgcggcag gtcggcgata catcgccgat
      361 acaggccccc ggtagcatcg cactaggagt ggctcatgcc aaaagtgacg tcccgggctg
      421 ccgctgcatc caagcggtca gctcccaagc cttcacgtct gaaggcctcc agacggaagt
      481 cgttgccgaa gggggcgacg cgcaaaatcg cgcgcaagcg caccgggatg gcgggtcgcc
      541 atgcggcaga actcgagcgt tatcggctcg cgctcgagtc aatgaacctg aacacgtacg
      601 actgggacat cgcaaacaat gtcgtctata tctctcccgc gatgcgcgag gccgtgggct
      661 tcggcccgga tcagccgttc tcgctggaaa actggcacca tttcgtccat cccgatgatc
      721 aaccgctgta tcgggcggca ctggtgtctc tattgaaagg tgagagtgcg cggttcgagt
      781 gcgagtatcg ctatcgagct cgcgacggat catggcggtg gatccggcaa cacggtcttg
      841 ccgcccgtgg ccgtaatggt cgggcgatcc gcatggtcgg ggccgccggt gacatcaccg
      901 acctcaagca acgcgaacgt gctgtccaat ctgcccaagc cgaagttgag gttgcccagc
      961 gcggtgtcga gcaagcccgt gaagccatgc ggctcgtact cgacaatatt agcgacggcg
     1021 tggtcctgac cgacaacaat ctcgatttga aattcgccaa ccgccgtttt gtggagttcc
     1081 tgcagatccc tccggagatc gccagtcccg gccggtccgg ctacgacatc ctgcgcttcc
     1141 aagcgaggcg cggcgattac ggcccggtgg agagcaaaaa agacctcgag cggcttattc
     1201 aagaacgcgc cgccatgttg cgaatgccta ccatccgtcg ctacgaacgg cgcatgagca
     1261 atggccggta cgttgaagct agcttccggc aattggccga cgggagcgtg cttggattct
     1321 atcgcgacat caccgcgcgc aagaagcgcg aagaggcgct ggcggccgca aaagaagcgg
     1381 ccgaaaccgc gcgcgatgaa gccgaacggg cgcgggccgc aacgtcagcg gcgcgcaacg
     1441 acgtcgagcg cgcctatcag atgacacaga gcatcctcga caacctcagc gacggcgtgg
     1501 tcctggctga ca
//
LOCUS       DATLJT010000753         4881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_47392,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000753 DATLJT010000000
VERSION     DATLJT010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47392"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VM910_17215"
     CDS             complement(<1..551)
                     /locus_tag="VM910_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVI64303.1"
                     /translation="MPLRLIFMGTPDFSVPTLLDLAAHGHEIAAVYSRAPKPGGRGMK
                     LQPTPVEVEARRLGIPVLTPKTLKTPEALAEFRAHEADAAVVVAYGMILPQAVLDAPK
                     LGCYNLHASLLPRWRGAAPISRAIMAGDTETGVMVMKMDAGLDTGDVAMAERIAITDR
                     MTAADLHDALARLGADLMVRALAA"
     gene            complement(621..1148)
                     /gene="def"
                     /locus_tag="VM910_17220"
     CDS             complement(621..1148)
                     /gene="def"
                     /locus_tag="VM910_17220"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVI64304.1"
                     /translation="MALREIIILPDKQLRLVSKPILKITPEIRTLADDMFETMYAAPG
                     IGLAAIQVAQPVRLITMDLAKKSEDGEVDPQPRVFINPEILSSSEETSIYEEGCLSIP
                     EYYEEVERPARVRVRFTDLDGKVHEEDAEGLYATCIQHEIDHLNGVLFIDYLSKLKRD
                     RVQKKFSKAAKRAVE"
     gene            1318..2520
                     /gene="rmuC"
                     /locus_tag="VM910_17225"
     CDS             1318..2520
                     /gene="rmuC"
                     /locus_tag="VM910_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVI64305.1"
                     /translation="MNQILFVMDGWPVHLTDALIGFAVLALVLLLTITIVVARSGRRE
                     TAFALAQSARADELEERLNDMLRAQAEASGRVDAMTQALAGRQADMARAVNERLDSVT
                     HRVGQSMEQSTRNTMDSLRVLHERLGIIDNAHKNLTDLTTQVTTLRDVLANKQSRGAF
                     GQARMEAIVQDGLPKGSYEFQYTLSSGKRPDCVVFLPDQRPLCIDAKFPLEAMTALHD
                     ARTEDERKIATQRLRADVMKHVSDIADKYLIAGETQDTALMFVPSESVYAEIHDGFDD
                     VVQKAYRARVVLVSPSLLMLAIQVMQQILRDARMRDAADQIRTEVLNLGDDLERLRER
                     VLKLQKHFSDVNEDVRQILISADKIEKRAGRIEELDFSKEPAEAPRLVKSGAPELFPP
                     PRKLQAGE"
     gene            2599..2876
                     /locus_tag="VM910_17230"
                     /pseudo
     CDS             2599..2876
                     /locus_tag="VM910_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129472.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            2971..4347
                     /locus_tag="VM910_17235"
     CDS             2971..4347
                     /locus_tag="VM910_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HVI64306.1"
                     /translation="MTAPETLADTAVEKTEIAPAPSWRESLAVYLQPRVLIVMFLGFS
                     SGLPLALSGSTLLVWMREAGVDLGTIGLFALVGTPYTLKFLWAPLVDALHVPFFTHTF
                     GRRRGWLLFAQLLLIATILELALADPARSPLMMAIAALLVATASSTQDIVVDAFRVES
                     LPESEQAAGMAGYVAAYRIGMLVSTAGALFIVSGFESSGLSRGSAWMWGYVVMAALVL
                     IGTVTALVATEPEQSVRAEAATRTETAFARVIHAAIGAFSEFLTRKDALAALAFVVLF
                     KFTDAFSGSMTAPFVIDLGFSRNDYAAIVKGVGLAATLIGGFAGGFVARRYSLAASLW
                     IGGVLQAAANLSFSWLAVVGINQWALALAITAENFTSAIGTVIFVAYLSALCQNPLHT
                     ATQYALLTALAAVGRTYLSSGAGFIAKAVGWPLFFVICMLVAIPSLMLLGYLQKRGHF
                     DALGPVKV"
     gene            complement(4344..4733)
                     /locus_tag="VM910_17240"
     CDS             complement(4344..4733)
                     /locus_tag="VM910_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64307.1"
                     /translation="MMAVTCAASAAPAQQAQAPAKAGMPINTGDILSGELTAMRVRSG
                     ERGKRVATYQITSEPRRLPQPAGLCNLETGPETFQLITTSESQATQLKSFVGKEISVK
                     VDEVACAQDAGQMSEAVITKWSVVTKP"
     gene            4651..4836
                     /locus_tag="VM910_17245"
     CDS             4651..4836
                     /locus_tag="VM910_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64308.1"
                     /translation="MLIGMPAFAGACACWAGAADAAHVTAIIAAQAQRLANPGFVMLQ
                     MSVLGELEERALAHRRR"
     gene            complement(4801..>4881)
                     /locus_tag="VM910_17250"
     CDS             complement(4801..>4881)
                     /locus_tag="VM910_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HVI64309.1"
                     /translation="GVPVGGELDYLDEGTLSAAMRQRTLF"
BASE COUNT          880 a         1529 c         1502 g          970 t
ORIGIN      
        1 gcggcgagcg cccgcaccat cagatcggcc ccgagccgcg ccaacgcatc gtgcaggtcg
       61 gccgcagtca tccgatccgt gatcgcgatg cgctcggcca tcgccacatc gccggtatcg
      121 aggcccgcat ccattttcat gaccatgacg ccggtctcgg tatcgccagc catgatggcg
      181 cggctgatcg gcgcggcgcc ccgccagcgc ggcaagaggg aggcatgcag attgtagcag
      241 ccgagtttcg gcgcatcgag aacggcctgc ggcaggatca tgccgtaggc aacgacgacc
      301 gccgcatccg cctcatgcgc gcgaaactcg gccagcgctt ccggcgtctt caaggtcttg
      361 ggcgtaagca cgggaatgcc gagtcgccgc gcttcgacct caacgggtgt cggctgcagc
      421 ttcatgccac gcccgccagg cttcggcgcg cggctataga ccgcggcgat ctcatgaccg
      481 tgcgcggcga gatccaataa cgttggcacg gagaaatccg gcgtgcccat gaagatcaga
      541 cgaaggggca tgaggtggaa atgtcctgcg atgatcagtt tattccgtca tgaccgggca
      601 cgacggccga gattgcggcc tcactccacc gcgcgcttgg cggccttgga aaacttcttc
      661 tgcacgcgat cacgcttcag cttcgacaga tagtcgatga acaacacgcc attgagatgg
      721 tcgatctcgt gctggataca ggtggcataa agcccctccg cgtcctcctc atgcaccttg
      781 ccgtcgagat cggtgaaacg cacgcgcacc cgtgccggcc gctcgacctc ctcgtaatat
      841 tcggggatcg acaggcagcc ttcctcgtag atcgaggttt cctccgacga ggacaggatt
      901 tccggattga tgaagacccg cggctgtgga tcgacctcgc cgtcttcgct ctttttcgcc
      961 agatccatgg tgatcagccg cacgggttgc gcgacctgga tcgccgcaag gccaatgccc
     1021 ggggccgcgt acatggtctc gaacatgtcg tcggcaagcg tcctgatctc cggcgtaatc
     1081 ttcagaatcg gcttggagac cagccgcaac tgcttgtccg gcaggataat gatttctctt
     1141 aaggccatgg cgggcgattt aaggcgctcg tttgccgggg tcaatgcgac cgggaaccca
     1201 ttaacggcct gctaaccatg aattttaggc cgcggttaac caagaaaatt gacccgctat
     1261 taaccataaa tgttccctct tcgttcgcgc aagggcgcga atcggctaca gaggggcatg
     1321 aatcaaattc ttttcgtcat ggatggctgg cctgtccacc tcaccgacgc gctgatcggc
     1381 tttgccgtgc tcgcgcttgt gcttctcctc accatcacga tcgtcgtggc gcgatcgggc
     1441 aggcgcgaga ccgccttcgc gctcgcccag tcagcgcgcg ccgacgaact cgaagagcgc
     1501 ctgaacgaca tgttgcgtgc gcaagccgag gcttccggcc gggtcgatgc gatgacgcag
     1561 gcgctggccg gccggcaggc cgacatggcg cgcgcggtga acgagcggct ggattcggtc
     1621 actcatcgcg tcggccagtc gatggaacaa tcgacgcgca acaccatgga cagcttgcgc
     1681 gtgctgcatg aacggctcgg catcatcgac aatgcgcaca agaacctgac tgatctcacc
     1741 acccaggtca cgaccttgcg cgacgtgctc gccaacaaac aatcgcgcgg cgctttcggt
     1801 caggcgcgga tggaagcgat cgtgcaggac ggcctgccga agggctccta cgagtttcaa
     1861 tacacgcttt cgagcggaaa gcggccggat tgcgtggtgt ttctgcccga ccagcggccg
     1921 ctctgcattg acgcaaaatt tccgctggaa gcgatgaccg ccctgcacga cgcgcggacc
     1981 gaagacgagc ggaagatcgc aacccagcga ctgcgcgcag acgtgatgaa acatgtcagc
     2041 gacatcgcgg acaaatatct gatcgccggg gagacgcagg acaccgcgct gatgttcgtg
     2101 ccgtcggagt cggtctatgc ggaaattcac gacggcttcg acgatgtggt gcaaaaagcc
     2161 tatcgcgcgc gcgtagtgct ggtctcgccc tctttgttga tgctggcgat ccaggtgatg
     2221 cagcagatcc tgcgcgatgc gcggatgcgg gatgccgccg accagatccg caccgaggtg
     2281 ctcaatcttg gcgacgatct ggagcgcttg cgcgagcgcg tgttgaaact gcagaagcac
     2341 ttctccgacg tcaacgaaga cgtgcggcag atcctgatct cggccgataa gatcgagaag
     2401 cgcgccgggc gcattgagga actcgatttt tccaaggaac cggccgaagc gccgcggctg
     2461 gtcaaatccg gcgcgcccga gctgttccca ccgccccgca agcttcaggc gggggagtaa
     2521 ttgtttgcgg taccatctcc gccatgatta ctgtcatcgc ccggcttgag cgggcgatcc
     2581 agtgtcccga ggcggccgat gggagcacgc aactacgacg tctccattct cgccagccgc
     2641 attggcggaa cgctctatat cggaatcatc aacgacctcg tccgtcgtgt aggcgagcat
     2701 cgactgaaac tggttgaggg ttttacaaag aaatacgacg tggcgcggct cgtgtatttt
     2761 gcgcaattcg acgacccgga aaatgcaatc aaacgcgaaa agcggttgaa gaagtgaatc
     2821 gagcctggaa gatacgattg atcgaagaac acaatccgaa ctggaatgat ctttaagtgg
     2881 agatcgcagc aggcggcgga taggtctctg gaatgctggg tcgcccggtc aaaccgggcg
     2941 acgacagccg gataaagctc ggatgatgac atgaccgcac ccgaaactct cgccgatacg
     3001 gccgtcgaga agaccgaaat cgcgcctgcc ccctcctggc gcgagagcct cgcggtttac
     3061 ctgcagccgc gcgtcctgat cgtgatgttc ctcggctttt cctcgggcct gccgctggct
     3121 ttatcaggct cgacgctgct ggtctggatg cgcgaggcgg gtgtcgatct cggcaccatc
     3181 gggctgttcg cgctggtcgg tacgccctac acgctgaaat tcctgtgggc gccgctggtg
     3241 gatgcgctgc atgtgccgtt cttcacgcac acattcggcc gccgccgcgg ctggctcttg
     3301 ttcgcgcaac tgctgctgat cgcgacgatc cttgaactcg cgctcgccga ccccgcgcgc
     3361 tcgccgctga tgatggctat tgccgcgctg ctggtcgcca cggcttcctc gacccaggac
     3421 atcgtggttg atgcattccg cgtcgaaagc ctgccggaga gcgaacaggc cgcaggcatg
     3481 gccggttacg tcgccgccta ccgcatcggc atgctggttt cgaccgcagg cgcgctgttc
     3541 atcgtatccg gcttcgagag cagcggtctt tcgcgcggct cggcatggat gtggggctat
     3601 gtggtgatgg cggcgctggt cctgatcggc accgtgaccg cgctcgtcgc gaccgagcct
     3661 gagcaatccg tccgggccga agcagcaacc aggacggaga ccgcattcgc acgcgtcatc
     3721 catgctgcaa tcggcgcgtt ctcggagttc ctgacgcgca aggacgcgct cgccgcgctt
     3781 gctttcgtgg tgctgttcaa attcaccgac gccttctcag gctcgatgac cgcacccttt
     3841 gtcatcgacc tcggcttctc ccgcaacgat tatgcggcaa tcgtcaaggg cgtcgggctc
     3901 gccgcgacct tgatcggcgg tttcgccggc ggtttcgtgg cgcggcgcta ctcgcttgcc
     3961 gccagcctct ggattggcgg cgtgctgcag gcggccgcca atctgtcctt ctcatggctt
     4021 gccgttgtcg gcatcaacca atgggcgttg gcgcttgcga tcacggcgga aaatttcacc
     4081 agcgcgatcg gcaccgtgat cttcgtcgcg tacctgtcgg cgctgtgtca gaatccgcta
     4141 cacaccgcga cccaatacgc cctgctcacg gcgctcgctg ctgtcggccg cacctatctg
     4201 tcgtcaggcg cgggctttat cgccaaagcc gtgggctggc cgctgttctt cgtgatctgc
     4261 atgctggtcg cgattccgag cctgatgctg ctgggatacc tgcagaagcg cgggcatttc
     4321 gacgcgttgg ggccggtgaa agtctagggc tttgtcacca cgctccactt ggtgatcacg
     4381 gcttcgctca tctggccggc atcctgcgca caagcaacct catcgacttt taccgagatt
     4441 tccttgccga caaagctctt gagctgggtg gcctgggact cgctcgtggt gatgagctga
     4501 aaggtttccg gcccggtctc gagattgcag agtccggccg gctgcggcag ccggcgcggc
     4561 tcgctggtga tctgataggt tgcgacccgt ttgcctcttt ccccactccg tacccgcatg
     4621 gcggtgagtt cgcctgacag gatatcgccg gtgttgatcg gcatgccggc cttcgctggc
     4681 gcttgtgcct gttgggccgg cgccgccgac gccgcgcacg ttaccgccat catcgcggca
     4741 caagcccagc gtctggcgaa tcctggtttc gtcatgttgc aaatgtccgt tttgggtgag
     4801 ttagaagagc gtgcgctggc gcatcgccgc agatagcgta ccttcgtcca gataatcaag
     4861 ctcgccgccg accggaactc c
//
LOCUS       DATLJT010000754         1539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_507280,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000754 DATLJT010000000
VERSION     DATLJT010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507280"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..>1539
                     /locus_tag="VM910_17255"
     CDS             551..>1539
                     /locus_tag="VM910_17255"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64310.1"
                     /translation="MASYIRRRKFLATLGGAAAAWPLAARAQQPAMPVVGFVNAGSSD
                     APFAAAFRKGLNEAGYFEGQNVTVEYHWLEGQFDRLPALMADLVRRRVAVIATPAGTY
                     TAKVAKAATTAIPIVFGVAEDPVKLDLVASLARPGGNLTGINFFAAELTAKRLALLHE
                     LVPKAVRIAVLVNPANIEPTAAALRDLPEAARAMGLQIQVLNASTRSEIEAAFATLMR
                     DRAEALYLAGDTFHTSRRVQFATLAARDRVPTSCPNRDMVEAGLLMSYGTDRADMSRQ
                     VGAYTGQVLKGAQPADLPVLQATKFEFVINLQTARALGIEVPPTLLARADEVI"
BASE COUNT          289 a          478 c          487 g          285 t
ORIGIN      
        1 cgtcgccgat tggtggaaac gcatgcgcga acggccctct acccgtacag cgatcctcga
       61 ccgaatgacg gaagccgact gggggccgtt caagaggctt gagcccgatc cctggcccaa
      121 ggtgcaggac ctcttgaagg cggcgtgacc gaactagggc tcgcgctctc cacgctttag
      181 tttgtcggcg cgtggccgtc ctctcacggg atatcgcacg taatcgctat actggcgcgg
      241 aatggtgtcg attaatggca cttctcggac ctcggaagat ttccgagttt agtctgcaaa
      301 gcggaccaaa gcggacattg attaggtcgc tgtcacagac gctcacgcgc gaaatgacgg
      361 aaggggaacg gcgaatccgc cgctacgttc acggctgatt ttggggcacg ggtaatcggg
      421 agcgtctttc agatttttgc cgcgctgtcg gcggcggcag cgccctaagc gtgaagtagt
      481 cacagaccgc gcgatggaag cgtggtacca ttcctccatt gcatgaatga cccccaaccg
      541 gaggggtcac atggcaagct acatcagacg ccgaaaattc ttagccacgc tcggcggcgc
      601 ggcagccgcg tggccgctgg cggcgcgcgc gcagcagccg gcgatgccgg tggtcggatt
      661 tgtcaatgcc ggatcatccg atgcgccctt tgctgccgcg ttccgcaagg gcctcaacga
      721 agccggctac ttcgagggcc agaacgtaac ggtggagtac cactggctag agggccaatt
      781 cgatcgcctt ccagcgctca tggctgacct cgtgcgtcgt cgcgtggccg ttatcgccac
      841 gcccgcgggc acttacactg ctaaagtggc caaagctgcg accacggcga tcccgattgt
      901 cttcggcgtc gctgaagacc cggtcaagct tgatctggtc gccagcctcg cgcggccggg
      961 cggaaacctg acggggatca attttttcgc ggctgagttg actgcgaagc ggctggcgct
     1021 tttgcacgag ttggtgccca aggcggttcg gattgccgtg ctggtcaatc cggccaatat
     1081 cgagcccacc gcggccgcac tgcgagacct accggaagcc gcccgtgcaa tggggctgca
     1141 aattcaggtc ctcaacgcta gcaccaggag cgagatcgag gcggccttcg ccactctcat
     1201 gcgcgaccgc gccgaggccc tatacctcgc tggcgatacg ttccatacga gccggcgcgt
     1261 ccaatttgcg acgctcgcgg cgcgcgatcg ggtacctact agttgtccca accgtgacat
     1321 ggtcgaagcg ggcctgctga tgagctacgg gaccgatcgt gcggacatgt cgcgtcaggt
     1381 tggggcctac accggtcaag ttctcaaagg ggcacagccc gccgacctcc cggtgttgca
     1441 ggcgaccaaa ttcgagttcg taatcaacct gcagacggcc agggcgctcg gcatcgaagt
     1501 gccgccgacg ctactcgccc gcgccgacga ggtgatcga
//
LOCUS       DATLJT010000755         3434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_47617,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000755 DATLJT010000000
VERSION     DATLJT010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47617"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VM910_17260"
     CDS             complement(<1..234)
                     /locus_tag="VM910_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64311.1"
                     /translation="MHDALEKALNGSEYLRDTLIEKIKESATVETSSSGDWQVTTAEI
                     DGVPSVRIISVSVLDQENLKFTCELEVEAEVKLF"
     gene            complement(969..1178)
                     /locus_tag="VM910_17265"
     CDS             complement(969..1178)
                     /locus_tag="VM910_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64312.1"
                     /translation="MPRGQPPRRLRIGSPEPGDEQDGAWPRGRPEQMDARFVERLERA
                     IERGEERRQREHLNGCRCADRTSPN"
     gene            complement(1225..1470)
                     /locus_tag="VM910_17270"
     CDS             complement(1225..1470)
                     /locus_tag="VM910_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64313.1"
                     /translation="MKMALSLMLALVTTTALAERLPQPKPPGPGGSCPHGYIASGSYC
                     TPSSGAADAIAKLPNGTCPWGWIASGAYCLRNGRARP"
     gene            complement(1506..2120)
                     /locus_tag="VM910_17275"
     CDS             complement(1506..2120)
                     /locus_tag="VM910_17275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64314.1"
                     /translation="MKLPRRQFLQLAAGAPVLPALSRIAKAQAYPSRPVRMIVGFPPG
                     SAPDIVARLLGQQLSDRLGQPFVIDNRPGASSNIATEAAVHAAPDGYTLLVVSLANVF
                     NATLYDKLNFDFIRDIAPIASIDRQPFVLETHPSVPARTVPEFIAYAKANPGKINMAS
                     AGAGSAPHVLGELFKTLAGVDLLHVPYRGGRSAEITKQDQGLAA"
     gene            complement(2156..3133)
                     /locus_tag="VM910_17280"
     CDS             complement(2156..3133)
                     /locus_tag="VM910_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64315.1"
                     /translation="MKLPRRNFLHLAASAAALPAVSRIARAQAYPSRPVHIIVPFAAA
                     GAFDILARLIGQWLSERLGQPFVIENRPGGGGNIGTEVVVRALPDGYTLLMVGPPHAV
                     NATLYDKLNFNFVRDIAPVAGVFRGPYVIVVNPSVPAKSIPEFIAYAKANPGKINVAS
                     GGVGSAHHVNGELFKMMAGVNLVHVPYRGAGPALVDLLAGQVQVMFAFMSSSIEYVRA
                     GKLRALAVTTATRSDALPDIPTVGEFVPGYEASGWFGVGAPKATPAEIVDQLNKEINA
                     GLADPKMKARLADLGGTVLAGSPADFGKLIAEETEKWGKVIRAANIKAE"
     gene            complement(3158..>3434)
                     /locus_tag="VM910_17285"
     CDS             complement(3158..>3434)
                     /locus_tag="VM910_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64316.1"
                     /translation="NTLALGARLPTIHSLREYVEASGLMSYGPNWSDQWRRAADYVDK
                     ILRGAKPADLPIEQPTKFDLVVNLTTAKVLGLTIPESFLLRADELIE"
BASE COUNT          664 a         1064 c         1062 g          644 t
ORIGIN      
        1 gaaaagcttc acctctgcct ctacctcaag ctcacacgtg aatttcaggt tctcctgatc
       61 aagaacgctt accgagatga tcctgaccga cggcacgccg tcaatctcgg cggtagtcac
      121 ctgccaatcg ccggatgatg aagtttcaac agttgccgac tctttgatct tttcaattaa
      181 cgtgtcgcgt aagtactcgc tcccgttgag ggccttttcg agcgcgtcat gcaaatcctg
      241 tgaaaccgtt gcgcgagaat tatatcgttg atggactcag catggaaaag cggcccggat
      301 tcggaacagc atgcttggaa atcacgatct tcggagacaa tgtaggctgt cgcgcggttc
      361 tgctggcacc aaaggcgaac ggaggcgatt gatatggcat cgggaaactc agatttcttc
      421 ttggtactaa acgggggacg ctgagcgaag tagtcatcga gcacgccagt tagatcagaa
      481 atcaaaggaa tgttgatcgc ccgagtatcc tgcaagaact gatcaaaagc cgcttccaag
      541 gtgcctagag cggtgctttg atcgcgcaca cggtcgatgg ttgacgaaac cccaagctgt
      601 tgaagtaacc cgctatgttt gctcagcgag gcatttgctt ccgcaagcag ctctcgaagt
      661 tgggacctaa cttctcttgt tgtgacgtcc gtcactatca ggcgtagccc tgctgcgcta
      721 attcgacgag ccttgataga gatcgtccgc cccaatcgac gcgagccctt cgaaaagcct
      781 gcgtgtctat gaatacgaac tgtgtccgaa ggtgtcgacc gccgtgttcc ggcatctgtg
      841 ctctcgttca ggcaatgccc cgcattcttt cagatcataa ggccaaaaat tccggctggc
      901 aggcgtgaaa gcgctaaatc agccaaaggc gaacagcggg cgaacgggca agtgcccacg
      961 ggagtggatc agttcgggct cgttctgtct gcgcagcggc agccgttgag gtgctcgcgc
     1021 tgacgccgct cctccccgcg ctcgatcgcg cgctcgagcc gctcgacgaa ccgcgcgtcc
     1081 atctgctcag gccgcccgcg cggccatgcg ccatcctgct cgtcgcccgg ctcgggcgaa
     1141 ccgatgcgaa gacgacgcgg cggttgcccg cgcggcacgg cgatgaccct cgacgacaga
     1201 caaaaacaaa tgcgagcgcg aaggctaggg ccgcgcacgg ccgtttcgca agcagtaggc
     1261 cccgctcgca atccatcccc acgggcaggt gccgttcggc agcttggcga tggcgtccgc
     1321 agcgccggac gacggcgtgc agtaggagcc cgacgcgatg tagccgtgcg ggcacgagcc
     1381 gcccgggccc ggcggcttcg gttgcggcag ccgctcggcg agagccgttg tcgtgacgag
     1441 cgcgagcatg agggaaaggg ccattttcat tgggtcgatc tctgttcggg acactcttgg
     1501 gacactcacg ccgcgagccc ttgatcttgc ttggttattt ccgctgaccg gccgccgcga
     1561 tacggcacgt gcagcaggtc gacgcccgcc agcgtcttga acagctcgcc gagaacatgc
     1621 ggcgcgctcc ccgctccggc tgacgccatg ttgatcttac cgggattggc cttggcatag
     1681 gcgatgaact cgggaacggt ccgggccggt accgatggat gcgtctccag cacaaagggc
     1741 tgccggtcga tcgacgcaat gggcgcaatg tcgcggatga aatcgaaatt gagcttgtcg
     1801 tagagcgtcg cgttgaacac gttggccagg gagaccacga gcagcgtgta gccgtccggc
     1861 gctgcatgca cggcggcttc ggtcgcgatg ttgctgcttg ccccgggccg gttgtcgatg
     1921 acgaacggct ggccgagtcg atccgacaac tgctggccca gcagccgcgc cacgatgtcg
     1981 ggcgcgctgc cgggcggaaa accgacgatc atgcgcaccg gccgcgacgg ataggcttgc
     2041 gcctttgcga tccgtgatag ggccggcagt acgggggccc ccgcggcgag ttgcaagaat
     2101 tggcggcggg gaagtttcat gagcgtccct ctctgaaatg ccagtgacgc gacgctcatt
     2161 ccgccttgat gttggccgcg cggatgacct tgccccactt ctcggtttcc tcggcgatga
     2221 gcttgccaaa atcggcgggc gagcccgcaa gcacagtgcc gcccaggtcg gcaagccgcg
     2281 ccttcatctt aggatcggcg aggcccgcgt tgatctcctt gttgagctgg tcgacgatct
     2341 cggcaggcgt ggccttcggc gcacccacgc cgaaccaacc gctcgcctcg tagcccggca
     2401 cgaactcacc cacggtcggg atgtccggca gcgcgtccga gcgcgtcgcg gttgtcaccg
     2461 ccagcgcacg aagtttgcca gccctcacgt actcgatcga tgaggacatg aaggcgaaca
     2521 tgacctgcac ctgcccggcg agcagatcaa cgagcgcggg ccccgcgccg cgataaggca
     2581 cgtgcacgag gttcaccccg gccatcatct tgaacagttc gccgttaaca tggtgcgcgc
     2641 tcccgacacc gcccgacgcc acgttgatct tgccagggtt ggccttggcg tacgcgatga
     2701 actcggggat cgacttggcc ggaaccgacg ggttcaccac tatgacgtag ggacctcgga
     2761 agacgcccgc gaccggcgca atgtcgcgga cgaaattaaa attgagcttg tcgtagagcg
     2821 tcgcgttgac cgcgtgcggc ggaccgacca tgagcagcgt gtagccatcc gggagtgccc
     2881 tcaccaccac ttcggtgccg atgttgccgc caccgcccgg ccggttctcg atcacgaatg
     2941 gctggccaag cctttccgac agccattgac cgattagacg cgcgaggatg tcgaatgcgc
     3001 cggccgcagc aaaagggacg atgatgtgca ccggccgcga cggataggct tgcgcccgcg
     3061 cgatgcgcga gaccgccggc agcgcggcag cgctcgctgc cagatggaga aaatttcggc
     3121 ggggaagttt cattttgaac ctccctctcg cttcacatca ttcgatcaac tcatcggcgc
     3181 gcagcaggaa cgactccgga atcgtcaggc cgagcacctt ggcggtcgtc agattaacga
     3241 cgagatcgaa cttggtcggc tgctcgatcg gaagatcggc gggcttcgca ccgcgcagta
     3301 ttttgtcgac atagtcggcc gcgcgccgcc actggtccga ccaatttggt ccataggaca
     3361 tcagaccgct tgcttcgacg tactcccgaa ggctgtgtat cgtcggcaac cgcgcgccga
     3421 gcgccaaagt gttg
//
LOCUS       DATLJT010000756         2368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3263926,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000756 DATLJT010000000
VERSION     DATLJT010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263926"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1070..2362
                     /locus_tag="VM910_17290"
     CDS             1070..2362
                     /locus_tag="VM910_17290"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI64317.1"
                     /translation="MARRNLLAIWEEKDFEEPFFSYRLLRRSIFVCNSPDTVAYALVA
                     HNDNFARKTPQMRNALAPLIGDGLFISDGKTWAKRRRIITPIVHASRLPLFAPIMVET
                     ANETAERWSAWTRGHPIDALHEMAMLTAEIICRAVFGPRLGSEHATTIVVSFSEYQRL
                     IPQLDVISLLGLPDWLPRYYSPGIRRAARRIHEVLNDIIRQCEESGDASMIRLLLDAR
                     DAETGAALDRDALRNEAAVIFMAGHETTANSLAWTWFLLSQAPDAEARLHVELDRVLA
                     GRLPTLNDVPRLVFTRAVFEESIRLYPPVPFLGRQALQDEEIRGRIVPAGSLVVVVPW
                     LLHRHRHLWDKPDHFVPERFLPESARARQRYSYVPFSVGPRVCAGQAFGLTEAILCLA
                     TLAQRVRLRLAPGAVVEPVCRLTLRPGGGLPMLIEHRN"
BASE COUNT          421 a          727 c          725 g          495 t
ORIGIN      
        1 agggacaagg atcgcaacac agcatctctt cgaggcatac gctgcagact gcaaacagcg
       61 gggggtgcgc gcggtcaact atcaagagtt cggccgggcg tgtacccagg tgttcggact
      121 cgtccaaaaa tgcaacgcgg aattcaacca cgacgagtgg gggtacccaa aaggccccgt
      181 gcgtcggccg cacggatatg acgtaccgac gcccgaggtc tggcaggagc gcctcgatgc
      241 acggttggta tccagaagca aaaacccccg gaacaggcgc aatgacgggt gacgagccac
      301 gagtcttatg caggtgtcgg gcttgtccga cttgtccgac ctctcagccg caggtcggac
      361 agcgaagctg cccccgtcgt tattggtttt gtccgacttg tccgacttgt ccgacttaat
      421 ttcgtgagcg ccgccccgct ctgcacccgc cgagggtgct gcggcggcgc agcccgggca
      481 tcacgctggc actcccgggc ggagtttcgg ctagtgcgcc ttcgtagtgg atgtgtgcgc
      541 gccgcttggc cgtcctgccg gtgtcatgac gatgagcgct tcgaatgaga agcctcccag
      601 ggcgcagcgt gcaaacggct attttatcat acagctcttc ggtgctactg tcggcgctcc
      661 gtcgaaagcc caggcctgtc ggctgctcgg cacgcccgtg gataccgcgc ggggagactg
      721 ccaaaagagc ccacctcctc ggactgctcg tcttcgacgg aggaccacat ccgaatgtga
      781 gcagcgccat gctgaaactg ccgaggcgag tctctttcga cgagacagcc ggtcgtcagc
      841 ttgcggggat tgaggcgccg acacttttcc ggtcttggcg cgagtggtgt ttcgtgctcc
      901 tggagagtca gattgcgggc cgctcatggg gcgcgctttt tcgcccggcc ggaacgatca
      961 attctttcaa aatactgctg gtcgctcgaa agccatcgaa gtgagcgctt taattccccc
     1021 ttacccaaac cgtccccggg ctccgctcga ccggtttgcg ttgttgtgca tggcgcgccg
     1081 caatcttctc gcgatttggg aagagaagga ttttgaagaa ccattcttct catatcgcct
     1141 gctgaggcgc tcaattttcg tctgcaatag tcccgataca gtggcttacg cgttagttgc
     1201 acataacgac aacttcgcgc gaaagacacc ccagatgcgt aatgctctgg cgccgctgat
     1261 tggggatggt ctgtttatca gcgatggcaa gacctgggca aagcgacgcc ggatcatcac
     1321 gccgatcgtc catgcctccc gactgccgct gttcgcgcca attatggtgg aaactgccaa
     1381 tgagaccgcc gagcgctggt cggcgtggac caggggtcat ccgatcgacg ctttgcacga
     1441 aatggccatg ctgaccgcgg agataatctg ccgcgccgtt tttgggccgc gcttaggaag
     1501 cgagcatgcg actacgatcg ttgtttcgtt cagtgaatat caacgcctca ttccgcagct
     1561 tgatgtcata tcgctgctcg gattgccgga ttggctgccg cgttactatt cgccgggcat
     1621 ccgccgcgcc gcccgccgaa ttcacgaggt tctcaatgac atcatccggc aatgcgaaga
     1681 gagcggtgat gcctcgatga ttcgactgct gctcgacgcg cgcgatgccg agaccggcgc
     1741 cgcgctcgat cgagacgcgc tgcgcaatga ggcagcggtc atcttcatgg cgggccatga
     1801 gaccacggcg aactcactcg cctggacctg gtttctgttg tcacaagcgc ccgatgcgga
     1861 ggcgcggctc catgttgagc tcgaccgggt gctggccggg cggctgccga ctctgaacga
     1921 tgtgccgcgc cttgtcttca cacgcgccgt tttcgaggaa agtatacggc tttatccgcc
     1981 ggtcccgttc cttggccgcc aggcgcttca ggacgaagag atccgcggcc gcatcgtccc
     2041 agccggatcg ctagtcgtcg tggttccgtg gctgttacat cggcaccgcc atctttggga
     2101 caagcccgac cacttcgttc cagaacgttt tttgccagag agtgcccgcg cgcgccaacg
     2161 ctatagctac gtgccgttca gcgttggacc gcgcgtctgt gccggacagg cattcgggct
     2221 gacggaggcg attctgtgcc ttgccacctt ggcgcagcgc gttcggctac ggcttgcacc
     2281 cggggccgtt gtcgagccgg tatgccggct aaccctgcgc cccgggggtg gcctcccgat
     2341 gctgatcgag catcgcaatt agtctgct
//
LOCUS       DATLJT010000757         1684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1656214,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000757 DATLJT010000000
VERSION     DATLJT010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656214"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..>1684
                     /gene="yidC"
                     /locus_tag="VM910_17295"
     CDS             36..>1684
                     /gene="yidC"
                     /locus_tag="VM910_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HVI64318.1"
                     /translation="MNENSRNTIIAVILSGLVLIAWQYFYNVPQMEKQRAAQQAQAEL
                     QKSNPPAAPATSSSPPPQPGTAPSPAPAAAAAAPQVVSRETAIAATPRVKIDTPRLTG
                     SISLKGARIDDLSLVQYRETVDPHSPAIVLYSPSGSPNPYYAEFGWVGSSGSTVRLPD
                     QTTMWQQESTGALTPTSPVVLKYDNGEGLTFHRTIAVDDRYLFTIKDEVTNVGNAPVT
                     LYPFALISRHGTPPVSGYYILHEGLIGYLGEKGLQEYTYKKIDDEKTVNFKVTNAWLG
                     ITDKYWASALLPDTNAQLQARYSSNLVGNARTYQTDYLEDPQTIAIGGTASVNARLFA
                     GAKEAGVVGINFPIWGFDGYNKQLSLNHFDLLIDWGWFYFITKPMFLALDFFYRLFGN
                     FGISILLVTVIVKLLFFPLANKSYASMAKMKSVQPQLQALKERYPDDKVKQQQEMMEI
                     YKKEKINPIAGCLPVALQIPVFFSLYKVLFVTIEMRHAPFYGWIKDLSAPDPTNLFNL
                     FGLIPFDPTQIPFFGHYLWLGVWPIIMGITMWFQMKLNPTPP"
BASE COUNT          331 a          560 c          471 g          322 t
ORIGIN      
        1 cccgcaaacc ggaatgaatg acgagatttt gaccgatgaa cgagaacagt cgcaacacca
       61 ttatcgccgt cattctgtcc ggcctggtgc tgatcgcctg gcagtatttc tacaacgtgc
      121 cgcagatgga aaagcagcgc gccgcccagc aggcgcaggc ggaactgcag aaatccaatc
      181 ccccggctgc gcccgcaacg tcctcgagcc cgccgccgca accgggcacc gcgccgtcgc
      241 cagcgccggc cgcggctgcg gccgcgcctc aggtcgtcag ccgcgaaacc gcgattgccg
      301 caactcctcg cgtcaagatc gacacgccgc ggctgaccgg cagcatttcg ctgaagggcg
      361 cgcgcatcga cgacctgtcg ctggtgcagt atcgcgagac ggtggacccg cattcgcccg
      421 caatcgtgct gtattcgccc tccggcagcc ccaatcccta ttacgcggag ttcggctggg
      481 ttggctcatc cggctcgacg gtgcggctgc ccgaccagac cacgatgtgg cagcaggaaa
      541 gcaccggtgc gctgacgccg acctcgccgg tcgtgctgaa atatgacaat ggcgagggtc
      601 tcaccttcca ccgcaccatc gcggtcgacg accgctacct cttcaccatc aaggacgagg
      661 tcaccaatgt cggcaatgcg ccggtcacgc tctatccgtt cgcgctgatc tcgcgccatg
      721 gcaccccgcc ggtctcgggc tactacatcc tgcatgaggg gctgatcggc tatctcggcg
      781 agaagggcct gcaggaatac acctacaaga aaatcgacga cgagaagacg gtgaacttca
      841 aggtcaccaa cgcctggctc ggcatcaccg acaaatactg ggcctcggcg ctgctccccg
      901 acaccaatgc gcaacttcag gcgcgctatt cctcgaacct cgtcggcaat gcccgcacct
      961 accagaccga ttatctggaa gatccgcaga ccatcgcgat cggcgggacc gccagcgtga
     1021 atgcgcggct gttcgccggc gccaaggagg ccggcgtcgt cggcatcaac ttcccgatct
     1081 ggggatttga cggctacaac aagcagctct cgctcaacca tttcgatctg ttgatcgact
     1141 ggggctggtt ctacttcatc accaagccga tgttcctggc gctcgacttc ttctatcggc
     1201 tgttcggcaa tttcggcatc tcgatcctgc ttgtcacggt tatcgtcaag ctcttgttct
     1261 tcccgctcgc caacaagtcc tacgcctcga tggcgaagat gaagtcggtg cagccgcagc
     1321 ttcaggcgct caaggagcgc tatccggacg acaaggtgaa gcagcagcag gagatgatgg
     1381 agatctacaa gaaggagaag atcaatccga tcgccgggtg tctcccggtt gccttgcaga
     1441 ttccggtgtt cttctcgctc tacaaggtcc tgttcgtcac catcgagatg cggcacgcgc
     1501 cgttctacgg ctggatcaag gacctctcgg cgccggatcc gaccaatctg ttcaacctgt
     1561 tcgggctcat tccattcgat ccgacccaga tcccgttctt cggccattat ctgtggctcg
     1621 gcgtctggcc gatcatcatg ggcatcacga tgtggttcca gatgaagctg aacccgacgc
     1681 cgcc
//
LOCUS       DATLJT010000758         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1656343,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000758 DATLJT010000000
VERSION     DATLJT010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656343"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1126)
                     /locus_tag="VM910_17300"
     CDS             complement(86..1126)
                     /locus_tag="VM910_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI64319.1"
                     /translation="MSSAFRAVQWNRTKLVYDGILLAGVALYISAYLILVYWLDPPKD
                     WPAAIDLRIRAFGSCAFLMLTIILSIGPLARLDRRFLPLLYNRRHFGVLTFFVALLHA
                     SFTVEWFAVQNALPSLYDELTKVSDYGKFIGFPFKTLGIVTLLILFLMAATSHDYWLV
                     FLTPSVWKGLHMALYAAYGLVVMHVALGVMQNDRNPFIPAMLAGGFAIVTLLHLVVGW
                     RERVRDRASARGSEGWLAVGPPLSIPDKSARIVTPPDGERIAVFRDGTQIAALSNLCA
                     HQNGPIGEGRIIDGCVTCPWHGYQYRLEDGCAPPPFTEKLVTYRVRIAHGVVEVDPRP
                     LPPGTAAAMSYE"
     gene            complement(1263..1457)
                     /locus_tag="VM910_17305"
     CDS             complement(1263..1457)
                     /locus_tag="VM910_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64320.1"
                     /translation="MIMLLAVTLYATAGTVIAAAFLAFGVTRVLPAPAAVTLGARIML
                     FPGAVALWPYVLIRWLRSSR"
     gene            1904..2566
                     /locus_tag="VM910_17310"
     CDS             1904..2566
                     /locus_tag="VM910_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVI64321.1"
                     /translation="MSMFLRRAVGCLAVATSLVLTLMGPSSAQSQGPEGLIRDPKQLV
                     QSLRSGGYVIVVRHGATFSDQADTNPFNFNDIAKQRNLNEKGKELAKAFGEAIRQVGI
                     PVGEVYTSNFNRAFETAVLAGFKDVKKTTDLTEGGLVVSPDENSRRAASLRNMLARAP
                     EKGKNAFLITHKPNIIDALGKEWFDVKEGEASIFKPEGAKYQLVARVQMEDWPKLAVA
                     VQ"
     gene            2878..>3226
                     /locus_tag="VM910_17315"
     CDS             2878..>3226
                     /locus_tag="VM910_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64322.1"
                     /translation="MSRKAAAALAVLLVLGTASAVRADADDRSAASRLAVIRSAPFCR
                     DGIAGRSRAWIKLTEPGGKLIHINVQHVTSVRSDTQIPGARAQLDLTSGKFQGVQETI
                     DEIMALISPAPGAR"
BASE COUNT          699 a          974 c          994 g          559 t
ORIGIN      
        1 atgccgcgaa gtggcaccca atccgaacca cgcatgatca tcccactcat ggctctcttc
       61 cttttccatc tgcatcgcgc tcatgtcact cataactcat cgccgccgcc gtacccggcg
      121 gcagcgggcg cgggtcgacc tcgaccacgc catgggcaat gcgcacgcgg taggtgacga
      181 gcttctcggt gaacggcggc ggcgcgcagc cgtcctccag ccggtactgg tagccgtgcc
      241 acgggcaggt cacgcagccg tcgatgatgc gcccctcccc gatcggccca ttctggtggg
      301 cgcaaagatt ggaaagcgcc gcgatctgtg tcccgtcgcg gaagacggca atgcgttcgc
      361 cgtcgggcgg agtgacaatt cgcgcgctct tgtccgggat cgacaacggt ggcccgactg
      421 cgagccagcc ttcgctgccc cgagcgctgg cgcgatcgcg cacccgctcg cgccagccga
      481 ctacgagatg gagcagcgtc acgatggcaa agccgccagc cagcattgcc gggatgaacg
      541 gattgcggtc gttctgcatg acgccaagcg ccacatgcat cacgacgagc ccataggcgg
      601 cgtaaagcgc catgtgcagt cccttccaga ccgacggcgt caggaagacg agccaatagt
      661 catggctggt cgccgccatc aggaacagga tgaggagcgt gacaatgcct agggtcttga
      721 agggaaaacc gatgaacttg ccgtagtcag aaaccttggt cagttcgtcg taaaggctcg
      781 gcaacgcgtt ttggaccgcg aaccattcga ccgtgaacga agcgtgcaag agggcgacga
      841 aaaaggtcaa cacgccgaaa tgacgccgat tgtacagcag cggcaggaaa cggcgatcaa
      901 gccgcgccag aggcccgatc gaaagaataa tcgtcagcat taaaaatgcg cagctgccga
      961 aagcacggat gcgcagatcg atcgcggccg gccaatcctt aggtggatcg agccaatata
     1021 ccaggatcaa ataagcgctg atgtagagcg ctacgcctgc gagcaagatg ccgtcgtaaa
     1081 cgagcttcgt gcggttccac tgcacggcgc gaaatgcgga actcatggtc tgacctctcc
     1141 cgctgcccct ggagcttcca cgttaggcgg tggccgcaac gtcagcgcca tggcgaagcc
     1201 caacgcgacc gccaaggcta ggatgggcca gagcgcgcgg tgaaccgatc gatgcgaccg
     1261 cgtcatcggg aggatctcaa ccaacggatg agcacgtagg gccagagcgc gacggcgccg
     1321 ggaaaaagca tgatccgcgc gcccagcgtt actgcggcgg gtgcgggcag cacgcgcgtg
     1381 acgccgaacg cgaggaacgc ggctgcgatc acagtgcccg ccgtggcgta gagcgtaaca
     1441 gccagcagca tgatcatgac accagctccg cgcgactgga gtgataggca atgatgtgcc
     1501 cgcgattgct gtagcgatag ggcgcgcgtg ggaacggcag gccgggaagc ggcatcgcga
     1561 aagtgttcag aactagataa gccgtctcca tagcaccgac ctctatcgag cgagagtcct
     1621 cccgacgtcg gccgacctgc cggccggcgc cgcccgctcc gacgagcaag agcggcgatc
     1681 gggaagattt attccttggt ctcttcgaga tttggtttgc tcgagagctt ctgtaacagg
     1741 cgatccaatt cgccgatttg acgggccgcc atgcaccgct cgtatctgat cacgattaat
     1801 tcaactgcgg aatacgattt gcttgatgcg agtcattacg cgcggacacg agttcgtctt
     1861 gatgacggcg cctcgtggcg acatgtcgta acggagaaat gagatgagca tgtttttaag
     1921 aagagccgtt ggctgtctgg ctgtggctac cagcctggtg ttgaccctga tgggaccgtc
     1981 ttcggcgcaa tcccaggggc cggagggttt gatccgagac cccaaacagc tagtccagtc
     2041 gctgcgttcg ggtggatacg ttattgttgt ccgacatggc gcaacattct cggatcaagc
     2101 tgataccaac cccttcaatt tcaatgacat cgccaagcag cggaatctca acgaaaaggg
     2161 caaggagctg gctaaagcgt ttggcgaagc aatccgtcag gtcggcatcc ctgttggtga
     2221 agtctacacg agcaatttca atcgcgcttt tgagacggcg gtccttgccg gatttaagga
     2281 cgtcaagaag acaaccgacc tgactgaggg gggtctcgtc gtatcgcccg acgaaaatag
     2341 tcggcgagcc gcatcccttc gcaatatgct ggcgcgcgcg cccgaaaagg gcaaaaacgc
     2401 tttcctgatc acgcataagc ccaacatcat cgacgccctc gggaaggagt ggttcgatgt
     2461 gaaggaaggc gaagcgtcga ttttcaaacc cgagggtgcc aagtatcagc tggtcgcgcg
     2521 cgtgcagatg gaggactggc cgaagctcgc cgttgccgtc cagtgacggg gcgccctctt
     2581 ggaagcggcg caggccgcac cagaatcgaa agcacccgcg cacgcgtcgc ggcgcctcaa
     2641 ttgatcgaat tgcattcgat accctccagc cagggccgct ttgcaggata tcgaatgggc
     2701 agggatcact cagcgggtgc ctcccgagcc aagtgcgcgt tgccccccaa gcgggacatc
     2761 gcgagctcgg ccgtgtgatg accatcacga aaaagcgctt ttcaccgctg cgccggaata
     2821 agcagcggcc tgatgccatc tcatgggacg aaggcgctcc gagcaaggag aacgaaaatg
     2881 tcccgcaagg ccgccgcggc tctggccgtc ctgctcgttc ttggcaccgc ctcggccgtt
     2941 cgagcggatg ccgatgaccg gtccgccgcc tcacggctcg ccgtcatccg cagcgccccg
     3001 ttctgccgtg atggtatcgc gggcagatcg agggcttgga tcaaattgac tgaacccggc
     3061 ggcaaactca ttcatatcaa cgtgcaacac gtcacgtcgg tcagatccga cacccaaatt
     3121 cccggcgcca gggctcaact cgacttgacc agcgggaaat ttcagggcgt gcaggaaacg
     3181 atcgatgaga tcatggcgtt gatctcgccc gccccgggcg cgcgcg
//
LOCUS       DATLJT010000759         1597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4413000,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000759 DATLJT010000000
VERSION     DATLJT010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4413000"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VM910_17320"
     CDS             complement(<1..654)
                     /locus_tag="VM910_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVI64323.1"
                     /translation="MSDEFKDNDQIKAGSKHHDQHAIDRRNLLLGTSTLVAAATLTSP
                     ALAQAQKAAPAAAPAPAAASGSKPNILVIMGDDVGWFNVGAYHQGIMSGKTPNLDKLA
                     AGGMRFTDYYAEASCTAGRANFITGQLPLRSGLTTVGQAGADVGMPDQCVTIATVLKS
                     LGYATGQFGKNHLGDLNKYLPTLHGFDEFFGYLYHLDAMSDPYWFDYPQDWIDKYGPR
                     "
     gene            complement(949..>1597)
                     /locus_tag="VM910_17325"
     CDS             complement(949..>1597)
                     /locus_tag="VM910_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64324.1"
                     /translation="TVTLIDDDQWTVAGDFDSDRPLYRIAVNDAAGTEIYVSSAKGDV
                     VLFTTRSMRLANYFGSIAHWIYPTVLRRHGKAWSALMWWLSLVATIGASIGVIVGIVR
                     LGAATAYRGLQRAHHTFGLIFAPFILSWIFSGFLSVDDGWLFSPSQSRIISAGPVDLF
                     RKLHTFDFAPLTSHPRLRSSLVVGLCLCGLAFSLTGVVLAWRRLRLGAGRRKNVV"
BASE COUNT          319 a          470 c          509 g          299 t
ORIGIN      
        1 gcgcggacca tacttgtcga tccagtcctg cggatagtcg aaccagtaag gatcggacat
       61 cgcgtcgagg tgatacaggt agccaaagaa ttcgtcgaag ccgtgcagcg tcggcagata
      121 tttgttcagg tcgccgaggt gattcttgcc gaactgtccg gtggcgtagc cgagcgattt
      181 gagcacggtc gcgatcgtca cgcactgatc cggcatgccg acatccgcac cagcctggcc
      241 aacggtggtg agcccgctgc gcaggggaag ttgcccggtg atgaaattgg cgcggcctgc
      301 ggtgcagctt gcttcggcgt agtagtcggt aaagcgcatg cccccggcgg cgagcttgtc
      361 gaggttcggc gttttgccgg acatgatgcc ctggtgatag gcgccgacat tgaaccagcc
      421 aacgtcgtcg cccatgatga cgaggatgtt cggcttgctg ccggaagcgg cggcaggcgc
      481 gggtgccgcg gcgggcgcag ccttctgggc ctgcgccaat gcgggtgagg tcagcgttgc
      541 cgctgccacc agcgtcgacg tgccgagcag aagattgcgg cgatcgatgg cgtgttgatc
      601 gtgatgtttc gagccggcct tgatctggtc gttatccttg aactcatcgc tcatttgaca
      661 ctcctcgttg cacgaaaata cctacggccg cgttccgacg acgcacctac atggctcatc
      721 gacgtgttga ccggctcggc atggcgggcc ggccggtcga taacggtggc agtagtgcgc
      781 ggcgcacacg aatgtgagcc gcccttcaat accttgcggg ggatacggat aacggcttga
      841 catggatcaa ggctctgggt ttcgggcccg ccacgcggat tttgccggat acggcacgct
      901 ttcggcgccg gcctttcgcg gattctacct tttcaaacga cattcttttc aaacaacatt
      961 tttccttcgg cctgcgccca agcgcagcct tcgccacgca agcaccacgc ccgtaaggct
     1021 gaaggcaagg ccgcacagac agaggccgac gaccaggctt gaacgcaacc gcgggtgcga
     1081 cgtcagcggc gcgaaatcga aagtgtgcag cttgcgaaac aggtcgacgg gcccggcaga
     1141 aatgatccgg ctctgacttg gggagaacag ccagccgtcg tccaccgaca ggaaacccga
     1201 gaatatccag gagaggatga atggtgcgaa gatcaggccg aaggtgtgat gcgcgcgctg
     1261 caggcctcga taggccgtgg ccgcgcccag tcgaacgatg ccgacgatca cgccaatact
     1321 cgcaccgatg gtcgccacca aggacagcca ccacatcagc gcgctccacg ccttgccgtg
     1381 acggcgcagc acggttggat agatccagtg cgcgatgctg ccgaagtaat tcgctagccg
     1441 catgcttcgt gtggtgaaga ggacaacgtc gcccttggcc gatgaaacgt agatctcggt
     1501 gcctgcagca tcattgacgg cgatgcgata cagcgggcga tcggaatcga aatcccccgc
     1561 aactgtccac tgatcgtcgt cgatcaaggt cacggtt
//
LOCUS       DATLJT010000760         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2575918,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000760 DATLJT010000000
VERSION     DATLJT010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575918"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VM910_17330"
     CDS             <1..198
                     /locus_tag="VM910_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64325.1"
                     /translation="GSAIRSSTPNVRSSCHPVLSFGANMGMAEFGTTSAAKPVPVVDK
                     IAIKHVKANFDMFPRRTELCP"
     gene            326..1702
                     /locus_tag="VM910_17335"
     CDS             326..1702
                     /locus_tag="VM910_17335"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HVI64326.1"
                     /translation="MNRTTWLQDRRMQKFRDVLSRWERKELSAIEAGELLGCSERQFR
                     RYRRRYEEEGLAGLVDRRLGKASMRRVPVDKLVWMLGEYRTHHMGWNVKHFHEHIRQQ
                     HGFGWGYTWTKTQLHTAGLVERAARRGAHRRKRPRKPCEGMMLHQDGSRFAWLSGAPE
                     LDLIVTMDDATSTIYSAFLVEEEGTVSTFRGLLEVFTAKGLPASLYTDRGSHYFYTPK
                     AGEAVDKSRLTQVGRALDRLGIEHIAAYSPEARGRSERMFGTLQDRLPKELKLAGIYE
                     VEAANRFIREVYVPAHNARFARPPQIAESGFVAADSGMLAEMLCIEVERVVGRDNTVS
                     HNGRRLQLPASPLRAHYVKASVKVREYPDGTLAVFHGPRRIARYDAEGAELLVPTAGS
                     LTPCSPSSRRGLVAGEFAASASRRPALTAAARGVPAPARVGTKKRLSGRTKKLTGKEL
                     RLTTSAPV"
     gene            1982..>2299
                     /locus_tag="VM910_17340"
     CDS             1982..>2299
                     /locus_tag="VM910_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64327.1"
                     /translation="MTTYDGLDRLDAAGITFITLRRRSPRLLTEIDRLSVSAWRIVNL
                     DISHRKYRTPRVYEQKVCPRKHTFRQFFIKDLGHEEPTILLTNDASSARNIIARYAKR
                     MLIE"
BASE COUNT          452 a          672 c          755 g          420 t
ORIGIN      
        1 ggcagcgcaa tcagatcgtc gacaccaaac gtgcggtcaa gctgccaccc ggtcctgtcg
       61 ttcggagcga acatgggaat ggccgaattc gggacgacat cggccgccaa gccggtgccc
      121 gtcgtcgaca agattgccat caagcacgtc aaagcaaatt tcgacatgtt tccccgcaga
      181 actgaactct gtccgtgact aggataaccg tactcagacg acggttcaat cggcaaaagc
      241 gctcgttgtc gtaagcagct gagttgtccg ggttatgtag cacacgagtt gtccggtttt
      301 attgcctccc caggggaggc gatcgatgaa ccggacgacc tggctacagg accgcaggat
      361 gcagaagttt cgggatgttt tgagccgctg ggagcgcaag gagctgtcgg cgatagaggc
      421 tggcgagctt ttggggtgct cggagcggca gttccggcgt taccggcggc gctacgaaga
      481 ggaggggctt gccgggttgg tcgatcgccg gctcggcaag gcgtcgatgc gtcgggtgcc
      541 ggtggacaag ctggtgtgga tgctcggcga ataccggacg catcacatgg gctggaacgt
      601 gaagcatttc cacgagcaca tccggcagca gcatgggttt gggtgggggt acacctggac
      661 gaagacgcag ctgcacacgg ccggtctggt ggagcgcgcg gcccggcgcg gggcgcatcg
      721 tcgcaaacga ccgcgcaagc cgtgcgaagg gatgatgctg catcaggatg gcagccggtt
      781 tgcgtggctc agcggggcgc ccgagctcga tctgatcgtc acgatggacg atgccaccag
      841 cacgatctac tcggcgttcc tggtggagga agaaggaacg gtttcgacct ttcgggggct
      901 gctggaggtg ttcacggcga agggccttcc ggcgagcctc tataccgatc gcggcagcca
      961 ttatttttac acgccgaagg cgggggaggc tgttgacaag agccggctca cccaggttgg
     1021 ccgcgcgctc gatcggcttg ggatcgagca tattgcggcc tattcgccgg aggcgcgcgg
     1081 gcgttcggag cggatgttcg ggacgctgca agatcggctg ccgaaggaac tcaagctcgc
     1141 cgggatttac gaggtcgagg cagccaatcg gttcatccgc gaggtttatg ttccggcgca
     1201 caacgcgcgc tttgccaggc cgccgcagat cgcggagagc ggtttcgtag ccgccgattc
     1261 cgggatgctt gctgagatgc tgtgcatcga ggtcgagcgc gtggtgggac gcgacaacac
     1321 cgtttcgcat aacggacggc gcttgcagct gccggcgagc ccactgcgtg cccactacgt
     1381 gaaagcctcg gtcaaggtgc gcgaatatcc ggacgggacg ctggcggtat tccacgggcc
     1441 acggcggatc gcgcgctacg acgcagaggg ggcggagctt ctcgtcccga ctgcgggcag
     1501 cctgacgccg tgctcaccgt catcaaggcg tggcctggtg gcgggagagt ttgcggcgag
     1561 cgcgtcgcga cggccagcct tgacggcggc tgcgcgcggc gtcccggcgc ccgcgcgggt
     1621 cgggacgaag aaacggctct cgggtcgaac caagaaactg acaggaaaag aactgaggct
     1681 caccacgtcg gccccagtct gatgcaagtc gcgcccgccg gggcgtccac cgcttccaaa
     1741 ccggcagggt cgatctcacc gcgaagcgga cagatgatgt gctacgaaaa ccggacaact
     1801 tgacgagcta ccgacaccca gcattcttac tttccttgcc caagatgccg agagccaagt
     1861 cttttgctat tcgaatgcgg atatccgcaa gggcgaggag gctgaggagg tctttcgttt
     1921 catccgcttc tggaagcgca agtacggcaa tgagccgaag catctggtct ttgattcgaa
     1981 attgaccacc tatgacggtc tcgatcggct cgacgccgcc gggatcacct tcatcacatt
     2041 gcgccggcgc tcaccgcgcc tgctcaccga gatcgatcgc ttgtcagtat cagcctggcg
     2101 catcgtcaat ctcgatatct ctcaccgcaa ataccgtact ccaagggtgt atgagcaaaa
     2161 ggtttgtccg cgcaagcaca cgttccgcca gttcttcatc aaggatctcg gccacgagga
     2221 gcccacgatc ctgctcacca acgacgcaag ctccgcgcgc aacatcatcg cccgctatgc
     2281 caaacgtatg ctcattgag
//
LOCUS       DATLJT010000761         3425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2115334,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000761 DATLJT010000000
VERSION     DATLJT010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115334"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VM910_17345"
     CDS             complement(<1..243)
                     /locus_tag="VM910_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HVI64328.1"
                     /translation="MNAKGIEAVRRILMVFPRYTSAFATWEYAYPLTDGVQALMPPQG
                     LLLIAAYLPKSWPVRFVDENIRAATREDFEWAEVVFV"
     gene            complement(460..1839)
                     /locus_tag="VM910_17350"
                     /pseudo
     CDS             complement(460..1839)
                     /locus_tag="VM910_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144019.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
     gene            2157..>3425
                     /locus_tag="VM910_17355"
     CDS             2157..>3425
                     /locus_tag="VM910_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVI64329.1"
                     /translation="MAIASRFSLAARLYSIFILFAILTAAITVLSDYNSRQGAELTDA
                     IEKANMAALNVERVNSLVYAVVMESRGIYMSTEPEVVKKYGEGLLKFTGNILAVVNRW
                     ESIVQADDAEQFAAFRKRIEQFVDFRKELVRRANEISPAAGREWGDNDANRSVRSALN
                     KDLETLSKVYVERGKQIAERAKLNRQLSFVLTCLGGVALLLVVFGVAIIGRSIARPLS
                     AITSTIKQVADGAENVVVPHTDRADEIGALARAIRIFQEAMERNRKLNSQVSADSKAR
                     EERSRHIEASVEAFRQAIGAVLRAVNDNALAMRNAAQTISRVTSDANERAVAAAGATT
                     QASGNVSAVAGATEELSTSVQEIGRQVRQSAGAVEQTGQRTEKSIAEIESLAAATQRI
                     DGVLQLIQAIAEQTNLLALNATIEAARAGDA"
BASE COUNT          686 a         1097 c         1043 g          599 t
ORIGIN      
        1 gacgaagacc acttccgccc attcgaaatc ttctcgggtc gcggcgcgta tattttcgtc
       61 gacgaagcgg accggccagc ttttcggcag ataggccgcg atcagcaaca ggccctgcgg
      121 cggcatcaag gcctgaacgc cgtccgtcag cggataggcg tattcccagg ttgcgaaagc
      181 ggacgtatag cgcgggaata ccatcaggat gcggcgtacc gcttcaatac ctttagcgtt
      241 catggacttt ctcaagcccc ctttggctca tgcccagccc acaccaacat gcgtctaaaa
      301 ccaaaccaaa tcaataagtt cccagtggtt ctggatcccg ttcgcaaacc aggatggcgc
      361 gaacatcgca atcgggacac caaaaatccc tcaacattgt gacgagccca acgtttgtga
      421 cgccggatcg gttgcagcgg tcccattttt agggacatcc gcgatcaaac gggacgcgaa
      481 atcgggcaga agccccgcat ttccagcaag gcggaagcct gcgtcggcca gtcccgtacg
      541 cacgagccaa tcatcgaact ccggcgcccg ccgcaatccg aagaggtcgc ggacgtagag
      601 cgcgtcgtgg aaggaggtcg attgatagtt caacatcacg tcgtcagcgc caggcacccc
      661 catgataaag gtgacgccgg ccgcggcgag cagcgtcaac aggttgtcca tgtcatcctg
      721 gtcggcctcg gcatgatttg tgtagcagac gtcgacgccg agcggcaggc cgagcagctt
      781 gccgcagaaa tggtcctcca gcccggcgcg gatgatctgc ttgccgtcgt agaggtattc
      841 cgggccgatg aagccgacca cactgttgac gagcagggga tcgaacgcac gcgccaccgc
      901 gtaggcgcgc gcctcgcaag tctgctggtc gacgccatgg tgcgcgttgg ccgagagcgc
      961 cgagccctgc ccggtctcga aatacatgac attctcgccg acggtcccgc gccggagcga
     1021 ctgtcccgcg gcccgcgcct cgcgcaggac ggcaagatcg atgccgaaac tgcggttggc
     1081 ggcctcggtg cccgcgaccg attgaaacac caggtcgacg ggcgcgccct cgccgatcag
     1141 tttcagcgtc gtcgtcacat gggtcaacac gcagccctgg gccggaatgt tcaggcgcgc
     1201 gatgatgtgg tcgagcaggc gcaagagctc gccgatcgtc gccggatcgt cgcttgccgg
     1261 attgatgccg atacaggcat cgcccgagcc cagcagaatg ccgtcgagga tcgaggccgt
     1321 gattcccttg gcatcgtcga agggatgatt gggctgcagc cgcgcactca tggtcccccg
     1381 cagcccgatc gtgttgcgga aagccgtgac cacctcgcat ttccttgcag ccaggatgag
     1441 gtcctgattg cgcatcagct ttgagaccgc agccgccatt tccgcggtga tgccggacga
     1501 cagccgcttc aaggcttgcg tggttgcggc atccgacagc aggaagtcgc ggaagccgcc
     1561 caccgtgaga tgcgcgatcg gggcaaaggc ccgcgcatca tggccgtcga tgatcagacg
     1621 agtgacctcg tcgtcgtcat agggaatcac ggtctcctgc aggaattgcg cgagcggaac
     1681 atctgcgagc gccattcgcg ccgcgatcat ccgctcggcg ctgtcggccg caatcccggc
     1741 caggcggtcg ccggaacgca agggcgtcgc cttggcgagc aggtcgcgga ggtcatcgaa
     1801 cgcgtaagag gtggcatcga tcgtgtggcg atagaccatg gcagctcagg accggcttcg
     1861 tgaggcgttc cggcggcgcg attcacggga cgcgaccacc ctagctgagc acctcgcgga
     1921 cgccaaaatt ggcattcccg gcacttctac ccctttcctc cggcgcccgt ttgaagccgc
     1981 agcttcgcgc tgcaaccggt tgattctgat aagaaattat tgagtaacgg caaagtttat
     2041 caattaagac tttgatcatg aaggttttgc ataattttta gacggcgcag ttccgatccc
     2101 ggcgggagag ccagtgcgcc tgaaaccgca ggacctcaca ggcagtctct attattatgg
     2161 ctattgcatc ccgattctcg ctcgcggcaa ggctctattc gatcttcata ctattcgcga
     2221 tcctgaccgc agccatcacc gtcttgagcg actacaacag ccgtcaaggc gccgagctca
     2281 ccgacgccat cgagaaggcc aatatggccg cactcaatgt cgagcgcgtc aactcgctgg
     2341 tctacgcggt ggtgatggaa tcccgcggca tctacatgtc caccgagcct gaggtggtaa
     2401 aaaaatatgg cgaaggtctg ctcaagttca ctggcaacat cctcgccgtc gtgaatcgat
     2461 gggagtccat cgttcaggct gacgacgccg agcagttcgc tgcattcagg aagcgcatcg
     2521 agcagttcgt cgacttccgc aaagagcttg tgcgtcgtgc caacgagatc agcccggcgg
     2581 ccggccgcga atggggtgac aacgatgcca accgctccgt gcgctccgcg ctcaacaagg
     2641 atctcgagac gctgtcgaag gtctatgtcg aacgcggcaa gcaaatcgcc gaacgggcca
     2701 aactcaaccg ccagctttcc tttgtgctga cctgtctcgg cggcgtcgcg ctgctgttgg
     2761 tcgtgttcgg cgtcgccatc atcggccgct cgatcgcccg tccgctctcg gccatcacct
     2821 cgaccatcaa gcaagtcgcc gacggcgccg aaaatgtcgt agtgccccat accgatcgcg
     2881 ccgacgagat cggggcgctg gcccgcgcca tccggatctt ccaggaggcg atggagcgca
     2941 accgcaagct caattcgcag gtttcggcgg attcgaaagc ccgcgaagag cgtagtcggc
     3001 acatcgaggc ctccgtcgaa gcgttccggc aggcgatcgg cgccgtcctg cgcgccgtca
     3061 acgacaatgc gctggccatg cgcaacgccg cgcagaccat ctcccgcgtc acgtccgacg
     3121 ccaacgagcg cgcggtcgca gccgccggcg cgaccacaca ggcctccggc aacgtctcgg
     3181 cggtcgcggg cgccacggag gagctctcga cgtcggtgca ggaaatcggg cgacaggtcc
     3241 ggcaatccgc cggtgcggtc gagcagaccg gccagcgcac cgaaaagtcg atcgccgaaa
     3301 tcgagagcct cgctgccgcg acgcagcgga tcgacggcgt gctgcaattg atccaggcga
     3361 tcgccgagca gaccaatctt ttggcgctga acgccaccat cgaagccgcg cgcgccggcg
     3421 atgcg
//
LOCUS       DATLJT010000762         2904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_48230,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000762 DATLJT010000000
VERSION     DATLJT010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48230"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VM910_17360"
     CDS             <1..433
                     /locus_tag="VM910_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HVI64330.1"
                     /translation="ESEKAKTPFRRLMIAQDTGSAITGPARADLYFGSGADAGKVAGR
                     LKQNVRFVMLVPKSLDPEERARKMPLPDPRPSEKIAKLLPQNQLAKDKKDTSPAQTSA
                     VAQAQTGTPVVAPVPLPEARPATAPSREPLRRGRLRQRSTQ"
     gene            430..1020
                     /locus_tag="VM910_17365"
     CDS             430..1020
                     /locus_tag="VM910_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HVI64331.1"
                     /translation="MKRPPSLPIPDSASSRGRRGLTEEERALWESVAKQIKPLRKKTR
                     AARQTAVPALEPSSEKAAATPRPIAQVHAPKPSKPAAPPLAPLGRRERSRLSRDRDAI
                     DARIDLHGMTQTRAHRALFGFLQRAHRDGLTFVLVITGKGKVGGSEDERGVLRRQVPQ
                     WLSLPEFRSLVVGFEEAHIGHGGEGALYVRVRRARF"
     gene            complement(1045..2346)
                     /gene="hslU"
                     /locus_tag="VM910_17370"
     CDS             complement(1045..2346)
                     /gene="hslU"
                     /locus_tag="VM910_17370"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HVI64332.1"
                     /translation="MTDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDGGL
                     REEVLPKNILMIGPTGVGKTEIARRLARLAGAPFIKVEATKFTEVGYVGRDVEQIVRD
                     LVEVAIAQVRERKRKDVQARAQLAAEERVLDALVGPNSSAATRDSFRRKLRAGELNDK
                     EIEIETQSSGGFPMFEIPGMPGAQMGAISIGDIFGKLGGRTKTRRLTVEGSHEILVNE
                     ESDKLLDNEQLVQEAIGAVENNGIVFLDEIDKICVRDNRIGGDVSREGVQRDLLPLIE
                     GTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRVELQALTRDDMRRIL
                     TEPEASLIKQYVALMKTEGVTLDITDGAIDALADIAVAVNSSVENIGARRLQTVMERV
                     LDEISFTAPDRNGETVQVDADYVQKHVGDLARNADLSRFIL"
     gene            complement(2397..>2904)
                     /locus_tag="VM910_17375"
     CDS             complement(2397..>2904)
                     /locus_tag="VM910_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HVI64333.1"
                     /translation="LFAMGRLPICACGYVKLWHGVARSSENSQHLTDWYTFSHLIHGF
                     LFYAATWVLLPRLSWQARLIVAMLVEGGWELVENSNFVIERYRAATVSLDYFGDSIVN
                     SISDTLAMVTGFLLARTLPVAMTVALALAFEIFVGLHIRDNLTLNIIMLLYPFEAIRQ
                     WQAGPPII"
BASE COUNT          540 a          917 c          952 g          495 t
ORIGIN      
        1 cgaatcggag aaggcgaaga cgccattccg ccggctgatg atcgcgcagg ataccggctc
       61 ggcgatcacg gggccggcgc gcgccgacct ttatttcggc agcggagcgg atgcgggcaa
      121 agtcgccggc cgactcaagc aaaatgtgcg ctttgtgatg ctggtgccca aaagcctcga
      181 tccggaagag cgagcccgaa agatgccgtt gccggatccg cggccgtcgg agaagatcgc
      241 aaagctgtta ccgcagaacc agcttgcaaa ggacaagaag gacacctcgc ctgcacagac
      301 ttcggccgtg gcgcaagccc agaccggcac gcccgtcgtc gcgcccgttc cgttgccgga
      361 ggcgcgcccc gccactgcgc cgagtcgcga gccgctgcgt cgcggtcgtc tgcgccagcg
      421 cagcacgcaa tgaaacgtcc gccttccctt ccgattcccg actccgcttc gtctcggggc
      481 cggcgcggcc tgaccgagga ggagcgcgcg ctctgggaaa gcgttgccaa gcagatcaag
      541 ccgctccgca agaagacgcg tgcagcgagg caaacggccg tgccggcgct ggaaccgtcg
      601 agcgagaagg cggccgccac gccgcggccg atcgcacagg tgcatgcgcc aaagccgagc
      661 aagcccgccg cgccgccatt ggcgccgctc ggacgccgcg agcgttcgcg gttatctcgc
      721 gaccgggacg cgatcgatgc gaggatcgat ctgcacggca tgacacagac gcgcgcccat
      781 cgggcgttgt tcggcttcct gcagcgtgcg catcgcgacg gcctgacctt cgtgctggtc
      841 atcaccggca agggcaaagt gggcggctcg gaagacgaac gcggcgtgct gcgccgccag
      901 gtgccgcaat ggctgagcct gccggagttc cgttcgctgg tcgtcggctt cgaggaagcc
      961 catatcggcc atggcggcga gggcgcgctt tacgtgcggg tccggcgcgc gagattttag
     1021 cgcgcgtctt gtctgcggaa tccgttacag gatgaagcgg ctgagatccg cgttcctggc
     1081 gagatcgccg acatgcttct gcacataatc tgcgtcgacc tggaccgtct cgccgttgcg
     1141 gtcgggcgcg gtgaaggaga tttcgtcgag cacgcgctcc atcacggttt gtaggcgccg
     1201 cgcgccgatg ttttcgaccg aggaattgac ggcgaccgca atgtcggcga gcgcgtcgat
     1261 ggcgccgtcg gtgatatcaa gcgtcacgcc ctcggtcttc atcagcgcca catattgctt
     1321 gatcagcgac gcctcgggct cggtcaggat gcggcgcatg tcgtcgcggg tcagcgcctg
     1381 caactcgacc cggatcggca gtcgcccttg caattccggc aacaggtcgg aaggctttgc
     1441 gatgtggaag gcgccggagg caatgaacag gatatggtcg gtcttgaccg caccgtgctt
     1501 ggtggagacc gtggtgccct cgatcaacgg cagaagatcg cgctgcacgc cttcgcgcga
     1561 gacgtcgccg ccgatacggt tgtcgcgcac gcagatcttg tcgatctcgt cgaggaatac
     1621 gatgccgttg ttctcgacgg cgccgatcgc ttcctgcacg agctgttcgt tgtcgagcag
     1681 cttgtcggat tcctcgttga ccagaatctc gtgcgagcct tcgacggtga gacggcgggt
     1741 cttggtgcgc ccgccgagct tgccgaaaat gtcgccgatc gaaatcgcgc ccatctgcgc
     1801 gcccggcatg cccggaattt cgaacatcgg gaaaccgccg gacgattgcg tctcgatctc
     1861 gatctccttg tcgttgagct cgccggcacg cagcttgcgg cggaaggagt cgcgcgtcgc
     1921 ggcgctggaa ttgggtccga ccagcgcgtc gagcacgcgc tcctcggcag ccagttgcgc
     1981 acgcgcctgc acgtccttgc gcttgcgctc gcgcacctgc gcgatcgcga cctcgaccag
     2041 atcacgaacg atctgttcga cgtcgcggcc gacatacccg acctcggtga atttcgtcgc
     2101 ctcgaccttg atgaacggcg cgccagcaag ccttgcgagc cgccgcgcga tctcggtctt
     2161 gccaacgccg gtcggcccga tcatcaggat gttcttcggc agcacctcct cgcgcaggcc
     2221 gccatcgagc tgcagccggc gccagcgatt gcgcagcgcg atcgagaccg cgcgcttggc
     2281 gtcggcctgg ccgacgatga aacgatcgag ttcggagacg atttcacggg gggagaagtc
     2341 tgtcatgggc tttagctagg actggaatgt cgcgccgaca aggcattttc gaaagttcag
     2401 ataatgggcg gccccgcctg ccattgccgg atcgcctcga aagggtaaag cagcatgatg
     2461 atattgagcg tcagattgtc gcgaatgtga agccccacga aaatctcgaa agccagcgcc
     2521 aaggccacgg tcatggcgac aggaagcgtt ctggcaagca gaaagcccgt caccatggcg
     2581 agcgtgtcgg aaatcgaatt gacgatgctg tcgccgaaat aatccagcga gacggtcgca
     2641 gcgcggtagc gctcgatgac gaagttggag ttttcaacga gctcccagcc gccctcgacg
     2701 agcatcgcga caatcaggcg agcctgccag gacaggcgtg gcagcaagac ccaggtcgca
     2761 gcatagaaca ggaaaccgtg gatcaggtgc gagaacgtat accagtcggt gagatgttgc
     2821 gagttctccg aactccgcgc gaccccgtgc cacagtttga catagccgca agcgcagatc
     2881 ggcaggcgac ccatcgcgaa aagg
//
LOCUS       DATLJT010000763         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2345253,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000763 DATLJT010000000
VERSION     DATLJT010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345253"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..808)
                     /locus_tag="VM910_17380"
     CDS             complement(41..808)
                     /locus_tag="VM910_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI64334.1"
                     /translation="MRILLPVIVAAAGTAIWELLVRIFDIQPYVLPGPFAVLRTLFSD
                     WGVLSQSLLVTLLTTLEGFVAASVGGIALALLFNLSKVLEYSLFPYAVILQVTPVIAI
                     APLLLIYLPQQTAVVVCAWIVGFFPVLSNTTLGLNSVDRNLAGLFQLYGATRLQTLRF
                     LKLPSALPYILGGLRIAGGLSLIGAVVAEIAAGTAGAGSGLAYRIAESGYRLNIPRMF
                     AALLLLSLAGIVIYALLALISHLALRRWHESALGKET"
     gene            complement(874..1677)
                     /locus_tag="VM910_17385"
     CDS             complement(874..1677)
                     /locus_tag="VM910_17385"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64335.1"
                     /translation="MSDTDIDIAVAGSAVRLGGVTKAYDTGVVALGPFDLSVRKGEFV
                     SLLGPSGCGKSTALRLIAGLVQPSSGRVEVIKHAGDARPGHAIGFVFQEPTLMPWTSV
                     RENVRLPLRLARIPVADADARIDEALTRVGLAEFSDSYPRELSGGMKMRVSLARALVT
                     DPDILLMDEPFAALDEITRFRLNNDLLALWRNLKKTVIFVTHSVFESVYLSQRVVVMT
                     QRPGRLSAEIRIETAEPRGEDFRTSAAYAEYCREVSAALAPSYEGRSAL"
     gene            complement(1693..>2127)
                     /locus_tag="VM910_17390"
     CDS             complement(1693..>2127)
                     /locus_tag="VM910_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64336.1"
                     /translation="GFKPNVLLLADYGFNTYSTLIETRADMIIKKPDLVQRFVDASIV
                     GWYNYLYGDNSAANAMIKKLNPEMTDELLAYSIAKMKEYGIVDSGDAPKNGIGAMTDE
                     RVTSFFDKMVRAGVVKPDLDYRKSYTLRFVNKGVGLELRPKK"
BASE COUNT          443 a          658 c          682 g          344 t
ORIGIN      
        1 ccatggacaa ccagatcgat cttttcggaa gaaacggcca tcaggtttcc tttccaagcg
       61 cgctttcatg ccaacgccga agtgcgagat gggaaattag cgccagcagg gcgtaaatga
      121 caatcccggc gagcgagagc aaaagcaatg ccgcaaacat gcgaggaata ttgaggcggt
      181 agccggattc ggcgattctg taggcaaggc cggaaccggc gcccgcggtg ccggcggcga
      241 tttcggcaac gacggcgcca atcagcgaca gtccgccggc aatgcgcaag ccgccgagaa
      301 tatagggcaa ggccgatggc aatttgagga atcgcagcgt ttgcaggcgc gtggcgccat
      361 agagctgaaa caggccggcg agattgcggt ccaccgagtt cagtccgagc gtggtgttgg
      421 agagcacggg gaaaaaaccc acgatccagg cgcagacgac caccgcggtc tgctgcggca
      481 ggtagatcag gagcagcggc gcgatcgcga tgacaggcgt cacctgcagg atcaccgcat
      541 aggggaacag cgaatattcc agcaccttcg acaggttgaa caacagcgcg agtgcaatgc
      601 cgccgacgct tgccgccaca aaaccttcga gcgtggtcaa cagcgtcacc aggagcgact
      661 gcgacagcac accccaatcg ctgaacagcg tacgcagcac ggcaaacggg ccgggcagca
      721 catagggctg gatgtcgaag atgcggacca gcaattccca gatcgcggta cccgcggcgg
      781 cgacgatgac gggaagcagg atgcgcatga cccgcagcgc acgattgtcc gccggctggc
      841 tggccgcggg cgcgcggtca ttctgcaccg tgctcacagt gccgacctcc cttcatagga
      901 gggcgcgagc gcggccgaaa cctcgcggca gtattcggca taggcggccg aggtcctgaa
      961 gtcctcgccc cgcggctcgg cggtctcgat cctgatctcg gcgctgagac ggcccggcct
     1021 ttgcgtcatc accaccacgc gctgcgagag atagacggat tcgaacaccg aatgggtgac
     1081 gaagatgacg gtcttcttca gattgcgcca cagcgcgagc aggtcgttgt tgaggcggaa
     1141 acgggtaatc tcgtcgagcg ccgcgaaagg ctcgtccatc aggaggatgt cggggtcggt
     1201 gaccagcgcc cgcgccagcg acacccgcat cttcatgcct cccgacaatt cccgggggta
     1261 gctgtcggaa aattcggcaa gcccgacgcg ggtcagcgcc tcgtcgatac gcgcatccgc
     1321 atcggctaca gggatccgcg caagcctcag gggcagccgc acattctcgc gcacgctggt
     1381 ccacggcatc agcgtcggct cctgaaacac gaagccgatc gcgtggcccg gccgcgcgtc
     1441 gccggcatgt ttgatcacct cgacgcggcc ggaggacggc tgcacaagtc ccgcaatcag
     1501 ccgcagcgcg gtcgacttgc cgcaaccgga cggcccgagc agcgagacga actcgccctt
     1561 gcggacggaa agatcgaacg gcccgagcgc cacgacgccg gtgtcatagg ccttggtgac
     1621 gccgccaagc cgcaccgcgg atccggcaac ggcgatgtcg atgtcggtgt cggacaaatc
     1681 cgcctcggcc acttattttt tcggccgcag ttcgaggccg acgcccttgt tgacgaagcg
     1741 aagggtgtag gacttgcggt aatcgaggtc gggcttgacc acgccggcgc gcaccatctt
     1801 gtcgaagaag ctcgtcaccc gctcgtccgt catggcgcca atgccgttct tcggcgcgtc
     1861 gccggaatcg acgatgccat attccttcat cttggcaatc gaataggcga gtagctcgtc
     1921 ggtcatctcc ggattgagct tcttgatcat cgcattcgcc gccgaattgt cgccgtagag
     1981 atagttgtac cagccgacga tcgacgcatc gacgaagcgc tgcaccaggt ccggcttctt
     2041 gatgatcatg tcggcgcgcg tctcgatcag ggtggagtag gtgttgaagc cgtaatcggc
     2101 gagcagcagc acgttcggct tgaagcc
//
LOCUS       DATLJT010000764         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1656787,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000764 DATLJT010000000
VERSION     DATLJT010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656787"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VM910_17395"
     CDS             <1..389
                     /locus_tag="VM910_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64337.1"
                     /translation="LKSSPPKAALRFESVPTGAEVKVSGQSCRTPCELTLDVAELSAT
                     FSHKGYQPQTIAVRSESESFFAVARFVPNPVHADLQRIGATAKRQQRKETPVAAENPS
                     GPVSAEATASSNAPANSAGWSYGDVK"
     gene            complement(972..1905)
                     /locus_tag="VM910_17400"
     CDS             complement(join(972..1560,1559..1905))
                     /locus_tag="VM910_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611771.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI64338.1"
                     /translation="MPKAYSADMRERVIAEVEGGASRREAAEEFEVSASTAIIWVKCF
                     RETGRCAAKPRGGSISALEKHADFLLGLIETQSDLTLDEVVLAMRKHKIPGGRTAVWR
                     FFQRHKITFKKKPARAAEQERADVARARRRWMREQGMFDPAHLVFVDETAANTKMVRL
                     SGRCRRGERLIGRVPQGHWKTITFVAALRRNGMRAPCTVDGSMNGAKFLAYVKQCLAP
                     TLKRKDNVVIDNLPAHKAAGIREAIEARGATLRYLPQYSPDLNPIEMPFSKLKACLRK
                     AAERTIPRLRRRIGRFACTLTAREARNYFRHAGYE"
     gene            complement(1927..>2452)
                     /locus_tag="VM910_17405"
     CDS             complement(1927..>2452)
                     /locus_tag="VM910_17405"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64339.1"
                     /translation="RPPAGLIEAFRQGLREFGYVEGENIALEMRIAEGDNQELEALAR
                     ELVELKVDVILAVNTPAVQAAKKATGSLPIIMTRLADPVKTGLVSNLSRPGGNITGLS
                     FMSDELSGKRLALLKEALPKISRVAALWYDGNPGATIAVDGMKAPSRELEHVPPQLNR
                     GDSREAKDRRVYRH"
BASE COUNT          510 a          746 c          689 g          507 t
ORIGIN      
        1 ttctcaagtc atcgccgccg aaagcggcac tacgcttcga atccgtacca acgggcgccg
       61 aggttaaggt ttcagggcag agctgtcgca cgccgtgtga gctcacgctc gatgtggcgg
      121 aactttcggc gacgttctca cacaagggct atcagccgca aactatcgcg gtgcgttccg
      181 aatcggaatc gttctttgca gttgctcggt ttgtccccaa cccggtgcat gcagatcttc
      241 agcggattgg ggcgacggcc aagaggcagc aaaggaagga aacaccagtc gcggccgaaa
      301 atccttccgg acctgtttcg gcggaggcaa cggcctcctc gaatgcgcca gccaacagcg
      361 cgggctggtc ttatggcgat gtcaaataaa atcgacgcgt gaccaagcag ggtggcatca
      421 accaccgtca gactccaatc aaactaagcg gcaaggcccg ggctccagcg gtctcatcct
      481 agccaactca gattttcgtt tttcgcccgg caataatgaa gcgcgattcg cgcactccta
      541 gaaggagtag ccaacgagga ggcatagcac tggttacaag ctcgcgaacg tcggccatga
      601 tactcggtcg ctggcgcatt acctcgggca taggaacctg caatcgatgg cgaggtatac
      661 ggcgctggcg cctggaaggt ttgcggggtt ctggcgggac taaaacagga ggagcaccga
      721 cccgtatcgg tgctccttct aaccaccggt atcgaacggg acggatacca gttacctcac
      781 cagaatagtg caagatcagc gagccgtcac caaccgagta gcgcttctgg ttagtctgaa
      841 gcgatgggcg gagggcctga gagcaaacca tcggagggac gatcagttta ctccccagtc
      901 tccgtcgaac tgctgctttg cgtgaggggc tgaactctcc gggtggcggc tagagcagat
      961 tccgttcgaa ttcactcata gcccgcgtgc ctgaaatagt tgcgagcttc gcgggcggtt
     1021 aacgtgcagg cgaacctgcc aattcggcgg cgcagacgag gaattgttcg ttccgctgcc
     1081 ttgcgcaaac acgccttcag tttgctgaac ggcatctcga tggggttcag gtcgggcgag
     1141 tattggggca gatagcgaag cgtagcgccg cgcgcttcga tcgcctcacg aatgccagca
     1201 gctttgtgcg caggaagatt gtcgatcacg acgttatcct tgcgcttgag ggttggagcc
     1261 agacactgtt tgacataggc cagaaacttg gctccgttca ttgagccatc aacggtgcac
     1321 ggcgctcgca tgccgttgcg acggagcgcg gccacaaagg tgatggtctt ccaatgcccc
     1381 tgcggcacac ggccgatcag gcgctcgccg cgccggcaac ggccgctcag ccgcaccatc
     1441 ttggtattgg cggcggtctc atcgacgaac acaaggtgcg caggatcaaa catgccctgt
     1501 tctcgcatcc agcgccggcg tgcccgcgcc acatccgcgc gctcttgctc cgccgcgcgc
     1561 aggctttttt ttgaaagtaa tcttgtgacg ctgaaagaag cgccagactg ccgtgcggcc
     1621 gccaggaatc ttgtgcttgc gcattgccaa aaccacttcg tccagtgtca ggtcagactg
     1681 cgtctcgatc aggcccagca gaaaatccgc atgcttctcc aaagccgaga tgcttccgcc
     1741 ccgcggtttg gccgcgcagc ggcccgtctc tcgaaagcac ttcacccaga taatcgccgt
     1801 actggcactg acctcaaact cctcggctgc ctcgcgccgc gaagctccgc cttccacttc
     1861 cgcaatcacg cgctcacgca tgtccgcaga ataggccttc ggcattgcca gcctcccaaa
     1921 agcgaatcaa tgccgataga ctctcctatc cttcgcctca cgagaatccc cccgattcaa
     1981 ttggggcgga acatgctcta gctctcggct cggagctttc atgccatcca cggcgatagt
     2041 tgctcctgga ttgccgtcgt accagagagc agccactcgg ctaatcttgg ggagagcctc
     2101 tttcagcagc gctagtcgct ttccacttaa ctcatcgctc atgaagctca aaccggtaat
     2161 attcccaccc ggtcgggata ggttagacac gagaccggtc ttcaccggat cagcaagcct
     2221 agtcatgatt attgggaggc tgccggtcgc tttcttggcg gcctgtaccg ctggcgtatt
     2281 gacggcgaga atgacatcga ctttgagttc cactaactcg cgagcgaggg cttcaagttc
     2341 ttggttgtct ccctccgcga tacgcatctc caacgcgatg ttttctccct cgacgtatcc
     2401 gaactcacgc aggccttgtc gaaaagcctc aatcaatcct gcaggtgggc gt
//
LOCUS       DATLJT010000765         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2345330,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000765 DATLJT010000000
VERSION     DATLJT010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345330"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VM910_17410"
     CDS             complement(<1..1192)
                     /locus_tag="VM910_17410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI64340.1"
                     /translation="MTSFHDFGLAEPITRALAEEKYATPTPIQAQTIPAVMSRRDVIG
                     IAQTGTGKTAAFAVPILHHLFTNRRRPARKTCRVLVLSPTRELSGQILDSFRAYGRHV
                     GTSVALVIGGVSMGAQVRAVQSGVDVLVATPGRLLDLVTSNALRLGEVEFLVLDEADR
                     MLDMGFIHDIRKIVAKLPAQRQTLLFSATMPRAIATLADQMLRDPARVAVTPVASTVD
                     RVEQRIIRVDRPAKPAVLAEILQRESIGRVLVFTRTKHGADKVVRGLAKAGIPAHAIH
                     GNKSQNQRERVLAAFRKGEVLTLVATDIAARGIDVDGISHVVNFDLPNVPETYVHRIG
                     RTARAGAAGVAISLCDSEEAAYLRDIEKLIRMSIPATDHRTFPRQARPSTARGGTSRP
                     SAHAA"
     gene            1623..1865
                     /locus_tag="VM910_17415"
     CDS             1623..1865
                     /locus_tag="VM910_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64341.1"
                     /translation="MAPITGVTEEQLTRALGEAVVRTWSHLPKDVQNCLFQEAVTSQG
                     ESIRPQLAVFLHDKHPRTLDPLGKPREMMEPDSLGG"
     gene            complement(1916..>2360)
                     /locus_tag="VM910_17420"
     CDS             complement(1916..>2360)
                     /locus_tag="VM910_17420"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI64342.1"
                     /translation="DLVNEHFRLLQKIIASERGAVVKTIGDAVMATFETPDRAIAAAI
                     RMREAMSDLGAERQHQSLHLKMGIHEGSCLAVTLNDQQDYFGQTVNIASRVQGLAASR
                     SIVVTEPVVENAQASALLENNGLKPTLRRVALSGIAGEMSVYEIP"
BASE COUNT          418 a          720 c          786 g          436 t
ORIGIN      
        1 cggcggcatg cgccgagggc cgcgatgtgc cgcctcgggc cgtcgaaggc cgggcctgtc
       61 gcggaaacgt tcggtggtcc gtcgccggaa tggacatgcg gatcagcttc tcgatgtcgc
      121 gcaggtacgc cgcctcttcg ctgtcgcaga gcgagatcgc gacgcctgct gccccggctc
      181 gcgcggtgcg gccgatgcgg tgtacatagg tttccgggac gttcggcaga tcgaaattca
      241 cgacgtggct gatgccgtcg acatcgatac cgcgcgctgc aatgtcggtg gcgaccagcg
      301 tgagcacttc ccccttgcga aatgcggcga gcacgcgttc gcgctggttc tgcgacttgt
      361 tgccgtggat ggcgtgggct gggatgccgg ccttggcgag gccgcgcacc actttgtcgg
      421 cgccgtgctt ggtgcgggtg aacacaagca cgcggccgat cgactcgcgc tgcaaaattt
      481 cggcgaggac cgccggtttc gccggccgat cgacgcggat gatgcgctgc tccacgcggt
      541 cgacggtgga ggctaccggc gttaccgcaa ccctggccgg gtcgcgcaac atctgatccg
      601 cgagcgtggc gatcgcgcgc ggcatcgttg cggaaaacaa cagcgtttgc cgttgcgcag
      661 gaagcttcgc gacgattttg cgaatgtcat ggatgaagcc catatccagc atccggtcgg
      721 cttcatcgag caccaggaat tccacttcgc cgagacgcag cgcgttgctt gtcacgaggt
      781 cgagcagacg cccgggcgtg gcgacgagca cgtcgacgcc gctttgcacc gcgcgcacct
      841 gcgcgcccat cgacacgccg ccgatcacca gcgcaacact ggtgccgacg tggcggccgt
      901 aagcgcggaa actatcgagg atttggccgg acagctcgcg tgtcgggctc agcaccagta
      961 cacggcaggt cttgcgcgcc gggcgccgtc tattcgtgaa cagatgatgc aggattggca
     1021 ccgcgaaggc cgcggttttg ccggtaccgg tttgcgcaat cccgatcacg tcgcggcgcg
     1081 acatgacggc gggaatggtt tgcgcctgaa tcggcgtggg cgtggcgtat ttttcttccg
     1141 cgagcgcgcg cgtgatcggt tcggcaaggc cgaagtcatg aaaagaggtc aatgaacaag
     1201 ctttctatgg acgagacggc gcgccgcagc gacctacgct gcagccccat atcgcggatg
     1261 gtgcaagaca cccccgcgtg atctgggctg tcggtgaaag tgaaggggca agccagaaac
     1321 cgtttgacgg ggatccgtac acgcggctcg ccaggaccag atgaggtcgc gcagggcata
     1381 tggggcatag atgtgttggt ttcaaggcgc cccggattta acccggggct gtcgcatcag
     1441 ccggtgtggt tactaccgag gctattgtgt gccttgctgc acataaagcg cgagcatggc
     1501 cgccgccacg caaggatggc acacgcacgg ccctgtcggc gtaagatgct gggccgcggt
     1561 accggaactc cacgtcctat cggacgttgg cgtgtcgtct ttttcctgcg ggagtggcaa
     1621 ccatggcccc aatcaccggc gtgactgagg agcagcttac acgtgcccta ggcgaggccg
     1681 ttgtccgaac atggagccat ttgcctaaag acgtgcaaaa ttgcctcttt caggaggcgg
     1741 tgacgtccca gggcgaatcc atcagaccac agcttgcggt cttcctgcac gacaagcatc
     1801 cgcgcacatt ggacccatta ggcaagccgc gcgaaatgat ggagccggac agcctgggcg
     1861 gttagcggag gtccactacc tcgttgatgc ggctgccgaa gcggcgcgcc gatgctcaag
     1921 ggatctcgta gaccgacatt tcgccagcga tgccgctcag cgcgacgcgc ctgagcgtcg
     1981 gctttagccc attgttctcc agcagggcag aggcttgcgc gttctcgacc accggttccg
     2041 tcaccacgat cgagcgtgag gccgcgaggc cttggacgcg cgaagcgata ttgacggtct
     2101 ggccgaaata gtcctgctgg tcattaagcg tgaccgcgag gcacgatcct tcatggatgc
     2161 ccatcttcag atgcaggctc tggtgctggc gctccgcgcc gagatcgctc atcgcctcgc
     2221 gcatgcggat cgcggcggcg atggcgcggt caggggtctc gaaggtcgcc atcacggcat
     2281 cgccaatggt cttcacgacg gcaccccttt cggaggcgat gatcttctgc agcagcctga
     2341 aatgctcgtt gacgagatcg
//
LOCUS       DATLJT010000766         7785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3264540,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000766 DATLJT010000000
VERSION     DATLJT010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264540"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VM910_17425"
     CDS             complement(<1..183)
                     /locus_tag="VM910_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64343.1"
                     /translation="MTITATAGGNVRTGGMTAEERKVIFASSLGTVFEWYDFYIYGTL
                     GAFLAKYFFSNVPPAVG"
     gene            530..1204
                     /locus_tag="VM910_17430"
     CDS             530..1204
                     /locus_tag="VM910_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI64344.1"
                     /translation="MPAANSHLVIADDHPLFRDALRQAVGSVVASAKIDEAGSFEDLT
                     TLLEEDSDVDLILLDLTMPGISGFSGLIYLRAQYPAIPVVIVSASDDGVTIRRSLDFG
                     ASGFIPKRFGVETLRDAIVKVMDGDIWVPPDTDLSSAVDPDMTRLRDRLVTLTPQQVR
                     VLMMLSEGLLNKQIAYELGVSEATIKAHVSAILQKLGVESRTQAVIAAAKIAGSQWRQ
                     PTPSEP"
     gene            complement(1208..4714)
                     /locus_tag="VM910_17435"
     CDS             complement(1208..4714)
                     /locus_tag="VM910_17435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HVI64345.1"
                     /translation="MLHDWSVIAAAFGYISFLFFVASYGDRLSPAQRGRASTWIYPLS
                     LAIYCTSWTFFGSVGFATRTSVDFLAIYLGPVVLIGLCTPLLRRVIRLAKGQNITSIA
                     DFIGARYGKSQAVAATVAMIAIIGSVPYIALQLKAVASSLETILSEDQAISKIPLLGD
                     IALMVTLTMAIFAVLFGTRQTDATEHQHGLMLAVATESIVKLVAFLAAGAFVTFWMFE
                     PQELIQRAMKTPEAVRAIEYVPSIGNFLCMTALSFCAIMLLPRQFHVSVVENSTDAEV
                     SRARWLFPLYLVAINLFVIPIALAGLITFPFGAVNSDMYVLALPIEGHAELLSIGVFV
                     GGLSAATAMVIVECVALSIMVSNDIVVPLVLQRAPESRTHGKDFGDFLLKVRRVSIFA
                     IMVMAYFYYRALGNTQLAAIGLLSFAAIAQLAPVFFGGLLWRRATARGAIGGMLVGFA
                     VWTYTLFVPSFLDTSTAGLLFLQHGPFGIEALRPQALFGADLPPLLHGVLWSLGLNLL
                     TYVVLSLAHQPSSIERLQADLFVPDALAPTTPTFRRWRTTITVQDIQSTVAQYLGPER
                     AQLSFEAFANTHHTTLDPAAPADFELLQHAEHLIASSIGAASSRLVMSLLLRKRTVSA
                     KAALKLLDDSHAALHFNREILQTALNHVRQGIAVFDVDLQLICSNRQFGELLQLPSHL
                     VQIGIPLQEILEFMGTNTPSAAGEAASMERRLAAYTTEGEPYLERLPDRHMVIEVRSN
                     RMPDGGLVITFTDVTPSFEAAEALERANATLEKRVRDRTEELTRLNSELALAKSSAED
                     ANISKTRFLAAASHDILQPLNAARLYVTSLVERQNGGDDSRLVENIDDSLEAIEEIIS
                     ALLDISRLDAGAMTPSITSFKMADLMRSLEIEFVPIARAKGLTLTFVPCSLPVESDRL
                     LLRRLLQNLISNAIKYTPQGRVLVGCRRHGQSLQVCVYDTGVGIPVLKRGEIFKEFHR
                     LEQGARIARGLGLGLSIVERLARVLHHGIALDSNPGGGSMFSVTVPTAKGINYTAAVT
                     SATPLSRTPISGARIVCIENDPAILDGMKTLLTAWDAEVIAVADPDAATEAIEAAGGR
                     VTGLLVDYHLDRGNGVAAIRDIRRRFGENIPAILITADRSPHVRAAAREENIAVLNKP
                     LKPASLRALLGQWRTQQMVAAE"
     gene            complement(4900..6129)
                     /gene="hemA"
                     /locus_tag="VM910_17440"
     CDS             complement(4900..6129)
                     /gene="hemA"
                     /locus_tag="VM910_17440"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HVI64346.1"
                     /translation="MDYSEFFNAALGQLHDERRYRVFADLERIAGRFPHAVWHSPKGP
                     RNVVIWCSNDYLGMGQHPKVVGAMVETATRVGTGAGGTRNIAGTNHPLVQLEAELADL
                     HGKEAALLFTSGYVSNQTGIATIAKLIPNCLILSDALNHNSMIEGVRQAGRERQIFRH
                     SDMAHLEELLRAAGPDRPKLIVCESLYSMDGDIAPLARICDLAERYGAMTYVDEVHAV
                     GMYGPRGGGIAERDGVMHRIDVLEGTLAKAFGCLGGYIAAKTEIIDAVRSYAPGFIFT
                     TALPPPICSAATAAIRHLKASNWERERHQDRAARVKAILSAAGLPVMSSDTHIVPLFV
                     GDPENCKRASDMLLEEHGIYIQPINYPTVAKGTERLRITPSPYHDDGLIDHLAEALLQ
                     VWERLSLPLKEQSLAAE"
     gene            complement(6299..7360)
                     /locus_tag="VM910_17445"
     CDS             complement(6299..7360)
                     /locus_tag="VM910_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI64347.1"
                     /translation="MHSKSDKIQSSAEALRYPYENHPGPDQVVELMPGVLWVRLKLPF
                     RLNHVNIYLLKDSDGWTAVDSGFGNEETIAAWTSLIEGPLKDIRITRLIVTHSHPDHV
                     GLAGWIVERFGCPLVMSQVEYLQSVYHQNRGTEERRNAQRLFFRRHGMDESLTDKLLG
                     RGQDYLKRVSILPPGYRRISHGDEITIGTRRFKVITGGGHALDQVMLYCAADKLFLSA
                     DQVLSKISPNVSVWAVEPDQNSLGEYLASLASLTTTLPYDVLVLPGHGVPFYGLKTRI
                     KQLADHHEERCRLIADACRDVPQTSKELVPVVFHKHALDAHQMGFAAGELIAHVNYML
                     VEGRLTVQETDGVLRFRTT"
     gene            complement(7438..>7785)
                     /locus_tag="VM910_17450"
     CDS             complement(7438..>7785)
                     /locus_tag="VM910_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64348.1"
                     /translation="RDKHHGFLSGNAGITGVFLYAGTDCVGYAYIDSGGHIGPLAVAE
                     PGLMSAAFATAFKLAAETGTSEVSAFLPGINHAALDAATAHGMRITFPMVLMSAREFG
                     DFSRYLPRNPGFM"
BASE COUNT         1494 a         2358 c         2559 g         1374 t
ORIGIN      
        1 gccaaccgcc ggaggcacgt tggagaagaa gtactttgcc agaaacgcgc cgagcgtgcc
       61 gtagatatag aagtcgtacc actcgaacac ggtaccgagc gaagaagcga aaatgacctt
      121 tcgctcctca gccgtcattc ctcctgtgcg gacattgccg cccgctgtcg cagtgatcgt
      181 cattattcgc tccccgacaa taattgatgc tgggcctgcc ccctaaacgc gcgaaaactg
      241 gccgcattta tccaggggcc ttggcagcac ggtaacattg cggtgaaagc cgccccaata
      301 cgacttttgg ccctttacaa cgccaaatgg tgcacgcgga cccgtcggga tcaggcggct
      361 gccattccac ccatcttttg cggcgcacaa agcggcaaat tagcgcgacg ctatcctact
      421 gcattgcgcg cttgcatgcg aaatgccaac tattgtcgaa gaggaaaatt ctccgaaccg
      481 ccctttaggg gacgttgcgg tgtcatcgcg accgatgcaa aagacataaa tgcctgctgc
      541 caattcgcac cttgtgattg ccgacgatca tccgctgttc cgtgacgcgt tgcgacaggc
      601 ggttggaagt gtcgtggcct cggccaagat cgacgaggct ggatcttttg aggatctgac
      661 cacgctcctg gaagaggatt ccgacgtcga tctgatcctt ctcgatctga ccatgccggg
      721 catttcgggc ttctccggcc tgatttacct gcgtgcgcaa tatccggcga tcccggtggt
      781 gatcgtgtcg gccagcgacg acggcgtcac cattcgccgc tcgctggatt tcggcgcgtc
      841 cggattcatt ccgaaacgtt tcggggtcga gaccttgcgc gacgcgatcg tcaaggtgat
      901 ggatggcgac atctgggtgc cgccggatac ggatctgtcg tcggccgtcg accccgacat
      961 gacgcggttg cgcgaccggc tggtgacgct gaccccgcag caggtgcggg ttctgatgat
     1021 gctgtcggag ggtctgctga acaagcagat cgcctatgag cttggtgtct cggaagccac
     1081 catcaaggcc catgtgtcgg cgatcctgca gaagctcggc gtcgagagcc gcacgcaggc
     1141 ggtgatcgcg gcggcaaaga tcgccggctc ccaatggcga caacctacgc cgtcggaacc
     1201 atagcgacta ctccgccgcc accatctgct gggtgcgcca ctggccaagc agggcgcgca
     1261 gcgaggccgg cttgagcggc ttgttcagca ccgcaatgtt ttcttcccgc gctgcggcgc
     1321 ggacatgcgg gctgcggtcg gcggtgataa ggatcgcggg aatgttttcg ccgaagcggc
     1381 ggcgaatatc ccggatcgcg gcgacgccgt tgccgcgatc cagatgatag tcgacgagga
     1441 gccccgtcac ccgtccgccg gcggcctcga tcgcctccgt cgctgcatcg ggatcggcga
     1501 cggcgatcac ctctgcatcc caggcggtca acagcgtttt catgccgtcc aggatcgcgg
     1561 gatcattctc gatgcaaacg atccgcgcac ccgagatcgg cgtgcgcgac agtggggtgg
     1621 cgctggtcac cgccgcggta tagttgatgc ccttcgccgt cggcaccgtc acagaaaaca
     1681 tcgagccgcc gccgggattg gagtcgagcg cgatgccgtg atggagcacg cgcgccagtc
     1741 gctccacgat cgagaggcca agcccgaggc cgcgcgcgat ccgcgcgccc tgctcgagtc
     1801 gatggaattc cttgaatatc tcgccgcgtt tcaggacggg aatgccgacc cccgtatcgt
     1861 agacgcaaac ctgcagcgac tggccgtgac gccggcagcc gaccagcaca cggccctgcg
     1921 gggtgtattt gatcgcgttg gagatcaggt tctgcagcag ccgccgaagc agcaggcggt
     1981 cggattcgac cggcagcgag cacggcacga aggtgagcgt cagtcccttg gcgcgcgcga
     2041 tcggcacaaa ttcgatctcc agcgagcgca tcagatcggc catcttgaag ctcgtaatcg
     2101 acggcgtcat ggcacccgca tcgagccgcg aaatgtccag aagcgcgctg atgatctcct
     2161 cgatcgcttc gagtgaatcg tcgatgttct cgacgaggcg cgaatcgtcg ccgccgttct
     2221 gccgctcgac caggctcgtc acgtagaggc gcgcggcgtt gagcggttga agaatgtcgt
     2281 ggcttgccgc ggcgaggaac cgtgtcttgg aaatgttggc gtcctccgcc gagctcttcg
     2341 cgagcgcaag ttccgaattg aggcgggtga gctcctcggt gcggtcgcgc acgcgtttct
     2401 caagcgtcgc attggcgcgc tcgagcgcct cggccgcctc gaaggaaggc gttacgtcgg
     2461 tgaaggtgat caccaggccg ccgtccggca tccggttgga gcgcacctcg atcaccatgt
     2521 gacgatcagg cagccgctcc agatagggtt cgccctcggt ggtataggca gccagccgcc
     2581 gctccatcga tgccgcctcg ccggcggcgg acggggtgtt ggttcccatg aattcgagga
     2641 tttcctgcaa ggggatgccg atctgcacca gatgcgatgg caactgaagc aactcgccga
     2701 actgccggtt cgagcagatc aactgcagat cgacgtcgaa caccgcaatt ccctggcgga
     2761 catgatttag cgcggtctgc aggatttcac ggttgaaatg cagtgcggcg tgggagtcgt
     2821 cgagcagctt caaggcggcc ttcgcggaga ccgtacgctt gcgcagcagc agcgacatca
     2881 cgagccggga ggaggccgcc ccgatcgagg aggcgatcag atgctcggca tgctgcagca
     2941 gttcgaaatc ggcaggtgcg gcgggatcga gcgtggtgtg atgggtgttg gcgaaggcct
     3001 cgaaggaaag ctgcgcccgt tcgggaccga gatattgcgc gaccgtgctc tgaatgtcct
     3061 gcacggtgat ggtggtacgc cagcggcgga acgtcggcgt cgtcggcgcc agcgcatccg
     3121 gcacgaacag gtcggcctgc agccgctcga tcgaggacgg ctggtgcgcc agcgacaata
     3181 cgacgtaggt caggagattg agtccgagcg accatagcac gccgtgcagc agcggcggca
     3241 aatccgcgcc gaacagtgcc tgcggccgca gcgcctcgat gccgaagggg ccgtgctgca
     3301 ggaacagcag gccggcggtc gaggtgtcga ggaaggaggg gacgaacagg gtgtaggtcc
     3361 acaccgcaaa tccgaccagc atgccgccga tggcgccgcg tgcggtggcg cgccgccaca
     3421 acaatccgcc gaagaagacc ggcgcaagct gcgcgatcgc ggcaaaggac aacagcccga
     3481 tcgcggcaag ctgcgtattg ccgagtgcgc gatagtagaa ataggccatc accatgatgg
     3541 cgaagatcga cacccgccgc accttcagga ggaagtcgcc gaagtccttg ccatgcgtgc
     3601 gcgattcggg tgcgcgctgc agcaccagcg gcacgacgat atcgttcgag accatgatcg
     3661 acagcgccac gcattcgacg atcaccatcg ccgtcgccgc cgacagcccg ccgacgaaca
     3721 cgccgatcga caacagttcc gcatgtccct cgatcggcag cgccagcacg tacatgtcgc
     3781 tgttgaccgc cccgaacggg aaggtgatga ggccggcgag cgcgatcgga atcacgaaca
     3841 ggttgatggc gaccagatac agcgggaaca gccagcgtgc gcggctgacc tcggcatcgg
     3901 ttgagttctc gaccacgctg acgtggaact gccgcggcag cagcatgatc gcgcagaacg
     3961 acagcgccgt catgcagagg aaattgccga tcgagggcac atactcgatc gcgcgcacgg
     4021 cctcgggcgt cttcatcgcg cgctggatca attcctgcgg ctcgaacatc cagaacgtga
     4081 cgaaggcgcc cgcagcgagg aaagccacga gcttcacgat ggattcggtt gccaccgcga
     4141 gcatcaggcc gtgctgatgt tccgtcgcat cggtctggcg cgtgccgaac agcaccgcaa
     4201 agatggccat ggtcagcgtc accatcagag cgatgtcgcc gaggagcggg attttggaga
     4261 tcgcctggtc ctcgctcagg atggtttcga gcgaggaggc cacggccttg agctgcagtg
     4321 cgatataggg aaccgatccg atgatggcga tcatcgccac ggtcgccgcg accgcctggc
     4381 tcttgccgta acgcgcgccg atgaagtcgg cgatcgaggt gatgttctgg cccttggcga
     4441 gccggatgac ccggcgcagc agcggcgtgc agagcccgat cagcacgacc gggccgagat
     4501 agatcgcgag aaaatcgacg ctggtacggg tggcgaagcc aaccgagccg aagaacgtcc
     4561 aggaggtaca gtagattgca agcgatagcg gatagatcca cgtgctggcg cggccgcgct
     4621 gcgccggcga caggcggtcc ccatagctcg ccacgaagaa caggaaggag atgtagccga
     4681 aggcggctgc aatcacgctc cagtcgtgca gcatcgctgc ttgctccctg tgcccgcccg
     4741 ttcggtgaat ctaacgcgtc atgccgcgat gcgacagcaa tttccttgaa ataattcagg
     4801 aattgcgatc gtcgttaaat ctgacgcggc gcggaatatg tctccggcgg gccccgggaa
     4861 cggggatcgc gcgatgcaac aaggcctaag ccggcgggat cattcggctg ccagcgactg
     4921 ctccttgagc ggcagtgaaa gccgctccca gacctgcagc agcgcttcgg cgaggtggtc
     4981 gatcaatccg tcgtcgtgat agggcgaggg cgtaatgcgc agccgctcgg tgcccttggc
     5041 gaccgtcgga tagttaatcg gctggatgta aatgccgtgc tcttccagca gcatgtcgga
     5101 ggcgcgcttg caattctcgg ggtcgccgac gaacagcggc acgatgtggg tgtcgctcga
     5161 cattacggga aggccggctg cgctcaggat cgctttcacg cgcgcggcgc gatcctggtg
     5221 gcgctcgcgc tcccagttcg aggccttcag gtggcggatc gcagcggtcg cagccgagca
     5281 gatcggcggc ggcagtgcgg tggtgaaaat aaagcccggc gcataggatc gcacggcgtc
     5341 gataatttcg gtcttggccg cgatatagcc gccgaggcag ccgaacgctt tcgcgagcgt
     5401 gccctcgagg acgtcgatgc gatgcatgac gccgtcgcgc tcggcgatgc cgccgccgcg
     5461 cgggccgtac atgccgaccg cgtgcacctc gtcgacatag gtcatcgcgc catagcgctc
     5521 ggcgagatcg cagatgcgcg cgagcggcgc gatgtcgccg tccatcgaat agaggctctc
     5581 gcagacgatc agcttcggcc ggtcggggcc ggcggcgcgg agcagttcct ccaggtgcgc
     5641 catgtcgctg tgccggaaga tttgccgctc gcggccggcc tgccgcacgc cttcgatcat
     5701 cgaattgtgg ttgagcgcgt ccgacaggat caggcagttt ggaatgagct ttgcgatagt
     5761 ggcaatgccg gtctggttcg agacatagcc cgaggtgaac aacagcgccg cttccttgcc
     5821 gtgcagatcg gcgagttccg cctcgagctg taccagcggg tggttggtgc cggcgatgtt
     5881 gcgggtgccg ccggcgccgg tgccgacgcg ggtagcggtc tcgaccatgg cgccgaccac
     5941 cttcggatgc tggcccatgc cgagataatc gttggagcac cagatcacga cgttgcgcgg
     6001 acccttcggc gaatgccaca ccgcatgcgg aaaccgcccg gcgatgcgtt cgagatcggc
     6061 gaacacgcga tagcgccgct cgtcgtggag ctggccgagg gcggcattga aaaattcgct
     6121 atagtccatc gaaaacctgg agtggggctg gagcagaatg gggtcggggc ctttttagag
     6181 cgtttccagc tttgctgtcg agccggaatc gggcgcggaa tcgcgcacct gtcgatccag
     6241 cattgctcaa aacgcggatc gcgacttgat caggatcaat aggtggtgaa cagaagtttc
     6301 aggtggtcct gaaacggagc acgccgtcgg tttcctggac ggtcaggcgg ccctcgacca
     6361 gcatgtaatt gacatgagcg atcagctcgc cggccgcaaa gcccatctgg tgggcgtcaa
     6421 gcgcatgctt gtggaacacc accggcacca gctccttcga agtctgcgga acatcccggc
     6481 aggcgtcggc gatcagccgg cagcgttcct cgtgatgatc ggcgagctgt ttgatgcgcg
     6541 tcttcaaccc gtagaacggc acgccatgtc ccggcagcac cagcacgtcg tagggcaatg
     6601 tggtggtgag gcttgcgagc gaagccagat attcgccgag cgagttctgg tcgggctcga
     6661 ccgcccagac gctgacattg ggcgaaatct tgctcaggac ctggtcggcg gagaggaaca
     6721 atttgtcggc cgcgcaatac agcatcacct ggtcgagcgc gtggccgccg ccggtgatga
     6781 ccttgaagcg gcgcgtgccg atggtgattt cgtcgccatg cgagatgcgg cgatagccgg
     6841 gcggcaggat cgaaacgcgc ttcaggtagt cctgaccgcg gcccaatagc ttgtcggtca
     6901 ggctttcgtc catgccgtga cggcggaaga acaggcgctg cgcgttgcgc cgctcttcgg
     6961 tgccgcggtt ctggtgatag actgattgca gatattccac ctgcgacatc acgagcggac
     7021 agccgaagcg ttcgacgatc catcctgcga gcccgacatg atcgggatgc gaatgggtga
     7081 cgatcaaccg cgtgatcctg atgtccttga gcggaccctc gatcagcgag gtccaggccg
     7141 cgatcgtctc ttcgttgccg aaaccggaat cgaccgcggt ccagccgtcg ctgtccttga
     7201 gcagatagat gttcacatga ttgagccgga acggcagctt cagccgcacc cagagcacgc
     7261 ccggcatcaa ctcgacgacc tgatcgggcc ccgggtgatt ttcgtaggga taacgcagtg
     7321 cctcggccga ggactgaatc ttgtcgctct tcgaatgcat gcaattggct tagctgcacc
     7381 ggaggcgcag cgccagctta aaaaacggcg tgccggtcgt cgttccgggg ctggcgctca
     7441 catgaagccg gggttgcgcg gcaggtaacg gctgaaatcg ccgaattcgc gagcggacat
     7501 gagtaccatc ggaaacgtga tgcgcatgcc gtgcgccgtc gcggcatcca gcgccgcatg
     7561 gttgatgccg gggagaaagg cggaaacctc cgaagtcccg gtttccgccg cgagcttgaa
     7621 tgccgtcgcg aaagccgcac tcatcaggcc gggctccgct accgccagcg gaccgatgtg
     7681 gccgccggaa tcgatgtagg cataaccgac gcaatcggtt ccggcgtaga gaaagacgcc
     7741 tgtgatgccg gcatttcccg acaggaatcc atgatgcttg tcccg
//
LOCUS       DATLJT010000767         1594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2345398,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000767 DATLJT010000000
VERSION     DATLJT010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345398"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VM910_17455"
     CDS             complement(<1..936)
                     /locus_tag="VM910_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64349.1"
                     /translation="MPRKSESKTEVAAAQIPGQVQRLSLFGPPLLLEGEDAAAYDELL
                     ARMCAAVKPVDVIDEMLVADIVALQWEVLRWRRLKRTLMRQVALKALERFLIDRLESN
                     YALHEEHFQSYLTEILKNNLPPEQADSAEMLAAECTPNTDEANDKLGKVLSSIGLGNN
                     VLDDARADKAKELAQEYVRGERDAVTLVNELLTDAGVSMDTFMTKALRKRIDEIERID
                     RLTTIAPPQCRPARDGPASRGPRRNAATPRARDRGRRVYGRRVSSCAAASRRKRGLTS
                     ERKITANRANARASTGPRTTRGRARAARNALRHALS"
BASE COUNT          272 a          509 c          475 g          338 t
ORIGIN      
        1 gctgagtgca tggcgaagcg cgtttctcgc tgcgcgagcg cggccccgag tggttcgcgg
       61 gccggtgctg gcccgcgcat tcgcgcggtt ggccgtgatc ttgcgctcgc tcgtcaagcc
      121 gcgtttcctt cggctggcgg cggcacagga actaactcgc cgtccgtaaa cccgacgtcc
      181 tcgatctctt gcacgcggcg tcgcagcgtt tcgccgagga ccgcgcgacg ccggtccatc
      241 tcgcgcaggg cggcattgcg gcggcgcgat ggtggtcagg cggtcgatcc gctcgatttc
      301 gtcgattctc ttgcggagtg ctttggtcat gaacgtgtcc atgctcacgc cggcgtctgt
      361 gagaagctca ttgacgagtg tcacggcgtc tcgttcaccc cgcacgtact cctgcgcgag
      421 ctctttcgcc ttgtcggccc gcgcatcgtc caggacgttg ttgcctaacc cgatgctgct
      481 gaggacctta ccgagcttgt cgttcgcttc gtcggtattc ggggtacatt cggccgccag
      541 catctctgca gagtccgctt gctctggcgg aaggttgttt ttgagaattt ccgtgagata
      601 actctgaaaa tgttcctcgt ggagagcgta gttcgactcg agccgatcta ttaaaaaacg
      661 ttccagtgcc ttaagtgcga cctgtcgcat caaagtccgt ttcaaccggc gccatcggag
      721 gacctcccac tgcaacgcca ctatgtcggc gacgagcatc tcgtcgatca catcgaccgg
      781 ctttaccgcc gcgcacatac gcgcgagaag ctcgtcataa gcggcggcat cctcgccctc
      841 gaggagcagc ggcgggccga acagtgataa acgctgcacc tgccccggaa tttgggccgc
      901 tgcgacttcc gtcttgcttt cactcttcct gggcacaaac gccaccatac gctcgactaa
      961 acgaatcacc gacgcacatc tcattataca tcaacgatag cggcatgacc cggcaacgcg
     1021 cgtctggaac ggccgagctc gtcgaatttc gacacctcca aaccggcatg tgcggctagc
     1081 gatgatccgc attggctagg agcaccactc gacttgggcc gtaaggcgga ccccgctccg
     1141 ggaggcagcc agcgcttctg cggaatcggc tattttgcgt cagccccgtc agcatcgtct
     1201 gcacttaaag cgcctgttcc agtccgggcc gaggatggct tagatccgct ccatgtcgat
     1261 tgtcgctgac tgtgctgacg gtgctgacgg tcatggcgat gtcttcggcc ctcgtgtaaa
     1321 ttctgatcac cctggttcca gctcggcttt cacggctgaa ggcaatctgg atgcccagcg
     1381 cccgaaggaa cggctgcgca cgacgcaatc ggccggcgag tgccctgggg ttcttcggcc
     1441 aagctggggc cccgcccggg aggccgggat ccgacagatc ggcgccggca cgcaggaggt
     1501 cggaagcatt tcccgtccac gtgctgcagt tcgccataat ttgccgtatc cgagcggcta
     1561 gtggatcaac gtcgatcaag tcctcgatcg cggc
//
LOCUS       DATLJT010000768         4980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_508183,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000768 DATLJT010000000
VERSION     DATLJT010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508183"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /gene="istA"
                     /locus_tag="VM910_17460"
     CDS             <1..1375
                     /gene="istA"
                     /locus_tag="VM910_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI64350.1"
                     /translation="ETSFEYEREVQPRPKLGRWTTDLEQLLAANAVRPTREQLTLIRI
                     FEELRGRGYDGGYDAVRRYARHWAKQHGQATAAAYVPLSFAPGEAYQFDWSHEVVLLG
                     GATVIVKAAHVRLCHSRMLFVRAYPRETQEMVFDAHDRAFALFKGACRRGIYDNMKTA
                     VETVFVGKDRQYNRRFLQMCSHYLVEPVACTPASGWEKGQVENQVGLVRERFFTPRLR
                     FKNYDELNAWLLDKCIAYAKAHRHPELSEQTIWEVFEAERPKLVPHAGRFDGFHAVPA
                     SVSKTCLVRFDNNKYSVAASTVGRPVEVHAYADRIVIRQDGRIVAEHPRSFGRGETIY
                     DPWHYVPVLARKPGALRNGAPFKDWVLPAAIERVRRKLATADDGNRQMVDILTAVLTD
                     GLPAVEAACSEAIAHGVHSADVVLNILARQRNPAPLVTILTPAALTLRHAPIADCARY
                     DNLRRTV"
     gene            1375..2256
                     /gene="istB"
                     /locus_tag="VM910_17465"
     CDS             1375..2256
                     /gene="istB"
                     /locus_tag="VM910_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI64351.1"
                     /translation="MERSQLFDLMGELQLYGMKAAFDEIMATAVKRQHDPQRIIGDLL
                     TAEISEKQARSIKYQLTIAKLPLAKDLDDFQFEDTPINETLVRDLAGGGFLAQQRNAV
                     LVGGTGTGKTHLAIAIARSCIRAGARGRFYNVVDLVNRLESEARNGHQGRLADHLTRM
                     DFVVLDELGYLPFAQSGGQLLFHLISRLYERTSVLVTTNLAFGEWPSVFTDAKMTTAL
                     LDRLTHHCDIIETGNDSWRFKSREDDHVPTRARGVSATPTSSDAASATARTRGRRGSK
                     LDADQGRIASSRMLPEN"
     gene            2302..3969
                     /locus_tag="VM910_17470"
     CDS             2302..3969
                     /locus_tag="VM910_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HVI64352.1"
                     /translation="MAGRISMGARRELTAAVADRYRSAGRADKGRILDELCAVTGWHR
                     KHAVRALAIHVAISPDARRQRRPTYGAPIRDALVALWEASDRICGKRLRVMIPTLLPS
                     LERHGRLKLDQADRALVLGVSAATIDRLLVETKIAAAGGKRRRVGFYSAVRREVPIRT
                     FNDWHDPPPGFCEVDMVAHGGTSVAGSFIQTLTMVDVATGWTECMPLVTRESGLVVRA
                     MERARNLFPWLIRGADFDNDSAFMNDVVVPWCRTQKIEVTRSRAYKKNDQAFVEQKNG
                     AVVRRLVGYGRFDGVETTRVLERLYAASRLHTNFFQPSFKLKDKRREGAKVIKRYYVP
                     ATPYARALAHPKLSKGVKRRLRELYRTLDPVALLAEMRSAQTELGTRVGARAGKLAAA
                     ASPPAPATNAAAFARGIGNDVHLGEQRIIHRRTRKPYKKRMRMPSMLDPHLADIERWL
                     AVEPRLTALAILGRLAEHSPEKFGPPQHTIVQRLLRSLRCKATETIIPCTAAGAAPAG
                     DPGTGADAACDGHSATPPVPPNLRAMMQTRTGRQSELHLSSESNILR"
     gene            4042..>4980
                     /locus_tag="VM910_17475"
     CDS             4042..>4980
                     /locus_tag="VM910_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HVI64353.1"
                     /translation="MVADPHRRPDRRKPREAGKALTVSIRLAVSGLVLAAILLTAAVS
                     SLVWWQTAESVSQRLASTINEQIVAAVRKEVSAIVDEARAAHTAIRTLFLQNVLDTRE
                     ADKREFVFLSQLQSQATISWAAFGWPDGSFFAAHKLGDRHLEMMEISLTDHPGQRRVD
                     EYEVIPGDIEFEHRHFEPTDFRVGDQAWFKTGIAAAEPQWFKVLNHPIGERPSIAYAG
                     PIDVYQERQGVLAIIIEYTRLSHFLSQLEVGRTGTAFIFDGSGGLVAAPDKDADELHS
                     AHGQETLLPLAQMALARAGDEGRKEAWRRSLTYGGEA"
BASE COUNT          946 a         1591 c         1571 g          872 t
ORIGIN      
        1 ggagacgtcg ttcgagtacg agcgcgaggt gcagccgcgg ccaaagttgg ggcgatggac
       61 caccgacctc gagcaactgc tggcggccaa tgcggtacga cccacgcgcg agcagctgac
      121 gctgatccgg atcttcgagg aactgcgcgg gcgcggctat gacggcggct acgatgcggt
      181 gcgccgttac gctcgacact gggccaagca gcacggtcag gcaacggcgg cggcctatgt
      241 cccgctgagc tttgcgcccg gcgaagccta tcagttcgac tggagccacg aagtcgttct
      301 gctcggcggc gcaaccgtga tcgtcaaagc agctcatgtc cggctctgtc acagccggat
      361 gttgttcgtg cgcgcctatc cccgcgagac ccaggagatg gtgttcgacg cccacgaccg
      421 ggccttcgcg ctgttcaagg gggcctgccg gcgcggcatt tacgacaaca tgaagaccgc
      481 agtggagacg gtcttcgtcg gcaaggaccg gcagtataat cggcgctttc tgcagatgtg
      541 cagccattac ctggtcgagc cggtggcctg cacgccggcg tcgggctggg agaagggcca
      601 ggtcgagaac caggttgggc tggtccgtga gcgcttcttc acaccgcggc tgcggttcaa
      661 aaattacgac gagttgaacg cctggttgct cgacaaatgc atcgcctacg ccaaggcaca
      721 tcgccatccc gaactgagtg agcagaccat ctgggaggtg tttgaggcgg aacgcccgaa
      781 gctcgttccc catgccgggc ggttcgacgg cttccatgcg gtaccggcct cagtctccaa
      841 gacctgcctg gtccgcttcg acaacaacaa gtactcggtg gcggccagca cggtcgggcg
      901 accggtcgag gttcacgcct atgcagatcg catcgtcatc cgccaggacg gccggattgt
      961 tgccgagcat cctcgctcgt ttggccgcgg cgagacgatc tacgacccct ggcattatgt
     1021 cccggtgctc gcccgcaagc ccggcgcctt gcgcaacggg gcgcccttca aagattgggt
     1081 gctgccggcc gcgatcgagc gcgtgcggcg caaactcgcc actgccgacg acggcaatcg
     1141 gcagatggtc gacattctta ccgcagtgct gaccgacggg ttgccggcgg tcgaggcggc
     1201 ctgcagtgag gccattgccc acggcgtcca ctccgctgac gtcgtcctca acatcctggc
     1261 ccgccaacgt aatcccgcgc cgctagtcac catcctcacg ccggccgcgc tgacgctgcg
     1321 ccacgccccg atcgccgact gtgcccgtta cgataacctc aggagaaccg tgtgatggaa
     1381 cgatcccagc tcttcgacct catgggcgaa cttcagctct atggcatgaa ggccgccttc
     1441 gacgagatca tggccaccgc cgtgaagcgc cagcatgatc cccagcgtat catcggtgat
     1501 ctgctcaccg ctgagatcag cgagaagcag gcgcgctcga tcaaatacca gctcaccatc
     1561 gccaagttgc cgctcgccaa ggatctcgat gacttccagt tcgaggatac gccgatcaac
     1621 gagacccttg tgcgcgatct cgctggcggc ggattcctcg cacagcagcg caacgccgtg
     1681 ctagtcggcg gcaccggcac tggcaagact cacctcgcca tcgcgattgc ccgcagctgc
     1741 atccgcgctg gcgcccgcgg ccggttctac aacgtcgtcg atcttgttaa ccggctcgaa
     1801 tccgaagccc gcaacggcca ccagggacgg ctcgccgatc acctgacccg gatggacttc
     1861 gtcgttctcg acgagctcgg ctatctgccg ttcgcccagt ccggcggcca gctgctgttc
     1921 caccttatca gccggctcta cgagcgcacc tcggtgctcg tgaccaccaa tctggcgttc
     1981 ggcgaatggc cgagcgtctt cacggacgcc aaaatgacga ccgcgctgct cgatcgcctg
     2041 acccatcact gcgatatcat cgaaaccgga aacgacagtt ggcgcttcaa gagccgcgaa
     2101 gacgatcacg tcccaacccg cgctcgcggc gtctccgcaa ccccgaccag ctccgacgcc
     2161 gcaagcgcta ccgccaggac tcgtggcagg agggggtcaa aattggacgc cgatcagggg
     2221 cgcattgcct catcaagaat gttaccggag aactgaccgt caggattggg gcggcgatac
     2281 cgaatcggat cggtgtcgcc gatggcagga cggatcagca tgggtgcgcg gcgcgagctc
     2341 acggcggcgg tggcagaccg ctatcgatct gcagggcggg cggataaggg gcgcattctc
     2401 gacgagctgt gcgcggtgac gggctggcat cgcaagcacg cggttcgggc gcttgccatc
     2461 catgtcgcaa tttcgccgga tgcccggcgg cagcgaaggc cgacctatgg cgcccctatc
     2521 agggatgcgc tggtggcgct gtgggaggcc tcggatcgga tctgcggcaa gcggctcagg
     2581 gtaatgattc cgacgttgct gccttcgctg gagcggcatg gccggctcaa gctcgaccag
     2641 gccgatcgag cgctggtgct cggcgtcagc gcggccacga ttgatcgcct gctggtcgag
     2701 accaaaattg ccgcggccgg cggtaagcgc cggcgggtcg gcttttattc ggcggtacgg
     2761 cgtgaagttc cgatccggac gttcaacgac tggcacgatc cgccgccggg gttctgcgag
     2821 gtcgacatgg tggctcatgg cggcacatcc gtggctggct cgttcattca gacgttgacg
     2881 atggtcgacg tcgcgactgg ttggaccgaa tgcatgccgc tggtgacgcg cgagagcggc
     2941 ctcgtcgtgc gagccatgga gcgcgcacgg aacctgtttc cctggctcat tcgcggcgcc
     3001 gacttcgaca acgacagcgc gttcatgaac gatgtcgtcg tgccctggtg tcgcacgcaa
     3061 aagatcgagg tgacacgctc gcgcgcctac aagaagaatg atcaggcttt cgtcgagcag
     3121 aagaatggcg cggtcgtccg gcgacttgtg ggatacggtc gcttcgatgg cgtcgagacg
     3181 acacgcgtgc tggaacgctt gtatgccgcg tcgcggctgc acaccaactt cttccagccg
     3241 tccttcaagt tgaaagacaa gcgacgcgag ggcgcgaaag tgatcaagcg ctattatgtt
     3301 ccggccacgc cctatgcgcg cgcactggcg catccaaagt tgagcaaagg cgtcaaacgg
     3361 cgtcttcgag agctttatcg cacgctcgat cccgtggccc tgctggccga gatgaggagc
     3421 gcccagacgg agctcggcac gcgcgtcggc gcccgagcgg gcaagctcgc ggcagctgcc
     3481 agcccaccgg ctccggcaac gaacgcggca gcgttcgcga gggggatcgg caatgatgtg
     3541 catctggggg agcagcgcat catccatcgg cgcacgcgca agccatacaa aaagcgcatg
     3601 agaatgccat cgatgctcga tccacacctc gccgacatcg agcgctggct cgccgtcgaa
     3661 cctcgtctga ccgcgctggc catcctcggt cgtctcgccg aacactcccc cgaaaagttc
     3721 gggccgccac aacacaccat agtgcagcgc ttgttgcgat cgctcagatg caaagcaacc
     3781 gagacgatca tcccctgcac cgctgcgggt gcggcaccgg ccggtgatcc cgggactggt
     3841 gccgacgctg cgtgtgatgg gcactccgcg acgcccccag tccctccgaa cctgcgagct
     3901 atgatgcaaa ctcggaccgg ccgacagtcg gagctacatc tgtcaagcga aagtaacatc
     3961 ctcagatgag gcaatacggg gggtcaaaat tgcacgccga ataacaaggt tccaagcgga
     4021 tctggaggac tgagggcaaa gatggtcgct gatccccatc gaagacctga tcgacgcaag
     4081 cctcgcgagg ctggaaaagc gctaacagtt agcatccgtc ttgcggtgtc gggacttgtg
     4141 ttggcagcaa ttctgttgac cgccgcggta tccagcctcg tctggtggca aacggcagaa
     4201 tccgtcagcc agcggctcgc cagcaccatc aacgagcaga tcgtcgctgc tgttcgcaag
     4261 gaagtgtccg cgatcgtgga cgaagcgcgc gccgctcaca ctgcgattcg taccctcttc
     4321 ctacagaacg tgctggatac gcgcgaggcg gacaagcgcg agttcgtctt cctgtcgcaa
     4381 ctgcagtcgc aggcgacgat atcgtgggca gcattcggct ggcccgacgg ctcgttcttt
     4441 gccgcccaca agctcggcga tcgccacctc gagatgatgg agatctcact gaccgaccat
     4501 cctggtcagc gccgcgtcga tgaatatgag gtgatcccgg gtgacatcga gtttgagcat
     4561 cgccacttcg agccaaccga ctttcgggtc ggcgaccagg cgtggttcaa gaccggtatc
     4621 gcggcggcgg agccgcaatg gttcaaagtt ctcaatcacc ccatcggcga gcgtccgtcg
     4681 atcgcttatg cgggacccat cgatgtttat caggagcgac agggcgttct tgcgatcatt
     4741 atcgaataca cacgcctttc gcatttcctc tcgcagctcg aggtgggacg caccggaacg
     4801 gcattcatct tcgacggcag cggcgggctt gtagcggctc cggacaaaga tgccgacgaa
     4861 ttgcattcgg cgcacggtca agagacactg ctaccgctgg cgcaaatggc cctcgcgcgc
     4921 gcaggcgacg agggccggaa ggaagcatgg cgacgcagtc ttacgtacgg cggtgaggcc
//
LOCUS       DATLJT010000769         1997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3495163,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000769 DATLJT010000000
VERSION     DATLJT010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495163"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VM910_17480"
     CDS             <1..393
                     /locus_tag="VM910_17480"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVI64354.1"
                     /translation="PAIHPNYLATEKDRETIVAGTKALRRIFRAPAMARHIAEEIEPG
                     DQCQRDDELLDFIRRRGSTTYHPVSTCRMGQDAAAVTDERLRLHGFAGLRVVDASIMP
                     AVVSGNTNAATIMIGEKGADMILKDASR"
     gene            417..491
                     /locus_tag="VM910_17485"
                     /pseudo
     CDS             417..491
                     /locus_tag="VM910_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(1125..>1997)
                     /locus_tag="VM910_17490"
     CDS             complement(1125..>1997)
                     /locus_tag="VM910_17490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI64355.1"
                     /translation="QVSATLTLLTWLAARTTTLRLGTAVMVLPWHNPVLLAEQAATLD
                     LLSKGRLDFGVGKGYRYNEFTGFAIPFDEADARFEEALAVITKAWTSGQRFSHHGRFW
                     TFNDILVEPPTSQKPHPPFWMAAGTPPSIRRVAERGYNLLLDQFAAFDVIGERISIFK
                     AERAACGRAYNPMGIALARDMHVANNQAAKDDALERNRQFHERILAVARWPDRAVGSH
                     ILAYSHAPEEAEANSLVGTPDEIMGKLEGLRRAGIEYVILSCGGSRESLRRFAHEIMP
                     AFSDEPAKVRANPV"
BASE COUNT          363 a          636 c          637 g          361 t
ORIGIN      
        1 ccagcgatcc atcccaatta tctcgcgacc gagaaggacc gagagaccat cgtcgccggc
       61 accaaggcac tgcggcgcat cttccgcgca ccagccatgg cgcgccatat cgccgaggag
      121 atcgagcccg gcgatcagtg ccagcgcgac gacgagctgc tcgatttcat ccgccgccgc
      181 ggctccacca cctaccatcc cgtcagcacc tgccggatgg gccaagacgc ggcggccgtg
      241 accgacgaac ggctgcggtt gcacggcttt gcagggcttc gcgtggtcga tgcctcgatc
      301 atgccggcgg tcgtgtccgg caacaccaat gcggccacga tcatgatcgg cgagaagggt
      361 gccgacatga tccttaagga cgcctcccga tagatcgctt gcgggtgccg cccgtgcgcc
      421 agccctatct gccgcgcttc atggccggcg gcgagaccaa cccgctcggc gcgcgcgctc
      481 tctatcttgg caagtaaccg ccgcagcgac ttcgctcgcg ttactcgcgg ccatgccgtc
      541 gcaggcggcc gtgcccgccc cgccgtccgt gatggcacga tgaataatcc atcatcccca
      601 ctggcggtcc gtcgggcagc cagtcggcgt tgctcgacga tcagcgcgaa ccgctaggtc
      661 aggtcattgc cgcggccatc gggcgttggt ttgccgcacg aagacatctg gcagctcgta
      721 atctcaccgc gcactaaccg cggcattgac cgggagacgc tgcgctcggc atttcgtctt
      781 gagcttgacc gggccgcgca gtagcgggcc gggccgttgc gaacgcgctt acaacaacgc
      841 cgttcacaat aacaacgtgg cggcacaaat ccggtggagc aaggcgcgca tgcgctggaa
      901 gcgaaacggt cggtcatcag atgcttgatg aagagggcaa tccgacgtgg ccggtcatta
      961 actacatcgg gcggccgcag tctgacgacg gcgagagcga atagccacgt atcagattcg
     1021 ttatctcgct gttctctgcg accgaatgtt aggttttgtg acgttcggcg taagactgca
     1081 tgacacgcgc acgcacgcgt acgtgtgcaa attgccggac aactttatac ggggttggct
     1141 ctgactttcg ctggttcatc ggaaaaggcc ggcatgatct cgtgcgcgaa acgacggagg
     1201 ctctcccgcg agccgccgca gctcaaaatc acgtattcga tgccagctcg acgcaagcct
     1261 tcgagcttgc ccatgatctc gtctggcgtg ccgaccaggc tgttcgcctc tgcttcttcg
     1321 ggcgcatgac tataggctag gatatgcgat ccgacggcgc ggtccggcca acgcgccacc
     1381 gccaaaatgc gttcatggaa ctggcggttg cgttcgaggg cgtcgtcctt ggcggcctgg
     1441 ttgttggcga catgcatgtc gcgcgcaagg gcaatcccca ttggattgta ggcacgcccg
     1501 cacgccgcgc gctcggcctt gaatatgcta atgcgctcgc cgatgacgtc gaacgctgcg
     1561 aactggtcga ggagcagatt gtagccgcgc tcggccacgc ggcgaatgga cgggggggtc
     1621 ccggcggcca tccagaacgg cgggtgcggt ttttggctgg tcggcggctc gaccaggatg
     1681 tcgttgaagg tccagaaccg gccgtggtgc gagaagcgtt gccctgaagt ccatgctttg
     1741 gtgatgaccg caagcgcctc ctcgaagcgc gcatcggcct cgtcgaatgg gatcgcgaag
     1801 cccgtgaact cattatagcg ataacctttg ccgactccga aatcgagccg gcccttcgac
     1861 aggaggtcca aggtcgcggc ctgctcggcc aggagcactg gattgtgcca cggcagcacc
     1921 attacggcag tgccgagccg cagcgtcgtg gtgcgcgccg cgagccacgt cagcagggtg
     1981 agggtggccg agacttg
//
LOCUS       DATLJT010000770         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_48553,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000770 DATLJT010000000
VERSION     DATLJT010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48553"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VM910_17495"
     CDS             <1..1897
                     /locus_tag="VM910_17495"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64356.1"
                     /translation="DAKATDAAFAKAAHVVALELINNRVVVNSMEPRNAVADYDPQSG
                     RSTLYTATQGPHFVRDPLAELVLKMPKDMLRLITPNVGGGFGMKAFVYPEHALVVWAS
                     RKLKRPVKWQEDRSEAFISDNQGRDHAARAELALDENGRFLGLRVSILANLGAYLSPF
                     GSFVPTRSTDLVSGLYAIGAIHINVKGICTNTVPVCAYRGAGRPEAAYLLERLVDAAA
                     RDLGMSPDRIRRVNFVPSSAMPYTSATKLLLDSGEFEKVMDRCMAAAEWPSFARRRTQ
                     SERNGKLRGIGMATYTERCGGGFPETASIEFKDDRVELIMGNQEYGTGLLTAYKQLVS
                     EQLGIDADRIDIVMGDTDRTPSGLTGGSRALAVAGSALYEAGLAIIEKGRQLAAHVLE
                     VSAQDVSFNDGVFGVPGTDLHLNIFEAAKAARDPAKLPPGMATGLDTTHTRSPPAQTF
                     PNGCHTVEVEIDPNTGAVTIERYQPAAARGSGAWRRGARCRSGTARTCDLRRRERATS
                     VRLVHGLLDAAGRRCPVIRLLDPQRTDERQSARRQGRRRSGRSGRAAGGDECDHRCAR
                     PSRWRAPHRYAGNTGARVGGSQWRVRQDRVLRPLRRPKRARAPAKSLEIMISRTAGPQ
                     PRNFSAG"
BASE COUNT          379 a          646 c          626 g          381 t
ORIGIN      
        1 ggacgccaag gcgaccgatg ccgccttcgc caaagcagcc catgtggtcg ccctcgaact
       61 catcaacaac cgcgtcgtgg tcaactcaat ggagccacgc aatgcggtcg ccgattacga
      121 tccgcagagc ggacgctcga cgctctacac agcgacgcaa ggtccacatt ttgtacgtga
      181 cccgctggct gagctcgtgc tcaagatgcc gaaagacatg cttcgcctga tcacgccgaa
      241 tgtcggcggc ggctttggca tgaaggcctt cgtctatccc gagcacgcgc tggtggtctg
      301 ggccagccgc aagctcaaac ggccggtgaa atggcaggag gaccgctccg aggccttcat
      361 ctccgacaat caagggcgcg atcacgcggc gcgcgcggag cttgcactcg acgagaacgg
      421 ccgctttctc ggtctgcgcg tctccatcct tgccaatctc ggcgcctacc tgtcgccgtt
      481 tggctctttc gttccgactc gctcgaccga tcttgtctct gggctctatg cgatcggggc
      541 catccacatc aatgttaaag gcatatgtac caataccgtg ccggtctgcg cctatcgtgg
      601 tgccggtcgc cccgaggccg catatctgct cgagcgtctg gtcgatgcgg ccgcacgcga
      661 cctcggcatg agccccgacc gcattcggcg cgttaatttc gtgccgtcct cggcaatgcc
      721 ttacacttcg gcgacaaaac ttcttctcga ttctggtgag tttgaaaagg tgatggaccg
      781 ctgcatggcc gcggcggaat ggccgtcctt cgcgcggcgg cgcacgcaaa gcgagcgtaa
      841 cggcaaattg cgcggcatcg gcatggcgac atataccgag cgctgtggcg gcggatttcc
      901 ggagaccgcc tcgatcgagt tcaaggacga tcgggtcgag ctcataatgg gcaaccaaga
      961 atacggcact ggtcttctca ccgcgtataa gcagctcgta tcggagcaac tcggcatcga
     1021 tgccgaccgg atagatatcg tcatgggcga caccgatcgt acgccgtctg gactgacagg
     1081 aggctcgcgc gccctcgctg tcgccggcag cgccctctac gaagccggac tcgcgatcat
     1141 cgagaagggc aggcagctcg ccgcgcatgt tctcgaagta tcggcccaag acgtcagctt
     1201 taacgacggg gtgttcggcg tgccgggcac cgatcttcat ctgaacattt tcgaggcggc
     1261 gaaggcggcg cgagatcccg ccaaactgcc gccgggcatg gcaaccggtc ttgacacaac
     1321 acatacgcgc tcgccgccgg cacaaacttt cccgaacggc tgccacaccg tcgaggtcga
     1381 gatcgatccg aataccgggg cagttaccat tgaacgctat caacccgctg ctgctcgagg
     1441 gtcaggtgca tggcggcgtg gtgcaaggtg tcggtcaggc actgctcgaa catgcgattt
     1501 acgacgccga gagcgggcga cttctgtccg gctcgttcat ggattactcg atgccgcggg
     1561 ccggcgatgt cccgtcattc gccttctcga cccacaacgt accgacgagc gccaatccgc
     1621 tcggcgtcaa gggcgccggc gaagcgggcg cagtgggcgc gccgccggcg gtgatgaatg
     1681 cgatcatcga tgcgctcgcc cgtcgcgttg gcgtgcgcca catcgatatg ccggcaacac
     1741 cggcgcgcgt gtgggaggtt ctcaatggcg cgtccgccag gatcgcgtgc tccgtccgct
     1801 gcgaaggcca aaacgggccc gcgcacccgc taagtccttg gaaatcatga ttagccgcac
     1861 cgcgggtccg cagccccgaa atttctcagc cggctgaggg aactttgcgc tggggccgcg
     1921 actttagatg ggcggattct tcggggatct cccatggcca ctgtgttgct tgttgaagac
     1981 gacgatcagg tccgcgtgct ggccgaatcg tatctggaag aacagggaca cg
//
LOCUS       DATLJT010000771         1708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2576480,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000771 DATLJT010000000
VERSION     DATLJT010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576480"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VM910_17500"
     CDS             <1..318
                     /locus_tag="VM910_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI64357.1"
                     /translation="YSARFTIENPDDTVALGMTATVKLARPATGRVAKLPLSAILNRG
                     TGPSVYVVEGPGALVLRPVTIASFTEDAALVSAGVADGDKVVTLGVQKLEPGTRVRTV
                     DAR"
     gene            360..>1708
                     /locus_tag="VM910_17505"
     CDS             360..>1708
                     /locus_tag="VM910_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI64358.1"
                     /translation="MKRFNLSEWSITHQALVLFMIILLGAAGVYSYLNLGRAEDPNFT
                     IKVMVIGVGWPGATADEVQTQVTDKIEKKLQELPYLDRVETYSQPGGAFLQVILADTT
                     PPAKVKDLWYQVRKKVGDIKNELPAGITGPNFNDEFGDVYSALYMLTADGLTLAQLKD
                     RAEDIRQRLLRIPDVNKVDIIGERPQKIFIEFSHTKLATLGVTPQQIFDSVARQNAVV
                     SGGSVDTSADRINLRVTGAFSGVAAIAAVPVHANGQIFRLGDIATVKRGYEDPPSFIL
                     RQGGKPALGLGVSMQEGANILTLGKNLQQAMTAITAELPLGVEVTQVADQPRIVDGSV
                     SEFVETFAEALVIVLAVSFLSLGWRTGIVVALSVPLVLAIVLLVMYASGLVLHRITLG
                     ALIIALGLLVDDAIITIEMMVVKMEQGWDRARAATFAWTSTAFPMLTGTLVTAAGFLP
                     "
BASE COUNT          312 a          535 c          525 g          336 t
ORIGIN      
        1 tattcggcgc gcttcaccat tgagaatccg gacgacacgg tcgcgctcgg catgaccgca
       61 acagtgaagt tggcacggcc tgcgaccggt cgcgtcgcca agctgccgtt atccgcaatc
      121 ctcaaccgtg gaacgggacc ctcggtctat gtggtcgagg gaccgggtgc actggtgctg
      181 cggcctgtaa cgatcgcgtc cttcaccgag gatgcggcgc tagtcagcgc cggcgtcgcc
      241 gacggcgaca aggtcgtcac cctcggtgtg cagaaactcg agcccggtac gcgcgtccga
      301 accgtcgacg cgcgctaaca agcgaacttg ctcccacccg cgaatggaaa gggcgcacca
      361 tgaaacggtt taatctctct gaatggtcga tcacgcacca ggcgctggtg ctgttcatga
      421 tcattctctt gggcgccgcc ggcgtctatt cctacctcaa ccttggacgt gcggaggatc
      481 caaacttcac catcaaggtc atggtgatcg gcgtcggctg gccgggagcg accgcagacg
      541 aggtgcagac gcaggtcact gacaagatcg agaagaaact gcaggagctg ccatatctcg
      601 accgtgtcga gacttattcg cagccgggcg gtgccttcct tcaggtgatc ctggcggata
      661 ccacccctcc cgccaaggtg aaggacctct ggtatcaggt ccgaaaaaag gtcggcgata
      721 tcaagaacga gcttcccgcc ggcatcactg gcccaaattt caacgatgaa ttcggcgatg
      781 tctattcggc gctctacatg ttgacggccg acggcttgac cctcgcccag ctcaaggacc
      841 gcgctgagga cattcgccag cggttgttgc ggatcccgga tgtaaacaag gtcgacatca
      901 tcggcgagcg accgcagaaa attttcatcg agttcagcca caccaagctc gccacgcttg
      961 gtgtcacccc gcagcagatt ttcgacagtg tcgcgcggca gaacgcggtt gtgtctggcg
     1021 gatcggtcga tacctcagct gatcgcatca atctgcgggt gaccggcgcg ttctcgggcg
     1081 ttgccgccat cgcggcggtg ccggtgcacg cgaatggaca gatcttccgt ctcggcgata
     1141 tcgcaaccgt aaagcgcggt tacgaggacc cgccgagctt catcctccgc cagggcggca
     1201 agcctgcgct cggcctcggc gtctccatgc aggaaggcgc caatatcctg acgctgggca
     1261 agaacctcca gcaggccatg accgcgatca ccgccgaact gccgctcggc gtcgaggtca
     1321 cgcaggtcgc cgatcagccc cgcattgtcg acggctcggt ttccgaattt gtcgagacgt
     1381 ttgcagaggc gctggtcatc gtgctcgcgg tgagctttct ctcgctcggc tggcgcaccg
     1441 ggatcgtggt cgcgctgtcg gtgccgctgg tcttggcgat cgtgctcctc gtgatgtatg
     1501 cgagcggtct cgtcctgcat cgcattacgc tcggcgcgct cattatcgcg ctcggcctgt
     1561 tggtcgacga cgcgatcatt accatcgaaa tgatggtggt gaagatggag cagggctggg
     1621 atcgcgcgcg agccgccacg tttgcctgga cctcgaccgc gttcccgatg ctgactggta
     1681 ctctagtgac cgcggcgggc ttcctgcc
//
LOCUS       DATLJT010000772         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_48645,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000772 DATLJT010000000
VERSION     DATLJT010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48645"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VM910_17510"
     CDS             complement(<1..295)
                     /locus_tag="VM910_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI64359.1"
                     /translation="MPTVSDPDLVPFEPPDHPTIRAAFRNIIESYPRALYDEQGLVRY
                     RTRFLDSVLVSEPELIHDILVARAHLFRRDDVARQLLSPMLGPTSLFMAEGADW"
     gene            complement(550..1020)
                     /locus_tag="VM910_17515"
     CDS             complement(550..1020)
                     /locus_tag="VM910_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HVI64360.1"
                     /translation="MRLNSTAFADGSAIPRRFTCDGEDLSPPLNWSDAPTGTRSFALL
                     CDDPDAPAGTWHHWAAYDIPATLTMLALGAASDAEKRLGSSRQSMIFNGPATAAHARR
                     VATACISLHHYHFRLLALSVDHLPIGGTPSCREVEREARRHTFAEGTLVGVYKR"
     gene            complement(1631..1837)
                     /locus_tag="VM910_17520"
     CDS             complement(1631..1837)
                     /locus_tag="VM910_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64361.1"
                     /translation="MMFHPRVAQAIGSALAREMEMALAPVAGTRLLAPFRMLVVSVLA
                     NLVIEANQFEVTAQASTVADPKAQ"
     gene            2051..>2294
                     /locus_tag="VM910_17525"
     CDS             2051..>2294
                     /locus_tag="VM910_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64362.1"
                     /translation="MMMSGWIKVGLEFIKALAGQGKGSSHPNAPSETVDTMAEEEPVA
                     HSVVVRPESHQIDITSEASPNKQEIERRREVVRQFFN"
BASE COUNT          471 a          669 c          724 g          430 t
ORIGIN      
        1 tccaatccgc accttccgcc atgaacaacg aggtggggcc gagcatcggt gacaacagct
       61 gccgcgccac gtcgtctctg cgaaagaggt gggcccgcgc cacaagaatg tcgtggatga
      121 gctccggctc gctgacaagg actgagtcca agaaccgagt tcggtagcgc accaagccct
      181 gctcatcata gagcgcgcgg ggatagctct cgatgatatt gcgaaatgct gcgcggatgg
      241 tcggatgatc cggcggttcg aagggaacga gatcgggatc ggacacggtt ggcatcaaaa
      301 gtccacgtta tatcgatacg gccggtccat tgcggcctct gcgaggcggc agttatcgac
      361 gttgacccac ggcttgacac accgccaccg gtcaagggcg cgtggtcata aacagtgcag
      421 gcgcagacta cgaatccttc gccgtctcag ccccctgccg ttgtagaggt aggctgcctg
      481 ctgcgtcgga gttggagccg ccactcaccg tgtggcccgt acacgggggc gttcagaact
      541 gcagacccgt catcgcttgt aaaccccaac aagggtccct tcggcaaagg tatgcctgcg
      601 tgcctcgcgt tcgacctcgc gacaggacgg tgtgccgccg atgggaaggt ggtcgaccga
      661 gagcgcgagc aagcgaaaat gataatgatg caggctgatg caggccgtgg ccacgcggcg
      721 ggcatgggcc gccgtagccg ggccgttgaa aatcattgat tgcctgcttg aacccaagcg
      781 cttctcggca tcgcttgccg cgcccagagc aagcatcgtc agagtggctg ggatgtcata
      841 ggctgcccag tgatgccagg tgccggccgg cgcatcggga tcatcgcaga gaagggcaaa
      901 gctgcgggta ccagtcggcg catcgctcca gttcaaggga ggcgacaggt cctcgccatc
      961 gcacgtgaag cgtcgaggga ttgccgaccc gtcggcaaag gcggtcgaat tcagtcgcat
     1021 gagtgtctcc cccgcgtccc aggcggagat tgctggtcta attagacaga caggaacagg
     1081 gcatgtcgca attgtacgcg tgcactcgcc ttataaggaa tgcgctaaat aaggaatgcc
     1141 ctaaacatcc tatgaaacga gcactcaaaa caagtccaaa ccaccgcgta cgcgcgatgc
     1201 tggcgcgaag ggctggccgc gctatgcggc ggggggcggt ggtggcggcc tcggtggcct
     1261 cgggggcggc ggcttcggtg gctttggcag cagcatggag gcaacccatt cccggttatg
     1321 gccgccgggc ggcggcgtga gacctgacca tcctacaccc tcggggcgtc ctccagtcct
     1381 acacgatgcc accgactacg gcgtcggcga gcacgtcgaa atcgtcgtcg ttccattcgt
     1441 tggagaggcg agaaaccgag gcgcgcttga ggaccaacgt cacgacccaa aggctacgat
     1501 cggcctgtgt cgcttcacgt cggcggtcga gagcagcgtg ggcagcaaag tgagtcccat
     1561 cctagcggtc tcggcgttga tgaccgaatc gactttggtc gatacgcatc cgtcaggctc
     1621 gctgcgatcg tcactgcgcc ttcgggtcag caaccgttga agcttgcgca gttacttcga
     1681 actggttggc ctcgatcact aggtttgcga gcacgctcac aaccaacatc ctgaacggcg
     1741 ccagcagccg cgtgccggcg accggtgcga gcgccatctc catttcgcgg gcgagtgcgg
     1801 agcctatggc ttgagcgacg cgcggatgaa acatcatctt tgatccgtcc tttgagcgcc
     1861 acaccggctg cggcgccgtt caagggctct agtctgctgc tttgcttcgc ttgttgtctg
     1921 tactcagtgc agaataatcg gcgtggcaat gcggccggac tggtcgccgc tggcctcgtt
     1981 ttcccgcgat cgattgtagg cgattgcacc accgatgcac aaagatctaa atttacggta
     2041 ggggctcagt gtgatgatgt caggctggat caaagtcgga ctcgagttca tcaaggcact
     2101 ggccggccag gggaagggca gttctcatcc gaacgcaccc agtgaaactg tggataccat
     2161 ggcagaggaa gagcctgtcg cccatagcgt tgtggtccgg cccgagagcc accaaataga
     2221 cattacatcc gaagcttctc ccaacaagca agaaattgag cgtcgccgcg aagttgtgcg
     2281 acagtttttc aatg
//
LOCUS       DATLJT010000773         4617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4184342,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000773 DATLJT010000000
VERSION     DATLJT010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184342"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VM910_17530"
     CDS             <1..607
                     /locus_tag="VM910_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI64363.1"
                     /translation="GALYLVVGVLAALLESTRSGKGQVVDTAMCDGAASLMSMFFDMA
                     AIGRWVEGRERNFLDGGAHFYGIYECACGNFISIGSIEPQFYALLRQHAGLSDPAFDA
                     QMDRKAWPELKATLVEVFKRKTREEWCKIMEGTDICFAPVLTMAEAPKHPHMAARNVF
                     VERHGVTQPAPAPRFSRTPSAIREPVAVELSAVVSEWKAGR"
     gene            complement(656..1774)
                     /gene="hppD"
                     /locus_tag="VM910_17535"
     CDS             complement(656..1774)
                     /gene="hppD"
                     /locus_tag="VM910_17535"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVI64364.1"
                     /translation="MGPFPHDAPPATISADNPMGTDGFEFVEYAHPEPAKLHALFKLM
                     GFVAVARHRTRKITVYRQGDVNYIVNEEPESHGFEFVAAHGPCAPSMAFRVVDAKRAF
                     ERALALGAEAADATAAHKTLDVPAIKGIGGSLLYFVDRYGAKGSPYDAEFEWLGSRDP
                     RPAGAGLYYIDHLTHNVHRGRMNVWAGFYERLFNFRQIRFFDIEGRASGLFSRALTSP
                     DGKIRIPINEDAGDSGQIEEYLAIYRGEGIQHIACGARDVYSTVEGLRAAGLQFMPSP
                     PDTYFEKIDTRLPGHGEDITRLRRNGILIDGQGVLDGGHTKVLLQIFSANAIGPIFFE
                     FIQRKGDDGFGEGNFKALFESIEEDQIRRGVLKVEHAA"
     gene            1893..2363
                     /locus_tag="VM910_17540"
     CDS             1893..2363
                     /locus_tag="VM910_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI64365.1"
                     /translation="MTSVDAFDLKMLAALQDDGRLTNQELAELVGLSASQCSRRRMRL
                     EEEKVIAGYHADLSGEALGFNLIAFIHITLATHSPDNAKKFRALINRVDDIQEAYSLT
                     GDADYVLKAVLRDLRSLSDIVNNVLMPHPSVAHVRSSIVLDRLKESSRLPLRAR"
     gene            2448..2759
                     /locus_tag="VM910_17545"
     CDS             2448..2759
                     /locus_tag="VM910_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HVI64366.1"
                     /translation="MALQLRPNCEYCDKDLAPNAQDARICSYECTFCADCVESKLFNV
                     CPNCGGGFVPRPIRPSREWRRGVSVATHPPSDKRVHLKYSLDEVAARAARIKDIPPEA
                     R"
     gene            complement(2767..4038)
                     /gene="fahA"
                     /locus_tag="VM910_17550"
     CDS             complement(2767..4038)
                     /gene="fahA"
                     /locus_tag="VM910_17550"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVI64367.1"
                     /translation="MPHPNDPKLRSFIAVDPSSDFPIQNLPYGVFSAKDGLAPRVGVA
                     IGNYVLDLWQLAQDCRIDVVEPAVFAAPTLNPFMALGPKVWTTTRARISELLREDHPE
                     LRDNQELRRRALVPMRDVRLHLPITVSGYTDFYSSKEHATNVGVMFRGKDNALQPNWL
                     HMPIGYNGRASTVVVSGTGVRRPRGQLKPPNVETPSFGPCKRLDFELEMGVVVGQSSP
                     MGEMLSEKQAEQMIFGFVILNDWSARDIQQWEYVPLGPFQAKAFATSISPWVVTREAL
                     EPFRLRGPTQDPAPLPYLRQTSANNFDLELDVSLRAAGMNEAKTVCRTNFKYMYWSSV
                     QQLVHHAACGCAMNVGDLLGSGTISGPEKHQRGSLLEISWNGSEPVEFGGGVKRSFLE
                     DGDSLAMRGWCQGDGYRVGFGEVEGTILPAE"
     gene            complement(4089..>4617)
                     /locus_tag="VM910_17555"
     CDS             complement(4089..>4617)
                     /locus_tag="VM910_17555"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HVI64368.1"
                     /translation="IDVVAWHGNYAPYKYDLRTFSPVGAIGFDHPDPSIFTVLTSPSE
                     TAGTANIDFVIFPERWMVAENTFRPPWYHMNIMSEFMGLIYGVYDAKPEGFVPGGMSL
                     HNCMLPHGPDRQAFDHASNGELKPVKLTGTLAFMFETRYPQRVTAHAAKSSTLQDDYA
                     DCWKGLEKRFDPSRP"
BASE COUNT          887 a         1447 c         1418 g          865 t
ORIGIN      
        1 cggggcgctc tatctcgtgg ttggcgtgct tgccgcacta ttggaatcca cgaggtccgg
       61 caagggacag gtggtggaca ccgcaatgtg cgacggcgcg gcatcgctga tgtcgatgtt
      121 cttcgacatg gccgcgatcg gccgctgggt cgaggggcgc gaacggaatt ttctcgatgg
      181 cggcgcgcat ttctacggga tctacgagtg cgcttgcggc aatttcatct cgatcggatc
      241 gatcgagccg caattctacg cgctgctgcg ccagcatgcc gggctctcgg atcccgcttt
      301 cgacgcacaa atggaccgca aggcctggcc ggagttgaag gcgacgctgg tcgaagtttt
      361 caagcgcaag acgcgcgagg aatggtgcaa gatcatggag ggtaccgaca tctgctttgc
      421 gccagttctc accatggcgg aagcgccaaa acatccgcac atggcggcgc gcaacgtctt
      481 tgtagagcgt cacggcgtga cccagcccgc acccgcgcca cgcttttcgc gtacgccatc
      541 ggcgatccgc gagccggtgg ccgtggagct aagtgccgtg gtcagcgagt ggaaggccgg
      601 ccggtaactt ccctgtcgtc ccggctcgcg cttcgcctgg ccgggacggc gtaattcaag
      661 ccgcgtgctc gaccttgagc acgccgcggc ggatctgatc ttcctcgatc gactcgaaca
      721 gcgccttgaa attgccctcg ccgaatccgt catcgccctt gcgctggatg aactcgaaga
      781 agatcgggcc gatggcgttc gccgaaaaga tctgcaggag caccttggtg tggccgccgt
      841 cgagcacgcc ctggccgtcg atcaggattc cgttacgtcg caggcgcgtg atgtcctcgc
      901 cgtgcccagg caggcgtgtg tcgatcttct cgaaataagt atccggcggt gacggcatga
      961 actgcaatcc cgcagcgcgc agaccttcga ccgtggaata gacatcgcgc gcgccgcagg
     1021 cgatgtgctg gatgccctcg ccgcgataga tcgccagata ctcctcgatc tggccggagt
     1081 cgcccgcatc ctcgttgatg ggaatgcgga tcttgccgtc cggactggtc agtgcgcgcg
     1141 agaacaggcc cgacgcgcgc ccctcgatgt cgaagaagcg gatctggcga aaattgaaaa
     1201 gcctttcata aaagccggcc cagacattca ttcgcccacg gtgaacattg tgggtcaggt
     1261 gatcgatgta atagagccct gctcctgcgg ggcgcgggtc acgcgagccc agccattcga
     1321 actcggcgtc atagggcgat cccttcgcac cgtagcgatc gacgaaatag agcagactgc
     1381 ccccgatacc cttgatcgcg ggcacgtcca gcgtcttgtg cgcggccgtt gcgtcggcgg
     1441 cttccgcgcc gagcgcgagc gcgcgctcaa aagcccgctt tgcatcgacc acgcggaacg
     1501 ccattgacgg cgcgcagggc ccgtgagcgg cgacgaactc gaaaccgtgg ctttccggct
     1561 cttcgttcac gatgtaattg acgtcgccct gacgatagac cgtgatcttc ctggtgcggt
     1621 gacgggcgac cgccacaaag cccatcagct tgaacagcgc gtgaagcttc gcaggctcgg
     1681 ggtgcgcata ctcgacgaac tcaaatccgt cggtgcccat cggattgtcg gcgctgatgg
     1741 tagccggcgg cgcgtcatgc ggaaacggac ccatgcggat ctccctcgat tcttgcaggg
     1801 attttccgtc gaatcaggcg caaagtgcat gcaaatccag ccatgttctg ctaccctatg
     1861 cacaattcat gcatcaaacg ggtcctttgc gcatgacttc cgtcgacgcc ttcgacctca
     1921 aaatgcttgc cgcgctgcag gacgacggcc ggctgaccaa ccaggaactc gccgagcttg
     1981 ttgggctctc agcttcgcaa tgctcgcggc gacgcatgcg gctggaagag gagaaagtca
     2041 tcgccggcta tcacgccgac ctctcgggtg aagcgcttgg cttcaacctg atcgccttca
     2101 tccacatcac gcttgcgacg cattcgccgg acaatgccaa aaaattccgc gcattgatca
     2161 atcgcgtcga cgacatccag gaagcctatt cgctcaccgg agacgcggac tatgtgttga
     2221 aggccgtact gcgcgacctc aggagccttt ccgacatcgt caacaatgtg ctgatgccgc
     2281 atccgagcgt ggctcatgtg cgctcctcga tcgtgcttga ccggctgaag gagagctcac
     2341 ggctgccgct gcgcgcacga tgagcgtgac gcaaatcgag ggaatttcgt cattcgaaac
     2401 gcggcgacga tctgcgatca tccgcgccgc gacaggagga gtcggccatg gcgctgcaac
     2461 ttcgaccgaa ttgcgaatat tgtgacaagg acttggcccc aaacgcgcag gacgcgcgca
     2521 tctgcagcta cgaatgcacc ttttgcgccg actgcgtcga gagcaagctc ttcaacgtct
     2581 gcccgaactg cggtggcggc tttgtgccgc ggccgattcg cccgtcgcgg gaatggcgac
     2641 ggggcgtgtc ggttgcaacg catccgcctt cggacaagcg cgtgcacttg aagtattccc
     2701 tcgacgaggt cgcggcgcgc gcagcgcgga tcaaggacat tccaccggaa gcgcggtgag
     2761 cggctgtcat tccgcaggca agatcgtgcc ttcaacctca ccgaagccga cgcggtagcc
     2821 gtcgccctgg caccagccgc gcatcgcaag cgaatcgccg tcctcgagga acgagcgctt
     2881 caccccgccg ccaaactcca ccggctcgct gccgttccag cttatctcca ggaggctgcc
     2941 gcgctggtgt ttttccggac cggagatggt gcccgacccc aggagatcgc cgacgttcat
     3001 ggcgcagccg cacgccgcgt gatgcaccag ttgctgcacc gaggaccagt acatgtattt
     3061 gaaattggtg cgacagacgg tctttgcttc attcatcccc gccgcgcgca gggaaacgtc
     3121 aagctccaga tcaaaattgt tggcactcgt ctggcgcaga tagggcagcg gcgccggatc
     3181 ctgcgtcggt ccacgcagac ggaacggctc cagcgcctcg cgcgtgacca cccacgggct
     3241 gatcgaggtg gcaaaggcct tggcctgaaa cggaccgagc ggcacatatt cccattgctg
     3301 gatgtcgcgc gcgctccagt cgttcaggat cacgaagccg aaaatcatct gctcggcctg
     3361 cttctcgctg agcatttcgc ccatcggcga tgattgcccg accacgacgc ccatctcgag
     3421 ctcgaaatcg agccgcttgc atggaccgaa gctcggcgtc tccacgttcg gcggcttgag
     3481 ctgcccgcgc ggtcgccgca ccccggtgcc cgagaccacc acggtcgatg cgcggccgtt
     3541 gtaaccaatc ggcatatgca gccagttcgg ctgcagcgcg ttgtccttgc cgcggaacat
     3601 gacgccgaca ttggtggcgt gctccttcga cgaatagaaa tcggtgtagc cggacaccgt
     3661 aatcggcaga tgcagcctga cgtcgcgcat cggcaccagc gcgcgccggc gcagctcctg
     3721 attatcgcgc agctcgggat gatcctcgcg caggagctcg ctgatccgcg cccgcgtcgt
     3781 ggtccacact tttgggccca gcgccataaa gggattgagt gtcggcgccg caaacacagc
     3841 cggttcgacg acatcgatcc ggcaatcctg cgcaagctgc cagaggtcga gcacataatt
     3901 gccgatcgct acgccaaccc gcggcgccag cccatccttg gccgagaaca cgccataagg
     3961 cagattctgg atcgggaaat cggatgacgg atcgaccgca atgaaggagc ggagcttcgg
     4021 atcgttgggg tggggcattg cgtcattctc ttttcttcac cctcccctgg agcgggaggg
     4081 taagaagatt acggcctgct tggatcgaag cgcttctcga gccccttcca gcaatcggca
     4141 tagtcgtcct gcagcgtgga agatttcgcg gcatgcgcag tgacgcgctg cggatagcgc
     4201 gtctcgaaca tgaaggcgag cgtgcccgtg agcttgacgg gcttcagctc accgttactg
     4261 gcatgatcga aggcctggcg atcggggcca tgcggcagca tgcaattgtg caaggacatg
     4321 ccgcccggca cgaatccttc gggcttggca tcatagacac cgtaaatcag ccccatgaac
     4381 tcgctcatga tgttcatgtg ataccagggt ggacggaagg tgttttccgc gaccatccag
     4441 cgctccggga agatcacgaa atcgatgttc gcagtacccg ccgtttccga cggcgaggtc
     4501 agcacggtga agatcgaggg atcgggatga tcgaagccga tcgcaccgac cggagagaac
     4561 gtgcgcaggt cgtatttgta gggcgcgtaa ttgccgtgcc aggcgaccac gtcgatc
//
LOCUS       DATLJT010000774         1725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_966584,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000774 DATLJT010000000
VERSION     DATLJT010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966584"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VM910_17560"
     CDS             complement(<1..417)
                     /locus_tag="VM910_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64369.1"
                     /translation="MNSRVSWNVEGIDPSIRERAEAAARRAGLSLNEWINSYLGDAAP
                     PNFRSPPQPSRDVSDIHQRLDSITRQIEQISRVSPRNDTTQELGVARQLNDAISRLDA
                     RLSQISNPQGARQAQERQQRQADAVERAASQVYANQF"
     gene            complement(632..1219)
                     /locus_tag="VM910_17565"
     CDS             complement(632..1219)
                     /locus_tag="VM910_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVI64370.1"
                     /translation="MALGDAILVCLTERPMTGYELAKTFDSSIGFFWKADHQQIYREL
                     TRLRDRGHVQAREVVQSGKPNKLVYTLTAEGKAALRHWAARPSSPPSIKDDMLVRLCA
                     LDAVDMEPMRADLMARLEYHRDRLARYERVLKKRFPHGAASLADTGKLLGLRLGLRHE
                     RAVVEWCEEALQTLTNDAPVTGVLPFSDSHRENTP"
     gene            1413..>1725
                     /locus_tag="VM910_17570"
     CDS             1413..>1725
                     /locus_tag="VM910_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI64371.1"
                     /translation="MTMTGLDRWYACAKSQDARGLLDLLHPEVVFESPVVHTPQRGRD
                     ITYKYLSSALKVLGGPTFAYVGEWRSQDGAVLEFVTEIDGTKINGVDIISFADDGRIK
                     HF"
BASE COUNT          310 a          535 c          557 g          323 t
ORIGIN      
        1 gaattgattg gcgtagacct ggctcgcggc gcgctcgacc gcatccgcct ggcgctgctg
       61 gcgctcttgc gcctgtcgcg cgccttgcgg gttggaaatc tgcgacagcc gcgcgtcaag
      121 ccgcgagatg gcgtcgttca attgacgggc aacgccgagc tcctgcgtgg tgtcgttgcg
      181 cggggagaca cgcgaaatct gctcgatctg gcgggtgatg gaatcgagcc gttggtggat
      241 gtcggaaaca tcgcggctcg gctggggcgg agaacggaaa ttgggcggag cagcgtcgcc
      301 gagataggaa ttgatccact cgttgagcga caggcctgcc cggcgcgcgg cggcctcggc
      361 cctctcgcgg atggacggat caatcccctc gacattccac gatacgcgcg agttcatgct
      421 tccgtccggt tccgctccgg cgcccccacc aggcgcctcc ggcctccccg cgcgtcccaa
      481 tcaaaagaca atcgaattct gcgcgggtcg tctgcctcta accctgactt ttgtgtgtca
      541 cggtaaataa cgggttaagg aatggccccg cgggcctcta aagttgcggt tttcaccgcg
      601 atgccgccgc aaagccgcaa gcgttcggcg atcagggcgt gttctcgcga tggctgtcgg
      661 agaacggcag gacgccggtc acgggagcgt cgttcgtcag cgtctgaagc gcttcctcgc
      721 accattcgac caccgcgcgt tcgtgacgaa gccccagacg cagtccgagc agcttgccgg
      781 tgtcggccag cgacgccgcg ccgtgaggaa aacgcttctt cagcacgcgc tcatagcggg
      841 cgagccgatc gcgatgatat tcgagccgcg ccatcaggtc ggcgcgcatc ggctccatgt
      901 cgacggcatc gagcgcgcag agccgcacca gcatgtcgtc cttgatcgat ggcgggctgg
      961 aagggcgcgc cgcccagtgc cgcagcgcag ccttgccctc ggccgtcagc gtatagacca
     1021 gcttgttggg cttaccggac tgcaccacct ccctggcttg tacgtggccg cggtcgcgca
     1081 gccgcgtcag ctcgcggtag atctgctggt ggtcggcctt ccagaagaac ccgatcgacg
     1141 agtcgaacgt cttggcgagc tcgtagcccg tcattggacg ttcggtcagg cagacaagga
     1201 tcgcgtcgcc aagcgccacg cagccaatcc tccgttaagt tgcttgactt tatgcacaga
     1261 gttgcatatg cgtcaatatg catacgatgt cccgccgtcc tctgccgctt tggcggcggg
     1321 acaggctggg ccgacaggcc cgactgatga agtgtgggtc tggcgaggac agcgcaagcg
     1381 caacgatcgc gcggcgatca gggagacgtc cgatgacgat gaccgggctc gacaggtggt
     1441 acgcctgcgc gaaatcacag gacgcacgcg gtttgcttga cctgttgcat ccagaggttg
     1501 tattcgaaag tccggtcgtg cacaccccgc agcgcggacg cgacatcacg tacaaatacc
     1561 tgtcgagcgc tttgaaggtg ctgggtggcc ccacattcgc ctatgtcggt gagtggcgca
     1621 gccaggacgg cgccgtgctc gagttcgtaa ccgaaattga cggcaccaaa atcaacggcg
     1681 ttgatatcat cagttttgcc gacgacgggc gtataaagca tttca
//
LOCUS       DATLJT010000775         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3954229,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000775 DATLJT010000000
VERSION     DATLJT010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3954229"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VM910_17575"
     CDS             <1..252
                     /locus_tag="VM910_17575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64372.1"
                     /translation="GIAAGGITDVTTRQYAAVLSKNMDQNIIVENRPIAGGAVAAAAV
                     QSAPPDGYTLLSVVGSQFCSVPAMGPAPYDPVKGFAPIS"
     gene            complement(669..1184)
                     /locus_tag="VM910_17580"
     CDS             complement(669..1184)
                     /locus_tag="VM910_17580"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HVI64373.1"
                     /translation="MLGLLILVRSWSMLLKTITAFTLAHSITLALATLGHAHPPGPPV
                     EAAIALSILFLAIEVARSWRGGTSFTIRHPWVVAFGFGLLHGFGFAGALAEIGLPSRD
                     IAVALAMFNVGVECGQLLFVAAVLLLVRALAILEFRPSRLVNALPAYAIGTLSAYWTI
                     DRVVAMFAVAG"
     gene            complement(1400..>2484)
                     /locus_tag="VM910_17585"
     CDS             complement(1400..>2484)
                     /locus_tag="VM910_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HVI64374.1"
                     /translation="EYAEQRAKWERLYEATQTKGDGEAHPFLSPNDEFANFERWDFGN
                     LDATAAKTKDMLEFEYARSALKNGLKLEKQLGTNPYKFGMVGSTDAHTALAAVEEDNF
                     FGKTTPQEPNPERLTTTFMENAKTRIKIMDWQVSSSGYAAVWATENTRESIWDAMQRR
                     ETYATTGSRMAVRFFGGWNFEQSDAQSRNPATAGYAKGVPMGGDLPPAPEGKKAPTFL
                     VAALKDPLGGNLDRIQIVKGWLDAKGELQEKVYDVVWSGDRKPGADGKLPSVGNTVDV
                     ANEIWTNTIGAPELLAVWSDPDFKATERAPDTYDVLWKVPAIGDMRAFPCMSSFRPAQ
                     KALASRVRSSSAMPRSSGGVSGERTA"
BASE COUNT          467 a          780 c          766 g          471 t
ORIGIN      
        1 ggtattgcgg ctggcggcat caccgacgtt accacgcggc aatacgcggc ggttctgtcg
       61 aaaaacatgg accagaacat cattgttgaa aatcgtccga tcgccggcgg cgcggtcgcc
      121 gctgcggccg ttcagagtgc acccccggac ggatacacgc tgctgtcagt ggtcggttcg
      181 cagttttgct cggtcccggc catggggcct gctccctacg atccggtgaa gggctttgct
      241 ccgatcagtt gacatttcgg cttccgactc tcctcgtcgt gcccatggac agcccagcaa
      301 gaactgtggc cgatctggcc tgctccttgg ctctcctggt gccggtaccc ccggccatct
      361 gcttgccgcc aaaattgcgc tcgccaccca tacgccgatt caaaccaaat taacttaatc
      421 gcgtccagac tttggaccgc agcggattgc gcgacttgcc aaaaacggac aaggtgtcat
      481 cggcttaggc tttaaaactg ccttaacgcg aagtcgccgg gcttcacctt ggcagcatac
      541 cacactgccg tttacggcaa cgaccaagcg aacacgccgt catgcgacca tcgaatacca
      601 attcagcgcc gtacagttga actttggccg cggcctcgtc tggctacgcc gtctgcctga
      661 cggggtgatc agcccgcgac cgcaaacatc gccacgacgc ggtcgatagt ccaataggcg
      721 ctgagcgttc cgatcgcgta ggcggggagc gcgttgacga ggcgtgatgg ccgaaactcc
      781 aagatcgcca aggcgcggac cagcagcagc accgcggcga caaaaagcag ctggccgcac
      841 tcgacaccga cgttgaacat cgcgagcgcg acggcgatgt cgcgggacgg cagaccgatc
      901 tcggcaagcg cgcccgcgaa cccgaagccg tgcaagagcc cgaacccgaa cgccacgacc
      961 cagggatgcc ggatggtgaa gctcgttccg ccacgccaag accgggcgac ctcgatggcg
     1021 aggaagagga tcgacagagc aatcgcggcc tcgaccggtg gtccaggagg atgcgcgtgg
     1081 ccgagtgtcg cgagggcgag cgtgatgctg tgagcaagcg tgaatgccgt gatggttttc
     1141 aggagcatcg accacgacct cacgaggatg agtaggccga gcacgaaaag caggtggtcg
     1201 aagccgagga ggatgtgctc gacgccgagc ttgaagtaag tgccggccgt ctcgagcgtg
     1261 cccggcgtcg caacgaccgt gaaagacgga gagctcggcg tcagccgcgc ggtctgcgtg
     1321 gtgccgtcga gcctttcgag gcgaaccagg acgtcagtca gggtggcgct gaggccgctg
     1381 atggtgatcg cggttccggt caggccgtcc tttcgcctga tactccaccg ctcgatcgag
     1441 gcattgccga cgaagaccgt acgcggctcg cgagagcctt ctgtgccggc cggaaactcg
     1501 acatgcaggg aaaggcgcgc atgtcgccga tcgccggcac cttccacagc acgtcgtagg
     1561 tgtcgggcgc gcgctctgtc gccttgaaat caggatcgct ccagaccgcg agcaattcgg
     1621 gcgcaccgat ggtattggtc cagatctcat ttgcgacgtc gacagtattg ccaacggatg
     1681 gaagtttgcc gtcggcgccg ggctttcggt cgccgctcca gacgacgtcg tagaccttct
     1741 cctgcaactc gccctttgca tccagccagc ccttcacgat ctgtatgcgg tcaaggtttc
     1801 cgccgagggg atccttcagc gcggcgacca ggaaggttgg cgccttcttc ccttcgggcg
     1861 cagggggaag gtcaccgccc atcggaacgc ccttggcata acccgccgtc gccgggttgc
     1921 ggctctgggc atcggactgc tcaaaattcc agccgccgaa aaaccgcacg gccatccgtg
     1981 agcctgtggt ggcgtaggtc tcccggcgct gcatcgcatc ccagatggat tcgcgcgtgt
     2041 tctcggtggc ccataccgcc gcataacccg acgagctgac ctgccagtcc atgatcttga
     2101 tacgggtctt ggcattttcc atgaacgtag tcgtcaagcg ttccgggttg ggctcctgcg
     2161 gagttgtctt gccgaagaaa ttgtcttctt ccacggcagc gagtgcggtg tgggcatcgg
     2221 tactgcccac catgccgaac ttgtatggat tcgtgccgag ctgcttctca agcttcaggc
     2281 cgttcttcag cgccgaacgg gcatattcga actcgagcat gtctttggtc ttcgcggcgg
     2341 tggcatccag attgccgaag tcccagcgct cgaagttcgc gaactcgtcg ttcggcgaca
     2401 agaacggatg agcttcaccg tcgcccttgg tctgggtagc ctcgtaaagc cgctcccact
     2461 tggcccgctg ctcggcatat tcct
//
LOCUS       DATLJT010000776        11550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3495521,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000776 DATLJT010000000
VERSION     DATLJT010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11550
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495521"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM910_17590"
     CDS             complement(<1..471)
                     /locus_tag="VM910_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI64375.1"
                     /translation="MSDIAITSFVRKELVDERPAPVKTTGFIGFLRTRLFNSPTNILF
                     TIVGVALIWFTVIPAVKFLLVDAVWSGSDRNACLEQNAGHPVGACWPYISAKFTQFIY
                     GFYPEPERWRVNLTFALAALLLLPLLIPRLPAKGLSAGLFFIAFPVVAFFLLHGG"
     gene            complement(468..1673)
                     /locus_tag="VM910_17595"
     CDS             complement(468..1673)
                     /locus_tag="VM910_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472997.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI64376.1"
                     /translation="MSIQPRSPPLQFGLRLKRALGGRAGWGGFALQVAFVILLAWIGF
                     EIAANARANLETQHITSGFGFLKNTAGFDVSQNLIPYSGSDSYTRVFLVGLLNTLLVS
                     VIGIFFATVIGFIVALGRLSPNWLISRVAGAYVELIRNLPLLFQILFWYLAVLAALPG
                     PRQSISLFGVFFLNNRGLIVPTPIGEPGLLPFTISVVLAILVSFALRAYARAQLFGKG
                     RSITIWPYVIGLLIGLPLVSGLVFGAPFVLEWPVLRGFNFAGGSRVIPEFVALLVALS
                     TYTAAFIAEIVRAGILSVHRGQMEAGLSLGLKRGSVLRQIVVPQALRVILPPLTNQYL
                     NLTKNSSLAVAIGYPDLVSVFAGTTLSQTGQAIEIIGITMGVYLLISLVTSAIMSFYG
                     WRLSRSLGT"
     gene            complement(1701..2717)
                     /locus_tag="VM910_17600"
     CDS             complement(1701..2717)
                     /locus_tag="VM910_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI64377.1"
                     /translation="MKRVSLIFTLTLATGLLSQTAQAETLKTVKDRGMLSCGVSQGLP
                     GFSSPDDKGNWTGLDVDICRGIAAAVFDDPTKIKFVPLSAKDRFTALQSGEIDVLSRN
                     TTWTLSRDTSLGANFTGVTYYDGQGFLVKKSLNVNSALELNSASVCVQTGTTTEQNLA
                     DYFKGNKMKYEVIAFASADETVKAYESGRCDVFTSDVSQLYAERLKLSNPADHVVLPE
                     VISKEPLGPMVRHGDDQWFDIVKWVLFAMVNAEELGVTQKNVDEMSKSDKPDLKRAFG
                     TDGNLGEQLGLTKDWFYRIIKAVGNYGESFDRNVGSGSKLQITRGLNQLWNKGGIQYA
                     PPIR"
     gene            3045..4238
                     /gene="metC"
                     /locus_tag="VM910_17605"
     CDS             3045..4238
                     /gene="metC"
                     /locus_tag="VM910_17605"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HVI64378.1"
                     /translation="MDSSHSPTAANPLKAETRLVTAGRDTKAQKGFVNPPVVHGSTVL
                     YPTVEDLHAHRGEFQYGRHGTPTTRALQDVLMALEGPQCAGVGLAPSGLAAVSTTLLS
                     VLKAGDHLLVCDNVYRPSRNFCNGLLARYGVETTYFDPLIGSDIEKLFKPNTRAILVE
                     APGSQSFEMPDVPAIAGVAHARDVLVVDDNTWATPLYHRSLDQGVDISMQAATKYIGG
                     HSDIMFGIISANARAWPLIAETIRLLGVCAGPDDVFLALRGTRTLAVRLAQHYRAGLD
                     MARWLADRPEVVRVLHPALESDPGHAIWKRDFTGASGLFSIVLKPKPQAAVDAMLDSL
                     KLFGMGFSWGGFESLAIPFDCSAYRTATKWAPGGPTLRLHIGLENIEDLKADLDRGFA
                     VFNAA"
     gene            4364..6058
                     /locus_tag="VM910_17610"
     CDS             4364..6058
                     /locus_tag="VM910_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HVI64379.1"
                     /translation="MGSMVLLDLMGGVALLLWGLHMVHSGVLRAFGPDLRLWLGKALN
                     NRLSAFAAGIGLTALLQSSTATALMTSSFAADGLVGLVPALAIMLGANVGTTLIVQVL
                     SFNITAVAPVLFVLGLVAFRSGPRSRLKDLGRVSIGLGLMLLSLHILLDTLAPAENAP
                     GVRVLMGAMTGDPVLCVVLAAVITWAVHSSVASVLLIMSLAYAHFITPAAALALVLGA
                     NIGSAINPLIEGARRDNPASYRLPLGNLFNRLLGAALFLPFLGPIAVHLQSWQPDLAK
                     ATAEFHILFNIFMAAIFIGVLDSLARALKWLLPDRVRESDPARARYLHESALETPSLA
                     LADAARETLRMGDMVETMLRNVMTAMMTNDRALVDQVSSMDNAVDSLDEAIKLYVTKL
                     TRGSLDDREGKRAMEIVSFAINLEHIGDIIDKNLSELATKKIKRRFQFSAEGVEELSA
                     FHKRIMDSLRIAFGVFMSGDVNEARWLLAEKAALRNTELAATERHLDRLREGRPETLE
                     TTSLHLDVLRDLRRIHSHICSVAYPVLDAAGELAALRGKDNDLASLSAPATEPSSR"
     gene            complement(6064..6381)
                     /locus_tag="VM910_17615"
     CDS             complement(6064..6381)
                     /locus_tag="VM910_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HVI64380.1"
                     /translation="MTDLINYLHDVFIIKFDGWVVLGFVAQGFFTMRFVVQWIASERA
                     RASVIPVAFWFFSIGGGVLLLIYALYRRDPVFIAGQALGLVVYIRNLYFIILTGRQSS
                     AKD"
     gene            complement(6374..7132)
                     /locus_tag="VM910_17620"
     CDS             complement(6374..7132)
                     /locus_tag="VM910_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI64381.1"
                     /translation="MSVHLPVSESDVAVSIVVPVRNEADNIAPLIAEIAAALDGRWAY
                     EIIYVNDGSTDATAERLQVVMKQCKNLRQLRHATSTGQSAAIRSGVRAARGAIVATLD
                     GDGQNNPAFLPELISTLEKGGKRVGLVAGQRVGRKDTGFKKFQSRAANAIRKAILHDG
                     TRDTGCGLKAFRRDVFLMMPYFDGLHRFLPALMRREGFDIAYVDVIDRPRHAGVSNYG
                     FFDRLWIGILDLAGVWWLIRRKKPTPVATEVLHD"
     gene            complement(7224..8006)
                     /locus_tag="VM910_17625"
     CDS             complement(7224..8006)
                     /locus_tag="VM910_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVI64382.1"
                     /translation="MTGVAESRTYAAQLSALLRESCTQLVRAPSHSQRKEGARKLARH
                     ALVLSAILGACVIALMVFLDAQEIVMMPPRGTPGLWPVRILTDFGKDTYVLWLLATVL
                     IVIALGSPALGATMRARWLHFGTRVQYLFFAVLLPVLVTEGVKWSVGRGRPFVGGKPN
                     PFNFEPFAGSEAYYSFPSAHAVTGFALAFAVGAVWPRARPWMLFYALLIAASRLVLLA
                     HHPSDVVAGALIGVLGAMAVRYWFAARQIGFAIHGDGKIVPL"
     gene            complement(8008..9762)
                     /locus_tag="VM910_17630"
     CDS             complement(8008..9762)
                     /locus_tag="VM910_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVI64383.1"
                     /translation="MVDTYARPRFGASPEPKNDPKHLVNPGSRLVWIIDFVSGSHLGA
                     TVFLLVCCALLFLPGFFAIPPVDRDEARFAQATKQMVETGDFVDIRFQDDVRYKKPVG
                     IYWMQAAAVETATALGLPGAQLRIWVYRIPSLIGAVGTVLLTYWTALAFVSRRGAVLA
                     ALIMGASVLLGVEARLAKTDAMLLLTVTAAMGALGRTYLSWQHGEDPERPPWSWPAIF
                     WTALAAGILIKGPLILMFAGLAIVMLAILDRGATWLWRLRPVWGLMWMLVLVLPWFIA
                     IYWRAGDTFFADSVGGDMLSKLAAQESHGAPPGLYLLLFWITFWPGAPLAAMAVPAVW
                     RARREPGAQFLLAWLVPSWIVFELVLTKLPHYVLPLYPAIAILTVGALELRVLSRSWL
                     TRSAAWWFAIPTLASIVAVVGAIMLTRQPAFLAWPLLAAAMIFGLFAWWLYENNHAER
                     SLLNAVAAAVFLAVAIYGVVVPSLTTLFPSAEIARALRNVRCVGPKAVAAGFHEPSLV
                     FMTGTSTLLADGSGAADFLNQGNCRFAVIEQRSERSFVQRAEAIGLRYNVASRVDGYN
                     ISQGRSASISIFRSEGTE"
     gene            complement(9813..>11550)
                     /gene="pbpC"
                     /locus_tag="VM910_17635"
     CDS             complement(9813..>11550)
                     /gene="pbpC"
                     /locus_tag="VM910_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HVI64384.1"
                     /translation="TMQLARLMEPRRERSIYAKLRQIVRAIEIERQLDKDQILNLYLA
                     MAPFGGNLEGVRAASIAYFGKEPKRLSLSESALLVALPQSPETRRLDRYPDAARAGRN
                     RVLERMVEEHRITVEDAAQASSTPVPRLRKPMPILAPHSADQAIATMRDAPLIKLTLD
                     ANLQKVLETLARDRALALGPNISVAIIVVDNESGDALARVGSPDYFDEMRAGQVDMTR
                     ALRSPGSTLKPFIYGLAFEDGFVHPESLIDDRPIRFGAYAPENFDMTFQGTVPVKKAL
                     QLSLNVPAIALLDRVGSSRLSSRLKQAGASLVLPKDETPGLAMGLGGVGVTLQDLAML
                     YAGFPRLGTTRPLREIVGSIDDASDRDRQDSLRLMDRVAAWQVGNVLLGTPPPENGIG
                     GRIAFKTGTSYGYRDAWSVGFDGRMTIAVWVGRPDGAPVPGLVGRSAAAPILFDAFAR
                     TGKLPLPLAKPPKGVVLAASNAKLPLPIRRFRPLGELVRSGGEHALHIQFPLDDSRID
                     IDRLADSEAAVMPIKVSGGVLPMTMLVNGRSVGEIDSRRQRLIDAPEPGFARLTVIDA
                     VGAADTVVVRIQ"
BASE COUNT         2356 a         3666 c         3522 g         2006 t
ORIGIN      
        1 gcccccatgc agaaggaaga acgcaaccac cggaaaggcg atgaagaaca atccggctga
       61 taagcccttc gccggcagtc gcgggatcag cagcggaagc aacaacaggg cggcgagcgc
      121 aaaagtcaga ttgactcgcc agcgttccgg ctccggataa aatccataaa tgaattgcgt
      181 gaattttgcg ctgatatagg gccagcacgc gcccaccgga tggccggcat tctgctccag
      241 acaggcgttg cggtcgctac cgctccagac cgcatcgacc aacaggaatt tcaccgccgg
      301 aatcacggtg aaccagatca acgccacacc cacaatggtg aacaggatat tggtcggcga
      361 attgaacagc cgcgtacgca agaagccgat gaaaccggtc gtcttgaccg gtgccggtcg
      421 ctcgtcaacg agctccttgc ggacaaatga cgtgatggcg atgtcgctca tgtaccgagg
      481 ctccggctaa gccgccatcc atagaagctc atgatcgcgc tcgtaacgag cgagatcagc
      541 aggtaaacgc ccatggtgat gccgatgatc tcgatcgcct gtccggtctg gctcaacgtc
      601 gtgccggcaa aaaccgaaac caggtcggga tatccgatgg caaccgcaag cgaggagttc
      661 tttgtcagat taagatactg gttggtcaac ggcggcagga tcacccgcaa cgcctgcggc
      721 accacgatct ggcgcaacac cgagccacgc ttcaaaccca gcgacaggcc cgcctccatc
      781 tgacccctgt ggaccgacaa aatgccggcg cgcacgatct cggcgatgaa ggccgcagta
      841 taggtcgaca gcgccaccag gagcgccacg aattcgggaa tgacgcgcga gccgcccgca
      901 aaattgaagc ctctgagcac cggccattcg aggacaaaag gtgcgccgaa caccagcccg
      961 ctcacaagcg gcaacccgat caggaggcca atcacatagg gccagatcgt gatcgacctg
     1021 cccttcccga acaattgagc ccgtgcatag gcccgcaacg caaacgagac caggatcgca
     1081 agcacgaccg agatcgtaaa cggcagcagc ccgggctcgc cgatcggcgt cggaacgatc
     1141 agaccgcggt tgttgagaaa gaaaacgccg aacaacgata tgctctgccg agggccgggc
     1201 aaggctgcaa gcaccgcgag ataccagaac aggatctgaa acagcagcgg caggttgcgt
     1261 atcagttcga cataagcgcc ggccacccgc gagatcagcc agttcggcga aagccggccg
     1321 agagccacga tgaacccgat cacggttgca aagaaaatgc cgatgaccga gacaaggagc
     1381 gtattgagca gtccgaccag gaaaacgcgg gtgtagctgt ccgagcccga ataggggatc
     1441 agattctggc tgacgtcgaa tccggccgtg ttcttcagaa agccaaatcc ggaggtgatg
     1501 tgttgagttt ccagattggc gcgggcattg gctgcgatct caaacccgat ccacgccagt
     1561 agaatgacga acgcgacctg cagggcaaat ccgccccagc ccgcgcgacc gccgagcgcg
     1621 cgcttcagcc gaagcccaaa ctgcagcggt ggtgaccggg gctgtatgct cattgccgca
     1681 gccttcaggc ctggcgacag tcagcggatc ggcggcgcgt attggattcc gcccttgttc
     1741 cagagctggt tgagtcctcg ggtaatctgc agcttcgagc ccgagccgac gttgcggtcg
     1801 aaagactcgc catagttacc gaccgccttg atgatccggt agaaccaatc cttggtcagc
     1861 cctagctgtt cgccgagatt gccgtcggtg ccgaaagcgc gtttgaggtc gggtttgtcc
     1921 gatttcgaca tctcgtcgac gttcttctgg gtaacgccga gctcctcagc attaaccatc
     1981 gcaaacagca cccacttcac gatgtcgaac cactggtcgt cgccatgacg caccatcggc
     2041 cccaacggct ccttcgagat cacttcggga agcacgacat ggtcggccgg attggaaagc
     2101 tttagccgct cggcataaag ctgcgacacg tcggaggtaa agacgtcgca gcgtcccgat
     2161 tcgtaggcct tgacggtttc gtcggcactg gcgaaggcga tcacctcata cttcatcttg
     2221 ttgcccttga agtagtcggc gaggttctgc tcggtcgtcg tgccggtctg cacgcaaacg
     2281 gatgcgctgt tcagctccag cgcagaattc acgttgagcg acttcttcac cagaaacccc
     2341 tggccgtcat aataggtcac gccggtgaag ttggcgccga gcgaggtgtc gcgcgacagc
     2401 gtccaggtgg tgttgcgcga cagcacgtcg atttcgcccg attgcagcgc cgtgaaacgg
     2461 tccttggccg acagcggcac gaatttgatc ttggtcgggt cgtcgaagac ggcagccgca
     2521 atcccgcggc agatgtcgac atcgagcccg gtccagttgc ccttgtcatc gggcgacgag
     2581 aatccaggca gcccctggct caccccgcac gataacatgc cgcggtcctt gaccgtcttg
     2641 agcgtttccg cctgggcggt ctgggataag aggccggtgg cgagagtgag ggtgaaaatc
     2701 agggatacgc gcttcatgac taagcctttc cagggagtcg tcctgtgaac ttcgctgttg
     2761 caatcgcaaa acgctgatgg gccagcccta aattctccat cgcttgcgct cctcccctgg
     2821 aggaggcgca cctcagtcag gcaatggatc gaaagtcgcg gcaagcccct caacgtgcgg
     2881 cgacataata gctcggaccc atcacagaac gactggcgag ccgggtcaag gggttgacgg
     2941 ccctgttctg tgcgctgtta ttaacggtcc ctggctcaaa tccgcaaaag gccgggccgg
     3001 tttaggggcg tgcggcgaga agcaccttga acaacggcga acgcatggat tcctctcaca
     3061 gccccaccgc agccaacccg ctgaaggctg aaaccagatt ggtgacggca ggccgggaca
     3121 ccaaggcaca aaagggtttt gtcaatcctc ccgtcgtgca tggttccacc gttctctatc
     3181 ctaccgtcga ggatttgcac gcccaccgcg gcgagttcca gtacggccgc cacggcacgc
     3241 cgaccacgag agcgctgcag gacgtgctga tggcgctcga aggcccccaa tgcgcgggcg
     3301 tcgggctggc gccgtcgggg cttgcggcgg tatcgaccac cctgctttcc gtgttgaagg
     3361 cgggcgatca cctcctggtt tgcgacaacg tctatcgtcc ctcacgcaat ttctgcaacg
     3421 gactgctcgc ccgttacggt gtcgaaacca cctatttcga ccctctgatc ggtagcgaca
     3481 tcgaaaagct gttcaagccg aacacgcggg ccatactggt cgaagccccg ggctcccagt
     3541 cgttcgagat gccggatgtc cctgcgatcg cgggcgtggc gcatgcgcgc gacgtgcttg
     3601 tcgtcgacga caacacctgg gcaacgccgc tttatcatcg ctcgctcgat cagggtgtcg
     3661 atatcagcat gcaggccgcg accaagtata tcggcggcca ctcggacatc atgttcggca
     3721 tcatttcggc caatgccagg gcctggccgc tgatcgccga aacgatccgc ctgctcggtg
     3781 tgtgcgccgg tcccgacgac gtcttccttg cgctgcgcgg cactcgcacg ctcgccgtcc
     3841 gcctcgcgca gcattaccgc gcgggccttg acatggcccg ctggctcgcg gatcggcccg
     3901 aagtggtcag ggtgttgcat ccggcgctgg aaagcgaccc cgggcacgcg atctggaagc
     3961 gcgattttac gggcgcatcc ggcctgttca gcatcgtgct gaaaccaaag ccgcaagccg
     4021 ccgtcgacgc catgctggac agcctgaaac tattcggcat gggcttttcg tggggtggat
     4081 tcgagagcct ggcgatcccc ttcgattgct cagcgtaccg aacggcgacc aaatgggcgc
     4141 cgggcggtcc gacactgcga ctgcatatcg ggctcgagaa tatcgaggac ctcaaggccg
     4201 atctcgaccg cggctttgcg gtgttcaacg cggcatgaat ttacttcgat cccagttgcc
     4261 tgcggcgcgt ttcggtccgg caggaaactt ccaacaacaa aaacattgat ccgctatcga
     4321 ttggcgttag cttgaacctt tgacacaagc cgagtgttga acgatgggaa gcatggttct
     4381 tctcgatctg atgggcggcg tggcattgct gctgtggggc ctgcatatgg tccacagcgg
     4441 ggttttgcgc gctttcggcc ctgacctccg gctgtggctc ggaaaagccc tcaacaaccg
     4501 cttgagcgcc tttgccgccg gaatcggact gacagcgctg ctccagagca gcactgcgac
     4561 tgcccttatg acgagttcct tcgctgccga cggtctcgtc ggtctggtgc cggcgcttgc
     4621 gatcatgctg ggcgccaatg tcggcaccac gctgattgtt caggtgctct cgttcaatat
     4681 aacagccgtt gcgccggtcc tttttgttct gggtctggtt gccttccgca gcgggccgcg
     4741 ctcccgcctc aaggatctcg ggcgcgtctc gatcggcctg ggcctgatgc tgctgtcgct
     4801 ccatatcctg ctcgatacgc ttgcgccggc ggagaatgcg ccaggcgttc gcgtgctgat
     4861 gggcgcgatg accggcgatc ccgtgctttg cgtcgtcctt gcggcagtca tcacttgggc
     4921 cgtgcattcg agtgtcgcca gcgtgctcct cattatgtcg ctggcatatg cgcattttat
     4981 cacgccagcg gcagcactgg cgctggtctt gggagcgaac atcggcagcg ccatcaaccc
     5041 gctgatcgaa ggcgcgcgtc gcgataatcc ggcaagctat cgcctgcccc tgggcaatct
     5101 cttcaaccga ctgctcggcg cggcattgtt tctgccgttc ctcgggccga tagcggtgca
     5161 cttgcagagc tggcaaccgg atctggccaa ggctacagcc gaattccaca tcctcttcaa
     5221 catcttcatg gcggccattt ttatcggcgt gctcgacagc cttgcgcgcg ccctgaaatg
     5281 gctgctgccg gatcgcgtgc gggaaagcga cccggcgcgt gcgcgctatc tccacgagag
     5341 tgcattggag acgccctcac ttgctcttgc ggatgccgcg cgggaaacgc tccgaatggg
     5401 cgacatggtc gagaccatgc tccgtaacgt catgacagcc atgatgacca acgatcgcgc
     5461 gttggtcgac caggtctcga gcatggacaa cgccgtcgac agcctggacg aagcaatcaa
     5521 gctttacgtc accaagctga cgcgtggcag cctcgatgat cgcgagggaa agcgcgcgat
     5581 ggaaattgtc tcctttgcca tcaatctcga gcacatcggc gatatcatcg acaagaacct
     5641 gagcgagctc gcaaccaaga agatcaagcg ccggtttcag ttttcagcgg agggcgtcga
     5701 agaattgtcg gcgtttcaca agcgaatcat ggactcgctc aggatcgcgt ttggcgtgtt
     5761 catgtccggc gatgtcaatg aggcgcgctg gctgctcgca gagaaggccg cgttgcgcaa
     5821 caccgaactg gccgcaaccg agcggcacct cgatcgcttg cgggagggcc ggccggaaac
     5881 ccttgaaacg acctcgcttc acctcgacgt gttgcgcgac ctgcgtcgca ttcattcgca
     5941 tatctgttcg gtcgcttatc ccgtgctcga tgccgccgga gaactcgccg cgctccgcgg
     6001 caaagataac gatttggcga gtttgtccgc gcccgcgacc gagccctcct cccgctaacc
     6061 ggctcaatcc ttggcggacg actgccgccc cgtcaggatg atgaaataga gattgcggat
     6121 atagacgacg aggccgagcg cttgacccgc gataaagacg gggtcacggc gatagagcgc
     6181 gtagatcagc aacagcacgc caccgccgat cgagaagaac cagaatgcga cgggaatgac
     6241 gctggcgcgt gcgcgctcgg aggcgatcca ctgcaccacg aagcgcatgg tgaagaagcc
     6301 ctgcgccacg aatcccaaaa cgacccagcc gtcaaacttg atgataaaga cgtcgtgcag
     6361 atagttgatc aggtcagtca tggagaacct cggtcgcaac aggtgtcggc ttcttgcggc
     6421 ggatcagcca ccacaccccg gcgaggtcga ggattccgat ccagagccga tcgaagaagc
     6481 cgtagtttga cacgcctgcg tgccgcggcc gatcgatgac gtcgacatag gcgatgtcaa
     6541 aaccttcgcg gcgcatcaac gccggcagga agcggtgcaa cccgtcgaaa tagggcatca
     6601 tcaagaacac gtcgcgccga aaggccttca gaccacaacc ggtgtcacgg gtgccgtcat
     6661 gcaggatggc cttgcggatg gcattggccg cgcgcgactg gaatttcttg aagccggtgt
     6721 ccttgcgccc gaccctctgg cccgcgacga gaccgacgcg cttgccgcct ttttcaagcg
     6781 ttgaaatcaa ttcaggcaga aacgccggat tgttctggcc gtcgccgtcg agcgttgcaa
     6841 caattgcccc gcgcgcggcg cgcactccac ttctgatagc agccgactgt ccggtcgagg
     6901 tggcgtgccg aagctggcgc agattcttgc actgcttcat gacgacttgg agccgctcgg
     6961 cggtcgcatc cgtcgagccg tcattgacgt agatgatttc gtaagcccag cggccgtcaa
     7021 gcgcggccgc gatttccgcg atcagcggcg cgatattgtc cgcctcgttg cggacgggaa
     7081 ccacgatgga caccgcgacg tctgattcag aaacaggcaa atgaacgctc atccagtgag
     7141 tgggcccgaa agcgctcttg gggcgctttt ggcgtccgct tcttatgggt ccggggcgcc
     7201 ccgggcaacc cctttagacg gccttaaagc gggacaattt tgccgtcccc gtgaatggcg
     7261 aatccgattt gacgcgcggc gaaccaatag cgcacggcca tcgcgccgag gacaccgatc
     7321 agcgccccgg ccacgacatc gctcggatga tgagccaaca gcaccagccg gcttgccgcg
     7381 atcaggagcg catagaaaag catccagggt cgcgctcgcg gccagactgc acccacggca
     7441 aaggccaggg caaagccggt gacggcatga gcagaaggaa agctgtaata agcctcactt
     7501 cccgcgaacg gctcaaagtt gaagggattg ggtttgcccc cgacgaacgg ccgtccgcgg
     7561 cccaccgacc atttgacacc ttcggtcacg aggacgggta agagcactgc gaaaaacagg
     7621 tactgcaccc gggtcccgaa atgcagccag cgggcgcgca tcgtcgcccc gagcgccggg
     7681 cttcccagtg caatgacgat cagtacggtg gccaacagcc acaagacata ggtgtccttg
     7741 ccgaaatcgg tcaggatgcg aaccggccag aggccgggcg tgccgcgcgg cggcatcatg
     7801 acgatctctt gcgcgtcgag gaagaccatc agcgcgatca cgcaggcgcc caggattgcg
     7861 cttagcacga gcgcgtggcg cgccagcttt cgcgcgccct ccttgcgctg cgaatgcgat
     7921 ggcgcgcgta cgagctgggt gcaggactca cgcagcaaag cggatagctg cgcagcgtag
     7981 gtcctggatt ccgcgactcc agtcatctta ctcggtgcct tcggaacgga agatcgagat
     8041 cgaagccgag cgcccctgcg agatgttgta gccgtcgacg cggctcgcca cgttgtagcg
     8101 cagtccgatg gcctcggcgc gctggacgaa tgagcgctcc gaccgctgct ctatcaccgc
     8161 aaagcggcaa ttgccctgat tgaggaaatc ggcggctccc gagccgtcgg caagcagcgt
     8221 cgaggtgccc gtcatgaaca cgaggctcgg ttcatggaaa ccggctgcca ccgcttttgg
     8281 tccgacgcac ctcacattgc gcagggcgcg cgcgatctcg gcgctcggaa acagggtggt
     8341 gagcgacggc accacgacgc cgtagatcgc aacggcaaga aacaccgccg cagccaccgc
     8401 attcaggagt gagcgttcgg cgtggttgtt ctcataaagc caccaggcga aaaggccaaa
     8461 aatcatcgcg gcggcaagca gcggccaggc caggaaagcg ggctgccttg tcagcatgat
     8521 cgcgcccacc acagcgacga tcgacgcgag cgtaggaatc gcgaaccacc aggcggcgct
     8581 tcgcgtcagc caggagcgcg acagcacgcg cagttccaac gcgccgaccg tcaggatcgc
     8641 gattgccgga tagagcggca gcacataatg cggcagcttg gtgagcacga gctcgaacac
     8701 aatccaggat ggcaccagcc aggcgagcag aaattgcgcc ccgggctccc gccgcgcccg
     8761 ccagaccgcc ggcaccgcca ttgcagcgag tggtgcgccg ggccagaacg tgatccagaa
     8821 caagagcaga taaagtcccg gcggcgcgcc gtgcgactcc tgcgcggcga gcttgctgag
     8881 catgtcgccg ccgacggaat cggcgaagaa ggtgtcgccg gcgcgccagt agatcgcgat
     8941 gaaccacggc agcaccagta ccagcatcca catcaacccc cagaccgggc gcagccgcca
     9001 cagccaggtc gcgccgcgat cgaggatcgc aagcatcacg atcgcgagcc ccgcaaacat
     9061 caggatcagc ggtcccttga taaggatgcc ggcggccaga gccgtccaga aaatcgcggg
     9121 ccagctccat ggcggacgct cggggtcctc gccgtgttgc catgacaggt aggtccgccc
     9181 cagcgccccc attgccgccg tcaccgtgag caacagcatg gcatccgttt tggcgagccg
     9241 cgcttcgaca ccaagcagta cggacgcgcc catgatcagt gctgcgagca ccgcgccacg
     9301 ccgggacaca aaggcgagcg cagtccagta ggtcaaaagc acggtgccga ctgcaccgat
     9361 cagcgatgga atccggtaga cccagatgcg cagttgcgct ccaggcagac cgagcgcagt
     9421 cgcggtctcg acggcggccg cctgcatcca gtaaatgcct accggtttct tgtaacgcac
     9481 gtcgtcctga aagcggatat cgacgaaatc gccggtttcg accatctgct tggtcgcctg
     9541 cgcgaaccgc gcctcgtcgc ggtcgacagg tggaatcgcg aaaaaacccg gcaggaacag
     9601 aagcgcgcag cacaccagga ggaagaccgt cgcgccaaga tggctcccgc tgacaaagtc
     9661 gatgatccag acaagccggc tccccggatt gaccaggtgc ttagggtcgt ttttgggttc
     9721 gggcgaagca ccaaaccggg ggcgcgcgta ggtatcgacc atgggttccg catacgccga
     9781 aaccaccgtc gcgacaaccg cttaggcctt gcctattgaa ttctgacgac aactgtgtcc
     9841 gcggcgccta cagcgtcgat cacagtcagc cgggcaaagc ccggttccgg cgcgtcaatc
     9901 aggcgctgcc ttcgactatc gatctcgccg accgatcggc cattgaccaa catagtcatc
     9961 ggcaagacgc caccggaaac cttgatcggc atgacggccg cctcgctgtc tgccaagcga
    10021 tcgatgtcaa tgcgcgagtc atcgagtggg aactgaatgt gcagagcgtg ctcgccacca
    10081 gagcgcacta gctctcccag tggacggaac cggcgtatcg gcagcggaag cttggcgttg
    10141 ctcgccgcca gcacgacgcc cttgggcggt ttcgccaggg gcagcggcag ctttccagtg
    10201 cgcgcgaagg catcgaacag gatcggcgcc gcagcgctgc ggccgaccag tcccggcacg
    10261 ggcgcgccgt cgggacgccc gacccacacc gcaattgtca tgcggccatc aaagccgacc
    10321 gaccaggcgt cgcgatagcc gtaactcgtg ccggtcttga atgcgatgcg cccgccgatg
    10381 ccattttccg ggggcggcgt tcccaacagc acgttgccga cctgccaggc cgcgacacga
    10441 tccatcagcc gcagcgagtc ctgtcgatca cgatcgctgg cgtcgtcgat gctgccgacg
    10501 atctcgcgca gcggtcgcgt ggtcccgagc cgcggaaacc ccgcatagag catagcgaga
    10561 tcctgcaggg taacgccgac gccgccgagg cccatggcaa gccccggcgt ttcgtccttc
    10621 ggcagtacca gactggcgcc ggcctgtttc aaccgcgatg acaaccggct cgatccgacc
    10681 cggtcgagca gcgcgatggc gggcacattg agcgagagct gcagcgcctt cttcacgggc
    10741 accgtgccct gaaacgtcat atcgaaattc tccggcgcgt aggcgccgaa gcgaatcggg
    10801 cgatcgtcga tcaggctttc cggatgaacg aagccgtcct cgaaggccag gccgtaaata
    10861 aacggcttca gcgtcgaacc gggcgagcgc aacgcgcgtg tcatgtccac ctgccctgcc
    10921 cgcatctcgt caaaataatc gggcgagccg acgcgcgcca gcgcgtcgcc gctctcattg
    10981 tcgaccacga tgatcgcaac cgaaatgttc gggccgagcg ccagcgcgcg atcgcgcgcg
    11041 agcgtctcca gcaccttctg caaattggcg tcgagcgtca gcttgatgag cggtgcgtcc
    11101 ctcatcgtcg cgattgcctg atcggccgaa tgcggcgcaa ggatcggcat cggctttcgc
    11161 agccgcggca ccggcgtgct gcttgcctgc gctgcgtcct ctacggtgat gcggtgctcc
    11221 tcgaccatcc gctccagcac gcgattgcgc cccgcacgcg cagcatccgg atagcgatcc
    11281 agccggcgcg tttccggcga ttgcggcaac gccaccagca gcgcggattc ggagagcgaa
    11341 agccgtttcg gctccttgcc gaaatacgca atcgatgccg cgcgaacgcc ctcgagattg
    11401 ccgccgaaag gcgccatcgc caggtagaga ttgagaatct gatccttgtc gagttgccgt
    11461 tcgatctcga tcgcgcgcac gatctgcctg agttttgcgt agatggaccg ctcgcggcgc
    11521 ggctccatga gccgtgcgag ctgcatggtg
//
LOCUS       DATLJT010000777         3496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4414006,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000777 DATLJT010000000
VERSION     DATLJT010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4414006"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..687
                     /locus_tag="VM910_17640"
     CDS             223..687
                     /locus_tag="VM910_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64385.1"
                     /translation="MASDKLSVRFATRGDFDKWLPLWDGYNAFYGRAGATALAREITE
                     RTWSRFFDAYEPVHCLVAESGGELLGLAHYLFHRSTTMIEPVCYLQDLFTAASARGKG
                     VGRALIEGVYREASLAGCPRVYWQTHETNRTARALYDKVAEHQGFIVYRKQL"
     gene            1053..1994
                     /locus_tag="VM910_17645"
     CDS             1053..1994
                     /locus_tag="VM910_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI64386.1"
                     /translation="MQIRNLGGSGLRVSAVGLGCNNFGQRVDLEGSRKVIHRALDLGI
                     TLFDTADIYAGMGGSETVLGTVLGDKRKDIVLATKYSKPMASDRTKQGASRRYIMNAV
                     EASLRRLKTDYIDLYQQHDYDELTPIDETLRALDDLIRQGKVRYIGNSNFPAWRVAEA
                     EHVARAMNVSRFISCQDEYSLVVRNIEKDLLPCAREYRLGVLPFFPLASGLLTGKYKR
                     GEAAPADTRFGKAPQLRDRYVTPRNEDIVEKLQVFAKERGHSMLELAFSWLAARPQVA
                     SVIAGATRVEQVEQNVKAIAWTLSPEEMVEIDRITKG"
     gene            complement(2040..3254)
                     /locus_tag="VM910_17650"
     CDS             complement(2040..3254)
                     /locus_tag="VM910_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVI64387.1"
                     /translation="MYHRESGRLLVTTRRALLLGGASFAAWAYLPKFARAADGRDPRL
                     IVVILRGALDGLATVAPIGDPDYVALHGSIALSADGPHAATMLDSFFALHPSMPEFAR
                     MYRENKAAVIHAVSTPYRDRSHFDGQDVLESGYAGPGRVQSGWLNRALEVLPKGERVT
                     RALAVGPTTPLVLRGAAPTVGWAPVALPQADDDTAARLVDLYRHRDPALGVALSQGLQ
                     LEKVALGDDMRPKPGVNNMRVVARGAAKVMAADDGPRIAALAFDGWDTHANEGGPVGR
                     LAQLLSGLDGALAEFESGLSGRWRDTVVVVATEFGRTVRINGTEGTDHGTGTIALLAG
                     GAVKGGRVIANWPTLKPASLYEGRDLAPTTDLRAVIKGVLSDHLGISENALASSVFPD
                     SAMAKPMRGLIG"
     gene            complement(3257..>3496)
                     /locus_tag="VM910_17655"
     CDS             complement(3257..>3496)
                     /locus_tag="VM910_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVI64388.1"
                     /translation="PNGFADTNAAWAAPEGMKLRLDIAAQVASRVGNAIDPRDLLELA
                     FADATSADTRHTIERAESRQQALALLLMSPEFQRR"
BASE COUNT          647 a         1149 c         1111 g          589 t
ORIGIN      
        1 gggcgcatgc tgagctacac gccggtgacc gaccttgtgc gtcacaccga cgatcccgcc
       61 aaggcggatt actatcgagc cctgcgtggc ggcgacgaac gcagattaat tcgcaatctg
      121 cgcgctgcgg agtgagccgt cgccaatcgg gccaaacgat ctgatcgttt ggcgcggagt
      181 ggttaaacga gagaatctcg acctccaacg cgcgaacaat ccatggcatc cgataaattg
      241 tccgtccgct tcgcgacgcg aggcgatttc gacaagtggc ttccgttgtg ggacggatat
      301 aacgcgttct acgggcgcgc gggcgcgacc gcgctggccc gcgagatcac ggagaggacc
      361 tggtcgcgct tcttcgatgc gtacgagccg gtgcattgcc tcgtcgccga aagtggcggc
      421 gaattgttgg ggctcgcgca ttacctgttc catcgcagca cgaccatgat cgagcccgtc
      481 tgctatctgc aggatctgtt cacggctgca tcggcgcgcg gcaagggtgt cggccgtgcg
      541 ttgatcgaag gggtttatcg cgaagcgagc ctcgcgggtt gcccgcgcgt ctattggcag
      601 acccacgaga ccaatcgcac cgcccgcgcc ctctacgaca aggtcgcgga acaccaggga
      661 ttcatcgtct accggaagca gctctgaggg ccggccaccc tgtggaaatc ttccccggca
      721 agggcgcacg gggcaaaaaa gtgcgcattt cgcgccggcc ttagcctgtg gataaataaa
      781 cctgtcaccg gcacgttaca aagaaaagct aggttggcgt tgcgtctgat caggcccccc
      841 gtcacagatc aagcgcccca gcggtccccg tcagtcgccg cgtctgaccg gggccgcact
      901 ttttttgggg ccttcgtttt tcacgacttt cgacagtgcg cggttgcgca gaccggagct
      961 gcagcagcgt agcaggacgg cgccttgcgg ggtgcggatg cgcgcgacac aatgcgtcat
     1021 cccgctcgtc tccccctaga ggactgaaat ccatgcaaat tcgcaatctc ggcggctcgg
     1081 gcttacgcgt ttccgcggtc ggcctcggct gcaacaattt cggccagcgg gtcgacctcg
     1141 aaggctctcg caaggtgatc caccgggcgc tcgaccttgg catcacgctt ttcgacaccg
     1201 ccgacatcta tgccggcatg ggcgggtcgg agacggtgct cggcacggtg ctcggcgaca
     1261 agcgcaagga catcgtgctg gcgacgaaat attccaagcc gatggcgagc gaccgcacca
     1321 agcagggtgc ctcgcgccgc tacatcatga acgcggtcga ggcgagcctg cggcggctga
     1381 agacggacta catcgacctt tatcagcagc atgactacga cgagttgacg ccgatcgacg
     1441 aaaccttgcg cgcgctcgac gacctgatcc gccagggcaa ggtgcgctac atcggcaatt
     1501 cgaactttcc ggcatggcgg gtcgcggagg ccgagcacgt ggcgcgcgca atgaatgtca
     1561 gccgctttat ctcgtgccag gacgaataca gtctggtggt acgcaacatc gagaaagacc
     1621 tgttgccgtg cgcacgggaa tacaggctcg gggtgttgcc gttctttccg ctggcgagcg
     1681 ggctactgac aggtaagtac aaacgcggcg aagcggcgcc ggcggacacg cggttcggca
     1741 aggcgccgca acttcgcgac cgctatgtca cgccgcgcaa cgaggacatc gtcgagaagc
     1801 tgcaggtctt tgcgaaggag cgcggacaca gcatgctcga actcgctttt tcctggctcg
     1861 cggcgcggcc gcaggtcgca agcgtcatcg ccggcgccac ccgcgtcgaa caggtcgagc
     1921 agaatgtgaa ggcgatcgca tggacgctgt cgcctgagga gatggtggag atcgatagga
     1981 ttacgaaggg gtagtatctc ttctccctct acccgctctt gcggggagaa gttaagaggt
     2041 cacccgatca gtccccgcat cggcttcgcc atcgcactgt ctgggaagac ggatgacgcc
     2101 agcgcgttct cgctgatacc gagatgatcc gacagcacgc ccttgatcac ggcacgcaga
     2161 tccgtggtcg gggcgagatc gcggccctcg tagagacttg cgggcttcag cgtcggccag
     2221 ttcgcgatca cccgtccgcc tttgacagca ccgcccgcga gcagcgcgat cgtgccggta
     2281 ccgtggtccg taccttcggt gccgttgatg cgcaccgtgc ggccgaactc ggttgcaacg
     2341 accacgacgg tatcgcgcca gcgcccggag agaccacttt cgaattctgc aagcgcgccg
     2401 tcgaggccgg acaacaattg cgccagacgg ccgacggggc cgccctcatt ggcatgagta
     2461 tcccacccat cgaacgccag cgccgcgatc cgcggtccgt cgtccgccgc catcaccttc
     2521 gcagcgcctc gcgccaccac ccgcatgttg ttgacccccg gcttcggccg catgtcgtcg
     2581 ccgagcgcga ccttttcgag ttgcagaccc tgcgacagcg caactcccag cgcggggtcg
     2641 cgatgccggt acaagtcgac aagccgcgcg gcggtatcgt cgtcggcctg cggcaacgcc
     2701 accggtgccc atccgaccgt cggtgccgcg ccgcgcagca ccaggggcgt cgtcgggccg
     2761 acggcgagcg ctctcgtcac ccgctcgccc ttcggcagaa cttcgagcgc gcgattgagc
     2821 cagcccgact gcacgcggcc cggccccgca taaccgctct ccagcacatc ctggccgtcg
     2881 aaatgcgagc ggtcgcgata aggcgtcgac accgcgtgga tcaccgccgc cttgttctcc
     2941 cgatacatgc gcgcgaattc cggcatcgac ggatgcaatg cgaagaacga gtccagcatg
     3001 gtcgcggcat gcggaccatc agcggagagc gcgatcgagc cgtgcagagc cacatagtcg
     3061 ggatcgccga tcggcgcgac ggtcgcaagc ccatcgagcg cgccgcgcag gatcacaacg
     3121 atgagcctgg ggtcgcgccc gtcggcggcg cgcgcaaatt tcggcaaata cgcccaggct
     3181 gcgaaggagg cgccaccgag caacaaggcg cgacgcgtcg tcaccagcaa gcggccgctc
     3241 tcgcgatggt acatcgtcat ctcctctgaa actccggcga catcagaagc agcgccagcg
     3301 cctgctgccg cgactccgcg cgctcgatgg tgtgccgcgt atcggccgac gtcgcatcgg
     3361 caaacgcaag ctcgagcaag tcgcgcggat cgatcgcgtt gccgacgcgg gaggcgacct
     3421 gcgccgcgat gtccagtctc aacttcatgc cctcgggcgc agcccatgcc gcgttggtgt
     3481 cggcaaagcc gttcgg
//
LOCUS       DATLJT010000778         1705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1426931,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000778 DATLJT010000000
VERSION     DATLJT010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426931"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VM910_17660"
     CDS             <1..1101
                     /locus_tag="VM910_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003168745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HVI64389.1"
                     /translation="YEDVNDAERLCRDPAMRWVVGDRAITGAAASASQMGRFETKWLC
                     RPENLAALADLPRRWIDKVHQRRPPRMIVFDMDSSESPTYGEQEGSAYNGHFGCTCYH
                     PLFVFNQFGDVERCALRSGNVHSADGWRAVLEPVIARYRCIVKRLYFRGDAAFAIPEI
                     YEFLETEGVSYTIRLPANSVLQSRIGYLLKRPAGRPSHEVRRYYASFSYQAQSWDKPR
                     RVVAKVEWHPGELYPRVGFIVTTLARPAERVVAFYNQRGTAEQWIKEGKGAIKWTRLS
                     CRTFSANAVRLQLHVLAYNLGNFMRTLAMPKAAEPWSLTSLREKLIKIGAKVVSHGRY
                     VTFQMAEVAVSRQMFADMLSLIARLRAPPASA"
BASE COUNT          313 a          530 c          551 g          311 t
ORIGIN      
        1 tatgaggacg tgaacgacgc cgaaagactg tgtcgcgacc cggcgatgcg ctgggtggtc
       61 ggcgaccggg cgatcaccgg agctgccgcc tcggcgagcc agatgggccg cttcgagacg
      121 aagtggctct gtcggcccga gaaccttgcc gctcttgctg atttgccccg ccggtggatc
      181 gacaaggtgc accagcgacg accgccgagg atgatcgtgt tcgacatgga ttcgagcgag
      241 agcccaacct acggcgagca ggaaggcagt gcctacaacg gccacttcgg ctgcacctgc
      301 taccatccgc tgttcgtgtt caatcagttc ggcgatgtcg agcgatgcgc gctgagatca
      361 ggcaacgtgc acagcgctga tggctggcgt gcggtgctgg agcccgtgat cgctcgctac
      421 cggtgcatcg tgaagcgcct ctatttccgg ggcgacgcag ccttcgccat ccccgagata
      481 tacgagttcc tcgaaaccga gggcgtcagc tacacgatcc ggctgccggc caacagcgtg
      541 ttgcaaagca ggatcgggta cctgctcaag cgcccggccg ggcggccgtc gcacgaggtg
      601 cgccgctact acgccagttt cagctatcag gcgcagagct gggacaaacc gcgccgggtc
      661 gtggccaagg tcgagtggca tcccggcgag ctttacccgc gtgtcggctt tatcgtgacc
      721 accttggcgc ggcctgccga gcgggtcgtc gccttctaca accagcgcgg cacggcggag
      781 cagtggatca aagaaggcaa gggcgcgatc aagtggacgc ggctgtcatg ccggaccttc
      841 tccgccaacg ccgtgcgcct tcagctccac gtcctcgcgt acaacctcgg caacttcatg
      901 cggacgctgg caatgccgaa ggcggcggag ccttggtcgc tgaccagcct gcgcgagaag
      961 ctgatcaaga tcggcgcgaa ggttgtgagc cacggccgct acgtcacctt ccagatggcc
     1021 gaggtcgcgg tgtcgcggca gatgtttgcc gacatgctgt cgctgatcgc ccggctgcgg
     1081 gcaccgcccg cgtcggcata agcggccagc ggtccaatgc ggcaagccgc aaagggaggg
     1141 gcatgcctcg atgcaagccg gtcggcgcgt gtcagcgttc cagtgccatc ggccaccggc
     1201 ttcaatcgcc ctttgctcgc gacgacagcg atttgccgtt gctcaaggcg ttcaaaggcg
     1261 cgatcttggc cccgaaatcg ccggaatctg gcgaatgtca gtttatacca actggtttgc
     1321 ctttgtaggt gaagactatc acagatgcgg cgcgccattt agtcggcaac cgcctgtggc
     1381 ggcccgtcga gcgctgcgat ggcctggacg aagcggttca gtagagcggc ttgggcagcg
     1441 ccgtcttgcg gccggtcata tagaggcgtc gatcgtccgg atcgccgtat cggcgcagag
     1501 ctcatcagcc gcggggatcg cgcgtgctgt cgacccaaag cggtcctccc acagacaccg
     1561 ccttccaggg cgaacactgt cgcagacgcg ctcaaggcct ccaggattca tgatcgattt
     1621 cggtcattcg gaaaacgcaa cccattgatg aattgacgct agggtggttt ccgaaagtct
     1681 tgatttttac actaatgcgc agcat
//
LOCUS       DATLJT010000779         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2116324,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000779 DATLJT010000000
VERSION     DATLJT010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116324"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VM910_17665"
     CDS             <1..244
                     /locus_tag="VM910_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HVI64390.1"
                     /translation="VNFFGAKDGLVHISQLAANRVQKTSDVVKEGDKVKVKLLGFDDR
                     GKTRLSMKAVDQTNGEDLEAKQKEKSEAPPREAAGE"
     gene            387..851
                     /locus_tag="VM910_17670"
     CDS             387..851
                     /locus_tag="VM910_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64391.1"
                     /translation="MPPPLIRPARTDEYDEIARVWMESWVSTGLEEASNFLLAKLRAR
                     VPQEVEKGWSLFVADDGGTLAAMLALHLPKRYLDQLFVAPQYQGCSLGRRLLAFTRTH
                     LPDEIFLRCVRENEKAWRWYEREGFVFEQEQVEPMTGFVMKHYRWKKEDGTQ"
     gene            848..1444
                     /locus_tag="VM910_17675"
     CDS             848..1444
                     /locus_tag="VM910_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI64392.1"
                     /translation="MMQLYWSPRSRSFTALWLMEETGKPYERVLTDIATGAQKKPEYL
                     AINPMGKVPALKDGDVTLAEAAAICAYVAERYPDTKLTPPIGDPQRAKFLYWLFFASG
                     CIEPAMVQIATKLEMKPFAAGWGDAQRVFDVLDAALARGPWLLGENFSAADIAIGSAL
                     NFSVRLFKMVPSRPNFEAYIARCVARPAFQRAEKIAAG"
     gene            complement(1441..2247)
                     /gene="fabI"
                     /locus_tag="VM910_17680"
     CDS             complement(1441..2247)
                     /gene="fabI"
                     /locus_tag="VM910_17680"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HVI64393.1"
                     /translation="MQDLMKGKRGLIMGIANDHSIAWGIAKTLAAQGAELAFTYQGEA
                     LGKRVRPLAQSLHVDTVLPCDVENLESVDSVFNSLRDKWGGLDFLVHAIAFSDKSELK
                     GRYADTTRENFSRTMLISCFSFTELARRAAELMPETGGAMITLTFGGSVRVMPNYNVM
                     GVAKAALEASVRYLAADFGPRGIRVNAISAGPVRTLAGSGIGDARTMFAFQQKHSPLG
                     RGVTLDELGGSALYLLSDLSGGVTGEVHYVDSGYNVISMPRPEELKGNNH"
     gene            complement(2383..3606)
                     /gene="fabB"
                     /locus_tag="VM910_17685"
     CDS             complement(2383..3606)
                     /gene="fabB"
                     /locus_tag="VM910_17685"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HVI64394.1"
                     /translation="MRRVVITGMGIVSSIGNNTQEVLSSLHQAKSGITRADKYAELGF
                     RSQVQGAPTLDPSDIVDRRAMRFLAGGTAWNHVAMEQAIQDSGLEPPEVSNVRTGIIM
                     GSGGPSARTIVESADITRSKGPKRVGPFAVPKAMSSTASAALATWFKIKGVNYSISSA
                     CATSNHCIGNAYETIQIGKQDVIFAGGCEELDWTLSVLFDGMGAMSSKYNDTPATASR
                     PYDLGRDGFVIAGGAGVLVLEELEHAKARGARVYGEIVGYGATSDGFDMVAPSGEGAE
                     RCMRMALATVDSKVDYINPHATSTPAGDPPEIEAIRKVFGTGDNCPPISATKALTGHS
                     LGATGVQEAIYSLLMMNNGFICESAHITELDPVFADMPIVRKRIDNARIGTVLSNSFG
                     FGGTNATLVFKRLDA"
     gene            complement(3650..4171)
                     /gene="fabA"
                     /locus_tag="VM910_17690"
     CDS             complement(3650..4171)
                     /gene="fabA"
                     /locus_tag="VM910_17690"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HVI64395.1"
                     /translation="MLDRRGGYEYEDLLACGRGEMFGPGNAQLPLPPMLMFDRISEIS
                     ETGGEFGKGLVRAELDVKPDLWFFGCHFKNDPVMPGCLGLDALWQMVGFYLGWIGGEG
                     RGRALGLSELKLAGQVLPNVRKVVYNIDIKRVMRSKLVLGVADGWLSMDGEIIYRAKD
                     LKVGLFKQGAAPS"
     gene            4505..5011
                     /locus_tag="VM910_17695"
     CDS             4505..5011
                     /locus_tag="VM910_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVI64396.1"
                     /translation="MPPLGTTDNRSAPTDEATDAASPHTGRQPALTGCPWHDVNEMLQ
                     AAGLRPTRQRMALGWLLFGKGARHLTAEMLYEEATLAKVPVSLATVYNTLNQLTDAGL
                     LRQVSVDGTKTYFDTNVTAHHHFYLEHNHELVDIPDPHLVLSQVPDVPDGYEISRIDM
                     VVRLRKKR"
     gene            complement(5062..5589)
                     /locus_tag="VM910_17700"
     CDS             complement(5062..5589)
                     /locus_tag="VM910_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HVI64397.1"
                     /translation="MALGRFCAVAVLVSGWLAASVSTGHTAKDTPVTASGLPIPRYVS
                     LKSDHVNVRAGPTKDNDVAWVYTRSGLPVEITAEFENWRRVRDSEGAEGWVYHSLLSG
                     RRTAVVTMKSKDELAPLYDRADANGLVTARLQVGVVAQVRRCDQHWCHVAGEGFDGWI
                     EQQRLWGVYADEKVE"
BASE COUNT         1140 a         1855 c         1742 g         1081 t
ORIGIN      
        1 cgtgaacttc ttcggcgcca aggatggcct ggttcatatc agccagcttg cggcgaaccg
       61 ggtgcagaag accagcgatg tcgtcaagga aggcgacaag gtcaaggtca agcttttggg
      121 cttcgacgac cgcggcaaga cgcggctctc gatgaaggcg gtcgatcaga cgaacggcga
      181 ggatctcgaa gccaagcaga aggaaaagag cgaggcgccg ccgcgcgaag cggccggcga
      241 atagagcttt cgacagcaag acgaaagggc ggcttttcag ccgccctttt tattcgccgg
      301 cgcgtatcgc gttacggatt ggtcggacgt gcggcgcggg acagacatgc tgctgcgcga
      361 tatggttgcg caggaggaat ctgtcgatgc caccaccgtt gatccgcccc gcccgcaccg
      421 acgaatatga cgagatcgcg cgcgtctgga tggagagctg ggtctcgacc ggtctggaag
      481 aggcaagcaa tttcctgctg gcgaaattgc gcgcccgagt cccgcaggag gtcgaaaagg
      541 gctggagcct tttcgtcgcc gacgacggcg gcacgctcgc ggccatgctg gcgctgcatc
      601 tgccgaaacg ttacctcgac cagcttttcg tcgcgccaca atatcagggc tgctctctcg
      661 ggcgacggct gctcgccttc acccgaacgc atttgcctga cgagatattc ctgcgctgcg
      721 tgcgtgagaa cgagaaggcc tggcgctggt acgagcgcga aggctttgtt ttcgagcagg
      781 aacaggtcga gcccatgacg ggcttcgtca tgaaacacta ccgctggaag aaagaggatg
      841 gcacacaatg atgcagctct actggtcgcc gcgttcgcgc tcattcaccg cgctctggct
      901 gatggaagag acgggcaagc cctatgagcg cgtgttgacc gacatcgcga ccggcgcaca
      961 aaagaaaccc gaatatctcg cgatcaatcc gatgggcaag gtgccggcgc tgaaggacgg
     1021 cgacgtgacg ttggccgagg ccgctgcgat ctgcgcctat gttgccgagc gctatcccga
     1081 cacgaaactg acgccgccca tcggcgatcc ccagcgcgca aaattcctct attggctgtt
     1141 cttcgcctca ggatgcatcg agcccgcgat ggtgcagatc gcgaccaagc tcgagatgaa
     1201 gcctttcgcc gccggctggg gcgacgcgca gcgtgtgttc gacgtgcttg acgcggcgct
     1261 cgccaggggt ccgtggcttc tcggcgagaa tttttcggcc gccgacatcg cgatcggctc
     1321 ggccctgaat ttctcggtgc ggctgttcaa gatggtgccc tcgcgcccga atttcgaggc
     1381 ctatatcgcg cgctgcgtcg cacggccggc gttccagcgc gccgagaaaa tcgcggcggg
     1441 ctaatgatta ttgcccttca gctcttccgg acgtggcatc gagatgacgt tgtagccact
     1501 atcgacgtaa tgcacctctc cggtgacacc gcccgagaga tccgacagga gatatagcgc
     1561 cgagccgccg agttcatcca gcgtgacgcc gcggccgagc ggcgaatgct tctgctgaaa
     1621 ggcaaacatg gtgcgcgcgt cgccgatgcc ggagcccgca agcgtccgca cgggaccggc
     1681 cgagatcgcg ttgacacgga tgccgcgcgg gccgaaatca gcggcgaggt agcgcaccga
     1741 ggcttccagc gcggctttgg caacgcccat gacattataa ttcggcatca cccgcaccga
     1801 tccgccgaag gtcagcgtga tcatcgcgcc gcccgtttcc ggcatcagct cggccgcgcg
     1861 cctggcaagc tcggtaaatg aaaagcacga aatcagcatg gtgcgcgaaa aattctcgcg
     1921 cgtggtgtcg gcgtagcgtc ccttcagctc gcttttgtcg gagaaggcga tggcatggac
     1981 caaaaaatcg agcccgcccc atttgtcgcg caagctgttg aagacggaat caacgctctc
     2041 gagattctcg acgtcgcagg gcagcaccgt gtcgacatga agcgattgcg ccagcggacg
     2101 cacccgcttg cccagcgcct cgccctgata ggtgaaagcg agctctgccc cctgtgctgc
     2161 cagcgtcttt gcgatccccc aggcaatgga atgatcattg gcgatgccca tgatcagtcc
     2221 gcgcttgcct ttcatcaggt cctgcattgg gcatcactcc ctgattcact atgcccgggc
     2281 cccgcgggga catccacgtc ctcttaggat tttcgtgtat caagacgtgg atggccggga
     2341 caagcccggc catgacacca tcccactcga atttccgcaa aatcacgcat ccaggcgctt
     2401 gaagaccaac gtggcgttgg tgccgccgaa gccaaaggaa ttcgacagaa ccgtgccgat
     2461 cctggcgtta tcgatacgct tgcgcacgat cggcatgtcg gcgaagacgg gatcgagctc
     2521 ggtgatgtgc gcgctttcgc agatgaaccc gttgttcatc atgagcagcg aatagatcgc
     2581 ctcctgcacg ccggtggcgc cgagcgagtg cccggtgagc gccttcgtcg ccgagatcgg
     2641 cgggcaattg tcgccggtgc cgaatacctt tcggatcgcc tcgatttcgg gcgggtcgcc
     2701 ggccggcgtc gaggtggcat gcgggttgat gtaatcgacc ttggaatcca cggttgcgag
     2761 cgccatgcgc atgcagcgtt cggcaccctc gcctgacggt gcaaccatgt cgaagccatc
     2821 ggacgtggcg ccgtagccca cgatctcgcc gtagacgcgc gcgccgcgcg cttttgcatg
     2881 ttccagctct tccagcacca atactccggc gccgcccgcg atgacaaaac cgtcgcgacc
     2941 gaggtcgtag gggcgcgagg cggtcgcggg cgtgtcatta tatttcgacg acatcgcccc
     3001 cataccgtcg aacaagaccg acagcgtcca gtcgagttcc tcgcagccgc ccgcgaagat
     3061 cacgtcctgt ttgccgatct ggatggtctc gtaggcattg ccgatgcaat ggttggaggt
     3121 ggcgcaagcc gacgagatcg aatagttgac gcccttgatc ttgaaccagg tggcaagcgc
     3181 ggcggaagca gtggacgaca tcgctttcgg caccgcaaag ggtccgaccc gctttggtcc
     3241 cttgctgcgc gtgatgtcgg cagattccac gatggtgcgt gccgacggac cgcccgagcc
     3301 catgatgatc ccggtgcgga cgttggagac ttcgggaggc tccaggccgg aatcctggat
     3361 cgcctgctcc atcgcgacgt ggttccaggc ggtgcctccg gcgagaaagc gcatcgcgcg
     3421 gcggtcgaca atatcggaag gatcaagcgt cggcgcgccc tgcacctgcg agcggaagcc
     3481 aagctctgcg tacttgtccg cacgggtgat accggacttg gcctgatgaa ggctcgacaa
     3541 cacctcctgg gtgttgtttc cgatggatga gacaatgccc atccccgtga tgacaacccg
     3601 cctcatgttc gcctcgccct gcttttgtcg cttcgtgcac cggttccgtt caagaaggcg
     3661 cagcgccttg cttgaacaag ccgaccttca ggtccttggc gcgatagata atctcgccgt
     3721 ccatggaaag ccacccgtcg gcgacgccta acaccagctt tgagcgcatt acgcgcttga
     3781 tgtcgatgtt gtaaacaacc ttgcggacgt tgggcagcac ctgaccggcg agtttcaatt
     3841 cgcttaatcc cagcgcgcgg ccgcgaccct cgccaccgat ccagccgagg taaaaaccga
     3901 ccatctgcca cagcgcatca agtccgagac agcctggcat gaccggatcg ttcttgaaat
     3961 ggcaaccgaa gaaccagagg tccggcttga cgtcgagctc cgcacgcacc aaacccttgc
     4021 cgaattcgcc gccggtttcg ctgatttcgc tgatacggtc gaacatcagc atcggcggca
     4081 gcggtaattg tgcgttgccc gggccgaaca tctctccccg gccgcaagcc agcaaatcct
     4141 cgtactcata accgccgcgc cgatccagca tgccacgtag cctctttcac aatctccgct
     4201 agagctttct ttaaagcgaa acggacccgt ttgcgcggga agcgcgccgg attaccgcaa
     4261 aaatcggcgc gaattcctca gcccggacac ctcgcggctg gtgatcgcca tgccaaaatc
     4321 ggttagccgg gtccgcgggc tctctatcat agggaccgcg ggggtcaaag cgttccagcc
     4381 ggccgaatcg acgcatttta tgatcgtttc gcagggcaga tcagacctgc tccagttgcg
     4441 agaaacttgc atctgcgcaa tttccttcat atatttcgcg gcgatagtcc gggaaatgag
     4501 cgccgtgccc cctttgggaa caactgacaa tcgttcggct ccgaccgacg aagcgactga
     4561 tgccgcgtcc cctcacacgg gacggcagcc cgcattgacc ggctgcccct ggcacgacgt
     4621 caacgaaatg ttgcaggctg ccggcctccg ccccactcgt cagcgcatgg ctctgggctg
     4681 gctgttgttc ggcaagggtg cccgccatct caccgccgaa atgctttacg aggaggcgac
     4741 gctcgccaag gtgccggtgt cgcttgccac cgtctacaac accctcaatc agctcaccga
     4801 tgccggcctg ttgcgccagg tttcggtcga cggcaccaag acctatttcg acaccaacgt
     4861 caccgcccat caccatttct atctcgagca caatcacgag ctcgtcgata ttcccgatcc
     4921 gcatctcgtg ctgtcacaag tgccggacgt gcctgacggc tacgagattt cgcgcatcga
     4981 catggtggtg cggctgcgca agaagcgctg accgctgtcg tcccggccaa gcgaagcgcg
     5041 aaccgatacg acgcgagaaa ttcactccac cttctcgtcg gcgtagacgc cccacagccg
     5101 ctgctgctcg atccagccgt caaagccctc gcccgcgaca tggcaccaat gctggtcgca
     5161 tctcctgacc tgggcgacga cgccgacttg caggcgcgcg gtaacgagac catttgcatc
     5221 ggcgcgatcg tagagcggcg cgagttcatc cttgctcttc atggtgacga ccgcggtgcg
     5281 gcggcccgac agcagcgagt gatagaccca gccttcggcg ccttcggaat cccgcacgcg
     5341 ccgccagttt tcgaactcgg cggtgatttc cacgggtagc cccgagcggg tgtagaccca
     5401 ggcaacatca ttgtccttgg tcggaccggc cctgacattc acgtgatcgg atttcaggct
     5461 gacgtaacgc gggatcggca ggccgcttgc ggtgacgggc gtgtccttgg cggtgtgccc
     5521 ggtcgagacg gaggcggcaa gccaaccact cacgagcacc gcgacagcac aaaaacgccc
     5581 caacgccatc aacaagtctc cccgacgggc acgtctcaac tcttgaaacc ttcgagctga
     5641 actacgaaac ccgcgcctga tccccatatc ccctgccccg gtcatccctg gccttcgcaa
     5701 tggccgggat ttgccggagt tcttgtcttg gcccggcctt ctgctagaga ggacggaacg
     5761 ccagcaacca agacggcccg aagcctctgg cgaatcctga aagggaagca acgggaac
//
LOCUS       DATLJT010000780         3890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1887248,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000780 DATLJT010000000
VERSION     DATLJT010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887248"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VM910_17705"
     CDS             complement(<1..294)
                     /locus_tag="VM910_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HVI64398.1"
                     /translation="MSRRHSAEKREVLPDPKFGNIVITKFMNSVMYAGKKSVAEGIVY
                     GALGIIETKTKQNPLTIFEQALENVMPTIEVRSRRVGGATYQVPVEVRAVRRQA"
     gene            complement(409..780)
                     /gene="rpsL"
                     /locus_tag="VM910_17710"
     CDS             complement(409..780)
                     /gene="rpsL"
                     /locus_tag="VM910_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HVI64399.1"
                     /translation="MPTINQLIASPREVQKSRKKVPALQQSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHILRGVL
                     DTQGVKNRKQRRSKYGAKRPK"
     gene            complement(1395..2570)
                     /locus_tag="VM910_17715"
     CDS             complement(1395..2570)
                     /locus_tag="VM910_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64400.1"
                     /translation="MQRYFSGLSRNTFLLALSSLFSDISTEMLYPVLPVFLTDVLHAN
                     GSIVGLVDGFAQATQNIVQGFSGAIADRLQRRKPVALVGYLLAAVAKPLMGIATIWEG
                     LFAARLIDRLGAGIRSAPRDALVASSVAERDRGRAFGLEGLGDNAGAFLGPLVALLLL
                     YAFHLDLRTIFYLAVVPGLLAFLMVLLVSERHVDVAAKAKIDVNLRRLPKRYWKYLLA
                     TALFGLGNSSNAFLILQTRDVGVSLGVTILIYAAFNLMAALISYPAGALSDRRGRRSL
                     LLASFAVFFMTYIGFALTRNVVAIGVLFALYGLYQGVFRAVGKALASDLVPRDLRASG
                     VGWYSTTVGLLQLLASVIAGLLWDRVGHASVFFFGALFALVGAVGLLALVPKTISES"
     gene            complement(2595..3818)
                     /gene="chrA"
                     /locus_tag="VM910_17720"
     CDS             complement(2595..3818)
                     /gene="chrA"
                     /locus_tag="VM910_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HVI64401.1"
                     /translation="MTSPALPLQRAGKGTALEVLLVFLRLGLSSFGGPIAHIGYFRDE
                     FVTRRRWLDEQSYADLVALCQFLPGPASSQAGFSIGLMRAGYLGGLAAWTGFTLPSAF
                     LLLLFAYGVGSLGGPLGAGLLHGLKLVAVAIVAQAVWGMARSLCPDRERASIAAVAAL
                     LILFSTSSVVQLAAIVLGGVAGLLLCRDAPPAAGGHVAMPVSRQAGIIALVAFFVLLL
                     GALLLQGRLSLQGFDLFAAFYRSGALVFGGGHVVLPLLREAFVAPGWVSDDAFLAGYG
                     AAQAVPGPLFTFAAYLGAVSKLQPSGLAGAAIGLIGIFLPGILVLVGMLPFWDAFRVR
                     AGAQAAMRGVNAAVVGILGAALYNPVWTTSVKGPADLAIALVGFVLLVAWRAPPLVVV
                     IVSAIGGIALALAHA"
BASE COUNT          827 a         1292 c         1167 g          604 t
ORIGIN      
        1 ggcttgccgg cgcaccgcgc gcacctccac cggcacctga taggtagcac cgccgacacg
       61 gcgggagcgc acttcgatgg tcggcatcac gttttcgagc gcctgctcga agatcgtcag
      121 cggattctgc ttggtcttgg tctcgatgat ccccagcgcg ccgtagacga tgccctcggc
      181 gaccgatttc ttcccggcat acatcaccga gttcatgaac ttcgtgatga cgatgttccc
      241 gaacttcgga tccgggagca cttcacgctt ttcggcagaa tggcgacgcg acatctgttt
      301 tctttcctcg tcatgcccgg gctcgtcccg gccatccacg atttaactct ttgctccaaa
      361 tcccgggtct cgctgggcgt gcccgggatg accttcgctg tgccccggtt acttcggacg
      421 cttggcgccg tacttggagc ggcgctgctt gcggttcttg acgccctggg tgtccagcac
      481 gccgcggaga atgtggtagc gcacgccggg cagatccttg acgcggccgc cgcggatcat
      541 gaccaccgag tgctcctgaa ggttatggcc ttcgccgggg atatagccga tcacctcaaa
      601 accgttggtc aggcgcacct tggccacttt ccgcagcgcc gagttcggct tcttcggggt
      661 ggtggtatag acgcgcgtgc acacgccgcg cttctgcggc gactgctgca gcgccggcac
      721 cttcttgcgc gacttctgta cctcgcgcgg acttgcgatc agttggttga tcgtcggcat
      781 cttggccttc accctctaaa cgttcacgca aagccgaggt cggccctgca aattcctttt
      841 cggggtaccc cgccccgccg ggccgccgcg cgcgaacgca aaaaacgatc gcgcaaagcg
      901 aaattgcgcc aaccgctcca tcgctgagcg gaaagcgctt cgacgccaca gaggaccgcg
      961 gaaaacgacc gtcagcgttc gctgttcggt ctgaaaccga ggtcatgcat ccgagttcga
     1021 tctcaagagg acgcgctcga gagaactaaa atcgtcctgg cattgcctag ctatgatcga
     1081 cagcgtttga gcggcattcg tcgaggttgg tgcccatctc gacctccaaa cagaggcgat
     1141 ttgggtgttc cgttccgacg ttggcgttgc tcaccgcctg tcgttaagtg ggcgcctttt
     1201 atagagggcg cataaccaag tcaagccgaa acgatcctgc aagaggacag acgaaaggct
     1261 tctgccgctt gcggaatcta cccgttcgcg agcatccgtc ggcgcatccg gcagcccgcc
     1321 ccgggacaat ttttgggccg ccgcggccga atttacccgg atataatttg cgtcacccga
     1381 gcctgcaggc cgattcagct ttcgctgata gtcttgggca ccagcgccaa caggccgacg
     1441 gcgccgacca gagcgaacaa cgcgccgaag aagaagaccg aggcgtggcc cacgcgatcc
     1501 cacaacagcc cagcgatcac gctggccaga agctgcagca ggcccaccgt ggtgctgtac
     1561 caaccaaccc cgcttgcccg cagatccctc ggcacgagat ccgacgccag cgccttgccc
     1621 actgcgcgga agacgccctg gtacaatccg tagagcgcaa acagcacgcc gatcgcgacg
     1681 acatttcgcg tcaacgcaaa gcctatatag gtcatgaaaa acacagcgaa agaggccagc
     1741 agtaggctgc gtcgtccgcg gcggtccgac agcgcgcccg ccgggtagga gatcagcgcg
     1801 gccatgagat tgaaagcggc gtagatcagg atcgtgaccc ccagcgacac gccgacgtcc
     1861 cgggtttgca gaatcaggaa ggcgttgctg gaattgccga ggccgaacag agccgttgcc
     1921 agaagatact tccagtaccg ttttggaagc cggcgcagat tgacgtcgat tttcgccttt
     1981 gccgcaacgt caacgtgcct ctcgctcacc agtagcacca tcaggaaggc gagcagtccg
     2041 ggaacgaccg caaggtaaaa gatggtccgc aggtccagat gaaaggcgta aagcaagagc
     2101 agcgccacca gcggtccgag gaaagcgccg gcattgtcgc caagaccctc cagcccgaac
     2161 gccctgcccc ggtctcgctc ggcgactgaa gatgccacga gggcatcgcg tggtgcggag
     2221 cggataccgg cgccgagccg atcgatcagt ctcgcggcaa acaggccctc ccaaatcgtc
     2281 gcgatcccca tcagcggctt ggcgaccgcg gccaagagat aaccgaccag cgcgacgggc
     2341 ttgcgccgtt gcagccgatc cgcaatcgcc ccggaaaatc cctgcacgat attttgcgtg
     2401 gcctgggcaa atccgtcgac caggcccacg atgctgccgt ttgcatgcag cacgtcggtc
     2461 agaaaaaccg gcaggaccgg atagagcatc tcggtcgaga tgtcggaaaa caggctggag
     2521 agcgcgagca gaaaggtgtt gcgcgacagc cccgaaaaat agcgctgcat cgaggcgacc
     2581 acgaccgaaa ccctttatgc gtgcgcgagc gcgagtgcga tgccgccgat cgcactcacg
     2641 atcacgacca ccagcggcgg cgcgcgccag gcgacaagca gcacgaaacc gaccagagcg
     2701 atcgcgagat ccgcaggccc cttcaccgac gtcgtccaca ccggattgta aagcgcggcg
     2761 ccgagaatcc cgacgaccgc ggcattgacg ccgcgcatcg ctgcctgcgc gccggcccgc
     2821 acgcgaaacg cgtcccagaa cggcagcatg ccgacgagaa cgaggatgcc tggaaggaag
     2881 atgccgatca gcccgatcgc cgcgcccgca agcccgctgg gctgaagctt gctgaccgcg
     2941 ccaagatagg ctgcgaaggt aaagagcggc ccgggtaccg cttgcgccgc gccatagcct
     3001 gcgagaaacg cgtcatcgct gacccagccc ggtgcaacga aggcttcccg caacaatggc
     3061 agcaccacat ggccgccgcc gaacaccagc gcgcccgagc gatagaacgc ggcgaacagg
     3121 tcgaaaccct gcagcgacag ccgcccctgc aacagcagcg cgcccagcaa cagcacaaaa
     3181 aaggcaacca gcgcgatgat tcccgcttga cgcgataccg gcatcgcaac gtgcccgccg
     3241 gccgccggcg gcgcatcgcg gcacaggagg agccccgcga caccgccaag cacgatcgcg
     3301 gcaagttgca cgactgacga ggtgctgaac agaatgagga gcgccgccac ggccgcgatc
     3361 gaagcccgct cacggtcggg acataacgag cgcgccatgc cccagaccgc ctgggccaca
     3421 atcgcaaccg caaccagttt caagccgtga agcagtccgg cacctagcgg gccaccgagc
     3481 gaaccgacgc cataggcaaa aagaagcagc agaaaagccg acggcagagt gaaaccggtc
     3541 caggccgcaa gcccgccgag atagcccgcg cgcatcaacc cgatcgaaaa acctgcctgg
     3601 ctgctcgccg gccccggcag aaactggcac agcgcgacga gatcggcata ggactgctcg
     3661 tcgagccaac gccttcgcgt gacgaactcg tcgcggaaat atccgatatg cgcgatcggc
     3721 ccgccgaaac tggacaggcc gagcctgaga aacaccagca ggacttcgag ggcggttccc
     3781 ttgccggccc gttggagcgg aagagcgggg cttgtcatgc aatttctttg tcgtgccgat
     3841 cctgcaggca ttggggcaat gccgcctcga cggcttcagt cctcgttctc
//
LOCUS       DATLJT010000781         7174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2806786,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000781 DATLJT010000000
VERSION     DATLJT010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806786"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VM910_17725"
     CDS             <1..185
                     /locus_tag="VM910_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64402.1"
                     /translation="VTTLPYARSYALNMGTARRYAPCKIFPLIRFEGAERVQQIFKAV
                     DNLRELRPALHKAAQS"
     gene            365..1087
                     /gene="phoU"
                     /locus_tag="VM910_17730"
     CDS             365..1087
                     /gene="phoU"
                     /locus_tag="VM910_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVI64403.1"
                     /translation="MVHARHTTKVFDSDLQELARAIAEMGGLAERQIAEAIEALTTRD
                     SGPAQRIVAADATIDAMQRNIEERAVETIARRQPVAGDLREIVGILRIANEIERIGDL
                     AKNIGKRIIAINGEDASRGSMRGVSQMAALMLAQLRDVLDSFACRDVAKAVEVWTRDQ
                     DIDRLCTSLSQELLAYMMEKSVAMSFGAHLMFCAKNLERMGDHATNIAEAVHYMVKGE
                     TLLRERPKADFTSMLTVASGRT"
     gene            complement(2616..2921)
                     /locus_tag="VM910_17735"
                     /pseudo
     CDS             complement(2616..2921)
                     /locus_tag="VM910_17735"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            3231..3908
                     /locus_tag="VM910_17740"
     CDS             3231..3908
                     /locus_tag="VM910_17740"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI64404.1"
                     /translation="MNNVTRGALRSSLFGILALAALLFIPAGTLDYWQAWLFMAVFVC
                     ASAAIGVYLAIRDPKLLERRMRVGPRAEKEPAQKIIMVFATLGFIVMLVFPALDRRFG
                     WSAVPGSVSVLGDALIALGFLFTFFVLRENTYSASTIQIAEGQTVISTGPYALVRHPM
                     YAGALVMLIGIPLALGSWWGLFALLFILPVLIWRLLDEERFLRQNLAGYADYQAKVKY
                     RLLPFIW"
     gene            4078..5295
                     /locus_tag="VM910_17745"
     CDS             4078..5295
                     /locus_tag="VM910_17745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI64405.1"
                     /translation="MKPNLVPLHAWLIEAGTSDVAAEALFDGFCGRLAAAGVPIARGF
                     LSIAGLHPLRRAYSLTWHDGRIAEVTDFGHATMATAVWQTSPFRHMLETKTRRLHRRL
                     LGDDAVLDFEVLREFRDAGMSEWLALMYGFGWSVALPATNQFGVVLSWTTSAAVGRGN
                     GWSAMELAAIEELSGTLALAIKGNSAPVAAHDLLVAYLGGDAADRVSAGQVQRGSVSR
                     IAAVILCADVRGFTNFAEETAPEEVTRRLNGIFDCLGDPMRAAGGEILKFLGDGVLAV
                     FLPPDGANVAIVTPAALAAAREILARVEALNATETAAGNPALSLDIALHAGEVTYGNI
                     GTADRLDFTVIGPAVNEAARLEGLCKELGMPILISKSFAQAAEGVRDQLLSVGRHPLR
                     GVREPQEVFTICT"
     gene            5547..5783
                     /locus_tag="VM910_17750"
     CDS             5547..5783
                     /locus_tag="VM910_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64406.1"
                     /translation="MVAQEPERLLFADEEAADAVGAVVDAGGVAAHTVVASTNKSLAQ
                     MNKSPDVGKATKLYRPEGDVGEAKNQHTSPTAAE"
     gene            5880..6032
                     /locus_tag="VM910_17755"
     CDS             5880..6032
                     /locus_tag="VM910_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64407.1"
                     /translation="MTSDDSLSLILRRLDEIERKLDHMLEELRTLNTPTAIGYSVRRG
                     NLPKKD"
     gene            6202..7173
                     /locus_tag="VM910_17760"
     CDS             6202..7173
                     /locus_tag="VM910_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64408.1"
                     /translation="MLTRRSLLAVSAAWLFGAADAAAQDWPQRPVRVIVPFGPGGGAD
                     IIGRIVAQSLQEKLGQPVVIENRPGAGGTLGNEAVARADKDGYTLGIMTAGQIIAAVM
                     SKSLRYDTLTAFDPVSQVATAGLIIVTRPDFPADDVKQLIAAAKANPGKISFASPGFG
                     ATQHFTGELFRQTAGVNILHVPFRTSPEAIAAVLGKQVDVLFDTVSAVLGQVQSGQLK
                     ALAVTGRDRFPAVPDVPAAIESGVLPGYDVTTWYGVFAPRDTPPAIIAKLNTIINESL
                     KEDAVQKRLTTAGVVVRGSTAEAFGEHMAREFKRWNTVREAAGIPQQ"
BASE COUNT         1322 a         2160 c         2304 g         1388 t
ORIGIN      
        1 gcgtaacgac attgccttac gcacgatcgt acgccctgaa catgggcacg gcacggcggt
       61 atgcgccgtg caaaattttc ccgctgattc gatttgaggg cgccgaaaga gtccagcaaa
      121 tttttaaggc agtggataac ttacgcgaac ttcggccggc gctgcacaag gccgcgcaat
      181 cgtagtactg caatcgatcc gcacggcgca cgaagtggat gagaagatca tgcgtcgccg
      241 ctggtcgctt gaaacgccaa cacgatcgcg cgcggccctt catcgtcatc gcgcggacat
      301 cgaaccaaga taaaaggacc tttcgcgtcg tgtgctccct acggcttgga ttggtcccac
      361 cacaatggtt cacgccagac acaccaccaa agtcttcgac tctgacctgc aggaactcgc
      421 gcgggcaatc gccgagatgg gtgggctggc cgagcggcag attgccgaag cgatcgaggc
      481 gctgacgacg cgcgacagcg gtcctgcgca gcggatcgtc gccgccgacg cgacgatcga
      541 tgccatgcag cgcaacattg aggaaagggc ggttgagact attgcccgtc ggcagccggt
      601 agccggtgac cttcgcgaga ttgtcggcat cttgcggatt gcgaatgaga tcgagcgcat
      661 aggggatctt gccaagaata tcggcaagcg gatcattgcc atcaacggtg aagacgcgtc
      721 gcgggggtcg atgcgcgggg ttagccagat ggctgctctc atgttggctc agttgcggga
      781 tgtcctcgac agcttcgcgt gccgcgatgt cgcgaaggcg gtcgaagttt ggacgaggga
      841 ccaagacatt gaccggctgt gcacgagttt atcgcaggag cttctcgctt atatgatgga
      901 aaagtccgtc gcgatgtcct tcggcgctca cctgatgttt tgtgcaaaga atcttgagcg
      961 catgggcgat cacgcgacca atatcgccga agccgttcac tacatggtga agggcgagac
     1021 gctcttgcga gagcgaccaa aagcagattt caccagcatg ctgaccgtgg cctcgggacg
     1081 gacatgacaa aaccgacccc ggtgtctatc gagaactccc tccttattga ttgcgcgcct
     1141 cgggcatctg gaaaatcaac agcggcgacg cgaacaggtt ctccacgcgt ttcaatgcga
     1201 tcgtctccat ggctcactcc gttcggaccg gcaaaacgac gggttagcgc tcgcggaacg
     1261 cgcgcaggat ttgatcgtgc aggggcttgg tgaagtatga aatgacggtg cggtcgtaag
     1321 ttttgatgaa cgcctcgacc ggcatacccg gcaccagccg tcgtgacgaa atagaacttg
     1381 ttgtgatcga tcgtgtccca tgcgccgtct tctggccgct cgaagccggt cacgtgtcca
     1441 gcctggctgt tgctctcgat ttgggcgccg gtgagattgt ggacatcggc ggacgtgttg
     1501 gccgcgttgc cgaccgccac caggtcgaaa tgcgagccgt tcaccgcctt ggtggtgaga
     1561 tcggtctcga attccattcc tgccaccttg acgccgtaca tgccgccgcc ggtgagcccg
     1621 gccttatcga tcgcaagccc ggtcgactgc ttgtcgccga catagaagta gacctcgccg
     1681 cggtcgttgc cggaaaacgt cgagccgccg ccgctgttgc cggccgccgc cgtgtcgttg
     1741 agcatcgcga ccacggtctt gtcgccggtg gacgggttgg cgagctggtt ctcgtaggcc
     1801 atgttgccga gccaggcgag ctcgtagctg ttgccgtcct gcgcgccgcc gacgatgtgc
     1861 gccatcgcgc gaccctcggc gttgttctcc tcgccgtcga ggaacaggcg gccgtcggca
     1921 ggattgaagc cgagaccggt cgccggattg tagaatgcgt tggcgtcggc caggtccgcc
     1981 gagcagaacc gactgaacga tatgccgtta cccaacgcgg cattgtggtc gacgaaggtc
     2041 tgcgtcgcgg tatcgaacag ccaggcgtcg tgcatcagat cgtggccgga gaggacctgc
     2101 agcgtcgtct tgtcgatcac ccactccgac acgaaagcgc cgttggcgcc gtgcgcgcgc
     2161 accaccccgg ccaatgcggg gttgctgttc gaaatctcat ggttcatcag aaccgtaaac
     2221 gtgccgttgc cgttgtcgaa agcgccgagg ccgtcgggaa tgccaaccat ggtatagccg
     2281 ttgagcgcct gatcccccgt ggtgatcaac gactcgacgg tgacgtggga attggcgctg
     2341 acgagataag gcgtctgcga gctcgatgga ccggtgatct ggctggaaat gatgaactgc
     2401 tcggcgtgca ggtcggagag cttcgcgtct tcgatcacca gttgatcgcc gccgccgagg
     2461 gcgatgacga cgtcgtcgtg ggattggtgc gcagcgttga gaacggcctg aaacgaggcg
     2521 aaatggaagt cacgaatgtc gacgcggtcg ccgggctgca ggtcggcgat ggtatcggtg
     2581 ccggtgccct ggcgaatgag gaaggtatca ttgtcggcgc cgccgaccag cacgtccttg
     2641 ccggggccgc cgaccaataa atctctgccg cggccgccga cgaggtgatc gttgcccgcc
     2701 tctccgacca acgcatcgtt gccgcgcccg ccgaagagat ggtcgttgcc gttgccgcca
     2761 aaaatcagat cgttgccgcg gccgccgaat gcatggtcat ttccgccgcc ggcgaaaatg
     2821 gtgtcgttgc cgccaaaccc gaagaagatt tcgccctggt cggttccatt gagcgtgtcg
     2881 tcgccgctcg ttccgagctt gagtctagat ttgatcgcca tctgacggct cccgcattag
     2941 tgatggaagg gtcgcgtcag tagcggggtt ggttgacaag cgggtaacgc cgccgtgaca
     3001 atcccgatgc tttccacagc cagggaattt tcttacgtct tgcagtatga tgaaaccacc
     3061 gcgaaaactt tggcctgagg ggttgtgcct tccgaaggct cggttccatt ttgattcgta
     3121 gcgtgcagcg aatcggaaga tgccaatgag tggcggaggg acgctgggat ccatggtttc
     3181 tccccttgtg gatggagccg aggcaagagg agaaatgcta aaatcgcgat atgaataatg
     3241 tgaccagagg cgcactgcga tcatccctct tcggcattct ggcgctagcg gcgctcctct
     3301 tcattcctgc cggcactctc gattattggc aggcatggct gtttatggcc gtgttcgtat
     3361 gcgcatcggc agccattggc gtctatctcg ccatccgcga tccgaaactt ctcgaacgcc
     3421 gcatgcgtgt gggccctcgg gcggagaaag aaccggcgca gaagatcatc atggtcttcg
     3481 cgacgctcgg attcattgta atgctcgtct ttcccgcgct cgatcgccgg ttcgggtggt
     3541 cagcggtgcc gggttccgtg tcggtactcg gggatgccct gatcgcgctg gggttcctgt
     3601 tcacattctt tgtattgagg gaaaacacct acagcgcgtc cacgattcag atcgccgaag
     3661 gccaaacggt catctcgacg ggtccgtatg cgcttgtgcg gcatccgatg tatgcgggcg
     3721 cgctcgtcat gctcatcggc ataccgctcg cccttggctc ctggtggggc ctcttcgcac
     3781 ttctcttcat cttgcctgtc ttgatctgga gactgctcga tgaggaacgg ttcctgcgac
     3841 agaaccttgc gggctacgcg gactatcagg cgaaggtgaa gtaccgcctc ctgccattca
     3901 tttggtaagg gcttagcagc ccctagttta ggtttgcgga caatcaatga agtgaatgcg
     3961 cagacccatg actgtccggt cgaactgcca tacgccgcca ttacgccacg tcgaagggaa
     4021 aagcgttgca ggatgcgggc cgatccgcca cctttgtagc ccatgccaga tttgtcattg
     4081 aaacccaacc tcgtccccct gcatgcttgg ctcatcgagg ctgggaccag cgacgtagcg
     4141 gcggaagcgc tgttcgacgg gttctgcggg cggttggccg cggccggcgt gccgatcgcg
     4201 cggggttttc tgtccattgc ggggctgcat ccgttgcggc gggcgtattc gctcacgtgg
     4261 catgacgggc gaatcgccga agtcaccgat ttcggccacg cgaccatggc tacggcggta
     4321 tggcagacaa gtccgttccg ccacatgctt gaaactaaga cgcgacgatt gcaccgacgg
     4381 ctgttaggcg acgacgctgt gctcgatttc gaggtcctgc gcgagttccg tgatgcaggc
     4441 atgagtgaat ggctagctct catgtacggg ttcggctgga gtgttgcgct gccagcgaca
     4501 aatcagttcg gcgtcgtttt atcgtggacc acttccgcgg ccgtcggacg cggtaacggc
     4561 tggagtgcta tggaactggc ggcgatcgag gagctgtctg ggacgctggc gctggcgatc
     4621 aagggcaata gcgcacctgt cgcagcgcac gacttgctcg tggcctatct cggtggcgat
     4681 gcggccgacc gcgtcagcgc cgggcaagtg cagcgcggct cagtcagccg gatcgccgct
     4741 gtcattctct gtgcggatgt gcgcggcttt accaacttcg ctgaggagac agcgcctgag
     4801 gaggtaaccc ggcgtcttaa cggcatcttc gactgtctcg gtgatccgat gcgcgcggcg
     4861 ggtggcgaaa tcctcaaatt tctaggcgac ggcgtcctgg ccgtgttcct gccaccagac
     4921 ggcgcaaacg tggcaatcgt gacgcccgcg gcgctggcgg cggcgcgcga gatcctggcg
     4981 cgagtcgagg cgctcaacgc cacagagacg gcggccggca atccggcgct cagcctggac
     5041 attgcgctgc acgctggcga agtcacgtac ggcaacatag gcaccgccga ccgcctcgac
     5101 ttcacagtaa tcggccctgc cgtgaatgag gcggcgcgcc tcgagggatt gtgcaaagag
     5161 ttggggatgc caatcctgat ttccaagtcg ttcgcgcagg ccgcggaggg agtgcgagat
     5221 caattgttgt cggtgggtcg gcacccgctg aggggcgtgc gcgagccaca ggaggtgttc
     5281 accatttgca cttgacggtg cagataggag cccgcggtat tatcagattt cgatctcttc
     5341 gggtccgcgc tggagtcgag ggccacattg cgaaggtcga agccgaagcg cgcaccgccc
     5401 atgcacacta agcatctcat cctaacattt tgcacctgcg cagccgcgac gatccatgaa
     5461 cgaaacgccc gcatggcgtc cgtctccgcc tccccgttga actttcgcac ggtgaagccg
     5521 tcgaagtgct cgttgtaagt gcggctatgg tcgcgcaaga gcccgagcgc ctgctgttcg
     5581 ccgacgaaga ggccgcggac gctgtcggag cggtagtgga cgccggcggc gtcgcggccc
     5641 atacggtcgt ggcatcgacg aacaaatctt tggcgcagat gaacaagtct ccggacgtgg
     5701 gcaaagcgac taagctatat cggcctgaag gtgatgtggg agaagcaaag aaccagcaca
     5761 cttctcccac cgccgcagag tgaagaggcc gagttcgatc cgctctgctg actgaggccc
     5821 cggcctggca tgcccccatc ccctccggct ggggcctctt actccgatgc tgtgaagtca
     5881 tgacctccga cgacagtctc tccctgatcc tgcgccgcct ggacgagatc gagcgcaagc
     5941 tcgaccacat gctggaagaa ctgcggaccc tcaacacgcc caccgccatc ggctattcgg
     6001 tgcgccgggg caatcttcca aagaaggact gaagaaaaga attagcccca gggtacgccg
     6061 ctgcgctggt tgagcccgcc cctcgtggcc ggcgcggcga gatgccggta taaagaattt
     6121 gaagcccgag acatgggccg cgttcatgaa cgatggccgc gcgttcgagg gctcgccgct
     6181 tcggccgagg gaggaacgct gatgctcacg cgacgcagcc tgctcgccgt ttccgccgca
     6241 tggctcttcg gcgcggccga cgccgcggcg caagactggc cgcagcggcc cgtgcgcgtg
     6301 atcgttccgt tcggacccgg cggcggcgcc gacatcatcg gtcgcatcgt cgcgcagtcg
     6361 ctgcaggaga agctcggtca gccggtggtg atcgaaaacc ggccgggcgc cggcggcacg
     6421 ctcggcaacg aggccgtcgc ccgcgccgac aaggacggct acacgctcgg gatcatgacc
     6481 gcgggccaga tcatcgcggc ggtgatgagc aagtcgctgc gctacgacac gctcaccgcg
     6541 ttcgatccgg tgtcgcaggt ggcgaccgcg gggctgatca tcgtcacgcg gcccgacttt
     6601 cccgccgacg acgtcaagca attgatcgcc gccgccaagg ccaatcccgg caagatctcg
     6661 ttcgccagcc cgggcttcgg cgccacgcag cacttcaccg gcgaattgtt ccggcagacc
     6721 gccggcgtca acatcttgca cgtgccgttc cgcacctcgc ccgaggcgat cgccgcagtt
     6781 ctcggcaagc aggtcgacgt gctgttcgat acggtttctg cggtgctggg gcaggtgcag
     6841 tcgggccagc tcaaggcgct cgcggtcacc ggcagggacc ggtttccggc ggtgccggac
     6901 gtaccggccg cgatcgaatc gggcgtgctt cccggctacg acgttaccac gtggtacggc
     6961 gtgttcgcgc cgcgcgacac gccgcctgcg atcatcgcca agctcaacac gatcatcaac
     7021 gaaagcctca aggaggacgc cgtgcaaaag cgcttgacca cggccggcgt ggtcgtgcgc
     7081 ggctcgaccg ccgaggcttt cggcgagcac atggcgcgcg agtttaagcg ctggaacacg
     7141 gtgcgcgagg cggcgggcat tccgcagcag tagc
//
LOCUS       DATLJT010000782         2957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_508970,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000782 DATLJT010000000
VERSION     DATLJT010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508970"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VM910_17765"
     CDS             complement(<1..423)
                     /locus_tag="VM910_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI64409.1"
                     /translation="MSRKMLTGSFVALITPFNKDGSVDFGAFRTLLKFQEDRGTSAVL
                     IMGSTGETSMLAPEEKKQIIVETAKMKTPRMPIFFGCTGNNTEATIANVRFAEANGAD
                     GAILAAPAYICAPESDIERFFLDVADASDLPLGIYNNPP"
     gene            1159..2148
                     /locus_tag="VM910_17770"
     CDS             1159..2148
                     /locus_tag="VM910_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVI64410.1"
                     /translation="MGFPPEKELSDKCRGAWFAAVLAVANVTALLPAAAETIATFTRN
                     PSNPILRGVRIGGEIAAKSLDAQIVHFIPRSEAPSEQLGLVEEVVRVKPDAIVFAPFD
                     PKAMVAAIDRLNAAGIPVTNVNERLAGGNVVAYVGTDDYQLALTTARYLINAMGKKGS
                     VVILEGPDNLPTSIARVKAYKDALKEFPDVKLLASKSANYARAPAIEVMKSFLRLYPQ
                     IDGVLAANDPMAIGAVEALRAANRKAPVVGINASKEVIDFVKSGDILGSGDYNGFVQG
                     CLGTQIAIRNLRKQPTPKEVNLKAVVMDKTNYQDYETPIERRTCPTLESVSAN"
     gene            complement(2220..2759)
                     /locus_tag="VM910_17775"
     CDS             complement(2220..2759)
                     /locus_tag="VM910_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64411.1"
                     /translation="MSFYRVFPVFSTAFAVLYLLAATFNYALFTYHPLLHELDLLARP
                     AKAGPAMYWYGWITTAAIGALVVAAVATFVTDQWERWVQRAIAFACAYAVFYVIANGV
                     AVFIYDRSSHELEFLKHWATPATGALAATVIASFFFPIRWTQRVWSGWTGVIPLSVML
                     VFVYLLRGWFIPSVGLILK"
     gene            complement(2756..>2957)
                     /locus_tag="VM910_17780"
     CDS             complement(2756..>2957)
                     /locus_tag="VM910_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64412.1"
                     /translation="VGIFDLKEHFSTVALGLLPAYWYYWRQPLSAERASIRKFLTTII
                     AFTIWWNFLVGHFTNNIRGLWS"
BASE COUNT          616 a          913 c          888 g          540 t
ORIGIN      
        1 cggcggattg ttgtagatgc cgagcggcag atcgctcgcg tccgccacgt cgaggaagaa
       61 gcgctcgatg tcgctttccg gcgcgcagat ataggcaggc gccgccagga tggcgccgtc
      121 ggcgccgttc gcctcggcga agcggacgtt ggcgatggtc gcctcggtgt tgttgccggt
      181 gcagccgaag aagattggca tgcgcggcgt cttcatcttc gcggtctcga cgatgatctg
      241 ctttttttcc tccggcgcga gcatcgacgt ctccccggtc gagcccataa tcagcaccgc
      301 ggaggtgcct cggtcttcct ggaacttcag caaggtccga aacgcgccga agtccaccga
      361 gccgtctttg ttgaacggcg tgatcagcgc gacgaacgag cccgtcagca tcttcctcga
      421 cattacggtc tccattcccg acttcgcgac cctgaatccc attgatcggt tcatcgctgc
      481 tttggcggcg gcgacagcga agcgatgcca agccggtcag tccgtattta tacgataccg
      541 cgccgcgctc gcctacggcg ttttgccacg aagcgcgcgc agttggcctt ttgcccgagc
      601 gcgaaattgg acatcctgtg gctccttctt cggagggaag gccatgcatg tgttgagagt
      661 gctcgttgcg atggctttgg ccggcgcaat gatgagttgc ggtcaaggtc aggggcccaa
      721 gggcgatccg gggccgcccg gccctcctgg cccgaaaggc gatcctggtc cacccggccc
      781 ccctttcggc attcgcatcg ttcgatcgaa ttgcgatact acgaactgca gcgtgcaatg
      841 cggtgacgac gagttgctct tgaccgccta ttgcggagtc agacgcaacg ccgccgtcat
      901 tcccaccgag cggtccgcaa cctgccgcag cccggtgccg gccaatagtc cgcttgtcgc
      961 cgcatgcgtg aaagtaccgc cgcaatagtc agccgggggt gacagcggtg cagcagcgcg
     1021 agacgcgaga accgcagcac agaccgttcg ggtcactccc agggctggag accatcgccg
     1081 aacgtttgct agagtcgcaa cagcgctcgg tctcggcgcg ggatctaatt ccacacgacg
     1141 cgtgcatgga gaaacatcat gggctttccg cccgagaagg aactctcgga taaatgccgt
     1201 ggtgcctggt ttgcagccgt gctggcggtc gcgaacgtga ctgcgctatt gccggccgcc
     1261 gccgagacca tcgccacctt cacgcgcaat ccgtccaatc ccattctgcg cggggtgcgc
     1321 atcggcggcg agatcgcggc gaagtcgctc gatgctcaga tcgttcattt catcccgaga
     1381 agcgaggccc cttccgagca gctcggcctc gtcgaggagg tcgtgcgcgt taagcccgat
     1441 gcgatcgtgt tcgcgccgtt cgatcccaag gcgatggtgg cagcgatcga caggctcaac
     1501 gcggccggca tcccggtgac caacgtcaac gagcggctcg ccggcggcaa cgttgttgct
     1561 tacgtcggaa ccgacgacta tcagctcgcg ctcacgactg cgcgctacct catcaacgcg
     1621 atgggcaaga agggaagtgt cgtgatcctc gaaggccccg acaatctgcc gaccagcatc
     1681 gcccgcgtga aggcgtacaa ggacgcgctc aaggagtttc ccgacgtcaa gctcctcgcc
     1741 tcgaagtcgg ccaactatgc ccgtgcgccg gcgatcgaag tgatgaagag ctttctgcgg
     1801 ctctatccgc agatcgacgg tgtgctcgcc gccaacgatc cgatggcgat cggcgccgtc
     1861 gaggcgctta gagcggccaa ccgcaaggcg ccggtcgtcg gcatcaatgc cagcaaagag
     1921 gtaatcgact tcgtcaagtc cggtgatatc ctcggtagcg gcgactacaa tggattcgtt
     1981 caaggctgtc tcggcaccca gatcgccatc cgcaatctgc gcaagcagcc gacgccgaag
     2041 gaagtcaacc tgaaggccgt cgtgatggat aagaccaact atcaggacta cgagacgccg
     2101 atcgaacgcc gtacttgccc gactttggaa agtgtctcgg ccaactaggc cgtgcactca
     2161 tgaatcgagg ctggcctgca accgtatttc cgagggcccg agcagtgaag catccatcgt
     2221 cacttcaata tcaagccgac cgatggaatg aaccagcccc tcaggagata gacgaaaacc
     2281 agcatgacac tcagcggaat gaccccggtc cagcccgacc acacccgttg ggtccatcgg
     2341 atggggaaaa agaagctggc aatgaccgtg gctgccaacg ctccggtcgc cggcgtcgcc
     2401 caatgcttca ggaattcaag ttcgtggctc gaccgatcgt agatgaagac cgcgacacca
     2461 tttgcgatta cgtagaatac ggcgtaggcg caagcgaacg cgatcgcccg ttggacccag
     2521 cgctcccact ggtctgtaac gaaggtagcg accgcggcca ccacgagcgc accaatggct
     2581 gccgtcgtga tccagccgta ccaatacatg gccggccctg ccttcgctgg tcgcgccagc
     2641 aggtcgagtt cgtggagcag cggatgatag gtgaaaagcg cgtagttgaa agttgccgcc
     2701 agcaaataca gcacggcaaa cgcggtcgag aacacgggaa aaacacgata gaaactcatg
     2761 accatagtcc ccgaatgtta ttggtgaaat gtcccaccaa aaaattccac cagatagtga
     2821 aggcaataat ggtggtcaga aattttcgga tgctcgcccg ttcggccgaa agaggctgcc
     2881 tccaatagta ccaataggcg ggaagcagtc ccaacgcgac cgttgaaaag tgctccttga
     2941 ggtcgaagat tccgacg
//
LOCUS       DATLJT010000783         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1427452,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000783 DATLJT010000000
VERSION     DATLJT010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427452"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..251
                     /locus_tag="VM910_17785"
                     /pseudo
     CDS             1..251
                     /locus_tag="VM910_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961761.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(248..1147)
                     /locus_tag="VM910_17790"
     CDS             complement(248..1147)
                     /locus_tag="VM910_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVI64413.1"
                     /translation="MFSTATLWIPFTLIAAAGQVARNAMQRSLTGQLGTWGATNIRFL
                     FGFPFSLMFFAMVVVTSGDRVPWPPASFWPWLLLGALSQIIATGLMLVAMNERSFVVT
                     TAYLKTEAIQTAVFGFVFLGDHLTVMKVAAILIATIGVVITALRSGGEKSFAEIRPTI
                     IGLVAAAAFALSAIGFRGAILTVEGVSFVTAASYTLVWGLFVQTVVLSIYLFWRAPDV
                     LKKILALWKPSMLAGFMGAFASQFWFLAFALTAAANVRTLALVEVLFAQAVAYYSFKQ
                     KIAARELLGTALIVIGVALLVAA"
     gene            complement(1289..2059)
                     /gene="ubiG"
                     /locus_tag="VM910_17795"
     CDS             complement(1289..2059)
                     /gene="ubiG"
                     /locus_tag="VM910_17795"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HVI64414.1"
                     /translation="MTTQDIRAGGTSTGGSTVDPAEIAKFSKLSEEWWNPSGKMAPLH
                     KINPMRLAYIRDAAARKFERNVKSLNCLSGLRVLDIGCGAGLLCEPLSRLGAQMIGVD
                     PSASNIAAARLHAEKGHLSIDYRCTTVEEMDLRERFDIVLAMEVVEHVSDVGLFLSRC
                     AAMLKPGGMMVVSTINRNWKSFALAIVGAEYVLRWLPRGTHQWDKFVTPDELAKYLLD
                     NRLVVTEQTGVVYSPFFDRWSLSSDADVNYMVIAEEMV"
     gene            complement(2074..2541)
                     /locus_tag="VM910_17800"
     CDS             complement(2074..2541)
                     /locus_tag="VM910_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HVI64415.1"
                     /translation="MGRYIDEILQPGEKVLYSTNAHWMFYLPGLAAWIVAVILFALSR
                     TTTVEGVILLCLASSAVVALVALYWTARAWFHQWTTETDVTDRRVVHKTGFIKRRTFE
                     MALDKIESVDVNQSILGRILDYGDVTIMGVGEGRESIKTIASPLAFRNSITAR"
     gene            2749..>3147
                     /locus_tag="VM910_17805"
     CDS             2749..>3147
                     /locus_tag="VM910_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HVI64416.1"
                     /translation="MGRLVMKFGGTSVANIDRIRNVARHVKREVDAGHDVAVVVSAMA
                     GKTNELVEWCREASPMHDAREYDAVVASGEQVTSGLLAIVLQGMGIQARSWQGWQIPI
                     KTSDAHASARILEIDGRELIHRFGDRKEVAV"
BASE COUNT          629 a          982 c          995 g          541 t
ORIGIN      
        1 tgcgacaggc cagttctccc atcccggtcc ggtctcggcc aaaagctggc tttttctgtt
       61 gttgtcgggc ctgggcaccg gcgcctcctg gctctgctat tttcgcgcgc tgaagcttgg
      121 gccagcgacg ctggtggcgc cgatcgacaa gctgaggcgt cgccttcctc ggtgagcgcc
      181 cctcgctcaa tggctggatc gggatcgtcc tgatcgctgc cggcgcagtc ctgatcgcgg
      241 tcaagagcta agcggcaacc agcagcgcca cgccgatcac gatcagggcg gtgccgagaa
      301 gttcgcgcgc cgcgattttc tgcttgaacg agtagtacgc gaccgcctgc gcaaagagga
      361 cctcgaccag ggccagcgtc cgcacattgg cggccgccgt cagcgcaaac gccagaaacc
      421 agaattggga cgcgaacgcg cccatgaaac ccgcgagcat cgagggtttc catagcgcaa
      481 ggattttttt cagcacgtcc ggcgcacgcc agaacagata gatcgagagc accaccgtct
      541 gcacgaacag tccccacacc agcgtgtagg aggcggcggt gacgaaggaa acgccctcga
      601 ccgtgaggat ggcgccgcgg aaacctatcg ccgacagcgc aaaggcggcg gctgcgacga
      661 ggccgatgat ggtcggcctg atctcggcaa agctcttttc gccgccagat ctgagcgccg
      721 tgatgacaac gccgatggtc gcgatcagga tcgccgcgac cttcatgacg gtgagatgat
      781 cgccgagaaa cacgaagccg aaaaccgcag tctgaatcgc ctcggtcttg aggtaggccg
      841 tggtcaccac aaaggagcgc tcgttcatcg caaccagcat caacccggtg gcgatgatct
      901 ggctgagggc gccgagcagg agccacggcc agaatgaggc cggcggccat ggcacgcggt
      961 cgccagacgt gaccaccacc atggcgaaaa acatcagcga aaacgggaag ccgaacagga
     1021 agcggatatt ggtcgcgccc caggtgccga gctgcccggt cagcgagcgc tgcatcgcgt
     1081 tgcgcgcgac ctggccggcg gccgcaatca acgtaaaggg gatccagagc gtggcggtgg
     1141 agaacatgga gggggaggac cggctgaaaa tggccagtga ccgtgccggg tgcggcgcaa
     1201 ggggtcaacg gtaagcgtgc ataactggga tgcaagcagc cttagacgcg ctgtcgtccc
     1261 tgctttcgca aggacgacgg cgatctcgtc acaccatctc ctcggcgatc accatgtaat
     1321 ttacgtcagc gtcggaggag aggctccagc gatcgaagaa ggggctgtag accacaccgg
     1381 tctgctcggt gacgacgagg cggttgtcca ggagatattt cgcaagctcg tcgggggtga
     1441 caaatttgtc ccattgatgg gtgccgcgcg gcagccagcg caggacatat tcggcgccga
     1501 cgatggcgag cgcaaagctc ttccagttgc ggttgatggt cgagaccacc atcatcccgc
     1561 cgggcttcag catcgccgcg cagcgtgaca gaaacaggcc gacatcgctg acatgctcga
     1621 ccacttccat cgccagcacg atgtcgaaac gctcccgcag gtccatctct tcgaccgtgg
     1681 tgcagcgata atcgatcgag aggtgaccct tttccgcgtg cagcctggcc gcagctatgt
     1741 tggaagcgga cgggtcaacg ccgatcatct gtgcgccgag ccgcgacagc ggctcgcaca
     1801 acagccccgc gccgcagccg atatcgagga cgcgcaggcc ggaaaggcaa ttcaggctct
     1861 tgacattgcg ctcgaacttg cgcgccgcgg cgtcgcggat ataggcgagc cgcatcggat
     1921 tgatcttgtg cagcggcgcc atcttgccgg acgggttcca ccactcctcc gacagcttgg
     1981 aaaattttgc gatctccgcc ggatcgaccg tcgaaccccc ggtggaagtg ccgcccgcgc
     2041 ggatgtcttg cgtggtcatg atgagctttg ctcctatcgc gcggtgatgg agtttcggaa
     2101 cgccagtgga gacgcgatgg tcttgatgga ttcccggccc tcgccgacgc ccatgatcgt
     2161 cacgtcgcca tagtcgagga tgcgccccaa aatgctttga ttgacgtcga cgctctcgat
     2221 cttgtcgagc gccatttcga aggtgcgccg cttgatgaag ccggtcttgt gcacgacgcg
     2281 ccgatcggtg acgtcggttt ccgtcgtcca ttgatggaac caggctcttg cagtccagta
     2341 gagcgcgacc aacgccacca cggccgacga cgcaaggcac aacaggatga cgccctcgac
     2401 cgtcgtggtc cgcgacaggg cgaacaggat cacggcaacg atccaggcgg caagtccggg
     2461 caaatagaac atccagtgcg cgttggtcga atacagcacc ttctcgccgg gctgcaggat
     2521 ctcgtcgata taacgcccca tctcactaaa accttgattc gtcccgcaaa ccacaacctc
     2581 tgcagaccgg gttctggcgt ggaacccgct tgcccccggc cgcaccgcta tgtatacgcg
     2641 cgtttcggcc ccaccgccta gcttcgccgg ggccttgaga acttaatagc gctggagctg
     2701 attcgctccc cgacaaacgc gaacctgaag ggaattacgc gcgtcgtcat gggccgcctc
     2761 gtgatgaaat tcggcggcac atccgtcgcc aacatcgatc gcatccgcaa cgtcgcgcgt
     2821 catgtgaagc gcgaggtcga cgccggccat gacgtcgccg tggtggtgtc ggcgatggcc
     2881 ggcaagacca acgagttggt ggagtggtgc cgcgaagcct cgcccatgca tgatgcgcgt
     2941 gaatacgatg ccgtggtggc ctccggcgaa caggtcactt ccggcctgct tgcgatcgtg
     3001 ctgcaaggca tgggcattca ggcgcgctcc tggcagggct ggcagatccc gatcaagacc
     3061 agcgacgcgc atgcctcggc gcgcatcctg gagatcgacg gccgcgagtt gattcaccgc
     3121 ttcggggacc gcaaggaagt cgccgtg
//
LOCUS       DATLJT010000784         3260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_508991,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000784 DATLJT010000000
VERSION     DATLJT010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508991"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..461
                     /locus_tag="VM910_17810"
                     /pseudo
     CDS             276..461
                     /locus_tag="VM910_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(770..>3260)
                     /locus_tag="VM910_17815"
     CDS             complement(770..>3260)
                     /locus_tag="VM910_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HVI64417.1"
                     /translation="IGSALINKLAGHFTLVGLDKIPARQPPPAAECVCIDLTSEEGMR
                     GAFARINTAYGKSIASVVHLAAYFDLTGEPSELYEQITVRGTERLLKHLRDFAVEQFI
                     FSSSMLVHAPTEPGTPIDEDSPLAPRWPYPQSKVDTEALIRAEHGNIPVVLLRLAGVY
                     DDRCHSAFLAQQISRVYEKQLISHVYPGDISHGQSALHLDDLMDGFQRLIEKRHELPP
                     ELILLLGEPQTFGYDTLQRAIGLELHGQEWRTLPIPKAIAKTGAWIENDVLQEEPFIK
                     PFMIDLADDHYQLDISRARELIDWTPTQTLIDVVPKMTKAVRADPPGWYRDNKLNAAQ
                     VAAARADVQGEKEGMNPDEHHKMMRHHVEAMRQMHLEMIWVHFLNILLGAWLVTSPFV
                     FGTFDQTSFPEAILRVTHERGLWDPAWRSTALAWSDIASGLLVMFFGALSLSQRFSWA
                     QWITTLVGIWLLFAPLVFWSPSAAVYANDTLVGALVIAFAVLVPMMPGMSHEGMMDHS
                     DVPIGWTYCPSTYLQRLPIIALGAVGFFIARTLAAYQMGHIGHVIDPFFGGTGGLNGT
                     ETIITSNVSKAWPIPDGGLGAVTYMFEILMGAMGDRRRWRTMPWMVTMFIFIVAPLGI
                     VSIYFIIIQPIVIGTYCALCLLAALANVIMIPYSLDELVATGQYLVQSHGRSASLLRT
                     FFMGGASPGSQQDKKPDFGAAWPAMAASAIRGVTMPWTLVASAILGVWLMFSRLVFGS
                     VPPLADSDHLVGALVVTVAVIAMAEVARALRFINVLFGAWLIVAPWLLSGGSTVAASV
                     EVVTGIALIALSLSRGRRSAEHYGSWDYYIA"
BASE COUNT          734 a          975 c          975 g          576 t
ORIGIN      
        1 ggtggctagc cggaacttgt tagaggcagg taccgtccgt cgagacggca tcgcccggat
       61 cgcttttcaa gcttcgtgcc aatttgcgcg gcgatccaaa aacccgtacg atttaaatgg
      121 tatcgcactg tgaaccggtg gcgcaagagc gaacgcgtga cagcgcatac cgcatgcccg
      181 aggcagcact gcaaaaaaat tgtgcaaaaa tcggcagaca agcaatcgca gggggttcgc
      241 tgtacgcgat cgtcgtcaca tcgccaccat tgccgcaggg caagcacatt accgtgctcg
      301 ccgccgcgtc aatgcaaaac gcgctcgatg acataaacac cgcattcact aagaacaccg
      361 atgtcaaggt gattgcgggc tacgcggcga gttcggcact tataaagcag attgcacagg
      421 gagcggcggc cgacgtattc gcctccgcca acctcgaatg gtggttccaa ggaagacgac
      481 gaaacaccca gataaccgat ccgggatatt tttccagcct gctgcgctcg cgccgcgacc
      541 ggccacgccg ccgccgcgcc accgaggagc gtgatgaact cgcggcgctt ataaagaaaa
      601 tgcatccaaa actcctttag aatcggattc gccgaaggaa ggtcccagga ccgactgccc
      661 tcggagaggg gtgaaaacgc gcgacagcca catcaccacc cgaatgaccg gagatgcaac
      721 ttagcgactc cggccgcgac cggccgtacg accagagcgc ccgcctacgt cacgcaatgt
      781 agtagtccca gcttccatag tgctcggcgc tgcgacgacc gcgcgacagg ctaagcgcga
      841 tcagcgcgat gccggtaacc acttcgaccg acgccgcgac ggtggagcca ccggaaagca
      901 gccagggcgc gacgattagc caagcgccga acaggacatt tatgaagcgc agcgcccgag
      961 cgacctcggc catggcgatc acggcgacag ttaccacgag cgctccaaca aggtgatcgc
     1021 tgtcggcgag cggcgggacg ctcccgaaca caagcctgct gaacatcagc caaacgccga
     1081 ggatcgcact tgcgacaagc gtccacggca tcgtcacccc gcgaatcgcg gacgccgcca
     1141 tggccggcca agctgctccg aagtctggtt tcttgtcctg ctggctgccg ggagaggcgc
     1201 ctcccatgaa gaaggtgcga agcaacgatg cgctgcggcc atggctctgc acgagatact
     1261 ggcccgtcgc caccagctca tcgagcgagt agggaatcat gatcacgttg gccagcgccg
     1321 cgagcaggca aagcgcgcaa tatgtaccga tcacgatcgg ctggatgatg atgaaataga
     1381 tactgacgat gccgagcggc gcgacgatga agatgaacat cgtgaccatc cacggcatcg
     1441 tccgccagcg ccgacggtcg cccatggcgc ccatcaaaat ctcgaacatg taggttacgg
     1501 cgccaaggcc gccgtctggg atcggccagg ccttggagac gttcgaggta ataatcgtct
     1561 ccgtgccgtt aagtccgccc gtgccgccaa agaacggatc gatgacgtgg ccgatatgac
     1621 ccatctgata agccgcgagc gtgcgcgcga tgaagaaacc cacggcaccc agcgcgatga
     1681 tcggcagccg ctgcaggtag gtggatgggc aataggtcca gccgatcggc acatcggaat
     1741 gatccatcat gccttcgtga ctcatccccg gcatcatggg gacgagaacc gcgaaggcga
     1801 tgaccagcgc gccgaccagc gtgtcgttgg cgtagacggc ggcgcttggc gaccaaaaca
     1861 cgagcggcgc gaacagcagc cagatcccca ctagtgtggt gatccattgc gcccaagaga
     1921 agcgctgcga caacgacaga gcgccaaaga acatgaccag cagaccgctc gcgatatcgc
     1981 tccaagcgag tgcggtgctg cgccaggccg ggtcccacaa tcctcgctcg tgcgtgacgc
     2041 ggaggatcgc ctctggaaag ctcgtctgat cgaaggtgcc aaagacaaac gggctggtca
     2101 caagccaggc ccccagcagg atgttgagaa agtggaccca gatcatctca agatgcattt
     2161 ggcgcatcgc ttcgacatga tggcgcatca ttttgtgatg ctcgtcgggg ttcatgcctt
     2221 ccttctctcc ctgcacgtcg gcccgcgccg cagccacctg cgcggcattg agtttgttgt
     2281 cgcgatacca gccgggagga tcggccctga cggctttggt catcttggga acgacgtcga
     2341 ttaacgtctg tgttggcgtc cagtcgatca gctcgcgcgc ccggctgata tcgagctgat
     2401 aatgatcgtc cgccaagtcg atcataaagg gtttgataaa tggctcctcc tgcaacacgt
     2461 cgttttcgat ccacgcgcca gtcttcgcga tagcctttgg gatgggcagc gtccgccact
     2521 cttggccatg gagttcaagg ccgatggcac gctgcagcgt gtcatagcca aaggtctgcg
     2581 gctcgcccaa aagcaagatc agttccggag gtagctcgtg ccgcttttcg attagccgct
     2641 gaaacccgtc catcaggtca tcgagatgca acgccgattg gccatggctg atgtcgccgg
     2701 gatagacgtg actgatgagt tgtttctcat agacgcgact gatctgttgc gcgagaaagg
     2761 cagaatggca acgatcgtca taaacgccgg ccagccggag caacacgacc gggatgttgc
     2821 cgtgttccgc acggatcagc gcttcggtgt ccaccttgga ttgcgggtaa ggccatcgtg
     2881 gcgctagagg tgagtcttcg tcgatcggcg ttcccggctc tgtcggcgca tgcacgagca
     2941 tgctcgaaga aaagatgaat tgttcgaccg caaagtcccg caagtgtttc aacaaacgtt
     3001 cggtgccgcg gacagtaatt tgctcataaa gctcgctggg ctcgccggtc agatcgaaat
     3061 aagccgcgag atgaacgacg ctggcaatgc tcttgccgta agcggtgttt atccgcgcga
     3121 acgcgccccg catgccttct tctgaggtga ggtcaataca aacgcattcg gctgcgggag
     3181 gcggttgacg cgcggggatt ttgtcgaggc cgaccaaggt aaaatggccg gcgagcttat
     3241 tgatcaacgc cgatccgatg
//
LOCUS       DATLJT010000785         4828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2577338,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000785 DATLJT010000000
VERSION     DATLJT010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577338"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VM910_17820"
     CDS             <1..665
                     /locus_tag="VM910_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64418.1"
                     /translation="PTLPSQAAEQAQPQLPTEIPPAQAQSHLPAVQSEAAADASSLAL
                     QLTPALTAADEEFCPRTISFTIPGGGPGNPGVQVTAVEHQENGVKTGTIDFTVDVLGD
                     ADLRGLFFQFNEAHLNGLTVTGGDGLITKTVIQANSVINLGQGAEMNGAANPFDVGIR
                     WGTPGPNPDNINFPVHFTLSNAANKLTLDDIAHQQFGVKIGAAGAKTQSLRPSPRPPR
                     MR"
     gene            662..1330
                     /locus_tag="VM910_17825"
     CDS             662..1330
                     /locus_tag="VM910_17825"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HVI64419.1"
                     /translation="MNDNAPPPSIFEDGASGLGDPSHTALGKTGLITVLSNDKDLDPG
                     DTLTITDTHGAQHGTVQIVDGPDADNLPGDAVFYTPFADYSGPDSFLYCVSDGHGGQD
                     NATVNLNVEAVADIPDLQVSVALGATINEYIVNVTATQTDLDGSEFIDRISWTGVPDG
                     VTITPVGDPNPSDQPDHVRGDGSSQAGHGFQSQHHGRLGREVQRRYRADDRRPGDQTR
                     LQPQ"
     gene            2463..4238
                     /locus_tag="VM910_17830"
     CDS             2463..4238
                     /locus_tag="VM910_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HVI64420.1"
                     /translation="MSTKTDISGGGNKSSKSELAAALKSCRGSFIGVGLVSSVTNILM
                     LTGPFFMLELYDRVLPSRSVPTLIGLGALAAVLFAFQGLLEWIRGRILVRIGGALDET
                     LSLRVHDALVRAPLKGKVGGDGLQPLRDLDQVRSFLSSPGPMALFDFPWIPFYLGICF
                     LFHVLIGLAATVGGLILIFLTYLTEVRTRGPTKAAVTFAATRNALAEAGRRNAEVLQA
                     MGMAGRVGGVWARTNREYMASQRRATDVAGGLGALSKVLRLALQSAVLGLGGYLAIRQ
                     EATAGIIIASSILTARALAPVELAIANWKGFVAARQSWRRLTELLILLPAREQPMALV
                     PPSAELLVEAMSLVAPGSRTFVVRDVNFALKAGSGLGIIGPSAAGKSSLARGLVGVWV
                     PVQGTVRLDGAALDQWSCEDLGRHIGYLPQDVELFDGTVAENIARFHAQADPMAIIAA
                     AKQAGVNDLILRLPQGYETRIGEGGMALSAGQRQRVALARALYGDPFLVVLDEPNSNL
                     DHEGEEALTKAILGVRTRGGIVIVVSHRATALASVDLILVMKEGKSQAFGPRDEVMAK
                     IVRRFPAPVAPALPLRVVAESGQAS"
     gene            4235..>4828
                     /locus_tag="VM910_17835"
     CDS             4235..>4828
                     /locus_tag="VM910_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVI64421.1"
                     /translation="MTEQKIQTKSHESLRRHLLSAAALIVILVFGMGGWAATVELSGA
                     VITSGQVVVDSNVKKVQHPTGGIVGEVRVRDGYRVKEGDIVVRLDETQTKANLLIVTK
                     SLDELAARQAREEAEREGAGQVLFPRELLARFDDPDVAHVVSEERKLFAIRREAREGQ
                     KAQLRERIAQLKEEIRGYEAQQVSKDEQIDWIKKELAG"
BASE COUNT          965 a         1493 c         1482 g          888 t
ORIGIN      
        1 caccgacact gccgtcgcag gccgcagaac aggcgcaacc tcagctgccg acagagatac
       61 cgccagctca agcgcagtcg caccttccag ccgtccaaag cgaggctgcg gctgatgcgt
      121 cgtcactcgc actgcaattg acgccagcgt tgacggcggc cgacgaggag ttttgtcctc
      181 gcaccataag tttcacgatc ccgggcgggg gcccgggcaa tccgggcgtg caggtcaccg
      241 cggtcgagca ccaagaaaac ggcgtaaaaa ctggcacgat cgacttcacc gtggatgtcc
      301 tcggcgacgc cgatctgcgt ggcctgttct tccagttcaa cgaggctcac ctcaacggcc
      361 ttacggtgac cggtggggat ggactgatca ccaagaccgt gatccaagcc aacagtgtga
      421 tcaacctcgg acagggcgcc gagatgaacg gcgcggctaa tccgttcgac gtcggcatca
      481 ggtggggaac accgggaccc aacccggaca acatcaattt tccggtgcac ttcaccctct
      541 caaatgccgc gaacaagctc accttagacg atatcgccca tcagcaattc ggggtgaaaa
      601 tcggcgctgc gggggcaaag acgcaaagct tacgaccatc gccccggccg ccccggatgc
      661 ggtgaacgac aacgcgcccc cgcccagcat ttttgaggac ggtgccagcg ggctcggcga
      721 tccctcccac accgcgttgg gcaagaccgg gctgatcacg gttctctcaa acgacaagga
      781 ccttgatcct ggcgacacac tgacaatcac cgacactcat ggcgcccagc acggcacagt
      841 acagattgtc gacgggcccg atgccgacaa tctgcccggg gatgcagtct tctacacgcc
      901 attcgcagat tacagcgggc cggacagctt cctctattgc gtctccgacg gacatggcgg
      961 ccaggacaac gccaccgtga acctgaacgt cgaagctgtc gctgacatcc ctgatctgca
     1021 ggttagcgtc gctctaggcg ccacgataaa cgagtatatt gtcaacgtca ccgcaaccca
     1081 gaccgatctg gacggctccg agttcatcga ccgcatcagc tggaccgggg tccccgacgg
     1141 cgtcaccatt acacccgtcg gcgatcctaa tccctcggac caaccggatc acgttcgtgg
     1201 tgacggttcc tcccaagcag gacacggatt tcaatctcaa catcacggcc gtctcggaag
     1261 agaagtccaa cggcgatacc gagcagacga ccgtcgccca ggagatcaaa ctcgacttca
     1321 accacaatga gacccagcag accttctcga cgacgaacca gagcatctgg gatccgagcc
     1381 ttccggcggc gtttaacgac gatcggtttc tgggcttcca cgacgccgat agtgctaaag
     1441 tcgacatcga actggcctcg gcgggcggca gctacgacat cacgatcggg ttccagtcca
     1501 ccctgcacgc gacgctgggc gacatcaagg cgaccctacc gtacaacatc accatcgaca
     1561 cctcgtacaa cgtaacgacc gactcgctgc tgatcgatcc gacggccgtg ctggatccca
     1621 gcgccttctt cgagacccac gggccgggcg gatcctacaa actcgatttc gtgtgtcttg
     1681 ccgagctgtc ggcgtttctc cacaacccta tccatgactt cgataaaagc gtctcggtcg
     1741 acctcggttt caacctgctg gacatcaaca ccaacacgtt caacaaaacg atcgatatcc
     1801 cacccccacc gaacacagtt accaccgtca cgctcaactg gcccaaggtg gacacgaacg
     1861 gggcgccggt cgatgctgac acgctgcact cggacggcac cagccccgat atcgtccacc
     1921 tggacgtcga cgtgatcgcc ttggcttgcg ccgtcctcgg cattccaaat ccgcttgacc
     1981 tcggcttcgt cgacctactg gcgctccatg tcaacggcgg cgtagacgtg gcgcaacact
     2041 tcgacttgaa ctcggtcctg aaagcccaag acgcatcgat tgtcctcgag gatggatcgt
     2101 cactgccgtt cgccttcggc agcccgctgc ccatcatccc gaacgcctca agtcacgacg
     2161 ccaaccacga cggccagatc gccttcagtc tgcacctcac gccggatgcg acgctgcaaa
     2221 acgagaccga tctcggcttc cacgtcggcg ccgacctcaa agtgctcgac ttcggcgatc
     2281 cgttcggcgc gctgttttct ctgggcggcg atctgcccct cggagaaatc cccatctacc
     2341 atccagacgc ctttgcgctg ggcggcttca actcgcagga ctaccacttc caggtctagg
     2401 cgtgcagcgc cggccacgac gcattgtggt cgtggccggc ggccccggcc agaaggcgtt
     2461 acgtgagcac aaaaaccgat atctccggag gcggtaacaa atcctcgaaa tctgagcttg
     2521 cagcggcgct caagtcgtgt cgcggcagct ttatcggcgt tggcctcgtg agcagtgtga
     2581 ccaacatcct gatgctcacg ggcccattct tcatgctcga gctctacgac cgggtccttc
     2641 cgagccggag cgtacctacg ctcatcggct tgggggcgct tgcggcagta ctgttcgcgt
     2701 ttcaaggact gctcgagtgg atccgggggc gcatcctggt gcgcatcggc ggcgcgctag
     2761 acgagactct gagcctgaga gttcatgatg cattggtgcg ggcgccgttg aagggcaagg
     2821 taggcgggga tggtcttcag ccgctgcgtg atctcgatca agttcgttcg ttcttgtcga
     2881 gtccgggacc aatggcgctc ttcgattttc cctggatacc gttctacctc ggtatttgct
     2941 tcttgtttca tgtcttgatc ggcttggccg cgaccgtggg cggcctgatc ttgatctttc
     3001 tcacatatct gacggaggtc cgcacccgcg gcccaaccaa ggccgcggtg acctttgctg
     3061 caacgcgtaa cgcgctagcg gaggcgggcc ggcgcaatgc agaggtgttg caggccatgg
     3121 gtatggccgg tcgggtaggc ggcgtatggg ctcgtaccaa ccgcgaatac atggcgagtc
     3181 agcggcgggc gaccgatgtg gcgggcggcc tcggggctct gtcgaaggtg ctgcggctcg
     3241 ccctgcaatc ggcagttctg ggactcggcg gctatcttgc gatccgtcag gaggccactg
     3301 ccggaatcat catcgcgagt tcgatcctga cggcgcgggc gctggcgccg gtcgaactgg
     3361 cgatcgccaa ttggaaggga tttgtcgcgg cgcggcaaag ctggcggcgg ctgacggagc
     3421 tattgatttt gttgcccgcg cgagaacagc cgatggcgct ggtgccaccg agcgccgagc
     3481 tcttggtcga agccatgagc ctcgtggcgc cggggagccg caccttcgtg gtgcgggatg
     3541 tcaactttgc gctcaaagcg ggaagcgggc tgggaatcat cgggccgagc gccgcgggga
     3601 agtcgtcatt ggcgcgaggg ttggtgggag tatgggtgcc tgtacagggc acggtccggc
     3661 tggacggagc cgcgctcgat caatggagct gcgaagacct cggccgacac atcggctacc
     3721 tgccccagga cgtagaactc ttcgatggga ccgtggcgga gaacattgcg cgttttcatg
     3781 cacaggcgga tccaatggcc atcatcgcgg cggcgaagca ggccggcgtc aacgatctga
     3841 tcctgcggct accacaaggc tacgagacgc gcatcgggga agggggaatg gcgctctcgg
     3901 ccggacagcg gcagcgtgtt gcgcttgcgc gcgccctcta tggcgacccg ttcctggtcg
     3961 tcttggatga accgaactcg aatctggatc atgaaggtga agaggcgttg accaaggcga
     4021 ttttgggagt gcggacgagg ggcggcatcg tgattgtcgt gtcgcatcgg gcgaccgcgc
     4081 tggcgagcgt cgaccttatt ctggtgatga aagagggcaa gtctcaggcc ttcggtccgc
     4141 gagacgaagt gatggccaaa atcgtccgcc gttttcccgc cccggtggcg cctgcactgc
     4201 cgctcagggt ggttgccgag agtggacaag catcatgacg gagcagaaga tccaaacaaa
     4261 gagccacgag tctcttcgcc gccacctgct ttccgccgcg gcccttatcg tcattcttgt
     4321 ctttggaatg ggcggctggg ctgccacggt ggaactttcg ggcgcggtga tcacgtctgg
     4381 gcaggtagtc gtcgattcga atgtcaagaa ggtgcagcac ccgaccggag gcatcgtcgg
     4441 agaggttagg gtccgcgacg gctaccgcgt caaggaagga gatatcgtgg ttcgtctcga
     4501 cgaaacgcag acaaaagcga acctgctgat tgtgacaaaa agcctagacg agcttgctgc
     4561 gcggcaagca cgagaggaag ccgagcggga gggggccggc caagttctct ttccgcgcga
     4621 gctcctcgcg cgctttgacg atcccgatgt ggcgcatgtg gtgagtgaag agcgcaaatt
     4681 gttcgcaatc cgtcgcgagg cacgggaagg gcagaaggcg cagctcaggg agcgcattgc
     4741 ccagctgaaa gaagagatcc gtgggtatga agcgcagcag gtctccaaag acgagcagat
     4801 cgattggatc aagaaggagc tcgcgggg
//
LOCUS       DATLJT010000786         4451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4185127,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000786 DATLJT010000000
VERSION     DATLJT010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4185127"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..2129
                     /gene="nuoG"
                     /locus_tag="VM910_17840"
     CDS             54..2129
                     /gene="nuoG"
                     /locus_tag="VM910_17840"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HVI64422.1"
                     /translation="MTKLIIDGKEIDVPPEMTLLQACEAAGAEIPRFCYHERLSIAGN
                     CRMCLVEVKGGPKPVASCAWGVRDCRPGPKGEPPEISTRSPMVRKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQAMGYGVDTSRFAENKRAVEDKYLGALVKTSMNRCIQCTRC
                     VRFSAEVCGTPEMGATGRGEDMEITTYLEQALSSELQGNLVDICPVGALTSKPYAFAA
                     RPWELGKTQSIDVMDGVGSSIRVDTRGREVMRILPRINEAVNEEWISDKTRHVVDGLR
                     AQRLDRPYIRENGKLRPASWSEAFNAIAAKAARTDGKRIGAIAGDLSGVEEMFALKDL
                     LSRYGSVNLAVQGGDNFDLNVGRASYIFNPTIAGIDQADALLIVGSNPRREAAVLNAR
                     IRKRWRTGQLKIGLIGEKADLTYAYDYLGAGTDSLAELASGKGAFADVLKNAKHPIVL
                     IGAGATGRHDGRAVLAVAAKLAAGLGVVKDGWNGFAVLQDTASRAGALDIGFAPSAGG
                     LTTAQMTAFGTLDVLFLLGADEIKVPDGTFVVYIGTHGDNGAHRADVILPGAAYTEKS
                     AIYVNTEGRVQITGRAAFPPGEAREDWAIIRALSEVLGKKLPYDSLAALRQSIFKAVP
                     HLMRVDQIAAGNAADIEKLAARGGSLEKAPFKNVVEDFYLTNPIARASFVMAECSRLA
                     SERMLRAAE"
     gene            2141..3211
                     /gene="nuoH"
                     /locus_tag="VM910_17845"
     CDS             2141..3211
                     /gene="nuoH"
                     /locus_tag="VM910_17845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVI64423.1"
                     /translation="MADFFASSFWTGFLWPLIIMVAESVLLLVVLLVAIAYILLADRK
                     IWAAVQIRRGPNVVGPWGLFQSFADLLKFVVKEPIIPAGANKGVFLLAPLVSCVLALA
                     AWAVIPTNLGWAIADINVGILYIFAISSLSIYGIIMAGWSSNSKYPFLAALRSAAQMV
                     SYEVSIGFVFITVLLCAGSLNLSAVVEAQNVRGLASLIGLPKLTILNWYVWPLLPMFV
                     VFYVSALAETNRPPFDLVEAESELVAGFMVEYGSTPYLLFMLGEYVAITTMCAMATIL
                     FLGGWLPPVALPPFTWIPGVIWFSLKLFLMFFLFAMAKAIVPRYRYDQLMRLGWKVFL
                     PLSLAAVVVVAGVLHFADIAPK"
     gene            3219..3713
                     /gene="nuoI"
                     /locus_tag="VM910_17850"
     CDS             3219..3713
                     /gene="nuoI"
                     /locus_tag="VM910_17850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HVI64424.1"
                     /translation="MSVNVKATARSLLLSEFVSAFVLAMRYFFQPKPTLNYPFEKGPI
                     SPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMV
                     KCIYCGLCQEACPVDAIVEGPNFEFAAETREELYYDKAKLLANGDRWESQIAKAIELD
                     APYR"
     gene            3723..4361
                     /locus_tag="VM910_17855"
     CDS             3723..4361
                     /locus_tag="VM910_17855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVI64425.1"
                     /translation="MILPALFFYLFAGVCVASAVMVIASRNPVHSVLFLILAFVNASG
                     LFVLMGAEFLAMILIVVYVGAVAVLFLFVIMMLDVDFTELREGFLEYLPIGLVIGGVF
                     LAELLLVGGGWVINPGTAKSITAAIPANVSNTEALGLVLYTKYIHYFQLAGLVLLVAM
                     IGAIVLTLRHKAFVKRQDINVQNARTPELAMSLRKVASGQGLLDADSAEWVK"
     gene            4358..>4451
                     /locus_tag="VM910_17860"
     CDS             4358..>4451
                     /locus_tag="VM910_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HVI64426.1"
                     /translation="MTIGLGHYLAVGAILFTLGILGIFLNRKNII"
BASE COUNT          741 a         1340 c         1438 g          932 t
ORIGIN      
        1 gacgacatcg gcattcgcga ccccgtcaac atggtcgcgg cggagtagat gcaatgacca
       61 agctgatcat cgatggcaaa gagatcgatg ttcccccgga aatgacgctg ctgcaggcgt
      121 gtgaggctgc gggcgccgag attccgcgct tctgctatca cgagcggttg tcgatcgccg
      181 gcaattgccg gatgtgcctg gtcgaggtga agggcggccc caagccggtc gcgagctgcg
      241 cctggggcgt gcgcgactgc cgtcccggtc ccaagggcga gccgccggaa atctccacgc
      301 gctcgccgat ggtgaggaaa gcgcgcgaag gcgtgatgga atttttgctc atcaaccacc
      361 cgctggactg cccgatctgc gaccagggcg gagagtgcga cctgcaggac caggcgatgg
      421 gctacggcgt cgacaccagc cgttttgccg agaacaagcg cgcggtcgag gacaaatatc
      481 tgggggcgct ggtcaagacc tcgatgaacc gctgcatcca atgcacgcgc tgcgtccgct
      541 tctcggccga agtatgcggc acgcccgaaa tgggtgcgac cggccgtggc gaggacatgg
      601 agatcaccac ctatctggag caggcgctga gctccgagct gcagggcaat ctcgtcgata
      661 tctgcccggt gggcgcgttg acctcgaagc cttatgcctt tgccgcgcgc ccctgggagc
      721 tcggcaagac gcaatcgatc gacgtcatgg acggcgttgg ttcttcgatc cgcgtcgata
      781 cgcgcgggcg cgaggtgatg cggatcttgc ctcgcatcaa cgaggccgtg aatgaggaat
      841 ggatctccga caagacgcgg catgtcgtcg acgggctgcg cgctcagcgt ctggatcggc
      901 cctatatccg cgagaacggc aagctccgcc cggcctcctg gtcggaggcg ttcaacgcga
      961 tcgcggcaaa agcggcgcgc acagacggca agcgcattgg tgcgatcgcc ggcgatcttt
     1021 ccggcgtcga ggaaatgttc gccctgaagg atctgttgtc gcgctatggt tcggtcaacc
     1081 tggcggtgca gggtggcgat aacttcgatc tcaatgtcgg ccgcgcctcc tatatcttca
     1141 atccgacgat tgccggcatc gatcaggcag acgcgctctt gatcgttggt tccaatccgc
     1201 gccgggaagc ggccgtcctc aacgcgcgca tccgcaagcg ctggcgtacg ggccagctca
     1261 aaattggttt gatcggcgaa aaggccgatc tgacctacgc ctatgactat ctcggtgcgg
     1321 gcacggattc gctcgctgag ctcgcgagcg gcaagggcgc gtttgcggac gtgttgaaaa
     1381 atgccaagca tccgatcgtg ctgatcggcg ccggtgcgac gggccgtcac gacggcaggg
     1441 cggttcttgc ggtcgctgca aagcttgcgg ccggtcttgg cgtcgtcaag gacggctgga
     1501 acggatttgc ggttctgcag gatacggcgt cgcgcgccgg cgcgctcgat atcggctttg
     1561 cgccgtcggc gggtggactg acgacggcgc agatgacggc gttcggcacg ctcgacgttc
     1621 tattcctgct cggcgccgac gagatcaagg tgcctgatgg cacttttgtg gtctatatcg
     1681 gtacccacgg cgacaacggc gcgcatcggg cagatgtgat cctgccgggc gcggcctaca
     1741 cggaaaaatc cgcgatctac gtgaataccg agggcagggt gcagatcacg ggtcgcgcgg
     1801 cctttccgcc cggggaagcg cgcgaagact gggccatcat tcgtgcgctg tcggaggttt
     1861 tgggcaagaa gctgccgtac gattcgctgg cggcgctgcg gcagtcgatc ttcaaagccg
     1921 tgccgcattt gatgcgcgtc gaccagatcg cggccggcaa tgcggccgat atcgaaaaac
     1981 tggcggccag gggcggcagc ctcgagaagg cgcccttcaa gaacgtggtt gaggatttct
     2041 atctcaccaa cccaatcgct cgagcgtcgt tcgtaatggc ggaatgttcg cgcctggcgt
     2101 ccgaacgaat gcttagagct gcggagtaag caggacgatg atggcagatt tcttcgcaag
     2161 ctcattctgg accggcttcc tgtggccgct gatcatcatg gtcgcggaga gcgtcctgct
     2221 gctcgtcgtt ctcttggtcg cgatcgccta catcctgctt gccgaccgca agatctgggc
     2281 cgcggtgcag atccggcgtg gcccgaacgt ggtcggccca tggggcctat tccagtcgtt
     2341 tgccgatctt ctgaagttcg tggtgaagga gccgatcatt ccggccggcg ccaacaaggg
     2401 cgtgttcctg ctggcgccgc tggtgtcctg cgtgctcgcg ctcgccgcct gggcggtgat
     2461 accgaccaat ctcggctggg cgatcgccga catcaatgtg ggcattctct acatcttcgc
     2521 gatttcctcg ctgtcgatct acggcatcat catggccggc tggtcgtcga actcgaaata
     2581 cccattcctc gccgcgctgc gctcggcggc ccagatggtg agttacgagg tctcgatcgg
     2641 cttcgtcttc atcacggtgc tgctgtgtgc aggctcgctc aatctctcgg cggtggtgga
     2701 ggctcagaac gtccgcgggt tggcaagcct gatcggcttg ccgaagctca cgatcctgaa
     2761 ctggtatgtc tggccgttgt tgccgatgtt cgtggtgttc tacgtctcgg cgctggcaga
     2821 gaccaaccgg ccgcccttcg atctcgtcga agccgaatcc gaattggtcg cgggcttcat
     2881 ggtcgaatac ggctcgacgc cgtatttgct cttcatgctc ggcgaatacg tcgccatcac
     2941 cacgatgtgt gccatggcga ccatcctgtt cctggggggc tggctgccgc cggtcgcact
     3001 gccgcccttt acctggattc cgggcgtcat ctggttctcg ctgaaactgt ttctcatgtt
     3061 cttcctgttc gcgatggcga aggcgatcgt gccgcgctac cgctacgatc aactgatgcg
     3121 gctcggctgg aaggtgtttt tgccgctgtc gctggcggcg gtcgtcgtcg tcgccggcgt
     3181 gctgcatttc gccgacatcg cgccgaagtg aggtcgccat gagtgtcaat gtcaaggcta
     3241 cagcccgctc gctcctgttg tcggagttcg tttcggcatt cgttctcgcc atgcgctact
     3301 tcttccagcc gaagccgacc ctgaactatc ccttcgagaa ggggccgatc tccccccgct
     3361 tccgcggcga gcacgcgttg cggcgctacc ccaatggcga agaacgctgc atcgcctgca
     3421 agctgtgcga ggcgatctgc cccgcgcagg cgatcaccat cgaggccggc ccgcgtcgca
     3481 acgacggcac ccgccgcacg gtgcgctacg acatcgacat ggtgaagtgc atctattgcg
     3541 ggctctgcca ggaggcctgt ccggtcgatg ccatcgtcga agggccgaac ttcgaatttg
     3601 cggccgagac gcgcgaggaa ctgtactacg acaaggccaa gctgctcgcc aatggcgacc
     3661 gctgggaaag ccagatcgcg aaggcgatcg aactcgatgc gccgtatcgg tgaggtgagc
     3721 gcatgatcct tcctgcactt ttcttttacc tgtttgccgg tgtgtgcgtc gcctccgccg
     3781 tgatggtgat cgcctcgcgc aatcccgtgc attccgtgct gttcctgatc ctcgccttcg
     3841 tcaacgcttc cggacttttc gtgctgatgg gtgccgagtt tttggcgatg atcctgatcg
     3901 tggtctatgt cggcgcagtc gcggtgctgt ttttgttcgt gatcatgatg ctcgacgtcg
     3961 attttaccga gctgcgcgag ggctttctgg aatacctgcc gatcgggctg gtgatcggcg
     4021 gggtgtttct cgcggaattg ctcctggtcg gcggcggctg ggtcatcaac cccggcacgg
     4081 cgaaatcgat cacggcggcg atcccggcca atgtcagcaa taccgaggcg ctcgggctcg
     4141 tgctctatac gaagtacatc cactactttc agcttgccgg cctggtgctg ctggtcgcga
     4201 tgatcggcgc catcgtgctg accctgcgcc acaaggcctt cgtcaagcga caggacatca
     4261 acgtgcagaa cgcgcggacg cccgagcttg ccatgagttt gcgtaaggtg gcttccggcc
     4321 agggactgtt ggacgcggat tcggcggagt gggtgaaatg accatcgggc tggggcacta
     4381 tcttgcggtc ggcgcgatcc tgttcacgct cgggatcctg ggcattttcc tcaatcgcaa
     4441 gaacatcatc g
//
LOCUS       DATLJT010000787         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3496287,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000787 DATLJT010000000
VERSION     DATLJT010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3496287"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VM910_17865"
     CDS             <1..400
                     /locus_tag="VM910_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI64427.1"
                     /translation="LIGHSNGGDISMYFAKLHPEMVKKVVTLDNLRVPFLTDGKIKIL
                     SFRSKDPVFKADPGVVPDDDVCAKAGITVVKTEFQHNELSDRGPDSVKASIEAKVHSF
                     LDEEEGPSSPLGLLTANAPNPPASATSSKN"
     gene            complement(599..>2459)
                     /locus_tag="VM910_17870"
     CDS             complement(599..>2459)
                     /locus_tag="VM910_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVI64428.1"
                     /translation="NPMLQAWFVRTGHPAGPDPYFLYASSNIGSFLALLSYPVLLEPM
                     FTLRTQNLMWTAGYGLLILLIAGCGVVLLRSPKAAADLQREDTQALPPGWVLRARWIF
                     LAAVPSGLLIAVTAHISTDVAAAPLLWVLPLSLYLLTWVLVFQSRPLLPHRWMLTLQP
                     IAIAGVAVLLAVGGEQNLLLTLGGHQLCFFVIAMACHGELARTRPPAKYLTGFYVALS
                     FGGMLGGLFAGLISPYAFSWVAEYPILLALAALCRPPSSERWPQWSRWYWLLLAALAI
                     ALIAPTVAGGGKVFAWLEGRRVWVAGSVGVLAGLIALALKASRWKVFATVVLALVLIR
                     FYPADDGRVETVRSFFGVHKIVMTSHGQYHVLMHGTTIHGAQKVLNNDGSPLEGRPEP
                     ITYYHTDGGIGQAITAIRERKGGPLKVAVIGVGAGTLACASEPGETWKFFEIDQTMVD
                     TARDPKYFTYMRSCEPDAKPVIGDARLTFAREPDGFYDLIIVDAYSSDAIPIHLATEE
                     AMKIYKSKLAPQGAVVMHVSNRHLELESVVVGIADANDLKSWVYDEDSRRDDEYIFAT
                     DVVVCAREEADVGNLASSDKWAETEPTEGQRVWTDDYSNILGAVFRRLKFGEE"
BASE COUNT          516 a          787 c          745 g          411 t
ORIGIN      
        1 cctgatcggg cactcgaatg gcggcgacat ctcgatgtat ttcgccaagc tgcatcccga
       61 aatggtcaag aaggtcgtca cgctcgacaa tctgcgcgtg ccgttcctga ccgacggcaa
      121 gatcaagatc ctgtcgttcc gttcgaaaga tcccgtgttc aaggccgatc ccggcgtcgt
      181 tcccgacgac gatgtctgcg ccaaggccgg catcaccgtg gtcaaaaccg aatttcagca
      241 caacgagttg agcgatcgcg gccccgacag cgtcaaggca tcgatcgagg caaaggtgca
      301 ctccttcctc gacgaagaag agggcccctc atcgcctttg ggactcttga ccgccaacgc
      361 tccgaatccg ccagcctcgg ccacaagcag caagaattga acgaagctaa agcgtgattt
      421 cctcatctcc cccttaaaaa ggggaggtcg ctttgctccg tcaggagcaa agcgggaggg
      481 gatcaattga ccctctcgct tctccaatcg accctctccc tgccctgaaa gggaggaggg
      541 atacactttc accctgagcg cagttcaacc tgtactcatc acgctctctt gcgcggacct
      601 attcctcgcc gaatttaagc ctccggaaca ccgcgcccag aatgttggaa tagtcgtcgg
      661 tccagacccg ctggccttcg gttggctcgg tctcggccca tttgtccgaa gaagcgaggt
      721 ttccgacatc ggcctcctcc cgcgcacaga ccacgacatc ggttgcgaag atatattcgt
      781 cgtcgcgcct ggaatcttcg tcatagaccc aactcttgag atcgttggca tcggcgatgc
      841 caaccaccac gctttccagc tctagatgcc ggtttgagac gtgcatcacg accgcgccct
      901 gcggggccag cttggacttg tagatcttca tcgcctcttc ggtggcaaga tgaatcggga
      961 tcgcatccga ggaataggcg tcgacgatga tgaggtcgta gaacccgtcg ggctccctgg
     1021 caaaggtcag cctggcgtcg ccgatcaccg gctttgcgtc cggctcgcag ctccgcatgt
     1081 aagtgaaata tttcggatcg cgcgcggtat cgaccatggt ctggtcgatc tcgaagaact
     1141 tccaggtctc gccaggctcg gaggcgcagg cgagcgtgcc ggcgccgacg ccgatgacgg
     1201 cgactttcag cgggccgccc ttccgttcgc ggatcgcggt gatcgcctgc ccgatgccgc
     1261 cgtccgtgtg atagtaggtg atcggctcgg gccggccttc gagcggagag ccgtcgttgt
     1321 tcaaaacttt ctgggcgccg tggatggtcg tgccgtgcat cagcacgtga tactgcccat
     1381 gcgaggtcat tacgatcttg tgcacaccga agaagctgcg cacggtttcg acccggccgt
     1441 catccgccgg atagaagcgg atcagaacca gcgccagcac gacagttgca aaaactttcc
     1501 agcggctcgc cttcagtgca agcgcgatca ggcctgcaag tacgccgacg ctaccggcca
     1561 cccagacgcg gcgcccctcg agccaggcaa acaccttgcc gccgccggca accgtcggcg
     1621 cgatcagcgc aattgcgagc gccgccagca agagccaata ccagcggctc cattgcggcc
     1681 agcgctcgct cgagggcggc cggcacaagg cggccagcgc cagcaggatc gggtattcgg
     1741 cgacccagga aaacgcgtat ggcgagatca ggccggcaaa cagtcccccc aacatgccgc
     1801 cgaacgacag cgcgacataa aagccggtga gatattttgc cggcgggcgg gtgcgcgcaa
     1861 gctcgccatg gcaggccatg gcgatgacga agaagcaaag ctgatgaccg ccgagcgtga
     1921 gcagcaagtt ttgttcgccg ccgaccgcga gcagcaccgc gacgcccgcg attgcgatcg
     1981 gctgcagcgt cagcatccac cggtgcggca acagcggtcg cgactggaac accagcaccc
     2041 aggtgaggag atagagcgac agcggcagca cccatagcaa cggcgcagcc gcgacgtcgg
     2101 tcgagatatg cgcggtgacc gcgataagaa gcccggacgg caccgcggca aggaagatcc
     2161 agcgcgcccg cagcacccag cctggcggca gcgcctgcgt gtcctcgcgc tgcagatcgg
     2221 cggcggcctt gggcgagcgc aacagcacca cgccgcagcc ggcgatcaaa agaatcaaca
     2281 gcccatagcc ggcggtccac atcaggttct gcgtgcgcag cgtgaacatc ggctcgagca
     2341 gaaccggata ggacaacagc gcgagaaagc tgccgatatt ggaggaggca tagaggaagt
     2401 atggatcggg cccggcagga tggcccgtcc gcacgaacca ggcctgcagc atggggttg
//
LOCUS       DATLJT010000788         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3955304,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000788 DATLJT010000000
VERSION     DATLJT010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3955304"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..679
                     /gene="rplD"
                     /locus_tag="VM910_17875"
     CDS             59..679
                     /gene="rplD"
                     /locus_tag="VM910_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HVI64429.1"
                     /translation="MEVQVTTLDGKKAGSVTLSDAIFGLEPRADIIHRCVVWQLAKRQ
                     RGTHEVKNRAEITRTGKKMYRQKGTGSARHGSARVNLFRGGGRSFGPHTRSHAIGLPK
                     KVRALALKHALSAKAKDGGILVIEALSVKEAKTKALRGHFDKLGLSNALFIDGTEVAA
                     DVRKAARNIPNIDVLPVAGINVYDIMRRNKLVLTRAALDALEARFK"
     gene            676..975
                     /locus_tag="VM910_17880"
     CDS             676..975
                     /locus_tag="VM910_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HVI64430.1"
                     /translation="MTTNDPRHYDIIVAPLITEKATNASEFNKVVFKVAMHATKPQIK
                     EAVEKLFDVKVKSVNTLRRPGKWRTFRGRYGRTSDTKRAVVTLEEGQTIDVTTGL"
     gene            986..1813
                     /gene="rplB"
                     /locus_tag="VM910_17885"
     CDS             986..1813
                     /gene="rplB"
                     /locus_tag="VM910_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HVI64431.1"
                     /translation="MALKTYNPVTAAQRQLVLIDRTGLHQGKPVKALTEGKNSTGGRN
                     NNGHITSRYRGGGHKQAYRKIDFKRCKFDVPAKIERFEYDPNRTGFIALVKYEDGELA
                     YILAPQRLSPGDTIVAGEHVDVKPGNAMPTANIPVGTIVHNIELKIGKGGQIARSAGA
                     YAQIVGRDGEYVILRLNSGEQRMVHGRCIATIGAVSNPDNMNVSIGKAGRTRWMGRRG
                     HVRGETMNPVDHPLGGRTRGGRHPVTPWGKPTKGMKTRKNKRTAKFIMASRHARKQK"
     gene            1822..>2124
                     /gene="rpsS"
                     /locus_tag="VM910_17890"
     CDS             1822..>2124
                     /gene="rpsS"
                     /locus_tag="VM910_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HVI64432.1"
                     /translation="MARSVWKGPFVDGYLLKKADAARSSGRHEMIRIWSRRSTILPQF
                     VGLTFGVYNGQKHIPVIVTEEMVGHKFGEFAPTRSFYGHSSDRKAKRATVPGAPGAA"
BASE COUNT          458 a          662 c          667 g          337 t
ORIGIN      
        1 gaagcaaagg gcgcaagcaa acctgccgcg ggcaagaagc cgaagaaggg cgtgtgatat
       61 ggaagttcaa gtcaccacgc tcgacggtaa gaaggctggc tcggtcacgc tgtccgacgc
      121 cattttcggg ctcgaaccgc gtgccgacat tattcatcgc tgcgtcgtct ggcagctggc
      181 gaaacgccag cgcgggacgc atgaggtcaa gaaccgggcg gagatcacgc gcaccggaaa
      241 aaagatgtac cgccagaagg gcactggctc ggcgcgccac ggctcggcgc gtgtcaattt
      301 attccgcggc ggcggccgct cgttcggacc gcatacacgc agtcatgcca tcggcttgcc
      361 gaagaaggtg cgggcgctgg cgctcaagca cgcgctgtcc gccaaagcta aggacggcgg
      421 cattctggtg atcgaggcgc tttccgtgaa ggaagccaag accaaggcgc tgcgcggcca
      481 tttcgacaag ctcggcctgt ccaatgcctt atttatcgac ggcaccgaag tcgcagccga
      541 tgtgcgcaag gcggcgcgca atattccgaa tatcgacgtg ctgccggtcg ccggcatcaa
      601 cgtctacgac attatgcggc gcaataagct cgtgcttacg cgggccgcgc tcgatgcgct
      661 ggaggcgcgg ttcaaatgac caccaacgat ccgcgccatt acgacattat cgttgcgccg
      721 ctcatcaccg agaaagcgac caacgcgtcc gaattcaaca aggtcgtctt caaagtcgcc
      781 atgcacgcga ccaagccgca gatcaaggaa gcggtcgaga agctgttcga cgtcaaggtc
      841 aagagcgtca acacgctgcg ccgccccggc aaatggagga cattccgcgg gcgttacggc
      901 cgcacctccg ataccaaacg cgcggtcgtg accctcgaag agggtcaaac gatcgacgtg
      961 accacggggc tgtgaggagc gggcgatggc attgaagacc tacaatccgg tcactgccgc
     1021 gcagcgccag ctggtgctga tcgaccgcac cgggctgcat cagggcaagc cggtcaaggc
     1081 gctcaccgaa ggcaagaact cgaccggcgg ccgcaacaat aacgggcaca tcacctcgcg
     1141 ttatcgcggc ggcggccaca aacaggccta tcgcaagatc gatttcaagc gctgcaagtt
     1201 cgatgtaccg gccaagatcg agcggttcga atatgatccg aaccgcaccg gcttcatcgc
     1261 gctggtcaaa tatgaggacg gcgagctcgc ctacattctg gcgccgcaac gcctctcccc
     1321 gggcgacact atcgtggccg gcgagcatgt cgacgtgaag ccaggtaacg ccatgccgac
     1381 cgcaaacatt ccggtcggca ccatcgtcca caatatcgaa ttgaagatcg gcaagggtgg
     1441 gcagatcgcc cgctcggccg gcgcctatgc gcagatcgtc ggccgcgacg gcgaatatgt
     1501 catcctgcgg ctcaattccg gcgagcagcg gatggtgcac ggccgctgca tcgccacgat
     1561 cggtgcggtg tcgaaccccg acaatatgaa cgtgtcgatc ggcaaggccg gacgtacccg
     1621 ctggatgggc cggcgcggcc atgtccgcgg cgagaccatg aatccggtcg atcatccgct
     1681 cggcgggcgc acccgcggcg gccggcatcc ggtcacgcca tggggtaagc cgaccaaggg
     1741 aatgaagacg cgcaagaaca agcgtacggc gaagttcatc atggcaagcc gtcacgcgcg
     1801 caagcagaag taggagacag tatggcgcgt tcggtctgga agggtccgtt tgtcgacggt
     1861 tatctgctca agaaggcgga tgccgcgcgc tcgtccggac gccacgagat gattcgcatc
     1921 tggtcgcggc gctcgacgat cctgccgcaa ttcgtcgggc tcaccttcgg cgtctataac
     1981 gggcagaagc acatcccggt gatcgtcacc gaggaaatgg tcggtcacaa attcggcgaa
     2041 ttcgcgccga cgcgctcgtt ctatggccat tcgtcggatc gcaaggctaa acgcgccact
     2101 gtcccaggcg cgcccggcgc cgcc
//
LOCUS       DATLJT010000789         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_509386,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000789 DATLJT010000000
VERSION     DATLJT010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509386"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VM910_17895"
     CDS             complement(<1..491)
                     /locus_tag="VM910_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI64433.1"
                     /translation="MLDRRELMKRAGMTALATGFGSARAFALETVTLPFDNGERPLVN
                     YPQKRPMIGQTSRPPQLETPFRIFDEGPITPNNAFFVRYHLSDLPFDIDPDKFTLEVK
                     GKVDHPLKLSLQEIRKLKAVELVAVNQCSGNSRGFFNPRVAGGQLGNGAMGNARWRGV
                     PLKT"
     gene            complement(644..2383)
                     /locus_tag="VM910_17900"
     CDS             complement(644..2383)
                     /locus_tag="VM910_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI64434.1"
                     /translation="MVLFSRDQIRKALSGIGVRQVRLASGLVLFTYLVSHFLNHALGN
                     ISVEALTEGVHYHTLFWQFLPVAIILYTAVLVHFSLGIWALYQRRQFAWRTIELLQLL
                     LGLSIPLLIASHIIGARLGETLFGHEKLYPQELYAFWVARPIRAWQMSIVLVIAWMHG
                     CIGLHFWLRMKAFYAKLAPYLLAAAVLVPTLALLGFYQGGQSVIADSGDAEWRAKNLA
                     PNQVGTPAEQQTLETIIDDFLTFYLVLLGAVVLARGARLLIERRGGMIALSYGNGKTI
                     RVPKGLSVLEASLRYNVPHASVCGGRARCSTCRIRIIGDCSSLPEPSPREAFVLARVG
                     TSDPSIRLACQLRPTADLSFFQLFLPNTSTANEHASNPTRIGQERYLVSLFVDMRGST
                     RLAEKMLPFDTVFVVNRFLSAVAQAVTESGGRPNQFVGDGMLALFGLASSAQAACRQA
                     LKAAALIAKNVDDVNQFLSHDLREPIRFGIGIHGGEVIVGDIGYRDHMVFTALGDAVN
                     VAARLQDMTKTLACEAIVSDEVRITAGLGADHLPKQEVTIRGRAEPMLVSVVADARAL
                     SALVTSSEAAVAA"
     gene            2632..>2855
                     /locus_tag="VM910_17905"
     CDS             2632..>2855
                     /locus_tag="VM910_17905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVI64435.1"
                     /translation="MTALRIAFAQLMELRTDEAYYWTWSKEHALSFLDHPPMIAWFIR
                     FGTAIFGDTAFGARFAGILAMLMTQLLLAD"
BASE COUNT          554 a          878 c          906 g          517 t
ORIGIN      
        1 gtcttcagcg gcacgccgcg ccagcgcgcg ttccccatcg cgccgttgcc gagctggccg
       61 ccggccacgc gcggattgaa gaagccgcgg ctgttgcccg agcattgatt gacggcgacg
      121 agttcgaccg ccttcagctt cctgatctcc tgcagcgaca gtttgagcgg atggtcgacc
      181 ttgcccttga cctcgagcgt gaacttgtcg gggtcgatat caaacggcag gtcggacaga
      241 tgatagcgta cgaaaaaggc gttgttcggc gtgatcgggc cctcgtcgaa gatgcggaac
      301 ggcgtctcca gttgcggggg acgactggtc tgaccgatca tggggcgctt ttgcgggtaa
      361 ttcaccagcg gtcgctcgcc attgtcgaac ggcagtgtca cggtttcgag cgcgaacgct
      421 cttgccgatc cgaaacccgt tgccagcgcg gtcatgccgg cgcgcttcat caactcccgc
      481 ctgtcgagca ttttgaaact tcctcccaag cattttctgt gagccagtcc gttgcgcgga
      541 tcaggacagc ctgagtgttg cgagcgccgc tacccaaagt caaatcctgc tttcgacgca
      601 ctcgctgcga tgcggcaagg caaaacaagc ggggattttt acgtcacgcg gcgacggcgg
      661 cctcagagct cgtgacaagc gccgacagcg ccctggcatc ggcgaccaca ctgacgagca
      721 tcggctcggc gcgcccgcga atcgtgacct cctgcttcgg cagatggtct gcccccagcc
      781 ctgcggtaat gcgcacctcg tcggagacga tggcctcgca ggcgagagtc ttcgtcatgt
      841 cctgcaacct cgcagcgaca ttgacggcat cgccaagcgc ggtgaacacc atgtggtcgc
      901 gatagccgat gtcgccgacg atcacctcgc cgccgtgaat gccgatgccg aagcggatcg
      961 gctcgcgcaa atcgtggctc aagaactggt tcacgtcgtc gacatttttc gcgatcaggg
     1021 ctgcggcttt cagagcctgg cggcatgcgg cctgcgcgct cgatgcaagt ccgaacagcg
     1081 ccagcatgcc gtcgccgacg aattgattgg gcctgccacc gctttcggtc accgcctgcg
     1141 ccaccgcgga caggaagcga ttgacgacga agacggtgtc gaacggcagc atcttttcag
     1201 cgagccgggt cgagccgcgc atgtccacga acaggctgac gagataacgc tcctgaccga
     1261 tccgggtcgg gttcgaggca tgttcgtttg ccgtcgacgt gttgggaagg aagagctgga
     1321 agaacgacag gtccgcggtt gggcgaagct ggcaggcaag ccggatcgaa gggtcgctag
     1381 tgccgacgcg ggcgagcacg aaggcctcgc gcggcgacgg ctccgggagc gagctgcaat
     1441 ccccgatgat gcgaatacgg caggtcgagc agcgcgcgcg tccgccgcac acgctggcat
     1501 gcgggacatt gtaacgcagg ctcgcttcca gcacgctgag ccccttgggc acgcgaatcg
     1561 ttttgccgtt gccgtaagaa agcgcaatca taccgccacg gcgttcgatc agaaggcggg
     1621 cgccgcgcgc cagcacgacg gctccgagca ggacgaggta gaaggtcagg aaatcgtcga
     1681 tgatggtctc gagcgtctgc tgctcggccg gcgtgccgac ctggttcggc gcgagatttt
     1741 tggcccgcca ctccgcgtcc ccgctgtcgg caatgacgct ctgtccgccc tggtagaagc
     1801 cgagcagcgc aagcgtcgga accagtaccg cggcagcgag cagataaggt gcaagcttcg
     1861 cgtagaatgc cttcatgcgc agccagaaat gcaggccgat gcagccatgc atccaggcga
     1921 tgaccagaac gatcgacatc tgccacgccc tgatcggccg cgccacccag aatgcataga
     1981 gttcctgcgg gtacaatttc tcgtggccaa acagcgtctc gccgagccgg gcgccgatga
     2041 tgtggctcgc gatcagcagc ggaatgctca agccgagcag gagctgcagc aattcgatgg
     2101 tgcgccaggc aaattgccgg cgctggtaga gtgcccagat cccaagactg aaatgtacca
     2161 gcaccgcggt atagagaatg atcgcaaccg gaaggaactg ccagaacagg gtgtgatagt
     2221 gcacgccctc ggtaagcgct tccaccgaga tattgccgag cgcatggttg aggaaatggc
     2281 tgaccagata ggtgaacaac acgagcccgg aggcgaggcg gacctgccga acgccgatgc
     2341 cactgagggc tttcctgatc tgatcgcgcg aaaataggac catttgccga tcattatcga
     2401 ccataggggg cattcattcc acgctaaatg catcaccaat gccttaccgc ggtgattggc
     2461 gcgggtccgg tcggcagggc agggcgaggg ggccttcagc tcccggtcgg tacacgttga
     2521 ctccccgaag ggagtcgggg agaaagcgcc gtgccttcga cctcccagcc cgcaaagccg
     2581 ggcgatttcg ccgttcgacc gctgattttc gccgccatcg tgattgccgc gatgacggcg
     2641 ttgcggatcg ctttcgctca actgatggag ttgcgcaccg acgaggccta ttactggacc
     2701 tggtcgaagg agcacgcgct gtcgttcctc gatcatccgc cgatgatcgc ctggttcatc
     2761 cgcttcggca ctgcgatttt cggcgacacc gctttcggtg ccagattcgc cggcatcctg
     2821 gcgatgctga tgacccaatt gctgctggca gacat
//
LOCUS       DATLJT010000790         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1658310,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000790 DATLJT010000000
VERSION     DATLJT010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658310"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VM910_17910"
     CDS             complement(<1..280)
                     /locus_tag="VM910_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVI64436.1"
                     /translation="MAFAISQTAQPRLALPQGTTTAPPAGATTTINPRTRLLLEGPIA
                     ATLLRLAPPNLVVNVILIAVTASIDAHFVGRLGAPALAGVSLVFPLMML"
     gene            complement(356..625)
                     /locus_tag="VM910_17915"
     CDS             complement(356..625)
                     /locus_tag="VM910_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64437.1"
                     /translation="MLRNCDDTLGFDPIHRERNLTVWSSPPPAVSAVRKMGAAFCEGL
                     AAHREYEALRSRGIAHERALREALGLGCARLPRETVTPLYFAGRA"
     gene            907..2061
                     /locus_tag="VM910_17920"
     CDS             907..2061
                     /locus_tag="VM910_17920"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HVI64438.1"
                     /translation="MSSFAIEKAAGQAPGSPTQPTIEPSTIKRRSVGLRGYDPERAFT
                     GFTLFSPLPTTNKTVYLVDMLGNVVHTWNMPYPPGQSGYLTERGTLFYNGQIPNQSHV
                     GKAPYRGGAALEMDWSGRILWEVNHPDHNHDGIRLRNGNILLICQKPLPAEIVAKVRG
                     GRPGSEYDDGKMDAPYLVEMTIKGQIVWEWRSWEHLNPENDVITAVQDDRDVWTVANA
                     VSEMPDGNLLVSFRDISTVVMINRRTGAIYWKLGAPPLSGQHAPHLLSNGNVLLFDNG
                     PHRLDHSFPFSRVLEIDPATKAIVWSYHEVRVSDFFSPRISNAQRLPNGNTFINEGWF
                     GRFFEVTREGSVVWEYVNPYFGSRQQLVINAVQRAYRYSAAEIARAKSAT"
     gene            complement(2154..3092)
                     /locus_tag="VM910_17925"
     CDS             complement(2154..3092)
                     /locus_tag="VM910_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVI64439.1"
                     /translation="MPRRLPPLNALKAFEAAARHESFTRAAEELCVTQGAVSHQVKAL
                     EAELGLKLFNRERQQLVITEAGRAYLVVVRDAFDRIGDGTERLLQRQRGGALTVSTSP
                     NFAAKWLVHRLSRFAETHPEIDLRVSASMHHVDFAREDIDLAIRHGDGTDSGLHVTRL
                     CTEEMFPVCSPKLLNGRNRLRTPSDLNRFALLHVNDRQGWRQWLDFADVGDVDAARGP
                     ILNQASMAIDAAVDGQGVALARTALAAWDLIGGRLVRPFEIAMPVSFAYWIVCSKAVA
                     KLPKIVAFNDWLLAEAAADARQLEGLKSRQPARASR"
     gene            3337..3477
                     /gene="prfB"
                     /locus_tag="VM910_17930"
                     /pseudo
     CDS             3337..3477
                     /gene="prfB"
                     /locus_tag="VM910_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146545666.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          678 a         1067 c         1103 g          629 t
ORIGIN      
        1 tcaacatcat gaggggaaac accagcgaca cgccggcaag cgcgggcgcg cccaggcggc
       61 cgacgaaatg cgcgtcgatg ctggcggtga ccgcgatcag gatgacgttg acgaccaggt
      121 tgggcggcgc gagccgcagc aaggtcgccg cgatcggtcc ctcgagcagc agccgcgtgc
      181 gcgggttgat cgtcgtcgtg gcgccagccg gaggcgctgt cgtcgtgccc tgcggcaggg
      241 cgagtctggg ttgtgcggtt tgcgaaatgg cgaatgccat gggtgtctcc catctcgatc
      301 aaggcaggac aatctgcgct cggccaatta ggttgaatgc ggcatcgatc ggcgctcatg
      361 cccttccggc gaaatacaaa ggcgtgaccg tttcccgcgg cagccgcgcg cagccgaggc
      421 cgagcgcttc ccgcagcgcc cgttcgtgcg caatgccccg cgacctcagc gcctcgtact
      481 cgcgatgcgc ggcgagacct tcgcaaaagg ccgcacccat cttccgcacg gcggaaacgg
      541 cgggcggcgg cgaggaccaa acggtaagat tccgctcccg atggatcgga tcgaacccca
      601 gtgtatcgtc acagttccgt aacatttttc atgctcccgc atatcaaggg acaggaccca
      661 tgtcccgtcc tggagccatg caaatagggc atcctgtacc ccggccgcaa agcatcgttt
      721 ctcgcgccgc ccatagaatt tctcatacat cccggtcttg aattgcggtt agatgataca
      781 tatgttacat gacaactgcg tacattgata catcatatac atggatgcat acacatggat
      841 gccgggcgtc gcggacttat tcggctggcc ggtgcggcgg cagcgtcggg aatgttgggg
      901 acgaacatgt ccagctttgc gattgagaag gcggccgggc aggctccagg ttccccgacc
      961 cagccaacga tcgagccgtc aacgatcaag cggcgcagcg tcggcttgcg cggctacgac
     1021 cccgagcgcg ccttcacggg cttcacgctg tttagtccgc tgccgaccac caacaaaact
     1081 gtgtacctgg tcgatatgct cggcaacgtc gtccacacgt ggaatatgcc ctacccaccc
     1141 ggtcaatccg gatatctcac cgagcgtggc acgctcttct acaacggcca gattccgaac
     1201 cagagccacg ttgggaaggc tccctatcgg ggcggtgccg cgttggaaat ggattggagc
     1261 ggacggattt tgtgggaggt gaaccatccc gatcacaatc acgatggcat tcgcctgaga
     1321 aatggaaata ttcttttgat ctgccagaag ccgcttcccg ccgagattgt tgccaaggtg
     1381 cggggtggcc ggcccggctc ggagtacgac gatggaaaga tggacgcgcc gtacctcgtc
     1441 gagatgacga tcaaaggaca aattgtatgg gaatggcgaa gctgggagca cttgaatccc
     1501 gagaacgacg tgatcacggc cgtccaggat gatcgcgacg tgtggaccgt cgccaacgca
     1561 gtctccgaga tgcccgacgg aaacttgctt gtgagtttcc gcgacatctc cacggtcgtg
     1621 atgatcaacc gccgaaccgg cgccatctat tggaagctcg gcgcaccccc tctttcgggc
     1681 cagcacgcgc cgcaccttct gtcgaacgga aacgtgctgc tcttcgataa cgggccgcat
     1741 cgactggacc actcgttccc attctcgcgt gtgctcgaaa tcgatccggc gacaaaagcg
     1801 atcgtgtgga gctatcacga ggttcgggtg tcggatttct tcagtcccag aatctcgaac
     1861 gcccagcgct tgccgaacgg caacacgttc atcaacgagg gctggtttgg ccggttcttc
     1921 gaggtcaccc gcgagggctc tgtggtctgg gagtacgtca atccgtattt tggctcgcgc
     1981 cagcaactcg tgatcaatgc cgtgcagcgt gcctaccgct attccgccgc ggaaatcgct
     2041 cgggccaaat ctgcgactta atcactcgac cccgctggga ggcacgccaa ggtccctgca
     2101 caaaccttga ccgcatgccg ccatcaagca gccggtgagg ccagggaaag aaatcagcgc
     2161 gaagctcgtg caggttgcct cgatttcagt ccctcgagtt gccgggcatc cgcggcggct
     2221 tccgccagaa gccaatcgtt gaatgccacg attttcggca gcttggcgac cgccttcgag
     2281 cagacgatcc agtacgcgaa ggacaccggc attgcgatct cgaacggccg cacgaggcgg
     2341 ccgccgatca gatcccaggc cgcaagcgcc gtgcgggcga gcgccacacc ctggccgtcg
     2401 accgctgcat cgatcgccat gctggcttga ttgagaatgg ggccgcgtgc ggcgtcgacg
     2461 tcgccaacgt cggcaaagtc gagccattgg cgccaccctt ggcggtcgtt gacgtgcaaa
     2521 agcgcgaagc ggttgagatc ggagggcgtg cgcaggcggt tgcggccgtt gagaagcttg
     2581 gggctgcaga ccggaaacat ctcctccgtg cagagccggg tcacgtgcag gccggagtcg
     2641 gttccgtcgc cgtggcggat ggctagatcg atgtcctcgc gcgcgaagtc gacgtggtgc
     2701 atcgacgcgc tgacccgcag atcaatctcg gggtgggtct cggcgaaacg gctgagccgg
     2761 tggacgagcc acttcgccgc gaagttcggc gaggtgctca cggtcagggc gccgccgcgc
     2821 tggcgttgca gcaatcgctc ggttccgtcg ccgatgcggt cgaaagcatc gcgcacgacc
     2881 acgagatagg cgcggcctgc ctcggtgatc accagctgtt gccgttcgcg attgaacagc
     2941 ttcaacccga gctcggcctc gagtgccttc acctgatggc tgaccgcgcc ttgggtgacg
     3001 cagagttctt cggcggcgcg cgtgaagctc tcgtggcggg ccgcggcctc gaaagccttg
     3061 agcgcgttga gcggcggcag gcggcgcggc atgggcgcac caaatcatga gaaaaactga
     3121 ggcaggctaa agaaaacagc ctttacctgc aacgaaattt aggctctgcg ctgcggcaaa
     3181 ttcatcatct gtgcgttcgg gccgagcccg ggcggagccg cttcgcatcg aacggtatct
     3241 tcgttggcgc gacgttcggc gcccattgcg tcgatcgcgt gcagtggcta aatcccgcgg
     3301 gcgggcgacg cgtcgagcac gcctcgcggg gagaccatgc gcgcggaagt tcaaaatatc
     3361 gtcgacgaga tcaagcagtc ggtcggactg ctgaggaggc atctttgact tcgacaaggc
     3421 gcgcgcgcgc ctggcggagc tcaacaagca ggccgaagac cccacgctct ggaacga
//
LOCUS       DATLJT010000791         7271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1658385,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000791 DATLJT010000000
VERSION     DATLJT010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658385"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1419..2483)
                     /locus_tag="VM910_17935"
     CDS             complement(1419..2483)
                     /locus_tag="VM910_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64440.1"
                     /translation="MAKVEFRHVVKRFGDAEVIPDLHVTIEDGEFVALLGPSGCGKST
                     SLLILAGIYLPSGGELLFDGRIVNEVEAKDRNVGIVFQSYALYPHMTVRDNVLFPLRF
                     KRMPREEALGRVMTAANLVQVEGLLDRRPGDLSGGQQQRVALARALVKEPQLLLLDEP
                     LSNLDATLRLTMRTEIKSLQKKLGVTTVLVTHDQIEATTMADRIICMRAGRIEQIGTP
                     DDLYRRPQSLFIAGFIGSPPINMVNGEARDGAVRVGPVAFPFAGAPGPVTVGLRPEHL
                     RFTGSGLSGQIAQMEPMGREILYVVDTAVGYLRVLEHGSVAAHAVGEPVNIGFLPEDS
                     LVFDTASERLVTAARVSPPA"
     gene            complement(2489..3589)
                     /locus_tag="VM910_17940"
     CDS             complement(2489..3589)
                     /locus_tag="VM910_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVI64441.1"
                     /translation="MAAPLTFTGIHSRRTTPVDWDRMAAGARALRRTKATALYAFLIS
                     VSLPIILPYFWLVTIAFSARTGVADTFVLWRAMAVLAPAVIAFWLATTLARNRRQLWL
                     GVSAIALIVTVVFALLVGPDLHLRNFIFLVEPDFASVVRSRVGEVRGAVQFPSVWRAF
                     GNSMLIAGSQTVIVLMVASLAAYYLSRFQFPGRAAMLRSLLVLHAFPALTLIVPIFLM
                     LYWIGLLDTLLGVILVLVAFELPFAIFIMKGFFDAVPWDIEMSALTDGASRRQAFLWV
                     VLPQVRNGLLAIAVFTFIRGWEEYIFVFTFLIRSTNWTMSLYMYYVRDDVMGVDYGVV
                     SAVGVFYLVPSLILYLAAQRYLMQMSIGGVKG"
     gene            complement(3591..4520)
                     /locus_tag="VM910_17945"
     CDS             complement(3591..4520)
                     /locus_tag="VM910_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI64442.1"
                     /translation="MVERRDRASSAARRRINFAIFLGPAIALTVLFFFVPALIDVAVS
                     FTDLDRTLRLTKFTAEQYQKVLEGDRRLPQVIGLTFVYAFGTLAIFNLTFGLVLALTT
                     TSLNKISGGFFRGIWLLPRMSPSVLYILLWLWVINPSDVGLLNQFLIQVLGLTKPLDL
                     MTSAPVVLIIIANGFIGASLGMIIFTAAIRGIPEHLFYAAAVDGAGTLAPIRFITLPA
                     LRWPISFVTIYQTLSLLVSFEYILLLTKGGPFYDTTVYALYVYRRAFTSGQYGYGAAL
                     ALFLIVIGAIVALLAWRFFDLKRLLQRPRIEVH"
     gene            complement(4549..5958)
                     /locus_tag="VM910_17950"
     CDS             complement(4549..5958)
                     /locus_tag="VM910_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI64443.1"
                     /translation="MVLDVSEMKAATMRLWKAAGATLLIVTVAVSSSVIFASAPVLAQ
                     NTVKLELWSRQDPSGPLRPANVVKAADRLNKELGAEGSGKRVEVAVHESPAPGFDDDA
                     LQLLKVFGIGQGPDMFIAAHEWICAFQQEGFALKLDDYIAKYPQHFGTIFPSLWQSVK
                     CSDGIYGIPQDAEARMFFYNKKLLREAGYDDAFIEAMPARTLSGELTMDDVIDIGKRV
                     ISKTKAQYGILHRPNKGPDYIMAFQSYGNNFADSGTGKLLFERDKLAAAFGWFERGVK
                     QGVIPANNTAMEFDALRKAFYTGNAAFWMYGIWDLGTYAFPTFGLPKDEAGFFADWGW
                     IAAPAAKKGGQAGSLTHPIIYAVAAHVAEPELAVRLLGHASDADLNTDHAVSTTHLGI
                     RSEQLKDPRYAEAWPLARATELLKITKFLPNNPQFGDLNGILYTALQGVETGRLSAKQ
                     AADFVIDEATASLKDVIVK"
     gene            6059..6871
                     /locus_tag="VM910_17955"
     CDS             6059..6871
                     /locus_tag="VM910_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVI64444.1"
                     /translation="MPFSFLAREAGRVHVCGHRGYSLRYPENTLPSFGAAKAWGATTI
                     EIDVVLTAEGEPIVLHDRTLDRTTDGHGFVADLGLEQIRSLDAGARFDARFAGTRVPT
                     VAEIVDWAKREEMGIFLEIKEAEQPDLAVDRVARLLEATGAADRVLVISLDHVVLKRG
                     VERHPGIRTQVITHARHADIIGVLRGCGARSVSIEFDMFHAKDAKALHDAGFSNRVSL
                     PGVGVLAEYWRGGRDIIPSIVQWIAEGLIDTISGDDVPFLARLVERAGRADR"
     gene            6957..7271
                     /locus_tag="VM910_17960"
                     /pseudo
     CDS             6957..7271
                     /locus_tag="VM910_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
BASE COUNT         1492 a         2220 c         2223 g         1336 t
ORIGIN      
        1 gtccgcttct ataacgagcg ccgaccgcac caggccttgg gttacaagac tccggcggcg
       61 acctgggcgg ccgaggtgag ccctgtggat ttgccgctgc gcttggacga cgctggcgcg
      121 ccgcccacaa ctccacagga ccaacaacag caaaacgtga tacacatttg acgaggaaga
      181 ggagcggcct tcacctttgc ccaatctcca ggtccctcac tacttcctgc gtctgtcttg
      241 aaccgaggcc gcttccctcc accggcatta cccggcttcc tcggtactgc gggcctctcc
      301 gccaccccag cgcgcccggc ctgtccctca cgggcgtccg gttgatcatc cctgatcacg
      361 ccttggggct tctcgtgttg cgtgcgcttt ccttgtgtac atgctgccgc cactaccccg
      421 gtgccgtgga tgggcgcact cgtcgctcaa acttggccat ccatatcaac cttccccgaa
      481 accactatcg ggtcggcctg cacatcgacc ttttcgaggc ttgctcggcg tttactcgcg
      541 ttgcggcctg ctcactcgcg ctgtcaccaa ttcgtgacac gctaatcgaa ggcttcagcc
      601 atttcgttac ctccatgact gctccgattg cttccggctg gagcgaaacg ccgggtgggc
      661 cttgcaccca ctggaaagcg ccgccttgtc acggcgcacg tggaaagcgg ccatccaggc
      721 ggtgcgaaaa tcaagagttt ttgcacgctc tctcttaagg ttgcctttgc aagtcagatc
      781 gttgttttgc aagcgctcta ttccatgagg aactacggcc cacccgcaca tattctcctc
      841 ccagatcgaa attctcggca gtgggagagg cttgtcctcg aataaagccg ctgcaatgcc
      901 atagagggtg agcaagagat ccagttccca gtagactgtt gtcccgcacg tcggacaaaa
      961 gtaatttcgg atttttcgac ttcctcgtgc ctcgcgttca tagcaacaac ctgtgccttc
     1021 gagctgaacc tgagttcgtt caaatcggac attgtaactg aacgccgcgc ccgttcggcc
     1081 ttgacagtct atacaatggc agataatcac acgcacgggt tcgcctgtga ctttggctcg
     1141 cagagctccg cattgacatc tcgtcgtcct gtccatagcc ctgttctcta tttgcaggtc
     1201 tgactagcaa gccacgatag acatgggcgg tcaacaaaag aagagggtaa gatgatgcgg
     1261 gattcaagga tttcgcaccc gcggacgatc ctccgggaat tcaatggggt tagccggtgc
     1321 aaaattcggg tggaatgacc aaaaagggtc gaatttcccg ttcagtgacg agagcgggaa
     1381 ggtccggtgt tggcgcctct cgtcggttgg cccgcccatc acgccggcgg gctcacccgc
     1441 gcggcggtca ccagtctttc gctggcggtg tcgaacacca gtgagtcctc cggcaagaag
     1501 ccgatattga ccggttcgcc gacggcgtgc gccgcgaccg agccgtgctc gagcacccgc
     1561 aaatatccga cagcggtgtc gacgacataa aggatctcgc gacccatcgg ctccatctgg
     1621 gcgatctgcc cgctcaagcc cgagccggtg aatctcaggt gctccgggcg cagtccgacg
     1681 gtcacgggac cgggtgcgcc cgcgaacgga aacgcgaccg gcccgacccg caccgccccg
     1741 tccctcgcct cgccattgac catgttgatc ggcggcgatc cgatgaagcc ggcgatgaac
     1801 aggctctgcg gcctgcggta aagatcgtcc ggcgtgccga tctgctcgat ccggccggca
     1861 cgcatgcaga tgatgcggtc ggccatggtc gtcgcctcga tctggtcgtg ggtaacgagt
     1921 accgtcgtca caccgagctt tttctgcagc gatttgatct cggtgcgcat cgtcagccgg
     1981 agcgtcgcgt cgagattgga aagcggctcg tcgaggagca ggagttgcgg ctctttgacc
     2041 agcgcgcgtg cgagcgccac ccgctgctgc tggccgccag acaagtcgcc cggccgccgg
     2101 tcgagaagcc cttcgacctg gacgagattg gccgccgtca tgacgcggcc gagcgcctcc
     2161 tcgcgcggca tccgcttgaa gcgaagcggg aacaagacgt tgtcccgcac ggtcatgtgt
     2221 gggtagagcg catatgactg gaagacgatg ccgacgttgc ggtccttggc ctcgacctcg
     2281 ttgacgatac ggccatcgaa taagagctcg ccgccgctcg gcagatagat accagccaga
     2341 atcaagaggc ttgtcgactt gccgcagccg gaagggccaa gaagtgctac gaactcgccg
     2401 tcctcgatcg tgacgtgaag atcagggatg acctcggcat ctccgaaccg cttgactacg
     2461 tggcggaact cgactttcgc catcggcgtc agccttttac gccgccgata gacatctgca
     2521 tcaggtaccg ctgcgcggcg agatagagga tgaggctcgg gacgaggtag aacaccccaa
     2581 ccgcggagac cacaccgtag tcgacgccca taacgtcgtc gcggacgtag tacatgtaga
     2641 ggctcatcgt ccagttggtg ctccggatca ggaacgtgaa gacgaagatg tactcctccc
     2701 atccacggat gaacgtgaag acggcgattg cgagaaggcc gtttctgacc tgcggcaaca
     2761 ccacccacag aaaggcctgg cgccgcgacg cgccgtcggt cagcgcgctc atttcgatgt
     2821 cccatggcac ggcgtcgaaa aagcccttca tgatgaagat cgcaaagggc agctcgaagg
     2881 cgacgagcac gagaatgacg ccgagaagcg tgtccaggag cccaatccaa tacagcatca
     2941 gaaagatcgg cacgatcaga gtcagcgccg gaaatgcgtg cagtaccagc agagagcgta
     3001 gcatcgcggc gcggccaggg aactggaatc gcgacaggta gtaggctgcg agcgaagcta
     3061 ccataaggac gatgacggtc tgcgacccgg cgatcagcat cgaattgccg aaggctctcc
     3121 agacgctcgg gaactgaacc gcgccgcgta cttccccgac acgagagcgc acgaccgagg
     3181 cgaaatccgg ttccaccaga aagatgaaat tgcgaaggtg caggtccggg cctaccagca
     3241 aggcgaagac taccgttaca atcagcgcaa ttgccgatac gccgagccac aactggcggc
     3301 ggttccgtgc caacgtcgtc gccagccaaa aggctatgac ggcaggcgcg agcacggcca
     3361 tcgcgcgcca taaaacgaat gtgtcggcga cgccggttct cgcggagaag gcgatcgtga
     3421 caagccagaa gtagggcaag atgatcggca gcgagacaga aatcaggaag gcgtacagag
     3481 cggtcgcctt ggtgcggcga agcgcgcggg cccccgcggc cattctgtcc caatccacag
     3541 gcgtggtccg cctggagtga attccggtaa acgtcagcgg cgccgccatc tcagtgcacc
     3601 tcgatccgag gacgctgcaa cagccgcttc aagtcgaaga agcgccaggc gagcagtgcg
     3661 acaatggcgc cgatgacaat gagaaaaagc gccagcgcag cgccatagcc gtattgcccg
     3721 ctcgtaaatg ctcgtcgata gacgtaaagc gcgtagacgg tcgtgtcgta gaacgggcca
     3781 cctttggtga gaagaagaat gtattcgaag ctcaccaaca gcgagagcgt ttggtagatc
     3841 gtcacaaaac tgatcggcca gcgcagcgca ggaagcgtaa tgaagcggat cggcgccagg
     3901 gtgcccgcgc catcgaccgc ggcggcgtag aacaggtgct cgggaatgcc ccgtatcgcc
     3961 gcggtgaaga taatcatgcc cagcgatgcg ccgataaaac cattggcgat gatgatcagc
     4021 acgaccggag cgctggtcat cagatcgagc ggctttgtca gaccaagcac ctgtatcagg
     4081 aactggttca acagccccac atccgagggg ttgatgaccc aaagccagag cagaatatag
     4141 aggaccgagg ggctcatgcg cggcaagagc cagatgccgc gaaaaaagcc gccggatatc
     4201 ttgttgagcg acgtggtggt gagcgcgagc acgagcccga atgtcaagtt gaagatggcg
     4261 agcgtgccaa aggcgtagac gaatgtcagg ccgatgacct gcgggagacg ccggtcgcct
     4321 tcaagcactt tctgatattg ctccgcggtg aatttggtga ggcgaagagt gcggtcgaga
     4381 tcggtgaacg acaccgcgac gtcgataagc gcggggacga agaagaagag aacagtgagg
     4441 gcgatcgcgg ggccaagaaa aatcgcaaag ttgatgcgcc tgcgagccgc agagctcgcg
     4501 cgatcgcggc gttcgaccat caactcgttc gttgccgcca ttgcgtcact atttgacgat
     4561 tacatccttg agagacgcgg ttgcctcgtc gatgacgaag tcggccgcct gctttgcgga
     4621 caggcggccc gtctcaaccc cctggagggc ggtgtagagg atgccgttga gatcaccgaa
     4681 ctgcgggtta ttgggtagaa atttggtgat cttcagaagt tccgtcgcgc gggcaagcgg
     4741 ccaggcctcg gcgtatcgcg gatctttgag ctgttcggac ctgatgccga gatgcgtcgt
     4801 actaaccgcg tggtcggtgt tgagatcggc gtccgaagcg tggccgagaa gccgcaccgc
     4861 gagctccggc tccgccacat gggcggcgac agcatagatg atcggatggg tcaggctacc
     4921 cgcttgcccg cccttttttg cggctggtgc ggctatccat ccccaatcgg caaagaaccc
     4981 ggcctcatcc tttggtagcc cgaaagtcgg aaacgcatag gtcccgagat cccagatgcc
     5041 atacatccag aacgctgcat ttcccgtgta gaacgccttg cgaagtgcgt caaactccat
     5101 cgcggtgttg ttggctggaa ttaccccctg cttgacgccg cgctcgaacc agccgaacgc
     5161 cgccgcaagc ttatcgcgtt cgaagaggag cttgccggtg ccggaatcgg cgaaattatt
     5221 gccgtatgac tggaacgcca taatgtagtc cggtccctta ttgggccggt gcaagatgcc
     5281 gtactgggcc ttggttttgc tgattacccg cttgccgata tcgatgacgt cgtccatcgt
     5341 cagctcaccc gacagcgttc gcgccggcat cgcctcgatg aaagcatcat cgtaaccggc
     5401 ctcgcgcaga agcttcttgt tgtagaagaa catgcgcgct tcggcgtcct gcgggatgcc
     5461 gtagatgccg tcggagcatt ttaccgactg ccagagcgac ggaaagatcg tgccgaaatg
     5521 ctgagggtat ttggcgatat aatcgtcgag tttgagcgca aagccttcct gttggaaggc
     5581 gcagatccat tcatgcgcgg cgatgaacat atccgggccc tggccgatgc cgaacacctt
     5641 gaggagctgt agggcatcat cgtcgaaccc cggcgccggg ctttcgtgga cggcaacctc
     5701 gacgcgcttg ccggagcctt cggctccaag ctccttgttc agccggtccg ccgccttcac
     5761 tacattggcc ggacgcaacg gacccgacgg gtcctggcgc gaccacagct caagcttgac
     5821 ggtattctgt gccagcacag gtgccgacgc gaagattacc gaagagctga ccgccacagt
     5881 gacaatcagt agagtcgcac cagccgcctt ccagagtcgc atcgtggccg ccttcatttc
     5941 cgacacgtct aacaccatat catctatccc ggtaatgatc gtcgtgtcgg cctgcggagc
     6001 aagttgaatc ttcgcccgct gatcattgca cccgcgcaaa agaggaatcc gtacgaccat
     6061 gcctttcagt ttcctggcgc gcgaggccgg gcgcgtccat gtgtgtggcc atcgcggcta
     6121 cagccttcgt tacccggaga acacgctgcc ctcattcgga gcggcgaagg cctggggcgc
     6181 gacgacgatc gagatcgacg tcgtactgac cgccgagggt gagccgatcg tgcttcatga
     6241 ccgcacgctc gaccgcacca ctgacggtca cggcttcgtc gcggacctcg ggctggagca
     6301 gatccgcagt ctcgacgccg gagcccgctt tgatgctcga tttgccggta cgcgcgttcc
     6361 gacagttgcg gagatcgtcg attgggcgaa gcgcgaggaa atggggatct ttctcgagat
     6421 caaggaggcc gagcagcccg acctcgccgt cgaccgagta gcgcgtttgc tcgaagcgac
     6481 gggcgcggcc gacagagttc ttgtcatcag cctcgaccac gtcgtgctga agcgcggggt
     6541 cgagcgccac ccggggatca ggacccaggt catcacgcac gcccgtcacg ccgacattat
     6601 cggtgtgttg cggggttgcg gcgccagatc cgtctcgatc gagttcgaca tgtttcacgc
     6661 gaaagacgcg aaagccctgc acgatgccgg gttttccaat cgggtcagtc tgccgggggt
     6721 cggggtccta gcagaatatt ggcggggtgg gcgcgacatc atcccctcca ttgtccaatg
     6781 gatcgccgag ggcctcatcg acacaatctc cggcgacgac gttccgttcc tggcccggct
     6841 cgtcgagcgt gccgggcggg cagatcgata gtagcggcct tggctcggtc ggatccaacc
     6901 gatctcgaca accgcctgcg ggtggccgag gccgtgggcc cgcgtccacg gccggccccc
     6961 caccggcctg gacgggattc cggccgctgc tcgtcgcgcg caagaaccgc gagagcagca
     7021 atgtgacatc actgatcctc gagcccgccg acggacagcc tctgcaggct gctttacccg
     7081 ggcagttcat cgtgctgcgg ctaaagccgg cacctgacgc gcccgctctg cggcgcagct
     7141 attcgctgtc gggcgagccg agcgagcact gttacaggat cagcgtcaag cgcgaaactc
     7201 atggcgcagc cggggcctat accgaggctc aagtcaaggt cggcgacgtg ctcgaggtga
     7261 gcgcgccacg c
//
LOCUS       DATLJT010000792         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3266567,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000792 DATLJT010000000
VERSION     DATLJT010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3266567"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="VM910_17965"
     CDS             <1..1986
                     /locus_tag="VM910_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI64445.1"
                     /translation="VVLTMPYVEMGQGTYTSIPMLIAEELDVSLKQVRLEHAPPNEKL
                     YANPLLGVQATGNSNAMRGAWKPLREAGATARIMLVEAAAKRWGVDPKSCKTEDGEVI
                     HVPTGRRLKYGELAAEAAKMDPPGHVALKSLQEFKLIGTPVKRLDAPAKVNGTAVYGI
                     DARPPGVKFATLAQSPTFGGRVKSVDDTAAKAVKGVRQIVRLDDAVAVVADHMGAAKK
                     GLEALKIEWDDGAHASLTTEAIARELEQATLGSGAVAQSVGDADKAMANAATKVEAIY
                     QVPFLAHATMEPMNCTVHFRKDECEIWIGSQAVARVQRMAAKAAGLPPEKVIVHNHLI
                     GGGFGRRLEADGAVRAVEIAKQVEGPVKVVWTREEDIQHDMYRPYWFDRISAGLDEQG
                     RPVAWNHRFAGSSVAARWLPPAFKNGLDADSTEGAIDLVYDLPNFHVEFVRVEPPGIP
                     TAFWRSVGPSHNVFVTESFIDEMAAAAKQDPVAYRRALLEKNPRAKAVLDLVAEKSGW
                     GQPLPQGRGRGVSLQNVFGSYQAHVAEVEVSKDGTVRVHRVVCAADCGTVVNPDTVQA
                     QIQSGVIFGTTAALYGEITLKNGRVEQGNFDTYQMIRINEAPAIEVHVVKSSEPPGGM
                     GETGTSAVVPAIANAVFAATGKRLRKMPIDAGALKPV"
     gene            2026..>2211
                     /locus_tag="VM910_17970"
     CDS             2026..>2211
                     /locus_tag="VM910_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HVI64446.1"
                     /translation="MIRKAKAVWRGTGRAGSGNLTTDSGVLAETPYSFKTRFESEKGT
                     NPEELIAAAHAGCFTMAL"
BASE COUNT          447 a          651 c          747 g          366 t
ORIGIN      
        1 gtcgtgctga cgatgcccta tgtcgagatg gggcagggca cctatacgtc cattccgatg
       61 ctgattgccg aagagctcga tgtgagcttg aagcaggtgc ggctcgagca tgcgccgcca
      121 aacgagaagc tttacgccaa cccattgctg ggcgtgcagg cgaccggcaa ttcgaatgca
      181 atgcgcgggg catggaagcc gctgcgcgag gccggcgcaa cggcaaggat catgctggtc
      241 gaggctgcgg ccaagcgctg gggcgtcgat ccgaaatcct gcaaaacgga ggatggcgag
      301 gtgatccatg tgccgaccgg ccggcgcctg aaatatggcg agctggccgc ggaggccgca
      361 aagatggacc ctcccggaca tgttgcgctg aaatcactac aggagttcaa gctgatcggc
      421 acgccggtca aacggctgga tgcaccggcc aaggtcaacg ggaccgcggt ctatggcatc
      481 gatgcgaggc cgcccggcgt aaaattcgca acgcttgcgc agtcgcccac cttcggcggg
      541 cgggtgaaga gcgtcgacga tacggctgcc aaggccgtca agggcgtgcg ccagatcgtg
      601 cggctcgacg acgcggtcgc ggtcgtggcc gaccacatgg gcgctgcgaa aaagggcctc
      661 gaggcgctga agatcgagtg ggacgacggc gcgcatgcaa gcctcaccac cgaagcgatc
      721 gcgcgcgaac tggagcaggc gacgcttggt tcgggcgcgg tggcgcagag cgtcggtgac
      781 gccgacaagg ccatggcaaa tgctgctacc aaggtggagg cgatttatca ggttccgttc
      841 ctggcgcacg ccacgatgga gccgatgaac tgcacggtgc actttcgcaa ggacgaatgc
      901 gaaatctgga ttggcagtca ggcggtggcg cgcgttcaga ggatggccgc gaaggccgcg
      961 ggcctgccgc cagagaaggt catcgttcac aatcatctga tcggcggcgg ttttggccgg
     1021 cggctggagg ctgatggtgc ggttcgcgcc gtcgaaatcg caaagcaggt cgaaggtccg
     1081 gtcaaggtcg tgtggacgcg cgaggaagac attcagcacg acatgtaccg gccttactgg
     1141 ttcgaccgca tttccgcggg gctcgacgag cagggcaggc cggtcgcctg gaaccatcgt
     1201 ttcgcaggct cgtcggtcgc cgcgcggtgg ttgccgccgg cgttcaaaaa tggcctcgac
     1261 gcggactcga cggaaggcgc catcgatttg gtttacgatc tcccgaactt ccatgtcgaa
     1321 tttgtgcgcg tcgaacctcc gggcattccg accgcgttct ggcgcagcgt cggtccctcg
     1381 cacaatgtct tcgtcaccga aagctttatc gacgagatgg cagcggcggc gaaacaggat
     1441 ccggtcgcct atcgacgggc tctcctcgag aaaaacccgc gcgcgaaagc ggtgctcgat
     1501 ctcgttgccg aaaaatcggg ttggggccag ccattgcccc aagggcgcgg gcgcggtgtc
     1561 tcgctgcaaa atgtcttcgg cagctatcag gcgcatgtag ccgaggtcga ggtttcgaaa
     1621 gacggcacgg tgcgcgttca ccgcgtggtc tgcgcggccg attgcggcac cgtcgtcaat
     1681 cccgacacgg tgcaagcgca gatccagagc ggcgtcatct tcggtaccac cgcggcgctc
     1741 tacggagaga tcacgctgaa gaacggccgg gtcgaacagg gcaatttcga cacctatcag
     1801 atgatccgca tcaacgaggc gccggccatc gaggttcatg tcgtgaaaag ttcggagccg
     1861 cccggcggca tgggcgagac cgggacatcc gcagtcgttc ccgccattgc caacgcagtc
     1921 tttgccgcaa cgggaaaacg cctgcgcaag atgccgatcg atgccggcgc gctgaagccg
     1981 gtgtgagcga agatacctcg accatgaaac gcaaaggaga ctcgcatgat tcgaaaagcg
     2041 aaagcggttt ggcgcggcac tggccgcgcc ggcagcggca atctcacgac cgactccggc
     2101 gttctcgcgg agacgcccta ttcgttcaaa acacgcttcg agagtgagaa aggcaccaac
     2161 ccggaagagt tgatcgcggc cgcacatgcc ggttgcttca ccatggcgct g
//
LOCUS       DATLJT010000793         1782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1888422,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000793 DATLJT010000000
VERSION     DATLJT010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888422"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VM910_17975"
     CDS             <1..549
                     /locus_tag="VM910_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI64447.1"
                     /translation="TEFFKDGAYRYEGESKVRSRQEQVNYLAELVSRYPIVSIEDGMA
                     EDDVEGWKMLTDLIGDKCQLVGDDVFVTNVTRLAHGIKNGIANSILVKVNQIGTLTEA
                     LAAVEMAHKAGYTAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRTAKYNQLL
                     RIEEELGGQARYAGRGALKALR"
     gene            627..944
                     /locus_tag="VM910_17980"
     CDS             627..944
                     /locus_tag="VM910_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVI64448.1"
                     /translation="MVTRKRFRTVLNALALYTIAALVIGYFGVNAYSGNRGLRAKQDL
                     DQQIAELSAELDALKAERANWERRVSLLKPQSIDPDMLDERARVLLNYADPRELTLRL
                     KQP"
     gene            1114..>1782
                     /locus_tag="VM910_17985"
     CDS             1114..>1782
                     /locus_tag="VM910_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI64449.1"
                     /translation="MAVASNRPAAQPAAVRRASVEPAAELPDFNQEQELHAYRYMLLI
                     RRFEEKAGQLYGMGLIGGFCHLYIGQEAVVVGMQMAIKGGDEVITGYRDHGHMLACGM
                     DPKGVMAELTGRRGGYSKGKGGSMHMFSVEKGFFGGHGIVGAQVPLGTGLAFANKYRG
                     NDNVSVTYLGDGAANQGQVYESFNMAKLWKLPVIYIIENNQYGMGTSVARASATTNLS
                     QRGRS"
BASE COUNT          357 a          575 c          544 g          306 t
ORIGIN      
        1 accgagtttt tcaaggatgg tgcctatcgc tacgagggcg agagcaaggt tcgctcgcga
       61 caagagcagg tgaactacct ggccgagctc gtctcgcgct atccaatcgt ttcgatcgag
      121 gatggaatgg ccgaggacga cgtggaaggt tggaagatgc tgaccgacct gatcggcgac
      181 aaatgccagc tcgtcggcga cgatgtattc gtgaccaacg tcacgcggct cgcccacggc
      241 atcaagaacg gcatcgccaa ctccattctc gtcaaagtca accagatcgg cacgctcacc
      301 gaggcgctcg ctgcagtcga gatggcgcac aaggccggct acaccgcggt catgtcgcac
      361 cgttccggcg agaccgagga tgcgaccatc gccgatcttg cggtcgcgac caactgcggg
      421 cagatcaaaa ccgggtcgct cgcgcgctcg gaccgcacgg cgaaatacaa tcagctcttg
      481 cgcatcgagg aggaattagg cggccaggcc agatatgccg ggcgaggcgc gttgaaggcg
      541 ttgcgctgat atcacgcggg tcgactgagg tccgcggcat ccagcgcatt aaagccgtct
      601 taacgctgcg ccggcaaggt tgcggcatgg tgacccgcaa gcggttccgc accgtcctca
      661 atgcgctcgc gctttacacc atcgccgcgc tggtaatcgg ctatttcggc gtcaacgcct
      721 attcgggcaa ccgcgggctt cgcgcgaagc aggacctcga ccagcagatc gcggaactca
      781 gtgccgagct tgacgcgctc aaggccgaac gcgccaattg ggaacgccgc gtttcgctgc
      841 tcaagcccca aagcatcgac cctgacatgc tcgacgagcg cgcccgggta ctgctcaact
      901 acgccgatcc gcgcgaactc accctgcgcc tcaagcaacc ctgaccacgc tggcccgcta
      961 ccgcccgcga tttgcgaaat cggttttttc cacctatttc ccgccgcaaa gagccgcgcc
     1021 cgcgcttatc cccatccgct tccgccttgc ggcgatttcg gctattgggg aagcggccgc
     1081 ctcgccgacg aagagcccac cccgaggaga aaaatggccg ttgccagcaa tcgacccgcg
     1141 gctcaacccg ccgccgtgcg ccgggcatcc gtcgaacctg cggcggagtt gcctgatttc
     1201 aaccaggagc aagagcttca cgcctatcgc tacatgctgc tcatccgccg gttcgaggag
     1261 aaggccggac aactgtacgg catggggctg atcggcggct tctgccacct ctatatcggt
     1321 caggaggccg tcgtcgtcgg catgcaaatg gcgatcaagg ggggcgacga ggtcatcacc
     1381 ggataccgcg accacggtca tatgctggcc tgcggcatgg accccaaggg cgtgatggcg
     1441 gagttgaccg gccggcgcgg cggctattcc aagggcaagg gcggctcgat gcacatgttt
     1501 tcggtcgaga aagggttttt cggcggacac ggcatcgtcg gcgcccaggt cccgctcgga
     1561 actggcctcg ccttcgccaa taaataccgg ggcaatgaca atgtttcggt cacttacttg
     1621 ggcgacggcg ccgccaacca ggggcaggtc tacgaaagct tcaacatggc gaagctgtgg
     1681 aagctgccgg tgatctacat catcgagaac aatcagtacg ggatgggaac ctccgttgcc
     1741 cgcgcatcgg cgaccaccaa cctgtcccag cgcgggcgtt cc
//
LOCUS       DATLJT010000794         4803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_737091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000794 DATLJT010000000
VERSION     DATLJT010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1153)
                     /locus_tag="VM910_17990"
     CDS             complement(173..1153)
                     /locus_tag="VM910_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64450.1"
                     /translation="MKRRNFITLLGSAAAAWPLAARAQQPAMLVIGFLGSESPDLFAN
                     RLRAFREGLSENGYIEGRNVTFEFRWAEGQYDRLPEMAADLVRRHVSVIAANGPPAAL
                     AAKAATSTLPIVFSGGIDPVKLGLVVSLNRPGGNVTGVSQFSTVLEGKRLELLHKLVP
                     NAAVIAMLVNPTFPGTDSITNDIQIAARALGLKLNVLNASSEHDFDTAIARIVQLRAG
                     ALIVASDPFFFSRRDQLVTLVAHHAVPAIYQFREFAAAGGLMSYGTNLTDAYRQVGIY
                     VGRIFKGEKPADLPVVQPTKFDLVINLTTAKALGLTVPPTLLALADEVIE"
     gene            complement(1150..1971)
                     /locus_tag="VM910_17995"
     CDS             complement(1150..1971)
                     /locus_tag="VM910_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI64451.1"
                     /translation="MAAVVAEYLDSRLFFGSKSPGTQRMRRGILERFRAAYGERPFAL
                     LPPEWIEALLDAKPPHAARSWLVTLRSLCQFALKRGYVRADPTANIKQRAIQGDGFHT
                     WDEDEIAQFEAHHPVGTKPRLALALLLYTAQRRSDVVKMGRQHIRDGWLHVKQEKTDK
                     PLAIPVHPELRAVLDATASEHLTFLVTATGKPYGPNHFSETFREWCDAAGLPKRCTAH
                     GLRKAACRRFAEAECSGPEIMSISGHATLKELVRYTKAADQAKMARNAMAKVVIR"
     gene            2130..2903
                     /locus_tag="VM910_18000"
     CDS             2130..2903
                     /locus_tag="VM910_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017268922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64452.1"
                     /translation="MTAELYRASPIKRTRATKAAVEARRETLLDIIAVGRPMTVRQVF
                     YQATVHGLVEKAESGYGKVQTDLTVMRRAGELPYDWLADNTRWQRKPRTFSSVEEALR
                     DTAAFYRKSLWTDADCYVEIWLEKDALSGVIYPVTSMYDVPLMVARGYASLSFLYSAA
                     EYINDLDVPTYIYHFGDFDPSGVNAGEKIEETLRELAPGADIVFERIAVTEAQIADWD
                     LPTRPTKASDTRAKAFGSTLSAAWSDPAPEHGPTGGLAP"
     gene            3214..4794
                     /locus_tag="VM910_18005"
     CDS             3214..4794
                     /locus_tag="VM910_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVI64453.1"
                     /translation="MSFVGAVLALVVVSLFSMVASAQTTGCTASSDVACTEQGAVRGA
                     VQGATMAFKGIPYAAPPVGSLRWRPPTPPARWDGVREGSRFGAICPQIVGGEVKGDED
                     CLYINVWRPREKPDRPLPVMVWVHGGGNHRLSGEGSEQFGGVAYNGEQLVPQGVVFVS
                     YNLRLGVLGFLSHAALSAERPEKISGNYGSLDQIAMLRWINRNIAAFGGDASRVFLFG
                     TSAGGGNICALMTSPLTRDLIHRVAMESSVPAGCEIKTLAEDENDTGQRVARAAGCDT
                     APDVAACLRGKSVTEIVKAVPGTFSVLARIYGPNMDGHVFPEQPIKIIAAQRHPPMPI
                     IIGNTSRETWGWSSTVTDEASYAATIDKAFGATARDPILKLYPASAYSSPRAAFVQAT
                     TDAQFTCVSRRVARTFSKAQREPVFRFIFDHTLENDPQLKALGANHTIEHPFFFAWQG
                     RYRPTETDLAVQRRIVGYWVRLAKSGNPNGGGDPEWPAESRNDAYLEIGAVTAAKSGP
                     ASAHCDFWDEVPMLWPHL"
BASE COUNT          881 a         1494 c         1530 g          898 t
ORIGIN      
        1 taaccgagtt cacgcatgcc gagctggaat ccggcgtaca agcgggaata ggtgtcgttt
       61 gacgctccgg agagcaagcc gatgcgccag acctttcccg gctgctgcgc gcgcgccgcg
      121 agcggccacg cggccgccgc gccgccgagc agcgtgatga actcgcgcct tttcactcga
      181 tcacctcgtc agcgagcgcg agcaatgtcg gcggcacggt caggccgagc gccttggcgg
      241 tggtgaggtt gatgacgaga tcgaacttgg ttggttgcac cactggaaga tcggcgggct
      301 tctcaccctt gaaaatccta ccgacataga tgcctacctg gcgatatgca tcggtaagat
      361 tggttccgta gctcatcagg ccaccggccg cggcgaactc tcgaaactga taaattgcgg
      421 gcactgcatg gtgagctacc agggtgacaa gctgatcgcg tcggctgaag aagaatgggt
      481 cagacgctac gatgagcgcc ccagctcgta gttgaacgat cctcgcaatg gcggtatcga
      541 agtcatgttc gctgctggca ttcaggacat tcagttttag cccaagcgcg cgcgcggcga
      601 tctgtatgtc attggtaatt gaatccgtgc ccggaaaagt cgggttcacg agcatagcta
      661 tcacggcggc attgggaacc aacttatgta gcaactccag gcgctttccc tctagcacag
      721 tgctgaactg gctgacgcct gtgacgttgc cgcctggtcg gttaaggctg acaacgaggc
      781 ccagcttgac cgggtcaatg ccaccggaga agacgattgg aagcgtcgag gttgctgcct
      841 tggctgcgag cgctgcaggc gggccgttcg cagcaatcac ggacacgtgg cgccgaacca
      901 gatcggctgc catttccggc agccggtcgt attggccctc cgcccagcga aattcgaacg
      961 tcacgttccg gccctcgata tagccgtttt cgctcagccc ttcgcgaaac gcgcgcagac
     1021 ggtttgcgaa taggtcaggc gattcgctgc cgaggaaccc gatcaccagc atcgcgggct
     1081 gctgcgcccg cgccgcgagc ggccacgccg ccgccgcgct gccgagaagc gtgatgaaat
     1141 tgcgacgctt catcggatga ccaccttcgc catcgcattg cgggccatct tcgcttggtc
     1201 cgccgccttg gtgtagcgga caagctcttt gagcgtggcg tgtccactga tcgacatgat
     1261 ctcggggccg ctgcattcgg cctcggcgaa tcgacggcag gcggccttgc gcagcccgtg
     1321 cgcggtgcag cgctttggca gtccggccgc gtcgcaccac tcgcggaaag tttcgctgaa
     1381 atgattcggg ccgtatggct tgccggtcgc ggtgactaag aacgtcaagt gctcgctcgc
     1441 cgtggcgtcg agcaccgcgc gcaattcggg atgcaccggg atcgcgagcg gcttgtcggt
     1501 tttctcctgc ttcacatgca gccagccgtc gcggatatgc tgccgcccca tcttgacgac
     1561 atcgcttcgg cgttgcgcgg tgtagagcaa gagcgcgagc gcaaggcgcg gcttcgtgcc
     1621 gaccggatga tgcgcctcga attgcgcgat ctcgtcttcg tcccacgtgt ggaatccgtc
     1681 gccctgaatg gcgcgctgct tgatgttggc ggtcgggtcc gcgcgcacat agccgcgttt
     1741 gagcgcgaac tgacaaagcg agcgcaaggt gacgagccag ctacgcgcgg cgtgcggcgg
     1801 cttggcgtca agtagcgcct cgatccattc cggcggtagc agcgcgaacg gcctctcgcc
     1861 gtaggcagcg cggaagcgct caaggattcc ccggcgcatg cgttgcgtgc ccggactttt
     1921 cgagccgaag aatagtcgcg agtcgagata ttcggcgacg accgctgcca cgctgccggg
     1981 cttgacgcga cccgcgccgt tgtatccaaa tcatgtgatt ccaagggctt gcgctgtctg
     2041 ggtgcgctta aagcgcacat tggcagcatt agcacatttc gcccggtgtc tgggatgccg
     2101 ctcgttcccg ccgaccggga ggcggcgcga tgaccgccga gctttatcgg gctagtccca
     2161 taaaacgcac ccgcgccacc aaggcagcgg tggaggcccg ccgtgagacg ctgctcgaca
     2221 tcatcgcggt gggcaggccg atgacggtcc ggcaggtgtt ctaccaggcc acggtccatg
     2281 gtctcgtcga gaaggccgag agcggttacg gcaaggtgca gactgatctc acggtgatgc
     2341 gccgggcggg cgagcttccc tatgactggc tcgccgacaa cacccgctgg cagcgcaagc
     2401 cgcgcacttt cagcagtgtc gaggaagcac tcagagatac cgcagcgttc taccgcaaga
     2461 gcctgtggac cgacgccgac tgctacgtcg aaatctggct ggagaaggac gcgctctcgg
     2521 gcgtgattta tccagtgacg tctatgtacg acgtgccgct gatggtggcg cgcggctacg
     2581 ccagcctgtc gttcctgtac agcgcggccg agtacatcaa cgacctggac gttccgacct
     2641 acatctacca tttcggcgac ttcgatccgt ccggcgtcaa tgccggggag aagatcgagg
     2701 agacgctgcg cgagctggca ccgggtgcgg acatcgtgtt cgaacgcata gcggtcacag
     2761 aggcgcagat cgccgactgg gatttgccca cccgcccgac caaagcctcg gacacccgcg
     2821 ccaaagcgtt tggcagcacc ctttccgccg catggagtga cccggccccg gagcatggac
     2881 cgacgggggg cctcgcgcct tagaggcgct cattcgcgat tgccgaacaa tcgcgattta
     2941 ccttctaggg cgcactcata aatccgcttc tgtaagcgcg gctcagtcaa tgtccgcttc
     3001 gctcagaaag cgaccgaagt gctgcgctgc cgcgaataga cgcgatgtgc cacaagctga
     3061 cgtcacgccc gggagatgag ccgaacggcg gtcgaagcga cgggccggat tccgacagtg
     3121 gcattagaat tcgggcgacg tctttcgaca ccttgcaacg cggggtactc ggggaccact
     3181 atggtgtcaa aagctcgttt aggggggaac tccatgtcgt tcgtcggagc tgtactcgcc
     3241 ctcgtcgtcg tcagcctttt ctcgatggtc gcatcggcgc agaccacagg ctgcaccgcc
     3301 agctccgatg tcgcctgcac tgagcagggc gccgttcgcg gcgcggtcca aggcgccacg
     3361 atggccttca aaggaattcc ctatgcggcg ccgccggtcg gctcgttgcg gtggaggccg
     3421 cccacgccac ccgcgcgctg ggatggtgtg cgcgagggca gccgcttcgg agccatttgc
     3481 ccgcaaatcg ttggtggaga agtgaagggc gacgaggatt gtctgtacat caatgtctgg
     3541 cggccgcgcg agaagccaga tcgtccgctg ccggtgatgg tgtgggttca tggcggtggg
     3601 aaccacagac tatcgggcga aggctccgag cagttcggtg gcgtcgccta caacggcgag
     3661 caactggttc cgcagggcgt cgtcttcgtc tcctataacc tgcgcctcgg cgtattgggt
     3721 ttcctgtcgc acgccgcgct cagtgctgaa cgccctgaga agatttcagg gaattacggc
     3781 agtctcgatc agattgcgat gctgcgatgg atcaatcgga acatagcggc tttcgggggc
     3841 gatgcgtccc gtgtgttcct gttcggtacc tcagccggcg gcggaaacat ctgtgcgctg
     3901 atgacgtcgc cactgacgcg cgaccttatt caccgcgttg ccatggaaag cagcgttccg
     3961 gccggctgcg agatcaagac gctcgccgag gatgaaaacg atactggcca gcgggtggcc
     4021 agggctgccg gctgcgacac cgcgcctgac gtcgcggcct gcctgcgcgg caagagcgtg
     4081 acggagatcg tcaaggcggt tcccggcacc ttcagcgtgc ttgcgcgcat ctacgggccc
     4141 aacatggacg gccatgtctt tccggagcag ccgatcaaga tcattgcggc acagcgccat
     4201 ccgccgatgc caatcatcat cggcaacacg tcgcgcgaga cctggggctg gtcgagcacg
     4261 gtcaccgacg aagcaagcta cgccgcgaca atcgacaaag ctttcggcgc caccgcgcgc
     4321 gaccctattc tcaagcttta cccagcgagc gcgtattcgt ccccgcgcgc cgcgtttgtg
     4381 caagccacga cggacgccca attcacgtgc gtcagccgcc gcgtcgcccg caccttctcc
     4441 aaggcgcaga gagagccggt tttccgtttc atctttgatc acacgctcga aaacgatccg
     4501 caactgaagg cgcttggcgc aaatcacacc attgagcacc cgttcttctt cgcgtggcaa
     4561 ggccggtacc gcccgacgga gactgatctc gccgttcagc ggcggatcgt cggctattgg
     4621 gtgcgcctcg caaaatccgg caaccctaac ggcggtggcg acccggaatg gccagccgag
     4681 tccaggaacg atgcctacct ggaaatcgga gctgtgactg ccgcaaagtc aggtccggcg
     4741 agtgcgcatt gcgacttctg ggatgaggtg ccgatgctct ggccgcacct ctagtgacat
     4801 att
//
LOCUS       DATLJT010000795         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_968366,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000795 DATLJT010000000
VERSION     DATLJT010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968366"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VM910_18010"
     CDS             complement(<1..717)
                     /locus_tag="VM910_18010"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI64454.1"
                     /translation="MKGVVFLGDRKLELRRFPDPTPGPRDVILEIKASGMCGSDLHTY
                     RAPANPGGVVTGGIKRAAGVIAGHEPCGVVAAVGAAVTDKEARIGQRVMDHHYDGCGT
                     CKHCRAGWTQMCLEGPVVYGSGGHGGHAKYMKVPVSTLVPLPDSLSFVTGAAISCGTG
                     TAWGALRRIQLQGGETIAIFGQGPVGLSATQLAVEMGARVIAIDIAPERRKLARQFGA
                     AEVIDPTAHDMVAAIAELTHG"
     gene            995..>2208
                     /locus_tag="VM910_18015"
     CDS             995..>2208
                     /locus_tag="VM910_18015"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI64455.1"
                     /translation="MAPSDYLQRFWLCFAIFGTVVILLAAGIYVVETLPPRRIVMATG
                     AQGGAYHELGIRYRQLLTEAGIELQLLPTTGAIENLMRLRDPRSGVDVGFIQGGTTTQ
                     KESPGVESLGTLFYEPLWFFTRISGSRTEGMSGRRFSIGPEGSGTRDLVLQLFEKNKI
                     AAELLGLSPDVAAEKLIAGDIDGMFMVATWDSPVVQQLIMAQGVELRSFPRADAYIQL
                     FPFLNRVVLHEGVADLATNRPPSDVVLVAPKASLAVRTDLHPAIQYLLLGAAVEIHSG
                     PGLFRKAGQFPAAESIDIPLSDEAQRYYKSGQPFLHNYLPFWIAELVARVFIVFVPVA
                     LLLYPLFKLLPRTYDWIMRSKITPLYNELRSIEREMDAQGSGHAADAIVARLDQLDQR
                     ATRLRLPTSYASA"
BASE COUNT          394 a          666 c          702 g          446 t
ORIGIN      
        1 gccgtgcgtc agctcggcaa tcgccgcgac catgtcgtgc gccgtgggat cgatcacctc
       61 ggcggcgccg aactgccggg cgagcttgcg tcgctcgggt gcgatgtcga tggcgatgac
      121 gcgcgcgccc atctcgacgg cgagctgcgt cgcggagagg cccaccgggc cctggccgaa
      181 gatggcgatc gtttcgccgc cctgcagctg gatccgccgc agcgcgcccc aggccgtgcc
      241 ggtgccgcag gagatggcgg ccccggtgac gaaggagagc gagtcgggca gcggcaccag
      301 cgtcgacacc ggcaccttca tgtacttggc gtggccgccg tgaccgccgg agccgtagac
      361 caccggtccc tcgaggcaca tctgcgtcca gcccgcgcgg cagtgcttgc aggtgccgca
      421 gccgtcgtaa tggtggtcca tcacgcgttg gccgatgcgg gcttccttgt cggtgacggc
      481 ggcgcccact gccgcgacca cgccgcaggg ctcgtgaccg gcgatcacgc cggccgcccg
      541 cttgatgcca ccggtgacga ctccacccgg attggccggg gcgcggtagg tgtgcaggtc
      601 gctgccgcac atgcccgagg ccttgatctc gaggatcacg tcgcgcgggc cgggtgtcgg
      661 atcggggaac cgtctcagct ccagcttgcg atctccgaga aacacgacgc ctttcatggt
      721 tctcctccgg tgcgcgctgt tcggcgtgcc cgtatgacga gggcgtgtac tcataaacgc
      781 gggctttggc cgaatgtcgg ctgttggggc gaaccggaca cgccttgatg gcgggactga
      841 cgtgaatgac ccgaagcgga catcgacgcg ctaccaggat ttgaccgcat ccaaggctga
      901 cgctccacca ccaagcgggc tgtagacaaa tcggcctggc accgcggtcg ctggctggta
      961 tgctcaaagc ggcctcgcgg agggagcaaa cttaatggcc cctagcgact atttgcaaag
     1021 attctggctc tgcttcgcga tctttgggac cgtcgtgata ttgctcgctg cgggcattta
     1081 cgttgttgag acgttgccgc cgcgcaggat agtgatggcc accggcgccc aaggcggtgc
     1141 ttaccacgaa ctgggtattc gctaccgtca gctcctgacg gaggcaggca tcgagctgca
     1201 gctgctgcca acaaccggag ccatcgagaa tctgatgcgg ctgcgggacc ctcggtccgg
     1261 ggtggatgtt ggattcatcc agggcgggac taccactcag aaggaatcgc ccggggtgga
     1321 gtcgctgggc acgttatttt acgagccgct gtggttcttc acccgtatca gtggcagtcg
     1381 tacagaaggg atgagcggcc gacgattttc gattggtccc gagggaagcg ggacgcgaga
     1441 cctggtgctc caactgtttg agaagaacaa aatcgccgct gaattattgg ggttgtcgcc
     1501 agatgtagcg gccgaaaagc taatcgcagg cgatatcgac ggcatgttca tggttgcgac
     1561 atgggattcc ccggtcgtcc agcaactcat catggctcaa ggtgtcgagc ttcggagttt
     1621 tcctcgcgcg gatgcttaca tccaactttt cccttttctg aatagggtag tgctccatga
     1681 gggcgtagcc gatcttgcga cgaacagacc gccctcggat gtcgtgctgg ttgccccgaa
     1741 ggctagtcta gccgtgcgca cggacctcca tcccgccatt cagtatctgt tgctcggcgc
     1801 ggccgttgag attcattctg ggcccgggct cttccggaaa gccggacaat tccctgcggc
     1861 tgagtcgatc gatattccgt taagcgatga agcacagcgc tactacaaaa gcgggcaacc
     1921 gttcctgcac aactatcttc cgttttggat tgcagagcta gtcgcacgag tgttcatcgt
     1981 ttttgtccca gtagcgctct tgctgtatcc cttattcaag ctcttgccgc gaacctatga
     2041 ctggatcatg cggtcaaaga taacgcccct ctacaatgag ctcagatcga ttgagcgtga
     2101 gatggatgct caaggctcgg gacatgccgc cgacgccatt gttgcgagac tcgatcaact
     2161 ggatcaacgt gcgactcggc tacggttgcc aacttcttac gcaagtgc
//
LOCUS       DATLJT010000796         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3497473,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000796 DATLJT010000000
VERSION     DATLJT010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3497473"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VM910_18020"
     CDS             <1..198
                     /locus_tag="VM910_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64456.1"
                     /translation="RRLFLGLLPPPAFCERRHRGLARRLEPCVGVPAKRREAAVEASA
                     ASGIGGSMGQRIPGYGRPAAV"
     gene            380..859
                     /locus_tag="VM910_18025"
     CDS             380..859
                     /locus_tag="VM910_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64457.1"
                     /translation="MFIGACLRHTCFPQGQASPLASHAVTKYQNINSVPGLEGRFNSF
                     FGFTALKVNLASFGSACLEGDALVSIRPFLNGQTFDPQTARLVGIAFETARAAINRPG
                     DLSDEMIARAIIELAKAGERNVEVLCEAALNLPAGAVSAPNPPTLAASPPSKPGSLS"
     gene            complement(1958..2587)
                     /locus_tag="VM910_18030"
     CDS             complement(1958..2587)
                     /locus_tag="VM910_18030"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HVI64458.1"
                     /translation="MAITASTTACSRSCSWVGAFGNRTPESFARGRSYFDDSAVSDLV
                     RRGDRLTAEVEGSELAPYRVSIRLHDGGVTEARCSCPYDLGGYCKHIVAVLLKFADEG
                     TRAIERKPLAKLLRELDQSRLIELLEKRAESDSELAAWMEAELATTVEPSSLHNKEVG
                     RRRTPVDGYLASSGGDRAKFGVNCLSRRVVVENGTGEADRRLPRTGYRP"
     gene            complement(3189..>3303)
                     /locus_tag="VM910_18035"
     CDS             complement(3189..>3303)
                     /locus_tag="VM910_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVI64459.1"
                     /translation="RSPVIVPIPGTSSVAHLDENVAAARILLSTEDLHDLS"
BASE COUNT          607 a         1040 c          997 g          659 t
ORIGIN      
        1 cgtcggcttt tccttggtct actcccgccg ccagcttttt gcgaacgccg tcatcgcggc
       61 cttgcgcgtc ggctcgagcc gtgcgtgggt gtgcccgcta agcggcggga ggcggcggtg
      121 gaggcttcgg cggcctcggg gatcggcggc agcatggggc aacgcattcc cggttatggc
      181 cgccctgcgg cggtgtgagc catcctacac cctcggggcg tcctccccgt ttattactgc
      241 cggcgcccct ggccttgtga agcgatgtac ttgagttggc tataatctca cccgcccgac
      301 gtgcaaaggc ctcgtcatcc acgggtgcag gctgcggcat atcgcgcccc agccagagcg
      361 gcggctcttc ctggttctgt tgtttatcgg ggcgtgtctt cggcatacgt gcttcccgca
      421 agggcaggcg tcacccctcg cgtcgcatgc tgttacgaaa taccaaaaca tcaattccgt
      481 tcccggcctg gaaggccgat ttaattcgtt ttttggtttc acagcgctta aagtgaattt
      541 ggcgagcttt ggttcagctt gccttgaggg ggacgccctc gtgtccatta gacctttctt
      601 aaacggccag accttcgatc ctcagaccgc ccgtctcgtg ggcatcgcat tcgaaacggc
      661 ccgagcggcc attaaccgcc caggcgacct cagcgacgaa atgatcgcca gagcgatcat
      721 cgagcttgcg aaagccggtg agcgcaatgt cgaagttttg tgtgaggccg cactgaacct
      781 gcctgcggga gcagtcagcg cccccaatcc tcctacgctt gccgcttcac cgccgagcaa
      841 gccgggttct ttatcttgag ccaatcaggc gagcgccccg agcgatacat cgagtccttg
      901 cgcttcgaca cgatgccttc gagtcccatc ttgcaggcgt ggacaaaaat ggtggtggcg
      961 aatgagatcg aacgacgcca cgccgttgtc ggcaagctac gcgccctgcg gcctttcggc
     1021 gtcctctgat gttttgtccg ctgagcgccg cgcgcgcgac aaattcccgg cccctatcag
     1081 tctatcgcta aggccctcat tgccgggctt ctcaagttcc cgagccagtt ggggatgcac
     1141 gaagacctta ccattttcgg cgatgtacac tggtgcctcg ccgatgcggg tatgaacgtc
     1201 attcataagt gccaacaacc gctgagaatc cgtctcggcg cgcccctcaa gcaaatcgct
     1261 caaacctctc atgctctagg ccgtgtgcgc tctcacggtc tcggccgtag gcgctagcag
     1321 ctgcggctgg gttgccttgt gcgagcggcg acgagctggt ttcgctaggc tttcgggttg
     1381 aagcgcttcg ccgggacatc aaaggatagg tcgcaagcgg gcccggcctc tcgaataccg
     1441 cagccagcta gccccaaatt attcgtggag caggcgcttg gcgatgcaag cgcaggggct
     1501 caaggcttga acgtgcggat agggctgagg gcagcgccat cttgatgcgg gtcttgagct
     1561 cttcgacgcg tacagcgatc caggaacgcg gggcggatgg tctccaaggc cgctgagacc
     1621 catccgccgt cattaccagg catagcgcgt cctcaaaggc gttgacgcca ctccatagac
     1681 cgcgaagacc ggggtcacgc cctgaaggcg ggctatggac gcgcgtaaac gcgcttatgg
     1741 cgtccaattc cgctcggctc atttcacatt attgccccac aacttccatg cgttggtcga
     1801 aactggcggt cagaacatgc gtcgtggtga atagcgtggg ggagacgggg atgtatctct
     1861 tggccgtttt ccgagccatt acagccgtct tgacgcaccg ccgctatcga ccggagctgc
     1921 actacatgcg cggcccggga ccgaagtggt tcgagcgtca cggccgatag cctgttcgcg
     1981 gaagccgccg gtcggcttcg cccgtgccgt tctcaacgac gacacggcgg gataggcagt
     2041 taactccaaa tttagctctg tcgcctccgc tcgatgcgag ataaccgtcg acgggggtcc
     2101 gtcgccggcc gacttccttg ttgtgtagtg acgacggctc gaccgtcgtc gcgagctcgg
     2161 cctccatcca cgcggcaagt tccgagtcac tctcggcacg cttctccagc agctcgatca
     2221 gccgggactg atccaactcg cggagcagct tggcgagcgg cttgcgttcg atggcgcgcg
     2281 tcccttcgtc cgcaaatttc agcaggaccg cgacaatatg cttgcaatat ccacccaaat
     2341 cataggggca gctgcaccgg gcttcggtga ccccgccatc gtgcagccgg atagaaaccc
     2401 ggtagggcgc aagctcgctg ccctccactt ccgccgtcaa tcgatcgccg cgccggacga
     2461 gatcggagac cgcactgtcg tcaaagtagc tccggccgcg cgcgaaggac tcgggcgtac
     2521 ggtttccaaa cgcgccgacc caagagcagg agcgcgagca cgcagtcgtc gatgcggtga
     2581 ttgccatctc cgcacttctg gatcatgccg ttagagaacc gccagcgctc gagttgtgcg
     2641 cggtcgcggg ttctctccca cgataacgtg agtcgcggtg ccggcgatag tgacagcggg
     2701 tgcgcagctg atggcgcggg cgttggtcac caagctgcgt atccctgaca ccgcagctca
     2761 aacgcgaaag tctccagtgg cccgttcagg ccgagccgag gcgagttgtc gcggcatctc
     2821 aaatacatgg ctcgcgcacg gatattcgag ttcgagtctc accaccccag ccacgcagtc
     2881 cgctctctgt ggctcatatg cggcacctca aaaaatgcac gacattctat acggttagcg
     2941 cgactaaagc gcgcgcggcc gcgagcaggg gcgtggcgct cgcccgaacg atcagctccg
     3001 ttccgagact cgcccttcgc caaaaagcgg ttcgtcgatc acaccgtctg cgacacgaca
     3061 attgggtgat cggctcccga tgtgggcgaa acagctttag ttccggaacg actagcgtcc
     3121 tcaaagccgt gtttgccaga cgagatgaac caatggcttc ggcacccgcg ttgccagaag
     3181 caaccgtttc aggacagatc gtgcagatct tcggtcgaaa ggagaatccg cgcggcggcg
     3241 acgttctcgt caaggtgcgc caccgatgat gtcccgggaa tgggaacaat gacgggcgag
     3301 cgg
//
LOCUS       DATLJT010000797         1700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4416094,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000797 DATLJT010000000
VERSION     DATLJT010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4416094"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VM910_18040"
     CDS             <1..531
                     /locus_tag="VM910_18040"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64460.1"
                     /translation="ARLAAFLQELQQLGWVEGRGVRIDVRWGAGDAERIRRYATELVG
                     LTPDILMGSTTPPVAALQQATATVPIVFAQVGDPVGAGFVDSLARPGGTTTGFSVFEF
                     SFGGKWLELLKEIAPTVTRVAVIRDPAIASGMGQWGAIQSAAPPFASSCVQSTCAMPG
                     RSNAPLRPSRASLIAG"
     gene            465..851
                     /locus_tag="VM910_18045"
     CDS             465..851
                     /locus_tag="VM910_18045"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64461.1"
                     /translation="MRDAGEIERAVTSFTRLPDSGLIVTGSPLAYFHRELIIALAARY
                     RLPAVYSDRVYVADGGLISYGPDRVDQFRLAAGYVDRILKGEKPADLPVQAPTKYELV
                     INLKTAKALGLDVPPTLLARADEVIE"
     gene            848..>1700
                     /locus_tag="VM910_18050"
     CDS             848..>1700
                     /locus_tag="VM910_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64462.1"
                     /translation="MNRREFITLLGGAAAAWPLAARAQQPTMPMIGFMHILSPDNVPR
                     FVPAFRQGLKEQGFIEGQNLAVEYRWADGEYDRLPELAADLVQRGVAVLAATGGQPSP
                     NVAMRATHTIPIVFTTNGDPVSEGLVASLNRPGGNATGVTIFGPAAVTKRMQLLHELI
                     PRADETAYLMNPNNPNADPELNAAQEAARSLGMLLHVFRVSNERELDEAFATMAQQRV
                     GALVGASDTFLFGRRDHVVSLAAHYRIPAIYYLAEFAQAGGLMAYGNKITDSYRLVGM
                     YVGRILKG"
BASE COUNT          309 a          522 c          527 g          342 t
ORIGIN      
        1 gctcgcctcg cagcgttctt gcaggagctg cagcaattgg gctgggttga aggccgtggc
       61 gtccgcatcg atgttcgttg gggtgcgggc gatgccgagc gcattcgcag gtacgcgacc
      121 gaattggtcg gactgacgcc cgatatcctc atgggctcga ccactccgcc ggtggcggcg
      181 ctgcagcagg cgaccgcgac cgtgccgatc gtgttcgcgc aggttggaga tcccgtcggc
      241 gccggtttcg tcgatagtct ggcgcggccc ggcggcacca ccaccgggtt ttccgtattc
      301 gaattcagct tcggcgggaa atggctggag ctgctcaagg aaatcgcgcc gaccgtgacg
      361 cgcgtggcag tcattcggga ccccgccata gcttcgggaa tggggcagtg gggtgcgatt
      421 cagtccgcgg cgcctccgtt cgcgtcgagt tgcgtccagt caacgtgcgc gatgccgggg
      481 agatcgaacg cgccgttacg tccttcacgc gcctccctga tagcgggctg atcgtgaccg
      541 ggagcccgtt ggcatacttt catcgcgagc tgatcatcgc cctggcggcc cggtaccgac
      601 tgcctgctgt ctactcggat cgggtttacg tcgccgacgg cggcctgatc tcctacgggc
      661 ccgatcgggt cgaccagttt cggctcgcag ccggctacgt cgatcgcatc ctcaaaggcg
      721 aaaaaccagc ggacctgccg gtgcaggcgc cgaccaagta cgagcttgtg attaatctca
      781 agaccgcgaa agcgctcggc ctcgacgtgc caccgacgct gctcgcgcgt gccgacgagg
      841 tcattgaatg aaccgtcgcg agttcatcac gctgctcggc ggagcggcag cggcgtggcc
      901 actcgcggcg cgcgcgcaac agccaacgat gccgatgatc ggattcatgc acatcctgtc
      961 ccccgacaat gttccgcggt ttgtgcccgc ctttcggcaa ggtctcaagg aacaaggttt
     1021 cattgaaggc cagaacttgg cggtcgaata tcggtgggcc gacggagaat acgatcggct
     1081 gccggagctg gcagccgatc tcgttcaacg gggagttgcg gtgcttgccg caactggtgg
     1141 ccaaccatca ccgaacgtgg cgatgagggc tacgcacact attcccatcg tgtttaccac
     1201 gaacggcgat cccgtaagcg agggcttggt agcgagtctc aaccgaccgg gtggtaatgc
     1261 cacgggcgtc acaatattcg gtccagccgc ggtcactaaa cgcatgcagt tgctgcatga
     1321 gcttataccc cgcgctgacg aaacggccta tctcatgaat ccgaacaacc ccaacgctga
     1381 tcccgaatta aacgcggcgc aggaggccgc ccgttctctc ggaatgcttc tgcatgtttt
     1441 ccgcgtcagt aatgaacgtg agctcgacga agcatttgca accatggcgc aacagcgagt
     1501 gggcgccctc gtcggcgctt ctgacacttt tttgtttggc cgtcgcgacc acgtagtctc
     1561 acttgcagcc cattatcgca ttccagcaat ctactatctg gccgaatttg ctcaggctgg
     1621 aggcctcatg gcttacggca acaagatcac tgactcgtat cgcctagttg gcatgtacgt
     1681 tggacggatt ctcaagggcg
//
LOCUS       DATLJT010000798         5118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2578783,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000798 DATLJT010000000
VERSION     DATLJT010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578783"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VM910_18055"
     CDS             <1..808
                     /locus_tag="VM910_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI64463.1"
                     /translation="GPYVKKRVYDPIIQGLSGFADIQAQPVTNRPQMIRTIVCDKTTA
                     VMTAQAVAAALYAREKTGKGDHIQVAMLDVMISYLWPEGMMQYTVVGSEVGASDPTDR
                     PDLVFKTSDGYLTAGTISDSEWQGFCRASGDPELAKDPRFATPTGRSVNATARINKMQ
                     EYFSRHTTAEWLERLDAADVPCAPILRRGEIINNEQVVARGIIAEIDQPAVGRIRQPK
                     PAARFELHEAAIGGPAPRIGEHSRAVLQELGYDDRAIDKMASEKAVRLAG"
     gene            complement(879..1556)
                     /locus_tag="VM910_18060"
     CDS             complement(879..1556)
                     /locus_tag="VM910_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI64464.1"
                     /translation="MTEGPQLTLWGVGTSRTIRAHWAMFELDLPYTTKPIGPRTGETK
                     TAEYTSLNPRQKIPLLQDGDFCIGESAAIVAYLSRTYSTPARALIPEKQRDYAAWLEW
                     CFFIVTELDSTSLYVMRRHSANALGPIYGIAPDVVAKAGEYFREQLRHVEAAFNDGRT
                     YLMGDHFTSADILLTTCLDWAIGYGVGICDNAYPYLERIRKRDAYLRGTEANKPLAPI
                     TPVQAKV"
     gene            complement(1558..2301)
                     /locus_tag="VM910_18065"
     CDS             complement(1558..2301)
                     /locus_tag="VM910_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI64465.1"
                     /translation="MAEAQKVAILVGAGDAIGAAVAQRFAKGGYTVCICRRDAAKSQK
                     LVDELTSSGLKIHAFSVDARQEAEVQALFAKVENEIGPIEVCLFNAGSNVNKPLIETT
                     EKLFFKAWELACYAGFLVGREAARVMVPRGRGTILFTGATASLRGGKGFAAFASAKFG
                     LRAVAQAMARELGSKNIHVVHLIIDAAIDSEAIHARLKAASGIEAKDIAPDTLTKTSS
                     IAEAYWFAHQQSKDGWTHELDLRPAVEKW"
     gene            2583..3320
                     /locus_tag="VM910_18070"
     CDS             2583..3320
                     /locus_tag="VM910_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64466.1"
                     /translation="MQKRNATAAVIGAGDYVGGEIAKKFAAEGFTVFAGRRNGAKLEP
                     LVKSIEAADGKIHARSLDARKEEEIVSFLNEADKHAPLEVCIFNIGANVNFPILDTTE
                     RVFRKVWEMACYSGFLAGREAARLMLPRGKGNIFFTGATASLRGGKGYAAFASAKFGL
                     RAVAQAAARELGPKNIHVAHLIIDSGVDTEWVRQRRIEALGPNALDNPDLLMPPASVA
                     EAYWQLYQQPKSAWTFELEIRPFGETW"
     gene            3464..4651
                     /locus_tag="VM910_18075"
     CDS             3464..4651
                     /locus_tag="VM910_18075"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVI64467.1"
                     /translation="MTEAVIVSTARTPIGRAYKGGLNNTEGATLLGHAISAAVSRAKV
                     DAAEVEDVVMGCAMQQGTTGSNIARKALLRAGLPSSVAGTTIDRQCASGLQAIALAAR
                     SVIFDGVDIAVGGGGESISLVQNDKINTFHAVDPELLAIKGDVYMPMLDTAEIVAKRY
                     NISRELQDEYGLESQRRTASAQQAGRFNDELAPITTTMAVADKATGAISYQQVTLAID
                     EGMRPDTTAEGLATIKPAKGPGYTVTAGNASQLSDGASAAVVMSDKLASQRGLKPLGI
                     FRGFVVAGCEPDEMGVGPVFAVPRLLKRHGLKIDDIDLWELNEAFAVQVIYCRDKLGI
                     DPSKLNVNGGSIAIGHPYGMTGARLTGHALIEGRRRKAKYAVVTMCVGGGMGAAGLLE
                     IVH"
     gene            4682..>5118
                     /locus_tag="VM910_18080"
     CDS             4682..>5118
                     /locus_tag="VM910_18080"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI64468.1"
                     /translation="MKTAITELFGIQHPIIQGGMHYVGFAELAAAVSNAGGLGIITGL
                     TQKTPELLAREIARCRGMTDKPFGVNLTFLPTLSAPPYPEYIAAIREGGVKIVETAGR
                     SPEQYMPALKAAGIKVIHKCTSVRHSLKAEQIGCDAVSVDGFE"
BASE COUNT          984 a         1585 c         1636 g          913 t
ORIGIN      
        1 cggaccctat gtgaagaagc gggtctacga tcccatcatt cagggccttt caggctttgc
       61 cgacatccag gcgcagccgg tgacgaaccg cccgcagatg atccgtacca tcgtctgcga
      121 caagacgacg gcggtgatga ctgcgcaggc ggtagcggcc gcgctgtatg cccgcgagaa
      181 gaccggcaag ggcgatcaca tccaggtcgc gatgctggac gtgatgatct cctatctgtg
      241 gcccgagggc atgatgcaat acaccgtggt cgggtctgag gtgggcgcgt cggacccgac
      301 cgatcggccc gatctcgtgt tcaagaccag cgacggctat ctcacggccg gcaccatctc
      361 ggactccgaa tggcagggat tttgccgtgc gagcggcgat cccgagcttg ccaaggatcc
      421 gcggtttgcg acgcccacgg gaagaagcgt caacgctacc gcgcgcatca acaagatgca
      481 ggaatatttc tctcggcaca ccaccgccga atggctggag cggcttgatg ccgccgacgt
      541 gccctgcgcg ccaatcctgc ggcgcggcga aatcatcaac aacgagcagg tcgtggcgcg
      601 cggcatcatt gctgaaatcg atcagccggc ggtcggcagg atccgtcaac ccaaaccggc
      661 cgcacgattt gagctccatg aggctgcaat cggtgggccc gcccctcgca tcggggagca
      721 ctcgcgcgcg gtgctgcagg aactcggcta cgacgaccgc gcgatcgata agatggcgag
      781 cgagaaggcg gtccggctgg cgggctgatc atctttccgg caacggcctg cgatggtgcg
      841 acgacttcca agacacggcg tcggcgccgg aggtcttctc agactttcgc ttgcaccggc
      901 gtgatcggcg cgagcggctt attggcctca gtcccgcgca gatacgcgtc ccgcttgcgg
      961 atgcgttcga gataggggta ggcgttgtcg cagatgccga ctccataccc aatggcccag
     1021 tcaaggcagg tcgtgagcag gatgtcggcg ctggtgaagt ggtcgcccat caaataggtg
     1081 cggccgtcgt tgaacgccgc ctccacgtgc cgcagttgct cccgaaaata ttcgccggcc
     1141 ttggcaacga cgtcgggcgc gatgccgtag atcgggccaa gagcattcgc gctgtggcga
     1201 cgcatcacat aaaggctcgt tgaatcgagc tcggtgacga tgaaaaagca ccattctagc
     1261 cacgctgcat agtcgcgctg tttctcgggg atcagcgctc gcgcgggcgt cgagtaggtc
     1321 cgcgacaaat aggcgacgat cgcagcactc tcgccgatgc agaaatcgcc gtcctgcaac
     1381 agcgggattt tctgtcgtgg attgagggag gtgtattccg ccgtcttggt ctcgccggtg
     1441 cgcggaccga tcggcttggt cgtgtaaggc aggtccagct cgaacattgc ccagtgggcg
     1501 cggatggtgc ggctcgtgcc gacaccccat aacgtcagct gcgggccctc ggtcatctca
     1561 ccatttctcc actgcggggc ggaggtcgag ctcgtgggtc cagccgtcct tcgattgctg
     1621 gtgcgcgaac cagtaggcct ccgcgattga cgacgtcttg gtgagcgtat cgggcgcgat
     1681 gtccttcgcc tcgatgccgc tcgcggcctt caggcgcgcg tggattgcct cgctgtcgat
     1741 ggcggcatcg atgatcaggt gcacgacatg aatgttcttc gatcccagct cacgggccat
     1801 cgcttgcgca accgcgcgca aaccgaattt tgcggaggcg aaggccgcaa aacccttgcc
     1861 gccgcgaagg ctcgcagtag cgccggtgaa gaggatcgta ccgcggccgc gtggcaccat
     1921 cacacgcgcc gcctcgcgtc cgaccagaaa gccggcatag caggcgagct cccaggcctt
     1981 gaagaagagc ttctcggtgg tctcgatcag cggcttgttg acgttcgaac cggcgttgaa
     2041 gaggcagacc tcgatcggcc cgatctcatt ttcgactttg gcgaagagag cctgaacctc
     2101 ggcttcctgg cgagcatcga cgctgaacgc atggatcttc agacccgagg acgtgagttc
     2161 atcgacgagc ttttgcgact tggcagcgtc gcgcctgcaa atgcagaccg tgtaaccgcc
     2221 cttggcaaaa cgctgtgcca ccgcggcgcc gatcgcatcg cctgcgccga caagaatggc
     2281 caccttttgc gcttccgcca tggcttcctc tataagttct aattttgtac tttactctta
     2341 gccggagtag cggtccttgt aaatcggctt tttgatccgg gttgcgacaa aatgggcgtc
     2401 ggttattcaa atttgagttg gctcgtcgga atctttttcg ccatcggttg actagttcta
     2461 aaaaagaact taaagactat ggcggcgatg gacctggtca agggcggcaa atgccgctcg
     2521 gcggaatcct ggcggtcctt ggatccgctg gtttcgcgac atcgcgggat gaggagatgc
     2581 ctttgcagaa gagaaacgcg accgcggcgg ttatcggcgc cggcgactac gtcggcggcg
     2641 agatcgcaaa gaagtttgca gccgagggtt tcacggtctt cgcggggcgc cgcaacggcg
     2701 ccaagctcga gccgctggtg aaaagcatcg aggcggcgga cggcaagatc cacgcgcgct
     2761 cgctggacgc ccgcaaggag gaggagatcg tctccttcct taatgaagcc gacaagcacg
     2821 cgccgcttga ggtgtgcatc ttcaacatcg gcgccaacgt caatttcccg atcctggata
     2881 cgaccgagcg cgtctttcgc aaggtctggg agatggcctg ctattccggc ttcctcgccg
     2941 gacgcgaagc ggcgcggctg atgctgccgc gcggcaaggg caacatcttc ttcaccggcg
     3001 cgaccgcgag cctgcggggc ggcaagggct atgccgcttt tgccagcgcc aaattcggac
     3061 tgcgcgccgt ggcgcaggcg gccgcgcgcg aactcgggcc gaagaacatc catgtcgcgc
     3121 atctgatcat cgattccggt gtcgatacgg agtgggtgcg ccagcgccgg attgaggcat
     3181 tgggtccgaa tgccctcgac aatccggatc tgctgatgcc gccggcctcg gtcgccgagg
     3241 cctactggca gctttaccag cagccgaaaa gcgcctggac ctttgaactc gaaatccgcc
     3301 ccttcggcga gacctggtga cggcgatagc gatgagcgtc cgccatccat cggggtcggc
     3361 gatcccggct ttcacctgac gcgccggacc cgcgcggatc tgcgaaccgc ggccttaaac
     3421 agcattgttg ccaatccaat cagacagaga gagagcaaga caaatgaccg aagctgtgat
     3481 cgtctcgacg gcccgtaccc cgatcggcag ggcctacaag ggcggactga acaataccga
     3541 aggtgcgacg ctgctgggtc acgccatctc ggctgcggtt tcgcgcgcca aggtggatgc
     3601 cgcagaggtc gaggacgtcg tgatgggttg cgccatgcag caaggcacga cgggaagcaa
     3661 cattgcgcgc aaggcgctgt tgcgcgccgg acttccctcg tcggtggccg gaaccaccat
     3721 cgaccggcaa tgcgcttcgg gcctccaggc gattgcgctc gccgcgcgtt cggtgatttt
     3781 cgacggcgtc gacattgccg tcggcggtgg cggtgaatcc atcagcctcg tccagaacga
     3841 caagatcaac accttccacg ctgtcgatcc ggagcttctt gcaatcaagg gcgacgtcta
     3901 catgccgatg ctcgacactg cggagatcgt cgccaagcgc tacaacatct cccgcgagct
     3961 ccaggacgaa tacggcctgg aaagccagcg tcgcacggca tctgcccagc aggcgggccg
     4021 tttcaacgac gaactcgcgc cgatcacgac gaccatggcg gtcgcagaca aagcgacagg
     4081 cgcgatcagc tatcagcagg tcacgctcgc aatcgacgaa ggcatgcggc cggacaccac
     4141 ggcggagggc ctggcgacga tcaagccggc aaagggaccc ggctacaccg tcaccgccgg
     4201 caacgcgagc cagctctcgg atggcgcaag tgccgccgtc gtgatgagcg acaagctcgc
     4261 ctcgcagcgc gggctgaagc cgctcggaat tttccgcggc ttcgtcgtgg ccggctgcga
     4321 gccggatgaa atgggcgtcg gcccggtctt cgccgtgccg cgcctgctca agcggcatgg
     4381 tctgaagatc gacgacatcg acctctggga actgaacgaa gcgttcgcgg tgcaggtgat
     4441 ctactgccgc gacaagctcg gcatcgatcc gagcaagctc aacgtcaacg gcggctcgat
     4501 cgccatcggc catccctatg gcatgacggg cgcacggttg accggccatg ccttgatcga
     4561 gggccgccgg cgcaaggcga agtacgccgt cgtcaccatg tgcgtcggcg gcggcatggg
     4621 cgcggccggt ctcctcgaaa tcgtgcattg atcccaagag ccggcagggc aggagcctct
     4681 catgaagaca gcgattaccg agctattcgg catccagcat ccgatcatcc agggcggaat
     4741 gcactacgtc ggcttcgcag agcttgcagc ggcagtttca aatgctggag ggctcggcat
     4801 catcaccggc ctcacgcaga agacgcctga actgctcgcc agggagatcg cgcgttgccg
     4861 cggcatgacc gacaagccgt tcggcgtgaa cctcaccttc ctgccgacgc tgagcgcgcc
     4921 gccctatccg gaatatatcg ccgcgatcag ggaaggcggg gtcaagatcg tcgaaaccgc
     4981 cggacgcagt cccgagcaat acatgccggc gctgaaggcg gccggcatca aggtgattca
     5041 caagtgcaca tcggtccggc attcgctgaa ggccgagcag atcggctgcg acgccgtcag
     5101 cgtggacggt ttcgaatg
//
LOCUS       DATLJT010000799         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1889095,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000799 DATLJT010000000
VERSION     DATLJT010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889095"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..291
                     /locus_tag="VM910_18085"
                     /pseudo
     CDS             10..291
                     /locus_tag="VM910_18085"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
     gene            complement(399..1547)
                     /locus_tag="VM910_18090"
     CDS             complement(399..1547)
                     /locus_tag="VM910_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64469.1"
                     /translation="MSLPAEGKLSAPPATASRANGAADIAVELDGVTKRFGKITALDD
                     VSLLIRRGELITLLGPSGCGKTTLLNVVAGFLMPDSGEIAVDGQRITDVPAYRREIGI
                     MFQNYALFPHMNVAANVGYGLRMRRVAKAEIARRVADALALIKLAGLEDRKPRQLSGG
                     QQQRVALARALVIRPKVLLLDEPFSALDRNLRTSMQVELKEIQRKLGVTTIFVTHDQS
                     EALSLSDRIAVVAEGRIRQLGTPDEIYRRPVDRFVASFVGDVNVLHARLERIDAAVAT
                     VALGAARVLVPSRTLQGAGTGSMVDLFVRPEELRPAEPGAAVATHGIVAAQIYQGAHV
                     DLYVDAPEALSGRVLLRVPRHEGMSLWPAGTRISIALAADEAIAFKSA"
     gene            complement(1531..2349)
                     /locus_tag="VM910_18095"
     CDS             complement(1531..2349)
                     /locus_tag="VM910_18095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64470.1"
                     /translation="MPRLPSQPLLRLLGGVTVGAILGFLLLPALVVTLAAFNSRAILS
                     FPPESWSWRWFVKALAYEDFQAGFHNGLIVTGWASSIALVVGAAFALALERYEFRFKR
                     ALETVLLSPLVVPHFTVGLGFLILAAQLGLARGYAVIIACHVVLVLPFVLRSVYISLR
                     NLDPRLELAAASLGAPPHRVLTTVTLPLLLPGLVSGWLFAAILSFNEFTASLFVTVQR
                     TQTLPVALYNYVREYADPSMAAISVMYIVVTATLLTLANVFLGLAKVLNVEPPR"
     gene            complement(2351..3223)
                     /locus_tag="VM910_18100"
     CDS             complement(2351..3223)
                     /locus_tag="VM910_18100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64471.1"
                     /translation="MSSRRAVMTPRPFLRAFVAPATLCAFAFVAALAAVMQYSLRAYV
                     PGSLEPGGFTLANFTALLKPLYARVFLDTVWICFLTALFTLIIGYPLAYALVHARNVA
                     LKSVILVIAVTPLFLGEVVRTYSWIVVLGNNGFVNSMLLKAGLIGAPVQFMFTEFGVV
                     TALVHVTLPVMVIMLAAALSHIDRDYERAAASLGAGPIRVFLTVTLPLSTPGIIAGVT
                     TAFAWTFSAFATPQLIGGGRVNMVANLVYQLGFASFNFPFAASLSVAGLVLTFAVLAL
                     MRAAAGPLEKIGLH"
BASE COUNT          662 a         1097 c         1153 g          481 t
ORIGIN      
        1 accgagtttc tggggcccaa cccggtcggc tacatcaact acggcgccga cgggcgcatg
       61 ctcgtgctca ccgtggggag cggccgccgc aagcccgccg gaccgaaggc gacctccgcg
      121 gaggtggaag cgctgtttcg cagcatgacc agctacggcg gcacctatac catcgacggc
      181 aacgagatca cccatcacgt cgacgtctcc tggaaccaga gctggaccgg caccgatcag
      241 aagcgcgccg cccgtttcga cggcgatcgc gtctatctct cgacgccgcc ttcgctcgat
      301 ccgatcaccg gcacgatgag cgtgcgcacc atacctggga gaagctgggt tgagcgcctc
      361 ctgcggcgga cgcctcgtcc ggatcgcacg acggccgctc acgccgactt gaaggcgatc
      421 gcctcgtccg cagcaagcgc gatgctgatg cgcgtgccgg cgggccaaag cgacatccct
      481 tcgtgccggg gcacgcgcaa cagcacgcgt cccgacaggg cctccggcgc atcgacgtag
      541 agatcgacat gggcgccttg atagatttgc gccgccacga tgccgtgcgt cgcgaccgcc
      601 gcgccgggct cggccgggcg caattcctcg ggtcgaacga ataggtcgac catggaaccg
      661 gtgccggcgc cttgcagcgt gcggctcggc accagcaccc gcgccgcgcc aagggcgacc
      721 gtggcgaccg ccgcgtcgat gcgttcgagg cgcgcgtgca gcacgttgac gtcgccgacg
      781 aaggacgcga cgaaacggtc gaccgggcgg cgatagatct cgtccggggt gccgagttgg
      841 cggatgcggc cctccgcgac cacggcaatc cggtccgaca agctgagcgc ctcgctttgg
      901 tcgtgcgtga cgaagatggt cgtcacgccg agcttgcgct gaatttcctt gagctcgacc
      961 tgcatcgagg tgcgcaagtt cctatcgagc gcggagaacg gctcatcgag cagcagcacc
     1021 ttgggacgaa tgacgagcgc gcgcgcgagc gccacgcgct gctgctgacc gcccgagagc
     1081 tggcggggtt tgcgatcttc caggcccgca agcttgatga gcgcgagcgc gtcggcgacc
     1141 cggcgcgcta tctccgcctt ggcgacccgg cgcatgcgca ggccgtagcc gacattcgcc
     1201 gcaacgttca tgtgggggaa cagcgcgtag ttctggaaca tgatgccgat ctcgcgccga
     1261 tacgccggga catccgtgat ccgctggccg tcgacggcga tctcgccgct gtccggcatc
     1321 aaaaagccgg cgacgacgtt gagcagcgtg gtcttgccgc agccggatgg cccgagcagc
     1381 gttatcagct cgccgcgacg gatcagcagc gagacatcat cgagcgcggt aatcttgccg
     1441 aagcgtttgg tcaccccgtc gagctcgacc gcgatatccg cggcgccgtt cgcccgcgac
     1501 gccgtcgcgg gcggagcgga tagcttgcct tcagcgggga ggctcaacgt tgagcacctt
     1561 tgcaaggcca aggaagacat tggccagcgt cagcagcgtt gcggtgacca caatgtacat
     1621 gaccgagatc gccgccatcg aggggtcggc gtattcgcgc acgtagttgt agagcgcaac
     1681 cggcagcgtt tgagtgcgct gcacggtgac gaacagcgag gcggtaaatt cgttgaacga
     1741 caggattgcc gcgaacaacc agccgctgac caagcctggg agcagcaacg gcaaggtaac
     1801 ggtggtgaga acgcggtggg gcggtgcgcc gaggctggcg gcggcgagct ccagtcgcgg
     1861 atcgagattg cgcagcgaga tgtagacgct ccgcaacacg aacggcagca cgagcacgac
     1921 gtggcaggcg atgatcaccg cgtagccgcg ggccaagccg agctgcgccg cgaggatcag
     1981 aaaaccgagg ccgaccgtga agtggggcac caccagcgga gagagcagca cggtctcgag
     2041 cgcccgcttg aaacgaaact cgtagcgctc gagcgcaagc gcgaacgccg cgccgaccac
     2101 aagcgcgatc gaggaggccc agccggtcac gataagcccg ttatgaaagc cggcttgaaa
     2161 gtcttcgtac gcgagcgcct tgacgaacca gcgccacgac cagctctcgg gcgggaacga
     2221 cagtatggcc ctgctgttga aggcggcaag ggtgacgacc aacgccggca atagcaagaa
     2281 gccgaggatg gcgcccacgg tgaccccacc cagcaggcgc aggagtggct gcgacggcaa
     2341 gcgcggcatc ttagtgcagt ccaatttttt cgagcgggcc ggcggcggcg cgcatcagcg
     2401 cgagcaccgc gaatgtgagc acgagccccg cgaccgacag gctcgccgcg aacgggaagt
     2461 tgaagctcgc gaatccgagc tgatagacca ggttcgcgac catgttgacg cgaccgccgc
     2521 cgatcagctg cggggtcgcg aacgcgctga aggtccaggc gaacgccgtg gtgacgccgg
     2581 cgatgattcc gggcgtcgag agcggaagcg tgacggtgag aaagacgcgg atcgggccgg
     2641 caccaaggct cgcggccgcg cgctcgtagt cgcggtcgat gtgcgagagc gccgccgcca
     2701 acatgatcac catcaccggc aaggtgacgt ggacgagcgc ggtgacgacg ccgaactcgg
     2761 tgaacatgaa ttgcacgggc gctccgatca gcccggcctt gagcagcatc gagttcacaa
     2821 atccgttgtt gccgagcacg acgatccacg aataggtgcg gaccacctcg ccgaggaaca
     2881 gcggggtgac ggcgatcacc aaaattacgg acttgagcgc gacgtttctg gcgtgcacca
     2941 gcgcataagc gagcgggtat ccgatgatga gcgtgaacaa tgccgtgagg aagcagatcc
     3001 aaaccgtgtc gagaaacacc cgcgcgtaga gcggcttcag cagcgccgtg aagtttgcga
     3061 gcgtgaagcc ccccggctcc agcgaacccg gcacataggc acgcaggcta tattgcatca
     3121 ccgccgccag cgcggccacg aaggcgaacg cgcacagcgt ggccggcgcc acgaaggcac
     3181 gcaggaacgg cctcggcgtc atgaccgccc ggcgggatga cacggcgcgc ccgcgccgca
     3241 cgcggccatc aggccgtcag ttcatgatgt tctcggcaaa ccacttgcgc cactccgcgg
     3301 tcttctccgc gcgcagcttg tgatcgatga tgatggcctg cttgttccac tggtcggccg
     3361 aggtgaacac tcccggcagc ttggcgatgg ccg
//
LOCUS       DATLJT010000800         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3726856,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000800 DATLJT010000000
VERSION     DATLJT010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3726856"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VM910_18105"
     CDS             <1..911
                     /locus_tag="VM910_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HVI64472.1"
                     /translation="VDVMLMQALMKAVPDKAALLIVGDIDQLPSVGPGQVLADIISSG
                     AVPVVRLTEVFRQAAQSRIITSAHRINQGFIPELSSPSQSDFYFVQADDPEIAVPRII
                     ELVKTRIPKRFGLDPVRDIQVLCPMNRGGVGARSLNIELQAALNPAGERKVERFGWTF
                     APGDKVMQIENDYAKDVYNGDIGYIDDVNPDDDELTASFDGRPVTYGFGELDTLVPAY
                     AVTIHKSQGSEYPAVVIPVMTQHYAMLQRNLLYTGVTRGKKLVVLVGQKKAVAIAVGN
                     VSGRRRWSKLNEWLRPVLRGDSTTRG"
     gene            complement(1353..1522)
                     /locus_tag="VM910_18110"
                     /pseudo
     CDS             complement(1353..1522)
                     /locus_tag="VM910_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050995.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(1604..>2782)
                     /locus_tag="VM910_18115"
     CDS             complement(1604..>2782)
                     /locus_tag="VM910_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVI64473.1"
                     /translation="AKTIAAEKPQAPQFQNRPLKVFGEHDHATVAQMRNCMSVGNVVA
                     GVVCADGHLGYAQPVGGVIAYEKQISISGVGFDIGCGNMAIRLDTPFNAVEDRVCTII
                     KDVSRTISFGVGRTNEEQVDHELFDDAEAWAASDMESYRQKAAAQLGTVGSGNHYVDL
                     LRDEDGFVWIGVHFGSRGLGHTSATRYLKAAGGKSGINVPPALIDEDSELGRRYMAAM
                     ELAGRYAYAGREWVVERVRRLIGGAVTDTVHNHHNYAWRETHEGRDVWVVRKGATPAF
                     PGQRGFVGGSMGDDAMIIEGIESPQARDSLYSTVHGAGRLFGRKEAKRRFTREQMNRW
                     LQDRGVTLIGADLDESPMAYRRLPEVIAQHAGTVKVLHTLRPFAVAMAGTRELDPWKD
                     "
BASE COUNT          534 a          896 c          808 g          544 t
ORIGIN      
        1 tggtcgacgt catgctgatg caggcgctca tgaaggcagt gcccgacaaa gccgcccttt
       61 tgatcgttgg cgacatcgac caacttcctt cggtcgggcc tggccaagtg ctggccgaca
      121 tcatctcatc tggggctgtg ccggtggtgc gcctgaccga ggtgttccgg caggccgcgc
      181 aaagccgcat catcaccagc gcccaccgca tcaaccaggg cttcattccc gaactcagct
      241 ctccctcgca gagtgatttc tacttcgtgc aagcggacga tcctgaaatc gccgtgccgc
      301 gcatcatcga gttggtgaaa acacggattc ccaaacgttt cggtctcgat cccgtccgcg
      361 acatccaggt tctttgcccg atgaatcggg gcggcgtcgg cgcacgctcc ctcaacatcg
      421 agctacaagc agcattaaac cccgcgggcg agcgtaaggt cgagcgcttc gggtggacct
      481 tcgctcccgg cgataaggtg atgcaaattg agaacgacta cgcaaaggat gtttataacg
      541 gtgacatcgg ctatatcgac gatgtcaatc ctgacgatga cgaactcacc gccagcttcg
      601 acgggcgccc cgtgacctac ggatttggcg aactcgacac gcttgtgccg gcctacgccg
      661 tgaccatcca taaaagccag ggttcggagt atccggccgt ggtcatcccg gtgatgacgc
      721 agcactacgc catgctgcaa cgaaatctac tgtataccgg cgtcacgcga ggcaaaaagc
      781 tggttgtgct ggtcggtcag aagaaggcgg tagccattgc ggttggcaac gtttcgggtc
      841 ggcggcggtg gtcgaaattg aacgagtggc tgcgccccgt gttacgcggc gactctacga
      901 cacgaggttg actaagccgt ccagcacgtt ggcgagaaga aaaattctcc ggatcgcggc
      961 aatagtatcc gcatgattgc gcaagaatgc ccgccgggtc tgcgatggcg gtccgcggcg
     1021 tcggatcatg tacttggaga ccgtcgattc cgccacctcg aacccgagtt tcagtaattc
     1081 gccatgaata cgcagagcgc cccacaaggg gttctcatcg cagcccgatg ccagcgcaga
     1141 atggctgcac aacttgagcc aaatcgagca gattttgcca aagccgtgtt attaccaatc
     1201 ccctgagtgg ctgactctct agggaattcc ttgcggccat agacaatgat gtcgagcggc
     1261 tcccccttgg ccttccaacc ttgctgatga ccgctggcgt ctcgtaattg ccgaaccagc
     1321 gccctcctgt agcggtcagc cgtttatctc gcgccgtaaa agtcgccggt atccggcatg
     1381 gtgatgccgg cctcgagcgc ggcatggaag gccgcgatgg agtccgcgtc gccagcgtgc
     1441 ccatagccgc ccacatgccc atgcaaccga gccccatttc ggaaacgccg ggaccgtttc
     1501 gtcccaatcg aacaagccgc atctcaatct ccccggattt tcgggcgcgc aattctcaac
     1561 aaatggtcag cgaatttgtc gcacgccatg taagccccat gtactagtcc ttccagggat
     1621 ctagctctcg cgtccccgcc atcgccactg caaacggccg cagggtgtgc agcaccttca
     1681 cagtcccggc gtgttgcgcg atcacttctg gcaggcggcg gtaagccatc gggctttcgt
     1741 ccaggtcggc gccgatcagc gtcacgccgc ggtcttgcag ccaccgattc atttgctcgc
     1801 gggtgaaacg tcgcttggcc tccttgcgcc cgaaaagccg gccggcgcca tggacggtcg
     1861 agtagagcga gtcgcgcgcc tgcgggctct cgatgccttc gatgatcatg gcgtcgtcgc
     1921 ccatcgaacc gccgacaaag ccacgctgac cgggaaacgc cggcgtcgcg cccttgcgca
     1981 caacccatac atcgcgcccc tcgtgggtct cgcgccaggc atagttatgg tggttgtgca
     2041 ctgtgtcggt cacggcgccg ccgatgagtc gccgcacccg ctccacaacc cactcgcgac
     2101 ccgcgtaggc ataacgtccc gcgagctcca tcgctgccat gtaccggcgg cccaattcgc
     2161 tgtcctcgtc aatcaaggcg ggcggaacgt tgatgccgct tttgccgccg gccgctttga
     2221 gataccgggt cgcgctcgtg tgcccaagtc cgcggctacc gaaatgaacg ccgatccaga
     2281 cgaaaccatc ctcgtcgcgc aggagatcga cgtagtgatt cccggacccg acggtgccga
     2341 gctgcgccgc cgccttctgg cgatatgatt ccatgtccga tgccgcccag gcttcggcat
     2401 cgtcgaagag ctcgtggtcg acctgctctt cgttggtgcg accgacacca aacgaaatcg
     2461 tgcgggacac gtccttgatg atcgtgcaaa cgcggtcctc gaccgcgtta aacggcgtat
     2521 cgagacggat ggccatgttc ccgcagccga tgtcgaagcc aacgccggaa atgctgatct
     2581 gcttctcgta ggcaatgacg ccaccgaccg gttgcgcgta accgagatga ccatcggcgc
     2641 acacgacgcc ggcgacgacg ttgccgaccg acatgcaatt gcgcatctgc gcaaccgtcg
     2701 cgtggtcgtg ctcgccgaac accttgagcg gcctgttctg gaattggggg gcctgcggct
     2761 tttcggcggc gatcgttttc gc
//
LOCUS       DATLJT010000801         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4186758,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000801 DATLJT010000000
VERSION     DATLJT010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4186758"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VM910_18120"
     CDS             <1..924
                     /locus_tag="VM910_18120"
                     /inference="COORDINATES: protein motif:HMM:NF021541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HVI64474.1"
                     /translation="FNGSRVDRAGRIVSFGHSEVESDQEIDGNVSARQIPWRQVLGYW
                     EKLNDRKIGEGAGGALQVWYYPGALSDDPPSVLHRKKIQLRRLLKFISGLDLSQMGPE
                     SDDLREALQQSVVRSSISDVPWSAAFVSSIMRAVPLDEDTEFSYDASHVAYITSAVEQ
                     SQRDLVGGDSASFYRACDPDTTKPRTGDLFCYHRHIEGTPHPYAQKGPSLFKSLFRDI
                     ARGEEPISRSHCDIVVSVDSDSSKVTVIGGNVQNSVTEKVLNLNKKGVLSAAQGSTAC
                     DSYNPDKPTSGSEPNCNPNRQKWFVLLQAAI"
     gene            complement(942..>1854)
                     /locus_tag="VM910_18125"
     CDS             complement(942..>1854)
                     /locus_tag="VM910_18125"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVI64475.1"
                     /translation="YAGVAVAFNKGMADSPKPKVMVALGDTWPLPPDIVQAVNESPAV
                     FSRSSDRMLRAITLYTRYGRSLARTRLPADPAPVAGMPRLGKGTQPEWLSKKILAAAG
                     IRVPDGELARGPDHAVAIATRVGYPVALKAQAAALPHKTEAGGVMLNLGDETALRAAW
                     ETTQQNVERAAPGVTLDGCLVETMSPKGLELMIGAKRDPAWGTVLLVGLGGIWVEALG
                     DVQVVPVDADKTQIVEALHELRAAKLLTGFRGAPPVDVEAVTKAVLAIGRLMRTTPGI
                     VEIDVNPLMVHAKGQGVTALDALIVAE"
BASE COUNT          371 a          566 c          560 g          357 t
ORIGIN      
        1 ttcaatggaa gccgtgtcga ccgggcagga cgcatagtct cttttgggca ctcggaagtg
       61 gagagcgacc aggaaataga tggcaacgtt tctgcgagac agatcccatg gcgtcaggtt
      121 ttgggatatt gggaaaaact caatgaccgg aaaatagggg aaggcgccgg gggcgcgctt
      181 caagtgtggt actatcctgg tgctctgagt gatgatccgc cgtccgttct gcatcggaaa
      241 aaaatacagc tacggcgtct tttgaaattc atatcaggac tcgatctgtc tcaaatgggg
      301 cccgagtccg acgacctacg agaggctctg caacaaagcg tagtccgcag tagtatcagc
      361 gacgttccgt ggtcagccgc cttcgtatct tccattatgc gcgctgttcc cctcgacgag
      421 gataccgagt tctcctatga cgccagtcac gtggcctata tcacctccgc cgttgaacaa
      481 tcccagagag atttggtcgg aggggacagc gccagctttt accgtgcttg cgatcctgac
      541 acaaccaaac ctcggactgg tgatctattt tgctatcacc ggcacatcga aggtacacca
      601 cacccgtacg ctcaaaaggg gccgtccctg ttcaagtcgc tcttccgcga catagcgaga
      661 ggtgaggaac ccatcagccg tagtcactgc gacatcgttg tcagcgtcga cagcgattcc
      721 agtaaagtca ctgtgatagg aggcaacgtc cagaattccg tgaccgaaaa ggtactcaat
      781 ctaaataaaa aaggcgttct ttctgcagct cagggaagca ctgcgtgcga tagctacaac
      841 cccgataaac ccacatcggg tagcgagccc aattgcaatc cgaataggca gaagtggttc
      901 gtgctcctac aggcagcgat ctgatagacg gcgcgtctct ttcattcggc aacgatcaga
      961 gcatcgagcg cggtcacgcc ttggcctttg gcgtggacca tcaacgggtt gacatcgatc
     1021 tcgacaatgc cgggcgtggt ccgcatcagc cggccgatcg cgaggacagc cttcgtcacc
     1081 gcttcgacgt cgacgggtgg cgcgccgcgg aatccggtga gcagctttgc cgcgcgcagc
     1141 tcgtgcagcg cctcgacgat ctgcgttttg tcggcgtcga ccggaacgac ctgcacgtcg
     1201 cccagcgcct cgacccagat gccgccaagt ccgaccagca gcaccgtccc ccaggcggga
     1261 tcgcgtttgg ccccaatcat cagttcgagg cccttgggcg acatggtctc gacgaggcag
     1321 ccgtcgagcg tcacgccggg cgcggcgcgt tcgacgtttt gttgcgtcgt ctcccaggcc
     1381 gcacgcagcg ccgtctcgtc gcccaaattg agcatgacgc cgccggcttc ggtcttgtgc
     1441 ggcagcgccg cggcctgtgc tttcaaggcc accgggtagc cgacgcgcgt ggcgatagcg
     1501 acggcgtggt ccggcccgcg ggcaagctcg ccatcgggca cgcggatgcc ggccgcggcg
     1561 aggattttct tgctcaacca ctccggctgc gtgcccttgc cgagcctcgg catgcctgca
     1621 accggagcgg gatcggcggg caggcgcgtg cgcgcgagcg agcgcccata ccgtgtatag
     1681 agcgtgatcg cgcgcagcat gcggtccgac gatcgcgaaa acaccgcggg actttcgttg
     1741 acggcctgca cgatgtcggg agggagcggc caggtatcgc cgagcgccac catcaccttg
     1801 ggcttgggtg aatccgccat gcccttgttg aaagccactg ccaccccggc gtag
//
LOCUS       DATLJT010000802         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3727250,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000802 DATLJT010000000
VERSION     DATLJT010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727250"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VM910_18130"
     CDS             <1..622
                     /locus_tag="VM910_18130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVI64476.1"
                     /translation="ERHVADLWAANSLDCEARMWTYLPWGPFRSFDEYLAYMQTVAPK
                     EDPLIHAVIDIDTDKAVGVASYLRVNADAGTIEVGGLAYSPLLQRRPAATEAMYLMMR
                     RVFDELGYRRYEWKCNALNAPSRAAATRLGFQFEGIFRQADVVKGRNRDTAWFAIIDR
                     EWPALRAVFERWLDPGNFDESGRQRVSLSSLISGLGESSNSQAYAK"
     gene            complement(1022..1888)
                     /locus_tag="VM910_18135"
     CDS             complement(1022..1888)
                     /locus_tag="VM910_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI64477.1"
                     /translation="MKTVEIIYRYRSPSAPTRPRPRDAEAARLRLSDGNHAFATLLDG
                     LSQGAGRARRVIDIDPRDLGLLEEHSAPPQHPFAVVLGCSDARVPIELIFNEGPNDLF
                     VVRVAGNGLGHDVLGSLRYALEHLRESLRLVVILGHSGCGAVSAAVDAFLHPLGYLSV
                     STSYSLRGILDPLLVVVEAAARELGATYGSDVAEQPGYRDALIAASVASNAAQVAFTL
                     QRELTGLGLNELRVVYGVYRLETREVWIPPGTADAATRLAHPPTDPVAFGQLGDAIAK
                     SDLIARDLSRQE"
     gene            2156..2395
                     /locus_tag="VM910_18140"
     CDS             2156..2395
                     /locus_tag="VM910_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64478.1"
                     /translation="MKAYTGPPMTLGNAAIAGARLVVWCKACHYQAEPDPGEMAEPFG
                     AETTVPDWRDRLICLQCGSCDIIDMVVTGTKRRPG"
     gene            complement(2501..2686)
                     /locus_tag="VM910_18145"
     CDS             complement(2501..2686)
                     /locus_tag="VM910_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64479.1"
                     /translation="MGAESGALQTISQMIYTGSYALAYGVVYAAVFVTQFLPQENPVM
                     RGFHDGGQAAMNELSRS"
BASE COUNT          625 a          997 c          985 g          589 t
ORIGIN      
        1 ggaacggcac gtcgctgacc tgtgggcggc caatagcctc gactgcgagg cacggatgtg
       61 gacctacctg ccgtggggac cgttccgcag cttcgacgaa tatctcgcct acatgcagac
      121 cgtcgctcca aaggaagacc cgctgatcca cgccgtcatc gacattgaca ccgacaaggc
      181 ggtcggcgtc gcgagctatc tacgcgtcaa cgcagatgcc gggacgatcg aggtcggcgg
      241 cctcgcctat tcgccgttgt tgcaacggcg gccagctgct accgaggcca tgtatctgat
      301 gatgcggcgg gtattcgacg agctcggtta tcgccgctat gagtggaaat gcaacgcgtt
      361 gaacgcaccg tcgcgggccg ccgcgacccg gcttgggttt cagttcgaag gaatctttcg
      421 ccaggccgat gtcgtcaagg gccgcaaccg cgacacagcg tggtttgcga ttatcgaccg
      481 agaatggccg gcattgcgcg cggtgttcga gcgctggctc gatcccggca atttcgacga
      541 gagtggccgg cagcgcgtca gcttgtcgtc attgatcagc ggattgggtg agagcagcaa
      601 ttcccaagcc tatgccaagt aattggcaca tcaaccgcat gtggatggcc gtgcatctcg
      661 cccgtcgctc cgcgtccgcg cgaaggtccc ctgactgaac cgatagcgga cgctcaacct
      721 cggccgcagg aacgcggcct gatgccccga agccgtcatt aagggtacgg ccaaggatca
      781 cccacgatgg gaaatcttac catcatcgtg acgagcaccc ctactgaagg gcttcgcggt
      841 gttcggccaa gcggctttct cattcgccag ctatacgacg gcacctgcaa tccggcgcca
      901 tgtcgccgcg ccgttgtgat cgccggcgga tgcgaactcg ccggctagcg gcggttccct
      961 ctcgccattc aatcccacgg ctttgagctt tgatccctgc actgttccgc cctacccatg
     1021 tttattcctg tcggctgaga tcccgggcga tcaggtctga tttggcgata gcgtcaccaa
     1081 gctgaccgaa ggcgacaggg tcggttggcg gatgggcgag ccgcgtcgcg gcatccgcgg
     1141 tgccaggcgg gatccagacc tcgcgcgtct caagtcggta gacgccgtag acgacacgca
     1201 gctcgtttag cccaagaccg gtcagctcac gctggagggt aaaggcaacc tgagcggcgt
     1261 tgctggcaac cgaggccgca ataagcgcgt cgcgataccc cggctgttcc gcgacatcgc
     1321 tgccataggt ggcgccgagt tcgcgtgccg ctgcttcgac cacgaccaag agcgggtcga
     1381 gaataccgcg aagcgagtaa ctcgttgata ccgacaggta gccgagcggg tgaagaaagg
     1441 catcgaccgc cgccgacacc gcaccgcacc cgctatggcc cagaatcaca acgagccgca
     1501 ggctttcgcg cagatgttca agcgcatacc tcagactgcc gagaacatcg tggccaaggc
     1561 cgttgccggc aacccggacg acaaacaggt cgtttggtcc ctcgttaaag atcagttcga
     1621 tcggtacccg tgcgtccgaa cacccgagca cgaccgcaaa agggtgctgc ggcggtgcgc
     1681 tgtgctcctc cagcaagccg agatcccgcg gatcgatgtc gatcacacgc cttgcccgcc
     1741 cggcgccctg ggacaagccg tcgagcaatg tcgcgaaggc atgattaccg tcgctgagac
     1801 gcagccgggc cgcctccgcg tcgcgtggcc gagggcgtgt cggcgcactg ggagaccggt
     1861 atcggtagat aatttcgacg gtcttcatcc gtatctcccg gaagaggacg cacgagcgct
     1921 agtatggcac ttaggaatcc tgatttgacc gacgacgaga agcttgcccg cgcggcacag
     1981 ttgaaacgtg caatcgctgc ggaccgttat ccactctccc ctcgggttca gacgctcaag
     2041 gcgattttgg tgaaactcga gcgcgaagca attggggtgc gggagctgct tagaactgcg
     2101 gcgccaggga ccgtccccgc gcagctctgg cctcgatgaa gcggcggcga cgggggtgaa
     2161 agcctacacc ggcccgccaa tgaccctggg caacgccgcg atcgcgggcg cccgattggt
     2221 ggtgtggtgt aaagcttgcc actaccaagc tgagcccgac cccggcgaga tggccgagcc
     2281 cttcggcgcc gagacgactg ttcctgattg gcgcgatcgg ctcatatgct tgcaatgcgg
     2341 tagttgtgac atcatcgaca tggttgtgac cggcaccaag cgccgccctg ggtagcctgg
     2401 tggagccaac caggccacct ttgccgccgg accgatctga gcgagcgccg gtcgccaaaa
     2461 tcaatgctgt ctctaatgga gcaatgcagc tcgatctgac tcaacttcgg ctgagttcgt
     2521 tcatcgccgc ttgcccaccg tcgtggaacc cgcgcatcac cgggttctct tggggcagaa
     2581 actgagtaac gaacactgca gcgtagacca cgccatatgc tagcgcgtac gacccagtgt
     2641 agatcatctg cgagatcgtc tgcagcgcgc ctgactcggc tcccaccgcc tcctccgtcg
     2701 gggtgatgtg ctccagagcc gttcctgcct cgtttctcac gattgaagaa ctggacctcc
     2761 tagtggttgt tgctgccatc tctatcgtcc tgcacaaccc actcctcaat gtaccagtct
     2821 aggagacgag catgtgtgta tcaaaccgaa gttggcagca agctgataag gcagacggtg
     2881 gatagcccgt caaacagacg gcatcatggc gcagtttgtg gagggaaccc tctcagctta
     2941 ctggcaagcc aagcagctga gcgcgaccga tcggtttcat gcatgcgtcg ggggacagtt
     3001 cccggtcatc ccgggacgcg tcagttgctc cattcgcgac acggcctgca tccggcagcc
     3061 atgcggcccg caccgcaatg tccgcgaaaa tcctatactg cagatgggga tcagaatgga
     3121 ggctggtgaa tgggtgcagg gccgttggtc gtgacctatc cgctgacgcg gcgcagccgg
     3181 gcgatcgtcg aggaag
//
LOCUS       DATLJT010000803         8387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3957059,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000803 DATLJT010000000
VERSION     DATLJT010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957059"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1162)
                     /locus_tag="VM910_18150"
     CDS             complement(113..1162)
                     /locus_tag="VM910_18150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI64480.1"
                     /translation="MSKPLVRELDSRLGFLGQFSAVDQVQLRAQVGGTLTGIHFKDGD
                     IVHQGDLLFTIDPRPYEIRLAQATAQAESASARLELAKPELWRAQELKRTDFGTMQAV
                     DQRTADLQAAQASVDDAKAQIRDAQFDLDHCRIIAPFTGRIGTHLVSVGNLIAGSRYA
                     TSPTTLLATLVSLDPIYFDFDMSESDFLRLSRDRARQTGILANKVELALSDETKFTRR
                     GALDFVDNVLDHSSGTIHARATVPNPEFFLTPGEFARVRLVVGAATPTLLVPDTAVLP
                     DQSQHLVMTVAADGTVAPKQVEIGAIRGGLRVIRSGLTPNDRVIIDGIPHARPGSKVT
                     LQDGAIRYAAAQGHD"
     gene            1501..2118
                     /locus_tag="VM910_18155"
     CDS             1501..2118
                     /locus_tag="VM910_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI64481.1"
                     /translation="MRVSREKFAETRERILDVAARLFREKGFDGIGLGDIMKAAGLTH
                     GGFYGHFGSKEDLQAQALSLALARSVTDWSQLIDSAAARPLSALASQYLCSDHRENVG
                     EGCALAALGVDTAREGERVRRALTAGLEPLLALLSNVMPGRSRAQRRRKAIVTIAEMV
                     GAIILARAVNDPALSAEILAATAEFIGSSDSSRTTKRRSSLPKVG"
     gene            complement(2180..2326)
                     /locus_tag="VM910_18160"
     CDS             complement(2180..2326)
                     /locus_tag="VM910_18160"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64482.1"
                     /translation="MHRLYRLRVIDALVMPAVTSTNTNAPTIMMTEKGAAMTMGAKRQ
                     RRAA"
     gene            3205..3603
                     /locus_tag="VM910_18165"
     CDS             3205..3603
                     /locus_tag="VM910_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64483.1"
                     /translation="MAAIDITAYPIHLGLGATAEIEPLFTGSMDWYSDYARRHGGDGV
                     EGRLVSMHVFKKSWDVWEMHPRGSEVVLCTSGTITLHQEGADDSRLTTILRPGQYAIN
                     DPGTWHTADVEVEASALFITAGIGTQHRPR"
     gene            complement(3638..3838)
                     /locus_tag="VM910_18170"
     CDS             complement(3638..3838)
                     /locus_tag="VM910_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64484.1"
                     /translation="MPADLADPDRAGRQRRTGVVLEERIRKDIIATHKAELEAFGGLH
                     AAPANPGAQGGRTTTAYYLNEE"
     gene            complement(4136..4546)
                     /locus_tag="VM910_18175"
     CDS             complement(4136..4546)
                     /locus_tag="VM910_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVI64485.1"
                     /translation="MSITQAHAETAIAAACRAANEIGVPMNIAVFDDAANLKAFARMD
                     GALLGSLDIALGKARTAALFGFNTEAIGEFCKPGGTSPGLENTNGGLVVFAGGIPVHG
                     ADGGVIGAIGVSGGAVAQDFDVAKAAVAAVEGQG"
     gene            complement(4629..5441)
                     /locus_tag="VM910_18180"
     CDS             complement(4629..5441)
                     /locus_tag="VM910_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64486.1"
                     /translation="MAAKKTILITGAASGFGRGAAIGMAKNGHNIISTVHVASQVTPL
                     REEVKALGLQDRIGVHRLDLTDSYDIKQALGWDFDVLWNNAGMGEPGPVWEIPIELVR
                     KNFEVNVFLPLMLTQGVVQKWVREGKKGKVVFTSSMGGLFTPANWGTYVSTKHALESV
                     AEALQQELARHGIKVQTINPGAYYTGFNETMADNPFRWLDDKANFTKQADLRKGFDDF
                     FATPEGKMDPKEMIDRMIEIVPADTGKFRNVVPKVIEDMLKEHQLQAWENQI"
     gene            complement(5586..6134)
                     /locus_tag="VM910_18185"
     CDS             complement(5586..6134)
                     /locus_tag="VM910_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI64487.1"
                     /translation="MTARSIAQESPVTAKSARRLHAHLAAAIAAMVLIAAPVTGAWAQ
                     TAQEVQMNNVSAIRKSFEAWGNGTGSPYDLLADNAEWTIVGHSAASRTYTSREDFLNN
                     VIRPFGARMATPLVPTIKNIYTDGDTVVIHFDAAGTAKDGKPYANTYAWFLKFQDGKI
                     VKATAFFDSIAFNEFWARVPSL"
     gene            6252..7163
                     /gene="gcvA"
                     /locus_tag="VM910_18190"
     CDS             6252..7163
                     /gene="gcvA"
                     /locus_tag="VM910_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVI64488.1"
                     /translation="MDRLPPLNPLRAFEAAGRWQSIRRAAEELGVTPGAVSRQVQSLE
                     AHLGTPLFRREPREMVLTAEGEEYLAVITHHFDGIREATLKLTGRRNVEILRVRAYTT
                     FAVKWLIPRLSSFHEANGDTEVRLTTSIETVDFKRENVDGAIRLGDGKWPGLEVDRLV
                     ANQLTPLCSLAFRRRANLKRPSDLVGQPLLHSLVRLDDWRIWLDAAGVAGIDPYAGPK
                     YASSTLAYQAVLQGHGIMMAQKFLFADDLRARRLVQPFSFTLDRGDFTYYFIYPRNRL
                     RNPAFRRFREWLLEQTDHKARTRVTAS"
     gene            7812..7982
                     /locus_tag="VM910_18195"
     CDS             7812..7982
                     /locus_tag="VM910_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64489.1"
                     /translation="MRPPLSARPRDRSWQFLLDRRLIAFAVCAVLFHLANAAMLPIAA
                     AAVTKRAESEAV"
BASE COUNT         1573 a         2527 c         2621 g         1666 t
ORIGIN      
        1 gaggatcgcg agcgcgccga cacccaacaa cgtcacgaag atgctgagca cagtcgcgaa
       61 gcgtgggcga tcgataaaga aatgtgtgag gcgcatgttg agcggctcgg ctctagtcgt
      121 ggccttgtgc agcggcgtag cggatcgcgc cgtcctgtag cgtgactttt gaccccggtc
      181 ttgcgtgtgg gatgccgtct atgatcactc ggtcatttgg cgtcaggccg gaccggatga
      241 cgcgcaagcc gccgcgaatg gcgccgatct cgacttgctt gggggcgacg gtaccatccg
      301 ctgcgacggt catcacgagg tgctgggact gatcgggaag aaccgcggta tccggcacca
      361 gcagggtcgg agttgcggca ccaacaacca gccttacgcg ggcgaattcg ccaggtgtca
      421 gaaagaactc agggttcgga accgtcgctc gcgcgtggat cgtgccgctc gaatggtcga
      481 gcacattgtc cacgaagtcg agcgcgcctc gccttgtgaa tttggtctcg tcgctcagcg
      541 cgagctcgac cttattggcg agaatccctg tctgccgtgc acggtcccga gaaagcctca
      601 ggaaatcgga ctcgctcatg tcgaagtcaa agtagatcgg atcgagcgag acgagcgtgg
      661 cgagcaatgt cgtcggactg gtcgcgtaac ggctgccggc aatcagattg cccaccgaaa
      721 cgaggtgcgt gccaatgcgc ccggtaaagg gggcgatgat gcggcaatgg tcaaggtcga
      781 actgagcgtc gcggatctgt gccttggcat catcaacgga agcttgcgcg gcctgcaggt
      841 cggccgtgcg ctggtcgacg gcttgcatcg tgccgaaatc ggtgcgcttc agttcctgag
      901 cgcgccaaag ctcgggcttg gcgagctcga gccgtgctga cgcactctcg gcctgcgcag
      961 tggcctgggc cagcctgatt tcgtagggac gcggatcaat ggtgaagagg aggtcgcctt
     1021 gatggactat gtccccatcc ttgaagtgta ttccggtaag cgtgccaccg acctgcgcgc
     1081 ggagctgaac ctggtcgacc gcggaaaatt gaccaaggaa gcctagtctc gaatcaagct
     1141 cgcgcacgag cggcttgctc acggtcactt gcgcaagtgg cgctcgaggc ggaggggcat
     1201 tagggctgtt tacgggatac gagtaagcaa cccatgcaat tgcgatggcc gcgatgacag
     1261 ctgcggcagc agcccaaagc ttgccgcgcg acggtcgtgc gctaggccgt tctcgctcat
     1321 cggcgtaaga cccgttgatg gtttcgctca cttgccaccc ataaatcggc gaaatcccat
     1381 ttgtgcgttt cgtcgaaagc atttgagcgc gcgactttac atgatcatcg tcatataagc
     1441 ttgccaagtt agatgtcgta catcatataa atgtcaaggg gcggatgtgt ggagctgtcc
     1501 atgagggtca gccgggagaa atttgccgag acccgcgagc gcatcctcga cgtggcggct
     1561 agactgttcc gcgagaaggg tttcgacggc attggcctcg gcgacatcat gaaggccgcg
     1621 ggactaacgc atggcggctt ctacgggcat ttcggctcga aggaggacct tcaagcgcag
     1681 gctttaagcc tcgctctggc gcggtcggtt acggattgga gccaactaat cgacagcgca
     1741 gccgctcggc ccttgtccgc gctcgccagt caatacttgt gctcagacca ccgcgagaac
     1801 gtaggcgaag gctgcgcact tgcagcgctc ggcgtcgaca cagctcgcga gggtgagcgc
     1861 gtgcgtcgtg cgctcacagc gggcctcgag ccattgctag cattactttc gaatgtcatg
     1921 ccgggacgtt ccagagctca gcgtcgccgc aaagcaatcg tgacgattgc tgagatggtc
     1981 ggcgccatca tcctggcgcg agccgttaac gatccggcgc tctcagccga gatcctcgcc
     2041 gccaccgctg aatttatcgg aagttcagac agtagccgca caaccaagag gcgatcgtcg
     2101 ctgccaaagg tgggctgaac actcgaatcg aaagccttgc aactgtgacg gcagggctcg
     2161 gctctgccgc ccaccttcgt tacgccgccc gtcgttgccg cttggcgccc attgtcatcg
     2221 ccgcgccctt ctcggtcatc atgatcgtcg gcgcattggt attggtcgag gtgaccgccg
     2281 gcatcaccaa ggcgtcgatg acccgcagcc tatagagcct atgcacacgc gttcgtcgac
     2341 gccgccaccg tcatacagga acggctggtg aggcagcagg tgccgctacg gtgcgcgcgc
     2401 agtcgagcaa ttcgtcgttc ggttagacat cgggtccccg gcagactttc ttcccggacg
     2461 accggctgca acatgggtgc ggcaaaattg gccggggcct ggtcgttacg cgagcgtcct
     2521 cgaatgtctg gttcggcaca gaccggctgt ggcggccggt ccgtaaaccc ttccgctgtt
     2581 ggacgttgac cggctgtgct gcagcggtgc cggttcttcc taatttgacc ctatgcagac
     2641 tcacttgagc gactgggacg gctacgccac tcgcgcggcg ttttaccttc ggtctcgctc
     2701 atgacggcaa cgagtaagcg cgccaaccgc gcaaggcgcg cgcctgcgtc ccggtaaaac
     2761 tcatcggtcg agggcagagg tcgtttcggc tggagtgatt cggccgcccc ggcggcagag
     2821 acggcagcca cctaagccaa tttgtactgc cgcatctcgt cggcgctcct gcgaagtctc
     2881 ggtgccgtaa tgaaaaacac gcatgccgcg gcaatgcagc gccgcattga tcctccacga
     2941 cgcggcctgc gccgccaggc atgcggcgca ctgagtcgtg ccatgcaatc ggagacggtt
     3001 cccttcaggg gcggcatact gggctcgatc gagagcgcgt tcagccaggg gcagtgtctg
     3061 acggcaggag gggtagaggt gcgtccgtga tattgccggt ggcgataaga tcggcgttat
     3121 cgaggagaag ccctgtggcg cccaatgggg ttgtcgcgcg ctccgccgac gcgaatcgcg
     3181 gcagctggtg aaggagagcg attgatggct gcaatcgaca tcacggcgta cccgattcat
     3241 ctcgggcttg gtgcgaccgc ggagatcgag ccgctgttca ccggttcgat ggactggtac
     3301 tcagactacg cgcggcgtca cgggggcgac ggtgttgaag gacgactggt gagcatgcac
     3361 gttttcaaga aatcgtggga cgtttgggag atgcatcccc gcggcagcga ggtcgtactt
     3421 tgcacctccg ggacgatcac gctccaccag gaaggcgcag atgactcgcg gctgaccacc
     3481 atcctccgcc ccggtcaata tgccatcaac gacccgggaa catggcacac tgcggacgtc
     3541 gaggtagagg catcggcgct gttcattacc gccggcatcg gcacgcagca tcgcccacgc
     3601 tgatcttccg acggctgagc atgcagacga ggaggggcta ctcctcgttc aggtagtagg
     3661 ccgtggtggt cctaccgcct tgcgcgccgg ggtttgcagg cgcagcgtgc aaaccgccaa
     3721 acgcctcaag ttcggccttg tgcgtcgcga taatgtcctt gcggatacgt tcttccagaa
     3781 ccacgccggt tcgacgctga cggccggcgc gatcgggatc agcgaggtca gcgggcaaga
     3841 catggggtcg cccctgatca tcgttagccg aagtacccga gcgcgccgcg ggcatcgatc
     3901 tgcgccccct tatggccgca gcgctggcag cggaagcgca cggcaccggg ttgtgcttgg
     3961 tccgagctga tgcaggttca ggcagcgcgg ctacacgacg atttcagagc cggaggaact
     4021 cgactacatc tgaaatcgac tagggggccg ctgcgaccga tccgaagccc cggccactag
     4081 catccggtcg ttctcgggat cccagcagtt cccgcgtttc agcgccagtt tcgggtcatc
     4141 cctggccttc gaccgccgca accgcggcct tggcgacgtc aaagtcctgc gcgaccgcgc
     4201 cgcccgacac gccaatggcg ccaatcaccc caccgtccgc cccgtggacc ggaatgccgc
     4261 cggcaaagac gaccagtccg ccgttcgtgt tctcgaggcc gggcgacgtg ccaccgggtt
     4321 tgcagaattc gccgatcgct tcggtgttga acccgaacaa tgcggctgtc ctggccttac
     4381 ccaatgcgat atcgagcgaa ccgagcagag ccccgtccat acgggcgaag gccttgagat
     4441 tggcggcgtc gtcgaaaacg gcaatgttca tcggcacacc gatttcgttg gccgcccgac
     4501 aggcggcggc gattgcggtt tcggcgtggg cttgagtgat gctcatttta tttgctcctg
     4561 ttccgtgaac gagacgccgg ccgcggcggg gagtgagcag ggggcaggcg ccgcggccgg
     4621 agagtctgtc agatctggtt ttcccaggcc tggagctggt gttccttcag catgtcctcg
     4681 atcaccttgg ggacgacgtt gcggaacttg ccggtatcgg ccgggacgat ctcgatcatc
     4741 cggtcgatca tctccttcgg gtccatcttg ccctcgggag tggcgaagaa gtcgtcgaag
     4801 cccttgcgca ggtcagcctg cttggtgaag ttcgccttgt cgtcgagcca gcggaacgga
     4861 ttgtccgcca tcgtctcgtt gaagccagtg tagtaggcgc ccgggttgat ggtctggact
     4921 ttgatgccgt ggcgcgccag ttcctgctga agcgcctctg cgaccgattc cagcgcgtgc
     4981 ttggtcgaga cataggtgcc ccagttggcc ggggtgaaca ggccacccat ggacgatgtg
     5041 aagacgacct tgcccttctt accttcgcgc acccacttct ggacaacgcc ctgcgtcagc
     5101 atcagcggca ggaaaacatt gacctcgaag ttcttgcgga cgagttcgat cgggatttcc
     5161 cagaccggac cgggctcgcc catgccggcg ttgttccaaa ggacatcgaa atcccagccc
     5221 agcgcctgct tgatgtcata agaatcagtc agatcgagcc gatggacacc gatccggtcc
     5281 tgaaggccga gtgccttgac ctcctcgcgt agcggcgtga cctgtgacgc cacatgaacg
     5341 gtcgaaatga tgttatggcc gttcttggcc atgccgattg ctgcgccgcg accaaagccc
     5401 gatgcagccc cagtgatgag gatggtcttc ttggcagcca tcatacttct cctcgattgt
     5461 ctatcggttt attttcactc ggccccgtcg agaccattgc gtcgccagca ttgagggctt
     5521 cgcgcgtgat aagcgtgccg gaacggtcgt ccgagccatt gaagtagatc tgagcaatgg
     5581 acgtcttaca gcgacgggac acgcgcccag aactcattga aggcgatgct gtcaaagaac
     5641 gccgtcgcct tcacgatctt cccgtcctga aacttcagga accatgcgta ggtgttcgca
     5701 tacggcttac cgtccttcgc cgtgccggcg gcatcgaaat gaatgacaac ggtatcgccg
     5761 tcggtataga tgttcttgat cgtcggaacg agcggggtcg ccatgcgcgc gccaaatggt
     5821 cggattacgt tattgaggaa gtcctcgcgg ctcgtatagg tgcgtgacgc ggcgctgtgg
     5881 ccgacgattg tccactctgc attgtcggcg aggagatcgt aggggctacc ggtaccattg
     5941 ccccacgcct cgaaactttt gcgtatcgcg gatacattgt tcatctgaac ctcctgtgca
     6001 gtttgcgccc aagcgccggt tactggtgcg gctatcagca ccattgcggc aatcgccgcg
     6061 gccagatggg cgtgaagtcg ccttgccgat ttcgctgtca ccgggctctc ctgcgcgatt
     6121 gatctggcag tcattgcgtc tccacgaggc cgcggtacat ctgaaggtta gcgatggcta
     6181 ttccgcggga cgaacgagaa agccgcgcgc cgtcgttgac aaaaagtcaa caattcaatc
     6241 actttggtcc gatggaccgg ttgccgccgc taaatccact tcgagccttt gaagctgccg
     6301 gccgctggca gagtatccgc cgcgccgcgg aagagcttgg tgtgacgccg ggtgcggtca
     6361 gtcgccaggt ccaaagcctc gaggcccatc ttgggacacc gctctttcgg cgtgagccac
     6421 gcgagatggt attaacggcg gagggcgaag agtacctcgc ggtaataact catcatttcg
     6481 atggaattcg cgaggcaacg ctcaagctta ccggtcggcg caacgtcgaa attcttcgcg
     6541 tccgggccta cacgaccttc gcggtgaagt ggttgatccc gcggctctcg tcatttcacg
     6601 aggcgaacgg cgacacggaa gttcgattga cgacgtcgat cgagacggtt gatttcaaac
     6661 gagagaacgt cgacggcgcg attcgcctcg gcgatggcaa atggccgggg cttgaggttg
     6721 accgcctcgt ggccaatcaa cttacgcccc tttgctcgct cgcatttcgg cgacgcgcga
     6781 acctcaaacg tcccagcgat ctcgtcggcc agccactcct gcattcgctt gtccggctcg
     6841 atgactggcg gatctggctc gatgctgccg gcgtcgccgg catcgatccg tatgccggcc
     6901 caaaatatgc gagttcgaca cttgcctatc aggcggtcct gcaaggccac ggcatcatga
     6961 tggcgcaaaa gttcctcttc gcagacgact tgcgagcgag gcgtctcgtg cagccgttct
     7021 ccttcaccct cgatcgcggc gatttcacct actactttat ctatccacgt aatcggctgc
     7081 gaaatcctgc ctttcggcga ttccgcgaat ggctgcttga gcagacggat cacaaggcca
     7141 ggacgcgggt cactgcatcc tgacttgctc gtagccggtt cgcagcggcg gccctaggga
     7201 agcggataac tttggtctcg ggcatacgca ggtagacgat gccaccaacc acgctgaccg
     7261 cggcaagata gatccaaacc gggcctcaag cttccacgcg aatgcgagtt tgtcaccact
     7321 gcaaaggtcc caacaaaacg cctgatgatg actttggtga gtccggttac cgcgacatgg
     7381 agagatggtg ccacgaaggc agctttgaat gcgaaagcgg acaccggcac ccagcccacc
     7441 tgattggaca acggaactgc cgtggtcaaa aatcgtgatg tttccgtgat ttgggcggaa
     7501 tttcgaccga cacgaaccag tgcccagttt gaccccggtt ggtccagcgg cgagtccggc
     7561 gaacttcgag cgggcgaccg gcggtatcta tcttcgcgat cccgacggca acaagatgcg
     7621 gccaatctgc tgcgccagtc atacgccgag atgctatggg gcaagcagta tttctttctt
     7681 gacaccaaca aatggcttga ggaacacggc gccgatccgc tgcccgcaag ccaacctcag
     7741 ccccgccttt ccggcagccg tttcgaggcg cattccgccc cgagcgcgcc cgcatcagcc
     7801 gggggagagc catgcggcca ccgctctcgg cgcgaccgcg cgaccgatcg tggcagtttc
     7861 tgttggaccg tcggctgatt gcctttgccg tctgcgcggt tttgttccat ctcgccaacg
     7921 cggcaatgct gccgattgcc gccgcagcgg tcacgaagcg ggcggagagc gaggcagtct
     7981 gatcatcggc ctgtatcgtt ggcccgcagc tcgtcactgc cctcctgtct ccttgggccg
     8041 ggtgggctgc agaaacttgg ggccgccggc tgatactccc catcggcttt agcgtcttgc
     8101 cgatccgcgg tttattgcgt accaaaaacg tgatttggaa tgttgtgcga ttcaggtcta
     8161 gttctcgcgg ttgggctccg cgcatttcct cagccggcaa ttcgatgagg ccgcggcgaa
     8221 actgctcctt gcgatccagg aggatcctag caacccgcag ccgccgaggg ctcagccgcc
     8281 ccttgtcctc ctcgatccgc atgtaggggc gggagaagag tgcggtggtg agcccgacga
     8341 aggcggtgag ggccacgagg aagacgttga acgggtcgat gaagaac
//
LOCUS       DATLJT010000804         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2348598,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000804 DATLJT010000000
VERSION     DATLJT010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348598"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..894
                     /locus_tag="VM910_18200"
     CDS             337..894
                     /locus_tag="VM910_18200"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HVI64490.1"
                     /translation="MTEIQALDVRIPAEIIHNWGWFLVFGIGLALLGILAIVRSVAAT
                     VVSMLFFGWLLVIAAGIEIVQAIMVGKWAGLYQHWLGAVLFGVLGALIVWRPLVTAEI
                     LTLLMGAFFLVAGLFQLITPFVVSLPDWGWHALNGVITLLLGILILVQWPVSGLWVIG
                     LFVGIELIFYGGAWIVLALNLRSLA"
     gene            982..1515
                     /locus_tag="VM910_18205"
     CDS             982..1515
                     /locus_tag="VM910_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI64491.1"
                     /translation="MTDSAKARAPILAAGGILLREGSRPRIAIVRLRRDKSWVLPKGK
                     LYPGEHAVAAAKREVMEETGHRVSVHEFLGSMSYAVDGNIKIVQFWHMRAVGGPVREL
                     TYDIKAVKWLSLKQAIDTLTRAHEKVFLANVGPIALKAAKQSARDKSTRRSVRNGGRR
                     RDRSPPGPLAEYSPAAG"
     gene            1846..>2642
                     /locus_tag="VM910_18210"
     CDS             1846..>2642
                     /locus_tag="VM910_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HVI64492.1"
                     /translation="MVFKVHVGPPQIAIHQGQTVLVSEPDGQINWPSEKGLYFLDTRI
                     VSNWAIYANGEPWELLNGGAINYHAARIFLTNKSILTEDGPIPPRTVGLTISRSIVGG
                     MHEDLDITNNNLKPVRFQLEVALRCDFADVFEVKSGKIVRRGQITTEWSERRQRLRTA
                     YSNRDFHRSLTITVSRATSKAVSANGRLSFEVALKPGQAWHACLFYMLEDRQQQLRAP
                     RDCVGTTHESRRFPTMAEWLKTVVKIKTSNEEFYRLFHQALEDMAALR"
BASE COUNT          499 a          803 c          805 g          535 t
ORIGIN      
        1 atggaggtga ggtggccttc ccacccttac cctacggctt aatcagcaag aaatagcgcc
       61 gtcgcgcctg cgcgcgcttc aacgattgcc caacaatcca acgcgtcggt gcgtgcagcg
      121 tcccgtcaaa ggccgatcct caacgtgcgc gtattaaccc gcctgtagcg tggcgccacg
      181 agcgccgacg atcagggctg aacgagcgag aatatccagc agtcgcccgg accagcttgc
      241 gttcgagtgc cgagccaaac agaatgtacc gaggaaagtg agctttccga tagggacagg
      301 gaacgtcgcg ccttttgcaa gaggagagaa cgcgccatga ccgagatcca ggctctggat
      361 gtgcgaattc ctgccgagat catccacaac tggggctggt tcctggtctt cggcatcggc
      421 ctggcgctgc tcgggattct cgcgatcgtg cgctccgtgg cggccaccgt cgtctccatg
      481 ctgtttttcg gatggctgct ggtgatcgcg gccggcattg agattgtcca agcgatcatg
      541 gtcggcaaat gggccggcct gtaccaacat tggctgggcg cggtcctgtt cggggtgctc
      601 ggagcgctga tcgtgtggcg accgctggtc accgccgaga ttttgacatt gttgatggga
      661 gcgttcttcc tggtggccgg tctattccaa ttgatcactc cgttcgtagt ttcgctgccg
      721 gactggggct ggcacgccct caatggcgtc atcacgctgt tactcggcat cctcatactg
      781 gtccaatggc cggtctccgg gctctgggtc atcggcctat tcgtcggaat cgagctgatc
      841 ttttacggcg gcgcttggat cgtgctggcg ctcaacctgc gctcgctggc ttagtcagag
      901 tcccggcata gacatcggcc aagcctctgc cgcgcgaatt ctgcgcttgc tctgcgcgcg
      961 gtcgtccggc atagctttga catgactgat tcggcgaagg cccgcgcgcc gattctggct
     1021 gcggggggaa ttttactacg ggaaggttcc agaccgcgca tcgccatcgt gcgattgcga
     1081 agggacaagt cctgggtctt gccaaaaggc aagctttatc ccggcgagca tgcggttgcc
     1141 gctgccaagc gagaggtgat ggaagagacc ggacaccgcg tttcagttca cgaatttctc
     1201 ggttcgatgt cgtacgcggt cgacggcaac atcaagatcg tccagttctg gcacatgcga
     1261 gccgtcggcg ggccggtgcg cgagttgacg tatgatatta aggctgtgaa atggctgtcg
     1321 ttgaaacagg ccatcgacac gctcacgcgc gcgcacgaaa aggtgttcct tgcgaatgtg
     1381 gggccgatcg cgctcaaggc ggccaagcaa tctgcgcgcg acaagtccac taggcggtcc
     1441 gttcgcaatg ggggcaggcg gcgggaccga agcccgccgg gtccattggc cgaatattcg
     1501 cccgcggcag gctagaacgc gcgagcatcc ggcggcgttg cgttcagctc gcccgcggcg
     1561 ctggtgagcc gcttttttcg cccttgccgc ctaaacgatc cgcgtaagac gcgcgatctc
     1621 gctcttcacc agtgcccgca gaggcttttc acccgggatt ccggcgcaaa tatccacgac
     1681 ccctctgcga gagggtcgac ctatgcggct tcgaaagaga ggcgtcctct cctctaggat
     1741 gcgggcaagg acccgtctaa gtttccgatg ttcacccgat cgatgtccga cgtgccacgt
     1801 tagctcggcg cgccggcgcc tcgcgtgcaa ccatgaggac gacacatggt attcaaggtt
     1861 cacgttggcc caccccaaat cgccatccat cagggtcaga cggtgctcgt cagcgagccg
     1921 gacgggcaga tcaactggcc gagcgagaag ggcctttatt ttctcgacac gcggattgtc
     1981 agcaactggg cgatctatgc caacggcgaa ccgtgggagc tgttgaacgg cggtgccatc
     2041 aactatcatg ccgcgcgcat cttcctcacc aataaatcca ttctcaccga agacggtccg
     2101 atccctccgc gcaccgtcgg cctcacgatc agtcgatcga tcgtcggcgg catgcacgaa
     2161 gatctcgaca tcaccaacaa caacctcaag ccggtgcgct ttcagctcga agtcgcgctg
     2221 cgatgcgact ttgccgacgt cttcgaggtg aagtcgggca agatcgtacg ccgtggccag
     2281 atcaccaccg aatggtcgga gcggcggcag cggcttcgta ccgcgtattc gaaccgggat
     2341 tttcaccgct cgctgacgat cacagtatcg cgggcaacga gcaaggccgt ttccgccaac
     2401 ggacggctca gcttcgaggt cgcgcttaag ccgggccagg cttggcatgc ctgcttgttc
     2461 tatatgctcg aggatcgcca gcaacagctt cgcgcgccgc gcgattgtgt cgggacaact
     2521 cacgagtccc gccgtttccc gacgatggcg gaatggctca agaccgtggt caagatcaaa
     2581 acaagcaacg aagaattcta ccgtctcttt caccaagcgc tggaggacat ggctgcgctg
     2641 cg
//
LOCUS       DATLJT010000805         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3268161,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000805 DATLJT010000000
VERSION     DATLJT010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3268161"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..585)
                     /locus_tag="VM910_18215"
     CDS             complement(316..585)
                     /locus_tag="VM910_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64493.1"
                     /translation="MLDRAGTVIASATQDVEPILERNQRLRGTPQTSDWGRHVATIPN
                     VILLKWLNEAHARGNTALRLFTKEFDEFAARKLADPEWKYLRTDR"
     gene            complement(607..888)
                     /locus_tag="VM910_18220"
     CDS             complement(607..888)
                     /locus_tag="VM910_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64494.1"
                     /translation="MIKDLDGDAVKDGDKDATLGRVACAALLASYADEQNLPAEDKVK
                     RFRLAEVAAKGGEQEMKIDDVALMKKLIGKAFAPLVVARAYDIIEPPSA"
     gene            complement(906..1679)
                     /locus_tag="VM910_18225"
     CDS             complement(906..1679)
                     /locus_tag="VM910_18225"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVI64495.1"
                     /translation="MAKLIARGIRAALDEMGYVLWKKEFLQYGISVYDDIARLNRAWG
                     RSVNTFFDVGANVGQTAREALRRFPSARVYAFEPHPASFLKLVAIKDDRLSAHQIALS
                     ERSGKATLYQYGEAGDGSLINSLVKNARFPIQFGHQPKKEVNVTCSTVDDFCFEHGID
                     NIDVLKLDTEGSELAVLKGAERMLASGRCSYVYTEFNELQSQPGMTGGALIPISDLLS
                     RRGLRYVATYTDFVLPNGGMHVCANALFALPSTRSLEQL"
     gene            complement(1693..2412)
                     /locus_tag="VM910_18230"
     CDS             complement(1693..2412)
                     /locus_tag="VM910_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64496.1"
                     /translation="MGRPEVRRCSRTPFADVFFDGGSIGVEIGHSGFMGSENLFLNCF
                     WLACSTAGLLTSNPNALQQTVVGGNFQGCNRAIFAGAGSVPTIQGVGFQTSAECDIYT
                     GTLSDNAMSVQGCRSESTNFINNAGGQSLHVAACSHVGSSSGCFLAQAGGFSVTSACI
                     SNLGNVVPTFWASMRIENSWFKRDDWLQLDPTHLWWKPNNARSFCLELENVSGGSTEI
                     RRQRLITLDGQTITTYNYAAS"
     gene            complement(2812..>3465)
                     /locus_tag="VM910_18235"
     CDS             complement(2812..>3465)
                     /locus_tag="VM910_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64497.1"
                     /translation="PAAIKLNLTNTASGTASRFADFQLGGTSKINLSIGGTTPTALLN
                     VAGANFGGWQVVDGSDANVANNFSYAQGYWNNNHRFFIEIGNVGTPGNTPALTIQTAA
                     GGNIILATNATTALTINGTTQAATFSASGTFGGQIATSSTSAILTGTAIPAGGTAGSG
                     YLFSSTSNYGVFFGSGAPTLSAAKGSLYLRSDGTTTNNRAYINTDGGTTWTALTTAA"
BASE COUNT          650 a         1055 c         1078 g          682 t
ORIGIN      
        1 tcctgctggc gcacgcgcag ccggcggttg gcgcaggcct cgaacagcgc gatgaattcc
       61 gggatgcgcg cactgaacag gctgtgatcg agccagttgc cgatcgcgct ctgcagctcg
      121 gcgtaggtcg tgatgctcat gcgttatctc cgcccctgct gcgccctcgt cccgctgcgc
      181 cctcgtcctg aggagcgtcg cgaaagcgac gcgtctcgaa ggacggccgc gcgtggccat
      241 gcttcgagac gcccgccata gcgcgttgaa gacgcgcgtg aacgcgctta atgcgggctc
      301 ctcagcatga ggtcatcagc gatcggtgcg caggtatttc cactccgggt cggcgagctt
      361 gcgcgcggcg aactcgtcga attccttggt gaacaagcgc agggcggtgt tgccgcgcgc
      421 gtgcgcctcg ttgagccact tgagcaggat cacgttgggg atggtcgcga catggcggcc
      481 ccaatcgctc gtctgcggcg ttccccgcag gcgctggttg cgctcgagaa tcggctcgac
      541 gtcctgcgtt gcgctcgcga tcaccgtgcc ggcgcggtcg agcaagacgg cggtacgcat
      601 tgcgggtcag gcggagggcg gctcgatgat gtcgtaggcg cgagcgacca ccaacggggc
      661 gaacgccttg ccgatgagct ttttcatgag cgccacgtcg tcgatcttca tctcctgctc
      721 gccgcccttc gctgcgacct cggcgaggcg gaaccgcttc accttgtcct cggcggggag
      781 attctgctca tcggcgtagc tcgccagcag cgccgcgcag gccacgcggc ccaacgtggc
      841 atctttatcg ccgtccttga cggcatcgcc gtcgaggtcc ttgatcacgg cggaaaagtc
      901 gattttcata gttgctccaa agatcgcgtg gacggcagcg caaataaagc attcgcgcag
      961 acgtgcatgc cgccattcgg cagaacaaaa tcagtatagg tcgccacgta ccgcaatccg
     1021 cgccgcgaaa ggagatcgga gatcggaatc aatgcccctc cggtcatccc tggctgcgat
     1081 tgtagctcat tgaactcggt gtagacgtag ctgcagcgcc cgctggcgag cattcgctcg
     1141 gcgcccttaa gcacagctag ctcggagccc tcggtgtcga gttttaggac atcgatgtta
     1201 tcaatcccat gctcgaagca gaaatcatca accgtcgagc aggtgacgtt gacctccttc
     1261 tttggctgat gcccgaactg gatcgggaag cgcgcgtttt tcactaagct gttgatcagc
     1321 gagccgtcac ccgcctcgcc gtactgatag agcgtggcct tcccactgcg ttcgctgagc
     1381 gcgatctggt gggcggacag ccggtcatcc ttgatcgcga ccaatttgag aaagctggcc
     1441 gggtgaggct cgaatgcata gacgcgagca ctcggaaagc gccgcagcgc ctcccgcgcc
     1501 gtttgcccta cgttggcgcc cacatcgaaa aaagtgttaa cagagcggcc ccaagcgcgg
     1561 ttcaggcgcg caatgtcgtc atagacggag atgccgtact ggaggaactc cttcttccag
     1621 agcacatatc ccatctcgtc gagagcggca cggatgccac gcgcgatcag ctttgccacc
     1681 atgcggccag ccctagcttg ccgcgtaatt gtatgtggtg atcgtttgcc cgtcgagcgt
     1741 tatgaggcgt tggcggcgta tctcggtcga accgccgctc acgttctcca gctcgaggca
     1801 aaatgaacgc gcattgttcg gcttccacca gagatgcgtc ggatcaagct gcagccagtc
     1861 gtcgcgcttg aaccaagaat tttcgatgcg catcgatgcc cagaacgtgg gcaccacatt
     1921 gcccaggttc gagatacaag ccgatgtcac gctgaaaccg cccgcctgcg ccagaaagca
     1981 gccactcgag ctacctacgt gactgcaggc ggctacgtgc agcgattggc cgccggcatt
     2041 gttgatgaag tttgtgctct cgcttcggca gccctgcacg ctcatggcat tgtcggacag
     2101 cgtcccggtg tagatgtcgc actcggcaga cgtctggaag cccacgcctt ggatcgtggg
     2161 caccgaaccc gcgcccgcga aaattgcccg attgcagccc tggaaatttc caccgaccac
     2221 cgtctgctgt agggcattcg gattcgacgt cagcaggccg gcggtcgaac acgcaagcca
     2281 aaagcagttg agaaacaggt tttccgaccc catgaagccg gaatgcccga tctcgacgcc
     2341 gatcgaaccg ccgtcgaaga acacgtcggc aaaaggtgtt cgactgcagc gccggacctc
     2401 cggccgaccc atcccaatcg agatcgaacg atttaccgcc cgacgcggcg gtcagccgca
     2461 tgcctccgaa gtgcgaatag ccgcagccgt tggtgacgaa gaccgaaaag ttcggcgtca
     2521 cattttcgat ttgcgtcaca aagcggccgg cgccgatgat gcgtcctccg tggagatatt
     2581 tgatggtcag cggcgcggtg accttgtagt gccccggtgg aatataaagc gccttgttca
     2641 ggcggacgtt ggccgtgcca tgcggcgcgt cggacgggcc gaagcaatga tcgattgcgg
     2701 cttgcaacgc ggcggtatcg tcggttgcaa aatcgccggc ggcaccgaag cggctgtcct
     2761 tgacgctgac cggctcgtaa tcggcaaaca agagcatcgg agcgaccatg atcacgccgc
     2821 cgtcgtcagc gccgtccagg tcgtgccgcc atcggtgttg atgtaggcgc gattgttcgt
     2881 ggtcgtgccg tcgctgcgaa gataaagcga gcccttggcg gcgctgagcg ttggcgctcc
     2941 cgaaccgaag aagacgccat agttcgatgt cgaggaaaac agatagcccg aacctgcagt
     3001 gccgccggct gggatcgcgg tccctgtcag gattgcgctc gttgagcttg tggcaatctg
     3061 gccaccaaac gtcccactag cgctaaaggt cgccgcctga gttgtgccgt tgattgttag
     3121 ggccgtcgtg gcgttcgtcg cgagaataat gttcccacca gcggcggtct gaattgtcag
     3181 ggcgggcgta ttgccgggag ttccaacgtt gccgatttcg atgaagaaac gatggttgtt
     3241 gttccaatat ccctgggcat agctgaagtt gttggcgacg ttggcatcgg agccatcgac
     3301 gacttgccag ccaccgaagt ttgcacccgc gacattcaga agagccgtcg gcgtcgtgcc
     3361 tccgatcgag agattgatct tgctcgtgcc gccgagctgg aagtcagcga agcggctcgc
     3421 cgtcccgctc gctgtattgg tgaggttgag cttgatcgcg gccgg
//
LOCUS       DATLJT010000806         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3727677,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000806 DATLJT010000000
VERSION     DATLJT010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3727677"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VM910_18240"
     CDS             <1..616
                     /locus_tag="VM910_18240"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVI64498.1"
                     /translation="GWGQGPQHTKSLHTWFAHVPGLQVVMPASPSDAKGLLLSSIMSD
                     DPTLFIEGRSLFSMQEDVPDAPYFIRLGEALVRRAGKDVTLVSMGSMVPVALRAADTL
                     SKANIDTEIVDLRCLMPLDIASIITSVKKTGRLVVAEPGWRMYGAAAEIVATVVETLG
                     EIRSRPRRVAWPQSAVPTSSRLEEQFYPTSDDIVAACRASVTES"
     gene            661..1761
                     /locus_tag="VM910_18245"
     CDS             661..1761
                     /locus_tag="VM910_18245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVI64499.1"
                     /translation="MRVPFSYLPEQFADPEPIFARLREVIAAGDFTLGKVVGEFEARF
                     ANAVGVQFAFGVGSGTDGLRLALKALGVGPGDEVITTANTFIATVGAIADTGAITKFV
                     DCADDFCMDVSLLEAAITPRTKAIIPVHMTGAMVDMPRLLEIAAARGIPVIEDACQAV
                     KSSLDKRPAGSWGLIGAFSLHPLKFINIWGDGGVMTTSDADIANRISLLRNHGLQSRD
                     EIVSLGVNSRLDTVQAVVALHVLDQADWIANQRRTNAAFYDRALAHIEQVRIPPRDPR
                     VLHSYVTYQVLVERRDALLAHCAARAIECKIHYPMPLYCQQGLRHLGYKRGDFPVTDH
                     HADTTITLPVHQYLSRDQLSFVVDSIAEFYRS"
     gene            1773..>2641
                     /locus_tag="VM910_18250"
     CDS             1773..>2641
                     /locus_tag="VM910_18250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVI64500.1"
                     /translation="MWRIPYVNPGAQFVEERAELMPRIEAILAGGMHVGGPEIEALER
                     EIADYVGTRHAVALGSGTDALILGMIAAGIGPGDEVITPPNSFVASTAAIVRAGATPV
                     FADVRRDGLIDPDAVAAAVTPRTAAVMPVHLWGGICDMEALWAIAERHGLAIVEDAAQ
                     AMGTRYRNRRAGSLGTVGCFSAHPLKIFNAVGDAGFITTDSDEIASRVRLLRNHGLVD
                     RDTVSEFGYVSRLDALQAVVLRYRLGRLDEMIERRRSNAAFYRESLNGIPIRLPEEKP
                     YEFHTFVNFVSQC"
BASE COUNT          470 a          823 c          845 g          503 t
ORIGIN      
        1 gggctggggg caagggccgc agcacaccaa gagtctccat acctggttcg cgcatgtgcc
       61 gggcctgcaa gtggtcatgc ccgcatcgcc ttccgatgcg aaggggctgc ttctctccag
      121 catcatgagc gacgatccga cgctctttat cgagggacgg tcgcttttct cgatgcagga
      181 agacgtcccc gacgcccctt atttcattcg tctcggtgaa gcgctggtgc gccgcgcagg
      241 caaggatgtc acgctcgtca gcatgggctc gatggtgccc gtcgcattgc gggccgcgga
      301 caccttgtcc aaggccaaca tcgataccga aatagttgac ctgcgctgcc tgatgccgct
      361 cgacatcgcg agcatcatca cctcggtcaa aaaaaccggc cggctcgtcg tggcggagcc
      421 cggctggcgg atgtatgggg ccgcagccga aatcgtcgcg accgtcgtcg agacgctggg
      481 cgagatccgc agccggcctc ggcgtgtcgc atggcctcaa agcgccgtgc ccaccagcag
      541 caggcttgag gagcagttct atcccacgag tgacgacatc gtcgcggcct gccgtgcaag
      601 cgtaaccgag agttgagtaa ggaacaccgc tcgttgcgtt cacattccga ggaataggcg
      661 ttgcgtgttc ctttttcata cttgcccgag cagttcgctg acccggaacc catcttcgcg
      721 cgcttgcggg aggtgatcgc ggccggtgat ttcacgctag gcaaggtggt cggcgagttc
      781 gaagcgcgat ttgcgaacgc cgtcggcgtg caattcgcat ttggggtggg ttcggggaca
      841 gacggcctgc gcctggcgtt gaaggctctc ggcgtcggac caggcgatga ggtcatcacc
      901 acggccaaca cgttcatcgc gacggtcgga gcgattgccg ataccggcgc gataacgaag
      961 ttcgtcgatt gcgcggacga tttctgcatg gacgtctcgc tccttgaagc ggccatcacc
     1021 ccgcgcacca aggcgatcat tcccgtgcac atgaccggcg ccatggtgga catgccccgc
     1081 ctgctcgaga tcgcagcggc gcgaggcatt ccggtgatcg aggatgcctg tcaggccgtc
     1141 aagtcgagcc tcgacaagcg tccggccgga tcatggggac tgatcggggc attttcactg
     1201 cacccgctca agttcatcaa tatctggggc gatggcggcg tcatgacgac gagcgacgcc
     1261 gatatcgcga accgcatcag cctgctgcgc aaccacggtc tgcaaagtcg cgacgagatc
     1321 gtgtcgctgg gcgtcaacag ccgcctcgac acggtccagg cggtcgtcgc gctgcacgtg
     1381 ctcgatcagg ccgactggat cgcaaaccaa cgccggacga atgcggcgtt ttacgatcgg
     1441 gcgttggcgc atatcgagca ggtcagaatt cccccgcgcg atccacgggt gctgcacagc
     1501 tatgtcacat atcaggtgct tgtcgaacgg cgcgacgcac tgctggcgca ttgcgccgca
     1561 cgcgccatcg aatgcaaaat ccattacccg atgccgctct actgccagca gggattgcgc
     1621 catcttggat acaaacgggg agactttccg gtgaccgatc atcatgccga tacgacgatc
     1681 acgttgccgg tgcatcagta cctgtcgcgc gatcagcttt cgtttgtcgt cgatagcatc
     1741 gcggaattct accgatcttg agggtgacag aggtgtggcg catcccctac gtgaatcccg
     1801 gagcccagtt cgtcgaagaa agggccgagt tgatgccgcg catcgaggcg atcctggcgg
     1861 gcggcatgca cgtcggcggg cccgagatcg aggcgctcga gcgcgagatc gccgactacg
     1921 tcggcacccg ccatgcggtg gcgctcggct ccggcacgga tgcgctgatt ctgggaatga
     1981 tcgccgccgg catcgggccg ggcgacgaag tcatcacgcc cccgaattcc ttcgtcgcct
     2041 cgaccgccgc gatcgtgcgc gcgggcgcca cgcctgtctt cgccgacgtc cgacgcgacg
     2101 gtctcatcga cccggacgcg gtggcggcgg ctgtgacccc gcgaacggca gcagtgatgc
     2161 cggtgcacct ctggggcggc atctgcgaca tggaagcgct gtgggcgatc gcggaacggc
     2221 acgggctcgc gatcgtcgag gatgccgcgc aggcgatggg aacgcgctat cgcaaccgtc
     2281 gcgcaggctc tttgggcacg gtcgggtgtt tctctgcgca tccgctcaag attttcaacg
     2341 cggtcggcga tgccggcttc atcaccaccg attccgatga gattgcatcg cgcgttcggc
     2401 tattgcggaa tcacggtctc gtggaccgcg ataccgtgtc cgagtttggt tatgtctcgc
     2461 ggcttgacgc gctgcaggcg gtggtcctgc gttatcgcct gggccggctc gacgagatga
     2521 tcgaacgccg acggtccaat gcggccttct accgggaatc gttgaacggc ataccgatcc
     2581 gcttgccgga agaaaagccc tacgagtttc atactttcgt gaacttcgtc tcccaatgcg
     2641 a
//
LOCUS       DATLJT010000807         7645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_738535,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000807 DATLJT010000000
VERSION     DATLJT010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738535"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VM910_18255"
     CDS             complement(<1..603)
                     /locus_tag="VM910_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64501.1"
                     /translation="MQKSSIAFWCGAAVFLIVVLVLTQTIRNEYPFFAGYVILQFVVL
                     AVAWSILGGYAGYVNFGTNAFFGVGVYTAVLLIKAFEAPLGVQIAVAAVIGSLLGFGV
                     GLLTLRMRGIFFSIATIALAIIIETSVTNWRFVGGAAGIQLQRPPVMAPFDTYVKMLF
                     FVQALLVVVAVATTRTIQNSRIGRGLQALRDDELAAECTGV"
     gene            complement(607..1482)
                     /locus_tag="VM910_18260"
     CDS             complement(607..1482)
                     /locus_tag="VM910_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64502.1"
                     /translation="MFSLDLLRDAVVLGVLLGCFYAAVSIGLSISFGLLDVPHVAHPA
                     FLVLASYGVYLCNDQYDIDPLLAGLLIAPLFFLLGLVTYRLYYETFERRGSDAAVRGI
                     AFFFGIAFIIEVLIILQFGVDQRSVTASYIGKAWRVGDLRIPIRQLVAFAVALALTVF
                     LATYLSKTFMGRAIRAVAQDEEGLRLMGANPVRIKQFAFGIATAVLGIAGALLIIVAP
                     VEPTLDRVYIGRTFCVVVMAGLGSITGTLIAAIILGVSESIVLTMFGASWAPAISFAL
                     LLGVLAVRPQGLLGR"
     gene            complement(1538..2785)
                     /locus_tag="VM910_18265"
     CDS             complement(1538..2785)
                     /locus_tag="VM910_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI64503.1"
                     /translation="MNRRDVFGWATAAVMFGLMSGASAEEPPIKIGMSMPQTGGLAGG
                     GKASLLGIEIWRDDVNAKGGLLGRKVELVVYDDKSSASETPALYAKLLDVDKVDLLFA
                     PYATVPTAPIMPLVKQRGLLLMGNFSFQVNSKIHHDMWFNNAPWGPADSWANGFLTLG
                     QKAGGKTVALLSSDQEFAQNIVTTARDVTKKIGLQVVFDQAYLPSTVEFSSIIRALNA
                     AKPDIVFVASYPPDSAAILKAVNEIGVGDNVKIFGGAMVGLQFAAVMENLGSLLNGVV
                     NYNTWLPEPSMYFDGTKEFFEKYTKRAIQAKVDPLGFYLAPYGYAMGQMIEEAINATK
                     SLDQKALAKFIRENVHKTIVGPIAYAEDGEWKESATLEAQFRNVKDKNIDQFRSAGKQ
                     VIVFPDRLKTGDLVTPFEASRGN"
     gene            complement(2863..3786)
                     /locus_tag="VM910_18270"
     CDS             complement(2863..3786)
                     /locus_tag="VM910_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HVI64504.1"
                     /translation="MRELTPETITDAVLEQMATTSDPRLKEIMAAAVKHLHDFAREVN
                     LTPGEWIKGIEFMTLVGKMCTPARQEFILLSDTLGLSALVNGLHDKTALEEGTHTSLL
                     GPFYREASPRLSAGSQIARHVKPGSECVLYGRVTDIKDKPLAAATVSVWQTSADGLYD
                     IQENPTATDYRGVFTTDAEGLYVVRTVKPRGYSIPMDGPVGNMVKAQARHGMRPAHIH
                     FLVGSPGYRELVTALYLRDDPHLADDVVFGSSDDLAVDIVPDDPQSPIKGVPSIRFDL
                     RLSREGEADRTSGRVGSDPSAILKEAAPAAH"
     gene            complement(3947..5203)
                     /locus_tag="VM910_18275"
     CDS             complement(3947..5203)
                     /locus_tag="VM910_18275"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HVI64505.1"
                     /translation="MAKAPDSTSQRTAVDLAGLPITLADVKAAADTIGGAIVATPCNE
                     SRTLGEICGCRIWLKFENLQFTSSFKERGALNCLLELSDQERRRGVIAMSAGNHAQGV
                     AYHAKRLGIPATIVMPIGTPMVKVENTRRHGADLIVTGTTVEEAGAFALVHGNARGLT
                     LIHPYDDLLVIAGQGTVALEMLQDVPDLDTLVVPIGGGGLISGMAIAAKSLKRELRVI
                     GVEAQLYPSMYNALRGTDLPMRGDTLAEGIAVKAPGRITTGVVRELVDDILLVTEDQI
                     ERAVATLITIEKTVVEGAGATGLAAVLANPALFAGRNVGLVLTGGNIDTRLIASVLTR
                     ELAREGRLTELAIDIVDRPGQLAAVAALLAEAGANIIEVSHQRTFSDLPAKATLLQLV
                     IETRDRAHLDDVLTKLSAAGLSTHRP"
     gene            complement(5203..6063)
                     /locus_tag="VM910_18280"
     CDS             complement(5203..6063)
                     /locus_tag="VM910_18280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HVI64506.1"
                     /translation="MSEPDTAAPTFCADLVRQHDFTRYAATLFVPADQRRALLALYAF
                     NVEIFRVRDQVSQPLPGEVRLQWWSDVLAGKGQGGAEGNPVAAELMLAIRHFRLPIGP
                     LSRLIDEHQFDLYNDPMPTMAALEGYVTDTSSALFALAAQIATPPSAAIEHLARHAGL
                     AQGFAHVVAALPRDASRRQLFVPTQLLAKHGSSVEAIFAGKQTSTARAAIDQLVAEAR
                     QHLDTAFSLLEAVPPETRPIFLPLAVVRRDLVRMARADTDPFEPRANSRFSTLWTLWR
                     ASKSKAFRAT"
     gene            complement(6060..6443)
                     /locus_tag="VM910_18285"
     CDS             complement(6060..6443)
                     /locus_tag="VM910_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HVI64507.1"
                     /translation="MPHQGDAPHLPRSAPIDAYGRGGFAFAGMSHRGSLLCLPDAIWA
                     WEVTRPTQIDRYSLARVFAAANGIDTLIVGTGTEVWLPPQELRAALRAVRIVLDAMQT
                     GPAIRTYNIMIGERRRVAAALIAVP"
     gene            complement(6492..7502)
                     /gene="secF"
                     /locus_tag="VM910_18290"
     CDS             complement(6492..7502)
                     /gene="secF"
                     /locus_tag="VM910_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVI64508.1"
                     /translation="MTHIVLISLGVLIAVLTVVSALGILPSLRIVPDDTHFDFTQFRR
                     ISFPISATLSIVAITLFFTHGLNFGIDFKGGTLMEVRAKSGTADIGSMRGSLSTRGLG
                     DIQLQQFGGPADVLIRVAEQPGGDAAQQEAVNKVRGALGDAVEYRRVEVVGPRVSGEL
                     LAYGMVGLMLAIFAILIYLWFRFEWQFALGAMIANVHDIVLTIGFMSITQVDFDLTSI
                     AALLTILGYSLNDTVVIYDRIREMLRRYKKMPMPQLLNESINSTLSRSIITHLTVSLA
                     LLALLLFGGHAIHSFTAVMMFGVVLVGTYTSIFIAAPVLIYLGVGTHRADAPEVPPKK
                     RG"
BASE COUNT         1558 a         2373 c         2354 g         1360 t
ORIGIN      
        1 cacgcccgta cactcggccg cgagttcgtc gtcgcggagc gcctgcagcc cgcggccaat
       61 ccgggagttc tggatggtcc gtgtggttgc gacggccacg accaccaaca gcgcctgcac
      121 gaagaacagc attttgacat aggtgtcgaa cggcgccatc accggcggtc gctggagctg
      181 aatgccggcg gcgccgccga cgaacctcca gttcgtcacc gacgtctcaa tgatgatcgc
      241 cagcgcaata gtggcaatgg agaaaaaaat gccgcgcatg cgcagggtca gcaggccgac
      301 gccgaatccg agcaagctgc cgatcactgc ggccactgcg atctggacgc cgagcggcgc
      361 ctcgaaggcc ttgatcaaca gcacggccgt atagacgccg acgccgaaaa atgcgttggt
      421 tccgaaattg acgtagccgg cgtagccgcc caaaatgctc caggcgaccg caagcaccac
      481 gaattgcagg atcacgtaac cggcaaaaaa cggatattca ttacggatcg tctgcgtcag
      541 caccagcacg acgatcagaa aaaccgctgc tccacaccag aatgcgatcg agctcttctg
      601 catgggtcac ctgccgagca ggccttgcgg ccggacggca agcactccca ggagcaacgc
      661 aaacgaaatc gccggtgccc aggaggcgcc gaacattgtc aggacgatcg attcggaaac
      721 accaagtatg atggcggcaa tcaacgtgcc tgtgatactg ccgagcccgg ccatgacgac
      781 gacgcaaaag gttcgcccga tataaacgcg atcgagtgtg ggctcgacgg gagccacgat
      841 gatcagaagc gcgccggcga tgcccaggac cgcagttgcg ataccgaacg cgaattgctt
      901 gatccggacc gggtttgcac ccatcaggcg caatccctcc tcgtcctgcg ccactgcgcg
      961 gatcgcacgc cccatgaagg tctttgacag gtacgtagcc aggaaaaccg tcagcgcgag
     1021 tgcgaccgcg aaggcaacca attgccggat cgggatccgc aagtcgccga cccgccaggc
     1081 cttgccgata taggatgcgg tcaccgagcg ctgatcgacc ccgaactgca ggatgatcag
     1141 gacttcgatg atgaaggcaa taccgaagaa gaaggcaatg ccgcgcacgg cggcatcgct
     1201 gccacgccgc tcgaaggtct cgtagtaaag ccggtaggtg accagtccga gcagaaagaa
     1261 cagcggcgcg atcaggagcc cggccagcaa tggatcgatg tcatactgat cgttgcatag
     1321 gtagacgccg taggaggcca gcaccaggaa ggccgggtga gccacgtggg gcacgtcgag
     1381 caagccgaac gagatcgaga gcccaatgct gacggctgca tagaagcagc cgagcagcac
     1441 gccgagcacc actgcatcgc gcagcaagtc caacgaaaac acctgaaccg ccctcctcca
     1501 ccgccctcaa gccggccccc gaaaggtagc gggccatcta attgccgcgc gacgcctcga
     1561 acggcgtaac gaggtcaccg gtcttcaatc ggtccggaaa cacaatcact tgcttgcccg
     1621 cagaacggaa ctggtcgatg ttcttgtcct tgacgttgcg gaattgcgcc tcgagtgtcg
     1681 cgctctcctt ccattcaccg tcttctgcgt aggcaatcgg cccgacaatg gtcttgtgta
     1741 cgttttcgcg aatgaatttt gcgagcgcct tctggtcgag cgatttcgtc gcgttgatgg
     1801 cctcctcgat catctgaccc atggcgtagc cgtaaggcgc aaggtagaag ccgagcggat
     1861 cgaccttagc ctggatggca cgcttggtgt atttttcgaa gaactccttg gtgccgtcga
     1921 aatacatgct cggttccggc agccacgtgt tgtaattgac gacgccgttg agcagcgatc
     1981 ccagattctc catcacggcg gcgaattgca ggccgaccat ggcgccgccg aagatcttga
     2041 cgttgtcgcc gaccccaatc tcgttgacgg ccttcaagat cgccgcagag tccggtggat
     2101 aggatgcaac gaacacgatg tcgggttttg cggcattgag tgcgcgaatg atgctggaga
     2161 actcgaccgt gctcggcaga taggcctggt caaacacgac ctgaagaccg atcttcttcg
     2221 tgacgtcgcg cgctgtcgtt acgatattct gggcaaactc ctggtcggag gagagaagcg
     2281 caacggtctt gccgccagcc ttctggccga gcgtcaggaa gccgttcgcc cagctatcag
     2341 ccggtcccca gggcgcattg ttgaaccaca tatcgtgatg aatcttgctg ttcacctgga
     2401 aggagaaatt gcccatcagc aacagaccgc gctgcttgac caacggcata atgggcgcgg
     2461 tcggcaccgt cgcatagggt gcaaacaata gatcgacctt gtcgacgtcc aggagcttcg
     2521 cataaagcgc cggcgtctcc gaagcgcttg atttgtcgtc atagaccacc agttcgactt
     2581 tgcggccaag caagccaccc ttggcgttga cgtcgtcgcg ccaaatctcg atccccagga
     2641 gcgaggcctt gcccccgccg gcgagcccac cggtttgtgg catggacatg ccgattttga
     2701 tcgggggttc ttccgcgctt gcgccggaca tcagtccgaa catgacagcc gccgtcgccc
     2761 agccgaaaac gtctctacga ttcattgatt gtccccaata ggcattgttg cgttgttggc
     2821 actctagtta cgtgaaacct tgattgatgt tcaacacccg cgctagtgcg ctgcaggcgc
     2881 cgcctctttc aggatcgccg aaggatcgga gccgacgcgt ccgctcgtcc tgtcggcttc
     2941 gccttcgcgc gacagccgca ggtcgaaacg gatgcttggc actcccttaa tgggagactg
     3001 gggatcatcc ggcacgatat cgaccgcaag atcatcggag gagccgaaca cgacgtcgtc
     3061 ggcaagatgc ggatcatccc gcaaatacag tgccgtcacc aactcgcgat agccgggcga
     3121 gccgactaga aagtgaatat gggccgggcg catgccatgc cgcgcctgtg ctttcaccat
     3181 gttgccgaca ggcccgtcca tcggaatcga atatccgcgg ggcttgacgg tccgcaccac
     3241 atagagccct tccgcatcgg tggtgaagac accgcgataa tcggtggcgg tcgggttctc
     3301 ctggatgtca tagagaccgt cggcgctggt ttgccagacc gagacggtcg cggcggcgag
     3361 aggcttgtcc ttgatgtcgg tgacgcgacc gtaaagcacg cattcgctgc cgggcttgac
     3421 gtgcctggca atctgactgc cggccgaaag cctgggcgac gcttcgcgat agaatgggcc
     3481 gagcaggctc gtatgcgtgc cttcctcgag ggcggtcttg tcgtgcaagc cgtttacgag
     3541 ggccgacaga ccgagcgtgt ccgaaagcag gatgaactcc tggcgcgccg gcgtacacat
     3601 cttgccgacc agggtcatga attcgatgcc cttgatccat tctcccggag tcaaattcac
     3661 ttctcgcgcg aagtcgtgaa ggtgcttgac cgcggccgcc atgatctcct taaggcgcgg
     3721 gtcggacgtg gtcgccatct gctcgagcac ggcgtcggtg atcgtctccg gcgttaactc
     3781 ccgcatggtc cgtctccctg atctgccggc ttgtgcggca gcttcgccaa gcctaccgct
     3841 ctctacccga gaacgtccag tgtgctgaag gatttacagt ggatttgaac gaacgtcctg
     3901 ccgcatactt tggtcgcact gccgcaatcg gtaccggcac gcaaattcag ggccggtgtg
     3961 tgctcaatcc cgcggcactc aattttgtca gcacgtcgtc cagatgcgca cgatcccgtg
     4021 tttcgatcac caactgcagc agggtcgcct tggccggcag atcggagaag gttcgttgat
     4081 gcgacacctc gatgatattg gccccggctt ccgccagcag ggcagcgacc gccgccaact
     4141 ggccgggcct gtcgacgatg tcgatggcga gttcggtcag gcgaccctct cgcgcgagtt
     4201 cacgggtcaa gacggaggca atcagccgcg tatcgatgtt gccgccggtc agaacgaggc
     4261 cgacgttgcg tcccgcaaac agcgctggat tggcgagaac cgccgctagc ccggtcgcgc
     4321 ctgctccttc gacgaccgtc ttttcaatgg tgatcagcgt cgccactgcg cgttcgatct
     4381 gatcctccgt cacgagaaga atgtcgtcga cgagttcgcg aacgacgccg gtcgtgatcc
     4441 gtcccggcgc cttgactgcg ataccttcgg ccagcgtatc gccgcgcatt ggcagatccg
     4501 tacctcgaag ggcgttatac atcgacggat agagctgggc ctcgacgccg atgacccgca
     4561 gctcccgttt caatgacttt gccgcgatcg ccatgcctga aatgagcccg ccgccgccga
     4621 tcggaacgac gagcgtatcg aggtccggca cgtcctgcag catttccagc gcgacagtgc
     4681 cctgccccgc aatgacgagc aggtcgtcat agggatggat cagcgtcaag ccacgcgcgt
     4741 tgccgtggac gagtgcgaag gcgcccgctt cctcaaccgt tgtgccggtc acaatgaggt
     4801 cggcgccgtg ccgacgggtg ttttccacct tcaccatcgg ggtgccgatc ggcatgacaa
     4861 tggtcgcggg aataccaagg cgctttgcgt gatacgctac gccctgcgcg tgatttccag
     4921 ccgacatcgc gatcacgccg cgccgccgct cctgatccga gagctccagg aggcaattta
     4981 gagcgccgcg ttccttgaag ctggacgtaa actgcaggtt ctcgaatttg agccagatcc
     5041 ggcacccgca aatctcgccg agcgtcctgc tttcattgca aggggtcgcg acgatcgcac
     5101 cgccgatcgt gtctgccgca gccttcacgt ccgccagcgt gatcggcagg ccggcaagat
     5161 cgaccgcggt tcgctgcgaa gtgctgtcgg gagcctttgc catcacgtcg ccctgaatgc
     5221 ttttgacttc gacgcccgcc acagcgtcca cagggtagaa aatcgcgaat tggcccgcgg
     5281 ctcaaaaggg tcggtatcgg cgcgcgccat ccgaacgaga tcgcggcgga cgacggcaag
     5341 cggcaggaag atcggccgcg tctccggcgg aacggcctca agcagggaga atgcggtgtc
     5401 gaggtgctgc cgagcctccg cgacgagctg gtcaatcgca gcgcgggccg tcgacgtctg
     5461 cttgcctgca aagatcgcct ccacgctgct gccgtgtttc gccaacagtt gcgtcggtac
     5521 gaataattgc cggcgcgacg cgtcgcgcgg caacgcggcg accacgtgag caaagccctg
     5581 cgcgagcccg gcatgccgcg ccaggtgctc gatcgcggcc gacggcggcg tcgcaatttg
     5641 cgcggccagc gcaaacagtg ccgacgacgt gtcggtgaca taaccttcga gtgccgccat
     5701 ggtaggcatg gggtcattgt agagatcgaa ctggtgctcg tcgatcagac gcgacaacgg
     5761 gccgattgga aggcggaagt ggcggatcgc aagcattagc tcggccgcga cgggattgcc
     5821 ctcggccccg ccctggccct tccccgccag tacgtcgctc caccactgca gccggacttc
     5881 gccgggcagc ggctggctga cctggtcgcg tacccggaaa atctccacgt tgaacgcgta
     5941 tagcgcgagc agcgcgcgac gctggtccgc tggtacgaac aacgtcgccg cataacgcgt
     6001 aaaatcgtgc tgacgcacga gatcggcgca gaatgtgggc gccgcggtgt cgggttcgct
     6061 catggcaccg caatcaaggc ggcggcgacg cgccgccgct cgccgatcat gatgttgtat
     6121 gtacggatcg cgggtcccgt ctgcatggcg tccagcacga tcctgacggc acgcagggcc
     6181 gcccgaagct cctgcggcgg caaccagact tcggttccgg tcccaacgat cagggtatcg
     6241 atgccgttgg cggcggcgaa cacgcgcgcg agcgaatagc ggtcgatttg tgtcggtcgc
     6301 gtgacctccc aggcccagat tgcatccggc aggcaaagca aagagccgcg atgtgacatg
     6361 ccggcaaagg cgaagccgcc cctgccatag gcgtcgatcg gcgccgacct cggaagatgc
     6421 ggagcgtcac cttgatgggg catgatcttg tccgatttcg acttcccgct aacgcggcac
     6481 gcgggtcgcg atcaacctcg cttcttcggc gggacttccg gtgcgtccgc ccgatgggtg
     6541 ccgacgccga gataaatcag aacgggcgcc gcgatgaaga tcgaggtgta agtgccgacc
     6601 agaaccacgc cgaacatcat gaccgccgtg aagctgtgaa tggcatggcc gccgaacaaa
     6661 agcagcgcga gcagggccag cgacaccgtc aagtgggtga tgatcgaacg tgacagggtg
     6721 gaattgatcg attcgttcag gagctgcggc atgggcattt tcttgtatcg ccgcaacatt
     6781 tccctgatgc ggtcgtagat cacgactgtg tcgttcagcg aatagccgag gatggtgagc
     6841 agcgccgcaa tgctggtgag atcgaaatcc acctgggtaa tcgacatgaa gccgatggta
     6901 agcacgatgt cgtgcacgtt ggcgatcatg gcgccgagcg cgaactgcca ttcgaagcgg
     6961 aaccagaggt agatcaggat cgcgaagatc gcgagcatca ggcccaccat gccgtaggcc
     7021 aaaagctcgc cggaaacgcg ggggccgacc acctcgacgc ggcggtactc cacggcatcg
     7081 cccagcgcgc cgcgaacctt gttcaccgcc tcctgctgcg ctgcatcgcc accgggttgc
     7141 tcggcgaccc gaatcaagac gtcggccggt ccgccgaact gctgcaactg gatgtcgcca
     7201 aggccccgcg tgctgaggct cccgcgcatc gagccgatgt cggcagtacc ggacttggcc
     7261 cgcacctcca tgagagtgcc gcccttgaaa tcgatgccaa aattcaggcc gtgggtgaag
     7321 aacagcgtga tcgctacgat cgacagcgtc gccgaaatcg gaaaggagat gcgacggaat
     7381 tgcgtgaagt cgaaatgggt gtcgtcgggg acgatgcgca gcgacggcaa gatcccgagc
     7441 gcgctgacca cggtcagcac ggcaatcagc acaccgagag aaatgagaac gatatgtgtc
     7501 acggcaggcc gaagctccct agatcggcac gctttgcggc cgtttccacc gcacccacca
     7561 cgccacgatg aggcgggtaa gcgtgaatgc ggtgaaaacc gtggtgatga tgccgatgcc
     7621 gagcgtcacg gcgaagccgc gcacc
//
LOCUS       DATLJT010000808         4355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1430097,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000808 DATLJT010000000
VERSION     DATLJT010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430097"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VM910_18295"
     CDS             <1..550
                     /locus_tag="VM910_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64509.1"
                     /translation="AADGYANRRVAVFDSETGNYKRHWGAYGKSPSDEKTPPYDPAKP
                     PSQQFGNPVHCIRIDKDDLVYVCDRSNNRLQLFRKDGTFVAEHVYEKATRGTGSVYDL
                     VFSPDQDQKFIYMIDGMNGEVRIVDRASKETLGRFGRPGRQVGQFTALHNIAVDRQGN
                     IFTSEVNTGQRVQKFRRIDIQN"
     gene            725..1717
                     /locus_tag="VM910_18300"
     CDS             725..1717
                     /locus_tag="VM910_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HVI64510.1"
                     /translation="MSLPDFSMRQLLEAGVHFGHQAHRWNPKMAEYIFGARNNIHIID
                     LAQSVPMLHRALQAVSDTVANGGRILFVGTKRQAQDAIAEAAKKSAQYYVNSRWLGGT
                     LTNWKTISQSISRLRKLDEMLSSGASQGYTKKERLTLQRERDKLDRSLGGIKDMGGLP
                     DLLFVIDTNKEDIAIKEARRLGLPVAAVVDTNCDPDGITYVVPGNDDASRAITLYCDL
                     VAKAAIDGISRAQGELGVDTGAAAEPIAEELPAAAEVPSYEVLSGPRGVADDLKKLHH
                     VSPAIEKKLNDLGIFHYWQIAGLGPDAAHTIGEEVGLPGRVEGWITQSKELTSE"
     gene            1876..2799
                     /gene="tsf"
                     /locus_tag="VM910_18305"
     CDS             1876..2799
                     /gene="tsf"
                     /locus_tag="VM910_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HVI64511.1"
                     /translation="MANITAQMVKELRERTGAGMMDCKAALQETGGKMEQAVDLLRKR
                     GLAKAAKKAGRIAAEGLIGLAVEGSQGVVVEVNSETDFVARNELFQGLVKMIANVALT
                     VGADVEKIKEAKVGNITVAEGIADTIAKIGENMSLRRASSLSAANGVIGSYVHNSVDV
                     GLGKIGVIVALESTEKGDELKRFGRMLAMHVAASNPQAIDPSGLDPDVVRREKEVLAD
                     KYKAQGKPANVIDKIVESGLKTFYKEVCLLDQAFIHDPDKSVSQAIKDVEGKVGAPIK
                     VTGFVRYVLGEGIDRPDDDFGGEVAALIKPN"
     gene            2883..3599
                     /gene="pyrH"
                     /locus_tag="VM910_18310"
     CDS             2883..3599
                     /gene="pyrH"
                     /locus_tag="VM910_18310"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVI64512.1"
                     /translation="MTKPVYRRVVVKVSGEALMGPDSFGIHQPTLERIAGDLIASQRL
                     GVAIAVVVGGGNIFRGVNVSKKGIARATGDMMGMLATVMNALALEGAIEKAGLAARTM
                     SALTMPEVCEAYERRRALRHLEQGRMVLLAGGTGNPFFTTDTTAVLRAAEIGAEAVLK
                     ATDVDGIYSADPKKDQNAKRYDRLTHQEAIERNLKVMDTTAFALARENRMPIIVFSIR
                     EKEAIAAVLRGKGRATVVGT"
     gene            3673..4236
                     /gene="frr"
                     /locus_tag="VM910_18315"
     CDS             3673..4236
                     /gene="frr"
                     /locus_tag="VM910_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVI64513.1"
                     /translation="MANPTHDINELKRRMQGAIQLLKQELGGLRTGRASASLLEPVQV
                     EAYGTHMPLNQVATVSVPEPRLISVQVWDKSMVHAVEKAIAASNLGLTPSTEGQVLRL
                     RIPELNEERRKDLVKVAHKYAEAARVAVRHVRRDGLDVLKRLEKDHKISEDDGKRSSE
                     QVQKATDEAITEVDKMLATKEREILTV"
BASE COUNT          898 a         1343 c         1402 g          712 t
ORIGIN      
        1 tgccgcggac ggctacgcca accggcgcgt cgcggtgttc gattccgaaa ccggcaacta
       61 caagcgccac tggggcgcct acgggaaatc gccgagcgac gaaaagacgc cgccctacga
      121 tcccgcaaag ccgccctcgc aacagttcgg caacccggtg cattgcatcc gcatcgacaa
      181 ggatgatctc gtctacgtct gcgaccgcag caacaaccgc ctgcagctgt tccgtaagga
      241 tggcactttt gtcgccgagc acgtctacga gaaggcgacg cgcggcacgg gctcggtcta
      301 cgacctcgtg ttctcgcccg accaggacca aaaattcatc tacatgatcg atggcatgaa
      361 cggcgaggtg cgcatcgtcg accgcgcctc gaaggagacg ctcggccgct tcggccggcc
      421 agggcgacag gtcggccagt tcacggcgct gcataacatc gccgtcgacc gccaaggaaa
      481 catcttcacg tccgaggtca ataccggcca gcgcgtgcag aagtttcgcc ggatcgacat
      541 ccagaactag gggcaaagcc cggcggcggc gtcctcgctg gatccttgcg tcggccgatt
      601 catgcctata taagccccgt catctcacac gcggatcttg gcttcacggc cgtccggtgg
      661 ctcgttccga ccgcaaggaa agaggagctc acgggtccgc ggcggctcaa ccggagaaca
      721 agatatgtcg cttccggact tctccatgcg tcagctgttg gaggctggcg tccacttcgg
      781 ccatcaggcc caccgctgga atccgaagat ggcggaatac atcttcggcg cccgcaacaa
      841 tatccacatc atcgacctcg cccaatccgt gccaatgctg caccgcgcgt tgcaggcggt
      901 gagcgatacg gtcgccaatg gcgggcgcat cctgttcgtc ggcaccaaac gccaggcgca
      961 ggatgccatc gctgaggcgg ccaagaagtc ggcgcagtac tacgtcaatt cgcgctggct
     1021 cggcggtacc ctcaccaact ggaagaccat ctcgcaatcg atctcgcggc tgcgcaagct
     1081 cgacgagatg ctctcctcgg gagcgtccca gggctacacc aagaaggagc gcctcacgct
     1141 ccagcgcgag cgcgacaagc tcgaccgctc gctcggcggc atcaaggata tgggcgggct
     1201 tcccgacctc ctgttcgtca tcgataccaa caaggaggat atcgccatca aggaggcgcg
     1261 ccgtctcggc ctgccggtcg ccgccgtcgt cgataccaat tgcgatcccg acggcatcac
     1321 ctatgtggtg cccggcaatg acgacgccag ccgcgccatc acgctctatt gcgatctcgt
     1381 ggcgaaggcg gccatcgacg gcatttcgcg agcccagggc gaactcgggg tcgataccgg
     1441 ggcggcggcc gaaccgatcg cggaagagtt gccggcggcc gccgaagtgc cgagctacga
     1501 ggtactttct ggtccgcgtg gcgtcgccga cgatctcaag aagctccacc atgtctcgcc
     1561 ggccatcgag aagaagctca acgatctcgg gatcttccac tattggcaaa tcgccgggct
     1621 cgggcccgac gccgcccaca cgatcggtga ggaggttgga cttcccggcc gggtggaagg
     1681 ctggattacg cagtcgaaag agttgacctc agagtagtac cgccgtcagg ccggcttggt
     1741 ttgggcatcc atggccccgc cacgctgaac gaaagacgtg ggacgccacc ggaccttggc
     1801 gcgtgcgagt tccccacgtg aagtgccgct tatggcgaca agcccggccg ggacgaacat
     1861 aggaaaatca cacccatggc caacatcacg gcgcaaatgg tgaaggagct gcgcgaacgc
     1921 accggcgcag gcatgatgga ctgcaaggcg gcgttgcagg aaaccggcgg caagatggag
     1981 caggccgtcg atttgttgcg caagcgggga ctcgccaagg ccgccaagaa ggccggccgc
     2041 atcgccgccg aaggcctcat cggactcgcc gtggagggct cacagggcgt cgtggtcgag
     2101 gtcaattccg agaccgactt cgtcgcccgc aatgagttgt tccagggcct ggtgaaaatg
     2161 attgcgaatg tcgcgctcac cgtcggcgct gacgtcgaga agatcaagga ggcgaaggtg
     2221 ggcaatatca ccgtggccga gggcattgcc gacaccatcg ccaagatcgg tgagaacatg
     2281 tcgctgcgac gggcgtcgtc cttgtcggcc gcaaacggcg tcatcggcag ctacgtgcac
     2341 aactccgtcg acgtaggcct cggcaagatt ggggtcatcg tggcgctgga atcgaccgaa
     2401 aagggcgacg agctcaagcg cttcggccgt atgcttgcga tgcacgttgc ggcgtccaat
     2461 ccgcaggcga tcgatccctc cggcctcgac ccggacgtgg tccggcggga gaaggaggtg
     2521 cttgccgaca agtataaggc gcagggaaaa cccgcgaatg tgatcgacaa gatcgtcgaa
     2581 tcagggctca agacctttta caaggaggtg tgcctgcttg atcaggcctt catccatgat
     2641 ccggacaaga gtgtttcgca ggcgatcaag gacgtggagg gcaaggtcgg cgctccgatc
     2701 aaggttacgg ggtttgtgcg ctatgtgctc ggcgaaggca tcgaccggcc ggatgacgat
     2761 ttcggcggcg aggtcgcggc cttgatcaag ccgaattgag tcgttcaagc ggcattgagc
     2821 gtcccaccca tgcgatagcc gtgtcgagcc actcgcgccg gcgggaaagc gcaggagtta
     2881 ccatgacaaa acccgtctac cggcgcgtcg tggtgaaggt ttccggcgag gccctcatgg
     2941 gccccgacag cttcggcatc catcagccga ctttggaacg gatcgcgggc gatcttatcg
     3001 cctcccagcg gctgggcgtg gcaattgccg ttgtggtcgg gggcggcaat atctttcgcg
     3061 gtgtgaacgt gtccaaaaaa ggcattgcgc gcgcgaccgg cgacatgatg ggcatgctcg
     3121 ccaccgtcat gaacgcgctg gcgctcgagg gcgcgatcga aaaggcgggg ctggcggctc
     3181 gtaccatgtc ggcgctcacc atgcccgaag tctgcgaggc ttatgagcgg cgtcgcgcgt
     3241 tgcgccatct cgagcagggg cggatggtcc tgctcgcggg ggggaccggc aatccgttct
     3301 tcaccacgga tacgactgcg gtgttgcgag cggccgagat cggcgccgag gcggtactca
     3361 aggcgaccga cgtcgacgga atctacagcg ccgatccgaa gaaggaccag aacgccaaac
     3421 gctatgaccg cttgacccac caggaagcaa tcgagcgcaa cctgaaagtg atggatacca
     3481 ctgccttcgc gcttgcccgg gaaaatcgta tgcctatcat cgtgttctcg atccgcgaaa
     3541 aggaggcgat cgcggcggtg ctgcgcggga aagggcgcgc caccgtcgtc ggcacttgac
     3601 gcgctgtcgc ccatcgcgtc atgcgtaacg caaaacgacg cgggatcgac taatttgttc
     3661 gaggggatcg ccatggcaaa cccgacgcat gacatcaacg aactcaagcg ccgcatgcaa
     3721 ggtgcaatcc agctgttgaa gcaggagttg ggcgggctgc gcaccggccg cgcctcagcg
     3781 agcctgctcg agccggtgca ggtcgaagct tacggcaccc acatgccgct caatcaagtg
     3841 gccaccgtga gcgtacccga gccccggctc atttccgtgc aagtgtggga caaatcgatg
     3901 gttcacgccg tcgaaaaggc gatcgccgcg tccaatctcg gtcttactcc ttcaaccgaa
     3961 ggccaagtgc tgcggctgcg catccctgaa ctcaacgagg agcggcgtaa ggacttggtc
     4021 aaagttgcgc acaaatacgc cgaggcggcg cgggtcgccg tgcggcacgt gcggcgtgac
     4081 ggccttgacg tgctcaaaag gctcgaaaag gatcacaaga tcagcgaaga cgacggcaag
     4141 cgctcgtccg agcaagtgca aaaggcgacg gacgaagcaa tcaccgaggt cgacaagatg
     4201 ttggcgacca aggaaaggga aatcctcacg gtgtgaggtt ggagcgcgat ggcgaagacc
     4261 gggccgctgc ccactgccga gatcggcgat ttcgccgtgc cgcggcacgt tgccatcatc
     4321 atggacggca acggacgctg ggcggcggcg cgcgg
//
LOCUS       DATLJT010000809         3838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2580017,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000809 DATLJT010000000
VERSION     DATLJT010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580017"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VM910_18320"
     CDS             complement(<1..1573)
                     /locus_tag="VM910_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64514.1"
                     /translation="MKAAGPFRYVAAASTHAIVLAYAIGNVPARTWHADSAILDWDHA
                     PDELRNAFEHGATFAAWNASFDTAVWNYATLGFPFLTPERIIDPMVQAGVSNLPTDLE
                     SASRYLGGAGKQKDGKRLIKLFCIEGANPHEYPAEWERFLAYARQDIEAMREVYRRTR
                     PLPHEEWQQYWAFEHVNRRGVALNMPYVRRAAALAAEDAVAIGGRLVVLTDGAVTRVT
                     QAKKIATWLHDNLADAAMREILMVGVPADDGDDDDADNGDEDEPQELSLTRDRVARVL
                     AMLDIKRANGGLSPNEMKAHEAAALHLFGAGASPKKFARLEAQQVDGVLRGQYRFAGA
                     GQTGRLTSHGAQIQNLTQDLLGEDGAAESPLVEAIAKGCDYATLVAADPVDMPAARKL
                     ALIVRPALVASPRTLLVWSDWSAIEARITPWLAASEGAERVLDIFRANDRDPSLPDSY
                     VIAAADIFHKDVRTITKPERQIGKVVVLACGFGGGVGALQAMAFSYRIHLEDAEARRI
                     VDAWRAANPWAREFWG"
     gene            complement(1711..2454)
                     /locus_tag="VM910_18325"
     CDS             complement(1711..2454)
                     /locus_tag="VM910_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64515.1"
                     /translation="MSNDHDHDRDRDHANDNGEHERLGDKLAPAPAAGGALTSLTALQ
                     TVLNRVDTASIAGRSGLPMMQFKREGDGTWSFGQKRIVPEKGSRWAVNPTTFKWGFIC
                     FDEANKPTERLVSVSQPKPGITTLPDTGFPWQEQWAVNMKCLDGTDAGVEVTFKASTV
                     GGTQAVAGLVELVRDRLNSGQHYGQIAPIVFLEKDSYPHSQYGKVWIPVMTVVDWMPL
                     DGPAPTPAPAPAPGSASPPVEQPRRRRVS"
     gene            complement(2510..>3838)
                     /locus_tag="VM910_18330"
     CDS             complement(2510..>3838)
                     /locus_tag="VM910_18330"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI64516.1"
                     /translation="ARCSCLEFTGHAARSYHRGGRGCTMNVELPREPAHSPFGGSVAA
                     RILRCPASVGLVGKVPPHLRKVSAYADRGTALHAAITCLLAENTHALVESLAGKTFGS
                     YTITHDDVETALWPAYAYVETLLDAPGAEYYLERRVIFPAIVDTFGTCDLLIRISRKI
                     YVIDFKFGVGVRVLALSPEGDEDVINAQLLFYAAAARHSLPEFFAGVDEIALTILQPM
                     SIELDAEMVSSVAVTHAELDAFVAAYRSACEEALSSAPRLQLGTHCRFCPARPICPVH
                     TGPLLDLAHFAMSPSSTTKEAYLQMLAYGLELVDAIKDLRTALHDQAKRALEEGDHVP
                     GYALSAGRAERHWHNEDAAIGALIRLGLARGDVIAETIRSPKQVELRAKARGLKVPAE
                     LIVSRRSGVSLVRSENAHVPVPGRVELARTFSEALAAFQEGGNHEPRSQS"
BASE COUNT          674 a         1176 c         1241 g          747 t
ORIGIN      
        1 ccccccagaa ctcccgcgcc caaggatttg cagcccgcca ggcatcaacg atgcgacgcg
       61 cttcggcgtc ctcaagatgg atgcgatagc tgaatgccat cgcctggaga gctccgaccc
      121 cgccgccaaa accgcaggcg aggaccacca ccttgccgat ctgtcgctct ggcttcgtta
      181 tcgtgcgcac gtctttgtgg aagatgtcgg ccgcggcaat gacgtaactg tcgggcagcg
      241 atgggtcgcg atcgttagcg cggaagatgt cgagaactct ttcggcgcct tctgatgcgg
      301 cgagccaagg cgtgatccgc gcctcgatcg cgctccaatc cgaccaaacc aacaaagttc
      361 tcgggctcgc gacgagagcg ggacggacga tcaaggcgag cttacgcgcc gccggcatgt
      421 cgacaggatc ggccgcgacc aacgtcgcgt aatcgcagcc cttggcgatt gcttcgacca
      481 gcgggctttc cgcggcgccg tcctcgccga gcaggtcctg cgtcaggttt tggatctgag
      541 ctccatggct ggtcagcctg ccggtttgcc cggcgccggc gaagcggtat tgccctcgta
      601 acacgccatc gacttgttgc gcttccaggc gtgcgaactt ttttggtgaa gctccagcgc
      661 cgaaaaggtg cagcgccgcg gcctcatgcg ccttcatctc attagggcta aggccgccgt
      721 ttgcgcgctt gatgtcgagc atcgcgagca cgcgggcaac acgatcacgt gtcaggctga
      781 gctcctgcgg ctcgtcctcg tcgccgttat cagcgtcgtc gtcatcgccg tcatcagccg
      841 gaacgccgac catcaagatt tcacgcattg ccgcgtcggc aaggttgtcg tgcagccagg
      901 ttgcaatctt tttcgcttgt gtgacgcggg tgacagctcc atcggtcagc acgaccaacc
      961 ggccgccgat ggcgacggca tcctcggccg cgagcgctgc ggcgcggcgc acgtagggca
     1021 tgttgagcgc cacgcctcgc cggttgacgt gctcgaacgc ccagtactgc tgccactcct
     1081 cgtgcggcag cggccgggtc cggcgataga cctcgcgcat cgcctcgatg tcctggcgcg
     1141 cgtaggcgag aaagcgctcc cattccgcgg ggtattcgtg cgggttcgca ccttcgatgc
     1201 agaacaattt aattaaccgc ttgccatcct tttgtttgcc ggcgccgccg aggtagcggg
     1261 aagcgctttc gagatcagtc ggcaggttag agacgccggc ctgaaccatc ggatcgatga
     1321 tgcgttccgg cgtgaggaaa ggaaatccga gcgtggcgta attccaaaca gcggtgtcga
     1381 aactcgcgtt ccacgcggcg aaggttgcgc cgtgctcgaa ggcattgcga agctcgtctg
     1441 gcgcgtgatc ccaatcgaga attgcgctgt cggcgtgcca ggttcgagcc ggcacgttgc
     1501 cgattgcgta ggccagaacg atggcatgcg tcgatgccgc ggcgacgtat cggaatggtc
     1561 cggcagcctt caagtcgact tcgggagctg cagcctcgaa gtcgatccag acgatatcgt
     1621 cgactttgaa acatcgtgat ttcctgagtc tcagaaaatc aggcgaggcc gccgcgcggc
     1681 cggcctcgcc ttgatcaagc gtgcctcgat ttacgagacg cgccggcgcc gtggctgctc
     1741 gacgggcggc gatgccgacc ccggcgctgg tgccggtgcc ggcgttggcg ccgggccgtc
     1801 aagcggcatc caatccacga ctgtcattac ggggatccag accttcccgt attgactgtg
     1861 cggataagag tctttttcga ggaacacgat cggcgcgatc tggccgtaat gctgaccgct
     1921 gttaagccgg tcgcgtacca gctcaaccaa tccggcgaca gcttgagtgc cgccgacggt
     1981 ggacgctttg aaggtcactt caacaccggc atcggtgccg tcgagacact tcatgttcac
     2041 ggcccattgc tcctgccacg ggaagcccgt gtcaggtaac gtcgtgatac cgggcttcgg
     2101 ctggctgacc gaaacgaggc gctcggtcgg cttgttggcc tcatcgaaac agatgaagcc
     2161 ccatttgaag gtcgtcgggt tgacagccca tctgctgcct ttctccggca cgatgcgctt
     2221 ctgtccaaac gaccaggtgc cgtcaccttc ccgtttgaac tgcatcatcg gcaggcctga
     2281 acgaccggcg atggacgctg tatcgacgcg gttgagtacc gtctggagcg ccgtcaggga
     2341 agtgagcgca cctcccgctg ctggcgcggg ggcaagcttg tccccgagcc tttcgtgttc
     2401 gccattatcg ttggcgtgat cgcggtcgcg atcatggtcg tggtcgttac tcatagttac
     2461 ctatctcctg tgtttgccaa tgtcgttggc ggcgtgatcg cggtcgcgat catgattgtg
     2521 atcttggttc atggttgcct ccctcctgga aggctgcgag cgcctcggag aatgttcgcg
     2581 caagctcgac ccgtccgggc accgggacgt gcgcgttttc actccgcacc agcgagacac
     2641 cggagcggcg tgaaacgatt aattctgctg gaaccttcag gccgcgcgct tttgcgcgca
     2701 gttcaacttg tttgggcgag cgtatggttt cggcgatgac atcgccgcgg gcgagaccga
     2761 ggcggatcag cgcgccgatc gccgcgtcct cgttgtgcca gtgccgctcg gcacggccgg
     2821 ctgagagcgc gtaacccggc acgtggtcgc cttcctccaa tgcgcgcttc gcttgatcat
     2881 gcagcgctgt gcgaaggtct ttgatcgcgt cgaccagctc caagccgtag gcgagcatct
     2941 gcaagtaggc ttcctttgtg gtcgacgacg gggacatcgc gaaatgcgcg agatcgagca
     3001 gcggaccggt gtgcaccgga cagatcggcc gagccgggca gaagcggcag tgagtgccca
     3061 gctgcaagcg cggcgcactg gagagcgcct cctcgcaggc gctgcgatag gccgcgacga
     3121 acgcgtccag ctcggcatgc gtcaccgcga ccgaggagac catttcggca tcgagctcga
     3181 tcgacatcgg ctgcaggatt gtcagtgcga tctcgtcgac gccggcgaaa aattctggca
     3241 acgaatgacg ggcggccgcg gcgtagaaca ggagctgcgc gttgatgacg tcttcgtcgc
     3301 cctccggaga gagtgcgaga acgcgcacgc cgacgccaaa tttgaagtca atcacataga
     3361 tcttgcggct gatgcggatg agcagatcgc aggtgccaaa ggtgtcgacg atagcgggaa
     3421 agatgacgcg ccgttcgaga tagtattcag ctccgggcgc atccaggagc gtctccacgt
     3481 aggcgtatgc cggccacaga gcggtctcga cgtcgtcgtg cgtgatcgtg tagctgccga
     3541 acgttttgcc ggcgaggctc tcgacgaggg catgcgtgtt ttcggcgagg aggcaggtta
     3601 tcgccgcatg gagcgcggtg ccgcggtcgg cataggccga aactttccgc agatgcggag
     3661 ggacttttcc gacgaggccg acggatgccg ggcagcgcag aatgcgcgcc gcgacgctgc
     3721 cgccgaaggg cgaatgagcc ggctcgcgtg ggagctcgac gttcatggtg cagcctcttc
     3781 cgccgcggtg ataacttcgc gctgcgtggc ccgtgaattc gaggcaactg catcgagc
//
LOCUS       DATLJT010000810         1671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2119351,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000810 DATLJT010000000
VERSION     DATLJT010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119351"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..434
                     /locus_tag="VM910_18335"
     CDS             162..434
                     /locus_tag="VM910_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64517.1"
                     /translation="MARKPATRVEFTLFDVVYEDGSLRSNRRVPSDVLGGLDGDAAAR
                     AVIEEQDRIIAEKSGTPAVGVKSIHRSGSKDIDRTIGPSRRELASD"
     gene            complement(643..897)
                     /locus_tag="VM910_18340"
     CDS             complement(643..897)
                     /locus_tag="VM910_18340"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI64518.1"
                     /translation="MVAAANLVEVAAPVGDTARATMLSALMGGQSLTATELAYCANVS
                     RSTASGHLSKLDQPHLVGRSTAADAGGDLAAFLGGAEELF"
     gene            1050..1343
                     /locus_tag="VM910_18345"
     CDS             1050..1343
                     /locus_tag="VM910_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI64519.1"
                     /translation="MVAYWVARSKVNNPDQYKKYTDLVPGILRKFGGKILARGGKFKI
                     LEGPEKFHRFIVIEFPSFEKAVACHESPEYLEAAAHRKKDGAGELEIVIVESI"
     gene            complement(1379..>1671)
                     /locus_tag="VM910_18350"
     CDS             complement(1379..>1671)
                     /locus_tag="VM910_18350"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI64520.1"
                     /translation="ALGAFVAISNHPDVMLKMMLEPGDFQFINNRTILHGRAGFEDHA
                     AKDQRRHLYRLWLNVPQWPRMPASQSTLVAADMARWDAMAAQHRAATPAWAS"
BASE COUNT          341 a          509 c          520 g          301 t
ORIGIN      
        1 gcctcgcgac tatatatgtg cacgcgttcg agttttgccc gtcgttgcca ttgatttgcc
       61 cgcccaggcg ctggggcaat ttggtcttgc cggccgtggc tcgatgatcg cgatgccgcg
      121 cttgacgcgt ggcagcgttg aacaatgaca ggaaaaccaa aatggcacgg aaaccggcaa
      181 cgcgcgttga attcacgctt ttcgacgtgg tttacgaaga cggctcgctg cgttcgaatc
      241 gcagagtgcc gagcgacgtc ctcgggggcc tcgatggcga tgccgctgcg cgtgccgtca
      301 tcgaagaaca ggaccggatc atcgcggaga agtcagggac tcccgccgtt ggcgtgaaaa
      361 gcattcaccg gtcgggcagc aaggatatcg atcggaccat aggaccgtcc cgacgtgaat
      421 tagcatcgga ttgatcggtt cttctacagc cgaagtttta gacaggcgta gccatccctt
      481 taggctatcg gctacgaggc aggacggcga ttcggtgctg gctcagtgcc ggctttatgg
      541 caagcgatat caaagcgatg tctcacaagg aagacttgcc ggagaaggag acacccgaga
      601 gcgcgccgga ccgcccgctg ctcgatttct ccgacgctgc agtcaaaaaa gctcctccgc
      661 accgccaaga aacgcggcta ggtccccccc cgcgtctgcg gcggtggacc ggccaaccaa
      721 gtgcggctgg tccagcttgc tcaggtggcc gctcgccgtc gagcgagaga cattggcgca
      781 gtaagcaagc tcagtggccg tcagcgactg gccgcccata agggcgctaa gcatcgtcgc
      841 tcgcgcagtg tcccccacgg gagcggccac ttccaccaga tttgccgccg cgaccatcct
      901 taatcttgtt gctcataccg aaagcatatc aatcgcaaac ggttcggcat gggtcgaact
      961 gttcgcgtga gacaattgcg aacctcgcag caagaatgcg ctgtagccct agtctagcaa
     1021 gggcgttcac ggttggcatg gaggatgaga tggtcgccta ttgggtcgcg cgctcgaagg
     1081 tcaacaatcc cgatcagtac aagaagtaca ccgacctggt accaggcatc ctccgaaagt
     1141 tcggcggcaa gatcctggcg cgcggcggca agttcaagat cctcgagggc ccggagaaat
     1201 tccaccgctt catcgtcatc gagttcccga gcttcgagaa ggcggtcgcc tgccacgagt
     1261 cgcccgaata ccttgaggcc gcggcgcacc gcaagaagga cggcgcgggc gagttggaga
     1321 tcgtcatcgt cgagagcatc tgagacccac cgacacgcct aggcccggcc ccgctaggtt
     1381 aagacgccca ggccggcgtc gccgcgcgat gctgcgccgc cattgcgtcc cagcgcgcca
     1441 tgtcggcggc caccagcgtg ctttgagacg ccggcatgcg cggccactgc ggcacgttga
     1501 gccacagccg atagagatga cgccgctgat ccttcgccgc atgatcctcg aagccggctc
     1561 ggccgtgcag gatcgtgcgg ttgttgatga actggaagtc gcccggctcg agcatcatct
     1621 tcagcataac gtcgggatgg ttggagatgg ccacaaaggc accgagcgcg g
//
LOCUS       DATLJT010000811         2448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1660587,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000811 DATLJT010000000
VERSION     DATLJT010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660587"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VM910_18355"
     CDS             complement(<1..382)
                     /locus_tag="VM910_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HVI64521.1"
                     /translation="MLRCRRAFLAFAIILATPLAAAAQGTGTAPPPSTGAPPPQAAAT
                     PPPQSDELLKPEQLDALVAPIALYPDALIANMLAAATYPLEVVTAARWVKDHKNLKGD
                     QLKAEVDKQSWDDSVKALTSTPSVL"
     gene            596..916
                     /locus_tag="VM910_18360"
     CDS             596..916
                     /locus_tag="VM910_18360"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HVI64522.1"
                     /translation="MRPDLLLTNAYDELMANVLDAKHIGQGVVDGVECEHLAFRGTDT
                     DWQIWIETGARPIPRKYVITSKTLAGAPQYIKDWNTDAIADANAFVFKPRQVPQRRAL
                     VRRT"
     gene            1014..1249
                     /locus_tag="VM910_18365"
                     /pseudo
     CDS             1014..1249
                     /locus_tag="VM910_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1332..1994)
                     /locus_tag="VM910_18370"
     CDS             complement(1332..1994)
                     /locus_tag="VM910_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64523.1"
                     /translation="MTTKQSGLTRAQLRTPRAAAVAGILFSVLLLAVFWLMRLSVPAD
                     PHEPGAWLRGGVTYVALALNLVPFAGAAFLWFVGALRDKLGAREDQFFATVFLGSGLL
                     VLAMLFAAAAVVGAIIIAFHSAPEALIDSATFHFGRGLAYGMVNIYVVKTASIFMITT
                     STIALYTQLTPRWLAIGGYVVAVVLLIGSYYLDWSILVFPLWVLLVSICILLDKEKEP
                     LA"
     gene            complement(2057..>2448)
                     /locus_tag="VM910_18375"
     CDS             complement(2057..>2448)
                     /locus_tag="VM910_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI64524.1"
                     /translation="LKPGMVAEAACTSLPWTIIPMVITSVQDYIAAGQFRGGEQLVDP
                     SLARPGTILVFLEPLAKNGLADVTAGSSCIVNSYTSNHEKIASKDTGALHGFALHAID
                     AVGLVHALLLRIQTLLLPIKTLVLSGH"
BASE COUNT          506 a          741 c          780 g          421 t
ORIGIN      
        1 cgagcacgct cggcgtactc gtcagcgcct tgacgctatc gtcccaggac tgcttgtcga
       61 cttcggcctt cagttgatcc cccttcagat tcttgtgatc cttgacccag cgggctgctg
      121 tgaccacctc cagaggataa gtcgcggctg ccagcatgtt ggcgatcaat gcgtcgggat
      181 agagcgcgat cggcgcgacc agcgcgtcga gttgctcggg cttcagcaac tcgtcggatt
      241 gcggcggcgg cgtggcggcg gcttgcggag gcggcgcgcc ggtggatggc ggcggtgcgg
      301 tgccggtgcc ttgcgcggcc gctgcaagcg gtgtcgcaag gatgatcgcg aaagccaaaa
      361 atgctctcct gcaacgcaac atggccaatc tccgttggga ccttacccgt cagccaatcc
      421 cgacttgcga accggcactt gattaagatc aaagctccgg ccacgaaagg atgttcggcg
      481 catcgcgtga gaggcgacag ttgaatgttt cgtgaaatcg cgagagccgg acgaagtgat
      541 ctcctcgtct ctgcccttga tctaggtcaa gcaaacttct cgccgcgatc cgagtgtgcg
      601 gcccgatctg ctcctgacca acgcatatga cgaattgatg gcaaacgtcc tggacgccaa
      661 gcacatcggc cagggcgtgg tggacggcgt cgaatgcgag catctcgcgt tccggggcac
      721 cgatactgat tggcagatct ggatcgagac gggcgcgcgg ccgatcccgc gaaaatacgt
      781 gatcacgagc aagacgcttg caggcgcgcc gcaatacatc aaggactgga acaccgacgc
      841 catcgctgat gcaaacgcgt ttgttttcaa accgcggcag gtaccgcaaa ggcgagcctt
      901 agtccggaga acatgagcga attcgacgaa atcccgccgg gcacagcccc aggagcaaag
      961 aaatgacggt cagatcacgc aaggccatca atctcatcgt ggcggcggtc gccatgttag
     1021 gcggcctgct gctggacgga gacagcgcaa tcttcggttc gctcgtctcc tccgccgaag
     1081 cacgcgtggg acgcccgttg acgccgatga gttacgctgg cgtcgctcgc cgaacgacgc
     1141 gtcgcgccgt taaggcaccg ccgccgctgc cgcggccggc gcctatgtgg cgacgccggg
     1201 gtgcaggcag gtggtcgatg cgtatgggcg ggtctacacg caatgctagc gaggcgagtg
     1261 ccgtctagca gcgaccgcag ccacgcttca agggcgcgat cggaagcatg aacggtgagc
     1321 gtacgcgccg ctcatgcaag cggctccttt tccttgtcca gcaggatgca gatgctgacg
     1381 aggagcaccc acagcggaaa caccaggatg ctccagtcca ggtagtagct tccgatcagg
     1441 agcaccactg cgaccacgta tccaccgatt gccagccaac gcggcgtcag ttgcgtgtac
     1501 agtgcgatgg tcgatgtcgt gatcatgaag atggaggccg tcttgaccac gtagatattg
     1561 accatcccgt aggccaggcc ccggccaaaa tgaaacgtcg ccgaatcgat cagggcttcg
     1621 ggagcggaat ggaaggcgat aatgatcgcg ccaaccacgg ccgcggcagc gaaaagcatg
     1681 gcgagcacca gcaggccgct tcccaggaac accgtcgcaa agaattgatc ctcgcgcgcg
     1741 ccgagcttgt cgcgcagcgc tccgacgaac cagaggaacg cggcgccggc aaacggcacc
     1801 agattgagcg caagggccac gtaagtcacg cctcccctga gccacgcgcc gggctcgtgg
     1861 gggtcggccg gtaccgaaag tcgcatgagc cagaatacgg cgagcagcag cactgaaaac
     1921 aatatgccgg ccactgcagc agccctcggg gttcgcagtt gtgcgcgggt caatccagat
     1981 tgtttggtcg tcatgacaaa gctcccggcg cgacgccgtc ttcaatgccc gatcgctgcc
     2041 gatagcggcc tggttatcag tgaccgctca gcaccagtgt cttgattgga agcagcagcg
     2101 tctggatgcg cagcagcagc gcgtggacca gtccgacggc atcgatggcg tgcagcgcga
     2161 aaccatgaag cgcgccggta tccttcgaag caatcttttc gtggttgctg gtgtaggagt
     2221 tgacaataca ggagctgccg gccgtcacat ccgccagacc gttctttgcg agcggttcaa
     2281 gaaacacgag aattgtgccg ggccgcgcca gactcggatc gacgagctgc tcgccgccgc
     2341 gaaactggcc ggcggcgata taatcctgca cgctcgtgat gaccatcggg atgatggtcc
     2401 atggcagcga cgtgcaggcg gcctcggcga ccatgccggg cttgagaa
//
LOCUS       DATLJT010000812         5399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1200835,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000812 DATLJT010000000
VERSION     DATLJT010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200835"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VM910_18380"
     CDS             complement(<1..428)
                     /locus_tag="VM910_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI64525.1"
                     /translation="MYRHILIPTDGSELAEHAVTNGLSLAKFVGAKVTVIIVEDPFDW
                     LSVPETRASQRQSLDALAKHNEQVKKHAESVLSRAVDAAKQAGVPCDTMQVGNARPYQ
                     AIIAAASDRGCDLIVMASHGRGGLSAVVLGSVTNKVLTHT"
     gene            complement(1203..2501)
                     /locus_tag="VM910_18385"
     CDS             complement(1203..2501)
                     /locus_tag="VM910_18385"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HVI64526.1"
                     /translation="MSTTISIIGGVGLFLLGMTVMTAGLKGLAGSGLRTVLSNAAATP
                     LRGAFWGGVATLLVQSSSATTMTTIGLVSAGLLTFPQGLGLVFGANVGTTGTGWLVAL
                     IGVRVSLTAAALPMIFVGALLKLLGGGRVSAAGAAFAGFGLVLFGLTTLQEGMGGLAE
                     RLHPADLPAVLAAPGVAWWSSLLGVLVLVAVGLVMTTVMQSSTAAISVTLSAQYAGAI
                     GLDQGCALIIGQNIGTATSSAMAAIGASSTAKRLALAYVLFKVIAAVIALLLFPVLTP
                     ILISASHTVDGVTLLAGYHTAYNLVGVLVLLPLIDQFTRFVERMLPERTSALTRCLDP
                     AALATPIAAVEAVRRTVAGALIALCRSLDSGLQAASRGEAVRPGKDDVSVPAAADALR
                     QARVFMSDVSWATGVGGRAAAPHEYLARTGARVSPGRGGG"
     gene            4396..4557
                     /locus_tag="VM910_18390"
     CDS             4396..4557
                     /locus_tag="VM910_18390"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVI64527.1"
                     /translation="MLADATQLPILVDGDTGYGNFNNVRRLVRKLCERHIAGVCIEDR
                     LFFQDQLLH"
     gene            4675..>5399
                     /locus_tag="VM910_18395"
     CDS             4675..>5399
                     /locus_tag="VM910_18395"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI64528.1"
                     /translation="MDVQCALDCGSLLGEGPVWDLGEQRLYWVDIKRRQIHRFAPDTG
                     ADETWPMPEDIGALAPREGGGLVVALRSGFHFYDLIDRRLVPAALPEDEPEHNRFNDG
                     KADRQGRFWAGTMDEGEKLPTGGLFRLQPNLACHKMIDGIICSNALCWSPDSRVMYYA
                     DSGQRTVWAWNFDAATGEIDNRRVFVRIAAADGVPDGATVDSEGFVWIAHWGGWRIVR
                     YDPAGRVARTIMLPVAQPTCPMFG"
BASE COUNT         1034 a         1727 c         1643 g          995 t
ORIGIN      
        1 ttcgtgtgcg ttaggacctt gtttgtcaca ctgccgagca cgactgcgga cagcccgccg
       61 cgcccgtgcg atgccatgac gatgagatcg cagcccctgt ccgaggccgc tgcaataatg
      121 gcttgatagg gtcgcgcgtt ccctacctgc atcgtgtcgc agggcacacc ggcctgctta
      181 gctgcatcga ccgctcgact tagcacactc tcggcgtgct tcttgacctg ttcgttatgc
      241 ttggcgagtg catcaagtga ttgccgctgc gacgctctcg tttctggaac gctgagccag
      301 tcgaaggggt cctcgacgat aattacggtt accttggctc ccacgaattt cgccaatgac
      361 aatccatttg tcactgcatg ctccgccaac tccgacccat ctgttggaat gagaatatgc
      421 cggtacatcc gccacctctc gattgctggg cgattgcgtc agatatccga actgtgcgtg
      481 caatttcttg agcaaaatga atttatccgt caatcgctgc cgccgtcgca gtatcgatcc
      541 aaaggtttag catcaacttg acgaagccgg caccgagcag gacgtagacc aggacgttgt
      601 tgaactgcga gaggaaccgc atgcttcgct cgcattcaac cctttcttgg tatcagtggt
      661 cagcccttcc gtgacctgtc gcgcggtcat agcgtgccag gccgatgcat cgcttgcgga
      721 ttccgccttg gacgtgcctt ccatgggcat cctccttttg cgcgggcgag gcgctttgat
      781 cggctgctgt gttaagcgcg gccaacgagg tgcgctgcgg agcgccaagc gtgatgcgca
      841 aacgcgccga ggcgccggac cgcatcgacg cgagccatcg cctcgccgcc cgtgagcgct
      901 ccacccgcga ctgcgctgag cgtcgcgctg cgatacgcgc gctgcagctg gtccaatgtt
      961 ttcgcgcagt gctcgagccg cataaaagct tcctccgtcg aagccgcggg gggtcgaagg
     1021 tttcagcgcc cggtaattcg cgcaccacac gccgcgcctc gatcggggtg acgtggtcgt
     1081 caccaacgtt tggcgcggca acttcgcggg cagctacgac tgcgcttcgc atggcatccg
     1141 cgcacagccg cgaagcgcgc acgtcgtcag gcgctccgtt cagcgcggtg aattcgactt
     1201 tgtcacccgc cgcctcggcc aggcgagacg cgtgctccag tgcgtgcaag gtattcgtga
     1261 ggcgccgctg ctcgtcctcc gactccggtg gcccaactca catcggacat gaatactcgt
     1321 gcttggcgca acgcgtcggc ggccgccggc accgacacgt catctttacc cggccggacg
     1381 gcttcgccgc ggctcgcggc ctgcagtcca ctgtcgagcg atctgcacag cgctatcaat
     1441 gcgcccgcca ctgttcgccg taccgcttcc acggccgcaa taggggttgc gagcgccgca
     1501 gggtcgaggc agcgcgtgag cgccgatgtt cgttccggca gcatgcgctc gacaaaccgc
     1561 gtgaattggt cgatcagcgg cagcagcact aggacgccga ccaggttgta cgccgtgtgg
     1621 tacccagcca gcagcgtcac gccgtcgacg gtatgcgagg cgcttataag gatcggcgtg
     1681 agcacgggga acagcagcaa cgcgatcacg gcggcgatca ccttgaataa cacgtaggcg
     1741 agggccaacc gcttggccgt gctgctcgcg ccgattgctg ccatcgcgga actggtggcg
     1801 gtgccgatgt tctgcccgat gatcagcgcg cagccctggt ctagcccaat cgctcccgcg
     1861 tactgagcgg agagcgtcac cgagatggcc gcggtcgacg actgcatcac cgtggtcatg
     1921 accagcccga cggcgacgag aaccaacacg ccgagtaaac tcgaccacca cgcgacgccg
     1981 ggagcggcga gcacagccgg caggtctgcc gggtgcagcc gttctgccag ccctcccatc
     2041 ccctcctgca gggttgtcag tccaaacagg accagcccga atccggcgaa cgccgcgccc
     2101 gccgcggaca cgcgcccgcc acccagcagc ttgagcaacg cgccgacgaa gatcatcggc
     2161 aatgcggcgg ctgtgagcga aacgcgcacg ccgatcagcg caacgagcca cccggtgccc
     2221 gtggtgccca cgttcgcgcc aaacacgagc cccaggccct gcggaaacgt caacaggccc
     2281 gcgctgacca ggccgatggt agtcatggtc gttgcgctgg atgactgtac aaggagcgtg
     2341 gcgacgccgc cccagaaagc gccgcgcaac ggtgtcgccg ccgcgttgct gagcactgtg
     2401 cgcagcccgg agccggccag ccccttcagt ccggcggtca tcacggtcat gccgagcaga
     2461 aacagtccca cgccgccgat aatcgagatc gtcgtcgaca tctattttct tacgaacgac
     2521 caaacggcgt tcattgtagc gcagcctcaa cgcggaacca ggaacgaaag ggtattccct
     2581 tcaccggctt ccagacatcg gaacaatcca gctgacaacg gcagcctgac cgaaattttg
     2641 caaatagttc gccgagtggg tggcgtgccg cgcaacaaaa agcggcccct catgtctaca
     2701 tgggttactt attagcgtct gagcgactag agccgatcac gaaccgaagc gcttacctct
     2761 taaagcggac cggtgtgccg ccacggcgcc cgcttccccg aatgaaaccg aaagcgccgg
     2821 ctgtagtgag cgtgagggct tgagcggagg aggagccccg gcatttagcc tgggttgtcc
     2881 cgtgtactcg cgatctagtc cggatgcttt gacctcaggt cgaccgtagg gcagatccgt
     2941 ccgcttactg acgcaatttg tactgacgca attggtcttc gtagcccgga tatagcagag
     3001 cgagatccgg cctcaccgcg gcatcgactt tcttagtgat gtgtaagaac tagattggga
     3061 taaccccgcg ttgcggacag atcaaacttg gcgtccggct gcacattcgc gctgaaccag
     3121 gtttgagcta ggtggttctg gttatcgggc gcagctaatc aatgcgataa tagaacagcc
     3181 gtctgcagcg tggattgaaa gatatcccgc gttgcgctac cgaacagaaa ctctcgctct
     3241 ctggcatgtc cgaaacctcc catcaccacg agagccgctt cgatctacgc ggcacggccg
     3301 agaagaacgt caccaacatt tcgactgccg cgctgcaata gttcgaagcg cgcctgcaca
     3361 ccccatcggg agagataacg gcagactttg tctccagaac gaacagctcg cagctccttg
     3421 tcatccgcca ctgtcacgac taccacttcc cgcgcaatgg gtcagtatcg gcaaagcatc
     3481 gtgcaaggac cgaaccgccg accgcgtgcc gtcccaggcg acgagggctc tttccgctgc
     3541 tggcgtatga acgctcggcg gcacgaggat gatcgggtga ccactcccga atagcacacc
     3601 tccacgatcg ccaccgccct gcatgagatc gggtgcgctc atcggagcgg gtgcgtcagg
     3661 ctcgatttcc gttatcaaaa cggtgacatg tgcttcaaag aggccggcca aaacgaacgc
     3721 atagttggcg gcttctccga aagcgttcgc atatgcaggc gtaatgatcg caagcacgtc
     3781 cttcatgctg cccctgtgcg gtcgcgctca tctcaagtcg accatgcaaa tctggttatg
     3841 ccaatttcgg ttcgcaagcg ggcaagccga tcagggctcc cgaaacaata aagcgtcgcg
     3901 cagatcacag cacgtcgcga aatgaaatgt gcaaatcatc tatgcttctc gacgatgatc
     3961 gctcgcaacc cgtccagtgc aggccctcgc gttgcatcgt cagctgcccg agtacaccac
     4021 gtagactgga taggagaatt gagatgctcc cggcacgaga tggactcgcc ctgacgctag
     4081 gtgaggctcg agtgcactca ttctgaagta gccggatcgt gcctagacaa catcgcagcg
     4141 agagcgcgag gtaggggtcg tgagttcgcg ccaggcgttg ccgtcgtcgt atcgcgccga
     4201 tagtcggccg ccgttcgacc gcgcggctcc gttgcgggcg gagatttact cgccctttct
     4261 caccttcctt gttgggggcg cacagcggcc tctcggccaa gatcgtggaa gaagcgggtt
     4321 tcgcagcatc tgggcctcgg gcttgacaat ctccgcgagc ctcagcaacg aggcgtcctg
     4381 gacccaggtg ctcgaatgct ggccgacgcc acgcaattgc ccatcctcgt agatggcgac
     4441 accggctacg gcaacttcaa caacgtgcgc cggctcgtgc gcaagctgtg cgagcgtcac
     4501 atcgccgggg tctgtattga ggacaggctc tttttccaag accaactcct tcattgacgg
     4561 cagaagactc catgttgcca gcgcaggaag cggcaaggcc ggagccgcgg gcaggccggc
     4621 tcagtcggca acccggcagc ggctgcttgc gtagcagagg ggagggatgg cgagatggat
     4681 gtgcaatgcg cgctcgattg tggttcgctc ctcggcgagg ggccggtctg ggacttgggg
     4741 gagcagcgcc tctactgggt cgacatcaag cggcggcaga tccatcgctt cgcgccggac
     4801 accggcgccg acgagacctg gccgatgccg gaggatatcg gcgcgctggc accgcgcgaa
     4861 ggcggcggtc tcgtcgttgc gctgagatcc ggcttccact tctatgatct catcgatagg
     4921 cggctggtcc cggcggcgct gcccgaggac gaaccggagc acaaccgctt caatgacggc
     4981 aaggccgatc gccagggccg gttctgggcc ggaaccatgg acgagggcga aaagctgccg
     5041 accggcggcc tgttccgtct ccaacctaat ctcgcctgcc acaagatgat cgacggcatc
     5101 atttgctcca atgcgctgtg ctggagcccc gacagccgtg tcatgtacta cgccgacagc
     5161 gggcagcgca ccgtctgggc gtggaatttc gacgcggcaa ccggcgagat cgacaatcgc
     5221 cgcgttttcg tccggatcgc cgcggcggac ggcgttcccg acggcgccac cgtcgacagc
     5281 gagggtttcg tgtggatcgc gcactgggga ggttggcgca tcgtccgcta cgatcccgcg
     5341 ggtcgcgtcg cccggacgat catgctgccg gttgcgcagc cgacctgtcc gatgttcgg
//
LOCUS       DATLJT010000813         4765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3038790,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000813 DATLJT010000000
VERSION     DATLJT010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038790"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..659)
                     /gene="efp"
                     /locus_tag="VM910_18400"
     CDS             complement(93..659)
                     /gene="efp"
                     /locus_tag="VM910_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HVI64529.1"
                     /translation="MRVIASSIRKGNVIEQDGKLYVVLSAENIHPGKGTPVSQIEMRR
                     ISDGVKISERYKTTDQVEKATVEERNFTYLYEDADGFHFMNPETYDQVQVSKDVVGGG
                     AAYLQENMTVKLSLHEMNPIAVQLPQRATLEVVETEPVTKGQTASSSYKPAMLSNGLR
                     TAVPPHITVGTRIVVMTEDGSYVERAKD"
     gene            965..2017
                     /gene="epmA"
                     /locus_tag="VM910_18405"
     CDS             965..2017
                     /gene="epmA"
                     /locus_tag="VM910_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HVI64530.1"
                     /translation="MAASNPFSPFWAASRHADRRPFLTARAAIMRTLRAWFEAEGFSE
                     VETAILQVSPGNETHLHAPRTTLTGPDGARATRYLRTSPEFACKKLLAAGETKIFEFA
                     KVFRDRERGDLHLPEFTMLEWYRANAAYDAVMADTVAVIAQAARATRTGQFAFRGKSA
                     DPFAEPERLTVAAAFARHAGVDLLATISGSEGDRAALAQAAAGKVRLAEDDTWSDIFS
                     KILVEHVEPKLGQGRLTLLCEYPAPEAALARAKPGDPRVAERFEVYACGVELANGFGE
                     LTNAGEQRRRFGLAMDEKQRRYGERYPLDEDFLAAVAKMPDASGVALGFDRLVMLASG
                     ASRVDQVIWAPPAGET"
     gene            2014..3075
                     /locus_tag="VM910_18410"
     CDS             2014..3075
                     /locus_tag="VM910_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HVI64531.1"
                     /translation="MSGRNFARTLREPADLVAHGLARPADLSVLEKVASRYAVAITSD
                     MAALIDPDEPDDPIARQFVPTADELVTEKSEHADPIGDHSHSPVTGIVHRYPDRVLFK
                     LVHVCAVYCRFCFRREMVGPGKEGALSEEAYADALDYIRAHREVWEVILTGGDPLMLS
                     ERRLARITTDLAAICHVRIIRIHTRIPVADPDRITDRMVAGLKVKGATTWVALHVNHA
                     RELTDKARASIARLVDAGIPLVSQSVLLRGVNDNPTALEDLMRAFVECRVKPYYLHHG
                     DLAPGTAHLRTTLAHGQALMRQLRGRVSGLCQPDYVLDIPGGAGKSPVGPNYLEAQDS
                     LRYRVTDYCGEVHLYPPQP"
     gene            3110..3355
                     /locus_tag="VM910_18415"
     CDS             3110..3355
                     /locus_tag="VM910_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64532.1"
                     /translation="MKKTIISMALLVLIGAPGAFAQSGKGTSVGTTINGAVPQAPVGH
                     RQPRASDVPNDKNVSSDPVSKEDAMLDKKIKSICRGC"
     gene            3356..>4765
                     /locus_tag="VM910_18420"
     CDS             3356..>4765
                     /locus_tag="VM910_18420"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI64533.1"
                     /translation="MRVSARPCIDRRRFLRQAAAAGLTVGTWTHRAIAQEVPLAERLA
                     RYATGLRYEDLPEDIVRLAKRTILDTVGCAFGGYAAAPSKIALRLAGGVSAKQPATVL
                     ISGIETSPDLAVFADGVMIRYLDFNDAFVSLTHGAGHPSDTIAALLTAAELHRRSGRD
                     LITATVLAYEVFCKIADVFDYLGNGIDHSTITGIAATVGAGRLMGLTGDQLVQAIGIT
                     VGGNTATRQGRADALSNWKAFAAADACRKAIFSLELAQDGMTGPANVFEGRYGFFKVM
                     GRKEVAPVQLGEPFGIRRAFTKRFPLGQFAQTVAQAAAEARPFLDSAENIQEVNINVS
                     RSAIKIMADGPDKWRPQTHETADHSIPYAAGLVLMYGKIEPEYYEDPYLHDTRLLELI
                     GRIKCLPSEEADRSEQEFNLCELELVLKSGARKTFRVEYHRGHFKNPMTDAELEEKFR
                     LMAEKYLPADRVDNLLRLLW"
BASE COUNT          900 a         1480 c         1489 g          896 t
ORIGIN      
        1 gagcaggaag aaagccgcca gcaaccgcgt gaccgaaccg actgctctcc ccgtcaccct
       61 ccgcccccgg cgcagaaata ccgattccca gtttaatcct ttgcgcgctc gacgtaagag
      121 ccatcttcgg tcatcaccac aatccgcgtg ccgaccgtaa tgtgtggcgg gaccgcggtc
      181 cgcaatccat tcgagagcat ggcgggcttg taggacgagg aggcggtctg gcccttggtc
      241 accggttcgg tctccacgac ctcgagtgtc gcgcgctgcg gcaactggac ggcaatcggg
      301 ttcatctcgt gcagcgacag cttgaccgtc atgttctctt gcaaataggc ggcgccgccc
      361 ccgaccacgt ccttggaaac ctggacctgg tcataggtct cgggattcat gaaatggaag
      421 ccgtcggcgt cttcataaag ataggtgaaa ttacgctcct ccaccgtcgc tttctcgacc
      481 tggtcggtgg tcttgtagcg ttcggagatc tttaccccgt cgctgattcg acgcatttca
      541 atctggctga ccggcgttcc cttgccggga tgaatgtttt cagcgctcaa aacgacataa
      601 agcttgccat cttgctcgat gacattgccc ttgcgaatag aacttgcgat gactctcaat
      661 gccttatttc ctgatgttct ggcctgccac cgaacaggac ttgcgggtat tgcggcgatg
      721 gacgcggttt cgggccgcaa catactgatt ttgccgacca ttgctagtgc cgccgatttg
      781 aagtccccgc gcggcaggcg gctaatccga gccacggacg tcacatcgaa aagcggcact
      841 acaatcatat gtttgcgagt gcccatggct ttccgaagat cgctcgatgc gagcaggccg
      901 cgcggcgatt gcgaacttcg gaaacgggca ttcgtatgtt agggaacctc tggacacgcg
      961 tttgatggcc gccagcaacc cattttcgcc attctgggcg gcgagccggc atgccgatcg
     1021 caggccgttt ctgacggcac gagccgcgat catgcgcacg ttgcgggcgt ggttcgaggc
     1081 cgaaggattc agcgaggtcg aaacggccat tttgcaggtt tcgccgggca atgagaccca
     1141 cctgcacgcg ccccgcacca cattgaccgg ccccgatggt gcgcgggcga cgcgatatct
     1201 gcggacctcg ccggaattcg cctgcaagaa actcctggcc gccggcgaaa cgaaaatctt
     1261 cgaattcgcg aaagtgtttc gcgaccgtga gcggggtgac ctgcatctgc cggaattcac
     1321 catgctggag tggtaccgtg ccaatgccgc ttacgacgcc gtgatggccg acacggttgc
     1381 cgtgatcgcg caggcggcgc gcgcgacccg gactgggcaa ttcgcgtttc gcggcaaaag
     1441 cgccgatccc tttgccgagc cggagcggct gacggttgct gccgcattcg cgcgtcatgc
     1501 cggcgtcgat ctgcttgcaa ccatttccgg aagcgagggc gaccgtgcgg cgctggcgca
     1561 ggccgccgcc ggcaaggtgc gcctcgccga ggacgacaca tggtcggata ttttcagcaa
     1621 gatcctcgtc gagcacgtcg aaccaaagct ggggcagggg cgtttgaccc ttttatgcga
     1681 atatccggca ccggaggcgg cgctcgcgcg cgcaaaacca ggtgatcccc gcgtcgccga
     1741 gcgcttcgag gtctatgcct gcggggtcga actcgccaat ggatttggcg aattgaccaa
     1801 tgcgggggaa cagcgccgcc gctttggcct tgccatggac gaaaagcagc gccgctatgg
     1861 cgagcggtat ccgctggacg aggatttcct tgcggcggtt gcaaagatgc ccgatgcaag
     1921 cggtgtcgcg ctcggtttcg accgcctggt gatgctggcg agcggcgcct cgagggtcga
     1981 tcaggtgata tgggcgccgc ccgcaggtga aacatgagtg gacggaactt tgccaggact
     2041 ttgcgcgaac cggccgacct ggttgcccac ggcctggcgc ggccggctga cctttcggtg
     2101 ctggaaaagg tcgcctcccg atatgcggtg gcgatcacgt ccgacatggc agccctgatc
     2161 gacccggacg agcccgacga cccgatcgcg cggcaattcg ttccgaccgc cgacgagctc
     2221 gtgacggaga aaagcgagca cgccgatccg atcggcgatc acagccattc gccggtcacc
     2281 ggaatcgtgc accgctatcc cgatcgcgtg ctgttcaagc tcgttcacgt ctgcgcggtc
     2341 tattgccggt tctgcttccg ccgcgagatg gtcggacccg ggaaggaagg cgcgctgtcg
     2401 gaggaggcct acgccgacgc gctcgactat atccgcgcgc acagggaagt ctgggaagtg
     2461 attttgaccg gcggcgatcc cttgatgctg tcggaacgac ggctcgcgcg aatcacgacc
     2521 gatctcgccg caatctgtca cgtcaggatc attcgcatcc atacccgcat tcccgtggcc
     2581 gatcccgatc ggatcactga tcgcatggtg gcgggcctca aagtcaaggg cgcgaccacc
     2641 tgggtcgcgc tgcacgtcaa ccatgcccgc gaattgacgg acaaggctcg cgcgtcgatc
     2701 gcgcgcctgg tcgatgcagg cattcccctt gtcagccagt cggtgctgtt gcgcggtgtc
     2761 aacgacaatc caaccgcgct tgaagatctc atgcgcgcct ttgtcgaatg ccgcgtcaag
     2821 ccctattacc tgcatcacgg cgacctcgcg ccgggcacgg cgcacctgcg cacgacgctc
     2881 gcgcacggcc aggcgctgat gcgacagttg cgaggcagag tttccgggct atgccaaccc
     2941 gactacgtgc tggacatccc cggcggcgcc ggcaagtcgc cggttggccc gaactatctg
     3001 gaggcgcagg actctttgcg ctatcgtgtt accgactatt gcggcgaggt gcatctctat
     3061 ccgccgcaac cgtgaagacg tgatcaattt tgaagacggg aggagtgaca tgaaaaagac
     3121 gatcatctcg atggcgctcc tggttctgat cggcgcgccc ggggcttttg cacagagcgg
     3181 caagggaacg tccgtgggca cgaccatcaa tggcgccgtc ccgcaggctc cggtcggcca
     3241 tcgtcagccg cgggccagcg acgtgccgaa cgacaagaac gtgtcgagcg atccggtctc
     3301 caaggaagac gcgatgctcg acaaaaagat caagagcatc tgccgagggt gctagatgcg
     3361 cgtgtcggcg cggccgtgca tcgatcgccg ccgtttcctg agacaggccg ctgcggcagg
     3421 cctgacggtg gggacctgga cccatcgcgc gattgcgcag gaggtcccgc tcgcggagag
     3481 gctcgcccgc tatgcgaccg ggctgagata tgaggatctt cccgaagaca tcgtccggct
     3541 cgccaagcgt accattctgg acactgttgg atgcgcgttc ggtggctatg ccgcggcacc
     3601 gagcaagatt gcgctcaggc tcgccggcgg cgtcagcgca aaacagcccg cgacagtcct
     3661 gatcagcggc atcgaaacga gtcctgatct cgcggtgttc gccgatggcg tgatgatccg
     3721 atatctcgac ttcaacgacg ctttcgtcag tctcacccat ggcgccgggc acccgagcga
     3781 tacgatcgcg gcgttactga ccgcggccga gttgcaccgg cgcagcggcc gcgatctcat
     3841 taccgcaacg gtgctcgcct atgaggtgtt ctgcaagatc gccgatgtct tcgattatct
     3901 cggtaacggc atcgaccatt cgacaatcac tggtatcgcc gcaacagtcg gcgcaggccg
     3961 cctgatggga ctgaccgggg accaactcgt tcaagccata ggcatcaccg tcggcggcaa
     4021 caccgcaacc cgccagggtc gcgcggatgc gctctcgaac tggaaggcgt ttgctgcagc
     4081 cgatgcgtgt cgcaaggcga ttttttcgct ggagcttgca caggacggga tgacggggcc
     4141 cgccaatgtc ttcgaggggc gctacggctt tttcaaggtg atgggccgca aggaggttgc
     4201 gccggtgcaa ctcggcgaac ccttcggcat tcgccgcgcc ttcaccaaac gcttcccgct
     4261 cggtcaattc gcccaaactg ttgcgcaggc ggccgcggag gcccggccgt tcctggacag
     4321 cgcggagaat atccaggagg tcaacatcaa cgtttcgcgt tccgcgatca agatcatggc
     4381 cgatgggccg gacaagtggc ggccgcagac gcacgagacc gcggatcaca gcattcccta
     4441 tgcggcgggc ctcgtgctca tgtatggcaa gatcgagccg gagtattacg aagaccccta
     4501 tctgcacgat acgagactgc tcgaactgat cggccgcatc aaatgcctgc cgtcggagga
     4561 ggccgaccgc agcgagcagg agttcaatct ctgcgagctt gagctcgtcc tgaaatcagg
     4621 cgcacgcaag acgtttcgcg tcgaatatca ccgagggcat ttcaagaacc cgatgaccga
     4681 tgccgagctc gaagagaagt tccggctgat ggcggaaaag tatctgcctg cggatcgcgt
     4741 cgacaatctg ctgcggctgc tctgg
//
LOCUS       DATLJT010000814         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3268785,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000814 DATLJT010000000
VERSION     DATLJT010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3268785"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VM910_18425"
     CDS             <1..687
                     /locus_tag="VM910_18425"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVI64534.1"
                     /translation="MRKIQPNLPLRLRGPSRVVQDGRPMVDIVYVLTNEAMPGLIKIG
                     CTNVDLAARIKQLYSTGVPLPFELFYACEVKDGPSVEGKLHDAFGDHRISKSREFFRL
                     APERAKAALSLAEIREVKLGDEIFETNEIKAEVEAAKRRSRFRFSMIGVAPGTELQLA
                     KNPTIICTTVDDGNKVDYMGDITSLSDAALQAYNALGESWPSVSGPWAWTYKGKRLDD
                     LRREIEDQSD"
     gene            complement(733..1575)
                     /locus_tag="VM910_18430"
     CDS             complement(733..1575)
                     /locus_tag="VM910_18430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64535.1"
                     /translation="MNGLRIRSMRPDEISIAVSWAAAEGWNPGLADDLCFGAADPDGF
                     FIGELDGAPAATVSCVNYGEKFAFLGFYIVREDLRGRGYGLRIWDAAINHAGSRVIGL
                     DGVVAQQENYRKSGFKLAYANVRYGGTVEAPAAPRGAVIALSDLPLAAVEAYDATVFP
                     APRTAFLRCWIGTSAHVGRALLRDGALAGWGVIRPCRKGHKIGPLFADDRAAAEAVLS
                     ALLASVGEGEVFLDVPSINRDAIALAEGLGLAPVFETARMYTGAIPHLQPERVFGVTS
                     FELG"
     gene            complement(1700..>3235)
                     /locus_tag="VM910_18435"
     CDS             complement(1700..>3235)
                     /locus_tag="VM910_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64536.1"
                     /translation="RAIPAEIGGGFGGKTIVYLEPVATLLAKKSGRPVKMVMTREEVM
                     RATGPTSGSKSTVKIGATRDGKITAAQGTFYLQAGAFPGSPIRGAAGCSFAPYDIANI
                     LSVGFDVCSNRSKVAAYRAPGAPIGAYAVECVMDEVASALKMDPLEFRLKNAAREGTK
                     AAHGPVFPRIGYVETLQVAKEHPHYAAPLGQTPGKPRGRGVASGFWFNAGGESSAQVN
                     ITEDGNVVVTTGHPDIGGSRAGIANVCAELLGIDYRRISVIIGDTQTVGFSNLTGGSR
                     VLFASSMVVTQSTEKVIQTLRERAAKIWGIDPEAVKWENGAAHPVSPNAGQFEPITLE
                     QLAAKAPEQGGPIGAGVQLNTQGAEGGFGTHICDVEVDTELGIVRVIRYTAVQDVGRA
                     VHPGYVEGQLQGGVAQGIGWALNEEYIYTKDGKVDNPGFLDYRMPVCSDLPMIDTALV
                     EVPNPKHPQGVKGVGEVPLVPVMAAVANAVHNALGLRFDSLPMSPPKVAAALEGMVRQ
                     AAE"
BASE COUNT          613 a         1064 c          995 g          563 t
ORIGIN      
        1 atgcgcaaga ttcagccaaa tctcccgctc aggttacgcg gaccatccag agtagtgcag
       61 gacggaagac cgatggttga tatcgtttat gttctgacca acgaagccat gcccggactg
      121 atcaagattg gctgcacaaa cgtcgatctg gcggcgcgca tcaagcaatt gtattcgacc
      181 ggcgtgccgc tgccgtttga gctcttttac gcctgcgaag tcaaagatgg cccctcggtt
      241 gaaggcaagc tgcatgatgc gtttggcgac caccgcatca gcaagagccg cgaatttttt
      301 cgacttgctc ccgagcgcgc caaggctgcg ctttcgcttg ccgaaatccg ggaggtcaag
      361 ctcggcgatg agatattcga gaccaacgaa atcaaggccg aggtcgaagc cgccaagcgt
      421 cgcagccgct ttcgtttttc gatgatcgga gtagcgccgg gaactgagct tcagcttgcg
      481 aagaatccaa cgatcatttg cacgaccgtc gacgacggca acaaggtcga ctacatgggc
      541 gacatcacct cgttgtcgga cgcggcgtta caagcctaca acgcgctggg ggaaagttgg
      601 ccttcggtgt ccggtccctg ggcctggact tacaagggca aacgcctcga cgatctgagg
      661 cgggaaatcg aggaccagtc ggactgatat cggcggtctg acgcgctcga tgacgggatg
      721 gcaagccccg cgctatccca actcaaagct cgtgacgccg aacacccgct caggctgcaa
      781 gtgcgggatc gcgccggtat acatgcgcgc ggtctcgaac accggcgcaa gccccaagcc
      841 ttccgccaac gcgatggcgt cgcggttaat gctgggaacg tcgaggaaga cctcgccttc
      901 gcccacgctt gccagcaagg ctgacagaac cgcctctgcg gcggctcggt cgtcggcaaa
      961 cagcgggccg atcttgtgcc ccttccgaca tggacggatc acgccccagc ctgcaagggc
     1021 gccatcacgc aggagcgcac gaccgacatg cgccgacgtg ccgatccagc agcgcagaaa
     1081 cgccgtgcgc ggcgccggga acaccgttgc atcgtacgcc tccacggccg ccaaaggcag
     1141 gtcgctgagc gcaatgacag cgcctcgcgg cgcggccgga gcctcgacgg tgccgccata
     1201 acggacattg gcgtatgcca gcttgaaccc ggacttccgg tagttctcct gctgagccac
     1261 caccccgtcg agcccgatca cacgcgagcc ggcgtggttg atggctgcgt cccatatgcg
     1321 caggccgtag ccgcgaccgc gcaagtcctc tcgaacgatg tagaagccga ggaaggcaaa
     1381 cttttcgccg tagttgacgc aagagacggt cgcggccggc gcgccgtcga gctcaccgat
     1441 gaagaaacca tcgggatcgg ccgcgccgaa gcacaggtcg tcggcaaggc ccgggttcca
     1501 gccttcggcc gcggcccagc tcacggctat cgaaatttca tccggtcgca tggatcggat
     1561 gcgcaagccg ttcatggcgg ttgacaccca acaatggcat tgatgacgtg acgggtttcg
     1621 cttctgcctt cgctcgcacg agcttcgacg gacaagtgcg ctcaacccat cctacagacc
     1681 atcgatcgaa ggtccgaccc tactccgccg cctgacgcac catgccttcc agcgccgccg
     1741 caaccttcgg cggcgacatc ggcaggctgt cgaagcgcag tccgagtgca ttgtgcacgg
     1801 cgttggcgac cgcagccatc acgggtacca gcggcacctc gccgacgccc ttgacgccct
     1861 gcggatgttt cgggttcggt acctcgacaa gcgcggtgtc gatcatcggc aggtcggagc
     1921 agaccggcat gcgataatcg agaaagccgg gattatcgac cttgccgtcc ttcgtataga
     1981 tgtattcctc gttcagcgcc cagccgatgc cttgcgcgac accgccctgg agctgaccct
     2041 cgacatagcc aggatgcacg gcgcggccga catcctgcac cgccgtgtag cggatcacgc
     2101 gcacaatgcc gagctcggta tcgacctcga cgtcgcagat atgcgtgcca aagccaccct
     2161 ccgcaccctg ggtgttgagc tgcacgccgg cgccgatcgg cccgccctgc tccggcgctt
     2221 tcgccgcgag ctgctcgagc gtgatcggtt cgaactggcc ggcgttgggg ctcaccggat
     2281 gtgccgcgcc gttctcccat ttcaccgctt cgggatcgat accccagatc ttggccgcac
     2341 gctcgcgcaa ggtctggatc actttctccg tcgactgcgt caccaccatg gatgaggcaa
     2401 aaagcacgcg gctgccgccg gtcaggttgg agaagcccac ggtctgcgta tcgccgatga
     2461 tcacggagat gcggcgataa tcgatgccga gcagttcggc gcagacattg gcaatgcctg
     2521 cacgcgagcc gccgatatcg gggtggcctg tggtgacgac cacattgccg tcttcggtga
     2581 tgttcacctg cgcggaagat tcgccgccgg cattgaacca gaagcccgaa gccaccccgc
     2641 ggccgcgcgg tttgcccggc gtctgaccga gcggcgccgc gtagtgcggg tgttccttgg
     2701 cgacctgcag ggtctcgaca tagccgatgc gcgggaatac cgggccgtgc gcggccttag
     2761 taccctcacg cgccgcattc ttgaggcgga actcgagcgg atccatcttc agcgcgcttg
     2821 cgacctcgtc catcacgcac tcgaccgcat aggcgccgat cggcgcgccc ggcgcgcgat
     2881 aggccgcaac cttcgagcgg ttcgagcaga cgtcgaaacc gaccgagaga atgttcgcga
     2941 tatcatatgg cgcaaagctg cagcccgcgg cgccccggat cggcgagccg ggaaacgcgc
     3001 cggcctgcag atagaaggtg ccctgggcgg ccgtgatctt tccgtccctg gtcgcgccga
     3061 tcttgaccgt gctcttcgaa ccggacgtag gcccggtcgc acgcatcacc tcctcgcggg
     3121 tcatcaccat cttgaccgga cggccggatt tcttcgcgag cagcgttgcg accggttcga
     3181 gatagacgat ggtcttgccg ccaaagccgc cgccgatttc ggcagggatg gcgcg
//
LOCUS       DATLJT010000815         6401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1661201,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000815 DATLJT010000000
VERSION     DATLJT010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661201"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VM910_18440"
     CDS             complement(<1..179)
                     /locus_tag="VM910_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045861.1"
                     /note="catalyzes the reaction of formaldehyde with
                     glutathione to form S-hydroxymethylglutathione; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     protein"
                     /protein_id="HVI64537.1"
                     /translation="MSVHIHPSVDNGVKKGTGNFAGGTLVCKCKDRPVKVAVKGDVAH
                     NHACGCTKCWKPEGA"
     gene            complement(211..1320)
                     /locus_tag="VM910_18445"
     CDS             complement(211..1320)
                     /locus_tag="VM910_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HVI64538.1"
                     /translation="MKTRAAVAFEAKKPLEIVELDLEGPKPGEVLVEIKATGICHTDA
                     YTLDGFDSEGIFPSILGHEGAGIVREVGAGVNSVKPGDHVIPLYTPECRQCKSCLSQK
                     TNLCTAIRATQGKGVMPDGTSRFSYRGKTIYHYMGCSTFSNFTVLPEIAVAKIREDAP
                     FNTSCYIGCGVTTGVGAVVNTAKVTPGANVVVFGLGGIGLNVIQGAKMVAADKIIGVD
                     VNDSKEEWGRRFGMTHFVNPKKVSGDLVQHLVTLTDGGADFTFDCTGNTTVMRQALEC
                     CHRGWGVSVIIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMNGK
                     IQIDPMITHVLKLEDINKGFDLMHEGKSIRSVVVY"
     gene            complement(1331..1849)
                     /locus_tag="VM910_18450"
     CDS             complement(1331..1849)
                     /locus_tag="VM910_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome, methanol metabolism-related"
                     /protein_id="HVI64539.1"
                     /translation="MRIICLTAAAAILIAAGPAAFADGSGDPTAVKSEDGKYFDKEGN
                     PTFKIAPDGMVDWYTYSGYRRYHSECHVCHGPDGMGSTYAPALKDSLKTISYADFLGV
                     VASGRKNVNTAQENVMPAFGDNPNVACYMDDIYVYLRARANDAVGRVRPAKHEDKSAA
                     YADAENACMGKH"
     gene            complement(1927..3810)
                     /gene="xoxF5"
                     /locus_tag="VM910_18455"
     CDS             complement(1927..3810)
                     /gene="xoxF5"
                     /locus_tag="VM910_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthanide-dependent methanol dehydrogenase
                     XoxF5"
                     /protein_id="HVI64540.1"
                     /translation="MRKVLLASCLGSVAAFVGAGASANDQLIKMSQNPKDWVMPSGDY
                     ANTRYSKLNQITAANVGKLQVAWTFSTGVLRGHEGGPLVIGNMMYVHTPFPNKVYALD
                     LSQEQKIIWMYEPKQNPDVIPVMCCDTVNRGLAYSDGKIFLHQSDDKLIALDAKTGNL
                     VWSATNGDPAKGQTGTSAPLVVKDKVLVGTSGGEFGVQCSMTAYDVKDGKQVWKAYSE
                     GPDDQTLVDEKATELGKPVGKDSSVKTWQGDQWKIGGGCTWGWLAYDPQLNLVYYGSG
                     NPSTWNPKQRPGDNKWSMTIFARNPDTGVAKWVYQMTPHDEWDYDGVNEMILTDQQIG
                     GQARKLLTHFDRNGLAYTLDRTTGELLVAEKFDPKVNWTSGVDMNKSSPTYGRPKVVD
                     QYSTDKQGEDHNVKGICPAALGSKDEQPAAYSPETQLFYVPTNHVCMDYEPFKVSYTA
                     GQPYVGATLSMYPPPGESNMGNFIAWDARTGKIVWSNKEQFSVWSGALATAGGVVFYG
                     TLEGYLKAVDAKTGKELYKFKTPSGIIGNVTTYEHNGKQYIAVLSGVGGWAGIGLAAG
                     LLAPDNAAAWQRAAGRAAAGGALDTAERNVETAGLGAVGGYAGLSNFTALGGTLTVFA
                     LPQ"
     gene            4166..5128
                     /locus_tag="VM910_18460"
     CDS             4166..5128
                     /locus_tag="VM910_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI64541.1"
                     /translation="MSETIRSLSTWGMGPKKQIQFWSDALTDLCGKFDVDPLEASSLE
                     GRINYTTVSRLKLCQIEASQHRIAHTVAQSQGSEHPYFKILFQTQGVSYFEQNGCQIQ
                     VTPGDCLAYDVSCPHTIISPSQTRHEVVIVPKELLRERGFHFDKMYACKLSARAGTGR
                     IAHDLVHAAFDEATKLSPNSAVSVAESLMDLLLLPLREADTTFDRAGPEAMYVRAQAF
                     IREHLRDPDLCIDQISAELGCTKRYLHMLFSERGMTVSDYIWRARLQHCRQELEAHAG
                     KTITDVAFSWGFSSSSHFSRVFRKCFGVVPSAIHKGRHASPEVS"
     gene            complement(5140..5391)
                     /locus_tag="VM910_18465"
     CDS             complement(5140..5391)
                     /locus_tag="VM910_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64542.1"
                     /translation="MGQLHSALLIALLLIGFLSCARAEDAPGAAKAGPSATTTATGTA
                     KERLGPKWSDEQRVDNCKVPIDKRGTKPRPSSCPGDAGE"
     gene            5530..5931
                     /locus_tag="VM910_18470"
     CDS             5530..5931
                     /locus_tag="VM910_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HVI64543.1"
                     /translation="MISRRTMLMVPAALLAQGSSLALAAPRLDPVKAFDTDADGTLDL
                     AEVKKAASALFAKLDRDHDGTLDKRELAGRLSAKELAAADPDHDGTLTLDEYLSVVEQ
                     RFKAANPDSDGTLDAKELSTPAGRALLRLLR"
     gene            6033..6113
                     /gene="pqqA"
                     /locus_tag="VM910_18475"
     CDS             6033..6113
                     /gene="pqqA"
                     /locus_tag="VM910_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HVI64544.1"
                     /translation="MTWKTPKIVEVQVGMEINMYACAVRK"
BASE COUNT         1154 a         1964 c         1923 g         1360 t
ORIGIN      
        1 atcgcgcctt ccggcttcca gcacttcgtg catccgcagg cgtggttgtg tgcaacgtcg
       61 cccttgacgg cgaccttgac cggccggtcc ttgcatttgc agacaagagt tccgccggcg
      121 aaattgccgg tgcccttctt cacgccgttg tcgaccgatg ggtggatatg aacgctcatg
      181 ggcataacct ccttgtgttt gacgttatgg ctaataaacc acgaccgaac gaatcgactt
      241 gccttcatgc atcaagtcga agcccttgtt gatgtcctca agcttgagga cgtgggtgat
      301 catcgggtcg atctggatct ttccattcat gtaccagtcg acgattttcg gcacgtcggt
      361 gcgtccgcgc gccccgccga aggcggtgcc tttccacacg cgaccggtga cgagctggaa
      421 cggacgggtg gcgatttcct ttccggcctc cgcgacgccg atgatcacgg agacgcccca
      481 gccgcgatgg cagcactcga gcgcctggcg catcacggtg gtattaccgg tgcagtcgaa
      541 ggtgaaatcg gcgccgccgt cggtgagcgt caccagatgc tggacgaggt cgccgctgac
      601 cttctttgga ttgacgaaat gggtcatgcc gaagcgacgg ccccattcct ccttcgaatc
      661 attgacgtcg accccgatga tcttgtccgc ggcgaccatc ttggcgccct ggatgacatt
      721 caaaccgatc ccgccgagcc caaagaccac gacattcgca cccggcgtca ccttggcggt
      781 gttgacgacg gcaccgacgc ccgtcgttac gccgcagccg atgtaacagc tcgtgttgaa
      841 gggcgcgtct tcgcggatct tcgcgaccgc gatctcgggc agcaccgtga agttcgagaa
      901 cgtcgagcag cccatatagt ggtagatggt ctttccccga tagctgaagc gcgaggttcc
      961 gtcgggcatc acgcccttgc cctgggtggc gcggatcgcg gtgcaaagat tggtcttctg
     1021 gctcaggcag cttttgcact ggcggcattc gggcgtgtag agcgggatga catgatcgcc
     1081 cggcttcacc gagttcacgc ccgcgccgac ctcgcggacg atcccggcgc cttcgtggcc
     1141 gagaatggac gggaagattc cctcgctgtc gaagccgtcg agcgtatagg catccgtgtg
     1201 acagatgccc gtcgccttga tctcgaccag aacttcgccg ggcttcggtc cttccagatc
     1261 gagctcgacg atttccagcg gcttctttgc ttcgaaagcg acggcggcgc gtgtcttcat
     1321 ggtcggaccc tcagtgcttg cccatgcacg cgttttcagc gtccgcatag gccgcggatt
     1381 tgtcttcatg ctttgccggc cgcacgcgtc ctaccgcgtc gttggcgcgc gcgcgcagat
     1441 agacgtaaat gtcgtccatg tagcaggcga cgttcggatt gtcgccgaac gccggcatta
     1501 cgttctcctg cgccgtgttg acgttcttgc ggccgcttgc gaccactccc aggaagtcgg
     1561 cgtagcttat cgtcttgagc gagtccttca gcgcgggggc ataggtcgat cccattccgt
     1621 ccggtccgtg gcagacatgg cattccgaat gatagcggcg gtatccggaa taggtgtacc
     1681 agtcgaccat gccgtcgggt gcaatcttga aggtcggatt cccttcctta tcgaaatact
     1741 ttccgtcttc ggacttgacc gcggtcggat cgcccgaacc gtcggcgaag gcggctggac
     1801 cggcagcgat aagtattgcc gctgcggccg ttaaacaaat gatgcgcaag agcttgtcct
     1861 cgactgtcga tcccaggtga tccggcgcgt ggataagacc cacgcgccgg cacgaggttc
     1921 gtcagctcat tgcggcagcg cgaacactgt gagtgttccg ccgagcgccg tgaaattgct
     1981 cagccccgca tagccgccga cagcaccgag accggcggtc tccacgtttc tctcggcggt
     2041 gtccaatgca ccacccgccg ctgcgcgacc ggcggcgcgc tgccatgctg ccgcattgtc
     2101 aggggccagc aaccccgccg ccaggccgat accggcccag ccaccgacgc cggacagtac
     2161 cgcgatgtac tgcttgccgt tgtgttcata cgtcgtgacg ttgccgatga tgccggatgg
     2221 agtcttgaac ttgtagagct ccttgccggt cttggcgtcg accgccttca gatagccttc
     2281 gagcgtgccg taaaacacca cgccgccggc ggtcgcaagc gcgcccgacc agaccgagaa
     2341 ctgctccttg ttcgaccaga cgatcttgcc ggtcctggcg tcccaggcga tgaagttgcc
     2401 catgttgctt tcgcccggag gcggatacat cgacagcgtc gcgccgacat agggctgacc
     2461 tgcggtgtag ctcaccttga acggctcata gtccatgcaa acgtggttgg tcggcacgta
     2521 gaagagctga gtttccggcg agtaggctgc cggctgctcg tccttcgagc cgagtgccgc
     2581 cgggcagatg cccttgacgt tgtggtcctc gccctgcttg tcggtcgaat actgatcgac
     2641 gaccttcggc cggccatagg tcggggagct cttgttcatg tcgacgccgc tggtccagtt
     2701 gaccttcgga tcgaactttt cggccaccag cagttcgccc gtcgtgcggt cgagcgtata
     2761 cgccagcccg ttgcggtcga agtgcgtgag aagcttgcga gcctgaccgc cgatctgctg
     2821 gtcggtgagg atcatttcgt tgacgccgtc atagtcccac tcgtcatggg gcgtcatctg
     2881 atagacccac ttggccacgc cagtgtccgg attgcgcgcg aagatggtca tcgaccattt
     2941 gttgtcgccc ggacgctgtt tcggattcca ggtcgaggga ttacccgagc cgtagtagac
     3001 gagattgagc tgcggatcat aagcgagcca gccccatgtg cagccgccgc cgatcttcca
     3061 ctggtcgcct tgccacgtct tcacactcga gtccttgccg accggcttgc cgagttcggt
     3121 ggccttctcg tcgaccaggg tctgatcgtc cggtccttcg gagtaggcct tccacacttg
     3181 cttgccgtcc ttgacgtcgt aggcggtcat actgcattgc acgccgaact cgccgccgga
     3241 cgtgccgaca agcaccttgt ccttgaccac gagcggcgcc gatgtgccgg tctgtccctt
     3301 ggccggatcg ccgttggtcg cgctccagac gagattgccg gtttttgcgt cgagcgctat
     3361 cagcttgtcg tcggactgat gcaggaagat cttgccgtcg gaataggcga ggccgcggtt
     3421 cacggtgtcg cagcacatta ccgggatcac gtccggattt tgcttcggct cgtacatcca
     3481 gatgatcttt tgctcctgag aaaggtcaag cgcgtagacc ttgttcggga agggcgtgtg
     3541 cacgtacatc atgttgccga tgacgagcgg tccgccttcg tggccgcgca gcacgccggt
     3601 cgagaatgtc catgcgacct gcagcttgcc gacattggct gcggtgatct ggttcagctt
     3661 ggagtagcgt gtattggcat agtcgcccga tggcatcacc caatccttgg ggttctgcga
     3721 catcttgatg agttggtcat tggctgacgc gccggcaccg acgaatgcag ccacagaccc
     3781 aagacagctt gcgagtagca ccttgcgcat cttgcatcct cccacttgaa gctgtgggct
     3841 tagagagccc actttccgtt atcttagcac gtatccctat ccagttcgac ggattgagac
     3901 ttgcccaaga gcgaaaccga tttgctcaga agagaaacgc ggcgagagtt ttttgcccgc
     3961 ttgggtggcg atattgcgga gcatcaaacc ttggggcatc ttgcccacat cgaggttttc
     4021 cttgacgggg tcgaaactta gttcgaggat tgccaacggg aaatgaggag gagcgcccgc
     4081 aattcccgat ttccgcctca aattgaatgt cgcttgccgt gcgcagccgc aggcgcacgg
     4141 ttgatctcaa aatcggtggc tggaaatgtc cgagacgatc cgctcactca gcacttgggg
     4201 catgggcccg aagaagcaga tccaattttg gtcagatgcg ctgactgatc tctgcggcaa
     4261 attcgacgtc gatccgctcg aggcctccag cctggaaggg cggatcaatt acaccaccgt
     4321 ttcgcggctg aaattgtgcc agattgaagc aagccagcac cggatcgccc acaccgttgc
     4381 acaaagccag ggcagtgagc atccgtactt caagattctg ttccagaccc agggcgtttc
     4441 ctatttcgag cagaacggct gccagatcca ggttactccg ggcgattgcc ttgcctacga
     4501 cgtgtcctgt ccgcacacga tcatcagccc gtcccagacg cgtcatgagg tcgtcatcgt
     4561 tcccaaggaa cttttgcggg agcgcggctt ccactttgac aagatgtatg cgtgcaagct
     4621 ttcggcgcgc gccggcacgg gccgaatcgc gcacgacctg gtgcatgccg cgttcgacga
     4681 agccaccaag ctttcgccga acagcgcggt cagcgtcgcc gaatcgctga tggatctttt
     4741 gctgttgccg ctccgcgagg ccgatacgac gttcgaccgg gccggtccgg aagcgatgta
     4801 tgtcagggcg caggcgttca ttcgcgagca tttgcgcgat ccggatcttt gcatcgacca
     4861 gatttccgct gagcttggct gcaccaagcg ctatctgcac atgctgttca gcgaacgcgg
     4921 catgaccgtc agcgactaca tctggcgtgc caggttgcag cactgccgcc aggaactgga
     4981 agcacatgcg ggaaagacga tcacggacgt ggccttttcc tggggttttt caagctcttc
     5041 gcatttcagc cgcgtcttcc ggaaatgttt tggggtcgtg ccgtccgcca tccacaaagg
     5101 gcggcatgcg tcgcccgagg ttagctgaca gcttgcatct cactcgccgg catctccggg
     5161 gcaactgctc ggccgcggct tggtgccgcg tttgtcgatc ggcaccttgc agttgtcgac
     5221 gcgctgttcg tcgctccatt tcgggccgag cctttccttg gctgttcccg tcgctgtggt
     5281 cgttgcagac gggccagcct ttgccgcgcc tggtgcgtcc tccgcgcgcg cacaagacaa
     5341 aaagccgatc aacaacagcg cgataagcag tgcgctatgt agttgtccca tggttttctc
     5401 ccgcctgtcg ctgtgcgatc acgcaaacta cgctatttaa cgatgtcgaa agtcggctgg
     5461 ccacccgaaa cctgaaagcc ggaacagcgg ttcaggtgat ttcgcaacag ggatcaatgg
     5521 gagatcaaga tgatatcgcg ccgaaccatg cttatggtgc ctgcggcgtt gttggcgcag
     5581 gggagttcgc ttgcgctcgc ggcgccccgc ctcgatccgg tgaaggcatt cgataccgat
     5641 gccgacggca cgctcgacct tgccgaggtg aagaaagccg cttcggcatt gttcgcaaag
     5701 ctcgatcgcg accacgatgg aacgctcgac aagcgtgaat tggccggaag gctgagcgcc
     5761 aaagagctcg cggcagccga ccccgatcac gatggaacgc tgacgctgga cgaatatctt
     5821 tccgtcgttg agcagcgctt caaggcggcc aacccggaca gcgacgggac gctggacgca
     5881 aaagagctga gcacgcccgc cgggcgagcg cttcttcggt tgttgagata gcgcgcctcg
     5941 tcacgaaact tgcgcgagca cttgcatgaa gcgcgcgaca gccctacgtt gccggcggcg
     6001 cgagagttgc gccaaatgct tgggagatcc aaatgacctg gaagactccg aagatcgtcg
     6061 aagtgcaggt gggcatggaa atcaacatgt acgcctgcgc ggtgcgcaag taatcgcacc
     6121 agacgctctg cccggctccg gcattaatct gccgtgggcc gcggcagggc cttcgcctca
     6181 atcctcgacc aggcgatctc ccgaacatcc cccgcgtcag acgtcacgcc gcatcagcgt
     6241 cgctcgcgcc gcggcgatct ctcaaggcca agttttccgt ttgaaacggc aaagtcggcg
     6301 tgctcgcgcc tcggcttgtg ctaggctttc gccggcgtga caggcgctcg gtcaagacca
     6361 acggccttcg gaacgggaat ggctcttcct gcaaaatcgg t
//
LOCUS       DATLJT010000816         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4188288,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000816 DATLJT010000000
VERSION     DATLJT010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188288"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VM910_18480"
     CDS             <1..691
                     /locus_tag="VM910_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HVI64545.1"
                     /translation="NYTPFGHSDWQTIVADIKKFGSAGKKTAVVSTINGDANVPFYKE
                     LGNQGIKATDIPVVAFSVGEEELAGIDTKPLVGHLAAWNYFESIKTPANEKFIKEWQA
                     YTKNPKRTTNDPMEAHVIGFNMWVKAVEKVKSTDPDKVIDALPGTEVPNLTGGISKML
                     PNHHITKPVFIGEIKANGQFDVVWKTPGLVPGDAWSKELEGSKDLIGDWVGKKCGNYN
                     VKTNKCLGSGT"
     gene            937..2547
                     /gene="urtB"
                     /locus_tag="VM910_18485"
     CDS             937..2547
                     /gene="urtB"
                     /locus_tag="VM910_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HVI64546.1"
                     /translation="MPSNVLHCLRALALAVVLTAFALPALAGPFEDAVGKFANDDFSD
                     TEEAIGVVAASGNPLAYPIISALQEERLMADPDTKKVYIQGTDGKNIDAATGAAVASI
                     PDSASAVRLNNRLRRTVEAALGGLTLLSPDPATRIQAAQSVFKSHEEAALPTVESALA
                     KETDANAKIALSGARAAILLFKSDASEADKLDAVATIRARGDQEAMALLTGLGSEQPP
                     AVAKAAAAAISSIESSLAIWSAVQNAWYGLSLGSVLLLAAIGLAITFGVMGVINMAHG
                     EMVMLGAYATFVVQEVIRTRYPQAFDYSLLIAVPLAFLVSGAVGILIERSIIRFLYGR
                     PLETLLATWGLSLVLQQAVRTAFGPTNREVGNPSWMSGAFELGQITITYNRLWILCFT
                     LAVFAILLAMLRFTALGLEMRAVTQNRRMAASMGIATSRVDALTFGLGSGIAGIAGVA
                     LSQIDNVSPNLGQSYIIDSFMVVVFGGVGNLWGTLVGAFTLGIANKFLEPVAGAVLGK
                     IAILVLIILFIQKRPRGLFALKGRAVEA"
     gene            2544..>3154
                     /locus_tag="VM910_18490"
     CDS             2544..>3154
                     /locus_tag="VM910_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HVI64547.1"
                     /translation="MMPHVLTRSLDRGATIFILVVAACGILIPLSNLLLPAGSFFQVP
                     TYLVALWGKYVCYAILALSIDLIWGYCGILSLGHGAFFALGGYAMGMYLMRQIGTRGV
                     YGNPILPDFMVFLNYPRLPWYWNGFDMFWFAALMVVLVPGLLAFCFGWFAFRSRVTGV
                     YLSIITQAMTYALLLGFFRNDFGFGGNNGLTDFKDILGFNVQA"
BASE COUNT          547 a         1003 c          959 g          645 t
ORIGIN      
        1 caactacacc ccgttcggtc acagcgactg gcagacgatc gtggccgaca tcaagaagtt
       61 cggctcggcc ggcaagaaga ccgctgtcgt ctcgaccatc aacggcgacg ccaacgtccc
      121 cttctacaag gagctcggca atcagggcat caaggcgacg gacatcccgg tggtcgcgtt
      181 ctcggtcggt gaagaagaac tcgccggcat cgacaccaag ccgctggtcg gccaccttgc
      241 cgcgtggaac tatttcgagt cgatcaagac gcccgcaaac gagaagttca tcaaggaatg
      301 gcaggcctac accaagaatc cgaagcgcac taccaacgac ccgatggagg cccacgtgat
      361 cggcttcaac atgtgggtga aggcggtcga gaaggtgaag tcgacggatc ctgacaaggt
      421 gatcgatgcg ctccccggca ccgaagtgcc caacctgacc ggcggcatct cgaaaatgct
      481 gcctaaccat cacatcacca agccggtgtt catcggcgag atcaaggcca acggccagtt
      541 cgacgtggtc tggaagaccc cgggcctggt ccccggcgac gcctggtcga aggaactgga
      601 agggtccaag gacctgatcg gcgactgggt cggcaagaag tgtggtaact ataacgtcaa
      661 gaccaacaag tgcctggggt cgggcacctg accttgtgcc ccggccctga cattcgcgcc
      721 atgttgcagg aacgccattt gcgaatgtca gggtcaaaaa ggggtacgtg caaaatatga
      781 ttctggtgcg gttttggatt tgacattcgc tacgaggacg caccgcttca cagaggcgaa
      841 cgtcaaatcc accgcgctgg tcgccgcgcc gacaacaaga gaacgaagaa ggcggcggtg
      901 ttgccgcctt cttttacctt ctgccggggt tgttcagtgc cgtccaacgt ccttcattgc
      961 ctccgcgcgc tcgcgctcgc cgttgtcctg actgctttcg cgctgccggc cttggctggt
     1021 ccgtttgagg acgctgtcgg caagttcgcc aacgacgatt tctccgatac cgaggaagcg
     1081 atcggcgtgg tcgctgcatc gggcaatccg ctcgcttatc ccatcatcag cgcgctccag
     1141 gaggagcggt tgatggccga tcccgacacc aagaaggtct acattcaggg gacggacggc
     1201 aagaacatcg atgccgcgac gggtgccgcc gtcgcttcca ttccggacag tgcgagcgct
     1261 gtgcgcctca acaaccgcct gcgccgcacc gtggaggcgg cgctcggcgg cctgaccctg
     1321 ttgtcgccgg atcccgccac ccgcatccag gctgcgcaat ccgtcttcaa atcgcatgag
     1381 gaggcggcgc tgccgacggt ggaaagcgcg cttgccaagg agaccgatgc aaacgcgaag
     1441 atagcgctca gcggggcgcg ggcggcgatc ctcctgttca agtccgacgc ctccgaagcc
     1501 gacaagctcg acgccgtcgc cacgatccgg gcgcgcggcg accaggaggc gatggcgctc
     1561 ctgacgggtc ttggcagcga acagccccca gcggtagcca aggccgcagc cgctgcgatt
     1621 tcctcgattg agagctcgct tgcgatatgg tcggcggtgc agaacgcatg gtacggcctg
     1681 tcgctcggct cggtgctcct gctcgcggcg atcgggctcg ccatcacctt cggcgtcatg
     1741 ggcgtcatca acatggcgca tggcgagatg gttatgctgg gcgcctatgc caccttcgtc
     1801 gtgcaggaag tcatccgcac ccgctatccg caggcgttcg attattcgct tttgatcgcg
     1861 gtaccgctcg ccttcctggt ctccggcgcc gtcggcatcc tgatcgaacg ctccatcatc
     1921 cgtttccttt acggccgtcc gctggaaacg ctgctcgcga cctggggcct gtcgctggtg
     1981 ttgcagcagg cggtgcgcac cgcattcggt ccgaccaacc gcgaagtcgg caatccctcc
     2041 tggatgagcg gcgcgttcga actcggccag atcaccatca cctataaccg gctctggatc
     2101 ctgtgcttta cgcttgccgt atttgccatc ctgctcgcca tgctgcgctt cactgcgctc
     2161 gggctcgaga tgcgcgcggt gacgcagaac cgccgcatgg cggcgtcgat gggaattgcc
     2221 acctcgcgcg tcgatgcgct gaccttcggg cttggctcgg ggattgccgg tattgccggc
     2281 gttgcgctgt cgcaaatcga caacgtgagc cccaatctgg gccagagcta tatcatcgac
     2341 tccttcatgg tggtcgtgtt cggcggcgtc ggcaatctct ggggcaccct ggttggcgcc
     2401 ttcacgcttg ggatcgccaa caagttcctc gagccggtcg cgggcgccgt tctcggcaag
     2461 atcgcaatcc tggtgctgat catcctgttt atccagaaac gcccgcgcgg cctgttcgcg
     2521 ctgaaggggc gagcggtgga agcatgatgc cgcatgtgct cacgcgttcg ctcgaccgtg
     2581 gcgcgacgat cttcatcctc gtggtcgcgg cctgcggaat cctgatcccg ctgtcgaacc
     2641 tgcttttgcc ggcgggctcg tttttccagg tgccgaccta tctggtggcg ctgtggggca
     2701 aatatgtctg ctacgccatt ctcgcgctct cgatcgacct gatctggggc tattgcggca
     2761 ttctctcgct cggccatggc gcctttttcg cgctcggcgg ctacgccatg ggcatgtacc
     2821 tgatgcgcca gatcggcacc cgcggcgttt acggcaatcc gatcctgccc gacttcatgg
     2881 tgtttctgaa ctatccgcgg ctgccctggt actggaacgg ctttgacatg ttctggttcg
     2941 ccgcgctgat ggtggtcctt gtcccgggac tgttggcctt ctgtttcggc tggtttgcgt
     3001 tccgctcccg cgtcaccggc gtctatctct cgatcatcac gcaggcgatg acctacgcgc
     3061 ttctgctcgg cttcttccgt aacgatttcg gctttggcgg caacaacgga ttgaccgatt
     3121 tcaaggacat tctcggcttc aacgtgcagg ccga
//
LOCUS       DATLJT010000817         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_52895,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000817 DATLJT010000000
VERSION     DATLJT010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52895"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VM910_18495"
     CDS             <1..474
                     /locus_tag="VM910_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64548.1"
                     /translation="TDTGARTAVSTESLPVVPYWHIWTDADGISHQSRCELGEFHKAP
                     IQPAAAPQWIGAETRNPATVFVTVLPVGWIGDWHENPRPQWIIPLSGRWFVESMDGVR
                     VEMGPGEISFGEDQNTRQVDGKRGHLSGTVGPEPAVLMLVQFEMMPMVASACRFR"
     gene            478..1383
                     /locus_tag="VM910_18500"
     CDS             478..1383
                     /locus_tag="VM910_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI64549.1"
                     /translation="MDGFDFRSPRFARYILSNAALETLATGFRWIEGPVWMGDANCLL
                     FQDLPNDRTMRWIEGQGVSVYRVPSHYANGQTRDRQGRLIACSHRGRCLYRTEYDGHV
                     TTLVDRHAGKRLNSPNDVVVKSDGSIWFTDPLYGISNDYEGGRQASEQPPAVYRLEPE
                     TGDIRIVADDFDGPNGLAFSPDETRLYIAETGNQTMPSPRQYIRVFNVGPDGATLSGG
                     DIFHKIEPGYCDGLRVDEYGNVWSSAGDGVHCVSAGGGLLGRIFVPHRVSNLTFGGLA
                     KNRLFIGGSTTLYAIFLNCRGVQVP"
     gene            1380..2318
                     /locus_tag="VM910_18505"
     CDS             1380..2318
                     /locus_tag="VM910_18505"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64550.1"
                     /translation="MTSVQKLSRITLICRQAERLAEFYTRAFGFACIEDTLKIDPGFA
                     ELIGLAKGQVRTTSLRLGNQVIALAEPQPAGCSYPGDVPGWDPLFQHFAIVVSDMTAA
                     YANLQALHNWTPISTDGPQILPSSSGGVTAFKFRDPEGHPLEMLAFAPGATPAHWAIR
                     SGSLCLGIDHSAISVADTSRSVAFYSRLGLARTASSLNVGREQEKLDNLLGAVVEVTA
                     LVPPMQAVPHVELLCYRGNFDRRELLTNRNDVAATQLVFEVERDALDGIVALNRDTTI
                     SRSTTSESGGLCALLRDPDGHLLCMEPRAPGPRALC"
     gene            2465..2701
                     /locus_tag="VM910_18510"
     CDS             2465..2701
                     /locus_tag="VM910_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64551.1"
                     /translation="MTTQESVARWKVLGFGPPPPIWSSRNIPCSWSTSPDRLVLGYAL
                     LAFGTAFEVIKGDPKLVVERCELVSKVVRLTTPI"
BASE COUNT          569 a          815 c          861 g          589 t
ORIGIN      
        1 actgacacag gggcacgtac ggccgtgagc acggaatcct tgcctgtcgt cccatactgg
       61 cacatctgga ccgatgccga tgggatcagc catcaatcgc ggtgcgagct gggcgagttt
      121 cacaaggctc cgatccagcc cgcagccgcc ccgcaatgga tcggtgccga gacgaggaat
      181 cccgcgacgg tgttcgtgac ggtgctgccg gttggctgga tcggagattg gcacgaaaat
      241 ccgcggccgc aatggatcat tcctttgtcc ggccgttggt tcgtcgagtc gatggatggc
      301 gttcgcgtcg aaatgggccc aggtgagatt tcgtttggcg aggatcagaa cacccgccaa
      361 gtcgacggta aacgggggca cctgtctggc acggtcggac cggaaccggc ggtcctgatg
      421 ctggtgcagt tcgagatgat gccgatggtc gcgtcggctt gccgcttccg gtgagccatg
      481 gatggttttg actttcgctc tccacgcttt gcgcgctata tcctgagcaa cgcggcgttg
      541 gagacactgg ccactggctt tcgctggatt gaggggccgg tgtggatggg cgatgccaac
      601 tgcttgctgt ttcaggacct acccaacgac cgcacgatgc gctggatcga agggcaaggt
      661 gtgtcggtgt accgcgtacc atcgcactac gcaaacggac aaacccgcga tcgccagggc
      721 cgcttgattg cctgctcgca ccgcggacgt tgtctctatc gaacagaata tgacggtcac
      781 gtcaccacat tagtcgatcg ccatgccggc aagagattga attcgccaaa cgacgtcgtg
      841 gtgaagtcag acggttcgat ctggttcacc gatccgctct acggcatctc gaacgactat
      901 gaaggaggac ggcaggcgtc ggaacagccg ccggcggtct atcggcttga accggaaacg
      961 ggcgacataa gaatcgtcgc ggacgatttc gacggcccca atgggctcgc tttttcgccc
     1021 gacgaaactc gactgtacat agctgaaacc ggaaaccaga cgatgccatc gccgcgccaa
     1081 tacattcgcg ttttcaatgt tggacccgat ggagcgacct tatccggcgg cgacatcttc
     1141 cacaaaatcg aaccgggata ctgcgatggt ttgcgggttg acgagtatgg caacgtctgg
     1201 agcagcgcag gcgatggtgt ccactgcgtc agtgcaggtg gcgggttgct cgggcggata
     1261 ttcgtgccac atcgggtctc caacctgacc ttcggaggcc tggcaaagaa tcgtcttttc
     1321 atcggcggct cgacaacatt atacgctatt ttcttgaatt gtcggggcgt gcaagtgccg
     1381 tgacctcggt tcagaagttg tcgcgaatca ccttaatctg ccgccaagcg gaacggttgg
     1441 cggagttcta tacacgcgcc ttcggcttcg cgtgtattga agacacgctg aagattgatc
     1501 cgggttttgc cgagctgatc ggacttgcaa aaggacaggt gcgaacaacg tccttgcgcc
     1561 tgggtaacca ggtaattgcg ctcgctgagc cgcagccagc aggatgcagc tatccgggcg
     1621 atgttcccgg atgggatccg ctgtttcagc atttcgccat tgtggtgtcc gatatgacag
     1681 cggcctatgc aaatttgcag gcccttcata actggacgcc aatctcaact gatggcccgc
     1741 aaattctgcc gtcatcctca ggcggggtga cggcgttcaa atttcgcgat cctgagggtc
     1801 atccgctcga aatgctcgcc tttgcacctg gcgcgacgcc tgcccattgg gcaatcaggt
     1861 ccggtagcct ctgtctgggc atcgatcatt ccgcaatttc cgtggccgat accagccgaa
     1921 gcgttgcgtt ctacagtcga ctggggctgg cgcgcactgc aagttccctc aacgtcggtc
     1981 gcgaacagga aaagctcgac aatcttctgg gagcggtcgt cgaggtgaca gctctggttc
     2041 ccccgatgca agctgtccct cacgtcgaac tgctatgcta tcgcggcaat ttcgatcgtc
     2101 gtgagctctt gaccaatcga aacgatgtgg cggcgacgca gctcgtgttc gaagtggaac
     2161 gcgacgcact tgatgggatt gttgcactta accgcgacac taccatctca aggtcgacca
     2221 catcggaaag cggaggatta tgcgccttat tacgggatcc ggacggtcac ttgctttgta
     2281 tggagccacg agcgcccggc ccgcgcgccc tgtgctaact ggcggagctg acagctcaac
     2341 acgctctatt gctgcaaacg acctcgacac gtcgtcacgg cccatcacgg ccgtctgccc
     2401 accagggctg cggaaagggt ccgatttctc acaacctgga tcggggcgac ttgaggagac
     2461 ccaaatgaca acacaggaaa gcgtggcaag atggaaggta ctcggcttcg gaccgccacc
     2521 gccgatatgg tcgagccgga acatcccgtg ctcttggagt acgtctcctg accggctcgt
     2581 gctcggctac gcgttgctcg cttttggcac ggccttcgag gtaattaagg gtgaccccaa
     2641 gctggtagta gagcgctgcg agttggtgtc taaggtcgtg cggctgacga ctccgatcta
     2701 gccgacagca agctgccccg cagagagaag gtgatgacga cgttccagtc gctgtaccgc
     2761 ctcgaccctc aggtgacggc atgagcgctg ccgagaacag agcgcctgcc cggcaggcgg
     2821 cgagggaacc ccat
//
LOCUS       DATLJT010000818         6336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1661415,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000818 DATLJT010000000
VERSION     DATLJT010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661415"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..763)
                     /gene="gstA"
                     /locus_tag="VM910_18515"
     CDS             complement(146..763)
                     /gene="gstA"
                     /locus_tag="VM910_18515"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HVI64552.1"
                     /translation="MKLYYAPGACSLFPHIALLEAGLPFDLVKVDLRAKKLENGDDFL
                     KVNPKGQVPALALDSGELVTEGPVVVQMIADRVKDKGLAPARDSADRYKLLEWLNYIT
                     TELHKNLGPLFSPVLTDDAKAFFKDRAMGKFKYLETQLAGRDYLMGKQFTVADGYLFT
                     MLMWATDRLNFDLSGLPNLLAYKARVAARPKVKEAMQKEGLLKAA"
     gene            899..1387
                     /locus_tag="VM910_18520"
     CDS             899..1387
                     /locus_tag="VM910_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI64553.1"
                     /translation="MKRRPSSEATAAWIRLMRVQSRVLDAVEQDLKKAGFPPLAWYDA
                     LLELSRAPSGELRPVELEKHMLIPQYSTSRLIDRLVDEGLAARRECKIDKRGQFVEIT
                     EAGRDLQKRMWSAYSSAIEKHVGSKLSDADAAKLCGLLDRLGCSCGDTASTAVGEGAS
                     AR"
     gene            1487..2857
                     /locus_tag="VM910_18525"
     CDS             1487..2857
                     /locus_tag="VM910_18525"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HVI64554.1"
                     /translation="MARDQIDMTPLKSRDELVAWLEAGVKPPSEFRIGTEHEKTPFTL
                     SGHRPVPYEGARGIGALLEGMKLLLGWEPIMERGNIIGLYDVTGGGAISLEPGGQFEL
                     SGAPVETVHQTQSELMAHLAQVREVAAPLGIGFLGLGMTPSWSRQEIPVMPKGRYNIM
                     TNYMPKVGRYGLDMMYRTCTVQTNLDFSSEKDMVKKLRVSLALQPVATALFANSPFTE
                     GKPNGFLSFRSEIWRDTDNARAGMLSWVFDEGMGFERWVDYALDVPMYFVKRGESYID
                     VAGSSFRAFFDGRNNSLPGERPTLSDWANHLSTIFPEVRLKRYLEMRGADGGPWSRLP
                     ALPAFWVGLLYDDTSLDAAWDVAKRWNAHERQALRDDVPRLGFKARIGNRYLFEVAKE
                     CLALAHAGLRRRGRIDHAGRDETRHLEPLDRIIDCGRTPAEEMLEKFNGPWRGSVEPA
                     YVEYAF"
     gene            3010..3552
                     /locus_tag="VM910_18530"
     CDS             3010..3552
                     /locus_tag="VM910_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HVI64555.1"
                     /translation="MGIRLIGAWLVTLACLAAPLSAWAQTNFDRPGGDYMSSPVPSGD
                     PADCALACERDRRCRAWSFNYPTDPAIGAVCWLKNTVPARVQDNCCVSGVRGAGVVEP
                     RSAALETSIDRPGGDYKSFEIKMGEAEENCQAACAADNKCRAWTFARAGYVGKDAHCY
                     LKKDIKPPRRKAGYISGVVR"
     gene            complement(3585..4367)
                     /gene="lepB"
                     /locus_tag="VM910_18535"
     CDS             complement(3585..4367)
                     /gene="lepB"
                     /locus_tag="VM910_18535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVI64556.1"
                     /translation="MSLEKLVSANGGTKSSSNWRGQAMQLVAIVLAVFLAKGAIAEPF
                     YVPSGSMEPTLLIGDALLASKFPYGYGTASLPMQINLPETSRVFQEMPKRGDVVVFRW
                     PGDTSQAWVKRVVGVPGDRIQMRQGQLFINDHPATLKPDGTGDAEDDNGNVERAWRYV
                     ETLPNGVSHAIYKLRDNGRLDNTVEVTVPPGKLFVLGDNRDNSADSRVPVREGGVGLL
                     PIDNLVGRADAVVGSWDLGMRSQPVWTWLSGFRLARFFTAVH"
     gene            complement(4364..5791)
                     /gene="tldD"
                     /locus_tag="VM910_18540"
     CDS             complement(4364..5791)
                     /gene="tldD"
                     /locus_tag="VM910_18540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HVI64557.1"
                     /translation="MTDPATTSLLDRANLDRDAVRREVGRGLNGADDGELFLEYSQSE
                     ALTFDNGRLKQATYDTSQGFGLRAVKDDAVGYAHSSDVSLPALIRAADAVLAVRGGYS
                     GSFAAPPPHTNVRLYRDDNPLDAPGFESKVKLLAEIDAYLRDKDPRVRQVTVSLGATW
                     QVVEILRPDGESYRDIRPLVRVNISVVAGQGDRQETGSKGYGGREGYARFIETKSWRE
                     AADGALREALVNLESVPAPAGEMDVVLGSGWPGVMLHEAVGHGLEGDFNRKKTSAFAG
                     LMGRQVAAKGVTVVDDGTMASRRGSLSIDDEGTPTNRTVLIEDGILVGYMQDRQNARL
                     MQMKPTGNGRRQGYAHVPMPRMTNTYMLAGGRDPAEIIASVKRGIYAANFGGGQVDIT
                     SGKYVFQCTEAYKIENGKLGAPLKGAMLIGNGPTDLNRITMIGNDLALDTGIGTCGKN
                     GQGVPVGVGQPTLRMEGITVGGTGR"
     gene            complement(5955..>6336)
                     /locus_tag="VM910_18545"
     CDS             complement(5955..>6336)
                     /locus_tag="VM910_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI64558.1"
                     /translation="TPPGAQNEQCALIQSVVAEDRSNAGLTVIVLKTADQKNRLMRVV
                     APLGVLLPSGLGLKLDNVDVGRAGFVRCLPNGCVAEVIMDDKLLGQLKSAKTATFIIF
                     ETPEEGIGFPLSLNGLSDGFDKLP"
BASE COUNT         1114 a         1985 c         2056 g         1181 t
ORIGIN      
        1 atcaggcgcg gttgatgcga ggacagacca agcggcacga aaatcaaccc atgggcaccg
       61 tcgtgcggat tgatgcgagc ggcgagtttg gctttctcac cgcgatctgt cctccttttc
      121 aaggagaggt tggagagaag gcgccttacg ccgccttcag caagccttcc ttctgcatcg
      181 cctccttcac cttcggtcgc gcggcgacgc gggccttgta ggcgagcagg ttcggcagtc
      241 ccgacaaatc gaagttcagc cggtcggtgg cccacattag catcgtgaac agatagccgt
      301 cggcgaccgt gaactgcttg cccatcaggt agtcgcgacc ggcaagctgg gtctccagat
      361 atttgaattt gcccatggcg cggtccttga aaaaggcctt ggcgtcatcg gtcaggaccg
      421 gcgagaacag cgggcccaga ttcttgtgca gctcggtggt gatgtagttc agccattcga
      481 gcagcttgta gcggtcggcc gagtcgcgcg caggcgctag ccccttgtct ttgacgcggt
      541 cggcgatcat ctgcacgacc acgggccctt cggtgacgag ctcgccggaa tccagtgcga
      601 gcgccggcac ctggcccttc gggttgactt tcaaaaagtc atcgccgttt tccagtttct
      661 tggccctcag atcgaccttg accaggtcaa agggaaggcc ggcttccagg agcgcgatgt
      721 gcgggaacag cgagcaggcg ccgggggcgt agtagagctt catcgggaat ttcctcctgt
      781 gacatcttgg gtcaggcgga caaatgccta aatgcaattg catacaatag tcaagattga
      841 tgcagatgca tcgagtgggc tacacttgag cgctggtagg gcatcaatgg gctaaccgat
      901 gaagcgtaga ccgtcgagcg aggcgaccgc cgcctggatc cgtctgatgc gggtgcagag
      961 ccgggtgctg gacgccgtcg agcaggatct gaagaaggcc ggctttccgc cgctcgcctg
     1021 gtatgacgcg ctgctggagc tgtcgcgcgc gccctccggc gaactgcggc cggtggaact
     1081 ggaaaagcac atgctgattc cgcaatattc cacctcgcgg ctgatcgacc ggctggtcga
     1141 cgaagggctc gcggcacggc gcgagtgcaa gatcgacaag cgcggccagt tcgtcgagat
     1201 caccgaagcc gggcgcgacc tgcagaagcg gatgtggagc gcctattcgt cggcgatcga
     1261 aaagcatgtc ggctcgaaac tgtcggatgc ggacgctgcc aagctctgcg gcctgcttga
     1321 ccggctcggc tgctcgtgcg gtgacacggc ctcaaccgcc gtgggcgagg gtgcgtcggc
     1381 ccgatgacct atcatccccc tgttgcgtct gcggcgcctc tggtttccgc ggggcgcgac
     1441 cccgacaccc tgcgcccggg acaggcgcct ttgatccgga ttttgcatgg cgcgtgacca
     1501 aatcgatatg acgccgctga aatcgcgtga cgaactcgtc gcgtggctgg aagcgggcgt
     1561 gaagccgcca tccgaattcc ggatcggcac cgagcatgag aagacgccgt ttacattgtc
     1621 gggacatcga cccgtaccct acgagggcgc gcgcggcatc ggggcgttgc tcgaaggcat
     1681 gaagctcctg ctcggctggg agccgatcat ggagcggggc aatatcatcg gcctctacga
     1741 cgtcaccggc ggcggggcga tctcgctcga gccgggcggc cagttcgaat tgtcgggcgc
     1801 gccggtcgaa accgtgcacc agacgcaatc ggaattgatg gcgcatctcg cccaggtgcg
     1861 cgaggtggcg gctcccctgg ggatcggctt cctcgggctt ggcatgacgc cgtcctggtc
     1921 gcgccaggag attccggtga tgcccaaggg ccgctacaac atcatgacca attacatgcc
     1981 gaaggtcggc cgctacggcc tcgatatgat gtaccggacc tgcacggtgc agaccaatct
     2041 cgacttctcc tccgaaaaag acatggtcaa gaagcttcgc gtctcgctgg cgctacagcc
     2101 cgtcgccacc gcgctgttcg ccaattcgcc cttcaccgag ggcaagccca acggatttct
     2161 atcgttccgt tccgagatct ggcgcgacac cgacaatgcg cgcgcaggca tgctgtcatg
     2221 ggtgttcgac gaaggcatgg gattcgagcg ctgggtcgat tacgcgctcg atgtcccgat
     2281 gtattttgtg aagcgcggcg agagctacat cgatgttgcc ggctcctcgt ttcgggcctt
     2341 cttcgatggc cgcaacaatt ccctgcccgg cgagcgtccg accttgtcgg attgggccaa
     2401 tcatctgtcc acgatcttcc cggaggtgcg tctgaagcgg tatctggaaa tgcgtggcgc
     2461 ggatggcggg ccgtggagcc gcttgccggc tctgcctgcg ttttgggtcg ggctgctcta
     2521 tgacgacacc agccttgacg ccgcctggga cgtcgccaag cgctggaacg cgcatgagcg
     2581 ccaggcgctg cgcgacgatg tcccgcggct cggcttcaag gcgaggatcg gtaaccgcta
     2641 tttgtttgaa gtcgccaagg aatgcctggc gctggcgcat gctggcctgc gccgccgtgg
     2701 ccggatcgac catgccgggc gcgacgagac gcggcatctt gagccgctgg atcgcatcat
     2761 cgattgcggg cgcacgccgg ccgaggagat gctggagaaa ttcaacggtc cctggcgcgg
     2821 ctcggttgaa ccggcctatg tggaatatgc cttctaggag catgatccgg aaaggcattc
     2881 cccgcacgtg atgcggggcg ggcgccggta acgtttttcc gccgttcatg cgccgtacag
     2941 gttcatggcg atcaaatggc ggcgtcgaaa acctgaaaac gcgatcgaat cgcgcgaaag
     3001 cactgccgca tggggatccg tcttattggg gcgtggttgg tgaccctcgc ctgtctggcg
     3061 gcgcccctat ccgcatgggc acagaccaac ttcgaccgtc ccgggggcga ctatatgagt
     3121 tcgcccgttc cctccggcga cccggccgat tgcgcgcttg cctgcgagcg tgaccgccgc
     3181 tgtcgggcgt ggagcttcaa ctatccgacc gaccccgcga tcggcgccgt gtgctggctg
     3241 aagaacaccg tgccggcgcg cgtacaggac aattgctgtg tctccggtgt gcgcggtgcc
     3301 ggcgtggtcg aaccgcgaag cgccgcgctg gagacctcga tcgaccggcc gggcggcgac
     3361 tacaagagct ttgaaatcaa gatgggcgag gccgaggaga attgccaggc agcctgcgct
     3421 gccgacaaca aatgccgggc ctggaccttt gcgcgcgccg gttatgtcgg caaggacgcg
     3481 cactgctatc tgaaaaaaga catcaagccg ccgcggcgga aggcgggata tatctccggc
     3541 gtcgtgcgtt aggcgcgaaa catccgaagg tcgtcatctt ccgttcaatg cacggcggtg
     3601 aagaaccgtg cgaggcgaaa gccggacagc catgtccata ccggctggct gcgcatgccg
     3661 agatcccagg agcccaccac cgcgtccgcg cggccgacca gattgtcgat cggcagcagc
     3721 ccgacgccgc cttcgcgaac cgggacgcgg ctgtcggcgg aattgtcgcg attatcgccg
     3781 agtacgaaga gttttcccgg cggcacggtc acttccaccg tgttatcgag ccggccgttg
     3841 tcgcgcagct tgtagatcgc atgagaaacg ccgttcggca gcgtctcgac atagcgccag
     3901 gcgcgctcga cattgccgtt atcgtcctcc gcatcacccg tgccgtcggg tttgagcgtc
     3961 gccgggtgat cgttgatgaa gagctggccc tggcgcatct ggatacggtc gccgggcacg
     4021 cctacgacgc gcttgaccca ggcctgcgag gtgtcgcccg gccagcggaa caccacgacg
     4081 tcgccgcgct tcggcatctc ctggaacacc cgacttgtct ccggcaggtt gatctgcatc
     4141 ggcagcgagg ctgtgccgta gccgtagggg aatttcgacg cgagcagcgc gtcgccgatc
     4201 aaaagcgtcg gttccatcga gcccgagggc acgtaaaagg gttccgcaat cgcgcccttg
     4261 gcgaggaaga cggccagcac gatagcgaca agctgcatgg cctgtccgcg ccagttgctg
     4321 ctgcttttgg tgccgccatt ggcgctgacg agtttctcca ggctcatcgg ccggttcctc
     4381 cgacggtaat gccttccata cgcagcgtcg gctggccgac gccgaccggc acgccctggc
     4441 cgttcttgcc gcaggtaccg atgccggtat cgagcgcgag gtcgttgccg atcatggtga
     4501 tgcggttgag atcggtcggc ccgttgccga tcagcatcgc gcccttgagc ggcgcgccca
     4561 gtttgccgtt ctcgatcttg taggcctcgg tgcactggaa cacatatttg cccgaggtga
     4621 tgtcgacctg gccgccgccg aaattcgcgg cataaattcc tctcttcacg gatgcgatga
     4681 tctccgcggg atcgcggcct ccggcgagca tgtaggtgtt ggtcatgcgc ggcatcggca
     4741 cgtgggcata gccctggcgg cggccgttgc cggtcggctt catctgcatc agccgcgcgt
     4801 tctggcggtc ctgcatgtag ccgaccagaa tgccgtcctc gatcagcacg gtgcggttgg
     4861 tcggcgtgcc ctcgtcgtcg atcgacagcg aaccgcgtcg cgaggccatg gtgccgtcat
     4921 cgaccacggt gacgcccttt gctgcgacct gccgacccat caggcctgcg aaggccgagg
     4981 tcttcttgcg gttgaagtcg ccttcgagcc catgaccgac ggcttcatgc agcatcacgc
     5041 cgggccagcc cgagccgagc acgacgtcca tttcgccggc gggagccggc accgattcca
     5101 gattgaccag cgcctcgcgc agcgcgccgt cggccgcctc gcgccaggat ttcgtttcga
     5161 tgaagcgcgc atagccttcg cggccgccat agcccttcga gccggtctcc tgcctgtcgc
     5221 cctgaccagc caccacggaa atattgactc gcaccagcgg gcggatgtcg cggtagcttt
     5281 cgccgtcagg ccgcaagatt tccaccacct gccaggtggc gcccaggctc accgtcactt
     5341 gccgcacccg cggatccttg tcgcgcaaat aggcgtcgat ctcggcgagc agtttcactt
     5401 tgctctcgaa accgggtgca tcaagcggat tgtcgtcgcg atagagccgg acgttggtgt
     5461 gcggcggggg agcggcgaaa gagccggaat agccgccgcg cacggcgagc acggcgtccg
     5521 ccgcgcggat caaggccggc agcgagacat cggaagaatg cgcatagccg accgcgtcgt
     5581 ccttcaccgc ccgcaggccg aaaccctggc tggtgtcgta ggtcgcctgt ttcaggcgcc
     5641 cattatcgaa ggtcaacgct tcgctctgac tatattccag gaacaactcg ccatcgtcgg
     5701 cgccgtttag cccgcggccg acttcgcggc ggacggcatc gcgatcgaga ttggcgcgat
     5761 cgagaagcga ggttgtggcg ggatcggtca tgaagactcc aacgcaaatg ccgagggccg
     5821 ggccactcta tcgactgcaa gcggggcggt cgtcttacag agtggtaacc tcgtcctcgg
     5881 ctcgtccagc aaagatagtc acgaaagcac gcaaaaggga gagccggcgc agcagtttgc
     5941 cgcgccggtt cctgtcacgg cagcttgtca aatccgtccg agagcccgtt caggctcaag
     6001 gggaagccga tgccctcttc cggcgtctcg aagatgatga aggtcgcagt cttggcggac
     6061 ttcaactgtc cgagcagctt gtcgtccatg atcacctcgg cgacacagcc atttggcagg
     6121 cagcgaacga aaccggcgcg gccgacatcg acattgtcga gtttcaagcc caacccggat
     6181 ggcagcagca cgccgagcgg cgccacaacc cgcatcaggc ggttcttctg gtcggcggtc
     6241 ttgagcacga tcaccgtcag tccggcattc gagcggtcct cggcgaccac gctctggatc
     6301 agggcgcatt gctcgttctg ggcaccgggc ggggtg
//
LOCUS       DATLJT010000819         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3499574,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000819 DATLJT010000000
VERSION     DATLJT010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499574"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(525..1400)
                     /gene="prmC"
                     /locus_tag="VM910_18550"
     CDS             complement(525..1400)
                     /gene="prmC"
                     /locus_tag="VM910_18550"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVI64559.1"
                     /translation="MNTFAGQSVEAARRALAARLKASCIESAELEARTLVGAALGLDL
                     TGLVASATRRLGSAEADRLDDFTRRRLAGEPVARILGKKEFWGVSFKLSEATLVPRPD
                     TETVVEQALEILRASPISAVRIADIGTGSGAILLALLLELPQAFGIGTDLSEGALGIA
                     QENASQLGLGGRAAFVACNYAAALSGGFDLIVSNPPYVRSADIARLAVEVRVHDPLLA
                     LDGGTDGLEAYRTLIPEAAGLLKPGGALVVEAGRGQADDVGALMAAAGLMAPWAPKTD
                     LAGIPRAVTGRKMLV"
     gene            complement(1397..2482)
                     /gene="prfA"
                     /locus_tag="VM910_18555"
     CDS             complement(1397..2482)
                     /gene="prfA"
                     /locus_tag="VM910_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVI64560.1"
                     /translation="MSLLPETKLDVLLAHHASLEAQLLGQLASEKYVQITRELAELNP
                     LIDAVKAYRATAKELGDTEALVTDPASDPEMRAMAEVERDTLLARRDELVQKIRVALL
                     PKDAMDERNVMLEIRAGTGGDEAALFAGDLFRMYERFASLQGWAVDVISASDGTMGGY
                     KEIVAEVKGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEVEDVDVEIR
                     NEDLRIETMRAQGAGGQHVNKTESAIRITHIPTGIVVSMQDSRSQHKNRASAMNILRS
                     RIYDAERQRVEAARSADRKEKVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLPQVMS
                     GEALGELIDALTTEHQAAQLAAQGAAA"
     gene            complement(2536..>2962)
                     /locus_tag="VM910_18560"
     CDS             complement(2536..>2962)
                     /locus_tag="VM910_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVI64561.1"
                     /translation="YQLDELLGKVDFVSVGSNDLFQFLFAVDRGNAKVSERFDTLSAP
                     ILRALRDIVRKAKAKNKSASLCGEMASKPIGALALIALGYRSLSLSATGHGPVKAMIL
                     ELNAKKAEAVMTPLLDAPAGSVSIRQKLIEFAEAEGLPL"
BASE COUNT          508 a          957 c          909 g          588 t
ORIGIN      
        1 ggccgcgccg gttgttattg ttgttgttat tgttccggtt gcgcatccgc ttgttatttt
       61 gaccgtttct catgtcctgc ctttatttcc agccctgagt taaagcactg aattgccggc
      121 gcgaccgaaa ctggcaagcg cgcgggtgca agcgcacgga aacccgctgc cgtctcgatc
      181 cctgccgatt tcgtcaaccg tcgtcttcaa caaagacgcg ttccccaccc gccggcgccc
      241 cggcgaacct ttcgttcagc ctgtcaaaac aaaccctgtt ttcttgagta ggtcttcaag
      301 cgcaaaatca ggctttcgtt cgatttgcgg tcggaagcgg cgcagcttga cttaagccct
      361 cgcgctcaat tcgacccgcg tttcggaacc tttcacccgg cgtggctctg gcttgagaac
      421 cctggatttg gcccgcttcg tctcgggacc tgcgccggtg ttgtggcggg aaagtagtcg
      481 gtcccaagga atattccaag aggtttttgc gttccacagg ggtttcaaac cagcattttt
      541 cggcctgtta cggcccgggg aatacccgcc agatcggttt tcggggccca cggggccatc
      601 aaccccgccg ccgccatcaa ggctccaacg tcgtccgcct ggcctcggcc ggcctccacc
      661 accagagccc ctccgggctt caacaggcct gcggcctcgg gaatcaaggt acggtacgcc
      721 tcaagcccgt cggttccgcc atcgagcgcc agaagcgggt cgtgcacgcg gacttcaacg
      781 gcaaggcgcg cgatatccgc ggagcggaca taaggcgggt tcgacacgat gaggtcgaaa
      841 ccgcccgaca acgctgcggc atagttgcac gccacgaagg ccgcacgtcc gccaagtccg
      901 agttgactgg cgttttcctg cgcgatccca agagcgcctt cgctgagatc ggtgccgatg
      961 ccgaacgcct gcggcagttc cagcagcagc gccaacagaa tcgcacccga gccggtgccg
     1021 atatcggcga ttctcaccgc tgatatgggg ctcgcgcgca ggatttccag ggcctgttcg
     1081 accacggttt cggtgtccgg ccgcggcacc agtgtcgctt ccgacagttt gaacgaaacg
     1141 ccccaaaatt cctttttgcc gagaatgcgc gcgaccggtt cgccggcaag gcgccgccgc
     1201 gtgaaatcgt ctagccgatc ggcctcggcc gaaccgagcc gtcgtgtcgc ggacgcgacc
     1261 agaccggtga ggtcaaggcc gagcgccgca cccacaagcg tccgcgcctc gagctctgcc
     1321 gattcgatgc aggacgcttt gagccgtgcc gcgagcgcgc gacgcgcagc ttcgacggat
     1381 tgccccgcga atgtgttcac gccgccgcgc cctgcgcagc gagctgcgcc gcctgatgct
     1441 cggtcgtgag cgcatcgatc agttcgccga gtgcttcgcc ggacatcacc tgcggcagct
     1501 tgtagagcgt caggttgatg cggtggtcgg tgacgcgtcc ttgcgggaaa ttataggtgc
     1561 ggatgcgctc gctgcggtcg ccggagccga ccttctcctt gcggtcggcc gagcgcgccg
     1621 cttccacgcg ctggcgctcg gcgtcgtaga tgcgcgagcg caggatgttc atggcggatg
     1681 cgcggttttt gtgttgcgag cggctgtcct gcatgctgac cacgatcccg gtcgggatat
     1741 gggtgatacg gatcgccgat tccgtcttgt tgacatgttg gccgccggcg ccttgtgcgc
     1801 gcatcgtctc gatcctcaag tcttcgttcc tgatctcgac atcgacgtcc tcgacctccg
     1861 gcagcacggc cacggtcgcg gccgaggtgt gaatgcgtcc ctgggtttcg gtgtcgggca
     1921 cgcgctgcac gcgatgcacc ccggattcga atttcagttt tgcaaaagca ccgcggccct
     1981 tcacctcggc gacgatttcc ttgtaaccgc ccatggtgcc gtcggaggcc gagattacgt
     2041 cgacggccca gccctgcaga ctggcgaaac gctcgtacat ccgaaacagg tcgccggcaa
     2101 acagcgcggc ctcgtcgccg ccggtcccgg cgcggatttc cagcatcacg ttgcgctcat
     2161 ccatggcgtc ctttggcaac agggcgacgc ggatcttctg tacgagctca tcgcggcggg
     2221 cgaggagtgt gtcgcgctcg acttccgcca tggcacgcat ctcgggatcg gatgcgggat
     2281 cggtgaccag cgcttcggta tcgccgagtt ctttcgcggt cgcacgataa gccttgaccg
     2341 catcgatcag cggattgagc tcggcgagct cgcgggtgat ctgcacgtat ttctcggaag
     2401 cgagttggcc gagcaactgc gcctccagcg aggcgtggtg cgccagcaga acgtccagtt
     2461 tggtttcagg aagcagcgac atcggtcaaa tctcaaagca acggaaaagg gcggctgggg
     2521 cggcgcgaag cgtcgctaca acggcaatcc ttcggcttcc gcaaattcga tcagcttctg
     2581 ccggatcgag acactgcctg ccggcgcgtc gagaagcggg gtcatcaccg cttccgcctt
     2641 cttcgcgttt agctccagga tcatcgcctt caccggacca tgcccggttg cggacaatga
     2701 cagagaacga tagccgagcg cgatcaatgc cagcgcgccg atcggctttg aggccatctc
     2761 gccgcacagc gaggcagatt tgttcttggc tttcgccttc cgcacgatgt cgcgcaacgc
     2821 gcgcaggatc ggcgccgaca gcgtatcgaa ccgctccgaa accttggcgt tgccgcggtc
     2881 gaccgcgaac aggaactgga acaggtcgtt cgaacccacc gagacgaagt cgaccttccc
     2941 caggagctcg tcgagttgat ag
//
LOCUS       DATLJT010000820         3943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2120189,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000820 DATLJT010000000
VERSION     DATLJT010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120189"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VM910_18565"
     CDS             <1..692
                     /locus_tag="VM910_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64562.1"
                     /translation="NIPHAFAVQSMVGELAHATNRDQKDMLLELIGSPRIVNLSSVKD
                     LWNYGEPYDSYPIDTARLRRVVELVAEKAGWGRSVPKGHGLGIAVHRSFVSYIATVVE
                     VSVDDKGKLTVPRVDTAIDCGTYINPERIRSQIEGAAIMGLSLTKYGEVTFKDGKVQQ
                     GNFDDFPLVRIDEAPLVTNVYIVPPAADTPPSGAGEPGVPPFAPALINAIFAATGKRI
                     RSLPIGKQLET"
     gene            869..1543
                     /locus_tag="VM910_18570"
     CDS             869..1543
                     /locus_tag="VM910_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HVI64563.1"
                     /translation="MIGFQICKRARKVDPGITAKFRELPVANISDSMSRMAAGGPSLR
                     PIHKAGPLAGPAFTVKTRPGDNLMVHKAIDIADPGDVIVVDGGGDLTNSLIGELMSAY
                     AESRGIAGIVIYGAIRDYDTLHAGSFPIYAAGVTHRGPYKDGPGEINVPIAIAGMVIE
                     PGDLIVGDGDGIVCVPYDLAAEVLKATETKHQAEIKQLAAIKQGKNDRAWVDAALTRL
                     GCKIHD"
     gene            1570..2754
                     /locus_tag="VM910_18575"
     CDS             1570..2754
                     /locus_tag="VM910_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64564.1"
                     /translation="MLTFSRTKHFAGCACCGAGAMTFPAIAAAATSSSLPPPVNVEAI
                     AAKQAASSSAATIIDCHGHYTTEPEALLDWRKRQIAAIGDRSQMPTVDQLKVNDDELR
                     ISVEGAQLKLQRERGTSVTLFSPRASGMSHHIGDAAVSAAWSHVSNDLIYRITKLYPK
                     NFIGVCQLPQSPGVAPKNCIEELTRCVQQLGFVGCNLNPDPSGGYWTDPPLTDRWWYP
                     LYEKMVELDVPAMIHVSAAANPVFHTTASHYINGDTTAFVQFLMSDLFKDFPTLKLII
                     PHGGGAIPYHWGRYRGIAQDQKRPPLSESMLKNVFFDTCVYHQPGIDLLTRVIPAENI
                     LFASEMVGAVRGVDPETGFNYDDTRRYIDGSSTLTSEQKRAIFAGNARRVYSRIPADI
                     FH"
     gene            complement(2807..3190)
                     /locus_tag="VM910_18580"
     CDS             complement(2807..3190)
                     /locus_tag="VM910_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI64565.1"
                     /translation="MSKSELFEKGLKVRKEVLGEDYVNKSIAGADEFTRTMAEWSTEF
                     CWGALWTRPGLDRRTRSIVNLAMLAALNRPHELKLHVKGALKNGLTRDEIKEILLQVA
                     VYCGVPAGIDAFRNAREAFKELEGQ"
     gene            3455..>3943
                     /locus_tag="VM910_18585"
     CDS             3455..>3943
                     /locus_tag="VM910_18585"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI64566.1"
                     /translation="MNIESKVADDSAHDLIEIVPTGRALGAEIRGVDLTSLDDLHFAA
                     LKRAWHQHQVILVRGQTLRDQDLIAFSRRFGDLDWAPVQETGRRFVEGLPEIYIVSNV
                     KVNGEAIGSLGAGEAVWHTDMSYLDVPPMASMLYAIEVPPVGGNTSFCSMYAIYEALP
                     PEI"
BASE COUNT          743 a         1279 c         1166 g          755 t
ORIGIN      
        1 cgaacattcc gcatgccttc gccgtgcagt cgatggtcgg cgaactcgcg cacgcgacca
       61 atcgcgatca gaaggacatg ttgctggagc tgattggctc gccccggatc gtcaacctgt
      121 cttccgtaaa ggacctgtgg aactacggcg agccctatga cagctatccg atcgacacgg
      181 cacgccttcg tcgcgtggtc gagttggtcg cggagaaggc cggttggggc cggtcggtgc
      241 cgaaaggaca cgggctcggg atcgcggttc accgcagctt cgtcagctat atcgcgaccg
      301 tcgtcgaagt atcggtcgac gacaagggca agctgacggt cccccgggtg gataccgcga
      361 tcgattgcgg gacctacatc aaccccgaac gcattcgctc gcaaatcgag ggcgcggcga
      421 tcatgggctt gagccttacc aaatatgggg aggtcacctt caaggacggc aaggtccagc
      481 aaggcaattt cgatgatttc ccgctggttc ggatcgacga ggctccgctc gtgaccaacg
      541 tgtacatcgt gcctcccgct gccgacaccc cgccctcggg cgccggcgaa cccggcgtcc
      601 cgccctttgc gccggcattg atcaacgcga tcttcgcggc aacgggaaaa cgtatccggt
      661 cgcttccgat cggcaaacaa ttggagacgt gaagccgtaa gcgtcgggag atcgccaagc
      721 ctcatcgccg aggcacaagg acgcccgcag cagggatgct gcgggcgtcc ctttttcgat
      781 gtgccgaccg ccgacgccga caaggcgggt gacttgtctt gccgcgaaga atgtaatttc
      841 atcataccca agacaaggga ggtttgcggt gatcggattc cagatttgca agcgcgcgcg
      901 caaggtcgac cccggcatca cggcgaagtt tcgcgagctt cccgtcgcca atatcagcga
      961 ctccatgtcg cgcatggccg ccggcgggcc atcgcttcgg ccgatccaca aggcaggccc
     1021 gcttgccggc cccgccttta cggtcaagac caggcccggc gacaatctca tggtgcacaa
     1081 ggcaatcgat atcgccgatc ccggcgacgt cattgtggtc gatggcggcg gcgatctcac
     1141 caactcgctg atcggcgagt tgatgtcggc atatgcggag agccgcggca tcgccggcat
     1201 agtcatttac ggtgcgatcc gcgactacga cacgctccat gccggtagtt tcccgattta
     1261 tgctgccggg gtgacccatc gcgggccgta caaggacggc cccggcgaga tcaatgtgcc
     1321 gatcgcgatc gcaggcatgg tcatcgagcc gggcgacctg atcgtcggcg acggcgacgg
     1381 catcgtctgc gttccctacg atctggctgc cgaggtgctg aaggccaccg aaaccaagca
     1441 tcaggccgaa atcaaacagc ttgccgcaat caagcaaggc aagaacgacc gtgcctgggt
     1501 cgatgccgcc ctgacgaggc tcggctgcaa gattcacgac tgaccgcgct ttcatcgagg
     1561 agtaacagcg tgctgacttt ctccaggaca aagcacttcg ccggctgcgc gtgttgcggt
     1621 gccggcgcca tgacctttcc ggcgattgcg gccgccgcga cgtcttcatc gcttccgcct
     1681 cccgtgaacg tcgaagcgat cgccgccaaa caggcggcct cgtcgtcggc agctaccatc
     1741 atcgactgcc acggacatta caccaccgag cccgaggcgc tgctcgattg gcgcaaacgg
     1801 cagattgcgg cgatcggcga ccgctcgcag atgccgacgg tcgatcagct caaggtgaac
     1861 gacgatgagc tgcgcatctc ggtcgagggt gcccagctca agctgcagcg cgagcgcggc
     1921 acgtcggtga cgctgttctc cccgcgcgcc tcaggcatga gccatcacat cggcgacgcc
     1981 gccgtcagcg cggcatggtc gcatgtatcc aacgacctga tctacaggat cacgaaactc
     2041 tatccgaaga atttcatcgg cgtctgccag ctcccgcaat cccccggcgt cgcgcccaag
     2101 aactgcatcg aggagctgac gcgctgcgtc caacagctcg gttttgtcgg ctgcaacctc
     2161 aaccccgatc cttccggcgg ctattggacc gatccgccgc tgaccgatcg ctggtggtat
     2221 ccgctctatg agaagatggt tgagctcgat gtgcccgcga tgatccacgt cagcgcagcc
     2281 gccaacccgg tcttccacac cacggccagt cattacatca atggcgacac cacggccttc
     2341 gtgcagttcc tgatgtccga cctgttcaag gattttccga ccttgaaact gatcattccg
     2401 cacggcggcg gcgccattcc ctatcactgg ggccgctacc gcggcattgc gcaagaccag
     2461 aagcggccgc cgctgtccga atccatgctc aagaacgtgt tcttcgacac ctgcgtgtat
     2521 caccagccgg gtatcgatct cctcacccgc gtgatccccg ccgaaaacat tctgtttgcc
     2581 tccgaaatgg tcggcgcggt gcgaggcgtc gaccctgaaa ccggcttcaa ctatgacgat
     2641 acccgtcgct acatcgatgg ctcgtcgacg ctgacatccg agcagaagcg cgcgattttt
     2701 gccggcaatg cgaggcgcgt ctatagccgc atccccgccg acatcttcca ctgagccgcc
     2761 gtgcggtcaa tcgagaacgc cgacgtctct cttcgctgcg cgagccttac tggccctcca
     2821 gctccttgaa ggcctcgcgc gcgttgcgga aggcgtcgat ccccgccggc acgccgcaat
     2881 agaccgcgac ctgcagcagg atttccttga tctcgtccct ggtcaggccg ttcttcagcg
     2941 cgcccttgac atgcagcttc aattcgtggg gcctgttgag cgcggcgagc atcgcaagat
     3001 tgacgatgct ccgggttcgc cgatcgagcc ccggccgcgt ccacaatgcg ccccagcaga
     3061 actccgtcga ccattcggcc attgtacgtg tgaattcatc ggcgccggca atggacttgt
     3121 tgacgtaatc ctccccgagc acctccttgc ggaccttgag gcccttctcg aacagttcgc
     3181 tcttgctcat gattgtcctt ccttgctggc gggcgaattc tctcaaaatt gttgtttcaa
     3241 tcctatgccg ggcggcgacg gactgtcgac ggcaaagcat cggcacagat caggccggtg
     3301 catgcctgtg atgcggcgca agcattcccg agagacgcgc cgccgcgccc aagcgggctc
     3361 cgccatcgtc tttattactg tgtcggatga taatattgtc ccggacgatc ctcgaggtaa
     3421 tcgcttgacc ggtgctgcca gggaggccga ctccatgaat atcgaaagca aggttgccga
     3481 cgactcggcg catgacttga tcgaaatcgt tccgaccggc cgcgcacttg gcgccgagat
     3541 ccggggcgtc gacctgacat ctctcgacga tctgcacttt gcggcgctca aacgcgcctg
     3601 gcaccagcat caggtgatcc tggtccgcgg ccagaccctg cgcgatcagg atctcatcgc
     3661 attcagccgc cgctttggcg atctcgattg ggcgccggtg caggaaactg gccggcgttt
     3721 tgtcgaaggc ctgccggaga tttatatcgt gtcgaacgtg aaggtgaacg gcgaggcgat
     3781 cggcagcctg ggcgccggcg aagccgtatg gcacaccgac atgtcctatc ttgatgtgcc
     3841 gccgatggcg agcatgcttt atgcgatcga ggtgcctccg gtcggcggca acacgtcatt
     3901 ttgcagcatg tatgcgatct acgaggcgct gccgccggag atc
//
LOCUS       DATLJT010000821         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_739617,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000821 DATLJT010000000
VERSION     DATLJT010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739617"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1261)
                     /locus_tag="VM910_18590"
     CDS             complement(134..1261)
                     /locus_tag="VM910_18590"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HVI64567.1"
                     /translation="MVCPLCNYAAKFHSYQQRRVLTVHGAVQVQRAYYYCGRCKQSYM
                     PYDEALGLVDEISPGLMPLVCLAGTLLPFADAAEDVLKRFAGVRVSASTVLRCTEGEG
                     ERLRAQLKEGRMVEPTSREPKWTAPRKEGQPAAYVGLDAFSVPMQGAGASKAEHRMLY
                     TALLYTPDKEHTRYLVDFELDALAEQVRSQARAVGVAQVSDLIAVTDGGNGLEEALQR
                     HLAENLTTILDWYHAAEHVCDFAKVWYARDEQARTQWQQQAKEILYEQGSEALLAHLR
                     AIELPLHTSAEVQEELRKLIGYFEKNRHRTDYPTYRRKGWDIGSGPTEAGCKIIGERL
                     KGSGMRWVEDGAATVAALRALYISGGKLWDGFWSQPHRAAA"
     gene            complement(1270..1512)
                     /locus_tag="VM910_18595"
     CDS             complement(1270..1512)
                     /locus_tag="VM910_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64568.1"
                     /translation="MSAHPLSAVANETREQRIEAIMKELRPKIDEAVRKLVERAVDVP
                     EAEEFGTIDFEFRDAGQKLANDVRQASVASRKKRGT"
     gene            complement(1844..>2494)
                     /locus_tag="VM910_18600"
     CDS             complement(1844..>2494)
                     /locus_tag="VM910_18600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVI64569.1"
                     /translation="TLFLDEIGDLSANGQAKLLRVLEEKVVYRVGGTAPIPVDTRVLA
                     ATNRNLADIVRCGKFREDLFYRLTVVTLELPPLRERRDDVMVLAEHFMQQFCRDAGRK
                     PLKFSPEARKRLAQHEWPGNIRELRNLLERVAYLSQSERVEAGDLAIIQRAGKDQANP
                     YGELTLTEATDAFQRDHIQHAIERAKGNVSEAAKLLGLHRPNLYRKMKLLGMEAKI"
BASE COUNT          473 a          817 c          681 g          523 t
ORIGIN      
        1 gatgccgtcg gtaaacggaa ttgaggcaat gggtttttcg tccatgcaga cagattatcg
       61 agaacgcggt taagtggcga ccggccggcg atgcgacgtt ttcgcggggt gtgcgtcttt
      121 tacttgggtc acttcacgcg gcggcgcggt gaggttggga ccaaaatccg tcccagagtt
      181 tgccgccgct gatatagagg gcacgcagcg ctgccaccgt tgccgcgccg tcctcgaccc
      241 aacgcattcc cgaacctttg agtcgttcac cgatgatttt gcaccctgct tccgtagggc
      301 cgctgccgat gtcccatccc ttgcgccggt aggtcgggta gtccgtgcga tggcggtttt
      361 tctcgaagta gccgatcagc ttgcgtaact cctcctggac ttccgccgag gtgtgcagcg
      421 gcaactcaat cgcacgcagg tgggcaagca atgcttcgct cccttgctca tagagaatct
      481 ccttcgcctg ctgctgccac tgcgtccgcg cctgctcgtc gcgcgcgtac cacaccttcg
      541 cgaaatcaca gacgtgctcg gcggcgtgat accaatccag aatcgtcgtc aggttctcgg
      601 ccaagtgtcg ctgcaacgct tcctccaagc cgttgccgcc gtcggtcacg gcgatcaaat
      661 cgctcacctg cgcgacgccc accgcccgtg cctgggaacg tacctgctcc gccaaggcat
      721 cgagttcgaa atcgaccagg taacgtgtgt gttccttgtc cggagtgtac aagagcgccg
      781 tgtagagcat tcgatgttcg gctttgctcg ctcccgctcc ttgcatgggc acactgaacg
      841 catccagacc aacgtaggcc gcgggttggc cttcctttcg cggcgccgtc cacttcggct
      901 cgcgtgacgt cggttcgacc attcgccctt ctttcagttg cgcccgcaaa cgctcaccct
      961 cgccttccgt acaacgcagc acggtcgagg ccgacacgcg cactccggca aaacgcttca
     1021 gaacatcctc ggccgcgtcc gcaaacggca gcaaggttcc cgccaaacac accagcggca
     1081 tgagccccgg gctgatctcg tccacgagcc ccaaagcctc gtcataaggc atataactct
     1141 gcttgcaacg accgcaataa tagtacgccc gctggacctg caccgcgcca tgtaccgtca
     1201 aaacccggcg ttgttggtac gaatgaaact tggcggcata gttgcaaagc gggcacacca
     1261 cgctgcatcc tacgtacccc tttttttccg gctcgccacg ctagcttgcc ggacatcatt
     1321 ggccaacttc tgccccgcgt cgcggaattc gaagtcgatg gtcccaaact cctccgcctc
     1381 cggaacgtcc acggcccgct cgaccagctt gcgaacggct tcatcgatct taggccgaag
     1441 ctctttcatg atggcctcaa tgcgttgttc ccgggtctcg tttgctacag cactcagagg
     1501 atgcgcagac atggctcaac tcctgatggt gacgacaaag tgcagacttc cacctgtcct
     1561 atcagcctac cgcgcgacgc gacaaatacc cacccaagta acagctgcac acccgttcct
     1621 cggcccactg cgtcgattgc cgattaatgc caatcctctt ccgctggacg gatgcgaatt
     1681 ccaggctgcg cgaaattcgc aggtcgtcac aaaccagaga gttttccttg cccggcatag
     1741 catcgtgccg caggcattcg tttccgcagg aaaacaatgt ccataccacc atcataactt
     1801 atgtgtaaaa cacattcaag caacgctggg aaacttcgac cactcatatc ttcgcctcca
     1861 tgcccagcag cttcatcttg cgatagagat tcggccgatg caagccgagc agctttgcgg
     1921 cttcgctgac gttccccttg gcgcgctcga tggcgtgctg gatgtggtcg cgctgaaagg
     1981 catcggtcgc ctcggtcagc gtcaattcgc catacggatt tgcctggtcc ttgccggcgc
     2041 gttggatgat cgccaaatcg cctgcctcaa cgcgctcgct ctggctcaga taagcgactc
     2101 gttccaaaag attgcgtagc tcgcggatgt tgccgggcca ttcgtgctgt gccaggcgct
     2161 tgcgcgcctc ggggctgaat ttgagcggct tgcggccggc gtcgcggcag aactgctgca
     2221 tgaaatgctc cgccagcacc atgacgtcgt cgcgccgctc acgcaaaggc ggcagctcga
     2281 gcgtgacgac cgtcaggcgg tagaacaggt cctcgcggaa tttgccgcag cgcacgatgt
     2341 cggcgagatt gcgattggtg gctgcgagaa cgcgcgtatc gaccggaatc ggcgccgtac
     2401 cgccgacgcg atagacgacc ttttcctcca agacgcgcaa cagcttcgcc tgcccgttgg
     2461 cgctgagatc gccgatctcg tcgagaaaca gcgt
//
LOCUS       DATLJT010000822         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2120272,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000822 DATLJT010000000
VERSION     DATLJT010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120272"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VM910_18605"
     CDS             <1..513
                     /locus_tag="VM910_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVI64570.1"
                     /translation="PPNLEGTGEITMRDLLRNALRMRPDRIILGEIRGAEALDVLQAM
                     NTGHDGSMSTIHANSPREALTRLENMVGMTGINLPSRAVRTQIAAAVHLIAQVNRMRD
                     GIRRITHIMEIVGMEGETITTQELFGFQFQGETTEGMLRGVFKSNGIRPNFLPRAEYY
                     GLDRPLLEVI"
     gene            517..1512
                     /locus_tag="VM910_18610"
     CDS             517..1512
                     /locus_tag="VM910_18610"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVI64571.1"
                     /translation="MGLGLDPVTTIAVICAIGVALTFGLVAVALSESAASRRFDRRVN
                     TMRDRAQGVLASEAAATRSLSRQRRASAIDRLAGTWLPRRDLLAARLERTGRTISIGQ
                     YAMACAGLTVLAAIGLAVATRIGIVPSLVLGLLIGTAVPHLVIGRIGKRRLNAFINLF
                     PDAIDLIVRALRSGLPVTEAIVGAGHEIADPVGAELRLIENGMRMGRDLDSLLWDTAK
                     RIDAPEFRFFVIALSVQRETGGNLAETLANLSDVLRRRRQMQAKARAMASETRATTMI
                     LGGLPIAVIGVLSLTSPHYLVPLINDVRGIILDGVALTMLTTGVVIMNRMAKFEI"
     gene            1509..2501
                     /locus_tag="VM910_18615"
     CDS             1509..2501
                     /locus_tag="VM910_18615"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVI64572.1"
                     /translation="MRLQDFAITDWLPSGVSLDEAITVLAGLATLATVLAMWQVLRPN
                     SALERRLEEIGERKETLRQGALAAARRGRQRTTAVGVMRETVTRLNLLRSKHATEARN
                     MLAQAGIRSRDAMVGYLFARITLPFVLGIALLADNKVFHVLPIPPNFAFVLPFAGVLI
                     GFYAPIVYLRNAAAKRAKSIKLALPDGLDLMVICAEAGLSLDATLQRVSRELGHGNSE
                     LAEELGMTAAELTFLPERRLAFDNLNARTNSDGIRAVVNTLQQTAKFGTPLAQSLRVL
                     AAEMRQARMTRAEEKAARLPALLTVPMILFILPCLFIVLLGPAAIGLIDTFAHR"
     gene            2679..3101
                     /locus_tag="VM910_18620"
     CDS             2679..3101
                     /locus_tag="VM910_18620"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI64573.1"
                     /translation="MAEQRRIYRREGDGYPSDLKDAEWARLKPMIPSARPGGLPRKTD
                     MGAAMNAILYFLRTGCPWRDLPRDSFPPRSTVYDIFRKFQYDRVWETLWAELDMASRE
                     RMGREASPSAAVLDSQVIKSAEKGAVTTARWVTTPASR"
BASE COUNT          582 a         1026 c         1043 g          573 t
ORIGIN      
        1 ccacccaacc tggagggcac cggcgagatc acgatgcgtg acctgttgcg caatgctctg
       61 cgcatgcggc ccgaccgcat catcctcggc gagatccgtg gcgccgaggc gctcgatgtg
      121 ctgcaggcga tgaacaccgg ccacgacggc tcgatgtcga cgatccacgc caactcgccg
      181 cgcgaggccc tcacccgact ggagaacatg gtcggaatga ccgggatcaa cttaccctcg
      241 cgtgcggtgc ggacccaaat cgccgccgct gtccatctga tcgctcaggt caaccgcatg
      301 cgcgacggca tccgccgcat cacacatatc atggagatcg tcggaatgga gggtgaaacg
      361 attaccaccc aagagctgtt cggcttccag tttcaaggcg aaacgacgga aggcatgctg
      421 cgtggggttt tcaaatcgaa tggaattcgc ccgaattttt tgccgcgcgc cgagtattac
      481 ggcctcgatc ggccgcttct cgaggtcatc tgagacatgg gactggggct cgatccggta
      541 accacgatcg cggtcatttg cgcgatcggc gtcgcgttga cgtttggcct agtggcggtg
      601 gcgttgagcg aaagcgcggc aagccggcgc ttcgatcggc gtgtcaatac gatgcgagat
      661 cgggcgcagg gggtcctcgc ctcagaggcg gccgccactc gatcgctttc gcggcagcgg
      721 cgcgcgtcgg caatcgatcg cctggctggc acttggctgc cgcgccgcga cctcctcgca
      781 gcacgcctcg agcggaccgg gcggacgatc agcatcggcc agtacgcgat ggcgtgcgcc
      841 ggcctgaccg tactggcggc aatcggcctt gccgtcgcca cgcggatcgg catcgtgccg
      901 agcctggtgc tcggattgct gatcggaacg gccgttccgc acctggtcat tggacgcatc
      961 ggaaagcgcc gcctgaatgc cttcatcaat ctgttcccgg atgcgatcga tctgatcgtg
     1021 cgggcgctgc gttcgggcct gccggtcacc gaagcgatcg tcggagcagg ccatgagatc
     1081 gccgatccgg tcggcgccga gctacgcctc atcgaaaatg ggatgcggat ggggcgagac
     1141 ctcgacagcc tgctgtggga caccgccaag cgcatcgatg cgccggaatt ccgcttcttt
     1201 gtcattgctc tcagcgtgca gcgcgagacc ggcggcaacc ttgcagaaac tctcgccaat
     1261 ctctccgatg tcctgcgccg gcgccgacag atgcaggcca aggcgcgcgc gatggcaagt
     1321 gagacgcgcg caacgacaat gatcctcggc ggattgccga tagcggtcat cggagtgctg
     1381 tcgctgacct caccccatta tctggtgccg ctcatcaacg atgtccgggg aataatcctc
     1441 gacggcgtcg cgctgacgat gctgacgacc ggcgtcgtga tcatgaacag aatggcgaag
     1501 ttcgagatat gaggcttcaa gatttcgcga tcaccgactg gctgccgtcc ggggtcagcc
     1561 tcgatgaggc aatcacggtg cttgccgggc tcgccacgct ggcgacggtt ctggcgatgt
     1621 ggcaggtgct gcggccgaat agcgcgctcg agcggcgtct cgaagagatc ggggaacgca
     1681 aagaaaccct gcggcaaggc gctcttgcgg cggcacgccg aggccgacag cgcacgaccg
     1741 ccgtcggagt gatgcgcgag acggtgacgc gcctcaacct gctgcgttcg aaacacgcca
     1801 ctgaggcgcg caatatgctg gcccaggccg ggatccgatc gcgggacgca atggtcggct
     1861 acctctttgc cagaatcacc cttcccttcg tgttggggat tgcgctgctc gccgacaaca
     1921 aggtgtttca cgtgctcccg atcccgccca atttcgcgtt cgtgctgcca tttgcggggg
     1981 ttctgatcgg gttctacgct ccgatagtct atctgaggaa cgccgcggcc aagcgggcca
     2041 agagcatcaa gctcgccctg ccggacgggc tcgacctgat ggtcatctgc gccgaggccg
     2101 ggctcagcct cgatgcgact ctacagcgcg tctcccgcga gctcggccac ggcaattccg
     2161 agcttgccga agaactcggc atgactgcgg ccgaactgac ctttctaccg gagcgccgct
     2221 tggcgttcga caacctcaat gcacggacca actccgacgg cattcgcgcc gtcgtcaata
     2281 cgttgcagca gaccgccaaa ttcggcactc cgctggccca gtccctgcgt gtcctcgccg
     2341 ccgaaatgcg gcaggcgcgg atgacccggg cggaggagaa ggcagcacgg ctaccggcgc
     2401 tgctgaccgt gccgatgatc ctcttcatct tgccgtgcct gttcatcgtg ctgctcggcc
     2461 cggcggcgat cggcctcatc gacacattcg cgcaccgctg actgcgaccg ctagcctcca
     2521 tcccgttcga gagacggatc agccgcgtct attcgggcct cggctaaagc agatcccacc
     2581 gcttcacctg agagcatgac ccaaaggttt gcgacgtcgc gtcggatggc atgatacgcc
     2641 ggctacaggc ctcacggtga tggcgaacag cgatgtggat ggcggagcag cgccggattt
     2701 accggcggga aggcgatggc tatccgagcg acctgaagga tgcggagtgg gcgcggctga
     2761 agccaatgat cccatcggcc cggcctggcg ggctaccgcg caagaccgac atgggcgcgg
     2821 cgatgaacgc cattctctat tttctgcgca ccggctgccc ctggcgcgac ctgccgcgcg
     2881 acagctttcc gccacgctcg acggtctacg acatctttcg caagttccag tatgatcgcg
     2941 tgtgggagac gctctgggcc gagctggata tggcgtcgcg cgagcgaatg ggccgggagg
     3001 ccagtccctc agcggcagtt ctcgatagcc aggtgatcaa atcagcggaa aaaggggcgg
     3061 taacgacggc gaggtgggtt acgacgccgg caagcaggtg aagggccgca agatccacgc
     3121 cctggttgac agcgaggggc tgccgatgcg ggtcattgtc cactcggccg ccattcagga
     3181 tcgtgacggg gccggcttgg tgctcgacaa gatacgccag cgtt
//
LOCUS       DATLJT010000823         3560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1891224,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000823 DATLJT010000000
VERSION     DATLJT010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891224"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VM910_18625"
     CDS             complement(<1..1738)
                     /locus_tag="VM910_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64574.1"
                     /translation="MSIGFWDIESRSTVALDIAGAWRYAADPTTEILCVAYAIDDGDP
                     QIWVPGDPPPQELLAADEIVAHNFAFERAMATRILTTPHGWPTIPLAKQRCSMTLALA
                     CALPPALDKAAKALGLPYEKDAEGYRLMRRMSRPRRPHKNEDPSKTYWVDGPEQREQL
                     HRYCMRDVETERALYRRLPPLLPSEQELWQLDAVINQRGFFVDTALTIAARDIARAEQ
                     AALNVEVAQITDGEILSLNQVEKIKDFIRRHGHAIESLTRRSAAQVLRHPSDTVRQLL
                     ELRHAGARASTRKFDALLASVDTDSRLRGTLKFHASSTGRWAGNRFQPQNLKKPETKD
                     LDAAIDAILTGDMAKVRELGAPLTVAGDVSRAIISAAPGHALIGGDFSAIESRILAWL
                     AGEKWKLRNYEDYDRTGNAEFEPYCIMASRALKRQVTPEDEVGRNFGKTYDLAFGFGG
                     GLGAWRRFDDSDTYTDAEVEHFKQEFRRTHLETIRFWRRLDHAAHKAVITRQRVDFNR
                     FSFTMEDGTLLLTLPSGRRLHYPEVRLVPGKFEDTRQLRFKDNSRGAWSDVDAWYGVL
                     VENVVQATARDIL"
     gene            complement(1814..2587)
                     /locus_tag="VM910_18630"
     CDS             complement(1814..2587)
                     /locus_tag="VM910_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64575.1"
                     /translation="MSELAKKLKSETALVNLDGFDGYTDEYEGEEHDQDQFTSGRVIQ
                     GTRINFTNEATWVDISKQQLPADLQLIVAEVLRIVQKWGHDNMPAEPPIILSSNQKWP
                     NIRAMNEQCPKTEWRMHFGKLTGPYQAQKIVYLWDPVTMNKYSWPTSSNSGMACVSEL
                     VEKIAMMRQFKRQKAIPVVKLGSRLWSKRYNKQGPNLIVVQWIVKGEGGTLLPITEPA
                     AISGPGSTTTTQEVLDTFAGVKTVEPPTGKEATNDEIKF"
     gene            complement(2584..2985)
                     /locus_tag="VM910_18635"
     CDS             complement(2584..2985)
                     /locus_tag="VM910_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64576.1"
                     /translation="MKILGADERLAHAWRKWHAEQLKAALEGVHGAVLERLMAQLKDL
                     HSARELVDFIAAQDWSVVDTDTRFTALHEINIAITKLRERNGQEPISDPLPGAPENAF
                     RIIKNLFKSFPPRAGERAEAIGIGKNMEKSA"
     gene            complement(2989..3291)
                     /locus_tag="VM910_18640"
     CDS             complement(2989..3291)
                     /locus_tag="VM910_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64577.1"
                     /translation="MTRERLPNRRPATTFDFTCGNLHYACSYSRFASGGIAELFLSNH
                     KTNSHADTAARDAAITFSIAVQHGADPETIRSALCRDSHGRASGPLAAVLDLTMGE"
     gene            complement(3288..>3560)
                     /locus_tag="VM910_18645"
     CDS             complement(3288..>3560)
                     /locus_tag="VM910_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64578.1"
                     /translation="RRIALTRKQVKGLPSFPAADKRKDPRYKWFRSNYGDRCWELDAM
                     DPNDLRVCVERAIVALIEPVAWQRCEIINQAERESLQTILQKWGGS"
BASE COUNT          603 a         1045 c         1118 g          794 t
ORIGIN      
        1 ccaggatgtc gcgcgcggtc gcctgcacca cgttctcgac cagaaccccg taccaggcgt
       61 cgacgtcgct ccacgcgccg cgggagttgt ccttgaagcg gagttgtcgg gtatcttcga
      121 atttcccggg gacaagcctg acttccggat agtggagccg gcgtccgctc ggcagcgtca
      181 gcaacagggt cccgtcttcc atcgtgaacg agaaccggtt gaagtcgacg cgttggcgag
      241 tgatgacggc cttgtgggcg gcgtggtcga gccggcgcca gaaccggatc gtctccagat
      301 gggtgcggcg aaactcctgc ttgaagtgtt cgacctcggc gtcggtgtag gtgtcgctgt
      361 cgtcgaagcg gcgccaagcg ccgagcccac cgccgaagcc gaaggcgaga tcataggtct
      421 tgccgaagtt gcgtccgact tcgtcctcgg gggtgacttg tcgcttgagc gccctagaag
      481 ccatgatgca atagggttcg aactcggcgt tgccggtgcg gtcgtagtcc tcataattgc
      541 gcagcttcca cttttcgccg gccaaccacg ccaagatgcg gctttcgatc gcactgaaat
      601 cgccgccgat tagcgcgtgg ccgggtgctg cgctgatgat ggcgcgggag acgtcgccgg
      661 cgaccgtcag cggggcgccg agttcgcgca ccttggccat gtcgccggtg aggattgcgt
      721 cgattgcggc gtcgaggtcc ttggtctccg gctttttcag attctgcggc tgaaatcggt
      781 tgcccgccca ccggccggtc gagctggcgt gaaatttcaa ggtccctcgg agccgactat
      841 cggtgtcgac gctggcgagc agggcatcga atttgcgggt cgaggcgcgc gctccggcgt
      901 gtcgtagttc aagcagctgg cgaacggtgt cggagggatg ccgaagaact tgagctgccg
      961 aacggcgggt tagactttcg atcgcgtgtc cgtgtcggcg gatgaagtcc ttgatcttct
     1021 cgacttggtt gaggctgagg atctcgccat cggtgatctg ggcgacttcg acattgagag
     1081 cggcttgctc tgcgcgggcg atgtcgcgag cggcgatggt cagcgcggta tcgacgaaga
     1141 atccgcgttg gttaatgacg gcgtcgagtt gccacagttc ttgctcgctg ggcagcaatg
     1201 gcggcagacg ccgataaagc gcgcgctcgg tctcgacgtc gcgcatgcag tagcggtgca
     1261 attgttcgcg ctgttccgga ccgtcgaccc aataggtttt gctcgggtcc tcgttcttgt
     1321 gcgggcgccg cggtcgcgac atgcgccgca tcaggcgata gccctcggcg tccttttcgt
     1381 acggaagccc gagcgcctta gcggctttgt ccaacgccgg gggcaaggcg caggccagcg
     1441 ccagtgtcat gctgcagcgc tgcttcgcga gcgggatcgt cggccagccg tggggcgtcg
     1501 tcagaatgcg tgtcgccatc gcgcgctcga atgcgaagtt gtgggcgacg atctcgtcgg
     1561 cggctaatag ctcctgcggg ggaggatcgc cggggaccca gatttgcgga tcgccatcgt
     1621 cgatggcgta ggcgacgcac aaaatttctg tggttgggtc cgcggcgtag cgccacgcgc
     1681 ccgcaatgtc gagcgcgacg gtcgagcgag actcgatgtc ccagaagccg atcgacacct
     1741 ggaaaatctc cgcgaagtcg cattccgatg ccgtcccgcc ttccaaaggg tggcatcaag
     1801 ctgcggctcc gcgtcaaaat ttgatctcgt cgttagtcgc ctctttgccg gtcggcggtt
     1861 cgacggtctt cacacccgcg aacgtgtcca acacctcttg ggtggtggtg gtggaccctg
     1921 ggccagaaat cgcggccggt tccgtgattg gcagcagggt cccaccctca cccttcacga
     1981 tccactgtac gacgataagg ttcggacctt gcttgttgta tcgcttggac cagagccgag
     2041 agcccaactt gaccacaggg atcgctttct gtcttttgaa ctgtcgcatc attgcgattt
     2101 tctcaacgag ctccgagaca caggccattc cagaattgga gctggtcggc cacgaatact
     2161 tgttcatggt gaccgggtcc cacaaataga cgattttctg tgcctgatac ggccccgtga
     2221 gcttgccaaa gtgcatgcgc cactcggtct ttgggcactg ctcgttcatg gctcggatgt
     2281 ttggccactt ttgattagag ctgagaatga tcggcggctc cgccggcatg ttgtcgtggc
     2341 cccacttctg gacgatacgc agcacttcgg cgacgatcag ctgcagatcg gccggcagtt
     2401 gttgtttgga gatgtcgacc caggtcgctt cgtttgtgaa gttgatcctc gtaccctgaa
     2461 tcactcttcc gctggtgaat tgatcctggt cgtgctcttc accttcatat tcgtcggtgt
     2521 atccgtcgaa accgtccaag ttgacgagtg cagtttccga tttcagtttt ttcgctaact
     2581 cactcatgct gacttctcca tgtttttacc tatgccgata gcctcggctc gctcccccgc
     2641 acgcggcggg aaactcttga acaggttctt gatgatccgg aacgcgttct ccggggcacc
     2701 aggcagcgga tcggagatcg gctcctgccc gttgcgctca cgcagcttag tgatcgcgat
     2761 gttgatctcg tggagcgccg tgaaccgcgt gtcggtgtcg accacgctcc aatcctgcgc
     2821 agcgatgaag tcgaccaact cgcgcgctga atgcaggtct ttgagctgcg ccatcagccg
     2881 ctcgagtacg gcgccgtgta caccttcgag cgcggccttt aattgttcgg cgtgccactt
     2941 gcgccaggcg tgtgcgaggc gctcgtccgc tccgagaatt ttcattgctc attcccccat
     3001 ggtgagatcg agcaccgcag ccagcggccc gctggcgcga ccgtggctgt cgcgacagag
     3061 cgcactgcga atggtttcgg gatcagcgcc gtgctgaacc gcgatcgaga acgtgatggc
     3121 ggcatcgcgc gcggcagtgt cggcgtgtga attggtcttg tgatttgaga gaaatagctc
     3181 ggcgattcca ccgctggcaa agcgactata cgagcatgca tagtgaagat ttccgcacgt
     3241 gaaatcgaac gtcgtggcag gcctccggtt cggaagacgc tcgcgagtca tgacccaccc
     3301 cacttttgga ggatcgtctg cagcgactcc cgctctgctt ggttgataat ctcgcagcgt
     3361 tgccacgcta cgggctcgat caacgccacg attgcccttt caacgcagac gcgaagatcg
     3421 tttggatcca tcgcatcaag ttcccagcaa cggtctccgt aattggagcg gaaccatttg
     3481 tagcgcgggt ctttgcgctt atccgctgct gggaacgacg gtagtccttt gacctgtttt
     3541 cgcgtcagcg caattcgcct
//
LOCUS       DATLJT010000824         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_512831,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000824 DATLJT010000000
VERSION     DATLJT010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512831"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /gene="cpaB"
                     /locus_tag="VM910_18650"
     CDS             complement(<1..460)
                     /gene="cpaB"
                     /locus_tag="VM910_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVI64579.1"
                     /translation="MVSGSDNKPAPAEPVAQLPTAEVLVAKSDIALGQTVRPEDLQWQ
                     TWPAATASSSFIRRSDRPNAVTQVAGAIARAPFITGEPIREQKLVKADGSGFMAAILP
                     TGMRAVSTEIAPETGAGGFILPNDRVDVILTRHVKNPERPSLPDVVVSEIV"
     gene            complement(769..1710)
                     /gene="gshB"
                     /locus_tag="VM910_18655"
     CDS             complement(769..1710)
                     /gene="gshB"
                     /locus_tag="VM910_18655"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HVI64580.1"
                     /translation="MKLNVAVQMDPIARINIGGDSTFALLLEAQKRGHGLSYYTPDKL
                     SLVGDELFAPVQLLTVRDEPGNHFTLGEPKREPISSFDVVLLRQDPPFDLAYITSTHF
                     LERIQPKTLVVNDPAAVRNAPEKLFVMNFPQLMPPTLISRDLDEINAFRDTHGAVVMK
                     PLHGHGGAAVFRVMPQDMNFGSLFDMFSVAFKEPWVTQRFLPEVKHGDKRIILVDGEF
                     AGAVNRVPAADDLRSNMVRGGAARATDLTPREREICTTIGPTLRELGLLFVGIDVIDG
                     NLTEVNVTSPTGIRAIARLGGPDIAAKIWDVIEKKRG"
     gene            complement(1739..2140)
                     /locus_tag="VM910_18660"
     CDS             complement(1739..2140)
                     /locus_tag="VM910_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HVI64581.1"
                     /translation="MFGSERVAPPRDAAPERVAAFRTGLSAESRAAAYLMAKGYRILA
                     KRFRTPHGEIDIVAKRRNLLAFVEVKARATLDDAAFAVTPRQQARIIDAAQAWLMAHP
                     EHAEFELRFDAILIAPRHLPRHLLAAFDASS"
     gene            complement(2127..3080)
                     /gene="rsmI"
                     /locus_tag="VM910_18665"
     CDS             complement(2127..3080)
                     /gene="rsmI"
                     /locus_tag="VM910_18665"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVI64582.1"
                     /translation="MRTKPAPINTESAREPDGRSFSVAGELLTAPRAEAGLHLVATPI
                     GNLGDITLRALQTLAAVDIIACEDTRITRRLMERYGIAAELMLYHEHNAAAVRPKILE
                     HLAQGGSVALVSDAGTPLISDPGFKLVREAAAAGYKVTALPGPSSVLTALSVSALPTD
                     RFFFEGFLPAKRAARRSRLSGLARIDATLVLFESGNRVQETLADLAEILGAREAAICR
                     ELTKLYEEVVRAPLAELAARAGGIETRGEFVLVIGPPQTDAQMTAAALDDLLRDELER
                     HSVKDAVAHAVELSGRPRREIYARALELVRETTARGDHVRE"
     gene            3320..>3611
                     /locus_tag="VM910_18670"
     CDS             3320..>3611
                     /locus_tag="VM910_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HVI64583.1"
                     /translation="MVGPFNPRSGAAGRDVPGPRTLGATRRTAIGLMLGAPLLSACSG
                     IQQTFSQFTSSETPGPAGPAQQPSVAGTGQVKVGLILPMSASGNAGVAAQSMK"
BASE COUNT          661 a         1104 c         1201 g          645 t
ORIGIN      
        1 ggacgatctc ggacacgacg acgtccggca gcgaggggcg ttccggattc ttgacatgcc
       61 gggtcaggat cacgtcgacc cggtcgttcg gcaggatgaa accgcctgcg cccgtctcag
      121 gcgcgatttc ggtcgagacc gcacgcatgc cggtcggcag gattgctgcc atgaagccgc
      181 ttccgtcggc tttgaccagt ttctgttcgc ggatcggctc gcccgtaata aagggcgcgc
      241 gcgcaatcgc gcccgccacc tgggtgacgg cgttgggacg atcgctgcgg cggatgaagc
      301 ttgagctcgc ggttgccgca ggccaggtct gccattgcag gtcctcgggc ctgacggtct
      361 ggccgagggc aatgtcggat tttgcgacga gaacctcggc cgtcggcaat tgcgcaacgg
      421 gctcggcggg tgcgggtttg ttgtcggagc cgctcaccag gtaggcggca acgccgccgg
      481 caccaagggc gatgatcagg acgacaattc gggccgtata cgcttactat ctgctcttac
      541 tcccgtaccc atgagcagga ctaagcagca aaggtataag ccggcttgaa gcggggaaca
      601 gattgcgccc gcagatcagg gattcgtccg catggtgaac gagacgttat cgttcgacgg
      661 acaaagctgc gtatatttcc cgatcgacgc gggcggacat tgcggcgtca gcgccgcgtt
      721 cagagattgg caaccacaac atatcgaagg caggaggcgg gctgctccct agcctcgctt
      781 cttctcgatg acgtcccaga ttttggcggc gatatcggga ccgccgagcc gcgcgatggc
      841 gcggattccg gttggcgagg tgacgttgac ctcggtcaga ttgccgtcga tcacatcgat
      901 gccgacgaac aacaggccga gctcgcgcaa ggtcggtccg atggttgtgc agatttcgcg
      961 ctcgcgcggc gtcaaatcgg tggcccgcgc cgcaccgcca cgcaccatat tggagcgcag
     1021 atcatcggcc gccggcacgc gattgaccgc cccggcaaac tcgccgtcga ccaggatgat
     1081 gcgtttgtcg ccgtgcttga cctcgggcag gaagcgctgg gtgacccacg gctccttgaa
     1141 agccaccgag aacatgtcga acagagagcc gaagttcatg tcctgcggca tgacacgaaa
     1201 cacggccgcg ccgccatggc catgcagggg cttcatcacg acggcgccgt gggtatcgcg
     1261 gaaggcgttg atctcgtcga gatcgcgcga aatcagcgtc ggcggcatga gctggggaaa
     1321 attcatcaca aacagttttt ccggcgcgtt gcggacggcc gccggatcat tgaccaccag
     1381 cgtcttcggc tgaatgcgct cgagaaaatg ggtcgaagtg atgtaggcaa ggtcgaacgg
     1441 cgggtcttgc cgcagcagca ccacatcgaa ggacgaaatc ggctcgcgtt tgggttcacc
     1501 gagggtgaaa tggttgcccg gctcgtcgcg cacggtcagg agctgaaccg gcgcgaaaag
     1561 ctcgtcaccg acgagcgaaa gcttgtccgg cgtgtaatag gacagcccat gaccgcgctt
     1621 ttgcgcttcg agcagcagcg cgaaggttga atcgccgccg atgttgatgc gcgcgatagg
     1681 atccatctgg acggcgacgt tcaatttcat caggtgagcc cggggtgtga ggacatcttc
     1741 aggagctggc atcgaatgcc gctaacagat ggcgcggcaa atggcgcggt gcaatcagga
     1801 tcgcgtcaaa acgcaattcg aattcggcat gctcgggatg cgccataagc caggcctgcg
     1861 cggcatcgat gatgcgcgcc tgctggcgtg gcgtgaccgc gaaggcggcg tcatccagcg
     1921 tggcgcgggc cttgacctcg acaaacgcca acaggttgcg gcgtttggcg acgatatcga
     1981 tctcgccatg cggcgtgcga aagcgctttg cgagaatccg gtagcccttg gccatgagat
     2041 aggccgcagc gcggctttcc gccgacagac cggtgcggaa cgccgccaca cgttcgggtg
     2101 cggcatccct cggtggagca acacgttcac tcccgaacat ggtcgcccct tgcagtggtt
     2161 tcccggacga gctccagcgc gcgggcatag atttcgcggc gcggccggcc ggagagttcg
     2221 acggcgtgcg caaccgcgtc cttgacgctg tgccgctcga gctcgtccct caacagatcg
     2281 tcaagcgcag cggccgtcat ctgcgcatcc gtctgcggtg ggccgatgac cagcacgaac
     2341 tcgccgcgcg tctcaatccc gcctgcgcgg gccgcgagtt cggcaagcgg ggcgcgcaca
     2401 acttcttcgt atagtttggt cagctcgcgg caaattgcag cctcgcgcgc gcccaaaatt
     2461 tcggcgagat cggcaagcgt ctcctgcacg cgattgccgg attcgaacag caccagcgtg
     2521 gcgtcgatcc gcgcgagtcc ggacagccgg ctgcggcgcg cggcccgctt tgccggcaga
     2581 aaaccctcga agaaaaaacg gtcggtcgga agggccgaga ccgacagcgc cgtcagtacc
     2641 gacgagggcc ccggcaatgc cgtcaccttg tagcctgcgg ctgcagcctc acgcaccagc
     2701 ttgaagccgg ggtccgagat cagaggcgtg ccggcgtccg acaccagcgc gaccgagccg
     2761 ccctgcgcaa ggtgttccag gatcttcggc cgcactgctg cggcattatg ctcatgataa
     2821 agcatgagct ccgcggcgat gccgtatcgc tccatcagtc gtcgggtgat gcgggtatcc
     2881 tcgcaggcga ttatgtcgac ggcggcgagc gtctgtagcg cccgcaacgt gatatcgccg
     2941 aggttgccga tcggcgttgc caccagatgg aggccggctt cagcccttgg cgcggtgaga
     3001 agctcgcccg cgaccgaaaa gctgcggcca tcgggttccc tggcgctttc ggtatttatc
     3061 ggggcaggtt ttgtgcgcat aatgctacct taagggggag aaagcctcgg cgccaccttg
     3121 caagcctgag ctgtgcggcc gtttttgcgg ccgacaacgg cgaagcgacg aaagcggtgc
     3181 gaccaggggc cgttcggcgg cggccacaat ccttttgttt tggttaacta ttcgccgaga
     3241 atatgcctga atccgcgctc ccgaccgtat ttaagggggc agtcctgacc ggtcgaggtc
     3301 gatcaagcga agagatgtga tggtgggccc gttcaatccc aggtccggcg ctgcgggtcg
     3361 tgacgtcccg ggtccgcgca cgcttggtgc gacccggcgg acggcgatcg ggttgatgct
     3421 cggcgcgccg ttgctttcgg cttgttccgg catccagcag acctttagcc agttcacgag
     3481 ctcggagacg ccggggccgg ctggaccggc ccagcagccg agcgtcgccg gcacgggcca
     3541 ggtcaaggtc ggattgatcc tgccgatgtc cgcctcgggc aatgccggcg tcgccgcaca
     3601 atccatgaag a
//
LOCUS       DATLJT010000825         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_53306,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000825 DATLJT010000000
VERSION     DATLJT010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53306"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM910_18675"
     CDS             complement(<1..471)
                     /locus_tag="VM910_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI64584.1"
                     /translation="MSEQIAAGKGASSGQLRMSQRVVNLAYMLTQNARRHGDRTGFVW
                     GENAWTWREIDAEVSALAAALAARGIQKGDRILVHSKNGEEMFWSMFAAFRLGAVWVP
                     TNFRLMPDEVAYLASASGAKAFLCHGDFPDHAKAVSGPAFTWRIGTDSTFGERSV"
     gene            complement(468..668)
                     /locus_tag="VM910_18680"
     CDS             complement(468..668)
                     /locus_tag="VM910_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64585.1"
                     /translation="MKTVLTVLGVVLLVIAATYFLVPADQLPGFFPGHEAGVTRVHMK
                     HGVVSGVAGFILLGAGVWMGRR"
     gene            852..1133
                     /locus_tag="VM910_18685"
     CDS             852..1133
                     /locus_tag="VM910_18685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64586.1"
                     /translation="MSDIIDNRAHHRFELDVDGHVAMSFYKLADGVITFVHTEVPPEL
                     GGKGIGSRLIQGALDQVRAEGLKVVAQCPFVKAWIGKHPEYQGLLKADS"
     gene            1228..1500
                     /locus_tag="VM910_18690"
     CDS             1228..1500
                     /locus_tag="VM910_18690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64587.1"
                     /translation="MASVRDNKPKNRFELDVEGQTAFANYRLTPRAVIITHTETPRNL
                     RGRGIASELVKGALDMIRADGQKVIAGCGFVVDYLRKHPEYADLVG"
     gene            complement(1513..2301)
                     /locus_tag="VM910_18695"
     CDS             complement(1513..2301)
                     /locus_tag="VM910_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI64588.1"
                     /translation="MKSIDRRKVLAASGALALTGAATAPAAAQASAKPIFPVPITTIP
                     IVGETDVFQVRRIYCIGRNYAAHAIERGSDPNREPPFFFQKPTDAIQDVAIGQVADHP
                     YPSLTKNYHHEVELVAALKSGGTNIPVHLALEHVYGYALGLDMTRRDLQNGMAAEKKP
                     WEIGKSFDHAAVLGPIHPISKVGHLTKGAISLAVNGAVRQNSDLDKMIWSVAEQIEKL
                     SEAFELKAGDIIYSGTPENVGPVVKGDVLLCKLDGLADMSIRIV"
     gene            complement(2383..>2469)
                     /locus_tag="VM910_18700"
     CDS             complement(2383..>2469)
                     /locus_tag="VM910_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 family protein"
                     /protein_id="HVI64589.1"
                     /translation="ASMNVWLHKQVMAKLAANGGKLPPEIKH"
BASE COUNT          483 a          785 c          750 g          451 t
ORIGIN      
        1 caccgagcgc tcgccgaaag tactgtcggt tccgatccgc caggtgaaag cgggccccga
       61 cacggccttg gcatgatcgg gaaagtcgcc atggcacaaa aaggcttttg caccggaagc
      121 gctggcgaga taggcgactt catccggcat cagacgaaaa ttggtgggca cccacaccgc
      181 tccgagccgg aacgccgcaa acatcgacca gaacatctcc tcgccattct tggagtggac
      241 gaggatccgg tcaccttttt gaatgccgcg cgcggcgagc gccgccgcga gcgccgaaac
      301 ttctgcgtcg atctcgcgcc aggtccaggc gttctcaccc cagacgaagc ctgtgcggtc
      361 gccatgacgg cgcgcgttct gggtcagcat ataggcgagg ttcacaaccc gctgcgacat
      421 gcgaagctgc ccgctggaag cgcccttccc cgctgcgatt tgctcgctca tctgcgcccc
      481 atccagacgc cggccccaag caggataaag ccggccacgc cggacacgac cccgtgcttc
      541 atatgcacgc gcgtcacgcc ggcctcatgt cccggaaaga agccaggcaa ctggtccgcc
      601 ggaaccagga aataggtggc ggcaatgacg agcaggacga cgccgagcac ggtcagcacg
      661 gtcttcatgg ggtttcctcg atccaattgg gacgcgcggt acgcgacccc cttgcaattc
      721 tgtttatcgc cttcccgcga tgccccgcaa gggcggccgg gaccggcccg cgcgcctgct
      781 cgccgttatc agcggatgta tggtaccaat ggcgggagca cgtggcatcg ccaaacctca
      841 cggcaacaga catgagcgac atcatcgaca acagggcaca tcaccgcttc gaactcgacg
      901 tcgatggcca tgtcgccatg agcttctaca aactcgccga tggcgtgatc actttcgtcc
      961 ataccgaggt gccgccggaa ctcggcggca aggggatcgg ctcgcgactg atccagggtg
     1021 cgctcgacca ggtgcgtgcc gaaggattga aggtcgtcgc gcaatgcccg ttcgtcaaag
     1081 cgtggatcgg caagcatccg gagtatcagg gcttgctgaa ggctgacagc taaagcgcgg
     1141 caggatcgat ccgcagacct cgcgcctttg catcggcggg ttcgcgcgcc tacatgacgc
     1201 aacagatcct ctggggaaat ccagccaatg gccagcgtcc gcgacaacaa gccgaaaaac
     1261 cgttttgagc tcgatgtgga gggccagacc gccttcgcca attaccgcct gacgccgcgc
     1321 gcggtgatca tcacccatac cgagacgccg cggaatttgc gcggccgcgg catcgcctcc
     1381 gagctggtga agggcgcact cgacatgatc cgtgccgacg gacagaaggt gatcgccggc
     1441 tgcggttttg tggtggacta cctgcgcaag caccccgaat atgctgacct cgtcggctag
     1501 cgaaccggcc gatcacacaa tgcggatcga catatcggca agcccgtcga gcttgcacag
     1561 aagcacgtcg cccttcacga ccgggccgac attttccggc gtgccggagt agatgatgtc
     1621 gccggctttc aactcgaagg cctcggagag tttttcgatc tgttcggcaa cgctccagat
     1681 catcttgtcg agatccgagt tctgccgaac cgctccgttc acagccagcg agatcgcgcc
     1741 cttggtgaga tgaccgactt tggaaatggg atggatcgga ccgagcacgg cggcatggtc
     1801 aaagctcttg ccgatttccc acggcttctt ctccgccgcc attccgttct gcagatcgcg
     1861 ccgcgtcatg tcgaggccaa gcgcgtagcc atagacatgc tcgagcgcga ggtggaccgg
     1921 aatattggtg ccgccggatt tcagcgcggc gaccagctcg acttcgtggt ggtaattctt
     1981 cgtgagcgag ggatagggat gatccgcaac ctgcccgatc gcaacatcct ggatcgcgtc
     2041 ggtcggcttc tggaaaaaga acggcggttc gcggttcgga tcggagccgc gctcgatcgc
     2101 gtgcgccgcg tagttgcgtc cgatgcagta gatgcggcgc acctggaata cgtcggtctc
     2161 gccgacgatc gggattgtcg tgattggcac cggaaatatc ggttttgcag aggcctgcgc
     2221 cgcggccgga gccgttgcgg ctcccgtcag cgccagcgct cccgaggcgg ccagcacttt
     2281 ccgacgatca atggatttca taggcgttct cccgtcagtg gtcctcgcaa aaaaaggccc
     2341 gcgttgccac gggcctttga cctgtctgct gcgaaaaatc aactaatgct tgatttccgg
     2401 aggcagcttc ccgccgttgg cggcgagctt cgccatcacc tgcttgtgca gccagacgtt
     2461 catgctggc
//
LOCUS       DATLJT010000826         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_512867,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000826 DATLJT010000000
VERSION     DATLJT010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512867"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VM910_18705"
     CDS             complement(<1..1218)
                     /locus_tag="VM910_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI64590.1"
                     /translation="MHEIAGWLKKLGLEQYAQLFAENGIDFSVLPDLTEQDLEKLGVL
                     LGHRRKMLRAIAELEARTSFDKAALESARADDIERRHLTVMFCDLVGSTALSARLDPE
                     DLRTVVKAYHRCCAELIVKSGGFVAKYMGDGVLAYYGYPEAHEEDAERAVRAGLAVIE
                     AVAKLDTDAKSALRVRVGIATGLVVVGERLGEGVSQEQAVVGETPNLAARLQALAQPD
                     TVMIDGTTHRLLGDLFEYRALGNEIVKGFDHPVPVWHVIGVSGLDSRFEALRTTTTAL
                     VGREEEIELLMRRWQQVKGGDGCVVLISGEPGVGKSRVCQAIFERLSSDPHTRLRYFC
                     SPHHQNSAFYPIITQLELAAGIRREDLVEQRLDKLEAVLAQAIREPRDAAPLLAALLS
                     IPTRERYPSLNLSP"
     gene            complement(1554..2459)
                     /locus_tag="VM910_18710"
     CDS             complement(1554..2459)
                     /locus_tag="VM910_18710"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64591.1"
                     /translation="MRRIGLLLTATADGPESLARVGAFLQGLQELGWTDGRNVRLEYR
                     WGSADAERIRKSAAELVALAPDVILVSGNSGMAPLHRATRTVPIVFVNTSDPVGGGFA
                     MSLARPGGNATGFSSIEYGMSGKWLELLKQISPSVTRAAVLRDPSIPSGSGQLGAIQA
                     VGPFLGVEISSIDARDAGEIERGVTDLARSANGGLIVPASTPTIFHRKLIIALAAQHR
                     LPAIYPYRFFVADGGLVSYGADSVDPFRRAAGYVDRILKGEKPGDLPIQAPTRFELVI
                     NLKTAKALGLEVPPTLLARADEVIE"
     gene            complement(2583..>2928)
                     /locus_tag="VM910_18715"
     CDS             complement(2583..>2928)
                     /locus_tag="VM910_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HVI64592.1"
                     /translation="LGGYSAIAAVANDLLPRLRADPQLGRFWAHRGEDGIMREKQLLI
                     DFLCASAGGPMYYRGRDMTLTHRGMRISDSDWNVFLGHAAATLAKFQVPEAEQREVVA
                     FVQSLKKECVER"
BASE COUNT          575 a          943 c          862 g          548 t
ORIGIN      
        1 tgggctcaga ttgagcgacg gataacgttc gcgggtcgga atcgagagca gtgctgcgag
       61 cagcggcgcg gcatcacgcg gctctctgat tgcctgggct aggaccgctt ccagcttgtc
      121 cagccgttgc tcgaccaagt cctcgcgccg aattccggcc gccagctcga gctgggtgat
      181 gatggggtag aaggcgctgt tctggtggtg cggtgagcaa aagtaacgca atcgcgtgtg
      241 cggatcactg ctcaatcgtt caaatatagc ctgacatacg cgcgacttgc cgacgcctgg
      301 ctcgcccgat attagcacaa cgcagccgtc gccgccctta acctgctgcc accgccgcat
      361 tagcaattcg atctcctcct cccggccaac cagtgcagta gttgtcgtgc gcaaggcttc
      421 gaagcgacta tcgaggccgc tcacgcctat aacgtgccaa accggcaccg gatggtcgaa
      481 accttttacg atctcgttgc cgagagcgcg gtattcgaat agatcgccga ggaggcggtg
      541 agtggtaccg tcgatcatca ccgtgtccgg ctgagccaac gcctgaagcc gagccgcaag
      601 attgggagtc tcaccgacca ctgcctgctc ctgcgagacg ccctcgccga ggcgctcgcc
      661 gacgactacc agaccagtgg caattccaac tcgtactcgc aaagctgatt tcgcatcggt
      721 gtccagctta gctacggcct caatcactgc taatcccgcg cgtaccgctc gctcggcatc
      781 ttcctcatgt gcttccggat agccgtagta cgcgagtacg ccatcaccca tgtatttcgc
      841 gacaaatcct ccagatttga cgatcagctc tgcgcaacat cgatgatagg ctttgacaac
      901 cgtgcgtaga tcctcggggt cgaggcgcgc agagagcgcg gttgacccga cgaggtcgca
      961 aaacatcacc gtgagatgac ggcgttcgat gtcatccgct cgcgcagatt cgagcgcagc
     1021 tttatcgaag gaagttctgg cctccagctc ggcaatcgca cgcaacatct ttcggcgatg
     1081 cccaagcaaa acgcccaact tctctaaatc ctgttctgtc aaatcaggaa gtacgctgaa
     1141 atcgatcccg ttctccgcaa agagctgggc gtattgctcc aagccgagct tcttgagcca
     1201 gcccgcaatc tcgtgcatct agcgagcgag tccctgaaaa accctacgct acctgcgttc
     1261 caccttgggc ggaagctctt ggggttcgcc ggtcagctcc aaagcctgct cggcgagctc
     1321 ttggtaggtg ctgtgcaaaa cagcatagcg cgccaaagga aagtggtggg cggtgcggca
     1381 atgtgcgccg cacaaatccc gagcggccaa tgtctcagtt gggtcaaacc cggtcctctg
     1441 ccgatgtccg ccgcatgacg gcttgaccct cgtaagcgga cctagccgga tcacgtcgtg
     1501 atgtctcaca ggtcccatta gctgccgtga gcagatgcag caaatagcac cgcctattcg
     1561 atcacctcgt cggcgcgggc gagcagcgta ggcggaactt cgaggccgag cgccttggcc
     1621 gtcttgaggt tgataacaag ctcgaacctg gtgggcgcct ggatcggaag gtcgcccggc
     1681 ttctcgccct tgaggatgcg gtcgacgtag ccggccgcac gccggaatgg gtcgaccgag
     1741 tcagccccgt aggagaccag gcccccgtcg gcgacgaaga agcggtaggg atagatcgcc
     1801 ggcagccggt gctgcgccgc cagcgcgatg atcagcttgc gatgaaatat ggtcggcgtg
     1861 ctcgccggca cgatcaggcc gccgttcgcc gagcgcgcca aatcagtgac gccgcgctcg
     1921 atctcgccgg cgtcgcgcgc gtcgatcgaa ctgatctcga cgccgaggaa cggccccacg
     1981 gcctggatcg cgcccaactg cccgctcccg gacgggatgc tcggatcgcg gaggaccgcc
     2041 gcgcgggtga cgctgggcga aatctgcttg agcaactcga gccattttcc gctcatgccg
     2101 tattcgatgc tggaaaaccc cgtcgcattg ccgccaggac gcgctaggct catggcgaaa
     2161 ccaccgccga ccgggtcgct ggtattcaca aacacgatcg gcacagtacg agttgcccgg
     2221 tgcagcggcg ccatgcctga gttgccactg accaagatga cgtccggcgc gagcgcgacc
     2281 aattccgccg cagatttgcg aatgcgttca gcatcagcgg agccccagcg atactccagc
     2341 cgcacattgc ggccatcggt ccaacccagt tcctgcaagc cctgcaggaa tgctccgacg
     2401 cgggcaagcg attccggacc gtccgctgtc gccgtcaaga gcagcccgat gcggcgcatc
     2461 cgttcaggct gctgcgcgcg cgccgcgagc ggccaccggc ggttccgccg agcatcgtga
     2521 tgaactcgcg ccgtttcact cgatcacctc gtcggcaagc gcgagaagtt gcaaagcgct
     2581 cttcaacgtt cgacgcattc ttttttgagg ctctgaacaa acgcgaccac ctcacgctgt
     2641 tcggcctcgg gcacctggaa cttcgccaag gtcgcggcgg catgaccaag gaacacgttc
     2701 cagtcgctat cgctaatccg catgcctcgg tgcgtgaggg tcatgtccct gccgcgatag
     2761 tacattggtc cacccgcgct cgcgcaaaga aaatcgatga gaagctgctt ttcgcgcatg
     2821 atgccgtctt ctccgcggtg cgcccagaac cgcccgagtt gcggatcggc tcggagcctg
     2881 ggcagaagat cattcgccac tgctgcgatt gcgctatatc caccaagg
//
LOCUS       DATLJT010000827         7890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_739904,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000827 DATLJT010000000
VERSION     DATLJT010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739904"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VM910_18720"
     CDS             complement(<1..323)
                     /locus_tag="VM910_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64593.1"
                     /translation="MKTIALAVTTMLLLAAITMTAMPQSQTGNTAYEKKSYNYAEWAK
                     GRFSEVVTVRNAGKIIYLGGVGAEDENSAQGGVIRHLGDFGAQCRYAWDKIKRLLEKH
                     GATVD"
     gene            569..1447
                     /locus_tag="VM910_18725"
     CDS             569..1447
                     /locus_tag="VM910_18725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64594.1"
                     /translation="MTIVVPFGAGGNTDMMARLAAQHLSAKFGQSFVVENRPGAGGAL
                     GTGQVAAAAPDGYLLLFAASSMIVLTPQLQKLNFDPMTQLVPITNVGIGTQMIAIKRA
                     LPAETLPQFIAYAKANPGKLNFTVAGTQNISHLAPVLLFARAGIELVMVPAKSEPQAV
                     SDLISGQVDLYFGNSSALLPHVNNDKIRLIAVGTAQRIAAAPDIPSVAETLPGFEFSS
                     WNGFLAPAGTPEPIITAIRNEVTALAKSPEISDRLSKLGIVPGGLPKEATEAVFKKDF
                     ESFAAAIKAAGIEPAK"
     gene            1466..2335
                     /locus_tag="VM910_18730"
     CDS             1466..2335
                     /locus_tag="VM910_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HVI64595.1"
                     /translation="MPTPAQRLRELLSGPNVEIMPGCYDALSAKLVADAGYKITFMSG
                     FAVSAARLGMPDTGLVSFAEMLDSLHNCCAAASPVPLIGDGDTGYGNALNVQRTVIEY
                     ARAGAAGVMIEDQVSPKKCGHTRGKQVIPRAEARMKIRAAVDARADADILIMARTDAR
                     AVCGFEEALARCKDFEAEGADIIFLEAPETEDEMRRFCGAMTKPCMANMVAGGKTPVL
                     PLQKLQEVGYKLALYPVMLLSSAIAAMQATLAALCPECNLPQPRAISFAELQSVVGFP
                     TYWERETKYQLKD"
     gene            complement(2296..2988)
                     /locus_tag="VM910_18735"
     CDS             complement(2296..2988)
                     /locus_tag="VM910_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVI64596.1"
                     /translation="MSRNDLEIYRSQGFGGTVGIGRKPALCIVDFVNGFVDPAIFGGG
                     NIAESIARTIPLLAAARLRGLPVAFSRVVYREGGRDDCAFVRKVPALAMLTETAPMGQ
                     IVPQLAPLPDETILRKTQPSAFFGTGYADLLRRQGVDTVIVAGCTTSGCVRATVVDAM
                     SHNFRTIVARDGVGDRSLAAHEANLFDMEQKYADLMDAAALVAWVNRAAAARSVAPMD
                     QSLSWYFVSRSQ"
     gene            complement(3009..4208)
                     /locus_tag="VM910_18740"
     CDS             complement(3009..4208)
                     /locus_tag="VM910_18740"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI64597.1"
                     /translation="MSSRNERIIVVGAGPVGLTAALALGRRGIPTVLLAAEPELVTEL
                     RGSTFHPPTLDLLDELEIVPRMIEVGLKAPTWQFRDRETGPVATFDLSLLAGDTNHPY
                     RVQCEQWKLMCFLEAELRRLPGADIRFSHEVTSVRQDGDAVAVTAETPSGPVSIGGRY
                     VIAADGARSAVRRSLGVEFEGFTYPELFLIASTDFKFEKTLTDIAYVNYIADPLEWLV
                     LLRVPELWRVLVPAPENSDREKLLSDESLQGVLHRVVPQPEPYKIAHRSIYQVHQRVA
                     KSFRHGRVVLAGDAAHINNPLGGMGMNGGIQDAFNLADKFKDIWAGADDRLLDRYDRQ
                     RRTVAVEAVQEHTHRNQQIISERDPKTRRKSLDAMRRIAADKAQARDYMLKSSMIGSM
                     RRAAEIQ"
     gene            4283..5035
                     /locus_tag="VM910_18745"
     CDS             4283..5035
                     /locus_tag="VM910_18745"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HVI64598.1"
                     /translation="MRDVLGWRCKFGVLGPSTNTIVQPDFEMMRPAGVTNHYSRIFTP
                     NAQAVSNDTFMAGTQVIGQNTLDAVRSVMTCSPDHLVMGMSAVTFYGGAKGADEFQGK
                     VEKESGVGVSIGSHSCTAAMKTYGGVKRIAVLSPYYPIANEQVARYFTDSGFEVVRDI
                     CLQCKSWTAIAEVPPELLRGKLIELNGNGRDVDAIVQVGTNLSMVRLAAAAEMWLGKP
                     VIAINTATYWHALRRNKIMDKIPGLGRLLEEF"
     gene            5127..6032
                     /locus_tag="VM910_18750"
     CDS             5127..6032
                     /locus_tag="VM910_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64599.1"
                     /translation="MKYKFVPISQRKPLRWPNGKRLAVMITTNLEYWDVTKDTPKPFY
                     PGGPGIVGGDLPGNVYDNPNWTWREYGQRVGVWRMFDIFDEFGVPSTCTMNAKIGLER
                     RAVIDAALKRGWEIIAHNYVQTELLADYSFDENKEREVIRETLRVYKEVCGKPAKGWL
                     SSSLRCTLNTIDLIAEEGLIFTTDLLNDDQPYLVETRSGRTIVSIPYTSEVNDFTVFM
                     RQGQDVNGAFAVFKEGFDWLYRESAVSGRFMNIGLHPHVVGQPFRIRALYDFIAYAKS
                     FDDVWFATREEIAEWYLKNHHSHIA"
     gene            5996..7225
                     /locus_tag="VM910_18755"
     CDS             5996..7225
                     /locus_tag="VM910_18755"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI64600.1"
                     /translation="MVSEKSPLPHRVMNIDAPTAKGQATDPVRRARQLRPLIEAEAPA
                     VERGCALTPKLLAALHDARLFKMLLPHSVGGDEIDPLTFITVIEEIARADGSTAWCIA
                     QASGCSMAAAYVEPAVAKEIFGGKDAVLAWGPVGPTVKATAVEGGYSATGTWSYASGS
                     RHAQWLGGHSPLVDAEGKPCLGPDGKPTERTMLFPKSQATIRDVWQVMGLKGTGSDSY
                     TVADLFVPARYTFTREAAADRRERGPLYRFTTYQIYGSSFASVALGIARATLDAFMQI
                     ASTKVPMLGSKPLRDNPVVQSKVAVAEAQWQSSRAFLMQALGDMWDTACRRQGFTLRQ
                     RAALRLAAVHAGHQAKEVVETAYHFAGGTAIFENQPFERRLRDVHAATQQVQAQFVNF
                     EVAGQVLLGLPVTSKLI"
     gene            7376..>7890
                     /locus_tag="VM910_18760"
     CDS             7376..>7890
                     /locus_tag="VM910_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI64601.1"
                     /translation="MRFVVGLCALLLAVVIGHAAVASPEGKRVMLLGTANTNPYIGAW
                     TSTFIKFATQASMKVTNLSSNYDAAVQSQQVDDAIAQKFDLIAVVYVNDQAIIPALTR
                     AKAAGIPVMLIVTPLKKEYEELFTSYIGTDHAELGRLAAEGMIKGLQAEGKTKAQVVA
                     VTGAAQQLNVQT"
BASE COUNT         1508 a         2411 c         2470 g         1501 t
ORIGIN      
        1 ttgtccacgg tcgctccatg cttttccaat agccgcttga ttttgtccca ggcatagcgg
       61 cactgcgcgc cgaaatcgcc gaggtgccgt ataacgccgc cctgagcgct gttttcatct
      121 tccgctccga ccccgcctag gtaaatgatc tttcccgcgt ttctcacggt cacgacctcg
      181 gagaagcgcc ccttcgccca ttcggcatag ttgtagctct tcttctcgta ggccgtgttg
      241 cccgtttggg attggggcat cgctgtcatg gtgatggcag cgagcaagag catcgtcgtc
      301 acggcgaggg cgatcgtctt catgatattc ctcccatgtg tacgcgaagg aacggcacga
      361 accggacgat gatatcacca cagttttgtt tgtgtacggg cccggatttg ccgaccttgc
      421 caaccgatcc gaagtgcgcg aggcttctcg gagttcaacg ggaaatgata tgcatcgcgc
      481 gctgagactg ctcgtcgtcc tctcgggtct agcggcgatt gccctgttat ccgggtcgat
      541 tgccgccgca gattggcctt cgagaccggt gaccattgtc gtgccgttcg gtgcgggcgg
      601 caacaccgac atgatggccc ggctcgcggc gcagcatttg tctgccaaat tcggccagag
      661 cttcgtcgtc gaaaatcgtc cgggcgcagg cggcgcgctc ggtaccgggc aggtcgcggc
      721 ggcggcaccc gatggatact tgctgctgtt cgctgcctct tcgatgattg tgctgacgcc
      781 gcagttgcag aagctcaact tcgatccgat gacgcagctc gtgccgatca ctaacgtggg
      841 catcgggacg cagatgatag ctatcaagcg ggcactgccg gcggaaacac tcccgcaatt
      901 cattgcctat gcaaaggcca atcccggtaa gctcaatttc accgtggccg gcacgcagaa
      961 catcagtcat cttgcacctg tcctgctttt cgcgcgtgcc ggcatcgaac tggtcatggt
     1021 tccggccaaa agcgagccgc aggcggtctc tgatctcatt tccggtcaag tcgatcttta
     1081 tttcggcaac tcctccgcgc tgttgccgca tgtcaacaat gacaagatcc ggctgatcgc
     1141 ggtcggaacc gcgcaacgga tcgcggcggc cccggacatt ccttcggtcg ccgagacact
     1201 gccgggattc gagttctctt cctggaacgg gttccttgca ccggccggga ccccggagcc
     1261 gatcattaca gcgattcgga acgaggtgac cgccttggcg aagtcgccag agatttccga
     1321 ccggctgagc aaacttggga tcgtgccagg cgggctgccg aaggaagcga ctgaggcggt
     1381 ctttaagaag gacttcgagt cgtttgcagc ggcgatcaag gctgcgggaa ttgagcctgc
     1441 aaaatagcac aaaaagaggt gcaccttgcc gacaccagcc cagcgactgc gcgaactgct
     1501 gtccggaccg aacgtggaga tcatgcccgg ctgctacgat gcgctctcag cgaagctcgt
     1561 cgcagatgcc ggctacaaga tcacattcat gagcggcttt gcggtatcgg ccgcgcggct
     1621 cgggatgccc gacaccggcc tagtttcgtt tgccgaaatg ctcgacagcc tgcacaattg
     1681 ctgcgctgcc gcaagcccgg tgccgttgat cggcgatggc gataccggct acggcaacgc
     1741 ccttaacgtc cagcgcacag tgatcgaata cgcacgtgct ggagccgccg gcgtgatgat
     1801 cgaggaccaa gtcagcccga agaaatgcgg ccacacccgg ggcaaacagg tgatcccgcg
     1861 tgcagaggcg cggatgaaga ttcgcgccgc agtggacgcg cgtgccgatg ccgacatctt
     1921 aatcatggcg cgcactgacg cgcgtgccgt gtgtggcttc gaggaagcgc ttgcacgctg
     1981 caaggacttc gaagccgaag gtgcggatat catcttcctc gaagcgccag agacagagga
     2041 tgagatgcgg cgtttctgcg gggcgatgac gaaaccgtgc atggccaata tggtggcggg
     2101 cggcaagacg ccggtcctgc cgctgcaaaa attgcaggag gtcggctaca agcttgcgct
     2161 gtaccccgtg atgctgctgt cttcagcgat tgccgcgatg caggcaacac ttgcggcgct
     2221 gtgcccggag tgcaaccttc cgcagccgcg cgcaatttca ttcgcggaac tgcaaagcgt
     2281 cgtcggcttt cccacttact gggaacgcga gacgaagtac caactcaagg attgatccat
     2341 tggcgccacc gatcgggccg cggctgcgcg attaacccac gcgacgagcg ccgcggcgtc
     2401 catgaggtcc gcgtatttct gctccatgtc gaacagatta gcctcgtgcg cggcgagcga
     2461 ccgatcgccg acgccgtcgc gcgccacgat ggttcggaaa ttgtggctca tggcgtcgac
     2521 caccgtcgcg cgcacgcagc cggaggtcgt gcagcccgcg acgattaccg tgtccacgcc
     2581 ctgccgccgc agcaggtcgg catagccggt gccgaaaaac gccgatggct gggtcttgcg
     2641 caggatcgtc tcgtccggca acggggccag ctgcggcacg atctggccca tcggcgccgt
     2701 ctcggtcagc atcgccagcg ccggaacctt acgcacgaag gcgcagtcgt cccggccccc
     2761 ctccctgtag accacgcgcg agaacgcgac cggcaagcca cgcaggcgcg ccgcggccag
     2821 caatggaatt gtcctggcta tcgactcggc gatgttgccg ccgccgaaga tcgcgggatc
     2881 tacgaagccg ttgacgaaat cgacgataca caacgcgggc ttccggccga tcccgaccgt
     2941 tccgccgaat ccctgggagc gatagatttc gagatcgttc ctgctcatcc catgcccctt
     3001 gaagcgttct actgaatttc cgcggcgcgg cgcatgctgc cgatcatcga ggactttagc
     3061 atatagtcgc gcgcctgcgc cttatcggcg gcaatccggc gcatcgcgtc gagcgacttg
     3121 cggcgggttt tgggatcgcg ctcgctgatg atctgctgat tgcggtgtgt gtgctcctgc
     3181 accgcctcaa ccgctacggt gcggcgttgg cggtcgtagc ggtcgagcag ccggtcatcg
     3241 gcgcctgccc agatatcctt gaatttatcc gcgaggttga aagcatcctg gatgcccccg
     3301 ttcatgccca tgccacccag cggattgttg atgtgcgcgg catcgccagc aagcaccacg
     3361 cggccgtgcc ggaagctttt tgcaacgcgc tgatgcacct gatagatcga gcgatgggcg
     3421 atcttgtaag gctcgggttg cggcaccacg cggtgcagca cgccctggag cgattcgtcg
     3481 gagagcagtt tttcgcggtc ggaattttcc ggcgcgggca ccagcacgcg ccagagctcc
     3541 ggcacccgca gcagcaccag ccattccagc gggtcagcga tatagttcac ataagcgata
     3601 tcggtcagtg tcttctcgaa cttaaaatcg gtcgatgcga tcaggaacaa ctcgggatag
     3661 gtgaagccct cgaactcgac gccaagcgag cgccgcactg cgctgcgcgc gccgtcggcg
     3721 gcgatcacgt agcggccacc gatcgacaca ggacccgatg gcgtctcagc cgtcacagcc
     3781 acggcgtcgc cgtcttgccg caccgacgtg acctcgtggc tgaagcggat gtcagcgccg
     3841 ggaagcctgc gcagctcagc ctcgaggaag cacatgagct tccattgttc acattgcacg
     3901 cgatagggat ggttggtgtc gcccgcgagc aaggacaggt caaaggttgc gaccggccca
     3961 gtctcgcggt cgcggaactg ccaagtcggc gctttcagcc cgacctcgat catgcgcggc
     4021 acgatctcaa gttcgtccaa aaggtcgagc gtcggcggat ggaacgtcga gccacggagc
     4081 tccgtgacaa gctcgggctc ggccgcgagc agcaccgtcg gaataccgcg gcggccgagc
     4141 gcaagcgcag ccgtcaggcc gacggggccg gcgccgacaa caatgatgcg ttcgttccga
     4201 cttgacatcg atgacgtact tccgattgac agccagatat agcaaaatgg gccgcgtaaa
     4261 tctcgaaggg ggcactccgg cgatgagaga tgttctcggt tggcggtgca agttcggcgt
     4321 gctcggacct tcgaccaata ccattgttca gcctgatttc gagatgatgc ggccggccgg
     4381 cgtcaccaac cattacagcc ggatcttcac acccaacgcc caggcggtca gtaacgacac
     4441 gttcatggcc ggcacccagg tgatcggcca gaacacactc gacgccgttc gcagcgtgat
     4501 gacctgctcg ccggaccatc tggtcatggg catgtcagcg gtgacgtttt atggcggcgc
     4561 caagggggcc gatgagttcc agggcaaggt cgagaaggag tccggggtag gcgtcagcat
     4621 cggttcgcat tcctgcaccg cggcgatgaa gacctacggc ggcgtcaagc ggatcgcggt
     4681 tctatcgccc tactatccga tcgccaacga gcaggtcgcg cgatacttca ccgattcggg
     4741 cttcgaggtc gtccgcgata tctgtcttca atgcaagagc tggacggcga tcgccgaagt
     4801 cccgccagag ctgctccgcg ggaagctgat cgagctcaac ggcaacggcc gcgatgtgga
     4861 tgcgatcgtt caggtcggca ccaatctcag catggtgcgc ctcgccgcgg cggcggagat
     4921 gtggcttggc aagccggtca tcgccatcaa tacggcgact tattggcacg cgcttcgccg
     4981 gaataagatc atggacaaaa ttccagggct tgggcgcctg cttgaggagt tttgaagctt
     5041 gtccgcaaat tcgggtcgat gtttgataag agaccacgaa aacgcgttgt ttcgtcgggg
     5101 tgctttacag ggcgggaatg tgatccatga aatacaaatt tgtgccgatt tcccagcgga
     5161 agccgcttcg gtggccgaac gggaagcgac tcgctgtcat gatcacgacc aatctcgaat
     5221 actgggacgt gactaaggat acgccgaagc cgttctatcc cgggggtccc ggtatcgtcg
     5281 gcggcgatct gccgggaaat gtctatgaca acccgaactg gacgtggcgc gaatacggcc
     5341 agcgggtcgg cgtctggcgc atgttcgaca tcttcgacga gttcggcgtg ccatcgacct
     5401 gcacgatgaa cgccaagatc ggcttggagc gacgggcggt gattgacgca gcgctcaagc
     5461 gcggctggga gatcatcgct cataactatg tgcagaccga gctgctcgcc gattattcgt
     5521 tcgatgagaa caaggaacgc gaggtgatcc gcgagaccct gcgggtgtac aaagaggtgt
     5581 gcggcaagcc ggcgaaaggc tggctgtcca gctcgctgcg ctgcaccctc aacaccatcg
     5641 acctaatcgc cgaggaaggc ctgattttca ccaccgatct gctcaacgac gatcagcctt
     5701 atctggtcga aacgcgctcg gggcgaacga tcgtttcgat accctacaca tcggaagtca
     5761 acgacttcac tgtgttcatg cgccagggcc aggacgtgaa cggcgctttc gctgtcttca
     5821 aggaaggctt cgattggcta taccgcgaaa gtgcggtcag cggccggttc atgaacatcg
     5881 gcctgcaccc gcacgtggtc ggtcagccgt tccgcatccg cgcgctgtac gatttcattg
     5941 cctatgcgaa gtcattcgac gatgtttggt tcgccacccg cgaggagatc gcggagtggt
     6001 atctgaaaaa tcaccactcc cacatcgcgt gatgaacatc gacgcgccga ccgccaaggg
     6061 ccaagccacc gatccggtcc ggcgggcgcg acagttgcgt ccgctgatcg aggcggaggc
     6121 gccggcggtc gagcgcggat gcgcgctgac gccgaagctt ctggccgcgt tgcatgacgc
     6181 gcgcctgttc aagatgctgc tgccgcacag cgtcggcggt gacgaaatcg atccactgac
     6241 gttcatcact gtgatcgagg aaatcgccag agccgacggc agcaccgcct ggtgcattgc
     6301 gcaggcgtcg ggctgctcga tggccgccgc ctatgtcgaa cctgccgtgg caaaggagat
     6361 cttcggcggc aaggatgccg ttctcgcctg gggcccggtc ggaccaaccg tcaaagcgac
     6421 ggcggtcgag ggcggctata gcgcgacggg gacctggtcc tacgcgagcg gcagtcgtca
     6481 tgcgcagtgg ctcggcggac attcgccgct ggtcgatgcc gaaggaaagc cgtgccttgg
     6541 tcccgatggc aagccgactg agcgcaccat gctgtttccg aaaagccagg ctaccattcg
     6601 cgacgtctgg caggtcatgg gcctcaaagg caccggaagc gacagctaca cggtggcgga
     6661 tctgttcgtg ccggcgcgct acaccttcac ccgggaagcg gcggccgatc gccgcgagag
     6721 aggcccgctt tatcggttca ccacctatca gatctacggc tcgtcgttcg ccagcgtggc
     6781 gctcgggatc gcgcgcgcga cgctcgatgc ttttatgcag attgccagca ccaaggtgcc
     6841 gatgctcggc tcgaagccgt tgcgcgacaa tccggtggtt caatccaagg tcgcggtcgc
     6901 cgaagcgcaa tggcagtcgt cgcgcgcctt cctgatgcag gcgctgggcg acatgtggga
     6961 tacggcctgc cggcggcagg gtttcacctt gcggcagcgt gccgcgctgc ggcttgctgc
     7021 cgtgcatgcc ggccatcagg cgaaagaggt ggtcgagacc gcctatcact tcgccggagg
     7081 cacggcaatt tttgaaaacc agccgtttga acggcggctg cgcgatgtgc acgcggcgac
     7141 ccagcaggta caggcccaat tcgtcaactt cgaagtggcc ggtcaggtgt tgctcggact
     7201 tccggttacg tcgaaattga tctgatgttg caatacgagg tcgagcggct ggccgcctgt
     7261 gcgcagcggc ttgcagaagc accatccggc aatcctgcgt cgcatcgcct cctggtgcac
     7321 ttgcctgact gacgctaaca ttttgcggtt cttggccttg gaatgggaga tatccatgcg
     7381 cttcgtggtg ggtctgtgtg cgcttctgct cgcagttgta atcgggcacg ccgccgttgc
     7441 gtcgcccgag ggcaagcgtg tgatgctgct cggcaccgcc aataccaatc cttatatcgg
     7501 cgcctggacc tcgaccttca tcaagttcgc gacgcaggcc agcatgaagg tgacgaatct
     7561 cagctccaac tacgacgccg ccgtgcagtc gcagcaagtc gacgatgcga tcgcgcagaa
     7621 gttcgacctc atcgccgtgg tgtacgtcaa cgaccaggcc attatcccgg cgctgacgcg
     7681 ggccaaggcc gcgggaattc cggtgatgct gatcgtcact ccgctgaaga aggagtacga
     7741 ggaactcttc acctcctata tcgggaccga tcacgctgag ctcggacgtc tcgctgcgga
     7801 gggcatgatc aaaggactgc aggcagaggg taagaccaag gctcaggtgg tagcggtcac
     7861 cggcgccgcg cagcagctca acgtccagac
//
LOCUS       DATLJT010000828         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3039905,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000828 DATLJT010000000
VERSION     DATLJT010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039905"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VM910_18765"
     CDS             <1..277
                     /locus_tag="VM910_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI64602.1"
                     /translation="DNAAVANLIGYSYRKLGDYKLSQVWYERALKADPNHVKTWQYYG
                     LWQVEQGNRDQAQYHLNKIAQLAGTDSEEYRSLAAALEKTPGTGLVY"
     gene            305..856
                     /locus_tag="VM910_18770"
     CDS             305..856
                     /locus_tag="VM910_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI64603.1"
                     /translation="MRKSAIKLIALATFSLALVSAPVITTVYAAPDNDAPAPSPTKGK
                     GKKQKGSSLAPSQEYAKFANGYRAAYATIYDRHDYATAIDQLKALDHDDTAAVANLIG
                     YSYRKLGDYKLAQVWYERALKADPNHVKTWQYYGLWQVEQGNRDQAQYHLSKIAQLVG
                     TDSEEYRSLAAALANPPGTGLVY"
     gene            complement(909..2450)
                     /locus_tag="VM910_18775"
     CDS             complement(909..2450)
                     /locus_tag="VM910_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HVI64604.1"
                     /translation="MVLPALRYLAVVLALIAACPVVAVAQDAAPSDAGTPPTKGGARS
                     GGSPLPAAAELHKLPPDSTTKQVLDLPGRTLAFTATAGSIRLFDDKGEPQADIAYTAY
                     QLDGADRATRPVTFLFNGGPGAASAWLQLGNNGPWRLDINADRVTPSAAPDLKPNAET
                     WLDFTDLVFIDPVGTGFSRFVATGDDVRKRFFSVDGDVAANAVTIRRWLEKHGRLLSP
                     KYVAAESYGGIRGPKLVRHLQTREGIGVKGLILISPVLDFREYSGSSILQYVSSLPTF
                     AAVARAQKRDVTRADMDDVERYARGDFLLDLMKGEADTEATNRLADKVASLTGIDQTT
                     SRRLGGRFDIAEFRREFDRRDGKVTGRYDASVTGYDPFPDSSFHHFGDPSGDPLVAPL
                     TSAAIDLVSRKLNWQPDGSYELLNGGVERAWDFGRGLSPLESITELRQILALDTKLRV
                     LVAHGLFDLAAPYFGSKILLDQLPQTFASPERLKFVVYPGGHMFYSRDAARKAFRAEV
                     EALMK"
BASE COUNT          665 a          996 c          983 g          588 t
ORIGIN      
        1 cgacaatgca gcggtcgcca atctgatcgg ctattcctat cgcaaactcg gcgactacaa
       61 gctgtcgcaa gtctggtacg aacgcgcgct gaaggccgac ccgaaccacg tcaagacctg
      121 gcagtattac ggcctctggc aagtcgaaca gggtaatcgc gatcaggcac aatatcacct
      181 gaacaagatt gcccaactcg ccggcaccga cagcgaggag tatcgttcgc tcgccgccgc
      241 gctggaaaag acgccgggca ccggccttgt ttattgagat cgacaattcc gacaggagac
      301 acgcatgcgt aagtccgcga tcaaactaat tgcgctggcg actttttcgc tggcgctggt
      361 gagcgcaccc gtcatcacga cggtttatgc cgcgccagac aatgacgctc ctgcgccctc
      421 ccccaccaaa ggcaagggca agaagcagaa gggttcctcg ctcgctccga gccaggagta
      481 tgcaaagttc gccaacggct atcgcgcggc ctatgccacg atctacgacc ggcacgacta
      541 cgccacggcg atcgatcagc tcaaggctct cgatcacgac gacaccgccg ccgtggcaaa
      601 tctgatcggc tattcctatc gcaagctcgg cgactacaag ctcgcgcagg tctggtacga
      661 gcgcgcgctg aaggccgatc ccaatcacgt caagacgtgg cagtattacg gtctctggca
      721 ggtcgaacag ggcaaccgcg accaggcgca atatcacctg agcaagatcg cgcaactcgt
      781 cggcaccgac agcgaggaat accgctcgct cgccgcggcg cttgcgaatc cgccgggtac
      841 cggtctcgtc tactgattgt cgatccagtt cgggaaagag gcgcaaggct atcgaccttg
      901 cgcctttttt acttcatcag cgcttcgacc tcggcgcgaa aggccttgcg ggcggcgtcg
      961 cgcgaataaa acatgtggcc gccgggatag accacgaact tgaggcgttc gggtgaagca
     1021 aaggtctgcg gcaactggtc gagcaggatc ttcgagccga aatacggcgc ggccagatcg
     1081 aacaatccat gggccaccag caccctcagc ttggtgtcga gcgcgaggat ctgccgtaac
     1141 tcggtgatcg attccagcgg actcaagccg cgaccgaaat cccaggcccg ctccaccccg
     1201 ccgttgagaa gctcgtagga tccgtcgggc tgccaattca gcttgcgcga gacgagatcg
     1261 atcgcggcgc tggtcagtgg cgccaccagc gggtcgcctg acggatcgcc gaaatgatgg
     1321 aagctcgaat ccgggaaggg atcgtaaccc gtcacggacg cgtcgtagcg tccggtcacc
     1381 ttgccgtcgc ggcgatcgaa ttcgcgacga aattccgcga tgtcgaagcg cccgccgagc
     1441 ctgcggcttg tggtctgatc gatccccgtc agcgaggcga ccttgtccgc cagacggttg
     1501 gtcgcctcgg tgtcggcttc gcccttcatc agatcgagca ggaaatctcc gcgcgcatag
     1561 cgctcgacgt cgtccatgtc tgcgcgcgtg acgtctctct tttgcgcgcg cgccaccgcc
     1621 gcgaatgtcg gaaggctcga cacatattgc agaatgctgg agcctgaata ttcgcgaaaa
     1681 tcgagcacag gcgagatcag gatcaatccc ttgaccccaa taccctcccg cgtctgcaag
     1741 tgacgcacga gcttgggtcc gcgaatgccg ccataactct cggcggcgac atatttgggt
     1801 gacagcaggc ggccatgctt ctccaaccag cgacggatcg tcactgcgtt cgcggcgacg
     1861 tcgccatcga cggagaagaa ccgcttgcgg acatcatcgc cggtcgcaac aaagcggctg
     1921 aagccggtcc cgacgggatc gatgaagacg aggtcggtga agtcgagcca ggtttcggcg
     1981 ttgggtttca gatcgggcgc ggccgaaggc gtgacccgat ctgcgttgat atcaagccgc
     2041 caggggccgt tgttgccgag ttgcagccag gccgaggcgg cgccgggccc gccattgaac
     2101 agaaatgtga cggggcgcgt ggcgcggtcg gcaccatcca gttgataggc ggtgtaggcg
     2161 atgtcggcct gcggttcgcc cttgtcgtcg aacaggcgga tcgagccggc cgtggctgtg
     2221 aaggcaagtg ttcgtcccgg aagatcgagc acctgcttag tcgtcgaatc cggaggcagc
     2281 ttgtggagtt cggcggccgc cggcaatgga gagcctcccg atcgcgcgcc gcccttcgtc
     2341 ggcggcgtgc ctgcatcgga gggcgcggcg tcttgcgcga ctgccaccac agggcaagcc
     2401 gcgatcagcg cgagcacaac ggcaagatag cgcaatgccg gcaaaaccat gcgtcgtcac
     2461 ctcccggatg tgccccgtac gaccgcatgc gcctgcgttt gcgcggccac agcggtttta
     2521 ctgtgaaaat tgcagcgcgc ttgcagcgtt ggcaaggaca gcaggtcaac ccgcacgcca
     2581 tctgtcgatc aaaagaaatc attttcgcgg gagcgcgcgc caacccccgg cgggttccga
     2641 tcaaatgcta aaagccgatg ccgacaatca gcacgaattg ccgtccgcac caggggccgg
     2701 tgcaatcaag attgctctgc agcggacgga tgtggcggac gtgtcgagat gccgcaacgt
     2761 gcgtcgccgc gctttaaggt tgcgctagga cgttcgtcga aagcgcggaa gaatccgacg
     2821 ggctcaaatt ggttccttct gttaaacaaa cacaagaaaa actgaagcat cgttgcaatc
     2881 tttgatcgct taatctccta aatccatctg aaagttgcag cggtctcagc gaagtgcttt
     2941 tcgaaggcgc acgcttggga tgtataatgc ggatctgccg ccggactttc gggtggccgt
     3001 gccgcacgag caccttacga tcttttttga accccgtccc gcgcgggtta cgaaatctca
     3061 cgctctcccc cgttctcgcc gatgaactcg agccaacggc cttgaaggtg ctgtgggtga
     3121 cctgcgtcgc taggtgtgcc tgcctgccgc gaccgggggg caacatcacg atcagtgcct
     3181 tcggcatgcg cgatcgggac gaacagcggt tttgattgcc caggacctca at
//
LOCUS       DATLJT010000829         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1202235,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000829 DATLJT010000000
VERSION     DATLJT010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202235"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_18780"
     CDS             complement(<1..230)
                     /locus_tag="VM910_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64605.1"
                     /translation="MEVRRNGRMVGEDRRWGVDQIASGVPDMPPFQKGVATMITKECF
                     GCEKVTSHEHLHDCAPGIPETHIVGSERFVCV"
     gene            complement(221..1867)
                     /locus_tag="VM910_18785"
     CDS             complement(221..1867)
                     /locus_tag="VM910_18785"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI64606.1"
                     /translation="MPMRRLSARGPRIDVFGRILALAMARHRDGRLAEAEQMYRQALR
                     WQPDQPDAYHLLGLLAAQLNRPDEALPLFERAIALNPAVPEYHANYGNALTIAARIDD
                     AEAAFAKAISLRPDFPEALMNLATLRRNRGDLDEAERLLHRSLALRPDWPDAQLNLAN
                     VALARQCFEEATTLFARALRAAPTYVHAYEAFARAAARAGRLQEAAAAFRRLLAFDPQ
                     NAVARHLLAACTCDPHYEKATEAYIRRLFDHYAPHFDSSLTQLQYQAPALIAQRLAQE
                     VAPDGSLTILDAGCGTGLCGALLRPFAARLVGVDLSAGMLKEAAKRELYDELIEAELG
                     AYMAAHPFTFDVIVACDTLVYHGRLDDAVAAAAKALKPRGRLLFTLEQLSEEGENPYR
                     LAPTGRFCHRVFYVQAMLAAAGLVDAKRRRYGRGHWGVQARDRLGDDKYYHADRNWFL
                     FYYLLASRDAYKQPSYTILGNHDWRFNPYPPLIKGALPTPDTLINNYEQFTQEEQRDI
                     LRKAHGPGYDRQFSYDLKARFLQKVESKDGLGCTTIREWR"
     gene            complement(2510..2734)
                     /locus_tag="VM910_18790"
     CDS             complement(2510..2734)
                     /locus_tag="VM910_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64607.1"
                     /translation="MKRREFITLLGGAGAWPLTARAQQPAMPMIGFLQSGSPGGTAHM
                     VAAFHSGLREVGLSFVLGAQRKPRACRGLR"
     gene            complement(2916..>3237)
                     /locus_tag="VM910_18795"
     CDS             complement(2916..>3237)
                     /locus_tag="VM910_18795"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVI64608.1"
                     /translation="IRTLDEAGLAGVRFVKADVEGGEREVLDGARATIARDRPIIMLE
                     LLSGTHENPAADVAAICESFGYEAFIVQRGEKIAALPAISVLVKNTSWGSEIESRNVL
                     FFPQ"
BASE COUNT          573 a         1017 c         1017 g          630 t
ORIGIN      
        1 aacacacaga cgaatcgttc cgagccgacg atgtgagttt ccggaatgcc gggggcgcag
       61 tcgtgcaagt gctcatgcga agtgactttt tcgcaaccga agcattcttt tgtgatcatg
      121 gtcgccaccc ctttctggaa cggcggcatg tccggtacgc cggaggcgat ctggtctact
      181 ccccaacgtc gatcttcccc gaccatccgt ccgttgcgcc tcacctccat tctctgatcg
      241 tcgtgcaccc gagtccgtcc tttgactcca ccttctggag gaagcgggcc ttcagatcgt
      301 aggagaattg gcgatcatag cccgggccgt gcgccttgcg taagatgtcc cgttgctctt
      361 cctgcgtgaa ctgttcgtag ttgttgatca gcgtgtccgg cgtcgggagg gcgcccttga
      421 tcaacggcgg gtaggggtta aaccgccagt catgattgcc gaggatcgta tagctgggct
      481 gcttatacgc gtcgcgcgag gcgagcagat agtagaagag gaaccaattt cggtccgcgt
      541 gatagtactt gtcgtcgccg aggcgatccc tcgcttgtac cccccaatga ccgcgaccat
      601 agcgtcggcg cttcgcatca acgagcccgg ctgctgccag cattgcctgc acatagaaca
      661 cacggtgaca aaagcgcccg gtcggtgcga gccggtaggg attctcaccc tcttccgaca
      721 gctgctccaa ggtaaacagc aatcggccac ggggcttgag cgccttggct gccgccgcga
      781 cggcgtcgtc gagccggccg tggtagacca gtgtgtcgca ggccacgatg acgtcgaacg
      841 taaatgggtg cgcggccatg taggcgccga gctcggcttc gataagctcg tcgtagagct
      901 cccgtttggc ggcttccttc agcatcccag cagagagatc cacgcccacg aggcgcgcgg
      961 cgaacggcct tagcagcgcg ccgcacaatc cggtcccgca gcctgcatcg aggatagtga
     1021 ggctgccgtc cggtgcgacc tcttgagcca ggcgctgtgc gatcagcgcg ggtgcctggt
     1081 attgcagctg ggtaagtgag ctgtcgaaat gcggcgcgta gtgatcgaac aaccggcgaa
     1141 tgtaggcttc agttgccttc tcgtagtggg gatcgcaggt gcacgctgcg agcaggtgac
     1201 gcgcgacggc gttttgggga tcgaaggcaa gcagtcgccg gaacgccgcc gctgcctcct
     1261 ggagccttcc cgcccgggcg gccgcgcgcg cgaaagcttc gtaggcgtga acgtaagtgg
     1321 gtgcggccct gagagcgcgg gcgaacagcg tggtcgcctc ctcgaagcat tgccgcgcca
     1381 gggccacgtt ggcaaggttg agctgcgcgt ccggccagtc cggccgtagg gcgagcgagc
     1441 ggtgtaacag gcgctccgct tcgtcaaggt cgccgcggtt gcgtcgcagc gtggcaagat
     1501 tcattaatgc ctcgggaaaa tccggccgca gcgagatggc cttcgcaaac gccgcctcag
     1561 cgtcgtcgat ccgcgcagca atcgtcagtg cgttgccgta gttcgcgtgg tattcgggca
     1621 cggcgggatt gagcgcgatc gcccgctcga acagcggcag cgcctcatcc ggccggttga
     1681 gctgggcagc caatagcccg agcaagtgat acgcgtccgg ctgatccggc tgccaccgca
     1741 gcgcctggcg gtacatctgc tcggcctcgg cgagccggcc gtcgcgatgc ctggccatcg
     1801 ccagcgccag gattcttcca aagacatcga tgcgtggtcc tcgcgcgctg agtcggcgca
     1861 tcggcatggg ccgcgccgca ggagcgcgcg aagtcaatgt cggctggacg aatcgcagca
     1921 agtgcaacga gcagtgggag gccaagggca cactattctt gcaaacgcgg acgcagccca
     1981 cagagaccgg actggctggc tgaggtggta ggactcgaac tcagaaatgt cgtcgcaaaa
     2041 tatcccgaaa gctcgcgcgg cccggctgcc cgcgaaagcg ctgcattggt cagttgatca
     2101 tgacgcgacc gtcacccggc gcgttgtgac agccctggcc tgccgagccg tatcccctcg
     2161 gcgggccggg gcgcttacgc gatccccgcc ttgcctgatt tgcgctttag gataacatcg
     2221 gagcgtctag ggcaccttga ttccctagat gtcttcatgg acatctcctt ggaaagcccc
     2281 ggcacgtccg gggctttctt ttactctctg tgcatcgtcg ggcgcgaacc ggcggcaggc
     2341 cccagcgtta gtcagttggt catctgcgcc tgccgaccaa ctcggagcag atgacaagtc
     2401 ccggcccgtc gttctgctta gcggcgggtc ggggcgctta ccgcgccttg cctaattcgt
     2461 gccttgggat agtatcgggg cgtctagagt tcgccctcta gatgtttcgt cacctcaagc
     2521 cccggcacgc ccggggcttt ctttgtgcgc cgagtacgaa cgatagcccc acttctctta
     2581 gtccactgtg aaaagctgcc accatgtggg ccgttccacc gggcgaaccg ctctggagga
     2641 acccgatcat cggcatcgcc ggctgctgcg cacgtgccgt gagcggccat gcaccagcgc
     2701 cgccgagcag cgtgatgaac tcgcgccgtt tcattgccag tgtttcccgt gcttccgatg
     2761 gggaagaata gcacagcact actgcactgc gggatttcaa tttgggctat gtccgctttg
     2821 ggtcaggagc ggacatcacc cgttcaccta gcaatgtccg ctttaccccc gaaagcggac
     2881 atggtgagct tgcctcgcga acctcgcggc ggctctcatt gcgggaagaa taggacattg
     2941 cgcgactcga tttcgctgcc ccaactcgta ttcttgacga gaacggagat cgccggcaac
     3001 gccgcgatct tttcgccgcg ctggacgatg aacgcctcgt agccgaagct ctcacagatc
     3061 gctgccacat cagcggcagg attctcatgc gtacctgaca atagctcgag catgattatc
     3121 ggccggtcgc gcgcgatagt tgctcgagcg ccgtcaagga cctcgcgctc gccgccctca
     3181 acgtcggcct ttacgaaacg cacgcccgcc aagccggctt cgtcgagcgt gcggatt
//
LOCUS       DATLJT010000830         1633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1431897,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000830 DATLJT010000000
VERSION     DATLJT010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431897"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..968)
                     /locus_tag="VM910_18800"
     CDS             complement(147..968)
                     /locus_tag="VM910_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI64609.1"
                     /translation="MSYATLMVYVDVEGKPDGRVSVAADLARRFDAHLIGIASGAPIS
                     FVLGEDAPNEAAAETPQLRDIKALFDEKAQQFRAAVGTNVRGLEWRSVVGLQADVVAR
                     EARAADLVIIGNVRASRDPFRALDPGSFILKAGRPVLVVPTGVTALPPRRVAVAWKDV
                     REARRAVQDALPFLREAENVVIVEVSERGGDQASSRLRNVANYLARHRIATVTERVLP
                     VDATAADSLLRFIADENISLVAAGGYGHSRLGEWAYGGVTRDLLAESPVCCLFSH"
     gene            complement(1035..1385)
                     /locus_tag="VM910_18805"
     CDS             complement(1035..1385)
                     /locus_tag="VM910_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64610.1"
                     /translation="MQSAVQIDFQGISRARPCDIAEHVAGLADRFGRITACRVVVKAP
                     SEHHRTGGLYEINIRLALPDGKEVDIGRTPRLDQRRADLHFALNDAFKRARRQLQDQV
                     RRLQGHVKTHPARP"
     gene            complement(1396..>1633)
                     /locus_tag="VM910_18810"
     CDS             complement(1396..>1633)
                     /locus_tag="VM910_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HVI64611.1"
                     /translation="GFIGALTEDEQVDLVALAWLGRGDGTLDDWEELRAEAARLHNKR
                     TASYLLGMPLLADHLEDALSQFGCSCEEFEMGHL"
BASE COUNT          282 a          540 c          510 g          301 t
ORIGIN      
        1 cgcgcgtcac ggtcgtgacg tcgcgcgtca tgtattggcc tgccgtgcat tggagaaatc
       61 ggtacatcag tccacccggc tcgttgtccg cttacgcgcg gttcggttgt cgtatggcgg
      121 aatcattttt cgatccttcg ctgccgtcaa tgcgaaaaca ggcaacagac cggactttcc
      181 gccaacaggt cgcgggtgac cccaccgtac gcccattccc ccagccgcga gtggccgtag
      241 ccgccggcgg ccaccaagct gatattttcg tccgcgatga accgcagcaa cgagtccgcc
      301 gcggttgcgt cgacaggcag cactcgctcg gtgaccgttg cgatgcgatg tcgggccagg
      361 tagttggcga cgtttcgcag ccgcgacgag gcctgatctc cgccgcgttc cgatacttcg
      421 acgatcacga cgttctcggc ctcgcgcaag aacggcagcg catcctggac ggcacgacgg
      481 gcctcgcgca catccttcca ggcgaccgcc actcgcctgg gcgggagtgc cgttacgcca
      541 gtcggcacga ctaaaacggg ccggccggcc ttgaggataa agctgccggg atcgagagcc
      601 cgaaacggat cgcggctcgc tcggacattg ccgatgatga cgagatcggc cgcccgcgcc
      661 tcgcgcgcga ccacatcggc ctgaagccca acgacggatc gccattccag tccgcgcacg
      721 ttcgtcccca cggcagcgcg aaactgctgt gctttttcat cgaacagggc cttgatgtcc
      781 cgcagttgcg gcgtctccgc agccgcctcg ttgggcgcgt cctcgccgag aacgaacgag
      841 atcggcgccc cgctcgcaat tccaatcagg tgggcgtcga agcggcgggc gagatcggcg
      901 gcaacgctga cgcggccgtc gggcttgccc tccacgtcga cgtacaccat cagcgtcgca
      961 taggacatgg ctcgcgatct ctaccaaatc cgcatgagat ttgaatttcc gtcgtcagag
     1021 gctcggatca agcgctacgg tcgagccggg tgcgttttga cgtggccttg caaccggcgg
     1081 acctgatcct ggagctgtcg acgtgcccgc ttgaatgcat cgttgagggc gaaatgaagg
     1141 tccgcacgcc gctggtcgag acgtggggtg cgtccaatat cgacctcctt tccgtccggg
     1201 agcgccaagc ggatgttgat ctcgtagagg ccgcccgtgc ggtggtgttc gctcggtgcc
     1261 ttcaccacca cccggcaggc ggtgatgcgc ccgaagcgat ccgcgaggcc ggcaacgtgc
     1321 tcggcaatat cgcaaggcct cgcgcggctg attccttgga agtcgatttg gacggcactt
     1381 tgcatgcgta ccgtcctaca gatgtcccat ttcgaactct tcgcacgagc agccgaactg
     1441 ggagagagca tcttccagat ggtcggccag cagcggcatt cccagcagat acgaggccgt
     1501 gcgcttgttg tgcaggcgtg cggcttccgc gcgcagttcc tcccagtcgt cgagcgtgcc
     1561 gtcgccgcga ccgagccagg ccagggccac gagatcgact tgctcatcct cggtcaacgc
     1621 cccgatgaag cct
//
LOCUS       DATLJT010000831         5866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_283918,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000831 DATLJT010000000
VERSION     DATLJT010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283918"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VM910_18815"
     CDS             complement(<1..437)
                     /locus_tag="VM910_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64612.1"
                     /translation="MTPSSTQHGGTVVALGGRRIDAEPTPTPRFPFDQVDRVGMEIAD
                     QLRRTQAVALVCSAACGADLIALETAQKMGLPTRIILPFSAARFRKTSVVDRPRPKFW
                     GSMFDRVTSAARADGNLVELDAPEADDAYSMTNGVIIREAKKLA"
     gene            complement(542..1864)
                     /locus_tag="VM910_18820"
     CDS             complement(542..1864)
                     /locus_tag="VM910_18820"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HVI64613.1"
                     /translation="MPSCFVIMGYGKKTDFKQNKNFDLDKTYRNIIKPAAQIAGYTCE
                     RADEIQHAGVIDVPMYERLLTADLVIADLSTANVNAFFELGVRYALRPKTTIVMAEKN
                     FEMGFDMGHVVIRKYEHLGPGIDYEEVERAKAELITACREVPQTGQIDSPVYTFLSDL
                     AAPSRGLNVKAVAAVAAAAERARDDRTREALAQATTQDEKAALERPFAALMKTARDAR
                     ARGDFRTERDVLRGVLAVQGDKADSDVVQQLAWATYKSKDPDEKTALLESKAVLGQLD
                     PEGSSDPETLGLWGAIHKRLYNTKDVPRQDRLDALAAAIFAHEKGFYLRNDYYNGINF
                     TFLLDCRAAELGGDDAIADRVQARRVRERVVGICTRLLAKGIKGENEDKRREEEYWVR
                     ATLVEALFGLGNTTKSEAEFAIARKAAPEQWMIDTTQEQLTKLRALQR"
     gene            complement(1980..3149)
                     /locus_tag="VM910_18825"
     CDS             complement(1980..3149)
                     /locus_tag="VM910_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI64614.1"
                     /translation="MPGRDSERRVDLTKRLARKGPRKLLALDGGGIRGVLSLHILGRI
                     EELLIKESKRPDYRLADYFDYVAGTSTGGIIAAGIALGMSVAEILDFYARNGAAMFDK
                     ASLMQRLRYEYNSEPLAKQLKSVFGEDTTLGAKELECLLLLVMRNATTDSPWPLCNNP
                     FAKYNDPAHPGCNTKLPLWQLIRASTAAPTYFPPEVITCGDKSFIFVDGGVTMYNNPT
                     FQMFLMATVDRYWIKAPPENRGWETGTDKMLIISVGTGTSPGENYSLKPNEMNLLFNA
                     TTVPSALMYAALNEQDLLCRVFGDCVAGHILDREVDKMSPSVGPLRQKLFRYIRYNAE
                     LTKQGLAALGCGDVDPEVVQKLDSVDGIPSLERVGKATAQRQVDATHFNFQVFQP"
     gene            3404..5107
                     /locus_tag="VM910_18830"
     CDS             3404..5107
                     /locus_tag="VM910_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI64615.1"
                     /translation="MIRRLRLASGLVMLAYVTTHFVNHSLGLVSLQVMDFALEHIYQY
                     WASPLGSVLLYGAFATHYSLALWALWLRRSLKMPLAEATQLVLGFSIPFFLTDHVLQT
                     RVADTFYGANVGYYSHVLHTYFVANPLRGGLQFAVLVIAWVHAMIGLWFWLRLKPWYP
                     RWQPILYAFALLIPTLAILGALEGGRQVMAMAEDPAWVAQQAQDHAPAAPADAAIIDQ
                     LVVWVRYAFVAALLAVLAARMARWYWQRRRGIVRLTYPNGRVVQIVRGVSVLEASRMA
                     GIPHASVCGGRGRCSTCRIRVDGAKEAIVPPAAEEVKVLHRVGAAPDVRLACQLRPHG
                     DLRVTPLLPASAQARDGFSRPGYLHGAEREIVILFGDLRSFTQLSEKKLPYDLVFLLN
                     RYFAETGHAVEAAGGRIDKFIGDGVMALFGLDSGVEAGCREALAAAKDMAERMETLNR
                     ALIHDIPEPLRIGIGIHTGPAIVGEMGYGTAVSITAVGDSVNTASRIEALTKTYACEL
                     VISEAVALRAGIDLGDAPRHEIEIRGRVERLVVRTFTSARDLPMLERRGRKKAASVAA
                     V"
     gene            complement(5544..>5866)
                     /locus_tag="VM910_18835"
     CDS             complement(5544..>5866)
                     /locus_tag="VM910_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64616.1"
                     /translation="TLLPLRGLPLGLVSLLALRLRLLVLLPLALARGLIALLLSLARL
                     LGLTLFAAILLRPLLMLTLGRLARGLLFLSALRLALLLALRALLRWPLHVVPALGLAA
                     RSGL"
BASE COUNT         1094 a         1813 c         1777 g         1182 t
ORIGIN      
        1 gccaatttct tcgcctcgcg gataatgacg ccgttcgtca tcgaataggc gtcatcggct
       61 tcgggagcat caagttcgac gaggttgcca tcggcgcggg cggcgcttgt cacgcgatca
      121 aacatgcttc cccaaaattt ggggcgcgga cggtccacga ccgatgtttt gcgaaagcgt
      181 gcggccgaga acggaagaat gatgcgcgta ggcagcccca tcttttgcgc agtctcgagg
      241 gcgatcagat cggcgccgca ggcagcggaa caaaccaatg caaccgcctg cgtgcggcgc
      301 agctggtcgg caatctccat tccgacgcgg tcgacctgat cgaagggaaa ccgtggtgtc
      361 ggcgttggtt ctgcatcgat ccgccggccg cccagagcga cgactgtgcc accgtgttga
      421 gtggaagacg gggtcatctc gtcacgctct caatcgctgg aacggttatc cgcgccccgg
      481 cgggtggata agcatcttga gttccgacgc gacacatggc ggaccggata cccgctcccc
      541 gtcatcgttg caaagcgcga agcttggtga gctgctcttg cgtggtatcg atcatccact
      601 gttcgggagc agccttcctg gcgattgcaa actccgcctc cgatttggtc gtgtttccca
      661 agccaaacag agcctcgacg agcgttgctc gaacccaata ttcttcttct ctccttttat
      721 cctcgttttc gcccttgatg ccttttgcga gcagtcgcgt gcaaattccg acgacgcgct
      781 cgcgcacccg tcgggcttgg actcggtcgg cgatggcgtc atcgccgccg agttcggcgg
      841 cgcggcaatc aagcaggaag gtaaagttga tgccgttgta ataatcgttc cttagataga
      901 agcccttttc atgagcaaaa atcgctgctg caagcgcgtc aagtcgatcc tgtcgcggca
      961 catctttcgt gttgtagagg cgcttgtgaa ttgcacccca cagacccagt gtttccggat
     1021 cgctagaccc ctcgggatca agctgcccaa ggactgcttt ggattcgagc aaggccgtct
     1081 tttcgtcggg atctttggac ttgtaggtcg cccacgccag ctgctgaacg acgtcggaat
     1141 cggccttgtc gccttggacg gcgagcacgc cgcgcaatac gtcacgctcg gtccgaaagt
     1201 ctccgcgcgc tctcgcatcg cgtgccgttt tcataagcgc cgcgaagggg cgttccaatg
     1261 cagccttttc gtcctgggtc gtggcctgag ccagggcctc gcgtgtgcga tcgtctcgcg
     1321 cgcgttcagc tgccgctgcg actgccgcca ctgctttgac attcaaaccg cgcgaaggag
     1381 ccgctagatc gctgaggaaa gtgtataccg ggctgtcaat ctgccctgtc tgaggaacct
     1441 cccgacaggc tgtgatcagc tccgccttcg cccgctcgac ctcctcgtaa tcgatcccag
     1501 ggccaagatg ctcgtatttt cgtatcacca cgtggcccat atcgaacccc atctcaaaat
     1561 tcttctcggc catgacgatc gttgtttttg ggcggagggc gtaccgcacg ccgagctcga
     1621 aaaatgcatt gacgttggcg gtagagagat ctgcgatgac caagtccgcc gtcaacaagc
     1681 gctcgtacat gggcacgtcg atcacgccgg cgtgttggat ctcgtccgcg cgttcgcagg
     1741 tgtacccggc tatttgagct gccggcttga tgatgtttcg ataagtctta tcgagatcga
     1801 aattcttgtt ctgcttgaaa tcggtcttct tgccgtagcc cataatcaca aagcaactgg
     1861 gcatggggtc ctcccgtctg gccaagggcc cgccgtttca gatctcaggc ccacgactag
     1921 ctgacatatc gatcaagacg ttgacgcggc gcattgcgat tgcggcgcat ccacggcgct
     1981 catggctgaa agacttgaaa gttaaagtgc gtagcatcga cttgccgttg ggccgtcgcc
     2041 tttccgacgc gctcgagcga cggaatacca tcgacagagt cgagcttttg gacgacttcc
     2101 ggatcgacgt caccgcaccc gagcgcggcc aggccctgtt tggtcagctc ggcgttgtaa
     2161 cggatatagc ggaacagttt ttgacgcagc ggtccgaccg acggactcat tttatcgacc
     2221 tcacggtcga ggatatgacc ggccacacag tcgccgaaca cgcggcacaa caaatcctgt
     2281 tcgttgagcg ctgcgtacat cagagccgac gggacggttg tcgcattgaa caggagattc
     2341 atctcgttcg gtttgaggct gtagttctcg cctggactag ttcccgtccc cacggagatg
     2401 atcagcattt tatccgtgcc cgtctcccag ccgcggtttt ccggcggcgc cttgatccag
     2461 tagcgatcca ccgtcgccat cagaaacatc tgaaatgtcg gattgttgta catggtcacg
     2521 cccccgtcga caaaaatgaa cgatttgtcg ccacacgtaa tgacttccgg gggaaaatat
     2581 gtcggcgcag ccgtgctcgc ccgaataagc tgccacaacg gtagcttcgt gttgcatcca
     2641 gggtgcgctg ggtcgttata cttcgcaaac ggattgttgc aaagcggcca cggcgaatcg
     2701 gtcgtcgcgt ttcgcatcac cagaagcagc aggcattcga gctcttttgc tccgagcgtc
     2761 gtatcctcgc cgaatacgct ctttagctgt ttcgcgagcg gctcgctgtt gtattcgtaa
     2821 cgcagccgct gcatgaggct ggccttgtca aacatggcgg caccgtttct cgcataaaaa
     2881 tccaggattt cggcaaccga catgcccagc gcgatacccg cggcgatgat gccaccggtg
     2941 ctcgtgcctg ccacgtaatc aaagtaatca gcgagccggt aatccggcct cttgctttcc
     3001 ttgataagaa gctcctcgat ccggccaagg atatggagcg acagcacacc gcgaatgcct
     3061 cccccatcaa gggccagcag cttacggggt ccttttcttg cgaggcgctt tgtcagatca
     3121 acgcggcgtt cggaatcgcg accgggcatg ttttgcctac cggtgattgg cccatgttag
     3181 aggcctgctc gtgaacccgt ctgtgtcgac ttatatcgca aattgggtgg gagccatcgt
     3241 gcttaccctt gggctgccgg aaagaacaat atcatcgagc acctggcgtg tcggtggggg
     3301 cggtgaccag cgcatcattc cggaaacaga gcttttatac ttcgcatccg caggtcatga
     3361 tgtaagtcgc gaacgcgtgt gacgacgggc gcgggtggaa ggcatgatcc gacgactgcg
     3421 gttggcttcc ggactcgtca tgttggcata cgtgacgacc cacttcgtca atcacagcct
     3481 gggcctcgtc tcgctccagg tcatggattt cgcgcttgag cacatctacc agtattgggc
     3541 gtctccgctc ggcagcgtcc tcctctacgg cgcgtttgcg acccactaca gcttggcgct
     3601 ctgggcgctc tggctgcgcc gttcgctcaa gatgccgttg gcggaagcaa cccagctcgt
     3661 cctcggattc tcgattccgt tttttctcac ggatcacgtc cttcagacgc gcgttgccga
     3721 caccttctac ggcgctaatg tcggctatta ctcgcatgtg ctgcacacct atttcgtggc
     3781 caatccactg cgtggcgggc ttcagttcgc ggtgctggtg atcgcttggg tccacgcaat
     3841 gatcggacta tggttctggc tgcgcctgaa gccgtggtat ccacgctggc agccgatcct
     3901 ctacgcgttc gcgctcttga taccgacctt ggcgattctt ggcgccctcg agggcggccg
     3961 gcaggtcatg gcgatggccg aggatccggc ctgggtcgcc cagcaggcgc aggaccatgc
     4021 gcccgcagca ccggccgacg ccgcgatcat cgatcagctg gtggtgtggg tgcgttacgc
     4081 cttcgtggcg gcgctccttg ccgttctggc ggcgcggatg gcgcgttggt actggcagcg
     4141 ccggcgcggc atcgtccggc tgacttatcc gaacggccgc gtggtgcaga tcgtgcgcgg
     4201 cgtgtcggtc ttggaggcga gccggatggc tggcattccc catgcctccg tgtgcggcgg
     4261 gcgggggcgc tgctccacct gccgcatcag ggtcgacgga gcgaaggagg cgatcgtgcc
     4321 gcccgcggcc gaagaggtga aggtgttgca tcgcgtgggc gcagcgcccg acgtgcggct
     4381 cgcctgtcag ctgcgcccgc acggcgatct gcgcgtgacg ccgctgctgc cggcgagcgc
     4441 ccaggctcgg gacggcttca gccgtcctgg ctatctccac ggcgccgagc gcgagatcgt
     4501 gatcctgttc ggcgatctca ggtctttcac ccagctctcc gaaaagaagc tgccctatga
     4561 cttggtattc ctcctcaatc gatactttgc cgagaccggc cacgcggtgg aggcagcggg
     4621 cgggcgcatc gacaagttca tcggcgatgg ggtgatggcg ctgtttggtc tcgacagcgg
     4681 cgtcgaggcg ggctgccgcg aggcgctcgc cgcggccaag gacatggcag agcgcatgga
     4741 aaccctcaat cgcgcgctga ttcacgacat acccgagccg ctgcgcatcg gcatcggcat
     4801 ccataccggc cccgccatcg tgggcgaaat gggttacggc accgcagtct cgatcaccgc
     4861 ggtcggcgac tccgtcaaca cggcgagccg catcgaagcc ctcaccaaga cctacgcctg
     4921 cgagctcgtg atctccgaag ccgtggcgct gcgcgccggg atcgatctcg gcgatgctcc
     4981 ccgtcacgag atcgagatcc gtggccgagt cgagcggctc gtggtgcgca catttacgag
     5041 cgcgcgggat ctgcccatgc tggagcgtcg aggacggaag aaggccgcga gcgtggccgc
     5101 agtgtagccg tgtaaagcca gccgttcgtc ggctccgcca gttataaccg ccaaccagat
     5161 cgcttccctc gcgccacgct ggccgacatc gttagcaatc ggcagtgcgg gtccgcgtgc
     5221 ccgcttcccc gcgtgccagc ctaaaggcgt tgtcggtgcc ctcaacctcg cgcgagagca
     5281 aagcaaacac cattaaagcc cgcgtgaaag ttcccctggc cgtgcttatg cggtcactcc
     5341 cacatcgcgt ggggaaccac acgaaagctt cttcgtttta ccccgggcca agcgttcggc
     5401 gcgcggggaa caacgagaga ggaaccgttc caatgaggaa caggcttttg atcagtacgg
     5461 ctgcgctatt ggcgggagtc gccttggcgt cagcccaaaa catgccaggc ggaggagagc
     5521 aacgcggcgg tgctgcgcaa caatcacagt ccgctccggg cagcgagacc cagcgcgggc
     5581 acgacatgca agggccatcg cagcaacgca cgcaaggcca gaagcagggc caatcgcagc
     5641 gcggacaaaa agaggagacc acgggccaag cgccccagag tcagcatgag cagggggcgc
     5701 agaaggatcg cggcaaacag ggtcaatccc agcagcctag ccagggacaa cagcagcgcg
     5761 atcagaccac gggccagagc cagcggcagc aagaccagca ggcgcagccg cagcgccagc
     5821 aacgagacca gacccaaggg caggccccgc aacggcagca gggtca
//
LOCUS       DATLJT010000832        20415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1202439,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000832 DATLJT010000000
VERSION     DATLJT010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20415
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202439"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VM910_18840"
     CDS             <1..760
                     /locus_tag="VM910_18840"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVI64617.1"
                     /translation="AHIHVRDKRGVTTSDLSVYSEVVDGIMSNCPGMITQVGNGIGLA
                     YHDPKKEGQPFTLEERMRLLEIQPTPDQLTLNLGTFHFEHKNSEFLFPNSKRWNTEFV
                     KDCRTRNIDNELEVYDLSHIQNALDLVERGELKPPLHFSFVLGIRGGIPASPANLLAM
                     LDAIPEGSTWQIVSIGKYQLPLSTMALAMGGNIRVGMEDNVYYSHGVLAVSNAQLVER
                     AVRIAGELGREIATPEEARKMLHLRPTPQRAPAQ"
     gene            complement(830..1600)
                     /locus_tag="VM910_18845"
     CDS             complement(830..1600)
                     /locus_tag="VM910_18845"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64618.1"
                     /translation="MAILSAHRVSKEYPGHSRSDPPVLALRGVNFTVDHNEFCSVLGH
                     SGCGKTTLLTLVAGFEEATTGQILLDGKPVGKPGWERSMIFQDYALFHWLTVEDNIAF
                     GLQMKNVPRHKQKSMVNDYVNLVGLSGFERRYPHQLSGGMRQRVSIARALAVNPQILL
                     MDEPFAALDAQNRSMMQQEMVRIWERARKTCLLVTHSIEEAITLSDHILVMTRRPGTI
                     KSDLRVDLPRPREEGDPKVIALKTKIRELIHDEFDIER"
     gene            complement(1622..2431)
                     /locus_tag="VM910_18850"
     CDS             complement(1622..2431)
                     /locus_tag="VM910_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64619.1"
                     /translation="MTAGTGKPATASSAWLQRFKRLRALGLLAIVLAIWQVVSTFVLD
                     PSTAMLLPAPTAVAKAFWELILSGELLSHLRDSLKREIVAFLWATSAIPLGIAMGWSK
                     VVNEQVDPLVEVLRPVPPLAWIPLSILWFGIGDVQNQFIIFLGIFFPVLLNTIMGVRN
                     IEPNLVRAARCLGASEIRVLRRIVLPAALPQIVTGIRVGLGVGWMALVAAELVGANSG
                     LGFLINDARTLLRTDIVIVGMITIGVVGLLIDRTIRVSGRRLMPWSRTFSA"
     gene            complement(2507..3790)
                     /locus_tag="VM910_18855"
     CDS             complement(2507..3790)
                     /locus_tag="VM910_18855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64620.1"
                     /translation="MTVSCIARRELLLGGISLAGLLTIHGTARAQGAQFTEWGWRRPY
                     EKISDKSVVWLKEKGWWPLQIAFQAPWSGQNNINIVMDKAGLLAQRGIEAKLQAFGSG
                     PAINEVLVSAKFQVASSGNFPFTSLLDKEIPVKVIALITPNILHATIVSKDSKITSLK
                     DFKGSNPPATIGIVTGSSAEFYFQMAATVHGLQIGKDVILKNMPIGEQMALPKGIDAV
                     VPWDAGATMMVEERKNGKVIDTIYPYNMYEGNFVVRQELIDNVADVVQALSDAYVEAL
                     LWSRLYLDKTVQLLQDDPSLKNFTKGILTQQTRVYNSLYKPTFVYPHAKFWGEANAPI
                     FKWLYQFKRIQRPLTATDFERSVDPRFIDKTFDKLGWVVPKTPPFIPANWTGSLDKLP
                     YPEYLTPVNLKAPQPFPEKGDLTKPWSFGGQTFNP"
     gene            complement(4373..5248)
                     /locus_tag="VM910_18860"
     CDS             complement(4373..5248)
                     /locus_tag="VM910_18860"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HVI64621.1"
                     /translation="MYAGKLRPIGSRKRQHDHVTQGNKIKMSFLNLQNRTSIVTGATR
                     GIGRAICEELAKAGSDIALFDIDDEKMEQFARKLTGEFGISTRVYKLDVRRSADLCSR
                     AWSVVDEFGGIDHLINAHGVQFLSPFAEFPEDKWTFIHDVNLMGVFLASKAVWPHMVK
                     RKRGRIVNVASVHGIVASELKSAYIASKHGVVGLTRATALEGAEHGITVNAVCPGAVL
                     TDLVRQQGPQYAKRLGPNVSEQQALNRAFLDTMPTRRFIDPSEIGQLCTYLCSDAARS
                     ITGSAIAIDGGWSAR"
     gene            complement(5604..6599)
                     /gene="pta"
                     /locus_tag="VM910_18865"
     CDS             complement(5604..6599)
                     /gene="pta"
                     /locus_tag="VM910_18865"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HVI64622.1"
                     /translation="MDLLGGLFDSARRHLRKVVLPEGDDARVLAAASRLRNEKIAVPV
                     LLGSEEVIAQRAVQAELDVKGLEIIDPHRAAGLESYAAAFVTSRRTMTPGMAKRLVTK
                     PLYFAGMMVHQGDADAMVAGAANPTRRVIEAGLMTVGLADGIALPSSFFLMMVPDFLG
                     RGPKTLIFADCAVNADPTAEELADIAVASACSAERLLGESARVAMLSFSTHGSAHHAR
                     VDKVRRAVALARERAPRLAVDGELQADAALVPAVAQRKVKDPSATAGRANVLVFPDLD
                     SANIGYKLTQWLGGAKAVGPFLQGFSKPISDLSRGASVDDIVTTCAVVLARGYSR"
     gene            6642..7784
                     /locus_tag="VM910_18870"
     CDS             6642..7784
                     /locus_tag="VM910_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVI64623.1"
                     /translation="MTEISEPIKIHRGLKGVYFDRSPCTFIDGKAGELRYRGYSIHDL
                     AENSTFEETAWLLLNGELPNAQQLSAFDKDLKAARSLPEPIYEIIHVMKSAHPMDVLR
                     TAVSALSAFDPDVADNSREATLRKGIRLTSQVAMIVAAHSRIREALEPIPADRELGHA
                     ANLLCMLKGTKPSRDAAQLIDRDLILHAEHGSNASSFTARVVVGTEANLHAAITAAIA
                     ALSGPAHGGAAEDVMKMAEEIGDAARAADYVRAKRKAGEPVTGFGHRVYRAEDPRARH
                     MRAGVEKLSKEMGEPRWYEILQAVVQAMAPYARHGVNVNVDFYSGVVYHLLGIKRDLF
                     VPIFAVGRVPGWVVQVLEQMGNNILIRPLTLYSGPELRSYGSISTR"
     gene            7932..8888
                     /locus_tag="VM910_18875"
     CDS             7932..8888
                     /locus_tag="VM910_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64624.1"
                     /translation="MHVARKLLLWSVVACVGLTLAAIPARAEYPERPIELIVPWGPGG
                     GADQLGRLVSKLAEPLLGQGIPVVNVPGATGGTGMAKLLSAPADGYSAAIYIADSHAL
                     LAGKNPRWSMDDIAPVAVMIQGPSFIFVPQDSHFKTWQDFEKEAKANPGRLKIATLGF
                     ASVDDFSISVIERSKGLKFVQVPFSKPGERYVSILGGHADALYEQAGDVASFLNSKQM
                     RPLLIFGKERLPAFKDTPSSYELGIPVALPQFRAIVVRSGTPPAIVHKLSETLAKVAQ
                     TDEYKKFLVDQFGDANSFKDASTAPAFVSQQLDDMKKAMAAN"
     gene            8922..9464
                     /locus_tag="VM910_18880"
     CDS             8922..9464
                     /locus_tag="VM910_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI64625.1"
                     /translation="MDKALFRSIGPYVLGLAIAVALYIYAGTFEYTPRAGALGPNVWP
                     CLAILLMGASCLFELTRRLIIGNKDVVGFMEALDREPEAEESQPAYPRRVIGGVVLMG
                     VYAMLVPILGFVLGTFLFLAAFMYIGGYRAHGVIWGASATVTIFCGILFLRIAYVSLP
                     RGIEPFDRVTDGFFAIPSMW"
     gene            9469..10971
                     /locus_tag="VM910_18885"
     CDS             9469..10971
                     /locus_tag="VM910_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI64626.1"
                     /translation="MIDIITQLAAGFVHCLTPLNIAMLLIGIVIGLLIGVLPGLTLVM
                     GVTLALPFTYKMEITASIVLLTAMYVSGTYGGAFTAILFRIPGEPIDVPMLWDGYTMG
                     RKGRPAKALGWTLVAALGGGLLSAVVMVALTQPLASFALRFSSPEYFVIVMFGLLSVV
                     AIGKGSLANAFISMSVGILISSVGVDPVYGAYRFTFGLPILADGIEFLVVMVGAYGVG
                     EVLTRLDTGFATKPLDKISNARTELPTLKELNEIKGMFLRSSVVGDLIGLLPGAGATI
                     ASFVSYGIEARFGKRRDQMGTGVAEGIVAPQSAATASVGGAMVHLLALGIPGSGATAV
                     ILGAFMLHGIQPGPQVLVNSASMVYTVFASLFLGLALMCAIGYFAIRPLVKILDLPEA
                     VVSAYVLILCFIGALSIRNNVTDLWLIIGFGVLGYGFERLKFPIAPLVLGVILGPIAE
                     EAFMNTMISFSNDWTVFFTRPISGTIVALTIIVLLLPFVQHLIRRRFATA"
     gene            10992..11864
                     /locus_tag="VM910_18890"
     CDS             10992..11864
                     /locus_tag="VM910_18890"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVI64627.1"
                     /translation="MRSSLNLGARAVQSAGARGRVAELRPLTPAGKSATARTHKLGAT
                     ARSLAILEHVASSQAPVDVLDIISSLKLPKATAYRRVEWFITQGYLSREPGRRRLIVG
                     PKLANLAFGALSTAIRNGASHLVLQRLVRTLDETCNIGTLLNGEVIYLDRVEAEHWPL
                     RLHYTNGSRVPLHCTAIGKLFLALAATPHRQRLLQSVELRRFTDSTITDKARLETELR
                     QIRKERVSFDREEYLVGVVCMAVPVVGKNGEMLAALAIQAPKARMDVNTARAHLPALR
                     CAASELGEIFQDRG"
     gene            11947..13722
                     /gene="xsc"
                     /locus_tag="VM910_18895"
     CDS             11947..13722
                     /gene="xsc"
                     /locus_tag="VM910_18895"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HVI64628.1"
                     /translation="MKMTTEEAFVKVLQMHGIEHAFGIIGSAFMPISDLFPQAGITFW
                     DVAHEANGALICDGFTRSTGKIAMVIAQNGPGITNFVTAIKTAYWNHTPMLLVTPQAA
                     NRTIGQGGFQEVEQMALFRDMVGYQEEVRDPARVAEVLNRVISKAKRLSAPAQINIPR
                     DYWTQVIDISLPAIIEFERPAGGGDAVAEAAKLLSAAKFPVILSGAGVVLGNAIPECV
                     ALAEKLDAPVCNNYQHNDSFPGSHRLAVGPLGYNGSKAAMELVAKADVVLALGTRLNP
                     FSTLPGYGIDYWPRNAKIIQVDINADRIGLTKPVAVGICGDAKLVAKNILAQLSPSAG
                     NAGREERKALIHTTKSKWLQTLSSMDHEEDDPGTTWNADVRKREPDRMSPRQAWRAIQ
                     AGLPKDAIISSDIGNNCAIGNAYPTFDQARKYLAPGLFGPCGYGFPAIIGSKIGNPNT
                     PCVGFAGDGAFGISMNEMSSIGRAEWPGITMVVFRNYQWGAEKRNSILWFDNNFVGTE
                     LNPHLSYAKVAEACGLKGVTARTQAELTEALRESCEAQKNRVTTFIEVILNQELGEPF
                     RRDAMKKPVVVAGIRREDTRAQQSA"
     gene            13735..15249
                     /locus_tag="VM910_18900"
     CDS             13735..15249
                     /locus_tag="VM910_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI64629.1"
                     /translation="MSQEARSRRNPGAGRDIREGPAGKFRSSMTMPAPMKTPVDPSAN
                     AVVDQLVQRGRAAMAEFADADQGRVDEAVTALAWSLYNPAHARELAELAVADTKLGNV
                     ADKIIKKQRKTFGTLRDLLRVKSVGIIEDDAKRGIVKYAKPVGVVCAVTPSTNPGATP
                     VNKAMMAIKGRNAIIIAASPAGLKTTMRTVDYMRAELKKIGLPEDLVQILPPPASKDM
                     TEALMRAADLVVATGSLDNVRRAYSSGTPAIGVGTGNVPVIIDETADLDDAAKKICAS
                     KIFDNSTSCSSENSVIIVDAVYDQAMAALERAGGYRASAIEKERIQRALWPDGKLSRI
                     LIARDADVFARGCGLPAATEGKKFFMVEETGVGPDSPFSGEKLSLVLTVYRAKDFDAA
                     TEQANAILTFQGRGHSVGLHTKDPKRGRALAERLDVVRVLINQAHTFGNGGSFDNGLN
                     FTLSMGCGTWGGNSITDNLNYKNFLNITHLVTAIPEDKPSEQELFGAYWAKYGR"
     gene            15258..16385
                     /locus_tag="VM910_18905"
     CDS             15258..16385
                     /locus_tag="VM910_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVI64630.1"
                     /translation="MNFEEYVAKSLILAPAGIAVPRGQLCTTPDEVAVAVRSIGPAVI
                     KAQVATGKRGKAGGIRFVSTPPEAERVASAVLGMTIGEYRVERLLVEEQAKVAREFYA
                     AVLNDAQERKPLILFATEGGMDIEEIAAANPDAIRRLPIDIDATPSAADIARILEGSG
                     LGNAEASVADILSRLYAAYRQRDAELLEINPLAQLADGRVVALDAKFVLDDSAAYRQE
                     EIAKLAALAPMTALERLAAVHGLKFIQLDGNVGVLANGAGLTMTTMDVIRHYGGRPAN
                     FLEIGGEAYTKSEIALDLVLSNPGIKSLVINFCGAFARTDVMADGVVKAWKELKPSIP
                     VFFSIHGTGEEEAIALVRESLRIEPYKSMEDAIQAAVRVAQ"
     gene            16382..17257
                     /locus_tag="VM910_18910"
     CDS             16382..17257
                     /locus_tag="VM910_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA synthetase subunit alpha"
                     /protein_id="HVI64631.1"
                     /translation="MIVRKSDKVIVLGITGRQGTFWTDKMLAYGTQVVAGVNPKRAGE
                     YHCNVPIFASTADAVRETDGSVAVMFIPASVARHAAIAAIESRIRLLVVLTEHIPAQD
                     VMAVLAAAARHGTRVVGPNTAGLVTPGECFVGIMPAFVPSVFKPGRVGVISRSGSLGT
                     LICLKLTQAGVGQSAFVGIGGDPMLGTTSRDALEALDRDPATDAVVIVGEIGGTMEEE
                     AAEYAKTMNKPIAAFIAGAASPPGKKMGHAGAIVLGNRGSYASKRKALEAVGVIVVDT
                     PAEIVPALAIRDLGK"
     gene            complement(17498..18313)
                     /locus_tag="VM910_18915"
     CDS             complement(17498..18313)
                     /locus_tag="VM910_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI64632.1"
                     /translation="MIIAQISDTHLTLDTPDADQRIRDFALTIADINALDPAPDVIVH
                     TGDIVHNGRQDEYAQAVATLAKARAPVYVLGGNRDNRANLRAAFSASGYLAPDSDFID
                     YAIDDYPVRLIALDTLSSRSNKGDFCLERVRRLIELIDAETTKPIAVFTHHPPFEVSV
                     GPDRLHFETTETASRLGRALQHSGRVVAVFSGHVHRAAAGHVGRIPATVVQCIATTLR
                     KGEYPPHMKVRPVYQVHRFDPIWGFASETRIVEAQPMAPGICDSILPRRKRSP"
     gene            complement(18310..19506)
                     /gene="sat"
                     /locus_tag="VM910_18920"
     CDS             complement(18310..19506)
                     /gene="sat"
                     /locus_tag="VM910_18920"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HVI64633.1"
                     /translation="MSQLVPPHNSEQVKPLLLLETERAEERKRAEKFKKIPLDSRTIS
                     DVFMLAMGAYTPLDGFMGHDDWRGSCLDMKLTSGVFWPIPITLSVNKDLADSLHAEQE
                     VTLIDAETSEILAVMKIHEKYSIDKQLEAEHVYRTADPKHPGVAKVLHQGDVNLAGPI
                     MVLSEGEYPAKYPDLYIRPARSRAMFLERGWSNVAAFQTRNPMHRSHEHLVKIAVEVS
                     DGVFIHQVLGKLKAGDIPADVRTRAIQAMIDNYFRAGTVIQAGYPIEMRYAGPREALL
                     HALIRQNFGCSHLIVGRDHAGVGDYYGPFDAHHIFDQLWPGALHTKPLKIDITFYCRK
                     CYGMATAKTCPHDPQHHISISGTEQREMLSSNADIPPEFSRPEVVAILKEYYAGLRST
                     GQSPAS"
     gene            complement(19647..>20415)
                     /locus_tag="VM910_18925"
     CDS             complement(19647..>20415)
                     /locus_tag="VM910_18925"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HVI64634.1"
                     /translation="INPELTTSEPYVMGSHATCSGAWASGPEDYAPSEYQWGYNRMMT
                     VEGLFGAGDAIGGSAHKFSSGSFTEGRIAGKAAVRYVNEFGKQPPQVSETEYEDLRKA
                     IYKPLENYQVGRNEIVAGTVSPSYILPIHGLQRLEKIMDEYVGGIGAHYTTNEPLLAR
                     GLELLGMLKEDLAHLGAEDLHQLQRAWELQHRVLASECVTQHTLFRTETRWPGYYYRA
                     DHPKLDDKDWHCFTLSRYDRDSGKWEMQKAPVYHIVD"
BASE COUNT         4097 a         6203 c         6054 g         4061 t
ORIGIN      
        1 ggcccatatt cacgtgcgcg acaagcgcgg ggtaaccacg tccgacctct cggtctacag
       61 cgaggtcgtc gacggaatta tgagtaattg ccccggtatg ataacgcagg tgggaaacgg
      121 gatcggcctg gcctaccacg atccgaagaa ggagggccaa ccgttcacgc ttgaggagcg
      181 catgcgtctg ctggaaatcc agccgacacc tgaccagctc acgctcaacc ttggaacctt
      241 ccatttcgag cacaagaaca gcgaattcct gttcccaaac tcaaagcggt ggaataccga
      301 gttcgtaaag gattgccgca cgcgaaatat tgacaacgaa ctcgaggtct acgatctcag
      361 ccacatccaa aacgcactcg acctggtgga gcggggcgag ctcaagccgc cacttcattt
      421 cagcttcgtg ctcggcatcc gcggcgggat tcccgcctcg ccagccaact tgctagcgat
      481 gctcgacgcg attcccgagg gttccacgtg gcaaatcgtc agcatcggca aatatcagct
      541 tccattgagt acgatggcac tcgccatggg cggcaatatc cgcgtcggca tggaagacaa
      601 cgtctattac agtcacggag tacttgccgt gagcaatgcg caactggtgg agcgtgcggt
      661 gcgcatcgcg ggcgagctgg gacgagaaat cgcgacaccg gaagaagcgc gtaagatgct
      721 acacctgcgc ccgacgccgc agagggctcc tgcacagtaa ggcgcggcgg acgcacttat
      781 gcgatccggg cgatgacggg gagctcccgc aaggactaca gtcggttcct caacgctcga
      841 tatcgaactc atcgtggatg agctcgcgga tctttgtctt cagcgcgatg actttggggt
      901 cgccttcctc ccgcgggcgc ggaagatcga cgcgcaagtc gcttttgatc gtgccgggcc
      961 ttcgggtcat caccagaatg tggtcggaaa gcgtgatagc ctcttcgata ctgtgcgtga
     1021 caagaagaca ggtttttctc gctcgctccc agatccgaac catctcctgc tgcatcatgc
     1081 ttcggttctg tgcgtcaagc gcagcaaaag gctcgtccat caggagaatt tgcggattga
     1141 cggcgagagc gcgcgcgata gaaacgcgtt gccgcatacc gcccgacagc tgatgcgggt
     1201 agcggcgctc gaaacccgac aagccgacga gattgacgta gtcgttgacc atactttttt
     1261 gcttgtgtcg cggcacattc ttcatttgca agccgaaggc gatgttgtcc tcgacggtaa
     1321 gccaatggaa aagcgcgtag tcctggaaga tcatggagcg ctcccagccc ggcttaccca
     1381 ccggcttgcc atcgagcagg atttggcccg tcgtcgcctc ctcaaagcca gcgaccaagg
     1441 tcaagagcgt ggtcttgccg cagccggagt gacccagtac cgaacagaac tcattgtgat
     1501 cgacagtgaa attcaccccg cggagcgcca gcaccggcgg atcactgcga ctgtgccccg
     1561 gatattcctt gctcacgcgg tgggcggaga gaatggccat ggttttggtc ggccgttgcc
     1621 gctaggcgga aaaggtgcgg gaccacggca tgagtcgcct gccgctgacg cggatcgtgc
     1681 ggtcgatcaa gagccctact acgccgatcg tgatcatgcc aacgataaca atgtccgtac
     1741 gcaataaggt ccgcgcgtcg ttgatgagaa atcccagccc cgaattggcg cctaccagtt
     1801 cagcagccac cagggccatc cagcccaccc ccaagcccac acgaatcccc gtgacgatct
     1861 gtggaagcgc tgccggcaga acgatgcggc ggagaacgcg gatctcactt gctcccaggc
     1921 atcgcgctgc gcgcacgaga ttgggctcaa tattcctcac ccccatgatg gtattgagca
     1981 gcacgggaaa gaagatgccg aggaaaatga tgaactgatt ctggacgtcg ccgatgccga
     2041 accaaagtat cgagagcgga atccaggcga gtggcggcac cggccgcaac acctcgacca
     2101 gaggatcgac ctgttcgttg accaccttcg accagcccat ggcgatgccc aggggaatgg
     2161 cggaagtggc ccagagaaac gcaacaatct cgcgcttgag actatcgcgc aagtgactca
     2221 agagctcgcc ggacagaatg agctcccaga aggcttttgc gaccgccgtt ggcgccggca
     2281 gaagcatggc cgttgaaggg tcaagcacga acgtgctcac gacctgccag atcgcgagca
     2341 cgattgcgag tagacctaag gccctgaggc gtttgaaacg ttgcagccac gctgatgagg
     2401 ctgtggccgg cttgccagtt ccagcggtca cgaccgtgct cataatccgg cgtcttcacc
     2461 gaggttggcg ggcctgcctt tcgcggcggc gtgtccggtc atcgcgctac gggttgaacg
     2521 tttggcctcc gaatgaccac ggcttcgtca aatcgccttt ctccgggaat ggctgcggcg
     2581 ctttaagatt gaccggcgta aggtattctg gatacggcaa cttgtcgagc gaacccgtcc
     2641 agttggccgg aatgaagggc ggagttttcg gcaccaccca gccgagcttg tcgaacgtct
     2701 tgtctatgaa cctggggtcg accgatctct cgaaatccgt cgctgtaagc ggacgttgga
     2761 tccgcttgaa ctggtagagc cacttgaaga taggggcgtt cgcctcgccc cagaatttcg
     2821 cgtgcgggta gacgaaggtt ggtttgtaca gggaattgta tacccgcgtc tgctgcgtga
     2881 ggatgccctt ggtgaagttc ttgaggctgg gatcgtcctg cagcagctgc actgttttgt
     2941 cgagatacag cctcgaccag agcagcgcct cgacataggc gtcgcttagc gcctggacaa
     3001 cgtcagctac attgtcgatg agttcctggc ggacgacgaa attgccttcg tacatgttgt
     3061 agggatagat cgtgtcgatc accttcccgt tcttgcgctc ttccaccatc atggtcgcgc
     3121 cggcgtccca cggcacgact gcatcgatcc ccttcggcaa ggccatctgc tcgccgatcg
     3181 gcatgttctt caggatcaca tcctttccga tctgtaggcc gtgcaccgtc gcggccatct
     3241 ggaagtagaa ttcagccgaa gagccggtga cgatgccgat tgttgccggc gggtttgagc
     3301 ctttgaaatc cttcaggctc gttatcttgc tgtctttgga tacgatcgtg gcatgaagaa
     3361 tattcggcgt gatcagcgcg atcaccttta ccggtatctc cttgtcgagg agcgaggtga
     3421 aaggaaagtt gccgctgctg gcgacctgga atttggccga caccagcact tcgttgatcg
     3481 ccggcccgga gccgaaggcc tggagttttg cctcgatgcc gcgttgggcg agcagccccg
     3541 ccttgtccat cacaatgttg atgttgttct ggcctgacca cggtgcctgg aaggcgatct
     3601 gcagcggcca ccaacccttc tccttaagcc atacaaccga cttgtcggag atcttttcat
     3661 agggccgccg ccaaccccac tcggtgaatt gcgcgccctg cgcccgcgcc gtcccgtgaa
     3721 tggtcaacag ccctgcgaga ctgataccgc caagtagaag ctctctccta gcgatgcaag
     3781 aaaccgtcat tgctgttcct cccaccgtca attatctcgg ccgcagccga gatagaagtc
     3841 tcacatcaca gcagctgcct ttgcgatgtg attgtttcca ttgaataaag cgtatgaggg
     3901 gttccgagaa taggtcaagg ccaactgtcc cagcaactga gacgctgtcg ccaaaattaa
     3961 tcgtggtcga tccgctcttg tctgaagcga cccccggtaa atgtcacctt tggtcgaggc
     4021 cctcgtagga ggggagttcg tctcgcttgt gtgctggcca acccgctgca gatggccaag
     4081 atctttgcgg cggaggcccg gatggcgttg gcttcgggca acgcgcccgg ccgcaacgag
     4141 gcgctcggca cgggtaacaa gcgctgcaac agcttcgccg tcgatcgcgc caagcgccca
     4201 gtctcaacaa cgttcagcaa gttgaggcgg aaatcttccg cctcggggcc aagccatggt
     4261 cctctgaatc aatcaagagg ctcaagactc gccagtgggg gactctccag cctcccagtt
     4321 cagcccaaga tgaaaagcgc tgacgatcta caaagatcta cttgcagcta gtctaacgcg
     4381 cggaccatcc tccatcgatc gcaattgccg aacctgtaat ggaccgagcg gcatccgagc
     4441 atagataggt gcagagctga cctatttcgc tggggtcaat aaagcgccgc gttggcatcg
     4501 tgtcgaggaa cgcacggttc agtgcctgtt gctcagaaac attcgggcct agtcgcttgg
     4561 catactgcgg cccttgctgg cgaacgaggt ctgttaatac cgctcctgga caaactgcat
     4621 tgactgtaat cccatgctca gcgccttcga gcgccgtcgc gcgcgtcaat ccaacaacac
     4681 cgtgctttga ggcgatatat gcagacttca gttccgaggc cacgattccg tgcacgctcg
     4741 caacattgac gatgcgcccc ctctttcgtt tcaccatgtg aggccagaca gccttactcg
     4801 ccaggaagac acccattagg tttacgtcat gaatgaacgt ccatttgtct tccgggaact
     4861 ccgcaaatgg actgaggaac tgtacaccat gcgcgttgat gagatggtct atgcctccga
     4921 attcatcgac gaccgaccaa gcacggctgc ataagtcggc agaacggcga acatcgagct
     4981 tgtaaactct tgtgctgata ccgaactcac cggtaagctt acgagcgaac tgctccattt
     5041 tttcatcatc gatgtcaaag agtgcgatgt ctgatcccgc tttagccaat tcctcacaaa
     5101 tcgctcgtcc gatgccacga gtcgctccag tcacgatact ggtccgattt tgcaggttca
     5161 ggaaagacat ttttatctta tttccctgag ttacatggtc gtgttgccgt ttgcgcgatc
     5221 caatcggtct tagcttgccg gcgtacacgc aggctcgtgg gattaattac ataattgcat
     5281 acgctgtgcg cttccagcag gcggtgcagc gccccagttc ccggcgcgca cgcccctgca
     5341 ccgatcgatc agcgcgagca acccttgcca attgcaggcc ttcagcgtct gccggccgat
     5401 gctgtcggac agcggcgtgt tgaatgccac aacctagctc ctcttgctca attcgattgc
     5461 aaacaggcac cttgccgcct cctcatgcgt tgcggcgcgc gggttgctca ctgagcgggc
     5521 cgtcattcgg tctctcccac gcggctgggg ggtcggccat ctaaagccag caactgcttc
     5581 gcgttgcacg gcgatgccaa gcattaccgc gagtaccctc tggcaagcac gacggcgcag
     5641 gtcgtcacaa tgtcatctac gctcgcacca cgcgacaagt cgctgatcgg cttggaaaag
     5701 ccctgcaaga agggaccgac ggctttggcg ccgccaagcc actgggtcag tttgtatccg
     5761 atattcgccg agtccaaatc gggaaacacc agcacgtttg cccgcccagc cgttgcgcta
     5821 gggtctttca ctttcctttg cgcaaccgcg ggcacgagcg cggcgtccgc ttgcagctcg
     5881 ccatccaccg ccaggcgcgg cgcccgttcg cgcgcgagcg ccacggcgcg gcgcaccttg
     5941 tccaccctgg cgtggtgcgc gctcccgtgc gtcgagaacg aaagcatcgc gacacgagcg
     6001 ctctcgccaa gcagcctttc cgcgctacac gcggaagcca ccgcaatgtc agcaagctcc
     6061 tccgccgtcg gatcggcatt gaccgcacaa tccgcgaaga tcaaggtctt gggaccccgg
     6121 ccaagaaaat ccggcaccat catcaagaag aagctcgagg gcagcgcaat gccgtccgcc
     6181 agcccgaccg tcatcaagcc ggcttcaatg acccgccggg ttggattggc cgcgcccgcc
     6241 accatcgcat cggcgtctcc ttgatggacc atcatcccag caaagtacag cggctttgtc
     6301 accagacgct tcgccatgcc aggcgtcatc gtgcgccggc tggtcacgaa cgcggcagca
     6361 tacgactcca gccccgcagc gcgatggggg tcaatgattt ccaatccctt gacatcaagt
     6421 tccgcttgta cagcccgctg cgcgatcacc tcctcggaac cgaggagcac cgggactgcg
     6481 attttctcgt ttcgcaaacg gctggcagcc gcgagcacgc gcgcatcgtc gccttccggc
     6541 agcacgacct tgcgcaaatg ccgccgtgcg ctatcaaaaa gaccaccaag caagtccacg
     6601 tgtctcatcc cgtgagaaaa cgtcagcgac gggggccatc aatgaccgaa ataagcgagc
     6661 cgatcaagat tcaccgcggc ttgaaaggcg tctacttcga tcgctcaccc tgcacattca
     6721 tcgacggcaa ggcgggagag ctgcgctacc gcggttattc aattcacgat ctcgctgaaa
     6781 attccacctt cgaggaaacc gcgtggctgc tgctcaatgg cgagttgccc aacgcgcagc
     6841 agctctctgc cttcgacaag gacctcaaag cagcgcgcag cctgcctgag ccgatctatg
     6901 agatcatcca cgtcatgaag tctgcccatc cgatggacgt gttacgcacc gccgtctctg
     6961 cgctttcggc gttcgatccg gacgtcgccg acaattcgcg cgaggcgacg ctgcgcaagg
     7021 gcattcgcct cacctcacaa gtcgccatga tcgttgcggc gcattcacgc atccgcgagg
     7081 cgctcgaacc gataccggcc gaccgcgagc ttggccacgc ggccaacctg ctgtgcatgc
     7141 tgaaggggac aaagccgagc cgcgacgccg cacagctcat cgaccgcgac ctcattctgc
     7201 atgcggagca tggatcgaat gcctcatcgt tcacggcgcg cgtagtggtc ggcaccgaag
     7261 ccaatttgca cgcggccata acggcggcaa tcgctgcgct gtccggacct gcgcatggcg
     7321 gcgccgcgga agacgtgatg aaaatggcgg aagagatcgg tgatgctgcg cgcgccgccg
     7381 attatgtgcg ggcgaagcgc aaggcgggcg agccggtcac cggcttcggt catcgcgtct
     7441 atcgtgcgga ggatccgcgc gcgcgacata tgcgggccgg tgtagagaaa ctgtcgaagg
     7501 aaatgggcga gccacgctgg tacgaaatcc ttcaagcggt tgtgcaagcg atggcgccct
     7561 acgcgcgcca cggcgtcaac gtgaacgtcg atttctattc cggcgtcgtc taccacctgc
     7621 ttggcatcaa acgggatctg ttcgtgccga tcttcgccgt tggtcgcgtg ccgggctggg
     7681 tcgtgcaggt attggagcag atggggaaca acatcctgat ccgtccgctg acgctctatt
     7741 ccggcccgga gctgcgcagc tacgggtcga tcagcacgcg gtgactttgt gtggaatccc
     7801 actggctggg acgctgcgtc tcaatgcggc gttgcgggcg ctgtcctgtt ctgcttagct
     7861 ttttgagaaa ggcagtctca ctgaacgaga ataggtcgac cgggcaagtc cggcgatcaa
     7921 gggaggatcg aatgcacgtc gcgcgcaagc ttcttctgtg gagtgtcgtc gcttgcgtcg
     7981 gactaacgct cgcagcgatt ccggcacgcg cggaataccc tgaacgtccg atcgaactga
     8041 ttgtcccttg gggtcctggc ggcggcgcgg atcagcttgg gcgcctcgtc agcaaactgg
     8101 ccgaaccgct actcggccag ggcattcctg tcgtgaacgt gcccggcgcg accggcggga
     8161 ccggcatggc caagcttctg tcggcaccgg ccgacggtta ttccgcggcg atctatattg
     8221 ccgacagcca cgccctgctc gctggcaaga atccacgctg gagcatggac gacatcgcgc
     8281 ccgtcgcggt catgatccag ggcccctcct ttattttcgt gccgcaggac agccacttca
     8341 agacttggca ggacttcgaa aaggaagcga aggccaatcc aggcaggctg aagatcgcga
     8401 cactcggctt tgcgagcgtc gatgacttct ccatcagcgt gattgaacgc agcaaagggt
     8461 tgaaattcgt tcaggtgccc ttctccaaac cgggcgaacg ctatgtctcg atcctcggcg
     8521 ggcacgccga tgcactctac gagcaggcgg gcgatgtcgc atccttcctc aacagcaagc
     8581 agatgcggcc gttgctgatc ttcggcaagg agcgcttgcc tgcgttcaag gacacgccat
     8641 cctcgtatga actgggtatt ccggtcgcct tgccgcagtt ccgcgcgatt gtggtgcgct
     8701 ccggcacgcc gccggccatc gtgcataagc tatccgaaac gctggccaag gtcgcacaga
     8761 ctgacgaata caagaaattc ctggtggatc aattcggcga tgcaaacagc ttcaaggatg
     8821 catcgacggc tccggccttc gtgagtcagc agctggacga catgaaaaag gcaatggcgg
     8881 cgaactagtt ctcgatcgtt cggcgtttcg agcaagcagc catggacaag gcgctgttcc
     8941 gctcaatcgg tccctatgtg ctcggcttgg ccattgccgt tgccctctat atttacgcag
     9001 ggacgttcga atacacaccg cgggccggcg cgctcggtcc caatgtgtgg ccgtgcctgg
     9061 ccatcctact tatgggcgct tcgtgtctgt tcgagctgac gcgacgtctg atcatcggca
     9121 acaaggacgt ggtcggcttc atggaagcac tcgaccgaga gccggaagcg gaggaatccc
     9181 agcctgccta tccccgccgc gttatcggcg gtgtcgtgct catgggagtc tacgccatgc
     9241 tcgttccgat tctcggcttc gtcctgggaa cgtttctgtt cctcgccgcc ttcatgtata
     9301 tcggtggtta tcgcgcacat ggcgtcattt ggggcgccag cgctacggtg acgattttct
     9361 gcggcatcct gttcctgcgc attgcctatg tctcgctgcc gcgcggcata gaacccttcg
     9421 atcgcgtcac cgatggcttc tttgcaatcc ccagcatgtg gtgagcgtat gatcgacatc
     9481 atcacccaac tcgcggctgg gttcgtccac tgcttgacgc cgctcaacat agcaatgctt
     9541 ttgatcggca tcgtcatcgg cctgctgatc ggcgtgctgc cgggcttgac gctggtaatg
     9601 ggcgtcacgc tggcgctgcc gttcacctac aagatggaga tcaccgcctc gatcgtcctg
     9661 ctgacagcga tgtacgtctc cggcacctat ggcggcgctt tcaccgccat cctcttccgc
     9721 attccaggcg agccgatcga cgtgcccatg ctgtgggacg gctacaccat gggccgtaaa
     9781 ggcagaccgg cgaaggcgct aggctggacg ctggttgctg cgctgggcgg cggcttgctg
     9841 tcggcggtgg ttatggtcgc gctaacgcag ccactcgcta gcttcgcgct gcgcttttcc
     9901 tcgccggaat atttcgtgat cgtcatgttc ggcttgctga gcgtggtggc gatcggcaag
     9961 ggttcgctcg ccaacgcctt tatcagcatg agcgtcggca tcctgatttc gtcggtgggc
    10021 gttgacccgg tctatggcgc ctatcgtttc accttcggct tgccgatcct cgctgacggc
    10081 atcgaattcc tcgtggtcat ggttggcgcc tatggcgtcg gcgaggtact cacacgtctg
    10141 gataccggct ttgccaccaa gcccctcgac aagatttcca acgcgcgcac cgaattgccc
    10201 acgctgaagg aattaaacga gatcaagggc atgtttttgc gttcctcggt ggtcggcgat
    10261 ctcatcgggc tgctgcccgg tgccggcgcc accatcgctt ccttcgtaag ctacggcatc
    10321 gaggcgaggt tcggtaagcg gcgggatcag atgggcactg gcgtcgccga gggcatcgtg
    10381 gcgccgcagt cggcggcaac ggcctcggtc ggcggggcca tggttcacct gctggcactc
    10441 ggcattccgg gcagcggcgc aaccgcggtc atcctcggcg ccttcatgct acatggcatt
    10501 cagcccgggc cgcaggtgct cgtgaactcg gccagcatgg tctacacggt gtttgcctcg
    10561 ctgttccttg gcctcgcgct aatgtgcgca atcggctatt tcgccatccg cccgctggtg
    10621 aagatcctcg acttacccga ggcggtcgta tccgcctatg tgcttatcct ctgcttcatc
    10681 ggtgccttgt ccatccgcaa caatgtcacc gatttgtggt tgatcatcgg cttcggcgtc
    10741 ctcggctacg gcttcgagcg gttgaaattt ccgatcgccc cgctggtgct gggcgtgatc
    10801 ctcgggccca tcgccgagga agcctttatg aacacgatga tcagcttctc gaacgattgg
    10861 accgtctttt tcacgcggcc gatctccgga acgatcgtcg cgctaacgat catcgtgctt
    10921 ttgctgccgt tcgtacagca tctgatccga cgacgcttcg cgaccgcttg aggcttcaag
    10981 tgaggtgacc catgcgtagt agtcttaatc tcggtgcgag agcggtccag tcggccgggg
    11041 cgcgcggacg agtggcggaa ctgaggccgc tcacgccggc gggaaaatcg gcgactgccc
    11101 gcacgcataa gctcggcgcc acagcgcgct cccttgccat cctcgagcat gtcgctagct
    11161 cacaagcacc tgtggatgtc cttgacatca tcagctcgct aaaattgcca aaagcgaccg
    11221 cctaccggcg ggtcgaatgg ttcatcacac aaggctatct gtcacgagag ccgggccgcc
    11281 ggcggctgat cgttggcccc aagcttgcca atctggcctt cggcgcgttg tcgaccgcca
    11341 tacgcaacgg cgcctcgcac ctcgtgctcc agcgtctggt gcgcacgctc gacgaaacct
    11401 gcaacatcgg caccctcctc aacggcgagg tgatatatct cgatcgcgtg gaagcagagc
    11461 attggccgct ccggctacat tacaccaacg gctcgcgcgt acccctgcat tgcaccgcca
    11521 tcggcaagct gttcctggcg ctagcggcga cgccgcaccg acaacgcctg ctgcaaagcg
    11581 tggagctgcg ccgcttcacc gatagcacca tcaccgacaa agcacggctg gagaccgagc
    11641 tgcggcaaat ccgcaaggag cgagtctcgt tcgaccgcga ggaatatctt gtcggcgtag
    11701 tctgcatggc ggtcccggtg gtcggcaaga acggcgagat gctggccgcg ctcgcgattc
    11761 aggcgccgaa ggcgcgtatg gacgtgaata cggcgcgcgc ccatcttccc gcgctgcgct
    11821 gcgccgccag cgaacttggc gaaatctttc aggacagggg ctgaaactca ataagcattt
    11881 cccctgagca cgagaattaa acgtgatggc acccgccaaa gctagagagc taagaaggag
    11941 taggctatga agatgacgac ggaagaagcc ttcgtgaagg tcttgcagat gcacggcatc
    12001 gagcatgcct tcggcatcat cggttcggcg tttatgccga tctccgatct ttttcctcag
    12061 gctggcatta cgttttggga cgttgctcat gaagccaacg gcgctctgat ctgcgacgga
    12121 tttacgcggt cgaccggaaa aattgcgatg gtgatcgcgc aaaacggccc gggcatcacc
    12181 aacttcgtca ccgcgataaa aacggcctac tggaaccaca cgccgatgct gcttgtaacg
    12241 ccgcaagctg ccaacaggac cattggtcaa ggcggcttcc aagaagtgga gcaaatggcg
    12301 ctgttccgcg acatggtcgg gtatcaggaa gaggtccgtg atcctgcgcg cgtcgcggaa
    12361 gtcttaaatc gcgtcatatc gaaagcgaag cgtctgtcgg cacctgccca gatcaacatc
    12421 cctcgcgact actggaccca agtcatcgac atcagcctgc cggcgatcat tgaattcgag
    12481 cgtccggccg gaggcggcga cgccgtcgcc gaggcggcaa aattgctgtc agctgcaaaa
    12541 ttcccggtca ttctgtcggg cgcgggagtc gtacttggaa atgcaatccc cgagtgcgtc
    12601 gcgctcgccg agaagctcga tgcgcccgtt tgcaacaact atcagcacaa cgacagcttt
    12661 cctggcagcc accgattagc ggtcgggccg ctcggttaca acggttcgaa ggccgcgatg
    12721 gagctcgtcg caaaagccga cgtggtgttg gcccttggga ccaggctcaa tccgttttcg
    12781 actcttccgg gatatggcat cgattactgg ccgcggaacg ccaagatcat acaggtcgat
    12841 atcaatgcag accgaattgg tctgactaag cctgtcgctg ttggcatctg cggcgacgcc
    12901 aaacttgttg ctaagaacat tctggcgcag ctttcaccct cagccggaaa tgccggccgt
    12961 gaggagcgga aagcgttgat ccacacaacc aagtccaagt ggctccagac cctctccagc
    13021 atggatcacg aggaggacga tccggggacc acatggaatg cggacgtccg caagcgcgaa
    13081 cccgaccgaa tgtcgccgag gcaggcttgg cgcgcgatcc aggccggact tcccaaggac
    13141 gccatcatct ccagcgacat cggcaacaat tgtgccatcg gcaacgccta tccgaccttc
    13201 gatcaggcac gcaagtatct ggcgcctggt ctgtttgggc cttgcggtta tggcttcccc
    13261 gcgatcatcg gctccaaaat aggcaatccg aatacgcctt gcgtcggatt cgccggcgac
    13321 ggcgctttcg gaatttccat gaacgagatg agctcgatcg gacgggcgga gtggcccggc
    13381 atcaccatgg tcgtattccg caactaccag tggggcgctg aaaagcgcaa ttcgatcctg
    13441 tggttcgaca acaacttcgt cggaaccgag ctcaacccgc atctgagcta tgcaaaggtg
    13501 gccgaagcct gcggtctcaa gggcgtaaca gcaagaacgc aagccgaact gacggaggcg
    13561 ctgcgggagt catgtgaagc gcagaagaat cgagtaacga cctttatcga agttattctc
    13621 aatcaggagc ttggtgagcc gttccggcga gacgctatga agaaacccgt ggtcgtggcg
    13681 ggcatccggc gcgaggacac gcgtgcacag caaagcgctt agtggagctt gggcgtgtcc
    13741 caagaggccc gctcccgacg caatccgggg gcgggccgcg acatccgcga ggggcctgca
    13801 ggaaagttca ggagcagcat gacgatgcct gcgccaatga agacgccggt ggatcccagc
    13861 gccaatgcgg tggtcgacca actcgtgcag cgcggccgtg ccgctatggc cgagtttgcc
    13921 gatgccgacc agggccgtgt cgacgaggcg gtcacggcgc tcgcctggtc gctctacaac
    13981 cccgcacacg ccagagagct ggcggaacta gcggtcgccg acaccaagct cggcaatgtt
    14041 gccgacaaga tcatcaagaa gcaacgcaag accttcggta cgttgcgcga cctgctgcgg
    14101 gtaaaatcgg tcggcatcat cgaggacgac gccaagcgcg gtatcgtgaa atatgccaag
    14161 cccgttggcg tggtctgcgc cgtaacgcca tcgaccaatc ccggcgccac gccggtgaac
    14221 aaggcgatga tggcgatcaa gggccgcaac gccatcatca tcgccgcctc gcccgccggc
    14281 ctgaagacca ccatgcgcac agtcgattac atgcgggccg agctgaagaa gatcggcctg
    14341 cccgaggatc tggttcagat cctaccgcca ccggcgtcga aggacatgac ggaggcgctg
    14401 atgcgggcgg ccgacttggt ggtggccacc ggctcgctgg ataacgtgcg gcgcgcctat
    14461 tccagcggca cgccggcgat cggcgtcggc accggcaacg tgccggtgat catcgacgag
    14521 accgccgatc tcgacgacgc cgcgaagaag atctgcgcct ccaagatctt cgacaattca
    14581 acctcgtgct cctcggaaaa ctcggtgatc atcgtcgacg cggtctatga ccaagcgatg
    14641 gccgcgttag agcgcgccgg cggctaccgc gcctcggcga tcgagaagga gcggatccag
    14701 cgggcgctgt ggccggacgg aaaattgagt cgcattctga tcgcgcgcga cgccgacgtg
    14761 ttcgcccgcg gctgcggatt gccggcggca accgaaggca agaagttctt catggtggaa
    14821 gagaccggcg tcgggcccga ttcgccattc tccggcgaga agctgtcgct ggtcctgacc
    14881 gtctaccgcg ccaaggattt cgacgctgcg accgagcaag ccaatgccat tctcactttc
    14941 caaggtcgcg ggcattcggt cggcttgcac accaaagacc cgaagcgcgg ccgcgccctg
    15001 gcggaacgtt tggacgtggt gcgcgtgctg atcaaccagg cgcacacctt cggcaacggc
    15061 ggcagcttcg acaatggcct caatttcacg ctgagcatgg gctgcggcac ctggggcggc
    15121 aacagcatca ccgacaacct caactacaag aacttcctca acatcaccca cctcgtgact
    15181 gcgattcccg aggacaaacc gtcggaacaa gaactgttcg gcgcctactg ggccaaatac
    15241 ggccgctgag gtaccgaatg aattttgaag aatacgtcgc gaaatcgctg attctcgcgc
    15301 ccgccggaat agcggtcccg cgcgggcagc tttgcaccac gccggacgaa gtcgccgtgg
    15361 cagtgcgcag catcggcccc gccgtgatca aggcacaagt cgctaccggt aagcgcggca
    15421 aagccggcgg catcagattt gtaagcacgc cgccagaggc ggagcgcgtc gcttcggcgg
    15481 tcctgggcat gacgatcggc gagtaccggg ttgaacgcct gctggtcgag gaacaggcaa
    15541 aagtagcgcg tgaattctat gccgcggtgc taaatgacgc ccaagagcgc aagccgctca
    15601 ttctatttgc gaccgaaggg ggaatggata tcgaggagat cgcggcggcc aaccctgacg
    15661 cgatccgccg gctgccgatc gacattgatg ccacgccatc tgccgccgac attgcgcgaa
    15721 tcctggaagg atcggggctg ggaaatgccg aagcgagtgt tgccgacatt ctttcccgcc
    15781 tctatgcggc ctaccgccaa cgggacgccg aactgctgga aatcaacccg ttggcgcagc
    15841 ttgccgatgg acgcgtagtc gcactcgatg ccaagttcgt gctcgacgat tcggcggcct
    15901 atcggcaaga agagattgca aagcttgccg cgcttgcgcc gatgaccgcg cttgaacggc
    15961 tcgccgccgt gcacggacta aagttcattc agctcgacgg gaatgtcggc gtgttggcca
    16021 acggcgcagg cctcaccatg acgacgatgg atgtcattcg ccattacggc ggaaggcctg
    16081 ccaacttcct agagatcggc ggcgaggcct ataccaagtc cgagatcgcg ctcgacctcg
    16141 tgctgtccaa ccccggcatc aaaagcctgg tgatcaattt ctgcggcgcc ttcgcgcgca
    16201 cggacgtgat ggccgatggc gtcgtcaaag cctggaaaga gctcaagccg agcattcccg
    16261 tgtttttctc aatccacgga acgggtgaag aagaggcgat tgcattggta cgcgaaagcc
    16321 tccgcatcga accttacaag tcgatggagg acgccattca ggcggcggta cgtgtagcgc
    16381 aatgatcgta cgcaagtccg ataaagtgat cgtgctcggc attaccggcc gccagggcac
    16441 cttctggacc gacaaaatgc tcgcctacgg cacgcaggtg gtcgccggcg tgaatcccaa
    16501 acgcgccggc gagtaccatt gtaacgtgcc aattttcgcc tcgaccgcag acgcggtgcg
    16561 cgaaacggat gggagcgttg ccgttatgtt cattccagct tccgtggcgc gccatgccgc
    16621 aatcgcggcc atcgaatcca gaatcaggct cttggtggtg cttaccgaac acattccggc
    16681 ccaggatgtc atggccgttc ttgccgctgc ggcgcggcac ggcacgcgcg tagtcggacc
    16741 gaacactgcc ggcttggtga cgccagggga atgtttcgtc ggcatcatgc ccgccttcgt
    16801 gccgagtgta ttcaagccag gtcgcgtcgg cgtcatttcg cgcagtggca gccttggcac
    16861 actcatctgc ctcaagctga cgcaagccgg agtcggtcaa tccgcctttg ttggaatcgg
    16921 cggcgatccg atgctgggca caactagccg agatgcattg gaagcgctcg atcgcgaccc
    16981 cgcaaccgat gctgtggtca ttgtcggcga aatcggcggt acgatggaag aagaagccgc
    17041 cgaatacgcc aagacgatga acaaaccgat cgcggccttc atcgctggcg ccgcgtcacc
    17101 gcccggcaag aagatgggtc acgcgggcgc catcgtgttg ggaaatcgcg gcagctatgc
    17161 ctcgaaacgc aaggcgctcg aagctgtcgg cgtaatcgtc gtcgataccc cggccgaaat
    17221 tgtgccagcg ctcgccatca gagacctcgg caaatgattg gccacatacg gctccgttaa
    17281 aattccattt acctacgagt cgatcatttc ggcgcggtcc atgacgagtg agctcgacgc
    17341 acccatgcac gtgatcgaac agctcacaaa gccttgcttg atgatagtga gtcgtcctcg
    17401 ttgctggagg ttgacccagg cagccgtggt atgacgggtc ggtcttgaaa cgcatggcca
    17461 tctccaattc gagcggcacg tcgcccgccg tgcaacgtta ggggctccgc tttcgccgag
    17521 gcaggatgga atcgcatatc cctggcgcca tgggttgtgc ttcgacgatc cgtgtttcgc
    17581 tggcaaagcc ccaaatcgga tcaaaccggt ggacctgata gaccggacgt accttcatgt
    17641 gaggcggata ctcgcctttg cgcagcgtcg tcgcgataca ctgaaccact gtcgccggga
    17701 tgcgtccgac atgacccgca gcagcgcgat gaacatggcc gctgaaaacg gcgacgaccc
    17761 gcccagagtg ctgcaaagcc cgacccagcc gcgacgccgt ctccgttgtt tcgaagtgga
    17821 gccgatccgg ccccaccgaa acctcaaatg gcggatggtg cgtgaaaacc gcgataggct
    17881 tcgtggtctc ggcgtcgatc agctcaatca aacgtctgac ccgctcaagg caaaagtcac
    17941 ccttgttgct gcgtgaactc agggtatcca gcgcaatcag tctcaccggg taatcgtcaa
    18001 tcgcatagtc gatgaagtcg gaatcgggcg caagatatcc ggatgccgaa aaggccgcgc
    18061 gtagattcgc cctgttatcc ctattgccgc ccaggacata taccggcgca cgcgctttcg
    18121 cgagcgtggc tactgcctga gcgtattcgt cttgccgtcc gttatgaacg atatccccgg
    18181 tgtgaacgat gacgtcgggg gccggatcga gcgcgtttat gtccgcaatc gtgagtgcga
    18241 aatcgcgtat tctttggtcg gcatcgggtg tatccagggt taaatgggta tcggagatct
    18301 gcgcgatgat catgatgccg gactctggcc tgtcgatcgc aggcctgcgt agtactcctt
    18361 gaggatcgcc accacctccg gccgactaaa ctcgggcggt atgtccgcgt tggaggagag
    18421 catttcacgc tgctcggtac cggagatcga aatgtgatgc tgcggatcgt ggggacaggt
    18481 ctttgccgtc gccattccgt agcacttccg gcagtaaaaa gtgatgtcga tcttcagcgg
    18541 cttggtatga agtgcgccgg gccacagctg atcgaagatg tgatgcgcat cgaaggggcc
    18601 gtagtagtcg ccgacgccgg catggtcgcg cccgacgata aggtgcgaac agccgaagtt
    18661 ctggcgaatg agggcatgaa gcagcgcctc gcgtggaccg gcatagcgca tctcgatcgg
    18721 atagcccgcc tggatgaccg ttcccgcgcg gaaataattg tcgatcatcg cctgaattgc
    18781 tcgggtacga acatcggctg gaatgtcgcc tgctttcaat ttcccgagca cctgatgaat
    18841 gaaaacaccg tctgatacct caaccgcgat ttttaccagg tgctcatggc tacggtgcat
    18901 gggatttcgg gtttggaagg cggcgacatt ggaccacccc cgctcaagaa acatggcccg
    18961 tgatcgggcg gggcggatat agaggtccgg gtatttggca ggatattcgc cttcgctaag
    19021 caccatgatc ggaccggcca ggttaacgtc gccctgatgc agtaccttcg cgacgcctgg
    19081 atgcttggga tcggcggtgc gatagacgtg ttcggcctcg agttgtttgt cgattgagta
    19141 tttctcgtga atcttcatca cggcgagtat ttcactggtc tccgcgtcga tgagcgtcac
    19201 ctcctgttcg gcatgcaggc tatcggcaag gtccttgttt accgaaagcg tgatcgggat
    19261 cggccagaac acgccgctgg tcagtttcat gtccaggcac gaaccgcgcc agtcgtcatg
    19321 ccccatgaaa ccatcgagcg gcgtataggc ccccattgcg agcatgaaga cgtcggagat
    19381 ggtgcgcgag tcgagaggaa ttttcttgaa tttctccgcc cgcttgcgtt cctcggcccg
    19441 ctccgtctcc agcagcaaca gtggcttgac ctgctccgaa ttgtgcggcg gcacgagttg
    19501 ggacactgcg atccttcgta aatcagaatg tctcggaaag gatgaccttt gcagactgga
    19561 ctggctggcg tggctcggcg ctcatggaag aattgccccg acacacgcaa gacacgggag
    19621 acacagcgtt ctgagcaggc cggtgatcaa tctacgatgt gatacaccgg agccttctgc
    19681 atctcccact tcccggagtc ccggtcgtag cgagacaggg tgaagcaatg ccaatctttg
    19741 tcgtccagct tcggatgatc cgcccggtaa tagtatccag gccaccgcgt ttccgtccgg
    19801 aacagggtgt gctgcgtgac gcattccgag gccaagactc ggtgttgaag ctcccatgcg
    19861 cgctgaagct ggtgaaggtc ttcggccccg agatgggcga gatcttcctt cagcatgccc
    19921 agcaactcaa gcccgcgggc gagtagcggt tcgttggtcg tatagtgggc gccgatgccg
    19981 ccgacatatt cgtccatgat cttttcgagc cgctgaaggc catgaatcgg caaaatatag
    20041 ctcggcgaga cggttcctgc gacgatttcg ttgcgcccga cctggtagtt ctctagcggc
    20101 ttgtagatgg ccttcctgag gtcttcgtac tccgtctcac tgacctgagg cggctgtttg
    20161 ccgaactcat ttacgtacct gaccgctgcc ttgccggcga tgcggccctc cgtgaaggag
    20221 cccgacgaga acttgtgggc gctgccgccg atcgcgtcgc cggcgccgaa gaggccttca
    20281 acggtcatca tccggttgta tccccactgg tattccgagg gcgcgtagtc ttccggaccg
    20341 ctcgcccacg cgcccgaaca cgtcgcatgg gagcccatga cgtagggctc cgacgtggtc
    20401 agttcggggt ttatt
//
LOCUS       DATLJT010000833         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4189510,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000833 DATLJT010000000
VERSION     DATLJT010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189510"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VM910_18930"
     CDS             complement(<1..588)
                     /locus_tag="VM910_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HVI64635.1"
                     /translation="MYVAVKGGERAIENAHRLMAHERRGDRDVPEVSLAQISEQLALA
                     VDRVMSEGSLYDRELAALAVKQARGDLIEAIFLVRAFRATLPRFGASEPVDTGRMRVA
                     RRISSTFKDIPGGQILGPTFDYTHRLLDPQLGEGFLPEQPARSEAADAATPRVTDILG
                     RDGLIEPSPQQDADTPVGDLTREPLNFPAERDLRLQ"
     gene            complement(592..1200)
                     /gene="phnH"
                     /locus_tag="VM910_18935"
     CDS             complement(592..1200)
                     /gene="phnH"
                     /locus_tag="VM910_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HVI64636.1"
                     /translation="MTTIAELPAGFADKVLSAQSTFRSVMDAMAHPGSVQQAAVSVGA
                     PRPMMRGTAAIALTLFDHDTPIWLDAGMSETSDVARWLKFHSGAPVVGDPSICHFALI
                     ADANALPDLARFALGTNEYPDRSTTLVIQVESLTQGRTYELRGPGIDGSATLRATLEP
                     NNLFERLAINETLFPRGIDLLLVSGEEIVAIPRTTRLIGHGG"
     gene            complement(1204..1680)
                     /gene="phnG"
                     /locus_tag="VM910_18940"
     CDS             complement(1204..1680)
                     /gene="phnG"
                     /locus_tag="VM910_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HVI64637.1"
                     /translation="MSARGEGDRNDDGRQALRKAAMAVLTHASAAEIAGYLGAITVPA
                     HENLRDAESGLVMVRGRIGGDGAPFNLGEATVSRAAVRLASGEVGFGYTLGRDREKAR
                     MIALCDAMAQSDEFAASVEARVIAPLRAAISARRDRKAAEAAATRVDFYTMVRGEG"
     gene            1812..>2198
                     /gene="phnF"
                     /locus_tag="VM910_18945"
     CDS             1812..>2198
                     /gene="phnF"
                     /locus_tag="VM910_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HVI64638.1"
                     /translation="MSMQDSAYGVALWRQVADGIERGIADGTFAAGERLPGETEIAET
                     YRVNRHTVRRALATLAERGLVRAERGSGTYVEAHRLAYPLRARTRFSEIVGAGGQEPR
                     GQLIDASEDVATRDLARMLGLKTGAPL"
BASE COUNT          395 a          738 c          681 g          384 t
ORIGIN      
        1 ctgcagacgc aaatcgcgct cggccggaaa attcagcggc tcgcgcgtga ggtcgccgac
       61 cggcgtatcc gcatcctgct gcggcgacgg ttcgatcagg ccgtcacggc caagaatatc
      121 cgtgacgcgc ggagtggccg catctgcggc ttccgatctt gccggctgtt cgggcagaaa
      181 gccttcgccg agttgcgggt ccaacaggcg atgggtgtag tcgaaggtgg ggccgaggat
      241 ctggccgccg ggaatgtcct tgaacgtcga ggagatccgc cgcgccaccc gcatcctacc
      301 ggtatcgacg ggctcgctcg cgccaaagcg cggcaaggtt gcgcggaaag cacggaccag
      361 gaagatcgcc tcgatcaagt cgccgcgcgc ctgcttgacg gcgagtgccg ccagctcgcg
      421 gtcatagagc gagccttcgc tcatcacccg atcgaccgca agcgcaagtt gctcggaaat
      481 ctgggcaagc gaaacctcgg gcacgtcgcg atcgccacgc cgctcatgcg ccatcagccg
      541 atgggcgttt tcgatggcac gctcgcctcc tttgacggcg acatacatgt ttcaacctcc
      601 gtgaccgatc agccgcgtgg tgcgcgggat cgcgacgatc tcttcgcccg agaccagcag
      661 cagatcgatg ccgcgcggga acagcgtctc attgatggca agccgttcga acagattgtt
      721 aggctcgagc gtggcccgca acgttgcgga gccgtcgatg ccgggtccgc gcaactcgta
      781 tgtacggccc tgcgtcagac tctcgacctg tatgaccagc gtcgtcgagc ggtccggata
      841 ttcattggtg ccgagcgcga aacgcgcaag atcaggcagt gcatttgcat ccgcgatcag
      901 cgcgaaatgg cagatcgagg gatcgccgac aacaggtgcg ccgctgtgga atttgagcca
      961 tctcgcgacg tcggacgtct ccgacatacc ggcgtcgagc cagatcggcg tgtcgtggtc
     1021 gaacagagtc agcgcgatcg cagccgtccc acgcatcatg ggtcgcggcg cgccaacgga
     1081 aaccgcggcc tgctgaacgc tgcccggatg cgccatggca tccatcaccg agcggaatgt
     1141 cgattgcgcc gacagtacct tgtccgcaaa tccggcgggc aactcagcga tcgtcgtcat
     1201 ccctcaaccc tcaccgcgca ccatcgtata gaagtctacc cgcgttgccg ccgcctcggc
     1261 cgctttccgg tcgcgtcgcg ccgatatcgc cgcacgcaac ggcgcgatca ccctcgcctc
     1321 gacgctggct gcaaactcgt ccgactgtgc catggcatca cacagcgcga tcatccgcgc
     1381 cttctcgcga tcacggccaa gcgtgtagcc gaagccgacc tcaccgctcg ccagccgcac
     1441 cgcggcgcgc gaaacggtgg cctcgccaag gttgaagggc gccccgtcgc ctccgatccg
     1501 gccccgcacc atcacgaggc cactctcagc gtcccggagg ttttcatgcg ccggcaccgt
     1561 gatcgcgccg aggtatcccg caatctcggc cgcgctcgca tgcgtcaaca cagccatcgc
     1621 cgctttgcgc agggcctgcc ggccgtcatc atttctgtcg ccttcgccgc gtgcgctcat
     1681 gcttggcctt gcgctcttgc ctgcctgaat caagttgtct atgataatag acaacttgat
     1741 aatcaaacgc catgaagatt tcgtgacaaa cgcatgattt tccggacttc ctaccaagga
     1801 cttgctgcgt catgagcatg caggactccg cctacggcgt cgcattgtgg cggcaggttg
     1861 ccgacgggat cgagcgcggg attgccgatg ggacgtttgc cgccggcgaa aggctgccgg
     1921 gcgaaaccga aatcgccgaa acctatcgcg tcaatcgcca caccgtgcga cgggcgctgg
     1981 cgaccctcgc cgagcgcggc ctggtgcgcg ccgagcgcgg cagcggtacc tatgtggagg
     2041 cgcatcgcct tgcctatccc ttgcgcgcac ggacgcgatt ttcagaaatc gtcggtgccg
     2101 gcggtcagga accccggggg cagttgatcg acgcgtccga agatgtcgca acgcgcgatc
     2161 tggcgcgtat gctcggtctg aagaccggcg cgccgctg
//
LOCUS       DATLJT010000834         3536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1892122,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000834 DATLJT010000000
VERSION     DATLJT010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892122"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..648)
                     /gene="rplL"
                     /locus_tag="VM910_18950"
     CDS             complement(271..648)
                     /gene="rplL"
                     /locus_tag="VM910_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HVI64639.1"
                     /translation="MADLQRLVDDLSKLTVLEAADLAKMLEEKWGVSAAAAVAVAAAP
                     GGAAAAAPAEEKTEFTAVLTAIGEKKIEVIKEVRALTGLGLKEAKDLVEGAPKPVKEG
                     VSKDEAEKIKAQLEKAGAKVELK"
     gene            complement(693..1214)
                     /gene="rplJ"
                     /locus_tag="VM910_18955"
     CDS             complement(693..1214)
                     /gene="rplJ"
                     /locus_tag="VM910_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HVI64640.1"
                     /translation="MDRAAKKELVTALNGVFKATGVVVVAHYSGLNVAQMQTLRRQMK
                     QAGAAVKVAKNRLAKIALEGTDAAVIVPLLKGPTLIAYSGDPVAAPKAAADFAKAHEK
                     FVILGGAMGKTALDANGVKALAALPSLDELRAKIVGLIQAPATKIAQVVNAPAAKVAR
                     VVQAYAAKSEAAA"
     gene            1818..2333
                     /locus_tag="VM910_18960"
     CDS             1818..2333
                     /locus_tag="VM910_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64641.1"
                     /translation="MAIAAGVAFALVHFSFAAIAQGLGHSEVKQIQLTEKQLQGVIAA
                     QKDVAAVLDKLQGAPPPDQLPPQVLSELDAAARKHGFKDFNDYDEVVGNITMVMAGID
                     PKTKAFTEPADAIKKEIADVTADKTIPAQEKKQMLEELNEALKAAQPIQFPGNIELVK
                     KYYDKIDAALN"
     gene            2610..>3536
                     /locus_tag="VM910_18965"
     CDS             2610..>3536
                     /locus_tag="VM910_18965"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64642.1"
                     /translation="MTANRILTTHVGSLVRPPQLVEFLHKIEDREPYDKAAYEACLKQ
                     SIDTVVRRQAEAGIDIVSDGEFSKGRNWAFYVHDRLSGVASRPLTTEEAKDPMAAVGG
                     GQDRVAFPEFYAEYDRLSGLSKRLGSRFVVNGALSYSDAQVKRDIANLKAAAAKVKVA
                     GAFLPVVAPASALPNAKNEHYPDEQSLLCALADCLHQEYKAIVDAGLYVQIDDAFLPY
                     MHEKMVPPMTHAQYRAWAQLRIDALNRALKGIPQERSRYHICWGSWNGPHAFDVPMKD
                     IVDLMLQVNVGAYQFEAANPRHEHEWVVWRSVK"
BASE COUNT          662 a         1174 c         1079 g          621 t
ORIGIN      
        1 gacttcctgg attttcccga aaaacttgcg aacgcgcttg cgaccggtaa acgtctgcgc
       61 catctcatct ctcgctttgt cgaaccgtcg ggcccgggac gactctccgg aaggccgcca
      121 tccatcgcga ccatcaggac agccgtcgag acccctctcc tcatgcgatt cggccgaccg
      181 ccgaaccgcc atgtccctgc cgccgtccgg cagagatatg ggcgattccg gggtttactc
      241 accccggaat cagttgcgtt gacttcacac ttacttgagt tccaccttgg ccccggcctt
      301 ttccagctgg gccttgattt tctccgcctc gtccttgctg acaccttcct tgacgggctt
      361 gggcgcgccc tccaccagat ccttggcctc cttgaggccc aaaccggtga gcgcgcgcac
      421 ctccttgatc acctcgatct tcttctcgcc gatggcggtg agcacggcgg tgaattcggt
      481 cttctcctcg gctggagcgg cggcggcggc gccaccggga gcagccgcca cggccaccgc
      541 ggccgcggcg gaaacgcccc acttctcctc cagcattttc gcgaggtcgg cggcctccag
      601 caccgtcagt ttggaaaggt cgtcgacgag tctctgcaga tcagccattg gacatcctta
      661 tgagttcgaa ccagtcgagt ttgcgatgcg cgtcacgccg ccgcctcgct cttggcggca
      721 taggcctgga ccacgcgggc gaccttggcc gcgggcgcat tgacgacctg cgcgatcttg
      781 gtcgccggcg cctgaatgag gccgacgatc ttggcgcgca gctcatcgag cgaaggaagc
      841 gccgcgagcg ccttgacgcc gttggcatcg agcgcggtct tgcccatcgc tccgcccagg
      901 atcacgaatt tctcgtgggc cttggcgaag tcggcggcgg ccttgggcgc agcgaccgga
      961 tcgccggaat aggcgatcag ggtcggcccc ttgagcaggg ggacgatcac ggctgcgtcc
     1021 gtgccttcga gcgcgatctt ggcgagacgg ttcttggcga ccttcaccgc cgcgcccgct
     1081 tgcttcatct gcctgcgcag cgtctgcatc tgggcgacgt tgaggccgga gtagtgagcg
     1141 acgaccacga cgccggtggc tttgaagaca ccgttgagcg ccgtcacgag ctccttctta
     1201 gccgctcgat ccactgcaag ctctttccgg ttggcggccc gcgttcacat gcgggaaccg
     1261 ccggttgcga ccgccatcct gaccttgcgc ttaacaaacg ccggcaggac ggcactgctg
     1321 agatcctgcc ccccaggcgc ggacggaagc cgcaccgggc tctcgaggtt cgaaccggtc
     1381 gcggggcgcg cggccccaaa acggtcttcc ccgtctgtgc aggcaattcg cattaagccg
     1441 ggagccggca cctgcagtct tggacaggac ggacttccgg aaaccgccgg aaggccttgg
     1501 cgcccaaaga aatctcgcgc ttggccccgc ctcctttcag ataaatcccg caggacgtcc
     1561 aaaaggaata aggtcctaac cgccatgttt tccttgaagc gcgcaaaggc gcgccgacaa
     1621 cactgggccg gcacgccctg gcctcctatg tagcggtttt caccggtttt gcaagcataa
     1681 tcacgccgat tgcttggatt tttgggggcc gccgcaggtc caattttggt catcggcgag
     1741 cgctaggaaa ccggcggtcg cgactcacac ttccagccag cagagagggg gctaaaggga
     1801 tgcaacagag acttgctatg gccatcgccg ccggggtggc ttttgccctc gttcatttct
     1861 cctttgctgc gatcgcgcag ggcctggggc actccgaggt gaagcaaatc cagctcaccg
     1921 agaagcagct ccagggcgtt attgccgcgc agaaggacgt ggcggcggtc ttggacaagc
     1981 tgcagggggc gccgccaccg gatcagttgc ccccgcaagt cctctccgag ctcgacgcag
     2041 ccgccaggaa gcacggcttt aaggatttca acgattatga cgaggtggtg ggcaacatca
     2101 ccatggtgat ggccggcatc gatcccaaga ccaaggcatt caccgagccc gcggacgcga
     2161 tcaagaaaga gatcgcggac gtcaccgccg acaaaaccat tccggcacag gaaaagaagc
     2221 agatgctgga agagctcaac gaggcactga aggcggcgca gcccattcaa tttccgggca
     2281 acatcgagct cgtgaaaaag tattacgaca agatcgacgc cgcgctcaat tagcgcccgt
     2341 cccggttgcg tgaactccat gcgatgtccg ttcgtccccg cgaggggcac cccgcgacag
     2401 caaaagcgcg ttcacgcgcg tctgtgacgc gctatgcgtc gcggggatcc cgttgcgggg
     2461 acgaacggcc caatccgcgc cgatcaacac gaacgcggcg tttgctcacg ccgatttttg
     2521 ctcggcctca ccgcccgatc gttgctgccc gggcagtgct caggtgataa aaagagggga
     2581 gcgcgcaagc caagaacgcg gggaggaaaa tgaccgccaa ccgcatcctc acaacccatg
     2641 tcggcagcct ggtccggccc ccgcagctgg tcgagttcct gcacaagatc gaggatcgcg
     2701 agccctacga taaagccgcc tacgaggctt gtctgaagca atcgatcgac acggtcgtgc
     2761 gccggcaggc cgaggcgggc atcgacatcg tcagcgacgg cgagttcagc aagggccgca
     2821 actgggcctt ctacgtccac gaccgcctca gcggcgtcgc cagtaggccg ctgaccaccg
     2881 aagaggccaa ggatcccatg gccgccgtcg gcggcgggca ggaccgcgtc gccttccccg
     2941 aattctatgc ggaatacgac cgcctttcgg gcctgagcaa acggctcggc tcgcgcttcg
     3001 tggtcaacgg cgcgctgagc tattcggatg cgcaggtcaa gcgcgacatc gccaatctca
     3061 aggccgcggc agccaaagtg aaggtcgcag gcgcgttcct gccggtggtc gcgcccgcaa
     3121 gcgcgctgcc gaacgccaag aacgagcact atcccgacga gcaaagcctc ctctgcgcgc
     3181 tcgccgactg cctacaccag gaatacaagg ccatcgtcga tgccgggctc tacgtgcaga
     3241 tcgacgacgc tttcctgccc tacatgcacg aaaaaatggt gccgccgatg acgcatgccc
     3301 agtatcgcgc atgggcgcag ctgcgcatcg atgcgctcaa ccgcgcgctc aagggcattc
     3361 cgcaggagcg ctcgcgctat cacatctgct ggggcagctg gaacgggccg catgccttcg
     3421 acgtgccgat gaaagacatc gtcgacctga tgctgcaggt gaacgtcggc gcctatcagt
     3481 tcgaggccgc caatccgcgc cacgagcacg aatgggtggt ctggcgatcc gtcaag
//
LOCUS       DATLJT010000835         1806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3959445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000835 DATLJT010000000
VERSION     DATLJT010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VM910_18970"
     CDS             complement(<1..630)
                     /locus_tag="VM910_18970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64643.1"
                     /translation="MMDFLHAKWWLIALHAVLAIAAPAHAQTFPSKTIRIIVPYPPGG
                     SIDLTGRVIAKNLQDSVGQTVIVENKPGANAAIGIEALLRSDPDGHTLAILSDSPVTI
                     NVHLARLNYDPLTDLVPISRAVSSPIILAANAKAGIASIADLIAAAKAKPLSYAVAAR
                     GSVSHLAAELLQRELGITMQAVPYRGGAPAAVAIASGEVPLGLFDTAAIL"
     gene            944..>1806
                     /locus_tag="VM910_18975"
     CDS             944..>1806
                     /locus_tag="VM910_18975"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI64644.1"
                     /translation="MSSNCRIAIVGGGLAGLAAAHALRTFGMQVEVFEAAPALGEIGA
                     AVNVSPQAFKALQAIGLGDKVAAVATSSPGIYTRNMQTGEFLEFNDRHKAAARYGAPY
                     YTFHRADLLDAFASGLDHSAIHLGHRLTGLEERGDRTILAFANGARVEAEMVIGADGV
                     RSVIRRALYGDDNPAYTGQMVWRALLNGDDVPEEVLEPTGHVQWVGPGCHLLVYYIRG
                     KKLVNIVTQEDTDKWVEEGWSIRGDPDEMRLSFPNPEPHLEKLLSLVTECSKWGLFTR
                     PLTHNWGRGRI"
BASE COUNT          328 a          546 c          605 g          327 t
ORIGIN      
        1 caggatcgca gccgtgtcga acaatcccaa ggggacctcg ccggaggcaa tcgcgaccgc
       61 cgccggtgct cctccgcgat agggcacggc ttgcatcgtg atcccgagtt cacgctggag
      121 caattcagcc gcaagatgcg acaccgaacc gcgagctgca acagcatagc tcaagggctt
      181 ggccttcgcg gccgcgataa gatcggcaat cgatgcgatg cccgccttgg cattggcggc
      241 caggatgatt ggactgctca ccgccctgga aatcggcacc aggtcggtga gcggatcgta
      301 gttgagccgc gccagatgga cgttgatcgt gaccgggctg tccgagagga tggcaagcgt
      361 atggccgtcc ggatcgcttc gcagcagggc ttcgatgccg atggcggcat tcgcgcccgg
      421 tttgttttcc acgatgacgg tctgtccgac gctgtcctgc agattctttg cgatcacgcg
      481 cccggtcagg tcgatgctgc cgccgggcgg atagggcacg ataattcgga ttgtcttgct
      541 cgggaatgtc tgcgcgtgcg ccggtgcggc gatggcgagg accgcgtgca aagcgatcag
      601 ccaccacttc gcgtgaagga agtccatcat gcaagattcg cattcgacgt ccggctgtcc
      661 gcgctgatga tggacgcaac acggtgggag gagcaagccc aacacgagca tgctccgggt
      721 ccccgcaacg gggtcccgcg acgcatagcg cgtcgttaag ggcgttcacg cccgtcttcg
      781 acgtgctatg gacgcgcgta aacgcgcttt tgctgtcgcg gggtgcccct cgcgggggac
      841 gaacggatga agatgggcaa accgagcgac gcgacaagcg gggttctgct atgctcgaac
      901 ggtcgcggct gggatcggtg ccggcgcggg tctggaggga cgagtgtcgt cgaactgtcg
      961 gatagcgatt gtcggcggcg ggctcgccgg gctcgcggcc gcgcatgcgc tgaggacctt
     1021 cgggatgcag gtggaggtct tcgaggccgc tcccgccttg ggcgagatcg gcgccgcggt
     1081 caacgtaagt ccgcaggcct ttaaagcgct gcaagcgatc ggtcttggcg acaaagtcgc
     1141 cgctgtcgcg acctcatcgc ccggaatcta cacccgcaac atgcaaacgg gtgagttcct
     1201 cgaattcaac gatcggcaca aggctgcagc gcggtacggg gctccctatt acacctttca
     1261 ccgcgccgac ctcttggacg cgttcgcaag cgggctcgat catagcgcca ttcatctggg
     1321 acatcgcctg acaggattag aggagcgagg cgatcgtact atcctcgctt tcgccaacgg
     1381 cgctcgagtc gaggcggaga tggtgatcgg cgccgatggc gtgcgctcgg tcatccgccg
     1441 ggcgctctat ggcgacgaca accccgccta taccggtcag atggtatggc gtgcgctcct
     1501 caacggcgac gatgtgccgg aggaggtgct cgagccgacg ggccatgtcc agtgggtcgg
     1561 tccgggttgc catctcctcg tctattacat ccgcggcaag aagctcgtga acatcgtcac
     1621 ccaagaggac acggacaaat gggtggagga aggctggtcg atccgcggcg atcccgacga
     1681 gatgcggttg agctttccca accctgagcc gcacctcgag aagctcttga gcctcgtgac
     1741 cgagtgctca aaatggggcc tcttcacgcg gccgctcacg cacaattggg gccgcggtcg
     1801 cattga
//
LOCUS       DATLJT010000836        10314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3500562,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000836 DATLJT010000000
VERSION     DATLJT010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500562"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /gene="rpiA"
                     /locus_tag="VM910_18980"
     CDS             <1..646
                     /gene="rpiA"
                     /locus_tag="VM910_18980"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HVI64645.1"
                     /translation="RDGMKLGLGTGSTAKHFVALLGERVRAGLKVVGVPTSEATRADA
                     ERCGIALTSLDEIDRLDLTVDGADEIDPQLNLIKGGGGALLREKIVAAASDRMIVIAD
                     ESKWVAALGRFPLPIEVIPFGLAATQRAVVQAFAECAVSGQMAVRKARDGHVFVTDGG
                     HWIVDAHLGRIEDAPRLAGLLGAIPGVVEHGLFIGLASTAILAGAQGIRVVERQ"
     gene            674..1198
                     /locus_tag="VM910_18985"
     CDS             674..1198
                     /locus_tag="VM910_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HVI64646.1"
                     /translation="MKSVFRILPAAGLALALAMAALPAAAQQAAAPPKAASPGAIAAA
                     KEILAMKNASAMYQNAVPNIVQQTKDTLLQTNLNYQKDLNEVAVIVAKNLAGREKEIG
                     EGMAQIYASEFTEQELKDLVTFYKSPLGQKLLANEPRAIQSSMAYMNQWAQQFGEIVN
                     GEFRAEMRKRGKQI"
     gene            1204..2589
                     /gene="gor"
                     /locus_tag="VM910_18990"
     CDS             1204..2589
                     /gene="gor"
                     /locus_tag="VM910_18990"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HVI64647.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGHGARVMVAEEYRMGGTCV
                     IRGCVPKKLFVLGSHVRQEIEDAAGFGWSIPSATFDWPTLVANKDKEIARLEAAYTAN
                     VEKSGARIVKTRAVFEDPHHLLLATGETVRARYVLVATGGAPNHGPIIPGVEYVISSN
                     EAFHLRELPKRIVIQGGGYIALEFACIFAGYGSDVTVIYRGENILRGFDDDVRTHVRA
                     ELEREGITILTGNTITKVDKHAEGYTSHLSNGSSIASEQVMFAIGRHPNVANLGLEKA
                     GVSINPSNGGIAVDGWSRSSVPHIYAIGDVTHRHNLTPVAIREGHAFADTVFGKHPVQ
                     VDHSNIPTAVFSQPEVGVVGLTETEARAQFSHVDIYKATFRPIKATMSGRDTRVLMKL
                     VVDGSTDRVLGCHIVGDTAAEIIQAVAIAVKMKATKADFDATMALHPTTAEELVTMRT
                     PTARHVREAAE"
     gene            2687..3109
                     /locus_tag="VM910_18995"
     CDS             2687..3109
                     /locus_tag="VM910_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64648.1"
                     /translation="MKKTLHIVAIAVALAAFVPAASAQQADTPKQTLRAKIKEKYLER
                     RAQFMRRVKDAYFAVGCKVLAREAGILPLTSKDTYMAYIGDQTIVDLKEDEALRQAAM
                     QEGLDRAAKPGECDYYRTHPEAADAIRRAAAEAAAKPR"
     gene            complement(3263..4351)
                     /locus_tag="VM910_19000"
     CDS             complement(3263..4351)
                     /locus_tag="VM910_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI64649.1"
                     /translation="MARIETIDAGFYRIPLPVILSDSTHGEIKAFELLTLRVRDSDGA
                     EGVGYTYTVGRNGGAIADILRREIPDLIKGREADDTEAIWQHVWWGLHYGGRGGPVVL
                     ALSALDIALWDLKARRAKLPLWRLLGGFDRRVPCYAGGIDLDLSVEALLKQTDDNLSN
                     GFRAIKMKVGRPDLASDVARVRAMRKHLGDGFPLMADANMKWTVEEAIRAARALQPYD
                     LFWLEEPTIPDDIAGHARILASGGVPIAAGENLRTLWEFKSYIAAGAVSYPEPDVTNC
                     GGVTSFMKIARLAEAFNLPVTSHGAHDITVHLLAACPNRAYLEAHGFGLDRYIEDPLV
                     LEDGMALAPMRPGHGVNFDWKGLAQCAA"
     gene            complement(4431..5030)
                     /locus_tag="VM910_19005"
     CDS             complement(4431..5030)
                     /locus_tag="VM910_19005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI64650.1"
                     /translation="MPRPAEPTRKRILEGAYKLFRRRGYSRVTMDDIAAEARLTKRTL
                     YHHFTSKDQLLAAVLTAQHELALQAFRTFGDRLSGSAEAIVDGMFRELAVWADRPRWA
                     GSGFTRLVIELADLPGHPARVIARRHKARLEHCLGELLERSGVRQAAKLAREIWLLSE
                     GTVSLILVHGDQGYSVAAAAAAKTLVRAHLDKLQLSRRS"
     gene            5129..6223
                     /locus_tag="VM910_19010"
     CDS             5129..6223
                     /locus_tag="VM910_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI64651.1"
                     /translation="MPSAPLTVRGITARPVVLPLERPIVARIATITEWPVILIDLLTE
                     EGIVGRSYLEPYVVKSLHYLVPALNDLGSALKGRRVAPIELFDAARKSLHFVGYEGLS
                     MIAASGLDMAAWDALARAAKLPLCVLLGGSIGPVKSYNSNGLWLKAPAAVAEEAIQLR
                     DEGGFAGLKLRLGRDRAADDLATLDAVRKAVGDDMELMVDFNQGLDLADALRRCHMID
                     GHGLAWIEEPILYDNLDGHAQLAAELSTPIQIGENFYGPRAMHSALQKKACDLVMPDF
                     MRIGGVTGWIRAAAIAGAAGIPVSTHLYPEVAAHMMRVTETAHWLEWQDWTDPLLQRP
                     YLVKDGLLHIPDVPGVGLDWNEDAVKRYLV"
     gene            complement(6349..7251)
                     /locus_tag="VM910_19015"
     CDS             complement(6349..7251)
                     /locus_tag="VM910_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI64652.1"
                     /translation="MAKAALDDVFSDDITVPAADGYAIAATLFLPRGAKRHAILINSA
                     TAVPRKIYRGFAGYLARRGFAALTYDYRGIGGSRLRALEGYDQPRSLVGFKATMADWA
                     ALDATAMVGWMRERYRKLPLTYLGHSFGGQVLGLLGNNNEVSRALLVAAQAGYWKLMT
                     SPERYRVFAMLNFVGTPLTRLLGYAPGWSGLGEDLPRDAFLQWVRWVNSPRYLFDDKT
                     LAGLSNFPEYRGALRAICLSDDPWATRPAVELLCSGFTSIKPEILTVTPPDVGATKLG
                     HFGFFRPEHRDTLWRGAAEWLERE"
     gene            7432..8820
                     /locus_tag="VM910_19020"
     CDS             7432..8820
                     /locus_tag="VM910_19020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HVI64653.1"
                     /translation="MSERWTPESWRSRPVLQIPDYPDAKALADVEAQLATFPPLVFAG
                     EARNLKKALGRVALGDAFLLQGGDCAESFAEHGANNIRDFFRVFLQMAVVLTYAGAVP
                     VVKLGRIAGQFAKPRSSPTETVNGVELPSYRGDIVNDIAFTAQARTPDPQRQLMAYRQ
                     SAATLNLLRAFATGGFANLGSVHQWMLGFLKDSPQSRKYKELADRISEALNFMRACGL
                     DLESHPELRATDFYTSHEALLLGYEQAFTRVDSTTGDWYATSGHFIWIGDRTRQLDHG
                     HVEYFRGIKNPIGLKCGPSMKPDELIKLIDLLNPDNEPGRLTLITRFGADKVAEQLPA
                     LLRAVKREGRIVVWSCDPMHGNTVTSTTGYKTRPFDRILSEVRSFFSIHAAEGTYPGG
                     VHLEMTGKDVTECVGGARAITDEDLNDRYHTVCDPRLNAEQSIDMAFLIAELLKRERG
                     SKVKPVSAAAGF"
     gene            8822..9175
                     /locus_tag="VM910_19025"
     CDS             8822..9175
                     /locus_tag="VM910_19025"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HVI64654.1"
                     /translation="MLRLWRATINSRNGLVFAVRSEQAIREELIALLLSIPTAWLVGA
                     TVMRRVELICAVALILVVELLNTAIEKLADRLTTDRDPRIMHVKDMGSAAVGVTLLMA
                     ALFWLFAIAERMGAI"
     gene            9220..>10314
                     /locus_tag="VM910_19030"
     CDS             9220..>10314
                     /locus_tag="VM910_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVI64655.1"
                     /translation="MTERPAEFLITLAQLNPTVGDVEGNANRARAARARAAADGADLV
                     VLSELFIAGYPPEDLVLKPAFQAACRSAVEALARETADGGPAMLVGSPWVEEGKLYNA
                     CALLEGGRVSALRFKANLPNYGVFDEKRVFARGPAAGPVTVRGVRVGVPICEDVWVEE
                     SPDYENVVECQAETGAEIIVVPNGSPYARDKTDVRLSIAVARVTESGLPLIYLNQVGG
                     QDELVFDGASFALNGDLSVAAQLPDFVEDITTLRWTRGNDGWRCAGPIMPVLEGDKAD
                     YAACVLGLRDYVRKNGFPGVLLGISGGIDSAVCAAIAVDALGKEAVRGVMLPYRFTAQ
                     VSLDDAAKLANNLGVRYEVLPIADAVEGFEK"
BASE COUNT         1875 a         3200 c         3374 g         1865 t
ORIGIN      
        1 gcgcgacggc atgaagctcg ggctcggcac cggctcgacc gcaaagcact tcgtcgcgct
       61 tctgggcgag cgggtgcgcg ccgggctcaa ggtcgtcggc gtgccgacat cggaggcgac
      121 ccgtgcggat gccgagcgct gcggcattgc actgaccagc ctcgacgaga tcgatcggct
      181 cgacctgacg gtggacggcg ccgacgagat cgacccgcaa ctcaacctca tcaagggcgg
      241 cggcggcgca ctcctgcgcg aaaagatcgt ggccgcggcc tcggatcgta tgatcgtgat
      301 cgccgacgag tccaaatggg tggccgcgct cgggcgcttc cctctgccaa tcgaggtcat
      361 cccgttcggc cttgccgcca cgcagcgcgc cgttgtccag gcgtttgcgg aatgcgcggt
      421 atccgggcaa atggcggtcc gaaaggccag ggatggtcac gtttttgtca ccgatggcgg
      481 ccactggatt gtcgacgccc acctcggccg catagaggat gccccgcgtc tggcgggcct
      541 tttgggcgca atccccggtg ttgtcgagca cgggctgttc atcggtctcg ccagcacggc
      601 cattctggcg ggtgcgcaag gaattcgcgt tgttgagcgg cagtgacgcc gcagctttcc
      661 aaggagaaac ggaatgaaga gcgttttcag aattttgccg gctgcgggcc tagcgctggc
      721 tctcgctatg gctgcattgc cggctgccgc acagcaggcc gcggctccgc cgaaggccgc
      781 ctcgccgggt gcgattgcag ccgccaaaga aatcctggcg atgaagaacg cgtctgcgat
      841 gtaccagaac gccgtgccca acatcgtgca gcagaccaag gacacgctgc tgcagaccaa
      901 tctcaactat cagaaggacc tcaacgaggt cgcggtgatc gtggcgaaga atcttgccgg
      961 ccgcgaaaag gagatcggcg agggcatggc ccagatctat gccagcgagt tcaccgagca
     1021 ggagctgaag gacctcgtga ccttctacaa atccccgctc ggccagaagc tgttggccaa
     1081 cgagccgcgg gcaatccagt ccagcatggc ctatatgaac cagtgggcgc agcaattcgg
     1141 cgaaatcgtg aacggcgaat tccgcgccga aatgcgcaag cgcggcaagc aaatctgatc
     1201 gaaatggctg agttcgacgt cgacctgttt gtgattggag ggggttcggg cggggttcgc
     1261 gccgcccgga tcgcggcggg tcatggcgcg cgcgtcatgg ttgccgaaga ataccggatg
     1321 ggcggcacct gcgtgatccg cggctgcgtg ccgaaaaaac tgttcgttct cggctcgcat
     1381 gttcgtcagg agatcgagga tgccgcgggc ttcggctgga gcattccgtc ggcgacgttc
     1441 gactggccga cgctggtcgc caacaaggac aaggagatcg cacggctgga ggcggcctat
     1501 accgcaaatg tcgagaagtc gggcgcccgc atcgtcaaga cgcgcgccgt gttcgaagat
     1561 ccgcaccatt tgctgctagc gaccggcgag accgtccgcg ccagatatgt gctggtcgcg
     1621 accggcggcg cacccaacca cggccctatc atccccggcg tcgagtacgt gatctcctcg
     1681 aacgaggcgt ttcacctgcg cgagttgccg aagcggatcg tgatccaggg cggcggctat
     1741 atcgctctgg aattcgcgtg catcttcgcc ggctatggct ccgacgtcac ggtgatctat
     1801 cgcggcgaga atatcctgcg cggtttcgac gacgatgtcc gcacccatgt gcgcgccgag
     1861 ctggagaggg aaggcatcac catcctgacc ggcaacacga tcacgaaggt cgacaagcac
     1921 gccgagggtt atacgagcca tctgtcgaac ggatcgagca tcgcttccga gcaggtgatg
     1981 ttcgcgatcg gccggcatcc gaacgtcgcc aatctcggcc tggagaaggc gggcgtctcc
     2041 atcaatccga gtaatggcgg catcgccgtc gacggctggt cgcgctcctc ggtgccgcat
     2101 atctacgcga tcggcgacgt cacccatcgc cacaatctga ctcctgtcgc gatccgcgag
     2161 ggccacgcct tcgccgatac cgtgttcggc aaacatccgg tgcaggtcga tcacagcaat
     2221 attccgaccg cggtgttttc gcagccggag gtcggcgtcg tggggctcac tgagaccgag
     2281 gcgcgcgcgc agttcagtca cgtcgacatc tacaaggcca cgttccggcc gatcaaggca
     2341 acgatgtcgg gccgcgatac cagggtcttg atgaagctcg tggtggacgg ctcgaccgat
     2401 cgcgtgctcg gctgccatat cgtcggcgac acggccgccg agatcatcca ggctgttgcg
     2461 atcgccgtga agatgaaggc gaccaaggcc gactttgacg ccaccatggc gttgcatccg
     2521 accacggccg aagagctcgt caccatgcgg acgccgaccg cgcgccacgt gcgtgaggcg
     2581 gcggagtagc ggcaggagcg cttgcgatca ccgtgaatgt tgcattgcgt cttgcgaggc
     2641 ggcgaatcct tgctattgga tgacgccgat tggacgggcg gacaccatga agaaaacact
     2701 ccatattgta gcgatcgccg tggcgctggc ggcatttgtt ccggcggcga gcgcacaaca
     2761 agccgatacc ccgaaacaga ccctgcgcgc aaagatcaag gaaaaatatc tggagcggcg
     2821 cgcgcagttc atgcgtcggg tgaaggacgc ttattttgca gtaggctgca aagtgctggc
     2881 ccgcgaggcc ggtattttgc cgctcaccag caaggacacc tatatggcct atatcggcga
     2941 tcagaccatt gttgatttga aggaagacga agcgcttcgc caagccgcga tgcaggaagg
     3001 gctggatcgg gcagcaaagc ctggtgagtg cgactactat cgcacgcatc cggaggcagc
     3061 cgacgccata cgacgggcgg cggccgaagc ggcggcgaag ccgcgatagc agacgaagcg
     3121 ttcaatcacg ccgggcaagc gcggccgtgc tcgatcgatc gcgcgttggg ctgccatatt
     3181 gttttgtttg cgcgtggcgg cggtcgagct caacatttgt ccggcgcgcg cctctgcatt
     3241 gattggctgc tcgccagctc gcttacgcag cgcactgcgc cagccccttc cagtcgaaat
     3301 tgacgccatg gcccgggcgc attggcgcga gcgccatgcc gtcttcaagc accagtggat
     3361 cctcgatgta gcggtccagg ccgaacccgt gcgcttccag ataggcccgg ttcgggcagg
     3421 ccgcgagcag atgcacggtg atgtcatgcg caccgtgact ggtgacggga agattgaagg
     3481 cctcggcaag ccgcgcaatc ttcatgaaag acgtcacccc gccgcaattg gtgacgtcgg
     3541 gctctggata cgagacggcg ccggcggcga tgtagctctt gaactcccaa agcgtgcgca
     3601 ggttctcgcc ggctgcgatc ggcacgccgc ccgacgcaag gattcgggca tgacctgcga
     3661 tgtcatcggg aatggttggc tcctcgagcc agaacagatc gtaaggctgc aatgcccgcg
     3721 ccgcccggat ggcctcctcc accgtccatt tcatattggc atccgccatc agcggaaagc
     3781 cgtcgccgag atgcttgcgc atcgcgcgca cgcgcgcgac gtcggaggcg aggtcgggcc
     3841 gacccacctt catcttgatg gcgcgaaagc cgttgctcaa attgtcgtcg gtctgtttca
     3901 gtagggcctc gaccgagaga tcgaggtcga taccgccggc atagcagggc acgcggcgat
     3961 cgaagccgcc gaggagacgc cagagcggca gcttcgcgcg gcgcgccttc aggtcccaca
     4021 acgcgatgtc gagtgcggac agtgccagca ccacgggacc gccacgcccg ccgtaatgca
     4081 ggccccacca gacatgctgc cagatggcct cggtatcgtc ggcctcccgg cctttgatca
     4141 ggtcggggat ttcgcgccgg agaatgtcgg cgatcgctcc accattgcgg cccaccgtat
     4201 aggtgtaacc cacgccttcg gcaccgtcgg aatcgcggac gcgaagcgtg aggagctcga
     4261 acgctttgat ttcgccgtgt gtgctgtcgg acaatatgac gggaagggga attcggtaga
     4321 atccggcgtc gatcgtctcg atgcgtgcca tgggcatctc catctttcgt ccgaaaggtt
     4381 agatcgacga ccggtggtcc ggcaacactg gggactgcaa gtatggccgt tcacgagcgg
     4441 cgcgagagct ggagtttgtc caggtgcgcg cgaaccagcg ttttggcggc ggccgctgcg
     4501 gcgaccgaat agccctggtc gccgtggacc aggatcagcg agaccgtgcc ctccgacagc
     4561 agccagattt ctcgtgcaag ctttgctgct tgccgcaccc cggatcgctc cagcaattcg
     4621 ccaagacagt gttccagtcg ggccttgtgc cgccgcgcga tcacccgcgc cgggtgcccg
     4681 ggcaggtccg caagctcgat cacgaggcgc gtgaaccccg acccggccca gcgtggtcgg
     4741 tcggcccaca ccgcaagctc gcgaaacatt ccgtccacga tggcctcggc cgagccggac
     4801 agtctatccc cgaaagtacg gaaggcttgc agcgcaagtt cgtgctgtgc ggtgaggacg
     4861 gcggcaagga gttggtcctt gctcgtgaag tgatggtaca gcgtgcgctt ggtgagcctc
     4921 gcctcggccg caatgtcgtc catggtcacc cggctgtagc ctcgccgacg aaagagcttg
     4981 taggcccctt cgagaattcg tttgcgcgtc ggttcggcgg gacgcggcat ggctgctctc
     5041 cctcaaagta tacctgttag tgaatttaca tcactgccgg ccgtggacga aatggccgtt
     5101 gccatatgcg agcctgagga gacacgccat gccttcagct cctctgacgg tgagaggcat
     5161 cactgcgcgg ccagtcgtcc ttcccttgga gcggccgatc gtcgcgagaa ttgccacgat
     5221 caccgagtgg cccgtcatcc tgatcgatct cctgaccgaa gagggcattg tcgggcgcag
     5281 ctatctggag ccttatgtcg tcaaatccct gcactatctg gtgccggcgc tgaatgatct
     5341 cggatcggcg ctgaagggtc ggcgtgtcgc accgatcgag ctgttcgacg cggcaagaaa
     5401 atcgcttcac ttcgttggct acgaaggtct ttcgatgatt gcggcctcgg gtctcgacat
     5461 ggcggcctgg gatgccctcg ccagggctgc gaaactcccg ctttgcgtgc tgctcggcgg
     5521 ctccatcggt ccggtgaagt cttacaacag caatgggctc tggctaaagg cgcccgctgc
     5581 agtcgcagag gaagccattc aattgaggga cgaaggcggt ttcgcggggc tcaagctgcg
     5641 gctgggtcgc gaccgggccg ccgacgatct cgcgacgctc gatgcggtcc gcaaggccgt
     5701 gggcgacgac atggagctga tggtcgactt caaccagggg ctcgatctcg ccgacgcgct
     5761 gcggcgatgc cacatgatcg acggccacgg attggcctgg atcgaagaac cgatcctcta
     5821 cgacaacctt gacggccatg cgcagcttgc cgccgaactg agcacgccga tccagatcgg
     5881 ggagaatttc tatggcccgc gtgcgatgca cagtgcactt cagaagaagg cgtgcgacct
     5941 cgtcatgccg gatttcatgc gcatcggcgg cgtgaccggc tggattcgcg cggcggccat
     6001 cgccggcgca gccggcatac ctgtgtcaac tcatctctat cccgaggtcg ccgcgcacat
     6061 gatgcgcgtc accgaaactg cgcattggct ggagtggcag gactggaccg atccccttct
     6121 gcagaggcct tatctggtga aagacggcct cctgcatatc cctgatgtgc ccggtgtcgg
     6181 acttgactgg aacgaggacg cggtcaaacg ctacctcgtc tagcgaacaa cccgaccgag
     6241 tcgcccggac ggggcgaatc agtttgattt gcctcacgtg cttcgcgcgt ccggtggcga
     6301 aacggcgcga aaattgcctg cgcgccgcgc gtgagcgttt tggatcgctc actcccgctc
     6361 aagccattcg gcggcgccgc gccacagcgt gtcgcggtgc tcgggccgga agaagccgaa
     6421 atggccgagt ttcgtggcac cgacgtccgg cggcgtgacg gtgaggatct ccggtttgat
     6481 cgacgtgaag cccgagcaca acagctcgac cgccggtcga gtcgcccagg gatcgtccga
     6541 gagacagatc gcgcgcaaag cgcccctgta ttctggaaag ttcgagagac cggcaagcgt
     6601 cttgtcgtcg aagagatagc gcgggctgtt cacccagcgc acccattgca agaacgcgtc
     6661 ccgcggcagg tcttcgccga gtccgctcca gcccggcgcg tagccgagca gacgggtcaa
     6721 gggggtgccg acaaagttga gcatggcgaa aacgcggtaa cgctccggcg acgtcatcag
     6781 cttccagtag ccggcttgcg ccgcaaccag gagcgcgcgg gagacctcat tgttgttgcc
     6841 gagcaggccg agcacctggc cgccaaagga atgtccgaga taggtgaggg gcagcttgcg
     6901 atagcgctcg cgcatccatc caaccatcgc ggttgcatcg agtgccgccc agtccgccat
     6961 cgtggccttg aagccgacga gagatcgcgg ctggtcatag ccttcgagcg cccggagccg
     7021 gctgcctccg atgccgcgat agtcgtaagt cagtgccgca aagccgcggc gcgcgagata
     7081 gccggcaaag ccccgataga tcttgcgcgg cacggcggta gccgaattga tcaggatggc
     7141 atgacgcttg gcgccgcgcg gcaggaacag ggtcgccgcg atcgcgtagc catcggcagc
     7201 cggcacggtg atgtcgtcgg aaaagacgtc gtccagggca gctttggcca cggcggcttt
     7261 cctcgtggat ctcccccttc gccagcaaat gataattcgg tttttgctgc aagggcttgt
     7321 ctagagagga actttacgat ggaatccaag tggcggcggc ggaacggcat gtgtataacg
     7381 cggcccttct cttaaccgat aagtagcggt tttcgctcag gagttgacgt catgtccgag
     7441 cggtggacac ctgaaagctg gcgctcgagg ccggtgttgc aaatacctga ctaccccgat
     7501 gccaaggcgc tcgcggatgt cgaggcgcag cttgcgacct ttccgccact ggtgttcgct
     7561 ggcgaagcgc gcaacctgaa gaaggcactc ggccgtgtcg ccctgggcga tgctttcctg
     7621 ctgcagggcg gggactgcgc cgaaagcttt gccgagcatg gcgccaacaa catccgcgac
     7681 ttcttccgcg tcttcctgca gatggcggtg gtgctgacct acgccggcgc cgtgcctgtc
     7741 gtgaagctcg gccgcatcgc cgggcagttc gcaaagccgc gctcctcgcc gaccgagacg
     7801 gtgaacggcg tcgagctgcc gagctaccgc ggcgatatcg tcaacgacat cgccttcacc
     7861 gcgcaagccc gcacgccgga tccacagcgc cagttgatgg cttaccggca atcggcggcc
     7921 acgcttaatc tgctgcgcgc ctttgcgacc ggcggcttcg ccaatttggg aagcgtgcat
     7981 cagtggatgc tgggctttct taaggattcg ccgcaatcgc gcaagtacaa ggaactggcc
     8041 gaccgcatct cggaagcgct gaacttcatg cgcgcctgcg gcctcgatct cgagagccat
     8101 cccgagctgc gcgcgaccga tttctacacc agccatgaag cgctgctgct cggctatgag
     8161 caggccttca cccgcgtcga ttccacgacc ggcgactggt atgcgacgtc gggccatttc
     8221 atctggatcg gcgaccgcac gcggcagctc gaccacggac acgtcgaata ttttcgcggc
     8281 atcaagaatc cgatcggcct gaaatgcggc ccgtcgatga agccggacga gctgatcaag
     8341 ctgatcgact tgctcaatcc cgacaacgag ccgggacggc tgacgctgat tacccggttc
     8401 ggcgccgaca aggtcgccga acagttgccg gctctgcttc gcgcggtgaa acgcgaggga
     8461 cggatcgtgg tgtggtcgtg cgatccgatg cacggcaata ccgtcacctc gaccacgggc
     8521 tacaagaccc ggccgttcga ccgcatcctg tcggaggtga ggagcttctt ttcgatccac
     8581 gcggcggagg gcacctatcc cggcggggtg catctggaga tgaccggcaa ggacgtcacc
     8641 gaatgcgttg gcggggcacg cgcgattacc gacgaggatc tcaacgaccg ttatcacacg
     8701 gtctgcgatc cccgtctcaa tgccgagcaa tcgatcgaca tggcgttcct gatcgcggag
     8761 ctgctcaagc gggagcgtgg cagcaaggtc aagccggtct cggcggcggc gggattttga
     8821 tttgctgcgg ctttggcggg ccacgatcaa ctcgcgcaac ggccttgtct ttgccgttcg
     8881 ctcggaacag gcgatccgcg aggaactgat cgcactgctg ctgtcgattc cgacggcgtg
     8941 gctggtcggc gccacggtca tgcgccgtgt cgagctgatc tgtgcggtcg cgctgatcct
     9001 ggtcgtcgaa ctcctcaata ccgcgattga aaagctcgcc gaccggctga ccaccgatcg
     9061 cgatccgcgg atcatgcacg tgaaggacat ggggtcggcc gcggtcgggg tgacgctgtt
     9121 gatggccgcc ctgttctggc tatttgccat cgccgagcgc atgggcgcga tctgaagctg
     9181 gcaattgaac ttggccgcgc cgcggtgcac catctggcta tgaccgaacg ccctgcagag
     9241 tttttgatta cgctcgccca gctcaacccg acggtcggcg acgtcgaggg caacgccaac
     9301 agggcccgcg ccgcgcgcgc ccgcgcggcc gccgatggcg ccgacctggt cgtgctgtcg
     9361 gaactgttca tcgccggtta tccgcccgag gacctcgtgc tgaaacccgc gttccaggcg
     9421 gcctgccgtt cggcggtcga ggcgttggcg cgcgaaaccg ccgatggggg gcccgcgatg
     9481 ctggtcggca gcccctgggt cgaggaaggc aagctctaca acgcttgcgc cttgctggag
     9541 ggcggtcgcg tgtccgcgct tcgtttcaag gccaatttgc cgaattacgg cgtgttcgac
     9601 gaaaagcgcg tgttcgcgcg cgggcccgcc gcgggcccgg taacggtgcg cggcgttcgc
     9661 gtcggcgttc ccatctgcga ggacgtttgg gtcgaagagt ctccggatta cgaaaacgtc
     9721 gtcgagtgcc aggcggagac cggcgccgaa atcatcgtcg ttcccaacgg ctcgccctat
     9781 gcccgcgaca agaccgatgt ccggctctcg atcgcggttg cgcgcgtcac cgaaagcggg
     9841 ttgccgctga tctatctcaa tcaggtcggc ggccaggacg aactggtgtt cgacggcgct
     9901 tcgtttgcgc tgaatgggga tttgtcggtc gcggcgcaat tgcccgactt cgtcgaagac
     9961 atcaccacgc tgcgctggac caggggcaac gacggctggc ggtgcgcagg gccgatcatg
    10021 ccggttctgg aaggcgacaa ggccgactac gcggcgtgcg tgctcggctt gcgcgactat
    10081 gtgcgcaaga atggctttcc cggcgtgctg ctcggcattt ccggcggcat cgattccgca
    10141 gtttgcgcgg cgatcgcggt cgatgcgctg ggaaaggagg cggtgcgcgg cgtcatgctg
    10201 ccgtatcgct tcaccgcgca ggtctcgctg gatgatgcag cgaagctcgc aaacaatctt
    10261 ggcgtccgct acgaggtgct gccgattgcc gacgcggtcg agggtttcga gaag
//
LOCUS       DATLJT010000837         4355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1432405,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000837 DATLJT010000000
VERSION     DATLJT010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432405"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            620..2848
                     /locus_tag="VM910_19035"
     CDS             620..2848
                     /locus_tag="VM910_19035"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI64656.1"
                     /translation="MLENAVRLCEAKFGTLFKYDGEGLMIEAGLGIPQPLAEYLKQRQ
                     KFKPLAGSTMERLVETRQVVHVHDVTQVDGLSSPSARLGGARTYLGVPMLKENELVGA
                     IVIYRQEVRPFTDKQIELVKNFARQAVVAIENTRLVNELRQRTDDLSESLEQQTATSE
                     VLRVISSSPGELEPVFQTMLENATRICEAKFGNLFLYEGSAFRNVCPIGPTAYTEWFR
                     REPIVELRNQHPQIPLARVARTKEVVHVPDFTEDQTYIEGDPRIVALVESAGARSLLS
                     VPMLKEGKLIGAIVIYRQEARPFTDKQIELVKNFAAQAVIAIENTRLLNELRESLQQQ
                     TATADVLKVISRSTFDLQAVLNTLVESAVRLCLADTASINRPHGNTYQQMASFGHPPA
                     YQEFMKGFPLKLGRGSLVGRTLLEGKAVHIPDVLADPEYANSEAQKIAGFRTLLGIPL
                     LREGVPIGVLALSRSTVTPFTGRQIELATTFGDQAVIAIENVRLFDEIQDKSKQLAEA
                     SERKSQFLASMSHELRTPLNAIIGLTEMMVTNAARFGTEKAAEPLRRVNAAGTHLLSL
                     INEILDLSKIEAGKLELNPESVNLPRLIDEVVGTAGQLAEKNKNHLIVEAQGNVGALN
                     AEPMRLKQILLNLLSNACKFTKEGEVALRVRKVVADGRDWVELAVADTGIGMTAEQQT
                     KLFQDFTQADSLTARRYGGTGLGLAISRKLARMMGGDVTVTSEPGKGSVFTVRLPAGA
                     NT"
     gene            complement(3143..3505)
                     /locus_tag="VM910_19040"
     CDS             complement(3143..3505)
                     /locus_tag="VM910_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64657.1"
                     /translation="MGKTRGSHAGFRHRLPDRLVRKHPVGGASLGVELRPIDTRDPGE
                     IERAVSAFAGEPNGGLIVALGARVLLHRTLIVTLAARHRLPAVYPDRAFVAAGGLIPY
                     GSDQVDQMRRAAGRAYFH"
BASE COUNT          921 a         1367 c         1313 g          754 t
ORIGIN      
        1 gctggagcaa caggcggcaa cttcggaggt cctgtgcact atcagtaact cgcccggcga
       61 gctcgagccg gtgttccagg ctatgctggc gaacgcggcg cgcatctgcg aagccaagtt
      121 tggctcgatg tatctcaatg aaggaagcgc ctttcgcact gtcgcaatgt ataatgcacc
      181 gcctgcgctt gccgagatgc gtcggcgcaa cccggtgttc cgccccaacc cccgcatcgc
      241 gctcgcacgc gcggccgcga cgaaacagac ggttcaaatc gccgacgtcc aagccgaacc
      301 ggggtacctc gatccgctgc ccggcttcag tggcccgcag atcgtcacgc tcggcggcgc
      361 tcggaccgtg gttgccgtac cgatgctcaa ggagaacgag ctggtcggtg ccattgcaat
      421 ctatcgccaa gaggttcggc cgtttaccca caagcagatc gagctggtcc agaattttgc
      481 caaccaagcc gtcatcgcca tcgagaacac acggctgctc aatgaactgc gcgaatctct
      541 gcggcagcag acggccacat cggaggtcct cgaggtcatc agcagttctc ccggccaact
      601 ggagccggta ttcaacgcga tgttggagaa cgcggtgcgc ctttgcgagg ccaagttcgg
      661 gacattgttc aagtatgatg gcgagggcct gatgatcgaa gccgggcttg gcatcccgca
      721 gcctctcgcc gaatacctca aacaacgtca gaaatttaag cccctcgcgg gcagcaccat
      781 ggaacgtctc gttgagacgc gacaggtggt tcacgtacac gatgtcacgc aagtagatgg
      841 cctaagcagc ccctcggcaa ggcttggcgg agctcgcaca tatctcggcg tgccgatgct
      901 caaggagaac gagctcgttg gcgctatcgt catctaccga caggaagttc gtccgtttac
      961 cgacaagcag atcgaattgg tcaagaactt cgcccgccag gccgtcgtcg cgatcgagaa
     1021 cacgcgcctg gttaacgagc tgcgccagcg caccgacgac ctcagtgagt ctctggagca
     1081 gcagaccgcg acctccgaag tgttgcgcgt tatctcgagc tcgcctggcg agctagaacc
     1141 ggtgttccag acaatgctgg aaaatgccac gcgcatctgc gaggccaagt tcggcaattt
     1201 attcctgtat gagggaagtg cttttcgcaa cgtctgccct atcggtccca ccgcttacac
     1261 cgaatggttc cgacgggaac ctattgttga actacgcaat cagcatccgc agatcccgct
     1321 tgcccgcgtc gccagaacca aagaggtagt ccacgttcct gatttcaccg aggatcagac
     1381 ctatatcgaa ggtgaccccc gtattgttgc cctagtcgag tctgcgggcg cgcgaagcct
     1441 cctgagcgtg cccatgctta aggaaggaaa actgattggc gctatcgtca tctatcggca
     1501 agaagcccgc cccttcaccg acaagcagat cgagctagtc aagaatttcg ccgcgcaagc
     1561 ggtcatcgcg atcgagaaca cgcggctgct gaacgagctg cgcgaatcgc ttcagcagca
     1621 gaccgccacc gccgatgtgc tcaaggtcat cagccgatcg acctttgatt tgcaagccgt
     1681 tctgaacacg cttgttgagt cggcagtgcg gctttgcctg gccgacactg cgtcaatcaa
     1741 tcgcccccac ggcaacacct accaacaaat ggccagcttc gggcacccgc ccgcatacca
     1801 ggaattcatg aagggcttcc cgcttaaact gggacgtggc tcgcttgttg ggcgaacact
     1861 ccttgaaggc aaagccgtac atatccctga tgtcctggct gatccggaat atgctaattc
     1921 ggaagctcag aaaattgccg gatttcgtac gttgctggga attccgttgt tgcgagaagg
     1981 agtgcccatc ggggtcctcg cgctgtcgcg ttccacagtg acgccgttca caggcagaca
     2041 aatcgaactt gcgacaacct ttggcgacca ggcggtgatc gccatcgaga acgtacgtct
     2101 gttcgatgaa atccaggaca agagcaagca gctggcggaa gcaagcgagc gcaagtcgca
     2161 gttcctcgcc agcatgagcc acgagctacg cacgccgctc aatgccatca tcggcctgac
     2221 ggagatgatg gtcaccaacg cggcgcgctt cggcacggaa aaggcggcgg agccgctgcg
     2281 ccgtgtgaat gccgccggca cccacctcct gagcctcatc aacgagatcc tggacctctc
     2341 gaagatcgaa gccggcaaac ttgaactcaa tcccgagtct gtgaacctgc cccgccttat
     2401 cgacgaggtc gttggtaccg caggacagct cgccgagaag aacaagaacc acctcatcgt
     2461 cgaagcccaa gggaacgttg gcgcgctcaa cgcggagccc atgcggctca agcagattct
     2521 gctcaatctc ctaagcaatg cctgcaagtt cacgaaagag ggcgaggtcg cgctgcgggt
     2581 gcgcaaggtg gtggcggacg gacgcgactg ggtcgagctt gccgtcgcgg atactggcat
     2641 cggcatgacg gcggagcagc agacgaaact gtttcaggat ttcacccagg ccgattccct
     2701 gaccgcgcgg cgctacggcg gcaccgggct cggccttgcc atctcccgca agctcgcgcg
     2761 catgatgggc ggcgacgtga ccgtgacgag cgagccggga aagggctcgg tgttcacggt
     2821 gcgcctgccg gctggtgcga acacataaac ccattcacct gccgcgagat cactacctcg
     2881 aagaatgctt cgcctttctt gcgatagacc ccaacgcgga ccttcgccgc tccacactcg
     2941 aagaaatagg cgcgggcatc ggcgggacgg tcgcgagtag gagcgcgagc gcggtgagaa
     3001 ccgtcttcat cgtcggtcct taaatcgagc gcgccgaatt ctaccccgac cggcctccgg
     3061 aggactcaag cgcggccccg aaagcggccc cggccaaaat cctccgcgcg gaggaagtcc
     3121 ataaattgtt gatatcgctc gcttaatgga aataggccct accggccgcg cgccgcatct
     3181 ggtcgacctg gtcagagcca taggggatca agccgccggc ggcgacgaag gctcggtcgg
     3241 ggtagactgc tggaagcctg tgccgggctg ccagtgtaac gatcagagtg cggtgcagga
     3301 gcaccctcgc gcccaacgct acgatcaggc cgccatttgg ctcgcctgcg aatgcagaga
     3361 cagcgcgctc gatctcgccg gggtcccgcg tatcgattgg gcgcagctcc accccaagcg
     3421 aggcgccacc gactggatgc ttccgaacaa ggcgatctgg gagccgatgg cggaatcccg
     3481 catgactgcc gcgcgtctta cccacggcgc aatttccttg agcagctcca gcagctgagg
     3541 cgcagcaggg ccagacgagc cagccgccgc tgaatgccgt gtcgatcgag cagtcggcgg
     3601 tcttcgacgt cacgccggtc accggaaccc tgaaggaaac ggttcagaat cgacagcagc
     3661 tcgtctggcg cgccagacgc agcccgaatg aacgccggtt tgaacttacc gatatgtcgg
     3721 ttgcgctccc gcgcaaccaa gctcccggcg aggttaagat aaccttcgcc gggaacgtgt
     3781 cggcactggg ctggcgcccg gagggcgacc cgaaagtcga ggtattattc gcacccgggg
     3841 tggcgcgtcc ccgttcgagc agcaagaacg cgggcggcct aaggcgtaga ggaacacgcg
     3901 ccggtcatcg cgctggtagc gcagccggtc gaacaccggc aggccatcat cgtcgcaagc
     3961 gacggcctcg ccatctatca agcacgagac agccctgagc gagagcgcag cgcgcgcgat
     4021 cagtgggacg cacccggtcc agtcgtgccc gttgcgagtg aaaagccgca cgcccgccgc
     4081 atcgcggcgc agcaccatcc ggaaacattc tgcttgatat cgtgcagcca gcccgtagcg
     4141 gcgctcggct tcgcgactgg gctgggcagg cagggctcgc agaagtcggg gaagactacg
     4201 cgacgcacca gcatgcgcta taaataccgg cgggccaggg cgagtcgagt cggacttgcc
     4261 catgaagacg aaaacgcact tcgcctttcg catcgacatc tgggatgacg tcggcgggag
     4321 catcgtcgag cacgtcgccg gtgtggacga cttcg
//
LOCUS       DATLJT010000838         4149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1432659,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000838 DATLJT010000000
VERSION     DATLJT010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432659"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VM910_19045"
     CDS             <1..169
                     /locus_tag="VM910_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HVI64658.1"
                     /translation="TQTHVSLFDGSNCGIQLDGRPVFSVQYHPEASPGPRDSHYLFDR
                     FADLMRQKKRA"
     gene            230..760
                     /locus_tag="VM910_19050"
     CDS             230..760
                     /locus_tag="VM910_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI64659.1"
                     /translation="MPDVKARAEPLAIVFRDDGLVPNNPLPFLVYKAAVEVGRGHPEE
                     TIENLFGTNGWGDMWRNGVYDYLHYHATVHEALGVARGHARVRFGGDAGQELEIRAGD
                     VAILPAGTGHQCLSASSDFSVVGAYPPGPKMQITRPTPENHAKALKSIPRVALPPTDP
                     VMGEDGPLVRLWRGAS"
     gene            complement(852..1373)
                     /locus_tag="VM910_19055"
     CDS             complement(852..1373)
                     /locus_tag="VM910_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HVI64660.1"
                     /translation="MSKNSKVSPDLDTPTDIPSAAVDKISASLNVLLADAFALYLKTK
                     NFHWHVSGRHFHDYHLLLDEQSDAIFATTDQLAERVRKLGGTTLRSIGQVSKLQTIKD
                     NNEDYVPPRQMLRELMEDNKHIAAAMRKAHQLCDEHEDSGTAGLLETFIDETERRTWF
                     LFEASRQEGSNAA"
     gene            1493..2191
                     /locus_tag="VM910_19060"
     CDS             1493..2191
                     /locus_tag="VM910_19060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI64661.1"
                     /translation="MDDWIDYYDSTHTIYASRRHRDLHFEVIARDIIGYINSPDTVVL
                     DYACGEALSAAKVAGACAMLILAEPAPGVRGRLIARFAPNTKIRVRSLDDLRNMQERS
                     IDLVVMNSVVQYMTAEELDAALAVIKRLLKPTGRLVLGDILRPEIGMPTDVYALLRFA
                     AKHGFLKDALKGLLSTALSDYRQLRSRIGLQRYSEADIAAKLARVGLSATRAPFNIGH
                     NPWRMTFVARSTLT"
     gene            complement(2615..2947)
                     /locus_tag="VM910_19065"
     CDS             complement(2615..2947)
                     /locus_tag="VM910_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HVI64662.1"
                     /translation="MNEHARALRRRMTEAEKLMWSKLRGRRFDGIKFKRQKPIAGYIV
                     DFVALDLKLIVENDGGQHAERAAADAARTSVLEEAGYHVVRFWNHDVLRNIDGVLEAL
                     VQEFDISR"
     gene            3044..>4149
                     /gene="carB"
                     /locus_tag="VM910_19070"
     CDS             3044..>4149
                     /gene="carB"
                     /locus_tag="VM910_19070"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HVI64663.1"
                     /translation="MPKRTDISTILIIGAGPIVIGQACEFDYSGTQAVKALKEEGYRV
                     VLVNSNPATIMTDPELADATYIEPITPEIVGKIIEKERYVVPGGFALLPTMGGQTALN
                     CALSLRRLGTLDKFQVEMIGATADAIDKAEDRQLFRNAMEKIGLKTPKSRLANASALK
                     KADRDRYLADHARLSGPALEDFERQWTIGESERRKRYQEHALGEALMALSEIGLPAII
                     RPSFTMGGTGGGIAYNKEEFLDIIERGLDASPTNEVLIEESVLGWKEFEMEVVRDKRD
                     NCIIICSIENLDPMGVHTGDSITVAPALTLTDKEYQIMRDASIAVLREIGVETGGSNV
                     QFAVNPVDGRMVVIEMNPRVSRSSALASKATGFP"
BASE COUNT          769 a         1326 c         1268 g          786 t
ORIGIN      
        1 gacccaaacc catgtctcct tgttcgacgg ctccaattgc ggcatccagc tcgacggcag
       61 gccggtgttt tccgtgcagt accaccccga ggcgtcacct ggcccgcgcg actcgcacta
      121 cctgttcgac cgctttgccg acctgatgcg ccagaagaag cgggcttagg gaacgagcct
      181 cctgacaccg ggttggtgcc tgcggatcgt cgcttcgcgg agccaagcca tgcctgatgt
      241 caaagccagg gccgagccgc tcgccatcgt cttcagggat gacggcctcg tgcccaacaa
      301 tcccttgccg tttttggtct acaaggctgc agtcgaggtc ggtcgcggcc atccggagga
      361 gacgatcgaa aatctgttcg gcaccaatgg ctggggcgac atgtggcgga acggcgtcta
      421 cgactatttg cattatcacg cgaccgtgca tgaggcgctc ggtgtcgcgc gcgggcatgc
      481 ccgggtgcgc ttcggcggcg atgccggcca ggaactggag atcagagcag gcgacgtcgc
      541 gatcctccca gctggcaccg gacaccaatg tctttccgca agctctgatt tttcggtggt
      601 cggcgcctat ccgccgggcc caaaaatgca gatcacgcgg ccgacgccgg aaaaccacgc
      661 gaaagcgctg aagagcattc ctcgggtcgc gctgccgccg accgatccgg tgatgggcga
      721 agacgggccg ctggtgcggc tgtggagagg agcttcgtaa ctcccctcat tgccgacgca
      781 agcgaagcaa tccacggcca cagccgaaat tccggatcgc ttcgtcgctt gcgcttctcg
      841 caatggcggc gtcatgccgc gttgctgccc tcctgacggc tcgcctcgaa caggaaccag
      901 gtgcgccgct cggtttcgtc gatgaaggtt tcgagcaaac cggccgtgcc ggaatcctcg
      961 tgctcgtcgc agagctggtg cgccttgcgc atcgcggccg cgatgtgctt gttgtcctcc
     1021 atcaattcgc gcagcatctg gcgcggcggc acgtagtctt cgttgttgtc cttgatggtc
     1081 tgcagcttgc tgacctggcc gatcgagcgc agcgtggtgc cgccgagctt gcgaacccgc
     1141 tcggcaagct gatccgtggt cgcaaagatc gcgtccgact gctcgtccag caacagatgg
     1201 tagtcgtgga aatggcggcc gctcacgtgc caatggaagt tcttcgtctt caggtagagc
     1261 gcgaaggcgt cggccaagag cacgtttagc gacgccgaaa tcttgtcgac tgcggcggac
     1321 ggaatgtcgg tcggagtatc gagatcggga gagactttgc tgtttttgct cacgtttgca
     1381 ccttccttta ggaaccgggc aatagacagg tgttaacgga gccgttaacg catcacatca
     1441 gcgcctgttc ctgcgctcgg aaccctgatt tcgccggatc ttgaccaacg ccatggatga
     1501 ctggatcgac tattacgatt ccacgcatac gatctatgcg agcaggcggc atcgcgacct
     1561 gcatttcgag gtgatcgcgc gcgatatcat cggctacatc aactcgcccg ataccgtggt
     1621 gctggactat gcctgcgggg aagcgctttc ggccgccaag gtcgcggggg cctgcgccat
     1681 gctgatcctc gcggagcccg cacccggcgt gcgcggccgg ctgatcgcgc gctttgcgcc
     1741 caacaccaag atcagggtgc gctcgctcga cgacctcagg aacatgcaag agcgctcgat
     1801 cgatctcgtg gtgatgaact cggtcgtcca atacatgacg gcggaggagc tcgacgcggc
     1861 gctcgcggtc atcaagcggc tgctcaaacc aaccggccgt ctcgtgctcg gcgatatcct
     1921 ccgtcccgag atcggcatgc cgaccgacgt ctacgcgctg ctgcgttttg cggcgaaaca
     1981 cggctttctg aaggatgccc tgaaaggcct gctctcgacc gcgctttccg attaccggca
     2041 gttgcgctcg cgcatcgggc tgcagcgcta tagcgaggcc gacatcgcgg caaagctcgc
     2101 tcgggtcggc ttgtccgcca cgcgcgcgcc gttcaatatc ggccacaatc cgtggcgcat
     2161 gacctttgtg gcccgctcca cgctgacata gggcgttagg gttaatccgc ggaagcgttg
     2221 ggcggcttcg gtgtgatcgg cgatgatgga cgcggcccgg tggcggaaag cttcgacgcg
     2281 agggcggtgc atcgctcttt atcctggttc aagtccaggc cgggtctcca acctttgcag
     2341 ctccgccgcc tcggctgggc aagccggcgc cggcgaaggc tgctatcagg gacttgcaca
     2401 gcccaaaagt cctcaaaaca cggttgatgg ccccctaaaa ccctttccaa agctgcggaa
     2461 tctggtctaa agacgcctcg cgcgcgtttg ggtacgccgc gctcttgtct ttgcgggacg
     2521 cgcggtaagg cgcgcccttt ttttgtgcct ggattttgca gcgaatcgcg atctgccccc
     2581 tctccccagc ggggagaagg ttggggtgag gggatcacct ggagatgtca aactcttgaa
     2641 caagcgcctc aagaacgccg tcgatgtttc tcaagacatc atgattccag aagcgcacga
     2701 catgatagcc ggcttcttcg aggacgctgg tccgcgccgc gtccgcggcg gctcgctccg
     2761 catgctgtcc cccgtcgttc tcaacgatga gcttcaaatc aagcgcgaca aaatccacga
     2821 tgtagcccgc gatcggcttc tgacgtttga acttgatacc gtcgaagcgt cgcccgcgga
     2881 gcttgctcca catcagcttc tccgcctcgg tcatccggcg tcgcagcgca cgtgcatgct
     2941 cgttcatcgc gaagccccct cacccaaccc tctccccaat ggggagaggg cctcaaagcc
     3001 gttcctcgtc gcaaatcctt gtccgatcca tcattgagcc gcaatgccaa agcgaaccga
     3061 catctccacc atcctgatca tcggcgcagg tccgatcgtg atcggccagg cctgcgagtt
     3121 cgactattcg ggcacgcaag cggtcaaggc gctgaaggaa gagggctatc gcgtcgtcct
     3181 ggtcaattcc aatccggcca ccatcatgac cgacccggag ctggcggacg cgacctatat
     3241 cgagccgatc acgcccgaga tcgtcggcaa gatcatcgag aaggagcgct acgtcgttcc
     3301 cggcggattt gcgctactgc cgaccatggg cgggcagacc gcactgaact gcgcgctgtc
     3361 gctgcgcagg ctgggcacgc tggacaaatt ccaggtcgag atgatcggcg ccacggctga
     3421 tgccatcgac aaggcggagg accgccagct tttccgcaac gccatggaga agatcggcct
     3481 caagacgccg aaatcgcggc tcgccaacgc ctccgccctg aagaaagccg atcgcgacag
     3541 atatctcgcc gaccatgcgc ggctctcggg ccccgcgctg gaagatttcg aacggcagtg
     3601 gacgatcggc gagagcgagc ggcgcaagcg ctaccaggaa catgcgctcg gcgaagcgct
     3661 gatggcgctc tccgagatcg gactgcccgc gatcatccgc ccctccttca ccatgggcgg
     3721 caccggcggc ggtatcgcct acaacaagga agaatttctc gacatcatcg agcgcggcct
     3781 cgatgcctcg cccaccaacg aggtgctgat cgaggaaagc gtgctcggct ggaaagagtt
     3841 cgagatggag gtggtgcgcg acaaacgcga caactgcatc atcatctgct cgatcgagaa
     3901 cctcgatccg atgggcgtgc acaccggcga ttccatcacg gtggcgccgg cactgacctt
     3961 gaccgacaag gaataccaga tcatgcgcga cgcctcgatc gccgtgctgc gcgagatcgg
     4021 ggtcgagacc ggcggctcca acgtgcagtt cgcggtcaat ccggtcgatg gccgcatggt
     4081 cgtgatcgag atgaacccgc gcgtgtcgcg ctcctccgcg ctcgcctcga aggcgaccgg
     4141 ctttccgat
//
LOCUS       DATLJT010000839         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_284675,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000839 DATLJT010000000
VERSION     DATLJT010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284675"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VM910_19075"
     CDS             complement(<1..639)
                     /locus_tag="VM910_19075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64664.1"
                     /translation="MTPFRTLCGVITGVVVLAGTASAGPEVWPSRSMQVISPFTAGNA
                     SDTVARVVLDQVSRQIGQPFVIENRPGGGGTIGAAMVARADPDGYTILLHSSSLSSQI
                     VLHKTLPYDALRDFAPVIPFGIQPNVLITAASKGFKTVADLVAAAKARPGTLTFASAG
                     IGSASHMAAERLRLAAGINVQHIPFRGPEGLAEVMAGRVDYFFIPIVAAAPVI"
     gene            complement(1363..2067)
                     /locus_tag="VM910_19080"
     CDS             complement(1363..2067)
                     /locus_tag="VM910_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI64665.1"
                     /translation="MHRGKFVAYFRVSTDKQGKSGLGLDAQRKAVSDYLNGGHWTLAA
                     EYTEIESGKRTDNRPQLGAALAACKKLKAKLIVAKLDRLSRNVAFISTLMNSGVEFVA
                     ADMPHANKMTLQVLAVFAEHERDQISARTKQALAAAKERGVKLGGPKLAQASALGVAS
                     NRAKADQFAANTMPIIREIQASGVTSLRGVARALNARGVPSARGTPWSPVAVSNVIRR
                     SMEGAAMTPEADRMHP"
BASE COUNT          441 a          660 c          707 g          437 t
ORIGIN      
        1 gatcaccggg gccgccgcca cgatcgggat gaaaaaatag tcgacgcgtc ccgccatcac
       61 ttcggcgagc ccctccggcc cgcggaacgg gatgtgctga acattaatcc cagcggcgag
      121 ccgcagacgc tcagccgcca tgtgcgaagc ggagccgatg ccggcggaag caaaggtcaa
      181 cgttccaggt cttgccttgg ccgccgcaac gaggtcggca accgttttga agcccttcga
      241 cgccgcagtt atcaatacgt tcggctgaat tccgaacggg atcaccggcg caaaatcgcg
      301 gagtgcatcg tagggcaggg tcttgtgcag cacgatttgg gaactcagcg aagacgagtg
      361 cagcaaaatc gtgtagccgt ccggatcggc cctggcgacc atcgcagcgc cgatcgtccc
      421 tccgccgcca ggccggttct caatgacgaa gggctggccg atctgtcgcg atacctgatc
      481 gagcacgacg cgcgcgacag tatcgctggc attccctgcg gtgaacggac tgatcacctg
      541 cattgacctg ctgggccaga cctctggacc ggccgaagcc gttccggcca agaccacgac
      601 gcctgtgatg acaccgcaca aagtacgaaa tggcgtcatt tgatcctccc tggatgtcta
      661 agtcgaagtt ctgatcgact tcgataatct tactctaggg gcagccgatg cagcgtgtaa
      721 acgtgagggg tcggcgcaga cgcacctgac aagacagatc tcgttcgatt tacagagtgc
      781 tcgcctaccg cccgtgccgc taaaaacagg atttccacac atcccgctgg cagctctgca
      841 atgacgctac acgtctttcc gtgcaggcga aaggagtggg gggcgatgtc ggctcttggc
      901 attgtgtcac gagtaacgcc ggtccacacg gcggtgcgta actgtacgag agatgaaggt
      961 aggtccttcg agatcggcaa tcaggtgttg atctcaagcc accgcaagct gctgttgtca
     1021 aaagcaacgg tggtgaacgt tgcggaaaag ccgaaacgag atttcggcag gtatgattga
     1081 gagaggcgag cgcaagcgaa ccgctgatga cgtgtcgaaa gcggattaga cgatgccaaa
     1141 accggggggt tgccattcct ctgggatcag tttgggggat gtcctgagac tgcccaagcg
     1201 gcatccggca tggaggcggc gcgaaactca atctggctct tgtatgtgcg gtgggtgatc
     1261 gaaaaatttt ttctgtggga gcacaccgca tgcacgcccg aatttgctcg gtgctccgtc
     1321 ccgacgccct gagggcgagg gattaggcgc tgttacctca ggctagggat gcatacggtc
     1381 ggcttcgggc gtcattgccg cgccctccat cgagcgcctg atgacgttcg acaccgcgac
     1441 cggcgaccag ggcgtgcccc gggcgctcgg tacaccgcgg gcattgagcg cgcgagcgac
     1501 accgcgcagc gatgtcacgc cgctcgcctg tatctcgcgg atgatgggca tcgtgttcgc
     1561 ggcaaattga tctgctttag cccggtttga tgcaaccccc agcgctgaag cctgcgcgag
     1621 cttaggaccg ccgagcttca cgccgcgctc cttggctgca gcaagcgcct gcttggttcg
     1681 cgcactgatt tgatctcgct cgtgctcggc gaagaccgcg agcacctgga gcgtcatttt
     1741 gttcgcatgc ggcatgtcgg ctgcgacgaa ctcgactccg ctgttcatca gcgtggaaat
     1801 gaaggcgacg ttacgactga gccggtcgag tttggcgacg atgagcttgg ccttcagctt
     1861 cttgcaggcc gcgagcgcgg cgccgagctg cgggcgattg tcagtccgct tgccgctctc
     1921 gatctccgtg tactcggcgg cgagcgtcca gtggccgccg ttaaggtagt cggacacggc
     1981 cttacgctgc gcgtcgaggc caagcccgct cttgccctgt ttgtccgtgc tgacccggaa
     2041 gtaggcgacg aacttcccgc gatgcaacgc taagaggggt acaggccggg ggctgacatg
     2101 ccaaagaaaa tctttgtggg attgcggccg cactctgcgc catcagtctt ggcggatgcg
     2161 gattggtgca ggcgcgagac cactacgttc aatcgttgaa tgactatcgt gcctgcttgg
     2221 ccgcgaacgg cgctaacact caggc
//
LOCUS       DATLJT010000840         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1663079,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000840 DATLJT010000000
VERSION     DATLJT010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663079"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..747)
                     /locus_tag="VM910_19085"
     CDS             complement(343..747)
                     /locus_tag="VM910_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64666.1"
                     /translation="MARRAAAKRYAPDAFGLARRKDIGASAALSLHGFAAVDNTTMLA
                     LTDAQLAHLAIAATAVEERRRRGWLKDVAERIDRPPRRVASARARKARQRARQPSQLG
                     APIILPSGPLYACRRPVRRGGPRWASGLARAV"
     gene            828..1079
                     /locus_tag="VM910_19090"
     CDS             828..1079
                     /locus_tag="VM910_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64667.1"
                     /translation="MDICTVKPWFEVLVAVFALISAVFWFAAPRYNAIAASCAGLAAL
                     LQIAVLFMPVCRAFAQINNPTHYASIALAPRLALDASDG"
     gene            1107..1496
                     /locus_tag="VM910_19095"
     CDS             1107..1496
                     /locus_tag="VM910_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64668.1"
                     /translation="MSDQNRGGLTDQEKIDRAARHEEVRFLKRQQWAVATAGAVLSGA
                     FLATINNYHMTPLDRFLAVLLLAAGVWAGWFFLDDLQCGLARVRRALDPADHEAATRG
                     SPIVNLHKAILVASALVVAWAVLFKVR"
     gene            1581..2090
                     /locus_tag="VM910_19100"
     CDS             1581..2090
                     /locus_tag="VM910_19100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64669.1"
                     /translation="MTTNNSELSKLPQARMYLKAAQWGFDELLVKRYLGDPFVFHLVG
                     VLANLRAVQHALKGYDRTLSEEHKRVIDEWWNATPLTVPELHFIVTSRNLLLKAGDFR
                     GYAILSESSTGEEPNIVVTGTNYELVYYDENRDRQNLEEAIRKAIHWCDRELTALEAK
                     LPPVWSGRP"
     gene            complement(2143..2334)
                     /locus_tag="VM910_19105"
     CDS             complement(2143..2334)
                     /locus_tag="VM910_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64670.1"
                     /translation="MKALPFTDYQLELLRVLATPLAPSQRAAFLLEVARRLHGVELGD
                     GAVAEAQAQIRKASPWLQA"
BASE COUNT          476 a          762 c          751 g          496 t
ORIGIN      
        1 cttgtggtcg actgtgccgc ccatcgcgga ctaagtcctt gggacagatc tgaattgttc
       61 tgaatcgcgc tgaagccact ggccattggc agggcgatcc atctctttga aatcacatac
      121 gattaaagcc cgcctcgtgt cctcccctta ggccgagcat catgcgctac cacatcatcg
      181 aaaaagagcc tgttgtgcat cacagcaaaa ttggcctgct aaactcgatc tgagcattag
      241 aggtccgctt tgcccccgaa agcggacttc ccgatcttag aactactccc tccgccagct
      301 cttagcgaac gccgccatcg cggcctcgcg cgtcgccgtg cgtcaaactg ctcgcgcgag
      361 acccgacgcc caacgtggtc caccgcggcg caccggccgc cgacacgcgt atagtgggcc
      421 tgagggaagt atgatcggcg cgcccagctg cgacggctgg cgggcgcgct gtcgggcctt
      481 gcgggctcgg gcgctggcaa cgcgccgtgg tggccggtcg atgcgctcgg ccacatcctt
      541 gagccagccc cgacgtcggc gttcctcgac cgcggtggcg gcgatcgcga gatgtgcgag
      601 ctgggcgtcg gtgagcgcga gcatggtggt gttatccaca gccgcgaagc cgtgaaggga
      661 cagcgccgca cttgccccta tatccttgcg ccgtgcaagc ccgaaggcat ccggtgcgta
      721 acgtttcgcg gcggctcggc gtgccatcac ggttgcgccg cgcctagcag actggatgcg
      781 gacggtgacg cgccatgata gctcctagaa cagcaaggga tcacacaatg gacatctgta
      841 cggtgaaacc ttggttcgag gtactagttg ccgtctttgc actcatctcc gctgttttct
      901 ggtttgccgc tccccgatac aacgccatcg ctgcttcgtg tgcaggttta gcggccctcc
      961 tacagattgc tgtcctcttc atgccggtgt gccgtgcgtt tgcgcagatt aacaatccga
     1021 cacactacgc cagcatcgcg ctggcgccgc gcctggcgct ggatgcgagc gacgggtgaa
     1081 gatcgcttcg aatcgatagg cggatcatgt ctgaccaaaa tagaggcggg ttaactgatc
     1141 aggaaaaaat agatcgcgca gcgcggcacg aagaagtgcg gttcctgaaa aggcagcaat
     1201 gggctgtcgc aactgcaggg gccgttcttt cgggcgcgtt ccttgcgacc atcaacaatt
     1261 atcacatgac gccattggac agatttctcg ccgtgctgct tttagctgca ggagtttggg
     1321 ccggctggtt cttcctcgac gaccttcaat gtggtctcgc tagagttcga cgcgcgttgg
     1381 accccgctga ccacgaggcc gcaactcgag gcagcccgat tgtaaatcta cacaaggcga
     1441 ttttggtagc gagcgcccta gttgtcgctt gggctgtctt gttcaaggtc cgttgatagc
     1501 cccggaagca gactgaacct acgtcggcga cggttaccgc ccacgatgtg cgggcagctc
     1561 ttttatcccg gccaaccgcc atgacgacga ataacagcga actttcaaag cttccccagg
     1621 cacgaatgta tttgaaggca gcgcagtggg gcttcgacga actgcttgtg aagaggtacc
     1681 tcggcgatcc tttcgtcttt cacctggtag gggtcttggc taacctccgg gcggtgcagc
     1741 acgccttgaa gggctatgat cgcactctat cggaggagca caagcgggtc atcgacgaat
     1801 ggtggaatgc gacgccgctt accgtcccag aactgcactt catcgtcaca tcccgcaatc
     1861 tccttttgaa agccggcgac ttccgagggt acgcgatcct atccgagtcc tccacgggcg
     1921 aagaacccaa catcgtagta accggcacga actacgaact ggtctactac gacgaaaaca
     1981 gggaccgtca aaaccttgag gaagcgatcc gcaaggccat tcactggtgc gaccgagagt
     2041 tgactgcact ggaggcaaaa ttgccgccgg tatggagcgg acgaccataa gctaagccat
     2101 cgagcgcgga tcagtccgca agcatcttgc gcgcacgttc gtttacgcct gcagccaggg
     2161 tgacgccttg cggatctgcg cctgggcctc cgcgaccgcg ccgtcgccga gctcgacccc
     2221 gtgcagccgc cgggccacct ccagcaagaa cgccgcgcgc tgcgaaggtg ccaatggcgt
     2281 cgcgagtacg cgcaagagct cgagctggta gtcagtgaag ggaagtgcct tcatgggcgc
     2341 ggttctttgg gcgtgtgctc cttgccgcgt gcgatcgcgc gctcgaggcg ttcgcagaag
     2401 cgcgcgtcca tccgcatgag ctgatcgcgc ggccaggtcc cttgctgctc gtcgccgggc
     2461 tcgcatggcg cggtggcgag ttggc
//
LOCUS       DATLJT010000841         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_54571,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000841 DATLJT010000000
VERSION     DATLJT010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54571"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VM910_19110"
     CDS             complement(<1..415)
                     /locus_tag="VM910_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVI64671.1"
                     /translation="MKALVDVLSHAGVHWVGDGFPVRTVFLYEQRGQELSPFLLLDYA
                     GPHRFGPAKRPRGVGPHPHRGFETVTIVYDGEVAHRDSTGKGGVVGPGDVQWMTAGAG
                     IMHQEFHSPKFTRRGGLFRMVQLWVNLPAALKLAPP"
     gene            complement(448..909)
                     /locus_tag="VM910_19115"
     CDS             complement(448..909)
                     /locus_tag="VM910_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012490256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HVI64672.1"
                     /translation="MQEQRSTTWQSADPRNGGPAALRSGQTPHVFRRIGMVTGGSFEG
                     ERRSGEVVSGNDWQGVRKDDCTRLDVRLLLKTMDDALIVMTCQCLRSGSVEALAKLDR
                     GEAVDPAGYYFRFIALFETSAPKYDWINRIVAVGIGERLADGPLYDVFEVL"
     gene            complement(1123..1877)
                     /locus_tag="VM910_19120"
     CDS             complement(join(1123..1577,1577..1877))
                     /locus_tag="VM910_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611681.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVI64673.1"
                     /translation="MTDDEWAYFEPFLIRRGGRPPRNHRRALDAVFWQMRTGAPWRDL
                     PEELGNWNSIFRQFRRWADSGVFDVILEALVGSGACNAALQMVDATIIRAHHCAGGGK
                     GGAERNALGRSRGGYSTKINARTNAEGLPIGLVITPGQAHDVTAFPALMQEVDCDPEQ
                     LLGDKGYDSDAVRQEIADHGGEALIPTKANRKIQYTVDKAIYGLRNRIERFFNRLKNS
                     RRVATRYDKLIESFVAFVILATIRIWIRFVHGT"
     gene            2213..>2614
                     /locus_tag="VM910_19125"
     CDS             2213..>2614
                     /locus_tag="VM910_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64674.1"
                     /translation="MRMIVAAVGIAVSWCSSAWADDQPCYGMPALSVDAGAVPHLATV
                     TPGDRARFVKGSEQAGCPNGSAACAMRAFVVGGDAVVVTATAGDYACVTFTGPGPKAV
                     STSGLLLRARLGVPSPETPVNLSAAWAGTWRS"
BASE COUNT          465 a          829 c          807 g          513 t
ORIGIN      
        1 ggggcggcgc gagtttgagg gcggcgggca ggttcaccca gagctgcacc atccggaaca
       61 gtcctccgcg ccgggtgaat ttgggtgaat ggaattcttg gtgcatgatg cccgcgcccg
      121 ccgtcatcca ctggacgtcg cctggcccca caacgccgcc tttgccggta gagtctcgat
      181 gggcaacctc tccgtcatag acgattgtca ctgtctcgaa gccccgatgc gggtgtggac
      241 cgactccgcg cggccgcttt gcaggcccaa agcggtgggg tcccgcgtag tcgagcagga
      301 ggaaggggct gagctcttgg ccacgctgct cgtagagaaa cactgttcgc acaggaaagc
      361 catcgccgac ccagtgaacg ccggcatggc tcagtacgtc gactagtgcc ttcaccgagt
      421 tctccttcgc ttgttttaaa tcggacgtca caacacctcg aagacgtcgt agagcggccc
      481 gtccgccaac cgctcgccga tcccgacggc gacgatccgg ttgatccagt cgtacttggg
      541 cgcgctggtc tcgaagaggg cgatgaagcg gaagtagtag ccggccggat cgacggcttc
      601 gccgcgatcc agctttgcca gagcctccac ggaacccgag cggagacatt ggcaggtcat
      661 gacgatgagc gcatcatcca ttgtcttcag gagcagccgc acatcgagcc tggtgcagtc
      721 gtccttgcgc acaccctgcc agtcgttgcc gctgacgacc tcgccggacc ggcgctcgcc
      781 ttcgaaggag ccgcccgtca ccatgccgat gcgccggaag acgtgcgggg tctggccact
      841 acgtagagcg gcgggcccgc catttcgcgg atcagccgac tgccaagtcg ttgagcgctg
      901 ctcttgcagc gtcaggcttg aagaaaggcg aggagtgcct tcgccgacgt ggctagaaag
      961 cgtagccgcc ccagccgtgc cggaagtcgc cgcaagcatc gctcgccggt ttaactccgg
     1021 gaggccatca atcatcctct gaaacatcgg accactcctt cgccgctggc cgcatctgcc
     1081 ctttcaccaa aaggctaagc catgcccgat agtcacatta gactaggtcc cgtggacaaa
     1141 cctaatccag atccgaatgg ttgcaaggat gacaaaggcc acgaagctct caatgagttt
     1201 atcgtagcgg gttgcgacgc gtcgtgagtt tttcaagcga ttgaagaacc gctcgatgcg
     1261 gttgcgcagt ccgtagatgg ccttgtcgac ggtgtactgt attttccggt tcgccttggt
     1321 cgggatgagg gcttcgccgc cgtgatccgc aatttcttgg cgcacggcat cgctgtcgta
     1381 gcctttgtcg ccgagcaact gctcaggatc acagtccact tcctgcatca gcgccggaaa
     1441 tgcggtcaca tcatgggctt gtcccggcgt gatgacgagc ccgatcggca ggccttccgc
     1501 gttagtgcgt gcgttgatct tggtcgagta gccgccgcgc gaacggccga gcgcgttgcg
     1561 ctcggctccc cctttccgcc gccagcacag tggtgcgccc ggataatcgt cgcatcgacc
     1621 atctgcagcg cggcattaca tgcgccgctt ccaacaagcg cctccaggat gacgtcgaag
     1681 acgccgctgt cggcccagcg tcgaaattgc ctaaagatcg aattccagtt gccgagttcc
     1741 tccggcaggt cgcgccacgg cgcgcccgtc cgcatctgcc aaaaaacagc gtcaagcgcg
     1801 cggcgatggt tgcgcggcgg ccggccgcca cgtcggatca ggaaaggttc gaaatacgcc
     1861 cactcatcgt ccgtcatcag cccgcgaatc acagctgttc cccgcaaaga acagccttga
     1921 atcaattcac gatccttcag tcaattactt tgtccacggg acctagaaat ctaagagtcc
     1981 agggtagttt tcgttcagaa cttcccttgg cgacgttgcg gccagtttga aggaaacttc
     2041 tgaaccacca cacaagtggt ccgtcgccct cggtggcgca ctggacgtct tcggtatagc
     2101 gtgtgacgct catcgaatcg atcatcgtta tcggggggct cgcgtgtggg gagttgcgtc
     2161 gcgattcgga aacctgaccc ggttgtcgaa atcggcatcg aggcgtcacg cgatgcgcat
     2221 gatcgtggcc gctgtcggca tagccgtctc ctggtgttct tcggcttggg ccgatgacca
     2281 gccctgctac ggcatgccgg cgcttagcgt tgacgccggg gccgtgcccc atctcgcgac
     2341 cgttacgccg ggcgatcgcg cgcgcttcgt caagggcagc gaacaggctg gttgcccgaa
     2401 cgggagtgcc gcctgcgcga tgcgcgcctt cgtcgttggc ggcgacgcag tcgtggtcac
     2461 ggcaacggcc ggtgattacg cgtgtgtcac cttcacgggt cctgggccga aggcggtttc
     2521 cacgtccggt cttctgctgc gcgcgcggct cggagtgccg tcgccggaga ccccggtcaa
     2581 cttgtccgcg gcgtgggccg gcacgtggcg ctcc
//
LOCUS       DATLJT010000842         4775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_741289,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000842 DATLJT010000000
VERSION     DATLJT010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741289"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..920)
                     /locus_tag="VM910_19130"
     CDS             complement(27..920)
                     /locus_tag="VM910_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI64675.1"
                     /translation="MDRIDAMQAFVAVADLHGFAPAARKLKLSPSAVTRLIAALEERL
                     GARLLQRTTRSVALTDVGTRYLERARRILADVEEAERAAEGERTRPSGRLVVSAPVGF
                     GRLHVSPLMSAYLKRYPDVTVELRLEDRIVSLVEDGVDLAVRIGELADSTLVARQLGT
                     MRRIVVASPAYLKARGEPKRPAEIASHDTIQFGATSGLVDWRFVEDGKEVRASSAPRL
                     VTNSADAAIQYAAVGGGLTLVRAYQAADALKRGRLKIVLQRFELPPQPINIVYPTSRL
                     LSAKVRTFIDLAVEIADWKFG"
     gene            1046..1675
                     /locus_tag="VM910_19135"
     CDS             1046..1675
                     /locus_tag="VM910_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVI64676.1"
                     /translation="MTDAHLYSSDVAFTPAVKAIQARKGSREAYAHVEENGGWRTEIE
                     DKLAAFLANTDSFFLATASADGQPYIQHRGGPKGFIKILDKTTIAFADYAGNRQYITQ
                     GNLSENPRAHIFIMDYAHRRRVKIWGEARVVDDDPALLDSLMPKGYRARPEQVVIFKI
                     AAWDTNCPQHIPQKFDAADVAAALAQRDARISELEAELAKIRGEPKPVA"
     gene            complement(1754..2482)
                     /locus_tag="VM910_19140"
     CDS             complement(1754..2482)
                     /locus_tag="VM910_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI64677.1"
                     /translation="MITLHGFGPGFGLPEISPFVTKTEVQLKMAGLAYRKARAMPPAS
                     PKGQLPFIDDDGQVIADSTFIRAHIERKYGFDFDAGLSQHDRAQAWAFERMIEHHVYW
                     GLVGARWVDPDNFAKGPAHFFDGAPDDRRERLREDAQFRVAENYLLSGLGRHAPDEDI
                     ELALRSIFALSVQLGDKPYLMGEAPCGVDATAFGALAGILTPFFSSKLRDRAEQFENL
                     GAYVDRLMLQYFPEFSWKSQREAA"
     gene            complement(2516..2767)
                     /locus_tag="VM910_19145"
     CDS             complement(2516..2767)
                     /locus_tag="VM910_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64678.1"
                     /translation="MTVLYNVARFGSLWRVQIWACERLIALDLASGDFRLAIVALASR
                     TLNAAWNSLYENIAGRAWQGARFAERMTAADTMVAAAGR"
     gene            2870..3568
                     /locus_tag="VM910_19150"
     CDS             2870..3568
                     /locus_tag="VM910_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HVI64679.1"
                     /translation="MRRADRLFQIIQVLRRTRNPLTADAIAAELETSKRTIYRDISTL
                     IGQRVPIRGEAGMGYILEKGFDLPPLMLTPDEIEAAVLGAQWVVGHADPVLARAAEDL
                     MAKIADTVPERLRPFVLEPASRARPYWRREPDRIDMAKTRTQIHEGKKIALRYRDEQG
                     RDSERTIWPIAIGYHEAVRILAAWCELRRDFRSFRTDRVVEADYLDERYPERREALRA
                     KWRRSLVWEQPKDV"
     gene            3561..3884
                     /locus_tag="VM910_19155"
     CDS             3561..3884
                     /locus_tag="VM910_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HVI64680.1"
                     /translation="MSDADLSRGLAESPCQSCGACCAYSETWPRFSTESDAALELIPA
                     KLVNARGSGMRCEGDRCAALQGKIGVATSCSIYAVRPEVCRTCMPGDVECTTARRKFG
                     LPALT"
     gene            complement(4003..4401)
                     /locus_tag="VM910_19160"
     CDS             complement(4003..4401)
                     /locus_tag="VM910_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64681.1"
                     /translation="MISRRDVYDSESCPVREELLGEMYRASEQGLPSLVESVAPNVRA
                     MLALFCYRRSHLHSLALAIAASCSERDLVQLGGRVGATLYALSREAPVARGTTTPQGG
                     RKPITLSTKPLSSFVPIDDELDEDLEAATA"
     gene            complement(4514..>4775)
                     /locus_tag="VM910_19165"
     CDS             complement(4514..>4775)
                     /locus_tag="VM910_19165"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714365.1"
                     /note="catalyzes the cleavage of carbon-halogen bonds in
                     aliphatic compounds forming a primary alcohol and a
                     halide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HVI64682.1"
                     /translation="IPIAGEPADVVEIVAAYAKWMAENDVPKLFVNADPGAILIGPVR
                     EFCRSWKNQREVTVPGSHFIQEDSGPTIGKEIADWMTAGALR"
BASE COUNT          939 a         1512 c         1498 g          826 t
ORIGIN      
        1 tggtgccgaa gaaggagtga aggaagttat ccaaatttcc aatcggcgat ctcgaccgca
       61 agatcgatga aggtgcggac tttcgccgac aggagccgcg aagtcgggta gacgatgttg
      121 atcggctgcg gcggcagctc gaacctctgc agcacaattt tcaggcggcc ccgcttgagc
      181 gcgtccgccg cctgataggc gcgcacgagg gtcaacccgc cgccgaccgc cgcgtactga
      241 atcgcagcgt cggcgctgtt ggtgacgagc cgcggcgcgg agctcgcgcg cacctccttg
      301 ccgtcctcga cgaagcgcca gtcgacaagg ccggacgtgg cgccgaactg gatcgtgtcg
      361 tgggaggcga tctccgcggg ccgtttcggc tcgccgcgcg ccttcagata agcgggcgag
      421 gccacaacga tccgccgcat cgtgccgagc tggcgcgcca cgagcgtgga gtcggcaagt
      481 tcgccgatcc tgaccgcaag atcgacacca tcttcgacca ggctgacaat gcggtcttcc
      541 agccgcaact cgacggtgac atcaggatag cgcttcagat aggccgacat cagcgggctg
      601 acgtgcagcc ggccgaagcc gaccggcgcc gagaccacga gccgacccga tggccgcgtg
      661 cgctcgcctt cggccgcgcg ttcggcctcc tcgacatcgg caaggatgcg cctggcacgc
      721 tcgaggtaac gcgtgccgac gtcggtcagc gccaccgagc gcgtggtccg ctgcaacagc
      781 cgcgcgccga ggcgttcttc cagcgccgcg atcagccgcg tcaccgcgga cggcgacagt
      841 ttcagcttgc gcgccgccgg tgcgaagccg tgaaggtcgg ccaccgcgac gaaggcctgc
      901 atggcgtcga tccggtccat ggcattattt cataattcgc aatgatgaag tgtcaataag
      961 ccagattgtt taaattcgga aagcatccat tctatattcg tgatcacgcg cgagtccgcg
     1021 ggatcagtca tgaggaaatc ttccgatgac cgatgcgcac ctctattcga gcgacgtcgc
     1081 gttcacgccg gcggtaaagg ccatccaggc gcgcaaggga tcgcgcgagg cctatgccca
     1141 tgtcgaagaa aatggcggct ggcgcaccga gatcgaagac aaactcgcgg ctttcctcgc
     1201 caacaccgac agcttctttc ttgcgactgc gagcgccgac ggtcagccct atatccagca
     1261 tcgcggcggc cccaagggtt tcatcaagat cctggacaag accacgatcg cattcgccga
     1321 ttatgccggc aaccgccagt acatcaccca aggcaacctc tccgaaaacc cgagagcgca
     1381 catcttcatc atggactatg cgcatcggcg aagggtgaag atctggggcg aggcgcgggt
     1441 cgtcgatgac gatccggcgc tgcttgattc attgatgccc aaaggctacc gcgcgcggcc
     1501 ggagcaggtg gtgatcttca agatcgcggc ctgggacacc aactgcccgc aacatattcc
     1561 gcagaagttc gacgcggccg atgtggctgc cgcgcttgcg cagcgcgatg cgcgtatctc
     1621 ggagctggag gcggaactcg caaaaatcag gggcgagccc aaacctgtcg cctgaggtcg
     1681 cccacggcga gcaccgggcc gctgccgaca ttttcgatgc cggtgatctc ctctgcgagc
     1741 agcagggcgg ccgtcaggcc gcctcgcgtt gggatttcca cgaaaactcc gggaaatatt
     1801 gcagcatcag ccgatcgaca taggcgccga gattctcgaa ctgttccgca cggtcgcgga
     1861 gtttcgacga gaagaacggc gtcaggatgc cggcgagtgc gccgaatgcg gtggcatcga
     1921 cgccgcaagg tgcctcgccc atgagatacg gcttgtcgcc aagctgcacc gacagcgcaa
     1981 agatcgagcg gagcgcaagc tcgatatcct cgtcgggcgc atggcggccc agcccgctca
     2041 gaagatagtt ctcggcaacg cggaattgcg cgtcctcgcg cagcctctcg cggcgatcat
     2101 cgggcgcgcc gtcgaagaaa tgcgccggtc ccttggcaaa attgtccgga tcgacccagc
     2161 gcgcgccgac cagcccccaa tacacatgat gctcgatcat gcgctcgaat gcccaggcct
     2221 gggcacggtc gtgctggctc aagccggcat cgaagtcgaa gccatatttg cgttcgatat
     2281 gggcacggat gaaggtagag tcggcgatca cctgtccgtc atcgtcgatg aaggggagct
     2341 gccccttggg agaggccggc ggcatcgccc tcgccttccg gtaggcaagg ccggccatct
     2401 tgagctggac ctcggtcttg gtcacgaaag ggctgatttc gggcaggcca aaacccgggc
     2461 caaagccatg gagggtgatc atgcaaagct cccgatctgt tgaagatgcc cctgcctagc
     2521 gcccggctgc tgccaccatt gtgtcagcag ccgtcattcg ctccgcgaat cgggctccct
     2581 gccaggcccg gccggcgata ttttcgtaga gcgagttcca ggcggcgttg agcgtgcgcg
     2641 aggccaacgc aacgattgcg agacggaagt cgccggaggc taaatccagc gcgatcaagc
     2701 gctcgcaggc ccagatctgt acgcgccaga gcgatccaaa acgggcaaca ttgtagagaa
     2761 cggtcatgga cgaattccct tcctttacgg tgttgtcctc tattaggact cccctgctgc
     2821 cagcatgctg tcagcatcgg caagcccatg tttttgaacg agaaaatcca tgagacgcgc
     2881 cgaccgtcta ttccagatca ttcaggtgct gaggcggacc cgaaatcccc tgacggctga
     2941 tgccattgcg gccgaactcg aaacctcgaa gcgcacgatc tatcgcgaca tttcgaccct
     3001 gatcggccag cgcgtgccca ttcgcggcga ggccgggatg ggctacatcc tggaaaaggg
     3061 attcgatctg ccgcccttga tgctcacgcc cgacgagatc gaggcggccg tgctgggcgc
     3121 acagtgggtg gtcggccatg ccgatcccgt gctggcgcgt gccgcggaag acctcatggc
     3181 gaaaatcgcc gacacggtcc ccgaacgatt gcggcccttc gtgctggagc cggcaagtcg
     3241 cgcgcgcccg tactggcggc gggagccgga tcgaatcgac atggcgaaaa cgcgcacgca
     3301 aattcacgag ggcaagaaga tcgcgctgcg ctaccgcgac gagcaggggc gcgacagcga
     3361 acgaacgata tggccgatcg ccatcggcta tcatgaggca gtgcggattc tcgcggcctg
     3421 gtgcgagctg cgtcgcgatt tccgcagctt ccgtaccgac cgcgtggtcg aggccgacta
     3481 tctcgacgag agatatccgg agcggcgcga agcgcttcgt gccaaatggc gacgaagcct
     3541 ggtctgggag cagcccaaag atgtctgacg ccgacctgtc acgcggcctc gccgaaagcc
     3601 catgccaaag ctgcggcgcc tgctgcgcct attcggaaac ctggccgcgc ttctcgacgg
     3661 agagcgacgc cgcgctggag ctcattccgg caaagctcgt caacgcgcgc ggatcgggca
     3721 tgcgttgcga gggcgatcgc tgcgcagcgt tgcagggcaa gatcggagtt gcgacatctt
     3781 gctcgattta cgccgtgcgg ccggaagtct gccggacctg catgcccggc gatgtcgaat
     3841 gcacgacggc gcgaaggaag ttcggattgc ccgcgcttac ctagagtatg atccggaaaa
     3901 gtgggcgcct attttccctc gcgacaaacg cggaacgcgt ttgcgcgcag atcatgctca
     3961 aacaatgaat cggatggcga tgaggacaga gccttcgcgt cgtcacgccg tcgcggcttc
     4021 cagatcctcg tcgagctcgt cgtcaatcgg cacaaaagac gagagcggct tggtcgacag
     4081 cgtgatcggc tttcttccac cttgaggcgt cgtcgtgcca cgcgccaccg gcgcttcgcg
     4141 cgacagcgca tagagcgtcg caccgactcg gccgccgagc tgcaccaggt cgcgttcgct
     4201 gcagcttgcc gcgatcgcga gcgccagcga atgaagatgg ctccggcgat aacagaacag
     4261 ggccagcatg gccctcacgt tgggcgcgac gctctcgacc aaactcggca ggccctgttc
     4321 gctcgcgcga tacatttcgc cgagcaattc ttcccgcacc gggcaggatt cgctgtcata
     4381 gacatcgcgt cttgaaatca tcgtaaggcc cccctccatc agcaaggatg caacgcaatt
     4441 cgctaacgga gggttaacca gaaaatcagg ccgttgccgg aaggacttga tcgcggcgaa
     4501 tcagtctcgc gcttcagcgc agcgcgccgg ccgtcatcca gtccgcgatc tctttgccga
     4561 ttgtcgggcc tgaatcctcc tggatgaaat gcgagcccgg cacggtcacc tcgcgctggt
     4621 ttttccagct tcggcaaaat tcgcgcaccg gcccgatcag gatcgcgccg ggatcggcgt
     4681 tgacgaacag ctttgggacg tcgttttcgg ccatccattt ggcgtaggcc gcgacgatct
     4741 ccacgacgtc ggcgggctcg ccggcgattg gaatc
//
LOCUS       DATLJT010000843        10388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3501154,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000843 DATLJT010000000
VERSION     DATLJT010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501154"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VM910_19170"
     CDS             complement(<1..954)
                     /locus_tag="VM910_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64683.1"
                     /translation="MRIVTHNGLYDYGWLYADLRLNMPASERLEETGALATIVDENRP
                     SYKLTALCAWRGLPGKDETLLRQACATLDLITNKRKKFTPQSHIWQLPAQYVGPYAEG
                     DARNTLLLHESLAPVLDREGTRAAYRLECDLLPMVHAMRRRGIRIDTSAAEERRDLFL
                     RKCDAYLAQLSEKLGTNIGMDELNHSAWRAEVFDQYGIAFPRTEKGNPSFTAGNSGWM
                     PKHPHWLPQLIVKADKYNNAAVNFLETYILGHVVNGRVHAEIHPHRSDQGGTRSLRFS
                     YSSPPLQLMPAHDEELAPLIRGVFLPEESETWAKCDVSQQEF"
     gene            complement(1323..2099)
                     /locus_tag="VM910_19175"
     CDS             complement(1323..2099)
                     /locus_tag="VM910_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64684.1"
                     /translation="MSKEVTTTKKTVLELDGFNAFTNEIEGDDNVNTGSSIIQGTKLK
                     FLDPHWLIEGQTVTGMLLTAINVRNVVTKWSHDNKPLETQILAPGEKFPDFEKRNAEC
                     PQSEWRMSFGKKVGPWSGQHCLYLLDEQFICYTWASPITTIGSAIAVSELVNQIRRVR
                     RFRGENVYAVVELSHTHFPNAYKPDRERPYLRVDSWVRLGADRMANVLPPPTTAALPD
                     SGSAPAASGSAPAATQGAPADAQSVAPVTLKEEMQDESRF"
     gene            complement(2113..2511)
                     /locus_tag="VM910_19180"
     CDS             complement(2113..2511)
                     /locus_tag="VM910_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64685.1"
                     /translation="MKKRNAREQKLADDARLLRTWREWHREQLEEALAGVRRDVLERL
                     MEQLKDLRSARALVDFIAAQDWESVDADTRVIALFEINRAIAALRERQKLPPINDALP
                     GEPLTAFQIIRGIITQVSHTVERPAGASGK"
     gene            complement(2508..2843)
                     /locus_tag="VM910_19185"
     CDS             complement(2508..2843)
                     /locus_tag="VM910_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64686.1"
                     /translation="MSGARHRRKGDRIERELVERHHALCVHAERYPLSGASRFRGSSH
                     DVDIYAFGDDEAPLVAESKARRSGGGFVQLERWLGEYDILFLRRDHSDPLVLLPSRIW
                     AALLAKVGR"
     gene            complement(2840..3430)
                     /locus_tag="VM910_19190"
     CDS             complement(2840..3430)
                     /locus_tag="VM910_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64687.1"
                     /translation="MLLRSTPDRRPLAAHIWAKEEHGFYIEPEWCSERLFEVEKFNGA
                     IWDPASGIGRVAEAARRAGYRTIATDMVDRGYPRLDACVDFLCCESPRARNIVCNPPF
                     DLCDQFVRRALHLAGGKVAMIWLARRLNAARWLANTPLARAYLLTPRPSMPPGHVIAA
                     GEKPGGGTQDFCWLIFSHKHKGPPTLHWLCRDGGHQ"
     gene            complement(3738..3953)
                     /locus_tag="VM910_19195"
     CDS             complement(3738..3953)
                     /locus_tag="VM910_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64688.1"
                     /translation="MPNAESRNVFVNSRQVKQRYGDASDMWVHRRLHDKSGFPQPVYI
                     CGRRFWRLADLVDWERNRAARPEVTAA"
     gene            complement(4053..4652)
                     /locus_tag="VM910_19200"
     CDS             complement(4053..4652)
                     /locus_tag="VM910_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64689.1"
                     /translation="MTVERSTRGLLTNTPLDPTRVPELRQRIADHILFWRQFADALKE
                     AAKKPGGRDVKPTQANIVLDLKRASAHVRRQTDRVDAYLMRIEHDFAKGRAPNLTAIH
                     CLLEAALLLSIRTHQVTVVDNETAIESTLKKVQALETSQSGRSQRSNRNRRSWQAQAD
                     EIWRTHPGLSKLDVAKKIAGDDPTVNVNTIRRRINQSQK"
     gene            complement(4649..6004)
                     /locus_tag="VM910_19205"
     CDS             complement(4649..6004)
                     /locus_tag="VM910_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI64690.1"
                     /translation="MNKPLTAAAVQKYAPHPGKRRVIRDAGARSLFLVITPTGHKSWL
                     MRFRTTGGRIGKLVLGPVDVSGRELTGDPAVGQPLTLAAARQLAAEVLRQRALGHDPV
                     ADHKARKLRQRAAVREAASNTFGAAAKNFITKYSRQRVRRWKEQARLLGLQPTTDGLE
                     VIPGGLAQRWADKPLAAIDGHDIHQLVAETRERGAPGLERRSDGPTETRARAMLTCLS
                     RLFRWLVQHRRVETNPCAGVHRPEASKARGRVLNNDEIKAFWRATDGKEIRPQFGAVL
                     KLLLLTGCRLNEIAALRWEELGADGTQINLPGSRTKNHRPHVVPLVPLAQSIIAEMPR
                     IADCPYVFTTHGEAPLAIGSKIKQRLDEAMGSPEWRIHDLRRTAVTGMAELGIRPDVI
                     ELVVNHVSGSRGGIAGVYNRSELLPDRRNALERWAVQVQGLVSGEPDKVVSMARTGGR
                     E"
     gene            6407..6841
                     /locus_tag="VM910_19210"
     CDS             6407..6841
                     /locus_tag="VM910_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64691.1"
                     /translation="MVKHFDHCTIVVRDVDRAKKFFGFLGFKEAMSVVIAGEPFASYM
                     GVPGIEADHVTLVLENASPRTEIQLLRYRQPEPLPDPHIRDLYKLGVNHICFAVENIE
                     AEVAKLRTNGFKTRNEIMDFHSRKLVFIDGPEGVTIELSQWH"
     gene            complement(6938..8350)
                     /gene="iaaH"
                     /locus_tag="VM910_19215"
     CDS             complement(6938..8350)
                     /gene="iaaH"
                     /locus_tag="VM910_19215"
                     /inference="COORDINATES: protein motif:HMM:NF005688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HVI64692.1"
                     /translation="MVALFELGARRAADAVRNGEQTAEALAEALLARCAAASPLNAFI
                     SLEADRLRAAARAADQRRQRGERLGPLHGVPLALKDNIDTADFPTTGGTPALAAHRPK
                     RNAPIAQHLLDAGAIVLGKTNLQELAYGPTNNNAAFGPARNPYDLTRIPGGSSGGSAA
                     AVAARLAPAALGTDTGGSIRVPAALCGITSMRPTTLRWSQAGIIPLSHTRDTAGPMAR
                     HVADCVLLDGVITGGPTEVAPAPLKGLRLGLPRDYFWENLDAELAAILEAALARLHEA
                     GVVLVEGEVANVAALDAAAGFPIMLYETVIELERYLAEHETGLDFASLMAQVKSPSVK
                     QALASLRGADAVSEASYREALTKHRPALQETYRCYFQERGVAAMVFPTTPLPAAKIGE
                     DDTVMLNGVPVSTLSTFIRNLAPGSAAGIPGLSLPAGMTKAGLPVGMAIDAPEKSDHQ
                     LLAIGLALETVLPRLPAPPP"
     gene            8611..9453
                     /locus_tag="VM910_19220"
     CDS             8611..9453
                     /locus_tag="VM910_19220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI64693.1"
                     /translation="MTIIDSQVHAYEANTPKRPWHSVPNWPDHVTGDEMVAAMDKVGI
                     DGAIFISAFSMYRYDASYAVDVQRAHPGRFAIVKPVDPDDPAVGDIIADWKQTPGAVG
                     IRVMLTKEAKREPNDPGLDRILRAAVKHDFPVNILCWGNVDAGTALIDRHPDTRFIID
                     HLAILQPRTPPAPPQPWAELPKVLDLAKRKNAVIKVSGACTLSPEPYPFPDIWNPLAR
                     VFDAWGLDRCLWGTDWTRAYAVVNYEQAVEPFLKTDRLSDSERAMLMGGACAKAYRWS
                     PKKG"
     gene            9734..10120
                     /locus_tag="VM910_19225"
     CDS             9734..10120
                     /locus_tag="VM910_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64694.1"
                     /translation="MATDQVRVRSLYAGLYRTVTALGLLLAAAWLFALLSFYLSRTTG
                     ADWFARSGSVMALVGAAVTFRLANFYQRALATALKEGLVSVPREIELRLEPPQSYMVL
                     SYLGYLTGIVGTGIWGYGDLLIRLMS"
BASE COUNT         1867 a         3144 c         3292 g         2085 t
ORIGIN      
        1 aaattcttgc tgactgacgt cgcacttggc ccacgtctcg ctctcctccg gcagaaaaac
       61 gccacggatg agcggcgcga gttcttcgtc gtgcgctggc atcagctgca acggcggact
      121 cgagtaggag aaccgcagcg accgggtgcc gccttggtcg gaacggtgcg ggtggatctc
      181 ggcgtgcacg cgtccgttga caacgtgccc gaggatgtag gtctcaagga agttcaccgc
      241 ggcgttgttg tacttgtccg ctttgacgat tagctgcggc agccaatgcg gatgttttgg
      301 catccatccg gagtttcccg cggtgaaact cgggttgccc ttctccgttc gcgggaacgc
      361 gatgccatac tgatcaaaca cttctgcccg ccatgcgctg tggttgagct cgtccatgcc
      421 gatgttggtg ccgagctttt ccgatagctg agcgagatag gcgtcgcact tgcgcaggaa
      481 taagtcacgc cgctcttccg ctgccgaggt gtcgatgcgg atgccgcggc ggcgcattgc
      541 gtgcaccatc ggcaacagat cgcactcaag tcggtaagct gcgcgcgtac cctcacggtc
      601 gagcacgggc gctaagcttt cgtgtagcag gagcgtgttg cgggcatcgc cttcagcgta
      661 gggaccgaca tactgcgcgg gcagttgcca aatgtgggat tgcggcgtga acttcttgcg
      721 tttgttggtg atcagatcga gcgtcgcgca ggcttgccgg agcagggttt cgtctttgcc
      781 gggcagtcca cgccaagcgc atagcgcggt gagcttgtat gaaggacggt tctcgtcgac
      841 gatggtcgcc agtgcaccag tctcttcgag ccgttcagat gctggcatgt tgagtctgag
      901 atcggcgtat agccagccat aatcatagag tccgttgtgc gtgacgatgc gcacatcgga
      961 cgcgaatacg tcgcgcagcc aacgaatgac ctgttcccgg tcaaagttcg cgctgtcagg
     1021 gtggcgcacc ggaacgtaaa ttgtgcggat gtcattctcg tcgcgccagg cgacactaac
     1081 gccgcagata tagccgtcac cccatggcca tgcggagccg cggtcggcga acagtccgcc
     1141 gtctcgtgtt tcggtatcga gtgcgatgat gcctacacgc cgaaggtcgg gcaattcgct
     1201 tggcggttgc caatcgatgg acatcgcagc cactctgaaa cacggaatta aacactactg
     1261 tcggaggatc gcttcctggt gccgtgccgc cccgcccctt atgagcggca ccaagctgcg
     1321 atctagaagc ggctctcgtc ctgcatttcc tccttcagcg tgaccggtgc gacggattgt
     1381 gcatcggccg gcgcaccttg tgttgctgca ggggcactgc ctgaagctgc aggggcactg
     1441 cctgaatctg gaagcgcggc tgtagttgga ggtggcagca cattcgccat gcgatcggca
     1501 ccgagcctca cccaactatc gaccctgagg tatggccgct cgcggtccgg tttatatgcg
     1561 ttggggaagt gagtatgact caactcgacg accgcataga cattctcgcc gcgaaacctg
     1621 cgaacccgcc tgatctgatt gacgagttcg ctcaccgcga ttgccgaccc gatcgtcgtt
     1681 atcggcgatg cccaggtgta gcagatgaac tgttcgtcga gcagatacaa gcaatgctgt
     1741 ccactccagg gcccgacctt tttgccgaaa gacatacgcc actcgctctg aggacattcg
     1801 gcattgcgct tctcgaagtc gggaaacttt tcacccggcg cgagaatttg tgtctccagc
     1861 ggcttgttgt cgtgactcca cttggtcacc acgttccgca cgttgatggc ggtgagcagc
     1921 atgcccgtca cagtctgccc ttcaatcagc caatgtgggt cgagaaactt gagcttcgta
     1981 ccttggatga tcgagcttcc ggtgttgacg ttgtcgtcgc cctcgatctc gttcgtgaag
     2041 gcgttgaatc cgtccaactc aagaacggtc ttcttcgtag tcgtcacttc ttttgacatt
     2101 ttcgtcactc cactatttgc cggatgctcc ggcgggcctc tccactgtgt gggaaacttg
     2161 ggtgatgatt ccgcggatga tttggaatgc ggtcagcggt tcgcctggga gcgcgtcgtt
     2221 aatagggggt aatttctgcc gctcgcgcag cgcggcgatc gcacggttga tctcgaacag
     2281 cgcgataacg cgcgtgtcgg cgtcgacgga ctcccagtcc tgcgcggcga tgaagtcgac
     2341 gagtgcgcgg gcggagcgca ggtccttgag ctgctccatc agccgctcga gcacgtcgcg
     2401 acggacgccg gcgagcgctt cctcgagttg ttcgcggtgc cattcgcgcc aggtgcgcag
     2461 gaggcgggcg tcgtcggcga gcttttgctc tcgggcgttg cgctttttca tcgccccacc
     2521 tttgcgagca gcgcggccca tatgcgcgag ggcagcagca ccagcggatc ggagtgatcg
     2581 cggcgtagga acaagatgtc gtattcgccg agccatcgct cgagttgcac gaagccgccg
     2641 ccgctccggc gtgctttcga ttccgccacg agtggcgctt catcgtcgcc gaacgcgtag
     2701 atgtcgacgt catggctact gccgcggaat cgactggcac cgctaagcgg gtatcgttcg
     2761 gcatgaacgc aaagggcatg atggcgctcg acgagttcgc gttcgatgcg atcgcccttg
     2821 cgacgatggc gcgctccact cattggtggc ctccatcacg gcagagccaa tgcagcgttg
     2881 gcggtccttt gtgcttgtgg gaaaagatca gccagcaaaa atcctgagtt ccgcctcctg
     2941 gcttctcgcc agcagcaatc acatgccccg gcggcatcga gggtcgcggc gtcaatagat
     3001 aggcgcgggc cagcggtgtg tttgccagcc agcgcgcagc gttcagccgg cgcgcgagcc
     3061 agatcatcgc aacttttccg ccagctaggt ggagggcgcg ccgaacgaat tgatcgcaga
     3121 gatcgaacgg cggattgcag acgatgttcc gggcgcgagg agactcgcag cagagaaagt
     3181 caacgcaggc atcgaggcgc gggtagccgc gatcgaccat gtcggttgcg attgttcgat
     3241 agccggctcg gcgagcagct tcggcaacgc gaccaatacc ggatgcaggg tcccaaatgg
     3301 ctccgttgaa tttttcaact tcgaaaaggc gttcgctgca ccattctggc tcgatgtaaa
     3361 acccatgctc ttccttcgcc cagatgtggg cggccagggg acggcgatcg ggggtcgagc
     3421 gcaggagcat gggcttttct caggccacgg ccttcagctg ttcgacgtca tagcttcgac
     3481 tgtggctggc aaagcgccgt gttcggcgct cggcgtcggc aaactcgtcg cgcaaaagcg
     3541 aagtcagcgc agatcggggg cgccgcggat atgatcggca actagcgcca agaacggcgg
     3601 cgggtttcgc cttgccgcag gaacggcaag tttgctatag gggcgttgca ctgtgacttt
     3661 cctttctgcc gacgtgcagc gtcggcggct caaagaaaaa caaagcccct gggcctccca
     3721 cccaggggct tcgcaattca ggcggcggta acttcggggc gagcggctcg gttgcgctcc
     3781 cagtcaacca gatcggccag ccgccaaaag cgccgaccgc agatgtagac tggctgcgga
     3841 aagccggact tgtcatgcag ccggcggtgg acccacatgt cgctggcgtc gccgtagcgc
     3901 tgcttcacct gccggctgtt aacaaacaca ttgcggcttt cggcattcgg catcgcgtgt
     3961 ttcctttcgt tagaacacgc gaagaatggc cgttccgcgt tgtcaccgcc taacgacgat
     4021 gccaaagtta tcaacagaaa acggtgtggc ctctactttt ggctctgatt gatacgacgc
     4081 cggatggtat tgacgttcac ggttggatcg tcgccggcaa tttttttcgc cacgtccagc
     4141 ttcgacaaac cgggatgcgt ccgccaaatt tcgtcagctt gcgcttgcca gcttcggcgg
     4201 tttcggtttg atcgctgtga tctgcccgat tggctggttt caagtgcctg aacttttttc
     4261 agcgttgact cgatggcagt ctcattatca acgacagtga cctgatgagt tcggatgctg
     4321 agcagaaggg cggcttcgag aagacagtgg atggcggtca ggttaggtgc acgtcccttt
     4381 gcgaaatcgt gctcaatgcg catgaggtag gcgtccaccc tatcagtctg tctacggacg
     4441 tgcgcggagg cgcgctttag gtcgagtact atattggcct gtgtgggctt tacatcacgc
     4501 ccgcccggtt ttttggcggc ttcctttagg gcgtctgcaa actgtcgcca aaataggatg
     4561 tgatcggcga tccgctgccg cagctcaggc actcgagtgg ggtcaagcgg cgtgttagtg
     4621 agcagccctc tggtactgcg ctccacagtc attcgcgccc tcccgtgcgt gccatggaca
     4681 cgaccttgtc cggttcgccg gacacgagcc cttgcacctg cacggcccag cgctccagtg
     4741 cattgcgccg gtccggcaac agctccgacc ggttgtagac gcccgcaatg ccgccgcgtg
     4801 agccggatac gtggttgaca accagctcaa tcacgtcggg ccgaatgccc aactccgcca
     4861 tgccggtgac cgcggtgcgg cgcaaatcgt ggatccgcca ttctggcgag cccatggctt
     4921 catcgaggcg ttgtttgatc ttgctgccga tggctagcgg cgcctcgccg tgggtcgtga
     4981 acacatacgg gcagtcggcg atgcgcggca tctcggcgat gattgattgc gccagcggca
     5041 ccagcggcac cacatggggc cgatggttct tcgtcctgct accgggcagg ttgatctggg
     5101 tgccatccgc gcccagctcc tcccagcgca gggccgcgat ctcgttgagc cggcagccgg
     5161 tcaacaacag caatttcagc acagcaccga attgcggccg tatctccttg ccgtcagtcg
     5221 cccgccaaaa cgctttgatt tcatcattgt tcagcacccg gccgcgggcc ttcgatgcct
     5281 ctggccgatg caccccagca caagggttcg tctccacccg gcgatgctgc accagccacc
     5341 tgaacaagcg ggagaggcaa gtgagcatcg cccgagcccg cgtctcggtc ggaccatcac
     5401 tgcggcgttc caggccgggc gcgccgcgct cgcgcgtttc agcgaccagc tgatgaatgt
     5461 cgtggccgtc tatcgctgcc agcggcttgt cggcccagcg ctgcgccaag ccgccgggga
     5521 tgacttcgag gccgtccgtc gtcggctgca ggccgagcag ccgtgcttgc tccttccagc
     5581 ggcgcacacg ctggcgcgaa tatttggtga tgaagttctt ggcggcggcg ccgaaggtgt
     5641 tgctggcggc ctcccgcacg gcggcacgct ggcgcagctt gcgggcttta tggtcggcca
     5701 ccggatcgtg ccccagggcg cgctgccgca gcacctctgc cgccagctgg cgggcggccg
     5761 ccagggtgag cggctgcccc accgccggat cacccgtcag ctcgcggccg ctgacatcga
     5821 cggggcccag caccagcttg ccaatgcggc cgccggtggt gcggaagcgc atcagccagc
     5881 ttttgtgccc agtgggcgtg atgacaagaa acagactgcg tgcgccggca tcgcggatga
     5941 cccggcgctt gccagggtgc ggcgcgtatt tctggaccgc cgcggcggtc aacggtttgt
     6001 tcattattaa ttggtctcca gggcgcacaa gacgtgcaca ggcgcagatg ttaggcactg
     6061 ttgcctaatg ttggcaacat acgccaacaa attccggaat aacagacggt taggctgaaa
     6121 taactggggc ctctgtgaca ctatgtggcg ctgtgtgagg ccgtgccaac gcaatcataa
     6181 tccgtaggtc gccggttcga tcccggcccc cgctaccacc gcttagcgag tgggtggaaa
     6241 tgactgcata ggaatttccg atatcggacg aaatcccggg ccgtcagcgt tggaaattgt
     6301 cgacgcacct gaaaaatcga tgggaattac ggacggtcgc tcggatccga tacgtgactg
     6361 gcactcgcgg tatgatcgcg gttaggcacg cacggagtgc agacgcatgg taaaacattt
     6421 cgaccactgc acgatcgtcg tgcgcgatgt tgaccgcgcc aagaaatttt ttggattctt
     6481 ggggttcaaa gaagccatgt ccgttgtgat tgctggagag ccatttgcca gttacatggg
     6541 tgttccaggt atcgaggccg atcacgtcac gctcgtactg gagaacgcgt cgccacgaac
     6601 ggaaatccaa ctcctgcggt accggcagcc cgagccactg cccgatccgc acattcgcga
     6661 cctttacaaa cttggggtga accacatctg ttttgcggtc gaaaacatcg aggctgaagt
     6721 tgccaagctc agaacgaacg ggtttaagac cagaaacgag atcatggatt tccactcacg
     6781 aaagttggtg ttcatcgacg gccccgaagg ggtgaccata gaactctcgc agtggcacta
     6841 gctccatccg gagtacgccc ctcccgcgag gctttttcgg tcttgagatc cgtagcaatg
     6901 tggtccgacc cgcgccgagt ccgcgagcct tgatccgtca agggggcggc gcaggcaagc
     6961 ggggcagcac ggtttcgagc gcgagcccaa tcgccagaag ctggtggtcg cttttttccg
     7021 gcgcgtcgat tgccatgccg acggggagcc cggctttggt catgccggcc ggcagactaa
     7081 ggccggggat gcccgcggcg ctgccggggg cgaggttgcg gataaaggtg gacaaggtcg
     7141 acaccggcac gccgttgagc atgaccgtgt catcctcccc gatcttcgcg gctggcagcg
     7201 gcgtcgtcgg gaacaccatc gcggcgacac cacgctcctg gaaatagcaa cggtacgtct
     7261 cctgcagcgc tgggcgatgt ttggtgagcg cctcgcgata gctggcttcg gacaccgcgt
     7321 cagcgccgcg caggctcgcg agcgcttgct tgaccgacgg gctcttcact tgtgccatga
     7381 ggctcgcaaa gtcgaggccg gtttcatgct cggccagata ccgctcgagc tcgatcaccg
     7441 tctcgtaaag catgatcgga aagccggcag cggcgtcgag cgcggcgaca ttggcaacct
     7501 cgccctcgac cagcacgaca ccggcctcgt gcagtcgtgc cagcgctgct tcgagaatcg
     7561 cggcgagctc ggcgtcgaga ttttcccaga aatagtcgcg tggcagcccg aggcgcagcc
     7621 ctttgagagg agcgggagcg acctcggtcg gaccgccggt aatgacgccg tcgaggagca
     7681 cgcagtcggc aacatgacgc gccatcggtc cggcggtgtc acgggtatgc gaaagcggaa
     7741 tgatgccggc ttgcgaccag cgcaaggtgg tcggccgcat gctcgtgatc ccgcaaagcg
     7801 cggccgggac gcgaatggac ccgccggtat ccgtgccgag ggcggccggc gcgaggcgcg
     7861 ctgccacagc ggcggcgcta ccaccgctcg agccgcccgg gatgcgcgtg agatcgtacg
     7921 ggttgcgcgc cggaccaaac gccgcgttgt tgttggtcgg accataggcg agctcttgca
     7981 aattggtttt gccgagcacg atggcgccgg cgtcgagcaa gtgctgcgcg atcggtgcgt
     8041 tgcgtttcgg gcgatgcgcc gcgagtgcgg gcgttccgcc ggtggtcgga aaatcggccg
     8101 tatcgatgtt gtccttgagc gcgagcggca ccccatgcag cggccccagc cgctcgccgc
     8161 gctgacgccg ctggtcggcg gcccgtgccg cggcgcgcag gcggtccgcc tcgaggctga
     8221 tgaacgcgtt gagcggtgac gccgccgcgc agcgcgccaa cagcgcctcg gccagcgctt
     8281 ctgccgtttg ctcgccgttg cggaccgcgt ccgctgccct tcttgcaccg agttcaaaca
     8341 aagccaccat cggctcgcct tgtggtccat cgggtttggc tgcacaaagg ccatagccgg
     8401 gccgctcctg caagcttctc ccctcggtgc cccgccattt cgaatgtcgg cgattggcac
     8461 caagcgtcag gcggcgacgc cttgagacga tgtccgcttt cggagcgaag cggacagtaa
     8521 tcggctagaa tagcctaaac gacagcgagg acacggctgg cgcgacttgt tgcggtaccg
     8581 aactctccta gcaattgagg agcttccccg atgacgatca tcgattccca ggtccacgct
     8641 tatgaggcga acactccgaa gcgaccctgg cacagcgtgc cgaactggcc cgatcacgtt
     8701 acgggtgacg agatggtagc ggcgatggac aaggtcggca tcgacggcgc gatcttcatc
     8761 tccgcctttt ccatgtaccg ctacgacgcg agttatgcgg tggacgtgca acgggcccat
     8821 cccggtcggt tcgcgatcgt caagccggtc gacccggatg atccggccgt gggcgatatc
     8881 atcgccgact ggaagcagac accgggcgcg gtcggcatcc gcgtcatgct gacgaaggag
     8941 gcgaagcgcg agccgaatga cccgggcctc gaccgcatcc tgcgcgcggc cgtaaagcac
     9001 gactttccgg tcaacatcct gtgctggggc aacgtggatg cgggcacggc gctgatcgac
     9061 cgtcaccccg atacgcgctt catcatcgac catctggcta tcctgcagcc ccgcacacca
     9121 ccggccccgc cgcagccgtg ggcggagctg ccgaaggtgc tggaccttgc caagcgcaag
     9181 aacgcggtga tcaaggtgag cggtgcctgc acgctatccc cggaaccgta tccgttcccg
     9241 gacatctgga atccgctcgc ccgcgtgttc gatgcctggg gtttagaccg ctgcctgtgg
     9301 ggcacggact ggacgcgcgc atacgcggtc gtcaactacg agcaggccgt cgagcccttc
     9361 ctcaagaccg accgcctgag cgacagcgag cgagcgatgc tgatgggcgg cgcgtgcgcc
     9421 aaagcctatc gttggtcgcc gaagaaaggt taggtcgctc gctacccccg cacaggaatt
     9481 cggcgctgtc ccggcgccag tttgtagaaa tgtcacgacg gcaaaatctc aaagccgagg
     9541 cgttgcagag agtacattcg acgcacaggc tggtttggga ttcttaagct ttgcctcggt
     9601 cgccgtcatt cccgacccag cgaccgggac tttccttttt cgatcggacg tacccccgct
     9661 cggcggcgcg gacgaggtgc ggtacatttc attcccgtaa gttgggtatt cctttgacgc
     9721 tgaggcgtga aaaatggcca ccgatcaagt tagagtaaga tcgctgtacg cgggactata
     9781 ccgcaccgtt actgcattgg gtctgctgct ggcggctgcc tggttgttcg cgctgttgtc
     9841 cttttacctg tccaggacga caggcgctga ttggtttgcg cggtcaggct cggtcatggc
     9901 tttggtcggt gcggcagtca ccttcaggct tgccaatttc taccagcgtg cactcgcgac
     9961 ggcgctcaaa gaaggcctcg tgtcggtgcc cagagaaatc gagcttcgcc tcgaaccgcc
    10021 ccaatcgtat atggtccttt cgtatctcgg ctatctcact ggcatcgtgg gtactgggat
    10081 ctggggctat ggggacctgc tgatccgact gatgtcgtga gtccctctcg cgctttcagg
    10141 gggacctcga atcgaccgcg tgctgacggt gcggttgcgc gacgtaacgt tcaacggagg
    10201 aagactgcca tcgccttgca tcgtcgtcgc catgtgcgaa gctcgggtag gcgccgtatg
    10261 ggtcgccgga tcaggacgcg cgggcttgcg tcgcgagagc ggtatggatg acggcgatac
    10321 ttcaaccgtc aacgaggaga agcaaagatg ttcattgcca tgaaccgctt ccgggtgaag
    10381 aaggggtc
//
LOCUS       DATLJT010000844         4962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3271115,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000844 DATLJT010000000
VERSION     DATLJT010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3271115"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..746
                     /locus_tag="VM910_19230"
     CDS             339..746
                     /locus_tag="VM910_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HVI64695.1"
                     /translation="MVTSANPILHCFELAAERCADLTPAVYERLSREYPETQAMFRSE
                     GSDLVNGSMLTFAIEAILDFAGDRAGKFRMIECEVSSHDDYGTSRELFFAFFGVIADT
                     LRDLLGSDWSGEIDDAWNRMLDDIKSIVMAEDA"
     gene            complement(781..1128)
                     /locus_tag="VM910_19235"
     CDS             complement(781..1128)
                     /locus_tag="VM910_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64696.1"
                     /translation="MTFQRIAIALFVLLLAGLGVAFYIDWRLAAQMRTMKAFVEGYVF
                     AEAVAACEKAPSSTPFKWNSGKNTSVVGLNSVKLDKDTVIASYTLLADSVFCDYDPVR
                     RKADIGSNFLERD"
     gene            complement(1150..1425)
                     /locus_tag="VM910_19240"
     CDS             complement(1150..1425)
                     /locus_tag="VM910_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HVI64697.1"
                     /translation="MAYTISTLFAIYFVLWWVTLFVTLPFGVRSQHEEGGGVPGTDPG
                     APIASLMGRKLIWTTIVSAAIFALAMLAYNAGYLSIERLSKLMGMPF"
     gene            complement(1452..1856)
                     /gene="mce"
                     /locus_tag="VM910_19245"
     CDS             complement(1452..1856)
                     /gene="mce"
                     /locus_tag="VM910_19245"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HVI64698.1"
                     /translation="MLGRLNHVAIAVKDAEKAARIYGAAFGAEISKAVPLPEHGVITV
                     FVTLPNTKIEFIQPLGDGSPIAKFLERNSDGGIHHICYEVADIIASRDTLIKEGARVL
                     GDGEPKIGAHGKPVLFLHPKDFSGALVEIEQS"
     gene            complement(1872..3542)
                     /locus_tag="VM910_19250"
     CDS             complement(1872..3542)
                     /locus_tag="VM910_19250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HVI64699.1"
                     /translation="MARPDELTFAPLGGVGEIGMNLSIYGLGNRHQRSWLAVDLGVSF
                     GDEEHLPGIDLIMPDISFLEKERNNLVGLVLTHAHEDHFGAIIDLWPRLKCPVYATKF
                     SAALFEAKCEAERNPPKIPVTVVPSGGRVDVGPFNVEFIPVAHSIPESHALAIHTGVG
                     TVLHTGDWKIDPTPLVGPPTDERRLRELGEAGVLALIGDSTNAVRDGRSPSETEVAKT
                     IAALVKGASGRVAVTTFASNVARVKAVAAAAKAADREVVVVGRAMERVVQVARETGYL
                     EGVQSFRGPDLYGHFPPDKVLALCTGSQGEPRAALARIANDDHPQVTLNKGDCVIFSS
                     RTIPGNEKAVGAIVNGLVGQGVEVITDRTHLVHVSGHPRRDELRDIIAWTRPQILIPV
                     HGEALHLSEHARLARAAGVPRVLVCRNGDLVKLGPGDPGIIEELPAGRLYKDGTILED
                     SKSRAVIERRRMGFAGCVFVAIAMTEQGQLADDPEVDLVGIPEKNKLGDELDEIVFDV
                     VVSTVEGLPRARRRDPDGVAESVRRAVRSVVNEQWGKKPLCLVHVLEV"
     gene            complement(3542..4351)
                     /locus_tag="VM910_19255"
     CDS             complement(3542..4351)
                     /locus_tag="VM910_19255"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HVI64700.1"
                     /translation="MPFTLGPRAASRGYRVVAFERTGSTNVEAMARAKAGDPGPMWFV
                     TTEQTAGRGRRNRPWIAPRGNLASSILEVMDTTPLVAATLGFAAGLAAQAALRKVSTA
                     ALQAGSERPDFLLKWPNDVLAQGKKIVGINLEAETVAGDRLAVVVGIGTNVVGAPEGT
                     PTPAISLSALGVRSTAEEVFLALSDSWAEFHGIWNNGRGFGDIRKLWLAGAVGLGEAV
                     SVRSSNAVAIEGTFETLDEQGCMVVRTTAGNRVLITAGDVHFGLAASARTT"
     gene            complement(4356..>4962)
                     /locus_tag="VM910_19260"
     CDS             complement(4356..>4962)
                     /locus_tag="VM910_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVI64701.1"
                     /translation="SIASMALGAFAAIGQKNIKRLMAYSSIGHMGFALVGLAAGTAEG
                     AQGVLVYLAIYVTMTLGAFAIILAMKRNGQALENISDFAGLSRSNPMLAYLFAALLFS
                     LAGVPPLAGFFAKYYVFVAAMKAGLFTLAVIGVLTSVVGAYYYLAIVKVMFFDEPLTR
                     LDPMRPEVATVMAAAGLFNVLYAFYPGLLVGAASAAAKSLF"
BASE COUNT          987 a         1575 c         1462 g          938 t
ORIGIN      
        1 tttgaacatt cgttgccgaa aatgatgaca aggcgaatga gatggtctag gagtttgggc
       61 tcagacttgc cgcaaggccg ggtctggtgg tccaagtctc gggaggaacc ctgacgcgca
      121 gaagcagcgt cgccccgcca atcagaatca aatccaaaca gcgggtaaat agggtcgaca
      181 caatttttgg agcagggctt cgcgtagccc tgctcttttt ttgcgcgcca tcctgtgctg
      241 ctctggcgat tgcgaaagtg caggatttgc caatacgctc ctcttccggc cgggttgggt
      301 ggaattccaa ttggctgacc agtctttcag atggatccat ggtcacgtca gcgaatccga
      361 tccttcactg cttcgaactc gccgccgagc gctgcgcgga tctcacgccg gcagtctatg
      421 agcgcctgtc gcgcgaatat ccggaaacgc aggcgatgtt tcgcagtgaa ggcagcgacc
      481 tggtcaatgg ttcgatgctt acgttcgcca tcgaggcgat cctggacttt gcgggcgatc
      541 gagccggcaa attccgaatg atcgaatgtg aggtgtcatc gcacgacgat tacggcacgt
      601 cgcgcgaact gtttttcgcg ttcttcggcg tgatcgccga cacgttgcgc gacctgctgg
      661 ggtccgactg gtcgggcgag atcgacgacg cctggaacag gatgctcgac gacatcaaga
      721 gcatcgtcat ggcggaggat gcgtaaacct cgtgttccga acccgaaatt cgatcgcgga
      781 ttagtcgcgt tccagaaaat tcgatccgat atcggccttg cgcctgaccg gatcgtagtc
      841 gcagaacacg ctgtcggcga gcaatgtgta gctcgcgatc acggtatcct tgtcgagctt
      901 gaccgagttc aatccgacga cggaggtgtt cttgccgctg ttccatttga acggtgtcga
      961 gctcggtgcc ttttcgcagg ctgcgaccgc ttcagcgaag acatagcctt ctacgaaggc
     1021 tttcatggtg cgcatctgag ccgccagtcg ccagtcgatg tagaaagcaa ctccaagccc
     1081 tgccaacagc agaacgaaca aagcaatcgc aattctctga aatgtcatgt ctccccaccc
     1141 gttgaaccgt tagaatggca tccccatcag cttcgataat ctttcgatgg agagatagcc
     1201 ggcattatag gcgagcatgg ccagcgcgaa aattgcagcc gagacgattg ttgtccagat
     1261 cagcttgcgt cccatgaggc ttgcgatggg cgctcctgga tcggtacccg gcacacctcc
     1321 gccctcctcg tgctggctgc gcacgccgaa cggcagcgtc acgaaaagcg tgacccacca
     1381 cagaacgaag tagatcgcaa acagcgtcga gatggtgtac gccatcgctg atgcccgctc
     1441 ccggcgcgga ttcaggactg ttcgatttcg accagcgccc cggaaaaatc cttcggatgc
     1501 agaaacagga ccggcttgcc atgcgcgccg atcttgggct cgccatctcc gagcacccgc
     1561 gccccttcct tgatcagggt atcgcgcgag gcgatgatgt cggcgacctc gtagcagata
     1621 tggtggatgc cgccgtccga attgcgttcc aggaattttg cgatcggcga cccgtcgccc
     1681 aggggctgga tgaactcgat cttggtgttg ggcagcgtca cgaagacggt gataacgcca
     1741 tgctcgggca gcggcacggc cttcgagatc tcggcaccaa aggcggcgcc gtagatcctg
     1801 gccgccttct cggcgtcctt taccgcaatc gcgacatgat tgagccggcc cagcatcgcc
     1861 tcttcctcca ctcagacttc cagaacgtgc accagacaga gcggcttctt gccccactgc
     1921 tcgttcacga ccgatcggac cgcccggcgc accgattccg cgaccccgtc gggatcgcgt
     1981 cgccgggcgc gcggcaggcc ctcgaccgtc gagaccacca catcgaacac gatctcgtcg
     2041 agttcatcgc caagcttatt cttctcgggg atgccgacaa gatcaacttc gggatcgtcg
     2101 gcgagttgtc cctgctcggt catcgcgata gcgacgaaga cgcaacctgc aaatcccatc
     2161 cgccgccgct cgatcacggc gcgcgacttg gaatcttcaa ggatcgtacc gtccttgtag
     2221 agccggccgg ccggcagttc ctcgatgata ccggggtcgc cgggaccaag tttgaccaga
     2281 tcgccgttgc gacagaccag aactcttgga acgccggcgg cgcgagcgag ccttgcatgc
     2341 tcagacagat gcagcgcctc gccgtggacg gggatcagaa tttgcggacg cgtccaggcg
     2401 ataatgtcgc gcaattcgtc gcggcgcgga tggccggaga catggaccag atgggttcga
     2461 tcggtgatga cctctacccc ctgtccaacg aggccgttga cgatggcgcc gacggccttc
     2521 tcgttgcccg ggatggtgcg cgaggaaaag atcacgcaat cgcccttgtt caaggtcacc
     2581 tgcggatggt cgtcattggc gatgcgtgcc agcgctgcgc gcggctcgcc ctggctgcct
     2641 gtgcagagcg ccagcacctt gtcgggcgga aagtgcccgt atagatcggg cccgcggaaa
     2701 ctctgaacgc cctcgagata gccggtctcg cgcgccacct gcaccacgcg ctccatcgcg
     2761 cgcccgacca cgacgacctc ccgatcggcg gcctttgcgg cggcggcgac cgcctttacg
     2821 cgcgcgacgt tggaggcgaa tgtagtcacg gccacccgcc cactggcgcc cttgacgagc
     2881 gcggcgatgg tcttggccac ttcggtttcc gatggcgagc gcccgtcccg caccgcattg
     2941 gtggaatcgc cgatcaatgc cagcacgccc gcctcgccca attcgcgcag ccgccgctcg
     3001 tcggtcggag gtccgaccag cggggtcgga tcgatcttcc agtcgccggt atgcagcacc
     3061 gtgccgactc cggtgtggat cgccagagca tgagattccg gtattgaatg cgcaaccgga
     3121 atgaactcga cattgaacgg cccgacgtcg acgcgcccgc cggacggcac gacggtgacc
     3181 ggaattttcg gcggattgcg ttcggcctcg catttggctt cgaacaaggc agcactgaac
     3241 ttggtcgcat agaccggaca tttcagtctc ggccagagat cgatgatggc gccgaaatgg
     3301 tcctcatgag cgtgcgtcag cacgagccca accagattat ttcgctcttt ttcaagaaag
     3361 ctgatgtccg gcatgatcag gtcgatgccg ggcaggtgct cctcatcgcc gaacgagacg
     3421 cccagatcta cggcaagcca ggaccgctgg tggcgattgc cgaggccgta gatcgacagg
     3481 ttcattccga tctcgccgac cccgccgagc ggcgcaaagg tcaattcatc cggccgcgcc
     3541 atcaggtcgt ccttgctgat gcggccaggc cgaaatggac gtcgccggcg gtaatcaaaa
     3601 cccggttccc tgccgtggtg cgcacgacca tgcagccttg ttcatcgaga gtctcgaacg
     3661 tgccttcaat tgcaacggcg ttgctcgatc tgaccgacac agcctctccg agccccacgg
     3721 ctccggctaa ccacaatttt cggatgtcgc cgaagccgcg gccgttgttc cagatgccgt
     3781 gaaactcggc ccaactgtct gacagtgcca gaaacacctc ctccgcggtc gaccgaacac
     3841 cgagagccga cagagaaatt gctggggtcg gcgttccctc cggcgctccg acgacatttg
     3901 tgccaattcc gacgaccacc gcgagccgat cgccggccac cgtctccgcc tcgaggttga
     3961 tgccgacgat cttctttcct tgcgcgagga cgtcatttgg ccacttcagc agaaaatccg
     4021 gccgttccga tcccgcttgc aacgccgccg tgctgacctt tcggagcgct gcctgcgcag
     4081 caagcccggc agcaaatccc agcgtcgccg ccaccagcgg cgtcgtatcc atcacttcaa
     4141 gaatactgct ggcaagattg ccgcgcggcg ctatccacgg ccgattacgc cttccgcgtc
     4201 cggccgtttg ctccgtggtc acgaaccaca tcggcccggg gtcgcctgct tttgcgcgcg
     4261 ccatggcctc cacattggtc gaaccggtcc gttcgaacgc cacaacccga taacctctgg
     4321 acgcagcgcg cggacctagc gtgaatggca tctatctaga atagcgattt cgccgcagcc
     4381 gacgcggcgc caacaaggag accaggataa aaagcgtaga ggacgttgaa gaggccagcg
     4441 gccgccatca ccgtggcgac ctccggccgc atcggatcga gcctggtcag tggctcgtca
     4501 aaaaacatca ctttgacgat agcgaggtag tagtatgcgc ccaccacgct ggtcaacaca
     4561 ccgatcacgg cgagcgtaaa caggccagcc ttcatcgcgg cgacgaagac gtagtatttg
     4621 gcgaagaagc cggcgagcgg cgggacgccg gccagcgaga acaggagtgc cgcgaaaaga
     4681 taggcgagca ttggattgct gcgcgagagg cccgcgaaat cgctgatgtt ctccagcgcc
     4741 tggccgtttc gcttcattgc caggatgatg gcaaaagcgc cgagcgtcat cgtcacatag
     4801 atcgcaagat aaaccagtac gccctgcgcg ccctccgccg taccggcggc aagacccacc
     4861 agcgcaaagc ccatgtggcc gatcgacgaa taggccatca gccgcttgat attcttctga
     4921 ccgatcgcgg cgaatgctcc cagcgccatc gaggcgatcg ag
//
LOCUS       DATLJT010000845         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2812061,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000845 DATLJT010000000
VERSION     DATLJT010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2812061"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VM910_19265"
     CDS             complement(<1..422)
                     /locus_tag="VM910_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64702.1"
                     /translation="MDVFFKSKAPEKDRNAAAKTAEVIERMVQQQHSVVEQVAASQPE
                     PALRADEIKEVEMLNSTKSGVPENDKNATAKAAEVMGRTVQPHDQVWARSEPTKAGTA
                     SCIGSDMSIVGNIECEGPAQVFGRIEGEVRASDLQISDG"
     gene            complement(732..1076)
                     /locus_tag="VM910_19270"
     CDS             complement(732..1076)
                     /locus_tag="VM910_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64703.1"
                     /translation="MLFGSRRTPPGFIGPCLPSPAERPPSGPGWIHEIKHDGFRMMVR
                     RDAAGVRLLTRNGQDWADRYPRIAEAAGALGVRSCMIDGEAVACDEDGMPVFDRLRRA
                     PRGATPPHRRAA"
     gene            complement(1192..1527)
                     /locus_tag="VM910_19275"
     CDS             complement(1192..1527)
                     /locus_tag="VM910_19275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64704.1"
                     /translation="MSPASFMPEQDLHFVLCDFGRSGRAYVETDPSQADASTIVRNLL
                     EGQYTQPVRVLALNVEEGWVRDVSRVIAAKVQDVARHNGQGVTRGTRDFIEAHGDPAD
                     QQQVLPLWQ"
     gene            1749..2729
                     /locus_tag="VM910_19280"
     CDS             1749..2729
                     /locus_tag="VM910_19280"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64705.1"
                     /translation="MTVTIGRRELLAALGFAAAAWSFAARAQQGRLPRIGALVLTSVD
                     AQAFGKELRDGLRELGYVEGQNFALEVRSAEGNADRLPQLAAELVSLRVDVIMAVYTP
                     CALAAKRATTTIPIVMAAVADPVGGGLVQSLARPGGNITGFSNMAAETAGKSVELFRD
                     MLPSLRRVAVLANPVNPFTKSFLEQIHLAGRTGGIEIAPVEMVRGPDEIEAAFADMAS
                     KRAQAVVVQGIFFSKAIADLAIKYRLPSAAVLRSYPHAGGLMSYGADLSHLFRRSAVR
                     AHKILQGTKPADLPVEQPTQFHLAINLKTAKALGLTIPESFLLRADEVIE"
     gene            2841..>3200
                     /locus_tag="VM910_19285"
     CDS             2841..>3200
                     /locus_tag="VM910_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64706.1"
                     /translation="MAGDPGFWDVAWNWPLPFVTVVAVLAVPMAGIIWFICQLRYKQQ
                     IATLKDRVELVDERLGDANEKIGKAEEEKTALHTTVKELNAQIEKLKKRKAQLPRELQ
                     SIVDRLAMSSATASNQVS"
BASE COUNT          588 a          983 c         1003 g          626 t
ORIGIN      
        1 ccatcgctga tctggagatc cgaggcgcgc acctcgcctt cgattcggcc gaaaacctga
       61 gctggacctt cgcattcgat gttgccgacg atcgacatgt cggagccgat gcaagaagcc
      121 gttcccgctt ttgtcggctc tgacctcgcc cagacctggt catgcggctg caccgtcctc
      181 cccatgactt cggccgcttt tgccgtcgca ttcttatcgt tctcgggtac gccgctcttc
      241 gtactattca acatctccac ctcctttatt tcgtcggcac gcagtgcagg ctcgggctgg
      301 ctcgccgcga cctgttcaac gaccgagtgt tgctgctgca ccatcctctc gatgacttcg
      361 gccgtttttg ctgccgcgtt cctatccttc tcaggtgcct tgcttttgaa gaacacgtcc
      421 acctcctttt tcgccggcgc agacggccaa ctgcaaccgt cccaggcggt gcctctaaag
      481 cggtgcatgc ttcgagcgaa gcaagatccg tggaagatgc gcgatcgccc caaggttaga
      541 attttaatat acggcgagtt agggtagttg acgaactaaa attagtgttg gtcgtcgctc
      601 gtcgatgtgc agatagcaat cgctattgct ttaacgtggg agtgtggcaa cgacctttgc
      661 tgaggcccgc agcgcggtgt gctcgactgg cggcctgatg aataaatttc cgggcgttgc
      721 gcgcgatttc atcacgctgc tcggcggtgc ggcggcgtgg cccctcgtgg cgcgcggcgc
      781 agccggtcga acaccggcat accgtcctcg tcgcaggcga cggcctctcc gtcgatcatg
      841 cacgagcgca ccccgagcgc gccggcggcc tcggcgatgc gcgggtagcg gtccgcccag
      901 tcctgcccgt tgcgggtaag aaggcggacg ccggcggcgt cgcgtcgcac catcatgcga
      961 aagccgtcgt gcttgatttc atgaatccac ccggggccgg acggcggtcg ctcggcgggg
     1021 gatgggagac aggggccaat gaagccggga ggcgtccggc ggctaccaaa caacatcgcg
     1081 gaaaccaaat agctggccgc ggtaggggag tcgatcgcta actttacggc gacccaccgc
     1141 cgacgtgcag cagcttgcgc gtcgtgagcg cgtgcgcacc ggccgcgcca atcactgcca
     1201 aaggggcagc acctgttgct gatcggcggg gtcgccgtgc gcctcaatga aatcgcgggt
     1261 cccgcgggtc accccttgcc cattgtgccg cgccacgtct tgcaccttgg ctgcgatgac
     1321 ccgcgacacg tctcggaccc aaccttcctc cacattgagc gccaggaccc taacaggttg
     1381 agtgtactgc ccttccagga gattgcggac gatggtcgaa gcgtctgctt gggatgggtc
     1441 ggtctcaacg taggcccgac cggagcgacc gaagtcgcag aggacgaagt gcaggtcttg
     1501 ttctggcatg aaagatgccg ggctcatgac gcggtactct cggcttcgag gaaaccagag
     1561 ctaagtctgt tctcaatcct ggaggccagt caacagcggc gccctataca ggttgtcgcc
     1621 gacgaagcgg ctggttgaag ccgacgacaa ggggtggaag caaccagcgt cttgccccct
     1681 tgaacgtgaa gactgcacgc ccatggcgtg ctattgttcc ctttacgcgc ctcacgcggg
     1741 aggggcgtat gacagtcacc atcggacggc gggaattgct ggccgcactc ggcttcgcgg
     1801 cggccgcgtg gtcgttcgcg gcgcgcgcgc agcagggacg gttgccgcgt atcggcgcgc
     1861 tcgtgttgac gagcgtggat gcgcaggctt tcggcaagga attgagagac ggacttcgcg
     1921 agcttggcta cgtcgaagga cagaattttg ccctcgaggt ccgttccgcg gagggaaacg
     1981 ctgatcggct gccgcagttg gcggcggagc tggtcagcct ccgggttgat gtcatcatgg
     2041 cggtctacac gccctgcgcg ctggcggcga agcgggcgac gacgaccatt ccgatcgtca
     2101 tggcggcggt cgctgatccg gtcggcggcg ggctcgtgca gagtctggcg cggcccggcg
     2161 ggaacatcac cggtttctcc aatatggccg ccgagaccgc tggaaaaagc gtcgaattgt
     2221 ttcgcgacat gctgccgtcg ctgcgccgtg tggcggttct ggccaatccg gtcaatccat
     2281 tcaccaaatc ctttctcgag cagatccatc tcgccggccg caccggcggc atcgagatcg
     2341 cgccggtcga aatggtccgc ggccccgatg aaatcgaggc tgcgttcgcg gacatggcga
     2401 gcaagcgggc gcaagccgtc gtcgtgcaag gcattttctt ttccaaggcc attgccgacc
     2461 tcgcgatcaa atatcgtctg ccgtctgccg cggtgctccg ctcatatccg catgccggcg
     2521 gcctgatgtc ctacggcgcc gacctatcgc acctgtttcg ccgcagcgcc gtccgggcgc
     2581 acaagattct gcagggcacc aagcccgcgg acctgccggt cgagcaacct acgcagtttc
     2641 atctcgcgat caacctcaag acagcgaagg cactcggcct cacgatcccg gagtcgttct
     2701 tgctgcgcgc cgacgaggtc atcgaatgaa aaggcgcgag ttcatcaccc tgctcggcgg
     2761 cgcggcggcc acgtggccgc tcgcggcgcg cgcgcaacag ccggcgctcc ccgtgaccga
     2821 gtaggaatgg ggcgatagtc atggccgggg atcctggctt ctgggatgtg gcatggaatt
     2881 ggccccttcc atttgtgacg gtcgtagcag tgctcgccgt gccgatggcc gggataatat
     2941 ggttcatttg ccaacttaga tacaaacagc agattgcaac gttgaaagat cgcgttgaac
     3001 tcgttgacga aagattaggt gatgctaacg agaaaatcgg aaaggccgaa gaagagaaga
     3061 ccgccctcca cacgacggta aaggagttga atgcacagat cgaaaagctg aaaaaacgca
     3121 aggcacaatt gccacgcgag ttgcagtcta tcgtcgatcg tctagctatg tcgtccgcaa
     3181 cggcctcaaa tcaggtaagc
//
LOCUS       DATLJT010000846         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_514279,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000846 DATLJT010000000
VERSION     DATLJT010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514279"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VM910_19290"
     CDS             complement(<1..513)
                     /locus_tag="VM910_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64707.1"
                     /translation="MTRRHSSALVAVLACASLLATSHYAAARTTHGGKGSSATTAKAA
                     GKTAKSGGKKAAARKSKDDKSASKTAVPQIPLAPGPTPETSAAEIAHVKSAIEALRHG
                     GASKATEAAAAISDPVARKLVEWTILRSDHSGGDSKRFLAFIAANPNWPSLGMFRKRA
                     EAMLWNENPKP"
     gene            861..1214
                     /locus_tag="VM910_19295"
     CDS             861..1214
                     /locus_tag="VM910_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64708.1"
                     /translation="MPDVRRREFILLPTGSRPPGRARRTRQSPRGYRGAAAATHLGRL
                     TAALAATILFGSLLSSAAEARILLCWWEYPGRMCTIIKSAKSTMRPHWAGYGYRRAPP
                     PCPRGFWAVCGECPC"
     gene            complement(1322..1639)
                     /locus_tag="VM910_19300"
     CDS             complement(1322..1639)
                     /locus_tag="VM910_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64709.1"
                     /translation="MSELVQFLCKGDHPIEARLREKTREALKESIANGHVRIKFTDTR
                     GGTELGVEIDRSRSDLRAIESDNGSAEIRVVGDLTLDYVQVSCVARIDLATLEGEGHL
                     ELR"
     gene            complement(1754..1975)
                     /locus_tag="VM910_19305"
     CDS             complement(1754..1975)
                     /locus_tag="VM910_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64710.1"
                     /translation="MALVVVSIYLASVLFCVGTTAWILRAWTRRRYWHKSPSDLHYVV
                     AGTALLLSAGIGVALASQIVVALGYPPAP"
     gene            complement(2059..2769)
                     /locus_tag="VM910_19310"
     CDS             complement(2059..2769)
                     /locus_tag="VM910_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI64711.1"
                     /translation="MTDIDILSVERLDFQVFPLPWPFAADRRTEIEDYFTHLRREKPQ
                     LWNGRVLLLRDFEISGATLQGTMFETDFASFIAWRDWGFPDRDVHACFAMGALLSRDD
                     AFLLGVMNAHTAGAGGIYFPSGVLDPGDVACGRVDFDRNVRREIAEETGLSLEDVTAD
                     PWLLVLAPPRVVLIKVFRFRQSAGEMRASILAHLQKETLSELSDVHIVRDPADFKPSM
                     PPFVSAFLLHFWACDRER"
BASE COUNT          606 a         1016 c         1007 g          606 t
ORIGIN      
        1 cggcttgggg ttctcgttcc aaagcatcgc ctccgcgcgc ttgcggaaca tgccgaggct
       61 cggccagttc gggttcgcgg cgatgaaggc gaggaagcgc ttgctgtcgc cgccgctgtg
      121 atcgctgcgc aaaatggtcc actcgacgag cttgcgcgcc accgggtcgg agatcgcggc
      181 ggcagcttcg gtcgccttgc tggcgccgcc gtggcgcagc gcctcgatgg cgctcttgac
      241 atgcgcaatt tcggcggcgg aggtctccgg cgtcggaccc ggcgccagcg gtatctgcgg
      301 caccgcggtt ttggaggccg acttatcgtc cttggatttg cgcgccgccg ccttctttcc
      361 cccggacttc gcggtcttgc ccgcggcttt cgccgtggtg gcggaggagc ctttgccgcc
      421 gtgtgtggtc ctggccgcgg cgtagtgact agtggcgagc agactcgcgc aagcgagtac
      481 agcaacgagt gctgagctgt gtcgtcgtgt caatttggct gatccccaat cccggcggat
      541 tactgcgctc cccagcgcgg caatcacgcg ccccccacgt gatagcgccc atgctaccca
      601 cgatttgggc ggaaaagcga tgccgggatg gcaattcatg gccccaagcc gatggcacgc
      661 gcgcgatccg cacccgcaca tcgtcgtcta tcgagggctt ggcgttatga tcccgatggc
      721 cagcactgcg cgaacgtcac gggatgtccg agacgtgcga ttgcagcgct tcatgttaag
      781 gtggcaaagc cgaccgcgta tgcggtccgt cgcatcctgg ccggtgatga cggacgcggt
      841 cgacgtaggc ggggaaatcc atgcccgacg tgaggcgacg agagttcatc ctattgccga
      901 cgggctcgcg gccgccgggg cgcgcgcggc gcacgcggca atccccacgc ggctatcgcg
      961 gagcggctgc agccacgcat cttgggcggc tgaccgccgc cctcgcggca acaattctgt
     1021 tcggcagctt gctatcgtcg gcagccgagg cccgtatcct cctatgctgg tgggagtatc
     1081 cgggtcggat gtgcacgatc atcaaatccg ccaaatcgac catgcgaccg cactgggccg
     1141 gctacggtta tcgccgagcg ccgcccccat gcccgcgggg attttgggcg gtctgtggcg
     1201 agtgcccctg ctagaggtcg gattcacggc ggcgatggcc gccagcggag ccgcgtggac
     1261 tggtgcccat gccgggctcg gtcgctgaaa tatcgccgtc tctcgtcgcc ggattcgtgc
     1321 tttagcgcag ttcgagatgt ccctcacctt cgagggtcgc aaggtcgatg cgggcgacgc
     1381 agctgacttg aacataatcg agcgtcaggt ctcccaccac tctgatctcc gcagacccat
     1441 tgtcgctttc gatggcgcgc aagtcggagc ggctgcggtc gatctccacg ccgagttcgg
     1501 tgcctcctcg cgtgtcggtg aatttgatgc gaacatgacc gttcgcgatg gattccttca
     1561 acgcctcgcg cgttttttcg cgcagcctcg cttcgatcgg atgatcgcct ttgcagagaa
     1621 attgtaccaa ctcgctcact ccacccctcc tcgttggttt tgcaatgacg gcaatgttcg
     1681 ttcctgttcg cgccaattgg cgctattcgg gctccgcggt caacccacct tgatcctgcg
     1741 cggtgctcaa gcctcagggt gctgggggat agccgagcgc caccacgatc tgtgaggcga
     1801 gcgccactcc aattcccgcg ctcagcagca gcgccgtccc tgcgaccacg tagtgcagat
     1861 cgctcgggct cttatgccaa taccgtctgc gggtccacgc tcgcaaaatc caagccgtcg
     1921 tccccacgca gaagagaacg gaggcgagat agatcgaaac aaccacgagg gccatgccgc
     1981 gcagaccagg cgaatctgct cagatgttac atcgcagcga aagccgcggc catgccgtcg
     2041 cccgagcaga aaccgacgtc atcgctcgcg gtcgcaagcc caaaaatgca gaagaaacgc
     2101 gctgacgaat ggaggcatgc tcggtttgaa gtcggcgggg tcgcgaacaa tgtgaacgtc
     2161 ggaaagttca gatagggttt ctttttgcag atgagcgaga atgcttgcgc gcatctcgcc
     2221 cgccgactgg cggaagcgga ataccttgat gagcacgact cgcggcggcg cgagcacaag
     2281 gagccaggga tcagctgtaa catcctcaag gctgagaccg gtctcctcgg cgatctcccg
     2341 ccggacgttg cggtcgaaat cgacacggcc gcaggcaaca tctcccggat cgagcacgcc
     2401 ggacggaaag tagatgccgc cggcaccggc ggtatgggca ttcatcacgc ccaacaagaa
     2461 cgcatcatcg cgggatagca acgcccccat cgcgaagcac gcgtggacgt cccggtcggg
     2521 aaagccccag tcgcgccaag cgatgaaact tgcgaaatca gtctcgaaca tagtgccctg
     2581 cagcgtggcg ccggaaatct caaaatcgcg cagcaacagg acacgcccgt tccacaattg
     2641 gggcttttcg cgccgcagat gcgtaaaata gtcttcgatt tcggtgcggc ggtccgcagc
     2701 gaatggccat ggcagaggaa aaacttgaaa atccagacgt tcaacggaaa ggatatcgat
     2761 gtcggtcatt ttgcactctc ggcgctggca ggcgctgcgc aggaattcgc tgccggctgg
     2821 tcaggcccgc tcgaacctcg gcagcaacct tttccgtgta gacgtccttg gacgccgatc
     2881 ctatcgcatt tgaccgaaaa ccgtgatcga tgcgagtttc aagttgcgat gatcaaccca
     2941 tcggccagcg actcgcatag tgcccggctc agtcagtgca gcgacgttcg acacgacgcc
     3001 catgacctcg gcttcgatcc aaggcctgct tgcgagccgc tcgagatcac ggactgcgct
     3061 ggagctgcga cgcaaccagc cgaggcgaga atccggtcga cttgacggag caatcgaatg
     3121 atttggagag tggggaaggt agagaacatc gacatcgggt cgggcttcat tcccagcccc
     3181 gggttcacga tccaacaaga cggccggtct ccctcgctga cgataatctt tgaag
//
LOCUS       DATLJT010000847         5005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3960110,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000847 DATLJT010000000
VERSION     DATLJT010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3960110"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VM910_19315"
     CDS             complement(<1..221)
                     /locus_tag="VM910_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64712.1"
                     /translation="MRAVRVRTLTVALLILSLAPFERAAAQDFPSRPVRFVVPYAAGG
                     SGDLLARLLGNKLASIWGQQVVVDNRPGAG"
     gene            complement(250..933)
                     /locus_tag="VM910_19320"
     CDS             complement(250..933)
                     /locus_tag="VM910_19320"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64713.1"
                     /translation="MSFREVPGPAQSNIEHWSRDNFQQHVLDPVVTAVRYLASRHVDY
                     IIHTGMPVVTTRGKGFEDELVKQIEDATGLPATTSIRSAIRALAHLGVRNVAVVTPYP
                     QELHQSALAFLAASGFRVVSDHTMDVVFKRLQDVTPAQIFATATRVLASASSADGVYI
                     PCNQWSGADAAPLIENELGIPVVTGAHADYWEAFRSLGITDRIAGHGRLMLSLSEMAP
                     APMTAAARG"
     gene            complement(1263..1517)
                     /locus_tag="VM910_19325"
     CDS             complement(1263..1517)
                     /locus_tag="VM910_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64714.1"
                     /translation="MIEKITDLRLERRLRRIRKPDGNCAHCERSADRFHPVGPIVVRI
                     WEPDTEDEPFTYEFCCWECLGHWAAVQADFVAPNYDPPRR"
     gene            complement(1522..1674)
                     /locus_tag="VM910_19330"
     CDS             complement(1522..1674)
                     /locus_tag="VM910_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64715.1"
                     /translation="MRKMEERKDKKPRRPRAKKKPEASQAERDAALDDLIGAAPATRS
                     RSHVME"
     gene            2132..2422
                     /locus_tag="VM910_19335"
     CDS             2132..2422
                     /locus_tag="VM910_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64716.1"
                     /translation="MPDTRRPKRRRLEDLRREWVPPPVDDAAWEDHLRWMALHPIRFS
                     REWLFPGEKRLPTSRQSRLIRQRQNRFLAAHPEYLEAARRKAEEADGAQRGS"
     gene            2802..3047
                     /locus_tag="VM910_19340"
                     /pseudo
     CDS             2802..3047
                     /locus_tag="VM910_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
     gene            3623..4132
                     /locus_tag="VM910_19345"
     CDS             3623..4132
                     /locus_tag="VM910_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64717.1"
                     /translation="MPIKALRANVLCVRHNSAWSQLDAMAGRFFRALAELYDDLGRRT
                     LSRKPIWRLFSGEELELWLLKTILGFFHAGVLSKDGRKIGDVQTVMNLAIEAAYRTGR
                     LIEPCGIYVLKDVTLLAQCGSLDFASLSDERDERHSGLPSYYDGAGHDTLHRSEHAQS
                     TPVHSQPFV"
     gene            4255..4449
                     /locus_tag="VM910_19350"
     CDS             4255..4449
                     /locus_tag="VM910_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64718.1"
                     /translation="MAADTFACISQYVALATGDCDVLADGVVVGRLALGHHEDRTPTH
                     GYAATREAAMAAFAKSWRRE"
     gene            4456..4752
                     /locus_tag="VM910_19355"
     CDS             4456..4752
                     /locus_tag="VM910_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64719.1"
                     /translation="MHDEQIARWNQQRGPRVGDWCETKDDSLHRIGGVRGGRIPLASP
                     SGRFSLGSEGVTYFGIFIDHPAIPTSSLLKQPERKPGAIHQPHFTVDCRVFKER"
     gene            4832..>5005
                     /locus_tag="VM910_19360"
     CDS             4832..>5005
                     /locus_tag="VM910_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HVI64720.1"
                     /translation="MPDQATEASQPTSSHSLPALSLAALGVVFGDIGTSPLYALKAVL
                     DVTTENPPTEILGA"
BASE COUNT          975 a         1564 c         1546 g          920 t
ORIGIN      
        1 cccgcgcccg gccgattgtc gaccacgacc tgttgtcccc agatgctggc gagcttgttg
       61 ccgagaagac gcgccagcag gtcgccgctg cccccggctg catagggcac gacgaagcgg
      121 accggccgtg agggaaaatc ctgcgctgcc gcgcgctcga acggcgcaag cgacaagatc
      181 agcagcgcaa cggtcagcgt acgaacgcgg acggcgcgca ttgctctgcc tccctgccgg
      241 aaaatgcgtt catccacgcg ccgcggccgt catgggcgcc ggcgccatct cgctcagact
      301 cagcatcagc cggccgtgac ctgcgatgcg gtcagtgatg ccgagggagc gaaacgcctc
      361 ccaatagtcg gcgtgcgcgc ccgtcaccac cgggatgccg agttcgttct cgatcagcgg
      421 cgcggcgtcc gcccccgacc actgattgca ggggatatag acgccatccg ccgacgatgc
      481 tgaagccaaa acgcgcgtgg cggtcgcgaa aatctgcgcg ggcgtgacgt cctgcagccg
      541 tttgaagaca acgtccatgg tgtggtcgga gacgacgcgg aatccgctcg cggcgaggaa
      601 agcgagcgcg gattggtgca actcctgtgg atagggcgtc accaccgcga cattgcgaac
      661 cccgagatgc gccagtgcgc ggatcgcgga tcggatgctg gtggtggcgg gcagtcccgt
      721 cgcgtcctcg atctgcttca cgagctcatc ctcgaaccct ttgccgcgcg tggtcaccac
      781 cggcattccg gtatggataa tgtagtccac gtggcgcgac gcgagatatc gaaccgcggt
      841 cacgaccgga tcgagcacgt gctgctggaa attatcgcgg ctccaatgct cgatgttgga
      901 ctgggcaggt ccggggacct cacgaaagct caatatcgac gagccaattt aggaagggcg
      961 cctgcccagg aattggcgct gggcaggtcc cgggcaggtc cggccgatct aacgcaccgg
     1021 cacccgctcc ccgtggcggc gcgaggcctt ctaaacgcga tcgagggtcg aaaatgagag
     1081 cggcccgacg cgagtggtta gtcctcgccg ggccgtctca agggcgggcg cttgcgcgcc
     1141 gacccgccag tcatcaggag atgaacagct gaattataac atcaatccac ggatgagaga
     1201 atgagcgtca gcgacggagc ggcgaagaac agcaggaggc atggcgtctc gcgcgccgcc
     1261 cgctatcgtc gaggcggatc atagttcggg gcgacgaagt cggcctgcac ggcagcccaa
     1321 tggccgagac attcccaaca gcagaattcg tatgtaaaag gttcatcctc ggtgtcgggt
     1381 tcccagatcc tgacgacgat cgggccgacc ggatgaaagc gatcggcgga ccgctcacag
     1441 tgcgcgcagt tgccgtccgg cttgcggatg cgccgtaggc gtcgttcgag ccgcagatct
     1501 gtgatcttct cgatcatagc ttcactccat gacgtgtgat cttgagcgtg ttgccggcgc
     1561 tgctccgatc agatcatcga gcgcggcatc gcgctccgcc tgagacgcct ccggcttttt
     1621 cttggcgcgc ggccgcctcg gctttttatc cttgcgctct tccatcttgc gcacgtactc
     1681 agtccactgc tgacgttctc gatttcgcat agcgctgtta tatactcggt catgtttccg
     1741 ttccccgatc ccgcgcacgt tggatacaat ccgtatttgc gggcagactg gaccgagctc
     1801 cagttgcgat tccgcccacc gcgggacagc cctgacgtca gtatccgtct gctcgactat
     1861 gccagccccg aagagatcaa cagcgtcatc gcgcgctata gctgcactgg cgcgagctca
     1921 gcgcacaggc gcgcagcgct aatgggacgc ctacccgccg catgtccgcc ggggcgcagg
     1981 acgctgccct ggccaccatg ggggccgcca cggaccggcg ccggattggc tggatcctcg
     2041 gacgtctgcg cgaggacgtc gtccacaccg actaccgcga tcatgccgcg ctgctgccgg
     2101 aggggccagc aaaggatctg ctgccagttc tatgcccgat acgaggcggc ccaagcggcg
     2161 gcggctcgag gatctacgcc gagagtgggt gccgcctccc gtcgatgacg ccgcctggga
     2221 ggatcacctg cgctggatgg cgctgcaccc catcaggttc tcgcgcgaat ggctgtttcc
     2281 cggcgagaaa cggttgccga cctcgaggca gtcccggctg atccggcagc ggcagaaccg
     2341 gttcctcgcc gcccacccgg aatatctgga ggcggcccgg cgcaaggcgg aggaggcgga
     2401 tggcgcgcag cgcggcagct aggaagcggc gcgtcgccga cacccaggcc tggcgcgccc
     2461 ggcagaagcg cggcgccgcg gtctatccgg tcgaggtcga cggcgagctc ttcaatctga
     2521 tggaacgttt cggcggctgc agtagcgccc tgaactccta ctttggcaga tttctcaaca
     2581 cgagcggggt gatagaacct gtgggctcga cacacgagcg cgcccccgga acgggtagag
     2641 gagttgtttc gatggtgatc gggacgagcg ccattgtctt cgccgccgca gctgctgtcg
     2701 gagtagccgc ttgcgcgcga ccggctgatg cgcgttttat gcaccagtgc agcggcaaat
     2761 atcacgctgc ggcaaaagct ggatgcgggg tcaatgcagc gaaggcccgt gcaccactgc
     2821 cgcgggggat cacgcttggc atcggtgacg tagtaagcgt cacgattttt gagccccctg
     2881 gtgggctgtt cgtcccaagc gacgccggcg cgcgacccgg caacttcgtg acgctgccga
     2941 accaaatagt ggactccaac ggcaacataa cagtgccgta cgccggcacg atcccagccg
     3001 ccgggaggac accttccgaa gttcaacaag cgatcaacga agctctaagg gccttgcctc
     3061 ggtatcggag cgggaaatag atgcctctca ccctccgccc gacgggccgc gcatcgccgg
     3121 cgcaagccga tcgccaagaa cggtctacga agaaggccag ccgaccggcc gcatctacga
     3181 ggatggctcg gccagcacgc cgtgcgctgg ttctggtcga agcagcacag accccgtagg
     3241 gcggccggat catcaacaac gagaaggggg caaggtccgc aaaactgcca agaattaaat
     3301 gtctcgacgt cgcgatcctg ccgttaccaa aattgttcat ctgccgtggc gatcgacaga
     3361 cgtttgccca tgcggcagtg gcgatacata cggccgctgt tgtcgccaac tcagcggatc
     3421 gccctacaag acgagctgca actataaacc atccggagct tcgacggcct attcgcatcc
     3481 gagctgctat atgaattgga cacgcaactg ctcacgggcg atctcagggg aacatctcat
     3541 ctccgagacg gtcctatcaa ttctgaaccc tacagcactg cggatcgctg ggttggaatg
     3601 gcaaaaagga gagacccgag acttgccgat taaggcgctt agagcaaacg ttctttgcgt
     3661 tcgccacaac agcgcttggt cgcaacttga cgcgatggcg ggaaggttct tccgcgcgct
     3721 cgctgagcta tatgatgatc tgggccggcg cacgttgtcc agaaagccaa tttggcgcct
     3781 cttcagcggt gaagaattag aattgtggct cctaaaaaca attctcggct tcttccacgc
     3841 cggcgtgctg tccaaggacg gaagaaaaat tggagatgtc cagacagtca tgaatctcgc
     3901 aatcgaggca gcctatcgca ccggccgtct tatagagccg tgtggcattt acgtgctcaa
     3961 agacgtgacg ttattggctc agtgcggcag ccttgacttc gcgtctctgt cggatgagcg
     4021 cgacgagcgg catagtgggt tgccgtctta ctatgatggg gctggtcatg acactcttca
     4081 ccgatccgaa catgctcaat cgacacctgt tcacagccaa ccattcgtat agaccagact
     4141 accttttttt ccgcaacagt cgccgccgtc actcggttgt tctgacctgg cctacacgcc
     4201 gacctcgacg cgctgtgatg ttcgaaatga accgagccgc tacttgaatt taggatggcg
     4261 gccgacacgt tcgcctgcat cagccaatac gtggctctgg ccaccggcga ctgcgacgtg
     4321 cttgccgacg gtgtcgtggt cgggcgcctc gcgcttgggc atcatgagga tcgcacgccg
     4381 acccacggct acgcggcgac gcgcgaggcc gcgatggcgg cattcgcgaa gagctggcgg
     4441 cgggagtaac ggcctgtgca cgacgagcag atcgccaggt ggaatcagca gcgtggaccg
     4501 cgcgtggggg actggtgcga gacgaaagat gacagcctgc accgcatagg cggtgtcagg
     4561 ggcggaagaa ttccgctcgc cagtccgtcc gggcgcttct ctttaggctc tgaaggggtg
     4621 acttattttg gcatcttcat tgatcatccg gctattccaa cgtcgtcgct gctgaaacag
     4681 ccggagcgca agcccggtgc aatccaccaa ccacatttca ccgtggattg tcgcgttttc
     4741 aaagaacgtt gataatcccg ttctccaata gctaaccgca ccagctctca cgacttgagc
     4801 ccgtagtaca tgtacttatg atctttctac catgcctgac caagcgaccg aggcgtctca
     4861 gccaacttct tcccacagcc tgccggcgct gagcttagcc gcccttggcg tggtgtttgg
     4921 cgacatcggc accagtccgc tctatgccct gaaggccgtt cttgacgtca caactgagaa
     4981 tccgccgacg gaaatcctag gcgcg
//
LOCUS       DATLJT010000848         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2582867,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000848 DATLJT010000000
VERSION     DATLJT010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582867"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VM910_19365"
     CDS             <1..451
                     /locus_tag="VM910_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HVI64721.1"
                     /translation="ESYGKAIFKALRKHFGNNLPNTFVEPGRGLVGNAGIIEAEVVLI
                     AKRSPEDEVRWVYLDIGKFHGLAETIGESIRYPIRTTHDRDETAPCIIAGPTCDSVDV
                     LYEKTPYPLPVSLAIGDKVLIEAAGAYTATYSSVGFNGYPPLRQYVI"
     gene            complement(774..1778)
                     /locus_tag="VM910_19370"
     CDS             complement(774..1778)
                     /locus_tag="VM910_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HVI64722.1"
                     /translation="MAPSGSDTVSVVERTRPIAIGTGIVGAHFLGRTAVFVLGEEAIV
                     LAPPDGEPRRIAAHDGAILATAAGDSRVVSAGDDGKIVATDADGASGVLARDPKQRWI
                     DHVALGPDGVVAWSAGKTAFVQAKELREFEAPSTVGGLAFFPKGLRLAIAHYNGATLW
                     FPNAAGAAPEQLEWKGSHLGATVSPDGRFLVTTMQEPMLHGWRLADRQHMRMSGYAAR
                     VMSLSWSAGGHWLATAGASQLVLWPFQGKDGPMGKTPRLLTPSGHRVDIVACHPGTDV
                     VAAGYNNGLLLLARIEDGAEILAKKPGDAPVTALAWSGDGRLLAWGTENGEAGIVNVG
                     "
     gene            complement(1786..>2416)
                     /locus_tag="VM910_19375"
     CDS             complement(1786..>2416)
                     /locus_tag="VM910_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVI64723.1"
                     /translation="KNQIAFADVILINKTDLVSPDELSDVEARIRGINPYAKLHKTQR
                     AKVPLDAVLGRNAFDLDRILDLEPDFLGDEHDHHHHHDHDHGHHHGHDHDPTHSHSHG
                     GLKHYHDEDMQSIALHTDKPLDPDKFFPWVQDLVQKEGPSILRCKGILAFKDDDQRFV
                     FQGVHMILDGDHQRPWKDGEKRDSRIVFIGRNLPGKMITEGFESCIAKA"
BASE COUNT          432 a          763 c          759 g          462 t
ORIGIN      
        1 cgaatcctac ggtaaggcga tcttcaaggc gctgcgcaag cacttcggca acaatctgcc
       61 gaacacattc gtcgagccgg gccgcggcct ggtcggcaat gccggcatca tcgaggccga
      121 agtggtgctg atcgccaagc gtagtccgga agacgaggtc cgctgggtct atctcgacat
      181 cggcaagttc cacggcctcg ccgagaccat cggcgagtcg atccgctacc cgatccgcac
      241 aacgcatgat cgcgacgaga ccgcgccgtg catcattgcc ggcccgacct gcgactcggt
      301 cgacgtgctc tacgagaaga cgccgtatcc gctgcctgtc tcgctcgcga tcggcgacaa
      361 agtcttgatc gaggcggccg gcgcctatac cgcgacctat tcctcggtcg gcttcaacgg
      421 ctatccgccg ctgcggcagt acgtgatctg atatcgcgcg ctcggggttc tcatttcggt
      481 tgaaccttgg gatcgcagcc cggactgccg ggcgacggga tcaccggcat tttaccggtc
      541 tccggtgccg gcgttttcat gcctggatcg acatccggtg gacagatcac gccgccgccc
      601 cgatcgagct tgtcgctgag cgacttcgat gaatcgccag gttcggcgca gcgtttttga
      661 tcggcgcttg cgcgcaagca agcgtaagcg ccagcacgct tccggagcgg gcactgatcg
      721 gtttcacccc cggataatga atggggcgat gcgccagtcc agctttccca cgctcagccg
      781 acattgacga tgccggcttc gccgttttcg gtgccccagg cgagcaggcg gccgtcacca
      841 ctccaggcca gcgctgtgac cggtgcgtcg cccggcttct tggccaagat ctcggcaccg
      901 tcctcgatgc gggccaacag gaggagaccg ttgttgtagc ccgcggcaac gacatcggtg
      961 ccgggatggc aggcgacaat gtcgacgcgg tgccccgatg gcgtcagcag ccggggcgtc
     1021 ttgcccatcg ggccatcctt gccctggaac ggccacagca caagctggct cgcgccggcg
     1081 gtagcaagcc aatgaccgcc cgcgctccag gacagcgaca tcacccgcgc cgcgtagccc
     1141 gacatccgca tgtgctggcg atcggcgagg cgccagccat gcagcatcgg ctcctgcatg
     1201 gtggtcacca ggaagcggcc gtcggggctc accgtcgcgc caagatgcga gcccttccat
     1261 tcgagctgtt ccggcgccgc gccggcggca ttgggaaacc agagcgtggc gccattgtaa
     1321 tgggcgatcg caagtcggag acctttgggg aaaaacgcca gcccgccgac ggtcgatggc
     1381 gcctcgaatt cacgcaattc cttcgcttgc acaaaggcgg tcttgccagc ggaccaggcg
     1441 acgacgccat ccggtccgag cgcgacatgg tcgatccagc gctgtttcgg gtcgcgggcc
     1501 aagacgccgc ttgcgccatc ggcgtcggtg gcgacgattt tgccatcatc gccggcgctg
     1561 acgacgcggc tatcgcccgc tgccgtggcg aggatagccc catcatgggc cgcgatccgg
     1621 cgcggctcgc catccggcgg cgccagcaca atggcttctt cgccgagaac gaatacggct
     1681 gtgcggccga gaaaatgcgc gccgacaatg ccggtgccta tcgcgatcgg ccgggtgcgc
     1741 tcgacgaccg aaactgtgtc gctgccactc ggggccatct tagctctacg ctttggcgat
     1801 gcagctctcg aagccctcgg tgatcatctt gcccggcaga ttgcggccga tgaaaacaat
     1861 ccggctgtcg cgcttctcgc cgtctttcca tggccgctga tgatcgccat cgagaatcat
     1921 gtgcacgccc tggaagacaa aacgctggtc gtcgtccttg aaggctagga tgcctttgca
     1981 gcgaagaatg ctggggcctt ccttctgcac caggtcttgc acccacggaa agaatttgtc
     2041 gggatcgagt ggcttgtcgg tgtgcagtgc aatcgattgc atgtcctcgt cgtgataatg
     2101 tttgagcccg ccatgtgaat gtgaatgcgt aggatcatga tcgtgcccgt gatgatggcc
     2161 gtggtcatga tcatgatgat ggtgatggtc gtgctcatcg ccgaggaagt caggctcgag
     2221 atcgaggatg cgatcgagat cgaaggcatt gcggcccagc accgcatcca atggaacctt
     2281 ggcacgctgc gtcttatgca acttggcgta aggattgatg ccgcgaatgc gcgcctcgac
     2341 atcgctgagt tcgtcgggcg agacgagatc ggttttgttg atcagaatca cgtccgcgaa
     2401 agcgatctga ttcttt
//
LOCUS       DATLJT010000849         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4190638,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000849 DATLJT010000000
VERSION     DATLJT010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4190638"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VM910_19380"
     CDS             complement(<1..815)
                     /locus_tag="VM910_19380"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028174674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVI64724.1"
                     /translation="MSAKNGAIKLVAGNSSPVLAKSIADGLELPLTKTVVRRFADMEI
                     FVEIQENVRGSDAFVLQSTSYPANDHLMELLIITDALRRSSARRITAVIPYFGYARQD
                     RKSGSRTPISAKLVANLITNAGVDRVMTLDLHAGQIQGFFDIPTDNLYAAPLMVRDIK
                     DRFDLTNVMVVSPDVGGVARARGLAKRINTPLAIVDKRRERPGESEVMNVIGEVEGYT
                     CILVDDIVDSGGTLVNAADALIAHGAKEVYAYITHGVLSGGAVTRITSSRLKE"
     gene            complement(979..1587)
                     /locus_tag="VM910_19385"
     CDS             complement(979..1587)
                     /locus_tag="VM910_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64725.1"
                     /translation="MRDASINLPRLLPAAIIVLRAGVVALLLAAACALGGCASSNSNA
                     FAMASDGPTVAFESIDGPPPQVFDRMVSVLDTESKLRSLSIVSREGAAAYRVRFYLAA
                     QVNRGRTVVAWVWDVYDRDQQRALRLSGEELAGKAGRDAWAAADDLVLRKIAQAGLSG
                     LSGMINGTGNPQSPPRGPMVASANVPVADNTSGVGALGYTDH"
     gene            complement(1699..>2313)
                     /gene="pgeF"
                     /locus_tag="VM910_19390"
     CDS             complement(1699..>2313)
                     /gene="pgeF"
                     /locus_tag="VM910_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HVI64726.1"
                     /translation="LMAARLGVGPEYLISAHQTHSPDAIVATGPWQNGTRPKADAIVT
                     ASAGLAIGITAADCGPVLLVDPTARVIGAAHAGWKGALTGILESTIEAMEKLGAERSG
                     MVAAIGPLIRQQSYEVGAEFIERFLDADADNAAYFIPAGRDGHAMFDLAGYIRMRLEN
                     AGVLMIDDLGVDTYSDERFYSYRRSVHRKEADYGRHVHAIALEG"
BASE COUNT          411 a          718 c          778 g          406 t
ORIGIN      
        1 aattccttca gccgcgagga ggtgatgcgg gtcaccgcgc cgccggacag aaccccgtgg
       61 gtgatgtagg cgtagacctc cttggcgccg tgcgcgatca gcgcatcggc cgcgttcacc
      121 agcgtgccgc cggagtccac gatgtcgtcg accaggatgc aggtgtagcc ctcgacctcg
      181 ccgatcacgt tcatgacctc cgactcgccc ggccgttcgc ggcgcttgtc gacgatcgca
      241 agcggcgtgt tgatgcgctt ggcgagcccg cgggcgcgcg ccacgccgcc gacgtcgggc
      301 gacaccacca tcacattggt gaggtcgaag cggtccttga tgtcgcgtac catcaacggc
      361 gcggcgtaga gattgtcggt cggaatgtcg aaaaagccct ggatctggcc ggcatgcagg
      421 tcgagcgtca tcacacgatc gacaccggcg ttggtgatca ggttcgccac cagctttgcc
      481 gaaatcggcg tccgtgagcc ggatttccga tcctgccgtg catagccgaa atagggaatg
      541 accgcggtga tgcggcgcgc ggacgatcgg cgcagcgcat ccgtgatgat cagaagctcc
      601 atcaggtggt cgttggccgg atacgaggtc gactgcaaaa cgaaggcgtc ggagccgcga
      661 acgttctcct ggatttcgac aaaaatctcc atgtcggcga aacgccggac cactgtcttg
      721 gtcagcggca gttcgagccc gtcggcgatg gatttggcca gcacggggct ggaattgccc
      781 gcaacgagct tgatggcgcc gttcttggcc gacatcgagg cttctccgcg ctccctgctg
      841 gaggccttgt tcagcatacg gggtttccct gaaaccggct aattttgacg agcgagggaa
      901 tatcaggggg gacgcgcggc tggcaacccg cgaatgggga tttcttgggc aaaaacagag
      961 cttcctgccg ggttttggct aatggtcggt gtagccaagg gcgccgacgc cggaggtatt
     1021 atcggcgacg ggcacgtttg cacttgcgac cataggcccg cgcggggggg attgcgggtt
     1081 acccgtgccg ttaatcatgc cggagagacc ggacaggcct gcttgcgcga tcttgcgcag
     1141 cacgaggtca tcggcagcgg cccaggcgtc gcggccggct ttcccggcca gttcctcgcc
     1201 ggacaggcgc agcgcgcgct gctggtcgcg gtcatagacg tcccagaccc aggcaaccac
     1261 ggtccggcca cgattgacct gtgccgcgag atagaagcgg acgcggtagg ccgccgcgcc
     1321 ctcccgcgaa acgatcgaaa ggctgcgcag cttgctctcg gtgtcgagca cgctgaccat
     1381 gcggtcgaac acctggggcg gcgggccgtc gatcgactcg aatgcgacgg tcggcccatc
     1441 gcttgccatc gcaaaagcat tggaattgga agacgcgcag ccgccgagcg cgcaggccgc
     1501 cgcaagcagc agcgccacga cgccggcgcg caacacgatg attgccgccg gcaacagccg
     1561 tggcaggttg attgacgcgt ccctcatttg gctttcgcgt tatcgctaaa ttgcgggagc
     1621 gtctagagca gcggcgacac aggttggaga cgaaatgaag ggcgaattgc ccagaatttg
     1681 ccttgagtct gggacagctc agccttccag cgcaatcgcg tggacatgcc ggccgtagtc
     1741 ggcctccttg cggtgcaccg agcgacggta gctgtagaag cgctcgtcgg aataggtgtc
     1801 gacgccgaga tcgtcgatca tcagcacgcc ggcattttcc agccgcatgc ggatgtaacc
     1861 ggcgagatcg aacatggcgt ggccgtcgcg gccggcgggg atgaaatagg cggcattgtc
     1921 ggcatcggca tcgaggaagc gttcgatgaa ttcggcgccg acttcataac tctgctggcg
     1981 gatcaggggg ccgattgcag cgaccatgcc gctgcgctcc gcgccgagtt tttccatcgc
     2041 ctcgatggtc gattccagga tgccggtcag cgcgcctttc cagccggcat gcgccgcgcc
     2101 gatcacgcgg gcggtgggat cgaccagaag caccggcccg cagtcggccg cggtgatgcc
     2161 gatcgcaagc cccgcgctcg ccgtgacaat cgcgtccgcc ttcggccgcg tcccattctg
     2221 ccaggggccg gtcgcgacga tcgcgtcagg cgaatgggtt tggtgcgcgc tgatcaggta
     2281 ttccggcccg acgcccaatc gcgccgccat gag
//
LOCUS       DATLJT010000850         6214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1433556,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000850 DATLJT010000000
VERSION     DATLJT010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433556"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1113
                     /locus_tag="VM910_19395"
     CDS             130..1113
                     /locus_tag="VM910_19395"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64727.1"
                     /translation="MRRRDFITLLGGAAAAWPLAARTQPAERMRRIGVLMGVADDAEG
                     QARVKGLQQGLQELGWIEGGNVRIDYRWTGGDVSRVRAYVTELVKLSPDVIVAHTPPV
                     IAAMRQASGSIPIVFAAVIDPVSHGFIDNLARPGGNLTGTTNIEFSLVGKMLEMLKEV
                     APGIMRAAVIFGTNSQYLVYLHSFGTTPPTLAVELSAAAVHDTGEIEAAISELGRKPG
                     AGLVVPPDAFTVIHHAMIMELAARHRLPAVYAYRSFVAEGGLMSYGPDVHDIFRRTAS
                     YVDRILKGAKPADLPVEAPVKFEFVINLKTAKALGLDVPDKLVALADEVIE"
     gene            1332..1724
                     /locus_tag="VM910_19400"
     CDS             1332..1724
                     /locus_tag="VM910_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64728.1"
                     /translation="MTAFNPFAAAAIVALCAVGSPHMAQAAEPETLTLACQGTVTNNI
                     KPDAKPEQITMGIVVNFTAQTVAGFTYPGLEEFPVVIRAASEVLIARLTVLRCTLGGS
                     LCGFTFGPSGRLRLPRAAAKDSGAHELT"
     gene            1784..2020
                     /locus_tag="VM910_19405"
     CDS             1784..2020
                     /locus_tag="VM910_19405"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI64729.1"
                     /translation="MLKDASRRQFAIDTATPMGKLVFQLTGALAEFERTMIRQRIKAG
                     LKRAVAQGVKLGRPKIDSATERRCETSLRKAWVF"
     gene            2140..2931
                     /locus_tag="VM910_19410"
                     /pseudo
     CDS             2140..2931
                     /locus_tag="VM910_19410"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2928..3908
                     /locus_tag="VM910_19415"
     CDS             2928..3908
                     /locus_tag="VM910_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64730.1"
                     /translation="MSSRRDFIALLGGAAAWPLAARAQQAERVRRIGVLMGAVESDPA
                     RRSQLAAFRGALAKLGWSNLGIEVRWGADPELCARYAAELVALGPELLLADSSPSLEA
                     LQRQTRTIPIVFVTVADPVGQGFVANLAHPGGNITGFSVFDAPMASKWLEMLTQITPP
                     VTRVAMLFNPATTPYANLILQAMEEAAPSFAVAVRAAPVNSDFEVDAMMAALAREGRG
                     GVLVLTSIFTATNRDAIITLAARHRLPAVYAFPFFAEAGGLMSYGVDVADVHRRAADY
                     VNRILNGDKPGDLPVQTPTKFHLVINLKTARALDITVATSLLATADEVIE"
     gene            3905..4882
                     /locus_tag="VM910_19420"
     CDS             3905..4882
                     /locus_tag="VM910_19420"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64731.1"
                     /translation="MSNRREFITLLGGAAAWPLAARAQQPATPVIGFLGAVPPSNVPK
                     WVDGFRQGLAETGYDEGRNVAVEYRWSDSDYDRLPALAADLIERRVAVIVAAGVDPAH
                     AANTATTTIPIVFFMSGDPVAENLVSSLNQPSGNLTGVTTFSGVLTSKRLELLRELAP
                     SARIAALMNPNNSNAKFRLKEIEKASRILDLRVEIVNTRNADEIDGAFASLLQRGAGA
                     LLVVDDPLCRGARSKLVALAMRNRIPTMYFQSEFVQVGGLISYGSNYAERFRQVGIYT
                     AQILKGAKPAELPVVQPTRFELVINLRTARALGLDVPPTLLARADEVIE"
     gene            4879..5871
                     /locus_tag="VM910_19425"
     CDS             4879..5871
                     /locus_tag="VM910_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64732.1"
                     /translation="MIGRRQFITLLGGAAVVGPLAARAQQAERVRRIGVLMGAVESDP
                     ARQSQLAAFRGALAKLGWREGSNLGIEVRWGGDPELCARYAAELVALGPEVILADASP
                     SVEALQRQTRTIPIVFVTVADPIGQGFVANLAHPGGNTTGFSVFDAPMAGKWLEMLTQ
                     ITPPVTRVAMLFNPATTPYANLMFRAMEDAAPSFAVAVRAAPVNSESEVDAMMAALAR
                     EGRGGVLVLTSIFTVTHRDAIIALAARHRLPAVYAYSFFAEAGGLMSYGVDVADVHRR
                     AADYVNRILKGDKPGDLPVQMPIKFNLVINLKTAKALDITVATSLLATADEVIE"
     gene            5996..>6214
                     /locus_tag="VM910_19430"
     CDS             5996..>6214
                     /locus_tag="VM910_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64733.1"
                     /translation="MQFGQFGRREFIKLVGGAVVAGPGAALAQTPGRLWKIGVLANES
                     WPPLEGLRDGLRDLGYVEGKTHLFVYRFA"
BASE COUNT         1127 a         1914 c         1988 g         1185 t
ORIGIN      
        1 gaagccggcg gacctgccgg tggtgcaggc ggcaaagttc gagttggtca tcaatgcatc
       61 aacggccaag atgctcggcc tgaccctgcc gcccacactc ctcgcctccg ccgacgaggt
      121 gatcgagtga tgaggcgccg cgacttcatc acgctgctcg gcggcgcggc ggctgcgtgg
      181 ccgctggcgg cgcggacgca gccggcggag cggatgcggc gcatcggggt gcttatgggt
      241 gtcgccgatg acgcagaagg acaggctcgg gtcaaagggt tgcaacaagg gcttcaggaa
      301 ctggggtgga tagaaggggg caatgtccgc atcgattatc gctggaccgg cggtgacgtg
      361 tctcgcgtac gagcttacgt gacagagctg gtaaaactgt caccagacgt gattgtcgcc
      421 cacacgccgc ccgtcatagc cgcgatgcgg caagcatcag gctccattcc gatcgttttt
      481 gcagcagtta tcgatcccgt ctcccacggc ttcatcgata acttggcgcg tccgggcgga
      541 aatcttaccg gtaccaccaa tattgaattc tcactggttg gtaaaatgct ggagatgctc
      601 aaagaagtgg cgcccggtat tatgagggct gccgttatat tcgggacgaa ctcccaatac
      661 cttgtctacc tgcattcgtt tgggaccact ccaccaacgc ttgccgtcga gctgagcgca
      721 gcagctgttc acgacacggg tgaaatcgag gcggcgatct ccgaactggg acgcaaaccg
      781 ggcgctggtc tggttgttcc tccggatgca ttcacagtaa ttcatcacgc gatgatcatg
      841 gaattggcgg cgcggcaccg gctgcctgcg gtctacgcgt accggtcatt cgtcgcggaa
      901 ggcggtctga tgtcgtacgg gcctgacgta cacgacatct tccggcggac cgcttcgtat
      961 gtcgatcgta tcctgaaggg cgccaagccc gcagatttgc cagtggaagc gccggtcaag
     1021 ttcgagttcg tcatcaatct aaagactgcc aaagcacttg gcctcgacgt gccggataaa
     1081 ttggtggcgc tcgccgacga ggtgatcgaa tgagtcgtcg cgaattcatg acgctgctcg
     1141 gcggcgcggc agccgtgtgg ccgctcgcgg cgcgtgcgca gcagccgaag tcgccgctga
     1201 cttgataaaa atggacaagg actatctggt cctcaaacgc gcctcgacct cgcgcccctc
     1261 cggcgagcgg cggggatgag gcgttcgagg ccgcgcaggt gtcgcggatg cttgatggag
     1321 gagggcgcgc catgactgcg ttcaacccct ttgcggccgc tgcgatagtt gctttgtgtg
     1381 cagtcggctc gcctcatatg gcgcaagcgg ccgaaccgga gacactgacg ctggcgtgtc
     1441 agggcacggt gacgaacaac ataaagcccg atgccaagcc ggagcagatc acgatgggca
     1501 tcgtcgtcaa cttcactgcc caaacagttg cgggcttcac ctatccgggt ctcgaggagt
     1561 ttccggtcgt gatacgcgcc gccagcgagg tgctcatcgc tcgactgacc gtcctgcgct
     1621 gcacacttgg cggcagcctc tgcggcttca cgttcgggcc tagcggccgc ttgcgcctcc
     1681 cgcgcgccgc ggcaaaggac tccggtgctc acgagctgac gtgaacgcag tagctgcccg
     1741 gagccgtggg gctctcccgc gaagatgacg cagcctcgat tccatgctca aggacgccag
     1801 tcggcggcaa ttcgccatcg acacggcgac cccgatgggc aagctcgttt ttcagctaac
     1861 cggcgccttg gctgaatttg agcggacgat gatccggcaa cgaatcaaag caggtctcaa
     1921 gcgcgcggtt gcgcagggcg tgaagctcgg ccgaccaaag atagacagcg cgaccgaacg
     1981 gaggtgcgaa accagcttgc gaaaggcgtg ggtattttga aggtggcgag gtccgttggg
     2041 atcgggaccg gcacgccgac gatgcggagg ggcacgcccg gctgacggct ttcgcacagg
     2101 ccctgcagca atcgggttgg accggtcggg caaaatctcc gtatcgacta tcgctggggc
     2161 cccggcaatg cggaaaccat gcgcaaatac gcaaccgaac tggtcgcgct cgggccggac
     2221 gtcatcttgg cgtctactac cgccgccctc gcgccgttgt tggaggcgag ccgcaccgtc
     2281 ccgatcgtgt tcgcgagtgt cgccgacccg gttgccgctg gctttgtcga gagcctggcg
     2341 cggccacgcg gcaacgccac cggctttacc gtctacgaat attccatcag cgggaagtgg
     2401 ctggagctgc tcaaggaaat cgcgcctcgc gtgacgcgag cggcagtcct tcgtgagctt
     2461 ggcatcgcga ccggtctcgc ccagttcggc gcgatccagg cgctcgcgcc gtcgcttggc
     2521 gtggagctgc gccccatcga cgtgcgcgat gctggcgaga tcgagcgcgg ccttgcggcg
     2581 ttcgcgcagg gttccgacgg cggcatcatc gtgatcggca cccccggggc ggctatccag
     2641 cgcgatttga tcatcgcgct ggcggccaag catcgtcgct tacccgcggt ctacaacgcc
     2701 cgcttttacc ccgccgtcgg cggtctcatc tcctatgggc ccgatctcct cgatcagtac
     2761 cggcgcgcgg caggctacgt cgaccgcatc cttaaaggtg agaagccggc cgatctgccg
     2821 gtgcaggcag cgagtaagta tgagctggtc gtcaacctca agactgcgaa agcgctcggc
     2881 ctcgaaatgc cggctagcgt gctcgcccgc gccgacgagg tgatcgaatg agcagccgtc
     2941 gcgacttcat cgcgctgctc ggcggcgcgg ctgcgtggcc gctcgcggcg cgggcgcaac
     3001 aggcggagcg ggtgcggcgg atcggtgtgc tgatgggcgc tgtggaaagc gatccggcta
     3061 ggcggtctca gctcgcggct ttccgtggtg cgcttgcgaa gctgggatgg agcaatctcg
     3121 ggatcgaagt tcgctggggc gccgatccgg agctctgcgc gcgctacgcc gcggagttgg
     3181 tcgcgctcgg ccctgagctg ctcttggccg actcctctcc gtctttggag gcgctgcagc
     3241 ggcaaacacg cacgatcccg atagtattcg tcactgtcgc cgatccggtc ggccaaggct
     3301 ttgtcgccaa tctggcgcac cccggtggta acatcaccgg cttcagcgtt ttcgacgcgc
     3361 cgatggcgag caaatggctt gagatgctca cacaaatcac tccacctgtt acacgcgttg
     3421 cgatgttgtt caatcccgcg acgacgccct atgccaacct aatacttcag gccatggagg
     3481 aagcagcgcc atctttcgct gtagcggtgc gggccgcacc cgttaacagt gatttcgaag
     3541 tcgatgccat gatggccgcg ctggcgcgcg aagggcgcgg cggcgtgctg gttttgacga
     3601 gtattttcac tgcgacgaat cgtgatgcca ttatcaccct ggccgcccga caccgcctac
     3661 ccgcagtcta tgctttcccc ttcttcgccg aggctggcgg gctgatgtct tacggcgtcg
     3721 acgtcgccga tgtgcaccgc cgcgctgccg attacgtcaa tcgcatcctt aatggcgaca
     3781 agccgggcga cttgccggtt cagacgccga ccaaattcca cctcgtgatc aacctgaaga
     3841 cggcaagggc tctcgacatc acggtcgcga catcactcct cgccaccgcc gacgaggtga
     3901 tcgaatgagc aatcgtcgcg agttcatcac gctgctcggc ggcgcggcgg cttggccgct
     3961 agcggcccgc gcacagcagc cggcgacgcc ggtaatcgga ttcctcggcg ccgttccgcc
     4021 cagcaatgtg ccgaagtggg tggacgggtt ccggcagggc ctcgccgaaa ccgggtatga
     4081 cgaggggcgc aatgtagccg tcgaataccg ctggtcggac agtgactatg atcgactgcc
     4141 cgcgctcgcc gccgatttga ttgagcgcag agtcgcagtg atcgtagcgg ccggggttga
     4201 cccggcacac gcggcgaaca cagcgaccac tacgattcca attgtcttct tcatgagcgg
     4261 cgatccagtc gcagagaacc tcgtcagcag tctcaaccag ccgagcggta atctgaccgg
     4321 ggtcaccacg ttctcaggtg tgctgacctc gaagcgtctt gaactgctgc gcgagcttgc
     4381 cccctcggca aggatcgccg ccttgatgaa tcccaataac tcgaatgcca aattcagatt
     4441 gaaggagatt gagaaggcaa gccgaatcct cgacttgcga gttgaaatcg taaatacacg
     4501 caatgcagat gagatcgatg gagcatttgc aagcctgctg cagcgaggcg ccggcgctct
     4561 tctggtcgtc gacgatcctc tctgtagagg cgcacgcagc aaactggtcg cgctggcaat
     4621 gcgtaaccgg attccaacga tgtatttcca aagtgaattt gtgcaggtcg gtggattgat
     4681 cagctatgga agcaactatg ccgagcggtt tcgccaagtg ggcatctaca cagcgcaaat
     4741 cctcaaaggg gccaaacccg ctgagctgcc ggttgtacag ccgacacggt ttgaactggt
     4801 gatcaacctc aggaccgcgc gagcgctcgg cctggacgtc ccgccgacgc tgctcgcccg
     4861 cgccgacgag gtgatcgaat gattgggcgg cgtcagttca tcaccctgct tggcggtgcg
     4921 gcggtggtcg ggccgctcgc ggcgcgggcg cagcaggcgg agcgggtgcg gcggatcggc
     4981 gtgctgatgg gcgctgtgga aagcgatccg gctaggcagt ctcagctcgc ggccttccga
     5041 ggtgcgctcg cgaaactggg atggagggaa ggtagcaatc tcgggatcga agttcgctgg
     5101 ggcggcgatc cggagctctg cgcgcgctac gccgcggagt tagtcgcgct cggccctgag
     5161 gtgattttgg ccgacgcctc cccgtctgtg gaggcgctgc aacggcaaac acgcacgatc
     5221 ccgatagtat tcgtcactgt cgccgacccg atcggccaag gctttgtcgc gaacctggcg
     5281 caccctggtg gcaacaccac cgggttcagt gttttcgacg caccgatggc gggcaaatgg
     5341 cttgagatgc tgacgcaaat cactccaccc gttacacgcg ttgcgatgtt gttcaatccc
     5401 gcgacgacgc cctatgccaa cctaatgttt cgggccatgg aggacgcagc gccgtctttc
     5461 gctgtagcgg tacgggccgc acccgttaac agtgagtccg aagtcgatgc catgatggcc
     5521 gcgctggcgc gcgaaggacg cggcggcgtg ctggttttga cgagcatctt caccgttacg
     5581 catcgcgatg ccattatcgc cctcgccgcc cgacatcgtc tacccgcagt ctatgcttac
     5641 tccttctttg ccgaggctgg cgggctgatg tcttacggcg tcgacgtcgc cgatgtgcac
     5701 cgccgcgccg ccgattacgt caatcgcatc cttaagggcg acaagccggg cgacctgccg
     5761 gttcagatgc cgatcaaatt caacctcgtg atcaacctga agaccgcgaa ggctctcgac
     5821 atcacagtcg cgacatcact cctcgctacc gccgacgagg tgatcgaatg aagaggcgcg
     5881 agttcatcac gctgctcggc ggcgcggcag ccacgtggcc gcttgcacgc cccgctgact
     5941 gatccgctac aatccgatat gctgcaccaa cagccatcat cgccggggta atgagatgca
     6001 gtttggtcaa ttcgggcggc gtgagtttat taaacttgtc ggcggcgcgg tcgttgcggg
     6061 gcctggcgct gcccttgcgc agacgcccgg ccgcctttgg aaaatcggtg ttctcgcgaa
     6121 cgagtcctgg ccaccgctgg agggattgcg ggacgggctg cgcgacctcg gctatgtcga
     6181 aggaaagact catctcttcg tgtatcgttt tgcg
//
LOCUS       DATLJT010000851         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3501685,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000851 DATLJT010000000
VERSION     DATLJT010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501685"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VM910_19435"
     CDS             <1..1081
                     /locus_tag="VM910_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI64734.1"
                     /translation="RRAALALALALGVAGAADLGYGYLTTGRYLETTDDAYVKADSTI
                     IAPKVSGYISEVLVADNEPVKAGQVLARIDDRDFKTALKQAHADVAAAEASVRNLNAQ
                     IELQEPLIQQQAAEVDAAEANLKFAEQERSRYDELMKSGSGTVQRAQQTDAALRAQTA
                     QLQQGKAGLIAANKKIEVLDTERAKAVAQLDHARAVEQQAALNLSYTQITAPVDGTVG
                     ARTLRVGQYVQAGTQLMAVVPLDAVYVVANFKETQLTHVRNGQPVEVRIDSFSATRLK
                     GHVDSLSPASGLEFALLPPDNATGNFTKVVQRVPVKIVLDDQSLRGLLRPGMSAEPTV
                     NTKATVVAEREAAKRLASRALEPRS"
     gene            1097..2716
                     /locus_tag="VM910_19440"
     CDS             1097..2716
                     /locus_tag="VM910_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HVI64735.1"
                     /translation="MTALQPAVDAIPASDRPQPLQAQAAPAVSAKTWLAVIAATLGAF
                     MAVLNIQIVNASLADIQGAIGAGIDDGGWISTSYLIAEIVVIPLSGWLAQVFSIRIYL
                     LTNAFLFLVFSAACALAQDLPQMIALRAVQGFAGGVLIPMAFTLIITLLPKAKQPIGL
                     ALFAVSATFAPAIGPTIGGYLTENFGWQYIFYVNLVPGALMIGMLYVSLEAKPMKLSL
                     LREGDWPGIVTMAIGLSALQTVLEEGNKDDWFGSPFIVRFAVIAAIALPLFVVIELTS
                     KKPLLNLRLLFRRNFGLGVFANFLLGVALYGSVYILPVYLTRIQGYNSEQIGMVLAWT
                     GLPQLVLIPLVPRLMKRVDPRIVIAIGFALFAASNFMNVYMTGDYASDQLFWPNVVRA
                     IGQALVLTPLSAIATGGIEPENAGSASALFNMMRNLGGAIGIAALQTLLTKREQYHSN
                     VLTQSVSMFEEATRTRIEQLTQYFMNHGVIDRVDAAHRAYVAVGNVVQKQAFILAFSD
                     TFYVLGAALIVALLAGLLLKKPDHMDAGGAH"
     gene            2752..>3116
                     /locus_tag="VM910_19445"
     CDS             2752..>3116
                     /locus_tag="VM910_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI64736.1"
                     /translation="MKTRMNFYQAAPDTIKALSALETQVQNSGLEQSLIELVKTRASQ
                     INGCAFCINMHTQDARKHGETEQRMYLLNAWREAPVYSDRERAALAWTEAVTLISETH
                     APDDVYEEVRKHFSEAETV"
BASE COUNT          571 a         1030 c          955 g          560 t
ORIGIN      
        1 caggcgggcg gcgctcgcgc tggcactcgc gctgggcgtg gccggtgctg ccgacctcgg
       61 ctacggctat ctgaccaccg ggcgatacct cgagacgacc gacgacgctt acgtcaaagc
      121 cgactccacg atcatcgcgc caaaggtctc cggttacatt tccgaggtgc tggtcgccga
      181 caacgagccg gtgaaggccg ggcaggtttt ggcgcggatc gacgaccgcg atttcaaaac
      241 cgccctcaaa caggcgcatg ccgacgtcgc cgccgccgaa gcctcggtgc gcaacctcaa
      301 cgcacagatc gaattgcagg agccgctgat ccagcagcag gcagcggagg tcgacgcggc
      361 cgaagccaat ctgaaattcg cggagcagga gcgcagccgt tatgacgagc tgatgaaatc
      421 cggttccggc acggtgcaac gcgcgcaaca gaccgacgca gcgctgcgcg cacagactgc
      481 acagcttcag cagggcaagg cagggctgat cgccgccaac aagaagatcg aggtgcttga
      541 caccgaacgt gcgaaggcgg ttgcgcagct cgaccacgcc cgcgcggtcg agcagcaggc
      601 ggcgctcaat ctgtcctata cgcagattac cgcaccggtc gacgggacgg tcggtgcgcg
      661 cacgctccgc gtcggacagt atgtccaggc cggaacgcag ctcatggcgg tggtgccact
      721 ggatgcagtt tacgtcgtcg cgaatttcaa ggagacgcag ctcacacacg tgcgcaacgg
      781 ccagccggtc gaggtccgga tcgacagctt tagcgcgacc aggctgaagg gccatgtcga
      841 cagcctgtcg ccggcgagcg gcctggaatt cgcgctgctg ccgcccgaca acgccaccgg
      901 caactttacc aaggtcgtgc agcgtgtgcc cgtgaagatc gtgctcgacg accagagcct
      961 tcgcggcctg ctgcgcccgg gcatgtcggc ggagccgacc gtcaacacca aggctaccgt
     1021 cgtcgccgag cgcgaagcgg caaagcgatt ggcctcccgc gcgctcgagc cacgcagcta
     1081 agccgcggag acgaccatga ccgcgcttca acccgccgtc gacgccattc ccgcgagcga
     1141 tcgtccgcaa cctctccagg ctcaggccgc cccggctgtg tcagccaaaa cctggcttgc
     1201 ggtgatcgcc gcgacgcttg gcgccttcat ggcggtgctc aacatccaga tcgtcaatgc
     1261 ctcgcttgca gacatccagg gcgcgatcgg cgcgggcatc gatgacggtg gctggatctc
     1321 cacctcctat ttgatcgccg agatcgtggt gatccccttg agcggctggc ttgcccaggt
     1381 gttttcgatc cgcatctacc tcctgaccaa cgccttcctg tttctggtgt tctccgcggc
     1441 ctgtgcgctg gcgcaagacc tgccgcagat gattgcgttg cgcgccgtgc aggggtttgc
     1501 cggcggcgtt ctgatcccga tggcgttcac gctcatcatc acgctcttgc ccaaggcgaa
     1561 gcagccgatc gggcttgcgc tgtttgcggt ctccgcgacg tttgcgcccg cgatcggccc
     1621 gaccatcggt ggttacctca ccgagaactt cggctggcaa tacatcttct atgtcaacct
     1681 ggtgccgggc gcgctgatga tcggcatgct ctacgtttcg ctggaagcaa agccgatgaa
     1741 gctgtcgctg ctgcgcgaag gcgactggcc gggtatcgtc accatggcga tcggactctc
     1801 ggcgctgcag accgtgctgg aagagggcaa caaggatgac tggttcggct cgcccttcat
     1861 cgtccgcttc gccgtcattg ccgcgatcgc actgccgctg ttcgtcgtta tcgaactgac
     1921 cagcaagaag ccgctgttga acctgcgcct gctgttccgc cgcaatttcg gcctgggcgt
     1981 tttcgcgaac ttcctgcttg gcgtcgccct gtacggctcg gtctatattc tgccggtcta
     2041 tctcacccgc atccagggct acaattccga acagatcggc atggtgctgg cctggaccgg
     2101 tctgccgcag ctcgtgctga tcccgctggt gccgcggctc atgaagcgcg tcgatccgcg
     2161 catcgtgatc gccatcggct ttgcgctgtt cgcggcctcc aatttcatga acgtctacat
     2221 gaccggcgac tacgcctccg atcagttgtt ctggcccaac gtggtgcgcg cgatcggcca
     2281 ggcgctggtg ctgacgccgc tgtcggcgat cgcgaccggc ggcatcgagc cggagaacgc
     2341 ggggtctgca tctgcgctgt tcaacatgat gcggaacctc ggcggcgcca tcggcatcgc
     2401 cgcgctgcag accctgttga ccaagcgcga gcagtaccat tcgaacgtcc tgacgcagtc
     2461 ggtttcgatg ttcgaagagg ccacccggac ccggatcgag cagctcacgc aatatttcat
     2521 gaaccacggc gtcatcgatc gcgttgacgc tgcgcatcgc gcctatgtcg cggtcggcaa
     2581 cgtcgtacaa aagcaggcgt tcatcctggc cttcagcgac accttctatg tgctcggcgc
     2641 agcgctgatc gtcgcgctgc tggcgggtct cctgctgaag aagcccgatc acatggacgc
     2701 gggcggtgcg cattagagcc cttggtcaac caacctcgaa ggaggaacac catgaaaacc
     2761 cgaatgaatt tctaccaggc cgctcccgac acgatcaaag cgctcagcgc gctcgaaacc
     2821 caggtgcaga acagtggcct cgagcagtcc ctgatcgaac tggtcaagac gcgcgcatca
     2881 cagatcaacg gctgtgcctt ctgcatcaac atgcataccc aggacgcgcg caagcacggc
     2941 gagaccgagc agcggatgta ccttttgaat gcctggcgcg aggcgcccgt ctatagcgac
     3001 cgcgaacgcg ccgcgctcgc ctggacggaa gccgtgacgc ttatctccga aacccacgcg
     3061 cccgacgacg tctatgagga ggtccgcaaa cacttctccg aggccgagac ggtgaa
//
LOCUS       DATLJT010000852         3179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1893371,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000852 DATLJT010000000
VERSION     DATLJT010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893371"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..512
                     /locus_tag="VM910_19450"
     CDS             342..512
                     /locus_tag="VM910_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64737.1"
                     /translation="MPHIRLRAFGPSLSVARLSVASLCVCIAAALLAFDAPAANAGKI
                     KMVLPDEKEART"
     gene            complement(605..1648)
                     /locus_tag="VM910_19455"
     CDS             complement(605..1648)
                     /locus_tag="VM910_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI64738.1"
                     /translation="MAKIKFDFVKTYHTRHGKLVNYVRRPGHKLIRLPGLFGSREFTE
                     AHAAALATDTPRVEIAERRTRAGSINAAVVGYLGSADFAVLKPISQKTYRRIAEVLRR
                     DHGDMPIGSLQRRHVVQMVERKKDTPSAARDFLKVLKLVLAYAISVGMREDDDNPCVG
                     VKVKMPKGGSGYRTWTEAEIAAYQKFYPLDSRPRLAMEVLLHTGLRAEDAVVLGRGHV
                     RNGEHHIVAQKTGTQPVLPITSELEAAINAAAPSEHITYLVSERTGRSYSAKRFGEWF
                     SEQCDRAGLKGLSPHGLRKRAAERMAERGVTAPEMKAFFGWESTAVADVYIKKANRAH
                     LSRNAAAKLRVVR"
     gene            complement(1635..1847)
                     /locus_tag="VM910_19460"
     CDS             complement(1635..1847)
                     /locus_tag="VM910_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64739.1"
                     /translation="MASGSKEVRRPRRPTLRQALEAAEKAGKTVKSSKQGPDGTITLI
                     FSNGDETTTNTDDWDAHLKELDRGKN"
     gene            2090..>3179
                     /locus_tag="VM910_19465"
     CDS             2090..>3179
                     /locus_tag="VM910_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64740.1"
                     /translation="MPLKVARRESLLLDQVLEAGNSPKVHREQLRMTTGDGLFTWKQA
                     VLGFYEVCMRERWNSKHSRTLGATMVFNYLLPGLDPKTIPITPKVKPPLAPWKWTNLP
                     LQATTHLHVYEILKTETPPYRKRKAERAGADMSPGPLFKTKPEMGKRIQCFLYGMYQY
                     WKARFKFKGENPANKESGSLLYELIGSFPRGGHLEDMRVDEVPPLIAFLHEPQRDPRF
                     ITSDQLATALGISPIAIKNARKRYGLKGHKEPGRMFKNASYVYEIAEAERVFKRPIPP
                     VTVRADELLYASMLEMAVLCLAREDMVAGMLWDEIKPRWENSVQGVILWHKHKTEHHG
                     YKCGTVITPRIQTILDRMWERRKQLGLKS"
BASE COUNT          600 a         1038 c          931 g          610 t
ORIGIN      
        1 gggggatcgg ggatgtatct cttggccatt ttccgagcca ttacagccgc cttgacgcac
       61 cgccgctatc ggcctgagct gcactacatg cgcggtccgg gaccgaagtg gttcgagcgt
      121 cacggccgat agtcctgttc gcgcaagcct cggttggctt cgtccgtgcc ggtctcaacg
      181 acgacacggg cgcgataggc agttaactcc aaaacaatcc tgccggcggg ggctgcgccg
      241 cgtccgcaga tcgcgcggtc cgagaaataa tttgcatgac gtgggcgaaa ggctcatttt
      301 tcgctgccga tgatcgcttg tcggcctggc ggggagttgt catgcctcac attcgattgc
      361 gcgcattcgg cccaagcctt agcgtcgcaa ggctcagcgt cgcaagcctt tgcgtttgca
      421 tcgccgcggc gctgctcgcc ttcgacgcac ccgccgccaa tgccggcaag atcaagatgg
      481 tgttgccgga tgaaaaagaa gcccgcacgt agcgggcttt tttgcgtcgg tgagacacgt
      541 ccggcggcga ggtgagacaa tcgatgttgc caattcattc cggttcccca ttcgaccgcc
      601 gcgatcagcg gaccaccctg agcttcgccg ccgcgttgcg ggacaggtgc gcgcggttag
      661 ccttcttgat gtagacatcc gccaccgccg tcgactccca accgaagaac gccttcatct
      721 cgggagcagt caccccgcgc tcggccatgc gctcggccgc ccgcttgcgc agaccgtgcg
      781 gtgagagccc tttaagcccc gcgcggtcgc actgctcgga aaaccattcg ccaaaccgct
      841 tggcgctgta actgcgtccc gtgcgctcgc tgacgaggta agtgatgtgc tcgctcggcg
      901 ctgccgcgtt gatggcggct tccaattccg aggtgatcgg gagcacgggt tgcgtgccgg
      961 tcttctgcgc gacgatgtga tgctcgccgt tccggacgtg accgcgtccc agcacgaccg
     1021 cgtcctcggc ccgcaatccc gtgtgcagca ggacctccat ggccagcctc ggcctgctat
     1081 cgagcgggta gaacttctga taggcagcga tttccgcttc ggtccaggtc cgatacccgc
     1141 tgcccccttt cggcatcttc accttcacgc cgacacaggg attgtcatcg tcttcgcgca
     1201 tgccgacgct gatcgcgtac gcgaggacca gcttcaacac cttcaagaag tcgcgcgccg
     1261 ccgacggcgt gtccttcttg cgctcgacca tctgcaccac gtgccgacgc tgcaagctgc
     1321 cgataggcat gtcgccgtgg tcgcgcctga ggacttcggc gatgcgccgg tatgtcttct
     1381 gcgagatggg cttgaggacg gcaaaatccg ccgacccgag gtagccgaca acggcggcat
     1441 tgatgctccc cgcccgcgtt cgtctttctg cgatctccac gcgcggcgtg tcggtggcga
     1501 gtgccgccgc gtgggcctcc gtgaactccc tggagccgaa cagccccgga agccgaatca
     1561 acttgtgccc ggggcggcgg acatagttca ccagcttgcc gtgccgagtg tggtaggttt
     1621 tgacgaaatc gaatttaatt tttgccacgg tccagttcct tcaagtgggc gtcccagtca
     1681 tcggtgttgg ttgtcgtctc gtcgccattg ctgaagatga gcgtgattgt cccgtccgga
     1741 ccttgcttcg aggacttgac cgtcttgcca gccttctcgg cagcctcaag cgcctgccgc
     1801 aatgtcggcc tacgcggacg acggacctcc ttgctgccgc tggccatttg cggttctcac
     1861 gcgaacgatt attcgaagaa gtctcccaga acagcttgat aagggcatta taggccggtg
     1921 gcctataggc accgtcaccg agcccggtcg ctacaacttc ggcgacggcc tcttcctcga
     1981 cgtcaagccg ctcgccgacg gcgtcggcaa atactggctc tacgcctaca acggcaccaa
     2041 agtcggcgag agcgcacgcg agcgcatcgg ctttgcctcc gccaaaacca tgccgctcaa
     2101 ggtcgcccgc cgcgagtcgc tcctgctcga ccaagtgctc gaagccggta actcgccgaa
     2161 ggtccatcgc gagcaactgc gcatgaccac aggcgatggc ctgttcacct ggaagcaggc
     2221 ggtcctcggc ttctacgagg tctgcatgcg cgagcgctgg aacagcaagc actcgcgcac
     2281 cttgggcgcg accatggtgt tcaactactt gcttccgggt ttggacccca aaacgattcc
     2341 gatcacgcca aaggtcaaac ctcctttggc tccgtggaaa tggacgaacc tgccgctaca
     2401 agccaccacc catcttcatg tctacgaaat ccttaagacg gagacgccac cctaccgcaa
     2461 gcgcaaagcg gagcgcgcgg gggccgacat gtcacccggc ccgctgttca aaacgaagcc
     2521 cgagatgggc aagcggatac aatgcttcct ctacggcatg taccagtact ggaaggcgcg
     2581 ctttaaattc aaaggcgaga acccggccaa caaagaatcc gggagcctgc tttacgaact
     2641 gatcggcagc ttccccagag gcgggcatct tgaggacatg cgcgtcgacg aagtcccgcc
     2701 gctgattgca ttcctgcacg agccccagcg cgatccgcgt ttcatcacct ccgaccaact
     2761 cgcaaccgcg ctcggcatat cccccatcgc tatcaaaaat gcgcgcaagc ggtatgggct
     2821 caaaggccac aaggaacccg gacggatgtt taaaaatgcc agctacgtct acgagatcgc
     2881 cgaagccgaa cgtgtgttca agcgtccgat cccaccggtt accgtgcgtg cggacgaact
     2941 gctgtatgcg agcatgttgg agatggcagt cctctgtctt gcgcgcgagg acatggtggc
     3001 tggcatgcta tgggacgaga tcaagccgcg ctgggagaac agcgtgcagg gcgtgatcct
     3061 ctggcacaag cacaagaccg agcatcacgg ctacaagtgc ggcaccgtca tcaccccgcg
     3121 catccagacc atcctcgacc gcatgtggga acgccgcaag caactcggcc tcaagagcc
//
LOCUS       DATLJT010000853        17773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3042078,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000853 DATLJT010000000
VERSION     DATLJT010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042078"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VM910_19470"
     CDS             complement(<1..398)
                     /locus_tag="VM910_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846962.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVI64741.1"
                     /translation="MTTATDAASVSKPFQAEVSELLHLMVHSVYSETDIFLRELISNA
                     SDACDKLRYEAIARPELIGDGEAPKIRIAPNKKADTLTVSDTGIGMDRQELIDNLGTI
                     ARSGTKDFVAKLAEAKDGASLIGQFGVGFYS"
     gene            complement(504..1706)
                     /locus_tag="VM910_19475"
     CDS             complement(504..1706)
                     /locus_tag="VM910_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64742.1"
                     /translation="MKSSYRWVIVACGALMTCVGIGAMFSLAVYLQPMSVDTGWSRTG
                     ISSAMTLDFLVMGAAGFGWGALSDRIGPRLVVLAGAVLLGLGLVLASGATSLLQFQLT
                     YGFLVGLASGAFFAPMIAAATDWFEDNRSLAVSLVSAGMGVAPMTISPFARWLISAYD
                     WRTAMMVIGMVAWSLLVPAALFVRRAPRPVAGAGAAPSPTEPSLSAIEAFRSPQFVVL
                     AAAFFLCCAAHSGPIFHMVSYAIGCGIAPLAAVSIYSVEGLSGLGGRLLLGLLADRFG
                     AKRVLVAGLLVQAFAIASYLMVSRLGEFYLLSVVFGTAYGGVMPLYAVLARESFGQRV
                     MGTVFGAMTMVSAVGMAFGPWAGGTIFDAFNSYMWLYVGAAAIALGAVAVACTFAGRP
                     AVQRLQAA"
     gene            complement(1821..2216)
                     /locus_tag="VM910_19480"
     CDS             complement(1821..2216)
                     /locus_tag="VM910_19480"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI64743.1"
                     /translation="MVDTGELYARGLARRKKLFGEAEVEKRMAASGEFGAPLQNVINA
                     YVYGDVWERKGLSDAERSLVMLAITAAANRPAEFRVHAKGARANGCSPAQVQDLLLLV
                     AMYCGIPAAIETHQIAAEIFGENPGAAAP"
     gene            2352..3314
                     /locus_tag="VM910_19485"
     CDS             2352..3314
                     /locus_tag="VM910_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64744.1"
                     /translation="MRPRSGVFACLALLVLTAQQAFAADDDYPRRQIKIIVPFPGGAG
                     PDQVARLLGKHLQDAFGQPVVIENRAGALGSIGAAEVAHSAPDGYTLLMGTNTTQASN
                     VATLKNLAYDPAKDFSPIIRTTTTAMVLMVKPDFPARDLSEFLNYAGAHHLTAGYGSG
                     ASQISVAQLQSRGGMTLVAASYRGVPQAMTDVIAGVIDLTFGDFSVAIPQMQGGTLRG
                     IGVTSPKRNELLPDLPALAEAMPGFEATIWYGLLAPANTPEPIVRKLHAESEKFLSQT
                     ETKQRLANVGVIVATQSPAEFRKFIESEIVRWTADCKAAGIEPQ"
     gene            3380..4354
                     /locus_tag="VM910_19490"
     CDS             3380..4354
                     /locus_tag="VM910_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVI64745.1"
                     /translation="MIRCAIAGLGRWGRALVEAASGNPGLMISRAVETDLESASAYCA
                     DRGIALADDFDDVLDDPAIDAVLLATPHSLHKAQVIAATAARKQVFCEKPLALRRADA
                     REMFEAARRTNVVLAVGHNRRFWPSMRALRTIVTSGELGRILHIEGHNSNENSNSILR
                     GWRLSQEESPGGGLTGAGLHVLDAFTGLAGPVRRVYARLSSREAGPPPLDSVMLALDF
                     ANGVTGTLATVRATPFYWRVHVFGAKGSAEVLDDVTMVVRKSGHPPQQIKYPAIDTLA
                     AELDAFADAILEKRPFPVPEAEVLATLAAFEAALASMRSGKAVACNAR"
     gene            4344..5099
                     /locus_tag="VM910_19495"
     CDS             4344..5099
                     /locus_tag="VM910_19495"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI64746.1"
                     /translation="MRVEAPKRKSARYRDIATKLQKEIRLGRYDIGQLLPTETELMAR
                     HSASRQTVREALRILMDQGLIVRRAGLGSVIIASEPPVLFTHSVKSLAEWLRYSNETY
                     REVVAHRDIIADRALAALLKCEPGKHWFLIEAVRRSDQFAAPLGWVEIYVLRKFAPVV
                     KRSDHGRTPVHEQIAKMFGQTTEYAQMEIFARGMPAPLAKALQVKAGSPALTVVRRYY
                     GAGEELLEVTVTTHPEGRYTYTMDMLRSQRPRV"
     gene            5278..6492
                     /locus_tag="VM910_19500"
     CDS             5278..6492
                     /locus_tag="VM910_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HVI64747.1"
                     /translation="MSPSTRANLAAYGRDLRLDLVRGIANWSIFLDHIPHDAVNLLTV
                     RNFGFSGAADLFVFTAGYAAAILYGRMTLERGILVAVTRILKRFWQLYAAYLVLFVVY
                     IDVIGSVAAQYAAPDIIDEYKVIGIVDDPTRTLLHGLLLKAVPLNLDALQLFIVLMAG
                     LSIVLAALLRWPNLTMAGSLALYAAAREFDLHPWSVPDGHWFFNPLCWQLLFVLGAWL
                     ALKGTNHVRAMQEIPLLRQLALAYLVLALLVTVAGKIPQLGALLPDALMSPFTPNDRE
                     SLPPFRVLHLLALVFLASRLVPPDWEGLRRKALQPLIKCGEEWLPCFCAGVFLSFAGH
                     FVLITGPNSLVMQVLVSAAGIVMMTTVAYYVSWSRQQDGMSSRGAEAVGGFTQWPTDT
                     AERKHQTEPSRR"
     gene            complement(6431..7234)
                     /locus_tag="VM910_19505"
     CDS             complement(6431..7234)
                     /locus_tag="VM910_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI64748.1"
                     /translation="MPSWTCFQCGAQFPETASPPPSCPICEDERQFVNWRGQQWLTRE
                     ELAQRYRLMLRDDLGVTGVGVEPSLAIGQRALLLREADGCVMWDCLPLATLDAVQQVR
                     VLGGLKAIAVSHPPFYGAVVDWSEAFGNVPIYLHGDDRKWITRPHPAIVCWTGDSHRI
                     SGDIALMRGGGHFPGATMLHWRAAANGKGALFVGDIAMVAMDRQHVSFMYSYPNLIPL
                     NASAVRRIADAVAPLAFDRIYGAWWDRNIATDAKARFDASVQRYLSAIA"
     gene            7326..7712
                     /locus_tag="VM910_19510"
     CDS             7326..7712
                     /locus_tag="VM910_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HVI64749.1"
                     /translation="MNVGIICTALLGLLLFGLGLYVSILRGRTRRSIGCDTNPTDPLH
                     RAVRAHGNTAEYAPFLGLLFLWFAMHPASSWVAGAIVIATLARFLLVAGLLWGSSLDK
                     PNAARFLGALLTYVCGLALVVRLAVA"
     gene            complement(7818..8987)
                     /locus_tag="VM910_19515"
     CDS             complement(7818..8987)
                     /locus_tag="VM910_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI64750.1"
                     /translation="MTRQPKQAAGSSGREEIRAGDLFLLPLAGAQAMLDAWLGWFGDH
                     RKPPFDDEAALPWTMPHRVTLELTTLRLRDFSAQKAGRPVLICAPYALHSALITDFAP
                     AHSIVQALQQGGVSRVHVTDWRSATPQMRSLSIDSYLADLNVAIDEIGPPVDLVGLCQ
                     GGWLSLVYAARFPEKVRRLVLVGAPVDVSCQSPLSQMAATLPHAAFAGLVNATTGLVS
                     GLQLRSVWSAALNAPDEAVRQSFADANDGGHLLARFQRWNDATLDLPGVYYVEVADRV
                     FRENRIAKGGFVALGRRIDLRRFTLPVFLLVGTEDRVVPAEQALATAGLLGTPAAQVE
                     IAVEPCGHLGLFMGEAALNRGWRRIAAWLCADEPSRSLDQAPPRDFAAVAASSRA"
     gene            9112..9327
                     /locus_tag="VM910_19520"
     CDS             9112..9327
                     /locus_tag="VM910_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HVI64751.1"
                     /translation="MPYRTNDDLPPAVRNHLPAHAQDIYREAFNHSFTSHSGDPRQEE
                     IAHRTAWAAVKRLYAKVGDNWMPLPGH"
     gene            9598..10104
                     /locus_tag="VM910_19525"
     CDS             9598..10104
                     /locus_tag="VM910_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007531543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64752.1"
                     /translation="MKRLVTVNDHMQKGYKYRLVAPIGRNFDPEFDPDLTPKDMLRLG
                     VFGGKYMTDCRKEFPHNWFVRAKLSPKRADPALNFFGVTASQPLAEWRRKGWIHPDDP
                     RGWFQWYCRYYMGRRMPQEDARQIKRWKAIRRHVRQIQQHCERGDLLCRRRQRQALLH
                     WAYDSRKL"
     gene            complement(10133..11578)
                     /locus_tag="VM910_19530"
     CDS             complement(10133..11578)
                     /locus_tag="VM910_19530"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HVI64753.1"
                     /translation="MAIRAPAHNRARFAVTVLSLVCAFRAAGYAASEVADKVSLSEAT
                     IRWSTVKYATNTDNGFVEGSLDKTTIVDRTFKTRVLENRYLKVTLVPEFGGRILSIIY
                     KPTGHEELYRNEVGVPYGINAGNFYYDWLMVYGGVFPTFPDAEHGKTWLKPWDFKVVN
                     ESAGEVTVSMSLKDDFAYSAAPRQFFRGSTGLEATYYVTLKADRAALDARVVLKNPQA
                     RTIKYEYWTCTTLAPGSDPNNPKATGGAEIIAPIKAYQTPHWSKNLADGEESAGGDTS
                     RFEKLRYFKNWPTMGIAYAAPDMQGGNFWGVINHDNEEGIIRIADNKVTPGLKMWTWG
                     FPSFTNQTDARKDPNEAQPYVELWAGVSDQFFHSADFPAAGEVTIPETYSPTVGISNV
                     THANDNMLVNLSAEASRVNLQFFSLEPATPLRVTLKRGDAVLFDDDVRADPKNGNRVS
                     AAIPAGNGEQVRLTIRTAEGKELIAAETKIK"
     gene            complement(11707..12006)
                     /locus_tag="VM910_19535"
     CDS             complement(11707..12006)
                     /locus_tag="VM910_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVI64754.1"
                     /translation="MPTQLSKSQLIERIATSTELSKRDVKNVMEMLVDVGHKELKKNG
                     IFLVPGFAKFVVVKKPATKARKGVNPFTGEEMMFKAKPARKIVRARPVKAAKDAV"
     gene            complement(12329..12775)
                     /locus_tag="VM910_19540"
     CDS             complement(12329..12775)
                     /locus_tag="VM910_19540"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64755.1"
                     /translation="MIRAPTIEELPSLSELCLRSKATWGYNREFLEACRSELSFDQRD
                     LQLTTIAVAELGGKVVGLAQVKVTGDEADLLKLFVEPSALHRGVGRALFAWATHVAKK
                     RGATRLIIDSDPGAAPFYRRMGAYDLGQAPSGSIPGRILPKLAIGV"
     gene            complement(12953..13156)
                     /locus_tag="VM910_19545"
     CDS             complement(12953..13156)
                     /locus_tag="VM910_19545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64756.1"
                     /translation="MVYTSDFSEVLMGLAHYLDLILRGANPGDLPYLQPTRFQFAVNL
                     KAARAIELDIPAALLASADKVIE"
     gene            13418..14035
                     /locus_tag="VM910_19550"
     CDS             13418..14035
                     /locus_tag="VM910_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI64757.1"
                     /translation="MSGIVYVVDDDAFFRAAIQQQLEQTGYRVVTYASAEEVLEQRPD
                     ENGPGCILLEVRMPGLSGPALQSRLLELGSTLPILFITGYADVSTTVKALKAGADDFL
                     IKPVQSDELLRAIRRSLARHQTALEVKREFDVLRGRLATLTPRERQVFLLIVRGKINK
                     QVANELGATERTIKAHRHNVMEKMKVRSLAELVLIGERLGVSARD"
     gene            complement(14053..14820)
                     /locus_tag="VM910_19555"
     CDS             complement(14053..14820)
                     /locus_tag="VM910_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI64758.1"
                     /translation="MEFRVKGDAAYLALHDSIRSDGETAIDGGSRSTLTDLRDKLTFV
                     PIGSSVGGWNRFEGRASSVFAVHLTSTRSDHDANDISKIPSALYFENNNLRATLQKLQ
                     SVLDGSGINDRAYAETLGLLLLWELRHAADPGHLRLNPVRGGLTARQLRRVKEFIEAQ
                     IPNEITISDLAALAGLSHYHFIRSFKDTVGLTPYQYVLSERIRRARGLLSKPDLSLGD
                     VALAAGFSDASQLNRVFRKFVGVTPTAFRRETGSRFP"
     gene            complement(15170..15925)
                     /locus_tag="VM910_19560"
     CDS             complement(15170..15925)
                     /locus_tag="VM910_19560"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI64759.1"
                     /translation="MSENVLPPDEHAIRVDLAACYRLVALYGWDDLIFTHISARVPGP
                     EHHFLINPYGLMFEEITASSLVKIGLDGNPVGKSNYPVNPAGFTIHSAVHEVREDAGC
                     VMHLHTPDGTAVSTSKERLLPLNQTAQLVLPDLAYHDYEGVALDYDERPRLQRDLGQK
                     NLMLLHNHGTLAVGKNCAEAFLRMYLLERACTMQVRTRMLGDVSMDHPTDEAVIEKNA
                     ELTRGGGLSSLADTLVWPALLRKLDRAGSDFRN"
     gene            16315..17619
                     /locus_tag="VM910_19565"
     CDS             16315..17619
                     /locus_tag="VM910_19565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI64760.1"
                     /translation="MRRWRPDRLEVARWALCVLIAALAVANAAFEKAWARDTGSLSRA
                     DLRSVSDFMRHEVATGQIAGAIVLIQQHGHPVYFEKFGLRDIGPKLPITDDTIFRLYS
                     MSKPVTSVAAMMLVDDGKLSLDDPVAKYIPAFAHLEVGVERHDRTGKAVLTREPLKRP
                     ITILDLLRHTSGLTYGFYGESAVRKLYAKADLYTGDFDNAEFVTRLAKLPLAEQPGTV
                     WDYGVSTDVLGRVIEVVSGQSLFQFEKERLLDPLGMTHTAFYVTDEAKRELIAQFLPK
                     ETWKAFIGIREPMERTPWESGGAGLTGTVGDYARFAQMLLNGGTLDGRRYLKPETVAR
                     MTSDNIGPGGGIAHTHLYFLSTESGFGLGFAVLTAKPRNKPWPPGEYGWAGAAGTFFF
                     VDPKNDLFVLCMMQTHLHRGRIEGELARLVYEATARQAVSSR"
BASE COUNT         3511 a         5527 c         5308 g         3427 t
ORIGIN      
        1 gaatagaaac cgacaccgaa ctggccgatc aggctggcgc catccttggc ttccgcaagc
       61 ttcgccacaa aatctttggt gccggaacgg gcgatggtgc cgagattgtc gatcagctcc
      121 tgccggtcca tgccgatgcc ggtatcgctc accgtcaggg tgtcggcttt cttgttcggg
      181 gcgatacgga ttttcggtgc ctcgccgtcg ccgatcagtt cgggcctggc gatcgcttca
      241 tagcggagct tgtcgcaggc gtcggacgcg ttcgaaatca gctcgcggag gaagatgtcg
      301 gtctcggaat agaccgaatg caccatcagg tgcaaaagct cggagacctc ggcttggaac
      361 ggttttgaca cggaggcggc atcggtcgcg gtggtcatta tgcgctcgtt cggtcgacaa
      421 acgaaatggg gagaccccgg atatagcaat gccggcggcg cccgcaagat agggcgggcg
      481 cccaagccgt cgcgaccgta agctcatgcc gcctgcaacc gttgcacggc cggccggccc
      541 gcaaaggtgc aggccaccgc caccgccccg agcgcgatcg cggcggcgcc gacatagagc
      601 cacatgtagc tgttgaaggc gtcgaagatc gttccgccag cccagggacc gaacgccatc
      661 cccacagccg acaccatggt catggcgccg aacaccgtcc ccatcacgcg ttggccgaag
      721 gattcccgtg ccagcaccgc atagagcggc atcaccccgc cataggccgt gccgaaaaca
      781 accgagagca ggtagaactc gccgagccgg ctgaccatca ggtagctcgc gatcgcaaag
      841 gcttgcacca acaaccccgc gaccagaacg cgcttggcgc cgaagcggtc cgcgagcaga
      901 ccaagcagga gacgtccgcc gagacccgac aatccctcca cgctgtagat cgaaacggcg
      961 gcgagcggag cgatgccgca gccgatggcg taactcacca tatgaaagat cggcccggaa
     1021 tgcgccgcgc aacaaaggaa gaaggccgcc gccaatacaa cgaactgcgg cgagcggaac
     1081 gcctcaatcg ccgatagcga aggctccgtc ggtgacgggg ccgcgcccgc gccggcaaca
     1141 ggcctgggcg cacgacgcac aaacagcgca gccggaacca gcagcgacca ggcgaccatc
     1201 ccgatcacca tcatcgcggt gcgccagtcg taagcggaga tcagccagcg cgcaaagggc
     1261 gagatggtca tcggcgcgac gcccattccg gccgaaacca acgatacggc tagcgaccgg
     1321 ttgtcctcga accaatcggt ggccgccgcg atcatcggcg caaagaaagc gcccgaggca
     1381 aggccgacca ggaacccgta agtgagctga aactgcagga gcgaggttgc gccgctcgcg
     1441 agcaccagcc ctagaccaag cagtaccgcg ccggccagca ccacgagccg cggcccgatg
     1501 cggtcgctga gcgcgcccca gccaaaaccg gcagcgccca tcaccaggaa atcgagcgtc
     1561 atcgcgctcg aaatgccggt tcgcgaccag ccggtatcga ccgacatcgg ctggagataa
     1621 accgcaagcg agaacatcgc gccgatgccg acgcaggtca tcaacgcacc gcaagccacg
     1681 atcacccagc gataggacga tttcatgctg atctcctctt tcgtctcgta gttccaagac
     1741 gaacggggtt ccgcatttcc gacatcgtcg gacaaaaaat gagcggctcg gccgcgtcgc
     1801 cctacgctgc ggcgcttggc tcagggcgcc gccgctcccg ggttctcgcc aaagatttcc
     1861 gcggcaatct gatgggtttc gatcgcagcc ggaattccgc aatacattgc gaccagaagc
     1921 agcaaatcct gtacctgcgc cgggctgcat ccgtttgccc tcgcgccttt ggcatgcacg
     1981 cggaattccg ccggccggtt ggccgccgcc gtgatcgcga gcatcaccag actgcgctcg
     2041 gcatcggaca ggcctttccg ctcccagacg tcgccataaa cataggcatt gatgacgttc
     2101 tgcagcggcg ccccgaattc ccccgacgcc gccatccgct tctcgacttc ggcctcgcca
     2161 aacagttttt tgcgccgggc cagcccgcgc gcgtagagtt cgcccgtatc caccatatcg
     2221 cgcctcataa cggcttctgc ctcccgaatt atacggataa atcagaaagg ctcaatcccc
     2281 ggccatacag ttgcgtccgc ttgacatatt tgtccgtata aagtcgaaag tagtttctgg
     2341 ggagcgagac gatgcggccg cgttcgggag ttttcgcctg cctggcattg ctggtgctga
     2401 cggcacagca ggcgttcgcg gccgacgatg attatcccag gcgccaaatc aagatcattg
     2461 tgccgtttcc aggcggtgcc ggccccgatc aggtcgcccg cctactcggg aagcacttgc
     2521 aggatgcgtt cggccagccg gtggtgatcg agaaccgggc gggcgcgctc ggcagcatcg
     2581 gcgccgccga agtcgcccac agcgcgccgg acggctatac acttttgatg ggcaccaaca
     2641 cgacgcaggc gagcaacgtc gcgacgctga aaaacctcgc ctacgacccg gccaaggatt
     2701 tttcgccgat catccgcacc acgacgacgg cgatggtcct catggtcaaa cccgatttcc
     2761 ctgcaaggga cctctccgaa tttctgaatt atgcgggtgc ccaccacctg actgcgggct
     2821 acggctcggg cgcctcgcaa atctcggtcg cgcaactgca atcgcgcggc ggcatgacgc
     2881 tcgtggccgc atcctaccgg ggcgtgccgc aggccatgac cgacgtcatc gcgggcgtga
     2941 tcgatctgac cttcggggat ttctcggtcg cgatcccgca gatgcagggc ggcacgttgc
     3001 gcggcatagg cgtcacctcg ccaaagcgca acgagcttct tccggatctg cccgcgctgg
     3061 cggaagcgat gccgggcttc gaagccacga tctggtacgg actgctcgcg cccgccaata
     3121 cgcccgagcc catcgtcagg aaactccatg cggagagcga gaaattcctg tcgcagacgg
     3181 agaccaaaca acggctcgcc aatgtcggcg tcatcgtcgc cacccagagc cctgccgaat
     3241 tccgcaaatt catcgaaagc gagatcgtgc gctggaccgc ggactgcaaa gccgccggca
     3301 ttgaaccgca atagatcatg accgctcacg ccgtcgaagc gatccggccg tcccctgccc
     3361 gatccgaagg agccgtcaga tgatccgatg tgccatcgcc ggcctgggcc gctggggccg
     3421 cgcccttgtc gaagccgcga gcggcaaccc cggcttgatg atttcgcgcg ccgtcgagac
     3481 cgatcttgag agcgcaagcg cctattgcgc cgaccgcggc atcgcgctgg ccgacgattt
     3541 cgacgatgtt ctcgacgacc ccgcgatcga cgccgttttg ctcgcaaccc cgcactcgct
     3601 gcacaaggcg caggtgattg cagcgacggc tgcgcgcaag caggtttttt gcgaaaagcc
     3661 gttggcgctt cgccgcgccg acgcgcggga gatgttcgag gcggcccgca ggaccaatgt
     3721 cgtgctcgcg gtcgggcaca acagacgatt ctggccctcg atgcgcgcgc tgcgcacgat
     3781 cgtcacgagc ggcgagctcg gccggatcct ccacatcgag ggccacaaca gcaacgagaa
     3841 ttcgaacagc atcctccggg gctggcggtt gtcgcaagag gaatcgccgg gcggtgggct
     3901 caccggcgca ggccttcacg tgctcgacgc cttcaccggc ctggcagggc ccgtgcgccg
     3961 ggtctacgcc cggttgtcgt cgcgcgaagc gggcccgccg ccgctggatt cggtcatgct
     4021 cgcgcttgat ttcgcgaatg gcgttaccgg cacgctcgcg accgtgcgcg ccacgccgtt
     4081 ctactggcgg gtccacgtct tcggcgccaa aggctctgcc gaggtgctgg acgacgtcac
     4141 aatggtggtt cggaagtccg gtcatccgcc gcaacagatc aaatatcccg cgatcgatac
     4201 gctcgccgcc gagctcgacg ccttcgccga cgcgattctg gagaagcggc cgtttcccgt
     4261 gccggaggcg gaggtgctgg cgacgctcgc ggcgttcgag gcggcgcttg cctcgatgcg
     4321 gtccggaaaa gcggtggcct gcaatgcgcg ttgaggcgcc caagcgaaaa tcggcgcgct
     4381 accgcgacat cgccacgaaa ttgcagaagg agatccggct cggccgttac gatatcggcc
     4441 aacttctgcc gaccgagacc gaattgatgg cgcgccattc cgccagccga cagactgtcc
     4501 gcgaggcgct gcggatcctg atggatcagg ggctgatcgt gcggcgcgcc ggcctcggct
     4561 ccgtgatcat cgcgtccgag ccgccggtgc tgttcaccca cagtgtcaag tcgctcgccg
     4621 aatggctgcg ctattccaac gaaacctatc gcgaggttgt cgctcaccgc gacatcatcg
     4681 ccgaccgcgc gctcgccgcg ctgttgaaat gcgagcctgg caagcattgg ttcctgatcg
     4741 aagcggtcag gcgatccgac cagttcgcag cacccctggg atgggtcgag atctacgtgc
     4801 tgcgcaagtt cgcgcccgtg gtgaagcgca gcgaccatgg ccgtacaccg gtgcacgagc
     4861 agatcgcgaa aatgttcggc cagaccaccg aatacgccca gatggaaatt ttcgcccgcg
     4921 gcatgccggc gccgctcgcc aaggccctgc aggtcaaggc gggctcgccg gcgctgacgg
     4981 tcgtgcgccg ctattatggc gcgggcgaag agctgctcga ggtcaccgtg accacgcacc
     5041 ccgaagggcg ctacacctac acgatggaca tgctgcggtc gcagcgtccg cgcgtgtgag
     5101 cggatcaatc gaaggtcaac gctgctttcg cgaatgtgat cgcatggcgg ctctgcattt
     5161 ggccgcatca cgtgtgcatt cacaaaaacg ctattcggcc cgccctgccc aaggaactac
     5221 atttgctggg cgattcgttt cgattagttg tttttctgtc cggcaaagtt cttccaaatg
     5281 agtccatcga cgagggccaa cctcgctgcc tatggccgcg atctccggct cgatcttgtc
     5341 cgcgggatcg ccaactggtc gatcttcctg gatcacatcc cgcacgatgc cgtcaatctg
     5401 ctgacggtgc gtaatttcgg attcagcggc gctgcggacc tcttcgtctt caccgcgggt
     5461 tacgccgcgg cgatccttta tggcaggatg acgctggagc gcggcatcct cgtcgcggtg
     5521 acccgcattc tcaagcggtt ctggcagctt tacgctgcct atctcgtgct gttcgtggtg
     5581 tatatcgacg tgatcggatc ggttgccgcg caatatgcag cgcccgacat catcgacgaa
     5641 tacaaggtga tcggcatcgt cgacgatccg acgcggacat tgctgcatgg cctgctgctc
     5701 aaggccgttc cgctcaacct tgacgcattg caactgttca tcgtgctgat ggcgggcctt
     5761 tcgatcgtct tggccgcgct gttgcgctgg cccaatctga caatggccgg atcgcttgcg
     5821 ctgtatgccg cagcccgcga gttcgacctg catccgtggt ccgttccgga tggacactgg
     5881 ttcttcaatc ccctctgctg gcagcttttg ttcgtgctcg gcgcctggct ggcgctgaag
     5941 ggcacgaacc acgtgcgcgc gatgcaggag atcccgctgc tgcgccagct tgcgctcgct
     6001 tacctggtgc tggctcttct ggttacggtg gccggcaaga tcccgcagct cggcgcactg
     6061 ctgccggatg ccttgatgag ccccttcaca cccaatgaca gggagagctt gccaccgttc
     6121 cgggtgctgc atctgctggc gctggttttc ctggcgagcc ggctggtgcc gccggactgg
     6181 gagggccttc gccgcaaggc gctgcagcca ctcatcaaat gcggcgaaga atggctgccc
     6241 tgcttctgcg ctggtgtgtt cctctccttt gccgggcatt tcgtgctgat caccgggccc
     6301 aattcgcttg tcatgcaggt actggtcagc gccgccggaa tcgtcatgat gacgacggtt
     6361 gcctactacg tctcctggtc gcgccagcaa gacgggatgt cctcgcgcgg agctgaggct
     6421 gtgggcggat ttacgcaatg gccgacagat accgctgaac ggaagcatca aaccgagcct
     6481 tcgcgtcggt agcgatgttg cggtcccacc aggcgccgta gattcggtcg aaggccagcg
     6541 gcgcgaccgc atccgcaata cgccgcaccg cgctagcgtt gagcgggatg aggttcgggt
     6601 agctgtacat gaagctcacg tgctgccggt ccatcgcgac catcgcgatg tcgccgacga
     6661 acagtgcgcc cttgccgttt gctgccgccc gccaatgcag catggtggcg ccgggaaaat
     6721 gcccgccgcc acgcatgagc gcgatgtcgc ccgaaatccg gtggctgtcg ccggtccagc
     6781 acacgattgc aggatgcggc cgcgtgatcc atttgcgatc gtcaccgtgc aggtagatcg
     6841 ggacgttgcc gaacgcctcg ctccagtcga cgaccgcgcc gtaaaaaggc ggatgcgaga
     6901 ctgcgatcgc cttcagcccg ccaagcacac ggacctgctg cacggcgtcg agtgtcgcca
     6961 gcggaagaca atcccacatc acgcagccgt cggcctcgcg caacagcagc gcccgctggc
     7021 cgatggcaag gctcggctcg acgccgacgc ccgtgacgcc gagatcgtcg cggagcatga
     7081 gcctgtaacg ctgcgcgagt tcctcgcggg tgagccattg ctggcctctc cagttgacga
     7141 attgtcgctc gtcttcgcaa atcgggcacg agggcggcgg gctcgcggtt tccggaaact
     7201 gcgcaccgca ttgaaagcag gtccaggaag gcatcggcaa tcacctcatc gaaacgaccg
     7261 gagcactgct ctgtcgcccc caactctgat accgttgccg aattcgggca agggagggga
     7321 aacccatgaa cgtcggaatc atctgcaccg cgctgcttgg cctgttgttg ttcgggctcg
     7381 gactttatgt ctcgatcctg cgcgggcgca cgaggcgctc catcggttgc gacacgaacc
     7441 caaccgatcc cctgcatcgc gcggtccgtg cccatggcaa caccgcagag tacgcgccgt
     7501 tccttggtct gttgttcctt tggttcgcca tgcatccggc ctcgagttgg gtcgcgggtg
     7561 cgatcgtgat cgcaaccctc gcacgcttcc tgctggttgc cggactgttg tggggttcgt
     7621 cgctcgacaa gccgaacgcg gcacgatttc tcggggcact cctcacctat gtgtgcggac
     7681 tcgcgctggt ggtgcggctg gcggtagcgt aaatcaaggc gcagtgttcc gccgcccctt
     7741 tcccgtgagc tgaatttagt tcgcatatga aatatcgaat tgcagcccga acactgctgc
     7801 aagttgtaaa tgtggcctta agctcgactt gaggcggcga cggctgcgaa gtcccgaggc
     7861 ggcgcttgat cgagcgaccg agacggctcg tccgcgcaaa gccatgcggc aatccgccgc
     7921 cagccgcggt tgagggcggc ctctcccatg aacaggccga gatgaccgca aggctctacg
     7981 gcgatctcga cctgcgcagc cggcgtcccc aggagccctg ccgtcgccag cgcctgctcc
     8041 gcgggaacga cccgatcctc cgtaccgacg agcagaaaca cgggcagcgt gaagcgccgc
     8101 agatcgatcc gccggccaag cgcaacaaaa cctccctttg caatacggtt ctcgcggaag
     8161 acccggtcgg cgacctcgac gtaatacacg ccgggaaggt ccagcgtggc gtcgttccag
     8221 cgttggaacc gggccaggag gtgcccgccg tcattcgcgt cggcaaaact ctgcctcacg
     8281 gcttcatcag gcgcgttcag cgcggccgac cagaccgagc gcaattgcag gccgctcacg
     8341 aggccggtcg tcgcattgac gagccccgcg aaagccgcgt gcggaagcgt ggcggccatt
     8401 tgcgacagcg gcgattggca ggacacgtcg acaggcgcgc ccaccagcac cagccgccgc
     8461 accttctcgg ggaagcgcgc ggcatagacc agcgacagcc agccaccctg gcagagaccg
     8521 acgagatcga ccggtggtcc gatttcgtcg atggcgacat tgagatcggc gagataactg
     8581 tcgatggaga gcgaacgcat ctgtggcgta gccgatcgcc aatcggtgac atggacgcgg
     8641 ctcacgccgc cctgctgcag cgcctgcacg atgctgtgtg caggcgcaaa gtccgtgatc
     8701 aaggcgctgt gcagcgcata gggcgcgcag atcagaacgg gccgcccggc cttttgcgcg
     8761 gagaaatcgc gcagccgcag cgtggtaagt tcgagcgtaa ccctgtgggg catcgtccag
     8821 ggaagagcgg cctcgtcatc gaaaggcggt ttgcgatggt ccccgaacca gcccaaccac
     8881 gcatccagca tcgcttgcgc cccggcgagg ggcaacagga acagatcgcc cgcgcgaatc
     8941 tcttcccttc cgcttgatcc tgccgcctgt ttcggttgcc gcgtcaattg tccaactccg
     9001 tacaacggtt gctgcttcag cctacgccca tcagccattg accctcatca aaactacgca
     9061 accggggtgg cgctacggtg ccgcggcttc tggaacatcc gggagaagaa tatgccgtat
     9121 cgcacaaacg acgatcttcc gccagcggtg cgcaaccacc tgcccgcaca cgcccaggac
     9181 atctatcgcg aggccttcaa ccattccttt acctcgcaca gcggcgatcc cagacaggag
     9241 gagatcgcgc atcgcacagc ctgggccgcc gtcaagcgtc tctacgccaa ggtcggcgac
     9301 aactggatgc cgctgcctgg gcactgagca aatctccgcc ttgcgaagac acacgttccc
     9361 gcaacgcaat ttgcgcccgg atctttccag cacacgtccc tcactgcggc atctgaactg
     9421 attgcccgac cgggcaaatc agtcgcacaa tcgcgtttga tggcggcttc atcccgacat
     9481 ccggaatgac ttgccgacga accaaaggcg acctcagaag ttagcgtcag cctttctctg
     9541 tcgcgccctt tcgacgcaac gccgcagaag tgtccaacca gcgatgcaag agccacgatg
     9601 aagcgattag tcaccgtcaa tgaccacatg caaaagggtt ataaataccg gctcgttgcg
     9661 cccatcggac gtaacttcga tcccgaattc gatccagatc tcacgccgaa ggacatgttg
     9721 cgcctcgggg tgtttggcgg caagtacatg accgattgcc ggaaggagtt tccgcacaac
     9781 tggttcgtgc gagcaaaatt gtcacccaag cgcgccgatc ccgcgctcaa ctttttcggc
     9841 gtgacggcaa gccagcccct cgccgaatgg cgacgcaagg gatggatcca tcctgacgat
     9901 cctcgtggct ggtttcagtg gtattgccgc tattacatgg gcaggcgcat gccgcaggag
     9961 gacgcacggc agatcaagcg gtggaaggcc atacgccgtc atgtgcggca gatacaacag
    10021 cactgcgagc gcggcgatct gctttgccgc agacgccagc gccaggccct gctgcattgg
    10081 gcctatgaca gccggaaact ctagagagcg atcgcgatcg atcggcgcaa atctatttta
    10141 tttttgtttc ggccgcgatc aactctttgc cttccgccgt cctgatcgtc agccgcactt
    10201 gttcgccgtt gccggccgga atcgccgccg aaacacgatt tccgtttttt gggtcggctc
    10261 tcacgtcgtc gtcgaacaat acggcatcgc cgcgcttcaa cgtgacgcgc aacggcgtag
    10321 ccggttcgag actgaagaac tgaagattca cgcgggacgc ctcggcagag agattgacca
    10381 gcatgttgtc gttggcgtgc gtcacgttgc ttatgccgac ggtcgggctg taggtttccg
    10441 gaatcgtgac ttcgccggcc gcgggaaagt cggcgctgtg gaagaattgg tccgacacgc
    10501 ccgcccacaa ttcgacgtag ggctgcgctt cgttcggatc cttgcgtgcg tcggtttggt
    10561 tcgtgaacga cggaaatccc cacgtccaca ttttcaggcc cggcgtgact ttgttgtcgg
    10621 cgatgcggat gattccctct tcgttgtcgt ggttgatcac gccccagaaa ttgccgccct
    10681 gcatatcggg cgccgcgtag gcgatgccca tggtcggcca gttcttgaag taacgcagtt
    10741 tttcgaagcg gcttgtgtcc ccccctgcgc tctcttcgcc gtccgccaga tttttcgacc
    10801 agtggggcgt ttgataggcc ttgatcggcg cgatgatttc ggcgccgccg gtcgctttgg
    10861 gattgttcgg gtccgatccc ggcgcgagcg tcgtacacgt ccagtactcg tatttgatcg
    10921 tccgggcttg cggatttttc agcaccacgc gcgcatcgag cgcggcacga tcggctttca
    10981 gcgtgacata ataggtcgcc tccagtcccg tcgaacccct gaagaactgc cgcggtgccg
    11041 cggaatatgc gaagtcgtcc ttaagcgaca tcgaaaccgt cacttcgccg gcactctcat
    11101 tcaccacctt gaaatcccat ggcttcagcc aggtcttgcc gtgctcggca tccgggaagg
    11161 tgggaaagac gccgccatac accatcagcc agtcgtaata aaaattgccg gcattgatcc
    11221 cgtaaggcac gccgacctcg ttgcggtaaa gttcttcgtg gccggtcggc ttgtaaatga
    11281 tggagagaat gcgcccgcca aattcaggca cgagcgttac ctttaggtaa cgattctcca
    11341 gcacgcgggt cttgaacgtg cgatcgacaa tggtcgtctt gtcgagcgac ccctcgacaa
    11401 aaccgttatc cgtattggtg gcgtatttga cggtgctcca cctgatcgtc gcctcgctga
    11461 gagacacttt gtcggcgact tccgacgccg catatcctgc ggcacgaaac gcgcagacca
    11521 gcgaaaggac tgtaaccgcg aaacgggcac ggttgtgcgc gggagcacga attgccatgg
    11581 cattgcctgg gaccgggcag gagcgacaat tctagtacat cgatgcgcct gcgacgccaa
    11641 accgggtcga cctcatcgga acccaagaca atcaaacaaa aggccccctt ccgggggcct
    11701 tttctttcag actgcgtcct tcgcagcctt taccggtctt gcgcgtacaa ttttgcgcgc
    11761 gggcttggcc ttgaacatca tctcctcgcc cgtgaaggga ttgacgccct tgcgcgcttt
    11821 cgttgccggc ttcttgacaa cgacaaattt cgcgaagccc gggacaagaa atatcccgtt
    11881 cttcttgagt tccttgtgac cgacatcgac cagcatctcc atgacgtttt tcacgtcgcg
    11941 tttggacaat tcagtcgacg tagcaattct ctcaattagc tgcgacttgg aaagttgcgt
    12001 aggcattgcc cctccttcga ttcgaaccaa ggagacaata aacgagcgag aaggcggcaa
    12061 aaaccgttct gcacagattt attggctcgt gacgggcctc cacgggcgct tacggttggg
    12121 ccggttgcgt ccgcggcggc gacagagcgg cgctttccga aaaataaaaa agccgccctc
    12181 tcaagcggct ttcctagatt gaagccgcgc cagcggcaac ttgtttggtt gcggggcgcg
    12241 cgtaacggac acctccccaa actgcctcaa actgttattt ccatgcaatc acaaggggtt
    12301 aggcgacctg atccccaggg cttgccgctc aaactccgat cgccaatttt ggcaagatcc
    12361 tgccgggtat ggagccagac ggcgcctggc ccaggtcgta agctcccatt cgtcgataga
    12421 atggagccgc accgggatct gaatcgatga ttagtcgggt ggcgcccctt ttttttgcga
    12481 catgggtagc ccaggcaaac agcgccctgc ctacgccgcg atggagagcg ctgggttcga
    12541 cgaacagctt cagcaaatca gcctcatcgc ccgtcacttt cacttgcgca agaccaacca
    12601 ccttaccgcc aagctcggca actgcgatcg tcgtcaactg caagtcccgt tggtcaaacg
    12661 ataactcgct gcgacacgcc tccagaaatt cccggttgta gccccaggtg gcttttgacc
    12721 tgagacacag ttctgagagg ctgggtagct cttcaatcgt gggggcccgg atcatcggct
    12781 gcaaactaac cccggatcat ctgagaagag tttatcacag atcatcagac gcctctcgtc
    12841 ggacagaccc ggaagttcgg tccagcacaa tgtccgcact accttttccc gaaagcggac
    12901 gtcagaatga cgcacctcat gttgctaggg gttaaaggga gaaatccccc gactattcaa
    12961 tcaccttgtc cgcactcgcc agcagcgcgg cgggaatatc gagctcgatt gctcttgcgg
    13021 ccttcaggtt gacggcgaac tgaaatcgtg tgggctgcag ataaggcaga tcgccggggt
    13081 tcgcacctct gaggatcaag tccaagtaat gcgccaaccc catcaagact tcgctgaagt
    13141 cgctcgtgta gaccacgttc tgtcccggcc agtggttccc aagttgctcg ccgatgtaac
    13201 gttatggatt gtgccgtcgc cgccatagcc gagcgtctcg gcacatgcac gatcgtctat
    13261 cggcaacacc agatcatcaa aattgcggaa aatatcattt cttgcggtga aaaagaattc
    13321 aaccgttgat tcctgcagcg ggaatgtaaa acctagatcg atccgaaggc gcttgaagca
    13381 acggaaaaca aacaaacgca gcgcgtcagg agagcatttg tctggaatcg tctacgtcgt
    13441 tgatgacgac gcattcttcc gtgccgccat ccagcagcaa ctggagcaga ctggttaccg
    13501 tgtggtcacc tatgcatcgg ccgaggaggt gctggagcaa cggcccgatg aaaatgggcc
    13561 cggctgcatt ctcctcgagg ttcggatgcc cgggttgagt ggaccggcgc tccaaagccg
    13621 cttgctggag ctcggctcaa ctcttcccat cttgtttatc acgggctacg ccgatgtgag
    13681 cacgaccgtg aaggcgctta aggcaggcgc cgacgacttt ttgattaagc ccgtgcaatc
    13741 ggatgagttg ctgcgggcaa ttcggcgatc tctggcgcgt caccagaccg cacttgaggt
    13801 aaagcgcgaa ttcgatgttc tgcgaggccg cctcgcaact ttgaccccgc gcgaacgcca
    13861 ggtatttttg ctcattgttc ggggcaagat caacaagcag gtagctaatg agttgggggc
    13921 gaccgaacgg accatcaaag cgcatcgcca caacgtcatg gagaaaatga aggttcgaag
    13981 cctggctgag cttgtgctga tcggcgaacg gctgggcgtt tccgcgcgtg actgattatg
    14041 gcggatgcaa ctttacggaa atctcgaacc agtttcccgc cggaatgcgg ttggcgtaac
    14101 acccacaaat ttccgaaaca cccggttcag ttgggatgca tcgctgaacc ctgccgctag
    14161 cgccacgtcc ccaagcgaga gatcgggttt ggacaagagt cctctggcac ggcggatccg
    14221 ctccgacagg acgtactggt aaggcgtgag cccaactgtg tccttgaacg acctgatgaa
    14281 gtggtagtgg ctcagtcctg ccagggccgc caagtcggaa atcgtaatct cattcgggat
    14341 ttgagcttcg atgaattcct tcacgcgcct taactgacgt gcagtcaaac ctccccgaac
    14401 cgggttcaga cgcaaatgcc ctgggtcagc ggcgtgccgc agttcccaca agagcaatag
    14461 cccaagtgtc tccgcatacg cacggtcgtt tatgccggag ccatccagga cactttggag
    14521 cttttgcagc gtcgctctga gattgttgtt ttcgaaatac agcgctgagg gaatttttga
    14581 aatatcgttt gcgtcatgat ctgacctcgt tgaggtgaga tgaactgcaa agacggacga
    14641 cgcgcggcct tcgaagcgat tccagcctcc taccgaagat ccgattggca cgaacgtcaa
    14701 cttatctcgg aggtcagtca gcgtcgaacg gctccctccg tctatagcgg tctcgccatc
    14761 ggagcgaatg gaatcatgca gcgccagata ggctgcatct cccttcaccc gaaattccaa
    14821 ttccgtccgc caaacgcgaa caagctcggc agagaaccaa gaccacgaca tccgcttgat
    14881 tacgaatggg cgccgcgcac cgggttgtcg cggatgccac tcgagcttac cgaacgggtg
    14941 caagcgcttc ctccaaggat acctgtctgt ctcagggata ctgcaaatag ctgcggaatc
    15001 cgcaacctta cagtcatcac ttgcaccaaa tagctcttct cccgcaagag gaactccgcc
    15061 actgatctct tgccgcgcca caagagcgcg gcttcgggcg ctcaggagag ccgaaatacc
    15121 cggagatccg gcattcgcgt cgcggtgcga ttgccctgcc gatcactagt caattgcgaa
    15181 agtccgaccc cgcgcggtcg agtttgcgca gaagcgctgg ccagaccagg gtgtcggcaa
    15241 gcgatgacaa gccgccccct cgcgtcaatt ccgcgttctt ttcgatcact gcttcgtcgg
    15301 tcgggtgatc catcgagaca tcaccaagca tgcgcgtgcg gacctgcatc gtgcatgcgc
    15361 gttcgagaag atacatccgc aggaatgctt ccgcgcagtt cttgccgacg gccagcgtcc
    15421 catggttgtg aaggagcatg aggttctttt gaccaagatc ccgctgcagg cgcggccgtt
    15481 cgtcataatc aagcgcaacg ccttcatagt cgtggtaggc gagatcggga aggacgagtt
    15541 gcgcagtctg gttcaacggt agcaaacgct ctttcgatgt cgagacagcc gtgccgtccg
    15601 gcgtatgaag gtgcatgacg cagcccgcat cttcacgaac ttcatggacg gccgaatgga
    15661 tcgtgaaccc ggctgggttg actggatagt tgctcttgcc gaccgggtta ccgtcgagcc
    15721 cgatcttgac gaggctggag gccgtgattt cctcgaacat caggccgtag gggttgatca
    15781 ggaagtgatg ttccggtcca ggcacacgcg cggagatgtg ggtgaaaata agatcgtccc
    15841 agccatagag cgcgaccagg cgatagcagg cggcaaggtc gacgcgaatc gcatgctcgt
    15901 ctggaggaag tacgttttcg ctcattcttc gctctcccat ttgttgcgtg tttcggtaat
    15961 ctttagccat gagcccggat aatcttatag ccgtgggcta tggcggtcga gcaggcatgg
    16021 gcgctcttcc gtgatcttgg cgaggcaacg tcgcctttag gggaaagagc gccgcgccgt
    16081 tcttcttgat ctaaatcaac gatgtctgac ttgggtaatt tgcagacatc gcaaggtgat
    16141 cttggcggat atccgctcta ccccgaaagg agacctcatg gtcacgtacc ggcatgtccg
    16201 ccaagggcca ctttcacggc agctttgatc tatctcaagt ggtctaacca gttaagccgt
    16261 tggggtttga ctacagggac taagtttcga cgactatctg cgcgaagttg atccatgagg
    16321 agatggagac ctgaccgtct ggaggtggcg aggtgggcac tgtgcgtgct catcgccgcg
    16381 ctcgctgtcg cgaacgccgc gtttgaaaaa gcttgggcac gcgacacggg aagtctttcg
    16441 cgagcggacc ttcggtcggt cagcgacttc atgcgtcatg aggtcgcgac cgggcagatc
    16501 gcgggcgcga tcgtgctgat ccagcagcat ggacatcccg tctatttcga aaaattcggc
    16561 ttgcgcgaca ttgggcccaa gctgccgatc acggacgata ccatctttcg gctctactcg
    16621 atgtcgaagc ctgttacgtc ggtggcagcg atgatgctgg ttgatgacgg caagctatcg
    16681 ctcgatgatc ccgtcgccaa atatattcca gcctttgcac atctggaggt cggtgtcgaa
    16741 aggcacgaca ggactggaaa ggcggtgctt acgcgcgagc cgctgaagcg tccgatcacc
    16801 atactggatt tgctgcggca cacctccggt cttacttacg gattttacgg tgagagcgcg
    16861 gtccgaaagc tttacgcgaa agccgacctc tacactggcg atttcgacaa cgccgagttt
    16921 gtcacgcgcc tggcaaagct gccgctcgcc gagcagcccg ggacggtgtg ggactacggc
    16981 gtttccaccg atgtgctcgg cagggtgatc gaggtggttt cggggcaatc gctgtttcag
    17041 ttcgagaagg agcggttgct ggatccgttg ggcatgactc acacggcgtt ttacgttacg
    17101 gacgaggcca agcgcgagtt gatcgcccaa ttcctcccga aagaaacctg gaaggccttt
    17161 atcggaatca gggagccgat ggagcgcacg ccctgggaat ccggtggcgc gggcctgaca
    17221 ggaacggtcg gcgattatgc gcgctttgcg caaatgctgt tgaacggcgg cacgcttgac
    17281 ggccggcgct accttaagcc ggagacggtg gcccggatga cgtcggacaa tatcggcccc
    17341 gggggcggaa tcgcacacac ccatctctat tttctgagta ccgagagtgg attcggattg
    17401 ggctttgccg tgctaacggc gaagccgcga aacaagccgt ggccgccggg cgaatatggc
    17461 tgggccgggg cggcgggcac ttttttcttc gtcgatccga aaaacgacct gttcgtgctc
    17521 tgcatgatgc agacacattt gcaccgcgga cggatcgagg gagaattggc gaggctcgtt
    17581 tatgaggcga cggcgaggca ggccgtctcg agccgctagg cccggattcc acaatcagtt
    17641 cgagcgttgc ggagatgttg gcagcgatca cctcaaactc tccgcctgac gaagcgtttg
    17701 gaaccggcgg ccaactcgcc gccctgctca gggtttccga gaggacggcc gagcccctgg
    17761 caaagtccag gga
//
LOCUS       DATLJT010000854         6427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_972726,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000854 DATLJT010000000
VERSION     DATLJT010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972726"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VM910_19570"
     CDS             <1..706
                     /locus_tag="VM910_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI64761.1"
                     /translation="LRCLEIQPVWRYPTASQLAFDLGHPDQVKLTARSEKIKRDPLST
                     VWRRRFNQGVTQPKPKSDMAAQLAASPILAVAVDIAEGSEEINDAVRRSAERVMATLP
                     SARLACLNVLKLGRITIDRTLDEQGHNKHVDRLVALRHWASPFKLDEQRLTVHVLEAV
                     DPASAILEFATVNRVDHIIVGARRDSMLRSLLGSVSSRIAAEASCSVTVVRPPRSIPP
                     PAPADASQGQDMPPVF"
     gene            complement(732..1433)
                     /gene="rpe"
                     /locus_tag="VM910_19575"
     CDS             complement(732..1433)
                     /gene="rpe"
                     /locus_tag="VM910_19575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVI64762.1"
                     /translation="MANSFTPRPLVIAPSILASDFAKLGDEVRAVDQAGADWIHLDVM
                     DGHFVPNISYGPDVIRALRPHTKKIFDAHLMIAPCDPYLEAFAKAGCDHITVHAEAGP
                     HLHRSLQAIRALGKKAGVSLNPSTPISAIEYVIDMVDLVLIMSVNPGFGGQAFIPSAL
                     GKISDLRAMTAGRDIDIEVDGGVAPDVAGPLAAAGANAFVAGSAVFKGGTVEAYRTNI
                     SAIRNAAALARGEAI"
     gene            1700..2509
                     /locus_tag="VM910_19580"
     CDS             1700..2509
                     /locus_tag="VM910_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HVI64763.1"
                     /translation="MLLALGAVSSVWDALQSLTSSSNASSAPSANSGQTTTDPFDIGG
                     ATSTPASGSSFFGAGGGPQISPQTMSALLAAQSQAGSTGASPLDPSQALQDLFSQIDA
                     GSNGGISKSEFESALGAGGTNTAAADDVFSKLDTNGDGSVSFDELKSALEGAGHHGHH
                     HMHAGGVGASGDCDGADGATASSAGTDPLAQALAGASSTSVTNGDGSTTTSITYADGS
                     KVTLTTPAASTTSTSLAPSSATSSYNLIEQLIQREAQALSASVASSLSVSA"
     gene            complement(2527..3522)
                     /locus_tag="VM910_19585"
     CDS             complement(2527..3522)
                     /locus_tag="VM910_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HVI64764.1"
                     /translation="MKNLITDIAGLRVGHADDARLASGVTAIVFDKPAVAAIDVRGGG
                     PGTREDAALGLANTVDRIDGIALSGGSAFGLEAGGGVQAYLAERGRGFVVRDAVIPIV
                     PGAICFDLLNGGDKAWGRFPPYRDLGYRAAAGAAVDFALGSVGAGLGASTATFKGGLG
                     SASAKTPAGVTVGALVVVNAVGSVTIGDGPWFWAAPFEQDNEFGGRGLPPSFSPDMLK
                     LRIKGGPTATEAEATTLAVVATDAALTKPQAKRLAMIAQTGMARAIYPVHAPLDGDLL
                     FAVATGEKPVDPLVGLTELGMVAANALARAIARGVFHATALSFPGALPAWKDRFG"
     gene            complement(4437..5201)
                     /locus_tag="VM910_19590"
     CDS             complement(4437..5201)
                     /locus_tag="VM910_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64765.1"
                     /translation="MECPFCAETIKDEAIACKHCSRDLRVVRPMLREIDDLVAELDKL
                     RRDLDRVNVTLERYKNPLRYFGSHVILYVLVPSLLLVIAHILVTIVFDLSPLPLRLAS
                     VAIPILFGFIAYPLHKVSMNGALALAFATAALSVLAMLTVTGLHDHVPILPEVWVEWR
                     EVIEYGASILLAFVSGNLLGILIFQVMPRVLQQGGKPNALAFRIARLLGQHVGEEQLR
                     RRARLIQNLIQTAGPLIGVAATAVGSIYAGLKGILG"
     gene            complement(5205..5402)
                     /locus_tag="VM910_19595"
     CDS             complement(5205..5402)
                     /locus_tag="VM910_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64766.1"
                     /translation="MNCPYCRSENADDALVCATCSRDIAVPPTLIAERDELLRKRDLL
                     LDELRRARSEIEMMKSRRTSR"
     gene            complement(5410..5850)
                     /locus_tag="VM910_19600"
     CDS             complement(5410..5850)
                     /locus_tag="VM910_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HVI64767.1"
                     /translation="MMKRTWLGLIAAGVLVSGAAQATQLITDEEAKLPPPKGAIAADR
                     RGILRGPRVEVVSPSGVVHSPLRLQLKFESFGGATINPDSVKVLYLRAPNVDLTPRVK
                     PFIQSGGIDMPDAELPPGEYTVRVDVKDSDGRPGTVSFTLTVSP"
     gene            complement(5881..>6427)
                     /locus_tag="VM910_19605"
     CDS             complement(5881..>6427)
                     /locus_tag="VM910_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HVI64768.1"
                     /translation="GKNASEGAVKTMNLSGFRILAFATHGLVPGELNGLTQPALALSS
                     PTVTGEEGDGLLTMEEILGLKLDADWVILSACNTGAGAGAGAEAASGLGRAFFYAGTR
                     ALLVTNWSVHSQSARQLVTDLFKRQADDPKIGRSEALRQAMMALVDGPGYLNGEGKTE
                     FTYAHPLFWAPYTIIGDGGVR"
BASE COUNT         1182 a         2138 c         1995 g         1112 t
ORIGIN      
        1 gctgcgatgc ctcgagatcc agccggtctg gcgctacccg accgcgtcgc agcttgcctt
       61 cgacctcggc catcccgacc aggtcaagct gacggcgcgc tcggagaaga tcaagcgcga
      121 tccgctctcg acggtatggc gacgtcgctt caaccagggc gtgacgcagc cgaagcccaa
      181 atccgacatg gcggcgcagc ttgcagccag cccgattctc gcggtcgcgg tcgatatcgc
      241 cgaaggatcg gaggagatca acgacgcggt gcgccggtcc gccgagcgcg ttatggcgac
      301 gctgccatcg gcgcggctcg cctgcctcaa tgtcctcaag ctcggccgca tcaccatcga
      361 ccgcaccctc gacgagcagg gccataacaa gcatgtggac cggctggtgg cgctcaggca
      421 ctgggcctcg ccgttcaaac tcgacgaaca gcgcctcacc gtgcacgtgc tcgaggcggt
      481 cgatccggcg tccgcgatcc tcgaattcgc gacagtcaac cgcgtcgacc atatcatcgt
      541 cggcgcgcgt cgggattcga tgctgcgctc gctgctcggc agcgtctcct ccaggatcgc
      601 tgcggaagcc tcttgctcgg taaccgtggt gcggcccccg cgttccatcc cgccgccagc
      661 gcctgcggac gcttcgcagg gtcaggacat gccgccggtt ttttgaccgg ccgaaaggca
      721 cctgagcccg cctagatcgc ttcgccccgc gccagcgccg cggcattgcg gatcgccgag
      781 atatttgtcc tgtaggcctc aaccgtgccg cccttgaaca cggcggagcc cgcgacaaag
      841 gcgttggcgc cggcagcggc aagcgggcca gctacgtcag gcgcgacgcc gccgtcgacc
      901 tcgatgtcga tatcgcgccc cgcagtcatc gcgcgcaaat cgctgatctt gccgagcgcg
      961 gaggggatga aggcctggcc gccgaagccg ggattgaccg acatgatcag cacgagatcg
     1021 accatgtcga tgacatattc gatggccgag atcggcgtcg acggattgag cgagacgccg
     1081 gccttcttgc cgagcgccct gatcgcctgc aacgagcgat gcaaatgcgg acccgcttcc
     1141 gcgtgtacgg tgatgtgatc gcagccggcc ttcgcaaacg cctccagata gggatcgcat
     1201 ggcgcgatca tcaggtgcgc gtcaaagatc ttcttcgtgt gcggccgcag cgccctgatg
     1261 acgtcagggc cgtaggagat gttcggcacg aaatgcccgt ccatcacgtc gagatggatc
     1321 cagtcggcgc ccgcttggtc gacagcgcgg acctcgtcgc cgagtttggc aaaatccgac
     1381 gccaggatcg atggcgcgat gacgagaggg cggggcgtga acgaattggc catgggtgat
     1441 ttccgcaagg acgcccccgc ctaacacggc cgcggggccc gagcaatgcg ctccgccgcg
     1501 gcaacgcctg ctgctttcgc cgggttgcgc gggagggcag gaaaaatctg ccgtcgcggc
     1561 atctattgcc ggggtttgcg taccgtggct ctgaccgaaa gtccttgttc catgcggctt
     1621 ttcaccgatg gcacggcgct tgctcggcct ttgcgcgagt cgtgccggcc gtctcgcgcg
     1681 gcccttgcgg agtttgatca tgttgcttgc cctcggcgcc gtgtcgtcgg tttgggacgc
     1741 cttgcagtca ctgacctcgt cttcgaatgc ctcgtccgcg ccatccgcca actccggcca
     1801 gaccacgacg gatcccttcg atatcggcgg cgccacgagt acgccggcga gcggctcctc
     1861 cttcttcggc gcgggcggcg gtccgcagat atcgccgcag acaatgagtg cgctgcttgc
     1921 ggctcaaagc caggcgggtt caaccggcgc gtcgccgctc gatccctcgc aggcgctgca
     1981 ggacctgttc tcgcaaatcg acgccggtag caacggcggg atctccaagt cagaattcga
     2041 gagcgcgctc ggcgccggcg gcaccaatac cgctgcggcc gacgatgtat tctccaagct
     2101 cgacaccaac ggcgacggca gcgtcagctt cgacgagttg aaatcggcgc tcgagggcgc
     2161 aggccatcac ggtcaccacc acatgcatgc gggcggcgtc ggggcgagcg gcgattgcga
     2221 tggcgccgac ggcgccaccg cgagctcggc gggtaccgat ccactcgccc aggcgcttgc
     2281 cggtgcgtcc tcgacctcgg tcaccaacgg cgacggctcg accaccacct ccatcaccta
     2341 tgcggacgga tcgaaggtca cgctgacgac gccggctgcc tcgacaacgt cgacatcgtt
     2401 agcaccgagc tccgcgacct cgtcctacaa tctgatcgag cagctaatcc agcgcgaggc
     2461 gcaggccctg tccgcttcgg tcgcgtcatc gctatcggtc agcgcctgag ccatatcccg
     2521 cgtcggtcag ccgaaacgat ccttccacgc cggtaacgca cccgggaacg acagcgcggt
     2581 cgcatgaaac acgccgcgcg cgatcgcgcg cgcgagcgcg ttcgccgcaa ccatgccgag
     2641 ctcggtcagg ccgaccaggg gatcgaccgg cttctctccg gttgcgaccg caaacagcag
     2701 gtcaccgtcg agcggcgcat gcaccggata gatcgcgcgg gccatgccgg tctgcgcgat
     2761 catcgccaat cgcttggcct gcggttttgt cagtgccgca tcggttgcga ccaccgcaag
     2821 cgtggtggcc tccgcctccg ttgcggtagg gccgcccttg atccgcaact tgagcatgtc
     2881 gggcgagaac gagggtggca gccctcgacc gccaaattcg ttgtcctgtt cgaacggcgc
     2941 tgcccagaac catggaccgt cgccgatggt cacgctaccg accgcgttca ccaccacgag
     3001 cgcgccgacc gtgacgccgg cgggcgtctt cgccgatgcc gatcccagcc cgcccttgaa
     3061 ggtcgcggtc gaggcaccga ggccagcgcc gacgctgcct agcgcgaagt cgacggccgc
     3121 gccggccgca gcacgatagc cgaggtcgcg gtacggcggg aatcgacccc acgccttgtc
     3181 gccgccgttg aggagatcaa agcagatcgc cccgggcacg atcgggatga cggcgtcgcg
     3241 cacgacgaag ccgcggcccc gttcggcgag gtaggcctgc acgccaccgc cggcctcaag
     3301 cccgaatgcc gatccgccgg atagagctat gccgtcgatg cgatccaccg tgttggcgag
     3361 accgagcgct gcgtcctccc gcgtgccggg tccgccgccg cgtacgtcga tggcggcgac
     3421 cgccggcttg tcgaagacga tggcggtgac gccggaggca agccgcgcat cgtcggcatg
     3481 gccgacgcga aggccggcga tatcggtgat caggtttttc aacgaacgga cccacagggt
     3541 tgatcgacga ccacgcatat atcagccgcg acgcaagctt caaactgtcg cggcacaact
     3601 tcctattcag ttgtcaaaca gccgggcccg tcattgcgag ccgacggggg acgcgcatcc
     3661 gcattcccgc ggcgcgatgg cgtccgggtt ttgcgtcgat tttgtccctc gaaacaacag
     3721 agggcgcagg gaatgccggg tgttttgctg cacccgcagc cttgtgtgcg aatggaagaa
     3781 gcacacaagc gtagtcacca caggtccagc cgaaacatcg acattccctg cgcgatggtt
     3841 ttaacagttt ccttcgagct ctccccggag accaggcatt gttgcctccg tcgcctgcag
     3901 tcatcacctg ccgacttgat gccagccttg gggcacagct agcgcccgct ttccgaagtt
     3961 cgtgcgaacc cgcagcatcc acgttcacga acttcgaaaa gcaaagggcg ctagcaaaat
     4021 caatatttcc agtgccgctt tcgatttgaa gttccagttg cggactcgcg gcgcctctgc
     4081 aggaacttca aatcggcggc actggcacca cacgacttcg ccgtccgcgc tcatttcgct
     4141 cgtctggcgg aacaatcgcg tccatcgcat cccgcttcca acactcgtga cgatcgcgaa
     4201 gcgcccctct cgtggaggcg ggacggcacg aagatatgca taagttctga ttttcggaaa
     4261 gagaaatttt ttcatgtacg ggtatgcgca ccctattcat gttgaatcgg ctgatgaaac
     4321 tcgttttttt gcgcaggcgg tttggcagct tggaggcgtt cgcgagaatg gcaacaggcg
     4381 ccaggaaatc gagctgattc gcctggtcgg gccaatcagc gcgacaagca gggctgtcag
     4441 cccagaatgc ctttcagacc ggcgtagatg gagccaaccg cagtggcggc gacgccgata
     4501 agggggcccg cggtctggat caggttttga atcagacgcg ccctgcgtcg cagttgctcc
     4561 tcgccgacat gctggcccaa taaccgcgcg atcctgaagg cgagagcgtt gggcttgccg
     4621 ccttgttgca gaacacgtgg catcacctgg aaaatcagga tgccgagaag gttgccggag
     4681 acaaaggcca ggaggatgct cgcgccatat tcgatcacct cgcgccattc gacccagacc
     4741 tccggcagga tcgggacgtg atcgtgcaag cccgtcaccg tcagcatcgc cagaacgctc
     4801 aaggctgccg tggcaaacgc cagcgcaagc gcgccgttca tcgagacctt gtgcagggga
     4861 taggcgatga agccgaacag gatcgggatc gcgaccgatg ccagccgcag cggaagcggc
     4921 gacaaatcga agacaatggt gacaaggatg tgcgcgatga cgagcagcaa cgagggaacg
     4981 agcacataaa ggatgacatg cgagccaaaa tatcggagcg gattcttgta gcgctccagc
     5041 gtgacgttga cccgatcgag atcccgccta agcttgtcaa gttcggcgac gagatcgtcg
     5101 atttcgcgca gcatgggccg caccacgcgc agatcgcgcg agcaatgctt gcaggcgatc
     5161 gcttcgtcct tgatcgtctc ggcgcaaaaa gggcattcca ttttttaacg cgacgtcctg
     5221 cggcttttca tcatctcgat ctcgcttcgt gcgcggcgca attcgtcaag cagcagatcg
     5281 cgcttgcgaa gcagctcgtc gcgctcggcg atcagggtcg gcggtacggc gatgtcgcgc
     5341 gagcaggtcg cgcagacaag cgcgtcgtcg gcattttcgg atcggcaata ggggcaattc
     5401 atcgggcgat catggcgaca ccgtcaaggt gaagctcacc gtgccaggac ggccatcgct
     5461 gtccttcacg tcgacccgca cggtgtattc gcccggcggc agttcggcgt ccggcatgtc
     5521 gattcctccg gattgaatga acggcttgac gcgcggggtc aggtcgacgt tcggcgcgcg
     5581 cagatagagc accttcaccg aatccggatt gatcgtggcg cctccgaagg attcgaactt
     5641 caactggagc cgcaggggcg aatgaaccac cccgctcggc gagacgacct ccaccctggg
     5701 tccccgtaaa ataccgcgcc ggtccgcggc aatcgcgccc ttcggcggcg gcagcttggc
     5761 ttcctcatcc gtgatcaact gcgtcgcctg cgccgctccc gagaccaaaa ccccggccgc
     5821 aatcaagccg agccacgtcc tcttcatcat gcgtttgccc aaaaccttca cgttccccga
     5881 ttaccgcacg ccgccatcgc cgatgatcgt gtagggtgcc cagaacagcg gatgcgcata
     5941 ggtgaactcg gtcttgcctt cgccgttaag ataaccgggc ccgtccacga gcgccatcat
     6001 cgcctgtcgc agcgcctcgc tgcgtccaat cttcggatcg tccgcctggc gcttgaacag
     6061 gtcagtgaca agctgccgcg ccgattgcga atgcaccgac cagttcgtca ccagcaacgc
     6121 gcgtgttccc gcatagaaaa acgcgcggcc aagtcccgaa gcggcctcgg caccggctcc
     6181 tgcgccggcg ccggtgttgc aggccgacag gatgacccag tcggcatcga gcttcagacc
     6241 caggatctcc tccatggtca acagcccatc gccctcttcg ccggttacgg tcggcgacga
     6301 cagcgccagc gccggctggg tcagaccgtt gagctcgccg ggcacgaggc cgtgggtggc
     6361 aaacgccaga atcctgaagc cggacaggtt catggtcttg accgcaccct cgctcgcatt
     6421 cttgccg
//
LOCUS       DATLJT010000855         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3272058,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000855 DATLJT010000000
VERSION     DATLJT010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272058"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..677)
                     /locus_tag="VM910_19610"
     CDS             complement(186..677)
                     /locus_tag="VM910_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64769.1"
                     /translation="MFLAAAAAFVITSAGLAEQDPDQVNVQGGWAYTAKDKDGAVEHV
                     AATRAAEDAVWLTLGCKADRRLAVSFIHSEHFPFPLKPVSMVKLQSNNVPTALVEAKN
                     VENNQIFVAPLLMRHIMPLIMEDDELIVSIPDRDGAMHSYTFSMQPNDLALKPIRARC
                     FYF"
     gene            complement(750..1505)
                     /locus_tag="VM910_19615"
     CDS             complement(750..1505)
                     /locus_tag="VM910_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI64770.1"
                     /translation="MDGTILNVAIYLTATFAAALVTGVAGFAFGLVAAGVWLHILTPM
                     ETATLIIAFGLVVQGISVWKLRHALRWSRLWPFLLGAAFGVPLGVAILGWARPDYVRV
                     TVGVVLALYSAYSLARPAMKPVKAGGATADASVGFLNGILGGLTGLAGILTTIWCGLR
                     GWPKDEQRAIFQPIGVAIFAMSGLWLGISGAVSTDTVWFFAIGLPALLAGTWLGLKLY
                     GHLDEAGFRKLVLVLLLVSGLALVAPALVPLFG"
     gene            complement(1667..2368)
                     /locus_tag="VM910_19620"
     CDS             complement(1667..2368)
                     /locus_tag="VM910_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64771.1"
                     /translation="MSPLVVMRCVTKSYGRGATAIKALDNVSLEAHLGELVQLTGPSG
                     AGKTTLLNLVSALDRPDMGEILVAGANVTGLSLARAAKYRNNQVGIIFQSYNLLPQLT
                     ALENVLLPMIPNGRMDRDRAFELLDAVGLVDRSAHRPAQLSGGEQQRVAIARALVNGP
                     RLVLADEPTGNLDDENARNVMALLCRTCRNRGSTLIVVTHDRDVVHIVDTVFELRGGI
                     IARAGRATCRNGPIL"
     gene            complement(2371..3630)
                     /locus_tag="VM910_19625"
     CDS             complement(2371..3630)
                     /locus_tag="VM910_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64772.1"
                     /translation="MIWTKIIFGGLGRRGFEAVVAGLVLAAASALVAGSLMVVEGAQY
                     ALMQAERNDRPEIVQIRSRFNRALFETPRSGNLPPLTLPVYEPLIEPEQLSSAAGDGV
                     IIPRQSLFRNVVSGDRFLNVYIFGIDPVAEGQVSSFSLTRGRFLRSDDGVVAVLDEVS
                     ASALGVDLGKTFPVRKADGEDLDLTVVGIIGKLELRDAPPRTVEAPALTQNSSFVSSG
                     VFVTLRTSEAIFGRTTLTDALVVARTPTDVPSLADSLREAFRLEPGVFVTERYSQFSR
                     KVHDFVLTLSLFTLIGTATALFAGVFAANLLHDVYADRRCHYAILIALGVPPARVALV
                     GLSLGLAVAFAGTITAVLMAVFFRPTEFALPSLMANLGAIEPRFDLSVAAAMAAIALA
                     AVALGMTPSWWRLLRHPMATALSENGR"
     gene            complement(3627..>4119)
                     /locus_tag="VM910_19630"
     CDS             complement(3627..>4119)
                     /locus_tag="VM910_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64773.1"
                     /translation="TAVKSLLKERQLTEDCEGSTPRSFCLRLPERNNFQAAFQEQSKV
                     GAGGSFFIALLIALVAVGSAGLQVQTVVTRWREFGVLQAIGFSPGQIVGYYGVQLCLL
                     LGGGIAFAVIASIAVTSSLGSSLASVGLAATVSVVVGGLAALSVLLWPLSRSPAELIG
                     DQA"
BASE COUNT          918 a         1253 c         1185 g          763 t
ORIGIN      
        1 cgttgcaaac aatctgcgcg acttccttca ttcgcaggat tcatcgagtc ccgcggcttg
       61 ggcatttgtt ggctcggatg ttgaaccagt ctgtttaaga cacttttatc aagtgcgcgg
      121 cgcagaccgt gcgagaggcg acgccgacgc agagcgaagg aaacacaggt cggagttttc
      181 gttcgtcaaa aataaaaaca gcgcgcgcga attggtttaa gcgcaaggtc gtttggctgc
      241 atcgaaaacg tataactgtg catcgctcca tcccggtcag ggatcgagac gataagctcg
      301 tcatcttcca tgattaacgg catgatgtgc cgcattaata gcggcgcgac aaagatctgg
      361 ttattttcga cgtttttcgc ttcaacgagt gcagttggta cattgtttga ttgtagcttt
      421 accatggaga caggcttcag cgggaacggg aagtgttcac tatggatgaa cgagaccgct
      481 agccgtcgat cagccttgca tccgagcgta agccaaacag catcctcggc cgcacgggtc
      541 gcagccacgt gctccaccgc gccgtctttg tcctttgcgg tatatgccca gccaccttgc
      601 acatttactt gatctgggtc ctgctccgcc aaccctgcgc tggtgatgac gaatgccgct
      661 gcagcagcca aaaacaaggc aacggcacgt ttcgacctaa tagatcttgc tctctcagat
      721 ttgttgcttg tcctcgagct catcggcgat cagccaaaaa gtggtacgag agcgggcgcc
      781 accagcgcca gtcccgagac cagcaacagg accagcacca gcttgcgaaa gccggcttca
      841 tcgagatgtc cgtaaagctt tagtcccagc cacgtcccgg ccagcaatgc cggaaggccg
      901 atagcaaaga accagacggt gtcggtcgat accgcgccgc tgatccccag ccacagcccg
      961 ctcatggcga atatcgcgac cccgatcggc tggaagatcg cccgctgctc gtccttgggc
     1021 caaccacgaa gaccgcacca gatggtcgtg agaatgccgg cgaggccggt aaggccgcca
     1081 agaatgccgt tcagaaagcc tacgctcgca tcggcggtgg caccgccagc cttgaccggc
     1141 ttcatcgccg gtcgggcgag gctatacgcg ctgtagagcg cgagcaccac gccgacggtg
     1201 acgcggacgt agtcaggacg cgcccatccg agaatcgcca cccccaaagg gacgccgaag
     1261 gccgctccca atagaaatgg ccacagccgg ctccaccgca gtgcgtgccg caatttccag
     1321 accgagatgc cctgcaccac cagaccgaag gcgatgatca acgttgcggt ctccattggc
     1381 gtgaggatgt gcagccaaac gccggcggca actaatccga atgcaaaccc cgctaccccg
     1441 gtgacaagcg cggcggcaaa cgtcgccgtc agataaatgg caacgtttaa gatcgttccg
     1501 tccatcgatc gctcgtcctc ctctgcgcgc gtggcggcct cggcatcgac ggcgtcgcat
     1561 cctgctttcg acgtgcatcc atttgctacg cggcagcaac gcgacggtcg catcggcaaa
     1621 aagccatcga tgcgacttac gctgggagca tttgtttaag gcagttttaa aggataggcc
     1681 cgttgcggca cgttgcgcgc cctgccctcg cgataatgcc gcctcgaagt tcgaaaaccg
     1741 tatcaactat gtgcacgacg tcgcggtcgt gcgtcacaac aatgagagtg ctgccccggt
     1801 tccggcatgt gcggcataac aaagccatta cgtttcgtgc gttctcgtcg tccagatttc
     1861 cggtcggctc gtcggcgaga acaagacgtg ggccgttcac cagggctcgg gcaatcgcta
     1921 cgcgctgctg ttcaccgccg ctcaactgtg ccggcctgtg cgcacttcga tcgacaagtc
     1981 cgacagcatc gaggagttcg aacgcacggt cgcgatccat tcgcccgttc gggatcattg
     2041 ggagtaggac gttttcgaga gcggtcagct gcggaaggag attataggat tggaaaatga
     2101 tcccgacctg gttgttgcga tatttggcgg cccttgcaag cgacagaccc gtgacattcg
     2161 ctcccgcgac aagaatctcg cccatgtcag ggcggtcgag cgcggaaacg agattgagaa
     2221 gggtcgtttt gccggcgccc gacgggccgg tcagctggac caattcgccc agatgagcct
     2281 cgagcgacac attatcgaga gctttgatgg ccgttgcccc tcggccgtag ctcttcgtaa
     2341 cgcatcgcat gacgacgagc ggcgacatgt ttatctaccg ttctctgaaa gagccgtcgc
     2401 catggggtgg cggagtagtc gccaccaact tggggtcatg cctagggcaa cagctgctaa
     2461 cgctatggcg gccatggccg ccgcgaccga caagtcgaat cttggttcaa ttgccccgag
     2521 attggccatc agcgacggca gcgcaaattc cgtgggccga aagaatacag ccattaggac
     2581 ggccgtgatg gtgccggcga aagcgacggc caatcccaga ctgagaccta cgagagccac
     2641 ccgcgctgga ggcactccga gcgcaatcaa tatggcgtag tggcagcggc gatccgcgta
     2701 gacatcgtgc aagagattag ctgcgaatac acccgcgaac agcgcggtcg ctgtgccgat
     2761 aagcgtaaac aaactcagcg tcagcacaaa atcatgcacc ttgcggctga attggctgta
     2821 gcgttcggtg acgaaaaccc ccggttcaag tcggaaagct tcgcgtagac tgtcggcgag
     2881 ggagggcacg tcggtgggtg ttcgagcgac tacaagcgcg tccgtcaacg tcgtgcggcc
     2941 gaagattgcc tcacttgtac gcagcgtaac gaacacgccg ctcgatacaa agcttgaatt
     3001 ctgcgtgagc gcgggtgctt cgacagtacg aggcggtgca tcgcgcagct caagtttccc
     3061 gatgatgccg actaccgtca ggtccaggtc ttcgccgtcc gccttgcgga caggaaaagt
     3121 cttgccaaga tcaacaccaa gcgcgctggc agacacctca tccaggacgg caacaacgcc
     3181 gtcgtcactg cgtagaaagc gaccccgggt cagcgaaaaa ctactcacct gaccttccgc
     3241 gaccggatca attccgaaga tgtagacgtt caggaaacga tcgcccgaga ccacattgcg
     3301 gaacaatgat tggcgcggaa tgatcacgcc gtcaccggca gccgaactga gctgctcggg
     3361 ttcgatcagc ggttcataga ctggcagggt aaggggcggc aagttgccgc tgcgtggcgt
     3421 ctcgaacaga gcgcgattga aacgactccg tatctgcacg atttccggac gatcattccg
     3481 ctcagcctgc atcagagcgt attgggcgcc ctcgacgacc atcagcgagc ctgcgacgag
     3541 tgcgctggca gccgctagga caaggcctgc gaccaccgcc tcgaaaccgc gccggcctaa
     3601 cccgccgaat atgatcttgg tccaaatcat gcctgatcgc cgatcagctc ggctggagac
     3661 cgcgacagtg gccacagcag gaccgatagc gcggccaatc ctccaactac aaccgacaca
     3721 gtcgccgcca ggccaacgga tgcaagggac gagcccagcg aggatgtgac cgcaatcgac
     3781 gcgatcaccg cgaatgcaat tccgccgcca agcagcaagc agagttggac accgtagtag
     3841 cccacaattt gacccggcga gaagccgata gcctgcagca ctccgaactc gcgccaccgt
     3901 gtcacgacgg tctgtacctg caggccggcg ctccccaccg cgacaagggc aatcaggaga
     3961 gcgatgaaga acgatccgcc cgccccaacc ttgctctgtt cctgaaatgc agcctggaag
     4021 ttgttgcgct ccggcaacct gaggcaaaac gatctcggcg ttgatccctc gcagtcttct
     4081 gtcagctgtc gctctttcaa gagtgatttc acagccgtc
//
LOCUS       DATLJT010000856         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1433990,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000856 DATLJT010000000
VERSION     DATLJT010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433990"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VM910_19635"
     CDS             complement(<1..464)
                     /locus_tag="VM910_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI64774.1"
                     /translation="MGLRDSAIVAYAETKVMDKSDRDVWVLSGEILEELLDKTGVDKN
                     EIDGLVMAGLTGTGAGNPFWAQTTADVLGLEVGFCEQVHTGGCSAAGCVARAAAAIDY
                     GMCEVAFLLFADTHVLEDHTDHSHSYRREWTDPYGLMGPPGAFGLLSRRYEA"
     gene            complement(506..1291)
                     /locus_tag="VM910_19640"
     CDS             complement(506..1291)
                     /locus_tag="VM910_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVI64775.1"
                     /translation="MSEEQTVTYELEGETALVGLNRPDKRNCFNPTVMRQLREAIDRA
                     GEEAKCGIIFGHGDNFCAGLDLRWAAESWKNGRSERLPFPFNRNTFFEAMARGNVPFI
                     AALHGATLGGGLETAAAAHIRVADETTFFALPEGTRGIFIGGGGSVRVARLLGFARMQ
                     DMMLTGRVLKPDEAERYGVVQYVVSKGQSMIKAKELAAKICKNAPLSNFAITNSLPRL
                     QDMSYDDGLFFERMVAEYTRSPESIERLHQFLNKTAPRVSMPD"
     gene            complement(1356..2450)
                     /locus_tag="VM910_19645"
     CDS             complement(1356..2450)
                     /locus_tag="VM910_19645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI64776.1"
                     /translation="MELHLTSEQTLLRDSAAKFIAAAGPKVARGFRGQDPGFAPARLR
                     QAGELGWLGILVPSSMNGLGLGLTELALVLEQAGRGLVCEPIGLATISAAALGLGRTP
                     HPMLEPVMTGAAFVAPALEESAYGDDPLAPRTQVAGKNGTLRLTGQKMFVCADGADGF
                     LVSASGRDGPALCYVARNAPGCTLSTSQTAEGRKLATLSLADVPGDLIAPHSSSPNAV
                     DVLYNLALVALSSELLGAMERAQEITLDYLRIRKQFGKLIGSFQALQHQAVNIYIRIE
                     AARSLVYQVAADNDAYRIDPAMAAAVKAKVSEEAMVVTKACIQLHGAIGFTDEHDIGL
                     YLKRAMLLSSLFGNAAAQRRRYAATAGLTA"
     gene            complement(2470..2580)
                     /locus_tag="VM910_19650"
     CDS             complement(2470..2580)
                     /locus_tag="VM910_19650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI64777.1"
                     /translation="MDVAAAFLQSRRATIYGGSSEIQRNVLARRVLNLPS"
BASE COUNT          453 a          864 c          809 g          474 t
ORIGIN      
        1 tgcgcctcgt agcggcggct gagcaggccg aaggcgccgg gcggccccat caggccatag
       61 gggtcggtcc attcgcggcg gtaggagtgg ctgtggtcgg tatggtcctc cagcacgtgg
      121 gtgtcggcga acagcaagaa cgcgacctca cacatgccat aatcgatcgc cgccgccgcc
      181 cgggcgacgc agcccgccgc cgagcagccg ccggtgtgca cttgctcgca gaaaccaacc
      241 tcgagcccca gcacgtcggc cgtggtctgc gcccagaacg gattgccggc gccggtgccg
      301 gtcaggcccg ccatcaccaa gccgtcgatc tcgtttttgt cgacgccggt cttgtcgagc
      361 agctcttcga gaatctcgcc cgacagcacc cacacgtcgc gatcgctctt gtccatgacc
      421 ttggtctcgg cgtaagccac aatcgcgctg tcacgcaaac ccattgcccg ctctccctga
      481 aaatttttgg cccattgcgc gaatgtcagt ccggcatgct gacccgcggc gcggttttgt
      541 tgaggaactg atgcagccgc tcgatggatt cagggctgcg ggtgtattcc gccaccatgc
      601 gctcgaagaa cagcccatcg tcgtaggaca tgtcctggag gcggggcagg ctgttggtga
      661 tggcgaagtt cgacagcggc gcgttcttac agattttcgc ggcaagctcc ttcgccttga
      721 tcatcgactg ccctttcgac acgacatact ggacgacgcc gtagcgctcg gcttcgtcgg
      781 gcttgagcac gcggccggtc agcatcatgt cctgcatgcg ggcaaagccg aggagccgcg
      841 ccacgcgaac cgagccgccg ccgccgatga agatgccgcg cgttccctcg ggcagcgcga
      901 agaacgtggt ctcatccgcg acgcggatgt gcgcagccgc ggccgtctcg agtccgccgc
      961 ctagcgtcgc accgtgcagc gccgcgatga agggaacgtt gccgcgcgcc atcgcttcga
     1021 aaaaggtgtt gcgattgaac ggaaacggta gtcgctccga gcgtccgttc ttccaacttt
     1081 ccgcggccca gcgcaggtcg agcccggcgc agaaattgtc gccatggccg aagatgatgc
     1141 cgcatttcgc ttcctcgccg gcgcgatcga tcgcttcgcg caactgccgc atcacagtcg
     1201 gattgaagca gttgcgcttg tccggacgat tgaggccgac gagagcggtc tcgccctcca
     1261 gctcgtaggt gaccgtttgt tcttcgctca tgaactgctc ctggtttcaa cgggcaaatc
     1321 tagtccccgc cgggcgatca agtttctctg gaacgctatg ccgtcagacc ggcggtcgcg
     1381 gcatagcggc gtcgttgcgc cgccgcgttc ccgaacagcg acgacagcag catcgcccgc
     1441 ttgagataga ggccgatgtc gtgctcgtcg gtgaatccga tcgcgccgtg aagttggatg
     1501 caagccttgg tgacgaccat tgcttcttcc gacaccttgg ctttgaccgc ggcggccatc
     1561 gcaggatcga tgcggtaggc gtcgttgtcg gccgcgacct gatacaccag cgaccgcgcc
     1621 gcctcgatcc gaatatagat gttgaccgcc tgatgctgca gcgcctggaa gctgccgatc
     1681 agctttccga actgcttgcg gatgcgcagg tagtcaagcg tgatctcttg cgccctttcc
     1741 atcgcgccaa gcaattccga ggacagcgcc acgagcgcga ggttgtaaag cacatccacc
     1801 gcattgggcg atgatgaatg cggcgcgatg agatcgcctg ggacatcggc gaggctgagc
     1861 gtcgcaagct tccggccctc cgccgtctgc gacgtcgata gcgtgcagcc aggcgcattg
     1921 cgcgccacat agcaaagcgc gggtccatcg cgcccgctgg cgctgacgag aaagccgtcg
     1981 gcgccgtcgg cgcagacgaa catcttctgt ccggtcaaac ggagagttcc gttctttccc
     2041 gccacttgag tgcgtggcgc aagtgggtcg tcgccgtacg cgctctcttc gagcgccgga
     2101 gcgacgaatg ccgcgcctgt catcactggt tccagcatcg gatgcggcgt gcgccccagc
     2161 cctagcgcag ccgccgagat cgtcgcaagt ccgattggct cgcagacgag cccgcgcccg
     2221 gcctgttcaa gcaccagcgc gagctcggtg aggcccagac cgaggccatt cattgaactc
     2281 ggaacgagga tgccgagcca tccgagttcc cccgcctgcc gcaaccgtgc cggcgcaaag
     2341 cccggatcct gcccgcgaaa tccgcgcgcc accttcggcc ccgccgcggc aataaatttg
     2401 gcggcgctat cgcgcaacag cgtctgctcc gatgtcagat gcagctccat gacccgatcg
     2461 tgccgtccgt caactcggaa ggttcaacac gcggcgggcg aggacgttgc gctggatttc
     2521 cgatgagccg ccgtagattg tcgcgcggcg cgactgaagg aacgctgctg ccacatccac
     2581 ctcgccgaaa ttgctagcga
//
LOCUS       DATLJT010000857         8645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3731805,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000857 DATLJT010000000
VERSION     DATLJT010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731805"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VM910_19655"
     CDS             complement(<1..183)
                     /locus_tag="VM910_19655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HVI64778.1"
                     /translation="MSRCAFLAAALIVLLPAGPCFAVDAKQKMATCQFGADQQRLQGE
                     ARDAFLKKCMEDRDDPR"
     gene            844..2184
                     /gene="clcA"
                     /locus_tag="VM910_19660"
     CDS             844..2184
                     /gene="clcA"
                     /locus_tag="VM910_19660"
                     /inference="COORDINATES: protein motif:HMM:NF003640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HVI64779.1"
                     /translation="MDREPEVADESAQSRARLVALALLALLVGAGSGLIGAAFRLSLE
                     HADRLRDLLIVWAHGQRLVGFLLVTVGCAVAVAAAAWLVRRFSPHASGSGIPHVEAVL
                     QAALPPAPFSLMPVKFFAGVIAIGSGLALGREGPSVQMGAGVGYLIGRICRCNWPDCR
                     VLLAAGAGAGLATAFNAPIAGSIFVLEELVRRFEHRIAIAALGASATAIAVARLLLGD
                     APDFHVQALALPGAETAPLYFLLGAVAGLLAVLYNRTLLATIALADELTAWPVELRAG
                     LIGAAVGMLAWFAPEWVGDGNAITQRTLAGGETPALLPAIFVLRLGLGAVSYAAGTPG
                     GLFAPMLVLGAQLGLFFGLLSRLAIPDLTIQPEALAVVGMAAFFTGVVRAPLTGIVLV
                     TEMTASVTMLLPMLAACFVAMLVPTLLRDPPIYDSLRELTLAAAGKQSRGASVS"
     gene            complement(2426..4240)
                     /locus_tag="VM910_19665"
     CDS             complement(2426..4240)
                     /locus_tag="VM910_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVI64780.1"
                     /translation="MLSTQSAGMLMDVPSREFVFAGSFAELKAKGRLVLHGDHRPILV
                     IYDRGRVFALDNRCPHMGFPLERGSVEDGILTCHWHHARFDLESGCTFDLWADDVPKF
                     PVELRDGEVWVKTTLGHADPAVHWRQRLADGLAHDLGLVIAKAVHGQLAADVPPADIV
                     RQVALFGAQNRDGWGVGLTILAAFANLLPRLPEDETYLALFHGARRVAADCDGQAPRR
                     ERAPLGSHPDAGTLKRWLQRWTTVRHREGAERTVLTAIEGGLSPAVLADALLAAGTER
                     AYADTGHSVDFINKGFECLDLIGWEHAAAVLPTVVGQMVEARGAEESTAWRQPDDLVA
                     LCDNAESELPNLFRAGHRMHGWSDHLALTGELLGDDPVKIIDALKGAIRAGASPSDLS
                     RSLAYGAALRVARFGNANEHSDWETAHHVFTYSNAVDQMLKRIGSANVDADPTLVRGV
                     LHGAMALYLTRYLNVPPARIPGDAGEHLDDLPADIRKIRAALLDAFDRQRQVDLAARL
                     VARHLKLGHSPQALITTLAGGVLREDAGFHAYQMLEASVRQFTAWPNTDAGRHILIAV
                     ARYLAAHSPTERAALQTADIARRLMRGGELHQEEGASS"
     gene            4395..5378
                     /locus_tag="VM910_19670"
     CDS             4395..5378
                     /locus_tag="VM910_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64781.1"
                     /translation="MKLPRRKLLRLAAGAATLPSLSFVASAEDYPTRPIRIIAGFAAG
                     GAVDIVARLIAQWLSERIGQQCVVENRPGAGNNIATEYVLKSDPDGYTLLLANPTNAI
                     NATFYENLPYDFIRDSAPVAGIMRVPNVMEVSPLLSAKSVPEFITLAKANPGKLVYAT
                     GGNGTSVHMSAELFKLMTKTDILNVTYRGLALAYTDLMMNRVQVTFDNLPGSIGFIRQ
                     GKLRALAVTTTTRSPALPDIPTLAEFIPGYEASAWYGVSAPRNTPKAVIDKLNKEINA
                     GLADPALQARLVDLGGMIVPGSPADFGKLVSDETEKWAKVIKFAGIKPSQR"
     gene            5669..6706
                     /locus_tag="VM910_19675"
     CDS             5669..6706
                     /locus_tag="VM910_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HVI64782.1"
                     /translation="MKDVLLPTSLVGSYAQPDWLIDRQKLAGRFPPRVRARELWRIAP
                     EFLKEAHDDATLLAIRDQERAGLDIITDGEQRRESYSNRFATALEGVDIDNPGTALDR
                     SGHPNPVPRIIGKIRRRQPVEVRDVEFLRANTDRAIKITVPGPFTMSQQAQNDFYKDE
                     QEMVLDYATAVNAEIKDLFTAGADIVQIDEPYMQARPEKARQYGLRGLNAALEGVIGT
                     TAIHICFGYAAIIHVRPSGYSFLPELAASPVKQISIETAQSKLDCSVLQKLPGKTIIL
                     GTLDLSDVSIESPETVAARIRRALPHVPADRIVIAPDCGLKYLPREVAYGKMRAMVEG
                     AKIVRQELSAK"
     gene            complement(7049..7972)
                     /locus_tag="VM910_19680"
     CDS             complement(7049..7972)
                     /locus_tag="VM910_19680"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HVI64783.1"
                     /translation="MRIAVIGAGAVGGSFGAALAKAGNEVTFVARGAHLAAMRARGLS
                     VLGPRGDIHLNPVQATDDPASIGPVDYVMFCVKLWDVESAGAAVRPLVGPITAVIPLQ
                     NGIDASERLIPILGKDAVMGGVAQISATVAEPGVIRQTGTFMRLVFGELDGRPSQRGA
                     AFHALCRGAGFDSTNTSEIIVALWEKFVLLATNSSVSTLTRLPFGKLRDDPDVFALFE
                     KTTAEVAAVGRARGIRLPADIEARTLQTIRNFPPEMMPSMAVDLLRGNRLELPWLAGK
                     VVALGREVGVPTPTFDVMYAALKPYANGAPA"
     gene            complement(8040..>8645)
                     /locus_tag="VM910_19685"
     CDS             complement(8040..>8645)
                     /locus_tag="VM910_19685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64784.1"
                     /translation="DDIQRMLDLAYQRWGRLDVLFNNAGIAEVRQLLELTEDEWDRTL
                     AVNLRAVFFVLQGAAKRMRHQSPIAASELRGKLIQTASIAAYRGGQAYMVHYAASKAG
                     VVSITRTAAQVLATDRITSNCVCPGAVDTPMWSKIDAEWTAIEGWEIREAWKRRTSLI
                     PLGRPETPEDVAGVVSFLASRDSDYMTGQAVNIDGGLVMGN"
BASE COUNT         1659 a         2716 c         2599 g         1671 t
ORIGIN      
        1 gcgcggatcg tcccgatcct ccatgcattt cttgaggaaa gcatcacgcg cctcgccttg
       61 cagtcgttgc tggtccgcac cgaactggca ggtcgccatt ttctgcttgg catcgactgc
      121 aaaacacggt cccgccggca atagcactat cagagcggca gccagaaacg cgcaacgaga
      181 catgctgaac cccccgaatc ccttaaaact acagatccac atcaaagcaa agaattggcg
      241 ccaaaaattg gcaccgaacc ctataaaatc tccgaggacg cttaggcacg accattttcg
      301 cgttaacgct taaggcttcc gcaacagctc cggcacttcg ccccacccga tgttcgcttt
      361 gggtcaaaag cagacaacgg gttgccgcct attgatgtcc gctttacccc caaaagcgga
      421 catggttcag cccgatcgcg atgtccgctt tgtgccaaaa tcagactcat acactgcagc
      481 aatcaatgcc cggcttatgc cggattttcg ccaatagttg acgtgggcag gtattctctc
      541 aggcaatcgt tacgctgagc atttgtgcag atgtctgcaa acaggcagtg aggatcggaa
      601 cgatctaccc tcatctttgt gcagacgtct tcacagtgag gcagccggcg acggaaagtt
      661 gatgaaacca gtttgtgcag ttggctgcag aacgctatgc acatgcccga acatgtagct
      721 gcgggctgaa aagaggtagt aagtgcgctt tcattgggag tacgtaaccc gccttgacgg
      781 gaggattccg attaccgtta cttgggaccg cggtttcaac tgccttccag atcgtgcggg
      841 tcgatggatc gcgaaccgga ggtcgccgac gagagcgccc agagccgcgc ccgcctagtg
      901 gcactcgcac ttttggcact gcttgtcggc gcgggatcgg ggctcatcgg cgcggccttt
      961 cgcctttcgc tcgagcacgc cgaccgcttg cgcgacctgt tgattgtctg ggcgcacggc
     1021 caacggctcg tcggcttcct gttggtgacc gtcggatgcg ccgtcgccgt cgccgctgcg
     1081 gcctggttgg ttcgccgctt ttcgccgcac gcctcaggga gcgggattcc ccatgtggag
     1141 gcggtcctgc aggccgcgct gccgccggct cccttcagcc tgatgccggt gaagttcttc
     1201 gccggcgtca tcgccatcgg ctccggactt gcgctcggcc gcgaaggtcc gagcgtgcag
     1261 atgggcgctg gcgtcggcta tctgatcgga cggatatgcc ggtgcaactg gccggactgt
     1321 cgagtcctgt tggcggccgg tgccggcgcc ggtctcgcga ccgccttcaa tgcgccgatc
     1381 gccggctcga tcttcgtgct ggaggagtta gtgcggcgat tcgagcaccg gatcgccatc
     1441 gccgctctcg gggcgtcggc aaccgccatc gcggtggcgc ggctgctgct cggcgacgcc
     1501 ccggactttc acgtgcaggc gctggctctt cctggcgccg aaacggcgcc actctacttc
     1561 ttgctgggtg cggtcgccgg gctattggcc gtcctctaca accgcacgct gctggcaacc
     1621 attgcgttgg ccgacgagct caccgcctgg ccggtggagt tgcgtgccgg cttgatcggc
     1681 gcagcggtcg gcatgttggc gtggttcgcg cccgagtggg tcggcgatgg caacgcgatc
     1741 acccagcgca cgctggccgg cggcgagaca cccgcgctcc tgccggcgat cttcgtgctc
     1801 cggctcgggc tcggcgcggt gtcgtacgcg gccgggacgc caggcgggct gtttgctccc
     1861 atgctcgtgc tcggcgcgca actcggtctt ttcttcgggc tcctctcgcg gcttgcgatc
     1921 cccgacctca ccattcaacc cgaagcactt gccgtcgtcg gcatggccgc cttcttcaca
     1981 ggtgtggtgc gggccccgct caccgggatc gttctggtca ccgagatgac ggcaagcgtc
     2041 acgatgctgc ttcccatgct ggccgcgtgc ttcgtcgcga tgctggtgcc gaccctgctg
     2101 cgcgatccgc cgatctatga ttccttgcgc gaactgacgc tcgctgcggc gggcaaacag
     2161 tctcgcggcg catcagtcag ctgatttact cgtcacgacg agcaaagccg ttcccgaaaa
     2221 tggcaaccta ctagggacac gcgccgatga actctgctga tgaaaaggct gccccgagcg
     2281 gaccggcgaa ggtacgttct gacgacccga aacacgcatt gggcctggcc caatacctgc
     2341 gaactatcgg ttacgacgcc tggattgagg acacaaacgg caatgcgatc gacgaaaagg
     2401 ccctacagat ggcgattagg tctcgtcacg aggatgcacc ctcctcctga tgcagctcgc
     2461 cgccccgcat tagccggcgg gcgatgtcgg cagtttgtaa ggctgcgcgt tcggtcggtg
     2521 aatgggccgc aagatagcgg gcgaccgcga ttaggatatg tcggccagcg tcagtattgg
     2581 gccaggcagt gaactgccga acgctcgcct ccagcatctg ataggcgtgg aagcctgcat
     2641 cttcgcgcaa aacaccgccg gcgagcgtgg tgatcagcgc ctgcggcgaa tggccgagtt
     2701 tgaggtgtcg tgcaactagt cgagctgcga gatcaacctg tcgctgccgg tcgaaagcat
     2761 cgagtaaggc ggcgcgtatt ttccggatgt ccgcgggcag gtcatcgagg tgctcgccgg
     2821 cgtctcccgg gatgcgagct gggggcacat tgagatagcg cgtaagatag agcgccatcg
     2881 caccgtgcag aacgccacgc accagcgtcg ggtcagcgtc aacattggcg ctccctatgc
     2941 gcttgagcat ttggtcgacc gcattggaat aggtgaagac gtggtgcgcc gtctcccagt
     3001 cggagtgctc gttggcattg ccgaagcggg cgacccggag cgctgccccg taagcgagtg
     3061 atcgactaag atcagaaggg gaggcgcctg cgcggatagc ccccttgagt gcgtcgatga
     3121 tcttgaccgg atcgtcgccg agcagctcgc cggtgagcgc taaatgatcg gaccagccgt
     3181 gcatacggtg accagcacga aataagttcg gcaattcgct ttctgcgttg tcgcacagcg
     3241 cgacaagatc atcgggttgg cgccaggcag tcgattcctc ggcgccacgg gcctccacca
     3301 tttgaccgac aacggtgggc agcacagccg ccgcatgctc ccagccaatc aggtcgaggc
     3361 actcgaaccc cttgttgatg aagtcgaccg agtgtccggt gtcggcgtat gcccgttcgg
     3421 tgccggccgc cagcagagcg tcggcgagca ccgctggaga tagaccgccc tcaatcgcgg
     3481 tgagtacggt gcgctcagcc ccttcgcgat ggcgcacagt agtccaacgc tgcagccatc
     3541 gcttgagcgt gcccgcatca gggtgacttc caaggggcgc acgttctcgt cttggtgcct
     3601 gcccgtcgca gtccgccgcc acgcgtcgcg ccccatgaaa tagggcgaga taggtttcgt
     3661 cttccggcag cctaggcagc agattggcga acgccgctaa gatggtgaga cccacgcccc
     3721 aaccatcgcg gttctgcgct ccgaacagcg ccacctgccg aacaatatcg gcaggcggta
     3781 catcagccgc gagctgacca tgcaccgcct tggcgatgac gagccccagg tcgtgggcga
     3841 ggccgtctgc gagccgctga cgccagtgca ctgcgggatc ggcatgaccg agcgtggtct
     3901 tcacccagac ttcgccatca cgcaattcga ccgggaactt cggtacatcg tctgcccaca
     3961 gatcaaaggt gcagccgctt tcgagatcga agcgggcgtg gtgccaatgg caagtcaaga
     4021 ttccgtcctc gacgctgccg cgctccagcg ggaagcccat gtgcgggcac cgattgtcga
     4081 gggcgaagac gcgaccgcgg tcgtagatga caaggatcgg tcgatgatcg ccgtgcagaa
     4141 caaggcgtcc tttcgccttt agctcggcaa agctgccggc gaacacgaat tccctactcg
     4201 gcacgtccat aagcattccc gcgctttggg tactcaacat aggtattctc tagcgcaagc
     4261 gtcatctggt ggtcttgtgc atcacaacag aaacgcgcgc ccgatgtccg ctttgggtca
     4321 aaagcagaca tttacgccgc tcttggatat cattgatcgt gagtggggtg tgctcaagtt
     4381 cgtggagcat caccgtgaaa cttccccgcc gaaaattgct gcgtctggca gccggtgctg
     4441 cgacgctccc aagcctatct ttcgtcgctt cggctgaaga ctacccgacg cgcccaatca
     4501 ggatcatcgc cggcttcgcc gcgggcggcg ctgtcgatat tgtcgcgcga ctcatcgcgc
     4561 aatggctgtc ggaacgcatt ggccagcaat gtgttgtcga gaatcgtccc ggcgccggca
     4621 acaacatcgc cacggaatat gtgctgaaat cggatcccga cggttacacg ctactccttg
     4681 ccaatccgac caacgcgatc aacgcgacct tttacgaaaa tctgccctac gatttcatcc
     4741 gcgacagtgc gccggttgcc ggcatcatgc gcgtgcccaa cgtcatggaa gtcagccccc
     4801 tgctgtccgc caagagcgta cctgagttca taacgctggc gaaagcaaat ccaggcaagc
     4861 tggtctatgc cacgggggga aacggcacgt cggtgcacat gtccgccgaa ttgttcaagt
     4921 tgatgaccaa aactgacatt ctcaacgtga cctaccgagg gcttgcgctt gcatacacag
     4981 atctgatgat gaatcgggtg caggtgacct ttgataacct gccaggctcg attggattta
     5041 tacgccaagg caagctccgt gctttggccg ttaccacaac gacacgttcg ccggcgctcc
     5101 ctgatattcc gacgcttgcc gaattcattc ctggctatga ggcgagcgcc tggtacggcg
     5161 tcagtgcgcc gcgaaacacc ccgaaagcgg tgatcgacaa gctcaacaaa gagatcaatg
     5221 ccggcctggc cgatccggcg ctccaagcgc gacttgtaga tctaggcggt atgatagtcc
     5281 ccggctcacc tgctgatttc ggcaaactcg tcagcgatga gacggagaaa tgggcgaagg
     5341 tgattaagtt cgcgggcatt aagccgagcc agcgatgacg tgctgattga gaggcggacg
     5401 cggttacaaa tcaggtcaat ccatgaccat cgatttgacc agagatgaga tttggctcct
     5461 gaaggccttg cgagctgcag ggacgcgcgg tcgcatcgtt agcgcgcaag cctctcgtgc
     5521 cgctctcact cgcctagtgg aagtgcaatt tgtaacgaca catccgcaga gtgaacgaaa
     5581 gacgttgtac gttatcactg atcgcggccg acacgctctt caattaatcc atagggtcat
     5641 atcgagggct taacgggcac aagatgccat gaaagacgtg ctgttaccga cctcacttgt
     5701 tggctcctat gcgcagccag attggctgat cgaccgccag aagcttgccg ggcgctttcc
     5761 accgcgcgtg cgagcgcggg aactctggcg catagctccg gaatttctca aagaggcaca
     5821 tgacgacgca acgcttcttg ctatccgcga ccaggagcgc gccggcctcg atatcatcac
     5881 cgatggcgag cagcgccgcg aaagctactc caaccgcttc gctaccgcgc tcgagggtgt
     5941 cgatatcgac aatccaggta ccgccctcga ccggtccggc catccaaatc cagtgccgcg
     6001 catcatcggc aagatccgcc gcaggcagcc cgtcgaggtg cgcgacgtcg agttcctgcg
     6061 tgccaacacc gaccgggcca tcaagatcac tgtgcctggc ccattcacca tgtcgcagca
     6121 ggcgcagaac gatttctaca aagacgagca agagatggtg cttgactatg cgacggcagt
     6181 gaacgccgag atcaaggacc tgttcacggc gggcgccgat atcgtgcaga tcgacgaacc
     6241 ctatatgcag gcgcgacctg aaaaggcgcg gcaatacggg ttgcgtggat tgaatgcggc
     6301 tctcgaaggc gtcataggaa cgaccgcgat ccacatctgc ttcggctatg ccgctatcat
     6361 ccacgtgcgg ccgtcgggtt attcattcct gccggagttg gctgcgtcgc ccgtaaagca
     6421 gatttcgatc gagaccgcgc aatctaagct cgactgctcg gtacttcaaa agctgccggg
     6481 taagacgatc atccttggca cactcgacct gtcggacgtg agcatcgaga gcccggaaac
     6541 ggtcgcggcg cgtatccgtc gtgccctgcc tcatgtgccg gccgaccgca ttgttatagc
     6601 gcccgactgc ggcctaaaat acctgccccg cgaggtggcc tacggcaaga tgcgagcaat
     6661 ggttgagggt gccaagatcg tgcggcaaga attgagcgct aaatagagat ctagcgtccc
     6721 gaaagagttt ctgcaccgct tgcctcggct cacccgttct ctggttcaat cgatgtccgt
     6781 tttacccccg aaagcggaca ttcctcagcg ctgtctcgat gtccgctttg tgccaaaagc
     6841 ggacatcgct gcgacgccac ttcgctcggt gaacaaggca aagccagcga ccacaaagtg
     6901 gctggcttgc tatgcgtcaa atcggcagca tcttgccaac attgtgaaga cgctctcagc
     6961 cttacccttg ctcagtaggc caacgaaagc gccaccgaac atcaatggca acggaccgac
     7021 tttgctaggc tgtgtacttg cgtcgatttc acgccggcgc gccgttggcg tagggcttga
     7081 gcgccgcgta catcacgtcg aacgtcggcg tcggcacgcc gacctcgcgt cccagggcca
     7141 ccactttgcc ggcaagccac ggcagctcca ggcggttgcc gcgcaacaga tcgaccgcca
     7201 tcgacggcat catttccggc ggaaaattgc gaatggtttg cagcgtccgg gcctcgatat
     7261 ccgctggcaa gcggatgccg cgggcgcgac ccaccgccgc cacctcggcg gtggtctttt
     7321 cgaacaaggc aaacacgtcg ggatcgtccc gcaacttgcc gaacggcaac cgcgtcagcg
     7381 tcgacacgga gctgttggtg gcgagcagga cgaatttttc ccacagcgcg acgatgattt
     7441 cgctggtgtt cgtcgaatcg aatccggcgc cgcggcatag cgcatggaat gccgcgccgc
     7501 gctgactggg gcggccgtcg agttcgccga acacgaggcg cataaacgtc ccggtctggc
     7561 ggatcacgcc cggctcggcg acggtggcgc tgatctgtgc aacgccgccc atcaccgcgt
     7621 ccttgccgag gatggggatc agccgctcgc tggcgtcgat tccgttctgc agcgggataa
     7681 ccgcggtgat cggtcccacc agcggccgca cggcggcgcc ggcactttcc acgtcccaca
     7741 gcttcacgca gaacatgacg taatcgaccg ggccgatgct ggccggatcg tcggtagcct
     7801 gaaccgggtt gagatggatg tcgccgcggg gcccgagcac ggagagcccg cgcgcgcgca
     7861 tcgccgccag atgggctccg cgcgcgacga acgtcacttc gttgccggcc ttcgccaggg
     7921 ccgctccgaa cgatccgccg accgcgccgg cgccgatcac cgcgatccgc accgtgtcag
     7981 tccctgttgt catgttgctt tgcgctctct agacttgtca gcctcgcctc catctggcct
     8041 cagttgccca taacaagacc gccgtcgatg ttgacggcct ggcccgtcat gtaatcggag
     8101 tcacggctgg caagaaagct cacgacgcca gccacgtctt caggagtttc gggtcgtccg
     8161 agtgggatca gcgaggttcg ccgcttccag gcttcacgaa tttcccagcc ctctattgcg
     8221 gtccactcgg cgtcgatctt gctccacatc ggtgtgtcga ccgcaccagg acagacgcaa
     8281 ttcgaagtga tcctgtcggt cgccagcacc tgcgccgcag tgcgcgtgat gctgacaacg
     8341 ccggctttgc tagcggcgta atggaccatg tacgcctgac caccccggta ggcggcgatc
     8401 gacgcggtct ggatcaactt tccgcgcagc tcagaagccg caatcgggct ctgatggcgc
     8461 atgcgtttgg cggcgccttg caacacgaaa aaaacagcac gcaggtttac cgccagggtg
     8521 cggtcccact cgtcctcagt gagctcgagc agttgccgga cttcggcgat ccccgcgttg
     8581 ttgaacagca catccaggcg accccagcgc tggtaggcca gatcgagcat acgctgaatg
     8641 tcgtc
//
LOCUS       DATLJT010000858         3702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3502308,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000858 DATLJT010000000
VERSION     DATLJT010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502308"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1289
                     /locus_tag="VM910_19690"
     CDS             222..1289
                     /locus_tag="VM910_19690"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI64785.1"
                     /translation="MDFGIQLATSSHSWKVVKRAEELGFTHAWFYDTQLLNADVFVAM
                     AAAAMQTSRIRLCTGVLIPSNRIAPVAASALASLNALAPGRIHFGVGTGFTARRTMGL
                     RAITLARLGEYVRVVQGLLKGETVEWSAEGRSHKIRFLNPELELINLHDPIPVHISAF
                     GPRGRSLTAKLGAHWITAMRGAQPAIAALAEMQKAWQEAGRDLKALYSTAFGGGCVLA
                     EGESADSARAKALAGPAAAIVFHNKAEEQELGSIGFPTPPQFKTKFDAYGEIYRKYQP
                     PDARYLSNHRGHLMFLRPEEHDIISADVIRAFTFTGTRAELADGLRGMKAAGYSQFGM
                     HIRTGHEIAMLEDWAEVIASV"
     gene            complement(1534..3333)
                     /locus_tag="VM910_19695"
     CDS             complement(1534..3333)
                     /locus_tag="VM910_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HVI64786.1"
                     /translation="MPEPSSEATSGEFNPEQKRYLEGFVSGLQIARAARAASGNGSDG
                     AAAAEPVGPDAAHLRAQDRFVKEGKKLSDPEKFKRELHPFDGYERLKVQAANNEPPKA
                     DDNFRWRFYGLFYCAPNQAAYMCRLRIPNGILKHWQFAGVGDLAERYAGGYSHVTTRA
                     NLQMREVEPKNAVAIVEAVQDLGLCSRGSGADNIRNVTGTPTAGIDPQELIDTRPCAR
                     EWHFHILNDRSLYGIPRKFNVGFDGAGVIPVLEDTNDIGFQVVEVKDGFGVEPGIWFR
                     LLLGGITGHQTFAHGTGVVVKPSEATKVADAVVRVFIDHGDRTNRAKARLKYVIDAWG
                     IDKFLSATEDKLGCKLVRAPAEAIAPRPAFDRTAHVGVHPQKQPDLNWVGVALPVGRL
                     TGEQMRGLAAIARDYGDGDIRLTVWQNLLISGIPTQRIESVEAAIAALGLSTKATAIR
                     AGLVACTGNAGCRLALSNTKRHAEDIAQWCETRLQLDTPVNIHLTGCPNSCAQHYVGD
                     IGLVGARVPISEDGDTVDGYHIHVGGGFGPDQSLGREIYRDVKAEDAPTTVERMLKAY
                     LAHRASERETFAAFTRRHEVGALKAMFEREAVE"
     gene            complement(3370..>3702)
                     /locus_tag="VM910_19700"
     CDS             complement(3370..>3702)
                     /locus_tag="VM910_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HVI64787.1"
                     /translation="CNWMVCLAVVLAMGTNSTIGKIATAWMPIFIFFAQGFEHAVVNM
                     FIIPTGMLLGAKVTWSDWWLWNQIPVTLGNIVGGFVFTGLAIYLTYKPRPTQEGSPLP
                     VPTRVPAE"
BASE COUNT          667 a         1140 c         1191 g          704 t
ORIGIN      
        1 gtccgcccga attgtttgcc ggcacgaaca cacttcacac cggcgggcag caccaatcct
       61 atcttttgct gccggtgttg cccatgtgat agatcggcac gatcggacgg aaagccacct
      121 ctgcttggaa ataagagcga gctatgagca cgaccaagat gagctcggcg cgagcgaggt
      181 gcttgtgtgt ctagtttcgg tatacaacaa gggaggggat tgtggatttc ggcattcagc
      241 ttgcgacctc atcacactcc tggaaggtcg tcaaacgtgc ggaggaattg ggcttcaccc
      301 acgcttggtt ctacgacacc caactcctga atgccgacgt tttcgtcgcc atggcggcgg
      361 cggccatgca aacatcgcgc attcggctat gcaccggggt attgattccg tccaatcgga
      421 ttgctccggt tgcggccagc gcgctggcat cgctgaatgc gctcgcccca ggccggattc
      481 atttcggcgt cgggaccgga tttaccgctc ggcgaacgat gggacttcgt gcgattacgc
      541 tcgcgcgact gggtgaatat gttcgcgtcg tccagggtct gctcaagggg gagacggtcg
      601 aatggtcagc ggaaggacga tcccacaaga tccgcttcct caaccctgaa cttgaactca
      661 tcaatctgca cgatccgatc cctgtgcaca tttccgcctt cggaccgcgt gggcgttcgc
      721 tgaccgcgaa gctcggtgct cactggatca ccgcgatgcg aggcgcgcag ccggcgatcg
      781 cagcactcgc cgaaatgcag aaggcctggc aagaggccgg ccgcgatttg aaggcgctct
      841 attcgacggc cttcggcggt ggttgcgtgt tggctgaggg cgagtcggcc gacagcgcgc
      901 gcgcgaaggc cctggccggt ccggcggcag cgatcgtgtt tcacaacaaa gccgaagaac
      961 aagaacttgg ctcgattggt ttcccgacgc ccccgcaatt caagaccaag ttcgacgcct
     1021 atggtgaaat ctatcgcaag tatcagccgc cggatgcacg ctacctctct aaccaccgcg
     1081 gccatttgat gtttctgcgt ccggaggagc atgacatcat cagcgccgac gtcattcgcg
     1141 ccttcacgtt caccggcacg cgcgcggaac ttgcggatgg attgcgaggg atgaaggcgg
     1201 cgggctacag ccagttcgga atgcatattc gcaccgggca tgaaatcgcg atgctggagg
     1261 attgggcgga ggtgatagcc agcgtatgaa ttggcggctc aatagacgtc ctgctgatag
     1321 cgaccctttt ttcgccgtca tcgagcggcc cgttcgcggc cggcgtcagg cccgacatca
     1381 gcacagcgtt cgcctccggc gaaagcgcgg tgatgcctgc gccgccaagc tcaaggaagc
     1441 cggcaaagaa gccgttgagc caagtccgct gctccggggt gaacggggcg ctgtcgggaa
     1501 ccaatttcgc aatcggcgga cgcgtcggtt gagtcattcg accgcctctc gctcgaacat
     1561 ggctttcaac gcgccgactt cgtggcggcg ggtaaacgcc gcgaatgtct cccgctccga
     1621 ggcgcgatgc gcgaggtagg ccttgagcat gcgctcgacg gtggtcggcg cgtcttcagc
     1681 cttgacgtcg cggtagatct ctcgccccag actctggtcg ggaccaaaac cgccgccaac
     1741 atggatgtga tagccgtcga cggtgtcgcc gtcttccgag atcggcactc tggcgccgac
     1801 gagtccgatg tcgccgacat agtgctgggc gcaggaattc gggcagcccg tgaggtggat
     1861 gttcaccggg gtgtcgagtt gcaagcgcgt ctcgcaccac tgcgcgatgt cctcggcgtg
     1921 gcgcttggtg ttggaaagcg cgaggcggca gccggcattg ccggtgcagg cgacgaggcc
     1981 ggcgcggatc gcggttgcct tggtcgaaag gccaagcgcc gcgatcgccg cttcgaccga
     2041 ttcgatgcgc tgcgtgggaa tgcccgagat cagcaggttc tgccagaccg tgaggcggat
     2101 atcgccgtcg ccataatcgc gcgcgatcgc tgccagcccg cgcatctgct cgccggtgag
     2161 ccggccgacc ggcagcgcca cgccgaccca gttcaggtcg ggctgcttct gcggatgcac
     2221 gcccacatgg gcggtgcggt cgaaggccgg ccgcggcgcg attgcttcgg cgggagcgcg
     2281 cacgagcttg cagccgagct tgtcctccgt cgcgctcagg aacttgtcga tgccccaggc
     2341 atcgatcaca tatttgaggc gcgccttggc gcgattggtg cggtcgccgt ggtcgatgaa
     2401 gacgcgcacg acggcgtcgg cgactttcgt cgcctcgctg ggcttcacca ccacgccggt
     2461 cccgtgcgca aaggtttggt gtccggtgat gccgccgagg agcagccgga accagatccc
     2521 cggctcgacg ccgaagccat ccttcacctc gacgacctgg aacccgatat cgttggtgtc
     2581 ttcgagcacc ggaatgacac cggcgccgtc gaagccgacg ttgaacttgc gcggaatgcc
     2641 gtagagcgag cgatcgttga ggatgtgaaa gtgccattcg cgcgcacagg gtcgcgtatc
     2701 gatcagctcc tgcgggtcga taccggcggt cggcgtcccg gtgacgttgc ggatgttgtc
     2761 ggcaccggac ccgcgcgaac acagccccaa atcctgaacc gcctcgacga tggcgaccgc
     2821 attcttgggc tcgacctcgc gcatctgcag attggcgcgc gtcgtcacat gggaatagcc
     2881 gccggcgtat cgctcggcga ggtcgccgac gcctgcgaac tgccagtgct tgaggatgcc
     2941 gttggggatg cgcaggcggc acatatatgc cgcctggttc ggtgcgcagt agaacaaacc
     3001 gtaaaatcgc cagcggaagt tgtcgtcagc tttcggcggc tcgttgtttg cggcctgcac
     3061 tttcaggcgc tcatagccgt cgaacggatg cagctcgcgc ttgaacttct ccggatcgga
     3121 aagcttcttg ccttctttga cgaagcgatc ctgcgcgcgc agatgagcgg catcgggtcc
     3181 cacgggttcg gccgcagccg ccccatcact gccgttacct gacgccgcgc gagccgcacg
     3241 cgcgatctgc aatcccgaca cgaagccttc cagatagcgc ttctgttcgg gattgaattc
     3301 gccggatgtc gcctctgagc ttggctcggg cacgagttta gtggctccct ttgcctctcg
     3361 ccgccctgcc tactcggcgg gaacgcgcgt gggcacgggc agcggcgagc cctcctgggt
     3421 tggccggggc ttgtaggtga gatagatggc aaggccggta aaaacgaagc cgccgacgat
     3481 gttgccgagc gtcaccggaa tctgattcca cagccaccag tcgctccatg tgaccttggc
     3541 gcccagcagc atgccggtcg gaataatgaa catgttgacg acggcgtgct cgaagccctg
     3601 cgcgaagaag atgaagatcg gcatccaggc cgtggcaatt ttgccgatcg tggagttcgt
     3661 gcccattgcc agcacgaccg cgagacaaac catccaattg ca
//
LOCUS       DATLJT010000859         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_55969,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000859 DATLJT010000000
VERSION     DATLJT010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55969"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VM910_19705"
     CDS             complement(<1..465)
                     /locus_tag="VM910_19705"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI64788.1"
                     /translation="MTVTIGRRELLVALGGTAAAWPIAARAQQPEQMRRVVVLMSTGE
                     DDPQDATRLAAFERGLQELGWVIGRNLNIDYRWAAGDPDSLRKYATELRVLAPDAIVA
                     SGTQAFTAAQQTTRTVPIVFVNIVDPVSSGFVSSLAQPGGNATGFLMFEYATS"
     gene            complement(810..1586)
                     /locus_tag="VM910_19710"
     CDS             complement(810..1586)
                     /locus_tag="VM910_19710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI64789.1"
                     /translation="MKLPRRTFLHPAAGAAALTLLLLEGLAADAAEVRVLSTNAITSV
                     TNELYAQFELVTGHKLTVRYEFGPILKREIEAGATFDVSILSLDVDDLIKQGKIAVGT
                     RAVLGRTGIGVGVRKGMPKPDITTIEAFKRALFNAKTIAYSRAGSSGLYFLGLLDRLG
                     IAEDLKAKLKPSTGNNTVEAVVTGEAELVVVGVALILMVPGAELVGWLPSELQSYIVF
                     TGGVSATAKEPEAAKALLDFLTTAETRAILEAKGLEPVTP"
     gene            complement(1630..1776)
                     /locus_tag="VM910_19715"
     CDS             complement(1630..1776)
                     /locus_tag="VM910_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64790.1"
                     /translation="MKRRAFITLLGGAVAWPLAARAQQTGQDAACRVHPGYPLPLICT
                     RDVA"
     gene            complement(1785..>2811)
                     /locus_tag="VM910_19720"
     CDS             complement(1785..>2811)
                     /locus_tag="VM910_19720"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64791.1"
                     /translation="CYPFCRKHGRYRAVKRRHFITLLGSAAAAWPLTARAQQGERVRR
                     VGVLMNIAADDADAPARVAALAQGLQELGWTIGRNLRIEYRWSAGEADRIRRYAAEIS
                     ALSPDVIIASGGAHAAAVQQAAPTVPVVFLSVNDPVAAGLVASLARPGGNATGFTGLE
                     YGTSAKWLELLKQIAPGVARVAVLRPANIGGVGQLGAIQAGAGSLGVELSPVGLRDVG
                     EIERSVAAFAREPNGGLIVVASALGLVHRALIVELAARHRLPAIYAFRVFVTAGGLIS
                     YGPDLADQYRRTASYVDRILKGEKPADLPVQAPTKYELVINLKTAKALGLEIPATVLA
                     RADEVIE"
BASE COUNT          554 a          892 c          843 g          522 t
ORIGIN      
        1 gctggttgcg tactcaaaca tgagaaaacc ggtggcgtta ccacccggct gcgccaagct
       61 tgagacaaag cctgagctga cgggatcgac aatgttgacg aagacgatcg gcacagtgcg
      121 ggttgtctgc tgtgcagcag taaacgcttg ggtgccactc gccacgatcg cgtcaggcgc
      181 aagtacgcgc aattctgttg cgtatttgcg taagctgtcg ggatcgccag cagcccagcg
      241 atagtcgata ttcaggttgc ggccgatcac ccaccccaat tcctgcaagc cccgttcgaa
      301 cgcagcgagg cgagttgcgt cttgcggatc atcttcgccc gtggacataa gcacaacgac
      361 gcgccgcatc tgctcgggct gctgtgcgcg cgccgcgatc ggccatgctg ccgccgtacc
      421 gccgagtgcg accagcaatt cccgccgtcc gatggtgact gtcatacgcc cctcccgtgc
      481 gaggtgcgta aaaggaagga taccacgcca cgagcgtgct gtcctaacag cgcggcaccc
      541 ggcgcgaacg gagcgtgttg atttcaagag atttattttc ggccgcgccg taggccgtgc
      601 actcataaac gcgggccgaa tgtgcgctct tggggcgaac cggacacgcc gggatggggg
      661 gaatgacctt gatgacccat ttgcgacatg ggaggtcgga atcgacgaac ggaatttaca
      721 aaatacctga cggggggtct gcggggttca gtcgggctta ccggcccgac acactttacc
      781 tgcgcttgaa gaaagtcatc cgacttaact catggcgtga caggctccaa acccttcgcc
      841 tcgagtatcg cacgggtctc tgccgtcgtc agaaaatcta acaatgcttt tgctgcttct
      901 ggttccttgg cagtggcgct cacgccacca gtaaagacga tgtaactctg caactcggat
      961 ggcagccacc cgacgagttc ggcgcctgga accatgagga ttagcgcaac tccgaccacg
     1021 acgagttctg cttcgccggt cacgacggct tctactgtat tgttacctgt tgagggcttg
     1081 agtttagctt tcaggtcctc ggcgatcccg aggcgatcaa gtagaccaag aaaataaagg
     1141 ccgctgctgc ctgccctcga ataagcaatt gttttcgcgt tgaacagtgc gcgcttgaat
     1201 gcttcgattg ttgtgatgtc tggcttcggc atgccctttc gcacgccgac acctattccc
     1261 gtgcggccga gcacggcacg cgtaccgaca gcaatcttgc cttgcttaat caagtcgtca
     1321 acatcgagcg acagaatgga cacgtcaaat gtcgcgccgg cttcgatctc gcgtttcaag
     1381 atggggccaa attcatacct gactgtgagc ttgtggcccg ttaccagctc gaactgagcg
     1441 tacagctcgt tcgtcactga ggttatggca ttggtgctca ggactctaac ttcagcggca
     1501 tcagccgcga gaccctctaa caaaagcaac gtcagcgcag cggcgcccgc tgctggatgc
     1561 agaaaagttc tcctgggaag tttcacggca gccccctccc acgaaagcag ccggacgatg
     1621 gaacgcgggc taggctacat cacgggtgca aatcagcgga agggggtagc ccggatgtac
     1681 ccgacacgcg gcgtcttgcc cggtctgctg cgcgcgcgcc gcgagcggcc aagcgaccgc
     1741 gccgccgagc agcgtgatga acgcgcgccg tttcatcgct gcaatcactc gatcacctcg
     1801 tcggcacggg cgagcaccgt cgccggaatt tcgaggccga gcgcctttgc ggtcttgagg
     1861 ttgatcacaa gttcgtactt ggtcggagcc tgcactggaa gatcggcggg cttctcgccc
     1921 ttgaggatgc ggtcgacgta ggacgcggtc cgccgatact ggtcggccaa gtcaggcccg
     1981 taggagatta ggccgccggc cgtaacgaag acccggaaag catatatcgc gggcaatcgg
     2041 tgcctcgctg cgagctcgac gatcagggct cgatgaacga ggcccaatgc gctcgccacc
     2101 acaatgaggc ccccgttagg ctcgcgcgcg aatgcagcga cggagcgctc gatctcgccg
     2161 acatcgcgca ggccgacggg gcttagctcc accccgagtg acccggcacc ggcctgaatt
     2221 gcgcccaact ggccgacgcc gccgatattc gccggacgaa ggaccgctac tcgggcgacg
     2281 cccggcgcta tctgcttgag cagctccagc catttggcgc ttgtgccgta ttccaatcca
     2341 gtaaaacccg tggcattgcc gcccggccgc gccaggctcg cgaccaggcc ggccgcgacc
     2401 ggatcgttca ccgaaaggaa cacgaccggc acagtgggag ccgcctgttg cacagcggcc
     2461 gcatgcgcac cgccagaagc gatgatcacg tccggcgaaa gcgctgagat ttccgccgcg
     2521 tatctgcgaa tgcggtcggc ttcgcctgcg ctccaacgat actcgatccg caagttgcgg
     2581 ccgatcgtcc agcccagttc ctgcagcccc tgagccaacg ccgcgacgcg ggccggcgcg
     2641 tccgcatcgt ccgcagcgat gttcatgagt acgccgaccc gccgcacccg ctcgccttgc
     2701 tgcgcgcgcg ccgtgagcgg ccacgccgct gccgcgctgc cgagcagcgt gatgaagtgg
     2761 cgacgtttca ctgcccgata cctcccgtgc ttccgacaga aaggatagca c
//
LOCUS       DATLJT010000860         3083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1204565,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000860 DATLJT010000000
VERSION     DATLJT010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204565"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..722
                     /locus_tag="VM910_19725"
                     /pseudo
     CDS             1..722
                     /locus_tag="VM910_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
     gene            complement(863..1270)
                     /locus_tag="VM910_19730"
     CDS             complement(863..1270)
                     /locus_tag="VM910_19730"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI64792.1"
                     /translation="MDRCAPQKQVWCAQIILATADGCGTAEIMRQSGKAKPVVWRWQA
                     RFMEEGVDGLLRDKTRKQGKPPLPTATVQKVIDLVTGPPPGETTHWTGRMLATAVGIS
                     FLARCRARVLAKNIRPSSYLANPVEQEPTSAGG"
     gene            1846..2100
                     /locus_tag="VM910_19735"
                     /pseudo
     CDS             1846..2100
                     /locus_tag="VM910_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            2323..2583
                     /locus_tag="VM910_19740"
     CDS             2323..2583
                     /locus_tag="VM910_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64793.1"
                     /translation="MLNEEEEWIAEFEAVGESELRDRLYRGSGIHPEAKFHTAVRWLR
                     EQARARRLREEQLHRYVWWTLCAAVLSVIVGVIGVAVTWFGR"
     gene            2785..>3083
                     /locus_tag="VM910_19745"
     CDS             2785..>3083
                     /locus_tag="VM910_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64794.1"
                     /translation="MQTDKIVMGQMHGSRRLSAFLKSGDRRILIHRKVLTLNEEWSVS
                     MIEVDTGQLKRAVEAQHACTATLIQSVPVKETFEDETVWEGIVHVFKINGHPRAR"
BASE COUNT          609 a          923 c          944 g          607 t
ORIGIN      
        1 ttcggcaact gtgtcgcctt agcgcggttc aagcggcgag cgtccgaatt gcggtttgaa
       61 agtaggattc gtgtggatca ttacccctgg aatgtgccgg attttcaaac cgacccgcaa
      121 gctaagacct atccgttcgc ctacagcgcc gacgagattc cagatttggc gccttcgatt
      181 gctcgtggct atcccgatcc ggcggtgaga tagaccggtg ggtacgcaag ttcttgcggc
      241 cagggcgccc gacaccgaca ggaaggctgt tgatgactct tacacatggc ataaaggagg
      301 ggttcaccta taccaggcgc gccgaacacg gcactcagga gccagttgca acagtgagga
      361 ttggacgtgg cagttgccgc gactttgcgt tattgatgat agaatcggtc cgctcgctcg
      421 gtctcgcggc ccgtttcgtt tcgggctatc tgcatgttcc ttcgcgtgat ggtcccgaat
      481 atgtcggcgg tggctcgacc cacgcgtggt gccaagtcta tctgccgggc gccggctggg
      541 tcgagtttga tccgacaaac ggcatcattg gcaatcgtga cctcatccgc gtcgctgtcg
      601 cgcgcgaccc caggcaagca gttccgctct ccggcaccta cgtcggaaat tcggaggacg
      661 agttaggtat gaaggtcgaa gtcagcgtaa aatgtgagtc tgatccccgt aatcgtcaat
      721 agcagcggca catcacttgg gctcatctca tgccggctcc tgatggtgct gtcagcacgg
      781 ggcctggcca tcatggggtt ggggccggca cttctcccgg gcgcgcgaaa cggtagcacg
      841 ctgcaaaccg acgcatccgt cactatccgc ccgctgatgt tggctcttgc tcaacggggt
      901 tagccaaata tgagctggga cggatgtttt tcgccaaaac gcgagctctg cagcgtgcta
      961 ggaagctgat ccctaccgcc gtggccagca tccgtcccgt ccaatgagtg gtttctcccg
     1021 gcggcggccc ggtcacgagg tcgataacct tctggacggt cgcggtcggc agcggtggct
     1081 tgccctgttt gcgcgttttg tcgcgcagca gcccatcaac gccctcctcc ataaaccgcg
     1141 cctgccagcg ccacaccacc ggcttggctt tgccagactg gcgcatgatc tcggcggtcc
     1201 cgcagccatc agcggtggca aggatgatct gggcgcacca cacctgcttc tgcggcgcgc
     1261 agcgatccac gacgatcgcc tcaagccgat ggcggtccac cggagtgacg ttgacgacaa
     1321 ttcctgctct catgaggaga gcttgcactg cttcgggcga tttgggaatc gcccgaatga
     1381 atcagaccac taggtgggag ccttaaatcg gacgcgcgcg acccaagagc gagggtagcg
     1441 tgagggtggc ggcggtgcga tttccatacc tcaggtcgcg acggagtcgg tgcgcgccaa
     1501 tgtaacccat tccatagtat gaattagttg cggggtaacc ggccgtgact gaacgtccat
     1561 tcaagacgat caagctgaaa aagaaccttg cgggctcggc gctgatattt ccttctgaga
     1621 tcagaacggt ccaggcggcg attgtattcg taaagtcact tgagcccaaa acgcgggcca
     1681 aattgcattg gcgattggcc caatcggccc tagctgcact tgatactagg cctgttgggc
     1741 agggtactta cgaccgtgcg aacagggcaa tgcgccacgc tctggacgcc gaacgctggc
     1801 tggccgactg agtgactcta tgcgcagcac cgtgaggagg aaaaaatgac cggtgtgccg
     1861 ccgccactct ccgctcgtga tcggctagaa gaggctctcg ctcgcatcgc cgacgcgaac
     1921 ggcgagggtt cccgcgcctg cctcactgtt tattcgcaag tcgcccgcaa cgccgccgac
     1981 gcggccgacg cgggagcgcg tgccggtatg actctcggtc cgctcgatgg aacgatcgtt
     2041 agcatcaagg acctgttcga tgtcgccggc gaggccacgc gcgcggggtc caaggtgctc
     2101 gtcgatgcac cgcccggcgc aagcgacgcg ttgccattgc cgcgcgcgcg cgccctgccg
     2161 gttgggctga tgttagttgc gcgcaatggc cacgatcgca ggttgtttcg aatggcggct
     2221 gcggtggaac gccttttttc agcgtgacag atgtggttct catacgacca cgacagcgga
     2281 gcgccttgct tgctccagcg tcgccgcaaa ctcgggtgac caatgctgaa cgaagaggaa
     2341 gagtggatcg ccgagttcga agccgtcggg gagagcgaac tgcgcgatcg gctctatcgc
     2401 ggctccggca tccatccaga ggcaaaattt cacacggcgg ttcgttggct gcgcgagcag
     2461 gcaagagcgc gcagactccg agaagaacag ctccaccgct acgtttggtg gacgttatgc
     2521 gccgccgtcc tgtcggtgat cgtaggcgtc atcggagtgg ccgtcacatg gttcggacgc
     2581 taaattaaat agtaactcct gacgatcacc gtcgcccgct gccgcgacac ggcgaacaag
     2641 cgccgatgcc aacatcccac ccacgccgaa ggcgcagccc caaacccgac cgccgccggc
     2701 gcaccggagc tgctcgcttc ctgcccgcac agctgcgccg aggcgctccc ccgccggcat
     2761 agctggtgtc acgcgcaaaa tcttgtgcag acggacaaga ttgtaatggg ccaaatgcat
     2821 ggcagccgcc gcctaagcgc cttcctgaaa tcgggtgatc ggcgcatact cattcatcgt
     2881 aaggttttga cgctcaacga agagtggtcg gtttccatga tcgaggttga cactggtcaa
     2941 ttgaagcgtg cagtcgaagc tcagcacgcc tgcacggcga cgctaattca atcagtgccg
     3001 gtcaaggaaa cattcgagga cgaaacagtc tgggaaggca tcgtgcacgt cttcaaaatt
     3061 aacggccatc ccagggcgag gat
//
LOCUS       DATLJT010000861         4799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1204748,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000861 DATLJT010000000
VERSION     DATLJT010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204748"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /gene="asnB"
                     /locus_tag="VM910_19750"
     CDS             <1..1612
                     /gene="asnB"
                     /locus_tag="VM910_19750"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HVI64795.1"
                     /translation="LEACALWGVEAAIERAVGMFAFALWDRKTRSLTLARDRLGIKPL
                     YYAATPERVIFASQLKAFRAAPDWRPTIDDNAVVGYLRHAYIAQPRTIYREAEKLAPG
                     HILTLRKGMAPVPKCFWDLRGIAVAGQRRNDPAPDANEAVERLDALLRDSVGLRMIAD
                     VPLGAFLSGGIDSSTVVALMQAQSSRPVKTFSIGFHESGYDEAQCAKQVAAHLGTDHT
                     EFYVEPRHALDVIPHLADWFDEPFADSSQIPTYLVSELTRKHVTVALSGDGGDELFAG
                     YNRYLWTERVARAMKLMPRPLRGASAAALRSLAPQTWNRLFAFVPASWRPALPGDKLH
                     KITTLLDNPEPDAIYRRLVSQWERPDEVAATGLEPRGPLWDPSIARDFPDLVPRMQFL
                     DMISYLPEDILTKVDRATMAVGLEGRVPLLDHRVVAYSWSLPLAFKVRGGRGKWLLRR
                     VLDRYVPRSLIDRPKMGFGVPIDAWLRGPLREWAESLLAPARLASDGFVRVAPVRQAW
                     QEHLEGTRNWQYPLWTVLMLQAWRERWA"
     gene            1609..2712
                     /locus_tag="VM910_19755"
     CDS             1609..2712
                     /locus_tag="VM910_19755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI64796.1"
                     /translation="MTLRPRKVLYVTAGLRGGGAEAMLTRLASAQPGVADDITVVSLL
                     PAEAHMERLGAGGVTVVELRFDRAGGIATGLFKLASLIAESRPDIVQGWMYHGDLAAL
                     VALVLSGRRRQTRLVWSIRCTAMDWRGYGVGLWLVVKACTLLSRWPDLVTANSVAGLK
                     SHLALGYRPRRAEVVANGIDIDEFRPDSAARHAVRTELGIADDAIVLAHVARVDAMKD
                     HGSFLAAMAELPDLSAVLIGAGTENLPAASNVLRLGRRHDVARLFAAADFAVSSSRCG
                     EGFSNVLAEGMACGLPAVATDVGDAKLIVGDTGLVVPPESPDALAAAIRTLAAESAAA
                     RAERGRKARARIAENFAMPRAVQRYVDLYESLG"
     gene            2770..3402
                     /locus_tag="VM910_19760"
     CDS             2770..3402
                     /locus_tag="VM910_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVI64797.1"
                     /translation="MWQEVATISPARAVGRMNHAETFFAAAAQICHDISFASIEALAS
                     ELDRLRDRKGRLFLIGVGGSAANCSHAVNDFRKLCGIETYSPVDNVSELTARTNDEGW
                     ETVFAAWLDTSHLCDRDAILIYSVGGGDAERNISANIVRAIDLAKARSAKVFGIVGRD
                     SGYTAKHGDVVVLIPQVNPNWITPLSEAFQAVVWHCLVSHPALQRRQTKW"
     gene            3396..3965
                     /locus_tag="VM910_19765"
     CDS             3396..3965
                     /locus_tag="VM910_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI64798.1"
                     /translation="MVSRAHGLRPAVFLDRDGVIVVPEFRDGRSFAPRRLEDFRLYPD
                     AAASLQKLKQAGFLLAVVTNQPDVGDGLITHSEVDAMHEIMARELPLDAVKACFHGHG
                     ERCDCRKPKPGMILEAAVELGINLKKSFMVGDRKSDVEAGRAAGCATVFIDLGYALPA
                     PDAPDYVVHSIAEAANVIIETILTAQEGP"
     gene            3962..4702
                     /locus_tag="VM910_19770"
     CDS             3962..4702
                     /locus_tag="VM910_19770"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVI64799.1"
                     /translation="MSRVADLKVKIFADGADYEGIVKMSKNPVIKGFTTNPTLMRKAG
                     VSDYEAFARKVLAAIPDRPVSFEVFADDFASMAEQARTIAAWGPNVNVKIPVTNTKGQ
                     SASELIRALSSEGVTLNVTAIFTLDQVRTVVDALHPATAAIVSVFAGRIADTGVDPIP
                     HMRACKQITASRPKAELLWASTRELLNIFHAEQSGCDIVTVPNEFLSKLDLVGKDLAE
                     YSRETVQSFYRDATAASYQIKTTRFAAE"
BASE COUNT          827 a         1515 c         1547 g          910 t
ORIGIN      
        1 gctcgaagcc tgcgctttat ggggcgtcga ggctgcgatc gagcgcgcgg tcggcatgtt
       61 tgcgttcgcg ctgtgggacc gcaagacgcg cagcttgacg ctggcgcgcg accgcctcgg
      121 tatcaagccg ctctactacg cggcgacgcc ggagcgagtc atcttcgcct cgcagctcaa
      181 ggcgtttcgt gccgcaccgg attggagacc gacgatcgat gacaatgcag tcgtcggcta
      241 tctccgccac gcctatatcg cccagccgcg cacgatctat cgcgaagccg aaaaactcgc
      301 ccccggccac atccttacgc tgcgtaaagg catggcgccg gtgccgaaat gtttctggga
      361 tctgcgaggg atcgcggtcg cggggcagcg gcgcaacgat cccgcgccgg atgcaaacga
      421 ggccgtcgag cgcctcgacg cgcttctgcg cgactccgtc ggcctacgga tgattgctga
      481 cgtcccgctc ggcgcattcc tgtccggggg gatcgattct tcgaccgtgg tagcgctgat
      541 gcaggcgcaa agctcgcggc cggtgaagac gttctccatc ggctttcacg aaagcggcta
      601 tgatgaagcc cagtgtgcga agcaggtcgc cgcccatctc ggcaccgacc ataccgaatt
      661 ttatgtcgag ccgcgacatg cgcttgacgt cattccgcac ctcgcggact ggttcgacga
      721 gccgttcgcc gattcctcgc agatcccgac ctacctcgtc tccgaattga cccgcaagca
      781 cgtgaccgtg gcgttgtcgg gcgacggtgg ggatgagctg ttcgccggct ataaccgcta
      841 cctctggacg gagcgggtcg cgcgcgcgat gaagctcatg ccgcgtccgc tgcgcggcgc
      901 gtcggcggcg gccttgcgtt cgctcgcccc gcaaacctgg aaccggttat ttgcgttcgt
      961 gccggcctca tggcggccgg ccctgcctgg cgacaagctg cacaagatca cgacgctcct
     1021 tgacaatccg gagcccgacg cgatctaccg ccgcctcgtg agccaatggg agcggcccga
     1081 cgaggtcgcc gccacgggct tggagccgcg cggaccgctg tgggacccga gcatcgcccg
     1141 cgatttcccc gatctcgttc cgcgcatgca attcctcgac atgatcagct atcttcccga
     1201 ggacattctg accaaggtcg accgagcgac gatggccgtg gggctcgagg gccgcgtgcc
     1261 gttgctggat caccgcgtcg ttgcttactc ctggagcttg ccactcgcct tcaaggtgcg
     1321 cggcgggcgc ggcaaatggt tgctgcgccg cgtgctcgat cgctacgtgc cgcgctcgct
     1381 gatcgaccgg ccgaagatgg gtttcggcgt gccgatcgat gcctggctgc gaggtccttt
     1441 acgggaatgg gcggaatcgc tgctcgctcc ggcgcggctt gcgtctgatg ggttcgtgcg
     1501 ggtcgcgccg gtgcgccagg cgtggcagga acatctcgaa ggcactcgca actggcagta
     1561 tccgttgtgg accgtgctga tgctgcaggc gtggcgcgag aggtgggcgt gaccctgcgg
     1621 ccgcgcaagg tactctatgt cacggcgggt cttcgaggcg gcggtgccga ggcaatgctg
     1681 acgcggcttg cctcggctca gcccggcgtc gccgacgaca tcaccgtcgt gagtcttctc
     1741 cccgccgaag cccatatgga acggctgggc gcggggggcg tcacggtcgt cgagctgcga
     1801 ttcgacagag ccggcggcat cgcgaccggt ctgttcaagc ttgccagcct catcgccgag
     1861 agccgacccg atatcgtgca gggctggatg tatcacggcg atctcgccgc gctcgtcgcg
     1921 ctcgtcctgt cgggacgccg cagacagacg cgtctcgttt ggagcattcg ctgcacggcg
     1981 atggattggc gcggctatgg cgtcgggctg tggctggtgg tgaaagcctg cacgctgctg
     2041 tcgcgatggc ccgatctggt gacggccaac tcggttgccg gtctcaaatc gcatttggcg
     2101 ctgggctatc gcccgcggcg agcggaggtc gtcgctaacg gcatcgacat cgacgagttt
     2161 cggcccgact ccgccgcgcg gcacgcggtg cgcaccgagc tcggcattgc cgacgatgca
     2221 atcgtgcttg ctcacgtggc gcgtgtcgac gcgatgaagg atcacggcag tttcctcgct
     2281 gcgatggcgg agctgcccga cttgtctgca gtgctgatcg gagccggcac ggaaaatctt
     2341 cccgccgcgt ccaacgtcct tcgactcggc cgccgccacg acgtggcgcg tttgttcgcc
     2401 gccgcggatt tcgcggtatc gagttcgcgc tgcggcgagg gcttttcgaa tgtccttgcc
     2461 gaaggaatgg cctgcgggct ccccgcagtc gcaaccgatg tcggcgatgc gaagctcatc
     2521 gtcggcgaca cgggcctcgt cgtgccgccc gaaagtcctg atgcgctcgc tgcggcgatc
     2581 cggacattgg ccgccgaatc agcagcggcg cgggcggagc gcgggaggaa ggctcgtgcg
     2641 cggattgcgg agaattttgc gatgccacgc gccgtccagc gctatgtcga cctttacgaa
     2701 tcgctcggct gatcccgcca ttgatggctg gtagtttcca tcgacaaggc acttcaaatg
     2761 gaggggcgga tgtggcaaga ggtcgcgaca atttcacctg cgagggcggt tgggcggatg
     2821 aaccacgcgg aaaccttctt tgctgccgcc gcgcagattt gtcatgatat ttcattcgct
     2881 tcgatcgagg ctctggcatc cgagctcgac cggctgcggg accgcaaggg gcggctgttc
     2941 ctgatcggcg tcggcggcag cgccgccaat tgcagccacg ccgtcaatga ctttcgcaag
     3001 ctctgcggga tcgaaaccta cagccccgtc gacaacgtct cggagctcac cgcacgcacc
     3061 aatgacgagg gatgggaaac ggtgtttgca gcatggctcg acaccagtca cctctgcgat
     3121 agggatgcaa ttctgatcta ttcggtcggc ggcggcgatg ccgagcgcaa tatcagtgcc
     3181 aacattgttc gagcgattga tctggccaag gcgcggtcgg caaaggtgtt tgggatcgtg
     3241 ggccgggaca gcggatatac cgccaagcac ggcgatgtgg tcgtcctgat cccccaagtc
     3301 aatccgaact ggatcacccc cttgtccgag gccttccagg ccgtggtctg gcactgcctc
     3361 gtcagccatc cagccttgca gcgtaggcag acgaaatggt aagtcgtgcc cacggattga
     3421 gaccggccgt gtttctcgat cgggatgggg taatcgttgt tcctgaattc cgtgacgggc
     3481 gcagcttcgc cccgagacgg ctggaggatt tcaggctgta tcccgacgcc gcggccagcc
     3541 tccaaaagct gaagcaggcg ggttttctct tggcggtagt caccaaccag ccggacgtgg
     3601 gagacggctt gatcacccac tcggaagtgg acgcgatgca cgagatcatg gcgcgtgaat
     3661 tgcccctcga tgccgtcaag gcctgcttcc acgggcatgg cgagcgctgc gactgccgca
     3721 agccgaagcc aggcatgatc ctcgaggcgg ccgtggaact tggcattaac ctcaagaaga
     3781 gcttcatggt gggtgaccgc aagagcgatg tcgaggcggg ccgtgcggcc gggtgcgcca
     3841 ccgtgttcat cgatctcggc tatgccttgc ccgcgcccga tgctcccgac tatgtcgtgc
     3901 attcgatcgc cgaagccgcc aacgtcatca tcgaaaccat tctaaccgcg caggaggggc
     3961 catgagccgg gtcgccgatc tcaaggtcaa gattttcgcc gacggtgccg actatgaggg
     4021 catcgtcaaa atgtcgaaaa accccgtgat caagggcttc accaccaatc cgacgctgat
     4081 gcgaaaggcc ggggtcagcg attacgaggc attcgcgcgc aaggttctgg ccgcgatccc
     4141 ggaccgaccc gtgtcgttcg aggtctttgc cgacgacttc gcgagcatgg ccgagcaggc
     4201 gcgtacgatc gcggcatggg ggccgaacgt caacgtgaaa attccggtca ccaacaccaa
     4261 ggggcagtct gcgagcgagc tcatccgcgc gctgtcgtcc gaaggcgtga cgctcaacgt
     4321 caccgcgatc ttcacgctcg atcaggtccg caccgttgtc gacgcgcttc atccggcaac
     4381 agcggcgatt gtctcggttt tcgccgggcg catcgccgat actggcgtcg atccgatccc
     4441 gcatatgcgt gcgtgcaagc aaattactgc ctcgcgcccc aaggcggaat tgctttgggc
     4501 cagtacccgc gagcttttga acatcttcca tgccgagcag agcggctgcg acatcgtcac
     4561 cgtaccgaac gagttcctca gcaagctcga tctcgtcggc aaggatctgg cggaatattc
     4621 tcgcgaaacc gtgcaatcct tctatcgcga cgccacggct gcctcctatc agatcaagac
     4681 cacccgcttc gccgccgagt aagacggagg gaacgacaac catcaagatt ttttgctcat
     4741 gatccgagcc ggcgctaggc gttgaccttc aacagtgaag ggttctgtga aatcaaacc
//
LOCUS       DATLJT010000862        10647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_973461,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000862 DATLJT010000000
VERSION     DATLJT010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973461"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VM910_19775"
     CDS             <1..301
                     /locus_tag="VM910_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HVI64800.1"
                     /translation="HVIHAVGPVWNGGAKGEDELLASCYRRAIELYRANELSSIAFPA
                     ISTGVYRFPADRAARIAVSTTVDALSGASLSRVVFCCFSSESARLHEQAMTALGA"
     gene            444..1733
                     /locus_tag="VM910_19780"
     CDS             444..1733
                     /locus_tag="VM910_19780"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI64801.1"
                     /translation="MKPIHSVRPLISGALLAAATAASPAFAEGTFNIPPGAHFNPQKL
                     EKVGDYLRDQIANGKIPGAIVLIQQHGKPVYHEFFGVQDVASKQPMTDQTIFRLFSMT
                     KAITGVAAMILLDEGKFRLDDPVAKYIPAFANVKVGVEKTNEDGSKALELVPPRRPPT
                     VLDLMRHTSGITYGFYGDSLVRKAYAAANIYAGDFDLAEFAERIARLPLQDQPGTLWE
                     YGHSTDILARVMEVTSGKSLLQIEREKLLDPLGMNDTRFFVTDPEKQKRMAQPMPNDS
                     DFRVGRDNNPTVAKKWESASGGMVSTMADFARFAQMLLDGGKFDGKTYLSEKGFALLT
                     TDQVGPGADIGRDFFYFPGDGFGFGLGLAVRTDPGNARPPPPGSLGEVKWDGASGCYF
                     VVDRKQDMFFVLLEQTPSERQKIQPAVKKLIYEALEN"
     gene            1714..3006
                     /locus_tag="VM910_19785"
     CDS             1714..3006
                     /locus_tag="VM910_19785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI64802.1"
                     /translation="MKLWRTDRRAARVFVAALAILLGAAKPDLACAQSAVSPARSFST
                     GGLTRVAGYIANEIRSGKIPGAILLIQQHGKPVVFENFGVRDVATELSMTADTIFRLY
                     SMSKPITSVAAMMLVEDGKLRLEDPVSKFIPAFANMKVGVEKKDEGSKVTLALEPLKR
                     PITIEDLLRHTSGITYGFDSNNPVRQLYAAEDLFNADLTNAELVERITKLPLAEQPGT
                     VWDYGHSTDVLGRVIEVVSGKTLFQFEKERLLDPLGMTETTFFVADPAKRPRIAEPMP
                     NDRSISPVTSIRDPMQPRKAESGGAGMVGTIDDYARFAQMLLNGGTLDGRRYLRPETI
                     ALMTSDHIGPETHIAHDSFYYPGETRGFGLGFAVRISVWPNTQWPLGEYGWDGVAGTF
                     FFIDPVDDMFVIFMMQTPSERGRIQLELKKLIYAAMGR"
     gene            complement(3073..4140)
                     /locus_tag="VM910_19790"
     CDS             complement(3073..4140)
                     /locus_tag="VM910_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HVI64803.1"
                     /translation="MFYTPPPRDPKAPPVRINLLSDTQTRPTAGMRDAMARAEVGDEQ
                     IGDDPTVNLLCERVADLLGKEAAVFMPSGTMCNVAATLVHCRPGDEILAHETAHVIAR
                     EGGAHAALGGFQIMPLKGDDGQFSPATLRAALHPRTRYQPPQTLVSVEQTANIGGGTI
                     WKKAALDEVVAIARSAGLATHMDGARLLNATVATGISARDMAAGWDSAWIDFSKGLGA
                     PIGGVIAGSRAFIDEVWRWKQRLGGSMRQSGICAAACVYALDHHVDRLAEDHANARVL
                     ARGLSQIAGIDVQEPETNLVFFKPDGAGISGDKMVAALRRRGVLLAMMDGRIRVCTHL
                     DVTAAMIEETIGHVRDIVRGT"
     gene            complement(4310..4927)
                     /gene="rpsD"
                     /locus_tag="VM910_19795"
     CDS             complement(4310..4927)
                     /gene="rpsD"
                     /locus_tag="VM910_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HVI64804.1"
                     /translation="MTKRSEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGK
                     ISDFGVQLRAKQKLKGYYANISERQFHGIYVEAGRMKGDTGENLIGLLERRLDTVVFR
                     AKFVSTMFAARQFINHGHIKVNGRRVNIGSYQLKVGDTIEVKEASKQLVHVLEAAQLP
                     ERDVPDYIEADHSKMTAKFGRVPGLSDVPFPVQMEPHLIVEFYSR"
     gene            complement(5207..5974)
                     /locus_tag="VM910_19800"
     CDS             complement(5207..5974)
                     /locus_tag="VM910_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVI64805.1"
                     /translation="MPTSLNRLREEWRQKRPTFGAIATILSIQTVQIMARSGLDWIIV
                     DLEHGPIDLTSAHAMITATSGTPCVPFARIAANEAHLAKAPMDIGALGINFPMISSRA
                     EAEKAVRCLRYPPRGDRLWGPFHAPFRWGVSMQDYMATADDDMICMITIEHVEAIDRI
                     DEIMATPGIDVAVIGPGDLATSINKRGQIDDPEVQALIARAEAGILNSGVPIGGVART
                     AEQANAMIDRGYLALALGFDWSLFQRGLAAAFQGIKR"
     gene            complement(5986..6783)
                     /gene="murI"
                     /locus_tag="VM910_19805"
     CDS             complement(5986..6783)
                     /gene="murI"
                     /locus_tag="VM910_19805"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HVI64806.1"
                     /translation="MSRPPSILVFDSGLGGLTVLREIVATRPDAHYVYVADDAFFPYG
                     HHSEQQIIARVVPLIGELIDAHSSDLVVIACNTASTLVMSHLRSAYKTPFVGTVPAIK
                     PACAQSKTKRVSVFGTKGTVQREYTKALIRDFAQGCEVTLVGSPELASLAESAFNGQI
                     VSDAHIAAELVPCFVGDAEDATARTDTVVLACTHYPLLLDRLVRLAPWPVEWIDPAPA
                     IARRVSDLLGPPGVEVDKAGAEMIFTSKRPHALGRALMPFFGGRVPA"
     gene            6948..7499
                     /locus_tag="VM910_19810"
     CDS             6948..7499
                     /locus_tag="VM910_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HVI64807.1"
                     /translation="MLENTSNNWGSISRWFHWILGAAIIGMIAYGWWMNHFPARADRF
                     FYRSIHADIGYVILLLTVIRLVWRGINPTPELPGDTPRWQRLAARISHWALYLTTILV
                     AMLGWAMSGARTQNYASWFGLFNVPQFTSPDKAAANAYEERHILFAYVLLALIVLHVA
                     AAAWHHFLRRDGVALRMVKGEPG"
     gene            complement(7513..8820)
                     /gene="purB"
                     /locus_tag="VM910_19815"
     CDS             complement(7513..8820)
                     /gene="purB"
                     /locus_tag="VM910_19815"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVI64808.1"
                     /translation="MIPRYTRPEMAAIWEPQTRFKIWFEIEAHAADALAELGVIPKEA
                     AATIWAKGRNATFDVARIDEIERETKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLD
                     TCLNVQLARAADLLIEDIDKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
                     YAEFSRACERLKLARMEIATCAISGAVGTYAQIDPRVEEHVAKAMGLVPEPISTQVIP
                     RDRHAMYFATLGVVASSVERLATEIRHLQRTEVLEAEEFFSEGQKGSSSMPHKRNPVL
                     SENLTGLSRMVRAYVTPALENVVLWHERDISHSSAERMIGPDATVTLDFALNRLAGLI
                     DKLLIYPANMEKNLDRLGGLVHSQRLLIALTQKGASREDAYSLVQRNAMPVWRGEGDF
                     RTLLKKDADVKKYLSDAEIDEQFDLAYHLKHVDTIFRRVFGEA"
     gene            9012..9752
                     /locus_tag="VM910_19820"
     CDS             9012..9752
                     /locus_tag="VM910_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HVI64809.1"
                     /translation="MLLAVFADIHANRPAFAACLEAARVRGAERIILIGDYVGYGADP
                     EWAVDTVARLVDDGAIAVRGNHDQAIGTPSETMNAEAQVAIEWTRGRLDVAQRQFLAN
                     LPMMLAEDDRLYVHSEASNPARWRYVRSTTDAARSIAATEARITFCGHIHRPALYSMS
                     VTAKMTSFVPTTGVPVQLLGGRHWLAVVGSVGQPRDGNPAASFATYDTASREITYCRV
                     PYDIEAAAKRILDNGLPVWLADRLLVGM"
     gene            9758..>10647
                     /locus_tag="VM910_19825"
     CDS             9758..>10647
                     /locus_tag="VM910_19825"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVI64810.1"
                     /translation="MTRFSIRTGAAIDGFTIGERVHNGGMATLWSVTRPDIAAPLLMK
                     IPRVSEGEDPAAIVSFEMEQMILPRLTGPHVPTTFGTGDFSRQAYVVMECIPGDTLYK
                     RLPSLPLPYEEARALVGKIAVALADLHRQDVIHHDIKPSSIMFRDSGEAVLIDYGLSH
                     HNHLPDLLQEEFRVPFGTAPYMAPERLLGVRDDPRSDLFSLGVLLYFFTTGVRPFGET
                     ETLRGMRRRLWRDPHPPRKLKPDYPPFLQEIVLRCLEIQPVWRYPTASQLAFDLGHPD
                     QVKLTARSEKIKRDPLSTVWR"
BASE COUNT         1976 a         3306 c         3327 g         2038 t
ORIGIN      
        1 gcacgtgatc cacgcggtcg gaccggtttg gaatggcggc gcaaaggggg aggacgagct
       61 gcttgcctcc tgttatcgcc gtgccatcga actctaccgg gcaaacgaac tctcctcgat
      121 cgcctttccc gcgatttcga ccggcgtcta ccggtttcct gccgaccgtg ccgcccgcat
      181 tgccgtgtcg accaccgttg acgcgctgtc cggcgcctct ctcagtcgtg tcgttttttg
      241 ctgtttctcc agcgagagcg cgcgcttgca tgaacaagcg atgacggcgc ttggagcatg
      301 atcaggaaaa gtgggcaccg gatttcctcg cgacaaacgc ggaacgcttt tgcagagatc
      361 atgctcaaac aataaatcta gcgctttggc aggccttgta ccgattgaac cggccgctac
      421 actctgccca aaagggaggg tatatgaagc caattcattc ggtgcgcccg ctcatatccg
      481 gtgcgcttct cgcggccgcc acagcggctt cgccggcgtt cgccgaaggc actttcaaca
      541 ttccgccggg tgcccatttc aatccgcaga agctcgagaa ggttggcgac tatcttcgcg
      601 accagatcgc caacggcaag atccccggtg cgatcgtcct gatccagcaa cacggcaagc
      661 cggtctatca cgagttcttc ggcgttcagg atgtcgcctc caagcagccg atgacggatc
      721 aaacgatttt ccgtctgttc tcgatgacca aggccatcac gggcgtggcc gccatgatcc
      781 tcctcgatga gggcaagttc aggcttgatg atcccgtcgc gaaatacatc ccagcctttg
      841 ccaatgtgaa ggttggcgtg gagaagacga acgaggatgg cagcaaggcg ctggagctcg
      901 tccctccgcg gcggccgccg accgttctcg acctgatgcg tcacacctcc ggcatcactt
      961 atggtttcta cggcgacagc ctggtccgca aggcctatgc cgccgccaat atctatgcgg
     1021 gcgatttcga cctcgccgaa tttgcggagc gcatcgccag gctacctctc caagaccagc
     1081 cgggaacgct ctgggaatac ggccattcca ccgatatttt ggcgcgggtc atggaagtca
     1141 cgtccggaaa atcactgctg cagatcgaga gggaaaagct gctcgatccg ctcggaatga
     1201 acgatacgcg cttcttcgtg accgatcccg agaagcagaa gcggatggcg cagccgatgc
     1261 cgaacgacag tgatttccgg gtcggccgcg acaacaatcc gaccgttgcg aagaaatggg
     1321 aatccgccag cggcggcatg gtgtcgacca tggcggactt cgcccggttt gcgcagatgc
     1381 tgctcgatgg cggcaagttc gatggcaaga cctatctgag cgaaaagggg ttcgcgctgt
     1441 tgaccaccga tcaggtcggc ccgggcgccg acatcggccg cgacttcttt tatttcccgg
     1501 gggacggttt cggcttcgga ctcgggcttg cagtgcggac ggatcccggc aatgcgcggc
     1561 cgccgccgcc aggctcgctc ggcgaggtaa aatgggacgg tgccagcggc tgctatttcg
     1621 tcgtcgatcg caagcaggac atgttcttcg tgctgctgga gcagaccccg tcggagcgcc
     1681 aaaagatcca gccggcggtg aagaagctga tctatgaagc tctggagaac tgaccgccgg
     1741 gcggctcgcg tcttcgtcgc ggcgcttgca atcctgctcg gtgcggccaa gccggatttg
     1801 gcgtgtgcgc aatccgccgt gtcgccggcg cgcagctttt cgaccggggg actgacgcgc
     1861 gtcgccggct acattgccaa cgaaatccga agcggaaaga ttcccggcgc gatcctcctg
     1921 atccagcagc atggcaagcc ggtcgtcttc gagaatttcg gcgttcgcga cgtcgcaacc
     1981 gaactatcga tgaccgccga caccatcttt cgcctctatt cgatgtcgaa gccgatcacg
     2041 tcggttgcgg cgatgatgct ggtcgaggac ggcaagctca ggcttgagga tcccgtttca
     2101 aagttcatcc cggccttcgc caacatgaaa gtgggagtcg agaagaagga cgagggcagt
     2161 aaggtcacac ttgcactcga accgctgaag cggccgatca ccatcgaaga tttgttgcgc
     2221 cacacgtccg ggattaccta cggcttcgac agcaacaatc ccgtacgaca gctctacgca
     2281 gcggaagacc tcttcaacgc cgatctgaca aatgccgagc ttgtcgagcg catcaccaaa
     2341 ttgccgctcg cggaacagcc gggaacggtg tgggactacg gccattcgac cgacgtcctc
     2401 ggccgcgtca tcgaggttgt gtcgggaaag acgctgtttc aatttgaaaa ggaaaggctg
     2461 ctcgatccgc tcgggatgac cgagaccacc ttttttgttg cggacccggc gaagcggccg
     2521 cgcattgccg agccgatgcc caacgatcgc tcgatcagcc cggtcacgag cattcgcgac
     2581 ccgatgcagc cgcgaaaggc agaatccggc ggtgccggca tggtcggcac gatcgacgat
     2641 tacgcgcgct ttgcgcagat gctcctgaac ggtggtacgc ttgatggcag gcgatacctg
     2701 aggccggaga ccatcgcgct catgacctcc gaccatatcg gcccggaaac tcacatcgcc
     2761 catgattctt tctattatcc cggcgagacc cgcggtttcg gcctcggctt tgccgtgcgc
     2821 atctcagtgt ggccgaacac gcaatggccg ctcggcgaat atggctggga cggcgttgcc
     2881 ggcacgttct tcttcatcga tcccgtcgac gacatgttcg tgattttcat gatgcagaca
     2941 ccgtcggagc gcggtcggat tcaactcgag ttgaagaaac tgatttacgc agccatgggc
     3001 cggtgacggt gcgacggtgg gtacctccgt cgcgacgagt ggtttggcgc attggaagcg
     3061 acaattttgg gactaggtcc cgcgcacgat atcgcgcacg tggccgatcg tctcctcgat
     3121 catcgcagcc gtcacgtcga gatgggtgca cacgcggatg cggccgtcca tcatcgcgag
     3181 cagcacgcca cgccggcgca gcgccgcgac catcttgtcg cctgaaatgc ccgcgccatc
     3241 gggtttgaag aacacaagat tggtctcggg ctcctgcacg tcgatgcctg caatctgcga
     3301 cagtccgcgc gccagcaccc gcgcattggc gtggtcctcg gccaggcgat cgacatgatg
     3361 gtcgagcgca tatacgcagg ccgcagcgca aatcccggac tggcgcatcg agccgccaag
     3421 ccgctgcttc cagcgccaga cctcgtcgat gaatgcgcgg ctgcccgcga tcacgccgcc
     3481 gatcggcgcg cccaggccct tggagaaatc gatccaggcg gaatcccatc ccgccgccat
     3541 gtcgcgggcg gaaataccgg tcgcaacggt cgcattcaac agccgtgcgc cgtccatatg
     3601 ggtggcgagc cctgccgacc ttgcaatcgc cacaacctcg tcgagcgccg ctttcttcca
     3661 gattgtaccg ccgccaatat tggcggtttg ttcgacgctg acgagggttt gcggcggttg
     3721 atagcgtgtg cgcggatgca aggctgcacg caaggtcgcg ggcgaaaact ggccgtcatc
     3781 gccctttagc ggcatgatct ggaatccgcc gagcgccgca tgcgcgccgc cttcgcgcgc
     3841 gatgacgtgc gccgtctcgt gcgcgaggat ttcatcgccg ggtcggcaat gcaccaacgt
     3901 ggccgccacg ttgcacatgg tgccggaggg catgaacacg gctgcctcct tgccgagcag
     3961 gtcggcgacg cgctcacaca gcagattgac agtcgggtcg tcgccgatct gctcgtcgcc
     4021 gacctcggcg cgcgccatcg catcgcgcat gcccgcggtc ggacgggtct gcgtgtccga
     4081 caacagattg atgcgcaccg gtggcgcctt cggatcgcgc gggggagggg tgtagaacat
     4141 ggcagcctct tatcattgac catgtcattg ccggagttga cgcaatccat tttctgagga
     4201 agactctttt agcgatggat gcgcgggtca agcccacgat tgagcgaaat agccaaacaa
     4261 aaggccccgg caaaaccggg gccttcggca gctcgaaaag cgaaaccgct cagcgcgaat
     4321 agaattcgac gatcaggtgc ggctccatct gcaccgggaa aggcacgtcc gacaggcccg
     4381 ggacgcgccc aaacttggcg gtcatcttgg agtgatcggc ctcgatgtag tcgggcacgt
     4441 cgcgctccgg aagctgggcc gcctcgagga cgtgcacgag ctgcttcgag gcttccttga
     4501 cttcgatggt atcgccgacc ttgagctggt agctgccgat attgacgcgc cggccgttca
     4561 ccttgatatg gccgtgattg atgaactgac gcgcggcgaa catcgtcgac acaaatttcg
     4621 cccggaacac cacggtgtcg aggcgccgct cgagaagccc gatcaggttt tcaccggtat
     4681 cgcccttcat ccggccggcc tcgacataaa tgccgtggaa ctggcgttcg ctgatgttgg
     4741 cataatagcc tttcagcttc tgcttggcgc ggagctgcac gccgaagtcg gagatcttac
     4801 ccttgcggcg ctggccgtgc tggccgggtc cgtactccct gcggttcacg gggctcttcg
     4861 ggcggcccca gatattctgg cccatacggc gatcgatctt gtatttcgcc tcactgcgct
     4921 tagtcatcgc gtcctctttc aggtctagct agatttgagg agacgcgccc tcctgtgctt
     4981 cagggaaaat cctgcagccg acagatccaa ccgccaaagc ttgtaaaacg gacggaccac
     5041 gggtcgcgaa acgcttcgcg ggccgaaaac ggcccgcgag caaggcggct tttaggggaa
     5101 agtccgcggt tctgtcaagg caaaggtgac gcgaggcgtt gacgcatttt cgaaaataga
     5161 ttgcactatc aataacttat agtcgtaaat gtggatgctg cctgagctag cgttttatgc
     5221 cctggaaggc cgccgccaga ccgcgttgaa acagcgacca gtcgaagccg agcgcgagtg
     5281 caagatagcc acggtcgatc atcgcattcg cctgctcggc cgtgcgtgcc acgccgccga
     5341 tcggcacccc gctgttgagg atgccggctt cggcacgcgc gatcagtgcc tgcacttcgg
     5401 gatcatcgat ctggccgcgc ttattgatgg aggtggcgag atcgccgggg ccgatcacgg
     5461 caacgtcgat gccgggcgtc gccatgatct catcaatgcg gtcgatcgct tccacatgct
     5521 cgatcgtgat catgcagatc atgtcgtcgt cggcggttgc catgtagtcc tgcatcgaaa
     5581 cgccccagcg aaagggggcg tggaacggtc cccagagccg atcgccgcgc ggcggatagc
     5641 gcaggcagcg gaccgccttt tcggcttcag cgcggctgga aatcatcgga aaattaatgc
     5701 cgagcgcgcc gatatccatc ggtgcttttg ccagatgagc ctcattggct gcaatccgcg
     5761 caaatggcac gcagggcgtg cccgacgtgg cggtgatcat cgcatgtgcg gacgtcagat
     5821 cgatcgggcc gtgctcgagg tcgacgatga tccagtcgag cccggagcgc gccatgatct
     5881 gcacggtctg gatgctcagg atcgtcgcga tcgccccaaa ggtcggacgc ttctggcgcc
     5941 attcctcgcg caggcggttg agtgacgtcg gcattcacgc tcccatcagg ccgggacccg
     6001 gccgccaaaa aatggcatca gcgcgcgccc cagggcgtgc ggccgcttcg aggtgaagat
     6061 catttcggca ccggccttat cgacctcgac gccgggcggg cccagcaaat cagaaacacg
     6121 ccgcgcgatc gccggcgccg gatcgatcca ctcgaccggc cacggcgcaa gccgcaccag
     6181 ccggtcgagc agcagcggat aatgcgtaca ggccagcacc actgtgtcgg tgcgtgcggt
     6241 cgcgtcttca gcgtctccaa caaagcaggg cacaagctca gccgcgatat gggcgtcgct
     6301 gacgatctga ccgttaaagg cgctttcagc cagcgaagca agctcaggcg agcccaccag
     6361 cgtcacctcg cagccctgtg caaaatcgcg gatcagtgcc ttggtatatt cgcgctggac
     6421 ggtgcccttg gtgccgaaca ccgagacgcg cttggtcttc gactgcgcgc aggccggctt
     6481 gatcgcaggc accgtcccga caaaaggagt tttgtaggcg ctacgcagat gggacatgac
     6541 cagcgtggat gcggtgttgc aggcgatcac gaccagatcg gatgagtgtg catcgataag
     6601 ttcgccgatg agcggcacca cgcgggcgat gatctgttgc tcgctgtgat gaccataggg
     6661 gaaaaaggcg tcgtcggcga cgtagacgta atgggcgtct ggccgcgtgg ctacgatctc
     6721 acgcaacacc gtgagtccac caaggccgga atcgaacacc agaatcgaag gaggtcgtga
     6781 cacggcgcga tcttacccga tcccaagtta tcattcagtt gttttcaggg ccgtgagttt
     6841 caggtcgagg ggtgcgacga tggtgcaggc ggtttttgaa cgattcaagt cgcttcggct
     6901 tgaaccaccg gttaccagat gccgcagaca caggggattt gcaggacatg ctggaaaata
     6961 cgtcaaacaa ctggggcagc atttcaaggt ggtttcattg gatcctgggc gccgcgatca
     7021 tcggcatgat cgcctatggc tggtggatga accactttcc cgcgcgcgcg gatcgcttct
     7081 tctaccgatc gatccatgcc gatatcggtt acgtcatcct gctgctgacc gtgatccgcc
     7141 tggtctggcg cggcatcaac cccacgcccg aactgcctgg tgacacgccg cgctggcagc
     7201 gccttgcggc gcgcatcagc cattgggcgc tctatctcac gaccatcctg gtggcgatgc
     7261 tgggctgggc gatgtccggt gcgcgtacgc aaaattacgc gagctggttc ggcctgttca
     7321 acgtgccgca attcacctcg cccgataaag ccgcagccaa cgcttatgag gagcgccaca
     7381 tcctgtttgc ctatgttctg ctggcgctga tcgtgctgca tgtcgcggcc gcggcctggc
     7441 accatttcct caggcgcgac ggtgtcgcgt tgcgaatggt gaagggtgag ccgggatgag
     7501 cgtcggccgc cttcacgcct ccccgaacac gcgcctgaag atcgtatcga cgtgcttgag
     7561 gtggtaggcg agatcgaatt gctcgtcgat ctcggcatcg ctcagatact tcttcacgtc
     7621 ggcatctttc ttcaagagcg tgcgaaaatc gccttcgccg cgccagaccg gcatcgcatt
     7681 gcgttgcacg aggctgtagg cgtcctcgcg acttgcgccc ttctgcgtca gcgcgatcag
     7741 tagccgctgt gagtgcacga ggccgccgag ccgatcgaga ttcttctcca tgttggcggg
     7801 atagatcagg agcttgtcga tcagtccggc gagacgattg agcgcgaagt cgagcgtcac
     7861 ggttgcgtcg ggcccgatca tgcgttcggc ggaggagtgc gagatgtcgc gctcgtgcca
     7921 gagcaccacg ttctccagcg caggtgtcac ataggcgcgc accatgcgcg acaagccggt
     7981 gaggttttcc gacaacaccg ggttgcgctt gtgaggcatg gaggaagagc ctttttggcc
     8041 ctccgagaaa aattcctccg cctccagcac ctcggtgcgc tgcagatggc ggatctcggt
     8101 ggcgagccgc tcgacggaag aggcaaccac tccgagcgtc gcaaaataca tcgcgtggcg
     8161 gtcgcgcggg atgacctgcg tcgagatcgg ctcgggtaca agccccatcg ccttcgcgac
     8221 atgctcttcg acacgcgggt cgatctgcgc ataggtgccg acggcgcccg agatcgcgca
     8281 agtcgcgatc tccatgcgtg caagtttcag gcgctcgcag gcgcgcgaaa attccgcgta
     8341 ggcataggcg agtttcaggc caaacgtcac gggttcggcg tggatgccgt gcgagcggcc
     8401 gatggtcggc gtcatcttgt gttcgaaagc gcgcttcttc agcgctgcca gaaccttgtc
     8461 gatgtcctcg atcaacaggt cggcggcgcg ggcgagctgc acattcaggc aggtgtcgag
     8521 cacgtcggaa gaggtcatgc cctggtgcac gaagcgcgcc tcggggccga cgatctcggc
     8581 gaggtgggtg aggaaggcga tcacgtcgtg cttggtttcg cgctcgatct cgtcgatccg
     8641 cgccacgtcg aaggtcgcgt tgcgaccctt ggcccagatc gtcgcggcgg cctccttcgg
     8701 gatcacgccg agctcggcga gcgcatccgc cgcatgcgcc tcgatctcga accagatctt
     8761 gaagcgcgtc tgcggctccc agatcgcggc catttccggg cgggtgtagc gggggatcat
     8821 cgaaagctcc gaggcgagtt gggcgagcgt caggccggcg cctgccagat gtttttacgc
     8881 cgcgctttac cagatcgtcg tccccgacac tacccgcccg gggtggaaaa ccggccgtcg
     8941 atggcgcatc ctgttgatgt ccctcaaaac ctgcctcaca gcgggccata atctggggga
     9001 tggaggaacg cgtgctgctg gctgtttttg cggatatcca cgccaaccgg ccggccttcg
     9061 cggcctgcct ggaggcggcg cgtgtgcggg gagcggagcg aatcatcctt atcggcgact
     9121 atgtcggcta tggcgccgac ccggaatggg cagtcgacac ggtcgcaagg ctcgtcgacg
     9181 acggcgcgat cgccgtacgc ggcaaccacg accaggcgat cggcacaccc agcgaaacca
     9241 tgaatgccga ggcgcaggtc gcgatcgagt ggacgcgcgg ccggctggat gtggcgcagc
     9301 ggcagttttt agcgaacctt ccgatgatgc ttgcggagga cgaccgtctc tacgtccatt
     9361 cggaagcctc caatccggcg cgctggcgct atgtccgttc gaccacggac gccgcccgca
     9421 gcattgccgc aaccgaggcc agaatcacct tctgcggcca catccacagg ccggcgctct
     9481 actcgatgtc ggtcaccgcg aagatgacga gcttcgtgcc gaccaccggc gtgcccgtgc
     9541 agcttctggg aggccggcac tggctcgccg tcgtcggctc ggtcgggcaa ccgcgcgacg
     9601 gcaatccggc ggcgtcgttc gcgacctacg atacggcgtc gcgcgagatc acctattgcc
     9661 gcgtgcccta cgacatcgag gcggcagcga agcgtatcct cgacaacggc ttgccggtct
     9721 ggctcgccga tcggctattg gtcggaatgt gaaagcgatg acgcgattct cgattcggac
     9781 gggcgcagcc atcgatggtt tcaccatcgg cgagcgtgtc cacaacggcg gcatggcgac
     9841 cttgtggagc gtgacccgtc ccgatatcgc cgcgcccttg ctcatgaaga tcccgcgcgt
     9901 ctcggaaggc gaggatcctg ccgccatcgt ctccttcgag atggagcaga tgatcctgcc
     9961 gcgactaacc ggaccgcacg tgccgaccac tttcggcacc ggcgattttt ctcgtcaggc
    10021 ctatgtggtg atggaatgca ttccgggcga cacgctttac aagcgcctgc cgagcttgcc
    10081 gctgccatat gaggaggcgc gggcgcttgt cggcaagatt gcggtggcgc ttgccgacct
    10141 gcatcggcag gacgtcatcc atcacgacat caagccctcg agcatcatgt ttcgcgatag
    10201 cggcgaggcc gtgctgatcg attacggact gtcgcatcac aaccatctgc ccgatctttt
    10261 gcaggaggag ttccgcgtgc cctttggcac cgcgccctac atggcgcccg agcgcctgct
    10321 cggcgtgcga gacgatccgc ggagcgacct gttttcgctg ggcgtgctgc tctatttctt
    10381 caccaccggt gtgcggccgt tcggcgagac cgaaacgctg cgcggcatgc ggcggaggct
    10441 gtggcgcgat ccgcatccgc cccgcaagct gaagcccgat tatccgccct ttctgcagga
    10501 gatcgtgctg cgatgcctcg agatccagcc ggtctggcgc tacccgaccg cgtcgcagct
    10561 tgccttcgac ctcggccatc ccgaccaggt caagctgacg gcgcgctcgg agaagatcaa
    10621 gcgcgatccg ctctcgacgg tatggcg
//
LOCUS       DATLJT010000863         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2584383,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000863 DATLJT010000000
VERSION     DATLJT010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584383"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="glnA"
                     /locus_tag="VM910_19830"
     CDS             <1..757
                     /gene="glnA"
                     /locus_tag="VM910_19830"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642425.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HVI64811.1"
                     /translation="QHELGMKFDTMTLMADHLQIYKYCIHQVAHIYGKTATFMPKPVF
                     GDNGSGMHCHQSIWKEGKPVFAGNKYSDLSEACLSYIAGVIKHAKAINAFTNPTTNSY
                     KRLVPGYEAPVLLAYSARNRSAACRIPYTANPKAKRVEVRFPDPLANPYLGFAAMLMA
                     GLDGVKNKLDPGPAMDKDLYDLPKEELKTIPTVCGSLREALENLDKDRAFLKNGGVFD
                     DDFIDSYIELKMTEVERFEMTPHPVEFEMYYSG"
     gene            complement(840..>1590)
                     /locus_tag="VM910_19835"
     CDS             complement(840..>1590)
                     /locus_tag="VM910_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HVI64812.1"
                     /translation="VVGVIGNAYRIENRFTTQLVGERNLRAVADVAGALPLMFAGSPE
                     ITDVGALLDVVDGIILTGARANVHPTRFNTAPCEKHEPYDVSRDEVALALSEACVARG
                     VPLFGICRGLQEMNVAFGGSLHPEIRELPGRMNHRMPRLENGEIHPDPRVVFADRHEV
                     SLIPGGAFAKILGCEKIRVNSLHGQGILQPGKRVVVEGVAEDGTIEAIRIADAPGFAL
                     GVQWHAEYDAQRNPINRALFKAFGEALMVRV"
BASE COUNT          324 a          515 c          455 g          296 t
ORIGIN      
        1 ccagcacgaa ctcggcatga agttcgacac gatgacgctg atggccgacc atttgcagat
       61 ctacaaatac tgcatccacc aggtcgccca tatctacggc aagaccgcca ctttcatgcc
      121 gaagccggtg ttcggcgaca acggttcggg catgcactgc caccagtcga tctggaagga
      181 aggcaagccg gtgtttgccg gcaacaagta ctccgatctt tcggaggcct gcctgtccta
      241 catcgccggc gtcatcaagc atgcgaaagc gatcaacgcc ttcaccaatc cgacgaccaa
      301 ttcctacaag cgcctggtgc cgggttacga ggcccctgtg ctgctcgctt actccgcgcg
      361 caaccgctcg gccgcttgtc gcattcccta caccgccaac ccgaaggcca agcgcgtcga
      421 ggtgcgtttc cccgatccgc tggccaaccc ctatctcggc tttgccgcga tgctgatggc
      481 cggcctcgac ggcgtaaaga acaagctcga tccgggcccg gcgatggaca aggacctcta
      541 cgacctgccg aaggaagagc tcaagacgat cccgaccgtc tgcggttcac tgcgcgaagc
      601 gctggaaaat ctcgacaaag accgcgcgtt cctgaagaac ggcggcgtgt tcgacgacga
      661 cttcatcgac agctacatcg aactgaagat gaccgaagtc gaacgcttcg agatgacgcc
      721 gcatccggtc gagttcgaaa tgtattattc cggctgattt ggatcttcgg aacatcacgc
      781 tcagaactac gaaaggcgcc ctctcggggc gcctttttgt tgcggaattc tttagagcct
      841 tacacccgca ccatcagcgc ctcaccgaac gctttgaaca gcgcgcgatt gatcggattg
      901 cgctgcgcat catattcggc gtgccactgc acgccgagcg caaagccggg ggcgtctgcg
      961 atgcgaatcg cctcgatcgt gccgtcttcc gccacgcctt cgacgacgac gcgcttgccc
     1021 ggctgcaaaa tgccctggcc atgcagcgaa ttgacgcgga ttttctcgca gccgaggatt
     1081 ttcgcgaagg cgccgcccgg aatgaggctc acctcatgac gatcggcaaa gacgaccctt
     1141 ggatcggggt ggatctcgcc attttccagg cgcggcatgc ggtgattcat ccgtcccggc
     1201 agttcacgga tttccgggtg cagcgagccg ccgaaggcca cgttcatttc ctgcaggccg
     1261 cggcagatgc cgaacagcgg cacgcctcgc gccacgcagg cctccgagag cgccaacgca
     1321 acttcgtcgc gggagacgtc atacggctcg tgcttctcgc agggtgcggt gttgaagcgc
     1381 gtgggatgga cgttggcgcg cgcgcccgtc aggatgatgc cgtcgactac gtccagcaac
     1441 gcgccgacat ccgtgatttc gggagaaccg gcaaacatca gcggcagcgc acctgcaacg
     1501 tcggccaccg cgcgaagatt gcgctctccg accagttgcg tggtaaatcg attttcgatg
     1561 cgataggcgt ttccgatcac accgacgacc
//
LOCUS       DATLJT010000864         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3502932,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000864 DATLJT010000000
VERSION     DATLJT010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3502932"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..723)
                     /locus_tag="VM910_19840"
     CDS             complement(436..723)
                     /locus_tag="VM910_19840"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64813.1"
                     /translation="MSPPITMLLRPTVTFTPPAALGLALALSSAAAAQTTTFHDAAGR
                     VTGKATTRNGATTFYDSAGRVTGKTRTNAEGTTTFYDAAGHVTGKATAPRR"
     gene            951..2549
                     /locus_tag="VM910_19845"
     CDS             951..2549
                     /locus_tag="VM910_19845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64814.1"
                     /translation="MIRRVLSIGAAVAALSLLASPVVVAQERGGTLVMVVQPEPPTLA
                     SYLSTSGPIGQVTTKVFDGLLEYNFDLKPQPSLAESWAVSDDGNTITFKLRNDVKFHD
                     GHPMTSADVKFSIMEVLKKVHPRGANTFRELIDIETPDDYTAIFKLSNPAPYMLMALS
                     SYESPIIPQHVYGKGDIKQHENANKPIGSGPFKFVEWRRGQYMRFDRNPDYWKKGQPY
                     LDRIVARFIADSSTRTAAIESGEAHVAGFGALPYSDIKRLDALPQLAATTRGYEMQSP
                     IVQLDFNTRKPPFDKAKVRQAVSYVINRPFVIDNVWFGFGKPATGPISSNFAVTGMYT
                     DKVLRYDVPDAKERAEKLLDEAGYPRKADGTRFEITHDITPYGEEWQRFGEYTQQALA
                     AVGIKTTLRTEDVPTWLKRIYTDYDFNLTNNWIQTLADPVIGVHRLYHSNQIRPGTVF
                     VNGSGWSSPETDALMDKATVEVDAKKRNALYHELQTKLVEAAPYVWVHELQFVTVYNT
                     SFKDLIVSPLGLYTSFSQAYQAKK"
     gene            2631..3617
                     /locus_tag="VM910_19850"
     CDS             2631..3617
                     /locus_tag="VM910_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64815.1"
                     /translation="MTSPAVLLRYLVRRLAQAIPIVLGIAVLNFLLLQLAPGDAADVL
                     AGEAGSATPEYMAQLRERFGLDQPVYVQLMLYLKNVVTLDLGYSFRHGMPVLQLILGR
                     LWPTLLLMGTTLVLSVSVGVLLGLLAATRVHSWRDNLISILAIIAYATPLFWIGLMLI
                     VLFSINLGWFPASGMETVAAFYEGWDRAVDIAWHLVLPALTLSCFYLALYTRLMRATV
                     LEQQGMDYVTTAEAKGLTERQITFRHIQRNAILPVVTMVGIQIGSLLGGSVIVESVFG
                     WPGLGLLAYQSLFARDFNLLLGIFFFSACLVVIINLLVDLLYLALDPRIEIR"
     gene            3639..4466
                     /locus_tag="VM910_19855"
     CDS             3639..4466
                     /locus_tag="VM910_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64816.1"
                     /translation="MRGFWGRYARSRSGVLGLAILTTIILVALLAPVLFPYNPFQIVD
                     KPFLPPFGDYVLGTDSLGRNIAAGLAYGARTSLLIGVLATLLAVGSGVIVGGLAGYYG
                     GWLDDALMRVTEFFQTIPFFVFAIVLVAILSPSVQNIIIAIAIVTWPPVARLVRGEFM
                     AMRSRDFVSASVCLGTSDYDIIARDILPNCLSPIIVTGSLMVATAILIESALAFLGLG
                     DPNVMSWGFMIASGRTFLRSAWWLCAMPGTAILLAVLSINLVGEGLNDALNPRLRDV"
     gene            4469..6124
                     /locus_tag="VM910_19860"
     CDS             4469..6124
                     /locus_tag="VM910_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64817.1"
                     /translation="MAILKLRNLTVDLPPGADRAHAVEDVSLDVNSNEILCVVGESGS
                     GKSVMGRTVMGLLGRTRLKPSAGRVLFGEENLLTASPARMRQIRGDRISMVFQEPMTA
                     LNPLMTVGRQIDELLKIHRTMSHGDRKKRIHDLFASVLLPDPERIYASYPHQLSGGQR
                     QRAVIAMALILEPDVVIADEPTTALDVTTQAQILKLIKDLQAAHRTAFMFITHDFGVV
                     AEIADRVAVMQHGRVLETCTTANVLEHPKNDYTRTLLEAVPRLVPRAKCELTNPENVL
                     AVRDLSKTFGARRRFFRRARRGIEAVKSVSLALRRGETLGIVGESGSGKSTLARCVVR
                     LIESDAGEIRLGDIDFRALSRAQLRPYRKKIQMVFQDPYGSLNPRLTVGQLVAQGPIV
                     HGESCEAAMETVRELLRLVGLEPFVADRYPHEFSGGQRQRIGIARALALRPQILVADE
                     PVSALDVSVQAQVLRLLDNIRDRFNLAMLFITHDLRVAAQVCDRVAVMRYGQIVEEGP
                     TERLFAAPENDYTRELLAAVPGKQWEAIRDARRAQAAGVIQTI"
BASE COUNT         1083 a         1895 c         1901 g         1246 t
ORIGIN      
        1 gatcaggcta cacccctaac cagtgtacgt ggtagggggc cgggcgtgat ttttttctgc
       61 gcacactttg cgcttgaaat gacgcgatcg gcacggcgct ccacgccgag attctctaaa
      121 tcggctccat cgccccactg ggcagtcaag ctcgaccatt ttttcgttcg ctgagaggta
      181 gacgctgcct tgggaaaact ccttcaggac ccgacaccgt cggaagtctt cacgccaagc
      241 atctgcggcg ttggctaaat ggttgagaaa aaatgggcca tcagcaggta gagaaatccg
      301 aaggcgaact tcgtcatcgc ttgccccact cctcctccgc ttcgcgcacg gcgagcccag
      361 gcgcttcgac acgatgccct cggagcccag ccggcaggcg tggcggtaga caacgtcgcc
      421 gtggtgctca tggtgtcatc tccgcggtgc tgtggctttg ccggtgacgt gaccggcggc
      481 gtcgtagaac gtggtcgtgc cttccgcgtt ggtgcgcgtc ttgccggtga cgcgaccggc
      541 ggaatcataa aacgtcgtcg cgccgttgcg cgtcgtggcc ttgccggtga cgcggccggc
      601 ggcgtcgtgg aaagtcgtgg tttgtgccgc ggcggcgctc gacagcgcca gcgcgaggcc
      661 gagcgcggcc ggcggggtga aggtgaccgt ggggcgcaac agcatggtga tcggggggct
      721 catagacggc cggcggcggg gagtatagga cacgcccaat ggcgtccaag acgccatggc
      781 ttcgtattta tattcttaga tataaaaccc aaaacctaat agagcattgg ccttgaccgg
      841 cggacgcaat aggctttcag gcgtgcggtg cggcggccga gcgtcgaaac atcgataggg
      901 gagaagtagc tcagtccgaa gtggctcagt ctaatgtcag gagaagcacg atgatacgca
      961 gagtcttgtc aatcggggcg gcggtcgcgg ccctcagctt gctggcgtcg ccggttgttg
     1021 ttgctcagga gcgcggcgga acgctggtca tggtggttca accggagccg ccgacacttg
     1081 catcctatct cagcacatcg ggaccgatcg gccaagtgac gaccaaggtc ttcgacggat
     1141 tgctcgaata caatttcgat ctcaagccgc agccgagcct cgcggaatcc tgggcggtca
     1201 gcgacgacgg aaacaccatt acattcaagc tccgcaacga cgtgaagttc cacgacggcc
     1261 accccatgac cagcgccgac gtgaagttca gcatcatgga ggtcctgaag aaggtacatc
     1321 cccgcggcgc aaacacgttc cgtgagttga tcgacatcga gaccccggac gattacacag
     1381 cgattttcaa gctctccaac ccggccccct atatgctgat ggcgttatcg agctatgaat
     1441 cgcccatcat tccgcagcat gtctacggca agggcgatat caagcagcac gagaatgcca
     1501 acaagccaat cggctccggc ccgttcaagt tcgtcgaatg gcggcgcggc cagtacatgc
     1561 ggttcgatcg caatcccgac tattggaaga aaggccagcc ctatctcgac cgcatcgtgg
     1621 cgcgcttcat cgccgattcc tcgacccgga cggccgcgat cgagagcggc gaggcgcatg
     1681 tcgccggctt cggcgcgttg ccctacagcg acatcaagcg gctggacgcc ctgccgcaat
     1741 tggcggcgac aacgcgtggc tacgaaatgc agtcgccgat cgtgcagctc gatttcaaca
     1801 cccgcaagcc gccgttcgac aaggcaaagg tgcgccaggc ggtctcctat gtcatcaacc
     1861 gcccgttcgt gatcgacaat gtctggttcg gtttcggcaa gccggccacg ggtccgatca
     1921 gctcgaactt cgctgtcacg gggatgtata ccgacaaggt gctgcggtac gacgtgccag
     1981 acgccaagga gcgggcggag aaactgctcg atgaggccgg ctatccgcgc aaggcggacg
     2041 gcacgcgctt cgagatcacg cacgacatca cgccttatgg tgaggagtgg cagcgcttcg
     2101 gcgaatacac tcaacaggca ctggcggcgg tggggatcaa gacgacgctg cgcacggagg
     2161 atgtgccgac ttggctgaag cgcatctata ccgattatga ttttaatctg accaacaact
     2221 ggatccagac gctcgcggac ccggtgatcg gcgtccaccg cctctatcat tccaaccaga
     2281 tccggcctgg cacggtgttt gtcaacggct cgggctggtc gtcgccggaa accgacgcac
     2341 ttatggacaa ggcgacggtc gaggtggacg cgaagaagcg caacgcactc tatcacgagc
     2401 tacagaccaa gctcgtcgag gcggcgccct atgtctgggt gcacgagctc cagttcgtca
     2461 cggtctataa caccagtttc aaagatctga tcgtaagtcc gctgggcctg tacacgtcgt
     2521 tctcgcaggc gtatcaggcg aagaaatgat ctggtggaga cggagagctt tgcgcggtaa
     2581 atgttcactg tgggtgctct ccgtctcatt cacaccgatg ttgactggtc gtgacttctc
     2641 ccgccgtcct cctgcgatat cttgtccgcc gtttggcgca ggcgattccc attgtgcttg
     2701 ggatcgcggt gctgaatttc ctgcttctac agctggcgcc aggcgacgcc gccgatgtcc
     2761 tggcgggtga agcgggttcc gcgacgccgg aatacatggc gcaactgcgc gagcggttcg
     2821 ggctcgatca gcccgtgtac gtacagctca tgctgtatct caagaatgtt gtgacgctgg
     2881 atcttggcta ttccttccgg cacggcatgc cggttctgca attgatcctg ggccgcctct
     2941 ggccaacact cctattgatg ggaacgacgc ttgtcctgtc cgtctcggtc ggcgtgctgc
     3001 tggggcttct tgctgccacc cgagtccata gctggcggga caatttgatc tccattctcg
     3061 ccatcattgc ctatgcgacg ccgctgttct ggatcgggct aatgctgatc gtgttattct
     3121 caatcaatct tggttggttt ccagccagcg ggatggagac ggtggccgcc ttctatgagg
     3181 gctgggaccg ggccgtcgac attgcctggc atctcgtgct gccggcgctg acgctgtctt
     3241 gcttctattt ggctctctat acgcggctca tgcgggcgac ggtgctggaa cagcagggca
     3301 tggactatgt caccacggcg gaggcgaagg ggctcaccga gcgccagatc accttccgcc
     3361 acatccaacg caacgcaatt ctgcccgtgg tgaccatggt gggaatccag atcgggagcc
     3421 tgctcggcgg ctcggtcatc gtcgaatcgg tgtttggttg gcccggattg ggcctgcttg
     3481 cctatcagtc gctgttcgcc cgcgacttca atctgctttt gggcattttc tttttctcgg
     3541 cgtgcctggt cgtgatcatc aatctgctgg tcgatctgct ctatctggcg ctcgatcctc
     3601 ggatcgagat ccgctagcgg tcgggggagg gcggagcgat gcggggtttc tggggtcgat
     3661 acgcgcgaag ccggtccggc gtgctggggc tggcgatcct gaccacgatc attctcgtcg
     3721 cgctgcttgc gccggtgctg tttccgtaca atcctttcca gatcgtcgac aagccgttcc
     3781 tgcccccctt cggggactat gtgctgggca ccgacagtct ggggcgcaac atcgccgccg
     3841 ggctcgccta tggcgccagg acatcgctgc tgatcggagt gttggcgacg ctgctcgcgg
     3901 tagggtcggg cgtgatcgtc ggcggtctcg caggctatta cggcggctgg ctggacgacg
     3961 ccctcatgcg cgtcaccgaa ttcttccaga ctatcccctt cttcgttttc gcaatcgtcc
     4021 tggtcgccat cctctcgccg tcggtccaga acatcatcat cgccatcgcc atcgtgacgt
     4081 ggccgcctgt cgcgcgtctg gtgcggggcg agttcatggc catgcgaagc cgcgatttcg
     4141 tctctgcttc ggtctgcctc ggcacctccg actacgacat catcgcccgc gatatcctgc
     4201 ccaactgcct gtcgccgatc atagtcaccg gctcgctgat ggtggcgacg gccatcctca
     4261 tcgagtccgc gctcgcgttc cttgggctcg gcgacccgaa cgtcatgagc tggggcttca
     4321 tgattgcttc gggccgcacg ttcctgcgct cggcgtggtg gctgtgcgcc atgcccggca
     4381 ctgccattct actcgccgtc ctgtccatca acctggtcgg cgagggcctt aacgatgctc
     4441 tcaatcctcg gcttcgcgac gtataggcat ggcgatcctg aagctccgca acctgaccgt
     4501 cgatctgccg cccggcgccg atcgcgctca cgccgtggag gacgtgtcgc tcgacgtcaa
     4561 cagcaatgag attctctgcg tcgtcggcga atcgggctcc ggcaagtcgg tcatgggtcg
     4621 caccgtcatg ggtcttctcg ggcgcaccag actcaagccc tccgccggtc gcgtcctgtt
     4681 cggcgaggag aacctgctca ccgcttcacc ggcgcggatg cggcagatcc gcggcgaccg
     4741 catctcgatg gtcttccagg agccgatgac tgcgctgaat ccgttgatga cggtgggcag
     4801 acagatcgac gagctgctca agatccatcg cacaatgtcg catggagacc gaaagaagcg
     4861 catccacgac ctgttcgcgt cggttctgct gcccgatccc gaacgcatct atgcttccta
     4921 tccgcaccag ctatctggcg gccagcgcca acgcgccgtc attgccatgg cgctgatcct
     4981 ggagccggat gtggtcatcg ccgacgagcc gacaacagcg ctcgatgtca caacccaggc
     5041 gcagatcctg aagctgatca aggacctcca ggcggcccac cgcactgcct tcatgttcat
     5101 cactcatgac ttcggcgtcg tggccgagat cgcggaccgc gtcgccgtca tgcagcacgg
     5161 ccgcgtgctc gagacctgca cgacggccaa tgtgctcgaa caccccaaga acgactacac
     5221 gcgtacgctc cttgaggccg tgccgcggct ggttccgcgc gcgaagtgcg aactgacgaa
     5281 cccagagaat gtccttgccg tgcgcgacct gtcgaagacc tttggtgcac gcagacggtt
     5341 cttccgtcgt gccaggcgag gtatcgaggc cgtcaagagc gtgtcgcttg cgttgcggcg
     5401 cggcgagacg ttaggcatcg taggcgagtc cggatcgggc aagagcacgc ttgcgcgctg
     5461 cgtcgtgcgt ctgatcgagt cggacgccgg tgagatacgc ctgggcgaca tagacttcag
     5521 ggcgctgtcg cgggcacaac tgcggccgta tcgcaaaaag atccaaatgg tcttccagga
     5581 tccttatggg tcgcttaatc cccgcctcac cgttgggcag ctcgttgcgc aaggcccgat
     5641 cgtgcacggc gagtcgtgcg aggcggccat ggagacggtg cgcgaattgc tgcgcctggt
     5701 cgggctcgaa ccattcgtcg ccgaccgtta cccacacgag ttctccggcg gtcagcggca
     5761 gcggatcggt attgcgcgcg cgctggcgct gagaccgcag attctggtcg ccgacgagcc
     5821 ggtctccgcc ctcgatgtgt cggtgcaggc gcaggtgctg cgcctgcttg acaacattcg
     5881 cgaccgcttc aatctggcga tgctgttcat aacccacgat ctacgcgttg ccgcccaggt
     5941 gtgtgatcgc gttgcggtca tgcgctacgg ccagatcgtc gaggaggggc cgaccgagcg
     6001 gctgttcgct gcgccggaga acgactatac gcgcgagctc ctggccgcgg tgccgggcaa
     6061 gcaatgggaa gccattcgcg atgcccggcg ggcgcaggcc gccggcgtta tccagaccat
     6121 ctgaa
//
LOCUS       DATLJT010000865         3181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2813854,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000865 DATLJT010000000
VERSION     DATLJT010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813854"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="lysK"
                     /locus_tag="VM910_19865"
     CDS             complement(<1..698)
                     /gene="lysK"
                     /locus_tag="VM910_19865"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796481.1"
                     /note="class I; LysRS1; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri this enzyme charges both tRNA molecules for lysine
                     that exist in this organism (but the tRNALysUUU very
                     poorly) and in the presence of LysRS2 can charge tRNAPyl
                     with lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVI64818.1"
                     /translation="MTAIDAPTATLREVAELSHAWPFEEARKIVARLKRRPKGEVDEV
                     IFETGYGPSGLPHIGTFGEVARTSMVRHAFRVLTEDKVKTRLIAFSDDMDGLRKVPDN
                     IPNKELVAQHLGKPLTRVPDPFGTHNSFAEHNNARLRTFLDQFGFDYEFMSSTACYTC
                     GRFDDALLTVLHHFEQVMEVMLPSLREERAQTYSPFLPIDPESGIVLQVPVVEHDAEA
                     GTITYEHPDRGERVT"
     gene            complement(883..1773)
                     /locus_tag="VM910_19870"
     CDS             complement(883..1773)
                     /locus_tag="VM910_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI64819.1"
                     /translation="MDLALRAFFAATLFGAVAAVLVAGVLAAAPPVRAQDGMTLPSGA
                     GVPGFWDPRRRPERPDLSRLQSIRFLTELDYPPFNYAGPDGNPVGFNVDLARQLCDEI
                     KIACTIQARRFELLLDALDDNRGDAVIASIAITPATRRRADFTDPYYRTPARFVARID
                     SPIRDVLPELVEGKKIAVVAGTAHEAFLKQMFTAAEVRSYANAEAAREALRNKDVDLL
                     FGDGITLAFWLNGSDSAGCCVFRGGPYLESRFFGEGVGIVVKRGNDLLRQALNWALFR
                     DWEKGGFTDLWLRYFPISPF"
     gene            complement(1800..2375)
                     /locus_tag="VM910_19875"
     CDS             complement(1800..2375)
                     /locus_tag="VM910_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVI64820.1"
                     /translation="MLLFAAFLGGLFLFFGAILFVTGRAPSPVGSAIAAVGGPFHLED
                     QNGKPFSDQDMKGRPFLVFFGFTHCPDICPATLFEISQVLHTLGPDADRTGALFITVD
                     PERDTPSVIKDYLSNFDPHLHGLTGDQASINAALRAYRVYAKKVPLDNGDYTMDHTAV
                     VYLMDKDGHFVAPFNVSRTAEQEAADLRRYF"
     gene            complement(2413..2574)
                     /locus_tag="VM910_19880"
     CDS             complement(2413..2574)
                     /locus_tag="VM910_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64821.1"
                     /translation="MPQILARPRVSGSVFVASALVALALIGTGLLWAHYGTAVFFETI
                     RAGFTACFG"
     gene            complement(2677..3111)
                     /locus_tag="VM910_19885"
     CDS             complement(2677..3111)
                     /locus_tag="VM910_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64822.1"
                     /translation="MKWQAALAGALASALVTCAASADVRIVGDPGGEVSSYVQKFNEI
                     RASGQRIVIDGPCLSACTLFTGIVPRDHVCVTRRAALGFHAASYYNDASRSLVPTRQG
                     SALVMRLYPPEIRSWISHHGGLRPHMIVMRGRELSTLYPICQ"
BASE COUNT          663 a          953 c         1001 g          564 t
ORIGIN      
        1 gtaacgcgtt cgccccgatc gggatgttca taggtgatgg tgccggcctc tgcgtcatgc
       61 tcgacgaccg gcacctgcag tacgattccg ctttccggat cgatcggcag aaacggcgaa
      121 taggtctgcg cccgctcctc gcgcagcgac ggcagcatca cctccatcac ctgttcgaaa
      181 tggtgcagaa ccgtgagcag cgcatcgtcg aaacgtccgc acgtgtagca cgcggtcgag
      241 gacatgaatt cgtaatcgaa gccgaattga tcgagaaagg tgcgcagccg cgcattgttg
      301 tgttcggcga aactattgtg agtgccgaac gggtcgggca cgcgggtgag cggtttgccg
      361 agatgctgcg cgaccagttc cttgtttggg atattgtcag gaactttgcg cagcccgtcc
      421 atgtcgtcgg agaacgcgat cagccgggtt ttgaccttgt cctcggtgag gacgcgaaag
      481 gcatggcgca ccatcgaggt gcgcgccact tcgccgaatg tgccgatgtg cggcaggcct
      541 gagggcccat agccggtttc gaaaatgact tcgtccactt cacccttcgg ccgccgcttg
      601 agccgcgcca cgatcttgcg agcctcttcg aatggccagg cgtgggaaag ctcggccact
      661 tcgcggagcg tggcagtggg agcgtcaatc gcggtcatga cgtgcttggt cgatctacag
      721 cttcaattgt cgtgcggaac actagaatat tgtttgctag gtgtccccta tcgattccga
      781 agttcgtact agcggccgca aatgctaacg aacttcggaa tcgggacact cggtagcgcc
      841 catcgcatcg tcaaatgggt atcgtcaaac gcgatgatcg gctcaaaacg ggctgatcgg
      901 aaaatagcgt agccacagat ccgtgaagcc gcccttctcc caatcccgaa acagggccca
      961 gttcaaagcc tgtcgcaaca ggtcgttgcc gcgtttgacg acaatgccca cgccttcgcc
     1021 aaaaaatcgg ctttctagat acggaccgcc gcggaatacg cagcagccgg cagaatcgga
     1081 accattgagc caaaaagcca gcgtgatgcc gtcgccgaac aggagatcga cgtccttgtt
     1141 gcggagcgct tcgcgcgcgg cttccgcatt ggcgtaggac cgcacctcgg ccgcggtgaa
     1201 catctgcttg aggaaggctt catgcgctgt gccggcgacg acggcaattt tcttgccttc
     1261 gaccagttcc ggcagcacgt cgcggatcgg gctgtcgatg cgagcgacga agcgcgccgg
     1321 cgtgcgataa tagggatcgg taaaatcggc gcgccggcgc gtggccggcg tgatcgcgat
     1381 cgaagcgatc actgcgtcgc cgcgattatc gtcgagcgca tcgagcaaca gttcaaatcg
     1441 ccgcgcctgg atggtgcagg cgatcttgat ctcgtcgcag agctggcggg caaggtcaac
     1501 gttgaaaccg accggattgc cgtccggccc cgcatagttg aacggcggat aatcgagctc
     1561 ggtcagaaag cgaatcgact ggagccgtga aaggtccggc cgttccggcc gccgccgcgg
     1621 gtcccagaag cccggaacgc cggctcccga cggtagcgtc atcccgtcct gcgcccggac
     1681 cggcggtgct gcggccaaaa cccccgcaac caagacggcc gcgaccgcgc cgaagagcgt
     1741 ggcggcaaag aatgctcgta gcgccaggtc cataggccgt ttcagttggg ccaaatccat
     1801 cagaagtagc gccgcagatc ggccgcttcc tgctccgcag tccggctgac gttgaacggc
     1861 gcaacaaaat gcccgtcctt gtccatgaga tagaccaccg cggtgtgatc catggtgtag
     1921 tcgccgttat cgagcggcac tttcttggcg tagacgcgat aggcccgcag cgccgcattg
     1981 atcgaggcct gatcgccggt caggccgtgt aaatgcgggt cgaaattcga caggtaatcc
     2041 ttgatgaccg atggcgtatc gcgctcggga tcgacggtga tgaacaatgc tcccgtgcga
     2101 tcggcatcgg gtccgagcgt atgcagcacc tgcgagatct caaacagggt ggccgggcaa
     2161 atatcgggac aatgggtaaa gccgaagaac accaggaagg ggcgtccctt catatcctga
     2221 tcgctgaacg gcttgccgtt ctggtcttca aggtgaaatg gcccgccgac cgcggcaatc
     2281 gcggaaccaa ccggcgacgg cgcgcgtccg gtgacgaaaa ggatggcgcc aaaaaacaga
     2341 aacagcccgc cgagaaaagc ggcgaacagc agcatgacgc gagatgtatt cgccttcacg
     2401 agcggtttcc cgttagccaa agcaggccgt aaagccggca cggattgttt cgaagaacac
     2461 ggcggtgccg taatgggccc agagcaagcc ggtgccaatc agcgcgagag cgaccagcgc
     2521 gctggcgacg aacacagacc cagaaactcg cggtcgtgcg aggatttgtg gcatggcgga
     2581 catccggccc ggtttagata ggccggaatt agcgttccga caacggcgaa aagcaccgca
     2641 accgccgcgg tgccctgccg cttgcttgcc cgataactac tggcagatcg ggtacagcgt
     2701 gctcaattcg cggccgcgca tcacgatcat gtgcggccgc agcccgccat ggtggctgat
     2761 ccacgagcgg atttccggcg gatagagccg catcactagc gcgctgccct ggcgggtcgg
     2821 caccagcgag cgtgacgcat cgttgtaata ggaggccgcg tggaagccca aggcggcacg
     2881 ccgcgttacg caaacgtggt cgcgcgggac aatgccggtg aacaaggtgc aggccgacag
     2941 gcacggcccg tcaatgacaa tccgctggcc ggaagcgcgg atctcattga atttctgaac
     3001 gtaggaggag acctcccctc caggatcgcc gacgattcgc acgtcggcgg atgccgcgca
     3061 cgtcactagg gccgacgcga gcgcgccggc caaggctgct tgccatttca tggagtaccc
     3121 tctccccgct caatcgcgcg cagtatttaa ccggcattgt ggcaagagtt cggggattcc
     3181 c
//
LOCUS       DATLJT010000866         2849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2123721,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000866 DATLJT010000000
VERSION     DATLJT010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123721"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VM910_19890"
     CDS             complement(<1..829)
                     /locus_tag="VM910_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HVI64823.1"
                     /translation="MPANNAFHIAVLPGDGIGLEVMAPALEVLRKIEAKSDLAFRFTE
                     APAGANHYRETGKSMPDSTVRLCEEADAILLGACGLPSVRYPDNTEIMPQVELRFHFD
                     LYAGVRPARLIPGVPSPIVGADQRGIDMVVIRESTEGLFASMGKGVVTHDDARETLVI
                     TRKTSERLFEFSFRLAERRKARGRPGALTCVDKANVFKAFAFFREMFDEAARRHPEVR
                     ADRLYVDACSALLVKRPWDFDVMVMENMFGDILSDLTAGLIGGMGMAPSADIGDRHAV
                     "
     gene            complement(938..2008)
                     /locus_tag="VM910_19895"
     CDS             complement(938..2008)
                     /locus_tag="VM910_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HVI64824.1"
                     /translation="MKSFQVVDFNAPLKEVEQPTPRPQGTQVLIRVKAAGVCHSDLHI
                     WEGGYELGHGRKPLSLKDRGVSLPRTMGHETVGEVVAFGPDVKESDKGGLKIGDVALA
                     YPWLGCGKCPTCLAGDENMCAVKPNALGVFCDGGYADHMMMPHPKYLLDLKGLDPVTA
                     APYACSGVTTYSALKKVEKDFGTPIVIFGAGGLGLMALSLLKAMGGKGAIVVDIDARK
                     REAAEKAGALATVDGKAGDALEQLMKKAGEPIRAAIDLVGNAQTAQLGFDCLTKGGKL
                     VIVGLFGGGAPWALPLIPIKAVTIQGSYVGNLRETKELLDLVRAKKVAPIPVTPMPLA
                     KANDALVDLQKGKLVGRAILVP"
     gene            complement(2124..>2849)
                     /locus_tag="VM910_19900"
     CDS             complement(2124..>2849)
                     /locus_tag="VM910_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI64825.1"
                     /translation="LAHLAYQPGRERPDYCGIYHADVLTGTYAFGAISAALYQRHQTQ
                     RGQHIDVSMLESMLTLTLNEVQWSQFEVAPPSRPMFGPIETTDGYVMVAIASEKTFQG
                     LMAVIGHPEWVSDPRFAIYADRRNNWAELMDGVEAWSRTVATEQCLAALSGEGVPSSA
                     YRTVAEALADPQIAHRQALSEVEDLGGTFKVLNLPFRMSDARVAAGKRMASLGEHTRA
                     LCQEAGLSETPSSPQRRLVAESR"
BASE COUNT          517 a          937 c          873 g          522 t
ORIGIN      
        1 acaccgcatg gcggtcgccg atatcggccg acggcgccat ccccatgccg ccgatcaggc
       61 cggcagtgag gtcggagaga atgtcgccga acatgttctc catcaccatg acgtcgaaat
      121 cccagggacg cttgaccagc aaggccgagc aggcatcgac atagaggcgg tccgccctca
      181 cttccgggtg acgtcgagcg gcctcatcga acatctcgcg aaagaacgcg aaggccttga
      241 acacattggc cttatcgacg caggtcaggg cgcccggccg gccgcgcgct ttgcggcgct
      301 cagcgagcct gaacgagaat tcgaacaggc gttccgacgt cttgcgcgtg atgaccagcg
      361 tctcgcgcgc atcgtcatgg gtgacgacgc ccttccccat cgaggcgaac aggccctcgg
      421 tagattcccg gatcacaacc atgtcgatgc cgcgttgatc cgcgccgacg atcgggctcg
      481 gcacacccgg aatgagacgc gccggacgca cccctgcgta gaggtcgaag tgaaagcgca
      541 attccacctg cggcatgatc tcggtgttgt cggggtagcg caccgaaggc agcccgcagg
      601 cgccgagcag gatcgcgtcc gcctcttcac acagcctgac ggtcgagtcc ggcatcgact
      661 tgccggtttc acggtaatgg ttggcgcccg ccggcgcctc ggtgaagcgg aacgcgagat
      721 ccgattttgc ctcgatcttg cgcaggacct cgagcgccgg cgccatcacc tccaggccga
      781 tgccgtcgcc agggaggact gcgatgtgga atgcgttgtt tgcgggcatg gaatttcctc
      841 ttgctccggg gtcttgcacc gtcgttgcga gcgaagcaat ccagagcctg gaaagaaccg
      901 cccgttgctg gattgcttcg caatgaggag gaaaggccta cggcaccagt atcgcgcggc
      961 cgaccagctt tcctttctgc aagtcgacca gcgcgtcgtt ggctttggca agcggcatcg
     1021 gcgtcaccgg gatcggcgca accttcttgg cgcgaacgag gtcgagcagt tcctttgtct
     1081 cccgcaaatt gccgacatag ctgccctgga tggtgacggc cttgatcggg atcagcggca
     1141 acgcccacgg cgccccgcca ccgaacagac cgacgatcac gagcttgccg cccttggtca
     1201 ggcagtcgaa gccgagctgc gcggtctggg cattgccgac aagatcgatc gcggcgcgga
     1261 tcggctcgcc tgcctttttc ataagctgct ccagcgcatc gcccgccttg ccatcgacgg
     1321 tcgcgagcgc gcctgccttc tccgctgctt cgcgcttcct ggcatcgatg tcgacgacga
     1381 tcgcgccctt gccgcccatc gccttcagaa gcgacagtgc catcaggccg aggccgccgg
     1441 cgccaaagat cacgataggc gtgccaaaat ccttctcgac cttcttcagc gccgaatagg
     1501 tggtgacgcc ggagcaggcg tagggcgcag cggtaacggg atcgagccct ttgaggtcga
     1561 gaagatattt gggatgcggc atcatcatgt ggtcggcata accgccgtcg cagaatacac
     1621 cgagggcatt cggcttcacc gcgcacatgt tttcgtcgcc ggcaagacag gtcggacatt
     1681 tgccgcaacc gagccacgga taggccagcg ccacgtcgcc gattttgagg ccgcccttgt
     1741 cgctctcttt tacgtcaggg ccgaaagcca cgacctcgcc gacggtctca tgccccatag
     1801 tgcgcggcag cgacacgccg cggtctttta gcgacagcgg tttgcggccg tggccgagct
     1861 cgtaaccgcc ttcccagata tgcaaatcgc tgtgacagac ccccgcggcc ttcaccctga
     1921 tcaggacctg cgttccctgc ggccggggtg tcggctgttc gacctctttc aagggggcat
     1981 tgaaatcgac aacctgaaag ctcttcatgg catacctccc gatgtttttg ttcttgatcg
     2041 gattttcgcg cctgcggcgc tgtcctgccg cagaatcgaa cgataccgtc tctttatgag
     2101 cggcgcgccg caagggcaag ccgtcagcgc gactctgcga cgagccgcct ctgcggcgat
     2161 gacggcgtct ccgaaagccc ggcctcctgg cacagcgcgc gcgtgtgctc gcctaaactc
     2221 gccatccgct tccccgccgc caccctggcg tccgacatcc ggaacggcag gttgagaacc
     2281 ttgaaagtgc cgcccaagtc ctcgacctcg gacagggcct ggcgatgggc gatctgcgga
     2341 tcggcgagcg cctctgcgac cgtgcgatag gccgaggacg ggacgccttc gccgctcaac
     2401 gcagcaagac attgttccgt cgcgaccgtt cgcgaccagg cttccactcc gtccatcagc
     2461 tccgcccagt tgttgcggcg atcggcgtaa atcgcaaagc gcggatcgct cacccattcg
     2521 gggtgaccga tcaccgccat caggccctgg aaggtctttt cgcttgcgat cgcgaccatg
     2581 acgtagccgt cggtggtctc gatcggaccg aacatcggac gcgagggcgg cgccacctcg
     2641 aactgcgacc actgcacttc gttcaaggtc aaggtcagca tcgattccag catcgatacg
     2701 tcgatgtgct ggccgcgttg cgtctgatgg cgctgataga gcgccgccga aatcgcgccg
     2761 aatgcatacg tgccggtgag gacgtcggcg tgatagatgc cgcaataatc gggacgttcg
     2821 cggcccggct gataggcgag atgcgccaa
//
LOCUS       DATLJT010000867         7252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4192312,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000867 DATLJT010000000
VERSION     DATLJT010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4192312"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VM910_19905"
     CDS             complement(<1..256)
                     /locus_tag="VM910_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HVI64826.1"
                     /translation="MAISDLPVLSALRTKMQWHQARQRVLSENVANSDTPNFRPRDLV
                     EPKFDASGARAGSTGTLVMAVTSTGHIAPSGGAAQSFDVNS"
     gene            634..1575
                     /locus_tag="VM910_19910"
     CDS             634..1575
                     /locus_tag="VM910_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVI64827.1"
                     /translation="MQVTFIFAFVAVLALMLLAAWLIRRFANSRLGTNTNRGRMPRLA
                     VIDAAAVDGRRRLVLVRRDNIEHLLMIGGPTDIVVEANIVRAQARSEQFPQRPPAAEP
                     PPRIAPLPDAAVWADGDSARIDAFDQHPETQMPEPPPRPSRPPFADEVRRQAPVPPER
                     RGERTTDPLTGFAPEPLAPRPEPRPEPITPRLPRTEPMIARASRQAEPKAPPVVRAAP
                     PPPPPPPPPPAPAQNADQNLAEMAQRLEAALRRPAGSADAPPVAPEPPAPPPSRATRS
                     EPAVPPPPPPAPTPKSSFENLEEEMASLLGRPKSSSS"
     gene            1572..2324
                     /gene="fliP"
                     /locus_tag="VM910_19915"
     CDS             1572..2324
                     /gene="fliP"
                     /locus_tag="VM910_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509903.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HVI64828.1"
                     /translation="MKRARAPRRVLFIATLIAAGALAAPAFAQDISINLGGSGGGVTE
                     RAIQLIVLLTVLSIAPSILIMMTSFTRIVVVLSLLRTAMGTATAPPNSVIIALAMFLT
                     AFVMGPVLQKSYDDGIKPLIANQITVEDALQRASVPLRSFMQKNVREKDLKLFLDLSG
                     EPPPATPEELSLRILVPAFMISELKRAFEIGFLLFLPFLIIDLVVASILMSMGMMMLP
                     PVVVSLPFKLIFFVLVDGWSLVAGSLVQSYGG"
     gene            2457..3347
                     /locus_tag="VM910_19920"
     CDS             2457..3347
                     /locus_tag="VM910_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64829.1"
                     /translation="MQQHFDRAAEDLGNSIHLEHVNVQVPDQRLATLFYVTGLGLTRD
                     PYLMVSDTNMWVNVGRSQFHLPTGAAQVLRGRTGLVISGRQALLDRLASVAPKLAGTA
                     FGFSEHNDYVEATCPWGNRLRCHEPDAERFGRITLGIPYVEFDVPVGTAKAICAFYPE
                     IMEIPAKFRNGDGTLARVKVGKDQYLQFRESTAPQAEFDGHHVQIYITNFSGPYRRLK
                     ERGLVSQEDNQYQYRFRDIVDPATGKHLFTVEHEVRSATHPMYLRPLVNRDPAQTNRT
                     YAHGHDQWPWAMGPDEYDRR"
     gene            complement(3417..3731)
                     /locus_tag="VM910_19925"
     CDS             complement(3417..3731)
                     /locus_tag="VM910_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HVI64830.1"
                     /translation="MIKVSVMYPNKPGARFDHDYYRDKHMPLVKARMGAACKSYTVDK
                     GLAGGAPGQPATYVGMCHIFCDSIESFQTGFGPHAKEIMADIPNYTDLAPVIQISEVV
                     VG"
     gene            3952..4578
                     /locus_tag="VM910_19930"
     CDS             3952..4578
                     /locus_tag="VM910_19930"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HVI64831.1"
                     /translation="MLSADDNENKPIGKPGQRKKAEQTGKKTEPRRRKERQPQEATPE
                     QKQIAAEQVNASVSAPISEEASASVGEQVIAPISEQISAPASEQSSADVAVAATESSA
                     SDTSLPAPSPLASVPPSELAPVNLQTIAEAYRDYSRKSFEQTSCFFERLAGARSLTSA
                     FEIQAEFARQSYETFVAQSRRIRELHGELARQRLERFERLMTKTTRAN"
     gene            complement(4726..4962)
                     /locus_tag="VM910_19935"
     CDS             complement(4726..4962)
                     /locus_tag="VM910_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64832.1"
                     /translation="MSKPVKTAAELIAMAREELKVHADCPDGLSISVIPSGDSWEFRA
                     TADEATQAKPGYPECVAMLVQIGDHLAKQYDAKG"
     gene            complement(5108..>7252)
                     /locus_tag="VM910_19940"
     CDS             complement(5108..>7252)
                     /locus_tag="VM910_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI64833.1"
                     /translation="LIFADKVEAPPQPLPVMRNITDPALANVVVPLANPGRLHRLVDP
                     DAGDTLLVVTATPPIRGVIKRQDFVDFALLDSAHGIAIRPNSEDVGVEVAPDKVIVGK
                     PGGLTLSSVKVSAERAPAAVRPIFDVEEWRKNQEESFTRREDELVLAAATAEADRRPQ
                     LRLALARFYIARGMYWEAKGVTEFLLADSNLKNEESVAIMVHAIACLLVDRPDLAAKD
                     LANPLIGSNYDAQLWRALTYARQEKWAEAREKFKNVEFAIASLPLDLQRIVTADAMRA
                     SLEVKDYAGAAKRRAELDVIGVPAEQKATIAVLRGRLAEALGNEKDALDEYAFAGASS
                     DRQAAAEAKQLEIALKQKRDEIRQADALRELETLSAVWRGDAVEVKTLQMLSLIYNVT
                     ARYPESLAAARTATRLRPNSEASRAAQDNAQALFSEIFLTRKGDDLPPIDALGMFYEF
                     RELTPIGRRGDEMIRRLADRLVAVDLLDQAAELLQYQIDHRLEGSARAQVAAKLAMVY
                     LTSHKPDLAIGALRSSRIADLSGELRQQRLLLEARAQSDIGRHDLALDIVSNITGREA
                     IRLRSDIYWAARRWRESAEQIELYYGDRWRDFKPLNGIEKSDIIRAVVGYALAEDAIG
                     LARFREKYAALMSGESDKVAFDIASKPSAGNSGEFAEIARIAAGVDTLDGFLREMKTR
                     FPDGTTRATVPDPEPTGALPRIEGVKQVSAAR"
BASE COUNT         1264 a         2397 c         2253 g         1338 t
ORIGIN      
        1 tgctgttgac atcgaaactt tgggcagccc cgccggatgg cgcgatatgg ccggtcgagg
       61 tgaccgccat gaccagtgtc cccgtcgagc ccgcccgggc gccggacgcg tcgaatttcg
      121 gctcgaccag gtcgcgcggc ctgaagttcg gcgtgtcgga gttggcgacg ttctcggaca
      181 gcacgcgctg gcgcgcctga tgccattgca tcttggtgcg cagcgccgac aagaccggaa
      241 gatcgctgat ggccatagcg cgccgcttcc caggttcagt cgccgggcac ctcgcggcaa
      301 ctaggcagat tttgccgcgt gcatggttaa cagggggtta agacgtgcga attcggcccg
      361 cgggttgtcc cggcccgaac gaccgccgcc ggtcaattcg agcgcaattt cgccacgatg
      421 gctgcgttaa ccccgtcgct tcatgaacgc cgaaatccca agaagtcgtt ttggttcagc
      481 cgattcatga gttattaaga tatttggggc ggcggggtcc tggccgtatg ggggttgagg
      541 gtttagttcc gcgctcaggc ttcgtttgcc ttttgaagtg ccgcgtcagg ggacgcgagg
      601 cttgacgaaa gtgccatttg ccggggactg gacatgcagg tgacctttat ttttgcattt
      661 gtggccgtac tggccctgat gcttcttgcc gcctggctga ttcgccgatt cgcgaacagc
      721 cgcctcggca ccaacaccaa tcgcgggcgg atgccgcgcc ttgcggtcat cgatgccgca
      781 gccgtcgacg gaagacggcg tctggtgctg gtgcggcggg ataacatcga gcatctcctg
      841 atgatcggcg ggcctaccga catcgtcgtg gaagcgaata tcgtccgcgc ccaggcacgg
      901 tcggaacagt tcccgcagcg tccgcctgcc gccgagccgc cgccgcgaat cgcgccgctt
      961 ccggacgccg ccgtctgggc cgatggcgac agcgcgcgta tcgatgcctt cgaccaacat
     1021 cccgaaacgc aaatgcccga gccgccgccg cggccttccc gccccccctt tgccgacgaa
     1081 gtgcgccggc aggcgccggt gccaccggag cggcgcggcg agcgcaccac cgatcctttg
     1141 accggtttcg cgccggagcc gctcgctccg cggcctgaac ctcgtcccga accgataacg
     1201 ccccgcttgc cgcgcaccga accgatgatt gcgcgcgcct cccgccaggc cgagccgaag
     1261 gcaccgccgg tggttcgcgc cgctccgccg cctccaccac cgccaccgcc gccacctgct
     1321 ccggcccaga acgcggacca gaatctcgcc gagatggcgc agcggttgga ggccgcgctg
     1381 cgtcgtcctg ctggctccgc cgacgcgccg ccggttgcgc ccgagccgcc ggccccgccg
     1441 ccatcacgtg ccacgcgcag cgagccggcc gtaccgccgc cgccaccgcc tgcgcccacg
     1501 ccgaaaagca gttttgaaaa tctcgaggaa gagatggcct cgctgctcgg acgtccgaag
     1561 tcgtcgtctt cgtgaagcgc gcgcgagccc cgcgtagagt tttattcatt gccacactga
     1621 ttgccgcagg cgcgcttgct gcgcccgcgt tcgcgcagga tatcagcatc aacctgggcg
     1681 gcagcggcgg cggcgtcacc gagcgcgcaa tccagttgat tgtgctgttg acggtgcttt
     1741 cgatcgcccc gtcgatcctg atcatgatga cgtcgttcac ccgcatcgtc gtggtgctgt
     1801 cgttgctgcg caccgctatg ggcacggcaa ccgcgccgcc gaactcggtg atcattgcgc
     1861 ttgcgatgtt tttgaccgcc ttcgtgatgg ggccggtcct gcagaaatct tacgacgacg
     1921 gtatcaagcc gctgatcgcc aaccagatca cggtggaaga cgcgttgcag cgcgcttcgg
     1981 tccctttgcg cagcttcatg cagaagaacg tccgcgagaa ggacttaaaa ctgttcctgg
     2041 atctctccgg cgagccgccg ccggcgacac cggaggagtt gtcgctgagg atcctggttc
     2101 cggccttcat gatctccgaa ctgaagcgcg ccttcgagat cggctttctg ttgttcctgc
     2161 ccttcctgat catcgacctc gtggtcgcct cgatcctgat gtcgatgggc atgatgatgc
     2221 tgccgccagt cgtggtatcg ctccccttca agctgatctt tttcgtgctg gtcgacggct
     2281 ggtcgctggt cgcgggcagc ctggtgcaga gctatggggg gtagcgaatc ctccggagcc
     2341 caagggccgc cttgagccca accgcgtggc gtttgcgcgg agattatgcg ccagcatcag
     2401 ttacccgttg ggccgcgctt gggtatgatg gcgccgacac agcccgggaa cccgccatgc
     2461 agcagcattt cgaccgcgcc gccgaagacc tcggcaactc gatccatctc gaacatgtca
     2521 atgtccaggt ccccgaccag cggttggcga ccttgtttta tgtcaccggc ctcggcctga
     2581 cccgggaccc ctatctgatg gtgtccgaca ccaacatgtg ggtgaatgtc ggccgcagcc
     2641 agtttcacct gccgaccggc gcggcgcagg tccttcgcgg ccggactggc cttgtgattt
     2701 cgggccgcca ggctctgctc gatcgcctgg cctcggtggc gcccaagctt gccggcaccg
     2761 ccttcggctt tagcgagcac aacgactatg tcgaggcgac ctgtccctgg ggcaatcgct
     2821 tgcgctgcca tgagcctgat gccgaacggt tcggccgcat tacgctcggc atcccctatg
     2881 tcgaattcga cgtccccgta ggcacggcca aggccatttg cgccttttat cccgagatca
     2941 tggagattcc tgccaagttc aggaatggcg acggcacgct cgcgcgcgtg aaggtgggca
     3001 aggaccagta tctgcaattt cgcgaaagca cggctccgca agcggagttc gacggtcacc
     3061 acgtgcaaat ctacatcacg aacttctcgg gcccctatcg ccgcctcaag gagcgcggcc
     3121 tggtctcaca ggaagacaat caataccagt accggttccg tgacatagtc gatcccgcca
     3181 ccggcaagca cctcttcacc gtcgagcacg aggtgcgcag cgccacccat ccaatgtacc
     3241 tgcggccgct ggtcaaccgc gatcccgcac agaccaatcg cacctacgcc cacggccacg
     3301 accaatggcc ctgggcgatg ggacccgacg agtacgatcg acggtagggt cgatgggggg
     3361 cacgtcgaga gacgcgcctg tgccgacctt attttttgac gcagccgccc cgctcgtcag
     3421 ccgactacga cttcgctgat ctggatcacg ggggcgaggt cggtgtagtt cgggatgtca
     3481 gccattatct ccttggcgtg cggcccgaag ccggtctgga acgactcgat ggagtcgcag
     3541 aaaatgtggc acattccgac gtaggtggcc ggctgaccag gcgcgccgcc cgcaaggccc
     3601 ttgtcgacgg tgtaagactt gcacgcagcg cccatgcggg ccttgaccag cggcatatgc
     3661 ttgtcccgat agtaatcgtg gtcgaagcgc gcgccgggct tgtttggata catgacgctg
     3721 actttgatca tgggcgtgtt ccctggctct gacttgttat gataccggtg ttatgatgct
     3781 ctccgggccg gctggaactc tgcgtcagtt tcacagatcc tgcaaccgtt cacccttcca
     3841 aatctcaaca catcaccgcc gcacgagcaa caaggcgaaa ccgcgattaa agcgcaaaca
     3901 tggcagacgt tcgcattctg attcgaactg gaaccacgga gctatctgcg aatgttgagt
     3961 gcagacgaca acgagaacaa gccgatcggg aaacccggtc agcgcaagaa agcggagcag
     4021 acaggcaaga agaccgaacc gcggcgccgg aaagaacgtc agccacaaga ggcgacgccg
     4081 gagcaaaagc agatcgcggc ggaacaggtc aacgcatcgg tcagcgcccc catcagtgaa
     4141 gaagccagcg ccagcgtcgg tgaacaggtc atcgcaccca tcagtgaaca gatcagcgcg
     4201 ccggccagtg agcagagcag cgcagacgtc gccgtcgcag cgaccgaaag ttcggcaagc
     4261 gatacctctt tgcccgcgcc ctctccgctc gcatcggtcc cgccgtccga actcgcgccg
     4321 gtcaaccttc agaccattgc ggaggcctat cgcgattact cgagaaagtc gttcgagcag
     4381 accagttgct tttttgaaag gctcgcgggc gcccgctcgc tcaccagcgc atttgaaatt
     4441 caggccgaat tcgcgcgaca gagctacgag acctttgttg cgcaatcgcg caggattcgt
     4501 gagctgcatg gcgagctggc gagacagcga ctcgagcgct tcgaacgtct gatgacgaag
     4561 accaccaggg cgaactgacc gcttccccgt cgtcagcggc gcaatccacc gatcagccat
     4621 gggacggatc cggcgcggcg ggacctgcca cgcccacctc gctcctggcc gcgcttgaac
     4681 aaggctgatc aggtcaccgc cgaggggagc agtcagaccg cgtccctacc ccttcgcatc
     4741 gtattgcttg gcaagatggt cgccgatctg caccagcatc gccacgcatt cgggatagcc
     4801 gggcttggcc tgcgtggcct cgtccgccgt cgcgcggaac tcccatgaat cgcccgacgg
     4861 aatcaccgag atcgaaaggc cgtcggggca gtcggcgtga accttcaact cctcacgcgc
     4921 catcgcgatc agttcggctg cggtcttgac tggcttgctc atatgggcag tgccctcccg
     4981 gcggtttatt tgacgagcaa attgccggtt tgccgcgccc ctgtcgaggc tcttccgcct
     5041 ctccgagcaa gcaggggcgt tgtaatggag ccttcgagcc catagagcgc ttcgatcggg
     5101 aacagcgcta cctcgccgcg ctgacctgct tcacaccctc gattcgcggc agcgcgccgg
     5161 tgggctccgg gtcaggcacg gtcgcgcgcg tagtgccgtc ggggaatcgc gtcttcattt
     5221 cgcgcaggaa gccgtcgagc gtatcgacac ccgcggcgat cctggcaatc tcggcgaact
     5281 cgccactatt gccggcgctc ggcttgctcg cgatgtcgaa ggcgaccttg tcactctccc
     5341 cgctcatcag cgccgcatat ttctcgcgga agcgcgcgag cccgatcgca tcctcggcca
     5401 gcgcatagcc caccaccgcg cggatgatgt cgctcttctc gatgccattc agcggcttga
     5461 agtcgcgcca gcgatctccg taatagagct cgatctgttc ggcggattcg cgccagcgcc
     5521 gggctgccca gtagatgtcc gagcgcagcc tgatcgcctc gcgacccgtg atattggaaa
     5581 cgatgtcgag cgcgaggtcg tggcgcccga tgtcgctctg ggcgcgcgcc tcgagcaaga
     5641 gccgctgctg gcgcagctcg ccggaaagat cggcaatgcg actggagcga agggcgccga
     5701 tcgcaagatc gggcttgtgg ctggtgagat agaccatcgc cagctttgcc gcgacctgtg
     5761 cccgcgccga gccctcgagg cggtgatcga tctggtattg caatagttcc gccgcctggt
     5821 ccaacagatc gaccgcgacc agccggtcgg cgagccggcg gatcatctcg tcgccacgcc
     5881 ggccgatcgg tgtcaactcg cggaattcgt aaaacatgcc gagcgcgtcg atcggcggca
     5941 ggtcatcgcc cttgcgggtc aggaagattt ccgagaacag cgcctgcgca ttgtcctggg
     6001 cggcgcgcga ggcctcggaa ttgggcctga gcctggtcgc cgtccgcgcc gcagcgagcg
     6061 actccggata gcgcgcggtg acgttgtaga tcagcgacag catctgcagc gtcttcactt
     6121 cgaccgcatc gccgcgccac accgccgaaa gcgtctcgag ctcgcgcagc gcgtccgcct
     6181 gcctgatctc gtcgcgcttc tgcttcagcg cgatctcgag ctgtttggct tcggccgccg
     6241 cctggcggtc ggatgaagcg cctgcaaagg catattcgtc gagcgcgtcc ttttcattgc
     6301 ctagagcctc ggcaagccgg ccgcgcagga ccgcgatcgt cgccttctgt tccgcgggta
     6361 cgccgatcac atcgagctcg gcgcggcgct tggcggcgcc ggcataatcc ttgacctcga
     6421 gcgaggcgcg catcgcgtcc gcagtgacga tgcgctgcag atcgagcggg agcgaggcga
     6481 tggcgaactc gacgttcttg aacttctccc gcgcttctgc ccatttctcc tggcgcgcat
     6541 aggtgagcgc cctccacaac tgcgcatcgt agttggagcc gatcaaagga ttggcgagat
     6601 ccttggccgc cagatccggt cggtcgacca gcagacaagc aatcgcatgc accatgatcg
     6661 caaccgattc ctcgttcttc aggttggaat cggcgagtag gaattcggtg acccccttgg
     6721 cttcccagta catgccgcgc gcaatgtaga atctggcgag cgccaggcga agttgcgggc
     6781 gccggtcggc ttccgcggtc gcagccgcca gcaccaactc gtcctcgcgc ctggtgaagc
     6841 tttcttcctg gttcttgcgc cattcctcga catcgaagat cgggcggacc gccgccggcg
     6901 cgcgctcggc cgagactttg acggaggaca gcgtcaaccc gccgggcttg ccgacaatga
     6961 ccttgtcggg cgcgacctcg acgccgacat cttcggagtt tggccggatc gcgatcccat
     7021 gggcggaatc gagcagcgcg aagtcgacaa aatcctgccg cttgatgacg ccgcggatcg
     7081 gcggggttgc ggtcaccacc aggagcgtgt cgccggcgtc gggatcgaca agccgatgca
     7141 gacgcccggg attggccagc ggcaccacga cattggcgag cgcggggtcg gtgatgttgc
     7201 gcatcaccgg aagcggttgc ggcggcgctt ccaccttgtc ggcgaagatc ag
//
LOCUS       DATLJT010000868         7768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1665518,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000868 DATLJT010000000
VERSION     DATLJT010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665518"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..2412)
                     /locus_tag="VM910_19945"
     CDS             complement(376..2412)
                     /locus_tag="VM910_19945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVI64834.1"
                     /translation="MTQLSYRADIDGLRAVAVMSVLLYHFGIVSALSGGFTGVDIFFV
                     ISGFLITSLIVADLKAGGFSLLAFYQRRIRRIFPAMLVVLAASLVAGYFFLLPGDYTS
                     LGDSAAYSAASLANWYFLQNTGYFDRAADLLPLLHLWSLSVEEQFYLVWPLLLAAFFF
                     IARKQAVVVAALVGAVVIGGLWLAVQRTGTDPKSAFFLAHLRAWELALGALLVFLPPL
                     RKSASRWKNELPTIIGVALIAISVLTLSREHSFPGLNAMPACVGAALIIWPRASETLV
                     AQMLSVPAMRFIGLISYSLYLWHWPVLVFYRHYASGAAPTFLVATGLAAACIGLAYLS
                     WRFIEQPARRRTARPFMTVGVGLAAVSTAAVAGLSVAALHGFPGRRSPDVQDMSSLEE
                     MWKWECPQHLKLEGLPPRSYCVFGASWSDARTKAVLWGDSHADHWAPIIQPVAERAGL
                     AVLLYRECPPIIEAGVVNVTLPDQPTLTRDCTESRKLAIATLRSRPDINLVILAAAWS
                     TLVPMVYNSDPTLRSPSRGLALLEQGLNDLIDQLHADGRQFVIIGDVAQWSEDPIPCA
                     LAKYSSVLMRPCRQPERSLYAYFEERIRPLNDAFRAIADKHRDVSIILPAARMCRDDG
                     CMTTLGNQFLYRDVGHIRRNLPMPTREAFAGMLGIGDITKPHARRDFGSIPDTQ"
     gene            complement(2653..3456)
                     /locus_tag="VM910_19950"
     CDS             complement(2653..3456)
                     /locus_tag="VM910_19950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI64835.1"
                     /translation="MIEDPSAQRVVDLNHAILKSRGGIVSAQSMILDFGCGSGRHTYE
                     YLEAGFPNAFGYDVKNYVNLRSAADIAHFRFDPISADGIDYPPMSKIPWPDNVFDFVF
                     ATSVFEHVREQKLAFSEIHRVLKLGGWFLNVFPSRWRPIEAHTDIPFGGVFNSPGYLK
                     LCAALGIRGNLYEENFTVDQLVEANRYFLTHGVNYLSGRQITDLLQQTFGEFEYVEDA
                     FVDHSPGRSRHLAIPLKLLPVLRQLFRFAHTRVVLARKRTSHEPMLRAG"
     gene            3734..4720
                     /locus_tag="VM910_19955"
     CDS             3734..4720
                     /locus_tag="VM910_19955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64836.1"
                     /translation="MSRTGIAMIKTGLYRVDQLSSSAKIRGFFGGTRRVSITLYEKLH
                     GMKKPEEWAEKILFSHCDARGIYKRTYACRFDQFDDMAIDCLGREFPAGRALTMHDIG
                     VSDGRTACDFFQKLAARFPHLNYYASDYEPSLMMLRSGKGGSVVTLNKKGEAIEIVMP
                     PFVFNLIKPENFLLYPINYAFFLFARAIVLRRTLAKYRAGKIEPSPLVLFCPAARELA
                     ASDGRFRLLEYDLLQPHALPCRLDAVRVMNVLNSSYFDPQQLAIAVRHIFESLAPGGL
                     LIIGSNDAAGTHVRGGIYRKLNQGFQMLAKSDADHDAHHTIMGCTAIERDGL"
     gene            4921..5325
                     /locus_tag="VM910_19960"
     CDS             4921..5325
                     /locus_tag="VM910_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI64837.1"
                     /translation="MPLPVTFDHCVVHVSDWERSNAFYRDVLGAELVRRPQGWAYRFG
                     AAQLNLHGPGFHPAEVARLPVEPGNSDLCFQWYGPIADAVTHLERCRVPIHAGPIERF
                     GAKGTGISVYFRDPDGSLLEFISYHRKDGERT"
     gene            5447..5878
                     /locus_tag="VM910_19965"
     CDS             5447..5878
                     /locus_tag="VM910_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HVI64838.1"
                     /translation="MSSRDFPPQDILVPISVGELMDKITILEIKSERLKNPSQLDNVT
                     QELAALRAVRLGDVDRVTLDKLGAELRRVNAELWDVEDAIRECDARGDFGQSFIDLAR
                     AVYRLNDERSRLKKAINLASGSRLIEEKSYSFYHPKGGDQP"
     gene            5875..6441
                     /locus_tag="VM910_19970"
     CDS             5875..6441
                     /locus_tag="VM910_19970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI64839.1"
                     /translation="MTTLHDPTVLPADIPAPQDDGAARHLTGTRLPSLPLAATDGSQV
                     DLSKLAGRTVVYIYPRTGVPGQPLPDGWNQIPGARGCTPQSCGFRDHFAELKQLGVAR
                     LFGLSTQDTAYQREAVERLHLPFPVLSDQNLELARAVKLPTFTTSGMTLLKRMALIID
                     DGVITKVFYPVFPPDKNAEEVIAWLRRA"
     gene            complement(6504..7175)
                     /locus_tag="VM910_19975"
     CDS             complement(6504..7175)
                     /locus_tag="VM910_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVI64840.1"
                     /translation="MTTISKTEHRHADASGKPGRDCPLCPRLKAFREEWRRREAQWFN
                     APVASFGPRSARLLIVGLAPGLRGANRTGRPFTGDFAGGLLYDTLKRFGFARGSYLAS
                     ADDGLTLVDARITNAVRCVPPENKPTPAEVKTCRSFLQATIAEMRNLRAIVTLGRISH
                     DSTLTALNQRRAAAPFGHGASHEIGRLRIFASYHCSRYNTSTGVLTPKMFEDVFAAAR
                     AFLGD"
     gene            complement(7153..7494)
                     /locus_tag="VM910_19980"
                     /pseudo
     CDS             complement(7153..7494)
                     /locus_tag="VM910_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7484..7765)
                     /locus_tag="VM910_19985"
                     /pseudo
     CDS             complement(7484..7765)
                     /locus_tag="VM910_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT         1542 a         2366 c         2443 g         1417 t
ORIGIN      
        1 ggccaagaag aaaaagaggg tcgcatcgcg ctaatcaagc gctctgacgc gtcgtcacct
       61 taaatgggtg cgtgagcgaa gctaaccgca tcggggcgac ggcggcgcga agctcggtga
      121 gggcccgttc ccttttcgaa ctgtgagcgg ggctacggcc tgcggcatgc gacccatgca
      181 gcaggccgta atcttatcgc ctcgacttgc gcctcgcctg cgcgccgtaa tcgcagccgc
      241 cgatgaagag cgtcgttacc gctcacattt tcggccactg cgctcaaacg cgttccgccg
      301 cgatgtgctg agccatagac gtgttcttta caaagctctg gcggatcgcc cggcgcacgc
      361 ggtcgttcgt gctcctcact gggtgtcggg tatcgatcca aaatctcggc gtgcatgcgg
      421 cttcgtgata tcaccgatcc caagcatgcc cgcaaaagct tcgcgcgtag gcatgggcaa
      481 gtttcgccgg atgtgaccca cgtcgcggta aagaaactga tttccaaggg tggtcatgca
      541 accatcgtct cgacacatcc tggctgcggg caggataatc gagacatccc tatgcttgtc
      601 ggcaatcgct cggaacgcat cgttgagcgg ccggatgcgc tcttcaaagt aagcgtaaag
      661 ggacctctcc ggttggcgac acggccgcat cagtacgctt gaatattttg ccaaggcaca
      721 ggggatcgga tcttcggacc attgggccac gtcgccaatg atgacgaact gacggccgtc
      781 ggcatgcagc tgatcgatga gatcgttgag gccttgttcc aatagcgcaa gaccgcggct
      841 cggagaccgc agcgtggggt cgctgttgta aaccatgggc acaagggtgc tccaggcggc
      901 tgcgagaatc acaaggttga tgtcaggcct ggacctaagc gtggcgatcg ccagtttgcg
      961 gctttccgta cagtcgcggg taagggttgg ttgatcgggc agcgtcacat ttacgacgcc
     1021 cgcctcgatg attggggggc attcccgata gagcaggacc gcgaggccgg cgcgctcggc
     1081 cacgggctgg atgatcggag cccaatggtc ggcatggctg tctccccaaa gcacggcctt
     1141 ggtcctcgcg tcggaccaac ttgctccgaa cacgcagtag gatcgcggcg gaaggccctc
     1201 aagcttcagg tgctgcggac actcccattt ccacatttcc tccaggctgc tcatgtcttg
     1261 gacgtcgggc gaccgtctgc ccggaaatcc gtgcagcgca gcgacgctca gcccggcaac
     1321 cgcggcggtc gacacggcgg cgaggccgac gcccacggtc atgaacggcc tggcggtgcg
     1381 ccggcgcgcc ggctgctcga tgaagcgcca cgacagatat gccaaaccga tgcatgccgc
     1441 ggccaagcca gtagcgacca ggaaagtggg cgcggcgcct gacgcgtagt ggcggtaaaa
     1501 cacgagcacc ggccaatgcc agaggtatag cgaataggaa atcagcccaa tgaaccgcat
     1561 tgccggtacg gatagcatct gagcgacgag ggtctcgctt gcgcgcggcc agatgatcaa
     1621 agcggcgccg acgcacgcgg gcatggcgtt cagtccaggg aacgagtgtt cgcgcgagag
     1681 cgtaagaacg ctgatggcga tcagcgcgac gccgatgatt gtgggcagct cgtttttcca
     1741 tctgctcgct gattttcgca gcggcggcag gaacacgagc aacgctccaa gcgcgagctc
     1801 ccatgcgcga aggtgggcga ggaaaaaggc ggacttcggg tcggtgccgg ttcgctgaac
     1861 ggccagccat aggccgccga ttacaaccgc gccgacgaga gccgcaacga cgacggcttg
     1921 tttgcgagcg atgaagaaga acgctgccag gagtagaggc cagacgagat agaactgttc
     1981 ttcaaccgac agcgaccaca ggtgcaatag cggcagcagg tcggcggcac gatcgaaata
     2041 tccggtgttc tgcaggaagt accagttggc gagcgaagcg gcggaatatg ccgcgctgtc
     2101 cccgagcgag gtgtagtcgc cgggcagaag aaagaagtag ccggcgacca atgacgcggc
     2161 cagcaccacc aacattgccg gaaatattcg acgaatgcgt cgctggtaaa acgccagaag
     2221 actgaaaccg ccggccttga gatcggctac gatcagcgac gtgatcaaaa agcccgatat
     2281 gacgaagaag atatcgacac cggtaaagcc gcctgataac gcgctgacga tgccgaagtg
     2341 atagagcagg acgctcatca ccgcgaccgc gcgcagccca tcgatgtccg ctctgtaaga
     2401 cagctgtgtc acgatgatag agcccgcgat ccaacgtcca ctcttatcat gactcgtctc
     2461 gcctcgaaat agtctcccat gttgtttggc ggcatcaagg cgttgctgca gcgaggcgcc
     2521 ttcggtcaag tcagtcgcgg tgaaactcgg cggccgcgta cgatcttgca gacatggcca
     2581 aagccatgct tggagccgtg tccgaaaatg gtgacggtat caagatggtt gcgaccctga
     2641 agcgcttcgg atttagccag cgcgtaacat cggctcatgg gaagtccgct ttcgtgccaa
     2701 aacaactcgc gtgtgggcaa agcggaacag ctggcgcagg accggaagca atttgagggg
     2761 gatcgcgagg tgcctggatc gtccagggct gtggtcgaca aacgcgtctt cgacatattc
     2821 gaactcgcca aatgtctgct gcaggagatc cgtgatttga cggcctgaca ggtaattgac
     2881 tccgtgtgtc aggaaatagc gattggcctc caccaattga tcgacggtga aattttcctc
     2941 gtacagatta cccctgatgc caagagcggc gcacagcttc agataaccgg gcgaattgaa
     3001 gacgccgccg aagggaatgt ccgtgtgcgc ttcgatcggc cgccacctcg atggaaacac
     3061 gttgagaaac cagccgccca gcttcaatac ccggtgaatt tccgagaagg ccaatttttg
     3121 ttcgcgcacg tgctcgaaca cggaagtcgc aaacacgaaa tcaaacacat tgtcaggcca
     3181 cggaatcttg ctcatcggcg gatagtcgat cccgtctgcg gagattggat cgaagcggaa
     3241 atgcgcgatg tcggcggcag accgcagatt gacataattc ttgacgtcat aaccgaaggc
     3301 gttgggaaat cccgcctcga gatattcgta ggtgtgccgg ccagaaccac atccgaaatc
     3361 cagtatcatg ctttgcgccg acacgatgcc gccgcgcgac ttgagaatcg cgtgattgag
     3421 gtcaacgacg cgctgagcgg acgggtcttc gatcatcgcg tcatcttacg tacgtcgacg
     3481 agccgcgcgc aagttttcgg agttgacagc catgtgccgt ttcgattgtg tggcgcaaat
     3541 ccgcgggaat gcaccgcgtg cgcttggcat cccgtgcatt tttgccgctc aatcgccagg
     3601 ccgaggcaga gagtccctac aatttgatct cccactcgaa ggcagatgat cgagtcagca
     3661 agtcgatcga cgcaactgcc aattcccaat ccggccgcat cgccgagatg cctttatcaa
     3721 ctggccgggt agggtgagcc gcacgggcat cgcgatgata aaaacgggac tgtatcgcgt
     3781 cgatcagctg tcatcgtctg caaagatcag aggatttttt ggcgggacac gacgggtttc
     3841 gattacgctt tacgagaagc tgcatggaat gaaaaagccc gaagaatggg ccgaaaagat
     3901 cctttttagc cactgcgacg cgcgcggtat ttataagcga acctatgcgt gtcgattcga
     3961 tcagtttgac gatatggcga tcgactgcct cgggcgagag ttccccgcag gccgcgcatt
     4021 gacgatgcac gacatcggcg tgagcgatgg gcgcacagcg tgcgatttct tccaaaagct
     4081 cgctgcgcgg tttccacatc tgaactatta cgcgtcggac tatgagcctt cgctgatgat
     4141 gttgcgttct ggcaagggtg gaagcgttgt gacgctgaac aaaaaagggg aggcaatcga
     4201 gatcgtcatg ccgccgttcg tattcaattt gatcaagcca gagaacttct tgctctatcc
     4261 catcaattat gccttctttt tgttcgctcg cgccatagtg ctccgtcgga cactcgcaaa
     4321 atatcgcgcc ggcaagatcg agccatcgcc tctcgtacta ttctgcccgg cagcgcgaga
     4381 gcttgctgca agcgatgggc gcttccgttt gctggagtac gatctgctgc agcctcacgc
     4441 cctgccgtgt agattggatg cagtccgggt tatgaacgtt ctgaattctt cgtattttga
     4501 tccgcagcag ctggcgattg cggtgaggca cattttcgaa tctttggccc ccggcgggct
     4561 gctgatcatc ggctccaacg acgcggccgg cacccacgtg cgcgggggaa tctatcggaa
     4621 gctgaaccaa ggatttcaaa tgctggccaa gtctgacgcc gatcacgatg ctcaccacac
     4681 catcatgggc tgtactgcga tcgaacgcga cggcctgtag ttccgctggc acgcccgatc
     4741 gctgaccggc aaggccgcca tggcccggct agatcgaggg cttgggtggc aacctcggcg
     4801 acatcgctgg atcgacccgt ccgtattggc ctaagctgcc gggtccgcgg gggctcatcc
     4861 ccgcgggccg gcgctgcttt tcggccccgt cccatgcgcg ccggccgacg aggagtgccc
     4921 atgccattgc ccgtcacctt cgaccactgc gtggtccatg tctcggactg ggagcgctcg
     4981 aacgcattct accgcgatgt gctcggcgcc gagctcgttc ggcgtccgca gggctgggcc
     5041 taccggttcg gcgccgcgca gctcaatttg catgggccgg gctttcaccc ggccgaggtg
     5101 gcgcggcttc cggtcgagcc cggcaacagc gacctctgct tccaatggta cgggccgatt
     5161 gcggacgccg tcacccacct tgagcgctgc cgggtcccga ttcacgccgg gccgatcgag
     5221 cgtttcggcg ccaaggggac cggcatcagc gtctatttcc gcgaccccga cggttcgctg
     5281 ctggaattca tttcctatca ccgcaaggac ggagagcgca cctgacgatc gcgtacgacc
     5341 caagccgccg gccaacagcg gggcattgca ggacgacgac gcagacctcg tcgccatgga
     5401 tggcgctgcg cggcgcgcat gcgacggcaa aggcagaggg cgccacatga gctcccgaga
     5461 ctttccgccc caagacatcc tggtgccgat ctcggtgggc gaactgatgg acaagatcac
     5521 catcctcgaa ataaagtccg agcggctcaa gaatccgagc cagctcgaca atgtcacgca
     5581 ggaactcgcc gccttgcgcg ccgtgcgcct cggcgacgtc gaccgtgtaa cgctcgataa
     5641 gctcggcgcc gagctcaggc gggtgaatgc agaactttgg gacgtcgagg acgcgatccg
     5701 cgaatgcgac gcgcgcggtg acttcggcca atccttcatc gaccttgcgc gcgccgtcta
     5761 ccggctcaac gacgaacgct cgcggctcaa gaaggcgatc aaccttgcgt cagggtcgcg
     5821 cctgatcgag gagaagtcct attcgttcta tcatcccaaa ggcggagacc agccatgacc
     5881 accttgcacg atcccaccgt cttgcccgcc gacatcccgg cgccgcagga tgacggcgca
     5941 gcgcggcatc tcacgggcac aaggcttcct tcgctgccgc tcgcggcgac cgacggctcg
     6001 caggtcgatc tttcgaaact tgccggccgc acggtggtct acatctaccc gcgcaccggc
     6061 gttcccgggc agccgctgcc ggacggctgg aatcagatcc cgggggcgcg cggctgcacg
     6121 ccgcaatcct gcggcttccg cgaccatttc gccgagctca agcagctcgg cgtcgcccgg
     6181 cttttcggcc tctcgaccca ggacaccgcc tatcaacgcg aggcggtcga gcgtctgcat
     6241 ctgccgttcc cggtgctttc ggaccagaac ctcgagctcg cgcgcgcggt caagctgccg
     6301 accttcacga cctcggggat gacgctgctc aagcgcatgg cgctcatcat cgacgacggc
     6361 gtcatcacca aagtgttcta tccggtattc ccgccggaca agaacgccga ggaggtgatc
     6421 gcctggcttc gccgcgcgta gggcgttgag ctcagacgat ggtcgatcac gcccccgcct
     6481 tcgcggggtt gaggggccgt cgatcaatcg ccgaggaagg cgcgcgccgc cgcgaacacg
     6541 tcttcaaaca ttttgggcgt cagcacgccg gtgctggtgt tgtagcgcga gcagtgatag
     6601 ctggcgaaaa tgcgcagccg gccgatttcg tgcgaggcgc catggccgaa cggcgccgcc
     6661 gcgcggcgct ggttgagcgc ggtgagcgtg ctgtcgtggg aaatccgtcc gagcgtcacg
     6721 atggcgcgca gatttcgcat ctcggcgatg gtcgcctgca gaaacgaacg gcaggttttg
     6781 acctcggctg gcgtgggctt gttttcgggc ggcacgcagc gcaccgcatt ggtgatgcgc
     6841 gcatcgacga gcgtgagccc atcgtccgct gaggccagat agctgccgcg ggcaaagccg
     6901 aaacgtttga gcgtgtcgta gaggaggccg ccggcaaagt cgccggtaaa cggccgcccc
     6961 gtgcggtttg cgcccctcag accgggggcc aaacccacga tcagcagccg cgcactgcgc
     7021 ggaccaaacg atgcgaccgg agcattgaac cactgcgcct cgcggcggcg ccactcctcg
     7081 cggaacgctt tgaggcgcgg gcagagcggg caatcgcggc cgggcttgcc ggacgcatcg
     7141 gcgtggcggt gctcagtctt cgaaatcgtc gtcatcgagg ggcgcgcccg cccccgcccg
     7201 ctgcggcgcc gtcgagcgct gcaggaattg cggggtgtgg cgctcgcgcg gttcgcgcgc
     7261 ttcgcgcgcg gcgcgctcgg cggggtcgcg gccgatcttg ggctgcagct cgacgatgtc
     7321 gaggaaaatg tcggcttggc ggcgcaactc gtcggcgacc atcggcggct gggtcgagat
     7381 ggtggagacc acggtcacgc gcacgccgcg ccgctgcacc gcctcgaggc ccgttgcggg
     7441 ggatcttagg ccggctattt cacaggcagg cctcacgccg aggtcgccgg agcccgcctt
     7501 tggtcgcgat gatcaagcct ttaggataag ggtagagcgt ttcggcaatg aaccgctcgt
     7561 tgacctccgg accgtaggcg tcggcggtat cgatgagagt gatgccgagc tcgacagcac
     7621 gcatcagcac gcgcttagca cccggacgat caagcggttc accccataca ccagggccgc
     7681 acaggcgcat ggcgccgaac ccgaggcgat tcacggtcgt gtcgccaccg agggtgaggc
     7741 tgccaccagg acaattcgtc cgcagccg
//
LOCUS       DATLJT010000869         1726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2584927,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000869 DATLJT010000000
VERSION     DATLJT010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584927"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VM910_19990"
     CDS             complement(<1..974)
                     /locus_tag="VM910_19990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HVI64841.1"
                     /translation="MSTGTSSALNSEQDLASIPMAGGGLSRLAIARLKSAGVEVAPLL
                     KRAGLTPEAIAEPEERLSVRSQIALLDEAAIALKDDCIGFTLARDFDPREIGLLYYVM
                     ASSQTLGDGLKRIARYSKITNEALVFGYQEGNRLVLSLGYSGVSRHSDRHQIEFCMFG
                     VLRVCRMLTGQNLVPQHFSISHYRSEGTSEMARFVGTKVEFGADRDEFTLNVDARELP
                     LIHADSYLNDLLLKYCEAALAGRRDDMSQLRTRVENAISSVLPHGKVRVEDVARSLGM
                     SKRTLARRLSDEGLNFTEILQQLRRDLAVRYLDDRKLHVSKIAWLLGF"
     gene            1118..1297
                     /locus_tag="VM910_19995"
     CDS             1118..1297
                     /locus_tag="VM910_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64842.1"
                     /translation="MTLQEAKSIARHLGLTLRKVRSGDYRVSCREGDETTAYYTQNLE
                     DAVNAAVEIARKRAP"
     gene            1414..1713
                     /locus_tag="VM910_20000"
     CDS             1414..1713
                     /locus_tag="VM910_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64843.1"
                     /translation="MPYHSSRIRDWNENDVIAIALTTASISVAAVLDDLKRRLPRALD
                     PLGGDAMTTKEAFEIVLALAFDNLVDEDEMPEIYKQQSEALEIVTEIAQYYGSRR"
BASE COUNT          398 a          513 c          487 g          328 t
ORIGIN      
        1 tgaaaaccga gcagccacgc gatcttggaa acatgcagct tgcgatcatc gaggtaacga
       61 actgccagat cgcggcgcag ttgctgaagt atttcagtga agttcaaccc ttcgtcggag
      121 agccttcgtg ccagcgtcct cttgctcatt cccagactgc gggcaacgtc ttccacgcgc
      181 acctttccat gcggaagcac cgacgagatc gcgttctcaa cccgggtgcg caactggctc
      241 atgtcatctc ttcgaccggc tagcgccgct tcgcaatatt tcaggagaag atcattgaga
      301 tagctgtcgg cgtgaataag cggcaactca cgggcgtcaa cgtttagggt aaactcgtct
      361 ctgtcagcgc cgaactccac tttcgttccg acgaatcgcg ccatttcgga agtgccttcc
      421 gagcggtaat gggaaatcga gaaatgttgc ggtacgaggt tctgccccgt caacatgcga
      481 caaactcgta atactccaaa catgcaaaat tcaatttgat gtctgtccga gtgccgtgaa
      541 acgccggagt aacccagact gagaacgagc ctgttgccct cttgatatcc gaacacgagg
      601 gcctcgttgg taatcttgct gtagcgagca atgcgcttga gtccatcgcc taaggtctgt
      661 gaagacgcca tgacgtaata aagcagcccg atttcgcgcg gatcgaaatc gcgagccagc
      721 gtgaagccaa tacagtcatc cttcagagca atcgccgcct cgtccaaaag cgcgatttgg
      781 cttcgcacac tcagccgctc ttccggttcg gcgattgcct ccggcgtcag tcccgcacgc
      841 ttaagaagag gcgccacctc cacgccggcg cttttgaggc gcgcgatcgc cagcctgctg
      901 aggccgcctc ccgccatcgg gatcgaagcc aagtcctgtt cagaattcag ggctgaagag
      961 gtgccagtgc tcatccttga ccgcccggca ggggcaattg ccgccatgtc cgcgaggaac
     1021 aaatgtttgg cgcaaagtaa caagtcccgc gcggggcgca aagcgactaa gacgcggaac
     1081 cgccattgcg cgggtatcga cagggtattg atgcgccatg acgctgcagg aagccaaatc
     1141 gatcgcgcga catctcgggc tcaccctgcg caaggtgcgc tccggcgact atcgcgtgag
     1201 ctgccgcgag ggagacgaga ccacggccta ctacacgcag aatctcgaag acgccgtcaa
     1261 tgccgcggtg gagatcgccc gtaagcgagc gccgtgagct gcaccatacg gagttggcag
     1321 gtgaaatccg gcaaccaagc tgttctcgat gctcgagggc gctctttcga acgcaaacaa
     1381 ccgaacgccg agcgaggcag gcggtgcaaa gccatgccgt accacagctc ccgcataaga
     1441 gactggaatg agaatgatgt cattgcaatc gcactcacga cggcaagcat ctcggttgcc
     1501 gccgttctgg atgatctcaa acggcgcctc ccgcgcgcgc tggacccatt gggaggcgac
     1561 gccatgacga cgaaagaggc ttttgagatc gtgctggcgt tggccttcga caatctggtc
     1621 gatgaagacg aaatgcctga gatatacaag cagcaatccg aagcgctcga gatcgtcacg
     1681 gaaatagccc aatactacgg ctccaggcgt tgatgacgcc ggcccc
//
LOCUS       DATLJT010000870         4107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_56998,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000870 DATLJT010000000
VERSION     DATLJT010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56998"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            548..715
                     /locus_tag="VM910_20005"
     CDS             548..715
                     /locus_tag="VM910_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64844.1"
                     /translation="MQRVDLWPALTLLLMANPQREIEQRAKAVAPGAPLPEKVHSFID
                     HLSEWFRKHPL"
     gene            754..1737
                     /locus_tag="VM910_20010"
     CDS             754..1737
                     /locus_tag="VM910_20010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64845.1"
                     /translation="MARLRIGLSALLPIVLVASSVVVFQSAQAYPDRPIKLVVPSPAG
                     GPPDQIARLISDKMAAALGQPVIVENRTGGAGGIIGAKSVAAAEPDGHTLFMGSTSSI
                     LIAPLIYKNAGYTAATFAPVARVAESSEMLALHPSMPANTVGELVRLAKSRPGALSYG
                     SAGVGTLPHLEGEFLKTAAQIEMVHVPYRGGGQALTGLLAGEVQLLFSTLVQILPHVR
                     DSRLRALAVTGEKRSPLALDVPTMVESGFDQLITTSVNVIVAPPGTPLQIRRQINQAA
                     AAALASPEVQQAFAILGYEVRAASPDDVASYLAEAQHRWSRLIEAARISID"
     gene            complement(1757..2962)
                     /locus_tag="VM910_20015"
     CDS             complement(1757..2962)
                     /locus_tag="VM910_20015"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI64846.1"
                     /translation="MSSGYRIAIVGGGLAGLAAAHALKAFGMQPEVFEAAPALGEIGA
                     AVNVSPQAFKALQAIGLGDKVAAVATASPGIYTRNMQTGEFLEFNDRHKAAARYGAPY
                     YSFHRADLLDALASGLDRHAIYLGHRLTGLEERSDCIILTFANGAQAEAEVVIGADGV
                     RSVIRHSLYGDDNPTYTGQMVWRALLNADDVPEEVLEPTGHIQWVGPGCHLLAYYIRG
                     KRLVNIVTQEDTDKWVEEGWSIRGDPDEMRMSFPNPEPRLDKLLSIVTECAKWGLFTR
                     PLTQNWGRGRIELIGDAAHAMLPNAGQGACQAFEDAYILARWLEACRDPIEAFANFRR
                     IRIPRVHGVQRLSSANARFKHMHDSAAQKKKIATGMGSVHGNSDWVWGYDPVGGWDKE
                     PFVPTGYAA"
     gene            3125..4078
                     /locus_tag="VM910_20020"
     CDS             3125..4078
                     /locus_tag="VM910_20020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64847.1"
                     /translation="MKCSLVALVAVVAIAAPAHAQTFPNKTIRIIVPYPAGGSIDLTA
                     RVIAKNLQDSVGQNVIVENKPGANAAIGIEALLRSDPDGHTLAILSDSPVTINVHLSR
                     LNYDPLTDLVPITKIVSSPIILAANAKAGIASIADLVAAAKAKPLSYAVAARGSASHL
                     AAELLQRELGITMQAVPYRGGAPAAVAIASGEVPLGLFDTASILPMISSGQVVALGVA
                     EPVRSHSMPNIPTLRESGVPNFSAPSWLALFAPRSTPDDRVIRLNAEIAKIAAMPEAQ
                     KVLFVAGLEPAPNSPSEMRSIIAADYKKWGQLIEATGLKAE"
BASE COUNT          783 a         1299 c         1245 g          780 t
ORIGIN      
        1 acacgcccag cgtcgcatac actccaatga tcatcatcag atagtgcgaa tgtccctgtc
       61 cccaagccca gcccgaaggc cagaaaacac ccaatgtata tatgccaaat atgaaagtta
      121 gaccgatgat gatcagcgct atgcgaagaa aaagcagacg atttgcatca gacatttttg
      181 catctcccaa tggttggcag cttgcccgcc tgctgcgcgc gcgccgcgag tggccacgcg
      241 gccgcgccgc cgagcagcgt gatgaagtcg cgccgtccca attaccaggt ccggattgca
      301 ggatatcgaa ttggcaatcg tcagtcagcg ggtatccggg cccgcacacg tgcgctcaag
      361 gcccagaaat gaggaagcag caggatctct atgtcgtcgc agaccttagc gaagtcgtcg
      421 catagctctt cgctgctcgg atagttcttt atgatttcaa aagtggcgcc gctccaattg
      481 agaccttgca accgaactcg tacggcgcac gtgccttgcc tcggccgatg cactcgactt
      541 cgggcgcgtg caacgagtag acctttggcc cgcgctgacg ctgctcctga tggcgaaccc
      601 gcagcgcgag atcgagcagc gggccaaagc ggttgcgcca ggcgctccgc tgcctgagaa
      661 ggtccacagc ttcatcgacc acctgtcaga atggtttcgc aagcatccat tgtgacgcta
      721 cactcgctcg gagcgatctg tcggaggagg atcatggctc gtttgagaat cgggctttcc
      781 gcccttctgc ccatcgtctt ggtcgcatct agcgtagtgg tctttcaaag cgcgcaagcc
      841 tatccggatc ggcccatcaa acttgtcgtg ccgtcgccgg cgggtggccc cccggaccag
      901 atcgcgcggc tcatctccga caagatggcc gccgcgctcg ggcagccggt gatcgtggag
      961 aaccgaaccg gcggagccgg cgggatcatc ggcgcgaaat cggtcgcagc ggcggagccg
     1021 gacggccaca cgcttttcat gggcagcacg agttccattc tcatcgcgcc gttgatctac
     1081 aagaatgccg gttacaccgc cgcaaccttc gcgcccgtcg ctcgtgtggc ggagagcagc
     1141 gaaatgctcg cgctgcatcc ttcgatgccg gccaacacgg tgggggaatt ggtcagactg
     1201 gcaaagtcgc ggccgggtgc gctgagctac ggctcggccg gcgtcggcac acttccgcat
     1261 ctcgagggcg aattcctcaa aacggcggcc cagatcgaga tggtccatgt tccctatcgc
     1321 ggcggcggtc aggcgctgac gggcctgttg gctggcgagg ttcaactcct cttttcgacg
     1381 ctggtgcaaa tccttccgca tgttcgcgac agccggctca gagctttggc cgtgaccggg
     1441 gaaaagcgca gcccgctggc gcttgacgtt ccgaccatgg tggagagcgg gttcgatcag
     1501 ttgatcacaa cttcggtcaa cgtgatcgtt gcgccacccg gcaccccatt gcagatcaga
     1561 cggcagatca accaggccgc tgctgcggcg ctcgcctcac ccgaggtcca gcaagccttt
     1621 gcgatattgg gctacgaggt gcgagcggcc tcgcccgatg acgttgcgtc ctatttggcc
     1681 gaggcgcagc accgctggtc gcgtctgatc gaggcggccc ggatctcgat cgattgatgc
     1741 tccagtctat ccagcctcat gccgcatatc cggtgggaac aaaaggctcc ttgtcccatc
     1801 cgccaacggg atcgtagccc cagacccagt cggaattgcc atgcacgctg cccatgccgg
     1861 tggcgatctt cttcttctgc gccgcgctgt catgcatgtg cttgaagcgg gcattggccg
     1921 acgagagccg ctgcacccca tgcacgcgcg ggattcggat gcggcggaaa ttggcaaatg
     1981 cctcgatcgg atcgcggcac gcctcgagcc agcgcgcgag aatgtaggcg tcctcgaaag
     2041 cctgacaggc gccttgcccg gcattgggca gcatggcatg cgcggcgtcg ccgatgagtt
     2101 cgatgcgacc gcggccccaa ttctgcgtga ggggccgcgt gaacagaccc cattttgcgc
     2161 actcggtcac gatgctcaaa agcttatcta ggcgcggctc gggattggga aagctcatgc
     2221 gcatctcgtc gggatcgccg cggatggacc agccttcctc cacccatttg tcggtgtctt
     2281 cttgggtgac gatgttcacg agcctcttgc cccggatgta ataggcgagg agatggcaac
     2341 ccggcccgac ccattggata tggcccgtgg gctcgagcac ctcctcgggg acatcgtcgg
     2401 cgttgaggag cgcacgccac accatctggc cggtataggt cggattgtcg tcgccataga
     2461 gcgagtggcg gatgaccgag cgcacgccat cggcaccgat gacgacctcc gcctcggctt
     2521 gagcgccatt agcgaaggtg aggatgatgc agtcgcttcg ctcctccagt cccgtcaggc
     2581 ggtggcccag ataaatggcg tgacgatcga gcccgctggc cagcgcgtcg agcagatcgg
     2641 cgcggtgaaa gctgtaatag ggagcgccat accgcgccgc ggccttatgc cgatcgttga
     2701 attcgaggaa ctcgccggtt tgcatgttgc gcgtgtagat gccgggcgat gcggtcgcga
     2761 ccgcggcgac cttgtctccg agaccgatcg cttgcagcgc tttgaaggct tgcggactga
     2821 cgttgaccgc ggcaccgatc tcgcccaaag ctggcgcggc ctcgaagacc tccggctgca
     2881 tgccaaaagc ctttagcgca tgcgcagcgg cgagcccggc gagcccgccg ccgacaatcg
     2941 cgatccgata gcctgacgac attcgtccct ccacacccct ggccgccacc gtcccaacac
     3001 gactgctgga gcatagcgcg tagctcggat ggagcgaagc taatccgagc tttctgggct
     3061 tgctccttct gccgcgttcc gtccatcatc ggagcggacg ccccgacgtc gaaggcaaat
     3121 attaatgaag tgttcgttgg tcgctttggt cgctgtcgtc gccatcgccg caccggcgca
     3181 tgcgcagaca ttccccaaca agacaatccg aattatcgtg ccttatcccg ccggcggcag
     3241 catcgatctg actgcgcgcg ttatcgccaa gaacctgcag gatagcgttg gacagaacgt
     3301 catcgtggaa aacaaaccag gtgcgaatgc ggccatcggc atcgaagccc tgctgcgaag
     3361 cgatccggac ggccatacgc tcgctatcct ttcggatagt ccagtcacga tcaacgtgca
     3421 tctgtcgcga ctcaattacg atcctcttac cgacttggtg ccgattacga agatagtgag
     3481 cagcccaatc atcttggccg ccaacgccaa ggcggggata gcatcgattg ccgatcttgt
     3541 agcggccgcg aaagccaagc ccttgagcta cgccgttgcg gctcgcggct cggcgtccca
     3601 cctggcggct gagttgctcc agcgtgaact cgggatcacg atgcaagccg taccctatcg
     3661 cggaggcgca ccggcggcgg ttgcgatcgc ctccggcgag gtccccttgg gattgttcga
     3721 cacggcttcg attttgccga tgatcagtag cggccaggtt gtcgccttgg gtgtggcaga
     3781 gcccgtgcgt tcgcactcca tgccgaacat tccgacgctg cgcgagtccg gcgttcctaa
     3841 cttcagcgcc ccctcgtggc tggcgctatt cgccccgcgc agcacgccgg acgaccgtgt
     3901 cattaggctc aacgccgaga tcgccaagat cgcagcaatg cccgaagcgc aaaaggtcct
     3961 gttcgtcgcg ggcctcgagc ctgcccccaa cagtccgagt gagatgcgca gcatcatcgc
     4021 agcggactac aagaaatggg gtcagttgat agaggcgacg ggcctcaagg ccgagtaatg
     4081 cgaatctatc gaagccagga gtcgaca
//
LOCUS       DATLJT010000871         7085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4192578,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000871 DATLJT010000000
VERSION     DATLJT010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4192578"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VM910_20025"
     CDS             complement(<1..905)
                     /locus_tag="VM910_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI64848.1"
                     /translation="MMSQDQNDLITRIGPKDPCGKLMRMYWQPAALVDELHGPRPVRP
                     VKLLGENLVLFRDEQGRYGLIDRHCAHRGADLAFGRLEHGGLRCSFHGWLYDTTGQCL
                     DTPAEPRGSKLCSGIKQRAYPVIEKSGILWAYLGEGDPPAFPEIDCFIAPESHVFAFK
                     GHWACNWLQALEVGVDPSHASYLHRFFEDEDTSNAYGRQFRGASAGSEMPMTKVMREY
                     DRPIINVEHTEYGMRLITLREIDAERTHVRVTNQLFPHAFVIPMNTEMTITQYHVPID
                     DENCYWYSIFTSYSTPVDKKKMRDQR"
     gene            1039..1824
                     /locus_tag="VM910_20030"
     CDS             1039..1824
                     /locus_tag="VM910_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HVI64849.1"
                     /translation="MVKPKRTDGEPRATDFVESLDRGLRLLRAFGQSTAPMSLSEIAR
                     AADLPRATARRILFTLQHGGYVRCDGKLFALTAHVLTLAGSFLRSNQIVAVLQPVLDA
                     IAVAAQEISSLAVLDGDDVVFVARGSPTRIFSGGLEIGYRLPSFCTSVGRAMLGRLGD
                     AELKQRLAAMRREPLTPQTVIDPKRLFASIVADRERGYSLVDREAEPHFRSISVPVHR
                     YDNVIVGAINIGAHVDRVPARELVDRFLPLLRQGAADVQDKLL"
     gene            complement(1834..2367)
                     /locus_tag="VM910_20035"
     CDS             complement(1834..2367)
                     /locus_tag="VM910_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64850.1"
                     /translation="MKLIFLHGAPAVGKLTVAKALLRLVPGRLMDNHAAIDLARTIFD
                     FGAPGFWEMVHASRCAALQVAGEHDVPLLAMTFCYADPHDQANFADFQAITRLYGGEL
                     LPVFLHCSREEAARRVGNPDRVERRKLTSAEILLKDLDRYNYTSVPRTDCIRFDTEKM
                     PSDTVAREIVNRFELTG"
     gene            complement(2409..3314)
                     /locus_tag="VM910_20040"
     CDS             complement(2409..3314)
                     /locus_tag="VM910_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI64851.1"
                     /translation="MSEAADPESFFLEQLGQRVRTMRALRGMSRKVLAKVSGISERYI
                     AQLESGKGNVSIVLLRRVSNAMGAHLEDLIPSSDPAPDWPVLRDLVRKATPAQIAQAK
                     DVLAGHSTSSHHASFCGIALIGLRGAGKSTLGKILAKRIGWNFVELNKEIEAQNGLSV
                     AEIIALYGQEGFRRMEQSALQQLLARKELMVLATGGGIVSEPLTFDLILSSFYTVWLK
                     AEPEEHMGRVRKQGDLRPMADDRSAMAELRNILLSREPLYSRASAVVDTAGLSVDAAA
                     AKLIDTVRPVLQNEARSFGLRTVAV"
     gene            3406..4944
                     /locus_tag="VM910_20045"
     CDS             3406..4944
                     /locus_tag="VM910_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HVI64852.1"
                     /translation="MTSYNAVTWLLDRNVEEGRGAKLAYTDTVSELTYGALQQQTRRL
                     ANLLRRLGVRREERVAMIMLDTVDFPVTFLGAIRAGIVPVPLNTLLTSEQYAYVLADC
                     RARVLFVSEALFPVVKDMVGRLPDLEHVVVSGKSASGHKLFAEEIARESDSFATVATH
                     ADEPAFWLYSSGSTGMPKGVRHLHANLEATANTYAKQVLGIREDDVCLSAAKLFFAYG
                     LGNAMTFPMSVGATTVLNAERSTPALMFALMNKYNPTIFYGVPTLFAAMLNDETVKHE
                     RCGNRLRVCTSAGEALPESVGNSWKARFGVDILDGVGSTELLHIFLSNAPGDIKYGSS
                     GRPVPGYEVRLVNENGGDVADGEVGEMLVHAPSAGEGYWNQRSKSRSTFEGYWTRTGD
                     KYVRDADGRYTFCGRSDDMFKVSGIWVSPFEVESALITHPSVLEAAVVPKADPEGLLK
                     PKAFVVLRPEAKRDGLDETLKDYVKQKIGVWKYPRWIETVDALPKTATGKIQRFKLRD
                     NPRH"
     gene            4995..5810
                     /locus_tag="VM910_20050"
     CDS             4995..5810
                     /locus_tag="VM910_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI64853.1"
                     /translation="MTLLSTGFLRVGMSELEYRMIGPAPDKAPTIVMLHEGLGSAALW
                     GDFPEQLQSATGAGVFVYSRAGYGASSPVKLPRPLDYMQIEALDVLPQLLEQIDFRHG
                     LLVGHSDGASIAAIYAGGTGDHRVRGVALIAPHFIVEDISVASIAEIKTAYETTDLRA
                     KLARWHKDVDNAFYGWNGAWLDPKFRNWDISDYLAYIRVPVAVIQGADDQYGTIRQVA
                     IAQEECYCPVDVTIIAGAGHQPHREAPAATLDAISDFANAVLRTHGGFRGRAA"
     gene            5853..>7085
                     /gene="boxB"
                     /locus_tag="VM910_20055"
     CDS             5853..>7085
                     /gene="boxB"
                     /locus_tag="VM910_20055"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HVI64854.1"
                     /translation="MNANIMNVDYTTKIPNNVNLAADRQVLKALEGWHPGYIDWWNDM
                     GPEGFQQSLVYLRTAYSVDPRGWAKFDYVRMPDYRWGILLAPQEENRVIPFGEHYGEP
                     AWQEVPGEHRAMLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDMRNLFQVNVEEGRHL
                     WAMVYLLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEKTPDWLSFFMFTYFTDR
                     DGKMQLHSLAQSGFDPLSRTCRFMLTEEAHHMFVGETGITRVVQRTCDAMREAGIDDP
                     TDVAKVRALGVIDLPTIQKKLNLHYSLSLDLFGSEVSTNAANAFNAGIKGRYRETQIE
                     DDHRLQNSTYPVLKLVDGEIKPVDEPALTALNMRLRDDYTQDCVKGMLRWNKVISTAG
                     YDYRLTLPHVAFHRDIGEF"
BASE COUNT         1276 a         2211 c         2255 g         1343 t
ORIGIN      
        1 cgctggtcgc gcatcttctt cttgtcgacc ggcgtcgagt aggaggtgaa gatcgagtac
       61 cagtagcagt tctcgtcgtc gatcggcacg tggtactgcg tgatcgtcat ctcggtgttc
      121 atggggatga caaaagcgtg cgggaagagc tggttggtga cgcgcacatg agtgcgctcg
      181 gcgtcgatct cgcgcagcgt gatcagccgc atcccgtatt cggtgtgctc gacattgatg
      241 atgggacggt cgtattcgcg catcaccttg gtcatcggca tttcgctgcc ggcggacgcg
      301 ccgcggaact ggcggccgta cgcgttcgac gtatcctcgt cctcgaagaa gcgatgcagg
      361 taggacgcgt gcgaaggatc gacgccgact tcgagcgcct gcagccagtt gcaggcccaa
      421 tggcccttga atgcgaagac gtggctctcg ggcgcaatga agcagtcgat ctccggaaag
      481 gccggcgggt cgccttcgcc aagataagcc cagaggatgc cgctcttctc gatcacggga
      541 taagcgcgct gcttgatccc ggagcagagc tttgagcccc tgggctcggc tggtgtgtcg
      601 aggcactggc cggtcgtgtc gtaaagccag ccatgaaatg agcagcgcag gccgccatgc
      661 tccagccggc caaaggcgag gtcggcgccc cgatgcgcgc agtggcgatc gatcaggccg
      721 taacggcctt gctcgtcgcg gaacagcacc aggttctccc cgagcagttt cacgggccgc
      781 accggccgcg ggccgtgcag ttcgtcgacc agcgccgccg gctgccaata catccgcatc
      841 agttttccgc aggggtcctt gggcccgatg cgggtgatca ggtcgttctg gtcctggctc
      901 atcatggcgg cgtcacctcg cgttacgtga tcggacttca tggggcttgc gagtgcaagc
      961 ggttctggct atcctgttcg tctattgaac atatgttcaa tatcgtaaca cgagccaaaa
     1021 acttcgcaag cgactttcat ggtcaagcca aagcgcaccg acggtgagcc ccgcgccacc
     1081 gactttgtcg aaagcctcga tcgcggcctg cggttgttgc gagccttcgg ccagagcacg
     1141 gcgccgatga gcctgagcga gatcgcccgt gccgccgatc tgccccgcgc cactgcgcgg
     1201 cggattctct ttaccctgca gcacggcggt tatgtcaggt gcgacggcaa gctgtttgcg
     1261 ctgaccgcgc atgtgctgac gcttgcgggc agctttctgc gctcgaacca gatcgttgcg
     1321 gtgctgcagc cggtgctcga cgcaatcgca gtcgcggccc aggagatctc gtcgcttgcc
     1381 gtgctcgacg gcgatgatgt cgtgttcgtt gcccgcggca gtccgacgcg gattttttcc
     1441 ggcgggctcg agatcggcta tcggctgccc tccttctgca cctcggtcgg ccgcgccatg
     1501 ctcgggcggt tgggcgatgc cgaattgaag caaaggctgg cggcgatgcg gcgcgaaccg
     1561 ctgacgccgc agaccgtgat cgatccgaag cgcttgtttg cttccatcgt tgccgaccgc
     1621 gagcgcggct attcgctggt cgatcgcgag gccgagccgc atttccgctc gatctcggtg
     1681 ccggtgcacc gctacgacaa cgtcatcgtc ggcgcgatca acatcggcgc gcatgtcgac
     1741 cgcgtacccg cgcgcgaact ggtcgaccgc ttcctgccgc ttttgcgaca gggtgcggcg
     1801 gatgtacagg acaagctgct ttgaaccgca gcctcagccc gtaagctcga atcggttgac
     1861 gatttcccgc gcgaccgtgt cagacggcat cttttcggta tcgaatctga tgcagtcggt
     1921 ccgcggcacc gatgtgtaat tgtagcgatc gaggtccttg agcaggattt ccgccgacgt
     1981 cagcttgcgc cgctcgaccc ggtcggggtt gccgactcgc cgtgcggcct cctcgcgcga
     2041 gcagtgcagg aacaccggaa gcaattcgcc gccatacagg cgggtaattg cctggaagtc
     2101 cgcaaagttg gcctggtcat gcggatcggc gtagcagaac gtcatcgcca gcaatggcac
     2161 gtcgtgttcc cccgccactt gcagcgcagc gcagcgggaa gcgtgcacca tctcccagaa
     2221 tcccggcgcg ccgaaatcga agatagtgcg ggcgagatcg atggctgcgt gattgtccat
     2281 cagccggccc ggcacaagac gcagcagcgc cttggccacc gtcagtttgc caacagccgg
     2341 cgcgccatgg aggaagatca gtttcatggg ccgcagtata accggcatag ccggccagcc
     2401 ggcaagcgct acaccgcaac cgttcgcagc ccgaacgacc gcgcctcgtt ctgcagcacg
     2461 ggcctcacgg tgtcgatcag cttcgccgcg gcggcatcga ccgaaagacc ggcggtgtcg
     2521 accacggcag atgcgcgcga atacagcggc tctcggctca agaggatgtt gcgcaactcg
     2581 gccatcgcag aacggtcgtc ggccatcggg cgcaaatcgc cttgcttgcg cacgcggccc
     2641 atgtgctcct ccggctcggc cttgagccac accgtgtaga acgacgacag gatcagatcg
     2701 aaggtgaggg gttcggagac gatgccgccg ccagtcgcga gcaccatcaa ttccttgcgc
     2761 gccagcagtt gctgaagcgc gctctgctcc atgcggcgaa agccctcctg cccgtagagt
     2821 gcgatgatct ccgcgaccga caatccgttc tgcgcttcga tctccttgtt cagctcgacg
     2881 aaattccagc cgatccgttt cgcgaggatt ttccccagcg tcgatttgcc ggcgccgcgc
     2941 agtccgatca gcgcgatgcc gcagaacgac gcgtgatgcg atgatgtgct gtgaccggcg
     3001 agcacgtctt ttgcctgcgc gatctgggcg ggcgttgcct tgcgcacgag gtcccgaagc
     3061 acgggccagt ccggcgccgg atccgatgac ggaatgaggt cttcgagatg cgcgcccatc
     3121 gcgttcgaga cgcggcgcag cagcacgatc gagacattgc ccttgccgct ttcgagctgc
     3181 gcgatgtagc gctcggaaat tccagacacc ttggcgagca ccttccgcga catgccgcgc
     3241 agcgcgcgca tggtgcggac gcgctgccca agctgttcga gaaagaagga ttcgggatcg
     3301 gcagcttcac tcatgttccg gaaacataat gccgaagagg attgacagca agcgcacttg
     3361 gtgcctttct atgaattata attcgcaaat gcggggagaa acgtcgtgac ttcctacaat
     3421 gcggtgacct ggttgctcga ccgcaatgtc gaggaggggc gcggtgccaa gcttgcctat
     3481 accgacacgg tctccgaact gacctatggc gccctgcagc aacagacccg gcggctcgcc
     3541 aatctgttgc gccgtcttgg cgtccgccgc gaggagcggg tcgccatgat catgctcgat
     3601 accgtggatt tcccggtcac ctttctcggc gcgatccgcg ccggcatcgt gccggtgccg
     3661 ctgaatacgc tgctcacgtc cgagcaatat gcctacgtgc tggcggattg ccgggcgcgc
     3721 gtgctgttcg tctccgaggc gctgtttccc gtcgtgaagg acatggtcgg ccgcttgccc
     3781 gacctcgaac atgtcgtggt gtcgggcaag agcgcttccg gccacaagct gttcgccgag
     3841 gagatcgcgc gcgagagcga cagctttgct accgtggcga cgcatgccga cgagccggct
     3901 ttctggctct attcatcggg ctcgaccggc atgcccaagg gtgtgcggca tctgcacgcc
     3961 aatctcgaag ctaccgccaa cacctacgcg aaacaggtgc tcggcatccg cgaggacgat
     4021 gtctgcctgt ccgcggcaaa actgttcttc gcctacggtc tcggcaatgc gatgactttt
     4081 ccgatgtcgg tcggtgcgac cacggttctc aatgcggagc gatcgacgcc ggcgctgatg
     4141 ttcgcgctga tgaacaaata caacccgacc attttctacg gcgtgccgac gctgtttgcc
     4201 gccatgctca acgatgagac ggtgaaacat gagcgctgcg gcaaccggct gcgcgtctgt
     4261 acctcggccg gcgaggcgtt gccggaatcg gtcggtaata gctggaaggc gcgttttggc
     4321 gtcgacatcc tcgacggcgt cggctcgacc gagctgttgc atatcttcct gtcgaacgcg
     4381 cccggcgaca tcaaatacgg ctcctcggga cgtccggtgc caggctacga ggtgcggctc
     4441 gtgaacgaga acggtggcga cgtcgccgat ggcgaggtcg gcgagatgct ggtgcatgcg
     4501 ccgtccgcgg gagaaggtta ctggaatcag cgctcgaaga gccgttcgac gttcgaaggc
     4561 tattggaccc gaaccggcga caaatatgtg cgcgatgccg acgggcgcta caccttctgc
     4621 ggccgcagcg acgacatgtt caaagtatcg ggcatctggg tctcgccgtt cgaggtggag
     4681 agcgcgctga ttacccatcc gtcggtgctg gaggccgccg tcgtgccgaa ggccgatccc
     4741 gaaggcctgc tcaagccgaa ggcgttcgtc gtgctgcgtc cggaggccaa gcgagacggg
     4801 ctcgacgaga cgttgaagga ctacgtcaag cagaagatcg gcgtttggaa atatccgcgt
     4861 tggatcgaga cggtcgatgc cttgccaaaa accgcaaccg gaaaaatcca gcgcttcaag
     4921 ctgcgggaca atccacgtca ctagccgtca tgccatggcc gggtcaggcc cggccatgac
     4981 gagaggaaaa atgcatgacc cttttgtcca caggctttct gcgcgtcggc atgtccgaac
     5041 tcgaataccg catgatcggc cctgcgcccg acaaggcgcc gaccatcgtc atgctgcatg
     5101 agggattggg ttcggccgcg ctgtggggcg acttccccga gcagttgcag agcgcgaccg
     5161 gcgccggcgt gttcgtctat tcgcgcgccg gttatggtgc atcgagcccg gtcaagctgc
     5221 cgcgcccgct cgactatatg cagatcgagg cgctcgacgt gctgccgcaa ttgctcgagc
     5281 aaatcgactt tcgtcacggt cttttggtcg ggcactccga cggcgcctcg attgcggcga
     5341 tctatgccgg cggcaccggg gatcaccgcg tgcgcggcgt tgcgctgatt gcaccgcatt
     5401 tcatcgtcga ggatatctcg gttgcgtcca tcgcggagat caaaacggcc tacgagacca
     5461 cggatctgcg cgcaaagctc gcgcgctggc acaaggatgt cgacaacgcc ttttacggct
     5521 ggaacggcgc ctggctcgat ccgaaattcc gcaactggga catttccgat tatctcgcct
     5581 acatccgcgt gccggttgcg gtcatccagg gcgccgacga ccagtatggg acgatccgac
     5641 aggtcgcgat cgcgcaggaa gaatgctact gccccgtcga cgtgacgatc attgccgggg
     5701 ccggacacca gccgcatcgc gaggcgccgg cggcgacgct ggatgcgatc tcggattttg
     5761 ccaacgcggt gttgcggacg cacgggggtt ttcgggggcg ggccgcctga gccgtccgcg
     5821 gttcgacatg acgagagttt gaggagcacg ccatgaacgc caacatcatg aatgtcgatt
     5881 acaccaccaa gattccgaac aacgtgaatc ttgccgccga tcgccaggtg ctcaaggcgc
     5941 tggagggctg gcatcccggt tacatcgact ggtggaacga catgggcccg gagggcttcc
     6001 agcaatcgct ggtctatctg cgcacggcct attcggtcga tccgcgcggc tgggccaagt
     6061 tcgactatgt caggatgccc gactatcgct ggggcattct tcttgcgccg caggaggaaa
     6121 atcgcgtcat ccccttcggc gagcattatg gcgagccggc ctggcaggaa gtgccgggcg
     6181 agcatcgcgc catgctgcgg cggctgatcg tgatccaggg cgacacggag ccggcttccg
     6241 tggagcagca gcgccatctc ggcaagaccg cgccctcgct ctacgacatg cgcaacctgt
     6301 tccaggtcaa tgtcgaggag ggccgccacc tctgggcgat ggtctatctc ctgcagaaat
     6361 attttggccg cgacggccgc gaggaggcgg acgaattgct gcggcgccgc tcgggcgatg
     6421 ccgattcccc gcgcatgctg ggcgccttca acgagaagac gccggactgg ctgtccttct
     6481 tcatgttcac ctacttcacc gaccgtgacg gcaagatgca gttgcactcg ctcgcgcagt
     6541 ccggtttcga tcccttgtcg cgcacctgcc gcttcatgct gacggaagag gcgcaccaca
     6601 tgttcgtcgg cgagaccggg atcacgcgcg tggtgcagcg gacctgcgac gccatgaggg
     6661 aagccggcat cgacgatccg actgacgtcg ccaaggtgcg cgcgctcggc gtgatcgatc
     6721 tgccgaccat ccagaaaaaa ctcaacctgc attattcgct ctctctcgac ctgttcggct
     6781 cggaggtttc gaccaatgcg gcgaacgcgt tcaacgccgg tatcaagggc cgttacaggg
     6841 agacgcagat cgaggacgat caccgcctgc agaactcgac ctatccggtg ctgaagctgg
     6901 tcgacggcga gatcaagccg gtcgacgagc cggcgctcac tgcactcaac atgcgccttc
     6961 gcgacgacta cacgcaggat tgcgtcaagg gcatgctgcg ctggaacaag gtgatctcga
     7021 ccgcgggcta cgactacagg ctgacgctgc cgcatgtcgc cttccatcgc gacatcggcg
     7081 agttc
//
LOCUS       DATLJT010000872         6766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3043697,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000872 DATLJT010000000
VERSION     DATLJT010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043697"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VM910_20060"
     CDS             <1..1407
                     /locus_tag="VM910_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HVI64855.1"
                     /translation="VLKADGLKPGDRIAVFLSQSLELPIAHFAAFRSGMISIPLFALF
                     GEDALEFRLSNSGARAIVTDESGWEKVAKIRDRLPELANVYVIGERAPAGAKSFWDSV
                     KAASADFTTIDTSADDPALIIYTSGTTGNPKGALHAHRVVLGHLPNVEMCHDFIPKPG
                     DLMWTPADWAWIGGLVNALFAFWYHGVPMVGHRARKFEPEAAMQMMADLGIRNVFLPP
                     TALKLMRQAGVKHPGIKLRSIFTGGESLGAELLDWVRATFGTDAHEVFGQTECNLVIG
                     SNSKLFPIRPGSMGKATPGFDVRIVNEKGEELPRGERGIIGVRQPNPVTMIEYWRNPD
                     ATRKKYVGDFLLTGDLGVQDQDGYFWYVSREDDVITSAGYRIGPAEIEDTLLKHPAVA
                     LSAVVGIPDPIRTEAVKAWIVLRPGFSPSDQLARDIQNFVKVQLAAHEYPRHVQFTDT
                     LPMTATGKVLRRELRARG"
     gene            1645..2418
                     /locus_tag="VM910_20065"
     CDS             1645..2418
                     /locus_tag="VM910_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HVI64856.1"
                     /translation="MSISGKIDPVVRRISAADIAESLAQGLRDFQALPLYGLAFGALY
                     TAGGILIVLSLTAFGMVYLTYPLAAGFALIGPFVAIGLYEVSRRREAGEPVSFAAIWS
                     TVKSRGEIGWMAFVTLFFFVIWMYQVRLLIALLLGLNASFSSLREFVTMVLTTNEGLL
                     FLAIGNLDGAVLSLILFSLTVVSFPLLLDRDVDFVTAMVTSVRAVVMSPLPMIGWAAV
                     IVMLLIVSVIPYFLGLLVTLPVLGHATWHLYRRIVVPLP"
     gene            complement(2452..2964)
                     /locus_tag="VM910_20070"
     CDS             complement(2452..2964)
                     /locus_tag="VM910_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI64857.1"
                     /translation="MNIDQFKPTLVYTIYIKTTPEKVWQALTSGELSGKYFFGNAVEI
                     DQRIGGAFIVRAPDGSLHISGEVVECERPKKLTVTFNVNWPALIEKLGPTLVTYEIEP
                     AGEAVRLTLTELHDRPLSDDILSGGRSGWPAILSSLKSLLETGEPLVVKLAPPQRMLE
                     ALKALGIGTP"
     gene            complement(2964..3434)
                     /locus_tag="VM910_20075"
     CDS             complement(2964..3434)
                     /locus_tag="VM910_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI64858.1"
                     /translation="MPKPEFVYVTYIKTTPEKLWDALTSSEFSKRYWFDTELRSDWKV
                     GSSFALVMNGTTTDVGEILEANRPRRLSYTFRHVLDDDLRAEPATKVVFTLEPHGDLV
                     KLTLTHEGFADGSKLLDGISKGWPAILSNLKSLLESGRALAIPLAALNIEGVGK"
     gene            complement(3427..3897)
                     /locus_tag="VM910_20080"
     CDS             complement(3427..3897)
                     /locus_tag="VM910_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI64859.1"
                     /translation="MSKPEFVYVTYIKTAPEKLWHALTDSDFTERYWFGNRVTSDWTV
                     GSTYRFAKEGKPGVEGKVLVSDPPKKLSYSWDLVKEGTYRERTSRVTFDLEPHGDVVK
                     LTVTHDDLDEGGKTLRDVSSGWPLVLASLKSLLETGRELPHELAAQARKEQACA"
     gene            complement(3890..4204)
                     /locus_tag="VM910_20085"
     CDS             complement(3890..4204)
                     /locus_tag="VM910_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI64860.1"
                     /translation="MDEVFKALADASRRTLLDRLHARNGQTLNELCDGLDMTRQAVTK
                     HLAILEDANLVTTFKHGREKLHYLNPVPIHQIGERWIRKFERAKLAALSELKKQLEKR
                     DE"
     gene            4328..5776
                     /locus_tag="VM910_20090"
     CDS             4328..5776
                     /locus_tag="VM910_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64861.1"
                     /translation="MSGRVKPVFLLVIPVLACAGFAQAQQQKIGAPPEASNMKLVGYS
                     DLQARSAYQPTIHHQGDRWIAYIGHHGGSDENPDPVNPITGKAEPNGTSIVDVTDPAH
                     PKYLRHIPGQEGKYESGGAQMVRVCDGKSLPKGDPNAVYLLRAIGSEGHEIWNVADPA
                     NPVLVTRIGERLKDTHKNFWECDTGIAYLVSGAPDWRTRRMTQVYDLSDPAHPAKIRD
                     FGLPGQEPGATGPVPTELHGPISLGPQGNRVYFGYGTDKGGILQIVDREKLLNGAKEP
                     TPDNLRYPEIGELPMSAFNGAHTTFPMKGMPIAEFAKDKDGGTRDIVMIVDEAIVNEC
                     NEPRQMVWFADVTTETRPMMISSFTVPESSGNFCERGGRFGAHSSNESMAPVFYKKMA
                     FIAFFNAGVRALDVRDPYHPREVGYFIPAITDKTDKRCVTVEGKERCKIAIQTNNVET
                     DDRGYIYIVDRANTGMHILELTGEARAVAGLP"
     gene            5788..6330
                     /locus_tag="VM910_20095"
     CDS             5788..6330
                     /locus_tag="VM910_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64862.1"
                     /translation="MRRLLIIVAAALAFSALSGVAAYQALPRGEDNAARWQEIAWPFP
                     RDGWPAGRAFRCASCGEAELYVRPKIGFCNCDTGVADDEEVDRVADLDLISARFAPLA
                     PGKIVRGADMSGRSRPYDLAMADGARHVAVGFALSHRCDLVVAVVQGKASTEQLRRAA
                     LQFLESQELHRWIMAGLGER"
     gene            6406..>6766
                     /locus_tag="VM910_20100"
     CDS             6406..>6766
                     /locus_tag="VM910_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVI64863.1"
                     /translation="MSLNVYLAFVAACIALALLPGPIVTLLIANGLRHGTRAALINIL
                     GVQAGLVIAIGVVAVGLTSLMATMGYWFDRVRFAGAAYLVWLGIKLIRAPVEGLSADE
                     LPPPRGGFFLQGFLVALS"
BASE COUNT         1159 a         2078 c         2156 g         1373 t
ORIGIN      
        1 gtgctgaagg cggacgggct caagccgggc gaccgcatcg cggtgttcct gtcgcaatcg
       61 ctggagttgc cgatcgcgca ttttgccgcg ttccgctccg gcatgatttc gattccgttg
      121 tttgcgctgt tcggcgagga cgcactggaa ttccggcttt caaattccgg cgccagggcc
      181 atcgtcaccg acgagagcgg ctgggagaag gtcgccaaaa tccgcgaccg tctgcccgag
      241 cttgccaacg tctatgtcat tggcgagcgc gcgccggccg gcgccaaatc cttctgggat
      301 tccgtcaagg ccgcgtctgc ggactttacg accatcgata cgtctgccga cgatcccgcg
      361 ctgatcattt acacctccgg caccacagga aacccgaagg gtgcgctgca cgcgcaccgc
      421 gtcgtgctcg gccacctgcc gaatgtcgag atgtgccacg atttcatacc caagcccggc
      481 gatctcatgt ggactccggc cgactgggcc tggatcggcg ggctggtcaa cgcgctgttt
      541 gctttctggt atcacggggt gcccatggtc gggcatcgcg cgcgaaagtt cgaacccgag
      601 gcggcgatgc agatgatggc cgatctcggc atccgcaacg tgttcctgcc gccgaccgcc
      661 ctgaagctga tgcggcaggc cggcgtgaag catccgggca tcaagttgcg cagcatcttc
      721 accggcggag aatcgcttgg ggccgaactg ctcgattggg tgcgcgcgac cttcggcacc
      781 gacgcccacg aagtgttcgg ccagaccgaa tgcaatctcg tgatcgggag caattcaaag
      841 ttgtttccga ttcgcccggg ctcgatggga aaggccacgc ccggcttcga cgtgcgcatt
      901 gtcaacgaga agggcgagga attgccgcgg ggcgagcgcg gcatcatcgg cgtgcgccag
      961 ccaaatcccg tcaccatgat cgagtactgg cgcaatcccg atgcaacgcg caagaaatat
     1021 gtcggggatt tccttctgac cggcgacctc ggcgtgcagg accaggacgg ttacttctgg
     1081 tacgtcagcc gcgaggacga cgtcatcacc tctgccggct atcggatcgg accggccgag
     1141 atcgaggaca cgctgctgaa acatccggcc gtcgcgctgt cggcggtggt cggcatcccg
     1201 gatccgatcc gtaccgaagc ggtgaaagca tggatcgtgc tgcggcccgg cttttcgccg
     1261 agcgatcagt tggcccgcga catccagaat tttgtgaagg tgcagctcgc agctcacgaa
     1321 tatccgcgcc atgtccagtt caccgatacg ctgccgatga ccgcgaccgg caaggtgctg
     1381 cggcgcgagt tgcgcgcgcg ggggtagcct gacacaccgc tgtcgtcccc gcgaaagcgg
     1441 ggacccataa ccacaggctc gaattgttgt gcgagatttt agctacgccg ttcttcgcat
     1501 cactccggcc tgtggctatg ggtcccggat cgccctcgct tcgctcggtt gtccgggacg
     1561 acaccaagga tgagaattga gctagatcaa cgcacctcgc tccgcggcct gcgccaatgg
     1621 ccgaaacggc gggaggacgg gctgatgtca atctcaggca agatcgatcc tgtggtgcgg
     1681 cgcatcagcg ccgcggacat tgcggaatcg cttgcgcagg gcctgcggga tttccaggcg
     1741 ctgccgctct acgggctcgc tttcggcgca ctctatacgg ccggtggcat cctgatcgtg
     1801 ctgtctctca ccgccttcgg catggtctat ctgacctatc cgcttgcagc cggcttcgcg
     1861 ctgatcggtc cgttcgtggc gatcggcctc tatgaagtga gccgccgccg cgaggcggga
     1921 gagccggtgt cgtttgcagc gatctggtcc acggtgaagt ctcgcggcga aatcggctgg
     1981 atggcctttg tcacgctgtt cttcttcgtg atctggatgt accaggtgcg gcttctgatc
     2041 gcgctgctgc tcggcctcaa cgcctcattc tcgagtctgc gggaatttgt gacgatggtg
     2101 ctgaccacca atgaggggtt gttattcctt gcaatcggca atctcgacgg cgcggtgctg
     2161 tcgctgattc tgttctcgct gaccgtggtg tcgtttccgc tgctactcga ccgcgacgtc
     2221 gatttcgtca ctgcgatggt gacgagcgtc cgtgccgtgg tgatgtcgcc gctgccgatg
     2281 atcggctggg ccgcggtgat cgtgatgttg ctcattgtct cggtgattcc gtactttctg
     2341 ggtctcctgg tgacgctgcc ggtgctcggc cacgctacct ggcaccttta ccggcgcatc
     2401 gtcgtgccgc tgccctgatt tcggtgcttc cctgggatgc ggggttggtc atcagggcgt
     2461 cccgatcccc agcgctttca gagcctcgag catgcgctgc ggcggggcga gcttgacgac
     2521 cagcggctcg ccggtttcaa gcaggctctt gagactcgag aggatcgcgg gccagcccga
     2581 gcgtccgccg gagaggatgt cgtcgctgag cggccggtcg tgtaactcgg tcagcgtcag
     2641 ccgcaccgcc tcgcccgccg gttcgatctc gtaggtgacg agggtgggac cgagcttctc
     2701 gatcagcgcc ggccagttga cgttgaaggt gacggtgagc tttttcggtc gctcgcattc
     2761 gacgacttcg cccgagatat gcagtgagcc gtcgggcgcg cgcacgatga acgcgccgcc
     2821 gatcctctga tcgatctcga ccgcattgcc gaagaaatat ttgccgctga gctcacccga
     2881 cgtcagcgcc tgccagacct tttccggggt ggtcttgatg tagatcgtgt aaacgagtgt
     2941 cggcttgaac tgatcgatgt tcatcatttc cccacgcctt cgatgttcag cgccgccagc
     3001 gggatcgcca acgctcgccc ggattcgagg aggctcttca ggttggacag gatggcaggc
     3061 catcccttgg agatgccgtc gagcagcttg ctgccatcgg cgaatccttc gtgcgtgagc
     3121 gtcagcttca ccaggtcgcc atgcggctct agggtaaaga cgaccttggt cgccggctcc
     3181 gcacgcagat cgtcatcgag aacatgcctg aaggtgtagg agagccgccg cggccggttg
     3241 gcttcgagaa tctcgccgac atcggtggtg gtgccgttca tgaccaacgc gaacgacgag
     3301 cccaccttcc aatcggatcg cagctcggta tcgaaccagt agcgcttcga aaattcgctc
     3361 gacgtgagcg cgtcccacaa cttctccggc gtcgtcttga tgtaggtgac gtagacgaac
     3421 tcgggcttag gcacaggctt gctccttgcg tgcttgcgcc gcaagctcgt gcggcagttc
     3481 gcgtccggtt tccaggaggc tcttcaggct cgccaagaca agcggccagc cgctggagac
     3541 gtcacgcaag gtcttgccac cctcatcgag atcgtcgtgg gttaccgtca gtttcacgac
     3601 atcgccatgc ggttcgagat cgaaggtgac acgcgaagtg cgctcgcggt aggtgccttc
     3661 cttcaccagg tcccagctat aggaaagctt tttcggtgga tcggagacga gcaccttgcc
     3721 ctcgacgccc ggtttgcctt cttttgcgaa acgataggtc gagccgacgg tccagtcgga
     3781 ggtgacgcgg ttgccgaacc aatagcgttc ggtgaagtcg ctgtcggtca aggcatgcca
     3841 gagcttttca ggcgcggtct tgatgtaggt gacatagacg aattccggct tactcatcgc
     3901 gcttctccag ttgcttcttc agttcgctga gcgcggcaag ctttgcgcgc tcgaatttcc
     3961 tgatccatcg ctcgccgatc tgatggatcg ggacggggtt gagatagtgc agtttctcgc
     4021 ggccatgctt gaacgtcgtg accaggttgg cgtcctccag aatcgcaaga tgcttcgtta
     4081 cggcctggcg cgtcatgtcg aggccgtcgc acagctcgtt cagggtctgt ccgtttctgg
     4141 cgtgaagcct gtccagcaat gtccgccgtg aggcgtcagc cagcgctttg aagacctcat
     4201 ccatggtgca aagtaatatg caactaaatg gttgcatgtc aagagggtgt tttggcggca
     4261 cggtttcgcc gtgctaggtt ttgtccacgc caacgaaaga ttggcctcga gcccgggtgg
     4321 aaaaagcatg agcggtcgcg tcaaaccggt atttctcctc gtcattcccg ttctcgcctg
     4381 cgcaggcttt gcgcaggcgc agcagcaaaa gatcggagcg ccgccggaag cctccaacat
     4441 gaagctggtc ggctatagcg acctgcaggc gcgcagcgcc tatcagccga ccatccatca
     4501 tcagggcgat cgctggattg cctatatcgg tcatcacggc ggctccgacg agaaccccga
     4561 tcccgtcaat cccatcacag gcaaggccga accgaacggc acctcgatcg tcgacgtcac
     4621 cgaccccgcg cacccaaaat atctgcggca cattccggga caggaaggaa aatacgaaag
     4681 cggcggcgcg cagatggtgc gggtctgcga cggcaagtcc ttgccaaagg gcgatccgaa
     4741 cgcggtctat ttgttacgcg cgatcggcag cgagggtcat gaaatctgga atgtcgccga
     4801 tcccgccaac cccgttctgg tcacgcggat cggcgagcgg ctgaaggata ctcacaagaa
     4861 tttctgggaa tgcgacaccg ggatcgcgta cctcgtttcg ggcgcgccgg actggcgcac
     4921 gcgtcgcatg acgcaggtct atgatctctc cgatcccgcg catccggcaa agatcaggga
     4981 ttttggcctg cccggccagg agcccggcgc aaccggcccg gtgccgaccg agctgcacgg
     5041 gccgatctcg ctgggtccgc aaggcaaccg cgtctatttc ggctacggca cggacaaggg
     5101 cggcatcctg cagatcgtcg atcgcgagaa gctactcaac ggagcgaagg agccgacgcc
     5161 ggacaatctg cgttatccgg aaatcgggga gttgccgatg tcggcgttca atggggcgca
     5221 cacgaccttt cccatgaagg gcatgccgat cgccgagttt gcgaaagaca aggacggcgg
     5281 aacgcgcgac atcgtgatga tcgtggacga ggcgattgtg aacgagtgca acgagccgcg
     5341 gcagatggtg tggttcgccg acgtcacgac cgagacgcgg ccgatgatga tttcgagctt
     5401 cacggttccg gaatcgagcg gcaatttctg cgagcgtggc gggcgtttcg gagcgcactc
     5461 gtcaaatgaa agcatggcgc cggtgttcta caagaagatg gcgttcatcg ccttcttcaa
     5521 tgccggcgtg cgcgcgctcg atgtgcgcga cccctatcac cccagggaag tcggctattt
     5581 cattcccgcg atcaccgaca agaccgacaa gcgctgcgtc accgtcgaag gcaaggagcg
     5641 ctgcaagatc gcgatccaga ccaacaatgt cgagaccgac gatcgcggtt acatttatat
     5701 cgtcgatcgt gccaacacag gcatgcacat tctcgaactg accggggaag cgcgcgccgt
     5761 ggcaggtctg ccgtagggag acgcgcgatg cgacgtctgc tgatcatcgt ggctgcggcg
     5821 ctggccttca gcgccttgtc gggcgtcgcc gcctatcagg cgctgccgcg cggcgaggac
     5881 aacgcggcgc gctggcagga gatcgcctgg ccgtttccgc gcgacggctg gccggccggt
     5941 cgcgcgttcc gttgcgcctc ctgcggcgag gccgaacttt atgtgcgacc gaagatcggc
     6001 ttctgcaatt gcgataccgg ggtggccgac gacgaggaag tcgaccgcgt cgccgatctc
     6061 gacctgatca gcgcgcgctt tgcgccgctc gcgccgggca agatcgttcg cggggccgat
     6121 atgtccggcc ggtcgcggcc ttacgatctt gcgatggcgg acggcgcgcg ccacgtcgcg
     6181 gtcggttttg cgctgtcgca tcgctgcgac ctcgtggtcg cggtcgtgca gggcaaagca
     6241 agcacagagc aattgcggcg cgcggcgctg cagtttctgg aatcgcagga gctgcatcgc
     6301 tggatcatgg ccgggctggg cgagcggtag atcgcttgag tgcggcttcc acccgctctt
     6361 ccgtcatgca taatgcgccc gtcgatcccg cccagcgagt tccccatgtc cctcaatgtc
     6421 tatctcgcct tcgtcgccgc ctgcatcgcg cttgcgcttc ttcccggacc gatcgtgacg
     6481 ctgctcattg ccaacgggtt gcgccacggc acccgcgcgg cgctgatcaa tattctgggc
     6541 gtgcaggcgg gactcgtgat cgcaatcggc gttgtcgcgg tcggcctgac ctcgctgatg
     6601 gcgaccatgg gttactggtt cgaccgggtg cgctttgcgg gcgccgccta tctggtctgg
     6661 ctcggcatca agctgatccg cgcgccggtc gagggtttga gcgccgacga gctgccgccg
     6721 ccgcgcggcg gcttcttcct gcagggcttt ctggtcgcgc tgtcga
//
LOCUS       DATLJT010000873        11306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1435541,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000873 DATLJT010000000
VERSION     DATLJT010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435541"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      49..127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            229..1380
                     /gene="gcvT"
                     /locus_tag="VM910_20105"
     CDS             229..1380
                     /gene="gcvT"
                     /locus_tag="VM910_20105"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HVI64864.1"
                     /translation="MSEVHQKAPLLRTPLYKFHAERGARLVAFAGYEMPVQYPTGILS
                     EHLHTRAAVGLFDVSHMGQIELRATSGRLEDASRALETLAPIDIIGIAEGRQRYALFT
                     NAEGGILDDLMVANRGDRLLLVVNAACKTADLAHLRQNLGQTCKIDPLPDRALIALQG
                     PLAEAALARLAPAITTMRFMDVRTLSILGADCEVSRSGYTGEDGFEISIAADRAEAFA
                     RVLCEDHSVMPIGLGARDSLRLEAGLCLYGSDLDPAITPVMASLEWAIQKARRAGGSR
                     AGGFPGADIILRQLTDGAPHRRVGLKVEDRVPVRGGAKLYADEISASTIGIVTSGGFG
                     PSVQAPIAMGYIDENSATTGKRIVGEVRGKRIALQVCDLPFVPHRYKRD"
     gene            1406..1768
                     /gene="gcvH"
                     /locus_tag="VM910_20110"
     CDS             1406..1768
                     /gene="gcvH"
                     /locus_tag="VM910_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVI64865.1"
                     /translation="MLKFTEDHEWLRIEGDVATVGITDHAQEQLGDLVFVELPAPGAR
                     PEQGAGAAVVESVKAASDVYAPVSGEVIEINQAIVDEPALVNRDPMGKGWFFKIKISE
                     PSQLERLLDEKAYQAMIA"
     gene            1818..4721
                     /gene="gcvP"
                     /locus_tag="VM910_20115"
     CDS             1818..4721
                     /gene="gcvP"
                     /locus_tag="VM910_20115"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HVI64866.1"
                     /translation="MPARASAADFNPGDFSRRHIGPSPADIGEMLSTIGASDIGELIS
                     QTVPDAIRQAAPLALPAGISETEALQKLRIIADKNKIFTSLIGQGYYGTILPPVIQRN
                     VLENPAWYTAYTPYQPEISQGRLEALLNFQTMISELCMLDIANASLLDEATAAAEAMA
                     MARRVVKSRANAFFVNAECHPQTIAVVRTRAEPLGWSIKVGDPMKDLEPDAVFGALLQ
                     YPGTSGEIRDFRTVIAALHAAGALAVMASDPLALTLLAPPGELGADIAIGSTQRFGMP
                     MGYGGPHAAFIATKDAYKRALPGRIVGVSVDSRGQPAYRLALQTREQHIRREKATSNI
                     CTAQVLPAVIAAMYAVYHGPDGLRHIAHKIHRTTGTLVKGLAVLGWHVQQTAFFDTVT
                     VEVGARQSEILKNAGSNAINLRKIGDGAQARIGISCDETTTPTVVEAVWRAFGSQAKA
                     QVAAPSFEKLSSQTDNAIPTQLTRRSNFLTHPVFHLYRSETEMLRYMRRLSDRDLALD
                     RSMIPLGSCTMKLNATAEMIPITWPEFANIHPFVPADQAQGYAELIADLSGKLCEITG
                     YDAISLQPNSGAQGEYAGLLVIRAYHVSRGEPQRTVCLIPSSAHGTNPASAQMAGMSV
                     VVVNCDSDGNVDLADLRKKAEQYSSHLAAVMVTYPSTHGVFESAIREICSVVHAHGGQ
                     VYLDGANLNAQVGLARPGGYGADIGHLNLHKTFCIPHGGGGPGMGPIGVKKHLTPFLP
                     GHSEWPSERTQVGAISAATFSSASILPISWAYIALMGGNGLLAATQAAILNANYVAKR
                     LEGHYALLYKGPRGRVAHECIIDTRPFKDTAGVSVEDIAKRLIDYGFHAPTMSFPVAG
                     TLMIEPTESESKAELDRFCDAMISIRSEIAAIEKKAMDRENNVLKNAPHTVFDLTDEN
                     WDRPYARALACFPVKGLLRDKYWCPVNRVDNTYGDRHLVCSCPPMESYQSAAE"
     gene            complement(4760..5647)
                     /locus_tag="VM910_20120"
     CDS             complement(4760..5647)
                     /locus_tag="VM910_20120"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64867.1"
                     /translation="MPIKLAENFRAVFYAPFYAAQSLGFYASEGVEVELVNSSNPGDG
                     VSALLNDAIDITWGGPMRVMKAHDLKPSSPLVCFCEVVARDPFYLVGQHHRPEFQLRE
                     LASLRFARVAEVATPWMCLQHDLRERGIDPSRLDRAPDQPMAANFEALCKGELDVAQL
                     FEPYPAMALELGVGHILYAASTRGPTVYTTFVATRSGIERHREAFTGMTRAIRRMQAW
                     LTQHDAEELAEVTASFFPDIAPDILVSSLRRYGQAGIWARSPDMSRQGFTRLGESLLS
                     GGFISRMPTYEDCVDQSLG"
     gene            complement(5708..6691)
                     /locus_tag="VM910_20125"
     CDS             complement(5708..6691)
                     /locus_tag="VM910_20125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64868.1"
                     /translation="MGGLPRFVAVALVASLNCSGPALSQDWPTRPVTMVVPFAAGGPA
                     DTVGRILAPGLSELLGQQVIIENVGGSGGMTGSSRVAKAAPDGYQFVLGNVGTHAANQ
                     TFYKTPLYNAATDFAPVMLIAQTPLVLLARKDLPANDLREFIAYAKANQASMQYGSGG
                     AGSASHLACVLLNAAVGINVTHVPYRGAAPAMQDLIAGRIDYQCPDTPIAIPQIQSKM
                     VKAIAILTRDRSPGLPGQASAHEQGLTNFDASNWFASFFPKGTSAAIVDKLHAATLAT
                     INTPAVKTRMQEIGADLVARERSSPEYLQKFVETEIEKWAGPIKASGVSME"
     gene            6860..8191
                     /locus_tag="VM910_20130"
     CDS             6860..8191
                     /locus_tag="VM910_20130"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI64869.1"
                     /translation="MSTGARIPDWPTLDATARRKLSLTAPERARRLEPRLNAFVSIEG
                     HLPGVDGKLGGLPYAAKDIFRTASHRPTGGLATPTDVAVDGETDLLARLDGASARRVG
                     FTSLPELAYEPSGYNHARGRVRNPWNPDFVAGGSSSGSAAAVASGSVVVALGSDTAGS
                     LRIPAHCCGVTAWKPTHGIVSCTGAMPLAPSLDTIGLLARSACDMRDAVHILIGDGRL
                     PADGSIERVAAIADCFDAAEPAVRRACLQGVDSIEASAARIERRTSLAEIEAIGGHAL
                     VVLQAEAARVHRARIEDPDIPALLRKRLAKGLAIDGATLAASLSARETLAREFTVRVF
                     GAADAIVLPVMAIRVPRAVETDPDADKFSPAALYALSRFTRFVNLLGFPAVAIPVGFD
                     DQGLPVGLQIVGRPHGDRTVLALAQAVQDRSDWHARVPAAIADDVTDTTEL"
     gene            8224..9102
                     /locus_tag="VM910_20135"
     CDS             8224..9102
                     /locus_tag="VM910_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI64870.1"
                     /translation="MPHLTTDDNVKLYYEDSGSGIPVVFVHEFAGDYRAWESQVRYFA
                     RCYRCITYNARGYPPSDVPADGARYSQERARDDIRAVLDALKIEKAHVVGLSMGGFAA
                     LHFGFTYPQRARSLVVAGCGYGAAPNQRQQFAEEAEAAARKFEQTSMAKAAEAYALGP
                     TRVQFQNKDPRGWKEFVDQLTEHSSEGSAHTLRGVQKRRPSLFDLVDKMKTITTPTLI
                     MTGDEDWPCLEPALLMKRTIPTAGLVVMPNAGHTINLEEPALFNQHLANFFQAVDAGA
                     WRHRDPRAMKEAILGR"
     gene            complement(9175..9906)
                     /locus_tag="VM910_20140"
     CDS             complement(9175..9906)
                     /locus_tag="VM910_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64871.1"
                     /translation="MLQAEGLQLAYGEISACRDVSLHVAQGEIVTLIGANGAGKSTTL
                     RAVAGALLPRAGSIHFRGHDITRLPSYERSKLGISLVPEGRRVFPFLTVRENLELGAF
                     KYRKEGVKVRGLIEKVFDMFPRLRERRSQNAGTLSGGEQQMLALGRAIMSEPHLICLD
                     EPSLGLAPMVVQDIFRTIKAINAAGTSVLLVEQNARYALETASRGYVLQTGSIIASGP
                     CAALRNDDRVRQAYLGRSAAERMRL"
     gene            complement(9912..10649)
                     /locus_tag="VM910_20145"
     CDS             complement(9912..10649)
                     /locus_tag="VM910_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64872.1"
                     /translation="MHKRFGGVQAVRDISLNVPHGSVFAIIGPNGAGKSTLLNLMSGL
                     YQPDAGRMIFDGVDLVGLPAHKRVRLGLARTFQKLRLFKQLSVLGNVVAAAHIHHNIP
                     AWQYVVHAAAFGRDHARCRADAIKLLGFVGLEGRASAQAASLAYGEQRMLELARALAT
                     SPRLLMLDEPAAGLNAAEVERLLERIHLLREGGMTIIVVEHNMDLVMKISDRVLVMDY
                     GQHLFEGTPAEVQANPRVIAAYLGAMT"
     gene            complement(10721..>11306)
                     /locus_tag="VM910_20150"
     CDS             complement(10721..>11306)
                     /locus_tag="VM910_20150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64873.1"
                     /translation="PLGIYAIKPPPDIALPGLPVISFSNTANMFYLVAGFAFLFYLLL
                     DQLVRSPIGETLTAIREDEISAASFGINCTAWKVFAFGVGSALAGAAGAFYASFVGTL
                     VPDAFIITESFTILAMVIVGGMGTLIGPVWGAILLTILPELLRGFGDLRLVLYGAALT
                     LAVLFMPGGIVQAGQMLRKRLGRLPAATNVGTRQ"
BASE COUNT         2199 a         3566 c         3519 g         2022 t
ORIGIN      
        1 ccgaaggagc aaccgccccg gaaactctca ggcaaaagga ccgcgatgat tgacgtctct
       61 ggaaagaggc gcgcgttgcg cccgccgacg ggataacgtt ctcaggcaaa cacacagagg
      121 gggcaccgac acagcggcgc agtcgccgca atgaggtgct ccgatgtggc gcgacacttc
      181 tctcattcgt cttcgaccgg cgcggtccgc taaccagcgg aggcgcgcat gagcgaagtg
      241 caccagaagg cgccgctgct acgcacgcca ctgtataagt ttcatgccga acgcggcgcg
      301 cggctggttg ctttcgcggg ctatgaaatg ccggtgcaat atcccaccgg catcctaagc
      361 gagcatctcc atacccgggc ggccgtcggt ctgtttgatg tctcgcacat gggacagatc
      421 gagctgcgcg cgacgtcggg gcgcctggag gatgcctccc gcgcattgga aacgctggcc
      481 ccgattgaca tcatcggcat cgccgagggg cggcaacgtt acgcgctgtt cacaaacgct
      541 gagggcggaa ttctggacga cctaatggtc gcgaatcgcg gcgaccgtct gctgcttgtc
      601 gtcaacgccg cttgcaagac cgccgacttg gcgcacctgc gtcaaaatct tggacaaacc
      661 tgcaagatcg accctctgcc ggatcgcgcg ctgattgcgt tgcagggtcc gctggcagaa
      721 gcggcgctgg cgcgcctcgc gcccgcgatt actacgatgc gtttcatgga cgtgcgcacc
      781 ctgtcgattc tcggcgcaga ctgcgaggtt tcgcgctccg gctataccgg cgaagacgga
      841 ttcgagatca gtatcgcggc cgatcgcgcc gaggccttcg cacgtgtgct gtgcgaggac
      901 cactcggtga tgccgatcgg ccttggcgca cgcgacagct tgcggctgga agccgggctg
      961 tgtctctacg gttccgatct cgatcccgca ataacgccgg tgatggcctc gctcgaatgg
     1021 gccattcaga aagcccgccg cgccggcgga tcgcgcgcgg gcggatttcc cggcgctgac
     1081 attattctgc gccaattgac ggacggcgcg ccgcaccgcc gtgtcggact gaaagtcgag
     1141 gatcgcgtgc cggtgcgcgg cggcgcgaaa ctctacgcgg acgagatttc ggcaagcacg
     1201 atcggcatcg tcacgtccgg cggctttggt ccgagcgtgc aggcgccgat cgccatgggt
     1261 tacatcgacg aaaactctgc cacgaccggc aaacggattg tcggcgaggt acgcgggaag
     1321 cgcattgcgt tgcaagtctg cgatctgcct ttcgtcccgc atcgctacaa acgcgactga
     1381 aattgaaccg cggagatcaa tcgcgatgtt gaaattcacg gaagaccacg aatggcttcg
     1441 tatcgaaggc gatgtcgcca ctgttggaat cacggaccat gcacaggaac agctcggcga
     1501 tttggtcttc gttgaattac cggcgcctgg agcgcgaccg gaacagggag ccggggcggc
     1561 ggtcgtcgaa tccgtcaagg ccgcctcgga cgtttatgcg ccggtgagcg gcgaagtcat
     1621 cgagatcaat caagcgatcg tcgatgagcc ggcgttggtg aatcgtgatc cgatgggcaa
     1681 gggctggttc ttcaagatca agatttccga accgtcccag ctcgagcgcc tcctcgatga
     1741 gaaggcctac caggcgatga ttgcgtaaaa ctttgcaccg caatcaaact caaactggtc
     1801 cgacaaaggt gaagacgatg cctgccagag cctccgccgc cgattttaac ccgggcgatt
     1861 tttcacgtcg tcacatcggg ccgtcacctg cggacatagg ggaaatgctc tcgactatcg
     1921 gagcgtcgga tatcggtgaa ctcatttcac aaacggtgcc cgatgcgatc cgacaagccg
     1981 ccccgctcgc gctcccggca ggaatttcgg aaaccgaagc gctgcagaag ctccgtatca
     2041 tcgccgataa gaataaaatt ttcacgtcgc tgatcggtca ggggtactac ggaacgatcc
     2101 tgccgccggt gatccagcgc aacgtgcttg agaatccggc ttggtacacg gcctatacgc
     2161 cctaccagcc tgaaatcagc caggggcggc tcgaagctct gttgaatttc cagactatga
     2221 tctccgagct ctgcatgctc gacatcgcga atgcctcgct gctcgacgag gcgaccgccg
     2281 cggcggaagc gatggcgatg gcgcgccgcg tggtcaaatc gcgcgcgaac gcgtttttcg
     2341 tcaacgccga gtgccacccg caaacgatcg cggtggtgcg cacgcgcgcc gaaccgctcg
     2401 gttggtccat caaagtcggc gatccgatga aggacctcga accggacgcg gtgttcggcg
     2461 ctctgctgca atatcctgga acctcgggcg aaatccggga tttccgcacg gttatcgcag
     2521 cattgcacgc ggctggcgcg cttgctgtca tggcgagcga cccgcttgcg ctgaccctgc
     2581 tcgctccgcc cggggaattg ggagcggaca tcgcgatcgg atccacccag cgtttcggca
     2641 tgccgatggg ctatggcggt ccgcacgccg cctttatcgc aaccaaggat gcctataagc
     2701 gcgcgcttcc gggccgcatc gtcggcgttt cggtcgattc tcgcggccag ccggcttacc
     2761 ggctcgcgct gcaaacgcgc gagcagcaca tccgccgcga gaaggcgacc tcgaacatct
     2821 gcaccgcgca agttctcccg gcggtgatcg cggcgatgta tgcggtctat cacggcccgg
     2881 acggtctcag acacattgcg cataagatcc atcgcaccac ggggactttg gtcaagggac
     2941 tggcggtgct cggctggcat gtccagcaaa ccgcgttctt cgacaccgtc acagtcgagg
     3001 tgggcgcgcg ccaatccgaa attctcaaaa acgccgggtc gaacgcgatc aacctgcgca
     3061 agattggcga cggcgcgcag gcccgcatcg gcatcagttg cgatgaaacc accacgccga
     3121 ccgtggtcga ggcagtatgg cgagcattcg gatctcaagc caaggcccaa gtcgccgctc
     3181 cctcgttcga aaagttgagt tcgcaaacgg acaacgcgat ccccacgcaa ctcacccgtc
     3241 gcagcaactt cctgactcac cctgtcttcc atctctatcg ctcggaaacg gaaatgctgc
     3301 gctatatgcg gcggctttcc gatcgcgacc tcgctctcga ccgcagcatg attccgcttg
     3361 gttcctgcac aatgaagctt aacgcgaccg ctgaaatgat tcctattacc tggccggaat
     3421 tcgccaacat tcaccccttc gtgccggcgg accaagccca aggctatgcc gagctgatcg
     3481 cggatctgtc cgggaagctt tgcgagatca ccggctatga tgcgatctcg ttgcaaccaa
     3541 attccggcgc acagggcgaa tatgccggct tgctcgtcat tcgcgcctat cacgtcagtc
     3601 gcggcgagcc gcagcgtacc gtctgcctca tcccgtcatc ggcccacggc accaatccgg
     3661 cgtccgcgca aatggctgga atgagcgtcg ttgtcgtcaa ttgcgacagt gacggcaatg
     3721 tcgacctcgc cgatttgcgg aagaaggcgg agcaatattc cagccatctc gccgcggtca
     3781 tggtcactta tccctccaca catggcgttt tcgaatccgc gattcgcgag atttgcagcg
     3841 ttgttcatgc ccacggcgga caggtctatc tcgatggcgc caacctcaac gcgcaagtcg
     3901 gcctggctcg gcccggcgga tatggcgccg atatcggcca tctcaatctc cacaaaacct
     3961 tttgcatccc gcatggcggg ggcggccccg gcatgggccc gatcggcgtg aaaaagcatc
     4021 ttacgccgtt cctgcccggc cactcggaat ggccgagcga acgcactcaa gtaggggcca
     4081 tcagcgccgc gacgtttagc tcggcttcca tcttgccaat ttcgtgggcc tacatcgccc
     4141 tgatgggcgg caatggcctc ctcgccgcaa cgcaagccgc cattttaaac gccaactacg
     4201 ttgccaagcg gctcgagggc cactacgcgc tgctctacaa ggggccgcgt ggccgcgtcg
     4261 cgcatgaatg catcatcgat acgcggccgt tcaaggacac cgccggcgtc tcagtcgaag
     4321 acatcgcaaa gcggttgatc gactacggat tccacgcgcc gaccatgagt tttccggtgg
     4381 ccggcacgct gatgatcgaa cctacggaat cggaatcgaa ggccgagctc gaccgcttct
     4441 gcgacgcaat gatttcaatt cgatcggaga ttgcggcgat cgagaaaaag gcgatggatc
     4501 gcgaaaacaa cgttctcaag aacgcacccc acaccgtttt cgatctcacc gatgaaaact
     4561 gggatcgccc ctatgcgcgc gcgctggcgt gcttcccggt caaagggctg ttgcgcgaca
     4621 agtattggtg ccccgtcaat cgggtagaca acacctacgg cgaccggcat ctcgtctgct
     4681 cgtgcccacc gatggaaagc taccagtcgg ccgcggagtg atatgagccg ccgcccgaga
     4741 agagacatct gcgacggctt cagcccaagc tttgatccac gcaatcttcg tatgtcggca
     4801 tgcgagaaat aaatccgcct gacagcaggc tttcaccaag ccgcgtgaat ccctgcctcg
     4861 acatatcagg actacgagcc caaatccccg cttggccata acgccgcagc gaactcacca
     4921 gaatgtcggg tgcgatgtcg gggaagaacg acgctgtgac ttcggcgagt tcttccgcgt
     4981 cgtgctgggt gagccaggcc tgcatgcgcc gaatagcgcg cgtcatgccc gtaaacgctt
     5041 cgcggtggcg ctcaattccg ctccgcgtgg cgacgaacgt cgtatagacc gtcggtcctc
     5101 gggtgctggc cgcgtaaagg atgtggccca cgcccaactc aagggccatg gctgggtagg
     5161 gttcgaaaag ctgcgccaca tcgagctccc ccttgcacag cgcttcgaag ttggcggcca
     5221 taggctgatc gggcgcgcga tcgaggcgag atgggtcgat gcctcgctca cgcaggtcat
     5281 gctgcaggca catccaaggc gtggctactt cggcaaccct cgcaaatcgc agtgacgcca
     5341 actctcgaag ctgaaactcg ggacgatgat gctggcccac cagatagaac gggtcgcgtg
     5401 cgacgacctc gcaaaagcag acgagaggcg agcttggctt aaggtcgtgg gccttcatga
     5461 cccgcatcgg cccaccccag gtaatgtcaa tcgcgtcgtt gaggagagcc gatacgccgt
     5521 cacctggatt ggacgagttg accaattcga cctcgacacc ctcgctggca taaaacccga
     5581 gcgactgggc ggcgtagaac ggtgcgtaaa acacggcgcg gaagttttca gcgagcttga
     5641 ttggcatggc gaggatcgat caacgtttga aggcaggtcg gttgtcgcga tcgacggtgt
     5701 cggtcgctta ctccatcgag acgccgctgg ccttgatcgg cccggcccat ttttcaattt
     5761 cggtctcgac gaatttttgc agatattcag gcgagctgcg ctcgcgcgcg acgagatcgg
     5821 cgccgatctc ctgcatccga gtcttcacgg cgggggtgtt gatggtcgcg agcgtggcgg
     5881 catgaagctt gtcgacgatt gccgcggacg ttcccttggg gaagaaggag gcaaaccagt
     5941 tcgaggcgtc gaaattggtc aacccttgct cgtgcgcgga cgcctggcca ggtagccccg
     6001 gcgagcgatc gcgcgtgagg atagcgatcg ccttgaccat cttgctctga atctggggaa
     6061 tcgcgatcgg cgtgtcgggg cattggtaat cgatccggcc ggcgatcaag tcctgcatcg
     6121 ccggggcggc gccgcgatag ggcacgtggg tgacattgat cccgacggcc gcattcaaca
     6181 gcacacaagc aagatggctc gcggaacccg ctccgcccga gccgtactgc atgcttgctt
     6241 ggtttgcctt cgcgtaggcg atgaattccc gcaggtcgtt ggccggcaga tccttgcgcg
     6301 cgagcaggac cagcggcgtc tgcgcgatca acatgaccgg cgcgaaatca gtcgcggcat
     6361 tgtagagcgg agttttatag aacgtctggt tggcggcatg ggtgccgaca ttgccgagta
     6421 cgaactgata gccgtcgggc gccgccttcg ccacgcggga gctgccggtc atgccgccag
     6481 agccgccgac attttcgata atgacctgct ggcccaaaag ctcggaaagg ccgggggcga
     6541 ggatgcgtcc gaccgtgtct gccggaccgc cggcggcgaa cggaacgacc atggtcaccg
     6601 gccgggtcgg ccagtcctgc gatagcgccg gcccgctgca gttgagactg gcgacgagtg
     6661 caacagccac gaaccggggt agccctccca ttcaatcctc cgagccaaaa tggatgaaca
     6721 gaataacagt tgctcggggg ttggatagtc cgcggagcaa tccggtgact caatcgaaaa
     6781 atggagatgt gttagcccgt ggacctcggc ccgcccggcg gctcgcgacc gcaccgactt
     6841 cccggtaaat tcgaacgtga tgtcaactgg cgcccgcatt ccggattggc cgacgctcga
     6901 cgcaactgcg cgtcgcaaac tgagcttgac tgcgccggag cgcgcgcggc ggctcgaacc
     6961 acgcctcaac gcgtttgtga gcatcgaggg acaccttcca ggcgtcgacg gcaagctcgg
     7021 cgggctcccc tatgccgcca aagatatatt tcgaacagcg tcgcatcggc cgaccggagg
     7081 ccttgccacc ccgacggacg ttgccgtcga cggtgagacc gatctgttgg cgcggctcga
     7141 tggcgccagc gcgcgccgcg tcggattcac ctccctgccc gaacttgcct atgagccgtc
     7201 gggttacaac catgcgcgcg gtcgcgtgcg caatccgtgg aaccccgact tcgtggctgg
     7261 cggatcttcg tcgggctcgg cggcagccgt tgcgagcggc agcgtcgtcg tcgcgctcgg
     7321 ctccgacacg gccggctcgc tgcgcattcc cgcccattgc tgcggcgtaa ccgcctggaa
     7381 gccgacccat ggcatcgttt cctgtaccgg cgcgatgccg cttgcgccga gcctcgacac
     7441 catcggcttg ctggcgcgca gcgcttgcga catgcgagac gccgtccata tcctcatcgg
     7501 ggacggtcgg ctgcctgccg acggatcgat cgagcgcgtc gctgcgattg ccgattgctt
     7561 cgacgccgcc gagccggcgg ttcgccgcgc ctgcctgcaa ggtgtcgatt cgatcgaagc
     7621 aagcgccgcg cgtatcgaac ggcgcacgtc gctcgccgaa atcgaagcaa tcggcggcca
     7681 tgccctcgtc gtgttacaag cggaagcagc gcgcgttcat cgcgcgcgca tcgaggaccc
     7741 cgatattccc gcgctcctgc gcaagcgtct ggcgaaaggg cttgccatcg acggtgcgac
     7801 gcttgccgcc agtctatcag cgcgcgagac gctggcgcgc gaattcacgg tgcgcgtgtt
     7861 cggcgcggcc gacgccatcg tccttccagt catggcaatc cgcgtacccc gcgcagtcga
     7921 gaccgatccg gatgcggaca aattctcacc cgccgcgctc tacgcgctga gccgcttcac
     7981 gcggttcgtg aacctgctgg gctttcccgc ggtcgcgatc cccgtgggtt tcgatgacca
     8041 aggacttccg gtcgggcttc agatcgtggg ccgaccgcat ggcgatcgga cagtgctcgc
     8101 gctcgcgcaa gctgttcagg ataggagcga ttggcacgca cgcgtcccag ctgcgatcgc
     8161 cgacgacgtg accgatacga cagagctttg aacgcgacgg agccttgagc aaggggggca
     8221 tcgatgccgc atctgaccac cgacgacaac gtgaagctct attacgagga tagcggatca
     8281 ggcattccgg tcgttttcgt gcatgaattt gccggcgact atcgcgcgtg ggaatcgcag
     8341 gttcgctatt ttgcccgctg ctatcggtgc atcacctaca acgcgcgggg ttatccgcct
     8401 tcggacgtgc cggcggatgg agcgcgttat tcgcaagaac gcgcgcgcga cgacattcgc
     8461 gccgtgctcg acgcgctgaa aatcgagaaa gctcacgtcg tcgggctgtc catgggcggc
     8521 ttcgcggcgc tccatttcgg cttcacctat ccacaacgcg ctcgctcgct ggtcgtggct
     8581 ggctgcggct atggagccgc gcccaaccag cgtcaacaat tcgcagagga ggcggaggcg
     8641 gccgcaagga agttcgagca gaccagcatg gcgaaggcgg ccgaggccta tgcgctcgga
     8701 ccaacgcgcg tgcagttcca aaataaagac ccgcgcggct ggaaggaatt cgtcgaccag
     8761 ctcaccgagc actcgagcga aggttcggcg cacaccttgc gcggcgtcca gaaacggcga
     8821 ccgtccctgt tcgatctcgt tgacaagatg aagacgatca cgaccccaac cctcatcatg
     8881 accggtgacg aggattggcc gtgcctcgaa cccgccctgc tcatgaagcg gacgatcccg
     8941 acggcggggc tcgtggtgat gcccaatgcc ggccacacca tcaatctcga agagccggcg
     9001 ctgttcaacc agcacctcgc caattttttc caggcggtcg atgccggcgc gtggcgccat
     9061 cgcgatccgc gagcaatgaa agaagcaata ttggggcgat gaaggttgat ggatcgattg
     9121 ccgtatatcc gtcgctcgtg aaacgcgtga cgtcgagctc gaaggactcg cgcttcaaag
     9181 cctcatccgc tcggccgcgc tgcggccgag ataggcttgg cgcacgcgat catcgttgcg
     9241 cagggccgca caggggccgc ttgcgatgat cgatcccgtc tgcagcacgt aaccgcggct
     9301 cgcagtctca agcgcatagc gcgcgttctg ctcgaccagg aggacgctcg tgccagcagc
     9361 attgatcgcc ttgatcgtgc ggaaaatgtc ctggacgacc atcggcgcga gcccgagcga
     9421 gggctcgtcg agacagatga ggtgcggttc agacatgatc gcgcgcccaa gggcgagcat
     9481 ctgttgctcg ccccccgaca aggtgccggc gttctggctc ctgcgctcgc gcaaccgcgg
     9541 aaacatgtcg aagaccttct cgatgaggcc tcgcaccttc acgccctctt tgcggtattt
     9601 gaaggcgccg agctcgagat tttcgcgcac cgtgaggaac gggaacacgc gccgcccctc
     9661 gggaacgagc gaaatcccga gcttgctgcg ctcatacgat ggcaggcgcg tgatgtcgtg
     9721 cccgcggaaa tggattgagc cggcacgcgg cagaagcgcg ccagcgacgg cgcgcagcgt
     9781 cgtcgatttg ccggcaccat tcgcgccgat cagcgtgaca atctcgccct gcgccacatg
     9841 caagctgaca tcgcggcagg ctgaaatctc gccataggcc agttgaagcc cctcggcctg
     9901 gagcagcgcg gtcacgtcat cgctccaaga taggcggcaa tcactctcgg attggcttgg
     9961 acctcggcag gcgtgccctc gaaaagatgc tgcccgtaat ccatgaccag cacccgatcc
    10021 gagatcttca tcaccagatc catgttgtgc tcgacaacga tgatggtcat gccgccctcg
    10081 cgaagaagat gaatgcgttc aagcaaccgt tccacttccg ccgcgttgag gccggcggcg
    10141 ggctcgtcga gcatgagcag gcgtggcgag gttgcgagtg cgcgcgcgag ctcgagcatg
    10201 cgctgctcgc cataggcgag cgaagccgcc tgcgccgatg cccgtccttc gagcccgacg
    10261 aagccgagaa gcttgatcgc atccgcgcgg cagcgggcat ggtcgcgccc gaacgcggcc
    10321 gcgtggacga cgtattgcca ggccggaatg ttgtgatgga tgtgtgcggc cgcgacgacg
    10381 ttgccgagca ctgatagctg cttaaagagc cgcaatttct ggaatgtgcg cgcaagcccg
    10441 aggcgtaccc gcttgtgcgc cggcaagcct acgaggtcga cgccgtcaaa aatcatccgg
    10501 cccgcatccg gctgatagag cccgctcatc aggttgagca gcgtcgactt gccggctccg
    10561 ttcgggccga tgatcgcgaa cacactgcca tgcgggacgt tgagtgaaat atcgcgaacc
    10621 gcctgcacgc cgccgaaccg tttgtgcaaa ccttctccgc aaaagagagc agccgtggtg
    10681 gcaggacgcg cttgcaccgc cgcagcaggg cccgacgcgg tcattggcgc gtgcccacat
    10741 tggtagcggc cggcaagcgg ccaagacgct tgcgcagcat ctgccctgct tgcacgatgc
    10801 cgcctggcat gaacagcacg gcgagggtga gcgcagcacc gtagaggacg agacgcagat
    10861 cgccgaatcc gcgcaagagt tcgggcagga tcgtgagcag gatcgctccc cagaccgggc
    10921 cgatcagcgt ccccatgccg ccgacgatca ccatcgccag aatggtgaat gactccgtga
    10981 tgataaaggc atccgggacc agcgttccga cgaagctggc atagaaggcg ccggccgcgc
    11041 cggcgagcgc cgagccgacg ccgaaggcga ataccttcca ggccgtgcag ttgatgccga
    11101 acgaagcagc agagatctcg tcctctctga tcgcagtgag ggtctcgcca atgggcgagc
    11161 ggaccagctg gtcgagcaga aggtagaaaa ggaatgcgaa gcccgcgacc agatagaaca
    11221 tgttcgccgt gttgctgaat gagatgacgg gcagtcccgg cagcgcgatg tccggcggcg
    11281 gcttgatggc atagatgccg agtgga
//
LOCUS       DATLJT010000874         6418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3503773,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000874 DATLJT010000000
VERSION     DATLJT010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3503773"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..745)
                     /locus_tag="VM910_20155"
     CDS             complement(11..745)
                     /locus_tag="VM910_20155"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64874.1"
                     /translation="MNELDFSGKQVFVIGGSSGIGNGIAQAFRKKGARVAVCGTRVHA
                     ADYSAEEGSRLGGLDYTRLDVSDPYAVESFNPYFDRLDVLVLAQGAVIYRRGEFEMSG
                     FRKVLEVNLISLMACATRFHAMLRDTRGALIIVSSTAAFHSTMGNPAYNASKTGAVGL
                     TRTLAQAWAEDGIRVNGIAPGLVDTKMTKVTTENPKRLESALARIPLRRLGTPSDMAG
                     AALFLASPLSSYIIGQTIVVDGGLIL"
     gene            936..1838
                     /locus_tag="VM910_20160"
     CDS             936..1838
                     /locus_tag="VM910_20160"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI64875.1"
                     /translation="MAMAIRQLHKHFVGEVSGLDLRKPLTRDEAVEVEAGMDKYAVLV
                     FHDQDITDEQQMAFALNFGERENPRGGNVTKKEDLRLDSGLNDVSNLDKDGKPLAKDH
                     RQHLFNLGNCLWHSDSSFRPIPAKFSLLSARVVNPKGGNTEFADMRAAYDALDDETKA
                     EIDDLICEHSLMYSRGSLGFLDYSDEEKQLFKPVLQRLVRTHPVHRRKSLYLSSHAGV
                     VRGRSVPEGRLLLRDLTEHATQPEFVYVHKWAVHDLVMWDNRQTMHRVRRYDQSQPRD
                     MRRATVAGTQPTVDQDAAAQQAAE"
     gene            complement(1896..2546)
                     /locus_tag="VM910_20165"
     CDS             complement(1896..2546)
                     /locus_tag="VM910_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HVI64876.1"
                     /translation="MDPEGKRTGNARRKAAGVGDNSAGRGYLDGRLLIAMPVMGDPRF
                     ERSVIYLCAHSSEGAMGIMVNRPAGSIDFPELLVQLNIINKADQIKLPENAETMKVMR
                     GGPVDTGRGFVLHSSDFYLDNATLPIDDKICLTATVDILKAIANGKGPKHAILALGYA
                     GWGPGQLENEIQCNGWLHCDADPDLVFGDDVEEKYVRALRKIGIDPGMLSNEAGHA"
     gene            2684..3523
                     /locus_tag="VM910_20170"
     CDS             2684..3523
                     /locus_tag="VM910_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HVI64877.1"
                     /translation="MTARVPMRAALGFAATLALCSLSLAARADDASPWQRDVHSAVRL
                     LAGSRSGAVLLGGIAFQLQSGWKTYWRTPGDSGVPPRFDFSKSDNVEAVTVLWPAPQK
                     FEDGAGGTSLGYHDQVVLPLRIVAKNVDKPVTLRADINYAVCENLCIPVDASAELAFN
                     SVASTEDSALFAALDTVPKPANVGDPNPLTIRDVKREGKAEVLVDVVTPDARAVNLFV
                     EGPTPDWGLPVPKVREDSPPGVKRFSFQLDGVPPGVDPEGAALKLTLVGADRSYEFNI
                     NLE"
     gene            3648..5012
                     /locus_tag="VM910_20175"
     CDS             3648..5012
                     /locus_tag="VM910_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVI64878.1"
                     /translation="MAAIDETTSLPAGPLARLKATFAGGTSEASVTRRLAGTIFVIRA
                     ISAAFAYLSQILLARWMGGSDYGIYVYVWTWVLLLGSLMDFGISASAQKIIPEYRTRG
                     EGALLRGFLAGSRWMTFVVSAVVSVVLAAVVKLLSPWIEAGAIVPLYIGCLTLPAFVV
                     ANTQDGIARSHDWMQLGLMPQFIVRQALIIGFTAGFFVLGFRLGATAAMVASAGAVWI
                     AMIGQMLVLNRRLSRHIEAGPKAYDVRGWLAISLPILLVESFYLLLSYTDVLVLQQFR
                     PSEEVGIYFAVVKTLALVSFIHYAMSATTAHRFAEYNALGDKARLSAYVAHAINWTFW
                     PSLAATMALLAFGKPLLWLFGPQFVLGYDIMFIAAIGLVVRAAIGPVERLLNMLGHQH
                     ICAVAYALAFAMNVALCVALVPRYGGHGAAAATSFSLTFETALLFWIVRRRLDLHVLA
                     FGNT"
     gene            5215..6042
                     /locus_tag="VM910_20180"
     CDS             5215..6042
                     /locus_tag="VM910_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI64879.1"
                     /translation="MIDPLYVASGFGVGLLVGMTGVGGGSLMTPLLILLFGIHPSTAV
                     GTDLLYAAATKAGGSVVHGLARSVHWPAVIRLACGSIPASILTMVVIWQLDLNPASQR
                     SLVNLVLCFALLLTAVSLIFRKSILDRYRTRMERVEESTIVIATVVTGALLGVLVSIS
                     SVGAGAVGVTALLLLYPRLPMASIVGSDIAHAVPLTLVAGAGHWMLGEVDWQLMGVLL
                     MGSLPGIVIGSYSAVRVPQAVLRVTLAVILLVVAGKMALQEINLPSWTVTAASNISH"
     gene            complement(6139..>6418)
                     /locus_tag="VM910_20185"
     CDS             complement(6139..>6418)
                     /locus_tag="VM910_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI64880.1"
                     /translation="VALELATFKVTCNCISPGYVWTPLVEHQIPDTMKARNMTKEQVI
                     KDVLLEAQPTKEFVTVNEVAALALFLCGDEAAQITGANLSIDGGWTAE"
BASE COUNT         1089 a         2070 c         1978 g         1281 t
ORIGIN      
        1 acgcgatagg ctacaggatc agcccgccat cgaccacgat ggtttggccg atgatgtagg
       61 atgacaaagg cgacgccaga aacagcgcgg cgcccgccat gtcgcttggc gtccccaacc
      121 gcctcagcgg aatccttgcc aatgcactct cgagccgctt cgggttttcc gtcgtgacct
      181 tggtcatctt ggtatcgacg aggcccggcg cgataccgtt gacgcggatg ccgtcctccg
      241 cccaggcttg tgccagcgtg cgggtcaatc ctaccgcgcc tgttttcgag gcgttgtagg
      301 cgggattgcc catggtcgaa tggaaggccg cggtcgacga gacgatgatc agcgcacccc
      361 tggtatcgcg cagcatggca tgaaatctgg tggcgcaagc catcaggctg atcaggttga
      421 cttccagcac cttgcggaag ccggacatct cgaactcgcc acgccggtag atcaccgccc
      481 cctgcgccag cacgagcacg tcgaggcgat cgaaatatgg attgaagctt tcgacggcgt
      541 aggggtcgct gacgtcaagc cgcgtatagt caagaccgcc aagacgcgag ccttcctccg
      601 ccgagtagtc ggcggcgtgt acgcgcgtgc cgcataccgc aacgcgtgcg cccttcttgc
      661 gaaaggcttg cgcgatcccg ttcccgatgc cgctcgagcc gccgatcaca aaaacctgct
      721 tgccgctgaa atcgagctcg ttcatctccc gcaccttacg tttctggctt gtttaaccgt
      781 cttaggactg ccgccggcct tgtcaatcac cccttccgca acgcggaaaa tgcagggctg
      841 gctgccggcc gcttgcgttg acatcattcg gtccgggagt agcttcgttg gaaaatcgcg
      901 cggtaaagcc gcccaccgac ttcaggagaa acgcgatggc gatggcgatc cgccagcttc
      961 acaagcattt cgtcggcgag gtgtccggcc tcgatctgcg caaacccctg accagggacg
     1021 aggcggtcga ggtcgaggcc ggcatggaca aatacgccgt gctggtcttc cacgaccagg
     1081 acatcaccga cgagcagcaa atggcattcg cgctgaattt cggcgagcgc gagaacccgc
     1141 gcggcggcaa tgtcaccaag aaggaagacc ttcgcctcga ctcaggcctc aacgacgtct
     1201 ccaaccttga caaggacggc aagccgctgg cgaaggatca tcgccagcat ctgttcaatc
     1261 tcggcaactg cctctggcat tccgacagtt cgttccgccc gatccccgcg aaattttcgc
     1321 tgctgtcggc ccgcgtggtg aacccgaagg gcggcaacac ggagttcgcc gacatgcgcg
     1381 cggcctatga tgcgctcgac gacgagacca aggctgagat cgacgacttg atctgcgagc
     1441 actcgctgat gtattcgcgc ggctcgctcg ggttcctcga ttactccgac gaggaaaagc
     1501 agctcttcaa gccggtgctg cagcgcctgg tgcggaccca tccggtgcat cgccgcaagt
     1561 cgctctatct ctcctcgcac gccggcgtcg tccgcggcag gagcgtgccg gagggacgac
     1621 tgctgctgcg cgatctcacc gagcatgcga cgcagccgga gttcgtctac gtccacaaat
     1681 gggccgtgca tgacctcgtg atgtgggaca accgccagac catgcaccgg gtgcgccgct
     1741 acgatcagtc gcagccgcgc gacatgcggc gggcgacggt cgccggcacc cagccgacgg
     1801 tggatcagga cgcggcagcc caacaggcgg cggagtaacg ccccgctttc gcgggacttg
     1861 acgaaggcat gacgaaagag gacctgtcgc cgcgcttacg cgtgccctgc ctcatttgag
     1921 agcatgccgg gatcgatgcc gattttgcgc agcgcgcgca catacttctc ctcgacatcg
     1981 tcgccgaaca cgagatccgg atcggcgtcg caatgcagcc agccgttgca ctggatctcg
     2041 ttctccagtt ggcccggccc ccagccggca taaccgagcg cgaggatcgc gtgcttcggc
     2101 cccttgccgt tggcgatcgc cttcaggatg tcgacggtcg cggtcaggca gatcttgtcg
     2161 tcgatcggca gcgtggcatt gtcgagatag aaatcgctcg aatgcagcac gaagccgcgg
     2221 ccggtatcga ccgggccgcc gcgcatcacc ttcatggttt cggcgttttc cggcaactta
     2281 atctggtcag ctttattgat gatgttgagc tgcaccagca gctcgggaaa atcgatgctg
     2341 cctgccggac ggttcaccat gatgcccatg gcgccttcgg aggagtgagc acagagatag
     2401 atgaccgagc gctcgaaccg gggatcgccc atcacgggca tcgcgatcag gagacgacca
     2461 tcgaggtagc cgcgacccgc agaattatcg ccgacgccgg cagctttgcg gcgcgcgttg
     2521 cccgttcttt tgccttctgg gtccatccgc aaggcgctct cgtctatcgc tgcatcctga
     2581 tattgggtgg gtcgtctgtc aatcaagctt ccatcgggcg cgatcgggtc gcatcacaag
     2641 caattcaatg gttcgcaccg gccccagcct gtagagacgt ttcatgaccg ccagagttcc
     2701 gatgcgggcc gctctcggct tcgccgccac ccttgccttg tgctcgttgt cgcttgccgc
     2761 ccgcgctgac gacgcctcgc cctggcagcg ggatgtccat tccgcagtgc ggctgctcgc
     2821 gggatcgcgc agcggcgcag tgctgctcgg cggcatcgcg ttccagctcc agtcgggatg
     2881 gaagacctat tggcgtacgc ccggcgattc cggcgtgccg ccgcggttcg acttctcgaa
     2941 atccgacaat gtcgaagcgg tgacagtatt gtggccggcg ccgcaaaaat tcgaagatgg
     3001 ggccggcggc acctcgctcg gctatcacga ccaggtcgtg ctgccgctgc gcatcgtcgc
     3061 caagaatgtc gacaaaccgg tgacgctgcg cgccgacatc aactatgccg tgtgcgaaaa
     3121 cctctgcatc ccggtcgatg ccagcgccga gctcgctttc aacagcgtgg cgagcaccga
     3181 ggacagcgcc ttgttcgcgg cgctcgatac ggttccaaag ccggccaatg tcggcgatcc
     3241 caatccgctg accatccgcg acgtcaaacg cgagggaaag gcggaggtgc tggtcgacgt
     3301 ggtcacgccg gacgcacggg ccgtcaacct gttcgtcgaa ggaccgacac cggattgggg
     3361 cctgccggtt ccaaaagtca gggaagacag tccgcccggg gtgaagcgat tttcgttcca
     3421 gctcgacggc gtcccgcccg gcgtcgatcc cgagggcgcc gcgctgaaat tgaccctggt
     3481 cggcgccgat cgctcctacg aattcaatat taatctggag tgatctccac gatcccggtt
     3541 ttaccttctc gcacaagggg agagggtaaa gaggtacacc caacccaatc ttaacgaatg
     3601 gttgatagac ggagccgtcg ccgcacactc gcgtgaggat ttccgccttg gccgccatcg
     3661 acgagacgac aagcctgccg gccggcccgc tcgcgcggct aaaggcgacg tttgccggcg
     3721 gtacgagcga agcctccgtc acgcgccggc ttgcaggtac gattttcgtc atccgcgcaa
     3781 tcagcgcggc attcgcctac ctgtcgcaga tcctgctcgc gcgctggatg ggcggatcgg
     3841 actacggcat ttatgtctat gtctggacct gggtgctgct gctcggcagc ctgatggatt
     3901 tcggcatttc agcctccgcg cagaagatca ttccggaata ccgcacgcgc ggcgaaggcg
     3961 cgctgctgcg cggctttctt gccggcagcc gctggatgac ctttgtcgtg tcggctgtgg
     4021 tctccgtcgt gctcgcggct gtcgtcaaat tgctgtcgcc ctggatcgag gccggcgcga
     4081 tcgtcccgct ctatatcggc tgtctcacgc tgccggcctt cgtggtcgcc aacacccagg
     4141 acggcatcgc ccgttcgcac gactggatgc agctcggcct gatgccgcaa ttcatcgtgc
     4201 gccaggcgct gatcatcggc ttcacggccg gtttcttcgt gctcggcttt cggcttggtg
     4261 cgactgccgc gatggtcgcg agcgcgggcg cggtgtggat cgccatgatc ggccagatgc
     4321 tcgtgctcaa tcgcaggctt tcccgccata tcgaggcggg accgaaggcc tatgacgtcc
     4381 gcggctggct tgcgatctcg ctgcccatcc ttctggtcga gagcttttat ctgttactgt
     4441 cctacaccga cgtgctggtc ctgcagcaat tccgcccttc cgaggaggtc ggcatctatt
     4501 tcgccgtggt gaagaccctg gcgctggtat cgttcatcca ttacgcgatg tcggcgacca
     4561 cggcgcatcg cttcgccgaa tacaatgcgc tcggcgacaa ggcgcgcctg tcggcctatg
     4621 tcgcccatgc gatcaactgg accttctggc cgtcgcttgc cgccaccatg gcgctccttg
     4681 ccttcggcaa gccgctgctc tggctgttcg ggccacaatt cgtgctcggc tacgacatca
     4741 tgttcatcgc ggcgatcggg cttgtggtgc gcgcggcgat cggcccggtg gagcggctgc
     4801 tcaacatgct cggccaccaa cacatttgcg ccgtagccta cgcgctcgcg ttcgcgatga
     4861 acgttgcgct gtgcgtcgcg ctggtgcccc gctacggcgg ccacggcgct gcagcggcga
     4921 cctcgttttc gctcaccttc gagacggcgt tgctgttctg gatcgtccgc cgccggctgg
     4981 atctgcacgt gctggcattc ggcaacacat agccttcctg ttttcaccca agagaagcgc
     5041 gtgaccggcg tattggaagc gcagaatacc gctttcgcca ccagcctgcg ataagggcaa
     5101 aaccactctt cgacttcctt atgcggatga attattctta tttggttcga tcggttgaga
     5161 tcaggaaccc tcggccatta gatagccgca agcgtaatcc aagatcgtgc agacatgatc
     5221 gacccgctct atgttgcttc gggattcggc gtaggcctgc tggtcgggat gaccggcgtc
     5281 ggcggcggct cgctgatgac gccgcttttg attctcctgt tcggcattca cccgtcgact
     5341 gccgtcggca ccgacttgct ttacgccgcg gcaaccaagg ccggcggcag cgtggtccac
     5401 ggtctggcgc gcagcgttca ctggccggcc gtgatccggc ttgcctgcgg cagcatcccc
     5461 gccagcatcc tgaccatggt ggtgatctgg cagcttgatc tcaacccggc ctcccagcgc
     5521 agcctggtca atctggtgct gtgcttcgcg ctgctgctca cagcagtgtc gctgatcttc
     5581 cgcaagtcga tcctggatcg ctatcggacg cgcatggagc gcgtcgaaga atcgacgatc
     5641 gtgatcgcca cggtggtgac cggcgcactg ctcggcgttc tcgtttcgat ttcctcggtc
     5701 ggagccggtg ccgtcggcgt gaccgcgctg ctgctgcttt atccgcgctt gccgatggcg
     5761 agcattgtcg gctccgacat cgcgcacgcc gtgccgctca cgctggtggc cggggcggga
     5821 cactggatgc tcggtgaagt cgattggcaa ttgatgggcg tgctgctgat gggctccctg
     5881 cccggcatcg tgatcggcag ctacagcgcg gtccgcgtgc cgcaggcggt gctgcgcgtt
     5941 accctcgcgg tcatcctgct cgtggtcgcc ggcaagatgg cgttgcagga aatcaatctg
     6001 ccgtcgtgga ccgtcacggc ggccagcaac atttcccact gaatgcacga tggcgcgccg
     6061 agcttgagag cgcgataggt cgaatcgcca tcgcgctttg gttcaggcag cgccatcgcc
     6121 gggcatgacg ggtggtacct actcggcggt ccagccgccg tcgatcgaga ggttggcgcc
     6181 ggtgatctgt gcggcctcgt cgccgcacag gaacagcgcc agtgcggcga cctcattcac
     6241 cgtgacgaac tctttcgtcg gctgcgcctc cagcaataca tccttgatga cctgctcctt
     6301 ggtcatgttg cgcgccttca tggtgtcggg aatctggtgc tcgaccagcg gcgtccagac
     6361 gtaaccggga ctgatgcaat tgcaggtgac cttgaaggtc gcaagctcga gcgccacc
//
LOCUS       DATLJT010000875         7174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2585239,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000875 DATLJT010000000
VERSION     DATLJT010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585239"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VM910_20190"
     CDS             <1..607
                     /locus_tag="VM910_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HVI64881.1"
                     /translation="PNQKKTVAALLAGKAPNPAWGEEAKQRSVHNNYLTLPVVFLMIA
                     NHYPLMFATRFNWLIVAIVLAIGPVIRHFFNSRHEGKGSPWWTWGVAAAGMIAVAWLS
                     GAGPADIGPRADRLDIKAAHNAILARCSMCHRNEPVWPGVHAPPKGVILDDPHNIRRH
                     AHLIEINAVRSSAMPPGNVTAMTAQERQILAAWLAAGAPSQ"
     gene            1528..2295
                     /locus_tag="VM910_20195"
                     /pseudo
     CDS             1528..2295
                     /locus_tag="VM910_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
     gene            2398..3225
                     /locus_tag="VM910_20200"
     CDS             2398..3225
                     /locus_tag="VM910_20200"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HVI64882.1"
                     /translation="MIDGDSFAHRAYHALPKTILRRGGQPAGAILGFANFLLKFYRTE
                     QPRAVLVGWDTLDAPTYRHEQFPAYQSGREFDDELLEQLDALPEFVAACGFANAKASG
                     YEADDFLASAAAAEERRGGTVLVASGDRDTFQLASPSTTILYPVRAGEIARIGPAEVR
                     LRYGVDPHQVPDFIALRGDPSDKLPGVAGLGAAGAAQVLHTYGTLENALRAGRFAAQA
                     KSLRLFRSIATMDRKARLPRLSEQKPTWRTAAALARKWELKQLAKRLEELAAQSSTA"
     gene            3743..3916
                     /locus_tag="VM910_20205"
     CDS             3743..3916
                     /locus_tag="VM910_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64883.1"
                     /translation="MGPRATRFALVFWMALAVAVIILAIKPWAGARDSQSKPAAPIPA
                     REIIYQPQHWKVF"
     gene            complement(4280..5416)
                     /locus_tag="VM910_20210"
     CDS             complement(4280..5416)
                     /locus_tag="VM910_20210"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI64884.1"
                     /translation="MNAMTPIELDSSALRSTVAFTGRLACMTRAEAFEVVRSHGGTPS
                     QTVTRQTSVLVVGELGWPLLDDGRPSNKLSKAGSYGIPVVSERHFLEWIGKAVPDSLN
                     RTYSAGQIATLSKLSHGTVEELVRFGLLNPRGGLFGFRDLASARQVSNLFTHGIGLSE
                     IIRSVKEVRAWLPEADLSNLRLHPAAHHSIEIEQPGGRTDKRGQFVLPVSPPAPNADA
                     LFEQAQAAEDAGDIAEAERLYRVLMKCDPMDAAPPFNLGNMLRATGRKVEAEAALRAA
                     TRADPMFAEAWYNLSDLLDEQGRSQTAVDCLRRALEAAPDYVDAMFNLALLLQRNNKH
                     AEAVEYWRRYLANDAQSEWAARARRSLKFCEMQLQHCLSPRSSK"
     gene            complement(5444..6283)
                     /locus_tag="VM910_20215"
     CDS             complement(5444..6283)
                     /locus_tag="VM910_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVI64885.1"
                     /translation="MAPRANWKGYLRLSLVSCPIALYPASSLSEKVSFNRINRKTGNR
                     LKQQNVDSETGEVVPRDDMVRGYEVAKGQYMIVEDEEIEAVQIESTRTIDIDQFVPKD
                     EIDERYIDSPYYIAPDGPVGQEAFAVIRDTIAKLNMVALGRVVLARREHVIALEPKGR
                     GLVGTTLRYPYEVRDEKPYFEDIPELKLPKEMLDLAAHIVQTKSGHFDPSQFEDRYEN
                     ALIDLLKKKQAGEKIEAPRETPPPRVVNLMEALRASVDAEEKKAPAPSTRARRPAKKK
                     ASR"
     gene            6391..6708
                     /locus_tag="VM910_20220"
     CDS             6391..6708
                     /locus_tag="VM910_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64886.1"
                     /translation="MTRRKREITGLMNERDFPHLVELALPPRGFRSVFLEFDAFHRER
                     RIPVRRGRSRQEAEQSYIRFCFPDAAAADAFRNRFGGERLTYAPEKPKPANAALYSRN
                     SKR"
     gene            complement(6919..>7174)
                     /locus_tag="VM910_20225"
     CDS             complement(6919..>7174)
                     /locus_tag="VM910_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI64887.1"
                     /translation="SPLYADPTGLPPTLIQVGSDEILLDDSVRMAERMRAAGCQVELE
                     IWPRMPHTWQVWARVLPEARAAIERIGAFVQSKVEPQAVR"
BASE COUNT         1387 a         2258 c         2115 g         1414 t
ORIGIN      
        1 cccgaaccag aagaagaccg tcgcggcgct gctcgccggc aaagcgccca atccggcgtg
       61 gggcgaagag gccaagcagc gctccgtcca taacaactac ctcacgctgc ccgtcgtctt
      121 cctgatgatc gccaaccatt atccgctgat gttcgcgacc cgtttcaact ggctgatcgt
      181 cgcgatcgtg ctggccatcg gtcccgtcat ccgccacttc ttcaattccc ggcacgaggg
      241 caaaggcagc ccgtggtgga cgtggggcgt ggcggccgcc gggatgatag ccgtggcatg
      301 gctgtcgggc gcgggacccg cggacatcgg tccacgcgca gatcggctcg acatcaaggc
      361 ggcacacaat gcgatcctcg ctcgctgcag catgtgtcat cgcaacgaac cggtgtggcc
      421 tggcgtgcat gcgccgccca aaggagtcat cctcgacgat ccccacaaca tccgccgtca
      481 cgcgcatctg atcgagatca acgcggtgcg ctcgagtgcc atgccgccgg gcaacgtcac
      541 cgcgatgacg gcgcaagagc gccaaatcct cgccgcctgg ctggcggccg gcgcgccgtc
      601 gcagtgacga atgtcgcttc tgcattgcag cgattacggc taaccccgtc gccgggacct
      661 cataaggaca gcatcaggtc cgtcgggcgt ggccaacggt tatcgccaac gttccgcgtg
      721 gggaactctc ggaacatcct tgcccgtgtc gcgtttgccc aaggctacaa ctccaaggct
      781 acaactatgg aggccagagg atgcgaacag ctcatggtat tgctatcgcg atcgcgatag
      841 cgggttctgc cgcgctctcg ttgccagcga gcgcggaccg gatctgcagg caagactgcg
      901 tcgggccgct gtgcaagaaa gaatgcgtcc accgcgaccg cgacatcacg gtcggcaggg
      961 gcgtccgcga ccgcgatgta acaatcgagg agcgccgccg cgtgcgagag ccgggtgttg
     1021 agatcagaag gtctcgtccc ggcgtcgatg tcgacattcg gtgacccgtt ggccccgccc
     1081 gattgggcgg ggcctttcct ttcagccggc tcgaccatca ctgaattggt tggaaccaaa
     1141 gcccggcctt gcggttatag aacctgagtg cccagaggag cgatgccgga tgtacgcacg
     1201 ctctcaggcg ctttctggtt ttattttgca aaaatcactt cggtaatcgg cagcgccgct
     1261 catagccacc gccgctcatc acgacaaaat ctcgagcgcc tgcgctttcg ctttcgacgt
     1321 tggtcgagcg ccaaacggcc gacacgcgcc ctgccgggtc gtgcgtctgc ctccgcgccg
     1381 cgcatttgtc gccccgggcg ggggccatca attctcgcag ccacgatctt ccatagtttt
     1441 gatcgtcaac gcccccatgg gctcgtcggt tcggctacgt taggccgcct ccgtcaaatg
     1501 catttagcaa taaccgtgcg gggcgggatg aaaatcgcga ccttcaacat caacaatatc
     1561 aacaaacgcc tcgccaacct cctgcattgg ctgcgcaccg ccaagcccga cgtcgtctcg
     1621 cttcaagaac tcaaggcggc ggatgcggag tttccgaagg cggcgctggc gaaagtcggt
     1681 tatggcgcgg cttggcgcgg ccaaaagtcg tggaacggcg tcgccatcct cggccgcgat
     1741 tgcgagccca tcgtcacgcg cacggagctg ccgggcgatc cagctgacac ccagagccgc
     1801 tatatcgagg cggcggtgca tggcgtgctg atcacgtcgc tctatgcccc gaacggcaac
     1861 ccacagccgg gccccaaatt cgcgtataag ctcgcttgga tggagcgcct tgcgacccat
     1921 gccgaggagc tttacggcgc agggatcccg gtcgtgctcg cgggcgacta caacgtcgtg
     1981 ccgacggacc gtgatatcta tccaacgcaa tcctatgcga aagacgcgct cgttcagccg
     2041 gaaagccgcg ccctttttca gcgcattctc gaccaaggct gggttgacgc aatccgcgcg
     2101 ctgcatcccg atgcgccgat gtatacgttc tgggactaca tgcgaaaccg atggcctcgg
     2161 gacgcgggcc ttcgtatcga tcatttgctg ctgagcgccc gagccgtgga gcgccttgtc
     2221 agcgcgggcg tcgatcgtca aattcgaagc agacaaggcg ccagcgatca cgccccggct
     2281 tgggtcgtgc tgcgcaacga tgcgaagccg cggcgaaatt ctccgagccc caagcgcaag
     2341 accgcgcgag gttcgctgcg gcggaacgca ccagcgccga ggcgtcggcc actgctggtg
     2401 atcgacggag attccttcgc acatcgtgcc taccatgctt tacccaagac cattttgaga
     2461 cgcggaggcc agcctgccgg tgcgatcctt ggcttcgcga atttcctgct gaagttctac
     2521 cggacggagc aaccgcgcgc ggtgctggta ggttgggaca cgttggacgc gccgacctac
     2581 cgtcacgagc aattccccgc ctatcagagc ggtcgcgagt tcgacgacga attgctcgag
     2641 caacttgacg cgttgcccga attcgtcgcc gcctgcggct ttgcgaatgc caaggcatcc
     2701 gggtatgagg ccgacgactt tcttgcctcc gcggccgcgg ccgaagagcg gcggggcggt
     2761 acggtcctgg tcgcaagcgg cgaccgcgat acattccagc ttgcctcccc cagcacgacg
     2821 attctttatc cggttcgcgc tggcgagatc gcccggatcg gtccggccga ggtccgcttg
     2881 cgctacggag tcgatcccca ccaggttccg gatttcatcg cgttgcgcgg cgacccatcg
     2941 gacaagctgc cgggagttgc gggactgggg gcggcgggag cagcacaggt gttgcacaca
     3001 tacggcacgt tggagaatgc ccttagagcg ggtcgctttg cggcgcaggc caagagtttg
     3061 cggctgttcc gatcgatcgc caccatggac cgcaaggcac gcttgcctcg tctgagcgag
     3121 caaaagccga cgtggcgcac agcggccgcg ctggcgcgga aatgggagct caagcaattg
     3181 gccaagcggc tcgaggagct ggcggcgcaa agcagcactg cctgatcctg agatgattag
     3241 ccacacagcg ataggatcaa cgagtggcgc gccatgctgg atgtttctgg cggcctgccg
     3301 ttctcgaggc tcgcttaagg gcgaatccag ttttgggaac cgacaaccac gaaatcagcc
     3361 atagcggtgc gagctgcgcg gtaatagcgc atttttgtga ttgccatcac atcgcgagcg
     3421 catttttgtg atcgccatca catcacgaac gccatggccg agctagcctt gcgattgtcg
     3481 atcggtaccg catatgcggt tctcacgttc gacgttcctc cgaaacactg agccccgctc
     3541 acgcggggct cctttttttt ccaggccgga aacagccaga cttattccat tacctcgttg
     3601 gcgcgcacga gccgcacgcg atcgccgcca ggggaatcag caccgctctc cgagtgtaac
     3661 gcagctcaca aagcccttcc ggtttccgct ttaaagtccg ccgcgacgca caacgatgtc
     3721 aatttggcat cgggaggagg atatgggacc cagggcaacg aggttcgcgc tcgtcttctg
     3781 gatggccctt gcggtggcgg tgatcatcct ggcgatcaaa ccgtgggcgg gcgcgcgaga
     3841 ctcgcagagc aagccagcag cgcccattcc ggcgcgggag atcatctatc agccgcaaca
     3901 ctggaaagtg ttttgaagac cggctgatcg tccgcagaaa cccaagcctt cccgacaccg
     3961 gcataggatc gatcggggcg gacacgcgcg actctaagcc tccgcgcgca agacacgctt
     4021 gagccgaagc tgtatatgat agcgtcactt gccgttttgc tttgtctcat cccgtgctga
     4081 aatgtccgat ttcttgtgcc tttcgtacac gctgccgcgc tcaccatacc aacaaatgtt
     4141 ggccagtgct gaactgttcg gtgccaattt gggtgccaat tcttacacgc tgtaattggt
     4201 cacccgctat ctcctgccga tagctcccgc tgatcgcacc ggcggccacg ccaaggtcgg
     4261 cgcggggcat cacacgcagc tacttggaag accgcggcga taagcagtgt tgcagttgca
     4321 tttcgcagaa ttttagggat cgccgcgctc gtgctgccca ttcagattga gcgtcattgg
     4381 cgagataacg ccgccagtac tctacggcct ccgcgtgctt gttgtttcgc tgaagtagta
     4441 gcgcgagatt aaacatcgcg tcgacatagt cgggcgcggc ctcaagcgca cggcgcagac
     4501 aatcaaccgc ggtttgtgag cgaccttgct catccaacag gtcggaaagg ttgtaccatg
     4561 cctccgcgaa catcggatca gcgcgagtcg ccgcacgcaa cgccgcttcc gcttcaacct
     4621 tgcggccggt agcccgaagc atgttcccaa gattgaacgg tggcgccgca tccataggat
     4681 cgcatttcat caaaacacga tacaaccgct cggcttcggc aatgtcgccg gcgtcctctg
     4741 ctgcttgtgc ttgctcaaat aaagcatcgg cgttcggggc tggcgggctg accggcaaca
     4801 caaactgccc gcgcttgtcg gttcgtccac ccggctgttc gatttctatg gaatggtgag
     4861 cggccgggtg caggcgcaaa ttggacaagt cagcttcggg aagccatgcc cgcacctcct
     4921 ttacactccg aatgatctcg gaaagaccaa tgccatgtgt gaacaggttc gagacctgcc
     4981 gggcacttgc cagatcgcga aacccaaata ggccgcctcg cggattcagt aggccaaagc
     5041 ggacaagctc ttcaacggtg ccgtgtgaaa gcttgctcaa tgtggcgatc tgaccggccg
     5101 agtaggtcct attcaaactg tccgggacag ctttcccgat ccactcgaga aaatggcgct
     5161 cgcttaccac tggaatccca tagctgcctg ctttgctcag cttgtttgat ggtcgcccat
     5221 catccagcag cggccagcct agctcgccaa caacgagtac actggtttgt cgggtaactg
     5281 tttggctggg cgttccgcca tgtgaacgca ccacctcgaa cgcttctgcc cgtgtcatgc
     5341 aggcgagccg gcctgtgaag gctacagtgc ttctcagcgc cgagctgtcc aattcgattg
     5401 gggtcatcgc gttcatagtc gctctccgca ggagcgactc aatttaccga cttgccttct
     5461 tcttagcagg ccggcgagcg cgcgtacttg gcgctggggc cttcttttcc tcagcatcca
     5521 cactggcgcg gagggcttcc attaggttga ccactcgcgg cggaggagtc tcacgaggtg
     5581 cttcaatttt ctcgcccgcc tgtttcttct tcaagagatc aatcaaagcg ttttcgtagc
     5641 gatcctcgaa ctgcgatggg tcaaagtggc cagacttcgt ctgtacgatg tgggcagcca
     5701 gatcgagcat ttccttcggc agtttcagct ccgggatgtc ctcgaaatat ggcttctcat
     5761 cccgaacctc gtaaggataa cgcagcgtcg tacctacaag gccccggccc ttcggctcga
     5821 gcgcgatcac atgctcgcgc cgcgccagca ccacccgacc gagcgcgacc atgttcagct
     5881 ttgcaatggt gtcccgaatg acggcgaagg cttcctgacc caccggccca tccggggcta
     5941 tgtaataggg cgagtcgatg tagcgctcat cgatctcatc ttttggaacg aactggtcga
     6001 tgtcgatggt ccgcgtgctc tcgatctgaa cggcctctat ctcctcatcc tcaacaatca
     6061 tgtactggcc cttggcgacc tcgtagccgc gcaccatgtc gtcccgcggg accacctcgc
     6121 cagtttcgga atcgacgttc tgctgtttca accggttgcc cgttttccgg ttaatccgat
     6181 tgaaactcac tttttcgctc agggaagagg ccggatagag cgcaatgggg caagagacca
     6241 gtgaaagacg aaggtacccc ttccagttcg cgcgcggcgc cattggatac tcctgaacag
     6301 tatgcggcgg gccaagttat accgtaaaat ttagcaaatg cttgaggcag agggtcattt
     6361 catcgtaacc ggccgatcat cgaggtacga atgacccgtc gcaagcgcga gatcaccggc
     6421 cttatgaacg agcgggactt tccgcatctg gttgaactcg cacttccgcc acgaggcttc
     6481 cgcagcgtct tcctagagtt tgacgccttc caccgtgaac ggcgcatccc agtgcgccgt
     6541 ggccggagtc ggcaggaggc agagcaatcc tatattcgat tctgcttccc cgacgctgcc
     6601 gctgcggatg cattccgcaa tcgcttcggt ggcgagcgct tgacctacgc accggagaag
     6661 cccaagccag cgaacgcggc tctttacagc agaaatagca aacggtgaaa caatgcaggc
     6721 cgtgggttga cctttctgag atgtcgccct gatagcgcca gcatctgaga aattgttggc
     6781 ggcgcgggcg agttcgtcaa aacgatccat ggacccccgg acaaaaacgg gccagcggac
     6841 tcaagaccgg acttacgaga gagccggttg ctgtggcacg ggcacctgcg ccgcgccaag
     6901 gccaaaggcg cgaccgttct agcgcaccgc ttgcggctct accttgcttt gcacgaaggc
     6961 gccgatccgc tcgatggccg ctcgcgcttc cggaaggacc cgcgcccaca cctgccaggt
     7021 gtgcggcatc cgcggccata tttcgagttc gacctgacat ccggcggcac gcatccgctc
     7081 cgccatgcgc acggaatcat cgaggaggat ctcgtcgctg ccgacttgga tgagcgtcgg
     7141 cggcagtccg gtcgggtcgg cgtaaagcgg cgac
//
LOCUS       DATLJT010000876         3298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_57445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000876 DATLJT010000000
VERSION     DATLJT010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM910_20230"
     CDS             complement(<1..195)
                     /locus_tag="VM910_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVI64888.1"
                     /translation="MRRVRPVRVLWVGCAALLALAASTMSAGATSRIKDLANIEGVRQ
                     NQLIGYGLVVGLNGTGDTLNN"
     gene            466..882
                     /locus_tag="VM910_20235"
     CDS             466..882
                     /locus_tag="VM910_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HVI64889.1"
                     /translation="MRIYGPNGTTPGAPASSTKRISSTGFSLPEDLNSATETRAAVAP
                     KATANIDALLALQGIEEDPVERRRRSVQRGKGALDVLDDLKLGLLSGNLDSSTVSRLR
                     DAAANLKSSSGDPRLDAVLSEIELRVEVELAKAGQY"
     gene            complement(889..1260)
                     /locus_tag="VM910_20240"
     CDS             complement(889..1260)
                     /locus_tag="VM910_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI64890.1"
                     /translation="MKSANAYSADCPTRQILDRIGDKWAVLILLLLRDEPLRFNQLRR
                     AVEGISQKMLSQVLKSLERDGLVRRRVIPTVPVTVEYSLLPLGATLAAAVDPLREWAE
                     RNLKEVLAAQRRYDTQHKAAA"
     gene            1682..2047
                     /gene="dksA"
                     /locus_tag="VM910_20245"
     CDS             1682..2047
                     /gene="dksA"
                     /locus_tag="VM910_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HVI64891.1"
                     /translation="MNDRQREYFRAKLLAWKDEILRESRLTLQTLQEENVNHPDLADR
                     ASSETDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIAT
                     LSVEAQERHEKREKVYRDE"
     gene            2417..3202
                     /locus_tag="VM910_20250"
     CDS             2417..3202
                     /locus_tag="VM910_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI64892.1"
                     /translation="MDRILAAAQTIATARRSRTALKALPPDVAPRDEAEGYLVQRALH
                     DLLLPQTGALVGYKIGCTSKVMQEYLDIPHPCGGGVFARGVFESGARVGASDYVRAGV
                     ECEIAVRLGRDLAASEEPFTAEWVMEAVEAYFPAIEIVDDRYEQWESLGAPTLIADDF
                     FAAGCVLGEPVARTHAPDIAHVKGRAIVNGMEAGRGTGVDVLGHPHNALAWLANHLAE
                     EGRGLHAGQIVLTGSLVKTVWLKPGAHVTMELEGLGRVEAVFA"
BASE COUNT          615 a         1017 c         1066 g          600 t
ORIGIN      
        1 gttgttcagc gtgtcgccgg tgccgttgag gccgacgacg agcccatagc cgattagctg
       61 attctgccgc acaccttcga tattggcgag atccttgatc cgcgacgtcg cgcccgcgga
      121 catggtcgac gccgccagcg caagcagcgc ggcgcagccg acccaaagga ctctcaccgg
      181 acggacgcgt cgcatcccaa agctcccctc gcactacttc ccgagatcgg ccaggaccac
      241 tcccatagtc ctacgatccg cttctgctat gcgagccccg tgccatgccg caacgccggc
      301 gtaccgttct gattttgttg aataaagctt gttcatgcgg ggaaaggcga cggtgaccgg
      361 gccgggcaaa accggcgcat gccggcagat tttgccgagc cgtttacgtt gcattaacca
      421 taagagggcg aagctcccgc catctcaccg acgcgggatc agacgatgcg catctacgga
      481 ccgaacggga ccacgcctgg cgctcccgca agcagcacca agcggataag ttcgaccggc
      541 ttttcgctgc cggaagatct gaactcggcg acagaaaccc gcgctgccgt cgcgccgaag
      601 gccaccgcca atatcgacgc gctgctcgcc ttgcagggga tcgaggagga tccggtcgag
      661 cggcgccgcc gctctgtgca gcggggcaag ggcgcgctcg acgtgctcga tgatctgaaa
      721 ctcgggttgc tctccggcaa tctcgacagt tcgacggtta gcaggctgcg tgacgccgcc
      781 gcgaacctga aatcctcttc cggcgatccc aggctcgatg cggtgctctc cgaaatcgaa
      841 ttgcgcgtgg aagtcgaact ggccaaggct ggtcagtatt agattgggtc acgccgccgc
      901 cttgtgttgg gtatcgtagc ggcgctgcgc ggccaaaact tccttcaagt tgcgctcggc
      961 ccattcgcgc aagggatcga ccgcggcggc aagcgtcgcg ccgagcggga gaagcgaata
     1021 ttccaccgtc accggaacgg tcgggatgac gcggcggcga accaggccgt cgcgttcgag
     1081 gctcttgagc acctgcgaca gcattttctg cgaaatgcct tcaacggcgc ggcgcaactg
     1141 attgaagcgc aggggctcgt cgcgcaacaa cagcaggatc agcaccgccc atttgtcgcc
     1201 gatgcgatcg aggatctggc gggtcggaca atcggccgaa taggcattgg cggacttcat
     1261 gcggttactc cggcgcagtc gatcgagctg gttttcgtct aacacttaca tcctctacga
     1321 aatctagtca ctattgtata ccgacgggca atttgggcgg gcgggttccg actcgctgct
     1381 ggccgcctcc tgccggccgg cgattcgagc ccacggccca tgtcaggaag cgctttacgg
     1441 tgggctttcg gcggggttgg gctagccccc ctaagggcgg ataaaccagc ctgtgcaagg
     1501 ttttgggggt ggccgctgtt ccgtgccggc tgatattgtc tgtcacaagg ccccgctata
     1561 taaggcgcgc gcaaacgggc ttgccgttcg ccgggtgccg cgggaaaccg gacttgcacg
     1621 tgcagatagg ctgaccttgg aaaaactgaa gaattaccga ccttctgaaa aagagccttt
     1681 tatgaatgat cgccagcgtg aatattttcg cgcaaagttg ctggcgtgga aagatgagat
     1741 cctgcgggag tcgaggctga ccctgcagac cctgcaggaa gagaacgtca atcatcccga
     1801 tcttgccgat cgggcctcct ccgaaaccga ccgcgcgatc gaattgcgtg cccgcgaccg
     1861 ccagcgcaag ctgatctcca agatcgacgc ggcgctgcag cgcatcgagg acaacaccta
     1921 cggctactgc gaggaaaccg gagagccgat ctcgctgaaa cggctcgaag cgcgcccgat
     1981 cgcaacgctg tcggtcgagg cgcaggagcg ccacgagaag cgcgagaagg tctatcggga
     2041 cgagtagcaa agggctctcg atcaattgcg catgatcttc gcgcaagcgc attccgcgtg
     2101 tgtcgcgggg gaaacccgcg tcctgctttt ccggatcagg tgctggttcc ttgccggcct
     2161 cgaatgccgg cagtaacggc cgtgaatttg ccggtctgca tgcagtgatg gtagcgctcg
     2221 acgcatttcc ttgcatcgcc gccgttgcgg atgtcgccgt ggatcgcctg cgagcatgag
     2281 ggcgcgccgc ggtttccgag cccaccacaa ccgccgcctc ccacgaactc gacgatgtct
     2341 ttagtggacc ctcgggacgg tcgaagcgat aaagaatcaa gtgccaacgg cttgccggcg
     2401 gcgctaagat gttcgcatgg acaggatcct cgctgcggcc cagaccatcg cgaccgcgcg
     2461 gcgcagccgg accgccttga aggcgcttcc gcctgacgtt gcgccgcggg atgaggccga
     2521 aggctatctg gtgcagcgcg cgctgcacga ccttctgctg ccgcaaaccg gtgcgctcgt
     2581 cggctacaaa atcggctgca ccagcaaggt gatgcaggag tatctggaca ttccgcatcc
     2641 atgcggcggc ggcgtcttcg cgcgcggcgt tttcgaatcc ggcgcgcgcg ttggcgcctc
     2701 cgactatgtt cgggccggcg tcgaatgcga gatcgcggtt cggctcgggc gtgatcttgc
     2761 ggcctcggaa gagccgttca cggccgagtg ggtcatggag gcggtcgagg cgtattttcc
     2821 ggcgatcgag atcgtcgacg accggtatga gcaatgggaa tcgctgggcg cgccgacgct
     2881 gatcgccgac gacttctttg ccgccggctg cgtgctgggc gaaccggtcg cgcgcacgca
     2941 cgcgccggac atcgctcacg tcaagggccg cgccatcgtc aacggcatgg aggccggacg
     3001 cggcaccggg gtcgacgtgc tcggtcatcc acataacgcg ctcgcctggc ttgccaatca
     3061 tctggcggag gaaggccgcg gccttcatgc cggccagatc gtgctcacgg gaagcctcgt
     3121 caaaaccgtg tggttgaagc cgggcgcgca tgtcacgatg gagttggagg gactcggcag
     3181 agtggaggcg gtgtttgcgt gaggtggaag agggcacgcg aaggatgggc cgtcagatga
     3241 tttttggcac aacctcacat ccccgcggcg ctgttacgcc cgagccgttg tgaatgcc
//
LOCUS       DATLJT010000877         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_744065,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000877 DATLJT010000000
VERSION     DATLJT010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744065"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VM910_20255"
     CDS             <1..214
                     /locus_tag="VM910_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64893.1"
                     /translation="AQHKGISIMKIVIIVTAGLFAMLSVAAAADLPRKEPPPVAAAPI
                     GKYPVGKYPVGKYPVGKYPQPVVTKG"
     gene            606..1193
                     /locus_tag="VM910_20260"
                     /pseudo
     CDS             606..1193
                     /locus_tag="VM910_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
     gene            1755..>3428
                     /locus_tag="VM910_20265"
     CDS             1755..>3428
                     /locus_tag="VM910_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI64894.1"
                     /translation="MWARIRETLADWYILIFAICLIAGGMFAASHQAAAPTTTQQSTT
                     QTPTAPSPANVATSQQSQRPPSATPPAPPQPSAQKTPTTPAGSPAASVPPPDNSAADK
                     QTSPPASHDHGMQTMAANAPPPSSAGGPAPVAAVVPAATGGGDAAAGRLVYRKCQACH
                     SMDAGKNLLGPSLAGIMGRKAGAESGYNYSPAMKQSGLVWDGNTLDAYLADPQKLVPG
                     NKMPFPGLKTDHDRADIIAYLAAGAQPAPAAPNPAGQPASGATAQNTPAPASQPPQGA
                     SGADITYIPDARYTLRSGIAEGRMVYIGVGGTIDGKVNPVLTASEGQVVQVTLINGEG
                     AEHDIVFPDQDAKSPRVTGKGSSTTIAFRATKSGDFIYLCSVPGHQLAGMQGQFLVTP
                     RPTPQTVVEADISREATDLSPPIGKRDPQTVRVDLFRVEIEGRLAEGTTFGYWTFNGK
                     VPGPFIRVRIGDTIDIHLKNSADSSMIHSVDFHATTGPGGGAAALQVDPGKEKSMTWK
                     ALVPGLYVYHCATPMVAEHIANGMYGLILVEPEGGLPPVDREFYVMQGEI"
BASE COUNT          727 a         1074 c         1026 g          601 t
ORIGIN      
        1 tgcccaacac aaggggatta gcatcatgaa gatcgtcatc atcgtaacgg ctgggctctt
       61 cgccatgctg agcgttgccg ctgccgccga cttgccgcgc aaagagccgc ccccggtggc
      121 ggcggcgcca atcggcaagt atcccgtggg caaatatccg gttggcaagt atccggttgg
      181 caaataccca cagcccgtcg tgactaaggg ctaaggcgag ctatcgcgcg ctttgtgaaa
      241 gctcaacgcc gacgcggccc ggttcacgat cgtcccgcgc gccgacctct cgaacctttc
      301 gctcgtggac gaagagtcac aagcgcgggc tttcctttga cgcaaatgaa ccgggcccgg
      361 ggaaatgggg ggcaaacatg cgactcttaa atgggtttgg ctgtttcgta tgcggtagct
      421 tggcagttct gctttgtgct aaccgcgcga atcctgaccg acatgctgat gcagcccttg
      481 ctcgcatgga tgcaatagct gcactcgctc cctcagatgc cgcggccgcc gtcgctgatc
      541 cagtcataaa agtgccgctt gccgaaccag aggttaatgt gagtccgctc gaagcttcgg
      601 atgggtgcgt cctgagcgaa gagtgcatcg accaatatct ctggtcggtg tacgaccgcg
      661 cgcccaaggt cgataccatc aaagtggaga aacagatcga gacgacggtc aagaagaacg
      721 gcaagagccg cactgtcacc aaaactgtta cgaagctcgt gaacgaggac ttcacgtgga
      781 aggatccaga tgccgccgag aaagctggca tgtccgtggc ccaatacgtg atcggcggca
      841 tggaccgggc cttcaaagcg aggctctatc acctctttcg cgcgcttgac gacgccgggc
      901 ttgctcctgg gatgacaagt ggcttccgcg atgactaccg gcaatcgatc gcaagcggca
      961 acaaggccgc aaccaatcgc tcttaccatg gcggaagcct gcgcggcggc tatggccatg
     1021 ggcttgcagc cgatatcgtg agcgtcaagg gtgagacgcg ttccgagcga tgtatttcaa
     1081 gccaaagctt gtggaagtgg atcgacgcgc acggcaagga atatgggatc gggagaccgt
     1141 atctcgataa agatccgccg cacattgcgc cgatcgacgg caaagaatac gccgataagc
     1201 gcggagtgaa tgcgaaactc gcggaagagg gggacgcttc cacgagcgga gtcgcaaaag
     1261 cggctaaaca gggacgggcc gaacctaagc cgctttgatg cttgcggtgc gttagctcgt
     1321 ccggcttgcg cctgtttgac ccgatgcctc gggaaagccg aagtgcagct tgcagcgatc
     1381 gcagggggtg cgcctcgtgg gtcacaacgc attggtcacc gaccacgcgg cgatcgaggc
     1441 ggagattgat tgaaatcccg cggcgttgcg ctcggggcaa agcgcacttg gtctaagcga
     1501 cgtgacgttg gcttatgacc ccaaccggac ctcaggtcgc gtttcagaac gattcagatt
     1561 ttaccccgag atagtcggcg cagattcgga atggagccgc gcatcggctt ttttgcacga
     1621 gttactcgcg cttctgaagg aagcaattcg aaaccttgaa aaccggtcga cgttcctgtc
     1681 agacttcgct tctcgttccg acagatgccg aacgggcgga aacccggtcc tttgaaccgc
     1741 gtggggaggc gacgatgtgg gcgcgaatcc gcgagacgct ggccgactgg tacatcctta
     1801 tctttgcaat ctgcctcatt gctggaggca tgtttgccgc ctcgcatcag gcagcggcgc
     1861 cgacaacgac gcagcagagc acaacgcaga cgcctacagc tccatcgccg gcaaatgttg
     1921 cgacgtcgca gcagtcacaa cggccaccgt ccgcaacacc acccgcgccg ccccagccgt
     1981 cggcccagaa aacgccaacg acaccagccg gttcacccgc agcttccgtt cccccacccg
     2041 ataacagcgc ggcggacaaa cagacttctc cgcctgcgtc gcacgatcat ggcatgcaga
     2101 caatggctgc gaacgcgccg cctccttcga gcgcgggcgg accagctccc gtcgcggcag
     2161 tcgtcccggc tgccactggc ggcggcgacg ctgcggccgg ccgtcttgtg taccgaaaat
     2221 gccaggcatg ccattcaatg gatgccggta agaatctgct gggtccttcg ctcgcgggca
     2281 tcatgggtcg taaggcgggc gccgagtccg gctacaatta ctctccggcg atgaagcaga
     2341 gcggcctcgt ctgggacggc aatactctgg atgcctatct cgccgatccg caaaagcttg
     2401 ttcccggcaa caagatgccg ttccccgggc tcaaaaccga tcacgaccgc gccgacatca
     2461 tcgcttatct ggccgcaggc gcccaaccgg caccggcggc ccccaaccca gccggtcagc
     2521 cggcatcggg cgcaaccgcg cagaacaccc ccgcgcctgc gagccaaccg ccgcaaggcg
     2581 cctccggagc ggacattacc tacatacccg acgcgagata cacattgcgg tcgggaatcg
     2641 ccgaaggccg catggtgtac atcggcgtcg gcggaacgat cgacggcaag gtcaatccgg
     2701 tcctgaccgc atccgagggg caggtcgttc aagtcacgct gatcaacggc gaaggcgcgg
     2761 aacacgacat cgtctttccc gaccaggatg ccaaatcgcc ccgcgtcacc ggcaagggat
     2821 cgagtacgac gatcgctttc cgcgcgacga aatccggcga cttcatctac ctctgcagcg
     2881 tgccgggcca tcagttggca ggcatgcaag gtcaattcct ggtcacgccg cgcccgacgc
     2941 cacaaaccgt cgtcgaggcg gacatctccc gggaggcaac cgacttgtcg ccacctatcg
     3001 gcaagcgcga tccgcaaacc gttcgtgtcg acctgttcag ggtcgaaatc gaaggtcgcc
     3061 tcgcggaagg cacgacgttc ggctactgga ctttcaacgg caaggtgccg gggccgttca
     3121 tccgggtgcg tatcggcgac accatcgaca tccacctcaa gaactcggcc gacagctcca
     3181 tgatccattc ggtcgatttc catgcaacca ccggaccagg cggcggcgca gcggcgcttc
     3241 aggtcgatcc gggaaaagaa aaatcgatga cctggaaggc attggtgccg ggactttatg
     3301 tctaccactg tgccacgccg atggtcgccg aacacatcgc caacggcatg tacggactca
     3361 tcctcgtcga gcccgaaggc ggtctgccgc ccgtcgatcg cgagttttat gtgatgcagg
     3421 gcgaaatc
//
LOCUS       DATLJT010000878         1951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2354186,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000878 DATLJT010000000
VERSION     DATLJT010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354186"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VM910_20270"
     CDS             complement(<1..1423)
                     /locus_tag="VM910_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64895.1"
                     /translation="MNIIANPHKLRGFEKHIRVDNVSRRTILKRLGIAGAFVLAAPVM
                     SRTAFAYETGAKDMPHGVVVDPRVFVAIAPDGAVTIVAHRSEMGTGIRTSLPLVVAEE
                     MEADWTRVKVQQADGDEVKYGNQDTDGSRSTRHYLMPMRQIGAAARTMLENAAARRWG
                     VPVAEVKATNHEVVHGASGRRMGFGELAADAAKEQVPAIASLKLKDPKDFRYLGKGEV
                     SIVDLRDITVGAARYGGDIRLPGMKYAVIARPPVTGGKLVSFDGADAMKVSGVEKVME
                     VKGWPWPSKFQPLGGVAVIARNTGAAIKGRDALKIVWDDGANAKYESAAYRAALEGAV
                     RKPGLIVRKEGDVDTALKNADKVIVGEYYLPHLAHVSMEPPVAVADVKGDKAEIWAPV
                     QSPGGTHEDVAKTLGIPEDNVTINVTLLGGGFGRKSKCDFALEAALLSKALGAPVKVQ
                     WTREDDIRHDFLHTVSVERIEAGL"
     gene            complement(1426..1878)
                     /locus_tag="VM910_20275"
     CDS             complement(1426..1878)
                     /locus_tag="VM910_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI64896.1"
                     /translation="MIKLNINGKEQSWDGDPDLSLLWYLRDEAGLTGTKFGCGEAYCG
                     SCTVLVDKEAVRSCITTVSDVVGRQVTTIEGLHPTGDHAVQKAWRQLNVPQCGYCQAG
                     QIMQAAALLMDNPKPSHEQIKEAMSGNICRCGCYQRIENAVHLASTGV"
BASE COUNT          315 a          636 c          588 g          412 t
ORIGIN      
        1 cgaggccggc ctcgatccgc tccacggaga cggtgtggag gaagtcgtgg cggatgtcgt
       61 cctcccgcgt ccattgcact ttcaccggcg cacccagcgc ctttgagagc agggccgcct
      121 ccagcgcaaa gtcgcatttc gatttgcggc cgaatccgcc gccaagcagc gtgacgttga
      181 tggtcacatt gtcttcgggg attccgagag tctttgcgac gtcctcgtgc gttccgcccg
      241 ggctctgtac cggcgcccag atctcggcct tgtcaccctt gacgtcggca accgctaccg
      301 gcggctccat gctgacatgg gcgagatgcg gcaggtaata ttcgccgacg atcaccttgt
      361 cggcgttctt caaggcggtg tcgacgtcgc cttccttgcg cacgatcaat cccggcttgc
      421 gcaccgcgcc ctcgagtgct gcgcggtaag cggccgactc gtatttcgca ttggcgccgt
      481 catcccagac gatcttcagc gcgtcgcggc ccttgatcgc ggctcccgtg ttgcgtgcaa
      541 tcacggcaac gccgccgagc ggctggaatt ttgacggcca cggccatccc ttcacctcca
      601 tgaccttctc gacgccggag actttcattg cgtcggcgcc gtcgaatgag acgagctttc
      661 cgccggtcac aggcggccgc gcgatgacgg catatttcat gcccggcagg cgaatgtcgc
      721 cgccataacg cgcagcgccg acggtgatat cgcgaaggtc cacgatgctg acctcgcctt
      781 tcccgagata gcggaagtcc ttcgggtcct tgagtttcag gctcgcgatg gccggcacct
      841 gctcctttgc tgcatccgcg gcaagctcgc caaagcccat acgacggccg cttgctccgt
      901 ggaccacttc gtggttggtg gcctttacct cggccaccgg cacgccccag cgcctggcag
      961 cggcattttc cagcatggtg cgggctgcgg cgccgatctg tcgcatcggc atcagatagt
     1021 gccgcgtgct gcgcgagccg tcggtatcct ggttgccgta cttcacttcg tcgccatcgg
     1081 cctgttgcac cttgacgcgt gtccaatcgg cttccatctc ctcggcgacg acgagcggca
     1141 ggctggtgcg gataccggtc cccatctcgg aacgatgagc cacgatggtg accgctccgt
     1201 ccggcgcgat ggcgacgaac acgcgcggat cgaccacgac gccgtgcggc atgtctttcg
     1261 cgccggtctc gtaggcaaag gcggtccgcg acatcacggg cgcggccaac acgaaggcgc
     1321 cggcaatgcc gagacgctta aggattgtgc gccgcgaaac gttatcgacg cggatatgtt
     1381 tttcgaagcc gcgaagcttg tgcggatttg cgatgatgtt catggtcaca ctcccgtcga
     1441 agcgagatgc acggcattct cgatgcgctg gtaacagccg cagcggcaga tgttgcccga
     1501 catcgcttcc ttgatctgtt cgtgggaggg cttcgggttg tccatcaaca gcgccgccgc
     1561 ctgcatgatt tgcccggcct ggcaatagcc gcattgcggg acattgagct gccgccaggc
     1621 tttttgcacg gcgtgatcgc cggtgggatg cagcccttcg atcgtcgtga cctgccggcc
     1681 gacaacatcc gagaccgtgg tgatgcagga gcgtactgcc tccttgtcga ccagcacggt
     1741 gcaggagccg cagtaagcct cgccgcagcc gaatttcgtg ccggtcaatc ctgcttcgtc
     1801 gcgcagatac cagagcagcg aaagatccgg gtcgccgtcc cagctttgct ctttaccgtt
     1861 gatgttcaat ttgatcatga tcgtccctcg ctcttcttaa gctgttgatg tcctatcggg
     1921 tgcaagccag aacacatttc tatcggcttg c
//
LOCUS       DATLJT010000879         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3504155,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000879 DATLJT010000000
VERSION     DATLJT010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504155"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VM910_20280"
     CDS             <1..891
                     /locus_tag="VM910_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64897.1"
                     /translation="GLYFSLVGIGLNLVFGVMRIVNLAHGDVLMLGAFVALGVVALAG
                     IDPLFAVPLAFVVFVLAGVLLYWVLVPRLQGSANPEMLSIILFFGLSQVIEAITTIFA
                     GTSERSIQSRMLGTVFTTIKVKWFGGKPGGSGPIQIFGQSFPAPWVIAAVTSLIAIGF
                     VYLYLYRTRLGTLTRAVMSRRDEAFASGIDVDRVSAAAFGIGLGLAAVAGVFAPFMFG
                     SVTPAFGADATVTSFAIVVLGSLGNPLGTALGGIVYGVCYMLVQTYLSSWADLLPYVL
                     LILILLLRPSGLLGRRVRVA"
     gene            884..1804
                     /locus_tag="VM910_20285"
     CDS             884..1804
                     /locus_tag="VM910_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI64898.1"
                     /translation="MPSAIKHTLFILAPLVALFAVLPGVYENHLLLFNFVIFLILAQG
                     VNIIYGFTGYLPFGYVGFFGAGAYGFAIMVMHLQSPAIPAVLVAGLVGVALGLLLTPL
                     LRLSGAYFAIANLAASLAVLHFVANPALESITKGPYGVSLTGIFNPAHAYVAALVVLA
                     LTVGAVVYLKNSSFGLALQAVREDAVSASMAGVNVVKMRVVAWLASALVAGLAGGIYA
                     WYVSVFYPDNVFSGDFSIFAIVFALFGGVATITGPIVGVIILYGIYNLIGFTTPQYFQ
                     LIYGLLIMGLVLFLPAGLVSLATRRGWHVP"
     gene            1794..2525
                     /locus_tag="VM910_20290"
     CDS             1794..2525
                     /locus_tag="VM910_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64899.1"
                     /translation="MSPESAPILNVEKLVKRFGGFYALDGLSFHVVPGEILGLVGPNG
                     SGKTTAINVVSGLYAPDGGEVTLDGQAIGGIASHRLVHRGINRTFQVPKPFLSLTVRE
                     NILVALTHGRASVTPPTMAELLEEYRLGGVADRPAADLNSAQQKMLDLTRALATRPRL
                     LLLDELAAGLNPAELDWIAGRIKALAAEGMAIIVVEHLMGFIEQITDRVIVLNAGKEI
                     FEGTLAAAVREPQVIEVFLGGEHAA"
     gene            2515..3234
                     /locus_tag="VM910_20295"
     CDS             2515..3234
                     /locus_tag="VM910_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64900.1"
                     /translation="MPPDAAPLLNAAGVEAGYGTMQVLWGVDLDVRAGETVLLLGANG
                     AGKTTFLKSLVGLVEPRRGSIRLAGEDITHMRSSDRMKLGMTYMSELAVFPDLSIEEN
                     IRVGAQALGHADPAARADELYGLFPVLRDKRRDAASSLSGGQRKMLGIAKALAAEPKL
                     LVMDEPSAGLSPLFVKEVIRILTNLQGRGLALLIAEQNIGFLEVATRVFVLEGGHIRF
                     SGTVAEMSGNEDLHRAYFGLK"
     gene            3234..4574
                     /locus_tag="VM910_20300"
     CDS             3234..4574
                     /locus_tag="VM910_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HVI64901.1"
                     /translation="MRTIRIGSGAGYSGDRIEPALELAEKGDIDYLVFECLGERTVAL
                     AQQARMKNPEAGFDPLMEERMRAVLPLCAAKGIKVVTNMGAANPEAAAKKTAEIARSL
                     GLPSLKIAAVVGDDVLDACKAGDFPILEFDGSIKKLGNRLISANAYLGAEPMAAALAR
                     GADVVVTGRASDPALFLAPMIHAFGWAMDDWNLLGQGTVAGHLLECAGQITGGYFADP
                     GYKDIPDLARLGFPIGEVGEDGSLIVTKVAGSGGAVTAQTCREQLLYEVHDPMKYFQP
                     DVVADFSQVTVEEIAKDRVRVGGGRGSKRTDTLKVSVGYVDSFIGEGQISYAGPGALA
                     RGRLALDIVRERLKLTSVAASELRFDLIGVDSLHGPLVSAHANEPYEVRIRVAGRTES
                     LREAVRIGNEVETLYTNGPAGGGGAWKSARDVVAVASVLLPRELARTEVRFVET"
BASE COUNT          747 a         1428 c         1540 g         1024 t
ORIGIN      
        1 gggctgtatt tcagcctggt cggaatcggc ctcaatctcg tgttcggtgt catgcgcatc
       61 gtcaaccttg cgcatggcga cgtgttgatg ctcggcgcct ttgtcgcgct cggcgtggtg
      121 gcgctcgcag gcatcgatcc cttgtttgca gtgccgctcg ccttcgtcgt gttcgtgctg
      181 gcgggcgtct tgctctattg ggtgctggtg ccgcgcctgc aaggttcggc caatccggaa
      241 atgctgtcga tcatcttgtt cttcggcctg tcgcaggtga tcgaggccat caccacgatt
      301 ttcgccggca ccagcgaacg ctcgatccaa agccggatgc tcggaactgt gttcacaacc
      361 atcaaggtca aatggttcgg cggcaaaccg ggcggctccg gcccgatcca gatttttgga
      421 cagagttttc ccgcgccctg ggtgattgcg gcggtgacga gcctgatcgc gatcggattt
      481 gtctaccttt atctctaccg cacacggctc ggcacgttga cgcgcgcggt gatgtcgcgc
      541 cgcgacgagg ccttcgcgag cggtatcgat gtcgatcgcg tctcggccgc cgccttcggc
      601 ataggtctgg ggttggctgc ggttgcgggt gtgttcgcgc ccttcatgtt cggttcggtg
      661 acgccagcct tcggcgccga tgccaccgtc acatcgttcg cgatcgtggt gttgggttcg
      721 ctgggcaacc ccttgggcac tgcgcttggc ggcatcgttt acggcgtatg ctacatgctg
      781 gtgcagacct atttgagttc gtgggcggac cttctgcctt acgtgctcct gatcctgatc
      841 ctgctgttgc gtccgagcgg cctgctcgga aggcgggtgc gcgttgccta gcgcgatcaa
      901 acacacgctg tttatcctcg ccccgctggt cgcgttgttc gcggtgttgc ccggcgtcta
      961 cgaaaatcac cttttgctgt tcaatttcgt gattttcctc atcctcgccc agggcgtaaa
     1021 tatcatctac gggtttaccg gctatctgcc gttcggctat gtcggttttt tcggcgcggg
     1081 cgcgtacggc tttgcgatca tggtgatgca tttgcagtcg ccggcgatac cggcggtgct
     1141 ggttgcgggc cttgtcggcg tcgcgcttgg actgctgctc acgccattgc tcagactgtc
     1201 cggcgcctat tttgcaatcg ccaatctcgc tgcatccctt gcggtgttgc atttcgtcgc
     1261 caatccggcg ctggaaagca tcaccaaggg gccctacggc gtttcgctga ccggcatctt
     1321 caatcccgca cacgcttatg tggcagctct cgttgtgctc gccttgactg tcggtgccgt
     1381 cgtttacctg aaaaattcca gtttcggtct ggcattgcag gcggtacgag aagacgcggt
     1441 ctccgcttcg atggccggcg tcaacgtcgt aaagatgcgc gttgtcgcct ggctcgcctc
     1501 ggcgttggtt gccggcctcg ccggcggcat ttatgcctgg tacgtctcgg tattctatcc
     1561 cgacaacgtg ttctcgggcg atttctcgat ctttgccatc gtgttcgcgc tgttcggcgg
     1621 tgtcgccacc atcaccggtc cgatcgtcgg cgtcatcatc ctctacggca tctacaatct
     1681 gatcggtttc accacgccgc aatatttcca gttgatctac ggccttctga tcatgggact
     1741 ggtgctgttc ctgcccgctg gcctggtttc gctggcaacg cgcagggggt ggcatgtccc
     1801 ctgagtccgc accgatcctc aacgtcgaga agctcgtgaa gcgctttggc ggcttctatg
     1861 cgctcgatgg tctgagcttt catgtcgttc ccggagagat tttggggctg gttggaccca
     1921 acggatccgg caagaccacg gcgatcaatg tcgtctcggg cctctatgct cccgacggcg
     1981 gcgaggtcac gctcgatggc caggcgatcg gcgggatcgc gtcgcaccgg ctggttcatc
     2041 gcgggatcaa ccgcacgttc caggtcccaa agccatttct gtcgttgacg gtgcgcgaga
     2101 atatcctggt cgcgctcacc catggcagag cctcggtgac gccgccgacc atggccgaat
     2161 tgctggagga atatcgcctt gggggagtgg ccgaccgccc ggcggccgac ctcaacagcg
     2221 cgcagcagaa gatgctcgat ttgacgcgcg cgctcgcgac ccgtccgcgc ctgttgttgc
     2281 tcgacgaact cgcggccggt ctcaatcctg ccgaactcga ttggatcgca ggacgcatca
     2341 aggcgctcgc cgcagaggga atggcgatca tcgtggtcga gcatctgatg ggctttatcg
     2401 agcagatcac cgaccgcgtc atcgtcctga atgccggcaa ggaaatcttc gaaggcacgc
     2461 ttgcggcggc agtcagggag ccccaggtga tcgaggtgtt cctgggaggc gagcatgccg
     2521 cctgacgccg cgccactgct gaatgccgcc ggcgtcgagg ctggctatgg caccatgcag
     2581 gtgctgtggg gcgtcgatct cgatgtgcgt gcgggcgaaa ccgtgctgct gttgggggcc
     2641 aatggcgcag gcaagaccac tttcctgaaa tcgctggtcg gtctggtcga gccacggcgc
     2701 ggctcgatca ggcttgcggg cgaggacatc acgcacatgc gctcaagcga ccgcatgaag
     2761 cttggcatga cctatatgtc ggagcttgcg gtgttccccg acctgtcgat cgaggagaac
     2821 attcgcgttg gcgcacaggc gttgggacat gccgacccgg ccgcccgcgc cgacgagctc
     2881 tatggtttgt ttccggtgct gcgtgacaag cgccgcgatg ccgcctcgag cctctccggc
     2941 gggcagcgaa aaatgctcgg cattgccaag gcgctcgccg ccgagccgaa gctgctggtg
     3001 atggacgaac cttcagccgg gctgtcgccg ctgttcgtca aggaagtgat ccggatcctg
     3061 accaatttgc agggcagggg gctggcgctc ttgattgccg agcagaatat cggtttcctt
     3121 gaggtggcaa cccgcgtgtt cgtgctcgaa ggcggccaca tccgtttttc cggcacggtt
     3181 gccgagatga gcggcaatga agatttgcac cgcgcctatt ttggactgaa gtgatgcgga
     3241 ccattcggat cggctcaggg gccggttatt cgggcgatcg tatcgaaccg gcgctcgagc
     3301 tcgccgagaa gggcgacatc gattacctcg tgttcgaatg cctgggcgag cgcacggtgg
     3361 cgctggcgca gcaggcgcgc atgaagaacc cggaagccgg ctttgatccg ctcatggaag
     3421 agcggatgcg cgcggtgctg ccgctctgcg cggccaaggg catcaaggtc gtcaccaata
     3481 tgggtgcggc caacccggaa gccgcggcga agaagaccgc cgaaatcgcg cgatcgctcg
     3541 gactcccttc gctgaagatc gcggccgtcg tcggcgacga cgttctggat gcttgcaagg
     3601 cgggcgattt tccgattctc gagttcgacg gcagcatcaa gaagcttggc aaccggctga
     3661 tctcggccaa tgcctatctt ggcgcggaac cgatggcggc cgcgctggcc cgcggagccg
     3721 acgtagtggt cacgggccgt gcgtcggatc ccgcgctgtt cctggcgcca atgattcacg
     3781 ctttcggctg ggcgatggac gactggaatc tgcttgggca gggaacggtc gcaggacacc
     3841 tcctcgaatg cgctggccag atcaccggcg gctatttcgc cgatcccggt tacaaggaca
     3901 ttcccgatct ggcccgtctc ggctttccga tcggcgaggt cggcgaggac ggctcgctga
     3961 tcgtcaccaa ggttgcgggc tccggcggcg cagtaaccgc gcagacgtgc cgggagcaat
     4021 tgctctacga ggtccacgat cccatgaaat acttccagcc cgatgtggtc gcggactttt
     4081 cgcaagtcac ggtcgaggag attgcaaagg accgcgtgcg cgtcggtggc ggccgcggca
     4141 gcaagcggac cgacacgctg aaggtttcgg tcggctatgt cgacagtttc atcggcgagg
     4201 ggcagatctc ctatgccggc cccggtgcgc ttgcgcgcgg caggctggcg ctggacatcg
     4261 tccgcgagcg gttgaagttg accagcgtgg ccgcgagcga gttacgcttc gacctgatcg
     4321 gtgtcgactc cctgcacggg ccgcttgtgt ccgcgcatgc caacgaaccc tatgaggtgc
     4381 ggatccgcgt ggccggccgc accgagagcc tgcgcgaagc cgtgcggatc ggcaacgagg
     4441 tcgagacgct ctataccaat ggccccgcgg gcggcggcgg cgcctggaaa tcggcgcgcg
     4501 atgtggtggc agttgcgtcc gtgctgcttc cgcgcgagct tgccaggacg gaggttcggt
     4561 ttgtggagac gtgaaccgat gaagctgcgc gagatcgccc attcccgcac cggcgacaag
     4621 gggaacacct cgaatatctc ggtgatcgca tacgatgcga aacattaccc gctgcttctg
     4681 gagcacgtta cggccgagcg cgtgaaatcg cattttgccg gcgttgtcga aggcgaggt
//
LOCUS       DATLJT010000880         6568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1435983,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000880 DATLJT010000000
VERSION     DATLJT010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435983"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="VM910_20305"
     CDS             complement(<1..2159)
                     /locus_tag="VM910_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64902.1"
                     /translation="MNKHVAPQINRRAFVIGSAAVGAGLALGFDLPFGGPTVVRAADG
                     SPEINAWVVIRPDDTVVIRIARSEMGQGTLTGLSQLVAEELECDWTKVVTEFPTPGQS
                     VARKRVWGDFSTGGSRGIRTSQEYVRKGGATARMMLIQAAANEWKVPVAECTAANSVI
                     THTPSGKTTTYGKVVEAAAKLTPPTEVKLKDPKDWKLVGKGVKRLDTPDKVTGTTVYG
                     IDIKLPGMLNAAIADCPVTGGKVKSFDEARIAGMKGVKKVVKVGDTAVAVVADTWWHA
                     KTALDALPIVWDEGANAKVSSASIAAWLSEGLDSGPAYVGNENGDVKAALANAAKKVE
                     AVYNYPYQNHATMEPLNATALYKADKCEVWCGTQNGEAAFAAVLEVSGLPADKCDVHK
                     LMLGGGFGRRGQTDYVRQAVAIAKQMPGTPIKLLWSREEDMTHGRYHPITQCKMTAGF
                     DADNNLTALHMRISGQSIQFTLRPEGLVNGKDPSTFAGLNPSGDAAFGYTIPNLLIDH
                     SMRNPHIMPGFWRGVNVNHNAIYVESFMDELAQAVGQDPLEFRRKLLKDHPKHLAVLN
                     AVAEKVGWGTPPPQGVSRGIAQFMSYGTYVAGVAEISVIDGNKIKVHRIVAAVDSGYA
                     VNPEQIARQASGSFIYGLTGLFYGGCTVKDGRIEQTNFDTYNSMRINEAPKVETIVMP
                     SGGFWGGIGEPTIGVAAPAVLNAYFAATGKRIRSVPLR"
     gene            complement(2171..2620)
                     /locus_tag="VM910_20310"
     CDS             complement(2171..2620)
                     /locus_tag="VM910_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI64903.1"
                     /translation="MANLTINGKTFTLDVEPDTPLLWAIRENAGLTGTKYGCGIAQCG
                     ACTVHIDGVAMRSCGVSVGDAAGKQITTIEGLGTNGALHRVQQAWIENDVPQCGYCQS
                     GMIMAVVALLKDKPKPTDQDIDEAIANICRCGTFQQVREAIHAAANA"
     gene            complement(2790..4190)
                     /locus_tag="VM910_20315"
     CDS             complement(2790..4190)
                     /locus_tag="VM910_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64904.1"
                     /translation="MFVDRNAVKGQPIPVTDGLPAERRRWAIAAIFTALAMASLDTAI
                     ANVALPAISANLRVNPAEAVWVVNVYQIALVATLLPLGALGEIVGHQRIYLGGLVLFT
                     LASVGCALAWSLESLLIARTLQGLGASGIMSVNTALVRFVYPSRMHGRGFGHNALVVA
                     TAFTFGPTIAPAILAIGSWPWLFAVNLPFGLIAIAIGLKTLPSTPRAVHAFDFPGAAL
                     AAACLGLFIMAIGGAAHQAEPSLVLIALATGLVFGFLLARRHADHPAPMLPIDLFRRP
                     LFALSAATAVCSFAVQGLSFVALPFYFEDILQRSQVETGFFMTPWPLVVAIMAPIGGR
                     LADRFSAGILGGLGLILLGLGMLLLATLPANPGVANIVWRMVICGIGFGLFQTPNMKA
                     LMSSAPLHRSGSASGMVATARLIGQTTGAALAALCFSLAGRDGATLALGLGAAFGGLG
                     SVMSFLRLAVAPQGKA"
     gene            4415..6190
                     /locus_tag="VM910_20320"
     CDS             4415..6190
                     /locus_tag="VM910_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVI64905.1"
                     /translation="MDGQVLSRRIEAAQSGVEGRRRGAIAGVWVVLLLAILGFLVVYP
                     VLTLLLGALTDTNPVVEGLSLRHLSVANFLTVVANPNVADALANTLLACGGGTAIAVV
                     IGLLFSWIVVRTNTPFRGFIAATSILPLFAPPLVAGVAWSILGSPKTGLINTAFKWAG
                     LDWHVDFYSMSGLVFVFGIYYAPYVYMFTSSALRNMDPSLEEAAEISGASAFATLFSV
                     TFPLIMPAIVSGMLLSFIVMLGIYGVPAVLGAPANINVLTTYIFKLTNWSPPLYNTAA
                     AVAIILMVVTGALVFLQQKVLSGRSYTTVAGKAFRPRTLDLGRWRWFTFSLGLIYLVI
                     VVVLPMLALIVAAFRKFMFIRDIASLFDLRQYSLVHFNAIFDNPLTIRSIYNAVEVGL
                     ITAVVGGALAFAIGYTVHRTHVPGRRLIDLIATLPVAIPGLVIGVAYLWAWIGIPGGL
                     YGTIWILALAFIARFMPDTVKALSTSFLQIHRELEEAAWVCGKGMLTTIASIVLPLAR
                     PGVIASMTLLFVLAIRELGSSLFLYTSNTMVMSVLLLDYYEGGNIGKTAAFSLVQTVL
                     LGALIGGANWLARSAAQGGVAQRIR"
     gene            6213..>6568
                     /locus_tag="VM910_20325"
     CDS             6213..>6568
                     /locus_tag="VM910_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI64906.1"
                     /translation="MIRKIFAAMAGIGLGLALGGTHVQAEENFGSPELIAAAKAEGKL
                     VLYTANYAEVEQDVIREFNKRFPEIKVEMVRAPGGQLITRVKTEAAAGKLIADIVDHS
                     DRALMLPLADLFQDYAP"
BASE COUNT         1152 a         2073 c         2065 g         1278 t
ORIGIN      
        1 cgcaagggaa cagatcggat gcgcttgccg gtcgctgcga aataggcgtt gagcaccgcg
       61 ggtgccgcaa ccccgatggt gggttcgccg atgccgcccc agaatccgcc gctcggcatc
      121 acgatcgtct cgactttcgg cgcctcgttg atccgcatcg aattgtaggt gtcgaaattg
      181 gtctgctcga tgcggccgtc cttgacggtg cagccgccgt agaacaagcc ggtcaggccg
      241 tagatgaacg agcccgatgc ctggcgggcg atctgctccg gattgaccgc ataaccggaa
      301 tcgaccgcgg ccacgatgcg atgcaccttg atcttgttgc cgtcgatcac cgaaatctcg
      361 gctacgccag cgacataggt gccatagctc atgaactggg cgatcccgcg cgaaacgcct
      421 tgcggcggcg gggtgcccca accgaccttc tcggctacgg cattgagcac ggcgaggtgc
      481 ttgggatggt ccttcaacag cttgcggcgg aactccagcg gatcctgtcc gaccgcttgc
      541 gccagctcat ccatgaagct ttcgacgtaa atcgcgttgt gattgacgtt cacgccgcgc
      601 cagaaacccg gcatgatgtg cggattgcgc atcgaatggt cgatcaggag attggggatc
      661 gtatatccga acgccgcgtc tcctgacgga ttgagcccgg cgaaggtgga cggatccttg
      721 ccgttgacca gtccttcggg acgcagcgta aactggatcg actgtcctga gatccgcata
      781 tgcagcgccg tcagattgtt gtcggcatcg aaaccggccg tcatcttgca ttgcgtgatt
      841 ggatgatagc ggccgtgcgt catgtcttcc tcgcgcgacc acaacagctt gatcggtgtg
      901 cccggcatct gcttggcaat cgcgaccgcc tgccggacat aatcggtctg gccgcgccgg
      961 ccaaaaccgc cgcctagcat cagcttgtgg acgtcgcatt tgtcggcagg caggccggat
     1021 acctccagca cggcggcgaa cgccgcctcg ccgttttgcg ttccgcacca gacctcgcac
     1081 ttgtccgcct tgtagagcgc ggttgcgttt agcggctcca tggtggcgtg gttctggtag
     1141 ggatagttgt agaccgcttc gaccttcttt gccgcgtttg ccagcgcagc cttcacgtcg
     1201 ccgttttcat tgccgacata ggccgggccg gaatcgaggc cctcgctgag ccacgccgcg
     1261 atcgaagccg aactgacctt ggcgttggcg ccttcgtccc agacgatcgg cagcgcatcg
     1321 agcgcagttt tggcgtgcca ccaggtgtcg gcgacgacgg caaccgcggt gtcgcccacc
     1381 ttcaccacct tcttcacgcc cttcattcca gcgatcctgg cttcgtcaaa gctcttcacc
     1441 ttgccgccgg tcaccgggca atccgcaatc gcggcgttga gcatgccggg cagtttgatg
     1501 tcgatgccat agacggtcgt gcccgtgacc ttgtcggggg tatcgagccg cttcacgccc
     1561 ttgccgacga gcttccagtc cttcggatcc ttgagcttga cctcggtcgg cggcgtcagt
     1621 ttcgcggcgg cttcgaccac tttgccatag gtcgtggttt tgcctgacgg cgtgtgggtg
     1681 atgacgctgt tggcggctgt gcattcggca accggaacct tccattcgtt cgcggccgct
     1741 tggatcagca tcatgcgtgc cgtggcgccg cccttgcgca cgtattcctg cgaggtgcgg
     1801 atgccgcggc tgccgccggt cgagaaatcg ccccagacgc gcttgcgggc gacgctctgg
     1861 ccgggtgtcg gaaattccgt gacgaccttc gtccagtcgc attcgagttc ttccgcgacg
     1921 agttgcgaga ggcctgtgag tgtgccttgg cccatctccg agcgggcaat gcggatcacc
     1981 acggtatcgt cggggcggat caccacccag gcgttgattt cgggcgaacc gtcggcggca
     2041 cggacgacgg tcgggccgcc gaagggaaga tcgaagccga gcgcaagccc ggccccgact
     2101 gcggcggagc cgatcacgaa ggcacggcgg ttgatttgcg gtgcgacatg cttgttcatg
     2161 gtctgcctcc tcacgcgttc gcggcggcgt ggatcgcctc gcgcacctgc tggaaggtgc
     2221 cgcagcggca gatattggcg atggcctcgt cgatgtcctg atcggtcggc ttgggcttat
     2281 ccttcagcag cgccaccacc gccatgatca tcccgctctg gcaataaccg cattgcggaa
     2341 cgtcattctc gatccaggct tgctgtaccc tgtgcagggc cccattagtg ccgagacctt
     2401 cgatcgtggt gatctgcttg cctgcggcat cgccaacact gacgccgcat gagcgcatcg
     2461 caacgccatc gatatgaacc gtgcaggcgc cgcattgtgc gatgccgcaa ccgtatttgg
     2521 tgccggtcag tccggcgttc tcgcggattg cccaaagcaa aggcgtatcc ggctcgacat
     2581 cgagcgtgaa ggttttgcca ttgatagtga ggtttgccat cgcggtcccc tgattggtcc
     2641 aatcctccga ttggacgaca cggggagtgt gagcgcgaaa aatgcggcga gttcaaatca
     2701 agataaccgg cgcatgcgac aggatgatat ctggtctgcg caaatgcgag gatgagatcc
     2761 cggccttcgg ggttagagcg ctttgctgat tacgccttgc cttgcggtgc gaccgccaat
     2821 cgcaggaagc tcatcacgct gccgaggccg ccaaaagcgg cgccgagccc aagcgccagc
     2881 gttgcgccgt cgcggccggc gaggctgaag cacagtgccg caagcgctgc tcccgtggtc
     2941 tgcccgatca accgcgccgt agccaccatg ccgcttgcgc tgccgctgcg gtgtagcggc
     3001 gcactcgaca tcagcgcctt catattcggc gtctggaata aaccgaagcc gatcccgcag
     3061 atcaccatcc gccatacgat gttggcgacg ccgggattgg cgggaagcgt tgccaacagc
     3121 aacatgccga ggccaagcag gatcagtccg aggcccccga ggatgcctgc ggaaaatcgg
     3181 tccgccagac gaccgccgat cggcgccatg atcgcgacca ccaacggcca cggcgtcatg
     3241 aagaatccag tctccacctg cgagcgctgc aggatgtcct cgaaataaaa cggcagggca
     3301 acgaacgaca ggccctgtac ggcaaacgag cagacggcgg tcgcggccga caaggcaaac
     3361 agcggtcgcc ggaacaggtc gatcggcaac attggcgccg gatgatcggc gtgtcggcgc
     3421 gcgaggagga agccgaacac caggccggtc gcgagcgcga tcagcaccag gctcggctct
     3481 gcctgatgcg ccgcgcctcc aatggccatg atgaagagac cgaggcaggc ggccgccaat
     3541 gcagcaccgg gaaagtcgaa agcatgcacg gcccgtggcg tcgacggcag cgtcttcaac
     3601 ccgatcgcga tcgcaatgag gccgaaggga agattgactg cgaacagcca cggccaggac
     3661 ccgatcgcca ggattgcggg cgcgatggtc ggtccgaagg tgaaggcggt cgcgacgacc
     3721 agcgcgttat ggccaaatcc gcgaccgtgc atgcggctcg gatagacaaa gcgcacgagg
     3781 gccgtgttca cgctcatgat gccgctggcg ccaagcccct gcagcgtgcg ggcgatcaag
     3841 aggctttcca gcgaccaggc gagagcgcag cccacggacg caagcgtgaa cagcacgaga
     3901 ccgccgagat agatgcgctg atggccgacg atctcgccga gcgcgccaag cggcagcagc
     3961 gtcgcaacca gagcgatctg atagacattg accacccaca ccgcctcggc ggggttgaca
     4021 cgtaaattgg cggaaatggc ggggagtgcg acattggcga tcgccgtatc cagcgatgcc
     4081 atcgcaagcg cggtgaagat cgcggcaatg gcccagcgcc gccgctccgc aggcaaaccg
     4141 tcggtgaccg gtatcggttg gcccttcacg gcgtttcgat cgacaaacat gctcgaatca
     4201 attcttcgcc ttgaaggccc ggtgcgttcc gggaggcatt tagtacgccc gggcggcatc
     4261 ccccagtcgc atggacgcat aatggccatg cggcagggcc ggcccgcgat ttcgggccga
     4321 ctttcggcta cttccgggct gccggccgat cgactaggct tccttccaac gcggtcaaga
     4381 tcacgaacga agcaccgccg gaagggacag cgcgttggac ggacaggttt tgagccggcg
     4441 catcgaggct gcgcaaagcg gcgtcgaggg caggcgccgg ggcgcaatcg ccggcgtctg
     4501 ggtcgtcctg ttgctcgcga tcctgggttt tctggtcgtt tatcccgttc tcacgctgct
     4561 gctcggcgcg ctcaccgaca ccaatccggt ggtcgagggc ctgagcctgc gtcatctgtc
     4621 agtcgcaaat ttcctgaccg ttgtggccaa tccgaacgtc gcagacgcgc ttgccaacac
     4681 gctgcttgcc tgcggcggcg ggacagcgat cgcggtcgtc atcgggctgt tgttttcctg
     4741 gatcgtggtg cgcaccaaca cgccgttcag gggctttatc gccgcgacca gtatcctgcc
     4801 gctgtttgcg ccgccgctcg ttgcgggcgt tgcatggtcc atcctgggat cgccgaagac
     4861 ggggttgatc aacaccgcct tcaaatgggc gggcctcgac tggcatgtcg acttctattc
     4921 gatgtccggc ctcgtgttcg tgttcggcat ctattacgcg ccctatgtct acatgttcac
     4981 ctcgtcggcg ctgcgtaaca tggaccccag cctcgaggag gctgcggaga tttccggcgc
     5041 gagcgccttt gcgacgctgt tctcggtgac attcccgttg atcatgccgg cgatcgtctc
     5101 gggcatgctg ctctccttta tcgtgatgct cggcatctat ggcgtgcccg cggtattggg
     5161 tgcgcccgcc aacatcaacg tcctgacgac ctatatcttc aagctcacga actggtcgcc
     5221 gccgctttac aacacggccg ccgccgtcgc gatcatcctg atggtggtga ccggtgcgct
     5281 cgtcttcctg cagcaaaagg tcctgagcgg gcgcagttac accaccgtcg ccggcaaggc
     5341 attccggccg cgcaccctcg atctgggccg gtggcgctgg ttcacctttt cgctcgggct
     5401 gatctacctc gtgatcgtcg tggttctgcc gatgctcgcg ctgatcgttg cggcctttcg
     5461 caaattcatg tttatccgcg acatcgccag cctgttcgat ctccggcaat attcgcttgt
     5521 tcatttcaac gccatcttcg acaacccgct gaccatccgc tcgatttaca acgcagtcga
     5581 ggtcgggctg atcacggcgg tggtcggcgg ggcgcttgcg tttgcgatcg gctataccgt
     5641 ccatcgcacc catgtgccgg gccggcgctt gatcgacctg atcgcgacgc tgccggttgc
     5701 gattcccggc ctcgtgatcg gcgtcgccta tctctgggcc tggatcggga ttcccggcgg
     5761 gctctacggc acgatctgga tcctggcgct cgcctttatc gcgcgcttca tgcccgacac
     5821 ggtgaaggcg ctttcgacct catttctgca aattcaccgc gaactcgaag aagctgcctg
     5881 ggtctgcggc aagggtatgc tgacgaccat cgcctccatc gtgctgccgc tggcgcggcc
     5941 gggcgtgatc gcctcgatga cgctgctgtt cgtgctggcg atccgcgaac tcggttcctc
     6001 gctgtttctc tacaccagca acaccatggt gatgtcggtc ctgctgctcg actattacga
     6061 gggcggcaat atcggaaaga ctgcggcatt tagcctggtg caaaccgtgt tgctcggcgc
     6121 tctcatcggc ggggcgaatt ggctggcgcg gagcgccgcg cagggcggcg tagcacaacg
     6181 gatcagataa aaaacaggga gggaaatcgt tcatgatcag gaagattttc gcggcgatgg
     6241 ccggcatcgg cctcggtctt gccctcggcg gcacgcacgt gcaggcggaa gagaatttcg
     6301 gctcgcctga actgatcgcg gccgccaagg ccgaaggcaa gctggtgctc tataccgcga
     6361 actatgcgga ggtcgagcag gacgtcatca gggaattcaa caagcgcttt cccgagatca
     6421 aggtcgagat ggtgcgcgcg ccgggcggcc agctcatcac gcgggtgaag acggaagccg
     6481 ccgcaggcaa gctgattgcc gacatcgtcg accattccga ccgcgcgctg atgctgccgc
     6541 tcgccgacct gttccaggac tacgcgcc
//
LOCUS       DATLJT010000881         9676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1435994,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000881 DATLJT010000000
VERSION     DATLJT010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435994"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="clpX"
                     /locus_tag="VM910_20330"
     CDS             complement(<1..1178)
                     /gene="clpX"
                     /locus_tag="VM910_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HVI64907.1"
                     /translation="MSKVGASDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGIPTPKEICKVLDDYVIGQSHAKKVLSVAVHNHYKRLNHQT
                     KHNDVELAKSNILLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQAADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFSGLEKIISARGRSTSIGFGAQVLAPE
                     DRRTGEIFRHVEPEDLLKYGLIPEFVGRLPVVATLEDLDEASLKKILTDPKNALVKQY
                     QRLFEMENIELTFADEALGAVSRKAIERKTGARGLRSILESILLETMFDLPGLEGVEE
                     V"
     gene            complement(1578..2213)
                     /locus_tag="VM910_20335"
     CDS             complement(1578..2213)
                     /locus_tag="VM910_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HVI64908.1"
                     /translation="MRDPVETYMNLVPMVVEQTNRGERAYDIFSRLLKERIIFVTGPV
                     EDGMSTLVVAQLLFLEAENPKKEISMYINSPGGVVTSGLAIYDTMQFIRPPVSTLCTG
                     QAASMGSLLLAAGEKDMRFTLPNSRIMVHQPSGGFQGQATDIMLHAQEILNLKKRLNE
                     IYVRHTGQSYKAIEDALERDKFLTADQAKDFGLVDKVIDKRPEDPAAAKAA"
     gene            complement(2401..3762)
                     /gene="tig"
                     /locus_tag="VM910_20340"
     CDS             complement(2401..3762)
                     /gene="tig"
                     /locus_tag="VM910_20340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVI64909.1"
                     /translation="MQVTETLSEGLKHEFQISVPASDLDAKADARLVDLKDKVRLNGF
                     RPGKVPVSHLKKVYGRSVMAETIDQTIRDTNTQIFTERGFRLATEPKITMPTEEKAVE
                     ELLSGKTDLTYTVSIEVVPAIKLADFKTFAIEKPVAEVTDAEVDEAIQRIADQNRSYG
                     PKGEGAKAETGDRVTINFKGTINGEPFEGGTGENIQVVIGVGQFIPGFEDQLIGIGSG
                     ETRTLKVSFPKNYASNKLAGQPAEFETTASAIEAPLPTEINDEFAKSLGLESLEKLKE
                     AARGRLAAEFAGATRARLKRALLDKLDESHKFEAPPSLIDEEFNLMWNSIRAEMDSSG
                     KTFADENTTEEAARDEYRTIADRRVRLGLVLSEIGEKNKISVTDDEVSRAVIERARQA
                     PGREKEVWDYYRKNATALAQLRAPIFEDKVVDFILELATVTDKKVSREELFRDDDAEK
                     SAA"
     gene            complement(3927..4011)
                     /locus_tag="VM910_20345"
     tRNA            complement(3927..4011)
                     /locus_tag="VM910_20345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3975..3977),aa:Leu,seq:tag)
     gene            4239..5477
                     /locus_tag="VM910_20350"
     CDS             4239..5477
                     /locus_tag="VM910_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI64910.1"
                     /translation="MPRTASGEERQALKLHAKAATVALQPNQGDTTIWSLEPPAPSPP
                     LRFKRGSRLEITLQNDLPLPIVLNWRGLDGVPAAEPLVAQPPLTPGRSTTFSLPLRHS
                     GSLLCDPRLLGDSTERPSRPLPIIIQETDAISDRDEVFLVEDWRIRGDGTALTPGQPP
                     QDTVPIYSVNGQLIPEIAVRFRERLRLRFINGCQRRVIALKFERYDVRVMALDSQPSE
                     PFMARNGALVLAPDGRADVLIDATDPPGSTSDILLHDGKEARPVARLVTSTAPPVRPE
                     LLPPASALPSNGLPAQLDLRSALRVDLALGGAEWAKPSSFNMSAAPAFRTKTGRVVVL
                     ALSSRAETATVFHLHGHHFRLLDRLDDGWKPYWLDTLAIEPGQTQRIAFAAEYGGRWL
                     LEATATDWAAPRLLRWYSVE"
     gene            5490..6389
                     /locus_tag="VM910_20355"
     CDS             5490..6389
                     /locus_tag="VM910_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI64911.1"
                     /translation="MTKTTSAIRSVKARALVAIVPFDLMGAMDAKFRLLGWQGLIGMA
                     VSGLDMAFWDALGQIAGKPVVELLGGSPRPMLAYDSYGAVDPAADEKHLRRSIEQGFR
                     GIKIKGGYGDAANDERVVKGVRALLGPDIALMIDFNQSLDPEEAKRRIARLAPYDLTW
                     VEEPVPQENLCGHAEVRKSSPIPIQAGENWWFPRGFAEAIAAGASDFIMPDLMKVGGV
                     TGWLRVAAQAEAASIPMSSHLFAEASAHMLAVTPTAHWLEVLDFAGSILASPIRIVDG
                     TLTARGPGLGLEWSETGVSKYLI"
     gene            complement(6395..7894)
                     /locus_tag="VM910_20360"
     CDS             complement(6395..7894)
                     /locus_tag="VM910_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVI64912.1"
                     /translation="MEVLTTAEMERADRFTITAGTPGFALMLSAGQAVAEAANDLVEQ
                     GPILVVAGPGNNGGDGFVAAAELAAQGREVSVILMCERDALKGDAASAARGWKYPVLP
                     FTPQAIGKPALIIDALFGAGLSRPVEGDALEMIEAIKLTGVPVLAVDLPSGVNGTSAA
                     IMGVAVRAVETVTFFRKKPAHLLLPGRQQCGRVRVADIGIDARVLDEIRPRTFENMPQ
                     FWRKSFPVPRSEGHKYARGHALVVSGDIAATGAARLAARGALRAGAGLATVASPRDAL
                     AVNAAALTAVMVRAIDTVVEFAEAFSDRRFNSCVIGPGAGIGKRTIDFVHTALSAQRH
                     LVLDADALTSFADGPDRLFESIKTSQNHEVVLTPHEGEFARLFSDLSNKTPGRSKLER
                     VRAAAERSGAVVVLKGPDTTVAAPDGRATIAANAPPWLATAGAGDVLAGIIAGLLAQG
                     VAAFEAASIAVWMHGEAAREAGPGLIAEDLPETLPAVFRHIYDEFGIDY"
     gene            8379..8717
                     /locus_tag="VM910_20365"
     CDS             8379..8717
                     /locus_tag="VM910_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVI64913.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHAE
                     LYRGAEYIVDFLPKVKIEIVIGDDLVDRAIDAIRRAAQTGRIGDGKIFVSNIEEAIRI
                     RTGESGLDAI"
     gene            8883..>9676
                     /locus_tag="VM910_20370"
     CDS             8883..>9676
                     /locus_tag="VM910_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HVI64914.1"
                     /translation="MKTAKDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDAGMFEEDT
                     FAEGLMFDGSSIAGWKAINESDMCLMPDPVTATIDPFFAETTMVLVCDVLEPTTGEPY
                     NRDPRGIAKKAEAMVKSMGIGDTVFFGPEAEFFVFDDVKYAAEPYNTGFKLDSSEFPT
                     NSDTEYEGGNLGHRIRTKMGYFPVPPQDSVQDMRSEMLGAMAKMGVKVEKHHHEVASA
                     QHELGMKFDTMTLMADHLQIYKYCIHQVAHIYGKTATFMPKPVFGD"
BASE COUNT         1828 a         3098 c         2887 g         1863 t
ORIGIN      
        1 acttcctcga caccctcaag gcccgggaga tcgaacatgg tctcgagcag gatgctttcg
       61 aggatcgaac gcaaccctcg cgcaccggtc ttgcgctcga tcgccttgcg ggagacggcg
      121 ccgagcgcct cgtcggcgaa ggtcagctcg atattctcca tctcgaacag ccgctgatac
      181 tgcttcacca gcgcgttctt cggatcggtc aggatcttct tcagcgaggc ttcgtccaaa
      241 tcctcgagcg tcgccacgac aggcaaacgg ccgacgaact ccgggatcaa gccgtacttg
      301 agcaaatcct ccggctcgac gtgacggaag atctcgccgg tccggcgatc ttcgggggcg
      361 agcacctggg cgccaaagcc gatcgaggtc gaacgtccgc gcgccgagat gatcttctcc
      421 aggccggaga acgcgccgcc gcagatgaac aggatgttgg tcgtgtcgac ctgcaggaat
      481 tcctgctgcg gatgcttgcg gcctccttgc ggtggaaccg aagccaccgt gccttccatg
      541 atcttgagca gcgcctgctg cacgccctcg cccgacacgt cgcgggtgat cgaggggttg
      601 tccgacttgc gactgatctt gtcgatttcg tcgatataga cgatgccgcg ctgcgcgcgc
      661 tctacattgt agtcggccgc ctgcaacagc ttcaggatga tgttctcgac gtcctcgccg
      721 acgtaaccgg cttcggtcag cgtcgtcgca tcggccatgg tgaagggcac gtccagaatc
      781 cgcgccagcg tctgcgcaag gagcgtcttg cccgacccgg tcggaccgat caggagaatg
      841 ttcgacttcg caagctcaac gtcgttgtgc ttggtctggt ggttgagccg cttgtagtga
      901 ttgtgcaccg cgacggagag caccttcttg gcgtggctct ggccgatcac gtaatcgtcg
      961 aggaccttgc agatttcctt cggggtcggg attccgtcac gcgacttgac cagcgaggac
     1021 ttgttctcct cgcgaatgat gtccatgcag agttcgacgc attcgtcgca gataaaaacc
     1081 gtcggacccg cgatcagctt gcggacttcg tgctggctct tgccgcagaa cgagcaatac
     1141 aacgtattct tggagtcgct cgcgccgacc ttactcattc ctgtctccgt ccgccgttcg
     1201 acttcctccg cccgatcgct ccatttgggt taacaccgcc atggaactca gatagagcaa
     1261 attccgtacc aaaaaagacc ttacgccata cttaccgcgc gaatcacggt tttattcgaa
     1321 tcccccgcga tcccgaccga tcactcgtag ccaaccatgc tgacacccga ctatcaagaa
     1381 ttcgctaacc gaccggggcg ttcaacaccg tgacaatacc gtgatttacg gcgttgacac
     1441 gaggtaagct gctgaaatcg gccaagcgtt gcgggattac cacggctgat gagcacgaaa
     1501 gcggaacttt ccgcctgtgc tggcgcctcc ggcggccccc ggacggacag cccgaggcgc
     1561 ggttgcagcc tcgtcggcta agcggccttc gcagccgcgg gatcctccgg gcgtttgtcg
     1621 atcaccttgt cgacaagccc gaagtccttg gcctggtcgg cggtcaggaa cttgtcgcgc
     1681 tccagcgcat cctcgatcgc cttgtagctc tggccggtat gcctgacata gatttcgttg
     1741 agccgctttt tgagattcag gatctcctga gcgtgcagca tgatgtcggt cgcctggccc
     1801 tggaagccac cggagggctg gtgcaccatg atgcgcgagt tcggcagcgt gaagcgcatg
     1861 tccttctcgc cggcggcaag caatagcgat cccatcgagg ccgcctgccc cgtgcacagc
     1921 gtcgagaccg gcggacggat gaactgcatg gtgtcgtaga tcgcaagccc cgatgtcacc
     1981 acgccaccgg gcgagttgat gtacatcgaa atttccttct tcgggttttc cgcttccaga
     2041 aacagtagct gcgcgaccac gagggtcgac ataccgtctt cgaccggacc ggtgacgaag
     2101 atgatgcgct ccttgaggag gcgcgagaaa atgtcatagg cgcgttcacc gcgattggtc
     2161 tgctcgacca ccatcggcac aaggttcatg taggtttcaa cgggatcgcg catgagtcac
     2221 cccaacgttt ccagaaacag ttctcatcta tggaccgaat tgccggcgac aagacctagc
     2281 caaaagagca ggtcgcgcgc tgcaaagaac agttgaagct gattcggcgc aggccgccta
     2341 gtaagtgaaa ccgctgccag accgctttgg ccattcatca ttaattctgc actgacaggc
     2401 tcaggccgcg ctcttttcgg cgtcgtcatc cctgaacagc tcttcgcggc tgaccttctt
     2461 gtcggtcacg gtggcgagtt cgaggatgaa gtcgaccacc ttgtcctcga agatcggagc
     2521 acgaagctgg gccaacgccg tggcattttt gcgatagtag tcccacactt ccttctcgcg
     2581 tcccggcgcc tggcgcgcgc gctcgattac cgcgcggctc acctcgtcgt cggtgacgct
     2641 gatcttgttt ttctcaccga tctccgagag cacgagccca aggcgcactc tccggtccgc
     2701 gatcgtccgg tactcgtctc tggcagcctc ttcagtggta ttctcgtcgg caaaggtctt
     2761 gccgctactg tccatctcgg ccctgatcga attccacatc aggttgaact cttcgtcgat
     2821 cagcgacggc ggcgcctcga atttgtggct ctcatcgagc ttgtccagca gggcgcgctt
     2881 gaggcgcgcg cgcgtcgcgc cggcgaattc ggcggcgagc ctgccgcgcg cggcttcctt
     2941 cagcttttcc agcgattcaa gcccgagcga cttggcgaac tcgtcgttga tctcggtcgg
     3001 cagcggcgcc tcgatggcgc tcgccgtcgt ctcgaactcc gcgggctgac cggcgagctt
     3061 gttgctcgcg tagttcttcg gaaacgatac cttcagagtg cgcgtctcgc ctgaaccgat
     3121 gccgatcaac tgatcctcga agccgggaat gaactgaccg acaccgatca cgacctggat
     3181 gttctcgccg gtgccgcctt cgaacggctc gccattgatg gtgcccttga aattgatggt
     3241 gacgcggtcg ccggtctccg ccttggcgcc ctcgcccttt ggaccatagg agcggttctg
     3301 gtcggcgata cgctggatcg cctcgtcgac ctccgcgtcg gtcacctcgg cgaccggctt
     3361 ctcgatcgcg aacgtcttga agtcggcaag cttgatggcc ggcacgacct caatcgagac
     3421 ggtgtaggtg agatcggtct tgccggagag cagttcctcg accgccttct cctccgttgg
     3481 catggtgatc ttgggctcag tggcaagccg gaagccgcgc tcggtgaaga tttgcgtgtt
     3541 ggtgtcgcgg atcgtctggt cgatggtctc ggccatcacg gagcggccat agaccttctt
     3601 cagatggctg acgggcacct tgcccggacg aaagccgttg aggcggacct tgtccttcag
     3661 atcgaccagg cgtgcgtctg ccttggcatc gaggtccgat gcgggaacgc tgatctgaaa
     3721 ttcgtgtttg agcccttccg agagggtttc ggtgacctgc atggcgtcca atcttcttcc
     3781 gctttcgtcc ggcctgtcgg ccgaaaccgc ttaactcatg tgatcggcgc gcggcaaaag
     3841 ccttgcgtcc gtgttgctcg cgaacccgat ccccaaaagc ggagacaggc tcttcgtgat
     3901 ccctggtgca aacgcctgaa ggcgcttggt gcgggcggag ggactcgaac ccccacaact
     3961 ttcgtcactg gaacctaaat ccagcgcgtc taccagttcc gccacgcccg ctcggttagc
     4021 atcaggttcg gccgcgatgc cgcgggcggc ggcttataac atgagcccgt ccgtccgcag
     4081 caaaaaaatg gcccccttca ggcaccgtcc gacgtcggcc aatgctggac atgatgcgcc
     4141 aaaaatggtc cgcaatcagc ctcatggcaa agcgcaatct acccaccaac cgacgcgagc
     4201 tgttggcccg attaggcgcg ctcacgttga tacctgcctt gcccaggacc gcctccggcg
     4261 aggagcggca agccctcaag ttacacgcca aggccgcgac cgtcgcgctg cagccaaatc
     4321 agggggatac aacgatatgg tcactggagc cgccggcgcc aagcccgcct cttcgcttca
     4381 aacgaggcag ccgacttgaa atcacgctgc agaatgatct tccgctcccg attgtcctca
     4441 actggcgagg tttggacggt gtcccggccg ccgagccgct tgtggcgcaa cccccgctga
     4501 cgcccggacg aagtacaacc ttcagcctac cgctgcgcca ttccggcagc ttgctttgcg
     4561 atccgaggct gctcggcgac agcaccgagc ggccatcccg gccgcttccg attatcattc
     4621 aagaaaccga cgcgatctcc gatcgtgacg aagttttttt ggtcgaggat tggcgcattc
     4681 gcggtgacgg cacggcgctt acccccggtc aaccaccgca ggacaccgtc ccgatctaca
     4741 gcgtcaatgg ccagcttatc cctgaaattg cagtccgctt ccgggaacgg ctcaggcttc
     4801 gcttcatcaa cggctgccaa cgtagggtta ttgcactcaa attcgaacgg tatgacgtcc
     4861 gggtgatggc gctggacagc caaccttcgg aacccttcat ggcgcggaat ggcgcgctcg
     4921 tgctggcgcc ggacggcaga gccgatgtcc tgatcgacgc gaccgacccg cccggctcga
     4981 cctcggacat tctgctgcac gacggaaagg aagcgcgccc ggtggcacgg ctcgtcactt
     5041 cgaccgcccc gccggtacga ccggagctgc tcccgcccgc ctccgccctg ccatcgaacg
     5101 gcctgccggc ccagctcgat cttcgcagcg cgttacgcgt cgacctcgca ctcggcggag
     5161 ccgaatgggc gaagccctca agcttcaaca tgtcggctgc acccgcgttt cgaaccaaga
     5221 caggccgcgt cgtggtactc gctctgagca gccgcgccga gactgccacc gtgttccacc
     5281 tgcacggcca tcacttccgt ttgctcgatc ggctcgatga cggctggaag ccatattggc
     5341 tcgatacgct ggctatagag ccgggccaaa cccagcgcat tgcttttgcc gcggaatatg
     5401 gaggacgatg gctgctggaa gcgacagcga ccgactgggc cgcgccgcga ctcctgcggt
     5461 ggtacagtgt cgagtaaggg gaaacgcaga tgaccaagac cacatccgcc attcgaagcg
     5521 tcaaggcgcg cgcgctcgta gcaatcgtcc cgttcgacct gatgggcgcc atggatgcaa
     5581 aattccggct gctcggctgg cagggtctca tcggcatggc ggtctcgggc ctcgacatgg
     5641 ccttctggga tgcgctcggt caaatcgccg gcaagccggt ggtcgaactg ttgggcggca
     5701 gtccgcgccc gatgctggcc tatgacagct acggcgcggt cgatcctgca gccgacgaga
     5761 agcatctgcg ccgctccatc gagcagggct ttcgcggcat caaaatcaag ggcggctacg
     5821 gtgacgccgc aaacgacgag cgcgtcgtca agggagtgcg cgccctgctc ggccccgaca
     5881 tcgcgctgat gatcgacttc aaccagtcgc tcgatcctga ggaagccaag cgccgcatcg
     5941 cgcgccttgc gccctacgac ctcacctggg tcgaagaacc ggtaccgcag gagaatttgt
     6001 gcggccacgc cgaagtgcga aagtcatcgc cgatcccgat ccaggccggt gaaaactggt
     6061 ggtttccacg cggctttgcg gaagcgattg cggccggcgc aagcgacttc atcatgcccg
     6121 atctgatgaa ggtcggcggc gtcacgggct ggctgcgcgt tgccgcacaa gccgaggctg
     6181 catcgatccc gatgtccagt cacctgtttg ccgaggcaag cgcccacatg cttgcggtga
     6241 cgccgacggc acactggctg gaagtgctgg atttcgcggg cagcattctg gcgagcccga
     6301 tccggatcgt cgacggcacg ctcaccgcgc gggggccggg tctcgggctc gagtggagcg
     6361 agaccggcgt ttcgaaatat ctgatctgat cgcgtcagta atcgatgccg aactcgtcgt
     6421 agatgtgccg gaaaacggca ggcagcgttt ccggcagatc ttctgcaatg aggcccggac
     6481 cggcctcgcg cgcggcttcg ccgtgcatcc acacagcgat gctcgcggcc tcgaaagcgg
     6541 caaccccctg ggccaacaga cccgcaatga tcccggccag cacgtcaccg gcgccggcgg
     6601 tagcaagcca tggcggcgcg ttggctgcga tggtcgcgcg gccatcgggc gccgcaaccg
     6661 tggtgtccgg acccttcagc acgacaaccg caccggagcg ttcggccgcc gcgcgcaccc
     6721 gctcgagctt ggagcggccg ggggtcttgt tgctgaggtc gctgaacagc cgcgcaaact
     6781 ccccttcgtg cggagtgaga accacctcat gattttgcga ggtcttgatg gattcaaaca
     6841 aacgatccgg cccgtcggca aagcttgtca acgcatccgc atccagcacc agatgccgct
     6901 gtgccgacag cgcggtatgg acgaagtcga tcgtgcgctt gccgatgccg gcgcctggtc
     6961 cgatcacgca ggaattgaac cggcggtcgc tgaacgcttc ggcaaattcg accacagtgt
     7021 cgatagcgcg aaccatcacc gctgtaagcg ccgccgcgtt aacggcaagc gcatccctgg
     7081 gcgaggccac cgtggcgagg cctgcgcctg cgcgcagggc gcctcgtgcc gccagccgcg
     7141 cggcgccggt cgccgcaatg tccccggaga cgacgagcgc atgaccgcgg gcatatttgt
     7201 ggccctcgct ccgcggcacc gggaacgact tgcgccagaa ctgcggcatg ttctcgaacg
     7261 tgcgcgggcg gatctcatca agaacgcggg cgtcgatacc gatatcggcc acgcggacac
     7321 gaccgcattg ctggcggccc ggcagcaaca gatgagccgg cttcttgcgg aaaaacgtaa
     7381 cggtttccac cgcgcgcacc gccacgccca tgatcgccgc cgaggtgcca ttgacgccgc
     7441 tcggcaaatc gacggcgagc accggcacgc cggtcaactt gattgcctcg atcatctcga
     7501 gcgcgtcgcc ctcgaccggc cggctgaggc ccgcaccgaa caaggcatca atgatgagcg
     7561 ccggcttgcc gatcgcttgc ggcgtgaaag gcagcaccgg atatttccag ccccgcgcgg
     7621 cggacgccgc atcgcccttg agggcatcac gctcgcacat caggatcacg gagacctcgc
     7681 gtccctgggc tgcgagttcc gccgcggcaa caaagccgtc gccgccatta ttacccggcc
     7741 cggcgacaac caggatcggt ccctgctcga cgagatcgtt ggccgcctcc gccaccgcct
     7801 ggcccgcgct cagcatcaac gcaaagccgg gcgtgccggc ggtgatggtg aaccggtcgg
     7861 cacgctccat ctcggcggtg gtcagaactt ccatgccttg tccctatgcc cttcccgaga
     7921 cctagcacga actctgaaag caaaggccga accaaaccct atcaatttag gtgtaacagg
     7981 gtagtgtagc tgtgcctgca gcgaactgcg gcagtggccg cgcctcaact tgaggcattt
     8041 ttcgggggat attgattccg cccggtcacc ggctgcatat cttttcatct atttgcctat
     8101 ttcgtaggcc tatctattct gaccttgctt cgcggtagca ggcgaaatga ccattaacag
     8161 tctgataacg cgttggaatc cgctaatttt acgaattggc atagagcctg cttttgagga
     8221 agccgcttcg gtttcctcgt gactgaccgt ccgaaggacg cctgtccggc gcgagccgga
     8281 cggtcagaac aggcccgaac caagattttt gcgttcaacg cacggcctta tgacgaagtt
     8341 actattttgc ggtctggaaa tgcccgggag gcgctcaagt gaagaagatt gaagccatca
     8401 tcaagccgtt caagctcgac gaggtgaagg aagcgcttca ggaagtggga cttcagggca
     8461 tcacggtgac cgaggccaag ggctttgggc ggcaaaaggg tcatgccgag ctttatcgcg
     8521 gcgccgagta catcgtggac tttctaccca aggtcaaaat cgagatcgtg atcggcgacg
     8581 accttgtcga tcgggcgatc gatgcgatcc ggcgcgcggc ccagaccggg cggatcggcg
     8641 acggcaagat cttcgtctcc aacatcgagg aggccatccg cattcgcacc ggtgagtccg
     8701 ggctggacgc gatttgacgg tccgcccgat cttggaggcc ccttacattg ctgaaagaac
     8761 atgcaattgc cggtgttgcc ggcactgcaa tattgtcttg aactgcaccc gacgctattg
     8821 agcatttacc acgcggctcg gccgcggccg gatcacgagg acctgtagcc aagaggggta
     8881 tcatgaagac cgccaaagac gtcctgaaat ccatcaagga caacgacgtc aaatacgtcg
     8941 atttgcgttt taccgatccg cgcgggaagt ggcagcacgt aactttcgac gctggcatgt
     9001 tcgaagagga cacgttcgcg gaaggcctga tgttcgacgg ctcgtcgatc gccggctgga
     9061 aggcgatcaa cgagtccgac atgtgcctga tgcctgatcc ggttaccgcg acgatcgatc
     9121 cgttcttcgc cgaaaccacg atggtgctgg tctgcgatgt gctcgagccc accaccggcg
     9181 agccctataa tcgcgacccc cgcggcatcg ccaagaaggc cgaggcaatg gtcaagtcga
     9241 tgggcatcgg cgacaccgtg ttcttcggcc ccgaagccga gttcttcgtg ttcgacgacg
     9301 tgaagtacgc ggccgagccc tacaacaccg gcttcaagct ggattcttcg gaattcccga
     9361 ccaactccga taccgagtat gagggcggca atctcggcca ccgcatccgc accaagatgg
     9421 gctacttccc cgtgccgccg caggactcgg tgcaggacat gcgctccgag atgctcggcg
     9481 ccatggccaa gatgggcgtg aaggtcgaga agcatcacca cgaggtcgcc tctgcccagc
     9541 acgaactcgg catgaagttc gacacgatga cgctgatggc cgaccatttg cagatctaca
     9601 aatactgcat ccaccaggtc gcccatatct acggcaagac cgccactttc atgccgaagc
     9661 cggtgttcgg cgacaa
//
LOCUS       DATLJT010000882         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_57979,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000882 DATLJT010000000
VERSION     DATLJT010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57979"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VM910_20375"
     CDS             complement(<1..169)
                     /locus_tag="VM910_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HVI64915.1"
                     /translation="MAAIDPTARIEPGAVIGQDVAIGPYCVVGPGVKIGDGCRLLAHV
                     HIAGHTTIGPRT"
     gene            complement(178..636)
                     /gene="fabZ"
                     /locus_tag="VM910_20380"
     CDS             complement(178..636)
                     /gene="fabZ"
                     /locus_tag="VM910_20380"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HVI64916.1"
                     /translation="MNKAATTTLEAADIAAILKALPHRYPFLMVDRIIDMRGDEAAIG
                     IKNVTVNEPQFVGHFPGNPVFPGVLLIEGMAQTAGAICVLSQMGGDHPKQVYFLTIDK
                     AKFRKPVVPGDTIEYHVRKIARKKNMWWFRGEAKVAGHTVAEAEVGAMVA"
     gene            complement(633..1742)
                     /gene="lpxD"
                     /locus_tag="VM910_20385"
     CDS             complement(633..1742)
                     /gene="lpxD"
                     /locus_tag="VM910_20385"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVI64917.1"
                     /translation="MTEPFFFNRGRGLSVREIAALTGAAPRPGSDLDRRITGIAALDQ
                     AAPSDLAFLEKAKYAHQLSTSGAGACLTTERYAGRAPAHVSVLCVAEPYRAFVEVTRA
                     LFPDALRPSSLFEASGVAVGALVHPTARMENGVTVDPGAVIGPRAEIGMGTVINAGAA
                     IGPNVRIGRQCTIGANVSITNSLIGDRVVVHPGCAIGQDGFGYVMGAAGHRKVLQVGR
                     VIVQDDVEIGAGTAIDRGANRDTVIGEGTKIDNLVQIGHNVRIGRHCIIVSQCGISGS
                     VVIEDRVILAGQVGLADNVTIGEGAIVGARSGVMSDVPAGEKWFGYPAMRGREFLRSM
                     MTLRHWVSENDAAGNGDPHDGPERRGLNDRRRRSR"
     gene            complement(1774..>2538)
                     /locus_tag="VM910_20390"
     CDS             complement(1774..>2538)
                     /locus_tag="VM910_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HVI64918.1"
                     /translation="GEASLAVKSAVANGPVLTSLVGYTLTHNTLDNNKNPTSGLLAEF
                     RQDFAGLGGDVRFVRTTADLYNYREVFPDIVGMLHLQGGQVAALGGGGLRMLDEFQMG
                     PNLVRGFAPAGIGPRDLTQFPFTGVLGDALGGSYYWGASFELQTPLYFLPKDAGIKVA
                     AFADAGSLWNYSGPTTFPATGEVISGSFCPTFGPGIPNAAPCPVDNAMHVRSSVGVGI
                     IWESPFGPLRFDYAFPLSKEPYDRVQQFRFGGGTKF"
BASE COUNT          457 a          847 c          781 g          453 t
ORIGIN      
        1 cggtgcgcgg gccgatcgtg gtatggccgg cgatgtgaac gtgggccaag agcctacaac
       61 cgtcgccgat tttgaccccg gggccgacga cgcaatacgg cccgatcgcc acgtcctgcc
      121 cgatcaccgc gcccggttcg atccgggcgg ttggatcgat tgcggccatc gccgacctca
      181 cgcaaccatc gcgccgactt cggcctcggc gaccgtgtgt ccggcgacct tcgcctcgcc
      241 gcgaaaccac cacatgttct ttttccgggc gatctttctg acgtggtatt cgatcgtgtc
      301 ccccggcacc accggcttgc ggaacttcgc cttgtcgatg gtgaggaaat agacctgctt
      361 cggatgatcg ccgcccatct gcgacagcac gcagatcgcg ccggccgtct gcgccatgcc
      421 ctcgatcagc aacacgccgg gaaacaccgg attgccgggg aaatgcccga cgaactgcgg
      481 ttcgttgacg gtgacgttct tgattccaat ggcggcttca tcaccccgca tgtcgatgat
      541 gcggtcgacc atcaaaaacg gatagcgatg cggtagcgct ttgaggatgg ctgcgatatc
      601 ggctgcctcg agcgtggtgg tcgccgcctt gttcatcggc ttcgccttcg ccgatcatta
      661 aggccgcgtc gctcgggtcc gtcgtgaggg tcgccgttgc cggcagcatc gttttcgctc
      721 acccaatggc gcaacgtcat catgctgcga agaaactcgc ggccgcgcat cgccggatag
      781 ccgaaccact tttcgcccgc cggcacgtcg gacatgaccc cgctcctggc tccgacaatc
      841 gcgccctcgc cgatcgttac gttgtccgcc agcccaacct gtccggccag gatgacgcga
      901 tcctcaatga cgacgctgcc ggagatgccg cattgcgaca cgatgatgca gtggcggccg
      961 atcctcacat tgtgtccgat ctgcaccaag ttgtcgatct tggtgccttc gccgatcacg
     1021 gtgtcgcggt tggcgccgcg atcgatggcg gtcccggcgc caatctcaac atcgtcctga
     1081 acaataaccc ggcctacctg cagaactttg cggtgaccgg ccgctcccat cacgtagccg
     1141 aagccgtcct gtccgatggc gcagccggga tgcacgacca cacgatcgcc gatcaagcta
     1201 ttggtgattg aaacgttggc accgatggtg cactggcggc cgatccgcac gttgggtcca
     1261 atcgcagcgc ccgcgttgat caccgtgccc attccgatct cggcgcgcgg gccgattacg
     1321 gccccaggat ccacggtcac gccgttttcc atgcgcgcgg tggggtggac tagagccccg
     1381 actgccacgc cgctcgcctc gaacaaggag gacggccgca gcgcatccgg aaacagggcc
     1441 cgcgtcacct cgacgaaagc gcgataaggc tcggcgacgc agagcacgct gacatgtgcg
     1501 ggagcgcggc cagcgtagcg ttccgtcgtc aaacacgcgc cggcaccgct cgtcgacaat
     1561 tggtgcgcgt atttcgcctt ttcgaggaat gcgagatcgc tgggggcggc ctgatcgagc
     1621 gctgcaatcc cggtgatgcg gcggtcaaga tcgctgccag gccgcggcgc ggcgcccgtg
     1681 agagcggcga tctcgcgcac gctgagcccg cgtccccgat tgaagaaaaa gggctcggtc
     1741 atgcccgtgc cgatcatggc cccgcaggcg gggtcagaat ttcgtcccgc cgccgaagcg
     1801 gaattgctgc acccggtcgt agggttcctt gctgagcggg aatgcataat cgaaccgcag
     1861 cggcccgaat ggtgattccc aaattattcc cacgcccacc gagctgcgca cgtgcatggc
     1921 gttgtccacc ggacacggcg ccgcgttggg gatgcccgga ccgaaggtcg gacagaaact
     1981 acccgagatg acctcgccgg tggcggggaa tgtcgtcggg ccgctataat tccagagcga
     2041 gccggcatcg gcgaaggcgg caaccttgat ccccgcatcc ttgggcagga agtagagcgg
     2101 cgtctgcaac tcgaaactgg cgccccaata gtacgagccc ccgagcgcat cccctaaaac
     2161 gccggtaaac ggaaactgcg tcagatcgcg tggtccaatt cccgccggtg caaatccgcg
     2221 caccagattg gggcccatct gaaactcgtc gagcatgcgc agcccgccgc cgcctagcgc
     2281 cgcgacctgc cccccttgca gatgcagcat gccgacaatg tcgggaaaca cttcgcgata
     2341 gttgtagagg tcggccgtcg tgcgcacgaa cctgacgtcg ccgccaaggc cggcaaaatc
     2401 ctgcctgaac tcggccaaca gtcccgaagt cggattcttg ttgttgtcga gcgtattgtg
     2461 cgtgagcgtg tagccgacca acgaggtgag aaccgggccg ttcgccacgg cgcttttcac
     2521 ggcgagtgac gcctcgcc
//
LOCUS       DATLJT010000883         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3504538,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000883 DATLJT010000000
VERSION     DATLJT010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3504538"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VM910_20395"
     CDS             complement(<1..1062)
                     /locus_tag="VM910_20395"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HVI64919.1"
                     /translation="MPIQVLMPALSPTMEKGNLAKWLKKEGETIKSGDVIAEIETDKA
                     TMEVEATDEGTLGKILIPEGTADVAVNTPIATILADGESTADLAKVTPPAKQEKAAES
                     APPAEAKSQAPASPAPAAPAAPAAIAAPDPEVPAGTEMVTMTIREALRDAMAEEMRRD
                     GDVFIMGEEVAEYQGAYKVTQGLLQEFGAKRVIDTPITEHGFAGVGVGAAMAGLKPIV
                     EFMTFNFAMQAMDQIINSAAKTLYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYSAWY
                     SQIPGMKVIAPFSAADYKGLLKAAIRDPNPVIFLENEVLYGHSGEVPKLDDYVIPIGK
                     ARVARVGSHVTIIAWSMGMS"
     gene            complement(1152..2174)
                     /gene="pdhA"
                     /locus_tag="VM910_20400"
     CDS             complement(1152..2174)
                     /gene="pdhA"
                     /locus_tag="VM910_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HVI64920.1"
                     /translation="MAAPKKTDTKEKGQDKGNGSPPEFTKEQELKALRDMLLIRRFEE
                     KAGQLYGMGAIGGFCHLYIGQEAVVVGMQMALKKGDQVITGYRDHGHMLACGMEAKGV
                     MAELTGRRGGYSKGKGGSMHMFSKAENFYGGHGIVGAQVSLGTGLAFANRYRGNDSVS
                     LAYFGDGAANQGQVYESFNMAELWKLPVVYVIENNRYAMGTSVKRSSAQTDFSKRGVS
                     FNIPGEQIDGMDVRAVKAAGDKAVAWCRAGNGPFILEMQTYRYRGHSMSDPAKYRTRE
                     EVDKIRHDQDPIEQVRNRLLAAKVAEQELKAIDAEVREIVNAAADFAQHDPEPDPSEL
                     YTDVYR"
     gene            complement(2336..2656)
                     /locus_tag="VM910_20405"
     CDS             complement(2336..2656)
                     /locus_tag="VM910_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVI64921.1"
                     /translation="MVSRARLKSFLTGLALYTMAAALVGYFGVNAYTGKYGLNARQEL
                     DQEIIALTSELSRLKRERAESEQRVSLLRSDRVDPDMLDERARFQLDYANPHDLVRMN
                     NPSR"
     gene            complement(2748..>3164)
                     /locus_tag="VM910_20410"
     CDS             complement(2748..>3164)
                     /locus_tag="VM910_20410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HVI64922.1"
                     /translation="FNQDLEAAPPADWLAFREKLQKSNGVLFVTPEYNRSIPGVLKNA
                     IDVGSRPYGKSSFLGKPTGIISNSPGPLGGVSAAKQLQNILPGISGPIMGQPETYLNA
                     IGDAFNDKGELVKEGVQKILQQYLEAFAAFVEKQNR"
BASE COUNT          565 a          965 c          953 g          681 t
ORIGIN      
        1 ggacatgccc atcgaccagg cgatgatcgt gacatggctg ccgacacgcg cgacgcgcgc
       61 cttgccgatc gggattacgt aatcgtcgag cttcggcacc tcgccgctat ggccgtacaa
      121 gacctcgttt tcgaggaaga tgaccggatt gggatcccgg atcgcggcct tcaacaggcc
      181 cttgtaatcg gccgccgaga acggtgcgat cactttcatt ccgggaatct gcgagtacca
      241 ggcggaatag tcctggctgt gctgcgcggc gacccgggcc gccgcgccgt tcggcccgcg
      301 aaacacgatg gagcaaccca tctgcccgcc ggacatatag agcgtcttgg cggccgaatt
      361 gatgatctgg tccatcgcct gcatcgcgaa gttgaaggtc atgaactcga cgatcggctt
      421 gagacccgcc attgcggcgc cgacgccgac gccggcgaaa ccgtgttcgg tgatcggcgt
      481 gtcgatcacg cgctttgcgc cgaactcctg cagcagtccc tgcgtaacct tataggcgcc
      541 ctgatattcc gcgacctcct cgcccatgat gaacacgtcg ccatcgcgcc gcatttcttc
      601 cgccatcgca tcgcgcagcg cttcgcggat ggtcatggtc accatctcgg tgccggccgg
      661 cacttccgga tcgggtgcgg caatcgcggc aggtgcggca ggtgccgcgg gcgcaggcga
      721 cgccggtgcc tgactctttg cttcggccgg tggcgcggat tcggcagcct tctcctgctt
      781 ggccggcgga gttacttttg caaggtcggc cgtgctttcc ccatcggcaa gaatggtggc
      841 aatcggcgtg ttgacggcga cgtcggcggt gccttcggga atcaggattt ttccgagcgt
      901 gccttcatct gttgcctcga cctccatcgt cgctttgtca gtttcaatct cggcgatgac
      961 gtcgccggac ttgatggtct cgccttcctt tttcagccat ttggcgaggt tgcccttctc
     1021 catggtgggc gacaacgcgg gcatcagcac ttgaatgggc atatcaactc ccgaaatcac
     1081 tctctcgttt cttttacgcg tagtctgatc acaatggatg gtccgccatt tgccgaatac
     1141 gcgccgccct tctagcgata gacgtcggtg taaagctcgg aaggatcggg ctcaggatcg
     1201 tgctgagcga aatcagccgc cgcattgacg atctcgcgca cctcggcgtc gatggccttc
     1261 aattcctgtt cggcgacctt cgctgcgagg aggcggttgc ggacctgctc gatcggatcc
     1321 tgatcgtggc gaatcttgtc gacctcctcg cgcgttcggt atttcgcggg gtccgacatc
     1381 gaatggccac gatagcgata ggtctgcatc tccagaatga acggcccgtt gccggctctg
     1441 caccaggcga ccgccttgtc gccggcggcc ttgacggcgc gcacgtccat gccatcgatc
     1501 tgctcgcccg ggatattgaa ggagacgccg cgcttggaga aatcggtctg tgccgaggag
     1561 cgcttcaccg aagtacccat ggcatagcgg ttgttctcga tgacatagac aaccggcagc
     1621 ttccagagtt cagccatgtt gaagctttcg tagacctgtc cctggtttgc ggcgccgtcg
     1681 ccgaaataag cgaggctcac ggagtcgttc ccgcgatatc ggttggcgaa agcgagcccg
     1741 gtaccaagcg acacctgggc gccgacgatg ccatggccgc catagaagtt ctccgccttg
     1801 gagaacatgt gcatggagcc gcccttgccc ttggagtagc cgccgcgacg gccggtcagt
     1861 tccgccatca cgcccttggc ctccatgccg caggccagca tgtgcccgtg gtcacggtat
     1921 cccgttatca cctgatcgcc ctttttcagg gccatttgca tgccgaccac gaccgcttcc
     1981 tggccgatgt aaaggtggca aaaaccgcca atcgccccca tgccgtagag ctggccggcc
     2041 ttctcctcga agcgccggat caggagcatg tcgcgcagcg ccttgagctc ctgctctttg
     2101 gtgaattcgg ggggagaacc gtttcccttg tcctgaccct tttccttggt gtcggttttc
     2161 ttgggtgcgg ccatggcaaa tccggcaatg agagagcgat gcgacctctc taacccaact
     2221 caaacggccg tggaaggcgt gcgtgatggg caaaaggctt tgatttgtcg cactgcaacg
     2281 cggcacgaaa ctttcgacac cgatttggaa gatttttgaa atgccggctg ttgatctacc
     2341 ggctcggatt gttcatccga acaagatcgt gcggattggc gtaatcgagc tgaaagcgcg
     2401 cccgctcgtc cagcatatcc ggatcgaccc ggtcggagcg aagcagcgag acgcgctgtt
     2461 cgctttcggc acgctcgcgc ttgagccgcg acagctctga cgtcagcgcg atgatctcct
     2521 ggtcgagctc ctgacgcgcg ttcaagccgt acttgccggt gtaagcgttg acgccgaaat
     2581 agccgacgag agccgccgcc atggtgtaga gggcaagccc ggtcaggaag gatttgagtc
     2641 gtgcgcggga aaccatgctc tattaccgcc gattgtgggc ggtcgtagca tgcgtccgcg
     2701 ccggttaacg aaaacctaaa gcacgaaggc gcccgccggg agcgaaatca tctgttctgc
     2761 ttttcgacaa aagcggcgaa ggcctcgaga tattgctgca ggattttctg cactccttcc
     2821 ttcacaagct cgcccttgtc gttgaaggca tccccgatcg cgttcagata ggtctcgggc
     2881 tgtcccatga tcgggcctga aataccgggc agaatgttct gcagttgctt tgcggcgctg
     2941 acgccaccga gcggccccgg cgaattcgag ataatgccgg tcggcttgcc gaggaacgag
     3001 ctcttgccat agggccgcga gccaacatcg attgcgtttt tcaggacgcc cgggatcgag
     3061 cggttgtatt cgggagtgac gaaaagcacg ccgttcgact tttgcagctt ttcgcgaaac
     3121 gcgagccagt cggctggtgg cgccgcttcc agatcctggt tgaa
//
LOCUS       DATLJT010000884         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_58380,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000884 DATLJT010000000
VERSION     DATLJT010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58380"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1182)
                     /locus_tag="VM910_20415"
     CDS             complement(16..1182)
                     /locus_tag="VM910_20415"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HVI64923.1"
                     /translation="MTIQQNTDHIQTTHIGSLPRPHNLLDLLKAKYAGQPYDESKLNS
                     VLAREVAECVRKQVECGIEIVTDGEYSKPGFFTYIRERFDGFESRPNQKLTIFQQEVS
                     AFPEYYAQYFKEAMMGGAILPIVPVVCTAPVKYKGEAKLQIDIANVKAAAKAAGVPDH
                     QLFLPATAPSGVGINEYYKSEEEYFHALAAELNKEYRAIVAAGILVQVDDPFLPDIFV
                     EPGLDDRQKKRKAEIYVEATNAALSGIPPERVRFHTCYGINEGPRIYESNLPEIIAYV
                     LRINAGSLSFEAANPRHEHEYHLFERVKVPEGKVLCPGVITHASNIVEHPELIAERLI
                     RFANLVGRENVMGGADCGFSSQALYRTEVHYSVVWEKFKALRQGADLASKQLWG"
     gene            complement(1282..1938)
                     /locus_tag="VM910_20420"
     CDS             complement(1282..1938)
                     /locus_tag="VM910_20420"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI64924.1"
                     /translation="MMVVPLPTRRLRPSPRRRAPEIIEAAARVFAQRGFHGATTQDIA
                     DVLGIRQASLYYYFPSKEGALELVCLQGVAGYFEVAKAIASGPGAAADKLARLVKSHL
                     APLADRSDFVRVFLNERQHLPSESRRRVGKWSRGLERVFEDVLKEGVRRGEFRPDLDT
                     RLAVLGILGMANAVANWYRKEEAAIERIGGEFARLVVDGVKKRPKLRSRIPGVRRSAR
                     "
BASE COUNT          384 a          657 c          632 g          426 t
ORIGIN      
        1 gatagatgag gcggttcagc cccagagctg tttgctggcg aggtcggcgc cctggcgcaa
       61 ggctttgaat ttctcccaca ccacgctgta gtgcacttcc gtgcgataca aagcctggga
      121 cgagaagccg cagtcggcgc cccccatcac gttctcgcgg cccaccaagt tggcaaagcg
      181 gatcagccgt tcggcgatca attccgggtg ctcgacaatg ttgctggcat gcgtgatgac
      241 accagggcat agcactttcc cttcgggcac cttcacccgc tcgaacaagt ggtattcgtg
      301 ttcgtggcgc ggattcgcag cctcgaagct gagcgaaccg gcgttgatcc tgagcacgta
      361 cgcgatgatc tcgggcagat tcgattcgta gatgcggggt ccctcgttga tgccgtagca
      421 agtatgaaag cggacccgct cgggcggaat gccgctgagc gccgcattgg tcgcttcgac
      481 gtaaatctcc gcctttcgct tcttctgcct gtcgtcaagc cctggctcga cgaagatgtc
      541 gggcaggaat ggatcgtcca cttgcactag aatgccggcg gctacgatcg cgcggtattc
      601 cttgttcagc tcggcggcga gcgcgtggaa atactcttcc tcgctcttgt agtattcgtt
      661 gatgccgacg cccgagggcg ccgtggccgg cagaaacagc tgatggtcag gcacgcctgc
      721 ggccttggca gcggccttca cattggcgat gtcgatctgc agttttgcct cgcccttata
      781 tttcaccggg gcggtgcaga cgacaggcac aatcggcaga atggcgccgc ccatcatcgc
      841 ttccttgaaa tactgggcgt aatattcggg aaaagccgag acctcttgct ggaaaatagt
      901 gagcttctga ttgggccgag actcgaaccc gtcgaagcgc tcgcggatgt aagtgaaaaa
      961 gcccggcttc gagtattcgc cgtcagttac gatctcaatc ccgcactcga cctgcttgcg
     1021 cacgcattca gcaacctccc gcgccaagac cgaattcagc ttactttcat cataaggctg
     1081 ccccgcgtat tttgctttca gcagatcgag cagattgtgc ggccgcggca agctgccgat
     1141 atgcgtagtc tggatgtgat cggtattttg ctggatggtc attggtgttc cacgagttcg
     1201 tcatgacctc ttgaggtttg accgtctgca agcgcgcgag cctgcggcgg aggtaccaga
     1261 attgggcctc accggacggc gtcagcgagc ggaccggcgc acccctggta tacgggagcg
     1321 cagtttcggc cgcttcttca caccgtcgac cacaagtcgc gcgaactcgc cgccgatgcg
     1381 ctcgatcgcc gcctcttcct tgcggtacca gttcgccacc gcgttggcca tgccgaggat
     1441 gccgagcacg gccaaacgcg tatcgagatc ggggcgaaac tcgccacgcc gcaccccttc
     1501 cttgaggacg tcctcgaaca cgcgctcgag cccgcgcgac cattttccga cccgccggcg
     1561 gctttcgctc ggcaggtgtt ggcgctcgtt caagaagacc cggacaaaat cgctgcggtc
     1621 ggccagcggc gcgaggtgcg atttgaccag gcgcgccagc ttgtcggcgg cggcgcccgg
     1681 tcccgaagca atcgccttgg cgacctcgaa gtatccggcc acgccctgga gacaaacgag
     1741 ttcgagcgcc ccctccttgg acgggaaata gtagtacagg ctcgcctgcc ggatgccgag
     1801 cacatcggcg atgtcttggg tggtcgcacc gtggaagccg cgctgcgcga agacgcgcgc
     1861 agcggcctcg atgatctcgg gtgcccgccg ccgtgggctt ggccgcaagc gccgtgtcgg
     1921 cagcggcacg accatcatga cgtctcccca tccagccagg acggctccgg agttgtgttg
     1981 tgccacgtcg gcagattaaa ttctacctat tgatctatag acaaccggat acgagttgcg
     2041 aatgaccatt cgccgagatg gccgcccccc gtttcgcgcc gatcacatcg gcagcctgc
//
LOCUS       DATLJT010000885         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_975566,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000885 DATLJT010000000
VERSION     DATLJT010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975566"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VM910_20425"
     CDS             complement(<1..117)
                     /locus_tag="VM910_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVI64925.1"
                     /translation="MICFCSERFPWRRIWQNPSELLLPGLTDMATRALLYRRP"
     gene            complement(357..1685)
                     /gene="phaZ"
                     /locus_tag="VM910_20430"
     CDS             complement(357..1685)
                     /gene="phaZ"
                     /locus_tag="VM910_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HVI64926.1"
                     /translation="MPIGEFGGAPPLVAQGSPALTAPLYWMYEMAHASLNPARAVTDA
                     TKILFQNPLNPWSHTELGKSVAAACELFERTTRRYGKPEWGIHETEVNGIHAAVEIKS
                     IWERPFCQLLYFDRKLTRPLRHPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYITD
                     WSDARMVPLSEGRFDLDDYIDYVIEMLHVLGGNMHIIAVCQPSVPVLAAVSLMEAARD
                     PFTPISMTLMGGPIDTRRNPTAVNKLAEERGMEWFGRHVITKVPFPHPGVGRAVYPGF
                     LQLNGFISMNLDRHMDAHKALFRNLVKGDGDLVDKHRDFYDEYLAVMDLAAEYYLQTV
                     ETVFVRHALPKGEMTHRGKPVDPTKIARVALMTVEGEHDDISGLGQTEATHSLCTSIP
                     NHRRIHYVQKGVGHYGVFNGSRFRSEIVPRINDFMMSAMNPKARLAVAAE"
     gene            complement(1808..2626)
                     /locus_tag="VM910_20435"
     CDS             complement(1808..2626)
                     /locus_tag="VM910_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI64927.1"
                     /translation="MSELVLHRGISLNRIGAMILRYWYLLLSSWPRLLELTYWPVLQV
                     ITWGFLQTYIAQNANFFLRAGGTLIGAVILWDILFRGQLGFSISFLEEMWARNLGNLM
                     MSPLKPIEFLLALMAMSVIRLAIGLIPMTLLALVLFHFNFYSLGLPLIAFFCNLIFTS
                     WAVGIFVSGLVLRNGLGAESIVWTLMFALMPLACVYYPVQVLPHWLQYVAWSLPPTYV
                     FEGMRALLIDQVFRADLMLVSLLINAGLLVASFGAFLALLKSARRHGSLLSGGE"
     gene            complement(2623..>3111)
                     /locus_tag="VM910_20440"
     CDS             complement(2623..>3111)
                     /locus_tag="VM910_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI64928.1"
                     /translation="FGRLYAVADLRARIDELAHDLDLTEFLDRANGKLSAGQKTRVAL
                     AKALINQPELLLLDEPTASLDPDTADWVREHIETYRKRHEATILLASHNMFEVERLCD
                     RVIILKRGRIEEDDSPARIMARYSRDTLEQVFVDVARGRAQDAPSPLPVPQESVSPEA
                     MS"
BASE COUNT          662 a          938 c          972 g          539 t
ORIGIN      
        1 agggcgccga taaaggagcg cgcgagtggc catgtccgta agtccgggga gaagcagttc
       61 gctcggattc tgccatatcc gccgccaggg aaatcgctcg ctgcaaaaac aaatcatttg
      121 cccaatatac aggggtcggc cgacctccgg gccctacaga atgggcccgg aaccggcctt
      181 ttcgggggga accgggggtt caaatggcct cgaaggactg acgtcacaat cattacttcg
      241 gtagcgtcga atctccccct tcggagggcg tcgcaaaggg gtccaaaacc cgcgcccgcg
      301 accctattcg ggcgccaatc gcacgaaaac gccgcctgaa cgcgtctgtt cgcggcctat
      361 tcggcagcga ccgcgagccg agccttcgga ttcatcgccg acatcatgaa atcgttgatg
      421 cgcggcacga tctccgagcg aaaacgggaa ccgttgaaca cgccgtaatg cccgacaccc
      481 ttctgcacgt aatgaatgcg gcgatgattc ggaattgacg tgcatagcga atgcgttgcc
      541 tcggtctggc cgagcccgga gatgtcgtcg tgctcgcctt ccaccgtcat caacgcgacg
      601 cgtgcgattt tcgtcgggtc gaccggcttg ccgcgatggg tcatctcgcc cttgggcaac
      661 gcgtgccgca cgaacacggt ctcgaccgtc tgcagataat attcggccgc gagatccatc
      721 accgcgagat attcgtcata gaagtcgcga tgcttgtcga ccagatcgcc gtcgcccttc
      781 accaggttgc ggaacaaggc cttgtgggcg tccatgtggc ggtcgagatt catgctgatg
      841 aagccgttga gctgcagaaa gcccggatag acggcgcgcc cgacgccggg atgcgggaac
      901 ggcactttgg tgatgacatg tctgccgaac cactccatac cgcgctcttc ggcgagtttg
      961 ttgaccgcag tcggattgcg acgcgtatcg atcggcccgc ccatcagggt catcgagatc
     1021 ggcgtgaaag gatcgcgagc ggcttccatc agggaaaccg ccgccagcac cggcaccgat
     1081 ggctggcaca ccgcgatgat atgcatgttg ccgcccagca cgtgcaacat ctcgatgaca
     1141 tagtcgatat agtcgtcgag atcgaagcgg ccttcggaaa gcgggaccat ccgcgcgtcc
     1201 gaccagtcgg tgatgtagac ctcatgcgtc ggcaagaaag cctcgacggt gccgcggagc
     1261 agcgtcgcat agtgccctga catcggcgcc acgatcagca cgcgcggttg cgggtgacgc
     1321 aacgggcgcg tcaatttgcg atcgaagtag agcaactggc agaacggccg ctcccagata
     1381 ctcttgattt cgacggcggc atggatgccg ttgacttcgg tctcgtggat gccccattcc
     1441 ggcttgccgt agcggcgcgt tgtgcgttcg aacaactcgc aggccgcggc aactgacttg
     1501 ccgagttccg tgtgcgacca ggggttcagc gggttctgga acaggatctt ggtcgcatcc
     1561 gtgacggcgc gcgccggatt gagcgatgcg tgcgccattt cgtacatcca atagagcggc
     1621 gccgttagtg ccggactacc ttgggccacc agtggcggtg cgccgccaaa ctcaccaatc
     1681 ggcatgattc tcagacccct gtttgcgccg cagcatactg gcgaatgcgt aatattgcgt
     1741 caacgtgaag atcggcatga ttccccgact tcaccaccaa agtcccgtaa atccacggga
     1801 aagtgaccta ttcgccaccc gaaagcagcg atccgtgacg gcgggcgctc ttcaaaaggg
     1861 caagaaatgc cccaaacgaa gccaccagca ggcccgcgtt gatgagcagg gacaccagca
     1921 tcaggtcggc gcgaaacacc tgatcgatca gaagggcgcg cattccctcg aaaacatagg
     1981 tcggcggcag cgaccaggcc acatattgca gccaatgcgg cagcacctgg acgggataat
     2041 agacgcaggc aagcggcatc agcgcaaaca tcagcgtcca gacgatgctt tcggcgccaa
     2101 gcccgttgcg cagcacgagt ccggacacga agattccgac cgcccagctc gtgaagatca
     2161 gattgcagaa gaaggcgatc agcggcaggc cgaggctgta aaaattgaag tgaaacagaa
     2221 cgagcgcgag cagcgtcatc ggtatgaggc cgatcgcgag ccggatcaca ctcatcgcca
     2281 tgagcgcaag cagaaactcg atcggcttga gtgggctcat catcaggttg ccgaggttgc
     2341 gcgcccacat ctcctccaga aaggagatgg aaaagccgag ctggccgcgg aacaggatat
     2401 cccacaggat caccgcgccg atcagcgtac cgccggcgcg aaggaagaag ttggcatttt
     2461 gcgcgatata ggtttgcagg aacccccagg tgatgacctg cagcaccggc caatatgtca
     2521 gttccagaag ccgcggccag gacgacagca acaggtacca gtagcgcagg atcatcgcgc
     2581 cgatgcggtt gagcgagatg ccgcgatgca ggacgagctc gctcatgaca tcgcctcggg
     2641 agagacgctc tcctgcggca cgggcagtgg cgagggggca tcctgggcgc ggccgcgcgc
     2701 cacgtcgacg aacacctgct ccagcgtgtc gcggctgtaa cgcgccatga tgcgcgcagg
     2761 actgtcatcc tcctcgatgc ggccgcgctt caggatgatg acgcggtcgc agagccgctc
     2821 gacttcgaac atgttgtgcg aggcgagcag aatggtggcc tcatgtcgct tgcgataggt
     2881 ctcgatgtgc tccctcaccc agtcggcggt gtcgggatcg agcgaggcgg tcggctcgtc
     2941 gagcagcagc agctcgggct ggttgatcaa cgccttggca agcgcgacac gcgtcttctg
     3001 cccggccgac agcttgccgt tggcgcggtc gaggaactcg gtgagatcga gatcgtgcgc
     3061 gagttcgtcg atgcgcgcgc gcagatctgc aaccgcgtag aggcgaccga a
//
LOCUS       DATLJT010000886         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3963728,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000886 DATLJT010000000
VERSION     DATLJT010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3963728"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VM910_20445"
     CDS             complement(<1..669)
                     /locus_tag="VM910_20445"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HVI64929.1"
                     /translation="MSALRTIFCTCGAIFSLANTASAQVPAAPVQPTVPGEREFGQPV
                     LDSGIFAHVILNENEGRWNGTNTEYRWEGQGWVGTDYDRLWIKSEGTVSNGAVDDGQD
                     QFLYSRAITTYFDLQGGLRSDIDSRPTRNWAALGIQGLAPYFFDLELTGYASDQGHLA
                     AKLEGSYDLLITQRLILQPQLELNLYSKADPARLTGAGFSDIDTGLRLRYEFSREFAP
                     YIGVV"
     gene            complement(666..983)
                     /locus_tag="VM910_20450"
     CDS             complement(666..983)
                     /locus_tag="VM910_20450"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI64930.1"
                     /translation="MERFIWGFDGKKFSEAEPIVLQRGERVRFVLINDSMMEHPIHLH
                     GLWSELENGHGAYRPYKHTINVKPGEKLSYLVTADVPGRWAYHCHLLYHMELGMFREV
                     RVS"
     gene            complement(985..>2181)
                     /locus_tag="VM910_20455"
     CDS             complement(985..>2181)
                     /locus_tag="VM910_20455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HVI64931.1"
                     /translation="PINITGRQRTATAVNGSVPAPILRWREGDTVTLAVTNRLSEPSS
                     IHWHGILVPNPMDGVPGLTFAGIPPGETFVYRFPVHQSGTYWYHSHSTFQEQTGLYGP
                     LVIDPKGGYAQPFDRDYVVMLSDWTDTSPETIVSNLKFQSDYYNFQQRTVGTFFDDVA
                     KNGLAATVSDRLMWGQMRMSPTDILDVSGATYTYLLNGQPPARNWTALFRPGERVRLR
                     FINGSTMSIFDVRVDGLPMSIVQADGNEVVPVMVDEFRIGVAETYDVIVQPTADRAYT
                     IFVQSEDRTGYARETLAPRAGMEAAIPPMDPRPLRTMTDYGDGYAARFYVGHVQYGGR
                     RRGEETRGCCRCRQCRRDADRAAKPSRRRVSPRPARTHLRRLACDPARQRSSTPLARH
                     HPPPDR"
BASE COUNT          416 a          645 c          700 g          420 t
ORIGIN      
        1 gacaacgcca atatacggcg cgaactcgcg gctgaattcg tagcgcagcc gcagccccgt
       61 atcgatatcg gaaaatccgg cgccggtcag ccgggcggga tcggccttgc tgtacagatt
      121 gagctctaac tgcggctgca gtatcagccg ctgcgtgatg agcaggtcgt aggaaccttc
      181 cagcttcgcg gccagatggc cctggtcgct cgcgtagccg gtcagttcaa ggtcgaaaaa
      241 gtaaggtgcc aggccttgga tgccaagggc cgcccaatta cgggtcggac gtgaatcgat
      301 atcgctgcgc agcccgcctt gcagatcgaa ataggtcgta atggcgcggc tgtacaggaa
      361 ctggtcctgg ccatcgtcga cagcgccgtt gctcaccgtg ccttccgact tgatccacag
      421 cctgtcgtaa tcggtgccga cccacccctg cccctcccag cggtactcgg tgttggtgcc
      481 attccaccgg ccctcattct cattgagaat gacgtgggca aagatcccgc tatccaagac
      541 cggctgcccg aattcgcgct ctccgggcac tgtcggttga acgggtgccg cgggcacttg
      601 cgccgaggcg gtattggcga gggagaagat ggcgccgcag gtgcagaaaa tggtccggag
      661 ggcgctcatg agacgcgcac ctcgcggaac atgcccaact ccatgtggta gaggaggtgg
      721 cagtggtatg cccagcggcc gggtacgtcg gcagtgacta gataactgag cttctcccca
      781 ggcttgacgt tgattgtatg cttgtatggc cgatacgcgc cgtgcccgtt ttcgagttcg
      841 ctccataacc cgtgcagatg gatcgggtgc tccatcatcg agtcgttgat caggacaaaa
      901 cgcacccgct cgccgcgctg caggacgatg ggctcggctt cggagaactt cttgccgtcg
      961 aagccccaga taaagcgctc catattaccg gtcaggtgga gggtgatgtc gcgcgagggg
     1021 ggtcgaggat cgttgccggg ccggatcgca cgcaagtcgg cgtaggtgag tacgcgccgg
     1081 tctgggggaa acccttcgcc ggctcggttt agccgctcgg tcggcatctc ggcgacattg
     1141 tcgacaccga cagcacccgc gagtttcttc gcctcggcgc ctgcccccat actggacatg
     1201 tccgacatag aaccgggctg catacccatc cccatagtca gtcattgtgc gcagcggacg
     1261 gggatccatc ggcggaatgg ccgcctccat tcccgcacgc ggcgcaagtg tctcgcgggc
     1321 atatccggta cgatcctccg actgaacaaa gatcgtgtag gcgcggtcgg cggtgggctg
     1381 cacaattacg tcataggtct cggcgacgcc gatgcggaac tcgtcgacca tgaccggaac
     1441 gacctcattg ccgtcggcct gaacgatgct catcggcagg ccgtcgacac gcacatcgaa
     1501 gatgctcatc gtggagccgt taatgaagcg cagccgcacc cgttcaccgg gccgaaacaa
     1561 tgctgtccaa ttcctcgcgg gcggctggcc gttgagcagg taggtgtagg tggcgccgct
     1621 gacatcgagg atgtcggtcg ggctcatgcg catctgaccc cacatcagac ggtcggaaac
     1681 cgtcgccgca aggccgttct tcgcgacgtc gtcaaagaat gttcccaccg tgcgctgctg
     1741 gaaattgtag taatcgcttt ggaatttaag gttgctgacg atggtctcag ggctggtgtc
     1801 ggtccaatcc gacagcataa cgacgtaatc gcgatcgaac ggctgcgcgt agccgccctt
     1861 gggatcgatg acgagcgggc cgtagaggcc ggtttgctcc tggaacgtcg aatggctgtg
     1921 gtaccaataa gtcccgctct ggtggaccgg gaaacggtag acaaaggtct cgccgggggg
     1981 aatgccggcg aaagtcagac ctggaacgcc gtccatcgga ttgggcacga gtattccgtg
     2041 ccagtgaatc gaacttggct cggagagccg attggtgacg gccagcgtga ccgtgtcgcc
     2101 ttcgcgccag cgcaggatcg gcgcgggaac cgagccattg actgcggtcg cagtccgttg
     2161 acggccggtg atgttgatcg g
//
LOCUS       DATLJT010000887         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1896668,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000887 DATLJT010000000
VERSION     DATLJT010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896668"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VM910_20460"
     CDS             complement(<1..1122)
                     /locus_tag="VM910_20460"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVI64932.1"
                     /translation="MASRANQPLLLGCAVVCAAAFACSGIAPIRAAEDPAPVTVASAP
                     IASDARLAGDTKFTRFVLDLDKPIQFRAFALDDPYRVVVDTSQVSFRLPPGTGATGRG
                     LVKAFRYGMVMPGGSRIVFDLAGPARIANSTMLEAANGQPPRLVLELEEVDRATFVEA
                     LRAENRPQTKPALSESTAAVGVPQTVPAVKPGAPGDTRPVVVIDPGHGGVDNGTQASG
                     EIEKNLVLGFGLALRDRLEKSGKYRVVMTRTDDTFIALDDRVRFARNQKAALFVSIHA
                     DYLPRSEGDVQGATIYTLSDKASDAEAERLAETENRADAIGGVDLGEEPTDVADILID
                     LAQRETRTFSNRFARLLMGEMKTTVRMHKRPLKSAGFRVL"
     gene            1825..>3468
                     /locus_tag="VM910_20465"
     CDS             1825..>3468
                     /locus_tag="VM910_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HVI64933.1"
                     /translation="MPNKMLIDATHPEETRVVVVRGNRVEEFDFETAQRKQLRGNIYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPVADRQALIEAEERAHREADE
                     EDEQHSGRRRSRHRNHRRRGSGERIRSEIVEDAASDASAGAIASERGAEHHGGEDELP
                     EHGYDETIPDHLAVPEPIAAESGSQPDAPGEGPHDEFEEQPEHEPGRDIYERAAEAAA
                     PQDPRSETPVTAAVEASVAAATEQPFAAEDSEGRADDARAEEEQTQENFAHAEDAPHD
                     EAVAEAHAAPIEGEIIAPVIGDEHDEDEAEDAEEEVVESVGGEDVLEEVPERPYRPRR
                     QYKIQEVIKRRQVMLVQVVKEERGNKGAALTTYLSLAGRYAVLMPNTARGGGISRKIT
                     SAQDRSRLKEVVQDLDVPEGMGIILRTAGASRTKPEIKRDFEYLIRMWETVRDTTLKS
                     QAPTLVYEEGSLIKRSLRDLYNKEIDEILVAGESGYREAHDFMKMLMPSNVRAVKQYR
                     DGQPLFSRMGVESQLDAMFSPTVQLKSGGYIVINQTEALV"
BASE COUNT          663 a         1124 c         1106 g          575 t
ORIGIN      
        1 cagcacgcga aagcccgccg acttcagggg acgtttgtgc attcgcaccg tggtcttcat
       61 ctcacccatc agcagacggg cgaagcggtt tgagaaggtg cgggtttccc gctgtgccag
      121 atcgatcagg atgtcggcga cgtcggtcgg ctcttcccct aaatcgaccc cgccgatcgc
      181 atccgcccgg ttttccgttt cagccagccg ttcggcttcg gcgtcggagg ccttgtcgga
      241 aagcgtgtag atggtagcgc cctgcacgtc gccttcgctg cggggcaggt agtcggcgtg
      301 gatcgagaca aacagggcag ccttctggtt gcgcgcaaat cggacgcgat cgtcgagcgc
      361 gatgaacgta tcgtcggtgc gcgtcatgac cacgcgatat ttgccgctct tctccaggcg
      421 gtcgcggaga gccagaccga agccgagcac caggttcttt tcgatttcgc cgctcgcttg
      481 cgtgccgtta tcgaccccgc catggccggg atcgatcacg accaccgggc gcgtgtctcc
      541 cggcgcgccc ggctttacgg ccggcacggt ctgcggcacc ccgactgcag cggtcgactc
      601 cgatagcgcc ggcttcgtct gcgggcggtt ttccgctcga agcgcttcaa cgaaggtcgc
      661 gcggtcgacc tcttccagct ccagcaccaa tcgcggcggc tggccgttgg cagcttccag
      721 catggtggaa ttggcaattc tggcgggtcc cgcgagatcg aacacgatgc gtgaaccgcc
      781 cggcatcacc atcccgtacc ggaaggcctt caccaatccg cgccccgtag ccccggtgcc
      841 aggcgggagg cgaaagctca cctgggaggt gtcgacgacc acccggtaag ggtcatcaag
      901 ggcaaaggcg cgaaactgga tgggcttatc gaggtcgagg acaaagcgcg tgaacttggt
      961 gtcgcccgca agtcgcgcat cggaggcgat cggagcgctc gccacagtga ccggcgccgg
     1021 gtcctcagcc gcgcgaatcg gcgcgatgcc ggagcaggca aatgctgcag cgcacaccac
     1081 ggcgcatccg agcaaaagag gttgatttgc gcggctcgcc accgattcct tgcctccgaa
     1141 gcccagcttt taccgcatta gaaccacagg gttaatcgac ctttattgtg taaatcgtga
     1201 ataagggtga ctgtggcggg gcggagacgc ttcccttgca cggggcgccc aatccacgta
     1261 tgtactggag tgctgatggc cattactccc ggttgtgtcg cattcagcct cccggtgcag
     1321 cgccggaacc cgccgaattc gggcgattcc agcgcttttc cgtccgtcaa agagccagcg
     1381 cgagatgcgc ggccggcaag aggggcggtt cgagcccggg tggcagtccc aggttgtttt
     1441 acgttccagg gacggctttt gagaggcggc acagtccggt gttcggatcc cgcaggggtt
     1501 cgtatcaaat ttcagccaag cgctccggcg cgaaacctgg ccttcagcga gcgactaggg
     1561 cggcccctgc cgccaaactg ttccagacgg gccgacgcgt gatgcgccag actgtgttgc
     1621 cgaccgaact tagcggaaaa accgcgccga tgcggagctc aagctccgtc tttcgccgct
     1681 tcccgctccg atcgcgttcg gctcggcgtt cccggttgcg tttcggcctt cccctaaccc
     1741 ccgaatcagg tggaccgtcg cgttatccag cggcgcactt cgcgctcacg gccatacgcg
     1801 ccagccgcct ccaagagttc caaaatgccg aacaaaatgt tgatcgatgc cacccacccg
     1861 gaagagaccc gggttgtcgt ggtccgtggg aatcgcgtcg aagaatttga tttcgagacc
     1921 gcgcagcgca agcaattgcg cggcaacatc tacctcgcca aagtcacgcg ggtagagccc
     1981 tcactgcagg cggcctttgt cgaatatggc ggcaaccgcc atggcttcct cgcctttagc
     2041 gaaatccatc ccgactatta tcaaattccg gtcgccgacc gccaggcgct aatcgaggcc
     2101 gaggaacgcg cccatcgcga ggccgatgag gaagacgagc agcattccgg ccgccgtcgc
     2161 tcgcgtcacc gtaaccaccg ccgccgcggc agcggcgagc gtatccggag cgagatcgtc
     2221 gaagacgccg cctccgacgc atccgccggg gcgatcgcat cggagcgcgg cgccgaacat
     2281 cacggcggcg aggacgaact gcccgagcac ggttacgacg agaccattcc tgaccatctg
     2341 gcggtgcccg aaccgatcgc ggccgaatcc gggtctcaac ctgacgcccc tggagagggg
     2401 cctcacgatg agttcgagga gcagcctgaa cacgagccgg ggcgcgacat ctatgagcgc
     2461 gccgccgaag ccgcagcccc gcaggatccg aggtcggaga cgccggtgac ggcagcggtc
     2521 gaagcctccg tcgcggcggc caccgagcaa ccgtttgcgg cagaggacag cgaaggccgc
     2581 gctgacgacg cacgcgccga agaggagcag acgcaggaga acttcgctca cgccgaggac
     2641 gccccccacg atgaggccgt ggctgaggcg catgcagccc cgatcgaggg cgaaatcatc
     2701 gcacccgtga tcggcgacga gcatgacgaa gacgaagccg aagacgccga agaggaagtc
     2761 gtcgagtccg tcggtggcga ggacgtgctt gaggaagtgc cggaacgccc ctatcggccg
     2821 cgccgccaat acaagattca agaggtcatc aagcgccgcc aggtcatgct ggtgcaggtg
     2881 gtcaaggaag agcgcggcaa caagggcgcc gcgctcacaa cctatctgtc gctggccggc
     2941 cgctatgcgg tcttgatgcc caataccgcc cgcggcggcg gcatcagccg caagatcact
     3001 tccgcacagg accgctcgcg cctcaaggag gtggtgcagg atctcgatgt ccccgagggc
     3061 atgggcatca tcctgcgcac cgccggcgcc tcgcgcacca agccggagat caagcgcgac
     3121 ttcgaatatc tgatccgcat gtgggagacc gtgcgcgaca ccacgttgaa gtcgcaggca
     3181 cccaccctgg tctacgaaga gggttcgctg atcaagcggt cgttgcgtga cctctacaac
     3241 aaggagatcg acgagatcct ggtcgccggc gagtccggct accgggaagc gcacgatttt
     3301 atgaagatgc tgatgccctc gaacgtccgg gcggtgaagc aatatcgcga cggccagccg
     3361 ctgttctcgc gcatgggcgt ggaaagccag ctcgacgcga tgttctcccc gaccgtgcag
     3421 ctcaaatccg gcggctacat cgtgatcaac cagacggagg cgctggtt
//
LOCUS       DATLJT010000888         1715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4193934,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000888 DATLJT010000000
VERSION     DATLJT010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4193934"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VM910_20470"
     CDS             <1..445
                     /locus_tag="VM910_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI64934.1"
                     /translation="YRHILIPTDGSELARSAVQHGLSLAKAVGAKVTALTVEASFDVY
                     TVPASRVYEMSGAFAEHAERAKAHAEKILNGVAEEARQAGVACETVQIEEDHPYEAII
                     ETAKQRGCDLIVMASHGRSGIAAIVLGSVTTKVLTHTKIPVLVCH"
     gene            510..725
                     /locus_tag="VM910_20475"
     CDS             510..725
                     /locus_tag="VM910_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HVI64935.1"
                     /translation="MPFRSNSDLPASVQTHLPPHAQDIYREAFNHAFAAHMSDPRREE
                     RAHRIAWTAVKRSYVKVGDSWIGRGGN"
     gene            complement(837..1643)
                     /locus_tag="VM910_20480"
     CDS             complement(837..1643)
                     /locus_tag="VM910_20480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI64936.1"
                     /translation="MSFSTMMVHVDIEHDSERRIELAISLADRFQATLIGVAGYALWP
                     AFMAGDVGLTESNQYDLQKATARFDERGKTFRGQGRSLKQIEWRSALEPPGGLLLREA
                     RAADLLIIGRRRSENDADPGVIVLRAGRPVLLVPDTIAALPLRRVVVAWKDTRECRRA
                     VRDALPFLQQAKEVLLVEVGEDESRSQAKKNLADVAAYLVRHRVIVAAEVWRRPRGPA
                     AAELLHVVKEEKADLIVAGGYGHSRLGEWIFGGVTRELLAASPVCCMLSH"
BASE COUNT          328 a          547 c          545 g          295 t
ORIGIN      
        1 gtatcggcat attttgatcc cgaccgacgg ttcggagctt gcccgcagcg cggtgcagca
       61 tgggttatcg cttgccaagg cggtcggagc aaaagtcacc gcattgacgg tggaggcatc
      121 gttcgatgtc tacacggttc ctgcttcgag ggtctatgaa atgtcgggag cttttgccga
      181 gcatgccgag cgcgccaaag cgcacgcgga aaagatactg aatggcgtcg ccgaagaagc
      241 gcgccaggct ggcgtagcct gcgaaaccgt gcaaatcgaa gaggaccatc cttacgaagc
      301 catcatcgag accgccaagc agcgaggctg cgatctcatc gtcatggcct cgcacggcag
      361 gagcgggatt gcggcgatcg tgctcggcag cgtgacgacg aaggtgctga cgcacacgaa
      421 aattccggtt ctggtgtgcc actgatcgct tacggcttga aatcaggcac ctgccctcgc
      481 gcttggatcg ggctgcccgg gtgacggcca tgccattccg ttccaacagc gatctgcctg
      541 cgtcggttca gacacacctc ccaccgcacg cccaggacat ctaccgcgag gccttcaatc
      601 atgccttcgc ggcgcacatg agtgatccgc gccgggagga acgcgcgcac cgcatcgctt
      661 ggacggcggt caagcgctcg tacgtgaagg tcggcgacag ctggatcggc cgcggcggaa
      721 actgacctat cgcttcgcca ggcctgagtg catcagatcg tcgtcagaag ggccgtcgaa
      781 gacgcggaga cgatccaacg gtcggcgggt agccacagcc gttcatgacg cgaaggtcaa
      841 tgggacaaca tgcaacaaac tgggctcgcc gcgaggagct cgcgcgtgac gccgccgaaa
      901 atccattcac ccaatcggct gtggccatag ccgccggcca cgattagatc tgctttttcc
      961 tccttcacaa cgtgcaggag ctcggcggcg gccgggcccc gcggtcggcg ccagacttcg
     1021 gccgccacga tcacgcggtg ccggacgagg taggcggcga catcggcgag attcttcttg
     1081 gcctgcgacc gcgattcgtc ctcgccgact tcgacgagca gaacctcttt cgcctgctgc
     1141 aagaatggga gcgcgtcccg caccgcgcgt cggcattcac gcgtatcctt ccaagcgacg
     1201 accacgcggc gaagcggcag cgcagcgatg gtgtcgggca ccagcagaac gggccggccg
     1261 gcccgaagca cgataacccc ggggtcggcg tcgttctcgc tgcgcctgcg accgatgatg
     1321 aggagatcgg ccgcccgcgc ttcgcgcaag agaagcccgc ccggaggttc aagggcgctg
     1381 cgccactcga tctgcttgag cgagcggccc tggccgcgaa acgtcttgcc cctctcatcg
     1441 aaacgcgccg tcgccttttg aaggtcgtat tggttggatt cggtcagccc gacatctccg
     1501 gccatgaagg ccggccacag tgcgtacccc gcaacgccga taagggtggc ttggaagcgg
     1561 tcggccaggc tgatcgccag ttcaatcctc cgctccgaat cgtgctcgat gtcgacgtgg
     1621 accatcattg ttgagaatga catcgcgggc tccggcttgc ggccatcaag gaaacgaacc
     1681 gaccgacctt tccgatcgcg cggtggtcac ggtgg
//
LOCUS       DATLJT010000889         4457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3275130,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000889 DATLJT010000000
VERSION     DATLJT010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275130"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VM910_20485"
     CDS             <1..271
                     /locus_tag="VM910_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HVI64937.1"
                     /translation="ELADEQDEFYSEEDADTVAQIKDLIETRVRPAVANDGGDITFRG
                     FKDGVVYLNMKGACSGCPSSTATLQHGIQNLLRHFVPDVVEVRPM"
     gene            470..1165
                     /gene="tsaB"
                     /locus_tag="VM910_20490"
     CDS             470..1165
                     /gene="tsaB"
                     /locus_tag="VM910_20490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVI64938.1"
                     /translation="MLILAIDTALDACAAAILDTGTSKLIAMESLSMKRGHAEALMPL
                     IARVMKDAALPFVALDRIAVTTGPGSFTGLRVGLSAARGIALAAGKPAVGLTTLSAYA
                     APIVSEGGEEPVISAIDARHDQVYFQAVSGNGSSLVRPRVGKIEEALAASRFGAPHLV
                     GNAAKILADRWPKDAPQPVQIDAQAAPDISWVAWLGAAVSPDTAPARPFYLRAPDAKP
                     SQDVTPPQPATSS"
     gene            1162..1641
                     /locus_tag="VM910_20495"
     CDS             1162..1641
                     /locus_tag="VM910_20495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64939.1"
                     /translation="MSWLSELLGTGTPVIEPATIRDAPRLAQLHGTSFHRGWGESEFE
                     RMLAERNTLVQRLRLRRKIIGFAVSRMGADEAEILSIAIDAGHRGKGFSRNLLLTHLG
                     HLSGYGVRSIFLEVEENNHPARRLYQWAGFAVVGRRERYYQQDGEQLNALLMRRDLS"
     gene            1849..2298
                     /locus_tag="VM910_20500"
     CDS             1849..2298
                     /locus_tag="VM910_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HVI64940.1"
                     /translation="MTVLKSTSAPKPSGIEARCAATGMRMTEQRRVIARVLADAVDHP
                     DVEELYRRCVAVDDKISISTVYRTVKLFEDAGIIERHDFREGRARYEQMRDSHHDHLI
                     NLRDGKVIEFTSEEIEKLQAEIARKLGYKLVDHRLELYCVPLDDGGT"
     gene            2315..3004
                     /locus_tag="VM910_20505"
     CDS             2315..3004
                     /locus_tag="VM910_20505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI64941.1"
                     /translation="MTIDLIIFDCDGVLVDSEIISCRAHAATLTRYGYPITPEQVFQR
                     FLGRSVRQANLEVEAELGCALPDDYHTELQDEVYRCFEAELAPIPQIHQTLDQIELPV
                     CVASSGSHQRMRISLGRTGLYERFAPHIFSASEVANGKPAPDLFLLAAKRMATPPARC
                     LVIEDSVPGIAGARSAGMNVLGFHGGSHCGHDHAATLRAAGAHGTFGDMRQLPGLIAQ
                     KAANPDRGEGA"
     gene            3102..>4457
                     /gene="miaB"
                     /locus_tag="VM910_20510"
     CDS             3102..>4457
                     /gene="miaB"
                     /locus_tag="VM910_20510"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HVI64942.1"
                     /translation="MKTPRKLHIKSYGCQMNVYDAQRMVDTLQAEGFVETDSAEDADL
                     VILNTCHIREKASEKVYSELGRVRLAKEQAARSGREMRIAVAGCVAQAEGEEITRRAP
                     VVDVVVGPQSYHHLPQLLARAESGGKAIETDFPAEDKFNFLAQPKPDAIRARGISAFV
                     TVQEGCDKFCTFCVVPYTRGSEVSRPVAKIVDDVKRLADNGVREVTLIGQNVNAYHGE
                     GPDGRSWPLGKLLHRLAEISGIARLRYSTSHPRDVEESLIEAHRDLKALMPFVHLPVQ
                     SGSDRILAAMNRRHTVRDYLDVIDRFRRARQDIAFSSDFIVGFPSESEQDFSATLALV
                     AQIRYAAAYSFKYSPRPGTPAAEMRETVSTADMDHRLVRLQELIDSQQSAFNKAAIGT
                     TVDVLFERAGRNAGQIVGRTAYLQPAHVMASPDIIGEILPVRLESLERYSLLGELAAP
                     AP"
BASE COUNT          848 a         1419 c         1383 g          807 t
ORIGIN      
        1 cgaactcgcg gacgagcaag acgagttcta cagtgaagag gacgccgaca cggtcgcgca
       61 gatcaaggat ctgatcgaga cgcgggtccg gccggcggtc gccaatgatg gaggcgacat
      121 caccttccgc ggcttcaagg acggcgtcgt ttacctcaac atgaagggcg cctgctcggg
      181 ctgcccgtcg tcgaccgcga ccctgcagca cggtatccag aacctgctca ggcattttgt
      241 gcccgacgtg gtcgaagtcc ggccaatgtg acggccctcg cctcaagcga ggaacgtatg
      301 cgacgaagca gtcgcgagac cgtcatcccg gggcgatgcg cgagcatcgg accagggatc
      361 tcagggctcc ggctcgattg cgacggcacg ttccggaaag gggataacgg caggccttcg
      421 cctccgtggg catgaccctg caattgcggg tctgctatat aatcagccga tgctgatcct
      481 cgccatagat accgcgctcg acgcctgcgc ggctgccatt ctcgacaccg gcaccagcaa
      541 gctgatcgcg atggaatcgc tgtcgatgaa gcgcgggcat gccgaagcgc tgatgccgct
      601 gatcgcgcgc gtcatgaagg acgcggcgct gccctttgtc gcgctcgacc gcatcgcagt
      661 taccaccggt ccagggagct tcaccggcct gcgcgttggg ctttcggcgg cacgcggtat
      721 tgcgctcgcc gcgggcaagc cggcggttgg actcaccacg ctttcggcct atgcggcgcc
      781 gatcgtcagc gagggcggcg aggagcccgt gatctcggcg atcgatgcgc ggcatgatca
      841 ggtctacttc caggcggtca gcggcaacgg cagttcgctg gtcaggccgc gggtcggcaa
      901 gatcgaggag gcccttgcag cctcccgttt cggcgcgccg catctggtcg gcaacgccgc
      961 caaaattctg gccgaccgct ggcccaagga cgcgccacaa ccggtccaga tcgatgcaca
     1021 agcagcgccc gacatctcct gggtggcctg gctcggtgcc gcagtcagcc ccgacacagc
     1081 gcccgctcgg cccttctatc tgcgcgctcc cgatgccaag ccctcgcaag acgtcacgcc
     1141 tccgcaacct gcgacctcat catgagctgg ctctccgagc tgctcggcac cggcacgccg
     1201 gtgatcgaac cggcgacaat ccgcgacgcg ccaagactgg cccagcttca cggcacctcg
     1261 ttccatcgcg gctggggcga gagcgaattc gagcggatgc tcgccgagcg taacacgctg
     1321 gttcaacgcc tgcggcttcg acgaaagatc atcggcttcg ccgtgtcgcg gatgggcgct
     1381 gatgaggccg agatcctgtc gatcgccatc gatgccggcc atcgcggaaa gggtttttcc
     1441 cgcaacctgc tgctgaccca tctcggccat ctctcaggat acggcgtgcg cagcatattt
     1501 ctggaggtcg aagagaataa ccacccggcc cgtcggcttt accaatgggc cggatttgcc
     1561 gtggtcgggc gccgcgagcg ctattaccag caagacgggg aacaactgaa cgcgcttctg
     1621 atgcggcgcg acttgtcgta agatgtggtg gtgtcgccga tttgaagtcc ccaccgtgcg
     1681 agcggcgagt ttgatacagg gacttcaaaa cgaaagcggc actgggctaa tatgcctgct
     1741 ggtgtccatg actttcggaa gctcgcggag cgaaggtgcc gcaacggacc gcgaacttcg
     1801 gaaagtgggc acgagacggc aaaaggtctt tctctcaagg cgatcctgat gaccgtattg
     1861 aaatcaactt ccgcgccaaa gccctccggc atcgaggcgc gctgtgccgc aaccggtatg
     1921 cggatgaccg agcagcgccg ggtcattgcg cgggtgctgg cggacgccgt cgatcatccc
     1981 gatgtcgagg agctgtatcg tcgctgcgtt gccgtcgacg acaagatctc gatctcgacc
     2041 gtctatcgca ccgtgaaact gttcgaggat gccggcatca tcgagcggca cgattttcgc
     2101 gagggccgcg cccgctacga gcagatgcgc gacagccacc acgatcatct gatcaacctg
     2161 cgcgacggca aggtgatcga gttcacctcc gaagaaatcg agaagcttca ggccgaaatc
     2221 gcccgaaagc tcggctacaa gctggtggac caccggctgg agctttattg cgtcccgctc
     2281 gacgatggcg gcacttgaga gcgcgcgcga cccggtgaca atcgatctca tcatcttcga
     2341 ttgcgatggc gtgctcgtcg acagcgagat catttcctgc cgcgcgcatg ccgcgacgct
     2401 gacgcgctac ggctatccga ttacgcctga gcaggttttc cagcgtttcc tcggccgatc
     2461 cgtgaggcaa gccaatctcg aggtggaggc cgagctcggt tgtgccctgc ccgacgacta
     2521 ccacaccgaa ctgcaggacg aggtctaccg ctgcttcgag gccgagctcg cacccattcc
     2581 gcagattcat caaacgctcg accagatcga acttccggtc tgcgtggcgt caagcggctc
     2641 gcatcaacgc atgagaatca gcctaggccg gaccggcctc tatgaaagat tcgcaccgca
     2701 tattttctcc gcctccgaag ttgcgaacgg caagccggcc ccggatcttt ttctgcttgc
     2761 tgcaaaacgg atggctacac cgcccgcgcg ttgcctggtc atcgaggaca gcgtgcccgg
     2821 catcgccggc gcgcgctcgg ccgggatgaa cgtgctgggc ttccacggcg gcagccattg
     2881 cgggcacgat catgccgcca cgctgcgcgc tgcgggcgct cacggcacct tcggtgatat
     2941 gcggcaattg ccgggcctta tcgcgcagaa ggccgcgaat cccgaccggg gcgagggagc
     3001 gtgaacttcc gaaaatggtc atcagtcgct ggattttcaa agatttaatc tatatttgag
     3061 ccggacgccg acttcggcgc cgtttccact tttcaggttc catgaagacg ccgcgcaagc
     3121 tgcacatcaa gtcctatggc tgccagatga acgtctacga tgcccagcgc atggtggaca
     3181 cgctgcaggc cgagggcttc gtcgagactg acagcgcgga agatgcggat cttgtcatcc
     3241 tcaacacctg ccacatccgc gaaaaagcgt ctgagaaagt ctattcggaa ctcgggcgcg
     3301 tgcgccttgc gaaagagcag gctgcccgca gcggccgcga gatgcgcatt gcggttgcgg
     3361 gctgcgtggc gcaggccgag ggcgaagaga tcacccgccg cgcgccggtc gtcgacgtcg
     3421 tggtcgggcc gcaaagctac caccatctgc cgcaactcct ggcgcgcgcc gaaagcggcg
     3481 gcaaggcgat cgagaccgat ttccccgccg aggacaagtt caacttcctg gcccagccga
     3541 aacccgacgc gatacgcgct cgcggcattt cggctttcgt cacggtgcag gaaggttgcg
     3601 acaagttctg caccttctgc gtggtgccgt atacgcgcgg cagcgaggtg tcgcggccgg
     3661 ttgcaaaaat cgtcgatgat gtgaagcggc ttgccgacaa tggcgtgcgc gaagtcacgt
     3721 tgatcggaca gaacgtcaat gcctatcacg gcgaaggtcc ggatgggcgt agctggccgc
     3781 tcggcaaatt gctgcatcgc ctcgccgaaa tttccggcat cgcgcggctg cgctattcga
     3841 cgagccatcc acgcgacgtc gaggaatcgc tgatcgaggc gcatcgagac ctcaaggcgc
     3901 tgatgccttt cgtgcatctg ccggtgcagt cgggttccga ccggatcctt gcggcgatga
     3961 accgccggca taccgtccgc gattacctcg atgtcatcga ccgtttccgc agggctcgcc
     4021 aagacattgc attttcatcg gattttatcg ttggcttccc aagcgagagc gaacaagatt
     4081 tttccgccac actcgcgctg gtcgcgcaaa tcagatacgc tgccgcctat tcgttcaaat
     4141 attcgccacg gccaggaacg cccgcggcgg agatgcggga gacggtttca acggcagaca
     4201 tggaccatcg gttggtgcgg cttcaggaat tgatcgacag ccagcaatcg gccttcaaca
     4261 aggctgcgat tggtaccacg gtcgatgtgc tgttcgagcg cgccgggcgc aatgcaggcc
     4321 agatcgtggg acggaccgct tatctgcagc cggcgcatgt gatggcctct cccgacatca
     4381 tcggcgaaat cctgccggtc cgcctggaaa gcctcgaacg ctacagcctg ctcggcgaac
     4441 tcgccgcgcc cgcccca
//
LOCUS       DATLJT010000890         3425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_975844,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000890 DATLJT010000000
VERSION     DATLJT010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975844"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1355)
                     /locus_tag="VM910_20515"
     CDS             complement(267..1355)
                     /locus_tag="VM910_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI64943.1"
                     /translation="MKRREFLKAAGLGMGASAVAAPAIAQSLPQLRWRLTASWPKSLD
                     TLYGAAEHMAKRIAELSENKFQIQVFAAGEIVPGLQVLDAVQNGTVEMGHTGSYYYFG
                     KDPAFTFGTALPFGLNTRQQEAWQFQGGGMELLNDFFKTYNCWGMPTGNTTCQMGGWF
                     RREIKSLDDMRGLKFRVGGFAGRILAKLGVVPQQLAAGDIFPALEKGTIDAAEWVGPY
                     DDEKLGFVKIAKYYYYPGWWEGGAQPHLFVNLGKWNEMPRIYQSMIQACAREAGAWMT
                     TKYDVLNPQAVKRLVASGAELRPFPQPVMEACYNAANEIYAELAKNNPHFKKMLDSLT
                     AYRSDSYQWLQVAELSYDTFMMRMRTRA"
     gene            complement(1352..1546)
                     /locus_tag="VM910_20520"
     CDS             complement(1352..1546)
                     /locus_tag="VM910_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64944.1"
                     /translation="MTAKMKRRQFITLLGGAAAWPLAAQAQQPAVPVIGLVGDGGGDI
                     STRYITAFRRGPDETREDQP"
     gene            complement(1543..2520)
                     /locus_tag="VM910_20525"
     CDS             complement(1543..2520)
                     /locus_tag="VM910_20525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64945.1"
                     /translation="MNKREFITLLGGAAGWPLIADAQQWTQPVIGFMSSRSPEDSEGV
                     VAAFRKGLSEGGLVEGRNVLLEFRWARGEYNRLPSLAAELVNQSVTVLVAAGGEASAL
                     TAKAATSTIPIVFISSDPVKSGLVASLNRPGGNATGFHFITTDLESKRLGLLHELFPG
                     TAILGGLVNPKSPPASAQAVEINEAARKLGRTVILLNATTDSELNAVFGSLSQRHVTA
                     MLVAADPFFDTRRDQLILLAAKYSVPIVYPMREFALAGGLMSYGTSASEAYHQVGIYA
                     AKIVNGAKPAELPVMQSIKFEQVINLKTARMLGFEFPPTFSARADEVIE"
     gene            complement(2517..>3425)
                     /locus_tag="VM910_20530"
     CDS             complement(2517..>3425)
                     /locus_tag="VM910_20530"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64946.1"
                     /translation="AQQPEKMLRVGALSAQPRTAPIWLAFERRMAELGYKHGTNFALE
                     FVRVDSMGDYERGYRELVARKVDIMLANGPEIALKSARAATQTLPMVMLAIEYDPIAR
                     GYVTNLARPSGNITGIFLQQIELAEKRVQILKDAFPDLRAAIAFWDGTSADQWDAAQT
                     AATTLGFQLAGAEMHDPPYDYERALAQSAPDHRGSLIVMVSPFFFRDRARLAEFALRH
                     RIVSMFGFREWVEAGGLLSYGPNIVELHRRVADYVDRIAKGAKPADLPIEQPTKFEFV
                     INLKTARAIGVDMPTSLLLRADEVIE"
BASE COUNT          672 a         1045 c         1000 g          708 t
ORIGIN      
        1 ctggagagca tttgtccttg ctaaagagga ccggcgtggt gccaataaga atgagattac
       61 catttttgtc tttagcataa ccgcgaagga cttttctcat ggcgtgctcc tggatggaaa
      121 agggacgatc cgtcgctctc ggcgagcccc tgtaagaggc cgatccgacg catctgctcg
      181 gactgctggg cgcgcgcagc gagcggccac gccgctgctg tgccgacgct ccggggcctt
      241 ccggcgatcg acacccaatg cgagcgtcag gcacgcgtgc gcatgcgcat catgaaggta
      301 tcgtagctga gctcggccac ctgtagccat tgataggaat cgctgcgata ggcagtcagg
      361 ctatcgagca tcttcttgaa atgcggattg ttcttcgcca gctcggcgta gatctcgttg
      421 gccgcattgt agcaagcctc catgaccggt tgcgggaacg gacgcagctc ggcgcctgaa
      481 gcgaccagcc gcttcaccgc ctgcggattg agcacgtcgt atttcgtcgt catccaggcg
      541 ccggcctcgc gcgcacaggc ctgtatcatc gactgataga tccttggcat ctcgttccac
      601 ttgccaagat tgacgaaaag atgcggctga gcgccgccct cccaccatcc gggatagtag
      661 tagtatttcg cgatcttgac gaaaccgagc ttctcatcgt catagggacc gacccattcg
      721 gcggcatcga ttgtgccctt ctcgagcgcg ggaaagatgt cgccggcggc aagctgctgt
      781 ggcacgaccc cgagcttggc caggatgcgg ccggcaaagc cgccgacgcg gaacttcaac
      841 cccctcatgt cgtcgaggct cttgatctcc ctgcggaacc agccgcccat ctggcaagtc
      901 gtgttgccgg tcggcatgcc ccaacagtta taggtcttga agaagtcgtt gagcagctcc
      961 ataccgccgc cttggaactg ccaggcctct tgctggcgag tgttgagccc gaacggcaaa
     1021 gccgtgccga acgtgaacgc cgggtccttg ccaaaatagt aatagctgcc tgtatggccc
     1081 atctcgaccg tgccgttctg caccgcgtca agcacctgca ggccgggtac gatctcgcct
     1141 gcggcgaaca cttggatctg aaacttgttc tcgctgagtt cagcgatccg cttggccatg
     1201 tgttcggccg cgccatagag cgtgtcgaga gacttgggcc agctcgccgt cagccgccac
     1261 ctcaactgcg gcagggactg agcaatggcc ggagcggcga ccgcggatgc tcccatcccg
     1321 aggcccgccg ccttgagaaa ttcccgtcgt ttcatggttg atcctcccta gtctcgtcgg
     1381 ggcctctgcg aaaggcagtg atatagcgcg ttgagatatc gccaccaccg tcgccgacga
     1441 gtcctataac gggcaccgcc ggctgctgcg cttgcgccgc gagcggccac gccgccgcgc
     1501 ccccgagcag cgtgatgaac tggcgtcgct tcatcttcgc ggtcattcga tcacctcgtc
     1561 ggcgcgggcg ctaaacgtcg gtggaaattc gaagccaagc attctcgcgg tctttaagtt
     1621 tattacctgc tcgaacttga tggattgcat tactggcagc tctgcaggct tggcgccatt
     1681 aacgatctta gctgcataga tgccgacctg gtgatatgcc tcgctcgcgc tggtcccata
     1741 gctcattagg ccaccagcca acgcgaattc gcgcatagga tatactatgg gtaccgaata
     1801 ttttgcggcc aacaggatga gctggtcccg acgtgtgtcg aagaaagggt ctgccgcgac
     1861 cagcatcgcc gtgacatgac gctgcgatag agacccgaaa acggcgttaa gctcggagtc
     1921 ggtagtggcg tttagaagaa tgaccgtccg accgagcttg cgcgccgctt cgttgatctc
     1981 caccgcctgt gcggaggccg gtggtgattt cgggttgact aagccgccca atatggccgt
     2041 gccaggaaaa agttcatgca ggaggccgag ccgttttgac tccagatcag tggtgataaa
     2101 atggaagccg gtcgcgtttc cacctggccg attcaggctt gcgaccaagc cggatttcac
     2161 cggatcgctg cttatgaata cgattggtat ggttgaagtt gcggcctttg ctgttagggc
     2221 ggaagcttcg ccaccagcgg caacaagtac agttacgctc tgattgacaa gttctgcggc
     2281 gagactcgga agtcggttgt actcgccgcg cgcccaacgg aactcaagca gcacgtttcg
     2341 gccttcgacg agcccaccct cgctgaggcc tttgcggaac gcggcgacca cgccctctga
     2401 gtcctcaggc gaccggctgc tcatgaaccc gattaccggc tgcgtccact gctgcgcgtc
     2461 agcgattagc ggccacccag ccgcgccgcc gagcagcgtg atgaactcgc gcttgttcat
     2521 tcgatcacct cgtcggcgcg cagtagaaga gacgtcggca tatcgacgcc gattgctctg
     2581 gcggtcttga ggttgatcac aaattcgaac ttagtcggct gctcaatcgg caggtcggca
     2641 ggctttgctc ccttcgcgat ccgatccaca tagtcagcca cccgacggtg cagctcgacg
     2701 atgttaggtc cgtaggatag caagccaccc gcctcgaccc attcgcggaa gccaaacatg
     2761 gagacaatcc gatgtcgcag cgcaaattcg gcaagccgcg cgcggtctct aaaaaagaac
     2821 ggagacacca tcacaatgag gctgccgcga tggtcgggtg ccgactgagc aagcgcccgc
     2881 tcatagtcat agggagggtc gtgcatctcg gcccccgcaa gttggaaccc aagcgtagtc
     2941 gcagcggttt gcgccgcgtc ccattggtcg gctgatgtcc catcccagaa tgcgattgcg
     3001 gcccgtaagt ccgggaacgc atctttcagg atttgcacac gcttctccgc aagctcgatc
     3061 tgttggagaa aaatcccagt aatgttgccg gaaggtcgag cgaggtttgt cacatagccg
     3121 cgggcaatcg gatcgtattc aatcgcaagc ataaccattg gcagtgtttg cgtggctgcg
     3181 cgcgcagact tcagagcgat ctccgggccg ttggcgagca taatatctac tttgcgcgct
     3241 accagctccc ggtagccgcg ttcgtagtcc cccatgctat cgacccgcac gaattcgaga
     3301 gcgaaattcg tcccgtgctt atagcccagc tcggccatgc gccgctcaaa agctagccaa
     3361 atcggcgctg ttcttggctg ggcgcttagc gcaccgacac gcagcatctt ctccggctgc
     3421 tgcgc
//
LOCUS       DATLJT010000891        18163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2125751,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000891 DATLJT010000000
VERSION     DATLJT010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18163
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125751"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VM910_20535"
     CDS             <1..772
                     /locus_tag="VM910_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI64947.1"
                     /translation="SDELLAGGGTGGSKSIMHSGTAIVEASGKVIEQGKQIASHVLEA
                     SAADIDFAQGRFVIAGTDRAIGIMELAERLRAGLKLPEGVPSTLDVRHVSDGPGASTY
                     PNGCHVCEVEVDPDTGVIEVVKYVSVNDFGTVINPLIVEGQLHGGVIQGVGQALMEMT
                     VYDGDGQFLTGSFMDYALPRASDSPEVTVINHPVPAKTNVLGVKGCGEAGCAGSLTSV
                     MNAIAHALSEFGIRHMDMPATPYRVWRAIQDARAAHAA"
     gene            800..2026
                     /locus_tag="VM910_20540"
     CDS             800..2026
                     /locus_tag="VM910_20540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI64948.1"
                     /translation="MAQAAELLTPARASETKLVAGVCFAHLVSHYYILILAPLFAFIR
                     TDFGVTYTELSLALTTFNVVSAVLQTPVGFFIDRTGARINLIGGLLLGSAAIAIAGTV
                     HSFWVFIAMFAVLGLANTVYHPADYTLLSERVSPRRVTQVFSFHTCAGMIGSAIAPVT
                     LLFLQSAVGWRGAFLCSALLGVVAAVIVAVQGEAPVVHPAHPSKPRGEEATSAAADGW
                     QFLLSPPILLNLLFFVVLSMVGGGLNQYLVVGLGALYGTPPALANTALTGLLTMSAIG
                     VLLGGALAGRTVRHNLVACAGLVVTGTASTLIAFVDPGALVLVLIASLSGFASGMTMP
                     SRDMIVRSVTPPGSFGKVFGFVTTGFHIGGMVAPLIFAQFLDHGYPRGVFLYVAACAL
                     VAIATVAFGMSGRRTA"
     gene            2250..2323
                     /locus_tag="VM910_20545"
     tRNA            2250..2323
                     /locus_tag="VM910_20545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2282..2284,aa:Gln,seq:ctg)
     gene            2366..2809
                     /locus_tag="VM910_20550"
     CDS             2366..2809
                     /locus_tag="VM910_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64949.1"
                     /translation="MRYLVFALVLLGAAATSARAQDAIPDLKGTWSGKGKSIVFGTNP
                     HHPGSQAATDPPRVRDIEATYIVDGQEGRLAWGRSSSTAANTQEPFAWAIASDNKTIV
                     GADMDGYFRITLISPDSIEKCYTHNGTSPTHAIVATCHVMNRVRR"
     gene            3209..4306
                     /locus_tag="VM910_20555"
     CDS             3209..4306
                     /locus_tag="VM910_20555"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HVI64950.1"
                     /translation="MTRATELELLDSKKKMISGTYLRNAWYVAAWSDDLADGQLLNRT
                     ILKEPVVLFRKSDGNVAALQDRCPHRFAPLHMGKIVDGDRVQCPYHGLEFDSFGACVL
                     NPHGTKNIPPRARVRSYPVTEKHKAIWIWMGEQSPDHAKVPDFSVLDNVPEMHATKRD
                     RITIRANYELIIDNLLDLSHTSYLHDGILGNADTVESEITVEQDGDDVVVGRYARNAA
                     APGLFAILMPSTPERVDKFTRMRWMAPSNLRLLTGICAPGTVPESGTGYHAIHMLTPE
                     SDKTTHYFFTAVRFNVLTADDRLNAQIKDKISTTRRFAFEEQDAPVIEAQQEMIDAAA
                     TAVDPIMLAIDVGPVRYKQILQKLLRAEQRQ"
     gene            complement(4441..5154)
                     /locus_tag="VM910_20560"
     CDS             complement(4441..5154)
                     /locus_tag="VM910_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HVI64951.1"
                     /translation="MSTRGHVVAVTCLALEARIALGPGVAVICNHASQLVASLEAAAK
                     RGAAGIISFGIAGGLAPDLDAGDWVVGSGVRTEHGNFPTDRGWARALLEALPSAVHAE
                     IVGTDTPIAEPEEKRRLHLRTGAVAVDTESHVAARIAAAHRIPFAACRAVIDPAHSVL
                     PPAAMCGLRHDGTADVFAVLRSVVRQPSQLPALTRTALDARVAAAALRRGRRLLGAGL
                     GFPYFSEPALDGRMAVEMG"
     gene            5701..7095
                     /locus_tag="VM910_20565"
     CDS             5701..7095
                     /locus_tag="VM910_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HVI64952.1"
                     /translation="MPTWTPDPSFYPSPRQAAKAPPESLAYVAAFDPDRKSPDRIAVV
                     DVDPKSSSYSKIIGNVAATEVGDEFHHFGWNACSSCLCPNAPHPHVERRYLVVPGLRS
                     SRIYILDTKPDPRAPKIVKVIEPAELADKTGYTRPHTVHCGPGGIYVAALGNKDGKAP
                     GGVFVMDHESFEPLGRWEVNRGPQQLGYDAWWHLGYDTMVTSEWGTPDTFENGLVPEI
                     LLGSKYGRRLHFWDFTRRTHLQEIDFGEEHQLVFELRPAHDPTKAHGFVNCVVSLKDL
                     SSSIWTWFREGNKWAAKKIISIPAEPANPDDLPPVLKGFKAVPPLVTDIDLSMDDRFL
                     YVACWGTGDLQQYDVSDPFNPKLTGKVRIGGIVSRATHPGAKNGKLNGGPQMVEVSRD
                     GRRVYFTNSLYGAIDPQFYPEGIDGWMVKLDAGPAGGIAFDPKFFIDWPKGHRPHQVR
                     LQGGDCSSDSYCYP"
     gene            7142..7777
                     /locus_tag="VM910_20570"
     CDS             7142..7777
                     /locus_tag="VM910_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64953.1"
                     /translation="MSAVPTEVWPWLALAGLGLFHGLNPAMGWLFAVALGLHRGSQRI
                     VLLSLVPIAVGHAAAVAAVLLAVLALGLVIDHTMLIRLAAIVLIAWAAWHALYGHQRR
                     VRVGMQTGLLGLVLWSFLMASAHGAGLMLIPAMEPICASAASAPDASSSTHLALAALG
                     VHSAAMLATIAAVSLVVYHWTGVDFLRRGWVNLDLIWVAVLALCGAILLFA"
     gene            complement(7902..10979)
                     /locus_tag="VM910_20575"
     CDS             complement(7902..10979)
                     /locus_tag="VM910_20575"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HVI64954.1"
                     /translation="MTRFSGSKFAAALQPRGVAKYAFELAIIGASYFVLAKVGLTLAS
                     IYSSAVPIWPPAGLALAAVLLRGLRIWPAIFAAAFAVGVPTDIADARVADSILLSLGV
                     AAGNTLEAIVGGCLVNAWSQGRKTFDTAAGVAKFAVVGLGPSAMIGAIVGAGSLYLAG
                     DVDWGNFIAIGATWWLRDAAGALVITPVVVLWAIEDFRGFNLDKVLACGATIVAATAV
                     GLIAFSPLIEQSVNRSALGFLAAVPLLWAALRCGGRDTATAVLILSCFAAWGTLAGGG
                     PFAGATLDDAFLPLIAFMLSISVVSLALSAHVAVRKRIETKLRQQEHILRAMFSQSVA
                     GIAQIDTAGRFTLVNNGYCGLVRRPAPELLQMGIQDLTAPDDLPYIRNLMGQAIRSGE
                     GFAIETRNVLPDGSRLWVRNNVSAITDRSGAVRHLMVVAEDVTDRRRAEEDLQRAHDD
                     LRKMVAERTVTLKQTTEVLHTEIEQRKRVEAALKHDIAERRKAQEALMESEWRFRTVI
                     QGITDYAIFMLDRGGYITNWNVGAQRIHQYTAAEIVGQHFSRFYSEEEQYRGEPARAL
                     QVAAYEGKCAVEGWRVRRDKSLFWASAVIEAVRDEAGTLAGFVNITRDITERREAQAS
                     LERAQEQLAQSQKMEALGQLTGSIAHDFNNLLMIVSGHAQLLRRRLSDPKHLQAIDAV
                     HSAANRGESLTRQLLAFSRRQPLNPVVTDLKERIEAVHEMLVGSLRGNVQLKCDIPAD
                     VWPVEVDIAELELALVNIAVNARDAMPGGGLIKLSAQNVSLKKSDGVGQLEGDFVALA
                     MTDTGVGIAPDVLPRIFEPFFTTKALGKGTGLGLAQVYGFSHQSGGTVVATSAVGSGT
                     TITIYLPRRQAAPVEAAVQAPPTQPMVPGQGTILVVEDNAEVADITASLVEQLGYQTA
                     RAGNAAEALNRLRRGDKVDLVLSDVVMPGGMNGIALAQEIGNHYPHIPVVLTSGYNDV
                     VQTAQNRFAILRKPFQLSALEKSIREALERGATRDDGERVLQLSRWRGTTGRE"
     gene            complement(11362..11964)
                     /locus_tag="VM910_20580"
     CDS             complement(11362..11964)
                     /locus_tag="VM910_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64955.1"
                     /translation="MRTKSFLSRFAVKFVEVAGAGIASAACAYIFGQIERPQPPSAPV
                     VQIAPASVERARMTQNDRPLVGETARTETENKQENVAATPTAVWAPKLAQPAQAALPR
                     RNQKSEQGPLAEAKARSGEPLAISPSRVASNSAPKAAGQSVQAPVAYGGAASNSGEEE
                     RPLLARLRQIPSWFLPENDRIFGELPRPPMPVGEFLQSSM"
     gene            complement(12159..12806)
                     /locus_tag="VM910_20585"
     CDS             complement(12159..12806)
                     /locus_tag="VM910_20585"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HVI64956.1"
                     /translation="MPRTAKVTRVSKATRDKIIKAASRAFAVNGYEGASIRAIVAEAD
                     VNQAAINYHFGSKEGLYRAVLQTALRALMKDDDAPQNPDILPRETALRRFVRRQLRPM
                     TARDELSEYVRIFSWETLKPSPVFRKFMARETAPFFAAATELVRRFLPNNATDQQAVV
                     GALWLFGQCSVFVRNAEQLANPPLRLKVDEGFVDRLADTIATWAADGLGRLPTRS"
     gene            complement(12950..13279)
                     /locus_tag="VM910_20590"
     CDS             complement(12950..13279)
                     /locus_tag="VM910_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64957.1"
                     /translation="MVVAERRLIVTGPSGDTEVSVRLFQPENEEGMWICRYEIDWPNE
                     RRSYFAGGVDSFQALILALQMIGAEIDASAYHKAGSLKWFERYRGYGFPVGCNLRDLL
                     IGDDTNL"
     gene            complement(13502..13627)
                     /locus_tag="VM910_20595"
     CDS             complement(13502..13627)
                     /locus_tag="VM910_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64958.1"
                     /translation="MSREAAKKIVDLMIKHGAEQNAVLAENNPFARMMNFKDINE"
     gene            complement(14496..15506)
                     /locus_tag="VM910_20600"
     CDS             complement(14496..15506)
                     /locus_tag="VM910_20600"
                     /inference="COORDINATES: protein motif:HMM:NF037424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD23 domain-containing protein"
                     /protein_id="HVI64959.1"
                     /translation="MTTPTVPQGSSTKPSPPFYGPGDVLLPPPEPAPPPAPKDFRTWL
                     RDALSDENVRYYAGPHLYDALLKLHALTQLLPGSGTVQSTQDASLAGEEAKAGNYGKA
                     AAHLGLGTVNAALDWLPPTKLAIIGGTMARTFPWDKLPTAMKMEAAGKSADEIWRMTG
                     LERAADERWTFEIPDKGYQVNPNAGERIGTQSLTVAPLYEHHAHPGMQEAYPGLANWQ
                     SRLTIDPLENPRGATDVNRNWVAVHAPSLEWARKVGIHELKHVIDKIEKHPPGGSPDQ
                     FMKLRIPEQEAYDLYNRLVGEVAARNAQKRLDWGDIIRRYRRPGATEDVPRHRQINLY
                     DE"
     gene            complement(16400..17860)
                     /locus_tag="VM910_20605"
     CDS             complement(16400..17860)
                     /locus_tag="VM910_20605"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVI64960.1"
                     /translation="MSSSQERTARSFVERHGLWSDEQFEAAARADKWIEEQGLEVVRL
                     SFPDQHGILRGKTMVANEAAAAMRNGCSITTTLLAKDTAHKTAFPVFTPGGGFGMTEM
                     EGGGDFLMIADPLTFRLLPWAPRTGWVLCDIYFATGQPVPFSTRHLYRRVLQRLAEAG
                     FDYVAGLEVEFHVFKLDDPRLMPADATWPGEAPRVSLLSQGYQYLTETRFDQFEPTLE
                     TLRREVVALGLPLRSVEVELGPSQCEFTFQPQVGLSPADTMMLFRSAAKQICRRQGCH
                     ATFMTRPKVENVMSSGWHLHQSLRDRRSSANVFMSNDDDGLSRAGRSFLAGLLAHAHA
                     AAAFTTPTINGYKRYRGYSLAPDRVIWGRDNRGAMIRVLGGPGDAATRLENRVGEPAA
                     NPYLYMASQVVTGLDGMERMLDPGPSADAPYEVEAPHLPKSLEQALAALREDACLAAG
                     FGKNFIDYYLRIKEAEIARYHSEVTEWEQREYFELF"
     gene            complement(17935..>18163)
                     /locus_tag="VM910_20610"
     CDS             complement(17935..>18163)
                     /locus_tag="VM910_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HVI64961.1"
                     /translation="APSHGRILAVDPAIATAHTGHGIVMPQCNHGGPGRAGAGEELGG
                     LYGLRFYHQRLAVQGSSDLIASLQAKAASVH"
BASE COUNT         3326 a         5772 c         5658 g         3407 t
ORIGIN      
        1 cagcgacgag ctgctcgccg gcggcggtac cggcggctcg aagtcgatca tgcacagcgg
       61 cacggcgatc gtggaagcat ccggcaaggt gatcgagcag ggcaagcaga tcgcctctca
      121 cgtgctggaa gcatcggccg ctgacatcga cttcgcgcag gggcgcttcg tcatcgccgg
      181 caccgatcgc gcgatcggga tcatggagct cgccgagcgg ctgcgcgcgg gactcaagct
      241 gccggaaggc gtgccgtcga cgctcgatgt ccgccacgtg agcgacggcc ccggcgcctc
      301 cacctacccc aacggatgcc acgtctgtga ggtcgaagtc gatcccgaca ccggcgtgat
      361 cgaggtggtg aaatacgtct cggtcaacga tttcggcacc gtcatcaacc cgctgatcgt
      421 cgagggtcag ctgcatggcg gcgtgatcca gggcgtcggc caggcgctca tggagatgac
      481 cgtctacgat ggcgacgggc agttcctgac cggttcgttc atggactacg cgcttccgcg
      541 cgcgagcgat tcgcccgaag tcaccgtcat caatcatccg gtaccggcga aaaccaacgt
      601 gctcggcgtc aagggctgcg gcgaagcggg ctgtgccggc tcgctcacct cggtcatgaa
      661 tgcgatcgcc catgcgctgt ccgaattcgg catccgccac atggacatgc cggcgacgcc
      721 ataccgcgtc tggcgcgcca ttcaggatgc ccgcgccgcg cacgcggcct gatcggcccc
      781 gccgcgacta aaacaacaaa tggcccaagc tgccgaactt ctcacccccg cccgcgcgag
      841 cgagaccaag ctcgtcgccg gcgtgtgctt cgcccatctg gtgagccact attacatcct
      901 gatactcgcg ccgctgtttg cgttcatacg cacggacttc ggcgtgacct ataccgagct
      961 cagcctcgcg ctcaccacat tcaatgtggt ctcggcggtg ttgcagacgc cggtgggctt
     1021 cttcatcgac cgcaccggcg cacgcatcaa cctgatcggc ggcctgctgt tgggctcggc
     1081 cgccatcgcg atcgcgggaa cggtgcattc gttctgggtg ttcatcgcga tgttcgccgt
     1141 cttgggcctc gccaacacgg tctaccatcc cgccgactac accctgcttt ccgagcgcgt
     1201 ctcgcctcga cgcgtgaccc aggtcttctc gttccacacc tgcgccggca tgatcggttc
     1261 ggccatcgcg ccggtgacgc tgctgttcct gcagagcgcg gtgggatggc gcggcgcctt
     1321 tctctgctcg gcgctgctcg gcgtcgtcgc ggcagtgatc gtggcggtgc aaggagaagc
     1381 gccggtcgtg catccggcac atccgtcgaa gccacgcggc gaagaggcga cgagtgcagc
     1441 cgccgacggc tggcagtttc tgctgtcgcc tccgatcctg ctcaacctgc tcttcttcgt
     1501 cgtactgtcg atggtcggcg gcggcctcaa ccaatatctg gtggtggggc tcggcgcgct
     1561 ctacggaacg ccgcccgcgc tggccaatac cgcgctcacc gggcttctga ccatgagcgc
     1621 catcggggtc ttgctcggcg gagcgctcgc ggggcgcacc gtacggcaca acctcgtggc
     1681 gtgcgcggga ttagtggtga cgggcaccgc cagcacgctc atcgcgttcg tcgatccagg
     1741 cgcgctggtc ttggtcctga tcgcgtcgct aagcggtttc gccagcggca tgaccatgcc
     1801 ctcgcgcgat atgatcgtgc gctcggtcac gccgccgggc tcgttcggca aggtgttcgg
     1861 cttcgtgacc accggctttc atatcggcgg catggtggcg cccttgatct tcgcgcagtt
     1921 cctcgaccat ggctatccgc gcggggtgtt cctctacgtc gccgcctgcg cgctggtggc
     1981 gattgccacc gtggctttcg gcatgtcggg gcggcgcacg gcgtgagcgg cgtctgccta
     2041 acgcggtgag acagtgcgtc gatcgcaagg caccgtgtgg cgctcgtttc cacgcaaagg
     2101 ccggaccttt cgcgcttgga aagccgaccg agatgtccta gatgtgctgg cgtcgcgtcg
     2161 acgttcgaac accgcgccga ccaagagaca aggaacgagg cgacaggacg cgccccggaa
     2221 gctacactca aagttagcgt tttcccaatt gggggatagt tcaatggtag aacagcggac
     2281 tctgactccg ttaatctagg ttcgaatcct agtcccccag ccaatccaac tcggcgagcg
     2341 atccgcatca gcacgggagg aagcgatgag atatcttgtc tttgcgttgg tgcttctcgg
     2401 cgcggcagca acgtccgcgc gcgcacagga cgcaattcct gatctcaagg gcacctggtc
     2461 gggaaagggt aagtcgatcg tgttcggcac aaatccgcat catcccggtt cgcaagccgc
     2521 taccgatccg ccacgcgtgc gcgacatcga ggcgacctac atcgtggatg gtcaagaggg
     2581 ccggcttgcg tggggtcgct cgtcgtcgac cgccgccaac acccaagagc cgttcgcgtg
     2641 ggcgattgcg agcgacaaca agacgatcgt cggcgccgat atggacggct attttcgcat
     2701 caccctcatc tcgccggata gcatagaaaa gtgctacacc cacaacggca ccagcccgac
     2761 ccatgcgatc gtcgctacct gccacgtgat gaatcgcgtg aggcggtagg atttgtcgcg
     2821 catgccgaga gcgtagcgct gcgcccaggc tgcgacggcc agcgccacag cgttggggat
     2881 cgcgcataag gaaggccccg gtgaggcttt caccggggcc ttagcgctcc cgtgggctct
     2941 ttggggggca gtggggcctt tcgggaacgc cacaagatta tcgttcggca agcgatttgg
     3001 tccccgccga cgcgaatgga tcgttcgcga ccgcctccgt cagcgcaaag cgcatcgcgg
     3061 ctttgcgccg ttcgccacgc ccgagagggc ggcttgaccc gcaacgacgg cccgaaagat
     3121 aatccgctgc attcgcgcga ggcacgctgc ttcgccattg cgaagatcga gcttctgctt
     3181 aatcggcctc agacacggag gagccgcgat gacgcgcgcc acggagctcg agcttctcga
     3241 cagcaaaaag aaaatgatca gcggcaccta tctccgcaac gcgtggtatg tcgccgcttg
     3301 gtccgatgac ctcgccgacg gtcaattgct caaccgcacg attttgaaag agccggttgt
     3361 tcttttccga aaatcggacg gaaatgttgc tgcactgcag gatcgctgtc cgcaccgatt
     3421 cgcgcccctg catatgggca agatcgtcga cggcgatcgc gttcagtgtc cctatcatgg
     3481 gctggaattc gactcgttcg gggcttgcgt cttgaacccg catggaacga aaaacattcc
     3541 tccccgcgcc cgcgtccgca gctatccggt gacggagaag cacaaggcga tctggatttg
     3601 gatgggcgag cagtcgcccg accatgcaaa ggtgccggac ttcagcgtgc tcgataatgt
     3661 cccggagatg cacgcgacga aacgggaccg aatcaccatt cgcgccaact acgagcttat
     3721 catcgataat ctgctcgact tgagccatac ttcctatctg cacgacggaa ttctcggcaa
     3781 cgcggatacg gtcgaatccg aaatcaccgt cgagcaggac ggcgatgacg tcgtcgtcgg
     3841 tcgttacgcc cgcaatgcag ccgcgccggg actattcgcc attctgatgc cgtcgacgcc
     3901 ggagcgggtc gacaagttca cccgcatgcg gtggatggca cccagcaatt tgcgcctgct
     3961 gacgggaatt tgcgcgcccg ggaccgtgcc ggagagcgga accggttatc atgccatcca
     4021 tatgcttacc ccggaaagcg acaagaccac gcactatttt ttcaccgcgg tgcggttcaa
     4081 cgttttaacc gcggatgatc ggttgaacgc gcagattaag gacaaaatat cgacaacccg
     4141 ccggttcgcg ttcgaggagc aggacgcacc ggtgatcgag gcgcagcaag agatgatcga
     4201 tgccgcggcg accgcggttg atccgatcat gctcgcgatc gacgtggggc cggtgcgcta
     4261 caagcaaatc ctgcaaaaat tgcttcgcgc cgagcagagg caataggcga tgcgcgcgcg
     4321 ggaggaattc gcgcggcggg tcagcgtcga aatcgagatc cgggcaagat gatccgcgcc
     4381 gacatcacgg cgcagcaacg tcaaagctct cccgcaaaga ggtgaaggcc gggccggcac
     4441 tcagcccatc tcgacggcca tgcggccgtc cagcgcaggc tcgctgaaat acgggaagcc
     4501 aagtcccgcg cccaacagcc gtcggccgcg gcgcagcgcc gccgcagcga cgcgggcatc
     4561 gagcgcggtg cgcgtgagcg caggaagctg gctgggctgc cgcacaaccg agcgcaatac
     4621 cgcgaataca tccgctgtgc cgtcgtggcg cagtccgcac atcgccgccg gcggaagcac
     4681 gctgtgggcc ggatcgatga ccgcgcggca ggccgcaaat ggaatccggt gcgcggcggc
     4741 gatcctggcc gcaacgtgtg actcggtatc gacggcgacg gcgccggtcc gcaggtgaag
     4801 ccgacgcttt tcctcgggct ccgcgatcgg ggtatctgtc ccgacgatct cggcgtggac
     4861 ggcgctcggg agcgcctcga gaagcgcgcg tgcccatccg cgatcggttg gaaagtttcc
     4921 gtgctcggtc cgcactccgg atccgacgac ccagtcgcca gcatcgagat cgggcgccag
     4981 tccgccggca attccaaagc tgatgatgcc cgcagcgcca cgcttggccg ccgcctcgag
     5041 ggaggcgacg agctgggagg catgattgca gatcaccgcc acgccgggac caagggcgat
     5101 gcgtgcctcc aaagcaagac aggtgactgc gactacgtgc cccctagtcg acattcaacg
     5161 ctcgttctgt tcgcgtagcg cgtccgggta tttcgtcggt aatcttcccc agaaagtgaa
     5221 attggtcaac ggccttaccc tcgatctgcc catccgcgtg ggccgcgttg cttcggaaat
     5281 atcccgggtc gggcgcggat cccggcacgc atttgctact tttttcctcg ctcgatcggc
     5341 cacctttcgg cagtctcggc tgcggtatcg ggcgatttca cttcttggac atttctgacg
     5401 tccgcgcgcc catgccgttc aggtgacctg caatcctgga gcatactaga acggaccggg
     5461 tacaagcatc gatcctggct cgatatgccg cgtcgagact gcgggttggc gagctacaat
     5521 gcccaaaacg cacgggacgc cgccgcgcgc gcacacccga gggcgttacc gagtcgaaga
     5581 aaacgggggc cgcacgtaac cgattcggtc ggacttgggt tggtcaaccg cgacccgctt
     5641 gcgcaacgga acccaagcca ggacatgaga tgcaagccag tctcgagagg agagaatcac
     5701 atgcccacct ggacgcctga tccgagtttt tatccctctc cacgccaagc ggcgaaggca
     5761 ccgcccgaat cgctcgccta tgtcgccgcc ttcgatcccg accgaaaatc ccctgaccgc
     5821 attgcggtcg tcgacgtcga tccgaaatcg tcatcgtatt ccaagattat cggcaacgtg
     5881 gcggcgaccg aggtcggcga cgagttccac cattttggct ggaatgcttg ctcgtcatgt
     5941 ttgtgcccga acgcgccgca cccccatgtg gagcgacgct acctcgtggt cccgggcctg
     6001 cgttcctcgc gcatctacat tctcgacacc aagcccgatc cgcgggcgcc gaaaatcgtc
     6061 aaggtcatcg aaccggcgga gctcgcggac aagaccggct atacccgccc gcataccgtc
     6121 cactgcggac cggggggcat ctacgtcgca gctctcggaa acaaggacgg caaggcgccc
     6181 ggcggcgtct tcgtcatgga tcacgagagt ttcgaacccc ttggccgctg ggaggtcaac
     6241 cgcggaccgc agcagctcgg ttatgacgcg tggtggcatc tcggctacga caccatggtt
     6301 accagcgaat ggggcacacc cgacaccttc gagaacggcc tcgttccgga aattctgctc
     6361 gggagcaaat acggcaggcg cctgcatttc tgggatttca ccaggcgcac gcacctgcaa
     6421 gagatcgatt tcggtgaaga acaccagctc gtgttcgaac tccggcccgc gcacgacccg
     6481 accaaagccc acggcttcgt caattgcgtc gtcagcctca aggatctgtc ctcctcgatc
     6541 tggacctggt tccgcgaagg caacaagtgg gcggcaaaga aaatcatctc gatcccggcc
     6601 gagccggcaa atcccgacga tcttccgcct gtgctcaagg gcttcaaggc ggttccgccg
     6661 ctcgtaaccg acattgatct atcgatggac gaccgcttcc tctacgtggc gtgctggggc
     6721 accggcgacc tgcagcaata cgacgtgtcg gatccgttca acccgaagct caccggaaag
     6781 gtgcgcattg gggggatcgt gtcgcgggcg acgcacccgg gcgcgaagaa cggcaagctc
     6841 aatggcggcc cgcagatggt ggaggtcagc cgcgatggcc ggcgtgttta tttcaccaac
     6901 tcgttatacg gcgcgatcga tccgcagttc tatccggagg gaatcgacgg ctggatggtc
     6961 aagctcgacg ccggccccgc cggcggtatc gcgttcgatc cgaagttctt catcgattgg
     7021 ccgaaagggc accgaccgca ccaagtgcgc ctgcaaggcg gtgactgctc gtcggattct
     7081 tattgctatc cgtgatgtga ccaaatcggt gcaggtagcc gcacccaccg cgaggatcag
     7141 catgagcgca gtccctaccg aggtctggcc gtggttggcg ctcgcgggcc ttgggctttt
     7201 tcacggactc aacccggcaa tgggctggct gttcgcggtc gcactcggcc tgcaccgcgg
     7261 aagccagcgg atcgtgctgc tgtcgctcgt tccgatcgcc gtcgggcacg cggccgcggt
     7321 tgccgcggtg ctgcttgccg tgcttgcgct cgggctcgtc atcgatcaca cgatgctgat
     7381 tcggctggcg gcgatcgtgc ttatcgcctg ggccgcttgg catgcgctct atggccatca
     7441 acggcgggtg cgggtcggga tgcagaccgg cctgctgggt ctcgtcctat ggtccttcct
     7501 gatggcgagt gcgcatggcg ccgggttgat gctgatcccg gcgatggagc cgatttgcgc
     7561 ctccgcggca tcggcgcccg acgcgagctc cagcactcac ctcgcgctcg ccgcgctcgg
     7621 cgttcattcc gctgcaatgc tcgcaaccat cgccgcggtt tcactcgtcg tctaccactg
     7681 gacaggggtc gattttctgc gacggggatg ggtcaatctc gacttgattt gggtcgccgt
     7741 gctcgcccta tgcggggcca ttttactgtt cgcgtagcac gacaacagaa ccgtcggttc
     7801 tcaagcgcta gcgccgcaat tcgagaattg cgggctccca agcttcgatc acttgcgtga
     7861 ttgtggttga ccggccccgg cgggagcgcg cgtcgcccga ttcattcccg cccggtcgtg
     7921 ccgcgccagc gcgagagttg cagcacgcgc tcgccgtcgt cgcgcgttgc gccgcgttca
     7981 agcgcttcac gaatggactt ttccagcgcc gatagctgga acggcttgcg caaaatcgcg
     8041 aaccggtttt gcgccgtttg caccacgtcg ttgtagccgc tggtgagaac gacggggata
     8101 tgcggatagt ggttgccgat ttcctgcgcc agcgcgatgc cgttcatacc gccgggcatg
     8161 acgacgtcgc tgagaacgag atcgaccttg tcgccgcgtc gcagccggtt cagcgcttcg
     8221 gcagcgtttc ccgcgcgcgc ggtctgataa ccgagctgct cgacgagcga agcggtgatg
     8281 tcggcgacct ccgcgttgtc ctcgaccacg agaatggtgc cttgcccggg caccataggt
     8341 tgggtcggag gcgcctgcac ggcggcctcg accggcgccg cctgcctgcg cggcaggtag
     8401 atggtgatgg tggtgccgct gccgaccgcg ctggtcgcaa cgacggtgcc ccccgattga
     8461 tgggaaaagc catagacctg cgccaatccg agaccggtac ctttgccgag tgctttcgtc
     8521 gtgaagaacg gctcgaaaat ccgcggcagg acgtccggcg cgatgccgac accggtatcc
     8581 gtcatcgcaa gggcgacgaa gtctccctca agctgaccca ccccgtcgct cttcttcagg
     8641 ctgacgtttt gcgccgacag cttgatcaag cctccgccgg gcatggcgtc gcgcgcattg
     8701 accgcgatgt tgacgagcgc cagttcgagc tccgcgatgt cgacctcgac cggccaaaca
     8761 tcggccggta tgtcgcactt gagctggacg tttccgcgta gcgagccaac cagcatttca
     8821 tgcacggcct cgatgcgttc cttgaggtcg gtgacgacgg ggttgagcgg ctggcgtcgc
     8881 gagaacgcca ggagctgacg cgtcaagctc tcgccgcgat tggcggccga atgcaccgcg
     8941 tcgatcgcct gcagatgctt gggatcggag aggcggcggc gcagaagctg cgcgtggccg
     9001 ctgacgatca tcaacagatt gttgaagtcg tgggcgatgc tgcccgtcag ttggccgagc
     9061 gcttccatct tctgagactg cgccagctgc tcctgcgccc gctcgagcga tgcttgggcc
     9121 tcccgtctct cggtgatgtc gcgcgtgatg ttcacgaagc cggcaagcgt gcccgcctcg
     9181 tcgcggaccg cctcgatcac ggcgctcgcc cagaataggc tcttgtcgcg ccgcacgcgc
     9241 cagccctcga cggcacattt gccctcgtag gcggcgacct gaagcgcgcg cgcgggctcg
     9301 ccgcggtatt gctcctcctc ggaatagaac cggctgaagt gctgcccgac gatttccgcc
     9361 gcggtgtatt ggtgaatgcg ctgcgccccc acgttccagt tggtgatgta accgccgcgg
     9421 tcgagcatga agatcgcgta gtcggtaatg ccttggatga ccgtccgaaa acgccactcg
     9481 ctctccatca gcgcctcttg cgccttgcgg cgctcggcga tgtcgtgctt gagcgcggct
     9541 tcgacgcgct tgcgttgctc gatctcggta tgaagaacct cggtggtctg cttgagggtc
     9601 acggtgcgtt ccgcgaccat cttccgcagg tcgtcatgcg cgcgctgcaa atcctcctcg
     9661 gcgcggcggc ggtcggtgac gtcctccgcg accaccatga ggtgacgcac ggcgccgctg
     9721 cgatcggtga tggccgacac gttgttgcgg acccacaagc gcgagccatc gggcaacacg
     9781 tttcgcgtct cgatggcgaa tccctctccg ctgcggatgg cttgccccat gagattgcgg
     9841 atgtagggca ggtcgtcggg cgcggtcagg tcttggatgc ccatctgcag cagttccggc
     9901 gccggacgcc ggacgaggcc gcaatatcca ttgttcacca gggtgaagcg ccccgccgtg
     9961 tcgatctggg cgatgcccgc gacggactgg ctgaacatgg cgcggagaat gtgctcctgc
    10021 tggcgcaact tggtttcgat gcgttttcgc accgcgacat gcgcgctcag ggcgaggctc
    10081 acaaccgaaa tgctgagcat gaacgcgatc agcggcagga acgcgtcgtc aagggtcgcg
    10141 cccgcgaacg gcccgccgcc cgccaacgtg ccccaggcag caaagcacga gagaatgagc
    10201 acggcggtcg ccgtgtcccg ccctccgcac cgcagcgcag cccacaacag cggcaccgct
    10261 gccaggaacc ccaacgcgct cctgttcacg ctctgctcga tgagcggact gaaagcgata
    10321 aggcccaccg cagtcgccgc gacaatggtg gcgccgcagg ccaagacctt gtccaaattg
    10381 aagcctcgga aatcttcgat cgcccacagc acgacgaccg gcgtgatcac gagcgcgccc
    10441 gccgcatcgc gaagccacca tgtggcgcct atggcgatga agttccccca gtcgacgtca
    10501 ccggcgaggt agaggctgcc ggcgccaacg atcgcgccga tcatcgcgct gggtccgagg
    10561 ccgaccacgg caaatttcgc gactccggcc gccgtatcga aggttttgcg gccctgggac
    10621 caggcgttga ccagacagcc gccgacgatc gcttcaagcg tgttgcccgc ggcaacgccg
    10681 agcgacaaca agatcgagtc ggcaacgcgc gcatcggcga tatcggtcgg tacgccgacg
    10741 gcaaatgccg cggcaaaaat cgccggccaa atgcgcaggc cgcgcaagag gacggcggcc
    10801 aaggcaaggc cggcgggcgg ccagatgggg acagcactcg agtagatcga ggcgagtgtc
    10861 aggccgactt tcgccagaac aaaatagctg gcgccgatga tcgccagctc gaaggcatat
    10921 ttcgccacgc cgcggggctg cagcgctgcg gcaaattttg atcctgaaaa gcgagtcacg
    10981 gcatgccccc actcaccgcg ccctctttcg taggtcctct cccgcaaatg cgcgctggtg
    11041 tgttccgata ctccccgact ttattgatca acggaactaa ggcggaactc gggccgtgga
    11101 ccggccaaac gagaccggcg ggaaccgaaa aatgccctgt gacgggctta aatcgccgct
    11161 cagatattag gcatggcagc ggtcacgcgt gcggccggga tatcgacgaa tcgacgcgcc
    11221 tttgaggcaa ctagtccaac ccacggcgct caaaaatagc ccaccgggcc ggcggcacgc
    11281 gcaagggcgc atcagccgct tcaacgcaag gccgaacgag aaagacgatc tcgcgcccgg
    11341 tccgaatcgg ccgggcggcg cctacatgga actctgcaag aattcaccga ccggcattgg
    11401 cggccgagga agttcgccaa aaatcctgtc gttttcgggc aagaaccagg acgggatttg
    11461 tctcagtctc gcgagcagcg gccgttcctc ctcgccgcta ttcgatgcag ctccgccata
    11521 cgcgactggc gcttggacac tttgccccgc cgccttaggc gcagaattgg atgcgacgcg
    11581 ggatggcgag atcgccagcg gctctcccga gcgcgccttc gcttccgcga gcgggccctg
    11641 ctcggacttt tgattgcggc gcggcagggc ggcctgcgcc ggttgcgcaa gcttcggcgc
    11701 ccagacggct gtcggcgtgg ccgccacgtt ttcctgcttg ttttcggttt ccgtccgcgc
    11761 ggtttcgccg acgagcgggc gatcgttttg ggtcatccgc gcgcgctcca cgctcgccgg
    11821 cgcgatctgc acgaccgggg cggaaggagg ttgcggcctt tcgatctggc cgaagatata
    11881 agcgcaggct gcgctcgcga tccccgcccc cgcgacctcg acaaatttga cagcgaatct
    11941 ggacagaaaa gacttggttc gcacgatccc ctccggtttc aaacgattga ctgattttca
    12001 aacacgcgtt tgagacaact tcagggcgaa aaggaaaggc aagcgtggca aagcagggga
    12061 aaagtgacgc ccgggcgcga ccgagcgatg gctgggcgcg cccggaaaac ttgcacgttg
    12121 aacgacttgc ggtacgacgg cgctttgccg aacgcgtcct aggaccgcgt cggcagtcgg
    12181 ccgagcccgt ctgcagccca ggtggcgatc gtgtcggcaa gtcggtccac gaagccttcg
    12241 tcgaccttga ggcgcagtgg aggattggca agctgctcgg cgttgcgaac gaaaacgctg
    12301 cactgcccaa acagccacaa agcgccgacg accgcctgct ggtccgtagc gttgttcggc
    12361 aggaaccggc gaaccaactc ggtcgcggcg gcaaagaaag gtgccgtctc gcgagccatg
    12421 aatttacgaa agaccggact cggcttgagt gtttcccagc tgaagatacg cacatattcg
    12481 ctgagctcgt cgcgagccgt catcggccgc agttgccggc gcacaaaccg gcgcaatgcc
    12541 gtttcgcgcg ggaggatgtc ggggttctgc ggcgcatcgt cgtccttcat cagcgcgcgc
    12601 aacgccgttt gcagaaccgc gcgataaagc ccttccttgc tgccgaaatg gtaattgatc
    12661 gcggcctggt tgacgtccgc ctcggcgacg atggctcgga tgctggcacc ctcgtagccg
    12721 ttcactgcga acgcgcggga cgccgctttg atgatcttgt cccgcgtggc cttcgagact
    12781 cgcgtcactt ttgctgtccg cggcatgccg attcccataa ttcaaacgac cgtttgaaca
    12841 taccgggcgg cgatcgcctc gacaagagcg cgccaggacg acccaagaat ctcacaaacg
    12901 ggagatagag acgcccgtga acgatgggcg tttgaccggc atgttggatt cacaggttcg
    12961 tgtcgtcgcc gatgagcaag tcgcgcagat tacagccgac aggaaagcca taccctcgat
    13021 atcgttcgaa ccacttcaga gaaccagcct tatgataggc acttgcatcg atctcggcac
    13081 cgatcatctg cagtgcgaga atcagtgcct gaaacgaatc gacgccccct gcgaaatacg
    13141 atcttctctc gtttggccag tcgatctcgt atcggcaaat ccacatgccc tcctcgtttt
    13201 ccggctggaa cagacgaact gatacttctg tgtcgcctga tgggccggtt acgatgagac
    13261 gtcgctcagc tactaccacg gctgcacctc ttggagtcct gctccaaacg atgccattaa
    13321 tttccgtgtc ttgcgaaccg tgtgacgatt gtcctgccgt gtggaaaggt cacagagcgc
    13381 cgccgattta gtgtgaggca attcggaaag cgcttattcc agctgcggtg gttcaaatcc
    13441 gggtacagtt caacgattgg attaatgaca tccaccaaca tgcatcccat agattgtccg
    13501 atcattcgtt tatatctttg aaattcatca tccgcgcaaa tggattgttc tcggcgagga
    13561 ccgcattttg ctccgctcca tgcttgatca tcaggtcgac aatcttcttc gccgcttcgc
    13621 gggacatcgg ctactcctgg acatcgactc ttgcctagcc tctgatagca tggcggacgc
    13681 ttacgcgcac ggatctgcgg ctaaaggatc gcagcagtcc catgccgttt gtctttgggc
    13741 ggcggtgccg tgggcgccgc ctttctcgtt ccaggctaag cccgtccagt ctctcggtcc
    13801 actcatgtac gtggttgtcg aaagtagtag cgggcacatc tttgggtttg ggttgacgcc
    13861 caagaaggcg gtggatcact tcctccgtgg tcttctgtcg tacgacgggc ctcgcctcga
    13921 cacccgccgc tgttcgcccg ccctctgccg ccagcttcaa gctcttgagc gcaggggtat
    13981 gttctttgtc gactgccgaa tcaaccggga cggcgttgca gtttgtcgaa aggacatccc
    14041 gcgggagcga gtgagcgtcc gaactgccaa gcaacgtcag cgaaagcgcc gtttaactcg
    14101 gccgcatccg agcctctcat aagagcgaag cagcgacata cacgcgagca gcgtggataa
    14161 ggctggcgtg acttgctcga acgccgcgtc taggatcggt aacatgttgg aagtagaggc
    14221 cccaagctca actgcggtct gagcagttcg attgcaaccc atgtacgtga tcgttggcga
    14281 cggacacatt tttggcttcg gactgactcc gaagaaagcg atggaacact ttctccacta
    14341 tctctggtat gacggacctc ccctcgacac gcgcccttgt tcgccggccc tctaccgaca
    14401 gcttcaagct ttagatcgca agggtctgcc ttttgtcgat tgtcgaatca accgcaaggg
    14461 cgtcgcagtg tgtcgaaagg acatgccgcg gacgattact cgtcgtaaag attgatctgt
    14521 cgatgtcgtg ggacgtcctc cgtggctccc ggccgcctgt acctccgtat tatatcgccc
    14581 cagtccagac gtttttgcgc attgcgcgcc gccacctccc cgaccagtct gttgtacagg
    14641 tcataggctt cctgctcggg gatgcgcaat ttcatgaatt ggtccggaga accgcccggc
    14701 gggtgctttt cgatcttgtc gattacatgc ttcagttcgt ggataccaac ttttctggcc
    14761 cattccaaac ttggcgcatg gactgccacc cagtttctgt tgacgtcggt cgcgccgcgt
    14821 ggattttcga gtggatcgat ggtcaagcgc gactgccaat ttgcaaggcc aggataggcc
    14881 tcctgcatgc ccggatgagc gtgatgttcg taaaggggtg caacggtgag cgattgcgtc
    14941 cctattcgtt cgcctgcatt cggattgacc tgatatccct tgtccggaat ttcgaaggtc
    15001 cagcgctcat ccgccgcccg ctccaaacca gtcatgcgcc aaatctcatc ggccgatttg
    15061 cccgcggcct ccatcttcat tgcggtgggc agcttgtccc aaggaaatgt tctcgccatc
    15121 gtgccgccga tgatcgcgag cttggtgggc ggaagccagt cgagagctgc attgacggtg
    15181 ccgagaccca aatgagctgc cgccttgccg taatttccgg cctttgcctc ttcaccagca
    15241 agcgatgcgt cctgagttga ctgcaccgtg ccggagcccg gcaacagctg agtgagcgcg
    15301 tgcaacttca gcagcgcgtc atagaggtgt gggccggcgt aatagcggac attttcgtcc
    15361 gacagggcgt cacgcaacca cgtccgaaaa tccttcggtg ccgggggcgg tgccggctcg
    15421 ggaggaggca gcagaacatc gcctggacca taaaacggcg gactcggttt agttgacgac
    15481 ccctgcggaa cggttggcgt cgtcacactt gtctcctcag gcggcagacc gaccgaaggg
    15541 ctctcaatcg tctgggcggc tatcccagga agcccaaaac ccccaagggg aaacgtattc
    15601 cacgagaacg tgctgttttg actccgactg acttccggta gtggggacca gaatacgggc
    15661 gagtttgtcg gcgtcgccgg aggccacgcc atcactgccc atgggttggc ccacaggtcc
    15721 tcaatcggcc gggtgaggtt tccgagaatt ccaccacgcg aggtttccgg ccacggggca
    15781 tcccaagtgt ctcttggcgg cgtcggcggc gtgcgagcgc ccaacagcga gttcggcatc
    15841 cagtaaggcc aagtctccgg ccgcatgaac cagctagggg gcggaaattg atcattcatt
    15901 ggagttttct ccctgttgcg agcgtggctt gccggaaacg aacgtgcgag cttggccgca
    15961 ggcttcgtcc agcgaacatg cgtgagccaa ggtctgcatt gcactgtggc cagctgggcc
    16021 agcggcgaga tacgacgtag cgaatttcag aaggcgacct tcggcagcaa ttggctgcca
    16081 accgggcaag ccaggaggcc tagcggcacc ggatgggtcc accggttccc tagcgcttgc
    16141 tccaaactcg cgaactgccg cgaggtgttg gaattgatcg tgacttgaac gcgacaggcg
    16201 gcgcctatcg gttcaaaaac ctgttctgag atttgcggac gcccgacccg ccgaggctca
    16261 gccggcctcg aaacaggtgc tcacgggcgc atgtttggtg aatacgcttt tatgaaagat
    16321 tcgtgtaacg cgagtccagg gaaagaaatg aatttcgctt cgctcggcgc gcgagccata
    16381 ggctgatgcc tttaaccgcc taaaacagct cgaaatactc ccgctgctcc cactccgtga
    16441 cttccgagtg gtagcgggcg atctcagcct ccttgatacg caggtaatag tcgatgaaat
    16501 ttttaccgaa gccggcggcg aggcaagcat cctcgcgcag cgcggcgagc gcctgctcga
    16561 ggcttttggg caaatgcggc gcctcgactt cgtacggcgc gtccgccgac gggccgggat
    16621 cgagcattcg ctccatgccg tcgaggccgg tgacgacttg cgacgccatg tagagatacg
    16681 gattcgcggc cggctcgccc acccgattct cgagccgcgt ggcggcgtcg ccagggccgc
    16741 cgagcacgcg gatcatcgcg ccgcgattgt cacgccccca aatcacgcgg tccggcgcca
    16801 gcgaatagcc gcgatagcgc ttgtagccgt tgatcgtcgg ggtggtgaag gcggcggccg
    16861 cgtgcgcgtg ggcgagcagg ccggccaaaa agctccgccc cgcgcgcgac agcccgtcgt
    16921 cgtcgttcga catgaaaacg ttggcgctgg agcgccggtc gcgcaaggat tgatgcaggt
    16981 gccagccgct cgacatcacg ttctcgacct tcggacgggt catgaaggtg gcgtggcagc
    17041 cctgccgccg gcaaatctgt ttcgcggcgc tgcggaacag catcatggtg tcggcgggtg
    17101 agagtccgac ctgcggctgg aacgtgaact cgcactggct cggaccgagc tcgacctcga
    17161 ccgagcgcaa tggcaagccg agcgcgacga cctcgcggcg cagcgtttcg agcgtgggct
    17221 cgaactgatc gaagcgcgtc tcggtcaaat attggtagcc ctgggacagc aagctcaccc
    17281 gcggcgcctc gcccggccag gtggcatcgg ccggcatcag gcgcggatcg tcgagcttga
    17341 agacatggaa ctcgacttcg agcccggcga cgtagtcgaa gccagcctcg gcaagccgtt
    17401 gcaggacgcg gcgataaaga tgccgcgtcg agaacggcac cggctggccg gtggcgaaat
    17461 agatgtcgca cagtacccag cccgtccgcg gcgcccacgg cagcaagcga aaggtcaagg
    17521 gatcggcgat catcaagaag tcgccgccac cctccatctc ggtcatgccg aatccgccgc
    17581 cgggggtgaa aaccggaaag gccgtcttgt gcgcggtgtc cttggcaagc agcgtggtgg
    17641 tgatcgagca accgttgcgc atggcggccg cggcctcgtt cgccaccatg gtttttccgc
    17701 gcagaatgcc gtgctggtcg ggaaacgata ggcggacgac ctcgagcccc tgttcctcga
    17761 tccatttatc ggcgcgggcc gcggcctcga actgctcgtc gctccacaag ccgtggcgct
    17821 cgacgaagct gcgcgccgtt ctttcctgcg atgacgacac cgggccaccc aaccgctgat
    17881 cggcgaaggt tagagtattt atgtgcgcgc ttcgagaggc gccgagcgcg gcgttcaatg
    17941 tacgcttgcg gccttcgcct gcaggctcgc gatgagatcg ctcgacccct gcaccgccaa
    18001 gcgctgatga taaaagcgca atccgtaaag gccgcccagc tcctcacccg ctccggcgcg
    18061 ccccgggccg ccgtggttgc attgcggcat gacgataccg tgcccggtat gagcggtggc
    18121 gatcgcggga tcgacggcga gaatgcggcc atggctcggc gcg
//
LOCUS       DATLJT010000892         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2125954,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000892 DATLJT010000000
VERSION     DATLJT010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125954"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..295)
                     /locus_tag="VM910_20615"
     CDS             complement(80..295)
                     /locus_tag="VM910_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64962.1"
                     /translation="MPSPSSQSQHTPSLFEASRVKQAVFSVGRARHSGGEAGEIGGEI
                     AQALAAKRPVIIDVVTDIDALAPRAVA"
     gene            551..910
                     /locus_tag="VM910_20620"
     CDS             551..910
                     /locus_tag="VM910_20620"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HVI64963.1"
                     /translation="MGAGRQKAYNCMVFAQNDPHDVEVAMKYVLLGNLSPEYASKQSE
                     RVRKAKAKLDKLGIKIESIHYTQGYYDFVDIVDAPTPEAMLAFSVWYSTQRLGRIQSM
                     PAFDAKSFETAIKDAAG"
     gene            1881..2327
                     /locus_tag="VM910_20625"
     CDS             1881..2327
                     /locus_tag="VM910_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64964.1"
                     /translation="MRSAPCHFAALLPLVVLLAAGVPALAADSIEELLHQHEADDDND
                     ALRPDLNALTEEQWQYYFKVERKQPDIELQLIVRESEERPRVLYIWVAAIAAGFGMAI
                     GAIQASHYLSCRRLCRTTVATADTSHILPSAPANIQSATTQRLGAS"
     gene            complement(2991..>3236)
                     /locus_tag="VM910_20630"
     CDS             complement(2991..>3236)
                     /locus_tag="VM910_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64965.1"
                     /translation="VVMGGSVTGLSFQQTDLAGKRLELLHEVVPVLRRSAILANAGNP
                     VTLADMRKVEAAVGLLGLTVVTLEIRRMEDIARFRGA"
BASE COUNT          669 a          972 c          971 g          624 t
ORIGIN      
        1 gagattctat ctctgctcaa atgaagcttg atccgacgct ggttggggac cgtccgcttt
       61 cagccgtatc agtcgccgct cacgccaccg cccgcggcgc gagcgcgtcg atgtcggtga
      121 cgacgtcgat aatcaccggc cgcttcgccg cgagcgcctg cgcgatctcg ccgccgatct
      181 cgccggcttc tccacccgaa tgccgagcgc gcccgacaga aaaaactgcc tgtttgacgc
      241 gcgacgcttc gaacagacta ggtgtatgct gggattggct tgatggagat ggcaatggcg
      301 tcacgaggaa gcccggggcc gcgcccgcgg cagggagacc actgcactac gggatttcga
      361 tccggccttt gtccgcttcg ggtcattcgc gtcttgagcg gcctcgcccg atgtccgctt
      421 cgccccgaaa gcggacaaac agtagatcgt ctcgctaagt ccgcttggtg ccaatcgcgt
      481 tctagcgcgc cgcagcaaag acggcttcgc ccatggtgtg gtgatgcggg tagcggtaat
      541 gcgaaacggc atgggcgctg gtcgccagaa ggcgtataat tgcatggtct tcgcgcaaaa
      601 cgatccacat gacgtggagg tggccatgaa atatgtgctg ctcggaaatc tgagcccgga
      661 gtacgcaagc aagcagtcgg agcgagtcag gaaggccaag gcaaagctcg acaaactggg
      721 catcaagata gagtcgatcc actacacgca gggctactac gatttcgtcg acatcgtcga
      781 tgccccgact ccggaggcga tgctcgcgtt ctccgtctgg tactccaccc agcgtctggg
      841 acggatccag agtatgccgg ccttcgacgc aaagagcttc gaaacagcga tcaaggacgc
      901 agcgggctga gcgcggggtg gctcacagct ttgctttgtg gtgaccgcca ttgatggacg
      961 agcgctggag aaatcgatca cctcgtcagc gagcgcgagc agcgagatgg gctggtcgag
     1021 gccgagcgcc tcggccgtcg tcaccccaaa tacaacagcc gttgcctcga ctgatccgcc
     1081 cacggggaga taggtgccct cattgatgcg gcgcggatcc gtggatcggc gaaccatcct
     1141 tgcgattagt ccgatgccgc cggcattcgg caaatgtatc attcatttca gcgctgcttt
     1201 gtcgcagtcg tgcaagcatt accgacacaa cagctcgaaa gcgttccctg ggcgcctcgg
     1261 gtggtaatcc agtttccacc tattgccagg cgacgacatt tgggcgtctc gcattttttt
     1321 cttgcgtgag ccacgtctgt cgcttcccaa actaccgctg ccgtgagcgc cagcacagct
     1381 agtgatatgg tccaaagccg atacatgtgg aatctcctac taacccgttg atgtcgcgcg
     1441 atccggcatt cccttcacac gagatcgctc tccccttaca accttcaggg ctcgctcggg
     1501 ctgcgcgcgc gccgcgagcg gccacgcagc cgcgccgccg aaagccaaga tgaactcgcg
     1561 ccgtctcatt cgatgacctc gtcggcgcgg gcgggcgatg tcggcccggc ccgcaagaag
     1621 agggcagcac gggggtggtg cacgaacgcc gactggcgcg caggtcgcat ctgcaccgcc
     1681 ataccgaagc catttcaagg gattgtggtt gcgttttaac ttgttggtaa ccacgcgccg
     1741 agcgtgacgg ttgacggtga aggctcagag atcaagccta agggacggaa gtgccgtaac
     1801 actctgctaa tggaacagct gtaggggcag gctgaatggt gacctcagca caatgcaggc
     1861 cgcatcggcg gcaagaagac ttgcgatcgg cgccgtgtca cttcgccgca ctgctgccgc
     1921 tcgtggtgtt gttagcagcg ggcgtacctg cgctagccgc tgattcaatc gaagagttgc
     1981 tgcatcagca tgaggccgac gatgacaacg acgcgctaag gccagacttg aatgcgctca
     2041 cggaagagca atggcaatac tacttcaagg tcgagcgcaa gcaaccagac atagagcttc
     2101 aactgattgt gcgtgagagc gaggagcgcc cgcgcgttct ctatatttgg gtcgcggcta
     2161 tagctgcggg ctttggcatg gcgatcggcg caatccaggc ttcgcactac ttgtcatgcc
     2221 ggcggttgtg taggacgacg gtcgccactg ccgacacttc tcacatactt ccgtccgcgc
     2281 cggcgaatat tcagtctgcc acaacgcagc ggctgggcgc gtcgtgaccc accgatcaac
     2341 gcatggcttg ccgctggttt ggtgcccgcc gatacacaga gagtaaaaga aagccccgga
     2401 cgcgccgggc tttttcaaga gaagttatgc gaacatctag ggatcaaggt gccctagacg
     2461 cgccgatact atctcagtca cgcaaggcgc agaattgcta tctcgctatg gcagatgcgc
     2521 cccgacccgc cgctggatac gacgggccgg gacttgtcat ctgcaccgat gagtgcgggt
     2581 acggcgcgga tgacaactga gcaacgccgc acttgctgcc tggttcggtg cccgcagata
     2641 cacagcgaga caaagaaagc cccgggcacg gggtccgggg cttccaagga gaactaatgc
     2701 gaacatctag ggatcaaggt gccctagacc ctccgatact atctcagtca cgcaaggcgc
     2761 agaattgcta tctcgctatg gcagcgatgc gccgatggtt cctcacgcgg gctgcgcccc
     2821 tatgctggcg tgcggctcgc ctgcctccta tctccgatag gtgcgcagcg tcacaccgaa
     2881 caagccgttc agttttttgg aatcgtatgt cgatctgtct gttgcacgcc gagcgtcagc
     2941 atttggtgcg tgtttaggat cggatcgggc gaaacatatc cgcgcgagcc ttatgcgcct
     3001 cgaaaccgag cgatatcctc catacgtcga atttccaacg tgaccaccgt gaggccgagc
     3061 aggccgaccg ctgcctcaac cttacgcata tccgccaggg tgacaggatt gccagcattg
     3121 gccaaaatcg ctgaccggcg aagaacaggg acaacctcgt gcaaaagttc gaggcgtttg
     3181 ccggcaagat cggtctgctg gaacgataag cccgtgacac tgccacccat cacgac
//
LOCUS       DATLJT010000893         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1437256,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000893 DATLJT010000000
VERSION     DATLJT010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437256"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VM910_20635"
     CDS             complement(<1..563)
                     /locus_tag="VM910_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI64966.1"
                     /translation="MSSQSKTSATHEALRASEQQFQLLVQGVTDYAIFMLDPQGRITT
                     WNSGAERIKGYHAEEIIGEHFSRFYIEEEQREGVPMRALLRATMEGRYEAEGWRVRKD
                     GSRFRASVVIDPIHDDAAKLIGFAKVTRDVTERHSAQEMLEQARERLLQWQKMEAIGQ
                     LSGGVAHDFNNLLTIVIGNLETAQRQLG"
     gene            complement(945..1427)
                     /locus_tag="VM910_20640"
     CDS             complement(945..1427)
                     /locus_tag="VM910_20640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI64967.1"
                     /translation="MTELSARKRFGVIDDDQQRAMSGLEYVQGLVDGTLPLNTMAQTL
                     GYDVVEVSKGRVVAAAEPHAGHLNPAGTVHGGLAATLLDTCMGLAIRSMLDKGFAQTT
                     LEFKISLVRPITPQTGRVKAEGKVISCGRRVGTAEGTLTDKDGRVLAHGTTTCLIFEQ
                     "
     gene            1698..>2852
                     /locus_tag="VM910_20645"
     CDS             1698..>2852
                     /locus_tag="VM910_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVI64968.1"
                     /translation="MHPGVAPYWKRAGIRLIALLAVVAAIGLSLTDRTSAAQRVAKSA
                     PAAIAPKPPNGKDRAVARPPSAPPANASSQREEKNGRQQAAQGGAKTNTIARNGLPRR
                     PLPGEAGFTGVPPRGERRFISNEMVFHSGANVSPQAVEAAARRLGLTPLGAQSLTLTG
                     GRLFRFRLGEGRQVADAVRALEAEKLGTAQPNYVYSLQQNAASAPTLPAGDAAQYAVI
                     KLRLRDVHRWSVGKDVPVAVIDSQIDAAHPDLAGAFGEQFDAVGKADKADNHGTGMTG
                     AIVAHRRLLGVAPGVRILAVHAFSPDQQNSAQATTQHVLAGIDWAIEKGARIINMSFA
                     GPYDPMLQLALKKAHDKGIVLIAAAGNAGPRSQPLYPAADENVIAVTAIDA"
BASE COUNT          530 a          911 c          899 g          512 t
ORIGIN      
        1 ccgagctggc gctgcgcggt ttcaagattg ccgatgacga tggtgagcaa attgttgaaa
       61 tcgtgtgcca ctccacccga gagctggccg atggcttcca tcttctgcca ttgcagcagc
      121 cgctcgcgcg cttgctcgag catttcctgg gccgagtgcc gttcggtgac gtcgcgcgtc
      181 accttggcaa agccgatcag cttagccgca tcgtcgtgga tcggatcgat cacgacgctt
      241 gcccggaacc ggctgccatc cttgcgcacg cgccagccct cggcctcgta acggccttcc
      301 atggtcgcgc gcagcagcgc gcgcatcggc acgccttcgc gttgctcctc ctcgatgtag
      361 aagcgtgaga agtgctcgcc gatgatctct tcggcgtgat accccttgat gcgttcggcg
      421 ccgctgttcc aagtcgtgat tcgtccctgc ggatcgagca tgaagatcgc gtagtcggtg
      481 acgccttgca ccagcagctg gaattgctgc tcgcttgctc gcaatgcctc gtgcgtggca
      541 ctggttttgg actgtgagga catggggcga ttgacgattc ggggtgactg acgattcaaa
      601 ggaggatgcg gcgcgacgca aacggcgcga cgggcgcaaa cgccgccgca tcgatcggca
      661 tgcgtggctt acttgaagcg gtgcgaagaa atttcccgtg ggtcatggcg gctcccgccg
      721 acaaacgtga gcacgcctta ccagtccaaa attgaaactc tgaaaacttg acccgggttc
      781 cgctcgagag ctgaggggac gcgggcgaat gattgcaaaa gtgaaggaac cttttcgttg
      841 caagcgtgtt aggggccgac gcgccaacgg atctcactca tggtgatcgc gtcgcgcgtc
      901 cttgtgcgcg ggcatgtccg tatctgaaac gccggcgact tgctctactg ctcaaaaatc
      961 aagcaagttg tcgtgccgtg cgcgagaacg cggccgtctt tgtcggttaa cgtcccctct
     1021 gccgtaccga cacggcgtcc gcaagaaatc actttgcctt cagccttcac gcggccggtc
     1081 tgcggggtga taggccgaac cagcgaaatc ttgaactcga gcgttgtttg cgcaaagccc
     1141 ttgtccagca tggaccggat ggcgaggccc atgcaggtat cgagcaaagt cgcggcaagg
     1201 ccgccatgaa ccgtgccggc ggggttgaga tgcccggcat ggggttctgc ggcggcaaca
     1261 acgcggccct tggaaacctc gacaacgtcg tagccgagcg tttgcgccat cgtgttcagg
     1321 ggcaacgtgc cgtcgacgag cccctgaaca tattcgaggc cgctcattgc ccgttgctgg
     1381 tcgtcgtcga tgacgccgaa gcgcttgcga gcagagagtt ccgtcatgaa ttccaccggg
     1441 agctagtttg ggtgccacac ccacgatcgg atcgcgccga tcgacgtccg atcgcaagcg
     1501 agcccgggcg atggccaata agagcatcta ccgattcgag gagcaaacgc acaaacaccc
     1561 cgcgcatggc ggcttgtaag gatcgcaggt caccaagtct gaaacactcc ggttcttgcc
     1621 gcggaattgg ctgccccgtt gacgcaaagt cggcgcaatc ccgctctatc ggttgatggt
     1681 cgattgaggg acgcttcatg catcccggcg tagcgccata ttggaagcga gcggggatac
     1741 ggcttattgc cctgcttgcc gtcgtcgccg cgatcgggct gagtctcacg gatcgcacgt
     1801 cggcggctca acgtgtcgcc aagtcggccc cggcggcgat cgcgcccaag ccgccgaatg
     1861 ggaaggaccg cgcggtcgct cggccgccgt ccgctccgcc ggcgaacgcc tccagccagc
     1921 gcgaggaaaa aaacgggcgc cagcaagccg cccaaggcgg cgcgaaaacg aacaccatcg
     1981 cccgcaacgg actgccgcgt cggccgcttc ccggcgaggc cggcttcacc ggcgtgccgc
     2041 cgcgcggcga acggcgtttc atctccaacg aaatggtttt tcacagcggc gccaatgtat
     2101 cgccgcaagc ggtcgaggcc gccgcgcgcc ggctcgggct cacgccgctc ggcgctcaga
     2161 gcttgacgct caccggcgga cggttgtttc gctttcgcct cggcgaagga cggcaggtcg
     2221 ccgacgcggt gcgcgcgctt gaagccgaaa agctcggcac cgcgcagccc aactatgtct
     2281 atagcctgca gcaaaacgca gcttccgcgc cgacgctacc ggcgggcgac gcagcccagt
     2341 acgcggtcat caagttacgg ctgcgtgacg tgcatcgctg gagcgtcgga aaagatgtac
     2401 cggtcgccgt catcgattcg caaatcgatg ccgcacatcc cgatctcgct ggcgcgttcg
     2461 gcgagcaatt cgatgccgtt ggcaaagcgg acaaggccga taatcacggc accgggatga
     2521 ccggtgccat cgtcgcgcat cgaaggcttc tcggcgtcgc gccgggcgtg cgcatcctcg
     2581 ccgtgcatgc gttcagcccg gaccagcaga attcggcgca ggccacgacg cagcacgttc
     2641 tcgccggcat cgattgggcg atcgaaaaag gcgcccgcat catcaatatg agctttgccg
     2701 ggccctacga cccgatgctc cagctggccc tcaagaaggc gcacgacaaa ggcatcgtgt
     2761 tgatcgccgc cgccggcaat gcggggccgc gctcgcaacc gctctatccc gccgcggacg
     2821 agaacgtgat cgcggtgacg gcgatcgacg cc
//
LOCUS       DATLJT010000894         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2355618,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000894 DATLJT010000000
VERSION     DATLJT010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2355618"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VM910_20650"
     CDS             complement(<1..592)
                     /locus_tag="VM910_20650"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HVI64969.1"
                     /translation="MTDQSTRWTEPLARVLAPADDTPRDVMGSILLNLAVFVAYSIAG
                     LVVLVFAIGLAKISPIYPPAGIAVAASFIFGPRILPAVFFGQFLNGFPLLAEPQMTLA
                     RFVFANTGGGVAAVLEASISVALLRQLAGAWHPFERPRDVVIFLVGSCLVAALVGGMV
                     GTLSLWAAGIVPTEQLQITFVTYFLADAAGIAVFGSL"
     gene            complement(818..2032)
                     /locus_tag="VM910_20655"
     CDS             complement(818..2032)
                     /locus_tag="VM910_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HVI64970.1"
                     /translation="MAFDYAQISGPKRRAKVLLRTRNLKMARNAHAYVRGSTVKFYEW
                     LEDGGRKLPAGPSVWICGDCHLGNLGPLADADGHVDVQIRDLDQTVIGNPVHDLVRLA
                     LSLASAARGSDLPGVTTARMLEEMMNGYEAALAGRFGGQTEKNHRPKLIREILDRAVR
                     RKWRQLAEERIENVKPSIPLGKRFWALTKSERKALKELLKSDAARELITALKGRREDD
                     RIEVLDAAYWMKGCSSLGRLRYAVLVGVADGAPQSNNLCLIDIKEGVLGAAPRAPDAK
                     MPRDNAERVVAGARALSPNLGERMMAGRLFDKSVTVRELMPQDLKIEIEQLSREQATA
                     IAGYLAGVVGRAHARQMDGATRRSWKSELARNRSKTLDAPSWLWLSVVELMGSHEAAY
                     LDHCRRYAWDEA"
     gene            complement(2199..2456)
                     /locus_tag="VM910_20660"
     CDS             complement(2199..2456)
                     /locus_tag="VM910_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64971.1"
                     /translation="MIAPTLERAPRMLLVIGEDEQGLPTETIILEPSVSRLYDALRAW
                     LEVVLPNWLGRALRLTRPSHVGPLSASEPVLIDVQLRWAGL"
BASE COUNT          505 a          848 c          762 g          453 t
ORIGIN      
        1 tgagagatcc gaacaccgca atgcctgcgg catccgcaag aaagtacgtc acgaacgtga
       61 tttgcaattg ctctgtcggg acaatgccgg ccgcccagag cgacaatgtc ccgaccatgc
      121 cgccgacgag tgcggcgacg agacacgagc caaccaggaa aatcacgaca tcgcgcgggc
      181 gctcgaacgg atgccacgcg ccggcaagtt gccgcagcaa cgcgaccgat atcgacgctt
      241 ccaggactgc tgcgacaccg ccgccggtgt tggcgaagac gaacctcgcc agcgtcatct
      301 gaggctccgc cagcagcggg aaaccgttca gaaactggcc aaaaaagacc gccggcagga
      361 tccgtggtcc gaagataaag cttgccgcca ccgcgatgcc cgcgggcgga taaatcggac
      421 tgatctttgc gaggcctatg gcgaaaacca gcaccaccag cccggcgatg ctgtaggcca
      481 caaacacggc caggttaaga agaatagaac ccatgacatc gcgcggcgtg tcatcagccg
      541 gcgccaacac gcgtgcgagc ggttcggtcc accgtgtcga ttgatccgtc attgatacaa
      601 ccgggcgatc tccgagctac ctccataccc ggtttcgacg tcattgccag ctggcattcc
      661 ggcgtcagcc gcgatcccgc caatggatgg ctgcgagagc ccttggcgac cgtcgcgaaa
      721 cgatgagaaa gacgcgctga gttggccgct cagcggcgtg aggtcgcagg cggccgcggc
      781 tgcaatcttt tgactgcagt cggcaaacgc agacgtttca ggcttcgtcc caagcgtagc
      841 ggcggcaatg atcgaggtaa gcggcctcat gcgaccccat cagctcgacc acgctcagcc
      901 aaagccatga cggcgcatcg agcgtcttcg accggttgcg ggccaattcc gatttccagc
      961 ttcgacgcgt cgctccatcc atctgacgcg catgcgcacg cccgacgacg cccgccaagt
     1021 accctgcgat cgccgttgcc tgctcccggc tgagctgctc gatctcgatc ttgaggtctt
     1081 gcggcatcaa ctcccgcacg gtgacgcttt tgtcgaacag ccggccggcc atcatccgtt
     1141 cgcccaaatt cggcgaaagc gcgcgcgccc ccgcgaccac gcgctcggcg ttgtcgcgcg
     1201 gcatcttcgc atcgggcgcc cgcggcgccg cgcccaggac gccctccttg atgtcgatga
     1261 ggcagagatt gttcgattga ggcgcgccgt cggcgacgcc gacgagcacg gcgtagcgca
     1321 gacggccgag cgagctgcaa cctttcatcc aataggcggc gtccagcact tcgatcctgt
     1381 catcctcacg acggcccttg agcgcggtga tgagctcgcg ggcagcgtcg ctcttcagca
     1441 gttccttgag ggctttccgc tcgcttttcg tgagcgccca gaatcgcttt ccgagcggga
     1501 tcgaaggctt cacgttctcg atccgctctt cagccagttg ccgccacttg cggcgaaccg
     1561 cgcggtcgag gatctcgcgg atcagtttcg gccggtggtt cttttccgtt tggccaccga
     1621 atcttccggc caaagcggct tcgtagccgt tcatcatctc ttcaagcatc ctggccgtcg
     1681 tcacgccagg caaatcggac ccgcgagccg ctgacgccaa cgacagcgcc agccgcacga
     1741 ggtcgtggac gggatttccg atgaccgtct gatcgagatc gcgaatttga acgtcgacgt
     1801 ggccgtcggc gtcggcgaga ggtcccagat ttccgagatg gcaatcgccg cagatccata
     1861 ccgacggccc cgccggcagt ttccggcctc catcttccag ccactcgtaa aacttcaccg
     1921 tacttccgcg cacgtaggcg tgcgcgttgc gcgccatttt caaattgcgc gtccgcagca
     1981 gaactttcgc gcgccgcttg ggaccggaaa tttgcgcgta gtcgaatgcc atgggccacc
     2041 aaactacagt gcgggaaggg aaattgaata aggcgatcgc gctgagcgcg caagcgcaat
     2101 cgcgcgccgt ggcaaagccg ggctcatgtc ccgcaacgac aacgggtacg gggcggatgc
     2161 tgcatcccct accaccgact tgctccaggc tcggctactt atagaccagc ccatctcagc
     2221 tggacgtcaa tcaacaccgg ctcggaagcc gaaagcgggc cgacatggct gggccgcgtc
     2281 aggcgtagcg cgcggccaag ccagttcggc agcacgactt ccagccacgc gcgcagcgca
     2341 tcatagagcc gtgacacgct cggctcgagg atgatggtct ccgtcggcag accctgctcg
     2401 tcctcgccga tcacaagcag cattcgcggc gcgcgctcga gggtcggcgc gatcatgatg
     2461 gtctcgcacg caatggggtg acaaaatcta cctttgtagt tttaatttcg atcgtcaatg
     2521 cgacgagcgg ccaaagcgcc ttccttcgtt tgaaaaacct gaggtgtg
//
LOCUS       DATLJT010000895         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_58977,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000895 DATLJT010000000
VERSION     DATLJT010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58977"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VM910_20665"
     CDS             complement(<1..118)
                     /locus_tag="VM910_20665"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HVI64972.1"
                     /translation="MRTGLTTAALTLIPTAAFAHPGIGDAHGFVAGFAHPLGG"
     gene            1529..2182
                     /locus_tag="VM910_20670"
     CDS             1529..2182
                     /locus_tag="VM910_20670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI64973.1"
                     /translation="MHAARHSSNAHAANRGAMPYPALFKRLKRRKPLRLRCERCGRTC
                     VEEKPAPRSAGIEIITSQAHNKVAAVAAAIEDARPLSMRINAWEWRWPLNTYRERDAN
                     KLSRAMLDRLAEAERMTLDQNREDLAHRDEVRARYAELEADCDACISLSAPAAAPLGA
                     GSTGDPNCTVHASLLGIPAISLPMLQDDGLPLGLQIAGFVNGDAQTFAIAAAIKPLL"
     gene            complement(2710..>3276)
                     /locus_tag="VM910_20675"
     CDS             complement(2710..>3276)
                     /locus_tag="VM910_20675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI64974.1"
                     /translation="QRVNRAGTHLLGLINQVLDLSKIEAGKLELNPQTVQLAPLINEV
                     IGTAGQLAEQNENRLVVDAQENLGALTVDAMRLRQILLNLLSNACKFTKTGEVKLAAR
                     KVSNGSNFVEFAVSDTGIGMTAEQQAKLFEEFSQADATTAQRFGGTGLGLALSRKLAR
                     MMGGDVTVTSEPGKGSVFTLRLPGGAEH"
BASE COUNT          627 a         1044 c          995 g          610 t
ORIGIN      
        1 gcccgccgag cggatgcgcg aagccggcaa cgaaaccgtg cgcgtcgccg atgccgggat
       61 gagcgaaggc ggcggtggga atgagcgtga gggcggcggt cgtcaagcca gtgcgcatgg
      121 tcgttccctg ctatcggtcc aaaaaaatga tcgacgcaaa atcgcacatc agcggcgtgg
      181 tccgcaaggg cgcgccgccc gtcgctgaca tctgcaagcg gatcgatggc cgacgcgccg
      241 atcgcggcgt gcctgtctgc gtcgtcacga gaacgtcgct gcgcgggtat gaggcagaaa
      301 aaacaggagc aggtccgccc ggatggagac atccggactc gaggctctta gccgttgtcg
      361 gcggttgtca gatttttcga agaaagcctt gcggcacggg cacttcttgc atggctcaca
      421 aaaattccgt aagcgcatca ataaaggtct ttttgcaacc aatcgccccc taaatcgccc
      481 ccaaatcccg ttcgacaccg tgagcccgtc ggtgcgcaat tctgacctcg gaggcaaccg
      541 gcataggaac cgcgaccgcg gcgcgatcct aggcatcacc agcgagtgct agccgatcgc
      601 gaatgggcgc cgagccgtct taagaccacg gccctgaagg aacggcatag gcacgggctt
      661 catttgcccc tgtccccatg cctctggcgg ctcgctgcct ctcgacttcc gcggcgccgg
      721 ccgattattc gctttacgca atgttgcgta ccacctaccg ccctcccgct tcatcgagcc
      781 ctgcctgcca tctcccgccg agcgaccacc gagcggtccc ggctggattc acgagatcaa
      841 cggattccgg ctgatggcgc ggcgtgacgc cgctgatgtg cggctgctga agctcggcgg
      901 gctgatggct tacggccctc attttccttc gatgtttacg cgcggggcga gctacgtcgc
      961 tcgcattctt gcgggcgcca aggcaagcga gctacccatc gagcgaccaa gcaagtttga
     1021 gctggtgatc aatctcaaga cagccacgca agttttcccc gcgcgaccct ttcccgccat
     1081 cgccactgtg caccgtcctg cacttaccgt gacgctcgtc gccatgcccg gcgtcggcgg
     1141 cataaaaagc tttcgtccga cggtccgaat cgcacaatgc cgcttaactc gaattttcca
     1201 gcaaagatcg gaccgaaaaa gagggagacg gagtcacagt gtctcactat ccggacgact
     1261 gattgggtgc gccactcgaa atttctgcca ttggaggcta gcctgatttc gcgcgccttg
     1321 gcggccgcga accgcatttt cgtccttaac tgggcgccac aggcggtcct ggccagtcgc
     1381 gatgctttcc ggaggggggc atcagaaaat tctcgagccc ccgaaacacg gggcgcggcg
     1441 gcgcgcaagt ttttgaacct gacaaaacat tttttgaagt tgatccggca cgtgacatgg
     1501 tgtccgcccg gacatgggac ggcaccggat gcacgcggca cggcacagtt ccaatgccca
     1561 tgcggccaac cgtggggcaa tgccttaccc ggcgctattc aaacggctga aacgacgcaa
     1621 accgctacgg ctgcggtgcg agcgctgcgg ccggacctgc gttgaagaaa aacccgcgcc
     1681 acgctccgcc gggatcgaga tcataacaag ccaggcgcat aacaaggtcg ccgccgtcgc
     1741 cgctgcgatc gaagacgcgc gtccactctc catgcgcatc aatgcgtggg aatggcgctg
     1801 gcctctcaac acttatcgcg agcgagacgc gaataagctg agccgggcca tgctcgaccg
     1861 gcttgccgag gccgagcgca tgaccctcga tcaaaatcgg gaagatcttg ctcaccgcga
     1921 cgaagtgcgc gcgaggtacg cggagctcga agccgattgc gacgcttgca tcagtttgtc
     1981 ggcgccggca gccgcgccgt taggcgcggg ctcgaccggc gatcccaatt gtacggtaca
     2041 tgcctcattg ctcggcatcc ccgcgatctc gctgcccatg ctgcaggatg acggtctgcc
     2101 cctcgggctg cagatcgccg gtttcgtcaa cggggacgcc cagaccttcg ccattgcggc
     2161 cgcgatcaag ccgttgctct agcgtgagcg caggcgagcg gaggcaggca ggcaagcgca
     2221 ggcaggagcc tattcttgcg tgctcgcgct gttcagcagg ttaccgatcc tgccacgagc
     2281 tcggtggata tgccggcgag agcgtcggca acgatcgcct tacgcttctc gctcagttcg
     2341 aacgcgacga ctagcggtgg ccgactcaac tccatgcatc cccaagatta ccgcaactcg
     2401 gctcagccac cgctgatgcg gccattccgg tggtggcaac cttgagaaat tcgcagcgtt
     2461 tcacccatat ccatggcaca caccgcgccg gctggaggag ccggccacag catcaggagc
     2521 ggacgttcca cgccggcacc ctcaacaggt ggggacactt gtcttggaaa agcgcttagt
     2581 tctcaatgtg cccagagagt gcctattggg gacaccttgc actaaaaaat gtaaagatat
     2641 caacaaacgg ctcgatccgt tctgaatcga gacggggtgc tgccggccgc ttcccttctg
     2701 agcctttaat cagtgctccg cgccgcccgg caggcgaagg gtgaagaccg agcccttgcc
     2761 cggctcgctt gtgacggtca cgtcgccgcc catcatccgc gcgagcttgc gcgagagggc
     2821 aaggccgagc ccggtgccgc cgaagcgctg cgccgtggtg gcatcggcct ggctgaactc
     2881 ctcgaatagc ttggcctgct gctccgcagt catgccgatg ccggtgtcgg agacggcaaa
     2941 ctcgacaaag ttgctgccat tgctcacctt gcgtgccgca agcttgacct cgcccgtttt
     3001 ggtgaacttg cacgcattgc tcagcaggtt caggagtatc tgccgcagcc gcatggcgtc
     3061 gaccgtcaat gcgccgaggt tctcttgggc gtcgacgaca aggcggttct cgttctgctc
     3121 ggccagctgt cccgcggtgc cgatgacctc attgatcagc ggtgcgagct gcacggtttg
     3181 tggattgagt tcgagctttc cggcctcgat cttcgacaga tcgagcacct ggttgatgag
     3241 gccaagaagg tgagtgccgg cgcggttcac gcgctg
//
LOCUS       DATLJT010000896         4924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_59061,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000896 DATLJT010000000
VERSION     DATLJT010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59061"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..355
                     /locus_tag="VM910_20680"
     CDS             35..355
                     /locus_tag="VM910_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64975.1"
                     /translation="MISTWSDWKRYPRAGRGENIEAPISPGIYEVRFASTGALHSFGA
                     VDNVAQALALLSVGSKSWFGRRDAAEAPDLEYRTCATSTKADAKAAAERMVGRRETYM
                     SGAA"
     gene            461..1957
                     /locus_tag="VM910_20685"
     CDS             461..1957
                     /locus_tag="VM910_20685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVI64976.1"
                     /translation="MTPTAAARAPQPSSPSLHGRLKDASLLRDRCYIDGAWVGTPSRP
                     VTNPVNGSELAKVPVMSTAEATQAVQAAERAFPAWAKLTAKQRSNILRKWFDLIVANR
                     EDLALILTSEQGKPLAEALGEVDIGGAYVEFFAEEARRVYGETIPSQRADARLLAIKQ
                     PIGVCGAITPWNFPCAMITRKVSPALAAGCTVVLKPANETPLTALALVALAEKAGIPK
                     GVFNVLTGNSSAIGKVLCEHPAVRFVGFTGSTEVGKILYQQASVGVKRLGLELGGNAP
                     FVVFDDADIDAAVDGAIVSKYRNMGQTCVCANRIYAQDKIYDEFVQKLSRKVAAMKIG
                     DGTEQGVTQGPLINMDAVDKVEKHIADAVKGGAKVITGGKRHALGGTFFEPTVLADVR
                     PDALVAHEETFGPLAPVFRFKDEAEVIALCNNSPFGLASYFYARDLGRVWRVAEALES
                     GMVGVNTGLITTEVAPFGGVKESGLGREGSRHGMEEYVEIKYVMMAGV"
     gene            complement(2053..2853)
                     /locus_tag="VM910_20690"
     CDS             complement(2053..2853)
                     /locus_tag="VM910_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HVI64977.1"
                     /translation="MSEADAVLIERDGPVTIVTINRPHCRNAVDGATARKLYDAFLAF
                     DADKDASVAVFTGTGGYFCAGADLKAVAAGDPNKKREIGGHDSIAPMGPSRLPLSKPV
                     IAAVEGFAVAGGMELALWADMRVVAEDATFGVFCRRFGVPLIDLGTIRLPRLIGHSQA
                     IDLILTGRPVGGPEALRMGLANRLVPKGEARAHAIALARDIARFPQTCLRADRLSALR
                     QWDLSEEEAIANEMRGGLEVIASGETLSGATRFASGIGRHGAFGDQGH"
     gene            complement(2920..3834)
                     /locus_tag="VM910_20695"
     CDS             complement(2920..3834)
                     /locus_tag="VM910_20695"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVI64978.1"
                     /translation="MTPVSASNLPLPKVGRGKVRDIYAVGDDRLLLLTTDRISAFDVV
                     MAETIPMKGAVLTQISAFWFDKLAGRVPHHMITADADAIIREVPALRAHRPDILGRAL
                     LCRRTTVFPIECVIRGYLSGSAWKEYAAHGTLAGEKLPAGLVESARLEPAIFSPATKA
                     ETGHDENITISRMREVVGREEADTLERMARDIYALGESESRARGIIIADTKFEFGRDS
                     NGRIILIDEVMTPDSSRFWAVDAYRPGRPQPSFDKQPLRDYLDAERRAGRWNGEAPAP
                     SLPDSVVDATSKRYLEAFRRVTGRELKI"
     gene            complement(3846..4763)
                     /locus_tag="VM910_20700"
     CDS             complement(3846..4763)
                     /locus_tag="VM910_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVI64979.1"
                     /translation="MSWLPSNDPILGDPKTCDALDLIIVPRTRDLGDGFAVRRALPHG
                     KRQMVGPFIFFDHFGPVQFVSGKGMDVRPHPHIGLATVTYLFDGSIMHRDSEGNIQEI
                     TPGAMNLMTAGRGIAHSERTPDLQRQSGQKMLGLQSWIALPAGREEIAPSFQHYAAGD
                     LPMISERDFTARVIAGNSFGIASPVEMISPWFYTEVTAAAGASVPLDPDHEERAIYLV
                     DGEVEIANERYEGPLLLIFRPGDRITVRAIQPTRMMFLGGDALEGPRHIWWNFVSSSK
                     ERIEQAKQDWKSGRFAPVPQEHEFIPLPE"
BASE COUNT          885 a         1578 c         1589 g          872 t
ORIGIN      
        1 catgtttgcg gccgaaggcc gcgagaggaa tgcaatgatc tcgacttgga gtgactggaa
       61 acgctatccc cgggcggggc gtggcgaaaa catcgaagca ccgatatcgc cgggcattta
      121 cgaagttcgt ttcgccagca ccggtgcact gcactcgttt ggcgccgtcg acaacgtggc
      181 gcaggcgctc gcgctcttgt ccgtcggttc caaatcctgg ttcggccgcc gcgatgccgc
      241 tgaggcgccc gatctcgagt accgcacctg tgccacctcc accaaggccg atgccaaggc
      301 ggcggcggaa cgcatggtcg gtcgccgcga aacctatatg agcggcgcgg cctgatttcg
      361 gaattactcg aggcggaagc gcgtccggca cccggtgcat cgcggatcga ttgcgcctat
      421 attgggcgca acgaccgcaa atgatccgag gagctacgcc atgaccccga ctgccgccgc
      481 acgcgcaccg caaccttcct cgccgtcgtt gcacgggcga ctgaaagacg cctcgctgct
      541 gcgcgaccgt tgttacatcg atggtgcgtg ggtcggcacg ccgtcgcggc ccgtaaccaa
      601 cccggtcaac ggctccgagc ttgcgaaggt gccggtcatg agcacggcgg aagcaacgca
      661 ggcggtgcag gccgccgaac gcgcgttccc ggcctgggca aagctgaccg ccaagcagcg
      721 ctccaatatc ctgcgcaaat ggttcgatct gatcgtcgcc aatcgcgagg atctcgcgtt
      781 gatcctgact tcggaacagg gcaagccgct cgcggaagca ctcggcgagg tcgacatcgg
      841 cggcgcctat gtcgaatttt ttgccgagga ggcgcgtcgc gtctatggcg agaccattcc
      901 gagccagcgt gccgatgcgc ggctgctcgc catcaagcag ccgatcggcg tttgcggcgc
      961 gatcacgccg tggaatttcc cctgcgcgat gatcacgcgc aaagtgtcgc cggcgctcgc
     1021 tgcgggctgc accgtggttc tgaagcctgc aaacgagacg ccgctcacgg cgctcgcgct
     1081 cgtcgcgctc gccgagaagg caggaatccc aaagggcgtg ttcaacgtcc tgaccggcaa
     1141 ttcctcggcg atcggcaagg tgctttgcga acatcccgcc gtgcgctttg tcggcttcac
     1201 cggctcgacc gaggtcggca agattctcta tcagcaggcg tccgtgggtg tgaagaggct
     1261 cgggcttgaa ctcggcggca acgcgccctt tgtggtgttc gacgacgccg acatcgacgc
     1321 cgcggtcgat ggtgcgatcg tctcgaaata ccgcaacatg ggccagactt gcgtctgcgc
     1381 caaccgcatc tacgcccagg acaagatcta cgacgagttc gtgcaaaagc tctccaggaa
     1441 ggtcgcggcg atgaagatcg gcgacggcac cgagcagggc gtcacccagg ggccgctgat
     1501 caacatggac gcggtggaca aggtcgaaaa acacattgcg gatgcggtga agggcggcgc
     1561 caaggtcatc accggcggca aacgccacgc gctcggcggg accttcttcg agccgactgt
     1621 gcttgccgac gtgaggccgg atgccctggt cgcgcatgag gaaacctttg gaccgctcgc
     1681 gccggtattc cgcttcaagg atgaggcgga ggtcatcgcg ctctgcaaca actcgccgtt
     1741 cggtcttgcc tcctatttct acgcccgcga tctcggccgc gtctggcggg tcgccgaagc
     1801 gctggagtcc ggcatggtcg gcgtcaatac cgggcttatc accaccgagg tcgcgccctt
     1861 cggcggcgtg aaagagagcg ggctcggtcg cgaaggctcg cgtcacggca tggaagaata
     1921 tgtcgagatc aaatacgtga tgatggcggg ggtctgacgc gacacccgcc ggctcctgcg
     1981 cggtcatttc atctccgcag cacggcgatc atgcgattgg cgaaggtggc gttcggccat
     2041 cacggtcctt ccctagtggc cttggtcgcc gaatgcgccg tgacgtccga tgccggaggc
     2101 aaagcgcgtc gcgcccgaca gcgtctcgcc cgaggcgatc acctcgagcc cgccgcgcat
     2161 ttcattggcg atcgcttctt cctccgaaag gtcccactgc ctgagcgccg acaggcgatc
     2221 cgcacgcagg caggtttgcg ggaagcgcgc gatatccctg gcgagtgcga tcgcatgcgc
     2281 gcgcgcctcg cctttcggga ccagccgatt ggccaatccc atgcgcagcg cctccggtcc
     2341 gccgactggc cggccggtga ggatcaggtc gatcgcctgc gagtgcccga tcaggcgcgg
     2401 caggcgaatg gtgccgagat cgatcaacgg cacgccgaaa cgacggcaga aaacgccgaa
     2461 tgtcgcgtcc tccgcgacca cgcgcatgtc ggcccacagc gcaagctcca tgccgcccgc
     2521 aactgcaaag ccttcaaccg ctgcgatcac gggctttgac aggggcagcc ggctcgggcc
     2581 catcggcgcg atcgaatcgt ggccgccgat ctcgcgcttc ttgttcggat cgccggccgc
     2641 caccgccttc agatcggcgc ccgcgcaaaa atagccgccg gtaccggtga acaccgcgac
     2701 cgacgcatcc ttgtcggcgt cgaaggccag gaacgcatcg tagagctttc gcgccgtggc
     2761 gccgtcgacg gcgttgcggc aatgaggtcg attgatagtc acgatggtga ccggtccatc
     2821 gcgttcgatc agcacggcat cggcttcgga catgttgcgc tccctatttt ttccatggtt
     2881 gttccaggcg cgcgaagcgc gaacccggac tgaggcgtgt cagattttta gttcacgtcc
     2941 cgtcacgcgt cgaaacgctt cgagataacg cttgctggtg gcgtcgacca cgctgtccgg
     3001 cagtgaaggc gcgggagctt cgccattcca gcggcccgcg cggcgctcgg catcgagata
     3061 atcgcgcaat ggctgcttgt cgaagctagg ttgcggccgg cccggccgat aggcatcgac
     3121 cgcccagaag cgggaactgt cgggcgtcat cacctcgtcg atcaggatga tgcggccgtt
     3181 cgaatcgcgc ccgaactcga atttggtgtc ggcgatgatg atgccgcggg cgcgactttc
     3241 gctttcgccg agcgcataga tatcgcgtgc catgcgctcg agcgtgtccg cctcctcccg
     3301 gcccacgacc tcgcgcattc gagagatcgt gatgttctcg tcatggccgg tctcggcctt
     3361 ggttgcgggg ctgaagatcg caggctcgag cctggcgctt tcgacaaggc ctgcgggaag
     3421 cttctcgcca gcgagcgtgc cgtgtgctgc atattccttc catgccgaac cggacagata
     3481 gccgcggatc acgcattcga tcggaaacac ggtcgtgcgc cggcacagca aggcgcggcc
     3541 caggatatcg ggccggtgcg ccctcagcgc cggcacctcg cgaatgatgg cgtcggcatc
     3601 ggccgtgatc atgtgatgcg gcaccctgcc ggcgagcttg tcgaaccaga aggccgagat
     3661 ctgcgtgagc accgcgccct tcatcggaat ggtctcggcc atcaccacat cgaaagccga
     3721 gatgcgatcg gtggtgagca acagcaggcg gtcgtcgccg accgcataga tgtcacgcac
     3781 cttgccgcgc ccgacttttg gcaggggcag gttgctcgca gatacggggg tcattgcaaa
     3841 gctcgttact ccggcagcgg aatgaactcg tgctcctgcg gaaccggcgc gaagcggccg
     3901 gacttccagt cctgcttggc ctgctcgatc cgctccttgc tggaggagac gaaattccac
     3961 cagatgtggc gcggaccttc cagcgcgtcg ccgcccagaa acatcatgcg cgtcggctgg
     4021 atcgccctca ccgtgatgcg gtcgccgggc cggaagatga gaaggagcgg cccttcgtag
     4081 cgctcgttcg cgatctcgac ctcgccgtca accagataga tcgcgcgctc ttcgtgatcg
     4141 gggtcgagcg gtacgctcgc ccctgccgca gcggtcactt cggtataaaa ccagggcgag
     4201 atcatctcga ccggcgaggc gatgccgaac gaattgcctg cgatgacgcg cgccgtgaaa
     4261 tcgcgctccg agatcatcgg aagatcgccg gccgcgtaat gctggaaaga cggcgcgatc
     4321 tcttcccgtc cggccggcag cgcgatccag ctctgcaagc ccagcatctt ctgcccgctc
     4381 tggcgctgca gatctggcgt tcgctcggaa tgggcgatac cgcggcccgc cgtcatcagg
     4441 ttcatcgcgc cgggcgtgat ctcctggata ttgccctcgc tgtcacggtg catgatgctg
     4501 ccgtcgaaca aataagtgac ggtggccagc ccgatatggg gatgcggccg cacgtccatg
     4561 cccttgccgg acacgaattg caccgggcca aaatgatcga agaagatgaa gggtccgacc
     4621 atctgccgct tgccatgcgg cagcgcgcgg cggacggcaa atccgtcgcc gaggtcgcgg
     4681 gtacgcggta cgatgatcag atcgagtgcg tcgcaggttt tgggatcgcc gaggatgggg
     4741 tcattggaag gcagccagct catgaaggcc tccattgttt tggcgcgacc ttaacaggaa
     4801 ttgtatgatc cgtcgtccgg cattcgcgag gacgacgcga caggttcgag gggatgacaa
     4861 gcatgatcgc tccacgcagg cccgacgtgc tggcccaact gcacggaccg atgatcgaaa
     4921 ccaa
//
LOCUS       DATLJT010000897         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1667867,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000897 DATLJT010000000
VERSION     DATLJT010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667867"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VM910_20705"
     CDS             complement(<1..1496)
                     /locus_tag="VM910_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HVI64980.1"
                     /translation="MPEQKPISRVNDFVVRFANVNGSGSASANELFARAILRHGVPVS
                     PRNIFPSNIQGLPTWYEVRVTEAGHLGARGGVDMMVAMNPQTWDKDVGSIESGGYLFY
                     DSTKPMPSSKFRSDITVIGVPLTAITNSTYTDPRQRQLFKNIIYLGALCALLDMDPKL
                     IEQLIGEQYKGKEKLLSSNVHALHLGRDWALQNLKCPIGLRVKKANKVGDRIFIEGNS
                     AAALGAVYGGATVCAWYPITPSSSLADAFASHCKRLRHDPATGKAKYAIVQGEDELAS
                     IGIVIGASWNGARAFTATSGPGISLMTEFIGLSYFAEVPAVIMNVQRAGPSTGMPTRT
                     QQCDIIACAYASHGDTKHVLLFPEDPAEAFEFAAQAFDLAERLQTTIFLMLDLDIGMN
                     HRLCRPLRWDDARQYDRGKIMTAEMLEEGRDFGRYLDIDGDGIPYRTYPGTHPTKGSY
                     FTRGTSRDRYARYSEEGPVYADNMQRLVRKFETAQDMVPRPLQANAAKP"
     gene            complement(1466..>2722)
                     /locus_tag="VM910_20710"
     CDS             complement(1466..>2722)
                     /locus_tag="VM910_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI64981.1"
                     /translation="SMKKLLADGYDAIFVGSGAPRGRELDVPGRKEAAAHIHIGIDWL
                     SSVSFGHTDKIGKRVIVLGGGNTAMDCCRTARRLGGEDVKVIVRSGFDEMKASPWEKE
                     DAIHEDIPILNYMVPIAFVHNNGRLTGVTFQRVKAEYDARGRRNLVPTGEADETINCD
                     DVLVAVGQENAFPWIERDCGIEFDKWDMPRVDPKTMGSTHPKVFFGGDAAFGPKNIIW
                     AVAHGHDAALSIHKLCSGEDVNERPLPEVQITSQKMGIHEWSYDNDISIDKRFKVPHR
                     DKVIALKDIRAEVELGYDVKLALGEAHRCLNCDVQTVFTTSLCIECDACVDICPMDCI
                     TFTENGDEADLRQRLKAPSVHPDQDLYVSSDLKTGRVMVKDEDVCLHCGLCAERCPTG
                     AWDMQKYLIDMTYAGTSCPSKSRSAA"
BASE COUNT          469 a          818 c          894 g          541 t
ORIGIN      
        1 ggttttgccg cattggcctg caagggccgc ggcaccatgt cctgggcggt ttcgaacttg
       61 cgcaccagtc gctgcatgtt gtcggcataa accgggcctt cctcggaata acgcgcgtag
      121 cggtcgcgcg aggtgccgcg ggtgaaatag gagcccttgg tcgggtgcgt gccgggatag
      181 gttcgatacg ggatgccgtc gccgtcgatg tcgagataac ggccaaaatc gcgaccttct
      241 tccagcattt ccgccgtcat gatcttgccg cggtcatatt gccgcgcatc gtcccagcgc
      301 agcggacggc acagccggtg gttcatgccg atatcgagat cgagcatgag aaagatcgtc
      361 gtctgcagcc gctcggcgag atcgaaagcc tgggccgcga attcgaaggc ttccgccggg
      421 tcttccggaa acagcagcac atgcttggtg tcgccgtgcg aggcataggc gcaggcgatg
      481 atgtcgcact gctgcgtgcg cgtcggcatg ccggtcgatg ggccggcgcg ctgcacgttc
      541 atgatgacgg cgggaacttc cgcgaaataa gacaggccga tgaactcggt catcagcgag
      601 atgccggggc ccgaggtcgc agtgaacgcg cgggcgccgt tccaggaggc gccgatgacg
      661 atgccgatcg aggcgagttc gtcctcgccc tgtacgatcg catatttcgc cttgccggtg
      721 gccggatcgt gccgcaggcg tttgcagtgg ctcgcgaagg cgtcggcgag cgacgaggag
      781 ggcgtgattg gataccaggc gcagacggtg gcgccgccat agacggcgcc gagcgcggcc
      841 gcgctgttgc cttcgatgaa aatgcggtcg ccgaccttgt tggccttctt cacgcgcagc
      901 ccgatcgggc atttcaggtt ctgcagcgcc cagtcgcggc cgagatgcag cgcgtggacg
      961 ttggaagaca aaagcttctc cttgcccttg tactgctcgc cgatcaattg ctcgatcagc
     1021 ttcgggtcca tgtcgagcag cgcgcagagg gcgccaagat agatgatgtt cttgaagagc
     1081 tgacgctggc gcggatcggt ataggtcgag ttggtgatcg cggtcagcgg cacgccgatc
     1141 acggtgatgt cggatctgaa tttcgacgac ggcatcggct tcgtcgaatc gtagaacaga
     1201 taaccgccgg actcgatcga gccgacgtcc ttgtcccagg tctgcgggtt catcgcgacc
     1261 atcatgtcga cgccgccgcg cgcgccaaga tggccggctt ccgtcacacg cacctcatac
     1321 caggtcggca ggccctggat gttggagggg aagatgttgc gcggcgacac cggcacgccg
     1381 tggcgcagga tcgcgcgggc aaacagctcg ttggcgctgg ccgagcccga gccgttgacg
     1441 ttggcgaagc gaacgacgaa gtcgtttacg cggctgatcg gcttttgctc gggcatgatg
     1501 ttcctgcgta agtcatatcg atgagatatt tctgcatgtc ccaggcgccg gtggggcagc
     1561 gctcggcgca cagcccgcaa tgcaggcaga cgtcttcgtc cttcaccatg acgcgtccgg
     1621 tcttcaggtc gctcgaaacg tagaggtcct ggtcgggatg caccgatggc gccttcaggc
     1681 gctgccgcaa gtccgcttcg tcgccgttct ccgtgaaggt gatgcaatcc atcgggcaaa
     1741 tgtcgacgca ggcgtcgcat tcgatgcaaa gcgaagtcgt gaacacggtc tgcacgtcgc
     1801 agttcaggca gcgatgcgcc tcgcccagcg ccagcttgac gtcgtagccg agctcgacct
     1861 cggcgcggat atccttcagt gcgatgacct tgtcgcgatg cggcaccttg aagcgcttgt
     1921 cgatggagat gtcgttatcg tagctccatt cgtgaatgcc catcttttgc gaggtgatct
     1981 gcacctcggg cagcggacgt tcgttgacgt cttcgcctga gcagagcttg tggatcgaga
     2041 gtgcggcatc gtgaccgtgc gccaccgccc agatgatgtt cttcgggccg aaggcggcgt
     2101 cgccgccaaa aaacactttc ggatgggtcg agcccatggt cttcggatcg accctgggca
     2161 tgtcccattt gtcgaattcg atgccgcaat cgcgttcgat ccaggggaac gcattctcct
     2221 gtccgaccgc gaccagcacg tcgtcgcaat tgatcgtctc gtcagcttcg ccggtcggca
     2281 ccaggttgcg ccggcccctg gcatcgtatt cggccttcac cctctggaag gtgacgccgg
     2341 tgagccggcc gttgttgtgc acgaaggcga tcggcaccat gtagttgagg atcggaatgt
     2401 cctcgtggat ggcgtcttcc ttttcccagg gcgaggcctt catctcgtcg aaaccggagc
     2461 gcacgatcac cttgacgtcc tcgccgccga gacggcgcgc ggtgcggcaa cagtccattg
     2521 cggtattgcc gccgccgagc acgatcacgc gcttgccgat cttgtcggta tggccgaacg
     2581 acacgctcga gagccagtcg atgccgatgt gaatatgggc ggcggcctcc ttgcggccgg
     2641 gaacgtcgag ctcgcggccg cgcggcgccc cggagccgac gaagatggca tcgtaaccgt
     2701 ccgcgagcag cttcttcatg ct
//
LOCUS       DATLJT010000898         5504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3275694,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000898 DATLJT010000000
VERSION     DATLJT010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3275694"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1580
                     /locus_tag="VM910_20715"
     CDS             303..1580
                     /locus_tag="VM910_20715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVI64982.1"
                     /translation="MGHLLSPPASPSDPLMWADFQALCDLGGRVAGSASEAAALAFAR
                     ERLAAVPGARVHDDPVDYPGWRCQTAQLVSAMTGVSFPCTPLLGTASAAGVVAEALDI
                     GLGRVEDFERHADRIRGRIVMVRHEYPFAGAHVHRRVKLSLAQRMGAVGFLIAHPEQG
                     VGAVSGSSGRNGGAGIPALGIGVEAADAFRRSPDGPLPTARMVIDGDDHPARTCMLIA
                     DLPGRGPDWVVVSAHIDGHPHGESAMDNATGVAVALALARLLAPHVCECPRGLRICLF
                     SAEEWGLVGSRIWLERMDEAARRSMVINLNLDSVGGASALTALTSGFERLDAWVKDTA
                     VRAGLPIATHVPLMANSDHANFAAHGIPAVRLLAGFNAPDSNLRLVLTAADTRDKVQP
                     EELDQALRIATTLTWQALSAADATLSSLAAGRG"
     gene            complement(1609..1827)
                     /locus_tag="VM910_20720"
     CDS             complement(1609..1827)
                     /locus_tag="VM910_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64983.1"
                     /translation="MSVNTDETRRRAETLFKKEQQLREAQQAMAEYQAQLHATREKTA
                     RLRALRLARDAANNNKKKMRLASQKGAA"
     gene            2122..2550
                     /locus_tag="VM910_20725"
     CDS             2122..2550
                     /locus_tag="VM910_20725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI64984.1"
                     /translation="MPVLLRRATEADAKHVESLLAELGYPSKEADVRHRLRNSLRSDT
                     SCFLLAQSASEVVGLVCAELVPYFPHGSTICRVTGLVVSAQHRGQGVGEKLLAGAADF
                     ARENHCSGIEVTSAEHRLDAHRFYQRLGFSRTSFRFFQAL"
     gene            complement(2637..3542)
                     /locus_tag="VM910_20730"
     CDS             complement(2637..3542)
                     /locus_tag="VM910_20730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64985.1"
                     /translation="MRRVGVLMSTTENDVEGQARIGAFLQGLRQAGWAVPGNLRIDTR
                     WAAGDAERIRKYAAELFALAPDVILASGGSVVGPLLKLSHTVPIVFTLTPDPVGAGFV
                     QSLAHPGGNATGFTSIEYGMSGKWMELMREIAPGVTRVAVLRDPTLPAGIAQFAILQT
                     AATSLGTELSSIGGRDAAEIEGAVTEFARTSNGGLIVLGSAVTVLHRDLIIALAARHA
                     LPAVYPGTYFVTSGGLISYSADSTDPHRRAAGYVDRILKGDKPANLPVQNPVKYELAV
                     NLKTAKALGFKIPDRLLAIADEVIE"
     gene            complement(4164..4397)
                     /locus_tag="VM910_20735"
     CDS             complement(4164..4397)
                     /locus_tag="VM910_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64986.1"
                     /translation="MGTSDDSFRAEGLGLFGFLTDSDDDNVPVKAFGSVGYALNVGVR
                     AHDDGRTVVIGGEAEVDERVASPAGVVDDPNRS"
     gene            4558..>5504
                     /locus_tag="VM910_20740"
     CDS             4558..>5504
                     /locus_tag="VM910_20740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI64987.1"
                     /translation="MKRVLCALIFCLVCVLAVDVNAQSWPNRPLKLIVPTGPGAATDV
                     MARMLADGVSRNLDQSMVVENMPGASGIVAHQTVARAAPDGYTFLFTNTSGMAINLIS
                     FKQLPYDPTKDFTAVAMVCNQGPQMISVNADLPVRTLSDLIAYAKANRGKLSIAFDTT
                     AGAAAFAAKLFNRRADLGLVEVPYRSAAQMTQDVASGVNQVMISSIAAARSVVDADKV
                     RPIAITSKTRFRGLPDLPAVSETLPGIAIDGFFGIVAPAGTPADIVARLNREIAEYLK
                     GPEIQQRLISFGLATEGAGTPESTAQFIRKEQDRWRALA"
BASE COUNT          977 a         1733 c         1729 g         1065 t
ORIGIN      
        1 tttgaacaat cgctgatcag gtgattggtc gaaccaacta ccaaatgggg atggggcccg
       61 ataagccgca attttgctgc aggtgcgaat gtcgccttgt ggcacaaagc ggacatattg
      121 agccaagctc gcgatgtccg ctttgggggg taaagcggac atggctctaa gtccttgaat
      181 gtctgctttt ggcactaagc ggacatgtca cgatgtctgc cttatgtccg ctaagcaccc
      241 caaagcggac atgatcagcc cgccctcgac tctcgcgatt taatgagtac acgtctagat
      301 cgatggggca tctgctctcg ccgccggcaa gtccgagcga cccgctgatg tgggcggact
      361 ttcaggcgct gtgcgatctc ggcggccgcg ttgccggctc ggcgagcgaa gccgcggcgc
      421 tggccttcgc ccgagaacgg ctcgcggccg tgccgggcgc aagggttcac gacgatcccg
      481 tggactatcc gggttggcgg tgccagacgg cgcaactggt gagcgcgatg acgggcgttt
      541 cgttcccatg cacgccgctt ctgggcacgg catcggcagc gggcgtcgtg gcggaagccc
      601 tcgacatcgg cttgggccgc gtggaggatt tcgaacggca cgcggaccgc atccgcggcc
      661 gcatcgtgat ggtgcgccac gaatatccgt tcgcgggcgc ccacgtgcac cgccgcgtca
      721 agctctcgtt ggcgcagcgg atgggcgcgg tcgggttcct gatcgcgcat ccggaacagg
      781 gcgtcggcgc cgtatcgggg tcgagcggac gcaacggcgg cgccggcatc ccggcgctcg
      841 gcatcggcgt cgaagcggcg gacgcgttcc gccgttcgcc ggacggcccg cttccgactg
      901 cccgcatggt catcgacggc gatgatcatc ccgcgcgcac gtgcatgctg attgccgacc
      961 tgccgggccg aggcccggat tgggtcgtgg tcagcgcgca tatcgacggc catccgcatg
     1021 gcgaaagcgc gatggataac gccaccggcg tggcggtcgc gctcgcgctt gcacgcttgc
     1081 tcgcgccgca cgtatgcgaa tgtccgcgcg gcttgcgcat atgcctgttc agcgcggagg
     1141 aatggggcct cgtcggatcg cgcatctggc tcgaacgaat ggacgaggcc gcgcggcggt
     1201 cgatggtcat caacctcaat ctcgacagcg ttggcggcgc atccgccttg acggcgctga
     1261 cctccggctt cgaacgtctc gatgcctggg tcaaggacac cgccgtgcgc gccggtctgc
     1321 cgatcgcgac gcacgtgccg ctcatggcca attccgatca cgccaatttt gcggcgcacg
     1381 gcattcccgc cgttcggctg ctcgccgggt tcaacgcgcc cgacagcaat cttcgtctcg
     1441 tgctgaccgc cgccgatacc cgcgacaagg tgcagccgga ggaactcgat caggcgctga
     1501 ggatcgcgac gacgctgacg tggcaggcgt tgagcgcggc cgatgcgacg ctgtcgagcc
     1561 tagcggcggg gcgcggttag atcgctggcc ccgagcttgg cccgcaactt acgctgcgcc
     1621 tttctggctc gccagccgca tcttcttctt gttgttgttg gcggcgtcgc gcgccaaccg
     1681 cagcgcccgc aagcgcgcag tcttttcgcg cgtcgcatgc agttgcgcct ggtactcggc
     1741 cattgcttgc tgggcctcgc gcaattgctg ttccttcttg aacagcgttt cggcgcggcg
     1801 gcgcgtctcg tcggtattca cgctcactat tggaactcca tgtaaagccc tagaggatcg
     1861 tggcctttcg atttgcccac ctggtcttgc ggccgatcaa aacgacatcg tggccagcaa
     1921 cttctgtctg gcgggcaagc taggacatgc gtcgtctgag tagataagct ctatatggtt
     1981 acagttgaag aaataacaag ggtctcggat cgattgcgat gtccgcgtca cctccaattg
     2041 ctaccgattg atgcaaagcc gcgagctgac gcagtgtgcc gattgcggat tttctggggc
     2101 ttcaaacgct gaagggcgcg catgccagtc ttgctcagac gtgcgacgga agctgacgcg
     2161 aagcatgtcg agtcgctatt ggccgaactc ggctatccgt cgaaggaggc ggacgtgcgc
     2221 catcgcctgc gtaattcgct acgcagcgac acgagctgtt ttctccttgc acaatcggca
     2281 agcgaggtcg tcggcttggt ttgcgccgaa ctcgtcccat attttccaca cggctcgacg
     2341 atttgcaggg tcactggtct cgtcgtctcg gcgcagcatc gtggccaggg tgtcggtgaa
     2401 aagcttcttg ccggggcggc cgatttcgca cgcgaaaatc attgctcagg cattgaggtc
     2461 accagtgcgg agcatcgtct cgacgcgcac cggttctacc agcggctcgg attctcccga
     2521 acctcgttca gattcttcca ggccctataa acagcgtggc gacatcgtcg ccgcggggct
     2581 caccgctcga tcgcgccgca accccgcaac ctgcgccgtg cgcggttgca acggatctat
     2641 tcgatcacct cgtcggcgat cgcgagcagc cggtcgggaa ttttgaagcc gagcgccttt
     2701 gcggtcttga ggttgaccgc cagctcgtac ttgaccgggt tctgcaccgg aaggttggcc
     2761 ggcttgtcgc ccttgaggat gcgatcgacg taaccggccg cgcgccggtg cgggtcggtc
     2821 gagtcggcgg agtaggagat gagcccgccg ctggtgacga agtaggtccc ggggtagacc
     2881 gccggcagcg cgtgccgggc cgcgagcgcg atgatcagat cgcgatgaag caccgtgacc
     2941 gcgctcccga gcacgatcaa accgccgttc gaggtgcgcg cgaattccgt gacggcgccc
     3001 tcgatctcgg ccgcgtctcg cccgccgatc gagctcaact ccgtcccgag cgacgtcgcc
     3061 gcggtctgga ggatggcgaa ctgcgctatt cccgcgggca gggtgggatc gcgcagcacc
     3121 gccacgcgcg tcacgccggg cgcgatctcc cgcatcagtt ccatccattt cccgctcatg
     3181 ccgtattcga tcgaagtgaa accggtggca ttgccgcccg ggtgcgccag gctttggacg
     3241 aaaccggccc cgaccggatc gggggtcagc gtgaacacga tcggcacggt gtggctcagc
     3301 ttcagcaacg gcccgacgac cgagccgccg ctggccagga tgacgtccgg cgcgagcgca
     3361 aagagctccg ccgcgtattt gcgaatgcgc tcggcatcgc ccgcagccca gcgcgtgtcg
     3421 atgcgcaggt tgccggggac ggcccagccc gcctgccgca gcccctgcag gaatgcgccg
     3481 atgcgggcct gtccttccac atcgttctca gtcgtggaca tgagtacgcc gacgcgccgc
     3541 atccgctcgc tctgctgcgc gcgcgccgcg aacggatacg ccgctgccgc gcccgcgagc
     3601 agcgatatga actggcgccg tctcatgcgc tcgcctcgag ccgagggtca cacctcggtt
     3661 gaggctttct atcacatgtc ggaagcgttg tgggtcacgg caaatttgcc cggcaacgtc
     3721 cgggccgggt caaaaaagcg gccctgtgcc gtcacttagg ccaaggtcgc ttgatccccc
     3781 gagcatggaa ctgatcgatc gacggaacgg ctgcgcgcgc ggtgcgttgc ctcaggcgcg
     3841 ggtccagcaa gcttagctct ggcgggggaa caacagcctg aggagcccgc gatgaggaat
     3901 attttaggca tcgttgtgtt tgctgtagcg ctgatgggcg gcgcgagcgt tgcgctggcc
     3961 caatccagtc gggacctgac cgagcccacg ggcacggagg cgaaccgcat aaagacccaa
     4021 acagggggct attacggccg tgggcatcgc gcgtatgcgt actcgtcggg cctgcaccga
     4081 cgatggcatt ggtctcaccg gcgttggtga tttgtcgcaa cagcccacaa agacgatagt
     4141 tgcaatcaac tcatctgtgc cgcttatgac cggttggggt catccacgac cccggccggc
     4201 gaagccacgc gctcatccac ctccgcttca cctccgataa cgaccgttcg gccatcatca
     4261 tgcgctcgca cgcccacatt caaggcatac ccgacggaac cgaaggcctt gaccggaacg
     4321 ttgtcgtcgt cagagtcggt cagaaagccg aatagcccga gcccctcggc cctgaagctg
     4381 tcgtcgctgg tgcccattgt cgcctcctgt ggattgttgc gatggcgcgc aggatattgc
     4441 ccaccaataa aagcgtccca atcaggacgt ccctccattc gcttgcaact gtcgtaggtc
     4501 aacatacact caagcagttg tgttggaaaa agacatgatc agcgttcggg agcacccatg
     4561 aaaagggttc tatgcgcgct gattttttgc ttggtctgtg tccttgccgt cgatgtcaat
     4621 gcgcagagtt ggccaaatcg cccacttaaa ctgattgttc ctaccggtcc cggcgccgcc
     4681 accgacgtga tggcgcgaat gttggccgat ggtgtttccc gaaatctcga ccagtccatg
     4741 gtggttgaaa acatgcctgg cgcttcgggc atcgtggcgc atcagacagt cgctcgcgcg
     4801 gcacccgatg gatatacgtt tctgttcacc aacacctctg gtatggcgat caacctgatc
     4861 tcattcaagc aactgcccta cgatccaacc aaagacttca cggctgtcgc aatggtctgc
     4921 aatcaaggac cgcagatgat atccgtgaat gctgatctgc cggtgaggac gctctcggac
     4981 ctgattgctt acgcgaaggc taatcgtggc aagttgtcga tagcctttga tacgacggca
     5041 ggcgcggcgg cattcgccgc caagctgttc aatcggcgcg ccgatctagg cttggtcgaa
     5101 gtaccttatc gatcagcagc ccaaatgacc caggatgtag cgagcggagt taatcaggtc
     5161 atgatcagtt ccattgcagc cgcgcgatcc gtggtcgatg cggacaaagt gcgccccatc
     5221 gccataacgt ccaagacgcg atttcgtggc ttgcccgacc tgcccgcggt gagcgaaaca
     5281 ctgcctggca tagcgatcga tggcttcttc gggatcgttg cgcccgccgg cacgccggct
     5341 gatatcgttg ctcgcctaaa tagagaaatc gccgagtacc tcaagggccc cgaaatccaa
     5401 cagcggctga tatcattcgg cctggcgact gaaggagccg gcacgccgga gagcaccgct
     5461 caattcattc gcaaggagca ggatcgctgg cgtgcactgg caca
//
LOCUS       DATLJT010000899         3823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3735431,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000899 DATLJT010000000
VERSION     DATLJT010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3735431"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VM910_20745"
     CDS             <1..1055
                     /locus_tag="VM910_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HVI64988.1"
                     /translation="LTVSRPQFSAPGTVMVRPLLLKPQWMNGLSERLLVSHYENNYGG
                     ALRRLNAIRARLAALDWARSPVFEINSLKREELIVAGSVILHEIYFESLGGHGDNPPT
                     GFEEPPAGLARALERDFGSVMAWRAEFTAMAKALAGGSGWVILAWSERLGCLVNQWAA
                     DHAHGLPGATPILALDMYEHAYHLDFGAKAAAYVDQIMANLNWERIGARYRLAIGDEA
                     AEAKLFLPFGAPVQEEARISAEELKAALEDNNDHRPVLLDLCLPKDLPRRTDMLAGAS
                     MHAPAALPTWVEGLQKDRPIAVYCICGFQVSGTAVTELRGRGYDARALVGGITAWHAI
                     GGATVPLDTSTYEKAP"
     gene            1106..2146
                     /locus_tag="VM910_20750"
     CDS             1106..2146
                     /locus_tag="VM910_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HVI64989.1"
                     /translation="MSTESTARLGRVAILWRGDQAARRSATADSSRFKAVFAALADVG
                     VDAEPVVYEDDVFGAVRAQLATVDGVLVWVNPIHEGRNRAKLDALLREIAARGVWVSA
                     HPDVILKMGSKEVLHRTRTMSWGSDTALYRTAEAMRAELPARLAAGPRVIKRNRGNGG
                     QGVWKVETLKSPRSRPMVCVLDATKDASEQLALDAFLERCVEYFDDGCVIDQPFQTRI
                     SEGVVRCYMAGDSCAGFGHQKVKALVDAPAARADAGPRRYTSNAEPRFQRLRRLMEDE
                     WTPQLTSLLTIPQHDLPMIWDADFMLGPPGPDGIDSYVLGEINVSSVFPIPMEASAEI
                     ARRVADRLRSKP"
     gene            complement(2546..3121)
                     /locus_tag="VM910_20755"
     CDS             complement(2546..3121)
                     /locus_tag="VM910_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HVI64990.1"
                     /translation="MKRISAILLAGVAAFAANATFGELVPTAAGETTAMVGGAPMYPS
                     KNIVQNAVNSKDHTTLVAAVKAAGLVQTLEGPGPFTVFAPTNDAFAKLPDGTVATLLK
                     PENKQTLVNVLTYHVVPSRLTTKDLHERIKAGGGRAELKTVQGGTLTIEEKNGRLWVI
                     DAKGDTAEITISNVMQSNGVIQVINTVMLPS"
     gene            complement(3213..>3823)
                     /locus_tag="VM910_20760"
     CDS             complement(3213..>3823)
                     /locus_tag="VM910_20760"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVI64991.1"
                     /translation="REAATRRVARERAFAALVAKWEERLAPWAAEIPELAPPPQVWER
                     IAAALPRATGQRAGFWQSLVFWRSFGVVSALAAACLAVLLYLSAGSHQAALVASIEGQ
                     GQRIFVASVDVKHATVTVMPVAYRPDPTRVPELWLIPSGGKPLPLGVLTADRTAQIAI
                     PPALAAQARRDGILAVSLEPPGGSPTGQPTGPVIGSGRLTNL"
BASE COUNT          694 a         1164 c         1224 g          741 t
ORIGIN      
        1 cgctcaccgt atcccgccca caattctccg cgcccggaac cgtgatggtt cggccgcttc
       61 tgctcaagcc gcaatggatg aatgggctct cagagcgact gctcgtgagc cattacgaga
      121 ataactatgg cggcgcgctg cgaagactga acgccatcag agcccgtctt gcggccctcg
      181 actgggcgcg gtcgcctgtg ttcgagatca atagcctcaa acgcgaagag ctgattgtcg
      241 cgggttctgt catcctgcac gaaatctact tcgagtcgct cggtgggcac ggcgacaatc
      301 cacccaccgg atttgaagag ccgcccgcgg gactcgcccg ggcgctcgag cgtgatttcg
      361 gcagcgtcat ggcttggcgt gcggaattta cggccatggc aaaggcacta gcaggcggtt
      421 cgggctgggt gatcctcgct tggtccgagc gcctgggctg cttggtcaac cagtgggcgg
      481 ccgaccacgc gcacggcctg ccaggggcca ctccgatcct cgcgctcgac atgtatgagc
      541 atgcctatca tctcgatttt ggcgcgaagg ccgctgccta cgtcgatcag ataatggcaa
      601 acctgaattg ggaacggatc ggcgcgcgtt atcgcttggc aatcggcgac gaggcggcag
      661 aagccaagct cttccttccg ttcggcgcgc cggtgcaaga agaggcgcgg atttcggccg
      721 aggagcttaa ggcggcgctc gaggataaca acgatcaccg accggtactt ctagatctct
      781 gtctgcccaa ggacctaccg cgacgcaccg atatgttagc gggtgcctcc atgcacgctc
      841 ctgcggcgct gccgacttgg gtcgaagggc tgcaaaaaga ccgaccaatc gcggtctatt
      901 gcatttgcgg atttcaggtc agcggcaccg cggtgaccga gctgcgaggg cggggttatg
      961 acgcccgggc tctcgtcggc gggattaccg cctggcatgc gattggcggc gcgaccgttc
     1021 cgctggatac ctctacgtac gagaaagcgc cataaaaaat tgcgcgaagg caaactgcgc
     1081 gggttaacac agctgggtga ttagcttgag cacggaatcc acagctcggc ttggccgcgt
     1141 cgctattcta tggcgcggcg accaagctgc tcgccgtagt gccacggcag atagcagccg
     1201 gttcaaggct gtcttcgcgg cgctcgctga cgtcggcgtc gatgccgaac cggtggtcta
     1261 tgaggatgat gttttcggcg ccgtgcgcgc acaactcgcc acagtcgatg gtgtgctcgt
     1321 ctgggttaat ccaattcacg aggggcgtaa tcgagccaaa ctcgacgcgc tgctgcgcga
     1381 gattgcggcg cgcggcgtct gggtgagcgc ccatcccgat gtgatcctca agatgggcag
     1441 caaggaggtg cttcatcgca cccgcacgat gagttggggc agcgacacgg ccctctatcg
     1501 aacagccgag gccatgcgcg ctgaattgcc ggcgcggctc gctgctggcc cacgcgtgat
     1561 caagcgcaat cgcggcaacg gcggccaggg cgtctggaag gtcgaaacgt tgaaaagtcc
     1621 acgcagccga ccaatggtat gcgtgctgga tgccaccaag gacgcatcgg agcaactggc
     1681 gctggacgcg tttctcgagc ggtgcgtcga gtatttcgac gacggctgcg tgatcgatca
     1741 gccgttccag acacgcataa gcgaaggtgt ggtgcgctgt tacatggcag gcgatagctg
     1801 cgccggtttc ggtcaccaga aggttaaggc tctggtcgac gcaccggccg cgcgcgccga
     1861 tgctggaccg cggcgctaca cgtccaatgc tgagccgcgc ttccaacggc tgcgccggct
     1921 gatggaggac gagtggacgc cgcagctaac ctccttactg accatcccgc aacacgacct
     1981 tccgatgatc tgggatgcgg atttcatgct cggcccgcct ggccctgacg gaatcgacag
     2041 ctacgtgctt ggcgagatca acgtcagttc ggtgttcccg atccccatgg aggcgtccgc
     2101 agagatcgcg cgtcgggttg ccgatcggct gcggtcgaag ccctaagggg atcagtgttc
     2161 gggagttcat tgcgtgcctt ggcaacttgg aggtgtgcca gccggacttt ccagaaacac
     2221 tttcctcctc gccggcggtc atattccttc ccaacattgc aacgtatggt tccgtgccac
     2281 ccattgtagc ttgcactgcg ggtactcggc cgctcgatgt ccgctttggg tcaaaagcag
     2341 acattgcggc acctccaacc aatgtccgct ttacccccga aagcggacat cgctgatcac
     2401 gactgccatg tccgctttgg gcctattaac ggacatcggt ggctgacatt cgatagcctc
     2461 ggcggcggtc gaacgtcctc tcccggccgg ccggaagagg acgttccctg catcggtcta
     2521 cgcgttgcac tcgatgacga gcaaatcagc taggcagcat cacagtattg atgacctgga
     2581 taacaccgtt tgactgcatc acgttgctga tggtgatttc ggcggtgtcg ccctttgcat
     2641 cgatgaccca gagcctgccg ttcttttcct cgattgtcag cgtaccgcct tgcaccgttt
     2701 ttagctccgc tctgccgccg ccggctttga tgcgctcatg caagtccttg gtggtgaggc
     2761 ggcttggcac gacgtgatag gtcaacacgt tgacaagtgt ttgtttgttc tcgggcttga
     2821 gcagtgtcgc caccgtgccg tccggcagct tggcgaaagc gtcgttggtc ggcgcgaaca
     2881 ccgtgaacgg tcccggacct tccagagtct gcacgagccc cgccgccttc accgcggcaa
     2941 ccaacgtagt gtgatctttg gaatttacgg cgttctgtac gatgttcttg gacgggtaca
     3001 tcggcgcgcc gccgaccatc gcggtcgttt cgcctgcggc cgtcgggact agctcgccga
     3061 aagtggcgtt agcggcgaag gcggcaacgc ccgccaggag gattgctgaa atgcgtttca
     3121 tggttcgctc cttctcatga ctcttcgttc gtccccatga tgaggacgtt gacaggtacg
     3181 gagcgacgag agttacggat gcgtggcggc gctcacaggt tcgtgagcct gccgctgccg
     3241 ataacggggc ctgtcggctg cccagtgggc gaaccaccgg ggggctcgag cgagacggca
     3301 aggatgccgt cgcgcctggc ctgagcggca agagccgggg gaatagcgat ctgggcggtg
     3361 cggtcagcgg tcaaaacacc gagcggcagc ggtttgccgc cggagggaat cagccacagc
     3421 tcagggacgc gtgtcgggtc gggccggtag gcaaccggca tcaccgtaac tgtggcatgc
     3481 ttgacgtcga cggaggcaac aaaaatgcgc tggccctggc cttcgatgga agcgacaagc
     3541 gccgcctggt gtgagcccgc gctgagatat agcagaacgg ctaggcaggc agccgcgagc
     3601 gcgctcacca ccccgaagct gcgccaaaat acgaggctct gccaaaagcc ggcgcgttgt
     3661 cctgttgcgc gggggagcgc cgccgcgatc cgttcccaaa cctgcggcgg tggagcgagc
     3721 tctgggatct ccgccgccca gggcgcgagc cgctcttccc acttcgcaac caatgccgca
     3781 aacgcgcgct cgcgcgcgac gcgccgtgtg gcagcctcgc gtt
//
LOCUS       DATLJT010000900         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_519180,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000900 DATLJT010000000
VERSION     DATLJT010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519180"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1437
                     /locus_tag="VM910_20765"
     CDS             466..1437
                     /locus_tag="VM910_20765"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64992.1"
                     /translation="MRRRQFITLLGGAAALPLAARAQQPAKMLRVGTVAGTPKSSPQW
                     VAFERRMGELGYQEGKNFSFDFLQAASADEDEVLYRKLAVHMPDVILAIGPEVGLKSA
                     LAVTRTLPIVMIAIDYDPFARGYVTNLARPSGNVTGVVFQQIELAAKRIQLIKDAFRD
                     RPAATMFWDRLSADQWQAAQSAAATLGLQLSGIELRELPYDYDAALDQAPLDHRDILI
                     VPTSPFFFRDRARLADFALRHRILSMFVFREWVDAGGLLCYGPSITALFGRVAEFVDR
                     IARGAKPADLPIEQPTKFEMVVNLKTARAIGIDLPTAILLRADEVIE"
     gene            1686..2669
                     /locus_tag="VM910_20770"
     CDS             1686..2669
                     /locus_tag="VM910_20770"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI64993.1"
                     /translation="MSSIKRREFITLLGGAAAWPLAARAQQSAPPVIGFLSSRSADDS
                     ARVVAAFRQGLAETGYVEDRNVVIEFRWAEGQLDRLPALASELVHRPVAVIAALGESG
                     YAAKAATTTIPIVLGSGGDPVELGLVTSLNRPGGNVTGATFLTAQLGAKRLGLLRDLV
                     PGADVVALLINPNTAVGRVQTRDVQQAARALGQNLIVLSGGSDESIEASFVTLSQKRV
                     AALLIGGDPFFDTRRDRLIALALQYRVPAIYQFREYALAGGLMSYGASITDMYHQVGL
                     YVGRVLKGEKPADLPVMQVSKFETVINLKTAKALGIKISDNLLSLADEVIE"
     gene            2791..>3516
                     /locus_tag="VM910_20775"
     CDS             2791..>3516
                     /locus_tag="VM910_20775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI64994.1"
                     /translation="MHGAAIKRRTFISLLGAVAAPFIAIAFTGVVITSAQTQEFPSKN
                     VTLMMPYAAGGPGDTLTRIIGQGMGKILGRQFLVENVAGAGGTIGTAKVAASAPDGYS
                     LLVMHFGHAANTALYRNLRYDPIKDFEPIGMIAESPMAFVARKDFPADNFKDFVAHVK
                     TNREKVVHGHAGIGSASHLCGLLFLNTIDATVTIVPYKGTGPALNDLIGGQFDFMCDQ
                     TLNVFQPLQGGLIKAYAATTKKRL"
BASE COUNT          669 a         1024 c         1106 g          717 t
ORIGIN      
        1 gtctgaaaat gcagcaaggg ggttacgggt gtcggtcacg accattcctc cggttgcgaa
       61 tgactggtcg cgatatggca ctggcgcaag tccagctcta ctggccggat ggccaggtca
      121 tgccgtccgc catcgtcaca gcaaccccgt ggcagcttgg atcatccgcc aagcgcagcg
      181 atgctcgcga tcaacaggaa gaccgcgtcg gcactgacaa gcagcatcct gatccagtgt
      241 tagtcatcct ccttggttgt ggaggaggac catggcgtgc ggactgccta agcggccgca
      301 tttacagtag ctttccgggg cggtgcggac ataggctcgg gctcggggcc ggtcggggcc
      361 gctgctcatg acccgctgcg gaagtcgagc gcccgctttt gctgtgacgc acaatcctcc
      421 ctattcaaag atgtggtagg gtgtagtcct cggtttgagg gcaccatgag acggcggcaa
      481 ttcattacgc ttctcggcgg cgcagcggcg ttgccgctcg cggcgcgtgc gcagcagccg
      541 gcgaagatgc tgcgagtagg cactgttgcc ggcaccccga aatcatcacc gcagtgggta
      601 gctttcgagc ggcgcatggg tgagctcggt tatcaggaag gcaaaaattt ctccttcgat
      661 tttctgcagg ctgcgagcgc agacgaggac gaagtcctct accggaagct cgcggtgcac
      721 atgcccgatg ttatccttgc tattggcccg gaagtcggcc tgaagtcggc gctcgccgtt
      781 acgcgcacgc taccgatcgt gatgatcgct atcgactacg atccgttcgc tcgcggctac
      841 gtgaccaatc tggcgcggcc ctccggcaat gttaccggcg tggttttcca gcagatcgaa
      901 ctggcagcaa agcggattca gctcatcaag gatgcctttc gggacaggcc ggccgcgacc
      961 atgttctggg accgcttgtc tgcggatcaa tggcaagcag cgcagagtgc cgccgccacg
     1021 ctgggcttgc agctttctgg gattgaactg cgcgagttgc cctatgacta tgacgcggcg
     1081 ctcgatcagg caccgctcga tcatcgcgac atactcatag tgccaacgtc gccgttcttt
     1141 tttcgcgatc gcgcgcgcct tgccgacttt gcgcttcggc atcggatcct atccatgttt
     1201 gtcttccgcg aatgggtgga tgcgggcggc ctgttgtgct acgggccgag tatcacagcg
     1261 ctcttcgggc gcgttgctga gttcgttgat cggatcgccc gcggcgccaa gcccgccgat
     1321 ttgcctatcg agcagccgac gaagttcgag atggtggtga atctgaagac cgccagggcg
     1381 atcggtatcg atctgccgac ggcgattctg ctgcgagccg acgaggtgat cgaataacgc
     1441 tgctcttgct gcggtgcatt aagacgcttt tggcacaaag cggacgtatc gaggcgaggt
     1501 cgcgcatgtc cgctttgggg ggcaaagcag acatggtcta cgtgacggca tgccgcttct
     1561 gacccaaagc ggacaaaggc agggttgaaa tcccgcagtg cagcggcatt gtgcgtgcta
     1621 tcatttgctg gcagcgcgga agggtgggta gcgccccacg ccgcccgaca ggggaggaag
     1681 tccccatgtc aagcatcaag cgacgtgagt tcattaccct gctcggcggc gcggccgcgt
     1741 ggccgctcgc agcgcgcgcg cagcaatcgg cgccgccggt gatcgggttt ctgagttcac
     1801 ggtccgctga tgattccgcg cgcgtcgtcg ccgcctttcg ccaaggcctc gctgagacag
     1861 gctatgtcga ggaccgcaat gtggtgatcg agttccgctg ggcagagggc cagttggatc
     1921 ggcttcccgc gctggcaagt gaactggttc accggccagt tgcggtaatt gctgctcttg
     1981 gcgagtctgg atacgctgca aaggcagcca ccactaccat tccaatcgtc ttgggcagtg
     2041 gaggcgatcc cgtcgagctg ggccttgtca ccagccttaa ccggcccggc ggcaacgtta
     2101 cgggtgccac gtttctaacg gctcagctcg gagcaaagcg cctggggttg ctgcgagatc
     2161 tcgttcctgg tgcggacgtg gtcgcgctgc ttataaatcc aaacaccgct gtcgggcggg
     2221 tgcaaacaag ggacgtgcag caagcagcgc gcgcactggg acaaaacctt attgttctca
     2281 gtggcggcag cgatgagagc atcgaagcaa gttttgttac gcttagccag aagcgtgttg
     2341 ctgcgctttt gatcggtggc gatccgtttt tcgatactcg gcgcgaccga ctgatcgcac
     2401 tggcgttgca atatcgcgtg ccggcgatct atcagttccg cgaatatgca ttggccggcg
     2461 gtctgatgag ctacggcgct agtatcaccg acatgtatca ccaagtcggc ctctatgtcg
     2521 gtcgcgttct caagggcgaa aaaccagccg acctgccggt catgcaagtg agcaagttcg
     2581 agacggtgat caacctcaag accgcgaagg cgctcggcat caagatctcc gacaacctgc
     2641 tctcgctcgc tgatgaggtg atcgagtgag tgcaaagttg aggcgccgcg agttcatcac
     2701 gctgctcgcc ggcgcggccg cgtggccgct catgtcacag gcgcaggagg cttgatgacc
     2761 tgaattgaaa tcggaactgg gtgatcccgc atgcacgggg cggcaataaa gcgacgcacg
     2821 ttcatatcgc ttctcggcgc cgtggcggcc cccttcattg caatcgcctt cacgggcgtt
     2881 gtcatcacaa gtgcacaaac gcaggaattt ccgtcgaaga acgtaacgct gatgatgccc
     2941 tatgccgcgg gcgggcccgg agacacgctg acccggatca tcggtcaggg aatggggaag
     3001 atcctgggca gacaattcct agtggagaac gtggccggag cgggtggcac gatcggcacg
     3061 gcgaaggtcg ctgcgtccgc gcccgatggc tactcgctgc tggtgatgca ctttggccat
     3121 gcggccaata ccgcgctcta ccgcaacctc cgctacgacc cgattaagga ttttgagccg
     3181 atcgggatga tcgcggagtc gcctatggcc tttgtcgcaa gaaaggattt cccggccgac
     3241 aacttcaaag attttgtcgc gcatgtgaag acgaacaggg aaaaggtggt gcacggacac
     3301 gccggaatcg gctcggcctc gcatctttgc ggcctcttgt tcttgaacac gatcgacgcg
     3361 acggtcacca tcgttcccta caagggcacc gggccggcac tgaacgacct catcggcggc
     3421 cagttcgatt tcatgtgtga tcagacactc aatgtattcc agccacttca aggcggactg
     3481 atcaaggcct acgcggcgac gaccaagaag cgtctc
//
LOCUS       DATLJT010000901         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3964894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000901 DATLJT010000000
VERSION     DATLJT010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3964894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VM910_20780"
     CDS             <1..513
                     /locus_tag="VM910_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVI64995.1"
                     /translation="MPVVFFVDPALASDPETRDVGTITLSYTFFRSANPDNAKDLSRF
                     TATSEPDPVHGQQLFSERCAACHGLDANKSGPMLGGVFRRKAGSAPGYNYSPALGGAG
                     IRWSADNLDRWLTDPGKFLVGTRMPVRVPDASARRDIIAYLQKESRHTGDAPEPRSVA
                     QHSSDRSRKQ"
     gene            529..1323
                     /locus_tag="VM910_20785"
     CDS             529..1323
                     /locus_tag="VM910_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI64996.1"
                     /translation="MASKITVRPFIAWSILPLLLIIAVGAWFALGPGLRLGHRSATLS
                     ARMPKDEFEQRIQAYLLDHPEVIVESLNRMEERQRTSAETEVQAIIQSRADELFRDVD
                     SPVGGNPNGNITLVEFFDYNCPYCRQMVPVMTQVESADSQLRVVYKEFPILGPNSKLA
                     AKAALAAHRQGKYLAFHKALFQVRGTVDPSKVTEVAASVGLDVDRLKADMDDPAIAAL
                     IEKNLALAQALRIDGTPGFVARRQVLRGAIDLKALQGFIQEARGSP"
     gene            1436..1936
                     /locus_tag="VM910_20790"
     CDS             1436..1936
                     /locus_tag="VM910_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64997.1"
                     /translation="MAGALAIGAVDAASAQGMQDRMGTGMMQGGPSQGAGGGMMRGGM
                     GSSATEPGMNQCSMMHGDMGSGMMGQHMGGGMMMGPGMMQSGSRPGMGALFGSRVTPV
                     MNLSLEDVRGYLGLQLQRLNNKRLKIGDLKSDDGTITADIVTVDNSLVQRLKIDRHTG
                     AIQYEN"
BASE COUNT          465 a          651 c          697 g          384 t
ORIGIN      
        1 atgcccgtcg ttttcttcgt cgacccggcg ctggcgagcg atccggaaac gcgcgatgtc
       61 gggacgatca cgctgtccta cacgttcttc cgctcggcga atccagacaa cgccaaagac
      121 ctttcccgtt tcacggccac ctcggaacct gacccggttc atggccagca gctcttcagc
      181 gagcggtgcg ccgcgtgtca tggcctcgat gcgaacaaga gcggccccat gctcggcggc
      241 gtcttccgcc gcaaggccgg ctcagctcct ggatacaact attcccctgc gctcggtggc
      301 gccggcatca gatggtccgc agacaatctc gatcgatggc tgaccgatcc cggcaagttc
      361 ttggtcggaa cgagaatgcc ggtccgcgtg ccggatgctt cggctcggcg agacatcatc
      421 gcctatctgc agaaggaaag ccgccacacg ggcgacgcgc ccgagccgag atcggttgcg
      481 caacactcat ccgatcgatc tcggaagcag tgagagaggg gctgaggaat ggcatctaaa
      541 atcacggttc gacctttcat cgcgtggagc attttgccgc tgctcttgat tatcgcggtc
      601 ggcgcatggt tcgcgttggg cccgggactc cgcttgggac atcggagcgc gactctttcg
      661 gcacgcatgc cgaaggacga attcgagcaa cggatacaag cctatcttct ggatcatccc
      721 gaggtgatcg tcgaatccct gaatcggatg gaggaacgcc agcgcacgtc tgcagaaacg
      781 gaagttcaag cgatcattca gagtcgcgcc gacgaattgt tccgcgacgt cgacagcccg
      841 gtcggcggca atcccaacgg caacatcacg ctcgtcgaat tcttcgatta caactgcccc
      901 tattgccgac agatggtacc ggtgatgacg caggtggaga gcgcggactc ccagctacgc
      961 gtcgtctaca aagagttccc gatcctcgga cccaattcca agcttgcggc caaggcggcg
     1021 cttgcggccc atagacaggg caaatacctc gcgtttcaca aagccctttt tcaagttcga
     1081 ggcaccgtgg atccgagcaa ggtcacggaa gtcgccgcct cggtcggcct cgacgtggat
     1141 cgactgaaag ccgacatgga cgatcccgcg atcgcggcgc tgatcgagaa aaatctagct
     1201 ctcgcgcagg cactccgcat cgatgggacg ccggggttcg tggcccgtcg ccaggtcctt
     1261 cggggcgcga tcgacctgaa agcacttcag ggattcatcc aggaagcaag aggcagcccg
     1321 tgatggacca ggcatcgctg gcaggctcag ataacggaat gaaacaaagg ttcgttaacg
     1381 gctagatcaa gaggagaagc acatgaagac aagggccttt cttttggtgt cagcgatggc
     1441 gggcgcgctc gcgatcggtg cggttgacgc ggcgagcgca caaggcatgc aagacaggat
     1501 ggggactggc atgatgcagg gtgggccgag ccagggggct ggcggcggga tgatgcgggg
     1561 cgggatgggt tcgtcggcca cagagccggg catgaatcag tgcagcatga tgcatggcga
     1621 catgggctcc gggatgatgg gacagcacat gggcggcggc atgatgatgg gccccggcat
     1681 gatgcaaagc ggctcaaggc ccggcatggg tgcgctattc gggtcgcgcg taacgccggt
     1741 catgaatcta tcgctcgaag acgtgcgcgg ctatctgggc ttgcaacttc aacgcctgaa
     1801 taacaaacgt ctgaagatcg gggacctcaa aagcgacgac gggacgatca ccgccgatat
     1861 cgtcaccgtc gacaactcgc ttgtgcagcg gctgaagatc gaccggcaca ctggcgcgat
     1921 ccagtatgag aattgaacac acgcggcagc caggtggtcg cggtgcgctt tgcacgcagg
     1981 ctgactgcct ccgcccgacc cgaagggccg cgacgcccct tctaagtgaa agcggcaaag
     2041 tcggccgaca cgagtgagag gagcgaggca tgaaaacagc cactcttgac gttggcggca
     2101 tgctgtcctt gctggactac caagccgtcg agaagcagct cggctggata cccggggtcc
     2161 aacgcgcgac cgccagcgtc gcctcgaaca gcgtgac
//
LOCUS       DATLJT010000902         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3506217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000902 DATLJT010000000
VERSION     DATLJT010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3506217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VM910_20795"
     CDS             <1..1332
                     /locus_tag="VM910_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64998.1"
                     /translation="LRAAGIGASVGVAAIVAVAVVVIPWYTARLTEYDRTDGEIERLL
                     TLNRSEPKTAVRKAPTVLGNVAAYLWQQNQLDHLTALLQRSAPLITEQYRMDQRVGAV
                     MPQVGSDVPWPIELRYNPDRFLDEGRLRYEWRIIAARLMDTWGIPAPAALKLTEDNKI
                     PVNDLIVTAGELRKRIEVSTLPWFVLVSEDGMPERLQAWFDTHKAQWARVDVLEDDGP
                     WWLVPHWTQPLFDAAGHPALAREALIALAVGQALTSQPELALNPQNVAYRLSRLEGAG
                     FKRTVAEALASRGGVDGLVEDLRALVARGYPLVNLEYVIDSLAGYPRSQFSSAVVAEQ
                     VEKDQTAATSRVGARLAGAHSAPAAEAAEPSRTEDVVPYRDTADRIARQPPVRVYLGE
                     DLVSYFATSEGELHPHILEELDDLRGELFKRFGIVVPGVRFVRRAGTRRAD"
     gene            1356..2234
                     /locus_tag="VM910_20800"
     CDS             1356..2234
                     /locus_tag="VM910_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI64999.1"
                     /translation="MLTQSWADKDAIAVEVAIPKGAVEQVLLDELRRRLLAWRTWWIT
                     ADYVDKQLEQNEPLKAWLLHRYTLTDIKSLLRGVLAPTDTELDAYSTKGLGSALRQAA
                     PGQSVREFDWLLGSLVFWSQAGDGLDTAKLVRALQDTQAARLASNRPPSPANPSTGIL
                     AGIEALEQRNFGLAEEQFRKAVSADRSGAIATFLAAYGNRDSVNTMADKLTQACVPFE
                     PPGRLNERRSINEQARFEIEDFLGRHQDSIADRDRASLEYCLLEHYAGHAYGNHERQL
                     RSIQDTAVRRSARAEP"
     gene            2161..3543
                     /locus_tag="VM910_20805"
     CDS             2161..3543
                     /locus_tag="VM910_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65000.1"
                     /translation="MATMSASFDLFKTPRFGGQLEPNHKYALGYWTFELSGRSFDPPA
                     DLAAAENWLASAFREWKETEQDEASAGEAFQELLSRYESRYDKLPPRWYMDMLQRLGE
                     LHPKNPYITYVLGITLSDGSDEADVKKALFWLERAREHIDGSAVSEADRPRLSAWIEW
                     AVTKYYSAKARFSSEKARQEASQEARSRLKALIENLRNTGLIRDKDWPGGQAYATLIE
                     IHQFRKEFEEAAKVLGDSRDDGLTESPDLVANRFTLLLGVGRTDEALRLADRALKMPD
                     FEREGALFLAALSQLVTDQREAEQPAREFLLTTKHQYRDYIRLMLYWYLARQGKPDQA
                     KAYLDERWRGVNPASWPARLAQGDTQVWRERLIGYYRGSVPRDEFFAPLRSREAFEAS
                     GLNRIGMEYDDIYCEAYFYDALLQAVTGDPATRSARFAKSIQDVLEKGRGDYFEYTMA
                     LYLRSLAGSR"
     gene            complement(3647..>3994)
                     /locus_tag="VM910_20810"
     CDS             complement(3647..>3994)
                     /locus_tag="VM910_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HVI65001.1"
                     /translation="RRRLDAEAPTHFTAPSGSAVPIDYEAQEGPKLAIRVQELFGLDR
                     HPSVAAGRVPLVVELLSPAHRPVQVTRDLPGFWRGSYAAVKAEMKGRYPRHPWPDNPL
                     SAVPTRRAKPRSS"
BASE COUNT          768 a         1229 c         1288 g          709 t
ORIGIN      
        1 ctgcgcgccg cggggatcgg tgcgagcgtc ggtgtggcgg ccatcgtcgc tgtggcagtc
       61 gtggtcatac cttggtacac ggcacgtctc accgagtacg atcgcactga cggcgagatc
      121 gagcgcctgc tcacgctcaa cagaagcgag cccaagactg cagtgcgaaa ggcgccgacg
      181 gtgctcggca acgtcgccgc ctatctttgg cagcaaaacc agctcgacca cctgaccgcg
      241 ctgctgcagc gctccgcgcc gttgatcacg gagcagtacc gcatggacca gagggtgggc
      301 gccgtcatgc cgcaagtcgg cagtgacgtg ccctggccga ttgagctgcg ttacaacccg
      361 gatcgctttc tcgatgaggg ccgccttcgc tacgaatggc ggatcatcgc agcgcgatta
      421 atggacacgt ggggtatccc ggcaccggcc gcgttgaagc tcactgaaga caataaaata
      481 ccggtgaatg atctcatcgt gaccgccggg gagctaagga aacgcataga ggtatccacg
      541 ctgccgtggt tcgtccttgt ctccgaggac ggcatgccgg agcgcctgca agcgtggttc
      601 gacacgcaca aggcgcagtg ggcacgcgtc gatgtcctag aagatgacgg gccgtggtgg
      661 ctggtgccac actggaccca gccgctgttt gatgcggccg gccacccggc attagcgcgc
      721 gaggcgttga tcgccttggc ggtcggacag gccctgacct cgcagcctga actggcactt
      781 aacccgcaga acgtggccta ccggctgagc cgcctggaag gcgctggttt caagcgcacg
      841 gtcgccgagg cgctggcctc ccgcggtggc gtcgacgggc tcgtcgagga tttgcgcgcc
      901 ctcgtcgcac gcggctatcc gctcgtgaac ctggaatacg tgatcgacag cctagcgggt
      961 tatccgcggt cgcaattctc gtccgcggtc gttgctgaac aggtcgagaa ggaccagacc
     1021 gccgcaacgt cgcgggtcgg tgcccggttg gccggcgcgc atagtgcccc agccgccgaa
     1081 gccgccgagc catcccggac cgaggatgtc gttccttacc gcgacacggc cgaccgcata
     1141 gccaggcaac ccccggtgcg cgtttacctc ggcgaggatc tggtatctta ctttgccacg
     1201 tctgagggcg aattgcaccc gcacatcctg gaggaactgg acgatcttcg cggcgagtta
     1261 ttcaagcgtt tcggcatcgt ggtgccggga gtgaggttcg tccgtcgagc tgggacccgg
     1321 cgggcagact agcttccaac gcttttcgca tcgaggtgct gacccagtca tgggctgaca
     1381 aggacgcgat cgcagtcgag gtcgccattc ccaagggtgc cgtcgaacag gtcctcctcg
     1441 acgagttacg ccggcgcctg ctcgcatggc gtacttggtg gatcacagct gactatgtgg
     1501 acaaacagct ggaacagaat gagccgctca aggcctggct actccaccgg tacacgctga
     1561 ccgacatcaa gtcgcttctg cgcggcgtgc tggcgccgac cgataccgag cttgatgcct
     1621 acagtaccaa gggcctcggc agcgccctgc ggcaggccgc tcctgggcag tccgtgcggg
     1681 agttcgactg gctgctcggc agccttgtgt tttggtcgca ggctggcgat ggtctcgaca
     1741 ccgcgaaact cgtgcgggca ttgcaagaca cccaggcggc gcgactcgca tccaaccggc
     1801 caccttcgcc cgccaacccc tcaacgggaa tcctcgctgg aatcgaagcc ctcgagcaac
     1861 gaaactttgg gcttgccgag gagcaatttc gtaaggccgt gtccgcggac cgcagcggcg
     1921 caattgcgac ttttctggcc gcctacggaa atcgtgacag cgtgaatacg atggctgaca
     1981 agctgaccca ggcctgcgtt ccttttgaac ctcctggtcg gctcaatgag cgtcgctcga
     2041 taaacgagca agcgcgcttt gagattgaag atttcctagg ccgccatcag gactcgatcg
     2101 ccgaccggga ccgggcaagt ctggagtatt gcctgctcga acactatgcc ggccacgcct
     2161 atggcaacca tgagcgccag cttcgatcta ttcaagacac cgcggttcgg cggtcagctc
     2221 gagccgaacc ataagtatgc cctagggtac tggaccttcg agctgagtgg ccgaagcttt
     2281 gatccgcccg cggatttggc ggcggcggaa aattggctcg ccagcgcatt tcgcgagtgg
     2341 aaagaaacag aacaggacga ggccagcgct ggagaggcgt ttcaggagct gctgagccgt
     2401 tatgagagtc gttatgataa attgcctccg cgctggtaca tggatatgtt gcaacgcctg
     2461 ggggaactac acccgaaaaa tccctatatc acgtatgtgc ttggaattac gctttccgac
     2521 ggcagcgatg aggccgacgt caagaaggcg ctcttttggc tcgagcgggc gcgagaacac
     2581 attgatggat ccgctgtgtc agaagccgac aggccccggt tgagcgcctg gatcgagtgg
     2641 gctgtcacca agtactattc ggccaaggca cggtttagtt ctgagaaagc gcgccaagag
     2701 gcgtcgcagg aggcaaggag tcgtctgaag gcattgatcg agaacctcag gaacaccggc
     2761 ctcatacgcg ataaggattg gccgggcggt caagcctacg ccacgctcat cgagatccac
     2821 cagttccgca aggaattcga ggaggcggcg aaggtcctcg gcgactcgcg cgacgacggc
     2881 ctgaccgaga gccccgacct cgtggccaat cgattcaccc tccttctcgg ggtcgggcgc
     2941 accgatgagg cgttacgact tgccgaccga gcgctcaaga tgccggactt tgagcgtgag
     3001 ggcgccttgt tcctcgcggc gctgtcgcaa ttggtgaccg accagcgtga agcggagcaa
     3061 ccggcccgtg aatttcttct cacaacgaag caccaatacc gggactatat acgcctcatg
     3121 ctctattggt acctcgcccg acaagggaaa cccgatcagg caaaggccta cctggacgag
     3181 cgttggcgcg gcgtcaatcc ggcttcgtgg ccggcgcgcc ttgcacaggg cgacacccag
     3241 gtgtggcgcg agcgattgat cggctattac cgggggagcg tcccacgcga cgagttcttc
     3301 gcgccgctgc gcagtcgcga ggcgttcgag gcctctgggc tcaaccggat cggcatggag
     3361 tacgatgaca tatattgcga ggcctatttc tacgacgccc tacttcaggc ggtcacgggc
     3421 gatcccgcca cccgctcggc acgtttcgcg aaaagcatcc aggacgttct cgaaaagggc
     3481 cgcggagact attttgaata cacgatggca ctgtacctgc gctcactagc gggtagccga
     3541 tagagcctct tccgcgaggg ggaagggcgt gagcggcacc agccgcgccg tgcccggcct
     3601 agtaaccaga atacagaaat ccgaagtgcg cttgcgcaat tgcgcattaa gacgagcgcg
     3661 gcttggcgcg gcgcgtgggc acggcagaga gtggattgtc gggccaggga tggcgcgggt
     3721 agcggccctt catttcagct ttgaccgcgg cgtagctgcc gcgccaaaag ccgggcagat
     3781 cgcgcgtcac ctgcaccggc cgatgcgccg gcgacaacag ctcgaccacg agcggcacgc
     3841 gccccgctgc gacgctggga tggcggtcga ggccgaacag ctcctgcacg cggatcgcaa
     3901 gcttgggccc ctcttgcgcc tcgtagtcga tgggcaccgc ggagcccgac ggcgcggtga
     3961 aatgcgtggg agcctcggcg tcgagccggc ggcg
//
LOCUS       DATLJT010000903         4046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4195142,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000903 DATLJT010000000
VERSION     DATLJT010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195142"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..863)
                     /locus_tag="VM910_20815"
     CDS             complement(426..863)
                     /locus_tag="VM910_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65002.1"
                     /translation="MFAGDAIIINIVGGRFVGHSLKYFIAADISAHDQFLMESPQVKG
                     DKVEWTRSVTAGFYATLGVAPVQFAFEAIVQRGKVKSIVAHLPTAEIARIEAACRARP
                     KEPLIYGQPCGEFVQYLKEHTQSVLRDARAQAQPGGLQVAASK"
     gene            complement(2387..2575)
                     /locus_tag="VM910_20820"
     CDS             complement(2387..2575)
                     /locus_tag="VM910_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65003.1"
                     /translation="MRTIVVLGTTVLLTLAVIVGWVAQTTPYTDQAKAVTAPAAPIDP
                     FELMKKSNGLVHQQYDAF"
     gene            3336..>4046
                     /locus_tag="VM910_20825"
     CDS             3336..>4046
                     /locus_tag="VM910_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI65004.1"
                     /translation="MEGRLGAVQALNSMTDDAVSFGRFRFDLRRRELFRDESSVRLGG
                     RALDVMHALAAAKGDVVSKTDLLARVWPGMAVGENNLQVQISLLRKTLQEDTSGESYL
                     VTVPGRGYRLVGLDTLAQGPALPDKPSIAVLPFENRSDDPGQDYFADGIVEDIITALC
                     RIRWLFVIARNSSFSYKNRVVDIKQVGRELGVRYVLEGGVRKDTQRVRITAQLVDAAS
                     GMHLWADHFDSGIENIFDL"
BASE COUNT          799 a         1208 c         1170 g          869 t
ORIGIN      
        1 gcggcgctgc tcggaattcg cggcccgacg agatgaccca cgtcttccag gttccggctt
       61 tcggctcgat ccgatcgcca gccacctgct gggcaagtgc tttgggcatc agcagcaacg
      121 cgcaaagtcc gctcagaagg atgctagtta ctgcaaaacg cgttttcata gctttcctcc
      181 ttggtttggt gagcgccctt ggccgcgatc gcttgctgtc ataaactatt ggggccgaag
      241 cgccctgtgc atgaaccact tcacatcggt ccgcgccgaa aagcgtgatg aactcgcgtc
      301 gcctcattcg atcacctcat cggcaagtgc gagcaatttg tccggaatct gaagcccgag
      361 cgccttggcg tgttgaggtt gatccgaatg aagctgattg aatgccagaa ccgcggacag
      421 ccgcgttatt tcgatgccgc cacttggaga cctccgggct gcgcttgggc tctggcgtcg
      481 cgtaacactg attgcgtgtg ctccttgagg tattgaacga attcaccgca aggttgaccg
      541 tagatcaggg gctccttcgg ccgagcccga caggccgctt cgatccgcgc aatttcggcc
      601 gtaggaagat gcgcgacgat ggatttgact tttcccctct gaacaatcgc ttcgaacgca
      661 aattgtaccg gtgcgacgcc aagcgttgca tagaacccag ccgtcaccga cctagtccat
      721 tcgactttgt ctcctttgac ctgcggcgac tccatcaaaa attgatcgtg agccgagata
      781 tctgctgcga tgaaatattt caggctatgg ccgacgaacc ttcccccgac gatattgatg
      841 atgatcgcgt cgccggcgaa caaggaaagc gctgccttaa ggtcgcccac ggtctccacc
      901 tccatcaatt tcttcagcac tgccaaagac tcgggctccg acctatccga cggcccgaca
      961 tttcccgacg gaatatcgtg attgcttgac gacagctgcg cgactgcaga gcaggtgaac
     1021 gtggccgaaa gaacgacagc gagtgcgatc ttcgaagtcc acaatgacat ggaagtctcc
     1081 atctttacgc gatcggcgcg ttggggccgc cgccttgtgt cgagggaccg atgccctatg
     1141 atgctctccg tcacctcgcc accggtgtga tctgcttcac atgtcgattc gatgttttcg
     1201 cttcgaagca cctttagccg aagcgcgttg ggcgccgcgg gcgctgctcc tcgctcacgt
     1261 gctgatatcg cgcagcctga atatcgcgaa accgccgctc gagacctgcc gcccggtaga
     1321 agcccgcgcc gctcgcgcgg gtcggtaagc gtcaccctga cgtgagatgt cgtcggcata
     1381 atcggcaaag acaaaggtcc ccatcatagt gcaccgggat gttgccaatg aatttggcgg
     1441 tgtcgctgtt catctcgaac gcttttcggt tgggccgcgc accggcacgc cgtggcttgc
     1501 caaaggcgcc atccattaat cgggagtagg attgcgtgca ggatccagac aggaataaga
     1561 ccgagtccaa ttccatagat gaggaacgcg atgttgctgg tcagcgcgac aacgcggagc
     1621 ggaatgatgt ccctcataca gaacgccatt accactaagc ccgaagcgag gtatccggcc
     1681 gcgtcccaga gcctcatggc tcatactcga tgtccacgat tgcttgccgt tttgaaaagc
     1741 gcgccgcttg catcccgtgc catcgtcagt atgagccgcc gcccgcggcg cgcgagccac
     1801 atcgttgttg ccgcccaaag tcgcacctta tggtatgtcg cgctgagact tcttgcgatt
     1861 aactcgcccc taagtgtgac ggcccggcga cgataaaatt cgatgtcaat cgacccctcg
     1921 cttcgccgcc gaacttgctc cgaatgtatt tcattcatcg gtttcatcgt gaaacctcca
     1981 ctctgcgtca gcatcgttgg actgcacgac aatccgttgt ggaccgcacg tccgagaagc
     2041 ttcattcagt cttaaggcgg cgtccgagcg tgaccgcatt tggctagcat gtcccgacgc
     2101 tttcgactgt gaggcgatgc acagatcagt gccgtggtga gtcattctcc ccacgagccg
     2161 ccacagatct gcggcacgat ctgagtatcg ggcgttggac gccctcgggc cctacggatg
     2221 ctgcttgacg tcctcgtcgc tgacgcgacg gtgatgataa cgtcctcgcc gttcttccag
     2281 ttcaccgggg tcggcacctt gttgttggcc ccgagtcgcg tccacctcag catgcaggcg
     2341 ctccgttcaa ggcgaacggc acagcagact ccctttgaat aggtattcaa aaggcatcgt
     2401 attgctgatg aacgaggccg ttcgatttct tcatcaattc gaacggatct atgggagcgg
     2461 caggtgcggt gacggccttc gcctggtcgg tgtagggagt ggtctgagcc acccagccaa
     2521 cgatgactgc gagcgtgagc aaaacggtcg ttcccagcac cacgatggtt cgcatcgatc
     2581 cctccttggt cgcttgagag ttctcctgtt tcccttctga cccaccagcc cagtgcgagc
     2641 agaatgtacg cgggaaatct cttaagctct gcagttcagg accttaattg ttctgaactc
     2701 cgtgaccgac gatgcggtca ggttcggcca atttcgattc gacctgaggc ggcgcgagct
     2761 gttccgcgac gaatcctccg tgcggctcgg cggccgggcg cgcgacgtct tgcacgcttt
     2821 agctgccgcc aagggagatg tcgtcagcag gacggagcta ctcgtgcgag gccagggaac
     2881 ggccgttggc gagaacaatc tgtcggtcca gatctctctg ttgcgaaaga cgcttcaaca
     2941 agacacgagc ggcgagagtt acctagttac cgtctcgggc catgactatc gacttgtcgg
     3001 ccacagtcgc ccgatcgcaa gccgccgata ttgttgcaaa acgattttgc ggcgcggaca
     3061 ggtgagtggg cgtgtgacca agttcacata atcaccgcca aaacgggata tcatttctgt
     3121 tgatcaaagg ctgaggccca tggcgcgctt tccaatgaat gtcgccatcg gcaagacgaa
     3181 aattcgccgc tgcgctcggc ttcgcggtgg tcacatagtt gctctcgtcc cgcgcacagc
     3241 agccagcgcc ggtgatcggg tttgtcagcg gcgggtcgcc ggactcgtgg gcgatcgttc
     3301 gcgcgcgttc cgccgacctt gaacgaggcc ggctagtgga aggaagactt ggcgccgtcc
     3361 aagccttgaa ctccatgacc gacgatgcgg tcagtttcgg ccgatttcga ttcgacctga
     3421 ggcggcgcga gctgttccgg gacgaatcct ccgtgcggct cggcggccgg gcgctcgacg
     3481 tcatgcatgc tttagctgcc gccaaaggcg atgtcgtcag caaaacggac ctactcgcgc
     3541 gagtgtggcc tggaatggcc gttggcgaga acaacttgca ggtccagatc tctctgttgc
     3601 gaaagacgct ccaagaagac accagcggcg aaagctacct agttaccgtc ccgggccgcg
     3661 gctatcgact tgtcggcctc gatacgctcg cgcaaggacc ggccctcccg gacaagcctt
     3721 cgatcgccgt cttgcctttc gagaacagga gcgatgaccc gggacaggac tatttcgcgg
     3781 acggaatcgt cgaggacatc atcacggcgc tctgccgcat ccgctggctg tttgtgatcg
     3841 cgcgcaattc gagctttagc tacaagaacc gtgtggtcga tataaagcag gtgggtcggg
     3901 aacttggcgt tcgttatgtg ctcgaaggcg gcgtgcgcaa ggacacacag cgcgtgcgca
     3961 tcacggctca gctggtcgat gcggccagtg gcatgcatct ctgggcagat cactttgaca
     4021 gcggcatcga gaacatcttc gatctg
//
LOCUS       DATLJT010000904         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1668506,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000904 DATLJT010000000
VERSION     DATLJT010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668506"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VM910_20830"
     CDS             <1..1051
                     /locus_tag="VM910_20830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65005.1"
                     /translation="TTIGIVSGFGELMGYGARLLSGYLGDRTGSYWAVTIVGYVLNLF
                     AVPLLALAGAWQTAVVLIVLERTGRGIRSPVRDAMLSHAASQTGLGWGFGLHQALDQL
                     GAVLGPLIVSGILFAGNGYRTGFAWLLVPALMSMTVLLAARLLFPRPHDFELAPPLAD
                     TGGLAPVFWVYIAAVACIAAGYADFPLIAYHFDKAQIMPAAWIPILFAVAMGVDGVGA
                     LALGSLFDRIGIRTMILATIVSAATAPLVFLGNFSLAVIGMACWGLGTGAQDSVMRAT
                     MVRLAPQQRRATAFGIMNAIYGVAWFAGSVLLGLLYDRSISAVVLASTLLQAAALPIF
                     AWLVAREARPRSRLA"
     gene            1063..1482
                     /locus_tag="VM910_20835"
     CDS             1063..1482
                     /locus_tag="VM910_20835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65006.1"
                     /translation="MFRKILVANDGSPGGQAALSGAIDLALQCGSELHMICIEEIPRF
                     AATIDEIEEEKEEADLRFTSVIRRAKELAAQKGVSLETHLLAGHPVPSIVEFLRAGTF
                     DLLVVGFMGHSAIYNRLIGSTTDRLVDHAPCAVLVIK"
     gene            complement(1908..2909)
                     /locus_tag="VM910_20840"
     CDS             complement(1908..2909)
                     /locus_tag="VM910_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65007.1"
                     /translation="MTMLQTRRRFLATVSGAGAAGLVRAPRIQAAEGPLETTAVRLPK
                     VPALCLAPQYAADELLRAEGFTDIRYVEMAHSTLMYEAMGRGEVDVGLNLAISHVRAI
                     DARAPIAVIGGVHGGCYELFAGSGTRSIIDLKGKSVGVLALGSSPHAFLSVMAANVGL
                     DPVKDVRWVTSGDPLELFTQGKIDAFLGFPPEPQELRNRGVGHVIVNTAIDRPWSQYF
                     CCMLAGSQDYIRKYPVATKRVLRAVLKAADLCAADPARTARRLVDGGFASRYDYALQT
                     LRDVAYDRWREYDAEDTIRFYALRLHETGFIKSSPQKIIAEGTNWRFLDELKRELKA"
BASE COUNT          506 a          989 c          962 g          621 t
ORIGIN      
        1 gacgacgatc ggcatcgttt ccggtttcgg cgagttgatg ggctatggcg cccgcctcct
       61 ctccggctat ctcggcgacc gaaccggcag ctactgggcc gtcacgatcg tcggctacgt
      121 gctgaacctg ttcgccgtgc cactcttggc gctcgccggc gcctggcaga ccgcggtcgt
      181 gctgatcgtc ctcgagcgga ccggccgcgg catccgctcg ccggtgcgcg atgcgatgct
      241 gtcgcacgcc gcgagccaga ccgggctcgg ctggggcttc gggctgcatc aggcgctcga
      301 tcagctcggc gccgttctcg ggccgctcat tgtcagcggc atcctgttcg ccggcaacgg
      361 ctaccggacc ggcttcgcct ggttgctggt tccggcgctc atgtcgatga cggtcctgct
      421 cgccgcccgg ttgctgtttc cgcggccgca cgatttcgag ctcgcgccgc cccttgccga
      481 taccggaggc ctcgcgccgg tattttgggt atacatcgcc gcggtcgcct gtatcgcggc
      541 cggctatgcc gacttcccgc tgatcgccta ccattttgat aaggcgcaga tcatgccggc
      601 ggcgtggatc ccgatcctgt ttgcggtcgc gatgggcgtc gacggagtcg gcgcgttagc
      661 cttgggctcg ctgttcgacc ggatcggcat ccgcacgatg atcctggcga cgatcgtgtc
      721 ggccgccacc gcgccgctcg tcttcctcgg caacttttcg ctcgcggtca tcggcatggc
      781 gtgctggggc ctcggcaccg gcgcgcagga ttcggtcatg cgcgcgacga tggtgcgcct
      841 cgcgccacag cagcgccgcg ccaccgcctt cggcattatg aatgcgatct atggcgtcgc
      901 ctggttcgcc ggcagcgtcc tgctggggtt gttgtacgac cgctcgatct ccgccgtcgt
      961 tctcgcttcg acattgctgc aggccgcagc gttgccgatc ttcgcctggc tcgtcgcgcg
     1021 cgaggcacgg ccgcgaagcc ggttggcctg aacgagaagc caatgtttcg gaagatcctt
     1081 gttgcgaatg acggatcgcc cggcgggcag gcggcccttt ccggcgcgat cgatctggcg
     1141 ctccagtgcg gcagcgaatt gcacatgatt tgcattgagg agatcccgcg ctttgccgcg
     1201 acgatcgacg agatcgaaga ggaaaaagag gaagctgacc tccgcttcac gagcgtcatc
     1261 aggcgcgcca aagagctggc cgctcagaag ggagtgtcgc tcgaaaccca cctgctggcc
     1321 gggcatccgg ttccgagcat cgttgagttt ctgagggctg gcaccttcga tcttttggtg
     1381 gtcggcttca tggggcattc ggccatttac aaccggctca ttggcagcac caccgaccgt
     1441 ctcgtcgatc acgccccctg cgctgttctc gtgatcaagt gatgtgttgg tgctcgctgg
     1501 cggggtgcta caccctttac gcgcgcgcgt gcgaagggtg taactcaacg taggtcaaga
     1561 agaccctgca cggcgcgtcg tctgtgatgt agatctcgga ccgctttcga gaccgacaac
     1621 ggtcgctcag ccttggcggc gggaactggc cttcatgccc ccaatccggt cgttgtccga
     1681 ggcgagcccg aagcgctcag gaggctggga agccgccatt cgctcccccg ccatcgatta
     1741 gccgaggtgc gccataagtg attagcaaaa accgatttgg cctttgcgct tggcgtactg
     1801 ccgatgaagg aaataggtct acgccattgg gtagcccatt cccaagattt cgtcgacgct
     1861 tgacttgctc gccctccgcg cgtgaaaccc gggttagcac tcggtgttca cgccttcagc
     1921 tcgcgcttga gctcgtccag aaaacgccaa ttggtgccct cggcgatgat cttctgtggg
     1981 ctcgacttaa tgaagcccgt ttcatgcagc cgaagggcgt aaaagcgaat cgtgtcctct
     2041 gcgtcgtact ctcgccacct gtcgtacgcg acgtcgcgca atgtctgaag cgcatagtcg
     2101 taccgcgagg caaaaccgcc atcaacgagc cgtctcgcgg tgcgggccgg gtcggcggcg
     2161 cagagatcag cggccttcag cacagcgcgc agcacccgct tcgtcgcgac cgggtatttg
     2221 cggatgtagt cctgtgatcc cgccagcatg cagcagaaat attgcgacca cgggcggtcg
     2281 atcgcggtgt tgacgatcac gtgaccgaca ccccggttgc gcaactcctg aggttcaggc
     2341 ggaaaaccca agaaggcatc gattttgccc tgcgtgaaaa gttcaagcgg gtcgccactg
     2401 gtgacccagc gcacatcctt gacggggtcg agcccaacat tggcggccat tacgctcaag
     2461 aatgcgtgcg gcgaagagcc caatgccaag acgccgacac ttttgccctt caagtcgata
     2521 atgctgcgag tacccgagcc ggcaaacagc tcatagcagc ccccatgcac gccgccaatc
     2581 accgcgatcg gtgctcgggc atcgatcgcc cgaacgtgcg agatcgcaag gttcagaccg
     2641 acatccacct cgccccgccc catggcttcg tacatcagcg tggagtgcgc catttcgacg
     2701 tagcggatat cggtgaagcc ctcggcgcgc agcagttcgt ccgcggcata ttgtggcgca
     2761 aggcacaggg ccggcacctt aggaaggcga acagcagtcg tttcaagggg cccttccgcg
     2821 gcttgtattc gtggtgcccg aacaaggccc gcggcaccgg cccctgagac ggtggccaag
     2881 aagcggcgtc gggtctgcag catggtcatc gagtccccct ttccggttta cgccttcagc
     2941 tcgcgcctca attcgttgaa gaagcgccaa tcggtgttct cggcgatgat ctgctgcggc
     3001 gtcgacttga tcaggccgtt ttcatggagg cgcagcgcgt agaagcggat cgtgtcctcg
     3061 ggatcgtatt cgcgccat
//
LOCUS       DATLJT010000905         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1208457,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000905 DATLJT010000000
VERSION     DATLJT010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208457"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VM910_20845"
     CDS             <1..540
                     /locus_tag="VM910_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVI65008.1"
                     /translation="IPYYMEREAPPAPDAYAAMVKRVVHNLGCTLMFEPGRMIVGNAG
                     ILVTRVIYVKHGEAKNFVVIDAAMNDLIRPTLYEAHHDILPVVKVVGARSMVADVVGP
                     VCESGDYLALDRTLPAPKAGDLLAIMTAGAYGAVQSGTYNTRPLVPEVLVKDDQYAVV
                     RPRIDVDALIAMDRFAPWL"
     gene            complement(578..1489)
                     /locus_tag="VM910_20850"
     CDS             complement(578..1489)
                     /locus_tag="VM910_20850"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI65009.1"
                     /translation="MDIGFIGLGKMGLPMARRLIEAKHQLVVFDTRKEAVDKLIALGA
                     QAATSPRDVADRTETVMASLPSLQASLEVATAGGGVIEGRRVKRFVDLSTVGSQMAVR
                     IHDLLAKRNIVQLDSPVSGGVGGAEKGTLAVMVSGPKADFEAVKPALEVIGRVFFIGT
                     KPGSGQTMKLANNFLSATAVVATSEAVVMGVKSGLDAGVMIDVINAGSGMNTASRDKF
                     PRSILPRTFDFGFTTGLMVKDVRLAVEEMKALGLKMEVAEAVGRLWEVVIRDEGAESD
                     FTTAIKPIEKAAGVVVGGAKGGPHAAK"
     gene            1723..>2748
                     /locus_tag="VM910_20855"
     CDS             1723..>2748
                     /locus_tag="VM910_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HVI65010.1"
                     /translation="MNGVNPDPSEPSRERDGIARLKLTQALQRAKLAIAWEGAWPVLA
                     RLFTIIGLFLVVSWVGIWLALPFPARAIGLGLFLLAAFWAVWPLVRFRWPSREQGLRR
                     LDRGSGLAHRPATTLTDTLRTQDPVTLALWQAQRERTLASLKRIRAGLPSPRLRLHDP
                     WALRALVGIMLLAAYIAAGDERTVRVAAAFDWNGVLSPANIRVDAWVNPPPYTGKPPI
                     ILSAANKEAALPASGPLPVPTGSTLIVRSSGGTLDVVSGGGVTEAASTEQAPKGTNEK
                     HFTIAGDGSAHVRAPSGQPQWKFTAIPDRPPTIALAKDPERQARGSLQLSYKIEDDYG
                     VTEAQAHF"
BASE COUNT          436 a          913 c          892 g          507 t
ORIGIN      
        1 attccctatt acatggagcg cgaagcgccg cccgcgcccg acgcctatgc cgcaatggtc
       61 aagcgcgtcg ttcacaatct cggctgcacg ctgatgttcg agccggggcg gatgatcgtc
      121 ggcaatgccg gcatcctggt cacgcgcgtg atctatgtga agcatggcga agcgaaaaac
      181 ttcgtcgtca tcgatgccgc gatgaacgac ctgatccgcc cgacgctgta cgaggcgcac
      241 cacgacatcc tgccggtggt caaggtcgtg ggcgcgcgca gcatggttgc cgatgtggtc
      301 ggtccggtct gcgagagcgg cgactatctt gcgctcgatc gcacgctgcc tgcgcccaag
      361 gcgggcgatc tgctcgcgat catgaccgcc ggcgcctacg gggcggtgca gtccggcacc
      421 tacaatacgc gcccgctggt gccggaagtg ctagtcaagg acgaccaata tgcggtggta
      481 cgcccgcgca tcgacgtcga cgccctgatc gcgatggaca ggttcgcgcc ctggctgtga
      541 agcgctgacc gggggcgtgg agggatcgcc agcgaactca ctttgccgcg tgtgggccgc
      601 cctttgcgcc gccgacgacg acgccggccg ccttttcgat cggcttgatc gcggtggtga
      661 aatcggactc tgcgccctcg tcgcggatca cgacttccca caagcgcccg acggcctcgg
      721 cgacttccat cttcaacccc agcgccttca tttcctcgac ggcgagccgc acgtccttca
      781 ccatcagccc ggtcgtgaag ccaaaatcga aggtgcgcgg caggatcgat cggggaaact
      841 tgtcgcggct cgcggtgttc atgccggagc ccgcattgat cacgtcgatc atcacgcctg
      901 cgtcgaggcc ggacttgacg cccatcacca cggcttcgga ggtcgccacg acagcggtcg
      961 ccgacaggaa gttgttggca agtttcatgg tttggcccga acccggcttg gtgccgatga
     1021 agaacaccct gccgatcacc tcgagcgcag gcttgaccgc ctcgaaatcc gctttcggcc
     1081 ccgacaccat caccgcgagc gttcccttct cggcgccgcc gacgccgccg gagacggggc
     1141 tgtcgagttg cacgatgttg cgcttggcga gcaggtcgtg gatccgtacc gccatctgcg
     1201 agccgacggt ggagagatcg acgaagcgct tcacgcgccg gccctcgatc acgccgcccc
     1261 cagccgttgc aacctccagc gaagcttgca gcgacggcag gctcgccatc acggtttcgg
     1321 tacggtcggc gacgtcccgc ggcgaagtgg ccgcctgcgc gccgagcgcg atcaatttgt
     1381 ccacggcctc cttgcgcgta tcgaacacca cgagctgatg tttcgcctcg atcaggcggc
     1441 gcgccatggg aaggcccatc ttgccgaggc cgatgaatcc aatatccatg ggagtgcgcc
     1501 ctaagccctg cgccgctttg ccgctttgtc gcgcccgatc atctttgcgt ccatgacgtt
     1561 tcctctgttg ttttggcccg gcgggcgaaa cttacggggt tgccgggggt tagtcacgcc
     1621 ttcgtgttat gcgtatttcc cagcgtggga taccggataa gaacggatgc ctcactaccg
     1681 ccgttgtggt aggctgattt gccggcaaag tcggagattt tattgaacgg cgtgaaccct
     1741 gatccgtccg agccgtcgcg cgagcgagat ggcatcgcgc ggctcaaact gacgcaggcc
     1801 ctgcagcggg ccaaactggc gatcgcgtgg gaaggtgcct ggcccgttct ggcacggctg
     1861 ttcaccatca ttggactgtt cctggtggtg tcctgggtcg gaatctggct ggccttgccg
     1921 ttccccgcgc gtgccatcgg gctcggcctg ttcctgctcg ccgccttctg ggcggtctgg
     1981 ccgctggtcc ggtttcgctg gccgagccgc gaacagggtt tgcgtcggct ggatcgcggt
     2041 tcgggccttg cccatcgccc ggcaacgacg ctgacggaca cactgcgcac gcaggacccg
     2101 gtcacgctgg cgctgtggca ggcgcaacgc gagcgcacgc tggcatcgtt aaagcgcatc
     2161 cgcgccggcc tgccgtcgcc gcgcttgcgg cttcacgatc cctgggcgct gcgtgctttg
     2221 gtcggcatca tgctgctggc tgcctatatc gcggccggcg acgagcggac ggtgcgggtt
     2281 gccgcagcat tcgactggaa cggcgtgctg tcgccggcca atattcgcgt cgatgcgtgg
     2341 gtgaacccgc cgccctatac cggcaagccc ccgatcatcc tctcggccgc gaacaaggaa
     2401 gccgcattgc ctgcatccgg accgttgccg gtgccgaccg gatcgacgct gatcgtgcgc
     2461 tcgagcggcg gcacgctgga cgtggtgtcg ggcggcggcg tcaccgaagc ggcgtcgacc
     2521 gagcaggcgc ccaaaggcac caacgagaaa catttcacca tcgcgggcga tggcagcgcg
     2581 catgtgcgcg cgccctccgg tcagccgcag tggaagttca ccgcgattcc cgaccgcccg
     2641 ccgacgattg ccttggcgaa ggatcccgag cggcaggcgc gcggttcgct gcagttgtcc
     2701 tacaagatcg aggacgatta cggcgtcacc gaagcgcagg cgcatttt
//
LOCUS       DATLJT010000906         4538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4195421,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000906 DATLJT010000000
VERSION     DATLJT010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195421"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VM910_20860"
     CDS             <1..1115
                     /locus_tag="VM910_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI65011.1"
                     /translation="KSRRRSADDVERTIREHYVQGIRHFFITDDNLARNKDWEAIFDR
                     IIALRERENMNIRLMVQVDTLCHKIPNFIEKAARAGTNRVLVGLESINAANLLAAKKR
                     QNKITEYRTMLLAWKNAGAITTAGYILGFPFDTPESIREDIAIIQKELPLDILEFFCL
                     TPLPGSEDHKVLWQKGEWMHEDMNLYDGEHAVSHHPKMSKEEWEQVYRSAWDWYYTPE
                     HMETILRRGAATSCSMSRLVSFVFLFCSSVPLENAHPLQGGLLRRKYRRDRRPGMPIE
                     PIWSFYPKFAWDFVSKYSRAMRLLLWLHRTNRRLQRDPNRYMYTDLAMTPVTEDETEK
                     LELFTHSDAARQAVAHAHKIDQLTHAHTPARAEAHA"
     gene            complement(1132..1716)
                     /locus_tag="VM910_20865"
     CDS             complement(1132..1716)
                     /locus_tag="VM910_20865"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HVI65012.1"
                     /translation="MTPLAAKVIWVLGVVGWFIIRYPHDRCARRTPKLRRTDRGREIV
                     LMAISATGLGVLPFIYVVSDAPRFANYPFRPWQAWIGDAVFAASLWLFHRTHKDLGRN
                     WSVTLEVRDQHTLVTSGVYSRVRHPMYSAFWLWALAQALLLPNWIAGPAGLIGFGTLF
                     LLRVGREEALMIETFGDDYRRYMARTARILPGIY"
     gene            complement(1713..2948)
                     /locus_tag="VM910_20870"
     CDS             complement(1713..2948)
                     /locus_tag="VM910_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65013.1"
                     /translation="MIRRRHVYHLAGYDPIDAGAQYRRFARQLDVFRRTWNVDATLSA
                     LEQSNAQSRAWWSVSARAANWQVETVHEALLWDDIVRGDFTRPLPLRLFKAARAYLDF
                     IVTGTMFRYIIANQRYAGFFLFPILSVVLFAVVGWLAARLLTASLGLEGMGAAAVGLA
                     AGVAVFLVLLRWPGRRWRVQQLLDDWICANDYIHGRRPDIDARLDRFAETLLARAREA
                     AVDEIVLVGHSLGAMLVLDVVVRALARDADFGRRGATICVLTIGATIPKFALHPRAQG
                     VRGTIARVVAEPSIAWTEYQSRADTISFYKFDPVSLRHIPDDRLDGKPVIRRVQIHDM
                     LLPETFARCRHSVLRLHYRSVMANDRRAPYDYFLMVCGPVPFLEWTISPVGLLDFVAA
                     DGTYRDASRPRTVLAQPSL"
     gene            complement(2961..3845)
                     /locus_tag="VM910_20875"
     CDS             complement(2961..3845)
                     /locus_tag="VM910_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI65014.1"
                     /translation="MIRVKKIAHAVYEMPDVDKQTEYYTEVLGLTVTDKGKDAVYLAS
                     TVDHHSVVLRKGSQAKCVRLGFQIAPEDDLGAFEKHVQGHGIKTQRRKDPEPSISDMV
                     TFEDPKGTVMEVFKRDAFSGQRFPTKGIVPHKLGHVAFFVTDVKHVTKFYCDVLGFRE
                     SDWMADFFSFLRCGRDHHTINLMQIDTNRHFHTAFELRDWAHLQTACDFLSLNGYKLI
                     WGPGRHGIGHNLFAYHRAPNGLITELFAELDQVNEELGYFEPRPWHRDHPQKPKVWPK
                     DPSASNLWGITPPDEMMK"
     gene            complement(3881..>4538)
                     /locus_tag="VM910_20880"
     CDS             complement(3881..>4538)
                     /locus_tag="VM910_20880"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HVI65015.1"
                     /translation="GPNGLIKSARPGQIIVELGSHPVPDKQRHVAAFAAKGASFLDGE
                     VSGTPGMVSARKAVIYLAGDKEACAKAERFVGGFADSCLYFGPFGAASRVKLVNNLLV
                     AINIAATAEAMALGLKAGVDVDLMIKAIATGSGGSTQFGIRAPWMAQRRFLPAQGTAP
                     ALQHYFDMIGEFAASVGVATPMLDRAVELYDKFMAMGFGEKDVAATVDVIGALPRSKA
                     "
BASE COUNT          892 a         1432 c         1462 g          752 t
ORIGIN      
        1 ggaaatcgcg gcgccgttcg gcggacgacg tcgagcggac gatccgggag cactatgtcc
       61 agggcatccg gcacttcttc atcaccgacg acaatctcgc ccgcaacaag gattgggagg
      121 cgatcttcga ccggatcatc gcgttgcgcg aacgcgagaa catgaatatt cggctgatgg
      181 tccaagtcga cacgctgtgt cacaagatcc ccaacttcat cgagaaggcc gcccgcgccg
      241 gcaccaaccg cgtgctggtc ggcctcgaaa gcatcaatgc cgcaaacctg ctcgcggcca
      301 agaagcgcca gaacaagatt accgaatacc gcacgatgct gctcgcctgg aaaaacgctg
      361 gggcgatcac caccgccggc tatatcctcg gattcccctt cgacacgccc gaatcgatcc
      421 gcgaggacat cgcgatcatc cagaaggaac ttccgctcga catcctcgaa ttcttctgcc
      481 tcaccccgct tccggggtcg gaggaccaca aggtgctgtg gcagaaaggg gagtggatgc
      541 acgaggatat gaacctctac gacggcgagc acgccgtctc gcatcatccc aagatgagca
      601 aggaggagtg ggagcaggtc tatcgctccg cctgggattg gtattacacg cccgaacaca
      661 tggaaacgat cctgcggcgc ggcgccgcca ccagctgcag catgtcgcgg ctcgtgagtt
      721 tcgtattcct gttctgctcg tcggtgccgc tcgaaaatgc ccacccgctg caaggcggtc
      781 tgctgcgccg gaagtatcgg cgggatcgcc gtcccggcat gccgatcgaa ccgatctggt
      841 cgttttatcc gaaattcgcc tgggacttcg tgagcaaata ttcccgtgcg atgcgcctgt
      901 tgctgtggct gcaccggacc aaccggcgcc tgcagaggga tccgaatcgg tacatgtaca
      961 ccgatctggc gatgacgccc gtgaccgagg acgagaccga gaagctcgag ttgttcaccc
     1021 acagcgatgc cgcgcgccaa gccgtcgcgc atgcgcataa gatcgaccag ctcacccacg
     1081 cccacacacc tgcgagggcc gaggcccacg cgtgaagcag gggcgatgcc gctaatagat
     1141 ccccggtaag atgcgcgcgg ttcgcgccat atagcggcgg taatcgtcgc caaaggtctc
     1201 gatcatcagc gcttcctcgc gtccgacgcg cagaaggaac aacgtcccga agccgatcag
     1261 gccggctggg cctgcgatcc aattcggcag caggagcgcc tgggccagcg cccacagcca
     1321 gaacgccgaa tacatcggat gccgcacgcg gctgtagacg ccgctcgtca ccagagtgtg
     1381 ctggtcgcgc acttccagcg tcaccgacca attgcgcccg agatccttgt gcgttcggtg
     1441 gaacaaccag agcgaggcgg cgaagactgc gtcgccgatc caggcttgcc aggggcgaaa
     1501 cgggtagttg gcgaagcgcg gagcgtcgct gaccacgtag ataaagggaa ggacccccaa
     1561 tcccgtggcc gaaatcgcca tcagcacgat ctcgcggccg cgatcggtgc gccgcagctt
     1621 cggcgtgcgg cgcgcgcagc ggtcgtgggg gtagcggatg atgaaccagc cgacgacgcc
     1681 gagaacccag atcaccttag cggcgagcgg cgtcatagcg aaggctgcgc cagcacggtg
     1741 cgcgggcgcg acgcatcgcg gtaagtgccg tcggctgcga cgaaatccaa cagtcccacc
     1801 ggcgagatcg tccactcgag aaatggaacc gggccgcaca ccatgagaaa atagtcgtag
     1861 ggcgcgcgcc tgtcgttggc catgaccgag cgatagtgca gccgcaagac gctgtggcgg
     1921 cagcgcgcaa acgtctcggg cagcagcata tcgtggattt gcacgcggcg gatcaccggc
     1981 ttgccgtcga gccgatcgtc gggaatgtgc cgcagcgata ccggatcgaa tttgtagaag
     2041 ctgatggtat cggcgcggga ttgatattcg gtccaggcga tcgagggctc ggccacgacg
     2101 cgcgcgatcg tgccgcggac cccctgcgcg cgcggatgca gcgcgaattt cgggatggtt
     2161 gcgccgatgg tgagcacgca aatcgtcgcg ccacgacggc cgaaatcggc gtcgcgcgca
     2221 agcgcgcgca ccacaacatc gagcacgagc atcgcgccga ggctgtggcc gacgagcacg
     2281 atttcgtcga ccgcggcctc gcgcgcacgc gcgagcagcg tctcggcaaa tcgatcgagg
     2341 cgcgcatcga tatccggacg gcgaccgtga atgtagtcgt tggcgcagat ccaatcgtcc
     2401 agcagctgct gcacgcgcca gcgccgcccg ggccagcgca gcaggacgag gaagacggca
     2461 acgccggcag ctaaaccgac ggccgcggcg cccatgcctt cgaggccgag ggacgcggtc
     2521 aacaggcgcg cggcgagcca tccgacgacc gcgaacaaga cgaccgaaag gatcggaaac
     2581 aagaaaaagc cggcgtatcg ctggttcgcg atgatgtagc gaaacatcgt gccagtgacg
     2641 atgaagtcga gatacgcgcg ggccgccttg aagagccgca gcggcagcgg tcgggtgaaa
     2701 tcgccgcgca cgatgtcgtc ccacagcagc gcttcgtgca cggtctcgac ctgccagttg
     2761 gcggcacgcg cgctgacgct ccaccaggcg cgtgattgcg cgttcgactg ctcgagcgcg
     2821 gacaaggttg cgtcgacatt ccaggtgcgt cggaaaacat cgagctggcg cgcgaaccgc
     2881 cggtattgcg cgccggcgtc gatcgggtcg tatcccgcca ggtgatagac gtgtcgacgc
     2941 cgtatcatgc tcgccgccgc ctatttcatc atctcgtccg gcggcgtgat gccccacaga
     3001 ttcgaagccg acggatcctt gggccacact ttcggtttct gcggatggtc gcggtgccat
     3061 ggccgcggct cgaaataacc gagctcctcg ttcacctgat cgagctcggc gaacagctcg
     3121 gtaatcaacc cgttgggcgc gcggtgatag gcgaagaggt tgtgaccgat gccgtgccgg
     3181 cccggccccc agatcagctt gtagccgttg aggctgagaa aatcgcaggc ggtctgcagg
     3241 tgcgcccagt cgcgcagctc gaacgccgtg tgaaaatgcc ggttcgtgtc gatttgcatc
     3301 aggttgatgg tgtggtgatc gcggccgcag cgcaggaacg agaagaaatc cgccatccaa
     3361 tcggactcgc gaaagccgag cacgtcacaa tagaacttgg tcacgtgctt gacgtcggtg
     3421 acgaaaaaag cgacgtggcc gagcttgtgc ggaacgattc ccttggtcgg aaagcgctgg
     3481 cccgagaacg cgtcccgctt gaacacctcc atcaccgttc ccttcgggtc ttcgaatgtg
     3541 accatatccg agatcgaggg ttcgggatcc ttgcggcgct gggttttgat gccatggccc
     3601 tgaacgtgct tctcgaacgc gccgagatcg tcctcggggg cgatctggaa gccgaggcgc
     3661 acgcatttcg cctgcgatcc cttgcgcaat accaccgagt gatggtcgac ggtcgaggcc
     3721 agatagacgg catccttgcc cttgtcggtc accgtgaggc cgagcacctc ggtgtagtat
     3781 tcggtctgct tgtccacgtc gggcatttcg taaacggcgt gggcgatctt cttgacgcgg
     3841 atcatgtggt cctccgtgat tgcttgtttc ccgtcgaaag tcaggccttg gagcgcggca
     3901 gcgcgccgat gacgtcgacc gtcgcggcga catccttctc gccgaaaccc atggccatga
     3961 acttgtcgta gagctcgacc gcgcggtcga gcatgggcgt ggcaacgccc acgctggcgg
     4021 cgaactcgcc gatcatgtcg aagtagtgct gcaatgcggg cgccgtgccc tgcgcgggaa
     4081 ggaagcgccg ctgcgccatc caaggggcgc ggatgccgaa ctgggtcgaa ccgccgctgc
     4141 cggtggcgat ggccttgatc atcagatcga cgtcgacgcc ggccttcagg cccagcgcca
     4201 tcgcctccgc ggtcgcggca atgttgatgg ccacgagcag attgttgacg agtttcacgc
     4261 ggctggcggc gccgaacggc ccgaaataga ggcaggaatc ggcaaatccg ccgacgaagc
     4321 gctcagcctt ggcgcaggcc tccttgtcgc cggcgagata gatcacggcc ttgcgcgccg
     4381 ataccattcc cggcgttccg ctcacctcgc cgtcgagaaa gctcgccccc ttggccgcaa
     4441 acgcagccac gtgacgctgt ttgtcgggga cgggatggga gccaagctcg acgatgatct
     4501 gtcccggccg ggcggatttg atcagcccat tcgggccc
//
LOCUS       DATLJT010000907         1834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1438440,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000907 DATLJT010000000
VERSION     DATLJT010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438440"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VM910_20885"
     CDS             <1..781
                     /locus_tag="VM910_20885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65016.1"
                     /translation="RGGAGYSIAAQSVMRAEHDGYTLLASETGFYTTQPHLYAEGKLA
                     YDAEKDFVPVAGYGTIPTALLVNPSVPVNSVRDLIALAKTKPAAVTFGTAGIGTALHI
                     AGLELENLASIKMTAVHYRGAAPALTDVIAGHVDAVIMGPSVALSSVKDGKLKMLAFG
                     SEKRLPQFPDVPTIAETVPGYEASVSFGLFAAAGTPADIVKKVNADVQKIVSDPEYQK
                     KYLERFAVQPVPGPLDAFAEYLRKDSAKWGEVIKAANVKIE"
     gene            1102..1686
                     /locus_tag="VM910_20890"
     CDS             1102..1686
                     /locus_tag="VM910_20890"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HVI65017.1"
                     /translation="MSEATSHGHAPPFNAAPLRTGLRARIAWICQALRLAAVIWVVWE
                     LVFVVIHWSDKAAVLTSYERVFSTVLVVSTARYAVAFGVVMVSLAVIFVIAFCLWRLA
                     GTYLAGRVFTVDAALWLRRTAIAGLAGIIVSVIARVVVASILTGRWVPMSPLGFFYIV
                     PQDVLHLIFVIFVLALAHIFKAAAEMADDQAQIV"
     gene            1695..>1834
                     /locus_tag="VM910_20895"
     CDS             1695..>1834
                     /locus_tag="VM910_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI65018.1"
                     /translation="MPIIVNLDVMLARRKTRSKELAEQIGISEQNVSLLKSGKVKGVR
                     FET"
BASE COUNT          336 a          540 c          569 g          389 t
ORIGIN      
        1 ccggggcggt gccggatatt cgatcgcggc gcagtccgtg atgcgggccg agcacgacgg
       61 ttacacgctg ctcgcctcgg agaccggctt ctacacgacc caaccgcatc tctacgctga
      121 agggaagctg gcctacgatg cggagaagga cttcgttccg gtcgccggct acggcaccat
      181 tccgacggcg ctgctagtga atccgtcggt gccggtgaat tcggtgcgcg acctgatcgc
      241 gctcgccaag acgaagcccg ccgccgtgac tttcggcacc gccggcatcg gcactgcgct
      301 gcacatcgcc ggactggagt tggagaatct cgccagtatc aagatgacgg cggtgcacta
      361 ccgcggcgcg gcacctgcac tgactgatgt gattgctggg cacgtcgacg cggtgattat
      421 gggcccatcc gtggcgttga gttcagtgaa ggacggaaaa ctcaagatgc tcgctttcgg
      481 cagcgagaag cgcctgccgc agtttcccga cgtgccgacc atcgccgaga ccgttccggg
      541 atacgaggcg agcgtatcgt tcgggctgtt tgccgcggca ggcacgcccg cggacatcgt
      601 caagaaggtc aacgccgacg tgcaaaagat cgtcagcgat cccgaatacc agaaaaaata
      661 tctcgaacgc tttgcggtgc agccggttcc tggcccgctc gacgcctttg ccgaatattt
      721 acgcaaggac tcggccaaat ggggcgaggt catcaaggcg gcgaacgtga agatcgaatg
      781 agtgccgctg tgccagcttg ggtaggcaag gcaccagctt cgttatgagc gaagttgccg
      841 ggtcgtttga ctgccacgaa ttcagctctg aatggtgaac catcgagcac ttcgactcgg
      901 cgtcctttgc ctgctgcgtc ctcgacgaac atcatctcgc ctcggccgcc gtcgaccgcg
      961 atgccgagga cgcagagctg ttggttaatt cggaatcgga gcggtccggg tcataaatat
     1021 gacgcattta cttattatcg ttatgcgata atatatctac gcactaattg aacttattcg
     1081 cagcgtcagg ggagagacac gatgtcggaa gctacgagcc acggccacgc gccaccattc
     1141 aacgcggcgc ctctgcggac cggcttgcgc gcgcgcattg cttggatttg ccaagcgctg
     1201 cgccttgcgg cggtgatttg ggtcgtctgg gaattggtct ttgtcgtcat ccattggagc
     1261 gacaaggcag cggtgctgac gagctatgaa cgggtgttct cgactgttct cgttgtatcg
     1321 acggcgcgct acgcggttgc ctttggtgtg gtcatggtca gcctggccgt catcttcgtt
     1381 atcgccttct gcctttggcg gctcgccggg acctatctgg ccgggcgcgt atttaccgtc
     1441 gatgccgcgc tgtggcttcg ccgcacggcc attgcgggtc tcgccggaat catcgtcagc
     1501 gtgatcgctc gcgttgtggt cgcgagcatt ctgacgggtc gatgggtgcc gatgtcgccg
     1561 ctcggcttct tctacatcgt gccgcaggat gtccttcacc tgatcttcgt cattttcgtc
     1621 ttagcgctcg cccacatctt caaggcggcg gccgagatgg ccgatgatca ggcgcagatc
     1681 gtctaggaga cctcatgccc atcatcgtca acctcgatgt catgctggcg cgccgcaaga
     1741 cgcgctcaaa ggagctggcc gagcaaatcg gcatcagcga gcagaacgta tcgctgttga
     1801 agtcgggcaa ggtcaaaggc gtccgcttcg agac
//
LOCUS       DATLJT010000908         4044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2127144,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000908 DATLJT010000000
VERSION     DATLJT010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127144"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1620
                     /locus_tag="VM910_20900"
     CDS             100..1620
                     /locus_tag="VM910_20900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65019.1"
                     /translation="MSSAAQYASRLGPRESAEGGTITTDIPARLDRLPWSRFHLLVMV
                     ALGVTWILDGLEVTIIGAIAPVLQNTQTLGLSAGEIGAAGSAYVIGAVVGALLFSWLT
                     DRFGRRLVFYVTLIVYLLGVLLTATSWSFWSFALFRAVTGLGIGGEYAAINSAIDELI
                     PARFRGRVDLIINGSFWLGAAAGAGASLLFLDPNLIAPNLGWRLGFAIGGTLGLGILL
                     LRHYVPESPRWLITHGWTRKADATIADIEQRVRSDTGAELEPPHDRLRVHPRRSFGFG
                     LIARAMFGKYRSRSLVVLALMVAQAFLFNAVFFSYGLVLSKFHGVPEEGTGLYLMTLA
                     VSNFLGPLLLGPLFDTIGRRAMIAGTYAAGGVLLIATAIVFGLDAFTAWTQTFAWMLI
                     FFFASAAASSAYLTASEVFPLETRARDRRLLRRRHGNRRYFGARAVRPSHRNRLALGA
                     CRGIHLRRIADACCRRCRGRIRDRRGNALARKHRWSALERIASSPPRSQHHTSRRL"
     gene            complement(2619..2912)
                     /locus_tag="VM910_20905"
     CDS             complement(2619..2912)
                     /locus_tag="VM910_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65020.1"
                     /translation="MSTSAPFEYRTEALLENPNSRLVIVGQILCILVPLAVWFSPLPL
                     EPVTKHGFAIVGFMVVAWITRAMDYALAGLVGCFLFWALGVVRFSVAFSGFDR"
     gene            complement(2937..3179)
                     /locus_tag="VM910_20910"
     CDS             complement(2937..3179)
                     /locus_tag="VM910_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65021.1"
                     /translation="MSPNKEKLALFIDGANLYATAKIARLRRKFMTLLCGAVAWPLAA
                     RAQQPMPMIGFLGAGSLDNYGLYLAFRLGTPMGVTR"
     gene            complement(3716..>4044)
                     /locus_tag="VM910_20915"
     CDS             complement(3716..>4044)
                     /locus_tag="VM910_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI65022.1"
                     /translation="IHVPAHNTPIKASRREALLIAIAKARQWIDDLAHGRAASFAVIA
                     RREGKAERHIRLLAPLAFVSPRIVSALLDDTAPADLTLTKLARALPYCWAEQERRVGT
                     SAPFHG"
BASE COUNT          707 a         1290 c         1269 g          778 t
ORIGIN      
        1 cgcctccgca gcaagcgtac tcgcagcaaa tttccaatct cgcggaactg ccgctggctt
       61 gctccaagtt gcaggaccag accaacgacg ggaatttgca tgtcctctgc cgctcaatac
      121 gcgtcgcgct tgggccctcg cgagagcgca gagggcggca ccattacgac ggatattccc
      181 gctcggctcg atcggcttcc ttggagtcgt ttccacctgc tggtgatggt cgcgctcggg
      241 gtaacctgga ttctcgatgg gcttgaagtc accatcatcg gggcaatcgc ccccgtgctc
      301 cagaacacgc aaacgctggg gctctctgcc ggggagatcg gtgcggccgg ctcggcgtat
      361 gtgatcggcg cggttgtggg cgcgctgctg ttcagctggc tcaccgaccg gttcggccgg
      421 cggctcgttt tctacgtcac gttgatcgtc tatctgctcg gcgtcctgtt gaccgcgacc
      481 tcctggagct tctggagctt cgcactgttc cgcgcggtca ccgggctcgg catcggcggc
      541 gaatacgcgg ctatcaactc ggcgatcgac gaactcattc cggcgcgatt ccgcggtcgc
      601 gtcgatctca tcatcaacgg cagtttctgg ctgggcgcgg cggctggagc cggagcgtcg
      661 ctgctgtttc tcgatccgaa tttgatcgcg cccaacctcg gatggcggct cggcttcgcc
      721 atcggcggaa ccctcggact cggcatcctg ctgctgcgcc attacgtacc ggaaagcccg
      781 cgctggctta tcacccatgg ctggacccgc aaagccgatg cgaccatcgc cgacatcgag
      841 cagcgcgtgc gctcggacac cggcgccgag ctcgagccgc cgcatgaccg gctgcgcgta
      901 cacccgcgca ggagcttcgg gttcgggctt atcgcacgcg caatgtttgg caagtatcgc
      961 agccgcagtc tggttgtgct tgccttgatg gtggcgcaag ccttcttgtt caacgcggtg
     1021 ttcttctctt atggcctcgt cttgagcaaa ttccacggcg ttcccgagga agggaccggc
     1081 ctctacctca tgacgctggc ggtgagcaat tttttgggcc cgctgctgct cggtccgctg
     1141 ttcgacacga tcggccggcg tgccatgatc gccggcacct acgccgccgg cggcgtcctg
     1201 ctgattgcga cggcgatcgt cttcggcctt gatgccttca ccgcctggac gcagacattt
     1261 gcgtggatgc tgatcttttt cttcgcgtcg gcggctgcga gctccgccta tctgaccgcc
     1321 agcgaggtgt ttccgctgga gacgcgcgct cgcgatcgcc gtcttctacg ccgtcggcac
     1381 ggcaatcggc ggtactttgg cgcccgtgct gttcggccat ctcatcgaaa ccggctcgcc
     1441 ctgggcgctt gccgggggat acatcttcgc cgcattgctg atgcttgctg ccgccgttgc
     1501 cgaggccgga ttcgggatcg acgcggaaat gcgctcgctc gaaagcatcg ctggtccgct
     1561 ctcgagcgaa tagcttcttc cccgccgcgc agtcagcatc acacctcgcg ccggctatga
     1621 gctcgcggcg gcgtttggcg agataggcgt cgcgcgcgcg gcttgacgga agcttggcgg
     1681 cgtaactagc tgtctcggtg gcccaggcga cgatttttct cgtcagttcc ggtcatcagc
     1741 gcctcgatcg cgcgcgcatc ctcggcgggc tgatcgcctt cggcattcga tgtagcttcg
     1801 gagcctcatg cgaacgagcg cgtccgtgct ttcgctgaac gtgctggaag tcgggaccgc
     1861 tatgatcacc gacgtacgca tagaccagtt gctgccgtgc caggggtcgc gcacttcgct
     1921 ccgtcggtcg caccgctcgc gcaaacaaaa ccagccagga tttcgtcgtc tccgcagcga
     1981 acgctatccg tacccgtgac gacgcgaatt gccgatgccg ggccggggtc acccttcggg
     2041 ccagctggtc cggcggggcc gggtggacca agggggcctt gtggccctgg tggtccgcgc
     2101 tcgccggcgg gtccgactgg gccttggcca cccgcttctc cctgcggccc ctgcgggcca
     2161 actggtcctg gcggtcccga agggccagcc ggcccctgct caccgactga gccctgggga
     2221 ccgacgggtc cttgctggcc ttgagcgccc tgggggccgg ccggaccggt tggtccggca
     2281 gggccagcct caccagcagg ccctgaagga ccttgcggtc ccgtgcaagc cgccaacgcg
     2341 atcatcgcga tggcagcagg gatggtcacc acctgtcgca tcagcgctcc cccggggaga
     2401 caaggacact gcgctcgcat gtcgactgcg gtcaagcagc gacaatcgtg ggtaagtcgt
     2461 gaagcacctt gcgcaaacca gcacgaacac cttccgcctg cgcccgcgcg gctacgatgt
     2521 aagagcatgt cgtcgccacc gccgagcact gctcatgtgc tcgaacgcac cagcgtcaag
     2581 cttgccgtcc atgaacagcg cgaggtggac ttgacgggtc aacggtcgaa cccgctgaac
     2641 gccaccgaaa atcggacgac gccgagcgcc cagaacagaa agcagcccac gagccccgca
     2701 agcgcataat ccatggcccg cgtaatccac gccaccacca tgaagccgac gatcgcgaag
     2761 ccatgtttgg tcactggctc gagcggcagc ggcgagaacc atacggccag cggaacaagg
     2821 atgcaaagaa tttgcccgac aatgacaaga cgagagttcg gattttcaag caacgcctca
     2881 gtgcggtatt caaagggtgc ggacgtgctc acggatgatt tcgcgccggc cgacctttat
     2941 cgggtgaccc ccatcggagt gcccaagcgg aaagccagat aaagtccgta gttgtcgagc
     3001 gaaccggcgc ctagaaatcc gatcatcggc atcggctgtt gcgcgcgcgc cgcgagcggc
     3061 caggccaccg cgccgcaaag cagcgtcatg aacttgcgtc gaagccgagc gattttcgcg
     3121 gtggcataga gattggcacc gtcaatgaac agcgcgagtt tttctttgtt gggagacaaa
     3181 agcctgatta ccgacgagcg agcatcccgc gcacgactgc gctggggcag tcaggacgaa
     3241 atcccgcgtt tcggttctgc ccttaatcca tggggtgttc catgcgacct cgtcgcttcc
     3301 ggcggaattg atcgttggcc gttcgcgcga cattctttac cccgatgacg tgcagacccg
     3361 acgcggaata cagagaccgg actgcgtggc tgacgatgca gtcagctgcg aactagtctc
     3421 cgcaccaaat tccctgctaa cagggaaatt aacagggaat ttcgcaattc cgggcctcag
     3481 caagcgattt tcgcatctaa tcaacgagct aattcaaagg cttgcagcca aattccctac
     3541 gcaacggaac agggaatttt tgcagccata acagggaatt ttttcgggat aacagggaat
     3601 ttaatcgagc gaacagggaa agcgctgagc tgctgtcggg acgtcgaatt agcacggctc
     3661 ttgatgtcaa ctcttggcag cgggtgcagc ggacatcgcc ggcgatgcgc cagaattagc
     3721 cgtggaacgg cgccgaagtt cctactcgcc gttcctgctc agcccagcaa tagggcagcg
     3781 cgcgggcgag ctttgtgagg gtgagatcgg ccggcgcggt atcatcgagc agtgccgaca
     3841 cgatccgggg tgagacgaag gcgagcggtg ccaacagccg gatgtgccgc tcggcctttc
     3901 cctcccgccg ggcgatgacg gcgaaactgg cggcgcggcc gtgtgccaaa tcgtcgatcc
     3961 attggcgggc cttggcgatc gcgatcagga gcgcctcgcg gcggctagct ttgatcggcg
     4021 tgttgtgcgc gggcacatgg atga
//
LOCUS       DATLJT010000909         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3276575,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000909 DATLJT010000000
VERSION     DATLJT010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3276575"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..327)
                     /locus_tag="VM910_20920"
     CDS             complement(7..327)
                     /locus_tag="VM910_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI65023.1"
                     /translation="MAKITFVDHRGESRTIEVENGATVMEAAIRNAVPGIEAECGGAC
                     ACATCHVYVDEAWREKVGSPSPMEEDMLDFGFDVRPNSRLSCQIKVSDELDGLVVSTP
                     ERQA"
     gene            complement(480..857)
                     /locus_tag="VM910_20925"
     CDS             complement(480..857)
                     /locus_tag="VM910_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVI65024.1"
                     /translation="MPTHVQRIDWMPSPPLVPDENPLDPQHLQRMTLGDAALEREVLA
                     MFVHQAARLVNALATLPADAPAMAHTLKGSARAIGASRVAEHASALELAIGDGRDPRS
                     ILAELNDAVAEARRAIDALLARP"
     gene            1247..>2679
                     /locus_tag="VM910_20930"
     CDS             1247..>2679
                     /locus_tag="VM910_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="negative regulator of septation ring formation"
                     /protein_id="HVI65025.1"
                     /translation="MANNPKKVKDPTEVALSAIQEALNISDANNDANRGGARGEVAPP
                     IPPAPPTFDEPTFDARLGAERSMFEQMEEPRRRAANDDRETIGQILQAIQKGRPARNI
                     YTLATLFAGVWVVGCGLLTVSFLPSLQAAMGQGAGGVFVLAGLAALFFAPVLLFYFLA
                     SLAWRGQELRMIAQSMAHVAIRFSDPEGAASDSVVTVGQAIRREVAAMGDGVERAIAR
                     AGELETLVTNEVAALERAYTDNEVRIRALLQDIAHQRDNLVGQAEQVRSAISGVQIDL
                     RHDIALISDAIASRVDEVAKSITSALEERGAHITQALSNAGDNMILALGERGGDLLDR
                     LEEASSETTRAVLDASERLTTSLNFKTGHVHDEFVDLADRVHELLNERIDRITGEFEQ
                     RSSSIVDGISERTEQVHDSLKNSGDSLLLELELRSNDLVSKIDEAGSRLATHILSSGD
                     KASDALDVTVNSLVAKVVGQTETAHDTL"
BASE COUNT          503 a          845 c          849 g          482 t
ORIGIN      
        1 tgcgcctcaa gcctgccgtt cgggcgtcga aaccacgaga ccgtcgagtt cgtcgctcac
       61 cttgatttgg caggacaggc gcgagttcgg ccgcacgtca aagccgaaat cgagcatgtc
      121 ctcctccatc ggcgaaggcg aacccacttt ctcgcgccag gcctcgtcga catagacatg
      181 gcaggtcgcg caggcgcagg cgccgccgca ttcggcctcg atgccgggaa cggcattgcg
      241 gattgccgct tccatgacgg tcgcgccgtt ttcgacttcg atggtgcggg attcgccgcg
      301 atggtcgaca aaggtgattt tggccatgtt cgctcgtatt gcggggaaaa acaggttccg
      361 ggcagtccta taacgggtcg gacagatcaa ggctagggat gcaaatgcgg gaacggcgct
      421 ccggcggctc cgggaaggcg gcgaccatcg gcgattccga agcggtattt ttgacggtgt
      481 cagggacggg cgagcagcgc gtcgatcgcc ctgcgcgctt ctgccacagc gtcgttgagt
      541 tcggcaagga tggagcgtgg atctctgcca tccccgatcg caagctccag tgcgctggca
      601 tgttcggcca cgcgggaggc gccgatcgca cgcgccgaac ccttaagcgt gtgtgccatt
      661 gccggggcat cggcaggcag cgtcgcgagc gcgttgacca ggcgcgcggc ctgatgcacg
      721 aacatcgcca gcacttcgcg ctcaagcgcc gcatcgccga gcgtcatgcg ctgcagatgt
      781 tgcgggtcga gcggattctc atcgggaaca agcggcgggg acggcatcca gtcgatccgt
      841 tgcacgtggg ttggcatgcg ggagtgtccc gattttcgaa gctcgctccg ggcgaagatc
      901 agccaagcca accatgaaac tcgttaaggg aacgtttggt gtaccggccg catcttcgcc
      961 ttgattttga ccgcgagccg ccgcattcat ccgggtttcc gcaaaaaacc tgtgtacgat
     1021 tagtatctta cggcgttctt cggttaacga tgattaagat tgtattaatc gcgccatttc
     1081 aaaagcagca ctcaacaaat aggatgctgc ccaagtggag gggggcgcgg cgggttgccg
     1141 tgctttgacg ctgcttgaat cacgatgccg acttcgacct tgcgcgaggg caactcgcac
     1201 gaggatcgtt gcgcgatgcg tacaagtatg aggggctcag gctgtcatgg cgaacaatcc
     1261 caaaaaggtc aaagacccta cagaagtcgc gctgtctgcg attcaggaag cactgaacat
     1321 cagcgacgcc aataacgatg cgaaccgtgg tggagcacgc ggcgaagtcg ccccaccgat
     1381 cccgcctgca ccaccgacct tcgacgagcc gaccttcgac gcgcgtctgg gcgccgagcg
     1441 ttcgatgttc gagcagatgg aggagccgcg ccgccgcgcg gcaaatgacg accgcgagac
     1501 catcggtcag atcctgcagg cgattcagaa gggccgccct gcccgcaaca tctacacgct
     1561 cgccaccctg ttcgccggtg tctgggtcgt cggttgcggc cttctgaccg tcagcttcct
     1621 cccctcgctg caggctgcga tgggccaggg tgccggcggc gtgttcgtgc tcgcgggcct
     1681 tgcggcgctg ttttttgctc cggtgctgct gttctatttc cttgcgagcc ttgcctggcg
     1741 cggtcaggaa ttgcggatga tcgcgcagtc gatggcgcac gtcgccattc gcttctccga
     1801 tccggagggc gcggcatccg actccgtggt gacggtcggt caggcgatcc gccgcgaggt
     1861 cgcagcgatg ggcgatggcg tcgagcgcgc gatcgcgcga gccggcgaac tggaaaccct
     1921 cgtcaccaac gaagtcgccg cgctggagcg cgcctatacc gacaacgagg tgcgcatccg
     1981 cgcactgttg caggacatcg ctcaccagcg cgacaatctg gtcggacagg ccgagcaggt
     2041 ccgcagcgcc atttccggcg tgcagatcga cctgcgccac gatatcgcgc tgatctccga
     2101 tgcgatcgcc tcgcgcgtcg acgaggtcgc gaaaagcatc accagcgcgc tggaggagcg
     2161 cggcgcccac atcacgcagg cgctgagcaa tgccggcgac aacatgatcc tggcgctcgg
     2221 ggaacgcggc ggcgacctgc tggaccgcct ggaagaagcg agttccgaaa ccacgcgcgc
     2281 cgttctcgat gcctccgagc ggctgaccac cagcctcaat ttcaagaccg gtcacgtcca
     2341 cgacgaattc gtcgacctcg ccgatcgcgt ccacgaattg ctgaacgagc ggatcgaccg
     2401 catcaccggc gaattcgagc aacgctcctc gtcgatcgtc gacggcatct ccgaacgcac
     2461 cgagcaggtc catgactccc tgaagaactc gggcgattcg ctgttgctcg agctcgagct
     2521 gcgcagcaac gatctcgtca gcaaaatcga cgaggccggc agccggcttg caacccatat
     2581 cctctcctcc ggcgacaaag cgagcgacgc gctcgacgtc accgtcaatt cgctggtggc
     2641 gaaggtggtc ggtcagaccg agactgcgca cgacacgct
//
LOCUS       DATLJT010000910         1773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_60083,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000910 DATLJT010000000
VERSION     DATLJT010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60083"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VM910_20935"
     CDS             complement(<1..736)
                     /locus_tag="VM910_20935"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVI65026.1"
                     /translation="MLAAFLLGPASKSAAAEDGFFEQDKLTGDWGGARKTLKDAGIDL
                     GLNYVAETLSNPVGGFKQTTIYQGLLTPSLNLDLEKLANWPGATFYTDAFQISGRGLS
                     RNAIGNLLAVSTIEALPSTRLHDLWLQQEALDRQVSLRVGQIAIDDEFYISQYSANFI
                     NSTFGCPDILSTDLPSGGPCYPFAVPGVRLRGAPTAGLTLSAAVFNGNPAPPGPRDPQ
                     VRNSSGTNFLIGEGGSLALAELAYSFD"
     gene            complement(818..1267)
                     /locus_tag="VM910_20940"
     CDS             complement(818..1267)
                     /locus_tag="VM910_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65027.1"
                     /translation="MASSTLEVEARDALPGFRRTDLPRYLALHMARAGLSEWRFEPTT
                     GREVAANRVEWSFKLNPYAGGEVRSFGHTLTQDWGFGVHRPITIEARLYLNGEYQTLV
                     EQQALVQGGPDDLDLAAAVASATRNLLGPSGAYRAIDTGLGRANHAR"
     gene            complement(1290..1484)
                     /locus_tag="VM910_20945"
     CDS             complement(1290..1484)
                     /locus_tag="VM910_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65028.1"
                     /translation="MTETLGLAAEENSRVLELASLFGYRAVIRLSVEHAPRQENTLLA
                     RDYGAPPHRLARVLGVGGGG"
     gene            complement(1477..>1773)
                     /locus_tag="VM910_20950"
     CDS             complement(1477..>1773)
                     /locus_tag="VM910_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HVI65029.1"
                     /translation="FISSADWMPRNLDGRVETLVPIENPTVHQQILDQIMEANLHDDA
                     QSWILRPDGSWERAVPRAKPQSAHVYFMTNPSLSGRGSALHGHNAHSHRLRVHD"
BASE COUNT          362 a          549 c          545 g          317 t
ORIGIN      
        1 tgtcgaacga gtaggcaagt tccgcgagcg cgagcgagcc gccctcgcca atcagaaagt
       61 tggtgccgct cgagttgcga acctgtggat ccctcggccc gggtggtgct ggatttccgt
      121 tgaagacagc ggcggataag gtcaaacccg cggtcggcgc gccacgcagc cgcacaccag
      181 gtaccgcaaa aggatagcac ggcccgccgc tcggcaggtc ggtggacaag atgtccggac
      241 agccgaacgt cgagttgatg aagttcgccg agtattgact gatataaaac tcgtcatcga
      301 tagcgatctg gccgacgcgc agagatacct ggcgatcgag cgcctcctgc tgtagccata
      361 gatcgtgcag gcgcgtcgag ggtagcgcct ctatcgtgct gaccgccagt aggttaccga
      421 tggcgttccg cgataagcca cggccgctga tctggaaggc atcggtgtag aaggtcgcgc
      481 caggccaatt tgcgagcttt tcgagatcga gattgagaga aggcgtcagc agcccctggt
      541 aaatcgttgt ctgcttgaag ccaccaacag ggttggagag cgtttccgcg acgtaattga
      601 ggccaagatc gatgccggcg tctttcaacg tcttgcgggc cccaccccag tcgccggtca
      661 acttatcctg ttcgaaaaac ccgtcctccg ccgcggcaga tttgctggcc ggtcctagta
      721 aaaacgcagc gagcaacaca agcgcgggaa ggcggctacc tcgcacgagc cttagaccgg
      781 cagccctgcg ggcggcaagt cagttgaaaa gcaccggcta tcttgcgtgg ttggctcgac
      841 caagcccggt atcgatggcg cggtaggctc cagacggccc gaggagattt cgtgtggcgc
      901 tcgcgaccgc cgcggcgaga tcaagatcgt cgggcccccc ttggaccagc gcctgttgtt
      961 cgacgagggt ctgatactcc ccattgagat agagccgcgc ctcgatggtg atcggacggt
     1021 ggacgccgaa cccccaatcc tgggtcagcg tgtgcccaaa actgcgtact tcccctccgg
     1081 catatgggtt caatttgaag ctccactcga cgcgattagc cgcgacctca cggccggtcg
     1141 tcggctcaaa ccgccattcg gagaggccgg cccttgccat gtggagtgcc agatagcgcg
     1201 gcaggtcggt gcggcgaaaa cccggcagcg catcgcgcgc ttcaacctcc aacgtgctcg
     1261 acgccatggc agaaaccaag gtaaacggct cagccaccgc cgccaacacc cagcacacga
     1321 gcaaggcgat gcggtggcgc gccataatca cgggcgagaa gcgtgttttc ctgtcgcggc
     1381 gcatgctcaa ccgacagccg gatcaccgca cgatagccaa ataatgaagc aagctccagc
     1441 accctgctgt tctcctcagc ggcgagaccg agcgtctcag tcatggaccc tcagccggtg
     1501 cgaatgcgca ttgtgtccat gcaatgcact cccgcgaccc gagaggctcg ggttcgtcat
     1561 gaaatagacg tgcgcgctct gaggcttggc ccgcggaacc gcccgttccc atgagccgtc
     1621 agggcgcaag atccagctct gcgcgtcgtc gtggaggttc gcctccatga tctggtcgag
     1681 gatctgctgg tgcacggtcg ggttctcgat cggaaccaat gtttcgacgc gaccgtccag
     1741 atttcgcggc atccaatccg ccgacgaaat gaa
//
LOCUS       DATLJT010000911         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_290601,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000911 DATLJT010000000
VERSION     DATLJT010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290601"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /gene="ugpB"
                     /locus_tag="VM910_20955"
     CDS             complement(<1..1241)
                     /gene="ugpB"
                     /locus_tag="VM910_20955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HVI65030.1"
                     /translation="MALRRLGAAAAVAATLVFAASPAHAVVEIQWWHAMTGGNNDVVV
                     KLANDFNASQSDYKVVPTYKGGYADTMNAGIAAFRAGNAPHIMQVFEVGTATMMAATG
                     AIKPVYKLMQETGEKFDPGNYLPAITGYYSTSKGEMLSFPFNSSSTVMWVNLDALKKA
                     DIAAIPKTWPELFDDAKKLKAAGYDKCGFSNAWVTWVHLEQLSAWHNVPLSTKANGLD
                     GFDTVLEFNGPLQVKHLEKLIELQKDKTYDYSGRTNAGEGRFTSGECPIFLTSSAFFG
                     NVKSQAKFNFTNAPMPYYPDVAGAPQNSIIGGASLWVMGGKTAEEYKGVAKFLAFLSD
                     TDRQVEIHKASGYLPITKAAYEKAKAQGFYKEQPYLETPLMELTNKPPTENSRGLRLG
                     NMVQLRDIWAEEIEQALAGKKT"
     gene            complement(1382..2593)
                     /locus_tag="VM910_20960"
     CDS             complement(1382..2593)
                     /locus_tag="VM910_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI65031.1"
                     /translation="MTTEAAPAPGAMTGLRVIDLSRVLGGPYCTQILADHGADVIKVE
                     PPAGDETRDWGPPFHEEDAAYFVGVNRNKRSIGLDLASAGGRAVLMKLLETADVLIEN
                     FKPGTLDKWGIGNDVLRQNFPRLVHCRISGFGGDGPRGGNPGYDAIIQAMTGMIAATG
                     SKESGPMRIGVPLVDITTGLYAAIGILMALSERQKSGLGQFLETTLYETGLAIMHPHT
                     ANYFMHGKPPSLTGNEHPNLVPYAIFPTKTDDIFIGVGNDGTFRKLAKEIGKEELGSD
                     PRFARNKDRIANREALRAELAAVFSQHEAEPLCNRLLAAGLPAGPVRKVDQALTNEHT
                     VYRGDVIARDWYKGVASPIRMQRSKPSLRRVPPKFSQHTIEVLGEFGYSRSEVDAMIA
                     SGAVCGSARRR"
     gene            complement(2754..3179)
                     /locus_tag="VM910_20965"
     CDS             complement(2754..3179)
                     /locus_tag="VM910_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HVI65032.1"
                     /translation="MPVNVLYKTSAKATGGRDGQAGTLDGTLNVKLATPKELGGAGGA
                     GNNPEQLFAAGYAACFIGAMKFVASQGGPKVPADASVTSIVGIGPRSEGGFGLDIELG
                     ISLPGLARAEAEALVAKAHQVCPYSNATRGNVDVRLKIV"
     gene            3336..>3692
                     /locus_tag="VM910_20970"
     CDS             3336..>3692
                     /locus_tag="VM910_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI65033.1"
                     /translation="MARKHASEVPLQLGSQLCFAIYSAAHAFNRVYKPLLDRLGLTYP
                     QYLVMLVLWERDGVPVKDIGERLFLDSGTLTPLLKRLEAAHLIKRARSAEDERQVLIS
                     LTSQGHALKEKARALPQ"
BASE COUNT          646 a         1137 c         1172 g          737 t
ORIGIN      
        1 gtcttcttgc cggcgagcgc ctgctcgatc tcctccgccc agatgtcgcg aagctgcacc
       61 atgttaccga gccgcaagcc ccgcgaattc tcggtcggcg gtttgttggt caattccatc
      121 agcggcgttt cgaggtaagg ctgttccttg taaaagccct gcgccttggc cttctcgtag
      181 gccgccttgg tgatcggcag gtagccggag gccttgtgga tctccacctg acggtcggtg
      241 tcggacagga aggcgaggaa cttggccacg cccttgtact cctcggccgt cttgccgccc
      301 atcacccaca gcgatgcacc gccgatgatc gaattctgcg gcgcgcccgc gacgtccggg
      361 tagtagggca tcggcgcgtt ggtgaaattg aacttggcct gcgatttcac attgccgaag
      421 aaggccgacg acgtcaggaa gatcgggcat tcgcccgagg tgaagcggcc ctcaccggca
      481 ttggtacgcc ccgaatagtc gtaggtcttg tccttctgca gctcgatcag cttttccaga
      541 tgcttgacct gcagcggccc gttgaattcg agtacggtgt cgaagccgtc gagcccgttt
      601 gccttcgtcg acagcggcac gttgtgccag gccgaaagct gttcgagatg gacccaggtg
      661 acccaggcgt tggagaagcc gcatttgtcg tatccggcag ccttcagttt tttggcgtcg
      721 tcgaacagct ccggccaggt cttcgggatg gcagctatat cggccttctt caacgcatcc
      781 agattgaccc acatgaccgt tgaggacgag ttgaagggga acgacagcat ttcgcccttc
      841 gaggtcgagt agtagccggt gatcgccggt agatagttgc ccggatcgaa cttttcgccg
      901 gtttcctgca tcagcttgta gaccggcttg atggctccgg ttgccgccat catggtcgcg
      961 gtgccgacct cgaacacctg catgatgtgg ggcgcgttgc cggcgcggaa tgcggcgatg
     1021 ccggcgttca tggtgtcggc gtagccgcct ttataggtcg gcacgacctt gtagtcgctt
     1081 tgcgaggcgt tgaaatcgtt ggcgagcttg accacgacgt cgttgttgcc gccagtcatg
     1141 gcgtgccacc actggatctc gaccactgcg tgcgccggtg acgcagcaaa gaccagcgtc
     1201 gccgcgaccg ccgcggccgc gcctaatcgt cgtaaagcca tcaaacccct cccgttggat
     1261 cgccgtcgcc ttgattagcg ccgtaacagc gcccggtgac gcgcgaatga ccgtagcagc
     1321 cgatgatggt acgggggaag ccgagccgaa gggaagttaa aaaatgggag caggagccgc
     1381 cctaacgcct ccgcgcggag ccgcagaccg caccgcttgc aatcatcgca tcgacttcgc
     1441 tcctcgaata gccgaactcg ccgagcacct cgatcgtgtg ctggctgaac ttcggcggca
     1501 cgcgcctcaa gctcggcttg ctgcgttgca tgcggatcgg cgaggcgacg cccttgtacc
     1561 aatccctggc gatgacgtcg ccccgataga cggtgtgctc gttggtcagc gcctggtcga
     1621 ccttccgcac gggaccggcc ggcaggccag cggcaagcag acgattgcag agcggctcgg
     1681 cctcatgctg cgagaacacc gcggcgagtt ccgcacgcag cgcctcgcga ttggcgatgc
     1741 ggtccttgtt gcgggcaaag cgcggatcac tgccgagttc ttccttgccg atttccttgg
     1801 cgagcttgcg gaaggtgccg tcattgccga cgccgatgaa gatgtcgtcg gtcttggtcg
     1861 ggaagatcgc gtaaggcacg agattcggat gctcgttgcc ggtgaggctc ggcggcttgc
     1921 cgtgcatgaa ataattcgcg gtgtgcggat gcatgatggc aaggccggtt tcgtagagcg
     1981 tggtctccag gaactggccg agcccggatt tctggcgctc cgacagcgcc atgaggatgc
     2041 cgatcgcggc ataaagtccg gtggtgatgt ccacgagcgg aacgccgatc cgcatcggcc
     2101 cgctctcctt cgagccggtc gcggcgatca tgccggtcat cgcctgaatg atggcgtcat
     2161 agccgggatt gccgccgcgc gggccgtcgc cgccaaagcc ggaaatccgg caatgcacca
     2221 gccgcggaaa attttgacgc aggacgtcgt tgccgatgcc ccatttgtcc agcgtgcccg
     2281 gcttgaaatt ctcgatcagg acgtcggccg tctcgagcag cttcatcagc accgcgcgac
     2341 cgccggcgga tgcgagatcg aggccgatcg agcgcttgtt gcggttgacg ccgacgaaat
     2401 aggccgcatc ctcctcgtgg aagggcggac cccaatcgcg ggtttcgtcg cccgcgggcg
     2461 gctcgacctt gatcacatcg gcgccgtggt cggcgaggat ttgcgtgcaa tagggaccgc
     2521 caagcacgcg cgaaagatcg atcactcgca atccggtcat ggcaccgggg gcgggggcag
     2581 cttcggtcgt catggctcaa tcttgtgagg ttcgtgggaa cgctccagcc attaagcacc
     2641 tcccgctgcc tgcacgcaaa tgcgctcccg caaaggccgc acggcaagcg catgccgtcg
     2701 cgcgtccacc attgcccgcc cggctgcacc gggcgagcaa tgtttcgcta gcctcagacg
     2761 atcttcagac ggacgtcgac gttgccccgt gtggcattgg aataggggca gacctggtgc
     2821 gccttggcca ccagagcttc agcctccgcg cgcgcgagtc ccggcagcga aatgccaagc
     2881 tcgatatcca ggccaaagcc accttccgag cgcggcccga tgccgacgat cgaggtcacc
     2941 gaggcgtcgg ctgggacctt tgggccacct tgggaagcga cgaatttcat tgcgccgatg
     3001 aagcaggcgg catagccggc agcgaagagt tgctcggggt tattgccggc tccgccggct
     3061 ccaccgagtt ccttgggggt agcgagcttg acgttgagcg ttccatccag cgtgccagcc
     3121 tggccgtcgc ggcctccggt cgccttggcg ctggttttgt aaagcacgtt tacgggcatt
     3181 gcaggctcct ttcttttttc ggctcgcgca aggtgtattg cgtgaaatta tattgtgctc
     3241 aatataaatt ataaggcttc acatttaatt gttcgcaatt gattgcgtcc gcacgccgcc
     3301 ccacaatcac ggcaggaatg ctgatgagga taccgatggc caggaagcat gcatccgagg
     3361 tgccgctgca gttgggcagc cagctctgct ttgcgatcta ttccgccgcg catgcgttca
     3421 accgcgtcta caagcctctg ctcgaccggc tcggcctcac ctatccgcag tatctcgtca
     3481 tgctggtgtt gtgggagcgc gacggcgtgc cggtcaagga catcggcgaa aggctgtttc
     3541 tggattccgg gacgctcacg ccgctgctca agcgcctcga ggcggcgcat ttgatcaagc
     3601 gcgcgcgcag cgcagaggac gagcgccagg tcctgatctc gctgacgtcg cagggccacg
     3661 cactgaagga gaaggccagg gccctgccgc ag
//
LOCUS       DATLJT010000912         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4195675,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000912 DATLJT010000000
VERSION     DATLJT010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4195675"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VM910_20975"
     CDS             <1..504
                     /locus_tag="VM910_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI65034.1"
                     /translation="ETSAIAAYVDEAFDGPTLQPKDVRARARMNQWISAVNSYYYPYM
                     IYHVSHERRVFPELGISSDEKVVAHALPKIEVGLQVLERELGHGKDHLLGSDLTLADF
                     YVLPSTYAFGLTKEGQGIYPKYPAFCRWRERMEAHPTVRKFRAALPPHAPIEHARQWA
                     VSHRPKY"
     gene            549..1013
                     /locus_tag="VM910_20980"
     CDS             549..1013
                     /locus_tag="VM910_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HVI65035.1"
                     /translation="MKWITREHVKVDRVACPWLIKKFVDKDAEFIFVPPDKVVDEAKR
                     LSAILYDVKNVELGHHGKECSFEAILKKYKLTGDPALVLLGKIVNGADTDNTLYQQPE
                     AAGLKAVAEGFRHLGFKDDHAVNAAEWIVYDALYAYCREMVRQGKHNGDFAP"
     gene            1067..1432
                     /locus_tag="VM910_20985"
     CDS             1067..1432
                     /locus_tag="VM910_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65036.1"
                     /translation="MRRSHPSILLAGSLVMFSAMQAVADQLCKPVLSLRDVRMSEARS
                     MQRTWTGVLVADASPCSTTSGPFEIEFTRLKETAPDLRFTERFTWTSGRSVVSIDVWW
                     DEWVEDYRIARIASCPCHD"
     gene            complement(1471..2259)
                     /locus_tag="VM910_20990"
     CDS             complement(1471..2259)
                     /locus_tag="VM910_20990"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVI65037.1"
                     /translation="MAEPSRPARLWYQSFVHPIEQAPYIERLQALLDAAAGPGVRFAV
                     HGLDPPDHLFHPLTEFRCAAQTIRNALDAERAGYDGFVIGHFQEPGLLEIRGAVDIPV
                     VGLGEATLLATLSMGRQLGLVTIDPIFIDWHERQVRAHGLEQRVTGVRAIHVDLPGFM
                     RAFTDDASYAQVRADFVEQVGPLVAAGAEIIIPAGGLPMLLFARERPFIIDGALVLNG
                     IAVVAKAAEMALALRRLTGSVVSRRGTYAKASAACVEEYLARRW"
     gene            complement(2335..>2608)
                     /locus_tag="VM910_20995"
     CDS             complement(2335..>2608)
                     /locus_tag="VM910_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65038.1"
                     /translation="AISVVIRQSLVQMRTPYEMLGRVLAVNSMFTGSSGSLGEFRAGA
                     VAAALGAVPSALIGGLGTIAVMLIWMWAFPQLRRVDKLTPEAGAAP"
BASE COUNT          512 a          825 c          820 g          451 t
ORIGIN      
        1 gaaaccagcg cgattgctgc ctatgtcgac gaagcgttcg acggccccac gctgcagccc
       61 aaggacgtgc gcgcacgcgc gcgcatgaac cagtggatca gcgccgtcaa ctcctactac
      121 tacccctaca tgatctatca cgtgtctcac gaacggcggg tatttcccga gctcggcatt
      181 tcgtccgacg agaaggtcgt cgcgcatgcg cttccgaaaa tcgaggtggg ccttcaggtc
      241 ttggaacgcg agctggggca cgggaaggat catttgttgg ggtccgatct cacgctggcc
      301 gacttctacg tgctgccgag cacgtatgcc ttcggactga ccaaggaagg acagggcata
      361 tatccgaaat acccggcctt ctgccgatgg cgggagagga tggaggcaca tccgaccgtg
      421 cgaaagtttc gtgccgcgtt gccgccgcac gctccgatcg agcatgcgcg gcaatgggcg
      481 gtttcgcatc ggcccaaata ctgatgggca cgccgcggcg ttcggatgca atgccaatgg
      541 aggatcgcat gaaatggatc acgcgcgagc acgtcaaggt cgaccgggtc gcctgcccat
      601 ggctgatcaa gaaattcgtc gacaaggacg cggagttcat tttcgttcca cccgacaagg
      661 tggtggacga ggccaaacgc ctgagcgcca tcctctacga cgtgaaaaac gtcgaattgg
      721 ggcaccacgg caaggagtgc tcgttcgagg cgatcttgaa aaaatacaag ttgaccggtg
      781 accctgcgct cgtgctgctg ggcaagatcg tcaacggtgc cgacaccgac aataccctct
      841 atcagcaacc cgaagcggcg ggccttaaag ccgtcgcgga aggtttccgc catctcggct
      901 tcaaggacga tcacgcggtg aatgccgccg agtggatcgt atacgacgcg ctttacgctt
      961 attgccggga gatggtgcgg caaggcaagc acaacggcga tttcgctcct tagatccacg
     1021 aacgcgatga cggcgcgctg gtcggtgatc gcgggagacc catcacatgc gtcgctctca
     1081 cccatctatt ttgctggcgg gtagcctcgt catgttcagt gcgatgcaag ccgtcgccga
     1141 tcagctctgc aaaccggtgt tgtcgttacg ggacgttcgc atgtccgagg cgcggagcat
     1201 gcaaaggaca tggaccggcg tcctcgttgc cgatgcatcg ccttgttcga ccacgtccgg
     1261 cccattcgag atcgagttca cccgcctcaa ggagaccgcg cccgatttgc gtttcacgga
     1321 gcgattcacg tggacttcgg ggcggtccgt ggtgtcgatc gacgtatggt gggacgagtg
     1381 ggtggaggat tatcggattg cccgcatcgc gtcgtgccca tgtcacgact agcgtccccc
     1441 ctttcgccga tgtcgtgatg gcgggtagat tcaccagcgc cttgccaggt actcctccac
     1501 gcatgccgcc gatgccttgg cgtaagtacc gcggcggctg accaccgagc cagtgaggcg
     1561 gcgtagcgcc agcgccatct ctgcggcctt tgccaccacc gcgataccgt tgagcacgag
     1621 cgcgccatcg atgatgaacg ggcgctcgcg cgcaaacagc agcatcggca ggccgccggc
     1681 cgggatgatg atctccgccc ccgccgcgac cagcggacca acctgttcca cgaaatcggc
     1741 gcggacctgg gcatagcttg catcgtcggt gaaggcgcgc atgaaaccgg gcaggtccac
     1801 atgaatggcc cgcacgccgg tgacgcgctg ctcgaggccg tgcgccctca cctgccgctc
     1861 atgccagtcg ataaagatcg gatcgatggt caccagacca agttggcgcc ccatgctcaa
     1921 tgtcgcgagc agcgtcgctt caccgagacc aaccacggga atgtcgaccg cgccgcggat
     1981 ctccaacaag ccgggctcct ggaagtgacc gatgacgaag ccgtcgtagc ctgcccgctc
     2041 cgcgtcgagc gcattgcgga tcgtctgcgc cgcgcagcgg aattccgtca gcggatggaa
     2101 gaggtggtcg ggaggatcga ggccatggac cgcgaaccgg acgccaggac cggcagccgc
     2161 gtcgagcaac gcttgcagcc gctcgatgta gggcgcctgc tcgatcggat gaacgaagct
     2221 ctgataccac agccgggcag gacgggaagg ttcggccatg cgctatcccc cagcgtcaat
     2281 gatgcgcggt cttggacagg aacgagcgca ccttaatcgg tcgcaatgtc gcggtcacgg
     2341 cgccgctcct gcctccggcg tcaatttgtc gacacggcga agttgcggga acgcccacat
     2401 ccagatcagc atcaccgcga tcgtgcccaa tccgccgatc agcgcggacg gcaccgcgcc
     2461 cagcgcggcc gcgacggcgc cggcgcgaaa ttcgccgagc gagcccgagg agcccgtgaa
     2521 catcgagttg accgccagca cccggcccag catctcgtag ggggtgcgca tctgcaccag
     2581 cgattgccgg atgacgacgc tgatcgcg
//
LOCUS       DATLJT010000913         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_60215,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000913 DATLJT010000000
VERSION     DATLJT010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60215"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1041
                     /locus_tag="VM910_21000"
     CDS             316..1041
                     /locus_tag="VM910_21000"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI65039.1"
                     /translation="MALIGATIVFSSFLSGVFGMAGGMVLLGVLLNYFDVATGMILFS
                     IIQLFANGWRVLQWRRYVLWPIFGWYVVGAAISFAGMWMIAFVPDKAIVYLALGLMPF
                     VVEALPAAMRPNIEWRGVPFITGVATTIIQILAGVGGLFLDIFFQKSMLDRKTTNATK
                     AVAQSFSHVVRAFYFGSLSGVGDLPLWWTGPAILLAIGGTSLAPFVIERMTDHGFRQW
                     TRAIILALSTVYLVRAGWLFWNR"
     gene            complement(1100..1720)
                     /locus_tag="VM910_21005"
     CDS             complement(1100..1720)
                     /locus_tag="VM910_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI65040.1"
                     /translation="MTTLQTVRREASCACCSDLLNGKFDRRHFVQAVAVGAATFGLVP
                     YIALAAEGNYEAMVLSCIDPRMQEPVHKYTVEQNLTGKFSQFVIAGAAIGVVAPAFKD
                     WHKAFWDNLGTTIQLHHIKRVIAIDHRDCGAARIAYGEAKVANPQVETETHRAALAEF
                     RKQVKERHPQLGVETGLMALDGKLEMFQLTVWASRYYTITLQRRCA"
     gene            2303..>2637
                     /locus_tag="VM910_21010"
     CDS             2303..>2637
                     /locus_tag="VM910_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HVI65041.1"
                     /translation="MARTIYKICPNVLWREAERAGVFRGAPVDARDGFIHFSSATQVR
                     ETATRYFAGAEDLMLIAVDADALGDALKWQVSRGGDLFPHLYGDLPLGAVLWVRPLPL
                     GTDQQHVFP"
BASE COUNT          441 a          804 c          804 g          588 t
ORIGIN      
        1 ttgcgctcga ttggttcaac atgctctatc aggtgctgct cggccaggag aagggtccgc
       61 gcttcggctc gttcgtggcg gtgtacgggc ttcagaacac gatcgacatg atcgacggcg
      121 cgctggcgag atcggcataa aacgcgtcgc tttcatttgt gccgaggtcc gacacacgcc
      181 gcttgccgtc atcgccaatg ttgcacatcg ctcgctgccg cgaccggcgc cggctggctg
      241 tccttgcggg caaaccgcag gtgagggaca ctgggccgtt gtcctaagct cattcccgca
      301 atggcacctt ccgtcttggc tcttatcgga gccaccatcg tcttttcttc atttctctcc
      361 ggcgttttcg gcatggccgg aggcatggtg ctgctcggcg ttctactcaa ctatttcgac
      421 gtcgccactg ggatgattct tttttcgatc atccagctgt tcgccaatgg ctggcgcgtt
      481 ttgcagtggc gcagatatgt gctgtggccc attttcggtt ggtatgtggt cggcgcggcc
      541 atctcctttg ccggcatgtg gatgatcgcg ttcgttccgg acaaggcaat agtctacctc
      601 gcacttggtt tgatgccgtt cgtggtggag gccctgcccg ccgccatgcg cccgaatatc
      661 gagtggcggg gtgtgccgtt catcaccggc gtggcgacca cgatcatcca gatcctggcc
      721 ggcgtcggcg ggctctttct cgacatcttc tttcagaaga gcatgctcga ccgcaaaacg
      781 accaatgcca ccaaggccgt cgcgcagtca ttcagccacg tcgtgcgcgc gttctatttc
      841 ggctcgctct cgggcgtcgg cgacctgccg ctgtggtgga cgggaccggc gatcctgctg
      901 gcgatcgggg gcacgtcgct cgcacccttt gtcatcgagc ggatgaccga tcacggattt
      961 cggcagtgga cgcgcgcgat catcctcgcc ctcagcacgg tctatctcgt ccgtgccggc
     1021 tggttgttct ggaatcgctg aggcggcgcg ggagcatccg cgttcgcccc agcgcaacgc
     1081 gttcccaacg tttgcatttt cacgcgcagc gccgctgcag cgtgatcgtg taatagcgac
     1141 tcgcccacac ggtcagctgg aacatctcca gcttgccgtc gagcgccatc aggccggttt
     1201 cgacgccgag ctgcggatgg cgctccttga cttgcttgcg gaattcagcg agcgccgccc
     1261 tatgcgtttc ggtctccact tgcgggttcg cgaccttggc ctcgccataa gcgatcctcg
     1321 cagcgccgca atcgcgatgg tcgatcgcga tgactctctt gatgtgatgc aactgaatcg
     1381 tcgtgccgag attgtcccaa aatgccttgt gccagtcctt gaacgcgggc gcgacgacgc
     1441 cgattgctgc accagcaatc acgaattggc tgaacttgcc ggttaagttc tgctcgaccg
     1501 tatatttgtg caccggctcc tgcatgcgag gatcgatgca ggacagaacc atcgcctcat
     1561 agttcccttc agctgcaagg gcgatgtatg ggacgagccc gaaggtcgcc gcgcccaccg
     1621 cgacggcctg tacaaagtgc cggcggtcga atttgccatt cagaagatcg ctacaacatg
     1681 cgcaggaagc ctcacgtcga accgtttgca atgtcgtcat tgtccctctc ttggcgttgg
     1741 tctcatgcac atcgagcttt tgtggaggca tgtccgttcg cgctcatact acacatgtgc
     1801 gggcgacaaa agtacgcaac cgttgccggt gacgtgggcg ggatcgagcg cagcgcgatc
     1861 aagcttgccg tgtgctactc caccacgcgg tagcggcggc gaacaatcgg caatccgcga
     1921 cacatgacga aagtgcttgg atgataggcc ggtgtcttcg aggcgccgga aatctctacc
     1981 gctaggcgtg cccgtggccg gcgctggggg tctcgtccca gtccggaccg acgggaaaac
     2041 ccacgtcgaa gtcgccatct ttcgctggcc tcggccaacc cgatcatcgg gaccgttttc
     2101 ccgctatttg caaccgccgc gatcgaagtc aaagaatgca atctggagtg caaatgcgct
     2161 tgccgtggtg gttgccgagg gccggactca agcgccgctg cgcttgaggt tagcggcgca
     2221 gtcggagtta actggacgtc atactttggc ggacgggatt gatctcaggg acatccaatc
     2281 tcgcggccga acgcggggcg ccgtggcgag gaccatctac aagatttgcc cgaacgtgct
     2341 ttggagagag gcggagcgtg ccggtgtatt ccgcggtgcg cccgtcgatg cgcgcgacgg
     2401 cttcattcac ttctcatccg ccacgcaggt ccgcgagacc gcaacgaggt attttgccgg
     2461 ggctgaggat ttgatgctga tcgcggtcga cgcagacgcg ctcggcgatg cgttgaaatg
     2521 gcaagtctcg cgcggcggtg atttgtttcc gcatctttac ggcgacctgc ccctcggtgc
     2581 cgtgttgtgg gttagaccgc ttcccctcgg gaccgatcaa cagcacgtct ttccgga
//
LOCUS       DATLJT010000914         1534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_977664,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000914 DATLJT010000000
VERSION     DATLJT010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977664"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..391)
                     /locus_tag="VM910_21015"
     CDS             complement(41..391)
                     /locus_tag="VM910_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65042.1"
                     /translation="MEPESKNRDLVHVEASWTPKTETDVDIAPLIQKVGATSIAEIEK
                     LIGELQQAKHFLQTEGERIRRETVRYIKFAQLGSASVKVLFDTVSGWRQEGHPLRKFS
                     TGSHGQLFDSAPDG"
     gene            complement(1034..1240)
                     /locus_tag="VM910_21020"
     CDS             complement(1034..1240)
                     /locus_tag="VM910_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65043.1"
                     /translation="MARKRYKPEEIVAKLRQVDVLVSQGQGMTEAIHLQPAKFLTPTI
                     VGHLAHTDLPVPRIAIATMKLRLG"
BASE COUNT          283 a          465 c          462 g          324 t
ORIGIN      
        1 aacgccgcac ttgccgcgtg gttcggtgca ttcagttacg ttacccgtcg ggcgcggaat
       61 caaagagctg gccatgcgaa ccggtgctga atttgcgcaa ggggtgaccc tcctggcgcc
      121 agccggagac ggtatcaaag aggactttta ccgaggctga acccaactga gcgaatttta
      181 tatagcgaac cgtctctcgc cgaattcgct ccccctcggt ttgcagaaaa tgttttgctt
      241 gttgcagctc cccgatcagc ttttcgattt ccgcaatcga tgtagctcca accttctgaa
      301 taagtggcgc aatatctacg tcagtctcgg tcttgggcgt ccaagacgct tctacgtgaa
      361 cgagatcgcg gtttttgctc tcgggttcca taacacccgc tccttcccct cctgaaatcc
      421 aagtttcacg cgaggaagtt aatacggcat gaatgggccc tcatcttcgg ccagatcgcg
      481 tcatacagta ataggcgctc agtgccgtac gatccgagag gaagtgaagc agcgcggccg
      541 aaaataaatc tcttgaaatc aagccgaact cgcggaccgg gcggcgctcc gttccagcct
      601 gtgcccggcg tgcaccccgt ccgcgccggg tgccgcgctg ttgggacagc acgctcgtgg
      661 cgtggtatcc ttccgtttac gcacctcgca cgggaggggc gtatgacagt caccatcgga
      721 cggcgggaat tgctggctgc ggcgcggcgg ccgcgtggcc gctcgcggac cgcatgcggc
      781 gcatcggcgt gctcatgaat cgcgccgcgg acaaccccga agggcagacg cgcctggcgg
      841 cgttcatgca ggggctgcag gaagcgggct gggattcatg aatccggcgt ttctcattct
      901 gtacccacat ttgtacccac atttcgacgt cgagatcgcg cgcgcggtac cgcgcgcgag
      961 ggcaggaaga gcccatgcca tgggcctgga ccccgccccc accctggaca ggtcggcccc
     1021 cctggtcgac tccctagcca agccgcaatt tcatcgtcgc tattgcgatc ctggggaccg
     1081 gcagatcggt gtgagcgagg tgacctacta tcgttggcgt caagaatttg gcgggctgaa
     1141 gatggatcgc ctccgtcatg ccttggccct gcgagactag aacatccact tggcggagct
     1201 tcgcgacgat ctcttcaggc ttgtagcgct tccttgccat tgcagtcctc ggaagggctg
     1261 gcatttagca tcgtcatggc tgcaccctct agagtcgccc agtcgtcgtt tgggatcgat
     1321 gtccgcgctc cacgccccgt tgtgtcgtga caagctccgg cccgtcacgg cgtcactcgg
     1381 cggcggagcg ctggaacggg ttagcgctca gagatggcgg actcgctgct tgatgcctcg
     1441 aaggaagcct tcgcatattc gagaagcacc ggatattttt gctcagttgc agcggatcaa
     1501 gccgcggagg gttatcgcca aggtcgagtg gcat
//
LOCUS       DATLJT010000915         1835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_747309,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000915 DATLJT010000000
VERSION     DATLJT010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747309"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1114
                     /locus_tag="VM910_21025"
     CDS             44..1114
                     /locus_tag="VM910_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVI65044.1"
                     /translation="MHIAQVAPLTEAIPPKLYGGTERVVSWLTEELIALRHEVTLFAS
                     GDSMTSARLEAVWPRALRLDGSVRDPNALHMLMLERVYQSAADFDFIHCHLDYYPFSL
                     FSRQPTPFVTTLHGRLDLPEHQPVFDQFSSIPVVSISNAQRRPLPQVHWVRTVHHGLP
                     ADLLVPKPIKPSYFAFLGRIAPEKRVDRAIRIAEHCGVPLKIAAKVDKVDQDYFNEQI
                     KPMLGNGNCEYIGEISDREKAEFLSGAIVLLVPIDWPEPFGLVMIEAMACGTPVISFN
                     RGSAPEVVDDGLTGFIVEDVNGAIGAIDRLGHLSREKVRRRFEERFTAGRMAQDYLSV
                     YRTLMTAAAPHLRLVADEAPMR"
     gene            complement(1331..>1835)
                     /locus_tag="VM910_21030"
     CDS             complement(1331..>1835)
                     /locus_tag="VM910_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65045.1"
                     /translation="VGSVVTMRSARWATLIIAACIVVPQLVVAILSPWVGMRAQIWGR
                     RPLLLIGFAALPIRGLLLTFVSDPSLLIAVQILDGVTASVFAVMVPLVVADLTRGTGH
                     FNLGQGILGTATGIGASLSATLAGYLTDHFGSAAAFGSLATIALAGFTLVWFLMPETR
                     PAKLSEP"
BASE COUNT          348 a          571 c          579 g          337 t
ORIGIN      
        1 tagtcgccgg ggtgaggaat caacagaatc gagggacttt tcgatgcaca ttgcacaagt
       61 ggcgccgttg accgaagcca tcccgccgaa gctctatggc ggcaccgaac gggtcgtttc
      121 ctggctgacg gaagagctga tcgcgctgcg gcacgaggtg accctctttg ccagcggcga
      181 ttcgatgacg tcggcgcggc ttgaagcggt gtggccgcgg gcgctgcggc tcgacggctc
      241 ggtccgcgat cccaacgcgc tgcacatgtt gatgcttgag cgcgtctatc agtcggcggc
      301 cgatttcgat ttcatccact gccacctgga ttactacccg ttctcgctgt tttcgcggca
      361 gccgacgccg tttgtcacga cgctgcatgg caggcttgac ctgccggagc accagccggt
      421 gttcgatcag ttctcctcga ttccggtggt gtcgatttcg aatgcgcaac gccggccgct
      481 accgcaagtg cactgggtgc gcaccgtaca tcatggcctt ccggccgacc ttctggtgcc
      541 gaaaccgatc aagccgtctt acttcgcgtt tctcggccgc atcgcgcccg aaaaacgggt
      601 tgatcgggcc attcgcattg ccgagcattg cggcgtgccg cttaagatcg ccgccaaggt
      661 cgacaaagtc gaccaggatt acttcaatga gcagatcaag ccgatgctgg gcaacggcaa
      721 ttgcgaatat atcggcgaga tcagtgaccg ggaaaaagcg gaattcctga gcggcgcgat
      781 cgtgctcttg gttccgatcg attggccgga accgttcggc ttggtgatga tcgaggccat
      841 ggcctgcggc acgccggtta tctcgttcaa tcgcggttcc gctccggaag tggtcgacga
      901 cggcctaacc ggcttcattg tcgaggacgt caatggcgcc atcggtgcga tcgatcggct
      961 cgggcacttg tcgcgcgaga aggtgcgccg ccgattcgaa gagcgcttca cggcggggcg
     1021 catggcgcag gactatctga gcgtctaccg caccctgatg acggcagccg cgccgcatct
     1081 gcggctggtc gccgacgaag cgccgatgcg ctagcgccta attcgcggcg atcgcgactg
     1141 gctgtcgctt gcaattccgt tcgtccccgc gacgacccgc ctacgctcgc ttggtgagct
     1201 ctggcgggtt cggagtccgc caaagcgcgg agtgcgaagg cagaaagcgc ggaccgagct
     1261 tggatcttcg ggacaaactc ccgcttacgc gggaatgagc ggatgtttta acgccgtcgc
     1321 ggccaaggtc ctagggttca ctcagtttcg ccggccgcgt ttccggcatc aaaaaccaaa
     1381 cgagcgtaaa gccggcgagc gcgatggtcg ccagactgcc gaaagctgcc gcgctgccga
     1441 aatgatcggt caaataaccc gccaaagtcg cgctcagcga cgctccgatt cccgtggcgg
     1501 tgccgagaat gccctgtccc agattgaaat ggccggtacc gcgcgtgagg tcggcgacca
     1561 caagcgggac catgaccgcg aataccgacg ccgtgacccc gtcgagaatc tgcacggcga
     1621 tcagcagtga tgggtcggag acaaaagtca gtaacagccc gcggatcggc agcgcggcaa
     1681 agccgatcag aagcagcggc cgccgtcccc agatttgcgc gcgcattccg acccacggtg
     1741 agaggattgc caccacaagc tgcggcacca cgatgcaggc agcgatgatc agcgtggccc
     1801 agcgcgccga tctcatcgtc acgacgctgc cgacc
//
LOCUS       DATLJT010000916         1749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3277151,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000916 DATLJT010000000
VERSION     DATLJT010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277151"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VM910_21035"
     CDS             complement(<1..1259)
                     /locus_tag="VM910_21035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI65046.1"
                     /translation="MKGCNIEIALKVRPRMSLLAAVVTLSGAFFSAQAQEPIKIGFGV
                     SLTGGLASSGKAHLMSKQIWAEEINAKGGLLGRPIKLVYYDDQTNAATVPGIYAKLID
                     VDKVDLLMGHATNLIVAAMPIIIERKKLVMVLLALGSNAEFKYPRYFQSAAFGPDSKG
                     VLSNNFLAVAKSLAPEPKTVALVGADAEFSNNVLIGARENAKKYALKIVYDRSYPPAT
                     VDYTPIVRAIQATSPDVVLVASYPPDSVGIVRAATEIGLKTQLFGGAMVGMQYASLMQ
                     QLGEKLNRVVNYHLYVPSAKMNFPGIEAFLKKYQAQAKEAGTDPLGFYQPPFAYAAMQ
                     VLEQAIKATGSLNDDKLAGYIHNNAFNTIVGEIRFNELGEWATARMLLVQFQNVQGSG
                     LDQYMTGHKQVILYPPEYKDGELEAPFA"
BASE COUNT          310 a          534 c          554 g          351 t
ORIGIN      
        1 gcgaacggag cctccagttc gccatctttg tactcggggg gatagaggat gacctgcttg
       61 tgaccggtca tgtactgatc gagcccgctg ccttgaacgt tctgaaactg caccagcagc
      121 atgcgtgccg tcgcccattc gccgagttcg ttgaagcgga tttcgccgac gatcgtgttg
      181 aatgcgttgt tgtgaatgta gccggcgagc ttgtcgtcgt tgaggctgcc ggtcgccttg
      241 attgcctgct ccagtacctg catcgcggca taggcgaagg gcggctggta gaagccgagc
      301 gggtcggtgc cggcctcctt ggcctgcgcc tgatacttct tcaggaacgc ctcgatgccg
      361 gggaaattca ttttggcgct agggacatag agatggtagt tcacgacgcg gttgagcttt
      421 tcgcccagct gctgcatgag cgacgcgtat tgcattccga ccattgcccc accgaacaac
      481 tgggtcttca ggccgatttc ggtcgccgca cggacgatac cgacggaatc gggcgggtag
      541 gacgcaacca gaaccacgtc ggggctggtc gcctggatgg cgcgcacgat cggcgtgtag
      601 tcgaccgtcg ccggcggata gctgcggtcg tacacgatct tgagagcgta ctttttcgcg
      661 ttctcgcgcg cgccgatcag cacgttgttg gagaactcgg cgtcggcgcc gaccagcgcc
      721 acggtcttgg gctccggcgc gagcgatttg gcgaccgcga ggaagttgtt cgacagcacg
      781 cccttggaat cgggaccgaa ggctgcgctc tggaaataac gcggatactt gaactccgcg
      841 ttcgagccca gcgcgagaag caccatgacc agcttcttgc gctcgatgat gatcggcatc
      901 gccgcgacga tgaggttggt cgcgtggccc atcagcaggt cgaccttatc gacgtcgatc
      961 agcttcgcgt agatgccggg caccgtcgcg gcgttggtct ggtcgtcgta gtagacgagc
     1021 ttgatcggac ggccgagcag cccgcccttc gcgttgatct cctcggccca gatctgcttg
     1081 gacatcagat gggccttgcc cgaggaggcg agcccgccgg tgagcgatac gccaaagccg
     1141 atcttgattg gctcctgcgc ctgcgccgaa aagaacgcgc cggacaatgt gaccaccgcc
     1201 gccagtagcg acattcgcgg ccgtactttc agcgcgatct cgatgttgca ccctttcatc
     1261 gcgtcctccc cggtacatca aacgagccaa cgcaccgtgc gttcggtgcg gacgatacta
     1321 gcgatttgct gagtgctcga aagccaagat gcgagatggg accgtgcccg ccgttgcaac
     1381 gggccgcgaa ttgcgtgggc gccgacagcg gaacactatc ttttgcattc tcgcgtgctt
     1441 gtctccacgg agcggaggca tgcgatcgtt cttgagttgg gcaggctgga cctccgcgcc
     1501 cggtctccag acccgcgacg cgacgggtac cgggcctccc cgtaccatcg gttatgcgag
     1561 cgtcaagatc atgcaggcaa cgcttcggac gcgcgcggga aacggctcat ccttctgttt
     1621 cagaaaaatg cgcaagaatc tgaaggacac ccgctattgg caaaccggtt agcctgggct
     1681 cgtgccaccc ctgctagtcg ccgcgcggtg ccgcccgcaa gcgccgatgg ccgtccgcac
     1741 gaccttgcg
//
LOCUS       DATLJT010000917         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3277211,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000917 DATLJT010000000
VERSION     DATLJT010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277211"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VM910_21040"
     CDS             complement(<1..198)
                     /locus_tag="VM910_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI65047.1"
                     /translation="MATLHTRSVARIMVAPSVALLFLWMAVPLAMTLYFSFIRFNLLI
                     PGPRSFIGFDNYYFFLTDPAFA"
     gene            complement(516..1802)
                     /locus_tag="VM910_21045"
     CDS             complement(516..1802)
                     /locus_tag="VM910_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVI65048.1"
                     /translation="MIGAIAIARPSPGDTTLTIATVNNGDMIRMQKLTDDFKKRNPGI
                     DLEWVTLEENVLREKVTTDIATKAGQYDVLTIGTYEVPIWAKQGWLVPLSLGADYDVG
                     DLLPPIRSALSYDGKLYAAPFYGESSMLMYRKDLFAKAGLTMPEKPTWDFIKTAADKI
                     TDKNNEIYGICLRGKAGWGENMALLTTMANSYGGRWFNEKWEPQFTGPEWKTALETYL
                     DLMKKDGPPGASSNGFNENLALFQAGKCGMWIDATVAASFVSDPKQSKVANEVGFAPA
                     PCGTTCKNSNWLWAWALAIPSSSKKIDAAGKFIDWATSKEYIDLVASKNGWANVPPGT
                     RKSLYANPDYTKAAPFAEMTLKAINSADTGLDGNSKPKPYIGIQYAAIPEFQGIATAV
                     GQQFSAALVGQTSPGQALDNAQRLSERDMKRAGYIK"
     gene            complement(1938..>2658)
                     /locus_tag="VM910_21050"
     CDS             complement(1938..>2658)
                     /locus_tag="VM910_21050"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HVI65049.1"
                     /translation="DLKYCDVVPSDPASTSTVLGVAEAAAAYLERILGSPTPLILALG
                     TGRTIRASVDQLPQMDCRQHKIVSLVGNIAPDGSASFFDVITKISNTVKAPHFPMPVP
                     VVASSVEEKAVLLRLKPVKHVCDLAKRADVTLVGIGSLDDNAPLFEDGFITRGELRAL
                     RKAGAIGEIVGWAYDADGQIVAGHTNQRVVSVPIGKVGQATVIGAAMGPAKMAAIRAA
                     LVGQLINGLITDETTARALTG"
BASE COUNT          486 a          829 c          806 g          537 t
ORIGIN      
        1 cgcgaaggcc gggtcggtca gaaagaaata atagttgtcg aacccgataa acgatcgcgg
       61 gccagggatc agaagattga atcggatgaa cgagaaatac aatgtcatgg ccagcggaac
      121 ggccatccac aggaacagca gtgccaccga cggcgcgacc atgatccgcg cgacggagcg
      181 tgtatggagt gtggccattc cggcaacctg cttaacggtt cattgcctaa attgagggat
      241 gcgctgaggg cattgatggg tcggagtctc tatgctgcca ctcctcgctt gctgagagag
      301 cccggattgc cgctcgcgaa ggggggcgtt ccgagggtgg ggtgaggggc aattggcgac
      361 ccccagcagc cgcaaatagc ctcggaaaat ccgctcaccc ggaagccgcg ctacgcgccg
      421 cttccgacct ctccccgcaa gcagcgtggg ataatcaacg gactggttct ctgccatcgg
      481 cccgcaccgc gcgatagccg accctaacag ccttgctact tgatgtaccc ggcccgcttc
      541 atgtcgcgct cgctcagccg ttgcgcgtta tcgagtgcct gacccggtga agtctggccg
      601 accagggctg cggaaaattg ctggccgacg gcggtcgcga ttccctggaa ctcgggaatc
      661 gcggcatatt gtatgccgat atagggcttc ggcttggagt tgccgtcgag accggtatca
      721 gctgaattga ttgccttcag agtcatttcg gcgaaaggcg cggccttcgt atagtccgga
      781 ttggcataaa gcgatttgcg cgtacccggc ggcacgttgg cccagccgtt tttcgacgcg
      841 accagatcaa tgtattcctt gctggtcgcc caatcgataa acttcccggc cgcgtcaatt
      901 ttcttcgagc tgctcggtat cgccaacgcc cacgcccaca gccagttgga gttcttgcag
      961 gtcgtgccgc acggcgcagg cgcgaacccg acctcattgg cgaccttcga ctgcttcggg
     1021 tcgctgacaa acgacgccgc caccgtcgca tcgatccaca tgccgcactt gccggcctga
     1081 aacagcgcca gattctcatt gaacccgttc gacgaggcgc ccggcgggcc atccttcttc
     1141 atgagatcca gataggtctc gagcgcagtc ttccattccg gaccggtaaa ctggggttcc
     1201 cacttctcgt tgaaccagcg cccgccatac gagttcgcca tcgtcgtcag cagggccatg
     1261 ttctcgcccc acccagcctt tccgcgcagg cagatgccgt agatctcgtt gttcttgtcg
     1321 gtgatcttgt cagctgccgt cttgatgaag tcccaagtcg gcttttcggg catcgtgagc
     1381 cccgccttcg cgaacaggtc tttgcggtac atcagcatcg agctttcgcc gtaaaacggc
     1441 gcggcgtaga gcttgccgtc gtacgacagg gcgctgcgga tcggcggcag gaggtcgcca
     1501 acatcgtaat cggcaccgag gctgagcggc accagccagc cctgcttggc ccagatcggc
     1561 acctcgtagg tgccgatcgt cagcacgtcg tactggcccg ctttcgtcgc aatgtcggtc
     1621 gtgaccttct cgcgaagcac attctcctcg agggtgaccc actcgagatc gattcccggg
     1681 ttccgcttct tgaaatcatc cgtcagcttc tgcatccgga tcatgtcgcc gttattgact
     1741 gtggcgatcg tcaaggttgt atcgccgggg ctggggcggg cgatcgcgat cgcgccaatc
     1801 atgacagcca gggtccacct catggcttcc tcccagtttt tgagcaaatg ctctaagtta
     1861 gggcgaatgc tcacacagct cgtagccggg tgtcaagcat gctcgcaaag agccgcggaa
     1921 ccgggcagcg cgctcgatca gccggtaagc gctctggccg ttgtttcgtc ggttatcagg
     1981 ccgttgatca gctggccgac cagtgcggcg cgtatcgccg ccatttttgc gggtcccatc
     2041 gcggcgccaa tcaccgtggc ttgaccgacc tttccgatcg gcacgctgac cacgcgttgg
     2101 ttggtgtggc cggccacgat ttgtccgtcg gcgtcgtagg cccagccgac gatctcgccg
     2161 atcgcgccgg ccttccgcaa agcgcgcagt tcgccgcggg tgatgaatcc gtcctcgaag
     2221 agcggcgcgt tgtcgtcgag gctgccgatg ccgaccaacg tcacatcggc gcgcttggcc
     2281 aggtcgcaca catgctttac gggcttcagc cggagcagga ccgccttctc ctcgacactg
     2341 ctcgccacca ccggaacggg catcgggaag tgcggcgcct tcacggtatt cgagattttt
     2401 gtgatcacgt cgaagaagct cgccgaccca tcgggcgcga tgttgccgac cagtgagacg
     2461 atcttgtgtt gccgacaatc catctgaggc aactggtcga ccgaagcgcg gatcgtgcgg
     2521 ccggtgccga gcgccaggat cagcggcgtc ggcgagccca aaatgcgctc gagataggcg
     2581 gcagccgcct cggctacgcc gagcactgtg ctggtcgagg cgggatcgga gggaacgaca
     2641 tcgcaatatt tcaggtcg
//
LOCUS       DATLJT010000918         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1209418,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000918 DATLJT010000000
VERSION     DATLJT010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209418"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VM910_21055"
     CDS             <1..702
                     /locus_tag="VM910_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI65050.1"
                     /translation="RIKGFYLAVATLASQFFLIWMFNKFPWFYNYSASGVISAPPRAV
                     FGQVYVTGAEADPRAKYLFVLTVVMLLALAAKGLVRGRVGRSWMAIRDMDIAAEIIGI
                     RPLRTKLLAFALSSFYCGVAGAMWAFIYTSAVEASAFEIDRSFQILFMIIIGGLGSIL
                     GSFLGAAFIVLLPILLDNAPRLAGFQVPVNLVTNLQSMIFGALIVFFLILEPNGLARL
                     WAIGKEKLRLWPFPH"
     gene            complement(933..1811)
                     /locus_tag="VM910_21060"
     CDS             complement(933..1811)
                     /locus_tag="VM910_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65051.1"
                     /translation="MAVTQVVLVDQTHEIDPGLLHNAALALDSQVTQDLPRYWSGITA
                     NVSAVPALSAVPHGAWPVFLVRSLPPGEGGFHMDRHNQPYAKVIASPDDDSWTVDASH
                     EIIEMLIDPYGNRMQLSEAIAISGNDVVDHAGTFNYLVEGCDPCEANNYAYDIAGIAL
                     SDFITPHFYDASVTPGTQYSFKGNLKRPRQLLPGGYISYMQPDGTWNQILWVNPGQPP
                     QYHNPSVAANSRSLREAIHLAMGAELDTAKHHQRRKQGGLPKAVNDRITEHRSRRAAR
                     GRYEEVLRERYLLGKS"
BASE COUNT          324 a          542 c          562 g          426 t
ORIGIN      
        1 aggatcaagg gtttctacct cgccgtggcg acgctcgcgt cgcagttctt cctgatctgg
       61 atgttcaaca agttcccgtg gttctacaac tacagcgctt cgggtgtgat cagcgcgccg
      121 ccgcgcgcgg ttttcggcca agtctacgtc accggtgccg aggccgatcc ccgggcgaaa
      181 tacctgttcg tgctgaccgt cgtcatgctc ttggcactcg ccgccaaggg tctggtgcgc
      241 ggccgcgttg gacgctcttg gatggcgatc cgtgacatgg acatcgcggc ggagatcatc
      301 ggcatccgcc cgctgcgcac aaagctgctc gccttcgcct tgagctcgtt ctattgcggc
      361 gtcgccgggg cgatgtgggc ctttatctat acgagcgccg tcgaagccag cgcgttcgag
      421 atcgaccgct cgttccagat cctgttcatg atcatcatcg gcggtctagg cagcatcctg
      481 ggttctttcc tcggcgccgc ctttatcgtg ctgctgccga ttcttctcga caacgcgccg
      541 cggcttgctg gatttcaagt tccggtcaat ctggtgacca atctgcaatc gatgatcttc
      601 ggtgcactta ttgttttctt cctgatcctt gagcccaatg gtctggcacg gttgtgggcg
      661 atcggcaagg agaagctacg gctctggccg ttcccgcatt gacgccgagg gacggataac
      721 gaaccgccgt cgcggcagca cgcgaccgcc gacggcgccc gcgagtgacc aggggcacgc
      781 cggctgccgc gatctttttg ctacctcttg tatttaaatt ctgtattagc tgatctaatg
      841 gtgcctgtct ctgtagatcc tccgatttca cggcacggat tgggcagcgc gccggatgcg
      901 gctgttcata cggcgcgctc gcccaatgat tatcaggatt tgccgagaag ataccgttcc
      961 cgcaacacct cctcgtaacg gcccctcgct gcgcggcgtg agcgatgctc ggtgatcctg
     1021 tcgttcacag ccttcgggag gccgccttgc ttccgccgct gatgatgctt cgcggtgtcg
     1081 agctcggcgc ccatcgccag atgaatcgcc tcgcgcaaag agcggctgtt cgcggcgacc
     1141 gacggattgt gatattgcgg cggctggccg ggattgaccc agagaatctg gttccacgtc
     1201 ccgtccggtt gcatatacga aatgtatccg ccgggcagga gctgtcgggg gcgcttgaga
     1261 ttccccttga agctgtactg cgtaccgggg gtcaccgacg cgtcgtaaaa gtgcggcgtg
     1321 atgaaatcgg ataaggcgat gccggcaatg tcgtaggcat agttgttggc ctcgcacgga
     1381 tcgcagcctt cgacgagata gttgaatgtg ccggcgtggt cgacgacgtc attgccggaa
     1441 atagcgatcg cttcggacaa ttgcatgcga ttgccgtacg gatcaatgag catttcgatg
     1501 atttcgtgac tggcatctac agtccagcta tcgtcgtcgg gagaagcgat cactttggca
     1561 tagggttggt tgtgccggtc catgtggaag ccgccttcgc cgggcggcag cgaacgcacg
     1621 agaaataccg gccaggcgcc atgcggaacg gccgaaagcg ccggcacggc ggagacattc
     1681 gcagttattc cgctccaata ccgcggcaaa tcctgcgtga cctgactatc cagggcgagc
     1741 gcggcattat ggagtagtcc tggatcgatt tcgtgggttt gatcgactag cacaacctgg
     1801 gttaccgcca ttttaccctc ctctgatagg attggcagat tctatccgta gaac
//
LOCUS       DATLJT010000919         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2128177,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000919 DATLJT010000000
VERSION     DATLJT010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128177"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..472)
                     /locus_tag="VM910_21065"
     CDS             complement(2..472)
                     /locus_tag="VM910_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HVI65052.1"
                     /translation="MFDSFRKFLSEASDDGAHPIRFEHNDYRLAAAALLVHAAAIDGD
                     ISDAERDQLHAVIKRQFDLDEATTDELVAEATEAEHEAIDLYHFTSLINRSLDEDGRR
                     RVVEMMWELAYADGRVDDFESNLIWRVADLLGISSRERIELGQRVASRRGSAPT"
     gene            complement(618..>1525)
                     /locus_tag="VM910_21070"
     CDS             complement(618..>1525)
                     /locus_tag="VM910_21070"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI65053.1"
                     /translation="STGYGFTEGLVWVAKGKSGYLLLSDMPANVIYKLSTDGKEKSLY
                     LDRSGYTGFDIWRVGFMQTNGRSKDDPRYEEFAMIGSNGLTLDREGRLVIATWAGRGI
                     DRIEKNGKRVTLTDNLDGKKFNGTNDLVVKKDGAIYFTDDTGGLRLRDQDPRRGIAFL
                     GVFRWKDGKTTAVIRDIPHANGLAFSPDEKILYANGSRERFVRAYDVQADGMLTNSRL
                     LIDLNSDPRPGITDGMKVDTKGNIWESGPGGIWIISPQGAVLGHIQVPELVANLAFGD
                     PDYKTLYIAARSSVYKIRTNVAGIP"
BASE COUNT          271 a          484 c          464 g          306 t
ORIGIN      
        1 gtcacgtcgg agcgctcccg cgtcggctcg ccacgcgctg gcccagctca atccgctccc
       61 gcgacgagat gccgaggagg tcggcgaccc gccagatcag gttgctttcg aaatcgtcga
      121 cacggccgtc ggcataggcg agctcccaca tcatttcgac cacgcggcgg cggccatcct
      181 cgtcgagcga gcgattgatg aggctggtga agtgatagag atcgatcgcc tcgtgctcgg
      241 cctcggtcgc ctcggcgacc aactcgtccg tcgtcgcctc gtcgaggtcg aattgccgct
      301 tgatgacggc gtgcagctgg tcgcgctcgg cgtcagaaat gtcgccgtcg atggcggcgg
      361 catgcacgag cagcgccgcc gccgcgaggc gatagtcgtt gtgctcgaag cggatcggat
      421 gcgcgccgtc atccgatgcc tcggacagaa atttgcgaaa gctatcgaac atccgccgtg
      481 agccgttgga tggcgccacg ttgcacgccg cctcggcaaa gtccaggggc gcgctgtcat
      541 cggcgccgcg gatctgcgct tgaaccgcgc gctcggctga tgtcttgaaa ctgcacctgc
      601 aggacgcggg actgagccta tggaatgcct gcgacattcg tgcggatttt gtaaacgctg
      661 ctgcgcgccg cgatatagag cgtcttataa tccggatcgc cgaacgccaa attggcgacc
      721 agttccggca cctggatatg acccagaacc gcgccctgcg gcgaaataat ccagatgccg
      781 ccgggtccgg actcccagat attccccttg gtgtcgacct tcatgccatc ggtgatgccg
      841 ggccgcggat cgctgttgag atcgatcaac agccggctgt tggtgagcat gccgtcggcc
      901 tggacgtcat aggcgcgaac gaagcgttcg cggctgccgt tggcatagag gattttctcg
      961 tcgggcgaga aggcgaggcc gttcgcatgg ggaatgtccc tgatcaccgc ggtcgtcttg
     1021 ccgtccttcc accggaagac acccaagaag gcgatgccgc gccgtggatc ctgatcgcgc
     1081 agccgtaatc cgccggtatc gtccgtgaag tagatcgcac cgtccttctt cacgacaaga
     1141 tcattggtgc cgttgaactt cttgccgtcg agattatcgg tcagcgtgac ccgcttgccg
     1201 ttcttctcga tcctgtcgat gccgcggccc gcccaagtgg cgatcaccag gcgaccctcg
     1261 cggtcgagcg taagcccgtt cgacccgatc atggcaaatt cctcgtagcg cggatcgtcc
     1321 ttgctgcggc cattggtctg catgaagccg acgcgccaga tgtcgaaacc ggtatagccg
     1381 ctacgatcca ggtagagcga cttctccttg ccatcggtcg acagtttgta gatcacgttg
     1441 gcgggcatat cgctcagcag cagatatccg ctctttcctt tcgcgaccca caccagtccc
     1501 tctgtgaatc catagccagt actga
//
LOCUS       DATLJT010000920         5081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_291605,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000920 DATLJT010000000
VERSION     DATLJT010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291605"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..2965
                     /gene="leuS"
                     /locus_tag="VM910_21075"
     CDS             341..2965
                     /gene="leuS"
                     /locus_tag="VM910_21075"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVI65054.1"
                     /translation="MISERYNAREAEPRWQRIWDEKAIFSSKNDDSRPKYYVLEMFPY
                     PSGRIHIGHVRNYTLGDVLARFMRAKGFNVLHPMGWDAFGLPAENAAIERKVAPKAWT
                     YDNIAAMKKQLRSIGLSLDWSREFATCDPSYYKHQQKMFLDFMRAGLVEREKRKVNWD
                     PVDMTVLANEQVIDGRGWRSGAVVEQREMNQWVFKITKYSQELLDALDTLDRWPDKVR
                     LMQRNWIGRSEGLLIRFALDPATTPAGETELKIFTTRPDTLFGAKFMAISADHPLAIA
                     AAAKDPRLAAFIAEIKRIGTAQEAIDTAEKQGFDTGIRAIHPFDPSWKLPVYVANFVL
                     MEYGTGAIFGCPGHDQRDLDFVNKYNLGVTPVVCPEGQDPKTFVITDTAYDGDGRMIN
                     SRFLDGMTIPQAKEEVAKRLETEMRGNAPVGERQVNFRLRDWGISRQRYWGCPIPVIH
                     CEKCDVVPVPDEDLPVRLPDDATFDRPGNALDHHPTWKHVTCPKCGGKALRETDTMDT
                     FVDSSWYFARFTDPWNEASPTTPAVVDRMMPVDQYIGGVEHAILHLLYSRFFTRAMKA
                     TGHIDMDEPFAGMFTQGMVVHETYQKADGSYVTPAEVKVDVVGSERRASLIDTGEVVA
                     IGPIEKMSKSKKNTVDPDDIIATYGADVARWFMLSDSPPDRDVIWSDERVQGAARFVQ
                     RLWRLINEAAEAADAAPAARPATFGDDALGLRKAAHGALNKVSGGIGRLHFNVCLAHI
                     REFANALADVLAKDSRPTADLAWAIKEAGLILAQLVAPMMPHLAEECWQVLGQGGLVS
                     ETSWPQIERDLLVEDTVTLVVQVNGKKRGEVTVASSAENPEIESAVLALDAVKLALGG
                     KTVRKVIVVPKRIVNVVG"
     gene            2952..3497
                     /gene="lptE"
                     /locus_tag="VM910_21080"
     CDS             2952..3497
                     /gene="lptE"
                     /locus_tag="VM910_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HVI65055.1"
                     /translation="MSLASTAIRLVAVVVLGALTAGCFYPMYAEHTDGTPDLREKLMG
                     VEIPPVDKPNGSREARIGVEIRNALAFKLYGNATGLPPTYRLVLRFATTRSSLMIDPN
                     TALPTSENYGIDAQFNLVDIATNKSVMTGTTFSRVTYDMPGSYQRFSRSRAYRDAEDR
                     AAQEIAENIGTRLASFFSTGT"
     gene            3544..4572
                     /gene="holA"
                     /locus_tag="VM910_21085"
     CDS             3544..4572
                     /gene="holA"
                     /locus_tag="VM910_21085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HVI65056.1"
                     /translation="MVALRGRDIDAFLARPDPGRPIILLYGPDAGLVRERADLLLASA
                     VDDPDDPFSLVKLDGDELSAEPSRLVDEAMTVPLFGGRRAIRIRAGSRNFAGGIDTLA
                     EMTLKDCRIVIEAGDLRPESPLRKACERAKTAVAIGCYPDTERDLAKLIEDELRIANL
                     RIAPDARASLMALLGGDRQASRNELRKLTLFALGEGEVTLDHVMAVVADASELKLDPI
                     VDGAFSGKPELVESEFMKAMVAGTYPGVIISSAQRQAAWLHKSALAVAEGTPVATLLE
                     SGYPRLHFSRKPMVETALRNFSAGRLVAIIDQLASAALDARKQPALAAVIAQRALLSI
                     AVNAKRKS"
     gene            complement(4588..>5081)
                     /locus_tag="VM910_21090"
     CDS             complement(4588..>5081)
                     /locus_tag="VM910_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVI65057.1"
                     /translation="DLNPLEEAQGYHALADEFKRSQDDIARIVGKSRSHVANTMRLTK
                     LPVEVQALIASGELSAGHARALIGVPDPLAAARKIVAGGLNVRQAEELAHVEGVPVRK
                     PQKPRGGKTKDADTLALEKRVSDALGLSVTIHHRDPGGTVQVSYRTLEQLDEVMRRLS
                     KGS"
BASE COUNT          921 a         1608 c         1626 g          926 t
ORIGIN      
        1 ccagacgttg aaattgttcg gtgtcggggg tacccgctgc gccgtaatgg agcgcatcgc
       61 cttatcggcg acgtcagtcg cgaactcgaa atccaggtct tcgaatgcgt cggccaaaat
      121 cttgcgcgcc cgcttccatc caggggggtc tcatttcctt tgcaggcgga taaggtctca
      181 cgaattggtt aaggcgtttg aaatccgacc ccgagcgagg tcagggcatg gccgcagggc
      241 tctctggcgg gccagccggt tgaccccccg cgcgccgtct tttatggcct aattcacgcc
      301 ccggccggac gccgaatcgg cgaaaagtta agcggattgc atgatctccg aacgctataa
      361 cgctcgcgaa gccgagccgc gctggcagcg catctgggac gaaaaggcga tcttttcgtc
      421 caaaaacgat gattcgcggc cgaaatatta tgtgctggag atgttcccct atccctccgg
      481 gcgcatccat atcgggcatg tccggaatta cacgctcggc gacgtgctgg cgcgtttcat
      541 gcgggcgaag ggtttcaacg tgctgcaccc gatgggctgg gacgccttcg ggctgcctgc
      601 ggagaatgcc gcgatcgaac gcaaggtcgc gcccaaggcc tggacctacg acaacatcgc
      661 cgcgatgaaa aaacagctcc gctcgatcgg actgtcgctc gactggtcac gggagttcgc
      721 gacctgcgac ccctcctact acaagcacca gcagaagatg ttcctggact tcatgcgcgc
      781 cggtctcgtc gagcgcgaga agcgcaaggt gaattgggac ccggtcgaca tgaccgtgct
      841 tgccaacgag caggtgatcg acgggcgcgg ctggcgctcg ggggccgtcg ttgaacaacg
      901 ggaaatgaat cagtgggtct tcaagatcac gaaatattcg caggagcttc tggacgcgct
      961 ggatacgctc gaccgctggc ctgacaaggt gcggctgatg caacgcaact ggatcggccg
     1021 ctcggagggc ctgctgatcc gcttcgcgct ggatcctgcg acgacgcccg cgggcgaaac
     1081 cgagctgaag attttcacga cgcggcccga cacgctgttc ggcgcgaaat tcatggcgat
     1141 ctcggccgac catccgctgg cgatcgccgc cgccgcaaag gatcccaggc tcgcggcgtt
     1201 catcgccgag atcaagcgca tcggcacagc gcaggaggcc atcgacaccg ccgagaagca
     1261 gggcttcgac accggcattc gcgccatcca cccgttcgat ccgagctgga agctgccggt
     1321 ctatgtcgcc aatttcgtgc tgatggaata cgggaccggc gcgatcttcg gctgccccgg
     1381 tcatgaccag cgggacctcg atttcgtcaa caaatacaat ctcggcgtca cgccggtggt
     1441 gtgcccggaa ggccaggatc cgaagacctt cgtcatcacc gataccgcct atgacggcga
     1501 cggccgcatg atcaattcgc gcttcctcga cggtatgacc atcccgcaag ccaaggaaga
     1561 ggtcgcaaag cgtcttgaaa ccgagatgcg cggcaatgcg cccgtcggcg agcggcaggt
     1621 gaacttccgc ctgcgcgact ggggtatttc gcgccagcgc tattggggct gcccgattcc
     1681 ggttatccac tgcgaaaaat gcgacgtggt gccggtgccg gacgaggacc tgccggtcag
     1741 gcttccggac gacgcgacct tcgacaggcc cggcaacgcg ctcgatcacc atccgacctg
     1801 gaagcatgtc acctgtccga agtgcggcgg aaaggcgctg cgcgagaccg acaccatgga
     1861 cactttcgtc gactcgtcct ggtatttcgc gcgcttcacc gatccctgga acgaggcatc
     1921 gccgacgacg cccgcggttg tcgaccggat gatgcccgtc gaccaatata tcggcggcgt
     1981 cgagcatgcg atcctgcatc tgctgtatag ccgtttcttc acccgcgcca tgaaggcgac
     2041 cggtcacatc gacatggacg agccgttcgc cggcatgttc actcaaggca tggtggtgca
     2101 cgagacctat cagaaggccg acggctccta cgtgacgccg gccgaggtga aggtcgatgt
     2161 ggtcggcagc gagcggcgtg ccagtctgat cgataccggc gaggttgtcg cgatcggccc
     2221 gatcgagaag atgtcgaaat ccaagaagaa cacggtcgac ccggacgaca tcatcgcaac
     2281 ctatggtgcc gacgttgccc gctggttcat gctttcggac tcgccgcccg accgcgacgt
     2341 gatctggagc gatgagcggg tgcagggtgc tgcgcggttc gtgcagcggc tgtggcggct
     2401 gatcaacgag gccgcagagg ccgccgacgc cgcaccggct gcgcggccgg cgacattcgg
     2461 cgacgatgcc ttgggtttgc gcaaggcggc acacggggcg ctgaacaagg tgtccggcgg
     2521 gatcgggcgg ctgcatttca acgtctgcct cgcccatatc cgcgaattcg ccaatgcgct
     2581 ggccgatgtg ctcgcgaaag acagtcggcc aacggccgac ctcgcatggg cgatcaagga
     2641 ggccggcctt atcctggcgc agctcgttgc ccccatgatg ccgcatctgg cggaagaatg
     2701 ctggcaggtg ctggggcagg gggggctggt ttcggagacc agttggccgc aaatcgaacg
     2761 cgatttgctg gttgaagaca cggttaccct ggtggtccag gtcaacggca agaagcgcgg
     2821 tgaggtcacg gtggcaagct ccgccgaaaa ccctgaaatt gagagtgccg ttttggcgct
     2881 cgatgcggta aaattggcct tgggcggtaa gaccgtccgc aaagtgatcg tggttcccaa
     2941 gaggatcgtg aatgtcgttg gctagcaccg ccatcaggct cgtcgccgtg gtcgtcctgg
     3001 gggcgctgac ggccggctgc ttctacccga tgtatgccga gcacaccgac ggcacgccgg
     3061 acctgcgcga aaagctgatg ggggtcgaga tcccgccggt cgacaagccc aacggctcgc
     3121 gcgaggcccg catcggggtc gaaatccgca acgcgctcgc cttcaagctc tatggcaacg
     3181 cgaccggctt gccgccgacc taccgcctcg tgctgcgctt cgcgacgacc cgttcctccc
     3241 tgatgatcga ccccaacacg gcgttgccca ccagcgaaaa ctacggcatc gacgcgcaat
     3301 tcaacctggt cgatatcgcg accaacaagt cggtcatgac cggcaccacc ttctcccggg
     3361 taacctacga catgccgggc tcctatcagc gcttttcgcg atcgcgcgcg taccgtgacg
     3421 ccgaagatcg cgccgcccag gagattgcgg aaaacatcgg cacgcgactc gcatcgtttt
     3481 tctccaccgg cacctagtgc gtgatctcca cggcgcgaac ttcggaatga gggcactcga
     3541 aagttggtcg cgctgcgcgg aagggacatc gatgcgtttc tcgcccggcc cgatccgggt
     3601 cgtccgataa ttcttcttta tggtccagac gccggcctcg tgcgcgagcg cgccgactta
     3661 ctgctcgcct ccgcggtcga tgatcccgac gaccccttct cgctggtgaa gctcgacggc
     3721 gacgaactct ccgccgaacc ttcgcggctg gtcgatgagg cgatgacggt gccgttgttc
     3781 ggcggccgtc gtgcgatccg aatccgtgcc ggctcgcgca attttgccgg cggcatcgac
     3841 acgctggccg agatgacttt gaaggactgc cgcatcgtca tagaggccgg cgacctgcgt
     3901 ccggagtcgc cgctgcgcaa ggcctgtgag cgcgccaaga cggcggtcgc gatcggctgc
     3961 tatccggaca ccgagcgcga ccttgccaag ctgatcgagg acgaattgcg tatcgcgaat
     4021 ttgcggattg cgccggatgc gcgcgcctcg ttgatggcgc tgctcggcgg cgaccgccag
     4081 gcctcgcgca acgagctgcg caaattgacg ctgtttgccc ttggcgaggg cgaggtgacg
     4141 ctcgaccatg tcatggccgt ggtcgcggac gcctccgagt tgaagctcga cccgatcgtc
     4201 gacggcgcgt tttccggaaa gccggagctg gtcgagagcg aattcatgaa agcgatggtc
     4261 gccggcacct atcccggcgt catcatttca tcggcccaac gtcaggcggc gtggctgcac
     4321 aagtctgcgc tcgcggtcgc ggaagggacg ccggtcgcga cactgctcga aagcggctat
     4381 ccgcggctgc atttctctcg caagccgatg gtggagaccg cgctccgcaa tttctccgca
     4441 ggcaggttag tcgcgattat cgatcagctc gcgagcgccg cgctcgatgc gcgcaagcag
     4501 ccggcgcttg ccgccgtcat cgctcagcgc gccctgctgt cgattgcggt gaatgcgaag
     4561 cggaagagtt agcttttgga gcgcgcttca ggagcccttc gacagccgcc gcatgacctc
     4621 gtcaagctgc tccagagtcc ggtagctgac ctgcacggtg ccgccgggat cgcgatggtg
     4681 gatggtgacg gaaagaccga gcgcatcgct gacgcgcttc tcgagcgcaa gcgtatcggc
     4741 atctttcgtc ttgccgccgc gcggcttctg cggcttgcgc accggcacac cctcgacatg
     4801 agcgagttcc tcggcctggc ggacgttgag acctcccgcg acgattttcc ttgcggcggc
     4861 cagcggatcg ggcacgccga tcagggcgcg cgcgtgcccg gccgacaatt cgcccgaggc
     4921 gatcaacgcc tgcacctcga cgggcaattt ggtcagccgc atcgtgttgg cgacatggct
     4981 gcggctcttg ccgacgatac gcgcgatgtc gtcctggctg cgtttgaact cgtcggcgag
     5041 tgcgtgatag ccctgcgcct cttccagcgg attgaggtcc t
//
LOCUS       DATLJT010000921         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_978535,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000921 DATLJT010000000
VERSION     DATLJT010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978535"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(431..1546)
                     /locus_tag="VM910_21095"
     CDS             complement(431..1546)
                     /locus_tag="VM910_21095"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HVI65058.1"
                     /translation="MIEVADVFRRFVEDYLNAHGATMPPSHRRTIADILACRTEVLGG
                     HLWRCNHCCHEVFSYHSCRNRSCPKCHTDQTKVWLAHRQPELLATHYFHVVITVPEEL
                     RDVLRSNQRDGYALLMKAAAEAIIDLARDRRFVGGTVGILAVLHTWTQQLLYHPHVHC
                     LVTGGGISEDGRDWYPARDAFLLPYQALADRVRGKFRALLASKRPDLIAPYNPAWTTS
                     WVVHIARWGQGAQAVLDYLARYVFRIAITNARLVALDHHTVTIRYKHRKSSRWRTCRM
                     EGSEFMRRFLQHVLPKGLHKVRYFGLWHPAKRAQAAQARLLLELDRTKPAPSQDNQTA
                     ERKADQPALSEPRICPCCRHGQLIYVRRLTPKNALGP"
     gene            complement(1562..2386)
                     /locus_tag="VM910_21100"
     CDS             complement(1562..2386)
                     /locus_tag="VM910_21100"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI65059.1"
                     /translation="MIEDMTLSGLAAGTQATYIDAVRKLAAHYRCSPDQLTEEEVRAY
                     ILHLRESGAARGTFKANHYGIQYFYRQTLNRDWTLFSKKKIRLPKQKRLPHAIADAEV
                     RKLLALTTNPIHRTCFALMYACGLRTGEAVSLEIPAIDGRKHLLTIIGKGNKERLVPL
                     PDPMLWSLRRLWQLHKNPRWLFPNRAGDHTIGRGTLNRTFSLVAQAAGIRSVTPHSLR
                     HAYATRLLENGVNVRVVQLLLGHSQIATTALYLHLTEPTRTSLTNLLDKLMVDLGP"
     gene            complement(2535..3395)
                     /locus_tag="VM910_21105"
                     /pseudo
     CDS             complement(2535..3395)
                     /locus_tag="VM910_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          760 a         1018 c         1227 g          707 t
ORIGIN      
        1 gcatgttgct ggtggtttag gcgccgccac gctgttgaag caacgtggcg gcgtcgcctc
       61 attagcacaa cgaaaaaccc gcacaagccg gtgatccaaa acgaattaag ggtttctgcg
      121 gcgctttgcc gacgcagaaa ccgcgttgga ccgacgcggg ggttgggcac cccgcccggt
      181 caacgagtat ggaatttgta atttgagggc tgagtgagcc ggagaatagc ggacgatgcg
      241 aggagagttt ttagcggtcg gagcaagatg ggttatgagg gagattttct ccacggcgtg
      301 agtggttgca cgagatcttg gcgggcgatt gacaatgacg cgtgatctgg cgaatcggca
      361 gcagcagagt ggtgcggcat gccgggcacg cgaacagaat ccatgattgt ggcggcgcgc
      421 aggtgaggga tcatgggcca agcgcgttct ttggtgtcag ccggcgaacg tagataagtt
      481 gcccgtgtcg gcaacagggg caaatccttg gttcgcttag agcgggttgg tcggctttgc
      541 gctcggcggt ttgattgtcc tgggatggtg ctggcttggt gcgatcgagc tccaggagga
      601 gacgcgcttg agcggcctgc gcacgctttg cgggatgcca gagaccgaaa tagcggacct
      661 tgtggaggcc cttgggaagg acgtgctgga ggaagcggcg catgaactcg ctgccttcca
      721 tgcgacaggt gcgccaacgc gacgatttac gatgcttgta tcggatggtg acggtgtgat
      781 ggtcgagggc gacgaggcga gcattggtga tggcgatgcg aaagacgtag cgcgcgaggt
      841 aatcgagcac ggcctgcgcg ccctggcccc aacgggcgat gtgaacgacc caggatgtgg
      901 tccaggcggg attgtagggg gcgatcagat cgggacgttt gcttgccagc aaggcacgga
      961 acttgccgcg cacccgatca gcgagggcct ggtaaggcag gagaaaagca tcgcgggctg
     1021 ggtaccagtc gcggccatcc tcggaaatgc caccgccggt cacgaggcaa tggacgtggg
     1081 ggtggtagag aagttgctgt gtccaggtgt ggaggacggc gaggataccg acggtgccgc
     1141 cgacgaagcg gcggtcgcgg gcgagatcaa tgatggcctc tgctgcggcc ttcatcaaca
     1201 gagcgtaacc atcacgctga ttggagcgca gaacgtcgcg aagttcctcg ggcacggtga
     1261 tcacaacatg gaagtaatgg gtcgccagca attcgggctg acgatgtgcg agccacacct
     1321 tggtctggtc ggtgtgacac ttgggacagc tgcgattcct gcacgagtga taagaaaaca
     1381 cctcatggca gcaatgattg caccgccaga gatgcccccc gagcacctct gtacggcaag
     1441 cgagaatgtc ggcgatggtc cggcgatgcg acggcggcat cgtcgcgcca tgcgcgttca
     1501 gatagtcctc cacgaagcgg cggaagacat ccgcaacttc gatcatggcg gctgaccgag
     1561 gtcaggggcc gaggtcaacc atgagcttgt cgaggagatt ggtgagcgag gtgcgggttg
     1621 gctcggtcag atgcaggtag agggccgtgg tggcaatttg gctgtgcccg agcagcagtt
     1681 gcacgactct gacattaacc ccgttttcga ggagcctggt cgcataggcg tggcgaagac
     1741 tatgaggcgt caccgaacgg atgccggcgg cttgggcgac gagcgaaaac gtgcggttga
     1801 gggtgccccg cccgatcgtg tggtctccgg cccgattggg gaacagccat cgcgggttct
     1861 tatgaagttg ccaaaggcgc cgcaggctcc agagcatcgg atcaggcagt gggaccaggc
     1921 gctccttgtt acccttgccg ataattgtga ggaggtgctt gcgaccgtct attgccggaa
     1981 tttccaggct caccgcctcg ccggttcgca gaccgcaggc gtacatgagc gcgaagcagg
     2041 tccgatggat cgggtttgtg gtcagggcga gcagcttgcg cacctcggca tcggcaatcg
     2101 catggggtaa acgcttctgc ttcggcaggc ggatcttttt ttttgagaac agggtccaat
     2161 cacgattgag ggtttgccga tagaaatact ggatgccgta atggttggcc ttgaaggtcc
     2221 cacgcgccgc tccgctctcc cgcaggtgca ggatataggc gcgcacctcc tcctcagtga
     2281 gttgatctgg tgagcaccta taatgtgccg ccagtttacg caccgcgtcg atgtaagtgg
     2341 cttgggtacc agccgccaac cccgacaaag tcatgtcttc gatcatccgc atccgtaatg
     2401 ctgacatggt aggtctcctt tcggaatggt gatcgggcgc aacaggcccg atcccaccaa
     2461 gaagacccaa caattcggcg cggcggcggc ctcatactcc cctcggcgaa gccgtcggtc
     2521 caacatggat agaagcgacg attgtagcga aacacgaatt cgttgagata cgtgtcaacg
     2581 tgtttgcgac gtaggccatg atatgtgccg agggcccatc gcttgagcag cgagaaagcc
     2641 cgatggatcc acggtaaaat gacgtgaccg gccatggcgc cgacaacgcg gggatcgtgc
     2701 cggtagccgc ttaagcctgg atatgacgca tggccatcgg tcagcagcgt ggttcccggc
     2761 ttcacgttgg ctctcacgaa ggccgcgatc gacgcggttg aattatcggc gatcacggcg
     2821 aagcggaggc ggccggaacg cgtatcgaga tatttggcgc gcttgcggcg cggtttggct
     2881 tgattgctgc cgcgatcgat gacctcgacg gcgccaatga cgaggattat cccgtcgttg
     2941 ccgggttcga agaatgcatt gtccccgcgg aaagggattt ccgcctgatc gacttcaacg
     3001 accccttcga gcggttcccg atttggatcg accatcgatc gccgcaactt ttgcgtcagt
     3061 agccacgcgg tcttgtaggt gacgccgagt tggtcctcca attgacgcgc cgacataccg
     3121 ttggaatggg ttgtcatgag atgcgcagcc cagaaccatg ctgtcagcgg cagcttggag
     3181 cgatgcatcg tcgtccccgc cgtgactgag gtctgccggc cgcaatcgag acattcatgc
     3241 aaacgcggtc ggcttttcag cccaaccgcg cggcgcccgc cacagccggg acacacaaaa
     3301 ccatccggcc agcgccactt gaataaaaaa gccgcgcagc tctcctcgtc gggaaacgac
     3361 ttctggaact ccgcgagcga ccgagccctc gccatcggtg tccgcccgcg cgaccgcgcc
     3421 aatccgatcg ctcagcacac tacatctggg gaaacctgag tcaacgggat aagccgctgg
     3481 cagagtgccc agttaaccgg ctgcgccgcg ccgaacgaac attctggcct tggccaccct
     3541 tgcgtaaacg ttacgtgttt cgcacctaga tcgcgcgtta cagatggcgc cacggtccta
     3601 ggaagcccgt agagcgtggt ggcaagcaac gagagcacag cccagccaca gccatgaaaa
     3661 ggcgcatagg actactctcc ttcggcgcga gggcgcactg gtatgtgact ac
//
LOCUS       DATLJT010000922         1622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3737506,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000922 DATLJT010000000
VERSION     DATLJT010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3737506"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1622)
                     /locus_tag="VM910_21110"
     CDS             complement(<1..>1622)
                     /locus_tag="VM910_21110"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI65060.1"
                     /translation="QTATAEVLNIISRSTFDAQPVFETIIKNAARLCQSVLSAIYRSD
                     GELVHLAAHDQFSPESVAAVRAAYPVPITSSNLIAVAVRERRVVHIPDVLVSGGYTEL
                     QRTSGYRGILIVPMMRDDVAIGAIAVMQIEPRLFPDTQVELLKTFASQAVLAIDNVRM
                     FEEVQARTREVQESLEYQTAISDVLNVISRSPNELQPVIDAILQTAARLCEAEYALFW
                     RLQDGAYHLAGASNADAAFVKHCAEHPIRPGRGSVAGRTALEQKTVHLPDCLADPEYT
                     LLDFQEVGGHRSMLGVPLLRDGVTIAIIVLLRNAVRPFTDKQIDLVTTFANQALIAIE
                     NVRLFDEVQARTRDLSESLEQQTATAEVLQVISSSPGEPQPVFEAMLANATRLCEAKF
                     GTLYTYDGNGFHAAAFHNAPPAYVEARTREPVLRPPPDTPVGRIAATRQLVQIADIKT
                     IPSYIEGHRFVRAAVDLAGYRTVLAVPMLKEAELVGAIVIYRQEVRLFTQKQIELVTN
                     FATQAVIAIENTRLLNELRESLEQQTATSEVL"
BASE COUNT          298 a          499 c          518 g          307 t
ORIGIN      
        1 ccagcacctc cgaagtcgct gtctgctgct ccagggactc gcgcagctca ttgagtaggc
       61 gcgtgttctc gatggcgatg acggcttggg tggcaaaatt cgtaacaagc tcaatctgct
      121 tctgggtgaa caggcgtacc tcctgacggt agatgacaat cgcaccgacc agctcggctt
      181 ccttgagcat cgggaccgcc agcaccgtcc gatagccggc gaggtcgaca gccgcgcgaa
      241 caaaccgatg gccctcgatg taggacggta tggtcttgat gtcggcgatt tggacgagct
      301 gtcttgtggc ggcgatgcgg cccaccggtg tgtccggcgg cgggcgtagc accggctcgc
      361 gcgtgcgtgc ctcgacatat gcgggcgggg cgttgtgaaa cgctgcggcg tgaaaaccgt
      421 tgccatcata cgtgtagaga gtgccgaact tcgcctcgca gaggcgcgtc gcgttcgcca
      481 gcatggcctc gaacacgggc tggggctcgc ccggcgaact ggagatgacc tgcagcacct
      541 ctgcggtcgc cgtctgctgc tccagcgatt cggagagatc gcgcgtacgc gcctgcacct
      601 cgtcgaacag ccgcacgttc tcgatcgcga tcaacgcttg gttggcgaag gtcgtcacca
      661 ggtcgatctg cttgtccgtg aacggtctca cggcattgcg caggagtacg atgatggcga
      721 tcgtcacgcc gtcgcgcagc agcggaaccc cgagcatact gcgatggcca ccgacctcct
      781 gaaagtcaag aagggtgtat tcaggatcag ccagacaatc cggcaagtga acggttttct
      841 gctccagcgc cgtccggccg gcgactgaac cgcgtccggg acgaatcggg tgttccgcgc
      901 agtgcttcac aaatgcggca tccgcattgc tcgcacccgc caggtggtaa gcgccgtcct
      961 gcagcctcca aaagagagcg tactccgcct cgcacaagcg cgccgctgtt tgcaggattg
     1021 catcaatgac tggttgcagc tcgttgggcg agcgagagat gacgttcagc acgtcgctga
     1081 tcgccgtctg atattcgagc gattcctgca cctcgcgtgt gcgcgcctgc acctcctcga
     1141 acatccgcac gttatcaatg gcgagcaccg cttggctcgc gaacgtcttg agcagctcta
     1201 cctgcgtgtc gggaaacagt cgcggctcga tctgcatcac ggcgatggcg ccgatggcga
     1261 catcgtcgcg catcatcggc acgatgagga tgccgcggta gccggatgtc cgctgcagct
     1321 cggtatagcc gcccgagacg agcacgtccg gaatgtgcac gacgcgtcgc tcgcggacag
     1381 cgaccgcgat caagttgctg ctggtgatcg gaacgggata agcggcccgc accgctgcca
     1441 ccgattccgg cgagaactgg tcgtgcgccg caaggtggac caactctccg tcactgcgat
     1501 agatcgcgct gagcacgctt tgacagagcc gagcggcgtt tttgatgatc gtttcgaaca
     1561 cgggctgcgc atcgaaggtc gagcggctga tgatgttgag aacctccgcc gtggcagtct
     1621 gc
//
LOCUS       DATLJT010000923         1923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_61657,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000923 DATLJT010000000
VERSION     DATLJT010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61657"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1021)
                     /locus_tag="VM910_21115"
     CDS             complement(join(261..682,682..1021))
                     /locus_tag="VM910_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611663.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVI65061.1"
                     /translation="MRAGLFWLNDGQWARIEPHLPTGLTGPDRDDDRRIISGIIHMLQ
                     SGARWRDCPREYGPYTTVYNRFNRWAKRGRWCAIFEALAKPGKDAVALSLDSTSIKAH
                     RCASGGKGGSTNQAIGRSRGGRTTKIHALSDPLCRPVVLHLTPGHDADIAAAPDVLAL
                     APPMSALLADKGYDGDNLRGDIVRRGAKPVIPNKSNRVVIHRFNKRAYRGRNVIERCF
                     CRLKDFRRIATRYDKLARNFLAAIHLAALVAYWLN"
     gene            complement(1245..>1923)
                     /locus_tag="VM910_21120"
     CDS             complement(1245..>1923)
                     /locus_tag="VM910_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HVI65062.1"
                     /translation="KNVEFATKPQIARRMITRAIAAKVPFSFVAADTVYGTGMIEALL
                     RNAGKGYVLGVAANRVFHSWGKPQLVSGTAATIAQSLPKTAWRRLSSGEGTKGPRLHD
                     WAYVELADLEAGTLAGEWTRGLLIRRNIASDDLAFFSTWCPKGTPMTKLVAVEGHRWA
                     IEDSFETAKNEFGLDHNETRSWHGWHRHVSLVMLAFAMMAVIRHQANAESAPKKTPPR
                     RHTKHRS"
BASE COUNT          378 a          558 c          602 g          385 t
ORIGIN      
        1 ggtgggcagc atcgctggca cgcagctcac ctggccgcgg tttctgcagc ggcgcgccga
       61 atacgaagcg tggaccgatc cccggctggt cctggatacg tcgcgcacat cgccagcgca
      121 gttgcttacg caagcgctga attacacgcg ataggctgca aaatggcgcc cctacgatct
      181 ctcaggttgt ccgatttacc cgcccgagta tatcccaatc tacgacgagc acgtaaatcg
      241 atctgtacta gggtccagac tcaatttagc caatatgcga cgagagcggc gagatgaata
      301 gcggccaaga aatttcgggc gagcttgtca tatcgggtgg cgatgcggcg gaagtccttg
      361 agcctgcaaa agcagcgctc gatgacattt cggcctctgt aagcgcgttt gttgaagcga
      421 tggatgacaa cgcgattgga tttgttgggg atgacgggct tggcgccgcg acggacgatg
      481 tcaccgcgaa ggttgtcgcc gtcatagcct ttgtcggcga ggagcgcgct catgggcggc
      541 gcgagcgcca gaacatctgg cgccgccgcg atatcggcat cgtggcctgg ggtcaggtgc
      601 aggacgaccg gccggcagag cggatcgctc agcgcatgga tttttgtcgt gcggcctccg
      661 cgcgagcggc cgattgcctg attgtgctcc ccccttttcc gccggaggca caccggtgag
      721 ctttaatcga ggtcgagtcg agcgacagcg cgacggcatc tttgccgggc ttggccagcg
      781 cttcaaagat cgcgcaccat cgtccacgct tggcccagcg gttgaagcga ttgtagacgg
      841 tcgtgtaagg cccgtattca cgcgggcaat cgcgccatcg cgctcccgac tgcagcatgt
      901 gaatgatccc gctgatgatg cgccggtcgt cgtcgcgatc cggccccgtc agtcccgtcg
      961 gcagatgagg ctcgatacgc gcccattgcc cgtcattcag ccaaaacaaa cccgcgcgca
     1021 ttctcccgcc cccgaatcaa aaactcgcgg gcaaaggaat cacatcgcgt tattcaggta
     1081 cagaccctag cattacagtt gcattttccg cttgaggtga gcgcggcgag cgtgggcctg
     1141 gtgggctcgc cgccagagcg accatgcgag aacgcggtcg atcgagatgc gccgttgagc
     1201 gagcttgata gcgatgcgac ggatttcctg gatcgaccag cggatcacga acgatgtttg
     1261 gtgtgccggc gtggcggtgt ttttttgggt gctgattcag cattggcctg atggcggatg
     1321 acagccatca tggcgaaggc gagcatgacg agggagacgt ggcggtgcca gccatgccag
     1381 gagcgggtct cgttatgatc gaggccgaat tcattcttgg cggtttcaaa actatcctcg
     1441 atagcccagc gatgaccttc caccgctacc agctttgtca tcggtgtgcc cttgggacac
     1501 caagtggaga agaaggccag atcatcactg gcgatgttgc ggcggatcaa gaggcctcgt
     1561 gtccattccc cggcaagagt gccagcctcg agatctgcca gttcgacgta ggcccagtca
     1621 tgcagacgcg gccccttggt tccttcgccg gacgacaggc gtcgccaagc agtcttggga
     1681 aggctctgtg caatcgtggc ggcggtgccg ctaacaagct gcggcttgcc ccaggaatgg
     1741 aacacacgat tggcggcaac gcccagaaca tagcccttgc ctgcattgcg aagcagcgct
     1801 tcgatcattc cggtgccata cacggtatcc gccgcgacga aggagaacgg caccttcgcg
     1861 gcgatcgcgc gtgtaatcat ccgacgagcg atctgaggtt tggtagcaaa ttctacattt
     1921 ttc
//
LOCUS       DATLJT010000924         1950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2128755,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000924 DATLJT010000000
VERSION     DATLJT010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128755"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VM910_21125"
     CDS             <1..774
                     /locus_tag="VM910_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65063.1"
                     /translation="ARERRLPLQIHAAQSVVEFQEIMRRHGRTPIGWLRQIGVLGDNA
                     IIGHAIFLDHHSWLHWPTRNDLADLADTGTTVAHCPTVFSRRGILLEDFGRYRAAGIN
                     LGIGTDTFPHNFVEELRTAAVLARIAAGNTHAVTAADIFTAATIGGARALGRDDLGRI
                     TPGAKADFVMVDIAHPAMQPLYDPVRSMVYAAGERAVRHVFVGGQQVVRDGKALAFDY
                     ADASARLAMAQRRAIEKVPRLDWAGRSAAEIMPPTFRSE"
BASE COUNT          332 a          630 c          611 g          377 t
ORIGIN      
        1 gcccgcgagc gccggctgcc gttgcagatt catgccgcgc agagtgtggt cgagtttcaa
       61 gagatcatgc ggcggcacgg caggacgccg atcggctggc tgcgccagat cggcgtgctc
      121 ggcgacaacg caatcatcgg ccatgcgatt tttctcgacc atcattcctg gctgcactgg
      181 cccacccgaa acgatcttgc cgatcttgcc gacaccggca ctaccgtggc ccactgcccg
      241 accgtgtttt cgcgacgcgg catattgctg gaggatttcg gccgatatcg cgccgcgggg
      301 atcaatctcg gcatcggcac agacaccttt ccgcacaatt tcgtcgagga gctgcgcact
      361 gcggcggtcc tggcgcgcat agccgcgggc aatacacatg ccgtgaccgc ggcggacatc
      421 ttcaccgccg ccacaatcgg cggcgcccgt gcgctgggcc gcgacgatct ggggcggatc
      481 acgcccggcg ccaaggccga cttcgtgatg gtcgatatcg cgcatccggc gatgcagccg
      541 ctttatgatc cggtccgcag catggtctat gccgccggcg agcgcgcggt gcggcatgtt
      601 tttgtcggcg ggcagcaagt cgtccgcgac ggcaaggcgc tcgccttcga ctatgccgat
      661 gcgagtgccc ggctcgcaat ggcacagcgc cgcgcgatcg agaaggtgcc tcgcctcgat
      721 tgggccgggc gcagtgccgc cgagatcatg ccgccgacct ttcgctcgga gtaagcttca
      781 gccgattttc acggcagagg tggtgcaacg cgctatgggt cgcggctgtg tgagaatctc
      841 ccgggggaga ccgcttcggg ggttgctgtg aagcgtttgg cgccttctgg aggctcggtg
      901 acccggcgtg gatatcattg ccggggccag cctggctcgg cgcacggtaa acttgccgtt
      961 gtcggtcgtc aaaacccggc tgcggctccc gggcctttgc ggctctaagc tccggagcgg
     1021 tgtaaaaaat tgctcgacct aaatccagga accggaattt cgaatccatc tccctccagc
     1081 aaggggtctg caaactttcg gttcctggtc gtgccaacct gcggtggcag ataccatgcg
     1141 ccctccatcc cttgccgacg cctcggccgc ttagtgtccg ctaagctgtt agtggacact
     1201 tgttctgttt gctccgccat ccgccgatca tcgcgccaat cggcgtttag cggcaggcgc
     1261 tcctcaccga gcgcttacgg catcatgggc atgcgactcc gccgagcggt tgatgcggcg
     1321 cggcgtcggg accatcggcc tgagcccggc gccgcttcgt tcctcgtcat tccgttatcg
     1381 gtgaggcagc aaatgcgtcg gtaacgccat tggcgcgagg gcgatacagc tgcgaaaagc
     1441 aagcctccgc gtccaccgcg cgtagaaccc gaccccgtct tgacggctgc gacctcggcc
     1501 ccgcaaggtc cgccctcgtc ttgctgcacg gtattgggac gacgccattg atatctcacc
     1561 cgcgggcatc gcatcgggcc gggccgcgat cgagaagcgc ttcactgaag agttcaacgc
     1621 aaacgactta aagggcttcg ccgaaacgat cgatgcggca cacgtgtcgg gcaactcggg
     1681 ctggttcgtc ggccattgga gtgcgacttt tctatcgcgt gctagcggcg agcgccgtcc
     1741 cattaaaggt tacgtcgccg ctgtgctcga agggcggaat ggcgagtgga aggcacgcat
     1801 ccacatcatc cgcggtcccg gccgacatcc gcgatttgat ccagaaaatc agccatgaca
     1861 acccgctgtg gggcgcgcca cgcattcatg gcgagctgct gaaacttggt atcgatatcg
     1921 cccagtcgac tgtcgccaag tacatctcac
//
LOCUS       DATLJT010000925         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3738010,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000925 DATLJT010000000
VERSION     DATLJT010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738010"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1691
                     /locus_tag="VM910_21130"
     CDS             315..1691
                     /locus_tag="VM910_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HVI65064.1"
                     /translation="MSDDTPLPFDLPSVCRKKLTVDFNGGNQSSNGGVLLLREAERSV
                     GVCRLLANTMTDRRDPNRIRHEVFEMLMARVSAIACGYEDANDLDRLRHDPLMKLAVG
                     RCPEGGEALASQSTISRLENAPRKTEAARLCAALIDLFGTTVKPGKLEILDIDDTFCA
                     AHGGQQLAFWNAHHNERGFAPMHIYHVASGTPVVAILRAARTPKGTEVRTVIKHVTKR
                     LRKHWPNTRIVWRGDGHYGRVEEMERAESDGTDYIFGLAGNPVLDVLMAETADNLCFH
                     HAGSNQTKLRTYASFFYQASSWDRARKVVARLECSLQPDGEEGTATGMRQEVDIRYVV
                     TSLEGAAQHLYEDVYCQRGQMENLIKLHKAQLSSDRMSCHSATANQVRLVLHTAAFWL
                     MHGVRAAIPQTNPLANAEFATIRERLIKLGARVIEHIARIRIHLPSSCPERALFRTVA
                     LSLMPCGP"
     gene            1977..>2293
                     /locus_tag="VM910_21135"
     CDS             1977..>2293
                     /locus_tag="VM910_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVI65065.1"
                     /translation="MVSESNLHTAEMEARETFSLIGSDKVEGTPVYRSNGDKIGRIER
                     LMIYKVGGKVAYAIMSFGGSMGIGEDYYPLPWFAVGTAVASRPPHRSVQAGFPHTAPT
                     LSV"
BASE COUNT          461 a          691 c          683 g          458 t
ORIGIN      
        1 ataggcgatg agattaagca gcaagttggc ggtgacgctg gcaatccagc ggtgcttggt
       61 cttgcgcaag gtgccgtgtt ggttgccata gccgaaaatg cattcgatcg acgcccggcg
      121 gctttgcgac atgccgtagc ctcgtgccgc gtggtgcggc gtcgatcgcg ctcctgcccg
      181 cttgccggtc ttagcccgga aaattcatca ggcctgagaa acttgcggcg actgtagcgc
      241 ttagaccgcg attcacgtag caaatcgttg tccaaacaaa gtgccttgcg tatcagccct
      301 gcgccacagc cgccatgtcc gacgatacgc cgcttccttt cgaccttcca tctgtctgcc
      361 gtaagaaact cacagttgat tttaacggcg gcaaccaatc ctccaatggt ggcgttctgc
      421 tgctgcgaga ggcggagcgc tcggtcggtg tatgccggct gcttgcgaac accatgacgg
      481 atcggcgcga tccgaatcgc atccgacatg aggtgttcga aatgttgatg gcgcgggtgt
      541 ctgcgatcgc ctgcggctac gaggatgcta acgacctcga tcggctgcgc catgatcctt
      601 tgatgaagct tgcggttgga cgttgtccgg agggcggcga agcgctggcc tcgcaatcga
      661 ccatctctcg cctcgagaat gcaccgcgca agaccgaggc ggcgcggcta tgtgcagctc
      721 tcattgacct gttcggcacc accgtgaagc cgggcaagct tgagatcctc gacatcgacg
      781 acaccttctg tgcggcccat ggcggccagc agcttgcgtt ctggaacgcg catcacaatg
      841 aacgcggctt tgcgccaatg cacatctatc atgttgcgag cggcacgccg gttgtggcca
      901 tccttcgtgc ggcgcgcacc ccgaagggca cggaggtgag gaccgtcatc aagcacgtga
      961 ccaagcgcct gcggaaacat tggcccaaca cccgcatcgt gtggcggggc gacggccatt
     1021 acggtcgcgt cgaggagatg gaacgggcgg aaagcgacgg caccgactat atttttggcc
     1081 tcgccggcaa tccggtgctc gatgtcttga tggccgagac cgccgacaat ctgtgcttcc
     1141 atcacgccgg gagcaatcag accaagctgc gcacctatgc gagcttcttc tatcaggcca
     1201 gcagctggga tcgtgcccgc aaggtggtgg cgcggctgga gtgctcgttg cagccggacg
     1261 gcgaagaggg caccgcaacc ggcatgcgcc aggaggtgga catccgttat gtcgtcacct
     1321 cgctcgaagg cgccgcgcag catctatatg aggacgtcta ttgccagcgc ggacagatgg
     1381 agaatctgat caagctgcac aaggcgcagc tctcctcgga tcgcatgtcg tgtcacagcg
     1441 ccaccgccaa tcaggtgcgg ctcgtcctcc acaccgcggc gttctggctg atgcatggcg
     1501 tgcgcgccgc cattccgcag acgaacccac tggcgaacgc tgaattcgca acgatacgcg
     1561 agcgcctgat caagctcggc gcgcgggtga tcgagcacat tgcccgcatt cgcatacatc
     1621 tcccatcgag ttgccccgag cgggcgctgt tccgaaccgt tgcgctctct ctcatgccgt
     1681 gcggcccatg agcaacgggg ccacggggcc ccctacgccc gcccatttgg gccgatcaac
     1741 cctaaacgcg ttgcacggcg agtcacatta caccgatcag ctggtcggag ttcgccctcg
     1801 tctgctcaac acacgtgtga aaacccatca cgttatgcat tattgcggtt aggcgcgtag
     1861 atcgagatat gtcccaccag atcaacatcg tgacgaattg agcattccac attcagccat
     1921 ccagaactcc tagcgagggt caacgttgcc aaaggggcta aaacgaggag aaccatatgg
     1981 tctcagaatc aaacctccat acagcggaaa tggaggcgcg tgaaacattc tcgctcatcg
     2041 gcagcgataa agtcgaaggg accccggttt accggtctaa tggtgacaag attggtcgga
     2101 tcgaacgttt gatgatttac aaagtcggcg gcaaagtcgc ctacgcgatc atgagttttg
     2161 gcggttccat gggtatcggc gaggattact atccgctgcc ttggttcgcg gtagggacgg
     2221 ctgttgccag ccgccccccg cacagatccg tacaggcggg tttcccgcat acggctccta
     2281 ccttgagtgt ttg
//
LOCUS       DATLJT010000926         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_292211,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000926 DATLJT010000000
VERSION     DATLJT010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292211"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..1049
                     /locus_tag="VM910_21140"
     CDS             402..1049
                     /locus_tag="VM910_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65066.1"
                     /translation="MAILQEGRGLASEAEPLGLLTEGAAGIAVIVLAVIALAGTLSGA
                     LASIVTIVVGVGLMVQAFNSAAENSKIMAANAAPIPDPGGEVVVDGLSGITGIILGVL
                     ALVGINAPYLVASALIVFGGALFLSGAISLPLRAWQRTAASGQTQVVFYHGSAAASGV
                     EILIGIAAIVLGILSLIFVGSWVLALVGFIAVGAALLMVSATFSGTMMRLFTTTA"
     gene            1719..1899
                     /locus_tag="VM910_21145"
                     /pseudo
     CDS             1719..1899
                     /locus_tag="VM910_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1912..>2274
                     /locus_tag="VM910_21150"
     CDS             1912..>2274
                     /locus_tag="VM910_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HVI65067.1"
                     /translation="MAAKEIRFHTDARDRMLRGIDILSNAVRVTLGPKGRNVVLDKSF
                     GAPRISKDGVTVAKEIELADKFENMGAQMVREVASKTSDQAGDGTTTAIVLAQTIVRE
                     GAKAAAAGMNPMDLKRGID"
BASE COUNT          446 a          637 c          721 g          470 t
ORIGIN      
        1 ctccatcggt ggagcgcgag aacgcgagca cgggtatctg acaccggcca aactcttggc
       61 gatgacccga tcgggcctgt aaaatgagca cgccgcgtcg gcgaaggaga tcctcgcaaa
      121 cttcgagcta gcgatgaccc aagaggccat ctggccttcg aacgtgagct cctccgcgag
      181 cagcattacg ctttgcctta gaatgatccg cagatggcgc cgcatgagcg ccccgctttc
      241 cgagttcatc atgctcaagc tacccaacgt taagtttgaa tctcccttcg cacgttatgg
      301 ttggcacgct gttaacttta tgctgtataa ttcgccgatg cggcgcgagc gcccaatgtg
      361 atgcttgcgg gcactcagcc ggcaagattg ggaggactga aatggctatc cttcaggaag
      421 gaagagggtt ggcgagcgag gcggagccgt tgggtctgtt gaccgaaggc gccgctggaa
      481 tcgccgtgat tgtcctcgcg gttattgctc tcgcggggac cttatccggc gctctggcgt
      541 cgatcgtgac tatcgtcgtc ggcgtcggtc tgatggttca ggcattcaat agcgcggcgg
      601 aaaactcgaa gatcatggcc gccaacgcgg ctccgattcc ggatcccggc ggtgaggtgg
      661 tggtcgacgg cctgtccggc attaccggga tcattctagg cgtcttggcg ctggttggga
      721 tcaatgctcc gtatctcgtt gcttctgcgc tgatcgtgtt tggcggtgcg ctctttctca
      781 gcggtgcgat cagcctgccg ctcagagcct ggcagaggac agccgccagc ggccagacac
      841 aagtcgtttt ctaccacggt tcggccgccg ccagcggggt ggagatcctg atcgggatcg
      901 ccgcgatcgt gttggggatc ctctcgctga tctttgttgg gtcatgggtt ctcgcgttgg
      961 tcggtttcat tgcagtcggc gctgccctcc tgatggtcag cgccacgttc agcggcacca
     1021 tgatgcggct gttcacgaca acggcctgat ttaaggccgc gacggctctt gtttcgggcg
     1081 gcttttccag cgttttcgcg ccgcgcgagc aggctgaccg cgcggcaggc tcaaggcggc
     1141 cgagacgctg gttttcgagc aacgaccttg cgctgcgcca ttgcgttgcg acacaggtgg
     1201 tcgcgcggcc agtcgaagca cggcgcggcg aacatgccgc cctgcctgat ctgtcgcgca
     1261 agaagcgaca atcatctccg aacgattatt acgcctacgc cgaagcaaac gccgattgat
     1321 tcgttacgac aagccatcaa tcgctcaggc cggaccttct tgctcccgtg cgaaccgact
     1381 cgatccctgg tggcgctctg aagtgcacgg taatctcggt aactgctcgg cagtggcttc
     1441 gggcaccaga catcgtatct cgagggtgga tacaaacacg gagagccgca gggaaaaatc
     1501 gcggacaatt tgcagcgcgt ggggttatcg tttcctgcaa gaggaatttc tgtcttacca
     1561 aagaaaccgg cgagcataat ctgatctaat cggtaccaaa tgcgtcggca tgcgagtgcg
     1621 tgagagcttg aaggtgcgga catcgcatgc catccttttt ctctcgcgca cggagccaac
     1681 cgtggatttg agatgctcgc aacttaggga ggaacccaat gaaatttcga ccgttgcagg
     1741 atcgcgttct catccgtcgc attgccgctc gatgtcaaag tcggcgaccg ggtgctgttc
     1801 ggaaagtggt cggggaccga cggcgaggat ttcgtcgtcg ctaaggagag agacatcatg
     1861 ggcgtggtcg aagagactgc gtcgcacaag gttgcgtgaa gggaggaggc tatggctgca
     1921 aaggaaatcc gctttcatac cgatgcgcgc gatcggatgt tgcgcggcat cgacattctg
     1981 tcaaacgcgg taagggtgac gttgggcccg aaaggccgca acgttgtcct cgacaaatct
     2041 ttcggagcgc cgcgcatcag caaggacggc gtcaccgtcg ccaaggagat cgagctcgcc
     2101 gacaagttcg agaacatggg cgcgcagatg gtgcgcgagg tggcctcgaa aacgagtgac
     2161 caagccggcg acggaacaac gactgcgatc gttctcgcac aaacgatcgt gcgcgagggt
     2221 gccaaggcgg cggccgcggg gatgaacccg atggacctca agcgcggcat cgat
//
LOCUS       DATLJT010000927         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3508446,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000927 DATLJT010000000
VERSION     DATLJT010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3508446"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            684..1010
                     /locus_tag="VM910_21155"
     CDS             684..1010
                     /locus_tag="VM910_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HVI65068.1"
                     /translation="MLVVTTENVAGQSVVEVKGQCFGVVVRSRGLGGNVMAGLRSIVG
                     GEIHEYTHLLEDTRRQAVDRLVQNATVMGANAVLMMRFDSSEIGQTMSEIVAYGTAAV
                     IEPLAR"
     gene            1020..1334
                     /locus_tag="VM910_21160"
     CDS             1020..1334
                     /locus_tag="VM910_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65069.1"
                     /translation="MLRAALLVIGGVMLAAAAIGALAGCRLAVIIPLAVCGAILAGGL
                     LVERWRYQGLTDDRLGRNWHATPERFVDPETGRLVTVFFNPATGERRYVADAERQLAS
                     RS"
     gene            1848..2213
                     /locus_tag="VM910_21165"
     CDS             1848..2213
                     /locus_tag="VM910_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI65070.1"
                     /translation="MFSHIMIGSNDIARSKKFYDALFAAMGAQPGTEDARGRLAYSHN
                     GSRFMVSKPIDGKPASHANGGTIGFVMKDAAQAEAWHKAGVANGGTSIEDPPGKRPNG
                     AYLAYLRDPDGNKIVAVAR"
     gene            complement(2377..>2737)
                     /locus_tag="VM910_21170"
     CDS             complement(2377..>2737)
                     /locus_tag="VM910_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI65071.1"
                     /translation="RPKRQIPIWFGGFSEAAYDRAARIGDGFLFSGRTQTEAVQIKAR
                     IVAKLRELGRDADAFGFESIQRYDRGDNQWRGDIAAWREAGGSHISVVTMGANFDTVD
                     GHIDAIKRWRDVYNSVR"
BASE COUNT          517 a          849 c          851 g          520 t
ORIGIN      
        1 gatgtcagcg agatcaagct cacgcggctc gccacgctga tcctcctgct ggcgctcggc
       61 ggatgcgccc cggtggcaac ggggcaggga caggcgccgg ccgctgctta tccgcacgat
      121 agcgggtcgg atatccgcag cggttctgac ggcagcggtg gcggcggcgg catgtagcgg
      181 gcaggcgcaa cctgggatcg ctggaccgtt gcatcgaagc cggcagcgca cggctcccct
      241 gatcgcggca aaactcctcg cccgctaatg tgtccaccta ctaataaaga tgtcactgta
      301 tgtctcgttt acaaatgtca ctagggtcgg gcgccgggcg ggcgcggagc cgcggacaca
      361 agatgttgag gcggtattct ttggccaacg aataactcgg ttgagcgctc ctgcgccgcg
      421 gggaaccctg ccacgcccga agcctagtga gccagttata tggcccggat cgcgggcagg
      481 aaaccgggga acaaataatg gccgcggctt ttgctgcagg cgccatgaag tgaccacagt
      541 cgggagcagt ggggcgacac agcaagcgcc tcgatgccga gctgctcgaa gccgtgactg
      601 tgctagccat ccccgttatt cttaggatct cggcaattgc ttagctatat cgtgcgaata
      661 attttctgca acggagtcga gggatgctgg tcgtcactac cgagaatgtc gcgggtcaaa
      721 gcgtggtcga agtcaaaggg caatgcttcg gcgtcgtggt ccgcagccgt gggcttggcg
      781 gcaatgtcat ggcagggctg cgttcgattg tcggcggcga gatccatgag tacacccacc
      841 tgctcgagga cacacgccgc caggcggtcg accgtctggt gcagaatgcc accgtgatgg
      901 gcgccaacgc cgtcctgatg atgcgcttcg actcttcgga gatagggcag acgatgagcg
      961 agatcgtcgc ctacggcacc gcggccgtga tcgagccgct cgcccgctga gggcgcaaat
     1021 tgctgcgcgc cgcgctgctc gtcatcggcg gcgtcatgct ggctgccgcc gcgatcggtg
     1081 cgctcgccgg ctgccggctc gctgtgatca ttccgttggc ggtatgcggc gcgattttgg
     1141 ccggcggcct gctggtcgag cgctggcgct accagggttt gacggatgac cgcttaggcc
     1201 gcaactggca tgccacgccg gaacgttttg tcgatccgga aaccgggcgg ctggtgacgg
     1261 tgtttttcaa tccggcgact ggagagcggc gctacgtcgc cgatgccgag cgacagctcg
     1321 cgtcccggag ctgacccggc gatgtagatt tagtcgggat ggggctgtcg ctccggcggc
     1381 cccgccttgc attcgtttcg agcgaagggc agcctctccg cgtaatgccc gaccctgccg
     1441 gccttccact aacctggcat cgctgtcggc cggcgcctcg ccgaacacac gatcattcct
     1501 gccctcgcct gacaatccgc tcacaaaatg ataacattca gctcgtaaac gagtaaaagg
     1561 ggacgtacga cagccctcgc gtccggaacc tctcgtacga agatcaggag gccattcgct
     1621 ccaattacca ccggcatagt cattcaaccg aagccgtgtg gtcctctcgt gtggcttgct
     1681 gcctaatctt ttgatgaaga tcgggatgct cgcgctccta cactcgaaga gtggagagat
     1741 tttgctcctt tgccgatcgt cggcccgtca tactgtccgg cccgcaggcc cctttccgct
     1801 tacgggcaga ggcgcgggaa tgcaatgctc ataaaggaga aaacgaaatg ttcagccaca
     1861 tcatgatcgg cagcaacgat atcgcccggt caaagaaatt ctacgatgcc ctctttgccg
     1921 caatgggggc tcaacctggt acagaggacg caagaggccg actggcctac tcccacaatg
     1981 gcagccgttt catggtatcg aagcccatcg acggcaagcc ggccagccac gccaatggcg
     2041 gcacaatcgg ttttgtcatg aaagatgcgg cgcaagccga agcgtggcac aaagccggtg
     2101 tcgcgaatgg cggcacgtca atcgaggatc cgcctggcaa gcgcccaaat ggcgcttatc
     2161 tcgcttatct tcgtgacccc gatggcaaca agatcgtcgc ggttgctcgg taagctgcct
     2221 gaccttatcg cacagcaaaa actgaggagg gccgtccttt agggaaggtc cttccgctct
     2281 ggtggacgcc ggcgaccgcc ggtgtcccca gggtgcatca gcgatttaag aaccgatgcg
     2341 ccgccctatt gggaaggagt acggcgctgc agagctctaa cggaccgagt tgtagacgtc
     2401 gcgccagcgc ttgatggcgt cgatgtggcc gtccaccgtg tcgaagttcg cccccatcgt
     2461 cacgacggat atgtggctcc ccccggcctc gcgccacgcc gcaatatcgc cccgccattg
     2521 gttgtccccg cgatcgtatc gctggatcga ctcaaagccg aaggcatcgg catcccttcc
     2581 gagctcgcgc agcttggcca cgatccgcgc cttgatctgg accgcctcgg tttgagtccg
     2641 gcccgaaaaa aggaacccgt ccccgatcct ggccgcccgg tcgtaagcgg cctcgctgaa
     2701 acctccaaac cagataggga tctgccgttt cgggcgc
//
LOCUS       DATLJT010000928         5008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2819470,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000928 DATLJT010000000
VERSION     DATLJT010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819470"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1705
                     /locus_tag="VM910_21175"
     CDS             14..1705
                     /locus_tag="VM910_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVI65072.1"
                     /translation="MYDFIIVGGGSAGSVLAHRLSAKSANKVLLCEAGQDTPPGNEPA
                     EIRDSYSGTAYFDPRFHWTELKVTTQVVSHNNPQERPPLRKYEQARVLGGGSSINGQL
                     ANRGAPTDYDEWEARGAAGWNWKGVLPYFRKVERDLDFDGPYHGKDGRISVRRIPQAH
                     WTAHSQAVAEACRLAGFAFLPDQNGEFVDGYFPVTHSNQDEQRVSAAMGYLDRETRKR
                     ANLTISTHTQVKEFLFDGTRCVGVKALVDGHEMEFRGREIVLSCGAIHSPAHLLRAGI
                     GPVGQLKDLGIPVLMGLEGVGQGLMDHPSISLSSFVRRGARMNEHTRRHIQLGLRYSS
                     GLPGVPKGDMFVAVMSKSAWHAVGARIGSLLTFVNKTYSETGQVKLASRDPKAEPIVE
                     FNLLSDRRDLDRLMDGFRKMATLQMSAPVRAVTDKPFPASYSDRVRKVGVVNAKNKVL
                     TAIAAALMDGPAALRDYVIDSFVVEGFTFDQVMRDDDALEAFVRKSAIGVWHASCSCR
                     MGRAGDPMAVVDGQGRVKGVQGLRVVDASIFPVVPCANTNFPVLMTAEKIADRMQ"
     gene            complement(1762..2634)
                     /locus_tag="VM910_21180"
     CDS             complement(1762..2634)
                     /locus_tag="VM910_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI65073.1"
                     /translation="MPAGIEIGNVTIHRIVEQEGPFFDALQFFPTLTKELFDENRKWL
                     QPRFIDAQDRLMLCIQSYIVRTPHHTIMIDSCVGNHKPRPTRSFWHMMNSDRYEKNLA
                     AAGFSVNDIDFVMCTHLHTDHVGWNTRLENGRWVPTFPKARYVFADRELDYWTRRQKD
                     DPAACPWITDSVLPIVAANRVDIVKSAHAFNDLVTLIPTPGHTIDHYSVQVGKRGADA
                     VITGDMIHSPLQARYPELGMMSDYDSAQAGESRRQLFGRFCDTSTLMCTAHFPSPSTA
                     RVVRRQDAFDLIDA"
     gene            complement(2676..3407)
                     /locus_tag="VM910_21185"
     CDS             complement(2676..3407)
                     /locus_tag="VM910_21185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65074.1"
                     /translation="MPISAADPLLDVTGLSTGYGKIRVLTGVDLTVRAGEVVALLGPN
                     GAGKTTLLRAVSGLLPWQGRVHFDGRDLAGAGPRETVKCGLAHVVEGHRVFTQLSVLD
                     NLLLAAYDLPRGERAARVEEVLALFPEIAAKRHERAAWLSGGQQQILAVAQGLVRRPR
                     LLMLDEPSAGLSPVLVDRVLVVVQRLREAGTAVLLVEQLIEKALALADRVYALARGSI
                     VLEAKTSEADLPQRLEQAYMTGGSR"
     gene            complement(3388..>5008)
                     /locus_tag="VM910_21190"
     CDS             complement(3388..>5008)
                     /locus_tag="VM910_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI65075.1"
                     /translation="LNLVVGFAGQIAIGWVALLTLGAYTTSVLAAGNVLPALPPYLAL
                     AIAALVGAIFGLIVGLPALRLRTFYFAITTLGFATIVTQVALAWQSVTGGGVGVPGPI
                     FPEPFSSQWGFYYFCFGLAAICTWMTANIAASRFGRALTAIRDAEVAAEASGIAKPAL
                     LGTVFLFSGAVAAVAGGLFASLQSYITPDAFTLDLSVLFFIAILIGGRSSILGPLLGT
                     ILLTVLPEFAAPLVAWSTFLYAVLLLVIVLAIPGGIAEMLDFKNRRPLESDRAIVPRP
                     ELVARLLDTAPDAGTLTLDQVALSFGGVRAIDGLDMEIRPGQVHGLIGPNGSGKTTTL
                     NVISGYYAPQHGAVRLNGAALPALMRHQRAHMRIARTFQTPRIVGSASVLENVMIGGT
                     IYGRATFVESLLSLPRHRCDEAMLRKTAMLALESVGLERLAAVRADRLQHAELRFIEI
                     ARGLMLRPSFLLLDEPAAGLSAEEIARLSTLLLAIARAGTGVLLVEHHPDLIFDICHH
                     ITVLNLGKTLAAGTPAEIRSHREVVNAYLGG"
BASE COUNT          917 a         1588 c         1672 g          831 t
ORIGIN      
        1 gggaaacgcc agcgtgtacg atttcatcat cgtcggtgga gggtctgcgg gatcggtcct
       61 ggcccaccgg ctctccgcca agagtgccaa caaggtcctg ctctgcgaag ccggacagga
      121 tacgccgccc ggcaatgaac ccgccgagat cagggacagc tattcgggca cggcctattt
      181 cgatccgcgc ttccactgga ccgaactcaa ggttacgacc caggtcgtca gccataacaa
      241 tccgcaggaa cggccgccct tgcgcaaata cgagcaggcg cgcgtcttgg gcggcggctc
      301 ctcgatcaac ggccagctcg ccaatcgcgg cgcgccgacc gactatgacg agtgggaggc
      361 gcgaggcgct gcggggtgga actggaaggg cgtgctgccc tatttcagga aggtcgagcg
      421 cgatctcgat ttcgatgggc cgtaccacgg aaaggacggc cgcatttcgg tccgccgtat
      481 cccgcaagcc cactggacgg cacattcgca ggccgtcgcc gaggcctgca ggctggcggg
      541 ctttgcgttc ctgcccgacc agaacggcga gttcgtcgac ggttattttc cggtcacgca
      601 ctccaatcag gacgagcagc gcgtctcggc ggcgatgggc tatctcgacc gcgagacgcg
      661 aaagcgcgcc aatctcacga tctcgaccca cacgcaggtc aaggagtttt tgttcgacgg
      721 cacgcgctgc gtgggcgtaa aggcgctggt cgacgggcac gagatggaat tccgcggccg
      781 cgaaatcgtg ctctcctgcg gcgcgatcca ttcgccggca catctgctcc gcgccggcat
      841 cggtccggtc gggcaattga aggacttggg cattcctgtc ctgatgggcc tcgagggcgt
      901 cggccagggc ctgatggatc atccgtcgat ctcgctgtcg tcattcgtcc gtcgtggagc
      961 gcgcatgaat gagcacacca ggcgccacat ccagcttggg ctgcgctact cgtcgggttt
     1021 gcccggcgtg ccgaagggcg acatgttcgt tgccgtgatg agcaagtcgg cgtggcatgc
     1081 cgtcggcgcg cggatcggat cgctgctcac cttcgtcaac aagacctact ccgagactgg
     1141 gcaggtgaag ctggcctcgc gcgacccgaa agccgagccg atcgtcgagt tcaatttgct
     1201 gtccgacagg cgcgatctcg accgcctgat ggatggcttt cgcaaaatgg cgaccttgca
     1261 gatgagcgcg ccggtgcggg cggttaccga caagccgttc ccggcgtcct actccgaccg
     1321 tgtgcgcaag gtcggagtgg tcaatgcgaa gaacaaggtg ctcaccgcaa tcgccgctgc
     1381 gctgatggac ggaccggcgg cgctgcgcga ttacgtgatc gacagttttg tggtcgaagg
     1441 cttcaccttc gatcaggtga tgcgcgacga tgacgcgctg gaagccttcg tgcgcaagtc
     1501 ggcgatcggt gtgtggcacg cctcctgttc atgccgcatg ggccgggcgg gcgatccgat
     1561 ggccgtcgtc gacggccagg gccgggtcaa gggcgtgcag ggcttgcgcg tggtcgacgc
     1621 ctcgattttc ccggtggtgc cgtgcgccaa caccaatttc ccggtgctca tgacggcgga
     1681 gaagatcgcc gacaggatgc agtgaggacg ccctctgaaa gggggaggtt ggggtagggg
     1741 tcagattcgc gacatcacac cctacgcgtc gatgaggtcg aacgcatcct gccgccgcac
     1801 cacgcgagcg gtcgacggcg agggaaagtg cgccgtgcac atgagcgtgg atgtatcgca
     1861 gaaacggccg aacaattgcc gcctcgactc ccccgcctgg gcggagtcgt aatccgacat
     1921 catgccgagc tccggatatc gggcttgcag cggcgagtgg atcatgtcgc cggtgatgac
     1981 ggcgtcggcc cctcgcttgc cgacctgcac cgagtagtgg tcgatggtat ggccgggcgt
     2041 cgggatcagc gtaacgaggt cgttgaacgc gtgcgcgctt ttgacgatat cgacgcgatt
     2101 ggcagccacg atcgggagca cggaatcggt gatccatggg catgctgccg ggtcgtcctt
     2161 ctggcgcctg gtccagtaat cgagttcgcg atcggcgaat acgtagcgcg ccttcgggaa
     2221 ggtcggcacc caccgcccgt tctcgagccg cgtgttccac ccgacatggt cggtgtgcaa
     2281 atgcgtgcac atgacgaaat cgatgtcgtt cacgctgaac ccggcggctg caaggttctt
     2341 ctcgtagcga tccgagttca tcatgtgcca gaagcttcgc gtcggtcgcg gtttgtggtt
     2401 gcccacacag ctatcgatca tgatcgtgtg gtgaggcgtc cgcacgatgt agctctggat
     2461 gcagagcatc agcctgtcct gagcgtcgat gaacctgggc tgcagccact tccggttctc
     2521 gtcgaaaagc tctttcgtca gcgtcgggaa aaactgcaac gcgtcgaaga agggaccttc
     2581 ctgctcgacg atgcggtgga tcgtgacgtt tccgatttcg attccagcgg gcaagatcgc
     2641 ctccgtcttt ttgtcaatcc agtcaggttc aggcgctagc gactgccgcc cgtcatatag
     2701 gcctgctcca gccgctgcgg caaatcggcc tcggaggttt tcgcttccag cacgatcgat
     2761 ccgcgcgcaa gcgcatagac gcgatcggcc agcgccagcg ccttctcgat caactgctcg
     2821 accaacagca ccgcggtgcc ggcctcgcgc agccgctgca ccacgaccag cacgcgatca
     2881 accagcaccg gcgacaagcc ggccgaaggt tcgtcgagca tcaagagccg cggccgccgc
     2941 accaggccct gcgccaccgc caggatctgc tgctggccgc cggaaagcca ggccgcgcgt
     3001 tcgtgacgtt tggcggcgat ttcggggaag agcgcgagca cctcttcgac acgcgcggcg
     3061 cgctcgccgc gcggcaggtc gtaggcggca agcagcaggt tgtcgagcac cgaaagctgc
     3121 gtaaacacgc gatggccctc gacgacatgg gcaagcccgc atttgacggt ctcgcgcggg
     3181 ccggccccgg cgaggtcgcg gccgtcgaaa tgcacgcgcc cctgccaggg cagcagcccc
     3241 gatacggcgc gcaacagcgt ggttttgccg gcgccgttgg ggccgagcag cgccaccacc
     3301 tcgccggcgc ggaccgtgag gtccacgccc gtgagcacgc ggatcttgcc gtagccggtc
     3361 gacagcccgg tgacatcgag cagcggatca gccgccgaga taggcattga ccacctcccg
     3421 atgcgagcgg atttcggccg gggtcccggc agccagcgtc ttgccgagat tgagcacggt
     3481 gatgtgatgg cagatgtcga agatcagatc gggatggtgt tcgaccagca gcacgccggt
     3541 gccggctcgg gcgatcgcca gcaacagcgt cgaaagccgc gcaatttcct cggccgagag
     3601 cccggctgcc ggctcatcga gcagcaggaa gctcggtcgc agcatcaggc cacgggcgat
     3661 ctcgatgaaa cgcaattcgg catgttgcag gcggtcggcc cgcaccgccg cgagccgctc
     3721 cagcccgact gattcgagcg ccagcatggc ggtcttgcgg agcatggctt cgtcgcaccg
     3781 gtggcgcggc agcgacaaca aggattcgac gaacgtggcc cggccgtaaa tcgtgccgcc
     3841 gatcatcacg ttctccagca ccgacgccga gccgacgatg cgcggcgtct ggaaggttcg
     3901 cgcaatccgc atatgcgcgc gctggtggcg catcagcgcc ggcagcgcgg cgccgttcag
     3961 cctgacggcg ccgtgttgcg gagcgtaata acccgagatc acgttcagcg tcgtggtctt
     4021 gccgctgccg ttggggccga tcagtccgtg cacctgaccg gggcggattt ccatatcgag
     4081 gccgtcgatc gcgcgtaccc cgccaaagct cagtgccacc tgatcgagcg tgagggtgcc
     4141 tgcatcgggc gccgtgtcca ggagccgcgc gacgagctcc ggtcgcggaa cgatcgcgcg
     4201 gtcgctctcg agcgggcggc ggttcttgaa atcgagcatc tcggcaatgc cgccgggtat
     4261 cgcgagcacg atgacgagca gcaacaccgc atagagaaac gtggaccagg cgaccagggg
     4321 tgctgcgaat tccggcagca ccgtcagcaa aatcgtgccg agcagcggac cgagtataga
     4381 gctgcgcccg ccgatcagga tggcgatgaa gaacaggacc gacaggtcga gggtgaatgc
     4441 gtccggcgtg atgtaggatt gcaacgaggc gaacagcccg ccggcgactg cggcgacggc
     4501 gcccgaaaac agaaacaccg ttccaagcaa cgcgggcttt gctataccgc tggcttcggc
     4561 cgcaacctcc gcatcgcgga tcgcggtcag cgcgcggccg aatcggcttg cggcgatatt
     4621 cgccgtcatc cacgtgcaga tcgcagccag cccgaagcag aaatagtaga agccccattg
     4681 cgacgagaat ggctccggga agatcggtcc aggcacgccc acgccgccgc cggtgacgct
     4741 ctgccacgcc agcgccactt gcgtcacgat cgtggcgaag ccaagcgtcg tgatggcgaa
     4801 atagaaggtg cgcagccgca gcgccggcag gccgacgatc aggccgaaga tggcgccgac
     4861 cagcgcggcg atcgccagcg ccagataggg cggaagcgcc ggcaggacat tgccggctgc
     4921 aagcacgctc gtggtatagg ctccgagcgt gagcaaggcc acccagccga tcgcgatctg
     4981 gccggcaaac ccgacgacca ggttgagg
//
LOCUS       DATLJT010000929         8228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2819567,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000929 DATLJT010000000
VERSION     DATLJT010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2819567"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VM910_21195"
     CDS             complement(<1..939)
                     /locus_tag="VM910_21195"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HVI65076.1"
                     /translation="MTDDPVVAIAGVTGAVGAEFMATMDKRGFRVGKLKALASKRSAG
                     KRLSFRGQELVIEELTENSFEGVDIALFSAGSSVSRRFSPIAVKAGAVVIDNSSAFRM
                     DPDAPLVIPEINCHRIREHKGIIAVPNCSAITALVPLWPIHRNNRIKRLIISTYQAAS
                     GAGAAAMQELLDSTRAHLAGQAYQPKVLPHPYAFNVFNHDTRIDPETGYNEEETKVIR
                     ETRKIFEDESIAIGVTCVRVPVLRAHSQAITFECERPINEQEVREILAGAPGVRIVDD
                     RAKNYFPMPIDASGQDEVLVGRIRKDLSDPSGRSIAM"
     gene            1234..2370
                     /gene="tgt"
                     /locus_tag="VM910_21200"
     CDS             1234..2370
                     /gene="tgt"
                     /locus_tag="VM910_21200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HVI65077.1"
                     /translation="MEDLPNHFELLAIDGNARTGHLTTPHGVVRTPAFMPVGTAGAMK
                     GVHWRDVRDAGTDIVLGNTYHLMLRPGAERIAALGGLQHFTGWNGPMLTDSGGFQVMS
                     LSDLRKISETAVTFRSHIDGATVELSPERSIEVQRLLGADIAMQMDECVRLPAERSDV
                     ERAMQLSLRWGERSKRAFESAPSGYMLFGIVQGGDAPELRKVSARGVVDTGFHGYAIG
                     GLAVGEPQSVMLAMIEEVVPLLPVDRPRYLMGVGTPEDLLEAIRRGIDMFDCVMPTRN
                     GRHGVAFTRFGAVNLRNARHADDPRPLDEQSGWPATRAISRAYLHHLVRSGEALGAML
                     LSEINIAYYQRLMSEIRDAISRRCFEEFYELTRAGWARRDIAPR"
     gene            complement(2380..2595)
                     /locus_tag="VM910_21205"
     CDS             complement(2380..2595)
                     /locus_tag="VM910_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65078.1"
                     /translation="MTAVAEVFSTAARRPDPRGSELPTCPVCADSMVAAEASAYLPDD
                     LITYLWTCDTCGYGFVTKHTLRRFACN"
     gene            2828..3805
                     /gene="cysK"
                     /locus_tag="VM910_21210"
     CDS             2828..3805
                     /gene="cysK"
                     /locus_tag="VM910_21210"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HVI65079.1"
                     /translation="MNAPSIAGAVHPPGRGRVFDSIVDAIGDTPIVRLRKLPQAHGAH
                     ATVLAKLEYFNPAASVKDRIGASMVVAMEKAGLINADTVLIEPTSGNTGIALAFVAAS
                     RGYRLKLVMPESMSIERRKMLAFLGAEIVLTPASQGMKGAIATAEELLRTTPNSVMPQ
                     QFKNLANPEIHRRTTAEEIWNDTGGNIDIFVAGVGTGGTITGVGQVLKPRKPSLRVVA
                     VEPEESPVLSGGQHTPHKIQGIGAGFVPDILDRSVIDEIIKINSATAIETSRALARNE
                     GIPGGISSGAAIAAALQIGRRPDSAGKTILAVVPSFSERYLSTALFEGI"
     gene            3807..4046
                     /locus_tag="VM910_21215"
     CDS             3807..4046
                     /locus_tag="VM910_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HVI65080.1"
                     /translation="MADQPRRPRTLNDARTEAEAAFKKATAKVAQAPPKTAAIPGVKE
                     QVTLRIDQDVLEFFREGGPGWQDRINEALRKAAGK"
     gene            complement(4165..5637)
                     /locus_tag="VM910_21220"
     CDS             complement(4165..5637)
                     /locus_tag="VM910_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI65081.1"
                     /translation="MRYHSILLSLICMLFTAHSALADKRVAFVVGNGAYKNVAQLPNP
                     PIDAKAMASVLRNVGFEVVEGVNLTRDKMTEKLLDFGKRAQGADVAVFYYAGHGIAIS
                     GTNYLLPIDADIKSEMDVKLGAAINIDLTLDQTMGDAKVKLVFLDACRDNPFAAKIKS
                     TATRSVSVGQGLAEMKSGEGTLIAFATGPGQTALDGQEGTNSPFTRALIAHITEPGVE
                     IQQAMTEVRAQVNEETNKGQLPWGHTNLIGSVYLNPAAPPKEAPPAAAAAPGGATTPA
                     APAVASTGSGSSSEVEVEFWRSVKDSNKPEELNAYLSSYPNGQFKSLALARIAAIENG
                     PSTTTRNLTSGVDQATFTEEANQTTEDQIGLDKGQRRDVQRRLTGLGFDIKATGKFDD
                     DTRTVIKRWQAARGYPVSGYLNRLQHKALLSEIVSATPSTTSGDDSDDSSRRVRHSGG
                     GGGGGRRVSGGGGGGGGGGANPGAFMGGVIGGMMGGMFRH"
     gene            5626..5808
                     /locus_tag="VM910_21225"
     CDS             5626..5808
                     /locus_tag="VM910_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65082.1"
                     /translation="MVAHLKSPAESKFLKAAKVPDSLHEKPGLRATKIGRYQHLSARH
                     RILRAPKLSNRLDGIA"
     gene            complement(5871..6749)
                     /locus_tag="VM910_21230"
     CDS             complement(5871..6749)
                     /locus_tag="VM910_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI65083.1"
                     /translation="MQRNQFKPKAALWMAGWLALMLVMAIAGREAVRELNVFELMEVR
                     SVLGFFLLYPMIHRNGGFKTLKTARLPLHIARNLTHCAAQLGWFFALTLIPIGQVVAL
                     EFTMPIWTAILAASFLGERMTPWKVAAIVLGIVGVVIIVRPDTGEINPGQLIALAAAV
                     GFGISMALVKLLTRTESALAIIFWMLVVQSVAGFFPTLYVWTWPSAQVWGWMVVVAIC
                     GTFSHYCLANAMRYADATIVVPMDFLRVPLTATAGWLIYGERLDVWTVLGAALILVGN
                     LLNLKGANRVPARAGT"
     gene            6920..7318
                     /locus_tag="VM910_21235"
     CDS             6920..7318
                     /locus_tag="VM910_21235"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI65084.1"
                     /translation="MRILQPAEWSKPRGFSHGVEFSGPGRWIVLAGQTGGDEKGDYSP
                     ALAAQVAVALKRIVKLLAEAGAGPEHIVRLTWYLTSRGEYEAAGSGIGAAWKETLGRN
                     FPPSTLLFIDGLVDVRAKVEIEVTAFVPAS"
     gene            complement(7473..8027)
                     /locus_tag="VM910_21240"
     CDS             complement(7473..8027)
                     /locus_tag="VM910_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65085.1"
                     /translation="MGHQRAYLAAVDGKHRDNKTDTSAYGARFRRLPLAAAILFAACL
                     SASTGGHAQSGAFAGMAGNWSGGGTVTLDDGSKERIRCRASYAVAGTSMNMTLTCASD
                     AYKFNLAANVVDQGGAVSGTWSESSRNVGGSIQGRGGGGNFEVVANSAGFNASITLRT
                     AGSKQSIGMRADSQFRAAQIALSR"
     gene            complement(7972..>8228)
                     /locus_tag="VM910_21245"
     CDS             complement(7972..>8228)
                     /locus_tag="VM910_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65086.1"
                     /translation="WNVTIVTKSGTCEPTASSTVTVTDGRISSAGANVSGSIGHEGLV
                     RVSINGAYANGQLSGNAGSGKWNGASAGIPCSGRWEASRQ"
BASE COUNT         1547 a         2534 c         2607 g         1540 t
ORIGIN      
        1 catcgcgatc gagcggccgg acgggtcgct gagatccttg cgaatgcggc cgaccaggac
       61 ctcatcctgg cccgacgcat cgatcggcat cgggaagtag ttcttcgccc gatcgtcgac
      121 gatccggacc cctggcgcac ctgcgaggat ttcgcgcacc tcctgttcat tgatcggcct
      181 ttcgcattcg aaggtgatgg cctgggaatg cgcgcgcagc accggcacgc ggacgcaggt
      241 gacgccgatt gcgatgctct cgtcctcgaa gatcttgcgg gtctcccgga tgactttggt
      301 ttcctcctca ttgtatccgg tttcaggatc gatcctggtg tcgtggttga agacgttgaa
      361 cgcgtagggg tgcggcagca ctttcggctg ataggcctgg cctgcgagat gcgcgcgcgt
      421 cgaatcaaga agttcctgca tggcggctgc tcccgcaccg cttgcggcct gataggtcga
      481 gatgatcagg cgcttgatgc ggttgttcct gtggatcggc cagagcggca cgagcgccgt
      541 gatcgccgaa cagttcggaa cagcaatgat gcccttgtgc tcacggatac ggtggcagtt
      601 gatttcgggg atcaccaggg gcgcgtccgg atccatgcgg aaggcggacg agttgtcgat
      661 gacgacggcg cctgccttga ccgcgatcgg cgaaaacctc ctggagacgc tgctgccggc
      721 cgagaacagc gcgatgtcga cgccctcgaa ggagttttcg gtcaactcct cgatcacgag
      781 ctcctggccg cgaaacgaaa gccgcttgcc tgccgagcgc ttgctggcaa gcgctttcag
      841 cttgccgacg cgaaagccgc gcttgtccat ggtagccatg aactcggcac ccacagcgcc
      901 ggtcacgccg gcaatggcaa caacgggatc gtcggtcact ttgctctcct ttgttcgagc
      961 atgatcccgg aaaaccgttc cggatcacgt tcaggcaata aaaaagcccc ggacctttgc
     1021 aggcggggct tcggttcgca tatgcggtaa agacttaggc gcgcacgact cccgaagccc
     1081 gcgggggctt gccggttttc gtcgtggttt tcgccgaagt catcatggcc gcgggatata
     1141 ggggctgcat gcatccgcgt caatgacttt tgggcccggc cggacgctca acgaggttgc
     1201 aaaaggcgca cccgttacgc gataagccct cgcatggagg atcttcccaa tcatttcgag
     1261 ctgctggcaa ttgacggcaa tgcgcgcacc ggccacctga ccacaccgca tggcgtggtg
     1321 cgtacgccgg ctttcatgcc tgtcggtacg gcgggggcca tgaagggggt gcactggcgc
     1381 gacgtacgcg acgccggtac cgacatcgtg ctcggcaaca cctatcattt gatgctgcgg
     1441 cccggcgccg agcgcattgc cgcgctgggc ggactccaac atttcaccgg ctggaacggg
     1501 ccgatgctga cggattccgg cggcttccag gtgatgtcgc tatccgatct gcgcaagatc
     1561 agcgaaaccg ccgtgacgtt ccgctcgcat atcgacggcg ccacggtgga gctgtcgccg
     1621 gaacgctcga tcgaggtgca gcggctgctc ggcgccgaca tcgccatgca gatggacgaa
     1681 tgtgtgcggt tgccggccga acgctccgac gtcgaacgcg ccatgcagtt gtcgctgcgt
     1741 tggggcgaac ggtcgaagcg cgcgttcgag tcggcgcctt ccggctacat gttgttcggg
     1801 atcgtgcagg gtggcgatgc gccggagctg cgcaaggtct ccgcccgcgg agtggtcgac
     1861 accggctttc acggctacgc gatcggcggc cttgcggtcg gagaaccgca gtcggtcatg
     1921 cttgccatga tcgaggaggt cgtgccgctg ctgcccgtgg accggccgcg ctatctgatg
     1981 ggcgttggca cgccggagga cctgcttgag gcgatcagac gcggcatcga catgttcgat
     2041 tgcgtgatgc cgacgcgaaa tggacggcat ggcgttgcgt tcacgcggtt cggcgcggtc
     2101 aatctgcgca acgcacgcca tgcggacgac ccgcgaccac tcgatgagca gagcgggtgg
     2161 cccgccacgc gcgcgatctc gcgcgcctat ctgcatcatc tcgtgaggtc gggcgaggcg
     2221 ctcggggcaa tgctgctgtc cgaaatcaac atcgcctatt accagcgcct gatgagcgag
     2281 atcagagacg ccatttcgcg aaggtgtttc gaggaattct acgaactgac gcgggccgga
     2341 tgggcgcggc gcgatatcgc gccgcgctag gcacaaaggt cagttacatg caaagcgccg
     2401 gagcgtgtgc ttcgtcacaa aaccgtaacc gcaggtgtcg caggtccaca gataggtgat
     2461 tagatcgtcc ggcaaatagg cggacgcttc cgcagccacc atggagtctg cgcaaaccgg
     2521 acaagtcggc aactcgcttc cgcgcggatc ggggcgtcgc gcggccgtcg aaaacacttc
     2581 agcaactgct gtcatcgctg gccccccttc tgcatttgcg ttccaaagct gagcataaca
     2641 tacaccaatt cgtttgacga tgtagcaaca caaatgcgtt cgttttgcgt tacctccatt
     2701 caaataaaat tttgcgatgc agcgagtttt gaactgcgag aatcttgcgc ttgcgtgctc
     2761 acgcgcaact ccactagaga tgggcgcgga ttttcgctca tatcgcgcgt ttgcaaggga
     2821 gttcctcatg aatgcgccgt caatcgctgg tgcagtgcac cctccgggcc gtggtcgggt
     2881 gtttgactcc attgtcgacg cgatcggaga tacgccgatc gttcgcctgc gcaaattgcc
     2941 gcaggcccac ggcgcacacg cgacggtttt ggcgaaactt gaatatttca atccggccgc
     3001 gagcgtgaag gaccgcatcg gcgcttcgat ggtcgtcgcg atggaaaaag cgggcctcat
     3061 caatgccgat accgtattga tcgagccgac ctcgggcaat accggcatcg cgcttgcttt
     3121 cgtcgccgcc tcgcgcggct accgactgaa actcgtgatg ccggagtcga tgtcgatcga
     3181 gcggcgcaag atgcttgcct ttctcggcgc cgagatcgtg ctgacgccgg cctcgcaggg
     3241 catgaagggc gcgatcgcca ccgccgagga attgttgcgc acgacgccca attcggtgat
     3301 gccgcaacag ttcaagaacc tcgccaatcc cgaaattcac cgccgcacca cggcagagga
     3361 gatctggaac gataccggcg gcaacattga tatctttgtt gccggcgtcg gcaccggcgg
     3421 gaccatcacc ggggtcggtc aggtgctgaa accgcgaaaa ccctcccttc gcgtcgttgc
     3481 ggtggagccg gaggagagcc cggtgctgtc gggcgggcag cacacgccgc acaagatcca
     3541 gggcatcggc gccggtttcg ttcccgacat tctcgatcgc tccgtcatcg acgagatcat
     3601 caagatcaac agcgcgaccg cgatcgagac ctcccgcgcg ctggcgcgca acgaaggcat
     3661 tcccggcggc atttcctcgg gcgctgcgat cgccgccgcg ctgcaaatcg gcaggcgacc
     3721 ggacagcgca ggcaagacga tccttgcggt cgtgccgtcg ttctcggagc gctatctttc
     3781 gaccgcgtta ttcgaaggga tctgacatgg ctgatcaacc gagaaggccc cgaacgctga
     3841 acgacgcgcg caccgaagcg gaggcggcat tcaagaaggc caccgccaaa gtcgcgcaag
     3901 cgccgccgaa gacggccgca atccccggcg tgaaggaaca ggtcacgttg cggatcgatc
     3961 aggacgtgct ggaatttttc cgggagggcg ggcccggctg gcaggacagg atcaacgaag
     4021 ccctgcgcaa ggccgcgggc aaatagctaa atccggagtc ttttgcgcgg ctctggactg
     4081 cttcgtttcg ctcgcattga cgaatttcca aagaaaaagc ccggctcttg gccgggcttt
     4141 ttcctttccg aaatgctctg ccggtcagtg acggaacatg ccgcccatca taccgccgat
     4201 cacgccgccc atgaaggcgc ctgggttggc cccgccgcca ccaccaccgc caccgccgcc
     4261 actgacgcgg cgaccacctc cgccaccgcc gccgctgtgg cggacgcggc gcgatgaatc
     4321 atcggaatcg tcgccgctgg ttgtgcttgg tgtcgccgag acgatctcgc tcaacagcgc
     4381 cttgtgctgg agcctgttga gatagccgct cacgggatag ccgcgggcgg cttgccagcg
     4441 cttgatcacc gtgcgcgtgt cgtcgtcgaa cttgcccgtc gccttgatgt cgaagccgag
     4501 gcccgtgagc cgccgctgca cgtcgcggcg ctggcccttg tcgagcccga tttgatcctc
     4561 ggtggtctgg ttggcttctt cggtgaaggt ggcctggtcg acgcccgagg tcaggttgcg
     4621 cgtcgtggtc gaagggccgt tctcgatcgc tgcaatcctc gccagcgcca gcgacttgaa
     4681 ctgaccgttg ggatagctcg acaggtacgc attaagctct tcaggcttgt tggaatcctt
     4741 gaccgaccgc cagaactcga cttcgacctc gctgctgctg ccgctgcccg ttgaggcaac
     4801 cgcgggcgcc gccggcgtcg tggcgccgcc gggggctgcc gcggccgcgg gtggtgcttc
     4861 cttcggaggc gcggccggat tgagatagac cgaaccgatc agattggtgt gaccccaagg
     4921 caattgaccc ttgttggtct cttcgttgac ctgggcacga acctcggtca tcgcctgctg
     4981 gatttcaacg ccaggctcgg tgatgtgggc gatcagcgcg cgggtgaacg gcgagttggt
     5041 gccttcctgg ccgtcaagcg cagtctggcc ggggccggtc gcaaacgcga tcagcgtgcc
     5101 ttcgccggat ttcatctcgg cgagaccctg gccgaccgac acgctccggg tcgcggtcga
     5161 cttgatcttg gcggcgaacg gattgtcgcg acaggcgtcg aggaacacca gcttgacctt
     5221 ggcgtcgccc atggtctggt cgagcgtcag gtcgatgttg atcgcggcgc ccaacttgac
     5281 gtccatttcc gatttgatgt cggcatcgat cggcagcaga taattggtgc cgctgatcgc
     5341 gatgccgtgg ccggcgtaat agaacacggc aacgtcggca ccctgggcgc gcttgccgaa
     5401 atcgagcagc ttttcggtca tcttgtcgcg ggtgaggttg acgccttcga cgacctcaaa
     5461 cccgacattg cgcagcacgc tggccatcgc cttggcatcg atcggagggt tcggtagttg
     5521 cgccacgttc ttgtaggcgc cgttgccgac cacgaaggcc acgcgcttgt cggccagcgc
     5581 cgaatgcgca gtgaacagca tgcagatcag ggataggagg atggaatggt agcgcatctg
     5641 aaatcccctg cggaatcgaa atttttgaag gcggcaaagg tgccggacag tttacacgaa
     5701 aaacccggtc tccgtgcgac aaaaatcggc cgttaccaac acctgagcgc gcgccatcgc
     5761 atcctccgtg cgccaaagct atccaatcgc ctggacggta ttgcgtgatt tgggtcacaa
     5821 aattggaggg tgacgccgag atcggccgga gagattcgag cccgggagcc ctacgtaccc
     5881 gcgcgagccg gcacgcgatt ggcgcccttc agattgagaa ggttgccgac caggatcagc
     5941 gccgcgccga gaactgtcca gacgtcgagc cgttcgccgt agatcagcca gccagcggta
     6001 gccgtcagcg gaacccgcag gaagtccatg ggcaccacga tggtcgcatc ggcatatcgc
     6061 atcgcattgg ccaggcagta gtgcgaaaaa gtaccgcaga tcgcgaccac gaccatccag
     6121 ccccagacct gcgccgacgg ccaggtccag acgtagagcg tgggaaaaaa tccggcgacc
     6181 gattgcacca ccagcatcca gaaaatgata gcaagcgcgc tctcggtgcg ggtcaacaac
     6241 ttcaccagcg ccatggaaat gccgaagccc acggcggcgg ctagcgcaat cagttgaccc
     6301 ggattgatct cgccggtgtc cgggcgaacg atgatgacga cgccgacgat cccgagcacg
     6361 attgctgcga ccttccacgg cgtcatgcgc tcgccgagaa aggatgcagc gaggatagcc
     6421 gtccagatcg gcatcgtgaa ctcgagcgcc accacctggc cgatcggaat cagtgtcagc
     6481 gcgaagaacc agccaagctg cgcggcgcaa tgagtgaggt tgcgcgcgat gtgcaacggc
     6541 aagcgcgcag tcttgagcgt tttgaagccg ccgttccggt ggatcatggg gtagagcaga
     6601 aagaatccga gcaccgaacg cacttccatc agctcgaaca cgttcaattc gcgcaccgct
     6661 tcgcggccgg cgatcgccat caccagcatc agggcaagcc agccggccat ccacagagcg
     6721 gccttgggtt tgaactgatt tcgctgcatg gatttctcgg agcgcctctg caggcagagc
     6781 agggcaggtg ggtatcggcg agcacgacaa ggtttgcaac gcgcaaatct gccgtgcagc
     6841 gatgcgcgag aaacttcgct gtgggagggc aggcgcaatc ggtgttaagg gatcgccata
     6901 actccgggag gaaaccaaca tgcgtatctt gcaaccggcc gaatggtcaa aaccgcgcgg
     6961 cttttcccac ggtgtcgaat tcagtggtcc tggccgatgg atcgtgctgg caggtcagac
     7021 cggcggcgac gagaaaggtg actattcgcc agcgctcgct gcgcaggtcg cggttgcgct
     7081 caagcggatc gtcaagctgc ttgcggaggc tggtgccggg ccagagcaca tcgttcgtct
     7141 gacctggtat ctcacgagcc gcggcgagta tgaagccgcc ggcagcggca tcggagcggc
     7201 atggaaggag acgttgggcc gaaactttcc gccctcgacg ctgctcttca tcgacgggct
     7261 cgtcgacgtg agggccaagg tcgagatcga agtgaccgcc ttcgtccctg catcgtaagg
     7321 cagtgtttgc gggcaatgat cgaccgaaag taacagcctt cgccaccggc tggcccgagc
     7381 aggagccgca gcttcctgtt gccgtcgctg accacgcaaa aagccgtgca ggcgagcgct
     7441 gcaccaattt agactttcgc taccgggccg gattagcgcg acagcgcgat ctgcgcggcc
     7501 cggaactggc tgtcagcccg catgccgatc gattgcttgc tgccggcggt cctgagcgtg
     7561 atgctggcat tgaagccggc ggaattcgcc accacctcga aattgccgcc gccgccccgt
     7621 ccctggatcg agccgccgac gttacggctg gactcactcc aggtcccaga tacggcgccg
     7681 ccttgatcga ccacgttcgc tgcaagattg aacttgtaag cgtcgcttgc gcaggtgagc
     7741 gtcatgttca tgctggtgcc cgcgaccgca taggacgcac ggcagcggat gcgttccttc
     7801 gatccgtcgt cgagggtgac ggtgccgcca ccggaccaat tgcccgccat gccggcaaag
     7861 gccccggatt gcgcgtgtcc gcctgtcgat gccgacaggc aagcggcaaa taaaatggcg
     7921 gctgccaggg gcagccgcct gaaacgcgcg ccgtaagctg atgtgtccgt cttattgtcg
     7981 cgatgcttcc catcgaccgc tgcaaggtat gcccgctgat gccccattcc actttcccga
     8041 tccggcattg ccgctgagtt gaccgtttgc ataagcacca ttgatggaga cccgaaccag
     8101 gccttcatgt ccgatgctgc cggaaacgtt cgcgccggca gaagaaatcc taccgtcggt
     8161 gacagtcacc gtcgagcttg cggtgggttc gcatgtgccg ctctttgtca caatggtgac
     8221 attccaga
//
LOCUS       DATLJT010000930         1924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2129151,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000930 DATLJT010000000
VERSION     DATLJT010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129151"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VM910_21250"
     CDS             complement(<1..347)
                     /locus_tag="VM910_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI65087.1"
                     /translation="MTSAAQLQPATPAKRILVVEDELMIRMLLEDMLGELGYTVTAEA
                     GRLDEALQAAKTADFDLAILDVNLDGEPVSPVADALVARGTPFVFATGYGERGLPEPY
                     RDRPMLKKPFQMDG"
     gene            585..1205
                     /locus_tag="VM910_21255"
     CDS             585..1205
                     /locus_tag="VM910_21255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVI65088.1"
                     /translation="MQFLLGLRRRGISDPAVLRAMDEVPREHFVENSFVDNAYADQAL
                     PIACGQTISQPYVVAFMTEQLDVRPEHRVLEVGTGSGYQAAILSRLAREVISVERYRT
                     LADTARQRLATLGYDNVDVRVGDGLAGVSEGAPYDRIMVTAAAETIPDALLGGLADGG
                     VMVLPLGPHAGPQQLVKLTKGEQGIKQEDLIGVRFVPLLPGKAREL"
     gene            1312..>1924
                     /locus_tag="VM910_21260"
     CDS             1312..>1924
                     /locus_tag="VM910_21260"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVI65089.1"
                     /translation="MSGVLEMLRSRFSPRAAVLALVTISVAACSADTSRFSDNPFASL
                     SNHEPTAGMPQAQTAQAPPPRSGTSTSVALNSGAASEATGSVLRKPTSSGNWSWDGGT
                     AITVTAGETIDTISRRHGVPASVIMQANNIAAPASLHAGQRLVIPRYKPARVATTAPP
                     RPAVPTPAPAASPAGNANVHVVAAGDTLSKIAHRYHKSVNEIAK"
BASE COUNT          334 a          603 c          640 g          347 t
ORIGIN      
        1 ccatccatct gaaacggctt cttgagcatc ggccggtcgc gataaggttc gggcaggccg
       61 cgttcgccgt agccggtggc gaagacgaac ggcgtgccgc gggcgacgag ggcatcggcc
      121 acgggcgaga cgggctcgcc gtcgagattg acatcgagga tcgcgaggtc gaagtcggcg
      181 gtcttcgccg cttgcaacgc ctcgtcgaga cgaccagcct cggcggtgac ggtgtaaccg
      241 agctcgccca gcatgtcctc gagcagcatg cggatcatca gctcgtcttc gacgacgagg
      301 atgcgcttgg ccggcgtcgc cggctggagt tgtgcggcag aggtcatctc tctcccccgg
      361 tcttgcgatc ggattcgcaa ccgaaggaca tcagatcgtc aaggcgaagt tgaggcgtcg
      421 ccgagatgca cggggcggct ttcggccccc gttgcgaggt cgccaaacga ttggatttac
      481 ggcgttttct ccgtgctatc tggccgagga cgggttttcg gctaaagtcg atcaggaggc
      541 tcggcgtgcc caagcaactg cctggcgacg acaatgtcgg ccgcatgcaa ttcctgctcg
      601 gcctgcgcag gcgcggcatc agcgatccgg cggtgctgcg cgccatggat gaggtgccgc
      661 gcgagcattt cgtcgaaaat tccttcgtgg acaacgccta tgcggaccag gcgctgccga
      721 tcgcctgcgg gcagaccatc agccagcctt acgtggtcgc cttcatgacc gagcagctcg
      781 acgtgcgacc ggagcatcgt gtgctcgaag tgggcaccgg ctcgggctat caggctgcca
      841 ttctgtcccg gctcgcgcgc gaagtgatca gtgtggagcg ttatcggacg ctggctgaca
      901 ccgcgcgcca gcggctggcc acgctgggct atgacaacgt cgatgtgcgg gtcggggatg
      961 gtcttgccgg cgtttcggaa ggggcgccct acgaccgcat catggtcact gccgccgccg
     1021 aaaccatccc cgatgcgctc ctcggcgggc ttgctgacgg cggtgtgatg gtgttgccgc
     1081 tcgggccgca cgcaggtccg cagcagctgg tcaaactcac aaagggtgaa caaggaatta
     1141 agcaggagga tttgatcggc gtacgattcg taccgctttt gcccggaaag gcgcgtgaat
     1201 tgtaacgatt ttgttcatgc ggcggcaagg ttgaggcgcc gtaatcgctt aaagtcatat
     1261 taacccgcaa ggtgttcact cggcgcttgg gttgcgtacg agtagggtgc gatgtcgggg
     1321 gttctcgaga tgttgcgctc gcggttctca ccgcgggcag cggtgctggc gctggtgaca
     1381 atcagtgttg ccgcctgtag cgcggacacg tctcgtttca gcgacaatcc gtttgcctcc
     1441 ctcagcaatc acgagccgac cgcagggatg ccgcaagcgc aaaccgcgca ggcgccgccg
     1501 ccgcgttcag gtacgagcac ctccgtcgcc ttgaattccg gcgcggcctc cgaagccacg
     1561 ggatcggtgc tgcgcaagcc tacgtcgtcc ggcaattgga gctgggacgg cgggaccgcg
     1621 atcacggtta ccgcgggcga gaccatcgat accatttcac ggcgccacgg cgtgccggca
     1681 tcagtcatca tgcaggccaa caatatcgcc gcgcccgcaa gccttcatgc cggccagcga
     1741 ttggtcattc cgcgctacaa gcccgcgcgg gtcgcaacca cggcgccgcc gcgcccggcc
     1801 gtgccgacac ccgcgccggc cgcgagcccc gccggcaatg cgaatgttca cgtggtcgcc
     1861 gccggcgaca cgctgagcaa aattgcgcat cgctaccaca agtcggtcaa cgagattgcc
     1921 aagg
//
LOCUS       DATLJT010000931         4992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2358782,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000931 DATLJT010000000
VERSION     DATLJT010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358782"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VM910_21265"
     CDS             <1..168
                     /locus_tag="VM910_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-butanediol diacrylate esterase"
                     /protein_id="HVI65090.1"
                     /translation="GSLTWAGLYNTYYWIDPASSVAGVIMMQILPFADARALTLYRQF
                     ERSIYRVLKTA"
     gene            complement(113..964)
                     /locus_tag="VM910_21270"
     CDS             complement(113..964)
                     /locus_tag="VM910_21270"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HVI65091.1"
                     /translation="MKLKTIGGLLLALICAIGMTVSQSRADNRTAFLSDHPLCEPGTQ
                     WDGAACTAVPSCPVGQRWSGTGCVAVECGSGYRLEGSFCVPDTCPTGQRLSGNRCVAI
                     QCNTGFRLEGSTCMPLNCPAGQRLNGNNCEQIVCGPGEELIDNTCTASGRSRYLALAV
                     GQTNHLGYGAAWDKQSVQEAQDEAMRLCQNQIPAGSCKIILSGPEQCLALWWTPTGTG
                     GVPAARKPRMPPLQAAPTSTSGRNACWRDPGAIREAENLARQAGRGGLTPSSARGRCC
                     ARIADRG"
     gene            complement(1202..1975)
                     /locus_tag="VM910_21275"
     CDS             complement(1202..1975)
                     /locus_tag="VM910_21275"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HVI65092.1"
                     /translation="MSNFNLESVTSVHHWTDTLFSFTTTRNPTFRFRNGEFTMIGLKV
                     GERPLLRAYSVASANYEDKLEFFSIKVPDGPLTSRLQHLREGDEIIVSRKATGTLVID
                     NLEAGRNLYLVGTGTGLAPFLSVIKDPETYERFDKIVLLHGCRRVKELAYGEMITEKL
                     PNDELIGELVSGKLIYYPTVTRDPFRNRGRITDLITSGKLFADIGLAPLDPAHDRVMI
                     CGSPALVHDTRVLLTERGFVEGNHGEPAQFVVEKAFAER"
     gene            2125..3321
                     /locus_tag="VM910_21280"
     CDS             2125..3321
                     /locus_tag="VM910_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65093.1"
                     /translation="MAHDAPQSTGPRKLVIRNIGLLLSGALEKPILDADAIVAENGKI
                     TGIGRLKDLNTEGATTIVDANGTTVAPGLIDSHVHPVTGDWTPRQSQINWIDSYLHGG
                     VTTMISAGEVHMPGRPRDVIGLKAMAIFAQRAFWTLRPGGVKVHAGAPVIECEMVEDD
                     FKELAAAGVKLLGEVGLGGVKDGPTARKMVSWARKYGIQSTIHTGGPSIPGSGLIDKD
                     VVLEADTDVVGHINGGHTALPDDQVRCICEGCKRGLELVHNGNERSALYTLRTAREMG
                     DLSRIILGTDAPAGSGVQPLGILRMVAMLSSLGEVPAETAFCFATGNTARMRALDCGL
                     VEIGRAADFVIMDKAQHSPGKTLLDSVRLGDLPGVGMTIIDGIVRTQRSRNTPPATKV
                     PDIVGH"
     gene            complement(3382..4176)
                     /locus_tag="VM910_21285"
     CDS             complement(3382..4176)
                     /locus_tag="VM910_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVI65094.1"
                     /translation="MTEQDTEEAPSRPLWIAAGVVALALHVGGAAFALTHLKADEAEE
                     DLGAPAIEIGLELTSPRKEVTDLPPGPDSDASVASPALAEQKAELKQTDLPKDTSTET
                     EEADRIVTQNEVKKPVEEEAKVATVQTQASQESVAAEATASPSSETIPEGVRSVAPVH
                     GIGESAQRVRATWQKALIAHLDKHKRYPADRIAKSAEILVSFVLDRMGHVLSASIVKG
                     SGDPAFDDAALAMVRRSDPVPQPPPLVADEGLNFTLPVLFKVKGKS"
     gene            complement(4194..4640)
                     /gene="exbD"
                     /locus_tag="VM910_21290"
     CDS             complement(4194..4640)
                     /gene="exbD"
                     /locus_tag="VM910_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HVI65095.1"
                     /translation="MSISLADSDLDDDGDFGETHEINVTPFIDVMLVLLIIFMVAAPL
                     STVDLPIDLPTSTATPQKKPEKPTYVSIKPDLALALGETPVKRVDLVRALDAMPDASK
                     DRFIFLRADRAVPYGEMMDVLEILRAGGYAKIKLVALEGVAEAAKP"
     gene            complement(4645..>4992)
                     /locus_tag="VM910_21295"
     CDS             complement(4645..>4992)
                     /locus_tag="VM910_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVI65096.1"
                     /translation="RRIRFGMGLLATIGATAPFVGLFGTVWGIMNSFIGISKSQTTNL
                     AVVTPGIAEALLATAIGLAAAIPAVIIYNHFARVTKGHLELVARASGAASRLLSRDLD
                     RGHGGVPLARAAE"
BASE COUNT          893 a         1631 c         1540 g          928 t
ORIGIN      
        1 ggcagtctga cgtgggccgg cctctacaac acctattact ggatcgatcc ggcctccagc
       61 gtcgccggcg tcatcatgat gcagatcctg ccctttgccg acgcgcgcgc gctcaccctc
      121 tatcggcaat tcgagcgcag catctaccgc gtgctgaaga cggcgtgagc ccccctctcc
      181 ccgcttgccg ggcgagattt tcggcctcac gtattgcacc aggatcccgc caacacgcat
      241 ttctgccgct tgttgacgtt ggcgcagctt gcagcggcgg catccttggc ttcctcgcgg
      301 ctgggacgcc gcctgttccc gtcggagtcc accacagtgc aaggcactgc tcgggaccgg
      361 acaggatgat cttgcagctt ccggctggta tctggttctg gcacagcctc atggcctcat
      421 cctgagcttc ctgcacgctc tgcttgtccc aggccgcgcc gtagccaaga tggttggttt
      481 gtcccaccgc cagcgcgaga taccggctcc ggcccgaggc cgtgcaggtg ttgtcgatca
      541 gttcctcgcc cggtccgcaa acgatctgct cgcagttatt gccgttcagc cgttgcccgg
      601 cggggcaatt gagcggcata caggtcgagc cctccagtcg aaagcccgtg ttgcactgga
      661 tcgcgacgca tctgttgccg ctgagccgct ggccggttgg acaggtgtcc ggcacgcaaa
      721 acgaaccctc cagacgatag ccgctgccgc actcgaccgc cacgcagccg gtgccgctcc
      781 agcgttggcc gaccggacag cttggcaccg ccgtgcaggc ggcaccgtcc cattgcgtgc
      841 ccggctcgca caggggatga tcggacagaa acgctgtgcg gttgtccgcc ctggattggc
      901 tcacggtcat accgatggcg cagatgagcg ccagcagcag cccgccaatc gttttcagct
      961 tcacggcaat tccaccctgc cctgttctac cgtgaggggt tttggacgat catcattcgt
     1021 ccgaaccggc gcgctatgga ttcgcagtcg ctgaaatctt acggtttgcc cgccatcagc
     1081 cgcaagaccg atcttgatcg gcctgcctgc agttggggat tacgcggcgc tgctcgggga
     1141 ggctccgcaa tctactaccg ttcgagacac gcaggccctg tgcgagccgg cgactaaatc
     1201 tctatcgttc cgcgaaggcc ttctcgacca cgaactgcgc gggctcgcca tgattgccct
     1261 cgacgaagcc gcgctccgtg agcagcacgc gcgtgtcatg gaccagcgca gggctgccgc
     1321 agatcatcac gcggtcatgc gcaggatcga gcggagcaag tccgatatcg gcaaacagtt
     1381 tgcctgacgt gatcaggtcg gtgatacggc cgcggttgcg gaaagggtcg cgcgtcaccg
     1441 tcggataata gatgagcttg ccgctcacga gctcgccgat cagctcatcg ttgggcaatt
     1501 tttcggtgat catctcgcca taggcgagct ctttcacgcg acggcagccg tgcagcagca
     1561 cgatcttgtc gaaacgctcg taggtctcgg gatccttgat gacggacagg aacggcgcaa
     1621 gccccgtgcc ggtgccgacc agatagaggt tgcggcccgc ctcgagattg tcgatcacga
     1681 gcgtgccggt cgccttgcgg ctgacgatga tctcgtcgcc ctccctgagg tgctggagtc
     1741 gcgaagttag cggaccgtcc ggcaccttga tcgaaaagaa ctcgagcttg tcctcatagt
     1801 tcgcgctggc gacgctgtag gcccgaagca gcggccgctc gccgaccttg agcccgatca
     1861 tggtgaactc gccgttgcga aagcggaagg tgggattgcg ggtggtggta aagctgaaca
     1921 gcgtgtcggt ccagtggtga acgctggtga cgctttccag attgaaattg ctcatcgctc
     1981 gattcccctc accggcaaaa cccggttgac cggcaaactc gttcgtacac gatcgttcgt
     2041 atacaaatga ataatccagc ttgatcaacc cgtcaagtcg ggcgcaaaat cgtcaacgat
     2101 ttcagtggag aaggaccccg acccatggcc catgacgcgc cgcaatcgac cggcccccgc
     2161 aagctggtca tccgcaatat cgggctcctg ttgtccggcg ccctggagaa gccgatcctc
     2221 gatgcggacg cgatcgttgc ggaaaatggc aagatcaccg gcatcggccg cctcaaggac
     2281 ctcaacaccg agggcgcaac caccattgtc gatgccaacg gcacgacggt cgcgccgggc
     2341 ctgatcgaca gccacgttca ccccgtcacc ggcgactgga cgccgcggca gagccagatc
     2401 aactggatcg acagttacct ccacggcggc gtcaccacca tgatctccgc cggcgaagtt
     2461 catatgcccg gccgcccgcg cgacgtgatc ggcctgaagg cgatggcgat cttcgcgcag
     2521 cgcgcgttct ggaccttgcg ccctggcggc gtgaaggtgc atgccggcgc gcccgtcatc
     2581 gaatgcgaga tggtcgagga cgatttcaag gagctcgccg cagccggcgt gaagctgctc
     2641 ggcgaagttg gcctcggtgg cgtcaaggat ggcccgaccg cgcgcaagat ggtttcctgg
     2701 gcgcgcaaat acggcattca aagcacgatc cacaccggag gaccgtcgat ccccggctcc
     2761 ggcctgatcg acaaggacgt ggtgctggaa gccgacaccg atgtggtcgg ccatatcaat
     2821 ggcggccaca ccgcgctgcc cgacgaccag gtccgctgca tctgcgaggg ctgcaagcgc
     2881 gggctcgagc tcgtgcacaa cggcaacgag cgctcggcgc tctacaccct gcgcaccgcg
     2941 cgcgagatgg gtgatctttc ccgcatcatc ctgggcaccg atgcgccggc aggctccgga
     3001 gttcaaccgc tcgggatttt gcgcatggtt gcgatgctgt cctcgctcgg cgaagtgccg
     3061 gccgaaaccg ccttctgctt tgcgaccggc aataccgcgc ggatgcgcgc gcttgattgc
     3121 ggccttgtcg agatcggtcg cgccgccgac ttcgtcatca tggacaaagc gcagcactcg
     3181 cccggcaaga cgctgctcga cagcgtgcgg ctcggcgacc tgcccggcgt gggcatgacc
     3241 atcatcgacg gcatcgtgcg aacccagcgt agccgcaaca caccgccggc tacgaaagtg
     3301 cctgacatcg tcgggcatta gagcacgaga gccttatgtc gaatcgacat cgcgctctgg
     3361 tttagcgttc gagcaaggct gtcagctctt gcccttcacc ttgaaaagca ccggcagcgt
     3421 gaaattcaac ccctcgtcgg cgaccagtgg cggaggctgt ggcacgggat cggagcggcg
     3481 gaccatggcg agcgccgcat catcaaacgc aggatcgccg gatcccttga cgatgctcgc
     3541 cgacagcaca tgccccatgc ggtccagcac aaagctgacc aggatttcgg cgctcttcgc
     3601 gatccggtcc gccggatagc gcttatgctt gtcgaggtgc gctatcagcg ccttctgcca
     3661 tgtcgctcgc acccgctggg cgctttcgcc gatcccatgc acaggcgcaa ccgaacggac
     3721 gccctccggg attgtctcgg agctcggcga agcggtcgcc tcggcagcaa cagattcctg
     3781 ggaagcctgg gtctgcacgg tggcgacctt ggcttcttcc tccaccggct tcttcacctc
     3841 gttttgcgtg acgatccggt cggcttcctc ggtttcggtc gacgtgtcct tcggaaggtc
     3901 ggtttgcttg agctcggcct tctgctcggc gagcgcgggc gacgcgaccg aggcatccga
     3961 atccggcccg gggggcagat cagtcacctc cttccggggc gacgtcagtt cgagaccgat
     4021 ttcgatcgcg ggcgcgccga gatcctcttc agcctcgtcg gccttcagat gcgtgagcgc
     4081 gaacgcggcg cctccgacgt gaagcgccag cgcgacgaca ccggcggcaa tccacagggg
     4141 ccgcgaaggt gcctcttcgg tgtcttgttc ggtcatcgcg atcggccgtg acttcagggt
     4201 tttgcagcct ccgctacgcc ctcgagcgca acaagcttga ttttggcgta accgccggcg
     4261 cgcaggattt ccaatacatc catcatctcg ccatagggca ccgcgcgatc ggcgcgaagg
     4321 aagatgaaac gatccttgct ggcatcgggc atggcatcga gcgcgcgcac gagatcgacg
     4381 cgtttgaccg gggtttctcc aagcgcgagc gccagatcag gcttgatgct gacataggtc
     4441 ggcttttccg gcttcttctg cggtgtcgcc gtcgacgttg gcagatcaat cggcagatcg
     4501 acggtcgaaa gcggcgcggc gaccataaag atgatcagca gcaccagcat gacgtcgatg
     4561 aagggtgtga cgttgatctc gtgcgtctcg ccgaaatcgc cgtcgtcatc caggtcgctg
     4621 tccgcaagcg agatactcat cacgttactc cgccgcccgc gccagcggca cgccgccgtg
     4681 accgcgatcg agatcgcggg acaacagccg cgacgccgcg cccgacgcgc gcgcgacgag
     4741 ttccagatga cctttggtta ctcgggcgaa gtgattgtag atgatcacgg ccggaatcgc
     4801 ggcagcgagg ccgatcgctg tcgcaagcag cgcctctgcg atgcccgggg tgacgacggc
     4861 gagattggtc gtctgcgatt tcgaaattcc gataaaactg ttcatgatgc cccacaccgt
     4921 tccgaacagc ccgacgaacg gcgcggtcgc gccgatcgtg gccaacagcc ccatgccgaa
     4981 ccggatgcgg cg
//
LOCUS       DATLJT010000932         4129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3048920,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000932 DATLJT010000000
VERSION     DATLJT010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048920"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..479
                     /locus_tag="VM910_21300"
     CDS             45..479
                     /locus_tag="VM910_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65097.1"
                     /translation="MEREPSAADIIARLELRPHPEGGYYRETFRDTRVDAQGRASSTS
                     IYFLLTRGDRSHWHRIDATEVWHYYAGSALTLQIAHDGGTQHTVTLGPDLAAGERPQA
                     IVPSSAWQCAETTGDWTLLGCTVAPGFEFANFELAPKDWKPG"
     gene            complement(532..1401)
                     /locus_tag="VM910_21305"
     CDS             complement(532..1401)
                     /locus_tag="VM910_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVI65098.1"
                     /translation="MSPRTATLIGLTAILMWSLLSVLTVATGTIPAFQLAAMTFAIGA
                     LVGSLTWIGRTEAVKALRQPPVAWLVGVGGLFGYHALYFAALRLAPPAEAGLLNYLWP
                     LLIVLFSALLPGERLAPHHVIGALLGLVGTVVLFAGNASGFTRGQVPGLIAAFAAAFV
                     WASYSVLSRRLKSVPTDAVTGFCAATALLAALVHGLVETTVWPETLLQWIAIAALGIG
                     PVGAAFYAWDIGMKRGDIRVLGAASYATPLLSTAFLMLAGFAKASAAIAIAAVLIAGG
                     GLLAAKDMFRKRL"
     gene            complement(1550..2275)
                     /gene="phbB"
                     /locus_tag="VM910_21310"
     CDS             complement(1550..2275)
                     /gene="phbB"
                     /locus_tag="VM910_21310"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HVI65099.1"
                     /translation="MARIALVTGGTRGIGAAISKTLKAAGYKVAANYGGNDAAAEKFK
                     AETGISVYKWDVSSFDACAAGVKKVEADLGPIDVLVNNAGITRDGAFHKMTPEQWSAV
                     INTNLGSLFNMCRQVIEGMRARKFGRIVNISSVNGQKGQFGQTNYSAAKAGEIGFTKA
                     LALENAKGGITVNAICPGYINTEMVQAVPKEVLEKSILPLIPVGRLGEPEEIARAVMF
                     LAADEAGAITGSTLSVNGGQYMA"
     gene            complement(2468..3646)
                     /locus_tag="VM910_21315"
     CDS             complement(2468..3646)
                     /locus_tag="VM910_21315"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVI65100.1"
                     /translation="MSDDVVIVSAARTPVGSFNGAFATMPAHDLGAVAIKAALIRAGV
                     EPGRVSEVIMGQILTAAEGQNPARQASIAAGIPVESPAWGINQLCGSGLRAVALGYQA
                     LLNGDSDIVVAGGQESMSMAPHAQHLRGGVKMGSVDLVDTMIKDGLWDAFNGYHMGNT
                     AENVARQYQITRAQQDEFAVASQHKAEAAQKSGKFKDEIVPVTIKTRKGDIIVDADEY
                     PRHGATLDSMSKLKPAFEKDGTVTAGSASGINDGAAAVVLMTAKQAAKEGRKPLARIV
                     SWGQAGVDPKIMGTGPIPASRTALKRAGWSVGDLDLIEANEAFAAQACAVNKDLGWDT
                     SKVNVNGGAIALGHPIGASGARVLVTLLHEMQKRDSKKGLATLCIGGGMGIAMCLARD
                     "
     gene            4063..>4129
                     /locus_tag="VM910_21320"
     CDS             4063..>4129
                     /locus_tag="VM910_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HVI65101.1"
                     /translation="MAKSDQPTTIKKYANRRLYNTG"
BASE COUNT          764 a         1334 c         1261 g          770 t
ORIGIN      
        1 gccgacgtgt tcggcgagct gcgcgaacgc aagaacaagt cctgatggaa cgcgagccga
       61 gtgctgccga catcatcgcg cgtctcgagc tgagaccgca cccggaaggc ggctattatc
      121 gcgagacgtt tcgcgacacg cgcgtcgacg cgcagggccg cgcaagctcc acctcgattt
      181 atttcctgct cacgcgcggc gatcgctcgc actggcaccg aatcgatgcg accgaggtct
      241 ggcactatta tgcaggcagc gcactgacct tgcagatcgc tcacgacggc ggcacgcagc
      301 atacggtgac gcttgggccc gaccttgctg cgggcgagcg gccgcaggcg atcgttccat
      361 cgtcggcatg gcaatgcgcg gagaccaccg gcgactggac gctgctcgga tgcaccgttg
      421 cgccgggatt cgaatttgcc aatttcgagc tggcgccgaa ggactggaag ccaggttgat
      481 gacgacgtca ttgcggagga gccgcgaggg cggcgaaccg gcaatgactg ttcagaggcg
      541 cttgcgaaac atatccttcg ccgcgagcag gccgccgccc gcgatcagga ccgcggcgat
      601 ggcgattgca gcgcttgctt ttgcaaaacc cgccagcatc agaaacgccg tcgacagtag
      661 cggcgtcgca taggaggccg ctcccagcac gcggatgtcg ccacgcttca tgccgatgtc
      721 ccaggcataa aacgcggctc ccaccggtcc gatgccgagt gctgcgatcg cgatccattg
      781 caacagggtc tccggccaca ccgtggtttc gacgagcccg tggacgagcg ccgccagaag
      841 cgccgtcgcc gcgcaaaagc ccgtgaccgc gtccgttggc accgatttga gccggcgcga
      901 cagcacggaa tagcttgccc atacgaacgc agcggcaaag gctgcgatca atcccggcac
      961 ctgtcctcgc gtaaaaccgc tcgcgtttcc ggcaaacaac acgaccgtac cgacaagccc
     1021 gagcagcgca ccaatgacgt gatgcggcgc cagccgctcg cccggcaaca gcgcggagaa
     1081 cagcacgatc aggagcggcc agagataatt gagcaggccc gcttccgccg gcggcgcaag
     1141 gcgcagcgcc gcgaaataga gcgcgtgata gccgaacagg ccgccgaccc cgaccagcca
     1201 ggcgacaggc ggctgccgca gcgccttgac cgcctcggtg cgcccgatcc aggtcagcga
     1261 acccaccaac gcgccgatcg caaacgtcat cgctgcgagc tggaatgcgg ggatcgttcc
     1321 ggttgcgacc gtcaggaccg atagcagcga ccacatcagg atcgcggtca gcccgatcag
     1381 ggtggcggtg cggggggaca tagccgaaat ttttctccgt catggccggg cttgtccgac
     1441 catccacgtc ttggcagcaa tatcggtccc aagacgtgga tgcccggcac atggccgggc
     1501 atcacgggtc atttgcacga tcgcgcgtag cgacgcaacg cctgcggcgt cacgccatgt
     1561 actggccgcc gttgaccgac agcgtcgagc cggtgatggc gccggcctcg tctgccgcga
     1621 ggaacatcac ggcgcgggcg atctcctccg gctcaccgag ccggccgacc gggatcagcg
     1681 gcaggatgga tttttccagc acctccttcg gcaccgcctg caccatttcc gtgttgatat
     1741 aacccgggca gatcgcattc acggtgatgc cgcccttggc gttctcaagt gccaacgcct
     1801 tggtaaatcc gatttcgccg gccttcgccg ccgaataatt ggtctggccg aactgcccct
     1861 tctgcccgtt gaccgaggag atgttgacga tgcggccgaa cttacgggct cgcatgcctt
     1921 cgatgacctg acggcacata ttgaacagtg aaccgagatt ggtgttgatg acagcgctcc
     1981 attgctcggg cgtcatcttg tggaacgcgc cgtcacgcgt gatgccggca ttgttgacga
     2041 gcacgtcgat cgggccgaga tccgcttcga ccttcttgac gcccgcggcg caggcgtcga
     2101 acgacgagac atcccatttg tagacggaga tcccggtctc ggccttgaac ttctcggcgg
     2161 cagcgtcatt gccgccatag ttggcggcga ccttgtagcc ggcagccttc agcgtcttgc
     2221 tgatggcggc accaatacct cgcgtacctc cagtgaccaa cgcaatacgt gccatgttgg
     2281 aatccttccc ttgacgtttc ctcttggcgc cttcaacgcg acgctctttc tgtaacgtcg
     2341 aattatgcga gtggtttgac caacatcaag aacaaaacgc ccggcgaggc cgccgggcgt
     2401 tctttttctt tggttcagcg ccgagcaatc gcgaagggat atccttttcg caacggctgc
     2461 cgacgtttta gtcgcgcgca aggcacatcg cgatacccat gccgccgccg atgcacagcg
     2521 tggcaaggcc tttcttcgaa tcgcgcttct gcatctcatg cagaagcgtg accagcacgc
     2581 gtgctccaga cgcgccgatc gggtggccga gtgcaatcgc gccgccgttg acattgacct
     2641 ttgaggtatc ccagcccagg tccttgttga ccgcgcaggc ctgcgcggcg aaggcttcgt
     2701 tggcctcgat cagatcgagg tcgccaacgc tccagccggc cctcttcaac gcggtgcgcg
     2761 aggccggaat cggccccgtg cccatgattt tcggatcgac gccggcctgg ccccacgaca
     2821 cgatgcgcgc gagcggcttc ctgccttcct tggccgcctg cttggcggtc atcagcacga
     2881 ccgcagcggc gccgtcattg atgccggaag cgctgccggc agtgacagtg ccgtctttct
     2941 caaaggcagg cttcagcttg ctcatggagt cgagcgtcgc accgtggcgc ggatattcgt
     3001 ccgcgtcgac gatgatgtcg cccttgcggg tcttgatggt gaccggcacg atctcgtcct
     3061 tgaacttgcc ggacttctgc gcagcttcgg ccttgtgctg ggaggcgacc gcaaattcgt
     3121 cctgttgggc gcgggtaatc tgatattgcc gcgcgacgtt ctctgcggtg ttgcccatgt
     3181 ggtagccgtt gaaggcatcc cacagaccgt ccttgatcat ggtgtcgacg aggtccactg
     3241 agcccatctt gacgccgccg cgcagatgct gggcgtgcgg ggccatgctc atggattcct
     3301 ggccgccggc cacgacgatg tcggaatcgc cgttgagcag cgcctgatag ccgagcgcga
     3361 ccgcgcgcag gccgctgccg cagagttggt tgatgcccca ggccgggctt tccaccggaa
     3421 tgccggcggc gatcgaagcc tgccgcgccg gattctggcc ctccgcggcg gtcaggattt
     3481 ggcccatgat gacttcggac acgcgtccgg gctcaacgcc cgcgcggatc agcgcggcct
     3541 tgatcgctac ggcgccaaga tcatgggccg gcatggtggc aaaggcaccg ttgaaacttc
     3601 cgaccggggt gcgggcggcg ctgacgatga cgacatcgtc tgacatgggc atctcctggg
     3661 gcttaaggtt cttgaagaaa tcggcgggcg ccggaatggc aagccagtct ctcaccccat
     3721 cctgttaacg tgcttgcggc atgtcaatcg gctggtcacg aatttgcagg ctcgcgcatt
     3781 caattcggcg cgtcttgata cctcgatacc ttgcgcgccg ccagagattg cttcattcaa
     3841 cgcaagatct ctccggtggt ggccgagccc cgaggctttc gaacagcgct cgagcgctct
     3901 tcacccgcaa atccggaaaa atgctgcgca ataataaaat gccgccaaat taaccgtggt
     3961 gcacaaaacg gtagccgaac ccctttgaaa atgcttattt tgttgcgttc tgccctgcgg
     4021 cgaacgccgc tcggttccct tcctcggttt gtgtgtgagc ccatggcgaa atctgaccaa
     4081 cccacaacta tcaagaaata cgcaaaccgg cggctttata ataccggta
//
LOCUS       DATLJT010000933         5448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1900609,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000933 DATLJT010000000
VERSION     DATLJT010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900609"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..983
                     /locus_tag="VM910_21325"
     CDS             297..983
                     /locus_tag="VM910_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVI65102.1"
                     /translation="MTAAPTLVFDLDGTLVDTAPDLIDALNFVLEREGLPAVPLQAAR
                     TMIGAGARKLIERGLELEGRAASTVDINRMTADFIEYYAAHIADGSRPFDGLEIALDE
                     LSARGFRFAVCTNKLEWLSKRLLDELGLSTRFAAICGADTFGVAKPDPAILQQTVSRA
                     AGKMSSTIMVGDAGTDIGVARRAGVPVIGVSFGYTDVPIVELKPDRLIHHMRDLPSAV
                     ESLAALQNIL"
     gene            1111..1339
                     /locus_tag="VM910_21330"
                     /pseudo
     CDS             1111..1339
                     /locus_tag="VM910_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1449..2483
                     /gene="moaA"
                     /locus_tag="VM910_21335"
     CDS             1449..2483
                     /gene="moaA"
                     /locus_tag="VM910_21335"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVI65103.1"
                     /translation="MNGPVSTPLPALSRPMIDPFGRTISYLRVSVTDRCDLRCFYCMA
                     EDMTFLPKADLLTLEELDRLCSAFIARGVTKLRLTGGEPLVRRNVMSLVRSLSRHLTS
                     GALRELTMTTNGTQLARFASELADCGIRRINVSLDTLDAAKFRAITRWGELDQVLAGI
                     EAARAAGLAVKINAVALKNLNEDEIPSLIEWAHGSGMALTLIEVMPMGEIGAGRIDQY
                     VPLSLVRARLSKQFTLTDLHESTGGPARYVRVGETGGTLGFITPMTHNFCESCNRVRI
                     TCTGTIHTCLGQEDASDLRKPLRASPDDELLSQAIDRAIGTKPKGHDFIIDRRHNRPS
                     VSRHMSVTGG"
     gene            2776..3315
                     /locus_tag="VM910_21340"
     CDS             2776..3315
                     /locus_tag="VM910_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HVI65104.1"
                     /translation="MRPLLAVSTAIDQLNERIGNFCNLLVLISCLVSAGNAMIRYAFG
                     YSSNGWLEAQWYMFAILVMFGASYTFKRNEHVRVEIFYLFLSERGQLWLDLIGTLFCL
                     IPACLLLAYLSWPFFMQAYSVGEMSGNAGGLVRWPIKFVIPAGFVMLALQGVSEVIKR
                     VAALQGYVTIDARYERPTQ"
     gene            3312..4913
                     /locus_tag="VM910_21345"
     CDS             3312..4913
                     /locus_tag="VM910_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVI65105.1"
                     /translation="MITLEMMPPLMFGGLVLAMLIGYPVAFTLAAVGLSFGFLSIYLG
                     FFDLNFLQAIPGRVFGSVLSNELLLAIPFFTFMGAVLERCGLAEDMLDSMGQLFGPIR
                     GGLGYSVIIVGFILGAITGTVAAQVIAMALISMPVMIRYGYNMRYITGVLAASGTITQ
                     LVPPSLVLIVLADQLGKSVGDMYLGAWGPSVFQIALFAGYTFLLGMFKPSHVPPVPKE
                     ARTLTGWPLWKKCLMGIIPSAVLIFVVLGTMMLGLATPTEAGAMGAVGAIVLAAIHHK
                     DFSRAGSKILIVGVIAAGIGTMIGIFYSENLIFKIAFAITYFAVVWICLEAVRIPDLR
                     DLIKQGYQTTMRITCMVTFILIGSTCFSVVFLGVSGGVWLEHMLTSLPGGVWGFLAFI
                     NLFIFFLAFFLDFFEIAFIILPMIAPAAQKILAPVVGPDAALIWFGVMLCVNMQTSFL
                     HPPFGFALFYLRGVAPKEVKSSDIYWGAMPWIGLQALMVALVIAFPITVTALLDKPVS
                     VDLNKVKIEVPQIELPPLDLGPPKQ"
     gene            complement(4996..>5448)
                     /locus_tag="VM910_21350"
     CDS             complement(4996..>5448)
                     /locus_tag="VM910_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65106.1"
                     /translation="AEWVGPYDDEKLGFYKVAPHYYYPGWWEGGPMLLAMINLDKWNA
                     LPKYYQSVLEQAGHLANNWMMARYDQANPMALRKLLANGTRLHAFSPPIMEASFRAAK
                     ELHNEVSATNANFKKVYESLTNFSNNGYAWFQVAEVGYDNFMARHFQS"
BASE COUNT          938 a         1785 c         1628 g         1097 t
ORIGIN      
        1 gcgcacatcg tcgagcgcgc gcgccgccgc ctgccgtttc aacgcttcca tgtccaccgg
       61 caaaaccctg cccactagaa accggcgctt tctagcgtcg ttttgcggcc gggaatagcc
      121 gccaaacggc ccgaatgccg cgactttcaa tcaatttgcg gtccccctgc cggcaaaggc
      181 cacccctcgc ttaagggcgg acattcatta ttccctgtta ggtcttgacc gattttcaca
      241 aatccctcag ccccccacct tgcgtgcggg cgctttggtc gctagcgctt gctcagatga
      301 ccgctgcgcc caccctcgtc ttcgacctcg acggcacgct ggttgatacc gcgcccgacc
      361 tgatcgacgc gctcaatttc gtgctggaac gggagggatt gccggccgtt ccactgcagg
      421 ctgcgcgtac catgatcggc gccggcgcgc gcaagctgat cgagcgcggg ctcgagctcg
      481 aaggccgcgc cgcaagcacg gtcgacatca accggatgac tgccgacttc atcgagtact
      541 atgccgctca catcgcggac ggctcgcgcc ccttcgacgg gctggaaatt gcgctcgacg
      601 aattgtccgc gcgcggcttt cgctttgcgg tctgtaccaa caagctggaa tggctgtcga
      661 agcggttgct cgatgagctt ggactgagca cgcgatttgc ggcgatctgc ggtgccgata
      721 cgttcggcgt ggccaagccc gatcccgcca tcctgcaaca gacggtatcg cgtgccgccg
      781 gaaagatgtc ctcgaccatc atggtgggcg acgccggaac cgatatcggg gttgcgcgcc
      841 gtgccggcgt tcccgtcatc ggcgtcagct tcggctatac cgacgtgccg atcgtggagc
      901 tgaaacccga ccgactgatc catcacatgc gcgatctgcc gtctgcagta gagagcctgg
      961 cagccttgca aaatatatta taacctattg atcaggcgat agattcttgg ccgaagcgag
     1021 gtcggcaagg ggatgtgact ggcgcagtgg ttcagtccag acgcgagtgc agaaactgaa
     1081 tttctgggaa accggtcacc tacgcgctgc aagttccagg cggccaccgt ttcagtgaac
     1141 gtggtccgca accccggcga ttacgccaac ccggcatcga cgaccaccga cccccaaccc
     1201 cgtcttcgcg gagctgcaac cggcaggccc gccgccgaag gttcacacca cgccgaaaac
     1261 gaagaagccg aagaccacgg cgccggacac cgccgactcg ccattcccga atccgaacgc
     1321 cccaccgccg acctgctgac gtctcgcagc gccgcattca cctttgcgga ttgtggggtg
     1381 atcccatcgt gcttagattg cgggaagatt tccgagcagc ggcatctgcg tccccaaggc
     1441 ttgttggcat gaacggaccc gtatcgaccc ccctcccggc gctgtcccgt ccgatgatcg
     1501 atccgttcgg gcggaccatc agctatttgc gggtgtcggt caccgatcgc tgcgatctcc
     1561 gctgttttta ttgcatggcg gaagacatga cctttctgcc gaaggccgac ctcctgacgc
     1621 tggaagaact cgaccgtctg tgctcggctt tcatcgcaag gggggtcacg aagctgcggc
     1681 tgaccggcgg ggaaccactg gtccggcgca acgtgatgtc gctggtgcgc tcgctgtcac
     1741 ggcatcttac gagcggcgcc ttgcgcgaac tgacgatgac caccaacggc acgcagctcg
     1801 cgcgcttcgc ctccgagttg gcggattgcg gcatccgccg catcaacgtg tcgctggata
     1861 cgctggacgc cgcaaaattc cgcgccatta cccgttgggg cgaactcgac caggtgctgg
     1921 ccggcatcga ggccgcacgc gcggccggcc ttgcggtcaa gatcaacgcg gtggcgttga
     1981 aaaatctcaa tgaggacgag atcccgtcgc tgatcgaatg ggcgcacggc agcggcatgg
     2041 cgctgacgct gatcgaggtg atgccgatgg gcgagatcgg cgccggccgc atcgatcagt
     2101 atgtaccgct gtcgctggta cgcgctcgcc tctccaagca attcaccctg accgacctcc
     2161 acgagagcac cgggggaccc gcgcgctacg tccgcgtcgg cgagaccggc ggcacgctcg
     2221 gcttcatcac gccgatgacc cataatttct gcgagtcctg caaccgggtg cggatcacct
     2281 gcaccgggac gatccatacc tgcctcggcc aagaggacgc ttccgatttg cgcaagccgt
     2341 tgcgggcctc gcccgacgac gagctgctca gccaggccat cgaccgcgcc atcgggacca
     2401 agccgaaggg ccacgacttc attatcgatc gccgccataa ccggccgagc gtcagccggc
     2461 atatgagcgt gacaggcggc tagaacggcg ccccccgaat attcccggat gtgactgatt
     2521 taccaattga cgcggcgata gcgcgctgaa atggtgcggc tcacttctac gcctgctcgg
     2581 ccggaaaaag gccgctgcgc cgcagggcgc agccaagacg gcaagaaggg acacgtggga
     2641 gggaccattc ttgcaagtgc tcctgcatac gagccgtgcc ttccctcgcc tgcgcgccga
     2701 ccgcgcgggc aggcaagcca tgcctgtcga gacaatcggc gcacgccacc ggaccgcgcc
     2761 gccgcggaga acacgatgcg tccattgttg gcggtgagca cggccatcga tcagctcaac
     2821 gagaggatcg gcaacttctg taacctgctg gtgctcatct cctgcctggt cagcgccggc
     2881 aacgccatga tccgctacgc gttcggctac tcttccaacg gctggctcga ggcgcagtgg
     2941 tacatgttcg cgatcctcgt gatgttcggc gcctcctata ccttcaaacg caacgaacac
     3001 gtgcgggtcg agatcttcta tctgttcctg tcggagcgcg gccagctctg gctcgatttg
     3061 atcggcacct tgttctgcct gatcccggcc tgcctgttgc tcgcctatct gtcctggccc
     3121 ttcttcatgc aggcctattc ggtcggcgag atgtccggca atgccggcgg gctggtccgc
     3181 tggccgatca agttcgtgat tcccgccggc ttcgtcatgc tggcgctgca aggcgtctcg
     3241 gaagtcatca agcgcgtcgc tgccctccag ggttacgtca cgatcgatgc gagatacgag
     3301 aggccgactc aatgattacg ctggagatga tgccgcctct gatgttcggc ggcctggtcc
     3361 tggcgatgct gatcggctat ccggtcgctt tcacgctggc ggccgtcggg ctctcgttcg
     3421 gattcctgtc gatctatctc ggcttcttcg acctcaactt cctgcaggcg atccccggcc
     3481 gcgtgttcgg cagcgtgcta tccaacgaac tgctgctcgc gatcccgttc ttcaccttca
     3541 tgggcgcggt gctcgaacgc tgcgggcttg ccgaggacat gctggattcg atgggccagt
     3601 tgttcggccc gatccgcggc ggtctcggct attcggtcat catcgtcggt ttcatcctcg
     3661 gcgccatcac cggcacggtg gcggcgcagg tgatcgccat ggcgctgatc tccatgccgg
     3721 tcatgatccg ctatggctac aacatgcgct acatcaccgg cgtgctggct gcctccggca
     3781 ccatcacgca gctcgtgccg ccgtcgctgg tcctgatcgt gcttgccgac caactcggca
     3841 agtcggtcgg cgacatgtat ctcggcgcat ggggaccgtc ggtgttccag atcgcgctgt
     3901 ttgccggcta caccttcctg ctcggcatgt tcaagccgag ccacgttccg ccggtgccca
     3961 aggaggcgcg gacgctgacc ggctggccgc tgtggaagaa atgcctgatg ggcatcatcc
     4021 cctcggcggt gctgatcttc gtcgtgcttg gcaccatgat gctgggtctt gcgacgccga
     4081 cggaagccgg agccatgggc gcggtcggcg cgatcgtgct cgcagccatc catcacaagg
     4141 atttcagccg cgccggcagc aagattctga tcgtcggcgt catcgccgcg ggcatcggca
     4201 cgatgatcgg aatcttctac agcgagaacc tcatcttcaa gattgccttc gccattacct
     4261 atttcgcggt ggtctggatc tgcctcgagg cggtccgcat ccccgacctg cgcgacctga
     4321 tcaagcaggg ctatcagacc accatgcgca ttacctgcat ggtgacgttc attctgatcg
     4381 gctcgacctg cttttcggtg gtgttcctcg gcgtctccgg cggcgtctgg ctcgagcaca
     4441 tgctgacctc gctgccgggc ggcgtctggg gcttccttgc cttcatcaat ctcttcatct
     4501 tcttcctggc gttcttcctc gacttcttcg agatcgcctt catcatcctg ccgatgatcg
     4561 cgccggccgc gcagaagatt ctcgcgccgg tcgtcgggcc cgacgccgcg ctgatctggt
     4621 tcggcgtcat gctctgcgtc aacatgcaga cctcattcct gcacccgcca ttcggatttg
     4681 cactcttcta tctgcgtggc gtcgcgccca aggaggtcaa gagctccgat atctattggg
     4741 gtgcgatgcc ctggatcggg ctgcaggcgc tgatggtcgc gctggtgatc gccttcccga
     4801 tcacggtgac cgcactgctc gacaagccgg tcagcgtcga tctcaacaag gtcaagatcg
     4861 aggtgccgca gatcgaactg ccgccgctcg atctcggtcc gccgaagcaa taaccgaaac
     4921 tcttcgtggg caagcaaaaa ccccggggca aatgccccgg ggttctctct ttcacgaaag
     4981 cgcggatcgc agcgatcagc tctggaagtg gcgggccatg aaattgtcgt aaccgacttc
     5041 ggcgacctgg aaccaggcat agccgttgtt ggagaagttg gtcagcgact catagacctt
     5101 cttaaagttg gcgttcgtcg ccgagacctc gttgtgcagc tccttcgcag ccctgaacga
     5161 ggcctccatg atcggtggcg agaaggcgtg cagcctggtg ccgttggcga gcagcttgcg
     5221 caacgccatc ggattggcct gatcatagcg cgccatcatc cagttgttgg cgagatggcc
     5281 ggcctgctcg agcacgctct gataatactt cgggagcgcg ttccacttgt ccaggttgat
     5341 catggcaagc agcatcgggc cgccctccca ccagccggga tagtagtagt gcggcgcgac
     5401 cttgtagaaa ccgagcttct cgtcgtcata ggggccgacc cactcggc
//
LOCUS       DATLJT010000934         3294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_522301,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000934 DATLJT010000000
VERSION     DATLJT010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522301"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..415
                     /locus_tag="VM910_21355"
     CDS             89..415
                     /locus_tag="VM910_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65107.1"
                     /translation="MVENPLAFESSKTRRICDAFDDAWAFLQGLGCDLTDASQSLATR
                     TILAKRIIEMADQGLTDVAALRDNALAFLQHNPPFRLIAKGSAKNHRSLGSLARRARG
                     KAVETV"
     gene            499..621
                     /locus_tag="VM910_21360"
                     /pseudo
     CDS             499..621
                     /locus_tag="VM910_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            complement(1465..1767)
                     /locus_tag="VM910_21365"
                     /pseudo
     CDS             complement(1465..1767)
                     /locus_tag="VM910_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
     gene            1803..>3294
                     /locus_tag="VM910_21370"
     CDS             1803..>3294
                     /locus_tag="VM910_21370"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI65108.1"
                     /translation="MNRKTEHSSRARGKLKIGDDWNAITIIALSQTSPLKAIAELVET
                     SIDAKARTVTITRGREHRRHFLSIRDDGEGVPRDNAGRPDFRYVATHVCDSVKRRLKA
                     EGTSGLQGEFGIGLLSFWTVGDELSITSTGDDRRAYQMVMRKGDPTYVVTPMRALFGD
                     RGTEVRIAPLLDGIRGLSGEKIQWYLAAELRDRIRQTGVRINVVDRLARKQYAVEPRQ
                     YEGRLLHQLPALHTRIGDMYAELYLGDPSDSNRVALFRRGTRVVEDLALLDDLAHPPW
                     TLRCLQGHVDAPFVNLTPGTRSGLIYDTAYATLCEALQPLERKLVEVIEEQRRAEEEQ
                     ASQEQLRTIQRAFREALLALPAEEYDWFDIHARTFRSKLDGATVASSPSLSHEVNGVV
                     LGAAEPELGEDRQRQFFEFAGPLFSVTVSPASTMVRVGEAKELRALPRDRSRRRVEHD
                     VTFQWQIVEGPGALAGMHNQAVTFLAPDEPGLTRVKVTVRQRDVVCA"
BASE COUNT          585 a         1058 c         1064 g          587 t
ORIGIN      
        1 cgggccctta atccactgca tcgcagcacc cctttgagcg tccctccgtt ctcgcgccga
       61 agcatgtcga gcttgaggag tttgcgagat ggttgaaaat ccgctggcgt ttgaatctag
      121 caagacacgc aggatttgcg acgcgttcga tgatgcctgg gcgttcttgc agggtcttgg
      181 ctgcgacctc acggacgcat cccagtccct cgcgacgcga acgatcctgg ccaagcgcat
      241 catagagatg gccgatcagg gcctgacaga cgttgcagca ctccgtgata acgccttggc
      301 cttccttcaa cacaatccgc ccttccggct gatcgcaaag ggctccgcta agaaccaccg
      361 gagcctcggc agtctcgctc gtcgagctag gggcaaagca gttgagacag tgtgagcggt
      421 ttcgccgtct gcttgcacgc ggcggactga taccctacgc ccgcgccaac aggttgctca
      481 ctgacttggc ataccactgg ccgcaacgtc gccgaagttg aaatcgacct cgatatctgc
      541 gtcgtgcagc tcgtcaacta cacggccgtc gacgacgtcg ccactgtcat caatcccatg
      601 attgtcgagg gccagctgca tcttccacga ttgctcaggg ttagttgttc gatctgccgc
      661 gggacgttgc gccgtcggcc ccggcccaat ccgcccctga tccatgcgag tatcgttcca
      721 gagcttccgg gcagccttgg ccggatcgat gccggcatcc ttccaccagg cggcttcatt
      781 gcctacgcgg atgatggatg cggcagagcg gcaccgcaaa ctcgtcgctc gccttgcgac
      841 ccaaggcctg cggtcgcatg aagggtcgga cggcttggga ccacagatga ggcagggctg
      901 ttgggcgacg tagcgaaggt gcgctttgtt gcgatagcgg cggggagcgg gaaccgcgag
      961 cgtacttttg tcgacgcggg cgggctgatc attaccggca tcacccgaag cagcgtccct
     1021 gcccatcgtc tcctcgccgg ctaacgaacg tgaacacaga gagccgctgc cgatggaggg
     1081 ctcggagctt tcggacggca agagcgcgga cagtcgcagt tcgaaggcaa cctccaacag
     1141 cctggcgtcg gcggcggtga gctggttctt ggcagcgccc gctgcgctca atccgtggcg
     1201 ctgtcctctg atggcagggt tgcgatctcg cccaagagcg gatcacgcag gcaagccgac
     1261 tgatcggggg aggggaccgg tgggctcgag ggctttgccc ctccgcgggc gcggccgttg
     1321 caatggctgc cgggcggcag ctgtaatcca cccgcttgtc catcgatgtt gcgatctacg
     1381 gctcgccggt ttcctcccgt ctgccccgcc cgaccccgcc atccgcgttt ttccaagcgg
     1441 tcccgtggcc ggtgcagggg ctcaaatgtc gggcgcatca aggtcttcct cgcccgcaat
     1501 ccccaggagt gcgaataggg catagcgccg ggcataggtc agcgccgccc ccatgcggtg
     1561 aggattggca gtctcggcca aggggcaaac cggccagtcc gaggcgatcc actcgcccga
     1621 agcatgggcc agcatggtgg tcagattgac catcccgact gccggatcga tggcgctcgt
     1681 ctgcgcattt gagctgctct tacgggagtg gctccgaaat tcccagcccc cgcgcggctt
     1741 cagtcgcttc tcgccggatc tctccatgat aataagaagc gggcaaaaca ccgagcggta
     1801 gcatgaaccg aaagacagaa cactcgagcc gggcgcgcgg caagctgaag atcggcgacg
     1861 actggaacgc catcactatc atcgccctgt ctcagaccag cccactgaag gcgatcgccg
     1921 agctggtcga aaccagcatt gatgcgaagg cgcgcactgt gacgatcacg cgcggccgcg
     1981 agcacaggcg ccattttctc tcgatccgcg acgatggcga aggcgtgccg cgcgacaacg
     2041 ctggccggcc ggatttccgc tacgtggcga cccacgtctg tgattccgtc aagcgccggc
     2101 tcaaggccga agggacctcg ggcctacagg gcgagttcgg gatcggcttg ttgagcttct
     2161 ggacggtggg cgacgagctc agcataacgt cgaccggcga cgaccggcgc gcctaccaga
     2221 tggttatgcg taaaggcgat ccgacctatg tggtcacgcc catgcgcgcg ctgttcggcg
     2281 acaggggcac ggaagtgcgg atcgctccgc tgctcgatgg catccgcggt ctgtcgggag
     2341 aaaagatcca gtggtatctc gcggcggagc tgcgcgaccg tatccggcag accggcgtcc
     2401 gtatcaacgt cgtcgaccgg ctcgcgcgta agcagtacgc cgttgagccg cgccaatacg
     2461 agggccggct gctgcatcag ctgccggcgc tgcacacaag gatcggcgac atgtatgccg
     2521 agctgtatct cggcgatcca tccgacagca atcgcgtggc attgttccgg cgcggcacgc
     2581 gcgtggtcga ggaccttgca ctgctggacg atcttgccca tccaccgtgg accttgcgct
     2641 gcctgcaggg ccacgtcgat gcgccgttcg tcaacctcac gccaggcacg cgttcgggcc
     2701 tcatctacga cacggcctat gcgacgctgt gcgaggcgct gcagccgctt gagcgcaagc
     2761 tcgtcgaggt catcgaagag cagcgccgcg ccgaggaaga gcaggcgagc caggagcagc
     2821 tgcgcacaat ccagcgtgcg ttccgggaag cgctgctagc gttgccggcc gaggaatatg
     2881 actggttcga cattcacgcc cggacgttcc ggtcgaaact cgacggcgcg accgtggcga
     2941 gttcgccttc gctctcgcac gaggtcaacg gagtcgttct cggggcggca gagccggaac
     3001 tcggcgagga ccggcaacgg cagttcttcg agttcgccgg accgcttttc agcgtgacgg
     3061 tttctccggc atcgaccatg gtgcgggtcg gcgaggccaa agagctgcgg gccctgccac
     3121 gcgatcgttc gcggcgccgc gtcgagcacg atgtcacgtt ccagtggcag atcgtagaag
     3181 ggccgggcgc cctggccggg atgcacaacc aagccgtcac cttcctggcg ccggacgagc
     3241 ccggcctcac gcgtgtgaag gtcacggtgc gccagcgcga cgttgtttgt gccg
//
LOCUS       DATLJT010000935         2191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_749455,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000935 DATLJT010000000
VERSION     DATLJT010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749455"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VM910_21375"
     CDS             complement(<1..255)
                     /locus_tag="VM910_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVI65109.1"
                     /translation="MLVEQFRQDIGNVSSTVDERQLRQKSHDFHWYSPVLTPQLQNCL
                     ADVVVQPRSEEEIAAVVVAAVKHRIPLTVRGGGTGNYGQSV"
     gene            complement(582..1829)
                     /locus_tag="VM910_21380"
     CDS             complement(582..1829)
                     /locus_tag="VM910_21380"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI65110.1"
                     /translation="MLAATAILLAGSYITYRLVDPLPPRHFVIAAGIADSGYDNFAKR
                     YARILARNGIDLEIRNTAGAVENLDLLRDPASKVQAALTTLGFAHPGDADHLASLGGV
                     FDAPIFVFYKSAQPVTRLAQLRGKRLAIGTAGTSVRPLILKVLEAAGAADAPTRFVDL
                     DYDRAVDALIAGEIDVAIFPSQLDTGLLRRALAAPDVRLMNVVQAAAIAKTVPGIKHL
                     VLWQGLVDLTRDIPDADVNLLASGNSLLVRKDLHPALQYLLLEAIREVHSPPGPFNRL
                     GEFPAEQSDDLPLSATAQSFYRSGPTFWQRYTSFWLTSLVDRILFFVVPVVVALIPVI
                     GFASRLYRWLAVRRIVRLHRALGGLERELAQREDNAQLAQHQARLSEIESAVGTLKVA
                     PPFELDLHRLRIHLRMVQDHLKS"
BASE COUNT          449 a          657 c          699 g          386 t
ORIGIN      
        1 tacagattgc ccgtagttgc ccgtaccacc gccccgaacg gtcaatggaa ttcgatgctt
       61 gacggctgcc actaccacag cggcgatttc ctcctcgctc cgcggctgaa ccacaacgtc
      121 cgccaagcaa ttctgaagtt gtggggtaag cactgggctg taccagtgaa agtcgtggga
      181 cttttggcgt agttgacgct catcaaccgt cgaactgaca ttgccgatgt cctggcgaaa
      241 ctgctcgaca agcattcgac ttgccatttt ggcctcttca aacttcggcg gcggggctca
      301 aactgcctgc tgcgccacga acggcgtcac gcgcattctt ttaaagtaac caattagatc
      361 ggccaaacaa ccgttttcaa agcctgcgca gtatttccga acgtacgcta ctgacgcaaa
      421 gctccgagac gaagcgctct catgatgcag atggcgtgca tccacgccgc agcttgagtc
      481 cggttttggc acaaggcgga catgatgccg atcgatttcg cgacccccgc cgtacgcggg
      541 acggttggta gacgccgcgt gctggagcga cggggctagg tttagctttt cagatggtcc
      601 tggaccatgc gcaggtggat ccggagccga tgcaggtcga gctcgaacgg cggagcaact
      661 ttcaacgtgc ccacggcgga ttcgatctcg gagagccgag cctgatgctg ggcaagctga
      721 gcgttgtctt ccctctgcgc gagctcgcgc tcgagccctc ccagcgccct gtgcaatcgg
      781 acgatacgac gaacggccag ccagcgataa aggcgcgacg caaagccgat gacggggatg
      841 agcgccacga cgacggggac gacgaagaac aggatgcggt cgaccagcga ggtcagccag
      901 aacgacgtgt atcgttgcca gaacgtgggg cccgagcggt agaagctttg cgcggtcgca
      961 gagagcggca gatcgtcgga ttgctcggcg ggaaactcac cgagccgatt gaacggcccc
     1021 ggcggcgagt gaacttcgcg gatcgcttcc agcagcagat attgcaaagc cgggtgcagg
     1081 tcctttcgca ccagcaggct attgccagaa gcgagcaggt tgacgtccgc gtccggaatg
     1141 tcgcgcgtga gatcgacgag accttgccac aacaccaggt gcttgatgcc tgggaccgtc
     1201 ttggcgatgg ccgcggcctg cacgacgttc atcaaccgca cgtcgggcgc cgcgagcgcg
     1261 cggcgcaaga ggccggtgtc gagctggctc ggaaagatcg ccacgtcgat ttcgcccgcg
     1321 atcaacgcgt cgacggctct gtcataatcc agatcgacga aacgcgtggg cgcatcggca
     1381 gcgccagcgg cctcgaggac cttaaggatc agcgggcgga cgctggttcc cgccgtgccg
     1441 atggcaaggc gcttgccgcg cagctgagcc aatcgcgtga ccggctgcgc gctcttgtag
     1501 aaaacgaata tcggcgcatc gaacacgccg cccagcgaag cgagatggtc cgcatcgccg
     1561 gggtgagcaa agccgagcgt ggtgagcgcc gcctgcacct tcgaggccgg gtcgcgcagc
     1621 aaatccaggt tttccaccgc gccggcggtg ttgcggatct ccaagtcgat gccgttgcga
     1681 gccagtatgc gggcatagcg cttggcgaag ttgtcatacc cggaatccgc tatgccggcg
     1741 gcgatgacga agtgacgagg cggcagcgga tcgaccaatc gataggtgat gtacgagcct
     1801 gcgagcagga tggcggttgc ggcgagcacg atgatcggaa aactccgtgg cctaggccat
     1861 gtcggtgcga ctgcgcgtcg ccacggaggg actctcatgg tcttaggcac gcgaccgcgc
     1921 cgccggcgag caaggtcatg aactcgcgcc gtttcaagga tcgaattgca ttctgtcccc
     1981 aacgagcaca gggaccgatt gcaggatatc gaattcgcaa tggtcaagcc aatgtccgct
     2041 tcacccccga aagcggacat tgagcgacac gcgcctggca tgtccgcttt gggtcaaagg
     2101 cggcgaggac catcagcgcg gggctggctt cttggctttg aggatgtgat cagcgtagcc
     2161 ggccgcccac cgccacaaat cttgccccaa a
//
LOCUS       DATLJT010000936         1851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_522541,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000936 DATLJT010000000
VERSION     DATLJT010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522541"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1188)
                     /locus_tag="VM910_21385"
     CDS             complement(163..1188)
                     /locus_tag="VM910_21385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI65111.1"
                     /translation="MRPPLRVGTVYDFRNTLESGMDMPSLYAAIMDQVVMLDGLGLDL
                     VWFTEHHFLDDGYLPSWVPMAGAIAARTKRVRLSSDVCLLPFNHPLRLAEDLAVLDNI
                     SNGRIEVGVGMGYAPHEFRGFGLPASRRVSLTDEDIEVLRQCFIGEKFSFHGKRYDFD
                     DVVIRPRYVQPGGPPLWIAAMSEPGAQRTARNDSNFLPQGAREKVLDPWRAMLKASGR
                     NPDNYRVGIIRSCLVTDDAEHDWPAVRAAERYRGQIYRSFNKDKSAAGGVSGNTREAS
                     IPQTWVVGNVEHCVTELTSFVREYGITDLVTWAVPPGMRPEQMNGSLERFVRDVAPRV
                     KAAAAVA"
BASE COUNT          328 a          598 c          590 g          335 t
ORIGIN      
        1 tcaaggaacg gctggcggct gacgatccgg tcggtctgcc agatcgaatc cacgccgccg
       61 gcctcgcaca tgtcgatcca gcgccagtac gccgctgcgc cggaaaacgg aaactccgca
      121 cagcccaatc cgatcccgac acccatgcgt cgcctttccg ccctaggcca ccgccgccgc
      181 ggctttgacg cgcggcgcga cgtcgcgcac gaagcgttca agactgccgt tcatctgctc
      241 tggccgcatg ccgggcggca ctgcccaagt gacgagatcg gtgatgccgt actcgcgcac
      301 gaaggatgtc agctcagtca cgcagtgctc gacgttgccg accacccaag tctgcgggat
      361 gctggcctcg cgcgtgttgc ccgaaacgcc gcccgcggcg ctcttgtcct tgttaaagct
      421 gcgatagatc tggccgcgat agcgctcggc ggcgcgcacg gccggccagt cgtgctcggc
      481 atcgtcggtt acgaggcagg agcggatgat gccgacacga tagttgtcgg gattgcggcc
      541 cgacgccttc agcatcgcgc gccacggatc gagcactttt tcccgcgcac cctgcggcag
      601 gaagttgctg tcattgcgag ccgtacgctg tgcgccgggc tccgacatcg ccgcgatcca
      661 gagcggcgga ccgcccggct gcacgtagcg cggcctgatc acgacgtcgt cgaaatcgta
      721 gcgcttgcca tggaaactga acttctcgcc gatgaagcac tggcgcagca cctcgatgtc
      781 ttcgtccgtc agcgacacac ggcgcgaggc gggcagaccg aagccgcgaa actcgtgcgg
      841 cgcatagccc atgccgacgc cgacctcgat gcggccgttg ctgatattgt ccagcaccgc
      901 gagatcctcg gccagccgca ggggatggtt gaacggcagc aggcagacgt cgctcgacag
      961 ccgcacacgt ttggtgcgcg ctgcgatcgc gcccgccatc ggaacccacg aaggcaggta
     1021 gccgtcgtcg aggaagtggt gctcggtgaa ccacacgaga tcgagtccga gcccgtccag
     1081 catgaccacc tggtccatga tcgcggcata gaggctcggc atgtccatgc ccgattccag
     1141 cgtgttgcgg aaatcgtaga cggtgccgac gcgaaggggt ggacgcatga cgctcgttct
     1201 ccgctcgtca ggatgtttgt gtggcttcag cgcggcggcg ctctgcgcct tggtggcgaa
     1261 caacaggact aagtaaaggc gacgtgaggt gccaaccgat cagacggccg cccgcgcggc
     1321 gagcatttcg gcaatcgctt cggaacggtt ttccggcttc gacgtcattg cgcggatcgc
     1381 cgccgcagta taacgccctt gcaaaatgta gatgtcatat tggtctcctg tccaacgtgc
     1441 tcccggttgc cgctcatcgt gacagacgag ggcagtaata gcagttcgga tccctccacg
     1501 gtagcgagcg atttcggcgg agttggtcgg gggtgcataa ccgatcgccc gctggttctg
     1561 cgtagaagct cagccgccgt tgtcatttgg acgggctccg caacttcgct aagtcatgcg
     1621 gacaaagccc ccgatgctcc tgaggttcgc ttctgtcgaa gcggactccc cgagtcacgc
     1681 tttcggggcc gggccacaca tgcaacggcg agagttcatt gcggggacgg cggccactgc
     1741 agtcctgagc tttggttccg tcgggtgatg gactcacaga ctctttagag cgccgtcacg
     1801 agggccgcgc tttcatccag catgggcaaa atcgcgtctc ggggcttggg c
//
LOCUS       DATLJT010000937         8708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2359378,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000937 DATLJT010000000
VERSION     DATLJT010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359378"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VM910_21390"
     CDS             complement(<1..243)
                     /locus_tag="VM910_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI65112.1"
                     /translation="MSVSKLAAGVTALAIGAAVVTALAANPEVKTDPAAAAAVAANSE
                     ANADPAAPAAAKSDRLSVRPPLKPAAPPPVTSACPQE"
     gene            complement(736..1395)
                     /locus_tag="VM910_21395"
     CDS             complement(736..1395)
                     /locus_tag="VM910_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65113.1"
                     /translation="MLKASETGLIAESELALARRDLSEDQYAQEFECSFDAAIVGSYY
                     GKLMARAEQDQRIAPVPYDPAALVWTSWDLGIRDATAIWFAQVIGREIRIIDYYEASG
                     VDLGHYVREIGARPYVYAGHIVPHDAQAKELGTGKSRLEVLQSLGLKNISIAPLHRVE
                     DGINAVRVFLPKRWFDAKKCARGIDALKLYRAEYDDKLQALRPSRCTTGPRTRPTRSA
                     T"
     gene            1409..1894
                     /locus_tag="VM910_21400"
     CDS             1409..1894
                     /locus_tag="VM910_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65114.1"
                     /translation="MLFRLRAPPQLEEGVAALGRADEDRPALPIGQRRPDDLRPHARV
                     HVGVFVEHDTVEIDAAQRIGIVGAIEPHLPSVGIIDAQLGFVRARPDRPHRRHGVAQI
                     VPRHRLRLLEEGREIGEARAYTQALGCRALQVVHATVLPARRCVTMQAERSVLLWEVM
                     N"
     gene            complement(2163..2741)
                     /locus_tag="VM910_21405"
     CDS             complement(2163..2741)
                     /locus_tag="VM910_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65115.1"
                     /translation="MGLKIFAVLALCLAMTAPGLAATIVDTQLIRAAFFSDACRQQAE
                     ALGISRTSDANALLAAFAGLRRQPANSPAAEGKRDDCSVSAVLLINLLRDNGIDAELV
                     SATMAPADPIDRVLVYVPVLDRYFDPALPLEEQSLVDLLVRERAERKHILGPALAGSA
                     RDACPSTCLHVYTAAANAMPRVRVKTETIRGR"
     gene            3510..4982
                     /locus_tag="VM910_21410"
     CDS             3510..4982
                     /locus_tag="VM910_21410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVI65116.1"
                     /translation="MRNGREPPPAWIVARLNSYRWCVVGTVCVGAFMGQVDSSIAQML
                     LPRLELEFGARLSTVSWVAVAYLLAMAAFLPIFGRLADMVGRKLLYTGGFLLFVLSSA
                     LCGLAPSLPVLIAFRVAQGIGAALLSSNSVAIVVTAAGPAQRGRALGVLSAAQAVGLS
                     AGPALGGLVLDLLDWRWVFWINVPIGLAGTVLGWFVLPPTQDLPADSRFDWKGAFLIA
                     PALTAFVAVLHEGYAWGATSPALLGCALLAVILLTLFVRAEQRADAPLIDLTLFRQSA
                     FSAGNVAGLMGYAALFGLFFLMPFIFVRAYRDSTFAAGLRLSIVPVMLGMVAPIAGAL
                     YDRLGPRLLTVSGMLICVVALLLLFAVLDGAPASLPLVMLALAVFGIGQGLFISPNNS
                     AVVAAAPASLTGEAGGLLNVMRSCGISIGVAAASSLLAWRLAVRTGSGHNTLHAETRH
                     LLAAGRDVIVLIGSYAAMAGAISLARLPSRRPGNAKDASA"
     gene            complement(5057..5284)
                     /locus_tag="VM910_21415"
     CDS             complement(5057..5284)
                     /locus_tag="VM910_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65117.1"
                     /translation="MSITPYLGDFDASPETKRVLGVALEMTRVSLGLGDNFADGIIAR
                     RIVQLAKAGERNPDLLCKGALDELCGHLYGD"
     gene            5546..5707
                     /locus_tag="VM910_21420"
     CDS             5546..5707
                     /locus_tag="VM910_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65118.1"
                     /translation="MTPKLAWIISTVVVIAAFVAINSWAAVPKHESNSAAPIAAKGAI
                     YRPDLWKVY"
     gene            complement(5735..5878)
                     /locus_tag="VM910_21425"
     CDS             complement(5735..5878)
                     /locus_tag="VM910_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65119.1"
                     /translation="MEQRWLALARSYEFAERLSRFIVPFSKGKQPKNSRQRRLDEKEP
                     PRP"
     gene            5892..6143
                     /locus_tag="VM910_21430"
     CDS             5892..6143
                     /locus_tag="VM910_21430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65120.1"
                     /translation="MIANAPPRARASSPLEERRGLTASLRARSGRTYIDRILRGEKPG
                     NLPIQVPTRYELVINMKAAKVIGLTIPPAFPLRADEVIE"
     gene            complement(7341..7598)
                     /locus_tag="VM910_21435"
     CDS             complement(7341..7598)
                     /locus_tag="VM910_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65121.1"
                     /translation="MPEVLNEVDHSESAEFVPSSRIALGVSVSQKITPCPSALADRER
                     LYELRAQQSLTAAFFGDPLPGRSALDQRRAEQERIARQSEA"
     gene            7707..7964
                     /locus_tag="VM910_21440"
     CDS             7707..7964
                     /locus_tag="VM910_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65122.1"
                     /translation="MDKDYLILKGASASRPSGEWNDDDFDVLCDGVVVGRIMKAAAVP
                     VDMSWMWTLAFGYHEDRTPTHGYAAMREAAMAAFAKSWRQE"
BASE COUNT         1637 a         2621 c         2741 g         1709 t
ORIGIN      
        1 ctcttgcgga caggccgagg tgaccggagg aggagcggcg ggcttgagcg gggggcgaac
       61 ggagagccga tcgctcttgg cagcggccgg ggcggcggga tcggcgttcg cctcggaatt
      121 ggcggcgacg gcggcggccg cagcggggtc ggtctttacc tccggattgg cggcaagcgc
      181 cgtcaccacg gcagcgccga ttgcaagcgc ggtcacgcca gctgcgagct tggagacgga
      241 catttgacca cccacccagc cgcccccgtt gcgcgaagac gcgctcgggg gttctgggag
      301 ttagtcgcgg tggcgaaaca aaaggttcac gcttgaaagc gcgttttccc gcccattttt
      361 cggtagtggg ccagtttcat tggggcaccc cctcaccgag ggtcgcgatt tcaaaggcat
      421 agtagcccca ggcgcggtgg ttgctgaggc ccgagcctgg ggccgggcat ccctcctagg
      481 aagccccggg aggtcgccca agggcgatta tcgtaatgtt ataatgttac acaaggctat
      541 gataggtctt tggcgatgac gttgaggtac gggatcgaat agaggccgcc ctgcccccat
      601 acactggagg cctcgaagcc gagatacctt gcttgttgcg cggcagcttg cgccgcgctc
      661 acaccactcc caactgcgaa tattcgatgc ggcgataaaa gccgacttgc gccgacttgc
      721 ggtcgagcgt aagcgctagg tagcggaacg agtcggccgc gtgcgaggtc cagtcgtgca
      781 ccggctgggc cgcagcgcct gcagcttgtc gtcgtattcg gcacggtaga gcttgagcgc
      841 atcgatcccg cgcgcgcatt tctttgcgtc gaaccagcgt ttcggcagga agacgcgcac
      901 ggcgttgatg ccgtcctcca cccggtgcag cggggcgatg ctgatgttct tcaggcccaa
      961 gctttggagc acctcgagcc gactcttgcc ggtgcccagc tccttggcct gggcgtcgtg
     1021 cggcacgatg tgcccggcat aaacgtaagg ccgcgcaccg atctccctga cgtagtggcc
     1081 gagatcgacg ccggaggctt cgtagtagtc gatgatcctg atctcgcgcc cgatcacctg
     1141 cgcaaaccag atcgcggtcg catcgcggat gccgaggtcc caggacgtcc acacgagcgc
     1201 ggccggatca tagggcacgg gcgcgatgcg ctgatcctgc tcggcgcgcg ccatcagctt
     1261 cccgtagtag gagccgacga tcgcggcgtc gaacgagcat tcgaactctt gcgcatattg
     1321 gtcctcgctg agatcgcgcc tggcgagcgc gagctcgctt tcggcaatga ggccggtctc
     1381 gcttgccttg agcatcaggg gaaaaccaat gctgttccga ctgcgagcgc cgccacagct
     1441 cgaagaaggc gttgcggccc ttgggcgtgc cgatgaagac cgcccagccc tcccgatcgg
     1501 ccaacgccgg ccggatgatc tccgaccaca cgcgcgggtc catgtcggcg tattcgtcga
     1561 gcacgatacc gtcgagatag atgccgcgca acgcatcggg attgtcggcg ccatagagcc
     1621 gcacctgccc tccgttggga taatcgacgc gcagctcggt ttcgtgcgcg ctcgccccga
     1681 tcgcccgcac cggcgccatg gcgttgcgca gatagtccca cgccaccgcc ttcgattgct
     1741 tgaggaaggg cgagagatag gcgaagcgcg ggcgtatacg caggcactcg gttgccgcgc
     1801 gctgcaagtc gtgcacgcca ccgtcttgcc ggcgcggcgg tgcgtcacga tgcaggcgga
     1861 gcgctcggtc ctattgtggg aggtgatgaa ctgagtgcgc ggtgcgtaat cgacgacgag
     1921 ctcgggtgct gacatgggag cgtgcttaac caacctgcgt aggatgggtt aggagtcaaa
     1981 gcgcgtcgaa gacgcgctga cggcgccggc accccaccct cgactggcgt tgcgaattga
     2041 ttggtgggtt acgcgcctcc cgcgctaacc tacctacgat tggctggcat cacagtgccg
     2101 gccgtcgtcc cggcgcgggg caccccgcga tgctggcgtc gcgggggccg catcttcggc
     2161 gttcagcggc cgcggatcgt ttcggttttg acgcggacgc gcggcattgc gttggctgcc
     2221 gccgtgtaca cgtgcaggca ggtgctgggg caagcatcgc gcgcgctgcc ggcgagcgcg
     2281 gggccgagaa tgtgtttgcg ctcggcccgc tcgcgaacga gcagatcgac gaggctttgc
     2341 tcttcgagcg gcagcgcagg atcgaaatag cgatcgagca ccgggacgta aacgagaacg
     2401 cggtcgatcg gatcggccgg cgccattgtc gctgaaacga gctcggcatc gatcccattg
     2461 tcgcgcagca ggttgatcag caagaccgcg gacacgctgc agtcgtcgcg cttcccctcg
     2521 gcggcgggcg agttcgcggg ctgccgccgc aacccggcaa aggccgcaag tagcgcgttt
     2581 gcgtcggatg tgcgcgagat gccgagcgcc tcggcctgct gccggcatgc atcggagaaa
     2641 aaggccgcgc gaatgagctg cgtgtcgact atggtcgcag ccagccccgg agccgtcatc
     2701 gcaaggcaaa gggcgagaac cgcaaagatc ttcagaccca tgaatttacc tttcaagcta
     2761 cggaacgagc ccaggacagc ctcgcctgcg gatcatagca caaacagatg ccctgtgcct
     2821 tgagcctagc gaaactgttg cggcaagatg ggagcgccct cctgtgggtt tattggcaca
     2881 ggttcgaaca acttcgaact cggaatagga gtggaatcgg acctaaagaa cacgtttgtc
     2941 gatagtggcc gggggtcgaa cagcaaatgg cgccaaagat gctcattcac tagcacccct
     3001 aacggagatc gagaaaagtc aaacggattc gtcaatgtga caggtgcgtc tttgactggc
     3061 gcatcacggc taattgctgt taccgcaaag ccgggctgga gcggcgggac acgatcattg
     3121 aaatcgaaaa tgctgaatag attcccggcg cggcctaaat tgtcaggcaa cacgccatgt
     3181 cgaggggaac gatagtagtt tccgttcgtc attggaacct cccgtggata ggggacacac
     3241 gccaccgagg ggcggtggcg cgggcgatca ttccgccgat gccccgtcgc agacacggcc
     3301 gcaccgaggc ggtgattcac gcgtagtttt ggtgaatatg cttttttgga agattctcgt
     3361 tacgcgagtc aaatgaaaaa tgtctcagtt tgaaattcta tcgtttcgct atgagcttat
     3421 aagcagggca gcctcccgaa ggcccgcgag caaccaacgt ggatgactca gcccccggcc
     3481 ccgcctcgat cgccgctctg cgcgacgccg tgcggaacgg ccgcgagccg ccgccggctt
     3541 ggatcgtggc gcgccttaac tcctaccggt ggtgcgtcgt cggcaccgtg tgcgtcggcg
     3601 ccttcatggg ccaggtcgat tccagcattg cacagatgct gctcccgcgg ctcgagctcg
     3661 aattcggcgc aaggctcagc accgtgagct gggtcgcggt cgcctatctc ttggcaatgg
     3721 cggcgttcct gccgatcttc ggtcggctcg ccgacatggt agggcgcaag ctcctgtaca
     3781 cgggcggctt cctcctgttc gtgctgagct cggcgctctg cggcctggct cccagcctgc
     3841 ccgtgctcat cgccttccgg gtcgcacagg gcatcggtgc ggcgctgctg tcgtcgaaca
     3901 gcgtcgcgat tgtggtgacg gcggccggac ccgcgcagcg cggacgcgca ctcggcgtcc
     3961 tgtcggcagc ccaagccgtc ggattaagcg ccggcccggc cctcggcggc ctcgttctgg
     4021 acctgctgga ctggcgatgg gtgttctgga tcaacgtccc gatcggcctc gccggcacgg
     4081 tcctgggctg gttcgtgctt ccgcccaccc aggacttgcc cgctgacagc cgtttcgact
     4141 ggaagggcgc attcctgatc gcgcccgcgc tcaccgcatt cgtggccgtg ctccacgaag
     4201 ggtacgcatg gggggcgacg tcgcccgccc ttctcggctg tgcgctgctg gcggtgatcc
     4261 tgctgacgct gttcgttcgc gccgagcaac gcgcggatgc gccgctgatc gacctcacgt
     4321 tattccggca aagcgcgttc tcggccggca atgtcgccgg cctcatgggc tacgcggcgc
     4381 tgttcggcct gttctttttg atgcccttca tcttcgtgcg cgcctaccgg gattcgacgt
     4441 tcgccgcggg tctgcggctt tccatcgtcc cggtgatgct tggcatggtc gcgccgatcg
     4501 ccggcgcact ctacgaccgg ctcggccccc ggctcctgac cgtatcgggc atgctcatct
     4561 gcgtcgtcgc attgctgttg ctcttcgccg tgctggacgg cgcgcccgca agcctgccgc
     4621 tcgtgatgct cgccctcgcg gttttcggaa tcggccaggg gttgttcatc tcgcccaaca
     4681 acagcgcggt cgtggccgcc gcgccagcgt ctctgaccgg ggaagccggg ggactcctga
     4741 acgtgatgcg ctcctgcggc atcagtattg gcgttgcggc cgcctcctcg ctgctcgctt
     4801 ggcggctcgc ggtccgcacg ggctctggcc acaacactct ccacgccgaa acgcgccacc
     4861 tgttggcagc gggccgcgac gtgattgtcc tcatcgggag ctacgctgcc atggcgggcg
     4921 cgatttcgct cgcacgactg ccctcacggc gacccggcaa tgcaaaggat gcatcggcct
     4981 aaggtctatt tgaagcgctc atccggcctg aaccgcgctc gcattcaatt tcgaggctaa
     5041 cccagcgttt tgcttattag tctccgtaca aatgcccgca taattcatcg agcgcgcctt
     5101 tgcacaagag atcgggattg cgctcgccgg ccttggcaag ttggacaatt cgtctggcga
     5161 tgattccgtc ggcgaaattg tctccaagcc caagggatac gcgagtcatt tcgagcgcga
     5221 cacctagaac ccgcttggtt tcgggactgg catcgaagtc gcctaggtaa ggcgtgatgg
     5281 acatgcctac ctccgacaat atcttggcaa aaagccatta gcgtgcaact ttattatctt
     5341 cattgaaata ataagcgacg tttatagacg cgcgccctag cgcacctcgc gggcctcttt
     5401 gcgcgtcgcg gcataaccgg gcgtcggcgt acggcccggc cgcggctata gcttccgcct
     5461 atacgaccaa agcgtgacgg gcgtcacaga tttctttccg attccccctg taggagtgct
     5521 gctagaagcg cgaggagagg aagacatgac accgaaactc gcatggatca tttccacggt
     5581 tgttgtgatc gcggcattcg tggcgattaa ctcatgggcg gctgtcccga aacatgagag
     5641 caattcggct gcgcccatcg cagccaaggg agcgatttac cggcccgacc tgtggaaggt
     5701 gtactaggtc cgcactcgcc gaccgggcgg gggcttatgg acgcggcggt tctttttcat
     5761 caagtcgccg ctgccgacta ttcttcggct gcttgccctt gctgaagggc acgatgaacc
     5821 tcgacagcct ttctgcaaat tcatagctgc gggccagagc cagccagcgc tgctccattt
     5881 cgagataatc attgatcgca aatgcgccgc ctcgggccag ggccagttcc ccgctcgaag
     5941 aacgccgcgg tctgacggcc tccctccggg cccgatcagg tcgaacctac atcgatcgca
     6001 tcttgcgtgg cgagaagccg ggcaacctgc cgattcaggt gccaaccaga tacgagctgg
     6061 tgatcaatat gaaggctgcc aaggtgatcg gattgaccat cccgcccgca tttccgctgc
     6121 gcgccgacga ggtgatcgaa tagcgacgca ttttgctgcg gtgcatgagt ccgcttttgg
     6181 cacaaagcgg acatgtcgag atgtctcggt ccggcggtga aacgcgaagc tgaagaaggc
     6241 tggagatggc ctcaagcttt tttgacttgt gtggccgtaa aagcacagtc cacgacaact
     6301 gctcagagta tctttagggc tagctccagc ggggatcggg gaaagcctgc tgcagccgag
     6361 agagcccctg gagttgtccc cttcggattt cctccagggg ctctcgacca cggcgctggc
     6421 gtatcccgcg cagcgaccgt accccgaggc cacagatgct cagcccgatt ggtgccttcc
     6481 ttcttgccct aaacctattc accgtgacta ccactgtggt gttcgctcag acaggctcca
     6541 cggctacgca gggcaattca accgcagcca atcgcactga aattcatctt cacaattacg
     6601 gtgagttcaa taaggcgtgt gtggcatgga ctgatggttg tcgcaactgc agccgcgact
     6661 cgggttgctc gaatatcgga atcgcgtgcc aaccaacgga ggtgacgtgc ctgcaacacc
     6721 tacccttgga gaaaaacgag cgatagcacg gcgacgcggc gacccatgag tcagcaatat
     6781 gtggattgtg ctagttcgct tgttcaccat tctcttcgtg ttgactctcg cagcctgcga
     6841 aaggaaagag gtcgcctctc aagaagaaaa gggtagcgag gagccccctg gtccagccag
     6901 gtctcctggc cttcctggcc ctgccggctc cccaggagga acggccattc gcttcgtcga
     6961 tggagaatgt cgccagatct gcactgttgc atgcgaggct aacgaacgta tcctcaatgc
     7021 gtttgcccta ggcccgagcg gcagttttgt ttttgaagac gagaatcggg cgacgttccg
     7081 gccacagcga cagggcgccc cggtcagggt ggttctcgcg tgtgctcaca agtgaacatt
     7141 ctggcgagtc ctgatccccg agagcctttg acgtgcccgc ggtcaaacga ggccgagggg
     7201 gattagggaa aggagcgctg gcgataaggc gattctcaga gtaggcttgt ggccgcgatt
     7261 acaatccccg taacgaagac gacgaaacag gagaatagca gcacgttttc gacactgatg
     7321 gtcatagagt cccccgatgt ctaagcctcg ctctgacggg cgattcgctc ttgttcagct
     7381 cgccgctgat cgagcgccga tctacccggc agaggatcgc caaaaaaagc tgccgtcagg
     7441 ctttgctgcg ctcgcaactc gtacaggcgc tcacgatccg ctaatgctga agggcacggc
     7501 gtaatctttt gcgagaccga aacgcccaga gcgatgcgtg atgatggcac gaattcagcg
     7561 gactcgctgt gatcgacttc attcaaaact tccggcattg agattccccc ctacggcctc
     7621 cccaggctgc agccctaggg aacgccacag catcttggca aaacgctgac ggtgagctgt
     7681 ctggattaag acaatgtggg gtgccaatgg acaaggatta tctgatcctc aagggcgcct
     7741 cagcgagccg cccgtccggc gagtggaatg atgacgattt cgacgtgctc tgcgatggcg
     7801 tcgtggtcgg ccggatcatg aaggcggccg ctgtgccggt ggacatgtcg tggatgtgga
     7861 cgctggcttt tggttatcat gaggatcgca cgccaacaca cggctacgcg gcgatgcgtg
     7921 aggccgcaat ggctgcattc gcaaaaagtt ggcggcagga ataaggcttc gagaaatgcg
     7981 caaaaatgtg atggccgtca catcctgaca gcgggcgatg tgaaatcatc ccaactaagg
     8041 cgatatggac ggccccgagc agctcccccg aatcccccag ggttgtccaa atacgccgaa
     8101 agaaggtgtc gctagccccg cttcggcggg gctttttctt tgagcctccg aggacacccc
     8161 gtgagcgtgg tgcttgcgcg gcaaagaaag ccccgctgga cgcgcggggc tggctggcgc
     8221 aggtgcgatt gggctcagag caaatatttg gcgcaaagta acaagtcttg gacggggcaa
     8281 agcctgatct ttgactagat agagttgcaa tcaccgaggt gattccgcag gttagagcga
     8341 accagtgtcc ttttcagacg attatttcgt agtcgctaaa ccccgagggc acccgcggtc
     8401 atgggcgtgg gaaattcgga ggcgatcaaa gccaattgga gtcagactta gcggcgacgg
     8461 tttcaaatcg gagtttgcag cgaaactcgc cggtgaaaag gcactgagag agcttctaga
     8521 tggccttgcc caagaggaca atgtttgagc gctgcaaggc cgaaaagaaa tacagttagg
     8581 acttcactca tgccgccacg tattgacgtg gcccgtatgc cctattggtt gccttatagt
     8641 cgggcggtgg cgttgccaac aaaaccagcg gtttgcacaa actattgcat tgtgtaacga
     8701 cgcgggag
//
LOCUS       DATLJT010000938         4264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4198122,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000938 DATLJT010000000
VERSION     DATLJT010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4198122"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VM910_21445"
     CDS             <1..396
                     /locus_tag="VM910_21445"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI65123.1"
                     /translation="MADIDPNRIFLQGYSWGAVSSLFAVDTKNPVKHHTKIAGVIAYY
                     PRCYEGVDPSVPTLVMIGDQDDWTPVRACQAVAGKPNLEVVVYPNQTHGFVLPGEDGY
                     FLGHHMVYDEKATRDAEQRADGFMAAHMK"
     gene            complement(449..2038)
                     /locus_tag="VM910_21450"
     CDS             complement(449..2038)
                     /locus_tag="VM910_21450"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVI65124.1"
                     /translation="MPDQFSKLQQIKKPAVRSRGGIVASQSRKAAEIGAGVLAAGGDC
                     VDAAIATGFALGVLEPWMSGIGGGGAMVLYRAREDRVEVIDYGMRAPLALRPEDYPIV
                     QGAASDIFPWPRVQDDRNLVGPSSIAVPGVVAGHEEAHRRYAKMPWRELLSPAVKLAG
                     EGLLVDWWTTLAIAGSAAELRRYPASGEAYLVDGLPPNPQWGIRSAVRLLQAKLKATL
                     DHLATQGPRDFYEGDLAKSVVADVHSVGGVLSTSDLAAFRPHLRQPLQIPYRGDIVYA
                     TPELTAGPTLAHALRLLQHDFKPASGTPDASAYAAYALALQAAYRERLKDMGDTDGRR
                     ALGAEDLAPACTTHYSVVDRDGNMAAVTQTLLSTFGSKFVTPRTGITMNNGIMWFDPT
                     PGTPNSLGPGKRCLTNYTPVVARTADGRRLALGASGGRRILPCVTQILSFIMDFGMDL
                     DAAIHQARLDASEGAIVIADVRLPAAVREDLKERFDYEEAHMQTYPGKFAFPSIVLRE
                     NETNFGATEVFHPWADAVAEG"
     gene            2231..3322
                     /locus_tag="VM910_21455"
     CDS             2231..3322
                     /locus_tag="VM910_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI65125.1"
                     /translation="MTISRRQILTGGAACAATAIAGRASAQSFPFKPNQRYPEPALEI
                     LDPSFLKYRIYSSTVEQLASGMRWAEGPVYFPDGGYLLVSDIPNNRIMKYSEKDSSFT
                     VFRSPSNFANGNTRDRQGRLVTCEHSVTRRITRTEKNGKITVLAENFEGKRLNAPNDI
                     VVKSDDTIWFTDPPFGINGDWEGFKAKPEQANTNVFRIGTDGKLTAVITDLVNPNGLA
                     FSPDEKKLYVVEVRGTPNRSVWSYDVGADGNLSNKTRLIDASDQGSPDGFRVDRDGNL
                     WCGWGSNGLLQTEATEVGGGRKAYQLRGRSEDLDGVKVFNAEGKPIAFIHLPERCANL
                     TFGGPKNNRLYMAASHSLYAYYVESHGAT"
     gene            complement(3364..4107)
                     /locus_tag="VM910_21460"
     CDS             complement(3364..4107)
                     /locus_tag="VM910_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI65126.1"
                     /translation="MDTVRSPVSAAEPVLRRLGALRKLSADGAAALERAIRERILRAA
                     AGEDLITEGDRPASVHMILSGWAYRYKTLQDGRRQIVNFALPGETCDAHLYLLSDMDH
                     SIGALTPVVYAEIERGRFEELVASDRTVAEALWCETLVMNAIQREWAINLGRRDALER
                     VAHLFCEVFERLRCVGLTEGTSCAFPVTQMDMADATGLSVVHLNRTLQELRAARLITL
                     RERNLTINDLDGLRDAGLFNPNYLHLRQR"
BASE COUNT          812 a         1352 c         1336 g          764 t
ORIGIN      
        1 atggctgata tcgatccgaa tagaatcttc ctgcaaggct attcctgggg ggcggtttcg
       61 tcgcttttcg ccgtcgatac aaaaaacccg gtgaaacatc atacaaaaat tgcaggcgtc
      121 atcgcgtact atccgcgttg ctatgaaggc gtcgatccgt cagtcccgac gctggtcatg
      181 atcggtgatc aggacgactg gactcccgtc agggcctgtc aagccgtagc cggcaagccg
      241 aatctcgagg tcgttgtcta tcccaaccag actcatggtt ttgttctgcc gggtgaggat
      301 ggctacttcc tcggtcatca tatggtttac gatgaaaagg cgacgcggga cgcggagcaa
      361 cgcgccgacg gcttcatggc cgcgcacatg aagtaaggac aattgcgtgc gcgtggagag
      421 cgcctgctgg acttcctcgc gccgcaagtc agccttctgc gacggcgtcg gcccaggggt
      481 gaaacacctc ggtggctccg aagttcgttt cattctcgcg cagcacgata ctcgggaagg
      541 cgaacttgcc cggataggtc tgcatgtgcg cctcttcata atcgaagcgc tccttgaggt
      601 cctcgcgcac cgcggccggc aggcgtacat cggcgatcac gatggcgccc tcgctcgcat
      661 cgaggcgggc ctggtgaatc gcggcgtcga ggtccatgcc gaaatccatg atgaaggaaa
      721 ggatctgcgt gacgcagggc aggatgcgcc gtcctccgga agcgccgagc gcgagccgtc
      781 gtccgtcagc agtccgcgcc accacgggcg tgtagttggt gaggcagcgc ttgcccggcc
      841 caagcgagtt gggcgtgccc ggcgtcggat caaaccacat gatgccgttg ttcatagtga
      901 tcccggtccg cggcgtcacg aatttcgagc cgaaggtcga gagcagcgtc tgcgtcaccg
      961 ccgccatgtt gccgtcgcgg tcgacgactg aataatgcgt ggtgcaggcg ggcgcgaggt
     1021 cttcggcgcc gagcgcgcgg cggccatcgg tatcgcccat gtccttgagc cgttcgcgat
     1081 aggcagcttg cagcgcgagc gcataggcgg cgtaggcgga tgcatcgggg gtgccgcttg
     1141 cgggcttaaa gtcatgctgc aaaagccgca gggcgtgtgc aagcgtcggt ccggcggtca
     1201 gttccggcgt cgcgtagacg atgtcgccgc gataggggat ttgcagcggc tgtcgcagat
     1261 gcgggcggaa ggcggcgagg tcgcttgtcg agagcacgcc gcctacggaa tgcacgtcgg
     1321 cgacaacgct cttcgccaga tcgccctcgt agaaatcgcg cggtccctgc gtcgcgagat
     1381 ggtcgagcgt cgccttcagc ttcgcttgaa gcagccgcac cgccgagcgg atgccccatt
     1441 gcggatttgg cggcagtcca tcaacgagat aggcctcgcc gctcgcggga taccgccgca
     1501 gctccgcggc cgagccggcg atcgcaagtg tcgtccacca atcgaccagc agcccttcgc
     1561 ccgcgagctt gacggccggc gaaagcagct cgcgccaagg catcttggcg tagcgtcgat
     1621 gtgcttcctc gtggcctgcg accacgcccg gtacggcaat cgagctgggg ccaacaaggt
     1681 tgcggtcgtc ctgcacccgt ggccagggaa agatgtcgga agcggccccc tgcacgatcg
     1741 gatagtcttc ggggcgcagc gcgagcggcg cgcgcatgcc gtaatcgatc acctcgacgc
     1801 gatcctcgcg cgcgcggtag agcaccatcg cgccaccgcc gccgatcccg ctcatccacg
     1861 gctcgagcac gccgagcgca aagccggtcg caattgccgc atcgacgcag tcgccgcccg
     1921 cggcaagcac cccggcgccg atctcggcgg ccttccgtga ttgcgatgcg acaatgccgc
     1981 ctctggagcg gacggccggc tttttgatct gctggagctt cgaaaactgg tcgggcatga
     2041 tgtttttgat aatctttttc atatcggcgg gttggacgcg cggtaagcct aacacgaatg
     2101 cggaatcgat atgattcggg atcgattcat gcgccaagaa aatttgagcg gcaaaagctg
     2161 cggcaatagg atatccgcga gacaaccagc gccggctttt gcacggcaaa aagacaaggg
     2221 gaggcaagcc atgaccatct cacgcagaca aattctcaca ggcggcgccg cgtgcgcggc
     2281 aacggcgatc gctggccgcg ccagcgcaca gtcctttccg ttcaagccga accagcgcta
     2341 tcctgagccc gcgctcgaaa tcctcgatcc gagctttttg aaataccgca tctactcgag
     2401 cacggtggaa cagctcgcaa gcggcatgcg atgggctgaa ggcccggtct attttccgga
     2461 cggcggatat ctgctcgtca gcgacatccc gaacaaccgc atcatgaaat attcggagaa
     2521 ggacagcagt ttcaccgtct tccgcagtcc ttccaatttc gccaacggga acacgcgcga
     2581 ccgccagggg cgcctcgtca cctgcgagca ttcggtaacg cgccgcatca cgcgtaccga
     2641 gaagaacggc aagatcaccg tgcttgcgga gaattttgaa ggcaagcggc tcaatgcgcc
     2701 gaacgacatc gtggtgaagt cggacgacac gatctggttc accgatccgc ccttcggcat
     2761 caatggcgac tgggaaggct tcaaggccaa acccgaacag gccaacacca acgtgttccg
     2821 gatcggcacc gacggcaagc tgaccgccgt catcaccgat ctcgtcaatc cgaatggcct
     2881 tgccttctcg ccggacgaaa agaagctcta cgtcgtcgag gttcgcggca cgcccaatcg
     2941 cagcgtctgg agctatgacg tcggcgccga cgggaactta agcaacaaga ccaggctgat
     3001 cgatgcgtcc gaccagggtt cgccagacgg tttccgcgtc gatcgcgacg gcaatctgtg
     3061 gtgcggctgg ggcagcaacg gcctgttgca gacggaggcg acggaagttg gcggcggccg
     3121 caaggcctac cagctcaggg gcaggtcgga agatctcgac ggcgtgaagg tgttcaacgc
     3181 ggaaggcaag ccgatcgcct tcattcactt gcccgagcgc tgcgcgaacc tgactttcgg
     3241 cggcccgaag aacaaccgcc tctacatggc ggcgtcgcat tcgctttacg cctattatgt
     3301 cgagtcgcac ggcgcgacct gaggccgttc gagcttgccg gcgagcacgc ggtgcacgcc
     3361 ggtctatcgc tgccgcagat gcagataatt cgggttgaac aggccggcgt cgcgcaagcc
     3421 gtcaaggtca ttgatcgtca agttccgctc tctcagcgtg atcagtcggg ccgcgcgcag
     3481 ctcctgcagc gtacgattga gatgcaccac cgacaggcca gttgcgtcgg ccatatccat
     3541 ctgggtcacc ggaaaggcgc aggaagtgcc ctcggtcagg ccgacgcagc gcagccgctc
     3601 gaagacctcg cagaacaggt gcgcgacccg ctccagcgcg tcgcgccggc cgagattgat
     3661 ggcccactcg cgctggatcg cattcatcac gagcgtctcg caccataacg cttccgccac
     3721 cgtgcgatcg ctcgccacca gttcctcgaa gcggccccgt tcgatctcgg cgtaaaccac
     3781 aggggtcaag gcgccgatcg aatgatccat gtcggagagc agatagagat gcgcgtcgca
     3841 ggtctcgccg ggcagcgcga agttcacgat ctgccgcctg ccgtcctgca gcgtcttgta
     3901 ccggtacgcc catcccgaaa ggatcatgtg aacgcttgcc ggtcggtcgc cttccgtgat
     3961 caggtcctcg ccggcggccg cgcgcaggat ccgctcgcga atggcccttt cgagagcggc
     4021 tgcgccgtct gccgacagct tgcgcagcgc tccgagccgg cgcagtacgg gctcggccgc
     4081 gcttaccggg gatcgcacgg tatccatcag ccgcccatcg ccgacgtgtg agcggcgcgg
     4141 ctcaccggaa tcaccgccgc gatcgctatg cttttcagga aattcttcaa atcgcttgtc
     4201 aggagccttc gtccgcgacg gttttcgccc ttctcttgcg atccgccgct taacataagc
     4261 gccc
//
LOCUS       DATLJT010000939         8387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2820247,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000939 DATLJT010000000
VERSION     DATLJT010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820247"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..782
                     /locus_tag="VM910_21465"
     CDS             156..782
                     /locus_tag="VM910_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI65127.1"
                     /translation="MEKPRVRIYTDYKSPYAFVATERLFALEELGVELEWLPYTLRIA
                     EFMGKVEERTPHFWRKVHYSYMDARRFANAQGLVLKGPRRIYDAFYSSAGMLFAKQHG
                     FFRPYHETVFRRFWSHDLDIDDLPQISAVIASLGGSAREFEGYVRGPARAEHDCIIDE
                     AEALGVFGVPAMVFNGELFWGGDRIDMLIERIRNPEGIAVALGSRHRK"
     gene            complement(793..1803)
                     /locus_tag="VM910_21470"
     CDS             complement(793..1803)
                     /locus_tag="VM910_21470"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI65128.1"
                     /translation="MSEMRYRLIGSTASPYAIKLRALMRYRRIPFDWVIMTKALRKET
                     EQLRPNLIPILQYPDGTFRGETTMLAYDLEARHAERSVIPQDKAVAFVCNLLEDLADE
                     WAVKPLFLYRWWDAEDQAYVSRWAAEEWSTSKAEIGSAAEIEEFRKRQISRMGILGAT
                     AENRPLLQESYRRILAAFEAHVGIGKYLFGSRPSLADFAWFGQLSEMATDPTPMRIMR
                     ERAPFTDHWVRRLDDASGVDGAWSSREQALGKWAEALLQIAGELYLPFLVANAEAFAK
                     GLERLEIRVWGLPYMLAPFKYQVKCLEQLRSNFADLDAENRAALKPILSRTGCWAPLA
                     VQ"
     gene            complement(1860..2903)
                     /locus_tag="VM910_21475"
     CDS             complement(1860..2903)
                     /locus_tag="VM910_21475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HVI65129.1"
                     /translation="MHDDFVRGEPPTHSGTALPGTDQLFRQFAEASLDVLWLRDAKTL
                     QWEYVSPGFEAVYGLSREQALVRNDLSQWADLILSDDRAAALAAIYRAREGERLSFEY
                     RIKRPGDGRIRWLRSNVFPMPDQHGHVQWIGGIGRDISELKSATDHQRRLLAELQHRV
                     RNTLAVIRSIVRRTGESSESVDDFASHLDGRINAFARVQLAATRDPLTGFDLGDLIAE
                     ELRACAAREGEQFELRGPRVRLKPKAAESIGLATHELATNAVKHGAFTAGAGHIAVDW
                     RVEERQEENWLCVDWTESGMRGRPVEQKREGFGTLLLQQTLQYDLGARVNRIFAPDGF
                     RCEMAFPLATVAS"
     gene            complement(2938..3675)
                     /locus_tag="VM910_21480"
     CDS             complement(2938..3675)
                     /locus_tag="VM910_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI65130.1"
                     /translation="MSHLIRKLEHFVRLSATDRAVLDRAASERVRSFGPRVDIAREGD
                     RPRDVHMILAGWACRYKQLEDGRRQIVSFFLPGDICDLNIFILREMDHSIGTITSVTV
                     ADLSREFFDEISSGFPRIATAFWWESLVNAAIQREWTMSLGQRTAAERMSHLLCEIFL
                     RLRLAGLTREESCDFPLTQSDLADATGLSKVHVNRTLQELRSAELIVLKGKSLTIPNL
                     ERLMNEGLFNANYLHMNREGRQLDANE"
     gene            3798..4034
                     /locus_tag="VM910_21485"
     CDS             3798..4034
                     /locus_tag="VM910_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65131.1"
                     /translation="MGRIGQSALVGRRILIVEDEYFLADDLRQILSDQRAEVLGPVAT
                     IANALSVISNTGPIDCAVLDINLGGEAAFPISMH"
     gene            4188..4445
                     /locus_tag="VM910_21490"
     CDS             4188..4445
                     /locus_tag="VM910_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65132.1"
                     /translation="MKLVGKTLASAALGAGLLAASAVSASAAIVCSGNVCWHTHERLA
                     YPPEARVIIHDDDWRWEPHERYTFREHEGRGYWRGGDWVEW"
     gene            complement(4599..4961)
                     /locus_tag="VM910_21495"
     CDS             complement(4599..4961)
                     /locus_tag="VM910_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVI65133.1"
                     /translation="MTTDEREFGNLIGSDKVEGTAVFGAGDRKIGSIERVMIDKISGR
                     VSYAVLGFGGFLGLGNEHYPLPWQSLKYDTDLGGYRTGITEDQLRGAPRYSEEADWNW
                     SDAARNRALNAYYGVPIA"
     gene            5145..5354
                     /locus_tag="VM910_21500"
     CDS             5145..5354
                     /locus_tag="VM910_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65134.1"
                     /translation="MLKNSVQIALNYLERSGEIDDYKETCEFLVDEVASMIRHGQHNK
                     LLLANRAIAAFQHYRTARTIEPVSG"
     gene            complement(5606..5836)
                     /locus_tag="VM910_21505"
     CDS             complement(5606..5836)
                     /locus_tag="VM910_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65135.1"
                     /translation="MPLYHFELVNLETIADQGGAELSDDIEAMDSADALARRILSRRP
                     DLRNHDYAILVTNEDGDEICRLPLDIIHQSLH"
     gene            complement(6015..6152)
                     /locus_tag="VM910_21510"
     CDS             complement(6015..6152)
                     /locus_tag="VM910_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65136.1"
                     /translation="MRARRVLVILLVAFTVASTIAALTTIRQMRTEMSSVSPPGIKIK
                     I"
     gene            complement(6152..6340)
                     /locus_tag="VM910_21515"
     CDS             complement(6152..6340)
                     /locus_tag="VM910_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65137.1"
                     /translation="MKQSDIFRENAENCLQLAEQAEGRPAYRRYVRMARAWTALAREQ
                     QWLDGEISPLPIKTEELA"
     gene            6678..>8387
                     /locus_tag="VM910_21520"
     CDS             6678..>8387
                     /locus_tag="VM910_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVI65138.1"
                     /translation="MSDLDPRPASTPLRPLSKRKANGAPSPDSLQDLLHALQAMRSGD
                     FSVRMTGDQIGIDGKIADTFNEIIAANERMAQQLERVGQVVGREGKTRQRVRFGLASG
                     SWADMENSVNTLIDDLLWPTREVTRAVAAVAQGDLLQTVQLDVDGRPLGGEFLQSANI
                     VNTMIKQLSVFTSEVTRVAREVGTEGKLGGQAQVPEVTGVWKDLTESVNSMANNLTGQ
                     VRNIAEVTIAVANGDLSKKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVAREVGT
                     DGKLGGQAIVPGVAGTWKDLTDSVNAMCGNLTAQVRNIANVTTAVARGDLSRKITVDV
                     RGEILELKDTINTMVDQLNSFASEVTRVAREVGTEGKLGGQAQVPGVAGTWKDLTDSV
                     NFMASNLTAQVRNIADVATAIAGGDLSKKITVNVSGEILQLKETLNTMVDQLNAFASE
                     VTRVAREVGTEGKLGGQANVLGVAGTWKDLTESVNSMASNLTGQVRNIAEVTTAVANG
                     DLSKKITVVVRGEILELKDTINTMVDQLNAFAGEVTRVAREVGTEGKLGGQALVRGVA
                     GTWK"
BASE COUNT         1635 a         2622 c         2534 g         1596 t
ORIGIN      
        1 tctggaccca tccgggcatg acgacctatt accgcaacaa gaacggccgc gtgttttccg
       61 cgatgccatg gcggttcgtg gactattggg cgatgaccca tgacccggat ctcggccagt
      121 atcggctgac cgaggtctag caaaggggaa ccgcgatgga aaagcctcgc gtccgcatct
      181 acacggacta caagagtccc tacgctttcg ttgcgaccga acggctgttc gcgctcgagg
      241 agcttggcgt cgaactcgaa tggctgccgt atacgctgcg catcgccgag ttcatgggca
      301 aagtggaaga acgcacgccg catttctggc gcaaggtgca ctattcctat atggacgcgc
      361 ggcgcttcgc caatgcgcag ggactcgtcc tgaaagggcc gcggcgcatc tatgatgcct
      421 tctattccag cgccggcatg ctgtttgcga aacaacacgg cttcttccgg ccgtatcatg
      481 agacggtatt ccggcggttc tggagccacg atctcgacat cgacgacttg ccgcagatat
      541 ccgcggtgat tgcctcgctc ggcggatcgg cgcgggagtt cgaaggctat gtccgtggtc
      601 ctgcacgcgc ggaacacgac tgcatcatcg acgaagccga ggcactcggc gtgttcggcg
      661 tacccgccat ggtgttcaat ggcgaactgt tctggggcgg cgaccgcatc gacatgctga
      721 tcgagcggat ccgaaacccg gaggggattg cggttgcgct cggcagccgc caccggaaat
      781 agcgtcgaac gactactgca cagcgagcgg cgcccagcat ccggtccgcg acaggatcgg
      841 cttgagcgcg gcccgatttt ctgcatcaag atcggcgaaa ttgctgcgga gctgctcgag
      901 gcacttcacc tggtacttga acggggccag catgtaaggc aggccccaga cccgaatctc
      961 gagccgttca aggcctttgg caaaggcttc tgcattggcg acgaggaacg gcagatagag
     1021 ttcgcccgca atctgaagca gcgcctcggc ccatttgccg agtgcctgct cgcgcgaaga
     1081 ccatgcgccg tcgacgcccg aggcatcgtc cagccgccgc acccaatgat cggtgaatgg
     1141 cgcccgttcc cgcatgatcc gcatcggcgt cggatcggtc gccatttcgc tgagctggcc
     1201 gaaccaggca aaatccgcca gcgagggccg gctgccgaac agatatttcc cgatcccgac
     1261 atgcgcctcg aaagcagcaa ggatgcggcg atagctctct tgcaggagcg gccggttttc
     1321 tgcggtcgcg cccaggatgc ccatgcgcga aatctggcgc ttgcgaaatt cttcgatttc
     1381 cgcggcgctg ccgatctcgg ccttcgaggt cgaccactcc tcagccgccc agcgcgagac
     1441 ataggcctga tcctccgcat cccaccaccg ataaagaaac aacggtttga cggcccattc
     1501 gtcggcgagg tcttcgagca gattgcaaac gaaggcgacg gccttgtcct gcggaatcac
     1561 ggagcgctcc gcgtgccgcg cctcgagatc ataggccagc atcgtggtct cgccgcggaa
     1621 agtgccgtcg ggatattgca ggatcgggat caggttgggg cgaagctgct cggtttcctt
     1681 gcgcagcgcc ttggtcatga tgacccagtc gaaggggatg cggcggtagc gcatcaaggc
     1741 gcgcagcttg atcgcatagg gagatgctgt cgatccgatg agcctgtagc gcatttcgct
     1801 cattccattc gcctctggct tcagcgccgc ggcggctgat ccaagcgaag ctgcggggcc
     1861 tatgacgcga ccgtcgcgag cggaaacgcc atctcgcaac gaaaaccgtc cggcgcgaag
     1921 atccggttga cccgtgcgcc gagatcatat tgcagcgttt gctgcagaag gagcgttcca
     1981 aagccttcgc gcttctgttc gaccggacgc cccctcatcc cgctttccgt ccagtcgacg
     2041 cagagccagt tctcctcctg cctttcctca acacgccagt ccaccgcaat atgacctgcg
     2101 ccggcggtga aggcgccgtg cttcaccgca ttggtggcaa gttcatgcgt agcaagcccg
     2161 atgctttcgg ccgccttggg ttttagtcgc acgcggggac cgcgaagctc gaactgctcg
     2221 ccctcacggg cggcgcaggc gcgcaactcc tccgcgatca gatcgccgag atcgaagcct
     2281 gtgagcggat cgcgggtcgc cgcgagttgc acccgcgcga aggcgttgat ccggccgtcg
     2341 agatggctgg caaaatcatc gacggactcg ctgctttccc cggtgcggcg aacgatcgag
     2401 cggatcaccg ccagcgtgtt tcgcacccga tgctgcaatt cggcgagtag tcgcctttga
     2461 tgatccgtcg ccgatttgag ctcgctgatg tcgcgaccga taccgccgat ccattgcacg
     2521 tggccgtgtt gatcgggcat cgggaagacg ttgctgcgca gccacctgat ccttccgtcc
     2581 ccgggccgct tgatcctgta ctcgaagctc aatcgttcgc cttcgcgcgc gcgatagatg
     2641 gcagccagcg cagcggcacg atcgtcactc aggatcaggt cagcccactg agacaggtcg
     2701 tttcgcacga gcgcctgctc acgcgagagc ccgtagaccg cctcgaagcc ggggctgaca
     2761 tattcccact gcaacgtctt cgcgtcccga agccacagga cgtccagcga cgcctcggca
     2821 aactgacgaa acagctgatc ggttccgggc agcgccgtgc cggagtgcgt tggaggctcc
     2881 ccgcgaacga agtcatcgtg catgacagat ccccattcgg aggctatggg acttgagcta
     2941 ttcattggca tcgagctggc ggccctcgcg attcatgtgc aggtagttgg cattgaacaa
     3001 tccctcgttc atcagccgtt ccagattggg aatcgtgagg gatttgcctt tcaggacaat
     3061 cagctcggcg gaccgcagtt cttgcagcgt gcgattgacg tgaaccttgg acagccccgt
     3121 cgcatcggcc agatcggact gcgtcagcgg aaagtcgcag ctctcctccc gagtgagacc
     3181 tgccaggcgt aacctgagaa agatttcgca gagtaaatgc gacatccgct cggcggccgt
     3241 gcgctggccc aggctcatgg tccattcgcg ctggatcgcc gcattcacca ggctttccca
     3301 ccagaatgca gtggcgatac ggggaaagcc cgagctgatc tcgtcgaaga attcgcgcga
     3361 cagatcggcg accgtgactg acgtgatggt gccgatggaa tgatccattt cgcgcaggat
     3421 gaaaatgttg aggtcgcaga tatcgccggg cagaaagaac gacacgatct gacgcctgcc
     3481 atcctcgagc tgcttgtaac gacatgccca accggcgagg atcatatgca catcccttgg
     3541 cctgtcgcct tcgcgggcga tgtccacccg cggaccgaag cttcgcaccc gctcggatgc
     3601 cgcgcgatca agcacggcac gatccgtcgc cgacagcctg acaaagtgct cgagctttcg
     3661 gatgaggtga ctcaacgatc gcgggcctcc ccaatcccta atcgtgcggc cgtcaagcct
     3721 ccttcaataa cctatgttat gtttctcatc actgacatcg cctacccaca aaattaatcg
     3781 caagcctcga gggccagatg gggcggattg gccaaagcgc gcttgtcggg cgccgaatcc
     3841 tgatcgtcga ggacgaatat tttcttgccg atgatttgag acagattctc agcgatcagc
     3901 gcgccgaagt cctcgggcct gtagcgacga tcgccaatgc gctaagcgtg atctccaata
     3961 ccggaccgat cgattgcgcc gtgctcgaca tcaatctggg cggcgaggcc gcattcccga
     4021 tttcgatgca ttgatccggt gaagggtccc gttcctttcg ccaccggcta cgacagcatg
     4081 tacgagcaat gcgcccctgg ttccttttga tccacgtgtt cccgctggaa cggaagccca
     4141 cgttgtccgt tggcccgcgg gcaatgtttg aatggaagga ggacaccatg aagttagtgg
     4201 gcaagaccct cgcaagtgcg gcgcttggcg ccggtctgct ggcggcgtcc gccgtgagcg
     4261 catcggcggc catcgtttgc tccggcaacg tctgctggca cacgcatgag aggctcgctt
     4321 atccgcccga ggcgagagtc atcattcacg acgacgattg gcgatgggag ccgcatgaac
     4381 gctacacgtt ccgtgagcac gagggccgcg gctactggcg cggcggcgat tgggtcgaat
     4441 ggtagctcac gccgacaagg agaggccgcc gtttcggcgg cctctttttt tgatcagcat
     4501 gtgctcccgt gaaaaagaaa acggccctgg ccgcgaacgc gccatggcgt caccggacga
     4561 gaacacgaca aatctttcgt cgctcctctc gaaatgtctc aagcgatcgg cacgccgtaa
     4621 taggcgttga gcgcgcggtt gcgagctgca tcgctccaat tccaatcggc ttcctcgcta
     4681 tacctgggag caccgcgaag ctggtcttcg gtaatgccgg tccgatagcc gccgagatcg
     4741 gtgtcgtatt tcagcgattg ccaaggcagc ggataatgtt cgttgccaag tcccaaaaat
     4801 ccaccgaagc cgagcacggc gtaggatact cgcccgctga tcttgtcgat catgacgcgt
     4861 tcgatcgagc cgatcttgcg atcgcccgcg ccgaaaaccg cggtcccttc gactttatcg
     4921 ctgccgatca gattgccgaa ttccctctcg tctgtcgtca tttgccaaat cctccatgag
     4981 ggccgctcaa agggggcaac gtgttgcaaa agtgatcgtt cccgcgacca gccaccgcat
     5041 cggccgcgca cgcgacttag gaactcccgc gaatgcccgg cgttcgcctt gtgtgcagtg
     5101 aaggatgagt catgtccggt tgttttggtg aaggagcccc gctcttgctg aaaaactcgg
     5161 ttcagatcgc tctgaactat ctcgagcgtt ctggagagat cgacgattat aaggagacgt
     5221 gcgaattcct ggtggacgag gtcgcgtcca tgatccgaca cggtcagcac aacaagcttc
     5281 ttttggccaa tcgtgcgatc gcagcgttcc agcattatcg aacggcgcga accatcgagc
     5341 ccgtttccgg ctagcgcatc gtcatctccg cgatcccccg ggggatcaac gatggtctcg
     5401 tttattcgcg cggttttgcc aatccttttt cgacgccctc gcccgctccg cttcggtaag
     5461 cttcggatca cgcgtctcct ctttcgaggc cgacttgtct ggatctgccc ggggcgccgt
     5521 tcgcgcgcgc tccttgtcgg ccggctgctc ttcggctttc ccatccctga caatgccatg
     5581 atccgaatgc ttcattcgtt gtcctctagt gaaggctctg atgaatgatg tcgagcggca
     5641 ggcggcaaat ttcgtcaccg tcctcgttgg tgaccagaat ggcgtaatcg tgattcctca
     5701 ggtcgggccg ccggctcagg atgcggcgcg ctagtgcgtc ggcggaatcc atcgcctcga
     5761 tgtcgtcgga caattcggcg ccgccttgat ccgcgatcgt ctccaggttc accagttcga
     5821 aatgatagag cggcattttt gatctcctga catagcggga catgagccct gcattgctgc
     5881 agggcggcct tctcggaacc gctactgctg ctacgaaagc agcaggatgt accctggcct
     5941 acgttcggcg gcggcaaatg ttcccaaatc gggggcgacg ccggccgaaa tcggacggcc
     6001 ggccgataac gcgactagat cttgatcttg atgccgggcg gcgagacgga cgacatttcc
     6061 gtccgcatct gcctgatcgt ggtcagcgcc gcgatcgtgg acgccacggt gaacgcaacc
     6121 agaaggataa cgaggacgcg ccgtgctctc atcatgcaag ctcctcggtt ttgatcggta
     6181 gcggggaaat ctccccatcc agccattgct gttcgcgggc gagagccgtc cacgccctgg
     6241 ccatgcgcac atagcgccga tacgccggcc ggccttcggc ctgctccgca agctgcaggc
     6301 agttctccgc gttttcccga aagatgtcgg attgcttcat cgacaggacg tagtgaagaa
     6361 caccgaaaac acaacgatgc gttaagcgat gttaatgcgt ccgccagcgg gcggggttcc
     6421 gggagggcca cctctccgga ttcgttccag cgcggaactg cctttgcatc gcaccccaat
     6481 ttgcatcgca ccgcaaaaag tccgggaagc tcgggaacca aacggccgca ccgaagttga
     6541 agcgcttagg ccaaggcacg cggggtccaa ttcaaagtca tcacgtcttt gaacttgatc
     6601 tgtttttttg acgaagcttc tatggctctg cctcgtgccg agctgatacc gctttgcacc
     6661 ctgaaggggg gaaattgatg agcgatctcg atccccgacc ggcttcaacc cctcttcgtc
     6721 ctctctcaaa acgcaaggcc aacggcgcgc cgagcccgga ctcgctgcag gatttgctgc
     6781 acgcccttca ggcgatgcgc agcggcgact tctccgtccg aatgaccggt gaccagatcg
     6841 gcatcgatgg caagatcgcc gacacgttca atgaaatcat tgcggctaac gagcgcatgg
     6901 cgcagcagct cgagcgcgtc ggccaggtcg tcggccgaga aggaaagacg cgacagcgcg
     6961 tcaggtttgg cctcgccagc gggtcatggg ccgacatgga gaactcggtc aacacgctga
     7021 tcgacgatct gctctggccg actcgcgagg tcacgcgcgc cgttgccgcc gtcgcgcagg
     7081 gggacttgct gcagacggtt cagctcgacg tcgacggccg accgctcggc ggcgaattcc
     7141 tgcagtcggc caacatcgtc aacaccatga tcaagcagct ttcggtcttc acctccgagg
     7201 tgacgcgcgt ggcgcgcgag gtcggtaccg aaggcaagct cggcggacag gctcaggtgc
     7261 ccgaagtgac gggcgtctgg aaggatctga ccgagagcgt caactcgatg gccaacaacc
     7321 tgaccggcca ggttcgcaac atcgccgaag tgacgatcgc ggtcgccaac ggcgacctgt
     7381 cgaagaagat caccgtcgac gtgcgcggcg agatcctgca gctcaaggaa gccatcaaca
     7441 cgatggtcga tcagctgcga tcctttgcct ccgaagtcac gcgtgtggcg cgcgaggtcg
     7501 gcaccgacgg caagctcggt ggtcaggcga tcgtccccgg cgttgccggt acctggaagg
     7561 acctgaccga ctccgtgaac gcgatgtgcg gcaacctgac cgcgcaggtg cgcaacatcg
     7621 ccaacgtcac cactgcggtc gcccgcggcg acctctcgcg caagatcacc gtcgacgtgc
     7681 gcggcgaaat cctggagctg aaggacacca tcaacacgat ggtcgatcag ctcaactcgt
     7741 tcgcatccga agtcacccgc gtcgcccgcg aggtcggcac cgaaggcaag ctcggcggac
     7801 aggcgcaggt gcccggcgtc gccggcacct ggaaggacct gaccgacagc gtcaacttca
     7861 tggcatcgaa cctgaccgct caggtccgca acatcgcgga cgttgcgacc gcgatcgccg
     7921 gcggcgacct ttccaagaag atcacggtga acgtttcggg cgaaatcctg caattgaaag
     7981 aaacgctgaa taccatggtc gaccagctca acgccttcgc gtccgaggtg acgcgcgtgg
     8041 cgcgcgaggt cggcaccgaa ggcaagctcg gcggacaggc caacgtgctc ggcgtcgccg
     8101 gcacctggaa ggacctgacc gagagcgtga actcgatggc gtccaacctg accggccagg
     8161 tgcgcaacat cgccgaggtg acgaccgcgg tcgccaacgg cgacctgtcg aagaagatca
     8221 cggtcgtcgt gcgcggtgaa atcctggagc tgaaggacac catcaacacc atggtcgacc
     8281 agctcaacgc gttcgcgggc gaagtgacgc gcgtcgcccg cgaggtcggc accgaaggaa
     8341 agctcggcgg acaggcgctg gtgcgcggcg tcgctggcac ctggaag
//
LOCUS       DATLJT010000940         4043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_63009,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000940 DATLJT010000000
VERSION     DATLJT010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63009"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..604)
                     /locus_tag="VM910_21525"
     CDS             complement(230..604)
                     /locus_tag="VM910_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI65139.1"
                     /translation="MIGAAVLVASAGSALAQDLTAGEASFRKCQPCHDVGEAAKNKLG
                     PELNGLDGRKAGTVENGTYSQGMRMSGITWNEQAFREFVNNPAAKVPGTTMLLSVRDE
                     KEIGDLWAYLKQFGAEGRKRSQ"
     gene            complement(762..1316)
                     /locus_tag="VM910_21530"
     CDS             complement(762..1316)
                     /locus_tag="VM910_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65140.1"
                     /translation="MMKRALMFVIAFGLAVLGSSSAAWSEDADQAALIKALSGAKHSL
                     LQGIAQVAKGTEVPIEAKYEMEDGKLMLSIYTSAKGFDTAAEDNSFNEYIGDATTTTW
                     APKKEVFADLKHIARSAQYHALLSMTKTTLPTIIQKASAQGTVLSVKEKIRSGKPVFE
                     VMVVQGSTIKPTFYDLVSGQPTAS"
     gene            complement(1439..1990)
                     /locus_tag="VM910_21535"
     CDS             complement(1439..1990)
                     /locus_tag="VM910_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65141.1"
                     /translation="MTRRKIILGAVIGSAVLGVAALVGVAGHYAILKVDDDDDDDGRD
                     AVARGSRFAKISLQQGLIASEKEGQPISGKFEVDRAKFQLSIYTAKEGKFSEVLVDYS
                     TGQIAKVEPITKGEDLAAAQSQSAAMAKAKISLRDAVEKALGEAAGFRALGAVPNLKD
                     GRAVASVLLLKGEEFKIVNQALE"
     gene            complement(2427..2822)
                     /locus_tag="VM910_21540"
     CDS             complement(2427..2822)
                     /locus_tag="VM910_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65142.1"
                     /translation="MSTREKSTKRRLLVTALACLAVATLGANAAAEEKLQKLTGGQIR
                     AKLTGMELTDNVHWRDLYQRNGTVMSTSMGRKRTGKWRVEQNQLCIEFEKEPIPTCYD
                     VWISGKDVELRREGLLPLQGTLEPASGRN"
     gene            complement(2842..3186)
                     /locus_tag="VM910_21545"
     CDS             complement(2842..3186)
                     /locus_tag="VM910_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65143.1"
                     /translation="MLGVFAFQPPAAAEQKFQKLTGAQIQAKFPGMELTDEAHWGEVF
                     ERNGTLTITSMGHKSAGKWRVQKDQLCLDTGKEPGGDCYEVWLSGRNVELRNQASSTP
                     LEAVLQKPPPGR"
     gene            complement(3359..>4043)
                     /locus_tag="VM910_21550"
     CDS             complement(3359..>4043)
                     /locus_tag="VM910_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HVI65144.1"
                     /translation="GGWTAFKPAHGGAMVMGDLVLLETEINPVMAKLIENGIEITAVH
                     NHVLRANPLTFYMHIGGHGDPVKLATAIRTGLAASKTPLTPPAAPASQPAIDLDTAQL
                     DQIIGAKGQPNGGVYQFGVPRRDPVSESGTQLAPAGPTGVATAIGFQPTGGGKAAITG
                     DFVLTADEVNPVIQALRSNGIEVTAVHSHMLNEQPRLFFMHFWANDDAVKLAKGLRAA
                     LDKTASAKS"
BASE COUNT          711 a         1265 c         1112 g          955 t
ORIGIN      
        1 tggcaggact tcatgcagat gtcgtattca gtcttacatg tctttgcgga ataatttttc
       61 gcgcaggctt gttgttgatc tcgacactgc ttgcaagtgc tcgattcagc ttgtgctgca
      121 gtggtgatgc tgcaaacacc gaacaccaca acgataaccg cagcgatagg tcgcatgacg
      181 atctcctctc aatgttaggc aaccgctccc tgtgtaactc gctcaaagct cattgacttc
      241 tcttcctgcc ctcggctcca aactgcttca gataggccca gagatctccg atttccttct
      301 cgtcccttac tgaaagcaac atcgttgtgc ctgggacctt ggcagccgga ttgttgacga
      361 attccctaaa agcttgctcg ttccaagtga tgccggacat tctcattccc tggctatacg
      421 ttccgttctc gactgtgccc gccttcctgc catccagccc attgagttcc ggacccagct
      481 tgttcttcgc cgcctcgccg acgtcgtggc agggctgaca ttttcggaag gaggcttctc
      541 cagccgtcaa atcctgcgct aacgcggaac ctgccgatgc gactaggact gctgcgccga
      601 tcatcaaatg tctaaccatc atctgctctc ctcgttcacg aaaattcgag gcatgttccc
      661 ggcccgatcg tcggtcgaat gccctcgatt aaacggctgg cctgggcgat tttcgcccag
      721 gccggtgtcg gccccaagac aatccgacta cctcgcgttg ctcagctggc tgtcggctgg
      781 ccggagacaa ggtcgtagaa agtgggcttg atcgtactcc cctggactac catcacttcg
      841 aagacgggct tgccgctgcg tattttctcc ttaacggaca agaccgtgcc ctgcgccgag
      901 gccttctgga taatcgtcgg aagggtcgtc ttggtcatcg acagcagcgc atgatactgc
      961 gccgaccgag cgatgtgctt caggtcagcg aatacctcct tcttcggggc ccacgtcgtc
     1021 gtcgttgcgt cgccgatgta ctcgttgaag gaattatcct cggcggccgt gtcgaagccc
     1081 ttcgccgaag tgtagatcga cagcatcagc ttgccgtctt ccatttcgta tttcgcttcg
     1141 atgggcactt ccgtgccctt ggcgacttgg gcaatacctt gcagcaagct gtgcttggcc
     1201 ccgctcaagg ctttgatcag ggctgcctgg tctgcgtctt ctgaccaggc tgcactgctg
     1261 cttcccaaga ccgccagtcc gaaagcgata acgaacatca gagctctttt catcatgttg
     1321 aacctcctgt gtactgcgca gcatttgcgc ttctcgcaac tgccctctct tggccatcgg
     1381 ttatccttgc tggagcagcg gaaactcggt cggccgtcga cggctatcag cagaccacct
     1441 actccaatgc ttgattgacg attttgaatt cttcgccctt caggagcagc acggaggcga
     1501 cggcgcggcc atccttcaag ttgggcaccg cacccagcgc gcggaagccg gcggcttcac
     1561 caagcgcctt ctcgaccgcg tctctcaaag aaatcttcgc cttggccatc gccgcgctct
     1621 gcgattgggc agcggctagg tcctcgccct tcgtgatcgg ttcgaccttt gcgatctgac
     1681 cggtcgaata gtcgaccagc acttcggaga acttcccctc cttcgccgta tagatcgaga
     1741 gctgaaactt ggcacgatcg acctcgaatt tgcccgagat cggctggcct tccttttcgc
     1801 tggcaataag gccttgctgt aagctgatct tggcgaagcg gctcccccgg gcaacggcat
     1861 ctcggccgtc gtcatcatca tcatcgtcga ccttcaggat ggcgtagtgg cccgcaaccc
     1921 ctactagggc tgcaacgcca agaactgccg agccaatgac cgcccccagg atgatttttc
     1981 tgcgagtcat gctgacctcc gtcaattact tgcccgggat tgcggcggtt gcggtggatc
     2041 taaatggagt gcccgcagca aatgtcgccg tcagataaat gacaacgctg acagtcgttc
     2101 cgcccatcgg tcgctcaccc ggctatacgt ggacgaaagg cagcctcggt atcgacagcc
     2161 gtcacatccc gctttcgacc tgacgggcgt ttacttgtcg tttgtccgca aatgcgaatg
     2221 ttgcaccact cgaaagccgt ccggctactt acgctgtgag catttgttta aggcagtttt
     2281 aaacaaggca tctcgacctt cgtcggccgg gtggagacgc attgggatta ataggcctgc
     2341 aaaggagatt gggatgacca ttgccatgac acctgcgccg gatgttgttg ttcacgcaac
     2401 atacaaacgt accactcggt tcgggactag ttgcgtccgc tcgccggctc gagggtgccc
     2461 tgcaacggca acaaaccttc gcgtcgtagc tccacatctt ttccggagat ccatacgtcg
     2521 taacatgtcg gtatcggctc tttctcaaac tcgatgcaga gctggttctg ttcgactcgc
     2581 catttgccgg ttcgcttgcg acccattgaa gtgctcatca cagttccatt gcgctgatag
     2641 aggtcgcgcc agtgcacgtt atccgtcagc tccatgccgg tgagcttggc acggatttgc
     2701 ccacccgtaa gtttttggag tttctcttcg gcggccgcgt ttgctcccaa cgttgccacg
     2761 gcaagacatg caagcgctgt gacgagcaac cgcctcttcg ttgatttctc ccttgtgctc
     2821 atcgaagcaa tcctcggccg atcatcggcc tgggggaggc ttttgaagca cggcctccaa
     2881 gggcgtactc gaggcctggt ttctcagctc cacgtttctt ccggagagcc atacctcgta
     2941 gcagtcgcca cccggttctt ttccggtgtc gagacaaagt tggtccttct ggacccgcca
     3001 cttgccggca ctcttgtgac ccatggacgt gatcgtgagc gtgccgtttc gctcgaacac
     3061 ctcgccccaa tgcgcttcat ccgtcagctc catccccggg aatttcgctt gaatttgcgc
     3121 acccgtaagc ttctggaatt tctgctcagc cgcggcaggt ggttggaatg caaacacgcc
     3181 gagcaacgcg gtcgtcgctg ctgcaagttg tttttttcgt cgtgctgctc attgagccca
     3241 tcgcgcttct gatgacgacc gacgcaatcg gtgctcatcg atcgtctgcc gccttttcag
     3301 ggggccgagc gccgggccgc gcagttgcgg cccggccgtc aagtcgcttg ggcgacgctc
     3361 agctctttgc actcgccgtc ttgtccagtg cggcgcgcag tcccttggca agctttacgg
     3421 cgtcgtcatt ggcccagaag tgcatgaaga acagccgcgg ctgctcgttg agcatgtggc
     3481 tatgaaccgc ggtcacctcg atgccgttcg accgcagcgc ctgtatcact ggattgacct
     3541 cgtctgcggt caagacgaaa tcgccggtaa tcgcggcctt gcccccgccg gtcggctgga
     3601 agccgatcgc cgtggccacg cccgtgggcc cggcgggagc aagctgcgtt ccgctctcgc
     3661 tcacggggtc acgccgcggc acgccgaact gatagacgcc gccattgggc tgtcctttgg
     3721 cgccgatgat ctgatcgagc tgggcggtgt cgagatcaat cgcgggctgc gaggctgggg
     3781 ccgccggcgg cgtcagcgga gtcttgctcg ccgccagccc cgtgcggatc gccgttgcca
     3841 gcttcaccgg atcgccgtgt ccgccgatgt gcatgtagaa cgtcaggggg ttggcgcgca
     3901 gcacgtggtt gtggaccgcg gtgatctcga tgccgttctc gatcagcttg gccatcaccg
     3961 gattgatctc ggtttcgagc agcacgaggt cgcccatgac catcgccccg ccgtgggccg
     4021 gcttgaacgc ggtccagccg cca
//
LOCUS       DATLJT010000941         4085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1211591,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000941 DATLJT010000000
VERSION     DATLJT010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211591"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VM910_21555"
     CDS             <1..381
                     /locus_tag="VM910_21555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI65145.1"
                     /translation="ISFFPVVINTVVGLESVEENAMLLMRSLVASRRQIFWKLAWPAA
                     LPSIFAGLKTSLTLALIGALVAEFVTAKDGLGTLIITFSFELKVYLVFAVIVVVSVLG
                     LALYGIMEYLDRKVVFWRPSHFTG"
     gene            457..1737
                     /locus_tag="VM910_21560"
     CDS             457..1737
                     /locus_tag="VM910_21560"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65146.1"
                     /translation="MHRDFGLWHVGEDEAKVKAKGRTMRRRLSIMSATFALLAIALTA
                     YKASAPAASKEAPKPAQALTTLGVMRAAPTVPAVTEAPEKSAIRMAQARSVGGPPPLK
                     KFRVLLPFKVGITFFPISVANELGYLKDEGIDLDLQVANGSSAVVQQLAAGNAEIGVI
                     LAPNTLLGFSEGVKYKAFYDFLTKNTFDVKVIDSSPISRPSDLKGKSIGTIDLTRGDL
                     PLLRAELQRAGLNAQRDVQIVALGFNMSLHAQALKDGRVDALNISWNNTVSVEAVGVK
                     LKCITCDEQFQLASETTVAPDRIFQQDRRYIIGFGRAMAKATVFAETNPAAAIAIMKK
                     VAPQEHTDTAFTKTFFAAALAVMKPRQAGKYGLHDISGWERLQDFMAAPVEGQPTGLQ
                     NKVDVNQLVTNELVEEFNRFDVEPVRKQAMEYRP"
     gene            1970..2608
                     /locus_tag="VM910_21565"
     CDS             1970..2608
                     /locus_tag="VM910_21565"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65147.1"
                     /translation="MLQELRLVKHLHCAPGLYEEPLTAAQIFRMATENGARSIGFGDE
                     IGSIEPGKRADLVLLDYNRIAAPYLDPAISPIDAIIYRARNTDVDTVMIDGKIVFQDG
                     RTTRIDRSETLERIARSLDRRWSLRSSRDGASFRRSFRIFVISTSIGRSRSPNRSMRC
                     MERDRALRGPMRPEIVYVPIRDPEVRSAVLACCISDEGTPVDGRFGPLAGLA"
     gene            2822..3895
                     /locus_tag="VM910_21570"
     CDS             2822..3895
                     /locus_tag="VM910_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVI65148.1"
                     /translation="MTIEPWRATCIQTVSKLAVKAPHRDGAWAIIDENIAHALALIEA
                     ATQSADPPKLVVLPEFAFQGPPHGLPVANWIEKACCAIPGRISAPLQDLAHRAHIYVA
                     AHQFESVSQWPGRYFNTSFLIDPTGAVILRYRRVNTAAFPSPHDFMDAYLARHSMAEI
                     FPVVDTELGRLAMVPCGEINVPEVARVLMMQGAEVILHPTNSPRRPTQEAAKIVRAAE
                     NMVYIVSANVAEGIGFSWDGSVQGGRSHIIDYLGRTLAYEDGAGETTSVSALIDVEAL
                     REARKQDTGTANPLLRARWEMYRPFFAAASFYPPNSFLHTPMSDAASAAAALATSLRN
                     MASAGVIVDEPEVPIKKRAGEAR"
     gene            3892..>4085
                     /locus_tag="VM910_21575"
     CDS             3892..>4085
                     /locus_tag="VM910_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HVI65149.1"
                     /translation="MISEAENELLTRVGRKTPMGKFMRQFWIPACLSSELEADGPQMR
                     LMLLGEKLIAFRDTAGRVGI"
BASE COUNT          801 a         1259 c         1255 g          770 t
ORIGIN      
        1 atctcgttct tccccgtcgt gatcaacacg gtcgtcgggc tggagtcagt ggaggagaac
       61 gccatgctcc tcatgcgctc actggtggcg tcgcggcgcc agatcttctg gaagctggcc
      121 tggcctgcgg ccctgcccag catcttcgct ggtctgaaga cctcgctgac gttggcgttg
      181 attggcgcgc tggtcgccga gttcgtgacc gccaaggatg gcttaggcac gctcatcatt
      241 accttcagtt tcgaactcaa ggtctatctc gtcttcgccg taatcgtcgt ggtgtccgtg
      301 ctggggctgg cgctgtacgg catcatggag tacctcgacc ggaaagtggt gttctggcgt
      361 ccgagccact tcactggtta acaggaggaa ggctaatcgt ttcgcttcgg ttctgggttg
      421 cccgagcgtg agggattaag cctttgggaa gcaaccatgc atcgagactt cggactatgg
      481 cacgtcgggg aagacgaagc caaagtcaaa gcgaaaggac gaaccatgcg tcgtagactt
      541 tcgattatgt ccgcgacctt cgcgctcctc gccatcgccc tcacggccta caaggcgtcc
      601 gcgccggccg catccaaaga ggcaccgaag ccggcccaag ccctcaccac gctcggggtc
      661 atgcgagccg caccgacggt gccagccgta acagaggcac cggaaaaatc cgcgatcagg
      721 atggcccagg cgcggtcggt agggggcccg ccaccactta agaaattcag agtcctgctg
      781 ccgttcaaag ttggtatcac gttcttccca atcagcgtgg cgaacgagct cgggtactta
      841 aaagacgaag gcattgatct cgatctccag gtggcgaacg gcagcagcgc cgtagtccag
      901 cagctcgccg ctggcaacgc cgagatcggc gtgatcctcg cgcccaacac tctgctgggc
      961 ttctcggagg gcgtgaagta caaggctttc tacgactttt tgacgaagaa cacgttcgac
     1021 gtcaaagtca tcgacagcag ccctatcagc aggccatccg acctgaaggg caagagcatc
     1081 ggcaccattg acctgactcg aggcgacctg ccgctgctcc gcgcggagct ccaacgggcc
     1141 ggactcaacg cgcagcggga cgtccaaatc gtggccctcg gctttaacat gtcactgcat
     1201 gcccaagccc tcaaggacgg ccgggtagat gcgctcaata tctcctggaa caacaccgtc
     1261 agcgtggagg cggtcggggt taagctaaag tgcataacgt gcgacgagca atttcaactc
     1321 gcgtccgaga ctaccgttgc acccgaccgc atctttcagc aggatcgccg ctatatcatc
     1381 ggctttggtc gggccatggc gaaggcgacg gtctttgccg agacgaaccc cgccgccgct
     1441 atcgccatca tgaagaaggt ggcaccgcag gagcacaccg acaccgcgtt caccaagaca
     1501 tttttcgcgg ccgcgctcgc cgtcatgaag ccacgccaag caggaaagta cggcctgcac
     1561 gatatcagcg gctgggagcg gctgcaggat tttatggctg cgccggtgga aggccagccc
     1621 acgggcctgc agaacaaggt ggacgtcaac cagttggtga ccaatgagct cgtggaggag
     1681 ttcaacaggt tcgatgtcga gccggtccgt aagcaggcca tggagtacag gccatagtcg
     1741 cctacagcac gatctattcg tccttgcaat cgggcaatca gaccgttcag ctgttgcgca
     1801 gtccttcgcc tcgcccgaga ccatcaagcc ggagcggctg agcagtgacc gagcgtagcg
     1861 gtctggccgg gaccggcggc cgcaagcggc tgagtggcgc gaatgcgtcg ctggttcgcg
     1921 ggcggtatgt cttgtgcaag gcgcgcaacg accgggagct ggccgcgaca tgctgcagga
     1981 gctgcgcctc gtcaagcacc tgcattgcgc gcccggcctc tacgaggagc cgctgaccgc
     2041 cgcgcaaatc tttcggatgg cgacggagaa tggagccagg tcgatcggat tcggcgacga
     2101 gatcggttcg atcgagcccg gcaaacgcgc ggatctcgtg ctgctcgatt acaaccgcat
     2161 cgctgcgccg tatttagatc ccgccatttc gccgattgac gccatcatct accgagcccg
     2221 caacaccgac gtcgatacgg tcatgatcga tggcaagatc gtcttccaag acggccggac
     2281 gacccgcatc gatcggagcg aaacattgga gcggattgcg cgcagcctcg accgccgctg
     2341 gagcctgagg agctcgcgcg acggcgcttc gttcaggcga tctttccgca tattcgtgat
     2401 ttctacgtcg attggacgct cccgaagccc gaaccgttct atgcggtgca tggaacgcga
     2461 tagggcttta cggggtccga tgcgacccga aatcgtctac gtgccgatcc gggatcccga
     2521 agtgaggtcg gccgtgctgg cgtgttgtat ctcggatgag ggcacccccg tcgatggccg
     2581 tttcggtccg cttgcaggac ttgcgtagcg gcctcgagcg atggtaccgg cgtgcccagc
     2641 gctcgcgctc cggcccaggc cgacagcgga ccagtccttc caagccccgt ggcgcgctct
     2701 aagttttgct acggtcaata cgctttattg aaattgcatt gatcgcgcaa tgctatagcg
     2761 ttagccggag cgatcccgag cggcgtggcc gacttggcgg ttaaccattg aggcgccgga
     2821 tatgaccatt gagccatggc gggcgacttg catccagacc gtcagcaagc tcgcggttaa
     2881 ggctccccat cgcgatggtg cgtgggcgat cattgatgag aacattgcac acgcgctcgc
     2941 gctgatcgaa gcggcgacgc aaagcgccga tcctccaaag ctcgttgtgc tgcccgaatt
     3001 cgcatttcag gggcctccac atggtctgcc ggttgcaaat tggatcgaga aggcatgctg
     3061 tgcaattcct ggtcgcatca gcgcgccgct gcaggactta gcgcatcgag cacacatcta
     3121 tgtcgcagcc catcaattcg agagcgtttc gcagtggccg ggccgttatt tcaacaccag
     3181 ctttctgatc gatccgaccg gtgcagtcat tctgcgctac cggcgcgtca acaccgctgc
     3241 ctttccctcc ccgcacgact tcatggatgc gtatttggcg cgccacagca tggccgagat
     3301 ctttcccgtc gtagacacgg agctcggacg gctggcgatg gttccgtgcg gcgagatcaa
     3361 cgtgcctgag gtcgcgcgcg tgctgatgat gcaaggggcg gaggtcattc tccatccgac
     3421 gaattcaccg agacggccga cccaggaagc cgccaagatc gtgcgcgcgg ccgagaacat
     3481 ggtctacatt gtcagcgcga atgtcgccga aggcatcgga ttttcgtggg atggctcggt
     3541 gcagggcggt cgttcccaca tcatcgacta tctcggccgg acattggcct acgaggacgg
     3601 cgcgggagaa accacgtcgg tctcggcgct gatcgatgtc gaagcgctac gcgaagcgcg
     3661 caagcaggat acgggaacgg ccaaccccct gctgcgcgcc cgctgggaaa tgtaccggcc
     3721 gttcttcgca gcggcgtcgt tctatccgcc caacagcttc ctccacacgc cgatgtcgga
     3781 cgcagcctcc gcggcggcgg cgctggccac gtccttgcgc aacatggcaa gcgccggcgt
     3841 catcgtcgac gaaccggagg tgcccatcaa aaaaagagca ggagaagcca gatgatcagc
     3901 gaagccgaaa acgaactcct gacccgcgtc ggccgcaaga cgccaatggg caagttcatg
     3961 cggcagttct ggattccggc ctgtctttcg tccgagctcg aggccgacgg cccgcagatg
     4021 cgtttgatgt tgctcggcga gaagctgatc gcgttccgcg acacggccgg ccgcgtcggc
     4081 atcat
//
LOCUS       DATLJT010000942         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2130035,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000942 DATLJT010000000
VERSION     DATLJT010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130035"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VM910_21580"
     CDS             <1..336
                     /locus_tag="VM910_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65150.1"
                     /translation="LEFAVPLSLREGHGRSLAHFLRLAVAELERRDSILVEPIAAQSY
                     RDFLTNALLIYHPHNYGERLRRLERPITPRDVKRAIDYIQENLDTKIGLPEIVAASGI
                     PGRTDQAFS"
     gene            356..619
                     /locus_tag="VM910_21585"
     CDS             356..619
                     /locus_tag="VM910_21585"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI65151.1"
                     /translation="MRYLRSARFEKVHEALRRAEAKQNVTEIALQCGFTHMGRFSVEY
                     RKRFGESPSQTLRGYKTIIAVSDCVPAAAQDRAGGQGASQYPD"
     gene            complement(861..983)
                     /locus_tag="VM910_21590"
     CDS             complement(861..983)
                     /locus_tag="VM910_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65152.1"
                     /translation="MLAILPKLTIAGYREYAHFAAPEVLELYVAGLRLAGLPEK"
     gene            complement(1026..1238)
                     /locus_tag="VM910_21595"
                     /pseudo
     CDS             complement(1026..1238)
                     /locus_tag="VM910_21595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1361..1852
                     /locus_tag="VM910_21600"
     CDS             1361..1852
                     /locus_tag="VM910_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65153.1"
                     /translation="MPNRIGVIALGFERLSRQAAGFADVGGGQRSPPLSPLPHPTEPD
                     QSRGRGVGRVDRQRLPGEGDRIGKALFGEAMELRQCAQIEVISGEACGRLAAGARDFG
                     LAQFWFDRAGDIGRDLVLQIENVVERAIEAVAPDVRAGCRVDQLPGDATRWPALRTLP
                     SST"
     gene            complement(1792..2619)
                     /locus_tag="VM910_21605"
     CDS             complement(1792..2619)
                     /locus_tag="VM910_21605"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65154.1"
                     /translation="MTATRRLAAILAADVAGYSRLIGADESDTLTRLKAIRVELIDPT
                     IAAHNGRLVKTTGDGLLVEFGSVVDALRCASVVQAGMAERNAAAPADKRIEYRIGIHQ
                     GDIVVEDGYIFGDGVNVAARLEALAEPGGICVSARVQEDAAGRLDLAFENMGEQTLKN
                     IARPIRVYRVAMEPRADAANEPAPLPLPDKPSLAVLPFQNLTGDAEQEYFVDGMVEEI
                     TTAIARLSWLFVIARNSAFTYKGKPVDVKQVARELGVRYVLEGSVRKAGHRVASPGN"
     gene            complement(2616..>3171)
                     /locus_tag="VM910_21610"
     CDS             complement(2616..>3171)
                     /locus_tag="VM910_21610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI65155.1"
                     /translation="AASPHSDLVHGARGLVFRAQRRYAEAIPEYEAMLAANRNAVFAL
                     VSLGQCKTFTGTLEETIPLVEQAIRLSPRDPLIGHFYVEIGRVHLLRSRTGEAIAWLE
                     KSRGAMPEHSAFHAYLAAAYGLIGEIDRAAAELAVARRLALDDRFSSLSRLQAIGYWG
                     VPKIRALFEATYFAGLRLAGMPEE"
BASE COUNT          589 a         1002 c         1032 g          548 t
ORIGIN      
        1 ctcgaattcg cggtgccctt gagtttgcgg gaggggcacg gacgaagcct cgcgcacttt
       61 ctgcgccttg ccgtcgccga attggagcgg cgcgactcga ttttggtgga gccaatagcc
      121 gcgcagtcat accgcgattt cctgaccaac gcgctactga tctatcatcc ccacaactac
      181 ggcgaaagat tgcggcggct cgaacggccg atcacaccgc gcgacgtcaa gcgcgcgatc
      241 gattacatcc aggagaatct cgataccaaa atcggattgc ctgaaatcgt agcggcgagc
      301 gggatacctg ggcgcacgga tcaagcattt tcatgatttc aggggaactt cgcctatgcg
      361 ttatctgcgc agtgcgcgct tcgaaaaggt ccacgaagca ctgcgacgtg ctgaagcgaa
      421 gcaaaacgtc actgagatcg cgttgcaatg cggtttcacc catatgggcc gattctcggt
      481 ggaatatcgc aagcggttcg gcgaatctcc gtcgcagaca ttacgcggct acaaaaccat
      541 aatcgcggtc agcgattgcg tgccggcagc agcgcaagac cgtgctggcg ggcagggcgc
      601 gtcgcaatat cccgattgaa atcgacgagg taatcgaata accggtttcc acgcgacccc
      661 ggcgatggac ggcggctatg gggcacgaag agcggttccc gcccccaagg ctgaacggcc
      721 gttgtcggtt cggtcaagcg accttcaccg ggacgcacgg gaatgagcga gacgcgccgt
      781 ttccggacct ttccaccctc accccggaaa accggcaagt tcgacgcgta acgaccttca
      841 ggatcgtgga gtacggctac tcacttctcc ggcaggccgg ccagtcgcag gccggcgacg
      901 tagagttcga gaacttccgg ggccgcaaag tgagcgtact cgcgataccc ggcgatggtc
      961 agcttgggga ggattgcaag cactcgcgta agctcggcgc gggcctcatc gagccggccg
     1021 agatgcgcca acgcggcgac tttgacccgc atcgcggtaa ccatgcgcgg ctggtcgtgc
     1081 aaggcgcggt cggcccattc gatcgcctgt tcgaagcgtc gcgcggcgag atgagcgata
     1141 gcgatattac tcgcgtagaa aaggtatacg gatcgaatgg gctcaaacgg cgcgcccgtt
     1201 cgatggcttc gatcgccgcc tcgggctggt tgcgccatgc gtggatactc cctctggccc
     1261 accaggcgtg agccgagttg gggttgagcg ccagggcgcg atctagagcc gccgccgcca
     1321 tcgcggcctc gccggcgagg cgagacagcg tccaagcggc ttgccaaatc gtatcggcgt
     1381 catcgcgctc ggcttcgagc gcctgtcgcg ccaggcggca ggcttcgccg acgtcggcgg
     1441 cggacagcgc tccccacccc tgagcccgct gccgcacccg acagaacccg accaaagccg
     1501 cggccggggc gtaggacggg tcgatcgcca gcgcctgccg ggcgagggcg accgcatcgg
     1561 caaggccctc ttcggtgaag cgatggaatt gcgccaatgc gcgcagatag aggtcataag
     1621 cggtgaggct tgcggtcggc ttgcggctgg cgcgcgcgat ttcggattgg cgcaattttg
     1681 gttcgatcgc gccggcgaca ttggacgcga cctggtcctg cagatcgaaa atgtcgtcga
     1741 gcgcgccatc gaagcggtcg ccccagatgt gcgcgccggt tgtcgtgtcg atcaattgcc
     1801 cggtgatgcc acccggtggc cggctttgcg cacgctgcct tcgagcacgt agcgcacgcc
     1861 gagctcccgc gcgacctgtt tcacgtcgac cggcttgccc ttgtaggtga aggcggagtt
     1921 gcgggcgatg acgaagagcc acgacagccg ggcgatcgcc gtcgtgatct cctcgaccat
     1981 gccgtcgacg aaatactcct gctcggcatc gccggtcagg ttctgaaagg gcaagaccgc
     2041 gagcgacggt ttgtcgggca acggcagcgg cgccggttcg tttgcggcgt cggcgcgcgg
     2101 ctccattgct acccggtaca ctcgtattgg ccgggcgatg ttcttaagcg tctgttcgcc
     2161 catgttctcg aaggcgaggt ccagcctccc agccgcgtct tcctgcacgc gagccgagac
     2221 gcagatccca ccgggctcgg ccagggcttc cagacgagca gcgacgttca cgccatcgcc
     2281 gaagatgtaa ccgtcctcga ccacgatgtc gccctggtgg atgcctatgc ggtactcgat
     2341 gcgcttgtcc gcaggcgccg ctgcattgcg ctcggccatg ccagcctgaa ccacggacgc
     2401 gcagcgtagc gcatccacca cactcccaaa ctcgacgagc aagccatcgc ctgtcgtctt
     2461 gacgagccgg ccattgtgcg cggcgatcgt cggatcgatc agctcgaccc ggatggcctt
     2521 gagccgggtg agagtgtcgc tctcgtccgc cccgatcagt cgtgagtagc ccgcgacatc
     2581 agccgccagg attgcggcga ggcgacgggt tgcagtcatt cctccggcat tccggccagc
     2641 cgcagcccgg cgaaataggt ggcttcgaac aaggcgcgga tcttcggaac gccccaatag
     2701 ccgatggcct gcaggcgaga gaggctcgaa aatcgatcgt ccaaggccag cctccgggct
     2761 acggcgagct cggcggcggc gcgatcgatt tcgccgatga gcccataggc tgcagcgagg
     2821 taggcatgga acgcagaatg ctccggcata gcgccacgcg acttttcgag ccatgcgatc
     2881 gcctcgcccg tgcgcgagcg tagcagatgc acccggccga tctcgacata aaaatggccg
     2941 atgaggggat cacgcgggct gagccggatg gcttgctcga cgagcgggat cgtttcctct
     3001 agggtcccgg tgaacgtctt gcactggccg agagaaacca aagcgaacac agcgttgcgg
     3061 ttggcggcga gcatcgcctc gtactcggga atggcttcgg cataccgacg ctgcgcgcga
     3121 aagacgaggc ccctggctcc atgcacgaga tcgctgtggg gcgatgcggc c
//
LOCUS       DATLJT010000943         2811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_749910,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000943 DATLJT010000000
VERSION     DATLJT010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749910"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VM910_21615"
     CDS             complement(<1..502)
                     /locus_tag="VM910_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65156.1"
                     /translation="MPNLIPLHAWLIEAGTNDVATETLFDGFCGRLTAAGVPIARGFL
                     SIAGLHPLRRAYSLTWSDGRIVEVTDFGHARMATTVWQASPFRHMLETKTRRLHRRLV
                     GDDAVLDFEVLREFRDAGMSEWLALMYGFGWSAAQPATNQFGVVLSWTTSTAVGRGNG
                     WSALELA"
     gene            complement(770..1750)
                     /locus_tag="VM910_21620"
                     /pseudo
     CDS             complement(770..1750)
                     /locus_tag="VM910_21620"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
     gene            complement(1906..2628)
                     /gene="phoU"
                     /locus_tag="VM910_21625"
     CDS             complement(1906..2628)
                     /gene="phoU"
                     /locus_tag="VM910_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HVI65157.1"
                     /translation="MVHSRHTTKVFDSDLQELARAIAEMGGLAERQIAEAIEALTTRD
                     NGPAQRIVAADATIDAMQRNIEKRAVETIARRQPVAGDLREIVGILRIANEIERIGDL
                     AKNIGKRIIAINGEDASRGSMRGVSQMAALMLAQLRDVLDSFACRDVAKAVEVWTRDQ
                     DIDRLCTSLSQELLAYMMEKSVAMSFGAHLMFCAKNLERMGDHATNIAEAVHYMVKGE
                     TLLRERPKADFTSMLTVAMGRT"
BASE COUNT          540 a          887 c          841 g          543 t
ORIGIN      
        1 ccgccagttc cagggcactc cagccattac cgcgtccgac ggcagtggaa gtggtccacg
       61 ataaaacgac gccgaactga tttgttgctg gctgcgcggc gctccagccg aacccgtaca
      121 tgagagctag ccattcgctc atccctgcat cgcggaactc gcgcagtacc tcgaaatcga
      181 gcacggcatc gtcgcccacc agccgtcggt gcaatcgccg cgtcttggtt tcaagcatgt
      241 ggcggaacgg acttgcctgc cataccgtcg tggccatgcg cgcatggccg aaatcggtga
      301 cttcgacgat tcgcccgtca ctccacgtaa gcgaatacgc ccgccgcagc ggatgcagcc
      361 ccgcgatgga cagaaacccc cgcgcgatcg gcacgcccgc cgcggtcaac cgcccgcaga
      421 acccgtcgaa cagcgtttcc gtcgctacgt cgttagtccc ggcctcgatg agccaagcat
      481 gcagggggat gaggttgggc attaataaca aatctggcat gcgctagaag gatggcgtgt
      541 aggcccgcat tgcgcaacgc tttcgcattg acgatcccgc caacggttct cgcgctcgcc
      601 gagtaaatca ttgcaacccg acccgccagc tgggggcgcc aaccgcgcgg gatctgcatg
      661 cgcagacttt ttgctgtcag cggattccgg ccaatgatcg aatgcgaatg ggcatcgtcc
      721 caggtgcggg ggttgcgaac ctggggccag gcggcccgct gcgccatatc taaggcgata
      781 cgatgattct tgtctgtatc tgagccggtt gtttcacccg ccgccaagtg tcatccgggt
      841 caaagctcgt catttccaag gcgatatcgg gggtgcgcca gccgaggtcc ttctcgtaga
      901 tggtcccctg atgattgacg aggaacgtca tcaggccgga actgcggtat cgcgcgggaa
      961 aggccacgag cgcgaacccg ccgaccatgt cgccgctcgc catatagtcg acttcgccgc
     1021 ccggggcgtt cggcccttgc cgggtgagaa tcttatagta atagccctga tagggagccc
     1081 accgcttgcc ttcgacggaa tatcctttct ctgcagcagc ggtgccgaat tcgccgagcg
     1141 gactttcgcc ttcaccggac ggtgctggcc agtagaggcc gtctctttct cctggtcggc
     1201 tgataaaacg ccgtgcgtag acgcccttac ctgcgaaacc cttctcggca tattccttct
     1261 gggcggcgac gtaggcttca caggcgccga tcgcgctctc ctcgttgcgg ccgatgcggc
     1321 ggtaaacaat ttccgagcgg gccagctgcg catcgaatcg ccaagtattg ttcaggcgca
     1381 ccagccggat ggggaacggc cagcgttctt ccccgacaat cagcgcggcc tagtcctcgc
     1441 cgtccggaac gatctcatac ttggcctcga agtcggcgag gaaacgcctg cgcatggccg
     1501 catcgtcaac agggtcgccg gacagcacga tctcgtcgct gcctggaccc agcacgcgca
     1561 tcaattgctg cagggcatcg gtccgcactg cgtcggcgag cgccgcgacc gcttcgtcgg
     1621 gagtgcggta gcgcccctgc gctagggcgc cggtcgcaac agtgaggaac accgtcgtca
     1681 ctgcaagggc tgcgagcggc aggacaaagg gaaaccggtg gggtcggaga actgagggga
     1741 gcaagatcat gacattgctc catctgcggc gctcgatctg cggcgcctga gagatgatac
     1801 cgccgattca cggtcgcgca aaggtcgccc ggcggcgctt gataagaaat ggccgaatta
     1861 cgtcgcggag gttgttggag gatggacaca ggagccggag cgtggtcacg tccgccccat
     1921 cgccacggtc agcatgctgg tgaaatccgc ttttggtcgc tcgcgcaaga gcgtctcgcc
     1981 cttcaccata tagtgaacgg cttcggcgat attggtcgcg tgatcgccca tgcgctcaag
     2041 attctttgca caaaacatca ggtgcgcgcc gaaggacatc gcgaccgact tttccatcat
     2101 ataagcgaga agctcctgcg ataaactcgt gcacagccgg tcaatatctt ggtcccgcgt
     2161 ccaaacttcg accgccttcg cgacatcgcg gcacgcgaag ctgtcgagga catcccgcaa
     2221 ctgagccagc atgagagcag ccatctggct gaccccgcgc atcgagcccc gcgacgcgtc
     2281 ttcaccgttg atggcaatga tccgcttgcc gatattcttg gcaagatcgc caatgcgctc
     2341 gatctcattc gcaatccgca agatgccaac aatctcgcga aggtcaccgg ctaccggctg
     2401 ccggcgtgcg atggtctcaa ccgccctttt ctcaatgttg cgttgcatgg catcgatggt
     2461 cgcgtcggcg gcgacgatcc gctgcgcagg accgttgtcg cgcgtcgtca gcgcctcgat
     2521 cgcttcggca atctgccgct cggcaagccc acccatctcg gcgattgccc gcgcgagttc
     2581 ctgcaggtca gagtcgaaga ctttggtggt gtgtctggaa tgaaccattg tggtgggacc
     2641 aatgcaagcc gcagggggca catgacgcgc aaggtccttt tataatggtt cgatgtccgc
     2701 gcgatgacga tgaagagccg cacacaatcg cgttggcgtt tcaagcgacc agcggcgacg
     2761 catgatcttc tcattcactt cgtgcgccgt gcggatcgat tgcagtacta c
//
LOCUS       DATLJT010000944         4581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3739279,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000944 DATLJT010000000
VERSION     DATLJT010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3739279"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..765
                     /locus_tag="VM910_21630"
     CDS             193..765
                     /locus_tag="VM910_21630"
                     /inference="COORDINATES: protein motif:HMM:NF042552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6647 family protein"
                     /protein_id="HVI65158.1"
                     /translation="MFKLLPCCLALLAIAIGESADIQLRASGTQSIEQQHFRPKTLLT
                     PLETWVSSQFDLPAIHEHPSIEFASPAKIVSLRFAGLLSDPGEQVAPNDRASSAQHDT
                     IAIYYDATRTIYLPEGWTGDTPAELSVLVHEVVHHFQNVLRLKYECPQEREKLAYIAQ
                     DRWLALFGHSLESDFHMDAFSLLVKTQCFW"
     gene            complement(883..1788)
                     /locus_tag="VM910_21635"
     CDS             complement(883..1788)
                     /locus_tag="VM910_21635"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65159.1"
                     /translation="MPVIGVLHGTSAAQWADRMAGFHRGLGEAGFAEGRNVTIEYRWA
                     EGQFERLPAMAADLVSRKVAVISAGAPDVALRAAMAATKTIPVVFVTASNPVGAGFVP
                     SLGRPEGNVTGITLIGAELVPKRLELLRELLPGATRIAVLVNPNNPGLMRDNIQQSEA
                     AVRRLGLEMVVIEAGTESEIESAVVAAVQQQASALSIATDAYLNSHSRQIASFALRHA
                     LPTTGESRETVAARLLMSYGPNQVDSYRQAGLYVGRILKGEKPADLPVLQPTKFELVI
                     NLTTAKAVGLKISESFLLRADEVIE"
     gene            complement(1943..3175)
                     /locus_tag="VM910_21640"
     CDS             complement(1943..3175)
                     /locus_tag="VM910_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65160.1"
                     /translation="MSGRKTVTIMSALTRRQAMAGGTATLIGAPFVIRSAFAQRPVVN
                     IGVIQPLSGANAQFGINSRNGIELVADGINAAGGVKALGGAKINLVVSDATSNPTTAS
                     TAAQRLIAQNELTAILGAYASSLTLAISEVTARADIPFLTQAYADEITGRGLESVFKV
                     TPAASVFGRAQINYTLAIAQAAGGKIEKIAIMYEDTAYGVAQSRGLRRAAKDANIEVV
                     MDEAYPLGITDATPLIDKLRASGAQAVFPLSYLNDSLFIVRTMRRERITVPVIGGSAG
                     YVIPDFEKGFGELAEDVFSISPTNYDLAPAFTDPFRKRFGYFMVQEAIGHAVALDVLV
                     QAIVRAKSAKPKLVTEALHGVRFEGGWTEVMPGGAVQFDQAGLNTLSIPIMVQWRNKE
                     LVTVWPKGIAKASPVWHS"
     gene            complement(3291..4286)
                     /locus_tag="VM910_21645"
     CDS             complement(3291..4286)
                     /locus_tag="VM910_21645"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65161.1"
                     /translation="MKRREFITLLGGAAAALPRTVVAQDTDRARRIGVLMELAANDPQ
                     ARSNVAALKRGLQKLGWSPGGNLGIEYRWAPDDAVLVWKFAKELVESQPDVIVAHSSP
                     VVATLLGQTRDIPIVFVSISDPIGEGFVASFSRPGGNVTGFTNFESSVTGKWVELLKD
                     FAPELTRVAFLFNPQTAAGGGSYFLRPIDAAASTLKVKAAMALVHDDDEIEAAFAALA
                     REPGVGAVLLPDIFTVAHHQLVIALAARYRVPTVYGYRFMVERGGLISYGVDIDNLFE
                     RAAAYVDRILKGAKPSDLPVQAPTKFELVINQKTARALGLPVPDALLARADEVIE"
BASE COUNT          929 a         1403 c         1323 g          926 t
ORIGIN      
        1 ctcggcattt ggttcgtccg ccttgtaagt aggctatcaa cactcagtca cgcgggctgg
       61 ttcaagctcg gagccccagc acgtccctct gcagagcgcg agaagtccac gcaaaccggc
      121 taaccgcaca gaccgtcatg aagagaagcg atgtaggtgg tcgcattttg ccgtcatctt
      181 taggagaacg cgatgtttaa actactcccg tgctgcctag cactgctggc catcgcaatc
      241 ggcgaatccg ctgatatcca actccgggca agcggcactc aatccattga gcagcaacac
      301 ttcaggccga aaaccttgct taccccactc gaaacttggg tttcgagtca attcgacctt
      361 ccggctatcc acgagcatcc cagtatcgaa ttcgcatctc ccgccaagat cgtgtctttg
      421 cgctttgccg gcttgctgtc cgatccagga gaacaagtgg caccgaacga tcgagcctcg
      481 tctgctcaac acgacacgat cgccatctac tacgacgcca cgcgaactat ctatctgcct
      541 gagggctgga ccggcgacac accggccgag ctatccgtgc ttgttcatga ggtggttcat
      601 cactttcaaa atgtgctgag gctcaaatac gagtgcccac aggagcgcga gaagcttgcc
      661 tatatcgctc aggaccgctg gctcgctcta tttggacata gtcttgagag cgactttcat
      721 atggatgcgt tttcgttatt ggtgaaaaca caatgttttt ggtagataag cgcgttcgac
      781 ggcttcatca gccgaaatcg aggattaatt cctgccgcgc gcgagccgcg agcggccacg
      841 ccgctgccgc gccgccgagc agcgtgatga acgcgcgacg cttcattcga ttacctcgtc
      901 ggcgcgcagc aggaaggatt ccgagatttt cagcccgact gccttggcgg tcgtgagatt
      961 gatgaccagc tcgaacttag tcggttgcaa gaccggcagg tcagccggtt tttcgccctt
     1021 gaggatgcga cctacataga ggccggcctg ccgataactg tccacctgat tgggaccgta
     1081 gctcatcaat aagcgggcgg cgacggtttc gcgagactca cccgtcgtgg gcagcgcatg
     1141 gcggagtgcg aaagaggcta tctggcggct gtgcgaattc aggtaggcat cggtagcgat
     1201 gctcagagcg ctagcctgct gctgcacggc agcgacaacg gcgctttcga tctcgctttc
     1261 agtcccggcc tcgataacga ccatctccag cccgaggcgg cgcaccgcgg cctctgactg
     1321 ctggatgttg tcccgcatga ggcccggatt attcgggttc acaagaacgg caatccgggt
     1381 ggcgccggga aggagctcac gcagcaactc cagccgcttc ggaacaagct ccgctccgat
     1441 aagagtgatg cccgtgacgt ttccctccgg gcgaccgagg ctgggcacga aacccgcgcc
     1501 gaccgggttg gaggccgtca cgaacacgac agggatcgtc ttcgttgccg ccatcgccgc
     1561 ccggagagcg acgtctggcg cacccgcact gataaccgca accttgcggc tgacgaggtc
     1621 ggcggccata gcaggcagcc gttcgaactg gccttccgcc cagcggtatt caatggtcac
     1681 gttccgaccc tcagcgaagc ccgcctcgcc taggccccgg tggaatccgg ccatgcggtc
     1741 tgcccattgc gcggccgacg tgccgtgcag cacgccgatc accggcattg tcgcctgctg
     1801 cgcgcgcgcc gcaagcggcc acgcggccac cgcgctgccg agaacggtga tgaattcccg
     1861 ccgtctcaat tcaagcatcg tggcttccct ctcgccgtga cgtaggtcta ttcgcagaat
     1921 ggccactgaa cgatccacct attcaggagt gccacaccgg ggacgctttc gcgatgcctt
     1981 tcggccagac tgtcacgagc tccttgttgc gccactggac cataatcggt atcgaaagcg
     2041 tgttgaggcc ggcctgatcg aactgaactg cgccgccggg cattacttcg gtccagccgc
     2101 cttcgaaccg cacaccgtgc agagcttcgg tcactaattt cggctttgcc gacttcgctc
     2161 tcacgatcgc ctgcacaagc acgtcgagag ccacagcgtg cccaatcgct tcctgcacca
     2221 tgaagtagcc gaagcgcttg cggaacgggt ccgtgaacgc gggggcgaga tcgtagtttg
     2281 tgggcgagat cgaaaacacg tcctctgcca actcgccgaa ccctttctcg aaatcgggaa
     2341 tgacgtaccc ggccgatccg ccgatcaccg gtacggtgat gcgctcgcgt cgcatagtgc
     2401 ggacgatgaa gaggctatcg ttgagatagg agagtgggaa taccgcttgc gcgccagagg
     2461 cgcgtaactt gtcaatgagc ggtgttgcgt cggtgatgcc gagcggatat gcctcatcca
     2521 tcaccacctc aatgttggcg tcctttgcgg ccctgcgcag acctctggat tgtgcgacac
     2581 cgtaagcggt gtcctcgtac ataattgcga tcttctcgat cttgccgccg gccgcttgcg
     2641 caatcgcgag cgtgtaattg atctgcgcgc gaccgaatac cgaggctgcg ggggtcacct
     2701 tgaagacgct ctccaagccg cgcccggtga tctcgtcagc gtacgcctgg gtgagaaagg
     2761 gaatgtctgc ccgcgccgtc acctcggaaa tcgcgagcgt caacgacgat gcgtatgcac
     2821 cgagaatcgc agtgagctcg ttctgggcta tcagccgttg ggcagcagtc gacgctgtgg
     2881 tcgggttcga agttgcgtcg ctgacgacta agttgatctt ggcgccgccg agcgccttga
     2941 cacccccggc cgcgttgatg ccgtcggcga cgagttcgat accgttgcgg gaatttatgc
     3001 cgaactgagc attggctccg gataacggct ggatcacccc gatattgact accgggcgct
     3061 gcgcaaaggc agagcggatg acgaacggcg ccccaatcaa tgtggcagtc ccaccggcca
     3121 ttgcttgtcg gcgagtcaaa gcgctcatga ttgtcactgt cttgcgtccc gacatgtcct
     3181 cctctcattc aattattttg ctcatatcga tctcacgcaa cacacgttct cttgccctgc
     3241 cgggccggac ttccaccagc tgatcgcgcc agtttgcctg gcgccgttac tcattcgatc
     3301 acctcgtcgg cgcgggcgag caatgcgtcc ggcactggga gacccagcgc cctagcggtt
     3361 ttctggttga tgaccaactc gaacttggtt ggtgcttgca ccggcaagtc agaaggcttg
     3421 gcgcctttga ggatgcgatc gacatacgcg gccgcacgct cgaacagatt gtcgatatcg
     3481 actccatagg agatcagacc accgcgctcc accatgaaac gatagccata gaccgttggc
     3541 actcggtaac gcgccgctag agcgatgact agttggtgat gagccacggt aaaaatgtct
     3601 gggagcagga ccgccccgac accgggctcg cgtgcaagag cagcaaaagc cgcttcgatc
     3661 tcgtcgtcat cgtgaaccag cgccatggcg gcttttactt taagggttga agcagccgca
     3721 tcgattggac gcaagaaata tgatccgcca ccagcggccg tctgggggtt gaagaggaag
     3781 gcaacgcgcg tgagctcggg ggcaaagtcc ttgagcaact ccacccactt tccggtcacc
     3841 gaggattcga aattggtgaa cccggtgacg ttaccaccgg gacgcgaaaa gctcgcgacg
     3901 aagccctcgc caatggggtc ggatattgac acgaatacaa ttggaatgtc ccgggtctgc
     3961 cctagcaggg ttgccacgac gggactcgaa tgggccacga taacgtcggg ttgtgactcg
     4021 acaagttctt tggcgaactt ccacacgagt accgcatcgt cgggcgccca gcggtactca
     4081 atcccaaggt taccgcccgg cgaccatccg agcttctgaa gtccccgttt caacgccgcg
     4141 acattggatc gtgcctgagg atcattagcc gcgagctcca ttaatacacc gatccgccga
     4201 gcccggtcgg tgtcttgagc tactacggtg cgcggcaatg cggcagcagc gccgccgaga
     4261 agcgtgataa actcgcggcg tttcattcag tcaccaaggc gttgcccatg ccggtttcca
     4321 tcgctcgtcc gccttggttg agagaccgcg gaagccaata aacgccccat tttccatgaa
     4381 gcgctgaact ccacctaacg tatttgatct ggctcaattc tgagcatgta gttttttgga
     4441 cgcacgtagc tctcaaggtg cagtctcggc ctgaagcact gcgcatctgc gaggacgctc
     4501 atgacacgga aaccgtggcg actaatggct tatggcagcc gcgaccagca gagcagtgca
     4561 ccgatcggta aggcggcatc a
//
LOCUS       DATLJT010000945         5732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_293636,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000945 DATLJT010000000
VERSION     DATLJT010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293636"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VM910_21650"
     CDS             complement(<1..1331)
                     /locus_tag="VM910_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI65162.1"
                     /translation="MQTPREILADLWRLAGGEPSALEAVKLTGEEPQLPSSFRVAAAA
                     QVSIAAAGLAAARIWKLRSGEAQSVAVDMTHAVAECRSERYLRVDGKPPPPAWDPTAG
                     IYRTRDRRFVRLHTNFPHHRAAVCKVLQCKEEREAIQSALMQWDGEAFETAAYAGGAV
                     VSLMRSHEEWSATQQAVALAKLPPFTIEKIGAAPPKPWPKGDRPLAAVRVLDLSRVIA
                     GPVAGRTLAVHGADVMLISGPKLPAIPWLTIDTGRGKLSSFLELKDEADRARLRALLQ
                     DADIFSQGYRPQAIASLGFSPEDVARLNPGIVYVSLSAYGHAGPWAERRGFDSLVQTS
                     TGFNHAEGQAAGIDRPKELPAQMLDHATGYLMAFGAMMAKARQSREGGSYLVRVSLAQ
                     TGRWLWGLGRIADGLKTEDLKGEAIRPFFEDMPSGFGPLSAISHSARLSATP"
     gene            complement(1448..5056)
                     /locus_tag="VM910_21655"
     CDS             complement(1448..5056)
                     /locus_tag="VM910_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVI65163.1"
                     /translation="MTGPVDYAEIGITTNFSFLRGGSHPQDYVHQASELRMPAIGIAD
                     HNTLAGIVRAYSELDNPEVRHKPKLLIGARLVFADGTPDILAYPRDRAAYGRLCQLLT
                     RGKRDAEKGECNLKLADLLEFAEGQLLVLALPHRFEADGALKLLTRLRESRAEGVWLA
                     ASLLYRGDDKRRLERLGHLAQTAHVPLLATNEVLYHHPSRRPLQDVLTCIREKTTIEA
                     IGKRLEANAERYLKPADEMARLFCDHAHAIAETMRFANRIRFSLDQLKYQYPDEPVPP
                     GKTAQQHLEDLTWAGARKYFPDGIDEILRATLRKELDLIAELKYAHYFLTVHDIVHYA
                     RSQGILCQGRGSAANSAVCYVLGVTSVDPTKVDLLFERFISKERLEPPDIDVDFEHSR
                     REEVMQYVYRRYGRHRAAVIATVIHYRPRSAIRDVGKALGLSEDVTSLLADTVWGSWG
                     DGLSEGQVRQAGFDPGNAMVELAVALASELIEFPRHLSQHVGGYVLTQDRLDTYVPIG
                     NAAMEDRTFIEWDKDDVDALHMMKVDVLALGMLTCIRKSFDLIENHKGERWALATIPQ
                     DDAAVYDMLCRGESLGVFQVESRAQMNMLPRLKPRVFYDLVIEVAIVRPGPIQGDMVH
                     PYLRRRNGIEKVSYPSPSPEHGEADELYRVLHKTLGVPLFQEQAMRIAIEAAKFTPEE
                     ANGLRRSMATFRNLGTIGKYESKLIGNMIARGYAPEFAKSCFDQIKGFGSYGFPESHA
                     ASFAQLVYVSSWLKHYHPDAFCCALLNSQPMGFYAPAQIVGDARKNGVEVREVDVSCS
                     FAENTLEETDGRYCAVRLGFRQIDGFHWLDEDEERLKQVQRSFRDAPASPLSFRGATK
                     SRAMMRNCASENPYSLATAVLQLPPPERGRGGEGVRRTYQYNGDIDPLPARSGAQERV
                     ADLADLPLSGGGEEAVVVDDWAARIVAARKRRPFTSLEDFARDTGLPKRALILLADAD
                     AFRSLGLDRRAALWAVRRLPDDVPLPLFEQAAAREQPDEHARPLPSMPRSEHVVADYQ
                     TIRLSLKGHPMEFLRAMFAKERVLPCREVCHRNDRLTVRCAGVVLVRQRPGSAKGVVF
                     MTLEDETGIANIVVWPKVMEKFRKEVMGSRLILVEGYIQSSPEEVTHLVAQRLIDRSH
                     DLLALSDDRLRKHAVPAGPALIEPLNDDRRDHPENPAQKIRHPRNVRVLPPSRDFH"
     gene            complement(5053..>5732)
                     /locus_tag="VM910_21660"
     CDS             complement(5053..>5732)
                     /locus_tag="VM910_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HVI65164.1"
                     /translation="VDTGQPVTRAAVVGRLFRERLEALNDPLDPGFGFDLIRLSASHT
                     EIVVEQQHDLDTHVHDNDELVALIDRIAARIGSRRVFVHLPLETHIPERAVLTAPAQH
                     HLASAAAASWPERATGEPPSRPLRLFDRPEPIKVPLATVPDGPPHHFTWRRAVHAVVK
                     VEGPERIAMEWWKQDGKMLTRDYFRVEDETGLRFWIFRDGLYGSELLDEEGRPAPANW
                     FVHGLFA"
BASE COUNT         1025 a         1843 c         1858 g         1006 t
ORIGIN      
        1 ggcgtcgccg aaagccttgc cgaatggctg atggcactga gggggccgaa gcccgagggc
       61 atgtcttcga agaaggggcg gatagcctcg cccttcaaat cctcggtctt gagcccatcg
      121 gcaatgcgac cgaggcccca aagccagcgc ccggtctgcg ccagcgagac gcgcaccagg
      181 taactgccgc cctcgcgcga ttgccgcgcc tttgccatca tggcaccgaa cgccatgaga
      241 tagccggtgg cgtggtcgag catctgcgcc ggcaattcct tcggcctgtc gatgccggcc
      301 gcttgccctt cggcatgatt gaatccggtc gaggtctgca ccagcgaatc gaagccgcgg
      361 cgctccgccc aggggccggc atggccgtag gccgacagcg acacatagac gatgcccgga
      421 ttgagcctgg ccacgtcctc cggcgagaag ccgagcgagg cgatcgcctg cggtcggtag
      481 ccctgcgaaa agatgtcggc atcctgcagc agcgcgcgca gccgcgccct gtccgcttcg
      541 tccttcagtt cgaggaagct tgaaagcttg ccacggccgg tatcgatggt gagccagggg
      601 atcgccggca gttttggacc tgagatcagc atgacgtcgg cgccgtgcac cgcgagcgtg
      661 cggccggcga cggggcctgc gatcacccgc gacaggtcga gcacgcgcac ggccgcaagc
      721 gggcgatcgc ctttgggcca tggttttggt ggggcggcgc cgatcttctc gatcgtgaag
      781 ggcggcagtt tggccagcgc cacggcctgc tgcgtcgccg accattcctc atgcgagcgc
      841 atcagcgaaa caacggcgcc gccggcatag gccgcggtct cgaaggcctc gccatcccac
      901 tgcatcagtg cggactgaat cgcctcgcgc tcttccttgc attgcaagac cttgcaaacg
      961 gcggcgcggt ggtgcgggaa attggtgtgc agccgcacga agcgccgatc gcgcgtgcga
     1021 tagatgccgg cggtcggatc ccacgccggc ggcggcggct tgccgtcgac gcgcaaatag
     1081 cgctcggagc ggcattcggc aaccgcatgg gtcatatcga ccgcgacgct ttgcgcttcg
     1141 ccgctgcgca gtttccagat tcgagccgcg gcgagccccg ccgctgcgat ggatacttgc
     1201 gccgcggccg cgacgcgaaa cgacgacggc agttgcggct cctcgccggt gagcttgacg
     1261 gcctcaagcg cagacggctc gccgcccgca agtcgccaga ggtcggccag gatttcgcgg
     1321 ggtgtctgca tggaggaatc cgccggttgc gagcgcccct tctacgccgg ctacgcgaag
     1381 caatccagcg acaggagatc aggtctgcat tgcttcggag ttcgcactcc tcgcaacgac
     1441 gggaccatca atggaagtcc cttgacggcg gcaggacgcg cacattgcgc gggtggcgga
     1501 ttttttgcgc cgggttttcg ggatggtcgc gccggtcgtc gttgagcggc tcgatcagcg
     1561 caggccccgc aggcaccgca tgcttgcgga gccggtcgtc ggacaaggcc agcagatcgt
     1621 gcgaacggtc aatcagccgc tgcgcgacca gatgcgtcac ttcctcgggg ctactctgga
     1681 tatagccctc caccaatatg agacgtgagc ccatcacttc tttgcggaat ttttccatca
     1741 ccttcggcca caccacgata ttggcgatgc cggtttcatc ctccagcgtc atgaacacca
     1801 cgcccttggc gctgcccgga cgctgccgca ccaggaccac gccggcgcag cgcacggtga
     1861 gccggtcatt gcggtgacag acctcgcggc agggcagaac gcgctctttt gcgaacatcg
     1921 cacgcaaaaa ttccatcggg tggcccttca gcgagaggcg gatggtctgg tagtcggcga
     1981 ccacatgttc ggatcgcggc atcgacggca gcggacgcgc atgctcgtcc ggctgctcgc
     2041 gggcggcggc ctgctcgaac agcggcagcg gcacgtcgtc gggcagccgc cgtaccgccc
     2101 agagtgcggc gcggcggtcg agcccgagcg atcggaacgc atcggcatct gcaagcagga
     2161 tcagcgcgcg cttgggcaag cccgtgtcgc gggcgaaatc ctcgagcgag gtgaacggac
     2221 ggcgcttgcg cgccgcgacg atgcgcgcgg cccagtcgtc tactaccacc gcctcttcac
     2281 ctcctcctga aagggggagg tcggcgaggt ccgcgacgcg ctcttgcgcg ccggagcgag
     2341 ccgggagggg gtcaatatct ccattatatt ggtacgttcg tcggaccccc tccccacccc
     2401 tccccctttc agggggaggg agttgaagca ccgccgttgc cagggaatat ggattctcag
     2461 atgcgcaatt gcgcatcata gctcgcgact tcgtcgcgcc ccggaatgac agtggagagg
     2521 caggcgcatc ccggaacgac cgctgcacct gcttcaaccg ctcctcatcc tcatcaagcc
     2581 agtgaaagcc gtcgatctga cggaagccga gccgcaccgc gcaatagcgc ccatccgtct
     2641 cttccagcgt gttttcggcg aacgagcagg agacgtcgac ctcgcgcacc tcgacgccgt
     2701 tcttgcgggc gtcgccgacg atctgcgcag gcgcataaaa acccatcggc tgcgaattga
     2761 gcagcgcgca gcagaatgca tcgggatgat aatgcttcag ccatgacgag acatagacga
     2821 gttgcgcaaa gcttgcggcg tggctttcag gaaagccgta ggagccgaac cccttgatct
     2881 ggtcaaagca gcttttggca aattccggcg catagccgcg cgcgatcatg ttgccgatca
     2941 gcttggattc gtatttgccg atggtgccga gattgcggaa agtcgccatc gagcggcgca
     3001 ggccgttggc ctcttcgggc gtgaattttg cggcttcgat cgcaatccgc atagcctgct
     3061 cctggaacag cggcacgccg agcgtcttgt gcagcacccg gtagagttcg tccgcttcac
     3121 catgctcggg cgatggcgag ggatagctca ccttctcgat tccgttgcgc cggcgcagat
     3181 agggatgcac catgtcgccc tggatcgggc cgggccgcac gatcgccact tcgatgacga
     3241 ggtcgtaaaa cacccgcggt ttcaggcgcg gcagcatgtt catctgcgcg cggctctcga
     3301 cctggaacac gccaagcgat tccccccggc acagcatgtc gtagaccgcc gcatcgtcct
     3361 gcgggatggt cgcaagcgcc cagcgctcgc ccttgtggtt ttcgatcagg tcaaagcttt
     3421 tgcggatgca ggtcagcatg cccagcgcca gcacgtcgac cttcatcatg tgcagagcgt
     3481 cgacatcgtc cttgtcccat tcgatgaagg tacggtcctc catcgcggca ttgccgatcg
     3541 gcacataggt gtcgagccgg tcctgcgtga gcacataacc gccgacatgc tgtgacagat
     3601 ggcggggaaa ctcgatcagc tcgcttgcga gcgcgaccgc aagctcgacc atcgcattgc
     3661 cgggatcaaa ccccgcctgc ctgacctgcc cttcggagag gccgtcgccc caactgcccc
     3721 agacggtgtc ggccaaaagc gaggtgacgt cttcggacaa gccaagcgcc ttgccgacat
     3781 cgcggatcgc gctgcgcgga cgataatgga tgacggtcgc aatgaccgcg gcgcggtggc
     3841 gtccgtagcg gcggtagaca tattgcatca cctcctcgcg ccgcgaatgc tcgaaatcga
     3901 cgtcgatatc gggcggctcc agccgctcct tggagatgaa gcgctcgaac aggaggtcga
     3961 ccttggtcgg atcgaccgag gtgacgccga gcacgtaaca gaccgcggaa tttgccgccg
     4021 atcccctgcc ctggcacaaa ataccctggc tgcgcgcata gtgcacgatg tcgtgcacgg
     4081 tcaggaaata atgcgcgtat ttcagctcgg cgatgagatc gagctccttg cgcagggtgg
     4141 cgcgcaatat ctcatcgatg ccatcgggga aatatttcct ggcgccggcc caggtcaggt
     4201 cctccagatg ctgctgggcg gtttttcccg gcggcaccgg ctcgtccgga tactggtatt
     4261 tcaattgatc gagcgagaag cggatgcggt ttgcaaaacg catggtttcc gcgatcgcgt
     4321 gcgcgtgatc gcaaaacagc cgcgccattt cgtctgcggg cttgagatag cgttccgcat
     4381 tggcctccag ccgcttgccg atcgcctcga tcgtggtctt ctcgcgaatg caggtcagca
     4441 cgtcctgcag cggacggcgc gagggatgat gatacagaac ctcgttggtc gcgagcagcg
     4501 gtacgtgagc cgtctgcgca aggtggccga gccgctcgag gcgccgcttg tcatcgccgc
     4561 gataaagcag gctcgccgcc agccacacgc cctcggcgcg gctttctcgc aagcgcgtga
     4621 gaagtttcaa cgcgccatcg gcttcgaaac ggtgcggcag cgcgagcacc aaaagctggc
     4681 cttcggcaaa ctccaggagg tcggcgagtt tcaagttgca ctcgcccttc tcggcgtcgc
     4741 gcttgccgcg ggtcagaagc tggcacagcc ggccgtaagc cgcgcgatcg cgcggatagg
     4801 ccaaaatatc cggtgtgccg tcggcgaaga cgaggcgcgc gccgatcaga agctttggct
     4861 tgtgccgaac ctcgggatta tcgagttcgc tataggcgcg cacgatgccg gcaagcgtat
     4921 tgtgatcggc gatgccgatg gccggcatgc gcagttcgct cgcctgatgc acatagtcct
     4981 gcggatgcga cccgccgcgc aggaaggaga aattggtggt gatgccgatc tcggcgtaat
     5041 caacaggacc ggtcatgcga acagcccgtg cacgaaccag ttggcgggcg ccggcctgcc
     5101 ctcctcgtcg aggagttcgc tgccgtaaag accgtcgcga aagatccaga agcgcaggcc
     5161 cgtttcatcc tcgacgcgga aatagtcgcg cgtcagcatc ttgccgtcct gtttccacca
     5221 ttccatggcg atgcgctcgg gcccttccac cttcacgacc gcatgaaccg cgcggcgcca
     5281 ggtgaaatga tgcggcggcc cgtcgggcac ggttgcgagc ggcaccttga tcggctccgg
     5341 ccggtcgaac agccgcaacg gccgtgacgg cggttcgccc gttgcgcgct ccggccaact
     5401 cgccgccgcg gcagaagcaa gatgatgctg cgcgggagcg gtcagcaccg cgcgttccgg
     5461 gatgtgggtc tccagcggca aatgcacgaa gacacggcgg cttccgatcc gcgccgcgat
     5521 gcggtcgatc agcgcgacca gttcgtcgtt gtcgtgaaca tgggtgtcga gatcgtgctg
     5581 ctgctcgacg acgatttcgg tatggcttgc cgacagccgg atcaggtcga agccgaagcc
     5641 gggatcgagc ggatcattca aggcttccag gcgctcgcgg aacagccggc cgacgacggc
     5701 agcgcgcgtg accggctggc cggtgtcgac gg
//
LOCUS       DATLJT010000946         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_523443,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000946 DATLJT010000000
VERSION     DATLJT010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523443"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..189
                     /locus_tag="VM910_21665"
     CDS             37..189
                     /locus_tag="VM910_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65165.1"
                     /translation="MKKKVARRIAVMSAMIRRMSGLEARPVMRDAEMAKIDGPDAQAE
                     DELIRD"
     gene            291..431
                     /locus_tag="VM910_21670"
     CDS             291..431
                     /locus_tag="VM910_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65166.1"
                     /translation="MQNISRAALKHLEGKGKIIRLHIDKAMLMKLGASFIVGKAKLAG
                     HL"
     gene            complement(540..1004)
                     /locus_tag="VM910_21675"
                     /pseudo
     CDS             complement(540..1004)
                     /locus_tag="VM910_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2809..>3180
                     /locus_tag="VM910_21680"
     CDS             2809..>3180
                     /locus_tag="VM910_21680"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65167.1"
                     /translation="MTVASDVPSFKLITVDDYEPLVGAETIERILQKASRLRDLHVAH
                     VNSTYYGGGVAELLSSLTLLMNSAGIKTGWRVIQGRPDFFSITKKMHNALQGGNINLT
                     DLKLQIYEEVAFENAVRNHLDH"
BASE COUNT          671 a          919 c          970 g          620 t
ORIGIN      
        1 cgatgaggcg cacgccaaag ccggcctgct tgcggcatga agaagaaggt ggctcgacgc
       61 attgccgtca tgtcggcgat gatccgccgt atgtcgggtc tcgaggcgag gccggtgatg
      121 agagacgcag aaatggcaaa aatagacggc ccagatgcgc aagcggagga cgagttgata
      181 cgtgattgaa aatgagccct gaagccagct agccattcga cccgagctga ggaagcgccc
      241 gacggaacgc aaaggcatgg ggcagtgcgt tccctgggaa cagcgctcga atgcaaaaca
      301 tatcgcgcgc cgccctaaag catctggaag gcaagggcaa aataatacgc ttgcatatcg
      361 acaaagctat gctgatgaag cttggtgctt cgtttatcgt ggggaaggcg aaacttgccg
      421 gtcacctgtg atgggatttt ggcggaatga tccgccgaaa tttcactgta aaatcttcca
      481 tgctgtcgct gacgccaagc tttcgagcgg ttgcgacgaa ccgctcgaat tgctgcttgt
      541 cagtgctttt cggacgcttc ttttgctttt ggcctgcttt tcccatacgg ccactcagtg
      601 gtgaggcgtt ccatgggcga ggcgcttagg gcgcgcgcgg tgcgctcacc gtcgtctact
      661 cctagcttgg ctcggttgct acagcgagaa tcaaattcag cgagatagcg gtgcaaatgt
      721 ttctcgctgc aatgctggta cacgccatgc atcccgcgct tgaagatcga aaagaaattt
      781 tcggcggtgt tagtatgcac gtcgccgcga acgtattcct cttgtttgtg tttcacagcg
      841 tcatgtcgcg cgaactcgcg gccaacttcg tgataatagg tgccttcgtc gggcatcatg
      901 gttggttcgc gtgcaagatt ggcgcggatg accggcgcaa tgtcggcaat gcgagtgcct
      961 tccacatgga agctacgggc tgaaccgcca cgatggatta tactaaaact cggagagcct
     1021 gactcatgct ggcccgcgcc accaccgaga cagccattgc aaaaacgtcc agcggcccaa
     1081 tgggatatgg gagtcttgct ccgaacaccg agcatgcgcg catgagtcaa tgtctccgca
     1141 ggctagtatt aggctcagca cgacacgctt gtggacggga atccccgaaa tccccgaaat
     1201 gacaactggc gagctatgca gaggtcctgc atggctcggg ttgtcacacc tgtcccacgt
     1261 ctcggccgca cgtgtgatag cgagaatgca ggaactgcgc aaagaggcct atcccttgag
     1321 tcccttgggc ggcggaccaa aaactggcca ggaggtaggc gcagactttg gtatcatcca
     1381 gcgcgttgtc atcgatgaac cgggtaacgt ccctttccat ccgtgtgtag gctcccgcag
     1441 ccctagcacg tcgcaagcat cgataccgag tctcggcgcg agatcgcatc gcggcacatc
     1501 agcgatagcg atagtgaatt cgtgtctgga ctaggtccgc cagtatcacc gccaccggcg
     1561 ctgcggcgtc ggagccgcgc gacaccaagc tgcatcctac agcgcgcgag atcggcatcg
     1621 gtcttgaacc catagctgcg gcaccggcta tcgtcggtgg ctcgctgcgc aacctcacga
     1681 cgttgttctg cctgccgggt aagggcgcac ccagtcacgg ccagcccgat catgacggct
     1741 gcaacgatcc ttgatgttgc gcccatcgcc tccctcctgg tgcgatgatg atcagatcgc
     1801 gcggccctgc tattttagag catgggtgac ccataagctc ggggcagcga ccagcgcgac
     1861 ctcggccgcg atgtaggcga tgcgcgcgat gacggcaagg agccaagggc agagcgagct
     1921 tgcccaggac cgatctcgag cgcggtgttc gacgccgttg gcgtgcgact gcgttccgtg
     1981 cccttcacgc cggacaaggt caaggcggcg atgaaaggag cgggtgcagc gaggctagct
     2041 gccattcttg cgggtgacca tatcgcgagc gagttgcttg tccgcgaccg gtcccaggcc
     2101 cccgacagcg ccacgcgcct gacgcggtcg ggaatgcgag cggcgcaggc aaaggcgccc
     2161 caccagacac tccgacgata gcaaaccggc ccagtgcgat ctgatccgcc aattctgcca
     2221 catctgtagc ccaggcaccg atcgttcggc ctggctggaa tgtggattcg ccgaaaccgg
     2281 gacgatcggg ggcaatcagg cgcagaccga gccgtcgggc ggtgtccgcc gccgagcggc
     2341 cctcaagccg cgaacccgga taaaagactg ggctgcccgt ggggtcgcag gccagggtgc
     2401 gcccatcggc gagccggatc tggtacgtcg tctgccgaag agccatgacg cagggcgtca
     2461 caggccctgt gatcggcgtg cgggaatgct gatcaagcaa actccggctg cgaccatcga
     2521 gaccgcgcgc tcacattgtg cgcgctattt cggaacagcg tggcagctgt cgcgagctga
     2581 tgctgccgga atcggtgtcg tttggccgaa cctagctatc atgggcgcgc ttggtggtgc
     2641 ttttctcgcc gcagctgtat ctcgttttag ggcgatgctt gcgcgacaga cttgatcgaa
     2701 ggggtgccga caggccagac acgtgaccgc tgcgaaggat ggtctttaca cccaagaacg
     2761 ccagctaagg caaccattat tacggcggcg cacgccggga gactgagtat gacggtagcc
     2821 tcagacgtac cctcgttcaa gttgatcaca gtggatgatt atgagccact ggtcggcgcc
     2881 gagactattg agcgtatcct ccagaaggca tctcgccttc gcgacctgca cgtagcccat
     2941 gtcaactcga cctactacgg cggcggggtt gccgaactct tgtcttctct cacattgctg
     3001 atgaacagcg ccggaatcaa aaccggatgg cgggtgatcc aaggccgccc tgactttttt
     3061 agcatcacca agaagatgca caacgcgttg cagggtggaa atatcaatct gacggatttg
     3121 aagctgcaaa tatacgaaga agttgcattc gaaaacgcag ttcgcaatca cctcgaccac
//
LOCUS       DATLJT010000947        10425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3280185,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000947 DATLJT010000000
VERSION     DATLJT010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3280185"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..767
                     /locus_tag="VM910_21685"
     CDS             393..767
                     /locus_tag="VM910_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HVI65168.1"
                     /translation="MLVDEVPAPPSHLSSSAQQWWRTVVDRYRLEEHHLRLLQLACEA
                     WDRNQQARERVAEEGLTVFGREGGMRPHPAVAIERDSRLAVARLIRELDLDTEPPVSE
                     RIGPPGLLSNRGRRNAGKGANS"
     gene            complement(1099..1428)
                     /locus_tag="VM910_21690"
     CDS             complement(1099..1428)
                     /locus_tag="VM910_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65169.1"
                     /translation="MDREALVREHKLRELIAELDQAADRLIRCAGTLEPAKVRDELGR
                     VSVAIEPARTLLVGSQAFDLDELLGVEQWQYDEAAATYLTQVDARATANTIRKAAAYL
                     HELLRTR"
     gene            complement(2049..4448)
                     /locus_tag="VM910_21695"
     CDS             complement(2049..4448)
                     /locus_tag="VM910_21695"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HVI65170.1"
                     /translation="MTTLEPDRDQLEIFVEGTFRHCGSDGVVSLRAFFESDSSKPFRI
                     TNVPLKGGLPFLIEAVEDDARRAANDPKPVVFCPPVATFAPTGRAREIDLFEAPVLSV
                     ELDQHPRDALERLEHLLGFATLVVRSGGVWTDPATGEIEDKLHAHWRLKEPARGADIA
                     KLKRARQLATAIVGGDPTNVPACHPIRWPGSWHRKATPRLCEIINTEHLDNELDLDTA
                     LAALEAAAPQPKTNGQGGPQQSVSQQSGNDRLDWSEAFGKIITGKEFHPTLVPLAASL
                     AARGTPEVVTRGVLRALLANTQTNDSERLRRRDVELSKLKDTVHSGYEKFAATPTSSA
                     LFDPWQEFIAPPFPLDILPGAAHDFVATKSVAMGADPAALAMAQLAVLSGAIHHRCRV
                     KMMKNSDWWEHVRLWVLLFGRSSWLKSPIMEAMLRPIRRAEADLQRDYKARLQSWKAQ
                     DGNKDEEPEPPVRLLVGDTTTEKLCEIISRADRGMLSEHDELAGWIARMERYHTAGKG
                     ASTDRAFYLRCWNGGVYTYDRKNSGEILVPNASLSILGGIQPQRLDELQGLTSDGLLQ
                     RFAVVLMQAPKEPQDIDCTEVTKAYAALVYELLKLPGQRFYLTESAADAMAELQHHLH
                     SLEQVGAALTEAFEGHIGKLKAYAGVFTIILHLIANPSEAMRLSAIGRPAVEKAARLI
                     KEFLLPHAYEFYGRCEGEGEQLRTIASYVLASSLSRIRLADFTTNVRYCRGKKVLEIN
                     QQVSPLVAGDWLAPIGPGPACRAWTVNRAVIDQQFATRMASERESKRAITQLLGSRRQ
                     A"
     gene            complement(4445..5002)
                     /locus_tag="VM910_21700"
     CDS             complement(4445..5002)
                     /locus_tag="VM910_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65171.1"
                     /translation="MGRLIDLIGKRFDRWEVLAIHPERRRHGKAGKAIAVLWRCRCDC
                     GVEGIVYGSNLRQRLSKSCGCAAREATRRRNTTHGHAVRGQHTRAYEAWQHAKQRCYN
                     RNNQDYSNYGGRGIGIHPDYRDDFLAFFADVLDPPEGLSLDRIDNNRGYEPGNLHWAD
                     AATQVRNRRAEGVFDHFGARRGGTA"
     gene            complement(5265..5588)
                     /locus_tag="VM910_21705"
     CDS             complement(5265..5588)
                     /locus_tag="VM910_21705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65172.1"
                     /translation="MHGVTTTSRSTNASGPNLRKLLRGKPPRIRAVAAAKLVTGEWLF
                     TRPLPAQAARLCRVTPALVSKALGRPPRRRSDHELDRLVIRLGADRVLASLDRITRPV
                     IGGAS"
     gene            complement(5803..6003)
                     /locus_tag="VM910_21710"
     CDS             complement(5803..6003)
                     /locus_tag="VM910_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65173.1"
                     /translation="MARPRKAPPEELPDALALRRRIPVQEAAAILGIHEDTFRKHYSG
                     LIRKIGPRLERVELGEVFAIGK"
     gene            complement(6651..7229)
                     /locus_tag="VM910_21715"
     CDS             complement(6651..7229)
                     /locus_tag="VM910_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI65174.1"
                     /translation="MKIWGALDDGDYSSIIRLLVLTLCRRNEIGHLAFDEVDFDVGVI
                     RLPGERTKTGEARDVPMSAPVRRILQARHQQWDGRRTLVFGTGEGGFQGWARAKQNLD
                     ARAGVTGWTHHDLRRFGSTTMNNEGIATPFVIEAVLGHAVGNRVARTYNLATYSEQVR
                     AAMELWADRVMQLVSGERRPTKVLALRRRKGA"
     gene            complement(7126..7890)
                     /locus_tag="VM910_21720"
     CDS             complement(7126..7890)
                     /locus_tag="VM910_21720"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HVI65175.1"
                     /translation="MKLTEPMTLTRDAVTKLALPAGKPEHTVWDDKIAGFGIRLREHS
                     ATYFFRYRHGKRQPRITIGAVSAITNAKARAKAEELYARAKFAEDPAGTRTEARKRAN
                     ETFALALKPYLIRRQAEMRKRSFINLNRHLMKHAAPLHRLELAKAAERRRVAELLAKI
                     ATDSGPVEANAVKGSLNKFFSWAIGQGLLDGTGIDIASHGRHGQSTDQRTAYARSHRG
                     RSCEDMGRAGRWRLQQHHQIACVNAVPAQRDWASGL"
     gene            8272..8454
                     /locus_tag="VM910_21725"
     CDS             8272..8454
                     /locus_tag="VM910_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65176.1"
                     /translation="MFLQLVAGGHLLLFITRNERWFFLPPFPALPLFGAIVLTQILAV
                     LMCGLGAGSSRRSPGR"
     gene            8409..8606
                     /locus_tag="VM910_21730"
     CDS             8409..8606
                     /locus_tag="VM910_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65177.1"
                     /translation="MRLGGWLVPEIPWTLIGWVWLYNIAWMFVLGGVRLLTERFAAYR
                     TARQTRSMQIVNQSLRLHATP"
     gene            8621..9499
                     /locus_tag="VM910_21735"
     CDS             8621..9499
                     /locus_tag="VM910_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HVI65178.1"
                     /translation="MDYRKRFLVEPGAKVRLSKIEASYTGKNETHEKALPKIQKHAER
                     MDKLQYLLYADGSQSLLVVLQALDAAGKDGTIRHVFSGMNPQGTFVFGFKQPSKEELA
                     HDFLWRAHLRTPAKGEVVIFNRSHYEDVLVVRVHKLVPRSVWSKRYDLINDFETMLSQ
                     NGTTILKFFLHISPEEQLARFKQRLDDPSRRWKISESDYSERELWPQYVEAYEDAMAL
                     TSTKRAPWYIIPANHKWFRNLAISQIIADTMDDMGLKLPPTRVDIADIRRKYHAAARE
                     RKISDHRRAKKADKAA"
     gene            9763..>10425
                     /locus_tag="VM910_21740"
     CDS             9763..>10425
                     /locus_tag="VM910_21740"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVI65179.1"
                     /translation="MHPWPPPAPPPYAELVTLLALMVGAILVVVGLLRAGWLATLLSI
                     PVTTGFLAGIAIHIMIGELPTLLGISEERGHMLLRLFHILGRLDEANLYTLALGAGVL
                     IVTLGTARISAKVPGALIGVVAAGVAVALFHLQSRGVSLLEPLHVELPRLALPSLPEA
                     RDIGRLLPLALIVAMVCIMQTSAVASTFPSEEDRPDDASRDFAGVGAGSIVAGLTGSF
                     PVD"
BASE COUNT         1828 a         3249 c         3282 g         2066 t
ORIGIN      
        1 tcccatcctg catcactcca aggcgttacg taatgcccct atggggtccg gtgtaaggaa
       61 cctgtgctcc ccgggtggcc gagatttcta taaatcgccg ggcatcgaag agagtgagat
      121 gccaagcttt ctcatgatgt ctcttttggc acttctcgga cctcgcgaga tgtccgaccg
      181 cagaagcgcg gatgagcctt gatcgagagc gacagtccgc cgaccgtcgt acggggggtg
      241 gcgggttctc ccgcgctata gtcctccgtg cgccattgcc gtaccgaggg gggcgatgaa
      301 aaatttcgcg aggccggcaa cctccggccg cggagctggt ccggccttga tgcgccttgc
      361 ccccgctcaa aaactgagaa taggctaaca aaatgctcgt cgacgaagtg cccgcaccgc
      421 ctagtcacct ctcatcgtcc gcccagcagt ggtggcgaac cgtggtcgac cgctatcgat
      481 tggaggagca ccacctgcga ctcttgcagc ttgcgtgcga agcctgggat cggaaccagc
      541 aagcacgcga gcgggtcgca gaggaaggat tgacggtgtt cgggcgcgag ggcggcatgc
      601 gcccacatcc agccgtggcc attgaacggg attctcggtt ggccgttgcg aggttgatca
      661 gagaactcga cctcgacacc gagccgccag tctcggagcg catagggccg ccgggattgc
      721 tctccaacag gggccgccga aatgccggta aaggcgcgaa cagctaaaac gctgcggcca
      781 tcattcagtg ccgaggtgct cgaacttttc gtcgagcttg agcacgctcc acggcggcgt
      841 actgaggaat tccaagccaa agagaagcga ctcgcgagcc tgcttaattt ggttccgcat
      901 tggtgggcaa cctgctcggt gctcgataaa agcccttgtt gctggacgcc ctcctacgcg
      961 gcgtatggat actggcacga gtgtcgccta gtccgtgagc agctcctggc cgcgaccgga
     1021 agcgcgagcg tgccgagctc gcggcaaact gcggttcaat aaggcccccg gcatccgccg
     1081 tcccaccggg cggccgcctc accgcgtcct gagcaactcg tgcagatacg cggcggcttt
     1141 ccgaatagtg ttggcggtcg cgcgagcatc gacctgcgtg agatacgtcg ctgcagcctc
     1201 atcgtactgc cactgttcga cgccgagaag ctcatccaaa tcgaaggcct gtgatcccac
     1261 gagaagcgtg cgggctggct caatcgcgac cgagacgcgc ccgagctcat cgcgcacctt
     1321 cgccggttcg agcgtgccgg cgcacctgat gagacggtca gccgcttggt cgagctcggc
     1381 gatgagctcg cgcagcttat gctcgcgtac gagggcttcc ctatccatcg agcctcctcc
     1441 gccgtcgctg ccaccacggc ggctcctcct gccgcttctc ccggctagac cgcgaccggc
     1501 cagcctgcgc gcgccgcttg tggcgcggcg aaaccttggg ccgcgtcaaa ccgacgcgaa
     1561 cgcgaccctt gcttgccctt gctcattcga agcctcccgt cgctcgcgcc cggattgcga
     1621 cacgcgcttg gccctgcgac catcgaggcg catgctggcg tccgggtttg agcctttgtt
     1681 ccacgaccac gcgatagacc gtcacatcgt cggcgaggga gtctgtcgag gataaacatt
     1741 gcccctgcct tgcagctacg gcggggatgc tttcgaagct cgtcaacata ggcgtcttgc
     1801 acccaagcgg tgatcacgca atcgcttcgg cgccgtttcc tccccggctc ccaccttctt
     1861 cgtcatcacg accttttccc ttattgcata gctcgcgcga tgcgcttttt ccctgtctga
     1921 caaatctgac gcaaatcgac catacgtata gagacagaaa aagagagaga gttcctattc
     1981 tatgtttgta tacgcgcacg cggacagatt tgtcagatca acgatatatc ttaccgcctc
     2041 agagctggtt aggcctgccg ccgcgatcct aggagttgtg tgatcgcgcg cttgctttca
     2101 cgttcgctag ccatgcgcgt agcaaattgc tgatcgatga cggcgcggtt cacggtccag
     2161 gcgcggcatg ccggtccggg tccaatcggg gccaaccagt caccggcgac cagcggcgag
     2221 acctgttggt tgatctcaag caccttcttg ccccggcagt agcgtacgtt ggtcgtgaaa
     2281 tcggcgagac ggattcggct taggctactg gcgaggacgt agctcgctat cgtccgtagc
     2341 tgttctccct cgccttcgca gcggccgtag aactcataag catgcgggag caaaaattct
     2401 ttgatcaagc gcgcggcctt ctccaccgcc ggacgaccta tcgcgctgag ccgcattgcc
     2461 tcgctcgggt tggcgatcag atgcaggatg atggtgaaga cgccagcata ggccttgagc
     2521 ttgccaatgt ggccctcgaa agcctcggtg agcgccgcgc caacctgttc cagactgtgg
     2581 agatggtgct gcagttcggc cattgcgtcg gcggcagatt cggtgagata gaacctctgc
     2641 cccggcagct taagcagttc ataaacgagc gcggcatagg ccttcgtcac ctcggtgcag
     2701 tcgatgtcct gcggctcttt tggtgcttgc atgaggacga cagcaaaccg ttgcagcaat
     2761 ccatccgagg ttaagccttg tagctcgtcc aggcgctgag gttggatgcc accgaggatg
     2821 gaaagcgagg cgttgggcac gagtatttcg ccgctgttct tccggtcgta tgtgtacact
     2881 ccaccgttcc agcaccgcag gtagaacgca cggtctgtcg acgcgccttt tccggccgtg
     2941 tggtagcgct ccatccgcgc aatccatccg gctagctcat catgctcaga gagcatcccg
     3001 cgatcagctc ggcttatgat ctcgcagagt ttctcagtcg tggtgtctcc aacaaggagc
     3061 cgaactggcg gttctggttc ttcatctttg ttgccgtctt gcgctttcca gctttgcagg
     3121 cgggctttgt agtcgcgctg caggtcagcc tccgcgcgcc ggatcggccg caacatggcc
     3181 tccatgattg gggacttcag ccacgaagac cgtccgaaca ggagcaccca caggcggacg
     3241 tgttcccacc agtcgctgtt cttcatcatc ttgacgcggc agcgatggtg aattgcaccg
     3301 ctgaggacgg cgagttgcgc cattgccagc gccgctgggt ctgcgcccat ggcgacgctt
     3361 ttcgtggcga cgaaatcgtg cgccgcaccg ggcaagatat cgagcgggaa aggcggcgcg
     3421 atgaattcct gccacggatc aaaaagagca ctgcttgtcg gcgttgcggc aaatttctcg
     3481 taacccgaat gcaccgtgtc cttgagcttg ctcaactcga catcgcggcg gcgcaagcgt
     3541 tcggaatcgt tggtctgagt gttggcgagc agcgcacgca gcacgccccg cgttaccact
     3601 tcaggggtcc cgcgtgcggc aaggctcgcc gcgagcggca caagcgtcgg atgaaattcc
     3661 ttgcctgtga tgatcttgcc gaaggcctcg ctccaatcga gacggtcgtt gccgctttgc
     3721 tggctgacgg attgttgcgg gccgccttgg ccgttggtct tgggttgcgg tgcggcggct
     3781 tcgagggctg cgagcgcggt gtcgagatca agctcgttat cgagatgctc ggtattgatg
     3841 atctcgcaca agcgaggcgt ggctttgcga tgccaagacc ccggccatcg gatgggatgg
     3901 caggccggga cgttggtggg atcgccaccg acgattgcgg tcgcgagttg ccgcgcgcgt
     3961 ttgagcttgg cgatgtcggc accacgggcg ggttccttca gccgccaatg ggcatgcaac
     4021 ttgtcctcga tctcccccgt cgccggatcg gtccataccc cgcccgagcg gacaaccagc
     4081 gtcgcaaagc cgagcagatg ttcgagacgt tcgagggcgt cgcggggatg ctggtcgagt
     4141 tcgaccgaga gcaccggcgc ttcgaatagg tctatctcgc gagcgcgccc ggtcggcgcg
     4201 aacgtggcaa ccggcgggca gaacacgacg ggcttggggt cgttggcggc gcggcgggca
     4261 tcgtcctcaa ccgcctctat taggaatggt aaccctccct tgagcgggac attcgtgatg
     4321 cggaacggct tgctgctgtc gctctcgaag aacgcccgca agctgacgac gccgtcgctg
     4381 ccgcaatggc ggaacgtgcc ttcgacaaag atttcaagct ggtcgcggtc gggttcgagc
     4441 gtcgtcatgc tgtccctccc cggcgcgcgc cgaaatgatc gaagacgccc tcggcgcgac
     4501 ggttgcggac ttgcgtcgcg gcgtcagccc aatggagatt gcccggctca taaccgcgat
     4561 tgttgtcgat gcgatcaagc gacaggccct ccggcggatc gagcacgtcg gcgaagaaag
     4621 ctaaaaaatc gtcgcgatag tctggatgaa tgccgatgcc gcgaccgccg tagttggaat
     4681 agtcttggtt gttccggttg tagcaacgct gttttgcatg ctgccaagcc tcatacgcgc
     4741 gagtgtgttg gccccggacg gcatgaccgt gcgttgtgtt gcgtcttctc gtcgcctcac
     4801 gcgcagcgca gccacagctt tttgaaagtc gctgacgtag attgcttccg tacacgatgc
     4861 cttccacacc gcagtcgcat cgacagcgcc aaagaacggc aatcgccttg cccgccttgc
     4921 cgtggcgacg gcgctccgga tggatcgcaa gaacctccca gcggtcaaat cgcttcccga
     4981 tgagatcaat cagtcgaccc atgactcgct ccccgtgcgt cgcttcggct ctttcaaaaa
     5041 gaacggcggc accctcgcga gcgccgccga gaaacacggc taacggtctg cgttggtatc
     5101 ctggccgctg caggcagggc acgggcagga gttgtcttcg cgtaggtcgt cggcgagata
     5161 gtcggcggcg tcggcaatgg cgatgagcgt ctcgcctttc tgcctgagga ggctgcgcag
     5221 cctgctatcg cggacaagct ggtcagctag atgctggacg ggggtcacga cgcacccccg
     5281 atcaccggcc gcgtgatgcg gtcgagcgac gccaacacgc gatcagcgcc gagccggatc
     5341 acaaggcggt cgagctcgtg atcggaccga cgccggggcg ggcggccaag cgccttgcta
     5401 acgagggctg gggtcacgcg gcacaagcga gctgcctgcg cgggtagggg gcgggtgaag
     5461 agccactcgc cggtcacgag cttcgctgcc gcgactgccc tgatgcgcgg cggcttccca
     5521 cgcagcaact tgcgcaaatt ggggccgctg gcgttcgtag atctagatgt ggtagtcaca
     5581 ccgtgcatgg ccttctcctg gctgtgcgac caagtgttgg gaaaaccggg tccggttggt
     5641 cgcgaagacg accggacacg ataagtccgg cttgtcagag ccggcgtgag gttctccaga
     5701 tcagcggccc tgggttcgca ttccgggtcc gggccgcacc tgccgcgcaa cacgcaacat
     5761 cccttcgagc cgccgcgcga cgagcagccg aaaagcaaac cgctattttc cgattgcgaa
     5821 cacctcgcca agctcgacgc gctctagccg aggcccaatt tttcggatca ggccggaata
     5881 atgtttcctg aaagtgtcct cgtggatgcc gaggatcgcg gccgcctcct ggacaggaat
     5941 tctccgacgc aatgcgagcg catcgggcaa ctcctccggc ggtgccttac gcgggcgagc
     6001 catttgttca cctccaaaca tctttgttcg gaagttaccg acttcgcgtg cgcgcggcga
     6061 ggagaacttg cgtcgcgtag gggggataaa gagggtgaaa gcgtttcacc gtgacacggt
     6121 gtcagggtgt cagggtgtca gggtgtcacg gtgtcagggt gacacggtgt cacggttcac
     6181 gagtttccac ccttcatcgc atcaaccggt cgacagaacg tcgctagaaa ccgggcctga
     6241 cgcgaactga tgagcctgcc gtcggagagc cgacgcgtca caaagtaatt aactaactcc
     6301 tcatgcttag gatagtgccc ggcccttgcg tcgattttga aatgcgcgac ggcttcaagt
     6361 gtcaaacgca cgaattcagg aagcatatac tcaaacaaat ccggccggcc caaatccggt
     6421 gagagcgaga acggctccgc cttcaatcca acggtcgtct tcggcgctgg tgccggcggc
     6481 ggtgcgggcg cgaccggtgc ttccgttgcg ggcgcgggac ttgatggggt tctgcgacgt
     6541 ggccgggacg tgcgagttct gttagaagcg cgttgccttg gcatgggatt gaaccactcg
     6601 gatgtcgagg cttggccggc tggaggcggg cacctccgcc ggccgcttga ttaggcgccc
     6661 ttgcgccgcc gcaaggcaag caccttcgtg ggcctacgct cgcccgagac cagctgcatg
     6721 acgcggtccg cccacaattc catcgctgcc cgcacctgct cggaataggt cgctaggttg
     6781 tacgtgcgcg caacgcggtt gccgacggcg tgaccgagga ccgcctcgat gacgaagggc
     6841 gttgcgatgc cctcattgtt catcgtggtg ctgccgaatc ttctcagatc gtgatgcgtc
     6901 caccctgtga cgccggcacg cgcgtcgagg ttctgcttgg cgcgcgccca cccctgaaag
     6961 ccaccctcgc cggtaccgaa caccagcgtc cggcgcccat cccattgctg gtgacgcgcc
     7021 tgaagaatgc gccgcaccgg tgccgacatc ggaacgtcgc gcgcttcacc ggtcttggta
     7081 cgctcgcccg gaagcctgat cacgccgaca tcgaaatcaa cctcgtcaaa ggccagatgc
     7141 ccaatctcgt tgcgccggca cagcgttaac acaagcaatc tgatgatgct gctgtaatcg
     7201 ccatcgtcca gcgcgcccca tatcttcaca acttcggcct cggtgggaac gtgcgtacgc
     7261 ggtccgttga tcggttgatt ggccatggcg accgtgggag gcgatgtcga taccggtgcc
     7321 gtcgagcagc ccctgcccaa tcgcccagct gaagaacttg ttcaggctgc ccttgacagc
     7381 attggcctcc accggcccgc tgtcggtggc gatcttggcg agcaactccg ccacccgccg
     7441 ccgctcggcc gccttggcaa gctcgagtcg atgcaatggc gccgcgtgct tcatcaggtg
     7501 gcgattgagg ttgatgaagc ttcgcttacg catctctgcc tgcctgcgaa tgaggtaggg
     7561 cttgagcgcc agcgcgaagg tctcgttcgc ccgtttgcgc gcttcggtac gcgtgccggc
     7621 ggggtcctcg gcgaacttgg cgcgagcgta aagctcctcg gccttggcgc gcgccttggc
     7681 gttggtgatt gcggagacgg cgccgatggt gatgcggggt tgacgcttgc cgtgacgata
     7741 gcggaagaag taagtggcgc tgtgctcgcg cagccttatg ccgaagccgg cgatcttgtc
     7801 gtcccagacg gtgtgctcgg gcttgccggc cggcagcgcg agcttggtga cggcgtccct
     7861 agtgagcgtc atgggttcgg tgagcttcat gggaggaccc ttcgtgaacg ccgtgagctt
     7921 ctgctcacgc gtcgcgcctg cggcgattga gcttgtgaat gtcggataca gggagttgat
     7981 aagtgagccg gatgagggtg tcagtgagca gcccccattc gcgactgccc ttgggcgttc
     8041 gcgcgatacg ctcacgaagt tgctgcttta gtctctgtcg ttgctcgtcg gtcatcggag
     8101 gactttcagt tggtggttga gagcagtttc cgtggggagc atatggggag cagccgcgtt
     8161 cgtttccaat cccccttata ccccccttcg agtgatggta tgcgcgaaac ttcaggcgcc
     8221 tacggttttg ttcactcttg ttcggcctca cgggatcagc tgcagacggt catgttcctg
     8281 cagctggtgg cgggcggcca cttgttgctg ttcatcacca ggaacgaacg ctggttcttc
     8341 ctgccgccat tcccggcgct gccgctattc ggagcgatcg tgctcacgca gatcctggcg
     8401 gtactgatgt gcggcttggg ggctggctcg tcccggagat cccctggacg ctgatcggct
     8461 gggtctggct ctacaacatc gcctggatgt tcgtgctcgg cggggtccgg ctgctgaccg
     8521 agcgctttgc cgcctaccgg accgcgcggc agacaaggag catgcagatc gtgaaccagt
     8581 cgcttcggtt gcacgcgaca ccgtaacggg agcattcagg atggattatc gaaagaggtt
     8641 tttggtcgag cccggggcga aggttcggct gtcgaaaatc gaggcgtcct acaccggcaa
     8701 gaacgagacc cacgagaagg cgctccccaa gatccaaaag catgccgagc gcatggacaa
     8761 gctgcagtac ctgctttacg cggacggcag ccaatccctt ctggtggtgc tgcaggcgct
     8821 cgacgcggcc ggcaaggacg gcaccatccg gcacgtgttc agcggaatga atccgcaagg
     8881 gacgttcgtc ttcggattca agcagccgag caaagaggaa cttgcccacg acttcctttg
     8941 gcgcgcgcac ttgcgcaccc cggccaaggg cgaagtggtg atcttcaatc ggtcgcatta
     9001 cgaggatgtg ctcgtggtgc gcgtgcacaa gctcgtgcca cgttcggtgt ggtcgaaacg
     9061 ctacgacctg atcaacgact tcgaaacgat gctgtcgcaa aacggcacga ccattctgaa
     9121 gttctttctc cacatcagcc cggaagagca gctggcgcgc ttcaagcaac ggctcgacga
     9181 cccgtcgcgg cgttggaaga tcagcgaaag tgactattcc gaacgcgagc tgtggccgca
     9241 atacgtggag gcgtacgagg acgccatggc gttgacgagc acgaagcgcg cgccttggta
     9301 catcattccg gcaaaccaca agtggttccg taacctcgcg atatcgcaaa tcatcgccga
     9361 cacgatggac gacatggggt tgaagttgcc gccgacgcgc gtcgacatcg cggatattcg
     9421 ccgcaaatac cacgcggccg cgcgagaacg gaagatcagc gaccaccgtc gggcgaagaa
     9481 agccgacaag gccgcataga cgaaaccgaa tgccgccctg gctactcgcg tcgctgcacg
     9541 gctatcggat cggatggctg ccgcgggatt tgatcgccgg cctgatgctg gctgcgattg
     9601 cgattccggg gcagctggcg accgcgcggc ttgcgggcat gccgccggag accggccttt
     9661 acgcctttgc cgccggctcg ctcgcctttg ccgccttcgg cgccaaccgc ttcatgtcgg
     9721 tggcggccga ttccaccatc gcgccgatct tcgctggcgc ccttgcatcc ttggccgccg
     9781 ccggcacccc ccccctatgc cgagctggtc acgctgctcg cgctgatggt gggcgccatt
     9841 ctggtcgtcg tcggcttgct gcgggccggc tggctcgcaa ccttgctgtc gatccccgtg
     9901 acaacgggct ttctcgcggg catcgccatc cacatcatga tcggcgagct tccgacgctg
     9961 ctcggcatat ccgaggagcg ggggcacatg ctgctgcgtc tctttcacat cctcggccgc
    10021 ctcgacgagg ccaatctcta tacactcgcg ctcggcgccg gcgtgctgat cgtgaccttg
    10081 ggcacggcca ggatcagcgc gaaggttccg ggcgcgctga tcggcgtggt cgccgccggc
    10141 gtcgccgtgg cgctgttcca cctccagagc cgcggcgtga gtctgctcga accgctccac
    10201 gttgagctgc cgcgtctcgc gcttccgtcg ttgcccgaag cgcgcgacat cggccggctt
    10261 ctaccgctcg ccctcatcgt cgccatggtc tgcatcatgc agacgtccgc agtcgcgagc
    10321 acctttcctt cggaggagga tcggccggac gatgccagcc gcgactttgc cggcgtcggg
    10381 gccggcagca tcgtggcagg cctgaccggc tccttccccg tcgat
//
LOCUS       DATLJT010000948         1888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_523527,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000948 DATLJT010000000
VERSION     DATLJT010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523527"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VM910_21745"
     CDS             <1..264
                     /locus_tag="VM910_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVI65180.1"
                     /translation="QGMLFDFHTEQPTSFWMKNTYIPLDMIFIRGDGRILRIAENTVP
                     LSETLVPSGGPVRAVLEVSGGTAKKLGIGPGDRVGHAIFGGGR"
     gene            338..414
                     /locus_tag="VM910_21750"
     tRNA            338..414
                     /locus_tag="VM910_21750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:372..374,aa:Pro,seq:tgg)
     gene            591..752
                     /locus_tag="VM910_21755"
     CDS             591..752
                     /locus_tag="VM910_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65181.1"
                     /translation="MLAHGFTVEQLVELVRAGLAMATVERMVAREKSIEVARVRITEA
                     GRRALSERG"
     gene            1398..>1888
                     /locus_tag="VM910_21760"
     CDS             1398..>1888
                     /locus_tag="VM910_21760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65182.1"
                     /translation="MASTLSLTQSELSAVLWSTHPSVDAMIKRSLLGKRASRSLTRFM
                     IIFGIGVAATLACFGGIVAVALTTSVYTFGQTGSAPVDPGVRGGPAGAGAPLQGLTAD
                     ETAFFQDGQARFADVEVVTKGSNNGLGPRFNSNQCLSCHSQPDAGGSSPAQNPLIAVA
                     TLNG"
BASE COUNT          384 a          574 c          575 g          355 t
ORIGIN      
        1 cagggcatgc tgttcgactt ccacacggaa cagccgacca gcttctggat gaagaacact
       61 tacatcccgc tcgacatgat cttcatccgc ggcgacgggc gcatcctgcg catcgccgag
      121 aacacggtgc cgctctcgga aacgctcgtc ccctccggcg ggccggtgcg cgcggtgctc
      181 gaagtgagcg gcggcaccgc caagaagctc ggcattgggc cgggcgaccg ggtgggacac
      241 gcgatcttcg gcggcggccg gtagctgcgt tgcggtagcc cggcgcggct tgctggcgca
      301 aaccgaaccg tgtatcgagg ccccgacgcg acggcgccgg ggcatagcgc agcctggtag
      361 cgcgggagtt ttgggtactc caggtcgcag gttcaaatcc tgctgccccg accaataaga
      421 taccgaaatc ataagaaaaa gccagagaag gctccgaggc aattctagcg aatcactcga
      481 atcggcacca tgtcggcacg gcacgcaaat tccatccacg tggccccaag cccggccgac
      541 cagcactcga actactcgca tcctcccgcg atggccgcac cgaggcgttg atgctcgcgc
      601 acggcttcac cgtcgagcag ttggtcgagc ttgtgcgcgc cgggctcgcg atggcaaccg
      661 tcgagcgcat ggttgcccga gagaagtcga tcgaggtcgc gcgggtgagg atcaccgagg
      721 cgggacggcg ggcgctgtcg gagcgaggct gatttacatg gcgaactggc gaccgctagc
      781 cgaaaggtat accaaatctg attccaagaa agcactgagc gaactccgaa gacttccacg
      841 aacacgtttt ctgttcgatg aaaacgtaca gaccaacgtg attcgagcaa ttcgctcata
      901 cggtatgcgc gtcgacaccg tgcgggagct aggttagagg cgccgctccg accagaaggt
      961 cttggcattc gcgtggaaac gcaaacagat gctcgtacgc tacgaccgag atttttggga
     1021 cgatgaccgt ttcccgtttg gatcatgcgc gggcgttctt cagttgccgg acattggcaa
     1081 gaacttgaat ttcttctgga gcatcttgac aggccccgtg cggcttttgc gcgcgcgcaa
     1141 acagcgccac gtgggtttca agaagttccg gcgctggaaa cccggagcaa gttccgaaca
     1201 ttaatagtac aacgagacgc gcgaccgatc ggtaggaagg tagtgatcgc gaggcgccag
     1261 gactgctggg gagccaaagc tacctatccg ttcgcaagct tcgccaatct ccgctccgca
     1321 gctgctctcc tcccttcttg ctccagtcac aggatgatgg cctgttgcat caaacaagac
     1381 aggggaggta atcgcagatg gcttccacgc taagcctcac acaatccgag ttatctgcgg
     1441 tcctgtggtc cacccacccg tcggtagacg cgatgatcaa gcgatcgttg ctcggcaagc
     1501 gagcctcgcg cagcctcacc cgcttcatga tcatatttgg cattggtgtc gctgccacct
     1561 tggcttgttt cggcggaatc gtggccgtgg ctttgaccac aagcgtctac acttttggtc
     1621 agaccggatc agctccagta gacccgggcg tgcgcggtgg cccggcgggt gccggcgctc
     1681 cgctgcaggg tcttactgcg gacgaaaccg cgttcttcca ggatgggcag gcgaggttcg
     1741 ccgatgtcga ggtcgtgacc aaaggctcga acaacgggct cggtccccgc ttcaactcca
     1801 accaatgttt gtcttgccac tcgcagccgg acgcaggcgg atcgagcccg gcgcaaaatc
     1861 ccttgatcgc agttgccacc ttgaatgg
//
LOCUS       DATLJT010000949         3758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1212232,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000949 DATLJT010000000
VERSION     DATLJT010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212232"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..356
                     /locus_tag="VM910_21765"
     CDS             135..356
                     /locus_tag="VM910_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65183.1"
                     /translation="MRIASSYCWRIARGITMFDWSSRQYNKTTVRLTREGNYHSAKSV
                     APSLAIELAPSPVENAADIGRDWPIKSVP"
     gene            553..1536
                     /locus_tag="VM910_21770"
     CDS             553..1536
                     /locus_tag="VM910_21770"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65184.1"
                     /translation="MKRRAFITLLGGAAAWPLAARAQQPAMPVIGFIYVGSAATSGSH
                     VAAFREGLNESGYIEGHNVTIAYRWAEGRQDRYPELVADLIRQKVSVIATGANTLASR
                     AAKAATSTIPIVFGVADDPVKLGLVASLASPGGNATGINFFFAELAAKRLGLLREMVP
                     SATRVGVLINPVNATTTESTVKDVQAAAGALGLQIQIFNAGTVREIDSAFASLGRERV
                     DALFVGPDPFFSTRRVQLAVAAARHAVPATYSQRDYIEAGGLMSYGVNVADAYRHIGV
                     YAGRILKGEKPADLPVVQSSKFELVINVQTARTLGLDVPPPLLALADEVIE"
     gene            1629..2534
                     /locus_tag="VM910_21775"
     CDS             1629..2534
                     /locus_tag="VM910_21775"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65185.1"
                     /translation="MRRIGVLMPFTERDPEGQTRVTAFREGLQKLGWTEGRNIRIDYR
                     WASDTTLMPQFAKELVALKPELILASSTPATASILEYTRTTPIVFVQVTDPIGAGFVA
                     SLARPGGNVTGFIAIEPTMAGKWVELLKEIAPRVTRVAAVFNPATAPFPELFLNAFKV
                     AATSFALDAIAAPIHDISELDHVISAQAREPNGGVIVLPDIFIAAHREEVVSLAARHR
                     LPVVYPFRFFTEVGGLLSYGNEPLDQIRRAGSYADRILRGEKPSELPVQAPVKFELLI
                     NLKTAKALALDVPLFLQQRADEVIE"
     gene            2537..3517
                     /locus_tag="VM910_21780"
     CDS             2537..3517
                     /locus_tag="VM910_21780"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65186.1"
                     /translation="MKRREVITLLGGAAAAWPALANAQQTAKPPTIGYLGPNSRLLDG
                     QRLSAFVQRLRELGWIEDRTITIEYRWADGRNEHLADIAADFVRRKVDVIVTSATPPT
                     VAAKQATSVIPIVFAAVGDPVGSGVVKSLARPGGNATGLSLQATDAVGKRLQLLGEVI
                     TGLRRLAIMANSGNPSAALEMREAEATARTLGLEGVTSEIRRSEDIAPAFDAIKGRVE
                     ALYVCNDPLATTNRVRISTLASAARLPTMFIAREYVEAGGLMSYGANFQDLYRRTAEF
                     VDKILRGTKPDDIPVEQPTKFDLIVNLTTAKALGLEIPPTLLARADEVIE"
BASE COUNT          735 a         1168 c         1133 g          722 t
ORIGIN      
        1 cataaaactt cgcggtcggg cagaccgtga aataggtgat gatcatcgct gcgccactag
       61 cgtgatcttc cccccgttgc ggggcaccgt gcgcttgggt catggcaagc aacaacgcaa
      121 gcgtggcggc aatgttgcgc atcgcatcct cctattgttg gcgaattgct cgcggcatca
      181 cgatgttcga ttggagttcc cggcaataca acaagaccac cgttcggctc acgcgcgaag
      241 gaaactatca ctcggccaag tccgtggcgc cctcacttgc gatcgagttg gcgcccagtc
      301 cggtcgagaa tgctgccgat atcggtcgag actggccgat taaatcggtg ccgtaagaca
      361 tcagaccacc tgccacgaca aacgaacgga agtagtagac cgccggtaag cggtaccgag
      421 cggcgccaaa gctgctgacc tgccggtgca ggcgccaacc aagtacgaaa cgaccctcaa
      481 cctcaagacg gcgaaggcgc tcggtttcga cgtgccgcct tcgctactcg tgcgcgccga
      541 cgaggtgatc gaatgaagcg ccgcgcgttc atcacgctcc tcggcggcgc agccgcgtgg
      601 ccgctcgcgg cgcgcgcgca gcagccggcg atgccagtga tcgggttcat ctacgtcggg
      661 tcggccgcaa catccggctc ccatgtggcc gcctttcggg aaggcctgaa tgaatcgggt
      721 tatatcgagg gccacaacgt gacgatcgca taccgctggg cggaaggccg ccaggatcgg
      781 tatccggagc tggtggccga tctaatccgc cagaaggtga gtgtgattgc cacgggcgcc
      841 aacacgctgg cctcgcgcgc ggccaaggct gcgacctcga cgatcccaat cgtcttcggt
      901 gttgccgacg acccggtgaa gctcggcttg gtggcgagcc tcgccagtcc ggggggcaac
      961 gcgacgggca ttaatttttt ctttgccgag ctggcggcaa agcggctcgg actgctgcgc
     1021 gaaatggtgc cgagcgcgac ccgcgtgggc gttctcatca atccggtcaa cgcgaccacc
     1081 accgagtcca cggtgaaaga tgtgcaggcg gctgcgggcg ccctcgggct gcaaattcag
     1141 atcttcaatg ccggcaccgt tcgcgagatc gattcagctt ttgcaagcct agggcgcgag
     1201 cgcgtcgatg ccctcttcgt cggccccgac ccgtttttca gcacccggcg cgtgcagctt
     1261 gccgtagcgg cggcgcgcca cgccgtccct gcgacctatt cacagcgcga ctacatcgaa
     1321 gctggcgggc tgatgagcta cggagtcaac gtcgcggatg cttatcgtca catcggcgtc
     1381 tatgccggcc gcatcctcaa gggcgagaag ccggcggacc tgccggtcgt gcagtcaagc
     1441 aagttcgagc ttgttatcaa cgtgcagacc gcccgcacgc tcggccttga tgtgccgccg
     1501 ccgctgctcg cgctcgccga cgaggtgatt gaatgactgc gaagatgaag cggcgcgact
     1561 tcatcacgct catcggcggc acggcggcgg cgtggccgct cgcggcgcgt gcgcagcagc
     1621 gcgagcgcat gcggcggatc ggcgtgctga tgccgtttac cgagcgggat ccggaaggac
     1681 agacgcgcgt cactgcattc cgtgaaggtc ttcagaaact cggctggaca gagggccgca
     1741 atatccggat cgattaccgc tgggcgtccg atacgactct aatgccgcaa tttgccaagg
     1801 aactcgtcgc gctgaaacct gagctcattc ttgcgagcag cacaccggca accgcgtcga
     1861 tactggaata cacgcgcacc acccccattg ttttcgtgca agtaaccgat ccgatcggcg
     1921 ctggcttcgt cgcgagcctt gcacggccag gcggtaatgt cactgggttc atcgctatcg
     1981 agccgacgat ggccggcaag tgggtggagc tgctcaaaga gatcgcgccg cgtgtcacgc
     2041 gtgtcgcagc tgtgtttaac cctgcaaccg caccgtttcc cgaacttttc ctgaatgcct
     2101 ttaaagtcgc tgccacctcc ttcgcgctag acgcgatcgc cgcacctatt cacgatatct
     2161 cagagcttga tcatgtaatt tcggcacagg cacgcgagcc gaatggtgga gttatcgtac
     2221 tgccggatat cttcatagcc gcccatcgcg aagaagttgt atcgctggcg gctcgtcacc
     2281 gtctgcctgt cgtctatccc tttcgctttt tcacggaagt cggcggcctg ctgtcttacg
     2341 gaaacgaacc gctcgatcaa attcggcgcg caggcagtta cgctgatcgt atcctgcggg
     2401 gcgagaagcc gagcgagctt cccgttcagg ctccagtcaa gttcgaactg ttgatcaatc
     2461 tcaagacagc caaagcgctc gcgctcgacg tgcccttgtt tctacagcag cgcgcagacg
     2521 aggtgatcga gtgaagatga agcggcgcga ggtcatcacg ctactcggcg gcgctgcggc
     2581 ggcgtggccg gctctggcaa acgctcaaca gaccgcaaaa ccgccgacca ttggttattt
     2641 aggccccaac tcgcgtttgc tcgatggcca acgtctttcc gcctttgtac agcgactacg
     2701 cgagctcggc tggatcgagg accgcaccat cactatcgaa taccggtggg cggacggacg
     2761 caatgagcat ctcgccgata tcgcggccga cttcgtccgg cgcaaggtgg acgtcattgt
     2821 tacgtccgca acgccaccca ccgttgcggc aaagcaagcg acatccgtga tcccaatcgt
     2881 gttcgcggcg gtcggtgacc cggtcgggtc gggcgtggtt aagtccttgg cgcgaccggg
     2941 cggcaacgcc accggcctct cgctacaggc aacggatgct gtcggtaagc gacttcagct
     3001 acttggcgaa gtgatcacag gtctgcgccg gttggcgatc atggccaata gcggtaatcc
     3061 ctccgccgct ctggaaatgc gcgaggctga ggccactgcc cgcacgctgg gtctcgaagg
     3121 agttacatca gaaatccgtc gatccgaaga catcgcgcct gcgttcgatg cgattaaagg
     3181 ccgcgtggag gccctttacg tctgcaacga tccactcgca accaccaacc gggttcgcat
     3241 cagcactttg gcatcggccg cgcggctgcc gaccatgttc atcgctcgcg agtatgtcga
     3301 ggcgggaggg ctgatgtcct atggagctaa cttccaggac ctctaccgac gcaccgccga
     3361 atttgtagac aagattttgc ggggaacgaa gccggacgac atcccggttg agcagccgac
     3421 caagttcgat ctcatcgtta acctgaccac tgcgaaggcg ctcggccttg aaattccgcc
     3481 gacgctgctc gcgcgcgccg acgaggtgat cgagtgagtc gcgaacgaag gatgaatcca
     3541 aagtgtctgg gacgaccatt cccagacact ttcagtgcaa gaagtgccca tttgacgggg
     3601 ctgaaaatga cccgaataac tcgcgccgat ttatcctcac cgatgcctcg accagcattg
     3661 cttttttcgc gttgggtctc aaaggcgcgc tacgatgtta ggcgcgcacc accattatgg
     3721 gacattgcgc ctcacgcaag atgtccttta gggtcaac
//
LOCUS       DATLJT010000950         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2360320,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000950 DATLJT010000000
VERSION     DATLJT010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360320"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1124)
                     /locus_tag="VM910_21785"
     CDS             complement(150..1124)
                     /locus_tag="VM910_21785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65187.1"
                     /translation="MKRREFITLLGGAAAWPLGARAQQAGKKMLRVGTVSALQQSSPT
                     WAAFMRRMAELGYQEGKTFVFDLVSATSEEDYETGYRTLAARKPDIIMATGPESALKS
                     ALAVTRTLPIVMIAIDYDPFARGYVTNLARPSGNVTGVVFQQIELAAKRIQLIKDAFP
                     DRPAATMFWDRLSADQWQAARSAAATLGLQLSGIELRELPYDYDASLSQAPLDHRGML
                     IVPTSPFFFRDRARLADFALRHRILSMFVFREWVDAGGLLCYGPSITALFERVAEFVD
                     RIARGAKPADLPIEQPTKFEMVVNLKTARTIGIDLPTAILLRADEVIE"
     gene            complement(1121..2104)
                     /locus_tag="VM910_21790"
     CDS             complement(1121..2104)
                     /locus_tag="VM910_21790"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65188.1"
                     /translation="MRRRDFITLLCGATAAWPLAARAQQTAMPVVGYLSARSPQDTTH
                     LVEAFGRGLAEAGFVEGQNVLIEYRWAFGQHDRLPGLAADLVRNPLTVLVTTGGEVAA
                     LAAKAATSTIPIAFIVGTDPVKLGLAASYNRPGGNATGINILTNSLEPKRLELLRELV
                     PQASAIGALFDPSFLAYEDQFRDVREAARALDLQVYELRASTDAEIDLAYETVARQRI
                     AAVTVAAGPFFDTRRDRLVALAARHAVATMYHFREFPAAGGLMSYGVDNRVTYRQIGV
                     YAGGILKGAKPAELPILQPTKFELVLNLKTAKALGLKVPLTLQVAADEVIE"
     gene            complement(2332..>2725)
                     /locus_tag="VM910_21795"
     CDS             complement(2332..>2725)
                     /locus_tag="VM910_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65189.1"
                     /translation="DGDGRYDVLEVETRGPFKGPRAYDATGLPLHFDNQSIFKERFHL
                     DKADPNILHDEITVIDHALTRPWTVDKRYGRDPNPRPNWPESYCTENNAQIVIGKENY
                     FLSADGLLMPAKRDQPPPDLRYFKQTQR"
BASE COUNT          539 a          827 c          836 g          523 t
ORIGIN      
        1 gtaccacccg ccgccacgat ttcgtgcctt ggcgcttttc aaccgccggc cggcagagca
       61 cgggattaga tcatcgctgc cggcggtcga aaacctgcac aataccggct gtgagcaggt
      121 gcagcaaaac ctcggcgcag aaaccagggt tactcgatca cctcgtcggc gcgcagcaag
      181 atcgctgtcg gcagatcgat accgatcgtc ctggcggtct tcaggttcac caccatctcg
      241 aactttgtcg gctgctcgat aggcaaatcg gcgggcttgg cgccgcgggc gatccgatca
      301 acgaactcgg caacgcgctc gaagagcgct gtgatactcg gcccatagca caacaggccg
      361 cccgcatcta cccattcgcg gaagacaaac atggatagga tccgatgccg aagcgcgaag
      421 tcggcaaggc gcgcgcgatc gcgaaaaaag aacggcgacg ttggcactat gagcatgccg
      481 cgatgatcga gcggtgcctg actgagcgac gcgtcatagt catagggcaa ctcgcgcagt
      541 tcaattccag aaagctgcaa gccgagcgtg gcggcggcac tccgcgctgc ttgccattga
      601 tccgcagaca agcggtccca gaacatggtc gcagccggcc tgtctggaaa ggcatccttg
      661 atgagctgaa tccgctttgc tgccagttcg atctgctgga agaccacgcc ggtgacattg
      721 ccggagggcc gcgccaggtt ggtcacgtag ccgcgagcga acggatcgta gtcgatagcg
      781 atcatcacga tcggtagcgt tcgcgtgacg gcaagcgccg acttcagggc actttccggg
      841 ccggtggcca tgatgatatc gggtttgcgc gcggcgagcg ttcggtagcc ggtttcgtaa
      901 tcctcttcgc tagtggccga cactaaatca aagacgaaag tctttccttc ctgataaccg
      961 agttcagcca tgcgccgcat gaaagcggcc catgtcggcg aagattgctg gagggcggag
     1021 acagtgccga cgcgcagcat cttcttgccc gcctgctgcg cgcgcgcgcc gagcggccat
     1081 gccgccgcac cgccaagcag cgtgatgaac tcgcggcgct tcattcgatc acctcgtcgg
     1141 cggccacttg cagcgtgagc ggcactttta ggccaagcgc ctttgcggtt ttaagattga
     1201 ggactagctc gaacttggtc ggctgaagga tgggcaactc tgctggcttt gcgcccttga
     1261 gaatgccgcc agcgtagact ccaatctgac gataagtgac gcgattatcg accccgtagc
     1321 tcatgagccc gcctgccgca ggaaattcgc ggaaatggta catcgtcgcc accgcatggc
     1381 gggccgccaa ggccacaagc ctgtcgcgcc gcgtatcaaa aaacggtccg gcggcgaccg
     1441 tcaccgctgc gatgcgctgc cgcgcaactg tttcataggc caggtcgatc tctgcgtccg
     1501 tgctggctcg caattcgtag acttgcaggt cgagcgcccg cgccgcctcg cgtacgtcgc
     1561 ggaactggtc ctcgtaggcc aggaagctcg ggtcgaacaa ggcgccgatc gcggaggcct
     1621 gcggcaccag ctcacgcaac agttcgagcc gcttgggctc cagggagttg gtcaagatat
     1681 tgatcccggt cgcgttgccg cccggccggt tgtagcttgc tgcgagcccc agcttgactg
     1741 gatcggtgcc tacgatgaag gcgatgggga tcgtcgacgt cgcggccttg gctgccagcg
     1801 ccgccacctc tccccccgta gtaacgagaa cagtcagggg attgcgaacg aggtcggcgg
     1861 cgagtcccgg cagtcggtcg tgctgaccga aggcccagcg gtattcgata agcacgttct
     1921 gaccctcgac gaagccggct tcggctaggc cgcgtccgaa cgcctccaca agatgtgtag
     1981 tgtcctgggg cgacctcgcg ctgagatacc cgaccaccgg catcgctgtc tgctgcgcgc
     2041 gtgcggcgag cggccacgcg gctgtcgcgc cgcagagtag agtgatgaaa tcgcgccgcc
     2101 tcatggaggc ttaccccagg gccaggatca cggaggacta taacaggtct gaactgggtc
     2161 ggtggcgtgc atcgcaacaa aaaaggtcgc cctatgaccg ctttgggtca caagcggaaa
     2221 gcctcagatt gagcaaatct tgtccgcttt gccccccaaa acggacatgt gcgagctagc
     2281 ctcgattgtc ggagccattc tgattatcaa cacaagcgtt ccgccggtgt gtcacctctg
     2341 cgtttgcttg aaatatctca aatccggcgg cggctggtcc cttttggcag gcatcaacaa
     2401 gccatcggcg ctcaggaaat agttttcttt gccaatgacg atctgtgcgt tgttctcggt
     2461 gcaataagat tccggccagt tcggacgcgg attcgggtcg cgcccgtatc gtttgtccac
     2521 cgtccagggc cgcgtcaagg catgatcgat cacggtgatt tcgtcatgaa gaatgtttgg
     2581 atcagccttg tcgagatgaa accgctcctt gaaaatcgac tgattgtcga agtgcagagg
     2641 tagcccagtt gcgtcgtaag cgcggggacc tttaaacggg ccgcgcgtct cgacctcgag
     2701 cacgtcgtag cgaccatcgc cgtcc
//
LOCUS       DATLJT010000951         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2360349,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000951 DATLJT010000000
VERSION     DATLJT010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360349"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VM910_21800"
     CDS             complement(<1..1083)
                     /locus_tag="VM910_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI65190.1"
                     /translation="MAFPLNIRSMAVRYRLNTIAASLALVFGSSLPLNAQSKAPEVMV
                     ANPVLKRIVQWDEYTGQFEAVRRVEVRARVSGELVKIHFTDGQTVKAGDILFTIDPRP
                     FEIAVEAARAEVARARAQIAVTGNDLERAEQLTPSKVLTQRDFDQRKANHDAAKAQLQ
                     SAEAMLRNAELNLEWTSVLAPIDGRISDRKVDVGNLVSGGQVGASLLTTIVSLHPIHF
                     VFNVSEPDYVRYSRLAANGQRLSSSVPGNVVQIRLADEKEWIRNGRMNFIDNQLNGKS
                     GTIRARAVVDNEDFFLTPGTFGRLRLFGGNVEALLIPDAAVTSDQSRKVVYTVGPDNK
                     IQVKPVTLGPIEENLRVITGGLSKDDR"
     gene            complement(1283..1765)
                     /locus_tag="VM910_21805"
     CDS             complement(1283..1765)
                     /locus_tag="VM910_21805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65191.1"
                     /translation="MKEPTEKGQKTWVLALTSVASLMVALDALVVTTALSTIIETLQR
                     TMNAYNLSFAVLLLTGAALGDRFGRRRTFVAGLGLFVAASAACVLAGSAGWLIAARAV
                     QGAGAALVMPLAIALLSAAFPPHVARQGARDLQWLDRAGSLNPLGTRGFGASEGERLR
                     "
     gene            complement(1822..2562)
                     /locus_tag="VM910_21810"
     CDS             complement(1822..2562)
                     /locus_tag="VM910_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVI65192.1"
                     /translation="MRSHPIVSQDKWLAARARLLAKEKEFTRQRDQLSEERRALPWVK
                     VEKEYVFDGSNGRETLSQLFQDRSQLLIYHFMYGTDWNEGCKNCSFWADSFNGITAHL
                     KHRDVTMIVMSRAPYSKLKTFQNRMGWSFKWVSSSTTDFSRDFNVSFTPEEIKGTVFY
                     NYEHRKFPMTEAPGISVFYKDDAGTIFHTYSCYGRGLDAVNCAYQLLDLVPKGRNEAD
                     LPNPMSWVRHHDKYDDSPFETSEARLSV"
     gene            3066..>3553
                     /locus_tag="VM910_21815"
     CDS             3066..>3553
                     /locus_tag="VM910_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVI65193.1"
                     /translation="MTAFTAGKPLDREAFDRLLGELRPKLHRYCARMTGSVLDGEDVV
                     HEAIVKALEASSQTGSIAHPEAWLFRIAHNAALDFLRHRARQDAAHADEDPDMIIDPL
                     AVAEDRQATAASLQTFMRLPVAQRGSVILMDVLGYSLEEIGSVMDVSIATVKAALHRG
                     RAR"
BASE COUNT          688 a         1097 c         1026 g          742 t
ORIGIN      
        1 ccggtcatcc ttggacaagc cgcccgtgat gacgcgaaga ttttcctcga ttggtcccag
       61 tgtcaccggc ttgacttgaa tcttgttatc aggcccaacc gtataaacga ctttgcgact
      121 ctgatccgac gtcacggcgg cgtccgggat gagcagggct tcgacgttgc caccgaaaag
      181 gcgcagacgc ccaaatgtcc cgggcgtcaa gaagaaatcc tcattgtcca ccacggcccg
      241 agcgcgaatg gtgcccgact tgccgttgag ctggttgtcg atgaagttca tccgtccgtt
      301 tcgtatccac tctttctcat ctgctagacg gatctgaacg acgttgccgg gcacgcttga
      361 cgacagtcgc tggccgttcg ccgccagacg cgagtagcgc acgtagtccg gttccgagac
      421 gttaaacacg aaatggatcg ggtggaggct gacgatggtc gttagcagcg aagcaccgac
      481 ttgcccgccg gagacgaggt tgccgacgtc gacctttcga tcggagatgc ggccatcgat
      541 tggcgcgagt accgatgtcc attcaagatt gagctccgcg ttgcgaagca tggcctcggc
      601 ggattgcaac tgcgccttgg ccgcatcgtg gttggctttc cgctggtcga agtcgcgctg
      661 cgtgagaact tttgacggcg tcagctgctc ggcgcgctca aggtcattgc ccgttaccgc
      721 gatttgcgct ctcgcgcgag cgacctccgc acgggctgcc tccaccgcaa tctcgaacgg
      781 tctgggatcg atggtaaaaa gtatatcccc cgccttcacc gtctggccgt ccgtgaaatg
      841 gattttcacc agttccccgg agacgcgcgc gcgtacttcg acccgccgca cggcctcgaa
      901 ttgaccggtg tattcgtccc actgaacgat tctttttaga actggatttg cgaccatgac
      961 ctccggtgct ttcgattgcg cgttcaatgg caaggagctg ccaaacacca gcgctaacga
     1021 ggccgcaatg gtgttcagcc gatagcgcac cgccatcgat cgaatattga gaggaaacgc
     1081 catctcattt tcctccttca gcgcggtctc aagggcgttg aacgtcgaag tgcgctgaca
     1141 gtcgctgagc gctcgcttcc acggattcga cgtaagcctt gaacgatttg agcgccgtat
     1201 cccagaactg gtgcttcgcc tctcggtcaa cgggacacac gattcgttcg tgcggcatga
     1261 gtgcaccttg gtgccaaccg gcttatctca ggcgctcgcc ttcacttgcg ccaaagccgc
     1321 gcgtgccgag cgggttgaga gagccagccc tgtcaagcca ctgaagatcc cgagcgcctt
     1381 gccgcgctac gtgcggcgga aatgccgcgc tcaagagcgc aatggcgagc ggcatcacca
     1441 gcgccgcgcc ggctccctgg acggcgcgcg cggcgatcag ccagcccgcg ctccctgcca
     1501 gcacgcatgc agccgacgcc gccacgaaaa ggccgaggcc ggcgacgaag gtgcgccggc
     1561 ggccgaagcg gtcgccgagc gcggcgccgg tcaagagcag taccgcaaag ctcagattgt
     1621 aggcattcat tgtccgctgt agcgtctcga tgatcgtact gagggctgtt gtaacgacca
     1681 gcgcgtcgag tgcgaccatc aacgacgcca ccgaggtcag tgcaagcacc caggtcttct
     1741 gccctttttc ggtcggctct ttcattattt tgctactttc cttgctgcgg gcgttggccc
     1801 cggtttaccc atcgacttgc gtcagacgga aagtctcgct tcggacgtct cgaatggtga
     1861 gtcgtcgtat ttgtcgtgat ggcgcaccca cgacatcgga ttcgggagat cagcttcatt
     1921 gcgacccttg ggcaccaggt cgagcagctg ataagcgcag ttcacggcat ccagcccacg
     1981 gccgtaacac gagtacgtgt ggaagatcgt acccgcgtcg tccttgtaga agacgctgat
     2041 ccccggtgcc tcggtcattg gaaatttacg gtgctcgtag ttgtagaaga ccgttccctt
     2101 aatctcctcg ggcgtaaatg agacgttgaa gtcgcgactg aaatcggtag tgcttgacga
     2161 tacccacttg aagctccagc ccatccggtt ctgaaaggtc ttcagtttcg aatacggcgc
     2221 acgcgacatc acgatcatcg tgacgtcgcg atgtttcaga tgcgccgtga tgccattgaa
     2281 gctgtctgcc cagaacgagc agttcttgca gccctcgttc caatccgttc cgtacatgaa
     2341 gtgatagatc agcaactggc tacgatcttg gaaaagctgc gaaagggtct cacggccgtt
     2401 cgacccgtcg aagacatact ccttctccac cttcacccac ggtagcgcgc ggcgctcctc
     2461 gctcagttga tcgcgctgcc gggtaaactc cttctctttg gccagaagcc gcgcacgggc
     2521 agcgagccat ttatcttgcg aaacgatcgg atgactcctc atggcaacct cacgcgtcag
     2581 cgttgcggcg taattgccgg cctttagtac gcattccagt gttgaggccg gtagatcgtt
     2641 tccggggaga tcggcgcgac cgaatcgtcc tcacaattcc acggagtcgc ccgtgtcgtg
     2701 atcgctagga tggtcaccgc catcgcaaca atcatcccga gccaagcctg ttgcgaaacg
     2761 atcggatgat tcctcatgac aacttcacac gtcagcgttg cggcggaatt cgccctcacc
     2821 tgggaattgg acgcaacttc ccacatgggg tccggtcctg aagtctccca agaggaggct
     2881 tcccggcgac aaagcgaggc actcgcgttc ctcgtgggct cgcagcgaat ttgcgtcgcc
     2941 acttgcctac gaccgagaga cgaatggggg tatccaaaag attctcagga aaaatttttt
     3001 attctgccga atccttgggc cgatcccgga cgtcctatat ccaggtgacc actctggagt
     3061 ggtccatgac tgcctttacg gctggcaagc ccctggatcg cgaggccttc gaccggctac
     3121 tcggcgaact gcggccaaag ctgcatcgtt actgcgcgcg tatgactggc tcggtccttg
     3181 acggcgagga tgtggtccac gaggcgatcg tcaaagcgct ggaggcatca tcccagacgg
     3241 gatcgatcgc gcatccagag gcctggctgt ttcgcatcgc tcacaatgcg gcactggatt
     3301 ttctgcggca ccgcgcccga caggacgccg cccacgctga cgaagacccg gacatgatca
     3361 tcgacccgct agccgtcgcg gaagatcggc aggccacggc cgcgagcctc cagaccttca
     3421 tgcgccttcc tgtggcgcag cgcggcagcg tcatcctcat ggacgtgctc ggctattccc
     3481 tcgaggaaat cggcagcgtc atggatgtga gcattgcgac cgtgaaggcg gccctgcatc
     3541 gcggccgcgc ccg
//
LOCUS       DATLJT010000952         3815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2591800,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000952 DATLJT010000000
VERSION     DATLJT010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591800"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VM910_21820"
     CDS             <1..1030
                     /locus_tag="VM910_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HVI65194.1"
                     /translation="MFFSEMSGSTTADVAVMSRALGGPMRREGYESAFIAAIIAAAST
                     IAALVPPSITAVVYGAVGNVSIAGLFMAGVVPGFMIGFGLMIYCYFFGPSGMRKPRAS
                     LRQVAFATADAALPLMIPVILLGGILTGWFTPTEAGVVAVIWILVVVIPALNRGHLKN
                     IPYDFCLAGLIFSLPLITIGAANAFGWMLAYLRGAIVIADWITSIAGNDPHYIMLLMV
                     LLFTVVGDFIEPVPTIIIFMPLVNALTQAGDINGVHMGVVLIATLAFGLITPPYGLVL
                     LMASKFVGISFGKALRAALPIYVVFLITITFTIYFPSVVLWLPKHVIPESVGCFKSPA
                     GTGYICPN"
     gene            complement(1183..1989)
                     /locus_tag="VM910_21825"
     CDS             complement(1183..1989)
                     /locus_tag="VM910_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI65195.1"
                     /translation="MSASAPASVSSPDDPALLRVDGPIATITLNRPAAFNSIDLSIAR
                     KLEQLGAKVEASDEIKVLVIEGEGRAFCAGGDLQTIGAAAASDTIAPVVGEMLKHYHA
                     FITTLRRMPKVVLASVHGSAAGAGLSLAFVADLCIAAQDAKFTPAYSKLGVSPDGGGT
                     VGVVASVGPRRALQIYLAEDSFSADQAYQWGLVAKVVPAAELKAATRELALRLAQNAP
                     AGLAATKALIHRSPTTPIEQQLDAERDAIIGCMHTEEFRVAVKKFTSKGK"
     gene            2166..3362
                     /locus_tag="VM910_21830"
     CDS             2166..3362
                     /locus_tag="VM910_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI65196.1"
                     /translation="MDTVVERPVSKIDPFSHAFLENPYPHHDSLREAGPVVWLEHYRI
                     WAMARHQEVRDTLTDWQTYCSGAGVGLSDFRKEPPWRPPSIILEADPPLHTRTRAVLT
                     RIMSPAAINVLQTTFDREAEVLVDRLVDKREFDGIADFAEAFPLKVFPDAVGIADEGR
                     ENLMRYGNMVFNSFGPRNDLLAKAMENAGPVRDWIMSKCSRAALAPDGLGMQIFQAVD
                     SGELAEAEAGMLVRSFLSAGIDTTVYGLGNALYCFVRHPEQWTMLRENPSLIRLAFEE
                     VLRFEAPVQTFFRTTTREVEVAGVRLGEGEKVLLFLAAANRDPRRWERPDQFDIRRRA
                     TGHMTFGTGIHGCVGQAVARLESEALFGALARRVASFEMIGEPTRRLNNTLRGLDALS
                     LRLILA"
     gene            3546..>3815
                     /locus_tag="VM910_21835"
     CDS             3546..>3815
                     /locus_tag="VM910_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI65197.1"
                     /translation="MTQGPVLIVGAGHGGFQVALSLRQLGFKERICLINDEAHLPYQR
                     PPLSKAYLKGSGGPETLMFRPEKFYADQRIELIGDRAVAIDRAARK"
BASE COUNT          647 a         1185 c         1246 g          737 t
ORIGIN      
        1 catgttcttc tcggaaatgt cgggctcgac caccgccgat gtcgccgtga tgagccgcgc
       61 gctcggcgga ccgatgcggc gcgagggtta tgagtccgcc ttcatcgccg cgatcatcgc
      121 ggcggcctcg accatcgcgg cgctggtgcc gccgagcatt accgccgtgg tttacggcgc
      181 ggtcggcaac gtttcgatcg cgggcctgtt catggcgggc gtcgtgccgg gcttcatgat
      241 cggcttcggg ctgatgatct attgctattt ctttgggccg tcgggcatgc gcaagccgcg
      301 cgcctcgctg cgtcaggtgg ccttcgcgac ggcggacgcc gcgctgcctt tgatgattcc
      361 ggtgatcctg ctcggcggca tcctgaccgg ctggttcacg ccgacggaag cgggcgtcgt
      421 cgcggtcatc tggattcttg tggtcgtgat ccccgcgctc aaccgcggac atctgaagaa
      481 catcccttac gacttctgcc ttgccggact gatcttctcg ctgccgctga ttacgattgg
      541 tgccgccaat gcgttcggct ggatgctcgc ttacctgcgc ggcgccatcg tcattgccga
      601 ctggatcacg tcgattgccg gcaacgatcc tcattacatc atgctgttga tggtgctgtt
      661 gtttaccgtg gtcggcgact tcatcgaacc ggtgccgacc atcatcatct tcatgccgct
      721 ggtgaatgcg ctgacgcagg ccggcgacat caacggcgtg catatgggtg tggttctgat
      781 cgcgaccctc gcgttcggcc tgatcacgcc gccctacggg ctcgtgctgc ttatggcctc
      841 gaaattcgtc ggcatcagtt tcggcaaggc gctgcgcgcg gcgctgccga tctatgtggt
      901 gttcctgatc accatcacct ttacgatcta tttcccgagc gtggtgttgt ggctgccgaa
      961 acacgtgatc ccggagtcgg tcggctgttt caagtcgccg gcgggaacgg gatatatctg
     1021 tccgaattga ttccctcgtc gtcccggcca agcgagcatc gcgagcgcga gccgggaccc
     1081 atagccacag gtcagagttt cgcgaaggac gtcgacgagc tcagtatcac agatcgaatc
     1141 cgcggtatgg gtccccgctt tcgcggggac gacggcgagg ttttacttcc ccttgctcgt
     1201 gaacttcttc acggcaacac gaaactcttc cgtgtgcatg caaccgatga tggcgtcgcg
     1261 ttcggcgtcg agctgctgct cgatcggcgt tgtcggagaa cggtggatca gcgccttggt
     1321 cgctgccagt cccgccggcg cgttttgtgc aaggcgcagc gcgagctcgc gcgtcgcggc
     1381 cttcagttcg gccgccggga ccaccttcgc aacaagtccc cattgataag cctggtccgc
     1441 ggaaaagctg tcctcggcga gatagatctg cagcgcgcgc cgcgggccga cgctcgcgac
     1501 cacgccgacc gtgccgccgc cgtcgggcga gacgccgagc ttggaatagg ccggcgtaaa
     1561 cttcgcatcc tgggcggcaa tgcaaagatc ggcaacgaag gcaagcgaga ggccggcacc
     1621 ggccgccgag ccgtgcacgc tcgccagcac gactttgggc atccgccgca acgtcgtgat
     1681 gaaggcgtgg tagtgtttca gcatctcgcc caccacaggc gcgatcgtgt cgctggctgc
     1741 ggccgcgcca atggtctgca ggtcgccgcc cgcgcaaaaa gcgcggcctt cgccttcgat
     1801 gaccagcacc ttgatctcgt cggaggcctc gacttttgcg ccgagctgct cgagcttcct
     1861 ggcaatcgac agatcgatcg aattgaaggc cgcagggcga ttgagcgtga tcgttgcgat
     1921 cgggccgtcg acgcggagca gcgcaggatc gtcgggcgac gagacggagg caggagcgga
     1981 ggctgacatt ggaaactccc ggcatggaga aggctgaccg gcatttaaat cgcgcaaagg
     2041 ccgggtgaca atcccccgca aagcttgcaa gatgaactgg cggcgagacg gggcgaagac
     2101 gccgcccctc agggtatcaa gcgcaaacgc gcaggcaata tgagcgggag gaggcgctgg
     2161 ctcacatgga cacggtcgtc gagcgtcccg tttctaaaat cgatccgttt tcgcacgcgt
     2221 ttctggaaaa tccctatccg caccacgaca gcttgcgtga agcggggcct gtggtgtggc
     2281 tcgagcacta tcgcatctgg gcgatggcgc ggcatcagga ggtgcgcgac acgctgacgg
     2341 actggcagac ctattgctcg ggcgcgggcg tgggcctgag cgatttccgg aaagagccgc
     2401 cgtggcggcc gccgagcatc atcctggagg cggacccgcc gctgcatacc cgcacccgcg
     2461 cagtgctgac ccgcatcatg tcgccggcgg cgatcaacgt cttgcagacg acgttcgacc
     2521 gcgaggccga ggtgcttgtc gaccggctgg tcgacaagcg cgaattcgac ggcattgccg
     2581 atttcgccga agcgtttccg ctcaaggtct tcccggatgc ggtcgggatc gcggatgagg
     2641 gccgcgagaa cctgatgcgc tacggaaaca tggtgttcaa ttcattcgga ccgcgcaacg
     2701 acctgctcgc aaaggccatg gagaatgccg gcccggtgcg ggactggatc atgtcgaaat
     2761 gcagccgcgc cgcgctcgca cccgatggtc tcggcatgca gatcttccag gccgtcgatt
     2821 ccggcgaact ggcggaagcg gaagccggta tgctcgtacg ctcgttcctg tcggccggca
     2881 tcgacaccac ggtctacggg cttggcaacg cgctctattg ttttgtgcga caccccgagc
     2941 aatggacgat gttacgcgaa aatccgtcgc tgatccgtct tgccttcgag gaagtgctgc
     3001 ggtttgaagc cccggtgcag acgttcttcc ggaccacgac cagggaggtc gaggtcgcgg
     3061 gcgtccgact gggcgagggc gagaaggtgc tgctgttcct tgctgccgcg aaccgggacc
     3121 cgcgccgctg ggaacggccg gatcaattcg acatccgccg tcgcgcgacc ggtcacatga
     3181 ccttcggcac aggtattcac ggctgcgtcg ggcaggcggt ggcgcggctc gagagcgaag
     3241 cgttgttcgg cgcgctcgca aggcgcgttg cgtcgttcga gatgatcggc gaaccgactc
     3301 ggcggctcaa caacacgctc cgcgggctcg atgcgttgtc gcttcggctg atcctggcat
     3361 aacgcatacg cgaactcgcg accggacgcg gtacccttgc gccgtgtcgg gccatcgggc
     3421 taaatggcgc cttcatcggc ggggcctcgg acacgagcgc cgctcttcgc ggacgatcgc
     3481 aagccaaatc ggtgaagagg tgacgacatg ggtaggtccc atgccctatc gtttgggctg
     3541 tcgcgatgac gcaaggtccg gttctgatcg ttggcgccgg ccatggcgga tttcaggttg
     3601 cgctttccct gcgccaactc ggcttcaagg aacgcatctg cctgatcaat gacgaggcgc
     3661 atctgccata tcagcggccg cccttgtcga aggcctatct gaaaggcagc ggcggaccgg
     3721 aaacactgat gttccggccc gagaaattct acgccgatca gcgcatcgag ttgatcggcg
     3781 accgcgcggt tgcgatcgac cgcgccgcgc gcaag
//
LOCUS       DATLJT010000953         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3050527,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000953 DATLJT010000000
VERSION     DATLJT010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050527"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VM910_21840"
     CDS             <1..997
                     /locus_tag="VM910_21840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI65198.1"
                     /translation="DCRASAEADRAAFLFHLGDIVYNFGEARYYYDQFYEPFRNYPAP
                     IFAIPGNHDSFIVPGTNPENAPLVTFQRNFCSENPVITPEAGSLHRTAMTQPGVYFAL
                     DAPFVRIIGLFSNALEDPGVVSNESGHWPTASNVQIDFLRAQLTKIKHDKYAGAVLLA
                     THHPSFSYAPPTKDDGSGGNHGCSTAMMGEIDKVCHDVGVYPHAFLAGHAHNYQRYTR
                     IVEFGNREINVPFIVCGDGGHNVNTLVRAYQGQPAQEPLSGSDVLYLDAGAKTMKVKH
                     LILAKYDDRNYGYLRIQVDKDYLKIGFHQVGIRSLAQSRYDMVTVRLADHHMVAN"
     gene            1116..1295
                     /locus_tag="VM910_21845"
     CDS             1116..1295
                     /locus_tag="VM910_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65199.1"
                     /translation="MSQPETRTTARSTCPECKGRLAVLRIIAGRAGSEYWTMRCTRCG
                     GIHLDIVKMAETADA"
     gene            1463..>3102
                     /locus_tag="VM910_21850"
     CDS             1463..>3102
                     /locus_tag="VM910_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HVI65200.1"
                     /translation="MAKNPDRSKKPNKTAKSKAHRPEVQPIGPALAELLNPAINRGES
                     GVGSSTGLQPPPDNSWDRRSGGEAAAHRARASTQGKKEDVARRDAEGMGFDEAPQANY
                     GTSALVPTLDPELARQLGLPTEEDDAEALARPPRSKTEALGVKATADALETLIRDGRP
                     EFRKEDGSMKVWTPHRPPRPEKSEGGVRFEIKSEYEPKGDQPQAIAELVEGIKRSDRT
                     QVLLGVTGSGKTYTMAKVIEATQRPALILAPNKTLAAQLYGEFKNFFPDNAVEYFVSY
                     YDYYQPEAYVPRTDTYIEKDSSINEQIDRMRHAATRALLERDDVIIVASVSCIYGIGS
                     VETYTAMTFALKKGERIDQRQLIADLVALQYKRTSADFARGTFRVRGDVIDIFPAHYE
                     DRAWRVNLFGDVVENIEEFDPLTGHKQDDLEFIKIYANSHYVTPRPTLVQAIKAIKIE
                     LKQRLDQLNAQGRLLEAQRLEQRTTFDLEMMEATGSCAGIENYSRYLTGRRPGEPPPT
                     LFEYVPDNALVFTDESHVTVPQIGGMFRGDFRRKATLA"
BASE COUNT          645 a         1041 c          901 g          515 t
ORIGIN      
        1 cgactgccgc gcgtcggcgg aagccgatcg cgccgctttc ctgtttcacc tcggcgacat
       61 cgtctacaat ttcggcgagg cgcgctatta ctacgaccag ttctacgaac ccttccgcaa
      121 ctatcccgca ccgatcttcg ctattcccgg caatcatgac tccttcatcg ttcccggcac
      181 caatccggag aacgcgccgc tcgtgacatt ccagcgcaat ttctgctcgg aaaatcccgt
      241 gataaccccg gaagcgggct cgctgcatcg caccgcgatg acgcaacccg gtgtgtactt
      301 cgcgctcgat gcgcccttcg tgcgaatcat cggcctgttc tcgaatgcgc tggaggatcc
      361 cggcgttgtc tcgaacgaga gtggccactg gccgaccgca agcaacgtgc agatcgattt
      421 cctgcgggcg caacttacga agatcaagca cgacaaatat gccggcgcgg tgctgctcgc
      481 gacccatcat ccttccttca gctatgcacc cccgacgaag gacgacggca gcggcggcaa
      541 tcatggctgc agcaccgcca tgatgggtga gatcgacaag gtctgccacg acgtcggcgt
      601 ctacccgcac gccttcctcg ccggtcacgc ccacaactac cagcgctata cgcgcatcgt
      661 ggaattcgga aaccgggaga tcaacgtgcc cttcatcgtc tgcggcgacg gcggccacaa
      721 cgtcaacacg ctggtgcggg cctatcaggg ccagccggcg caggagccgc tcagcggcag
      781 cgacgttctc tacctcgacg ccggagccaa aacgatgaag gtgaagcacc tgatcctcgc
      841 caaatatgac gaccgcaact acggctacct tcggatccag gtcgacaagg actatttgaa
      901 gatcggcttc catcaggtcg gcatacggtc gctcgcgcaa tcgcgctacg acatggtcac
      961 cgtcaggctt gccgaccacc acatggtcgc gaactgagca accggcggcg gcaggacact
     1021 tcgcaaaaag gctggtcatg tgaacggctt cacatcccac ttggcggatt cgtggtccgc
     1081 taccaacagc ctgatcaaag agagtgatgc cagccatgtc acagccggag acccgaacga
     1141 ccgcgcgatc gacctgcccg gagtgcaaag gccggctggc ggtgctgcgc atcatcgccg
     1201 gacgcgcggg ttccgaatac tggaccatgc gctgcacccg ctgcggcggc attcacctcg
     1261 acattgtcaa gatggccgaa accgccgacg cttgatgtgt ccgcagatgc ggctgacacc
     1321 tgctgacatc tgaacgcaat tatcggcgtt ttcagaagca tactcacgcg cgtttcttcc
     1381 tttcgtccgg tgcgagccga gctgcggcct tgactgtttc ttggctcttt catgtcagcc
     1441 ggactcggcc catattccgg tcatggcgaa gaatcccgac cgatcgaaaa agcccaacaa
     1501 aacagcgaaa tccaaagccc accgccccga ggtccagccg atcgggccgg cgcttgccga
     1561 attgctcaat cccgcgatca accgcggcga aagcggcgtc ggttcctcga ccggcttgca
     1621 gccgccgccg gacaattcgt gggaccgccg ctcgggcggc gaggcagccg cgcatcgcgc
     1681 ccgcgcctcg acccagggca agaaggaaga tgtcgccagg cgggatgccg aaggcatggg
     1741 cttcgacgaa gccccgcagg ccaattacgg cacatccgcg ctcgtaccga cgctcgatcc
     1801 ggaactggcg cgacagctcg gtctgccgac ggaggaagac gatgcggaag cgctcgcccg
     1861 tccgccgcgc agcaagaccg aagcgctcgg cgtcaaggca accgccgacg cactggagac
     1921 gctgatccgc gacggccgcc cggaattccg caaggaagac ggctccatga aagtgtggac
     1981 gccgcaccgc ccgccgcgcc cggagaagag cgaaggcggc gtccgcttcg agatcaagtc
     2041 ggagtatgag ccgaagggcg accagccgca ggcgatcgcc gaactggtcg aaggcatcaa
     2101 gcgcagcgac cgcacgcagg ttctgctcgg cgtcaccgga agcggcaaga cctacaccat
     2161 ggccaaggtg atcgaggcga cgcagcgccc tgccctgatc ctcgcgccga acaagacgct
     2221 ggccgcgcag ctctatggcg agttcaagaa tttctttccc gacaacgcgg tcgagtactt
     2281 cgtctcctat tacgactact accagcccga agcctatgtg ccgcgcaccg acacctatat
     2341 cgagaaggat tcctcgatca acgagcagat cgaccgcatg cgtcatgctg cgacacgcgc
     2401 gctgctcgag cgcgacgacg tgatcatcgt cgcctccgtc tcctgcatct acggtatcgg
     2461 ttcggtcgag acctatactg cgatgacctt cgcgctgaag aagggcgagc gcatcgacca
     2521 aaggcaattg atcgccgacc tcgtcgcgct gcaatacaag cgcacctcgg ccgatttcgc
     2581 ccgcggcacc ttccgcgtgc gcggcgacgt catcgacatc ttcccggcgc actacgagga
     2641 tcgcgcctgg cgcgtgaacc tgttcggcga tgtcgtggag aacatcgaag aattcgatcc
     2701 gctcaccggc cacaagcagg acgatctcga attcatcaag atctacgcca actcgcatta
     2761 tgtaacgccg cgcccgacgc tggtgcaggc gatcaaggcg atcaagatcg agctgaaaca
     2821 gcggctcgac cagctcaacg cgcagggacg ccttttggaa gcgcagcggc tcgagcagcg
     2881 cacgaccttc gatctcgaaa tgatggaagc gaccgggagc tgcgccggca tcgagaacta
     2941 ttcgcgctat ctgaccggcc gccgcccggg cgaaccgccg ccgacgctgt tcgaatatgt
     3001 gcccgacaac gcgctggtgt ttaccgacga aagccatgtc acggttccgc agatcggcgg
     3061 catgttccgc ggcgatttcc ggcgcaaggc gacgctcgcc ga
//
LOCUS       DATLJT010000954         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2130771,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000954 DATLJT010000000
VERSION     DATLJT010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130771"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VM910_21855"
     CDS             complement(<1..496)
                     /locus_tag="VM910_21855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65201.1"
                     /translation="MSALVVLKREVRASARLWLVVAVAFFTAFQLLQLALLIVRFQAF
                     PNYLTLHDWPANIARIARMTPSVADMIPIMFDEWLVEVGSINYSFGRGIAEWSFVLIP
                     GKAVIALGIAALVATNFVLLRAAWKTCGILARLGSPVATGAGALVASAAATTITWVVC
                     CAAPT"
     gene            complement(493..1389)
                     /locus_tag="VM910_21860"
     CDS             complement(493..1389)
                     /locus_tag="VM910_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI65202.1"
                     /translation="MAAVDESADTAVTASPTTAEAESSRSSRPDAGDERSEWMAGLTM
                     SIARWAVRLSALLAGLVCWHYASSKGLTLYVRFEQIPGPLKVGEALINQLQHVAFYLH
                     IGASLQRIAISYSLAAGIGITLGLLMGRSRFIRDLIFPYVEIVRPIPGVAWIPLAILL
                     WPTEEGSIIYITFLGALFPIILNTLHGVEQTPEVLVRAAQSLGASRMCIFRHVVLPGA
                     LPSICAGLAIAMGVSWFSLLAGEIISGQYGIGYFTWNAYSIINPPDIIVGMLTIGLLG
                     AATTALVHLITRPALAWQRKAR"
     gene            complement(1459..2877)
                     /locus_tag="VM910_21865"
     CDS             complement(1459..2877)
                     /locus_tag="VM910_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65203.1"
                     /translation="MRQASFATVVAIRVPALMNLKCIPLLAMALLLPTASPAAAETID
                     IAIGHQTMCTDTYTGGIVIKELKLLEKHLPHTGKYTEATYNISWADYPSGGPITNQML
                     AGKLHFGTMGDYPLIVNGAKFQETKSLRTILFTGTGYNLKGAGNAIVVPVNSNSYKLE
                     DLKGKSISTPVGSASWGMLVKALQDKKMPLTSIDLKNQTPPVGASNIATNKVDAHSDF
                     CPWSEIMEFRGTGRKIYDGSEIGIPYLHGTVVRQDFAEKYPEVVVAFVKAVYDAGKWI
                     DEDPMRATDLMEKWTGVEKEVLYLYFSKGGHLTQDPTIKKQWVDTLKVDHQVLVNDNL
                     APALDFDAWIKEDYLKRAYAEMGVDYEAEKAKVVDTLVTNAGRPNEIWHARDGILSYP
                     TLKEFLKAVAEFRATGAKLNATYVYDQATGLKLFGKTAFFVHKRDGDFATFLRKGEAE
                     EFAAKIGGKVISFEEAVASSAS"
     gene            complement(3069..>3240)
                     /locus_tag="VM910_21870"
     CDS             complement(3069..>3240)
                     /locus_tag="VM910_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI65204.1"
                     /translation="IGAAFTHADLAHMVGATRQWVTISLQRLQEQGVVVCRKSQIIVR
                     RLDALSEMRGGG"
BASE COUNT          626 a          962 c         1015 g          637 t
ORIGIN      
        1 atgtgggcgc cgcgcagcat acgacccagg tgatggtggt cgccgccgcg ctggcaacga
       61 gtgccccggc gccggtcgca accggtgagc cgagccgggc aaggatcccg caggtcttcc
      121 aagcagcccg cagcagcacg aagttggtgg cgacgagtgc cgcaatcccg agcgcgatca
      181 cagccttgcc cggtatcagc acgaaactcc attcggcgat gccgcgcccg aacgaatagt
      241 ttatcgagcc gacttcgacg agccattcgt cgaacatgat cggaatcatg tcggcgaccg
      301 acggcgtcat gcgggcgatc ctggcgatgt tggcgggcca gtcgtgcaag gtcagataat
      361 tgggaaacgc ctggaagcgc acaatcaaga gcgcgagttg cagaagctgg aaggcggtga
      421 agaaggcgac cgccacaaca agccacagcc gggcgctggc gcgaacctcg cgcttgagga
      481 cgacgagggc gctcatctgg ccttcctctg ccaagcgagc gccggacgtg taatcaggtg
      541 caccagggcc gtggtggcgg cgcccaacag cccaatggtc agcataccga caatgatgtc
      601 cgggggattg atgatggaat aggcgttcca ggtgaaatag ccgatgccgt attgcccgct
      661 gatgatttcg cctgccagca acgagaacca agacacgccc atggcgatcg cgagacctgc
      721 gcagatgctc ggtagcgctc ccggcaggac gacgtggcgg aagatgcaca tgcgcgaggc
      781 accgagcgac tgcgccgcgc gaacgagcac ttccggcgtc tgctccacgc cgtgaagggt
      841 gttcagaatg atggggaaca acgcgccgag gaaggtgatg tagatgatcg agccttcctc
      901 ggtcggccag agcaggatgg ccagcggaat ccacgcgaca cccggaatcg gacgcacaat
      961 ctcgacatag gggaagatga ggtcgcggat gaaccgcgag cggcccatca gcagaccgag
     1021 cgtgatcccg atgccagccg ccagcgaata actgatggct atgcgctgca ggcttgcgcc
     1081 gatgtgcaag tagaacgcaa cgtgctgcag ttgattgatc agcgcttcgc cgaccttgag
     1141 cggaccggga atttgctcga accgaacgta taaggtgagc cccttggagc tcgcgtagtg
     1201 ccagcaaaca agtccggcca acagcgccga cagccgcacc gcccagcggg caatcgacat
     1261 cgtgaggccg gccatccatt ctgatcgctc atcgccggcg tcgggccgcg acgaacgcga
     1321 actttcagcc tcggcggtcg tcggcgacgc ggttaccgcg gtgtcggcgc tctcatcgac
     1381 ggccgccatt ttcactgtcg cttgcggggc aatgtgtgcg tgcgcgttca cgagtgagag
     1441 cgccttgctt tcggattgtc atgacgccga gcttgcgacg gcctcctcga acgagatgac
     1501 cttgccgccg atctttgcgg cgaactcctc ggcctcaccc ttgcgcagga aggtcgcgaa
     1561 gtcgccgtcg cgcttgtgga cgaagaacgc cgtcttgccg aagagcttga gtcccgttgc
     1621 ctggtcgtag acataggtcg cgttgagctt ggcgccggtc gcgcggaatt ccgcgaccgc
     1681 cttgaggaac tccttcagcg tcggataaga gagaatgccg tcgcgcgcgt gccagatttc
     1741 gttcggtcgg ccggcattcg tcacgagcgt atcgacgacc ttggcctttt cggcttcgta
     1801 atctactccc atctcggcgt aggcgcgctt gaggtagtcc tccttgatcc aggcgtcgaa
     1861 gtccaaggcg ggggcaaggt tgtcgtttac aaggacctga tgatcgacct tgagcgtgtc
     1921 gacccactgc ttcttgatcg tcgggtcctg cgtgagatgg ccgcccttgg agaaatagag
     1981 gtagagcacc tctttctcga cgccggtcca cttttccatc agatcggtcg cccgcatggg
     2041 atcctcatcg atccatttgc cggcgtcgta gaccgccttg acgaaggcga cgaccacctc
     2101 cggatatttc tcggcgaaat cttgtcgcac gacggtgccg tgcaaatagg gaatcccgat
     2161 ctcgctgccg tcatagatct ttcggccggt gccgcggaat tccataatct ctgaccatgg
     2221 gcagaagtcc gagtgggcgt cgaccttgtt ggtcgcgata ttgctggcgc ctacgggagg
     2281 cgtctgattt ttgagatcga tgcttgtgag cggcatcttc ttgtcctgca acgctttgac
     2341 cagcatgccc caggaggcgc tgccgaccgg cgtcgagatc gatttcccct tcaggtcttc
     2401 gagcttgtag gagttcgagt tcaccgggac gacaatcgcg ttgccggctc ctttcagatt
     2461 gtagccggtg cccgtgaaca ggatcgtgcg caggctcttg gtctcctgaa acttcgcgcc
     2521 attcacgatc agcggataat cacccatggt gccgaaatgg agcttgccgg cgagcatctg
     2581 attggtgatt ggaccgcccg acggatagtc ggcccaggag atgttgtagg tagcctcggt
     2641 gtacttgccg gtgtgcggca gatgtttttc gagcagcttg agttctttga tcacgatgcc
     2701 gccggtgtag gtatcggtac acatagtctg gtgcccgatg gcgatatcga tggtctcggc
     2761 cgcggcagga ctcgcagtcg gcagcaacaa cgccattgcc agaagaggga tgcatttgag
     2821 gttcattaaa gccggcaccc gaatcgcgac gaccgttgca aagctggctt gtctcatggc
     2881 ctctccctct ttctcgctgg cacgacgtgc gcggttcgac ggtctccccg gtttccggga
     2941 ttattggcct gcgtttgctg cgccgcaaac caggagtggc agtcacggtt attagttgcc
     3001 caaaatcacc cgctgcgcat attttcggca gttgcgcaga agaacgctca ttcactaggc
     3061 agcgagggtt acccgccgcc ccgcatctcc gagagggcat cgagacggcg cacgatgatt
     3121 tgcgatttcc ggcacaccac gacgccctgc tcctgcaatc gctgcaggct gatggtcacc
     3181 cattgccgag tcgcaccgac catgtgggca aggtcggcgt gcgtaaacgc ggcaccaatg
//
LOCUS       DATLJT010000955         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_981092,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000955 DATLJT010000000
VERSION     DATLJT010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981092"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VM910_21875"
     CDS             complement(<1..749)
                     /locus_tag="VM910_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI65205.1"
                     /translation="MEFLTTSEAADYLRLGERKLYELVTQGAIPCSKVTGKWLFPRHE
                     LDLWVMSGLARPSGMIVAEPPPIVGGSQDDLLEWSLRESGSALASLTEGTTRGVDRLM
                     RGEVIATAIHFHGSEVSADAAEDANLAAARALAGLHDAVLVGVVRREQGFLVAPGNPK
                     NLQSLADVLSVRARMAVRQPGAGAQLLLDALLARAGASPKDLHRREPPCLTGPDLANV
                     VRAGEADCGIATRAAAKSAGLDFVPLLWENF"
     gene            838..1530
                     /locus_tag="VM910_21880"
     CDS             838..1530
                     /locus_tag="VM910_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI65206.1"
                     /translation="MADQATAWQLILNGDAALFAIVRLSLLVSLSAVALAALIAMPLG
                     AILALTHFPARPVLVVLLNALMGLPSVVVGLAVYLLLSRSGPLGEMGILFTPSAMVIA
                     QTILIVPIIAALTRQTIEDLWLEYRDELTAMNVGPLGRMAALLWDARFSLMTALLAGF
                     GRAAAEVGAVMIVGGNIDGFTRTMTTAIALETSKGNLPLAMGLGLVLVVIVVAINAAL
                     WGTRVWADRKAG"
     gene            1533..2255
                     /locus_tag="VM910_21885"
     CDS             1533..2255
                     /locus_tag="VM910_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65207.1"
                     /translation="MRAPTSDLPLVLDDVSLDAGATRLIDRASLTIAPGAPTLIVGPN
                     GAGKTSLLRLCMGLAAPSSGRITWGGRIDRAPTRRAIVFQRPVMLRRSAAANLGFALG
                     QAGVPRRDRAKRIASLLDRVGLADLAQRPARRLSGGEQQRLALARALARDPELLLLDE
                     PTASLDPAATRSVEEIVLNAAQSGIKIVMTTHDLGQVRRLAGDVIFLLRGGVCERRPV
                     GDFLDNPAMPETVAFLRGDLVI"
     gene            2273..2850
                     /locus_tag="VM910_21890"
                     /pseudo
     CDS             2273..2850
                     /locus_tag="VM910_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(2878..3030)
                     /locus_tag="VM910_21895"
     CDS             complement(2878..3030)
                     /locus_tag="VM910_21895"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65208.1"
                     /translation="MMSGRSDLARNIPLGRLGRPEEVAQAVLMVLGNDYMTGQTIILN
                     GGMAFI"
     gene            complement(3042..3614)
                     /locus_tag="VM910_21900"
     CDS             complement(3042..3614)
                     /locus_tag="VM910_21900"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI65209.1"
                     /translation="MILTPDLKNRVAMVTCASRGIGAAIAVALAEAGAAVGLNYRERA
                     DAANALAADINAAKGRAVAIAADVSKAADVARMVEHATSQLGPIDILVNNAGIATVRG
                     VDDLSEEDFDRTIAVNLKSAFLCTQAVLPAMRKKKWGRIVNVSSGAARGAGAIGPHYN
                     ASKAAMEGLTRGYAARLVKEGITVMRSRRH"
     gene            complement(3631..>3710)
                     /locus_tag="VM910_21905"
     CDS             complement(3631..>3710)
                     /locus_tag="VM910_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HVI65210.1"
                     /translation="LDPFFNANTVEDIADAERLAALDGG"
BASE COUNT          581 a         1197 c         1228 g          704 t
ORIGIN      
        1 tcgaaattct cccaaagcag cgggacgaaa tcgagcccgg ccgatttggc tgctgcgcgc
       61 gtcgcgatgc cgcaatcggc ttcgccggcg cggacgacat ttgcaagatc gggcccggtg
      121 agacagggcg gctcgcggcg atgcagatcc tttgggctcg ccccggcgcg cgcgagcagc
      181 gcatccagca gcagttgtgc gcctgcgccg ggttgtcgca ccgccattcg cgcgcgcacg
      241 cttaggacgt ccgcaagact ctgcaggttc ttcggattgc ccggcgcgac caaaaatcct
      301 tgctcgcgtc tgactacccc gaccaaaacg gcatcatgca ggccggccaa tgcgcgcgcg
      361 gccgcgaggt tggcatcctc ggcggcatcg gcggagactt cactgccgtg gaaatggatc
      421 gcggttgcaa tgacctcgcc gcgcatcaga cggtcgactc cgcgcgtcgt gccttcagtg
      481 agagaagcaa gcgccgagcc ggattcgcgc aggctccatt ccagcaaatc gtcctggctg
      541 ccacctacga tcggcggcgg ctccgccacg atcatgccgg acgggcgcgc cagtcccgac
      601 atcacccaga ggtcgagctc gtgtcggggg aacagccact tgcccgtcac cttgctacag
      661 gggatcgcac cctgagtgac cagttcgtag agcttgcgtt cgccgagccg gagataatcg
      721 gcggcctcgc tggtggtcag aaattccatc cctgcatttg tatgcatatt tctgcgttta
      781 ttcaatcgtt tgacaattcc tggaatcttt gccgaacagg accggaggag cggtcggatg
      841 gcggaccaag caacggcctg gcaattgatc ctgaacggcg acgctgcact gtttgcaatc
      901 gtgcggctgt cgctcctggt cagcctgtcg gcggtggcgc tcgccgcgct cattgccatg
      961 ccgctcggcg cgattctcgc gttgacgcac tttccggcgc gtccggtgct ggtcgtgctt
     1021 ttgaatgcgc ttatgggatt gccgtcggtc gtcgtgggac ttgcggtcta tctgttgctg
     1081 tcgcgctccg ggccgcttgg cgagatggga atcctgttca cgccgagtgc gatggtgatc
     1141 gcccagacca tcctgatcgt tcccatcatc gccgcgctga cgcgccagac gatcgaggat
     1201 ctctggcttg aatatcgcga cgagctgacc gcgatgaatg tcggcccgct cggccgcatg
     1261 gcggcgctgc tttgggatgc gcggttcagc ctcatgaccg cgctgctcgc cggattcggc
     1321 cgcgccgcag ccgaggtcgg tgcggtgatg atcgttggcg gcaatatcga cggcttcacc
     1381 cgcaccatga cgaccgcgat cgcgctcgag acgtccaaag gcaatctgcc gctcgcgatg
     1441 ggactcggtc tggtcctggt cgtgatcgtg gtcgcgatca acgccgcgct gtggggcacg
     1501 cgcgtctggg ccgaccggaa ggcgggttga cgatgcgcgc gccaacaagc gatctgccgc
     1561 ttgtgctcga tgacgtctcg ctcgacgcgg gcgccacgcg gctgatcgat cgcgcgagcc
     1621 tcacgatcgc accgggcgcg cccacgctga tcgtcgggcc gaatggcgcc ggcaagacca
     1681 gcctgctgcg gctctgcatg ggactggcgg cgccgagctc cggccggatc acctggggcg
     1741 gccgcatcga tcgtgcgccg acgcgccgcg ccatcgtgtt ccagcgtccc gtgatgctgc
     1801 gccggagtgc cgcggccaat ctcggctttg cgttggggca ggcgggtgtg ccgcggcgcg
     1861 atcgcgcgaa acggatcgcg tcgctgctcg atcgcgttgg gctcgccgat ctcgctcaac
     1921 ggccggcccg tcggctctca ggcggcgagc agcagcggct cgcgctggcg cgcgcgcttg
     1981 cgcgcgatcc ggaattgctg ctgctcgatg aaccgaccgc cagtctcgat ccggccgcga
     2041 cgcgaagcgt cgaggagatc gtgctcaacg ccgctcagtc cggcatcaag atcgtgatga
     2101 cgacccacga tctcggtcag gtacggcggc ttgcggggga cgtgatcttt ctgcttcgcg
     2161 gcggcgtctg tgagcggcgg ccggtcggcg atttcctcga caatcccgcg atgccggaaa
     2221 ccgtcgcctt tctgcgcggc gatctcgtga tctgatcttc aaggaaacat cgatgtcatc
     2281 ccggttttgc gcgctcgctc ttgcggctct ttgttccgtc atcatcgctc gcgcgcccgc
     2341 cgctgccgag gagcgatcga tcgtcgtggc gtcgacgacc tcgacggaag attccggcct
     2401 gttcggctat ctcctgccga tcttcaagca gaagaccggc atcgacgtga aggtggtggc
     2461 gcagggcacg ggacaggcgc tcgacaccgc gcgccggggc gatgccgacg tcgtgttcgt
     2521 gcacgccagg gcgcaggagg agaagtttct tgccgagggc tttggcgtga agcgcttcga
     2581 cgtcatgttc gacgatttcg tgctgatcgg gccgaagagc gatcccgccg gtgtcagggg
     2641 caaggacatc gaaacagcgc tccgcacgat cgaaacgaag gctgcgccct tcgtgtcgcg
     2701 cggcgaccgc tccggcacgc actgaagcag gagctcggcc aggcgttcat cgactggctg
     2761 atctcggggg agggccaggc ggcgatcgcg agctacagga tcaacggcca gcaattgttc
     2821 tttccggatg ccgggaacgg accgtcttag ggcgggcctc gccgtcaggc aggctcttca
     2881 aataaaggcc atgccgccgt tgagaatgat ggtctggccg gtcatgtaat cgttgccgag
     2941 caccatcaac acggcctgcg ctacttcctc cggtcggccg aggcggccga gcggaatgtt
     3001 gcgggcaaga tcgcttcgcc cgctcatcat gtcggtttcg atcaatgacg gcgcgaccgc
     3061 atgacggtga tgccttcctt caccagccgc gcggcatagc cacgcgtcaa tccttccatg
     3121 gcagcctttg aggcattgta gtgcggcccg atcgcgcccg ctccgcgcgc ggcgccggag
     3181 gagacgttga cgatgcgtcc ccatttcttc ttacgcattg cgggcaggac ggcctgcgtg
     3241 cagagaaagg cggatttcag gtttacggcg attgtccggt cgaaatcctc ttcggagaga
     3301 tcgtcgaccc cgcgcacggt cgcaatgccc gcattgttga cgaggatgtc gatcggcccg
     3361 agctgtgagg tcgcatgctc gaccatgcgc gcgacatcgg ccgctttcga cacgtcggcg
     3421 gcaatcgcca ccgcccggcc tttggccgcg ttgatgtcgg ctgcgagcgc attggcggca
     3481 tcggcgcgct cgcgatagtt cagcccgacc gcggcgccgg cttcggccag tgcgacggca
     3541 atcgcggcac caatgccacg cgaggcgcag gtcaccatcg caaccctgtt cttcagatcg
     3601 ggtgtcagga tcatgtggcc gtccttttct ttaacccccg tccagtgccg caagccgctc
     3661 ggcgtccgcg atgtcctcga cggtgttggc gttgaagaac ggatcgagcg
//
LOCUS       DATLJT010000956        15403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1442310,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000956 DATLJT010000000
VERSION     DATLJT010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442310"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(574..1191)
                     /locus_tag="VM910_21910"
     CDS             complement(574..1191)
                     /locus_tag="VM910_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI65211.1"
                     /translation="MRRAVIYLRVSTLDQTTANQERELREIADRMGCEIVKVYKDHGI
                     SGAKGRDKRPAFDKLCRDAARREFDVVMAWSVDRLGRSLQDLVGFLSELHALRIDLFL
                     RQQGLDTTTPAGKAMFQMMGVFAEFERAMIAERVRAGLARARSEGKRLGRPSIAPALE
                     KRIREALALPGRPGVRVIAKQFGVNPGTVQRISRPFAAASIGGLT"
     gene            complement(1961..2284)
                     /locus_tag="VM910_21915"
     CDS             complement(1961..2284)
                     /locus_tag="VM910_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65212.1"
                     /translation="MGGGAKEDQEAPAAVTIVPGVWKEGLTKARYIATYRVALHILMR
                     NWEGGGQPFTLSNGAMALEGVGRGTKWRALRELEQLGLIRIERRKRKSPRITTLHTTA
                     NPEEA"
     gene            complement(2318..4180)
                     /locus_tag="VM910_21920"
     CDS             complement(2318..4180)
                     /locus_tag="VM910_21920"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI65213.1"
                     /translation="MLPLERWPYREIWLADFEFLAEAGERPAPVCLCARELRSGREVR
                     LWRDQLGPKPPYPTNADSLFVAYYASAELGCHLALGWPMPQRILDLFTEFRCGTNGLA
                     VPAGNGLIGALTAFGLDTIGTTEKDEMRALILRGGPWSDDERADILNYCAGDIDALAR
                     LLPAMAQRIDLPRALLRGRYMAAAASMEYAGTPIDAPMLDRLRRHWTAIQDRLIAQID
                     LDYGVFEGRTFKLDRFEAWLTRAGIPWPRLDSNRLDLSDDTFRQMARVYPAVAPLREL
                     RSSLAELRLNDLAVGRDGRNRTILSAFQARTGRNQPSNSKFIFGPSVWLRSLIKPPFG
                     MAIAYIDFEQQEFAIAAKLSADANMLAAYSSGDPYLAFAKQAGAIPPEGTKASHASVR
                     ELFKTTALAVLYGMEAEGLALKIDRPTIIARDLLRAHHETYRQFWRWSDAVVDHAKLT
                     GKLHTCFGWALQGCYDANPRSLRNFQMQAHGAEMLRLACCLATERGVEVCASVHDALL
                     IATPIDRIEAAITVTRMAMAEASRIVLGGFEVRTEAKVARSPNASPMRVAPACGASSP
                     NRLMKPGRERQACKWHEVTAKAAAAAERLRDLAEVERRLGLGRKWRERRDAQPL"
     gene            complement(4180..4818)
                     /locus_tag="VM910_21925"
     CDS             complement(4180..4818)
                     /locus_tag="VM910_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65214.1"
                     /translation="MTEKPRVVSPEDQPSEQAADDQPSEQAAPNPFDPERLRLDLSFS
                     EDEGVKKAIITIPVKKPSGQDFIRVHPSTAFRLPVAIIELRDDRETFLVLPAIARDIP
                     GEYNTVTMYTYINRQGVVFLWPVRLPGPDGRQLEWHRSAAEAAEMAMKRWVRVKANMS
                     LGAYEVYEAAATIPEPGWPPDLTFQQMLTIAFKGRLVDSFDHPVLKRLRGET"
     gene            complement(4836..5186)
                     /locus_tag="VM910_21930"
     CDS             complement(4836..5186)
                     /locus_tag="VM910_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65215.1"
                     /translation="MRTEDAFPGKYLKSSDAKPKPLVAVISHVEMEMVGQGQDQKRKP
                     VLHFEDQKPLVLNRTNFQALVDAFGDSDDWPGHKIKIYCVKTQYAGKSVDGLRIQAIA
                     PQPALADNNDEINL"
     gene            complement(5229..5549)
                     /locus_tag="VM910_21935"
     CDS             complement(5229..5549)
                     /locus_tag="VM910_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65216.1"
                     /translation="MSARIDMFGPAPAADNNLIGRKVKLDRPVDRERPCCNNICIISA
                     SKGPHTGALHCADCGQHRGWLSKTTAAWIESVITRFGAPTKPIVVRKSHTYEEEAPPT
                     EAQS"
     gene            complement(5546..5989)
                     /locus_tag="VM910_21940"
     CDS             complement(5546..5989)
                     /locus_tag="VM910_21940"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HVI65217.1"
                     /translation="MTVAPIIITIGGEPVAKGRPRATRRGMMYTPAKTRRYEAHGRLA
                     AQLAMDGQPPIGTPCRAEITIDLPVPQSWPQKRWDAALRGDTRPTTRPDADNYIKSAL
                     DSINEIAVTDDSLVVELVAVKRYAVPQLTITIAPLPAFTAQGRSA"
     gene            complement(5986..7248)
                     /locus_tag="VM910_21945"
     CDS             complement(5986..7248)
                     /locus_tag="VM910_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVI65218.1"
                     /translation="MSANGSAPPSVSATEGKGRGGNEAGRLLSPPNNAAADAVKFHPL
                     ADLFPLMEGAEFDALVADIKANGLQDSIVLYEGKILDGRNRYRACKAAGVVPRFRVGA
                     PSQEKPNLARPIIADPLAYVINANIHRRHLTAEQKRDLIAKLIKAQPEKSDRQIAETA
                     KASPTTVGTVRTEMEEVGDVSKLDTRTDKRGRKQPANKPKREPSEEREFEETIAPPVI
                     LEKLRAAELKITGLESEIEDLKAENADLRRKLEVCKSPVRCEFVEDDGGRAAAGYGGA
                     AGDCVARATAIATSKPYTEVFEALKARHAKYVKRHPDSWEADHMKRRRKAPIEYGCNE
                     KIYAPYLRSLGWQYTRIRERLCLRAGALPPGRLIVALDGHYVAVIDGVIHDTYDSGGA
                     GRRQMEGYWSQGDAEAGIELCRDREAQP"
     gene            complement(7245..7451)
                     /locus_tag="VM910_21950"
     CDS             complement(7245..7451)
                     /locus_tag="VM910_21950"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI65219.1"
                     /translation="MNQPEARVAYSIPEVMIQTGLGRDCVYRNIRARKLIARKIGRRT
                     VVLAADLHAFLNQLPKLHEGKSAA"
     gene            complement(7548..7949)
                     /locus_tag="VM910_21955"
     CDS             complement(7548..7949)
                     /locus_tag="VM910_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65220.1"
                     /translation="MALWRDRPKPKKQRRPKALSEKKMGELRPGQRHMGSLLRDIAEI
                     SVRYPTLTRGFTKQLRNLPKYENMDDRTLRRDVAEALASVESVLEKLWKRSPDLWPEL
                     LGIEPSPMLSKRALREKALEHLRRQLLAKTQ"
     gene            complement(8005..9342)
                     /locus_tag="VM910_21960"
     CDS             complement(8005..9342)
                     /locus_tag="VM910_21960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI65221.1"
                     /translation="MDISSCPCLDASADVRQCRPSNYGQLVWQGGRGDDSSTPPIVES
                     QGRAREAARHVCRRGGLYLRVAEGGSKQWIYRYTTNGHNRDMGLGPVRLLTLAEARER
                     ARAAGKLRLDGIDPIADKHAERGAAIVAAAGAKTFKECAEGFIRDNEKKWRHPKHRRD
                     WERTLVRFVFPTLGNLPVASIDTPLVLKVIKPLWERVPETASRVRGRIEAVLGWATVH
                     HYRAGDNPARWRQHLQHALPSRSEIAKVEHHAAMPYTQVASFMDALRKDTSIAARCLE
                     FITLTAARPGEATGATWDEIDLGARTWTIPAERMKKGDKEHKVPLSDAAMAVLNTMRE
                     IRQSAYLFPGFKPGQPIGADTLRELIKKLAGDDVTVHGLRSTFRDWAADCTSFPNELA
                     EMALSHAIPTAVEKAYRRGSMFEKRYQLMAAWANYCAGAATSEKVVPMPSARR"
     gene            complement(9472..10980)
                     /locus_tag="VM910_21965"
     CDS             complement(9472..10980)
                     /locus_tag="VM910_21965"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVI65222.1"
                     /translation="MSARPIRYLAAIALWTVPNHAGAQSPSTGTPEVLRVTRSADDGG
                     EGSLRWAIERNNAAPGRLRIELDPAGQPPHVIKLASPLPAIKGPVAIEGAPWKRSGEF
                     VVLDGSGYIEDKGPQTCPGAVAGQYGANVRTTSYPGLALVDTQGVEISGLEIRNFCIG
                     VLIHRASGNSIHDNRIVGNRGGAGVMLTGDDGNGNPTATTTVHNKVQRNQFLDNGDGL
                     ELTRGAAFNLVADNVFHSTDANPEPSQGIEILLGNDNVVVSNRFEGYSDGLQINWGHR
                     NYIAANIFTNNAFGLSVSGAGNSIDGNVISGNAVGIAVPPAADMTMASISHNAIYGNG
                     QKIERCFAGGSCDPNIRKGGIVFGLPSGEHERYVGKRGTGVAPAPGSLAKICPDGAPK
                     CQAPPNGGLAPPTLERVRKHAGELIVQGELRGKPRARFTVEVFGNRAAGSGEGEMFLG
                     EVAATSDAEGRGRFSLTIDAAKLAAMPASFTATLTSADGATSEFSQPVALAE"
     gene            11318..12412
                     /locus_tag="VM910_21970"
     CDS             11318..12412
                     /locus_tag="VM910_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI65223.1"
                     /translation="MKLQLTDWFCQHAKSSEPQTDYFDETVTGLALRVTSHGTKAWTF
                     LYGTPRRRITLGRYPSVSLATARALAIEAREGRTQVMTIAALTERYLQALKGKRSFRQ
                     IERLLRRDVLAIIGSIPLSELHRRDVTRVIDAKAAPIAARRAFDQLRAMIRWAVARGD
                     LDHNPIDGMKGPAASKPRERVLTDDEIRVLWLSLPQLRSPACQRVIKFCLVTGQRIGE
                     VTGLRRSELDLARKVWNIPGSRTKNGHPHSVPLSPMALEIIGESFDVVRRMYVSDTIR
                     ENQFGLPRWTTHDLRRTALTKMAELGVAPIVLGHVANHRTTTKAGITLGVYVQHAYEK
                     EKREALELWANRLQGIISGAGEVVAIGSGR"
     gene            12409..12948
                     /locus_tag="VM910_21975"
     CDS             12409..12948
                     /locus_tag="VM910_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65224.1"
                     /translation="MIDDDGWLNFHFAAKEIKAKLGVSLGKAQAMLRQLCASPGEVRS
                     RKQPYILVNQGTVNEEPAGQGPPELIEPSEWREHEIDLMTDDLGCWYFVDVRESDFRY
                     WLDQQKTKPELGKQPLIIRHLAEMFPNGIPDPAHCRRKALRADLLKRDPDLDPLDLKT
                     LKHAIDKYNLSFPIVGKQS"
     gene            13219..13584
                     /locus_tag="VM910_21980"
     CDS             13219..13584
                     /locus_tag="VM910_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65225.1"
                     /translation="MNDYGALSAADLAKQINDDYSMILNNERANLPRALAIAEKLNAL
                     RVRARRGEWKSKFESYGLKLSYETASVYLRIWEHWDDIKTLAVEKGVDPTMNLIMQGQ
                     GSRGLLNASLSVRLQGLQS"
     gene            14491..>15403
                     /locus_tag="VM910_21985"
     CDS             14491..>15403
                     /locus_tag="VM910_21985"
                     /inference="COORDINATES: protein motif:HMM:NF020289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65226.1"
                     /translation="MQAQQAGWLPEVEAAVATLIDADATKQQEACDWIEQKFGSEAAA
                     TAARLFAEARFFKNPEVQPREEGKDDFADFAGVEGGPHFRDERAKGDDDPPLLSKASP
                     FDSAKEFVRRHCFKEEMLATYYWEGDFWEWNDRCYEKVSAEKINAEIWAFLDRARSGT
                     HNDHRRFRPKPADVEGVMKALKAGLTLNVEAPCWLDGGPANSILVFKNGLVDIETGEL
                     MGLSPRLWVHHALDFEYDLEARCPVWERFLGEVFPGDQVSQDCIEEQLGYGMTDDNRF
                     HKGALWIGKGRNGKSTLAFIQEKLVGLS"
BASE COUNT         2994 a         4482 c         4760 g         3167 t
ORIGIN      
        1 agctgctcgg cgatttgcct atgcaggcct gcaggctcgt atctgggata cgtgtagcag
       61 gtgaagtcaa ttaagcttcg agccgacgct cggcgtgtcg cttcccgctg ggctgccttc
      121 tccagcccga gcagtgagtg accgtctaac gtcgacaagg agcgtgctaa gctcttcatc
      181 gctcatccca tcaaaagcgt ttgcgctctt gaaatgaagt tctctcggta ggattgacgc
      241 aattaccttc agataaattt ccggccgcgc ttcacgcacc ttctcgatga cagccggccc
      301 gtgcttcgcg aagtcgtcgt gtagcgcgag gatgaatttc tcattgagct tattgcggct
      361 gcctaggggg cggccctttg ggttgccgga gtggccgggc tgccactgcg gagccgatgg
      421 tttcggacgg gccattggat ttcctattct ggtgaacaga acgacgttct gtataacaga
      481 acaaagccgg aacggcaagg cggtcttacg gcccgagtga aatcgtgcga gttaccggga
      541 cgagttaggt cacgcgcctt tttcgcgcgg gggctatgtc aggccgccga tgcttgcggc
      601 ggcgaaaggg cggctgatgc gctgcaccgt gcccggattg accccaaact gtttggcaat
      661 aacgcgcaca ccgggccggc cagggagcgc cagggcctca cggattcgct tttcgagcgc
      721 aggggcaatc gagggccgtc cgagacgttt accttcactc cgagcacgcg caagcccggc
      781 acggactcgt tcagcgatca tcgcccgctc aaattcggca aaaacaccca tcatttgaaa
      841 catcgcctta cccgcgggcg ttgtggtgtc gaggccctgt tgacgcagga agaggtcgat
      901 cctgagcgcg tgcagctcgg agaggaagcc aactaaatcc tgcagactgc ggcccagccg
      961 atcgaccgac caggccataa ccacgtcaaa ttcccgccgt gcggcgtcac ggcacaactt
     1021 gtcgaaggcc ggacgcttgt cccgcccctt ggcaccgcta atcccatggt ccttgtagac
     1081 cttgacgatc tcacaaccca tgcggtcagc gatctcgcgc aattcacgct cctgattggc
     1141 cgtggtctga tccagggtgg agacccgaag gtagataacc gctcgacgca tggctcaatc
     1201 cctcccattt gggaagagca ctaatcaagc atccaaaatg acgcgttttg ttgggatttc
     1261 ggcaatgcaa tttcgaaggg ttttttcgta caccgtggtg tggctcgttg ctacgctcat
     1321 ccttcagcag ccatagcatc ggccgccact cgacggcagg cgctggagtt gcggcacccc
     1381 cctcgactgc cttcggtcgt tcaattcgtc tggaagcgag catgcgcttc taaaagagct
     1441 ttggacaccg gcgcaagggc ctcgcctcta ttctggacga agtcgtccag ccgcatctcg
     1501 gacaacttcc aaagcgagaa gctctcgaat ttgtagggac aggaagcgtc catccgatac
     1561 ggggcgacga gtcacgctcg gcttcgatag cccggccgca ggggcaatcc cgacgtcgga
     1621 cacgtgcggc cgaattcgag cgcgacgcac gggggaaggc cgtaagcgcg cgaaggaacg
     1681 cggcgtgcgc tttggttcgt ccgcgtaagc tataggacct ataacgtaga tccgacgaca
     1741 aatcggccgc ttggaatgat cgctatcggt acaagggcgg cgtgagtgcg aacgtccagc
     1801 cccgaagatc cagacccgca tttccctctg ccaatgacac gcacccaagg ggtacccacg
     1861 atatatattt aagacgaaga catatagact agagaccgta tgcacgccgg tgtctcatga
     1921 tatgagacct acggatgcaa ggcggttgca catctgcgtc tcatgcttcc tcagggttgg
     1981 cggttgtgtg taaggtggtg atccgcggcg acttgcgctt gcgccgctcg atcctgataa
     2041 gcccaagctg ttccagttcc cgcagcgccc gccatttggt gccgcgcccc acaccctcca
     2101 gcgccatcgc cccgttggat aacgtaaagg gctggccgcc gccttcccag ttccgcatga
     2161 gaatgtgaag cgcaacccgg taagtggcga tatatcgagc cttggtgagc ccttccttcc
     2221 atactcccgg cacgatggtg actgccgccg gcgcctcttg atcttctttg gcgccaccgc
     2281 ccacatctcg ccagtgtcaa tccgaacctt atctagatca aagcggttgt gcatctcgtc
     2341 gctcacgcca cttccttccc aatccaagac gacgctcgac ttcggcgaga tcccgcaagc
     2401 gctcggctgc ggcggcggcc ttcgcggtga cttcatgcca cttgcaggcc tgcctctccc
     2461 ttcccggctt catcaagcga ttgggtgacg acgctccaca tgcgggtgcc acgcgcatcg
     2521 gtgaagcgtt cggggagcgc gccactttgg cctccgtgcg cacctcgaac ccgccgagca
     2581 cgatgcgcga cgcctcagcc atggccatcc gcgtgactgt aatggcggcc tcgatccgat
     2641 cgattggagt cgcgatcagc aacgcgtcgt gcaccgaggc gcaaacttcg acgccgcgct
     2701 cggtggccaa gcaacaagcg agtcgcagca tttccgcgcc gtgcgcctgc atctggaaat
     2761 tccgcagcga gcgcggattg gcgtcgtagc aaccctgcag cgcccaccca aaacaagtgt
     2821 gcagcttgcc agtgagcttc gcatgatcaa caacagcgtc agaccagcgc cagaactggc
     2881 gataagtctc gtggtgagcc cgtagaagat cacgcgcgat gatcgtcggc ctatcgatct
     2941 tcagcgccaa accttccgct tccatgccgt agagcacggc aagcgctgtc gtcttgaaca
     3001 gctcgcgcac gcttgcgtgc gacgccttcg tcccttcggg aggaatggcg ccggcctgct
     3061 tggcgaaagc caaatagggg tcaccgctcg agtacgccgc caacatattg gcgtcggcgg
     3121 acagcttggc cgcgatggca aattcttgtt gctcgaagtc gatgtaggcg atcgccatcc
     3181 cgaaaggcgg cttgatcagc gatcgaagcc acacgctcgg gccgaaaatg aacttgctgt
     3241 tgctcggctg gttgcggccg gtgcgggcct ggaatgctga caaaatggtg cggttgcggc
     3301 cgtcgcgtcc aacggcaagg tcgttgagtc tcagctcggc gagcgagctt cgcagctcac
     3361 gcagcggcgc cacggccggg taaacgcgtg ccatttgccg gaatgtatca tcgctcagat
     3421 cgagccggtt gctgtcgagc cgcggccacg ggatgccggc acgcgtgagc catgcctcaa
     3481 agcggtcaag cttgaaggtg cgtccctcga aaacgccata gtccaaatcg atctgcgcaa
     3541 ttagcctgtc ctggatcgcc gtccaatgtc gtcgcagccg gtcgagcatg ggcgcatcga
     3601 tcggcgtgcc cgcatattcc atgctggcgg cggcggccat gtagcggcca cgcaagagtg
     3661 cccgcggcag gtctatccgc tgtgccatgg ccggcagtaa gcgagcgagc gcgtcgatgt
     3721 caccggcgca gtagttgaga atgtctgctc tctcatcgtc ggaccacgga ccgccacgca
     3781 ggatcagcgc gcgcatttca tccttctcgg tcgtaccgat ggtgtccaag ccgaaggcgg
     3841 taagcgcgcc gatcaaacca ttgccagcgg gaacggcgag cccgttggtg ccgcaccgaa
     3901 actcagtgaa gaggtcgagg atgcgttgtg gcatcggcca ccccagagcc agatggcagc
     3961 cgagctcggc cgaggcgtag taggcgacga aaagactgtc cgcgttggtc ggatacggcg
     4021 gcttcggtcc cagctggtcg cgccatagcc ggacttcgcg gccgctgcga agctcacgag
     4081 cgcataagca aacgggggcg ggacgctcgc ccgcctccgc taggaattcg aagtcagcta
     4141 gccagatttc tcgatatggc cagcgctcca gcggcaacat cacgtttctc cgcgcagccg
     4201 cttgagcaca gggtggtcga agctatcgac caaccggccc ttgaaggcga tcgtcaacat
     4261 ctgttggaag gtgagatcgg ggggccaccc aggctctgga atggtggcgg cggcttcata
     4321 cacttcgtag gcaccaagcg acatattggc tttgacgcgc acccaacgtt tcatcgccat
     4381 ctcggccgcc tcggccgccg agcggtgcca ttccagttga cggccatcag ggcccggcaa
     4441 tcgcaccggc cacagaaaga cgacgccttg ccggttgatg taggtgtaca tcgtcaccgt
     4501 gttgtattct ccggggatat cgcgagcgat agccggcaac acgagaaacg tctcgcgatc
     4561 gtcccgcaat tcgatgatgg cgacggggag cctgaaggcc gtgctcgggt ggaccctaat
     4621 gaagtcctgg ccgctgggct tcttcaccgg gatggtgatg atcgctttct tgacgccttc
     4681 gtcttccgag aagctgagat cgaggcgcaa ccgctcggga tcgaaggggt tgggtgcggc
     4741 ttgctcgctc ggttgatcgt ctgcagcttg ctcgctcggt tgatcctccg gcgagactac
     4801 gcggggcttc tcagtcataa cgtggtctcc tgtatctaga ggttgatctc gtcgttgttg
     4861 tcagcgagcg ccggctgggg ggcaatcgcc tggatcctga gcccgtcgac ggacttgccc
     4921 gcgtactgtg tcttgacgca gtagattttg atcttgtgac cgggccagtc gtcgctgtcg
     4981 ccgaacgcgt cgacgagcgc ctggaagttg gtgcggttga ggaccaaagg cttctggtcc
     5041 tcgaagtgca ggaccggctt gcgcttttgg tcctgaccct gtccgaccat ctccatctcg
     5101 acgtgcgaga tcacggcgac gagcggcttg ggtttggcgt cggaggactt gaggtatttg
     5161 ccggggaacg cgtcttctgt gcgcatggga aaagcctttc gctgttggcc acgcatgtgg
     5221 agttggtgtt aggattgagc ttccgtcggc ggcgcctcct cttcgtaggt gtgggacttg
     5281 cgaacgacga tcggtttcgt gggcgcgccg aagcgcgtga tgacgctctc gatccatgcg
     5341 gcggttgtct tggagagcca gccccggtgt tggccgcagt cggcgcagtg aagcgcacca
     5401 gtgtgcggcc ctttactggc gctgatgatg cagatgttgt tgcagcatgg ccgctcacga
     5461 tcgaccgggc gatcgagctt gaccttgcgg ccgatgaggt tgttgtcggc agcgggtgcg
     5521 gggccaaaca tgtcgatccg cgcactcatg ccgaccttcc ttgtgcggtg aaggccggga
     5581 gcggggcgat cgtgatggtg agctgcggca ctgcgtatcg cttgacggcc acgagttcga
     5641 ccacgaggga gtcgtccgtg acggcgatct cgttgatact gtcgagcgcg ctcttgatgt
     5701 agttgtcggc gtccggcctg gtcgtcggcc tggtgtcgcc acgcagcgcc gcgtcccagc
     5761 gcttctgcgg ccagctctgg ggcaccggca gatcgatcgt gatctccgcg cggcacggcg
     5821 tgccgatcgg cggttggccg tccatcgcga gctgcgcggc gagacgaccg tgggcctcgt
     5881 accggcgcgt cttcgcgggc gtgtacatca tgccccggcg cgtggcgcgc gggcggccct
     5941 tcgcgaccgg ctcgccgccg atggtgatga tgatcggagc gacggtcacg gctgcgcctc
     6001 tcggtcgcgg cacagctcga tcccagcctc ggcgtcgccc tgtgaccagt agccttccat
     6061 ctgcctccgg ccggccccgc cgctgtcata ggtgtcgtgg ataacgccgt cgatcacagc
     6121 aacgtaatgg ccgtcaagtg ccacgatcag ccgccccggt gggagggcgc cggcccggag
     6181 acacaggcgc tcccttatcc gcgtgtattg ccatcccaga gatcgcagat agggggcgta
     6241 gattttttca ttgcatccat actcaatagg cgccttgcga cggcgcttca tatgatcggc
     6301 ttcccaacta tcgggatggc gcttcacata cttggcgtgc cgcgccttca gcgcttcgaa
     6361 tacttcggta tatggcttgc tggtggcgat ggcggtggcg cgggcaacgc aatcccctgc
     6421 ggccccccca tatccggcgg cggctcggcc accatcgtcc tccacgaact cgcaacgaac
     6481 tggcgacttg cacacttcaa gcttccgccg caggtccgcg ttctcggcct tgaggtcttc
     6541 gatctcgctt tcgagcccgg tgatctttag ctcggctgcg cgtagctttt cgagaatgac
     6601 cggcggcgca atcgtctcct cgaactcgcg ttcttctgac ggttctctct tcggcttatt
     6661 cgccggctgc ttgcgcccac gcttgtcggt cctggtgtcc aacttggaca cgtcgccgac
     6721 ctcctccatc tcggtgcgca ccgtcccgac cgtcgtcggg ctcgccttcg ccgtctcggc
     6781 aatctgccgg tcggattttt cgggctgcgc ctttatcagc ttcgcaatca ggtcgcgctt
     6841 ctgttcggca gtcaggtgac ggcgatggat attggcgttg atgacgtagg ccagcgggtc
     6901 cgcgattatc ggtcttgcga ggttaggctt ctcttgcgat ggagcgccga cgcgaaatct
     6961 cggaacgacg cccgccgcct tgcaggcgcg gtatcggttg cgcccgtcga gaatcttgcc
     7021 ttcataaaga acaatcgagt cttgcagtcc gttcgccttg atgtcggcaa cgagtgcatc
     7081 gaactccgcg ccctccatca acgggaagag gtccgcgagc ggatgaaact tgacagcgtc
     7141 ggcggctgcg ttatttggag gggagagaag ccgtccagct tcgtttccac ctcggccctt
     7201 gccttcggtc gccgaaaccg aaggcggggc cgaaccgttt gcgctcatgc cgcgctcttg
     7261 ccctcatgca gcttgggcag ctggttcaag aaggcatgca agtccgcggc gagcacgacg
     7321 gttctcctgc cgatcttcct tgcgatcagc ttacgggcgc ggatgttgcg gtagacacaa
     7381 tccctgccaa ggccagtttg aatcatgacc tcggggatcg agtacgcgac gcgggcttca
     7441 ggttggttca cgttgatcgt cctctttaaa gccgtcaggg tgccggcaga ggactactga
     7501 cttatttgac agcaaagggc gctgtccaac acgttatcgg acaacaatta ctgcgttttg
     7561 gccaacaatt gcctgcgcag gtgctcaagt gctttttcgc gcaaggcgcg cttcgataac
     7621 attggtgatg gctcgatacc aagcagctcc ggccaaagat caggtgaacg tttccaaagc
     7681 ttctccagaa cgctctctac cgacgcgagc gcctcggcaa cgtcgcgcct cagcgtccgg
     7741 tcgtccatgt tctcgtactt gggcaagttc cgcagttgtt tggtaaaacc tcttgtgaga
     7801 gttggatacc gaacgctgat ctccgcaata tccctgagca ggctccccat gtgccgttgg
     7861 cccggcctta gctctcccat ctttttctcc gacagcgcct tcgggcgacg ttgcttcttc
     7921 ggctttggcc gatcgcgcca gagcgccatc tcaagcagag ggccagcgta gtcggggcca
     7981 tcgctttttc ccgacatagc gccatcaccg ccgcgccgaa ggcatcggca cgaccttctc
     8041 gctggtggca gcgccagcgc agtagttggc ccacgcagcc atgagctggt agcgcttctc
     8101 aaacatgctg ccgcgccggt acgccttctc aactgcggtc ggaatcgcgt gcgagagcgc
     8161 catctcagca agctcgttcg ggaacgacgt gcaatccgcc gcccagtctc ggaacgtgct
     8221 gcgcaagcca tgaacggtga catcgtcgcc cgcgagcttc ttgatcagct cgcgcagggt
     8281 gtcggcgccg atcggttgcc cgggcttgaa gccggggaag aggtaagcgc tttgtcggat
     8341 ttcgcgcatc gtattgagca ccgccatggc ggcatccgac agcggcacct tgtgctcctt
     8401 atcgcccttc ttcatacgct cggcggggat cgtccaggta cgcgcgccga gatcgatctc
     8461 gtcccaggtt gcgccggtcg cctcgccagg gcgggcggcg gtgagcgtaa tgaactcaag
     8521 gcatcgcgcc gcgatgctcg tatccttgcg tagcgcgtcc atgaagctcg ccacctgggt
     8581 gtagggcatg gcggcgtggt gttcgacctt ggcgatctcg gaacgtgatg gcagcgcgtg
     8641 ctggagatgc tgtcgccagc gtgccgggtt gtcacccgcc cgataatgat gaaccgtagc
     8701 ccatccgagc acggcctcaa tcctgccgcg cacgcgcgag gccgtctccg gcacacgctc
     8761 ccacagcggc ttgatcacct tgagtaccag cggcgtgtcg atcgaggcga cgggcaggtt
     8821 gcccaatgtc ggaaacacga accggaccag cgtccgttcc cagtcgcgcc ggtgcttcgg
     8881 gtgtctccat ttcttttcgt tgtcgcgaat gaagccctcg gcgcactctt tgaatgtctt
     8941 tgcgccagca gctgcgacga tggcggcgcc gcgctccgca tgcttgtcgg cgatcggatc
     9001 gatgccatcg agccggagct tgcccgccgc gcgcgcacgc tcacgagctt ccgccaacgt
     9061 gagcaggcga actggaccga ggcccatgtc gcggttgtgg ccgttagtgg tgtatcgata
     9121 aatccactgt ttcgagcccc cttcggcgac gcgaaggtag aggccgccgc gtcggcatac
     9181 atgcctggct gcttcgcgcg ctcgaccttg agattcgaca atcggtggag tgctcgaatc
     9241 atctccacgg cctccctgcc atacgagctg cccatagttg gacggtcgac actgacggac
     9301 gtcggcagac gcgtccagac aggggcagct cgagatgtcc agatatcacc gacatattca
     9361 cgatgctggc agacgtggcc ggacggccac gctatcggcg ggcggggtta aagcgtctcc
     9421 acggtctctt cggtgaggca gacgatgcgc tcgggcggaa acatccgccg ctcactccgc
     9481 cagcgcaacg ggctggctga attccgaggt ggcgccgtcc gccgacgtga gcgtggccgt
     9541 gaatgaggcc ggcatcgcgg cgagcttggc ggcgtcgatc gtgagcgaaa atctgccccg
     9601 cccctcggcg tcggacgtgg ccgcgacctc gccgagaaac atttcgcctt cgccgctgcc
     9661 cgccgcccga ttgccgaaga cttccacggt gaagcgcgcg cgcggcttgc cgcgcagctc
     9721 gccttgcacg atgagttcgc ccgcgtgctt gcgcacgcgc tcgagcgtgg ggggagcgag
     9781 gccgccgttc ggcggcgctt ggcacttggg cgcgccatcc gggcaaatct tggcgaggct
     9841 tcccggcgcc ggagcaacgc cggtgccgcg cttgccgacg tagcgttcgt gctcgccgct
     9901 cggtaggccg aacacgatgc cgcccttgcg gatgttgggg tcgcaactgc cgccggcaaa
     9961 gcagcgctcg atcttttgac cgttgccgta gatggcgttg tgggaaatcg acgccattgt
    10021 catgtcggct gccggcggca ctgcgatgcc cacggcgttg ccggagatga cgttgccgtc
    10081 gatgctattg ccggcgccgg agacgctcag gccgaacgca ttgttggtga agatgttggc
    10141 ggcgatgtag ttgcggtgcc cccagttgat ttgcaggccg tcggaatacc cttcgaaacg
    10201 gttgctcacg acgacgttgt cgttgccgag caggatttcg atgccctgtg acggttccgg
    10261 gttggcgtcg gtcgagtgga agacattgtc ggccaccagg ttgaaggccg cgccgcgcgt
    10321 gagctcgaga ccgtcgccgt tgtcgaggaa ttggttgcgc tgcaccttgt tgtggaccgt
    10381 ggtggtggcg gtgggattgc cattgccgtc gtcgcccgtg agcatcacgc cggcgccgcc
    10441 gcgattgccg acgatgcggt tgtcgtgaat cgaattgccg ctcgcgcggt ggatcagcac
    10501 gccgatgcag aagttgcgaa tctccaagcc gctgatctcg acgccctggg tgtcgaccaa
    10561 agcgaggccg ggatagctgg tcgtgcgcac gttggcgccg tattggccgg cgacggcgcc
    10621 ggggcaggtt tgcgggccct tgtcctcgat gtagccggag ccgtcgagga cgacgaattc
    10681 gccgctacgc ttccacggcg cgccctcgat cgccaccggg cctttgatcg ccggcagcgg
    10741 cgaagcgagc ttgatcacgt gcggcggctg acccgccgga tcgagctcga tgcgcaagcg
    10801 tccgggcgcc gcgttgttgc gctcgatcgc ccagcgcagc gagccttcgc cgccgtcatc
    10861 ggcgctgcgc gtgacgcgca gcacctcggg cgtcccggtc gaagggcttt gcgcgccggc
    10921 atgatttgga accgtccaaa gcgcaatcgc cgcgaggtaa cgaatgggac gtgccgacac
    10981 attctcctcc caaagttcgt tgcggcggtg gcgccgcatt cgccggatgg cccgttcccg
    11041 cgagcgtgca cctctcggac cacgtcggca aggcggcggg ccatattggc ttatgcgggt
    11101 catatcacca caccaacacg tccaggcaat tccctgctat tcgatgcaat cccctgtcac
    11161 ggcttgcaag gccaacttaa gctcattcca tcgggacaag ttgtcgcttt aagatgccgg
    11221 gacttgcgcc caaacggatg cacgtttacc tgccaggtac atcaaaggtg caggcttcgc
    11281 caagtcggcg ctgctcaacc tccgaggatc gcgacccatg aagctgcaac tcactgattg
    11341 gttctgccaa cacgcgaagt cctccgaacc tcaaacggat tacttcgatg agaccgtgac
    11401 gggcctagct cttcgagtaa cgagccacgg gacgaaggct tggaccttcc tttacggaac
    11461 accaagacgg cggatcactc tcggcagata tccctccgtc tccttggcga cggctcgtgc
    11521 tctggccatc gaggcgaggg agggccgaac acaagtgatg accatagccg ctttgactga
    11581 gcgttacctt caggccttga aaggcaagag gtccttccgt caaatcgagc gcctacttcg
    11641 cagagatgtt cttgcgatca tcggctctat cccgctaagt gaattacatc gccgtgacgt
    11701 tactcgggtt attgatgcca aggcagcccc aatcgcagca aggagagcct tcgaccagct
    11761 acgggcgatg atccgttggg ctgtcgctcg tggcgacctt gaccacaacc ccatcgacgg
    11821 aatgaaaggg ccagcggcat cgaagccccg cgagcgggtg ctaaccgatg atgaaatccg
    11881 ggtcctgtgg ttgagcttac ctcaactccg ttcccccgct tgccaacggg taatcaagtt
    11941 ctgtctggtg acaggccagc gaattggcga agtcaccggc ctacggcgca gtgagttaga
    12001 ccttgcccgc aaggtttgga atataccagg ctctcgaacg aagaacggcc atccccacag
    12061 cgtcccgtta tcgccgatgg cccttgagat tattggagag agcttcgacg tagtccgtcg
    12121 tatgtacgtg tcggatacga tccgtgagaa ccagtttggt ctacctcgct ggaccaccca
    12181 tgacctcagg aggacggcct tgaccaagat ggcagaactc ggcgttgcac caatcgtcct
    12241 tggtcatgtt gccaatcatc gaacgacaac aaaagcagga ataactcttg gggtgtacgt
    12301 gcagcacgcc tacgagaaag agaagcgtga agcgcttgag ctttgggcga atcggttgca
    12361 gggaattatc tcaggtgcgg gtgaagtggt ggcaattggg agtgggcgat gatcgacgac
    12421 gatggttggc tcaacttcca tttcgccgca aaggagatca aggccaagct tggcgtaagt
    12481 cttggcaagg cccaagcgat gctcagacag ctctgtgcta gtcctggtga ggttcgatcc
    12541 aggaaacagc cttacatcct cgtcaatcag ggcaccgtca atgaggaacc cgcagggcaa
    12601 gggccaccag aattgatcga acctagtgag tggcgtgagc atgaaatcga cttgatgacc
    12661 gatgaccttg ggtgttggta tttcgttgat gttagagaga gcgactttag gtattggctc
    12721 gaccaacaga aaaccaagcc ggagttgggc aagcagccac taatcataag gcatctcgct
    12781 gaaatgttcc ccaacggaat ccccgatcct gcccattgcc gacgtaaggc gttaagagcc
    12841 gacttgctca agcgtgatcc cgatctcgat ccactggacc tgaagacact caaacatgcg
    12901 attgacaaat acaatttgtc gttccccatc gttgggaaac aatcgtaatc ccactgtttc
    12961 cgactgatcc gtcgctgacg ctggggcact gtgacgccac caactccaac gaggaggcct
    13021 cacagtggaa ccacagctat tggataaggc cgaggtctgt cgcctattcg gcggcaaccg
    13081 gccactcaac ccctcgacac tttatcgcgg aataaagcag ggtcgatttc ccaagccggt
    13141 aaaggtcggc cctggctcta gccgatggct cctatcggag tgtgaagcgg cgttgcgggc
    13201 gatgatggag ggcaggtcat gaacgactat ggcgctctgt ctgctgccga cctcgctaag
    13261 cagatcaacg atgattactc gatgatcttg aataacgaga gagccaacct cccacgagcc
    13321 ctcgctatag ccgagaagct caatgcttta agggttaggg ctaggcgagg agaatggaaa
    13381 agtaaattcg aatcctatgg cctcaagcta agctatgaaa cagcttcggt ataccttcgg
    13441 atatgggagc actgggatga cataaaaaca cttgccgtgg aaaaaggtgt tgaccctaca
    13501 atgaacttga ttatgcaagg gcaaggttca agaggtctgt tgaacgcctc tttgagcgtg
    13561 aggttgcaag gattgcaaag ctaattgagg agccgggtcg atactcggtc agggaatggg
    13621 aaaaggtaag aacttatttt gaatggatcg caagaaggtt cgcgaattta gtggagcaaa
    13681 tggagcaagc aaggccacta accacgattg atgacacgcc cgttaaagac gccgtactga
    13741 agcctggggg aggcaagccc aagctaacca agagtgaggt tagccaaatc aagaccctcg
    13801 ttaatgaacg caagcttagc ttggaggaaa ttgcagcgca attcaaggtt agtcctgccg
    13861 aggtatctgc aatcaagaac gagaggagct ggcccgaggt cgaacctcga attgtgggcc
    13921 ggttggatcg ctatgaggcc aacgccgggg ccaacgttgg tgaagtggac gaacgttcca
    13981 gttccatcga tagggattcg gcggaaacgc cccatccgcc aaaacatagc cgtgtcactc
    14041 ttgcagatgg cggtgatcgg gggcgtgctg ctccatcgaa tatacgaagg agattttaga
    14101 tgcagagttg acgctggagc acaacgcaga aggcgaagag aactcgatcc gtgtcggctt
    14161 ggctttttgg gctgactgac tgagataatg aatgatggag gtgagatgaa acccgaaaga
    14221 gaattaagcc cagaagagaa agctcaactc catcgcgagt tggaggagat attctttggg
    14281 catcgccgac gacggcctcg ggcagtgttg gtttgccttg gaggtcgagt tgtcggtgag
    14341 gccacggtgg tcgttggtcc gcttgatcca aattggaggg caggtggaga gataaaggtc
    14401 cgaagatgat atcccgaacg aagtgttgaa gtcaggcgaa gggctcccgc aacggagccc
    14461 ttttgatttg gagtggaatg gagcgaggtc atgcaagctc aacaagcggg atggctgcca
    14521 gaggtagagg cggcagttgc aacgttgatt gatgctgacg ccacaaagca acaggaggct
    14581 tgcgattgga ttgagcaaaa gtttggctcg gaagcggcgg cgacggcggc gaggttattt
    14641 gctgaagcta ggttttttaa aaaccctgag gtacaaccta gagaggaagg taaagatgat
    14701 ttcgccgatt tcgctggagt ggaagggggt ccacattttc gcgatgaacg agctaaagga
    14761 gatgatgatc ctccgttatt gtcgaaagcc tcaccctttg acagcgccaa agagtttgtg
    14821 aggcgacatt gcttcaaaga ggaaatgctt gccacttatt attgggaagg ggatttttgg
    14881 gaatggaatg accgttgcta tgaaaaggtg tcggccgaga aaatcaatgc agagatatgg
    14941 gcttttctgg atagggccag gtccgggacc cacaatgatc atcgtaggtt ccggccgaag
    15001 ccggccgatg tcgaaggagt aatgaaggca ctgaaagctg gccttacttt gaatgttgaa
    15061 gcgccttgct ggctagatgg agggcccgct aatagtattt tagtgttcaa gaatggcctg
    15121 gttgatatcg agaccggaga attgatgggg ctaagtccaa ggctatgggt acatcatgca
    15181 ctggactttg agtacgatct ggaagcgaga tgtccggttt gggagcgatt tcttggagag
    15241 gtgttccccg gcgatcaggt ttctcaagac tgtattgaag aacaactcgg ctatgggatg
    15301 accgatgata acaggttcca taaaggagca ttgtggattg gaaaagggcg caatggcaaa
    15361 agcacgttgg catttattca ggagaagttg gtgggcttaa gcg
//
LOCUS       DATLJT010000957         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_523899,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000957 DATLJT010000000
VERSION     DATLJT010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523899"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VM910_21990"
     CDS             <1..288
                     /locus_tag="VM910_21990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI65227.1"
                     /translation="FRAIGLDHPRVTVVTISPEARISLLATGHFLSIFPASALRFPIK
                     RSEIKVLPIALPVACVPNGIVTLKNRTLSPVARLFIQHAREVAKPLVKKKS"
     gene            868..1062
                     /locus_tag="VM910_21995"
     CDS             868..1062
                     /locus_tag="VM910_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65228.1"
                     /translation="MRKISSFAVAAAALVVTGFGVLATSTTNGRVNAAIGHGIEPFQL
                     MVNAKELPTVEFADYTFVFH"
     gene            1293..3212
                     /locus_tag="VM910_22000"
     CDS             1293..3212
                     /locus_tag="VM910_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI65229.1"
                     /translation="MTRQAHLVGSVGLRDVETVLRTVSESLRPGRPRNTHIDLEKKVE
                     VLTRELGKALEQQAAVSEILRVISSSPGNLQTVFETILANATRLCEAIYGALWLSEGD
                     RFRNAAFHGALPAEYMELWRSATVSRTAPVGRVAQSRKPVQIADLREDQTYLDGHPLT
                     ITAVHVAGIHTLAIVPMLKENEFVGAIAIYRKEVRPFTDKQIELVTTFADQAVIAIEN
                     TRLLNELRESLQQQTATADALKVISRSTFDLKSVLQTLVESAVRLCEADMAAIARQKG
                     TKYHLVARHGYPSGYSEHIETLPMEPGRGSITGRVLLEGKRVHIIDVVADPEYTLVEA
                     QKRAGYRTMLGVPLLRDGIPIGVLHVLRTVVRPFTDKQIELVETFADQAVIAIENVRL
                     FDEIQDKSRQLAEASERKSQFLASMSHELRTPLNALIGLTEMMVANAGRFGTEKALEP
                     LRRVNAAGTHLLSLINEVLDLSKIEAGKLDLNPETIDLTRLIDEVIGTAGQLAEKNKN
                     HLVVEAPENVGTLTADSMRLKQILLNLLSNACKFTKEGEVALRVRKVADGRDWVELAV
                     ADSGIGLTAEQQAKLFQDFTQADSLTARRYGGTGLGLALSRKLARMMGGDVTVTSEPG
                     KGSVFTVRLPGSADS"
BASE COUNT          776 a         1053 c         1049 g          680 t
ORIGIN      
        1 tttcgggcga ttgggctcga tcatccgcgc gtaacggtgg tcaccatttc cccggaggcg
       61 cggatcagcc tcctggcgac cggacacttt ctctcgattt tcccggcgtc agcattgaga
      121 tttcccatca aacgctcgga gatcaaggtg ttgcccatcg cacttcccgt ggcttgcgtg
      181 ccgaatggaa ttgttaccct caagaaccgc acgctcagcc cggtcgcgcg gcttttcatt
      241 cagcatgccc gcgaggttgc gaagccgctc gtgaagaaaa agtcataggc gccatgtcgg
      301 cacagaaaaa agtacggccg ttgctggaga gctcggcctc tacccgttga aaacacactg
      361 cggcgcgata ataagagtcg gggtttttga cctgtttttc ctgctcaccg cgcagcaaat
      421 ctgtggtgcg catgacgata acatctggat tcggaagttc aagaacggac tcatattcgt
      481 ctgcggtcag tcacattgac ctgtaaggaa aattgccgcc ccgtggcttt ggcaatattg
      541 cctcgcttgg cgtggttttg cgctcatcgc tgcactcgcc ccgttgcttc aagacgcgga
      601 gcgtcaacat aaactgcagt cggaaaagca ccggccctcg cccgcatcaa aggctgcaag
      661 cgtagctcgc gcccaccggc gatgtcattg ctcctcgaac tcgtccgacc gatcagcgaa
      721 tgtggcgaga atctcgacga gcacatggtc gcgacgaagg ccgaaagccg tagctttcga
      781 ccaaggtgta atagactaat tgtgcattgc aataatctgc gctgcggcgc accaaacagc
      841 taattgcggt aaacgtggag accccagatg cgcaagatca gttcgtttgc cgttgccgcg
      901 gccgccctgg tcgtgactgg cttcggagta ttggcgacct cgaccactaa tgggcgggtg
      961 aacgccgcga taggtcatgg gattgaaccg ttccagctaa tggtgaacgc aaaggaactg
     1021 cccactgtgg agtttgctga ctataccttc gtgttccatt gaccagcggc cccgtagaga
     1081 ccggcattat cttggaaccc gatcgagaca aagctgaagc tgccgcgaag gacgcgattg
     1141 agcccgcata ggtcgaacag cgccccgctc cgtcggccct ggccaacgaa gaaagcaaca
     1201 aagtgtgatg accatcacaa atccgaaccg ctggctgcgc cgagggcggc aaccccgata
     1261 acgacgtggg cgggaaatcc ggagaaacag tgatgacgcg gcaggctcat ctcgtcggaa
     1321 gcgttggttt gagggacgta gaaacggtgt tgaggaccgt ctccgagagc ctcaggcccg
     1381 gccgcccgcg caacacccac atcgacctgg aaaagaaagt cgaggtgctc acccgcgaat
     1441 tgggcaaggc gctggagcag caggcggcgg tatccgaaat tctgcgcgtc atttcgagct
     1501 cgccgggaaa tctgcagacc gtattcgaga caatcctggc gaacgcgacg cggctctgcg
     1561 aggccatcta cggcgctttg tggctcagcg aaggagatcg cttccgcaac gctgcattcc
     1621 acggtgcgct gccggcggaa tacatggagc tgtggcgcag cgccacggtg agccgcacag
     1681 cccctgtggg ccgcgttgct caatcccgaa agccggttca gatagctgac ctgcgagaag
     1741 atcagacgta tctcgacggc catccattga ctattaccgc agtccatgtc gccggcatcc
     1801 acacattggc tatcgtgccc atgctcaagg agaatgagtt cgtcggcgcc attgccatct
     1861 atcgtaagga ggtccgtccg ttcaccgaca agcagatcga gctggtcacc accttcgccg
     1921 accaagcggt catcgccatc gagaacaccc ggctactcaa cgagctgcgc gaatcgctgc
     1981 aacagcagac cgccaccgcc gatgccctca aggttatcag ccgctctacg ttcgatctta
     2041 agtcagtgct tcagacgctg gtcgaatcgg ccgtccgact ttgtgaggcg gatatggcgg
     2101 cgatcgctcg tcagaagggc acgaaatacc accttgtggc gaggcatggg tacccgtccg
     2161 gctacagtga acacatcgaa actttgccaa tggagccggg ccggggatca ataacgggcc
     2221 gagttctgct tgagggcaaa cgggttcata tcatcgatgt tgtagccgat ccggaataca
     2281 ctttggttga agctcagaag agagccggct atcgcacaat gctcggtgtg ccgctcttgc
     2341 gagacgggat ccctattggg gtgctccacg tgctccgcac agtcgtccgg ccgtttaccg
     2401 acaagcaaat cgagctggtc gaaaccttcg ccgaccaggc ggtgattgcc atcgagaacg
     2461 tacgactatt cgatgaaatc caggacaaga gccgccagct ggcggaggcg agcgagcgca
     2521 agtcccagtt tctcgccagc atgagccacg agctgcgcac gccgctcaat gccctcattg
     2581 gcttgacgga aatgatggtc gccaacgcgg ggcgcttcgg cacggaaaag gcgcttgaac
     2641 cgctgcgtcg cgtgaatgcc gccggaactc acctgttgag cctgatcaac gaggtcctag
     2701 acctctccaa gatcgaagcc ggcaagctcg acctcaatcc agagacaatc gatctgacgc
     2761 gcctcatcga cgaggtcatc ggcaccgcgg gacaacttgc cgagaagaac aagaaccacc
     2821 ttgttgtcga agcgccggag aacgtgggca cgctcaccgc ggactccatg cggctcaagc
     2881 agattctgct caatctctta agcaatgcct gcaagttcac gaaggagggc gaagttgcgc
     2941 tgcgcgtgcg caaggtggcg gacggacgcg actgggtcga gcttgcggtc gcggatagcg
     3001 gcatcggctt gaccgcggaa cagcaggcca aactgttcca ggacttcacc caggccgatt
     3061 cactgaccgc ccgccgctac ggcgggacgg ggctcggcct tgcgttatcg cgcaagctcg
     3121 cgcgcatgat gggtggcgac gtgactgtga cgagcgagcc gggcaagggc tcggtgttca
     3181 cggtgcgcct gcccggaagc gccgacagct aaccaaaggc tgcaaaaatg ctactgagac
     3241 cggtgctaat ttgctgcacc gcgttatgtc cgctcatggc tgcgggttca actggtcact
     3301 gcaacactgc tcgtagtcta tcggcagggg tttcgaagcc caaggtcttc cgccggcgcg
     3361 acagcagcat cgatcgtccg gccgcagcgg ataattcttt tctcatcaag tcgttgcatc
     3421 tagagcagat tcagctcgat tttacgcata gcctgcatgc ctgaaatagt tgaatgcctc
     3481 ggcagggctg aggcgtcgag cgaacgaacc gatcctgcga cacaagcgcg gaacggtccg
     3541 ctcagcggcc ttgcgcag
//
LOCUS       DATLJT010000958         4753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4429486,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000958 DATLJT010000000
VERSION     DATLJT010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4429486"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1707)
                     /locus_tag="VM910_22005"
     CDS             complement(67..1707)
                     /locus_tag="VM910_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65230.1"
                     /translation="MTTQSTVITSASPATRESWVPMIAIALGQMIMSFNVASLPVALS
                     GMVHSFGVPPTTVATGIVAYGMLVAGFVMLGAKLIQRFGALYVFRIAVIMFCASQALM
                     TFSPNAALMITAQALCGASAAVIVPSLVALIAENYSGQQQATAIGALGSARAAAGVLA
                     FLIGGILGTYIGWRPVFGILIAASALVFLLSFRLRRDAGRHDVQIDAVGVVLAAAAII
                     LISFGFNNLNGWGLGVATANAPFSLFGLSPAPILIVLGIVLGQAFLMWTHRRQAAGKT
                     PLLDLAVIDSPRERAAVYAMFAVVALEAMLNFTVPLYIQIVQGRSPLATAVAMLPFNL
                     TVFFAAMLIVKLYDRLTPRQIGRYGFALCTVALLWLAFVVRNDWSEVPVLFGLVLFGI
                     GQGSLVTLVFNVLVTASPKELAGDVGSLRGTTQNLASAVGTAVSGALMVGLLSVIILR
                     SLAGHPVLTPELQSQVDLDSINFVSNDHLKGVLEQTTATPVQVEEAVRVNTEARLRAL
                     KIGLLIMAGLAFLTIIPAGGLPDYRPGEIPDPQTGKRT"
     gene            complement(1768..2772)
                     /gene="glsA"
                     /locus_tag="VM910_22010"
     CDS             complement(1768..2772)
                     /gene="glsA"
                     /locus_tag="VM910_22010"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HVI65231.1"
                     /translation="MQKQAELSEEVGFPYISTGHLPDHQHVTALVDEAYARYKSNREG
                     ENSQVYPALARVPNDLFGICVVGTTGDIYAVGDTDYAFSIMSVSKPFVFALVCQEIGA
                     EQAREKLGANATGMPFNSLAAVERSPDGRTNPMVNAGAIATTSLVPGDTFDAKWRFIH
                     DGLSRFAGRKLPLNEEVYASASETNHRNQGIARLLQSFGRIYFDPAEATDLYTKQCSL
                     NVSAKDLALMGATLADGGVNPLTKERVVEPAVCHYALAVMTTAGLYETSGDWLYNIGL
                     PGKSGIGGGIVTVSPGKGGLGTFAPPLDRAGNSIKGQLVAKFLSQRLGMDLFVSKPEA
                     "
     gene            complement(2988..3986)
                     /locus_tag="VM910_22015"
     CDS             complement(2988..3986)
                     /locus_tag="VM910_22015"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65232.1"
                     /translation="MSHVRRREFITLIGGAVALPFTARARQGERMRRIGVLMPEAEND
                     PETPAWVAAFQQSLEKLGWSVGRNILIDYHWYLGEVERARAAITQMLPHAPDLILAVG
                     SPAVVVAQEATRTIPVVFVGVSEPIAQGFVASLARPGGNISGFTNLERSLGAKWLEVL
                     EEIAPHIARVAFVFNPDTALYAASFFPAAKAAASKYAVELVAATVHQIADVEAVTTML
                     AHDPGGGLIFPPDPFFTAERKSIVELAARYRLPAIYPFQFFPADGGLVSYGVNIPAQF
                     QQAAAYVDRILRGAEPSNLPVQQPTKLELVINLKTAKTLGLEIPPTLLALADEVIE"
BASE COUNT          954 a         1455 c         1463 g          881 t
ORIGIN      
        1 cggggcggcg agcagggcga tgcgcacatc accagcgtcg ccgccagccg cgcgcagtgc
       61 cgcccattac gtgcgcttgc ccgtttgcgg gtccgggatc tctcccgggc gataatcggg
      121 cagccccccg gccgggatga tcgtcagaaa cgccaatcct gccataatga gcaggccgat
      181 cttcagcgcg cgcaatcgtg cttcggtatt gacgcggact gcctcttcga cctgcacggg
      241 ggtggccgtg gtctgttcca gcacgccctt aagatggtcg ttgctgacga aattgatgct
      301 gtcgagatca acctgcgact ggagctcggg ggtcagcacc gggtgtccgg ccaagctgcg
      361 caggatgatc acgctgagca gcccgaccat cagagcgccg gacaccgccg ttcccacggc
      421 ggacgcgagg ttctgagtgg tcccgcgcag cgacccgacg tcacccgcca gctctttggg
      481 cgatgcggtc acgagcacat tgaacaccaa ggtgaccaac gaaccctgtc cgatgccgaa
      541 caggacgagg ccgaaaagca cgggaacttc gctccaatcg tttcgcacga caaaagcgag
      601 ccagagcagc gccacggtgc agagcgcgaa gccgtagcga ccgatctgtc gaggcgtcag
      661 tcgatcgtag agtttgacga tcagcatcgc cgcaaagaac accgtgaggt tgaacggcag
      721 catggcgacc gcagtggcga gcggcgaacg gccctgtacg atctggatgt acaacggcac
      781 ggtgaaattg agcatcgcct cgagcgcgac caccgcgaac atggcgtaga ccgcggcccg
      841 ctcccgcggc gagtcgatca cggcgagatc aaggagcggt gtttttcccg cagcttggcg
      901 cctgtgcgtc cacatcaaga acgcctgccc gagcacgatg cccagcacga tcaggatcgg
      961 cgccggcgac aggccgaaaa gagagaacgg cgcgttggcg gtggcaacgc ccagtcccca
     1021 gccgttgaga ttgttgaagc caaaactgat caggatgata gcggcggcgg ccaaaaccac
     1081 gccgactgcg tcgatttgca cgtcgtggcg gcctgcgtcc cgcctcagcc ggaagctcag
     1141 aaggaacacg agcgccgagg cggctatgag aattccgaat accggccgcc agccgatata
     1201 cgtgcccagg atgccaccga tcagaaatgc cagcacgccg gccgccgcgc gcgcggaacc
     1261 gagcgcaccg atagcggtcg cctgctgttg gccgctgtag ttttccgcga tcaacgcgac
     1321 cagcgatggc acgatcacgg cggcggaagc tccgcacagt gcctgtgccg tgatcatcag
     1381 cgcggcattg ggactgaacg tcatcaacgc ctgcgaggcg caaaacataa tgaccgcaat
     1441 gcgaaaaacg tacagcgccc cgaagcgctg aatcagtttt gcgccgagca tcacgaagcc
     1501 ggccaccagc atgccatacg cgacgatacc ggtcgccacg gtggtcggcg gaacgccgaa
     1561 gctgtgcacc atccctgaca gcgcaaccgg caaggacgcg acgttgaagg acatgatcat
     1621 ctgcccgagc gcaatcgcga tcatcggcac ccaggattcc cgtgttgccg gggatgcgct
     1681 cgttatcacg gtagactgtg tcgtcatcgc ttgcctcccc aatcgaattg tcattggtcc
     1741 gagccggcgc aagcggcgtc tcgccgatca ggcctcaggt tttgagacaa acagatccat
     1801 gcccagccgc tgcgacagga acttggccac gagttgcccc ttgatgctgt ttccggcgcg
     1861 gtcgagaggc ggagcgaatg tgcccagtcc gcccttgccc ggagacactg tgacgattcc
     1921 gccgccgata ccgctcttgc cgggcaggcc gatgttgtag agccagtcgc cagaggtctc
     1981 gtacaagccg gctgtcgtca tcactgccag cgcgtaatga cagacggccg gctcgacgac
     2041 gcgctccttg gtgagcggat tgacgccgcc gtccgccagc gtcgctccca tgagcgcgag
     2101 gtcctttgca ctcacgttga gcgagcattg cttggtgtag agatcagtcg cctcggcagg
     2161 gtcgaagtag atgcgaccaa aactctgaag cagacgcgca atcccctggt tgcggtgatt
     2221 tgtttccgag gcggacgcat acacctcctc gttcaaagga agctttcggc cggcgaaccg
     2281 cgagagaccg tcatgaatga aacgccattt cgcatcgaag gtgtcgcctg gcacgaggct
     2341 cgtggtagcg atggcgccgg cattgaccat cggattcgtt cggccatctg gactgcgctc
     2401 gacggcagcc agcgaattga acggcattcc cgtcgcgttc gcgccaagct tctcgcgcgc
     2461 ttgctccgcg cctatttcct ggcagaccag ggcgaacacg aacggcttcg acacgctcat
     2521 gatcgagaac gcgtagtcgg tatctccgac cgcgtagata tcgccggtcg tgccgaccac
     2581 gcatatgccg aacaggtcat tcggtaccct ggcgagggcc gggtaaacct gcgagttctc
     2641 gccttcacga ttggatttgt atcgagcgta ggcctcgtcg actagcgcgg tcacatgctg
     2701 atgatccggc aggtgaccgg tcgatatata aggaaatccg acctcttcgc tgagctccgc
     2761 ttgcttttgc atttctcgcc gccttccgag gcgcttgggc aggccgatca cggatggaga
     2821 aaacaactgg aaatgagagg gccgccactc ggtttaactg agaggccgct tcaaggatcg
     2881 tttgttagtg gaagacgttg caatagagtc gtcaagaaaa caagctcgca agcgcactgc
     2941 gattcacgca aggctgattt gcgcaacggt gatgaattcg cgtcggttca ttcgatcacc
     3001 tcgtcggcga gcgcgagcaa cgtgggcgga atttcgagcc cgagtgtttt ggcggttttc
     3061 agattgatca ccaactcgag tttggtcggc tgctgaaccg gcagatttga aggctccgcg
     3121 ccgcggagta tccgatcgac ataagcggcc gcctgttgga attgcgcggg gatattgacg
     3181 ccatacgaaa ccaaaccgcc gtcggcagga aaaaactgga atggatagat cgccggtagc
     3241 cggtatcgag ccgccagttc gacgatcgat ttgcgctccg ccgtgaagaa ggggtccggg
     3301 ggaaaaatca aaccgccgcc ggggtcatgg gcaagcatcg tcgtcaccgc ttcgacgtcc
     3361 gcgatttgat gcaccgtggc tgcaactagc tcgaccgcat attttgaagc ggccgccttc
     3421 gccgcaggga agaatgatgc ggcatagagg gccgtatccg gattgaacac gaatgcgacc
     3481 cgggcgatat gtggtgcgat ctcctcgagc acctccagcc attttgctcc taagctccgc
     3541 tccaaattcg tgaagccgct aatgttgccg ccggggcggg cgagactcgc aacaaaaccc
     3601 tgggcgatcg gttcgctgac tccgacgaat acgacgggga tggtccgggt cgcctcctgc
     3661 gccactacca ccgccgggct gccaaccgcc agaatgaggt cgggggcgtg cggcagcatt
     3721 tgcgtgatgg cggctcttgc ccgctcaacc tcgcccaagt accagtgata atcgatcaga
     3781 atattgcgac cgactgacca ccccagcttt tcaaggctct gctgaaacgc tgcaacccac
     3841 gccggcgttt ctgggtcgtt ctcggcctcc ggcatcagca ctccaatccg gcgcatccgc
     3901 tccccctgtc gtgcgcgggc ggtgaacggc aacgcgaccg ccccgccgat gagcgtgatg
     3961 aactcgcgcc gtctcacgtg ggacatgggg cttctcctcg acgttatgcg tctgcgctac
     4021 accggcgacc gccctcaaga acaacgagct ttcgcatgga gacctcccgt ttgttggttg
     4081 atctcgccgc gcggcggcgc gctggtggct ggtttgcgcg agaaagtgcc ttaaccacgc
     4141 tcattacgcg ggaccggccc ccggaccgct gaaccgcaaa gaatgcgata tcggttcact
     4201 tgctcgcgcg atgtcgactg tcttctgtac gatctccacc tccgtggtct tgtagtgaag
     4261 tagacgttcg agcttgcgga cctcccgctc gatttccgag aagggctcag acgtttcaac
     4321 ggcaacactc atgatcaggc ctcatccgtt tgtggttcag tggtaggtta caaagcgctc
     4381 ggccgtaaac ttgctacaaa agccaagaac gcgatgagaa gagtgcgggt gccggagctg
     4441 tcgggcggcc gcgcatgcgc cgtgttggca agcgttggca gacatgtgcg gcaggttcga
     4501 agcgccgcga tgccttcgat tcatgttgtc ctgccggacc cgatattcgc tctcgcgggt
     4561 gcgggacctg aggtagtacg ctcgcctctt tcaggccgat gtggcgcgat gaagcccaaa
     4621 cgtcgctccg tcgtatcgtg gcatttgcag accaaatgct tcacgctttg gacgcgtgac
     4681 gcctgagcga attccgcagg atacgtgtcg ggtcggttgt tctcggaact gacgccgagg
     4741 aactgatggg cta
//
LOCUS       DATLJT010000959         8284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4429771,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000959 DATLJT010000000
VERSION     DATLJT010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4429771"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VM910_22020"
     CDS             complement(<1..506)
                     /locus_tag="VM910_22020"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HVI65233.1"
                     /translation="MLRALGLAQHRVAQLFGVGPKSVRRWQHGDRRVPCGVSIVLRLL
                     AAGAVTVAQIEQAAVPVPARANGSAKPESPAPLLVEPAPTPATLVRGEAATFADSDLT
                     TAEKVIALAPGACRWPCGDPGHPDFHFCGDPVAKRPYCEHHRTMAYMAPRTGGGHGAR
                     IRLVAQWG"
     gene            795..1982
                     /locus_tag="VM910_22025"
     CDS             795..1982
                     /locus_tag="VM910_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65234.1"
                     /translation="MQPRYWPDDADVYGLIFLPRFIDKIGSRVFADWTGTEVSLSEVD
                     LLPELTEANIGDRIIASSLLKEHRPDLGQQDFISSPWSAADNARRHELWRIAQQLAER
                     ERNAPSRCGLRCIKAEIRRQSCAGELTLKIRRVSGGPFEAFQPVWWNRDNPNELFDCY
                     IIDPEYPFGERPSWKPNTDSWIFAPREGIDQIAARLTAPTEAEPQLPRSAIKLDPVVQ
                     ASAYAQSAPEQPEAPPPAPAAEAAPAALKPIELATTEPAPAESPAVLPARGNEPSPAQ
                     SAFEIQSETPPPATQPNEQTVADSAPGDSEPAAAKKRVKRQKRVKRQAKKQVKRRGPD
                     RFSESDRALFLVMTKLMKEEQLSAQEAAQRLGDLGKIEGRGGADSRVLRLARRYRKEV
                     LKQ"
     gene            2102..2350
                     /locus_tag="VM910_22030"
     CDS             2102..2350
                     /locus_tag="VM910_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65235.1"
                     /translation="MSFAVRSELDNLRERVKKLEEVARRTRRGRTNQLGAARYLGKSR
                     EWLRKLHLRGEGPHRNPDGSYSFDDLDSSSEKGGPPSQ"
     gene            2407..2592
                     /locus_tag="VM910_22035"
     CDS             2407..2592
                     /locus_tag="VM910_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65236.1"
                     /translation="MNNILTDHILVDLIQQLDAVRDLHEQADTADALQRAQTLVDDAL
                     RQVTNFLFELRRKQLGI"
     gene            2595..2867
                     /locus_tag="VM910_22040"
     CDS             2595..2867
                     /locus_tag="VM910_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65237.1"
                     /translation="MSSQPPAPPSDEVLTPAPRLVRVMNGGACAGFLINLGPRGVEAF
                     DKDEKSLGVFPDAISAVTAVEKSVAPPDNVTRFIAHPAAHKSAGAS"
     gene            2864..3166
                     /locus_tag="VM910_22045"
     CDS             2864..3166
                     /locus_tag="VM910_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65238.1"
                     /translation="MNRERLPNRRAAETFEIEVNGLRYTATVSRFADGRIGELFLSNH
                     KSNSQADTNARDSSIALSFAVQHGADPRAIRCALCRDSHGRVSGPLGAALDLLLGG"
     gene            3163..3645
                     /locus_tag="VM910_22050"
     CDS             3163..3645
                     /locus_tag="VM910_22050"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVI65239.1"
                     /translation="MNNHEARLGVDPGISGGLAVVEITDGTAPGVVECIDIPVIGANA
                     KERADVIAIRAWISKHNVQHAFIERAQAMPHQGASSGFKYGRAVGATEAAIALSGIPV
                     TIIEPAVWKRAFCLPGKNKEAARQRALELFPAAHSLIARKRDHGRAEASLIALYGAKS
                     "
     gene            3642..4892
                     /locus_tag="VM910_22055"
     CDS             3642..4892
                     /locus_tag="VM910_22055"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI65240.1"
                     /translation="MNAPLTPHSPFGGSVAARVLHCPASVGLVQKVPAYLRKPSAYAE
                     RGTACHTALVHLIDENEDLESLVGKTINNYTITQDDVENALRPAFAYIDALLTPETEF
                     YLEHPVVFPAVAGAFGTPDLIVRSSDTIRVIDFKFGAGVRVLALYPDGDEDVLNAQLM
                     FYAAAARHSLPNFFTGVKDIILTIVQPVSIEPDAEMVSTVTVTHAELDEFGAVYPIAC
                     EEALSDTPRLKRGAWCRFCPAKPICPAHTGPLLDLAQFAVPTPAALAAPPAKEAYLQL
                     LADGLNLVDAVKDIRTALHDQAKRALENGDIVPGYALSAGRAERRWRDDESTAIAALE
                     SLGLGRDDIIAETMRSPKQVEIRAKVRGLKVPPELIASHRSGVSLVRCENARAPVPGR
                     GELVQLFNRALETFQEGGNHESIS"
     gene            4876..5637
                     /locus_tag="VM910_22060"
     CDS             4876..5637
                     /locus_tag="VM910_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65241.1"
                     /translation="MNQYRDQDRDSRDDHGRANDNRHEDKAIAPAPARGGLVSLSALT
                     AALNNVDLTSGGGSSGKPMLQFKSREGAGAPWTVGQKRNIPEDGSSWAVNPMTFMWGF
                     ICFGNDNKPLGERLVPVSQPMPDVTELPDHGFSWQPQWAADMKCLDGIDAGSEVVFKS
                     NTDGGIKAIKGLIEAVRDRLNGGQHGNKISPIVRLEKDSYQHSQYGRIWTPCLTIVGW
                     MSLDGPEPAPAPVSPPPAPASPPPAEQPRRRRVAG"
     gene            6029..7927
                     /locus_tag="VM910_22065"
     CDS             6029..7927
                     /locus_tag="VM910_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65242.1"
                     /translation="MIQAGISNLPTDLESASRALGGAGKQKDGKKLIKLFCVQGADPR
                     DYPEAWQRFLTYARRDVGEMRDVYRRTRPLPLAEWQQYWAFERINRRGVKLDMPYVHR
                     AAALAAEDAVASGRRLNELTNGAVTRVTQAQGIATWLYDNLADAAIRAILTVGVPADN
                     DNNDDDDDDDDDDDDDDDDDKAELSLTRDRVGRLLATLEVKRVNGGLSTDETKACEAA
                     TLRLFGAGASPKKFARLEAQQVDGVLRDQYRFSGGFQTGRMSSKAAQIQNLTRDVLGE
                     DGVAQAPLVDAIADGCSYAELAAAEPVDVPVVRKLALLVRPALIAGPGKVFVWGDWSA
                     IEARITPWLAASEGAERVLDIFRANDADPTLPDIYTIAAADIVHKDPSAIIKPERQIG
                     KVATLSLQFGGSVGALQDMALSYHIHLDAAEARRIVDAWREANPWAREFWGAHRGGES
                     FGLWGAAMRAWEIPGQITTAGRIAFVYREDYLGGALFMALPSGRLLTYPRPRWREVDV
                     LDQNDKPTGEKRTELSFRRAHGRAKLWHGTLAENATQGCAADILRTTVTRIETNPALA
                     FMPIRMTTHDEIVCEVDEARAEEAKAILRREMLTLPDWAAGLPLASDECSCSYYTKSK
                     AALRGGKL"
     gene            7924..>8284
                     /locus_tag="VM910_22070"
     CDS             7924..>8284
                     /locus_tag="VM910_22070"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HVI65243.1"
                     /translation="MNAPFLARVLRSYQQRAATYLYERDAAFLIAPLGAGKGAAALTA
                     LAELIRDKHRRHALVIAPKLVATTVWPQEVASWPHLQHLRVAVLDGSPEQRRALLTTA
                     ATREVTVIGVDLAPWLVG"
BASE COUNT         1641 a         2641 c         2512 g         1490 t
ORIGIN      
        1 tggccccatt gagcgacgag cctgatgcgg gcgccatgcc cgccgccagt ccgcggcgcc
       61 atgtaggcca tggtgcggtg atgctcgcaa tagggcctct tggcaactgg atcaccgcag
      121 aagtgaaaat cagggtgccc aggatcgcca cacggccagc ggcaggcccc cggggcaagc
      181 gcgatgacct tctcggcggt ggtgagatct gagtcggcga aggtggctgc ctcgccgcgc
      241 accaaggtgg ccggtgtcgg cgccggctcg acgaggaggg gagcaggcga ttcaggtttg
      301 gcgctgccgt ttgcccgggc ggggacaggc acggcggcct gttcgatctg agcaaccgta
      361 accgccccgg cggccaggag gcggagcaca atgctgacgc cgcatgggac gcggcgatcg
      421 ccatgctgcc agcgtcggac gctctttggg ccgacgccga acagctgagc aacgcgatgt
      481 tgcgcgaggc cgagtgcgcg gagcatctcg cagagctcgc tcgcagctgc ggcgcgcggg
      541 tatcgccccc agccagaggg gcaggacggt cgttccgttg gcgcgaccag gtgaatggtc
      601 atcgcgcgcc cctgttgctg cagatgccat ccttgtcaat agacttgatg agcgtctaaa
      661 acaaaaccgc ggcgctgcgc gcgccgcggt ttctttaaac agccgacgcc gagcagggca
      721 acggccgcta aaggcttcga cagccacttg tagcgcgctg ttctgtccct gccaagccca
      781 gcttggaggg tgttgtgcag ccgcgctatt ggccagacga tgctgacgtt tatggtctga
      841 tttttttgcc gcgattcatc gacaaaatcg gctctcgcgt cttcgccgat tggaccggta
      901 ctgaagtgtc cctaagcgaa gtcgaccttc tgccggagct taccgaggca aacattggtg
      961 atcgaatcat cgccagctcc ctgctgaagg agcaccgtcc cgatctcggg cagcaagatt
     1021 ttatctcatc tccctggagc gccgccgaca acgcgcgacg tcacgagctt tggcgcatcg
     1081 cccaacagtt agcggagcgc gaacgcaatg caccctcgcg atgcgggcta cgttgcatta
     1141 aagctgagat cagacggcag tcctgtgcag gagagctcac gctgaagatt cgtcgcgttt
     1201 cgggaggccc gtttgaggcg ttccaacccg tgtggtggaa cagagacaat ccgaacgagc
     1261 tcttcgactg ttacattatc gatccggagt atcccttcgg cgaacgacca agttggaagc
     1321 caaacaccga ttcttggatc tttgcaccgc gcgaaggaat cgatcaaatc gcagctcgct
     1381 taacagcccc tacggaagct gaaccgcagt taccgcggag tgcgatcaag cttgatccgg
     1441 tagtccaagc ctcagcatat gctcaaagcg cgccagaaca accagaggca ccgccgcctg
     1501 ctcctgcggc agaggcagca cctgctgcac tgaagccgat cgagctggca actactgagc
     1561 cagcccccgc ggagtcgccg gccgtcttgc cggcaagagg caacgagccg tcgcccgctc
     1621 aaagcgcgtt cgagatccag tcggagacgc cgccacctgc gactcaacca aacgagcaga
     1681 cggtcgccga ttcggcgcct ggcgactctg aacctgccgc ggccaaaaaa cgggtgaagc
     1741 ggcagaaacg agtgaagcgg caggccaaga aacaggtgaa gcggcgaggc cccgaccggt
     1801 tcagcgagag cgaccgcgcg ctattcctag tcatgaccaa actcatgaaa gaagagcagc
     1861 tcagtgcgca agaggctgct cagcgattag gcgatctcgg taaaattgag ggacgaggcg
     1921 gcgccgacag ccgagtgctt cgcctagccc ggagatatcg caaagaagtc ctaaaacagt
     1981 aacttcctcg cgcttcctcc agcttctaca aacttcctcg caaacttcct cgtcgagtcc
     2041 aacagatacc cgcatagcgc caaggcggcc aaccttggtc aaactatgcg gagtataggt
     2101 catgtccttt gcagtccggt ccgaactcga caatctccgc gagcgcgtga agaagctcga
     2161 agaggtcgcc cgccgcacca ggcgcggtcg cactaatcag ctcggcgcag ctcggtatct
     2221 cggaaaatcg cgcgagtggt tgcggaagct tcacctgcgc ggtgaaggtc cgcaccgcaa
     2281 tcctgatggc agctacagct tcgacgatct cgacagctct tccgagaagg gggggccacc
     2341 tagtcaataa acgttgagct cggccggcgt accgcaaatg ccttccccaa cgctggagat
     2401 cctgccgtga acaacatcct caccgatcat atcctggtcg atttgatcca gcagctcgat
     2461 gctgtccgcg atctccacga gcaggcggat accgccgatg cgctccagcg cgcgcagact
     2521 ctcgtcgatg atgcgctgcg tcaggtgacc aattttctgt ttgaactgcg ccgtaagcag
     2581 ctaggcattt aaccatgtcg agtcagcccc ccgcgccccc aagtgatgaa gtgctgacgc
     2641 cggctccacg cttggtccgt gtcatgaacg gcggcgcctg tgccggattt ctcatcaacc
     2701 tcggcccgcg cggcgtcgag gcattcgaca aggacgaaaa atccctcggt gttttccccg
     2761 acgcgattag cgccgtaacc gcggtcgaga aatcagtcgc gcctcctgac aacgtaactc
     2821 gcttcatcgc gcacccggcg gctcacaaat cggcgggggc gtcatgaatc gtgaacgcct
     2881 cccgaaccgg cgcgcagccg agacgttcga gatcgaggtc aacggcctgc gctacaccgc
     2941 gaccgtaagc cggttcgctg atggtcgcat cggcgaactg tttctctcaa atcacaaatc
     3001 aaactcgcag gcagacacca acgcccgcga ttcaagcatc gcgctctcgt tcgcggtcca
     3061 acacggcgcg gatccgcgcg caatccgttg cgcgctttgc cgcgattcac atggtcgcgt
     3121 ctctggtccg ctcggggccg cgctcgatct cttgctgggg ggatgaacaa ccatgaggct
     3181 cgcctaggcg tcgatcctgg cattagcggc ggattggctg tcgtcgagat taccgacgga
     3241 accgcccccg gggtggtgga atgcatcgac attccggtga tcggcgcgaa cgcaaaagag
     3301 cgcgccgatg tgatcgcgat tcgcgcgtgg atcagcaagc acaacgtcca gcacgccttc
     3361 atagaacgcg ctcaggcaat gccgcatcag ggcgccagta gcggtttcaa gtatgggcgc
     3421 gccgtcggcg ccaccgaagc ggcgatcgcg ctgagtggga ttccggtgac catcatcgaa
     3481 cccgcggtct ggaaacgtgc tttctgtttg cccggcaaga acaaggaagc ggcgcgtcaa
     3541 cgcgcgctcg agctcttccc cgccgcgcat agcctgattg cccgcaaacg cgatcacgga
     3601 cgcgccgagg cgtcgttgat cgcgctctac ggagccaagt catgaatgcc ccgctgacgc
     3661 cgcactcacc attcggtggc agcgtcgcgg cgcgtgtcct tcactgcccg gcatccgtcg
     3721 gcctcgtcca gaaagtgcct gcctatctac gcaagccatc cgcctacgcg gaacgcggta
     3781 cggcgtgcca tacagcgctg gtccatctca tcgacgagaa cgaggacctc gaaagcctcg
     3841 tcggcaagac gatcaacaac tatacgatta cgcaggacga cgttgagaac gcgctgcggc
     3901 cggctttcgc ctacatagat gcgctcctga cgcccgaaac cgaattttat ctcgagcatc
     3961 ctgttgtttt tccagccgtc gctggcgcct ttggtacccc cgacctcatc gtccgtagca
     4021 gcgacacgat tcgcgtgatc gacttcaagt ttggcgccgg cgtgcgtgtc ctcgcgctct
     4081 accctgatgg cgacgaggac gtcctcaacg cacaactcat gttctacgcc gcagctgcgc
     4141 gtcattccct tcctaacttt ttcaccggcg ttaaagacat catccttacg atcgtgcagc
     4201 ccgtgtcgat cgagccggac gccgagatgg tctcgaccgt tacggtcaca cacgccgagc
     4261 tggacgaatt cggcgcggta taccccatcg cttgcgaaga agcactttca gacacaccgc
     4321 gcttgaagcg aggtgcgtgg tgccggttct gccccgcaaa gccgatctgc ccggcgcaca
     4381 ctggtccgct cctcgatctc gctcagttcg cggtgccgac gcctgccgcg ttggccgcgc
     4441 cgcccgcgaa ggaagcttac ctgcaactgc tcgccgacgg gctgaacctc gtcgacgcgg
     4501 tcaaagacat tcgcaccgcg ctgcacgatc aggcgaagcg cgcactcgag aacggcgaca
     4561 tcgttcctgg ctacgcgctc tcggccggcc gcgccgagcg ccgttggcgc gacgacgaaa
     4621 gcactgcaat cgccgcgtta gagagcctcg gcctcggccg cgatgacatc atcgccgaga
     4681 cgatgcgctc accaaaacaa gtcgagatcc gcgcgaaagt gcgcggtctc aaagtccccc
     4741 cagaactcat cgcttcgcac cgctccggcg tctcgctcgt gcggtgtgag aacgcacgcg
     4801 ctccggtgcc tggccggggc gagctcgtgc aattgttcaa ccgggcgctc gaaaccttcc
     4861 aggagggagg taatcatgaa tcaatatcgt gaccaagatc gtgacagccg tgatgaccac
     4921 ggtcgcgcta acgacaacag gcatgaggac aaggcaattg ccccggcccc agcgcgaggc
     4981 ggactcgttt cgctgtcggc actcactgcg gcactcaata acgtcgacct gacatccggc
     5041 ggcggtagtt caggcaagcc aatgttgcaa tttaaaagca gagagggcgc cggtgccccc
     5101 tggacggtcg ggcagaagcg taacatcccc gaagacggca gctcgtgggc cgtcaacccc
     5161 atgactttca tgtggggctt catctgcttc ggtaacgaca acaagccgct tggggagcgc
     5221 ctcgttcccg tcagccagcc gatgcccgac gttacggaac ttccggacca cggcttctcg
     5281 tggcaaccgc aatgggccgc cgacatgaag tgcctcgacg gcatcgatgc tggtagcgag
     5341 gtggtcttca agtcgaatac cgacggcggc atcaaagcta tcaagggatt gatcgaagca
     5401 gtgcgcgacc gactcaacgg cggccaacac ggcaacaaaa tctcgccgat tgtgcgtctt
     5461 gagaaggatt cgtaccagca cagtcaatac ggtcgtattt ggacgccgtg cctgacgatc
     5521 gttggttgga tgtcgctcga cggtccggag ccagcaccgg cgccggtatc gcccccgccc
     5581 gcgccagcgt cgccgccgcc tgccgagcaa ccgcgtcggc gtcgcgtcgc cgggtaacgc
     5641 cgatgcgcac ctgacaagcg cggccgacct gcaggcggcc tcgcctgacc ttttgagggt
     5701 ccgcgcgatg ttcaacgtca gcgatctcat ttggctggat ttcgagtcgg ccggcgcgct
     5761 cgacctgaag gccgccggaa ctctccgcta cgccaccgag ccttcaacgc gcgcaatcgt
     5821 tctggcctat gccatcggcg acgcgccggc gctcacttgg cacgccgacg gcgcgatcct
     5881 ggactggaac cacccgccaa atgatcttcg cgccgccttc gatcgcggca tgatcttcgc
     5941 tgcatggaac gcgagtttcg atagcgccat ctggaattat gcaacgctcg gattcccctt
     6001 cctcgcgccg gagcgcgtca tcgatccgat gatccaggcc ggcatctcca acttgccgac
     6061 cgatcttgaa agcgcctcgc gcgcgcttgg cggcgccggc aaacagaagg acggcaaaaa
     6121 gctgatcaaa ctgttttgcg tccaaggtgc agaccctcgc gactatccag aagcatggca
     6181 gcgctttctt acttatgcgc gccgggacgt cggggagatg cgcgatgtct accgccggac
     6241 gcgcccactg ccactcgcag aatggcaaca atactgggcg tttgaacgca tcaaccgacg
     6301 gggcgtgaag ctcgacatgc cctacgtgca ccgcgccgcg gctctcgctg ccgaggatgc
     6361 tgtcgccagt gggcgtcggt tgaacgagct gaccaacggg gctgtcaccc gcgtcaccca
     6421 ggcccaaggg atcgcgacct ggttgtacga taaccttgct gacgcggcga tacgcgcgat
     6481 tctaacggtt ggcgttccgg cggacaacga taacaatgat gatgatgatg atgatgatga
     6541 tgatgatgat gatgatgatg atgatgacaa agcggaactc agcctgacac gcgatcgcgt
     6601 cggccgcctg ctcgccacgc tcgaggtgaa gcgcgtcaat ggcggcctta gcactgacga
     6661 aacgaaagcc tgcgaagctg cgacgttacg cttgttcggc gccggggctt cgccaaaaaa
     6721 gttcgcacgc cttgaagcac agcaggtcga tggcgtgctg cgggaccaat accgattcag
     6781 tggcggtttt caaactggac gcatgtccag taaagccgcc cagatccaga acctcacccg
     6841 cgacgtgctt ggcgaggatg gtgtggcaca ggcgccgctc gtcgatgcga tcgccgatgg
     6901 ctgcagctac gccgagctgg ccgcggcgga gcctgtcgat gtaccggtag tgcgtaaact
     6961 tgctttgctt gtacgtccgg ccctcattgc cggaccggga aaggtctttg tgtggggcga
     7021 ctggagcgcg attgaagcgc ggattacgcc ctggctcgcc gcttccgagg gtgctgaaag
     7081 agtcctcgat attttccgcg ccaacgacgc cgatccgacg ctgcctgaca tttataccat
     7141 cgctgctgcc gacatcgtgc acaaggatcc gagcgcgata atcaagcctg agcggcagat
     7201 tgggaaggta gcaacgttga gtttgcaatt tggcggctcc gtcggtgcgc ttcaagacat
     7261 ggcgctgagt tatcatatcc acctcgacgc cgccgaggcg cgtcgcatcg tcgatgcctg
     7321 gcgcgaggcg aatccgtggg cgcgagaatt ctggggcgcc caccgcggcg gggagagctt
     7381 cgggctgtgg ggcgctgcga tgcgcgcctg ggaaatccct ggccagatca ccacagcagg
     7441 ccggatcgcc tttgtctatc gtgaggacta tctcggcggc gcgctgttca tggcgctgcc
     7501 gtcaggtcgc ttgttgacct atccgcgacc gcgttggcgc gaggtcgatg ttctcgacca
     7561 gaacgataaa ccgaccggcg agaaacgcac cgagctgtcg tttcggcgcg cccacggacg
     7621 cgcgaagtta tggcacggca cgctggcgga gaatgccacc cagggatgtg ccgccgatat
     7681 cctgcgcaca acggtgacac ggatcgaaac gaatccggcg ctcgcgttta tgccaatccg
     7741 catgacgaca cacgacgaga ttgtctgcga ggttgatgag gcgcgcgccg aagaggcgaa
     7801 agcaatcctg cggcgcgaga tgctgacgct tccagactgg gccgcaggcc tgccgctcgc
     7861 atcagatgag tgctcgtgct cgtattacac gaagagcaaa gccgcgcttc gcggaggcaa
     7921 gctatgaatg cgcccttcct cgcgcgcgtc ctgcgtagct atcaacaacg ggcagcaaca
     7981 tacctttacg agcgcgatgc tgcgttcttg atcgcgccgc taggcgccgg caaaggcgcc
     8041 gctgcgctta ctgcgcttgc cgagttgatc cgcgacaagc atcggcgtca cgctcttgtt
     8101 atcgcgccca aactcgtggc gaccaccgta tggccgcagg aggtcgcgtc ctggccgcac
     8161 cttcaacacc ttcgcgtcgc cgttctcgac ggcagccccg agcaacgcag ggcactcctc
     8221 actaccgctg cgacgcgaga ggtgacggtg attggggtcg atctcgcgcc ctggctcgtc
     8281 ggcg
//
LOCUS       DATLJT010000960         6489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1902625,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000960 DATLJT010000000
VERSION     DATLJT010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902625"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VM910_22075"
     CDS             <1..453
                     /locus_tag="VM910_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65244.1"
                     /translation="WSADPNIRDFIGLSENSWDFNAPGAMAGFGPLEMTEELRRQVVR
                     IVGRDLAGDPTDPAGPIAAEPQGKSSSAEIEISTKSAATAAEAPAQQAQSSAPTPQDE
                     STEKHNERHNSDELPQRNKAAIASQQSPGNSDNDQLIAKRPHGRALPK"
     gene            546..1325
                     /locus_tag="VM910_22080"
     CDS             546..1325
                     /locus_tag="VM910_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HVI65245.1"
                     /translation="MRDVGLDEAIRVAGGVGALARKIGISQPSVSNWLRVPAERVLSV
                     EAATGVSRAMLRPDLYAEHPGTAGDVDEVDAARAQEYALLSVLLARAPDAALLERLGA
                     LRGDASPLGLAHVALAEAAQRTNVERVEREYFDLFIGIGRGELLPYGSYYLTGFLHER
                     PLARLRAHLGKLAIERTDGQAEPEDHAAILCEIMAGLASRRFPAPVGSDRELFEQHLA
                     PWIGRFFADLEQAEAADFYRGVGRLGRVFIDIETEAFALPS"
     gene            1365..1574
                     /locus_tag="VM910_22085"
     CDS             1365..1574
                     /locus_tag="VM910_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI65246.1"
                     /translation="MKRERKTTLGRREFLRTLGAGATVAAAAATPLADEARADTESND
                     EKRKPRYQANSPEVQTFYRVNRYPS"
     gene            1640..4543
                     /locus_tag="VM910_22090"
     CDS             1640..4543
                     /locus_tag="VM910_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HVI65247.1"
                     /translation="MQGPSDGALDRRAFLRRSGLVAGGLATLGALPLASVRKAEAGPP
                     PPAGAQVTIRKSVCTHCSVGCTVVAEVANGVWIGQEPGWDSPINRGSHCAKGASVREL
                     VHGDRRLRYPMKLVNGQWTRVSWDQAIGEIGDKLMAIREKSGPDSVYWLGSAKMTNEG
                     SYLFRKLGAFWGTNNTDHQARICHSTTVTGVANTWGYGAMTNSYTDIRNAKTLVFLGG
                     NPAEAHPVSLQHLLEGKELNRANFIVIDPRMTRTAAHATEYVRMRPGTDIPILWGMLW
                     HIFKNGWEDKEFIKQRVYGMDDARKEIEKWTPEEVERVSGVPGEQLKRVAEKFAKEKP
                     STLIWAMGQTQHTVGTANVRASCIALLATGNVGGPGMGANIFRGHDNVQGATDVGLDI
                     VTLPFYYGLLEGAWRHWGRVWEVEYDWLQSRFDSKKIMETAGIPLTRWFDAVLLPKEQ
                     VDQKDNVRAMFVQGHASNSITRIPESVKGMQKLDLLVVADPHPTTWASLAVNGGRRDG
                     TYLLPVCTQFEAKGSRVASNRALQWGEQIVKPIFESKDDLEVIYLVAAKLGFADKMFK
                     NIKVENNHPEAEDVLREMNRGSWSTGYCGQSPERLKLHMANQKDFDLLTQRATSGPAK
                     GDYYGLPWPCWGSPEFKHPGTPLLYNTNLSIKDGGGTFRARFGVERVVKTKVMENGQE
                     VEKEQKFNLLAEGSYSKDSEIKDGYPEFTLAVLKKLGWDKDLTEAEMDVIRKVNSANP
                     DAVSWSLDLSGGIQRVAITHGCIPYGNGKARANAFGLPDPIPVHREPIYTPRVDLVSK
                     YPTLPNAKQFRLPNVGFDVQKAAVDKGVAKDFPLILTSGRLVEYEGGGEETRSNKWLA
                     ELQQDMFIEINPADATDRGIKDGGWVWVTGAESGSRAKMKALVTERVGKGVTWMPFHF
                     GGWFMGADLRDKYPQGTDPVVLGESANTITTYGYDPATGMQEPKATLCQIRAA"
     gene            4556..5152
                     /gene="fdh3B"
                     /locus_tag="VM910_22095"
     CDS             4556..5152
                     /gene="fdh3B"
                     /locus_tag="VM910_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HVI65248.1"
                     /translation="MARMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTINDG
                     KPGERSVSMACMHCTDAPCQAVCPVNCFYTTADAVVLHSKDLCIGCGYCFYACPFGAP
                     QYPRVGNFGSRGKMDKCTYCAGGPEADGSLAEFEKYGANRLAEGKLPLCAEMCSTKSL
                     LAGDGEIIAQIYKERVVKRGYGSGAWGWKTAYRETIAS"
     gene            5283..6305
                     /locus_tag="VM910_22100"
     CDS             5283..6305
                     /locus_tag="VM910_22100"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HVI65249.1"
                     /translation="MAMLISRIRFIVGAMALALIVVVAAPASAQQRNPDSSVNPTASS
                     VKEEQLLQEFQRIQGRGTIPDTRSYVIEQPAGRDWRQFHQVTLRWVGAISIVGMLVLL
                     VAFYLVRGMVRLESGRSGRVLVRFTAFERFVHWMTATCFIILALSGLNITFGKPLLLP
                     LIGPEAFTTWSQWAKYAHNYLSFPFTLGVFMIFLMWIAWNVPNKVDVEWLKEGGGIVG
                     HKHPPADRFNAGQKMIYWIQMLAGGAMAVTGYLLMFPFYGTNIADMQLAQIVHGVVAM
                     LFVAVMIAHIYIGTIGMEGAFEAMWDGTVDVNWAKEHHSLWAEQEGAKGDVSAPPRQG
                     KMQPAE"
     gene            complement(6262..>6489)
                     /locus_tag="VM910_22105"
     CDS             complement(6262..>6489)
                     /locus_tag="VM910_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65250.1"
                     /translation="MTLARSVVLPEPLQPARPMMRMRHYSSLKRVPSDTLRHTPRHET
                     KAPGEVRRALEFRDALAATPPAAFFLAAVVR"
BASE COUNT         1273 a         1999 c         2083 g         1134 t
ORIGIN      
        1 tggtcggccg accccaacat ccgcgacttc atcgggcttt ccgagaattc gtgggacttc
       61 aatgcgccag gcgcgatggc gggtttcgga ccgctggaaa tgaccgagga gcttcgccgt
      121 caggtcgtgc gaatagttgg acgcgacctt gccggcgatc cgaccgatcc cgccggtccc
      181 attgcggcag aaccgcaggg taagtcctcg tcggctgaaa ttgaaatatc gaccaaatcg
      241 gccgccacgg ctgccgaggc gcctgcccaa caagctcaaa gcagtgcgcc gacgccgcaa
      301 gacgaatcga ccgaaaagca caatgaacga cacaactccg atgagctgcc gcagcgcaat
      361 aaagcagcta ttgcgtcgca acagagtcca ggaaactccg ataacgatca attgattgcg
      421 aaacggccgc acgggcgcgc tttgcctaag tgatacgggg gactcatagc ctccggctat
      481 tgaccattgc tcgctgcggg cggtactttt ctacagtaga attaaaataa aaacgaaaag
      541 cgccgatgag ggacgtggga cttgatgagg cgatccgcgt agccggcggc gtcggcgcgc
      601 ttgcgcgaaa aattggcatt tcccagcctt cggtatcgaa ctggttgcgc gttcctgccg
      661 agcgtgtgct gtcggtcgaa gccgcgaccg gcgtcagccg tgcgatgctg cggcccgatc
      721 tctacgctga acatcccggc acggccggcg acgtcgacga ggtcgacgct gcgcgggcgc
      781 aagaatatgc gttgctctcg gtgctgctcg cacgcgcgcc cgatgccgcg ctgctcgagc
      841 gtctcggggc attgcgcggc gatgcaagcc cgctcggact ggcgcacgtc gcgctcgcgg
      901 aggcagcgca gcgcaccaat gtcgagcgcg tcgagcgcga atatttcgat ctcttcatcg
      961 gtatcgggcg cggggagctt ttaccttacg gatcctacta tctcaccggc ttcctgcacg
     1021 agcggccgct cgcgcgcctg cgcgcgcatc tcggcaagct cgcaatcgaa cggacggacg
     1081 gtcaagcgga gcccgaggat cacgcggcga tcctatgcga gatcatggcc gggcttgcga
     1141 gccgccgctt cccggctccc gtcggttccg atcgcgaact gttcgagcag catctcgcac
     1201 cgtggatcgg gcgctttttc gccgatctgg aacaggccga ggccgcggat ttctaccggg
     1261 gagtaggcag gctcggccgg gtgtttatag atatcgaaac cgaggctttc gcgctgccct
     1321 cgtgagcagc gcggagggca gagaggacat aggaggagac ggcgatgaaa cgagaacgca
     1381 agacgacgct cggccgtcgc gaattccttc gcacgcttgg cgcaggcgcg accgtcgcgg
     1441 ccgcagccgc cacaccgctg gcggatgaag ctcgcgccga caccgagagc aacgatgaga
     1501 agcgcaagcc tcggtaccag gcgaactcgc cggaggttca aaccttctat cgagtcaacc
     1561 ggtatccgag ctgagggagg cagccgtgct gatcaagaga acggaacgtc acgctcgccg
     1621 cggcacgctt gccgccgccc tgcagggccc atcggacggc gcgctcgatc gtcgcgcttt
     1681 cctgcgccgc tccggcctcg ttgccggcgg tctcgccaca ctcggcgcgc taccgctcgc
     1741 gagcgtgcgc aaggcggagg cgggtcctcc cccgcccgcg ggcgcgcaag tcaccatccg
     1801 caagagcgtc tgcacgcatt gttcggtcgg ctgcacggtg gtggccgaag ttgcgaacgg
     1861 cgtgtggatc ggccaggagc ccggctggga ttcgccgatc aaccgcggtt cgcattgcgc
     1921 caagggcgcc tcggtgcgcg agttggtgca cggcgatcgg cgtctgcgct acccgatgaa
     1981 gctggtcaac ggccaatgga cgcgcgtgtc ctgggaccag gccattggcg agatcggcga
     2041 caagctcatg gcgattcgcg agaagtccgg gcccgactct gtctattggc tcggctcagc
     2101 gaaaatgacc aacgagggtt cctatctgtt ccgaaagctc ggcgcgttct ggggcaccaa
     2161 taataccgat caccaggcgc gcatctgcca ctccacgacg gtcaccggcg tagccaacac
     2221 ctggggctac ggcgcgatga ccaacagcta caccgacatc cgcaacgcca agacgctcgt
     2281 tttcctcggc ggcaatcccg ccgaagcgca cccggtatcg ctgcagcatc tgctcgaagg
     2341 caaggagctt aaccgggcca acttcatcgt cattgatccg cgcatgacgc gcacggccgc
     2401 ccacgcgacg gagtacgtgc ggatgcggcc agggactgac attcccattc tctggggcat
     2461 gttgtggcac atcttcaaga acggatggga ggacaaggag ttcatcaagc agcgcgtcta
     2521 cggcatggac gacgcccgca aggagatcga gaagtggacg ccggaggagg tggagcgcgt
     2581 cagcggcgtg ccgggtgaac agctcaagcg cgtcgccgag aagttcgcca aggaaaaacc
     2641 ttcgaccttg atctgggcga tgggacagac ccaacatacg gtcggcaccg ccaacgttcg
     2701 tgcgagctgc attgcactac tggccaccgg caatgtgggt ggtcccggca tgggcgccaa
     2761 catcttccgc ggccacgaca acgtacaggg cgcgaccgac gtcggactcg atatcgtcac
     2821 gctgccgttc tactacggcc tgctcgaagg tgcgtggcgg cactggggtc gcgtctggga
     2881 ggtcgaatac gactggctgc agtcgcggtt cgattccaag aagatcatgg agaccgccgg
     2941 gatcccgttg acccggtggt tcgacgccgt gctgctgccg aaggagcagg tcgatcagaa
     3001 agacaacgtt cgggcgatgt tcgtgcaggg ccacgccagc aacagcatta cgcgcatccc
     3061 cgaatccgtc aaaggtatgc agaagctcga tcttctggtc gtagccgatc cgcatccgac
     3121 cacgtgggcc tcgcttgcgg tgaacggtgg acgaagggat ggcacctatc tgctgccggt
     3181 gtgtacgcaa ttcgaggcca aaggctcgcg cgtggcgtcc aaccgggcgc ttcagtgggg
     3241 tgagcagatc gtcaagccca tcttcgagtc gaaggacgat ctcgaggtta tctatttggt
     3301 ggccgcgaag ctcggcttcg ccgacaagat gttcaagaac atcaaggtcg agaacaatca
     3361 ccccgaggcg gaagacgttc tgcgcgagat gaatcgcggc agctggtcga ccggctattg
     3421 cggccagtcg ccggagcggc tgaagctgca catggccaac caaaaagact tcgacctgct
     3481 cacgcagcgt gccacatcgg ggcccgctaa gggcgactac tacggcctgc cgtggccatg
     3541 ctgggggtcg ccggagttca agcatcccgg cacgccgctg ctttacaaca ccaatctcag
     3601 catcaaggac ggcggcggca cgttccgcgc gcgtttcgga gtcgagcgcg tggtcaagac
     3661 caaggtgatg gagaacggcc aggaggtcga aaaggagcag aaattcaatc tccttgccga
     3721 aggctcctac tccaaggatt ccgagatcaa ggacggctat cccgagttca ccctggcagt
     3781 gctgaagaag ctcggttggg acaaggacct cacggaagcc gagatggacg tgatccggaa
     3841 ggtgaactcc gccaacccgg acgcggtgtc gtggtcgctc gacctctccg gcggtatcca
     3901 gcgcgtcgcc atcacgcatg gatgcattcc gtacggcaac ggcaaggcgc gggcgaacgc
     3961 attcgggcta cccgacccga tcccggtgca ccgcgagccg atctacacgc cgcgcgtcga
     4021 tcttgtctcc aagtatccga cgttgccgaa cgccaaacag ttccggctgc cgaatgtcgg
     4081 tttcgacgtg caaaaggcgg cagtggataa gggagttgcc aaggactttc cgcttatcct
     4141 cacctcgggc cgtctggtcg agtacgaggg cggcggcgag gagacgcggt ccaacaaatg
     4201 gttggccgag cttcagcagg atatgttcat cgagatcaat cctgccgacg ccaccgaccg
     4261 cggcatcaag gatggcggct gggtctgggt taccggcgcg gagagcggtt ctcgcgccaa
     4321 aatgaaggcg cttgtcaccg aacgagtcgg caagggtgtg acgtggatgc ccttccactt
     4381 cggcggttgg ttcatgggcg ccgatttgcg cgacaaatat ccgcagggca cagaccctgt
     4441 cgtgcttggc gagagtgcga acacgatcac gacctacggt tacgacccgg caacgggcat
     4501 gcaggaaccg aaggccaccc tctgccagat cagagcggca taaggaggct cgacaatggc
     4561 tcggatgaaa tttctctgcg acgccgaccg ttgcatcgag tgcaacgctt gcgtcacggc
     4621 ctgcaagaac gagcacgaag tgccctgggg catcaaccgc cgccgcgtcg tcaccatcaa
     4681 cgacggcaag ccgggcgagc gctcggtctc catggcctgc atgcactgca ccgacgcgcc
     4741 ttgccaggcg gtctgcccgg tgaactgctt ctacaccacc gccgacgcgg tggtgttgca
     4801 ctccaaggac ctctgcatcg gttgtggcta ttgcttctac gcctgcccat tcggcgcgcc
     4861 gcaatatccc agggtcggca acttcggctc gcgcggcaag atggacaaat gcacttattg
     4921 cgcgggcggg ccggaagcag acggctcgct ggccgagttc gagaagtacg gcgccaatcg
     4981 tttggccgaa ggcaaattgc cgctctgcgc cgagatgtgc tcgaccaagt cgctgctggc
     5041 cggcgacggc gagatcatcg cgcagatcta caaggagcgc gtcgtcaagc gcggctatgg
     5101 ctcgggcgcc tggggctgga aaacggcata tcgcgagacg atcgcatcgt agaacgtgga
     5161 cgcccgttgc gcgtccagcc ctgggccatg gttgttcggt gcccgaggcg aacgccatga
     5221 atattggatt gtccgctgtc gcgaaggtcg cagaggatga cggccaacaa ggaggaaaca
     5281 ccatggcgat gctaatcagc cgcatccgtt tcatcgtcgg tgcgatggcg ctggcgctga
     5341 tcgtcgtcgt ggcagctccc gcgagcgctc agcagcgcaa ccccgacagc tcggtcaacc
     5401 cgacggcgag ctcggtgaag gaggaacagc tgctgcagga gttccaacgg atccaggggc
     5461 gcggaacgat ccccgatacc aggtcctacg tgatcgaaca gcccgctggc cgagattggc
     5521 gccagttcca ccaggtcacg ctgcgttggg tcggggccat ttcgatcgtc ggtatgctcg
     5581 ttctcctcgt tgccttctat ttggtccgcg gcatggtccg cctcgagtcg ggacggtccg
     5641 ggcgcgtgct ggtgcggttc accgccttcg agcgctttgt gcattggatg acggccacct
     5701 gcttcatcat cctcgccctt tccggactca acatcacgtt cggcaagccg ttgctgctgc
     5761 ccctgatcgg gccagaggca ttcaccacct ggtcgcagtg ggcgaaatat gcccacaatt
     5821 acctgagctt cccgttcacg ctcggcgtgt tcatgatctt cctgatgtgg atcgcctgga
     5881 acgtcccgaa caaggtggac gttgaatggc tcaaggaagg cggcggcatc gtcggccaca
     5941 agcatccccc cgccgatcgc ttcaacgccg gtcagaagat gatctattgg attcagatgc
     6001 tagccggcgg cgccatggca gtgaccggct atctcctcat gttcccattc tacgggacga
     6061 acattgcaga catgcaattg gcccagattg tccacggcgt cgtggcgatg ctgttcgtcg
     6121 ccgttatgat cgcccacatc tatatcggca ccatcgggat ggaaggcgcg ttcgaggcga
     6181 tgtgggatgg gacggtcgac gtcaactggg caaaagagca ccacagcctg tgggccgagc
     6241 aggaaggtgc caagggggat gtcagcgcac caccgcggca aggaaaaatg cagccggcgg
     6301 agtagcggcg agggcatcgc gaaattcaag cgcccggcgc acttcgccgg gcgctttcgt
     6361 ttcatggcgc ggcgtatgcc ggagggtgtc gctcgggaca cgcttgaggc tgctataatg
     6421 ccgcatgcgc atcatcggcc tcgccggctg gagcggctcc ggcaagacca cgctcctcgc
     6481 caaggtcat
//
LOCUS       DATLJT010000961         3082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1442942,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000961 DATLJT010000000
VERSION     DATLJT010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442942"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VM910_22110"
     CDS             complement(<1..657)
                     /locus_tag="VM910_22110"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI65251.1"
                     /translation="MQPRDRLSFSAITKRPALRFPSGIRLVVWPVLALEEWDISRPMA
                     RMVISPPQGQPQLPDMPNWSWHEYGMRVGFWRLRKMLGQVGAKPTVTLNARVCETYPE
                     VVEACVENGWELNAHSYDQVPMHKLDDQRGVIMKSMDIIEKFWGKRPRGWFGPGLTQT
                     YETLDYLAEAGVEYIGDWVLDDEPVTLKTTHGSVVALPYNFELHDIVMMALQHHPSEM
                     M"
     gene            complement(784..1158)
                     /locus_tag="VM910_22115"
     CDS             complement(784..1158)
                     /locus_tag="VM910_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI65252.1"
                     /translation="MTKILYVEDNDDNVYMLKMRLELLGDFDVVAAPDGEQGCAMALS
                     ERPDVILMDLEMPVVDGWEATRRLKDDPQTRDIPIIALSAHALAGEREKALAAGCDEF
                     DTKPIEFERLVATLRRVLAARE"
     gene            complement(1155..2111)
                     /locus_tag="VM910_22120"
     CDS             complement(1155..2111)
                     /locus_tag="VM910_22120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI65253.1"
                     /translation="MREGVPMGVFNLARVTVRPFTEKQIELVSTFADQAVIAIENVRL
                     FDEIQDKSRQLELASQHKSQFLASMSHELRTPLNAIIGLTEMMVTNAARFGTEKAAEP
                     LKRVHRAGTHLLGLINQVLDLSKIEAGKLQLNPESVSLAPLIDEVVGTPRQLAEQNKN
                     RLVVEAEEKLGALTVDPMRLRQILLNLLSNACKFTTQGEVKLRVRKVADGRNWVEFAV
                     ADTGIGMTAAQQAKLFEEFTQADASTAQRYGGTGLGLAITRKLARMMGGDVTVASEPG
                     KGSVFTVRLPGGGGVHARSSSEDIKSQAGDAGARDNGMEAAS"
     gene            complement(2111..>3082)
                     /locus_tag="VM910_22125"
     CDS             complement(2111..>3082)
                     /locus_tag="VM910_22125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI65254.1"
                     /translation="HIELVTNFAAQAVIAIENTRLLNELRESLQQQTATAELLQIINS
                     SAGELASVFDAILEKALSVSDAAFGGLWTYDGEHMHAVAMRGVPALFAAFHNKPLDPV
                     PGTGSYALVRGESFVHIPDITDDEGYRSGNPGKRALAELGGARTALWLPLRKDQMLLG
                     TFMVYRQEVRPFNDKQIALLQNFAAQAVIAMENARLLNELRQSLQQQTATADVLKVIS
                     RSTFDLVTVLETLVASAARLCDADKGFIARREGVGYRLAANFGFAPEFVEYVGQVLIE
                     PGRNTLVGRTALEGRTVHIPDIRTDPELPGPRRSSAVEAFAPSWEYR"
BASE COUNT          558 a          970 c          932 g          622 t
ORIGIN      
        1 catcatctcg gatggatgat gctgcagagc catcatgacg atgtcgtgaa gctcgaagtt
       61 gtaagggagc gccacaacgg acccatgcgt cgtttttagt gtcacgggtt catcgtcgag
      121 aacccagtca ccgatatatt caacgccggc ctccgcgagg taatcgagag tctcgtaggt
      181 ttgcgtcagg ccaggcccga accagccacg cggtcgctta ccccagaatt tttcgatgat
      241 gtccattgat ttcatgatga cgccgcgctg atcgtcgagc ttgtgcatcg gcacctgatc
      301 ataactgtgc gcgttgagct cccagccgtt ttcgacacac gcctcgacga cttcagggta
      361 agtttcgcaa actctcgcgt tcaatgtgac cgtgggctta gccccaactt gccccagcat
      421 ctttcgcagt cgccagaagc ccacccgcat cccgtactca tgccagctcc agttcggcat
      481 atccgggagc tgaggctgac cttgcggagg tgaaatgacc atgcgcgcca ttggacgcga
      541 aatatcccac tcttcgagcg ccaacaccgg ccacaccact aaccggatgc cgctggggaa
      601 tcgaagcgcg gggcgtttgg taatcgcaga gaatgaaagt ctgtcacgcg gctgcatctg
      661 tcctgcgcac ccactgcgcc gttaagcaca ttatatcatg accacgatag aagcccgtgt
      721 ctctaccgaa gattagattc tgcaacagtg atcgaagccc gaggaatgct tgcgacgaga
      781 gcgtcattcg cgagccgcga gcacccgccg caacgtcgcg accagccgct cgaattcgat
      841 cggcttggtg tcgaactcgt cgcatccggc cgcaagcgcc ttctcgcgct cgccggcgag
      901 cgcgtgcgcg gagagcgcga tgatagggat gtcgcgtgtt tgtggatcgt ccttcagtcg
      961 tcgcgtcgcc tcccagccgt cgacgaccgg catttcgaga tccatcagga tgacgtcggg
     1021 gcgctcgctc agcgccatcg cgcagccctg ctcgccgtcc ggtgccgcca caacgtcgaa
     1081 atcgcccagc agctcgagcc gcatcttgag catgtagacg ttgtcgtcgt tgtcctcgac
     1141 gtagagtatt ttggtcacga cgctgcttcc atcccgttgt cgcgcgcgcc ggcatcgccg
     1201 gcttgcgatt tgatgtcctc gctcgaactt cttgcatgga cgccgccgcc gccgggcagg
     1261 cggacggtga agaccgagcc tttgcccggt tcgctcgcca ccgtcacgtc gccgcccatc
     1321 atgcgcgcga gcttgcgtgt gatggcaagg ccgagaccgg tgccgccgta gcgttgcgcg
     1381 gtcgatgcgt cggcctgggt gaactcctcg aacagcttgg cctgctgcgc ggccgtcatg
     1441 ccgatgccgg tatcggcgac cgcgaactcg acccagttgc ggccgtcggc gactttccgc
     1501 acccgcagct tcacctcgcc ctgcgtggtg aacttgcagg cattgctcaa cagattgagc
     1561 aggatctgcc gcagccgcat gggatcgacg gtgagcgccc cgagtttctc ctcggcttcg
     1621 accacgaggc ggttcttgtt ctgctcggcg agctggcgcg gggtgccgac gacctcgtcg
     1681 atgagcggtg ccaagctcac cgactcgggg ttgagctgga gcttgccggc ctcgatcttc
     1741 gagaggtcga gcacctggtt gatgaggccg aggaggtggg tgccggcgcg atgcacgcgc
     1801 ttgagcggct cggcggcttt ttcggtgccg aagcgcgccg cgttggtgac catcatctcg
     1861 gtcaagccga tgatggcgtt gagcggggtg cgcagctcgt ggctcatgct ggcgaggaac
     1921 tgcgacttgt gctggctcgc cagttcgagc tggcggctct tgtcctggat ttcgtcgaat
     1981 aggcgcacgt tctcgatggc gatcaccgcc tggtccgcga atgtcgaaac gagttcgatc
     2041 tgcttctcgg tgaacggacg cacggtcact ctcgccaaat tgaacacgcc catagggacg
     2101 ccttcccgca tcagcggtac tcccaggatg gtgcgaatgc ctccactgcg ctcgatcgcc
     2161 tcggaccagg caattcggga tcagtgcgga tgtcgggaat atgaacagtt cgcccctcaa
     2221 gcgccgtgcg cccgaccagc gtgttgcggc cgggttcgat taaaacctgt cccacgtact
     2281 ccacgaattc gggcgcaaat ccgaagttcg ccgcaaggcg gtagcccact ccttcccggc
     2341 gagcgatgaa gcccttatcc gcatcgcaca gacgggcggc cgacgcgacc agcgtttcga
     2401 gcaccgtaac aagatcgaac gtcgagcggc tgatgacctt gagcacgtcg gcagtggcgg
     2461 tctgctgctg cagcgattgc cgcagctcat tgagcagacg cgcgttttcc attgcgatga
     2521 ccgcctgcgc agcaaagttt tgcagcaatg cgatctgctt gtcattgaac ggccgcacct
     2581 cctgccggta caccatgaat gtgccgagca gcatctggtc cttgcgcaac ggcagccaca
     2641 gggcggtgcg agctccaccc aattcggcga gcgctcgctt gcctggatta ccggaccggt
     2701 agccctcatc gtcggtgatg tcggggatat gcacgaagct ctctccccga accagcgcat
     2761 aggatccggt cccgggaact gggtccaacg gcttgttgtg gaacgcggca aagagcgcgg
     2821 gaactcctcg catcgccact gcgtgcatgt gctccccgtc ataggtccac aagccaccga
     2881 aagctgcgtc ggacacgctc agcgcctttt ccaatatcgc gtcgaatacc gatgcaagct
     2941 caccagctga ggagttgata atctgaagca actcagcggt ggccgtctgc tgctgcagcg
     3001 actcgcgcag ctcgttgagc agccgggtgt tctcgatggc gatgacggcc tgggcagcga
     3061 agttcgtaac cagttcaatg tg
//
LOCUS       DATLJT010000962         1861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_751275,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000962 DATLJT010000000
VERSION     DATLJT010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751275"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VM910_22130"
     CDS             complement(<1..1371)
                     /locus_tag="VM910_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65255.1"
                     /translation="MRSLEQLARSPSRRAVLRSGLAGAFVLGFHLPARAVNEPEQPPD
                     STQGKFAPNAFIRIDRSGQTTLVMPQVEMGQGVYTAIPMILAEELDADFNTVTLEHAP
                     PNDKLYGNPIFGIQVTGNSNSIRSFWKPLRVAGATARLMLVQAAAQQWQVDPASCSTA
                     NGVVSHAPSGRTAGYGELIDAANTQPVPQDPPLKDPKNFTLIGKSLKRLDTPNKTDGK
                     VVYGIDAMLPGMKFATLAQCPVFGGKIAHVDDSAAKKVPGVQHVVVLDDLVAVVGDHM
                     WAAKKGLDALVITWKEGPNAKTNSSDIWNDLRAASKKDGVVAKSVGDIAKGLTQGERV
                     DAEYELPFLAHAAMEPLNCTVQVTPDSCEVWTGTQVLTRVQQAAAEAAGLPLEKVRAY
                     NHLLGGGFGRRLEADMVAAAVRIGKQVDGPVKVVWTREEDIQHDIYRPVYRDVISATL
                     SGGKIAA"
     gene            complement(1382..1840)
                     /locus_tag="VM910_22135"
     CDS             complement(1382..1840)
                     /locus_tag="VM910_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI65256.1"
                     /translation="MNLKINGEDQSFDAPNDMPLLWVLRDVLGMTGTKFGCGIAQCGA
                     CTVHIDGKPVRSCLLPVGAVGDRAVTTIEGVAATPSGAKVQKAWLELEVIQCGYCQSG
                     QIMSAAALLATTPNPDDSDIDAAMAGNICRCGTYVRIREAIKRAAGGGQT"
BASE COUNT          333 a          582 c          571 g          375 t
ORIGIN      
        1 ggcggcaatc ttgccgcccg acaaggttgc ggaaatcacg tcgcgataaa ccggccgata
       61 gatatcgtgc tggatgtctt cttcgcgggt ccagacgact ttcaccggcc cgtcgacctg
      121 tttgccgatg cgcacggcgg cggcgaccat gtcggcttcg agccgacgcc cgaagccccc
      181 gccgagaagg tggttgtacg ccctcacttt ctcgagcggt aggcccgcag cttccgcggc
      241 agcttgctgc acgcgggtga ggacctgggt gccggtccag acttcgcaac tatcgggcgt
      301 aacctggacc gtgcagttga gcggctccat cgccgcgtgc gccaaaaacg gcagctcgta
      361 ctcggcatcg accctttcac cctgggtcag gcccttggcg atatcgccga ccgattttgc
      421 gaccacgcca tcttttttac tggcggcgcg cagatcgttc caaatgtcgc ttgaatttgt
      481 ttttgcattc gggccctctt tccacgtgat gacaagcgca tcgagtccct ttttcgcggc
      541 ccacatgtga tcgccgacaa cggcaacgag gtcgtcgagt accacgacat gctggacgcc
      601 gggaaccttt ttggcggcgc tgtcatcgac gtgagcgatc ttgccgccga aaaccggaca
      661 ctgcgcgagc gtcgcaaact tcattcccgg cagcatcgcg tcgatgccgt agactacttt
      721 gccgtcggtc ttgttgggag tgtccaagcg cttaagcgac ttgccgatca gcgtgaaatt
      781 cttcgggtcc ttgagcggtg gatcttgcgg gaccggctgg gtattcgcgg cgtcgatgag
      841 ctcgccgtaa ccggcagtgc gtccgctcgg tgcgtgactg acaacgccgt tagcggtcga
      901 gcaactggcc ggatcgactt gccattgttg tgccgccgcc tgaacaagca tcagccgtgc
      961 cgtggctccc gccacgcgta acggcttcca gaacgaacga atggaattgg agtttccggt
     1021 gacctgaatc ccgaagatcg gattaccgta gagcttgtcg ttgggcggcg cgtgctccag
     1081 cgtcactgtg ttgaagtcgg catcgagttc ctcggcaaga atcatcggga tcgccgtgta
     1141 gacgccctgc cccatttcga cttgcggcat caccagcgtc gtctgacccg agcggtcaat
     1201 gcggatgaaa gcattaggcg caaacttgcc ctgcgtgctg tccggcggct gttccggctc
     1261 attgacggca cgcgccggga gatgaaagcc gagcacgaag gctcccgcca gaccgctgcg
     1321 caggaccgcg cgccgcgatg gcgaacgagc tagctgttcg agggagcgca tcggtagcct
     1381 cctacgtctg accgccgccg gcggcgcgct tgatcgcttc gcggatgcgg acataggtgc
     1441 cacagcggca gatattgccc gccatcgcag cgtcgatatc ggaatcgtca ggatttggcg
     1501 tggtagcgag caacgccgcc gccgacatga tctgaccaga ctggcaatag ccgcactgga
     1561 tgacttccag ctccagccag gccttctgca ccttggcccc gctgggagtg gcggcaactc
     1621 cctcgatcgt ggtgacggcg cggtcgccca cggcaccgac cggaagcaga caagaccgca
     1681 caggtttgcc gtcgatatgc accgtgcagg cgccgcattg cgcgatgccg cagccgaatt
     1741 tggtgccggt cataccgagc acgtcgcgca gcacccaaag cagcggcatg tcattgggcg
     1801 cgtcgaagct ttggtcttca ccgttgattt ttaaattcat gatgacgaac ctccgccgtt
     1861 g
//
LOCUS       DATLJT010000963         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_64538,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000963 DATLJT010000000
VERSION     DATLJT010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64538"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VM910_22140"
     CDS             <1..190
                     /locus_tag="VM910_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVI65257.1"
                     /translation="RELQVLKGLLAGLPNKSIAYDLEISPRTVEIHRARVMDKMGARS
                     LSELVRLALAAGVQSGSQ"
     gene            complement(279..587)
                     /locus_tag="VM910_22145"
     CDS             complement(279..587)
                     /locus_tag="VM910_22145"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65258.1"
                     /translation="MPLLVKAERVILAGAEEDDPSLTDGLADLSRQLAWHGVSVDVEF
                     MSSTAGPASKVLMEAARSHQAGLLIMGGYGHSHTREIVFGGFTQSVLESAEMTVFLIH
                     "
     gene            complement(1168..>1517)
                     /locus_tag="VM910_22150"
     CDS             complement(1168..>1517)
                     /locus_tag="VM910_22150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65259.1"
                     /translation="KSTPEAARAVTVAMPFLSIAKQIVIMTVAEDETTIEEEGAARLM
                     ANLRWDGFSASVRRLEPGAQGGAETLLAAAREQAALLVMGGYGHSRLREWIFGGFTER
                     ILRAAEIPVLIAH"
BASE COUNT          299 a          487 c          448 g          283 t
ORIGIN      
        1 gcgcgagctc caggtgttga aaggtctcct cgccgggctg ccgaacaaat cgattgccta
       61 cgatttggag attagcccac gcaccgtcga gatccaccgc gcgcgggtca tggacaagat
      121 gggggcgcgc agtctctccg aactggtccg gcttgccttg gccgccggcg ttcagtcagg
      181 gtctcagtga cgacatcgga gctgacgctg tctctgggga gatccgttcc tgctccggac
      241 gaggtcagcc gacggcttcg gcactagggc acgttacatc agtggatcag aaaaactgtc
      301 atctcggcgg attcgagaac agattgggta aagccaccga aaacaatttc acgcgtgtgg
      361 ctatggccgt agccgcccat aatcaacagc cctgcttgat gggaacgggc ggcctccata
      421 agcactttgc tcgcggggcc agcagtcgac gacatgaact cgacatcgac gctgacgccg
      481 tgccaggcta gctggcggga aaggtcagcc aatccgtccg tcagcgacgg gtcgtcttct
      541 tcggcgccgg cgagaatgac gcgctccgct ttgaccagga gcggcatcgc ggcaccgacc
      601 gcatgcgccg cctccgccgt ttccttccag caaaccacga ccgtgccgag aagcgggtgc
      661 gagagtcgcg gaggggcaat cagcaagggt ctgccgcaac caagcagcag cctttcgagc
      721 agatcgggcg ataagccatt cggccccgtc gggcgtgcta cgacaatcaa gtcgtaatgc
      781 ctacacaaaa catcaaacga cgatcggcct cgcccatctc ctcgcgccag cttgcagaaa
      841 ccccaccgcc ggatcggggc tggtccgtga tgctaattcc gtgacgctcg cagaatcgcg
      901 cgaaattatc tctcgcaacg gcggtgcgca tgacgcactc ggtctccagg cgctgcatca
      961 ttttccacat cgccggtccc ctcgcgaact cggcatgcgg ttgccatatt gccgccgccg
     1021 ggatcgactt gcacatgaaa gaattcgagg tgcgcatcca agggctgtgc gatgccaaaa
     1081 gctgcggcga agacggcgtg gtcgctgccc ccgccaccag ccaacacgag tatgtttttc
     1141 atgctggttt gctccgacaa gtcgtcctca atgtgctatc agaaccggta tttccgccgc
     1201 gcgcagaatc cgttcggtga agccaccgaa aatccactcg cgcagccggc tatggccgta
     1261 gccgcccatc accaataggg cagcttgttc gcgagccgcc gcgagcagtg tttcggctcc
     1321 tccctgcgcg ccgggctcta ggcgacgcac cgatgccgaa aacccgtccc agcggagatt
     1381 ggccatcagg cgggcagctc cttcttcctc aatcgtggtc tcatcttccg cgacggtcat
     1441 gatgacgatc tgctttgcga tcgacaggaa cggcattgcc acggtaacgg cgcgtgcggc
     1501 ttcgggtgtc gacttcc
//
LOCUS       DATLJT010000964         3241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_751326,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000964 DATLJT010000000
VERSION     DATLJT010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751326"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..523
                     /locus_tag="VM910_22155"
     CDS             164..523
                     /locus_tag="VM910_22155"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVI65260.1"
                     /translation="MTEQWRASSARGLRVACREEPEVLIVECHGQLTYGNSQTLKSEV
                     KVRIPGHKRLVLDLKGVPQIDSSGLGTVMALYVSARARGCKLELVNANDQIRALFSVT
                     NLLSLFEAAGRHHGKTI"
     gene            complement(535..1356)
                     /locus_tag="VM910_22160"
     CDS             complement(535..1356)
                     /locus_tag="VM910_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVI65261.1"
                     /translation="MLTQTDKGHVFQALHKSNGAFIIPNPWDVGSARLLAHMGFKALA
                     STSMGYAFSIGKCDNAVDRGSIIAHIAAIASASDLPVSADLENGFGDGPAVVAETIRL
                     AAAAGVVGGSIEDASGRADHPIYEKEHAAERIRAAAEAARSLPFTFTLTGRAENYLHG
                     RPDLRDTISRLQAYQEAGADVLYAPGLATREDIAAVVGSVDRPVNALMGLQGVLLSLA
                     ELSALGVKRVSVGSSLCRAALGAFLRAAREMREHGTFTFANEAVSSREITEIFRA"
     gene            complement(1377..2393)
                     /locus_tag="VM910_22165"
     CDS             complement(1377..2393)
                     /locus_tag="VM910_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HVI65262.1"
                     /translation="MAAQKNVRAVITRPGGPEVLKLATEDLPSPAPGQVRVKVLAAGV
                     AYADVLMRRGLYPGVPRPPFTPGYDIVGEVDALGQGVTGFALGLRVAALTMVGGYSRF
                     ALVPAAHLVPVSQQLDPAEAVSLVLNYVTAYQMLHRIAALHSGQGLLVHSAAGGVGTA
                     ALQLGRLSGLRLFGTASKAKHEILFSLGATPIDYRNESFVARIRQLVPQGLDSVFDPI
                     GGRHWWESYRCLRPGGSLVCYGAQAAVTEGKFAAGIGFALLGLMKMIPDRRRASWYNV
                     KVLHDAHPEWFREDLTKLFELLAERQIQPLIASRLPLRDAPRANEMLEKAQVSGKIVL
                     LPHS"
     gene            complement(2507..>3241)
                     /locus_tag="VM910_22170"
     CDS             complement(2507..>3241)
                     /locus_tag="VM910_22170"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVI65263.1"
                     /translation="RLIVGTGKYRSFQEMARAHAACGAEMVTVAVRRVNLTDKSKESL
                     LDYIDREKIFILPNTAACYSADEAVRTARLAREVGLSNWIKLEVIGDQQTLFPDTEQL
                     VEATKVLVKDGFVVLPYTNDDLIAARKLIDAGASAVMPLAAPIGSGLGIQNPANLRIL
                     RERITEVPMIVDAGVGTASDAAIAMELGADGVLMNTAIAEAADAVLMAEAMRGAVIAG
                     HSAYLAGRMPKRLYASASSPLTGVVR"
BASE COUNT          689 a          969 c         1020 g          563 t
ORIGIN      
        1 acaagatcgg cgagcaagcc gagcgcgcag aacgtcatcc agaagacctt gcgctgcatc
       61 accgcagtct aacccgtttt ttcccggtct ggtcgtggcg cagagtggaa tttgttcggc
      121 aagtgcgata aaaggtcaga attcccagcg atcggaggcg gccatgacgg agcaatggcg
      181 ggctagctcg gcacgaggac ttcgggtggc gtgccgggaa gaaccggagg tactgatcgt
      241 cgagtgccac ggccagctga cctatggcaa ctcgcaaacg ctaaagagcg aagtaaaggt
      301 caggattcct ggacataagc ggctggtgct cgacttgaag ggtgtgcctc agatagacag
      361 ttcggggctc ggaacggtga tggcgctcta cgtgtcggcc cgggcccgag gatgcaagct
      421 cgaactggtg aatgcaaacg accagatccg tgccctgttc agcgtcacca atttgctgtc
      481 gttgttcgaa gccgcagggc ggcaccacgg aaaaacaatc tgagcgtgcg gacttcaagc
      541 tcgaaagatc tccgtaatct cgcgcgaact caccgcttca tttgcaaatg tgaaggtgcc
      601 gtgctcgcgc atttcgcgcg cagcccgcag aaatgcaccg agcgccgcgc gacacagaga
      661 actgcccacg ctgacccgtt tcacgccaag cgccgacagc tcggcaagac tcagcaggac
      721 accctggagc cccataagag cgtttacagg gcgatcgacc gaaccgacga cggcagctat
      781 atcctcccgg gtggcaaggc ccggagcata gaggacatcg gcgccggcct cctgataggc
      841 ctgaagacgc gagatcgtgt ctctcaaatc aggacggccg tggaggtagt tttccgcgcg
      901 accggtcagc gtgaacgtaa acgggaggga gcgcgcggct tcggctgccg cgcggattct
      961 ttcggccgca tgctcctttt cgtagatcgg atgatcggca cgaccactgg catcctcgat
     1021 cgaaccgccg acaacaccgg cggcggcggc aagccggatc gtttctgcta caactgccgg
     1081 cccatccccg aagccattct cgagatcggc gctgaccggc agatcggagg cggaggcaat
     1141 cgcagcgata tgcgcgatga tggagccgcg gtcaaccgcg ttatcacact tcccgatcga
     1201 gaacgcatac cccatgctcg tcgaggccag cgctttgaac cccatgtggg ccagcagacg
     1261 agcgctgccg acgtcccatg gattggggat gatgaaagct ccattgctct tgtggagcgc
     1321 ttgaaagaca tgccctttgt cagtttgcgt caacatggat ctcacgacct cgcgagtcag
     1381 gaatggggca gcaaaacaat ctttcccgag acttgcgcct tctcgagcat ttcatttgcg
     1441 cgcggcgcat cgcgcagggg aagccggctg gcaatgagcg gctgaatctg ccgctcggcg
     1501 agcagctcga aaagcttcgt cagatcctcg cggaaccact cgggatgggc gtcgtgcagc
     1561 accttgacgt tgtaccaaga agcacgccgt ctgtcgggga tcattttcat caatcccagc
     1621 aaagcgaagc cgattcctgc ggcaaacttc ccttctgtga ccgccgcctg agccccgtag
     1681 cacacaagcg agccaccggg gcgaaggcag cggtaggact cccaccagtg tcttcccccg
     1741 atgggatcga agacggaatc caagccttgc ggcacaagct ggcggatccg ggcgacgaag
     1801 ctttcgttcc tgtagtcaat cggagtggcg ccaagcgaga agaggatttc atgcttagcc
     1861 ttcgaagcgg tgccgaaaag gcgcaggccc gagagcctgc cgagctgaag cgcggcagtg
     1921 ccgacgccgc ctgcggcact gtgcacaagc aagccctggc ctgagtgcaa cgcggcaata
     1981 cgatgcagca tttgatatgc agtaacgtag ttcagaacga gactcacggc ctctgcgggg
     2041 tcgagttgct gcgaaacggg taccaaatgt gccgctggca ccaaggcgaa gcggctgtac
     2101 ccaccaacca tggtgagggc cgcaacgcgc aggccgagcg cgaagccggt aacgccttgg
     2161 ccgagcgcgt ccacctcgcc aacgatgtcg tagccgggcg taaaaggggg gcgtggcacc
     2221 ccggggtaaa gcccgcgacg catcaggacg tcggcgtacg cgacaccggc agcaaggacc
     2281 ttcactcgaa cctgacccgg ggcgggagaa ggcaagtcct cagtcgccaa tttgagaacc
     2341 tcggggccac ccggtcgggt gatcaccgcg cgtacgtttt tctgggcggc catcggagcc
     2401 aaagccttgc gcgggcgagg taacgagcaa ggcccagggt atccgatgcg ggggccgttt
     2461 cgaagggcta ttttccggga ggccgagaca aagagttaaa gcctcctcag cgcaccacac
     2521 cggtcaatgg cgaagaggcg gaggcgtaca ggcgcttggg catccggccg gcgagatagg
     2581 cgctgtgacc ggcgatcacg gctccgcgca tggcttccgc catcaggaca gcatcggcgg
     2641 cttcggcaat cgccgtgttc atcagcacac catcggcgcc aagttccatg gcgatcgcag
     2701 cgtcagacgc cgtgccgaca ccagcgtcca caatcatcgg cacttccgtg atgcgctcgc
     2761 gaagaatgcg cagattggcg ggattctgga tgccgagacc cgagccaatc ggtgctgcca
     2821 gcggcatgac ggctgaagcg cccgcgtcaa tcagcttgcg agcggcgatg aggtcatcgt
     2881 tggtgtaggg cagaacgaca aaaccatcct tcacgagtac cttggtggct tcgacaagct
     2941 gctccgtgtc ggggaagagc gtttgctggt ccccgataac ttcgagcttg atccaattcg
     3001 agaggccaac ctcgcgggcc agccgcgccg tgcggacggc ttcgtccgcg gaatagcaag
     3061 cagcggtgtt cggcaggatg aatattttct cgcgatcaat gtagtcgagc agtgattctt
     3121 tggacttgtc cgtcagatta acgcggcgaa cggccaccgt gaccatttcc gcaccgcatg
     3181 cggcgtgggc gcgtgccatt tcctgaaagc tgcggtactt ccctgttcca acgatgaggc
     3241 g
//
LOCUS       DATLJT010000965        10582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1673314,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000965 DATLJT010000000
VERSION     DATLJT010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673314"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="tatC"
                     /locus_tag="VM910_22175"
     CDS             complement(<1..663)
                     /gene="tatC"
                     /locus_tag="VM910_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVI65264.1"
                     /translation="MTDADIEASKAPLLDHLIELRARLIKALLGFGIAFIFCFFFAKQ
                     IYNVLVWPFVWVAGAENSKFIYTALLEYFITQLKLALFGAGFISFPIVATQIYKFVAP
                     GLYRHERQAFLPYLIATPVFFLLGSLLVYFVVFPMLIRFSLGMQQMGGGGSAQIELLP
                     KVGEYLSLMMSLIFAFGIAFQLPVILTLLGRVGIITSKMLREKRRYFIVIAFVIAAVL
                     TPP"
     gene            complement(660..1121)
                     /gene="tatB"
                     /locus_tag="VM910_22180"
     CDS             complement(660..1121)
                     /gene="tatB"
                     /locus_tag="VM910_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HVI65265.1"
                     /translation="MFDIGWSEFVLIAVVALIAIGPKELPGVLRTVGQWMTKARRMAA
                     EFQSQFQEAMREAEMADLKKSFDEVREAASGFGSGNIMTSLEKDVSNALRIDDVDKPK
                     DAIAPPAAATPEAPAVDSSTTEPLAITADAPSPPQDNAADAPADLKDAKAS"
     gene            complement(1202..1438)
                     /locus_tag="VM910_22185"
     CDS             complement(1202..1438)
                     /locus_tag="VM910_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVI65266.1"
                     /translation="MGSLSIWHWIVVIAVVLLLFGRGKISDLMGDVAQGIKAFKKGMR
                     DEETASDKPEPVKSIDHNTASSSTTRSDVGSKAV"
     gene            complement(1691..2407)
                     /gene="scpB"
                     /locus_tag="VM910_22190"
     CDS             complement(1691..2407)
                     /gene="scpB"
                     /locus_tag="VM910_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVI65267.1"
                     /translation="MASLAEKRMDDAEDAPVDPQARPEELRLLEALLFASAEPLDQAT
                     LAKRMPDGVDIKAALVRLQEEYASRGVNLVRIGNKWTFRTAGDLAWLMTRESTETRRL
                     SRAAIEMLAIIAYHQPVTRAEIEEIRGVVTSKGTLDVLLETGWIRPRGRRKTPGRPLT
                     FGTTEAFLSQFSLETLSDLPGLEELKGTGLLDSRLPTGFSVPTPSDDPTLREDEDPLE
                     AGDLELMLVPPVEPEGGSGG"
     gene            complement(2429..3250)
                     /locus_tag="VM910_22195"
     CDS             complement(2429..3250)
                     /locus_tag="VM910_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HVI65268.1"
                     /translation="MTAEILSFETGRPAELADGEPSLVVDVEGYEGPLDLLLALARQQ
                     KVDLSKISILALADQYLQFIEAARKVRLELAADYLVMAAWLAFLKSRLLLPEPPTAEG
                     PSAEEMATALANRLRRLEAIREASNRLMNRPQLQRDIFPRGNPETIAEIRHQKYTATL
                     FDLLTAYAVQRQQRVLANVHLTRRTVWSLAEARASLERLIGLAEDWSCLDEYLISYVV
                     EPSQRATVFASSFAAALELVREGEMELNQKEAFAPLYFRKRPPQGDGAPATASDL"
     gene            complement(3247..4275)
                     /gene="nagZ"
                     /locus_tag="VM910_22200"
     CDS             complement(3247..4275)
                     /gene="nagZ"
                     /locus_tag="VM910_22200"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HVI65269.1"
                     /translation="MTTKAFITGVAGTNLSAEERNFIAAERPWGFILFKRNVADPAQV
                     TALTDQLRAAVGEPNAPVLIDQEGGRVQRLSPPHWPLYPPGAAFGSLYDIDAELGLRA
                     ALLSARLIGDDLAELGVTVDCLPLADVPVAGADAVIGNRAYGTEPTKVSAIARAVTVG
                     LAQGGILPVLKHIPGHGRATADTHLRLPVVDTAQAELEKTDFAAFRPLVDLPMAMTAH
                     VVFSAYDPAHPATTSATMIETVIRGLIGFQGLLMSDDVSMNALAGSIAERTRAIFAAG
                     CDLVLHCNGKLDEMREVASETPELSGKALIRAERALASRKPPVPFDRAAARAELDALI
                     DRLGAAEA"
     gene            complement(4324..5829)
                     /locus_tag="VM910_22205"
     CDS             complement(4324..5829)
                     /locus_tag="VM910_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVI65270.1"
                     /translation="MAHRYQNRPFPADDYNRGGDQPGRGESDPLAELARLIGQTDPFG
                     NQGRANPPPPPRANPPERHQPQHEPLPAEEEEDAPHAGPPPWMQRANARVVQRETPRE
                     ALREFPDDIPPEEDYPAPVYPRHRYAQPAPPEEQYVEEPGYDEAEEHPDPSRYDDALY
                     GQIENGAQDYQRDPAYPNDPYAFQDGDDEEAEQHSNKRRGVLLKLVAVIALAVVGAGS
                     ALAYRTYVGSTRAGEPPIIKADNSPTKIVPAPADNTAKAPDRMPSGDGTEKIVSREEA
                     PVDINAKSGGARVVFPPLSPNASPPAPASVSTSTIPIPPPSTTSSPTPSNGTLPNNEP
                     RKIRTVAVRGDQADSAAVPVGAPPAAPSATATKSGPAPKTATARGAAPSGNAPLSLAP
                     DQAPAPAARVATTTAPPTPAPNANAASGGYLVQVSSQRSETDAQASFKALQSKFPSVL
                     GSQTPVIKRADLGDKGIYYRAMIGPFGTPEEASQFCGNLKNAGGQCVVQRN"
     gene            complement(5933..7729)
                     /gene="argS"
                     /locus_tag="VM910_22210"
     CDS             complement(5933..7729)
                     /gene="argS"
                     /locus_tag="VM910_22210"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HVI65271.1"
                     /translation="MAEKSSSLHLFADVLARVHAICASLATEGRWPDGIDFSRIVVEP
                     PRDASHGDMATNAAMVLAKEAKSKPRDLAEAIAARLGADELIAKVDVAGPGFINLTLK
                     PKAWAEALLTVLGEGDDYGRIAPDASPRVNVEYVSANPTGPMHVGHCRGAVFGDALAS
                     LLQFAGRDVTREYYINDAGAQVDVLARSAYLRYREALGEGIGAIPDGLYPGDYLKPVG
                     QALASEYGDKLHAMSEAEWLPIVRAKAIAMMMDEIKDDLAALNIRHDVFFSERSLIEG
                     GNNKVAETIDYLRSRGDVYEGRLPPPKGTPVEDWEDREQLLFRATAFGDDVDRPLIKS
                     DGTYTYFASDIAYHKNKFDRGFRDLVDVWGADHGGYIKRVQSAVKAVTGEEAALDVKI
                     VQLVRLLRAGEPVKMSKRAGDFVTLREVVDEVGKDAVRFMMLYRKNDAVLDFDLAKVI
                     EQSRDNPVFYVQYGHARGHSIFRNAREEVLPDLPVGTEARLAYLGTAPVERLTDPAEI
                     GLLKQLALYPRTIEGAAAAHEPHRIAFYLYDLASEFHALWNKGHDLPNLRFIINNDAG
                     ITKARLAMVQGVVSVLSSGLGILGVHAPEQMR"
     gene            complement(7740..8948)
                     /locus_tag="VM910_22215"
     CDS             complement(7740..8948)
                     /locus_tag="VM910_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HVI65272.1"
                     /translation="MSVGMAAPRAPYACDPDRSRGRQFVEPPSKTRSPFRRDCDRVIH
                     STAFRRLKHKTQVFVFHEGDHYRTRLTHSLEVAQIARALARQLGLDEDITEALALAHD
                     LGHPPFGHAGERALDACLKAHGGFDHNAQTLRVVTMLEHRYPNFDGLNLTWESLEGIV
                     KHNGPLIDASGAALGHYREAGIPVGIAQFDAIYNLELSGFASLEAQVAAIADDIAYDA
                     HDIDDGLRAGLFAVDDLKVMPLTAEMVADIDSRYPGLDQDRRGAELVRELISRLIGAV
                     TSESGRRIAIVQPKSVDDVRHCGHQLIAFSPDIADAEAGIKAFLRQRMYRHPRVMRVM
                     AEAEQIVLDLFARYQRIPGDLPPEWLEGAEREGESARARRIGNFIAGMTDRFALMEHQ
                     RLFDLTPELR"
     gene            9061..9390
                     /gene="erpA"
                     /locus_tag="VM910_22220"
     CDS             9061..9390
                     /gene="erpA"
                     /locus_tag="VM910_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HVI65273.1"
                     /translation="MTTAVTISERAARRIGEILTREGEGAMLRISVEGGGCSGFQYKF
                     DVEHAKAEDDLVIERDHAVVLIDPASVPFLAGSEVDFVDDLIGASFRVINPNATASCG
                     CGTSFSI"
     gene            9403..10194
                     /gene="xth"
                     /locus_tag="VM910_22225"
     CDS             9403..10194
                     /gene="xth"
                     /locus_tag="VM910_22225"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVI65274.1"
                     /translation="MRIATWNVNSIRQRIEPVMSWLKETSPDIACLQEIKCTDDAFPR
                     LEFESLGYNVVTHGQKTFNGVALLSKLPFDETKSGLAGDDEDAHARFLEGVVTLKSGV
                     LRVACLYLPNGNPPDTDKYPYKLNWMSRLFEYSKQRLKAEEPLILAGDFNVIPTAEDV
                     YNPPAWVNDALFRTTTRESFQALLGLGLTDALRAVNDAAGQYTFWDYQAGAWQKNWGL
                     RIDHLLLSPQASDRLIDVGIDSYVRAWEKPSDHVPVWADLDLEAR"
     gene            complement(10211..>10582)
                     /locus_tag="VM910_22230"
     CDS             complement(10211..>10582)
                     /locus_tag="VM910_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HVI65275.1"
                     /translation="EMFYYAASYFGNADAQYDLARLYLKTANATPSEFTYGARWLGLA
                     AQKGQHQAQAMLGQMLFNGDRIPPQRARGLMWLTLACENAGPDESWIKESYNRAIASA
                     SDNDRAMALQMLEHWVQGRRD"
BASE COUNT         1938 a         3319 c         3295 g         2030 t
ORIGIN      
        1 cggaggggtc agcacagcag cgatgacgaa ggcaatgacg atgaagtagc ggcgcttctc
       61 gcgcagcatc ttcgacgtga tgatgccgac acgcccgagc agggtcagga tcaccggaag
      121 ctggaacgcg atgccgaagg cgaagatcag cgacatcatt agcgacagat attcgcccac
      181 cttaggcaac agctcgatct gcgcgctgcc tccgccgccc atttgctgca tccccaagga
      241 gaagcggatc agcatcggaa agacgacgaa atagaccaac agcgatccta gcaggaagaa
      301 gaccggggta gcgatcaggt acgggaggaa cgcctggcgc tcgtgccggt aaagtcccgg
      361 cgcgacgaac ttgtagatct gggttgccac gatcggaaac gagatgaagc cggcgccgaa
      421 cagcgcaagc ttcaattgcg taatgaaata ttccagaagt gctgtgtaaa tgaacttcga
      481 gttctcagcg cccgccaccc agacaaaggg ccagaccagc acgttgtaga tctgcttggc
      541 gaagaagaag cagaagatga aggcgatgcc gaagccgagc agcgccttga tcaggcgcgc
      601 gcgcaactcg atcagatgat caagaagcgg cgccttgctg gcctcgatat cggcatcggt
      661 catgacgctt tggcgtcctt caaatctgcc ggcgcgtctg ctgcattatc ctgcggcggc
      721 gacggcgcat cggcggtaat cgctaacggc tcagtggtgc tgctgtcgac ggccggcgct
      781 tccggggtag ctgcggcagg cggcgcaatc gcatccttcg gcttgtcgac gtcatcgatt
      841 ctgagcgcgt tggaaacgtc cttctccagc gaggtcatga tgttgccact gccgaaacca
      901 ctggcggctt ccctgacctc gtcgaagctt ttcttgaggt cggccatctc ggcctcgcgc
      961 atcgcctcct ggaattggct ctggaattcg gcggccatcc tgcgcgcctt ggtcatccat
     1021 tgtccgaccg tcctcagtac gcccggcaat tccttcgggc cgatggcgat cagcgcgaca
     1081 accgcgatga ggacaaattc actccacccg atgtcgaaca taaaaaatac cgctcacgcg
     1141 aggccgtgtt gccccatcgc tttggtgttg gggctgctcg cgtctccttc cggcgttcgg
     1201 ttcagacggc cttgctgccg acatccgacc gtgtcgtcga agacgacgcg gtgttgtgat
     1261 cgatggactt cacgggctcc ggtttgtcgg acgcagtctc ctcgtctcgc atgcccttct
     1321 tgaaggcttt gatgccttgg gcaacgtcgc ccatcagatc ggaaatcttg ccgcggccaa
     1381 acagcagcag gacgactgcg atcaccacga tccagtgcca aatgctaagc gaacccatcc
     1441 tgcaaccctc caactagagc acgatccgga aaattgggta ccggttttcc ggataattca
     1501 tgctcaaaca acaacctcag cgcgacggcg aattgaccgg gtcgaccgag atttggccgg
     1561 cgatccggcc caattcttta gcggaaagta ggcctccggg gtgtcaaaaa caaggaccca
     1621 tgcgccagca aatcgctgtc gagcctacgc attattgcta ccgttaatcg gcccgatgca
     1681 cggtccggaa ctagcctccg gaaccgcctt ccggctcgac cggcggcacc agcatgagct
     1741 ccagatcgcc agcttcgagc gggtcctcgt cttcgcgcag cgtcggatcg tccgagggcg
     1801 tcggcacact gaaaccggtc ggcaggcgcg agtccaacag ccctgtccct ttcagttcct
     1861 cgagcccggg caggtcgctg agcgtttcca gactgaactg cgacaggaaa gcttcagtgg
     1921 ttccgaaagt cagcggacgg cccggtgtct tgcggcgacc gcgcggcctg atccatccgg
     1981 tctccaggag cacatcgagc gtccccttgg aggtgaccac gccgcggatc tcctcgatct
     2041 cggcgcgggt cacgggctgg tgataggcga tgatcgccag catctcaatc gcggcgcgcg
     2101 acaagcggcg cgtctccgtg ctttcccgcg tcatcagcca tgccaaatcg ccggcggtgc
     2161 ggaacgtcca cttgtttccg atgcgaacca ggttgacccc gcgcgatgcg tactcctcct
     2221 gcaagcgcac gagcgcagcc ttgatgtcga cgccatcggg catgcgcttg gcaagcgttg
     2281 cctggtcgag cggctcggcg gaagcgaaca gcagcgcttc caggagcctg agctcctcag
     2341 gccgcgcttg cggatcgacg ggcgcgtctt cggcgtcgtc catgcgtttc tccgccaggc
     2401 ttgccatggc acggtctcct tcttgcactt acaaatctga cgccgtcgcc ggcgcgccat
     2461 cgccttgcgg tggacgcttg cggaaataga gcggcgcgaa ggcctctttc tgattcagct
     2521 ccatctcgcc ctcgcgcacg agttcgagtg ccgcagcaaa gctcgatgca aatacggttg
     2581 cgcgctgcga aggctcgacg acatagctga tgagatattc gtcgaggcaa ctccagtctt
     2641 cggcgagccc gatgaggcgc tcgagcgaag cacgcgcttc cgcgagcgac cacaccgtgc
     2701 gcctggtaag atgcacgttt gcgagcacgc gctgctggcg ctgtaccgca tatgcggtaa
     2761 gcaggtcgaa tagcgttgcc gtgtattttt ggtgccggat ctcggcgatg gtttccggat
     2821 tcccgcgggg aaaaatgtcg cgctgcaatt ggggccggtt catcaagcgg ttggacgctt
     2881 cgcggatggc ctccagccga cgcagccggt tggcgagtgc ggtcgccatt tcctccgcgc
     2941 tcggtccttc agcggtcggc ggttcgggca gcaacagccg cgatttcagg aaggcgagcc
     3001 atgccgccat gacgagataa tcggcggcaa gttcgaggcg gacctttcgc gcagcttcga
     3061 tgaactgcag atactgatcg gcaagcgcga ggatcgagat cttggaaaga tcgaccttct
     3121 gctgccgcgc cagcgcgagc aacagatcga gcggaccttc atagccctcg acatcgacga
     3181 ccagagacgg ctcgccgtcg gcgagttccg ccggcctgcc ggtctcgaag gacaaaatct
     3241 ccgcggtcat gcctctgccg ctcccaatcg atcgatcaac gcatccagct ctgctcgcgc
     3301 cgcggcccgg tcgaagggca ccggcggctt gcgcgacgca agagcgcgct ctgctcgtat
     3361 caacgcttta ccggacaatt cgggcgtctc gctggcgact tcgcgcatct cgtccagctt
     3421 gccgttgcag tgcagaacca gatcgcagcc cgcagcaaaa atggcccgcg tgcgctcggc
     3481 aatcgatccc gccagcgcgt tcatggacac atcatcactc atcagcaaac cttggaaccc
     3541 gatcaagccg cgaatcaccg tttctatcat tgtcgcagat gtcgttgcgg gatgggcagg
     3601 gtcataggcg ctgaacacaa catgtgcagt catcgccatg ggtaggtcga caagcggccg
     3661 gaaggcggca aaatcggtct tttccagctc tgcttgcgcg gtatcgacca ccggaaggcg
     3721 aagatgcgtg tcggcggtcg cccgtccatg gccgggaata tgcttgagga ccggtaaaat
     3781 gccaccttga gccagcccga ccgtcaccgc ccgtgcgatc gctgaaacct tggtgggttc
     3841 ggtgccatag gcgcggttgc cgattacagc gtccgcgcct gccacgggca catcggccag
     3901 cgggaggcag tcgaccgtga cgccaagctc ggcgagatcg tcgccaatca gccgcgcgct
     3961 cagcaaagcc gccctgagcc ccagctccgc atcgatgtcg tagaggctcc cgaaggccgc
     4021 tccgggcgga tagagcggcc agtgcggcgg gctgagccgc tgcacccgtc cgccttcctg
     4081 atcgatcaga accggagcat ttggctcgcc taccgcagca cgcagttggt cggtcagcgc
     4141 agtgacctga gcagggtccg cgacattccg cttgaaaaga atgaaccccc acggccgctc
     4201 cgcggcgatg aaattgcgct cctcggcgct gaggttcgtt cccgcgacgc ccgtgataaa
     4261 ggcttttgtg gtcatggcgg gccgattagc ccgcggccaa gcagggggtc aaggaaaccg
     4321 ccgctaattc ctctggacca cacactgacc gcctgcattc ttgaggttgc cgcaaaactg
     4381 cgaagcttcc tcaggcgtgc cgaagggccc gatcatggcg cgataataaa tccccttgtc
     4441 gccgagatcg gcgcgcttga tgacaggcgt ctgcgatccc aggactgagg ggaacttgct
     4501 ctgcagtgcc ttgaacgagg cctgggcgtc ggtttcgctg cgctgcgagg agacctggac
     4561 gaggtagcct ccgcttgccg catttgcgtt gggagccggc gttggcggcg ctgtggtcgt
     4621 agctactcgc gctgcgggcg ccggcgcctg atcgggtgcc agcgagagag gcgcgttgcc
     4681 tgatggcgcc gcgcctcgtg ctgtggcggt cttgggagcc ggcccggatt ttgtagccgt
     4741 cgctgatggg gcagccggcg gggctccgac cggcaccgcg gcgctgtcgg cctggtcgcc
     4801 ccggaccgcg accgtccgga ttttgcgcgg ctcgttgttg ggcagcgtgc cgttggacgg
     4861 ggtcgggctg ctggtcgtgc tcggcggcgg tatgggtatg gtgctggtcg agacactcgc
     4921 cggcgccggc ggactggcat tcgggctcag cggcggaaac acgacgcgcg ctccgccgct
     4981 cttggcattg atatcgaccg gagcttcctc gcgggacacg atcttctcag tgccgtcgcc
     5041 cgacggcata cgatcgggcg ccttcgccgt gttgtcagca ggtgcgggta cgatctttgt
     5101 tgggctgttg tctgccttga tgatcggcgg ctcgccggcc cgcgtcgatc cgacataggt
     5161 gcggtaagcc agcgcggagc ccgcccccac gacggcgaga gcgataacgg cgaccagttt
     5221 gagcaacacg ccgcgacgct tgttgctgtg ctgctcggcc tcctcgtcgt cgccgtcctg
     5281 gaaggcataa gggtcgttcg gataggccgg gtcgcgttga taatcctgtg caccgttctc
     5341 gatttgccca tacagcgcgt catcatagcg cgagggatcg ggatgctcct cggcctcgtc
     5401 gtagccgggc tcctccacat actgctcctc gggcggagcc ggttgcgcgt agcgatgccg
     5461 cggatacacc ggggccgggt aatcctcttc tggcggaatg tcgtcgggga actccctcag
     5521 cgcctctctt ggcgtttccc tctggacgac tcgcgcgttg gcgcgctgca tccacggcgg
     5581 cggacccgcg tgcggagcgt cctcctcttc ctccgctggt agcggctcgt gctggggttg
     5641 atgccgttca ggcggattgg cgcgcggagg cggcggcgga ttggcacggc cttgatttcc
     5701 gaacgggtcg gtctggccga tcaaccgggc cagctcagcc aatgggtcgc tctcgccccg
     5761 tccaggctga tcgccgccgc gattgtaatc gtcggcgggg aaaggtcggt tctgatatcg
     5821 gtgagccatc gtgatggtgc gtccctctgg gaaggctgcc ctgtcaaatt acaggcgaac
     5881 tggcccccgg atagccccaa ggttcaacgc aacaggcggc cagttcccca cattaccgca
     5941 tttgctcggg agcatggacg cccaaaatgc ctagtcccga ggacaaaacc gagactacgc
     6001 cctggaccat cgccagccgc gccttcgtga tccctgcatc attattgata atgaagcgta
     6061 aattgggcag gtcgtggcct ttgttccaga gcgcatgaaa ctcgctggcc agatcataca
     6121 ggtaaaaggc gattcggtgc ggctcatgtg cggccgcggc gccctcgatc gtccgaggat
     6181 agagcgccaa ctgcttgaga aggccgatct cggccggatc ggtcaatcgc tcgaccggcg
     6241 cggtaccaag gtatgccagg cgcgcctcgg ttcccaccgg gagatcgggc agcacctcct
     6301 cccgcgcgtt gcggaagatc gaatggccgc gtgcgtggcc atactgaacg taaaacaccg
     6361 gattgtcgcg tgactgctcg atcaccttgg cgaggtcgaa gtccagcacg gcgtcgttct
     6421 ttcgataaag catcatgaag cggacggcat ccttgccgac ttcgtcgacc acctcccgca
     6481 aggtgacaaa gtctcccgcg cgctttgaca tcttcaccgg ctcgccggcg cgcaggagtc
     6541 tgacgagctg gacgattttg acgtcgagcg cggcctcctc gccggtgacc gccttcactg
     6601 cggactgcac gcgcttgata tagccgccat ggtcggcgcc ccaaacgtcg acgagatcac
     6661 gaaagccgcg gtcgaacttg ttcttgtgat aggcgatgtc ggaggcgaag taggtgtagg
     6721 tgccgtccga cttgatcagc ggacgatcga cgtcgtcgcc gaacgcggtg gcgcggaaca
     6781 gcagttgctc gcggtcctcc caatcctcga caggcgttcc cttcggcggc ggcaggcggc
     6841 cttcatagac gtcgcctcgt gagcgcaaat agtcgatggt ctcggcaacc ttattgttac
     6901 cgccttcgat cagcgagcgc tcggagaaga atacgtcgtg cctgatgttg agggctgcaa
     6961 gatcgtcctt gatctcgtcc atcatcatgg cgatggcctt ggctcgcacg atcggcagcc
     7021 actctgcctc gctcatcgca tggagcttgt cgccatattc tgacgcaagc gcctgcccga
     7081 ccggcttcag ataatcaccg ggataaagcc cgtccgggat cgcgccgatc ccctcgccaa
     7141 gcgcttcgcg gtaacgcaga taggccgagc gcgcgaggac atcgacctgg gcgccggcgt
     7201 cgttgatgta atactcgcgc gtcacgtcgc ggccggcgaa ttgcaacaag cttgccagcg
     7261 catcgccaaa cactgcaccc cggcaatggc caacatgcat cggaccggtc gggtttgccg
     7321 agacatattc gacattgacc cttgggctgg catccggcgc gatccggcca tagtcatcgc
     7381 cttctcccaa caccgtgagc agcgcctcgg cccaagcctt cggcttcagc gtgagattga
     7441 tgaagccggg tcctgcgaca tcgacctttg cgatcagttc gtccgcacct aggcgcgcgg
     7501 cgatcgcttc cgccagatcc cgcggtttgg attttgcctc cttggcgaga accatcgcgg
     7561 cgttagttgc catgtcgcca tggctggcgt cgcgcggcgg ctcgaccacg atgcgcgaaa
     7621 aatcgatacc gtccggccag cggccttcag tcgcaaggct tgcgcagatc gcgtgcacgc
     7681 gcgcaagcac gtctgcgaaa agatgaagcg atgatgattt ttcggccatg cgccgccgcc
     7741 taacgcaatt caggggtcaa gtcaaaaagc ctctgatgct ccatcaaggc aaaacggtcg
     7801 gtcatgcccg cgatgaaatt accgatccgc cgcgcgcgcg cagattcgcc ttctctctcg
     7861 gccccctcaa gccattcggg cggcagatcg ccggggatcc tttgatagcg tgcgaacagg
     7921 tcgagcacga tctgctcggc ctccgccatc acgcgcatta cgcgagggtg gcgatacatg
     7981 cgctgcctca ggaacgcctt gatgccggct tccgcgtccg cgatgtcggg ggaaaatgca
     8041 ataagctgat ggccgcagtg gcggacgtcg tcgaccgatt tcggctgcac gatcgctatc
     8101 cggcggcctg attccgaagt caccgccccg atcaggcgcg agatcagttc gcgcaccagt
     8161 tccgcaccac gccggtcttg atcgagcccg ggatagcggc tgtcgatatc ggcgaccatc
     8221 tcggcggtca gtggcatgac cttgaggtcg tcgacggcaa acaggccggc gcgcaaaccg
     8281 tcatcgatgt catgggcatc gtaggcgatg tcgtcggcga tcgcggccac ctgggcctcg
     8341 agcgatgcaa agcctgacag ctcgagattg tagatcgcgt cgaattgggc aatgcccacg
     8401 ggaatgccgg cctcgcgata atgaccgagt gcagcgccac tcgcgtcgat caacgggccg
     8461 ttgtgcttga cgatcccttc cagcgactcc caggtcagat tgagcccgtc gaaattggga
     8521 tagcgatgtt ccagcatggt gaccacgcgg agcgtctgcg cgttgtgatc aaagccgcca
     8581 tgcgccttca gacaggcgtc cagtgcgcgc tcgccggcgt gtccgaacgg gggatggccg
     8641 agatcgtgcg ccagcgccaa tgcttccgtg atgtcctcgt cgagcccaag ttgccgcgcc
     8701 agcgcgcggg cgatctgcgc gacttccagc gaatgggtca ggcgagtgcg gtagtgatcg
     8761 ccttcgtgga atacgaacac ctgggtcttg tgcttcagcc ggcggaaggc ggtggaatga
     8821 atcacgcggt cgcaatccct gcggaaggga ctccgcgtct tgctgggcgg ctcgacgaat
     8881 tgccggcccc ggctgcggtc ggggtcgcag gcatagggtg cgcggggggc agccattccg
     8941 accgacacgg tgtattcatc ctcttcgagg tttgattctt gcggcgccag cacttaacta
     9001 tgtcgtgcgt gggataccaa atgaataggg tatgaagcac cgaccggagt tgcatctgcg
     9061 atgaccactg ccgtcaccat cagcgagcgg gccgcccgtc gcatcggcga aattctgacc
     9121 agggagggcg aaggcgccat gctgcgcatc tcggtcgaag gcggcggctg ctccggtttc
     9181 caatacaagt tcgatgtcga gcatgcgaag gccgaggatg acctggtgat cgagcgcgat
     9241 cacgcggtcg tgctgatcga tccggcttcc gttccgttcc tggcaggatc agaggtcgat
     9301 ttcgtcgacg atttgatcgg cgcctccttc cgcgtgatca atccgaacgc caccgcgtcc
     9361 tgcggctgcg gtaccagctt ttcgatctga cgcttgacgc gcatgcgcat cgccacctgg
     9421 aacgtcaatt cgatccggca gcgcatcgag ccggtcatga gctggctgaa agagacgtcg
     9481 cccgacatcg cctgcctgca ggaaatcaaa tgcacagacg atgcttttcc gcggctcgaa
     9541 ttcgagtcgc tcggctataa cgtcgtcacc cacgggcaaa aaacctttaa cggcgtagca
     9601 ctcctctcaa agctgccgtt cgatgagacc aagtcgggcc ttgccggcga cgatgaggac
     9661 gcacacgccc gctttctgga gggcgtggtc actctgaaaa gcggtgtttt gcgagtcgcc
     9721 tgcctctatc tgcccaacgg caacccgccc gataccgaca aatatcctta caaactcaat
     9781 tggatgtcgc gcctctttga gtattcgaag caacggctta aagccgaaga gccgctcatt
     9841 ctggccggtg attttaacgt catccctact gccgaagacg tttataaccc gccggcctgg
     9901 gtcaatgacg ccttgttccg caccaccacc cgcgagagct ttcaggcact gctcggactc
     9961 ggcctcaccg atgcactgcg tgccgtcaac gacgcggcag ggcagtacac attctgggac
    10021 tatcaggccg gcgcctggca gaaaaactgg ggcctgcgca tcgatcatct tctgctgtcc
    10081 ccgcaggcga gcgaccggct gatcgatgtc gggatcgaca gttatgtccg cgcctgggag
    10141 aagccgtcgg accacgtacc ggtgtgggcc gacctggacc tcgaagcgcg ataggtttgg
    10201 agaggttcga tcagtcccgg cggccctgca cccagtgctc gagcatctgc agggccatgg
    10261 cgcgatcgtt atccgaagcg ctggcgatcg cgcggttgta gctttccttg atccagcttt
    10321 cgtcgggacc ggcgttctcg catgcaagcg tcagccacat caggccacgc gcgcgttgcg
    10381 gcgggatgcg gtcgccattg aacaacatct gcccgagcat cgcctgcgcc tggtgctggc
    10441 ccttttgcgc cgcaaggccg agccagcgcg caccataggt aaactcgctc ggcgtcgcat
    10501 tggcggtctt caggtagagc cgggcgagat catattgcgc gtcggcattt ccgaaatagg
    10561 aggccgcgta atagaacatc tc
//
LOCUS       DATLJT010000966         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1903126,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000966 DATLJT010000000
VERSION     DATLJT010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903126"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1300
                     /locus_tag="VM910_22235"
     CDS             131..1300
                     /locus_tag="VM910_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HVI65276.1"
                     /translation="MFIKVDPISLVGQISPRSLQGAIALAYCADASDKDGGIRFAIPP
                     YELCAPGITAVVYFAVRRMRFVQHGTAGEKQMQVANLVLPVFAIILTGWLAGWLGYIS
                     RSLSDGLVHFAYNVAMPALLVVTIAQEPARSLLEWRFLFAFGGGSIFCFLLVFLAVYT
                     GLGRGVASSTMHGMAAAMTNTGFVALPILHSIYGQPAVLPAAVATVFVAAVMFPATVI
                     LLESEGRGDDGRARPVALMKQIVLNPMVLSTLIGLAWAITGLPMPLPLKAYLNIFAAA
                     LTPCALFAIGLGLSVEGITSNLTMSLVLAAVKLLIMPLIVYGLCAASNLDPLYTIAAV
                     VCAAVPTAKTAYILAGEYKVEEELVAATVSITTLLSIATLLGWLYALSRLAGQAS"
     gene            complement(1337..>2618)
                     /locus_tag="VM910_22240"
     CDS             complement(1337..>2618)
                     /locus_tag="VM910_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI65277.1"
                     /translation="LKRLRDLGNTVIVVEHDEDAIRLADHVLDIGPGAGMHGGNIVAQ
                     GTPADIMHSPASLTGKYLTGELSVAIPERRPPNHRRTIKVVNARGNNLKNVTAEIPLG
                     LFTCVTGVSGGGKSTLLIDTLYRAIARKLNGASEGAAPHDRIEGLEHIDKIIDIDQSP
                     IGRTPRSNPATYTGAFTPIREWFAGLPEAKARGYEPGRFSFNVKGGRCEACQGDGVIK
                     IEMHFLPDVYVTCDVCKGKRYNRETLEVLFKGKSIADVLDMTVEEAAEFFKAVPRVRE
                     TFNTLHRVGLDYIHVGQQATTLSGGEAQRVKLAKELSKRATGRTLYILDEPTTGLHFH
                     DVKKLLEVLHELVAQGNTVVVIEHNLEVIKTADWVIDLGPEGGDGGGEIVAWGPPEDI
                     VKAPRSYTGKFLGPVLAKAGKRKRGGGGASEAAE"
BASE COUNT          423 a          821 c          824 g          550 t
ORIGIN      
        1 catttctgaa aaacggaagc ataattttgc gattcatgtc tgaacggccg gctcagcgct
       61 gaaccgcttc acgaatttcg atttttcgcg caggtgattt cgcgcgcaaa ggggctgcgt
      121 tgtcgatgtg atgttcataa aagtcgatcc gatttcactt gtcgggcaaa tcagtccacg
      181 aagtttgcag ggcgcaatag cgctggcgta ttgcgccgac gcctcggaca aggacggcgg
      241 aatacgcttc gctattccgc cttacgagct ctgcgctccc ggtatcactg ccgtggtata
      301 cttcgctgtg cgccggatgc gcttcgtcca gcacggcacc gccggggaga agcagatgca
      361 agtggccaat ctggtcctgc ccgtatttgc gatcatcctt accggctggc tcgccggatg
      421 gctcggctac atatcgcgat cgctctccga cgggctcgtg catttcgcct acaatgtcgc
      481 gatgccggcg ctgctggtcg tgacgatcgc gcaggagccg gcgcgcagtc ttctggaatg
      541 gcgctttctg ttcgcattcg ggggcggctc catcttctgc ttcctgctgg tttttctggc
      601 ggtttacacc ggactgggac gcggcgttgc cagcagcacc atgcacggaa tggcggcggc
      661 catgaccaat accgggttcg tggcgctgcc gatccttcac tcgatctacg gccagcctgc
      721 cgtgctgccc gcggccgtcg caaccgtgtt cgttgcggcg gtgatgtttc ccgcgacggt
      781 cattctgctg gagagcgagg ggcgtggcga cgacggaagg gcccgcccgg tcgccctgat
      841 gaagcagatc gtgctcaatc cgatggtgct gtcgaccctg atcggtctgg cgtgggcaat
      901 cacgggcctg ccgatgccgt tgccgctcaa agcgtatctg aacatcttcg ccgccgcgct
      961 cacgccctgc gcgctcttcg ccatcgggct tggcctgtcg gtcgagggga tcacctcgaa
     1021 cctcacaatg tcccttgtgc tcgcggccgt gaagctcctg atcatgccgt tgatcgttta
     1081 cgggctgtgt gccgcctcaa acctcgatcc gctctatacg atcgcagccg tcgtctgtgc
     1141 cgccgtgccg accgccaaga ccgcgtatat tctggcaggc gagtacaagg tggaggaaga
     1201 gctggtcgca gccacggtct cgatcacaac gctgctgtcg atcgcgacgc tgctgggatg
     1261 gctgtatgcg ctttcacgac tggcggggca ggcgagttag agccaccgac tttcttgggc
     1321 atcgttcaat taaacctcac tccgccgcct cgctggcgcc gccaccgccg cgcttgcgct
     1381 tgcccgcctt cgccagcaca ggacccagga acttgcccgt gtagctgcgc ggcgctttca
     1441 caatgtcttc gggcggaccc caggcaacga tctcgccgcc gccgtcgccg ccttcggggc
     1501 cgagatcgat cacccagtcc gcggtcttga tcacctcgag attgtgctcg atcaccacga
     1561 ctgtgttgcc ctgcgcgacc agctcgtgca gcacttccag aagctttttc acgtcgtgga
     1621 aatgcaaccc cgtggtcggc tcgtccagga tatagagggt gcggcctgtg gcgcgcttcg
     1681 acagctcctt cgcaagcttc acgcgctgcg cttcgccgcc ggaaagcgtc gtcgcctgct
     1741 ggccgacatg gatatagtcg aggccgacgc gatgcagcgt gttgaaggtt tcgcgcacgc
     1801 gcgggacagc cttgaagaac tcggcagcct cttccacggt catgtcgagc acgtcggcaa
     1861 tcgacttgcc cttgaacagc acctccagcg tctcgcggtt gtagcgcttg cccttgcaga
     1921 cgtcgcaggt gacgtagacg tccggcaaga agtgcatctc gatcttgatg acgccgtccc
     1981 cctggcaggc ctcgcagcgg ccacccttga cgttgaagga gaagcgtcca ggctcgtagc
     2041 cgcgcgcctt cgcctcggga agacctgcga accactcgcg gatcggggtg aacgcgccgg
     2101 tgtaggtcgc ggggttggag cgcggggtgc ggccgatcgg cgactggtcg atgtcgatga
     2161 tcttgtcgat atgctccagc ccctcgatgc ggtcgtgagg ggcagcaccc tcgctcgcgc
     2221 cgttgagttt tcgcgcgatg gctcgataaa gggtatcgat cagaagcgtc gacttgccgc
     2281 cgccggacac accggtgacg caggtgaaca ggccgagcgg gatctccgcg gtgacgttct
     2341 tcagattatt gccgcgcgca ttcaccactt tgatggtgcg gcgatggttc ggcggccgcc
     2401 gttcggggat ggcgaccgac aactcgccgg tcagatattt gccggtgagg gaggccggac
     2461 tgtgcatgat gtcggcgggc gtgccctggg ccacgatatt gccgccatgc atgccggcgc
     2521 cgggaccgat gtcgagcaca tggtcggcaa ggcgaatggc atcttcgtca tgctcgacca
     2581 cgatcacggt attgccgagg tcgcgcagcc gtttcagc
//
LOCUS       DATLJT010000967         2738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2822252,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000967 DATLJT010000000
VERSION     DATLJT010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822252"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VM910_22245"
     CDS             complement(<1..330)
                     /locus_tag="VM910_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVI65278.1"
                     /translation="MSEPSTGATGARDKPWIFRTYAGHSTAHDSNALYRKNLAKGQTG
                     LSVAFDLPTQTGYDSDHALAKGEVGKVGVPISHLGDMRTLFDHIPLGAMNTSMTINAT
                     AAWLLALY"
     gene            complement(584..811)
                     /locus_tag="VM910_22250"
     CDS             complement(584..811)
                     /locus_tag="VM910_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVI65279.1"
                     /translation="MLIVGAIAGWLAGQIVRGFGFGLLWNIVIGIVGAFIGVWLLMQL
                     GFVPFSGFVGSIVNAVIGAVVLLVIVGFIKR"
     gene            1071..2354
                     /gene="ccrA"
                     /locus_tag="VM910_22255"
     CDS             1071..2354
                     /gene="ccrA"
                     /locus_tag="VM910_22255"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HVI65280.1"
                     /translation="MPAVAITKPPQAVRKDLYGIGEVPPLGHVPARMHAWAIRKDRHG
                     PPETSMQLEVLPTWTIGDDEVLVYVMAGGVNYNGVWAGLGIPISPLDVHKHPFHIAGS
                     DAAGIVWACGSKVRRWKVGDEVVVHCNQDDGDDEECNGGDPLLSASQRIWGYETPDGS
                     FAQFCRVQSRQLMPKPAHLTWEEAACYTLTLATAYRLLYGHPPHTLKPGDHVLVWGAS
                     GGLGVFGVQLAAASGANAIGIISDESKRDYVMQLGAKGAINRKQFSCWGQMPKVGTPD
                     YDTWVKEARKFGKAVWDITGKRDVDIVFEHPGEATFPVSCLIVKRGGMVVFCAGTSGY
                     NLTFDARYVWMRQKRVQGSHFAHLKQASAANQFVLDRRIDPCMSEVFSWTRIPHAHEL
                     MRTNRHPPGNMAVLVNTPRAGLRTLDDVIEAAATP"
     gene            2466..>2738
                     /locus_tag="VM910_22260"
     CDS             2466..>2738
                     /locus_tag="VM910_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65281.1"
                     /translation="MCDYSLHNVKSRAARVGDKLTTRNFGTGTRGFAAQEDTTMAVCV
                     LPGTELAFSREVACTASGFLGLGARRMAYQTAIFRQVNKDQPRVHHD"
BASE COUNT          522 a          865 c          877 g          474 t
ORIGIN      
        1 atagagcgcg agcagccagg cggccgtcgc gttgatggtc atcgacgtgt tcatcgctcc
       61 gagcgggatg tggtcgaaca gcgtgcgcat gtcgccgaga tgcgagatcg gcacgccgac
      121 cttgccgact tcgcctttgg ccagcgcatg atcgctgtcg tagccggttt gcgtcggcag
      181 atcgaaagcg acggaaagac cggtctgacc cttggcgaga ttcttgcgat agagcgcgtt
      241 cgagtcgtgc gcggtcgaat ggccggcata ggttcggaag atccagggct tgtcgcgcgc
      301 cccggtcgcg ccggtcgatg gctcgctcat gaaagccgcc tcgctgagcc tcgcgattgt
      361 gcggcgcagc atactccgag cgcagtcgaa agggtagccg cttgcaaccg cctatcccgc
      421 cgtccccgca ggcaaggcgg aggtctcagc gacatagatg gtcggcgctc gctcgcaaat
      481 gcctaacgcg cggacgtggc tcagcgccta tccgcgtgct taggctcagg gctttcgctt
      541 gcctggtcgt ggcaacccgg tcggcgggag cgctgccgtc cgactagcgt ttgatgaacc
      601 cgacgatgac caaaagaacg acggcaccga tcacggcgtt gacgatggag ccgacgaaac
      661 cggagaacgg cacgaacccc aattgcatga gcagccagac gccgatgaag gcgcccacga
      721 tgccgatgac gatgttccag aggagtccga agccaaaacc gcgcacgatt tgcccggcca
      781 accagccggc aatcgcgccg acgatcagca tgatgataat gctgtctatt ccaaccacga
      841 tgtcctcccc gacacggccg ggcacgagcg tcccgccgcc actttgcgat gcaacttagg
      901 atgcaactta gaaagcgtag cccaagtcga tcaaactgtc agcaaccgcc ggcgcaccgg
      961 tggacaaggt ctgcgatcga gggtgcgcgt gccgcaatcc ggcgtcgata ttgcgatgca
     1021 gcatcaaatg cggcagactg gcgcttgcgt tgcaagttgc ggaggcagcc atgcccgccg
     1081 tcgcgataac gaagccgccg caggcggtgc gcaaggacct ctacgggatc ggcgaagttc
     1141 ctccgctcgg ccacgtgccg gcgcgcatgc atgcctgggc gatccgcaag gatcgccatg
     1201 gcccgccgga aacatccatg caactcgaag tgttgccgac atggacgatc ggcgacgacg
     1261 aggtgctggt ctacgtgatg gccggcggcg tcaactacaa cggcgtatgg gccggcctcg
     1321 gcatcccgat ctcgccgctc gacgtccaca aacatccgtt ccatatcgcc ggctccgatg
     1381 ctgcgggaat cgtttgggct tgtggttcga aggtgcgccg ctggaaagtg ggagacgaag
     1441 tcgtcgtcca ctgcaatcaa gacgacggcg acgacgaaga atgcaacggc ggcgacccgc
     1501 tgctctctgc ctcgcagcgc atctggggct acgagacacc ggacggatca ttcgcgcagt
     1561 tctgccgcgt gcagtcccgt cagctcatgc cgaagccggc gcacctgact tgggaggagg
     1621 cggcctgcta cacgctgacc ctcgctaccg cctatcgctt gctctatggc catccgccgc
     1681 acaccctgaa gcccggcgac catgtgctgg tgtggggcgc ctccggcggc ctcggcgttt
     1741 tcggcgtgca gctcgcggcc gcttccggcg ccaacgccat cggcatcatc tcggatgaaa
     1801 gcaagcgcga ctacgtgatg cagctgggcg ccaagggcgc gatcaaccgc aagcagtttt
     1861 cctgctgggg ccagatgccg aaggtcggca cgccggacta cgatacctgg gtgaaggagg
     1921 cgcgcaagtt cggcaaggcg gtctgggaca tcaccggcaa gcgggacgtc gatatcgtct
     1981 tcgagcaccc cggcgaggcg acgttcccgg tctcctgttt gatcgtgaag cgcggtggca
     2041 tggtggtgtt ctgtgccggc acctcgggct acaacctcac cttcgacgcg cgctatgtgt
     2101 ggatgcggca gaagcgcgtg cagggctcgc acttcgccca cctcaagcag gcctccgcgg
     2161 ccaaccagtt cgtgctcgac cgccgcatcg atccctgcat gagcgaggta ttttcctgga
     2221 caagaatccc gcacgcgcac gaattgatgc gaaccaaccg gcacccgccg ggcaacatgg
     2281 cggtgctggt caacacgccg cgcgcggggc tgcgcacgtt ggacgatgtg atcgaggcag
     2341 ctgcgactcc ataggcggcg tagttcgtaa tcttctttcc accgcttgcc ataagaatgg
     2401 cacctttctt gcttcgaatc acacaggtgg acggtacggg gggcttggaa gagaggagcc
     2461 tcaccatgtg cgactacagc ctacacaacg tgaagtcgcg tgctgcgaga gtcggcgaca
     2521 agctcacgac gcgcaacttt ggtaccggca cacgtggctt tgccgcccag gaagacacca
     2581 ctatggcggt ttgcgttctt ccgggaacgg agctcgcgtt ttcccgggag gtcgcatgca
     2641 cggcgtccgg attcctcggc ttgggcgcgc gcagaatggc ctaccagacg gcgatctttc
     2701 ggcaggtcaa caaggaccaa ccgcgggtgc accatgac
//
LOCUS       DATLJT010000968         3335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_64881,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000968 DATLJT010000000
VERSION     DATLJT010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64881"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VM910_22265"
     CDS             complement(<1..195)
                     /locus_tag="VM910_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HVI65282.1"
                     /translation="MADFDAMMTRRCWLIAGAASLLQTGALFAATEALITVHKDPTCG
                     CCSGWVQHLHDAGFVTKVLDT"
     gene            324..662
                     /locus_tag="VM910_22270"
     CDS             324..662
                     /locus_tag="VM910_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65283.1"
                     /translation="MSNGRTILALLIGLSVAMLPAAGGAGIKSPAPAGMSAMEDMSDC
                     CPQNADPCAKAMDHCGTMATCALKCFSFVATASSIVVFPSTFASMVIPPAADPFASQT
                     GSPPFRPPRI"
     gene            795..1499
                     /locus_tag="VM910_22275"
     CDS             795..1499
                     /locus_tag="VM910_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI65284.1"
                     /translation="MNRIQPLAIALLLLTAGRAMAEPGNAARGERMYRACVACHSLEP
                     NRNMTGPSLAEIWNRTAGSLASFPRYSPALKSAGIIWTDDTLDEWIKDPQHFIPGNTM
                     TFPGMKDARQRADLLAFLKEATQPGHAPKAAQDGGQMGGMMMGGGGAPNLKKLDPQDR
                     VQSINHCGDTYKVATADGKVRDFWERNLRFKTDVSDDGPQKGAPALVGAGMMGDRADV
                     IFAGPEEISGFIANAC"
     gene            1605..2195
                     /locus_tag="VM910_22280"
     CDS             1605..2195
                     /locus_tag="VM910_22280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI65285.1"
                     /translation="MLALDQDSLERRSLARNEVLFRQGDKVTAIYFIEEGRLRLERRT
                     FDGRLLFLGTTPAGEFFVEAALFSDTFHCDAVAAEPSRVRVYPKAMVLNCLRTDPASA
                     MSFLADVAHQIIELRQRLELMKVRSAKARLMLYFESNAEPGGRTVKLRGQLQDIASDL
                     GLTREALYRTLASLERAGAIERAGARILLKKSVGPR"
     gene            complement(2380..2928)
                     /locus_tag="VM910_22285"
     CDS             complement(2380..2928)
                     /locus_tag="VM910_22285"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI65286.1"
                     /translation="MIRMALFAVVAIVAATATSSAQTTSPYAGQEQRAIKAFSDADIG
                     DLLEARGMGLAKAAELNSYPGPLHVLQLANELGLSDAQRKATESLYATMRERALSIGR
                     QIIEAERTLDRAFANGAIELATLRSQVRAIATLQGELRAVHLETHIAQRALLTPEQIA
                     RYDVLRGYRGSQAPHDTRRHGG"
     gene            complement(3042..>3335)
                     /locus_tag="VM910_22290"
     CDS             complement(3042..>3335)
                     /locus_tag="VM910_22290"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI65287.1"
                     /translation="TFIGPLSTTIVVGWDGVRKAWEMRFGQFDRVTISLAESHVRTNG
                     KVAWAVGIEKVELLRKDGKTSSFDAFVTNVFEQRDGRWLLVSHQATPTFRAAE"
BASE COUNT          635 a         1055 c         1038 g          607 t
ORIGIN      
        1 ggtgtccaac accttcgtga cgaagcccgc atcgtgaagg tgctggaccc aacccgaaca
       61 gcagccgcag gttggatcct tgtgaaccgt gatgagcgct tccgttgctg cgaagagcgc
      121 cccagtttgc agcagggagg cagcgcccgc gataagccag catcgccgcg tcatcatcgc
      181 atcgaaatct gccatggcgg gcctcggtat ggcatcatca agttggccaa gcggttagtc
      241 cactagaatt tagcggttac gtcgcgcatt cgtgccttga ccttgggacc gaggaacctg
      301 ctattttcgt caccgcccga gccatgagca acggtcgaac cattctagcg cttctcatcg
      361 gcctctcggt cgccatgctg ccggcagccg gcggtgctgg cattaaatcg cccgcgccgg
      421 caggcatgtc cgcgatggag gacatgtccg actgctgccc ccaaaacgcc gatccgtgcg
      481 cgaaggccat ggatcattgc ggaacgatgg caacctgcgc gctcaagtgc ttcagcttcg
      541 tggccactgc gtcgtcgatc gtcgtattcc cctcgacctt tgcgagcatg gtgatcccgc
      601 ctgccgccga cccgttcgcg tcgcagacag gcagtccgcc cttccgacca cctcggatct
      661 gacatctccc tgaagatgtg acgtcgcgcg tgagcgcaaa gctcgcgcga aatgcgttcg
      721 gccgcggccg gcacgcatgt tcgatcgtct tttgaggcag cgagtgacaa ccgaggaatc
      781 gatggaggtc gacgatgaac cgaatccagc cccttgcgat cgcactactc ttgctgaccg
      841 cgggccgagc aatggccgag ccgggcaatg ccgcgcgcgg cgagcgcatg taccgcgcct
      901 gcgtcgcctg ccactcgctc gagcccaacc gcaacatgac cggcccgagt ctcgcggaaa
      961 tctggaatcg gacggcggga tcgttggcga gcttcccgcg ctactccccc gcactcaaat
     1021 cggccggcat catctggacc gacgacacgc tcgacgaatg gatcaaggac ccgcagcact
     1081 tcattccggg aaacaccatg acgtttcccg gtatgaaaga cgcgcgccag cgcgccgatc
     1141 tgctcgcctt tctcaaagag gcaacgcagc ccggccatgc gcccaaggcc gcgcaagacg
     1201 gcggccagat gggcggcatg atgatgggcg gcggcggggc gccgaatctg aaaaagctcg
     1261 atcctcagga ccgcgtgcaa tcgatcaatc attgcggcga cacctataag gtcgccaccg
     1321 cggacggaaa ggtgcgcgat ttctgggaac gcaacttgcg cttcaagacc gacgtcagcg
     1381 acgatggccc gcaaaagggc gcgccggcgc tggtgggagc cggtatgatg ggagaccgcg
     1441 ccgacgtgat tttcgccgga cccgaggaga tcagcggctt catcgcgaac gcgtgctgaa
     1501 agcgagttcg gcccaatcgt cagcgcgcaa aatccagggc cgctcgtggc gccgtgttcg
     1561 agggtcaagc cgcacccttc ggcggccaat gtccggatat gggtatgctg gcgctcgacc
     1621 aagactcgct tgaaagacga tcactcgctc gaaacgaggt gctgtttcgg caaggcgata
     1681 aagtgacggc catttatttc atcgaagaag ggcggctgcg gctggaacgg cgcaccttcg
     1741 acggccgatt gctgttcctc ggcaccacac ccgcgggcga gttctttgtc gaagcggcgc
     1801 tcttttccga caccttccat tgcgacgccg tggcggcgga accctcgcgg gtgcgcgtgt
     1861 atcccaaggc catggtgctg aattgtcttc gcaccgaccc cgcgagcgcc atgtcgttcc
     1921 tggcagacgt ggcgcatcaa atcattgagc tgcggcagcg cctcgaactc atgaaagttc
     1981 gctccgccaa agcgcggctg atgctctact tcgagtccaa cgccgaacct ggcggacgca
     2041 cggtcaagct gcggggccag ctgcaggata tcgcgagcga tctgggcctg acccgcgagg
     2101 cgctgtatcg gacgcttgcc agcctcgagc gcgctggcgc catcgaacgt gcgggcgccc
     2161 gcatcctgct gaagaaatcg gtcggcccgc gctaggagcg caggtacttg atgaacgccg
     2221 cgattcccag aagcaggaag atgatcacga ggagccaaat gaggcccatt ccccacatca
     2281 tcgctatggg catgtcggtc atctgcatca tgttcgcacg ctcgctaatt cgccttcgca
     2341 atcgtgcaca aggaagctaa tgagcgcaat tgcgttgctc tagccgccat gacgacgggt
     2401 gtcgtgcggc gcttggctgc ctcgatatcc gcgaagcacg tcgtaccggg cgatttgttc
     2461 cggcgtcagc agcgcgcgct gagctatgtg ggtctccagg tggacggccc gaagctcgcc
     2521 ctgcagggtc gcgatcgcac ggacttgaga gcgaagcgtc gccagctcaa ttgcgccatt
     2581 ggcaaaggcg cgatcgagcg tccgctcggc ctcgatgatc tggcggccga tggataaggc
     2641 ccgttcgcgc atagtcgcat agagcgactc ggtcgccttg cgctgcgcgt cggacagtcc
     2701 aagctcgttg gccagctgca gcacgtggag cggtcccggg taggagttga gctcggcggc
     2761 tttggccaag cccatgccgc gcgcctccag caggtctccg atgtcggcgt ccgaaaacgc
     2821 cttgatggcg cgctgttcct ggccggcata gggcgacgtg gtttgagcag acgaagtggc
     2881 cgtcgccgcc acgatggcga ccaccgcaaa gagtgccatg cgaatcatga tcgcctcccg
     2941 tggttagaga cgcgatcatg cgctcgggac cgatacctgg atatgattac gatcatatcc
     3001 cgagcttggg atacttcgct cgagcccaga aggttcacgc actactcggc tgctctgaag
     3061 gtcggcgtcg cttggtgaga caccaagagc cagcgaccgt cgcgctgctc gaatacgttc
     3121 gttacgaagg cgtcgaagct cgaggttttg ccgtccttgc gcaggagctc caccttctcg
     3181 ataccgacgg cccacgcgac cttcccgttg gtacgaacgt gggattcggc caaggagatg
     3241 gtgacgcggt cgaactggcc gaagcgcatt tcccaagcct tcctgacgcc gtcccagccg
     3301 accacgattg tcgtgctgag gggaccgatg aatgt
//
LOCUS       DATLJT010000969         1766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1903247,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000969 DATLJT010000000
VERSION     DATLJT010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903247"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..740)
                     /locus_tag="VM910_22295"
                     /pseudo
     CDS             complement(148..740)
                     /locus_tag="VM910_22295"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(894..>1766)
                     /locus_tag="VM910_22300"
     CDS             complement(894..>1766)
                     /locus_tag="VM910_22300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI65288.1"
                     /translation="QVSATLTLLTWLAARTTTLRLGTAVMVLPWHNPVLLAEQAATLD
                     LLSEGRLDFGVGKGYRYNEFTGFAIPLDEADARFEEALAVIIKAWTSGQRFSHHGRFW
                     TFNDILVEPPTSQKPHPPFWMAAGTPPSVRRVAERGYNLLLDQFAAFDVIGERITIFK
                     AERAACGCAYNPMGIAVARDMHVANNQAAKDAALERNRQFHERILAVARWPDRTVGSH
                     ILAYSHAPEEAEANSLVGTPDEIMGKLEALRRAGIEYVILSCGGSRESLRRFAHEIMP
                     AFSDEPAKVRANLV"
BASE COUNT          331 a          548 c          566 g          321 t
ORIGIN      
        1 aggcgcttcc acagatgaac gacggtcaat taacgcatcc cgaacctgac ccggcgcctt
       61 tcaaggtcca tctactcaca agagctgggc gcgccatgcg cacgcgcagc gagccggtgc
      121 gagcgttttc cactaaggac gaagccgtca gatttgcggg tcgccagctt ggcgggaact
      181 acgcggtgta ccacaaggac aggaaaattt ggcccatctc gcaggacgcg cctcgctgag
      241 gttgcgcgtt gtgcgaagcc ccggccctcg cccgcgatga tgctcgacgc tgagctcaac
      301 gatttcggcg cgcaactcgc catgctcggc ggtcacgcgg ccgactgtga tgggtaggcc
      361 gaagcggcgc ggcccggcac tgcccggatt gaaaaagagt gtgccgttct ttgtctcgat
      421 cttgggcagg tgcaaatgac catagatcac cgcgtcgacg tttgcggcgg cctgggggtc
      481 aagctcggcg agaatgtgta gcacgtaaat gcggaagcca tcgatctcga tccgctgcgt
      541 cacagatacg ttcgcgctcc aagcgccgtg atcgacgttt ccgcgcacgg cgtggacggg
      601 cgccagcgca gcctcgcaag tacgtcaggg ctgccgacat ccccggcatg gatgatggga
      661 tcgcagccgg caagcgccgc tagcgcctgc ggccgcagca ggccatgggt atcggaaatc
      721 gcgccaatgg ttcgcatcat gcccgatgcc actccagcaa actttatccg gcgcgacagg
      781 gcctgtcgct caattgcatc cgacggcgca accacaatag cgcggtcaca ttgcccgtcc
      841 ttgctctctt ccagcgagta agttgacttg aaccacgcac ctgccggaca actttatacg
      901 aggttggctc tgactttcgc tggctcatcg gaaaaggccg gcatgatctc gtgcgcgaaa
      961 cgacggaggc tctcccgcga gccgccgcag ctcaaaatca cgtattcgat gccggctcga
     1021 cgcaaggcct cgagcttgcc cataatctcg tctggcgtgc cgaccaggct gttcgcctct
     1081 gcttcttcgg gcgcatgact ataggctagg atatgcgagc cgacggtgcg gtccggccaa
     1141 cgcgccaccg ccaaaatgcg ttcatggaac tggcgattgc gttcgagggc ggcgtccttg
     1201 gcggcctggt tgttggcgac atgcatgtcg cgcgcaacgg caatccccat tggattgtag
     1261 gcacacccac acgccgcgcg ctcggccttg aatatggtaa tgcgctcgcc gatgacgtcg
     1321 aacgctgcga actggtcgag gagcagattg tagccgcgct cggccacgcg gcgaacggac
     1381 gggggggtcc cggcggccat ccagaacggc gggtgcggtt tttggctggt cggcggctcg
     1441 accaggatgt cgttgaaggt ccagaagcgg ccgtggtgcg agaagcgttg tcctgaagtc
     1501 catgctttga tgatgaccgc aagcgcctcc tcgaagcgcg catcggcctc gtcgagtggg
     1561 atcgcgaagc ccgtgaactc attgtagcga taacctttgc cgactccgaa atcgagccgg
     1621 ccctccgaca ggaggtccaa ggtcgcggcc tgctcggcca ggagcactgg attgtgccac
     1681 ggcagcacca ttacggcagt gccgagccgc agcgtcgtgg tgcgcgccgc gagccacgtc
     1741 agcagggtga gggtggccga gacttg
//
LOCUS       DATLJT010000970         7628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_295406,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000970 DATLJT010000000
VERSION     DATLJT010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295406"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VM910_22305"
     CDS             complement(<1..292)
                     /locus_tag="VM910_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65289.1"
                     /translation="MAVMGIPDGSGRLVPRKASARSRVSNGGHILPDVDGRSAVARRY
                     RDIAAQIIADMGGASQCAEARLQLIRRFAAAAVLAEQMEARLANGQSINITDH"
     gene            complement(605..1027)
                     /locus_tag="VM910_22310"
     CDS             complement(605..1027)
                     /locus_tag="VM910_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65290.1"
                     /translation="MAKKRSRLRLVSEHDPASVFDDLDALRQAQAAPAGSAFQGQRRQ
                     RSSETFARIPHDRGFELNRRNLSGAAWALLIVLDRLIFEGHGKNPVKLTHRWLKAAGL
                     SRFAAMRALRLLESAGVVSVERRRGRAPLVAHRWFPVA"
     gene            complement(1020..2627)
                     /locus_tag="VM910_22315"
     CDS             complement(1020..2627)
                     /locus_tag="VM910_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI65291.1"
                     /translation="MVAFASNAELACFLALGWPFPTNVLDPYVENIVTINGNTAVWPP
                     ADEKKRKGRPGLLDSLKLHDLPARPQEHKDRMRDMILDNEDYTPEQRREIQDYNQEDV
                     DDTIALLDTLGQPVDDINHALFRGRYMAAVAREERVGLPDDTDYLTQLLDVWEALQLY
                     YIQQHDEFHLYDGTHFIEARLWNLVEEKGWDWPRTATGRYQLKISTIGRQATRYPELE
                     SLARLRDTITELRINKLGNTVGPDGFSRMPLLPFWTVTGRNQPSAKDKMFLLGLPTWL
                     HGILKPPRGMALVELDFVAEEFAIAAGLSGDPAMIADYQNGDVHWQFGLRAGLAAPDT
                     AVDSRIRDLCKVVVHGSNYGMTPYGIAVKTRKSLDWAREIHTHHRHAYPVFHQWLGDV
                     VAQARFDEVITSPFGWRQIVTANTRSRSLMNFMAQAGGSDVMRLATIAATECGITIAA
                     PVHDAFWILAPLDELETTITRMTEIMTEAGRLVAGIPIRVKVEAVVRWPQCLGDTRAA
                     DAKGQAMWCEVRELVRNGGLRRQVSYG"
     gene            complement(2818..3405)
                     /locus_tag="VM910_22320"
     CDS             complement(2818..3405)
                     /locus_tag="VM910_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65292.1"
                     /translation="MDDNTKPPQGDPADIFNDLAALRKASKLTVKRQIVLVNVPVGRP
                     ANNVYFRTHKDIKLEDATVIRDEEGGRKAFYFIVPAMRDHPKLAPRLRYVTIRLVCTW
                     PGDGILLWPVPARADFAAWRSEQAAATLAETKWTQLVWNSERADFDVETAENIDREPT
                     WPKESFEPLLKLGFADRIVDNKDHYYVRRLRGVID"
     gene            complement(3603..3980)
                     /locus_tag="VM910_22325"
     CDS             complement(3603..3980)
                     /locus_tag="VM910_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65293.1"
                     /translation="MSARERLPNRRPSTVFEIEVHGMNYTVSFSRFADGRAGDEIMLI
                     KADGTAWGRSEQLRPMAAAVRAARIDAEAAAAWRKERENPKADEAKELARLAERRQQR
                     SRRAAAWAVASPDHVSNRRRQLA"
     gene            complement(3977..4366)
                     /locus_tag="VM910_22330"
     CDS             complement(3977..4366)
                     /locus_tag="VM910_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65294.1"
                     /translation="MSRRRARPEDAIQHAVCEHLNIRGVPGLVWFHVPQGNKLGGKFN
                     VKGTAIRGAINKGLGVKRGVSDLIFLQAAKFFALELKAEGRTPTAEQLAFIADVNAAG
                     GFACWCAGLDRALACLEQWGLLKGRAS"
     gene            complement(4363..4530)
                     /locus_tag="VM910_22335"
     CDS             complement(4363..4530)
                     /locus_tag="VM910_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65295.1"
                     /translation="MNTKFEHRGYAVVVPAPRYADLFSRYQHNDKWHCHGDEAPPDPL
                     DIPACLRRVAP"
     gene            complement(4527..4811)
                     /locus_tag="VM910_22340"
     CDS             complement(4527..4811)
                     /locus_tag="VM910_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65296.1"
                     /translation="MSTDDDNKLVTVDAAVALIRERTGIPIPKSRLHKDSASGVAPRP
                     DAIYGRRYLWRPERILAYARTLIKPHSECDGDNSQSLGNAAKRVVQKAKP"
     gene            complement(4869..5420)
                     /locus_tag="VM910_22345"
     CDS             complement(4869..5420)
                     /locus_tag="VM910_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65297.1"
                     /translation="MKTKTGEVRLTEPGRRETKEHAEAHQDALSGFIRVARYQRNEFL
                     RYALAGDWAPLAKYIRDGGRVTPRMRPFLADVLDRKRPRPRVKISRWATRRRNGEIAG
                     FIFDARFRGEKEKEYSKAAEKKFGLTWRHIQKILAEEELRSETDKILQNDWQGIFLLN
                     PPSDNGGIKYRLSDMALSPHTLP"
     gene            complement(5417..6598)
                     /locus_tag="VM910_22350"
     CDS             complement(5417..6598)
                     /locus_tag="VM910_22350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI65298.1"
                     /translation="MRLRLTPASVRNAQADADADRTIFWDTKTPGFGLQVTAAGHKSF
                     VIQYRAKGRSRRMAIDGVLGLEAARKRAKVLLGEVAHDRDPLGERRAARARKQDTFQA
                     VAESYFAREGKKLRTIEDRRRAMARLVYPAMGARPIADILRSDIVRLLDRIEDENGPV
                     MADHTLAYVRKVMNWHASRSDEFRSPIVRGMARTSGKERARARILTDDELRAVWKAAE
                     ATAGPFGAFIKFLLLTGARRHEASNATWDELKGGVWLLPAARNKVKQDLARPLSSAAQ
                     NLVGGLPKIGRYVFTFGGVKPLSGFAKPKARLDAASGAKGWILHDLRRTARSLLSRAG
                     IAPDVAEMCLGHVLTGVRGTYDRHAYHEEKKHAFEALAAQIARIVDPRDNIVALRGTL
                     S"
     gene            complement(6794..7453)
                     /gene="gmk"
                     /locus_tag="VM910_22355"
     CDS             complement(6794..7453)
                     /gene="gmk"
                     /locus_tag="VM910_22355"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVI65299.1"
                     /translation="MTASGGQQQSDAVARRGLMLVLSSPSGAGKTTLSRRLLDADPGV
                     ELSVSVTTRKQRPGEVDGRDYHFIDAARFEAMIKGDELLEWAHVFGHRYGTPRAPVEA
                     ALVRGHDVLFDIDWQGTQQLREKAHRDLVSIFVLPPSMSDLERRLRQRAQDSDEVIHA
                     RMATATDEMSHWAEYDYVVINSDVDHAFAQVQSILAAERLKRERRTGLSEFIRRLQAQ
                     L"
     gene            complement(7458..>7628)
                     /locus_tag="VM910_22360"
     CDS             complement(7458..>7628)
                     /locus_tag="VM910_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HVI65300.1"
                     /translation="IAGGGPIGRRLDFLAQELNREANTLCSKSNDVELTNIGLELKSV
                     VEQFREQVQNLE"
BASE COUNT         1380 a         2367 c         2307 g         1574 t
ORIGIN      
        1 catgatctgt gatgttgatc gactggccat ttgccaatcg tgcttccatc tgctcggcaa
       61 gaacggctgc agccgcgaag cggcgaatga gttgaaggcg tgcttcggcg cattgcgacg
      121 caccgcccat gtcggcgatg atttgcgcgg caatatcgcg gtagcgccta gcaacagcgg
      181 agcggccgtc cacgtcggga aggatatggc ctccgttaga tactcggctg cgggctgagg
      241 ccttgcgcgg aacaaggcgg ccgcttccgt cggggattcc cattacggcc attccgtctg
      301 ctccattccg cagtttctgg tccgcttatt ggagtaacct tggacagtat acctgttcgc
      361 gtcaaaagat ataacggagc ttcgtgacat cgctgtgcga gacaccgcgt ttcaccttgc
      421 tcaccgagct cgcctccgta cctgcctcat tcctggttaa gaagagaaca gaagagaaga
      481 gaagatagag agaaagagaa taggaacggc tgacaggtgt gcggatgtgc aacaggtacc
      541 tgtgcggatg tgcaacaggt acctgtgcgg tgtgcaacag gtttattcgg atgtgcaaca
      601 cgtcctacgc aacagggaac cagcgatggg ccactagcgg cgctcgtccg cgtcgtcgtt
      661 cgacggagac gacgcctgca ctctccaata agcgcaaggc ccgcatcgcg gcgaagcgcg
      721 aaagcccagc agctttcagc caccgatggg ttagcttgac cgggttcttg ccgtggcctt
      781 caaaaatgag tctatcaagc acgattaaga gcgcccaagc cgccccgctc aggttccgac
      841 ggttgagttc aaatcctcga tcatgcggga tgcgggcaaa cgtttcacta ctacgctgcc
      901 ggcgctgtcc ctgaaaagca gacccagccg gggccgcctg agcttggcgc agggcatcga
      961 gatcgtcgaa gacgctggcc ggatcgtgct cgcttacgag ccgcagccgg ctccgtttct
     1021 tagccatagg acacctgtct ccgtaagcca ccgttgcgga cgagctcgcg cacctcgcac
     1081 cacatagcct gccccttggc gtcggccgca cgggtatcgc cgaggcactg cggccagcgc
     1141 accaccgctt caactttcac ccggatcgga atgccggcta ccagccgtcc ggcctcggtc
     1201 atgatctcgg tcatgcgcgt gatcgtggtt tcgagttcgt cgagcggcgc caaaatccag
     1261 aacgcgtcgt gcacgggtgc agcgatggtg atcccgcatt cggtggccgc gatggtggcg
     1321 aggcgcatca cgtcgctacc accggcctgc gccatgaaat tcattaaact gcggctgcgg
     1381 gtgttggcgg tcacgatctg gcgccaacca aacgggctcg tgatgacttc gtcaaagcgc
     1441 gcctgtgcga cgacgtcacc gagccattga tggaagaccg gataggcgtg tcggtggtga
     1501 gtgtgtattt cacgcgccca atccaaggat ttcctcgtct ttacggcgat gccgtaaggt
     1561 gtcatgccgt aattggaccc gtgaacaacg actttgcaga ggtcacggat acgactgtcg
     1621 acggccgtgt caggtgcagc gagcccggct cgtaatccaa attgccagtg cacgtcaccg
     1681 ttctgatagt ccgcgatcat tgccggatca ccacttaatc cggccgcgat ggcgaactct
     1741 tcggccacga aatcaagctc aaccagcgcc atgccgcgcg gcggcttaag tatcccgtgc
     1801 aaccacgtcg gcaaccctag aagaaacatc ttatccttgg ccgagggttg atttctgcca
     1861 gtcacggtcc aaaacggtag caaaggcatg cggctgaaac cgtccggccc gaccgtattg
     1921 cccaacttgt tgatgcgcaa ttccgtgatt gtgtcgcgca ggcgagcaag cgattcgagc
     1981 tcaggataac gcgtcgcctg tctgccgata gttgagatct tgagttggta acgtccggtc
     2041 gctgtccgcg gccagtccca acctttctct tcgacaagat tccagagccg cgcttcgatg
     2101 aagtgtgtgc catcatacaa atgaaattcg tcgtgctgct ggatgtagta cagttgcagc
     2161 gcttcccata cgtctaggag ttgggtgagg taatcggtat cgtcgggcag cccgacgcgt
     2221 tcctcgcggg ccaccgcggc catatagcgg ccgcgaaaca atgcgtgatt gatgtcatcg
     2281 acgggctgcc ctagggtgtc gagcagcgcg atcgtgtcgt caacgtcttc ctggttgtaa
     2341 tcctgaattt cgcggcgttg ctctggggtg tagtcctcgt tgtcgaggat catgtcgcgc
     2401 attcggtcct tatgctcttg tggcctcgct ggtaaatcgt gcagcttaag cgagtcgagc
     2461 agtccaggcc gccccttgcg cttcttttca tcggctggcg gccaaaccgc ggtattgccg
     2521 ttgatggtga cgatgttctc gacatagggg tcgagcacgt tggtaggaaa cggccagccg
     2581 agcgcgagga agcacgccaa ttccgcgttg ctggcgaacg ccaccatcac gctgtcgggg
     2641 ccggtatcga acggaccgtg ggtcaggcgt aggagctcat cccgccacag cgaaacgctc
     2701 gcgccggagt gctcctcgcg cgcatacata cagacaggta tcgggagatg gttggcgtcc
     2761 tcgcgaaact cgaagtcgac gtgccagatc gatcgaaacc gctgacgcca atcaccatta
     2821 gtcgatgacg cccctgagcc ggcggacata atagtggtcc ttgttatcta cgatacggtc
     2881 cgcaaagccg agcttgagca gcggctcgaa gctttccttt ggccaggtcg gttctctgtc
     2941 gatgttctct gccgtctcga cgtcgaagtc ggcgcgttcg gagttccaca ccagctgcgt
     3001 ccacttggtc tcggccagcg ttgccgcagc ctgctccgag cgccacgctg cgaagtccgc
     3061 tcgcgccggc accggccaca gcaaaatccc gtcgccgggc cacgtgcaca ccaatctgat
     3121 agtcacgtac cgcaagcgcg gtgcgagctt gggatgatcg cgcatggctg ggacgatgaa
     3181 atagaaggct ttgcgcccgc cttcctcgtc gcgaatcacc gtcgcatctt cgagcttgat
     3241 gtctttgtgg gtgcggaagt agacgttgtt cgccggcctg ccgaccggca cgttcaccag
     3301 gacgatctgg cgcttgacag taagcttgga cgccttccgc aatgctgcga ggtcgttgaa
     3361 gatgtccgca ggatcccctt gcgggggttt tgtgttatca tccatgtgtt cggcccctcc
     3421 tttcgggccg gacgcgacct gctaactgcg cgggtctccg gcgacgggcc ggtcgcgaac
     3481 agcgcccgtc accaaagcgt tggacgccgc cgctccttga ccgagcggcg gtgtcgcttg
     3541 ttggtgagct caagggggat ggcggctcgg cacgacgagg cccccggctt tcggtggcgc
     3601 gctcacgcaa gctgccgacg tcggtttgaa acgtgatccg gacttgcaac ggcccatgct
     3661 gcggccctgc ggcttcgctg ctgacgccgc tcggcaagtc gcgccagctc tttggcttca
     3721 tcggccttcg gattctcgcg ctccttgcgc cacgccgctg cagcctcggc gtcgatcctc
     3781 gccgcgcgaa cggcggcggc catgggccgc aactgctcgg acctgcccca tgccgtgccg
     3841 tccgccttga tcagcatgat ctcgtcgccg gcgcggccgt cggcgaatct tgagaagctg
     3901 acggtgtaat tcatgccgtg cacctcgatc tcgaacacgg tcgagggacg tcgattgggc
     3961 aatcgctcgc gcgcgctcat gaggcacgcc ccttcagcaa gccccactgc tctaagcaag
     4021 cgagcgcgcg gtcgagaccg gcgcaccagc acgcgaatcc gccagcggca ttgacgtcgg
     4081 cgatgaaggc aagctgctcg gcggtcggcg tccgaccttc tgcctttagc tccagcgcga
     4141 aaaattttgc ggcttgcagg aagatgaggt cactgacgcc gcgcttgacg ccgagcccct
     4201 tgttgatcgc acctcggatg gcggtgccct tgacgttgaa cttgccgccg agcttgttcc
     4261 cttgtgggac atgaaaccaa acgagccctg gaacgccgcg aatattgaga tgttcgcaga
     4321 ccgcgtgttg aatcgcatct tcggggcgag cgcgacggcg gctcatggcg cgacccttcg
     4381 caagcatgcg gggatgtcga gtggatcggg cggcgcttcg tcaccgtggc aatgccactt
     4441 gtcgttgtgc tggtagcgcg agaacagatc ggcgtagcgc ggcgcgggga ccaccacggc
     4501 gtagcctcgg tgttcgaatt tcgtgttcac ggcttcgcct tctgaacgac gcgcttcgcg
     4561 gcattgccaa gcgattgcga gttgtcgccg tcacattctg agtgaggctt aatcagggtt
     4621 cgcgcgtagg cgagaatccg ctccggtcgc cacaggtagc ggcggccata gatcgcgtcc
     4681 ggtcgcggcg cgaccccgct ggcgctgtcc ttgtgaaggc gtgattttgg aatcggaatg
     4741 ccggtccgct cgcggatcaa cgccacggcg gcgtcaaccg tcaccagctt gttgtcgtcg
     4801 tcggtgctca ttgcacttcg tcctctaatt caacgaagca caatatgacg gtgatgctgg
     4861 tgtgaaagct acggcaaagt atgtggactt agtgccatgt cactaagtcg gtacttaatg
     4921 ccaccattat cggagggggg gttcaaaagg aagatgcctt gccagtcgtt ctgaagtatt
     4981 ttgtctgttt cgctgcgcag ttcctcttcc gccaggattt tttgtatgtg tcgccacgtt
     5041 agcccaaact tcttttcggc tgccttgctg tactcttttt ctttctcacc acggaaacgg
     5101 gcatcaaaga tgaagcctgc aatctcacca tttcttcgcc tcgttgccca cctgcttatt
     5161 ttcacacgcg ggcgcggtct cttcctgtcc agaacatcgg cgagaaacgg gcgcattcgg
     5221 ggggtgacgc gaccaccgtc gcggatgtac tttgccaacg gtgcccaatc gccagcgagc
     5281 gcgtagcgca agaattcgtt gcgttgatat cgtgcgacgc ggataaaacc agacaaagcg
     5341 tcttggtggg cttccgcatg ttcttttgtt tcgcggcgcc caggctccgt cagcctcacc
     5401 tctcccgtct tggttttcat gacaacgtac cccgcaaggc cacaatattg tcccgcggat
     5461 cgacgatgcg agcgatctgc gccgcgagcg cctcgaaggc gtgcttcttc tcctcatgat
     5521 acgcatgtcg gtcatatgtc ccgcgcacgc ccgtcaacac atgccctaag cacatttcag
     5581 ccacgtcggg cgcgatgccg gcgcggctta gtagcgagcg cgccgtgcgc cgaagatcgt
     5641 gcaggatcca gcccttggcc cccgaggcag cgtcgaggcg cgccttgggt ttggcgaagc
     5701 cgctaagggg tttcacgccg ccgaaggtga agacatagcg cccgatcttc ggtagaccac
     5761 ccacaaggtt ctgcgcggcg ctggagagcg gtctggcgag atcctgcttc accttgttgc
     5821 gcgcggccgg cagcaaccag acgccgccct tgagctcgtc ccaagtcgcg ttggatgctt
     5881 cgtgcctgcg cgcgccggtg agcaacagaa acttgatgaa cgccccgaac ggtcccgcgg
     5941 tggcctctgc ggccttccag acggccctaa gctcgtcgtc ggtcaggatg cgcgctcgag
     6001 ctcgctcttt cccgctggtc cgcgccatgc cacgcacaat cggcgagcga aattcatcag
     6061 agcggctggc gtgccagttc ataaccttgc ggacgtaggc cagggtgtgg tccgccatca
     6121 ccggtccgtt ttcgtcctcg atgcggtcga gcagccgcac gatgtcgctg cggagaatgt
     6181 ccgcgatcgg cctagcgccc atggccggat agaccagcct ggccatggcc cgccgccggt
     6241 cttcgatcgt gcgcagtttt ttgccttcgc gcgcgaaata gctctcggca acggcttgga
     6301 acgtgtcctg tttgcgagct cgcgcggcgc ggcgttcgcc caacggatcg cggtcgtgcg
     6361 ccacctcgcc cagcaagact ttcgcccgct tgcgcgcggc ttccaggccg agcacgccgt
     6421 cgatcgccat gcgccggctg cgccccttgg ctcgatactg gatcacgaag ctcttgtgcc
     6481 ccgcggccgt cacctgcaac ccgaagccgg gggtcttggt gtcccagaaa attgtcctgt
     6541 cagcgtcggc gtcggcttgc gcgttgcgca cgctggccgg cgttaatcta agccgcatgg
     6601 ccgaccctct tcacgagacg tcaaagtgaa cgtcgggtaa gcgtcgggta agcgtcagtg
     6661 caaaatacgt catgaaacga aataaaacaa gatgtaacaa taaatgcccg tcgtatcggc
     6721 atttctgttg cataccagcc acaggggtct aacgaaaaaa tatgcaccga aacgactatt
     6781 ccaggattcg tcgtcacagc tgcgcctgca gccggcggat gaactcggag agtccggtcc
     6841 gccgctcgcg cttgaggcgc tcggccgcca ggattgattg cacctgcgcg aaggcgtgat
     6901 cgacgtcgct attaatcacg acgtagtcgt actccgccca gtgactcatt tcgtcggtcg
     6961 ccgtggccat gcgagcgtgg atcacctcat cggaatcctg tgctcgctgc cgcagccgcc
     7021 gctcgaggtc ggacattgat ggcggcagca cgaagatgct gacgagatcg cggtgcgcct
     7081 tttcgcgaag ctgctgcgtc ccctgccagt cgatgtcgaa caaaacgtcg tgcccgcgca
     7141 cgagcgcggc ctccaccggc gcgcgcggcg tgccgtagcg atggccgaat acgtgcgccc
     7201 actccagcag ctcgtcgccc ttgatcatgg cctcgaatcg ggcggcgtcg atgaaatgat
     7261 agtcgcggcc gtcgacctcg cccggccttt gcttgcgcgt cgtcaccgag accgaaagct
     7321 ccacgccggg atcggcgtcg agcagcctgc gcgacaacgt cgtcttccct gcacccgaag
     7381 gcgaggacag cactagcatg agcccgcggc gggcgacggc gtcgctctgt tgctgacccc
     7441 cgcttgccgt catcagctca ttccaaattc tgcacttgct cgcggaactg ctcgaccacg
     7501 ctcttgagct ccagcccgat gttggtcaac tccacgtcgt ttgacttgga gcacagcgtg
     7561 ttcgcctcgc gattgagctc ctgcgcgagg aaatcgagtc ggcggccgat tgggccccca
     7621 ccggcaat
//
LOCUS       DATLJT010000971         8235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_295468,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000971 DATLJT010000000
VERSION     DATLJT010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295468"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VM910_22365"
     CDS             <1..402
                     /locus_tag="VM910_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HVI65301.1"
                     /translation="HQGNIPDQEFGWGRSCDEFTKPATLLGPHSAPLGMRFYTGNMFP
                     SEYRNAIFVARHGSWNRSKKFGGDIVVVSLNENGTVKSVDPFITGFIENNNYIGRPAD
                     LMVLKDGSMLISDDYNGAVYRVTYDGASVAR"
     gene            complement(520..828)
                     /locus_tag="VM910_22370"
     CDS             complement(520..828)
                     /locus_tag="VM910_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HVI65302.1"
                     /translation="MTVRTLVTSAAIALWFGASPPATMAQSDDFVNGQVMKIDEPAGK
                     ITIKHGPLRKFDMNENMTMVFRAGDPAMLKAVKPGDRVRFVPDRVNGQFTVTKMEKVR
                     "
     gene            complement(859..1356)
                     /locus_tag="VM910_22375"
     CDS             complement(859..1356)
                     /locus_tag="VM910_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HVI65303.1"
                     /translation="MIIYPLRLIAGALLLAFVEPSTIRTDLTAVAYAHESHQSFAAGE
                     PGDPKKLARTIKVVMREDGKKMAFEPARITVRKGEQIRLVLENGGTDDHEFVLATVKE
                     NRKHAALMKKFPDMEHDDPNAKRLAPGAHGEILWKFTKAGTFEFACLIPGHREAGMLG
                     KVVVK"
     gene            complement(1369..2763)
                     /locus_tag="VM910_22380"
     CDS             complement(1369..2763)
                     /locus_tag="VM910_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HVI65304.1"
                     /translation="MISRRGFLGTAAVLASATAVSGRVQAAGIPEAPSTKTTTMQPPI
                     FPTSGPDYQPVVTLNGWTLPWRMNGDWKEFHLVAEPVVREFAPGMKVNLWGYNGQAPG
                     PTIEAVEGDKVRIFVTNKLPEHTTVHWHGMLLPNGMDGVGGVTQPHIEPGKTFVYEFE
                     IKHSGTFMYHPHSDEMVQMGMGMMGMFVVHPRDPRFRRVDRDFVFIMSAYLIDPGTHL
                     PKVAEMTDFNMWTWNSRVFPGIDPMPVRLGDRVRVRMGNLTMTNHPIHLHGHHFSVSC
                     TDGGWVPESAQWPESTVDVPVGTIRAFDMVADNPGDWAFHCHKTHHAMNAMGHNVRNF
                     VGVQKRDVARQIRKLVPDYMPMGSEGMAVMGEMEMPMPDNTLPMMTGFGQFGPIEMGG
                     MFTIMKIREELATNDYRDPGPYKNPAGTVAYEFQGTPPSEPARPIDNPPGRGGAAEPN
                     RWKPNARPSSSHQH"
     gene            complement(2778..4157)
                     /locus_tag="VM910_22385"
     CDS             complement(2778..4157)
                     /locus_tag="VM910_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVI65305.1"
                     /translation="MLLSGCTWFSPDAGMGVVATIAQQELKKDAAAIRSPEEAEVAGA
                     TVRRLLGRTLTADAAVQTALLNNRGLQAAYDELAIADADRVGQSLPPNPTFSWRRIQA
                     GLALESEMQLVADIIALATLPARSELAAERFHQAQLRAAEETLRVAHEARRAYYQAVA
                     ARELVHFLTESRSAAEAATQLAARLGETGAVNKLDQAREQVFYADLTAQLATARQREA
                     SEREALIRAMGLWGTSLDFKIPNALPPLPHRVQTLAGIEVDAVGRRVDLQIARIELDV
                     LAKSYGLTQASRFINILDAGYADKLEKRVDERVLMRGFDVRLQIPLFDFGEVRVRQAE
                     ATYMQAVNRLLELAVNVRSEARDAYRVYGSTYDIAGHYQREVLPLRKIISDETLLRYN
                     AMLIDVFSLLAEARQRIAATITAIEAKRDFWLATVDLKAAIAGGGRSGNRNERIDPMA
                     ASAGKADAR"
     gene            complement(4257..4553)
                     /locus_tag="VM910_22390"
     CDS             complement(4257..4553)
                     /locus_tag="VM910_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65306.1"
                     /translation="MSVHLRTFDAASRPAVTRAFRLELVTLAALAGLVLLLVGCTDAP
                     RWPVAGPDPSDPGVRVPRVGYRSTIAPYNSQRPVEPGPWGEQNQRVAPQPKSGQ"
     gene            complement(4627..5031)
                     /locus_tag="VM910_22395"
     CDS             complement(4627..5031)
                     /locus_tag="VM910_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65307.1"
                     /translation="MTKTFRARAAIVLAVLYALCILAPSVAFAFSDNPAVAHCLTEGH
                     VGPHDHGGKVHAHADGTAHQHHDDGTAPQPAGDDGKAPVANCCGLFSVVAIPDQPDIN
                     LGLNIPASVVVPALAEALSGRGPERINRPPIA"
     gene            complement(5240..5671)
                     /locus_tag="VM910_22400"
     CDS             complement(5240..5671)
                     /locus_tag="VM910_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65308.1"
                     /translation="MKHPLTRIWLYTACLFLTFGSDPGWAQHPGARSTPSHGAAIPQA
                     GPANAMVEFENESIVVVRIRMAPYEKTPMHDIASERLVVWLTDVYLRDTHADGSTNDI
                     HRRAGDVDWVPIQRHAGENLAHESLEFLAIVPKTRGAAAVK"
     gene            5766..6191
                     /locus_tag="VM910_22405"
     CDS             5766..6191
                     /locus_tag="VM910_22405"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVI65309.1"
                     /translation="MPGMSIGEVAKKMGLRSSAIRYYERLGLIPKAPRLSGRRRYDDR
                     VLERLAMVRFAKHVGFSIAEIKVLLRGVDGRPPPERWRKLAAHKVAQVDAFVAQARNI
                     RKMLSETLDFQCPRLVERGRALHSTTARPALRLKRNTRS"
     gene            complement(6316..7755)
                     /locus_tag="VM910_22410"
     CDS             complement(6316..7755)
                     /locus_tag="VM910_22410"
                     /inference="COORDINATES: protein motif:HMM:NF022821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HVI65310.1"
                     /translation="MDEGSITMATGVVVVYFSRFGKPLQTHHKVVLDLGAKSIAKIKG
                     YDFSGHYESGCDYSDPLFFVPGDTLLLDEASRLGIRSANDLYGGVVPHLFVKTKAITH
                     GLVDRDAERPQGWCTAFAERVREIVLPGYTVFSNRDARVAARRMLRRGPLRLKKPLSA
                     SGRDQTLVTSLNELDADLENITADEIATYGLVLEENLRQVRTLSIGEVTIGSLRISYH
                     GTQRTVRDNEGRPVYGGSDLVCVRGGWKSLDALPMPSEVRAAVAAARQYDAAAEEFSG
                     FIASRRNYDVAQGIGADDWPQSGVLEPSWRVGGASSAELAALATFARDPSLKIVRASH
                     FEEYGHSRRAPAHAIVEFQGDDPEAGPLLRYTIVTPQDQQLRKKICGPCVGAWRGLPA
                     SVLSSDADSRVIRFRSRTDASPRSQRGKGPIGHSGPDYSLVPDLSKYECPESEDDYRH
                     RMVINAVALVFVSLLSLAGFWLVNVIAHS"
     gene            complement(8011..>8235)
                     /locus_tag="VM910_22415"
     CDS             complement(8011..>8235)
                     /locus_tag="VM910_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65311.1"
                     /translation="LFFDAKRQRVYLSCGDGYVDVFEAQGDAYGRSAHIPTISGARTA
                     LFVPELDRFFVAARANPGEPAALWVFRPIP"
BASE COUNT         1539 a         2505 c         2527 g         1664 t
ORIGIN      
        1 caccagggca acatccccga ccaggaattc ggctggggac gctcgtgcga cgaattcacg
       61 aaaccagcca cgctattggg gccacattca gccccgctcg gcatgcggtt ctacaccggc
      121 aacatgttcc cgtcggaata ccgcaatgcc attttcgtcg cgcgccatgg ctcgtggaat
      181 cgatccaaga agttcggcgg cgacatcgtc gtcgtgtccc tcaacgagaa cggtacggtg
      241 aaatccgtcg atccgttcat cacgggcttc attgaaaaca acaactatat cggacgcccg
      301 gctgacctca tggtcctcaa ggacggctcg atgctgatct ccgacgacta caacggcgcg
      361 gtctatcgag tcacgtacga cggcgcgagc gtggccagat agtttcttga agtgaccttt
      421 tgctcgaggg cggcgtctgt cgccctcgac gcatggcatc ctcagcgttc acttcgacta
      481 ctgatgcgcc atccattaga cttcttaacc ctcccctagt catcttacct tttccatctt
      541 tgtcaccgtg aactggccgt tgacccggtc cggcacgaac cggacacggt cgcccggctt
      601 gactgctttc agcatggcgg gatcgccggc gcgaaatacc atggtcatgt tctcgttcat
      661 gtcgaatttc ctgagcgggc cgtgcttgat cgtgatcttg cccgcgggct cgtcaatctt
      721 catgacttga ccgttcacaa agtcatccga ttgcgccatc gtcgccggcg ggctcgcgcc
      781 gaaccacagt gctatcgccg cagaggtgac gagagtcctt actgtcatga tggcctcctt
      841 tgcaccgggt gattgcgctc acttgacgac aaccttgccc agcattcccg cctcgcggtg
      901 tcccgggatc aggcaggcaa attcgaatgt gccggctttt gtgaatttcc acaggatttc
      961 gccatgggcg ccgggcgcaa gacgttttgc attcgggtca tcgtgctcca tatcgggaaa
     1021 cttcttcatc aatgcggcgt gcttacggtt ttcctttaca gtcgcgagaa cgaactcgtg
     1081 atcgtcggtg ccgccgtttt ccaaaacgag gcggatttgt tcgcctttgc gcactgtgat
     1141 ccgggcgggc tcgaaagcca ttttcttccc atcttcgcgc atgacgacct tgatagtgcg
     1201 ggccagcttt ttggggtcac ccggctcgcc ggcggcaaag ctctggtggc tttcgtgggc
     1261 atacgcgaca gcggtcaggt cggtacggat ggtgctgggc tccacgaagg cgagaagcag
     1321 tgcaccggcg atcaatcgaa ggggatagat gatcatgagg tcctcacgtt agtgttgatg
     1381 cgacgacgaa ggtcttgcgt tcggtttcca gcggttcggt tccgccgccc ctccccggcc
     1441 tggcggattg tcgatagggc gcgcgggttc actaggcggg gtgccttgga actcgtatgc
     1501 gacggtgccc gcgggatttt tgtatgggcc ggggtcccgg taatcgttgg tcgcgagctc
     1561 ttcgcgaatt ttcatgatgg taaacatgcc gcccatttcg atcggtccga actgtccgaa
     1621 cccggtcatc atcggcagcg tgttgtcggg catcggcatt tccatctcgc ccatcacagc
     1681 catgccctcc gagcccatag gcatgtagtc gggcacgagc ttgcggattt gtcgcgcgac
     1741 atcgcgcttt tgcacgccga caaaattcct cacgttgtga cccatggcgt tcatggcgtg
     1801 atgcgtcttg tgacagtgga aagcccagtc tcccgggttg tcggcaacca tgtcaaaggc
     1861 tcggatggta ccgaccggga cgtcgacggt actctccggc cattgcgcac tctccggcac
     1921 ccaaccaccg tccgtgcaac tcaccgaaaa atggtggcca tgcaaatgga tcggatggtt
     1981 ggtcatggtg agattgccca tccgcacgcg cacgcggtcg ccgagccgca ccggcatcgg
     2041 atcgatgccg ggaaacacgc ggctgttcca cgtccacatg ttgaaatcgg tcatctccgc
     2101 gaccttcggc agatgcgtcc cgggatcgat caggtacgcg ctcataatga atacgaagtc
     2161 gcgatcgacg cggcgaaatc tcgggtcgcg cgggtgaacg acgaacatgc ccatcatgcc
     2221 catccccatc tgcaccattt cgtccgaatg cgggtgatac atgaaggtcc cgctgtgctt
     2281 gatctcgaac tcgtagacga aggtcttacc cggctcgatg tgcggctgcg tcacgccgcc
     2341 gacgccgtcc atcccgttcg gcagcagcat cccgtgccaa tgcacggtgg tgtgctccgg
     2401 cagcttgttg gtgacgaaga tgcgcacctt gtcgccttcg accgcctcga tcgtcggtcc
     2461 tggtgcctgt ccgttgtaac cccacaaatt caccttcatg cccggcgcga actcgcgcac
     2521 caccggctct gcaacgaggt gaaactcctt ccagtcgccg ttcatgcgcc acggcaaagt
     2581 ccagccgttg agcgtgacga ccggctgata gtcgggaccg ctcgtcggaa agatcggcgg
     2641 ttgcatggtg gttgtcttgg tgctcggcgc ctcgggaatg ccagccgcct gcacgcgccc
     2701 actgacagcg gtagcacttg ccaacaccgc ggcggtgccg agaaagcctc tgcgcgaaat
     2761 catggtcttc tccttgatca acgtgcgtcc gccttgccgg cggatgccgc catggggtct
     2821 attcgttcat ttctgttacc cgacctgccg ccgccggcga tggccgcctt gaggtccacg
     2881 gttgcgagcc agaaatcgcg cttcgcctcg atcgccgtga tcgtcgccgc aatgcgctgg
     2941 cgcgcctccg cgagcagtga aaacacgtca attagcatcg cgttatagcg aagcagcgtt
     3001 tcgtcggaga tgatcttgcg cagcggcaag acctcgcgct gataatggcc tgctatgtca
     3061 taggtcgagc cgtagacgcg gtacgcatca cgtgcctctg agcgcacatt aaccgccaat
     3121 tcgagcagcc gattaacggc ctgcatatag gtcgcctcgg cctggcggac gcggacctcg
     3181 ccgaagtcga acagcgggat ttgtaatcgg acatcgaagc cgcgcatcag aacgcgttcg
     3241 tcgactcgct tttccagctt atcggcgtag ccggcgtcga gaatattgat gaatcggctg
     3301 gcttgggtga ggccgtagga tttcgcgagc acgtcgagct cgatacgggc gatctgcagg
     3361 tccacgcgcc ggccgaccgc gtccacctct atccctgcca acgtctgaac acgatgtggc
     3421 agcggcggaa gcgcatttgg aattttgaag tcgagacttg tgccccacag gcccatcgcg
     3481 cgaatgagcg cctcgcgctc gcttgcctcc cgctggcgcg cggtggcgag ctgtgcggtc
     3541 aagtcggcat agaatacctg ctcgcgggcc tgatcgagct tgttcaccgc cccggtttcg
     3601 ccgagccgcg ctgctaactg ggttgcggct tcggccgccg accgcgattc cgtcaagaag
     3661 tggacgagct cgcgcgctgc aacggcttga tagtaagcgc gccgggcttc atgggcgacg
     3721 cggagcgtct cttcggcggc gcgcagctgc gcttgatgaa agcgttccgc ggcaagctcg
     3781 gatcgcgccg gcagggtcgc aagtgcgatg atatcggcga cgagttgcat ctcgctctcg
     3841 agcgccagcc ccgcctggat gcgtcgccat gaaaacgttg gattcggcgg aaggctttgt
     3901 ccgactcggt cagcatcggc gatggcgagc tcatcgtatg ccgcctgcag accgcggttg
     3961 ttgagcagcg cggtctggac cgccgcgtcc gcggttaggg tgcggccgag aaggcggcga
     4021 acggtggcgc cggcgacttc cgcctcctcg ggcgagcgga tcgcggccgc gtccttcttc
     4081 agctcctgct gtgcgatcgt ggcgacgact cccatacccg catccggcga aaaccaggtg
     4141 caaccggaaa gcagcatcgc cgaggcgagg cccgacctcg cgcggaatag tcggcgcagc
     4201 gagtgcggcc gatgcgggaa agcaagcgaa cgaagttccc acaccatcgg gcgctcctac
     4261 tgcccggatt tgggttgtgg agcgacgcgc tggttctgct cgccccaagg cccgggctcg
     4321 accggccgct ggctattgta aggcgcaatc gtcgagcgat agccaacccg cggcacgcga
     4381 acccctggat cggatggatc ggggcccgcc accggccagc gtggcgcgtc ggtgcacccc
     4441 acgagcagca gaacgagccc cgccaacgcc gcgagcgtga ctaactcgag ccggaaagcc
     4501 cgagtgaccg ccggtctgct agcggcatcg aacgtccgaa ggtgcacgga catggaatcc
     4561 tcgactgtcg attgcgggcg ggcgtgcgcg accgttcagc gcacgcgccg gcttcaactg
     4621 cgacagtcag gcgatgggag gccggttgat gcgttccggg ccgcgcccgc tcagggcctc
     4681 ggccagagct ggcacgacca cagaagctgg gatgttaagt ccgagattga tatccggctg
     4741 gtcgggaatt gccacgacgg aaaacaggcc gcaacagttc gccaccgggg cctttccatc
     4801 gtcgccggcc ggctgcggcg cggtaccgtc atcgtggtgc tgatgagccg tgccgtctgc
     4861 gtgggcgtga accttgccgc cgtgatcgtg cgggccgacg tggccttcgg tcagacaatg
     4921 cgcgacggcg ggattgtcgg aaaatgcaaa ggccaccgac ggcgcgagaa tgcaaagcgc
     4981 gtagagcacg gcaagcacta tcgccgctct ggcgcgaaaa gtcttggtca aggccgacaa
     5041 catcgtgcgt cttgcgcact ccgatccgcg gaaaactcag gcgctccaag gatagacgag
     5101 tgaccgtggt ttgcaacgtt tcctggaagg ccggttcgca attcgggacg cgttttgcgg
     5161 cggaagcgtg gcgacgattg cccgtgtgat acaagggacg attgcccatg tgatacaagg
     5221 tatgtgacgg agcatccgac tactttaccg ccgccgctcc ccttgtcttc ggtacgatgg
     5281 ccagaaattc caaggactca tgcgcgagat tttctccggc atgtcgttga atcggcaccc
     5341 agtcgacgtc tcccgcgcgc cggtgaatgt cattggtgct gccgtcagcg tgagtgtctc
     5401 gcaagtatac gtcagtcaac cagaccacca atcgctcgct cgcaatgtcg tgcatcggcg
     5461 ttttctcgta tggcgccatc cggatgcgga cgacgacgat ggattcattt tcgaactcga
     5521 ccatggcatt agcagggccg gcctgcggga tcgcggcgcc gtgggacggc gttgagcgcg
     5581 cgccgggatg ttgcgcccag ccgggatcgg atccgaaggt caggaataga caagcggtat
     5641 agagccaaat tcttgtgagc ggatgtttca tgacgagtcg tcctggtgct cggtgactat
     5701 tgtttgacgt tcgcgtcgat cgaccttacg agttgaagcg cacttcaagt caaggagttt
     5761 ctttcatgcc tgggatgagc attggggagg tggccaagaa gatggggctg cggtcctcgg
     5821 ccattcgtta ctatgaaagg ctcggcctga tcccaaaagc gccgcggctg agcgggcgcc
     5881 gacgctatga cgatcgtgta cttgagcgac tggcgatggt ccgattcgca aaacacgtgg
     5941 gcttcagcat cgccgagatt aaggtgttgt tgcgcggggt cgacggccgc ccgccgcccg
     6001 agcggtggcg caagcttgcc gcgcacaagg ttgcgcaagt ggatgcattc gttgcgcagg
     6061 cccgaaacat ccgaaaaatg ctgtccgaaa cgctggactt ccagtgtccg agattggtgg
     6121 agcgcggtcg cgccctgcac tccacaaccg cacgtccagc cttacgcctg aagcgtaaca
     6181 ccagatcgta agtggatatt gctgattctg actgggtgca gatgtccgct atgagtcttg
     6241 ggtcgtctga ggctgctagc aatagattct gacgtcgggg ccggaagtca tcaaaggtac
     6301 gttcgtcggc atgcttcagc tatgcgcgat cacattgacc agccaaaagc ccgcaaggct
     6361 cagcaggctg acaaagacca gggcaacggc gtttatgacc atgcgatgcc ggtaatcatc
     6421 ctcgctttcc gggcactcgt atttcgagag gtcagggacc agtgagtaat ctggcccgga
     6481 atgaccgatt ggacccttcc cccgctgact tcggggcgaa gcatctgttc gtgatcggaa
     6541 tcgtattaca cggctgtcag cgtcggatga gagcaccgac gccggtaagc cccgccaagc
     6601 cccgacacat gggccgcaga tttttttacg cagctgctga tcttgtgggg tcacgatcgt
     6661 gtatcggagc agcgggcctg cctcaggatc gtccccctga aactcgacga tcgcgtgtgc
     6721 gggagctcgg cggctatggc cgtattcctc aaagtgcgag gcgcgaacga tctttaacga
     6781 cggatcccgc gcgaacgtcg ccaatgccgc gagttcggcg ctgctcgcac caccgacgcg
     6841 ccaggacggc tcgagcacgc ctgattgtgg ccaatcgtca gcgccgattc cctgagcgac
     6901 atcgtagttc cggcgcgatg ctatgaaacc agaaaattcc tccgccgccg cgtcgtactg
     6961 tcttgctgca gctaccgcgg cgcgtacctc ggacggcatg ggaagtgcat cgagactctt
     7021 ccacccgccg cgcacgcata cgaggtccga cccgccgtaa actggccgcc cctcgttgtc
     7081 cctgacagtc ctttgcgttc cgtgatagct gatcctaagg ctcccgattg taacctcccc
     7141 gatgctcaac gtcctgactt ggcgcaagtt ctcttcgagg accaacccat atgtggcgat
     7201 ttcgtcggca gtgatatttt ccaggtctgc atcgagttca ttcagactcg taaccagcgt
     7261 ctgatccctt cccgacgcac tgagcggttt cttcaggcgg aggggtccac gcctgagcat
     7321 gcgtctggcc gccacccgcg catcgcggtt gctgaacacg gtgtagcccg gcaacacgat
     7381 ctcgcgcact cgctcggcga aagccgtgca ccagccctgc ggtcgctccg cgtcacgatc
     7441 gaccaggcca tgggtgatgg ctttcgtctt cacgaaaaga tggggcacaa cgccgccgta
     7501 gagatcattc gcagaacgaa tgccgagacg tgatgcctcg tccagcagca aggtgtcgcc
     7561 aggcacgaaa aagagagggt cggaatagtc acatcccgac tcatagtgcc ccgaaaagtc
     7621 atagcccttg atcttggcga tagatttggc gcccaagtcg agcacgacct tgtgatgcgt
     7681 ctgcagcggc ttgccgaacc gcgagaagta gaccacgacg acgcctgttg ccatggtaat
     7741 cgacccttca tccatgcgcc cagtccacga tggcggctga gtggcactcc cgagagtgcg
     7801 ttcttccttc agcgcgccga tcctctcgtc tgggcagacg agcacgatcg ccctgatgct
     7861 gcagaggggt gtttaaggca ggtttatgcc gagttggacg ggatgatcgg cggcagtcac
     7921 aagtccgaga ggtgtgtgtt gtcaaccagg tctgccggta tggtcgacgc aagaacggtg
     7981 gacatgttgg aactgagatt gcgcctcaac ttacggtatt gggcgaaata cccagagggc
     8041 tgctggttcg ccgggattgg cacgagcggc gacgaaaaac cggtcgagtt cgggaacaaa
     8101 cagcgcagtt cgtgcgccgg atatcgtcgg gatgtgggcc gagcggccgt aggcgtcccc
     8161 ttgcgcctcg aacacatcca cgtatccgtc gccgcagctg aggtacacgc gctggcgctt
     8221 cgcatcgaag aagag
//
LOCUS       DATLJT010000972         5764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4430549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000972 DATLJT010000000
VERSION     DATLJT010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4430549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /gene="aspS"
                     /locus_tag="VM910_22420"
     CDS             <1..1461
                     /gene="aspS"
                     /locus_tag="VM910_22420"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HVI65312.1"
                     /translation="GPAGELPLPVFGEQEYPEDIRLKYRFLDLRREKLHQNIMRRGAI
                     IDSMRRRMKEQGFFEFQTPILTASSPEGARDFLVPSRIHPGRFYALPQAPQQYKQLLM
                     MSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDIEMSFVTQEDVFAAMEPVIGGVFEEF
                     AQGKPVTKNWPRIPFAEAIRKYGSDKPDLRNPIVMQEVSEHFRGSGFKVFARLLEDAK
                     NQVWAIPATGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGNEGAGPLANNIGPERTAA
                     ISTQLGLKGGDAAFFVAGDPDKFWKFAGLARVRLGEELNLVDKDRYELAWVVDFPMYE
                     YDEENKKIDFSHNPFSMPQGGLEALTTQDPLTIKAFQYDITCNGYEIASGGIRNHRPD
                     AMVKAFEIAGYGEKEVVERFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTTNLREIS
                     LFPMNQQAVDLLMGAPSEVTPKQLRELHIRLNLPQN"
     gene            1519..3825
                     /locus_tag="VM910_22425"
     CDS             1519..3825
                     /locus_tag="VM910_22425"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HVI65313.1"
                     /translation="MSYSEDLHSAALAYHRLPRPGKLEIQAIKPLANQRDLALAYSPG
                     VAAACTEIAGNPAEAASLTARANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKK
                     FAGIDVFDIEIAADTIDRVVETVAALEPTFGGINLEDIKGPDCFEIEARLRERMKIPV
                     FHDDQHGTAIIVGAAIVNALLLNGKKLPDVKIVCSGAGAAALACLNLLVSMGAQRKNI
                     WVCDIDGVVYEGRPTLMDRWKAVYAQKTDKRKLADVIVGADIFVGLSAPNVLTQDMVK
                     QMADKPLVMALANPNPEIMPDEARAARPDAMICTGRSDFPNQVNNVLCFPFIFRGALD
                     VGATAINEEMKHAAVDAIAQLARDPPSDVVARGFEAGEVQGFGPGSLIPSPFDPRLIQ
                     RIAPAVAKAAMESGVATRPIKNFDEYTALLERFAFRSGLVMKPVFAKAKTQPVRVIYA
                     EGEDERVLRATQQVLEESLAKPILVGRPSVVEVRIKRFGLSIKAGKDFDLVNPEDDPR
                     YRSYVQSYIEVAGRRGITPDAARTVVRTNNTVIAALAVHRGEADAMICGVEGRYMSHL
                     RHVREIIGHRPEVSDFAALALMITTKGAYFIADTEVRSNPSADELCEMAALAANHVQR
                     FNLKPKIAFLSHSDFGSYDTDSSRKMRRATALFKEHHPELEADGEMQGDTALSEATRK
                     LILPHSNLEGEANILMMPNLDAANVAYQMIKVLADAVPVGPILIGPSRPAHILTPSVT
                     ARGILNMTAVAAVEAQERAGRQQPNLFS"
     gene            3966..4418
                     /locus_tag="VM910_22430"
     CDS             3966..4418
                     /locus_tag="VM910_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI65314.1"
                     /translation="MPAFIIFGRVLFAVLFIYSGATKLFAIQPTADAIAAKVTFPAIL
                     APYTQQLETLTGMPTPQVLAIAGGAFEILAGLMIALNFGVRFFAFLLIIFVAATTYYF
                     NDFWNQVPPDNGKTLIDALKNLSIIGALFIIAGYGRGHRMAEPAYGDL"
     gene            complement(4431..4940)
                     /locus_tag="VM910_22435"
     CDS             complement(4431..4940)
                     /locus_tag="VM910_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HVI65315.1"
                     /translation="MLANADGVMPDALKFEGDKRFFSTALDRVALIVHGRNSHEHQPN
                     SARRRRVILTRSISALASDPSNLMATLWNPHGASFEQACGFAGISSGTVAIIGGPVVF
                     GMFLDRYDTFWLSQAPHVRLPGGKGCFTGVPPRAPQDILAAHGMKAGEPQVLEASEDV
                     SVTPWRRAA"
     gene            complement(5012..>5764)
                     /locus_tag="VM910_22440"
     CDS             complement(5012..>5764)
                     /locus_tag="VM910_22440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI65316.1"
                     /translation="TVEIDTTGGPGFGFEHFKELDFLRDKEVVLTFDDGPWPVNTPSV
                     LKTLESECTTGIFFPIGKHATYEPELLKKVYAAGHTIGSHTWSHANLNNKKLSEDQRK
                     EEIEKGLSAIKWALGGVSPAPFFRFPALQHPPEMVTYLGTRNMAIFSCDLDSFDFKAS
                     KPQTVIDNVMKKLDKLGKGIILMHDFQKHTAEALPDLLKRLKDGGYKVVAMRAKAPAQ
                     TLPEYDQQLLKEIKLPTVSSRPVSSVVTDVSQ"
BASE COUNT         1103 a         1763 c         1791 g         1107 t
ORIGIN      
        1 gggcccgccg gcgaattgcc gctgcctgtg ttcggcgaac aggaataccc ggaagatatc
       61 aggctgaaat accgcttcct cgacctgcgc cgcgaaaagc tgcatcagaa catcatgagg
      121 cgtggcgcga tcatcgattc catgcgtcgg cgcatgaagg agcagggctt tttcgagttt
      181 cagaccccga tcctgaccgc ttcttcgccg gaaggcgcac gcgatttcct ggtgccgtca
      241 cgcattcatc cgggcaggtt ttacgcgctg ccgcaggcgc cgcagcaata caaacagctt
      301 ttgatgatgt cgggcttcga ccgctatttc cagatcgcgc cgtgcttccg cgacgaggac
      361 ccgcgggccg atcgtctccc cggcgagttt tatcaactcg acatcgaaat gagctttgtc
      421 acgcaggaag acgtattcgc agcgatggag cctgtcatcg gcggcgtgtt cgaggaattc
      481 gcgcaaggca agccggtgac gaaaaactgg ccccgaattc ccttcgccga agcgatccgc
      541 aaatatggca gcgacaagcc cgacctgcgc aatccgatcg tcatgcaaga ggtttccgag
      601 cattttcgtg gctccggctt caaggtgttc gcgcggctgc ttgaagacgc aaagaaccag
      661 gtttgggcga tcccggcgac cggtggcggc agtcgcgcgt tctgcgaccg catgaactcg
      721 tgggcgcaag gggaggggca gccgggcttg ggctacatca tgtggcgcga aggcaatgag
      781 ggcgcgggcc ccttggccaa caacatcggt ccggagcgaa cggcggcgat cagcacccag
      841 ctcggcctga agggaggcga cgcggcgttt ttcgtggccg gcgatccaga taaattctgg
      901 aagtttgcag gactcgcgcg cgtcaggctc ggagaggaat tgaacctcgt cgacaaggac
      961 cggtacgagc tcgcttgggt cgtcgacttc ccgatgtatg agtacgacga agaaaacaag
     1021 aagatcgact tctcgcacaa cccgttctcg atgccgcagg gtgggctcga agcgcttacg
     1081 acgcaggacc cgctgacgat caaggcattc cagtacgaca tcacctgcaa cggctacgag
     1141 attgcatccg gcggtatccg caaccaccgt ccggatgcga tggtgaaggc gttcgagatc
     1201 gcgggctatg gcgagaagga ggtcgtggag cgtttcggcg gcatgtaccg cgctttccag
     1261 tatggcgcgc cgccacatgg cggcatggcg gcaggcgtcg accgcatcgt catgcttctg
     1321 tgcggcacca ccaatctgcg cgagatttcg cttttcccga tgaaccagca ggcggtggac
     1381 ctgctgatgg gggcaccgtc ggaggtcact ccgaagcagt tgcgcgagct tcacatccgg
     1441 ctcaatctgc cccaaaactg acccactgaa ccacccgaga tcgatgtttc ggggtcagtg
     1501 aagcctccgg gggagcgaat gtcctattcc gaagacctgc attcggcagc actggcctat
     1561 caccgcctgc ctcggcccgg aaagctcgag attcaggcca tcaagccgct cgcgaaccaa
     1621 cgggatctcg cgctcgcata ttctcccggc gttgccgcag cctgcaccga gattgccggc
     1681 aatccggccg aagccgcttc gctgacggcg cgcgccaacc ttgttgcggt cgtctccaac
     1741 ggtaccgcgg tgctgggcct cggcaatatc ggtccactcg cctcaaaacc cgtgatggag
     1801 ggcaaggcgg tcctcttcaa gaaattcgcc ggcatcgacg tgttcgacat cgagattgcc
     1861 gccgacacca tcgatcgcgt ggtggagacg gtggccgcgc tggagccgac cttcggcggc
     1921 atcaatctcg aagatatcaa gggtccggac tgcttcgaga tagaggcgcg cttgagggag
     1981 cgcatgaaaa ttccggtctt tcacgacgat cagcatggca ccgcgatcat cgttggagct
     2041 gcgatcgtca acgcattgtt gttgaacggc aagaaacttc cggacgtgaa gatcgtgtgc
     2101 tccggtgccg gtgctgcggc gctggcttgc ctcaatcttc tggtctcgat gggcgcgcag
     2161 cgaaaaaata tctgggtctg cgacatcgat ggcgtggtgt acgaggggcg tcccacgctg
     2221 atggaccgat ggaaggcggt ctatgcgcag aagaccgaca agcgaaagct cgctgacgtc
     2281 atcgtcggcg ccgacatttt tgtggggctg tctgcgccaa acgtgctgac gcaggacatg
     2341 gtcaagcaga tggcggacaa accgctcgtc atggcgcttg ccaatccgaa cccggagatc
     2401 atgccggacg aggcgcgcgc ggcgcggccc gatgcgatga tctgcaccgg gcgatccgat
     2461 ttcccaaacc aggtcaacaa cgttctctgt ttcccgttca tcttccgcgg cgctctcgac
     2521 gttggcgcca ccgccatcaa cgaggagatg aagcatgccg cggtcgatgc cattgcgcag
     2581 ctcgcacgcg atccgccgtc ggatgtggtc gcgcgcggct ttgaggcggg cgaggtgcaa
     2641 ggcttcggac cgggctcgct gatccccagc ccgttcgatc cgcgactgat ccagcgcatc
     2701 gcgccggcgg tggcgaaggc ggcgatggaa tccggggtcg caaccaggcc catcaagaac
     2761 tttgacgagt acaccgctct gctcgagcgt ttcgcgttcc gctccggtct cgtcatgaag
     2821 ccggtctttg ccaaggccaa gacccagccg gtccgcgtca tctatgcgga aggcgaggac
     2881 gagcgcgtgc tgcgcgcgac ccagcaggtg ctcgaggaaa gcctggcaaa gccgatcctg
     2941 gtgggacgtc cgtcggtcgt cgaggttcgc atcaagcgtt tcgggctctc gatcaaggca
     3001 ggcaaggatt tcgacctggt caatcccgag gacgacccgc gttatcgctc ctacgtgcag
     3061 tcctatatcg aggtcgcggg ccgccgcggc atcacccccg atgccgcgcg caccgtggtg
     3121 cgcaccaaca acacggtgat cgccgcgctt gcggtgcacc gcggcgaggc ggacgccatg
     3181 atctgcggcg tagaaggccg ctatatgagc catctgcgcc acgtgcgcga gatcatcggc
     3241 catcggcccg aggtcagcga tttcgcggcg ctcgcgttga tgatcacaac caagggcgcc
     3301 tatttcattg cagataccga ggtgcgttcg aatccgagcg cggatgaact ctgcgaaatg
     3361 gcggcactcg ccgccaacca cgtccagcgc ttcaacctca agccgaaaat agcgtttctg
     3421 tcacattcgg atttcggcag ctacgatacc gactcctcac gcaagatgcg gcgcgccacc
     3481 gcgcttttca aggagcacca tccggagctc gaagccgacg gcgaaatgca gggcgatacc
     3541 gcgctctcgg aagcgacgcg caagctgatc ctgccgcatt cgaatctcga gggcgaggcc
     3601 aacatcctga tgatgccgaa tctggatgcc gccaacgtcg cctatcaaat gatcaaggtc
     3661 ttggcagacg cggtcccggt tgggccgatt ttgatcggcc cgtcgcgacc ggcccatatc
     3721 ctcaccccct cggtcaccgc gcgcggcatc ctgaatatga ctgccgttgc cgccgtcgaa
     3781 gcccaggagc gtgccggccg acagcagccg aacctgttca gttagatgaa gggcggcgtt
     3841 ttccaggtac gacgcgcatc cgcgcgccgc accatttgag cgtttgaaaa gcgcagacgg
     3901 aacctccata atccgcctgt tttgccgttc aggatctgcg cccctaagct tcggtgaggc
     3961 cgaccatgcc agcgttcatc attttcgggc gagttctgtt cgccgtcctt ttcatctatt
     4021 cgggggcgac aaaactattc gccattcagc ccacggcaga tgccatcgcc gccaaggtca
     4081 cgtttcctgc gatccttgcg ccctacaccc agcaattgga gacgctgacg ggaatgccga
     4141 cgccgcaggt cctggcgatc gcgggcggcg ctttcgaaat tcttgccggc ttgatgatcg
     4201 cgctgaattt cggtgtgcgg ttcttcgcct ttctcctgat catcttcgtc gctgcgacga
     4261 cctactactt caacgatttc tggaaccagg tgccgcccga caatggcaag acgctgatcg
     4321 acgccttgaa gaatctttcg atcatcggcg cgctgttcat aatcgcggga tatggcaggg
     4381 gccacaggat ggccgaaccc gcctatggcg atctctagtg gccagaccgt tcaggcagcg
     4441 cgccgccacg gcgtaacact gacatcttcc gacgcctcca gcacctgcgg ctcgccggct
     4501 ttcatgccgt gcgcggcgag gatgtcctgc ggcgcccgtg ggggcacgcc cgtgaagcaa
     4561 cctttaccgc cgggcagccg gacgtgcggc gcttgtgaca gccagaaggt gtcgtagcgg
     4621 tcgagaaaca tgccgaacac gacagggccg ccgatgatgg cgacggtgcc ggaggagatg
     4681 ccggcgaagc cgcaggcctg ttcgaacgat gccccgtgcg gattccacaa ggttgccatg
     4741 agattggacg gatcggacgc cagcgccgaa atgctgcggg tcaggatgac ccgcctgcgg
     4801 cgcgccgagt tcggctggtg ctcgtgcgag ttgcggccgt gcacaatcag tgcgacacgg
     4861 tcgagggcgg tcgagaaaaa ccgcttgtcg ccctcgaact tcaacgcatc cggcatcacg
     4921 ccatcggcat tcgccaacat gccgtcggcg gagacgatga cgtagccctc gatgcgcagc
     4981 ggctgcacgg ccgtgccagg atctcccgtg gttattgcga gacgtcggtc acaacgctgg
     5041 agacggggcg ggagctcacg gtcggcagct tgatctcctt gagcagttgc tgatcgtatt
     5101 ccggcagcgt ttgcgccggc gcctttgccc gcatcgccac gaccttgtat ccgccgtcct
     5161 tcagccgctt gaggagatcg ggcaacgctt cggcggtgtg cttctggaag tcatgcatca
     5221 ggatgatgcc cttgcccagc ttgtcgagct tcttcatcac gttgtcgatg accgtctgcg
     5281 gcttgcttgc cttgaagtcg aaggaatcga gatcgcagga gaaaatcgcc atgttgcggg
     5341 tgccgagata ggtgaccatt tccggcggat gctgcagcgc cggaaaacgg aagaaaggtg
     5401 cgggcgagac gccgccgagc gcccatttga tcgcgctgag gcctttctcg atctcttcct
     5461 tgcgttggtc ctcgctgagc ttcttgttgt tgagattggc gtgagaccag gtatgcgatc
     5521 cgatggtgtg gccagccgca tagaccttct tcaacagctc cggctcatag gtcgcatgct
     5581 tgccgatcgg aaagaagata ccggtcgtgc attcgctctc aagcgtcttc agaaccgatg
     5641 gtgtattgac cggccacggg ccgtcgtcga aggtgaggac gacttcctta tcgcgaagga
     5701 agtcgagctc cttgaaatgt tcaaagccaa aaccggggcc gccggtggta tcgatctcga
     5761 cggt
//
LOCUS       DATLJT010000973         1604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2822454,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000973 DATLJT010000000
VERSION     DATLJT010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2822454"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VM910_22445"
     CDS             complement(<1..1151)
                     /locus_tag="VM910_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVI65317.1"
                     /translation="MNLFRLLEARHKAGKPVRVALIGAGKFGSMFLAQVPHVAGIEVP
                     VIVDLDPQRAKDACRTVGWDDTRIAATTFTPDAAKATAGEIEVVVDATGHPAAGIRHA
                     RAAIASGKHIVMVNVEADVLAGPLLAEEARRAGVVYSLAYGDQPALTAEIVDWARATG
                     FRVVAAGKGTKYLPEYHDVTPDNVWQHYGLTRSEAQSAGMNPQMFNSFLDGTKSAIEM
                     AAIANATALDVPSGGLLFPPCGVDDLPHVMRPRDKGGVLEKAGVVEVVSSLERDGRPV
                     FRDLRWGVYAVLEAPNDYAADCFRQYGLKTDASGRYAAMYKPYHLIGLELNISILSAV
                     LRREPTGQAQGFCGDVAAVAKRNLRAGEVLDGEGGYTVWGKLMPAAASLA"
     gene            complement(1287..>1604)
                     /locus_tag="VM910_22450"
     CDS             complement(1287..>1604)
                     /locus_tag="VM910_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVI65318.1"
                     /translation="ETTSYPLWTLDEETVTRLVKQGGIVAPTGKAGGMLMFHGNLVHG
                     SSGNITPYPRKIVYLTLNAVSNYIRTPTRPDYIAHRDFTPIKAVDDDALLRLARAPRQ
                     AAE"
BASE COUNT          282 a          520 c          509 g          293 t
ORIGIN      
        1 gccaggcttg cagcggccgg catcagtttg ccccagaccg tatagccgcc ttcgccgtcg
       61 agcacctctc cggcgcgcag attccgtttg gcaacggcag cgacatcgcc gcagaagccc
      121 tgcgcctgcc cggtcggctc cctgcgcagc accgccgaca ggatcgaaat gttcaattcc
      181 agcccgatca gatggtaggg tttgtacatc gcggcgtaac gcccgctcgc atcggtcttg
      241 agtccatatt gccggaaaca gtcggcggca tagtcgttgg gcgcttccag cacggcatac
      301 acgccccagc gcaggtcgcg aaacaccggt cgtccgtcgc gctcgagcga cgacaccacc
      361 tccaccacgc ctgctttctc gagcacgccg cctttgtcac gcgggcgcat cacatgtgga
      421 agatcgtcga cgccgcacgg cggaaacaac aggccgcccg atggcacgtc gagcgcggtc
      481 gcgttcgcaa tcgccgccat ctcgattgcg gacttggtgc cgtcgaggaa ggagttgaac
      541 atctgcgggt tcatgccggc ggattgcgct tcgctgcggg tgagcccgta atgctgccag
      601 acattgtcgg gcgtcacgtc atgatattcc ggcagatatt tggtgccctt gccggcggcg
      661 acgacgcgaa agccggtggc ccgcgcccag tccacgattt cggccgtcag cgccggctga
      721 tcgccatagg cgagcgaata aaccaccccc gcgcgacgcg cttcttccgc gagcagcggt
      781 ccggccagca cgtcggcctc gacattgacc atcacgatgt gcttgccgga agcgatcgcg
      841 gcgcgtgcgt ggcggatgcc ggcagcagga tggccggttg catccacaac cacctcgatc
      901 tcgcctgccg tcgccttcgc cgcatcaggg gtgaatgtcg tggccgcaat ccgcgtgtca
      961 tcccagccta cggtcctgca ggcgtccttt gcgcgctgcg gatcgagatc gacgatcacg
     1021 ggcacctcga tgcctgcaac gtgaggcact tgcgccaaaa acatcgaacc gaatttgcct
     1081 gcgccgatca gcgcgacacg aacgggtttg ccggccttgt ggcgggcttc aagaaggcgg
     1141 aagagattca tggggataca tccgatcaat gaaagcgtca tccggggcga cgcataagga
     1201 tcgcgcccgg aatccatctt cgccatggtg gttatggatt ccggactagc accttttgtc
     1261 gcgcctcgaa atgacggtgg tgaaaactac tccgcggcct gccgcggcgc gcgggcgagg
     1321 cgcaagagcg cgtcgtcgtc caccgccttg atcggcgtga agtcgcgatg ggcgatatag
     1381 tccggccgcg tcggtgtgcg gatgtagttg gaaaccgcgt tcagcgtcag gtagacgatc
     1441 ttgcgcgggt agggcgtgat gttgccggac gagccgtgca cgagattgcc gtggaacatc
     1501 agcatgccgc cggccttgcc ggtcggcgcg acgatgccgc cctgcttgac cagccgcgtc
     1561 actgtctctt cgtccagcgt ccacagcgga taggacgtgg tttc
//
LOCUS       DATLJT010000974         1583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3051962,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000974 DATLJT010000000
VERSION     DATLJT010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051962"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VM910_22455"
     CDS             <1..855
                     /locus_tag="VM910_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HVI65319.1"
                     /translation="NDLLRLLGGREIHPINARIGGFYRVPNRAELAPLAEALKHARDR
                     AIESLRWTAGFPFPEFVRDYEFVALGHPDEYPMSAGRIVSSAGLDIAAADYETEFIER
                     QVPYSTALHSVLKGRAAYLVGPMARYALNRDRLPADIRTLAEEVGLEPVCRNPFRSIL
                     VRGVEVVYACHEALRLIAAYEPPSRPFVPAEPRAGVGYACTEAPRGILYHRYRIDADG
                     TILDASLVPPTAQNQDVIEEDLREVATAGLALDEEALKARCEQSIRNHDPCISCAAHF
                     LKLRVDRT"
     gene            852..1352
                     /locus_tag="VM910_22460"
     CDS             852..1352
                     /locus_tag="VM910_22460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HVI65320.1"
                     /translation="MTGDEQPLALVVGIGNRDRGDDGFGPAVAHRLMERVPSTVRILE
                     RSGDALAMIEDWDGIPSVIVIDAMAPIRKPGKVHRFDLTGSPLPIGFAPASSHAFGVA
                     ETVELARSLGRLPQCLVAYLVEGEQFGTGAPLSLAVAEAVEHVAGRIVSEVLTILKAK
                     GKGRHA"
     gene            1345..>1583
                     /locus_tag="VM910_22465"
     CDS             1345..>1583
                     /locus_tag="VM910_22465"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HVI65321.1"
                     /translation="MHETALVRDVVRRIEDLVQATGARRITGAKIWLGALSHLSAEHF
                     REHYAIEAQGSIAAGAILTIEASSDLDHPDAQHVRL"
BASE COUNT          287 a          484 c          490 g          322 t
ORIGIN      
        1 aacgacctct tgcgcttgct tggcgggcgc gagatccatc caataaatgc acgcattggc
       61 ggattctacc gagtgcccaa ccgcgccgag ctggcgccgc ttgccgaagc gctgaagcat
      121 gcgcgcgatc gcgcgatcga atcattgcgc tggacggcgg gctttccatt tccggaattt
      181 gtccgcgact acgagttcgt ggcactcggc catcctgacg aatacccaat gagcgctggg
      241 aggatcgtct cctctgcggg tctcgacatc gccgctgcag actatgaaac cgaattcatc
      301 gaacgtcagg tgccatattc gacggcgctt cattccgtat tgaaggggcg tgccgcctat
      361 ttggttggac cgatggcgcg ctatgcgctc aaccgggacc gcttgccggc cgatatccgc
      421 acgcttgccg aagaggtcgg gctcgagccg gtgtgccgca atccattccg cagcatcctc
      481 gttcgcgggg tagaagttgt ttacgcctgc cacgaggcat tgcgtctgat tgccgcctac
      541 gagcccccat cgcggccttt cgtcccggcc gagccgcgtg ccggcgtcgg ctacgcctgc
      601 accgaggcgc ctcgcggcat cctttatcat cgctaccgaa tagacgccga cgggacgatt
      661 ctcgatgcct ccctcgttcc gccgaccgcg caaaatcaag atgtcatcga ggaagatctg
      721 cgagaggtcg caacggccgg gcttgctctc gacgaggagg cgctgaaggc ccggtgcgag
      781 cagagcattc gaaaccatga cccctgcatt tcctgtgctg cgcatttctt gaaactgcgg
      841 gtggatcgga catgacgggc gacgagcagc ccctggcgct cgtcgtcggc atcggtaacc
      901 gggatcgtgg tgacgacgga tttggtccgg cggtcgccca tcggcttatg gaacgtgtgc
      961 cctccacggt ccgcattctc gaacgcagcg gcgatgctct cgccatgatt gaggattggg
     1021 acgggatccc ctctgtcatt gtcatcgatg caatggcgcc gatcagaaaa ccgggcaaag
     1081 tgcatcgctt cgatctcacg ggcagcccgc tgccgatcgg tttcgcgccg gcctccagtc
     1141 acgctttcgg agtcgcggag accgttgagc tggcgcgcag tctaggtagg ctgccccagt
     1201 gtctcgtcgc ctaccttgtc gaaggcgaac agttcgggac gggggcgcca ttgtcgttag
     1261 cagttgccga agctgttgag catgtggctg gccgcatcgt cagcgaagtt ttgacgatac
     1321 tgaaggcaaa agggaaaggt cgacatgcat gaaactgcac tggtgcgtga cgttgtccgc
     1381 cgtattgaag acctcgtcca ggcgaccggc gcacgtcgta tcacgggtgc taagatctgg
     1441 cttggcgcgt tgagccacct ttcggccgag cattttcgtg agcattacgc catcgaggcg
     1501 caaggatcga tcgccgccgg agcgatactc acgatcgagg cgtcgagcga tctagatcac
     1561 ccagacgccc agcatgtccg gct
//
LOCUS       DATLJT010000975         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2361982,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000975 DATLJT010000000
VERSION     DATLJT010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361982"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VM910_22470"
     CDS             complement(<1..252)
                     /locus_tag="VM910_22470"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145174.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVI65322.1"
                     /translation="MNQHFDTILKSGAVVNQDGEGVRDIGIRGGRIAEIGGLGQASAA
                     EVIDCKGLHILPGVMDTQVHFREPGLTHKEDLETGSRSAV"
     gene            372..1253
                     /locus_tag="VM910_22475"
     CDS             372..1253
                     /locus_tag="VM910_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HVI65323.1"
                     /translation="MKAAFLPDRGVVKVTGEDARNFLNGLVTTDVTELGPARGRFGAL
                     LTPQGKIVVDFLITEAPGGHGGGFLFDCPRALAKTLTDKLTFYKLRAKIAVENLSDSL
                     GVIAAWEGDPAVTPDLAFADPRDQALGWRILAPQELAAKVAALIGADLVDASAYEAHR
                     IACGAPRGGLDFTYGAAFPHETNMDRLHGVDFDKGCYVGQEVVSRMQHRGSARTRTVK
                     VLLDGPSPEPGATILAGDKAVGTMGSTAGEMGLALIRTDRVADALSAGLALTAGGLGI
                     RLAEPQALQPVQSQTIA"
     gene            1250..1876
                     /locus_tag="VM910_22480"
     CDS             1250..1876
                     /locus_tag="VM910_22480"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVI65324.1"
                     /translation="MSRSARLHADGKIRCPWPGEDPLYVTYHDTEWGVPEYDDRALYE
                     KLILDGFQAGLSWITILRKRDNFRRAFDDFRPEKIARYNAKKIHALMNDAGIVRNRAK
                     IEGAIESAKSYLKIMEQGPGFSKLLWDFVDGRPKVNNFKTTASVPAATPLSIKISKEL
                     SSRGFKFVGPTIVYAFMQATGMVNDHLVTCFCHESCGKAHRAPRLKIK"
     gene            1873..2484
                     /locus_tag="VM910_22485"
     CDS             1873..2484
                     /locus_tag="VM910_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HVI65325.1"
                     /translation="MTAEKPSAAGARVWQRMLSGRRLDLLDPSPLDIEIADIAHGLAR
                     VARWNGQTSGAHIFSVAQHTLLVEAVMRAQNPRLDARVRLAALLHDAPEYVIGDMISP
                     FKAVLGGDYRVVEKRLLAAIHVRFGLPPQLPAEVEQRIKDADRGAAYLEATELAGFAE
                     TEAKRLFGRDPTLPDSIRQDYLTPWSAAKAEKRFLARFKALAA"
     gene            2557..>2673
                     /locus_tag="VM910_22490"
     CDS             2557..>2673
                     /locus_tag="VM910_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HVI65326.1"
                     /translation="MIHVCSLAALPETVKRTGASHVLTVMANVDQVQRPASVL"
BASE COUNT          504 a          883 c          807 g          479 t
ORIGIN      
        1 gaccgcgctg cgcgagccgg tctccaggtc ttccttgtga gtcaatccgg gctcgcggaa
       61 atgcacctgc gtgtccatca cgccgggcag gatgtgcaac cccttgcagt cgatcacttc
      121 ggctgcagaa gcctgcccca gaccgccgat ttcggcgatt cggccgcctc tgatgccgat
      181 gtcgcggacg ccctcgccgt cctgattgac caccgcgccg gattttagaa tggtgtcaaa
      241 atgttgattc atcgcttctc agcgcccaca tcagggaagc caggccttgt gagcggcacc
      301 ttagcggctt agcctgccac gtgatattgc ttcaacaaaa acatcaagag aaacttggca
      361 agagacttca aatgaaggca gcgtttcttc cggaccgtgg cgtggtcaag gtgaccggcg
      421 aggacgcgcg caacttcctc aacggcctcg tcacaaccga cgtgacggag cttgggcctg
      481 cgcgggggcg attcggcgcg ctgctgacgc cgcagggcaa gatcgtcgtg gacttcctga
      541 tcacggaggc gcccggcggc catggcggcg gcttcctctt cgactgcccg cgcgcgcttg
      601 ccaagacgct gaccgacaag ctcactttct acaagctgcg cgccaagatc gctgttgaaa
      661 acctgtccga cagccttggc gtgatcgccg cctgggaagg cgatccggcc gtaacgcccg
      721 atcttgcttt tgccgatccg cgggaccagg cacttggctg gcgcattctt gcgccgcaag
      781 aacttgccgc gaaggtcgca gctctgatcg gtgccgacct cgtcgatgcc agcgcctacg
      841 aagcccaccg catcgcctgc ggcgcgccgc gcggcggtct ggacttcacg tatggcgctg
      901 cattcccgca tgagacaaac atggatcggc tgcacggcgt cgatttcgac aagggctgct
      961 atgtcggcca ggaagtggtg tcgcgaatgc aacatcgcgg ctccgcacgc acccgcacgg
     1021 tgaaggtcct gctcgacgga ccctcgcccg aacccggcgc caccattctc gccggcgaca
     1081 aggcggtcgg caccatgggc tcgaccgcgg gcgaaatggg gctcgcactg atccgcaccg
     1141 accgcgttgc ggatgccctt tccgccgggc tcgcattgac cgcgggaggg ttaggcatcc
     1201 ggctcgccga accgcaggcg ctgcaacccg tccagtcgca gaccatcgca tgagccgctc
     1261 cgcacgactg catgccgacg gcaagattcg ctgcccctgg ccgggcgagg atccgcttta
     1321 tgtcacctat cacgacaccg aatggggcgt gccggaatat gacgaccgtg cgctctacga
     1381 gaagctgatc ctcgacggtt tccaggccgg actgtcgtgg atcaccatcc tgcgcaagcg
     1441 cgacaatttc cgccgcgcct tcgacgattt tcggcccgag aagatcgcgc gctacaatgc
     1501 gaagaaaatt cacgcgctga tgaacgacgc cggcatagtt cgcaaccgcg ccaagatcga
     1561 gggcgccatc gagagcgcga agtcctatct gaagatcatg gagcaaggcc cgggcttctc
     1621 gaagcttttg tgggatttcg tcgacggcag gccgaaggtc aacaacttca agaccacggc
     1681 gagtgtgccg gcagcaacgc cgctctcgat caagatctcg aaagagctct cttcccgcgg
     1741 tttcaaattc gtcggtccca ccatcgtcta tgcgttcatg caggcaaccg gcatggtcaa
     1801 cgaccatctc gtgacctgct tctgccacga gagctgcggc aaggcgcacc gcgcgccccg
     1861 tctcaaaatc aaatgacggc agaaaaaccg tccgcagcgg gcgcacgcgt ctggcagcgg
     1921 atgctgtcgg ggcggcggct cgatctgctc gatccctcgc cactcgacat cgagatcgcc
     1981 gacatcgccc atggcctggc acgtgtagcg cgctggaacg gccagaccag cggtgcacac
     2041 atcttctccg tcgcccagca cacgcttctg gtcgaagcgg tcatgcgcgc gcagaacccg
     2101 aggctcgatg cacgcgtgcg gcttgcggcg ctgcttcacg atgcgccgga atatgtcatc
     2161 ggcgacatga tctccccgtt caaggcggtg ctggggggcg actacagggt ggtggaaaag
     2221 cggctgcttg ccgcaatcca cgttcgcttc ggcctgccgc cgcagcttcc cgccgaggtc
     2281 gagcagcgca tcaaggatgc cgatcgcggt gcggcctatc tggaggcgac cgagcttgca
     2341 ggctttgccg aaactgaggc caaacgcctg ttcggccgcg atcccaccct gcccgattcg
     2401 atccggcagg actatctgac gccctggagc gcagccaagg cggagaagcg gttcctggcc
     2461 cgattcaagg cattagcggc ttgaaagcag accacgaacc tgctttcacg gcctcgcttg
     2521 cgcgcctata ttctgcgcga caaaagaagg tccgccatga ttcacgtctg ctcgcttgcc
     2581 gccctgccgg aaaccgtgaa acgaaccggc gcaagtcatg tgctcaccgt tatggccaat
     2641 gtcgaccagg tccagcggcc ggcatcggta ctt
//
LOCUS       DATLJT010000976         5615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2593474,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000976 DATLJT010000000
VERSION     DATLJT010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593474"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(882..1103)
                     /locus_tag="VM910_22495"
     CDS             complement(882..1103)
                     /locus_tag="VM910_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65327.1"
                     /translation="MPIRSSLERDHSFGPEDIARLSAAFETALGKLGLVDRKDPATTA
                     VAKLIIELAKEGERDPERLCALALKQLSK"
     gene            complement(2508..3401)
                     /locus_tag="VM910_22500"
     CDS             complement(2508..3401)
                     /locus_tag="VM910_22500"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI65328.1"
                     /translation="MKQLLLGCVPLAALAAISSATAADLPVRKAPPVVPAFSWTGCYL
                     GGYVGGAWQGGDGATFTDKGQNGIGPAGSIAVPPFLSYSGGAVAARLVPAHSWNADLG
                     SSVIGGGTLGCNWQPVNSPFVLGLEAEGGYMHLSGDAFDPRTIISTQTVPDVLGSAKV
                     GDWYGMVTGRLGYAWDRWLLYVKGGAAFVRTEASVLDACQNTVIGCGNWLISTSGSKT
                     VTTWTVGGGLEWAFAQSWSVKGEYMFIGLNDRSGFQTCGPVTTPSGAILAGGPFCFDH
                     RFDGIHTAKIGLNYRFGQIVY"
     gene            3665..3949
                     /locus_tag="VM910_22505"
     CDS             3665..3949
                     /locus_tag="VM910_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HVI65329.1"
                     /translation="MPMSPVIEITYCRLCGWGLRAGWMAQELLTTFADELGSVTLTPD
                     RTGGVFEVRVDDEVIWSRKERGRFPELKEIKQLVRDRVAPGRSLGHTDAP"
     gene            4142..4324
                     /locus_tag="VM910_22510"
     CDS             4142..4324
                     /locus_tag="VM910_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65330.1"
                     /translation="MQESEVYDYARQLLEAHGDKAVAEAAQKAWALEQQGESENAETW
                     RHIEAALKLMRGPNES"
     gene            4506..>5615
                     /gene="glnA"
                     /locus_tag="VM910_22515"
     CDS             4506..>5615
                     /gene="glnA"
                     /locus_tag="VM910_22515"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HVI65331.1"
                     /translation="MALSCATAQDVLKTIKDENVQMIDLRFTDLPGVWQHFSVPPSAV
                     DADALDEGIGFDGSSIRGFQEIQESDMLVVPDPTTAFLDPFTTATTLVLICNVRDPVT
                     GQTYSRDARYVAEKAETYLKSTGLGDAAYFGPEAEFFVFNEVRYGQGINYAMHEIDSK
                     EGTWNTGTKEAPNLGHKPRQKEGYFPAPPVDSMQDLRSEMVLTMEALGIQIEAHHHEV
                     STGGQAEIDMRFTTLTRMADNLMMYKYVVKNVARKHGMTATFMPKPLFEDNASGMHVH
                     QSIWKGKTNLFHGTEYAELSELGRFYIGGLLKHAWALCGLCAPTTNSYRRLVPGYEAP
                     INLVYSQRNRSACCRIPMYSPNPRAKRVEFRSPDPS"
BASE COUNT         1156 a         1700 c         1718 g         1041 t
ORIGIN      
        1 gcatgagagg ttcccctcga tcacgtttcc cgccaaacca aagcggaaag cagggatgcg
       61 gttcctcttt ttttctctga gggacctggg aaccagcgcg gagttaaaag gttaactcac
      121 attgcactta gcccacatcc aagtagcagg cgcggccgag gaaacgccaa cggcgagcga
      181 aaagcgtatg gcgggccgca atatattgtc tccgtttatg ttttcctcaa aagcaaaagt
      241 cgggaacgcg actcacgatc tgcgcgttgg tagtcggcgc gggacatagg aggaggatcg
      301 tggcagggcc ttgccaccaa ttaacctggt atcgccctga tcggggactg tgccatgtgg
      361 gaatccaatt tgcgtgagtg gaatgtgttt tgtgaatgga ctatcactgc tgatgatacc
      421 gagcgccggg ttgcccgagc cttgagggtt tgcatcggga atgaagttcg gaagctgtac
      481 ggggatgttc cgaacgagcc tatcccctca aaaattgccg acctattgca tcgtctcgat
      541 cagtaaaaca aacccccggc gaccttttgc agatgcggac aggaaagctt ctgaggtccc
      601 taatgagagc cgcagccaac gaatatcgtg ccaaggcgtt tgagtgccta tcgctggccg
      661 aatgcatggc taagcccgag caccgagccg agattctccg atatgctcga tggtggatgc
      721 aattggcgga accggtcgga acttttcgga gtgcttatga ggtggagccc gccctagaac
      781 tagtctagtc ggcagcgggt tgtctcgcgg gcggccctgc aactcaaggg aggcgccgca
      841 ggtcgacaat cgccgccgcg ccagccaccg cctgaagtgg cctactttga caactgcttc
      901 aatgctaagg cgcacagccg ctcggggtcg cgttcgccct ctttcgccag ttcgataatc
      961 agtttagcga ccgccgttgt tgccggatct ttccgatcga ccaatccgag cttgcccaag
     1021 gcggtctcga aggcggccga caagcgcgcg atgtcttccg gcccaaacga atgatcgcgt
     1081 tcgagagatg atcggatagg catgtttcgg ctcctaatag cacatgccgc gcccgtgagc
     1141 ccaagtcttc gccgccggcc agtgctggcc cccgagctca agctgaccaa gcagccgagt
     1201 gccgcgaggc gttgcgcgag ctgagggccg cggtcttggc ccgccatagg gccgacgccg
     1261 agctggctga cctctactgc gagcgagcca accagagcgc gcaggccgcc gagcgcgacc
     1321 cggcgctgct gctgcgatga acgcgggtgc cgcgctacta caagagaccc gcggcggaag
     1381 cgatttcgag taattgaagg tggaaagagt taatccgctc gctcaagaac acattgcagt
     1441 gggtgtttat gctcgccagc aaaagcgaga acatccgcaa cctttctctc ggcctcttcg
     1501 taaggataag tgccgcagat cgcccttccc tcgtagtgaa tgcacagcat ctgcgactgt
     1561 gagaatttcc aaatgtttcc aacccggggg cttcgctttt atttcaaacg cacacttttg
     1621 caaacagcgg cggctgcgcg gcggccgcgc cgccgagaag cgtgatgaac tggcggcgcc
     1681 tcatcactcg atcaccggca tgccatgctc tctgttttgc tcgccaccga tgaaggagtt
     1741 tcatcgcgcg ggctttcggc gtttttgatg cagcgtcacc accgaatgga cgctaggtgg
     1801 ccatgaggcc gcctcgcagc cgcggcacgc tcacgtacaa ccttttgcac tggcggcgga
     1861 atgagagcag cacaccgcgc gtgggcggcc gggggttatc gaaacgtctt ggagtctagc
     1921 agatgcggca aactctcagc agccgcagcc acaacggcag ccacagccgc aacggcagcc
     1981 acaggctctg ccgcatctgc agccgcatct gcagccgcat ctgcagccgc cgcatccttg
     2041 ggcaattctc tcgccgagcc gaggcgtgcc agcgttttcc ttgtcgaaaa cgtagaaggt
     2101 tgccaaactg acgtcggcga tttcttcttc gcttagcgtg atttggggcg gggcagtatt
     2161 ctggggcagc gtatccgcgg tcggcacggc ggtcgcggcg gatgccccgc ccgccaccga
     2221 caaagacacc ccgacgactc cgacaatcgg tacggccacc ttgcgggcct ttcgcttcga
     2281 agcaggcttc ttgtgcggca tagtcgagtc ctcccgtatc ttcgagcaga tgtttgatga
     2341 agtgggccgt acccgtacac ctggattatt cgtacgcttc gcgcagacgc ctcgcaaggc
     2401 cgatgcggcc gcgcgagggc gcggggggag tagttttcaa ggcaaccagg ccgtccgcca
     2461 ccgccgataa aaaaggtcga gcgccgcggc acgccgaatt ggagtgatca gtagacgatc
     2521 tggccgaacc ggtaattcag accgatcttc gcggtatgga tgccgtcgaa gcggtggtcg
     2581 aagcaaaacg gtccgccggc taggatggcc ccggacgggg ttgtcaccgg gccgcaggtc
     2641 tggaacccgc tacgatcgtt gaggccgatg aacatgtact cgcccttgac gctccagctt
     2701 tgggcgaatg cccattcaag tccgccgccg actgtccacg tggtgacagt tttgctgccc
     2761 gaggtcgaga ttagccagtt gccgcatcca atgacggtgt tttggcacgc atcgaggacc
     2821 gaagcctcag ttcgaacgaa agcagcgccg cccttgacgt agagcagcca gcggtcccag
     2881 gcgtatccca atcggccggt taccatgccg taccaatcgc cgaccttcgc gctccccagc
     2941 acgtcaggga ccgtctgcgt gctgatgatg gtacggggat caaaggcgtc gccactcaga
     3001 tgcatgtagc cgccttcggc ttcaaggccg agcacgaagg gcgagttgac cggctgccag
     3061 ttgcagccga gcgtgccgcc gccaatgaca ctgctgccca ggtccgcgtt ccacgagtga
     3121 gcaggtacga gccgcgccgc gacagcgccg cccgaataag acaaaaacgg aggcacggcg
     3181 atcgatccgg ccgggccgat accgttttgg cccttgtcgg tgaatgttgc gccgtcgcct
     3241 ccctgccatg cgccgccgac gtaaccgccg agatagcagc cggtccaact gaaggccgga
     3301 accaccggcg gcgccttccg cacggggaga tcggcggcgg tggcagaact gattgcggcc
     3361 agtgcggcaa ggggtacgca tccgagcaaa agctgtttca tgtgcagccc ccctacaaga
     3421 aaggttgcta agtgtagcac ttcaccatgg gctcgttagt aaccgaagga acacacctca
     3481 aaacaattcg gaaggcgaat cgacgtcaac aagataccgc tcgggccgac ccgtctccga
     3541 gtgcggtcaa tgcccccgcg tttatgagta cacgcctagt cgatagaagc tgcgggcagg
     3601 actattcttg tcgctgctcc tggttgatac cggcgcaccc acggactgca cgcggagggg
     3661 agctatgccg atgagccccg tgatcgagat cacctattgc cgattgtgcg gctggggcct
     3721 gcgggcgggc tggatggcgc aagagctgtt gaccacgttc gccgacgagc tcggctccgt
     3781 gacgctcacc ccggatcgga ccggcggcgt cttcgaggtg cgcgtcgatg acgaggtgat
     3841 ctggtcgcgc aaggagcgcg gccgctttcc ggaactgaaa gagatcaaac agctcgtccg
     3901 cgaccgcgtc gcccccggtc gctcgctcgg gcatacggac gccccgtgac cccatagcgc
     3961 ggacgtggcg ccttgcggcg cattggccct gcatggccgt accattgcat gagcggactc
     4021 ttgccgtcgc gacgtgctaa gatgcgcctg caggtcttga tgggtgtcaa cgtccaccgt
     4081 tctcgggcgc gcgatggggg tggagaggcg cgctgaagac aaggagcatg acgccatgag
     4141 aatgcaggaa agcgaagtct acgactacgc gcggcaattg ttggaggcac atggcgacaa
     4201 ggccgttgcc gaggccgcgc aaaaggcttg ggccctggaa cagcagggcg aaagcgaaaa
     4261 cgccgaaacg tggcggcaca tcgaagcggc cctcaagctg atgcgcgggc caaacgaaag
     4321 ctgaaagtcg cggccacagg cgacaggcct tgcttatagg agcgtcactc gcggcgtgac
     4381 cgttgaaacg gccagcttgc cgccgacgtc gcggccgtga cgttcgcact tgactgcttg
     4441 gtggggtttg atcagcgctg cagcaatcat ccaacacaac cgctttcaga cgcgggagga
     4501 aactcatggc tctcagttgt gccacggccc aggatgtcct caagacgatc aaggacgaga
     4561 acgtccaaat gatcgatttg cgatttaccg acttgcccgg cgtctggcag catttttccg
     4621 tcccgcccag cgcggtggac gccgatgcac tcgacgaggg catcggcttc gacggctctt
     4681 cgatacgcgg gtttcaggag atccaggaaa gcgacatgct ggtcgtcccg gacccgacca
     4741 cggcgttcct cgacccgttc accacggcga caacgctggt cttgatttgc aacgtcaggg
     4801 atcccgtcac cgggcaaacc tatagtcgcg acgctcgcta cgttgccgag aaggccgaga
     4861 cctacctgaa gagtaccggc cttggcgatg ctgcctattt cggcccggag gcggagttct
     4921 tcgtcttcaa cgaggtgcgc tacgggcagg gcataaatta cgccatgcac gaaatcgatt
     4981 cgaaggaagg cacctggaac accggcacga aggaggcgcc caacctgggc cacaagcctc
     5041 gccagaaaga agggtatttt ccggctcctc ccgtcgacag catgcaggat ctgcgcagcg
     5101 agatggtgtt gaccatggag gcactcggca tccagatcga ggcccaccac cacgaggtct
     5161 cgaccggtgg tcaggcggaa atcgacatgc gtttcaccac gctgacacgc atggccgaca
     5221 atctgatgat gtacaaatac gtggtcaaga acgtggcccg caagcacggc atgaccgcaa
     5281 ctttcatgcc caaaccgctg tttgaagaca acgcctcggg catgcatgtc catcaaagca
     5341 tttggaaagg gaaaaccaac ctcttccacg gcacggaata tgccgagctc agcgagcttg
     5401 gccgctttta tatcggcggc ttgctgaaac acgcctgggc actatgcggg ctttgcgctc
     5461 cgaccacgaa ctcgtatcgt cgcctagtgc cgggttacga ggcgccgatc aacctagttt
     5521 attcgcagcg caaccgctcc gcctgctgca ggattccgat gtattcgcca aacccgcgtg
     5581 caaagcgggt cgagtttcgt tcgccggacc cctcg
//
LOCUS       DATLJT010000977         2986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_295787,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000977 DATLJT010000000
VERSION     DATLJT010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295787"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..732
                     /locus_tag="VM910_22520"
     CDS             382..732
                     /locus_tag="VM910_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65332.1"
                     /translation="MNYRQPELTLEDILSDSIIEAVMKADGVDPHQLAAMLKELGRRW
                     AGERPNVAQGRHNPTPGRCEHATPGAPSPSRNSFRRRYSMADDRFDWLILLVAGAIAS
                     VLLLAMTAYNAIAG"
     gene            1114..1467
                     /locus_tag="VM910_22525"
     CDS             1114..1467
                     /locus_tag="VM910_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65333.1"
                     /translation="MGYGFLTVEAQWYAARWSLGVSGLIMDGVRPARVADHIIDDLRK
                     RERDELIKLPKRPALQPGDQVRILQGPFAGQLGLYAGMRPHERVLVLLQLLGGQQEWS
                     SRRAASRQRAGGPAS"
     gene            complement(1726..2295)
                     /locus_tag="VM910_22530"
     CDS             complement(1726..2295)
                     /locus_tag="VM910_22530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65334.1"
                     /translation="MKRSDVLVIGLLAAAMSASTPAAAQDRYPLRPVRLVIPSVPAGV
                     HDVIGRVWAERVKPQFGAIVIDNRGGGAGSIGANDVAHSAPDGYSILLGSTTTHVLVP
                     PVMANPPYDPVKDFSAVAVFAYSSSIVVNPSVPVRTLAELIAYAKANPGKLSYGSAGT
                     GSITHIAGEYFKRLAGGLEIVAVPYKSFG"
     gene            complement(2435..>2986)
                     /locus_tag="VM910_22535"
     CDS             complement(2435..>2986)
                     /locus_tag="VM910_22535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65335.1"
                     /translation="AYAKARPGQLSFASVGPGVPHHLYAELLMSTAGIAMTHVPYKGS
                     APALNDVVAGHIPLMFCDFAPAVGMLRAGKVRPLGVTTRTRVPAFPDIPPIAEAGLPE
                     FGALPGWHMLVAPGKTPRDIVAKLHGELLDILALPEIETEILRLGMLPFENRSVEGLQ
                     DFVKSETVRWGKVVQQAGIAGSE"
BASE COUNT          568 a          928 c          943 g          547 t
ORIGIN      
        1 gtggtgcgct gctaatcgcg agactcgatc ggatgtgccg cgctgatgtg gccgtcgcgg
       61 aaacggcacg ggcggcgacg actccgagct gctccctgcg tggagcgctc catcattgac
      121 gcgcgtccac gcaggtagga atgaacgcgc tgacctgccg cttggcccca cggcatggcc
      181 aacaccgtca cgatccccaa ggcatggttc taagcccttg cgttagctga cgattaacgg
      241 gatggcgcgc cggcgccgtc aaaatctcca ggaaatctcc aaagaaattc aagacggact
      301 gcaggtgggc ttcaagcctg acacctcgtc tgcgtttagg cactccccaa ccccggttcg
      361 cgaccagcga ggaaaggaat gatgaattat cgtcagcccg aactaacact cgaagacatc
      421 ctgtccgatt cgatcatcga ggcagtgatg aaggccgacg gtgtcgatcc acaccagctt
      481 gcagcaatgc tgaaggagtt gggacgaagg tgggctgggg aacgcccgaa tgttgctcaa
      541 ggacggcaca atccaacacc cggcagatgc gaacacgcga cccccggtgc cccttctcca
      601 tctagaaatt catttcgacg gagatattcg atggccgacg atcgatttga ttggctcatt
      661 cttctcgttg ccggggccat tgccagcgtc ttgttactgg ctatgaccgc atataacgcg
      721 attgcaggct agggtagttc aacatccata acgccaccct cttcgggaca atgcccctgg
      781 accccctgaa cgcacatacc tggtcctctg acaccgcgcg acgcgggttc catgattata
      841 ttatggccat gccccgagcg cgctgactgg gccgcctacg tgctaccttt ggcccatgag
      901 cgcccatgag ggtcagctcg gtaacctgac ccagcaaggc cacttgctcc gagcggcgca
      961 agtggcctca gtctacggga ccaaggcacc agggtatctc aacaggcggc caacgagcca
     1021 ccaggcttac cgtcataccc ggctcgaagg tgccatgcga accttctcag gggccgtcca
     1081 gcgtgatgat ctcaacgcga ttggccgccc ctcttggggt acggtttcct taccgtcgag
     1141 gcacagtggt acgccgcccg ctggagcctc ggcgttagcg gtctgatcat ggacggtgtc
     1201 cgcccggcgc gcgtcgctga tcacatcatc gatgacctac gcaagcgcga gcgcgacgag
     1261 ctgatcaagc tgcccaagcg gccagcgcta cagccgggtg atcaggtgcg gatcctgcaa
     1321 ggcccgttcg cggggcagct tggcctctat gctggaatgc gcccccacga gcgcgtcctg
     1381 gtgctgctgc agctgctcgg cggtcagcag gagtggagct cgcgaagggc agcatcgagg
     1441 caacgggcgg gaggtcccgc ttcctaggtg cttcctaggt cgatttttag gaagcaagac
     1501 aaactcacaa gcgtccgttt tttcagcggt ttctcggcgc gtattgggcc ctctctgcgt
     1561 tgacgaagcg tgcgcgaggg tctgccgcga agtggcggcg gccgcatgcg gggaaatacg
     1621 cgcgctaggc gcctcaaacg ctcaccggga cactaacggg acactcacgc ggcctcgctt
     1681 tttcaagcca ttggtagctt tgcgatttct gcaaccgatc tcgggttagc cgaagctctt
     1741 gtagggcacc gccacgatct caaggccgcc ggccaatcgc ttgaaatatt caccggcgat
     1801 atgggtgatc gagccggtgc cggccgaccc ataagacagc ttgcccggat tggccttggc
     1861 gtaggcgatc aactccgcaa gcgtgcgcac cggcaccgac ggattgacca cgatcgagga
     1921 gctgtaggcg aacacggcga cggcggaaaa atccttgacc gggtcgtagg gcggattggc
     1981 catgaccggc gggaccagga cgtgggtggt cgtgctgccg agcagaatgc tgtagccgtc
     2041 gggcgctgaa tgcgcgacgt cgttggcccc gatcgaaccg gcgccgccgc cgcggttgtc
     2101 gatgacgatg gcgccgaatt gcggcttgac ccgctccgcc catacgcggc cgatgacgtc
     2161 gtgcacgccg gccggaaccg acgggatgac gagccgaacc gggcggagag gataacggtc
     2221 ttgggccgcc gccggcgtcg atgcgctcat cgctgccgcg agcaatccga tgaccagcac
     2281 gtccgaccgc ttcacggcgt tcctcgtctt attgttggtg ttgccttccc cccgcggcgc
     2341 ggatgttagt gaatgtggcg ggcggtgcca ataatccaat gccggcggga cagtggtggc
     2401 gagacgaacg ccagtggctc agcgacgcgg ccaactattc cgatcccgcg atcccggctt
     2461 gctgcacgac cttgccccag cgcacggtct ccgacttgac gaaatcttgc agcccttcga
     2521 ccgaccggtt ttcaaaggga agcatgccga gtctgagaat ctcggtctcg atttccggca
     2581 gcgctagaat gtcgaggagc tcgccatgaa gctttgcgac gatgtcgcgc ggcgtcttgc
     2641 cgggcgcgac cagcatgtgc catcccggca atgctccgaa ttcgggtaga ccggcttccg
     2701 cgatcggcgg aatgtccggg aacgccggga cgcgggtcct ggtggtgacg ccgaggggac
     2761 gaaccttgcc agcccgcaac attccgacgg ccggcgcaaa gtcgcagaac atcagcggaa
     2821 tatgtccggc aacgacgtcg ttcagggcgg gtgcgctgcc tttgtaggga acgtgcgtca
     2881 tcgcgatgcc ggcggtgctc atcaagagct cggcgtagag atgatgcggc acgcccggcc
     2941 cgaccgatgc gaacgagagc tgcccgggtc gcgcctttgc gtatgc
//
LOCUS       DATLJT010000978        11716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_983450,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000978 DATLJT010000000
VERSION     DATLJT010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983450"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VM910_22540"
     CDS             complement(<1..166)
                     /locus_tag="VM910_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVI65336.1"
                     /translation="MPTFKAIVVEKGDGGQKVRLTDFDEANLMDGDVTVRVEWSTVNY
                     KDGLAITGKAP"
     gene            complement(604..1335)
                     /locus_tag="VM910_22545"
     CDS             complement(604..1335)
                     /locus_tag="VM910_22545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI65337.1"
                     /translation="MSELGTLVLTISIFLGGVVSGFSGFAFSAVSGVVLLHFLEPLVA
                     IPLMMCCSIASQISSLVAIRQFVQWRESAPLLIGGAGGIPIALYVLTLTNAHVFRILV
                     GIFLATYAIYMLTKPACGIFRQAASPVIHTLVGFAGGLVGGLTAMPGALPIVWCDLRG
                     VSKEHQRGEVQPFILIMQAFAIILLICTPGTLPADLLPVLPLAIPTVVAGTLVGILLW
                     GKIDDAKFRYSVLSMLLIAGLILIR"
     gene            1588..1755
                     /locus_tag="VM910_22550"
     CDS             1588..1755
                     /locus_tag="VM910_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65338.1"
                     /translation="MPPFGYFSLIRFERLPRVRTGEAGQERMVGGWNRQGEVRILANM
                     FFPEVYDLEGD"
     gene            1798..2619
                     /locus_tag="VM910_22555"
     CDS             1798..2619
                     /locus_tag="VM910_22555"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI65339.1"
                     /translation="MKIENAVPYLGALVTGLDVKRMTDAEWKTLYQTWLDRHVLIVRG
                     QELSKADFLAYSRRFGRLKPHLVKHTRDPENPEITLMGVTSMAKDTNGEKLILDRGQG
                     WHTDSPWDTEICKGTQLYGIEIPSYGGDTLFASMYAAYDALPERLKKRIAPLKVEFRY
                     GGRERKWFDLLDPEDQARPPAVHDMVRVHPETGRRSLYVNPTHVIRIVGMPEAEGDRL
                     LAELYWYMLQPNAEYRHKWKTGDLVIWDNRCTVHSAAGGYPSDERRIHWRTTIIE"
     gene            complement(2800..3528)
                     /locus_tag="VM910_22560"
     CDS             complement(2800..3528)
                     /locus_tag="VM910_22560"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HVI65340.1"
                     /translation="MATVEIVDRSTMIEKLRDGLVLRPRETAIVAIDMHRGHLDPKVA
                     TMAAKPDDAKRVIAAAKELLGLARATGVPVIHVKLVFRKIPGIGSEGMTQPFWHALHE
                     MLTEEDRLTPGRRSTVDGHNIVGSPGTELIPELYAEGDYVIDNKKRLDCFHGTDLKML
                     LDVLRVKNVCLMGINTNTCVLNTAFTAFNNDYRVVVLSDCVASMYGDDLHEFGLQNIA
                     RCLGWALGNEQFREKIEQGAARND"
     gene            complement(4980..7808)
                     /locus_tag="VM910_22565"
     CDS             complement(4980..7808)
                     /locus_tag="VM910_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65341.1"
                     /translation="MFEEFDARSQTKVVRDAAGTARTLSHANRYVVTGAPTPQLAARE
                     YLHRYGELLGLRPEQLGHLFLPAEQEPGAGGVEYRLLSEKHQFDLTTVAFYQTCFGLP
                     VWEAGLSITMKANPLRVVGAQSTLHPDLEVKRPPARAIARLKTLDAESLASSLGLAGR
                     ARHDAPLIDSQRFMIYRYDAARRTDGSARPQSEELVFQAGGPGLPLPPVDSSIRDGRH
                     CVVLAVYFRFDVPPFRPLHWVALVEVETLSALLLRPFVDSVSGLVFQADPVTLAGGPT
                     ANANSAALNPLRSSVTLAGLAPPVNGAQALSGSNVTLSDVETPTVPPPTESVGTNFDF
                     DARTNDFAAVNAYYHCDRFFRLVEDLGFSVASYFPGTTFPTAVDHRGHFSPTHLQGDE
                     INAHCAGTAGGTGILYTGFSLADTADLANPIGIACDWRVVMHELFGHGALYNHIGAPR
                     FQFSHSAGDSFAAILSDPDTQAPDRFATLPWIVGIPPAFRRRHDRTVAAGFGWSGDIA
                     LHPFDPVKDGAGYNNEQILSTTMFRVYRSIGGDSADIATKRFAARFTCHIMLAAIKTL
                     TSATSPPDAASFAGALMQADRGDWPSEGQSGGAYNKVIRWAFEKQGLYQPAGTATPNN
                     NEGAPPPVDVYIEDGRNGEYQFQSNHWSCQAIWNRRHNDGGVSHEEPIVGVTNYAYVK
                     IKNRGSETATNVVVRAFHCRPSAGLVYPDDWQAMTTPQLAAANVPSNSAAEILVGPFE
                     WVPSQVGHECMLMVASAAGDPSNVDNFTVGDSIPDWRLVPNDNNIGQRNVHPVPSSTA
                     RRLTNSFDRMSFQLKNPLTRRARMSVKAVLPPLLDKRGWRIAFANAGADAFTLEPGGG
                     REVIMRLRLGRQFSGNEIRKAKNRTIEIEAYADGILVGGMSYPLLYDAKKSAGGTRAS
                     RSSAKSRTSPKAKSRPAAKKIRQPGAHR"
     gene            complement(8496..>11716)
                     /locus_tag="VM910_22570"
     CDS             complement(8496..>11716)
                     /locus_tag="VM910_22570"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HVI65342.1"
                     /translation="RSAPSTFSVPFSCLPHLLERQAKRIPGAPAILALGRAPLTYGRL
                     YQQVDKVGHALRAMGIGRHDRIAVVLPNGPEMAVAILTVASSAACAPMNPAYGAEELD
                     RYFADLRPRALITQAGIDSPARRVALSRGIRVIELSATFDAEAGVFTLEGRHWRMRSH
                     ESVSGGDVALLLFTSGTTSQPKIVPLTQVNICTSAYSSAAAVALGETDRCLNVLPLFH
                     GHGLIATVLASLAAGASVVCTPGCDVNSFFAWLTAFRPTWYSAVPTMHQAILAQARHA
                     REQAADCRLRLVRSASAPLPPRVFADLERTFETSVIEFYGMTETASSPVACNPLPPRQ
                     RKPGSVGLPVGLDVGIMDEGGALLHDGQIGQVVVRGASVMPGYDRDPTATRAAFAGDW
                     FKTGDRGFFDADGYLFLAGRTQEIINRGGEKIAPREVDEVLLEHPAVAEAVTFAVPHA
                     TLGEDVASAVVLRPQAAATPKDIRQFTVGRIADFKVPRQVLIVGELPKGSTGKVQRVG
                     LAAKLGLASQAMPQTFVAPRTPLEKELVALWAETLQVERVGIHDDFFASGGDSLLASR
                     VLTYMHQSMHLKVEVSRFFEAPTVAEMAGHLETLIQTDQARPLSSAIVPIVREDGLPA
                     SFAQERLWKLQHALPGLPFFNILYALRLTSPFDMAVLERSINEIVRRHEILRTTFAAA
                     DGRYVQVIAPQLTVPLTFNDLHALSKSKKERVAHQLIQDEVLHSFDLAQGPLFRTGLV
                     RLAEREHLLLFTMHQVISDGWSLGVLVKELAALYDAFSTRKASPLPPPSTQYADFAHW
                     QRHWQSHPDIVAQLAYWREQLHGPLPLMKLANARLRRTIDGFRTARREVAVPPSLSEG
                     VKRFSHREGGTLFMALVAGLKTLLHRYVGQDDLRVATNVANRNRPGTEELIGPLANTV
                     ILRTNLGGDPSLREVMRRVRATTLAAFAHQDLPFEQLAEILEHEHAIKPAALAQVMIL
                     LQNATLRPVAGSADTLTFEEANPSMLLPLVTLTTFDVILMLRESAHGLVGCCVYNPHL
                     FRAMTIDRLLRDFQGVLKHMVTQPERPISAISVSLNEKPSIR"
BASE COUNT         2337 a         3399 c         3648 g         2332 t
ORIGIN      
        1 cgggtgcttt gccggtgatc gcgagcccgt ccttgtaatt caccgtcgac cattcgacgc
       61 ggacggtgac atcgccgtcc atcagattgg cttcgtcgaa atcggtgagg cggacctttt
      121 gcccgccgtc acccttctcg accacaattg ccttgaatgt cggcacgtat cccctccatt
      181 catacgtgcg aaagctgtac cgtgcggcgc cggccgccgc aactcgatgt ctggaatcag
      241 cgcctttgag accgagtctt gcgagtgatc ttttggtccg aggccgttgg tcaacacgct
      301 gcttgctgag tcacgcacat gaatccagcc gctgcgcacg gcgcctttct cgacggtcct
      361 caaagctgtg gcaagcgagt gtccgaggga tatcccatgt tctgggtgtg attttcctgg
      421 atcagcacgt tcatacgatc agcatctcga gttcgcgcgc catttcgtcc cgcttgaggg
      481 aatcaatcgc gactaagaca tgccgctgcg tgtgcgccag gaaattaccg catattttca
      541 atctcgattt cgccaacaag atcttggcag gagcgccgga ggctggccgg cgacggtggc
      601 ggtttagcgg atcaagatca agcccgcgat caacagcatc gacaagacgg aatagcggaa
      661 tttggcgtcg tcgatcttgc cccacagcaa aatgccgacg agcgtgccag cgaccacggt
      721 gggaatagcc agtgggagaa ccgggagaag gtccgcagga agggtgccgg gcgtacaaat
      781 gagcaggata atggcaaagg cctgcatgat cagaataaac ggctgaacct cgcccctttg
      841 gtgttctttt gaaacacctc gcaggtcgca ccaaacgata ggcaatgcgc ctggcatcgc
      901 ggtcagaccg ccgactaggc ctcctgcaaa gcccaccagt gtatgaatga cggggctggc
      961 ggcctggcgg aatattccgc acgctggttt ggtgagcatg tagatggcat aggtcgcaag
     1021 aaagatgccg accaagattc ggaacacgtg agcatttgta agggtcagga cgtacagggc
     1081 gatcggtatt ccgccggcac ccccgataag caaaggcgcg ctctcgcgcc attggacaaa
     1141 ctgacgaatg gccaccaaag agctaatttg ggacgcgatg ctgcaacaca tcattagggg
     1201 aatggccacc aacggctcaa ggaaatgcag cagaacgacg cctgacacgg cggaaaaagc
     1261 aaaaccggaa aaacctgaga cgacaccgcc caggaagatc gagatcgtga gtaccagggt
     1321 gcctaattcg ctcattattc agcatgaaat cttgagaacc gtcacgcgcc gacacgtcag
     1381 caggattgcc tcagatggta taccggacgc cgcattcgga agtgtgaggt aactcactat
     1441 cgcgatccgg cgctgtcctg cccacgcggg gtcgcatcac cgcaattcag aggcttgccc
     1501 aggcgcggcg gctcggctga cggccggaat cccccgggca gcggcgggcg caatgggctg
     1561 atcgctgcgc gcgctcgagc gccgccgatg ccgcccttcg gatacttctc gctgatccgg
     1621 tttgagcggc tgccgcgggt gcgaacgggc gaggcagggc aggagcggat ggtgggcggc
     1681 tggaatcgtc aaggcgaagt ccgtatcctg gcaaatatgt tctttccgga ggtgtacgat
     1741 ctcgaaggcg attgagatga caggcatgcc acacccagcg atgaaagcag gcagcgcatg
     1801 aaaatcgaga atgcagttcc ctacctcggc gcgcttgtga ccggcctcga cgtcaagcgt
     1861 atgacggacg ccgaatggaa gaccctgtat cagacgtggc tcgatcggca cgtgctgatc
     1921 gtgcgcgggc aggagctgtc gaaggccgac tttctagcct atagtcgcag gtttgggcgc
     1981 ctgaagcccc acctggtcaa gcatacgcgc gatcccgaga accccgagat cacgctgatg
     2041 ggcgtgacga gcatggccaa ggatacgaac ggcgagaagc tgattctcga tcgcgggcag
     2101 ggctggcata cggacagtcc atgggacacg gagatctgca agggtacaca gctctatggg
     2161 attgagattc cgagctacgg cggcgacact ctgtttgcgt ccatgtatgc ggcttatgac
     2221 gcgctgcctg agcgcctgaa aaagcgcatt gcaccgctca aggtcgagtt ccgttacggt
     2281 ggacgcgagc gcaagtggtt cgatttgctc gaccccgagg atcaggcgcg gccgcctgca
     2341 gtgcatgaca tggtgcgcgt tcaccccgag acggggcgaa gatcgctcta tgtcaatccg
     2401 acccatgtca tccgcatcgt cgggatgcct gaggccgaag gcgaccggct gctcgccgag
     2461 ctctactggt acatgctgca gcccaacgcc gagtaccggc acaagtggaa gaccggtgac
     2521 ctcgtcatct gggacaaccg ctgcaccgtg cattcggctg ctggagggta tccgtcggac
     2581 gaacgtcgga tccattggcg taccacgatc atcgagtgag cgcgatcaaa agttgactag
     2641 gaatctcgtg accatgcgcg agacgcgccc acccatgggt cgcagttgcc ggcgcctcta
     2701 aaagacgacc ctacgggaac gcggctaaac ctttgcaaaa aatggacgcg cttgaggcga
     2761 tttggttgtt gaggcgatcc aaattcttgg tgacaacagt cagtcgttgc gcgcggcgcc
     2821 ctgttcgatc ttttctcgaa actgttcgtt gccgagtgcc cagccgaggc aacgcgcgat
     2881 gttctggagt ccgaactcat gcagatcatc gccatacatg cttgcgacac agtccgacaa
     2941 cacaacgacg cggtaatcat tattaaaagc cgtaaacgcc gtgttgagta cgcaagtgtt
     3001 ggtgttgata cccatcaagc atacgttttt gacgcggaga acgtccaaga gcatctttag
     3061 atcagtgcca tggaagcagt cgagacgctt cttgttgtcg atgacgtagt cgccttctgc
     3121 gtatagctca gggattagct cggtgccggg cgagccgacg atattgtggc cgtcaacggt
     3181 cgagcggcgg cctggggtga gccgatcttc ctctgtgagc atttcgtgca acgcgtgcca
     3241 gaatggctgg gtcatgccct cgctgccaat tcctggaatt ttccggaata cgagcttcac
     3301 gtgaatgacc ggcacgccag ttgcgcgagc aagtcccaga agctccttgg cggcggcaat
     3361 gacccgtttg gcgtcgtctg gcttcgccgc catcgtggcg actttggggt cgaggtggcc
     3421 ccggtgcata tcgatcgcca caatcgcggt ttcacgcggc ctcagcacga gcccatcgcg
     3481 caatttttcg atcatggtcg agcgatcgac gatttcgact gtggccatga ttgcctccca
     3541 gccttcgacg gcgccgaagc cgaagcgcca catcgtgccg cacgggcccg gtgctgacaa
     3601 cctatttcgc ggaacgccta ctggtgagcc gacccacgtc cggctttgtc ccgggcgggc
     3661 ggatgggctc cgtcgatggg aaagggctcg gacgcccgta tcattggtcg cactgccggg
     3721 gagggtcttg ccgtctgcac gatcccgggc cagacaggaa gcaactgcat tcgtcctgac
     3781 ggacctgcac ctgcttgccg tcaaatctgc atcccgtcat aagcgctagt ggatcactca
     3841 ggtgccgcaa ccccatccta tgtccaaact cgtgcggcca caggatgctg ggaaacggat
     3901 ccgccggatg tcgaacgaca attatcgatc gactccccgc acgaggtggc catgcgcatc
     3961 cgtcgaaact atttcccatg ttaggtcggc agaacctaat ctcctcgaca atcttaacgt
     4021 cgccatccac gctatggaca gcatcgcgct gggccctggt ctcaatgact tttggcgtat
     4081 cggccgatcc gaaggtgccg attggaccat cccgtttgaa tgtcacgttg catgaactct
     4141 tcgtcaggat ttccgatgcg tcgtgcaaga tactgtcaac ttcgtgctcg cccagcgcca
     4201 ccacgttcac gtgacggctg accttcaacg agtacgggct ctgcgcgctg gccggctcgg
     4261 cccacatcag cagacccagg cacacgatcg caataatggc ttgcattttc accctcgtgg
     4321 cgcgcggatc gtcgcacttc gtgccaactc tttactgcca cgcagcagcg ccgatccgga
     4381 gcttatttgg acttcaatcg gttgtacgtg cgacgcagtt tcgttctcaa tgccctccgg
     4441 atctttggat gctctgtcag gtaccttggc tgcgtgttga ttatcttgtg gatctctgga
     4501 gatgttcgca ggtgccgtag agcttctttt ttgagggccg cccgctgtct cttgctgagc
     4561 ccctttagtt ccttctcgga atatccgccg attggatgtc ccatgggatg tatctcctct
     4621 agaactcgcg ttgatgaact agaccatgta gaaaacgccg ggcacgcagt ccacggattc
     4681 ggtggtttcc catccagcga aacgttcctc tcttcgaaca aacgcggatt gcagcgattg
     4741 gtcgctccgg ctgtattagc atccgttcga gtattccggg cagtctcgaa gcgggcaatc
     4801 gatcgtcctg acgccgaaga gatgcagctg acagttaccg tacccccgag gccatgggcg
     4861 ctttcgcgcc gtatcaagat aacctcgaat attgttatcg tcactatggg caacggcatg
     4921 atcatgactg cgaagcgctc atatcatcgg ggccggcaac tgcccgtcgg tgaattcgat
     4981 cagcggtgag cacctggctg ccgtattttt ttcgcggccg ggcgcgactt tgccttcggg
     5041 gacgtgcgcg acttcgcgct gctgcgcgat gcgcgcgtgc caccggctga cttctttgcg
     5101 tcgtacagga gcgggtagga cattccgcca actaaaatcc catcggcata ggcttcgatt
     5161 tcgattgtcc gattcttggc tttgcggatt tcgttgccgc tgaattgccg gcccagacgg
     5221 agcctcatga ttacctcccg cccgccgccc ggctcaagcg taaacgcgtc agcacccgca
     5281 tttgcaaatg cgatccgcca gccgcgctta tcgagcaaag gaggcaggac tgccttgacg
     5341 ctcatgcgcg ctcgcctggt caaggggttc ttgagctgga agctcattcg gtcgaagctg
     5401 tttgtcagtc gcctcgcggt gctgctcggc acggggtgga cgttgcgctg gccgatattg
     5461 ttgtcgttcg gaaccagccg ccagtcggga atcgagtcgc ctacggtgaa gttgtcgacg
     5521 ttgctcggat cgcccgccgc cgaggcgacc atgagcatgc attcgtgtcc tacctgcgag
     5581 ggcacccatt cgaacggacc gacgagaatt tcggcggccg agttggacgg tacgttcgcg
     5641 gctgcaagct gcggcgtagt catcgcttgc cagtcgtcgg gatagacgag ccccgcggac
     5701 ggccggcagt gaaaggcccg cacgacgaca ttagtcgcag tctccgagcc gcgatttttg
     5761 attttcacgt atgcatagtt ggtgacgcca acgatcggct cctcgtggct cacgccgccg
     5821 tcattgtggc gccggttcca gattgcctga cagctccaat gattggactg gaactggtac
     5881 tcgccgttac ggccgtcttc gatgtacaca tcgaccggcg gcggcgcgcc ctcgttgttg
     5941 ttcggggtcg cagtgccggc gggctgatat aggccttgtt tctcgaacgc ccagcgaatc
     6001 accttgttgt aggcgccgcc cgactgaccc tcggagggcc agtccccgcg atcggcctgc
     6061 atcagcgccc cggcaaagct tgcggcatcc ggagggctgg tggccgaggt aagggttttg
     6121 atggcggcga gcatgatgtg gcaggtgaag cgggccgcaa aacgcttcgt cgcgatgtca
     6181 gcggaatcgc ccccgatcga ccgatagacg cggaacatcg tggtcgaaag gatctgttcg
     6241 ttgttgtagc ctgctccgtc tttcacggga tcaaatggat ggagcgcgat atcgccgctc
     6301 cagccgaagc ccgcggcgac cgtccggtca tgacgacgtc ggaaggccgg gggaatgccg
     6361 acaatccacg gcaacgtggc gaagcggtcc ggcgcttggg tgtccgggtc actgaggatc
     6421 gccgcgaagc tgtccccggc actgtgagaa aactgaaagc gcggcgcgcc gatgtgattg
     6481 tacagggcac cgtgcccgaa aagctcatgc atgactacac gccaatcgca ggcgatgccg
     6541 atcggattgg cgagatcggc ggtgtccgca agcgagaagc cggtatagag gatgcctgtt
     6601 ccgccggcgg ttccggcgca atgcgcattg atttcgtcgc cttggagatg agttgggctg
     6661 aaatggccgc gatggtcgac cgcggtcgga aatgtcgtgc cggggaaata gctggcaacg
     6721 gaaaacccga ggtcctccac caaccgaaag aaacggtcgc aatgataata ggcgttgacc
     6781 gcggcgaaat cgttggttcg cgcatcgaag tcgaaattgg ttcccaccga ttcggtcggt
     6841 ggcggcacgg tcggagtttc cacatcggaa agcgtcacgt tgcttccgct caaggcctgc
     6901 gcgccgttca cgggcggggc gaggcctgcg agcgtgactg agcttcgcaa cgggttgagc
     6961 gcagcgctgt tggcgtttgc cgtcggaccg cccgcgagag tgaccggatc ggcctggaac
     7021 accaaaccgc tgacgctgtc gacgaatgga cgcagaagca gcgcagatag ggtctcgacc
     7081 tcgaccagcg caacccagtg caacggccga aatggcggaa cgtcgaaacg aaagtagacg
     7141 gcgaggacga cgcaatggcg gccgtctcgg atcgaagaat cgaccggggg cagcgggagg
     7201 ccgggaccgc cagcctgaaa tacgagctct tcgctctgtg gtctagccga tccgtccgtg
     7261 cgcctagccg cgtcgtatcg atagatcatg aagcgttgac tatcgatgag cggtgcatcg
     7321 tgacgcgctc tgccggcgag accaagactg ctggccaagc tctccgcgtc gagggttttc
     7381 aatctcgcaa tggcccgcgc gggtggccgc ttcacctcaa gatcagggtg cagcgtcgac
     7441 tgtgcgccga ccacgcgtaa tgggttggcc ttcattgtga tcgagagccc tgcttcccag
     7501 accggcaggc cgaaacaggt ttggtagaac gcgacggtgg tcaggtcgaa ctgatgtttc
     7561 tcgctaagga ggcggtattc gactccgccg gcgcccggct cctgttcggc cgggaggaag
     7621 agatgtccca gctgctccgg ccgcagcccc agcaactcac cgtagcggtg tagatactcg
     7681 cgtgccgcga gctggggtgt cggtgccccg gtgacgacgt agcgattggc gtgggaaagc
     7741 gtacgcgcag tgccggccgc atcgcgcacg accttggtct gtgaccgtgc atcgaattcc
     7801 tcgaacatgg tttccttccc atttgcattt gttggcgtgc gtctcaaacg gctggtcgtc
     7861 acctccagcg ttcgccaatg agtgcagcgc gagatcagcg cgacactctg gacagtcgct
     7921 gttcatcaga tcccgatgtc ggatctcgat accggaaagg cattgtgggc ggtgtgagct
     7981 ggttcacacc agacgctttt tccgcactat gtgttaggtt ggtttggacc tgttagggaa
     8041 ttggcttgga cgggcaacgg aaatggtcgt ttggtggcaa caccttctgt cactggaagg
     8101 ggttcttcgt gccggcatag gatagctaat ggcgcggccg tggtcttgcg gaggccgcac
     8161 cattagctgc tcttggcggt gatctcggcc ggtgggtctt gccctggcgg cgctggccag
     8221 gagcctacgt cacttcttct tccgccgcgg gcgagtctta cgcttcttag agatcaattg
     8281 attgtataca ggaagggttt tatttctgag caacctgcga atctgtgcag aagtctggat
     8341 ttggtgtaga actgcgtctc tgaggatttc gcgttggcgc tcgttcagca cctcaagctc
     8401 ctcgcaagga tagactgggg gaaatattcc catggcggac ctctctgggg ttgggactga
     8461 gggatcggtg gtggggacaa agctcaggca aacgcttatc gaattgacgg tttctcattc
     8521 agcgaaacac tgattgcgga gattggtctc tccggctgcg tcaccatatg tttgagcacc
     8581 ccctggaagt ctcgaagcag gcgatcgatg gtcatggcgc ggaagaggtg cggattgtag
     8641 acgcagcatc ccacgagccc atgggcgctt tcgcgcagca tcaagataac atcgaatgtt
     8701 gttagcgtca ccaggggcaa cagcatgctc ggattggcct cctcgaaggt gagcgtgtct
     8761 gcggagccgg ctacaggccg caacgtggca ttctgcaaca aaatcataac ttgagccagc
     8821 gccgcaggct tgatggcgtg ctcgtgctcg agaatctcgg caagctgttc gaaaggaaga
     8881 tcctggtggg cgaaagctgc gagggttgtt gcacgaaccc gacgcatcac ttcccgaaga
     8941 ctgggatcgc cgccgagatt ggtgcgaaga atcacggtat tggccaacgg gccgataagt
     9001 tcttcggtcc ccggacgatt gcggttggcg acgtttgtgg ccacgcgcaa gtcgtcttgg
     9061 ccgacgtagc gatgcagcag cgtcttcaag ccggcgacga gcgccatgaa cagcgtgccg
     9121 ccttctcgat ggctgaagcg tttgacgccc tcggacaggc ttggcggcac cgcgacctcc
     9181 cgccgcgccg tgcggaaacc gtcgattgtg cgtctcagac gggcgttggc gagcttcatc
     9241 aggggcaatg gaccatgaag ttgctctcgc cagtatgcga gttgcgcaac gatgtccgga
     9301 tgcgactgcc agtgccgttg ccagtgtgca aaatcggcgt actgagttga aggcggcggc
     9361 aggggcgacg cctttcgggt cgagaaagca tcgtataaag cggcaagctc tttgacgagc
     9421 acgcccagcg accagccgtc gctgatgacc tggtgcatgg tgaagagcaa gaggtgttcc
     9481 cgctcggcca ggcgcacgag gccagttcga aacagcggac cttgcgcgag gtcgaaggaa
     9541 tgaagcactt cgtcttgaat gagttggtgt gcgacgcgct ccttcttgga tttggacagc
     9601 gcgtgcaaat cgttgaacgt caggggcacg gtcaactgcg gggcaatgac ttgcacgtat
     9661 cgaccgtcgg cggcggcgaa ggttgtacgc agaatttcgt gtcgtcgcac gatttcattg
     9721 atacttcgct ccagaaccgc catgtcgaat ggcgaggtca gtcggagcgc gtacaggata
     9781 ttgaaaaaag gcaagcccgg cagcgcgtgc tgtaatttcc aaagccgttc ctgggcaaag
     9841 gaggccggca gtccatcttc tcgaactatg ggcacaatgg ccgaagaaag tggccgcgcc
     9901 tggtcggttt ggatcaacgt ctcgagatgg ccagccattt cggcaaccgt cggcgcctca
     9961 aaaaagcggg agacctcgac cttgagatgc atgctttggt gcatgtaggt gagaacgcgc
    10021 gaggccagga gagaatctcc gcccgatgcg aagaagtcat catggatccc gacccgttcg
    10081 acttggaggg tctctgccca gagtgcgact agctccttct ccagcggggt ccgtggcgcg
    10141 acgaaggttt gcggcatggc ttggctcgcg agccccaact tagccgccaa gccgacacgc
    10201 tgcactttgc ccgtcgagcc tttcgggagt tcgccgacga tgagcacttg gcgcgggact
    10261 ttgaagtcgg cgatgcgccc gacggtgaac tggcgaatgt ctttgggcgt tgccgcagct
    10321 tggggccgca acacgacagc ggacgcgacg tcctcaccga gcgtggcgtg gggaactgcg
    10381 aaggttacag cctccgccac cgccgggtgc tctagcagca cttcgtcgac ttcgcgtggg
    10441 gcgatttttt ccccgccgcg attaataatc tcctgggtgc gaccggcgag aaagaggtat
    10501 ccgtcggcat cgaaaaagcc gcggtcccct gtcttgaacc aatcacccgc aaatgcggcc
    10561 cgggtcgccg ttggatcccg gtcatagcct ggcatgacgc tcgcgccgcg gacgacgacc
    10621 tgccctatct gaccgtcgtg cagcaaagcc cccccttcgt ccataatgcc gacgtccaaa
    10681 cccaccggta gcccgaccga acccggctta cgctgccgcg gcggcagcgg attgcaggcg
    10741 accggtgagg aagcggtttc cgtcataccg taaaactcga tcaccgaggt ctcgaacgtc
    10801 cgctccaggt ccgcaaagac gcgaggtggc agaggtgccg acgccgagcg gaccaaacgt
    10861 agccgacagt ctgccgcttg ctcgcgagcg tggcgagcct gggcgagaat tgcttgatgc
    10921 atggtgggta ccgcggagta ccacgtcggc cgaaaggcgg ttagccaggc aaagaagctg
    10981 ttgacgtcac agccgggcgt gcacacgaca ctcgcgccgg ccgccagcga ggccagcacc
    11041 gtggcaatga gaccgtgccc atgaaacagg ggcagcacgt tcaagcaccg atcagtttcg
    11101 cccagcgcca ctgccgccgc cgaactataa gccgacgtgc aaatgttgac ctgcgtcagc
    11161 gggacaatct tgggctgcga cgttgtgccc gacgtgaata gcagcagcgc cacgtcgcca
    11221 ccgctgaccg attcgtgcga ccgcatgcgc caatggcgtc cctcgagcgt gaacacgcca
    11281 gcctctgcgt cgaacgtagc cgacaactcg ataacgcgaa tgccgcgtga gagcgccaca
    11341 cgacgcgccg gcgagtcgat tccagcctgc gtgattaatg cgcgaggccg cagatcagcg
    11401 aaatatcggt ctagttcctc ggctccatag gcagggttca tcggggcgca cgccgcactc
    11461 gacgccacgg tgagaatagc gaccgccatc tccggaccgt tcggcaacac gacggcgata
    11521 cggtcatgac ggcctatccc catggcccgc agcgcatgcc cgaccttgtc gacctgctga
    11581 taaagacggc cgtaggtcag cggagcgcgc cccagagcga ggatcgcggg cgcgccggga
    11641 atacgcttgg cctgacgttc cagcaggtga gggaggcagg aaaacggaac ggaaaatgtt
    11701 gatggtgcgg aacgct
//
LOCUS       DATLJT010000979         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4201288,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000979 DATLJT010000000
VERSION     DATLJT010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201288"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VM910_22575"
     CDS             <1..1086
                     /locus_tag="VM910_22575"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HVI65343.1"
                     /translation="FDYHEGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPNY
                     PDNSRFWNVIDKHKVNTFYTAPTAIRALMQGGDEPVKKTSRKSLRLLGSVGEPINPEA
                     WEWYHRVVGEDRCPIVDTWWQTETGGILITPLPGATRLKPGSATNPFFGVVPEIVDAD
                     GKLLEGDATGNLCIARSWPGQMRTVYGDHARFEQTYFSTYKGKYFTGDGCRRDADGYY
                     WITGRVDDVINVSGHRMGTAEVESALVAHALVSEAAVVGYPHDIKGQGIYAYVTLMEG
                     TEPSEELRKELVAWVRKEIGPIASPDQIQFAPGLPKTRSGKIMRRILRKIAEDEPGSL
                     GDTSTLADPAVVDDLVKNRQNRRQSAA"
     gene            1427..1942
                     /locus_tag="VM910_22580"
     CDS             1427..1942
                     /locus_tag="VM910_22580"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI65344.1"
                     /translation="MRKRIAMAFLATIGMVVLATDAAQARGELVGVPNGEYSPGTIVV
                     RTNERHLYLILDSGHAMRYPVGVGRHGKQWAGDTRIDGKYRNPAWSPPQEVRRDKPFM
                     PDVIPGGSPRNPMGVAAMTLAGGEYAIHGTNTPGSVGGFVSYGCIRMLNTDITDLFQR
                     VSVGTRVIVTH"
     gene            complement(1988..2242)
                     /locus_tag="VM910_22585"
     CDS             complement(1988..2242)
                     /locus_tag="VM910_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HVI65345.1"
                     /translation="MEKPGFKERAVPEHDDASHAATTAASPPRKPLTPAAQRALAEAE
                     ARRQAAAADAKPRPKEYQGPKGPEPTRYGDWENKGIASDF"
     gene            2379..>2708
                     /locus_tag="VM910_22590"
     CDS             2379..>2708
                     /locus_tag="VM910_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVI65346.1"
                     /translation="MPPRLARPSEVPGLAARRIAADVLDGVLHKHRTLDDQLDGAAAH
                     PGLKTLADRDRALMRKLVATILRRLGTLGHLLSRLLDRGIPTDAPRAQSALLIGAAQI
                     LWMDVPDH"
BASE COUNT          484 a          863 c          860 g          501 t
ORIGIN      
        1 ttcgactatc acgagggcga tatctactgg tgcaccgccg acgtcggctg ggtcaccggc
       61 cacagctaca tcgtctatgg gccgctcgcg aacggcgcca ccacgctgat gttcgaaggc
      121 gtgccgaact atccggacaa ttcgcgcttc tggaacgtca tcgacaagca caaggtcaat
      181 accttctaca ccgcgccgac cgcgatccgc gcgctgatgc agggaggcga cgagccggtg
      241 aagaagacct cgcgcaagag cctgcgcctg ctcgggtcgg tcggcgagcc gatcaatccg
      301 gaagcatggg aatggtatca ccgcgtggtc ggcgaagacc gctgtccgat cgtcgacacc
      361 tggtggcaga cggaaaccgg cggcattctg atcacgcccc tgcccggcgc cacccggctg
      421 aagccgggtt cggcaaccaa tccgttcttc ggcgtggtgc cggagatcgt cgatgccgac
      481 ggcaagctgc tggaaggcga cgccacgggc aacctctgca tcgcgcggtc atggcccggc
      541 cagatgcgca cggtttacgg cgatcatgcc cgcttcgaac aaacctactt ctcgacctac
      601 aagggcaaat atttcaccgg cgacggctgt cggcgcgacg ccgacggcta ttactggatc
      661 accggacgcg tcgacgacgt catcaacgtc tccggccacc gcatgggcac cgcggaggtc
      721 gagagcgcgc tggttgcgca tgcgctggtg tcggaagccg cggtggtcgg ctatccccac
      781 gacatcaagg gtcagggtat ttacgcctat gtgacgctga tggaggggac cgagccgagc
      841 gaagagctgc gcaaggaact ggtcgcctgg gtccgcaagg agatcggacc gatcgcctcg
      901 ccggaccaga tccagttcgc gccgggcctg ccgaagacgc gatcgggcaa gatcatgcgg
      961 cgcatcctgc gcaagattgc cgaggatgag cccggcagcc tgggcgatac gtcgaccctg
     1021 gccgatcccg ccgtggtcga cgatctcgtc aaaaaccggc agaaccggcg gcagagcgca
     1081 gcataaaccg aagcggcgcc gtttttcctg ccactcttcc cgcatgcaga tcgtatgcgg
     1141 gaagagcaag aacgcgcaac cgcgcgccca cgcggcattc gcgtcatcgc caaaggaatg
     1201 tcggagagct gtggcgcggc cgcggcggtt ttccggcgag tgttgttttc aagtcattta
     1261 ctttgtttgg aactttttct tttcttcgcc tcgtcaggcc agtccggcag cctgtgtaga
     1321 aaccatccgg ttaaagcacg cggttaaaaa tacgcgagca gaccgccttt gagagcggtt
     1381 tgaaccagtt gtgtcccttc aggggtgtgc gagcggagta ggattgatgc ggaagcggat
     1441 tgctatggca ttcctggcca ccatcgggat ggttgttctg gcgaccgatg cggcgcaggc
     1501 gcgcggcgaa ctcgtcggtg ttcccaacgg cgaatattcg ccgggaacga tcgtggtgag
     1561 gaccaacgag cgccatctct atctcattct cgattccggc cacgccatgc gttacccggt
     1621 cggcgtcggc aggcacggca agcagtgggc gggcgacacc cggatcgacg gcaaataccg
     1681 caacccggca tggtcgccgc cgcaggaagt caggcgcgac aagcccttca tgccggacgt
     1741 gattcccggt ggctcgccgc gcaatccgat gggcgtcgcg gcgatgaccc tggccggcgg
     1801 cgaatacgcg atccacggca cgaacacgcc gggttcggtc ggcggcttcg tctcctatgg
     1861 ctgcatccgc atgctgaaca ccgacatcac cgacctcttc cagcgcgtgt ctgtcggcac
     1921 cagggtgatc gtgacgcact gatcgcttct ctttcgagaa tgcaaaagtc gcgcctctcg
     1981 cgcggcttca gaaatccgag gcgatgccct tgttctccca atcgccatag cgcgtgggtt
     2041 ccggcccctt cggcccctga tattcttttg gccgcggctt ggcgtcggcg gcagccgcct
     2101 gccggcgcgc ttcggcttca gcgagcgcgc gctgggcggc cggcgtcagc ggcttgcgag
     2161 gcgggcttgc tgcagtcgtc gcggcgtgtg aagcatcgtc gtgttcaggc actgctcttt
     2221 ccttgaaacc gggcttctcc atcaacacat cgtgcatcgc agcgaaaaag ccagaggggt
     2281 tcttatattt tgcatattcg catggcagag ctgtggtctc gtcaatggca tgcgccggac
     2341 tctcgcggat ttgcctttcc cctgagtaat ggtccagcat gcctccaaga ttagctcgcc
     2401 cctccgaagt gcccggtctt gcggcgcggc gaatcgcggc ggacgtgctc gacggcgttc
     2461 tgcacaaaca tcgcacactt gacgatcagc tcgatggcgc agccgcgcat cccggcctga
     2521 agacgctggc cgatcgcgat cgcgcgctga tgcggaagct ggttgcgacg atcctgcgcc
     2581 ggctcggcac gctcggccat ttgctgtcgc ggctgctcga ccgcggcatc ccgaccgacg
     2641 cgccgcgcgc gcagagcgcg ctcctgatcg gtgccgcgca aattctctgg atggacgtgc
     2701 ccgatcat
//
LOCUS       DATLJT010000980         1538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1444523,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000980 DATLJT010000000
VERSION     DATLJT010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444523"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            988..>1538
                     /locus_tag="VM910_22595"
     CDS             988..>1538
                     /locus_tag="VM910_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI65347.1"
                     /translation="MRAQSRTSVLIIDDHPVVLQGLRHVLEDAGVKPVLEARDVTSGY
                     RLFRRNRPDVIIVDLGMQGSLLAGLDLIRRMRSHEPCTCILVFSMHSDPMIVARALEA
                     GATGYLLKDTSSDELMKAFDEVRSGKPYLSKDLAKRVAFLRPQVGLTPLVDLTSRESQ
                     MLSLLAKGKTYSSIADELGVSYKT"
BASE COUNT          331 a          458 c          454 g          295 t
ORIGIN      
        1 tgcggtcgat gccgagaccg gtgatgggcg tcggagcgac gaactgaaat ggagacggtg
       61 cactcacgac gggcgcgggc gccggctcag acggcgatgg cgccgctggc tccggccgct
      121 caggcggcga tggcgccgcg cggcggggtg caggctctgc gcgccgaggt ggccggtgcg
      181 caggccgcgg ggcgctgact gtgacctccg gtagcgtttc ggatacttgg ctccaggccg
      241 gcccaggcga ggtcagccat cccggcaacg gcaatgcgct caaggcgacg gcacccaaaa
      301 gccgtcgttt ccaaccagaa agcgaagatg accttcgacg atccccagcc gcccccaaca
      361 tgacgccccc caacgcaaaa ctcaaccgtg ccacgttaaa actcgttaat ggccccgtgt
      421 cttttctgcg gcaaagtgga tagggacctt tgtgcaatgc gtcccctatg gacaccgttc
      481 aagtccgggc tgcgtgtttc atgcgaggcc aggctcccct cgcagcaacc tcattcgggc
      541 tggagctaaa cagccacgat cgcatcaaga atcatttgca tgcgcagtga aaccgtgaac
      601 gcggctcttc cgccccgtgt gagccaaaag aaagctgctc ggcaagttca tgagggaaga
      661 ttaggcaaaa tctcttgcca atatagcccg gagcttttgc caagttagtc ggcagagcgt
      721 cgccaacctg aaatacgcat ttatatcata gtgacttttt ggccactatt ttcgcaacct
      781 ctaagtataa ggcaacagag gcaacagccg taatggccgg aatggctaag ccagccgatc
      841 taagctactg ctggccatac gctgccccgc cgaggcgagt ggtccacatc tagcgcctca
      901 aaggacattg agcgaatgct ctatctggct cttagcatct cataaatgta gaactcgcga
      961 acgaagcaac gtggatgata ttggagaatg cgcgcgcagt cgcggacgag cgttctgatc
     1021 atagatgacc atcctgtcgt gctgcaggga ttgcggcacg tgctggaaga cgccggcgtc
     1081 aagcccgtgc tggaggcacg cgacgtgacc tccggctacc ggcttttccg tcgcaaccgt
     1141 cctgacgtga tcatcgtcga tctcggcatg caaggcagcc tgcttgcagg cctggaccta
     1201 attcggcgca tgcgatcgca cgagccgtgc acttgcatcc tggtgttcag catgcatagc
     1261 gacccgatga tcgtcgcgcg cgcgcttgaa gcgggcgcta ccggctattt gcttaaggat
     1321 acgtcctcag acgagctgat gaaggcattc gacgaggtac gctcgggtaa gccctatctc
     1381 agcaaagatc tcgcgaagcg agtcgcgttc ttgcgccccc aggtggggtt gaccccgctc
     1441 gtcgatctaa cttcacgcga gtcacagatg ctctcgctat tggcgaaagg caaaacttat
     1501 agcagtatcg ccgatgagct cggcgtcagc tacaagac
//
LOCUS       DATLJT010000981         7184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3512547,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000981 DATLJT010000000
VERSION     DATLJT010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512547"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VM910_22600"
     CDS             complement(<1..1075)
                     /locus_tag="VM910_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65348.1"
                     /translation="MFNADDLIWIDFEVFGGALDLKAAGAFRYVAEASTHTIVLSYAI
                     GNAPALTWHADGAILDWNCAPNELRAAYERRKTFAAWNASFDAAVWNYATLGFPFLAP
                     ERVIDPMVQAGVSNLPTDLEHASRYLGGPGKQKDGKALIRMFSIEGANPRECPAEWKR
                     FLAYARQDVEAMRDVYRKTRPLPHEEWRQYWAFEHINRRGVAIDMPFVRNAATLATED
                     AIAIGRRLTELTGGAVTRVTQAKRIATWMHDMLADAAMREVLTVGVPDDDDADDADET
                     EFSLTRDRVARVLAMLDIKRANGGLGFDEAKAHEAAELRLYGAGASPKKFARLEAQQV
                     DGVLRGQYRFAGAGQTGRMTSRGA"
     gene            complement(1163..1657)
                     /locus_tag="VM910_22605"
     CDS             complement(1163..1657)
                     /locus_tag="VM910_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65349.1"
                     /translation="MWGYVCFGPNKNDSPIGEVLVSVGQLKPDITKLPDKGFPWTEQR
                     AVNMKCISGIDAGIEVIFKINTQGGIQAIDGLIDTVRDRLNGNQHEGKVAPIATLGKD
                     SYPHKEHGRIWIPVLTITDWMSLDGPAPKPEPEPEPEPTPPQKPGSEPTSAAAEQPRR
                     RRVA"
     gene            complement(2221..3222)
                     /locus_tag="VM910_22610"
     CDS             complement(2221..3222)
                     /locus_tag="VM910_22610"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI65350.1"
                     /translation="MNVELPREPAHSPFGGSVAARVLRCPASVGLVEKVPPHLRKVSA
                     YADRGTALHAAITCLLAEDTHALVESLAGKTFGSYTITSDDIETALRPAYAHVEVLLD
                     DASGAEYYLERRVIFPTIADTFGTCDLLIRIGRKIYVIDFKFGAGVRVLALCPDGDED
                     VLNSQLMFYAAAARHSLPEFFAGVDEIALTILQPMSIELDAEMVSSVAVTHAELDAFI
                     AAYHSACEEALSDAPRLESGSWCRFCPARPICPAHTKPLLDLAAFTAPAPWGAAHAAP
                     ATPPPAKEAYLRLLAEGLNLVDAVKDIGVTLRDQAKRALQDGDDVPGYALSAGRAER"
     gene            complement(3219..3701)
                     /locus_tag="VM910_22615"
     CDS             complement(3219..3701)
                     /locus_tag="VM910_22615"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HVI65351.1"
                     /translation="MTEAITITVNGEVVAKGRPRMTRRGFIYTPAATRKYEAHARLAA
                     QVAMGSRAPLQDPVRIEVSIELPVPTSWSNKRRLAALAGKIAPTARPDIDNYIKTALD
                     AINTIVVTDDAQVVEIRARKQFSDQPKLVATIIPLDAVASNLRATQREVITTVEEAAA
                     "
     gene            complement(3710..4009)
                     /locus_tag="VM910_22620"
     CDS             complement(3710..4009)
                     /locus_tag="VM910_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65352.1"
                     /translation="MLVGTAVLRYTATVSYYADGRIGELFLNNHKSNSTADTNARDAA
                     IAFSFAVQHGADIEDIRKALCRETDGRASGPLGAALDLITNAIEPGPVPSPEMTE"
     gene            complement(4608..5267)
                     /locus_tag="VM910_22625"
     CDS             complement(4608..5267)
                     /locus_tag="VM910_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65353.1"
                     /translation="MSNIASHEEINSVVVLDELARRVRKSRQAAQQAGAALLHHCLDA
                     GDALNEAQARVSIPWKRWLKDNCSIPLAGGGLQPLSVRSAFVYQQLANHREKIEAAIS
                     QVGELSQRAALRLISKPKQAGKKEPEEEEETSAPENLVPAGTNLALSALKITSDEQVT
                     AALRILTLDWFIRVMPPEWNSDIQKRLRGHILRQEQKRHPNTRLKNLRLVHSGDGLAK
                     H"
     gene            complement(5348..5704)
                     /locus_tag="VM910_22630"
     CDS             complement(5348..5704)
                     /locus_tag="VM910_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65354.1"
                     /translation="MADQTGPRGKLLRGARRIAQVIYPELYIADDKAAIRKLYVEQGR
                     YPVFQLGRGDTELLALEDRLLSFLESHSRAAEAKAELAVSEAVAPPPSKTALSPKAAS
                     VRKAAPPRTRRRRRAA"
     gene            5849..6406
                     /locus_tag="VM910_22635"
     CDS             5849..6406
                     /locus_tag="VM910_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65355.1"
                     /translation="MPPFTSPIFTYAEAESALGAALDFGLVHQRGTIKLRLKHLQRLN
                     LVELNLRKKQRAKYSRAQIVLWLLALILAETGADPVQVVAALKRSWKHLASTIELATS
                     DEARSGRPYYMITWLRGLSGPVRQKPTLSIDLVQLQFPNPYAVPSEQLRQLREVVIGN
                     SNEMVALVNVTRPLSRLENALPSKS"
     gene            6409..>7184
                     /locus_tag="VM910_22640"
     CDS             6409..>7184
                     /locus_tag="VM910_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65356.1"
                     /translation="MSIRRRTWKTQSGESRSSWLVDYRDRDGQRHTASFERRKDADAY
                     HAQVAVDVRAGTHTAPSRSPTVTEAITDWLERGETEQLERATLKQYRELGAHIEQHLG
                     PTRLALLTTPGVNAFRDTLCRTMSRSMARKILTAFKGVLSEAQRRGNVAQNVAIGVSI
                     GPDKRKAGLEVGRDIPSPAEIQRILAAAPERARPLLVTATLTGVRSPARRAPRPPAGR
                     SLWRARPAKVAGGATHHSARPHGGEHAEGVAAGHYLHRPGV"
BASE COUNT         1317 a         2212 c         2239 g         1416 t
ORIGIN      
        1 gcgcccctcg actggtcatg cgccccgtct gcccggcgcc ggcaaagcgg tactgacctc
       61 gcagcacgcc gtcgacttgc tgcgcttcaa ggcgcgcgaa cttcttcggc gaggctcctg
      121 cgccgtacaa gcgtagttcg gcagcctcgt gtgccttcgc ctcatcgaag ccaaggccgc
      181 cgttggcgcg cttgatgtcg agcatggcga gcacgcgggc gacgcggtcg cgcgtcaggc
      241 tgaattctgt ctcgtccgca tcatcggcgt catcgtcatc aggaacgccg acggtcagga
      301 cctcgcgcat tgccgcgtcg gcgagcatgt cgtgcatcca ggtcgcgatc cgtttcgcct
      361 gcgtaactcg ggtgacagcc ccgccggtga gctcggtcag ccggcggccg atggcgatgg
      421 catcttcggt cgcaagcgtt gcggcgttgc gcacgaaggg catatcgatc gctacgcccc
      481 tccggttgat atgctcgaac gcccaatatt ggcgccactc ctcatgcggc agcgggcgcg
      541 ttttgcgata cacatcgcgc atcgcctcga catcctgacg cgcataagcg agaaagcgct
      601 tccactccgc cggacattcg cgtgggttcg cgccttcgat gctgaacatt cggatcagcg
      661 ctttgccatc tttctgtttg ccggggccgc cgagatagcg cgatgcgtgt tcgagatcgg
      721 tcggcaggtt ggaaacgccg gcttggacca tcggatcgat gacgcgttcc ggcgcgagga
      781 aggggaatcc aagcgtggcg taattccaga cggcagcgtc gaagcttgcg ttccatgcgg
      841 caaaggtttt gcggcgctcg taggcagcac gcagctcgtt cggcgcacag ttccaatcga
      901 ggatcgcgcc atcggcgtgc caggtcagcg ccggcgcatt accgatcgca taggatagaa
      961 cgatggtgtg tgttgatgct tcggcaacgt agcggaacgc gccagcggcc tttaggtcga
     1021 gtgcgccgcc aaacacttca aaatcaatcc aaatgagatc gtcggcatta aacatcgcga
     1081 tctcctaggt tttagagaag tcaggcgagg ccgccgcgcg gccagcctcg ccttgatcag
     1141 gtgcgcgcgt acgcgcaccg ccttacgcga cgcgacggcg ccgcggttgc tcggcagccg
     1201 cagatgtcgg ctccgatcct ggtttctgcg gcggtgtcgg ttccggttcc ggctctggct
     1261 ccggtttcgg cgccggacca tccagcgaca tccaatccgt gattgtcagc acgggaatcc
     1321 aaattctccc gtgctctttg tgcggatagg agtctttgcc gagagtcgcg ataggtgcga
     1381 ctttgccctc gtgctggttg ccgttgagcc ggtcgcgcac cgtgtcgatc aatccatcga
     1441 tggcttgaat gccaccttga gtattgatct tgaagatgac ttcgatgcca gcgtcgatac
     1501 cgctgatgca cttcatatta accgcacgtt gctccgtcca agggaagccc ttgtcaggaa
     1561 gcttggtgat gtcgggcttc agctgtccga ccgacacgag aacttctcct atgggagagt
     1621 cgttcttgtt cgggccgaaa cagacataac cccacatgaa ggttcgcggg tcgaaggccc
     1681 acaggctgcc gtcttcgacg acagtgcggc ttctaccgaa catccaagct ccgttctcac
     1741 gggacttgaa ttgcatcagc ggtcgcccag agcggccggt cgatgtcgat gtagcgacgc
     1801 tgttgagcat cgattgcagc gccgctaggg aggtaagtgc acctcccgct ggcccggggg
     1861 cgacttgttt gctcccgggc tgttcgtggc cgccgttatc attggcggga tcgtgatcgc
     1921 gattgcgatc atgatcgtgg tcgtcgctca tgattgcctc ctttggaaag ccgtgagcgc
     1981 ctcggagaac aagcgcatac gctcaacccg tccgagtacc gggtcgtgcg cgttctcact
     2041 ccgcaccagt gagacaccgg agcggcgtga aacgattaat tcctgtggga ctttgaggcc
     2101 gcgcgccttc gcccgaatct cgagctgctt gggcgaacgc atcatctcgg cttcgataat
     2161 atcatcgtgg tcgaaaccga ggttcagtaa ggctacctgc gcgacgattt catcgcgcca
     2221 ctagcgctcg gcgcggccgg ccgagagcgc gtagccaggc acatcgtcgc cgtcttgcag
     2281 cgcgcgcttg gcttgatcac gtagcgttac gccgatgtct ttgaccgcat cgacgagatt
     2341 cagcccctca gcgagaagcc gcaggtacgc ctctttcgcc ggcggcggtg tggcgggagc
     2401 cgcatgggct gcaccccacg gcgccggcgc cgtaaacgcg gcgagatcaa gcagcggctt
     2461 cgtgtgcgcc gggcagatcg gtctcgccgg acaaaaccgg caccatgatc ctgattccag
     2521 gcgcggtgca tcggagagcg cctcttcgca ggcgctgtga taggccgcga tgaacgcgtc
     2581 cagctcggca tgcgtcaccg cgaccgatga gaccatttcg gcatcgagct cgattgacat
     2641 cggctgcagg attgtcagtg cgatctcgtc gacgccggcg aaaaattccg gcaacgaatg
     2701 gcgggcggcc gcggcgtaaa acataagctg cgaattgaga acgtcctcgt cgccgtcagg
     2761 acagagcgcg agaacgcgca cgccggcacc aaatttgaaa tcgatcacat agatcttgcg
     2821 gccgatgcgg atgagcagat cgcaggtgcc aaaggtgtcg gcgatagtgg gaaagatgac
     2881 gcgccgttca agatagtatt cagctccgga cgcatcatcc aggagcacct ctacatgggc
     2941 gtatgccggc cgcagggcgg tctcgatgtc gtcggacgtg atggtgtagc tgccgaacgt
     3001 tttgccggcg aggctctcga cgagggcatg cgtgtcttcg gcgaggaggc aggttatcgc
     3061 cgcatgaagc gccgtgccgc ggtcggcata ggccgaaact ttccgcagat gcggagggac
     3121 tttttcgacg aggccgacgg acgctgggca gcgcaaaacg cgcgccgcga cgctgccgcc
     3181 gaagggcgaa tgagccggct cgcgcgggag ctcgacgttc atgctgcagc ctcttccacc
     3241 gtggtgataa cttcgcgctg cgtggcccgt aaattcgagg caactgcatc gagcgggatg
     3301 atggtcgcaa ctagcttcgg ttgatcgctg aattgcttgc gggctcgtat ctctacgact
     3361 tgggcatcgt cggtgacgac gatagtgttg atcgcgtcga gcgcggtctt gatgtagttg
     3421 tcgatatccg gccgagcggt tggcgcgatt ttccccgcaa gcgcggcgag ccggcgtttg
     3481 ttgctccagc tcgtcggcac cggcagctcg atcgatacct cgatcctgac cggatcctgc
     3541 aagggcgctc ggctacccat cgcgacctgc gcagcgagcc gggcatgggc ctcgtatttg
     3601 cgggtggcag caggtgtgta gatgaagccg cgccgcgtca tccgcggtcg gcccttcgcg
     3661 actacttcgc cgtttacagt aatggtgatt gcctcggtca tggctgccac tactccgtca
     3721 tctcgggtga cggcaccggg ccaggctcga tcgcatttgt gatcaggtcg agtgcggcgc
     3781 cgagcggtcc tgaagctcgt ccgtccgttt cgcgacagag cgctttgcgg atgtcttcga
     3841 tgtcggcgcc gtgctgtacg gcaaacgaga acgcgatagc ggcgtcgcgg gcattggtgt
     3901 cggccgtgct gttggatttg tgattgttca ggaacagctc gccgatacgg ccgtcggcgt
     3961 agtagctgac tgttgccgtg tagcgcagca cggcggtgcc gacgagcata tcaaaatcaa
     4021 atgtttcgga ttgtctgcgg tcagaaaggc gctcgcgcag gcggcgacct tctcgtttgc
     4081 tgcgaggaat tcaagcacgc cgctgacacc aatcttctcg acgacggcgg cgagcaactt
     4141 ttcgactccc atcatcaccg gcggcactgc cgccggcacg atggctggca ccagcagagc
     4201 tgccgaggtg cgcgggagca ccagcggacg catcccacac ctcccttcct gccgctgttc
     4261 aggcgcaaag cagtgagcgc gagcggcgag accgaagttt aagccccacg ctacggcagc
     4321 caccctgatc gtgggatgac tcaagaggac cagaccgtcg acggcgtcgg ccgcgtcgca
     4381 agctttcacc ggtttcgatg cctgcaagtg aagcaggctg tggcaatcga caacgcgcga
     4441 cgccgccgcg gttgtcccgg tagtttctat gctgtaaagc atagcgttct tcctttcgta
     4501 ctggcctacc agcgagctcg acgttctcag cgtcgagctc gtcgccgttc cgcaaccagg
     4561 ccgatgccgc ggcggcttcc gggcgggatg cccggaaatc tcgatggtca gtgcttggcg
     4621 aggccgtcac cggaatgcac gagccgtaga ttctttaagc gggtgttggg atgccgcttc
     4681 tgttcttggc gcagaatgtg cccgcgcaac cgcttttgaa tgtcggaatt ccactcaggc
     4741 ggcatcacgc ggatgaacca atcaagcgtt aaaatgcgga gcgccgcggt tacttgttcg
     4801 tcgctcgtga tcttcagtgc cgatagggcc aggttcgtcc ctgccggcac caggttttcg
     4861 ggggcgctcg tctcttcctc ttcttccggc tccttcttgc ccgcctgctt aggcttcgag
     4921 atgagccgaa gtgcagcgcg ctggctcaac tcgccgacct gactgattgc agcctcaatc
     4981 ttttcacggt gattggcgag ctgctgatag acgaaagcgg agcgaacact gaggggctgg
     5041 agcccgcctc ccgccaatgg gatcgagcaa ttatctttga gccaccgctt ccagggtatg
     5101 gacactcgcg cttgggcttc attcagcgca tcgccggcgt cgagacagtg gtgcagcagt
     5161 gctgcacccg cctgctgggc ggcctgccgg gactttcgga cccgccgggc gagctcatcc
     5221 aggacaacaa cggagttgat ttcttcatgg gaggcgatat ttgacatgac gtcgatcctc
     5281 gaattgacaa ccgggatcac agaaactcct cgccggacga accactcagg cggggagttt
     5341 cactttttta ggcagcgcga cggcgccggc gggtcctggg cggggccgcc ttccgaacgg
     5401 aagcagcttt cggggacagg gcggttttcg acgggggagg agcgacggcc tccgacaccg
     5461 ccaattcggc cttagcctcg gccgctcggg aatgggattc aagaaacgaa agaagccggt
     5521 cttctaaggc cagcagctcg gtgtcgccgc ggcccagttg aaagactggg taccgaccct
     5581 gttcgacata gagtttccgg atcgccgcct tgtcgtcggc gatgtaaagt tctgggtaaa
     5641 tcacctgcgc gatccggcga gcgccacgta atagtttgcc gcgcggacct gtttggtcgg
     5701 ccatggggaa gctccaggtc atttaggata agcttcatcc aaacacagga ttgaataggt
     5761 tgcaatagtc ttgctagaga atttgccttg cggttcctac caatctgccg atagaagaca
     5821 aaccggtact cttttgaaca ggtttgtcat gccgcccttc acatcaccga ttttcactta
     5881 cgccgaagcg gaatccgctt tgggtgccgc gctcgacttc ggactggtgc atcagcgcgg
     5941 aacaataaag ctgaggctca aacaccttca gcgcctcaat ctggtcgagc tcaacctcag
     6001 gaaaaagcaa cgggccaaat acagccgtgc ccagattgtt ctgtggctac tcgcgctgat
     6061 cctggcagag acaggcgcgg atcctgtaca ggtcgtggcc gcgttgaaac gaagctggaa
     6121 acatctcgcc agcacgatcg agctcgcgac cagcgatgaa gcacgtagcg ggcggcccta
     6181 ttatatgatc acgtggttgc gcggcttatc cgggccagtc agacaaaaac ctaccctctc
     6241 gattgacctc gttcagcttc agttccctaa tccatacgct gtaccaagcg aacagctgcg
     6301 tcagctgcgt gaggtggtaa ttggcaattc gaatgagatg gtcgcgcttg ttaatgtgac
     6361 ccgccccctt tcccgactcg aaaacgctct cccctccaag tcctgaaaat gagcatccgc
     6421 cgccgcacct ggaaaaccca gagcggggaa tcccgctcgt cgtggctcgt cgactatcgc
     6481 gaccgtgacg gccagcggca caccgcctcc tttgaacgca ggaaggacgc cgacgcctac
     6541 cacgcccaag tcgcggtcga tgtccgcgcc ggcacccata ccgcccccag ccgcagcccg
     6601 accgtcaccg aggccatcac cgattggctg gagcggggag agaccgagca attggagcgg
     6661 gccaccctga agcaataccg ggagctcggc gcccatattg agcagcatct tggcccgact
     6721 cggcttgccc tcctgacgac cccaggtgtg aacgcgttcc gggacaccct gtgcaggacg
     6781 atgtcgcggt cgatggcgcg caagatcctt accgcgttca agggcgtcct cagcgaggcc
     6841 cagcgccgcg gcaacgtcgc ccagaacgtc gcgatcgggg tgagcatcgg ccccgacaag
     6901 cgcaaggccg gcctcgaggt ggggcgcgac atccccagcc ccgccgagat ccagcgcatt
     6961 ctcgcagccg ccccggagcg cgcacgcccg ctgctagtga ccgcgaccct gaccggggtg
     7021 cgctctccag cacggcgagc tccacgtccg ccagcgggcc gatcgctatg gcgtgctcgg
     7081 ccagccaaag tcgcgggcgg ggcaacgcac cattccgctc ggccccatgg tggtgaacac
     7141 gctgaaggcg tggcggctgg ccactacctc caccggcctg gtgt
//
LOCUS       DATLJT010000982         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_296634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000982 DATLJT010000000
VERSION     DATLJT010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VM910_22645"
     CDS             complement(<1..182)
                     /locus_tag="VM910_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65357.1"
                     /translation="MVFVGLWDEWDMVSILEGHGFIGDLEVEHIQDLLQAGRDPEIVL
                     KKHVREAYLKYYKTTR"
     gene            complement(335..1006)
                     /locus_tag="VM910_22650"
     CDS             complement(335..1006)
                     /locus_tag="VM910_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65358.1"
                     /translation="MTYIREFDRATMIEIAPHCYLNETAARKQFSDSTKHSDISAEST
                     NSVDCSSLIPLRDRLVGPDVPASSPLHRRSRSMLFTSCLVGVILAGGTSAVIALRQFS
                     LADIWRGRAQLHQPTLTMRGEQRLIARSSRGSSGEPIPLGLTIYGPAEGAVVIITGLL
                     PGMELSNGIEVDVDRWEVPATEVSYAWIAPPDGFVGSALLTAELRSTDDSIADRQAIQ
                     LEWVP"
     gene            1261..1596
                     /locus_tag="VM910_22655"
     CDS             1261..1596
                     /locus_tag="VM910_22655"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HVI65359.1"
                     /translation="MLPGCAAPTSPNAALSNQTTNLYSDLLVHHMGKGLADGITQGGA
                     GPDEFRTAPLWGVGQRIFFLHDGRTTNLVEAIRDHQSDGSEANEVIKRFNRLTTQEQQ
                     DVIDFLRSL"
     gene            complement(1571..2617)
                     /locus_tag="VM910_22660"
     CDS             complement(1571..2617)
                     /locus_tag="VM910_22660"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65360.1"
                     /translation="MKRREFITLLGGAAAWPLAARAQQPAMPVVGFLHPSSPDPFRPR
                     AFRQGLKDAGFIEGENVAIEYRSADDQIDRLPALATELVQRRVAVIATGSPPAVFAAK
                     AATTTIPIVFVVGEDPVRLGLVTSLARPGGNLTGINIFTSELAAKRLELLREVVPGAT
                     RIAVLVNPANATQTASALRGVEPAARAMGLQIQVLNASTNREIDAVFATLASERPDAL
                     FVSNDAFFVDRRVQLALLAVLHRVPAIYQDRLYAEVGRPDQLRSQYCGCISPCRRLHR
                     PHPQGGKACGFAGRAVEQVRVRHQRLDRQDAWTHRAGQAARCRRRGDRMIGRREFTAP
                     NAIRFFVKATARNQ"
     gene            complement(2617..3027)
                     /locus_tag="VM910_22665"
     CDS             complement(2617..3027)
                     /locus_tag="VM910_22665"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65361.1"
                     /translation="MATPPSSVMNWRRLRSGMGTRCASLPQAQDAPEAPAGLTIVCVE
                     IISLAARYRLPAVYPARFFAELGGLLSYGNDVNDNFRRAATYADRILKGEKPNELPVQ
                     APVKFQLVINLKTAKALGLDVPWFLQQSADEVIE"
BASE COUNT          719 a         1015 c         1003 g          679 t
ORIGIN      
        1 aatctggttg tcttgtaata tttcaaatac gcttcgcgca cgtgcttctt caggacgatt
       61 tccggatcgc gaccggcttg taacaaatcc tgaatatgtt cgacttctaa atcaccgatg
      121 aagccgtgac cttcaagaat actgaccatg tcccattcgt cccatagtcc gacgaacacc
      181 atgagcaagt caaaatgtag ctcccttccc agaggtgtat ttcgccacgt gtcgcttgtg
      241 cccggaaccc gctaatttcg gatctgctca tggtcgtgaa acctccagcg taccgcgcac
      301 gattgtattg attctttgca aaccccggtg ggctctatgg cacccattcc aactgtatgg
      361 cttgccgatc ggcgatgcta tcatcggtcg agcgtagttc ggcagtgaga agagcggaac
      421 cgacaaaacc gtccggcggc gcgatccaag cgtaactaac ctccgttgca gggacctccc
      481 atctgtcgac gtcaacctca atgccgtttg acaactccat tccaggcagc aggcccgtga
      541 taatgacgac cgcgccctcg gccggtccgt agatcgtcag cccgagtggt ataggttctc
      601 ccgaacttcc gcgcgacgat cgggcaatta gccgctgctc gcctcgcatc gttaatgtcg
      661 gctgatgcag ctgcgccctg ccgcgccaaa tatcggcaag cgaaaactgc cgcaaggcta
      721 tgaccgcgct ggtgccgccg gccaaaatca ccccgactag acagctcgta aagagcatag
      781 atctgcttcg gcggtgcaac ggtgatgatg caggaacgtc aggaccaacc aaccggtcgc
      841 gcaaagggat cagcgagctg caatcgaccg aattggttga ttcggccgaa atatcagagt
      901 gtttggtgct gtcgctaaat tgcttccgtg ccgctgtctc attgagatag caatgcggag
      961 cgatctctat cattgtggcg cggtcgaatt cgcggatgta cgtcactgtt acctcccgat
     1021 cgaggggagc cgcaacccga cccgttctgc acgacccgcg gtgtcagtcc gatttagtgt
     1081 caggccgatt gacatctccc tgctggagcg ggacaagagg cgagagttca aacctctgcg
     1141 catcgctgac tttgtcaagc gcagttttgc ggtttgcgcg atagtcgacg catgggcacg
     1201 gggttaatac tggccagggg agctaacctt aattgcagag ctaacgttaa ttaacgttgg
     1261 atactccctg ggtgcgcagc accgacaagc ccgaacgctg cgctttccaa tcagacgacg
     1321 aatctttact ctgaccttct cgtccaccac atgggcaaag gcctcgccga cggcatcaca
     1381 caaggtggtg ccggccccga cgagttccga accgctcctc tttggggtgt cggacaacgt
     1441 atcttcttcc tccatgatgg acggacgacc aacctggtgg aagccatccg agatcaccag
     1501 agcgatggca gcgaggcaaa cgaggtcatc aaacgtttca ataggctgac tactcaggag
     1561 cagcaggacg tcattgattt cttgcggtcg ctttaacgaa gaatctgatc gcattaggag
     1621 cggtgaactc gcgccgcccg atcattcgat cacctcgtcg gcggcagcga gcagcttgtc
     1681 cggcacggtg agtccaagca tcctggcggt cgaggcgttg atgacgaact cgaacttgct
     1741 cgactgcacg accggcaaat ccgcaggctt tgcccccttg aggatgcggc cggtgtagac
     1801 gccgacatgg cgaaatgcat ccgcaatatt ggctccgtag ctgatcaggc cgtccgactt
     1861 cggcgtatag gcggtcttga tatattgcgg ggaccctgtg gagtacagcc aacagggcca
     1921 attggacgcg ccggtcgaca aagaaggcgt cgttgctaac gaagagggcg tcgggtcgct
     1981 cgctcgcaag cgtcgcgaac acggcatcga tctcgcggtt ggtgctggca ttgagaacct
     2041 ggatttgcag ccccatggcc cgagcagccg gttccacgcc tctcaatgca gacgcggtct
     2101 gcgtagcatt ggccggattg acgagcacgg caatgcgagt ggccccgggc accacctcac
     2161 gcaacagttc cagccgcttc gctgccaact cgctcgtgaa aatgttgatc cccgtgaggt
     2221 tgccgcccgg ccgggcgaga ctggtgacaa ggccaagcct gaccgggtct tcgccgacca
     2281 cgaagacgat ggggatcgtc gtggttgccg ccttggccgc gaataccgca ggcggactgc
     2341 ccgtcgcgat caccgcaacc cgtcgctgca ctaattcggt cgccagcgcc ggcagccgat
     2401 cgatttgatc gtcggccgag cggtattcga tggccacgtt ctcgccctcg atgaaacccg
     2461 cgtccttcag gccttggcga aatgcacgcg ggcggaaagg atccggcgat gaggggtgga
     2521 ggaacccgac caccggcatc gccggctgct gcgcgcgcgc cgcgagcggc cacgctgccg
     2581 cgccgccgag cagcgtgatg aactcgcgcc ttttcatcac tcgatcacct cgtcggcgct
     2641 ttgctggaga aaccaaggca cgtccaagcc gagcgccttg gcagtcttga ggttgatgac
     2701 cagctggaac ttaaccgggg cctggacagg aagctcgttt ggcttttcgc ccttcagtat
     2761 gcgatcggca taagtcgctg cacgccgaaa attatcgttc acgtcatttc cataggacag
     2821 caggccgccg agttcagcga agaagcgggc cggatagacg gcagggagac gatagcgagc
     2881 cgccagcgat atgatctcaa cgcacactat agtgagacct gcgggcgctt ccggggcatc
     2941 ctgagcctgc ggtaagctgg cacagcgggt tcccatgccc gacctaagac gacgccagtt
     3001 catcacgctg ctcggcggcg tggccactcg cggcgcgcgc ccagcagcgg tccgcaggac
     3061 agccctcgcg agagagggcc agttgagaga gattcgcttt gaacgcagaa cgattggacg
     3121 tggctggcca gcgttcggtc aattggcagt gacgaattag tctgcaggtc aatcaagacg
     3181 acgtgtcaag ccaagagcct cattgaatac tcggacggtc aataggtggc cgacgtggac
     3241 gtcgcggccg ccgaggaata aagcaagaag agcccggcat caaccgggcg tcgtggccaa
     3301 gcaatgtgag cccccttctt gtgtcggctt gcgcgctgcc gatttggtat cgcagtgatc
     3361 ggactcgaca tgaactttgg tagctgaagc catcggcccc atgtacggcg acatcg
//
LOCUS       DATLJT010000983         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2594556,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000983 DATLJT010000000
VERSION     DATLJT010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594556"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VM910_22670"
     CDS             complement(<1..556)
                     /locus_tag="VM910_22670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI65362.1"
                     /translation="MRLLRDGLVLSLSRQPSLAVADSTDLTISPTHIAAARPDVVLLD
                     VGTPGGLAALLALRQALPDLKIVAIAVSDIEQEVVACAEAGVSGFVSRDGSIQDLVTA
                     VDCAMRNELVCSPRIAGVLFRRVAAIGSERSHGPDNGALTRREHEIVSLMTQGLSNKE
                     IARQLRIQNATVKNHIHSILAKLHV"
     gene            complement(1022..1306)
                     /locus_tag="VM910_22675"
     CDS             complement(1022..1306)
                     /locus_tag="VM910_22675"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HVI65363.1"
                     /translation="MKDLNLLKPMQAQIEFGSGERMALGGFSVVDRARLKTLSGNMLA
                     QLVQSDELELSYAHLVSIYAHLVLMRNFATVRDRLAEAQPASAGGAPLIH"
     gene            complement(1303..1764)
                     /locus_tag="VM910_22680"
                     /pseudo
     CDS             complement(1303..1764)
                     /locus_tag="VM910_22680"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
     gene            complement(2022..>2235)
                     /locus_tag="VM910_22685"
     CDS             complement(2022..>2235)
                     /locus_tag="VM910_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HVI65364.1"
                     /translation="LDGEFFVTDDHCTHGPGSLSEGFVEGDVVECNFHGGQFNIRTGA
                     VVGPPCMVPIKTYPTVVEGGKVFIEV"
BASE COUNT          468 a          670 c          642 g          455 t
ORIGIN      
        1 gcacatgcag cttggcgagg atgctgtgaa tatgattctt gaccgttgcg ttctggatgc
       61 gcagctggcg cgcgatctcc ttgttggaaa ggccttgcgt catcagcgag acgatctcgt
      121 gctcgcgccg cgtcagcgcg ccgttgtctg ggccatggct tcgttcggag ccgatggcgg
      181 cgacgcgcct aaacagcaca ccggcgatgc gcggcgaaca aacgagctcg ttgcgcatgg
      241 cgcagtctac cgccgtcacc aaatcttgaa tcgacccgtc gcgggagacg aatcctgata
      301 ccccggcttc ggcacaggcg acgacctcct gctcgatgtc ggagaccgca atggcgacga
      361 tcttcaggtc gggcagtgcc tggcgaaggg cgagcagtgc cgccagaccg cctggcgtcc
      421 cgacgtcgag cagcaccaca tcgggacggg ctgcggcgat atgcgtcggc gatattgtca
      481 ggtccgtcga gtccgcgacg gccagcgagg gctgccgtga aagcgacagg acgagcccgt
      541 cgcgtaacag gcgcatgtcg ctgacaatga agaccgtggg tcccggatgc tggaggtttg
      601 atcttggtag agtttgcggc tccgacccaa caattgtatc cgcgcccggc gtttccacga
      661 cccaacctca tggaactcgt gaacttgaac agctcgaatc gaggcagacg tgtgaagttc
      721 ttaatgatca cactgccgcc tcaacgaggg gttgaattat gccataattt tcccggtatc
      781 cgcaagaaga gtccgttatg taacagtatc aggtaaccgt atcataacgg ttcgttaccg
      841 ttttgtttta ggttcgggga ttagctcttt ttgacacaag catgctcccc gccttcacat
      901 ttttgcatga ttgattgcgc atttttcatg agcatttcac gagctgaaag gttcggacct
      961 tcggccaccc tgcgattggt atgccagcac ctgagcagtt ccagggctgg ctggccgcgc
     1021 actagtggat tagcggcgcg ccgcccgcgc tagcaggctg cgcctcggcg agccgatctc
     1081 tcaccgttgc gaagtttcgc atcaacacga ggtgagcata gatcgacacg aggtgagcat
     1141 aggacagttc gagttcgtcc gactgcacca actgcgcgag catattgccc gataaggtct
     1201 tgagccgcgc acggtccacg accgagaacc ccccgagcgc catgcgctcc ccggaaccga
     1261 actcgatctg cgcctgcatc ggtttgagca ggttcagatc cttcacttct tgcaaaatgt
     1321 tagggtgcgc tggaattcga gttggtatcg ccccagcaat ttgagcagat tctccacgta
     1381 gggggtcggc ttaccatcat cgccaaacag tcgcttaccg cggccgctct ggttgaatcc
     1441 tgggaaggcc tcgtcaatgc aaagcgggaa cctcttgccg tcgtcgcggc tgaaaaagac
     1501 gaagggatag cggcggacga acgccggtat gtacttagcc taccagccgc cttccttggt
     1561 gacgtaaaga ttctcatcgg cacgaacgcc caacacggtg accggcgtca ccgcgtcgcc
     1621 agtgccgccg aacacgacgg cgtattccaa agccgcgcca gcaaactccg cggccatgag
     1681 cggcaccgag ttgatgtttc ggcagaatgc ataattaatc cacacttcaa gagaccagtt
     1741 gcgccgtgcc gagcgtgggc gcacgccagt tcgcttgctc gcaagcgcgg acggctgagc
     1801 actccacgct cggcattgta caatgctgtg atgctggtcg cggccgacga caacggccgg
     1861 cagcagccga ccccgaagat ctcgctctgt atttcttcgc cgaaaaattt gttaattata
     1921 atacttcttt tgttgataat aacgcttctg acacgcgatg acgcacacag aggcgacacg
     1981 tcggcgcgtc gccgccacga agccatttgc aaaaacgttg ctcacacctc gatgaagacc
     2041 ttcccgcctt cgacgaccgt gggataagtc ttgatcggaa ccatgcacgg cggcccgacg
     2101 accgcgccgg tcctgatatt gaactgcccg ccgtgaaaat tgcattcgac cacgtcaccc
     2161 tcgacgaagc cttccgacaa cgagcccggg ccgtgcgtgc agtgatcgtc agtcacgaag
     2221 aactcaccgt cgagg
//
LOCUS       DATLJT010000984         4564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2363048,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000984 DATLJT010000000
VERSION     DATLJT010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363048"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..139
                     /locus_tag="VM910_22690"
     tRNA            62..139
                     /locus_tag="VM910_22690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97..99,aa:Pro,seq:ggg)
     gene            complement(165..707)
                     /locus_tag="VM910_22695"
     CDS             complement(165..707)
                     /locus_tag="VM910_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HVI65365.1"
                     /translation="MVHPFFTIGHSTRPVSEFVDLLEAAQVGCVVDVRTVPRSRTNPQ
                     YNCDILPETLAQFQIGYEHIAALGGLRARSQDVPPDVNAFWQNQSFHNYADYTLTQPF
                     HGGLARLHALGHERRCAIMCAEAVWWRCHRRIIADYLIAAGETVFHILGKDRIDLARM
                     TDAAQPRPDGSLTYAAAPDR"
     gene            complement(725..940)
                     /locus_tag="VM910_22700"
     CDS             complement(725..940)
                     /locus_tag="VM910_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HVI65366.1"
                     /translation="MKKRFKLHDRVSWNSEAGRVSGRIVKVHTKDVNYKGYVHHASKD
                     EPQYEIKSDKTDHVALHKGAALRLKRR"
     gene            1280..1567
                     /locus_tag="VM910_22705"
     CDS             1280..1567
                     /locus_tag="VM910_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65367.1"
                     /translation="MTDTAIIERDVVVPAASEASRTPFSWSAAIAGALAAAGVTFIIV
                     ALGSGIGLSFASPYGSGPSATSLTIAAAVWLVMAQTRLRAGLSRLKPRSSD"
     gene            1679..1852
                     /locus_tag="VM910_22710"
     CDS             1679..1852
                     /locus_tag="VM910_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65368.1"
                     /translation="MQVHCGLFNNSPMDPLWLGVTAAGLLAFSIYEVAEAAFGRITSP
                     SLRQTAAKRGLAG"
     gene            1886..2668
                     /locus_tag="VM910_22715"
     CDS             1886..2668
                     /locus_tag="VM910_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HVI65369.1"
                     /translation="MSEDDLAALRRAVASLEHPGLAARLTNMVGKPIELIGYALPASA
                     SQAITAATSKALEMALEVALRSIHQAPRAGSQLFHKALATASGAAGGAFGLAALPIEL
                     PLSTIIMLRSIAEIARSEGEDLSDPEAALSCVQVFALGGRPGSTDASESGYFAVRGML
                     AKSVTQAARFIVERGVIEEGGPILVRFITQVASRFGVVVTQKLAAQAIPLVGALGGAA
                     VNYAFIEHFQEVARGHFTVRRLERAYGKEAVQKAYERFAQNG"
     gene            complement(2802..4505)
                     /locus_tag="VM910_22720"
     CDS             complement(2802..4505)
                     /locus_tag="VM910_22720"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65370.1"
                     /translation="MIRRLRLASGLVMLAYVTTHFVNHSLGLVSVQVMDFALEHIYQY
                     WASSLGSVLLYGAFATHYSLALWALWLRRSLKMPLAEATQLVLGFSIPFFLTDHVLQT
                     RVADTFYGANVGYYSHVLHTYFVANPLRGGLQFAVLVVAWVHAMIGLWFWLRLKPWYP
                     RWQPILYAFALLMPTLAILGALEGGRQVMALAQDPAWLAKQAQDHAPAAPADAAFIDQ
                     LVVWVRYAFVAALCAVLAARMVRWYWQRRRGIVRLTYPNGRVVQIVHGVSVLEASRMA
                     GIPHASVCGGRGRCSTCRIRVDGAKEAMLPPAAEEVKVLHRVGAAPDVRLACQLRPHG
                     DLRVTPLLPASAQARDGFSRPGYLHGAEREIVILFGDLRSFTQLSEKKLPYDLVFLLN
                     RYFAETGHAVEAAGGRIDKFIGDGVMALFGLDSGVEAGCREALAAAKDMAERMQTLNR
                     ALIHDIAEPLRIGIGIHTGPAIVGEMGYGTAVSITAVGDSVNTASRIEALTKTYACEL
                     VISEAVALRAGIDLGDAPRHEIEIRGRVERLVVRTFASARDLPLLERRGRKKAESAAA
                     V"
BASE COUNT          818 a         1427 c         1468 g          851 t
ORIGIN      
        1 tccttgcaat tttgaacttg ttgcgactga tcccactcaa aattataatg tcggtgtcgg
       61 tcggagcgta gcgcagcccg gttagcgcac cagtctgggg gactgggggt cccgagttca
      121 aatctcggcg ctccgaccaa ctcaaagcca aaaccaaacc gagttcagcg gtcgggtgcc
      181 gcggcgtagg ttagcgaacc gtccggccga ggctgtgccg catcggtcat gcgcgccagg
      241 tcgatgcgat ccttgccgag aatgtggaac acggtctcgc cggcggcgat cagatagtcc
      301 gcaatgatgc gccggtgaca tcgccaccat accgcttccg cgcacatgat tgcgcagcgc
      361 cgttcatgtc ccagcgcgtg cagccgagcg agtccgccat ggaacggttg ggtcagcgtg
      421 tagtcggcat agttgtgaaa gctttggttt tgccagaacg cgttcacgtc gggcggaacg
      481 tcctgcgacc gcgcgcgcaa gccgccgagg gcggcaatgt gctcgtatcc gatctggaat
      541 tgggccagcg tttcgggaag gatatcgcaa ttgtattgcg gattggtgcg cgagcgcggg
      601 acggtgcgga catcgaccac gcatccgacc tgcgctgcct cgagcaggtc gacgaactca
      661 ctcaccggcc gggttgaatg tccgatggtg aagaacggat gcaccatatg tccctggcag
      721 tcgattagcg acgcttgagc ctcagcgcgg ctcccttgtg cagggcgaca tggtcggttt
      781 tatcgctttt gatttcgtac tggggctcat ccttgctcgc gtggtggacg tatcctttgt
      841 aattcacgtc cttcgtatgc accttgacga ttctcccgct gaccctcccg gcctccgagt
      901 tccagcttac acgatcatgc agcttgaatc gttttttcat atcgtctttc gaggattttg
      961 cagagcgaca tcgtactcgc cggcggcgta ctcacgctcg ttgggccgct cgcgtcaagc
     1021 cgaccgacgc aagttccgtc gttgccctaa gggcccgtcg cattggttgc tgggaccatg
     1081 ttgccgtgtg cctgccgtca cggttggagc ggcttcggta ttggccttcg cgtcaaaaag
     1141 caaacgatgt cgctcacccg acggaactcg aaaccgagac ggctgttgag cgtgtgcggg
     1201 acggctgttg agcgtgtgcg gagaatctga ggcagcacgc gcgtctccga ttagaggcct
     1261 cgcgcgaagg agttatctta tgaccgacac agcgattatc gaacgcgatg tggtcgttcc
     1321 agcggcgtct gaagccagcc ggacgccatt ctcctggagc gctgccatcg ccggcgcatt
     1381 ggcggccgcg ggggtgacat ttatcatcgt cgcattgggt tcggggatcg gactgtcgtt
     1441 cgcctcgcct tacgggtctg gcccgtcggc caccagcctg accatcgccg cagccgtgtg
     1501 gctggtcatg gcccagaccc ggctgcgagc gggtctatct cgcttaaagc cgaggagcag
     1561 cgactgatcg tcgcgctcgg acgatttggc ttcgcggcgc gcgcacttgt gttcacgatg
     1621 atcgggcttt ttcttgtgta cgccgcgatc gattccaatt cacgcgaagc aaagggtttt
     1681 gcaggtgcat tgcggcttat tcaacaacag ccccatggat cccttatggc tgggcgttac
     1741 cgccgcgggg ctgctcgcct tcagcattta cgaagtggcg gaagccgcgt ttggccggat
     1801 caccagccct tccctgcgcc aaaccgccgc gaagcgcggg ctcgccggct gatggggagc
     1861 acaagtggtg ttggactttg caagcatgag cgaagacgac ttggcggcgc tgcgaagagc
     1921 cgttgcctcg cttgagcatc caggccttgc cgcacggctc accaatatgg tcggcaagcc
     1981 gatcgagctc attggatacg cgctccccgc ctccgcctcg caggcgatca ccgcagccac
     2041 ctccaaggca ctggagatgg cgctcgaggt tgcgctgcga agcatacacc aggcccctcg
     2101 tgcgggatcg cagctctttc acaaggcctt ggccaccgcc tctggagccg ccggcggcgc
     2161 cttcggtttg gcagcactcc ccatcgaact gcctctctcg accatcatca tgctgaggtc
     2221 gattgcggaa attgcgcgaa gcgagggtga agatctgtcc gatccggaag cggcgctctc
     2281 gtgcgtgcaa gtgtttgcat tgggcggacg gcccggttcg accgatgctt ccgagagcgg
     2341 atattttgcc gtgcgcggca tgctggcaaa aagcgtcacg caggccgcga gatttatcgt
     2401 tgagcgcggc gttatcgagg agggtggccc tattctcgtg cgcttcatta cccaggtcgc
     2461 atcccgcttc ggagtggtgg tcacgcagaa gctcgcggct caagccattc cgctcgtcgg
     2521 cgcgctcgga ggcgctgccg tgaactatgc gttcatcgag catttccagg aagtggcgcg
     2581 aggtcacttt accgttcgtc gcctcgagcg cgcctatggc aaggaggccg tgcagaaagc
     2641 gtatgagcgc ttcgcgcaga acggctgaag acgcggtcgc ccgaggaagc gagcacgcgg
     2701 actccgcgct gcctaattgc tgacgcttgt cggcacagcg tgacgtggag gatgcgactt
     2761 ggtcggcggg tataagtggc cgggtcgaag gactgctggc tttacaccgc tgccgcgctc
     2821 tcggccttct ttcgacctcg gcgctctaga agggggagat cccgcgcgct cgcgaacgtg
     2881 cgcaccacga gccgttcgac ccgcccgcgg atctcgatct cgtggcgagg ggcatctccg
     2941 agatcgatgc ccgcgcgcag cgccacggct tcggagatca cgagctcgca ggcgtaggtc
     3001 ttggtgaggg cttcgatacg gctcgccgtg ttgaccgagt cgccaaccgc cgtgatcgag
     3061 accgcggtgc catagcccat ttcgcccacg atggcggggc cggtatggat gccgatgccg
     3121 atccgcagcg gctcagctat gtcgtggatc agcgcgcgat tgagggtctg catgcgctcg
     3181 gccatgtcct tggccgcggc gagcgcctcg cggcagcccg cctcgacgcc gctgtcgagc
     3241 ccaaacagcg ccatcacccc atcgccgatg aacttgtcga tgcgcccgcc cgctgcctcc
     3301 actgcatggc cagtctcggc aaagtatcga ttgaggagga ataccaagtc gtagggcagc
     3361 ttcttttcgg agagctgggt gaacgacctg agatcgccga acaggatcac gatctcgcgc
     3421 tcggcgccgt ggagatagcc aggacggctg aatccatccc gggcctgggc gctcgccggc
     3481 aagagcggcg tcacgcgcag atcgccgtgc gggcgcagct gacaggcaag ccgcacgtcg
     3541 ggcgctgcgc ccacgcggtg caataccttc acctcttcgg ccgcgggcgg cagcatcgcc
     3601 tccttcgctc cgtcgaccct gatgcggcaa gtggagcagc gcccgcggcc gccgcacacg
     3661 gaggcatgcg gaatgccggc catgcggctc gcctccaaca ccgacacgcc gtgcacgatc
     3721 tgcaccacgc ggccgttcgg ataggtcagc cggacgatgc cgcgccggcg ctgccagtac
     3781 caacgcacca tccgcgctgc cagaaccgca cagagcgccg ccacgaaggc gtaacgcacc
     3841 cacaccacca gctggtcgat gaacgcggcg tccgccggtg ctgcgggcgc atggtcctgt
     3901 gcctgcttgg cgagccaggc cggatcctgg gccagcgcca tgacctgccg gccgccctcg
     3961 agagcgccaa gaattgccaa ggtgggcatc aagagcgcga atgcgtagag gatcggctgc
     4021 cagcgcgggt accacggctt caggcgcagc cagaaccaca gtccgatcat ggcgtggacc
     4081 caggcgacca ccagcaccgc gaactgaagc ccgccacgca gaggattggc cacgaaatag
     4141 gtgtgcagca cgtgcgagta atagccgaca ttggcgccat agaaggtgtc ggcgacgcgc
     4201 gtctggagga cgtgatccgt gaggaaaaac ggaatcgaga agccgagcac gagctgggtt
     4261 gcttcggcga gcggcatctt aagcgagcgg cgcagccaga gcgcccagag cgccaagctg
     4321 tagtgggtcg cgaacgcacc gtagaggagg acgctgccga gggacgacgc ccaatactgg
     4381 tagatgtgct cgagcgcgaa atccatgacc tggaccgaga cgaggcccag gctgtggttg
     4441 acgaaatggg tcgtcacgta tgccaacatg acgagtcccg aagccaaccg cagtcgtcgg
     4501 atcatgcctt ccacccgcgc ccgtcgtcac acgcgttcgc gacttacatc atgacctgcg
     4561 gatg
//
LOCUS       DATLJT010000985         4044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4431965,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000985 DATLJT010000000
VERSION     DATLJT010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4431965"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            733..1332
                     /locus_tag="VM910_22725"
     CDS             733..1332
                     /locus_tag="VM910_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65371.1"
                     /translation="MRTSRKEWWTAVHEAGHAVIGRVLGLECGKVTIVPDFREKTSGV
                     ATVHGQWKTYEAWEKRGKYRDISSVYRGRIIMLMAGAEAEIVCTGRCRGGDDHDRLQI
                     AITMEEVAIPNDDLQRYEQRLRIKTSALIRRHLRKIEHLAKALVEHRTISGRDVDRLL
                     RPVTSPPELERRRRIDKVRREGQATGQLSRISIPPLIQD"
     gene            complement(1395..1571)
                     /locus_tag="VM910_22730"
     CDS             complement(1395..1571)
                     /locus_tag="VM910_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65372.1"
                     /translation="MKRDKLSLDLFWIKDKSLTDTDALQAPDILATEIADDLEAALEQ
                     FTKIAAQLGRHSAA"
     gene            1593..1832
                     /locus_tag="VM910_22735"
     CDS             1593..1832
                     /locus_tag="VM910_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65373.1"
                     /translation="MFTEAQGDAIAHVIVELRKQWRQERDEAIAPLKAEIADLKAKLD
                     AVAELKGKVDALLTLLQGAKAEVIGLPRRRPSDAA"
     gene            1822..2064
                     /locus_tag="VM910_22740"
     CDS             1822..2064
                     /locus_tag="VM910_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65374.1"
                     /translation="MRPEMFLPFLVGYGLAVRQCRTELMATESWLQDEVAELRKGLAA
                     ARAELHRLRAAQRDVTFAPQAPLLLQLNLLMPHGRP"
     gene            2103..3158
                     /locus_tag="VM910_22745"
     CDS             2103..3158
                     /locus_tag="VM910_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVI65375.1"
                     /translation="MTRSRLKQFVLVLGVLISAPTIAAADESGVSFWIPGFFGSLAAA
                     PMQPGWTLQTNYYHTSVSAGADVARSLDIRIGRIPINLNANVSATVDARADLGWAMPL
                     YTFATPVLGGQATVGVLGLYGRVSTSIAGTLAGTLTTPLGTTPFASSANTSDSVSGFG
                     DVLPIATLRWNAGAHNYMTYITGDVPVGAYDSTRISNIGLGHGTIDAGGGYTYFDPQT
                     GHEFSGILGFTYNFENQSDQYQSGVDMHFDWSVSQFVTKQFQIGAVGYVYKEIGCDSG
                     SGDRVGCFQSQVVGVGPQLGFIFPVGDLQGYLNLKYYSEFAAENRPHGWNTWVTFTIS
                     PAVPSPSTSTPKRIITK"
     gene            3314..3562
                     /locus_tag="VM910_22750"
                     /pseudo
     CDS             3314..3562
                     /locus_tag="VM910_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
BASE COUNT          842 a         1174 c         1207 g          821 t
ORIGIN      
        1 gaaaccggct acgcggccgg ggggtcttcg cccgaggagc tgggaacgct gctcaagtcg
       61 gaaatcgtcc gatggagcgc actcgtcaag tcagtcggca tcacactcga ctgatcattg
      121 atgcattggc cacaccacgg cgtttgtggc gtcagatttc gtgaccgcac cggccactca
      181 cgctgctcgc gctggccgat aaagtgatcg aataggatgc tgtttgctgc ggtgcatgag
      241 tcggctcctg acaaagcgga catcatagac gtccgccatt tgtccgcttt cggggcaaag
      301 cggacattga cagggccgcg ctttcaaagc ggtcgcctgc acgtctcgat cttcggccca
      361 tgtcggtgga gcagcccaaa caggcgtacc ataatgaccc gagcaagacg ctggccgcca
      421 cccaggaggc gcgcaatgga tccacgtgac cgagtgaggt cacgcgcgag aattacatcg
      481 ccacgagctg gggcgagcct ctgccggaat ggacggcgga gcttgaggcc gagctgccgg
      541 aagagctgca ggactggtcg ctgttcgaag tcaaaggcca agagctaaag ctcaagaagt
      601 caaaggccaa gaagctaaag ctcaagaaga aacgggggag ctgaagctac tcctgtcgac
      661 atttttgcag ggtctttaga gatgcagtgc cgagtatgga ctgcttcccc agtgtttccc
      721 caggagatga ttgtgaggac atcgcggaaa gaatggtgga ctgccgtcca tgaggcaggg
      781 catgcggtca tcggtcgagt gctgggcttg gaatgcggca aggtaaccat cgttcccgac
      841 tttcgcgaaa agaccagcgg cgtcgccacc gtgcacggcc agtggaagac ttacgaagcg
      901 tgggaaaagc gcggcaagta ccgcgacatc tcgtccgtgt atcgaggtcg gatcatcatg
      961 ctcatggctg gtgccgaagc agagatcgtg tgcacggggc gctgtcgggg aggggacgac
     1021 catgaccgcc tccaaatcgc gatcacgatg gaagaggttg ccatcccgaa cgatgatttg
     1081 caacgctacg agcagcgctt gcggatcaag acgagcgcct tgattagacg ccacctccgc
     1141 aagatcgagc atctcgccaa ggcgcttgtc gagcatcgga cgatctcggg cagggacgtg
     1201 gaccggctgc tgcggccagt aacgagccct ccagagcttg agcggcggcg gcggattgac
     1261 aaggtccgac gcgagggcca agcaacgggc cagctgtccc ggatttcaat tccgccgttg
     1321 atccaagact gacgatcaag ccatgaagct tgttggcaac aaatgtaccc ccgaaattaa
     1381 actgacatga atcgctacgc tgccgaatgg cgtcctaact gcgctgcaat ctttgtaaat
     1441 tgctccaacg ccgcttccaa atcatcggca atctcagtcg ctaagatatc aggcgcctgc
     1501 aatgcatcgg tgtcggtcag gctcttgtcc ttaatccaga agaggtcgag gctgagcttg
     1561 tcacgcttca aaagctcgag ccggaatcgg aattgtttac cgaggcgcaa ggcgacgcca
     1621 tcgcccacgt gatcgtcgag ctgcgcaaac agtggcgcca ggaacgcgac gaggcgatcg
     1681 ccccgctcaa ggccgagatc gctgacttga aggcgaagct cgatgcggtt gccgagttga
     1741 aggggaaggt ggacgcgctg cttaccctcc tccagggcgc caaggccgaa gtgatcggct
     1801 tgcccagaag gcgtccctcc gatgcggcct gagatgttcc tgccatttct cgtgggttac
     1861 gggctcgccg tccgccagtg ccgcaccgaa ttgatggcta ccgagagctg gctgcaggac
     1921 gaggtggccg aattgcgcaa ggggctcgcg gctgctcgtg ccgagttgca tcgcctgcgt
     1981 gctgcgcaac gcgatgtgac atttgcgcca caggcccccc ttctcttgca actgaatttg
     2041 ctaatgccgc acggacgccc ttaatccgat gtcggctctc ccaattattt ggaggccgcg
     2101 ggatgacgcg ttcgaggctc aagcaattcg ttttggttct tggcgtgctc attagcgcgc
     2161 cgacgattgc agccgccgac gaaagtggcg tgagcttctg gatccctggc ttcttcggga
     2221 gcttggcggc ggctcccatg cagccgggct ggactcttca gaccaattac taccatacca
     2281 gcgtatctgc cggggcggac gtggcgcggt cgctcgacat caggatcggt cgcatcccca
     2341 tcaatctcaa cgccaacgtg agcgcaaccg tcgacgcgcg ggctgatctc ggctgggcca
     2401 tgccgctgta tacgttcgcc acaccggttc ttggcggcca ggcgaccgtc ggcgtgctcg
     2461 gactatacgg ccgggtcagc acatcaatcg cggggacgct tgccggcacc ttgacgacgc
     2521 ctttaggaac gaccccgttc gcgtcttccg cgaacaccag cgactcggtc tcgggcttcg
     2581 gagatgttct gccaatagcg acgctgcgct ggaatgccgg cgctcacaac tacatgacct
     2641 atatcactgg ggatgtaccg gtcggggcct acgattcgac ccgcatctcg aatatcggcc
     2701 tcgggcacgg cacgatcgac gccggcggcg gctacaccta tttcgatccg cagaccgggc
     2761 acgaattctc gggcattctc ggcttcacct acaatttcga aaatcagtcg gaccagtatc
     2821 agagcggcgt cgacatgcat tttgattgga gcgtgtcgca gttcgtgacc aagcagttcc
     2881 agatcggcgc ggtaggctac gtctataagg agatcggctg cgacagcggc tccggcgacc
     2941 gcgtcggctg ctttcagtcg caagtggtcg gggttggccc gcagctcggc ttcatctttc
     3001 cggtcggcga cctgcagggc tatctcaatc tgaagtacta ctcggagttt gccgcggaga
     3061 accgtcccca cggctggaat acttgggtca cattcacgat ctcgccggcg gtgccgtctc
     3121 cgagcacatc aaccccaaag cgcattatca cgaaatagct gcgttaggga gatcactgat
     3181 ccagtgaaag gcaccagaac tctgtttgct gttcgctcgt gaacctagtc ggcacctgct
     3241 aatgcactgt caattgaatt ctgcgcgaac acggatatcc cacacctaag tgctcgcaca
     3301 ctagtcgact accgtcgcag ctgcggctgc gaaatacggg acaatgtttg gcgtatcttc
     3361 gctcggcacg gtgagccttg aggagctgcg caaggcgcac gatacaccgc aggtctatca
     3421 gttttatttt tacaaggatc gaggtctcaa ccaagccctg atgcagcggg ccaaggacac
     3481 ctgcatcgaa atcatgatgc tgaccgtaga tagcatcaca ggtgggaacc gcgaacgcga
     3541 tctacgcacg ggcttttcga tgcagatcga tcgtcgctca tgggagattc ccttcctgat
     3601 gatgaggcac gttgtccccc gggtttcgta cctcgtgatt actcacgacg cacgccgggg
     3661 taggagactc ctggcagaac aggaggttac gcctcttgaa ggttgtcacc tccattcaag
     3721 atataacaga atcgtctaca gctttcgtgt cacgtcccag ggtcccggta ctgccggggt
     3781 cgctgggctc gccgtcatcc ggctttccgt ctcactcgcg gccatgctcg ctccttgtac
     3841 tcaagcggtg gatgtatcat atttatgggc ttctacgtga ggcggccaag tctctggaag
     3901 agcgatcatg aaaaaactca ctgtgcttgg cgttatcgtt ggagccgcat tattgagtac
     3961 ggcacccttc tcacttcaat ggtcgcaaaa aagtgtggtg ctgtccctcg acagtgctga
     4021 cgcccgagta ggacgaccgc taac
//
LOCUS       DATLJT010000986         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_983950,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000986 DATLJT010000000
VERSION     DATLJT010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983950"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VM910_22755"
     CDS             complement(<1..122)
                     /locus_tag="VM910_22755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580365.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVI65376.1"
                     /translation="MAENALRNFTLNFGPQHPSAHGVLRLVLELDGEVVERVDP"
     gene            complement(445..2046)
                     /gene="ctaD"
                     /locus_tag="VM910_22760"
                     /pseudo
     CDS             complement(445..2046)
                     /gene="ctaD"
                     /locus_tag="VM910_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
     gene            2379..2837
                     /locus_tag="VM910_22765"
     CDS             2379..2837
                     /locus_tag="VM910_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HVI65377.1"
                     /translation="MSATGLDVFDKTLQTTNIWLDEIMAKLGPDRQVAWHVLGAVLHA
                     LRDRLQIGLAVHLGAQLPLLVRGLYYDQWHPSEQTLKQRSAEQFLDHISRRLADVRPV
                     NIKDATHVTFQVLNHHLDPYQVEKVRRALPENIRALWPAGVRGQPFEPAA"
     gene            2904..>3177
                     /locus_tag="VM910_22770"
     CDS             2904..>3177
                     /locus_tag="VM910_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI65378.1"
                     /translation="MQVSEAMTNDVRIASPNQTIRDAARLMAQIDAGVLPVGENDRLV
                     GMITDRDIAIRAIGADKGPQTPIREIMSKEVMYCFDDDELDDIVQNM"
BASE COUNT          713 a          966 c          971 g          527 t
ORIGIN      
        1 tgcgggtcga cgcgctcgac cacctcgccg tcgagctcca atacgaggcg tagcacgccg
       61 tgagccgacg gatgctgggg cccgaaatta agggtgaaat tgcgcagggc attttcggcc
      121 atcacggcct cgcgtcagcc ccaggttcaa cgccggcccg ccgacagcgg ttccaacttc
      181 gcccccgccc gttcggatcc cttgaggaac acgccgcaag gcggagctaa acggcgatgg
      241 ggctcagcgg cgatgaaaat cgccggcagg cggccgcggt tcgagacttg gacgtttgag
      301 acttggacgt ttgagacttg ggcgtttact ggagctcgca acacttgcgc cttcgacact
      361 ttggccttcg acacttgcgc cctcaacgcc tgagctctca acgtttgggt tttcaagact
      421 cgagccgtcg aggctcggag cgacgatccg cggcagggtg tcgaactgat ggaacggcgg
      481 cggcgagggt aaggtccatt caagcgtggt tgcgcccgga ccccagggat tggcgggcgc
      541 cggcgctttg cgcacaaatg caagaatcat attggcaaag aagacggcca tgccggccgc
      601 gaacacgtag gcgcccaacg atgaaatgaa gttccagtca ctgaaggcgt cggggtagtc
      661 cgccacccgc cgcggcatgc ctgcgagccc gagaaaatgc tgcggaaaga agatcgtgtt
      721 ggcaccgacg aacgaaaccc aaaagtgaac cttgccgaga aaatcggaat agctgtagcc
      781 ggtcatcttc gggaaccagt agtaccagcc ggcaaagatg ccgaagacag cagcaagcga
      841 cagcacatag tggaagtgtg ccacgatgta ataggtatct tgcagcatac ggtcggcgcc
      901 cggattggca accacgacgc cggtgagccc gccgatcgta aacatgaaga tgaagccgat
      961 cgcccacagc atcggggtgc ggaattcgac cgaaccgccc cacatcgtcg cgatccagga
     1021 aaagacctta acgccggttg gcaccgcgat caccatgctt gccaccgcga aataggcctg
     1081 cgcgccggcc gtcatgccca ccgtatacat atggtgcgcc cacaccacga aaccgatcgc
     1141 accgatcgcg atcatggcaa acaccatgcc gagatagccg aacaccggct tgcggctgaa
     1201 ggtagcgacc acctggctga caatgccgaa agccggcagg atcatgatgt agacttcggg
     1261 atgaccgaag aaccagaaca agtgctggtg caggagtgga tcaccgcccc cctgaggatc
     1321 gaagaaggtg gtgccgaaat tgcgatcggt gagcagcatc gtcagcgttc ccgcgaggac
     1381 cggcatcgcc ggcaatatca ggaacgccgt gatcaagatt gaccacacga acagcggcat
     1441 ccgcagcagg atcatccccg gcgcgcgcat gttgaagatg gtggtgatga agttgattgc
     1501 gccgaggatc gaggaggcgc ccgccaggtg tagcgacagg atcacgaaat cgaccgccgc
     1561 gccgggatgg cccctggtcg acaacggcgc atagagcgtc cagccggttc cagccccgct
     1621 cgtgctgggc tcacccgtca tcaacgaaga catcagcaat agcgtgatcg aggccggcag
     1681 cagccagaac gagatgttgt tcatgcgcgg gaacgccata tcgggcgccc cgatcatcag
     1741 cggcaccatc caattgccga agcctccaat tagagccggc atcaccatga aaaagatcat
     1801 gatgaggccg tgcgcggtcg tgaacacatc gtaggcgtgc gaactcgcaa agatctgcag
     1861 accgggctgc tgcagttcca tccgcatccc gatcgacagc gcgccgccga tgatccccgc
     1921 caggagggca aacgcgaggt acatcgtgcc gatgtctttg tgattagtgg agtaaaggta
     1981 gcggcgccat ccgctcggat gatcgccgat gtggtgagtc ccgtgggcga cgccagcagc
     2041 tgccatggtc catcccttta atggtgggat gtcgccccac tacagttccg atgttccggt
     2101 ccgcgccgaa ggagggctct cggccatatg aggtggcctg cagccattct tgccgagcag
     2161 gtttccctct cctgtcaaac ctcgcaacga tccgacggtt ccgatgagga gaccgtcgtc
     2221 ggaaaatcag ttagcggaaa aacggcggag gaactttcag ccgatttccg cgatgccgga
     2281 acttttctgc tgaccgaaaa ccgcatcgac gcatcggaac cgcgccctgc accgtgcgtt
     2341 ccccgagaga accgcggtgc gagcatggga ggactgacat gagcgccacc ggtctcgatg
     2401 tgttcgacaa gactctgcag accaccaaca tctggctcga cgagatcatg gccaagctcg
     2461 ggcccgaccg acaggttgca tggcatgtgc tcggtgccgt tctgcacgcg ctgcgcgatc
     2521 gcctccagat cgggctagca gtccatctcg gcgcgcaatt gccgctgttg gtgcgcggtc
     2581 tctactacga ccaatggcac ccgagcgagc agacgctcaa gcaacggtcg gcggagcagt
     2641 tcctggacca catatcgcgg cgtctggcag acgtgcggcc ggtcaatatc aaggacgcca
     2701 cacacgtgac cttccaggtt ctcaaccatc acctcgatcc gtaccaggtc gaaaaggtgc
     2761 gccgcgcgct gccggagaac atccgcgcct tgtggccagc cggtgtacgg gggcaaccct
     2821 tcgaacctgc cgcttgacgc ggccccggcg gcccaacttc gcgcgtcggt tccaaagcgg
     2881 ttcaacacgt gaggagaaat accatgcaag tcagtgaagc gatgaccaac gacgtcagga
     2941 ttgccagtcc caatcagacg atccgcgatg ccgcgcgctt gatggcacag atcgacgctg
     3001 gggtgctgcc ggtcggagag aacgacagat tggtcggcat gatcaccgac cgcgacattg
     3061 ccattcgcgc gatcggcgcc gacaaaggcc cgcaaacgcc gatccgggag atcatgagta
     3121 aagaggtcat gtattgcttc gatgacgacg aactcgacga catcgtgcag aacatgg
//
LOCUS       DATLJT010000987         5300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1215102,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000987 DATLJT010000000
VERSION     DATLJT010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215102"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="lnt"
                     /locus_tag="VM910_22775"
     CDS             <1..637
                     /gene="lnt"
                     /locus_tag="VM910_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVI65379.1"
                     /translation="VRAPDLPPGVRVTRDYNSIYVIDHDGSVLSVYDKLHLVPFGEYL
                     PYQDWLERLGIEQLTKVQGGFIPGTVRRSMPVPNAPRVLPLICYEAIFPGDVATRDDR
                     PGWIINLTNDGWFGISTGPYQHLQQARMRAIEQGLPLVRAANTGISAVIDPLGRIVAR
                     LGLGIEGVLDSDLPSAGAPTIYSRVGDWPAAMIVAIALIFVIGRRFANSKL"
     gene            892..1308
                     /locus_tag="VM910_22780"
     CDS             892..1308
                     /locus_tag="VM910_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI65380.1"
                     /translation="MSIKAPNPVDKYVGSRVRMRRIMLGMSQEKLGEALGLTFQQIQK
                     YEKGTNRVGASRIQQISEILQVPVSFLFEGGPSGAAGTDGFSEGASPTYVSDFLATSE
                     GLALTRAFTRITDAKLRRSIVDLVEQIAARETPEKR"
     gene            1428..2555
                     /locus_tag="VM910_22785"
     CDS             1428..2555
                     /locus_tag="VM910_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVI65381.1"
                     /translation="MTATNPFDAQAILDGIRQWVEIETPTEAPEQVNRLATLVASGYR
                     DLPATIERVAGKDGCGDHLVARSAWGQDAPGILVLSHLDTVHPLGFIERLPFRIEGDS
                     AFGPGIYDMKGGAYLAYHAFRRLCANPTRSPLGVTQFYVSDEEIGSPTSRALIEAEGR
                     KAKYVLVTEPARDGGKIVTGRKGVGRFEVFVKGIPAHAGSRPEDGRSAIRELANVIQT
                     LEAMNDAKRGITVNVGVVRGGTRPNVIAEEAYAEVDLRVPTAADAGEFVGKILNLESR
                     SDGVSVRVTGELNRPPYEKGNAGAALFGHARTLAAEIGFELNDTFTGGGSDGNFTAPY
                     TATLDGLGVDGKGAHTHYEQMFISSIEPRARLLYRLYQTLR"
     gene            2552..3289
                     /gene="trmB"
                     /locus_tag="VM910_22790"
     CDS             2552..3289
                     /gene="trmB"
                     /locus_tag="VM910_22790"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HVI65382.1"
                     /translation="MSAPRGDHPKTDPSGQGPRPGHGSFFGRRKGHRLRAHQTGLIEN
                     LLPQLALDTGGPAPVEAGILFVPQAQDVRLEIGFGGGEHLVAEAHNAPDAGFIGCEPY
                     VNGMAKILAQIESRGVRNIRLFAGDAADLLAWLPPRSLSRIDLIHPDPWPKRRHWKRR
                     FVQDATTAAMARVLKPQGEFRFVSDIAEYTAWTLWHLARSPHFAWIAERADDWRQPWP
                     GYTITRYGQKATREGRPAAYLRFQRVD"
     gene            complement(3290..4138)
                     /locus_tag="VM910_22795"
     CDS             complement(3290..4138)
                     /locus_tag="VM910_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVI65383.1"
                     /translation="MLPEDAIQSHATRIRDEGYTVVERAAAPALVAGSKEALERIERE
                     HNLASAKTSFEGFKTLRINNLLTYDDIFWQVPLHENVLPIVERVLDKECLLSSFCSLV
                     LGPGQEAQPIHEDTQLIPLPRPHIPITVNAIWALSDFRHDNGATRIVPGSHKFESSPE
                     YGRAYDAVTATMPAGSVMLFDSALWHGGGANVSDERRFAFSCAYCWGWMRQQENLQLG
                     IPRDAARRFPRRLQELCGYSVYKGQFGHIDNHDPIELLGRERGKRMVWEATDVKKARM
                     ADAAKP"
     gene            complement(4208..5293)
                     /locus_tag="VM910_22800"
     CDS             complement(4208..5293)
                     /locus_tag="VM910_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HVI65384.1"
                     /translation="MTVATSLVPGLDDIVKNGDPKRRTEAARRVADLFFQDAASLRPR
                     QVELLDEILIDLVPQAELIARAELAERLSLFSNAPRVLVGRLARENEILVAGPLLRRS
                     PVIDEEVLLEIAQARGQGHLLAMAERSALSPDLTDVMLRRGDRDVVRRTAGNNGALFS
                     ASGYSELIKRASQDGVLTITVGQRNDLSDQMLKQLLAGSLDVIRRRLFDVVKPERQAA
                     IKQAMADITGVSERHESKRDFVPAQRTILELHDAGNLNEAALLSFAKAYRYEECVAAL
                     SALSGVKIETLDNLISNDRHDPILILGKTIGLEWATVRALILLRSGPNRVPAPADIES
                     ARMNFSRLMPSTAERVVGFWKTRFAPY"
BASE COUNT          962 a         1704 c         1656 g          978 t
ORIGIN      
        1 cgtgcgcgcg cccgatttgc cgcccggcgt ccgagtcacg cgcgactaca attcgatata
       61 tgtcatcgat cacgatggca gcgtgctctc ggtttacgac aagctgcacc tggtcccgtt
      121 cggcgagtac ctgccctacc aggactggct ggaacggttg ggcatcgaac aactcaccaa
      181 ggtgcaaggc ggattcattc ccggcacggt gcgtcgctcg atgccggttc cgaacgcgcc
      241 gcgtgtgcta ccgctgatct gttacgaggc gatctttccg ggcgacgtcg ccacgcgcga
      301 cgaccggccg ggctggatca tcaacctgac caatgacggc tggttcggga tctccaccgg
      361 tccctatcag catcttcagc aggcgcggat gcgcgccatc gagcagggct tgccccttgt
      421 gcgcgccgca aacaccggca tctcggcggt gatcgatccg ctgggacgaa ttgtcgcgcg
      481 gctcggcctc ggcattgaag gcgtgctcga ctccgatctg ccgtcggctg gcgccccgac
      541 catctattcg cgcgtcggcg attggcccgc ggcgatgatt gtcgcaattg ccctcatatt
      601 cgtcatcggc cggcgtttcg caaacagcaa attgtgacga cggcgtgttc atgcttgaag
      661 atttacgttt ggttttcgat ttccgtcgaa ttttacgaac cgcgaattcc agcagttgac
      721 agacctgtcc tgccgttcgc attctgtcgc cgaccactta gctccagtca gttcgttgct
      781 ccaaagtctg caatcctatc cagcctccga gcaaggtttg acggtgttgg cgtcggagac
      841 aggcgtggct tggtccgcct ctctcccgcc cgatcgcaag gagagttggc aatgtcgatc
      901 aaggccccga accctgttga caaatatgtc ggcagtcggg tgcggatgcg ccgcatcatg
      961 ctgggcatga gccaggagaa gctgggcgaa gccttggggc tcacgttcca gcagatccag
     1021 aaatacgaaa aaggtaccaa ccgggtcggc gcaagccgca tccaacagat ttccgaaatc
     1081 ctacaggtgc cggtctcgtt cctgttcgag ggcggcccaa gcggtgccgc aggcaccgat
     1141 ggcttcagcg agggcgcctc gccgacttac gtgtcggatt ttctcgcaac ctcggaggga
     1201 ttggccctga cgcgggcgtt cacgcggatc accgatgcca agctcaggcg cagcatcgtc
     1261 gatctcgtcg agcagatcgc cgcgcgcgaa acccccgaga aacgctgatt cttcgccgtt
     1321 tgggtttgag cgtccggaaa atctggacta tgccgtgatt ccggcgggca cgagtgagcc
     1381 ggccgctcgt gacggcgttc gagcttcagc agggctgcat ccgaccgatg accgcaacca
     1441 atccgttcga tgcgcaagcc atcctcgacg gcatccgcca atgggtcgag atcgagacgc
     1501 cgacggaggc gcccgaacag gtcaacagac tcgcaacgct ggtcgcgtcc ggctatcgcg
     1561 acttgccggc gacgatcgag cgcgttgcgg gcaaggatgg ctgcggcgat cacctggtgg
     1621 cgcgttcggc ctggggacag gatgcgccgg gcattctggt cctgagccat ctcgataccg
     1681 tacatccgct cgggttcatc gaacgcctgc cctttcgcat cgaaggcgac agcgccttcg
     1741 gccccggcat ctacgacatg aagggcggcg cctatctcgc gtaccacgca ttccgacggc
     1801 tttgtgccaa tcccacccgc tcgccgctgg gcgtgaccca gttctacgtc tccgatgagg
     1861 agatcggcag tccgacctcg cgcgcactga tcgaggcgga aggacgcaag gcaaaatacg
     1921 tgctggtgac ggagccggcc cgcgacggcg gcaagatcgt gacgggacgc aagggcgtcg
     1981 gacggttcga ggtcttcgtg aagggtattc cggcgcatgc cgggtcacga ccggaggacg
     2041 gccgcagcgc aatccgcgaa ctcgccaatg tcattcagac gctggaagcg atgaacgacg
     2101 caaaacgcgg catcaccgtc aatgtcggcg tggtcagggg gggcacgcga ccgaacgtga
     2161 ttgcggagga agcttacgcg gaggtcgacc tgcgggtgcc gactgctgcc gacgccggcg
     2221 agttcgtcgg aaaaatcctc aatctggaat cgcgaagcga cggcgtcagc gtgagagtga
     2281 cgggtgaatt gaaccggccg ccatacgaga agggcaatgc cggcgccgcc ttattcgggc
     2341 acgcaagaac gctcgctgcg gaaatcggct tcgaactcaa cgacacgttc acgggcgggg
     2401 gctctgacgg caatttcacc gcgccctaca cagcaacgct cgacggcctc ggcgttgacg
     2461 gcaagggcgc gcatacccat tacgagcaga tgttcatctc ctcgatcgag ccgcgggcgc
     2521 ggctgctcta ccggctgtac cagacgctgc gatgagcgcg ccgcgcggcg atcacccgaa
     2581 aaccgatccc tccgggcagg gcccgcgacc agggcacggc tcgttcttcg gccgccgcaa
     2641 gggacacagg ctgcgtgcgc atcagaccgg cctgatcgaa aacctcctgc cgcaactcgc
     2701 gctcgacacc ggcggtccgg caccggtcga ggccggcatt cttttcgttc cacaagcaca
     2761 agatgtcagg ctcgagatcg gcttcggcgg cggcgagcat ctggttgcgg aggcgcacaa
     2821 tgcacccgac gccggcttca tcggctgcga gccctatgtc aacggcatgg ccaagatcct
     2881 ggcgcagatc gaaagccgcg gcgtgcggaa tatccgcctg tttgccggtg atgccgcaga
     2941 cctgctggcc tggctgccgc cgcgctcgct gtcacggatc gacctgattc atcccgatcc
     3001 ctggccgaag cggcggcact ggaagcggcg ttttgtgcaa gacgcaacga ctgccgcgat
     3061 ggcgcgcgtg ctgaagccac agggcgaatt ccgcttcgtc agcgacatcg cggagtacac
     3121 tgcgtggacg ctgtggcatc ttgcgcgctc acctcacttc gcctggattg cggagcgcgc
     3181 cgacgactgg cggcagccat ggcccggcta caccatcacg cgctatgggc agaaagctac
     3241 gcgcgagggc cggcccgccg cttatttgcg gtttcaacgc gtggattagc tacggctttg
     3301 ctgcgtcggc catccgcgcc tttttcacgt cggtcgcttc ccacaccatg cgttttccgc
     3361 gctcgcggcc caggagctcg atcggatcgt gattgtcgat gtgcccgaac tggcccttgt
     3421 agacgctgta gccgcacagc tcctgcagcc gccgcgggaa tcttcgcgcg gcatcccggg
     3481 gaatgccgag ctgcaggttt tcctgctgcc gcatccagcc ccagcaataa gcgcaggaga
     3541 acgcgaaccg cctttcgtcg ctgacattcg cgccgccgcc gtgccacaac gcgctgtcga
     3601 acagcatcac gctgccggcc ggcatcgtgg cggtcaccgc gtcgtacgcc ctgccgtatt
     3661 cgggcgagga ctcgaatttg tggctgcccg gcacgatacg cgtcgcgccg ttgtcgtggc
     3721 ggaaatccga cagcgcccag atcgcattca ccgtgatcgg aatgtgcgga cgtggcagcg
     3781 gaatgagctg ggtgtcttca tggatcggct gtgcttcctg tccggggccc agcaccaggg
     3841 agcagaacga cgacaacagg cactctttgt ccagcacgcg ctccacgatc ggaagcacgt
     3901 tctcgtgcag cggcacttgc cagaagatat cgtcgtacgt caaaaggttg ttgatgcgca
     3961 gcgtcttgaa gccttcgaac gacgtcttgg ccgatgcgag attatgctcg cgctcgatgc
     4021 gctccagcgc ctctttcgat ccggcaacga gcgcaggagc agcagcccgc tcgaccacgg
     4081 tgtatccctc gtcgcggatc cgcgtcgcgt gcgattgaat ggcgtcttcc ggcagcatgg
     4141 tttttcctcc ctgctcacgc cgctttcctg cgagcatagc gcgccggttt cgggagagga
     4201 aagtccctca atacggcgcg aagcgggtct tccagaaacc cactacgcgc tcggcagtcg
     4261 atggcatcaa gcgcgagaaa ttcatccgcg cgctctcgat gtcggcaggc gccggcacgc
     4321 ggttcggccc cgaccgaagc aggatcagcg cacgcacggt cgcccattcg aggccgatgg
     4381 tcttgccgag gatcaggatc ggatcatgac ggtcgttcga aatcagattg tcgagggtct
     4441 cgatcttcac tcccgacagc gccgacagcg ccgcaacaca ttcctcgtag cgataggctt
     4501 tcgcgaaaga gagcagcgcg gcctcgttga gattgcctgc gtcatgcaat tccaggatgg
     4561 tgcgctgtgc cggcacgaaa tcccgcttgc tctcgtgccg ctcggaaacg ccggtaatat
     4621 cggccatcgc ctgcttgatc gcggcctgcc gctccggctt gacgacatcg aacaggcggc
     4681 ggcggatcac gtcgagcgac ccggccaaaa gctgcttcaa catttggtcg gagagatcgt
     4741 tacgctgacc gacggtgatc gtcagcacgc cgtcctggct tgcgcgcttg atcagttccg
     4801 aatatccgct tgcagaaaac agggcaccgt tattgccggc cgtgcgacgc acgacgtcgc
     4861 ggtcgccgcg gcggagcatc acgtcggtca ggtcaggcga aagcgccgac cgttcggcca
     4921 tcgcgagcag atgaccctgt cccctggctt gcgcgatttc gagcagcacc tcctcgtcga
     4981 tgaccgggga ccgtcgcagc agaggacccg ccaccaggat ttcgttctcg cgggccagtc
     5041 gcccgaccag caccctcggc gcattggaaa acagcgacag ccgctcggcg agttcggctc
     5101 gcgcgatcag ttctgcctgc gggaccaggt cgatcaggat ctcgtcgaga agttcgacct
     5161 gcctgggacg caagctcgcg gcgtcctgaa aaaacagatc ggcgacgcgc cgcgcggctt
     5221 ccgtacgtcg tttcggatcg ccatttttga cgatgtcatc gagtccgggg accagcgacg
     5281 tggctactgt catcgaatct
//
LOCUS       DATLJT010000988         1570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_66573,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000988 DATLJT010000000
VERSION     DATLJT010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66573"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VM910_22805"
     CDS             <1..965
                     /locus_tag="VM910_22805"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65385.1"
                     /translation="NRPVRIYRLPDAVPKPQPSATAEPALRHIGFLGSMRRPVYDEFY
                     RRLAAYGYVDGQTIRIHHRWCEGDYAKYPELVKQLIDIPVELIITTETPSTLAAKQAT
                     STVPIVMIEVGDPVGYGVVPSLTRPGGNVTGLSNNLHEYGPRCLRFFKEIMLGASRLA
                     ILAPTIGPPAEAWVKSVEAVSLALEMVPKVYYSGNADDLRHVLAGIDPRSDVLFAAPP
                     FGLVPQRRTAIIAATMQLKMPVISQQPEFVLDGALLSFDPNRTEIYRRLAYYVDAILK
                     GAQPSALPIEEPNKSWLMINLRTAKTLGIEIPGPILIRADQVIE"
     gene            complement(1205..>1570)
                     /locus_tag="VM910_22810"
     CDS             complement(1205..>1570)
                     /locus_tag="VM910_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI65386.1"
                     /translation="KQLIETVETLRLRGIGFRSLTEALDTTTAQGRLVFHMFGALAEF
                     ERSLIRERTQAGLAAARRAGRTGGRPPKLTDDDIEAAKTMLANPDIGVTQIAHPLGVS
                     PATLYRYMPAARTANTPDV"
BASE COUNT          303 a          478 c          487 g          302 t
ORIGIN      
        1 tcaatcgtcc ggtccgcatc taccggctac ccgacgcggt accgaagccc cagccctccg
       61 cgacggcgga gccagccttg cgccacatcg ggtttctcgg cagcatgagg cgcccagtat
      121 acgacgaatt ttaccgccgc ctcgccgctt acggatacgt cgacggccag acgatccgaa
      181 ttcaccaccg ctggtgcgaa ggtgactatg cgaagtatcc cgaactcgtc aaacagctca
      241 ttgatatccc ggttgagctg atcatcacga cggaaactcc cagtacgctc gcggcaaaac
      301 aggcaacgag cactgttcct atcgtaatga tcgaagtcgg cgatccggtg ggctatgggg
      361 tcgttccgag cttgacgcgg cccggtggga acgtgaccgg cttgtcgaac aatctgcacg
      421 aatacgggcc aaggtgcctg cggttcttta aggaaatcat gctgggggcg tcgcgcctgg
      481 caattcttgc cccgacaatc ggtcctcctg cggaagcttg ggtcaagtcg gtcgaggcag
      541 tgtcgctggc actggagatg gtgccgaagg tgtattactc aggcaatgcg gacgaccttc
      601 gccacgttct tgccggcatc gatccgcgga gcgatgttct ttttgcggct cccccgttcg
      661 gcctcgtgcc ccaacggcgg acggccatca tcgcggccac catgcagctg aagatgccgg
      721 tgatctcgca acagccggaa ttcgtcctgg acggcgcgct gctttcgttc gatcccaacc
      781 ggacagagat ttaccgacgg cttgcctact atgtcgatgc gatcctcaaa ggcgcacagc
      841 cgagcgcgct gccgatcgaa gagccgaaca agagctggct gatgatcaac ttacggacgg
      901 ccaagacgct gggtatcgaa ataccgggcc cgatcttgat acgcgcggat caggtgatcg
      961 aataaagccc taacccgtcc ccctcacttc gggtggactg gtcacgccag gccggcatca
     1021 gaaatcttac ctggaataag gcatgggctg gattgggatc gaggaaagcg aaacggcagc
     1081 tttgggtcga acctcaccgt tccgggatga cgacgggagg gtcagcaaac ggcgcactgc
     1141 cgccgcccgc cgccaggctg aagccttcct ggagcgtccc atctttggga taaagcggcc
     1201 gttctcaaac gtctggggta ttcgcggtcc gcgcggcggg catatatcga tacagcgtcg
     1261 ccggcgagac gccgaggggg tgtgcgatct gagtcacgcc gatgtcggga ttggcgagca
     1321 tcgtcttggc tgcctcgatg tcgtcgtcgg taagttttgg cggacggcct ccggtgcggc
     1381 ccgcgcggcg ggcggcggca aggccggctt gagtgcgctc acggatcaga ctgcgctcga
     1441 attccgccag tgcgccgaac atgtgaaaaa ccagccgccc ctgcgcggtc gtcgtgtcga
     1501 gcgcttcggt caggctgcga aacccgatac ctcttagccg cagggtttcg acggtttcga
     1561 tcaactgctt
//
LOCUS       DATLJT010000989         1609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3513200,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000989 DATLJT010000000
VERSION     DATLJT010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3513200"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..674)
                     /locus_tag="VM910_22815"
     CDS             complement(405..674)
                     /locus_tag="VM910_22815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65387.1"
                     /translation="MAARHKLPAVFPGRFYVTSGGLISYGPDLVDQFRRAAGYVDRIL
                     KGERPADLPVQAPTRYELVINLKAAKALGLEVPATVLARADEVIE"
     gene            complement(683..1387)
                     /locus_tag="VM910_22820"
     CDS             complement(683..1387)
                     /locus_tag="VM910_22820"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65388.1"
                     /translation="MNRRQCISLLGSAAAWPLAARAQQGERVRRIGVLLYLVAGDAEG
                     QARLGAFTQALSQLGWSEGRSLRIDTRWATADDVHRHAAELVALTPDVLLAATGTATV
                     APLLQATRTIPIVFVNVIDPIGSGFVASLAHPGGNATGFTIYEFSMSGKWLELLKEIA
                     PGVTRAAVLRDPAVASGIGQFGAVQIVAPALGVQLTPVDVRDAGAIERAVAAFATGSN
                     GGLIVTGSALGSSIAS"
     gene            complement(1384..>1609)
                     /locus_tag="VM910_22825"
     CDS             complement(1384..>1609)
                     /locus_tag="VM910_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65389.1"
                     /translation="MAEAGCLFSYSASIKDMWYRSASFVDKILKGAKPADLPVQQGTK
                     FEFVINLKTANALGLDVPPTLLTRADEVIE"
BASE COUNT          271 a          529 c          509 g          300 t
ORIGIN      
        1 tagttcgcaa gctagtgacc aagagtgccc cgcacgtcgg ccagagcctg ctcgcatgct
       61 gccttgtcgc cagcgtcatc ggctgcacgc gcacgcgcca tggcttgcct aatagcattg
      121 accgtctgag gcgatatttc gcccatcctt tcctcaacag tttccagcga acgcggcgtg
      181 ggttgagggc ttgttccggc catggcttcc gcagtcgcag gtggcccgtc ggcagctttt
      241 gcttcgagca cggcatcgat gcttgcctgc acggcggaga tgtcgtccca acatgggccg
      301 ccgtaactcg ctgaaatcga ggcggccagc gtagctgcgc acgctgctat cgatcgcatt
      361 ctcataactc tatctcccca agtgcgaggt ttggcctaag tacgtcactc gatcacctcg
      421 tcggcgcggg cgagcaccgt cgctggcact tcgaggccaa gcgccttggc ggctttgagg
      481 tttatgacga gttcgtacct ggtcggcgcc tggacgggaa gatccgcggg cctctcgccc
      541 ttgaggatgc gatcgacgta gccggccgcg cgccggaact ggtcgacaag atcggggccg
      601 taggagatca ggccgccgct ggtgacgtag aaacggccgg ggaagaccgc gggcagcttg
      661 tgccgtgccg ccacttgggc gatcagctcg cgatggacga ccccaatgcg cttccagtca
      721 cgatcaggcc gccattcgac cctgttgcga atgccgccac ggcgcgctcg attgccccgg
      781 catcgcgcac gtcgaccgga gtcaactgca cgcccaatgc cggcgcgacg atctgcaccg
      841 cgccgaactg cccgattccc gaagcgacgg ccggatcgcg aagcaccgcc gctcgcgtca
      901 cgcccggcgc gatctccttg agcagctcca gccatttccc gctcatgctg aattcgtaga
      961 tggtgaaccc ggtcgcgttg ccgcccggat gtgccaggct cgcgacgaaa ccggagccga
     1021 tcggatcgat gacattcacg aacacgatcg gaatggttcg cgtcgcctgc agcaacggtg
     1081 ccacggttgc ggtgccggtc gcagccagta ggacgtcggg tgtgagcgcg accagttctg
     1141 ccgcgtgtct gtgaacatcg tcggcagtgg cccagcgggt atctatccgc aggctgcggc
     1201 cctcactcca tcccaattgg ctgagcgctt gtgtgaacgc cccgagccgg gcttgtccct
     1261 cggcatcgcc agcgaccaga tataggagca cgccgatgcg ccgaacgcgc tcgccttgct
     1321 gcgctcgcgc cgcgagcggc cacgccgccg cgctgccgag cagcgatatg cactgacggc
     1381 ggttcactcg atcacctcgt cggcgcgggt gagcaatgtg ggcggcacat cgagaccgag
     1441 cgcgttggcg gtcttgaggt tgatgacgaa ctcgaacttg gtcccctgct gcaccggcag
     1501 gtcggctggt tttgcccctt tgaggatttt gtcgacgaag ctggccgagc ggtaccacat
     1561 gtctttgatg ctagccgaat aggagaacag gcaacccgcc tcggccatc
//
LOCUS       DATLJT010000990         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3743100,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000990 DATLJT010000000
VERSION     DATLJT010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743100"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1182)
                     /locus_tag="VM910_22830"
     CDS             complement(202..1182)
                     /locus_tag="VM910_22830"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HVI65390.1"
                     /translation="MDFGAWWSYVYPRLSGYELREEEAKKGRGAWTFGSKERKEFAEF
                     YAAEASAAATASQGGTSTTRLYGLTCTASVKYVGHTAIKRDIENLAAAVRSTRVEEAF
                     MTAVSPATLQILPNAYYKNAEDYTWALAEAIREEYRAIVDAGFIIQIDDPALVDIYDW
                     WFSMNDDIAGFRKWAAFQVEAVNHALKGIPEDRVRFHICWGSWHGPHKGDVGLKDVVD
                     LLLKVKAQAYVIEAANVRHEHEWKIWKTTRLPEGKLLIPGVVSHATNVLEHPELIADR
                     LVRFAQTVGRENVIAGTDCGLGGRVHPQIAWAKLEALSEGAKLASRQLWL"
     gene            complement(1412..2650)
                     /locus_tag="VM910_22835"
     CDS             complement(1412..2650)
                     /locus_tag="VM910_22835"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HVI65391.1"
                     /translation="MRHSKDRILTTHAGSLSRPANLIALSKSIAAGETKGDDAAYAQV
                     LASAVADVVKKQHELGVDIPDDGEFGKPVDGSYDYGAWWNYAFARMSGFSPPGSPAAA
                     AHETQHKKSSVADLALTSFANRRDWQRFNEFYQDPESSGSLMGSAARRPVGRPACTGP
                     IKYIGHAALQADIANLKKALAATGTEEGFMCSIGPGSFARGVDLYYKTEEEFLFASAD
                     ALHEEYKAIVDAGVVLQIDDPSLPDNWDMINPEPPLREFKKFEQVRMEALNHALRGLS
                     ADRIRFHICWGSWHGPHTTDIPLKDIVDLVLSVNAGAYSVEAGNVRHEHEWRVWQDVK
                     LPDGKLLIPGVVSHATNIVEHPQVVADRIVRYAKVVGRENVIAGTDCGLGGRIHPQIA
                     WAKLQALTEGAALATKELWG"
     gene            2791..>3915
                     /locus_tag="VM910_22840"
     CDS             2791..>3915
                     /locus_tag="VM910_22840"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65392.1"
                     /translation="MTDTRLSRRTMLEGATALGVLAVTSGETFGQQTVSRSAAAPDTP
                     LPPRREFVIRGASVLTMDPNIGDFATGDVHVRDGAIVAVAPRIDVANAQIIEARDMIC
                     MPGFVDTHWHLWTSLLRPFIRADVDELGYFPVSFRLGQLFTPEDSYRSVLLGLAEALS
                     AGVTTVYNWAHNVRSPDHADAELSAMRDAGIRGRFAYGPAQGMPDNQPMDMTGLARVK
                     RDWMPGNGLLTLGICSRNIGAMSIGGATRGTLTVEMAKRDWGGARALGLPITLHTSGA
                     SPIKLLEEAGLLGSDVQLVHPLLTTPEERAILKARGVSYATAPVGEARRPSSVGVIQL
                     GELLEAGVKVSLSTDHTTNYNCDPFVAMRILFALHQHRIGN"
BASE COUNT          731 a         1221 c         1166 g          797 t
ORIGIN      
        1 ggcctcacct ccttgaatcg ctctggcttt gacgcaacca gtctatagca cttcgatgcc
       61 gcagagtggg cgccgtccac agcatcagtc gcgccggata gccactccgc gcgcggtcat
      121 ggcatgtccg cttcgccgca aaagcgacca tcggtgatgc cccatttcag tgcggcttcg
      181 gtgagcggcg cggcatcggc gctagagcca aagctgccgg ctcgccagct ttgcgccttc
      241 agacaaagcc tctagtttcg cccacgcaat ttgcggatga acccgcccgc cgagaccgca
      301 gtcggttccg gcaatcacat tctcacggcc gacggtctgc gcgaagcgga caagccggtc
      361 ggcaatgagt tcgggatgtt cgagaacatt ggtggcgtgg ctcacgacgc ccggaataag
      421 aagtttaccc tcgggcagcc tggtcgtttt ccagattttc cactcgtgct catggcgaac
      481 attggcggct tcgatcacgt aggcctgcgc ctttaccttg agcagaaggt caaccacgtc
      541 cttgagcccg acatctccct tgtgcgggcc atgccagctg ccccagcaga tatggaaacg
      601 aacgcggtct tcggggatac ctttgagggc gtggttcacg gcctcaacct gaaacgccgc
      661 ccatttgcga aaacctgcga tgtcgtcgtt cattgagaac caccaatcgt agatgtcgac
      721 tagcgccgga tcatcaattt ggatgataaa accagcgtcg acgatggctc tgtattcctc
      781 acgaatcgcc tcggccaaag cccaggtgta atcctccgca tttttgtaat acgcattcgg
      841 cagtatctga agtgtcgccg gcgacaccgc cgtcataaaa gcctcttcca cacgagtgga
      901 gcgaaccgcg gcggccaggt tttcgatgtc gcgcttgatg gcggtatggc cgacatactt
      961 aactgaagcg gtgcaggtga ggccgtatag ccgggtcgtg ctcgtgccgc cttgcgacgc
     1021 cgttgccgca gcgctcgcct cagccgcgta gaactcagca aactccttgc gttccttgct
     1081 gccgaaggtc caggcgccgc ggcccttctt tgcctcctcc tcgcggagct cgtagccgga
     1141 caggcgagga tagacgtagg accaccaggc gccgaaatcc atcgcattct gcatcgcctt
     1201 gccaaactcg ccatcattga cgatgtcgat tcccgatcgg acttgttgcc gaaccacgtc
     1261 ggcaactgag gatcgcagac gaggtaatag attttggtct ggcgcatcgt tcctataaag
     1321 ctcgacgagg tcgttcggac ggggaaggct gccaacgtgc gtcgtcagga tccggtcagt
     1381 gctccgtttc attgtcagtt cctaagaggc gtcatcccca aagttctttg gttgcaagcg
     1441 cggcaccttc ggtgagggcc tgaagcttcg cccatgcgat ttgcggatgg atgcgcccac
     1501 ccagaccgca gtcggtcccg gcaatcacgt tctcgcggcc gacgaccttg gcatagcgca
     1561 cgatgcggtc cgcgaccact tgcggatgtt caacgatgtt agtggcgtgg ctgacgacgc
     1621 ccggaatcag gagcttcccg tccggtaatt tgacgtcctg ccatacccgc cattcgtgtt
     1681 cgtgtcggac attcccggct tcgaccgaat aggcgccggc attgacgctg agtaccaggt
     1741 cgacgatgtc ctttaacgga atgtcggtgg tatgggggcc atgccagctg ccccagcaga
     1801 tgtggaagcg gatgcggtcg gccgaaagcc cgcgcagcgc atgattgagc gcttccatgc
     1861 gcacctgctc gaattttttg aattcccgca acggcggctc gggattgatc atatcccagt
     1921 tgtcggggag gctgggatcg tcaatctgca acacgacacc ggcatcgacg atcgctttgt
     1981 attcctcatg caaggcatcg gctgaggcga acaagaattc ctcttccgtc ttgtagtaaa
     2041 gatcgacacc tcgggcgaaa ctgcccggtc cgatcgagca catgaaacct tcttccgtgc
     2101 cggtcgcggc taacgctttt ttcagattcg cgatatcggc ttgcagcgcg gcgtggccga
     2161 tatatttgat cggtccggtg caggcggggc gcccgaccgg gcgccgcgcc gcgctgccca
     2221 tgaggctgcc gctcgactcc ggatcctgat agaactcgtt aaatctttgc cagtcgcggc
     2281 gattggcgaa cgaggtcagc gcgagatcgg cgacgctcga ctttttgtgc tgggtttcgt
     2341 gtgctgcggc ggccggactt ccaggcggcg aaaaccccga catgcgcgcg aaggcatagt
     2401 tccaccacgc accgtaatcg tacgagccgt ctaccggttt accgaactca ccgtcgtcgg
     2461 gaatatcgac gccgagttcg tgctgcttct tgaccacgtc ggcgacagca ctggcgagaa
     2521 cctgcgcgta tgcggcgtcg tcgcccttgg tctccccagc cgcgatcgac ttgctcaagg
     2581 cgataagatt ggccgggcgc gacaggctcc ccgcgtgagt agtcaaaatc cggtctttac
     2641 tgtgccgcat ctcgtctcct cccccgctct cataccgaac ggtcgcttcg caccaatctc
     2701 attggtaact gtcgacattg cgcgctggtc aaaccacgcg tgtccacact atgctgatga
     2761 taattccacg atacgacagg gaggtccaac atgacggata ctcggttaag ccggcgcacg
     2821 atgcttgagg gggcgaccgc gctgggcgtt ctggctgtca catcgggtga aactttcggc
     2881 cagcagacgg tctcgcgatc tgccgcggca ccggacactc cgctgccgcc gcgccgcgaa
     2941 ttcgtcatcc gcggcgccag cgtgctgacc atggatccca atattggcga tttcgcaacc
     3001 ggcgacgtgc atgtgcgcga tggcgcgatc gtcgcagtgg ccccacggat cgatgtggcg
     3061 aacgctcaga tcatcgaggc ccgcgatatg atctgcatgc cgggtttcgt cgacacacac
     3121 tggcaccttt ggacgagcct gctgcggcca tttatcaggg ccgatgtcga cgagctggga
     3181 tacttccccg tgagcttccg cttagggcag ctgttcacgc ccgaggacag ctaccgcagc
     3241 gtcttgctcg gcctcgccga agcattgagc gccggtgtga cgacggtgta caactgggcg
     3301 cacaacgtca ggagtccgga tcacgccgac gccgagctgt ccgccatgcg cgacgcaggg
     3361 attcgtggac gctttgccta cggtcccgcg cagggaatgc ctgataatca gccgatggac
     3421 atgactggcc tggcccgcgt gaaacgcgac tggatgccgg gaaacggtct gctcacgctc
     3481 ggcatctgct cgcgcaacat tggcgcaatg agcatcgggg gcgcgacgcg cgggacgctc
     3541 acggttgaaa tggcgaagcg ggactggggt ggagcacgtg ccctgggact ccccatcacg
     3601 ctgcacacct ccggcgcaag cccgatcaaa ctgctcgagg aggccgggct acttggctcc
     3661 gacgtacagc tcgtgcatcc gttgctcact acgcccgaag agcgtgcgat tctgaaggcg
     3721 cgtggcgtga gctatgcgac agcccccgtg ggcgaggcgc gccgtccgtc cagcgtcggt
     3781 gtgatccagc tcggcgagtt gttggaggcg ggcgtcaagg tcagtctatc gaccgaccac
     3841 accaccaact acaactgcga tcccttcgtc gctatgcgca ttctgttcgc gctacaccag
     3901 caccggatcg gcaac
//
LOCUS       DATLJT010000991         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2824287,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000991 DATLJT010000000
VERSION     DATLJT010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824287"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VM910_22845"
     CDS             <1..382
                     /locus_tag="VM910_22845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65393.1"
                     /translation="DYFCTISGSAAQPLQNDLIKGIAVFTRERLPSLPNVPTAHEQGI
                     DFDASTWFGFFVPKDTPAAIIKKLHDASVAAMESRSVQQQLATNGTYIVSPGKRSTEY
                     FERIIVPEIEKNAAPIKAAGMSVD"
     gene            753..1758
                     /locus_tag="VM910_22850"
                     /pseudo
     CDS             753..1758
                     /locus_tag="VM910_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1963..2946)
                     /locus_tag="VM910_22855"
     CDS             complement(1963..2946)
                     /locus_tag="VM910_22855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65394.1"
                     /translation="MFTRRRLIALSVGSALSLSGRAAWVQTQSLDWPNRVVRFIVPFA
                     AGGPTDIVARVVTEQLSNIWGQQAVIENRGGAGTNIGNEMVARSDPDGYTVLFATASL
                     AVNRSLYRSLSYDPIADFAAVSLVSRFSLFMFVPNSLPTKSVMEFVAYAKAHPGKLTL
                     ASPGTGSTPHLAGELLKQMAGIEMTHVPYRGASPALNDLIPGRVDCYFGSGALLENMR
                     SGQLRGLAVTGAKRDPVAPELPTMAEAGVPGYEVSSWHGLFVPARTPPEIVRKISADT
                     IAALAGPVVKSKLEQAGYMVVGSSPDELQTLLKSEIDKWSVVIKTVGIKIN"
     gene            3272..3568
                     /locus_tag="VM910_22860"
     CDS             3272..3568
                     /locus_tag="VM910_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65395.1"
                     /translation="MRRREFITLLGGAVATWPLAAVAQSTTQPIQATTANSPRIEVVR
                     VKPIDPAAPDLRFQLSDGSVDLMKSFTLDGFDGVGGAWKIPPTDETFDARYGRW"
BASE COUNT          753 a         1055 c         1073 g          691 t
ORIGIN      
        1 cgactatttc tgcacgatca gcgggtcggc ggcgcagccg cttcagaacg atctgatcaa
       61 gggcatcgca gtgttcacgc gcgaacgatt gccatcgctg ccgaatgtgc cgaccgccca
      121 tgagcagggc attgatttcg atgcgtcgac ctggtttggc ttcttcgtac ccaaggacac
      181 cccagccgcg ataatcaaga aacttcacga tgcaagcgtg gccgcgatgg aatcgcgctc
      241 ggtgcagcag caactcgcca ccaacggaac gtatattgtg tcgcccggaa agcgatcaac
      301 ggaatatttc gaacgcatca tcgtgcctga gattgagaaa aacgcagcac cgatcaaggc
      361 cgcaggaatg tcagtcgatt aagcgtagga cgctcggcct ggatgtgccg ccaacgctgc
      421 tggcccgcgc tgatgaggtg atcgagtaaa tgattgtgat ccgcgctcgt catttgctgc
      481 ggtgcatgag tctggtcctg gcgcaacgcg tcgttccatg cggcgtgcca attcggcagc
      541 aattggaggc gaagggggac atgccgaggt cgttgagagc atgtcgatcc gacgaaaatg
      601 accgagacat gcctctgccg cgcttagtgg gccatcgcgg acgtggtggt tccactactc
      661 gtttcgtgcg cgccgaattg ggaggtaata tccgtccggc gggaccgccg ccttgcaagc
      721 aatcattaga attaggtcct gggaggacat cgatgaccga actcactcgc cgtactgtac
      781 tcactggcgc cgtcgccact ggcgccgcga cctgcgctga taccactaac cagccacgtg
      841 ccggcccatg ctgccgcctc tgcagccggc gagcaggcgc ctggcttcta ccgctacaaa
      901 attggcagct ttgaatgcac ctcgatcaac gacggtgcgc gttcgttcac gatgcccgat
      961 accttcgtgc gcaatgtacc gaaggccgaa gcgctcgccg cagctgaagc ggcgtatatg
     1021 ccaaagggca tggtaacggt gccgttcaat ccgcaggtca tcaacaccgg ctctaaactc
     1081 gtgctgatcg actgcggctg gggagtcggc atgttcgagc cgagcaaagg cgcgatcggc
     1141 cgcacgctta gcaatctcgt cgccgccggc atcgatccga agagcatcga cgtggtgctg
     1201 atgtcgcacc tgcaccccga tcatacgaat ggcatccgcg ccgccgacgg ctcgatggca
     1261 ttccccaatg cggggatcac ggtgccggcg aacgattggg cgttctggat gagcgaggag
     1321 aatgcggcga aagcgcaatc aaatgaattg atgaaaggct atttcgccaa tgtgcggaag
     1381 gtttattccg gcatcgaagg caacgtgacc aaatacgaat ggggcaagga ggtggcatcc
     1441 ggcatcaccg cgatcgacac ccacggccat acgccgggac acacgtcctt tgtcgtgtcc
     1501 tccggctcgt cgaaggtgct gatccaatct gatgtcacca acattccgga actcttcctg
     1561 cgcaaccctg attggcacgt gatgtatgac atcgacccgg tggaggcggc caaaacccgc
     1621 cacaagttct acgatatggt ctcggccgag aaggcactgg tcgtcggctt ccacttcaac
     1681 tttccgtcgc tgggttatgt ggaaaaggac agcacgaaat atcgcctagt gccgatcgct
     1741 tggaatccgg tgatctgatc gtcgccatcg cgagcagcaa gggagagccg cccgctcgag
     1801 ggcggctttc gcatgagggg ggtactccgt ggcggccgaa gtgcgccgga ataccgactt
     1861 atcaatggcc agaaattgca gtgaagccgc gaacaccgcg tgttgttcga ccggacgttc
     1921 ggccgaactt gtcgaatttg aaggcggcgc agagtactcg ccctaattga tcttgatccc
     1981 gaccgtcttg atgacgacgc tccatttatc gatttccgat ttgaggagag tttgaagctc
     2041 atcgggcgaa gagcccacca ccatgtaacc ggcttgttcc agcttgctct tgaccacagg
     2101 accggcgaga gctgcgatgg tatcggcact tattttcctg acgatttcag gcggcgttct
     2161 ggccggcacg aacaagccgt gccacgacga cacctcgtaa cccggaacgc cagcctcggc
     2221 catcgtgggc aattccggcg ccaccggatc tcttttggcg ccggtaaccg caagtccacg
     2281 cagctggccc gaccgcatgt tttcgagcag cgcgccgctg ccgaaataac agtccacgcg
     2341 gccggggatg aggtcgttga gcgcgggcga cgcgccacga tagggcacat gcgtcatctc
     2401 gatcccggcc atttgcttga gcagctcgcc ggccaaatgc ggcgtactgc cggtgccggg
     2461 ggatgctagt gtgagcttgc ccgggtgcgc cttcgcatag gccacgaact ccatgaccga
     2521 tttggtcggt aacgagttcg gcacgaacat gaacaatgaa aacctggaaa ctagggagac
     2581 tgccgcaaaa tccgcaatcg ggtcgtagct gagcgagcgg taaagactgc ggttcactgc
     2641 cagggatgcg gtggcaaaca atactgtgta gccatcggga tcggaccggg cgaccatctc
     2701 attgccgata ttggtgccgg cgcctcccct gttctcgatg acggcttgct ggccccagat
     2761 gtttgagagc tgctcggtga cgacgcgggc gacaatgtcc gtcggtcctc ccgcggcaaa
     2821 cggcacaatg aagcgcacga ctcgattggg ccaatccagc gattgagtct gcacccacgc
     2881 ggcacgaccg ctaaggctga gtgcggaccc gacggacaga gcaatcaggc ggcggcgcgt
     2941 gaacacgagt accccctggc tacatttatt ctatccgagc cgagaacact tgcgcagagc
     3001 caccggcagt agtcatagcc gggattgaac tcccgccaat tatctcgttg agctttgcgg
     3061 cgcgccggca aattaggggt gtcctcatca atgcgcagat cggaggtccg atttcagggc
     3121 aatgcggaaa tagaccgacg ctcgccctga tcgcatttga cgcgtctgac ccatctctgc
     3181 cattcgacaa tcaattttgc tgtgatgcac agcagcggct ctcgcgcaac ggtgtggtaa
     3241 tatgcagtcc ccaacctgag gaggggagcc catgagacgg cgcgagttca tcacgctact
     3301 cggcggcgca gtggcaacgt ggccgctcgc cgcagttgcg cagtcgacga cgcaaccaat
     3361 tcaagcgacg acagcgaact cgccgcgaat cgaggtggtg cgcgtgaagc ctatcgaccc
     3421 ggccgcccca gaccttcggt ttcaattaag cgatgggtcg gttgacctga tgaagtcctt
     3481 tacccttgac ggcttcgatg gcgttggtgg cgcctggaag ataccgccta ccgacgagac
     3541 cttcgacgcg cgctacggtc gatggtgaga cc
//
LOCUS       DATLJT010000992         1977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2134000,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000992 DATLJT010000000
VERSION     DATLJT010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134000"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..691
                     /locus_tag="VM910_22865"
                     /pseudo
     CDS             17..691
                     /locus_tag="VM910_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1444..>1977)
                     /locus_tag="VM910_22870"
     CDS             complement(1444..>1977)
                     /locus_tag="VM910_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HVI65396.1"
                     /translation="GADAIMLSAESAAGKYPVEAVSTMNRIGEEVERDPSYRAVLTEQ
                     RPEPEATAGDAIADAARVIAETLDLPALICWTSSGSTAIRVARERPKPPIVAITPNIA
                     TGRKLSVVWGVHCVVAEDAHDQDDMVNRAGSIAFRDGFVRAGQRVIIVAGVPLGVPGT
                     TNMVRIASVGPDGEANM"
BASE COUNT          324 a          646 c          664 g          343 t
ORIGIN      
        1 cgggacagga aacgccatgg tttatcggcg gacgcaccag gtcgtgaagc gcctcgcggc
       61 gcgccgcagt gccattctgg cagccgcacg tgatgccgca gccgaatccg gcatggccgc
      121 ggtccagatt gcgccggtcg ccgtgcgcgc caatgtcgcc gccggcacgg tctaacgcta
      181 ttttccctcc aaggctgacc tgatctccga actgatcgcc gatgtctcgc gcgatgaact
      241 cgccgcgatc cgccgcgcgg ccgatgccgc gcccgggcca tcttccgcgc tggctgcggc
      301 ggtttccacc gtcgccgtcc acgtgctgtc ccagcgcaag ctttcctggg gcattctggc
      361 cgagccggtt gacgtcgatg tcaccgcatc gcggctcgcc agccgccgcg agatcgcggg
      421 cgaaatcgct agccgcatcg atgccgcagt ccgcgccggg catctgccgg cacaggacac
      481 ccagcttgcc gcgaccgcgc ttctcggtgc gctgcacgag gcgctggtcg ggccgctcgc
      541 gcccgacaat ctcgacgatc ccgccaggat gcgcgacgcc gttcagagcg tgaccctgct
      601 tgcgctgcgc gcggtcgggg tgatggatgc acgcgcccgc gggctcgtgg tgcaggccgc
      661 tctgccggca aagacgctgg tcggagcgta gcggcgtcgc cgggccgatg ccgttttcgc
      721 agaggcgcgc ggtgaagcca gttcgcttgc caggcaaacc agtcggcgcg atttgaggcg
      781 gatcgcactt cgatttcgct ccaaaagttt gcaaaagcgg gtgttcggaa aatcgaaatt
      841 cgcgagccga tgcaatgtgc tgcgccatcc aatccttgag ctgaaaaatt ttcactttcc
      901 gaaaatcaga aaacgtgcgt atcctggcgc tgtcccgcac tcgcaagagg ggcgtacgcg
      961 tcgtcacgaa cgttgagcgc gggatgcgat ggacgcgggc tgtgccgcgc gacgagcggt
     1021 acaggcgcgg acggcgaagg cgtgtggtcc tggcacccct gggctggtgc caagtctggc
     1081 gggcgatgac ctgccgggtg acggtgacta aaaagtcatg gacaccgggg agagcacgca
     1141 cgaagccgtt aaaaccattc gcgcagggag ggccggcatg ttccggtcga acctgtggtg
     1201 actacgctcg tgtggttctt tcattcccac gcgaggctac gggtgcagcc agcatccggc
     1261 tttccctgcg ccctctgttt tcgagggaca cagaccccgt atcactcggg cgcatcgcgc
     1321 gggccgggat cgcggagacg tgtcggctgt ttgaaaaacg aatcggaaaa cctgcaaacg
     1381 tcattgcgtt gcgcgaaagc gacgacccgc caccgcgcga agcgcggacc agcatcggcg
     1441 tcgttacatg ttggcttcgc catctggtcc gacggaggcg atgcgcacca tgttggtcgt
     1501 gccgggaacc ccgagcggga cgccggccac gatgatcacg cgctggccgg cgcggacgaa
     1561 gccatcgcgg aaagcaatgc tgccggcgcg gttgaccatg tcgtcctggt cgtgtgcatc
     1621 ttcagcgacc acgcaatgca cgccccagac caccgacagt ttgcggcccg tggcgatgtt
     1681 cggcgtgatg gccacgatcg gcggttttgg acgctcgcgc gccacccgga tcgcggtcga
     1741 gccggacgag gtccagcaga tcagtgcggg caggtcgagg gtctcggcga tcacgcgcgc
     1801 agcgtcggcg atggcatcgc ctgcggtcgc ttccggctcg ggccgttgtt cggtgagcac
     1861 ggcgcgatag ctcggatcgc gctccacttc ctcgccgatg cggttcatgg tggaaaccgc
     1921 ttccaccgga tatttgcccg ccgccgattc cgccgacagc atgatcgcgt cggcgcc
//
LOCUS       DATLJT010000993         4008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3053754,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000993 DATLJT010000000
VERSION     DATLJT010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053754"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VM910_22875"
     CDS             <1..501
                     /locus_tag="VM910_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI65397.1"
                     /translation="LEQTQVGMAGQQTAIGDTIGLAIKTLEKSTAKQKLLILLTDGND
                     TASRVPPEHAADIAHQNGVVVYTIGVGDPAASGENRVDLSVLRNVASTTQGQFFRAED
                     GVQLEAIYADIDKLAPAKLDTLSWRPKLPLFQWPLGAAVILALALWLALLAGSEWGRR
                     KVARHA"
     gene            494..2026
                     /locus_tag="VM910_22880"
     CDS             494..2026
                     /locus_tag="VM910_22880"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI65398.1"
                     /translation="MHDAAAIPFHLLRPLWLAALVPVAIVLALVLWRQSPEAQWGGVI
                     ARHLLKNLIVQPGRRWSVNPAYLVATGLVLAIVGLSGPTWKRELPPFVEDKAPLMIAL
                     ALSSSMGETDVAPSRLERAKQKIRDLLAARTGARTGLIAYAGTAHLVMPLTDDRTVIA
                     PFLAALLPGLMPQDGKNAAAAIALATQSLATEPVAGTILLVADDLGSADGGALRQSAG
                     RNNLLMLAVRPSQGAETATSLARSDVVPVSVDDADIRALERRVETRFQAAQGDAFGAQ
                     WQDEGYWLLLPAALIALFWFRRGTTVPWVIVFFLAFHAWPANAQDASWFKSLWLTPDQ
                     QGRIAFDRGDYAAAAKLFGDPMWRGIASYRAYDFLEAARQFQEVDTTQGKFALGNAQA
                     QNHAYEKAIKAYDEVLKVEPQNVAAKTNRAIVAAALKAQEEKRRKQEQDDSAPPDEKA
                     DETRVDPDQKGGKKIQVTPDDLTTEGAAEAWMREVQTTPADFLKLKFAIQAAAPPAGT
                     KP"
     gene            2023..3411
                     /locus_tag="VM910_22885"
     CDS             2023..3411
                     /locus_tag="VM910_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HVI65399.1"
                     /translation="MTRWSLALILVALALSGARAQTAAPEPTLRTAIDPPRVVVGQKT
                     TLRVEVLAPNYMTAPPELPGFQVRNAVTRQLQSVNINDQQGGTTYAGVRFEFAIYPQE
                     PGTYAVGGQKVTVHYAAEPPATRDAELALPRMEFAALIPDGAAALRPFVAATKLTVEQ
                     TVQRSSEQLKSGDAVTRTVTIKADGTPAMLLPPQSFAAVDGLALYPAQPSLEDHTDGR
                     TDALTSTRVDSATYMLQRPGDYVLPAIDGSWWNIGSGRIERVHLDAITLQVAANPNLP
                     VAATANQRARRDWDGIIDFMWDHWLLAAIAVIALGAAAWFAPRMARAMAARYRQRREA
                     YLRSEAWSFEQFRSATRGGDVKAAYFALLDWLQRFAPAAPDHNLESLKAGARDAALDQ
                     EIGSIERQLFAPDRNTGNWSLAQLLRRVSAARMTLQRQAAQTETARPLPQRLNPVSDL
                     AAPDRRFRLPAR"
     gene            3486..>4008
                     /locus_tag="VM910_22890"
     CDS             3486..>4008
                     /locus_tag="VM910_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVI65400.1"
                     /translation="MSSEAKNNAMVTGSGKDDAQAIDRRNVLLGASSLVAATALTSEA
                     LAQAQKAAPSAAPTAAPAAGSKPNILFIMGDDIGWFNVSAYNMGVMGYRTPNIDRIGR
                     EGAVFTDWYGQQSCTAGRAAFITGQTPIRTGLTKVGLPGAELGLGPLDPSVADVLKTY
                     GYATGQFGKNHLGD"
BASE COUNT          738 a         1351 c         1310 g          609 t
ORIGIN      
        1 ctggagcaga cgcaggtggg catggcgggc cagcagaccg cgatcggcga caccatcggg
       61 cttgcgatca agacgctgga gaagagcacg gcgaagcaga agctgctcat tcttctcacc
      121 gacggcaacg acaccgcaag ccgcgttccg cccgaacatg ccgccgacat cgcgcaccag
      181 aacggcgtcg tcgtctacac gatcggcgtc ggcgatcccg cggcttccgg cgagaaccgg
      241 gtcgacctca gcgtgctgcg gaacgtcgcc agcacgacgc aggggcagtt cttccgtgcc
      301 gaggatggcg tacaactcga ggcgatctac gccgacattg acaagctcgc gcccgccaag
      361 ctcgacacgc tgtcatggcg gccgaagctg ccgctgttcc agtggccgct cggcgccgcc
      421 gtgatccttg cgctggcgct gtggctcgcg ctgctcgcag ggagcgaatg gggccgcagg
      481 aaggtggcgc gccatgcatg acgccgccgc cattccgttc cacctgttgc ggccgctgtg
      541 gctcgcagca cttgtgccgg tcgcaatcgt gctcgcactg gttttgtggc gacaaagccc
      601 ggaagcgcaa tggggcggcg tgatcgccag gcatctcctg aagaatctca tcgtgcagcc
      661 cggccggcga tggagcgtca atcccgccta tctcgtcgcg acgggccttg tgctcgccat
      721 cgtcggattg tcgggaccca cctggaagcg cgagctgccg ccctttgtcg aggacaaggc
      781 gccgctgatg atcgcgctcg cgctgtcgtc ctcgatgggc gagaccgacg tggcgccgtc
      841 gcggctggaa cgcgccaagc aaaagatacg ggacctgctt gcggcccgca cgggcgcgcg
      901 aaccgggctg atcgcctatg ccggcacggc acatctggtg atgccgctca ccgacgatcg
      961 cacggtcatc gcgccgttcc ttgccgccct tttgcccggc ctgatgccgc aggacggcaa
     1021 gaacgccgcg gcggcgattg cacttgcaac acaatcgctg gcgacggagc cggttgccgg
     1081 aacaatactg ctcgtcgccg acgatctcgg cagcgcggac ggcggcgcgc ttcggcaatc
     1141 agccgggcga aacaatctgc tgatgctggc cgtgcgtccc tcgcaaggcg cggagacggc
     1201 aacctccctt gcacgatccg atgtcgtacc tgtgagcgtc gacgatgccg acatccgcgc
     1261 gctcgagcgc cgcgtcgaga cccgcttcca ggccgcacag ggcgatgcct tcggcgcgca
     1321 atggcaggac gagggctact ggctgctgct gccggccgcc ctgatcgccc tgttctggtt
     1381 caggcgcggc accaccgtgc cgtgggtgat tgtgtttttc cttgccttcc atgcgtggcc
     1441 tgcgaacgca caggacgcct cgtggttcaa aagcctctgg ctgacgccgg accagcaagg
     1501 acggatcgcc ttcgaccgtg gcgactatgc cgctgcagcg aaactgtttg gcgatccgat
     1561 gtggcgcggc attgcttcct accgcgccta cgacttcctc gaggcagcca ggcaattcca
     1621 ggaggtcgat acgacgcaag gaaaattcgc gctcggcaac gcgcaggcgc agaaccacgc
     1681 ctatgaaaag gcgatcaagg cctatgacga ggtgctgaag gtcgagccgc agaatgtcgc
     1741 ggccaagacc aaccgcgcga tcgtcgccgc cgcgctgaag gcgcaggagg aaaagcgccg
     1801 caagcaggag caggacgatt ccgcgcctcc ggacgagaag gccgacgaga cgcgcgtcga
     1861 tcccgatcag aaaggcggca aaaagattca ggtgacgccc gacgacctca cgaccgaagg
     1921 cgcggcggaa gcctggatgc gcgaggttca gaccacgccg gcggatttcc tcaaactgaa
     1981 attcgcgatc caggcggcgg cgccgccagc ggggacgaag ccatgacgcg ctggtccctc
     2041 gcattgatcc ttgtcgcgct ggcgctgtcc ggcgcgcgcg cgcagactgc ggcgccggag
     2101 ccgaccttac ggacggcgat cgatccgccg cgtgtcgtgg tcgggcagaa gaccacgttg
     2161 cgggtcgagg tgcttgcacc gaactacatg accgcgccgc cggagctgcc gggattccag
     2221 gttcgcaacg cggtgacgcg gcaattgcag agcgtcaaca tcaacgatca acagggcggc
     2281 acgacctatg ccggcgtgcg cttcgaattc gcgatctatc cgcaggaacc gggcacctac
     2341 gccgtcggcg gccagaaagt caccgtccac tacgcagccg agccgccggc gacacgcgat
     2401 gccgagctcg ccttgccgcg tatggaattt gcagccctca tcccggatgg cgcggccgcg
     2461 ctacgcccgt tcgtggccgc gaccaagctg acggtcgaac agacggtgca gcgctcctcg
     2521 gagcagctca agtccggcga cgccgtgacg cggacggtga cgatcaaggc ggacggcacg
     2581 cccgcgatgc tgctgccgcc acagagtttc gcagccgtcg atggcctcgc gctctatccg
     2641 gcacagcctt cgctcgagga ccacacggac gggcggaccg acgcgctgac atcgacgcgg
     2701 gtcgactccg cgacctacat gctgcaacgc cccggcgact atgtgctgcc cgcgatcgac
     2761 gggagctggt ggaacatcgg cagcggaagg atcgagcgcg tgcatctcga cgcaattacg
     2821 ctgcaggtcg ccgcaaatcc gaatctgccg gtcgccgcta cggccaacca gcgcgcacgc
     2881 cgggattggg acggcatcat cgatttcatg tgggatcact ggctccttgc cgccatcgcc
     2941 gtgattgcgc tcggcgcagc ggcttggttt gcgccgcgca tggcgagagc gatggccgcg
     3001 cgataccggc agcgccgcga ggcctatctc cgatcggaag cctggtcgtt cgaacaattc
     3061 cgcagcgcga cccgcggtgg cgacgtcaag gccgcgtatt tcgcgctgct cgattggctg
     3121 cagcgctttg caccggccgc gcccgatcac aacctcgaaa gcctgaaggc cggcgcgcgc
     3181 gacgcagcgc tcgatcagga gatcggatcg atcgaacggc aattgttcgc gcccgatcgc
     3241 aacacgggca attggtccct ggcccaattg ctgcggcgtg tcagcgcggc gagaatgacg
     3301 ttgcagcggc aagcggcgca gaccgagacg gcccggccgc tgccacagcg gctcaaccct
     3361 gtcagcgacc tcgccgcgcc cgatcgccgg ttccgtctgc cggcacgatg aaaaaggaga
     3421 ttcaaaagac gcagaaggtt tccatcatga ccgtccgcaa accttacaac aaaagggagt
     3481 gtcgaatgag cagcgaagcg aaaaacaacg cgatggttac tggttcggga aaagatgatg
     3541 cgcaggccat cgatcgccgt aacgtgctcc tgggcgcgtc gtcgctggtc gccgcaaccg
     3601 ccctgacctc ggaagcgctg gcgcaggcgc agaaggcagc accctcggcc gcaccgacgg
     3661 ccgcgcccgc cgccggcagc aagccgaaca tcctgttcat catgggcgac gacatcggct
     3721 ggttcaatgt cagcgcctac aacatggggg tcatgggcta tcgcacgccc aacatcgacc
     3781 ggatcggaag agagggcgcg gtcttcaccg actggtacgg ccagcagagc tgcaccgcgg
     3841 gccgcgccgc cttcatcacc ggtcagacgc cgatccgcac cggcctcacc aaggttgggc
     3901 tacccggcgc cgagctcggg ctcgggccgc tcgatcccag cgtcgccgac gtgctcaaga
     3961 cctacggcta tgccaccggt cagttcggca agaaccatct cggcgacc
//
LOCUS       DATLJT010000994         2954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3743347,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000994 DATLJT010000000
VERSION     DATLJT010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743347"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VM910_22895"
     CDS             <1..327
                     /locus_tag="VM910_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65401.1"
                     /translation="NRRVVSHAVPMVGYLKGAPLRSPLDLSFAFASEQTIDELAFMAK
                     IDPLEFRRNNIGDARWLGVLNAAAGSRWARITCLMALRSPISRSIGAPATSSPPTFTP
                     RSTRGR"
     gene            228..656
                     /locus_tag="VM910_22900"
     CDS             228..656
                     /locus_tag="VM910_22900"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65402.1"
                     /translation="MSYGAAVADIEVDRRTGNIVATHVYAALDAGQVINPALVENQIV
                     GQAIQATSRVLKEEVTYDKNGVTSLDWVSYPVLRFAEHPQVTAVVVQRLDEPSTGAGE
                     EVMGATAAAIANAFFDATGVRLRQYPMTPERVKAALAAKT"
     gene            complement(843..1835)
                     /locus_tag="VM910_22905"
     CDS             complement(843..1835)
                     /locus_tag="VM910_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI65403.1"
                     /translation="MTELTRRRLVTGAIAASAATTLMPLQATAPAHAAAPVAGKQAPR
                     FYRYKVGSFECTSINDGARTFPMPDGFVKNVSKDEALAAAEAAYMPKGMVTVPFNPQI
                     INTGSKLVLIDTGYGPGIGPTVGLLPAHMAAAGIDPKAIDVVILSHLHPDHINGVRTA
                     DGTPAFPNAEMMVPTQDWAFWMSEDNFNKAQSNPMMKAYFENVRKVLSPFASKVTKYD
                     WGKEVAPGITAIDAHGHTPGHTSFALASGSSKVLIQSDVTNIPELFLRNPDWHVMFDV
                     DPVQAAQTRHKFYDMAAAEKALVVGFHFPFPSLGYVEKDGGKYRLVPVAWNPVI"
     gene            complement(2678..>2954)
                     /locus_tag="VM910_22910"
     CDS             complement(2678..>2954)
                     /locus_tag="VM910_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65404.1"
                     /translation="LGKIVEYAEKKRLFLNPLHPYTEALLAAVPVPNPKLRRAKRPLA
                     GDVPSPVEPPPGCPFHTRCGYAQERCRREIPQLREVAPGHWVSCHFR"
BASE COUNT          559 a          893 c          941 g          561 t
ORIGIN      
        1 aaccgccgcg tggtcagcca cgccgtgccc atggtcggct atctcaaggg cgcgccgctg
       61 cgctcgccgc tcgatctgtc gttcgcgttc gcctcggagc agacgatcga cgagctcgcg
      121 ttcatggcca agatcgatcc gctcgaattc cgccgcaaca atatcggcga cgcgcgctgg
      181 ctcggcgtgc tcaacgcggc cgcgggatcg cgctgggcac gcatcacgtg tcttatggcg
      241 ctgcggtcgc cgatatcgag gtcgatcggc gcaccggcaa catcgtcgcc acccacgttt
      301 acgccgcgct cgacgcgggg caggtgatca atcccgccct ggtcgagaac cagatcgtcg
      361 ggcaagcgat ccaggcgacg agccgcgtgc tcaaggagga ggtgacgtac gacaagaacg
      421 gcgtcaccag cctcgattgg gtgtcgtatc cggtgctgcg cttcgccgag catccgcagg
      481 tgacggcggt cgtggtgcag cgcctcgacg aaccttcgac cggcgccggc gaggaggtca
      541 tgggcgcgac cgcggccgcg atcgccaacg cgttcttcga cgcgaccggg gtgcggctgc
      601 gccaatatcc gatgacgccc gagcgcgtga aggccgcgct cgccgccaaa acctaggcgt
      661 ataagatata agcgtaaggg atgagcgtcg tcgcgtggcg cgcctttaag gcgcgctagg
      721 ctgtgtactc ctaaaccggt ccgatggatg ccgcgcggat ctccttcagc gccacgccca
      781 atagcggcag cactgcaaca atgcttcgct gtgcagtgct gccgattgca aaacgagcag
      841 aatcagatca ccgggttcca ggcgaccggc accaagcgat actttccgcc gtccttctcc
      901 acatagccga gcgacgggaa tgggaagtgg aatccgacca ccagcgcctt ttcggccgcg
      961 gccatgtcgt aaaacttgtg ccgggtctgc gccgcctgca cgggatcgac gtcgaacatg
     1021 acgtgccagt ccggattgcg caggaacagc tccggaatgt tggttacgtc ggactggatc
     1081 aacaccttgg acgagccgga tgcgagggca aacgacgtgt ggcccggcgt gtgcccgtgc
     1141 gcgtcgatcg cggtgatgcc tggcgccacc tccttgcccc agtcgtactt ggtcaccttg
     1201 ctggcgaacg ggctcaatac tttgcgcacg ttctcgaaat aggccttcat catcggattg
     1261 gactgcgcct tgttgaaatt gtcctcgctc atccagaacg cccaatcctg cgtcggcacc
     1321 atcatctccg cattcggaaa cgcgggcgtg ccatcggccg tgcgcacgcc gttgatgtgg
     1381 tccggatgga ggtgagagag gatcacgaca tcgatggcct tcggatcgat gccggcggcc
     1441 gccatgtgag ccggcagcag cccgacggtc gggccaatgc cgggcccgta cccggtatcg
     1501 atcagcacga gcttggatcc ggtgttgatg atttgcggat tgaacggcac cgtgaccatg
     1561 cccttgggca tgtaagcggc ttcagcggcg gcgagtgcct cgtccttgct gacgttcttc
     1621 acaaagccat cgggcattgg gaaggtgcgc gcgccgtcgt tgatcgacgt gcattcgaag
     1681 ctgcctactt tgtagcggta gaagcgaggc gcctgtttgc cggcgacggg tgcggcggca
     1741 tgcgccggcg ccgtagcttg caagggcata agcgtcgtgg cggccgaggc agcgatggcg
     1801 ccagtcacta atcggcggcg agtgagttcg gtcatggata tcctcccctc acagttgttc
     1861 tgtgaattat tgagcgagtg agcggcttgc cggcacagcc ggagagcaga tgataacacc
     1921 tcatgagttc gattaaaaca tacgtgcgtg cctcgagcgg cgtgcgcttg cgctttgctc
     1981 gcgggatcgc aatgcgaacc cgcctccatt cgcaggtaac ccaaacggtg tggtcgtata
     2041 tccgccgtag ttgatgggcg aacattgcgg atatgctgtc gcgtagctcg tggcacgaac
     2101 gcttgcgagg atatcctatg acgctggtag gagtgtcggc cttcacaatg atcgcaatag
     2161 tcgggctcgg ggttcaggcc gaagcgcaaa cgccgtgcca agaatacctg cgactgcgca
     2221 acgcggccaa tgaggcctgg aaacaggcga tgagcgtacc gccatcggag cgctgcggaa
     2281 tgctttatca tgcctccttg gccgcggagg cgacactcaa gtacgcaaat aacaatcgcc
     2341 agtcatgcga catttctgtc ccactgctga accaagtgga acggtatcac cgcgaggcgg
     2401 tacaggctcg ccacaatgct tgtgctggaa gccctcttcg accatatcca gcagatatca
     2461 tccaacgttg agatcataga agcttggtcc agtgcccgcg catttcggca attgacgtcc
     2521 gagttggggc attcgcgtcg gaccgaaacg cttccgaccg tcgcggcatg tccgctttgc
     2581 ccccgaaagc gacaaataac ggtcagtctc ggtatgtccg cttagtgcca aaagcggatc
     2641 acctcgtcgc cgccggttgg tgtggtatat gctggggtta gcgaaaatgg catgaaaccc
     2701 aatgccccgg tgcgacttcg cgcaattgcg ggatctcccg gcggcaacgc tcctgcgcat
     2761 acccgcagcg ggtgtggaac gggcagccgg gcggcggttc gactggactc ggtacgtcgc
     2821 cggcaagcgg gcgcttcgcc cgcctgagct tgggattggg aacaggcacc gcggcaagca
     2881 aggcctcggt atagggatgc agcggattga ggaagagcct ctttttctcc gcatactcga
     2941 cgatcttgcc gaga
//
LOCUS       DATLJT010000995         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2824586,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000995 DATLJT010000000
VERSION     DATLJT010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824586"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..440)
                     /locus_tag="VM910_22915"
     CDS             complement(78..440)
                     /locus_tag="VM910_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65405.1"
                     /translation="MLDRAVFRFSIDALVIILGAPSVRGLALPDAAEPSPSRASLNGL
                     DGINFFNAAILAGFGPYVAVYLAEQKWTQENIGFVLAASGLAGLLALIPGGELLGACG
                     VLPMSSIRHELLKRCPLT"
     gene            complement(445..1539)
                     /locus_tag="VM910_22920"
     CDS             complement(445..1539)
                     /locus_tag="VM910_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVI65406.1"
                     /translation="MKIAQVAPLMESVPPRLYGGTERVVSYLTDELVRLGHEVTLFAS
                     GDSVSTANLVRCVPTALRLDASVRDPIPYYMLMLDRVRELADEFDVLHFHIDQFHFPL
                     FRPIANKTVTTLHGRQDRPDLKPLYLGFSDMPLVSISNAQRKPILNANFVATVYHGLP
                     LHLLKPTCRVSGGYVAFLGRISPEKRPDRAIRIAQALELPIKIAAKVDKVDEAYFREK
                     IVPLLNQPGVQFIGEIDERAKSEFLGNAGALVFPIDWPEPFGLSMIEAMACGTPVLAF
                     CCGSVPEIIDPGVTGAIVRTMAEAIRTLPQVLALDRRTVRQGFEQRFSATRMAKDYIQ
                     VYGSVLKRPSLMERRVVVSRLEPKVEKEMS"
     gene            complement(1785..2189)
                     /locus_tag="VM910_22925"
     CDS             complement(1785..2189)
                     /locus_tag="VM910_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65407.1"
                     /translation="MPVEDAQGSTARIAGPRPPPSTVALAIFASHHRRIIIHYKKNDP
                     KFDPDQAFGSDCCCVLDRTTLGIFRTRLSEAASVSPSGPSARTISELNIARYRNLLKE
                     ETNASNRKIIVKLLAEEEAKRAKLSAQKDKDE"
     gene            2200..2877
                     /locus_tag="VM910_22930"
     CDS             2200..2877
                     /locus_tag="VM910_22930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI65408.1"
                     /translation="MLSDEERAQLATIASIVRFKKGAEIYRHGHRANVIFNIVSGVVK
                     AHKTDRDGREHITAFLFPDDLLGLSEEGRYTNSATAITPMTAFQIPVSALRGRLSKDA
                     SLEFHVICKLCQELRQAQRHAFLLAQRQALSRVAMFLQLLEQLQASRGESTTEIYLPM
                     SRSDIGEYIAMSLEAVSRAFRSLTARRIIKSRDRRHVKIIDRSAFEDLVDEMAHLRPR
                     NRPAGDK"
     gene            complement(3269..3397)
                     /locus_tag="VM910_22935"
     CDS             complement(3269..3397)
                     /locus_tag="VM910_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65409.1"
                     /translation="MRISASSGRSRDTVWSIAAVAADFLLNLIAYNLIRIPKLVAA"
BASE COUNT          756 a         1011 c         1011 g          747 t
ORIGIN      
        1 tcacttggct cagggaccga ttgggcatgg gttatgactg atgtgtactt tgagggcggc
       61 gaccatgcca atttggttca tgtcagtggg caacgtttca gaagctcatg gcggatgctc
      121 gacatgggca atacaccgca cgcacctagc agttcgccgc cgggtataag ggcaagcaag
      181 ccagcgagac cgctcgcagc caggacgaaa ccaatgttct cctgcgtcca tttctgctcg
      241 gccagataaa cggccacata cggaccgaaa ccagccaata tagcggcatt gaaaaaattg
      301 attccgtcaa ggccgttcag gctcgcacga gatggcgagg gttcagcagc atcgggcaac
      361 gccagaccgc ggacggatgg cgcgcccaag atgattacta gcgcgtcgat agagaaccgg
      421 aacactgctc gatcgagcat cccgtcagct catttctttt tccaccttcg gctccagcct
      481 cgacacgacg acccgccgct ccatgagcga tggccgcttg agcaccgaac cgtacacctg
      541 aatgtaatcc ttggccatgc gcgtggccga gaagcgctgc tcgaaccctt gccgaaccgt
      601 tcggcgatcg agcgcgagca cctggggcag cgttctgatt gcctcggcca tcgtgcggac
      661 gatcgcgcct gtcacgcctg gatcgatgat ctcagggaca gagccgcagc agaaggcgag
      721 caccggcgtc ccgcaggcca tggcctcgat catcgacaat ccgaacggct cgggccagtc
      781 gatcgggaat acgagcgctc ccgcattgcc caagaactcg ctcttggcac gttcatcaat
      841 ctccccaata aattgcacac ccggctgatt gagcagcggg acgatttttt cgcggaaata
      901 tgcttcgtcg actttgtcga ccttggctgc gatcttgatc ggcaattcga gcgcctgcgc
      961 gatccgtatc gcacggtcgg gccgcttctc cggagagatg cgtccgagga acgccacgta
     1021 gccgccgctt accctacagg tcggcttgag cagatggagc ggaaggccgt gatagaccgt
     1081 ggcgacaaag ttcgcgttga gaatcggctt gcgctgtgca ttggagatcg acaccagcgg
     1141 catgtcgctg aaacccaggt atagcggctt gagatcggga cgatcctgcc ggccatgcaa
     1201 agtggtcacg gttttatttg cgatcggccg aaatagtgga aaatgaaact gatcgatgtg
     1261 aaagtgcaga acgtcgaatt cgtccgcgag ctcgcgcacc cggtcaagca tcagcatata
     1321 gtagggaatt ggatcgcgta cactggcatc caggcgcagc gccgtgggca cgcagcgcac
     1381 caaattcgcg gtcgaaaccg aatccccact cgcaaacagg gtcacctcat gacccagccg
     1441 gactagttca tcggtgaggt aggagacaac gcgttcggtg ccgccataaa gccgcggcgg
     1501 cacgctctcc atcagcggcg cgacctgcgc tatcttcata tttttcctct ccgccgatgt
     1561 gaatggcacc gccgtattgc cctctccgtt tggctgattc cgttagctag ctgggccaat
     1621 gttggttttg tgtaatccat gtgtgatttt ctgagacttc ttcaacgcca cctgtcttgc
     1681 gcccagtcct cttcggtctt ccgcctggct gccgccgccg ccagggctta cgcactcgcg
     1741 taaatggcca aaccggaccg tcgaactgaa attcgtcgac gaaatcattc gtctttatcc
     1801 ttttgcgccg aaagctttgc ccgcttggcc tcttcttcgg ctagtaattt gacaatgatc
     1861 ttgcggttcg acgcgttcgt ctcctccttc agcagattcc ggtagcgcgc gatgttgagc
     1921 tcgcttatcg ttcgtgcaga agggccagag gggctcacgg aagcggcctc agacagtcgg
     1981 gttcggaaaa ttcctagcgt cgttcgatcg aggacgcagc aacaatccga accaaaagct
     2041 tgatcgggat caaactttgg atcgtttttt ttgtagtgta tgattattct gcggtgatgc
     2101 gaggcgaaga tggcgagggc gacagtggaa ggtggaggtc gcggccccgc gattcgcgcc
     2161 gtcgacccct gggcatcctc aaccggcaag atgcatcagt tgctcagcga cgaagagcgc
     2221 gctcaactgg caacgatcgc gtcgattgtt cgcttcaaga aaggtgcgga aatctaccgc
     2281 catggtcacc gcgccaacgt gatattcaac atcgtcagtg gggtggtaaa ggcacataag
     2341 acggaccgcg acggaagaga acacatcact gcgttcttgt tcccagatga tctgctgggg
     2401 ctgtccgaag aaggaagata tacaaattcg gctacggcga tcacaccgat gaccgcgttt
     2461 cagataccag tctccgctct acgcggcagg ctatcgaaag atgccagctt ggaatttcat
     2521 gtcatctgca agctctgcca ggaactccgt caggcgcagc gtcacgcgtt tctccttgcg
     2581 cagaggcaag ctctttccag ggtcgcgatg ttccttcagc tgctcgaaca gcttcaagcc
     2641 tccagagggg aaagcacgac cgagatatac ctcccgatga gccggtccga catcggcgag
     2701 tacatcgcaa tgtcgcttga ggcggtgagc agggcttttc ggagcttgac cgcgcgccgc
     2761 atcatcaaga gccgggaccg acggcatgtg aagatcatag accgctccgc attcgaggac
     2821 cttgtggacg agatggcgca ccttcggcca cgcaatcgac cggccggcga caaatagctt
     2881 ggaccgcgcg ccatcactcg atcatgctgg ttgtggccgc gacgctgtca agccgacatg
     2941 cacgatttgc gtcatggagt agtggttccg cccttgtgct cgatcgtttc cgctcgatcg
     3001 catgtcgcac agtagaaccc gaacagtgcg ggtagccctc cgaggcgcgg aatcgtgcga
     3061 acgagcttca tcggcttttc acactgaggg cagatgggtg gtgtgggtgg atcgcgggac
     3121 atgcagcgct agctaacagc aaagcgcagc tgctaatttg atctagcagt ttacggaaaa
     3181 acctagaaac cgagccattg ttctctcagg tgaggggttg atggttttgg cgatggatcg
     3241 agctttggtc ccttcgattg gccgtggact acgccgcgac cagtttgggg atgcggatca
     3301 gattgtaggc gatcaagttg agcaggaaat cggcagcaac ggcggcgatg ctccagacgg
     3361 tgtcgcggct ccttccggaa ctcgcgctaa ttctcatgac cgctgtcgct acgcacttgg
     3421 tcatgtcatt tgcgcagacg ctgatgcatt acaagctcgg ccacaatcca ataggcggta
     3481 agttcttccg caaccacatc aatttccatc acgcccacta ttcca
//
LOCUS       DATLJT010000996         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3743453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000996 DATLJT010000000
VERSION     DATLJT010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3743453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1029
                     /locus_tag="VM910_22940"
     CDS             292..1029
                     /locus_tag="VM910_22940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI65410.1"
                     /translation="MIDRQVDIETRDGKTTTFITHPERGGPFPVIIFYMDAPAIREEL
                     RDMARRLGTSGYYVMLPNLYYRAGVMELGPLPADPESPERKRMFALMNAINIPLVMED
                     TKALLAYAEADKAANTRIVGALGYCMSGRYAINAATHFPDRVKAAASIYGTHLATEQP
                     DSPHLAVAKTRAELYFACAETDIYAPPEIIEKVRAGMKGSNNEVEIYPGTHHGFAFPK
                     RPVYDRDAAERHWERLVALYRRNLKAA"
     gene            1029..1871
                     /gene="lgt"
                     /locus_tag="VM910_22945"
     CDS             1029..1871
                     /gene="lgt"
                     /locus_tag="VM910_22945"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVI65411.1"
                     /translation="MPFLLIAFPVFDPIAIQIGPIAIRWYALAYIGGIVFGWIYARSL
                     LKNERLWGGPAPISLPQLDDFILWVTIGIILGGRTGYVLFYNLPFFVQHPAEIFELWK
                     GGMSFHGGFLGCVIAVMWFAYKNNISILSLGDITTAVGPIGLFLGRLANFINSELWGR
                     PAEPSLLWAMIFPNGGPLPRHPSQLYEAGLEGILLFTILAVMIRLGALKRPGLILGSF
                     ITLYGLARIVGEHFREPDPQLGFLWGGLTMGMLLSVPMVIAGAILIFLVCRRKSPDKI
                     QAPD"
     gene            1890..>2609
                     /locus_tag="VM910_22950"
     CDS             1890..>2609
                     /locus_tag="VM910_22950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HVI65412.1"
                     /translation="MTEFSPLHSQIKKLIQLSGPMPVWRYMELCLMHPQHGYYVSRDP
                     LGREGDFTTSPEVSQMFGELLGLWAASVWKTIGSPQTLRLIELGPGRGTMMADALRAL
                     RVVPPLHEKLSVHLVEINPVLREKQKSMLSGANSIAWHDSLDEVPEGPSIIFANEYFD
                     VLPVHQALKRENGWHERTVELDATGKLVFGASADPIPRFEVLLPPLVRVAPVGAVFEW
                     RPDSEMMKIATRVRDQDGAALI"
BASE COUNT          471 a          821 c          791 g          526 t
ORIGIN      
        1 ttttcggcct ggttgcgcca gagcgcatcg acctcgcgct tgaccccctg ggctgcgccc
       61 gcggcatcgt tcatcagccg tccgatctcg tcgaaaaacc gattggtcgt ctgcgtcatg
      121 gcgacatctc ctgcgcggga actgaaacaa gaaaatggcg atcgcggcgg cttcatgcaa
      181 ggccctcgca aggccggtac tgccgctcgc cttgtcatgc tcccctttcc ctgcaatcgt
      241 attgaaggtg cacggaatca aaagactcaa agaaaactca acgagacgcc catgatcgat
      301 cggcaagtcg atatcgaaac cagggacggc aagaccacga ccttcatcac ccaccccgag
      361 cgtggcgggc cgtttccggt gatcattttt tacatggacg cgccggcgat ccgcgaggaa
      421 ctgcgcgaca tggcgcgccg gctcggcacg tcgggctatt acgtgatgct gccgaacctc
      481 tattaccgcg cgggcgtgat ggagctcggg cccttgccgg ccgatccgga atctccggaa
      541 cgcaagcgca tgttcgccct gatgaacgcg atcaacattc cgctggtgat ggaagacacc
      601 aaggcgctgc tcgcatatgc cgaagccgac aaggccgcca acacgcgcat cgtcggcgcg
      661 ctcggctatt gcatgagcgg ccgctacgcg atcaacgccg ccacgcattt tcccgatcgc
      721 gtcaaggccg ccgcctcgat ctacggcacg catcttgcga ccgagcagcc cgacagcccg
      781 caccttgcgg tggccaagac cagggcagaa ctctacttcg cctgcgccga gaccgacatt
      841 tacgcgccgc cggagatcat cgagaaggtc cgcgccggga tgaagggatc gaacaacgag
      901 gtcgagatct atcccggcac ccatcacggt tttgccttcc cgaagcgtcc ggtctacgac
      961 cgcgacgcgg cggagcggca ttgggagcgg ctggtcgcgc tctaccgccg caatctcaaa
     1021 gcagcttgat gccctttctc ctgatcgcct ttcccgtgtt cgatccgatc gcgatccaga
     1081 tcggaccgat cgcgatccgc tggtatgcgc tggcctatat cggcggcatc gtgttcggct
     1141 ggatctacgc ccgctcgctg ctcaagaacg aaaggctgtg gggaggaccg gcgccgatct
     1201 cgctgccgca actcgacgac ttcatcctct gggtcacgat cggcatcatt ctgggcggcc
     1261 gcaccggcta cgtgctgttc tacaacctgc ctttcttcgt ccagcatccg gccgagatct
     1321 tcgaattgtg gaaaggcggc atgtcctttc acggcggctt tctcggctgc gtgatcgcgg
     1381 tgatgtggtt tgcgtacaaa aacaacattt cgatcctgtc gctcggcgac atcactaccg
     1441 ccgtcgggcc gatcggcctg ttcctcgggc ggctggccaa tttcatcaac agcgaattgt
     1501 ggggccggcc ggcggagccg agcctgctct gggccatgat ctttccgaac ggcggaccgc
     1561 tgccgagaca tccgagccag ctttatgagg cgggactcga aggcatcctg ctgtttacga
     1621 ttctcgccgt gatgatccgg ctcggcgcgt tgaagcgccc ggggctcatc ctcggcagtt
     1681 tcatcaccct ctacgggctc gcgcggatcg tcggcgagca tttccgcgag cccgaccctc
     1741 agctcggctt cttgtggggc ggcctgacca tgggcatgct gttgtcggtg ccgatggtaa
     1801 ttgccggggc aatccttatt tttttggtat gccggcgcaa atcgccggat aaaattcagg
     1861 ctcccgatta attcgtttca aggcaggttg tgaccgaatt ttcgccgctg cactcacaga
     1921 tcaagaagct gatccaattg tccggcccca tgccggtgtg gcgctatatg gaactgtgcc
     1981 tgatgcatcc gcagcacggc tattacgtct cgcgcgatcc gctcggacgc gagggcgatt
     2041 tcacgacgtc gcctgaagtc agccagatgt tcggggagct tctggggctg tgggccgcct
     2101 cggtgtggaa aacgatcggt tcgccgcaga cgcttcggct gatcgaactc ggcccgggcc
     2161 gcggcaccat gatggcggat gcgctgcgag cgctgcgcgt ggtgccgcca ctccacgaga
     2221 agctgagcgt gcatctggtc gagatcaatc cggtgctgcg cgaaaaacaa aagtcgatgc
     2281 tgtcgggcgc gaacagcatc gcctggcacg attctctcga cgaggtgccg gagggtccgt
     2341 ctatcatctt cgccaacgaa tatttcgacg ttctgccggt tcatcaggca ttgaaacgcg
     2401 agaacggctg gcacgagcgc accgtcgaac tcgacgcgac cggcaagctg gtgttcggcg
     2461 catcggccga tccgattccg agatttgagg ttttgctgcc gccgctcgtg cgtgtcgcgc
     2521 cggtcggcgc ggtgttcgag tggcggccgg attccgagat gatgaaaatc gccacgcgtg
     2581 tgcgcgacca ggatggtgcc gcgctgatc
//
LOCUS       DATLJT010000997         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1445962,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000997 DATLJT010000000
VERSION     DATLJT010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445962"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VM910_22955"
     CDS             <1..334
                     /locus_tag="VM910_22955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65413.1"
                     /translation="IRTGKLRALAVTSAMRSDALPDVPPLGEFLPGYEAVAWYGLCGP
                     RNTPTEVANKLHETTNLALADLKLKARFADLVVEPMPMRPVQLGKFVADETEKWGKVI
                     RAARIKAG"
     gene            complement(552..1676)
                     /locus_tag="VM910_22960"
     CDS             complement(552..1676)
                     /locus_tag="VM910_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HVI65414.1"
                     /translation="MPRPRQFALMLLCGTVTIAGWCAARLDGFEWSAIENTATPIWNR
                     GAARGATISSADAGPVGALLAHAIPSVAAVTTSHRKLPESAVASAAVPAADVVTVDPG
                     TRETAFGLAGVSAAEAPVVEAPLPDSRPTPPPETHGRLVSLFRPDPVEEGPQATARPV
                     ETFDECLVVDICIDEYLWSLYERTPKVDVNKVTERIKTTVKKKGKTRTVTKTITNYVV
                     ADFTWKDPIAAQRGGMSLKDYVIGGMDRGFKLKLFRALRTMDDAGFMPGITSAFRDDY
                     RQSIASGNKAANDSSFHGGSRRGGYGHGLAVDLVSVKGATRLQRFASSEELWKWIDAH
                     ETELGVGRPYRDRDPPHVAAIDGKEYAAKRGRPTVRKAGL"
     gene            complement(2060..>2258)
                     /locus_tag="VM910_22965"
     CDS             complement(2060..>2258)
                     /locus_tag="VM910_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65415.1"
                     /translation="SPFPERAANGEMEMSAQATRLEAAENDLKELMADVTRAIEKAQQ
                     AVSKIAPKTAAAATETQATPR"
BASE COUNT          401 a          717 c          678 g          462 t
ORIGIN      
        1 tatccgcacc ggcaaactgc gcgcgcttgc agtgaccagc gcaatgcgct cggatgcgct
       61 gccggacgtc cccccattgg gagaatttct gccgggctac gaggcggttg cttggtatgg
      121 gctctgcggg ccccggaaca cacccacgga ggtggccaac aagctccacg aaaccaccaa
      181 cctcgctctc gccgatctta agctgaaggc gcgatttgcc gacttagtgg ttgagccgat
      241 gccgatgcga cctgttcaat tgggaaaatt cgtcgccgac gaaaccgaga agtggggtaa
      301 agtaatccga gcagccagga tcaaggcggg gtaaaccttc cgcctccagg ctggaattcc
      361 ataacgtttc gttccgcgaa atccggggcc cagcccgcgc ggcccgtgat gtccaagttg
      421 tcgtaagctg gaaagcacgt cgatgcccgc ggcttcgaga ccatctgaat cttgtgcttg
      481 ctttcttgcg ttcgtgcgca tgcgcctgcg tactaaccct cgcctttacg ccgcttgcgc
      541 gctttcgttg tttacaatcc tgcctttcgc acagtcggcc ggccgcgctt ggcggcatat
      601 tccttgccat cgatcgccgc gacatgcgga gggtctcgat cgcgatatgg gcggccgacc
      661 ccaagctccg tctcgtgcgc atcgatccac ttccacagct cttcgcttga ggcgaatcgc
      721 tgcagacggg tcgccccctt gacgctcacg agatcgacgg caagcccgtg gccatagccg
      781 ccgcggcggc tcccgccgtg aaatgagctg tcgttcgctg ccttattgcc gcttgcgatc
      841 gattgccggt aatcgtcgcg gaatgcgctg gttatgcccg gcatgaaccc ggcatcgtcc
      901 atcgtgcgaa gcgcccgaaa cagcttgagc ttgaagccgc gatccatgcc cccgatcaca
      961 taatccttga gcgacatgcc gcctctttgt gccgcgatcg gatccttcca cgtgaagtct
     1021 gcgacgacat agttcgtgat ggtcttggta acggtccgcg tcttgccctt cttcttgacc
     1081 gtcgtcttga tgcgctcggt caccttgttc acatcgactt tgggtgtccg ttcgtacagt
     1141 gaccagaggt actcgtcgat gcagatgtcg actaccaggc attcgtcgaa tgtttcaacc
     1201 gggcgcgcgg tcgcctgcgg gccctcctcc accggatcgg gcctgaacag ggacacgagc
     1261 cgcccgtgtg tttcgggcgg cggcgttgga cgagagtcgg gcaaaggcgc ctcgacgacg
     1321 ggcgcctcgg cggcggaaac gcctgcaagg ccgaaggcgg tttcgcgcgt gcctggatca
     1381 accgtcacca catcggcggc aggcacggcc gcggatgcga cggcagactc gggcagcttg
     1441 cgatgggacg tcgtcacagc ggccacggag ggtattgcgt gtgccagcag cgcgccgacc
     1501 ggcccggcgt cagcgctcga gattgttgcg ccgcgtgccg ccccgcggtt ccagatcggt
     1561 gttgcggtat tctcgattgc ggaccactcg aagccgtcga gcctcgcagc gcaccatccg
     1621 gcgatagtga ctgttccgca caacagcatc agcgcaaact gccgcggtct cggcatcgct
     1681 cgccccccgt tcggataccg taagacacgc cgcacagcgg gcacacgaca ggatcgattc
     1741 gcaaggcaaa aaagctgcgg gtaattattt tccaaaaagg tgttgtccaa atgcaacgca
     1801 ggtcccgaaa caatgcaaaa cgatgcaagc ttgaaatcgt taccgagata aagcggccgg
     1861 gcggcataaa gattgtaagg attgactgcc gccacacttg gctcgtcgcc tgcgccgtaa
     1921 tcatggccga gcttggccca cccatcccgc tgaggggggt ccctgagcgg cacgccgctg
     1981 gcgagcggac ccgcttggcg accagggagc ttcgatatcg gacttgtcgc gtgttaactt
     2041 caataggcaa cggcagccgt tagcgcgggg tggcttgtgt ctctgttgcg gccgccgcgg
     2101 tcttgggagc tatcttcgac acggcttgct gagccttttc tatggcgcgg gttacgtctg
     2161 ccatcagctc tttcaggtcg ttctcggcgg cctcaagcct cgtcgcctgt gcactcattt
     2221 ccatctcccc attcgccgcc ctctcaggga aagggctc
//
LOCUS       DATLJT010000998         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_67554,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000998 DATLJT010000000
VERSION     DATLJT010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67554"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2234)
                     /locus_tag="VM910_22970"
     CDS             complement(<1..>2234)
                     /locus_tag="VM910_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HVI65416.1"
                     /translation="DFRTEILGLVKAQQVKNAVIVPVGAKGGFVPKRLPSPSNRDAWL
                     AEGTEAYRTLVRSLLELTDNLDGDVVVPPDSTVRHEGDDPYLVVAADKGTATFSDVAN
                     AISAEKNYWLGDAFASGGSQGYDHKKMGITARGAWEAVKRHFRELGTNIQAMPFIAVG
                     VGDMSGDVFGNGMLLSPTTKLVAAFDHRDIFIDPSPDPTISFAERKRLFGLTRSSWQD
                     YDKSLISQGGGVYSRLLKAIPLAPEVRTLLDIDKPQATPFEVMKAILKARADLLWFGG
                     IGTYVRSSGESDDEVGDRANDPIRITGGDVRARVIGEGANLGVTQRGRIEAAQKGVKL
                     NTDAIDNSAGVNTSDVEVNIKIALARPEREGRLSPADRNSLLAAMTDEIAVLVLRNNY
                     LQTLALSLAERKGVSETGFLTRLMHSLEQRGLLNRTVEFLPDDAALSERTRRGQSLTR
                     PELAVLLAYAKLTLYDDLLATTVPDDPYVARELSQYFPREVQDKFPTAVECHRLRREI
                     IATSLVNAVINRGGPACVVRLIDETDTDIPTIVMAYVAVDESYGLKWLNDAIDALDAC
                     IDGQLQLSLYAAVQDLLLSRMVWYVRNVDFKDGLEAVIARFGPAVREIVAGLDTALPP
                     DRQAARSKRRQDLADAGVPIGLAGELADFDALVSAPDIVTVMERASRSIGDAAATFFA
                     AEANFSLDRIIAAARSVPANDYFERLAIDRAIDQIVAAERRLAADMLATGQSGQQAVE
                     TWLT"
BASE COUNT          393 a          670 c          755 g          416 t
ORIGIN      
        1 ccgtgagcca ggtctcgacg gcttgctggc cggactggcc ggttgccagc atatccgcgg
       61 ccagtcttct ttcggctgcc acgatctggt cgatggcgcg gtcgatggcg agacgttcga
      121 aataatcgtt tgccggcacg ctgcgtgcag cagcgatgat gcggtcgaga ctgaaattgg
      181 cttccgcagc gaaaaaggtc gcagccgcat cgccgatgga gcgcgaggcg cgttccataa
      241 ccgtcacgat atccggtgcc gagaccaatg cgtcgaaatc ggcgagttcg ccggcgaggc
      301 cgatcgggac gccggcatcg gcaaggtcct gccgacgctt gctgcgcgcg gcttgccggt
      361 ccggtggcag ggcggtgtca agcccagcga cgatctcgcg gaccgctggg ccgaaacgcg
      421 cgatgaccgc ctcaagaccg tctttgaaat cgacgttgcg cacgtaccag accatgcggg
      481 agagcaagag atcctggact gccgcgtaga gggacaactg caattgcccg tcgatgcagg
      541 cgtcgagtgc gtcgatcgca tcattcagcc acttcagccc gtaggattca tccaccgcca
      601 cgtaggccat gacgatggtt gggatatcgg tatcggtctc gtcgatcagc cgcacgacgc
      661 aggccgggcc accgcggttg atcaccgcat tgacgagact ggtcgcgatg atctcccgtc
      721 gcaggcgatg gcactcgacc gccgtcggga atttgtcctg aacctcgcgc ggaaaatact
      781 gcgatagttc gcgggcaaca tagggatcat caggcacagt ggtggcgagg aggtcatcgt
      841 agagcgtcag cttggcgtag gcgagcaaca cggcaagttc aggccgtgtc agggactggc
      901 cgcgccgtgt acgctcgctg agcgctgcgt cgtcgggcaa gaactccacg gtgcgattga
      961 gcaggccgcg ctgctcgagc gaatgcatca ggcgggtaag gaagccggtc tcggatacgc
     1021 ccttgcgctc ggcgagcgag agcgccagtg tttgcagata gttgttgcgc agcaccagca
     1081 cggcgatttc gtcggtcatt gcggcgagta ggctgttgcg gtcggcgggg ctgaggcgtc
     1141 cctcccgttc ggggcgcgcc agcgcgatct tgatattgac ctcgacgtcg gacgtgttca
     1201 cgccggccga attgtctatc gcgtcggtgt tgagcttgac gcccttctgc gccgcttcaa
     1261 tgcggccgcg ctgggtgacg ccaagattgg ctccttcgcc gatcacccgg gcgcgcacgt
     1321 caccgcccgt gatgcggatc ggatcgttgg cgcggtcgcc gacctcatcg tcgctttccc
     1381 cggaagaacg gacataagtg ccgatgccgc caaaccacag aaggtctgcg cgcgctttca
     1441 gtatcgcttt cattacctcg aacggtgtcg cttgcggctt atcgatatcg agaagggtgc
     1501 gcacctccgg tgcgagcggg atcgccttga gcaggcgcga gtacacgccg ccgccttgcg
     1561 agatcagcga cttgtcgtag tcctgccagc tcgatcgcgt caggccgaac aggcgcttgc
     1621 gttcagcgaa gctgatcgtc ggatcgggtg aggggtcgat gaagatgtcg cggtgatcga
     1681 aagcagccac aagtttcgtg gtcggcgaca gcagcatgcc gttgccgaag acgtcgccgg
     1741 acatgtcgcc cacaccgacc gcgataaacg gcatggcctg tatgttggtg ccgagctcgc
     1801 ggaagtggcg cttgaccgcc tcccaggcgc cgcgcgctgt aatccccatc ttcttgtggt
     1861 catagccctg gctgccgccg gaggcgaagg catcgccgag ccaataattc ttctcggccg
     1921 agatcgcatt ggcgacgtcg gagaaggtcg cggtgccctt gtcggcggct acgaccaggt
     1981 aggggtcgtc gccttcatgg cgcacggtag agtcgggtgg cacaacgacg tcgccgtcga
     2041 gattgtcggt gagctcgagc agcgagcgaa cgagggtgcg ataggcttcg gtgccttcgg
     2101 cgagccaggc gtcgcgattg gaaggcgacg gcaggcgctt ggggacgaag ccgcccttgg
     2161 cgccgaccgg cacgatcacc gcgttcttga cttgctgcgc tttcaccagg ccgaggatct
     2221 cggtgcggaa atcc
//
LOCUS       DATLJT010000999         3944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4202826,
            whole genome shotgun sequence.
ACCESSION   DATLJT010000999 DATLJT010000000
VERSION     DATLJT010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4202826"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1404)
                     /locus_tag="VM910_22975"
     CDS             complement(322..1404)
                     /locus_tag="VM910_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65417.1"
                     /translation="MTAIIAGGADCDLHPAVPHLTSLLPYMNDYWRDQVTTRGMVDLV
                     SQSYPANSPISSRPDWRPAQGKPGSNLDDMRTQALDPFKVGYGILNPLYGVQMVFSED
                     MQDAFCRALNDWLAKEWLEKDKRLRASIVIPTQSVEKSVAEIERCAADHRFVQVLMLV
                     MGDVPLGKRALWPIYATAERLGLPVGIHAGSAYHNPPTSSGWTSYHIEDYVANSQAFQ
                     TQLTSLIVEGVFARYPKLKMVMLESGFTWLAPFLWRLHKFWRGIRMETPWVDRPPMEI
                     VKSNIRFSLQPVDAPPDPAILNRLFDHMQSEELILFSTDYPHWQFEGDDVLPEGLSSD
                     LVRKIMIDNPLATYGRLQQVARETTP"
     gene            1587..2069
                     /locus_tag="VM910_22980"
     CDS             1587..2069
                     /locus_tag="VM910_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVI65418.1"
                     /translation="MQDLVAIATEIAAKLVERKQTIAVAESSTGGLISAALLSVPGAS
                     GYFLGGAVVYTRDARRILMDIPDDAMKGIRSASEPYAKLLANQIRTRFATDWGLSETG
                     ATGPTGNRYGDAAGHSCMAVAGAEITAITLETGRNERLSNMHAFAATALNLLLKELQR
                     "
     gene            complement(2133..3455)
                     /locus_tag="VM910_22985"
     CDS             complement(2133..3455)
                     /locus_tag="VM910_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HVI65419.1"
                     /translation="MARHATPGGLQGARRSIRLHLVAGLAVVAVLAGGLGGWASTQQI
                     SGALIAPGQIVVESNVKKVQHPTGGVVGELRARDGDVVKSGDIVVRLDDTVTKANLAI
                     VTKNLDAALARAARLEAEQRGLDKISFPPQLADRAADPEVAAVIASESKLFDVRVTGR
                     AGQKAQLSERIRQLKEEIEGLSAQERAKEQEIALVQQELSGVRDLYEKRLVQISRLTT
                     LERDAARLNGERAQYIAARAQAKGKITETELQIIQVDRDVVSEVSKDLRETNDKIGEL
                     IERKVTAEDQLRRIDIRAPQDGIVEQSTVHTVGGVITAGDAIMLIVPQTDDLQVEAKV
                     NPQDIDKLQVGQKTLLRLSAFNQRTTPELNGFVSRVSPDVTTEQRTGQSYYTIRVSMP
                     PQEIARLGDVKLIPGMPVEAFVQTGERTLISYLMKPLSDQLMRTFREK"
     gene            complement(3473..>3944)
                     /locus_tag="VM910_22990"
     CDS             complement(3473..>3944)
                     /locus_tag="VM910_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI65420.1"
                     /translation="NICRFDPDAKSETIIKAAREAGVHDMIIKMRDGYNTQVGEQGAA
                     LSAGQAQRVALARALYGDPFLIVLDEPNSNLDTEGDEALTRAIRSARERGAIVVVVAH
                     RPVGIEAVDQILVLRDGHVQAFGPKEQVLAQVLQPRIATPTPIKIVPDAGVAKS"
BASE COUNT          682 a         1289 c         1223 g          750 t
ORIGIN      
        1 gggtggccga aattcccaat gcgtgatcaa ccatgggatg acgcccgcca acatcgccgg
       61 tgcaacgacg aaaaacagcg cggaacccag gattgctgtg atcttcgaca tgcgtccccc
      121 taatgttaag caagcgtagg agggcaaggc ggccccggag tttcgattag tgcacctact
      181 tggggtccgc cttgcccacc ctacgccaac cgtaatcgcc cgcaatgtct cttgagggtc
      241 aaagggcgac attcccgtgc cgttggcgca tttccgcttt accccacaaa gcagacatcg
      301 actgtttgag agtacaagag ttcatggcgt ggtttccctg gcgacctgtt ggagccggcc
      361 gtaggtcgca agcggattat cgatcatgat cttgcgaacg agatcggacg acagtccctc
      421 cggcagaacg tcatcgccct cgaattgcca atgcggatag tcggtcgaga ataggatcaa
      481 ttcctccgac tgcatatggt cgaacagccg gttgagaatc gccggatccg gcggggcatc
      541 caccggttgc agcgaaaagc ggatgttgct cttcacaatc tccatcggcg ggcgatcgac
      601 ccagggcgtc tccatgcgga tgccacgcca gaacttgtgc agccgccaga ggaacggcgc
      661 cagccacgta aaaccgctct ccagcatcac cattttcagc ttcggatacc gcgcgaacac
      721 gccctcgaca atcaggctgg tgagctgggt ttggaacgcc tgcgaattgg cgacatagtc
      781 ctcgatgtga taggaggtcc agccgctcga cgtcggcggg ttgtgatagg ccgagccggc
      841 atggatgccg accggcagcc ccaaccgctc ggcggtcgca tagatcggcc acaacgcgcg
      901 cttgcccaac ggcacgtcgc ccatgaccag catcagcacc tggacgaagc ggtgatcggc
      961 cgcgcaacgc tcgatctcgg cgacggattt ttccacgctc tgtgtcggga tcacgatcga
     1021 agcgcgcagc cgcttgtcct tttcgagcca ctccttcgca agccagtcgt tcagcgcccg
     1081 gcagaaggcg tcctgcatgt cctcggaaaa caccatctgc acgccgtaga gcggattcag
     1141 gatgccgtag ccgaccttga acggatcgag cgcctgcgtc ctcatgtcgt ccaggttcga
     1201 gccgggcttt ccttgcgcgg ggcgccagtc gggtcgcgac gagatcggcg aattggccgg
     1261 ataggattgc gacaccagat cgaccatgcc gcgcgttgtc acctggtcgc gccagtagtc
     1321 gttcatgtaa ggcaagaggc tggtcaagtg cggaacggct gggtgcaggt cgcaatccgc
     1381 gccgccggcg atgatagccg tcatcgggct cttttcctct gctcctgagc gctttgcctc
     1441 ggtgatgccg acggccattg aagcaggccg gcttccgccc cgcaactcgg caagccatgc
     1501 aatcctgtgc tagtggtccg attccaacat cgctcccgat ccggggagcc ggtttttcga
     1561 atgtcactcg caccgggagg accgacatgc aggacctcgt tgccatcgcc acagagatcg
     1621 ccgcaaagct ggtcgagcgc aagcagacca tcgcggtcgc ggagtcctcg accggcggcc
     1681 tgatctccgc ggccctgctc tcggtgcccg gcgcctccgg ctatttcctc ggcggcgcgg
     1741 tggtctatac gcgcgacgcg cggcgtattc tgatggatat ccctgatgat gcgatgaaag
     1801 gcatccgctc ggcgtcggag ccttacgcaa agttgctggc gaaccagatc cgcacgcgtt
     1861 ttgcgacgga ttggggcctc tcggagaccg gcgcgacggg acccaccggc aaccgctacg
     1921 gcgatgccgc cggccatagc tgcatggcgg ttgcgggcgc cgagatcaca gccatcacgc
     1981 tcgagaccgg ccgcaatgag cggctttcca acatgcatgc gttcgcggcg acggcgctca
     2041 atctgctgct gaaggagttg cagcggtagc aattttacac ggctgtcgtc caggccggga
     2101 cgacggcgga aatttgccgg gaaacgagcg actcacttct cccggaaggt acgcattagc
     2161 tggtcgctga gcggcttcat cagataggag atcagggtgc gctcgccggt ctgcacgaac
     2221 gcctccaccg gcatgcccgg gatcagcttg acgtctccga gccgcgcgat ctcctggggc
     2281 ggcatcgaga cacgaatggt gtaatagctc tggccggtcc gctgctcggt ggtgacgtcg
     2341 ggcgagacgc ggctcacgaa cccgttcagc tccggcgtgg tgcgctggtt gaaggccgac
     2401 agacgcaaca atgttttctg cccgacctgc agcttgtcga tatcctgcgg attgaccttg
     2461 gcctcgacct gcagatcgtc agtctgcggc acgatcagca tgatggcgtc gccggccgtg
     2521 atcaccccgc cgacggtatg cacggtcgac tgttcgacga tgccgtcctg cggggcgcgg
     2581 atgtcgatcc gacgcaactg atcttccgcg gtgaccttgc gctcgatcaa ttcgccgatc
     2641 ttgtcgttgg tctcgcgcag gtcctttgag acctcgctga ccacgtccct gtcgacctga
     2701 atgatctgca gctcggtctc ggtgatcttg cccttggcct gggcgcgcgc ggcgatatat
     2761 tgcgcgcgct cgccgttcag ccgtgccgcg tcgcgctcga gcgtggtgag tcgcgaaatc
     2821 tgcaccagcc gcttctcgta aagatcgcga acgccgctca gttcctgctg gaccagcgca
     2881 atctcctgct ccttcgcccg ttcctgggcg gaaagcccct cgatctcttc cttgagttgc
     2941 ctgatgcgct cgctcaattg cgccttttgc ccggcgcggc cggtaacgcg cacgtcgaac
     3001 aatttggact cgctcgcgat cacggcggcg acctcggggt cggctgcacg atcggcgagc
     3061 tgcggcggga aactgatctt gtcgaggccg cgctgctcgg cttccagccg cgccgcgcgc
     3121 gccagcgccg catcgagatt tttggtgacg attgcgagat tggccttggt gacggtatcg
     3181 tcgagccgca ccacgatatc gccgctcttc accacgtcgc catcgcgggc gcgcaactcg
     3241 ccgaccacgc cgccggttgg atgctgcacc ttcttgacgt tggactcgac cacgatctga
     3301 cctggcgcga tcagcgcgcc ggaaatctgc tgcgtcgagg cccagccgcc aagaccgccg
     3361 gcaagtacgg cgacgaccgc gagtcccgca accaggtgca gccggatcga gcggcgcgcg
     3421 ccctgcagac cacccggcgt cgcgtgacgc gcaatttgcc tgccggcttc gctcacgact
     3481 tggccactcc cgcatccggt acgatcttga tcggagtcgg cgtagcgatc cgcggctgca
     3541 gcacctgcgc cagcacctgc tccttgggac cgaaggcctg gacgtggccg tcgcgcagga
     3601 ccaggatctg gtcgaccgcc tcgatgccca ccggcctgtg cgcgaccacc acgacgatgg
     3661 cgccgcgctc gcgcgcgcta cggatcgcgc gcgtcagcgc ctcatcgccc tcggtgtcga
     3721 gattggaatt gggttcgtcc agcacgatca ggaacgggtc gccgtaaagc gcgcgcgcca
     3781 aggccacgcg ctgcgcctgc cctgccgaca atgccgcgcc ctgctcgccg acctgggtgt
     3841 tgtagccgtc gcgcatcttg atgatcatat cgtgcacgcc ggcttccctg gcggccttga
     3901 tgatggtttc ggatttcgcg tcgggatcga accggcagat gttc
//
LOCUS       DATLJT010001000         5401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3284530,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001000 DATLJT010000000
VERSION     DATLJT010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284530"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VM910_22995"
     CDS             <1..317
                     /locus_tag="VM910_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding molybdopterin dehydrogenase"
                     /protein_id="HVI65421.1"
                     /translation="AALKRRSAFRQISLAPAGRSAALLIGSLTSDDTLLLTVTASTKR
                     PVRLSFDRIVDPGAMRQAILQAIPDGIYHDDVHGKPLWRKHMTLRLAEKIRSELLGAG
                     RS"
     gene            314..3037
                     /locus_tag="VM910_23000"
     CDS             314..3037
                     /locus_tag="VM910_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI65422.1"
                     /translation="MTFEINGKAFSQTPRPGQCLRTFLRELGHFGVKKGCDAGDCGAC
                     TVLLDGEPVHSCLIPAYRAENHAVTTIEGFAGDGGTHPMQQAFLDAQAFQCGFCTGGM
                     ILTCASLNQAQRQDLGASLKGNICRCTGYRAIEDALDGRHNIEEVGAGEAFGRSLPAP
                     AGPLVVKGAARYTFDTEIDGLLHIKLARSPHPHARIRSIDKSAALALSGVHAVLTFED
                     APHALFSTARHEKAWMDPNDTRVLDNVVRFVGQRVAAVVADTEAAAEEGCRRLRVDYE
                     LLPAVVDADQAIAPGAPVIHGDKTSEHRVKNAARNIVAEAHGEFGNVADALAASAATY
                     EGTFTTQRVQHAALETHGGLAWIDAQGMLNVRSSTQVPFLTRRALSEIFDLAPEKVRV
                     FCERVGGGFGGKQEMFVEDILALAALKTGRPVKLELTREEQFIATSTRHPMRVTVKAG
                     ADKDGKLTALQLDVLSNTGAYGNHAGPVLFHGCGESISVYNCPNKKVDAVAAYTNTVP
                     AGAFRGYGLPQTVFAIEAAIDELATQLGIDPFEMRRRNVVKPGDPMLSPQGYGASDVL
                     YGSYGLDQCLDLVERAMRAEAPNPGLSPEWLIGDGIALTMIDTVPPDGHIADSVIALC
                     DDGSFELIVGTAEFGNGTSTVHRQIAATALATTVDRIRLKQSDTANGGHDTGAYGSTG
                     TFVAGRATQAAAENLAAELEAFAALALGADAGACALEDNSAVCGNKRLSFAQLANAAR
                     EQGRTLLANGTSAGTPRSVAFNVQGFRVAVNKGTGEIKILKSVQAADAGRVANPMQCR
                     GQVEGGVAQSLGATLYEEMVIDEGGRVINPRFRDYHLPQFADVPRTEVYFADTSDTLG
                     PMGAKSMSESPYNPVAAALGNALRDATGIRFTSVPFKPDRLFPLLHEKFGP"
     gene            3127..4524
                     /locus_tag="VM910_23005"
     CDS             3127..4524
                     /locus_tag="VM910_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HVI65423.1"
                     /translation="MSSEIHPVDEVLPVPRLLALGLQHVLVMYAGAVAVPLIVGRALK
                     LPPEDVAFLISADLFACGLATLVQCVGFPGVGIRLPVMMGVTFASVSPMLSMAASPEI
                     GLLGIYGSVIAAGLFGILVAPFVSRLLPLFPPVVTGTIILVIGISLMRVGINWAGGGL
                     PTLTRVVDGVPGAFPNPAYGQLQGLGISAFVLLFVLGLIKWGSGFVENVAVLLGIVAG
                     ALLASILGVMHFDKVIAAPWADIVIPLRFGVPQFHLVPIITMCIVMIVVMIESLGMFL
                     ALSEITGKAVDRDALSRGLRADGVGTLLGGIFNTFPYTSFSQNVGLVGVTGVRSRWVT
                     VTGGCIMLLLGLLPKLAALVEAVPLVVLGGAGLVMFGMVAATGARILTAVDFKTNRHN
                     LFVVAISIGFGMIPLAAPGFFKNLPHDLQPLLESGILLCAVVAVLLNAFFNGIGGGKA
                     AEVDAAAAASSAQHV"
     gene            complement(4531..>5401)
                     /gene="uraD"
                     /locus_tag="VM910_23010"
     CDS             complement(4531..>5401)
                     /gene="uraD"
                     /locus_tag="VM910_23010"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HVI65424.1"
                     /translation="SDLNACSKADFVAALANIFEYSPWIAEQAADARPLAGVKALFEA
                     MKAAVARAPAELQLALIKAHPDLANKTQRATGLTADSTAEQNSVGLDRLSDAEYEAFE
                     RVNNAYRAKFGFPYIVCVRRQTKDSILRDFERRLPNDANKEIRTAIEEICRIAALRLD
                     QQVVSDDRLQVHGRLSTHVLDTHSGKPAAGIALELVELSELGSSRPITRAITNSDGRT
                     DQPLIGGRPVPIGRYELSFSVGAYFRERNVPMSDPPFLDRIPLRFAVSEPEGHLHVPL
                     LVTPWSYATYRGS"
BASE COUNT          915 a         1754 c         1733 g          999 t
ORIGIN      
        1 tcgccgcatt gaaacggcgc tcggcgttcc gccagatctc gctggcgccg gccggccgtt
       61 cggcggcgct cctgatcggg agcctcacca gcgacgacac cttgctgttg accgtgacgg
      121 cctcgaccaa gcggccggtc cgcctgtcct tcgaccgaat tgtcgacccg ggcgcgatgc
      181 gccaggccat cctgcaagcc attccggacg gtatttatca cgatgacgtc cacggcaagc
      241 cgctgtggcg caagcacatg accttgcggc ttgcggaaaa gatacgctcc gaactcctgg
      301 gcgcggggcg gtcatgacat tcgagataaa cggcaaggcc ttttcacaga cgccgcggcc
      361 cggacaatgc ctgcgaactt ttttgcgcga gctcggtcat ttcggcgtca agaaaggctg
      421 cgacgccggc gactgcggcg cctgcaccgt gctgctcgac ggcgagcccg tccatagctg
      481 cctgatcccg gcgtaccgcg cagagaacca cgcggtcacg accatcgagg ggtttgcggg
      541 cgatggcggc acgcatccga tgcagcaagc cttcctcgat gcgcaagcct tccagtgcgg
      601 cttctgcacc ggcggcatga tcctgacctg cgcttcgctg aatcaggcgc agcggcagga
      661 cctcggcgcc tcgctgaagg gcaacatctg tcgctgcacc ggctatcgcg cgatcgagga
      721 tgcgctcgac ggcaggcaca atattgaaga ggtcggagcc ggcgaagcct tcggccgcag
      781 cctgcccgcg ccggccggac cgctcgtggt caaaggcgcg gcgcgttaca cctttgacac
      841 cgagatcgat ggccttttgc acatcaagct cgcgcgttcg ccgcacccgc acgcgaggat
      901 cagatcgatc gacaagagcg cggcgcttgc gctgtccggt gtgcatgcgg tcctgacctt
      961 cgaggatgcg ccccacgcac tgttctccac ggcacgtcat gagaaggcct ggatggatcc
     1021 gaacgacacc agggtgctcg acaatgtcgt gcgctttgtc ggccagcgag ttgcagccgt
     1081 cgtcgccgac accgaagccg cggcagaaga gggctgccga cgattgcggg tcgactacga
     1141 gctattgccg gccgtcgtcg atgcggacca ggcgatcgcg ccgggcgcgc cggtgattca
     1201 cggcgacaag acgagcgaac atcgcgtcaa gaatgccgct cgcaacatcg tcgccgaggc
     1261 gcatggcgag ttcggcaatg tcgccgacgc gctggccgcc tccgccgcca cctatgaggg
     1321 caccttcacc acccagcggg tccagcacgc agcactggag acgcatggcg ggctcgcctg
     1381 gatcgacgcg cagggcatgt tgaacgtccg ctcctcgacg caggtgccgt tcctgactcg
     1441 ccgcgcactg agcgagatct tcgatcttgc accggagaag gtgcgcgtgt tctgcgaacg
     1501 cgtcggcggc ggtttcggcg gaaagcagga gatgttcgtg gaggacattc tggcactggc
     1561 ggcgctgaaa accggacgcc cggtcaagct cgaactgacc cgcgaagagc agttcatcgc
     1621 gacctcgacg cggcatccga tgcgggtcac ggtgaaagcc ggcgccgaca aagacggcaa
     1681 gctgactgcg ctacagctcg acgtgctctc caacaccggc gcctacggca atcacgccgg
     1741 ccccgtcctg tttcacggct gcggcgagag catcagcgtc tataactgtc cgaacaagaa
     1801 ggtcgacgcc gtcgccgcct ataccaacac cgtgcccgcg ggcgcgttcc gcggctatgg
     1861 tctgccgcaa acggtgtttg cgatcgaggc cgcaatcgac gaactcgcga cacagctcgg
     1921 catcgaccct ttcgagatgc gccgccgcaa cgtcgtcaag ccgggcgatc cgatgttgtc
     1981 gccacagggc tacggcgcaa gcgacgtgct ctatggctcc tacggtctcg atcagtgcct
     2041 cgatctggtc gagcgcgcga tgcgggccga agcgccgaac ccgggccttt ctccggagtg
     2101 gctgatcggc gacggcatcg cgctgaccat gatcgatacg gtcccgccgg acggtcatat
     2161 tgcagacagc gtcatcgcgc tctgcgacga cggcagcttc gagctcattg tcggcacggc
     2221 ggagttcggc aacggcacca gcacggtgca tcgccagatc gccgcgacgg cacttgcgac
     2281 caccgtcgac cggatccgac tgaaacaatc cgacaccgcc aatggcgggc acgacaccgg
     2341 cgcttacggc tcgaccggca cattcgtcgc cggccgcgcg acgcaggcgg cagcggaaaa
     2401 tctcgccgcg gagctcgagg cttttgcggc gctggcgctc ggtgctgacg caggtgcctg
     2461 cgcgctcgag gacaattccg ccgtctgcgg caacaagcgg ctatccttcg cacagcttgc
     2521 caacgctgcg cgcgagcaag gtcgcacgct cctggcgaac ggcacctcgg ccggcactcc
     2581 gcgctcggtc gcgttcaacg tgcagggttt tcgggttgcc gtgaacaagg gcacaggcga
     2641 gatcaaaatc ctcaaaagcg tgcaggcggc ggatgccggg cgggtcgcca atcccatgca
     2701 atgccgcgga caggtcgaag gcggagtcgc gcagtcgctg ggggcgacac tgtacgaaga
     2761 gatggtgatc gatgaaggcg gccgcgtgat caaccccaga ttccgcgatt atcatctgcc
     2821 gcaatttgcc gatgtgccgc gtaccgaggt ctatttcgcc gacacttccg acacgttggg
     2881 accgatgggc gccaaatcga tgagcgaaag cccgtataat ccggtggcgg cggcgctcgg
     2941 aaatgctttg cgcgacgcca cgggtatccg cttcacctcg gtaccgttca agccggaccg
     3001 gctttttccg ctcctgcatg agaaattcgg accgtaatag accggggcct gcgctgcttg
     3061 gcatgggcct tgaatgaccg ataatagcgg gcgcgagtcc aacgatttgc ggacaggggc
     3121 aaatccatga gcagtgaaat tcatcccgtc gacgaagtct tgccggtccc gcggctgttg
     3181 gcgctcggcc tgcagcacgt gctcgtgatg tatgccggcg cggtggcggt tccgctgatc
     3241 gtcggccgcg cgctcaagct gccgcccgag gacgtcgcct ttttgatcag cgcggacctg
     3301 tttgcctgcg ggctggccac gctggtgcag tgcgtcggct ttccgggcgt cggcatcagg
     3361 ttgcccgtca tgatgggcgt tacctttgcc tccgtcagcc cgatgctgtc gatggctgcc
     3421 agccccgaga tcggactcct cggcatttat ggctcggtca tcgctgccgg actcttcggt
     3481 atccttgtgg cgcccttcgt cagccgcctg ctgccactgt tcccgcctgt cgtgaccggc
     3541 accatcatcc tggtgatcgg aatttctctg atgcgggtcg gcatcaactg ggcgggcggc
     3601 ggcttgccca ccctgaccag ggtggtcgac ggcgtccccg gcgccttccc caatcccgct
     3661 tatgggcaac tgcagggcct tggcatttcc gccttcgtgc tgctgttcgt ccttggcctg
     3721 atcaagtggg gatccggttt cgtcgagaat gtcgcggtgc tgctcggtat cgttgccggc
     3781 gcccttctcg cgagcatcct cggtgtcatg catttcgaca aagtcatcgc cgctccgtgg
     3841 gctgacatcg tgatcccgct ccgcttcggc gtgccgcaat tccatctcgt gccgatcatc
     3901 accatgtgca tcgtgatgat cgttgtcatg attgaatcgc tcggcatgtt cctggccctc
     3961 agcgagatca cgggcaaggc cgtcgaccgc gatgcgctgt cccgcggttt gcgcgccgac
     4021 ggcgtcggga cgctgctcgg tggcatcttc aacacgttcc cctatacgtc gttctcgcag
     4081 aatgtcgggc tcgtcggcgt gaccggcgtg cgctcgcgct gggtcactgt gaccggcggc
     4141 tgcatcatgc tgctgctcgg ccttttgccg aagctcgcgg cgctggtcga agcggtgccg
     4201 cttgtcgtgc tcggcggggc aggccttgtg atgttcggca tggtggctgc gaccggcgca
     4261 cggatactca ccgcagtcga tttcaagacc aaccgccaca acctgtttgt ggtcgcgatt
     4321 tccatcggct tcggcatgat cccgctggca gcgcctggct tcttcaagaa cctgccgcac
     4381 gacctgcagc cgttactcga atccggcatc ctgctctgcg ccgtcgtcgc ggtgctcctc
     4441 aatgcattct tcaacggtat cggcggcggc aaggctgccg aagtcgacgc cgcagccgcg
     4501 gcatcgtcgg ctcagcatgt atgaacgcga tcaactgccg cgatacgtcg cgtagctcca
     4561 gggcgtgacc aggagcggca cgtggagatg gccttcgggc tcgctcacgg caaagcgcaa
     4621 ggggatgcgg tcgaggaacg gaggatcgga catcggcaca ttgcgttccc tgaaatacgc
     4681 accgacgctg aagctcagct cgtagcggcc gatgggcacc ggacggccgc cgatcaaggg
     4741 ctgatcggtg cggccgtcgg agttggtgat cgcgcgcgta atcgggcggc ttgaaccgag
     4801 ttcggacagt tcgaccagtt ccagcgcaat tcctgccgcg ggtttgcccg aatgcgtatc
     4861 cagcacatgg gtcgacagcc gcccatgcac ctgcagccgg tcatccgaga cgacctgctg
     4921 atcgagccgc aacgcggcga tccggcagat ctcctcgatc gcggtccgga tttccttatt
     4981 ggcatcgttc ggcagccggc gttcgaaatc gcgcaggatg gaatccttgg tctggcggcg
     5041 gacgcagacg atgtagggaa agccgaattt cgcgcgatag gcattgttga cgcgctcgaa
     5101 ggcctcatat tccgcgtccg acaggcgatc gagaccgacg ctgttctgct cggcggtcga
     5161 gtccgccgtg agcccggtgg cgcgttgcgt cttgttggcg agatcgggat gcgccttgat
     5221 cagcgccaac tgaagctccg ccggcgcacg cgcaacggcc gccttcatcg cctcgaataa
     5281 tgccttgacg ccggcaagcg gacgcgcgtc ggccgcctgc tcggcaatcc atggcgaata
     5341 ctcaaagata ttggcgagcg ccgcgacgaa atcggctttg ctgcatgcgt tgagatcgga
     5401 a
//
LOCUS       DATLJT010001001         3696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1446280,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001001 DATLJT010000000
VERSION     DATLJT010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446280"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VM910_23015"
     CDS             <1..264
                     /locus_tag="VM910_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65425.1"
                     /translation="GQRVALGGGRKFERLERTNALTQFVTDMQGRVESLSRVGLGGKG
                     PVLNPELVVAQYLAGSQRRFDFGPNPPISIRFAARSDPVDFTS"
     gene            357..842
                     /locus_tag="VM910_23020"
     CDS             357..842
                     /locus_tag="VM910_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65426.1"
                     /translation="MADAERRKCKCCHKLFRPDPRNRHHQRYCAAPSCRAASKAASQA
                     RWLAAPENRDYFRGPVNVARVRAWRSRNPGYWRKRRRARVALQELSLVQDVDFTNKKV
                     IFAGLPLQEVLSAQPAVLIGLIAHITGSPLQEEILRTTDRLLQLGQDILATSAGSGRP
                     P"
     gene            870..2345
                     /gene="istA"
                     /locus_tag="VM910_23025"
     CDS             870..2345
                     /gene="istA"
                     /locus_tag="VM910_23025"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI65427.1"
                     /translation="MIDYETFCKIHDCHHRQGLTIAQTARALGLHRSTVAIWLVRARF
                     TRRRSQPRRSKLDPFKPSITRLLDTHPYSAQQIFQRLCEEGYRGGVSILRDYVRRIRP
                     PQRPVYLKLHFAPGECAQVDWGAFGSIAVGNTRRRLSFFVMVLAFSRQLYVEFTVSQT
                     MEHFLACHEHAFTAFGGVPAKVMVDNLKSAVLQRLAGVAPVFNPRYLDFARHHGFAIE
                     PCNVARGNEKGRVESGVGYVKKNLLNGLELTEFAAIQAAAQLWLDTIANVRIHGETQQ
                     RPIDLLMQERPRLSPLNPHPYDIARTFTSVASSQFRITLDPNHYSVPVSYAHRKLTVK
                     AYPDRICIYFDNQLIARHTRHYGRHQDIEDPDHAKALIVQRRRAGEQRLLMRFLALSP
                     DAAAYYAGLEERRFNARHHLRKILALAEIYPVDLVARAISDGLAFEAFSAEYITNILE
                     TRTRALPEPGPLQLTRRHDLLDIDIDPPDLNDYKVNDHDTE"
     gene            2332..3081
                     /gene="istB"
                     /locus_tag="VM910_23030"
     CDS             2332..3081
                     /gene="istB"
                     /locus_tag="VM910_23030"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI65428.1"
                     /translation="MTPSDQTQLESQLQRLHLGYVQSNYKTLARKAAEQQRSHLDYLA
                     QLVEGEFTMREMRSIERRIKNARFPVRKTLDDFQWSWPKKINRLQIQNLFRLDFIATC
                     TNLVMIGNVGLGKTHLSIALGHAACTGGHSVLFTTAVDVINTLAAAQSAGQLKREIRR
                     YLKPAVLIVDELGYLPIDKHGADLLFQIISERYERAPIVITTNRAYKHWAQIFNNDST
                     LTSAILDRVMHHVDTVVIEGKSFRMKDEVEE"
     gene            3090..3302
                     /locus_tag="VM910_23035"
     CDS             3090..3302
                     /locus_tag="VM910_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65429.1"
                     /translation="MTRTRSFELPDYRVSDEAAAYIADTLMQIALQFEATHFAQIRRH
                     YQSLGPQPPDDNPGQQLDLFDKHPLF"
BASE COUNT          751 a         1228 c          979 g          738 t
ORIGIN      
        1 ggtcagcggg tcgcgctggg cggcggtcgc aagttcgagc gcctcgaacg gaccaacgca
       61 ctgacccaat tcgtcacaga tatgcagggt cgggttgagt ccctgagccg tgtgggcctc
      121 ggcggaaagg gcccggtgct caatcccgag ctcgtcgtag cgcagtatct tgccggatct
      181 cagcgccgat ttgattttgg accaaaccca ccgatttcaa ttcgcttcgc ggcgcgttcc
      241 gatcctgtgg attttacgtc gtgagattgc gccagagttg acgactgcaa tctcatctgg
      301 agagcagtat ccgtctctgc gttggctgat gatgtgtctc ggcaggaggg gacgcgatgg
      361 ccgatgcgga acgtcgtaaa tgcaagtgct gtcacaagtt atttcgcccc gatccacgta
      421 accggcatca ccagagatac tgcgctgcgc ccagctgtcg cgcggccagc aaagccgcca
      481 gccaggcccg ctggctcgcc gctcccgaga atcgggacta tttccgcgga ccagtgaatg
      541 tcgcccgggt cagggcatgg cgatcacgca atcccggcta ctggcgcaag cggcggcgcg
      601 cccgcgttgc gttacaagag ctctcattgg tgcaagacgt tgattttacg aacaaaaaag
      661 tcattttcgc gggcctgccg ttacaagagg tcttatcggc gcaacccgct gttttgattg
      721 gtttaatcgc ccatattacg gggtcaccgc tgcaagaaga gatcctccgc accaccgacc
      781 gtttgctaca gctgggtcaa gacattctcg caacttccgc gggctcggga cggcccccat
      841 gagcgaggct aacagcagcg ggcacggtcg tgatcgatta cgagacattc tgtaagatcc
      901 acgattgtca ccatcgccaa ggcttgacga tcgcgcagac cgctcgcgcc ctcgggcttc
      961 atcggagtac ggtcgcgatc tggttggtgc gagcgcgctt tacacggcga cgcagccagc
     1021 cgcgtcgcag caaacttgat ccgttcaagc cgagcatcac gcgcttgctc gacacccatc
     1081 cctacagcgc tcagcagatt tttcagcggc tatgcgagga aggatatcgc ggcggcgtca
     1141 gcatcctgcg cgactacgtc cgccgcattc gcccgcctca gcgcccagtc tatctgaagc
     1201 tgcattttgc tcccggcgag tgcgcacagg tcgactgggg cgccttcgga agcatcgccg
     1261 tcggcaatac ccgacgtcgg ctttcgttct ttgtcatggt gctggccttc agccgacaac
     1321 tgtatgtcga gttcaccgtc tcgcagacga tggagcattt tctcgcctgc cacgaacacg
     1381 cgttcacggc gtttggcggt gtgccggcca aagtcatggt cgacaatctt aaatcggcag
     1441 tcctgcagcg cctggctggc gtcgcaccgg tgttcaatcc gcgttacctc gatttcgctc
     1501 gccaccatgg ctttgccatt gagccctgca acgttgctcg gggcaatgag aaggggcgcg
     1561 tcgagtccgg ggtaggctat gtcaaaaaga atctcctcaa cggcctcgag ctgaccgaat
     1621 ttgccgccat ccaagccgcc gcacagctct ggctcgacac catcgccaac gtgcgcatcc
     1681 atggcgagac gcagcagcga cccatcgatc tgctgatgca ggagcggccg cgtctttcgc
     1741 ctctcaaccc acacccctat gacatcgcac gcaccttcac cagtgtggct tcgagccagt
     1801 tccgcatcac cttggacccc aaccattatt ctgttcccgt cagctatgcg catcgcaaat
     1861 tgacggtcaa agcctatccg gatcgcattt gcatctactt cgataaccag ctcatcgccc
     1921 gccacacacg ccactatggt cggcatcagg acatcgaaga ccccgaccat gccaaagctc
     1981 tgatcgttca gcgccggcgc gccggcgaac agcgcttgct gatgcgcttt ctcgccctct
     2041 cgcccgacgc agcagcctat tacgccggct tggaagagcg caggttcaac gcccgccatc
     2101 acctccgcaa aatcctggcg ctggcggaaa tctatcccgt cgacctcgtg gcgcgcgcca
     2161 tctccgacgg gctcgccttc gaggccttca gcgccgaata catcaccaac atcctggaga
     2221 cccggacccg ggccttgccc gaaccagggc cgctgcaact cacccgccgc cacgatctgc
     2281 tcgacatcga tatcgatccc cccgatctca acgactacaa ggtgaacgat catgacaccg
     2341 agtgatcaga cgcaattgga atcccagctg cagcggctcc acttgggcta tgtccaaagc
     2401 aactacaaaa ccttggcccg caaagccgcc gaacagcagc gctcgcatct cgattatctc
     2461 gcgcagctcg tcgagggcga gttcacgatg cgcgagatgc gcagcatcga acggcgaatc
     2521 aagaacgccc gctttcctgt ccgcaaaacc ctcgatgact tccaatggag ctggcccaaa
     2581 aagatcaatc gcctgcagat ccagaacctg tttcgtctcg atttcatcgc aacatgcacc
     2641 aacctcgtga tgatcggtaa cgtcggtttg ggcaaaaccc acttatcgat cgccttgggc
     2701 catgccgcct gcaccggcgg gcactccgtg ctcttcacca ccgccgttga tgttatcaac
     2761 accctcgccg ccgcgcagtc cgccggtcaa ctcaagcgcg agatccgccg ttatctgaag
     2821 cccgcagtcc tcatcgtcga cgaactcggc tatctcccca tcgacaagca cggcgcagac
     2881 ttgctgttcc agatcatcag cgagcgctac gagcgcgccc cgattgtgat tacgaccaac
     2941 cgcgcctaca agcactgggc acagatcttc aataatgaca gcacgctgac ctccgccatt
     3001 ctcgatcgcg tcatgcacca cgtggacacc gtcgtcatcg aaggcaaaag cttccgcatg
     3061 aaggacgagg tcgaagagta gaggatgcaa tgacgcgaac gcgctcgttc gaattgcccg
     3121 actaccgcgt ctcagatgag gccgccgctt atatcgccga cacactcatg cagatcgccc
     3181 tgcaattcga ggcaacccac ttcgcccaaa tccgccgtca ttaccagtcc ctcgggccac
     3241 aacctcctga tgacaatccc ggtcagcagc ttgatctatt cgacaaacac ccgctgtttt
     3301 gagcgcccac cgcgcgcacc gacgggcacg ccttctcttg cttcagtaac tttcttttgt
     3361 tcgaccgcgc cgcaggcttt cttccgcccc gaccgtagat ttcgcacgcc gtcgcgcgca
     3421 ggggctgtca aggtcggccg gcactccgcc ggtaaaggca atctccggtg tttccaggcc
     3481 gcaccttgac agccccgagc acggcggcag catcggtcag tcggggcaag gagccacacc
     3541 ccggcaacag cagcgcctag aatttcaact cgccgcttcg ataatttcaa accggtggtt
     3601 tttgatcaat ttctcgccgg tgatgacaga aggaaatgct tccgtgatct gacgggcaac
     3661 ccattccgcc gtcggattct ttgtgacgtt gatcca
//
LOCUS       DATLJT010001002         1544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3744055,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001002 DATLJT010000000
VERSION     DATLJT010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744055"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VM910_23040"
     CDS             <1..1469
                     /locus_tag="VM910_23040"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65430.1"
                     /translation="GVRDVIAIDAVAKGAFTSGGVVVLADNSWAAMEGRRALKIVWDE
                     GPNASESSATLHKQFLENAAKPGKIVRNDGDADAVLAGSEKRVEATYEFPFAAHATME
                     PMNCTVLIRPDGAEAWVPTQAPQWAQDIIAGVSGLPRESVVVHTTLMGGGFGRRYQAD
                     FVMEAAQVAKATGKPVMVLWTREDDMQHDFYRPASYHRLQGAVGADGKLAAWKHFQTS
                     TSIAAMWEAKGEENPENSEFGTAVFIPYWTPNFRVEYTLAKSGVPRAWWRSVEHSTSG
                     FVVECFVDEMATAAGVDPYEYRLRLIGEDREIPDFANPKEGKPLNTARQKGVLKLAAE
                     KGAWGMPLPKGVARGIAGYYSFQSYTACVAEVSFKDKVVKVNRLVYAVDCGRPVNPAG
                     VRAQVESAAIYGLSATLHDAITIGGGRVEQANFNDYKMPRIADTPKTEVYVVGSNEEP
                     TGIGEPGLPVVGAAVSNALYALTGKRVRRLPIRPEDLA"
BASE COUNT          320 a          413 c          530 g          281 t
ORIGIN      
        1 ccggtgtgcg cgacgtgatt gcgatcgatg ccgtcgcgaa aggagcgttt acctccggag
       61 gtgttgtcgt actcgccgac aattcctggg cggcgatgga gggtcgccgg gcgctaaaga
      121 tcgtttggga cgaggggccc aacgcaagcg aatcgagcgc aacgctgcac aagcagtttc
      181 tggagaacgc cgcgaagcct gggaagatcg tgcggaatga cggtgacgcc gacgccgtgc
      241 tggccggttc cgagaaaagg gttgaggcca cctacgaatt cccgttcgct gcccacgcta
      301 cgatggagcc gatgaattgc accgttttga tccgcccgga tggcgcggaa gcgtgggttc
      361 cgacgcaggc gccgcagtgg gcccaggaca tcattgcggg cgtatcggga ttgccgcgcg
      421 aatctgtcgt tgtgcacacg acgctgatgg gcgggggatt cggaaggcgc taccaggccg
      481 atttcgtgat ggaagcggcg caggtcgcaa aagcaacggg caagcccgtg atggtgttgt
      541 ggacgcgcga agacgacatg caacacgact tttaccggcc cgcttcctat cacagattgc
      601 aaggcgcggt aggggccgac ggaaagcttg cggcgtggaa gcattttcag acctcaactt
      661 cgattgcggc gatgtgggaa gcaaagggcg aagagaaccc ggaaaactct gaatttggaa
      721 ccgcggtttt catcccgtat tggacgccaa attttcgagt ggaatacacg ttggcgaaat
      781 ccggggtgcc gcgagcgtgg tggcggtcag tggaacattc gacgagcgga ttcgtggtgg
      841 agtgtttcgt tgacgaaatg gccacggcgg cgggcgtgga tccctacgaa taccggctgc
      901 gtctgatcgg cgaggaccgc gaaatccccg atttcgcgaa cccgaaagag ggcaagccgc
      961 tcaataccgc tcggcaaaaa ggcgtgctca agctggcggc agaaaagggc gcctggggta
     1021 tgcctttgcc gaagggagtg gcgcgcggga ttgccgggta ttattcgttt caaagctaca
     1081 ccgcttgcgt tgcggaagtc agcttcaagg acaaggtcgt gaaagtaaat cggctcgtct
     1141 atgcggtgga ttgcggacgg ccggtaaatc ccgccggagt gcgcgcgcag gtggaaagcg
     1201 ccgcgattta cgggctctcg gcgacgctgc acgatgccat cacgatcggc ggtgggcgcg
     1261 tagaacaggc gaatttcaac gattacaaga tgccgcgcat cgcggacacc ccgaaaacag
     1321 aagtgtatgt ggtcggaagc aacgaagaac cgacgggaat cggcgagccc ggcctgccgg
     1381 tggtgggcgc tgcggtctcg aacgcgctct atgcgctgac gggaaagcgg gtccggcggt
     1441 tgccgatccg gcccgaagac ctggcctagc catcgcgttc gaattcgcgc aaaatggagc
     1501 gtgttcgttc ttccgctcat cctcgccggc acttccctgc tcat
//
LOCUS       DATLJT010001003         7944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3054489,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001003 DATLJT010000000
VERSION     DATLJT010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054489"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VM910_23045"
     CDS             <1..424
                     /locus_tag="VM910_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138004.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HVI65431.1"
                     /translation="TPTAKLRDSMQHVMQNNCAVFRTGEVLQEGQNLIHKVHNGISDI
                     SVSDRSLIWNSDLVETLEFANLIVQAVVTMDSAANRTESRGAHAREDFSARDDKNWMK
                     HTLAWIDAGGKTRIEYRPVHDYTMTNDVQYIPPKARVY"
     gene            547..1329
                     /locus_tag="VM910_23050"
     CDS             547..1329
                     /locus_tag="VM910_23050"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HVI65432.1"
                     /translation="MVEFALPKNSRITGGKTWPKPPGATETREFKVYRWNPDDGKNPS
                     TDTYYIDVNDCGPMVLDGLIWIKNNIDPTLTFRRSCREGVCGSCAMNIDGQNALACTK
                     SMHDVKDGPVKVNPLPHQPVVKDLVPDLTNFYAQYASIEPWLKTTSPTPQKEWRQSHE
                     DREKLDGLYECILCACCSTSCPSYWWNSERFLGPAALLQATRWVKDSRDEATGERLDN
                     LEDPFRLYRCHTILNCAKACPKGLNPSEAIAELKLKLVERQM"
     gene            1352..2278
                     /locus_tag="VM910_23055"
     CDS             1352..2278
                     /locus_tag="VM910_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI65433.1"
                     /translation="MSAPQIILHHFDQSPVSEKIRLIFGLKDIAWTSVRISRIMPRPD
                     LMPLTGGYRRTPVMQIGADVYCDTQCIMRELERRFPEPGLLPKGYEGLAWGSAMWTDR
                     SFFQNTVNLVFGSLADKVPADFIADREKLRGAKFDVAAMTAAIPQMRDQFRAHLQWID
                     AQLCDGRTFISGEAASLCDVNAYMNLWYVRAHLENADDMLAEFDHARAWAGRLRASGH
                     GPHAEISSAEALQIAHDATPQTEELHDPNDPNGRKPGDRVEVMPDDYGKVRVAGEIVA
                     LSAQHIAIRRHDPRAGEVVVHFPRAGFLIFPT"
     gene            2338..4332
                     /locus_tag="VM910_23060"
     CDS             2338..4332
                     /locus_tag="VM910_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HVI65434.1"
                     /translation="MPQANAKPQSRDGNQARDDAKSREDKAPIVEVVAEDGDHVEPPP
                     PDVVETDPELTPEEAEQARKDYLLTRFWISARGYWSHTGDKLAWPFSILLLLLIVGTV
                     GFQYGINVWNRAIFDAIEKRDSGTVYYLTAVFLPLAIGSVALGVAQVFARMAIQRRWR
                     TWLTTSVISRWLSNGRYYQLNLVDGDHKNPEYRIAEDLRIATDSPVDFIAGVTSAFLS
                     ASTFIVVLWTLGGALSLNLAGITVTVPGFLVVAAVLYAAIASGSITVIGRRFVQISED
                     KNQAEAEFRYALTRVRENGESIALLGGEQEERDGIDKTFANVLRQWARLAGQHMRTTL
                     VSQGSSLIAPVVPLLLSAPKFLDGSMTLGQVMQAASAFTIVQSAFGWLVDNYPRLADW
                     NACARRIASLMMSLDGLERAERAGGLGRIKRGETEGDAMLSLNDLSVSLDDGTAVVDE
                     TEVVIEPGERLLVAGESGSGKSTLVRAIAGLWPWGGGSVNFHPDRRLFMLPQRPYVPS
                     GTLRRAVAYPGAAEDWTLEEIGEALHKVGLAHLKDKIEEDAPWDQTLSGGEKQRLAFA
                     RLLLHNPDIVVLDESTSALDEKSQDRMMETVMRELPKATIISVAHRAELEAFHSRKVT
                     LERRKGGAKLVSDIDLIPRKGKRRLLGHFLRHRKATPKAA"
     gene            4423..5250
                     /locus_tag="VM910_23065"
     CDS             4423..5250
                     /locus_tag="VM910_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVI65435.1"
                     /translation="MMPDNDASPAPFGAFAPNAAQAAIVSLAHRSGLKRGAFRPWLSR
                     LINLVRDGPLDVSYQGASFRLYHQNSATERGALLNPDYNLEELDFLRAHTPTGGVFVD
                     IGANVGTYALVLARKVGTTGKVIAIEPHPVTHARLSFNRAASGLTQVMLVAAAAGPAD
                     GEVLIETDGDNLGASHVVSSDASAKAIRVPSFRLIRILEQAGAAKIDALKIDVEGFED
                     RVLTAFFKEAPQALWPRAVVIEHLSREEWLADCIADMLARGYDAAGKTRSNTLLVRS"
     gene            5278..5660
                     /locus_tag="VM910_23070"
                     /pseudo
     CDS             5278..5660
                     /locus_tag="VM910_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5843..7672
                     /locus_tag="VM910_23075"
     CDS             5843..7672
                     /locus_tag="VM910_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI65436.1"
                     /translation="MTRLRLVLLATTALSAMQFASSASHAQSAPQLMAQAPSEADKEK
                     EKKGPPPKAAPQPPPAPPKAAPPPPPAPPRAAPPPPPPQAPKAPPPPPPHPVAPPPPP
                     PPHPAAPPPPPAPTPHPTPPTPPAAAPAPHPSAPPPPPPPAAKQPPPPPPAAKQAPPP
                     PPPPPVGAPKQPPASPSPTPPVQPQGRPQPTPGTAPPPPAPGATPLPPPPGGGAGPQQ
                     FNRPITPGVTPPAGGTPPAGGGTPPTTTPGGQQGAAPPPAGTPPGAAATFTRRAPPQV
                     TAPIPAVPPPSQQALTPIPQGAPPQGPQRLEDFRAQRREVQEGGRTVITEPGRIIVRD
                     PSGMEFVRHDEMDRFRYGARDIRVQQLGGETRTVVIRPDGSEIITVVAPDGRLLRRIR
                     RDREGRELIIIDNTFRGPSLGFYVDLPPPIIRIPYNRYIVDASDAPPDLIYETMEAPP
                     VEQIERPYTLDEIRYSPGVRMLMPSIDVNTINFDTGSWEIAPDQAAKLQVIADGLNRA
                     ILRNPREVFLIEGHTDAVGNDTDNLSLSDRRAESAAELLTQQFGVPAENLTSQGYGAQ
                     YLKEQTAGPSRINRRVTIRRITPLLNGGQASLPPPPPGIAPPR"
     gene            complement(7741..>7944)
                     /locus_tag="VM910_23080"
     CDS             complement(7741..>7944)
                     /locus_tag="VM910_23080"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470518.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVI65437.1"
                     /translation="PNLTYQPERLSMEKVEDAAFTPVDRIGQLTMRNLDIADTRGKLD
                     LYAKTGLLSKGDGSYIPKLDGDK"
BASE COUNT         1497 a         2675 c         2452 g         1320 t
ORIGIN      
        1 cacgccgacc gcgaaactgc gcgacagcat gcagcatgtg atgcagaaca actgcgcggt
       61 gttccgcacc ggcgaggtgc tgcaggaagg gcagaacctg atccacaagg tgcacaacgg
      121 gatttcggac atttcggtgt cagaccgctc gctgatctgg aactccgacc tggtcgagac
      181 gctggagttc gcgaacctga tcgttcaggc ggtcgtcaca atggactcgg ccgcgaaccg
      241 gaccgagagc cgtggcgcgc atgcgcgcga ggatttcagc gcgcgcgacg acaagaactg
      301 gatgaagcac acgctggcgt ggatcgacgc tggcggcaag accaggatcg aataccgccc
      361 ggtgcacgat tacaccatga ccaacgacgt gcaatacatt ccgccgaagg cgcgcgtgta
      421 ctgataacac tgtcatgcgc gggcaaaagc gcaaggcgcg tcttcaagcg aacagcccgc
      481 gcatccgtct caaaaggatg gattgtcagg cccggcaatg acgagcgaag gataaaggcg
      541 agacagatgg ttgaattcgc acttccgaaa aactcgagga tcaccggagg caaaacctgg
      601 ccgaagccgc cgggtgccac cgagacgcgc gagttcaagg tctatcgctg gaatccggac
      661 gacgggaaga atccgagcac cgacacctat tacatcgacg tcaacgactg cggcccgatg
      721 gtgctcgatg gcctgatctg gatcaagaac aacatcgatc cgacgcttac cttccgccgc
      781 tcctgccgcg agggtgtctg cggctcctgc gcgatgaaca tcgacggcca gaacgcgctc
      841 gcctgcacca agtcgatgca cgacgtcaaa gacggcccgg tcaaggtcaa tccgctgccg
      901 catcagccgg tggtgaagga cctggttccc gacctcacca atttctatgc gcaatatgcc
      961 tcgatcgagc catggctgaa gaccacttcg ccgacgccgc aaaaggaatg gaggcagagc
     1021 cacgaggacc gcgagaagct cgacggactt tatgagtgca ttctttgcgc ctgctgctcg
     1081 acctcctgcc cgagctattg gtggaacagc gagcgcttcc tgggaccggc tgcgctgctg
     1141 caggccacgc gctgggtcaa ggattcgcgc gatgaagcca ccggcgaacg gctcgacaat
     1201 ctcgaggacc cgttccggct ctaccgctgc cataccatct tgaactgcgc caaggcgtgc
     1261 ccgaagggtc tcaacccgtc ggaagcaatc gctgagctca agctgaaact ggtcgaacgg
     1321 cagatgtgac ggcggccgga tcgatcgagc gatgtccgcg ccgcaaatca ttctgcacca
     1381 tttcgatcaa tcgccggtct ctgaaaaaat ccggctcatt ttcggactga aggatatcgc
     1441 gtggacctcg gtcaggatct cgcggatcat gccgcgcccc gacctgatgc cgctcaccgg
     1501 cggttatcgc cgcacgccgg tgatgcagat cggcgccgac gtctattgcg acacgcaatg
     1561 catcatgcgc gagctcgagc gccgctttcc cgagcctggt cttttgccga agggctatga
     1621 ggggctcgcc tggggcagtg cgatgtggac cgaccgctcc ttcttccaga acacggtcaa
     1681 cctggtgttc ggctcgctgg ccgacaaggt gccagccgac ttcatcgccg accgggagaa
     1741 gctgcgcggc gcgaaattcg acgtcgcggc gatgacggcg gcaatcccgc agatgcgcga
     1801 tcagtttcgc gcacacttgc aatggatcga cgctcaactt tgcgacgggc ggacctttat
     1861 cagcggagag gccgcaagcc tttgcgacgt caacgcctac atgaatctct ggtacgtgcg
     1921 cgcgcatctg gagaatgccg acgacatgct cgccgaattc gaccacgcgc gtgcctgggc
     1981 cgggcgcctg cgcgcgagcg gccacggacc gcatgccgag atatcgagcg ccgaggcgtt
     2041 gcagattgcc cacgacgcga cgccgcagac cgaggaattg cacgacccta acgacccgaa
     2101 cggccgcaag cccggcgaca gggtggaagt catgccggac gactacggca aggtgcgcgt
     2161 cgcgggcgag atcgtcgcac tctcggccca gcacatcgcc attcgccggc atgatccgcg
     2221 cgccggcgag gtggtcgtgc atttcccacg cgccggcttt ttgatctttc caacctaggt
     2281 cagcgccctg agcggaactt gccgggaacc ttctaatttc actggcgttg gcgtgtcatg
     2341 ccccaggcga acgccaagcc ccagtcgcgc gacggcaacc aggcccgcga cgacgccaaa
     2401 tcgcgcgaag acaaggcgcc gatcgtcgag gtcgtcgccg aagacggcga tcacgtcgaa
     2461 ccgccgcctc ccgatgtcgt cgagaccgat cccgaactca cgcccgagga ggcggagcag
     2521 gcgcgcaagg actacctgct gacccggttc tggatcagcg cccgcggcta ttggagccac
     2581 acaggcgaca agctcgcctg gcctttctcg attctgctgt tgctcctgat cgtcggtacg
     2641 gtcggtttcc aatacggcat caatgtctgg aatcgcgcaa tcttcgatgc catcgagaag
     2701 cgcgattccg gcacggtcta ttatctcacc gccgtgtttc ttccgctcgc aatcggcagt
     2761 gtcgcgctcg gcgtcgccca ggtgttcgcg cgcatggcga tccagcgccg ctggcgcacc
     2821 tggctgacca cttcggtgat ctcgcgctgg ctcagcaacg gccgctatta ccagctcaat
     2881 ctcgtcgacg gcgaccacaa gaatccggaa tatcgcatcg cggaggattt gcggatcgcg
     2941 accgactcgc cggtcgattt catcgcgggt gtcacgtccg cgttcctgtc ggcatcaacc
     3001 ttcatcgtcg tgctctggac cctcggcggc gcgctttcgt tgaacctcgc ggggataacg
     3061 gtgaccgttc cgggtttcct cgtcgtcgcc gccgtgcttt acgccgcgat cgcctcgggt
     3121 tcgatcaccg tcatcggccg ccgctttgtg cagatctcgg aggacaagaa ccaggccgag
     3181 gccgaattcc gctacgcgct gacgcgtgtg cgggaaaacg gcgaaagcat cgccctgctc
     3241 ggcggcgaac aggaggagcg cgacggcatc gacaagacgt ttgcgaatgt gttgcggcag
     3301 tgggcgcgac ttgcgggcca gcacatgcgc accacgcttg tgtcgcaggg ttcgagcctt
     3361 atcgctccgg tcgtgccatt acttttgagt gcgccgaaat ttctcgacgg cagcatgacg
     3421 ctcggacagg tgatgcaggc agcttccgcc ttcaccatcg tgcagagcgc gttcggatgg
     3481 ctggtcgaca attatccgcg tcttgccgat tggaacgcct gcgcgcgacg catcgcctcg
     3541 ctgatgatgt cgcttgacgg gctcgagcgt gccgagcgcg ccggcggact tggccggatc
     3601 aagcgcggcg aaaccgaggg cgatgcgatg ctcagcctga acgatctgtc ggtatcgctg
     3661 gacgacggca ctgccgtggt cgacgagacc gaggtcgtga tcgagccggg cgaacggctg
     3721 ctcgtcgccg gcgaatccgg atcgggcaag agcacgctgg tgcgggcaat tgccgggctg
     3781 tggccgtggg gcggcggcag cgtcaacttc catcccgaca ggcggctgtt catgctgccg
     3841 cagcggccct acgtaccctc gggcacgctg cgtcgcgcgg tcgcctatcc cggcgccgcc
     3901 gaggactgga cgctggaaga gatcggcgag gccctgcaca aggtgggact ggcccatctg
     3961 aaggacaaaa tcgaggagga cgcaccgtgg gatcagacct tgtcgggcgg cgaaaagcag
     4021 cggctcgcct tcgcgcggct cctcctgcat aatcccgaca tcgtcgtgtt ggacgagtcg
     4081 acgtccgcgc tcgacgagaa gagccaggac aggatgatgg agacggtgat gcgcgaattg
     4141 ccgaaggcca ccatcatcag cgtcgctcat agggcagagc ttgaggcgtt ccacagccgc
     4201 aaggtcacac tggaacgccg caagggtggc gcgaagctcg tgagcgacat cgatcttatc
     4261 ccgcgcaagg gcaagcgacg cctgctcggt catttcctgc gccatcgcaa ggcgacaccg
     4321 aaggccgcgt gatttgctcg tctcaatccc gttgcaaagt gccgttggag tcgcctggaa
     4381 aaccggctat gcatcccacc gcttcgtttt gaggatgccc ggttgatgcc cgacaatgat
     4441 gcttcgcccg caccattcgg cgcgtttgcg ccgaatgcgg cgcaagctgc gatcgtctcg
     4501 ctcgcccacc gttcggggct gaagcgcggc gcatttcgcc cgtggctgtc gcgtctcatc
     4561 aatctggtcc gcgacgggcc gctggacgtg tcctaccagg gggcctcgtt tcggctctac
     4621 caccagaact ccgccaccga gcgcggcgcc ctgttaaatc cggactacaa tctcgaggaa
     4681 ctggacttcc tgcgcgcaca tacgccgacc ggcggcgtgt tcgtcgatat cggcgccaat
     4741 gtcggcacct atgcgctggt gctggcgcgc aaggtcggca cgacgggaaa ggtcatcgcg
     4801 atcgagccgc acccggtcac ccatgcacgg ctctcgttca accgcgcggc gtcgggcttg
     4861 acgcaggtca tgctggtggc ggcagcggcc ggtcccgccg atggcgaggt tctgatcgag
     4921 accgatggcg acaatctcgg tgcgagccac gtggtgtcgt ctgacgcttc ggcaaaggcg
     4981 attcgggtgc cctcattccg cctgatacgg attctcgaac aggcgggcgc tgcgaaaatc
     5041 gatgcgctga agatcgacgt cgaaggcttc gaggatcgcg tgctcaccgc cttcttcaag
     5101 gaagcgccac aggctctgtg gccgcgcgcc gtcgtgatcg agcatctctc gcgcgaagag
     5161 tggctggccg attgcatcgc cgacatgttg gcgcgcggct acgatgccgc cggcaagacc
     5221 cgcagcaata cgctgctcgt gcgcagctag cttgctgtcc gatatctgga gggtgcgatg
     5281 attgatcata tcggatttcc ggtttccgat tacgagcgag cgaaggcctt ctatctgaaa
     5341 gcgttggcgc cgctcggcta cagccttgtg atggaagtga cgcaggagca gcagcccggc
     5401 gatccgcgcg ctgccggctt tggcgccaac ggcaagccgg atttctggat cggcggcgaa
     5461 ggcggattga acaagccgat gcatgtcgcg atccttgcga gcgatcgatc aacgcgttct
     5521 atcgcgcagc gctggcagca ggcgggcgtg acaatggtgc gcccggcatc cgcgcgcact
     5581 atcatccaaa ctattacggc gctttcgtac tcgatccgga cgggcacaat atcgaggcag
     5641 tttgccacac gctcgtctag gccgatccag ggaacttgaa accagcgcct gaatacgcaa
     5701 tgaacaggga ccgttccact gtcgcgcatc gaaaaataaa tccgaatccg cgttcgagcg
     5761 tcgccacaat ccggcctaac gcgtgattgt tcatcgccgc ggccagtatt caccagaaat
     5821 cattccggga ccaataatca tcatgaccag acttcgtctc gtgctgcttg cgacaaccgc
     5881 gctgtcggcg atgcagtttg caagctccgc atcacatgcg cagagcgcgc cgcagctcat
     5941 ggcgcaggct ccgagcgaag ccgacaagga aaaagagaag aaggggccgc cgccgaaggc
     6001 tgctccgcaa cctccgcctg ccccgccgaa ggccgcgcca cccccgccgc ccgcaccgcc
     6061 aagggccgcg ccgccaccac cgccgcctca agcgccgaag gctccgccac cgccaccccc
     6121 acatccggtt gcaccacccc cgcctccacc gccgcacccg gctgcgccac cgccgccacc
     6181 tgctccgacg ccgcacccta cgccgccgac gccgccggct gctgcgcccg caccgcatcc
     6241 ttcagcacca ccgccacccc cacctccggc agccaagcag ccaccaccgc cgccgccggc
     6301 agcgaagcag gctccgccgc caccgccacc gccaccagtt ggtgcaccga agcagccacc
     6361 ggcttcgccg agcccgacgc cgccggtgca gccacagggg cgccctcaac cgaccccggg
     6421 tacggcacct ccgccgccgg cacccggcgc gacgccccta ccgccgcctc ccggcggggg
     6481 cgccgggcca cagcagttca accgaccgat cactccgggc gtcacgcctc cggctggtgg
     6541 tactccgcct gcgggaggtg gcaccccgcc gacgacaact ccaggcggcc agcagggtgc
     6601 ggcaccgccg ccagccggca cgccgcccgg cgccgccgcc actttcacac gccgcgcgcc
     6661 gccacaggtg actgcgccaa ttccggccgt gccgccgccc agtcagcagg cgctgacgcc
     6721 gatcccgcag ggcgcgccgc cgcaaggccc gcaacgcctc gaggatttcc gtgcccaacg
     6781 gcgcgaggtc caggagggcg gccgcaccgt catcaccgaa cccggacgaa tcatcgtgcg
     6841 ggatccctca ggcatggagt tcgtccgcca cgacgagatg gaccgcttcc gctatggcgc
     6901 gcgcgacatc cgggtgcagc aactcggcgg cgagacccgc accgtggtga tccgtcccga
     6961 cggctcggag atcatcacgg tggttgcacc cgacggacgg ctgctgcgcc ggatcagacg
     7021 cgaccgcgag ggccgcgagc tcatcatcat cgacaacacc ttccgaggtc cctcgctcgg
     7081 gttttatgtc gacctgccgc cgccaatcat ccgcatcccc tacaaccgct acatcgtgga
     7141 tgcttccgac gccccgccgg acctgatcta cgaaacgatg gaagcgccgc cggtagagca
     7201 gatcgaacgg ccctatacgc tcgacgaaat ccgctacagt ccgggcgtgc ggatgctgat
     7261 gccgagcatc gacgtcaaca ccatcaactt cgacaccggc tcgtgggaga tcgcccccga
     7321 ccaggcggcg aagctgcaag tgatcgccga tggcctcaac cgcgcgattc tgcgtaaccc
     7381 gcgcgaggta ttcctgatcg agggccacac cgacgcggtc ggcaacgaca ccgacaatct
     7441 gtcgctgtcg gaccggcgcg cggaatccgc agccgaattg ctgacccagc agttcggggt
     7501 gcctgccgaa aacctgactt cgcagggcta cggcgcacaa tatctgaagg agcagaccgc
     7561 gggaccgagc cggatcaacc ggcgcgtcac catccgccgc atcacgccgc tgctcaatgg
     7621 tggacaggct tcactgccgc cgccgccgcc cggcatcgcc ccgccgcggt aagcgcgaaa
     7681 atcctcaatg caaatggccg ggagcgatcc cggccatttt tttcggctcg tcattgcgag
     7741 ctatttgtcg ccatcgagct tggggatgta agagccatcg cccttcgaca gcaagcctgt
     7801 cttagcgtag agatcgagct tgccgcgcgt atcggcgatg tcgaggttgc gcatcgtgag
     7861 ctggccgata cgatcgacgg gcgtgaacgc cgcatcttcc accttctcca tgctcagccg
     7921 ctccggctga taggtcaggt tggg
//
LOCUS       DATLJT010001004         6926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_298175,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001004 DATLJT010000000
VERSION     DATLJT010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298175"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VM910_23085"
     CDS             <1..1915
                     /locus_tag="VM910_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65438.1"
                     /translation="WKDNVFLMLNYESGFEAKAKGAPVVIRREIALARQAMVPMEGKA
                     VLARWDDRTDQLLVYASTQVPHIIRVGLAQFLGIDQGRVRVISPDVGGGFGYKCILQP
                     EELCVAWLALKYRQPFRYVEDRREHLVVGANSRQHQYRLTAHADERGRLLALDADITI
                     DGGAYSAWPFTVALEPGQATGNLPGPYDFRGYRCRTYCVATNKPGFLPYRGVARTGVC
                     FALELVIDAIARAVGREPWEVRHDNLVSAADMPYDNVARKHYDSGDYRKALMLAREKI
                     DLDAWRERQRRRERDGRLIGVGFASYCEQTAHGTSVFSGWGLPIIPGYDQATVRVTPD
                     GGLEVRAGVHSHGQGMETTLAQIAHEVTGIDIANIRVVLGDTGSTPYSTGTYASRSIV
                     MAGGAVANACKALIPRFLAIGAHLMQAAVVSMKYENGKIIGPRKSISLREVAEAWYLR
                     PQELPPDVDLGGLEATVGFRPKRDTGAFSYASHAAVVAVDTEAGAVEILDYVIVEDCG
                     TLVNPMVVEGQTLGGAAQGIGTALYEESPFDPNGQPLASTFADYLLPGPTEIPAIRIH
                     HMQTPSPYTEFGIKGMGEGGAIAPPAVIFNAVNDALRSLGVELTETPLTPRRLLEAIE
                     RTKSSTQPRVENS"
     gene            1912..2736
                     /locus_tag="VM910_23090"
     CDS             1912..2736
                     /locus_tag="VM910_23090"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI65439.1"
                     /translation="MKAARFDYARPADVGEALAALASAQGAKILAGGQSLGPMLNLRL
                     ARPALLVDISRLKALQQLEDTGRTWHIGAGVTHARLEDARGRLSGGEMLCEVAAAIAY
                     RSVRNRGTVGGSLAHADPAADWPLALAALGASVNIRGPTPRNILVAHFIQGAFTTTLA
                     ANEIIESIDVPKLSRAGRYGFYKFCRKTGEFAEASAAAVFDPESGAASIFLGALRGTP
                     QPLASLAQQVAREGKIAASTEAVSQALAQSVSGLDAVERSMATAVVTRALMQVLAP"
     gene            2754..3875
                     /locus_tag="VM910_23095"
     CDS             2754..3875
                     /locus_tag="VM910_23095"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI65440.1"
                     /translation="MNGRKIQALVEPRTHLADFLREHCRLTGTHLGCEHGVCGACTVL
                     IDDEPARSCLTYAVQCDGLAVQTIEGFDADPIMQELRTAFSREHGLQCGFCTPGMLIA
                     ARDIVRRLPDADARRIRVELSGNLCRCTGYLGIINAVESVIAARASKPTAVASAAAAP
                     PPAPLQAFAPTRKVAPASMTTPAPALEEARTGWTRFEESFVVARSPQTVWTIFADIPA
                     VVACLDGAELSEHDASTAKGKMTVKLGPIQAGFSGSAVIERDDRALRGIIRGAGSDRG
                     TGSRTKGEIAYRLEPEADGQKTRVSVTVEYSLQGALAQFSRSGLVLELGRRLVADFAA
                     NLNARLAGAQTRAAPRTPFNAGRFLSSWFVDRLRRPFKR"
     gene            3999..5675
                     /locus_tag="VM910_23100"
     CDS             3999..5675
                     /locus_tag="VM910_23100"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI65441.1"
                     /translation="MAWQRVASFGEIGVDGVLGVDVNGTPVALYRLSNEVFATSGICT
                     HALALLSDGFVEDGRIECPLHQGQFDIRSGKALCAPVTEDLRTFAVKLEGDDIFIDME
                     RPAASAPAVANAAANRKAEDGVRAAENGDQADIGTIAAVNGDPELSLTTRYVWPAEGL
                     TRIPDWVYTDQTIYEREIERIFHGRTWNYVALECEVPKAGDFIRSNVGPTPVVVVRAD
                     DGSINVVENRCSHRAAEFCRELSGNVKEFVCPYHQWSYDLEGNLAGVPFRRGVNGKGG
                     MPADFNNAQHGLRRLNVTTHRGVVFASYLHDMEPFQDYLGPEVLKEFEATFDGRKPRL
                     LGYYRHTLPGNWKLYHENLKDPYHATLLHTFLVTFGLLVAGNRSLMLADATGRHGVMA
                     SAKSERKGVSSDAKKEMRAYRDGMTLAEPRFMDFVEEFDSPWSVTMATIWPNLIIQRE
                     MNTLGVRQIIPTGPHEFIMKWTMFGFEGDDEEMTRHRLRQGNLMGPAGFLGLEDNEAI
                     KFVQDGMQHVPGGQHLVKLDPSVAAGTSDSLISEASIRAMYRHWRAEMGL"
     gene            5680..6219
                     /locus_tag="VM910_23105"
     CDS             5680..6219
                     /locus_tag="VM910_23105"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HVI65442.1"
                     /translation="MSEPAVTRHSSADGAPLESQLQAMLLHHEIESFNTRYAQALDEQ
                     RLSDWAEMFTDDALYVILSRENYDRGLPVGLIYCENKRMIHDRAFALMETSMFAPRYL
                     RHIIANQVVVGVESNGTIKSRANYVVLQVLFDRPDATLHQVGVYYDVFCRVGGELKLA
                     ERRCVYDNLLVPNALCIPV"
     gene            complement(6308..>6926)
                     /locus_tag="VM910_23110"
     CDS             complement(6308..>6926)
                     /locus_tag="VM910_23110"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HVI65443.1"
                     /translation="KKGQRVRITFQSIVDFVVFNRDNLQERFDQARTKANQGKIYLST
                     GDLLLSKLNNPMMAIVEDTFKGTHDLQYGMCSECSYTTRWNRRHLPPWNKLYPEAGIT
                     CREDLPLWGCYENIMTALQNYPIIPLDIPAPLNIGQHMELDEKGNLIWAVFAKHKELF
                     DPVPHIDLRAEMNCLCAASCDPGSGKLGSSKGFYPKPIRIQVFEE"
BASE COUNT         1279 a         2158 c         2190 g         1299 t
ORIGIN      
        1 gtggaaggac aacgtctttc tcatgctcaa ctacgagagc ggcttcgagg caaaagccaa
       61 gggcgcgccg gtcgtgatcc gacgcgagat cgcgcttgcg cgtcaggcca tggtgccgat
      121 ggaaggcaag gctgtgcttg cacgatggga tgaccgcacc gaccagctgc tcgtttatgc
      181 ctcgacccag gtcccacaca tcatccgcgt cggcttggcg cagtttctcg gcatcgacca
      241 ggggcgggtg cgcgtgatct cgccggatgt cggcgggggc tttggctaca aatgcatcct
      301 gcagccggaa gagctctgcg tggcgtggct ggcgctgaaa taccgacagc cgttccgcta
      361 cgtcgaagat cgccgcgagc acctggtggt cggcgccaac tcgcgacaac atcaataccg
      421 gctgactgcc catgccgacg aacgcggcag gttgttggcg ctcgatgcgg acatcacgat
      481 cgacggcggt gcgtattcgg cctggccgtt cactgtggcg ttggaaccag gacaggcgac
      541 cggcaacctg ccagggcctt acgatttccg cggctaccgc tgccgcacct attgcgtggc
      601 cacgaacaag cccggcttcc tgccctatcg cggcgtggct agaaccggcg tctgcttcgc
      661 cttggagcta gtgatcgacg ccatcgcgcg cgccgtcggt cgcgagccgt gggaggtgcg
      721 tcacgacaat ctcgtctcgg ccgccgacat gccttacgac aacgtggcgc gcaaacacta
      781 cgacagcggc gactatcgca aggcactcat gctcgcgcgc gaaaagatcg acctcgacgc
      841 gtggcgcgag cggcagcggc gcagagagcg cgatggccga ctgatcgggg tcgggtttgc
      901 ctcgtattgc gagcagaccg cacacggcac cagcgtattc tcgggctggg gcctgccgat
      961 cattcccggc tacgaccagg cgacggtgcg agtgaccccg gacggcgggc tcgaagtccg
     1021 cgccggcgtg cattcgcatg gccagggcat ggagaccacg ctcgctcaaa tcgcccacga
     1081 ggtcacgggc atcgacatcg ccaacatccg ggtcgtgctc ggcgataccg gcagcacgcc
     1141 ctactccacc ggcacttatg cctcgcgcag catcgtgatg gcgggcggcg cggtcgccaa
     1201 tgcgtgcaag gcgctcatcc cccgtttcct cgcgatcggt gcgcatctca tgcaggctgc
     1261 cgttgtctcg atgaaatacg agaacgggaa aatcatcggg ccgcgaaaat cgatctcact
     1321 gcgcgaggtc gccgaagcct ggtatttgcg gccgcaggag ctgccgcccg acgtcgacct
     1381 cggcgggctc gaggccaccg tcggctttcg tcccaaacgc gacacgggtg cgttcagcta
     1441 tgcctcgcat gccgcggtcg ttgccgtcga caccgaagcg ggcgcggtcg agattctcga
     1501 ttacgtcatc gtcgaggatt gcggcacgct ggtgaatcca atggtggtcg aaggccaaac
     1561 actcggagga gcagcccagg gcatcggtac cgcgctctac gaggagagcc cgttcgaccc
     1621 gaatggacag ccgcttgcct cgacctttgc cgattatctc ctgcccgggc ctaccgagat
     1681 tccagccatt cgcatccatc atatgcagac gccgtcgccc tacaccgagt tcggcatcaa
     1741 gggcatggga gaaggcggtg cgatcgcccc gcctgcggtg atcttcaacg cggtcaacga
     1801 tgccttgcgg tcgcttggcg tggaactgac cgagacgccg ctcaccccgc gccggctgct
     1861 cgaggcgatc gagcgcacga agtcgtcgac ccagccgcga gtggaaaact catgaaggct
     1921 gcgcgcttcg actatgcgcg ccccgccgac gtcggggagg cattagccgc cctggcgagc
     1981 gcgcaaggcg ccaagatttt ggcgggcggg cagtcgctgg gaccgatgtt gaatttgcgg
     2041 ctcgcgcgcc ctgccctact ggtcgacatc tcgcggctta aggcgcttca gcaactggaa
     2101 gataccggcc gcacgtggca catcggtgcc ggcgtcaccc acgcgcgtct ggaggatgcg
     2161 cgcggccggc tttccggcgg cgagatgctg tgcgaggtgg cggcggccat cgcctaccgt
     2221 tccgtgcgca accgcggaac tgtcggcggc agtctcgctc acgccgatcc ggcggcggat
     2281 tggccgctcg cgcttgccgc gctcggcgca tccgtcaaca ttcgcggtcc gaccccacgc
     2341 aacattttgg tcgcgcactt catccaagga gccttcacga cgacgcttgc ggcaaacgaa
     2401 atcatcgaat ccatcgatgt ccccaagttg tcgcgcgcgg gacgatacgg tttctacaaa
     2461 ttctgtcgca agacgggcga atttgcggaa gccagcgcgg ccgccgtctt tgatcccgaa
     2521 tccggcgccg cgagcatctt tctcggtgcg ctgcgcggaa caccccagcc gctcgcgtcc
     2581 ctggcgcagc aggtggcaag agaggggaag attgctgcct caaccgaagc cgtgagccag
     2641 gcgctcgcgc agtcggttag cgggctcgat gcggtcgagc gcagcatggc gacggctgtg
     2701 gtgacgcgcg ccttgatgca ggtgctcgcg ccgtgacgcc aatcgcgatt agcgtgaacg
     2761 ggcggaagat ccaggcgctg gtcgagccgc gcacccatct ggccgatttc ctgcgcgagc
     2821 attgccgcct gacgggaacc catctcggat gcgagcacgg cgtatgcggc gcatgcaccg
     2881 tgttgatcga tgatgagcct gcgcgctcct gcctcaccta cgcggttcag tgcgacggcc
     2941 ttgcggtgca aacgatcgag ggctttgacg ccgatccgat catgcaagag ctgcgcacgg
     3001 cattttcgcg tgagcatggc ctgcaatgcg gcttctgtac ccctggcatg ctgattgcgg
     3061 cgcgcgacat cgtgcgacgg cttccggacg cggatgcgcg ccgaatccgg gtcgagcttt
     3121 ccggcaatct gtgccgatgc acgggatatc tgggcatcat caacgcggtg gaaagcgtga
     3181 tcgcggcacg cgcgtcgaaa cccacggccg tcgcgtcggc ggctgctgcc ccgccgccgg
     3241 ctccgcttca ggccttcgcg ccgaccagga aagtcgcgcc cgcctcgatg accacgccag
     3301 cgcccgcgct cgaagaggca cgcacggggt ggacacggtt cgaggagagt tttgtggtgg
     3361 cgagatcgcc gcagacggta tggacgatct ttgcggatat tcctgctgtg gtcgcctgcc
     3421 tcgacggggc cgagctgagc gagcacgacg caagcaccgc gaagggaaag atgacggtaa
     3481 agctcggccc gatccaggcc ggcttttccg gctccgccgt gatcgaacgc gacgatcgag
     3541 cgctgcgcgg catcattcgc ggcgcaggca gcgacagggg caccggctca cggaccaagg
     3601 gtgaaatcgc ctatcggctt gagccggaag ccgacgggca gaaaacacga gtttccgtga
     3661 cggttgaata cagcttgcag ggcgcgctcg cccaattctc gcgttccggc ctcgtcctcg
     3721 agcttggccg ccgcctggtt gccgactttg ccgccaatct caatgcgcgg cttgcaggag
     3781 cacagactcg cgcagcacct cgcacgcctt tcaatgccgg gcgctttctc tcgtcgtggt
     3841 tcgttgatcg gttacgccgc ccgttcaagc gttagcaaga tatgatggta tgctgatcac
     3901 ctgaaagacc ttgccacgat tgcgcaaagt tcatggacct attggcctcg cgaaaaatga
     3961 aagctatgct gaaccatctc gtgtagaggc cgcagacgat ggcatggcag agggtcgcat
     4021 ccttcggcga aatcggtgtg gatggcgtgc tgggcgtaga cgtgaatggc acgcccgttg
     4081 cgctttatcg gttatcgaat gaggtctttg ccacttccgg aatttgcacg catgcgttgg
     4141 cattgttgag cgacggcttc gtcgaagacg gcaggatcga gtgcccgttg catcaaggcc
     4201 agttcgacat tcgctccgga aaggcgctgt gtgcgccggt gacagaggac ctgcgcacct
     4261 ttgccgtgaa gctcgaaggt gacgacatct tcattgatat ggagcgtccg gccgcctcgg
     4321 cgccggctgt ggcgaacgcg gcggcgaatc gcaaagcgga ggatggcgtt cgcgccgccg
     4381 agaacggcga tcaagcggac attggaacga ttgccgccgt gaacggcgat cctgagcttt
     4441 cgctgacgac acgttacgtt tggccggcgg aaggactcac gcgcatcccc gattgggttt
     4501 acaccgatca gacgatctac gagcgcgaga tcgagaggat cttccacggc cgcacctgga
     4561 actacgtcgc gctggaatgc gaagtgccca aggccggcga cttcatccgc tccaatgtcg
     4621 gcccgacgcc cgtggtggtc gtgcgtgccg acgacggctc aatcaatgtc gtcgagaacc
     4681 gctgttcaca tcgcgccgcg gagttctgcc gcgagctcag cggcaacgtc aaagaattcg
     4741 tctgccccta ccatcaatgg tcgtacgacc ttgaaggcaa cctcgccggc gttccatttc
     4801 gacgcggcgt caacggaaag ggcggcatgc ccgccgactt caacaacgcc cagcatggat
     4861 tgcgtcggct caatgttacg actcaccgcg gggtcgtgtt tgcttcttac ctccacgaca
     4921 tggagccgtt tcaggattac ctcgggccgg aggtgctcaa ggagttcgag gcgaccttcg
     4981 acggccgcaa gccaaggctg ctggggtact accgccacac gctgcccggc aactggaagc
     5041 tctatcacga gaatctcaag gacccctatc acgcgacgct gctgcacacc ttcctggtga
     5101 cgttcggctt gttggtcgcc ggcaaccgct cgctcatgct cgcggatgcg accggccggc
     5161 acggcgtcat ggcgtccgcc aagtcggagc gaaaaggcgt ctccagcgat gccaagaagg
     5221 aaatgcgcgc ttatcgcgac ggcatgacgc tggcggagcc gcgattcatg gacttcgtcg
     5281 aggagttcga cagtccctgg tcggttacga tggcgacaat ctggccgaat ctgatcatcc
     5341 agcgcgagat gaatacgctc ggcgtgcgcc agatcatacc gaccggtccg catgagttca
     5401 tcatgaagtg gaccatgttc ggcttcgaag gcgacgacga ggaaatgacc cgccatcgtc
     5461 tgcgccaggg caatctcatg ggcccggcgg gctttctcgg cctcgaggac aacgaggcga
     5521 tcaagttcgt ccaggacggg atgcagcacg ttccgggcgg gcagcatctg gtgaagctcg
     5581 atccctcggt tgcggcgggc acttccgaca gcttgatctc ggaagcctcg atccgcgcca
     5641 tgtaccgcca ttggcgcgcg gaaatgggac tctaaaagca tgagtgaacc cgccgtcacg
     5701 cgccattcct cggccgatgg cgcgccactc gagtcgcagc tgcaagcgat gctgctgcat
     5761 cacgagatcg aatccttcaa cacccgatac gcacaggcgc tcgacgagca gcggctatcg
     5821 gattgggcgg agatgttcac cgacgatgcg ctctatgtga tcttgtcgcg cgagaattac
     5881 gaccgcggat tgccggtcgg cctgatctat tgcgagaaca agcggatgat tcacgaccgc
     5941 gcgttcgcgc ttatggaaac ctcgatgttc gcgccgcgtt atttgcgaca catcatcgca
     6001 aaccaggttg tcgtgggcgt ggagagcaat ggaacgatca agtcgcgtgc gaactacgtc
     6061 gtgcttcagg tcctgttcga tcgccctgat gcgacgctgc atcaggtcgg cgtctactac
     6121 gatgtgttct gccgcgtggg tggcgagctc aagctcgccg aacgccgttg cgtctacgac
     6181 aatcttctcg tgccgaatgc cctgtgcatt ccggtgtagg gtgagaatct tcaccaaaat
     6241 agcgctcgcc gctgcaattg tcgtgagcgc cgtcgcgatc aacgccgcgt cgaagcgaca
     6301 agcatcttca ctcctcaaat acctggatcc ggatcggttt cggatagaac cccttggaac
     6361 tccccagctt gccgctaccg ggatcgcagc ttgcggcaca caaacaattc atttccgcgc
     6421 gcaaatcgat gtgcgggacg gggtcgaaca gctccttgtg cttggcgaac accgcccaga
     6481 tgagattgcc cttctcgtcg agctccatgt gctggccgat gttcagcggc gcagggatgt
     6541 cgagcggtat gatcggataa ttctgcagcg ccgtcatgat attttcgtag cagccccaca
     6601 acggcagatc ttcgcgacag gtgatgccgg cctccggata tagcttattc cacggcggca
     6661 ggtgccggcg gttccaacgg gtcgtgtacg aacattcgct gcacatgccg tattggagat
     6721 cgtgggtgcc tttgaaggta tcttcgacga tggccatcat cggattgttc agcttggaga
     6781 gcagcaggtc gcccgtgctc aaatagatct tgccctggtt tgccttggtc ctcgcctgat
     6841 cgaaacgctc ctggagattg tcgcggttga acaccacgaa atccacgatc gactggaagg
     6901 taatgcgaac gcgctggcct tttttc
//
LOCUS       DATLJT010001005         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2364641,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001005 DATLJT010000000
VERSION     DATLJT010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2364641"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..572
                     /locus_tag="VM910_23115"
     CDS             333..572
                     /locus_tag="VM910_23115"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HVI65444.1"
                     /translation="MQHWKRFGRVMQELSRLSDRKLADVGINRCDIDRNNRASRPQTT
                     TMRYHAAAFNLTLASKPSSRRAFSVERYPLGSAML"
     gene            complement(476..1204)
                     /locus_tag="VM910_23120"
     CDS             complement(476..1204)
                     /locus_tag="VM910_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI65445.1"
                     /translation="MKIFLAGATGAIGRRLVPLLLKAGHGVVGTTRSTTKADGLHAAG
                     VEPVVIDVFDAPALSTAVLEVHPDIVVHQLTDLPPGLDPSQMTEGTQRNARMRSQGTQ
                     NLVAAALGAGVRRLVAQSIAWIYAPGKEPHPEDDVLDIDADGTRAITVAGVATLERLT
                     MSSPPIDGVVLRYGHLYGPGTGTNAAEAPALHVDAAAWAAVLAIEKARRGIYNIAEPS
                     GYLSTEKARRELGFDANVRLNAAA"
     gene            complement(1287..1733)
                     /locus_tag="VM910_23125"
     CDS             complement(1287..1733)
                     /locus_tag="VM910_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65446.1"
                     /translation="MTEPILSRRHINHSALISLLAAAALDGTAAAQQHATSHVATRRD
                     VIRQELPGEPLRDISLIEVTYPPATGSPPHLHANGVIAFVVSGTIVSKVGDGAEQTFH
                     AGDAWWEPPGAIHRVSRNASSTEPATLLAIYVAPRSATADDLMKPI"
     gene            complement(1816..2292)
                     /locus_tag="VM910_23130"
     CDS             complement(1816..2292)
                     /locus_tag="VM910_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65447.1"
                     /translation="MNILTINLALSTLVFWIAAKLYVLPKLQDYEARAILLPILLLHS
                     LRHLGLMFLTSGATYPGIPPQFTHPAAYGDLVTAVLAMIAIPAVATRARSAKLLVWIF
                     SVVGSLDLIDAITLATVYDAAPFMGPAYWIPAFWVPALLVTHYLTFLVLLRGRWPA"
BASE COUNT          504 a          707 c          700 g          471 t
ORIGIN      
        1 gctgggcgga gccacgatag ccatggctca aaatggtccg cccactggag gatatccacc
       61 agctcggtgg aacccgaatt tctatagcca ctacggctac catcatcgct actacgccta
      121 ctatcctgtc taccccaccg tggctggcta ctgggactat tatcgcacta gctggccggg
      181 gcgaggtaac gacttggagt caactcgtta agcgccgccc tttgcttctg acttagcgcc
      241 ccaatcctct tccacctgcg ctcttcgact gagcgaggcg gcgggagggt ttgggagtgt
      301 cccttcaaac cgaagttctt tgaatccgct tcattcagca ttggaagcga tttgggcgcg
      361 tcatgcagga actttcccgg ttgagcgacc gtaaactggc cgacgttgga attaaccgct
      421 gcgacatcga ccgtaacaat cgcgccagta ggccccagac gactaccatg cgctatcacg
      481 cggcagcgtt caatctgacg ttggcatcga agccgagttc gcgccgtgct ttctccgttg
      541 agaggtatcc gctcggttcg gcgatgttgt agataccgcg acgcgccttc tcgatggcca
      601 gtactgccgc ccaggccgcg gcatcgacgt gcagcgccgg cgcctctgcc gcgttggttc
      661 cggttccggg gccgtagaga tgcccatagc gcaggacgac tccgtcgatc ggcggagagg
      721 acattgttag ccgttccagc gttgcgaccc ctgccacggt gatcgcgcgt gtcccgtcag
      781 cgtcgatatc gagcacatca tcctcgggat gcggttcctt ccccggcgca taaatccaag
      841 caatgctttg cgcgacgagc cgacgaactc ccgcaccgag tgccgcggcg accaggtttt
      901 gggttccctg actgcgcatg cgcgcatttc gctgcgttcc ttcggtcatc tggctgggat
      961 cgagacccgg cggcagatcg gtaagctgat ggacgacgat atccgggtga acctctaaaa
     1021 cagctgtgga aagcgctggc gcgtcgaaga cgtcgatcac aaccggctcc accccggcgg
     1081 cgtgcagtcc atctgccttg gtagtcgagc gggtcgtgcc gaccaccccg tgccccgctt
     1141 taagcagcag aggcaccagc cgccttccaa tcgcgcccgt tgctccggcc aagaaaattt
     1201 tcatgtcaac caccccacat ggaccttccc tgccaaaagc ttggttggga ccataagcgg
     1261 tcgaataaac gagcaaccaa gttagttcag atcggtttca tcaagtcgtc cgctgtcgca
     1321 ctcctgggcg cgacgtagat tgcgagcaga gtcgccggtt cggtcgagct cgcattacgc
     1381 gagacccgat ggattgcacc cggaggttcc caccaggcat cgccggcatg gaacgtctgc
     1441 tcggctccgt cgccgacctt cgagacgata gtgccggaaa cgacgaatgc tatgacgcca
     1501 ttggcgtgaa gatgcggtgg ggagcctgtt gccggcggat aggtcacttc gatcaagctg
     1561 atatctcgca gcggctcgcc agggagctcc tgccggatca cgtcgcgccg cgtcgcaacg
     1621 tgtgaggtcg cgtgttgttg cgcggcagcg gtgccgtcga gtgccgcggc tgcaagcaag
     1681 gaaatcaaag cactgtgatt gatatgtcgc ctgctcagaa taggttcggt catagcacac
     1741 tgtttccttt ggcccgccct tggccgcacg cgcaactcgc cgatagacgt aaagttcgtt
     1801 tcctgagata tccagctatg caggccaacg gccgcgaaga aggacaagaa acgtgagata
     1861 gtgagtcacc aacagcgcag gcacccaaaa cgcgggaatc caatatgcgg gtcccataaa
     1921 cggcgcggcg tcataaaccg tcgcgagcgt gatggcgtca ataaggtcga gcgaacctac
     1981 aacactaaaa atccatacca acagcttcgc gctgcgcgcc cgagtcgcga ccgccggaat
     2041 ggcgatcatt gctaggacgg ctgtaacaag atcgccgtag gcggccgggt gggtaaattg
     2101 tggcggaatg ccaggatagg tggctcccga tgtgagaaac atcaagccga ggtgccgcaa
     2161 ggagtggagc agcaggattg gcaataagat tgctctggcc tcgtagtcct ggagcttagg
     2221 caggacatag agtttagctg caatccaaaa gaccaatgtg ctgagcgcga ggttgattgt
     2281 caggatattc atggcacgcc tctaaggcga gatactgacg ctttaccccc gaaagcagaa
     2341 tcagatcacg cgcaatcaag accgctttgt gccaaaagcg ga
//
LOCUS       DATLJT010001006         3869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3054616,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001006 DATLJT010000000
VERSION     DATLJT010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054616"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /gene="sdhA"
                     /locus_tag="VM910_23135"
     CDS             complement(<1..1544)
                     /gene="sdhA"
                     /locus_tag="VM910_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HVI65448.1"
                     /translation="MASDGNGKATNGGTPGGNGKAYPIEDHTYDVVVVGAGGAGLRAV
                     VGCSEAGLRTACITKVFPTRSHTVAAQGGISAALGNMHPDDWRWHMYDTVKGSDWLGD
                     QDAIEYMVRNAPEAVYELEHWGVPFSRTEDGKIYQRPFGGMTLDYGKGQAQRTCAAAD
                     RTGHAMLHTMYGQALRHSAEFFIEFFAIDLIMDDQGACRGVIALKLDDGTLHRFRAQT
                     TILATGGYGRAYASCTSAHTCTGDGGGMALRAGLPLQDMEFVQFHPTGIYGAGCLVTE
                     GARGEGGYLVNSEGERFMERYAPSAKDLASRDVVSRAMTIEIREGRGVGKKKDHIFLH
                     LDHLDPKVLQERLPGISESARIFANVDVTREPIPIVPTVHYNMGGIATNYHAEVLTKK
                     DGDDNAIVAGLMAIGEAACVSVHGANRLGSNSLIDLVVFGRAAALRCAEKLAAHAKQP
                     ELPKDSADQALGRLDHFRHASGGTPTAKLRDSMQHVMQNNCAVFRTGEVLQEGQNLIH
                     KVHNGIS"
     gene            complement(1548..1955)
                     /gene="sdhD"
                     /locus_tag="VM910_23140"
     CDS             complement(1548..1955)
                     /gene="sdhD"
                     /locus_tag="VM910_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HVI65449.1"
                     /translation="MSAHDTHRSPSMRTPLGRVRNLGASHSGTADFWRQRLTAVAMTL
                     LIVPVLVVVMMLLGRNQAGAAQILGSLPIAVILLLFIVASTWHMKIGMQVVIEDYVHN
                     EKLKLASIIANNFFSFAVALAAIYAILKLSSGV"
     gene            complement(1952..2350)
                     /gene="sdhC"
                     /locus_tag="VM910_23145"
     CDS             complement(1952..2350)
                     /gene="sdhC"
                     /locus_tag="VM910_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HVI65450.1"
                     /translation="MTARIERPLSPHLQTYRWTLTMVMSIVHRLTGVALYFGTLLLAW
                     WLIAAASGPTAYAHVQAFTSSFIGRLIVFGYTWALLHHLLSGIRHFVWDLGYGFKANE
                     REALTWGALIGGLSLTVLVWIAAYVIGGGR"
     gene            2603..3466
                     /locus_tag="VM910_23150"
     CDS             2603..3466
                     /locus_tag="VM910_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI65451.1"
                     /translation="MTIAGLDLSQLVELALLLIAVGALAGFLAGVFGIGGGAVLVPVF
                     YECFRLAGVPLEVRMPLCVGTSLAVIIPTSIRSFRAHYLRGAVDMEILKEWWIAILLG
                     VLAGSVTARYAPERLFKYVFVCVAWSATLRLWLGRESWKLGDEMPKGFLMKVYGFGVG
                     ILATLMGVGGGLFSNLLMTFYGRPIHQAVATSSALAVLVSIPGALGYIYAGWPVAARF
                     PNVAALQAPLALGYVSLIGAVLVMPTSLWTAPLGVKAAHAMSKRTLEIAFGCYLFIVG
                     GRFVISLINGQ"
     gene            complement(3506..>3869)
                     /locus_tag="VM910_23155"
     CDS             complement(3506..>3869)
                     /locus_tag="VM910_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598782.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HVI65452.1"
                     /translation="IDDKGYVHILGRGKDLVISGGFNVYPKEIESEIDAMPGVVESAV
                     IGVPHADFGEGVTAVVVCHPGTGVDEATVLRGLEGRLAKFKMPKRVFVVDELPRNTMG
                     KVQKNILRDNYKDIYSKK"
BASE COUNT          664 a         1216 c         1236 g          753 t
ORIGIN      
        1 gaaatcccgt tgtgcacctt gtggatcagg ttctgccctt cctgcagcac ctcgccggtg
       61 cggaacaccg cgcagttgtt ctgcatcaca tgctgcatgc tgtcgcgcag tttcgcggtc
      121 ggcgtgccgc cggaggcatg gcggaaatga tcgagccggc cgagcgcctg gtccgcggaa
      181 tccttcggca gctccggctg cttggcgtgg gcggcaagtt tttccgcgca gcgcaacgcg
      241 gccgcgcgac cgaacaccac gagatcgatc agcgaattcg agccgagacg attggcacca
      301 tgcacggaca cgcaggccgc ctctccgatc gccatcaggc cggcgacgat cgcattatcg
      361 tcgccgtcct ttttggtcag tacttcggca tgataattgg tggcgatgcc gcccatgttg
      421 tagtgcaccg tcggcacgat cggaatcggc tcccgtgtga cgtcgacatt ggcgaagatg
      481 cgcgccgatt cggaaatgcc gggcagccgc tcttgcagca ccttgggatc gagatggtcg
      541 aggtgcagga agatgtgatc ctttttcttg ccgaccccgc ggccttcgcg gatctcgatg
      601 gtcatggcgc gcgacaccac gtcgcgcgag gcaaggtcct tggccgaggg cgcgtaacgc
      661 tccatgaagc gttcgccttc ggagttgacg agatagccgc cctcgccgcg cgcgccctcg
      721 gtcacgagac agcccgcgcc gtagatcccg gtcggatgga actgcacgaa ctccatgtcc
      781 tgcagcggga ggcctgcgcg cagcgccatg ccgccgccgt cgccggtgca ggtatgcgcc
      841 gaggtgcagg aggcgtaggc gcggccatag ccgccggtcg cgaggatcgt ggtctgcgcg
      901 cggaaacggt gcagcgtgcc gtcgtcgagc ttcagtgcga tgacgccgcg gcaggcgccc
      961 tggtcgtcca tgatcaggtc aatcgcgaaa aactcgatga agaattccgc cgaatggcgc
     1021 agcgcctggc cgtacatcgt gtgcagcatg gcgtggcccg tgcggtcggc agcggcacag
     1081 gtgcgctgcg cctgaccctt gccgtagtcc agggtcatgc cgccgaacgg ccgctggtag
     1141 atcttgccgt cttcggtgcg cgagaacggc acgccccagt gttcgagctc gtagacggct
     1201 tcgggggcgt tgcgcaccat gtactcgatc gcatcctggt cgccgagcca gtccgatccc
     1261 ttgacggtgt cgtacatgtg ccagcgccag tcgtcgggat gcatgttgcc gagcgcggcc
     1321 gaaatgccgc cctgggctgc gaccgtgtgc gagcgcgtcg gaaatacttt ggtgatacag
     1381 gcggtgcgca gtcccgcctc gctgcagccc accacggcgc gcaggcctgc gccgccggcg
     1441 cccaccacca cgacgtcgta agtgtgatcc tcgatcgggt aggcctttcc gttgccgccg
     1501 ggagtgccgc cgttcgtggc cttgccgttg ccgtcgctcg ccatgggtca gactcccgat
     1561 gacagtttga ggatggcgta gattgcggcc agcgccaccg cgaaagagaa gaaattgttg
     1621 gctatgatcg aggcgagctt cagcttctcg ttgtgaacgt agtcctcgat cacgacctgc
     1681 atgccgatct tcatgtgcca ggtgctggcg acgatgaaga gcaagaggat gaccgcaatc
     1741 ggcagcgaac cgaggatttg cgccgcgccc gcctggttgc ggccgagcag catcatcacg
     1801 accaccagca ccggaacgat cagcagcgtc atcgccacgg cagtcagccg ctggcgccag
     1861 aaatcggcgg tgcccgagtg cgaggctccg aggttgcgga cccggccgag cggggtgcgc
     1921 atcgagggcg agcggtgggt gtcatgtgcg ctcatcgtcc gcccccgatc acataggccg
     1981 cgatccacac cagcacggtc agcgagaggc ccccgatcag cgcgccccag gtcagcgctt
     2041 cccgctcatt ggccttgaag ccgtagccca ggtcccaaac gaaatgcctg atgccgctga
     2101 gcagatgatg caatagcgcc caagtataac cgaacacgat cagccgcccg atgaaactcg
     2161 atgtgaaggc ctggacgtgg gcgtaggcgg ttggtccgga cgccgccgcg atcagccacc
     2221 atgcgaggag cagcgtgccg aaatacagcg cgaccccggt gaggcgatgg acgatcgaca
     2281 tcaccatcgt caacgtccag cgataggttt gcaaatgcgg cgaaagcggt cgttcgatcc
     2341 tggctgtcat ggggcggctt gacgatgttg cggacccagt tgtgagaccc gcctttgggc
     2401 ccatcgggat cacggtggat tgagctttat ttacgaagtc gaaccagaca gcgcaacgcc
     2461 gaatcgcggc aattccgaac cagtattcat acctacaggc gagacttgca atcgcacacg
     2521 ttgacaaacg gcaacgcaat ctgctggctg gtatacctga agcacgattc aagaacgact
     2581 tgttcgggac caggaaacgg ccatgactat cgcggggctc gacctctccc aacttgtcga
     2641 actggcactg ctgctgattg cggtgggtgc tctggctgga tttctggccg gtgtcttcgg
     2701 gatcggcggc ggcgccgtgc tggtgccggt gttctacgaa tgctttcgcc ttgccggcgt
     2761 gccgctcgag gtgcggatgc cgctctgtgt cggcacctcg cttgccgtga tcatcccgac
     2821 ctcgatccgc tcgtttcggg cccattacct caggggcgcg gtcgacatgg agatcttgaa
     2881 agagtggtgg atcgcgatcc tgctcggcgt cctcgccggc agcgtcaccg cgcgctatgc
     2941 gccggagcgg ctgttcaaat acgtgtttgt ctgcgtggcg tggtcggcga cattgcggct
     3001 ttggctagga cgcgagagct ggaagctcgg cgatgagatg ccgaagggat ttttgatgaa
     3061 ggtctacggc ttcggcgtcg gtatcctcgc caccttgatg ggtgtcgggg gcggcctgtt
     3121 ctccaatctc ctgatgacgt tctatggccg ccccattcat caagcggtag cgacgtcgtc
     3181 ggcgctcgcc gtgctggtct cgatccccgg cgcgcttggc tacatctatg ccggctggcc
     3241 ggtcgcggcg cgcttcccga atgttgctgc gctgcaggcg ccgctcgcgc tcggctacgt
     3301 ctcgctgatc ggtgccgtgc tggtgatgcc gacgagcctc tggaccgcgc ccctcggcgt
     3361 taaagccgcg cacgcgatgt caaagcgcac gctcgagatc gcgttcggct gctatctctt
     3421 catcgtcggt ggccggttcg tgataagcct gatcaacgga caatagttgc cagggacaaa
     3481 taagttcgac gcgatcgcga cgcccttatt tcttgctgta gatgtccttg taattgtcgc
     3541 gcaaaatatt cttctgcacc ttgcccatgg tgttgcgcgg caattcatcg accacgaaca
     3601 cccgtttcgg catcttgaat ttggcaagcc gcccctcgag ccctctcagc acggtcgcct
     3661 cgtcgacccc ggtgccggga tggcagacca ccaccgcagt cacgccctcg ccgaaatcgg
     3721 catgcggtac gccgatcacg gccgattcca cgacgccggg catcgcgtcg atctcgctct
     3781 cgatttcctt cggatacaca ttgaagccgc cggagatcac gaggtccttg ccgcggccga
     3841 gaatgtgcac ataacccttg tcgtcgatc
//
LOCUS       DATLJT010001007         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_67885,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001007 DATLJT010000000
VERSION     DATLJT010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67885"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VM910_23160"
     CDS             complement(<1..748)
                     /locus_tag="VM910_23160"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI65453.1"
                     /translation="MATEDVDPRFADLDAWALASAMEAMWEGQLAAVAAIGRALPAIT
                     AATTAAEQALGDRGRLIYVGAGTSARVAVQDGAELTPTFAWPQERLRFIIAGGESAFA
                     ASVEGAEDDAADAVKQIDEAGLSTRDVVIAVSASGTTPFTVAALQQARAHHAVTIGVA
                     NNAGTPLLAAAKYPVLVETGRELIAGSTRMKAGTAQKAVLNLISTGIMLRLGRVYRGM
                     MVNMPPTNAKLKRRAESMVARIAGCSEAKAA"
     gene            complement(801..1700)
                     /locus_tag="VM910_23165"
     CDS             complement(801..1700)
                     /locus_tag="VM910_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVI65454.1"
                     /translation="MVTRGTLYLGVDGGGSRCRARLEDERGAVLGEGLSGPATTRLGV
                     DKAWRSIMRACTAAAEHAALERADFARMHAGIALAGLGRRGAEAALNEIAHPFASVRF
                     ISDGLAACLGAHSGANGAIVVAGTGSIGVGLINGRELRFGGYGFPISDEGSGADIGLQ
                     VIRRALRAADGRGETSPLLQEVLGAFDDDAYQAVAWSEQATATDYAAFAPMVLRHAMQ
                     GDPIGRRIVERAADAIGDLLDLFLRQGIDQLSLVGGLSDAIVPWLTPDLRARLKPPKG
                     DAVAGALLVARRRFDVAEAPVER"
     gene            1848..3008
                     /gene="nagA"
                     /locus_tag="VM910_23170"
     CDS             1848..3008
                     /gene="nagA"
                     /locus_tag="VM910_23170"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HVI65455.1"
                     /translation="MDTTRFPAQFTIAALQLFDGTVMQGPALVTVSDGLIESVRPGDA
                     AALGGITLPDDAILAPGFIDIQVNGGGGVLLNDQPTAAGIRVIVDAHRKSGTTGCLPT
                     LITDRRDVLERLVEVAPAALTIPGVLGFHLEGPALNKARKGIHLESGIRVPDARDMAA
                     LKSFGHCGRSIVTLAPECVEASVIDDLIASGLRISIGHSDATARQVAQAADRGVTGVT
                     HLFNAMSQLTAREPGVVGAALCDDRLFAGIICDGLHVDPASLRIAIQCKGYDRLMLVT
                     DAMPLVGTDQRDFLLQGRRISLQAGRLTGPDGTLAGAHLTMIEAVRNAVTMLGVELAD
                     ALTMASRTPASFLGLESQLGSIAPFYRADLVAFSSRFEVIETWIAGQPLEST"
BASE COUNT          511 a         1017 c          975 g          548 t
ORIGIN      
        1 cggcagcctt cgcttcgctg catccggcga tccgggcaac catgctctcc gcacgccgtt
       61 tcagcttggc gttggtcggc ggcatgttca ccatcatgcc gcgatagacg cggccgaggc
      121 gcagcatgat gccggtcgat atcaggttga gcacggcctt ttgcgcggtg cccgccttca
      181 tccgcgtcga gccggcgatc agctcgcgcc cggtctcaac cagcaccgga tatttcgctg
      241 cggcaagcag cggcgtcccc gcattgttgg caacgccgat cgtcacggcg tgatgcgcgc
      301 gcgcctgctg cagcgccgcg acggtgaacg gcgtcgtgcc gctggcggag actgcgatca
      361 ccacgtcgcg cgtggagagg cctgcttcat cgatctgctt gactgcgtcg gcggcatcgt
      421 cttcggcccc ttcgacgctg gcggcaaagg cgctctcgcc gcccgcgatg atgaagcgca
      481 gccgctcttg cggccaggcg aaggtcggcg tcagctcggc gccgtcctgc acggcgacgc
      541 gggccgaggt gccggctccg acatagatca gacgtccgcg atcgccgagc gcctgctctg
      601 ccgccgttgt cgccgcggtg atggcgggca gcgcgcgccc gatggcggcg accgcggcga
      661 gctggccctc ccacatcgct tccatcgccg aggccaatgc ccaggcgtca agatcggcaa
      721 agcgagggtc gacgtcttcg gtagccatct atgtgatccg cctgtctcac ggatttgcaa
      781 cgcagaaccc ggaaaccagt tcacctctca accggcgcct cggcaacatc gaaacgccgc
      841 cgtgccacca gcagcgcgcc ggcgaccgca tcgcccttgg gcggtttgag ccgggcgcgc
      901 agatcgggcg tcaaccaggg aacgatcgca tcggataatc ccccgaccag cgacaactgg
      961 tcaattccct gccgcaagaa caaatcgagg agatcgccga tcgcgtcggc tgcgcgttcg
     1021 acgatgcgcc ggccgatcgg atcgccctgc atggcgtgcc gcagcaccat cggggcgaac
     1081 gccgcataat cggtcgccgt cgcctgctcg gaccaggcga cggcctgata ggcgtcatcg
     1141 tcgaacgccc cgagcacttc ctggagcaac ggagatgttt cgccgcggcc atccgctgca
     1201 cggagcgcgc gcctgatcac ctgcaatccg atgtcggcac cgcttccctc atcagagatc
     1261 ggaaatccat atccgccgaa gcggagctcg cgaccattga tcaggccgac gccgatcgac
     1321 cccgtgccgg cgacgacgat ggcgccattc gctccgctgt gggcgccgag acaggcggcc
     1381 agcccgtcgc tgatgaagcg taccgacgca aacggatgtg caatctcgtt gagtgccgct
     1441 tccgcgccgc ggcgtccaag acccgcaaga gcgatcccgg cgtgcatccg cgcaaaatct
     1501 gcgcgctcca gcgctgcgtg ctcggccgcg gccgtgcacg cccgcatgat cgaacgccac
     1561 gccttgtcga ctccaagccg cgtcgttgcc ggccccgaca agccctcgcc gagcaccgcg
     1621 ccacgttcat cctccaggcg agcgcggcag cggctgccgc cgccgtcaac cccgaggtat
     1681 agtgtgcctc tggtcaccat ggcgttccaa cgccgagacc ttcgttccgg ggacctgtcc
     1741 ctgtcaagcg ggcaggacgc aggggggacg catgtgatct cagtccgtgc ggctgtgttt
     1801 tttcagtgct aaattcaaat gcctgacaaa tacgcaatca acatatcatg gacacaacca
     1861 ggttcccggc acaatttacg attgctgcgc tgcaattgtt cgacggaacg gttatgcaag
     1921 gaccggcact ggttacggtg tcggacggcc tgatcgaaag tgtcagaccc ggagacgcgg
     1981 ctgcgctcgg cggtatcacg ctgccggacg atgcgattct cgcgcccggt tttatcgata
     2041 tccaggtcaa tggtggcggc ggtgtgcttc tcaacgatca gccgaccgcg gccggaatcc
     2101 gcgtcattgt cgacgctcat cgcaagagcg gcaccaccgg gtgcctgccg acgctgatca
     2161 ccgatcgcag agacgtgttg gagcgcctcg tggaggtcgc gccggccgct ctgacaattc
     2221 caggcgtgct cggcttccac ctggagggtc ctgcgctcaa caaggcacgc aagggcattc
     2281 atctggagtc cgggattcgc gtgccggacg cgcgagacat ggccgcattg aagagttttg
     2341 gtcattgcgg ccgctcgatc gtcacgctgg ctccggagtg cgtcgaggcg tcggtgatcg
     2401 acgatctcat agcaagcggc ttgcgcatct cgatcggtca tagcgatgcg actgcaagac
     2461 aggttgcgca ggcggcggat cgcggcgtga ccggcgttac tcacttgttc aacgccatgt
     2521 cgcagttgac cgcccgggag ccgggcgttg tcggggctgc gctctgcgac gaccggctgt
     2581 ttgccggaat catttgcgat ggcctgcacg tggatcccgc ctccttgcgg atcgcgattc
     2641 aatgcaaggg gtacgatcgg cttatgcttg tcaccgacgc gatgccgctg gtcggaacgg
     2701 atcagcggga ttttttgctg caggggcgac gaatatcgct gcaggcgggt cgcttgactg
     2761 gtcccgatgg cacgcttgcc ggcgcgcatc tcaccatgat cgaggcggtg cgcaatgcgg
     2821 tcaccatgct cggcgtcgag cttgccgatg cgctgacgat ggcgtcgcgc acgccggcaa
     2881 gcttcctggg ccttgagtcg caactgggct cgatcgcgcc cttctatcgc gccgatcttg
     2941 ttgccttcag cagccggttc gaagtcatcg agacgtggat cgccggccaa ccgctggagt
     3001 cgacgtgata ttgaagggac ccgtcacgac tccccgggac tgagcatctc t
//
LOCUS       DATLJT010001008         1692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_67895,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001008 DATLJT010000000
VERSION     DATLJT010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67895"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..>1692)
                     /locus_tag="VM910_23175"
     CDS             complement(79..>1692)
                     /locus_tag="VM910_23175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI65456.1"
                     /translation="VPISAVLGRLVETKQMIHVADLATQERYATSAIVKLAGARSFVG
                     VPMLKEGRLIGAIAIYRQEVRPFTDKQVDLLTNFARQVVIAIENTRLLNELRQSLQQQ
                     TATADVLKVISRSTFDLVTVLETLVASAARLCDADKGFIARREGVGYRLAANFGFTPD
                     FVEYVGQVLIEPGRNTLVGRTALEGRTIHIPDIRTDPEYTWSEAIERSGGVRTILGVP
                     LMREGVPMGVFNLARVTVRPFTEKQIELVSTFADQAVIAIENVRLFDEIQDKSRQLEL
                     ASQHKSQFLASMSHELRTPLNAIIGLTEMMVTNAARFGTEKAAEPLRRVHRAGTHLLG
                     LINQVLDLSKIEAGKLELNPEAVNLAPLIDEVVGTARQLAEQNKNRLVVETQEKLGSL
                     TVDPMRLRQILLNLLSNACKFTTQGEVRLQVGKVVDGRNWIEFAVADTGIGMTPEQQA
                     KLFAEFTQADSSTAQRYGGTGLGLAITRKLARMMGGDVTVASEPGKGSVFTVRLPGRV
                     GTPARSSSEDIKPQAGDASVRDSGMEAAS"
BASE COUNT          280 a          527 c          541 g          344 t
ORIGIN      
        1 cgaaatcgcc cagcagctcg agccgcatct tgagcatgta gacgttgtcg tcgttgtcct
       61 cgacgtagag tactttggtc acgacgctgc ttccatcccg ctgtcgcgca cgctggcatc
      121 gccagcttgc ggtttgatgt cctcgctcga gcttcttgca ggggtaccga cgcggccggg
      181 caggcggacg gtgaagaccg agcccttgcc cggttcgctc gccaccgtca cgtcgccgcc
      241 catcatgcgc gcgagcttgc gcgtgatcgc aaggccgaga ccggtgccgc cgtagcgttg
      301 cgcggtcgat gagtcggctt gcgtgaactc cgcaaacagt ttggcctgct gctcgggcgt
      361 catgccgata ccggtatcgg ccaccgcgaa ctcgatccag ttgcgtccgt ccaccacctt
      421 gcccacctgc agcctgacct cgccctgcgt ggtgaacttg caagcattgc tcaacaggtt
      481 gagcaggatc tgccgcagcc gcatgggatc gacggtcagc gacccgagtt tctcctgcgt
      541 ttcgaccacc aggcggttct tgttctgctc ggcgagctgg cgcgcggtgc cgacgacctc
      601 gtcgatcagc ggtgccagat tcaccgcttc ggggttgagc tcgagcttcc cggcttcgat
      661 cttcgagaga tcgaggactt ggttgatgag gccgaggagg tgggtgccgg cgcgatgcac
      721 ccgccgcagc ggctccgcgg ctttttcggt gccgaagcgc gccgcgttgg tgaccatcat
      781 ctcggtcaag ccgatgatgg cgttgagcgg ggtgcgcagc tcgtggctca tgctggcgag
      841 gaactgcgat ttgtgctggc tcgccagttc gagctggcgg ctcttgtcct ggatttcgtc
      901 gaacagccgc acgttctcga tggcgatcac cgcctgatcg gcgaatgtcg aaacgagttc
      961 gatctgcttc tcggtgaacg gacgcacggt cactctcgcc aaattgaaca cgcccatagg
     1021 gacgccttcc cgcatcagcg gtactcccag gatggtgcga acgcctccac tgcgctcgat
     1081 tgcctcggac caggtatatt cgggatcagt gcggatgtcg ggaatatgaa tagttcgccc
     1141 ctcaagcgcc gtgcgcccga ccagcgtgtt gcggccgggt tcgattaaaa cctgtcccac
     1201 gtactccacg aaatcgggcg taaatccgaa gttcgccgca aggcgatagc ccactccttc
     1261 ccggcgggcg atgaagccct tatccgcatc gcacagacgg gcggccgacg cgaccagcgt
     1321 ttcgagcacc gtaacaagat cgaacgtcga gcggctgatg actttgagca catcggcagt
     1381 ggcggtctgc tgctgcagcg attgccgcag ctcgttgagc agcctggtat tctcgatggc
     1441 gatgacgacc tggcgcgcga aattggtgag caggtcgacc tgcttgtcgg tgaacgggcg
     1501 cacctcctgg cggtaaatgg cgatcgcgcc gatcagcctg ccctccttga gcatcggtac
     1561 gccgacgaac gagcgcgcgc ccgcgagttt gacgatggcg gaggttgcgt agcgctcttg
     1621 cgtcgcgagg tcggcgacgt ggatcatctg ttttgtttcg accaggcggc ccagaacggc
     1681 cgagatgggg ac
//
LOCUS       DATLJT010001009         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1676453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001009 DATLJT010000000
VERSION     DATLJT010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1916
                     /locus_tag="VM910_23180"
     CDS             171..1916
                     /locus_tag="VM910_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HVI65457.1"
                     /translation="MTRDDDLYVRLGRVRDGGRGSGRRVKPFIAQALIATEKAGGLHR
                     RSGRNSRRTAFGRAGAASVAASQLISDRARHVTVKARVVRHRARGAPLRTHLAYLRRD
                     GVTRDGVAGRMFNAERDDADHRAFAERCDGDRHHFRFIVSPEDAEQLSDLKSFTRELM
                     RQAERDLGTRLDWIGVDHWNTNNPHVHVIVRGKGDDGRDLVIARDYISQGMRARAAHL
                     ATLELGPRTDREIRRDLEAQIDADRWTSLDRVLAREAAQNDGVIDLRPDATAKANKLF
                     RSAMVGRMQKLERLGLAERLGPARWQLSENAEPLLRTLGQRGDIIKRIHRGFAEQRIE
                     RSVVDFALDSESTAQPVIGRLVARGLDDELKGTAYAVIDGLDGRAHHIRLANLDGATD
                     AAPGSIVELRRFDDAAGRQRVALAVRSDLPIEAQVHASGATWLDRQLVAREPASLSSG
                     GFGREVRDAMAARIEHLIGDGLAKRQGQRVTFVRNLLDTLRRQEIEAEAERIIGATGA
                     LFHPTTEGESIAGTYRQRLDLASGRFAMIDDGLGFTLVPWSPSLEQQLGRHVSGIARI
                     GRIEWSFGRARGPSL"
     gene            2293..2694
                     /locus_tag="VM910_23185"
     CDS             2293..2694
                     /locus_tag="VM910_23185"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI65458.1"
                     /translation="MRLLLRFILAGFWIAHWWYKVGYRGMPATQAFFLQQGLPVWLAW
                     FDVSFELVVAVCLVLGIFVPLLCLISLPILFASMWVYRRNGFYFSSGGIELPLLWACA
                     QIAQAMLGPGAFRVSVPLWLPQLPVVFGAHF"
     gene            2770..3645
                     /locus_tag="VM910_23190"
     CDS             2770..3645
                     /locus_tag="VM910_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI65459.1"
                     /translation="MINTRELNWSWQGQDIVLGLDEAGRGPVVLLLPALSSISTRGEM
                     HPLMERLASRFHTLTIDWPGFGTRSRPEVTWTPNALSSFLDHLLHDIVSGPHGVVAAG
                     HAATYVLHHAAHRPGTIARAALIAPTWRGPLPTMGGGQRPLFQAIRRAVETPYVGPLL
                     YRLNVNPFVVRKMVAGHVYSDANWLTGERLAQKCRVIDAAGARFASAAFVTGALDPVA
                     SRAEFLALAARAAIPITVIYGAETPARSRAEIEALAALPGIKASLLPRGKLSVHEEFP
                     DDVARAVEPFLSQRE"
     gene            3809..5812
                     /locus_tag="VM910_23195"
     CDS             3809..5812
                     /locus_tag="VM910_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HVI65460.1"
                     /translation="MTATKILWGQIVVVFAIVLAAMWLATQWTAWRLGFQPQLGLPWF
                     EVAGVPVYLPVAFFWWWYHYDAYAPRIFMEGAGFAASGGFASIGLAIGMSVWRAREAK
                     TITTYGSARWATKKEIRGAGLLHPDGVILGRFGRDYLRHDGPEHVLCFAPTRSGKGVG
                     LVIPTLLTWSGSAIVHDIKGENWQLTAAWRARFGRVLLFDPTNPLSAAYNPLLEVRRG
                     DTEVRDVQNVADILVDPEGALERRNHWEKTSHSLLVGAILHVLYAEPDKTLAGVANFL
                     SDPKRPIETTLRAMMTTRHLGDRPHPVIASAARELLNKSENERSGVLSTAMSFLGLYR
                     DPVVAKVTSRCDWRINDLADESRPVTLYLVVPPSDISRTKPLVRLVLNQIGRRLTEDL
                     KAPGHRILLMLDEFPALGRLDFFESVLAFMAGYGLKAFLIAQSLNQIEKAYGPNHSIL
                     DNCHVRVAFATNDERTARRVSDALGIATELRAMKNYAGHRLSPWLGHLMVSRQETARP
                     LLTAGEIMQLPPNDELILVSGIPPIRTRKARYYEDIRLKNRVLQPPAPTTACAGNSGD
                     RLAPHADDWTAFLLPQPGAAAAGESVAGPVADDDPANGGIRREPALPEHEAIVPEQPR
                     PDLEFAVLEDEPDDDAVRAKALNRSFATVARQAALDPGDGLGL"
BASE COUNT         1037 a         1843 c         1765 g         1173 t
ORIGIN      
        1 ccgaccgcgc gatggcaaga taaaagccgc aaggtgcggc gcggtcgggt ggcggaagag
       61 ttgagatgca tcaaggacct caggcgaggc gcgcgtgggc ctcacagcct cctgatctgc
      121 acaactcatt gcgacttcgc aacaacgcgc acctcgcgtg cgccggcgtc atgacccgcg
      181 acgatgatct ctacgtccgg ctcggccgcg tccgggatgg gggcagaggc agcggccggc
      241 gcgtcaagcc gttcattgcc caggcgctga ttgcgacgga gaaggccgga ggccttcacc
      301 gccgatccgg acgcaactcc cgcaggactg cgttcggtcg agccggtgcg gcgagtgttg
      361 cagcaagcca attaatttcg gaccgggcgc ggcacgtcac cgtcaaggcg cgcgtcgttc
      421 ggcacagggc gaggggcgcg ccgctgcgta cccatttggc ctatctgcgg cgggatggcg
      481 tgaccaggga cggcgtcgcg ggtcgcatgt tcaacgccga gcgcgatgac gccgatcatc
      541 gcgcgttcgc ggaacgctgc gacggcgatc ggcatcactt ccggttcatc gtgtcgcccg
      601 aagacgcaga acagctgtcc gatttgaagt cattcacgcg ggagctgatg aggcaggctg
      661 aacgtgatct cggcacgagg ctcgattgga tcggcgtcga tcactggaac actaacaatc
      721 cccatgtcca tgtcattgtc cgtggcaaag gcgacgatgg ccgcgacctg gtgatcgctc
      781 gggactacat cagccaggga atgcgggctc gggcagccca cctcgccaca ctcgaactcg
      841 ggccgcgaac cgaccgcgag atccgtcgcg atctcgaggc gcaaatcgac gccgatcgtt
      901 ggaccagtct tgatcgggtg ctcgcccgcg aggcggcgca aaacgacggc gtcatcgacc
      961 ttcgacccga cgcgaccgcc aaggcgaaca agctctttcg atctgcaatg gttggccgca
     1021 tgcaaaagtt ggagcgcctc ggccttgccg agcggcttgg tcccgcacga tggcaattgt
     1081 cggagaatgc ggagccgctg ttgcgcacac tgggtcagcg cggcgacatc atcaagcgta
     1141 tccatcgcgg atttgccgag cagcgaatcg aacggagcgt cgtggacttc gcgctcgata
     1201 gtgagagcac agcgcagccg gtcatcggtc ggcttgtcgc ccgcggtctt gacgacgagc
     1261 tcaaggggac ggcgtacgct gtgatcgacg gcctcgacgg tcgcgctcat catatccgct
     1321 tggccaattt ggatggagca actgatgcgg cgccgggtag catcgtcgag ctccgccgct
     1381 tcgacgacgc tgccgggcga caacgcgtcg cgctcgccgt gcggtccgac ttgccgatcg
     1441 aggcgcaggt tcacgcgagc ggcgcaactt ggctcgatcg ccagctcgtc gctcgcgagc
     1501 cggcatcctt gagttccggc ggcttcggtc gcgaggtccg cgacgcgatg gccgctcgca
     1561 tagagcacct tatcggggat gggctcgcca aacgacaggg ccagcgcgtg accttcgtgc
     1621 gcaacctcct cgacaccttg cggcgccaag aaatcgaagc agaagcggag cgcatcatcg
     1681 gagccactgg ggcacttttc catcccacaa cggaaggtga gtcgatcgcg ggcacctatc
     1741 ggcagcgcct ggacctggcg tccggccgct tcgccatgat cgacgatggt ctgggattca
     1801 ctttggtgcc gtggagcccc tcgctcgaac aacagctcgg ccgccacgtt tcgggaattg
     1861 cccggatcgg cagaattgaa tggagcttcg gaagggcacg tggtccgagc ctttagtcgc
     1921 tgcaccttca cgcgaaacat tgagccgggc gataccgaga cggggtggga atgcttctgc
     1981 ggttcagacg cgcttcccac aacgggtgtt gtgggacctt tggcctaaga atcttcgcct
     2041 cagatgtcct gccgccttaa aacgaccgtc ctttgtcttg ccacccatac ccgcgcctac
     2101 gacgatctca cttttcatct ttcatcgatc gcgttcgcat tgcattgtcc gagctctctc
     2161 gggggtagct cgtagtttgc cgggcttggc acgctgccga aaaatgtccg cggtcaatct
     2221 gccggcatca aatcaatcaa agcctctgcg acggatcaat ggtgctgcaa gattttgatt
     2281 tgagtggcct tggtgcggct tttgttacgc ttcatcctag ctggcttctg gatcgctcac
     2341 tggtggtaca aggtcggata tcgcggaatg ccggcaacgc aagcgttctt cttgcaacag
     2401 ggcctgcctg tttggctggc atggttcgat gtcagcttcg agctggttgt ggcggtgtgt
     2461 ctcgttctcg gcatctttgt gccgctccta tgcttgatca gcctgcccat ccttttcgcc
     2521 tcaatgtggg tctatcgcag gaatggattt tatttctcga gcggaggcat cgaacttccg
     2581 cttctgtggg cgtgcgcgca aattgcccag gctatgctcg gtcctggtgc tttccgcgtc
     2641 tcagttccgc tttggctgcc gcagctccca gtcgtgttcg gcgctcattt ttgactgcag
     2701 ccgaaaaggc cgcgggctaa atattcgaaa gcgctatgat cggaaacgcc acgccattag
     2761 cctgtggtca tgatcaatac ccgcgaacta aactggtcgt ggcaaggaca ggacattgtg
     2821 ctcggtctcg acgaggcggg gaggggacct gttgttctcc tgcttcctgc cttgagctcg
     2881 atttccacgc gcggggaaat gcatccgctc atggaacggc tggcctcgcg gttccataca
     2941 ctcaccatcg attggccagg attcggcaca cgttcccgcc ccgaagtgac atggactccc
     3001 aacgcccttt cgtcgttcct ggatcaccta ttgcatgaca tcgtttctgg accgcacggt
     3061 gttgtggccg cggggcacgc tgcgacctac gttctccatc acgccgcaca tcgccctggg
     3121 acgatcgcgc gagcagctct gatcgctccg acatggcgcg gaccgttgcc caccatgggc
     3181 ggcggtcagc ggccgctctt tcaggcaata cgacgcgccg tcgaaacgcc atacgttgga
     3241 ccgcttctct accggcttaa cgtcaatcca ttcgttgtcc gcaaaatggt agccggccac
     3301 gtttatagcg atgccaactg gttgactggt gagcgtctgg cgcaaaagtg tcgggtcatc
     3361 gatgccgcag gagcccgctt tgcctcggct gcctttgtga ccggcgcgtt ggatcctgtt
     3421 gcgagccgag cagaattttt ggcgctggcc gcgcgcgccg ccatcccaat cacggtgatc
     3481 tatggggcag aaacgcccgc acgctcccga gcggaaatcg aggcactcgc tgcgcttccg
     3541 ggtatcaagg cctcgctact gccgcggggc aagctatcgg tccacgaaga atttcccgac
     3601 gacgtggcaa gagcggtaga gccgttcctt tcgcagcgag aataatttcg cagcaatatt
     3661 tcagggcgat gtccttctaa ctgatttctc ctttcaccag tttctgcgcg ctgcactatc
     3721 caaaactacg atgcctacga ttccctggtt gcgcagattc gagtcgtggc ctttttaatc
     3781 accgctgctg agttcagcgg agggccgagt gaccgccacc aaaatcctct ggggccagat
     3841 cgtcgtcgtc tttgcgattg tgctcgcggc gatgtggctc gcaacgcaat ggactgcctg
     3901 gcgcctcggc tttcagccgc agctcggtct tccttggttc gaggtcgcag gtgtcccggt
     3961 ctatctccca gtcgccttct tttggtggtg gtaccactac gacgcatatg cgccacggat
     4021 tttcatggag ggcgcaggct tcgcggcttc cggcggtttc gcgtcgattg gccttgcgat
     4081 cggcatgtcg gtgtggcggg cgcgcgaggc caagaccatc acgacgtacg gctcagcgcg
     4141 ttgggcaacg aagaaagaga tccgcggtgc cggacttctc catccagacg gtgtcattct
     4201 tggccgtttc ggtcgcgact atctccggca cgacgggcca gaacacgtcc tgtgcttcgc
     4261 gccgacccga tccgggaagg gcgtcgggct cgttatcccg acgctcctga cgtggtcggg
     4321 ttcggccatc gtgcacgaca tcaagggcga aaactggcag ctcaccgccg cctggcgggc
     4381 gcgcttcggc cgcgtgctgc tgttcgatcc gactaatccg ttatcggctg cctacaatcc
     4441 attgctcgag gtgcgtcgcg gcgacaccga ggtgcgcgac gtccagaatg ttgccgacat
     4501 cctggtcgat ccagaaggcg cgctggagcg ccgcaaccac tgggagaaga ccagccactc
     4561 gctcctggtc ggcgcgatcc tgcatgtcct ttacgccgaa cccgacaaga cccttgcggg
     4621 cgtcgccaat ttcctgtccg accccaaacg gccgatcgag actacactgc gcgcgatgat
     4681 gacgacgcgg catcttggcg atcggccgca tccggttatt gcgtcggccg cacgagaact
     4741 tctcaacaag tccgaaaacg aacgttccgg ggtgctttcg acggcgatga gctttctcgg
     4801 gctttaccgc gaccctgtcg ttgccaaagt cacatcgcgc tgcgattggc ggatcaatga
     4861 cttggcggac gagagccgtc cggtcacgct ctatctcgtg gtgccgccct ccgacatcag
     4921 ccgcaccaag ccgctcgtcc gcctggttct caaccagatc ggccggcgtc tgaccgaaga
     4981 cctcaaggcc ccaggccacc gcatcctact catgctcgat gagtttcccg cgctcggacg
     5041 cctcgacttc ttcgaatccg tcctcgcctt catggcgggc tacggtctca aagccttcct
     5101 gatcgcgcaa tcgttgaacc agatcgaaaa ggcttatggc ccgaaccatt cgatcctcga
     5161 taactgccac gttcgcgtcg ccttcgccac caacgatgaa cggacggcgc gccgggtgtc
     5221 cgacgctctc ggcatagcga ccgagctgcg ggccatgaag aactatgccg ggcaccggct
     5281 ctcgccctgg ctcggccatc ttatggtctc gcgacaagag acagcgcgac cacttctgac
     5341 ggccggcgag atcatgcaac tgccgccgaa tgatgaactc attctggtat ccggcatccc
     5401 gccaatccgc acgcgcaaag cccgctacta cgaagatatc aggcttaaga atcgtgtctt
     5461 gcagcctccc gcgccgacca ccgcctgcgc gggcaactcc ggcgatcgac tcgctccaca
     5521 cgccgacgac tggacagcat tcttgcttcc acaaccaggt gcggcggctg caggcgagtc
     5581 ggtcgccggt cctgtagctg acgacgatcc cgccaacggc ggcatccgtc gcgagccagc
     5641 tctcccggag cacgaagcca ttgtccccga gcagccaaga ccggaccttg agttcgcggt
     5701 gctcgaagac gaaccggacg atgatgcggt ccgtgccaag gcactgaatc ggagcttcgc
     5761 gacggtggcg cgccaagcgg cgctcgatcc tggcgatggt cttggactgt aaggcgcg
//
LOCUS       DATLJT010001010         4196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1216877,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001010 DATLJT010000000
VERSION     DATLJT010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216877"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VM910_23200"
     CDS             <1..137
                     /locus_tag="VM910_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HVI65461.1"
                     /translation="FASGWIEGDQGYLGRPDDIILNKDGSILVSDDWAGAIYRISYKK
                     "
     gene            323..904
                     /locus_tag="VM910_23205"
     CDS             323..904
                     /locus_tag="VM910_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI65462.1"
                     /translation="MRIAWVGLAILCTGFGAAAGAADADAGKAKAEICAGCHGENGIS
                     QTENIPSLAAQPDQFVQWQLVYFRAGSRKNEQMQPIVAEISNEDIRNLGAYFASLAPP
                     KTPEADDNPNLSKKGAEAAVGRRCASCHTDNFAGTKGVARLAGQREDYLLKALHDYKS
                     GARVGGGQAVMADVAYPLSEEEIEALAHYLAHL"
     gene            complement(933..1850)
                     /gene="panE"
                     /locus_tag="VM910_23210"
     CDS             complement(933..1850)
                     /gene="panE"
                     /locus_tag="VM910_23210"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HVI65463.1"
                     /translation="MRILVVGAGAIGGYFGGRLLQAGRDITFLVRPRRASELSSAGLV
                     IKSPNGDVTLKNPPTVQADAIKDKFDVVLLSCKAYDLEDAVKSFAPAVGPQTSIIPLL
                     NGMLHLDVLDKRFGRERVLGGLCAIAVTLNEKREVVHLQPMQSLGFGERDGKMSDRVR
                     AIGEVFSAIEGASASGHVMQDMWEKWVFLASIAAATCLMRAPVGMILAAPGGKDFMLG
                     ILDECKAVATAEGFPPRAPALQRAQTMLTTEGSPLTASMFRDIKANMPVEADHVVGDL
                     IARGDAAKVPVPRLRTAYTHLKAYEKQRG"
     gene            complement(1959..2696)
                     /locus_tag="VM910_23215"
     CDS             complement(1959..2696)
                     /locus_tag="VM910_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65464.1"
                     /translation="MQKRNASVAVIGAGDYIGAEIAKKFASEGFSVFAGRRNGEKLAP
                     LVKEVEAAGGTIFGRSLDARKEEEVAAFLQDADKHAPLEICIFNVGANVNFPILETTE
                     RVFRKVWEMACYAGFLAGREAARLMLQRGRGNIFFTGATASLRGGTGFAAFASAKFGL
                     RAVAQAMARELGPKDIHVAHLIIDSGVDTAWVRERREQLWGKDALDNPDLLMPPASVA
                     GAYWQLYQQPKSAWTFEMEIRPFGEKW"
     gene            complement(2718..3323)
                     /locus_tag="VM910_23220"
     CDS             complement(2718..3323)
                     /locus_tag="VM910_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HVI65465.1"
                     /translation="MGQKVEFLFDFGSPNAYLSHEAIPAIEKRTGASFEYVPILLGGI
                     FKATNNRSPVETLAGVKNKPEFMQLETERFITRFNVQPYVWNPYFPINTLNLMRGAVA
                     AQSEGVFKKYVEAAFHHMWREPKKMDDPQVAVKALTESGLDGARLLTRAQDADVKAKL
                     IENTQKAVERGAFGSPTFFVGNEIFFGKEQLREVEELVTGK"
     gene            complement(3390..3959)
                     /locus_tag="VM910_23225"
     CDS             complement(3390..3959)
                     /locus_tag="VM910_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HVI65466.1"
                     /translation="MTETFTAFEGERRLAFGPLAEVALAVKRVEGRAFAPIAVFSDST
                     GRAVDLDLRGSDEDVVARLAETPAPPAETPRGRGRPRLGVVAREVTLLPRHWEWLNAQ
                     PGGASVALRKLVDEARRANGDRQRSARDAAYHFMSAMAGNLPNFEEASRALFADDRTR
                     FVSQIAGWPADIRDHVVKLAYRDRADQFP"
     gene            4118..>4196
                     /locus_tag="VM910_23230"
     CDS             4118..>4196
                     /locus_tag="VM910_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI65467.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGVAY"
BASE COUNT          758 a         1359 c         1274 g          805 t
ORIGIN      
        1 tatttgcctc cggttggatc gagggcgacc agggttatct cggccgtccc gacgacatca
       61 tcctgaacaa ggacggttcg atcctggtgt cggatgattg ggccggtgcg atctaccgca
      121 tcagctacaa gaagtagcga aacgacacga gaggctgcga tcgccgcgag gcggccgcag
      181 ccttttgctt tgggctcgtc agacaaccac tgtcgttccg gatcgcgagc gggcgagcct
      241 ggaacccgta gccacgatgg tgattatgga ttccgggctc acgcggacac gcgcccgccg
      301 aatgacgaac ggagatgacg ggatgcggat tgcctgggtc ggattagcga tcttgtgtac
      361 gggcttcggc gcggcggctg gtgctgccga tgcggatgca ggaaaagcca aggccgaaat
      421 ctgcgccggc tgtcacggcg agaacggcat ctcgcagacc gagaatattc cctcgctggc
      481 cgctcagccc gatcaattcg ttcagtggca gctcgtctat ttccgcgcgg gctcgcgcaa
      541 gaacgaacag atgcagccga tcgtcgcaga gatcagcaat gaggatatcc gcaatctcgg
      601 ggcttatttt gcgtcgctgg cgccgccgaa aacgcccgag gccgacgaca atccgaatct
      661 atcgaagaaa ggcgcggagg ctgccgtcgg gcgccgctgc gcttcgtgtc acaccgacaa
      721 tttcgccggc accaagggtg tcgcccgcct cgccggccag cgcgaggatt atctgttgaa
      781 ggcgttgcac gactacaaat ccggcgcgcg tgtcggcggc ggccaggctg tcatggccga
      841 cgtcgcctac cccctgagcg aagaggaaat cgaagcgctc gcgcattatc tcgcgcacct
      901 ctgacatccg ccgagcgccc cgtgagagcg ccctaccctc gctgcttctc gtaggccttc
      961 agatgcgtgt aggccgtgcg cagccgcggc accggcactt tcgcagcatc gccgcgcgcg
     1021 atcaggtcgc cgaccacgtg gtcggcttcg accggcatgt tcgccttgat gtcgcggaac
     1081 atcgaggcgg tgagcggcga accttccgtg gtgagcatgg tctgcgcgcg ctggagtgcg
     1141 ggcgcgcgtg gcggaaaccc ttccgcggtc gccaccgcct tgcattcgtc gaggatcccc
     1201 aacataaaat ccttgccgcc gggtgccgcc agaatcatgc cgacaggcgc gcgcatcagg
     1261 caggtcgcgg ccgcgatcga cgccagaaac acccattttt cccacatgtc ctgcatcaca
     1321 tgcccgctcg cgctcgcgcc ttcgatcgcg gagaacacct cgccgatcgc gcgcacgcga
     1381 tccgacatct tgccgtcgcg ctcgccaaag ccgagcgact gcatcggctg aagatgcacc
     1441 acctcgcgct tttcgttgag cgtcactgcg atcgcgcaga gcccgccgag cacgcgctcc
     1501 cgcccgaacc gcttgtcgag cacgtcgaga tgcaacatgc cgttgagcag cgggatgatc
     1561 gaggtctgtg gcccgaccgc cggcgcaaag gatttgaccg catcctcgag gtcataggcc
     1621 ttgcagctca acagcacgac gtcgaacttg tccttgatcg cgtcggcctg caccgtcggc
     1681 ggatttttca gcgtgacgtc gccattcgga cttttgatca caagaccggc ggaagaaagt
     1741 tcgcttgcgc gcctggggcg caccagaaac gtgatgtcgc ggccggcctg caacagcctg
     1801 ccgccgaaat agccgccgat cgcccctgcc ccgaccacga gaatacgcat cgtttaaatc
     1861 ctttttgtct ggtctatcgg cgaagcggcg aattgtgcat agcgattggc gaatacgggt
     1921 ccttccctat tcgctgcctg ccactcgcaa ttcggctttc accatttttc tccgaagggg
     1981 cggatttcca tctcgaaggt ccaggcgctc ttcggctgct ggtagagctg ccaataagcg
     2041 cccgcgactg aggccggcgg catcaggaga tcggggttat cgagtgcgtc cttgccccaa
     2101 agctgttctc ggcgttcccg tacccaggcg gtatcgacgc cggaatcgat gatgagatgt
     2161 gcgacgtgga tgtccttcgg acccaactcg cgcgccatcg cttgcgcgac cgcgcgcagg
     2221 ccgaatttgg cgctggcaaa ggctgcaaag ccggtgccgc cgcgcaggct cgcagtcgca
     2281 ccggtgaaga agatgttccc cctgccccgc tgcagcatca gccttgctgc ttcgcgtcct
     2341 gccagaaagc ccgcgtagca ggccatctcc cagaccttgc ggaacacgcg ctcggtggtc
     2401 tcgagaatcg ggaaattgac attggcgccg acattgaaga tgcagatttc gagcggcgca
     2461 tgcttgtccg catcctgcag gaaggccgcg acctcctctt ctttccgcgc atcgagcgaa
     2521 cggccgaaga tcgtgccgcc cgcagcttcg acctccttga ccagcggcgc gagcttttcg
     2581 ccgttgcgcc gtccggcgaa tacggaaaag ccttcggaag cgaatttctt cgcgatctcg
     2641 gcgccgatgt aatcgccggc tccgatcacg gcgacgctgg cgttgcgctt ttgcatgagg
     2701 ctctcctcaa tgacgggcta ttttcccgtc accagctcct cgacttcgcg aagctgctcc
     2761 ttgccgaaga atatctcgtt gccgacgaag aaggtcggcg agccgaaggc gccgcgctcc
     2821 acggccttct gcgtgttctc gatcagcttt gctttcacgt ccgcgtcctg cgcgcgggtc
     2881 aagagccttg cgccgtcgag cccggattcg gtcagggctt tcaccgccac ttgcggatcg
     2941 tccattttct tcggctcccg ccacatgtga tgaaacgccg cctcgacata ttttttgaac
     3001 acgccctcgg attgcgcggc gaccgccccg cgcatcaggt tcaaggtgtt gatggggaaa
     3061 tacgggttcc agacataggg ctgcacgttg aaacgcgtga tgaatcgctc cgtctcgagc
     3121 tgcatgaatt cgggcttgtt cttcacgccc gcgagcgtct cgaccggcga tcggttgttg
     3181 gtggccttga aaataccgcc gagcaggatc ggcacatact cgaacgaggc gccggtgcgc
     3241 ttttcgatcg ccgggattgc ctcatggctc aaatacgcgt tgggactgcc gaaatcgaac
     3301 aggaattcca ctttttgacc cacggcaatc tccctgtttg caacgttctt atatgatcgc
     3361 tgtcattcaa ccagcccgaa agccgtcgtt caagggaatt ggtcggcccg atcgcgataa
     3421 gcgagcttga cgacgtggtc gcgaatatcg gccggccacc ccgcgatctg actgacgaac
     3481 cgcgtgcggt cgtcggcaaa cagcgcgcgc gacgcctcct cgaagtttgg caagtttccg
     3541 gccatcgccg acatgaaatg ataggcggcg tcgcgcgcgg agcgctggcg gtcgccattg
     3601 gcgcggcggg cttcgtcaac gagcttgcgc aaggcaaccg acgcaccgcc aggctgcgcg
     3661 ttgagccact cccaatgccg cggcagcagc gtgacctcgc gcgccaccac gccaagtcgc
     3721 ggcctcccgc ggccgcgcgg cgtctcggcc gggggcgcag gcgtttcggc aagccgcgcg
     3781 accacgtcct cgtcgctgcc gcgaagatcg agatcgacag cccggccggt cgaatcgctg
     3841 aacacggcga tcggcgcgaa cgcccgcccc tcgacgcgct tgaccgcgag tgcaacctcg
     3901 gcaaggggac cgaaggcgag gcgccgctcg ccttcaaagg ccgtaaatgt ctcggtcatt
     3961 tgaggttttc cgctgatgcc agttccctat taatacccgg gtgtaattta cctgtcaata
     4021 ttcatccggg taaaaaatgc cggccggccg attgccgcat cgccaatccg ctgacggcct
     4081 gatacgggat cggccgtctg cgtgccggag aacatcgatg ctgacggttc accatctcaa
     4141 caattcgcgc tcgcagcggg tgctctggct tctggaggaa ctcggcgtcg cctacg
//
LOCUS       DATLJT010001011         6619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1216898,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001011 DATLJT010000000
VERSION     DATLJT010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216898"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VM910_23235"
     CDS             complement(<1..687)
                     /locus_tag="VM910_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVI65468.1"
                     /translation="MRFGLWRKGEGTAQAEAAAAQPAQAPDSPAIAGDIDLRALGDAL
                     ARKRDWIILPSVLAAVLSIAAVNFITPRYKSEARILIDGRENVFLRPNADRAEERAAL
                     DAEAVTSQVQLVLSRDLAREIIKNNKLAERPEFDPVLQGISPLKSLLALSGIGRDPFS
                     MTPEERVLDAYFDRLTAYAVDKSRVIVVEFQSWDPDLAARVANSIADGYLVLQQNARQ
                     QQAKSASQWLA"
     gene            1023..2162
                     /locus_tag="VM910_23240"
     CDS             1023..2162
                     /locus_tag="VM910_23240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVI65469.1"
                     /translation="MMPAPDRPLRILHAVRAPVGGIFRHILDLANGQADRGHEVGILA
                     DSFTGGERAAQALAEIAPRLKLGVHRMGMHREPRASDVIVWTRFMRLVRRLEPDVLHG
                     HGAKAGAILRLRRRSDSAIRVYTPHGGSLHFPLSTLKGAVYARLERALMNNTELFLFE
                     SAFARNTYQRTIGMPKGLVRCVFNGVTADEFEPVARASDAADVVYVGEFRHIKGADLL
                     IDAVARLRADGKPVTLTLAGDGEEMATLKAQVQRLGLTRSARFIGHVKARYGFSKGKL
                     LVVPSRGDSMPYVVIEAGAAGIPMVAANVGGIPEIFNFHTDALFAPNNVGAMADAIKT
                     ALEGPDAAEARAKSLRERIFLHFSQKAMVEGVLAGYRDAFEALRP"
     gene            2335..3855
                     /locus_tag="VM910_23245"
     CDS             2335..3855
                     /locus_tag="VM910_23245"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HVI65470.1"
                     /translation="MLDAAASAAATAGQPPVERRRRLSPAALALTSQKVHPAYSPIVI
                     AGAARLIDFFVLNLIGVALYFGYVVPQSGFHWEYIAAIVAMTAAAVICFQAADIYQVQ
                     VFRGQLRQLTRMISSWAFVFLLFSGVAFLAKLSGEISRLWLSAFFFIGLGALIAERLL
                     LRALVRRWARQGRLDRRTVIVGSDANGEQLIEALKNQYDSDIDVLGVFDDRNDARALE
                     TCAGSPKLGKVDDIVEFARRTRVDLVLFALPISAETRILEMLKKLWVLPVDIRLSAHT
                     NKLRFRPRSYSYVGKVPTLDMFEAPITDWDLVMKWLFDHVTGGLILLAALPVMALVAL
                     AIRLDSPGPVLFRQKRFGFNNERIDVFKFRSLYHHQADPTASKVVTKNDPRVTRVGRF
                     IRKTSLDELPQLFNVVLEGNLSLVGPRPHAVQGKLKTRLFDEAVDGYFARHRVKPGIT
                     GWAQINGWRGEIDNEEKIQKRVEFDLYYIENWSVLFDLFILLKTPIALMTRNENAY"
     gene            3912..5186
                     /locus_tag="VM910_23250"
     CDS             3912..5186
                     /locus_tag="VM910_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HVI65471.1"
                     /translation="MAYALTAEGIPSSATAAPGVLALQRALVWLAAASIAIVFIEPSP
                     YELVTLTAIVLFFATGLRLRLVFMPLLAMLVVINLGYSISAIPLLGQSEVASWIATSW
                     YMAVTVIFLAMVVSEDTQARLDMLRRGLTLGAMIASITAIAGYFNLVPGGHDLLTLYD
                     RARGTFKDPNVLGAFLILPALFLLQNVVTARFGKALRSTIALAVIGLAILLAFSRAAW
                     GGLILTSAFMLAMMVLTGRSKAERSRIVIVTLVAALAGVMLIAALLSFDSIADMFRQR
                     ASFDQSYDEGRFGRFGRHILGADMALGLPFGIGPLQFHRYFPEDTHNSYLNAFMSGGW
                     ISGVTYPALVFVTVILGFRHAFVRVPWQRAYLAIFAAFLGTVGEGFVIDTDHWRHFWM
                     MLGAMWGMFAAAQPYNMAARKPLSAPAEASAP"
     gene            complement(5155..5652)
                     /locus_tag="VM910_23255"
     CDS             complement(5155..5652)
                     /locus_tag="VM910_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI65472.1"
                     /translation="MQELEEAMRRSSAQGVLFGQTVANAIGISGSDLDCLDFVNLEGR
                     VTAGRLAEVTGLTSGAITGVVDRLEKAGLVRRERDEVDRRKVFIVVVSENAAKIGRFY
                     VPMQQAMHKLWSTYSDAELQLLLRFAKEGYKAALQATEVLNTLTNDGVKKRAALKEPK
                     PRRAR"
     gene            5777..6016
                     /locus_tag="VM910_23260"
     CDS             5777..6016
                     /locus_tag="VM910_23260"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI65473.1"
                     /translation="MTHRPRKALIIGAGIAGPVAAILLKRAGIDVAVYEAWPYSTGIG
                     GGLQIAPNGMRVLAEIGIADELIRRGAIAESFDFY"
     gene            6237..>6619
                     /locus_tag="VM910_23265"
     CDS             6237..>6619
                     /locus_tag="VM910_23265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HVI65474.1"
                     /translation="MIGADGVHSTVRQHVVPGGPKPFDTDLVGFGGFVPRSLLEDAGI
                     GPRVRATFGQSGFFGYGFSSPDPGQGAMWWSTQPSNGIDAATFRAMSVRKLKQHLHDF
                     HAGWHDPIPRIIDAAETIVVTDTLDV"
BASE COUNT         1122 a         2163 c         2022 g         1312 t
ORIGIN      
        1 cgccagccac tggctcgcgg atttcgcctg ctgctgccgc gcattctgct gcagcaccag
       61 atagccatcg gcgatcgaat tggcgacgcg cgcggcaaga tcgggatccc acgactggaa
      121 ctcgacgacg atgacgcgcg atttgtcgac ggcataggcc gtcaggcggt caaaataggc
      181 atccagcacc cgttcttccg gcgtcatcga aaacgggtcg cggccgatgc ccgacaaagc
      241 cagcagggat ttcagcggcg agatgccctg caacaccgga tcgaattccg gccgctccgc
      301 aagcttgttg ttcttgatga tctctcgcgc gagatcccgc gacaggacga gctgcacctg
      361 gctggtgacc gcttcggcat cgagtgctgc acgctcctcg gcgcggtcgg cgttcggccg
      421 cagaaacacg ttctcgcgcc catcgatcag gattcgcgcc tcggatttgt aacggggagt
      481 gatgaaatta accgcagcaa tcgagagcac ggcggcaagg acgctcggaa gaatgatcca
      541 atcgcgcttc cgcgcgagcg cgtcgccaag cgcgcgcaga tcgatgtcgc cggcgattgc
      601 tggggaatca ggagcttgcg caggctgggc tgcggccgct tcggcctgcg ccgtcccctc
      661 acccttacgc cacaagccaa aacgcatcgc ttaactcccg ccgacgccac gtctttcaac
      721 aaacccggtg ccccgaattg cagctccttc gttatggacc ttcaaatcgg cggcactggc
      781 gcgactacac tctattaagg ttgccgctga gttaattcag gccgatttga ggcgtggatt
      841 gggggatgcg gaataccgcc agtcatgttt tgttaaccat gaaagtcctt aatcggaatc
      901 aggatttcgc ttgagaggtg tcggatgccg ggcccgcgcg ccgtcgacct gttgtcgctg
      961 acgcatacgg ccgtcacatc cgacgcggcc gatgccgcgc gccgcgggga atgccagttc
     1021 tgatcatgcc cgcgccagat cgtccgcttc gtatcctgca tgcggtgcgt gcgccggttg
     1081 gcggcatctt tcgccatatt ctcgacctcg ccaacggaca ggccgatcgc ggccacgagg
     1141 tcggcatcct cgccgacagc ttcaccggcg gcgaacgtgc cgcgcaggcg ctggccgaaa
     1201 tcgcgccgcg cctgaagctc ggcgtgcatc gcatgggcat gcaccgcgaa ccccgcgcgt
     1261 cggacgtcat tgtctggacg cgcttcatgc gcctggtccg tcggctcgag ccggacgtcc
     1321 tgcatggcca tggtgccaag gccggcgcga tcttgcgcct gaggcggcgc tcggatagcg
     1381 ccatccgcgt ctacacccca cacggcggct cgctgcattt tccacttagc actctcaagg
     1441 gcgcggtcta tgcgcggctg gaacgcgcgc tgatgaacaa caccgaactg ttcctgttcg
     1501 aaagcgcctt tgcccgcaac acctatcagc gcaccatcgg catgccgaaa ggcctggtgc
     1561 gctgcgtgtt caatggcgtc actgcggacg agttcgagcc cgtggcacgc gccagcgacg
     1621 ccgccgacgt cgtctatgtc ggcgaattcc gtcacatcaa gggcgccgac ctcctgatcg
     1681 atgcggtggc gcgactgcgt gccgacggca agccggtcac gctgacgctc gccggcgacg
     1741 gcgaggaaat ggcaacactg aaggcgcagg tgcaaagact cgggcttacg cgctccgcgc
     1801 gcttcatcgg ccacgttaag gcgcgctacg gcttctccaa gggaaagctg ctggtggtgc
     1861 cttcgcgcgg cgattccatg ccctatgtcg tgatcgaggc cggagccgcg ggcattccga
     1921 tggtcgcggc caatgtcggc ggcatcccgg agatcttcaa tttccacacc gatgcgctgt
     1981 tcgcgcccaa taacgtcggt gcgatggcgg acgcgatcaa gaccgcgctg gagggaccgg
     2041 atgccgcgga ggcacgcgcc aaatcactgc gcgagcggat tttcctgcat ttctcgcaga
     2101 aggcgatggt cgaaggcgtg ctggcgggct accgcgatgc cttcgaggcg ctccgtccct
     2161 gacgcccatc gcggcgcaag acctcattaa ccctcattga ccgggctaac agattattcc
     2221 gatttgtcct ttaagccggg gtgagtgggg ggtacgcctc ggcgcgcgca acgatctgcg
     2281 cctgcagatt cggattcgga cgaaggacaa gtggaaccga tcaacgttca ctcgatgttg
     2341 gatgctgcgg cgtcggctgc cgcgaccgca ggtcagcctc cggtcgagcg gcgtcgccgc
     2401 ctgtcgccgg ccgcgcttgc cctcaccagc cagaaggtcc atccggccta ttcgccgatc
     2461 gtgatcgcgg gcgcagcccg cctcatcgac ttcttcgtgc tcaacctgat cggtgtcgca
     2521 ctctatttcg gctatgtagt cccccagtcc ggcttccact gggaatatat cgcggcgatt
     2581 gtcgcgatga ccgccgcggc cgtgatctgc ttccaggctg cggatatcta ccaggtccag
     2641 gtgtttcgcg gccagctcag gcagttgacg cggatgatct cgtcctgggc gttcgtgttc
     2701 ctgctgttca gcggcgtggc cttcctcgcc aagctcagtg gcgagatttc ccggctctgg
     2761 ctgtcggcct tcttcttcat tggccttggc gccctgattg ccgaacgtct gcttctgcgc
     2821 gcgctggtgc gccgctgggc gcgccagggc cggctcgatc gtcgcaccgt gatcgtcggt
     2881 tccgacgcca atggcgagca gctcatcgaa gcattgaaga accaatatga cagcgacatc
     2941 gacgtactcg gtgtgttcga tgatcgcaac gacgcccgcg cgctggagac ttgcgccggc
     3001 agtcccaagc tcggcaaggt cgacgacatc gtcgaattcg cgcgccgcac ccgtgtggac
     3061 ctcgtgctgt tcgcgctgcc gatctcggca gagacgcgca tcctcgagat gctgaagaaa
     3121 ttgtgggtgc tgccggtcga catccgcctt tcggcgcaca ccaacaagct gcgcttccga
     3181 ccgcgctcgt attcctatgt cggaaaggtc ccgacgctcg acatgttcga ggcgccgatc
     3241 accgactggg acctggtgat gaaatggctg ttcgaccacg tcacaggcgg cctcatcctg
     3301 ctggcggcgc tgccggtcat ggcgctggtt gcgctcgcga tcaggctcga cagtccgggc
     3361 ccggtgctgt ttcgccagaa acgcttcggc ttcaacaacg agcgcatcga cgtcttcaag
     3421 tttcgctcgc tctaccatca ccaggccgat ccgaccgcat cgaaggtcgt caccaagaac
     3481 gacccgcggg tcactcgcgt cggccgcttc atccgcaaga ccagcctcga cgagctgccg
     3541 caactcttca acgtggtgct cgagggcaat ctctccctcg tcggaccgcg gccgcacgcg
     3601 gtacagggca agctcaagac ccgcctgttc gacgaagccg tcgacggtta tttcgcccgc
     3661 caccgcgtca agcccggcat tacaggctgg gcccagatca atggttggcg cggcgagatc
     3721 gacaacgaag agaaaatcca gaagcgcgtg gagttcgatc tctactacat cgaaaactgg
     3781 tctgtgctgt tcgacctctt cattctcttg aagacgccga ttgcgctgat gaccagaaac
     3841 gaaaacgcct actgagcgtt tccgccggcc gtttcgagcg agttgcgtag cgtggagttt
     3901 gtgagcgtgt aatggcgtat gctttgacag ccgaaggcat cccttcgtcg gcgacggctg
     3961 cgcccggcgt actcgcgctg cagcgcgcgc tggtctggct ggctgcggct tccatcgcga
     4021 tcgtcttcat cgaacccagc ccctatgaac tggtgacatt gacggccatc gtcctgttct
     4081 ttgcgacggg cctgcggctg cgcctcgtct tcatgccgct gctggcgatg ctcgtcgtca
     4141 tcaatctcgg ctacagcatc agtgccattc cgctgctcgg ccaatccgag gtggcgagct
     4201 ggatcgcgac gtcctggtac atggcggtca ccgtgatctt cctcgccatg gtcgtgtccg
     4261 aggacacgca agcgcggctc gacatgctgc ggcggggtct cacgctcggc gcgatgattg
     4321 cctcaatcac cgcgatagct ggctatttca acctggtgcc gggcgggcat gacctgctca
     4381 cgctctatga tcgcgcgcgc ggcacattca aggatccgaa cgtgctcggc gcgttcctga
     4441 tcctgcccgc gctgttcttg ttgcaaaacg tcgtcaccgc acgcttcggc aaggcgctgc
     4501 gcagcacgat cgcactcgcc gtcatcgggc ttgcgatcct gctggcgttc tcgcgcgcgg
     4561 cctggggcgg gctgatcctg acctcggctt tcatgctggc gatgatggtg ttgaccggcc
     4621 gatccaaagc ggagcgctcg cgcatcgtca tcgtcacgct ggttgctgca ctcgcgggcg
     4681 tgatgctgat cgcggcgctg ttgtcctttg attccattgc cgacatgttc cgccagcgcg
     4741 cgagcttcga ccagagctat gacgagggcc gcttcggccg gttcggccgc catatcctcg
     4801 gcgccgatat ggcgcttggt ctgccgttcg gcatcgggcc gctgcagttt caccgctatt
     4861 tccccgagga cacccataac tcctacctga acgccttcat gtcgggcgga tggatctccg
     4921 gggtcaccta ccccgcgctg gtcttcgtca cggtgatcct gggctttcgc cacgcctttg
     4981 tgcgcgtgcc ctggcagcgc gcttatctcg cgatcttcgc ggcgttcctt ggcacggtcg
     5041 gcgaaggctt cgtgatcgac accgatcact ggcggcattt ctggatgatg ctgggcgcga
     5101 tgtgggggat gttcgcagcc gcgcagccct acaacatggc cgcgaggaag ccgctcagcg
     5161 cgcccgccga ggcttcggct ccttgagcgc cgcacgcttt tttacgccat cgttcgtgag
     5221 cgtgttgagc acttcggtag cttgcagcgc cgccttgtaa ccctctttgg cgaagcgaag
     5281 cagcaactgc agctccgcat ctgaataggt gctccacagc ttgtgcatcg cctgctgcat
     5341 gggcacgtag aaccgtccga tcttcgcggc gttctccgac acgaccacga tgaagacctt
     5401 gcgccggtcg acctcgtcgc gctcacgtcg caccagcccc gccttctcca gccggtcgac
     5461 gacgccggta atcgcgccgg aggtaagacc cgtgacctcg gcaagccggc ctgccgtcac
     5521 ccgtccctcg agattgacga aatccagaca atcgagatcg gaaccagaaa ttccgatcgc
     5581 attggcaacg gtctgcccga acagcacgcc ctgcgccgac gagcggcgca tcgcttcctc
     5641 gagttcctgc atcagcgcgg cgcgcgtttt ccctcttgac aataccggtc caatctctta
     5701 gtcgattaat atcttagtaa ctaagataat tagtggctga catttcttac caccacctcc
     5761 acacaaggag caagagatga cccaccgccc ccgcaaggcc ctgatcatcg gcgccggcat
     5821 cgcaggtccc gttgccgcca tcctgctaaa gcgtgccggc atcgacgtcg cggtttacga
     5881 ggcctggccc tattcgaccg ggattggcgg cggattgcag atcgcgccga acgggatgcg
     5941 tgtgctggcc gagatcggga tcgccgacga actgatccgc cgcggcgcga ttgccgaatc
     6001 cttcgacttt tattgaggac cggttcgagg ctcggttcga tcaatcgcaa catgaaggcg
     6061 cggttcggcc agccggcggt gaacatgagc cgcgccgtgc tgaacgagac gcttgtggac
     6121 aaggcatggt cgtcctgcgt cgagctgtac ttcgaaaagc gcctggcgcg aatcgaggat
     6181 ccagccggcc gatcgtcgcg actttcaccg atgggtccat cgccgaagcc gattttttga
     6241 tcggcgccga cggcgtccac tcgacggtgc ggcagcacgt cgtccccggc ggaccaaaac
     6301 cgttcgacac ggatctggtc ggctttggcg gattcgtgcc gcggtcgctc ctggaggatg
     6361 cggggatcgg accgcgcgtc cgggcaacat tcgggcagag cggattcttc ggctatggtt
     6421 tctcgagccc cgatcccggg cagggcgcca tgtggtggag cacgcagccg tcgaacggta
     6481 tcgacgccgc gaccttccgg gccatgagcg tgcggaaatt gaagcagcac ttgcacgatt
     6541 tccacgccgg ctggcacgac ccgatcccgc gcatcatcga tgcggccgag accatcgtcg
     6601 tgaccgacac gctcgacgt
//
LOCUS       DATLJT010001012         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1446988,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001012 DATLJT010000000
VERSION     DATLJT010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446988"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..903)
                     /locus_tag="VM910_23270"
     CDS             complement(31..903)
                     /locus_tag="VM910_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65475.1"
                     /translation="MSKTELIAEMREDERERCDLRAQRARVSASGDGLVRKSMGDALQ
                     RNEPVPDALKLFGDHRDEQLVSVLGIVISITRRQLRKEIAEELAERDPQLRKIAALEL
                     EVAALRGAVDVLRGAALPPPAKFPSAKVWKEDTVYHEGDVVAFGGSTYQAKRDTAQLP
                     GTGDWTCLARAGRDGESLTVKGTYNPRDTYACRDVVAYNGASFCARKSAPGTCPGPDR
                     QLLAKIGKAGPRGERGIMGLRGERGEAAPAIHAWEIDRARYVATPIMSDGSSGPALEL
                     RALFEQFLLETSDV"
     gene            complement(1157..1477)
                     /locus_tag="VM910_23275"
     CDS             complement(1157..1477)
                     /locus_tag="VM910_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65476.1"
                     /translation="MSDPTVVPFPQDGRAPPPARLSPAEADTWRAVVGSRPAGHFGPE
                     VFPVLLAYCATAMMCDHVGSRLRGEEGVDHALLESYDRMTQSLIKLAEALGLLPGAKR
                     AGPP"
     gene            complement(1474..1950)
                     /locus_tag="VM910_23280"
     CDS             complement(1474..1950)
                     /locus_tag="VM910_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65477.1"
                     /translation="MPRKSANEIATEVVVAPAPFKHERPDPPNGMPLPQAAIWRDAVS
                     SMRAGWFSRETHALLCRYCSSMAECERLEAELARMDIASPEYDRVSKRRSATATTALA
                     YARSLKITPLSNKCNTANARDAGRSLYARPWQQRGDDGDEPRKRPWEVDAREVNDS"
     gene            complement(3140..3772)
                     /locus_tag="VM910_23285"
     CDS             complement(3140..3772)
                     /locus_tag="VM910_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65478.1"
                     /translation="MTREISARLTEQVNELAPYFTNKLRPALKLANHQLEYLCPAMMA
                     TSRKGLIAELVAHVPRYTWLIVLANPRKLFTLRIYIPPEAPFTRRQYLDDCVAGAVEA
                     YGHVPTANDIVHGIDLIIEQHGGRPEAKTPQLSQDACANIVGFLFAPIPHPGRGEIIE
                     IVDKMIAENVCPLHVGLIGRGTRPNTKCGVWPIGLPFALYVEHIGQLASE"
     gene            complement(3908..4129)
                     /locus_tag="VM910_23290"
     CDS             complement(3908..4129)
                     /locus_tag="VM910_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65479.1"
                     /translation="MDIVGRWLEDRCQLDPNKKEKVSVLHADYETWAKNEIGFSMSVI
                     AFGRELASRGFERVKVDNERHIRGLCLPM"
BASE COUNT          809 a         1356 c         1310 g          867 t
ORIGIN      
        1 ctctcgaaaa cggcctcaga gatgatgatg tcagacatca ctcgtctcca ataaaaactg
       61 ctcgaagagc gcgcgcagct ccagcgccgg acctgaagag ccgtcgctca tgattggggt
      121 cgcgacgtag cgcgcgcggt cgatctccca agcgtgaatg gccggtgccg cctcgccgcg
      181 ctcgcccctc aggcccatga tgccccgctc gccgcgcggg ccggccttgc cgatcttcgc
      241 cagcagctgc cggtcggggc ccgggcaggt gccgggcgcg ctcttgcgcg cgcagaagct
      301 cgcgccgttg tacgccacga catcgcgaca tgcatacgtg tcgcgagggt tgtaagtacc
      361 tttcacggtc aggctctcgc cgtcgcgccc ggcgcgggcg aggcaggtcc agtcgcccgt
      421 cccgggcaac tgggcggtgt cgcgcttcgc ttggtacgtg ctgccgccga aggcgacgac
      481 gtcgccctca tgataaactg tgtcctcttt ccaaactttg gcgctcggaa acttggccgg
      541 cggcggcagt gccgcgccgc gcagaacgtc gaccgcaccg cgcagtgcgg cgacctcgag
      601 ctcgagcgct gcgatctttc gcaattgcgg gtcgcgctcg gcaagctcct cggcaatctc
      661 cttcctcagc tgcctccgcg tgatggaaat tactatacct aggacgctca cgagctgctc
      721 gtcgcgatgg tctccaaata acttcaaagc atctggcact ggctcgttgc gctgcagcgc
      781 gtcacccatc gacttgcgga ccaaaccgtc accagacgcg gagacccgcg cgcgctgcgc
      841 cctgagatcg caccgctcgc gctcgtcttc ccgcatctct gcgatcagct ctgtcttcga
      901 catgcttacc attcctccac cgacggcggg ccgatcccaa cgcagagggc gcggcgcgcc
      961 gcggcgcata tctccgatat atctttgttg gtcggcgatc gaaccagcgc cagccggtca
     1021 gcgacggcgc tgaaaaactt gtgccgccat cgcggcggaa cggtccgagc cctggtttga
     1081 atgatgagag tttgttccgg cgtcagcgct gccactgcag cattggccgc accgctctcg
     1141 gtatcgactc gcgccgtcat ggcggccccg ctcgcttggc cccaggcagc aagcccagag
     1201 cctcggcgag cttgatgagc gactgcgtca tccggtcgta gctctcgagc agcgcgtggt
     1261 cgaccccctc ctcgccgcgc aaccgcgacc caacgtggtc acacatcatc gcagtcgcgc
     1321 agtacgccag cagcaccgga aagacctcgg gaccgaagtg gccggccggt cgcgagccga
     1381 cgacggctcg ccaggtgtca gcctccgcag gcgacaagcg cgccggcggc ggcgctcgtc
     1441 cgtcctgagg gaaagggacg acggtcgggt cgctcatgag tcattcacct ctcgcgcatc
     1501 cacctcccac ggcctcttgc gaggctcgtc gccgtcgtcg ccgcgctgct gccacggtct
     1561 tgcgtagagg ctgcgcccag cgtcgcgggc gttggccgtg ttgcacttgt tgctgagcgg
     1621 cgtgatcttg agtgaccgcg cgtaagccaa ggcggtcgtg gccgtcgcgc tgcgacgctt
     1681 cgacactcga tcgtactccg gcgaagcaat gtccatgcgg gcgagctcgg cctcaagccg
     1741 ctcgcactcc gccatggagc tgcaatagcg gcacagcaac gcgtgggtct cgcggctgaa
     1801 ccatcccgcc ctcatggagc tgacggcgtc gcgccaaatt gcagcttgcg gcaacggcat
     1861 tccgttcgga gggtcggggc gctcgtgctt gaaaggcgca ggggcaacga ccacctccgt
     1921 cgcgatctcg ttggctgact tgcggggcat ctcaagaacc tcctagggca aaaaactgcg
     1981 ttttcggggc gcttttggtc gtggaaacga gaaaaggttg aaaagatggg gtgccgccgg
     2041 gctccgagaa ggccgcgttc gcgcgcaaaa gaaggctccc ccccggcctt ggcaagtcag
     2101 cctcgaggtt gctgcatcgg caacctatcc ccccgcgacg aacaagttgg ctaactttat
     2161 tcatgttttc gatccaaaac ggctgctgcg acttttggct tcgtcggctc cacgattggc
     2221 gcgcggaagt actccgctgc gcagtcgcgg tgccacaaac cgatgcgacc gcgggacatg
     2281 gcgatctgca ccatgtcgtt cccgctccgg ccacagagcg cgcagccgaa accgagcgga
     2341 gcggggccaa gtacctcaac gcttcggcgt ttgctcattg gaattctctt tcgagaactg
     2401 cgcccggtcg gtcccgctag ccaagagaag caagagaaga aggaccggaa accgaccggg
     2461 tcggttggga agcgccggcc atcgagcgcg aagccggcac ttttccaact ctggcgggca
     2521 acagcggtga atcatacccc atccccggcc agtgaccatg tttttgaagg gggcgaatga
     2581 ttgggggcgg aaatgggccg agggagggcg agaggcatca cagaaacagc catatggcct
     2641 cggatctgac ctctcgacta tcatccgagg ggggaggtac agatgtacag ataaagggta
     2701 ctttttccca aagtttcttt ttttcgctct cgcgtatagg actttgggaa aaagtaccct
     2761 ttatctgtac atctgtacct attgggaaaa aggggttaag gtatggcgtt gtcaacagaa
     2821 aacaccattt cgcgctaaaa acatcgcctt gcgacagggg aaaaagggcc gattggcgtc
     2881 gagtttgagt tcggcagcgg cgcgctgtcc ttcctccaat cagaagtagt gtcgcatact
     2941 gtgtcgctac tgcagagtag cggatcgtaa aggggtgtca aagtcatcgc atccggtgcg
     3001 acaacactcc gagcgatgcc gggcgcagcc gtggcgcgcc cttgatccga gattgtagcc
     3061 aagccgaacc ccggatcgga tcggccggct cgaccttgcg tcccatgaac gtgccgactg
     3121 gaaacgaagg cacgcgccat cactcgctcg ccaactgacc gatatgttca acataaagcg
     3181 cgaacggcaa cccgatcggc cacacgccgc acttcgtgtt cggcctggtg cctcgaccaa
     3241 tgagcccgac gtgcaacggg caaacgttct cggcgatcat cttatcgacg atctcgataa
     3301 tctcgccgcg tcccgggtgc ggtatcggag cgaagagaaa gccgacgatg tttgcgcacg
     3361 cgtcctgact gagctgcggg gtcttcgcct ccggccgccc gccgtgctgc tcgataataa
     3421 gatcgatgcc gtgcacaata tcgttcgcag tcggaacatg tccgtaagct tcgaccgcac
     3481 cggcaacgca atcatcaaga tattgccggc gcgtgaaagg cgcttcgggt gggatgtata
     3541 ttcgcaaagt aaaaagcttg cgcggattgg cgagcacgat cagccacgtg tagcgcggca
     3601 catgcgcgac caattccgca ataaggccct tgcggctcgt tgccatcatc gccggacata
     3661 gatattcaag ctgatgattg gcaagcttga gcgccggcct gagcttgttg gtgaaatagg
     3721 gggcgagctc attgacctgt tccgtcaatc gcgcgctgat ctcgcgagtc atgttttgct
     3781 cctgcggcta gtaaaggctt tgttgttgct gctgtggagg cttgagcttg gtgccagtga
     3841 ccccctttct cgtcgggcat gacactgcct tactggtctt catcttgctc ccatcacgcg
     3901 ccaaagctca cattggcaag cacaggcctc ggatgtgtct ctcgttgtcc actttcaccc
     3961 tctcgaagcc gcggctggcc agctcgcgac cgaaggcaat cacggacatc gagaagccga
     4021 tctcgttctt tgcccatgtc tcgtagtcgg cgtggagcac tgaaactttc tccttcttat
     4081 taggatctag ttggcagcga tcctcgagcc agcgcccgac gatgtccata tctgtatgtc
     4141 gtcagagcat ttcggacagt tggcttggca atttaagaga atgtctggct catctggttt
     4201 gaagattcag gctgtgattg cctgctgatg caggcggcgt ctttgctcga tggtttttcg
     4261 tttaatcttt tccctccgtt cgagaagggc ctggccgcgc ccgaagtaga cgtcagccgg
     4321 cgtcaggttg tccaggctct cg
//
LOCUS       DATLJT010001013         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1907115,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001013 DATLJT010000000
VERSION     DATLJT010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907115"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VM910_23295"
     CDS             <1..569
                     /locus_tag="VM910_23295"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVI65480.1"
                     /translation="ETLFQAVPGIGPALAHRVCETLRLDTIEALEAAAYDGRLEQVRG
                     FGRRRAAMVRAALAEMLARVRRGPLRRDEEPGVDLLLDVDREYREKATAGELVKIAPK
                     RFNPKGEAWLPVLHTTRGKWHFTALYSNTARAHQLGRVTDWVVIFFHKDNQAEGRRTV
                     VTEARGEAAGRRIVRGREPECLQYYASR"
     gene            complement(591..968)
                     /locus_tag="VM910_23300"
     CDS             complement(591..968)
                     /locus_tag="VM910_23300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI65481.1"
                     /translation="MVEDTKPIIYVIDDDEAVRDSLKLLLESHGMAVEDYQSSEEFAR
                     AYVPGRRACLILDLHLPGASGLDYLANQDDAATELPVIVITGRGDPGTRARAERLGVL
                     AFLDKEVSSDILLANIKQALGQA"
     gene            complement(1269..2120)
                     /locus_tag="VM910_23305"
     CDS             complement(1269..2120)
                     /locus_tag="VM910_23305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65482.1"
                     /translation="MIRTILVPLTAELSSETLLDAALLVAKRLNSHIRVLFIQPNPDT
                     AFAYLPDVILAAGVTREVIERETQEAAAVAQDRFVDWRTRSNVPEAAGARLDSCFATW
                     SEQLGDIETVVARFGRVSDLIVVQRPSSSSVQEQRCFAAAVFESGRATLVVGEKLPFA
                     ITDHVMIAWNGSLEASRAVLGAMPLLHLAGRVSIFAGPQYESEGVDAADLAESLHWHG
                     IRAHQIPGPRDENSTGAALVSAAVEQQATMIVMGAYTHSRLRQSFLGGVTRHLLAHAP
                     VPLLMSH"
     gene            complement(2236..>2499)
                     /locus_tag="VM910_23310"
     CDS             complement(2236..>2499)
                     /locus_tag="VM910_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVI65483.1"
                     /translation="IGARRLHVLLQFLAEAVFLSVSGGIAGIAMGVAFSAGISFVFGW
                     RAPVSPAAIAGGFLFAAAVGVFFGYYPARKAAHLDPIEALRYE"
BASE COUNT          498 a          781 c          783 g          437 t
ORIGIN      
        1 cggaaacctt gttccaggca gtccctggca ttgggccggc gctcgctcac cgggtctgcg
       61 aaacgcttag gctcgacaca attgaggcgc tcgaggcggc ggcgtatgac gggcggctgg
      121 agcaggtccg cggctttggc cgccgacgtg cggcgatggt gcgggccgcg cttgccgaaa
      181 tgctcgcgcg cgtccgccgc ggcccgctcc gccgggatga ggagcccggg gtggatttgt
      241 tgttggatgt cgaccgcgaa taccgggaaa aagcgacagc cggcgagctg gtcaagatcg
      301 ccccgaagcg cttcaacccg aaaggcgagg cttggctgcc ggtgctccac accacgcgcg
      361 gcaaatggca ttttactgcg ctttattcga atactgcgcg ggcccatcag ctcgggcggg
      421 tgaccgattg ggtcgtgatt tttttccaca aggacaacca agcggaaggc cggcgcaccg
      481 tggtcaccga agcccgaggc gaagcggcgg gcaggcggat cgtccgagga cgggagccgg
      541 agtgcctaca gtattacgct tcgcgttgag gcgagattta cgcaggcaag tcaagcttgc
      601 cccaaggctt gtttgatatt ggcaagcaat atatcgctgg acacctcctt atcgaggaaa
      661 gcgagcacgc ccagtcgctc ggcgcgtgct cgggttcccg gatcgccgcg cccggtgatg
      721 acgataaccg gcaattctgt tgccgcatcg tcttgattgg cgagataatc aaggccgcta
      781 gccccaggaa gatgcaggtc caggatcagg caggccctcc ggcccggcac gtaagcccgc
      841 gcgaactctt cactcgactg gtaatcctcg accgccatgc cgtgggattc gagcagcagc
      901 ttcagcgagt cgcgtaccgc ctcgtcatca tcgatcacat agatgatcgg cttcgtatcc
      961 tcgaccacca acaccctcca cacacctatc gacgacccaa aatagtgaac aacgcggcgc
     1021 ccggatttac gggatgatcc gtagtgacgg tcgggcgggc tcgggcgcga tgcgcacgcg
     1081 agcgcaacgg ccgccaaagc ccccggactt cacccaggac gcagagccct tctcgtgttt
     1141 tcgctgttct tgcggcaaat tatcaaccat cgacaactca ttctcccgcg gtttgccgtc
     1201 attgcggcag gtcctggttc actccgcgaa aatccggcat caggtggtgg gttccttcaa
     1261 ggacaccctc aatggctcat cagcagtggg actggcgcgt gagccaggag gtgtctcgtc
     1321 acgccaccga ggaagctctg tcgcaggcgg ctatgcgtat aggcgcccat cacgatcatc
     1381 gttgcttgtt gctcgaccgc ggccgacact aaggccgctc cagtagagtt ttcgtcccga
     1441 ggaccgggga tctggtgagc ccgaatgcca tgccaatgaa gcgactcggc tagatccgct
     1501 gcgtcgacgc cctcgctctc gtattgcggt ccggcaaaga tcgacacgcg accagccaga
     1561 tgcaggagcg gcatcgcgcc cagcactgcc cggcttgcct cgaggctgcc gttccaagcg
     1621 atcatgacgt ggtcggtgat ggcgaaaggc aacttctcgc cgacgacgag ggtcgcgcgg
     1681 ccgctctcga agaccgcggc ggcaaagcaa cgttgctcct ggacggagct ggacgagggc
     1741 cgttgcacca cgatcaggtc gctgacacgc ccgaaacgtg ccacgaccgt ttcaatatcg
     1801 ccgagctgct ccgaccaggt agcaaagcag ctgtcgagac gcgcacctgc ggcttccgga
     1861 acgttgctgc gcgttcgcca gtcgacaaaa cggtcctgcg ccaccgcggc cgcttcctgc
     1921 gtctcgcgct caatcacctc gcgtgtaact cctgcggcaa gaatcacgtc gggaaggtag
     1981 gcaaaggcgg tatctggatt cggctgtata aacagcacgc gaatatggga atttagccgc
     2041 ttggccacca agagagcagc gtccagaagg gtttcgctcg acagctctgc ggtcagcggg
     2101 accaagatgg tgcgaatcat ttgcccgacc tctccactct ttgtgagcgg cgagctgtta
     2161 agcgctatct cagggcagca ccttgatcca ggtcaatgct gtgtagaaac ttcgccggcc
     2221 gaggggtgcc gcgtatcact cgtagcgcag tgcctcgatc ggatcgaggt gggcagcttt
     2281 ccgcgccggg taatagccga aaaacacgcc caccgccgcc gcgaaaagga agccgccggc
     2341 gatcgcggcc ggcgagaccg gcgcccgcca gccgaaaaca aacgagatgc cagcagagaa
     2401 ggcgacgccc atggcgatac cggcaatgcc gccgctcacg ctgaggaaca cggcttcagc
     2461 gagaaattga aggaggacgt gcaggcgtcg cgcgccgat
//
LOCUS       DATLJT010001014         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1907340,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001014 DATLJT010000000
VERSION     DATLJT010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907340"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VM910_23315"
     CDS             complement(<1..1488)
                     /locus_tag="VM910_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVI65484.1"
                     /translation="MKPAAKSPAELLAPGRALTIANVAEGAEGLVVSDLARAIAAKPK
                     RAAISLAVICRDGPRMQQLARALEFFAPDLPVMQFPAWDCQPYDRVSPHQGILAQRMT
                     TLARLSRLAGHDKPLIVLTTVNAAVQRVPARDVVAAQALSVAPGHVVPMDSIIAWLEH
                     NGYTRSSTVREPGEYAVRGGILDLFPAGLDQPVRFDFFGDSLESIRSFDAETQRTLLD
                     MRSLDLVPISEFQLVTETIRRFRMGYTAEFGAPDRDDQLYQAVTEGRRHPGMEHWLPL
                     FQERMDTLFDYLGDAVVAIEPQGEDAARERFKQIADYYEARRDALEHRNTGAIYHPLP
                     PDRLYLTEDEWQKRLGEAPLARFTPFAVPEHANVFDAGARQGRNFAPERNDSAVNVFE
                     AVVAHIRSLQARRKKVVVALWSEGSRDRMASMLSDHRLANIVSVNAWRTVQATPRNEA
                     MLAVLGMESGFETDDVAVISEQDILGDRLVRPRKATRKLDNFISEV"
     gene            complement(1485..1775)
                     /locus_tag="VM910_23320"
     CDS             complement(1485..1775)
                     /locus_tag="VM910_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HVI65485.1"
                     /translation="MTGTTRSSVGLDNRRKRLLFRCWHRGTREMDLILGRFADAEIAT
                     LADDELDELERLIEVPDPDLYAALTGARPLPQGYAPALLDRIRAFRAPGTDI"
     gene            1936..>2304
                     /locus_tag="VM910_23325"
     CDS             1936..>2304
                     /locus_tag="VM910_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVI65486.1"
                     /translation="MRPALLNPLFAPVTSLPGVGPKQDKLLRYLLSRDETPRLVDLLL
                     HLPSQVIDRRSRPKICDAVPGEVVTLEVTVDRHRPPPPHNRRAPYLVYASDDTGDVVL
                     TYFRSPPGYVEKLLPVGAKRY"
BASE COUNT          425 a          721 c          750 g          408 t
ORIGIN      
        1 aacttccgag atgaaattat cgagcttgcg ggtcgccttg cgcggccgca cgagacggtc
       61 gcccagaatg tcctgctcgc tgatgacggc aacgtcgtcg gtctcgaaac cgctttccat
      121 gccgagcacc gccagcatgg cctcattgcg cggcgttgcc tgcacggtgc gccatgcgtt
      181 gacgctcacg atattggcaa gcctgtggtc ggaaagcatg ctcgccatgc ggtcgcgcga
      241 accttcgctc cacagcgcga cgaccacctt cttgcgccgc gcctgaagag aacggatgtg
      301 cgcgacgacg gcttcgaaca cgttgaccgc gctgtcgttg cgctctggcg cgaaattgcg
      361 tccctgccgc gcgcccgcgt cgaacacgtt ggcatgctcc ggcacggcga atggcgtgaa
      421 ccgtgcgagc ggagcctcac ccagacgctt ttgccattcg tcttcggtca ggtacaggcg
      481 gtcgggaggc aatggatggt agatcgcacc agtgttccga tgttcgagcg cgtcgcgccg
      541 tgcctcgtaa tagtcggcga tctgcttgaa gcgctcgcgt gccgcgtctt caccctgcgg
      601 ttcgatcgca accacggcat cgccgaggta atcgaacagc gtgtccatcc tctcctggaa
      661 cagcggcagc caatgttcca tgcccgggtg gcgccgtccc tccgtgacag cttgatagag
      721 ctggtcgtcg cgatcgggtg cgccgaactc ggccgtgtag cccatccgga agcggcgaat
      781 ggtctcggtg acgagctgga attccgatat cggcacgaga tcgagcgagc gcatgtccag
      841 gagcgtgcgc tgcgtttcgg cgtcgaagga acggatcgat tccagggaat cgccgaagaa
      901 gtcaaagcgc acgggctgat cgagaccagc cggaaacagg tcgaggatgc cgccacgcac
      961 ggcatactcg ccgggctcac gcacggtcga cgagcgcgtg taaccattat gctcgagcca
     1021 tgcgatgatg gagtccatcg gcaccacgtg gccgggcgcg accgacagcg cctgcgcggc
     1081 gacaacgtcg cgcgcaggca cgcgctgcac ggccgcgttc accgtggtga gcacgatcag
     1141 cggcttgtcg tggcctgcaa gccgcgacag ccgtgccagc gtcgtcatgc gctgcgccag
     1201 gatgccctga tgcggcgaga cgcgatcgta aggctggcag tcccaggccg ggaattgcat
     1261 caccggcagg tcgggtgcga aaaattccag cgcgcgggcc aattgctgca tgcgcggccc
     1321 gtcgcgacag atcaccgcaa ggcttatggc agctcgcttc ggctttgccg cgatggcgcg
     1381 ggcgagatcg gagacgacca ggccctccgc gccttcagcg acattggcaa tggtcagagc
     1441 gcggccaggc gcgagcaatt cagcgggcga tttggcggcc ggcttcatat gtcggtgccc
     1501 ggggcacgga acgccctgat gcggtcgagc agtgcaggcg catagccttg tggaagcggt
     1561 ctggcgccgg tcagcgccgc atagagatcg ggatcgggca cctcgatcag gcgttcgagc
     1621 tcatccaact cgtcgtcggc aagtgttgcg atctcggcat ctgcaaagcg gccgaggatt
     1681 aagtccatct cgcgggtgcc gcggtgccag cagcgaaaca acagccgctt gcggcggttg
     1741 tcgaggccaa cgcttgatcg agtcgttccg gtcatttcac gaaaatccag tacaacgcca
     1801 aaagcccgga cgtgccgggc ggggttgata taacgagaga gatggccgat gtcagccatt
     1861 ccctttcaga aaaaagcgtg gatgttcggc aattaagcgg ggcatcggga cgtcgaaccg
     1921 ttttaaaatt tcaaaatgcg ccctgctctg ctcaatccgc tgtttgcgcc ggtcacgagc
     1981 cttcccggcg tcgggccgaa gcaggacaaa ttgctgcgct atctgctcag ccgtgatgag
     2041 acgccccggc tggtcgatct gctcctgcac ctgccgagcc aggtcatcga ccgcaggtcg
     2101 cggccgaaaa tctgcgacgc ggtgccggga gaggtggtga cgctggaagt aaccgtcgac
     2161 cggcatcggc cgcccccacc gcacaacagg cgcgcgccgt acctggtcta cgccagcgat
     2221 gacaccggcg atgtcgtgct gacctatttt cgcagcccac ccggttatgt cgagaagctg
     2281 ttgccggtcg gcgccaagcg ctac
//
LOCUS       DATLJT010001015         7876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2597162,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001015 DATLJT010000000
VERSION     DATLJT010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597162"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1531)
                     /locus_tag="VM910_23330"
     CDS             complement(305..1531)
                     /locus_tag="VM910_23330"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI65487.1"
                     /translation="MTPLQDIRVLSVTVYLAGPFLSMTLARFGAEVIKVEAPGSGDPA
                     RNLGPFAGPRGIAHDRGADDVSIPFLKRSEGVKCVTLNLKDAEGRRMFLDLAKKTDVL
                     IENLAPGSMSRLGLGYGDVSAVNPGIVYCSIAGYGQSGPYKDLPAHDHQIQAMSGVMD
                     MNGDPDGPPMRVGLYVADLVTPLYAALGIYGALRVKERTGKGQHIDASMIDTLAALMF
                     MEPVEFVAAQGLPVRVGNRARDDVTGLYRLKDGDVFITIVNEGRWQRLCNTIGVAELI
                     DDPRYATVALRKERLPELRDIVQARLGRLTCEQGIALLEKAEIPVSRVRGLSEALHDE
                     HFRARGTLRPMRRSGSTAPVEGGVLPGLPLRFSGGELPQLDGGADLGQHNAEVYGRLL
                     SLDAAALGALKARGVV"
     gene            complement(1694..2569)
                     /locus_tag="VM910_23335"
     CDS             complement(1694..2569)
                     /locus_tag="VM910_23335"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVI65488.1"
                     /translation="MKPIRSMMFVPGNKPSMIEKAAKAKADALILDLEDSVPPAERLK
                     ARELIVSRLSWLAEQRQRIYVRINRSPYLYDFDDMLAVVGPHVEGIVLSKPNGPEDVD
                     CISLMLNEVEHRKGMAIGATRLVPTLETARSMELVYEIAKRDRVTALGGAAARSADTE
                     RALGYVWSADGREVLYLKSRVVMAARAAGKAPLGGLWQQVHDIEGLRAFLARDRQLGM
                     TGVFLLHPSNVAPANETFSPSAAEIAYYEGMIDAHAAAVAAGRASCIYDGEHIDIAHV
                     ETARQIVAMAASVEN"
     gene            3086..4324
                     /locus_tag="VM910_23340"
     CDS             3086..4324
                     /locus_tag="VM910_23340"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI65489.1"
                     /translation="MTGVYEGLTVIELADRRNQWAGKLLSDGGARVIQVEPIAGSPGR
                     WCGPFADDKPDPDLCIDYWWNNTGKQSAAVDVARPAGRDLLKQLLAKADVFLESTPPG
                     TLANLGLGYDAAASNRNLIYASLTDFGQDGPWRDYVANDVAHLALGGQMASSGYSDPK
                     ETPIGGKGHHAWHMGCAFMLHGITAALIERMGSGEGQFIDVSIHDACAIGTEAAVPQW
                     MYYGSTMYRQTGMHANPKRQPPLQLPTADGKYMIAVMQNFGRPVWEKLIKWMDEKGVT
                     GELHDPKYQDEAFRMSEYRTGNVIRDAIARLIAASDGEECFHRAQSYNISWGLVHAAE
                     ENYDVAHYRQRDYWRSIEHPQIGRAIPYPRGPFACDALRIEPRGRAPNLGEHTQHVLA
                     HDLGMSADAIMALRTSGVVR"
     gene            4321..5688
                     /locus_tag="VM910_23345"
     CDS             4321..5688
                     /locus_tag="VM910_23345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI65490.1"
                     /translation="MNTTSTARPGVLSGVRVLDFTWKTVGPWSTRLLTHYGAEVIHVE
                     RAGGWDDHRYNAAPSIVTDEPKVAQTLGMKKVYSDPQFNTHRDESAAATERPKLYAAP
                     YFNTLHNGKLAISLNTKSPEGLKIVERLIGMCDALVENFSAEVLPSWGLTWERLHAIN
                     PRLVYMSTSGFGHTGDWKTYRSYGPTAAAQSGLSLASGLPGKPPAGWGFSYLDVMGGW
                     MGGLSLIQGLLQAKKTGEGCHIDYSVTEGAMSMLGTYMLDYQVNGRRTRRPDFPPGNR
                     AVFPALAPHNTYRCAGRDRNGQDWWIFIACETQVQFEALCSVMEQPGLVLDTKFATNE
                     ARVTNQAELDQIISNWTRPRRRYDIMRKCQAAGIVAAVVQNAEDRVEYDPQLQHREVF
                     PVIENPEVGGAFSYERYPVRLSRTPATVQSRAPVLAEHNQYVFGELLQMSEAEINSLR
                     ERGVI"
     gene            5811..7007
                     /locus_tag="VM910_23350"
     CDS             5811..7007
                     /locus_tag="VM910_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI65491.1"
                     /translation="MTPSPLAFGGRHPNPRNKRAAAVIGVGHTDWVRDWQRCRAGEQP
                     NDSLGYGLEAFRNALKDANINRDEIDGLIAGPTTAYERMGELAGINPRWGNQADAVLA
                     VSQAVMAIEAGYAEVVALIYGNDQRSVGTQYGGPAAMGGNSFLSYVYHSPWGFTSQGA
                     LYALTLQSYKHSRGFTEREMGEVSVAQRGWSSLNPNAIQRKRISIADYLASHYVCQPL
                     HLFDYCLINDGGVALIIAEAERAKKLSSRPVFIEGIGRSDLNRGATSLEPRIDEFYLS
                     AQQAAAAQSYAVSGLGPKDMDVLQVYDSFSVHVPLALEGYGYAKVGEAGKFLRESGIG
                     PGGKLPTNTSGGHLSETYMQGWAHQVECVRQLRGECGDRQVKDARHVHYTSDVAGKCF
                     SVIYGV"
     gene            7013..7423
                     /locus_tag="VM910_23355"
     CDS             7013..7423
                     /locus_tag="VM910_23355"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVI65492.1"
                     /translation="MNATEVKTPRPVMGVYDKPMWESIRAGAMKLQRCKHCATWLYPP
                     GPSCPKCLSPELEWTPVSGEGKIVSWVVFHRHYLPAYPPPYNAIAVRLAEGPTMISNL
                     EGAMPEDGCIGRAMRLIYVTMPDGVMLPRWRYEG"
BASE COUNT         1499 a         2406 c         2510 g         1461 t
ORIGIN      
        1 atggaatgat gaacgggtga ggccacgaaa aggaccccgt tctgcctcca agggtgaggg
       61 cccgctacca gccagagaag agaccttcgc gggagtgcgc tgcgacggcg ggtcagcgcc
      121 ggaagcggtc attccgccgg cgcgatcaag ccgcgatttc gtcgaactcg atggtttccg
      181 gcgtttggtg gacctgcccg ccgagactgg gaaggccccg gccgttgctg accgacccga
      241 tcgccaactt acaatgtgat cttcccccga aaaagtggac ggggggttgc gctagtggct
      301 cggctcagac cacgccgcgt gctttcagtg ccccaagcgc cgccgcatcc aagctgagaa
      361 ggcggccgta gacctcggca ttgtgttgac caagatcggc gccgccgtcg agttggggaa
      421 gctcgccacc ggagaacctg agcggtaggc cgggaaggac gccaccttcg accggagccg
      481 tgctgccgga gcgtcgcatg ggccgcaagg ttccgcgtgc cctgaaatgc tcgtcgtgca
      541 acgcttccga caatccacgc acgcgcgaga ccgggatttc ggctttctcc agcagggcga
      601 tcccttgttc acaggtgagg cgcccaaggc gcgcctgaac gatgtccctc agctctggca
      661 gacgctcttt ccggagagcc accgtcgcgt agcgtggatc gtcgataagt tctgcaacgc
      721 caatcgtgtt gcagaggcgc tgccaccgcc cctcattcac gatcgtgatg aaaacatcac
      781 catccttcaa tcgatagagc cccgtcacat cgtcgcgcgc cctattgccg acgcgtaccg
      841 gcagaccctg cgccgcgacg aattcgacgg gctccatgaa catcagcgcg gccagcgtgt
      901 cgatcatcga agcgtcgatg tgctggccct ttcccgtccg ttccttcact ctgagtgcgc
      961 cgtagatgcc gagcgccgcg tagagcggcg tgaccagatc cgcgacgtaa aggccaaccc
     1021 gcatcggtgg cccgtccgga tcgccattca tgtccatgac gcccgacatc gcctggatct
     1081 ggtggtcgtg cgcaggcaga tccttgtagg gaccagattg gccgtaaccg gcgatcgagc
     1141 aatagacgat gccgggattg acggccgaga catcgccgta gccgaggccc agccgcgaca
     1201 tcgatccagg cgccagattt tcgatcaaca catcggtttt ctttgcaaga tcgaggaaca
     1261 tgcggcgccc ctcggcatcc ttgaggttca gcgtcacgca cttgacgccc tcggagcgct
     1321 tgaggaacgg gatcgagacg tcgtcggcgc cacggtcgtg cgcgatgccc ctggggccgg
     1381 cgaaggggcc gaggttgcgc gcggggtcac ccgagcctgg tgcttcgacc ttgatcacct
     1441 cggcgccgaa tcgggcaagt gtcattgaaa gaaacgggcc ggcgagatag acggtcacgg
     1501 acagcacgcg tatgtcttga agtggcgtca atggaaactc ctcggtttgg gagtgcagct
     1561 gcaggttcag attgactgcg ccgaatatgc tgtcgagaga ccaccactaa gctatctgaa
     1621 gcaacttcac ccaatgaatc ccttatcttt gccaacgatg ttttagaaca agatcgcgcg
     1681 ctcatccgcg cgatcagttc tcgacagacg cagccattgc tacgatctgg cgcgcggtct
     1741 caacgtgggc gatgtcgatg tgctcgccgt cgtaaatgca ggatgcgcgg cccgccgcga
     1801 ccgccgcggc gtgggcgtcg atcatgcctt cgtaataggc gatctctgcc gctgacggac
     1861 tgaacgtctc gttcgcgggg gcgacattcg acgggtgcag caggaacacc ccggtcatgc
     1921 cgagttggcg atctcgcgcc aggaaggcac gcagtccctc gatgtcgtgt acctgttgcc
     1981 agaggccgcc caacggcgcc ttgccggccg ctcgcgccgc catcaccacg cgcgatttca
     2041 gatagagcac ctctcgccca tccgccgacc agacgtagcc gagcgcgcgt tcggtatcgg
     2101 cactccgcgc agcggcgcca cccaaggctg tcacgcggtc acgcttggcg atctcgtaga
     2161 ccaattccat cgaccgcgcg gtctcgagcg tcggaacgag ccgggtggct ccaatcgcca
     2221 tgccctttcg gtgttccacc tcgttcagca tcagcgaaat gcagtcgaca tcctcaggtc
     2281 cgttgggctt cgagaggacg atcccctcca catgcggccc gacaacggcg agcatgtcat
     2341 cgaaatcata gaggtaggga ctgcggttga tgcggacgta gatgcgctgc cgctgctccg
     2401 ccagccagga gagcctggaa acgatgagtt cccgcgcctt gaggcgctcg gccggcggta
     2461 cgctgtcctc gagatcgagg atgagcgcat cggccttcgc cttcgcggcc ttctcgatca
     2521 tagagggctt gttgccgggc acgaacatca tcgatctgat gggtttcatc tggaactccg
     2581 tcacgcgtgc gagaaggcaa caatgtcaca ggaagttgga gcacgcttgc tcattccggc
     2641 tggttgagca gccgacaaaa ttcgacttcg tgatcaaacc ttaagactgc gaaggcgctc
     2701 gggcttaccg tgccgccagc attgctcgcc cgcgccgacg aggttatcga gtaaggcaga
     2761 agagcgcccg ctttggggca tcttgagcgg ttcccgtccg agagggtgag cgcccgctat
     2821 gccatcagat aggaggcggt cgccagaacg tccggctacg acccccatgc gccgtaagcg
     2881 gacgtcgcat cgcggaaagt ggcgtccgct gctcggcgca acgggtccgc ccacgcagcg
     2941 cgatctccat gtggcgatgg tgacggaaca cggtcgactg ggttggcagg cggctactgg
     3001 ctatggcacg cttttccgcc gatcccggcg ccggggtggc tcagccgccc tcaacgcgac
     3061 gtgcggtttt gcaaaaggaa acagtatgac gggcgtctac gagggtctca cggtgatcga
     3121 gctggccgat cgccgtaatc aatgggcagg taaactgctg tcggacggtg gcgcccgagt
     3181 catccaggtc gagccgatcg cgggtagtcc gggccgctgg tgcggcccgt ttgctgacga
     3241 caagcccgac cctgaccttt gcatcgacta ctggtggaat aacaccggca agcagagtgc
     3301 ggcagtcgac gtcgcacggc cggcaggccg cgatctgttg aagcaactgc tggcgaaagc
     3361 ggacgtcttt ctggagagca cgccgccagg gacgctcgcg aatctcggcc tcggctatga
     3421 tgccgccgca tcgaaccgca acctgatcta cgcctcgctc accgacttcg gccaggacgg
     3481 tccctggcgt gactatgtcg cgaacgatgt cgcccatctt gcccttggcg gacagatggc
     3541 gagttcgggc tactccgatc caaaggagac gccgatcggt ggcaaaggcc accatgcttg
     3601 gcacatgggc tgtgccttca tgcttcacgg tatcacagcc gccttgatcg agcgcatggg
     3661 ctctggcgaa ggccagttca tcgatgtgtc gatacacgac gcatgcgcca tcggcaccga
     3721 ggcggcggtt ccgcagtgga tgtattacgg ttcgacgatg tatcgccaga cgggcatgca
     3781 cgccaatccg aagcggcagc ctccgttgca gctgccgaca gccgacggca agtacatgat
     3841 tgccgtgatg cagaattttg gtcggccggt gtgggagaag ctgatcaagt ggatggacga
     3901 gaagggcgtg acaggcgagt tgcacgatcc gaagtaccag gacgaggcgt ttcgtatgtc
     3961 ggaataccgg acgggcaacg tcatacgtga cgccatcgcg cgtctgatcg ccgccagcga
     4021 tggcgaagag tgcttccatc gcgcgcagtc gtacaacatc tcgtgggggc tggttcacgc
     4081 cgccgaagag aattacgacg tcgcgcatta ccggcagcgc gactattggc gctctatcga
     4141 gcatccgcag attggccgtg cgatcccgta tccacgcggt ccgttcgcgt gtgatgcctt
     4201 gcgaattgag ccgcgcggcc gcgcgccgaa tcttggcgaa cacacccagc acgtattggc
     4261 gcacgatctt ggcatgtcgg ctgatgcaat catggcactg cgcacctcgg gggtcgtccg
     4321 atgaacacta cgtcaactgc ccgtcctggt gtgctcagcg gtgtgcgcgt tctcgatttc
     4381 acctggaaga ccgtaggtcc gtggtcgacg cgcctgctca cgcattacgg cgccgaagtc
     4441 atccatgtcg agcgtgccgg aggctgggac gatcaccgct acaacgcggc tccgagcatc
     4501 gtgaccgatg agcccaaggt ggctcaaacg ctgggcatga agaaggtcta ctccgatccc
     4561 caattcaaca cgcatcgaga cgagtcggcg gctgccaccg aacggccgaa actctacgct
     4621 gcgccttact tcaatacgct acacaatgga aagctcgcca tcagtctcaa caccaaaagt
     4681 ccagaaggat tgaagatcgt cgagcgtctc atcggcatgt gcgatgcgct ggtggaaaac
     4741 ttcagtgccg aggtgttgcc gagctggggc cttacctggg agcgactcca cgccatcaat
     4801 cctcgactcg tttacatgag tacgtcgggt tttgggcaca cgggcgattg gaagacctat
     4861 cgcagctacg ggccgacggc cgccgcacaa tccggtctgt cgctggcctc gggcttgccc
     4921 ggcaagccgc ccgcgggctg gggattctcg tatctcgatg tcatgggcgg ctggatgggc
     4981 gggctttcgc tgatccaggg tctgctgcaa gcgaagaaga cgggtgaggg ttgccatatc
     5041 gactactcgg tcaccgaagg ggcgatgtcg atgctaggca cctacatgct agattaccaa
     5101 gttaacgggc gacgcacccg tcggccggat tttccgccag gcaatcgcgc ggtgtttccg
     5161 gccctggctc cgcacaatac gtatcgttgc gcgggcaggg accgtaatgg tcaggattgg
     5221 tggatcttca tcgcgtgcga gacccaggta cagttcgaag ccttgtgctc tgtgatggaa
     5281 cagccgggtc ttgtccttga tactaaattc gccaccaacg aggcgcgggt cacaaatcag
     5341 gctgagctcg atcagatcat cagcaactgg acgcgcccgc ggcgacgcta cgacatcatg
     5401 cggaaatgtc aggcggccgg cattgtcgcc gccgtcgtgc agaatgcgga agatcgtgtg
     5461 gaatacgatc cgcagttgca gcatcgcgag gtgtttcccg tcatagagaa ccccgaagtc
     5521 ggcggcgcat tctcttatga acggtaccct gtgcggctgt cgcgtacgcc ggcaaccgtc
     5581 cagagccgtg cgcctgtgct tgcggagcat aatcaatatg tcttcggcga gcttctgcag
     5641 atgagcgagg cagaaatcaa ttcgttgcgg gagcgcggtg tgatctaggg gcgcgtcgct
     5701 cggcggtcct cgtgattgta caaggcggcc aaatgcttgc gttccgaatt catggaaatt
     5761 gacatgggcc gcgaggaaat cgtggtgctc acggagagga tgatcctggc atgacacctt
     5821 cacccctggc cttcggcggt cgccacccaa acccgcgcaa caagcgcgcc gccgctgtca
     5881 tcggcgtcgg ccacaccgac tgggtccgcg actggcagcg ctgccgcgcc ggcgagcagc
     5941 caaacgattc cttaggctat ggcctcgagg cgttccgtaa cgcgctgaag gacgcgaaca
     6001 tcaaccgcga cgagatcgat ggcctgatcg ccggtcccac caccgcctat gaacgcatgg
     6061 gcgaactcgc gggcatcaat ccgcgctggg gcaaccaggc ggatgccgta ctggccgtct
     6121 cgcaggctgt tatggcgatc gaggccggct atgccgaagt cgtggcgctc atctacggta
     6181 acgaccagcg cagtgtcggc acgcagtatg gcgggccggc ggcgatgggc ggcaactcct
     6241 tcctctctta tgtctatcac tcaccctggg gcttcacttc tcagggcgcg ctctatgcgc
     6301 tgacactgca gagttacaag cactcacgcg gcttcacgga acgcgaaatg ggcgaggtct
     6361 cggtcgcgca gcgcggctgg tcatcactga acccgaacgc gatccagcgc aagcgcatct
     6421 cgatcgcgga ctatcttgct tcccattatg tctgccagcc gctgcatttg ttcgactact
     6481 gcctgatcaa cgatggcggc gtcgcgctga tcatcgcgga ggcggaacgg gcaaagaagc
     6541 tgagcagcag gccggtgttt atcgagggca tcgggcgctc cgacctcaac cgtggtgcta
     6601 ccagcctcga gccgcgcatc gacgagttct atttgtccgc gcagcaggct gccgcagcgc
     6661 aatcctatgc cgtatcgggt ctcggtccga aggatatgga cgtcctgcag gtctatgaca
     6721 gcttctccgt ccacgtgccc ctggcgctgg aaggttatgg ctacgccaag gtcggcgagg
     6781 ccggaaagtt tctgcgcgag agcgggatcg ggccaggcgg caaattgccg accaacacca
     6841 gcggcggaca tctcagcgag acctacatgc agggctgggc gcaccaggtc gaatgcgtgc
     6901 gtcaactgcg cggcgagtgc ggggaccgtc aggtgaagga cgcgcggcac gtgcactaca
     6961 cttccgacgt tgccggcaag tgtttctcgg tcatctacgg agtgtgaacg tcatgaatgc
     7021 gaccgaagtc aaaacgccgc ggcccgtgat gggcgtttac gacaagccga tgtgggaaag
     7081 tattcgcgcc ggtgcgatga aactgcagcg ctgcaagcac tgcgcgacct ggctttaccc
     7141 gcccggtccg tcctgtccga aatgcctctc tcccgaactc gaatggacgc ctgtttcggg
     7201 cgaggggaag atcgtctcct gggtggtgtt ccaccgtcac tatctgccgg cctatccgcc
     7261 gccctacaac gcgatcgcgg tgcggctcgc ggaagggccg acgatgatca gcaatctcga
     7321 aggcgcgatg ccggaggatg gctgcatcgg acgcgcgatg cggctgatct atgtgacgat
     7381 gcccgacggc gtgatgctgc cgcgctggcg ctacgaaggc tgaactgtgc cggcgcccag
     7441 aacacgccga gataccaaag ggagcgtagt ttgaccgtat gaccgcatgt ccgtgccgat
     7501 gatggtggct cgtcccatac tgcaagatca gcgaggttgc tcgcatgtgg cgggcctttc
     7561 ctcagcgcgg gggacgagcc gtggacgagc atagcgaaat ttatgttggg ctggacgtag
     7621 cgatagcccg gcacgcggtg gcggtggctg acggcgagcg atcgggcttc cgggttgcga
     7681 ccctcgtcgg ccggtttgcc actcgtctca gaaggtcggc ttaggggcat gggcagaagg
     7741 ccatgcgatg agcgcgatcg gcgggttccg gatcggtcac ccgacctttg ccggagcatc
     7801 cagcgcgggg gcgcgggggc ccggcgccga atgcatattg catccagcgg cgctgttcag
     7861 gaccgcaaaa cgacgt
//
LOCUS       DATLJT010001016         7136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1907347,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001016 DATLJT010000000
VERSION     DATLJT010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907347"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VM910_23360"
     CDS             <1..1233
                     /locus_tag="VM910_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVI65493.1"
                     /translation="HAASFALLVYASAWLKCRYPDVFAAALLNAQPMGFYAPAQIVRD
                     LREHGVEVRPVDVNYSHWDATLEPGPHAAARVHPLHREMAGDIRTTHALRLGFRKIKG
                     LSEEHGRSIVQNRAAGYTCLRDLWLRTGLSPRIIERLADADAFGSLGLSRRQALWAAK
                     ALGRVGDKDDDLPLFRGQAMQAPVTAAPPPAPISVPREPAVPLPPMSLGEEVVNDYRF
                     LELSLRPHPACFLRADLGRRGIIRNEELRVQPSGARVSVSGLVTVRQQPGSANGVIFM
                     TIEDETAVANIIVWPKTFERFRPIIMGARYVAVSGELQQESGVIHVVAAKLNDLTALL
                     ACLTEDAPPIEALARADAVKRPHDENVDSRARGRRNPPRIAAPAQLPDLLASDLDVPA
                     RGSAHAPARRGGTKIVRR"
     gene            complement(1383..2366)
                     /locus_tag="VM910_23365"
     CDS             complement(1383..2366)
                     /locus_tag="VM910_23365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI65494.1"
                     /translation="MKRGRLIVVAGQTTAALSRRRFLTGTAAGAIALGTGTIIPARPG
                     WANAQPVYRSGDLEALVVSDGHFVLPTGFLVTPEAPLAEREAVLKATGQGGEQIQLVN
                     NVTVIRKQSEVILVDAGTGPRHQPTAGKLAENLRAAGIQPAAVTKVVLTHGHPDHLWG
                     VLDANDNPIYPNASYIISAVEWDVWSDPDVVHKLPAAMPKEQIAKGAKNHFSHIKDKV
                     VMVREGYEIVSGVRVIDTPGHTPGHMSVEVAGGDGLVIAGDALTHVLISFRHPSWKVP
                     VDHEADRGISTRLRLLDRLAVDKRRLVAAHLPFPGTGFVDRKDGAYRFVAS"
     gene            complement(2908..3684)
                     /locus_tag="VM910_23370"
     CDS             complement(2908..3684)
                     /locus_tag="VM910_23370"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI65495.1"
                     /translation="MGTLATFDPKDLEARVKAMYRKVAEDPHGEFHFEMGRAMAERLG
                     YAPADLDRIPAEAIESFAGVGYYFDLAALKEGETVVDLGSGSGMDTLVAALKVGKKGQ
                     VIGIDMTDAQLDKAERLRAAAAFSNVAYRKGYIDRTSCADASADVVISNGVINLAPDK
                     ASVFREAARLLKSGGRLAIADIVTDAQLPETITCNADLWAACIGGAMQRDDYRTAIEA
                     AGLKVKAVRENAGYLFISANARGAQQKWGVKSISLLAVKV"
     gene            complement(3801..4169)
                     /locus_tag="VM910_23375"
     CDS             complement(3801..4169)
                     /locus_tag="VM910_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HVI65496.1"
                     /translation="MAEPASELVVIITHGADHELSSVAFTIANGGITSGLKVSIFLTS
                     NGVDLVRKRAIDNLVVHPLEPLAALVKDFLARGGRLWACTPCVKARGYEQKDLIDGVE
                     VAGSSVMHERIKAGAATLSF"
     gene            complement(4150..4722)
                     /locus_tag="VM910_23380"
     CDS             complement(4150..4722)
                     /locus_tag="VM910_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65497.1"
                     /translation="MKAALQTLAIGVVAVASVAPTASAQTMEGHKIVSAQEIEWGPAP
                     PSIPPGAQAAVLYGDPGKEGLFSLRLKLPKDYAIPPHTHPKPEVVTVISGTFRLGMGE
                     KADKSKGQALPAGSFFALSPGLAHFAFADEDTVIQLNSTGPWGLTYVNPADDPRKKRA
                     AQQALKKAALLLVPGRNLQTQGMTYGRTRQ"
     gene            4864..6483
                     /locus_tag="VM910_23385"
     CDS             4864..6483
                     /locus_tag="VM910_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI65498.1"
                     /translation="MRFLFGDCILDPTRRELTRAGEAIHVEPQVFDVLLHLIRNRERV
                     VSKDDLLAAVWGGRIVSESTLNSRINTARRIIGDSGEHQHFISTIARRGFRFVGDVRQ
                     ERIDSKRNDESLVPAVAPTEPEQPYGLEGDRQTSLGERFGGKPTVVVLPFNNMSGDPG
                     QEYLSDGITEDIITVLSKHRSLLVIARNSAFSFKGHSGDVRRIGTELRADYIVEGSVR
                     KIGLGVRITAHLIETEGGQLVWAEQYDRELEEIFELQDEITRTIAARIEPGVGSAERL
                     RAERKPREALQAWDLFHLGLVHFYKFAAEDNREAQQLLRRAASLDPKLAQAFAFLSYA
                     IVLSMLYFDAKADDESLNEAESLAKKGVELDDQDALIRFIHGRVLLARQAYGDALAEL
                     ETALELNPSLPAVYCGLGDTLAYEGRYAQAIPHFERAINLSPYDPQRWAFYSYGALAQ
                     IFAGDFDRAFGWAQRAIRIPNCHYWPFAHRVAALGHMQREEELRIAVAELLRRKPGFS
                     CGFARKRLFYVKNPLQLNRYVEGLRKAGIPE"
BASE COUNT         1270 a         2241 c         2256 g         1369 t
ORIGIN      
        1 cacgcggcga gcttcgcgct cctcgtttac gcttcggcct ggctcaagtg ccgctacccg
       61 gacgtgttcg cggcggcgct gctcaatgcc caacccatgg gcttctatgc gccggcgcag
      121 atcgtgcgcg acctgcgcga gcatggtgtc gaagttcgcc cggtcgacgt caactattcc
      181 cactgggacg ccacgctcga gcccggcccg cacgcagccg cgcgcgtgca cccgctccac
      241 cgcgagatgg cgggcgacat ccgcaccacc cacgcgctgc ggctgggttt tcgcaagatc
      301 aagggattgt cggaagagca cggcagaagc atcgtgcaga accgcgcagc cggctacacc
      361 tgcctccgcg atctctggct gcgcactgga ctttcgcccc gcatcatcga gcggctcgcc
      421 gatgccgatg ccttcggctc gcttggtctc agccggcggc aggccttgtg ggcggcgaag
      481 gcgctcggcc gcgtgggcga caaggacgat gatctgccgt tgtttcgcgg gcaggcgatg
      541 caagcgcccg tgacggccgc tccgccgcca gcgcccattt cagtcccgcg cgagcccgcc
      601 gtgccgctgc cgcccatgtc gctgggcgaa gaggtcgtga acgactaccg cttcctcgaa
      661 ctgtcgctgc gcccgcatcc ggcgtgcttc ctgcgcgccg acctcggccg ccgcggcatc
      721 atccgcaatg aggagctacg cgtgcagccg tcgggtgcgc gcgtcagcgt ctccggcctc
      781 gtcaccgttc gccagcagcc gggctcggcc aatggcgtca tcttcatgac catcgaagac
      841 gagaccgcgg tcgccaacat catcgtctgg ccgaagacgt tcgagcgctt tcgtcccatt
      901 atcatgggcg cgcgctatgt cgccgtgagc ggagagctgc agcaggaatc cggagtcatc
      961 cacgtcgtcg cggcaaagct caacgatctc accgcgctgc tcgcgtgctt gaccgaggat
     1021 gcgcctccga tcgaggcgct cgcgcgcgcc gacgcggtca agcgcccaca cgacgaaaat
     1081 gtcgacagcc gcgcccgcgg ccgccgcaat ccgccgcgta tcgccgcgcc cgcgcaactt
     1141 ccggatttgc tggcaagcga tctcgatgtt cccgcgcgcg gctcggcgca cgcgccggcg
     1201 cgccgcggcg gcacgaaaat cgtgcgccgc taagcagacc gtgagtaatg ctcgacgtca
     1261 tgagtcaatc gcgcctgggc atgtgtaaac cgcgcaaccg ccttcggcgg gtcagcgtgg
     1321 caaggtggga ctggtccgcc tgcggcgctt aagcgtcttc tattgggcaa tgtcttgaaa
     1381 cttcaactcg cgacaaatcg gtaggctcca tctttgcggt cgacgaaccc agtacccggg
     1441 aacggcagat gagcggcaac aagccgccgt ttgtcgacag cgagccggtc gaggaggcgc
     1501 agacgggtcg atattccgcg gtcggcctca tggtcgactg ggactttcca cgacgggtgc
     1561 cggaatgaga ttaggacatg cgtcagcgcg tcccccgcaa tcactaggcc gtcgccgccc
     1621 gcgacctcta cagacatatg acccggtgta tgtccgggcg tatcaattac gcgcacgccg
     1681 ctgacgatct catagccttc ccgcaccatc acgaccttgt ccttgatgtg cgagaaatgg
     1741 ttcttcgcac ccttggcaat ctgctccttc ggcatcgcag ccggcagctt gtgcaccacg
     1801 tccgggtccg accatacgtc ccactcgacc gccgaaatga tatagctcgc gttcggataa
     1861 atggggttat cgttcgcgtc gaggacgccc cagagatgat cgggatggcc gtgggtcaat
     1921 acaaccttgg tgacggccgc aggctggatg ccggcagctc tgagattttc ggcgagcttc
     1981 cccgccgtgg gttggtgacg cgggccagtg cctgcgtcga ctaggatcac ctctgattgt
     2041 ttgcggatca ccgtgacgtt gttcacgagt tgaatctgtt cgccgccttg cccagttgcc
     2101 ttcagcaccg cctcgcgctc ggccagtgga gcctccggag tgaccaggaa gcccgtcggc
     2161 aagacgaaat gcccatcgct cactactagg gcttcgaggt caccagacct gtagaccggt
     2221 tgcgcgttgg cccatccggg tcgagcggga atgatcgtgc cggtaccgag ggcgatagcg
     2281 cccgccgcgg tgccggtgag aaagcgccgg cggctcagcg cagcggtagt ttgacctgcg
     2341 acgactatca ggcgtcctcg tttcatggtc tgcctccaaa tacctcgtgc tgtcacgcac
     2401 gctcgtccgc gtggacaagc acggttgatc gagacgcgaa ggcgaaaggt ctgccgagag
     2461 tgaagatgcc tgccgacggc atccgcgctg ccgcgaaatt aggagttcgg catctcccga
     2521 tggcagctct ggcggtagat tacatcactt tcgcgtttgc atagaaggga cctgatgtca
     2581 gctcatgggg cactacccac gatgcagact ccttggccga cacatcgagt ccaccccgag
     2641 cgcgtgcgtc ggatcggttt cctgcacgat tatagcgaga ctcgactcgg agggacgggt
     2701 gcaagtcgtc gcatttcgtg aggcgcttca aaagctgggc tggatcgatg gccgcaccgc
     2761 actgacggct tagctcttgg tagtgatgtg ccgttagcga ggctctggga caggatcaga
     2821 aagatctccg agagccgtcc aggggtaccg gcatggcgcc ccggctgcac acgaacgcgc
     2881 acgaaaccaa cgtcgcaaat cgcaggctca cactttcacc gcgagcaagg agatgctctt
     2941 cacgccccac ttctgctgtg cgccccgcgc gtttgccgat atgaagaggt aaccggcatt
     3001 ctcgcgcacg gctttcacct tgaggcccgc agcctcgatc gcggttcggt agtcgtcccg
     3061 ctgcatggcc ccgccgatgc aggcagccca caagtccgca ttgcacgtga tggtctccgg
     3121 caattgcgca tcagtgacaa tgtcggcgat ggccaaccgg ccgccggact tcagaaggcg
     3181 tgctgcctct cggaacacag acgccttgtc gggcgctaga ttaatgacgc cgttgctgat
     3241 gaccacatcg gcgctggcgt ccgcgcacga tgtcctgtcg atgtatccct tgcgataggc
     3301 aacgttggag aaggcggctg ccgcgcgcag gcgctcggcc ttgtccagct gcgcatcggt
     3361 catgtcgatg ccaatcacct gccccttttt gcctaccttc agggcggcga cgagggtgtc
     3421 catgcccgac ccgctgccga gatcgaccac ggtctcgccc tccttgagag cggcgagatc
     3481 gaagtagtag ccgacaccgg cgaaggactc gatcgcttcg gccggaatgc ggtcgagatc
     3541 ggcaggcgca tagccgaggc gctcggccat cgcgcggccc atctcgaaat ggaactcgcc
     3601 gtgcgggtcc tcggcgactt tgcggtacat cgccttgacc ctggcttcga ggtctttggg
     3661 gtcgaaggtc gccaatgtgc ccatgaggtt gctccttaat gcgactgtag gttgggttag
     3721 cgcgtcgcgc gtaacccaac actgggtgcc tcacgattgt tgggttacgg ccttgggccc
     3781 aacccaacct acaatgctcc tcaaaagctg agcgtcgccg cgccggcttt gatgcgctcg
     3841 tgcatcacgc tggagccggc gacctcaacg ccgtcgatga gatccttctg ctcgtagcca
     3901 cgcgccttga cgcagggcgt acaggcccac aggcgaccgc cgcgggcgag aaagtccttc
     3961 accagcgctg ccaggggctc cagcggatgc acgaccaagt tgtcgatggc gcgtttgcgc
     4021 accaggtcga ccccgttgct cgtcaggaag atcgagacct tcagtccaga ggtgatgccg
     4081 ccgttggcga tggtgaaggc caccgacgac agctcgtggt cggccccatg cgtaatgatc
     4141 acaacaagtt cactggcggg ttctgccata tgtcatcccc tgcgtctgga ggttccttcc
     4201 aggcacaagc aagagagccg cttttttgag ggcttgttgt gctgctcgct tcttcctcgg
     4261 atcgtccgcc gggttgacgt aggtgaggcc ccacgggccc gtgctgttga gctggatgac
     4321 cgtgtcctcg tcggcaaagg caaagtgcgc caagccgggc gaaagggcga agaagctgcc
     4381 cgccggcagg gcttgaccct tgctcttgtc ggccttctca cccattccga ggcggaaggt
     4441 gccggagatg acggtgacga cctcgggctt cggatgcgta tgtggcggaa tggcgtagtc
     4501 cttcggcagc ttgaggcgaa gcgagaacag gccctccttg cccggatcgc cgtagagcac
     4561 tgcagcctgc gcgccgggcg gaatcgaggg cggggccggt ccccactcga tctcctgcgc
     4621 cgagacaatc ttgtgcccct ccattgtctg cgccgaagcc gttggagcga cactggctac
     4681 ggcgaccact ccaattgcca aggtttgcag cgctgccttc attggtgacc tccgtatgtg
     4741 cggcagatcc acgcacgcgt gcagactgga gcatctttga gcgtggcgtc tcctgggaga
     4801 tcttgatttt tccaccagat gctcgccatt tttggtctac ctggatcccg aaggaaccgc
     4861 ccggtgcgct ttctgttcgg cgattgcatc ctcgatccga cgcggcgcga actgacgcgc
     4921 gctggcgaag cgatccacgt cgagccgcag gtcttcgatg tgcttctcca cctgatccgc
     4981 aatcgagaac gagttgtcag caaggacgac ctgctggcgg cggtgtgggg cgggcggatt
     5041 gtgtcggagt ccaccttgaa cagccggatc aatactgcac gtcgcatcat cggcgacagc
     5101 ggcgagcacc agcatttcat cagcacgatc gcacgcaggg gattccggtt tgtgggcgat
     5161 gtgcggcaag agcggatcga ctcaaagcgc aacgacgaga gcctcgttcc ggctgtggcg
     5221 ccgaccgagc cggagcagcc ttacgggcta gaaggagata ggcagacgtc gcttggggaa
     5281 cgttttggcg gcaagccgac cgtagtcgtg ctgccattca acaacatgag cggcgatccg
     5341 gggcaggagt acttgtccga cggcatcacg gaggacatta tcaccgtgct gtccaagcac
     5401 cggtcgctgc tggtgatcgc ccgcaactca gcattctcgt tcaagggaca cagcggcgac
     5461 gtacgccgca tcggcacgga gctgcgcgcc gattacatcg tggaaggtag cgttcgcaag
     5521 atcgggctgg gcgtgcggat aacggcgcat ctgatcgaaa ctgaaggcgg ccagctcgtg
     5581 tgggccgagc agtatgatcg cgagctcgag gagattttcg agctgcagga cgagatcacg
     5641 aggaccatcg ctgcgcgcat cgagccgggc gtgggcagcg ctgagcgcct gcgtgcggag
     5701 aggaaacccc gcgaggcatt gcaggcctgg gacttgttcc atctcggact ggtgcacttc
     5761 tacaagttcg cggctgaaga caaccgggag gcgcagcagc tgttacgccg cgccgcgtcg
     5821 ctcgatccga aactggcgca ggctttcgcg tttctttcct acgctatcgt cctcagcatg
     5881 ctctacttcg acgcgaaggc tgacgacgag tcgttgaacg aagccgagag ccttgccaag
     5941 aagggcgtag agctcgatga ccaagacgcg ctcatccgct tcattcatgg tcgcgttctg
     6001 ctggcgcggc aggcctatgg agacgcgctc gccgagctgg agactgccct cgaactcaac
     6061 cccagcttgc ctgccgtcta ctgcggtctt ggcgacacgc tggcctacga aggccgctac
     6121 gcccaagcca tccctcactt cgagagggcg atcaacctca gcccctacga tccccagcgc
     6181 tgggcgttct actcgtacgg ggcgctcgcg caaatcttcg cgggggactt cgacagagca
     6241 ttcgggtggg cgcagagagc cattcggatt ccaaactgtc actactggcc ctttgcccac
     6301 cgggtggctg cgcttgggca catgcagcgg gaggaggaac tgaggattgc cgtcgcagag
     6361 cttctgcggc gaaagccggg attctcgtgc ggctttgcgc gcaagcggct cttttacgtt
     6421 aagaaccctc tgcagttgaa ccggtatgtt gagggtctcc gcaaggcagg catacctgag
     6481 tgaattgaac gggcgcgaca gcattttgaa tgtcggctaa tgggatggcc cggagcaaat
     6541 tgctccactt ccgaggtctg atgcattgat gcagctctca cgttcgttga tcttggagtc
     6601 catcttgtcc gcacggccgg gccatacatt gtggcacgaa gcggcggatc gcattttgat
     6661 gctcgagggg cgctttcggc gcgaaccgga catgcatcga agtatgttcg gcatagtgcc
     6721 gaggctcagg gggaggggca gctgccagtt agatgcgtca aaaaagaact agctctcgct
     6781 cgaacttggt cgagcggggt ctattcggga cgcggcgcgt tcaaggacgc tctcgcactg
     6841 tactggcaag ctgcttacgc ccagccgcgg acatggctgg gcgatttatg agtacacgcc
     6901 ttagtgtggt tttggatttg aagttcctaa gcgagctcgc tgcacactgg tagaacttca
     6961 aatccacact ggcgcgctac ggcacgatgc cgtcgcgccc gacattgacc cgtgcggcct
     7021 cgaacgtgcc gtccggcttc ttggtcgcgg cgatgatatt gatgttggcg cccggcttga
     7081 gctcgtccct gctgccgacc acgtaggcgc ggatcacggc atcggggccg acgatg
//
LOCUS       DATLJT010001017         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1447481,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001017 DATLJT010000000
VERSION     DATLJT010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447481"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VM910_23390"
     CDS             <1..232
                     /locus_tag="VM910_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65499.1"
                     /translation="YIDTICMAGRLMGAMTTYHITIRDRETQTVLGYYNGSWTTDRCR
                     ALDLRKREVAEAHAALCASAVRATPTSSKSRN"
     gene            336..755
                     /locus_tag="VM910_23395"
                     /pseudo
     CDS             336..755
                     /locus_tag="VM910_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(898..1212)
                     /locus_tag="VM910_23400"
     CDS             complement(898..1212)
                     /locus_tag="VM910_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HVI65500.1"
                     /translation="MRATAQSRRKSVPTQTPAKKASAGKANALQKPLQPSEALAAVVG
                     SEPVPRGQVVSKIWDYIRSHKLQNPENRREILADDKLRKVFGKDKVTMFEMNKHLAQH
                     LK"
BASE COUNT          493 a          847 c          808 g          486 t
ORIGIN      
        1 ttacatcgac acaatatgca tggcggggag attgatgggc gcaatgacga cctaccacat
       61 cactatccgc gaccgcgaga cccaaaccgt cctcggttac tataacggct cctggacgac
      121 ggacagatgc cgcgccctcg atctccggaa gcgcgaggtg gcggaagcgc atgccgccct
      181 atgcgcgagt gctgtccgcg caacgccgac ctcatcaaag tcgaggaatt agacgccgcc
      241 gactgcttaa gcggcatgcg gctcggtgag gggacgcgcg agtcgtcgaa ccggaccgtt
      301 tgttttacgc tagcgggcac aagtgcgcgg ccgccccgag cgagctttat ccccgcgtcg
      361 gctttatcgt caccaacttg acgcggccag tgcagcgcgt cgtcgccttc tacaaccagc
      421 gcggcacggg ggagcaatgg atcaaggaag gcaaaggcgc aatcaagtgg acgcgactgt
      481 catgccgcac gttcgccgcc aacgccgtcc gcctccagct ccatgcgctg gcctacaacc
      541 tcggcaactt catgcggacg ctggcgatgc ccaaggcggc ggagccgtgg tcgctgacca
      601 gcctgcgcga gaagctaatc aagatcggcg ccaaggtcgt cagccatggc cgctacgtca
      661 ccttccagat ggccgaggtc gcggtgccac gacagatgtt ccaggaaatc ctgtcgctga
      721 ttgcccggtt gcgggcaccg cccgcgccag cataaggggc caacggcatc aaatgcgcca
      781 ggcgatgaca gcggaggtgt gccttgatgc aaccggggag cctggttcag cgccgcgggg
      841 tgggcaaccg gtggctttgg acgccccacg tggccgcatc acgtgttgcc gggcctctca
      901 cttcaaatgc tgagcgagat gcttgttcat ctcgaacatg gtcactttgt ccttgccaaa
      961 gaccttccgg agcttgtcgt cggccaggat ctcgcggcgg ttctccgggt tctgtagctt
     1021 atgcgaacgg atgtaatccc aaatcttgct caccacctgg ccccggggca caggctccga
     1081 acccacgacc gcggcgagcg cctccgacgg ttggagcggc ttctgcagag cattcgcctt
     1141 gcctgcgctg gcctttttcg cgggtgtttg ggtcggcacg gatttcctcc gggattgcgc
     1201 tgtggcccgc atcgcgccgc gatatctgca tcgcgtttgc ccggcacgca cgcagactat
     1261 ccgcagcgtg acgcgggtac taccggccta accctccacc cgtaccggag gttcccttaa
     1321 ggccggcgct gcccgacgaa agagcggccg ctaatcaacg aagaccatct gatcttgtcg
     1381 acgatccact ccgccaaagg gcaggaatgg gacaccgtct atattctcaa tcggcggcat
     1441 ttgagacgtc cgcagtgatg ggctccgccg ctgaggaact gatgattcga accatcggtc
     1501 ctgctcgtgt gaaaggtctc tcggctgttg ccgacgagag atgccggacc gataagttgg
     1561 atggggtcat taagcgcagg tcgtctcgcg agacgacgat ggtcggccgc ggggcctctc
     1621 tcgacggccg tctctttctc ggcggggacc gatggttcga atccggttcc ctccagcgga
     1681 gagtcgcact gcaaaccggt cccgcggggc gtcgagcgaa ttgaagcacc tcggatcagc
     1741 gggggaccaa tcggcccgct ggggatgtgc gtgctgccct gctgatctcg ccgccgtagc
     1801 cgaatcaccg gcaaagttcg aagctcgccc tctctccagc cacggacgtg aatgtccggt
     1861 tttcagcgca caccgccggc atcagcacga tgctttccgc gtcgccggtg aaggtaaggc
     1921 ggccggccgg ccggcgccag ggtgggatca cggagttttc ggcgtaccga ttccgccgta
     1981 gcagaatcgt tcacatcgaa cctaacctga tactgccact gagaagtcac gctattctcc
     2041 attttgatcg cacccgattt gcgacttgct gatcggaggg gcgctgctgc ctcctcacga
     2101 gcaggtccgc acgcaacccc atgacgggca ttgtcaactt agcggaccga gggcgccttt
     2161 ttttcgcccc gcttggcttg agagcgggca gcacactagg tgagcaaagg agttctccga
     2221 cccgagccgc cgcgccaccg acacgaagag cataccacgg cggcttaagt tgacaatgcc
     2281 accggagccg ctcgtctgaa cgacagagcg ccagcatcgg gatgccggta gtcttcgacg
     2341 cgatgccacg ggacccgcgc gcggacatac atgtcgaacg aaccgctgcc gatagcgatt
     2401 gctttattga gcgcatcgcg cgcctcgccc acgcgtccgg cctgggatcc cttgagcggt
     2461 tgcatccata cgccggctct gtcggggtaa tatccgagcc gacatgggtg ataaaatgcg
     2521 tcgtgaagtt gagatcgacg tcccgcatcg ccaccatccg acagggagcc ccgcgtcgtg
     2581 aacgaagccg atctccctcc tcctcgttac cgccaagagc agctggaggt ggcc
//
LOCUS       DATLJT010001018         4897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2136125,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001018 DATLJT010000000
VERSION     DATLJT010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136125"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VM910_23405"
     CDS             <1..215
                     /locus_tag="VM910_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65501.1"
                     /translation="KRKAGTRRSLSYRRFATAAEAIRFAVEELPAVRTLGPWMQVGEE
                     RFNSDDIERLYESTSYPRRHRMRKSR"
     gene            complement(385..1200)
                     /locus_tag="VM910_23410"
     CDS             complement(385..1200)
                     /locus_tag="VM910_23410"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65502.1"
                     /translation="MRASCSGPLWGRPSRRTYRPRHSRRGWRIPFLLGLVVGVGGYVL
                     RRQMLETTATPRRERAPIVETLHDHWRTVLGFAGLSVFNAVGFYVSFVYLVSWLQNAD
                     HIAAARALEINSFSMAILLVVVVASGLLTDRFGRKPLLLLATILGFIGAMPLFWLLDH
                     PSVWLAQLGQLGLVLIVGLYQGAQPAILVEAAPLKVRCTAVSLGYNIAFGVIGGLTPL
                     VASWLVARTGDEIAPGFLMMVAAAVTFLTLLRFSETYRTPFAGTRSRAAAAYA"
     gene            complement(1216..1668)
                     /locus_tag="VM910_23415"
     CDS             complement(1216..1668)
                     /locus_tag="VM910_23415"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65503.1"
                     /translation="MNFAQTRRVIVAGIIGNVLEWYDFAVYGFFAATIGQEFFPHQDR
                     VAQLLSAFGVFALGYLMRPLGGAITGHIGDRFGRCAALTFSVVAMAIPTFLIGLLPGY
                     GTLGLLAPIALTLLRMVQGLSVGGEYTSSMVFLVEQAPDGRRGLMGAP"
     gene            complement(1740..2321)
                     /locus_tag="VM910_23420"
     CDS             complement(1740..2321)
                     /locus_tag="VM910_23420"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI65504.1"
                     /translation="MANTHSWVVSENSDKAIEGATPLIRDRVIWLQTIFQKKSVAALP
                     VVTYQAGETVIAEGSRTGRLLILTKGVVAIVKKDTEIAQVAEPGAVFGELSVLLGKPH
                     TAEVRALETSQFHVANAATLFSQDPIAVLYVAATLAHRLDAANHALIQLKSQLKAGQP
                     NSVVAKTVSKIEGLLAVSGASLVYAGYPFDPFA"
     gene            complement(2422..2844)
                     /locus_tag="VM910_23425"
     CDS             complement(2422..2844)
                     /locus_tag="VM910_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HVI65505.1"
                     /translation="MTSEQVKAIQDSFAKVAPISEQAAEIFYGRLFEIAPAVKPLFRG
                     DMKEQGKKLMAMLTVVINGLSNLEAVLPAASALAKRHVGYGVKATDYAPVGEALLWTL
                     ERGLGEHWTPDVAAAWTAAYTVLSEFMIGEAYDHRIAA"
     gene            2999..4570
                     /locus_tag="VM910_23430"
     CDS             2999..4570
                     /locus_tag="VM910_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI65506.1"
                     /translation="MQFLFEDHVLDTDRRELRRGAQEIPVGPQVFDLLVYLVQNRERV
                     VTKDELLDAVWSRRFVSESNLTTRINAARKAIGDSGEEQRLIRTVPRKGFRFVGVVSI
                     AAETATKEVPARTATTRIPLPLPDKPSIAVLPFQNLSGDPEQEYFTDGMVEDITTALS
                     RIGWLFVIARNSSFTYKGKTVDVKQVGHELGVRYVLEGSVRKGGSRVRITGQLIDATT
                     GGHIWADRFDGTLEDVFELQDRVTASVVGAIEPQLQRAEIQRAGRKPTESLDAYDYFL
                     RGTARLHLYTKDSLLEARELYQRATELDPNYASPYGMAAWCILRLRVNGRLTDPDREI
                     ADGVRLARRAAALGKDDPTALSSSGYCLARLAGEVETGAAHIERALTLNPNLAAAWSA
                     SGWVRIFLGEPADAIKLFERAIRLSPLDLFAFIACAGMSAAHLFAGRYDEALSWVRKA
                     SHEEPNAATTWQLAAVAYALSDRIVEARKAMARLREIDPGLRLSNLARVALPFRRPEE
                     LACWTEGLRKAGLPE"
     gene            complement(4693..>4897)
                     /locus_tag="VM910_23435"
     CDS             complement(4693..>4897)
                     /locus_tag="VM910_23435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65507.1"
                     /translation="HAANKRDELAPSHVPSQAENTPYQRDPDCASQQIRVADVRFGSK
                     ADISECPSDVRFTPKSRHWNSAA"
BASE COUNT         1041 a         1441 c         1477 g          938 t
ORIGIN      
        1 caaaacgtaa ggccggcacg cgtcggtctc tcagttatcg tcgattcgcc acggcggcag
       61 aggccattcg gttcgcggtc gaagagttac ccgccgttcg cacactcggt ccatggatgc
      121 aggtgggaga ggagcgcttt aacagcgatg acatcgaacg cctctacgag agcaccagtt
      181 atccgcggcg ccatcgcatg cggaaatcga ggtgaaatct gagatttcga ctgagatagc
      241 cgtcgctgac agcgcgacct gcggcgcacg cgatcgataa tatcgatatg ccgctacttg
      301 acccgaagat tgatcgcgct cagcggtcct gcgcgactga tgagaaaagt gaatttcgtg
      361 ggcactgacg agccccacgc accgctatgc atatgccgca gcagccctcg acctggtgcc
      421 agcaaacggg gtgcggtagg tctctgaaaa tcgcaggagg gtcaagaatg tgacggccgc
      481 cgccaccatc atcaagaatc caggagcaat ctcatccccg gtgcgtgcga ctagccaaga
      541 tgcgacaagc ggcgtcagcc caccgatcac cccgaaagct atgttgtaac ccaaagaaac
      601 cgcggtgcag cgcaccttca ggggcgccgc ttccaccaaa atggcgggtt gtgcgccttg
      661 atagagcccg actatcagca ccagaccgag ttggcctagc tgcgcaagcc atactgacgg
      721 atgatctaaa agccagaaca gcggcatcgc gccgatgaaa cccagtattg tggcgaggag
      781 gagcagcggt ttgcgtccga accggtctgt caacaagccc gacgccacta ccaccaccag
      841 gagtatcgcc atgctgaaag agttgatttc gagcgcgcgt gcagccgcaa tgtgatcagc
      901 gttttgcagc caactcacca gatatacgaa gctgacatag aagcccactg cattgaatac
      961 tgacaaacca gcaaatccga ggacggtgcg ccagtgatca tggagtgtct ccacgatcgg
     1021 ggcgcgttcg cggcgcggcg tcgcggtggt ctccagcatc tgccggcgta gcacgtatcc
     1081 tcctacccca acgaccagcc cgagcaggaa cggaatgcgc cagccccgcc tggagtgccg
     1141 cggccgatag gttcgccgtg aaggccgccc ccacagagga cccgagcagg atgcccgcaa
     1201 tggcgccgca cgccgtcagg gcgcgcccat caacccgcgc cggccatcgg gtgcctgctc
     1261 gaccaggaac accatggagc tcgtatattc accgccaacc gaaaggccct gcaccatgcg
     1321 cagaagcgtg agcgcgatcg gcgccagcaa accgagcgtc ccgtatccag gcaataaccc
     1381 aatcaggaac gtgggaattg ccattgcgac aaccgagaag gtgagcgccg cgcaccgacc
     1441 aaaacggtcg ccgatatggc cggtgattgc accgccgaga ggtcgcatca aatagccgag
     1501 tgcaaatact ccgaatgcgg aaaggagctg ggcgacccga tcctgatggg ggaagaactc
     1561 ttggccgata gtcgcggcaa aaaagccgta gacagcgaaa tcgtaccatt cgagcacgtt
     1621 gccgattatg cccgcgacga tcaccctacg cgtttgagca aaattcatat tcacatactc
     1681 cctgagccga cctccaagcg cgtggaagtg tcgtgttctc gcgtcgttta tctcgcttgc
     1741 tacgcaaagg gatcgaatgg atatccagcg taaacaagac tagcgccgct gacggcgaga
     1801 agtccttcta ttttactgac cgttttggcc accacgctgt ttggctggcc cgccttaagt
     1861 tggctcttga gttgaataag ggcgtgattg gcggcatcga gtcggtgtgc caacgttgca
     1921 gcaacataaa ggaccgcgat cggatcttgc gagaaaaggg tggccgcgtt ggctacatga
     1981 aactgcgagg tttccaaggc acgcacctcc gccgtgtgcg gcttgcccag caagacggag
     2041 agctcgccaa aaacggcacc aggctccgcc acctgagcaa tctctgtatc cttctttacg
     2101 atcgcgacta cgcccttcgt gaggatgagc agccgaccgg tccttgaacc ctcggcaatc
     2161 accgtttcgc cggcctgata ggtcacgacc ggaagcgcgg caacgctctt tttttgaaag
     2221 atagtttgaa gccagatgac tctgtcacga atgagcggcg tggcaccttc aatcgctttg
     2281 tcgctgttct cgcttactac ccacgaatga gtgttagcca cttggaatgg acgcgctggg
     2341 tctggagagt gcaacatgac aaaacctcat ggggaccagt gggtgcccgc agctgaatgt
     2401 ctgactgggc aacaagccgg ttcaggccgc gatcctgtgg tcgtaggctt cgccgatcat
     2461 gaattccgag agtacggtgt aggcagcagt ccaggcggcg gcgacatccg gcgtccaatg
     2521 ctcgcccaag ccgcgctcaa gcgtccacaa caacgcctcg cctaccggtg catagtcggt
     2581 cgccttgacg ccgtatccga cgtggcgttt ggcgagcgcg cttgccgccg gcaacaccgc
     2641 ttctagattg ctgagcccgt tgatcacgac cgtgagcatc gccatcaact ttttgccctg
     2701 ctctttcatg tcaccgcgaa acagcggttt aaccgcaggc gcgatctcaa ataggcgacc
     2761 gtagaaaatt tcggcggcct gctcagatat cggtgcgact ttggcgaaac tatcctgaat
     2821 cgctttcacc tgctcagatg tcatccgcat ttgaattctc cactttgtgt cgcgagcatc
     2881 gcgataacgg gaatgatgcc tgtgcgggcg gggaaagact ttaaacggcg cttaattggc
     2941 ccttaaaatc tgcctgattc ttgcttaaaa ttgccgatgc gaccccggga gggtcgaagt
     3001 gcaatttctc tttgaggacc acgtcctgga taccgaccgg cgcgagcttc ggcgaggcgc
     3061 ccaggagatc cccgtcgggc cgcaggtttt cgatttgctg gtttatctgg ttcagaatcg
     3121 agagcgtgtg gtcaccaagg atgaactgct agacgcagtt tggtccagac gtttcgtttc
     3181 ggagtcgaac ttgactaccc gcatcaacgc cgcgcgcaaa gcgatcggcg acagcggtga
     3241 agagcagcgg ttgattcgca cagttccacg caagggcttt cgattcgtcg gcgttgtgag
     3301 tatcgcggcc gagacagcga caaaggaggt gcctgcccgc accgctacga cgcgaatacc
     3361 gttgcccctc ccggacaaac cgtcgattgc cgtgctgccg ttccagaacc tgagcggcga
     3421 tccagagcag gagtacttca ccgacggcat ggtcgaggac atcacaacgg cgctgtcacg
     3481 catcggctgg ctgttcgtga tcgcccgaaa ctcgtctttc acctacaagg gcaagacggt
     3541 cgacgtgaag caggttgggc acgagctggg tgtgcgctat gtgctcgaag gtagcgtgcg
     3601 caagggtgga tctcgggtgc gcattacagg acagctcatc gatgcgacga ccggcggcca
     3661 catttgggcg gatcgcttcg acggcactct cgaagacgtg ttcgagctgc aggatcgcgt
     3721 gaccgcaagt gtcgttgggg ccatcgagcc gcagctccag cgggcggaga tccagcgcgc
     3781 gggacgaaag ccgactgaga gcctggacgc ttacgactac ttcttgcgcg ggacggcccg
     3841 cttgcatctc tacacaaagg acagcctctt ggaagcaagg gagctttacc agcgcgcgac
     3901 agaactcgat cccaactatg cctcgccata cggtatggca gcctggtgca ttctgcgatt
     3961 gagggtgaac ggccggttga ctgaccccga ccgcgagatc gcggacggcg tgaggctggc
     4021 gcgtcgcgcg gcggctctag gcaaggatga tccgacggcg ctgtcgtcga gtggctattg
     4081 cctcgcccgt ctcgccggtg aggttgagac aggggcggcc cacatcgagc gcgcactcac
     4141 tctgaacccg aacttggcgg cagcctggag cgcgagcggg tgggtgcgca tctttcttgg
     4201 cgaaccagcc gacgcgatta aactcttcga gcgagccatc cggctgagcc cgctcgatct
     4261 gttcgccttc atcgcctgcg ccggtatgag tgctgcacac ctcttcgcag gtcgttacga
     4321 cgaggcacta tcttgggttc gaaaggcgag ccacgaggag ccgaatgcgg cgacgacgtg
     4381 gcagttggca gcggttgcct atgccctatc ggataggatc gtagaggctc ggaaagcaat
     4441 ggcccgactg cgcgagatcg atcccggtct gcgcctaagt aatcttgcaa gagtagcgct
     4501 cccgtttcgt cggccggaag agctcgcctg ctggaccgag ggcctgcgca aggccgggtt
     4561 gccggaataa tcgcggcgtt ctgcgcggcg agcatcggcg attgggccat tcggaggagc
     4621 ccgcgccgac tgcaatacga acggcgtcaa attgctgcat cgcgtatgtc tgcttttggc
     4681 acaaagcgga catcacgccg ctgagttcca atgtctgctt ttgggggtaa agcggacatc
     4741 gctcgggcac tccgaaatgt ccgcttttga cccaaagcgg acatcggcga ctctaatttg
     4801 ctgtgatgca caatccgggt cgcgctggta cggtgtgttc tcggcttgtg agggcacatg
     4861 agacggcgcg agttcatcac gcttgttggc ggcgtgg
//
LOCUS       DATLJT010001019         1672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4434766,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001019 DATLJT010000000
VERSION     DATLJT010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4434766"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VM910_23440"
     CDS             complement(<1..158)
                     /locus_tag="VM910_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HVI65508.1"
                     /translation="MRPRERRETSEQELFRSRLDQIIDMKHALVKLGQAIDWRFLEER
                     FGAVYTDKP"
     gene            582..1022
                     /locus_tag="VM910_23445"
     CDS             582..1022
                     /locus_tag="VM910_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65509.1"
                     /translation="MIWIENTRSVKGIQPRDQVEAMLAAQMAAVHRATMAFAQRLELA
                     RPYHSRTAWSAPSTSLARTFESQMQALKRYRTGGEQKLTVQNVSVNDGGQAIVGNVTQ
                     APREAVRGGAAASPAALVDAQCPPMAVLNEPAPAPIPFKRKAGR"
     gene            1052..1288
                     /locus_tag="VM910_23450"
     CDS             1052..1288
                     /locus_tag="VM910_23450"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HVI65510.1"
                     /translation="MLASLRCGAKTRSGKACRSPAVQGKKRCRMHGGAAGSGAPRGNQ
                     NAVTHGLYRKAAIEERRQVGTLIRQSRKLLQDIE"
     gene            complement(1291..>1672)
                     /locus_tag="VM910_23455"
     CDS             complement(1291..>1672)
                     /locus_tag="VM910_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVI65511.1"
                     /translation="SVFGTDYPTPDGTCIRDYIHVSDLIAAHRNALAHLRAGGPSATL
                     NCGYGRGFSVLEVIESVKRVSGVDFKVEFTGRRAGDPAQLVAACDRVRAMLNWEPRFD
                     DLATIVSHALDWERRLQRVPVVSA"
BASE COUNT          368 a          485 c          522 g          297 t
ORIGIN      
        1 ggcttgtcgg tgtacaccgc cccgaacctc tcctccagaa aacgccaatc gatcgcctgc
       61 cccagcttca ccaacgcgtg tttcatgtcg atgatttggt caagccgtga gcggaacagc
      121 tcctgctcac tggtctcccg gcgctcgcgt ggtcgcatcg caaatcctcg ctccatcggc
      181 tcgagcgaga agaatcacag cttgcgattc gagggaatcc tcaaaatcga tttgcaagga
      241 aatcgcactc caatggtcaa aaaccggcaa attcgattac ttgagcgggc ccaatcatcc
      301 agcccattca atagctttag aattcttcac gggcgactct ctagtgcggg aatgcccgca
      361 aaacgaacgg ctcggcgtcc ttggtctgcg gtttcgggag cggccgatga tccgcggatc
      421 ctcggtggtc gacccaattc cttcggaaag ctcggtataa acgttctttc cgagatactt
      481 gccctcaaac gaccataaca attttcgagc gcgtgacatc tcccctagaa gcgctactta
      541 ggtggtatga aagcgcgaat gaccgatgcc aagaagactt tgtgatttgg atcgaaaaca
      601 ccagatccgt caaaggcatt cagccgaggg atcaggtgga ggcgatgctg gcggcgcaga
      661 tggccgctgt acatcgggcg acgatggcat tcgctcagcg gcttgagctc gcaagaccat
      721 atcacagcag gacagcgtgg agcgcgcctt caacaagtct cgcccggacg tttgaaagcc
      781 agatgcaggc gctcaagcgc taccgcacgg gtggcgagca gaagctcacg gtgcaaaatg
      841 tgtcggtcaa cgatggtggg caggcgatcg tgggcaatgt gactcaggcg ccacgcgagg
      901 ccgtgcgagg cggggctgcg gcgtcaccgg ccgcactcgt tgacgctcag tgcccaccga
      961 tggcggtgct aaatgaaccg gcgccggcgc caattccatt caagcgcaag gccggcagat
     1021 gaccggcaac catctgcgca atactggccc gatgctggcc agcttgcgct gcggcgccaa
     1081 gacccgctcg ggcaaggctt gcaggtcgcc ggcggtgcag gggaaaaagc gctgtcggat
     1141 gcatggcggt gcggcgggat caggcgcacc gagaggcaat cagaacgcgg tcacgcacgg
     1201 cctttatcgg aaagccgcga ttgaagagcg gaggcaggtt gggacgctca tacggcagtc
     1261 gcggaaactg ctgcaagaca tcgagtgacg ctatgcggaa acaactggca cacgttgtag
     1321 cctgcgctcc caatccaatg catggctgac aatggttgct aaatcgtcaa aacgcggttc
     1381 ccagttgagc atggcgcgaa cgcgatcgca ggcggcgacg agctgggcgg gatctccggc
     1441 gcggcggccg gtgaattcga ccttgaaatc gacgccggag acccgcttca cgctctcgat
     1501 cacctccagt acggaaaagc cacggccgta gccgcaattg agcgtggcgg aagggccgcc
     1561 ggcgcgcaga tgggcgagcg cgttgcggtg cgcggcaata agatcggaca cgtggatgta
     1621 atcgcggatg caggttccgt ccggcgtcgg atagtcggtg ccgaagacgc tc
//
LOCUS       DATLJT010001020         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2826751,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001020 DATLJT010000000
VERSION     DATLJT010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826751"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VM910_23460"
     CDS             complement(<1..205)
                     /locus_tag="VM910_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HVI65512.1"
                     /translation="MARLLARRPDGIFVAPFEQGDIGPDLFRKACKMGLEGMVSKRRE
                     RAYRAGRCNHWIKVKPAIRPCDES"
     gene            647..1057
                     /locus_tag="VM910_23465"
     CDS             647..1057
                     /locus_tag="VM910_23465"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI65513.1"
                     /translation="MEVITGQRRRRHWTAEEKARIVAESFEEGANISQVARRHGVVRG
                     LLTVWRRKFATAAMFQAPGFVPVRIASESGPARADEADGLACAHKVLPQMSSPPGKLG
                     GMIEIEVEGACIRVEPGVELATLSTVLSALRGIR"
     gene            1054..1407
                     /gene="tnpB"
                     /locus_tag="VM910_23470"
     CDS             1054..1407
                     /gene="tnpB"
                     /locus_tag="VM910_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970376.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HVI65514.1"
                     /translation="MIALRSDLKVVLAAQPVDFRKSVHTLSALVSEALRANPYCGDVF
                     VFRSKRADRVKLLAWDGSGMVLVTKWLHQGHFTWPPIRDGVVHLSATQLAMLLDGLEW
                     TRVSPKPVKQPAVVG"
     gene            1458..3017
                     /locus_tag="VM910_23475"
     CDS             1458..3017
                     /locus_tag="VM910_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI65515.1"
                     /translation="MAIRPEALPTDAAALTEMVLALDAENEKLRVAMQTLKEMIFGKR
                     SERLAAIVAEQLALELDDLATGVTPPAPANDDLPATKPAGKPRKKARRNIGALPKHLP
                     RCEQVLEPDTAACPCCQGLLHRIGEDVSEVLDVIPAILRVLRTIRPKYACRGCTDGVV
                     QAKVPPRLIESGMASTALVAHVVVSKFAWYLPLYRQVQILAGQGLHLDRATLAGWVKR
                     AAWWLKSLYELQLRTIQASPRLFCDETPMPVLDPGRHRTRICQFWAHAMDDRPWGGPS
                     PPAVAYVFADGRGTEEIAGQLTGFSGILQVDGYAAYKALARGQGGAIQLAFCLAHARR
                     KFVEVFKTMQSPFAHEVIERLQAVYAIEAEIRGSSAEQRLAARRTRTAPLMEVLKARL
                     TSMLDQLFSQSKLAEAINYALNHWDGLTLFLRDGRVEVDSNTVERSMRPIAMGRRNSL
                     FSGSEGGAESWAILASLVNTAKLHELDPQAYLADVLERIVSGQTKSHQLHELLAWNWK
                     AARGLSAWVAA"
     gene            3014..3640
                     /locus_tag="VM910_23480"
     CDS             3014..3640
                     /locus_tag="VM910_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HVI65516.1"
                     /translation="MSRRRRSSSSSSMAAAAMPLDELEAWLQARAKQHPIATSLPVLD
                     GYVAAIVAGPVSMSPPDWICPLLAIDADAFNHGGTPEFAAISAVALRHNDISNVLSTA
                     PDRFAPVHGRKPNGDVDARPWCQGFHAAMRLQLSAWAPLLDVSNIHHGLLLPILLHCV
                     DDQGRPLLGPPRKGRETEEFLRNAHADIPDVVEAMRQYWMPTRYARTR"
     gene            3643..3839
                     /locus_tag="VM910_23485"
                     /pseudo
     CDS             3643..3839
                     /locus_tag="VM910_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          681 a         1160 c         1293 g          705 t
ORIGIN      
        1 ttgactcgtc gcatggccgg atggctggct tgaccttgat ccagtgattg caccggccgg
       61 cgcggtaagc gcgttcgcgg cgcttggaca ccatgccctc gagtcccatc ttgcaggcct
      121 tgcggaagag gtcgggaccg atgtcgcctt gctcaaaggg cgcaacaaag atgccgtccg
      181 gccggcgcgc gagtaaccgc gccaagttgg tctttcgcag ggacgacctt gccaccgggt
      241 cgggaggcgt ggctggaacg tgcttcgcat gccgccgaga tagggattct tgatgaaagt
      301 cgcgaatcta atcgaacaca aaacggcccc gagcaacggg gggtgccggg gccgcccagc
      361 ctctcggcgg gggagactgg ttctcccggc ctcgggctaa tgcgggtcga ggtcgggagc
      421 atcgaatctg agcccgccgc ccgccgcttg ttcccttgag gcgtcagccg tccagtctcc
      481 agctcatgcg cgatatagcg taaccggcac gagcccccca cctgggcatc gcgcgaatga
      541 gctgcgatac agcctggcat gaactgtttt ttggaggtgg gctatggcag atgccatgca
      601 tgaagccatg cttgatgcca ggcaggaaag cggctcctat cgtcggatcg aggtgatcac
      661 ggggcagcgt cgacgacggc actggacggc ggaagagaag gctcggatcg tggccgagag
      721 tttcgaggaa ggggccaata tttcccaggt cgctcggcgc catggcgttg tccgtgggct
      781 gctgacggtg tggcggcgca agttcgcgac ggcagcgatg tttcaggcgc caggctttgt
      841 gccggtccgg atcgcttccg agagcggtcc ggcgagggca gacgaggcgg acgggttagc
      901 atgcgcgcat aaggtgctgc cacagatgtc atcgccccca ggcaagcttg gcgggatgat
      961 cgagatcgag gtagaagggg catgcatccg ggtcgagcca ggagtggagc tggcgacgct
     1021 ttcgacggtg ctatcggcgc ttcggggcat ccgttgattg cattacggtc tgacctcaag
     1081 gtggtgctgg cggcacagcc ggtcgacttt cgcaagtcag tgcacacgtt gtcggcgctg
     1141 gtgagcgaag cgctgcgtgc gaacccgtat tgcggcgacg tcttcgtgtt tcgcagcaag
     1201 cgcgcggaca gagtgaagct tttggcgtgg gatggcagcg gcatggtgct ggtgacgaag
     1261 tggctgcacc agggacattt cacctggccg ccgatccgcg atggcgtggt gcatctaagc
     1321 gcgacacaac tcgcgatgct gctcgacggg cttgagtgga cgcgtgtttc gcccaagcct
     1381 gtgaagcagc cggccgttgt cggctgagag gcgaagattt tgctggagcc tatgatgcgc
     1441 ggatatatcg ttcgatcatg gcgattcgtc ccgaagctct tccgaccgat gcagcagctc
     1501 tgaccgagat ggtgctcgcg cttgacgccg agaacgagaa gctacgtgtg gcgatgcaga
     1561 cgctgaagga gatgatcttc ggcaagcgct cggagcggct cgcggcgatc gtggccgagc
     1621 agctcgcgct cgaactggac gatcttgcga ctggcgtcac accacctgcg cccgccaacg
     1681 acgacttgcc tgcgacgaag ccggctggga agccgcgcaa gaaagcgagg cgcaacatcg
     1741 gcgcgttgcc caagcatctg ccgcgctgcg agcaggtgct cgagccggac acggcagcgt
     1801 gcccgtgctg ccagggcctt ctgcacagga tcggcgagga cgtcagcgag gtgctggacg
     1861 tgatcccggc gatcctgcgg gtgctgcgca cgattcgtcc caaatacgcg tgccgcggct
     1921 gcaccgacgg cgtggtgcaa gcgaaggtgc cgccgcgtct gatcgagagc ggaatggcat
     1981 cgacggcact cgtggcccac gtggtggtct cgaagttcgc ctggtatctg ccgctgtacc
     2041 ggcaggtgca gatcctggcc ggtcagggcc ttcatctcga ccgcgcgacg ctcgctggct
     2101 gggtgaagcg tgcggcatgg tggctcaaga gcctttacga gcttcagctg cggacgatcc
     2161 aggcttcgcc gcggctgttc tgtgacgaga cgccgatgcc ggtgctcgat cctggacgac
     2221 atcgcactcg tatctgccag ttctgggcgc atgcgatgga tgatcgcccg tggggcggcc
     2281 catcgccgcc ggcggtcgcc tatgtgtttg ccgatggccg cggcaccgag gagatcgccg
     2341 ggcaattgac cggcttctcc ggcattctgc aggtggatgg ctatgccgcc tacaaagcgc
     2401 ttgcccgcgg tcaaggcggg gcgatccagc tcgctttttg tctcgcgcat gcccgacgca
     2461 aattcgtcga ggtgttcaag acgatgcagt cgccgttcgc tcacgaagtg atcgagcgcc
     2521 tgcaagcggt ctatgccatc gaggccgaga tccgcggctc gagcgccgaa cagcggcttg
     2581 ccgcccgccg caccaggacc gcaccgctga tggaggtgct caaggcgcga ctgacctcga
     2641 tgctcgatca gctgttctcc cagtcgaagc tggcggaggc catcaactat gcgctcaatc
     2701 actgggacgg actgacgctg tttctccgcg acggtcgcgt cgaggtcgac agcaacaccg
     2761 tcgagcgttc aatgcgcccg attgcgatgg gaaggcggaa ctcattgttc agcggcagcg
     2821 agggcggtgc cgagagctgg gcaatccttg cgtcgctggt caatacggca aagctccacg
     2881 aactcgatcc gcaggcgtat ctggccgatg tgctggagcg catcgtctcc ggtcagacca
     2941 agagccacca gctgcacgaa cttctcgcct ggaactggaa ggcggcccgc gggctcagcg
     3001 catgggtggc cgcatgagcc gccgccgccg ttcatcatct tcatcatcga tggcggcagc
     3061 cgcgatgccg ctcgatgaac ttgaggcctg gctgcaggct cgcgccaagc agcatcccat
     3121 cgccaccagt cttcccgtgc tcgacggcta tgtcgccgcg atcgtggccg ggccggtgtc
     3181 gatgagtccg cccgactgga tctgtccact actcgccatc gacgccgatg cgttcaacca
     3241 tggcggcacg ccggagttcg ccgcgatttc ggccgtcgca ctgcgccaca acgacatcag
     3301 taatgttctt tccaccgcgc cagatcggtt cgcgccggtc catggccgca agccgaatgg
     3361 cgacgtggat gcgcggccgt ggtgccaggg attccatgcc gccatgcggt tgcaactatc
     3421 cgcctgggcc ccactgctcg acgtcagcaa catacaccac ggcttgctcc tgcccatcct
     3481 gctgcattgc gtcgacgatc aagggcgtcc actgctcgga ccaccaagga aaggccgcga
     3541 gactgaggaa ttcctgcgca atgcccatgc cgacattccg gacgtcgtcg aggccatgcg
     3601 tcagtattgg atgccgaccc gctacgctcg cacacgctga tcactgcgga cgtgggagct
     3661 cataccggtt actatcaatc aggcgagatc gattggagcg gtaggatatc gcgctgcgga
     3721 gatgagatga tgcgggtgat gctctacgaa gcagcgcaga gcatgttgcg ttcgaagaaa
     3781 tggtcctggc tcaaggcctg ggctatgcag atcgccaggc gccgcggaat gaagaaggc
//
LOCUS       DATLJT010001021         5383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3745663,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001021 DATLJT010000000
VERSION     DATLJT010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3745663"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3022)
                     /locus_tag="VM910_23490"
     CDS             complement(<1..3022)
                     /locus_tag="VM910_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVI65517.1"
                     /translation="MNARQLQLQGIGDPRLAVHAASALAAWLWSTDGAQVLWANPVGA
                     SLFGASNMAELARKTFGPADARRRQVVQLAGQLSPTGAIRLERLRGFGASTGMLMTCS
                     CARLDFPDGSHGILIAATEASQRIMPLTERLRLLVQGFEAPIAAFARDGMFIAACEAA
                     RPLFGFRDLAGEDFDEARNEALRHGRAEMRIGIGRIVLQRVGSGNEVGIVALIATDGP
                     HGTEPPKEDAAAPPARSEPVESTHPDAPAETPVNISTSSEASPPMAPSANDPVSRSAA
                     APSSHDEAAITPPRHPLRFTWQMDVESRFALGSDEFTRLIGTRTAAGFGRPWQEIADS
                     FGIDPAGRVVEAIATRDTWSGITVYWPVDGGGRVPVELSGFPIYDHERSFAGYRGFGV
                     CRDLDSLAHLADLRDLELFGDPAAATPAPAADIFPVDTQQEDAELVPATLADTAVTPS
                     QRHGPIADEASPQADPGEPVETPANVVMFRPAGDTKSPVLTPVENSAFDELARQLAAR
                     LLRNNAGAPESAVTSETAEMMAEPSVPQEAVAEHSAPRQVEWLQPSGPPVRGASRRDR
                     KLLDLLPTGVMIYRLDRLLYANPAFLERLGYDSLNALEQAGGLDALYVEAGVSSASST
                     SEAGTPVTISAGQDGSGPSTPTAARLFTISWDGEPAHALMFAPLPEAASASAATPTRV
                     PLPAPAPEPVAAAPLQAAGYASAEDLGAILDTTAEGIVMFDAEGDIHACNRSAEALFG
                     YDGDTLVRRNLADLFAPESQRAVAEYLDSVKNADVASLLDHGRDVLGRVSMGGIIPLS
                     MTMGRTRPGGRNFFAVFRDLSQGKKGESELQQARRLVDRAANAKADMVARISHEVRAP
                     LSAIIGFAEVMIAERFGSLGNQRYVEYLKDIRASSERVTGILDDLAELARIETGKLDL
                     AFANQNLNELVESCVTVMQPQANRERIIIRTSLAQALPPVVADGRALRQITLNLIGNS
                     IHLANPAGQVIVSTALSDFGEVVLRVRDTGHGLDD"
     gene            3379..3762
                     /locus_tag="VM910_23495"
     CDS             3379..3762
                     /locus_tag="VM910_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI65518.1"
                     /translation="MSDEARDRFEMPKEMRSMAEASVEQARKTFEKFLESAQATAGSI
                     EERGATVRAGAKDVSAKAFANAEKNVQASLDYAQSLLHAKDLSDVMRLHSEFVQAQMR
                     ALTEQASEMGQIVGRAAMEAAKPKV"
     gene            4010..4444
                     /locus_tag="VM910_23500"
     CDS             4010..4444
                     /locus_tag="VM910_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI65519.1"
                     /translation="MPMSTDPFSASVIPFEVPESMRAFAEKGVSQARDSYAKFKDAAE
                     SQNSTIEAVFTSASKGASAYSAKMMEFMKANSTSNLDFAQELLGVKSPSEALELYTGH
                     VRKQFETFTAQAKELAEIVQKMAAETSEPIKASASKFYKPAA"
     gene            4735..4811
                     /locus_tag="VM910_23505"
     tRNA            4735..4811
                     /locus_tag="VM910_23505"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4769..4771,aa:His,seq:gtg)
     gene            complement(5059..>5383)
                     /locus_tag="VM910_23510"
     CDS             complement(5059..>5383)
                     /locus_tag="VM910_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HVI65520.1"
                     /translation="ASGHPIDRDLKNRFGSRNYAVEELIAELRAAFLCAEFGFDGDFQ
                     HAGYIGHWIELLKADKRAFFTACSQASKAADYLRGLALAARTEVGARAQIGGISRSPS
                     SMSIL"
BASE COUNT          976 a         1727 c         1724 g          956 t
ORIGIN      
        1 tgtcgtccag cccgtggccg gtgtcgcgta ccctgagcac cacctcgcca aaatccgaca
       61 gcgccgtcga gacgatcact tgccccgcgg gattggcgag gtggatcgag ttgccgatca
      121 ggttcagcgt gatctggcgc agtgcgcgcc cgtccgccac caccggcggc agcgcttgcg
      181 ccagcgaggt acggatgatg atccgctcgc ggttggcctg cggctgcatc accgtgacgc
      241 agctttccac gagttcgttg agattctggt tggcgaaggc gaggtcgagc ttgccggtct
      301 cgatccgggc tagctcggcg aggtcgtcga ggatgccggt cacgcgctcg ctggaggcgc
      361 ggatgtcctt caaatattcg acatagcgct gattgccgag cgaaccgaac cgctcggcga
      421 tcatcacctc ggcaaagccg atgatggcgc tcagcggcgc gcggacctcg tggctgatcc
      481 gcgcgaccat gtccgccttc gcattggccg cccggtcgac cagccggcgg gcctgctgca
      541 gctcgctttc acccttcttg ccctgcgaaa ggtcgcggaa caccgcaaag aaattccgac
      601 cgcccggtcg ggtgcgtccc atcgtcatcg acagcgggat gatgccgccc atgctgacgc
      661 ggccgagcac gtcgcggccg tgatcgagca ggcttgccac gtctgcgttc ttcacgctgt
      721 ccagatattc ggcgaccgcg cgctggctct ccggcgcgaa cagatcggcg agattgcgtc
      781 gcaccagggt gtcgccgtca tagccgaaca gcgcctctgc actgcgattg cacgcatgga
      841 tgtcgccctc cgcatcgaac atcacgatgc cctcggcggt ggtgtcgaga atggcgccga
      901 gatcctcggc actggcgtat cccgcggcct gcagtggggc ggcggcaacc ggctccggtg
      961 cgggggccgg caggggaacg cgagtcgggg tcgccgcgga cgcgcttgcc gcctccggta
     1021 acggtgcgaa catcaaggca tgcgcgggct cgccgtccca tgaaatcgtg aacagccgcg
     1081 ctgcggtcgg cgtcgagggt cccgatccat cctgaccagc cgagatcgtc accggcgtgc
     1141 cggcttccga tgtgctgctc gccgaagaga cgcctgcctc gacatagagc gcatccagtc
     1201 cgcccgcctg ctccagcgca ttcaggctgt cgtagccgag ccgttccaga aatgccgggt
     1261 tggcgtagag cagccggtcg agccgataga tcatgacgcc ggtcggcaac agatcgagca
     1321 gcttgcggtc gcggcggctc gcgccgcgca ccggcggccc actcggctgc agccattcga
     1381 cctgcctggg cgcggagtgt tccgcgaccg cctcttgcgg tacagacggc tcggccatca
     1441 tctccgcggt ttcgctcgtc acggccgatt ctggcgcgcc ggcattgttg cgaagcagcc
     1501 gggccgcaag ctgccgggca agctcatcga aggcgctgtt ttccaccggc gtgaggaccg
     1561 gcgatttcgt gtcgcccgcg gggcggaaca tcacgacgtt tgcgggcgtc tccacgggct
     1621 cacccggatc ggcttgcggc gaagcctcat cggcaatcgg cccgtgccgt tgagatggcg
     1681 tgactgccgt gtcggctaac gtcgcgggca caagctcagc gtcctcttgc tgcgtgtcga
     1741 ccggaaaaat gtccgccgca ggggccggcg tcgccgccgc cggatcgccg aagagctcaa
     1801 ggtctcgcag gtcagccaga tgcgccaggc tgtcgagatc gcggcacacg ccaaaaccgc
     1861 gatagccggc aaagctccgc tcgtggtcgt agatcggaaa tccggacaat tcgaccggca
     1921 cgcggccgcc gccgtcgact ggccagtaca cggtgatgcc gctccaggtg tcgcgtgttg
     1981 cgatcgcttc gacaacgcgg ccggccggat cgatcccgaa cgagtcggcg atctcctgcc
     2041 atggccggcc gaaaccggcc gcggtgcgtg tgccgatcag tcgggtgaac tcgtccgaac
     2101 caagcgcgaa gcggctctcg acatccatct gccaggtgaa gcgcagcgga tgacgcggcg
     2161 gcgtgatggc cgcctcgtcg tgcgaggaag gcgcggccgc cgatcgcgag accggatcat
     2221 tcgcggaagg agccatcggc ggcgaggctt cggagctcgt gctgatgttg acgggcgttt
     2281 cggccggcgc gtctggatgc gtcgattcga ccggctcgga gcgcgccggc ggggcggcgg
     2341 cgtcctcttt tggcggctcg gtcccgtgcg gcccgtccgt cgcgatcagg gcgacgatcc
     2401 cgacctcatt gccgctgccg acacgctgca gcactatccg cccgatgccg atccgcatct
     2461 ccgcccggcc gtgccgaagc gcttcattgc gcgcctcgtc gaagtcttcg ccggcgagat
     2521 cgcgaaagcc gaacagggga cgcgcggctt cgcacgcggc gatgaacatg ccgtcgcgcg
     2581 caaaagccgc gatcggcgcc tcaaatccct ggacaagaag ccgcagccgt tcggtgagcg
     2641 gcatgatccg ctgcgaagcc tcggtggctg cgatcagaat gccgtggctg ccgtccggaa
     2701 agtcgaggcg tgcgcagcta cacgtcatca gcatgcccgt cgacgcgccg aaaccgcgca
     2761 ggcgttcgag ccgaatcgcg cccgtcggcg acaactgccc tgcgagctgc acgacctgcc
     2821 gccggcgtgc atcggcagga ccgaaggtct ttctcgccag ttccgccata ttggatgcgc
     2881 cgaacaaact ggcgccgacc gggttggccc acagcacctg tgctccgtcg gtcgaccaca
     2941 gccacgcagc aagcgcgctc gctgcgtgta ccgcgagccg tggatcgccg atcccctgca
     3001 gttggagttg tctcgcgttc atggtggcga ttggatcggc tcgtcgggtt tcgatgcagg
     3061 gttcgaggaa cgcgcgggcc gcacgatgac agccttgacg gaggattagt atcgcccggg
     3121 cggaccggca ggtccaccgc tcgccgccgg cactgggggt aaagtgggcc aaccgtaatt
     3181 ctccgggcgt tccatcaaag cgcgtcgaac cctgatgcgg cctttgcgtt gaggggtgag
     3241 gggcgatcaa aggtccgcga tgggcgatgc tcaccctgcg tcacaccagc gaagcgtttt
     3301 tcggccaggg agccgcagac cgccgtgggc aaaggggaca gcggggcgca cgcctcgcag
     3361 cgcaacagga gtggtaccat gagcgatgaa gcgcgtgacc gtttcgaaat gccaaaggaa
     3421 atgcgatcga tggcggaggc gagcgtcgaa caggcgcgca agaccttcga aaaattcctt
     3481 gagagcgccc aggcgaccgc cggctcgatc gaggagcgcg gcgcgaccgt ccgcgccggc
     3541 gccaaggacg tcagcgcgaa agcattcgcc aatgccgaaa agaacgtgca ggcctctctc
     3601 gattatgccc agtcgctgct gcacgccaag gatctgtcgg atgtgatgcg gctgcatagc
     3661 gaattcgtgc aggcacagat gcgcgcactc accgagcagg cgagcgaaat gggccagatt
     3721 gtcggtcgcg ccgccatgga ggcggcgaag ccgaaggttt gaacagcctc ggactcgcaa
     3781 tcgatggaat tatcaaaccg aggttcgaga ttggaaccac ggcgggatag gtttggcatg
     3841 tcgcattgca cctaaaattt cattgcaatg cacaaaatag acattatatt cccctacacg
     3901 tctgcaggtc ccgataaggg catccccact gcgtgatcca gttcgatgta cccggtagcc
     3961 tggctgccgg gcgcaataag actcaatctc accccgttgc gaaggatacg tgccgatgtc
     4021 taccgacccg ttctctgcct ccgtcattcc attcgaagtc cccgagtcca tgcgtgcgtt
     4081 cgccgaaaag ggcgtttcgc aagcccgcga cagctacgcc aagttcaagg acgcagccga
     4141 aagccagaac tcgaccatcg aggcggtctt cacctctgcc agcaagggcg caagcgccta
     4201 ttccgccaag atgatggagt tcatgaaggc caacagcaca tccaacctgg actttgccca
     4261 ggagctgctt ggcgtgaaat cgccgtctga agcgctcgag ctttacaccg gccatgttcg
     4321 caagcagttc gagaccttca ccgcgcaagc caaggagctc gccgagatcg tccagaagat
     4381 ggccgccgag acctccgagc cgatcaaggc cagcgcatcg aagttctaca agccggcggc
     4441 ctgatcgcat cgcctttgga gcgcgacagc tttatgtcat cgcgctccgg tttgttgttt
     4501 gagcatgatc tccgcgcaaa cgcgttcggc gtttgtcgcg aaggaaaagg cggtgcccgt
     4561 ttttccggat cgtgctcttg ttcattttcg aaacaagccc gggccaaagc gcccgggctt
     4621 gtttttggct cggcgtacgc tttcagatgc gctcggcggc cttgccaaag aaggccgttg
     4681 cacctagttt ccgccccgat cggcagcccg ttttcacccg ggtacgccgc ggatgcagtt
     4741 gtagctcagt tggttagagc gcctgactgt ggatcaggag gtcggtggtt cgagtccacc
     4801 caactgtacc atgtaagttt ttgaaataat aaggtaactt ttggttggac tggattggct
     4861 aatttgctga cccctaaaag gttccactca ggttccaaaa gcactttgtg tgttcgagct
     4921 gctgggcgca aatgagctga gtaggggccg tgcgccggat tgtccggtgc gaagcggcgc
     4981 cgcaagcacc agaaccaacc cgcgccgctc ctgccgaaga gcttccgatc cgcaaaaaga
     5041 tctcgatggt cgcgcccgtc acaggatgga catcgaagat gggcttctgc tgataccgcc
     5101 gatctgcgct cttgccccga cctccgtccg cgccgctagc gccagcccgc gcagataatc
     5161 agcagccttt gacgcctgac tgcacgcggt gaaaaaggct cgcttgtcgg ccttcaagag
     5221 ttcgatccag tggccgatgt aacccgcgtg ctggaaatcg ccgtcaaagc cgaactctgc
     5281 acacagaaac gcggcgcgca attcggcgat cagctcttcg acagcgtaat tccgcgagcc
     5341 aaaccggttc ttcaaatccc gatcaatggg gtgaccgctg gct
//
LOCUS       DATLJT010001022         1578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_756522,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001022 DATLJT010000000
VERSION     DATLJT010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756522"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VM910_23515"
     CDS             complement(<1..830)
                     /locus_tag="VM910_23515"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI65521.1"
                     /translation="MASFTITPLTARTGVEVVGLDFTKPIDTDDRAVLRRAFAEHHVL
                     VMRKQDFTPDQFETAAQLFGEIQLHDKRERHVPGHPDVDYVSNDQIENGRRIIPGETF
                     HTDHSNHPRPPKATMLFAVELPSSGGDTQYVNMHDAYDDLSAETKQRIDGLKAVHVYQ
                     SKYSPRPLGQITEESRSKLPPPGVHPLVRTHPENGRKALFLNPVRMEAIVGMEDKEAL
                     ALIDELMRHATQKKYEYRHKWRHGDWVLWDNRSVMHQANPDYDMRERRYLYRLMLKGE
                     T"
     gene            complement(934..>1578)
                     /locus_tag="VM910_23520"
     CDS             complement(934..>1578)
                     /locus_tag="VM910_23520"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI65522.1"
                     /translation="PTYPGQPPIIHGVWKTHEESFVESGNVHGNCVMFFSMPDHGGTH
                     IDAPRHFGMNGTPIDEYPLEYCYVKGICLDLRHIAPKAEITPSQLEEAVRKSGSAIPK
                     RGTVLLCTGHHARTFPTAAYSTDNPGVNVAATEWLARQGIVHFGIDSMRPGPMGAVNS
                     LVHKACLELDITHIESLCNLEALIGQGEFRFIGLPLKWRGGTGSPIRAVAVFEE"
BASE COUNT          248 a          460 c          547 g          323 t
ORIGIN      
        1 gtttcgccct tgagcatgag gcggtagagg tagcggcgct cgcgcatgtc atagtcgggg
       61 ttggcctgat gcatgacgct gcggttgtcc cacagcaccc agtcgccgtg acgccacttg
      121 tgacggtact cgtatttttt ctgggtggcg tgccgcatta gctcgtcgat cagcgccagc
      181 gcctccttgt cctccatgcc gacgatggct tccatgcgca ccggattgag gaacaacgcc
      241 ttgcggccgt tctcggggtg ggtgcgcacc aacgggtgga cgccgggcgg cggcagcttg
      301 ctgcgacttt cctcggtgat ttgcccgagc gggcgcgggc tgtatttgct ctgatagaca
      361 tgaaccgcct tgagcccgtc gattcgctgc ttggtttcag ccgacaggtc atcataggcg
      421 tcatgcatat tgacgtattg cgtgtcgccg ccgcttgagg gcagctcgac ggcgaacagc
      481 atcgtggctt tgggcgggcg cgggtggttg gaatggtcgg tgtggaacgt ctcgcccggg
      541 atgatgcgcc tgccgttctc gatctggtcg ttggacacgt aatcgacgtc ggggtggccg
      601 gggacgtggc gctcccgctt gtcatggagc tgaatctcgc cgaagagctg cgcggcggtc
      661 tcgaactggt ccggcgtaaa gtcttgcttg cgcatcacta gcacatgatg ctcggcgaag
      721 gcgcggcgca ggacggcgcg gtcgtcggtg tcgatcggct ttgtgaagtc gaggccgacg
      781 acctcgacgc cggtgcgagc cgtcagcggc gtgatggtga atgacgccat cgcatgaccc
      841 tccaggccac gggttcgtcg acttgacgct agagccggcc gtcgggaggg tcaagcaccg
      901 gcaaagccgc ccgattattc gatgcgtcct gccttactcc tcgaacaccg ccacggcgcg
      961 aatcggcgag ccggtgccgc cccgccattt gagcggcaag ccgatgaagc ggaattcgcc
     1021 ttggccgatc agcgcctcga gattgcacag gctttcgatg tgcgtgatgt cgagctcgag
     1081 gcacgccttg tgcacgagcg agttcacggc ccccatgggt ccgggacgca tggagtcgat
     1141 gccgaaatgc acgatccctt ggcgcgccag ccactcggtc gccgcgacgt tgacgccggg
     1201 attgtcggtc gaataggcgg cggtggggaa ggtgcgcgcg tgatgtccgg tgcagagcag
     1261 cacggtgccg cgtttcggga tggcgctgcc cgatttgcgc accgcctcct ccagctgcga
     1321 cggcgttatt tcggccttcg gcgcgatgtg gcgcagatcg aggcaaattc ccttcacgta
     1381 gcaatactcg agcgggtact cgtcgatcgg cgtcccgttc atgccgaagt ggcggggagc
     1441 atcgatgtgg gtgccgccgt ggtcgggcat ggagaaaaac atcacgcagt tgccgtgaac
     1501 gtttccagac tcgacgaagg actcctcgtg ggttttccag acgccgtgga tgatgggcgg
     1561 ctgtcccgga taagtggg
//
LOCUS       DATLJT010001023         4248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1677962,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001023 DATLJT010000000
VERSION     DATLJT010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677962"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VM910_23525"
     CDS             complement(<1..179)
                     /locus_tag="VM910_23525"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI65523.1"
                     /translation="MAKKKRHSRVEIVSKLAQANDLAAQGKLQTEIARTLGVSVMTLH
                     RWRKAPPEPQRASVA"
     gene            678..890
                     /locus_tag="VM910_23530"
     CDS             678..890
                     /locus_tag="VM910_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65524.1"
                     /translation="MGNVLSLQLARERAWQPVWNTETTGPGGGNASNQRLLEGLEAEN
                     AQLRRSVVELALQIQALRDVARTLTA"
     gene            complement(1080..1964)
                     /locus_tag="VM910_23535"
     CDS             complement(1080..1964)
                     /locus_tag="VM910_23535"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI65525.1"
                     /translation="MISEHDRQILSQLPVWDPTGDIVAQRRAWEELATVYNQDLPAIG
                     GLEKKVELRSGLRADVAVPKGAGPHPVVIYLHGGGWAFGSPTSHRKLGMQFAEAGYLT
                     IVLDYRLAPEHPFPAALEDTMFAIGWAADNARRWNSHGRRIAIGGDSAGGNLAVSAIT
                     SSAPELRSLVRAAVLFYGAYDLEATAERTRSLPGLQEQLSTYVSGKRDLFDDPHVSPL
                     KAVAPGILPPCFIVGAGDDSWCLADSLALAQALSAVASPYELHVMEGIPHGFMQMSTL
                     EAFRAAMRLMWEFLARNV"
     gene            complement(2146..2514)
                     /locus_tag="VM910_23540"
     CDS             complement(2146..2514)
                     /locus_tag="VM910_23540"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HVI65526.1"
                     /translation="MAAVNLKGMDVDALLALRADIDKRLSQKRSELEKQLSRLSAESG
                     HAPGISRTVFGRGSALKGRKVAPKYRGPGGETWAGRGARPRWLAALIKQGRKLDEFAI
                     DKTLTARKGRMGKKARRKKK"
     gene            2928..3422
                     /locus_tag="VM910_23545"
     CDS             2928..3422
                     /locus_tag="VM910_23545"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI65527.1"
                     /translation="MLARFLAGGACAVGMLASVCVTPAAAHPYDQYIRGRSFFGALFG
                     PSIARAVVSYPSNQKPGTIVVSTAQRRLYLVLGNGQALSYGIGVGKAGFAWSGVTQIT
                     DKREWPDWTPPDQMLRRRPDLPHHMAGGPDNPMGARGIYLGANEDVIDLYNRVRIGAT
                     VIVQ"
     gene            complement(3492..3851)
                     /locus_tag="VM910_23550"
     CDS             complement(3492..3851)
                     /locus_tag="VM910_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HVI65528.1"
                     /translation="MTDAAPWPTELRLHKDRKALTIAFDNGETFDLSAEYLRVRSPSA
                     EVQGHSPDERKTVAGKKDVAILEVNPIGNYAVRLVFDDMHSTGIYSWDYLLALGRNRE
                     ANWQEYLDDLAGKGLKR"
BASE COUNT          822 a         1283 c         1276 g          867 t
ORIGIN      
        1 attgcgaccg acgcacgctg aggctcaggt ggcgctttgc gccaccggtg caaggtcatg
       61 acactcacac cgagcgtgcg cgcgatttcg gtttgcaatt tcccttgcgc cgccagatca
      121 tttgcctgcg ccagctttga cacgatttcc accctcgagt gccttttctt tttggccata
      181 gcagcttgct ctcgccctcc tatgttcgtc tatcgcaagt tctaatcgac catgcgttag
      241 attgcccgga aaagatggtc aatccgtata ggcttaagtg acgcatgtcg atgtcagtga
      301 ttcacaacaa aatctaggtg tgaggaggcc ttcttattct aacaatgcgt tttctaacaa
      361 tgcgttgaat gcgattcgcc gggatcaaag gttctttgtt acctttctcg atacaattta
      421 aatgccatta caattaaacc tccagttgta ttagtaaaaa aagtatcgac ggaatcgcaa
      481 ccatttgtca cgccgcaaat tgaggttgag ggcgggtggt ctacggcgcg cagagacgaa
      541 atgtgaggcc tgcgtcgggg gtactttcct tggccaccaa agttgcaatg ggcgtcacgg
      601 gcgtgacgtc tccttgcctc gtgtaacggc accattcagc cgatggttgg ggacggtggg
      661 atagtagggg gagtgtcatg ggcaatgtac tgagcctgca gcttgcgcgt gaacgtgcgt
      721 ggcaaccagt gtggaatact gaaacaacgg ggcctggcgg ggggaacgcg agcaatcaga
      781 gattgcttga ggggctcgaa gccgaaaatg cacagcttcg acgcagcgtg gtggaactcg
      841 cgctccagat tcaagcattg cgcgatgttg ccagaacact gaccgcgtag ttggccatcc
      901 gatcatacgc gaccgcttgc ctggatgccg ggtgggaagc gcgttttggt gtgtcggtgt
      961 gaacggtcaa ggtagcaaga aataagtggc ctcagctggc ggcgctggga catgcccgca
     1021 tacttggtcc gggcggcttt gcgcgcgata actctcaact tgtcagagcc aagacgcgtt
     1081 cagacgttgc gcgccaggaa ctcccacatc aagcgcatgg ccgcgcggaa cgcctcgagc
     1141 gtgctcatct gcatgaaccc gtgcgggatt ccttccatca cgtgcagctc gtaagggctc
     1201 gcaacggctg atagggcttg ggcaagcgcc agcgagtcgg cgagacacca ggagtcgtcg
     1261 cctgctccga cgatgaagca cgggggaagt attcccggcg ccaccgcctt gagcggactc
     1321 acatgtggat catcgaacaa atccctcttg cccgaaacat acgtgctcaa ttgctcttgc
     1381 agcccgggca acgatctcgt gcgctcggcg gtcgcctcga gatcgtatgc accgtagaag
     1441 agtactgccg ctcgcaccag cgagcgcagc tcaggagcgc tcgaggtaat ggcgctgacc
     1501 gccaggttgc cgcccgcgga atcgccgccg atcgcgatcc gccggccatg gctgttccat
     1561 cggcgcgcgt tgtcagccgc ccacccaatc gcgaacatcg tgtcttccag tgccgctggg
     1621 aacggatgct cgggcgccag ccgataatcg agaacgatcg tcagataacc cgcttcggca
     1681 aactgcatcc ccagcttccg atgcgaggtt gggctgccga acgcccatcc accgccgtgc
     1741 agatagatga ccaccggatg tggaccggca cccttgggca ccgctacgtc ggcgcgaagg
     1801 cccgagcgca gctcgacttt tttttcgaga ccgccgattg ccgggagatc ctggttgtag
     1861 acggtcgcca gctcctccca agcgcggcgc tgcgcaacga tatcgccggt gggatcccag
     1921 accggcagtt gactgagaat ctgacggtcg tgttcgctga tcatagctcg tatcttcccg
     1981 ccttccgcaa gacgctagca catccgccaa agctgcgagg gccatcgtcg ttgcgcgccg
     2041 ggcggcggcg gccaccgatg aaataagtct atttgctgca aagtatcgaa tttcctttgc
     2101 caacaaacgc caaagttacg catccgcaac cgggcgactg ctggcctact ttttcttgcg
     2161 gcgggctttc ttgcccatgc gccccttgcg ggcggtcagc gtcttatcga tcgcgaactc
     2221 gtcgagcttg cggccttgtt tgataagcgc cgcgagccac cgcggtcgag cgccgcggcc
     2281 tgcccaggtt tcgccgccag gtccacgata tttaggcgcc actttgcgcc ctttcagcgc
     2341 agagccgcgg ccaaatacgg ttcgagaaat accgggagca tggccgcttt cagcgctcag
     2401 ccgagaaagt tgcttttcca gttcgctgcg cttttgacta agccttttgt caatgtcggc
     2461 gcgcagtgca agcaacgcgt caacgtccat accttttaga ttaaccgcgg ccatcaggcg
     2521 cctcccgttc gtggtgcgcc tcgatagaac tattcaatcg aagagaaagg caagcacgcc
     2581 aaagtggaat gcatggcaat gtacattaag ggcatacgtc ttttcgtttg gtattccggg
     2641 accgccgaca tctaacagag tcgcgcgctc gaaccgctgc aacaaagtcg cgcctttcgt
     2701 cgccctcacc cagctccgcg gcgacgccga tgcgccggcg tcagatcgat gatgccgccc
     2761 tgtcccgcgt tgcaaacgca cgggacaggt gcggcttttc tgcaacactt cgcgacaact
     2821 tgtacgcact tcagcctaaa tcatcgacag ccgcctcgcc gcttgtagaa tttcaagcgt
     2881 tgtaaaaggg ttcgggcttc aaacactcga tcttgcgagg ctttgccatg cttgcacgct
     2941 tcctcgctgg cggcgcgtgc gccgtcggca tgctggcttc cgtctgtgtg acgccggccg
     3001 cggctcaccc ttacgaccag tatattcgcg gccgctcgtt cttcggcgct ttattcggtc
     3061 cgtcgatcgc gcgcgcggtg gtgagttatc ccagcaatca gaagccgggc actatcgtgg
     3121 tcagcacggc gcagcgccgg ctctacctcg tgctcggcaa tggccaggcg ctgagctatg
     3181 gcattggagt cggcaaggcg ggcttcgcct ggagcggcgt cacccagatc accgacaagc
     3241 gcgagtggcc ggactggacg ccgcccgatc agatgctgcg tcgccggccc gatctgccgc
     3301 accacatggc cggcggcccc gacaatccaa tgggggcacg cgggatctat ctcggcgcca
     3361 acgaggacgt gatcgatctc tacaaccgcg tgcggattgg cgccacggtg atcgtgcagt
     3421 aaccgacgac gctggacgga cgatcagacg gcggacggtg cgacgcatcc atcctgcgtt
     3481 ccgggcgcgc gtcagcgctt caaacctttg ccggcgaggt cgtcgagata ctcctgccaa
     3541 ttggcctccc ggttgcggcc gagcgcgaga aggtagtccc agctgtagat gccggtcgaa
     3601 tgcatgtcgt cgaacacgag ccgcaccgcg taattgccga tcggattgac ctcgagaatg
     3661 gcgacgtcct tcttgcctgc gacggtcttg cgctcgtcgg gtgaatggcc ctgcacctcg
     3721 gcgctcgggc tcctgacgcg caaatattcg gcgctgaggt cgaacgtctc gccgttgtcg
     3781 aacgcgatcg tcagcgcctt gcggtccttg tgcaaccgca actccgtcgg ccagggcgct
     3841 gcgtcggtca tttcgagctc ctcgctcggg ggcagatcga cgtgaaagta gatcacgcag
     3901 aggccgcgcg atagctcgcg ccgaggttga gggccgacat gagggagcgc ccaaacggcg
     3961 gagcatctgc gccgcaatgc cgctgtccgg gctaaagcgt cgcattcggg aaagtgcaaa
     4021 cggctttccc atcacctgat cttctgatta catggcgaca aaataaccgg acggccatgc
     4081 gggattggag gccggtcgtc gcaaacgaac gcggagaggg gcgacagtca gatgcgaact
     4141 cgacctggcg ggatgcacgc tttcattccg atgtcttgat ctggccctgc aaggccgaat
     4201 gcagggtcga gcgcgcctgt gcgaaacgca tgcgtcgcag gcgtgcgc
//
LOCUS       DATLJT010001024         1786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1448533,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001024 DATLJT010000000
VERSION     DATLJT010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448533"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VM910_23555"
     CDS             <1..198
                     /locus_tag="VM910_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65529.1"
                     /translation="QVEVASVPFLKFVANKGIHDEAANYSFVLRGYVRHWLANVWSEY
                     DRIKLTDIVGSGKPAVRSSEK"
     gene            260..928
                     /locus_tag="VM910_23560"
     CDS             260..928
                     /locus_tag="VM910_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65530.1"
                     /translation="MPATPVFIVASPRPQVGKTLVARLLSEFLLLKNGRVTSFDINLK
                     EPSLLDYLPNLTETADIIDTFGKMALMDRVILDDGLAKVIDLGFHAFDEFFTMSEEIG
                     FLKEAARRNVVPIILFMADTDRVSSREHETLRRQIPPAQLITVDNEYVVRGELPAAMG
                     DGRVMPIPALPTFLKTYVDRLTFSFTDYLRQEKDSSTELHQWIRRNYFAFRELEMNLD
                     LQKS"
     gene            1073..>1786
                     /locus_tag="VM910_23565"
     CDS             1073..>1786
                     /locus_tag="VM910_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI65531.1"
                     /translation="MTTAKDGMELTRVGPGTVMGELMRCYWLPALMSSELVVDGPPIR
                     LMLLGEKLIAFRDSQGHVGVMDHRCPHRGASLFLGRNEESGLRCIYHGWKFDVAGNCL
                     DMPSVPPPDFKQKVKAHAYRTVERAGVVWVYMGTRAEAPPLPGFEILDVPEDEIRVAF
                     IQRECNYLQSLEGEIDTSHFGFLHAGHVDIDDLAEDEPVRYTIIDRAPRYHLRDAPWG
                     TQYAGYRPAGPDTTYWRFAN"
BASE COUNT          334 a          578 c          526 g          348 t
ORIGIN      
        1 caggtcgagg tcgcctccgt ccccttcctc aaattcgtcg ccaacaaggg catccatgac
       61 gaggccgcca actattcttt cgtgctgcgc ggctatgtcc ggcactggct cgccaatgtc
      121 tggagcgagt acgaccgcat caagctgacc gacatcgtcg gctccggcaa accggccgtt
      181 cgcagcagcg aaaaataatt ttggtcggcc aaaataattc ggctggatcg gacggcggtt
      241 tcgcctgata tagcgggtga tgcccgcaac ccccgttttc atcgtcgctt cgccccgccc
      301 ccaggtcggc aagaccctgg tggcacggct gctgagcgag tttctgctgc tcaagaacgg
      361 tcgggtgacg tcgttcgaca tcaacctgaa agagccctcg ctgcttgatt acctgccgaa
      421 cctcaccgag accgccgata tcatcgacac tttcggcaag atggcgctga tggaccgcgt
      481 catcctcgac gacggcctcg ccaaggtgat cgatctgggc tttcacgcct tcgacgagtt
      541 cttcacgatg tcggaagaga tcggctttct gaaggaagcg gcgcgacgca acgtcgtccc
      601 gatcatcctc ttcatggccg ataccgaccg cgtctcctcg cgcgaacacg agacgttgcg
      661 gcgccagatt ccgcccgccc agctcatcac cgtcgacaac gaatatgtgg tgcggggcga
      721 gttgccggcc gcgatgggcg atggccgcgt catgcccatt ccggcgctgc cgacattcct
      781 gaagacctat gtcgaccggc tgaccttttc cttcaccgac tatctgcgcc aggaaaaaga
      841 ctcctccacc gagttgcatc agtggatccg gcggaattac tttgccttcc gcgaactgga
      901 aatgaacctc gatttgcaga agtcttgagg ccccttgcgc ccggcgcggg atcgccctgc
      961 ggcgggactg acctgcggct gtcgacagcc atggccgatt gtggcagatt ccttccaata
     1021 aagcagtcgc ggcttctcaa gggcggtcaa ccgaccgcat cgggaggatg agatgacgac
     1081 ggccaaggac gggatggaac tgacgcgcgt cggccccggc actgtcatgg gcgaattgat
     1141 gcgctgctac tggctccccg cactgatgtc gtccgagctc gttgtcgacg gtcccccgat
     1201 ccgtttgatg ctgctcggcg aaaagctcat cgccttccgc gacagccagg gccatgtcgg
     1261 cgtgatggat caccgctgcc cgcatcgcgg cgcctcgctg tttctcgggc gcaacgagga
     1321 aagcggactg cgctgcatct atcacggctg gaaattcgat gtcgccggca actgcctgga
     1381 catgccgagc gtgccgccgc cggatttcaa gcagaaggtc aaggcacacg cctaccggac
     1441 ggtcgagcgg gccggtgtgg tctgggtcta catgggcacg cgcgcggaag cgccgccgct
     1501 gccgggcttc gaaattctgg acgtcccgga agacgagatc cgcgtcgctt tcatccagcg
     1561 cgaatgcaat tacctgcagt cgctcgaagg cgagatcgat acctcgcatt tcggcttttt
     1621 gcatgcaggc catgtcgaca tcgacgacct cgccgaggac gaaccggtgc gctataccat
     1681 catcgatcgc gcgccgaggt atcatctgcg cgatgcgccc tggggaacgc aatatgccgg
     1741 ctatcgcccg gctggccccg acaccaccta ttggcgcttc gccaac
//
LOCUS       DATLJT010001025         6307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_70041,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001025 DATLJT010000000
VERSION     DATLJT010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70041"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VM910_23570"
     CDS             <1..247
                     /locus_tag="VM910_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HVI65532.1"
                     /translation="PFLLAAMVLLAASVSLRFFRFGGVQKMVLSGVGAGFLLYVLSKV
                     TEDLSKAELMHPIAAAWLPVVVGGLTGFLALLYQEDG"
     gene            253..2742
                     /locus_tag="VM910_23575"
     CDS             253..2742
                     /locus_tag="VM910_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HVI65533.1"
                     /translation="MVAALQLLFALPRRTCVRRERSVMAVTARLLASILVLLALGALQ
                     LVTADSASAQFTYNPRPPQPPRAPRVAGDGQMLVQAVEVDYDYNNNRVSAVGNVQLFY
                     NGTSVEADKVVYDQKTKRLYAEGNIRMTDADGKITYANTMDLSDDYRDGFVDSLRVDT
                     ADATRMAATRADRSSGNYTVFENGVYTACAPCQDDPKKPPLWQVKGARIIHDQQEKML
                     YFETAQIEFFGVPVAYLPYFSTPDPTVKRKSGFLMPGYFPGTGQTGFGMEIPYYWAIA
                     PDYDATFTPRILSRQGVLMQAEFRQRLIDGSYQIRAYGIDQLDPGAFAGQPGDRQFRG
                     GLDTKGQFAINDKWVWGWDGVILSDYAFFNDYRLAQYRDPMSAFLSLPTEAISQLYLT
                     GVGNRSYFDLRSIYYLSYSGNQQQVPVVYPVLDYSNVFNYPIFGGEVSYKTNFTNLTR
                     NDAVFDPITNLANFAGLCTPTSADPLARTPSQCLLRGFPGTYTRLTVEAQWRRSFTDS
                     AGEIWTPFASFRADAINADVSNQPGVSNFLPVGDTQALRPMPTVGLEYRYPFINVQPW
                     GSTTVEPIAQVIIRPNETYAGQLPNEDAQSLVYDASNLFSVDKFSGYDRVEGGGRANV
                     GMQSTTQFDQGGTVKALFGQSYQLFGLNSFAVKDATNTGLDSGLAKPLSDYVTSVSYS
                     PNRTYTFSVRSRFDEQTWNVERFEAEAKANFDRWSIGLTYGNYAAQPDLGYLTRREGL
                     LASGSVKITANWVATGAARWDLVANNINQYAVGAGYVDDCFVMAANYVTSYNYSAGTA
                     PPVLGHAYMLQIGLRTLGNTTSSGVGGAGLQ"
     gene            2829..3764
                     /locus_tag="VM910_23580"
     CDS             2829..3764
                     /locus_tag="VM910_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVI65534.1"
                     /translation="MSTTFSHRLLLLVVSCVVGAFLACATPARAQTVTVMVNGEPITE
                     YDIDQRTKLNFLTTHKQSPRQEVVNELIDEKVKIKEAKKFGVDPTSSDIDQAYGQMAG
                     RMRITSDQLTKSLEGQGIRPDTLKSRIKAEMVWGSLVRGRYKERLQVGEKDVAAAVQA
                     DGGESQQQADAFEYRMQPVVLIVPHGASQSAIELRQKEAETLRARVQTCGEASAYFKT
                     MANAAIREPLIRTSADIPANLRKVLDDTPVGHLTPPEVTKQGIEMVALCGRKPTTIDT
                     PKKKEMRDKMYAEKYLATSNAYLQEIRKAAMIEYR"
     gene            3904..4947
                     /gene="pdxA"
                     /locus_tag="VM910_23585"
     CDS             3904..4947
                     /gene="pdxA"
                     /locus_tag="VM910_23585"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVI65535.1"
                     /translation="MNHELRKVDTRPLALTLGEPAGIGPDIAIKAWLRREELKLPAFY
                     LLGDRNFLERRAAALGLHVRLADVDANTAGAAFADALPVVSTGQAATAEPGQPDAQSA
                     QAALSSIRQAVRDVRSGLASAVVTNPIAKSVLYRAGFRHPGHTEYLAELTADKGQTPQ
                     PVMMLWSPVLAVVPVTIHASLREAIGRLSTELIVSTARIVADALKTRFGIPQPRLAVS
                     GLNPHAGEEGTLGREDETIVAPAVDILRAEGLTVKGPLPADTMFHDAARKNYDCAICM
                     YHDQALIPIKTLAFDDAVNVTLGLPFIRTSPDHGTAFDIAGTGRANPASLIAALRLAA
                     RIAATPAPASAPP"
     gene            4944..5810
                     /gene="rsmA"
                     /locus_tag="VM910_23590"
     CDS             4944..5810
                     /gene="rsmA"
                     /locus_tag="VM910_23590"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVI65536.1"
                     /translation="MSAIDDLPPLREVIREHALSARKSLGQNFLLDLNLTARIARAAG
                     PLEGATVVEIGPGPGGLTRALLALGATHVIAVEHDARAIPALEIIGRRYPGRLEIVCA
                     DAQTFDPRPYLGSTKSKIVANLPYNIATALLIGWLSIEPWPPWYDMMVLMFQREVAER
                     IVARENDEAYGRLGVLANWRCETKILFDISPAAFVPQPKVTSSVVRLLPRAKPEACER
                     RALEQVAAAAFGQRRKMLRQSLKSLPADPVRLAAAAQIDPTRRAETIPISGFVAMARE
                     LANIRGEKQNTS"
     gene            5823..>6307
                     /locus_tag="VM910_23595"
     CDS             5823..>6307
                     /locus_tag="VM910_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI65537.1"
                     /translation="MTLMRRQSLVKFDAPLCETIVDTPKPQRKEVLVRIERCGLCHSD
                     LHIQDGYADLGGGRRLDTTRGMTLPFTLGHEIAGVVEEVGPDVGKELVGARKAVFPWI
                     GCGQCRDCLNGDENLCLKQRFLGVSIDGGFASHVLVPDAKYLLDYDPLPVNQAATLMC
                     SG"
BASE COUNT         1220 a         2025 c         1899 g         1163 t
ORIGIN      
        1 gccgtttttg ctggccgcga tggtcttgct ggctgcatcc gtcagcttgc gcttcttccg
       61 attcggcggc gtacaaaaga tggttttgag tggcgtgggc gcgggctttc tgctctatgt
      121 tctgtccaaa gttaccgagg atttgagcaa ggccgagttg atgcatccga tcgctgcggc
      181 gtggctgccc gtggttgtgg gcggcctaac cggctttttg gccttgttat accaggagga
      241 cgggtagtgg ccgtggtcgc cgccctccag cttctgttcg cgttaccgcg gcgcacctgc
      301 gtgcgccgcg aacgatcggt tatggccgtg acggcgcgcc tgctcgcatc gatcctcgtg
      361 ctgctcgccc ttggtgcgtt gcagcttgtg acggcggact ccgcgtcggc ccagttcacc
      421 tacaatcccc gtccgccaca gccgccccgc gcgccgcgcg tcgccggcga cggacagatg
      481 ctcgtgcagg cggtcgaggt cgactacgat tacaacaaca accgcgtgtc ggcggtcggc
      541 aacgtgcagc tcttctacaa cggcaccagc gtcgaagccg acaaggtcgt ctacgatcaa
      601 aagaccaaac gcctttacgc cgaaggcaac atccgcatga cggatgccga cggcaagatc
      661 acctacgcca acaccatgga tctgagcgac gactaccgcg acggattcgt cgattcgctg
      721 cgggttgaca ccgcggatgc cacgcgcatg gcagcaacgc gcgccgatcg ctcgagcggc
      781 aactacacgg tttttgaaaa cggcgtttac accgcctgcg caccctgcca ggacgatccg
      841 aaaaagccgc ctttgtggca ggtcaagggt gcccgcatca tccatgacca gcaggaaaag
      901 atgctgtatt tcgagacggc gcagatcgaa tttttcggcg tgccggttgc ctacctgccg
      961 tacttctcga cgccggatcc gacggtcaag cgcaagagcg gtttcctcat gccgggttac
     1021 ttcccaggaa ccgggcagac cggcttcggc atggaaatcc cctattattg ggcgatcgca
     1081 cccgattatg acgcgacctt cacgccgcgc atcctgagcc ggcagggcgt gctgatgcag
     1141 gccgaattcc gtcagcgcct gatcgacggg tcgtatcaga ttcgcgccta cggcatcgat
     1201 cagctcgatc cgggcgcttt cgccggtcag cccggtgacc gccaattccg cggcggcctc
     1261 gataccaagg gacagttcgc gatcaacgac aagtgggtct ggggctggga cggcgtcatc
     1321 ctttcggact acgctttttt caacgactat aggctcgcgc aataccgcga tcccatgagc
     1381 gccttcctca gtctgccgac cgaagcgatc tcgcagctct atctgaccgg cgtcggcaac
     1441 cgcagctatt tcgatctgcg ttcgatctac tatctgagct attcgggtaa ccagcagcag
     1501 gtgccggtcg tctaccccgt gctcgattat tcgaacgtct tcaactatcc gatcttcggc
     1561 ggcgaggttt cttacaagac caacttcacc aacctgacgc gcaacgacgc ggtgttcgac
     1621 ccgatcacga atttggcgaa cttcgccggc ctgtgcacgc cgacctcggc cgacccgctg
     1681 gcgcggacgc cgagtcagtg tctgttgcgc ggtttccccg ggacgtacac gcggctgacg
     1741 gtggaagcgc agtggcggcg atcgttcacg gattccgcgg gcgaaatctg gaccccgttc
     1801 gcgagcttcc gtgcagacgc gatcaatgcg gacgtttcca accagcccgg cgtctccaac
     1861 ttccttcctg tgggcgatac ccaggcgctg cggccgatgc cgaccgtcgg cctcgaatac
     1921 cgctatccct tcatcaacgt acagccgtgg ggctccacga ccgtggagcc gatcgcgcag
     1981 gtcattattc gtccgaatga gacctatgcc ggccagcttc ccaacgaaga tgcacagagc
     2041 ctagtttacg acgccagcaa cctgttcagc gtcgacaagt tctcgggcta tgaccgcgtc
     2101 gagggtggcg gacgcgccaa tgtcggcatg caatcgacca cgcaattcga ccagggcggc
     2161 accgtcaaag cgttgttcgg acagtcctac caactgttcg gcctgaactc ctttgccgtc
     2221 aaggatgcca ccaataccgg cctcgattcc ggccttgcga agccgctctc cgactacgtc
     2281 accagcgtta gctactcgcc gaaccggacc tatacgttca gcgtccgctc gcgctttgac
     2341 gagcagacct ggaatgtcga gcgcttcgag gccgaagcga aggcgaattt cgatcgctgg
     2401 tcgatcggcc tgacctacgg caactacgca gcgcagcccg atctcggtta cctgacccgc
     2461 cgcgaagggc ttttggcctc cggctccgtc aagatcaccg caaactgggt ggcgaccggc
     2521 gcggcccgct gggatctggt ggcgaacaac atcaaccagt acgcggtcgg cgcgggctat
     2581 gtcgacgact gcttcgtgat ggccgccaac tacgtcacgt cctacaatta ttcggcaggc
     2641 acggccccgc ccgttctagg tcacgcctac atgctgcaga tcggccttcg gacactcggc
     2701 aataccacct catcgggcgt cggcggggcc gggctgcaat agccggacgg cctccggcaa
     2761 agcggtgtcc gcgccagtcc caaggaatcg aaacggttat cggatcggtt gttacagcga
     2821 aaatgatcat gtcgaccaca ttctctcatc gcctgctgct cttggtcgtg agctgtgtcg
     2881 tgggagcttt tcttgcgtgc gcaacacctg cacgcgcgca gacggtgact gtgatggtca
     2941 acggtgagcc catcactgag tacgatatcg accagcgtac caaactcaat ttcctgacca
     3001 cgcacaagca gtcgcctcgc caggaggtgg tcaacgagct gatcgacgaa aaggtgaaga
     3061 tcaaggaagc caagaaattc ggggtcgatc ccacctcctc cgatatcgat caagcctatg
     3121 ggcaaatggc tgggcgcatg cggatcacat ccgatcaatt gacgaaatcg cttgaaggcc
     3181 agggcatccg gcccgacacg ctgaaatcgc gcatcaaggc cgaaatggtg tggggcagtc
     3241 tggtacgcgg gcgctacaag gagcggctgc aggtcggcga gaaggatgtc gccgccgcgg
     3301 tgcaggcaga cggcggcgag agtcaacagc aggccgacgc ctttgaatac aggatgcaac
     3361 ccgtcgtgct gatcgttccg cacggcgcct cgcagagcgc gatcgagctt cgtcagaagg
     3421 aggccgaaac gttgcgcgca cgcgtccaga cctgcggcga ggccagcgcg tatttcaaga
     3481 cgatggcaaa cgccgcgatc cgcgaaccgc tcatcaggac gtcggccgac atcccggcga
     3541 atttgcgcaa ggtgctcgac gatacgccgg ttgggcattt gacgccgccg gaagtgacca
     3601 agcagggcat cgagatggtg gcattgtgcg ggcgcaagcc aaccaccatc gatactccga
     3661 agaagaagga aatgcgcgac aagatgtatg cggaaaaata cctcgcgacg tcgaatgcct
     3721 acctgcagga aatccgcaaa gccgccatga tcgaatatcg ctgacggtgc cggccgcccg
     3781 attgaagttt ccgcatagct cgtggcaaat tcgatccagg aacttcaaat tcaaatgcgg
     3841 cactggaatt taggaattgc cggtgcccat ggcttccgaa gttcgtgaaa actggatgtt
     3901 gcgatgaacc acgaactccg gaaagtggac accaggccgc tcgcgctgac actcggcgag
     3961 ccggccggca tcggtcccga catcgccatc aaggcctggc tgcgcaggga ggagctgaag
     4021 cttcctgcat tctatttgct tggcgaccgc aacttcctgg agcggcgcgc ggcggccctc
     4081 ggcctccacg tcaggctggc cgatgtcgat gctaacaccg ccggcgcggc gtttgcggac
     4141 gcgctgcccg tggtttcgac ggggcaagcg gcaaccgccg aacccggtca accggacgcg
     4201 caaagcgcgc aagccgcgct gtcttccatt cgccaggcag tcagggacgt gcgcagtggg
     4261 ctggcaagcg ccgtcgtgac caatccgatc gccaagagcg tgctctaccg cgccggcttt
     4321 cgccatcccg gacataccga atatctggcc gagctcacgg cggataaagg gcaaacgccg
     4381 caacccgtca tgatgctttg gtcgccggtg ctggccgtcg tgcccgtgac gatccacgca
     4441 tcgctgcgcg aggcgatcgg ccgactctcg accgaattga tcgtgagtac cgctcgcatc
     4501 gtagcggacg ccttgaaaac ccgtttcggc atcccgcagc ctcgtcttgc cgtctccggc
     4561 cttaacccgc atgccggcga agaaggcacg ctcggaagag aggacgaaac catcgtggca
     4621 ccggccgtag acatcctcag ggccgagggc ctaaccgtca aagggccgtt gccggcagat
     4681 accatgtttc acgacgcggc gcgcaaaaac tacgactgcg cgatctgcat gtaccacgac
     4741 caggcgctga tcccgatcaa gaccctggcg ttcgatgatg cagtcaatgt cacgctcgga
     4801 ctgcctttca tccgcacctc gcccgaccac ggcacggcct tcgatatcgc cggcaccggc
     4861 cgcgccaacc ccgcaagcct gatcgcagcg ttgcggcttg ccgcgcgcat cgccgcaacg
     4921 cctgcgccag cctcggcacc gccatgagcg cgatcgacga cctcccgccg ctgcgcgagg
     4981 tcatccgcga gcacgcgctg tcggcgcgca aatcgttggg ccagaatttt ctgctcgacc
     5041 tcaacctcac cgcgcggatc gcccgcgctg cgggacccct tgaaggggca accgtcgtcg
     5101 agatcggccc gggtcccggc ggcctgaccc gcgcactgct ggctttgggc gcgacgcacg
     5161 tgatcgcggt cgagcatgac gcgcgcgcca ttcccgcgct ggagatcatc ggccggcgct
     5221 atccaggtcg actggaaatc gtctgcgcgg acgcacagac cttcgatccg cggccttatc
     5281 tcggatcgac caagtcaaaa atcgtcgcga acctgcccta caatatcgcg acggcgttgc
     5341 tgatcggctg gttgtccatc gaaccctggc cgccctggta cgacatgatg gtcttgatgt
     5401 ttcagcgcga agtggccgag cgcatcgtcg cgcgcgagaa tgacgaagcc tatggccggc
     5461 tcggtgtgct cgcgaattgg cgctgtgaga ccaagatcct gttcgacatc tcgccggcgg
     5521 ctttcgtgcc ccagccgaag gtcacctcgt ccgtcgtgcg gctattgccg cgcgcaaagc
     5581 ccgaggcctg tgagcgccgc gcgctggagc aggtcgccgc cgcagcgttc ggccagcggc
     5641 gaaaaatgtt gcggcagagt ctcaaatcat tgcccgccga tccggtgcgg cttgcggccg
     5701 cggcacaaat tgatccgaca cgccgcgcgg aaacaattcc gatttcaggc tttgttgcca
     5761 tggcgcgtga attggccaat attcgcggcg agaaacagaa cacatcctga aggagaagaa
     5821 gaatgacgct gatgcgtcgg caatcgctcg tcaaatttga cgcccccctc tgtgaaacca
     5881 tcgtcgacac gccgaagccg caacgcaaag aggtgctcgt gcgcatcgag cgctgcggac
     5941 tgtgtcattc cgacctgcat atccaggacg gctatgccga tctcggtggc ggcaggcggc
     6001 tcgatacgac gcgcggcatg acgctgccat tcacgctcgg tcatgagatt gcgggcgtcg
     6061 tggaggaagt cggacctgat gtcggcaagg agctggtcgg cgcaaggaag gcggtgttcc
     6121 cctggatcgg ctgcggccag tgccgcgact gtctcaacgg cgacgaaaat ctctgcctga
     6181 agcaacgctt tcttggcgta tccatcgacg gcgggttcgc cagccacgtg ctggtgcccg
     6241 atgcaaaata cctgctggat tacgaccccc tgcccgtcaa tcaggccgcg acgctgatgt
     6301 gctccgg
//
LOCUS       DATLJT010001026         5339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_757119,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001026 DATLJT010000000
VERSION     DATLJT010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757119"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="VM910_23600"
     CDS             complement(<1..1215)
                     /locus_tag="VM910_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI65538.1"
                     /translation="MVSSGGLSCGAHMAPHEVHLAQPTARPPDYHHALSAARHRVTSA
                     NQWTESQLMGRRWPIGCVALEITQRCNLDCTACYLSEHSEAVKDIPLEELFRRIDLIF
                     DHYGPNTNVQVTGGDPTLRKRDELVAIVRRLSQKSMRPALLTNGIRAKRELLVELAKA
                     GLVDVAFHVDITQGRKGYGSEVALNEVRKEYIERARGLPLSVMFNTTVVDRNFEEISE
                     IVEFFVRNCDVVRLASFQLQADTGRTVLGRRSDAITIQSVQEQIEKGARSPISFDTLR
                     VGHARCNRYGMIFVANNRAYDALDDRRSLEALLKRMPLLSFDRRSRSKVIATFVKGIL
                     TSPYLSFKAAGWLLRKAWQAKSDLWAARGRVNKLSFVIHNFMDACRLERDRVDACAFM
                     AMTGRGPISMCLH"
     gene            complement(1290..1814)
                     /locus_tag="VM910_23605"
     CDS             complement(1290..1814)
                     /locus_tag="VM910_23605"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI65539.1"
                     /translation="MTKHEFKLHTIETAPEASRNALTEVKKKFGRVPNFFGITAESPA
                     ATNAYLSLSKIFQSTALTPAEQQIVILAASVENKCDYCVAAHSRGAKMAGVPEDAINA
                     IKAKAPLEDAKTEALRCLVSQIVDKHGWRSDADVQAFLNQGYSRSQLLDVMVGVSMKT
                     LSNYVNHLTGPPIE"
     gene            2103..2681
                     /locus_tag="VM910_23610"
     CDS             2103..2681
                     /locus_tag="VM910_23610"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI65540.1"
                     /translation="MLLFWRKGYGATSIDDLEKATRLRRGSLYNAFGDKQGLFVAALK
                     RYAAIITEERIKRLSNPDPRKGIEGFFDILIAQLSEPSRPRGCLHTNTSLEFPHAPDE
                     VLRIIAERTAAIEGAIYIMLRRAQAECMLDPKADARALARFYLGVAKGITVLHKVSGD
                     ILALRDIVKVAISKWPHPEGKRPGTSKRDRRV"
     gene            3046..3507
                     /locus_tag="VM910_23615"
     CDS             3046..3507
                     /locus_tag="VM910_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HVI65541.1"
                     /translation="MTAKNWLKGMVAGFIATVVLSVLMLIKQRMGLVPQLNPIEMLAK
                     MAGGSMPAVGWVPHFLIGTVVWGSLFPIFDARVPAGSHWLNGILFGIGAWILMMVLVM
                     PMAGAGLFGMALGIMVPIATLVLYLIYGAVLGGTYGLEHPDPLPQFQVTHR"
     gene            3545..3790
                     /locus_tag="VM910_23620"
     CDS             3545..3790
                     /locus_tag="VM910_23620"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65542.1"
                     /translation="MTERAAREAGRRAKTARFDMRRKGKAVGVGETAGSINVGVEAET
                     DRLFGAAVPANDGELDLMNAGALYQAHSVESAGVDHA"
     gene            3783..4634
                     /locus_tag="VM910_23625"
     CDS             3783..4634
                     /locus_tag="VM910_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65543.1"
                     /translation="MRNSQQDIGVRTGAPERTIAKVLAGQKALVTGANSGIGQAAAIA
                     LGQAGADVVVNYVTGEAQAVVDAICRAGAEAYAHKADVSQEDQVEDMFRHMVERFGTV
                     DILVNNAGLQRDAAFHEMTLAQWDTVIGINLTGQFLCARAAVREFLRRGIVPAVSCAA
                     GKIICMSSVHEVIPWAGHANYAASKGGIMLMMKSLAQEVASHRIRVNSIAPGAIRTPI
                     NTSARSTEKAYDQLMTLVPYKRIGEPEDIARAVVWLASDDSDYVVGTTLFVDGGMTLY
                     PGFTEGG"
     gene            4647..>5339
                     /locus_tag="VM910_23630"
     CDS             4647..>5339
                     /locus_tag="VM910_23630"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HVI65544.1"
                     /translation="MASGAIEAVRGHWPEYLIEAAGLALFMISAGAFGTLLEYPQSPV
                     HGMLPDPLVRRALMGIAMGATAIGLIYSPWGRQSGAHFNPAVTLAFSRLGKVAPWDGV
                     FYVIAQFVGGLAGVLAISAVLGNAFREPPVEFVVTVPGFAGPLITFLAEFAISCGLML
                     AVLYTSNRIHLMRYTGLFAGALLAGYITVEAPFSGTSMNPARTVASAALSGVWDGVWI
                     YFSAPLLGMLIAV"
BASE COUNT         1001 a         1571 c         1684 g         1083 t
ORIGIN      
        1 gtgcagacac atcgagatgg ggccgcgtcc cgtcatcgcc atgaaagcgc acgcgtcgac
       61 ccggtcccgt tccaagcgac acgcatccat gaaattgtgg atgacgaacg acagcttgtt
      121 cacgcgaccg cgcgccgccc agagatcgga cttggcctgc cacgccttcc ggaggagcca
      181 accggcagct ttgaatgaaa ggtatgggct ggttagaatc cccttcacga aagtggctat
      241 gaccttggac cggctgcggc ggtcgaaact caacagcggc atgcgcttga ggagggcctc
      301 caacgatcgc cggtcgtcga gcgcgtcgta ggctctgttg ttggccacga agatcattcc
      361 atagcgattg cagcgcgcgt gtccgacgcg gagagtgtcg aaggagattg gggaccttgc
      421 gcccttctcg atctgctcct ggaccgattg gatagtgatc gcgtcgcttc ggcgtcccag
      481 gacggtgcgg ccggtgtcgg cctggagttg gaaggacgcc aggcgcacga cgtcacaatt
      541 gcgaacgaag aattcgacga tctcggatat ttcctcgaaa ttgcggtcga cgacggtcgt
      601 attgaacatc accgacaacg gcagtccacg tgcccgttcg atgtattctt tgcgcacttc
      661 gttgagcgcc acttcactgc cgtacccttt gcgtccctgc gtgatgtcga cgtgaaaggc
      721 aacgtcgacg agacccgctt tcgcgagttc gaccagcaat tcgcgcttgg ctcggatgcc
      781 attggtgagc agcgctggcc gcatgctttt ttgcgataac ctgcgcacga tggcgaccaa
      841 ctcgtcccgt ttgcgaagcg tgggatcgcc gcccgtcacc tgtacattgg tattggggcc
      901 atagtgatcg aaaatcagat cgatgcggcg gaacaattcc tcgagtggaa tgtccttcac
      961 ggcttcagag tgctccgaca ggtagcaggc tgtgcagtcg agattacagc gctgcgtgat
     1021 ctcgagcgcg acgcatccga tcggccagcg acgccccatg agctgacttt cggtccattg
     1081 gttggcggac gtgacgcggt gccgcgccgc gctgagtgca tgatggtaat cgggaggccg
     1141 cgccgtgggc tgcgcgaggt gaacttcgtg cggtgccata tgcgcgccgc atgacaggcc
     1201 gccgctcgac accatgctag ccgccaatgc tctaaataaa gtcacctgat gccccaatga
     1261 cgtttcgccg cgcggatcga cgtaggtagc tactcgatgg gaggaccggt gaggtggttg
     1321 acgtagttgc tcagcgtttt catggacacc ccgaccatca cgtccagcaa ctgcgatcgg
     1381 gaatagcctt gattgaggaa cgcttgaacg tcggcgtccg agcgccatcc gtgcttgtcg
     1441 acgatttggc tgacaaggca gcgtagcgcc tcggtctttg catcctctaa cggggctttg
     1501 gctttgattg cattgatcgc gtcctcgggc acccccgcca tctttgcgcc tctgctgtgg
     1561 gcggcgacgc agtaatcaca cttattttcg acgcttgccg ccagaatgac gatctgctgc
     1621 tcggctggcg tgagcgcggt gctttggaaa attttggaca gcgagaggta ggcattggtc
     1681 gcggccggcg attcggcggt gatgccaaag aaattcggca cgcgaccgaa cttcttcttg
     1741 acctcggtta gagcattgcg cgaggcctca ggcgccgttt cgatagtgtg gagtttgaac
     1801 tcatgtttgg tcatgatccc tcctggtccg atgtcacgcg aaacattgag ggcacgattt
     1861 cgatccaaat cgcggaccgt gatgagacgg catctagccg acggagctca cggtaaacat
     1921 tatagtcctg gtacgatcag tcaaagataa tttcggccgc gtcagctaag aacggctggc
     1981 tgcctcaaac acattcatgt tacctagtga gctccgttac cggcgccgcc caacgaaacg
     2041 cgaaaggcga cactatgcca ggcaccagac agttcgatca tgacacggtg gtcgagcgcg
     2101 ccatgctgct gttctggcgc aaaggctacg gcgcaacatc aatcgatgat ctggaaaagg
     2161 cgacccggct acgccggggc agcctttaca acgcattcgg cgacaagcag gggctattcg
     2221 tcgccgcgct caagcgctac gcggcaatca tcaccgagga gcggattaaa cgtttatcca
     2281 acccggaccc gcgtaaggga atcgaaggat ttttcgatat tctcatcgca caattgagcg
     2341 aaccgagccg gccgcgcggc tgcctccaca cgaatacctc actcgagttc ccccacgcgc
     2401 cagacgaagt gcttcgcatc atcgccgagc gaaccgcagc gatcgaaggt gcaatctata
     2461 ttatgctgcg gcgcgcccag gccgaatgca tgcttgatcc gaaggctgac gcgcgtgcgc
     2521 tggcccgctt ctatcttggt gtcgcgaagg gcatcaccgt gctgcacaaa gtctccgggg
     2581 atatcttggc attgcgcgac atcgtcaagg tcgcgatcag caaatggccg catcccgagg
     2641 gcaaacggcc gggcacaagc aaacgtgatc gtcgcgtttg aatccggttc ttgaccggtc
     2701 attccagcat tgcaaggatc gcctgtcgcg gcatggaggc cgtcgatggg cggcaatccg
     2761 aagttccgtc acaatcagtc atcgccgccg caccggacga aggatccgcg acggacgcgt
     2821 cgcgccattg cggggttcct gctggtggga tcatccgcgg gaagccgctt tgtcggggcc
     2881 gcgcacgctc aagccgttcc cacaaccgtg gagctggatg ggccggatca cctatcccgg
     2941 cgtcacgtga aggtgcgccg ccccagttgt tgtgggggtg agttttgaga actggtggaa
     3001 gcgccgcttt attccgcaga cccaacagcg cctgggagat tcgccgtgac agcaaagaac
     3061 tggctcaaag ggatggttgc cggctttata gcgacggtgg tgctatccgt cttaatgctc
     3121 atcaagcagc gaatggggct ggtgccgcag ctcaacccaa tcgaaatgtt ggcgaaaatg
     3181 gctggcggat cgatgccggc tgtcggctgg gtcccccact tcctgattgg aaccgtggtg
     3241 tggggtagct tgtttccgat cttcgatgcc agagttcccg ccggcagtca ctggctgaac
     3301 ggcatcctgt tcggaattgg cgcgtggatt ttgatgatgg tccttgtaat gccaatggca
     3361 ggggcaggtc ttttcggcat ggcgctgggc atcatggtgc ccatagcaac gctcgttctc
     3421 tacctgattt atggagcggt actgggcggc acctatggac tagagcatcc cgatccgctg
     3481 ccgcagtttc aggtgacgca tcggtagctc ttgatggagt ggacaccgag cgcggcgcgt
     3541 cgtcatgacc gagcgcgccg cgcgcgaggc cggccgcagg gccaagaccg cccggttcga
     3601 catgcggcgg aaaggcaaag ccgtaggcgt cggcgaaacc gcaggctcca tcaacgtcgg
     3661 ggtcgaggcc gagactgatc gcctgttcgg cgcggccgtg cccgcgaacg acggcgagct
     3721 cgatctgatg aatgcgggag cgctttatca ggcgcactcg gttgagtcag cgggagtcga
     3781 tcatgcgtaa ttcccaacaa gacatcggag tgcgaaccgg cgcccctgag cggaccattg
     3841 cgaaggttct ggctggccag aaggcactgg tcaccggcgc caattccggc atcggccagg
     3901 cggctgccat cgctctcggg caagccggtg cagatgtcgt ggtcaattac gtgaccggcg
     3961 aggcccaggc ggtggtcgac gcgatctgtc gagcgggcgc cgaggcttat gcacacaaag
     4021 ccgacgtgtc gcaggaggac caggtcgagg acatgttccg tcatatggtc gaacggttcg
     4081 gcaccgtcga tatcctggtt aacaacgccg gactgcagcg ggacgcggcg tttcacgaga
     4141 tgaccttggc gcagtgggac accgtcatag gcatcaattt gaccggtcag ttcctctgcg
     4201 cgcgggcggc ggtgcgtgag ttcctgcgcc gcggcatcgt gccggcggtg tcctgtgccg
     4261 ccggcaaaat catctgcatg agctcggtgc acgaggtgat cccgtgggcc ggtcacgcca
     4321 attacgccgc ctcgaaaggc ggcatcatgc tgatgatgaa gagccttgcg caggaggtcg
     4381 cgtcccaccg aatccgggtc aacagcatcg cgccgggcgc gattcggacg ccgatcaaca
     4441 cctcggccag gagcacagag aaggcctacg accaactgat gaccttggtg ccctacaaac
     4501 ggatcggcga acccgaggat atcgcgcggg cggtcgtgtg gctcgcgtcc gacgattccg
     4561 attatgtcgt cggcacgacg ctgttcgtcg acggcggcat gaccctctat cccggtttta
     4621 ccgaaggcgg atgatggcgg cgagcaatgg catcgggcgc aatcgaggct gtccgcgggc
     4681 attggcccga atatttgatc gaggccgccg gtctggcgct gttcatgatc tcggccggcg
     4741 ccttcgggac attgctcgaa tacccgcaat cgccggtgca cggcatgctt cccgacccgc
     4801 tcgtccggcg cgccttgatg ggaatagcaa tgggagcaac cgcgatcggt cttatctatt
     4861 cgccgtgggg gcgccagtcc ggggctcatt tcaatcccgc cgtaacatta gccttctcgc
     4921 ggctcggcaa ggtcgcgccc tgggacggcg tgttctatgt gatcgcccaa ttcgtcggag
     4981 ggctggccgg cgttctggcg atttcggccg tgctcggcaa cgcctttcgc gagccgccag
     5041 tcgaattcgt agtgacggtc ccgggctttg ccggcccgct gatcaccttt cttgccgagt
     5101 ttgccatctc gtgcggtttg atgctggccg tcctttacac gagcaatcgc atccacctga
     5161 tgcgctatac tgggctgttc gccggtgctc tgctcgccgg ctacataacg gtagaggcgc
     5221 cgttctccgg gacgagcatg aacccggcgc gaaccgttgc ctcggcagcg ctgagtgggg
     5281 tttgggacgg cgtgtggatc tatttctcgg cgccgctcct cggcatgctg atcgccgtc
//
LOCUS       DATLJT010001027         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2827814,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001027 DATLJT010000000
VERSION     DATLJT010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2827814"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VM910_23635"
     CDS             <1..793
                     /locus_tag="VM910_23635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI65545.1"
                     /translation="AMDPTVLTVKQETAKAAKPGSGFKECANGCPVMIVIPAGKFIMG
                     SPESESDRDASEGPQREVTIAKPFAVSKFEVTFEAWDACVAAAACPRLTDRWGRGEMP
                     VIDASWGDAKQYVGWLSQLTGKEYRLLTEAEWEYAARAGARTRYSWGDGLGTGNADCD
                     GCGSPWDLKQPAPVGSFRPNAWGLYDMHGNVWEWVEDSWHETYDGAPRDGSAWLAGGD
                     PSFRVVRGGSWRNDSELVRAAVRVRRHINVRFDTLGFRVARTLGR"
     gene            962..1636
                     /locus_tag="VM910_23640"
     CDS             962..1636
                     /locus_tag="VM910_23640"
                     /inference="COORDINATES: protein motif:HMM:NF022763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HVI65546.1"
                     /translation="MRTILTIACASVVFAAGAQAQDALRSCRQIKDDGERLKCYDRLD
                     ISSSSRTSRPAESKPGASTAWIINDEKSPLDDSPLVSAALESSDGKAHLLMRCKDRKT
                     ELAVSVRGFIKCGTDIPVTYRIDQAPATETAWHAHSSCYLAVAPSPIPLIRAFADQGK
                     VYFRMFDHHGAAYDALFNLGKVSEIRSRLAEACDWEGTAKATENPASKPPAPAASPSA
                     PKATPK"
     gene            1698..2120
                     /locus_tag="VM910_23645"
     CDS             1698..2120
                     /locus_tag="VM910_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65547.1"
                     /translation="MSDDLKFQLRLTLRDEFAPVARNDPGNPSISPLTNILNRHDAVL
                     KCQFDAFADYVSEAEANGVENYHLYEWTKKTVDDPAKKAKYVKSFTLYVGGEEVYEKD
                     QADALEAELKPLVGGPIVAKMFRYDTDPAHNPQPPRQG"
BASE COUNT          422 a          673 c          685 g          379 t
ORIGIN      
        1 ggcgatggac ccgactgtgc tcacggtcaa gcaggagacg gcaaaggcgg caaagccggg
       61 ctcgggcttc aaggaatgtg cgaacggctg ccccgtgatg atcgtcatcc ccgccggcaa
      121 attcatcatg ggctcgccgg agagcgagtc ggaccgcgat gccagtgaag gcccccagcg
      181 cgaggtgacc atcgcgaagc cattcgcggt ttccaagttc gaggtgacat tcgaagcgtg
      241 ggatgcgtgc gtcgctgcgg cggcatgccc gcgacttaca gaccgctggg gacgcgggga
      301 gatgcctgtg atcgacgcga gttggggcga cgccaagcag tatgtcggct ggctctcgca
      361 gttgactggc aaggagtatc ggcttctgac cgaggccgaa tgggagtacg ccgcgcgcgc
      421 tggcgcccgt acccgctatt cctggggcga tggtcttggc acgggcaatg ccgattgcga
      481 cggctgcggc agtccatggg acctcaagca accggcgccc gtcggatcat tcaggccgaa
      541 cgcgtggggg ctttacgaca tgcacggcaa cgtctgggag tgggttgagg atagttggca
      601 cgagacctac gacggcgcgc cgagggacgg atcggcgtgg ctcgcgggcg gcgacccaag
      661 cttccgggtc gtccgcggcg gttcctggcg caacgacagc gaactcgtcc gcgcggcggt
      721 ccgcgtcagg cgccatatca atgtgcggtt cgacacgctc ggcttccggg tcgctagaac
      781 gctggggcgt tgatccgggg gagccttccc tttcgtccag ccgacggctt gtgatctctc
      841 cgcgcattcc gaattcgcgc ggtacgctgc tcttggagca aggcgtgcga cgcgcccttg
      901 gtgcctgcac ggcctttact ctggtcttgc atgctccgac acgatcggct ctgatgcgcc
      961 catgcgcacc atactgacca tcgcttgcgc cagcgtcgtt tttgccgccg gcgcccaggc
     1021 ccaagacgcc ctcagatctt gccgtcagat caaggacgac ggcgagcgac tgaaatgcta
     1081 cgaccgcctt gatatatcct cctcgagccg aacgagcaga ccggcggaga gcaagccggg
     1141 cgccagtacg gcgtggataa tcaacgatga aaaatccccc ctcgatgaca gccccctggt
     1201 ctcggcggcg ctggagtcga gcgacggcaa ggcccatctg ctgatgcgct gcaaggaccg
     1261 caagaccgag ctcgcggtca gcgtcagggg attcatcaaa tgcggaaccg acatacctgt
     1321 cacctatcgc atcgatcagg ccccagccac ggaaacggca tggcacgcgc attcgagctg
     1381 ctacctggcc gttgctccgt cgccgatccc cctcattcgg gcgttcgcgg atcagggcaa
     1441 agtttatttc cgcatgttcg atcatcacgg cgccgcgtac gacgctttat tcaatctcgg
     1501 caaggtttcc gagattcgtt cccgcctggc ggaggcctgc gattgggagg gcacggccaa
     1561 agcaacggag aatcccgcgt cgaagccgcc cgcgccggcc gcttcgccga gcgcgcccaa
     1621 ggcgacgccg aaataacgct aagcaatcca gcgatgtttc agcgcgcctg cggtgcctcg
     1681 tgcacaggca gagcgccatg agcgatgacc tgaaattcca gttgcggctg acgttacgcg
     1741 acgaattcgc gccagtggcg cgcaacgatc cgggaaatcc gtcgatctcg ccgctgacga
     1801 atatcttgaa ccggcacgat gccgtcctga aatgccagtt cgacgcgttt gcagattatg
     1861 tcagcgaggc cgaggctaac ggcgtcgaaa attaccatct ttatgaatgg accaaaaaaa
     1921 ccgtcgacga ccctgccaag aaggcaaaat acgtcaaatc gttcacgctc tatgtcgggg
     1981 gagaggaggt ttacgaaaag gatcaagcgg acgcgctcga ggccgagctc aagccgctgg
     2041 tcggaggtcc gatcgtcgcg aagatgttca gatacgacac cgatccggcc cacaaccccc
     2101 agcctccgcg ccagggctag tgtcccgatt ccgaagttcg catcattgtg ccgcagcct
//
LOCUS       DATLJT010001028         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1909371,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001028 DATLJT010000000
VERSION     DATLJT010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909371"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..913
                     /locus_tag="VM910_23650"
     CDS             23..913
                     /locus_tag="VM910_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVI65548.1"
                     /translation="MATAQVKQHDYHLVDPSPWPIVGSVSVFIMAVGAIGWMKHLYSA
                     AWMVFALGMIGVLYTMASWWSDVIKEAQYKGDHTRVVQISHRYGMILFIASEVMFFVA
                     WFWAFFNSALFPADAVHAARDAIFGCGEGTAIGACSVPGTWPPKGIEAFDPWHLPLLN
                     TLLLLTSGTTVTWAHHALLGNDRQGLKYGLILTIILGAAFTCVQAYEYSHALFHFSGN
                     VYGATFFMATGFHGFHVLIGTIFLIVCLGRAYAGHFTPKQHLGFEFAAWYWHFVDVVW
                     LFLFVCIYVWGTGGGRVGVE"
     gene            complement(990..1397)
                     /locus_tag="VM910_23655"
     CDS             complement(990..1397)
                     /locus_tag="VM910_23655"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HVI65549.1"
                     /translation="MLEEFFSMREPYAVVESWAESFNAGDVAAIAALYAPDATIWGTL
                     GQYLTTQPAEIRSYFGEAFRAGLKVKLQPFVIHTIGGDGVIVAGHYEFSRIADSQTAA
                     YPARYSFVLVRTADGGWLITHHHSSLLPKPLDT"
     gene            1426..1827
                     /locus_tag="VM910_23660"
     CDS             1426..1827
                     /locus_tag="VM910_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HVI65550.1"
                     /translation="MTNESASPAAPQPTVFESALRGLACRCPRCGRGKLYAGFLTLRA
                     KCDVCGLDYAFIDSGDGPAVFVIMIAGAIVVACALIVEVKYQPPFWVHAALWLPLTLA
                     VTLWPLRAIKSLLIALQFHHKAAEGRLIERK"
     gene            1824..2591
                     /locus_tag="VM910_23665"
     CDS             1824..2591
                     /locus_tag="VM910_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HVI65551.1"
                     /translation="MTGRRFGFGLFTLVMIVAFVGLGAWQLQRRVEKHALIAALTERL
                     AAAPIPLPPSSQWRAHAATGDEFRRVAFTATYQRLPDAMVYSAGSAVREDVSGPGTWA
                     FLAARLPSGETIVINAGFVENTMQDRGVEDRAVAKLVTGEPVAMTGYLRFPEQAGPLT
                     PADNPTKRLWFVRDHLAMAHALGWGEVAPFYIDLETPAPASGIPKPGPLAVHLRDDHM
                     QYAITWFALAGAVAIAFLVWWRGRRNTQASPGAASEI"
     gene            complement(2593..>3204)
                     /locus_tag="VM910_23670"
     CDS             complement(2593..>3204)
                     /locus_tag="VM910_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HVI65552.1"
                     /translation="SSPPFDLHCPIGSLPLAFQTRLDTVPAAPYLPPPAAERVQAWER
                     RLGARDRLRVGLVWSGNRQHKNDKIRSLPFHTLSPILEVDATFVSLQKDLRPDDQPFL
                     KARSDVIDLTADLTDFTETAALISCLDLVITVDTSVANLAGALGRRTWILLPYTPDFR
                     WLLDRDDSPWYPTMRLFRQDESRDYGRVIERVRAALAELVATR"
BASE COUNT          529 a         1015 c         1029 g          631 t
ORIGIN      
        1 accgctaacg gagagaccga taatggctac ggcgcaagtc aagcagcacg actaccacct
       61 cgtcgatccg agcccgtggc cgatcgtcgg ctccgtgtcg gtcttcatca tggcggtcgg
      121 cgcgatcggc tggatgaagc acctgtattc ggccgcctgg atggtgttcg cgctcggcat
      181 gatcggcgtg ctctacacca tggcgagctg gtggagcgac gtcatcaagg aggcccagta
      241 caagggcgac cacacccgcg tcgtgcagat cagccaccgc tacggtatga tattgttcat
      301 cgcctccgag gtgatgttct tcgtcgcctg gttctgggct ttcttcaatt ccgcgctgtt
      361 ccctgccgat gcagtccacg ccgcgcgcga cgcgatcttc ggctgcggcg agggcaccgc
      421 catcggcgcc tgctctgtcc ccggcacctg gccgcccaag ggcatcgagg cctttgatcc
      481 ctggcacctg ccgctgctta acacgctgtt gttgctcacc tccggcacca cagtgacttg
      541 ggcgcatcac gcgctgctcg ggaacgaccg tcagggcctg aaatacggac tgatcctgac
      601 tatcatcctc ggtgcggcct tcacctgcgt gcaagcttat gagtacagcc acgccctgtt
      661 ccatttttcc ggtaacgtct atggcgcgac gttcttcatg gcgaccggtt tccacggctt
      721 ccatgtgctg atcggcacca tcttcctgat cgtgtgcctg ggccgcgcct atgccgggca
      781 cttcacgccg aagcagcatc tcggcttcga gttcgccgcc tggtactggc acttcgtcga
      841 cgtggtctgg ttgttcctgt tcgtctgcat ttatgtctgg ggcaccggcg gcggcagggt
      901 cggcgtcgag tagttatgca tcatgggcgg gaaaaagggc ggccgcaggc cgcccttttt
      961 gtttgccgtc tctcaaacgg agaggtttct taagtgtcga gcggcttggg caacagcgac
     1021 gaatggtggt gcgtgatcag ccagccgccg tcggcagttc tcaccagcac aaagctgtat
     1081 cgcgccggat aggcagccgt ctggctgtcg gcgatgcgcg agaactcgta gtggccggca
     1141 acgatgacgc cgtctccgcc gatcgtgtgg atcacgaagg gctggagctt caccttcagg
     1201 cccgcgcgga acgcttcgcc gaaatagctc ctgatctcgg caggctgggt cgtgagatat
     1261 tggcccagcg tgccccagat cgtggcgtcc ggcgcgtaga gcgcggcgat agccgcgaca
     1321 tcgcccgcat tgaaactttc cgcccagctt tccaccacgg catagggctc gcgcatcgag
     1381 aaaaactcct ccaacaagcc aagctccaac gtgatacact gtcttatgac gaatgaatcc
     1441 gccagtcctg cggcgccgca gccgaccgtt ttcgagagcg cgctgcgcgg gcttgcctgc
     1501 agatgtcccc gctgcggacg gggcaagctc tatgcgggct ttctgacttt gagggccaaa
     1561 tgcgacgtct gcgggctcga ctatgccttc atcgattccg gcgacggccc ggccgtcttc
     1621 gtcatcatga tcgcgggtgc gatcgtcgtc gcctgcgccc tgatcgtcga ggtcaaatac
     1681 cagcccccgt tctgggtgca tgcggcgctg tggttgccgc tgacgctcgc ggtgacgttg
     1741 tggccgttgc gcgcgatcaa gtcgcttttg atcgcattgc agttccatca caaggccgct
     1801 gagggccgcc ttatcgagcg caaatgaccg gacgccgctt cggcttcggc ctcttcacgc
     1861 ttgtgatgat cgtggcgttc gtcggcctcg gcgcctggca actgcagcgt cgcgtcgaga
     1921 agcacgcgtt gattgctgcc ctcacagaac gcctcgcagc cgcgccaata cccctgccgc
     1981 catcatcgca gtggcgcgcg cacgcggcaa cgggcgacga attccgccgc gtcgccttta
     2041 cggccaccta tcaacgcctg cccgatgcga tggtctattc cgcaggctcc gccgtgcgcg
     2101 aggatgtttc agggcccggc acctgggcgt tcctggccgc ccgcttgccg tccggcgaaa
     2161 cgatcgtgat caatgccggc tttgtcgaaa acacgatgca ggaccgcggc gtggaggatc
     2221 gcgcggtcgc aaaactcgtc acgggcgagc cggtcgcaat gaccggttat ctgcgctttc
     2281 cggagcaagc aggcccgctc acgcccgcgg acaatccgac caagcgcctg tggttcgtgc
     2341 gcgatcatct agcgatggcg catgccttgg gttggggcga ggtcgcgccc ttctatatcg
     2401 atctcgagac gccggcgccc gcaagcggca ttccgaagcc ggggccgctc gcggtgcatt
     2461 tgcgcgacga tcacatgcaa tatgcgatca cctggtttgc gctcgccggc gcggtagcga
     2521 ttgcgtttct ggtgtggtgg cgaggcaggc ggaatacgca agcctcgcct ggagcggcaa
     2581 gcgagatttg aattacctgg tggcaaccag ttcggccagt gccgcacgga cgcgttcaat
     2641 gacgcggccg tagtcccggc tctcgtcctg cctgaacagg cgcatcgtcg ggtaccaggg
     2701 gctgtcgtcg cggtcgagca gccagcggaa gtccggcgtg tagggcagca ggatccaggt
     2761 ccgccgtccg agcgcgccgg cgagattggc gacgctcgtg tccacggtga tcaccagatc
     2821 gaggcacgag atcaatgcgg ccgtctcggt gaaatcggtg agatcggcgg tgaggtcgat
     2881 gacatcggaa cgcgccttca ggaacggctg gtcgtcgggc cgcaaatcct tctgcaggct
     2941 gacgaaggtc gcatcgacct cgaggatcgg cgacaacgta tggaacggca gcgagcggat
     3001 tttgtcgttc ttgtgctgcc ggttgcccga ccacacgaga cctacgcgca atctgtcgcg
     3061 cgcgccgagg cgtcgctccc aggcttgcac gcgctccgcc gcgggcggcg gcagataggg
     3121 cgcggcggga acagtgtcga gccgcgtttg gaaggcgagc ggcaggctgc cgatcgggca
     3181 atgcagatcg aaaggtggcg acga
//
LOCUS       DATLJT010001029         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3517459,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001029 DATLJT010000000
VERSION     DATLJT010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517459"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(556..1365)
                     /locus_tag="VM910_23675"
     CDS             complement(556..1365)
                     /locus_tag="VM910_23675"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65553.1"
                     /translation="MRVIGVLVAGTADDPEHQARVEAFRQGLERLGWTDGRNVRFDIR
                     WATTNADALRKHAAELAALAPDVIVSAAGTTTTAPLLEATRDLPIVFVILIDPVGAGF
                     VASLARPGGNATGFLMFEYGLSGKWLELLRQIAPSVTRVAVLRDPTIASGIGQFGAIQ
                     SAAPSLGLEATPFNVRDAGEIEHDIASFARSANGGMIVTTTPKTLIHRDLIIALAARH
                     RLPAVYSTRPPADLPVQAPTKYELLINLKTAKALGLELPAALVARADEVIE"
     gene            complement(1886..>2654)
                     /locus_tag="VM910_23680"
     CDS             complement(1886..>2654)
                     /locus_tag="VM910_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI65554.1"
                     /translation="AWLRGEPRVCSMVPIPHEADEDARRCVRERSELVSERVGLVNRI
                     GAVLATLGTDDYNPLLQSRRRRLIELRTGLGDPIPPNALAKIGRLLTRLELVLTQIAE
                     LELERDAVARADAPDQAGRMIQQLISLRGIGVQSATVLVREAFVRRFANGKALGSYAG
                     LTSTPYSSGGIDREQGIGKAGNRRLRTVMVELAWLWQRYQPGTAQVVWFRERVGSTGR
                     RLRKVMVVALARKLLIALWRFVIDGLMPEGAVTKPSI"
BASE COUNT          519 a          861 c          803 g          471 t
ORIGIN      
        1 gtccttgccc tcggaccatc tcagctcctt catccggttt tggaaatgag taggcggagt
       61 tgaggcgccg aaaccaagat acccaacctt cggaagccgc tcagtttgcg caggagccaa
      121 gctcgggctc ccgaagagca taacagcggc aacaaatgga gccgacaagg agcagatccg
      181 atctagccag agagcccgaa gcctcatctc atcctccctt ggcttcttag atgccgaagg
      241 aactcatcca ctcgatcgcc tgaggtggcg tcgaccaggc tgatagctgc gcaagcgccg
      301 tgcccggcca caccgaagcc gcacgcgcaa tccggcgtcg catggggcgc cttgctaagg
      361 acacgcataa tccgatgctc ggaccctaac actttgcgag tggaaggcca tcaatgatcg
      421 accgggcgcg tccatacgcc acgtctgttg cgggtcatta gcagacattc gtcgagcccg
      481 agcggatgcc cgctctgccc ccggaagcgg acatcagagg cgcgcgccgc cacgtctgct
      541 ttgggccaga agcggtcatt cgatcacttc atcggcgcga gcgacgagcg ccgcgggcag
      601 ctccaggcca agggcttttg ccgtcttcag gttaatcagc aactcatatt tggtgggcgc
      661 ctgcaccggc aggtccgccg ggggccgggt gctgtagacc gctggcaaac ggtgccgggc
      721 cgccagcgcg ataatcagat cacgatgaat caaggttttc ggcgtcgtcg taacgatcat
      781 gccgccgttt gcggaacgtg caaaggatgc gatgtcgtgc tcgatctcgc cggcgtcgcg
      841 cacgttgaac ggggttgctt ccaacccgag ggatggcgcg gcagactgga tggcgccgaa
      901 ctgtccgatc ccggaggcta tagtgggatc gcgaagcacg gcaacgcgcg taacgcttgg
      961 tgcgatctgc ctgagcagtt ccagccactt tccgctcaga ccgtattcga acatgagaaa
     1021 cccggtggcg ttgccgcccg gccgcgccaa gcttgcgacg aaaccagcac cgacagggtc
     1081 gatgaggatc acgaacacga tcggcaggtc gcgggtcgct tccagcaatg gcgccgtcgt
     1141 tgtcgtgccg gctgcggaca cgatgacatc cggcgcgagc gcagccagct cggccgcgtg
     1201 cttgcgcagg gcgtcagcat tggtcgtagc ccaacggatg tcgaaccgta cgttgcggcc
     1261 gtcggtccaa cccagccgct ccaaaccttg ccggaatgcc tcgacacggg cctgatgttc
     1321 tggatcgtca gcggttccgg ctaccagcac gccgatgacc cgcatccgat cgggctgctg
     1381 cgcatgcgcc acgagcggcc atgccgcacc gccgcccacg agcgtgatga actcccgccg
     1441 cctcataggc tccccctatg gccgacgaga acaataccac attgctgaac caatgcctgg
     1501 ctttgcattg cagaaatccg gctgccgacg tcggtttggg gtcatgtgtg gacggcgccc
     1561 gcattgcaag aggaatctga cgttcggctc gcggtcgggt gcaagtcatg tgtccggcct
     1621 cacatgatga ttcctctgtc aatggctttc tgccgacggc gatccgaggt gagctctctt
     1681 gctgcagggc aaggcttccc tgctaacctc acatccggcg ggatggtgat cccgaccact
     1741 gtcccgcatt cgggacggga gagcttcgaa ggaacgccac cactctgtga agacggcgcc
     1801 gaagcaccac gccggacaac ggcgacgttc cctcggatca ccggactgca aggccgttgc
     1861 ggcgctgccc ggttttcgga gacggttaga tggagggctt cgtgaccgca ccctcaggca
     1921 tcagaccgtc gatgacgaat cgccataagg caattaggag tttacgcgcg agcgccacca
     1981 ccatgacctt acgaaggcgg cggccagtcg agccgacgcg ctcgcggaac cagaccacct
     2041 gcgcggtgcc cggctggtag cgttgccaaa gccaggccaa ttcgaccatc accgttctga
     2101 gccgacggtt gcctgctttc ccgataccct gctcacgatc gatgcccccg ctgctatacg
     2161 gagtcgaggt gaggccggca tatgacccaa gcgccttgcc attagcaaag cgtcggacaa
     2221 aggcttctcg caccagcacg gttgcgctct gcactccgat gccgcgcagg ctgattagct
     2281 gctggatcat cctaccggcc tggtccggag catcggctct agcgacggca tcacgttcaa
     2341 gttctaactc cgcgatctgc gtaagtacga gttctagcct cgtaagcaaa cgcccgatct
     2401 tagccagtgc atttggcggt atcggatcgc caaggcccgt ccgcagctca atcagacggc
     2461 gacgtcggct ttgaagaagg ggattgtagt cgtcagtgcc cagcgtcgcc agaacggcgc
     2521 cgatccgatt cacgaggccg acacgctcgg agaccaactc actccgctcc cggacacacc
     2581 gccgcgcatc ctcatcggct tcgtgcggga tcggcaccat cgaacagacg cgcggctcgc
     2641 cgcgaagcca ggca
//
LOCUS       DATLJT010001030         1560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3287932,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001030 DATLJT010000000
VERSION     DATLJT010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3287932"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VM910_23685"
     CDS             complement(<1..641)
                     /locus_tag="VM910_23685"
                     /inference="COORDINATES: protein motif:HMM:NF018498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HVI65555.1"
                     /translation="MSASRPIIAVLATMDTKSNEARFIADALTRAGATPWTVDLSMKS
                     HTLSAADVSGAQVAEAVGVSWQALDERSRQEAGAVMVQGGTRILLERYSKGEIAGAIG
                     VGGANGTNLVCSILRALPYLLPKIMISTVAGTAAVQWYVAESDIAMYPSIGDVSLNRI
                     TRAVMENAANAVALAAANWVARREAKTVHAPLVAVSSFGGTAACVERVSQRLQA"
     gene            1298..>1560
                     /locus_tag="VM910_23690"
     CDS             1298..>1560
                     /locus_tag="VM910_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65556.1"
                     /translation="MVDRFLREVQRMHQLRRDISEMASAEADELIRGLGDDAAEDWVF
                     AARDAQLPPPDLSWCWLFLGGRGAGKSHSMSAAVHTAVRAGLKR"
BASE COUNT          317 a          474 c          474 g          295 t
ORIGIN      
        1 gcttgcagcc gctgactgac ccgctcaacg cacgccgccg ttcctccgaa cgacgagacc
       61 gcgacgaggg gcgcatgaac ggtcttcgcc tcccgtcttg ccacccagtt cgcggctgca
      121 agtgccaccg cgttcgcggc attctccatg accgccctgg taatgcggtt gagcgagacg
      181 tcgccaatcg aagggtacat ggcgatgtcg ctctctgcga cataccattg cacggcggcc
      241 gtgccggcca ccgtgctgat catgatcttc ggcaagaggt atggcaatgc gcgcaggatc
      301 gagcacacga gattggtccc gttcgcgccg ccgacgccga tggcgcctgc gatttctccc
      361 ttggagtacc tctcaagcaa aatcctggtg ccaccttgga ccatcacggc tcctgcttct
      421 tggcgtgagc gttcatcgag cgcctgccaa gacacgccca ctgcctcagc aacctgcgca
      481 ccggaaacgt ccgcagcaga caaggtgtgc gatttcattg acaggtcgac ggtccagggc
      541 gtcgcacccg cacgtgtcag cgcgtcggca atgaagcggg cttcgttgct tttcgtgtcc
      601 atcgttgcca ggactgcgat gatcggtctt gatgcgctca agaacccccc acgagccgct
      661 ctcctatcaa ccatccggtg acactagaca gattccaccg ctagaggaag ttgaccgact
      721 tcatggaagg aagtcgctag cctactgagc cttctttcat ttgatcgtct cgtcagtcag
      781 ggccggcaac ctaaggcagt cgccgattct gactgggcct gaacgatttg gtgggcggtg
      841 taaggatcga acctacgacc tcgctgagcc tgcggtagac cgaccatttg ggcctgtcgg
      901 ttgccagtgg tcactcctaa gaaggcgggc cgcccgcagc aagacgggaa aagatgtccg
      961 ccgggacgct ggcgttggtg cgatcgctct tgaccttgcg atgctcgaca gagcaaaacg
     1021 cgcttcacac attgatccac cagcgtcatc ttacgccatc atgcggaggg cagcccctac
     1081 cgcggagaga acagtgggcc cggcaaggat gatcgcggag agcttgacac cgaaacccgg
     1141 aattagagaa cacaatcggc acgaagcgga catttccgtc gcgtcggtca acgccacgtg
     1201 ttgctgacga ccgagaaagt tcgcgcgctc gtcgataata taagaggcaa gaaagatcgc
     1261 gacatcgaag gcgaagtcgt gaaaaaggac gacgacgatg gttgatcgct ttttgcgtga
     1321 agttcagcgc atgcaccagc tgcgccggga catcagcgag atggcatcgg cggaagccga
     1381 tgagttgata cgaggactgg gcgatgatgc ggccgaggac tgggtctttg ccgcgcgcga
     1441 tgcacaactg ccgccgcccg acctgtcatg gtgctggctg tttcttggag gccgcggcgc
     1501 cggcaaatcg cactcgatgt cggcggcggt gcataccgcg gtgcgcgctg gcctaaagcg
//
LOCUS       DATLJT010001031         1887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4436781,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001031 DATLJT010000000
VERSION     DATLJT010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4436781"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(26..143)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            378..824
                     /locus_tag="VM910_23695"
     CDS             378..824
                     /locus_tag="VM910_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65557.1"
                     /translation="MYRHILIPTDGSELSRRAVQHGLALAKAVGAKVTALTVEGSFDV
                     YTVPASRVHEMSGAFAEHAARAKAHAEKILSGVAEAARLAGVVCETVQIEEDHPYEAI
                     IETANQRGCDLIVMASHGRSGIAAIVLGSVTTKVLTHTKIPVLVCH"
     gene            complement(912..>1887)
                     /locus_tag="VM910_23700"
     CDS             complement(912..>1887)
                     /locus_tag="VM910_23700"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI65558.1"
                     /translation="ALQARAESMDALYAKAKLEKTVVLYGAGPTGSHDRWIKDFEQRF
                     PGVTVTFTGGLSTDLNQRIERQLASGTVETDLAILQTIEDFGKWKKAGALLPFKPEGS
                     DAIDPAYKDEDGAFTAVSINTIVYAYNTNLVPSADAPTSALDFLKSRFQGGLITTDPT
                     QDDAGLAVFTMIVGKYGWDYMDKYMAQKPAFVTSGHAAVSNAIAAGEKLATFDSTSTT
                     PRLKQAGKPIEPVFSRADPTPVFLVGAAIFKHAPHPNAAKLYLTWYLAKEQQSRSGTF
                     SPRSDVAPPRGLEPLSSYNIDRGYRALVSDEARLTQLRARLRSYIAQR"
BASE COUNT          335 a          544 c          650 g          358 t
ORIGIN      
        1 gaaagagcga gatgcgcggg gaagctactg ttgtcatgtc ctgtcccttc gccggcatga
       61 cccggatcag gttcaaaggg tccccgcggt atcgatcggg cgggtctctc agctccttga
      121 ccggagctcc cctcggaaca cccgttaatg taggcccgcc ggggccgctg tcaacgcgcg
      181 cggcgcggat cggatgcgat ctgccgtttg caggcgaacg cggcttaggc ccgagtcgct
      241 tgccattggc cggcgaaagc cgaggcgaaa acgccgtttg aacgatgtcg gttgcaaccg
      301 tattgaagta aataaagcac agctgcgaac gcattgttcc agaggtggct gcgcgaggca
      361 tgacagggag ggacggcatg tatcggcata ttttgatccc aaccgacggt tccgagcttt
      421 cccgcagggc ggtgcagcat gggttggcgc tcgccaaagc cgttggagca aaagtcaccg
      481 cattgacggt ggagggatcg ttcgatgtct acacggtccc tgcttcgagg gtccatgaaa
      541 tgtcaggagc ttttgccgag catgccgcgc gcgccaaagc gcacgcggaa aagatattga
      601 gtggcgtcgc cgaagcagcg cgtttggccg gtgtcgtctg cgaaaccgtg caaatcgaag
      661 aggatcatcc ttacgaagcg atcatcgaga ccgccaacca aagaggctgc gatctcatcg
      721 tcatggcctc gcatggcagg agcgggatcg cggcgatcgt gcttggcagt gtgacgacga
      781 aggtgctgac gcatacgaaa attccggtgc tggtgtgcca ttgatgcgaa ggagccgcgg
      841 tcgttcatgc cgcgaaggcc ttaagcgttg gagccggcgg tggcgctcat cgccggcgct
      901 ggctcggtga ctcaccgctg ggcgatgtaa ctgcgcaacc gcgcgcgcaa ttgcgtcagc
      961 cgcgcctcgt ccgacaccaa ggcccgatag ccgcggtcga tgttgtagga ggacagcggc
     1021 tcgaggcccc gaggcggcgc aacgtcgctg cgcggcgaga acgtgccgct gcggctctgc
     1081 tgctccttcg ccaggtacca ggtgagatag agcttggccg cgttggggtg cggcgcgtgc
     1141 ttgaagatcg ccgcgccgac caggaacacc ggcgtgggat cggcgcgcga gaacaccggc
     1201 tcgatcggct tgcccgcctg cttgagccgc ggcgtggtcg aggtggaatc gaaggtcgcg
     1261 agcttctcgc cggcagcaat ggcgttggac acggcggcat gcccgctggt gacgaaggcg
     1321 ggcttctgcg ccatgtactt gtccatgtag tcccagccgt atttccccac gatcatggtg
     1381 aagaccgcga ggccggcgtc gtcctgcgtg ggatcggtgg tgatgaggcc gccttgaaac
     1441 cggcttttga ggaagtcgag cgccgatgtc ggtgcgtcgg cggaaggaac gagattggtg
     1501 ttgtaggcgt agacgatggt gttgatgctg accgccgtga aagcgccgtc ctcgtccttg
     1561 taggcaggat cgatcgcgtc ggagccttcg ggcttgaagg gcagcagcgc gcccgccttc
     1621 ttccacttgc cgaaatcctc gatggtctgg agaatcgcga ggtcggtctc cactgtcccg
     1681 ctcgcgagtt gccgctcgat ccgttgattg aggtcggtgc tcaagccgcc ggtgaaggtg
     1741 accgtgacgc cgggaaagcg ttgctcgaaa tccttgatcc agcggtcatg ggagccggtc
     1801 ggtcccgcgc cgtagagcac gacggtcttt tccagcttgg ccttcgcata gagcgcgtcc
     1861 atgctctcgg cgcgcgcctg caacgcg
//
LOCUS       DATLJT010001032         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_71004,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001032 DATLJT010000000
VERSION     DATLJT010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71004"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(792..1106)
                     /locus_tag="VM910_23705"
     CDS             complement(792..1106)
                     /locus_tag="VM910_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65559.1"
                     /translation="MTQEVEELRRLLRESASRPVCPESSSGLRASRGFIDDVVVPVVG
                     DLLKETRSANARLTDQVQHLKRRVELLDIRLRAPNESRALKFRIFNLEKEIALLRRAA
                     KK"
     gene            complement(1179..1505)
                     /locus_tag="VM910_23710"
     CDS             complement(1179..1505)
                     /locus_tag="VM910_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65560.1"
                     /translation="MLALSDQGLAHLVIAASAVAPERRAAWLHDLARKLEQAAPSPAA
                     VRQRRHRHRVRSGRAVFRVELGVLDIELYLVGAGLLRPGDVDRSAIEAALARLLERLI
                     AAGGYA"
     gene            complement(1926..2048)
                     /locus_tag="VM910_23715"
     CDS             complement(1926..2048)
                     /locus_tag="VM910_23715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65561.1"
                     /translation="MPKTVFTARLETLAVALDATTTGEGEVIQEMTAVGGALIP"
     gene            2629..2889
                     /locus_tag="VM910_23720"
     CDS             2629..2889
                     /locus_tag="VM910_23720"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HVI65562.1"
                     /translation="MSANCATWTSERIELLKRCLHAGLSCGQTAREIGVTRNAVIVIG
                     RQLEQRRAARLARPKTPRTWRLQRFAALPCATMNFSGSMSRP"
BASE COUNT          649 a         1040 c         1023 g          693 t
ORIGIN      
        1 agcgccggaa tgctgacagc aaccttccgc cgcttgggcg gcgctgctcg cctgcccttc
       61 ttggacttct tgatattcaa tttggctttc gatttcgcct tcgacttcgc tttttttggc
      121 atggcgggcc acgttcaaag ttgattgagt atatctgtcc aaagacatgc gtcgccagga
      181 cgggcccggc catgaggcgt cgcaaggata ggtatcagcc gcgcgccgct tcggcaagcc
      241 agccgaagac gttgaagacg tacgcttgca gtgcaattgg acgcgacagg ttgacgagcg
      301 aggagcgatt atcagacatt gtcgaaatct tggcgcaggg tctgctttgg cttgatgtcc
      361 cgagggacta tcatggctcc attcgatccc atacttccgc cgtttcgtca ccgaaggcgg
      421 attgatctcc ttcgggattg atcaggccgc gttgtatcgg cttgccgcct cctacgtcga
      481 ccgcatccag aaggcgagaa accagccgac ctcccggctt ggggggtcac caaagggtcg
      541 cccccacgtc cattgagccc cccgaaatga gcggcctagc gacaaacgag attgctggtt
      601 tcctgatcgt tgcgtttttg ctggctctgc tactcttttt tcttctccgc tgaaaagcgc
      661 gaagaatcgg gaagcctaga accgccgttc ccgatgttat ggactaacac cggaaacggc
      721 gggccgcgcc tgcgcggtgc atacagccag ctaccaccgc gccacctgca aataggggaa
      781 atggcatctc gttacttttt cgctgcacgg cgtagtaggg cgatttcctt ctctaaattg
      841 aatattcgga atttcagcgc gcggctctca ttcggcgcgc gcaggcgaat atcaagcagc
      901 tccacgcggc gctttaagtg ctgcacctga tcagtcagcc tagcgtttgc actgcgcgtt
      961 tctttcaaca agtcgccaac caccggaacg acgacgtcgt cgataaagcc gcgactcgct
     1021 cgcaagccac tcgaggactc ggggcagacg ggcctgctgg cgctctcccg aagcagtcgt
     1081 cgcaattctt ccacttcttg cgtcattgtg aacctcatct cgcttaggct cgtgcaagtg
     1141 ctagaaaaaa ttatagcgcg tttcgtggag gcgcgacgtt atgcgtaacc tccagctgca
     1201 atgagccttt cgagaagtcg cgctagcgcg gcctcgatcg cgcttcgatc gacgtcacca
     1261 ggacgaagca agccggcgcc gacgagataa agctcgatgt cgagaacgcc gagctccacc
     1321 ctgaacacag ctcgaccgga acgaacacgg tggcgatgtc gacgctggcg caccgcggcg
     1381 ggggatggcg cagcctgctc gagcttccgc gctaggtcat gcagccatgc agcccgtcgt
     1441 tccggcgcaa ccgcggaagc ggcaatgacg agatgcgcca ggccctgatc actcagcgca
     1501 agcacggcgc ttgacctttt cggtcgtcag caacaggttg tggcgaacat tcatggaagg
     1561 ttattttgct taagttgcga actcaccgcg cgttgggcat cacggcggcg accgcccaaa
     1621 acacttgcag gcccccccgc tggagaagga ttttctgcgg aattctttgt attgggcttc
     1681 aggtcgagca tcgcgtggcc ccgccagtta gccccgttgg tcgggcttag gatcaacgcg
     1741 gtgaggcggc tggtcacggc cgaaggtgcg gcggccgtgg cggcatcgcg cggttcagcg
     1801 ccgtgctata gttgaggcat tgccgaaatg attcggtcga tttgacacga gaggaatttg
     1861 acgatgctgc gatgtccaag atgctcaaga tgcatttcag tcggtctggt ctgcgcctgc
     1921 ctgtttcagg ggatcaaggc gccgcctact gctgtcatct cctggatcac ctcgccctcg
     1981 ccggtggtcg tggcgtcgag cgccaccgcc agcgtctcga gcctcgccgt aaatacggtc
     2041 ttgggcaaga cgtacgacgt tgccccgatc cggaagccgc tacacggcga gcccgacgag
     2101 actcgccact aggcggggat ggagttggat cgtcgtcgcg gagtgcgatt gacgacgcca
     2161 ggcgcgagcg cactttctta gtcattcctt ggtccaaccg tgctggcgac ccaccttgct
     2221 gctgggccgc aagttgctca cgatcgaacc aggacgagga gctctcagct gcctggctca
     2281 ggcgaggaga gggaacgccc tccgcgcctc agaccacccg cctacctttc ctcttctcag
     2341 tcctcttcac agcgacgggt ggctgctgaa tgggaattta gggccagccg tctcggctgg
     2401 tcaacaccta aagaattccc gagcttgggc cgaaggcgag cccaatcgcg gctcgcgtgc
     2461 tccttgcccg ggcctagccc gcgcgcatct tccgttgccg aaagcaagga gggatggggc
     2521 tttctgcgcg ctcccggcat tatcgaattg ttaaccatag ctttgcaaca ctgcaacatt
     2581 ttcagaacta ctcattccag ccatcatccg cggagcaagg cgatcaacat gtccgcgaat
     2641 tgtgcgacct ggacttcgga gcgcatcgaa ctgctgaagc gttgcctcca tgccggcctt
     2701 tcgtgcggcc aaaccgcccg cgagatcgga gtcactcgca acgccgtcat tgtgatcggc
     2761 aggcagctgg agcaaaggcg tgccgcaagg cttgcgcgcc ccaagactcc aaggacttgg
     2821 cgcttgcaga ggttcgccgc gttgccgtgc gcgaccatga acttctcggg gtcgatgtcc
     2881 aggccgtagc ggaccatggc gccgggatcg ctccacacac gacggcaggc gagccatgag
     2941 gcgggacgta ttcggacaac gtaggcgtca cgtcgcgcgg ctggtgcttg agcccgagcc
     3001 ggtggaaggc gcggccgagg cgatccgtta acgtgccata tttggcttta tgcgagagca
     3061 tatggacggt agcgcgccga aagtactcca ccgtctcgga aaggtaagga gcaccaaaga
     3121 ggcgcaggaa tccaaccgaa aggcggcagt cgtcgcgggg agggagtgcc ttagatcact
     3181 gacgttgcgg cggcgcgcgc tccaacgcgg cccgggttct ggtcccgtct gtggtccgcg
     3241 ccccgcgcca tatgtggctc atggaccaaa acgctgtctg ttgtgccgca gtcggtgcac
     3301 atgaacgcca caagaccggg cccgcctccg aatggagcga tctccgctac cctcgacatt
     3361 ggatgccggc accgcttgca ggctctctgc gccaccttca ttgtc
//
LOCUS       DATLJT010001033         4142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_71056,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001033 DATLJT010000000
VERSION     DATLJT010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71056"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VM910_23725"
     CDS             <1..856
                     /locus_tag="VM910_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI65563.1"
                     /translation="VDLRPENIGRRVPVDLGVVGDVRATLAALLPLLKGKRDGKHLSQ
                     AQQHYAKSRKELDDLAKGTPGKRLIHPQQIAKAISDQAATDAIFTCDVGLPTVWAARY
                     LAMNGKRRLLGSFWHGSMANAMAQAIGAQAAFPGRQVISLSGDGGFSMLMGDFISLTQ
                     LKLPVKVVVFNNGTLGFVELEQKSTGFLDFGVDFENPNFAAMAKAMGIYGVRLEDPAD
                     VERGIAAALAHDGPVLIDAVVNRTELAMPPSITLEMAKGFTLFMVKAVMSGRAEEVID
                     LAKTNLWR"
     gene            1107..2242
                     /locus_tag="VM910_23730"
                     /pseudo
     CDS             1107..2242
                     /locus_tag="VM910_23730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2532..3956
                     /gene="fumC"
                     /locus_tag="VM910_23735"
     CDS             2532..3956
                     /gene="fumC"
                     /locus_tag="VM910_23735"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVI65564.1"
                     /translation="MGQTRKESDSLGVVEVPADKLWGAQTQRSLEHFSIGRELIPPEM
                     ITAYAILKKAAAAANHAGKRLDDQRYKLIVQVCDEILAGKHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCSQLAGTPLGSKTPVHPNDHVNMAQSSNDTFPSAMYIAAAVNVKRSL
                     LPAVAALRDAISAKAQAWDDIVKIGRTHMQDATPLTLGQEWSGYAGMLSDDLERIEAA
                     LQGVYQLALGGTAVGTGINSAPGFAEAAAAEIAKLTGLPFVTAPNKFTVQGAHDALVQ
                     LSGTLRTLAVSLYKIANDIRLMSCGPRAGFAELSIPENEPGSSIMPGKVNPTQCEALA
                     MLAVQVMGNDVAVGFGGASGYLEMNVYKPLMIFNAIHSIILMSDGCVNFRKFLVEGTK
                     PNLKKINEYVERSLMLVTALSPVIGYDKASKVAHHAMDNDLTLKEAALQLGFVTEAEF
                     DRIVDPKKMVKPYVASEASTKGPH"
BASE COUNT          774 a         1298 c         1289 g          781 t
ORIGIN      
        1 ggtcgatctg cgaccggaga acatcggtcg ccgcgtgcct gtcgatctcg gcgtcgtcgg
       61 tgatgtccgg gcaacgctgg cggcgctgct gccgctgctc aagggaaagc gcgacggcaa
      121 gcacctcagc caagcgcagc agcattacgc gaagtcacgc aaggaactcg acgatctcgc
      181 caaagggacg cccggcaagc ggctgatcca tccgcagcaa atcgccaagg caatcagcga
      241 ccaggcggca acggatgcga tcttcacatg cgatgtcggc ctgccgacgg tgtgggccgc
      301 gcgctacctg gcgatgaacg gcaagcgccg gctgctcggg tcgttctggc acggctccat
      361 ggccaatgcc atggcacagg ctattggcgc ccaggctgcg tttcccggca gacaagtcat
      421 ctcactctcc ggtgacggcg gcttcagcat gctcatggga gatttcatca gcctgacgca
      481 actcaaattg ccggtgaagg tcgtcgtctt caacaacggc acgcttggct tcgtcgagct
      541 cgagcaaaag tccacgggat tcctcgattt tggagtggac ttcgagaacc cgaatttcgc
      601 ggccatggcg aaagccatgg gcatctacgg cgtgcgcctg gaagaccccg ccgatgttga
      661 acgcggcatt gcggcggcgc tcgcccacga cggccccgtc ctgattgatg ccgtcgtgaa
      721 ccgcacggag ctggcgatgc cgccgtccat cacgctcgag atggcgaagg gcttcacact
      781 cttcatggtg aaggccgtga tgagcggccg ggccgaggag gtcatcgacc tcgccaagac
      841 gaacctatgg cgctgaaggc agacgtgaga tggagctcag agcactcttg taaggctctg
      901 catcgcagtg agctcgcggt gttcagtgcc gaggatgacc acgtcccatt gggattgaga
      961 ggttagccat gtctacacaa acaattccca gcggcggagc caagccgacg gacgcgagtg
     1021 ccttccaaac ggctttcatc atcgcctggc ttttctgcct gttgttctac ttcatggagt
     1081 acgccgtgcg ctcggcaccc agcgtcatgc tgcccgagct cagggccgcg ttcggcctga
     1141 acacagcggg cctaagctcg ttgatcgggc tttactatta ttcgtacgcg ggcttcgcca
     1201 ttgtcgcggg cgcgtcggtc gaccgttggg gcgcgaaata cacgataccg gccggcgtgt
     1261 tgatccttgc cgcgggcacc gcgatgttcg gcgtgaatgt gagctggatc gccggcgtgg
     1321 ggcggttcct gcagggcgcc ggtgccgcgt tcgcgtttgt cgcagcggtt tatcttgcgg
     1381 cccgggtctc ccggcgcgtt atctcgcgac cgcaatcgga attacccaat gcgtcggcat
     1441 gctcggcggg tccgcgggcc aattcgctgt cgctcccctc attcacggag cactcgaatg
     1501 gcaccaattc tgggtttatg cgggcgtcct gacgttattc atcgccgtcg cgatgctcat
     1561 tgctacgccg cgtgaacatt tatcccaagg cgcaaatgcg agcatctgga ccacgtttgc
     1621 gccctacaag atcgtcttga cgaacccgca gtcctatctc tgcgggctcg tcggggggtt
     1681 gcttttcctt cccacgacgg tcttcgccat gacctggggc gtatcgttcc tgcaggaggg
     1741 ctggcagctg gactacccac aagcggtcga tcgcgcagcg tcggtggcaa tgggctgggt
     1801 gttcggctgc ccgcttctcg gatacatcgc ggaccgcatc ggacgccgca agccggtgat
     1861 actcgcggga gcggcattga tgctggcggc gacgctggcg atcttttatc tgccgcccga
     1921 gacgtctcct ccatacgtgc tcggcttcct ccttggcgtc gggtccggcg cggcgatgat
     1981 cccctattca atcatcaagg aggtgaaccc ggatcatgcg aagggcagcg ccacgggtgc
     2041 gatgaacttc ctggtttttg tgatgacggc attggcagcg cctgcattcg gctggtggat
     2101 gcaaaagctc gcgggcggca cggcgttaac gctggaggtg ttcgacatcg ccggcagcgt
     2161 ctatgtcgcc gcgattgttg ccgccgcgat ccttacgctc ttccttaagg aaaccgggtc
     2221 cgctgttcgg gcgacgaatt gatgcgagcc tgcgagatgg ccttctcggc atgctcgtga
     2281 actgagtggg tcgacttggt caggagggca gggtggacgc atcagcataa gttgcatgac
     2341 cgaaacggac gagcagttgt ggccgctatt acaccatcga ctttatccat ctaacgcccg
     2401 aaggccacgc catgatcgcc gcgccttgct tccgcgtgtc gtggcaatcc tcgaatcggg
     2461 gagcccaatc ggcgccgaag cgattgaccc gggttttccg cgacttaagt attgcatcga
     2521 ggagagatcc catgggccaa acgcgaaaag agagcgacag cctcggcgtg gtcgaggtgc
     2581 ccgcggacaa gctgtggggg gcgcaaaccc agcgctcgct cgagcatttc agcatcggca
     2641 gggagttgat cccgcccgaa atgatcaccg cctacgccat cttgaagaaa gccgccgccg
     2701 ccgccaacca cgccggcaag cggctcgacg atcagcgcta taaattgatc gttcaggtgt
     2761 gcgacgaaat cctcgccggg aagcaccacg acatgtttcc cttgcacgtt tggatgacgg
     2821 ggagcggcac ccagttcaac atgaacgtca acgaagtgat ctccaaccgc tgctcccaac
     2881 tcgccggcac gccgctcggg agcaaaacgc ccgttcatcc caacgatcat gtcaacatgg
     2941 cgcagtcgtc gaacgacacg tttccgtcgg cgatgtacat cgcagccgcc gttaacgtga
     3001 agcgcagcct cctcccggcg gtcgccgcct tgcgcgacgc gatcagcgcc aaggcgcaag
     3061 cctgggatga catcgtcaag atcggtcgca cgcacatgca ggacgcgacc ccgctgaccc
     3121 tgggccagga atggtccggc tacgccggga tgctgtcgga tgatctcgaa cgcatcgaag
     3181 ccgcgctgca gggggtgtac cagttggcgc tgggcggcac cgcggtggga acgggcatca
     3241 actcggcacc gggctttgcc gaagcggccg cagccgagat cgccaagctc accggcctgc
     3301 cgttcgtcac cgcgcccaac aaattcactg tgcagggcgc ccacgacgcg ctggttcaac
     3361 tgtcgggaac gctccgcacg ctggcggtat cgctctacaa gatcgccaac gacattcgcc
     3421 tgatgtcgtg cggacctcgc gccggatttg cggaactgtc gattccggaa aacgagccgg
     3481 gctcctcgat catgccgggc aaggtcaatc cgacccagtg cgaagcgctc gcgatgctcg
     3541 ccgtgcaggt gatgggcaat gatgtcgcgg tgggcttcgg cggcgctagc ggctatctcg
     3601 agatgaacgt ctacaagccg ctgatgatct tcaacgccat ccactccatt atcctgatga
     3661 gcgacggctg cgtgaacttc cgcaagttcc tggtcgaggg caccaagccc aatctgaaga
     3721 agatcaacga atacgtcgag cgctcgctga tgctggtgac ggcgctgtcg cccgtcatcg
     3781 gctacgacaa ggcctcgaag gtcgcccacc acgccatgga caacgatctc acgctgaaag
     3841 aggccgcgtt gcaactgggc ttcgtcaccg aggcggaatt cgaccgcatc gtcgatccga
     3901 agaaaatggt caagccctac gtggcgagcg aagcatcgac caaggggccg cattgacatc
     3961 ttgcacgtcc ggcgctcgac gtggctcgcc gccttccgcc cggccggcca tgaggccggg
     4021 gccttcgaat ccgcaacttg cgagaagctg acatgacgtt ggcgcagcgt tgcaacctcg
     4081 tcctcgcctt cgcgcgcgtg ctctacgtca acggccaagc caccgagcag accgtggccg
     4141 cg
//
LOCUS       DATLJT010001034         5477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_301790,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001034 DATLJT010000000
VERSION     DATLJT010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301790"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VM910_23740"
     CDS             <1..312
                     /locus_tag="VM910_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI65565.1"
                     /translation="PKRKRHSRLEIATKLAQADELATQGKLQSEIARTLGVSVMTLHR
                     WHKAPRGPQRALVVTREAKEPDRTRAADDRIAKLQLENSRLRQLVTDLLLEKIKVVAS
                     G"
     gene            complement(545..1468)
                     /locus_tag="VM910_23745"
     CDS             complement(545..1468)
                     /locus_tag="VM910_23745"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65566.1"
                     /translation="MKGLLAVVLILPSMMSAAAVAQENAPGPRPVLPLAQRIAHTDPA
                     KYRHMLAAHDGPGALDYTALLDAETLDTNLFFLHRGIIEPKSGIGAHFHNQCEEMFII
                     LDAEAQFTIDGRTSLLEGPAGAPTRLGHSHAIYNASDRPVQWMNINIGSFKGVYDAFD
                     LADPRVGVPLDAIPTFITMQLDRALLKPVDHMNGGYGTVQYRRVLEPSVFYTTWSYVD
                     HLLLPPGSSSGPDTKPEMSEVYYVLDGDGTATVASESAQIHTGDAIPVRLNESKSFSN
                     TGSGPLEFMVFGIARDMAERQALMAAPRGNR"
     gene            complement(1643..1963)
                     /locus_tag="VM910_23750"
     CDS             complement(1643..1963)
                     /locus_tag="VM910_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65567.1"
                     /translation="MRRREFITLVGGAVAWPLADARAQQPAMPVIGFVSGRSPNESTG
                     VVAAFRRGLGETGFVEGQNLVIAFRWAEGRYHQLPALAGELVGLRVGVLVAAGGTASA
                     LAAK"
     gene            complement(2178..2318)
                     /locus_tag="VM910_23755"
     CDS             complement(2178..2318)
                     /locus_tag="VM910_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65568.1"
                     /translation="MKAAAAPVGAPWLWTILSYENRQPMHGYKVTREAAMGAFAKSWR
                     RE"
     gene            complement(2491..2760)
                     /locus_tag="VM910_23760"
     CDS             complement(2491..2760)
                     /locus_tag="VM910_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65569.1"
                     /translation="MRLDYCCNDDRAEDPEPGAWSREALEAMDARFVAALEQAFELGL
                     ESRTSAAGEIKLKPSLGPRRVTPLTREIQEGLWRSSASVVFVARS"
     gene            complement(3341..4561)
                     /locus_tag="VM910_23765"
     CDS             complement(3341..4561)
                     /locus_tag="VM910_23765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HVI65570.1"
                     /translation="MKVTKDSVIKLVLPPGKSEHVEWDDDIAGFGVRLRPHSATFIFR
                     YRRGKRQPCVTIGKVSAISMAQARSAAADLYARVRLGEDPAGDRSEARARANETFALA
                     LKPYLVRKQAELRPRSFANLDRHLSKHAAPLHRLELAKAAERRTVATLLAKIASSRGP
                     VEANSMKGSLSGFFVWVIGQGLLDGTGTDIDPTIGMANASTKGPRTHVPTDAEIAKIW
                     NALRNDDYSDIVRLLTLTLCRLSEIGNLTWDEVDFAAGLIRLPAARTKTGVARDVPMS
                     APVRRILEARQQHADSGRALVFGTGQGGFAGWTRAKAALDRRSGVVDWTHHDLRRYGS
                     TTMNNEGIALPHIIEAVLGHAVGDKIARTYNLAAYPEQVRAALELWGGRVMGLVAGER
                     KPAKVLALRRRKGA"
     gene            5232..>5477
                     /locus_tag="VM910_23770"
     CDS             5232..>5477
                     /locus_tag="VM910_23770"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI65571.1"
                     /translation="MTQLMVYQLAARTDAGVAGRHESYMAKIAGTELGFRVADRCLQI
                     HGGMGLATEMPISQMWRDARSFMITEGPVEVMRMVLAR"
BASE COUNT         1041 a         1651 c         1735 g         1050 t
ORIGIN      
        1 cctaaaagga aaaggcactc gaggctcgaa atcgcgacga agttggcgca agcagatgaa
       61 ttggcaacac aagggaaact gcaaagcgaa attgcgcgca cacttggcgt tagtgtcatg
      121 accttgcatc ggtggcacaa ggcaccgcgt gggcctcagc gcgcattggt ggtgacccgc
      181 gaggcgaaag agcccgatcg gacgcgcgcc gctgacgacc gaattgccaa actccaactc
      241 gaaaactcac ggttgcggca gctggtgaca gatctacttc ttgagaaaat caaggtcgtc
      301 gcgtccggat gatatggagt gttggcgaga cgcctcagcc agccgcaact ggcgcacata
      361 gcacggcagg catgggcgaa gacggtttaa gggcaatacg cccggtccgc gctccagcag
      421 gcgacccaac ggtcgcaagc tgctgcaaag ccataatagg aaatcactct aatcttaggg
      481 ccgccggggg gccgaaaaat gcgccaactt ttttcggtcg agcgcgaggg cggcggctac
      541 tgaactagcg gttgccgcgc ggggcagcca taagcgcctg cctctcagcc atatcgcgag
      601 cgatgccgaa aaccatgaat tcgagcgggc cgctgccagt attggaaaat gatttgcttt
      661 catttagccg gactgggatc gcatcgccgg tgtggatttg agcgctctcc gaagcgacgg
      721 tcgcagtgcc gtccccgtca agaacatagt agacctcgct catctctggt ttcgtatcgg
      781 gaccactcga actacctggc gggagaagta gatgatcgac atacgaccaa gttgtgtaaa
      841 agacgctggg ctcgagcaca cgacgatatt gcacggtccc gtagccgccg ttcatatgat
      901 ctaccggctt gagcagcgca cggtcaagtt gcatggtgat gaatgtagga atcgcgtcca
      961 gtggaacacc tacccgagga tcagcgagat cgaaggcatc ataaaccccc ttgaacgaac
     1021 cgatattgat attcatccac tgtactggcc ggtcgctcgc gttgtagatg gcatgagaat
     1081 ggcctaggcg tgtaggcgcg cccgcgggac cttcgagcaa tgatgtgcgg ccgtcgatcg
     1141 taaactgagc ttcggcgtcg aggatgatga acatttcctc gcactggttg tgaaaatgcg
     1201 cgccaatacc gctctttggc tcaatgattc cgcgatgaag gaaaaacaga ttcgtatcga
     1261 gcgtttccgc atctaagagt gctgtgtagt ccagcgcacc cgggccatcg tgtgctgcga
     1321 gcatgtggcg atatttcgcc ggatcggtat gggcgatgcg ttgcgccagg ggcaaaaccg
     1381 ggcgcggtcc cggggcattt tcttgagcaa ctgccgcagc ggacatcatc gatggaagta
     1441 tcaggacaac tgcgagaaga cctttcattt tagtttaatc ctattccgga ttgagcctat
     1501 cttcaccgag agcctcaaga agctggttga tggggcgtgt caaatgtcgg acgcggtaac
     1561 gaatgtaggg tcagcctcga agaggccgca gcatcgacga gcgataacca cgcctgatgt
     1621 gcgatggggc aatagcgcaa tactacttgg cagcaagcgc cgatgcggtg ccccctgccg
     1681 cgacgaggac gcccacccgc aggccgacga gttcccctgc gagcgcgggc aattggtgat
     1741 aacgaccttc cgcccagcga aacgctatca ccagattctg tccttcgaca aacccggttt
     1801 cccccagccc tcggcggaag gctgcaacta ctcccgtcga ctcgtttggt gatcggccgc
     1861 tgacgaaccc gatcaccggc atcgccggct gctgcgcgcg cgcgtccgcc agcggccacg
     1921 cgaccgcgcc gccgaccagc gtgatgaact cccggcgtct catagtcggc cccaggggtc
     1981 aaaaccacgc accgcatcgt ctcacagccg tccgagtctt ggaacggggt gagaggggtg
     2041 cgaactgcga ccaattgttc tgggctggga atgtcggctt tgagtcccac gaccggggtc
     2101 aaaaccggaa aagctcaaaa tcagcaaacg tttttcggtt tgccccccga aagcggatct
     2161 acctcccgat tttagaacta ctcccgccgc cagcttttgg cgaacgcccc catcgcggcc
     2221 tcgcgcgtca ccttgtagcc gtgcatcggc tgacgatttt cgtaggacag gatggtccac
     2281 agccatggcg ctccaaccgg ggccgctgcc gctttcatga tgcggccgac cgccgtggcc
     2341 gttatactga ggaagagtat cagcggcttt ccgcggaatt tgtaagagct gcgcgtgaag
     2401 ggcgtgtcgt tggtgctgag ccgctgaacg gggtaggggc gctgtggtga aggggggcgc
     2461 acggttggat gacggaattc cggtgtcgcg ctaactccga gcgacgaaca ccacgcttgc
     2521 gctcgagcgc cacagccctt cttggatttc ccgcgtcagc ggcgtcaccc ggcgcgggcc
     2581 gaggcttggc ttaagtttaa tctcaccggc tgcgctcgtt cggctctcca atcccagctc
     2641 aaaagcctgc tctaacgccg cgacgaaccg tgcatccatt gcctcgagcg cttccctgct
     2701 ccaggcgccg ggttcgggat cctcggcgcg atcgtcgttg cagcaatagt cgagtcgcat
     2761 gaagggcagc gtggggaagc tcgaccgaca aatcaatcac ccggtgctcg gcttcgacgg
     2821 gcgacctcgc gccctgcggg gcagcagaag atcggccatg atcagactgg ctcgcgagcc
     2881 gggaggagcg gtcagccgct cgcgcgcagc ttctctcgct cttgggtcca ccgtcgcatc
     2941 tgtcgggtag acttgcgatc gcgaagacgt ttcgccagcg cccaccgaag ttgattgatc
     3001 gcccgatcgg tcttgagaac gtcgcaaagc agcccggcaa gcaggaagaa attttcgtcg
     3061 atcgtgaccc agttccaaac cgagttgagt cttgaaagta tcccttccga agagccgctg
     3121 tcgcattagc agggtggcga tgcgcgccac ggtacgccta cgagcggcat ggcgcttgcg
     3181 ccaggcacgc tgcttctcgg cgttggtcag cgccatgatc gacccttctc ggttacggcg
     3241 taaccgtacc tcaaggggat caccccgtcg aacgcagcca gcgccactaa gatttgctcc
     3301 cgattttcga gcctcacggc ggcgggctgc caaacggcgg ttaggcgccc ttccgccgcc
     3361 gcagcgcaag gaccttcgcg ggcttccgct cgcccgcaac cagtcccatg acgcggccgc
     3421 cccagagctc gagcgcggcg cggacctgtt ctggataggc ggccaagtta taggtacggg
     3481 cgatcttgtc gccgacggca tggccgagca cggcctcgat gatgtgcggc agggcgatgc
     3541 cttcgttatt catcgtggtc gagccgtaac gacgtagatc atggtgcgtc cagtcgacga
     3601 cgccggagcg ccgatcgagc gcagccttgg ctcgtgtcca gccggcaaac ccgccctggc
     3661 cggtgccgaa caccaacgcc cggccactat ccgcgtgctg ctggcgcgct tccaggatac
     3721 gccgcaccgg cgccgacatc gggacatcgc gggcgacgcc ggtcttggtc cgcgccgcgg
     3781 gaagccggat cagcccggcc gcgaagtcga cttcgtccca ggtcaagttg ccgatctcgg
     3841 acagccggca aagcgtgagc gttagcagcc ggacgatgtc gctgtagtcg tcattacgaa
     3901 gggcgttcca gatcttcgcg atctcggcgt cggtcggcac atgcgtccgc ggtcccttgg
     3961 tcgacgcgtt ggccatgccg atcgtggggt cgatatcggt gccggtgccg tcgagcagcc
     4021 cttgcccgat cacccagacg aagaagccgg agagtgagcc cttcatgctg ttcgcttcca
     4081 ccgggccgcg ggaagaggcg atcttggcga gcagcgtcgc cacggtgcgt cgttcggccg
     4141 ccttggcgag ctctaggcgg tgcaacgggg cagcgtgctt cgaaagatgg cgatcgaggt
     4201 tggcgaagct tcgcggccgc agctcggcct gcttgcgaac gaggtagggc ttgagcgcca
     4261 gcgcgaaggt ctcatttgcc cgcgcgcgcg cctcggagcg atcgccggcg gggtcctcac
     4321 ccagccggac gcgggcatag aggtccgcgg cggcggagcg cgcctgcgcc atcgagatcg
     4381 cggacacctt gccgatggtg acgcacggtt ggcgcttgcc gcggcggtag cggaagataa
     4441 acgtcgcgct atggggccgc aggcgtacgc cgaagccggc aatatcgtcg tcccattcga
     4501 cgtgttcgga cttgccgggc ggcagcacga gcttgatgac gctgtctttg gtgaccttca
     4561 tgttcgagcc gttggttaca gcggtcgctt agatgccgcg gctgcggcga tgtctttgat
     4621 gtcctgcgtc gagctcgtaa ccttgactgg gatctgtggc agccggctgc tggtcttgcg
     4681 ccaagagatc atgaagcgaa ctggcaacgg tggtgcgtct tccgggactt tggtgggatc
     4741 gcggagagaa atgagtggca ggtcgcaggt ttcgcctggc cgcagcagga ccttggcatc
     4801 gatgttgacc tgggccctcg ttattgctcc aggtgagtga tccctggcaa tgcagtagat
     4861 ccccggtgga cgcgcttgca caccccgaat gagcacactt ccggggccgg gattttggat
     4921 gtgaatgtac tcgtagggtc ttgtccccac tattggcttt gcgatcactt ccgcctgcgg
     4981 tcgccctcgc gccaagcgac caatcgcagg tccgaggacg gagccagcta ttttgatgat
     5041 gacttcaatc atgctgtgtc gagtgccagt tgcaccacgc tattgcgtcc ttcaggcgcc
     5101 tcgtggagag cacatggaga gcagccgtat tcggttgcct gccccctcgt acccccctct
     5161 tagctgatgg tacaagagaa attacaggct tgtgcgccct tgtgcgttct tgtgcggtcg
     5221 cgtggtatca catgacgcag ctgatggtgt accagctcgc cgcgcgcacc gacgccggcg
     5281 ttgccggccg gcacgaaagc tatatggcca agatcgcggg caccgagctc ggcttccgcg
     5341 tcgccgaccg ctgcctgcag attcacggcg gcatggggct cgcgaccgaa atgccgatct
     5401 cgcagatgtg gcgcgacgcg cgcagcttca tgatcaccga gggtccggtg gaggtgatgc
     5461 gaatggtgct tgcgcgc
//
LOCUS       DATLJT010001035        17536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_531386,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001035 DATLJT010000000
VERSION     DATLJT010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531386"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VM910_23775"
     CDS             <1..638
                     /locus_tag="VM910_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI65572.1"
                     /translation="AKKHDGISFILFDMASKGVSTKPILLISGRSPFCETFFDNVRVP
                     KANVVGQVNRGWDVAKYLLQHERAMISGVGERSVARPLGQIAADSVGSDEQGRLEDPV
                     LRGQIASFEVDEAALAAAAERMVDLAKAGQGHPAFSSAMKYYGTELNKRRYEILMSAG
                     GIDALEWDSARSKGGARARAWLRTKANSIEGGTSEVMLGIVAKRILDLPGA"
     gene            713..1852
                     /locus_tag="VM910_23780"
     CDS             713..1852
                     /locus_tag="VM910_23780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI65573.1"
                     /translation="MALVLSEEQSMLRDSARGLISDKAPVSHLRHLRDSKDATGFSRE
                     LWKSFAEMGFAGLLVPEQFGGSGLGCVEAGIVMEEIGRTLMPSPFLSTSVLAASALSR
                     GGSAAQKSEHLPKISAGQLLAALAVDEGTKHRPLQTKLQAVRSGNGFKLNGSKAFVVD
                     GHVADLLIVAARTAGSAGERNGLTLFLVDPRSKGIATERTIMVDSHNAARIDFENVEV
                     NADHVLGEVDQGFALLDGVLNIGRVAVASELVGLSEEVFGRTVSYLKERKQFGKAIGE
                     FQALQHRAAQLYIDIEITRAAVLKAHQTLDVDFANAASAGAVAKARAGTTAARAVQEG
                     VQMHGGMGMTDQFDIGFFMKRARVCEELFGDANFHADQLARSRSY"
     gene            1965..2936
                     /locus_tag="VM910_23785"
     CDS             1965..2936
                     /locus_tag="VM910_23785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65574.1"
                     /translation="MSCVHRRQFVALLGLAAVRPLAARAQQQFRIGLLNTGASSFFIP
                     PFKQKLEELGYAEGKNLIIERKFAEGSSERLNEFAQDFVREHVDVIVTIGTPAGFAAT
                     RATSTIPIVLGAISDPVGLGLVKSLARPGGNATGNSLMAPELSAKRLEILRMLAPGIS
                     RFAILWDSSNPGMAERVHETQVAADQTHVLLHVVGPRNVEELEAAFADLLRQRPDALL
                     VTTEAFTRQHLSRILDFANQNKIPAMFEDSFFVEAGGLMSYGPNYEEVFRTAAVFVDK
                     ILKGARPADLPVEQPTKFQLVLNLKTAKALAIDIPPNLLTMADRVIE"
     gene            3010..4260
                     /locus_tag="VM910_23790"
     CDS             3010..4260
                     /locus_tag="VM910_23790"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVI65575.1"
                     /translation="MMNRRTFCALAGAMVAAPRIVWGQGMTSKAFFYVSLGPALTLYR
                     IDVDAAALSKGSTVTLPEKVQYAWPHPSAPFLYVASSNGGASSLGVKGDKHFLSALRI
                     DANNGELLAHGPPAALRSRPIHVTVDHAGAFALVAYNDPSSVTVHRINADGSVGEEVK
                     QAASLDCGIFAHQIRVTPRNDAAILVCRGNDPAKGRPEDPGSLKLFSFKDGQLANLMS
                     VAPNGGYGFGPRHLDFHPSLPLVYVSRERENKLDVYRLSNGVLDAQPIFVKDTLAEPG
                     KLRSRQAACTLHFHPNGRSLYLANRAFDTVKIDGKDIFPGGENNIAVFAIDPATGEPT
                     LIQNEDVRGVYPRTFALDAGARMLVATNVEPKLVRDGTGLKTVPANIATFRVGTDGKL
                     AFANSYEAETGGDTQFWSGMVTLA"
     gene            complement(4335..5372)
                     /locus_tag="VM910_23795"
     CDS             complement(4335..5372)
                     /locus_tag="VM910_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI65576.1"
                     /translation="MRTFRGGLLIGLAVAVLVGAVAVAYQLYDTRTLKRTIRRGEVLC
                     GVNKGLPGFSIPDDKGNWTGFDVDFCRAVAAAIFDDPTKAKFVPLDASERFKELQNRR
                     VDILSRNSTWSMSREVNYDLYFPAVAYYDGQGFMLPRARNIDSALDLNGSKVCVQAST
                     TTELNLADYFKANNMKYEEMKFPQLDEVVKAYESGKCDTFTADISQLHALRLNLAKPN
                     DHVILPDVISKEPLAPVVRQRDDDWMMIVKWTLYAMINAEELGVTSKNIDEALKSKKP
                     DVMRLVGNEGSYGEDLGLSKDWAVRIIRHVGNYGEVYERNVGSESNLHIPRGLNQLWN
                     AGGIQYAPPIR"
     gene            complement(5426..6019)
                     /locus_tag="VM910_23800"
     CDS             complement(5426..6019)
                     /locus_tag="VM910_23800"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI65577.1"
                     /translation="MTKLRRLIWMSMAAVGLMLSASQTFAQGQDPGDQPGLMADESFQ
                     LDPEWQKQVVLYRSTEAPGTIIISTAERHLYLIQPGGRAIRYGIGVGRDGFQWQGLLN
                     ISKKAEWPDWTPPPEMIARQPYLPRFMAGGPGNPLGARAMYLGATVYRIHGTNQPWTI
                     GTKISSGCFRLVNTDVADLYDRVPVGTKVVVRQKPEL"
     gene            complement(6166..6294)
                     /locus_tag="VM910_23805"
     CDS             complement(6166..6294)
                     /locus_tag="VM910_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HVI65578.1"
                     /translation="MPGEPTLRPLWLRLLWFAGLWLAGVGTVALLSLLLHLWLAPK"
     gene            complement(6294..7304)
                     /gene="cydB"
                     /locus_tag="VM910_23810"
     CDS             complement(6294..7304)
                     /gene="cydB"
                     /locus_tag="VM910_23810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVI65579.1"
                     /translation="MTAAIDIATVWAFIIAFAVFVYVVMDGFDLGLGILFPLFPAKGD
                     RDTIMNTVAPVWDGNETWLVLGGGGMMAAFPLAYAVLMPALYTPVIAMLIGLIFRGVA
                     FEFRWRVKSERNRWDLAFAGGSWLATLAQGVALGAILQGVHVEERHYAGGWWDWLTPF
                     SILTGVSLAIGYALLGATWLVLKTEGPLRDRAYHLAWYLLFAMLLAIGAVSIATPFLH
                     IQYAERWFTWPNIVLTAPVPIAVAVVTVALLRSLADKQDTRPFFLTLLLFALSYLGLG
                     ISMYPYIVPQSIDIWQAASPANSQIFMLFGVAVLIPLILAYTGWAYWVFRGKVRAGSG
                     YH"
     gene            complement(7301..8719)
                     /locus_tag="VM910_23815"
     CDS             complement(7301..8719)
                     /locus_tag="VM910_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVI65580.1"
                     /translation="MSSAMSWLISGFEAVSLARAQFAFTMSFHIVFPAFSIGLASYLA
                     VLEALWLWTGREVFLNLFNYWLKIFAVAFGMGVVSGIVMSYQFGTNWSAFADKTGPVI
                     GPLMAYEVLTAFFLEAGFLGVMLFGLERVGRRLHFVATLMVAIGTLVSAFWILSANSW
                     MQTPAGYAVNPAGQFVAADWLKVIFNPSFPYRLVHMVLAAYLTTALVVGAAGAFHLWR
                     DRHLAGARVMFSMAMWMATLVAPIQLIAGDQHGLNTLKYQPVKIMGMEGHFESHPDGA
                     PLYLFGLPDEKAGKVKYAIEIPRMGSLILEHDLNAPMKGLNTVPRENWPPVPITFWSF
                     RIMVGMGFLMFGLGLLSLWARWRGTLYEWRLLHLFAIVMGPAGFVAVLAGWITTETGR
                     QPFTVYGLLRTADSASPLAAPAVGASLISFAIVYFVVFIAGLIYLLRLMALPPHPGEA
                     GLPRDIPARAAGITPAIESVAR"
     gene            complement(9061..9672)
                     /locus_tag="VM910_23820"
     CDS             complement(9061..9672)
                     /locus_tag="VM910_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI65581.1"
                     /translation="MQNVVAINAAARQSIIAAQATSDEMLLESIADGSKTAMHILYAR
                     HNVRVYRFILRIVRDTTMAEDLVSQVFLDVWRTASQFEGRSQVSTWLLSIARFKALTS
                     LRQRRFEDIDQDDVREIADEADTPEASLDRSTTSEILRACVAKLSPAHREIINLVYYH
                     EKSVEEVGEIIGIPQSTVKTRMFYARKQLADLLKGAGVDSIAA"
     gene            complement(9973..10719)
                     /locus_tag="VM910_23825"
     CDS             complement(9973..10719)
                     /locus_tag="VM910_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI65582.1"
                     /translation="MSQSQHIIIVDDEAPAREMVGDYLKMHGFTVTLCDGGKSLRSAI
                     ETGAPPDLVVLDLNMPEEDGLSIIRDLKSRTNVPVIMLTATASPIDRVVGLELGADDY
                     VAKPCELRELMARIRSVLRRSAPAKAAPAASEAASAKTAKDQLVRFGTKWLDLEAQAL
                     RDDEGNEHPLTASEFGLLKVFAANPKRVLSRERLLELANARDSEAFDRAVDLRIMRIR
                     RKIEPDPTKPAVIRTIRGGGYLFSPTGEKA"
     gene            complement(10722..12464)
                     /locus_tag="VM910_23830"
     CDS             complement(10722..12464)
                     /locus_tag="VM910_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HVI65583.1"
                     /translation="MAEQDDVLHLIDDTDAAREEAPARKWKIAVIDDDHAVHEGTRFA
                     LSDYSLNGQGLEILSAYSAAEGRALMRAHPDIAAVLLDVIMETDVAGLELVEYIRNEI
                     RNETVRIILRTGQPGQAPERRVIVQYDINDYKAKTELTADKLFTSLTAALRSYQQLER
                     MVQTRRGLEIIIDAASTLYDFRSMQRLAEGVLTQLASLLNVDCAGILVLRDDGRKAND
                     EFSVLAGSGCYSRFIGSAGSKSLDPDLRTMVEAAFKRRKHEFHDHRTVLYVRTGSGRE
                     VVVLLQAERQLSETDRSLVEIFGSRLSIAFDNVILYQQLHEANTQLEDRVAQRTRALM
                     QANRRLSAQWLRLQRANGFKNEILGTVAHDLKNPLGVILGRTEMLTELITTSASKESI
                     TAQIDHIRDATRRLTTMVDHLISDAMADAFDITIRREPVDIAALVGEVAEANQPLAVN
                     KQQTISVSAPSNFVTVCDTDRIREAIDNLFSNAIKYSPIGGKISVTVAREDDNTVIRV
                     ADQGQGLSPEDLGRLFGRFQRLSAKPTAGESSTGLGLSIVKRIIDMHGGEVTARSDGP
                     GLGSTFTILLPATE"
     gene            complement(12434..14416)
                     /locus_tag="VM910_23835"
     CDS             complement(12434..14416)
                     /locus_tag="VM910_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI65584.1"
                     /translation="MRWRILSIAALNSAVVIVLAALIWNGAKVLGSAWEDVRQVRESD
                     KILARLESETSRLQNLIHRYINQPSPELFAEILLLREAVLGTLTTRASTDPMLAGSVE
                     ELERVTDRFLNGFGELRSLQATIAKTYEEQVLKPARDMAGLYSIIEGATGHRDALIWP
                     SLGKSREAFTAMLVAANSYYLSLATLSAEEARRNTETIEKTIPVMSDLADNDLQRLAL
                     QRLNTRTAALREGLAKLTEQLSTRTELLRNTIDATQAEEIGAIDELSTKMREREQKAQ
                     ETFDRTLSEISRRVLSIAVIFLGIILTAGVLIALSIRLPLQQIMTAMRAITLGDYDRE
                     VLGTTARDEVGAMARAVEVFRENAIAKRRTEDELRSSKEKAESALFELNTAQQNLIDA
                     ERLAALGGLVAGVAHEVNNPIGISLTVASSLARRTEMFEAELRSEGGLRRSQLEEFVR
                     TSRDASQQLVANLHRAGELIQSFKQVAVDRSHAERRQFSLGEATDQIVASLRPVLKRA
                     PITLNVDVPEGLIINGYPGSYGQILTNLFLNAVNHAFADGRSGTITISARARGHDDVE
                     INFADNGVGMTPDVQRQAFDPFFTTRRNEGGTGLGLHIVYNLVTQQLGGRMMLESRPG
                     QGTTFRIIMPRTAKGGTSDMVADGSAEWPNRTMSST"
     gene            complement(14521..15513)
                     /locus_tag="VM910_23840"
     CDS             complement(14521..15513)
                     /locus_tag="VM910_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI65585.1"
                     /translation="MPVYRADLPRALAVIVALLCAVATTGVFAREFRAADTQSEDYPT
                     VQALRYMGRLIEERSGGRLQIRVFHSRQLGEEKETIEQTRVGAIDLNRTNVALVGTFV
                     PATNVLAMPFLFRSIEHLQKVLDGPIGNEILNSFEPYGFVGLAFYDSGARSIYNSVRP
                     IRSLADLKGLRIRVQQSELMAEMIRAFGAEPVQMPYGQVLTGLATRLIDGAENNWPSF
                     VTTDHYKYAGYYTITEHTMTPEVLVMSLKAWQSLSDDDRNIFREAALRSSQFMREKWR
                     DLEERSRQQAEAAGVEIVKDLDREPFIAAMSVVYEKARRDPATANLIDRIRNAE"
     gene            15738..17075
                     /gene="ugpB"
                     /locus_tag="VM910_23845"
     CDS             15738..17075
                     /gene="ugpB"
                     /locus_tag="VM910_23845"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HVI65586.1"
                     /translation="MVYTVKLALRVLQLLVVLLAIGVVSPAFAVTDIMWWHAMSGQLG
                     RQVDKLAADFNASQSEYRIVATYKGNYTETVTAAIFAFRSRSQPAIVQVNEIATATMM
                     AAKGAIYPVYDLMRDAQEPFSPTAYLPAVTGYYADAGGNLLSFPFNASTPILYYNKTM
                     FRAAGLDPESPPKTWPELGQAAKRLREHGAACGFTTSWPSWINVENFSAFHNLPIATR
                     ANGLDGLDAKLTFNNPTVVRHVAQLAEWQTTKVFDYSGRAQSAEPRFQNGECGIFIGS
                     SATRADIKANSKFEVGYGMMPYWPDVAGAPQNTIIGGATLWVLRDRPRAEYRGVAKFF
                     AFLSKPEVQAAWHQNTGYLPITRAAFELSRSQGFYDRNPGAAISIEQITLKPPTENSR
                     GLRLGSFVLIRDAIEEELEQAFAGKKSAQAAMDAAVERGNKLLRQFERASPDR"
     gene            17319..>17536
                     /locus_tag="VM910_23850"
     CDS             17319..>17536
                     /locus_tag="VM910_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI65587.1"
                     /translation="MKRRDFLKSVSGLAAGALVPAPAIISPAKADARSETLLIVSEGG
                     PNNLDIHGVGTNVPGYEVSWNCYDRLIS"
BASE COUNT         3281 a         5310 c         5663 g         3282 t
ORIGIN      
        1 cggccaagaa gcatgacggc atcagcttca tcttgttcga catggcttca aagggcgtct
       61 ccaccaagcc gatccttctc atctccggcc gctcgccgtt ttgcgaaaca ttcttcgaca
      121 atgtccgcgt gcccaaggcc aacgtcgtcg gtcaggtcaa ccgcggctgg gatgtcgcga
      181 aatacctgtt gcagcacgag cgcgcgatga tctcgggcgt cggcgagcgc agtgtcgccc
      241 ggccactcgg ccagatcgcc gcggattccg tcggctccga cgagcagggc cggctggaag
      301 atccggtgct gcgcggccag atcgcaagct tcgaggtcga cgaggccgcg cttgccgcgg
      361 ccgccgagcg gatggtcgat ctcgccaagg cggggcaggg gcatccggcg ttttcctcgg
      421 cgatgaaata ttacggcacc gagctcaaca aacgccgcta cgaaatcctg atgtcggccg
      481 gcggcatcga tgcgctggaa tgggacagcg cgcgctccaa aggcggggcc cgcgcgcgcg
      541 cctggttgcg caccaaggcc aattcgatcg agggcggcac gtcggaggtg atgctcggca
      601 tcgtcgccaa gcgtatcctc gatctgccgg gggcgtagct tcttcacctg cccctgaaaa
      661 ggggaggccg tccgaattga aagagagttt ttccaatacc ggaaacccgc ccatggccct
      721 cgttctatca gaagaacaat cgatgctgcg cgacagtgcg cgcggtctca tctccgacaa
      781 ggcgccggtg tcgcatctgc gccacttgcg cgacagcaag gacgccaccg gtttttcccg
      841 ggagctctgg aagagctttg ccgagatggg attcgccggt ctcctggttc cggagcaatt
      901 cggaggcagc gggctcggct gcgtcgaagc cgggatcgtg atggaagaaa tcggccgcac
      961 cctgatgccg tcgccgtttc tctcgacctc ggtgttggct gcgtccgcgc tgtcgcgcgg
     1021 cggcagcgcc gcacagaaat ccgagcatct gccgaagatc tccgccggcc aattgctcgc
     1081 ggcgctcgcg gtcgatgagg ggacaaagca tcgtccgctg cagacgaagc tgcaggcggt
     1141 acgctccggc aacggattca agctcaacgg ctcgaaggcc ttcgtggtcg atggtcacgt
     1201 cgccgacctc ctcatcgtgg cggcgcggac ggcaggttct gccggggagc gcaacggact
     1261 gacgctgttt ttggtcgatc ccaggagcaa gggcatcgcc acagaacgca ccatcatggt
     1321 cgattcgcac aacgccgcgc gaatcgattt cgagaacgtc gaggtcaatg ccgatcatgt
     1381 gctcggcgag gtcgatcagg gctttgcgct gctcgatggc gtgctcaata tcgggcgtgt
     1441 cgcagtcgcg agcgaactgg tgggcttgag cgaagaggtg ttcggccgca ccgtgtccta
     1501 cctcaaggag cgcaagcagt tcggcaaggc catcggcgaa ttccaggcgc tgcagcatcg
     1561 cgcggcgcaa ctctacatcg atatcgagat cacccgcgcc gcggtgttga aggcacacca
     1621 gacgctcgat gtggatttcg cgaatgcggc gagcgcgggt gcggtcgcga aggcgcgagc
     1681 cggcaccacc gccgcccgtg cggtgcagga aggcgtgcag atgcacggcg gtatgggcat
     1741 gaccgaccag ttcgacatcg gctttttcat gaagcgcgcc cgcgtgtgcg aagagctgtt
     1801 cggcgatgcc aatttccatg ccgaccagct tgcgcgttcg cggagttatt gacaggtcac
     1861 tcctgacgca tatcggcgcg aatgtttgat gacagtgcac ggcttttcgc cgcgccgtgt
     1921 tcgcaatgtg ctagtctgcg cggcgtgccc tgaggaccga tttcatgagt tgcgtgcacc
     1981 ggcggcagtt cgtcgcgctt cttggtctgg cggctgtccg gccgctcgcg gcgcgggcgc
     2041 agcagcaatt tagaataggg ctgctcaata cgggggcttc cagcttcttc atcccaccct
     2101 tcaagcagaa gctggaagaa ctcggctatg ccgagggcaa gaacctcatc atcgagcgca
     2161 agtttgcaga gggcagttcc gagcgtctga acgagtttgc gcaggatttc gttcgcgagc
     2221 atgtcgacgt cattgtgacg atcgggacgc cggccggttt tgcagcaacg cgagctacca
     2281 gtaccattcc catcgtttta ggagcaatca gtgaccccgt cgggctcggc ctggtaaaaa
     2341 gtctcgcccg gcccggcgga aacgctaccg gcaattcgct gatggcgccc gaactgagcg
     2401 caaagcggct cgagatactt cgcatgctgg ccccgggaat ttcccgcttt gcgatcctgt
     2461 gggactcgtc aaatccgggc atggccgaac gggtgcatga aacccaggtc gcggcggacc
     2521 agacgcacgt tctgttgcac gtcgtcggtc cgaggaatgt cgaagaactg gaagcggcgt
     2581 tcgccgacct gctccgacag cggcctgacg ccttgcttgt gacgaccgag gccttcacga
     2641 ggcagcatct ctctcgcatt ctcgactttg cgaatcagaa caagattccg gccatgtttg
     2701 aggacagctt tttcgtcgag gccggcgggt tgatgtctta cgggcccaac tacgaagaag
     2761 tgtttcggac ggcagccgtc tttgtggata agatcttgaa gggtgcgagg cctgccgatt
     2821 tgccggtcga gcagccgacc aagttccagc tcgttctgaa cctcaagacc gcgaaggcgc
     2881 tcgcgattga tattccgccg aacctgctga ccatggctga tcgagtcatt gagtgaacaa
     2941 tggtcggttc ttggcggccg atcgtgtagt ctggtgcggc tcaattgcga cgggatgaag
     3001 ggttcgacca tgatgaatcg tcggacgttc tgtgcgctcg cgggcgccat ggtcgcagcg
     3061 ccgcgtatcg tttggggtca gggaatgacc agcaaagctt tcttctatgt cagccttgga
     3121 ccggctttga cgctctaccg gatcgacgtc gatgcagcgg ctttgtcgaa aggcagcaca
     3181 gtgacgctgc cggaaaaggt ccagtatgcc tggccgcatc cgtccgctcc cttcctttat
     3241 gttgcgtcaa gcaacggcgg ggcgtcttcg ctcggcgtca agggcgacaa gcattttctg
     3301 agcgcgcttc gcatcgatgc gaataacgga gagctgttgg cgcacggtcc gccagccgcg
     3361 ctgcggtcgc gtccgatcca cgtgacggtc gatcatgccg gggcgttcgc gctggtcgcc
     3421 tacaacgatc cttcaagtgt cacggtgcat cggatcaatg cagacggcag cgtcggtgag
     3481 gaggtcaagc aggcggcttc tctcgattgc ggcatcttcg cccatcagat tcgcgtgacc
     3541 ccccgcaacg acgcggcgat cctggtgtgc cgcggcaacg accccgccaa aggaaggccg
     3601 gaggatcccg gctcgctcaa gctgttcagc ttcaaggacg gtcagctcgc caacctgatg
     3661 tcagtcgcgc cgaacggcgg ctacggcttc gggccgcgcc atcttgattt tcacccgagc
     3721 ctgccgctcg tgtatgtgtc gcgcgaacgc gagaacaagc tcgatgtcta ccggctgtcg
     3781 aacggcgtgc tcgatgcgca gccgatcttt gtgaaggaca cgctggcaga gcccggcaag
     3841 ctccgctcac gccaggccgc gtgcacgctg catttccatc cgaacggtcg ttcgctctat
     3901 ttggccaatc gcgccttcga caccgtaaag atcgacggca aggacatctt tcccggtgga
     3961 gagaacaaca tcgccgtgtt tgcgatcgac ccggcgaccg gcgagccgac gctgatccag
     4021 aacgaggatg tgcgcggcgt ctatcccaga accttcgcgc tcgatgcagg cgccagaatg
     4081 ctggtagcca cgaacgtcga gcccaagctg gtccgcgacg gcacaggcct gaagaccgtt
     4141 cccgcgaaca tcgcgacctt ccgcgtcggg accgacggca aactcgcttt cgccaacagc
     4201 tacgaggccg aaacaggcgg cgatacgcag ttctggagcg ggatggtgac gcttgcgtag
     4261 ccgccggctg cgccgtgtcc cgcctgagtc gcccgacaag ccttcccacc ctcccctcca
     4321 gggagggtag accgtcaccg gatcggcgga gcgtactgga tgccgccggc gttccagagc
     4381 tggttcagcc cgcgcgggat gtgcaggttg gattcgctgc cgacgttgcg ctcgtacacc
     4441 tcgccgtaat tgccgacatg gcggatgatg cgcacggccc agtccttgga gaggccgaga
     4501 tcctcgccgt agctgccctc gttgccgacg aggcgcatta cgtccggctt cttcgacttc
     4561 agcgcctcgt cgatgttctt cgaggtgacg ccgagttctt cggcgttgat catcgcatag
     4621 agggtccact tcacgatcat catccagtcg tcgtcgcgct ggcgcaccac aggcgcgagc
     4681 ggctcctttg agatgacgtc gggcaggatc acgtgatcgt tgggcttggc gagattgagg
     4741 cgcagcgcat ggagctggga gatgtcggcg gtgaaggtgt cgcacttgcc ggactcatag
     4801 gctttgacca cctcgtccag ttgcgggaac ttcatctcct cgtatttcat gttgttggct
     4861 ttgaaatagt cggcaaggtt gagctcggtc gtcgtgctcg cctgcacgca gaccttgctg
     4921 ccgttgaggt cgagggccga atcgatgttg cgtgcgcggg gcagcatgaa accctggccg
     4981 tcgtaatagg cgaccgcggg gaaatagaga tcgtagttca cctcgcgcga catgctccag
     5041 gtcgagttgc gggagaggat gtccaccctg cggttctgca actccttgaa gcgctcactg
     5101 gcgtcgagcg ggacaaactt ggccttggtc ggatcgtcga aaatcgcggc cgccaccgcg
     5161 cggcagaagt cgacgtcaaa gccggtccag ttgcccttgt cgtcggggat cgagaaaccg
     5221 ggcaagccct tgttgacgcc gcacagcact tcgccgcgcc ggatcgtccg cttcaatgtg
     5281 cgcgtgtcgt aaagttggta ggcaacggcg acggcaccga ccaggactgc aaccgcgagc
     5341 ccgatcaaga ggccgcctcg aaacgtgcgc atcattttgg ttctcgcaat gatcgaagaa
     5401 aggcgcggga taaaggcggc cggagctaca gctccggctt ttgtcgcacg accaccttgg
     5461 tgcccacggg aacgcgatcg tagagatcgg cgacgtcggt attgacgagg cggaagcagc
     5521 ccgaagagat cttggtgccg atggtccagg gttggttggt gccgtggatg cggtacacgg
     5581 tggcgccgag atacatggcg cgggcgccga gcgggttgcc gggtccgccc gccatgaagc
     5641 gcggcagata gggctggcga gcgatcatct cgggcggcgg ggtccagtcc ggccactccg
     5701 ccttcttcga gatgttcaga agtccctgcc actggaatcc gtcgcggccg acgccgatgc
     5761 cgtaacggat ggcgcgcccg ccgggctgga tcagatagag gtggcgctcg gcggtcgaga
     5821 tgatgatggt gccgggcgcc tccgtggagc ggtaaagcac cacctgcttc tgccattcgg
     5881 ggtcgagctg gaaggattcg tcggccatga gcccgggctg gtcgccggga tcctgacctt
     5941 gcgcgaaagt ttgcgaggcc gaaagcatca ggccgactgc ggccatcgac atccagatca
     6001 agcgacgaag tttcgtcatt gcaaaacgct cctcgtaggc gcggggccgg cttgtaacat
     6061 tgtttggcaa aacagtggaa ccatccattg gagagcgagc ttcatggcaa gttcatggcg
     6121 ggctcggaat gctttcgttt tccgtcactt aacgctcgtg cgggctcatt tcggcgcaag
     6181 ccacagatgc aaaagaagcg agaggagggc caccgtgccg acgcctgcaa gccacaggcc
     6241 tgcgaaccat agcaggcgca gccaaagcgg ccgcagcgtc ggctcgcccg gcatcagtga
     6301 tagccgcttc cggcccggac cttgccccgg aacacccaat aggcccaacc ggtataggcg
     6361 aggatcagcg ggatcagcac ggccacgccg aacagcatga agatctggct gtttgctggg
     6421 gatgccgcct gccagatgtc gatgctctgc ggcacgatat agggatacat gctgatgccg
     6481 agcccgagat aggacagcgc aaacaacaga agcgtcagaa agaagggtct cgtgtcctgc
     6541 ttgtcggcaa ggctgcgcaa gagggccacc gtgacgactg cgaccgcgat gggcaccggt
     6601 gcggtgagga cgatgttcgg ccaggtgaac cagcgctcgg catattgaat atgcaggaag
     6661 ggcgtcgcga tgctgaccgc gccgatcgca agcagcatcg cgaacaacag ataccaggcg
     6721 agatgatagg cgcgatcgcg caacggcccc tcggtcttca gcacgagcca ggttgcgccg
     6781 agcagcgcgt agccgatcgc aagcgagacg ccggtaagga tcgagaatgg cgtgagccag
     6841 tcccaccagc cgccggcata atggcgttcc tcgacatgca cgccctgcag gatagcgccg
     6901 agcgcgacgc cttgcgccag cgtcgccagc cacgagccgc cggcaaaggc gaggtcccat
     6961 cggttgcgtt cggatttgac gcgccagcgg aattcgaagg cgacgccacg gaagatcaac
     7021 ccgatcagca tggcgatcac aggcgtatag agcgccggca tcaagaccgc ataggcgagc
     7081 ggaaacgcgg ccatcatgcc gccgccgccg agcacgagcc aggtctcgtt gccgtcccag
     7141 accggcgcga cggtgttcat gatggtgtcg cgatcgcctt tcgcggggaa tagcggaaag
     7201 agaatgccga ggccgaggtc aaatccgtcc atcaccacat agacgaacac cgcaaaggcg
     7261 atgatgaaag cccagacggt ggcgatgtcg attgcggccg tcatcgcgcg acgctctcga
     7321 tcgcaggcgt aatgcccgcg gcgcgggccg ggatgtccct gggcagacct gcttcgcccg
     7381 gatgcggcgg aagcgccatc agccgaagca gataaatgag gcccgcgata aagaccacga
     7441 aatacacaat ggcgaacgaa atcagcgatg cgccgactgc aggcgcggcc agcggcgaag
     7501 cggaatcggc ggtccgcaac agcccgtaca ccgtgaatgg ctgccggccg gtctcggtgg
     7561 tgatccagcc tgcgagcacg gcgacaaagc cggccggtcc catcacaatt gcgaacaaat
     7621 gcaggaggcg ccattcatag agcgtgccgc gccagcgcgc ccacaggctc aacaggccga
     7681 ggccgaacat caaaaatccc atgccgacca tgatgcggaa cgaccagaac gtgatcggca
     7741 ccgggggcca gttttcacgc ggcaccgtat tgaggccttt catcggcgcg ttcaaatcat
     7801 gctcgaggat caaagacccc atcctcggaa tctcgatcgc gtatttgacc ttgccggcct
     7861 tctcgtcggg caggccgaac aggtagagcg gcgcgccgtc ggggtggctt tcgaaatggc
     7921 cctccatgcc catgatcttc acgggctgat acttcagcgt attgagcccg tgctggtcgc
     7981 cggcgatgag ctggatcggg gccaccagcg tcgccatcca catcgccatg gagaacatca
     8041 cccgtgcacc cgcaaggtgc cggtcgcgcc agagatgaaa ggcgccagcg gcgccgacca
     8101 ccagcgccgt cgtgaggtaa gccgccagaa ccatgtgaac caggcgatag ggaaaggacg
     8161 gattgaaaat caccttcagc cagtcggcgg cgacgaattg gccggcagga ttgacggcgt
     8221 agccggccgg cgtctgcatc cacgaattgg cggagagaat ccagaaagcc gagaccagcg
     8281 tgccgattgc gaccatcaac gtggcgacga agtgcagcct gcggccgacc cgctcgaggc
     8341 cgaacagcat cacgccgagg aagcccgctt cgaggaaaaa ggcggtcagg acctcatagg
     8401 ccatcaaggg accgatcacg ggtcccgtct tgtcggcaaa ggccgaccag ttggtgccga
     8461 actggtagga catcacgatc ccggagacca cgcccatgcc gaacgcgacc gcgaaaatct
     8521 tgagccagta gttgaagagg ttcaggaaaa cctcgcgccc ggtccagagc cacagcgctt
     8581 ccagcaccgc gaggtagctg gcgagcccga tcgagaaggc gggaaagacg atgtgaaacg
     8641 acatcgtgaa cgcgaattgc gcacgggcga gactgacggc ttcgaagccc gagatcagcc
     8701 aggacatcgc agacgacatt ggcgccatcc gtcattgctc gttccgaccc ggtgtagcac
     8761 gggcgatatg acgatggtat cggcccatga tccggaaaag tggatgtgtg agcaaaatca
     8821 aggggtgcgg tggctgaagc catcgtcccc ttggcccaaa cacagagcga cccggaaggg
     8881 gggcatcccg tccgggtcgt tggaggtact tgggaggttt tgaaaccgtc gcctaaatct
     8941 gtagcaaacg aagttttccg cgcgatttcg ccgattgttt caattgtttc cttgccctca
     9001 atgctttggc agagcgctcg gtccggagcg cgctgccaag tatttgaatg gattcggttt
     9061 tcaggctgcg atgctgtcca cgccggcgcc cttgagcagg tcggcgagct gcttgcgggc
     9121 atagaacatg cgggtcttca ccgtcgactg gggaatgccg atgatctcac cgacctcctc
     9181 caccgacttc tcgtgatagt agacgagatt gatgatctcg cggtgtgccg gcgacagctt
     9241 tgccacgcag gcgcgcagga tttcgcttgt ggtcgaacgg tcgagcgacg cctccggggt
     9301 gtcggcttcg tcggcgatct cgcgcacgtc gtcctggtcg atgtcctcaa accgccgctg
     9361 gcgcagcgag gtcagcgcct tgaaacgggc aatcgacaac agccaggtgg acacctggga
     9421 gcggccttcg aactggctgg cagtccgcca cacgtcgaga aacacctggc tgacgaggtc
     9481 ttcggccatc gtggtgtcgc gcacgatgcg caggatgaag cgataaaccc gcacattgtg
     9541 ccgcgcataa aggatgtgca tcgcggtctt ggagccgtcg gcaatgcttt ccagaagcat
     9601 ttcgtcggag gtcgcctggg cagcgatgat gctctggcgg gctgcggcgt tgatggcgac
     9661 gacgttctgc atgacagtct cccggggtcg ccgcttgctg cggcgctttc gttaccggaa
     9721 aacgttaatg aggctaggtt tcgggacttc tgcacgaaaa ccggaaaatg gtttcattgc
     9781 agaggagaat tgtttcgtcg cgggcccggc gacgaaacac tcagggcaga aatgtgaata
     9841 ttttcaagcg gcggaaaatg cgcggaacat tgcgtggacg caacctgacg tcgaatccgg
     9901 gcccctgaaa ggccggaaag tcagcaataa gcgccggtta ccagcctttt cgcgttcgcc
     9961 gtggggcgcg tctcaggcct tctcgccggt gggcgagaac aaatagccgc cgccgcggat
    10021 ggtacggatg accgcgggtt tcgtgggatc gggctcgatc ttgcggcgga tgcgcatgat
    10081 gcgcaggtcg accgcgcgat cgaaggcctc gctgtcgcgc gcattggcaa gttcgagcag
    10141 ccgctcgcgc gagagcacgc gcttcgggtt ggcggcaaag accttgagca ggccgaactc
    10201 ggacgcggtc agcggatgct cgttgccctc atcgtcgcgc agcgcctgcg cttcgaggtc
    10261 gagccatttg gtgccgaagc gcacgagctg gtccttggcg gttttcgcgc ttgctgcctc
    10321 ggaggcggcc ggcgccgcct tggcaggagc gctccggcgc agcaccgagc gaatgcgcgc
    10381 catcaattcg cgcagctcgc agggctttgc gacatagtcg tcggcgccaa gctcaagccc
    10441 gaccacgcgg tcgatcgggc ttgcagttgc cgtgagcatg atgacgggga cgttggtgcg
    10501 gctcttgagg tcgcggatga tggacagccc gtcttcctcg ggcatgttga gatcgagcac
    10561 aaccagatcg gggggtgcac ccgtctcgat cgcgctgcgc aggcttttcc ccccgtcgca
    10621 cagcgtaacc gtaaagccgt gcatcttgag ataatcgccg accatctccc gggccggcgc
    10681 ctcgtcatcg acaatgatga tgtgctggct ctggctcatg attactcggt cgcgggtaag
    10741 aggatggtga aggtcgatcc gaggcccgga ccgtcgctgc gggccgtcac ctcgccgcca
    10801 tgcatgtcga taatacgctt cacgatcgac aagcccagtc ccgtcgagct ctcgccggcg
    10861 gtgggcttgg ctgacagccg ctggaaccgg ccgaacagcc gcccgaggtc ttccggcgag
    10921 aggccctgac cctggtcggc gactcgaatg acggtgttgt cgtcctcgcg cgcgaccgtg
    10981 accgagatct tgccgccgat cggcgaatat ttgatggcat tgctgaacag gttgtcgatc
    11041 gcttcgcgga tgcggtcggt gtcgcacacg gtcacgaaat tggacggcgc cgacaccgag
    11101 atggtctgct gcttgttgac cgcgagcggc tggttggctt cggcaacctc gccgaccaga
    11161 gccgcaatat cgacgggctc gcggcggatg gtgatatcga acgcgtcggc catcgcgtcc
    11221 gaaatcaggt gatcgaccat ggtggtcagc cgcctggtgg cgtcgcggat gtggtcgatc
    11281 tgggccgtga tgctctcctt cgacgcgctg gtggtgatca gttcggtcag catctcggtg
    11341 cggccgagga tgacgccgag cgggttcttc aggtcgtgcg cgacggtgcc gaggatttcg
    11401 ttcttgaacc cgttggcgcg ctgcagtctc agccattgcg ccgacagacg ccggttcgcc
    11461 tgcatcagcg cgcgggtgcg ctgggcgacg cggtcctcga gctgggtgtt ggcctcgtgg
    11521 agctgctggt agaggatgac gttgtcgaac gcgatcgaga gccggctgcc gaaaatctcc
    11581 acaagcgagc ggtcggtctc ggacaattga cgctccgcct gcagcagcac cacgacctcg
    11641 cggccgctgc cggtgcggac ataaagcacg gtgcggtggt cgtggaattc gtgcttgcgg
    11701 cgcttgaacg ccgcttcgac catcgtgcgc aaatccgggt cgagcgattt cgatccggca
    11761 gatccgatga agcggctata acagccggag ccggccagca cggaaaactc gtcgttcgcc
    11821 ttgcggccgt cgtcgcgcaa aaccaggatg ccggcgcagt cgacgttgag cagggaggcg
    11881 agctgtgtca gcacgccttc ggcgagccgc tgcatcgagc ggaaatcgta tagcgtagag
    11941 gccgcatcga tgatgatttc gagaccccgc cgggtctgca ccatgcgctc gagctgctgg
    12001 tacgagcgca acgccgcggt cagcgaggta aacagcttgt cggcggtgag ttcggtcttc
    12061 gccttgtagt cgttgatgtc gtattgcacg atgacgcgcc gctccggcgc ctgtccgggc
    12121 tgcccggtgc gcaggatgat gcgaacggtc tcgttccgga tctcgttgcg gatgtattcc
    12181 acgagctcga ggccggcgac gtcggtttcc atgatgacgt cgaggagcac ggcagcgatg
    12241 tcgggatgag cgcgcatcaa ggcgcggccc tccgccgccg aataggccga caggatttcg
    12301 agcccctggc cgttcaggct gtaatcgctg agcgcgaagc gcgtgccctc atgaacggca
    12361 tgatcgtcgt cgatgacggc gatcttccat ttgcgcgccg gcgcctcctc gcgcgccgcg
    12421 tcggtatcgt cgatcaggtg gaggacatcg tcctgttcgg ccattctgcg cttccgtcgg
    12481 ctaccatatc ggatgtcccg cccttggcgg ttctgggcat aataatgcga aatgtggtgc
    12541 cttgtcccgg ccttgactcc agcatcatcc gtccgcccag ttgctgcgtg acgaggttgt
    12601 agacgatatg caggccgagc ccggtgccgc cctcgttgcg gcgggtcgta aagaagggat
    12661 cgaaggcctg gcgttgcacg tccggcgtca tgccgacgcc gttgtctgca aaattgatct
    12721 cgacgtcgtc gtggccgcgc gcccgcgccg aaatcgtgat tgttccggaa cgcccgtcgg
    12781 cgaacgcatg gttgacggcg ttgaggaaga gattggtcag gatctggccg taggaacccg
    12841 gatagccgtt gatgatcagc ccctccggca catccacgtt gagcgtgatc ggggcccgct
    12901 tcaggaccgg tcgcaagctt gcgacgatct ggtcggtcgc ctcgcctaac gaaaactgcc
    12961 ggcgttccgc gtgcgaacgg tcgaccgcga cctgcttgaa ggactgaatc aactcgccgg
    13021 cgcgatgcag gttcgccacg agctgctggg aggcgtcgcg cgaggttcgc acgaactcct
    13081 ccagttgcga gcggcgcaga ccgccttccg acctcagctc ggcctcgaac atttcggtgc
    13141 gcctcgcaag gctcgaggcc accgtcaggc tgatgccgat cgggttgttg acctcatggg
    13201 cgacgccggc gacgagaccg ccgagcgccg ccagacgttc ggcgtcgatc aggttttgct
    13261 gggcggtgtt gagctcgaac aatgcgctct cggccttctc cttcgagctg cgcaactcgt
    13321 cttccgtccg ccgtttggcg atcgcgttct cgcggaacac ctccaccgcg cgcgccatgg
    13381 cgccaacctc gtcgcgggcg gtggtgccca gcacctcgcg atcgtagtcg ccgagcgtga
    13441 tcgcgcgcat cgcggtcatg atctgctgca agggcaggcg gatggagagc gcgatcagca
    13501 cgccggcggt caggatgatg ccgaggaaga tcacggcaat cgacagcacg cggcgcgaga
    13561 tctcggacaa cgtccggtcg aaggtttcct gggccttttg ctcgcgctcg cgcatcttgg
    13621 tggagagttc gtcgatcgcg ccgatttctt cggcctgggt ggcatcgatc gtgttgcgca
    13681 acagctcggt ccgggtcgag agctgttcgg tcagctttgc caggccctcg cgcagcgccg
    13741 ccgttcgcgt attcagacgt tgcagcgcaa gccgctgcag gtcgttgtcg gcaaggtcgc
    13801 tcatcacggg gatcgtcttc tcgatcgtct cggtgttgcg acgggcttcc tcggcagaaa
    13861 gggtggcgag cgacagataa taggagttag cggcgacgag catcgcggtg aaggcctcgc
    13921 gggacttgcc gagcgagggc cagatcaggg catcgcgatg gccggtcgcc ccttcgatga
    13981 tcgagtacag cccggccatg tcgcgtgccg gcttcagaac ctgctcttca taggtcttag
    14041 cgatggtcgc ctgcaggctg cgcaattcgc cgaagccgtt gagaaagcgg tcggtgacac
    14101 gctcgagttc ctccacggac cccgccagca tcggatcggt cgaggcgcgc gtggtcagcg
    14161 tgcccagcac cgcctcgcgc agcaggagga tctcggcaaa caattcggga ctcggttgat
    14221 tgatgtagcg gtgaatcagg ttctgcagcc ggctggtttc gctttcgagc cgcgccagga
    14281 tcttgtcgga ctcgcggacc tgccgcacat cctcccacgc cgagcccagc accttggcgc
    14341 cgttccagat cagcgcggcg agcacgatga cgaccgcgga attgagcgcc gcgatcgaca
    14401 ggatgcgcca gcggatcggg actgcacgga gcagaccgac gatgcgcacg cgggccagtg
    14461 cagcaaggct cgccggccgg tccgtcgtct cgctttgctc gcgcgtggcc aaggctccgt
    14521 tcactccgcg ttgcgaatgc gatcgatcag attcgccgtc gcgggatcgc gccgggcttt
    14581 ttcgtaaacg accgacatcg ccgctatgaa cggctcgcgg tccaaatcct tcacgatctc
    14641 gacgccggcc gcttcagcct gttgccggga acgttcttcg aggtctctcc atttttcccg
    14701 catgaactgg ctcgagcgca gcgcggcttc acgaaagata ttgcgatcgt catccgacag
    14761 gctctgccag gccttcagcg acatcacgag cacttccggg gtcatcgtgt gttcggtgat
    14821 ggtgtagtag cccgcatatt tgtaatgatc ggttgtgacg aaagaaggcc agttgttctc
    14881 agccccgtcg atcagcctgg tggcaaggcc ggtgaggacc tggccatagg gcatctggac
    14941 gggttccgcg ccgaacgccc tgatcatctc cgccatcagc tccgattgtt gcacacggat
    15001 gcgcaggccc ttgaggtcgg cgagcgagcg aatcggccgg acgctgttgt agatcgatcg
    15061 cgcgccggaa tcatagaagg cgagaccgac gaatccgtag ggctcgaagc tgttgaggat
    15121 ttcatttccg atcgggccgt caagcacctt ttgcagatgc tcgatggatc ggaacaggaa
    15181 cggcatcgcc agcacattcg tcgccgggac gaaagttccg accagcgcca cgttggtgcg
    15241 gttgagatcg atggcgccga cgcgcgtctg ctcgatggtt tccttttctt cgccgagctg
    15301 gcgcgaatgg aacacgcgta tctgcagcct gccgcccgaa cgttcctcga tgaggcggcc
    15361 catatagcga agcgcctgaa cggtcggata gtcttcgctt tgagtgtcgg cggcgcgaaa
    15421 ttcgcgcgcg aaaacgcccg tggtcgcgac tgcacaaagc aacgcgacaa tcacggctaa
    15481 ggcccgcgga aggtcggcgc ggtacacagg caacttcttc cccttaattg atgatgtctg
    15541 tggtcagcgg aaaaggttca acgctttttt tgcagaaccg ggcgggatct ggctattgtg
    15601 ctgacgtcgc gatgatcgtc aatggtgcga atgagacccg tacttttgcg aaatggaaca
    15661 gcaacgggct aatcccttcg cgcgacggga ggcagggcga aaacagcagt caatggcctt
    15721 cgtgccatag atagagaatg gtttatacgg tgaaattggc actcagggtt ctgcaacttt
    15781 tggttgtttt gcttgcgatc ggcgtcgttt cgccggcgtt tgcggtcacc gacatcatgt
    15841 ggtggcatgc gatgtcgggc cagctcggca ggcaggttga taagctggcc gccgatttca
    15901 atgcctctca atccgaatat cggatcgttg cgacctacaa aggcaactac accgagaccg
    15961 tcaccgcggc gatctttgcg ttccgttcac gcagccagcc cgccatcgtt caggtcaacg
    16021 agattgcgac tgcgaccatg atggctgcga agggcgcgat ctatccggtc tacgacctga
    16081 tgcgggatgc gcaggagccg ttttcgccga ccgcctatct gccggccgtg accggttact
    16141 atgccgatgc cggcggcaac ctgctgtcgt ttccgttcaa cgcctcgacg ccgatcctct
    16201 actacaacaa gaccatgttc cgcgctgcgg gtctcgatcc cgaaagccca ccaaagacct
    16261 ggccggaact tggtcaggcc gcaaagcgcc tgcgcgagca cggcgcggcc tgcggcttca
    16321 cgacgtcatg gccgtcctgg atcaatgtcg aaaatttttc agcattccac aaccttccga
    16381 tcgcgacgcg ggccaacggc ctcgacggac tggacgccaa gctgaccttc aacaatccga
    16441 ccgtggtgcg ccatgtcgcg caacttgcgg agtggcagac cacgaaagtg ttcgactaca
    16501 gcggccgcgc gcagtccgcc gagccgcgat tccagaacgg cgaatgcggc atcttcatcg
    16561 gctcctcggc gacgcgcgcc gacatcaagg ccaattcgaa attcgaggtc ggttacggca
    16621 tgatgccgta ttggcccgac gtcgccggag cgccgcagaa caccatcatc ggcggcgcga
    16681 cgctgtgggt gctgagggat cggccgcgcg ccgaatacag aggagtcgca aaattctttg
    16741 ccttcctgtc gaagccggaa gtgcaggcgg cctggcatca gaacacgggc tacttgccga
    16801 tcacccgggc tgccttcgaa ctatcccgct cgcagggctt ttatgatcga aatccgggag
    16861 ccgcgatctc gatcgagcag atcaccttga aaccgccgac cgaaaattcg cgcgggctca
    16921 ggctcggctc gttcgtgctg atccgcgacg cgatcgagga agaattggag caggcgtttg
    16981 ccggcaagaa atcggcgcag gccgcgatgg atgccgcggt cgagcgaggc aataaattac
    17041 tgcgccagtt cgaacgggcg agcccggatc gctaggcacg attggcccac gcgtttgcgc
    17101 ggggcgaatc cgcgcttttc ccgtcgcacg ctctgcccac agaataggca gggctgtatc
    17161 tttgtatgca atgccgattg gcgccatcgg ccggtcgatc cgcgatattt ggaaagccga
    17221 tcatgggctt aagcccccgt taggggcgcg ttaacgcttg gcacaaacct tgcgacgacg
    17281 gctccaaagc ccgtttccat gttgcgttgg agcctgccat gaagcgccgc gacttcctca
    17341 aatccgtctc cggattggcc gcgggcgccc tggtgcctgc cccggcgatc atctctcccg
    17401 ccaaggcgga cgctcgctcc gagacgctgc tcatcgtctc tgaaggtgga cccaacaatc
    17461 tcgacatcca cggcgtcggc accaacgtgc ccggctacga ggtgtcgtgg aattgctacg
    17521 accggctgat cagcca
//
LOCUS       DATLJT010001036         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3058217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001036 DATLJT010000000
VERSION     DATLJT010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VM910_23855"
     CDS             complement(<1..306)
                     /locus_tag="VM910_23855"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVI65588.1"
                     /translation="MNPPPAEIGAPVAEVETPALLIDLDAFDRNIAKMAEFARAAGVR
                     VRPHAKTHKSTAIALRQIALGAVGQCVQKVGEAEVLVRGGVKDVLVSNQVVGERKLRR
                     "
     gene            460..906
                     /locus_tag="VM910_23860"
     CDS             460..906
                     /locus_tag="VM910_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVI65589.1"
                     /translation="MSEHKVTLRWERGGAEFSYQKYPRDHTWSFDGGHTMTATAAPAY
                     LGNPANVDPEEAFVASLSSCHMLTFLAIACKQKFVLDSYEDHAVGHMEKNAEGKLAIT
                     HVELHPKITWSGDRVPNAQELDKMHHAAHENCFIANSVKTDVTVVQ"
     gene            complement(971..1951)
                     /locus_tag="VM910_23865"
     CDS             complement(971..1951)
                     /locus_tag="VM910_23865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65590.1"
                     /translation="MKLPRRKFLHLAAGAAALPSVSRAAWAQAYPSRPVRAVVAFAPG
                     GVTDTFARLMAQKLTEQFGKQVYVENIAGATGNIGTAQVAKAPPDGYTILFAFSSHVV
                     NPTLFARLPYDPINDFKPVTLAVASTTVITVNPAVPAKTVNELVELIKANPGKYSFSS
                     AGAGTQAHLAGEQFRLSLGLDLVHVPFGGGGPAVAAVVAGHTPISFGSPQAAMQHVKE
                     GTVRALAVTSKTRSQLFPDVPTMTEEGYPEIEGDSWVGILVPAATPADIVAALHRESA
                     KILAQPDMKERLATLGYDVVASTPEEFAIRIKTEIDMWAKVIRAANIKTE"
BASE COUNT          408 a          721 c          710 g          422 t
ORIGIN      
        1 ccgccgtaac ttgcgttcgc caaccacctg attgctgacc aacacgtcct tcacaccgcc
       61 gcgaacgagc acttccgctt cgccgacctt ctgcacgcat tggccgaccg cgccgagagc
      121 gatctgccgt aatgcaatcg cagtcgactt gtgggtcttg gcgtggggcc gcacgcggac
      181 gcccgcggcg cgagcgaact cggccatctt tgcgatgttg cgatcaaacg catcgagatc
      241 gatgagcagt gccggggtct cgacctcggc gacgggtgca ccaatctcgg cgggcggcgg
      301 gttcatccgt ttgcgcgtgt gcttcggccg ctaccatatg caatggccga tcggcttgtc
      361 tacgacgtgg cgccgacgcg tcgtcatccc gtgcgcaagc atgaccggcg ctttgttgcg
      421 gcaattgcgc ccgcgctcgc gcaccctata tacaaaggca tgtccgaaca caaagtgact
      481 ttgagatggg agcgcggtgg agctgagttt tcgtatcaga agtatccgcg cgatcatacc
      541 tggagcttcg acggcggcca tacgatgacg gccacggcgg cgccggccta tctcggcaat
      601 cccgccaatg tcgatcccga ggaggcgttc gtcgcttcgc tctcgagctg ccacatgctc
      661 acgttcctgg cgattgcgtg caagcagaag ttcgtgctcg actcgtacga ggatcatgcg
      721 gtcggccata tggagaagaa cgccgaaggc aagctcgcga tcactcacgt cgagctacac
      781 ccgaagatca cgtggagcgg cgatcgcgta ccaaatgcgc aagagctcga taagatgcac
      841 cacgccgctc acgagaattg tttcattgcc aactcggtca aaaccgacgt gacggtggtg
      901 cagtagcccg ccgcaatcgg ggccgatcgt tggcatgcac cgcgccgacg aggtgatcgg
      961 ccggcgcgcc ctattcggtc ttgatattgg ccgcccggat caccttggcc cacatgtcga
     1021 tctcggtctt gatgcgaatg gcgaactcct ccggcgtgct cgcgaccacg tcgtagccga
     1081 gcgtggcgag ccgttccttc atgtccggct gcgcgaggat tttcgcactc tcgcggtgca
     1141 acgccgcgac gatgtccgcc ggcgtggcgg cgggaacaag gatgcccacc cagctgtcgc
     1201 cctcgatctc agggtagccc tcctccgtca tggtcggcac gtcgggaaaa agttgcgagc
     1261 gcgtcttgct ggtgaccgca agcgcgcgca cggttccttc cttgacgtgc tgcatggccg
     1321 cctgcggcga gccgaagctg atcggcgtgt gcccggcgac caccgccgcg accgcgggcc
     1381 cgccgccgcc gaaggggacg tgaacgaggt cgaggccgag cgacagccgg aactgttcgc
     1441 cggcgagatg cgcttgcgtg cccgcccccg ccgaactgaa gctatatttg ccgggattgg
     1501 ccttgatgag ctcgacgagc tcgttcaccg tcttcgccgg cacggcgggg ttgacggtga
     1561 tcacggtggt cgacgcgacc gcgagcgtga cgggcttgaa gtcgttgatc gggtcgtacg
     1621 gcaggcgcgc gaacagcgtc ggattgacca catgcgagct gaacgcgaac aggatggtgt
     1681 agccatcggg cggcgccttc gccacctggg cggtgccgat attgccggtg gcgccggcga
     1741 tgttctcgac atagacctgc ttgccgaatt gctcggtgag cttctgcgcc atcagccgcg
     1801 cgaaggtgtc ggtcacccca cccggcgcaa aggccacgac cgcgcgcacc ggccgacttg
     1861 gataggcttg cgcccatgcg gcgcgcgaca cgctcggcag cgccgcggcg cccgcggcca
     1921 gatgcagaaa tttgcggcgg ggaagtttca tcatttgctt gaccgcaaag gtcggcgcca
     1981 attgacgcgc cggctcagta gcaagattac cctagtttcg cactgaccgg aatgcgacat
     2041 gtctactccc ggcgctaaac gcacatatcg aggcaacgcg cgcatgtttt ccgcacaaag
     2101 tatccaaacc ataagctcaa catcacaaat cgacgatcgt tctcggcggg acttccggtg
     2161 gtttgggagc ctttcctagc gtcactagac gctgcagcca agttagctcg cgctcaggca
     2221 tcttcggagc cgctacatag ctgatcacga gatggttgtt c
//
LOCUS       DATLJT010001037         4302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2138831,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001037 DATLJT010000000
VERSION     DATLJT010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138831"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..947
                     /locus_tag="VM910_23870"
     CDS             108..947
                     /locus_tag="VM910_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI65591.1"
                     /translation="MNFRVLPASVGPRGRLLALLATTALAVSFAVDAQAQTPAPAAPP
                     APKKAAPKAAPKAPAAPAPAAQAPAQPPAAAQAQQPPANGQAQPQDQQVQLIYAPWTK
                     FCLKGQDANAKQVCFTGKDGRIESGQPVVAAVIIEPEGEPKKILRVTLPLGMQLVHGT
                     RVIIDNNQPQQSPYVICFANGCMSDYEATPELLANMKKGQNLVVQAINANGAPLTLPL
                     PLQESGGSSFAKAYDGPPTDPKVFEENQKKLQEELQKRADEARKKLEATQGAGASPNP
                     AAK"
     gene            complement(1052..1384)
                     /gene="hspQ"
                     /locus_tag="VM910_23875"
     CDS             complement(1052..1384)
                     /gene="hspQ"
                     /locus_tag="VM910_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HVI65592.1"
                     /translation="MIIARTAKFQIGQIVRHRIFSFRGVIFDIDPEFNNTEEWWLSIP
                     EEVRPHKDQPFYHLLAENSETEYVAYVSEQNLLPDDSGEPIRHSQVAEIFVKDKSGGY
                     RPRNPSLN"
     gene            complement(1582..2787)
                     /locus_tag="VM910_23880"
     CDS             complement(1582..2787)
                     /locus_tag="VM910_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HVI65593.1"
                     /translation="MANSSVKYDVAVVGGGPAGFTTAIALAQYGAKTALIARRAPYAD
                     NRTTALLGASIDLLQRLNVWQRCEDKAAALKTMRLVDDTGRLIRAPEVRFSSEEIGLE
                     TFGYNIENRDLITALEERAAELANLTRLDDEAQSVTPDDAAVTIRTAGGCSVAACLAV
                     GADGRHSLCREAAGIDVTRHDLAQAALTFNIAHTRSHRNASTEFHTALGPCVFVPLPG
                     DRCSVVWVASSREAERLYALSDDELSDAAERQAHSILGRVRVEHERHLFPLAIERPIR
                     FAQKRIVLVGEAAHVVPPIGAQGLNMGLRDAADIADIVRERVAGGDDAGAPQVARRYD
                     MARRPDVLSRTFVIDLANRSLLSDFLPMQSARVAGLHLIGAIGPLRRLAMREGLAPSW
                     RRQRSGRDS"
     gene            3026..3760
                     /locus_tag="VM910_23885"
     CDS             3026..3760
                     /locus_tag="VM910_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVI65594.1"
                     /translation="MEQFEHKDSSLAKPSKRAAAFSVHVFTALGAGVALLALLEAVRE
                     HWAAMFAWLGVALIIDAADGPMARRLDVVRLQPHWSGDVLDLVVDFVTYVFVPAYAIT
                     ASRLLLPVAAPVLGIGIAVSGALYFADRRMKSADNHFRGFPGLWNVAAFYLFLLHWPP
                     ALSSLAVLALIVATFLPFHVVHPVRVKRLRWLTLTLMVAWGILAIYTLAKDFDVEFPI
                     TAALCLIAVYIVASDAAIRFLRSLKA"
     gene            3757..>4302
                     /locus_tag="VM910_23890"
     CDS             3757..>4302
                     /locus_tag="VM910_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVI65595.1"
                     /translation="MIELLTSAESWAALLTLTALEIVLGIDNVIFLSVIVSRIPQRQA
                     KRARQIGLALALMFRILLLSLLVWLIGLTQDLFSVRGVSFSWRDIILIGGGLFLIGKA
                     THEIHSEVEGDAEEAERASGTTAFLWVIVQIIVIDMVFSLDSIITAIGMVQDLEIMIA
                     AVVIACAIMYISSGPVSRFVAE"
BASE COUNT          775 a         1356 c         1341 g          830 t
ORIGIN      
        1 cctgtcacgt atccgcctca attgactagg agacagccca cgaaacggcg ttgacggcgc
       61 tatcgggcgg ttttcgggcg ctccccgttc agaaagggtt ttccgcgatg aatttccgtg
      121 ttttgcccgc gtcggtcggg ccgcgtgggc ggctcctcgc cttgttggcg accaccgcgc
      181 tggctgtttc gtttgccgtt gacgcccagg cccagactcc cgcgccggct gccccgccgg
      241 cgcccaagaa agcggcccct aaggctgctc ctaaggcgcc ggccgcgcca gctccggccg
      301 cccaggcacc ggcccagccg ccggccgccg ctcaggctca gcagccgccc gccaacggtc
      361 aggcgcaacc ccaggaccag caggtgcaac tgatctatgc gccctggacc aagttctgcc
      421 tcaagggcca ggatgccaac gccaagcagg tctgctttac cgggaaggac ggtcgcatcg
      481 agtctggcca accggtggtg gccgccgtca tcatcgagcc cgagggcgag ccgaagaaga
      541 tccttcgcgt cacgctgccg cttggcatgc aactcgttca cggaacccgc gtgatcatcg
      601 acaacaacca gccgcagcag tcgccctacg tcatctgctt cgccaatggg tgcatgtccg
      661 actacgaggc aacaccggag ctcctggcca acatgaaaaa gggccagaat ctcgtggttc
      721 aggccatcaa cgccaacggc gcgccgctaa cgctgccgct gccgctgcag gagtcgggcg
      781 gaagcagctt cgccaaggcg tatgacggac cgccgactga tccgaaggta ttcgaggaaa
      841 atcagaagaa gctccaggaa gagctgcaga agcgcgcgga cgaagcgcgc aagaaactcg
      901 aggccacgca aggggcgggg gcttcgccga atccagccgc gaaataatcc gccggcttgg
      961 ccggaacaca aaaggcgctt cctgaggaag cgcctttttc gttacggcga ccgcgcggcc
     1021 tttgcgtcgc atcccgacgc gggacggcgg gtcagttcaa ggacggattt cgcggacgat
     1081 agccgcccga tttgtctttc acgaaaatct cggcgacctg ggaatggcgg atcggctccc
     1141 cggaatcgtc aggcagcaga ttctgctcgg agacataggc gacgtattca gtctccgagt
     1201 tctcggcgag cagatgatag aacggctgat ccttgtgagg gcggacctcc tcggggatcg
     1261 acagccacca ctcctcggtg ttgttgaact ccggatcgat gtcaaagatc acgccgcgga
     1321 acgaaaagat ccggtggcga acgatctgcc cgatctggaa cttggcggtt ctcgctatga
     1381 tcataccaac cgaatagacc agtctcgggt ctcgcgctag tgcttcgagc tgtggattaa
     1441 cccttgttaa gccgacgcca agcgcctgcg acctcaaaat cccgtcaaat gatcgacatt
     1501 ctcaagctcg ccgtgccgca tttcggcctg atcttttttg ccttgcgtgc ggcaaggcgc
     1561 gagaccggct gaagcgtttc ctcaggaatc cctacccgag cgctgcctgc gccaggacgg
     1621 cgccagtcct tcgcgcatgg cgaggcgccg caagggcccg atcgcgccga tcaggtgtaa
     1681 tcctgccacc cgcgcagatt gcatgggcag gaagtcgctg agcagggaac ggttggcgag
     1741 atcgatgaca aaggtgcggc tgagcacatc agggcgccgc gccatgtcgt agcggcgggc
     1801 tacctgtggg gcgcccgcat catcgccgcc tgcgaccctc tcgcgcacga tgtcggcgat
     1861 atcggcggcg tcgcgcaatc ccatgttcaa gccctgcgcg ccgatcggcg ggaccacatg
     1921 cgccgcctcg ccaaccagca cgatgcgttt ttgagcaaaa cgtatcggcc gctcgatagc
     1981 gagcggaaac agatgccgct cgtgctcgac ccggacgcga ccgaggatcg aatgcgcttg
     2041 tcgttcggct gcgtcggaca attcgtcgtc ggaaagcgca taaagacgct cggcctccct
     2101 tgaactggcg acccaaacga cgctgcagcg atcgccgggg aggggaacga agacgcaggg
     2161 tcccaacgca gtgtggaatt cggtggaggc gttccggtgc gaccgggtat gagcgatatt
     2221 gaatgtcagc gccgcctggg cgaggtcgtg cctcgtgaca tcgataccgg cggcttcgcg
     2281 gcacagcgaa tgcctcccat ccgcgcccac ggcgaggcag gcggcgacgg aacatccgcc
     2341 ggccgtgcgg atcgtgaccg cggcatcgtc gggtgtgacg gattgggcct catcgtcgag
     2401 gcgcgtcaga ttggcaagct cggccgctcg ctcctccagc gcggtgatca ggtcgcgatt
     2461 ttcgatgttg taaccgaagg tctcgaggcc gatttcctcc gaggagaacc gcacctccgg
     2521 tgcgcggatc agacgcccgg tatcgtcgac cagccgcatc gtcttcagtg ccgcggcctt
     2581 gtcctcacag cgttgccaaa cattgagccg ctgcaggagg tcaatcgacg ctccgagcag
     2641 agcggtggtc cggttgtcgg cataaggcgc gcggcgcgca atgagcgcgg tcttcgcgcc
     2701 gtattgcgcg agggcgatcg ccgtcgtgaa gccggcgggt ccgccgccca ccacggcgac
     2761 gtcatatttg acggaactat tggccatcat ggtccacaat tggctccggc ctgcagcaat
     2821 ttcaagccag cagagggcgc acgcgcggcc gaacgccgca acctcgcatg tacacatgaa
     2881 gcctattttt ggctagtgga gcgaatttga cgttcgcacc gtctcgatgc gattgctcaa
     2941 tgcgaacgtg attccaacat tcgcatatgt ggctcgccga atgcgatgcg aatgttagaa
     3001 tcggatcact gacaccgcca cgcccatgga acagtttgaa cataaggatt cgtcgctcgc
     3061 caagccgtcg aagcgggctg ccgcattttc cgtgcatgtc ttcacggctc tcggggccgg
     3121 ggttgcgctg ctggcactcc tggaagccgt acgcgagcat tgggccgcga tgttcgcctg
     3181 gctcggagtt gcgctcatca tcgacgccgc ggacgggccg atggcgcgac ggctggacgt
     3241 cgttcgcctg cagccgcact ggtcaggtga cgtgctcgat ctcgtggtcg atttcgtcac
     3301 ctatgtattc gtgcccgcct atgccatcac cgcaagccga ttgctgctgc cggtggccgc
     3361 acccgtgctc ggcatcggga tcgctgtcag cggcgcgctg tatttcgccg atcgccggat
     3421 gaagagcgca gacaatcatt tccgcggttt tcccggactt tggaatgttg cggcgttcta
     3481 tctgttcctg ctgcattggc cgccggcgct ctcgagcctc gccgtacttg ccttgatcgt
     3541 cgcgaccttc ctaccctttc acgtcgtgca tcccgtgcgc gtgaagcggt tgcgctggct
     3601 gacgctgacg ttgatggtcg cgtggggcat tctcgcgatc tatacgctgg cgaaggattt
     3661 cgatgttgaa tttccaatca ccgcggcact gtgcctgatc gccgtctaca tcgtggccag
     3721 cgatgcggcg atccgtttcc tgcggtcgct gaaggcatga tcgagcttct caccagcgcc
     3781 gaatcctggg cagccctgtt gacgttgacg gcgctggaaa tcgttctcgg catcgacaac
     3841 gtcatcttcc tgtcggtcat cgtctcgcgc attccgcagc ggcaggccaa gcgtgcgcgc
     3901 cagatcggtc ttgccctggc gctgatgttt cgcatcctct tgctgagcct tctggtgtgg
     3961 ctgatcggtc tgacccagga cttgttcagc gtgcgcggag tttccttctc atggcgcgac
     4021 atcatcctga tcgggggagg gctgttcctg atcgggaaag ccacccacga gattcattcc
     4081 gaagtggaag gagacgcgga agaggccgag cgggcaagcg ggaccaccgc atttctctgg
     4141 gtgatcgtcc agatcatcgt catcgacatg gtgttttcgc tcgactccat catcaccgcg
     4201 atcggcatgg tgcaagacct cgaaatcatg atcgcggcgg tcgtgatcgc ctgcgcgatc
     4261 atgtacatat cctcgggccc ggtgtcgcgc ttcgtggcgg ag
//
LOCUS       DATLJT010001038         4146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2138865,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001038 DATLJT010000000
VERSION     DATLJT010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138865"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /gene="lptG"
                     /locus_tag="VM910_23895"
     CDS             complement(<1..992)
                     /gene="lptG"
                     /locus_tag="VM910_23895"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HVI65596.1"
                     /translation="MSMITNTLGRYFAGRFVVSALGVFASIFLLLVLVDYIEMVRRTS
                     GLVSASPIMVAETSLFRVPQLLEKMMPFCVLIGAMTCYLALSRRLELVVVRAAGVSAW
                     QFIAPALASAMVLGILATVGYNPMSANLRELSKRMEAELFGGAPGGGVQDAAGFWINQ
                     VNADGQVIINAARSEQQGVRLTGLTLFRFDTDNQFKERIEAREGRLEEGRWLFKSIRR
                     YSLNAPPVDQDSFELPTSLTPAQVRNSFSTPETVSFWQLPSYIRSSESSGFATAGYRL
                     QYHKLVAQPFLLAAMVLLAASVSLRFFRFGGVQKMVLSGVGAGFLLYVLSKVTE"
     gene            complement(989..2155)
                     /gene="lptF"
                     /locus_tag="VM910_23900"
     CDS             complement(989..2155)
                     /gene="lptF"
                     /locus_tag="VM910_23900"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HVI65597.1"
                     /translation="MGSIDRYIFRTTLASFALVLVSLTGVIWITQALRGIDLMTSQGQ
                     TVATFLGLTSLVIPALVLVIAPIALMIAISHTLNKLATDSEIIVMNAAGLSPFRLFRP
                     FVHATIVVALLVAFIAAYLAPDGMRRIKQWDAEITADVLTNILQPGRFAQLDQNLTIR
                     IRERQPGGILLGIFIDDRRDPKERVSIVADHGTVVKSGNGSFLVLEDGNLQRFEAGKR
                     DPALVAFGRYAFDMSKFSQGHNTSLGIRERYLWELFSVDENDPMSRLLSGQFRAELHD
                     RFMAPIYPFAFAALTFAFLGTPRTTRQSRNFSIGSAILAVFGLRMAGFACSVMTVKSP
                     WAALVQYLLLAGAIVGSLWIILGSVVIEPPAAMMEAINNFNARLQRLFRRTAAA"
     gene            2495..3994
                     /locus_tag="VM910_23905"
     CDS             2495..3994
                     /locus_tag="VM910_23905"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HVI65598.1"
                     /translation="MSDAVKVGFVPLSAPARGTLVVFCDEALKLGAATRKALGGAADL
                     VKRAAAAAEFKGKNGATLDLLEPDGIKIQRLIVVGVGKPSELKEKDFLKFGGALAGKL
                     NAATEAVTVLAELPDGAIEPANAASIGAGVRLRAYRFNRYKTKKKDENDALRAQVSIL
                     VGDVAATRKAFAASAQVVDGVLMARELVNEPPNVLYPEEFARRAGQLRKLGVTVEVLD
                     VKAMTKLGMGALLGVAQGSARPGRTVIMRWNGGKRGAQPVAFVGKGVCFDTGGISIKG
                     AANMEDMKGDMGGAACVVGLMQALAARKAHVNAVGAIGLVENMPDGNAQRPGDIVTSM
                     SGQTIEIINTDAEGRLVLADLLWYVSKKFKPKFIVDLATLTGAIMVALGTEHAGLFSN
                     NDELAQRLSEAGAATGEKVWRMPLGPEYDKLIDSQFADMKNTGGRHGGSITAAQFLQR
                     FVDSTPWAHLDIAGTAMGAPKTEINQSWGSGYGVRLLERLVANHYEALK"
     gene            4007..>4146
                     /locus_tag="VM910_23910"
     CDS             4007..>4146
                     /locus_tag="VM910_23910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HVI65599.1"
                     /translation="MTDVLFYHLQNMSPESVLPPLLEKSLERGWRVVVQATSQERADA
                     LDA"
BASE COUNT          865 a         1247 c         1331 g          703 t
ORIGIN      
        1 tcctcggtaa ctttggacag aacatagagc agaaagcccg cgcccacgcc actcaaaacc
       61 atcttttgta cgccgccgaa tcggaagaag cgcaagctga cggatgcagc cagcaagacc
      121 atcgcggcca gcaaaaacgg ctgcgcgaca agcttgtggt actgcagccg atagccggcc
      181 gtcgcaaatc cggagctttc ggacgaacga atgtagctcg gcagttgcca aaaagagaca
      241 gtttccggcg tcgagaagct gttgcgtacc tgcgccggcg tcaggctggt gggcagttcg
      301 aaactgtcct gatcgaccgg cggcgcattg agcgaatagc gccgtatcga tttgaacagc
      361 cagcggcctt cttcgagcct gccttcgcgc gcctcgattc gttccttgaa ctgattgtcc
      421 gtatcaaatc gaaacaaggt cagcccggtc aggcgcacgc cctgttgctc gctgcgggcg
      481 gcgttgatga tgacctgacc gtcggcattc acctgattga tccagaaccc cgcggcgtcc
      541 tgaacgccgc cgccaggggc gccgccgaac aactcggctt ccatccgctt ggagagttcg
      601 cggagattcg ccgacatcgg attgtagccg actgtcgcaa ggattccgag caccatggcg
      661 ctggcaagcg ccggcgcaat gaactgccac gccgagacgc cggcggcgcg tacgaccacc
      721 agttcgagcc gccgcgacaa tgcgagatag caggtcatcg cgccgatcag gacgcagaag
      781 ggcatcatct tttcgagtag ctgtggtacg cgaaacaggg aggtctccgc caccatgatc
      841 ggggaagccg aaaccaggcc tgaggttctg cgcaccattt caatgtaatc gacgagcacg
      901 agcagcagga aaatgctggc gaacacgccg agtgccgaga caacgaagcg gccggcgaaa
      961 taacgtccaa gcgtgttggt gatcatgctc atgctgcggc cgtccgtcgg aaaagccgct
     1021 gcaggcgggc attgaagttg ttgatcgctt ccatcatcgc tgccggcggt tcgatcacca
     1081 cgctgccaag gatgatccag aggctcccca cgattgcgcc cgccagcaac agatactgaa
     1141 cgagcgcggc ccagggcgat ttcaccgtca tgaccgagca ggcaaatccc gccatgcgca
     1201 atccgaacac cgccaggatc gcgctgccga tcgagaaatt ccggctctgg cgcgtggtgc
     1261 gcggggtacc gagaaaggcg aacgtcaggg ctgcgaacgc gaacggatag atcggcgcca
     1321 tgaagcggtc atgcagttcg gctcggaact ggccggaaag cagcctggac atcggatcat
     1381 tctcgtcgac cgaaaacagc tcccacaggt agcgctcgcg gataccaagc gaggtgttgt
     1441 gcccctgcga gaatttcgac atgtcgaaag cgtaacggcc gaacgccacc aatgccggat
     1501 cgcgctttcc cgcctcgaaa cgctgcaagt tgccgtcctc gagaaccagg aacgagccgt
     1561 tgccgctttt gaccacggtt ccgtggtcgg cgacgatgct cacgcgctcc ttgggatcgc
     1621 ggcggtcgtc gatgaaaatg ccgagcagta tgccgccggg ttgccgctcg cggatgcgga
     1681 tggtcaggtt ctgatcgagt tgtgcaaaac gtccgggctg cagaatgttg gtgagcacgt
     1741 cggccgtgat ctcggcgtcc cactgcttga ttcgccgcat gccgtcaggc gcgagatagg
     1801 cggcaatgaa ggccaccaac agcgccacca cgatggtcgc atgaacgaag gggcgaaaca
     1861 gccggaaggg cgagaggccg gccgcgttca tcacgatgat ctcggagtcg gtcgcgagct
     1921 tgttcagggt gtgggagatt gcgatcatca gcgcgatcgg tgcgatgacc agcacgagcg
     1981 ccgggatgac gaggctcgtg agaccgagga aggtcgcgac cgtctgaccc tggctggtca
     2041 tcaaatcgat gccgcgcaac gcctgcgtaa tccagatcac cccggtgagg ctgaccagga
     2101 cgagcgcaaa cgacgcaagc gtcgtgcgga aaatgtacct gtcgatcgac cccatcgtta
     2161 ccgcacgatc ccgccctgcc acgccaggac tgacgaactg caaccatccc tcgaaagggt
     2221 ccccaatcgg tcgcgccgcc gccccgtccc gcacgcttat tgtctcacac catctccttg
     2281 atccgtcaac aaaatggccg cggaatggca ccgcagggcg atggttgata tttcgatcca
     2341 tgtggccacc ctgtcacggc ggcgcttggc aggcgcaggg cccgcaggcc atagtgggag
     2401 aatgaggccg cctcgcatcg caaagggcgg cggaagagcc gaccaggagc cggctgctca
     2461 cgccgcaaag ccttgatttt tggaggattt atctatgtcc gacgccgtga aggtcggctt
     2521 cgtcccgctg tccgcgcccg cgcgcggcac gctggtggtg ttctgcgatg aagcgctcaa
     2581 gctcggggct gccacccgca aggcgctcgg gggcgcggcc gacctggtca agcgcgccgc
     2641 cgcagcagcg gagttcaagg ggaagaacgg ggcgaccctc gatctcctgg agcccgacgg
     2701 aatcaagatt cagcgcttga tagtggtcgg agttggcaag ccatccgagc tgaaggagaa
     2761 ggatttcttg aagttcggcg gggcattggc cggaaaactc aatgccgcga ctgaagcggt
     2821 gacggttctt gcggaattgc cggacggcgc catcgaaccg gcgaatgccg cgagtattgg
     2881 cgcgggcgtc cgactccgcg cctacaggtt caaccgctac aagaccaaga aaaaggacga
     2941 gaacgacgca ctgcgcgcac aggtttcgat cttagtcggc gatgtggccg cgacccgcaa
     3001 ggcgtttgcg gcgagtgcgc aggtggtcga cggcgtgttg atggcgcgcg aactcgtgaa
     3061 cgagccgccg aatgtgcttt accccgagga gttcgcgcgt cgggccggcc aactgcgcaa
     3121 gcttggcgtc accgttgagg ttctcgatgt caaggcgatg accaagctcg gcatgggcgc
     3181 gctgctcggg gttgcgcagg gatcggcgcg gccgggccgc accgtcatca tgcgctggaa
     3241 cggcggaaag cgcggcgctc aaccggttgc gtttgtcggc aagggcgtct gtttcgacac
     3301 gggcggtatc tcgatcaagg gcgcggcgaa tatggaggac atgaaaggcg acatgggagg
     3361 cgctgcctgc gtcgtcggct tgatgcaagc gctcgccgcg cgaaaggcgc atgtgaacgc
     3421 ggtcggcgcg atcggtctgg tcgaaaacat gcctgacggc aacgctcagc gccctggcga
     3481 catcgttacc tcgatgtcag gccagaccat cgagattatc aacaccgacg ccgagggccg
     3541 gctcgtgctg gcggatctgc tctggtacgt gtcgaagaaa ttcaagccga aattcatcgt
     3601 cgatctcgcg acgttgaccg gcgcaatcat ggtggccctt ggcactgaac atgccggcct
     3661 gttctccaac aatgacgagc tggcgcagcg cctgagcgag gcaggcgcag caaccggcga
     3721 aaaagtatgg cgcatgccgc ttggtcccga gtacgacaag ctgatcgatt cgcaattcgc
     3781 cgatatgaag aataccggcg gccgacatgg cggctcgatc acggctgcac aattcctgca
     3841 acgcttcgtc gacagcacgc cgtgggcgca tctcgatatc gcgggaactg caatgggcgc
     3901 tccgaagacg gagatcaacc agagctgggg ctccggctac ggcgttcgcc tgctagagcg
     3961 gctagtcgcc aaccactacg aagcgctgaa gtaaaccaag cggcacatga ccgacgttct
     4021 gttctatcat ctgcaaaaca tgtcgccgga gagcgtcctg ccgccgctgc ttgagaaatc
     4081 gctggagcgc ggctggcggg tcgtcgtgca ggcgacctcg caagaacgag ccgatgcgct
     4141 ggatgc
//
LOCUS       DATLJT010001039         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4437311,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001039 DATLJT010000000
VERSION     DATLJT010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4437311"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VM910_23915"
     CDS             complement(<1..1071)
                     /locus_tag="VM910_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65600.1"
                     /translation="MAGGPLLDVRDLTVEFSTRRGIVKAVQHVNISVAKGETLGIVGE
                     SGSGKSVTSYAVMRILDRAGKIAEGSVMFSGIDVKGASEEAMRDLRGREISMIFQNPR
                     AALNPIRKVGDQIEDVLRQHVMTAGIDGGEKAIEALEQVKIARPRERYHAYPFELSGG
                     MCQRVVIALALACNPQLLIADEPTTGLDVTTQKAVMDLVVELTRQRAMSTILITHDLG
                     LAAAYCDRVVVMEKGRVVETAKSADIFARPQHPYTRKLMRATPRIGVSLRDLLPEEDA
                     AASTSTVTAGLDPAIRQSSQSSSCSMDSRVKPEGDKSTKPLLLVEKLVKEYPRNGATA
                     ALSKLFGRKPPVEQEVFRAVDGI"
     gene            complement(1073..1975)
                     /locus_tag="VM910_23920"
     CDS             complement(1073..1975)
                     /locus_tag="VM910_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI65601.1"
                     /translation="MSTVAPAAEPIAQARPSSGLWATLEHTRYVLGENRVTAFAFALL
                     LVIVAAALLGPFVVPYDPLASDTAMALKPPSAAHWFGTDQLGRDIFSRVIVATRLDTF
                     IAVSSVVLVFLMGGLAGIAAGYFGSWTDRIVGRIADTIMAFPLFVLAMGIVAALGNTV
                     QNIIIATAIVNFPLYARVARAEANVRRNAGFVQAARLSGNGEFRILLGHILPNIMPIM
                     IVQMSLTMGYAILNAAGLSFIGLGVRPPTAEWGIMVAEGASFMVSGEWWIALFPGLAL
                     MIAVFCFNLLGDGLRDIVDPQRRT"
     gene            complement(1983..2996)
                     /locus_tag="VM910_23925"
     CDS             complement(1983..2996)
                     /locus_tag="VM910_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI65602.1"
                     /translation="MLTMIGKRLMFAIPSLIGVVVVTFLLTRALPGDPAAYFAGPAAT
                     KEAVEQIRKKLGFDKPLIEQFFRYTGDLVHGDFGTSLTTGQPVATEIRNRLPASAELT
                     LLGLFISVAIALPLGVLAATRPGSWIDHLCRVATTAGVSLPVFFTGLVLVYVFYYLLG
                     WSPAPLGRLDVFYSAPPTLTGFYLIDTLLARDVEAFRSALSQLVLPATTLAIFSLAPI
                     ARMTRASMLSVLASDFVRTARASGLKPSTVIVTYAFRNAMLPVITTLSMVFSFLLGAN
                     VLVEKVFAWPGIGSYAVEALISSDFAPVQGFVLTMAVMYVALNLMIDILYGVIDPRVR
                     LEG"
     gene            complement(3151..>4356)
                     /locus_tag="VM910_23930"
     CDS             complement(3151..>4356)
                     /locus_tag="VM910_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65603.1"
                     /translation="FPTFQMSAGSLTKPEQFVIVDDQTVRVDFAKKDRLTIPDLAVIV
                     PCVVNSELVKKSASEKDPWGLEYTKQQTAGSGAYKVTKWTAGTEVIMERNDQWVGGPL
                     PKIRRVIWRMVPQAGNRRALLERGDADISYELPNKDFQEMKAESKLNVISLPFSNGIQ
                     YIGMNVTRPPFDNLKVRQAVAHAIPYQKIMDAVLFGLANPMFGAPADKPTEVAWPQPT
                     KFFTDIDKAKALMAEAGFANGLETTLSFDLNFAQINEPLCVLVQESLGQIGIKTTINK
                     VPGANWRTELTKKELPLFTNVFSGWLDYPEYFFYWCYHGNNSIFNTMSYKSPEMDKLI
                     DGARTAAATGDKAAYDTDVKGFVDLAFADIPRIPLYQPYVNVAMQKNVSGYQYWFHRR
                     LDYRALVKG"
BASE COUNT          838 a         1349 c         1420 g          749 t
ORIGIN      
        1 gatgccgtcg acggcgcgga acacctcctg ctccaccggc ggcttgcggc cgaacagctt
       61 tgacagcgct gcggtcgcgc cgttgcgcgg atattccttc accagcttct cgacaagcag
      121 caggggtttt gtgctcttgt caccttccgg cttgacccgg gagtccatcg aacaagaaga
      181 gctttgcgaa gattgacgga tggccgggtc aagcccggcc gtgacggtgg aggtcgaagc
      241 ggcggcgtcc tcctccggca agagatcgcg cagggacacg ccgatccgcg gcgtagcgcg
      301 catcagcttg cgcgtatagg gatgctgggg ccgggcaaag atgtcggcgg atttcgcagt
      361 ctcgaccacg cggcctttct ccatcaccac cacgcggtcg caataggccg ccgcaagtcc
      421 gaggtcgtgc gtgatcagga tcgtggacat cgcacgctgc cgcgtcagct ccacgaccag
      481 gtccatcacc gccttctgcg tggtgacgtc gaggccggtg gtcggctcgt ccgcgatcaa
      541 aagctgcgga ttgcaggcaa gcgcaagcgc gatcaccacg cgctggcaca tgccgcccga
      601 cagctcgaac ggataagcgt gatagcgttc acgggggcgg gcgatcttga cctgctcgag
      661 cgcttcgatc gccttctcgc cgccatcgat gccggccgtc atcacgtgct ggcgcagcac
      721 gtcctcgatc tggtcgccga ccttgcggat cggattgagg gcggcgcgcg ggttctggaa
      781 gatcatcgag atttcgcggc cgcgcaggtc ccgcatcgcc tcctcgctcg cacccttgac
      841 gtcgatgccg gagaacatca cggaaccttc cgcgatcttt ccggcgcggt cgagaatacg
      901 catcacggca taggaggtaa ccgatttgcc cgaaccggac tcgccgacga tgccgagcgt
      961 ctcgcccttg gccacggaga tgttgacatg ctgcaccgcc ttgacgatgc cgcgccgggt
     1021 ggagaactcc accgtgagat cgcggacgtc gagcagcgga ccgccggcca tgtcacgtcc
     1081 tccgctgcgg gtcgacgatg tcgcgcaggc cgtcgccgag caggttgaag cagaacaccg
     1141 cgatcatcag cgcgagtccc gggaacagcg cgatccacca ttctcccgac accatgaagc
     1201 tcgcgccttc cgcgaccatg atgccccatt ccgcggtcgg cggccgcacg cccagcccga
     1261 tgaacgaaag tccggcggca ttgaggatgg catagcccat ggtcagcgac atctgcacga
     1321 tcatgatcgg catgatgttc ggcagaatgt gacccagcag aatcctgaac tcgccgttgc
     1381 ccgaaagtct cgccgcctgc acgaaaccgg cgttacgccg cacattggct tcggcgcggg
     1441 cgacgcgcgc atagagcggg aaattgacga ttgcagtcgc aatgatgatg ttctgcacgg
     1501 tgttgccgag cgcagccacg atgcccatcg ccagcacgaa cagcggaaac gccatgatgg
     1561 tgtcggcgat gcggccgacg atgcgatcgg tccagctacc gaaatacccg gcggcaattc
     1621 cggcaagccc gcccatcaga aacaccagca ccaccgagct tacggcgatg aaggtgtcga
     1681 gccgggtcgc gacgatgacg cggctgaaga tgtcgcgccc caactggtcg gtgccgaacc
     1741 agtgcgcggc cgagggcggc ttcagcgcca tcgcggtgtc ggaggccagg ggatcgtacg
     1801 ggacgacaaa tgggccgagc aacgccgccg cgacgatgac gagcaggagc gcgaatgcga
     1861 aggcggtgac gcgattctcg ccaagcacgt agcgggtgtg ctcgagcgtc gcccaaaggc
     1921 ctgagctggg acgggcttgc gcgatgggtt cggcggctgg agcgacggtg ctcatcttca
     1981 tgtcatcctt cgagccggac gcgcggatcg atcacgccat agagaatgtc gatcatcagg
     2041 ttgagcgcca catacatcac cgccatggtc agcacgaagc cctgtaccgg cgcgaagtcc
     2101 gacgagatca gggcttccac cgcataggag ccgataccgg gccatgcgaa cactttctcg
     2161 accagcacgt tggcgccgag caggaacgag aacaccatcg acagcgtggt gatcaccggc
     2221 agcatcgcgt tgcggaacgc gtaggtgacg atcacggtcg acggcttcag cccgctggcg
     2281 cgcgcggtgc gcacgaaatc ggaggccagt accgacagca tcgaggcgcg cgtcatgcgc
     2341 gcgatcggcg ccagcgagaa gatcgccagc gtggtcgccg gcagcacgag ctggctcagc
     2401 gcggaacgga aggcttcgac gtcgcgtgcg agcagcgtgt caatgaggta gaagccggtc
     2461 aatgtcggcg gcgcgctgta gaacacgtcg agccgcccga gcggtgccgg cgaccagccg
     2521 agcaggtaat aaaagacata gaccagcacg aggccggtga agaacaccgg cagcgatacg
     2581 ccggccgtgg tggcgacgcg acagaggtga tcgatccagg agccgggccg ggttgccgca
     2641 agcaccccga gcggcagtgc aatggcgacc gagataaaga ggccgagcag ggtcagttca
     2701 gccgacgccg gcaggcggtt gcggatttcg gtcgcgacgg gctgcccagt ggtcagggac
     2761 gtgccgaagt cgccgtggac gagatcgccg gtgtagcgga agaactgctc gatcagcggc
     2821 ttgtcgaaac cgagcttctt gcggatctgc tcgacggcct ctttcgtcgc ggcagggccg
     2881 gcaaaatagg ctgcgggatc gccgggcaga gcgcgggtca agaggaacgt gaccacgaca
     2941 accccgatca ggctcgggat cgcaaacatc agccgcttgc cgatcatcgt caacatgagg
     3001 ctcgctcccg caaaagtccg gggcgggagg catcagggcc ggtccagata cgccccccac
     3061 cccggctcgc atcgcagtca cgatgctgct tgatccgctc cgccgccttg cgacggagag
     3121 gaaaacgcac gacgaagatt tgctcggccc tcaccccttc accagcgcgc gatagtcgag
     3181 gcgccggtga aaccagtatt gatagcccga cacgttcttc tgcatcgcga cgttgacata
     3241 gggttgatag agcggaatgc gcgggatgtc ggcaaaggca agatcgacaa aacccttcac
     3301 gtccgtgtcg taggccgcct tgtcgccggt cgcagccgca gtgcgcgcgc cgtcgatgag
     3361 cttgtccatc tccggcgact tgtagctcat ggtgttgaag atcgaattgt tgccgtgata
     3421 gcaccagtag aagaagtatt cggggtaatc gagccaaccc gaaaaaacgt tggtgaagag
     3481 cggcagctcc ttcttggtca gttcggtgcg ccagttggcg ccgggcacct tgttgatcgt
     3541 cgtcttgatg ccgatctggc ccaggctttc ctgcaccagc acgcaaagcg gctcgttgat
     3601 ctgcgcgaaa ttcaggtcga aagagagcgt ggtctcgaga ccgttcgcaa agccggcttc
     3661 cgccatcagc gccttggcct tgtcgatatc ggtgaagaat ttggtcggct gcggccaggc
     3721 gacttcggtc ggcttgtccg cgggcgcacc gaacatcgga ttggcaagcc cgaacaacac
     3781 cgcgtccatg atcttctggt acgggattgc gtgggcgacc gcctggcgga ctttcaaatt
     3841 gtcgaacggc ggtctggtga cgttcatgcc gatgtactgg atgccgttgg agaacggcag
     3901 cgagatcacg ttgagcttgc tctccgcctt catctcctgg aaatccttgt tcggcagttc
     3961 gtaggagatg tcggcatcgc cgcgttcgag cagcgcgcgg cggttgccgg cctgcggcac
     4021 catgcgccag atcacgcgcc tgatcttcgg cagcggaccg ccgacccatt gatcgttgcg
     4081 ctccatgatc acttcggtgc cggcggtcca tttcgtcacc ttgtaggcgc cggagcccgc
     4141 ggtctgctgc ttggtgtatt cgaggcccca cggatccttc tccgaggcgc tcttcttcac
     4201 cagttccgaa ttgaccacgc agggcacgat gactgcgagg tccggaattg tcaggcggtc
     4261 cttcttggcg aagtcgacgc gaacggtctg gtcatcgacg atgacgaact gttcgggctt
     4321 ggtgagcgag ccggcgctca tctggaacgt cggaaa
//
LOCUS       DATLJT010001040         2747 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2600267,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001040 DATLJT010000000
VERSION     DATLJT010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600267"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VM910_23935"
     CDS             complement(<1..1485)
                     /locus_tag="VM910_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65604.1"
                     /translation="MKGIAEWLASIGLSEYAQRFADNAIDLSVIRDLTEQDLKDLGVL
                     LRHRRKILRAIAELDGVAPAPIETATEPVARHEPERRHLTVMICDLVGSTALSTRLDP
                     EDMRVVIDAYHAACARITRTYDGFLAEFRGDGILAYFGYPIAHEDDAERTVRAGLDII
                     AAVALLETRAGKPLAVRIGIATGLVVVGDLSREGALRERAVVGETPNLAARLQALAEP
                     GTIVIAASTRRLLGDLFRLRDLGLHEVKGIAEPVAAWTVEGVSASESRFEAVRAAGLT
                     DFIGREDELDFLLERQRLAWKGEGQIVLISGEPGIGKSRLVAALAERIAGEPHTRLRY
                     QCSPYHTNSALHPCIAQLERAAGFKADDTPEQRLDKLEAILAMDASRVQAAAPLFAAL
                     LSIPFGERYPLLALSPAQQRRRTLAALLDQFEALARRQPILLSFEDAHWADATSLELL
                     DLMVERVRQLPVLALFTFRPEFEAPWFGLPNVSTLTLGRLNRSNV"
     gene            complement(2166..>2747)
                     /locus_tag="VM910_23940"
     CDS             complement(2166..>2747)
                     /locus_tag="VM910_23940"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65605.1"
                     /translation="VNPSVPAKTVPEFIAYAKANPGKISMASAGIGSGPHVAGELFKM
                     MAAVNTVHVPYRGQGPALTDLLAGQVQFYFAGIPSSIQYVRSAKLRALAVTTTTRSDV
                     LPDIPTLSDFLPGYEASFWGGFCAPKSTPAEVIETLNRAINAALSDPRMQARLADLGA
                     TPFGGSPTDFGKHIAAETEKWAKVIKSAGIKAE"
BASE COUNT          521 a          869 c          870 g          487 t
ORIGIN      
        1 gacgttggag cggttgaggc gaccgagcgt caaggtgctg acattgggga gaccaaacca
       61 gggtgcctcg aactccggtc ggaaggtgaa cagtgcaagg accggcaatt ggcgcacccg
      121 ctcgaccatc agatcgagca gctcgaggga ggtggcatcc gcccagtgcg catcctcgaa
      181 cgaaagcagg atcggttgcc ggcgggcgag agcctcgaat tgatcgagca gtgccgccaa
      241 cgtccggcgg cgctgctgcg cggggctgag ggccagcagc ggatagcgct caccgaacgg
      301 gatcgacagc agcgccgcaa aaagcggcgc ggcggcttga acccgcgatg cgtccatagc
      361 gaggatcgct tccaacttgt cgaggcgttg ctcgggcgta tcgtctgcct tgaaccccgc
      421 cgcgcgctcg agctgagcga tgcacggatg gagtgcgctg ttggtgtggt agggcgagca
      481 ttggtaacgc aagcgcgtgt gcggctcgcc ggcgatgcgc tcggcgagag ccgccacaag
      541 gcgggacttg ccgatccccg gctcgcccga aatcagcacg atctgccctt cccccttcca
      601 ggccagccgt tggcgctcca gcaggaaatc gagctcatcc tcgcggccga tgaaatcggt
      661 cagacccgcc gcgcgcaccg cctcgaagcg actctcggaa gccgatacgc cctccaccgt
      721 ccacgccgcc accggctcgg caataccctt gacctcgtgc aagcccaggt cgcgtagacg
      781 gaacagatcg ccgagcagcc ggcgcgtgga ggcggcgatg acgatcgtcc cgggctcggc
      841 caatgcctga aggcgcgctg caaggttcgg cgtctctccg accacggcgc gctcgcgcaa
      901 tgcaccctcg cggctcaggt cgccgaccac taccaagccg gtcgcgatgc cgatgcgaac
      961 cgcgagcggt tttccggcgc gcgtctcgag tagggcaacg gcggcgatga tatcgagccc
     1021 cgcccgcacg gttcgctcgg catcgtcttc gtgcgcgatt ggatagccga aataagccaa
     1081 gatcccgtcg ccccggaact cggcaaggaa gccgtcatag gttcgcgtga tacgtgcgca
     1141 ggcggcgtga taggcatcga ttacgacgcg catgtcctcc ggatcgagtc gcgtcgacag
     1201 agcagtcgag cccaccagat cgcagatcat cacggtgagg tgacggcgct cgggctcgtg
     1261 acgcgccacc ggttcggtcg ctgtttcaat cggtgcagga gcgaccccat caagctccgc
     1321 gatcgcgcgc agaatcttgc ggcgatgccg aagcagaacg ccgagatctt tgaggtcttg
     1381 ttctgtcaga tcgcgaatga ccgagagatc aatggcgtta tcggcgaacc gttgggcgta
     1441 ctcactcagg ccgatcgatg ctagccactc ggcaatgcct ttcatggcac gtcccgtcga
     1501 tggcgtgccg catcatacgc gcttagtgtc cgggcgccat cgcgcaatgc ttgagtctgg
     1561 agcaggagtt caaccacact gcatcgaagc cgtgcattct cggcttcgag ccgctcaagc
     1621 aatctccgcc tatcgagccc cgtgatttca gaattccgcg gtggcagacg tagtctcttg
     1681 aaggcatgtc cattcgaacc gcctctcagt cgccaagcac gctcacgtgc aagctgcagg
     1741 ctcagtacat gacccatgaa ccatcccccg atccccaccc cacccatccc cccaacccag
     1801 tggttcaatg gtacctatgt cgtccagaat gctcccgatc gccggcgtgt gaacgcaaag
     1861 aagccccgcg ttggcggggc tgagttcggg aggaaaccat cgaacgtgag caccgcatgc
     1921 gcggtaccga cgagtgtgag actagcgccg ccgttgcgcc agcgatgtga tggcgatcac
     1981 aaaaatgcgc ttttttgccg cgtgttgcag agatgtccgc ctgcaggacc gccaagccag
     2041 tcgcgtgtcc gctttctcgg gccggtgcct cttagccgga tcacgtcgtg atgtctcaga
     2101 ggtgccatga gccgtcattt tcgccgaacg ggcgttacgg aatattcggc cgggatcgcg
     2161 tcggatcact ccgccttgat gccggcggac ttgatcactt tcgcccactt ctcggtttcg
     2221 gcagcgatgt gcttgccgaa atcagtgggt gagccaccga acggtgtggc gccgaggtcg
     2281 gcaagccgcg cctgcatcct gggatcggac agggccgcgt tgattgccct gttgagggtt
     2341 tcgataacct ctgccggcgt gctcttaggc gcacagaagc cgccccagaa gcttgcctca
     2401 taacccggca gaaagtcgct caaggtcggg atatcgggca gcacgtccga gcgcgttgtg
     2461 gtggtcaccg ccaaagcgcg cagcttagcg gacctgacgt attggatcga cgaaggtatg
     2521 ccggcgaaat agaactgcac ctgcccggcg agaaggtccg tgagggctgg accctgcccg
     2581 cgataaggca cgtggactgt gttgacagcc gccatcatct tgaacagctc gccggcaaca
     2641 tggggcccgg agccgatgcc agccgatgcc atgctgatct tgcccggatt ggctttagcg
     2701 tacgcgatga actcgggaac cgttttggcc ggaaccgatg gattgac
//
LOCUS       DATLJT010001041         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_989459,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001041 DATLJT010000000
VERSION     DATLJT010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989459"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VM910_23945"
     CDS             <1..659
                     /locus_tag="VM910_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65606.1"
                     /translation="AAYLQRWLEQAPTPGAKNNRLSALKSFFKWAHEVVHLIETNPVE
                     GIKVTATSTPGHHTWTDEEIAQFRARHPTGTRARLALELMIHLAIRRGDGISLGPQHV
                     KNGWVIYTQEKNKRRKPTLVQTPVPEAVRAAIAACPAPETSLTFLVNEWGRPYSKRSF
                     NTAFREWCDEAGLPATCRPQIAQGGLQADGRERLQRARDHGGQRPSHAERGRALHRGV
                     "
     gene            756..1151
                     /locus_tag="VM910_23950"
     CDS             756..1151
                     /locus_tag="VM910_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65607.1"
                     /translation="MSEQSWRRVWRWVVGTRARRSAFTLAVLVAMASTYVTGSYDPDQ
                     STVYRFVPLTAKERAKIIENWGEIILTKNCYFRNDQEPNLLCIIDQGKLKNDGGEESD
                     PYLSLLAYIALNGAIILATLGRVLIIGQD"
     gene            complement(1284..1931)
                     /locus_tag="VM910_23955"
     CDS             complement(1284..1931)
                     /locus_tag="VM910_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HVI65608.1"
                     /translation="MYVPNAFKEERVSILHDAIRRAGLATLVSVTDEGLIASHVPLLL
                     DPEPAPYGTLLGHLARPNPQARRAIGDALAIFLGPDAYITPSWYATKRETGKVVPTWN
                     YVAIHAYGPVEFIDDPVHAREHITHLTNRHEQGRAIPWAPSDAPEDFIANQVRKIIGF
                     RLTIKRLEGKWKMSQNRPAQDRAGTAAGLEREGKRDLAELIRAASPPGEGAGKPR"
     gene            2237..>2867
                     /locus_tag="VM910_23960"
     CDS             2237..>2867
                     /locus_tag="VM910_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVI65609.1"
                     /translation="MRYELTDHEWSAIKPMLPNKPRGVPRVNDRRVLNGIFWVLRSGA
                     PWRDLPDGFGPYTTCYNRFVRWRQAGVWSQIIGALAATHDAAVQMIDTSIVRVHQHAA
                     CITRNRNQSMGRSRGGLTSKIHAMVDTNGLPVRLALTAGEAHDNRLAFKLLSRLKSGT
                     MLLADRGYDADWIRTLVSRRGAWANIPPRRNRNEPICFSPHLYRARNLVE"
BASE COUNT          568 a          869 c          881 g          549 t
ORIGIN      
        1 acgctgcata cctgcagcgt tggctggaac aggctccgac gccgggcgcg aagaacaatc
       61 ggctgtcggc tctgaagtcg ttcttcaagt gggcgcacga ggtcgtgcac ttgatcgaaa
      121 cgaacccggt tgagggcatc aaggtgacgg ccacgtcgac gcccgggcac cacacctgga
      181 ccgatgagga gatcgcgcaa ttccgggccc gtcacccgac cggcacacgc gctcgtcttg
      241 cgctggagct gatgatccat ctcgcgatcc ggcgcggcga cggcatctcg ctgggccccc
      301 agcacgtgaa gaacggctgg gtgatctaca cccaggagaa gaacaagcgg cgcaagccga
      361 cgctggtgca gacgccggtg ccggaggcgg tcagggccgc catcgcggca tgtcctgccc
      421 cggaaacctc gctgacgttc ttggtcaacg aatggggtcg cccgtactcc aagcggagct
      481 tcaacaccgc gttccgtgag tggtgcgacg aggccgggtt gccggcaact tgccggccgc
      541 agattgcgca aggcgggctg caagctgatg gccgagagcg cctgcaacgt gcacgagatc
      601 atggcggtca gcggccatcg cacgctgaaa gaggtcgcgc gctacaccga ggcgtatgac
      661 cgtaagcagg cggcggcgcg cgcgcaggcc aaggtcgcgg ccgcaaagga caacgtcgtc
      721 ccgctggcgc gcgtgcggtg agggaggccg aagccgtgag cgaacagtcc tggcggcggg
      781 tatggcgatg ggtagtaggg acgcgcgcac gccgcagcgc gttcacgcta gcggtgctgg
      841 tggcaatggc gtcgacatac gtcactggca gctacgatcc cgaccaatca acagtttacc
      901 gcttcgtgcc gttgacggca aaagagcgcg ctaagatcat agaaaactgg ggcgaaatca
      961 tactcactaa gaactgctat ttccgaaatg atcaggagcc aaaccttctc tgcatcatag
     1021 atcaaggaaa actcaagaac gacggtggtg aggaatcaga cccgtacctg tcgttgttgg
     1081 catacatcgc tcttaacgga gcgatcatct tggcgacact aggccgtgta ctcataatcg
     1141 gccaggactg atgcaaggca ggcaatgtcc gctcttcctc taacagcggc acaaatgcgg
     1201 tcttcgcttt aggtccgctt tgcccgcctc gactcgttcg gatatgggcg gcaggtggcg
     1261 caagcggacg tccaggcaga ggttcagcgt ggcttacctg cgccctcgcc gggcggcgaa
     1321 gctgctcgga tcagctctgc cagatcgcgt tttccctctc tttccagacc agccgcagtc
     1381 ccggctcggt cttgtgccgg tcgattctga ctcatcttcc atttgccctc gagccgttta
     1441 atcgtcagcc gaaaaccgat gattttcctg acctgatttg cgatgaagtc ctccggcgcg
     1501 tcgctcggcg cccaggggat agctcggccc tgctcatgtc ggttcgtgag atgcgtgata
     1561 tgttcgcgcg catgtactgg atcatcgatg aactcaaccg gtccgtaggc gtgaatggcg
     1621 acgtaattcc aggttggcac tactttgccg gtctctcgct tggtcgcata ccaagatggt
     1681 gtgatatagg catccggtcc gagaaagatc gccagtgcat cgccgatcgc ccgtcgtgcc
     1741 tgcgggttcg ggcgagctag atgacccaga agcgtcccat aaggagcagg ctctggatca
     1801 agcaataatg gcacgtggct ggcgattaac ccttcatcag ttacgctgac cagtgtggcc
     1861 aagccagcgc ggcggattgc atcgtgaagg atactgacgc gttcttcctt gaatgcatta
     1921 ggaacgtaca cgatgttcct ccgattcagc gcggggacag cggaggctat ccgctctcgt
     1981 ccaaatgtgc cagagccgag gggcgcttgg tccgcatcac cggcgcgtca ctggtgtctc
     2041 tcctgggcgg cacggtgcct tgcgagtggg cgcgcgccac agctaaagtc gtggcggctt
     2101 atcaaaaccg gaaagcctca gtcggagcat aagacttcgg cttcgcctcc gaaagccgac
     2161 attcggccga cctagcttgc acgcttggat cggctgccag ttcgtgattc aagcctccaa
     2221 acgggagcct cgaatcatgc gctacgaact cactgaccac gagtggtctg ccatcaaacc
     2281 gatgcttccg aacaagcctc gcggcgtgcc ccgcgtgaat gaccggcgtg tcctcaacgg
     2341 catcttttgg gttttgcggt ccggagcccc gtggcgcgat ctgcccgacg gctttggtcc
     2401 gtacaccacc tgctacaacc gcttcgtccg ttggcgacag gctggggtct ggagccagat
     2461 tattggggcg cttgctgcta cgcatgatgc agccgtgcag atgatcgaca cctctattgt
     2521 ccgcgtgcac cagcacgccg cctgcatcac tcgaaacagg aaccagtcga tggggcgatc
     2581 acgaggtggg ttgaccagca agattcatgc aatggttgat accaacggcc tgccggtccg
     2641 actcgcgctt actgccggtg aggcgcacga caaccggctc gcattcaaac ttctatcccg
     2701 cctgaagtcg ggaacaatgc tgctggccga ccgtggctac gacgcggact ggatcagaac
     2761 acttgtcagc cggcgcggcg cttgggcgaa cattccgcca cgacggaacc ggaacgagcc
     2821 gatttgcttc agcccacatc tctaccgcgc tcgcaacttg gtcgagc
//
LOCUS       DATLJT010001042         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1220660,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001042 DATLJT010000000
VERSION     DATLJT010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220660"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VM910_23965"
     CDS             complement(<1..205)
                     /locus_tag="VM910_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65610.1"
                     /translation="MFNETGCVPGIDGEQVPAERIVPDAKEYISETIDDPETIYDPEI
                     WGTPGFALVLSWLAQKWHRRCHKL"
     gene            complement(350..1321)
                     /locus_tag="VM910_23970"
     CDS             complement(350..1321)
                     /locus_tag="VM910_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65611.1"
                     /translation="MKLPRRNFLHLAAGAAALPAVARLAWAQTYPTRPVRIIVPFPAG
                     LATDTIARLMGQSLSERLGQPFVIENRTGAGGNIGTESVVRATPDGYTLLLVGLSNAM
                     NATLYKKLNFNFIREIAPVASIGGAPYVMVVNPSVPAKTVPEFIAYAKANPGKINMGS
                     TGSGSVSHVFGERFKTMTGINLVHIPYRGGYVSDLLSGQVQIAIGTISTNIQYIRGGM
                     LRALAVTTATRSDVLPDVPTLAEFVPGYETSQWYGVGAPKDTPVEVINTLNKEINAVA
                     ADPLIKARLAGLGVDPLSKTPTEFEKFIADETEKWGKVIRAAGINAD"
     gene            complement(1342..>2182)
                     /locus_tag="VM910_23975"
     CDS             complement(1342..>2182)
                     /locus_tag="VM910_23975"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65612.1"
                     /translation="GLRKGLAEAGFVEGQNLTIEYVWTDGQSERLSALAAELVGRHVL
                     VIVSSALNATYAAKAATSTIPVVFAVNNDPVATGLVASLNRPGGNLTGVAYLTGELGA
                     KRLGLMHEIVPKVADFAVLAHPTYPSSAPFISDVKAAARTLGLRIEVFNASTESEIDA
                     AFAALSARRLGALLLAGHPLFTTRREPIIALAARYAVPTMYTNREFASAGGLITYGPD
                     LTEVYRLTGGYAGRILKGEKAADLPALLPTKFEMIINLRTAKALGLEVPDRLLALADE
                     VIE"
BASE COUNT          407 a          653 c          658 g          464 t
ORIGIN      
        1 cgagcttgtg acatcttcga tgccattttt gtgccagcca cgaaaggacc agcgcaaatc
       61 caggcgtgcc ccatatctcc gggtcgtaga ttgtttcggg atcgtcgatt gtctccgaga
      121 tgtattcctt cgcgtcgggc actatccttt ccgctggcac ttgctctcca tcaataccag
      181 gcacgcaccc ggtttcgttg aacatctcgc tggcttcctc tagaagagcc atacagtctt
      241 tagttattac cctcctcatg atttatctct ccggcgttgg cgaccgtggg tcatgtgacc
      301 gtcgttcgcc gaacgagagg ttatggaata ttggatcggg agccccgcgt cagtccgcat
      361 tgatgcccgc cgcccggatc accttgcccc atttttcggt ttcgtcggcg atgaatttct
      421 cgaattcggt gggtgttttc gacaatgggt caacgcctaa gccggcaagc cgcgccttta
      481 tgaggggatc agccgcaaca gcgttgatct ccttgttgag cgtgttgatc acctcaacgg
      541 gcgtgtcttt cggggcaccg accccatacc attggctcgt ctcgtagccc ggcacgaatt
      601 cggccagggt cggaacatct ggcagcacat ccgaacgggt cgcagttgtc accgccagcg
      661 cgcgcagcat gccgcctctg atgtactgaa tgttcgtgga tatggtgccg atagcgatct
      721 gcacctggcc gctgagcagg tcggaaacgt aaccgccgcg atacggaatg tggaccaagt
      781 tgataccggt catcgtcttg aaccgttcgc cgaagacatg ggacacggac ccgctgccgg
      841 tcgatcccat gttgatcttg cctggattgg ccttggcgta ggcgatgaac tccgggaccg
      901 ttttggctgg aacggacgga ttgaccacca tgacataggg ggcgccacca atactcgcga
      961 ccggcgctat ttcgcggatg aaattgaaat tgagcttctt gtagagtgtt gcattcatcg
     1021 cgttcgatag cccgacaagt aggagcgtat agccgtcagg cgttgcccgc acgacgcttt
     1081 cggtgccgat attgccgccg gcacccgttc ggttctcaat gacgaatggc tgcccgagcc
     1141 gctccgacag cgattgaccc atgagacgcg cgatggtgtc ggttgctagg ccggcgggaa
     1201 aggggacgat gatccgcacc ggccgcgtgg gatacgtctg tgcccaagcg agacgcgcca
     1261 cagccggcag cgcggcagcg cccgctgcga gatggagaaa atttcggcgg ggaagtttca
     1321 tttgaacctc cctccttcac atcattcgat cacctcgtcg gcaagcgcga gcaatctgtc
     1381 ggggacttcg aggccgagcg ccttcgccgt cctaaggttt atgatcatct caaacttggt
     1441 cggcagcagc gccggaaggt cggcagcctt ctcgcccttg aggatgcgac cagcgtagcc
     1501 gccagtcagg cgatacacct cggtcaagtc cggcccatag gtgatgaggc cgccggcgct
     1561 ggcaaattcc cggttcgtat acattgtcgg caccgcgtag cgcgcggcga gtgcgatgat
     1621 cggctcccgg cgagtggtga acagcgggtg gcccgctagc aggagcgcgc ccagcctgcg
     1681 cgcgcttaga gccgcgaaag cggcgtctat ctcgctttca gtcgacgcgt tgaagacctc
     1741 gatccgcaaa cccaaggtgc gggcggccgc cttcacatcg ctgatgaacg gcgcggagga
     1801 cggataggtc ggatgtgcga gcacggcaaa gtcggcaacc tttggaacga tctcgtgcat
     1861 cagccccagt cgttttgctc caagttcgcc ggtcaagtag gccacgccgg tgaggttgcc
     1921 gcccgggcgg ttgaggctcg caaccagccc agttgccacg ggatcgttgt tgaccgcgaa
     1981 tacaactggg atcgtggagg tcgctgcctt ggcagcgtac gtcgcgttga gggcactgct
     2041 cacaatgacg agcacgtgcc ggcccaccaa ttctgccgca agtgccgata accgctcgct
     2101 ctggccgtcg gtccagacat actcgatggt gagattctga ccttcgacaa agcctgcttc
     2161 ggcgagtccc ttgcgcagcc cg
//
LOCUS       DATLJT010001043         2309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1220814,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001043 DATLJT010000000
VERSION     DATLJT010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220814"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1292
                     /locus_tag="VM910_23980"
     CDS             111..1292
                     /locus_tag="VM910_23980"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HVI65613.1"
                     /translation="MSVALASSSTPSLTREQPLRVMVVDDSAVIRGLISRWISAEPDM
                     VVAASLRTGLEAVNQLERINPDVAVLDIEMPELDGISALPRMLAKKRDLVIIMASTLT
                     RRNAEISFRALSLGASDYIPKPESTREAGAADIFHHDLIQKIRHLGARARRTAPGIHG
                     SPLALSSTPARDKSREFVPRPVQARSALSPLVRRAFGTQAPRVLLIGSSTGGPQALMA
                     LTTELGPVIDRYPVLITQHMPPTFTTILAEHLARASRRPAHEAIDGEAVKPGQIYVAP
                     GGRHMRVIRHGVDAAIALDDGPPVNYCKPAVDPLFTSAIEVWRGGILALVLTGMGSDG
                     MRGGKEIVAAGGSVIAQDEATSVVWGMPGAAANAGICAAVLPLNQIAPKVTRLFAGDR
                     S"
     gene            1289..2161
                     /locus_tag="VM910_23985"
     CDS             1289..2161
                     /locus_tag="VM910_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HVI65614.1"
                     /translation="MTPLDYEYLRKLLKERSGLDLSSDKQYLVESRLVPLARKAGLGG
                     IPELVQKMRSGAEALATEVVEAMTTNETFFFRDKIPFDHLRDTVLPALLPARAGRRSL
                     RIWSAASSTGQEPYSIAMLLKEKAQMLGGYRIEIVATDLSQEVLEKSKAGIYSQFEVQ
                     RGLPIQLLVKYFTQTGELWQLNADVRSMVQHRQLNLLQDFSHLGKFDVIFCRNVLIYF
                     DQDTKINIFERLNRVLEPDGVLMLGAAESVVGITDAFRACPDRRGLYQPNALRSPRPA
                     AGAIVPGLKVVATR"
     gene            complement(2177..>2309)
                     /locus_tag="VM910_23990"
     CDS             complement(2177..>2309)
                     /locus_tag="VM910_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HVI65615.1"
                     /translation="SFSQQFAILGSWLVGHTPCGLSIREDSSPITGNNSRFLPHAIL"
BASE COUNT          394 a          746 c          740 g          429 t
ORIGIN      
        1 tccaggaagt tggcctgatc tgaccttcag ttggtgttgt cggcgatccg gtggctcggc
       61 ggccctcgcc gtagtgtctg tgtgtgccgc ttgagttgct ctggtgaccg atgagtgttg
      121 cgttggcaag ttcctcgacc ccgagtttga cgcgcgagca gccgttgcgg gtgatggtgg
      181 tcgatgattc cgcggtcatc cggggactga tttcccgctg gatttccgcc gagccggaca
      241 tggtggtcgc ggcttcgctg cgtaccgggc tcgaggcggt caaccagctt gaacgcatca
      301 accccgacgt cgcggtgctc gacatcgaga tgccggaact cgacggcatc tccgctttgc
      361 cgaggatgct cgccaagaag cgcgatctcg tcatcatcat ggcctccacg ctgacccgcc
      421 gcaacgcgga gatcagcttc agggcactgt cgcttggcgc atccgactac attccgaagc
      481 cggagagcac gcgcgaggca ggcgccgccg atattttcca ccacgacctg atccagaaaa
      541 ttcgccatct cggcgcgagg gcgcgtcgca ccgcgccggg gatccacggt tcgccacttg
      601 cgctctcgtc cacgcccgcc cgtgacaaat cgcgcgaatt tgtcccgcgc ccggttcagg
      661 ctcgctcggc gctgtcgcct ctggtgcgcc gcgccttcgg cacgcaggcg ccgcgcgtac
      721 tcctgatcgg ctcgtcgacc ggcggaccgc aagccttgat ggcgctgaca accgaactcg
      781 gcccggtgat cgaccgctat ccggtgctga tcacccagca tatgccgccg accttcacca
      841 cgatcctcgc cgagcatctg gcgcgcgcga gccggcggcc ggcgcacgag gccatcgacg
      901 gcgaggccgt caagcccgga cagatctatg tcgccccagg cggccgccac atgcgcgtca
      961 tacgtcatgg agttgacgcg gcgatcgcgc tcgatgacgg cccgcccgtc aattattgca
     1021 agcccgcggt cgatccgctg ttcacctccg cgatcgaggt ctggcgaggc ggcattctcg
     1081 cgctggtgct gaccggcatg ggctcggacg gcatgcgcgg cggcaaggaa atcgttgcgg
     1141 cgggcggcag cgtgatcgcc caggacgaag cgaccagcgt ggtgtggggc atgcccggcg
     1201 cggccgccaa tgccggcatc tgcgccgcag ttctgccgct caatcagatc gcaccgaaag
     1261 tgacgcggtt gtttgcagga gatcgttcgt gacgcccctg gattatgagt atctgcgtaa
     1321 actcctgaag gagcgctcgg gtctcgacct gtcttccgac aagcaatatc tggtcgaaag
     1381 ccggctggtg ccgctggcgc gcaaggccgg gctcggcggc atccccgaac tcgtccagaa
     1441 gatgaggagc ggcgccgagg cgctggcgac ggaagtggtc gaggccatga ccaccaacga
     1501 gacgttcttc ttccgcgaca agatcccgtt cgatcatttg cgcgacaccg tcctgccggc
     1561 gctattgccg gcgcgcgcgg gccgtcgcag cttgcggatc tggtctgccg cctcctcaac
     1621 cgggcaggag ccgtattcga tcgcgatgct cctgaaagag aaagcccaga tgcttggcgg
     1681 gtatcggatc gaaatcgtcg ccaccgacct gtcgcaggaa gtgctcgaga agtcgaaggc
     1741 cggcatctac agccagttcg aagtgcagcg cggactgccg atccagttgc tggtgaaata
     1801 tttcacgcaa acgggcgagc tttggcagct caatgccgac gtccgcagca tggtgcagca
     1861 ccggcaattg aacctgctgc aggacttctc ccatctcggc aaattcgacg tcatcttctg
     1921 ccgtaacgtg ctgatctatt tcgatcagga caccaagatc aatatcttcg agcggctcaa
     1981 cagggtgctc gagcccgacg gcgtgctgat gctcggtgcc gccgaatccg tggtcggcat
     2041 caccgacgct ttccgcgctt gtcccgaccg gcgcgggctc tatcagccca acgccttgcg
     2101 gagcccgcgc ccggccgccg gcgcgatcgt gccggggctg aaagtggtcg cgacgcgctg
     2161 aaacgtatcg tgcggttcac aggatcgcat gcggcaggaa gcgcgaattg ttgccggtga
     2221 tcgggcttga gtcctcgcgg atcgacagtc cgcaaggggt gtggccgacc agccagcttc
     2281 cgaggattgc aaattgttgc gaaaaactc
//
LOCUS       DATLJT010001044         1690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3289053,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001044 DATLJT010000000
VERSION     DATLJT010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289053"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /locus_tag="VM910_23995"
     CDS             complement(<1..>1690)
                     /locus_tag="VM910_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVI65616.1"
                     /translation="PCHRAFALPLKFASASYIGSLDPDVLWEMLIGGIVVCAFFATLA
                     LWIHSALRRVKRSQARRHAYISSALNSLSHGVAMTDPKMRVVYCNDRYLEIYGMTRSD
                     LTPGMMGSELLELRLKRGLLDVSVEDFYRLASRPEGLITELPNGRSVLAKYFPLPSGG
                     SIATHEDCSEQRKLSRQLASTKQFLESMLDNVPVCVAAKSIEDGRYIFANRAFERFSR
                     FSRDHIVGKRADEIFRPATAAAIQDADRAAVESPNGHYRNEFAVERGKEKRLLASNRV
                     IARNDRNEPEFLIAMFDDVTDRRSLSRELENAKQFLELVVDNIPVSLIVERVSDGRYL
                     LANRSAEAILGRRREDVTGLTAADIFNPREAKLIIARDEAAIRKRALLTEEHPISTKD
                     GLRLFLTRRMTVLNESGEPQYLIKTHEDVTDRRQTESRMAHMAYHDGLTDLPNRAAFL
                     KALAQMIEACAGSSEEFAVLCVDLDGLKEVNDVFGHATGDKLLVEVARRIQAGARGGV
                     VARLSGDEFGLIIDGKQPFSGIALAEKLFEAMSNEFQIDGKSVRTGITTGISVFP"
BASE COUNT          301 a          527 c          543 g          319 t
ORIGIN      
        1 cgggaatacc gagatgccgg tcgtgatccc ggtgcgaacc gacttgccgt cgatctgaaa
       61 ttcattggac atcgcttcga aaagcttctc ggccagcgca atgccggaga agggttgctt
      121 gccgtcgatg atcagaccga actcgtcgcc ggacagccgc gccacgacgc cgccacgcgc
      181 gcctgcctgg atccggcgcg ccacctcgac caggagcttg tcgccggtcg cgtgtccgaa
      241 cacgtcgttg acttccttca ggccgtcgag gtcgacgcac agcacggcaa attcctcgct
      301 gctgccggcg caggcctcga tcatctgcgc cagcgccttc aggaacgcgg cacggttcgg
      361 cagatccgtc aggccgtcgt gataggccat gtgcgccatg cgggattcgg tctgccggcg
      421 gtcggtgacg tcctcatgcg tcttgatcag atattgcggc tcgccgcttt cgttcagcac
      481 cgtcatgcgg cgggtcaaaa acagccgcag gccgtccttg gtcgagatcg gatgctcttc
      541 ggtgagcaaa gcgcgtttcc tgatcgcagc ctcgtcgcgc gcgatgatca gctttgcctc
      601 gcgcggattg aagatatccg cggccgtcag ccccgtgacg tcttcgcggc ggcgcccgag
      661 gatcgcttcg gcgctgcggt tggcgagcag atagcgcccg tcgctgacgc gctcgacgat
      721 caacgacacc ggaatgttgt cgacgacgag ctcgaggaac tgcttggcgt tttcaagttc
      781 acgcgacagc gatcgccgat cggtgacgtc gtcgaacatc gcgatcagga attcgggctc
      841 gttcctgtcg ttgcgcgcga tcacgcgatt ggaggcgaga aggcgcttct ccttgccgcg
      901 ttcgacggca aactcgttgc ggtaatgccc gttcggcgat tcgaccgccg cccgatcggc
      961 gtcctggatg gcggccgccg tggccggccg gaaaatctcg tcggcgcgct tgcccacgat
     1021 gtggtcgcgc gaaaagcgcg agaagcgttc gaaggctcga ttggcgaaaa tgtagcggcc
     1081 gtcctcgatg ctcttggcgg ccacgcagac cggcacgttg tcgagcatcg attccaggaa
     1141 ctgcttggtc gacgccagtt gtcgcgacag cttgcgttgc tcgctgcaat cctcatgggt
     1201 tgcgatcgaa ccgccgctgg gcagcgggaa gtacttcgcc aatacggacc gcccgttcgg
     1261 cagttcggta atcagaccct cgggccgtga cgcgaggcga tagaaatcct ccacgctgac
     1321 gtcgagcagt ccgcgcttga gccggagttc cagaagctcg gaccccatca tgcccggtgt
     1381 gaggtccgaa cgcgtcatgc cgtagatttc cagataacga tcgttgcaat agacgacccg
     1441 cattttgggg tcggtcatcg cgacgccatg gctgaggctg ttcagcgctg agctgatata
     1501 ggcgtgccgg cgcgcctgcg agcgcttgac ccggcgcagc gccgaatgga tccagagcgc
     1561 gagcgtcgcg aaaaacgcac agacgacaat cccgccgatc agcatctccc agagcacgtc
     1621 gggatcgagc gaaccgatat agcttgcgga cgcgaatttg agcgggagtg cgaaggctcg
     1681 atggcatggc
//
LOCUS       DATLJT010001045         6351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2369054,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001045 DATLJT010000000
VERSION     DATLJT010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369054"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1767
                     /locus_tag="VM910_24000"
     CDS             160..1767
                     /locus_tag="VM910_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVI65617.1"
                     /translation="MSGIVLSAAVRQNLLSLQSTASLLATTQNDLATGLKVNSALDNP
                     TNYFTAQGLNNRASDISNLLDAIGNGVQVLQAANTGITSLQSLVDSAKSIANQVLQSP
                     VGYSTKSNVISTAIAGANANNLLGTSQNSTVTGSTIKNDNSSSVDITTATQLVGTAGS
                     SSNDLATAITSGSTLTIDGKTITFSTGQTTITSDGNNDYTIGIGPGSTLTVGNVLSTI
                     DAITGASTASTVNGSGQIVLSTGTTQNLVISGTGNALSELGIAATTTNLTPPALSGQT
                     LTIGATGGGIATNITFGTATGEISTLNQLNTALAANNLQATIDTTGRINITTSNDAAS
                     YTIGAIGGSAAIGSTTPFYNLMAAAPVVDPNSQSQRASLVAQYNNVLAQINTTAQDAS
                     FNGVNLLNGDTLKLVFNETGKSTLNITGVVFNTTGLGLTTLAAGTDFLDNSSANKAIA
                     SLNSASTTLRSDASSLGSNLSIVQIRQDFNKSLINVLQTGSSNLTLADTNEEAANSQA
                     LSTRQSIAVSALALANQSQASVLQLLR"
     gene            2079..2714
                     /locus_tag="VM910_24005"
     CDS             2079..2714
                     /locus_tag="VM910_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI65618.1"
                     /translation="MADKPASRGEIFVVDDDPAVRETLSVVLSVSGYEVVCFADGAAF
                     LEVARNRTPAALLLDVHIPGKSGLDILRELHGEDYPAPIFMISGQGDIAMAVSAIKNG
                     ALDFIEKPFRGTDLVTRLDEAIDAYARRQEKTSPSRIATLQFPGREPLTRREREVLEQ
                     FTGGASNKEAGRTLGISPRTIEDHRANIMKKLGARNAADLVRIVMTAQRAS"
     gene            2838..4583
                     /gene="araD"
                     /locus_tag="VM910_24010"
     CDS             2838..4583
                     /gene="araD"
                     /locus_tag="VM910_24010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HVI65619.1"
                     /translation="MTDKPRKKAAEALRSARWFAPDDLRAFGHRSRAMQMGYAPQEWK
                     DRPVIAILNTWSDAQPCHMHFKSRVDDVKRGILMAGGFPIELPALSLSESLLKPTTML
                     YRNLLAMDAEELLRGHPVDGVVLMGGCDKTTPALLLGATSMNLPAIYLPAGPMLPGNW
                     KGKTLGSGSDAWKYWDERRAGKISDKDWLDMEAGIARSYGTCMTMGTASTMTAIAEAI
                     GMTLPGASSIPAADANHIRMASECGRRIVEMVWEDLTPKQIQTREAFENAIAVAMAMG
                     CSTNAIIHLIAQARRAGQDIGLDDFEKASRKVPVIANVRPSGEAYLMEDFFYAGGLPG
                     LMSRIRDHLHLDCLTVTGKTLGENIAQASVYDDDVIRPIDNPIYKEGALAVLKGNLAP
                     DGCVIKPSACEARFLRHAGPALVFDDYPSMKKAIDDPDLDVTADHVLILRNAGPQGGP
                     GMPEWGMLPIPTKLVRQGVRDMVRLSDARMSGTSYGACILHVAPESFVGGPLALVKNG
                     DRITLDVAARTINLDVPEAELARRRAAWKPPARRFERGYGWMFSRHIRQANEGCDFDF
                     LETGFGAPVGEPAIY"
     gene            4639..5358
                     /locus_tag="VM910_24015"
     CDS             4639..5358
                     /locus_tag="VM910_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HVI65620.1"
                     /translation="MTRLSDTTRNKLKTVSTATVATALFKRGFRIQCIQDVHPLSANQ
                     PTMVGEAFTLRYMPAREDLNKLDVFRDRAHPQRKAVEECPPGAVLVMDSRKDARAASA
                     GAILVTRLMKRGVAGVVTDGGFRDSGEIAKLGFPSYHHRPSAPTNLTLHQAIEVNVPI
                     GCGDAPVFPGDVILGDADGVIVIPAHLADEIADETFEMTAFEDFVAEEVGKGRGIFGL
                     YPATDEQTLKDFAAWRKNVGR"
     gene            complement(5528..6088)
                     /locus_tag="VM910_24020"
     CDS             complement(5528..6088)
                     /locus_tag="VM910_24020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HVI65621.1"
                     /translation="MPTSIKKDFPTYNVRRFLEPGPIVLVSSAHKRQTNIMTMGWHMM
                     MEFSPALVGCLISGANHSFELIRKSRQCVINLPTTDLAATVVRIGNCSGRDIDKFSEF
                     GLTPEVGTHVRAPLIGECYANFECRLADSGWVKKYNVFVFEVVKAHVATSPKLPKTIH
                     YRGDGEFMLSGEETRRWRKLFRPGML"
     gene            complement(6121..>6351)
                     /locus_tag="VM910_24025"
     CDS             complement(6121..>6351)
                     /locus_tag="VM910_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVI65622.1"
                     /translation="ILAMGGLTDPRVTYWEPAKWIARLRATMGGGGPVLLRTNMGAGH
                     GGASGRFNRLDEVAIVYAFALWAVGLAEKGEV"
BASE COUNT         1213 a         2070 c         1955 g         1113 t
ORIGIN      
        1 ttaacgggtc attagtcata atgacgcggc gacggtgttt tattcggctc ggcgtctccg
       61 gcattaacca acggataagg cttccgggaa caatgtggca aatcgcccgc agaaccgtgc
      121 ggcgatgcgt cagtcgtgtt catccagaag ggaaccagta tgtccggtat cgttctttcg
      181 gccgcggttc gccagaacct gctctcgctg cagtcgaccg cttcgctgct tgccaccacc
      241 cagaatgacc tcgccaccgg cttgaaggtc aactcggctc tcgacaaccc gaccaactac
      301 ttcaccgcgc agggcctcaa caaccgcgcc agcgacatct cgaacctgct cgatgcgatc
      361 ggcaacggcg ttcaggtgct gcaggccgcc aacaccggca tcacttcgct gcagagcctg
      421 gtcgacagcg ccaagtcgat cgccaaccag gtgctgcaga gcccggtcgg ttattcgacc
      481 aagtccaacg tgatctcgac cgccatcgcc ggcgcgaatg ccaacaacct gctcggcacc
      541 tcgcagaaca gtaccgtcac cggcagcact atcaaaaacg acaacagcag ctcagtggat
      601 atcaccaccg cgacccaact ggtcggcacg gcgggcagta gctccaacga cctcgccacc
      661 gccatcacga gcggctcgac actgacgatc gacggcaaga ccatcacctt ctcgacgggg
      721 cagacgacca tcacgagcga tggaaataat gactacacca tcggcatcgg cccgggttcg
      781 acgcttacgg tcggcaatgt tctgtctacg atcgacgcaa tcaccggggc ctcgactgcc
      841 tccacggtca acggctccgg ccagatcgtg ctctcgactg gcaccaccca gaatctggtg
      901 atttcgggta caggcaacgc gctctcagag ctcggcatcg ccgccaccac taccaacctc
      961 acgccgccgg cactttcggg ccagacgctg accatcggcg cgaccggcgg cggcatcgcg
     1021 accaacatca ccttcggcac cgctaccggc gagatctcca cgttgaatca gctcaacacg
     1081 gcgctggcgg cgaacaacct gcaggccacg atcgatacga ccggcaggat caacatcacg
     1141 acctcgaacg atgcggcgtc ctatacgatc ggcgccatcg gcggttcagc agccatcggt
     1201 tcaacgacgc cgttctacaa tctgatggct gcggccccgg ttgtggaccc gaactcgcag
     1261 tcgcagcgtg cgagcctggt ggcgcagtac aacaacgtgc tggcgcagat caacaccacc
     1321 gcgcaggatg cctccttcaa cggcgtcaac ctgctcaacg gcgatacgct gaagctggtg
     1381 ttcaacgaaa ccggcaaatc cacgctgaac atcaccggtg tggtgttcaa cacgaccggc
     1441 ctcggcctca ccacgctggc cgccggcacc gacttcctgg acaactcgtc ggcgaacaag
     1501 gccatcgcct ccctgaactc ggcctccacc acgctgcgtt cggacgcttc ctcactgggt
     1561 tcgaacctct cgatcgtgca gatccgtcag gacttcaaca agagcctgat caacgttctg
     1621 cagaccggtt cgtcgaacct gaccttggcc gacaccaacg aggaagccgc caacagccag
     1681 gcgctgtcga cccgccagtc gatcgcggtc tcggcactgg cgctcgccaa ccagtcgcag
     1741 gccagcgtgc tgcagctcct gcgctaagtc gcgggctgcc aaaaaagatc gaagcggcgg
     1801 ggcaaagccc cgccgtttct gtttcagcaa tcggatagcg gtccgtccgc cgcgcgtgtc
     1861 ttcgcgtcgg gcggcgtttt tttccgcggc cgccatgccg ccgcgctgcg atcgacccct
     1921 gcaccgcgga accgaggttc acattgtcgg ccgtcagtgt ccggcgatac ttgtgagttg
     1981 agttctggtc ccgtacaaat acgggtacgc tcgaatgcgt ggaaccgagc accgagtcgt
     2041 tctccgaacg cgtgtcatgt ctcacaggac gatggcgcat ggctgataaa cctgcttccc
     2101 gcggcgaaat cttcgtggtc gacgacgatc ccgccgtccg cgagactctc tccgtggtcc
     2161 tgtcggtcag cggatatgag gtggtctgct ttgcggacgg cgccgcgttc ctggaggtgg
     2221 caaggaaccg gacccccgcg gcgcttctgc tcgacgttca cattcccggg aaatcgggcc
     2281 tcgacattct cagggaattg cacggcgagg attatcccgc cccgatcttc atgatctcgg
     2341 gacagggcga tatcgccatg gcggtcagcg cgatcaagaa cggggcgctc gacttcatcg
     2401 agaagccgtt ccgcggcacc gatctcgtga cccggctcga cgaggcgatc gacgcctatg
     2461 cacggcggca agagaaaacg tcgccttcgc gcattgcgac gctgcaattc ccgggacggg
     2521 aaccgctgac gcggcgggag cgcgaggtgc tcgagcagtt cacgggcggc gcctcgaaca
     2581 aggaggccgg acggacgctt ggcatcagcc cgcggaccat cgaggatcac cgcgccaaca
     2641 tcatgaagaa actgggggcg cgaaacgcgg ccgatctggt gcggatcgtc atgacggcgc
     2701 agcgcgcgtc ctagtaaaga gtcaatctcg ccaggtccga tttttgcgct atcgtcaacg
     2761 ccttgacgga ggcgggcggg gctcgcccgg gctcggctgc ccgtgagaag agctgacgag
     2821 gtcaacgatg gtgcagcatg actgacaaac cacgcaagaa agctgcggag gcgctgcgca
     2881 gcgcgcgctg gttcgcacct gacgatctgc gcgcctttgg ccatcgctca cgtgctatgc
     2941 agatgggtta cgcgccgcag gagtggaaag accgcccggt gattgcgatc ctcaacacct
     3001 ggtcggacgc ccagccctgc cacatgcatt tcaagagtcg cgtcgatgac gtcaagcgcg
     3061 gcatcctgat ggccggcggc ttcccgatcg aattgccggc gctgtcgctg tcggagtcgc
     3121 tgctcaagcc gaccaccatg ctctaccgca atctgctcgc gatggatgcg gaggaattgt
     3181 tgcgcggcca tcccgtggac ggcgtcgtgc tgatgggcgg ttgcgacaag accacgccgg
     3241 cgctgctgtt gggggcgacc agcatgaacc tgcccgcgat ctatctgccg gcggggccga
     3301 tgctgcccgg caattggaag ggcaaaacgt tgggctcggg ctccgacgcc tggaaatatt
     3361 gggacgaacg gcgggccggc aaaatctccg acaaggactg gctcgacatg gaagccggga
     3421 tcgcccgcag ctacggcacc tgcatgacca tggggacggc gtccaccatg acggcgatcg
     3481 cggaggcgat cggcatgacg ttgccgggcg cctcctcgat tcccgcggcc gacgccaacc
     3541 acatccgcat ggcatccgaa tgcggccgcc gcattgtcga gatggtgtgg gaggacttga
     3601 cgccgaagca gatccagacc cgcgaggcct tcgaaaacgc cattgcggtg gcaatggcga
     3661 tgggctgctc gaccaacgcg atcatccatc tgatcgcgca ggcgcgccgt gccggtcagg
     3721 atatcgggct cgacgatttc gagaaggcga gccgcaaggt gccggtgatc gccaacgtgc
     3781 ggccctcggg cgaggcatat ctgatggagg attttttcta cgccggcggc ctgccggggc
     3841 tgatgagccg catcagggat catctgcatc tcgattgcct caccgtcaca ggcaagaccc
     3901 tcggcgagaa catcgcgcaa gcctcagtct atgacgacga cgtcatccgg ccgatcgaca
     3961 atccgatcta caaagagggc gcgctcgcgg tgctgaaggg caatctcgcg cccgacggct
     4021 gcgtcatcaa accgtccgcc tgcgaggcgc gtttcctcag gcatgccggg cccgcgctgg
     4081 tattcgacga ttatccgtcg atgaagaagg cgatcgacga tcccgatctc gatgtgaccg
     4141 ccgaccacgt gctgatcctg cgcaatgcgg ggccgcaggg cgggccgggc atgcccgaat
     4201 gggggatgct gccgatcccg acgaaactgg tgagacaggg cgtgcgcgac atggtgcggc
     4261 tgtcggatgc gcggatgagc ggcaccagct acggcgcctg catcctgcac gtcgcgccgg
     4321 aatcctttgt cggcggaccg ctggcattgg tgaagaacgg cgaccgcatc acgctcgatg
     4381 tcgccgcgcg gaccatcaat ctcgatgtgc cggaggcgga attggccagg cgccgcgctg
     4441 catggaagcc gcccgcgcgc cgcttcgagc gcggttatgg ctggatgttc tcgcgtcata
     4501 tccgccaggc caatgaaggc tgcgatttcg attttctcga gaccgggttc ggtgcgccgg
     4561 tcggcgagcc ggcgatttac tgaccgtcgt ttccggagtt cgtgcttcgc gctctggaac
     4621 gacagcaagg attgacccat gaccagactc agcgacacca cccgcaacaa attgaaaacc
     4681 gtctcgaccg ccaccgtcgc gaccgcgctg ttcaagcgcg gttttcgcat ccagtgcatt
     4741 caggacgtcc atcccttgag cgccaatcag ccgaccatgg tcggtgaggc cttcacgctg
     4801 cgctacatgc cggcgcgcga ggacctcaac aagctcgatg tctttcgcga ccgcgcccat
     4861 ccccagcgca aggcagtgga ggagtgcccg cccggcgcag tgcttgtcat ggacagccgc
     4921 aaggatgcgc gtgcggcgtc cgccggcgcg atccttgtga cgcggctgat gaagcggggc
     4981 gtggccggtg tcgtcaccga cggcggcttt cgcgactcgg gcgagatcgc aaagctcggc
     5041 tttccctcct accaccaccg gccgagcgcg ccgaccaatt tgacgctgca tcaggcgatc
     5101 gaggtcaatg tgccgatcgg ctgcggcgat gcgccggtgt ttcccggcga cgtcatcctg
     5161 ggagatgccg atggggtcat cgtcattccc gcgcatctcg ccgacgaaat cgcggacgag
     5221 actttcgaga tgacggcatt tgaggatttt gtcgcggaag aggtcggcaa gggcaggggc
     5281 atcttcggcc tctatccggc aacggacgag cagacgctga aggattttgc agcgtggcgg
     5341 aagaatgtcg ggcggtagct cgcgccaaaa cacgatgtcg tcccgggcaa gcgaagcgac
     5401 ccgggaccca taaccccggg ccttcgttgc tgcgacggcc gtcgagccgc taaagatgga
     5461 ttccgcggta tgggtcccgg ctttcgccgg gacgacgatc ttcgtgcctt cactctttcg
     5521 catgcgctca cagcattccc ggccgaaaca gcttccgcca tctccgcgtc tcttcgccgg
     5581 agagcatgaa ctcgccgtcg ccgcgataat gaatcgtctt tggaagtttg ggcgaggtcg
     5641 cgacatgcgc cttcaccacc tcgaagacga acacattgta tttcttgacc cagccggagt
     5701 ccgcaagccg gcactcgaaa ttggcatagc attccccgat cagtggggcg cgcacatgtg
     5761 tgccgacttc gggcgtgagg ccaaattcgg aaaacttgtc gatgtcgcgc ccggagcagt
     5821 tgccgattct gaccacggtc gctgcaaggt ccgtcgtcgg cagattgatc acgcattgcc
     5881 ggcttttgcg gatcagttcg aagctgtggt tggcgcccga gatcaggcag ccgaccaggg
     5941 ccggcgaaaa ttccatcatc atgtgccagc ccatggtcat gatgttggtc tggcgcttgt
     6001 gggccgagct taccagcacg atcgggccgg gctcgagaaa gcggcgcacg ttgtaggtgg
     6061 ggaaatcctt cttgatggag gtcggcatgt ccttctccgc gtcggggaca cgggatcgaa
     6121 ctacacctcg cccttctccg ccagtcccac cgcccacagc gcaaacgcat agacgatcgc
     6181 gacctcgtcg agccggttga agcgcccgga cgcgccgcca tggcccgcgc ccatattggt
     6241 gcgcaacagc accggaccgc cgccgcccat cgtggcgcgc agccgcgcga tccacttcgc
     6301 cggctcccaa taggtgacgc gtggatcggt caagccgccc atcgcgagga t
//
LOCUS       DATLJT010001046        10329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4207603,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001046 DATLJT010000000
VERSION     DATLJT010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207603"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VM910_24030"
     CDS             <1..223
                     /locus_tag="VM910_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI65623.1"
                     /translation="HAWCQVYLPGAGWVEFDPTNGIIGNRDLIRVAVARDPSQAVPLS
                     GTYVGNSEDELGMKVEVSVNCESGPRNRQ"
     gene            complement(693..2399)
                     /locus_tag="VM910_24035"
     CDS             complement(693..2399)
                     /locus_tag="VM910_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVI65624.1"
                     /translation="MVERLSGVKASVGFASETGPRQRNEDFAGAVFGLELPEPRRDVV
                     AAIADGIGGAKGGRVAAETAVRGFLDGFCDLPETMEVRRAAARVLNALNGWIYSQGQR
                     DAKLAGMGCTFTALVLRGRVAHVLHVGDTRAYRLGGDRLTSLTIDHVREGGTGRSSIL
                     NRALGVETEVRLDYAAQPMARHDRFVLCSDGVHGILTAETIADILRERSASEDTARAL
                     VAAALRSGSTDNCTALVLDVVEVPTAESADIGAAIMQLPLIPVPVAGETIDGFVLKVL
                     VSDGRYARLFGAVDEVEGGEVVLKFPKPQVASVATYRAAFVREAWVGARVHSPWVGRT
                     IELPPGRQTCLYTVMPLYPGELLATRLARSPSVGLEEGRNIAIKLARGAAALHRAGII
                     HRDIKPDNVILEGDESLKLIDLGVVRVPGLEDFPPEDIPGTLGYMAPEMLAGEPGNEA
                     TDIYALGVTMFRAFTSEFPYGNRDATSPPRRNRPTALSALRPDLPAWLQAALGRAIAI
                     DPTERFRDMLEFAVEMETGPARAPLAVRKPRTLYERYPLLFWQGLAALLALALLFSLL
                     RH"
     gene            complement(2486..2776)
                     /locus_tag="VM910_24040"
     CDS             complement(2486..2776)
                     /locus_tag="VM910_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65625.1"
                     /translation="MTIGMAFPAINGEAQRGETAITCTNPVSGASWQIRIDYDRSTVD
                     SNPARVTDSKITWHDATNGGNYTLDRKSGNLTVIVASSTGGYFLYDRCRLEN"
     gene            2996..4318
                     /locus_tag="VM910_24045"
     CDS             2996..4318
                     /locus_tag="VM910_24045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVI65626.1"
                     /translation="MALVPSPQWLDTGNNAWQLAAATFVGLQSVPGLTILYGGIVKKK
                     WAINSAFMAMYAFASVLVVWVLFAYNMAFGPQWFPFLGTPGLATSAMFTTGQATVPAA
                     ASGMPPLTFPMATLIFFQFVFAAITVIILGGSLLGRMNFTAWMIFCPVWTTLVYTVGA
                     FSLWGGGWLAGMGVADFSGGYVIHLAAGTSGFVAAAMVGPRLQQDRDHFPPNSLLVTL
                     AGAGILWLGWNGFNGGDPYFANADAGAAVLNTNTATATALLVWTLMDKMAYGKPSVIG
                     AVNGMIAGLVAITPAAGYVDGWGAIIIGVAAGIIPWASMNKLQKTKLMMKADDTLSVL
                     STHGVAGLTGGLLVGILGNPDMLQYIGTEKEAPGVAVTGWLYGNAPQLLLQAEGAAFI
                     IVYNVIATFIILKIISFIVPLRMDDATLKVGDDAVHGETAYAIGVEGE"
     gene            4674..4856
                     /locus_tag="VM910_24050"
     CDS             4674..4856
                     /locus_tag="VM910_24050"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65627.1"
                     /translation="MPYLAMSRLYIDRILRGESPADLPMQAPTKFELVINLRTARTLG
                     LDVPPSLLARADDVIE"
     gene            4964..6229
                     /locus_tag="VM910_24055"
     CDS             4964..6229
                     /locus_tag="VM910_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HVI65628.1"
                     /translation="MRSKLTTIILIGMVLGILVGYACHILWPDPKTAKTIADYISLFT
                     DIFLRLIKMIIAPLVFSTLVVGVAHMGDTKAVGRIGAKAMVWFIGASLVSLLVGMILV
                     NVLRPGDNLNLPLPDAEASTNLKVASLSMKEFVAHLVPRSVVEAMATNEILQIVVFSL
                     FFGVACAALGERTRKLVEGIEQLSYVILSITGYVMALAPIAVFASMAAIVTTQGLGIL
                     VTYGKFVIEFYFGLAVLWCLLIAVGFLIFGHHSFRLVNLIREPFLLAFSTASSEAAYP
                     KTMEQLEKFPISKKIISFVLPMGYSFNLDGSMMYCTFATLFIAQAYNIELPLGQQITM
                     LLLLMLTSKGMAGVPRASLVVIAATLASFNIPESGLLLIIGIDQFLDMGRSATNVIGN
                     TLATAVIAKWEGERVDLGELEAALAPKAA"
     gene            6229..7191
                     /locus_tag="VM910_24060"
     CDS             6229..7191
                     /locus_tag="VM910_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI65629.1"
                     /translation="MRVMRASCASRRTADAGNGLGWPRIVITAAFVLILAAPANPQEA
                     ANVGALSGTLKKVKDTVTITLGYRESSLPFSYLNRRQQPIGYSIDLCREIVEDVSTEL
                     DGMELKIAFAPVTPANRLQKVASGEIDLECGSTTGNVQRRKEVAFSPIFFVAGTKLMV
                     PRSSSVRSYRDLAGKTAVVTAGTTNEAALRTLSDKQKLSITIVTAPDHAQSLATLVSG
                     KADAFATDDVLLYGFIATAKDARDMKVVGEYLSYDPYGLVYRRDDPAFAAVVERAFTR
                     MASERRLAELYNKWFLRRLPTGETLNLPMSPQLEEVFRMLGQPE"
     gene            complement(7413..8903)
                     /locus_tag="VM910_24065"
     CDS             complement(7413..8903)
                     /locus_tag="VM910_24065"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HVI65630.1"
                     /translation="MKFMDVRQWIGLLEKEGELRRITAEVDWHREIGAIARRVLEKKG
                     PALLFEGIKGYRSGRCTKLFVSGLGAQSRLALALGFPRDVANRELIQHVMKKNRETVA
                     PIIVETGPVKDIIVRGEAIDQTEFPVPQWQYLEGGRYIHTFSGIVTRDPETRVMNVGI
                     YRGMIGRKDTAPFLLIKGGQHWGAHFAKWAAHKQPMPVACVIGWDPIMPFLAGSPVLA
                     GVCEWDVMGAYRGEPAQLVRCETVDLEVPATAEIVIEGYISDDPATYESEGPFGEFTG
                     YVSDLATPRPTMNVTCITHRRDPIFCGSLEGTLPGSYSENSVMSSVQRASIAWNILNT
                     AGIPGVLDVYVPPITNGVNIRIQIKKHYQGQPKQIAAALWGNSAAQYRYKHVIVVEED
                     IDPADDEQVEWALAHRLNAGEGGVVIFPGIFGSPIDPSTPMEDRDVAQLGTGLWNRML
                     IDATRSWKNPRRSEWGGERFPPTVRPSPEDEARVRARWAEYGLGDL"
     gene            9139..10110
                     /locus_tag="VM910_24070"
     CDS             9139..10110
                     /locus_tag="VM910_24070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65631.1"
                     /translation="MKHLAGLALSLGIALAPSMLLAQDSWPARPITFVVPYGAGGYTD
                     LVGRLTARYVEKALGKPVIVDNRAGAGGIVGTQAIANAAVDGYMFCVCSIGAISIAPF
                     DPNQKVGYDPLRDLAPVGIVSSISQVVIVNKDLPVKTLAEFVSYAKANPSKLNYGSSG
                     AGGLTHYSVELFQARTGIKVVHIPFKGGAPSTAAVVSGEVDFSFANITDALPQVQAGS
                     VRGLAVTSLERSPHLPDLPTVHEAVLPDFVVETWNGIMAPSKTPEPIIRRLSETLIMM
                     ADDPEVKDIMRKAGADTVKTTPEQYRAQIVREIAQWKPLIQEIAERK"
     gene            complement(10147..>10329)
                     /locus_tag="VM910_24075"
     CDS             complement(10147..>10329)
                     /locus_tag="VM910_24075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65632.1"
                     /translation="AIDHHGITPMRFRLFIIGQLERSAIMRGLLRRPTVGFNAALNGT
                     AFSFLRKRKTPVTRLN"
BASE COUNT         1915 a         3198 c         3133 g         2083 t
ORIGIN      
        1 ccacgcgtgg tgccaagtct atctgccggg cgccggctgg gtcgaatttg atccgacaaa
       61 cggcatcatt ggaaatcgtg acctcatccg cgtcgctgtc gcgcgtgacc ccagccaagc
      121 agttccgctc tccggcacct atgttggaaa ttcggaggac gagttaggca tgaaggttga
      181 agtcagcgta aattgtgagt ctggtccccg taatcgtcaa tagcagcggc acatcacttg
      241 ggctcatctc atcactaatc cgggtcccga cagggctgat ggctcagttc tcccgccgat
      301 cctttcatgt acttgcgacg acgaccgtac tggcggctga ccgagcacga ggatgggtac
      361 cgtctcgctt tatccttccg tctggcgcat tagccggggt cgcgtggagt ggtgtagagc
      421 agttaccgga tagtagccat ttcatgtccg tgcgtcgggc ctacttttgg caaaccagaa
      481 gacgaagagg agggagggac cgccatgaag aaccggcggt cgtcagagaa acccgacgaa
      541 tacccgcagg cgcgtcaact tttgcccccc tcttcttcgg gctggtacat ggacgtcttg
      601 tgcacgacca cgccgctcgg ggtcctcaat tcacgcggtg atttgggaat cccgtcgcgc
      661 ctacattcgg caagattggt aaactccttg ccttaatgcc gcagtaacga gaagaggagg
      721 gcgagcgcaa ggagcgcggc caggccctgc cagaatagga gtgggtagcg ttcatagagc
      781 gttcgtggct tgcggaccgc aagcggggcc cgcgcgggtc ccgtctccat ctccacggca
      841 aattcgagca tgtcgcgaaa acgttcggtg ggatcgattg cgattgctcg cccgagtgca
      901 gcctggagcc aggctgggag gtccgggcgc agggcggaaa gcgctgtcgg ccggtttcgg
      961 cgcggtgggc tggtggcatc gcgattgccg taagggaact cgctggtgaa ggcgcggaac
     1021 atggtgacgc ccagggcgta aatgtcggta gcctcgttcc cgggttctcc tgccagcatt
     1081 tcgggcgcca tatagccgag ggtgcccgga atgtcctcgg gcgggaaatc ctccagtccg
     1141 ggcacacgga ccacgccaag atcgatcagc ttgagcgatt cgtcgccctc gaggatcacg
     1201 ttgtccggct tgatgtcgcg atggatgatg ccggcgcggt gtagcgccgc cgcaccgcgg
     1261 gccaatttga ttgcgatgtt ccgtccttcc tccagaccca ccgacgggct gcgtgcgagc
     1321 cgcgtagcca aaagctcacc tggatacagc ggcatgacgg tatacaggca ggtctgtcgc
     1381 ccaggcggca actcgattgt gcgaccaacc caagggctgt gcacgcgggc gcccacccat
     1441 gcctcacgga cgaatgccgc gcggtaggtg gccacggatg cgacctgcgg cttgggaaac
     1501 ttcaacacca cttcgccgcc ctcgacctcg tcaaccgcac cgaacaggcg ggcgtatcgg
     1561 ccgtcggaca ccagcacttt gagcacaaag ccatcgatcg tctctccggc gacgggcacc
     1621 gggatcagcg gcagttgcat gatggcggcg ccgatatcgg ccgattccgc cgtggggacc
     1681 tcaaccacgt cgagtaccaa ggccgtgcag ttatcggtac tgccagagcg gagcgcggct
     1741 gccacaagcg cacgggcggt atcctcggac gcagagcgct cgcgcagaat gtcagcgatg
     1801 gtctccgccg tcaggatccc gtgcacaccg tcgctgcaca ggacgaagcg atcgtgccgc
     1861 gccatcggct gcgccgcata gtccagccgc acctccgtct cgacgcccag cgcgcggtta
     1921 agaatgctcg accgaccagt gccaccctcg cgcacatggt cgatggtcag gctggtgagg
     1981 cggtcgccgc cgagccgata ggcgcgcgtg tcgccgacat gcagcacgtg cgcgacgcgc
     2041 ccgcgcagta ccagcgcggt gaaggtgcat cccatccccg ccaacttggc gtcgcgctga
     2101 ccttgcgaat agatccagcc attgagcgca ttgaggaccc tggccgccgc acgccgcact
     2161 tccatggtct cggggaggtc gcaaaatcca tccagaaaac cacgcaccgc ggtctctgct
     2221 gccacgcgac cgccctttgc gcccccaatc ccatcggcga tcgccgccac gacgtcgcgg
     2281 cgcggctctg gcagctccaa gccgaaaacg gcgccggcga aatcctcatt gcgttgacgc
     2341 ggtccagtct ccgaggcgaa ccccacgctt gccttgacgc cggagagccg ctcaaccatc
     2401 cccactgctc cgattttcgg ggcgcagcgg gcagccagga tcgtgacgcg gtaccagcgc
     2461 acacttcaat tccgcgatcg cggcttcaat tctccagcct gcaccgatca tagaggaaat
     2521 agccgccggt gcttgacgcg acaattaccg tcagatttcc ggatttgcga tcaagcgtgt
     2581 agttgccgcc gttcgttgcg tcgtgccacg taatcttgga gtcggtgaca cgggccggat
     2641 tggaatccac ggtgctccga tcgtagtcga tcctaatctg ccagcttgca ccgctaaccg
     2701 gattggtgca ggtgatcgcg gtctcgccgc gctgcgcttc accgttaatg gcgggaaagg
     2761 ccataccgat cgtcaaggcg ccgattcccg ccactagtga ccgagtcaga accaatagtc
     2821 gcacactcgc ctgcccactt tataatcaaa atgcagactc tttagtcatc ttgaaccagg
     2881 ctcagcgaca accttcatga gaaaatttgc tcagtcaatg ctcaattagt tggcataatt
     2941 tttgcgatac ccagttgtcc ggctgctggc gcgcagaggc ttttggggga gagacatggc
     3001 actcgtacca tcaccacaat ggctcgatac cggaaacaac gcctggcagt tggcggccgc
     3061 taccttcgtc ggcctgcaaa gcgttccagg gctgacgatt ctctatggtg gcatcgtcaa
     3121 gaagaaatgg gcgatcaact ccgccttcat ggcgatgtac gctttcgcgt cggtcctcgt
     3181 cgtctgggtt cttttcgcct acaacatggc gttcgggccc caatggttcc cgttcctcgg
     3241 gacgccgggc ttggcgacgt cggcgatgtt taccaccggc caagcgacag ttccggctgc
     3301 cgcatcgggc atgccgcccc tcacctttcc aatggcgacc ctgatattct tccagttcgt
     3361 attcgctgcc atcactgtca tcatcctcgg aggttccctg cttggtcgca tgaacttcac
     3421 ggcgtggatg atcttctgcc cggtgtggac gaccctggtc tatacggtgg gggcgttcag
     3481 cctatggggt ggcgggtggc tcgccggcat gggcgtggca gatttctcgg gcggctatgt
     3541 catccatctt gccgccggaa cctccggatt cgtcgctgcg gcgatggtcg gcccgcgcct
     3601 gcaacaggat cgcgaccatt tcccgcccaa tagtttgttg gtcacgttgg ccggtgccgg
     3661 aatcctgtgg ctgggttgga acggcttcaa cggtggcgat ccctatttcg ccaacgcgga
     3721 tgcgggcgcg gcagtgctca acaccaatac tgccaccgca acggcccttc tggtctggac
     3781 gctgatggac aagatggcct atggcaagcc gtcggtgatc ggcgccgtca acggcatgat
     3841 cgcgggcttg gtggcaatca ctcccgccgc cggctacgtg gatggctggg gcgctatcat
     3901 catcggcgtg gccgctggca tcatcccgtg ggccagcatg aacaagctgc aaaaaaccaa
     3961 gctgatgatg aaggcggacg atacgttgag cgtcctttcc acacatggtg ttgctggctt
     4021 gaccggcggt ctgctcgtgg gcatcctcgg caatccggat atgctccaat acatcggcac
     4081 tgaaaaggaa gcgccgggcg tggccgtgac gggctggctc tacggtaatg ccccacagct
     4141 cctgcttcag gcggagggag cggcgttcat tatcgtatac aacgtgatag cgacgttcat
     4201 catcctcaag atcatctctt tcatcgtgcc actgcgtatg gatgacgcaa ccctgaaggt
     4261 gggagacgac gcggttcacg gcgagacggc ctatgcgatt ggcgtcgagg gcgagtagtc
     4321 ggtcccgccc tgatggattg cctcaaggct tcactgccgg ggttgttttt gtggaggtac
     4381 caaaaagaga ctccgcttcc tgttgaatcg cgtttgtctg ccccgtggcg acaattgctc
     4441 acttgcgagc agatggccgc tcgcaagttt ggggtccgcg ctcaactcct gtcgcgcgga
     4501 cccttggcgg ccccaaccac tcaagggggg ccgccctttt ttcttatcgc tgctgggaca
     4561 aacgccgcga attttcgtgt gattgctaaa gcgcaatgaa tgcgcatttc gtgccgagct
     4621 tgatcgcggc atggccgcgg cggactatga ggtggccagc gcgcttttta ccaatgccct
     4681 atctggcaat gtcgcggctc tacatcgacc gcatcctgcg cggcgaaagt cctgccgacc
     4741 ttccgatgca agcacccacc aagttcgagc tggtgatcaa tctgcgaacc gcgaggacgc
     4801 tcggcctcga cgtgccaccg tcgctgctcg cccgcgccga cgacgtgatc gagtgagaat
     4861 gcttggttgt tgctgcggtg cttgaccttt gtcgcggccc ctcatggtcg tgcggcgtcg
     4921 tttctgttag gctgctgatc tggcataatg cagggggcaa accatgcgtt cgaaactcac
     4981 cacgattatt ctcatcggta tggtgctggg tattctcgtc ggttacgcct gtcacattct
     5041 gtggccggat ccgaaaacgg caaagactat cgccgattac atctccctgt tcaccgacat
     5101 tttcctgcga cttattaaaa tgatcatcgc gccgctcgtg ttctcgaccc tcgtggttgg
     5161 cgtcgcgcat atgggcgaca cgaaggcggt tggccgcatc ggcgccaagg cgatggtctg
     5221 gttcatcggt gcgtcgcttg tatcgctcct ggtcggcatg atcctggtca acgtgctcag
     5281 gccgggcgac aatctcaatc tcccgctccc cgacgcggag gcttcgacca acctcaaggt
     5341 tgcgtcattg tcgatgaagg agttcgtcgc gcacctcgtg ccgcgttcgg tcgtcgaggc
     5401 gatggcgacg aacgaaatcc tgcaaatcgt cgtattctcg ctgttcttcg gtgtcgcctg
     5461 cgcggcgctc ggcgagcgca cccgcaagct ggtcgaaggc atcgagcagc tttcctatgt
     5521 catcctgtcg atcaccggtt acgtcatggc gctggcgccg atcgcagtgt tcgcgtcgat
     5581 ggccgcgatc gtgacgaccc aaggcctcgg catcctcgtg acctacggca agttcgtcat
     5641 cgagttttac ttcggacttg ccgtgctgtg gtgcctgctg atcgccgtcg gcttcctgat
     5701 ttttggccac catagcttcc ggctcgtcaa tctgatccgc gagccgtttc tgctcgcgtt
     5761 ctccaccgcg agcagcgagg cagcctatcc caagaccatg gagcagctcg aaaagttccc
     5821 catcagcaag aagatcatca gcttcgttct gccgatgggc tattcgttca atctcgacgg
     5881 ttccatgatg tactgcacgt tcgcaacgct attcatcgcg caggcctaca atatcgagct
     5941 gccgctcgga cagcagatta cgatgctctt gcttctgatg ctaacgagca agggcatggc
     6001 gggcgtgccg cgcgcttcgc tcgtggtcat cgctgcgacg cttgccagct ttaacattcc
     6061 cgagtcggga ttgctgctga tcatcgggat cgatcaattc ctcgatatgg gccgctcggc
     6121 aaccaacgtg attggcaaca ccctggccac ggccgtgatc gcgaagtggg agggcgagcg
     6181 ggtagatctc ggggaactgg aggccgcgct cgcacccaag gctgcctgat gcgtgtcatg
     6241 cgagcgagtt gcgcgagcag aagaaccgcc gacgcaggca acggcctggg ctggccgagg
     6301 attgtcatta ccgccgcgtt tgtcctcatc ctcgctgcac cggcaaatcc ccaggaggcg
     6361 gcaaatgttg gggcgctctc cgggacactc aagaaggtca aggacaccgt cacgattaca
     6421 ctgggctacc gcgagagctc gctgcccttc tcctatctga atcgaaggca acaaccgatc
     6481 ggctactcga tcgatttgtg ccgcgagatt gtggaagacg tctctaccga gctcgacggc
     6541 atggagctca agatcgcatt cgccccggtg acgccggcaa atcgcctgca gaaggtcgca
     6601 tccggggaga tcgatctcga atgtggctcc actacgggca atgtccaacg ccggaaggaa
     6661 gtagcatttt cgccgatttt tttcgtcgct ggcaccaagc tgatggtgcc gaggtcctct
     6721 tcggtccgct cctaccgtga ccttgcgggc aagaccgcgg tcgtgaccgc cggcacgacc
     6781 aacgaggcgg cgctacgcac gctctcggac aagcagaagc tcagcatcac aattgtcacc
     6841 gcccccgatc acgcgcaatc gctcgcgaca ctcgtatccg gcaaggcgga tgcgtttgcg
     6901 accgacgacg tgcttctgta cggcttcatt gcgacggcaa aggacgcgcg cgacatgaag
     6961 gtcgtcggcg agtatctgtc gtacgatccc tatggcctag tttatcggcg cgacgatccg
     7021 gctttcgccg cggtggtcga gcgcgctttc acgcgaatgg caagcgagcg acggctcgcc
     7081 gagctctaca acaagtggtt cctgcgccgg ttgccgaccg gtgagacgtt gaatttaccg
     7141 atgagcccgc aactggaaga ggttttccgt atgctggggc agcccgaata gagatcgtct
     7201 ctgcggctcc gatggtgtcg gcatgtgcca ccgagctcac ttagcagatc ggagtgactt
     7261 ctgcgccttt ggcacgatgc ggaagtgacg aatccagagc tccatctttg caattaacga
     7321 tgtcggcgcc tcaagagcag gcgtcgtcgc tcggtcttat tgctttcatc gcggcggagc
     7381 cgccgggaga gcagcgctct cgcgcaggtc cgtcacaggt cgccaaggcc gtattccgcc
     7441 cagcgggctc gcacgcgcgc ctcgtcttcg ggggatggtc gcacagtggg cggaaagcgt
     7501 tcaccgcccc actccgagcg gcgcggattc ttccaggagc gcgtggcgtc gatcaacatc
     7561 cggttccaca ggccggtgcc gagctgcgca acgtcgcgat cctccatggg cgtgctgggg
     7621 tcaatgggcg agccaaaaat cccagggaaa atcaccacac cgccttcgcc ggcgttgagc
     7681 cggtgagcga gcgcccattc aacctgctca tcgtccgcgg gatcgatgtc ctcttccacc
     7741 acaatcacgt gcttgtagcg gtattgcgcg gcgctgttgc cccagagcgc tgccgcgatc
     7801 tgcttaggct gcccctgata gtgcttcttg atctggatgc ggatgttgac gccgttggtg
     7861 atgggcggca cgtagacgtc gaggacgccg ggaatgcccg cagtgttcag aatgttccag
     7921 gcgatgctcg cgcgctgcac cgaggacata acgctgttct cgctgtagga ccccggcagc
     7981 gtgccttcga gcgaaccgca gaagattggg tcgcggcggt gagtgatgca cgtgacgttc
     8041 atggtcggac gcggcgtggc caggtcggac acgtagccgg tgaactcacc gaacggccct
     8101 tcgctttcgt aggttgccgg atcgtcgctg atgtagccct cgatgacgat ctctgcggtc
     8161 gctggaacct cgaggtcgac cgtttcgcag cgcaccagct gcgccggctc gccccggtag
     8221 gcgcccatca catcccactc gcacaccccg gccaacaccg gcgatcccgc caagaacggc
     8281 atgatgggat cccagccgat cacgcacgcc accggcatgg gctgcttgtg agcggcccat
     8341 ttcgcaaaat gtgcccccca gtgctggcca cccttgatga ggaggaacgg cgcggtatcc
     8401 ttgcggccga tcatcccgcg atagataccc acattcatca cacgcgtctc cgggtccctt
     8461 gtcacgatgc cggagaaggt gtggatgtaa cgcccgcctt ccaggtactg ccactgaggg
     8521 acaggaaact cggtctgatc gatggcctcg ccgcgcacga tgatgtcctt caccggcccc
     8581 gtttcgacga tgatcggagc gaccgtctcg cggtttttct tcatcacgtg ctggatgagt
     8641 tcccgattgg cgacatcgcg gggaaagccg agagccaggg ccaggcgcga ttgtgcgccc
     8701 aggccgctca caaaaagttt ggtacagcgt ccactcctgt agcccttgat gccctcgaac
     8761 agcagcgcag gccccttctt ctcaagcacg cgccgagcaa tggctccgat ctcgcggtgc
     8821 cagtccacct cggcggtgat ccgccgcagc tcgccctcct tctcaagaag gccaatccac
     8881 tgccggacgt ccatgaattt catcctgtgc tcactcccaa atcttgccgg ctcgccgcgt
     8941 cctcagttcg cttctgagca aggaataggc aagcaagcgc ttgaggaaac cggttaggat
     9001 cgccgcatca agtaactaac acggcagagc gtgaacgtat aagcgcagag gcgccggcta
     9061 accgactgag attccaggac ttcgagcaag cggcaaagaa attgcttctt gcgccaacaa
     9121 agacagcagg aggatgcgat gaagcatctt gcaggactgg cattgtcgct ggggatcgcg
     9181 ttggcgccga gcatgctgct agcgcaggat tcatggccgg cccggcccat cacgttcgtc
     9241 gtgccctatg gagcgggcgg gtatacggac ttggttggcc ggctgaccgc gcgttacgtc
     9301 gagaaggcct tgggcaagcc ggtgattgtt gacaatcgcg cgggcgccgg cggcatcgtg
     9361 gggacgcagg cgatcgcgaa tgcggcagtc gacggctata tgttctgcgt ctgcagcatc
     9421 ggcgccatct cgatcgcgcc cttcgaccca aaccagaagg tgggctacga tccgttgcgg
     9481 gaccttgcgc ccgtcggcat cgtcagctcg atctcgcagg tggtcatcgt caacaaggac
     9541 ctgccggtga agacgctggc cgagttcgtg tcctatgcca aggctaatcc cagcaagctc
     9601 aactacggct ccagcggcgc cggcggcctg acccattact cggtcgagct gttccaggcc
     9661 cgcaccggta tcaaggtcgt gcacatcccg ttcaagggcg gtgcgccctc gacggccgcg
     9721 gtggtctccg gcgaggtcga tttctccttc gccaacatca ctgacgctct gccgcaggtc
     9781 caagccggat cggtgcgcgg cctagccgtg acctcgctcg aacgtagccc ccacttgccc
     9841 gatcttccca ccgtgcatga ggcagtcctt cccgattttg ttgtcgagac ttggaacggc
     9901 atcatggccc catcgaagac cccggaaccg atcatccgca ggttgtcgga gaccctgatc
     9961 atgatggccg acgatccaga ggtgaaggac atcatgcgca aggccggtgc cgacacagtc
    10021 aagaccacgc ccgagcagta ccgtgcgcag atcgtccggg aaattgcaca gtggaaaccg
    10081 ctcattcagg agatcgcgga gaggaaatag ccgctgctga gcttggcgtg cgaaggctcg
    10141 cctttctcag ttgaggcgcg taaccggggt cttacgtttc cgcaaaaagg agaaagccgt
    10201 gccgttgagc gccgcgttga aaccgactgt tggtcggcgc agtagacccc gcatgattgc
    10261 ggagcgctcg agttgaccta tgatgaagag acggaaccgc atgggagtga tgccatgatg
    10321 atcgattgc
//
LOCUS       DATLJT010001047         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_759290,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001047 DATLJT010000000
VERSION     DATLJT010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759290"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..781
                     /locus_tag="VM910_24080"
     CDS             80..781
                     /locus_tag="VM910_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI65633.1"
                     /translation="MRVLLIEDDSAVAQSIELMLKSESFNVYTTDLGEEGVDLGKLYD
                     YDIILLDLNLPDMSGYDVLKQLRVSKIKTPILILSGLAGIEDKVKGLGVGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVIQTGDLVVNLDTKTVEVGGQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLANASEGRNFIETVWGRG
                     YVLREPHDLDERIPA"
     gene            933..1337
                     /locus_tag="VM910_24085"
     CDS             933..1337
                     /locus_tag="VM910_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HVI65634.1"
                     /translation="MILQSLAGLPAFLVYFCTAIIAVVAYLLVYTRITPHNEFQLIRD
                     NDPAAAIALGLSLLGFTLPVVSAIAHAANVVDCLIWSAIALIVQVAVYYLVKIPVPNL
                     SQRIAAGELAPAIWLGLSSLAAGALNAASMIY"
     gene            1340..1741
                     /locus_tag="VM910_24090"
     CDS             1340..1741
                     /locus_tag="VM910_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65635.1"
                     /translation="MTDMRPTGKAMEFGKRRPTLPAQPAPKRSGHVALLVMGTLAVGG
                     GAYALMPGESCAPASAIPGMAAPTIPQTTACTSRGGSGGYGGYAGSSRYGLYGDSSNR
                     GSAAGSTDTSSGGVTRGGFGAFARAFGFSGG"
     gene            1852..2082
                     /locus_tag="VM910_24095"
     CDS             1852..2082
                     /locus_tag="VM910_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65636.1"
                     /translation="MKRSRRVVLTMMGSAAAGAVSLGFVPYGDCGAGRMAVRSQDGRI
                     VCRATYGGFGGTLHHFHGHAHGHGHGHGHAGG"
     gene            2091..>2932
                     /locus_tag="VM910_24100"
     CDS             2091..>2932
                     /locus_tag="VM910_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HVI65637.1"
                     /translation="MQRIACSERDDWRETSEACGFDFHTIDGERYWDERAYYAFSLDE
                     IEQKIEAPTGEIDAMCLDLVGRAIRNEDYLKRLKIPETFWPLIAESWERDERSLYGRL
                     DLSFDGSGPAKLLEYNADTPTSLFESAVFQWTWLEQAMERRIIPNAADQYNSIHERLI
                     ATWNELGQGRHLHLTGTTGSEEDLGTLTYLEDTAIQAGLTTTIIDIEDIGWRDEGGFV
                     DLTGRDIELVFKLYPYEWMFRDAFGVRLKDAPTRWIEPPWKAVLSNKGILPLLWEMFP
                     GHPNL"
BASE COUNT          553 a          906 c          904 g          569 t
ORIGIN      
        1 agttgttcaa aggcgttttg agtgccgcaa gtgctgcggc ccgacctgac gcccggagcg
       61 gcgactatgg ggaactggca tgcgcgtttt gctgattgaa gatgacagcg ccgtcgcgca
      121 gtcgatcgaa ctaatgctca agtccgagag cttcaacgtc tacacgaccg atttggggga
      181 agaaggcgtc gatctcggca aactctacga ttacgacatt atcctcctcg acctgaacct
      241 gcccgacatg tccggctacg acgtgctcaa gcagctcagg gtctccaaga tcaagacccc
      301 gatcctgatc ctctccggcc tcgccgggat cgaagacaag gtcaaaggtc tcggcgtcgg
      361 cgccgacgac tacatgacca aacccttcca caaggacgaa ctggtcgccc gaatccatgc
      421 gatcgtgcgt cgttccaagg gccacgccca gtcggtgatc cagaccggcg atctcgtggt
      481 caatctcgac acaaagacgg tcgaggtcgg gggtcagcgc gtgcatctga ccggcaagga
      541 atatcagatg ctggaactgc tctcgcttcg caagggcacc acgctgacca aggaaatgtt
      601 cctcaaccac ctctatggcg gcatggacga gcccgagttg aagatcatcg acgtcttcat
      661 ctgcaagctg cgcaagaagc tcgccaacgc ctccgaaggc cgcaacttca tcgaaaccgt
      721 ctggggtcgc ggctatgtgc tgcgcgagcc gcacgacctc gacgagcgta ttccggcctg
      781 atcaggtttc gcgcggcctg aacggctcgc tcctcccgaa ctggaccccg ccgcaaatgg
      841 cggggttttt gtttggggaa tgaattgtca atcgcggtgc atcgactgag agatcgtgcg
      901 aattctggta tcccaccgat cggggcgccc tcatgattct ccaatctctt gccgggcttc
      961 cggctttcct ggtctatttc tgcaccgcca tcatcgcggt cgtggcttac ctgctggtct
     1021 atacccgcat cacgccgcat aacgagttcc aactgatccg cgacaacgat ccggcggccg
     1081 cgatcgcgct gggcctgagt ctgctcggct tcacgctccc agtcgtcagc gcgattgcgc
     1141 atgcggccaa cgtcgtggac tgcctgatct ggagtgcgat cgcgttgatc gtccaggtgg
     1201 ccgtctatta tctggtcaag attcccgtgc cgaatctgtc acagcggatc gccgccggcg
     1261 agctcgcacc cgcaatctgg ctcgggctgt cgtcgcttgc tgccggtgcg ctgaacgccg
     1321 cctcgatgat ctattgatca tgacggacat gcggccgacc ggaaaagcaa tggagttcgg
     1381 caagcgccgg ccgacgctgc ctgcgcaacc ggcgccaaaa cgctccggcc atgttgccct
     1441 gcttgtgatg gggacgcttg cggtcggcgg cggcgcctat gcattgatgc cgggcgagag
     1501 ctgcgcgcct gcatctgcca tacccggaat ggcggcgccg acgatcccgc aaacgaccgc
     1561 ctgcacctcg cgtggcggga gcggcggcta tggcgggtat gctggctcat cgcgctacgg
     1621 cctttatggc gattcttcga accgcggatc ggcagccgga tcgaccgaca cctcgtcggg
     1681 cggcgtcacc cgcggcggct tcggcgcgtt tgcgcgcgcc ttcgggttca gtggcgggta
     1741 gacgtcggcg acaaattatt tttgccgctc aatgtcgtga aatcaccgcc tttgacttgc
     1801 gtgatcgtcg tcggctcagc agaatcgaag agcggaagga ggccgcgacc catgaagcgt
     1861 tcgcgccgtg tggtgctgac aatgatggga agtgcagcgg ccggcgccgt ctcgctgggc
     1921 tttgtacctt acggcgactg cggcgcaggg cggatggcag tgcgctcgca ggatggccgc
     1981 atcgtctgcc gcgcgacata tggcggcttc ggcggtacgc tgcatcattt ccatggccac
     2041 gcccacggtc acgggcatgg gcatgggcac gcgggcggtt gaatccttcc atgcagcgca
     2101 tcgcctgctc cgagcgcgac gattggcgtg agacctcgga ggcctgtggc ttcgatttcc
     2161 acaccattga cggcgagcgc tattgggacg aacgcgccta ttatgccttc tcgctggacg
     2221 agatcgaaca aaagatcgaa gcaccgaccg gcgagatcga cgcgatgtgc cttgacctcg
     2281 tcggccgcgc gatccgcaat gaagactatt tgaaacgact gaagatcccc gaaacgttct
     2341 ggccgctgat cgccgagagc tgggagcgcg acgaacgaag cctctacgga cggctggact
     2401 tgagcttcga cggtagcggg ccggcgaagc ttttggagta caacgccgat acgccgacct
     2461 cgctcttcga atcggcggtg tttcagtgga cctggctcga gcaggcgatg gagcggcgca
     2521 tcatcccgaa cgcagccgat cagtacaatt cgatccacga acggttgatc gcaacctgga
     2581 acgagctcgg ccagggccgg catctgcatc tgaccggcac caccggaagc gaggaggatt
     2641 tgggtacgct cacttatctc gaggacactg cgatccaggc gggccttacg accacgatca
     2701 tcgatatcga ggacatcggc tggcgcgacg agggcggctt cgtggacctc acaggccgcg
     2761 acatcgaact cgtcttcaag ctctaccctt acgaatggat gttccgcgac gcgtttggcg
     2821 tgaggttgaa ggacgcgccg acacgctgga ttgagccgcc ctggaaggcg gtgctctcca
     2881 acaagggcat ccttccactg ctgtgggaaa tgtttcccgg gcaccccaat ct
//
LOCUS       DATLJT010001048         1681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3059591,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001048 DATLJT010000000
VERSION     DATLJT010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3059591"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VM910_24105"
     CDS             complement(<1..1255)
                     /locus_tag="VM910_24105"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI65638.1"
                     /translation="MSKRRNFPDSNRRNFLKGAGLASAAALTPLAAKAQVSAPKQKPK
                     ASVPGPTEIAVETLPPSKDPVNQTSSGGDFMVDVLKTLNFDYLTMNCASAFRGIHESF
                     INYGKNTKPEILTCPHEEIAVHMAQGYAKMEGKPMAMMCHGTVGLQHASMALYNAWCD
                     RVPVYVMIGNIIEGDKRAPGVEWVHSAIDPAAIVRDFVKWDDQPASLQHFAESAVRAY
                     KIAMTPPMAPVLLSLDAELQENPIVDPESLRIPKFANVVPPVGDAGALGDLAKMLVTA
                     ENPVIICDRLARSPAGMSRLVELAETLQCAVVDNAGRMNFPSRHPLNQSFRRSVISQA
                     DVILAIEMNDVFGSLNAFSDRIARATRPTVKKGAKIITLGLRDTYMKSNYQDFGRYQD
                     VDLAIAGDGEASLALLIEAVKRLIDP"
     gene            complement(1501..>1681)
                     /locus_tag="VM910_24110"
     CDS             complement(1501..>1681)
                     /locus_tag="VM910_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI65639.1"
                     /translation="ATAPDGTRLAGPVPAKLPVGIMFIGKPFSEPILLRIASAYEAGT
                     KHRIPPPDFGPVAGP"
BASE COUNT          314 a          518 c          510 g          339 t
ORIGIN      
        1 acggatcgat taagcgtttc accgcctcga tcagaagcgc aaggcttgcc tcaccgtcac
       61 ccgcaatcgc gaggtcgaca tcctgatagc gtccgaagtc ctgataattg gacttcatat
      121 acgtgtcgcg gagccctaac gtgatgattt tggcgccttt cttgacggtg ggtcgcgtcg
      181 cccgagcgat gcgatcgctg aacgcgttca gacttccaaa tacgtcgttc atctcgatcg
      241 cgagaatcac gtcggcttgc gaaataacgg atctgcggaa cgactgattg agtggatgac
      301 gcgagggaaa attcatccgg ccggcattgt cgaccaccgc gcattgcagc gtctcggcta
      361 gctcgacgag acgcgacatg ccggctgggc tccgcgcgag ccggtcgcaa atgatcacgg
      421 gattctcggc tgtcaccagc atctttgcca ggtccccgag cgcgcccgcg tctcccacgg
      481 gaggcacgac attggcgaac ttcgggatgc gcagactttc tgggtcgacg atcgggtttt
      541 cttgcagctc agcgtcgagc gagagcaaga ccggtgccat tggcggtgtc atcgcgatct
      601 tgtaggcgcg cactgccgac tcagcaaagt gctggagaga cgccggctgg tcgtcccatt
      661 tgacgaaatc gcgcacgatc gcggccgggt caatcgctga gtgcacccat tcaacgccgg
      721 gtgctcgctt gtccccctcg attatgttgc cgatcatgac atagaccggc acccggtcgc
      781 accatgcgtt atacaacgcc atgctcgcgt gctgcaggcc cacggtgccg tggcacatca
      841 tcgccatcgg tttgccttcc atcttggcgt agccctgcgc catatggacg gcgatctctt
      901 catgaggaca ggtgaggatt tcgggcttgg tgttcttgcc gtaattgatg aaggactcat
      961 ggatgccgcg gaacgccgaa gcgcagttca tcgtcagata gtcgaaattg agcgtcttga
     1021 gcacatcgac catgaaatcg ccgccgctcg atgtctgatt caccgggtcc ttcgagggcg
     1081 gcaacgtctc gaccgcgatc tccgtcggcc cgggaacgga agccttgggc ttttgcttgg
     1141 gcgcggaaac ctgcgctttg gccgcaagcg gcgttagcgc cgcggcgctg gcgaggccgg
     1201 cccccttgag aaagttgcgg cgatttgaat caggaaaatt gcggcgtttc gacatgcatt
     1261 cctcccagcg atggggtcga cgcgggttca gcaatttgcg caaagtcagc gcatcagtct
     1321 cgcgccgccc cctgcccccg tcaaggcacg gcgccatccg gtggcagccg catgcgctgc
     1381 tgcgcgcgcg ccacgagcgg tcatcccgtc gccgcgccgc cgacgagcgt gatgaactcg
     1441 cggcgcttca tcgctcgatc acctcgtcgg cgatggcgag cacgatatgc cgaccaccct
     1501 ctacggtcct gcaacaggac cgaagtccgg cggcggaata cggtgttttg ttcccgcctc
     1561 gtatgccgaa gcgatccgca agaggatcgg ttcggaaaac ggctttccga taaacataat
     1621 tcctaccgga agtttcgccg gaacgggtcc ggccagacgg gtaccgtcag gcgccgtcgc
     1681 a
//
LOCUS       DATLJT010001049         1908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3519407,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001049 DATLJT010000000
VERSION     DATLJT010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519407"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..1404)
                     /locus_tag="VM910_24115"
     CDS             complement(421..1404)
                     /locus_tag="VM910_24115"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65640.1"
                     /translation="MRRRDLITLLGGAAAAWPLAARAQQAGKVWRLGILGPSPNVPNL
                     VAQHQAFLAELKALGFEEGKNFTIDYGRVDDPRGTFVAAAELLRSPVDLIWANGPEVA
                     LQAVVGASRIIPIVILAVNYDPIERGYVSSLAHPGGNITGVFYRQPELAAKQLELLTQ
                     AFPNATRLGVVWDVLSADQFAAAEPVARARQLALQPLKLEKPPYDFAAAFRILAEGGS
                     QMVLVLSSPFFSEHRPQIAEAARAQRLPTMFIFRSYVDAGGLMSYGVDQIPMYRRIAA
                     YVAKILNGAKPADLPVEQASNFKLVVNLKTARAIGVDLPTAILLRADQVIE"
     gene            complement(1401..>1908)
                     /locus_tag="VM910_24120"
     CDS             complement(1401..>1908)
                     /locus_tag="VM910_24120"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65641.1"
                     /translation="PAATRVAVLVNPASAANTEATLRDVQSAAGAMGVQIQVLNASTS
                     REIEATFATFVRERADALFVGSDAFFNNRRVQLAHLATLHKLPTTYSQRQYAEAGGLM
                     SYGPNIADAWRVVGVYAGRVLKGAKPRELPVEQVSKFELIINAQTARMLGLEVPPTLL
                     ARADEVIE"
BASE COUNT          376 a          580 c          590 g          362 t
ORIGIN      
        1 cagcggaatt tgcaccatca gctattgcgt tccagggtat tgattactcg ctcaagccac
       61 cccggaacgc ctgtgcgaga ttggcacggt cgtttcgtcc cagactgatg aggaattcat
      121 ccccggctgc tgcgcgcgcg cagcgagcgg ccacgaagcg gccgcgccgc cgagaagcgt
      181 ggctaagaat tttcgtcgtc ggatgtagct tgccatgtga ccctccggtt gggggtcatt
      241 catgcaatgg agggatcata ccacgcgatt gaaggacgat accacgcttc catcgcgagc
      301 ggtccgcgac agcgacctgc tcaacgtccg ctttgaccca ctttgcggac tcaagtcgga
      361 catctgccga ggtccgagaa gtgccacaag gcgacatatg cagcgcagcc aagaccgtcg
      421 ctattcgatc acttggtcgg cgcgaaggag gatcgcggtt ggcaaatcca caccaatggc
      481 cctggccgtc ttgagattga cgaccagctt gaagtttgag gcttgttcga ccggaagatc
      541 ggcgggtttt gctccgttca aaatcttggc gacataagcg gcgatgcggc gatacatcgg
      601 tatctgatcg actccatagg acatcagccc gccagcatcg acgtaggatc taaagatgaa
      661 catggtgggc aagcgctggg ctcgcgccgc ttccgcgatc tgagggcgat gttcgctgaa
      721 aaagggactg gaaagcacga ggaccatctg cgagcccccc tcggccagta tccggaacgc
      781 cgccgcaaaa tcgtaaggcg gcttctccag cttcagcggc tgcagggcca attgccgggc
      841 tcgcgccacc ggctctgcgg cggcgaactg gtcggccgaa agtacatccc acaccacgcc
      901 cagccgggtc gcgttcggaa aagcctgggt cagcagttcg agctgctttg ccgccaattc
      961 tggctggcga taaaacactc cggtgatatt gccgccggga tgggcaaggc tcgacacata
     1021 gccgcgttcg atcggatcgt aattgaccgc caatatgacg atcggaatga tgcggctcgc
     1081 gcccaccacg gcctgcaatg cgacttcggg cccgttggcc cagatcagat cgaccggcga
     1141 tcgaagcagt tcggcagctg cgacgaaagt cccacgcggg tcgtcgacgc gaccatagtc
     1201 gatggtgaaa ttcttacctt cctcgaaacc cagggccttc aattcggcca agaacgcttg
     1261 gtgctgcgcg accaagttag gcacgttggg gctggggccg agaatgccga gacgccagac
     1321 ctttcccgcc tgctgcgcgc gcgccgcgag cggccacgct gccgccgcgc cgccgagaag
     1381 cgtgattaag tcgcggcgcc tcattcgatc acctcgtcgg cgcgggcgag tagcgtcggg
     1441 ggcacttcaa ggccgagcat ccttgcggtt tgagcgttaa tgatgagctc gaacttgctc
     1501 acttgctcga ccggcagttc cctcggcttc gcacccttga ggacgcgacc ggcatagacg
     1561 ccgacgacac gccacgcatc cgcgatgttg ggtccgtagc tcatcagccc gccggcttcg
     1621 gcatattgac gctgcgaata tgtcgtgggg agtttgtgga gcgtcgccaa gtgggccaat
     1681 tggactcgcc ggttgttgaa aaaagcgtcg ctgccgacaa aaagcgcgtc ggcccgctcg
     1741 cgcacaaaag ttgcgaaggt cgcttcgatt tcgcggctgg tgctggcgtt gaggacctgg
     1801 atttgcactc ccatggcgcc tgcagccgac tgcacgtctc tcaacgtggc ctccgtattt
     1861 gcagcactgg ccggattgac gagcacggca acgcgagtgg ctgcgggc
//
LOCUS       DATLJT010001050         4137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_303254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001050 DATLJT010000000
VERSION     DATLJT010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..900
                     /locus_tag="VM910_24125"
     CDS             577..900
                     /locus_tag="VM910_24125"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI65642.1"
                     /translation="MADPIYALNLFNVADRGEYLAYSRRSAKEVQAHGGRVVALGKFR
                     EAVTGEIKPRSVMILVEWRSKEAFDEYCNDPKIADLHPHRENGACDYIWHLFDKLDDL
                     RPVLK"
     gene            1031..2149
                     /locus_tag="VM910_24130"
     CDS             1031..2149
                     /locus_tag="VM910_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI65643.1"
                     /translation="MKEPAEGAKTDARPAHIIPWRATGIRLLILLLAGFLVAFLAYEW
                     NWWVGAAREQRTNDAYLQADLTPLAAKSPGYVRSVPVQDFQRVKAGDLLVEIVDDDYR
                     ATLAQAQANVDAARTAIENIKQQKVLQQALVKQAEATIEATQADLTRYHLEAVRQQDL
                     LVTKFSGTEQLVEQAVDNEKHTAAVLELNRAQLDQQRQQMNVLDSQEQQAEAALKGQE
                     AARDLAQIALGYTRITAPVDGMVSERRVRPGQYVSVGTEVINLVPLPNVWIVANYKET
                     QMTRIRAGQPARATVDAFPGVVLHGHVDSWSPASGAQFALLPPDNATGNFTKVVQRIP
                     VKIVLDPDPALAYLLRPGMSVITTIDTSVVPADAAAVK"
     gene            2161..3813
                     /locus_tag="VM910_24135"
     CDS             2161..3813
                     /locus_tag="VM910_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65644.1"
                     /translation="MSSAGAGAFPDVPPPQRGAAAAHSLCDPADMAAQAPPVSSNRPL
                     IGVTGVLLGAIISTLAGRITTFGLADVRGAVHAGFDEGAWITTALTVGQMMIGPPSAW
                     LGMVFGPRRVLMISCTIFGISNFLLPFSPDLPTMLTFQFISGLASGTFIPLAIGFVIG
                     GLPPRMVIYGAAAYSLNLELSLNVSAAIEGWFDDNWSWQWIFWDTALLAPFMLICVYF
                     GMPRQAVSRELLGKGDYWGLLFASLGLSLLYAALDQGNRLDWLNSGLINGLLLGGAIL
                     LVAFVVQELTTERPWINLRFVVRGNFPLLTFYIAFFRSVILSTSYIIPQFLITVQNYR
                     ALEVGGALMWIALPQFLVTPVLATILRFVDARVMLALGFTLVGCACFMAGQLTQDWVS
                     DDFLRSQLVQGLGQSIALTSVLWLNLLNLDPRNVLTFGAVLQTARLFGGEIGNAFSQT
                     FIRVREQVYSNLIGLHVTAGSVLTDQRLADYAQAVGARSVGDPQAHARATALLAEAVQ
                     KQAYVLAYIDGFMVLGFATIVALLLMVLLRPPPGQPMLFQRL"
BASE COUNT          772 a         1274 c         1214 g          877 t
ORIGIN      
        1 cgctaatttg gcggcctctt catcagcagg gttcatcggc gcatttccca cttgggattt
       61 ttccagcggg cagacgttgc cttcatcgac ctcgatttat tagtaggccc tagggccacg
      121 ttcgttcaag gcaagtgggc catttgacat tcttagcaac cctaccactt cacttggcgt
      181 catgcttgca tcagtaaagc taggcgagac cttcccatta aatgaagctt tacgccctct
      241 gacttgaaat ggcgcggtta catcgaggac ttgcgatcgg cgaaattggg atttagggct
      301 cagtttgcac agcagcaatc ctgagccgct cgatgtccgc tttgggtcag aagcggacat
      361 tgaagcgcct ccatccgatg tccgctttac ctccactagc ggacatcgga actcaggtgg
      421 aatgtccggt ttgtgctgaa cccaccactg ccggatggtg tcgctcagcg acgtgcagac
      481 accggccggc tggacgaaga tccaggtaat ccgggacctc agcgtcaccg cactggactc
      541 agccagctgc cgatacaata caagccggag gatctgatgg ccgatcccat atatgcactc
      601 aatcttttca acgttgcaga ccggggtgag tatctggcct actcgcgccg ttcagcaaag
      661 gaggtgcagg cccatggcgg acgcgtcgtt gcgctcggca aattccgaga ggcggtgaca
      721 ggggagatca agccgcgctc cgtaatgatc ctagtcgaat ggaggtcaaa ggaggccttt
      781 gacgagtact gcaacgatcc aaaaatcgcg gatctccacc cacatcgcga gaacggtgcc
      841 tgcgattaca tctggcatct attcgataag cttgacgacc tgaggccggt ccttaaatag
      901 caactgcgtg cacgtccgag gaggcaaagt gattttgcaa ttcaaggacg agacaatcgc
      961 ttctgcacaa gctaattcgg gcccagcggt ggaagcccaa gaaagggtaa ctctggaacc
     1021 ggaagcgatc gtgaaggaac ccgccgaggg cgccaagacg gacgcccgcc ccgcacatat
     1081 cattccatgg cgcgcgacag gcatccggct tcttattctt ctgctcgccg gattcctcgt
     1141 cgccttcctc gcctatgaat ggaactggtg ggtcggcgcc gcgagggagc agaggaccaa
     1201 cgatgcctat ctccaggccg accttacccc gctcgctgcc aagagccccg gctatgtccg
     1261 cagcgttccg gtgcaagact tccagcgcgt gaaggccggc gatctgctgg tcgagatcgt
     1321 cgacgatgat tatcgcgcca cgctggcgca ggcgcaggcc aatgtcgacg cggcgcgcac
     1381 cgcgattgag aacatcaagc agcagaaggt gttgcagcag gcgctagtca agcaggccga
     1441 agctacgatc gaggcgacgc aggcggatct cacccgttat catcttgaag cggttcgcca
     1501 gcaggacctg ctcgttacga agttttccgg caccgaacaa ctggtcgagc aggccgtcga
     1561 caacgagaag cacacggcgg cggtgctcga gctcaaccgt gcgcagctcg atcagcaacg
     1621 ccagcagatg aacgttctcg acagccagga gcagcaggcc gaggccgcgc tcaagggtca
     1681 ggaagcggcg cgcgatctcg cccagatcgc gctcggctac acccgcatca ccgcgccggt
     1741 cgacggcatg gtcagcgagc gacgggtgcg gccgggccag tatgtcagcg ttggaacgga
     1801 ggtgatcaac ctagtgccgc tacccaatgt ctggatcgtc gccaattaca aggaaacgca
     1861 aatgacccgc atccgtgcgg gccagccggc gcgcgcgacc gtcgatgcat ttcccggcgt
     1921 ggtgttgcac ggccatgtcg acagttggtc gcccgcttcc ggcgcgcagt tcgcgttgct
     1981 gccgcccgat aatgcgaccg gtaatttcac caaagtggtg cagcgtattc cggtgaagat
     2041 cgtgctcgat cccgatccag cgctggccta tctgttgcga cccggcatgt ccgtgattac
     2101 caccatcgat acgtcggtgg tccctgcgga cgccgccgcc gtcaaatagg cgatgcggcc
     2161 gtgagctccg ccggggccgg tgcgtttccc gatgtcccgc caccgcagcg cggcgccgcg
     2221 gcagcgcact ctctctgcga tccggccgat atggcggcgc aggcgccgcc ggtcagttcc
     2281 aatcggccgc tcatcggcgt gaccggcgtg ctgctcgggg cgatcatatc gacgcttgct
     2341 ggccgcatca caacgtttgg tctcgctgac gtgcgcggcg ccgtccatgc cggcttcgac
     2401 gaaggcgcct ggatcacgac cgcacttacg gtcggtcaga tgatgatcgg tccaccatcg
     2461 gcctggctcg ggatggtgtt cggtccgcgg cgcgtgctga tgatctcatg caccatcttc
     2521 gggatttcaa atttcctgct gccgttctcg cccgatctgc ccacgatgct aacgtttcaa
     2581 ttcatcagcg gactcgcctc cggaacgttc attccacttg caatcggttt tgtcattggc
     2641 ggtctgcccc cgcggatggt catttacggc gccgctgcct attcgctcaa cctcgagctc
     2701 tcgctcaacg tttccgccgc tatcgaaggc tggttcgacg acaattggtc gtggcagtgg
     2761 atattctggg acaccgcgct actcgccccg ttcatgctca tctgcgtcta tttcggcatg
     2821 ccacgccagg cggtcagtcg cgagcttttg gggaaaggtg actactgggg gctccttttt
     2881 gcgagcctcg gcctcagcct gctttacgcc gcactcgatc agggcaaccg gctcgactgg
     2941 ctcaattccg gcctgatcaa cggactgctg ctcggcggcg ccatattact ggtcgccttc
     3001 gtcgtgcagg aactgaccac cgagcggccg tggatcaatc tgcgttttgt cgtccgcggc
     3061 aattttccgt tgttgacctt ctacatcgcg ttttttcgca gcgtcatcct atcgacctcg
     3121 tacatcattc cgcaattcct catcaccgtt cagaactatc gcgcgctcga agtcggcggc
     3181 gcgctgatgt ggattgcact gccgcagttc ctggtgacgc cggtgctagc gacaatcctg
     3241 cgcttcgtcg acgcccgagt gatgctggcg ctgggcttta cgcttgtcgg ctgcgcttgc
     3301 ttcatggccg ggcagctcac ccaggactgg gtcagcgacg attttttgcg ctcccagctt
     3361 gtacagggac ttggccagtc gatcgcgcta acttctgtgc tctggttgaa cctactgaat
     3421 ctcgatccga gaaatgtgct gacgttcggc gcggtgctgc agaccgctcg cctgtttggc
     3481 ggagaaattg gcaatgcctt cagtcaaacc ttcatccgag tgcgcgagca ggtctattcg
     3541 aacctcatcg ggctgcatgt caccgccggt tccgtcctga ccgaccagcg gttagcggac
     3601 tacgcgcagg cggtgggagc acgttcggtc ggcgacccgc aggcccatgc gcgcgcgacg
     3661 gcgctgctcg ccgaggcagt gcagaaacag gcctatgtac tcgcctatat cgatggcttc
     3721 atggtgctcg gcttcgcaac catcgtcgcc ctgctgttga tggtgctgct gcggccgcct
     3781 cccggccagc cgatgctgtt ccagcgattg tgaaacggct tggcttagtg cgcgttcgaa
     3841 cctagcgaag caggaggccg tattgcgggg cggggaagcg agccaccggc cggcgtgtgg
     3901 agagcccagg ggtgccgagt cggaaatacg atgtttatga gacatcacag acatcgcggc
     3961 atctccaacc tatgtccgct ttacccccaa aagcgcacat tccgagctga ttgcaactct
     4021 tgtgggtaag atgtgtaaac ccgcctgcgc cgatcagcgc agctgacttc acctttcctg
     4081 ggctctgaac ttatttcgtt gcggtatcac tgacctcttc caagtccttt tcaagtt
//
LOCUS       DATLJT010001051         4495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2830817,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001051 DATLJT010000000
VERSION     DATLJT010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830817"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..2129
                     /locus_tag="VM910_24140"
     CDS             366..2129
                     /locus_tag="VM910_24140"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HVI65645.1"
                     /translation="MARLLSVNVGLPRDIEWKGRTVHTGIWKTPVRGRCRVSRLNLDG
                     DGQGDLAGHGGEQRAVFVYQIESYRYWQEQLKRADFVHGQFGENFTIEGWPDDAVCIG
                     DRYQIGSALFEVTQPRVTCYRVGIRMNEPRMPALLTSSGRPGFYFRVLQEGEVGAGDE
                     IVKVGETKERMSVAEINALLYSPDHARDRLQRALRIEALSPGWRSSFEALLQSQPTAA
                     GSGNAGLAPAAAAHPVAPGFQPLAVAAIDQESADVLSLTMQSPDGQPLPPALPGQYVV
                     LRLRRTGGGSPLFRSYSVSGPLSTQRYRISVKVEPNGAAGTYLREHVRLGDALDVSSP
                     RGSFILQSGERPVVLLSAGIGATPVLAMLHALAAARSTRQVLWLHGARDQQHHPFAAE
                     VRHLVRALSHGRSYVCYSRPGARDRTNEDFDATGRLSRSILEEVGLPRDADVYVCGPP
                     RFMAEMKDALATLGVAPQRIHVELFNGSESMTPGVIGAAARAPHPPKDEVTTGPLVSF
                     ARSGIAAHWKAAAYQSILELAEACDVPVRWSCRTGVCHNCESGLVSGAVVYEPEPLEK
                     PADGNLLVCCSQPIRDIVIDL"
     gene            2151..4091
                     /locus_tag="VM910_24145"
     CDS             2151..4091
                     /locus_tag="VM910_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVI65646.1"
                     /translation="MTTFPRSVRVIEHTLIPLKDGTALAARIWLPDDAEQNPVPAILE
                     YLPYRKRDGTYERDALTHPYLAGHGYAGVRVDIRGCGESDGLLFDEYAKQEQDDGVEV
                     IAWLAAQPWCSGVVGMMGISWGGFNGLQIAARRPPALKAIVTICSTDDRYADDVHYMG
                     GTLLAADGLEWGSFFFGSMCLPPDPMLVGDRWRAVWLERLANVPLFFELWLQHRRRDG
                     YWKHGSVCEDYGAIQCPVYAVGGWTDAYTNAIPRLLERLSVPRKGLIGPWAHGYPHFA
                     LPGPQIGFLQEMLRWWDHWLKGVDTGVMDEPMLRAWMTDSVKPASHHETLPGRWVAER
                     SWPSPGITPRRLFLTDEGLRNEAAPLTARTLCSPQTVGKCSGDWVPFGRGHDQAYDQR
                     DDDARSLIFETPPLDAPIETLGAAIVTLDVVSDRPIAKLVVRLCDVHPSGESLRVSYG
                     ILNLTHRDGHDKPAPLAVGQRYRVRIQLNDAGSVFPAGHRIRLALSTAYWPMIWPSPE
                     LATLLISGGTLDLPVREPQATDALLPPLPGPASAPPEKPTVTRRGDLHIERIDRLGLE
                     LGTENKSQFHVEEDDPLSAVADLRRTWTMSRDAWQIRVETQMRLSCTRDVFLLRGSLR
                     AWDGANEVCRREWDRAIPRDFL"
     gene            complement(4234..4413)
                     /locus_tag="VM910_24150"
     CDS             complement(4234..4413)
                     /locus_tag="VM910_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65647.1"
                     /translation="MCWEVDYKFFAEQQKKAQDARIKQEQRAGVIDRLLNDANKQDEK
                     TEVEGAPAKEAAPAK"
BASE COUNT          786 a         1386 c         1453 g          870 t
ORIGIN      
        1 gcggctagtg tcccgattcc gaagttcgca agtccttgcg gcacactcgc gtgcgaactt
       61 cggaatcaaa gggacactag caaacttatg actctagtgt ggtttttgga tttgaagttc
      121 ctatgagagc tcgctggata ctcgtaggaa cttcaaatcc accacactag tgtcccgatt
      181 ccgaagttcg caagtccttg cggcacactc gcgtgcgaac ttcggaatca aagggacact
      241 agcaaactta tgactctagt gtggtttttg gatttgaagc tcctatgaga gctcgctgga
      301 tactcgtagg aacttcaaat ccaccacact agggagggct tccggtcccc accaaaggga
      361 gcgacatggc tcgactacta tcggtgaacg tcggacttcc gcgcgacatc gagtggaagg
      421 gtcgcactgt acacaccggg atctggaaga ctccggtgcg tggccgctgt cgagtcagcc
      481 ggttgaatct ggacggagat ggtcaaggcg acctggccgg ccacgggggt gagcagcgcg
      541 cggtcttcgt ctaccagatc gaatcgtatc gctactggca ggagcagctg aagagggccg
      601 acttcgtcca cggacagttc ggcgagaatt tcacgatcga aggatggccc gacgatgccg
      661 tttgcatcgg cgaccgctat cagatcggca gcgcactgtt cgaggtcact cagcctcgcg
      721 ttacctgtta tcgcgttggc attcggatga acgagccccg aatgccggca ttgctgacat
      781 ccagcggacg cccggggttc tacttccgcg tcctgcagga aggcgaggtg ggcgccggcg
      841 acgaaatcgt gaaggtggga gagacgaagg agcgaatgtc cgtcgcagag atcaacgcgc
      901 tcctctattc gcccgatcat gcgcgcgatc gactgcagcg cgccttgcgg atcgaagcac
      961 tttcgcccgg atggcgctcg tcgtttgagg cattactgca gagccaaccg actgccgcgg
     1021 ggagtggcaa cgcggggctc gcgccggcag cagccgcgca tccggttgca ccaggatttc
     1081 agccgctcgc ggtggcggcg atcgatcagg agtccgcgga cgtcctctcg ctgacgatgc
     1141 agagcccgga cggtcagccg ctgccaccgg ctctgcccgg ccagtacgtc gtcttgcgtc
     1201 ttcgacggac cggcggcggc tcgccactct ttcgcagcta ctcggtctcg ggtcctctct
     1261 ccacgcagcg ctatcggatt agcgtgaagg tcgagccgaa tggggcggct ggaacctacc
     1321 tgcgggagca tgtccgcctg ggcgatgctc tcgacgtcag ctcgccgcgc ggaagcttca
     1381 ttctgcagtc gggagagcgg cccgtggtgc tgctcagcgc gggaatcggg gcgacgcctg
     1441 ttctggcgat gctgcacgcg ctggcagcgg ctcgatcgac acggcaggtc ttgtggctgc
     1501 atggagctcg cgatcaacag catcatccat tcgccgccga agttcgccac ctcgtgcgcg
     1561 cgctctcgca cggccgcagc tatgtttgtt acagcaggcc cggcgcgcgc gacaggacga
     1621 atgaggattt cgatgcgacc ggtcgcctgt cccgatcgat cctcgaggag gtcggcctcc
     1681 cacgcgacgc ggatgtctac gtctgtgggc cgcctcgctt catggccgaa atgaaagatg
     1741 cgctcgcaac cttgggcgtt gcgccgcagc ggattcacgt cgaactcttc aacggcagcg
     1801 aatcgatgac tcctggtgtc atcggcgcag cggcgcgagc tccgcatccg cccaaggacg
     1861 aggtcaccac cggtccgctg gtatcgttcg cgcgcagtgg catcgccgca cactggaagg
     1921 cggcagccta ccagagcatt ttggaactgg ccgaggcatg cgacgttccg gtccgttggt
     1981 cgtgccggac cggcgtttgt cacaactgtg agagcggttt ggtttcgggg gcggtcgtct
     2041 acgaaccgga gccactcgaa aagcccgccg acggcaacct tctcgtatgc tgctcacaac
     2101 cgattcgcga catcgttatc gatttgtgaa acgggggaat ccctgtgatc gtgacgacgt
     2161 ttccccgcag cgtgcgcgtc atcgagcaca cgttgatacc gttgaaagac ggcacggcgc
     2221 tcgcggcgcg catctggttg ccggacgatg cggaacagaa cccggttccg gccatcctgg
     2281 agtatctccc ctaccgcaag cgcgacggca cctacgaacg ggatgcgctg acacatccgt
     2341 atctggccgg ccacggctac gccggcgttc gcgtcgacat tcgaggttgt ggcgaatcgg
     2401 atggcttgct gttcgacgag tacgcaaagc aagagcagga cgatggcgtc gaggtgatcg
     2461 cgtggctggc cgcgcagccc tggtgcagcg gcgtggtcgg gatgatggga atctcctggg
     2521 gcggttttaa tggactgcag attgcggcgc gccgcccgcc cgccctcaag gccatcgtca
     2581 caatctgctc gaccgatgac cgctatgcgg acgatgtgca ttacatgggc ggcacgcttc
     2641 ttgcggccga cgggctcgag tggggctcgt tctttttcgg ctcgatgtgc ctgccgcccg
     2701 acccgatgct ggtcggcgat cgctggcggg ccgtgtggtt ggagcgcctg gcgaacgtgc
     2761 cgctgttctt cgaactttgg ttgcagcacc ggcgacgtga tgggtattgg aagcatggat
     2821 cggtgtgcga ggattacggg gccatccaat gccccgtgta cgccgtcggc ggctggaccg
     2881 atgcctatac caatgcaatt ccacgcctgc tcgagcggct gagcgtaccg cgcaagggtc
     2941 tcatcgggcc gtgggcccac ggttatccgc attttgcgct gcccggcccg cagatcggct
     3001 tcctgcaaga gatgctgcgg tggtgggatc attggctcaa gggcgtcgat accggcgtga
     3061 tggatgagcc catgctgcgc gcttggatga cggacagcgt gaaacctgca tcccatcacg
     3121 agaccttgcc ggggcgatgg gttgccgaac ggtcgtggcc atcgccggga ataacaccgc
     3181 ggcggctgtt cttgaccgat gaggggctgc gaaacgaagc tgcccctttg acggcgcgca
     3241 cgctgtgctc gccgcagacc gtcggcaaat gttcgggcga ctgggttccg ttcggtcgcg
     3301 gacacgatca ggcctatgat caacgggacg acgatgcccg ctcgctcatc ttcgagacgc
     3361 cgccactcga tgcgccgatc gagaccctcg gcgcggcgat cgtcacgctc gacgtcgtgt
     3421 cggacaggcc gattgcgaag ctggtggttc ggttatgcga tgtgcatccg tccggcgagt
     3481 cgctccgtgt cagctacggc atactcaacc tcacccatcg cgacggacac gacaagcccg
     3541 cgccgctggc ggtgggccag cgctaccgcg tgcgcatcca gctcaacgat gccggctcgg
     3601 tgttcccggc cgggcacagg ataaggctcg cgctatccac ggcgtactgg ccgatgatct
     3661 ggccttctcc cgagctggcg acgctgctga tttcgggcgg cacgctcgat ctgccggtac
     3721 gggagcccca ggccacggac gcgctgctgc caccgctgcc cgggccggcg tcggcaccgc
     3781 ccgagaagcc gaccgtgacc cgccgcggcg atctgcatat cgagcgcatc gaccgcctcg
     3841 ggctggaatt gggtacagag aataaatccc agtttcacgt cgaggaggac gacccgctca
     3901 gcgcggtggc cgatctgcga cggacgtgga cgatgtcgcg cgacgcatgg caaatccgcg
     3961 tcgagacgca gatgcggctc tcatgcacgc gcgacgtctt cctgctgcgg ggaagtttac
     4021 gcgcgtggga cggcgcgaac gaagtctgcc gtcgcgaatg ggaccgcgct attccccgag
     4081 acttcctatg acttgagccg cgctatgact gagccgcgcg catcgctcat tcgtcgatac
     4141 gatagctctc cgatcaaggc gacgagcgtg ccgcgatcgg caaaccagcg atgacgatca
     4201 gccccgccgg agcgagggcc gtgggcgcat cggctatttt gcgggtgcgg cctctttggc
     4261 cggcgctcct tcgacctcgg tcttctcgtc ttgcttattg gcgtcgttca ggagccgatc
     4321 gatgacgccc gcgcgctgct cctgcttgat ccgagcgtcc tgcgccttct tctgttgttc
     4381 agcgaagaat ttgtagtcga cttcccaaca cattggatcc tccatctgtg acggaaccaa
     4441 cggcataaca acgctcatat catagcatat ggttccgagg tgtggccagc gcggg
//
LOCUS       DATLJT010001052         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_73035,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001052 DATLJT010000000
VERSION     DATLJT010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73035"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VM910_24155"
     CDS             <1..1435
                     /locus_tag="VM910_24155"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65648.1"
                     /translation="AARAQQQAMVGEPTPSQQSLLALGWLNFFLSGVQASFGPICAAY
                     LAAQGWTAQNIGFVLSIGGTASLASQVPGGLLLDAVRAKRLLIAAGAVIVAFSIAIFS
                     LWPSFPVVALAEVLQGITGGVLGPAVVAITLGLVGHAALAGRLGQNQRFAAAGGVAVT
                     LTMALLAYSEPLWAMFVPVVLAVPMFVALNQIRAQEIDFGRASGAKHAYADRPQGAGR
                     DGTLLKNRRLLIFAACAVLFQVANASMLPLASGLLAYEGMRQAAPLVAALIIVPQLIE
                     VLLAPWVGQRAEKSGRKPLLLVAFAALPIRAMLFALTSNPLALIVTQVLDGISGVVLG
                     VMTALVIADVTKGTGRFNLAQGMFGTVMGIGASLSPTLSGLIAHHFGHSVGFMSLAGE
                     GFVALVVLAVFLPETKEETPLSPGVTEGSPLNQISTVAPRWQFTSAMRRALLVQDAVA
                     TSAAIYGCAPRGKLSNRQRGALSSSPS"
     gene            2169..2375
                     /locus_tag="VM910_24160"
     CDS             2169..2375
                     /locus_tag="VM910_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65649.1"
                     /translation="MEIYEVELCQHGRWEQQHARFVAARDADEAAYKVTGEHLRSEGE
                     RRKLRLRVKRVGNGSPPPKLFYAA"
BASE COUNT          461 a          808 c          819 g          559 t
ORIGIN      
        1 cgcggctcgc gcgcagcaac aggccatggt gggggagccc acaccctcgc agcaaagcct
       61 gcttgccttg ggctggctca acttctttct gtccggtgtg caagcctcct ttggacccat
      121 ttgtgccgcc tatctagcgg cccaggggtg gactgcccag aacatcggat ttgtcctcag
      181 cataggcggc accgccagtt tggctagtca ggtgcctggc ggactgcttc tggatgccgt
      241 gcgagcgaag cgactgctga tcgcggccgg tgcggtgatc gttgccttca gcatcgcgat
      301 cttcagtctt tggcccagct tcccggtggt cgccctcgcg gaagtgttgc aggggatcac
      361 aggcggtgtc cttgggccgg cagttgttgc catcaccctc gggctggtcg gccacgccgc
      421 gttggcagga cggcttggac agaaccagcg cttcgcggcc gccgggggcg tcgcggttac
      481 cctgacgatg gccctcctcg catattccga gccgctctgg gcaatgttcg ttcccgtggt
      541 actcgccgtt ccgatgttcg tcgcgttgaa ccagattcgc gcccaagaga tcgattttgg
      601 gcgtgcctct ggcgctaaac acgcctatgc cgatcgtccc cagggagccg gacgcgacgg
      661 gactcttttg aagaaccgcc ggctattgat ctttgccgcc tgcgcggtcc tttttcaggt
      721 cgccaatgca tccatgctgc cgctcgcgag tgggctgctg gcgtatgagg gcatgcgtca
      781 ggcggcaccg cttgtagcgg ccttgattat agtgccgcag cttatcgagg tgctgctcgc
      841 gccttgggtc ggacagcgag ctgaaaaaag tggtcgtaag ccactgctgc tcgtcgcttt
      901 cgccgcccta ccgatccgcg cgatgctgtt tgctttgacc agtaatccgc ttgccttgat
      961 agtaacccag gtgctggacg gtatcagtgg ggttgtactc ggggtgatga cggcactggt
     1021 catcgcagat gtgacgaaag gcacgggacg cttcaatctc gctcagggca tgttcggcac
     1081 ggtaatgggt atcggtgcct ccctcagtcc gacactttcg ggcttgatcg cccatcattt
     1141 tggacattcg gtggggttta tgagtctcgc aggcgagggg ttcgtcgcac tcgtggttct
     1201 tgcggtcttt ctgccagaga cgaaggagga gactcccctt tctcccggag tgacagaggg
     1261 ctcgccgtta aatcagatct ccaccgttgc tcctcgttgg cagttcacgt cggcgatgcg
     1321 ccgtgctctc cttgtgcaag acgcggtcgc tacctccgct gccatatacg gttgcgcgcc
     1381 ccgaggaaag ctctcgaacc gtcagcgtgg cgccctttct tcgagtccct cataagcaaa
     1441 cgacaccgca tagctacccg tcatctgctg gcggtgctgc ggaagcatgc gcctgcatcg
     1501 catcacgcac cttcgccccc accatgaacg gcgccgaata agcgcggtag gcgttgcgca
     1561 gaggcgcatc atagaggctg cgaaacgcgg cgtggctcag cgcgggcgag cctcaaactg
     1621 ctttacgaca ggaattacgc acccgtgctg gatcacgctc gccgtacttg gccagctcga
     1681 ttatgcgctt ggcgatcata acaggggtca gtgcgatcaa ctaggcgcaa ggcccgcaag
     1741 gtatcctcga atgcagaaga gatcgcgatc ccaatatcgg cccgataggc acgatgtgcc
     1801 ccgcagcccg caaagccgca cgtcagcctt gcacaaatgg ctcgcggggg ctgcctgatg
     1861 gccgactcaa cccacgccaa atgcgggtga ttgcagaggc gctgacggcg ctgcgcagac
     1921 gccgctggca ttgcgcgtcc atgtcgccgc tgccgctggg cttcgttgcg ccgttcttgc
     1981 cctacgggcc ccccgcagcc gccttccggc gccctctggc tgcatgaaat caagcacgat
     2041 ggctcggcag tgacctgacc aaccgctcga cctctcgaca gagcgtccag agcgcgggtc
     2101 cgcgccagat tcttgtcaca aaatccaatt tcaaatgcgg ggataagatc gggtctcggg
     2161 agatcaggat ggagatatac gaggtcgagt tatgccagca tggccgctgg gagcagcaac
     2221 atgcccggtt tgtcgcggcg agagacgccg atgaagcggc ctacaaagtg acgggagagc
     2281 atcttcgcag cgaaggggaa cgcaggaagc tacggctgcg cgtgaagagg gtcggaaacg
     2341 gcagtccccc accaaagctg ttttacgccg cttgatccgc agcgttgggt tgccgatgac
     2401 tgtcgtcgcg cggatcgagc gcggccgaca tctccggcca cagctgacgc gatctgctgg
     2461 tctactgcgg ctcgggccga tgcgatcata agcggcgatt ggctgtccga taatgtgccg
     2521 gtgcggtccg acggtttctt caacccgact ggtgagcttc aatgtggtgg atcaggtcgc
     2581 ctacgaactc tatctcttcg ctggcgtccg caatctctcg atttacgaat tctttctcga
     2641 tcaagat
//
LOCUS       DATLJT010001053         5325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2140513,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001053 DATLJT010000000
VERSION     DATLJT010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140513"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VM910_24165"
     CDS             <1..972
                     /locus_tag="VM910_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HVI65650.1"
                     /translation="APEVPALRTETNPGRPLRLNTGRIRDQWHTMTRTGLSPRLGAHL
                     PEPFVEVHPDDANRFGIVDDSFARVTTDYGQCVLKVVVSDRQQRGMLFAPIHWSQSNA
                     SSARVGALVAPFTDPYSGQPEAKSTPASIQPYEYVFRGFMLSRKEIHLPSSVWWARAA
                     VAGGVGYLFADNADLSRWAAWLRAVAGTDLAEYCDFGGGVYRAASFASDRIEACLFVG
                     PAHDAGDWDVVKSLFAADSLGDDQRAMLLSGRSSEGLVSAGPIVCACFGVGRATICDA
                     IAGGALTAAEIGAKLKAGTNCGSCLPELKQLIAQTPVKALPQVSAAH"
     gene            1117..2019
                     /locus_tag="VM910_24170"
     CDS             1117..2019
                     /locus_tag="VM910_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI65651.1"
                     /translation="MLYLETPPKLIETRVFSAMPENFRRKGVRTEWADANRPGHPADC
                     FIEGPSFDAAGNLYIVDIPFGRIFTIAPDGSWSLAIEYEGWPNGLKISPEGRILVADY
                     MHGIMELDTKAGRMRKVLTSRSSESFRGCNDLHLARNGDIYFTDQGQTGLHDPTGRVY
                     RLATNGRLDCLIDTGPSPNGLVLDPNEAVLFVAMTRDNAVWRLPFMRDGSVSKVGRFC
                     SLFGPSGPDGMTMDRAGRLFVAHASLGHVFVFAPNGELIARIKSCVGPTCTNVAIGGP
                     SSDRLYITESATGSVLVADISELS"
     gene            2041..2880
                     /locus_tag="VM910_24175"
     CDS             2041..2880
                     /locus_tag="VM910_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI65652.1"
                     /translation="MKTKPFGPTGQNVSVIGQGTWYIDRGDRKAAISALRRGVELGMT
                     HIDTAEMYGDAELVIAEAIAGRRDSVFLVSKVLPSNASRKGTIVACERSLKRLKTDRL
                     DCYLLHWRGSYPLADTVAAFEELVRAGKIRSWGVSNFDADDLDEMLDVAGKGRIACNQ
                     VLYHLKERAIEHAVLPWCERHGVAVVAYSPFGHNDFPSPRTRAGEVLQKIASAHHATP
                     RQVALGFLTRRNQVFAIPKTGSAEHATENAAAGDLALSDDEIAALDAAFPRGPKPRSL
                     PMI"
     gene            3102..4097
                     /locus_tag="VM910_24180"
     CDS             3102..4097
                     /locus_tag="VM910_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI65653.1"
                     /translation="MTPPPAAAAVARLDSPVLSLPEKKTAARRAPARADRPFKGIALI
                     LASTVFLGVSDVTAKYLSATLPSIEIAWLRFLVFALIMVPAMVPGTPIYSLKTRSLGL
                     QLMRGAALLGSSLFFISGLRYLPIAEASATGFVSPLFVTALSIIFLSERVGVRRWLAT
                     ACGLIGVLIILRPGTSAFHPAAFFPIVSALAWASTLIMTRMMSGREHAVTTMTYSSIA
                     GVAILSGLVPFVWVTPSWHDVMFGILIGVASTAGQWIVVLAFRYADASVLAPFSYAQL
                     LWVSILGFLVFGEVPDAWTIVGAAVIVGSGLYTAHRERVRRSQLLALEAEPSPNA"
     gene            4189..>5325
                     /locus_tag="VM910_24185"
     CDS             4189..>5325
                     /locus_tag="VM910_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVI65654.1"
                     /translation="MITLSSKDVLPEDGTRGTLVGRIFLPGVGGPAVAAIRDSGVFDV
                     SARFPTVSALAEENDAAAALRATKGERIGDLDALVANTPPDLRDPNKPWLLAPVDLQV
                     LKAAGVTFAISMLERVIEERARGNPASAEAIRKEVVRLVGDDFSKLRPGSAEAMRLKQ
                     VLIEQNAWSQYLEVGIGPDAEVFTKAPVMSAVGTGMDAGLHPKSTWNNPEPEVVLVVS
                     SRGSIVGATLGNDVNLRDFEGRSALLLSKAKDNNASCAVGPLLRLFDDRFSLDDVREM
                     DVGLTVTGPDGFVLEGHSSISKISRDPTDLVAQTIGAVHQYPDGFALFLGTMFAPVKD
                     RDAPGQGFTHKRDDIVTIASPKLGKLTNRMRASDECEPWTFGVGA"
BASE COUNT          871 a         1724 c         1690 g         1040 t
ORIGIN      
        1 gcgccggaag ttccagcgct gcggaccgag accaatcccg gccgcccatt gcgactcaac
       61 accgggcgca ttcgcgacca gtggcacacc atgacccgca ccggcctcag tccaaggctg
      121 ggcgcgcatc tgccggagcc gttcgtcgaa gtgcatcccg acgatgcgaa caggttcggc
      181 atcgtcgacg acagttttgc gcgcgtcacc accgactacg gccaatgcgt cctcaaggtc
      241 gtggtgagcg atcggcagca gcgcggcatg ctgtttgcgc cgatccattg gagccagagc
      301 aacgcctcca gcgcgcgcgt cggagcgctg gttgcgccct tcaccgatcc ctattccggc
      361 cagcccgagg ccaagtcgac gccggcatcg atccaacctt acgaatatgt gttccgcggc
      421 ttcatgctgt cgcgcaagga aatccatttg ccgtcgagcg tctggtgggc gcgtgctgcg
      481 gtcgcgggcg gcgtcggcta tctgttcgcc gacaatgcgg atctgtcgcg ctgggcggcg
      541 tggctgcgcg cggttgccgg caccgatctc gccgagtact gcgatttcgg cggcggtgtc
      601 tatcgcgcgg cgtcctttgc gtcggatcgc atcgaggcgt gtctgttcgt cggtcccgcg
      661 catgacgcgg gcgactggga cgtggtgaag agcctgtttg cggcggacag tcttggcgac
      721 gatcagcgcg cgatgctgtt gtccggccgc tccagcgaag gccttgtcag tgcgggcccg
      781 atcgtctgcg cctgttttgg cgtcggccgc gccactatct gcgacgcaat cgcgggcggc
      841 gcgctaaccg ccgccgaaat cggcgcaaag ctcaaggccg gcaccaattg cggatcctgc
      901 ctcccggaat tgaagcaact gatcgcgcag acgccggtga aagcgttgcc gcaggtatcg
      961 gcggcgcatt agcttctcgt gagcttctct cggcgtcatt ccgggtctgc gcttcgcgca
     1021 tcgcggaacg acggcaccgg acatcaagcc caatcgacgg cacgcagcct gtcctctatg
     1081 gtgcgcctgc tccgaacgaa caggcgagcc gagaaaatgc tgtatctgga aacgccgccg
     1141 aaactgatcg aaacccgcgt gttctccgcg atgccggaaa attttcggcg caagggtgtg
     1201 cgcaccgaat gggccgatgc caaccggccg ggccatcccg ccgattgctt catcgaagga
     1261 ccatcgttcg acgcggcagg caatctttac atcgtcgaca tccccttcgg ccgcatcttc
     1321 accatcgcgc ccgatggatc atggtcgctt gcgatcgaat atgagggttg gccgaacgga
     1381 ctgaagatca gcccggaggg gcgcattctg gtcgccgatt acatgcacgg catcatggag
     1441 ctcgacacca aagcagggcg gatgcggaag gtgctgacct cgcgcagctc cgaatccttc
     1501 cgaggttgca acgatcttca tcttgccaga aatggcgaca tctatttcac cgatcagggt
     1561 cagaccggcc tgcacgatcc cacgggccga gtctatcggc tggcgacgaa cggccggctc
     1621 gactgcctga tcgacaccgg gccgagtccc aacgggcttg tgctcgaccc gaacgaggcc
     1681 gtgctgtttg ttgcgatgac gcgcgacaat gcggtgtggc gcctgccgtt catgagggac
     1741 ggcagcgtgt cgaaggtcgg tcgcttctgt tcgctgttcg ggccgagcgg acccgacggc
     1801 atgaccatgg atcgcgcggg tcggctgttc gtggcgcacg cgtcgcttgg tcatgtcttc
     1861 gtgtttgccc cgaacggcga attgatcgcg cggatcaagt cctgcgtcgg gccgacctgc
     1921 accaatgtcg cgatcggcgg gccgagcagc gaccgccttt acatcacgga atccgcgacc
     1981 ggcagcgtgc tggtcgccga tatcagtgaa ctctcgtagc agtcgcgcgt tggtttccac
     2041 atgaagacaa aaccctttgg ccccactggc cagaacgtgt ccgtgatagg gcagggcacc
     2101 tggtacatcg atcgcggcga ccgcaaagcg gctatttccg cgctccgccg cggcgtcgag
     2161 ctcggcatga cccatatcga caccgctgag atgtatggcg atgcggaact cgtgattgcc
     2221 gaggcgatcg caggccggcg cgactctgtg ttcctcgttt ccaaggtgtt gccgagcaac
     2281 gcctcgcgca aaggtacaat cgtcgcctgc gagcgctcgc tgaagcgact caagaccgat
     2341 cgtctcgatt gctatctgtt gcactggcgc ggttcatatc cgctcgccga cacggtcgcg
     2401 gccttcgagg aacttgtgcg cgcgggaaaa atccgttcct ggggcgtcag caatttcgat
     2461 gccgacgatc tcgacgaaat gctggatgtc gcgggcaagg gccggatcgc ctgcaaccag
     2521 gtgctctatc atttgaagga gcgcgccatc gagcacgcgg tgctcccgtg gtgcgaacgt
     2581 cacggcgtcg ccgtggtcgc ctattcgccg ttcggccaca atgatttccc gtcgcctcgt
     2641 accagggccg gcgaagtgtt gcagaagatc gccagtgccc atcatgcaac cccgcgccag
     2701 gttgcgcttg gctttctcac ccgccgaaat caggtattcg cgattcccaa aacaggtagc
     2761 gccgagcatg cgaccgagaa tgccgcggcc ggcgatctcg ccttgagcga tgacgagatc
     2821 gcggcgctcg atgcggcctt tccgcgcggg ccgaagccgc gcagcctgcc gatgatctag
     2881 gtacactctt ggctctccgc gcgcggggaa ggagttcact gtcatcgcag cgccggctcg
     2941 aatttatctc agcgtggttc ggcaagcaca cttcttaaca aaatgtttca gcgctgcccg
     3001 catcgcgcat atcggcatcc cgttatcggg tgttgtcgtg caacgagatt tcgcgttttt
     3061 tagagaccta cgcgattcga aattttgcca gagtttttac tgtgaccccg ccacccgccg
     3121 cggcagccgt cgcccggctt gacagccccg tattgtcgtt gcccgagaag aagaccgccg
     3181 cacgtcgcgc cccggcgcgc gccgaccggc cgttcaaagg catcgcgctg atcctggcgt
     3241 ccaccgtatt cctcggcgtc tccgacgtta ccgcaaaata tctgtcggcg actttgccgt
     3301 cgatcgagat tgcctggctc cgctttctgg tgtttgcgtt gatcatggtg ccggcgatgg
     3361 tgccgggcac gccgatctat tcgctgaaga cgcggagcct cgggctgcaa ttgatgcggg
     3421 gcgccgcgct gctcggctcg tctctcttct tcatctcggg cctgcgttat ctgccgatcg
     3481 cggaggcctc cgccaccggc ttcgtgtcgc cgctgttcgt caccgcgctg tccatcatct
     3541 ttctgtccga gcgtgtcggc gtgcgccgct ggcttgcgac cgcatgtggc ctcatcggcg
     3601 tcctgatcat cctgcgtccc ggcaccagcg cgttccaccc ggctgccttc tttccgatcg
     3661 tctcggcgct cgcctgggcg tccacgctga tcatgacgcg catgatgagc gggcgagagc
     3721 acgccgtcac caccatgacc tattcctcga tcgcgggcgt tgccatcctc tccggcctcg
     3781 ttccgttcgt ctgggtgacg ccgagctggc acgatgtcat gttcggcatt ttgatcggcg
     3841 ttgcctcgac cgcgggacag tggatcgtgg tgctggcctt ccgctatgcc gacgcgagcg
     3901 tgctcgcgcc cttctcctac gcgcaactgc tgtgggtctc gatcctcggt ttcctggtgt
     3961 tcggcgaagt gcccgatgcc tggaccatcg tcggcgccgc cgtcatcgtc ggcagcggtc
     4021 tctacaccgc gcatcgcgag cgggtgcggc gctcgcaact cctggcgctg gaagccgagc
     4081 ccagcccgaa cgcctgaaac cacggccctg cttccctccc gcgcaattgt tgcccgctac
     4141 acctgtcggg ccgctgtgct atgaagcagg acgcacaggg gaagcgccat gatcacactt
     4201 tcaagcaagg acgtcctgcc ggaagacggc acgcgcggca ctctcgtcgg gcggattttt
     4261 ctgcctggcg tcggaggccc ggctgtggct gcgattcgcg atagcggcgt gttcgacgtg
     4321 agcgcgcgct ttccgaccgt gagcgcgctt gccgaggaga atgatgcggc ggctgcgctg
     4381 cgcgcgacca aaggcgagcg catcggcgat ctcgatgcgc tggtggcgaa cacgccgccc
     4441 gacctccgcg atcccaacaa accctggctg ctcgcgccgg tggacctgca ggtgctgaaa
     4501 gccgccggcg tcaccttcgc catctcgatg ctggagcggg tgatcgagga gcgggcgcgg
     4561 ggcaatccgg cctccgccga ggcgattcgc aaggaagtgg tgcggctggt cggcgacgat
     4621 ttctcaaaac tcaggccggg ctcggcggaa gcgatgcggc tgaagcaggt gctgatcgag
     4681 cagaatgcct ggagccagta tctcgaagtc ggcatcggcc cggatgccga ggtcttcacc
     4741 aaggcgccgg tcatgtccgc ggtcggtacc ggcatggatg ccggcttgca tccgaaatcg
     4801 acctggaaca atccggagcc ggaggtcgtg ctcgtcgtat ccagccgcgg cagcatcgtc
     4861 ggcgcgacac tcggcaatga cgtcaacctg cgcgatttcg agggacgctc ggcgctgctg
     4921 ttgtccaagg ccaaggacaa caatgcctcc tgcgccgtcg gcccgctctt gcgtctgttc
     4981 gacgatcgct tctcgcttga tgacgtgcgc gagatggatg tcgggttgac cgtgaccggg
     5041 ccggacggtt ttgtgctcga ggggcattcc tcgatcagca agatcagccg cgatccgacc
     5101 gacctcgtcg cacagaccat cggtgcggtg catcaatatc cggacggatt tgcgctgttt
     5161 ctcgggacga tgttcgcgcc ggtcaaggac cgcgatgcgc cgggtcaggg gttcacgcac
     5221 aagcgcgacg atatcgtgac gatcgcatct cccaagctcg gcaagctcac caaccgcatg
     5281 cgcgcgagcg acgagtgcga gccctggacg ttcggggtcg gcgcc
//
LOCUS       DATLJT010001054         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_533509,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001054 DATLJT010000000
VERSION     DATLJT010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533509"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VM910_24190"
     CDS             <1..522
                     /locus_tag="VM910_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65655.1"
                     /translation="GPLASDQIPGASRVPQSFSHRLMAQDPIRTKSGTVRIADSKNFP
                     ASVTIAAALVEILPGGMRELHWHPNTDEWQYYIEGQGRMGVFASSGDARTFDYQAGDV
                     GFVPFAMGHYIENTGTTPLRFLEMFKSSYYADVSLNRWMALTPPELVQGTLKLDQTVM
                     SALCKGPAPVVPA"
     gene            complement(558..1649)
                     /locus_tag="VM910_24195"
     CDS             complement(558..1649)
                     /locus_tag="VM910_24195"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVI65656.1"
                     /translation="MLRRHPTSFPATQQTAARRPDMATASATQVTPERIMQFTFGFAP
                     PLIIEAAMRHRVFDALDEGAKTIETLCAETQTSLRGLRALLNALVGLELLAKDDGGRY
                     ALTPESAAFLVRGKPAFHGAFFLLTSERMLSEWAKLHDIVRSGRPEKRINREQDGVPF
                     FRQFVEDIFPIHYAAAQQLSEALEVSKAAAPLSVLDLAAGSGVWSVALALRSPHVRVT
                     AVDWPGVIPITQKVTARFGVADRFRFVAGDLLEANFGSGHAIATAGHILHSEGEARSR
                     VLLEKTFDALAPGGTLAIAEILVDANRTAPLPALIFAVNMLVNSDQGDTFSLEEIGGW
                     LRDAGFAHVRTVDAPGLAPLLILATKPGH"
     gene            complement(1814..2203)
                     /locus_tag="VM910_24200"
     CDS             complement(1814..2203)
                     /locus_tag="VM910_24200"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65657.1"
                     /translation="MSATDTGAAFPYATYLDIKFDPLKLIDVPSLVQACTDQWYNQTL
                     CKVNDSVVRLGVMQGEYHWHKHDDDDEFFFVLSGRFIIDLEGRSVTLSPQQGFVVPKR
                     VVHRTRAPEKAVILMIETAAIVPTGDA"
     gene            complement(2280..>2496)
                     /locus_tag="VM910_24205"
     CDS             complement(2280..>2496)
                     /locus_tag="VM910_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65658.1"
                     /translation="GVVEALYETLDRLHKEGLTLLLAEQSIPLALDIADYAYVLQTGR
                     TVLEGKAVDLEKNEQVQEIYLGIGTRT"
BASE COUNT          471 a          771 c          779 g          475 t
ORIGIN      
        1 gggccgcttg cgtccgacca gattcccgga gcatcgcggg tgccgcagtc gttcagtcat
       61 cgcctgatgg ctcaggaccc gattcggacc aagagcggca cggtccgcat cgccgattcc
      121 aagaatttcc ccgcctccgt cacgatcgcg gccgccctgg tcgagatcct gccgggcggg
      181 atgcgcgagc tgcattggca tccgaacacc gacgaatggc aatattacat cgagggacag
      241 gggcgcatgg gtgtgttcgc gtcgtccggc gatgcgcgca ccttcgacta tcaggccggc
      301 gacgtcggct tcgttccgtt tgccatgggc cattacatcg agaataccgg caccacgccg
      361 ctgcggtttc tcgagatgtt caagagcagc tactacgccg atgtctcgct caaccgctgg
      421 atggcgctga ccccgcccga gctggtgcag ggaaccctca agctcgacca gacggtcatg
      481 agcgcgttgt gcaaagggcc tgctccggtc gtgccggctt gagccgggcg cgggctgccg
      541 atccctgcgg cgaaggttca gtggcccggc ttcgtcgcca agatgagtaa gggcgctagc
      601 ccgggagcgt ccacggtgcg cacgtgcgcg aagccggcgt cgcgcagcca accgccgatc
      661 tcttccaagg agaaggtatc gccttggtcc gagttcacca gcatgttgac ggcaaaaatc
      721 aacgccggca gtggcgcggt gcggttggcg tcgaccaaga tctccgcgat cgccagcgtc
      781 cctcccggcg cgagcgcatc gaaggtcttt tccagcagca cgcggctgcg cgcttcaccc
      841 tcgctgtgca ggatatgccc tgcggtggcg atggcgtgtc cgctgccgaa attcgcttcc
      901 agcaagtcac ccgctacgaa acggaatcgg tccgcgacgc caaagcgcgc cgttaccttc
      961 tgcgtgatcg ggatcacgcc cggccaatcc accgccgtca cgcgcacgtg cggcgatcgc
     1021 agcgccagcg ccacgctcca cacgccggat ccggcggcca aatcgagcac ggacaacggc
     1081 gcagcggctt tcgacacctc tagcgcttcg ctcaactgct gcgcggcggc gtagtgtatc
     1141 gggaagatgt cttcaacgaa ctgccggaag aatggcacgc cgtcctgctc tcgattgata
     1201 cgtttctccg gtcgcccgct gcggacaatg tcgtggagct ttgcccattc cgacagcatc
     1261 cgctcgctgg tcagcaggaa aaacgcccca tgaaatgcgg gctttcccct caccaagaaa
     1321 gcggcgctct cgggtgtgag cgcgtagcgc ccgccgtcgt ccttcgccag caactccaag
     1381 ccgacgagcg cattcaagag cgcacgcaaa ccgcgtaagg atgtttgagt ttcagcgcac
     1441 agtgtctcaa tggtcttggc gccttcgtcg agcgcatcga agactcggtg ccgcatagcg
     1501 gcttcgataa tcaacggcgg cgcaaagccg aaggtgaatt gcatgatgcg ttcaggcgtg
     1561 acctgcgtgg cggacgcggt tgccatgtcg gggcggcgcg cagcggtctg ttgggtggcg
     1621 gggaacgagg tcggatggcg ccgtagcatc accgccgcaa tctagcggcc gcggaccggc
     1681 gggcactccg attcttgctt tcaaatcgaa gatgatgcga gcttgcggcc caagtcagat
     1741 aaagcaacct cagtcatttg atgtggaatc cgccaccgag cgatgatggt gcggctccat
     1801 caatgagggc tcgtcaggcg tcgccggtcg gtacgatcgc cgctgtctcg atcatgagaa
     1861 tcacggcctt ctcgggggcg cgtgtgcggt ggaccacgcg tttcgggact acgaagccct
     1921 gctgcggtga aagcgtgacc gagcggccct cgaggtcaat gatgaaacga ccgctgagca
     1981 cgaaaaagaa ttcgtcgtcg tcatcgtgct tatgccaatg atactcgcct tgcatgacgc
     2041 cgaggcgtac aacggaatcg ttcaccttgc agagcgtctg attataccat tggtcggtgc
     2101 aggcctgcac gagtgaaggc acatcgatga gtttgagcgg atcgaactta atgtcgagat
     2161 atgtcgcata agggaaggcg gcgcccgtgt ccgttgcaga cataggactc tcctttaatc
     2221 gggattcctc gaatgagcac gggcatggat ggtagcaatt ttccggattg gccggaaacc
     2281 taggtgcgcg ttccgatgcc gagatagatt tcctgcacct gttcgttctt ttccaagtcg
     2341 accgccttgc cttcaagcac ggtgcggccg gtctgcagca cataggcgta gtcggcgata
     2401 tcgagcgcga gcggtatcga ctgctcggcc agcaacagcg tcaatccctc cttgtgcagc
     2461 cggtcgaggg tctcataaag cgcttcgacc acgccg
//
LOCUS       DATLJT010001055         3507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1681903,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001055 DATLJT010000000
VERSION     DATLJT010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681903"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..838
                     /locus_tag="VM910_24210"
     CDS             461..838
                     /locus_tag="VM910_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65659.1"
                     /translation="MSRKAAAALAVLLVLGTASAVRADADDRIAASRLAVERSAPLCR
                     DGIAGRSRIWIKLTEPGGKLIHINVEHVTSVRSDTQIPGARAQLDLTSGKFQGVQETI
                     DEIMALISPAPGARENGKADVVL"
     gene            1063..1233
                     /locus_tag="VM910_24215"
     CDS             1063..1233
                     /locus_tag="VM910_24215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65660.1"
                     /translation="MKTLITALALTVLVAAPAFSQSAAAAARKTGRDACRSQQNDQCY
                     YRGYPLWQWYSS"
     gene            1519..1743
                     /locus_tag="VM910_24220"
     CDS             1519..1743
                     /locus_tag="VM910_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65661.1"
                     /translation="MSTSDDVQKRNAFAACAAFRIESLEASLDHAREIAVRDELGAVG
                     HYIEEAQGYLAQIRVLHLEALDEFRRGQGE"
     gene            complement(1849..2169)
                     /locus_tag="VM910_24225"
     CDS             complement(1849..2169)
                     /locus_tag="VM910_24225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65662.1"
                     /translation="MHPARFRWFIIFLLFATSIMGANAVYFAVNFDVAPKHAATAFGI
                     MNLAFAISGFLAPVITGSVLKLHGSFVDAFWLMAALALLSVIVVLVFHDPDRDRQKQM
                     PRAE"
     gene            2396..>3507
                     /locus_tag="VM910_24230"
     CDS             2396..>3507
                     /locus_tag="VM910_24230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65663.1"
                     /translation="MNLLHPSRRQVMGASLIGGIAVYLAPLGSRAFAALFEEQLLTRP
                     TWNDGVLKYRIDGTSKVTGEKVFARDIRARDMPHWPQQQSHALVLRVTMADRSYAGFD
                     LSALGSELAPDRVVTAADLARDGLAFPDFYGEDILLPEGKTPAYLGQAVAILIYQDFA
                     RLRFAKEKLQFIDAVIRYGAVTGPLQRDPWGSYRFVRVAGPTPHDDDVFSCLKQGPLS
                     PSYAQHEPVWPQPARDGDVGAQGMYYAKSIDDELAQPPADWLVLKRDYATQSGDTAAF
                     EPDNANGWYDREKEDLHLVVPTQSPQEVAAGAAAMLGKSRIGLKRLFPHPCFTVGYGS
                     KDHCTMPYYGLVAAVYGDGRPVRLANDRFDQFQTGL"
BASE COUNT          698 a         1099 c         1107 g          603 t
ORIGIN      
        1 ggttcgatgt gaaggaaggc gaagcgtcga ttttcaaacc cgagggtgcc aagtatcagc
       61 tggtcgcgcg cgtgcagatg gaggactggc cgaagctcgc cgttgccgtc cagtgacggg
      121 gcgcactctt ggaagcggcg caggccgcac cagaatcgga agcacccgcg cacgcgtcgc
      181 ggcgcctcaa ttgatcgaat tgcattcgat accctccagc cagggccgct ttgcaggata
      241 tcgaatgggc agcgatcact cagcgggtgc ttcccgagcc aagtccgctt tgccccccac
      301 gcgggacatc gcgggcttgg ccgtgtgatg cccatcacaa aaaagcgctt ttcaccgccg
      361 aactgcgcaa aaagcgccgc gcattgccgg tcgcgccgga ataggcagcg gcctgatgca
      421 atctcatggg acgaaggcgc tttcagcaag gagagcgaaa atgtcccgca aggccgccgc
      481 ggctctggcc gtcctgctcg ttcttggcac cgcctcggcc gttcgagcgg atgccgatga
      541 ccggatcgcc gcctcacggc tcgccgtcga acgcagcgcc ccgctctgcc gtgatggtat
      601 cgcgggcaga tcgaggattt ggatcaaatt gaccgaaccc ggcggcaaac tgattcatat
      661 caatgtggaa cacgtcacgt ccgtcagatc cgacacccaa attcccggcg ccagggctca
      721 actcgacttg accagcggga aatttcaggg cgtgcaggaa acgatcgatg agatcatggc
      781 gttgatctcg cccgccccgg gcgcgcgcga gaatggcaag gcagacgtag tcctgtgatc
      841 gagctgcgag cgccggggcg acatcagctt cgcttgcgca caaggtcgcg gcattcatgc
      901 catccgaagt tggcgatagc ggcttaagtc gatctcccgt cttatgcggg cgagcccgga
      961 ataaacatgg ccgtccctcg atctagtccg cgaagggcga cgtatggatt gctggtatcg
     1021 gcggaccgcc tccgctgctg cgcaatcgat ggagaggcac ccatgaagac gctcatcacc
     1081 gcgctggctt tgactgtgct cgttgccgcc ccggctttca gccagtccgc tgccgccgcc
     1141 gcgcgcaaga ccggccgcga cgcctgtcgg tcgcagcaaa acgaccagtg ttactaccgg
     1201 ggatatcccc tgtggcagtg gtatagcagc tgacggccgc tcaaacgcga gcggctcgca
     1261 agcgaacgct tccgatcggg ctcacgccct gcgttcgaag gcattcagcc cgattcagaa
     1321 cgattcagct ctagcccaag gacctttaag gctgcgctgg gggattatcc tcccgcagcc
     1381 tgagacaggt cacgaagacg ggtcacgaac agaggtcacg tcagcaatgt gccgaggccg
     1441 tgccgacatg gagggagcga aacaagaaga ttgacgcccc gcgcaacgcg aggctccggc
     1501 aaacttgagg atagagccat gtcaaccagc gatgacgtac aaaagcgaaa tgccttcgcc
     1561 gcttgtgctg cgtttcgcat cgagagcctt gaagcctctc tggatcatgc gcgcgagatt
     1621 gcagtccggg acgagctggg tgccgtaggg cactacatcg aagaagcgca aggatacctg
     1681 gctcagattc gggtcttgca tctagaagcg ctggatgagt tcaggcgagg gcagggggaa
     1741 taaggcgctc gctccgcccg cgccggcgga cggctgcggc cgcgcccacg ggtggtggag
     1801 ccgctcgcgc cgtcacgcgg ctgcataaag ccttccacag cgcaagcgtt actcggctcg
     1861 cggcatttgc ttttgtcggt cacgatcggg atcgtgaaag accagcacaa cgataaccga
     1921 cagcaaggcg agagcggcca tcagccagaa cgcgtccaca aacgatccgt gcagcttaag
     1981 caccgagcca gtgatgacgg gcgccagaaa tcccgagatc gcaaaggcga gattcatgat
     2041 gccgaatgcg gtcgccgcat gcttgggggc gacgtcgaag ttgacggcaa aatagaccgc
     2101 attcgcgccc atgatgctgg tcgcaaacag aaggaatatg ataaaccagc ggaagcgggc
     2161 cggatgcatt cgtctttccc tttaggactt gcgtgccggg gaccggcggc gcaagcaaac
     2221 gacggagctc aatgcctcaa aaaccggggc aaccgctcgt ccaaacgctt agcagctgac
     2281 ctcccatgag ttcaaaattg atcgctggcg ctccagcacc ccgccgcgaa tcgaaccgct
     2341 gcgaatcgaa cccctgccgt agcgtgggtt gcgcctgcgc actggagcca tccgcatgaa
     2401 cctcttgcac ccgtcgcgcc ggcaggtgat gggcgcgagc ctgatcggcg gcatcgccgt
     2461 ctatctcgct ccgctcggaa gcagggcctt tgcggcgttg ttcgaagaac aattgctgac
     2521 gcggccgacg tggaacgatg gcgtcctgaa atatcgcatc gatggtacgt cgaaggtcac
     2581 gggagagaaa gtattcgcgc gcgacatccg cgcccgcgac atgccgcatt ggccgcagca
     2641 gcagtcgcat gcgctggtgc tgcgggtgac catggccgat cgcagctatg ccggcttcga
     2701 tctctcggcg ctcggctccg agcttgcgcc cgaccgcgtg gtgacggcgg cggatttggc
     2761 gcgcgacggg cttgcctttc cggattttta cggcgaggac attttgctcc ccgaaggcaa
     2821 gacgccggcc tatctgggcc aagccgtcgc gatcctgatc tatcaggatt tcgcccgctt
     2881 gcgcttcgcc aaggaaaagc tgcagttcat cgacgcggtc attcgctatg gcgccgtgac
     2941 cgggccgctg cagcgcgacc cttggggctc ataccgcttc gtgcgggtcg caggccccac
     3001 gccccacgat gacgacgtgt tttcctgcct gaaacaaggg ccgctctccc ccagctacgc
     3061 gcagcatgaa ccggtctggc cgcagcccgc gcgcgacggc gatgtcggcg cgcagggaat
     3121 gtactacgcc aagtcgatcg atgacgagct ggcgcagccg ccggccgact ggctggtgct
     3181 taagcgcgac tatgccaccc agtcgggcga caccgcagcg ttcgagccgg acaacgccaa
     3241 cggctggtac gaccgcgaga aagaggactt gcatctcgtc gtgccgacgc agtcgccgca
     3301 agaagtcgcg gcgggcgcgg cggctatgct gggaaaaagc cgcatcggcc tgaagcggct
     3361 gttcccgcac ccatgtttca cggtcggcta cggctcgaag gatcactgca ccatgccgta
     3421 ttacggcctc gtcgccgcgg tttacggcga cggccggccg gtgcggctcg ccaacgatcg
     3481 gttcgatcag tttcagactg gcctgaa
//
LOCUS       DATLJT010001056         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_73164,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001056 DATLJT010000000
VERSION     DATLJT010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73164"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VM910_24235"
     CDS             complement(<1..834)
                     /locus_tag="VM910_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65664.1"
                     /translation="MNDGLPEPVTTASAPRSAAVAFIFVTILLDTLALGVIIPILPKL
                     IESFVSNDTASAARVFGLFGTAWALMQFIFSPVLGSLSDRFGRRPVVLLSNFGLSADY
                     VLMALAPSLAWLFIGRLISGITSASISTAFAYIADGTPPEKRAEVFGRIGVAFGAGFI
                     LGPALGGLLGDIAPRLPFWTAAGFSLANGLYGLFILPESLPRERRAAFRWKSANPIGM
                     LPLLRSSRTLAGLQAANFFAQLAHVVLPSTFVLYASYRYGWDAKTVGLTLAIVGICAM
                     IV"
     gene            complement(831..2201)
                     /gene="der"
                     /locus_tag="VM910_24240"
     CDS             complement(831..2201)
                     /gene="der"
                     /locus_tag="VM910_24240"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVI65665.1"
                     /translation="MSFTIAIIGRPNVGKSTLFNRLVGQKLALVDDLPGVTRDRREGE
                     ARLADLAFTVIDTAGLDEGAKGSLTARMQEQTETAIAQADALFFVIDARAGLTPTDRA
                     FADFARRADKPVLLVANKSEGKHGDAGAMEAYALGLGDPIQISAEHGEGMGELYDALR
                     ALMPEPPEDAEASEADEDEDSSRPIRVAIVGRPNAGKSTLINHLIGEERLLTSPEAGT
                     TRDSIAVDIDWKGHFFRLFDTAGLRRRSRIEEKLEKLSVADALRAVRFAEVVVLLMDA
                     QNRFEEQDLRIADLIEREGRALVIAVNKWDLVPGKPGQISALRADADHWLPQVAGVPI
                     VAVSGLMGEGIDRLMQAILDAYAIWNKRVPTSALNRWFEQAIAANPPPAVSGRRLKLN
                     YITQTKARPPSFVLFCSRADAVPQSYLRYLTNSLREAFALPGTPVRITLREKQNPFAH
                     KRKRLS"
BASE COUNT          415 a          721 c          738 g          361 t
ORIGIN      
        1 cacgatcatc gcgcagatgc cgacgatcgc gagcgtcagt cccaccgtct tggcgtccca
       61 gccatagcga taggacgcgt acagcacgaa ggtggacggc agcacgacat gcgcgagctg
      121 ggcaaagaaa ttcgcggcct gaagtcccgc gagcgtccgg ctcgagcgca aaagcggcaa
      181 catgccgatc gggttggcac ttttccagcg gaacgccgca cggcgttccc tcggtagcga
      241 ttccggcaag atgaagaggc catagagccc gttggcgagg ctgaagcccg ccgcagtcca
      301 gaacggcagc cgcggcgcga tgtcgccaag caggccgccg agcgccggcc ccagaatgaa
      361 accggcaccg aacgccaccc cgatcctgcc gaacacttcc gcgcgttttt ccggcggcgt
      421 cccgtccgcg atataggcga aagcagtcga gatgctcgcc gaggtgatgc ccgagatcag
      481 ccggccgatg aagagccagg ccagcgatgg tgcgagcgcc atcagcacgt agtccgccga
      541 cagcccgaaa ttcgacaaca gaaccacagg gcgccgcccg aaccggtcgg acaggctgcc
      601 gagcacgggc gagaagatga attgcatcaa cgcccaggcc gtgccgaaca ggccgaagac
      661 gcgcgcggcg ctcgcggtgt cgtttgagac aaagctctcg atcagtttcg gcaggatcgg
      721 aatgatgacg ccgagcgcca gcgtatcgag caggatggtg acaaagatga aggcgaccgc
      781 ggcgctacgg ggcgccgatg ccgtggttac gggctccggg aggccgtcgt tcacgacaga
      841 cgtttccgct tgtgcgcaaa cggattttgc ttctcgcgca acgtgatcct gaccggcgtg
      901 cccggaagcg cgaacgcctc gcgaagcgag ttggtgagat agcgcaggta ggattgcggt
      961 accgcatccg cacgcgagca gaacagcacg aagcttggcg gccgcgcctt ggtctgggtg
     1021 atgtagttca gcttcaaacg ccgtcccgac acggcgggcg gcggattggc tgcgatcgcc
     1081 tgttcgaacc agcgattgag cgctgaagtc ggcacgcgct tgttccagat ggcgtaggcg
     1141 tcgaggatcg cctgcatcag gcggtcgatg ccctcgccca tcaaaccgga tacggcgacg
     1201 atcggcacgc ccgcaacctg cggcaaccag tggtcggcat cggcgcgcag cgccgaaatc
     1261 tgcccaggct tgcccggtac gagatcccat ttgttgacgg cgatcacgag tgcccgtccc
     1321 tcgcgctcga tcaggtcggc gatgcgcaga tcctgctcct cgaatctgtt ctgcgcgtcc
     1381 atcagcagca cgacgacctc ggcaaagcgt accgcgcgca acgcatccgc gaccgagagt
     1441 ttttccagct tctcctcgat ccgcgagcgg cgccgcaggc ccgcggtgtc gaacaggcga
     1501 aagaagtgac ccttccagtc gatatcgacc gcgatcgaat cgcgggtcgt gccggcttca
     1561 ggactggtca agagacgttc ctcgccgatg aggtggttga tgagggtcga tttaccggca
     1621 ttgggccggc cgacgatggc gacgcggatg gggcgcgagg aatcctcgtc ctcgtcggcc
     1681 tctgaagctt cggcgtcttc cggcggctcc ggcatcagcg cacgcaaggc gtcgtagagt
     1741 tcgcccatgc cctcgccatg ctccgccgaa atctggatgg gatcgccgag cccgagcgcg
     1801 taggcctcca tcgctcccgc gtcgccgtgc ttgccctcac tcttgttggc gaccagcagc
     1861 acgggtttgt cggcccggcg tgcgaaatcg gcaaaggcgc gatcggtggg cgtgaggccg
     1921 gcgcgcgcat cgatcacgaa gaacagcgcg tcggcctgcg cgatcgcggt ctcggtctgc
     1981 tcctgcatgc gtgccgtcag cgagcccttg gcgccctcgt cgagaccggc ggtatcgatc
     2041 accgtgaatg cgaggtcggc gagcctggcc tcgccctcgc gccggtcgcg cgtcacgccg
     2101 ggcagatcgt cgacgagcgc gagcttctgt cccaccaggc ggttgaacag cgtcgatttt
     2161 ccgacattgg gccggccgat aatcgcgatc gtgaaagaca tgggtgatcc ctgcgcattt
     2221 cagagcgcgc atgtc
//
LOCUS       DATLJT010001057         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1222233,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001057 DATLJT010000000
VERSION     DATLJT010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222233"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VM910_24245"
     CDS             <1..838
                     /locus_tag="VM910_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin"
                     /protein_id="HVI65666.1"
                     /translation="ASMNYGTAVVNFNNANPFTANGAPLVSGNAFSASSILKGLPTVT
                     ATIRAMENAGVVHTLAEPNLTAISGEAATFIAGGEFPIPGGYSCDPTTHVCTTQITYK
                     KFGISLNFTPVVLSEGRISLRVMTEVSELSNDNAITVSQALTANTTNSIVIPSIRTRR
                     AETTLEIPSGGSMAMAGLIQQQTKQAINGLPGLDQVPVLGQLFRSQDYVNNETELMVL
                     VTPYVVRAVAQKELSRPDDGFAPASDSQSALLARINRIYGVAARVEPIGGYQGSFGFI
                     ID"
     gene            878..1618
                     /locus_tag="VM910_24250"
     CDS             878..1618
                     /locus_tag="VM910_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HVI65667.1"
                     /translation="MTNGQPLDRLRTPRLLGALVGLSVALGACTYAPEAIITGGAPDD
                     YRLRHPIAITEANRSIVVFVGHARGGLTVAQRSDVIGLARTWLSEGTGAILVEVPVDT
                     PNARAAEATFHEIRGLLLASGVPGRGITLQRYHPIDPQTLATIKLTYSRIAAVAGPCG
                     LWPEDLGPSINNPAYNENKPYFNLGCATQRNLAAMVDNPSDLVQPRSETPPYTPRRSE
                     AFEKYRKGEATTTTYPEAEKAKLSDTGK"
     gene            1615..>2313
                     /locus_tag="VM910_24255"
     CDS             1615..>2313
                     /locus_tag="VM910_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtpF protein"
                     /protein_id="HVI65668.1"
                     /translation="MTRQSPKDSEELPDTAPPAANDYIAAAPRISVQAFCVTEDTAAA
                     VQSAGEDRRLGKAHLTVKMGGMGGAVDAYHNAPTPNVIVIESEGQGDILAGLDELASV
                     CDPGTRVILVGSENDEVPYRELVRRGVNDYVIGPVSPLDVVRSICSLFSASEAITVGR
                     VIAIAGAKGGVGASTIAHNVAWAIARNLKLDSVVIDLDLAFGTASLDYNQDPTQGIAN
                     AVFQPDRPDSAFIDR"
BASE COUNT          447 a          794 c          684 g          388 t
ORIGIN      
        1 cgccagcatg aactacggca ccgcggtcgt gaacttcaac aacgccaatc cgttcaccgc
       61 aaatggcgcc cctcttgtgt ccggcaacgc cttctccgct tcctccatat tgaagggctt
      121 gccgacggtc accgcgacca tccgcgcgat ggaaaacgcg ggcgtggtgc acactctcgc
      181 cgagccgaat ctgactgcaa tctccggcga ggcggcgacc ttcatcgcag gcggcgagtt
      241 cccgattccg ggcggctact cctgcgatcc caccacgcat gtctgcacga cgcagatcac
      301 ctacaagaaa ttcggcatct cgctcaattt cacccccgtc gtgctgagcg aaggacgcat
      361 cagcctgcgg gtcatgaccg aagtctcgga gctctccaac gacaacgcga tcacggtatc
      421 tcaggcgctc accgccaaca cgaccaactc catcgtgatc ccctcgatta ggacgcgccg
      481 cgccgaaacg accctggaaa ttccctccgg tggctccatg gccatggccg gcctgattca
      541 gcagcagacc aagcaagcga tcaacggcct gcccgggctc gatcaggtgc cggtgctggg
      601 ccagctcttc agaagccagg actacgtcaa caacgaaacc gaactgatgg ttctggtgac
      661 gccctatgtc gttcgagcgg tcgcccagaa agagctgtct cgtccggacg acgggtttgc
      721 gccggcatct gattctcaat cggcgctgct ggcccgcatc aatcgtatct acggcgtggc
      781 cgcccgcgtc gaaccgatcg ggggctacca gggcagtttc ggcttcatca tcgactgata
      841 cgccgcggcg caaggcaagg ggatggggga catcacgatg acaaacggac agccgctcga
      901 tcgccttcgg accccgcgcc tgctgggcgc gttggtcggc ctctccgtcg ccctcggcgc
      961 gtgcacctat gcgcccgaag cgatcatcac gggcggcgct cccgacgatt accgcttgcg
     1021 tcacccgatc gcgatcacgg aggccaaccg ttcgatcgtc gttttcgtcg gccatgcgcg
     1081 cggaggcctc acggtcgcgc agcgctccga cgtgatcggc ctggcgcgca cctggctttc
     1141 ggaaggcacc ggtgcgatcc tggtggaggt gccggtggac acgccgaacg cccgcgccgc
     1201 ggaagccaca ttccacgaaa ttcgcggact gctcctcgcc agcggcgtgc cggggcgcgg
     1261 catcacgctg cagcgctacc atccgatcga tccgcagacg cttgccacga tcaagctgac
     1321 ctattcgagg atcgctgcgg tggcgggtcc ttgcgggttg tggccggagg atctcggccc
     1381 ctcgattaat aatccggcct acaacgagaa caaaccgtac ttcaatctcg gctgcgcgac
     1441 ccagcgcaat ctcgcggcga tggtcgacaa cccgtccgat ctggtgcagc cgcgttccga
     1501 aacgccgccc tacacacccc gcaggagcga ggcgttcgag aagtatcgca agggcgaagc
     1561 gacaacgacc acctatcccg aagccgaaaa ggccaaactc agcgatacag gcaaatgacc
     1621 cgccagtccc caaaagattc cgaagagctg ccggacaccg cgccgccagc ggccaacgac
     1681 tatatcgccg ccgcgccccg tatctcggtt caagccttct gcgtgacaga ggataccgcc
     1741 gccgccgtgc agtcggcagg agaggaccgc cgcctcggca aagcgcatct caccgtcaag
     1801 atgggcggca tgggtggggc cgtcgatgcc tatcacaatg cgccgacccc gaacgtcatt
     1861 gtgatcgaga gcgaagggca gggcgacatt ctcgccggcc tcgacgaact ggccagcgtt
     1921 tgcgatcccg gaacacgcgt gatcctcgtc ggcagcgaaa acgatgaggt gccttatcgc
     1981 gagctggtgc gccgcggcgt caacgattac gtaatcgggc cagtcagccc gctcgacgtg
     2041 gtgcgttcga tctgcagcct gttctccgcc tccgaagcga tcaccgtggg ccgcgtcatc
     2101 gccattgctg gagccaaggg cggcgtcggt gcgtccacca ttgcgcacaa tgtcgcctgg
     2161 gcgattgcac gcaacctcaa gctcgactcg gtcgtgatcg atctggacct cgccttcggc
     2221 acggccagcc tcgactacaa ccaggatcca acgcaaggaa ttgccaacgc ggtgttccag
     2281 ccggaccggc cggatagtgc gttcattgac cgc
//
LOCUS       DATLJT010001058         5248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3060578,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001058 DATLJT010000000
VERSION     DATLJT010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060578"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VM910_24260"
     CDS             complement(<1..660)
                     /locus_tag="VM910_24260"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI65669.1"
                     /translation="MSSDAPAYGLWGLVIVNSVVFILFAYSFFKPRTGRDWRSFGAFS
                     AFLVALFAEMYGFPLSIYFLSGWLQSRYPGIDWFSHDAGHLLEMMFGWKTNPHFGPFH
                     ILSFVLIGGGFVLIAAAWRVLFDAQHRHTLATSGPYSHIRHPQYVGFILVMLGFLVQW
                     PTLLTLAMFPVLTVMYVRLAKREEREAITAFGDAYVKYMSEVPGFIPRISRFLGQPNR
                     TG"
     gene            complement(657..905)
                     /locus_tag="VM910_24265"
     CDS             complement(657..905)
                     /locus_tag="VM910_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HVI65670.1"
                     /translation="MPMSLHEHSGREQGSFLTLPAGLVLIGFLMIAGVLLFTEHRAHV
                     LGALLYVPLLLCPLMHLFMHGHGGHGGHGQDDREGRAS"
     gene            complement(1041..1226)
                     /locus_tag="VM910_24270"
     CDS             complement(1041..1226)
                     /locus_tag="VM910_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65671.1"
                     /translation="MRVFLTSVFAAIVIAGAAMVVLDQSWQQADEAFVSPTSVRIPSH
                     GNTHNLVGKTWFSGKDH"
     gene            1357..2721
                     /locus_tag="VM910_24275"
     CDS             1357..2721
                     /locus_tag="VM910_24275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI65672.1"
                     /translation="MGLNLSFLGGAGTVTGSKYLVEHEKHKLLVDCGLFQGFKALRLK
                     NWAPFPVEPGSIDVVVLTHAHLDHCGYLPLLIKRGFRGPVYCSRATRDLCAILLPDAG
                     HLQEKDAEFANRHGFSKHKPALPLYTLEDARAALEHLTPIPFDQERTLPGGGTVRLRR
                     SGHILGAGWVQLDWAGITTVFSGDLGRYEDPVLVDPMSVERADYLLVESTYGDRRHDK
                     RDPAEALAEIVGSTIARGGTVIIPAFAVGRVQSLLFHFHQLKLKGQLSNVPIFLDSPM
                     ALDASEIFCKDINDHKLSEAECRRACGVAHYIRSVEESKALTANPVPKVIISASGMAT
                     GGRVLHHLKRYAPHPKNTILFAGFQAGGTRGAAMLSGVESVKIHGEYLPVRAQVNNRD
                     MLSAHADADEIMRWLRGFKAAPRMTFVTHGEPAASDALRHRIEEELRWPCMVPDHGQR
                     VDLA"
     gene            2718..4262
                     /locus_tag="VM910_24280"
     CDS             2718..4262
                     /locus_tag="VM910_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008282885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HVI65673.1"
                     /translation="MNDGQHRPSGARERTATNSVRTRRLGLDTQYEAVVFMHKECQVC
                     RSEGFSAHARVLLCNGNRQVIATLYQVTGDLVGHDEAALSESAWSRLGLTEGDRIIVT
                     HPAPLDSLSHVRRRIYGHELSESSFHSIVQDVVDGKYSDIHLSSFLTACAARALDHKE
                     ILALTHAMVEVGERLKWPAGVAADKHSAGGLPGNRTTPIIVAIAASLGLTMPKTSSRA
                     ITSPAGTADTMETMAPVELDVAAIRRVVEREGGCVVWGGAVKLSPADDILIRVERALD
                     LDTEGQLIASVLSKKIAAGSTHLVVDLPVGPTAKVRSSESAEVLARDLVSVAEAFGLH
                     TKVVLTDGTQPVGRGIGPALEARDVLAVLQCKPDAPQDLRRRAIALAGTLLELAGAAS
                     EGGGEATATKALDDRRAWQKFQCICEAQGGMRAPPTSRQQRPLLAERSGRVHAIDNRR
                     IARLAKLAGAPDDKAAGVDLRVAVGDPVASGQPLCTVHAEAPGELAYAFDYAAANRDI
                     IAIRES"
     gene            4259..5146
                     /locus_tag="VM910_24285"
     CDS             4259..5146
                     /locus_tag="VM910_24285"
                     /inference="COORDINATES: protein motif:HMM:NF005537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVI65674.1"
                     /translation="MTVPLVIAMPGNEAMAQMLTRSLRGDIGGIELRSFPDGETYLRF
                     LSHLSGRVLVIACTLDRPNEKILPLLFAAATARELDAEKVGLVAPYLAYMRQDRRFKP
                     GEAVTSRQIAQLLSNAFDWLVTVDPHLHRHGSLAEIYRIPTRVVHAASLISRWIKMHV
                     PKPFVIGPDSESEQWVAAVAKDSGAPYAVLQKVRRGDRDVEISAASLPDLDGRTPVLV
                     DDIISSGRTMIAAVRLIVNREALTPVCVAVHGLFADHSDQILARAGARVVTSNSIPTQ
                     PTALTWRKPWETRSVSFLS"
BASE COUNT          994 a         1634 c         1623 g          997 t
ORIGIN      
        1 tcctgtccta ttcggttggc cgaggaaccg gctaatccgg gggatgaatc ccggcacctc
       61 gctcatatat ttgacgtagg catcgccaaa tgcggtgatt gcttcacgct cttcgcgctt
      121 tgccaacctg acgtacatca cggtgagcac cggaaacatc gccagtgtca gcagcgtcgg
      181 ccactgcacg aggaagccga gcatcaccaa gatgaagccg acgtattgcg ggtggcggat
      241 gtggctatat ggtccgcttg tcgcgagggt atgacggtgc tgggcatcga agagcacgcg
      301 ccaggccgcg gcgatcagca cgaaacctcc gccgatcaac acgaaactca aaatatgaaa
      361 cggaccgaag tgcgggttcg tcttccagcc gaacatcatc tcgagcaggt gtccggcgtc
      421 gtgcgagaac cagtcgatgc ccggatatcg cgactgcagc cagcccgaaa ggaaatagat
      481 gctcagcgga aagccgtaca tctcggcgaa cagcgcgacc aggaacgcgc tgaaggcgcc
      541 gaacgagcgc cagtcccggc ccgtccgcgg cttgaagaag ctgtaggcga acaggatgaa
      601 gaccacggag ttgacgatga cgaggcccca caggccgtag gcgggcgcgt ctgagctcat
      661 gacgccctcc cttcacgatc gtcttgccca tgacctccat gaccaccgtg gccatgcatg
      721 aagaggtgca tgagcgggca aaggaggagc ggcacataaa ggagtgcacc caggacgtgg
      781 gcgcgatgct ccgtgaatag cagtacaccg gcaatcatga ggaacccgat gagaaccagt
      841 cccgccggca aggtgagaaa gctaccttgc tcacgaccac tgtgctcgtg cagcgacatt
      901 ggcatccctc cgctcttatc cggcggcatc cgcctaggag ttccggtcgg cggacgttgc
      961 tgcccaggac tgatttgagc cgggcccgct gtccggctgg cgtccgtccc tcgatcgtac
     1021 cttcgttctg cccgccgacg tcagtgatcc ttgcccgaga accaggtctt cccgacgaga
     1081 ttgtgggtat tgccatgact cggaatgcgc acgctcgtcg gtgacacaaa ggcttcatcc
     1141 gcttgctgcc acgactggtc cagaaccacc atcgctgccc ctgcgatgac gattgccgcg
     1201 aagacagagg tcaggaacac gcgcatgctc acctcccaat tcgcctaatt gcctcgaata
     1261 atcgagagcg ccttcccgga tattgacgtg gatcaaataa gcggtccgcc aacaagttag
     1321 tcctatccgg ccgccaaaat tggaaaggca tcggcgttgg gtctcaacct ctccttcctc
     1381 ggcggcgccg gtacggtgac tggatcgaaa tatttggtgg agcacgaaaa gcataagctg
     1441 ctcgtggact gcggcctgtt tcaaggcttc aaggcgctgc gcctcaagaa ctgggcgcct
     1501 ttcccggtcg aaccgggcag catcgatgtc gtcgttctca cccacgccca tctcgaccac
     1561 tgcggctatc tgccgttgtt gatcaagcgc ggcttccgcg gacccgttta ctgctcgcgc
     1621 gcgacgcgcg atctatgcgc gatcctcctg cccgacgccg gccacctgca ggagaaagac
     1681 gcggaatttg ccaaccgtca cggcttctcg aagcacaagc ctgcgttgcc cctctacacc
     1741 ctggaggatg cccgcgccgc tctcgagcat ttgacgccga tcccgttcga tcaggagcgg
     1801 acattgccgg gcggaggcac ggtgcggctg cgccggagcg gccatattct cggtgctgga
     1861 tgggttcagc tcgattgggc cggcatcacg accgtcttct ccggtgatct cggtcgatat
     1921 gaagaccccg tcttggttga tcccatgtcg gtcgagcggg ccgactatct gttggtcgag
     1981 tcgacttacg gtgatcggcg acacgacaag cgcgatccgg cagaggcgct ggcggagatt
     2041 gtcggcagca cgattgcccg tggcggaacg gtgatcattc cggcgttcgc ggtggggcgg
     2101 gtgcaaagtc tgctatttca ctttcaccaa ttgaaattga agggccagct gtcgaacgtt
     2161 ccgatcttcc tcgatagtcc aatggcgcta gatgccagcg agattttctg taaggacatc
     2221 aacgaccata agctttccga ggctgagtgt cggcgcgcct gtggcgttgc gcattacatc
     2281 cgcagcgtcg aagaatcgaa ggcgctgacc gccaatccgg tgccgaaggt catcatttcg
     2341 gcaagcggga tggcgaccgg cggacgcgtg ttgcaccatc tcaagcgcta tgcgccccac
     2401 cccaagaaca ccattctgtt cgccgggttt caggccggcg gaacccgcgg tgctgccatg
     2461 ctgtcggggg tggaaagcgt caagatacac ggcgaatatt tgccggttcg tgcgcaggtg
     2521 aacaatcgcg acatgttgtc cgcccatgcc gatgcggacg agatcatgcg ttggctccgc
     2581 ggcttcaagg cagcgccccg aatgacgttc gtgacccatg gcgaacctgc ggcgtccgac
     2641 gcgttgcgcc accgaatcga ggaggagctt cggtggccgt gcatggtccc cgatcatggt
     2701 caaagggtcg acctcgcatg aatgatggac aacacagacc gtcgggtgcg cgcgagcgaa
     2761 cggcgacgaa cagcgttcgc actcgccgcc ttggcctcga tacccaatat gaggcggtcg
     2821 tgttcatgca caaggagtgt caggtctgcc gctccgaagg attcagcgcg cacgcgcgag
     2881 tcttgctgtg caatggcaac cgacaggtca tcgccacgtt gtatcaagtg acgggcgacc
     2941 tggtcgggca cgatgaggcc gcgctgtccg agtccgcctg gtcgcggctt ggactgacgg
     3001 agggcgatcg catcatcgtt actcaccctg ctccactcga ttctctcagt catgttcgca
     3061 ggcgtatcta cggacacgag ctcagcgaat catcgttcca ttccatcgta caggacgtcg
     3121 tggacggcaa atattccgac attcatctct cttcgtttct caccgcctgt gccgcccgcg
     3181 cgctcgatca taaggagatt cttgcgctga cccacgcgat ggtagaagtg ggtgagcggc
     3241 tgaaatggcc ggccggagtg gccgcggaca aacactccgc cggcggactt cccggtaatc
     3301 gcaccacccc gatcatcgtg gcgatcgccg catcgctcgg cctcacgatg ccgaagacgt
     3361 cgtcgcgggc gatcacgtcg ccggccggaa ccgccgatac catggagacc atggccccgg
     3421 tcgagctgga tgtggcggcc attcgccgcg tggtcgagcg tgagggtgga tgcgtggtat
     3481 ggggtggcgc ggtcaagctc agcccggccg atgatattct cattcgggtc gaacgggcgc
     3541 tcgatctcga taccgaaggg cagctgatcg cgtccgtgct atcgaagaag atcgcggcag
     3601 gctcgaccca ccttgtcgtc gaccttcccg tcggcccgac ggcgaaggtg cgcagttccg
     3661 aaagtgccga ggtcctggca cgcgatttgg tcagtgtcgc cgaggcattc ggactgcaca
     3721 ccaaggtcgt gctgaccgat ggcacgcaac cggtcggccg cggcatcggg cccgccctgg
     3781 aggcacgcga tgtgctggct gtgttgcaat gcaagccgga tgctccgcag gacttgcggc
     3841 ggcgggccat cgcgctcgca ggcacgttgc tcgagctcgc cggtgccgca tccgaaggcg
     3901 gtggcgaggc aacggcgacg aaggcgctcg acgatcgtcg cgcctggcag aagtttcagt
     3961 gcatctgcga ggcccagggt ggaatgcgcg cgccgccgac gtcgcgccag caacggccgc
     4021 tcttggccga acgatcggga cgcgtgcatg ccatcgacaa tcgcaggatc gcccgcttgg
     4081 ccaagctcgc cggcgcgccc gacgacaagg cggcgggcgt cgaccttcgt gtcgcggtcg
     4141 gggatcccgt cgcatccggc cagccgctgt gcaccgttca tgccgaggcg cccggtgaat
     4201 tggcatatgc gttcgactat gcggcggcaa atcgcgatat cattgcgatc cgcgagtcat
     4261 gaccgtcccg ctcgtcatcg cgatgccagg caacgaagcg atggcacaga tgcttacccg
     4321 atcactccgc ggcgatatcg gtggcatcga gttgcgttca tttcccgatg gtgaaactta
     4381 cttgcggttt ctctcccatc tctcgggacg ggtgcttgtg atcgcctgca cacttgatcg
     4441 gccgaatgaa aaaattctgc cgttgctgtt cgcggctgca acggcccgcg agctggacgc
     4501 agaaaaggtc ggcctggttg ccccatatct cgcctacatg cggcaagatc ggcgcttcaa
     4561 gccgggagaa gccgtgacgt cgcgtcaaat cgcccagctc ctgtcgaacg ccttcgactg
     4621 gctggtgacg gtcgatccgc atctccaccg ccatggctcg ctcgccgaga tttaccgcat
     4681 cccaacccgc gttgtgcacg ccgcgtcgct gatttcgcgc tggatcaaga tgcatgtccc
     4741 gaagccgttc gtcatcggtc ccgacagcga aagcgagcag tgggtagcgg cggtcgcgaa
     4801 agattctggc gcgccctatg cggttctgca aaaggttcgc cgcggcgatc gcgatgtcga
     4861 gatctccgcc gcgagcttgc ccgacctcga cggacggacg ccggtactgg tcgatgacat
     4921 catttcgagc ggacgaacga tgatcgcggc cgttcggctc atcgtgaacc gggaagcgtt
     4981 gactccggtt tgtgttgccg tccacggctt gttcgccgac cattccgacc agattctcgc
     5041 gcgcgccggt gcccgcgtgg tcacgtcaaa cagcatcccc acgcaaccaa cggcattgac
     5101 gtggcggaag ccttgggaaa ctcgatcagt gagcttctta agctaacctc taaacggtaa
     5161 gagcgcgaca gccccgcagt cagcgctcgg cgcggctctt gtcgcggtcc gtagcctttc
     5221 tttcccgtgg cgaaccatgc cttgcgat
//
LOCUS       DATLJT010001059         6707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3060638,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001059 DATLJT010000000
VERSION     DATLJT010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060638"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VM910_24290"
     CDS             <1..765
                     /locus_tag="VM910_24290"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65675.1"
                     /translation="DVHVTGDANAMRVLLSGNADIGLIGTLNVLASINAGASIRAVHS
                     WQPIGDYSLVLATGKGSELADVAGKTFASSGPGGLPDQLPRLIMRKHGIDETTARFVQ
                     IGGHAARLQAVIGGRADATLVNTVTALKGVEDGKVTVVTRLSAEFPGLGYVWNVVRTE
                     ALAKPELAGAFQTMTDIGIRGSRFIMANPDEAAAILHERLGDLDLAFLTAVVRDLNGE
                     NVWGVNGGLDPAIEEFTADLNVKLGNLPVAARFKST"
     gene            1118..1465
                     /locus_tag="VM910_24295"
     CDS             1118..1465
                     /locus_tag="VM910_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65676.1"
                     /translation="MKKKTCRLFHVTTATTAAAILAEGFRDGSGGYGTAEEFTGVWLS
                     DRPLDVNEGAEGDTALMISFSIPLSALANYEWIEEGKGYREWLVPAEIIGRHATVSIV
                     GPRHRIPKLRPIA"
     gene            complement(1518..1694)
                     /locus_tag="VM910_24300"
     CDS             complement(1518..1694)
                     /locus_tag="VM910_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65677.1"
                     /translation="MTINDRRATAEFLAALVEIAIAHGRRHYSKSGEPLATTLAVLEC
                     LQREGFVTIEAPVP"
     gene            complement(1691..2386)
                     /locus_tag="VM910_24305"
     CDS             complement(1691..2386)
                     /locus_tag="VM910_24305"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HVI65678.1"
                     /translation="MAGDSREPLTRGIIIEGVIMRRRDAGLIAAIEAVGSVGALARAL
                     GISDKAVRDWFCVPVGRVLQVEAVTGIPRKALRSDLYPANVELDEHADAIRRIDCQSF
                     EGAVEIEIGRRLVLCRKLFKVRRAWSAWIKIEFGWSRKHADNIIGLYLNERDRRICEG
                     GNLVHRAAAEGFVEWVAGVLPALPAPAAKIAAVLPAKHKVDEDVERVMRFLDEFRRAR
                     REARAEKLKWLRA"
     gene            complement(2537..2839)
                     /locus_tag="VM910_24310"
     CDS             complement(2537..2839)
                     /locus_tag="VM910_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65679.1"
                     /translation="MARKRAAERWAEQWAEHRRRHDEEERRQRVMVITEFDAGNPEAT
                     MHALAAEILRFRLCVRQLADAIELTRAGAGFGLIRPGPPRRSQHTPEVPDGVPPAA"
     gene            2900..3694
                     /locus_tag="VM910_24315"
     CDS             2900..3694
                     /locus_tag="VM910_24315"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HVI65680.1"
                     /translation="MLQYHDLLERILADGVEKHDRTGTGTVSVFGHQMRFDLARGFPL
                     TTTKKLHFKSIAHELLWFLAGDTNVEYLHEHSVHIWDKWANANGELGPIYGRQWRAWP
                     TPDGGAIDQITKVVSAIRRNPDSRRHIGTAWNPAEVDKMAVPPCHCLFQFYVANGRLS
                     CQLYQRSADVFIGVPFNIASYALLTLMMAQVAGLKPGEFVHTLGDAHLYHNHLEQAQL
                     QLSRPPRQLPTVTLNPAVTDLLAFRFKDFSLRDYDPHPHIEAEVAV"
     gene            4076..4813
                     /locus_tag="VM910_24320"
     CDS             4076..4813
                     /locus_tag="VM910_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65681.1"
                     /translation="MPKFRADYDVKGSHVLPPDSKPIILRGGAPPFEMKFRNADPDKS
                     GHAPHLIVEVIADSASIDDVANEFRSLLAAQLDILSFVTQSTFVIDQCRRVVEWEPFQ
                     KTREYRPVRRFDPLYPPSPDLRQFLFDAAQIILQAKPPDYVLQALHYFRLGLFEPQPE
                     DQFMHFWRAIETIAEGAKETTRIPIPCPKCSGELFCAIATRCPDEGRYRGKRFASCSS
                     ESSRLRLVYMQLSWGHVTTCSTAGHRT"
     gene            4813..5073
                     /locus_tag="VM910_24325"
     CDS             4813..5073
                     /locus_tag="VM910_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65682.1"
                     /translation="METEIGRPLGAVVNDAGVTAWSVIQHSIPPLGTQIKVMQRGGPF
                     ANYMLTASPVMTFTYSTDAPHPTEDEMPKVEVNLVTTFTDQA"
     gene            complement(5528..6016)
                     /locus_tag="VM910_24330"
     CDS             complement(5528..6016)
                     /locus_tag="VM910_24330"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HVI65683.1"
                     /translation="MYWACAQLEPQRERLAVHCLGLNGFEIYCPRIREQLRRHGRKIV
                     RTPPLFPGYAFVLVVSAWWSARWSPGVRRLVMDGEQPARVPDAVIAEIRSRERNGFVE
                     LPRPRGFVPGMRVKVVSGPLQNQIGMLAILRPHERVLVLLRLLGGEQRVELARNAIEA
                     IE"
     gene            complement(6026..6331)
                     /locus_tag="VM910_24335"
     CDS             complement(6026..6331)
                     /locus_tag="VM910_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65684.1"
                     /translation="MSTIPDDVEQLISRLASPLSPPDRAAFRAAAEDALTRVPCWGEG
                     AVYRAVASLQRAFFDPPTDGRAHWDIEQELRPTKLKSAPPIEYGGDLRHVRYRKRAR"
BASE COUNT         1345 a         2150 c         1973 g         1239 t
ORIGIN      
        1 gatgtgcatg tcaccggcga tgccaacgcc atgcgcgtgc tgctttccgg caacgccgat
       61 atcggcctca tcggcacgct caacgtgctg gcgtcgatca atgccggcgc cagcatccgc
      121 gccgtgcatt cctggcagcc gatcggcgat tacagcctcg tgctggcgac gggcaagggg
      181 agcgagcttg ccgatgtcgc cggcaagacc ttcgcatcgt ccggtcccgg cggtttgccc
      241 gatcagctcc cgcggctcat catgcgcaag cacggcatcg acgaaacgac ggcgcgtttc
      301 gtgcagatcg gcggccacgc tgcccgtctg caagcggtga tcggcggtcg cgccgatgcg
      361 acgctggtca ataccgtcac cgcgctcaag ggcgtagagg atggcaaggt gacggtggtg
      421 acgaggctat cggcggaatt ccccggtctc gggtacgtct ggaacgtggt gcgcacggag
      481 gcgctcgcca agcccgagct tgccggcgcc ttccagacca tgaccgacat cggcatccgc
      541 gggtcgcgct tcatcatggc caatcccgat gaggccgcgg cgatcctgca cgagcggctc
      601 ggcgatctcg acctggcgtt tctcacggcg gtggtgcgcg atctcaacgg cgagaatgtc
      661 tggggggtga acggcgggct cgatccggcg atcgaggaat tcaccgctga tctcaacgtg
      721 aagctcggca atctgccggt cgcggcgcgc ttcaaatcaa cttaagatat tgaagtacaa
      781 catatatctt accgccgccc cacccgccgc ttgtcgcatc cgtgcaaatc cgtaccaggc
      841 aatatcaagc acttataggg tgtgcaattg tgtcgtggca acatgaatgc aacatgccgc
      901 aggcggcgcg acgtcgggca atgcgtcagc gcagggcgtg taggatcagt cagaaacgcc
      961 agcggccccg gcgtggatgg agacccgccg ggaccgctat gccttagccc gaactgcaac
     1021 ccttgaaggg tagcgacgtg aatagtacgt gctcgcgcgg caaccgagca agctttagga
     1081 ggacggcaaa gtgggggcgc cgcgtgggga agtcacgatg aagaaaaaga cgtgccgatt
     1141 gttccacgtg actaccgcaa ccaccgcagc agctattctt gccgaggggt ttcgcgacgg
     1201 aagcggcggt tacgggaccg cagaagaatt cacgggggtg tggctctccg atcgtccgct
     1261 ggacgtgaat gagggcgcag agggcgacac agcactgatg attagcttct caatcccgct
     1321 ctctgcgcta gctaactatg aatggattga ggagggcaaa ggctatcgag aatggctggt
     1381 gcctgctgaa atcatagggc gacacgctac cgtttcaatt gtggggccgc ggcatcgcat
     1441 tcctaaactc aggccaattg cctgatccaa tgaccccata gaaagctcgc ccacgcattc
     1501 ttcggagaag agcagcttta aggcaccggc gcctcgatgg tgacgaagcc ctcccgctgc
     1561 aagcattcga gcacagcgag cgtcgtcgcc aagggctccc cgcttttgct gtagtgccgg
     1621 cgcccgtgcg cgatcgcgat ctcgacgagg gcggccagga actcggccgt cgcccgcctg
     1681 tcattgatgg tcatgcccgc agccatttca acttttcagc tctggcttcg cgcctggcgc
     1741 gacgaaattc gtcgagaaag cgcatgaccc gctccacatc ctcgtcgacc ttatgcttcg
     1801 ccggcagcac cgccgcgatt tttgcagcag gggcaggcaa ggcaggcaaa acaccagcga
     1861 cccattccac aaagccctca gcggcggcgc gatgaacaag gttgcccccc tcacaaattc
     1921 tgcggtcgcg ttcgttcaga tacagaccga tgatgttgtc ggcgtgtttg cgcgaccagc
     1981 caaattcgat tttaatccat gccgaccacg cccgccgaac cttgaacagt ttacggcata
     2041 aaacgagccg ccggccaatt tcaatttcaa ccgcgccctc gaagctctgg cagtcaatcc
     2101 gtcgaattgc gtcagcatgc tcgtcaagct ccacgttcgc cgggtaaaga tcggaccgaa
     2161 gagcttttcg cgggatgccg gtcacggcct caacttggag aacgcggccg acgggaacgc
     2221 aaaaccagtc ccgtactgcc ttgtcgctga tgcccagcgc acgcgccaag gccccaacac
     2281 taccaactgc ctcgatggct gcaattagtc ccgcgtcccg acggcgcatg atcacccctt
     2341 cgatgattat gccgcgcgtg agcggctctc tcgagtctcc ggccaaggat ggctgcgaga
     2401 gagccgcgat caccgcgcga tgaaacgtca gattttttgc aagccttggc cggaggacct
     2461 ctgcataatc tattaaccga agcgcatgca atagggagac actagtgtct cccggtcgcg
     2521 ctaccggagg ccaaacctac gccgccggcg gcacgccgtc gggcacctcg ggcgtgtgtt
     2581 gcgaacgccg aggagggccg ggacggatca aaccgaatcc cgccccggcg cgggtcaact
     2641 caatcgcgtc cgcgagctgt cgcacgcaga gccggaaacg gagaatctcg gcggcgaggg
     2701 cgtgcatcgt cgcttcggga ttcccggcgt cgaattctgt gataaccatc acacgttgcc
     2761 gccgctcctc ctcgtcgtgt cggcgccgat gctcggccca ctgctccgcc catcgctccg
     2821 ccgctcgctt cctggccatg gtgctctaat cctctgagct ccaccctacc aatcctccgc
     2881 cgcctgaagg atcgccacca tgctccagta ccacgacttg ctggaacgga ttctcgccga
     2941 cggcgtcgaa aagcacgatc gcaccggcac cggcacggtg tccgtgttcg ggcatcagat
     3001 gcgcttcgac ctcgcgcgcg ggttcccgct caccaccacc aagaagctac acttcaaatc
     3061 gatcgcccac gagttgctct ggttcctcgc cggcgacacc aacgtcgaat atctccacga
     3121 gcatagcgtc cacatttggg ataagtgggc aaacgcgaac ggcgagctcg ggccgatcta
     3181 cggccggcaa tggcgcgcct ggccgacgcc cgacggcggc gcgatcgatc agatcaccaa
     3241 ggtggtgtcc gccatccgtc gcaatccgga ctcgcgccgc cacatcggca ccgcctggaa
     3301 cccggccgag gtcgacaaga tggcggtgcc gccgtgccac tgcctgttcc agttctacgt
     3361 cgccaacggt cggctttcat gtcagctgta tcagcgctcg gccgacgtgt tcattggcgt
     3421 gccattcaac atcgcctcct acgcattgct gacgctgatg atggcgcagg tagccggact
     3481 caaacccggc gaattcgtgc acactctcgg cgacgcccac ctttatcaca atcatctaga
     3541 gcaggcgcaa ctgcaactct cacgcccgcc acggcagttg cccacggtga ccctcaatcc
     3601 ggccgtgacc gatctcttgg ccttccgctt caaggacttc tcgctccgag actacgatcc
     3661 tcacccgcac atcgaagccg aggtggcggt gtgagcgggc gcaaatcaag tgctggagtc
     3721 aagcacgtgt gctaggggca aagtcgctca taaaacacgc gacggggccc cgattcatat
     3781 tcgccggctt cagaaccggg gcatggggcc gtaccgttgc cgcctttgcg gaaattggca
     3841 catcgcgaat agccggaagg attggaagat ccaggccagg attgatcatt tactgcagcg
     3901 ctgagccgat cgctcatcgg cgcgcgccgc cgccgcgccg cggtgattcc gctgcgtggc
     3961 tgaggttcac cgcacttgcc gcgcgcggac tcgaagcggt caatcgacat gcgcaaatca
     4021 tggcgcaacg cgcgcaggag cgcaatctga caacccccca catggcgagg ctcgcatgcc
     4081 gaaatttcga gcggactacg atgtgaaggg cagtcacgtt ttgccgcccg acagcaaacc
     4141 catcatcctg aggggcggcg cgccaccttt tgagatgaaa tttcgcaatg ccgatcccga
     4201 caagtccggg catgcccccc atctaattgt cgaagtgatc gcagactccg cttcgatcga
     4261 tgacgtcgct aacgagttca gatcgctact ggccgcgcag ttggacattc tcagcttcgt
     4321 cacgcaatcg accttcgtca tcgatcaatg tcggcgcgtg gtggaatggg agccgttcca
     4381 aaaaacgaga gagtatcggc ctgtccgaag gtttgatccg ctctacccgc ccagtccaga
     4441 cttgcgtcag ttcctgttcg acgcggccca gattatcctg caggccaaac ctccagatta
     4501 cgtgctccag gcgctccact actttcgtct cgggctgttc gagccgcaac ctgaggatca
     4561 gtttatgcat ttttggcggg ctatcgaaac gatcgcagag ggcgcgaagg agaccacacg
     4621 aattccgatc ccttgcccaa aatgtagtgg tgagctgttc tgcgcaattg caacgaggtg
     4681 cccagacgaa ggccgatatc gcggcaagcg attcgcgagt tgctcgagcg aatcttcccg
     4741 acttcgccta gtctatatgc aactctcgtg gggacacgtg accacctgtt ccacggccgg
     4801 gcaccgcact tagtggagac agagatcggg cgccctctcg gcgcggtcgt caacgatgca
     4861 ggggtgacgg catggtccgt catccagcat tctatcccgc cactcggaac gcaaattaaa
     4921 gtcatgcagc gcggcgggcc cttcgcgaac tacatgctga ccgcatcgcc agtgatgaca
     4981 ttcacctact cgaccgacgc gccgcatccc accgaggacg aaatgcccaa ggtggaagta
     5041 aacttggtca cgacgttcac agatcaggcg tagctcaaag ccgccgcttg gcggcgcttt
     5101 cgacgaagcg cttcgaggca ctgacgatag gcttcccagc caggggacga tcgccgtatc
     5161 gtccccctaa acacccaacc ccaccgcagg agtctttccg cctctcgagc acagctccga
     5221 aatttggccc gccggagcga aaccgcgaaa aagtagtgct gcggtgccga tttgaaaaac
     5281 cgcgagccaa caggaaggtc actggtgagc gaaacgccga gggtccgaac cagatgacct
     5341 gaccaaattg cccctcaccg tttcgcatcc ctgcggtccg aatcggtgac aaggtcgtcg
     5401 agctcagcct gcgacgcctc ctctttgact cgattcttgg ctcgcggccg cttcgcttcg
     5461 cgctcgcgtc cctcgacaaa tctcgtccac gcttggcgct tgctgtccat gctcatgtct
     5521 gccaccgcta ctcgatagcc tcgatcgcat tccttgcgag ctcgacgcgc tgctctccac
     5581 caagcaaacg caaaagcacg agtacacgtt catgggggcg cagaatcgcg agcatcccaa
     5641 tctgattttg cagcggaccg ctaaccacct tcacccgcat acctggtacg aatccgcgcg
     5701 gccgcggcaa ctcgacaaag ccgttgcgtt cgcgggatct gatttcggcg atgacagcat
     5761 cgggcacgcg cgccggctgc tcaccatcca tcaccagacg gcgcacaccc ggactccacc
     5821 ttgcgctcca ccaagccgaa acaactaata cgaaggcgta accgggaaat agcggcgggg
     5881 tgcgcacgat cttgcggcca tgacggcgaa gctgctcccg aatccggggg caataaattt
     5941 cgaagccgtt gaggccgagg cagtgcaccg ccaagcgttc acgctgtggc tcgagctgcg
     6001 cacacgccca atacatggcg ccctcctatc gcgcacgctt gcgataacgg acatggcgga
     6061 gatcgccgcc gtactcgatt ggcggggcgc tcttgagctt ggtgggccga agttcttgtt
     6121 cgatgtccca atgcgcacgg ccgtcagtgg gcggatcgaa gaaggcgcgc tgcagagatg
     6181 ctaccgcccg gtagacggcg ccttcacccc agcatgggac gcgggtgaga gcatcctcgg
     6241 cggccgcccg gaaggcggcg cgatcaggcg gtgataatgg actggcaaga cgggaaatca
     6301 gctgctcgac atcgtcgggg atggtggaca tgagacggcc tcgcgtggga taaactggcc
     6361 gccatcaagg tctctttgtc ttgcttcagg agcactcggc ccgctgcccg gcgggccgct
     6421 ctttgtagga cggacggcga ttcccacgca actgatggcc tctgctcctg tagccccggc
     6481 cccgacctgc tgtggctgag gcgcgctcgg gccgcggcta ttcccgattc ctgctgatta
     6541 gtaggggctg attggcagaa tgacagattc gcagaatggt gaggtttctg cgacgccatg
     6601 gttgtagtca agggcaagcg ctgaccctcg gaggcgttgc agaaaccttc gttgcactga
     6661 accgcgggcc acgagttcag actatggaga tttggtcgcg tggccgc
//
LOCUS       DATLJT010001060         3008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3060690,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001060 DATLJT010000000
VERSION     DATLJT010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060690"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VM910_24340"
     CDS             complement(<1..302)
                     /locus_tag="VM910_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HVI65685.1"
                     /translation="MADVLIVIPARMASSRLPGKPLMDIAGEPMIVHVMRRAQAARLG
                     EVVVATDSDVVAACVEKAGGRAVMTRADHVSGSDRIFEALDVADPQRRSAIIVNVQG"
     gene            529..1098
                     /locus_tag="VM910_24345"
     CDS             529..1098
                     /locus_tag="VM910_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HVI65686.1"
                     /translation="MDSFEVNKILGAILATCLGVLSINIAAGAIFAPVKPAKPGYEIG
                     VPEQAPAGKPAEQEKQESIEQLLAKADVARGENAVKKCQACHTFTKGGRNLIGPNLWG
                     VIGRPKASEAGFNYSAALKAKGGNWTIDDLNQFINNPRGYIPGTNMTFAGIQRGSERA
                     DVIAYLNSLSDNPAPLPKAAEAAGSPKAQ"
     gene            1323..>3008
                     /locus_tag="VM910_24350"
     CDS             1323..>3008
                     /locus_tag="VM910_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI65687.1"
                     /translation="MRLTRRSVVRTGVLAALSPILDRFGAPFLGASARAQEHALDWKH
                     GLSLFGELKYPAGFKHFDYVNPNAPKGGSVRLMALGTFDNFNEVVAGLKGSIAAGAGI
                     LSDTLLAPSLDEVSTDYGLIAEAVTHPSDFSSATFRLRANARHHDGKPVTVEDVIFSM
                     QAFKKHSPMLAAYYRHVTKMEQTGEREVIFIFDAPGNREMPLILGQLNVLPKHWWEGT
                     DAAGRKRDVGATTLEPPLGNGAYRIKEFVAGRTVVYERVKDYWGKDLNVNIGRDNFDE
                     LRYEYFRDATVAIEAFKADHLDWRTENVAKNWATAYEFPAVKEKRVVLEEFPQRNRGI
                     MQAFAFNTRRDKFKDARLRRAFNFAYDFEEMNKQIFFGQYTRINSYFDGTELASSGLP
                     QGQELEILETVRDKVPPEVFTTAYTNPVGGNSENVRANLREAMRLLKEAGYEIRNQKL
                     VNPKTGEAMSVEILTEDPSIERIILFYKPSLERLGITVNVRTVDDPQYENRLRNWDFD
                     IIVASWPESLSPGNEQRDYWGSPAADTPGSRNYVGIKNPAVDALIERVIFAKDR"
BASE COUNT          609 a          986 c          911 g          502 t
ORIGIN      
        1 ccttgcacat tgacgatgat ggcgctgcgc cgctgcgggt cggcgacgtc caaggcctcg
       61 aaaatgcgat cggatcccga gacgtgatcg gcgcgcgtca tcaccgcgcg cccgccggct
      121 ttctcgacgc aggccgcgac cacatccgaa tcggtcgcca ccaccacctc cccgagccgc
      181 gcggcctgcg ctcggcgcat gacgtggacg atcatcggct cgcccgcaat gtccataagc
      241 ggcttgccgg gaagcctgct cgacgccata cgcgcgggga tgacaatgag aacgtcggcc
      301 atcgctgcgc ctcggccggc tccggcccgc tggaaaaacc tacggtttgc ctccccctga
      361 ggagagggcc ctaaagcggg tttacgcacc tcctcgggaa gaggatgacg cgtcgattgg
      421 ccgcttatac gggttgccag agccgaggca aaccggctat ttgtgcaccg ccgcaatctg
      481 ccgcggccgc ttccaattcc cccgaccttc cgcgcgagcc ttgaggcgat ggactccttt
      541 gaagtgaaca aaatcctcgg tgccatcctg gccacctgcc tcggcgtgct ctcgatcaac
      601 atcgccgccg gcgccatctt cgcgcccgtg aagccggcca agcccggcta cgagatcggg
      661 gtgccggaac aggcgccggc gggcaaaccc gccgagcagg aaaagcagga gtcgatcgag
      721 cagctgctcg ccaaggccga cgtcgcccgc ggcgagaacg cggtgaagaa atgccaggcc
      781 tgccacacct tcaccaaggg cgggcgcaac ctgatcggcc ccaatctgtg gggcgtgatc
      841 ggccgtccga aggcgtcgga ggcggggttc aactattccg ccgcgctcaa ggcgaaaggc
      901 ggcaattgga ccatcgacga cctcaaccag ttcatcaaca atccgcgcgg ctatattccc
      961 ggcaccaaca tgaccttcgc cggcatccag cgtggcagcg agcgcgccga cgtcatcgcc
     1021 tatctcaact cgctctccga caacccggca ccgctgccca aggcggccga ggccgccggc
     1081 agcccaaagg cgcaatgaag gagccccggt ccgcggtcgc ggccccttat cgcttacttt
     1141 aagatttggt aatgaacgcg ttgcagccca ccacatcagt ggcgcaggcg cggtcgcggc
     1201 ctacttataa agtgatggca aagtgatggt ccgaccgcgc acatgtccgt gcccagccac
     1261 ccaaaatagg acataatccg gccagtgcgt gcgtcattga cgtcgttccc gcggaggttt
     1321 ccttgcgcct gacccgtcga tccgtcgttc gcaccggtgt gctcgcagct ttatcaccga
     1381 tcctcgatcg atttggagcc ccgtttctcg gcgcctcggc gcgcgcgcaa gaacatgcgc
     1441 tcgactggaa gcatggattg tcgctgttcg gagagctcaa atatccggca ggcttcaagc
     1501 atttcgacta cgtcaacccg aacgcgccca agggtgggag cgtgcgcctg atggcgttgg
     1561 gcacgttcga caacttcaac gaggtcgtcg ccgggttgaa gggatcgatc gcggcgggag
     1621 cgggcatcct ctccgacacg ctgctggcgc catcgctcga cgaagtatcg accgactacg
     1681 ggctcattgc cgaagcggtc acccatccga gcgattttag ctcggcgacc ttccgcttgc
     1741 gcgccaacgc gcgccatcat gacggcaagc cggtgacggt cgaggacgtg atcttctcca
     1801 tgcaagcgtt caagaagcac agcccgatgc tggcggccta ttatcggcat gtcacgaaaa
     1861 tggaacaaac tggagagcgc gaggtcatct tcatcttcga cgccccgggt aatcgtgaaa
     1921 tgccgctgat cctgggtcag ctcaacgtac tgccgaagca ttggtgggag gggaccgatg
     1981 ccgccggcag gaagcgcgat gtcggcgcca ccacgctcga accgccgctc ggcaacggcg
     2041 cctaccgcat caaggagttc gtcgccggcc gcaccgtcgt ctacgagcgg gtgaaggact
     2101 attggggcaa ggatctcaac gtcaacatcg gacgcgataa ttttgacgag ctgcggtacg
     2161 aatatttccg cgacgccacg gtcgccatcg aggccttcaa agccgaccat ctcgactggc
     2221 gcacggagaa cgtcgccaag aactgggcga cggcctacga gttcccggcg gtcaaggaaa
     2281 agcgcgtcgt gctcgaggaa ttcccccaac gcaatcgggg gatcatgcag gctttcgcct
     2341 tcaatacccg gcgcgacaaa ttcaaggacg cgcggctgcg gcgcgcgttc aatttcgcct
     2401 atgatttcga ggagatgaac aagcagatct tcttcgggca atacacgcgc atcaacagtt
     2461 acttcgacgg caccgagctc gcctcgtccg gtctgccgca gggccaggaa ctggagatcc
     2521 tggaaacggt gcgggacaaa gtgccgccgg aagtgttcac caccgcgtac acaaatccgg
     2581 tgggcggaaa ctccgagaac gtgcgggcca atctgcgcga agcgatgcgg ctgttgaagg
     2641 aggccggata cgaaatccgc aatcagaagc tggtcaaccc caagaccggc gaagccatga
     2701 gcgtcgaaat cctcaccgag gacccgagca tcgagcgcat catcctgttc tacaagccct
     2761 cgctcgagcg gctcggtatc accgtgaacg tgcgcacggt cgatgatccg caatacgaga
     2821 accggctgcg caactgggat ttcgacatca tcgtcgcgtc gtggcccgaa tcgctttcgc
     2881 ccggcaacga gcagcgcgac tactggggct cgccggccgc cgacacgccc ggctcgcgca
     2941 attacgtcgg catcaaaaat ccggcggtcg acgcgctgat cgagcgggtg atcttcgcca
     3001 aggaccgc
//
LOCUS       DATLJT010001061         1522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2831473,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001061 DATLJT010000000
VERSION     DATLJT010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831473"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VM910_24355"
     CDS             <1..313
                     /locus_tag="VM910_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65688.1"
                     /translation="AYGDPQAILPPPEYDYYPPGGFRVFTVPTHSELVRMCGQEHAPF
                     PSCGGRDKNGVCIILALPRLEMPAMGYDYDDVKRHEFGHCNGWPKDHAGMRSRLSPGP
                     I"
     gene            complement(795..1130)
                     /locus_tag="VM910_24360"
     CDS             complement(795..1130)
                     /locus_tag="VM910_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65689.1"
                     /translation="MGVVRMLPMWVLALPIWLVVFIVLYALATLYVAIKYREVRKFLA
                     GAFFVSSGACWYLWMTGVSFPIVLPYAGTVSVETPEISGQRAIVHFILFLLCFYFGFI
                     SKPKQKLSN"
     gene            complement(1269..>1522)
                     /locus_tag="VM910_24365"
     CDS             complement(1269..>1522)
                     /locus_tag="VM910_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65690.1"
                     /translation="PETKTPMSWLRMIAVVLLVVGIPAAEAAPLVPSSDLPGRERYRF
                     TPSPLDRFMQPNPPARPLLHCGPRGGWWGKARSRRTRPC"
BASE COUNT          360 a          454 c          464 g          244 t
ORIGIN      
        1 ggcctacggc gacccgcaag ctattttgcc gccgccggaa tatgactatt acccgccagg
       61 gggattccgc gtattcaccg tgccgacgca ctccgagtta gtgcggatgt gcggccagga
      121 gcatgcgcca tttccctctt gcgggggaag ggacaaaaac ggagtctgca tcatcctcgc
      181 gctaccgcga ctcgaaatgc cggctatggg ttacgactac gacgacgtga agcgccacga
      241 atttggccac tgcaatggct ggccgaaaga tcatgctggc atgcgctcgc gactgtcccc
      301 aggacccata taaggaagca gaggaggcgt ggaaaaagac gccagagggc acccaaacgg
      361 aaaaagaaat aacccgatgg cgtcagctgg aagaggagca atggcaaaag tccaggcaag
      421 ggacgaagaa atgaagggtg ccacgagcga ctagtgttgc ggcgctgctg gcaacaagga
      481 cagccgcacg cttggcgaag attcagagaa aggcgcgacc ggcgaacgcc tcccgcgccc
      541 gacgaacaca ataaggctgg agatcagatt gggcatgcgc cgcaccctgc ccgcatcacg
      601 ggcggcgagg agggtgtcgc cgcctcctca ccgcccccgc gtcagtcgcc gtcgtctgtc
      661 agttcggccc gacacggatc tccttaaact aagccgtgta ctcataaatc gtggtgccct
      721 gatgtccgct atgcccctga tagcggcgcg aaagcggaca tcgccggagg tccgagttgt
      781 gccagaagcg gcccttagtt cgaaagcttt tgcttcggtt tgctgatgaa accgaaatag
      841 aagcacagca agaatagaat gaaatgtacg atggcccgtt ggccgctgat ctcaggtgtc
      901 tcgacagaaa cggtcccagc gtatggcaac acaatcggaa acgatacgcc ggtcatccac
      961 agataccaac acgctcccga gctaacaaag aatgcgcctg cgagaaattt tcgcacctcg
     1021 cgatacttga tcgcgacata gagagttgcg agagcataga gaacaatgaa gacgacgagc
     1081 cagatcggca acgccaagac ccacatcggt aacatacgga ccacacccat ttatgagtac
     1141 acgccctaga ttcgtcggcg cggggtagct cacgatgcat tcggcgtccg tcgagagata
     1201 gcctcggcgg cactcgaaga ggatccgacc ggggccgtct gcagcgagaa cgccgcaagc
     1261 gtcgcccctc aacagggtcg cgttcgccgc gatcgcgcct ttccccacca tccgccgcgc
     1321 ggaccgcagt gcaagagcgg cctcgccggc gggttcggtt gcatgaaacg gtcgagcggc
     1381 gacggcgtga agcgatagcg ttcgcggccg ggcagatcgc tcgacggcac gagcggcgct
     1441 gcttcggctg cgggaatgcc cacgaccagg agcaccacgg cgatcatgcg cagccagctc
     1501 attggcgttt tcgtctcagg gg
//
LOCUS       DATLJT010001062         3535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1912500,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001062 DATLJT010000000
VERSION     DATLJT010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912500"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VM910_24370"
     CDS             complement(<1..720)
                     /locus_tag="VM910_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65691.1"
                     /translation="MTGANSGIGKATAIALGEAGADVVVNYVSGEDHADAVVGEIRRS
                     GAKAYAHKADVSQEEQVQQMFRRMLEELGTIDILVNNAGLQRDAPFHEMTLAQWNTVI
                     GVNLTGQFLCAREAVRHFLQRDVVSRVSCAAGKIIFMSSVHETIPWGGHTNYAASKGG
                     VMLLMKSLAQELAPRRIRVNSIAPGAIRTPINTAAWETKEAYDQLMTLIPYKRIGEPI
                     DIARAAVWLASDDSDYVNGASL"
     gene            complement(848..2776)
                     /locus_tag="VM910_24375"
     CDS             complement(848..2776)
                     /locus_tag="VM910_24375"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVI65692.1"
                     /translation="MPIRDYALIGDCHGCALVSRSGSIDWCAFGRFDAPPSFCRLLDA
                     EKGGFLSTTPADAFEAQRAYLDRTNILRTVLTTATGKVAITDYMPVGRSPAAAAHDYV
                     TLAAPGLLIRTIDGLAGSVALDVAFRPSLDYARAVPRLFTTSHGVFAENGPALYSPLD
                     LSIDGDCARGRLQVKAGARQHLVVASAALARPPSPETIERLLAITRAFWIEWIGYCRY
                     DGPYRDIVMRSALALKLLTYAPTGAIVAAATTSLPEEIGGERNWDYRYCWLRDASFTL
                     LALAALGYSGEAKAFGRFMKLVCSKRPAVIQIMYGIGAEMALDEQILEHLEGYCGSRP
                     VRIGNGAYNQRQLDVYGEFADWAYLVTALGGKFDAEDRSLIKRLVSFAAEHLNEPGQG
                     LWEARGAPRHHVYGKVMAWVALDRGLRLLGNVPEWSTQRDQLQAEILRRGRDAVGGHL
                     LQAYEHQGTDAALLLIPMLGFPIERTTLERSVAAIERELRRGDYIERYHTEDGLAGAE
                     GAFLICSFWLVDAYLALGREKEARELFERLVGRANDVGLFSEEIDPVNDAFLGNFPQA
                     FTHLALIGNAIHLDMYGKGGPDAIAGTHADRARRAVGAIFGWRAILEACKAAGRVGRI
                     WSSRASILPAALARQLSA"
     gene            3350..>3535
                     /locus_tag="VM910_24380"
     CDS             3350..>3535
                     /locus_tag="VM910_24380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65693.1"
                     /translation="MSRESFELADFFRALPRAIVGALIGTIVFTLMMKFLAPMMIGHP
                     MDIAAVLGTFTGLGTSAG"
BASE COUNT          669 a         1071 c         1118 g          677 t
ORIGIN      
        1 cagcgaggcg ccattgacgt agtcggaatc gtcggaagcg agccagaccg ccgcgcgcgc
       61 aatgtcgatg ggctcgccga ttcgcttgta cggaatgagc gtcatcagct ggtcataggc
      121 ttctttggtc tcccaggctg cggtattgat tggtgtccgg atagcccccg gcgcgatgct
      181 gttgacgcga atgcgacgag gagcaagctc ctgggcgagg cttttcatca gcagcatgac
      241 gccgcctttt gatgccgcgt aattggtgtg cccgccccac ggaatggttt cgtgcactga
      301 gctcatgaaa atgatcttgc cggcggcgca ggaaacccga gagacgacgt cgcgctgcag
      361 aaagtggcgt acggcctcgc gagcgcacag gaactggccc gtcaagttga cgccgatgac
      421 cgtgttccac tgcgcaagag tcatttcatg gaatggggcg tcgcgctgaa gcccggcatt
      481 gttgacgaga atgtcaatcg tcccgagttc ctccagcatg cggcggaaca tctgctgtac
      541 ttgctcttcc tgagagacgt ccgccttgtg cgcgtatgct ttcgctccgg atcgacggat
      601 ctcgcccacc accgcgtccg cgtgatcctc gccgctgaca taattcacca ccacatcggc
      661 gccggcctcc cccagcgcaa ttgcggtggc cttaccgatg cctgaatttg cgccggtcac
      721 gagggccttc tggccgagca gaaccttcgg gatcgcacgg gaaggtgttt tcgaggcatc
      781 tgatgtaggg gccgatgaag cagacatgac ttttcctctc gaaccagttt gacgcccgca
      841 tccgcagcta cgcgctcagt tgccgggcga gagcggcggg caggatcgac gcgcgcgagg
      901 accaaatacg gccgacgcgt ccggcggcct tgcaagcttc caatatcgcg cgccagccga
      961 agatcgcgcc cacggcccgt cgcgcgcggt cggcgtgggt tccggcgatc gcgtccgggc
     1021 cgcccttccc gtacatatca aggtggatgg cgttgccgat cagcgcgagg tgagtgaagg
     1081 cctgggggaa attgccaagg aacgcgtcgt tcaccgggtc gatttcctcg gagaagagtc
     1141 ccacatcatt ggctctgccg accaggcgtt cgaacagctc ccgagcctct ttttcccgtc
     1201 ccagagcgag gtacgcatcc accagccaga aactgcagat gagaaaggct ccttccgcgc
     1261 ctgcgagccc gtcttccgtg tggtagcgct cgatatagtc gccccggcga agctcgcgct
     1321 cgatggcagc aacggaccgc tcgagcgtcg tccgctcaat cggaaacccg agcataggga
     1381 tgagcaggag ggcggcatcg gtgccctggt gttcataggc ttgcaacaga tgaccgccca
     1441 cagcgtccct gccgcggcgc aggatttcgg cctgcagctg atcgcgttgc gtcgaccatt
     1501 ccggcacgtt gccgagcaag cggaggccgc ggtcgagcgc aacccaggcc attaccttgc
     1561 cgtagacatg gtggcgtggg gcgcctcgtg cttcccacag gccctggccc ggctcgttga
     1621 gatgttcggc cgcgaacgac accagccgtt tgatcagaga gcgatcctcc gcgtcgaact
     1681 tgcctcccaa cgccgtcacc agatacgccc agtcggcaaa ttcgccgtag acatcgagct
     1741 ggcgctgatt ataggcaccg ttgccgatcc gcaccggtcg gctgccgcaa tacccctcga
     1801 gatgctcgag aatttgctcg tcgagcgcca tttccgcgcc gatgccatac atgatctgga
     1861 ttacggccgg ccgcttgctg cagaccagct tcataaatcg gccgaatgct ttcgcctcgc
     1921 cgctgtaacc gagcgccgcg agggcgagga gtgtgaagct cgcgtcgcgg agccagcaat
     1981 atcgataatc ccagttgcgc tcgccgccga tctcctccgg gagcgaggtg gtggccgcgg
     2041 ccacgatcgc cccggtcgga gcataggtca gcagcttcag cgcgagggcg ctgcgcatga
     2101 caatgtcgcg gtatggtccg tcataccggc aatagccgat ccactcgatc cagaatgccc
     2161 gggtgattgc caacaggcgc tcgatggttt ccggtgaagg cggccgtgca agcgcggcgg
     2221 aagccaccac gagatgctgg cgtgctcccg ctttgacctg aagccggccg cgcgcgcaat
     2281 cgccgtcgat cgagaggtcc aaaggcgaat aaagcgccgg cccgttctcg gcgaagacgc
     2341 cgtgtgacgt cgtgaacaag cgtggcaccg cgcgggcata atcgagcgag gggcggaagg
     2401 cgacatcgag cgccacgctt cccgcgaggc cgtcgattgt gcggatcagc aaaccgggcg
     2461 cggcaagcgt cacataatcg tgcgcggctg cagcggggct acggccaacc ggcatatagt
     2521 ccgtgatcgc gaccttgccg gtcgcggtgg tcaatacggt gcgcagaatg ttggttcgat
     2581 cgaggtaggc tcgctgtgcc tcaaaggcat ccgccggcgt tgtcgagaga aagccgcctt
     2641 tctccgcgtc gagaagccgg cagaatgaag ggggagcgtc gaagcgccca aaggcgcacc
     2701 agtcgatgct gcccgagcgg gacaccagcg cgcagccatg acaatcgccg atgagcgcgt
     2761 agtcgcggat cggcaaatac ttgggcggtt tccgcgaccc aggatcggct tgcgatcggc
     2821 gcggttcttg tggggcggac gcggggttca cggcgaaaac cgcactttgt tccaccagtt
     2881 acctagcagt gcggcggcat accttttcat cgctcaagat gagagcccga cgtttccatg
     2941 ccaaaaacgt cggggtggtg cgggaccaag tgatggcttc tgccgtcgcc gaccattccg
     3001 gcgctgcacg ctcaccgtgt tgagcgggga taggttagtg agggtcgttg acgaactgaa
     3061 ttacttatta ggtatcaatt cgatgttgga tagctattaa cgtcgccccg gtggtggatg
     3121 gtgcctcgtt taatacgaga cgcgcatgaa ttcgatgcga gaacagcatt tccgtcgcaa
     3181 ttgcgcgcag cacataatac cggtcgcatc tgttcggcga aatgtcggac agagaagaac
     3241 gctgaattgc ctaacttcgg gagcgcgtcc acggttggtt tattgaatag cgtcctggct
     3301 ttcgccgccg gcaggctttt ttcaaatcgt ggcaagagga ggaacgccga tgtctcgaga
     3361 atcgttcgaa ctagccgact ttttccgcgc cctgccgcgc gccatcgtcg gggctttgat
     3421 cgggaccatc gtcttcacgc tgatgatgaa attcctggcg ccgatgatga tcggacatcc
     3481 gatggatatc gcagccgtgc tcggaacatt cactggatta ggcacttcgg ccggg
//
LOCUS       DATLJT010001063         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_304386,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001063 DATLJT010000000
VERSION     DATLJT010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304386"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VM910_24385"
     CDS             complement(<1..415)
                     /locus_tag="VM910_24385"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65694.1"
                     /translation="MPRWTTGLLAALLLAGAGSAYAQTYPSATVKLIVPVPAGGVTDT
                     MARIVAQRLTEAWGQPVVVDNRPGGNYAVGAQAVAKSPPDGLTLLVAPDSTVTANPHL
                     FSKLPYDPVKDLTPIIVLCRITPVLVINPSLAVQSV"
     gene            671..1252
                     /locus_tag="VM910_24390"
     CDS             671..1252
                     /locus_tag="VM910_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI65695.1"
                     /translation="MAAERRAPTAKDNSDEALIAKIASGSRLAMQVLFARHHARIYRF
                     VLRLLGSEALAEDITSEVFLGVWRQAHRFEARSAVTTWLLAIARYKALAELRRRPEAC
                     DEATADASDPADDPEATFEIKHRGEILRNCLTRLSPRHREIIDLVYYHEKSVQEVAHI
                     LGVPGNTVKTRMFHARKKLSALLEARGVVGALS"
     gene            complement(1271..2005)
                     /locus_tag="VM910_24395"
     CDS             complement(1271..2005)
                     /locus_tag="VM910_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVI65696.1"
                     /translation="MSIPGGKRPAERTRDKLALLRNHSLFRDLPPAVIEHLGSYMKTR
                     RVARGNTIFAKGDPGTGLMGVLAGTVKVSVASAEGKDIVLNLFHEGEVFGEIALLDGR
                     PRTADAVAMSDCELVVIERRDFVPFLSSHPDVMLKFIEILCSRLRRTSEQVQDITFLN
                     LPTRLAKTLLQLTAAEDGSATTRKAAITQREISQMIGISRESTNKQLRAWAKRGWIRL
                     ERGGVNVVAPDKLAAIATEGSEFDRS"
     gene            2350..>2657
                     /locus_tag="VM910_24400"
     CDS             2350..>2657
                     /locus_tag="VM910_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI65697.1"
                     /translation="MQMHQIRYFLALCEERNFTRAAGRCGVSQPSLTNAIIALERELG
                     GALFQRKPLIALTALGRAIQPYLDRIAQNAAHAREAAQALADVPPANSEPAPREEIAG
                     "
BASE COUNT          501 a          855 c          845 g          456 t
ORIGIN      
        1 ccacgctctg caccgcaagc gacgggttga tcaccagcac cggcgtgatg cggcacaaca
       61 cgatgatggg cgtgaggtcc ttgaccggat cgtaaggaag cttgctgaag agatgcggat
      121 tggcggtgac ggtggagtcc ggcgcgacca ggagcgtgag cccatccggc ggcgatttcg
      181 ccaccgcttg cgcgccgacg gcataattgc cgcctggacg gttatcgacg acgaccggtt
      241 gcccccacgc ctcggtcagt cgctgggcca cgatacgggc catggtgtcg gtcacgccgc
      301 cggccgggac cgggacgatg agcttgacgg tggcgcttgg ataggtttgc gcgtacgcgc
      361 tgcccgctcc cgcgagcagc aacgcggcga gcagcccggt cgtccacctc ggcatcgtcc
      421 ctccctatcg atttgcgcgg cggcgatttt ttgctgttgg ttcagcacgc aagcggaaaa
      481 tagaccagca cccaggcccc gccaacctgg cgagccgggc aagccgtcgc ggtcaaacgc
      541 gggcctgaaa gctggggtcg gctgtgtgaa gtagttaaca aaaggtttcc gcggggcagt
      601 ctaggcatat tggtagccca aaacagggga gctaccaatg cgcaacgact ttgccgagct
      661 cgtcccgggt attgccgccg agcggcgggc gcccacggct aaggacaact cggacgaggc
      721 gctgatcgca aagatcgcat ccggtagcag gcttgcgatg caggtcctgt tcgctcggca
      781 tcatgcgcgc atctaccgtt tcgtattgcg cctgctcggc agcgaggcgc tggcggagga
      841 catcaccagc gaagtgttcc tcggggtctg gcggcaagcc caccggttcg aggcacgctc
      901 ggcggtcacg acctggctgt tggccatcgc ccgttacaag gcgctggccg agctgcggcg
      961 ccggcccgag gcgtgcgacg aagcgaccgc ggacgccagc gacccggcgg acgatccgga
     1021 agcgaccttc gagatcaagc atcggggaga aattttgcgc aattgcctca cccgcctgtc
     1081 cccgcgccat cgcgagatca tcgacctcgt ctactaccat gaaaaatcgg tgcaggaggt
     1141 ggcgcacatc ctcggcgttc cgggcaacac cgtcaaaacc cggatgttcc acgcacgcaa
     1201 aaagctctcg gcgctgctcg aagcgcgagg cgtcgtggga gcgctgtcat agcgacgtac
     1261 gaatctaagc tcacgatcga tcgaattccg aaccctcggt ggcgatggca gcgagcttgt
     1321 ccggcgcaac cacgttgacg cccccgcgtt ccaggcgaat ccaaccgcgc ttggcccatg
     1381 cccgcagttg tttgttggtg ctctcgcgag atatgccgat catttgactg atctcgcgtt
     1441 gcgtgatcgc cgctttgcgt gtggttgccg aaccgtcttc ggccgcggtc agctggagca
     1501 gggtcttcgc caagcgggtc ggcaaattca ggaatgtgat gtcctgcacc tgctcgctgg
     1561 tccggcgcag ccgcgagcag aggatctcga tgaacttgag catcacgtcg gggtgactgc
     1621 tcagaaacgg aacgaagtcg cgccgctcta tgacgacgag ctcgcagtcg gacatggcga
     1681 cggcatcggc ggtgcgcggc cgaccgtcga gcaaggcgat ctctccaaaa acctcgccct
     1741 cgtgaaagag gttgagcacg atgtctttgc cctcggcgga cgcgacgctg accttgaccg
     1801 tcccggcgag cacccccatc agcccggtgc cgggatctcc tttcgcgaag atggtgtttc
     1861 cgcgcgccac ccgacgcgtc ttcatgtacg agccgagatg ctcgatcacc gccgggggaa
     1921 ggtcgcgaaa cagcgagtga ttgcgcagca aagccagctt gtcacgcgtg cgttccgccg
     1981 gccgcttgcc gcctggaatc gacatgagag ctcccccgtc caaacgaatc ttgcaattcg
     2041 acctgcgctc tcgaacacgc ccgcgacaac cggtttgtcg gaggctcgat tcaagcgggg
     2101 acactatagc tcatggctat ggatttggcg cgtttgccaa aaggacacac tccttaaaga
     2161 agcgaagccg ttaaatgcac gctgaggatg catccggccc cagcgggcgc agccgaatac
     2221 ctctgcgtgt cgattgcaac cgggaaaacg tcagcgacga aaagcgccgc tctgtgaacc
     2281 acctcaccgt caaaggccga ggtcgatggc aggctcccac gcgagcgttg atcccgcgag
     2341 gtggacccca tgcaaatgca ccagatccgc tacttcctag cgctctgcga ggagcgcaat
     2401 ttcacgcgag cggccgggcg ctgcggcgtc tcccagccct cgctgaccaa cgccatcata
     2461 gcattggagc gcgagctggg cggcgcgctt tttcaacgca agcccctgat cgccctcacc
     2521 gcgctcggcc gcgccataca gccttatctc gaccggatcg ctcaaaacgc agcgcatgcg
     2581 cgcgaggccg cgcaggcgct ggccgatgtg ccgccggcga attcggagcc tgcgccgcgc
     2641 gaggagatcg ccgggag
//
LOCUS       DATLJT010001064         1837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4439823,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001064 DATLJT010000000
VERSION     DATLJT010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4439823"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VM910_24405"
     CDS             <1..342
                     /locus_tag="VM910_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HVI65698.1"
                     /translation="TIVVYLVALPFVLVAGAGFLVFFIATAWLLGKQYFELAAMRFRS
                     PAETRMMRKDNALTIFTAGLIIAAFVSIPIVNLATPLFGMAFMVHMHKLLSGSRPEVI
                     EQSRRTSVPSA"
     gene            complement(403..1836)
                     /locus_tag="VM910_24410"
     CDS             complement(403..1836)
                     /locus_tag="VM910_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVI65699.1"
                     /translation="MPTIQDALGISPEAMSWIQTAYLIAEVIAIPLTGVLTRVLTLRW
                     LFAGAVCIFTLASIGCAFSQDFSVLLVFRVIQGFSGGVLIPAVFSAVFLLFPQHLHAV
                     ATTIGGVVAVLAPTIGPVVGGFITDTWSWPWLFLINVIPGLIAALLTPFLLPRDGTDF
                     AELKKLDVRALVLMAAALAFLEIALKQAPQNGWLSLPCAGLLLASMIGFAVFVWRSSY
                     AQHPVLRLVALERRSFAVGCALSFCLGVGLFGSVYLMPVFLAFVRGHDAFEIGTIMLV
                     TGVAQLVGAPLAVILESRLGARPVTALGFALFALGLGLSSLQPRIADFDEMFWPQIVR
                     GIAIMLCLLPPTRFALGELPEGEVADASGLFNLMRNLGGAIGIALIDTILYGRVAIHA
                     MDFRDRLLAGDVSAAEAIGLNPELFINRPPGPPDDAAIAYVRPMVEKASLALCVNEAW
                     ALLACVAVAGVLLVPFARDVTRSNARG"
BASE COUNT          410 a          579 c          580 g          268 t
ORIGIN      
        1 acgatcgtgg tctatctggt cgcgctgccc ttcgtgctgg tcgccggcgc gggttttctc
       61 gtgttcttca ttgcgacggc gtggctgctc ggcaagcaat atttcgagct cgcggccatg
      121 cgtttccgct cgcccgccga aacgcgaatg atgcgaaagg acaacgccct gacgatcttc
      181 acggcgggcc tcatcatcgc tgccttcgtg tcgatcccga tcgtcaattt ggcaacgccc
      241 ctgttcggca tggccttcat ggtgcacatg cacaagctgc tctcgggctc ccggcccgag
      301 gtgatcgagc agtcgcgccg gaccagcgtt cccagcgcat gatccggaaa agtgggcaca
      361 ggttttgcct cgcgacaaac gcgaaacgcg tttgcgtgga gatcatcccc tggcgttaga
      421 tctcgtaacg tcgcgcgcaa acggcaccag aagaacgccg gcaactgcca cgcaggcgag
      481 cagcgcccag gcctcgttga cgcagagtgc gagggacgcc ttttcgacca tcgggcggac
      541 ataggcgatg gctgcgtcat ccggcggtcc cggaggccga ttgatgaaga gctcgggatt
      601 gagccctatt gcctcggcag cgctcacgtc acctgcaagc agacgatcgc gaaaatccat
      661 ggcatggatt gcgacccggc catagaggat cgtgtcgatc agcgcgatgc cgatggcgcc
      721 accgagattg cgcatcaggt tgaacaatcc actggcgtca gcaacctcgc cttcgggaag
      781 ctcgccgagc gcgaagcgag tgggcggcag caggcagagc atgatggcaa tgccgcgcac
      841 gatttgcggc cagaacatct cgtcgaaatc ggcgatacgc ggctgcaagg aactgaggcc
      901 aagcccgagg gcgaacaacg caaagccaag ggccgtcacg gggcgagctc ccagacggct
      961 ctccaggatg accgcaagcg gcgcgccgac gagctgcgcg acgcccgtca ccagcatgat
     1021 cgtgccgatc tcgaaggcgt cgtggccgcg aacgaaggca agaaacaccg gcatgagata
     1081 gaccgagccg aaaagcccga cgccgaggca gaagctcaaa gcgcaaccga ccgcaaagga
     1141 gcgccgctcg agcgccacga gccgcaggac gggatgctgc gcataagatg accgccacac
     1201 gaacacagcg aagccgatca tgcttgccag cagcaggccc gcgcaaggaa gcgacagcca
     1261 gccgttctgc ggcgcctgct tcaacgcgat ctcaaggaac gcaagtgcgg ccgccatcag
     1321 gacgagtgca cgcacatcga gctttttcag ctcagcgaaa tctgtgccgt ctcgcggcag
     1381 gaggaagggc gtcagcagcg ctgcgatcag tccgggaatg acgttgatca ggaacaacca
     1441 cggccacgac caggtatccg tgatgaagcc gccaacgacc ggcccgatcg tgggggcaag
     1501 caccgcgacc acgccgccga tggtggtagc gacggcatgc agatgctgcg ggaacagcag
     1561 gaagaccgcc gagaacaccg cagggatcaa cacgccgccg gaaaagcctt gtatgacgcg
     1621 aaacaccagc agcactgaaa agtcctggct gaaggcgcaa ccgatcgagg ccagcgtgaa
     1681 aatgcagacc gcacccgcga atagccatcg cagggtcagg acccgcgtca atacgccggt
     1741 caaggggatc gcgatgacct ccgcgatcag ataggcggtc tggatccagc tcatcgcctc
     1801 cggcgaaatg ccgagcgcat cctggatcgt cggcagc
//
LOCUS       DATLJT010001065         5136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2602963,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001065 DATLJT010000000
VERSION     DATLJT010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2602963"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..389
                     /locus_tag="VM910_24415"
     CDS             48..389
                     /locus_tag="VM910_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HVI65700.1"
                     /translation="MQIPKQALLLRIFIGEDDKFKGSPLYEAIVTKAREMHLAGATVL
                     RGPMGFGASSRLHTTKILRLSEDLPLVIEIVDSEDKIDGFLPVLDGMMSSGLITLEKV
                     QVLQYGTKSTA"
     gene            1511..3166
                     /locus_tag="VM910_24420"
     CDS             1511..3166
                     /locus_tag="VM910_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HVI65701.1"
                     /translation="MATAETAGTMTGAGVGILDKERTIAAAGFNRWLVPPAALCIHLC
                     IGMAYGFSVFWLPLSRAIGLTAPKACPDISLVQELFTTTCDWKVASLGWMYTLFFVFL
                     GVSAAIWGGWLERVGPRKAGVVAACCWAGGLLIGAIGVYVHQLWIMWLGSGVIGGVGL
                     GLGYISPVSTLVKWFPDRRGMATGMAIMGFGGGAMIGAPLADLLMNYFKTPTSVGVWE
                     TFVTMGAIYFVFMMIGAFRYRLPPVGWRPEGWTPPSETKSMISQHHVHLDNAHKTPQF
                     WLIWWVLCLNVSAGIGVIGMASPMLQEIFAGKLIGLPHLTFGQLSVEQKTLIAGIAAG
                     FAGLLSLFNIGGRFFWASLSDYIGRKNTYYTFFLLGIALYALAPTFANMGSKALFVLG
                     FGIILSMYGGGFATVPAYLADMFGTQFVGAIHGRLLTAWSTAGIIGPVVVNYLREFQL
                     AAGVPRDKLYDSTMYVLCAMLVAGLICNYLIKPVNPKWYMSEAEVAKLQAASAKSQAG
                     VQHGSFGIGKGGLDGKAALFWLFVGIPLAWGVWKTLLSAAKIF"
     gene            3246..3530
                     /locus_tag="VM910_24425"
     CDS             3246..3530
                     /locus_tag="VM910_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65702.1"
                     /translation="MFRTGACLAAMLLAASLQTATAQTTTAPASSSPPPASTTAKPSK
                     TKLTMEKLKEMKAKWSSNRPKLKACRAQVKAKGLIGDDRWFFIADCMEKG"
     gene            3679..>5136
                     /locus_tag="VM910_24430"
     CDS             3679..>5136
                     /locus_tag="VM910_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVI65703.1"
                     /translation="MTTDVHQVRSFEHPGQGRKRAKATPKGRQVDPTAAEEIELLLGD
                     RPRRRDLLIEHLHLIQDKYHQISAAHLAALADAMKLSFAEVFETATFYAHFDVVKEGE
                     PDIAPLTIRVCDSLTCAMLGGEKLLQDLQSKAGPTMRVVRAPCVGFCDNAPVAEVGHR
                     YVLNATTSEVLATAARGETDPIIPSYVDYDAYVKDGGYKLLRDLRAGRIKKEDVLKAL
                     DDSSLRGLGGAGFPTGRKWRAVLGEPGPRVMVVNADEGEPGTFKDRFYLLRDVHRFLE
                     GTLIGAEIIDASDVYIYLRDEYPAAIKLLPLEIAKLPAGGPTVHIRRGAGAYICGEES
                     SLLESIEGKRGLPRHKPPYPFQVGLFGLPTLINNLETLFWVRDIVEKGADWWKSYGRN
                     GRFGLRSYSVSGRVKDPGVKLAPAGITVRELIDEYCGGMADGHTFRAYLPGGASGGIL
                     PASMDDIPLDFGTLEKYGCFIGSAAIVILSQQDSVK"
BASE COUNT          945 a         1530 c         1638 g         1023 t
ORIGIN      
        1 gggcatgtgc tggcaagccg ccttaaccgg ttgaagggga gctgacgatg caaattccga
       61 aacaggcgct gttgctgcgg atcttcattg gcgaggacga caagttcaag ggatcgccgt
      121 tgtatgaggc gatcgtcacg aaagcgcgcg agatgcacct tgccggtgca accgtgctgc
      181 gtggtccaat gggctttggc gcatcgagcc ggctgcatac cacgaaaatc cttcggctct
      241 cggaggacct tcccttggtg atcgagatcg tcgacagcga ggacaagatc gacggcttcc
      301 tgccggtgct cgacggcatg atgtcgagcg gcctgatcac gctcgagaag gtccaggtgc
      361 tgcaatatgg gaccaaatcc acggcctgac ggcacgaaac gcaaattgtg catgtccgtt
      421 atgcgaaatt tgcccgtcgt gccaatctgc cgcaggcgtg ccgcattggc gaaggaccca
      481 aatcgcaagc atgatccgcg cgtcccggcc tcgagtaaga ggggcgtatc gcgatcgtca
      541 cgaaacgtgg ggcgggatgc gatggacgcg gcacgtcaac tgacgagtga cgtcgtgcgc
      601 ggacgacgaa gtcgtgtggt accagtgccg ccgatttgaa gttcctgcag agttgccgca
      661 aggccgcaac tggaacttca aatcgaaagc ggcactggaa atattgattt tgctagcgcc
      721 ctttgctttc cgaagttcgt gaaagtggat gctgtgcgca cgaactttgg aaagcgggcg
      781 ctagctgcgc caaggctgga atcagctttg cggattggcg accgtcggcc ctatcgggcc
      841 cgacgcgccg agatgtgcag gcgacggaga caacaaagtc tggtctccgg ggagagtacg
      901 aagaaagtcg taaaccagcg tgcagggaat gccgatgttt cggttgtacc tgtggtgact
      961 taagcttgtg tgctcttccc aatcgcacac aaggctgcgg gtccagcaaa cgcccggcgt
     1021 tccctgcgcc ctccttagcg agggacgatg attttgatta aaaactcggg cgaatttcgt
     1081 cgcgggaaat acgacgcgcc tgtccaaggg cggcgcgtcg ctctgcgaaa ttcagtcgca
     1141 ggcgcggccg catcaggacg agaagctgac ataaagccgc catttcctat atccaaagac
     1201 acccccgaaa atcgaccttt tgcctatcga caggtgagac ggcttctgtc ccaattcgtc
     1261 ttctccgcaa ggacgcggag gttgagtcgt gccccggtta cgcgtaggcg cgctcagatc
     1321 ctttcggccc gcgtggcggc gctgggcata atgcgccgca acaggggttt gcattttcgg
     1381 atgcgctttc cggtcctggc gcagaacgag aatgatgcag agcagcaaag cggggcgcgc
     1441 ctgcgaggct ggcacaacag gatcacagaa ggcagagccg tccggcacgg gcggctgcat
     1501 ggaggaatag atggctactg ctgaaaccgc tggcaccatg accggcgccg gggtgggaat
     1561 cctcgataag gagcgaacga tcgcggccgc cggcttcaat cgttggctgg tgccgccggc
     1621 cgcgctctgt atccatcttt gcatcggcat ggcttacggc ttctcggttt tctggctgcc
     1681 gctgtcgcgc gcgatcgggc tgaccgcgcc gaaagcctgc ccggacatct cgctcgtcca
     1741 ggagcttttc accaccactt gcgactggaa ggtcgcgagc ctggggtgga tgtacacgct
     1801 gttctttgtg ttccttggcg tctccgctgc gatctggggt ggctggctcg agcgcgtcgg
     1861 accccgcaag gcaggtgtcg tcgccgcctg ctgctgggcc ggcggattgt tgatcggcgc
     1921 gatcggcgtt tacgtgcatc aactctggat catgtggctc ggctccggcg tgatcggcgg
     1981 cgtcgggctc gggctcggct acatctcgcc ggtgtcgacg ctggtgaaat ggttccccga
     2041 ccggcgcggc atggcaaccg gaatggccat catgggcttt ggcggcggtg cgatgatcgg
     2101 cgcgccgctc gccgatctct tgatgaacta cttcaagact ccgacctcgg ttggcgtctg
     2161 ggaaactttc gtcacgatgg gcgcgatcta tttcgtgttc atgatgatcg gcgccttccg
     2221 ctatcggctt ccgccggtcg gctggcgtcc tgagggctgg acaccgccga gcgaaaccaa
     2281 gtcgatgatt tcgcagcatc acgtgcacct cgacaacgcc cacaagacac cgcagttctg
     2341 gctgatctgg tgggtgctct gcttgaacgt gtccgccggc atcggcgtga tcggcatggc
     2401 ctcgccgatg ttgcaggaaa tcttcgcggg caagctgatc gggctgcccc acctgacctt
     2461 cggccaactc tcggtcgagc agaaaacatt gatcgcgggt atcgccgcag gcttcgccgg
     2521 tctcctgtcg ctgttcaata tcggcggccg tttcttctgg gcgtcgctgt ccgactatat
     2581 cggccgcaag aacacctact acacgttctt cctgctgggc attgcccttt atgcactggc
     2641 gccgaccttc gccaatatgg gttcgaaggc gctgttcgtg ctcggcttcg gcatcatcct
     2701 gtcgatgtat ggaggcggtt tcgccacggt gccggcctat ctcgccgaca tgttcggcac
     2761 gcagttcgtc ggcgccatcc acggccgcct gctgacggcc tggtcgaccg ccggcatcat
     2821 cggcccggtg gtggtgaatt atctcagaga gttccagctc gccgccggcg ttccccgcga
     2881 caagctctac gacagcacca tgtacgtgtt gtgcgcgatg ctggtcgcgg gcctgatttg
     2941 caactatctg atcaaacccg tgaatccgaa gtggtacatg agcgaggcgg aagtcgcgaa
     3001 gctgcaggcc gcaagtgcca agagccaggc tggtgtccag cacggctcgt tcggcatcgg
     3061 caagggcggt cttgacggca aggccgcgct gttctggctg tttgtaggca ttccgctcgc
     3121 ttggggcgtt tggaagacct tgctgagcgc ggccaagatc ttctgatccg gtttcgccgc
     3181 ggggcgtaag gcgtcccgcg gcggtctttc ttgatcaatc aaaaatgagc ttagggggat
     3241 tttccatgtt tcgtaccggc gcctgcctcg ctgccatgct gctcgccgcc agccttcaaa
     3301 ccgcgacggc ccagaccacg acggcgccgg cttccagctc gcctccgccg gcctcgacta
     3361 ctgccaagcc gtcgaagacg aagctgacga tggagaagct gaaggagatg aaggccaaat
     3421 ggtcctccaa caggcccaag ctgaaggcat gccgtgcgca agtgaaggcc aaggggctga
     3481 tcggcgacga ccgctggttc tttatcgcgg actgcatgga gaagggctga tcgcccggga
     3541 ggctacggag tttgctgtga ggggcagggc agtggaacgc tgcccgcccc ttaaatcatt
     3601 attcaagcac aattattctc ttggcatctg gcatgccagt aactagagtc ttaattgaga
     3661 gcaggaacga gacgtacgat gactactgac gtgcaccagg ttcgctcgtt cgaacatccg
     3721 ggccagggac ggaagcgggc gaaggcaact cccaagggcc gccaggtcga ccccactgcc
     3781 gcagaggaga tcgaactgtt gcttggcgac cggccgcgcc ggcgcgacct cctgatcgag
     3841 catctgcacc tgatccagga caaatatcac cagatctcgg cagcgcatct ggcagccctt
     3901 gcggacgcga tgaagctgtc cttcgccgaa gtgttcgaga ccgcgacctt ctacgcgcat
     3961 ttcgacgtgg tgaaggaggg cgagcccgac atcgcgccgc ttaccatccg cgtctgcgat
     4021 tccctgactt gcgcgatgct gggcggcgaa aagctgctgc aggacttgca gagcaaggcc
     4081 ggtcccacga tgcgcgtggt gcgcgcgccc tgcgtcggct tctgcgacaa tgcgccggtc
     4141 gccgaggtcg gtcaccgcta cgttctcaac gcaaccacct ccgaggttct cgcgaccgcc
     4201 gcgcgcggcg agaccgatcc gatcattccg agctatgtcg attacgacgc ctatgtgaag
     4261 gatggcggat acaagctgct ccgtgacctt cgcgcaggac ggatcaaaaa ggaggatgtt
     4321 ctcaaggcgc tcgatgattc cagtctgcgc ggacttggcg gcgccggctt tccgaccggg
     4381 cggaaatggc gcgcggtgtt gggcgagcct gggccgcgcg tgatggtcgt caatgccgac
     4441 gagggcgaac ccgggacctt caaggaccgc ttttatctgc ttcgtgacgt gcatcgcttt
     4501 ctcgagggta cgctgatcgg cgcggagatc atcgacgcgt ccgatgtcta catctatctg
     4561 cgcgacgaat atcccgccgc gataaagctg ttgccgctcg agattgccaa actcccggca
     4621 ggcggcccga ccgtgcacat aaggcgcggc gccggcgcct atatttgcgg cgaagaatcc
     4681 tcgctgcttg aaagcatcga aggcaagcgc gggctgcccc ggcacaagcc gccttatccg
     4741 ttccaggtcg ggctgttcgg cctgccgacg ctgatcaaca atctcgagac gttgttctgg
     4801 gtgcgcgaca tcgtcgagaa gggcgccgat tggtggaaga gctatggccg caacggccgc
     4861 ttcggactgc gcagctactc ggtctcgggg cgggtgaagg atcccggcgt caagcttgcg
     4921 ccggccggca tcaccgttcg cgaactgatc gacgaatact gcggcggcat ggccgacgga
     4981 cacaccttcc gcgcttatct tccgggcggc gcctccggcg gcatcctgcc cgcgtcgatg
     5041 gacgacatcc cgctcgattt cggcacgctg gaaaaatacg gctgcttcat cgggtcggcg
     5101 gcgatcgtga tcctgtcgca gcaggacagc gtgaag
//
LOCUS       DATLJT010001066         3094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4439936,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001066 DATLJT010000000
VERSION     DATLJT010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4439936"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..613)
                     /locus_tag="VM910_24435"
     CDS             complement(170..613)
                     /locus_tag="VM910_24435"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HVI65704.1"
                     /translation="MSYSAAAPESKAGWEHFPHDADVGVQGRGATAAQAFEQAAYALT
                     AIITHAEVKPQVSVQIRCEAPDLELLLVEWLNAIIYEMAVRKMLFGRFAVRIDDGHLE
                     GTLWGEPVEVERHAPACEPKGATYTALRVAKNPDGTWSAACIVDV"
     gene            complement(610..1179)
                     /locus_tag="VM910_24440"
     CDS             complement(610..1179)
                     /locus_tag="VM910_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HVI65705.1"
                     /translation="METPVQIDFQGMQGRPDVRDSIEKHVAELERRYGRVTACRVVVK
                     GPSGHHRTGGLYEVNIHLALPNGREVNIGRTPPADERHADLWFAINDAFKHARRRLQD
                     QVRRLQGQVKQHESQPIATVKTIDPSGDFGFLEAVDGHEVYFHRNSVLDGAFDRLAVG
                     THVTFAEEMGEKGPQASTVELLGKHRLRL"
     gene            complement(1217..2038)
                     /locus_tag="VM910_24445"
     CDS             complement(1217..2038)
                     /locus_tag="VM910_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI65706.1"
                     /translation="MSYASVMVYVEADAVPERRVRLAASLANQFNAMLIGVCALAIPP
                     PVMADGMVMDEWTDDDIELMRAKLADKGEWFRGIAGGDHRRLDWHAALDFPAETLARA
                     ARSADVVIIGQVKAAGNAYRALDPGEAILKMGRPTLVVPEGVNSLRAEHIVIGWKDTR
                     EARRAVRDALPFLREATRVTIVEACGPGEEKTALGRLDDVTRYLSYHRINAGPKVMLE
                     QKGSGAQQLIRIAQEERADLLVTGAYGHSRLGEWIFGGMTRELLAGSPICCLMSH"
     gene            2209..3094
                     /locus_tag="VM910_24450"
                     /pseudo
     CDS             2209..3094
                     /locus_tag="VM910_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046331.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
BASE COUNT          570 a          973 c          909 g          642 t
ORIGIN      
        1 aagcgtcgcg acattcacgg cctgctcaaa gaccttgtcg tccatgtcgc ggatgaggtt
       61 ctcgtcggca taaataatgg ccggcacccg catggcgccg tgcggttcga tccgccatgt
      121 cgtggcgtcg acccgagtga agcgagcggg ctccatggcc gtttctccgc tagacgtcca
      181 cgatgcaggc ggcagaccaa gtgccgtcgg gattcttggc gaccctcaga gcagtatagg
      241 ttgccccttt cggctcgcag gccggggcat ggcgctcgac ctcgacgggc tccccccaca
      301 gcgtcccttc gaggtgcccg tcatcgatgc gcaccgcaaa gcgcccaaac agcattttcc
      361 ggaccgccat ctcatagatg atggcgttga gccattcgac caaaagaagt tcgaggtcgg
      421 gcgcctcaca ccggatctgg accgagacct gcggcttgac ttccgcatgg gtgatgatgg
      481 cggtcaaagc gtaagccgcc tgctcgaagg cctgcgccgc cgtcgcgccc cgtccctgca
      541 cgccgacgtc ggcatcgtgc gggaaatgct cccacccggc cttgctctcc ggcgccgcgg
      601 cagaatagct catagtctta gcctgtgctt gccgagcagc tcgaccgtgc tcgcctgggg
      661 tcctttttca cccatctcct cagcgaatgt gacgtgcgtg cccacggcaa gacggtcgaa
      721 tgcgccatcg agcacgctat ttcgatgaaa atagacttcg tggccgtcaa cggcctccag
      781 gaatccgaag tcaccggacg ggtcgatggt cttcacggtc gcgatcggtt gactttcgtg
      841 ttgtttcacc tgcccctgca aacggcggac ctgatcctgc agccgtcgtc gtgcatgctt
      901 gaaggcatca ttgatggcaa accaaagatc ggcgtggcgt tcgtccgccg gcggcgtgcg
      961 cccgatgttg acttcgcgac cattcgggag cgcaagatga atgttgacct catagagacc
     1021 gccggtccgg tgatgtccgc tgggcccctt caccacgacc cggcaagccg tgacgcggcc
     1081 gtagcgccgc tccagctcgg ccacgtgctt ttcgatcgaa tcgcggacgt ccggtcttcc
     1141 ctgcatgcct tggaaatcga tttgaacggg cgtttccata ttgtcgttat cctccgagag
     1201 ccgttggcta tgaaaatcag tgcgacatga ggcagcagat gggacttccc gcgagcagct
     1261 cccgcgtcat gccgccgaag atccactcgc ccaggcggct gtgcccgtag gcgccggtga
     1321 ccagcagatc ggcccgttcc tcctgagcga tccggatcaa ctgctgtgcg ccagatccct
     1381 tttgctccaa catgactttg ggtccggcgt tgatgcgatg gtacgataga taacgcgtta
     1441 cgtcgtcgag ccgtcccaat gcggtcttct cttcgcccgg cccacatgct tcgacaatgg
     1501 tgacgcgcgt tgcctctcgc aggaacggaa gtgcgtcccg caccgctcgg cgcgcttcgc
     1561 gcgtatcctt ccagccgatc acgatatgct ctgctcgcag cgagttcact ccctcgggca
     1621 caactagggt cgggcgaccc atcttcagaa ttgcttcgcc cggatcgagt gccctgtaag
     1681 catttcccgc tgccttcacc tgtccgatga tcacaacatc agcgctgcgc gccgcacgtg
     1741 ccagtgtctc ggcgggaaag tccagcgctg catgccagtc gagccttcgg tgatcacccc
     1801 ctgcgatgcc gcggaaccat tcgcctttgt cagcaagctt cgcccgcatc agttcaatat
     1861 cgtcatccgt ccactcgtcc atgaccatgc catcagccat gactggtggc ggaatagcga
     1921 gggcgcacac tccgatcagc attgcattga actgattggc aagacttgcc gcgagccgta
     1981 cgcgtcgctc cggcaccgcg tcagcctcga cgtaaaccat cacagatgcg taggacacgc
     2041 gccctcctgc ggttagctcg cgcgggctta ggtgaaaagc ggttcctaag ctgccagcgc
     2101 aagatagcaa accgggtcct gctcgcattt gacgtatgtc aacgtcgggc gcgcaaatcc
     2161 gcctctaatc agccccggct tacatgctgc aagggccgct caacgataat gacagccgtc
     2221 actaatattc ggtggttccg cgacatcggg ctcgacgatg tcgctgtggt gggcggaaag
     2281 aatgcgtcgc ttggcgaact ttattctgtc ctaacggggc agggtgtccg tgtcccgaat
     2341 gggtttgcgc tgacggccga cgcataccgc aacgcactga ccgcggctgg agcgtgggat
     2401 agactgcatc ggctcctcga cgcagtcgat aaggcgcgca tagacgtgct caggcggtgc
     2461 gctgccgaag cgcgcgcgat cgtctttgcc gctaccggta cggacaagct ccaacaagag
     2521 attgcggaat catatcgaag gctcgaacgt gactatggcg ccaacgttgc ggtcgccgtt
     2581 cgtagctccg ccacggcgga agaccttcca accgcaagtt ttgcaggtca acatgagagt
     2641 ttcttgaacg tccgcggcgc caccgatttg tttgaagctt gccgacgctg ctttgcttct
     2701 atcttcaccg accgggcgat ttcgtatcgg atcgataacg gcttcgatca tttcaaagtc
     2761 gcgctttcgg tcggggtcat gaagatggtc agagccgacc gtgctgcaag cggggtcgtc
     2821 ttcacgttgg acaccgaatc cggctttcgg gatgtcgtgt tcatcacggg gcgtctacgg
     2881 cttaggtgaa aatattgtcc agggaacggt cgacccggac gagttctacg tgcacaagcc
     2941 gacgttccgc cagggctatc gcgcggtctt gagccgatca ctcggacgca agcaaatgcg
     3001 catggtctat gcccgtgacc atggcgggac aagaaatgtt tcggttccga gccgggcccg
     3061 ggaacgcttt tgcatcgacg atgcggaggt gctc
//
LOCUS       DATLJT010001067         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_991937,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001067 DATLJT010000000
VERSION     DATLJT010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991937"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1108)
                     /locus_tag="VM910_24455"
     CDS             complement(266..1108)
                     /locus_tag="VM910_24455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI65707.1"
                     /translation="MTRIALVVSDVDGTLLTKNKTLTEGAKAAVQRLHEAGIGFTITS
                     SRPTIGMRFLIEPLKIKLPVGPFNGSAIVDPQMNPIEQHLIPTSAVRRSLELLGDFGV
                     DIWLFTHDQWLTRNGSGEYVPLEVRAIRADPTVVSDFSPYLSRACKIVGSSSDADLLM
                     RCEAAARSALGVDATAVRSQSYYLDITPPGCDKGTFVRAMAQRLGVSSDAVATIGDMQ
                     NDLPMFAASGLSFAMGNATDDVKRHAKRVTASNEEDGFAKAMDAILRDNGASNVPNSD
                     GFST"
     gene            complement(1105..1617)
                     /locus_tag="VM910_24460"
     CDS             complement(1105..1617)
                     /locus_tag="VM910_24460"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HVI65708.1"
                     /translation="MGVSGSGKSTVGEALAKRLGWLYEDGDKFHPASNVAKMSAGQPL
                     TDEDRWPWLRAIAGEIDRLSAAGVHAVIACSALRRVYRDILVHGRNDVRIVYLDGTQA
                     LIAERLRRRKGHFMPPELLKSQFKTLEPPDRDEHSVTASIDAPVEAIVDDILRQLDLR
                     PAGEKRITSP"
     gene            complement(1649..2395)
                     /gene="pgl"
                     /locus_tag="VM910_24465"
     CDS             complement(1649..2395)
                     /gene="pgl"
                     /locus_tag="VM910_24465"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVI65709.1"
                     /translation="MAPTDDRTLVTVADPVALAKAAADRLLARMAAQDGRIAICLSGG
                     SSPKRLYELLATETYREKIPWDRVHWFMGDERFVPPDNPLNNMAMARRTFLDSLAPSS
                     HIHPIPTDAADPDEAAARYERELKTFYGAEQLDPARPLFDLVLLGVGPDGHTASLFPD
                     YPALEEADRWVVGVEKAHVEPFVPRVTLTLPALASCREMLFEISGEQKRAILTRVLAG
                     EILPANRARANGETVWLVDRAALPGDSRGR"
     gene            complement(2398..3912)
                     /gene="zwf"
                     /locus_tag="VM910_24470"
     CDS             complement(2398..3912)
                     /gene="zwf"
                     /locus_tag="VM910_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVI65710.1"
                     /translation="MAGPQHRTKPDSCSFVIFGVTGDLTHRLVIPALYNLAASDLLPD
                     RICIVGIARRSISNEALRESLMKGLRQFATRPVEDKIANRLLECVTCVEADAGDPPSF
                     NRLKEQLERLEKARNTGGNRLFYMATPPDAFAPIARQLGRTGLVKEEHGAWRRLVVEK
                     PFGTDLESAKTLDAELLKILDEHQIYRIDHYLGKETVQNILVLRFGNGMFEPIWNRHH
                     IEHVQITVDEKIPVVAHRGSFYDATGALRDMVPNHLFQLLSLIAMEPPAHFDAHTVRT
                     EKGEVLEAIQTQSEEEALKNSVRGQYTAGKIDGKEIIDYRKIPSVRPDSTVETYVALK
                     LIIDNWRWAGVPFYLRTGKALATKRTEVAIKFREPPFAMFKCTPVEQLKENYFVIGVE
                     PTEGIDLQFNTKVPGPTVQISGVEMKFRYKDYFKAEPATGYETLIYDCMIGDNILFQR
                     ADSVEAGWQAVQPFLDAWKKAGANGLEFYPAGSEGPKGAFDLLKRDGRSWRKLT"
     gene            complement(3918..>4064)
                     /locus_tag="VM910_24475"
     CDS             complement(3918..>4064)
                     /locus_tag="VM910_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HVI65711.1"
                     /translation="AVPAEVLTAALFARFRSRKDHTFAEKILSAMRAGFGGHREPKKP
                     TSKG"
BASE COUNT          719 a         1236 c         1321 g          788 t
ORIGIN      
        1 accatgagga cgatcagtgc gacggcgatg ccgacgatct ttcgcctgat cgatggacgg
       61 aatatccagt tcatgggccc cgtggtgatg gttgcggaag gcttgcggcc gccagcatgc
      121 atcaatcccg gccattccgc caactgtgat ccgcgccgat gctgcgcaat gaccggggat
      181 caacaaggta aatgatggtc gggctccgga cgggcggatt ccggtacggt tttggccggc
      241 gagggctgcc caaaacgcaa tggcttcagg tcgaaaagcc atcgctattt ggcacgttgc
      301 ttgcgccgtt gtcgcgcaga atcgcgtcca ttgccttggc aaacccgtct tcctcgttcg
      361 acgccgtgac ccgcttcgcg tgcctcttga cgtcgtccgt cgcgttcccc atcgcaaacg
      421 acaggccgct tgccgcaaac atcggaaggt cgttctgcat gtcgccgatg gtggcgacgg
      481 catcgctgga cacgccgagc cgctgagcca tggctcgaac gaaggtgccc ttgtcgcaac
      541 caggcggcgt gatgtcgaga taatagcttt gcgagcggac agcggtggca tcgacgccga
      601 gcgcgctccg cgctgccgct tcgcaacgca tcagcaaatc ggcatcggag gacgagccga
      661 cgatcttgca ggctcgcgat agataaggcg aaaaatcgga aacgacggtc ggatcggcgc
      721 ggatcgcccg tacttcgagc ggcacatatt cgccgctacc gttgcgcgtg agccattggt
      781 cgtgggtgaa cagccagatg tcgaccccga aatccccgag caattccaga ctgcgccgga
      841 cggcggatgt cgggatcaga tgctgctcga tcgggttcat ctgcgggtcg acgatggcgc
      901 tcccattgaa agggccgacc ggcaacttga tcttgagcgg ctcgatcaaa aaacgcatgc
      961 cgatggttgg acggctggag gtgatggtga agccgatgcc ggcctcgtgc agcctttgca
     1021 ccgcggcttt ggcgccctcg gtgagagtct tgttcttcgt caacagcgtg ccgtcgacat
     1081 cggaaaccac cagtgcgatc cgggtcatgg gctggtgatc cgtttctcgc cggcggggcg
     1141 caaatcgagc tggcgcagga tgtcatccac gatggcttcg accggagcgt cgatcgaggc
     1201 cgtcactgaa tgctcgtcgc ggtccggcgg ctccagcgtc ttgaattggc tcttcagaag
     1261 ttcgggcggc atgaaatgac ccttgcgccg gcgcaagcgc tccgcgatca aggcttgcgt
     1321 accgtcgagg taaacgattc ggacgtcgtt tcgtccgtgt acgagaatgt cgcggtagac
     1381 ccggcgcagc gccgagcagg cgatcaccgc gtgcacgccc gcggcactca ggcgatcgat
     1441 ctcgccggca atcgcgcgca accagggcca gcggtcctcg tcggtcaggg gttggccggc
     1501 gctcatcttg gcgacgttgc tggcggggtg gaatttgtcg ccgtcctcgt agagccagcc
     1561 caggcgtttg gccagtgcct cgccgacggt gctcttgccc gatccggata cgcccatcac
     1621 gatgagggcg cacagcgggc ttgcgacatc agcggccacg agaatctccc gggagcgcag
     1681 cgcgatccac cagccaaacg gtctcgccat tggcgcgcgc gcgattggcg ggcaggatct
     1741 cgcccgcgag gacgcgcgtc aagatcgcgc gcttctgttc gcctgatatc tcgaacagca
     1801 tctcgcggca ggaggcgaga gcaggaagcg tcagcgtgac ccgcggcacg aagggctcga
     1861 cgtgcgcctt ctccacgccg accacccagc gatcggcctc ctcgagggcc ggataatccg
     1921 gaaacagcga agccgtgtga ccgtcgggac ccacgcccaa cagcaccagg tcgaacaagg
     1981 gacgcgccgg atcgagttgc tcggcgccgt agaaggtttt cagctcacgc tcgtagcgcg
     2041 cggcggcttc gtcaggatcg gcggcgtcgg tcgggatcgg gtggatgtgg gacgaaggtg
     2101 caagggaatc gagaaaagtc cgtcgcgcca tcgccatgtt gttgagtgga ttgtcgggcg
     2161 ggacaaagcg ttcgtcgccc atgaaccagt gcacgcggtc ccacggaatt ttttcgcgat
     2221 aggtctcggt tgcgagcaac tcatacagcc tcttcgggct cgagccgccg gagaggcaga
     2281 ttgcgatgcg gccgtcttgc gctgccatcc gcgccagcag acgatcggcg gcggccttcg
     2341 cgagcgcgac gggatcggcg acagttacca gcgtgcgatc atcagtgggg gccatcgtca
     2401 ggtcagcttg cgccagcttc gcccatcccg cttcaataaa tcaaacgcgc ccttaggtcc
     2461 ctcgctgccc gcgggataga actccagacc gttcgcgccc gccttcttcc aggcatcgag
     2521 gaaaggctgt acggcctgcc agccggcctc gacgctgtcg gcgcgctgga acaggatgtt
     2581 gtcgccgatc atgcaatcat agatcagcgt ctcgtagccg gtcgcaggct cggccttgaa
     2641 atagtccttg tagcggaact tcatctcgac gccgctgatt tgcacggtcg gccccggtac
     2701 cttggtgttg aactgcaggt cgatgccttc ggtcggctcg acgccaatga cgaaataatt
     2761 ctccttgagc tgttcgaccg gcgtgcattt gaacatcgcg aacggcggct cgcgaaactt
     2821 gatcgcgacc tccgtgcgct tggtggcgag cgcctttccg gtgcgcaaat agaagggcac
     2881 gccggcccag cgccagttgt cgatgatcag cttcagcgcg acgtaggtct cgaccgtgct
     2941 gtcgggcctg acggagggga tcttgcgata gtcaatgatt tccttgccgt cgatcttgcc
     3001 ggcagtgtac tggccgcgca ccgaattttt cagtgcttcc tcttcgctct gggtctggat
     3061 ggcttccagc acctcgccct tttcggtgcg aacggtgtgc gcgtcgaaat gcgccggcgg
     3121 ctccatcgcg atcagcgaga gcaactggaa gagatggttc ggcaccatgt cgcggagggc
     3181 gccggtggcg tcgtagaaac tgccgcggtg cgcgacgacg ggaatcttct cgtcgacggt
     3241 gatctgcacg tgctcgatgt ggtggcggtt ccagatcggc tcgaacatgc cattgccgaa
     3301 gcgcagcacc aggatgttct ggacggtttc cttgccgagg taatgatcga tccgatagat
     3361 ctggtgctcg tcgagaatct tgaggagttc cgcatccagg gtcttggcgg actccagatc
     3421 ggttccgaac ggcttttcca cgaccagccg ccgccacgcg ccgtgctctt cttttaccag
     3481 gccggtacga ccgagctggc gcgcgatcgg agcgaaagcg tcaggcgggg ttgccatgta
     3541 aaacaggcga ttgccaccgg tgttgcgcgc cttttccagt cgctcaagct gttccttcag
     3601 ccggttgaat gagggcggat cgccggcgtc ggcctcgacg caggtgacgc attcgagcag
     3661 gcggttggcg atcttgtctt ccacggggcg cgttgcgaac tgccgcagcc ctttcatcag
     3721 actctcgcgc agcgcctcgt tcgagatgct cctgcgggcg atgccgacga tgcaaatcct
     3781 gtccggcaac aggtcgctcg cggcgaggtt gtaaagcgcg gggatcacca gccgatgcgt
     3841 cagatcgccg gtcacgccga agatgacgaa tgagcaggaa tccggtttcg tccgatgttg
     3901 aggaccggcc atgcgtctca accctttgac gttggctttt tgggctcccg atgcccgccg
     3961 aagccggcgc gcattgccga gagaatcttt tcggcgaagg tgtggtcttt ccgcgagcgg
     4021 aagcgcgcga acaaggccgc ggtcaggact tccgcgggta ccgc
//
LOCUS       DATLJT010001068        11381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3520928,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001068 DATLJT010000000
VERSION     DATLJT010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3520928"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(729..1577)
                     /locus_tag="VM910_24480"
     CDS             complement(729..1577)
                     /locus_tag="VM910_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65712.1"
                     /translation="MAQRSLDLIEAMYAAAEAAQPITGRGVGYKLFSAGMIPSMGRAD
                     MRRVYRLLKEARERGHVPWAWIVDETRELERVSTWANPESYARCVARSYRRDFWNQQP
                     YRILVVSEKGTVRGLLRPVLDHFAVGFQVMHGFSSATIVHDISEDDDGRPLIILYVGD
                     FDPSGMYMSEEDLPNRFSKYDGAHVTLRRIALTREHVRGLPSFPATDKRKDPRYKWFV
                     RNYGKRCWELDALDPNDLRDCVEQEIKKLIEPVAWKRCEIVNRAEQDSLKTILEKWGA
                     PRARDD"
     gene            complement(1622..1792)
                     /locus_tag="VM910_24485"
     CDS             complement(1622..1792)
                     /locus_tag="VM910_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65713.1"
                     /translation="MTPAPKLTPTEKAAKALAQLSDAEWTKVKRDEERRRAQMGVIRN
                     LSREHRDREPVR"
     gene            complement(1789..2130)
                     /locus_tag="VM910_24490"
     CDS             complement(1789..2130)
                     /locus_tag="VM910_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65714.1"
                     /translation="MSERQFAISGRWALAADSLQWMLQRRAGQRWQSVSFVSSTRDVL
                     LRCMREKGCPPADAERLLAGLPSTFEEWAGKRVQNPATADLADGEGHHADTPLPNAAN
                     ARRDRDPEPAR"
     gene            complement(2249..2488)
                     /locus_tag="VM910_24495"
     CDS             complement(2249..2488)
                     /locus_tag="VM910_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65715.1"
                     /translation="MPRQVTSEVAEALDNERKALAAPPAPEPIAKCCQKNARRLSGVK
                     VEISHNWHCRPLLRARRERPRGRRAAEQRDELAAL"
     gene            complement(3291..3452)
                     /locus_tag="VM910_24500"
     CDS             complement(3291..3452)
                     /locus_tag="VM910_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65716.1"
                     /translation="MDRLVLREQKAFIDGLARQELLEEDQLAELRKLLARQAVYDVDR
                     WLAAFGHKR"
     gene            complement(3440..3628)
                     /locus_tag="VM910_24505"
     CDS             complement(3440..3628)
                     /locus_tag="VM910_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65717.1"
                     /translation="MVSALKSSTKLKFAGRKVNVELPALRPTVKLADPSTWAPPPRKL
                     PERWKMIERLSDHREWTA"
     gene            3719..4756
                     /locus_tag="VM910_24510"
     CDS             3719..4756
                     /locus_tag="VM910_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65718.1"
                     /translation="MRILAAAVIIPAIAGAVAWGFIESRQGREVETKRERAVKAPLRV
                     SNANGAAVIKVDAEEQERNGIQTTALLPAPYQEEVRAYGMVLDLARLTDLSNNYANAK
                     SQLETAQAKVAASKAAFERAQNFYKDGKTISLAQFQAAEAAFRVDDAAVGAAQSRVRT
                     LRATAEQEWGPALGKSLIDGSRIVTRLIERQDFLLQVTLPPSVSIPTPPVTASIETGK
                     GERAAITFISPATRTDPKIQGISFFYIASAESGVLPGMNVLALLPTGTTVGGVTVSAP
                     AIVWWQDRAWAYRRAGPNTFTRTEISTSLPAPGGGYIVKDLPTNSEIVTQGAQLLLSE
                     EFRAQIQVGGD"
     gene            4759..7917
                     /locus_tag="VM910_24515"
     CDS             4759..7917
                     /locus_tag="VM910_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI65719.1"
                     /translation="MSPTLPSGPQAGLITFAIRFRGIVIALACMLLAYGGYSLGRVNY
                     DVFPEFAPPQVGIQTEAPGLTPEQVEILVTRPIENAINGVSGVQKLRSTSIQGLSVVT
                     VFFDPSSDIYRDRQVVAERLAVAAHQLPLGVQPPAMTPLTSSTSTVLVIGLTSQAQSL
                     MKLRTIADWTVRPRLLAVPGVAKVVTFGGHSRSIQVQVHPDEVIRYNLSLNDVLAAAR
                     RATGVRGAGFLDTKNQRVVFQTEGQSLAVDDIARTVLLSQGAASVTLGNVAEVVEAPE
                     PPIGGAAIDGQLGVIINVSAQYGANTVAVTNAVEAALNELRPGLEAEGIVLHADLFRP
                     ANFINTATSNVQQSLILGAGVVIVVLFLFLFDLRSAAISCSAIPLSLLTAIIVLERLG
                     ATLNTMTLGGLAISIGVVVDDAVIDVENIARRLRENGRLPQPRPIPRVVLDACLEVRS
                     AVVYATFAVILVVLPVMALSGIAGRLFAPLGLSYSLAVMASLVVALTVTPALSMALLK
                     DRHAPTTDPPVVRWARSGYERLLRCIARRPRTVIAAAFVFTVAGCSGLPFFGGSFIPE
                     LKEDHYTLHMSAVPGTSIEESLRIGIRVADALKRLPSVRSVAQRVGRAERAEDTWGTH
                     YSEFEIDLKPLSGDETETAEAEIRRTLAAFVGVNFSLKTFLTERVEETLSGYTASVAV
                     NVFGNDLDVLDQKARDIAIELGEVPGATEVQIQAPPGLPQLTIRLRKTDLERWGFDAV
                     EVLELIRAAYQGEIVGQTYQGNQVFNVITVLDRASRDSITKVGGLPLRSQGGTFVLLR
                     QVADIYQAAGRYQVLHEGAQRVQTVTANVAGDDVVSFVQAAKPKIATEVELPAGTYLQ
                     FAGAAEAQSQSRRDLIINSLIASAGIILLLSIATCNWRNLTLILANLPFALVGGVLAV
                     FVTGGQLSLGGMVGFVTLFGITLRNSVLMIAHYEHLVAVENMSWGLDTAIRGAADRLS
                     PILMTSIVTALGVLPLAIGMGDPGREIEGRMAVVILGGLLSSMALNLLVLPTLSLRYG
                     KFEPSRDEFEQAGAPSVRSVAE"
     gene            complement(8654..9505)
                     /locus_tag="VM910_24520"
     CDS             complement(8654..9505)
                     /locus_tag="VM910_24520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVI65720.1"
                     /translation="MSARLDHGEDTLDEMHEINVTPFMAEDRADLRRWVMCGAVIVFA
                     HGAIAAGMVTWRDEIEPAEPAAAIVIEFAPMPVAPAAPQTEIPPGPEQVMSDASPNKP
                     VKSLEEKEKVEEKVESKPVEEPPPEIKPAPNPDVAVEPPPQEVPTPQRQEPRPPAPTT
                     SAPQALPDQTAAIPAAPIQGRITPHNSNAVPTWKTQIVALLERNKRYPAVAQSRREQR
                     IAQVFFSLDRKGRVIDSRISRSSGASALDEEALALLRRAQPFPAPPRELPGDRVDLTV
                     PIRFNLR"
     gene            complement(9516..10319)
                     /gene="exbB"
                     /locus_tag="VM910_24525"
     CDS             complement(9516..10319)
                     /gene="exbB"
                     /locus_tag="VM910_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HVI65721.1"
                     /translation="MLRGAAMIALLIVPLPALAQDNVVAIATLPRDLSPWGMYLNADL
                     VVKAVLIGLAFASIVTWTVWLAKTIEIFFAKRRVREALNKLVHVRSTADGVERLADSR
                     GEVMQFLDAAVTELKVSSGSIDREGIKERIASRLERIEASYGRRILRGTGVLATIGAT
                     APFVGLFGTVWGIMNSFIGISKSHTTNLAVVAPGIAEALLATAFGLAAAIPAVVIYNV
                     FARSIAGYRAGVGDAAAQVLGLVSRDLERVVVPAPSARRAPPLSAVERT"
     gene            complement(10368..11000)
                     /locus_tag="VM910_24530"
     CDS             complement(10368..11000)
                     /locus_tag="VM910_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI65722.1"
                     /translation="MTSVLIIDDHPIVLQGLRRVLEDAGVNPVLEARDVTSGYRLFRR
                     NRPDVIVVDLGMQGSVLGGLDLIRRMRSHEPCTCILVFSMHNDPMIAARALEAGATGY
                     LLKDRSSDELMKAFEQVRSGKPYLSEDLAMRVALLRTGVENPLVDLTPRQSQILSLLA
                     KGKTYSSIAEELGVSYKTVVNTCLHLKRTLGAKNLPELIRVAVRLLPADS"
BASE COUNT         2052 a         3612 c         3480 g         2237 t
ORIGIN      
        1 gcctcgaccg tagttgggca tgacgcgacg cgggagagag ggaattccga ccggcgtgag
       61 cctaaccccg gcggcggcga tttggcgaga gggcggctcg accgggccgc gccttcgctg
      121 tccccgccgc gatctcgccc accgccggga cacaagcggg acacggattt ttcaagccat
      181 tgctgcggct gtcgtttttc cgctgcgctc aagccctatc gcggcaacca gcggcctccg
      241 ctgttgcgat cggtttttcg ggggtcctcg agatatctag ccgggtcgaa taacgcatcg
      301 cgatcgagca caacctgcat ttcgaccgct aatcgatgac ttcccttcgg tgcggcggcc
      361 cccagtcggt ttcgttctct ttggtaagtc cgacaagcaa ttgctccaag gtatagcggc
      421 ggcgcttttt cttttgttcc ctccatgatc ggagccgcgt cgaccctgcc ggcgttctcg
      481 tagtcgactc tcgatatgat tgccattcaa gcatcctccg ccgaccgcat cctcggaagg
      541 gtgcgagccc ggcaccggat ttttggagtt cagtgcccct ttggccgggc tcgctacggc
      601 ggcggcaccc gcttcgcgtc atcgcagcct ctttcgtgcg atttccccga ccagccgcgt
      661 gatgatgtcg cgctcggact ctcgctagga aagcatgcgc gtccgcgcca aggccgagca
      721 cgagcgcgtc agtcatcgcg cgccctgggt gcgccccact tttcaaggat cgtcttcaac
      781 gaatcctgct ctgcccggtt gacaatctcg cagcgtttcc atgccacggg ctcgatcagt
      841 ttcttgatct cctgctcgac gcagtcgcgc agatcgttcg ggtcgagcgc atcaagctcc
      901 caacagcgtt tgccgtagtt gcgcacgaac catttgtagc gcgggtcttt acgcttatct
      961 gttgccggga acgatgggag cccgcgtaca tgttctcgtg tcagcgcaat tcgccttagc
     1021 gttacgtgag cgccatcgta tttggagaac cggttcggca agtcctcctc cgacatgtac
     1081 atgccgctcg ggtcaaagtc gccgacgtac aggatgatca atggacggcc gtcgtcgtct
     1141 tcggaaatgt cgtgaacgat ggtggcgctg ctgaaaccat gcatgacctg aaagccgacc
     1201 gcgaaatggt cgagcaccgg tcgaagcaaa ccgcgcacag tgcctttctc gctcaccacc
     1261 aggatgcggt acggttgctg gttccagaag tctcggcggt aggaccgggc gacacagcgc
     1321 gcgtatgatt cgggattggc ccaggttgag acccgctcca gctcgcgggt ctcatccaca
     1381 atccacgccc atgggacgtg cccccgctcg cgcgcctctt tgagcaggcg gtacacacgc
     1441 cgcatgtccg cgcgccccat cgaagggatc attccggcgc tgaacagctt gtacccgacg
     1501 ccgcgacctg tgatcggttg tgcagcctcg gccgcggcgt acatcgcctc gatcaaatcg
     1561 agggatcgct gcgccatgcc ccgcccgcga ccctttttgg tactgggttc aaaatgctca
     1621 gtcatcgcac cggctcccga tcgcgatgtt cacgagacaa atttcgaatg acgcccatct
     1681 gggctcgccg tcgctcctcg tcgcgcttga ccttcgtcca ctcggcatcg gagagctggg
     1741 cgagcgcctt ggcggctttc tcggttgggg tgagctttgg agccggggtc atcgcgccgg
     1801 ctccgggtcg cgatcccgtc gcgcgtttgc agcgttgggg aggggcgtat cggcatggtg
     1861 gccttcccca tcggccaaat ccgctgttgc agggttctga acgcgtttcc cggcccattc
     1921 ctcaaaggtc gagggtaacc cggccagcag ccgctcggcg tcggcggggg gacagccctt
     1981 ctcccgcatg cagcggagga gcacgtcccg ggtcgagctt acgaacgaaa ccgactgcca
     2041 ccgctggcca gctcgacgct gcagcatcca ctgcaggctg tcggcggcca acgcccaccg
     2101 gccggagatc gcgaactgtc gctcgctcat gccgcgcccc cacagcaaga gggcgccgtc
     2161 ccagccactg ggcaaaatac gctaagactg gcaaagtgcg ctttaagcgc acccagccag
     2221 cgcaagccct tggaatcaca tgatctggtt acaacgccgc gagttcatca cgctgctcgg
     2281 cggcgcggcg gccgcgtggc cgctcgcggc gcgcgcgcag cagcgggcgg caatgccagt
     2341 tgtgggatat ctcgactttt acgccgctga gccgacgggc atttttctgg cagcatttcg
     2401 caatcggctc cggtgccggt ggcgcggcaa gcgccttgcg ttcgttgtcc agcgcctcgg
     2461 cgacctcgga cgtgacctgg cgcggcaccg ccagcgctta atcgctgtcg cgtatcgttg
     2521 gagacgaagt ccttgtgctg cacccttccc atgcccgaca tgtatgcggg ccaacagcgg
     2581 acggcgtgcg ccacttccgg ctcctcgcgc aaaagacggt cgcggtcggc agctagcagc
     2641 gtgcggaacg tctcgagcaa cgcccgttgc ctttcagccg tcgtgccctc cggcgcttgg
     2701 cttgagcgcc gccttcttcg ccgcgatctg cgtctcgaag tggtctgcta ggtcgacctt
     2761 gctacgccac gcgtcggacc attgctctag cgtcgagcgt cgatacacca gctcggccgg
     2821 tgtcggcggc cgcgccttgc ctcgcagctg cgcgtcatga agcgcgtcgt tgcgcgcgct
     2881 gaccgcctgc gcgatcgaaa cgaggtcgcg acgagcgccg cggaccttgc caacaattgt
     2941 agtcgccggg tatgccgctt cgttctcgaa tttatcggga agccacgcaa ggtagcccta
     3001 cttcccgtct gcggctgctg tgaaatccag cttgtctcac cgccatgtct cgcctcgtac
     3061 ttgctgttga taacgacgca aacccgcagc gcaggctttc tcgacgtcca cgtcgcggaa
     3121 ccccgccgcc tttagcttca aattggcgaa tacatgcgcg acgaatctcg cgcgcttctc
     3181 ttgatcaagc gaagcccgtg cggcaactgc cacgtcgcgt ggttcggcgg cacaagcttc
     3241 cgcggcgggt aaaggtgctt ggtattgctt ttcgccattg aagttgcctc ctatcgtttg
     3301 tggccgaaag cggccagcca ccgatcgacg tcatagacag cttgtcgagc cagcagcttg
     3361 cgcagttccg caagctgatc ctcttcgagt agctcttggc gcgcgagccc gtcgataaac
     3421 gccttctgct cacgaagaac taagcggtcc attcccggtg atctgacagc cgctcgatca
     3481 ttttccaccg ctccggcagc ttccgcggcg gcggggccca ggtgctcgga tcggcaagct
     3541 tgacggtcgg cctcagcgca ggcagctcta cgttgacctt gcgcccggca aacttcagct
     3601 tggtcgacga cttcagcgcg ctgaccaaga gctacggcgt agcaaaaatc cgcttgcaga
     3661 tcgggggcgg gccatacaac ctgcaaacgt gatccgcttg tcatcgaagg cacgctatat
     3721 gcgcattctc gccgcggcgg tcataattcc tgccatcgca ggcgcagtgg cgtggggctt
     3781 catcgagagc cgccagggac gggaggtcga gacgaagcgg gaacgtgccg tcaaggcgcc
     3841 gctcagggta tcgaacgcga atggcgcagc tgtgatcaaa gtcgatgccg aggagcagga
     3901 acgcaacggt atccagacca ccgcgctcct gccggcgccg taccaggaag aggttcgcgc
     3961 ctatggcatg gttctcgatc tcgcacgcct caccgatctc agcaacaact acgccaacgc
     4021 gaagtctcag cttgagaccg cgcaggcaaa ggtcgccgca tcgaaagcag cattcgagcg
     4081 cgcgcagaat ttttacaagg acgggaagac catctctctt gcgcaattcc aggcggccga
     4141 ggccgctttc cgcgtcgacg acgctgcggt tggtgccgcg cagtcgcgag tgcgcactct
     4201 aagggcgacg gcggagcagg agtgggggcc ggccctcggc aaatcactca tcgatgggtc
     4261 gcgaatagtc acgcgcttga tcgaacgcca agacttcctt ttgcaagtca ccttgccacc
     4321 tagcgtctcc atcccgacgc ctcccgtcac cgcatctatt gaaactggta agggtgaacg
     4381 cgctgcaatc acgttcatct cccccgcgac gcgcactgac ccgaaaatcc aggggatcag
     4441 tttcttctac atcgcgtcgg cagagagcgg cgtcctgccc ggcatgaacg tcttggccct
     4501 gttgccgacg ggcaccaccg tgggcggcgt tacagtgtcg gcgccggcga tcgtctggtg
     4561 gcaggaccgt gcctgggcgt atcgccgcgc tggtcccaac acgttcacgc gcacagagat
     4621 ttcgaccagt ctgcccgcac ccggaggcgg ctatatcgtc aaagacctgc cgacgaattc
     4681 cgagatcgtg acgcagggtg ctcaactgct gctgtctgag gagtttcgcg ctcagatcca
     4741 ggtcggcgga gactagaagt gagtcctacc ttgccgtcag gaccgcaggc tggattgatc
     4801 acattcgcga tccgcttccg cgggatcgtc atcgcgcttg cctgcatgct acttgcatac
     4861 ggtgggtatt cgctcggccg ggtcaactac gacgtgtttc cggagttcgc accgccgcag
     4921 gtcggtatcc aaaccgaagc gcccgggctc acgccggagc aggtcgagat cctcgttact
     4981 cgcccgattg agaacgccat caatggcgta tctggcgtgc aaaaactgcg ctcgacctcg
     5041 attcagggcc tctcagtcgt cactgtcttc ttcgatcctt cgagtgacat ctaccgcgac
     5101 cgccaggtgg tggccgagcg gctggctgtt gccgcccacc aactcccgct aggggtacag
     5161 ccgcctgcca tgaccccact cacctcgtca acgagcaccg tgcttgtgat cgggctcacg
     5221 tcacaagcgc aatcgctaat gaagctgcgc acaattgctg actggacggt gcgtccgcga
     5281 ttgctcgccg tcccgggcgt cgcaaaggtg gtgacctttg gcggccacag tcgctccatc
     5341 caggtccagg tccacccgga cgaggtcatc cggtataact tgagcctgaa cgatgtcctg
     5401 gcagcagcgc gccgcgcgac cggcgtgcgc ggtgccggct tcctcgacac gaagaaccag
     5461 cgtgtggtct tccagaccga gggccaatca ctcgcggtag acgacatcgc gcgaaccgtg
     5521 ctgctcagcc agggagcggc gagcgttaca cttgggaatg tcgctgaggt ggtcgaggcg
     5581 ccggagccac ccatcggcgg cgccgcgatt gacggccagc tcggcgtaat aatcaacgta
     5641 tcggcgcaat atggcgccaa cacagtcgca gtgaccaacg ccgtcgaggc tgcgttgaac
     5701 gagttgcgcc caggcctcga ggccgagggc atcgtgttgc atgccgatct cttccgcccg
     5761 gccaacttca ttaacaccgc aacaagcaac gtgcagcaat ccttgattct cggggccggc
     5821 gtcgtgatcg ttgtactgtt cctttttctc ttcgacctgc ggagcgccgc gatttcctgc
     5881 agcgcaatcc cgctctcgct cctgactgca ataatcgtcc ttgagaggtt aggcgccacg
     5941 ctcaacacga tgacgttggg cgggctcgcg atctcgatcg gtgtggtagt cgatgacgct
     6001 gttatcgacg tcgagaatat cgctcggcgc ctgcgcgaga acggccgact gccgcagccg
     6061 cgaccgatcc cgcgcgtagt gctggacgcc tgcctcgaag tcaggagtgc cgtcgtctac
     6121 gccaccttcg ccgttatcct agtcgtgctc ccagtcatgg ccttatccgg tatcgccggc
     6181 cggctcttcg cgccactcgg cctttcctac tcgctcgcag tgatggcgtc gctcgtcgtc
     6241 gcgctcacgg tgacacccgc gctctctatg gcgctcctga aagatcggca cgcaccgaca
     6301 acggacccgc ccgtagtcag gtgggcgcgc agtggctacg agcgtcttct gcggtgcatc
     6361 gctcgtcgac cacggacagt cattgcggcc gcgttcgtgt tcactgtcgc cggctgctcg
     6421 gggctgccgt ttttcggtgg cagtttcatc cccgagctca aggaagatca ttacaccctt
     6481 cacatgtcgg cggtgccggg cacctcaatc gaggagtcgc tgcgtatcgg catacgcgtc
     6541 gccgacgcgc tcaagcggct gccatcggtg cgctccgttg cgcagcgcgt cggacgcgcg
     6601 gagagagcgg aagacacgtg gggcactcac tacagcgaat tcgagatcga tctcaagcct
     6661 ttgagcggcg atgagacgga aactgctgaa gcagagatcc ggaggacgct tgcagcgttc
     6721 gtcggtgtga acttctcgct caagactttt ctcaccgagc gcgtcgagga gacgctttcg
     6781 ggctataccg catcggttgc tgttaatgtc ttcggtaacg atctcgacgt tctcgatcag
     6841 aaggcgcggg atatcgccat cgagctcggc gaggtaccag gcgccaccga agttcagatc
     6901 caggcgccac ccggcttacc gcagctcacc atccgcctcc gcaaaaccga ccttgaacgc
     6961 tggggcttcg atgctgtcga ggtgctcgag ctgatccgtg cggcctatca gggcgagatt
     7021 gtcgggcaaa cgtatcaagg taaccaagtc ttcaatgtca ttacggtcct cgaccgcgcg
     7081 agtcgagaca gcattacaaa agtgggaggc ttgccccttc gcagccaagg cggcactttc
     7141 gttctcctga gacaggtcgc cgacatttac caagccgctg ggcgttacca agtgctgcac
     7201 gaaggggctc agcgcgtcca gacggtgacc gcgaatgttg ccggcgacga tgttgtctcc
     7261 ttcgtccagg cggcgaagcc gaaaatcgct accgaggtgg agcttcctgc tgggacctac
     7321 ctccagtttg cgggcgcggc tgaggcacag tcgcaatcac gacgtgacct catcatcaac
     7381 tcgctcatcg caagcgccgg catcatcttg ttgctctcga ttgcgacctg caactggcgc
     7441 aatctcaccc tgattcttgc gaacctgcca ttcgccctgg tgggcggcgt actcgccgtg
     7501 ttcgtgaccg gtggacaatt gtccttgggc ggcatggtgg gcttcgtcac tctcttcggc
     7561 atcacactcc gtaattcggt tctcatgatc gcccactacg agcatctcgt cgcagttgag
     7621 aacatgtcct gggggttgga tactgcaatc cgaggagccg cggatcggct tagtcctatt
     7681 ctcatgacct cgatcgtcac cgccctcggc gtgttgccgc ttgcaatcgg catgggcgat
     7741 cccggacgcg agatcgaggg tcgcatggcg gtcgttatcc tcggcggcct gttgagctcg
     7801 atggcgttga acctgcttgt gctgccaact ctctcgctgc gctacggaaa gtttgagcca
     7861 tcacgcgacg aattcgagca ggcgggagcg ccatcggtga gatcggtcgc cgagtaacga
     7921 taagtggact cgtgatcgat ggtctgcgac gggccttcga aaaccgggga ggatgatgac
     7981 agatgcaacg tttgcaaacc cgggcagtgt cgtcatctga tactgacacc atcaggacta
     8041 tcggaatcct gcatttcacc atcggggtac atgaccattg ccgacgcgaa gttcattcag
     8101 aagtgctcgg ctgtaggcac atgggctcga acgagcgcta ttccctcatg caacgtggcg
     8161 gcagctactt cgtgttggcg aagatccgcc acaccctatt gccgagctac acatcgtggc
     8221 gaccatctcc tcggcgcgct aaaggtcgta gtcggcggtg aggactgcgt cggtcattcg
     8281 attacgagag caccggcgca gagacacgag gccgatcaac accgcgttca ccactgggtc
     8341 cacgttcaga tacatgcccc aggggctgag atggtgcggc agcctcgcgg tcgcgaggac
     8401 attgtcctgc gcgagcgccg ctaaaggttc gtcgagccat gcgatcggct gggacgtgtc
     8461 cttataagcc cggtttctgt aagcgcggct cagtcaatgt ccgcttcgcg ccggaagcga
     8521 ccgaagtgct gcgttgccgc tcgacgtagt cccgagaacc ggtcattcgg cagtcgctcg
     8581 cccagtcccg ccgatgctga ttcggatgca aacagcttgc tggcacgcgc gatcgcctca
     8641 tgtcaggcga atttcaccgg agattgaagc ggatcggcac ggtcagatcc acacggtcgc
     8701 ccggcaattc tcgcggcggc gccggaaagg gttgcgcgcg gcgcaacagc gccagggcct
     8761 cctcgtcgag cgcgctcgcg cccgacgagc gcgagatacg gctgtcgatc acacgccctt
     8821 tccggtcgag gctgaagaag acctgggcga tccgttgctc gcgacgagat tgtgctaccg
     8881 ccggatagcg cttgttacgc tcgagcagcg ccacgatctg cgttttccac gtcggtaccg
     8941 cgttcgaatt gtgcggggtg atccggccct gtatgggggc ggcaggaatc gcggcggtct
     9001 gatctggaag cgcctgcggc gcggaggtcg tcggtgccgg ggggcgcggc tcttggcgct
     9061 gcggggttgg gacctcctgg ggtgggggct caactgcaac gtccggattg ggggcgggct
     9121 tgatctcggg cggcggctct tcgaccggct tcgactcgac cttttcttcg actttctcct
     9181 tttcctcgag actcttgacc ggcttgttgg gcgaggcgtc ggacatcacc tgctcgggtc
     9241 cggggggaat ctccgtctgc ggcgcagccg gcgcgaccgg catcggggcg aactcgatca
     9301 cgatcgcggc agcgggctct gccggctcga tctcgtcgcg ccaggtgacc atcccggcgg
     9361 cgatggcgcc gtgcgcgaac acgatgacgg cgccgcacat cacccaccgg cgaaggtccg
     9421 cacggtcttc ggccataaac ggcgtgacgt taatctcatg catttcgtca agcgtgtcct
     9481 cgccgtggtc gagacgggcg ctcatgacga cacactcaag tacgctcgac agcggacaat
     9541 ggcggcgccc tccgcgcgga cggagcaggc acgacgacgc gctcgaggtc gcggctgacg
     9601 agccccagga cctgcgccgc ggcatcgcca accccggcgc ggtagcccgc gatcgaccgc
     9661 gcgaacacgt tgtagatcac cacggccgga atggcggccg caagcccgaa tgcggtcgcc
     9721 agcagcgctt cggcgatgcc cggcgcgacg accgcgaggt tggtggtatg cgacttcgag
     9781 atgccgatga agctgttcat gatgccccag acggtaccga acaggccgac gaagggggcg
     9841 gtcgcaccaa tggtggcaag cacgccggtg ccgcgcaaga tccgccgccc gtagcttgct
     9901 tcgatgcgct cgagacgcga cgcgatccgc tccttgatgc cttctcggtc gatggaaccg
     9961 gatgagacct ttaactcggt cacggcggcg tcgaggaact gcatgacctc gccccgagaa
    10021 tcggccagac gctccacgcc gtccgccgtg gagcgtacgt ggacaagctt attcaatgcc
    10081 tcccgcacac gtcgcttggc gaaaaagatc tcaatggttt tggcgagcca cactgtccaa
    10141 gtgacgattg aagcaaaggc aaggccgatc aaaaccgcct tcacaaccag gtcggcgttg
    10201 agatacatgc cccaggggct cagatcgcgc ggcagagtgg cgatggccac gacgttatcc
    10261 tgcgcgagcg ccggcaaggg cacgatcagt aatgcgatca tggcggcacc gcgcagcaac
    10321 gcgagggcaa caaatgccgg atgggttcaa atccgaccaa ggcaaagtta cgaatccgcc
    10381 gggaggaggc gcacagcgac gcgaatcagc tccggcagat ttttggcgcc aagcgtccgc
    10441 ttaagatgga ggcaggtatt gaccacggtc ttgtagctga cgcctagctc ctcggcgata
    10501 ctgctataag ttttgccctt cgccaatagc gagagtatct gtgattggcg tggggttaga
    10561 tcgacgagcg ggttctccac gccggtgcgc aagagcgcga ctcgcatcgc gagatcttcg
    10621 ctgagatagg gcttacccga gcgcacctgc tcgaacgcct tcatcagctc gtctgaggac
    10681 ctatccttga gcaaatagcc ggtagcgccc gcttcaagag cgcgcgcggc gatcatcggg
    10741 tcgttatgca tgctgaacac aaggatgcaa gtgcacggct cgtgcgatcg catgcgccga
    10801 atgaggtcca ggcccccaag cacgctgccc tgcatgccga gatcgacgac gatcacgtcg
    10861 ggacggttgc gacggaaaag ccggtagccg gaggtcacgt cgcgtgcctc cagcacggga
    10921 ttgacgccgg cgtcttccag cacgcgccgc aacccctgca acacgatagg atggtcgtca
    10981 atgatcagaa cgctcgtcat actggcctat cttcatacga aaaaatactc tggaggtcgt
    11041 gcacgcgtcg cgatccgtct tttgccgcga ctgcgcgcgc attctccaag atcatccacg
    11101 ttgcttcgtt cgcgagttct acatttatga gatgccaaga gccagataga gcacctgctc
    11161 aatgtccttt gaggcgctcc ctaagatgtg ggccgctcgc gcggggcgtg gcagcgtggc
    11221 cagcagtaag tagcgcagat cggctgggtc agccattcga gccacgacgg atgtagcttc
    11281 tgttgcctta cacttagggt tggtaaaata gtggccaaaa agtcacgatg acataacttc
    11341 gtatttcagg ttcgcgacgt tctgccagat aatttggcaa t
//
LOCUS       DATLJT010001069         5611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1222997,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001069 DATLJT010000000
VERSION     DATLJT010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222997"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VM910_24535"
     CDS             complement(<1..315)
                     /locus_tag="VM910_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65723.1"
                     /translation="MSRRAFITLLGGAAAWPLAARAQQADRGRRIGVLMGTAMNELRI
                     VACVAGLLAAGGSYAQTYPSGNVRVVVPYPAGGPTDVMARLVAQKLSEALGQQFYVEN
                     QGG"
     gene            complement(312..1289)
                     /locus_tag="VM910_24540"
     CDS             complement(312..1289)
                     /locus_tag="VM910_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65724.1"
                     /translation="MIAPIKRREFITLLGGAATWPLAARAQQPAMPVVGYLNLQSFDG
                     FNWPLRAFRQGLKEGGYVEGENVAIEYRWAEGQLDRLPTLAADLVRRQVAVIAATGGP
                     PAVLAAKAATTTIPIVFITGDDPVRLGFVTNLARPDGNLTGISFFNAELAAKRLEFLR
                     ELVPGAARVAVLVNPANAAIAETTLRDVEAGARAMALQIQVLNASTSREISAAFATLG
                     RARPDALFVGPGAFFVTRRVQLAHLATRHAVPAIYAEREYVEVGGLVSYGASLTDAYR
                     QVGVYTGRALKGGKPADLPVELVINAETARILGLTVPDKLLVAADEVIE"
     gene            complement(1286..2278)
                     /locus_tag="VM910_24545"
     CDS             complement(1286..2278)
                     /locus_tag="VM910_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65725.1"
                     /translation="MSGVGRREFVTLLSGAAAAWPLAARAQQPSTPVIGFLNAASPGP
                     LRQQIAAFHEGLKESGYVEGQNVAVEYRWAEGQYDRLPALAADLVPRQVSVIVSGGGA
                     PVVFAVKAATTTIPIVFTVGGDPVGLGLVASLNRPGANIAGVYHFATGLEAKRLGLLH
                     EMVPKAATIAVLVNPNYSDAENQLREVQEAAARLGVQLVVVRANAESDFDAAFSTLVQ
                     QRSGALLVCSSPFFNGRREQLVVLAARHALPAIYEWRDFCAAGGLISYGTSLADAYRQ
                     AGIYAGRILKGAKPTDLPVLQSTKFEFVINLQTARSLGIEVPAGLLSIADEVIE"
     gene            2494..3084
                     /locus_tag="VM910_24550"
     CDS             2494..3084
                     /locus_tag="VM910_24550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65726.1"
                     /translation="MRLELHYRSDVDLSRPGMQVEIRNHIARCLRDYPGGDEGVITLA
                     GVEHRFMYVVHDDVVDTVIGPPDYIEHVANERRQTAHEARSPLPRKEHNQLVLGASPV
                     SETRQNELAQEPAAVAPRGIGAGAGGLKGRCIYRYRLRLRWITSIFIRLGCICDAALL
                     DVLGESSRAKPRQPRRTLGSATRRFGFWGRATEGAD"
     gene            3346..3744
                     /locus_tag="VM910_24555"
     CDS             3346..3744
                     /locus_tag="VM910_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65727.1"
                     /translation="MSALCSDDYCEDAAGQCGAISAGEVFTIEAILAKYGIVITGDID
                     EDVIVLFLDDLEDTPVGSIQPRNEDAVSTDEKSNAEPANEQETIATKVVTVQPPLEIG
                     GEAADDVLTTDPTARRIPAAAEAVDHRAIE"
     gene            3768..4166
                     /locus_tag="VM910_24560"
     CDS             3768..4166
                     /locus_tag="VM910_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65728.1"
                     /translation="MLTVLKDENTLSAPINAKYRVMSPVSLVPEQDLHFVLCDFGRSG
                     QAYVETDPTEADASTIVRNLLRGQYDRPVRVLALNVEEGWVRDVSEIIAAKVQDVAGR
                     EEQRLTSGTRAFVEAHSEPTDEQQMLPLWQ"
     gene            4685..4897
                     /locus_tag="VM910_24565"
     CDS             4685..4897
                     /locus_tag="VM910_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65729.1"
                     /translation="MAPRHVGNGEIEALAVHQRGDEGDAAAQAIQLRHQQGGLALLGQ
                     RHRGGQLRPVAALAERQRTISSAIAL"
     gene            4894..>5611
                     /locus_tag="VM910_24570"
     CDS             4894..>5611
                     /locus_tag="VM910_24570"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HVI65730.1"
                     /translation="MIYAAEERHVLLTTEKIRALVDNIGGKKDRVTEGEVIKRTTAMA
                     DRFLHEVKRMHQLRQDINEMTPEEADELIRGLGDDAAEDWVFAARDAQLPPPELDWCW
                     LFLGGRGAGKSHSMSAAVHTAVRAGISRIHFIAPTTSDFHDVNIEGKSGIFATCGRDP
                     RPRWLSSRRRLEWPNGAMCVFFSGEEPESLRGPQCELAVIDEIGRMRYQQAVFDNMML
                     GLRLGDRPRVLLATTPRTTPF"
BASE COUNT         1063 a         1789 c         1763 g          996 t
ORIGIN      
        1 cccgccctgg ttctctacgt agaactgctg gccgagcgct tcggagagtt tttgggccac
       61 caggcgggcc atcacgtcgg tcggaccgcc ggccggatac ggcacgacga cgcgcacgtt
      121 tccgctcgga taggtttggg cgtagcttcc gcctgcggcg agaagccctg ccacgcacgc
      181 gacgatgcga agctcgttca tggcagtgcc cataagcaca ccaatccgcc gcccacggtc
      241 cgcctgctgc gcgcgcgccg cgagcggcca cgccgccgcg ccgccgagca gcgtgatgaa
      301 cgcgcggcgg ctcactcgat cacctcgtcg gcggccacca gtagcttgtc gggcacggtc
      361 aggccgagta tccgggcggt ctcggcgttg atcaccagtt cgaccggcag gtccgcgggc
      421 ttcccgccct tgagggcgcg gccggtatag acgccgacct ggcgataggc atccgtcagg
      481 ctcgccccgt agctcaccag cccgccgact tctacatatt cgcgctccgc atatatcgcg
      541 gggaccgcgt ggcgcgttgc caggtgggcc aattgcacgc ggcgggtgac gaagaaggcg
      601 ccagggccga cgaatagcgc gtccggccgc gcgcgcccaa gcgttgcgaa ggccgcactg
      661 atctcccggc tggtgctggc gttgagcacc tggatttgca gggccatggc gcgagcacct
      721 gcttccacgt ctcgcaaggt agtctcggca atcgcggcat tggccggatt gacgagcacg
      781 gccacacggg ccgctccagg caccagttca cgcaggaatt ccagccgttt tgccgccaac
      841 tcggcattga aaaaacttat tcctgtcagg ttgccgtccg gccgggcgag gttcgtaaca
      901 aaaccgagcc tgaccggatc gtcgccggtg atgaagacga tggggattgt cgtggtggcc
      961 gccttggcgg ctaacacggc agggggacct ccggtcgcgg caatcacggc gacctgtcgg
     1021 cgaaccaagt cggccgccag cgtcggcagc cgatccaatt gaccctcggc ccagcgatat
     1081 tcaatcgcca cgttctcgcc ctcgacataa ccgccctcct tcagtccctg gcggaatgcg
     1141 cgcagcggcc agttgaaccc gtcaaacgat tggaggttca gatacccgac caccggcatc
     1201 gccggctgct gcgcccgcgc cgccagcggc caagttgccg cgccgccgag cagcgtgatg
     1261 aactcgcgtc gctttatcgg tgcaatcatt cgatcacctc gtcggcgatg gagagcagcc
     1321 ctgccggcac ctcaatgccg agcgatcgcg cagtttgaag gttgatgaca aactcgaatt
     1381 tggtcgattg cagcaccggc aggtcggtgg gcttggcgcc cttgagaatg cgaccggcgt
     1441 agatgccggc ctgacgatag gcgtcggcaa ggctggttcc gtagctaatc agaccgccag
     1501 ctgcgcagaa gtcgcgccat tcatagatcg ccggcagcgc atgacgtgcc gccagcacga
     1561 caagctgctc gcgccggccg ttgaagaacg gggatgagca gaccaagagc gccccagatc
     1621 gctgttggac gagggtcgag aacgcggcat cgaaatcgct ttcagcattg gcgcgcacga
     1681 caacgagttg cacgcccagg cgggccgccg cctcctgcac ctcgcgcaac tggttttcgg
     1741 catccgaata gttcgggttg acgagcaccg cgatggtcgc agccttgggg accatttcgt
     1801 gcaatagtcc cagccgcttc gcctcaagcc ccgtggcgaa atgatagact ccggcgatgt
     1861 tggcgccggg ccgattgagg ctggcgacga ggcccaaccc gacgggatcg ccgccgacgg
     1921 taaagacaat gggaattgtc gtggttgccg ctttgaccgc gaacacaacc ggagcgcctc
     1981 cgcccgagac gatcacgctc acctgccggg ggaccagatc ggccgccagt gccggcagtc
     2041 ggtcgtactg accttccgcc caacggtatt cgaccgccac attctggcct tcgacatagc
     2101 ccgattcctt cagaccctcg tggaacgccg cgatctgctg gcgtaacggt ccgggcgatg
     2161 cggcattgag gaatccaatc accggcgtcg acggctgctg cgcgcgcgcc gcgagcggcc
     2221 acgcggctgc cgcgccgctg agcagcgtca cgaactcgcg ccgtcccacg ccggacatgg
     2281 gaggtccctc cctccgggat caccgcacct tcagcctgcc accggggcgc ccggaaggtc
     2341 catggggcac acctgaattg ttctgaatca gggtccctac cgcagtcggg aggaacctag
     2401 cccatcactg cctgtccttg gctacctcac gttcacttct tatcggcata tctatgcgat
     2461 agggagcggt ttcacggggt cggggaggac gtgatgcgcc ttgaactgca ttaccgcagc
     2521 gatgtcgacc tcagccgacc cggtatgcaa gttgagattc gcaatcacat cgcacgctgt
     2581 ctgcgcgact accccggcgg cgacgagggg gtcattactc ttgccggggt cgaacatcgg
     2641 ttcatgtacg tcgtccatga cgacgtggtc gacaccgtca tagggccgcc cgactacatc
     2701 gagcacgtcg caaacgagcg tcggcaaacc gcgcatgaag cgcgatcccc gctgccgcgc
     2761 aaggagcaca accaactcgt actcggggca tcaccggtct ccgaaacccg gcagaacgag
     2821 cttgctcagg agcccgccgc tgtcgcgccg cgagggatcg gcgctggggc gggcggctta
     2881 aagggtcgtt gcatttacag gtatcgtctt cgtctccgtt ggatcacaag cattttcatc
     2941 agactggggt gcatttgcga cgcggcgctg ctcgacgtgc tcggcgaaag ttcacgagcc
     3001 aagcctcgcc agccccgccg tacattggga tcggcgaccc gccgcttcgg tttttggggc
     3061 cgagcgactg aaggcgcgga ttgaactttt ttgggtgacg ggtcgccctg ctgcgcagcg
     3121 atgagcagat cgccaagcgc gcgaaatgag ttggccgggc cgcagttaac cagacgaaag
     3181 aatacagatt ccttcaaagc tcaattgtaa acgcctctta aacccacctg agagagcctg
     3241 ccgccttcga aaaaggaggc tttcccgtga aacttctcta cgccatcgct tctacactgt
     3301 tttgcgtagc cgctccggcg gccgcttccg cacaatcgct cgactatgtc ggcgctgtgt
     3361 tccgacgact actgtgagga cgcggctggc cagtgcggtg caatctctgc cggggaagtc
     3421 ttcacgattg aggccatctt agcgaagtat gggatcgtca tcaccggaga tatcgatgaa
     3481 gacgtaatcg tcctcttctt agacgacctg gaggacacgc cggtcggttc gattcagccc
     3541 cggaacgaag acgcggtctc gaccgacgag aaatccaacg ccgagcctgc caacgagcag
     3601 gagacaatcg ctactaaggt cgtcaccgtg caaccgccac tcgaaatagg tggcgaggcg
     3661 gcggacgatg ttttaacaac cgacccaacc gcgcgccgca tcccggcggc cgccgaggct
     3721 gtcgaccatc gggcgatcga atgaagtcgt gacaagggct tggcctgatg ttgacggtcc
     3781 tcaaagacga gaacacactg tcggccccaa tcaacgcaaa gtatcgcgtc atgagccccg
     3841 tatcccttgt gcccgaacaa gacctgcact tcgtcctttg tgacttcggt cggtccggtc
     3901 aggcatatgt cgagactgac ccaacggaag cagatgcctc gaccatcgtg cgcaatctcc
     3961 tgcgggggca atacgatcgg ccggtgcgag ttttggcact gaatgttgaa gaaggttggg
     4021 tgcgcgacgt gtccgaaatc atcgccgcta aggtgcaaga cgtggccggg cgtgaggaac
     4081 agaggctgac ctcgggaaca cgtgcgttcg tggaggcgca cagcgagccg actgacgagc
     4141 aacaaatgct gcccctatgg cagtgaaatc gtagagatca ggcccgcttc attgctgctc
     4201 agaccttcgc accacgcacg ccggatgagg gatgctctcc cgttggagag cgactacatc
     4261 cacgccgagg cccgaagcct ctaccgccat ctcccgtgtg gcctctcgcc gagctccggc
     4321 ccggtggaaa aaagttcacg ccggccaaaa acaacccccc gggtcatcac aggccgagac
     4381 gatggcggac gcgacgaatc tgcatcggaa accattggcc gccattcggt gccgcgatgc
     4441 cgcgcgcgtt gagatcggtt gcgatggcac tgctcgatgt gcggccagcc ttgatgcagc
     4501 gctcgatgtg tgggcgcagc gctcgagccc gctcggccgc gttcgccgat tgatcttggc
     4561 ctgctccgga ttgccgagct tgacgccccg tgccttcgcg gcggcaagtg catggcgtgt
     4621 gcgctcgccg atcatgtggc gctggtgctc ggcagcgggg gcgagcacgt gaaggatcgg
     4681 ccggttggcc ccccggcatg tcggcaatgg cgagatcgaa gcccttgctg tccatcagcg
     4741 tggcgatgaa ggcgacgctg cggctcaagc gatccagctt cgccaccagc agggtggcct
     4801 tgcgcttctt ggccagcgcc atcgcggcgg ccagttgcgg ccggtcgctg cgcttgccga
     4861 acggcagcgg acgatatcgt cggcgatcgc gctgtgatct atgccgccga ggaacgccac
     4921 gtgttgctga cgaccgagaa aatccgcgcg ctcgtcgata atataggagg caagaaagat
     4981 cgcgtcaccg aaggcgaagt cataaaaagg acgacggcaa tggctgatcg ctttttgcat
     5041 gaagttaagc gcatgcacca gttgcgccag gacatcaacg agatgacacc ggaggaagcc
     5101 gatgagctga tacgaggact aggcgatgac gcggccgaag attgggtctt tgccgctcgg
     5161 gatgcacaat tgccaccgcc cgagctcgac tggtgttggt tatttctcgg cggccgcggc
     5221 gccggcaaat cgcactcaat gtcggcggcg gtgcataccg cggtgcgcgc tggtatatcg
     5281 cgcatccact tcatcgctcc aacaacgtcc gacttccatg atgtcaacat cgagggcaag
     5341 agcggcatct tcgcgacctg cggccgtgat ccgcggccgc gctggttgtc atcgcggcga
     5401 cggctggaat ggccaaacgg cgcaatgtgc gtgttcttct ccggcgagga gccggaaagc
     5461 ctgcgcggtc cgcaatgcga gcttgccgtg atcgacgaaa tcggccgcat gcgctaccag
     5521 caagcggtgt tcgacaacat gatgctgggc ttgcggctcg gcgatcggcc gcgcgttttg
     5581 ttggcaacta caccgcgcac aaccccgttc a
//
LOCUS       DATLJT010001070        15069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3980246,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001070 DATLJT010000000
VERSION     DATLJT010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3980246"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VM910_24575"
     CDS             <1..877
                     /locus_tag="VM910_24575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65731.1"
                     /translation="KMIVPFPPGGPIDTMARLVAHELSTRLAQEVVVENRPGAGSTLG
                     SKAAASAEADGYTLLFGSSGSLAVAPALYAKLNIDPLKLFAPVASVSLLPHVFVVAPS
                     VPAKSVQEFVAYAKANPGKLNYGAGIGTPPHLLSTLLKVKAGIDVTYIPYKGSAASIT
                     DLLAGQTHFTIDGLLGLYPLVNEGKVRALGIARAERWPALPTVPTLVESGFPDFTHDA
                     WTGVVAPAGTPRDVVDTLNRAINDGLQSAEMKENLIRFSAIAKAGTPQDFAAFMRSEL
                     PKWAELVKLAGATAE"
     gene            958..2019
                     /gene="hemB"
                     /locus_tag="VM910_24580"
     CDS             958..2019
                     /gene="hemB"
                     /locus_tag="VM910_24580"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HVI65732.1"
                     /translation="MAIKYGRPIEARLAPVEAKIKRKPRLDLAKRPRRNRKAEWARRL
                     VREHALTAHDLIWPLFLTDGSKTRVPVESMPGVERLSVDEAVREAERAAELAIPCLAL
                     FPYTDPKRRDEDGSEALNPDNLVCRAIRAIKQAVPEVGVLCDVALDPYTSHGHDGLLR
                     DGVILNDETVEILVQQALVEAEAGCDIIAPSDMMDGRVAAIRAGLDRAGFADVSIMAY
                     AAKYASAFYGPFRDAVGSAATLVGDKRTYQMDPANTDEALREVELDIAEGADMVMVKP
                     GMPYLDILRRVKDAFGVPTFAYQVSGEYSMIMAAAQNGWLDGEKAMMESLIAFKCAGA
                     CGVLSYFAPRVAEALKSAS"
     gene            2209..2373
                     /locus_tag="VM910_24585"
                     /pseudo
     CDS             2209..2373
                     /locus_tag="VM910_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
     gene            2505..3716
                     /locus_tag="VM910_24590"
     CDS             2505..3716
                     /locus_tag="VM910_24590"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI65733.1"
                     /translation="MTTRCKLDSKTARLKLPVRKKPYTNRAAPGIRIAYRRNEGPGTW
                     SVLGGGGLWLKKIGIADDLEPADGVNVLDYWQASDRARDLARARDANSGKSVTVVEAL
                     ATYRDDLQSRGGDPYNADRVLAHLPKALLSKAVGLLRATELRRWRDALLAQGLARGTV
                     GRTIRMAKAAFTAAADADTRIANRNAWQVGLKGLPDAENARNVILPDAIVRKITVAAR
                     PEGPEFAVLVELAAVTGARVSQLRRLQVADVQANRSDPRVMLPSSAKGRRKVSTRRPV
                     PIPAALALRLRQLGAGRSAHAPLLLKADGNAWGRSDHLRPFQRTVARAGLDPGVTIYA
                     LRHSSIVRALLGAVPARVCAANHDTSVVQLERTYSKYIADHSDAVARHALLDLASPAG
                     AEVVPLSGRRA"
     gene            3713..4348
                     /locus_tag="VM910_24595"
     CDS             3713..4348
                     /locus_tag="VM910_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65734.1"
                     /translation="MSKKAAKRTKAATALPLVSPHWWSREQTVAYCRAHKGEIADGAL
                     TAAVNAGDLPVKLEWIDHQTSSPVQHKLLLSAEDYAFGPYISSNFLVIQPRRPDVPPL
                     PRPHALFFWGPKAKELWPAQEAEPVTTKQQAADPFKPPRRRPGPPPENEWKQAVVREL
                     LRRAKAGKPLPSAPDMQEFCQDVLEWEPDLRSMQELLRLFAGEMWHLLGLG"
     gene            4437..4781
                     /locus_tag="VM910_24600"
     CDS             4437..4781
                     /locus_tag="VM910_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65735.1"
                     /translation="MVDISENRPVDPAVEAAARGRVVSKRDRRVKVKQKTHDRPQART
                     PPAPLGAAICAFSIADFCRAHGGMSQAFFHALVAKGKGPRLMKVGARTLISVEAAAKW
                     RRAQEAATDQVN"
     gene            4924..5100
                     /locus_tag="VM910_24605"
     CDS             4924..5100
                     /locus_tag="VM910_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65736.1"
                     /translation="MSHIQIRNQKRKPRRLLYRDGITRDELMRLVLEVGRDQVEGVLK
                     ALDLPTEPALTPAE"
     gene            5186..5371
                     /locus_tag="VM910_24610"
     CDS             5186..5371
                     /locus_tag="VM910_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65737.1"
                     /translation="MNNILADRILVDLIQQLDAVRELHEQANTADALLHAQTLVDDAL
                     RQATNFLFELRRKQLGI"
     gene            complement(5386..5769)
                     /locus_tag="VM910_24615"
     CDS             complement(5386..5769)
                     /locus_tag="VM910_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65738.1"
                     /translation="MERALTRTFLVAAAEMVGLFPDRRLDDRDRDLATGDRRLYGTRR
                     SSIFEPRRCTLPGHRLRALDKSVLRPMLLNPGTDRENVHPLFRFNTDHWNIDRVHKHG
                     CAAVADVDEGKTAMNAGIDADHAHE"
     gene            5859..6350
                     /locus_tag="VM910_24620"
     CDS             5859..6350
                     /locus_tag="VM910_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65739.1"
                     /translation="MKPFIDITGERFGRWERTMSGAHRQNDEYGGQVMNKEVERIRVE
                     RELRSMVEERLFGDGPWSPDDPKVNRAISDKLSALGLQHVEPVTGAVHTTELGRELNV
                     DTLTLFTGLHEPYEVPDCLVPLITQAEANELRERFDNGERPEEVLPPLLRQLWRKHYQ
                     AKH"
     gene            6368..6607
                     /locus_tag="VM910_24625"
     CDS             6368..6607
                     /locus_tag="VM910_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65740.1"
                     /translation="MEIPTKYADKTVLKACAALMMSVDNDEDFDTGDLIRRLAETLAP
                     WAARVACDDHLALARLVAEAMTLQFYCDRGELLPE"
     gene            6626..8116
                     /locus_tag="VM910_24630"
     CDS             6626..8116
                     /locus_tag="VM910_24630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI65741.1"
                     /translation="MNAPFGMDRQDFLTQAHFSGNRRLLPHQITQAIFAVERARSLNA
                     SETGTGKFLVALAMRRLIEREAGRNVKCLYTCPKSALGQFEQEFIDHGYRTFVLRHGN
                     DVVPVDVDTVLVANSTMVVTHRDQLRSWRPLLVVLDEAVAFKTATAARTKAVYGDALD
                     GAGGIIDEVPFVLAMSGTLAPAHNGELYPHLRALAPAALLDERGRIMRRHVFEKTFCV
                     FGARRIAGNREVQVIVGSRNSNLLRKRIDPYVAHVTLREIAPTLPPERHERVPIARED
                     VKLDELAAITDIEDAEIRSAVEMLATAIRDGLVPAPDIDREMARLMQMIGGGTALAHL
                     RRAYGLAKLPYVEDVVMSRRGGSREERTPTLIFNTYRLTGDRLETLLSEQDVMVGRIH
                     GNTSAAERHAVVSGIQDGRVEAAILQIDAAGSALNLQTANRIIMLEPSWTPGANHQAV
                     ARAVRIGQCNPVLISWPTVRHSIDEAVIRVLRRKQDGLTELWRAAS"
     gene            8113..9078
                     /locus_tag="VM910_24635"
     CDS             8113..9078
                     /locus_tag="VM910_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65742.1"
                     /translation="MIRLDALREMIIEARHNEATVRPFPSGVAAGDNRTGFLNASEAG
                     RCTRWLWYDKHDVAGEARHPYGVFDRGHAFEHWCTTYLSAGLARAEGRLLYAGAKQRR
                     LMLPDFRLAGTPDGLIWWRDQSETVLEVKSHGTAQNYDSCPSEMHVRQAELNIELFHE
                     VTAHRPEDAIIIYGLAEDYERLILHRVERRPAIFIEMLGKAAAAFDADNATAVTAEGV
                     VTKQCVICPYRSRCAAAQAHAIAAAVTGGLDQATLRSIDRLIAARARAVEAEEEAAHA
                     RAEVEAEIIATLTDGGASKVKRNGYAVQLKTPTDGLAPFLEIRSA"
     gene            9171..9905
                     /locus_tag="VM910_24640"
     CDS             9171..9905
                     /locus_tag="VM910_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65743.1"
                     /translation="MQLPALKNAVPASLIDGQSIEDFIRNSTQNLQRMSAGMPGSGIA
                     GAAYLGFNGTSGAWKLDREEVDPKSLGRILVPQHGLFEGCIEWANSSPLQKIQRQLLG
                     IHYDEPMSERLLPKPLSPNAYKKETDGPSLTLGFLGFMLDDGANVVFEHSSMGAKKAV
                     SALATTATQALIAFGEIVHPVIELGSGSYENSYRTIYDPKLNVIGYVTDKRAREVNVL
                     SEDDIITRPTASRARLRRETKEAPAL"
     gene            9963..14303
                     /locus_tag="VM910_24645"
     CDS             9963..14303
                     /locus_tag="VM910_24645"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI65744.1"
                     /translation="MDVRDITAENVAKAVGGTVERNGSILCRCPVHEASGIHNPSLVL
                     SITEAQRIIFHCRSQNCDAKHFQAIRDHLVEKCGLPRSHVGGNSRAQGEFHYHYVNLD
                     GSYSWTKIKRVTRTGKKTFVSAVYDKGTDQWTFSRPKGVPLLFNLAAVGQVLAAYPTT
                     PLLLLEGEKDVQTAGNLGVLATSCADGAGSWRIEDTRKLIELGVKKVIVCPDNDGPGI
                     NHGIEIAKSFQQAKIEVCWLSLPAKDLSEWEPKQAYPDALLKELMDAAPPFDADALDW
                     CSRLKRARPNAGCSYRGDLPNALLALQFEPRLKGCFAWNDFRHRVEVVRKTPWCLPDW
                     WEAAALTPVGHRALCDADMAELSSYLTLTYDFGACAVSTCRHALHTEAKRHSFDELKD
                     RLDGLPGWDGRHRLNGWLVTYAGADAQAHTKEYLELIGSKFIMQVLHRALNPGAKADY
                     SVVFVSIQGTGKDLVFDAMFSPYYREGIPSPRVSQADFALALAGGIVAHAAEMSAWRK
                     ADVEDQKAALTRCVDHGRRAYGYEACTYPRRTCLAFSTNDVECLADTTGDRRYWNVCI
                     ERGRIDIEALRRDRDQLLAEALHRLKEGEQHWPTPEEEERWIVPERQKNMPAAALEIL
                     AILERFIVEEPQTTRPNRGDFAWKWEPRPQPLSELYVDAFFEKCFGMYAGVRRQGLDR
                     ASKRDVDYCTTYLREHDWRRVRRLLPDGQKVQIWCAPTPSGGGVFRGHPSSELGCPQT
                     KDVELASQDAQQNCADAPDLAVTTTVDTQRTPKIARWVSTRNPLRSSTSSVLDTQPPK
                     TLLNDVFRSIFDSGGETQFTFEIALGKILGCPRALDGLLPVTLDSLEEHFPRDRFLSL
                     DVETTGLSAGCDGVRTVQIADGESAAMVVFDRPVAARALVVLADFLRGRRVVAHNARF
                     EASWLQQAGIDLVLDDTVLLFSAVRGTRSPKGDKYIGGGRVSLAALAVMVLGETLDKS
                     EQVSDWAAPTLSASQLTYALNDAIVTHRIWEALRAELHCKSKQHGVDIVAGYEDMRFS
                     AAMAHRMECVGIGFDVAAHQAWIARKQQPVAVLEAHLATLDPALSSACIASGVQLDRL
                     FRQRLELYAAQDRRPALLAWPKTEKARRLSFGREDLAAVLTANRLQGAERQLVEALYT
                     RAEQVRCLATFGTAFSSHVIDGRLHGQLHAGGTVTGRYTSSNPNLQNIPTDLEFRGFF
                     CAPAGRAFVDVDYSQLELRVFAALSGDAKMIAAFEAGWDYHDLVMQRLGCTRRQAKAV
                     NFGIIFGMGAATLAADLGIDEVTAGEYLRGWDEQAPIGAEWRASLPRLYVAEQGVRTA
                     RRWIDYLDDDDADVAANTRPKNYPVQGGAADVMHRAMRLLFEHYRDWPGNVLPVLTVH
                     DEVLVEVNIAVADRVGSLLADVMVEAFRDVLPNGPMRFLAIPGVGPTWAAAKADGEMR
                     EKALRTARCA"
     gene            complement(14847..>15069)
                     /locus_tag="VM910_24650"
     CDS             complement(14847..>15069)
                     /locus_tag="VM910_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65745.1"
                     /translation="ASKVLNGLADPGPEAAAEQEKIYIKIDLGADVRAKGMESSPADV
                     LIIEAAPRPSTPKQIEDDHSESPSDEWRR"
BASE COUNT         2792 a         4688 c         4761 g         2828 t
ORIGIN      
        1 caagatgatc gtgccgtttc cgccgggcgg cccgatcgac accatggccc gcctggttgc
       61 gcacgaattg tcgaccaggc tcgcccagga ggtcgtggtc gaaaatcggc ctggtgcggg
      121 ttcgacgctc ggttcgaagg ccgccgcaag cgccgaggcg gatggctaca cgctgctgtt
      181 cggatcgtcc ggctcgctcg ccgtcgcgcc cgcgctctac gccaagctca acatcgaccc
      241 gctcaagttg ttcgctccgg tcgcttcggt ctcgctgttg ccgcacgtct tcgtggtggc
      301 gccgtccgtg ccggcgaaat ccgtgcaaga attcgtcgca tacgccaagg ccaatccggg
      361 caagcttaat tacggcgccg gcatcggtac cccgccgcat ctcctgagca cgctgctcaa
      421 ggtgaaggcc ggcatcgatg tcacttacat tccctacaag ggctcggcgg cctccatcac
      481 cgatctcctg gccggacaga cgcatttcac catcgatggg ctgcttggac tctatccgct
      541 cgtcaacgag ggtaaggtgc gagcgctggg cattgcgcgc gccgagcgat ggccggcact
      601 gccgacggtg ccaaccctcg ttgaaagcgg atttcccgat ttcacgcacg acgcctggac
      661 cggcgtggtc gcgcccgccg gcacaccgcg tgacgtggtc gacaccctca atcgcgccat
      721 caatgacggc ctgcagtcgg ctgaaatgaa ggaaaatttg atccgcttca gcgccatcgc
      781 aaaggccggc acgccgcagg atttcgccgc cttcatgcga agcgagctgc cgaaatgggc
      841 ggagctggtt aaactcgcgg gcgcgacggc ggaatgatac ctcgccgcgg cgagagcccc
      901 gctggcgggg ctgtcgcaga ggggctaaaa agagccgtct tcatccggag cgcccccatg
      961 gccatcaaat acggccgtcc gatcgaagcc cgtctcgccc cggtcgaggc caagatcaag
     1021 cgcaagcccc gactcgatct cgccaagcgc ccgcgccgca accgcaaggc ggagtgggcg
     1081 cgccgcctag tgcgcgagca cgccttgacc gcgcatgacc tgatctggcc gcttttcctc
     1141 accgatgggt cgaagacgcg cgtgccggtt gagtcgatgc caggcgtcga gcggctctcg
     1201 gtcgacgagg ccgtgcgcga ggccgagcgc gccgccgagc tcgccattcc atgcctcgcg
     1261 ctttttccct ataccgaccc caagcggcgc gacgaggacg gcagcgaggc gctcaatccc
     1321 gacaatctgg tgtgtcgtgc cataagagcc atcaagcaag cggtgccgga ggtcggggtg
     1381 ctctgtgacg tcgccctcga tccctatacc agccacggcc atgacggctt gttgcgcgac
     1441 ggcgtcatac tcaacgacga gaccgtggag atcctggtgc agcaggcgtt ggtcgaggcc
     1501 gaggccggct gcgacattat cgcgccttcc gacatgatgg acgggcgcgt cgcggcgatc
     1561 cgcgcgggcc tcgatcgcgc cggcttcgcc gacgtctcga tcatggctta tgcggccaaa
     1621 tatgcctcgg ccttctacgg cccgttccgc gacgcggtcg gctcggcggc gacgctggtc
     1681 ggcgacaagc gcacctacca gatggaccca gccaataccg acgaggcgct gcgcgaagtc
     1741 gagctcgata tcgcggaggg cgccgacatg gtcatggtca agcccggcat gccctatctc
     1801 gacattctgc gccgggtgaa ggacgccttc ggcgtgccga ccttcgccta tcaggtgtcg
     1861 ggcgaatatt cgatgatcat ggcggcagcg cagaacgggt ggctcgacgg cgagaaggcg
     1921 atgatggaga gcctgatcgc cttcaagtgc gcgggcgcct gcggcgtgct cagctacttc
     1981 gcgccgcgcg ttgcggaggc gttaaaaagc gcaagctagc gccttcctca ggcgtttgag
     2041 ccgccgtgca ggcattggct cggcatcgtc tgcgcgcagc gataaccgcc aacctcaggt
     2101 gtcatgtgga cgacgcgccg cggcggctgg ccgcggcgcc gcgcgttcgg tatcgtggct
     2161 cgactggggt ctgcgaccac ggtgcgccgt gtcgccgaat gggaggaaat gaacatgtcg
     2221 aatgaatcta ttctcgtcat cttgttggtc ggcctgatcg cgggatggct tgccggacag
     2281 atcgtcaaag gcaccggcta cggcctcgtc gcggacatct gcatcggaat catcggcgcc
     2341 ctcatcggga gttggttgtt gccccagttg ggcaagcaac cgctccacgg cgctctttag
     2401 cgctctacag aacccttgta aaatggggct ttctctctat actcatccat gacgctccac
     2461 agcaatctat ccctgtttca ggacacttcc gggacacgct gcccatgaca acccgctgca
     2521 agcttgacag caagacggcc cggctcaagt tgccggtgcg caagaagccc tacaccaatc
     2581 gcgccgctcc cggcatccgc atcgcctacc gtcgcaacga aggccccggc acctggagcg
     2641 tgctcggcgg cggcggactg tggcttaaga aaatcggcat cgccgacgat ctcgagccgg
     2701 ccgacggcgt caacgtgctc gactactggc aggcgagcga ccgcgcccgc gatcttgctc
     2761 gcgccaggga tgctaacagc ggcaagtcgg tcaccgtcgt tgaggcgctc gcgacttacc
     2821 gcgacgattt gcagtcgcgt gggggcgatc cctacaacgc cgaccgagtc cttgcgcacc
     2881 tgcccaaggc gctgctgagc aaggcggttg gcttgctccg cgctaccgag ctgcggcggt
     2941 ggcgcgatgc gttgttggcg caggggctcg cgcgcggcac cgtcggacgc acgattcgga
     3001 tggccaaggc ggcgttcacc gcggctgccg acgccgatac acgcatcgcc aaccgcaatg
     3061 cctggcaggt cggactcaag gggctgcccg acgccgagaa cgcccgcaat gtcatccttc
     3121 ctgacgctat tgtgcgcaag atcaccgtcg cggcgcggcc agagggacca gaatttgcgg
     3181 tcttggttga gctcgccgcc gttacgggtg cgcgcgtcag tcagctccgg cgattgcagg
     3241 ttgccgacgt tcaagccaac cggtccgatc ctcgtgtcat gctgccgagc tcagccaagg
     3301 gccggcgcaa ggtgagtacc cgccggccgg tgccgatccc cgccgcgcta gccttacggc
     3361 tgcggcagtt gggagcggga cgttcggcgc atgctccatt gctgctcaag gccgatggta
     3421 acgcctgggg ccgcagcgac catctccgac cattccaacg cacggtcgcg cgcgctgggc
     3481 tcgatcctgg cgtgacgatc tatgcgctga ggcactccag tattgtgcgc gctctgctcg
     3541 gcgccgtgcc agcaagggtg tgcgcggcca accacgacac gtccgtggtg cagttggagc
     3601 ggacctattc caagtatatc gcggatcact ccgatgcggt ggcgcgtcat gcgctgctgg
     3661 atctggcgtc gccggccggg gctgaggttg tcccgttgtc ggggcggcgg gcgtgagtaa
     3721 gaaggcggcg aagcgcacga aagcggccac ggcgttgccg ctcgtcagcc cgcattggtg
     3781 gtcgcgcgag cagacagtgg cttattgtcg tgcgcacaag ggagagatcg ccgatggtgc
     3841 gctaaccgcg gcggtcaatg cgggggatct gccggtcaag cttgaatgga ttgaccacca
     3901 aaccagctct ccggtgcagc acaaactatt gctgtcggcc gaggactacg cgttcgggcc
     3961 gtacatcagc agcaactttt tggtaattca gccgcgccgt cccgacgttc ctccgctccc
     4021 acggccgcac gccttgtttt tctgggggcc gaaggcgaaa gagctttggc cggcgcaaga
     4081 ggcagagccg gtgacgacca agcaacaagc ggctgatccg tttaagccac cgcggcgcag
     4141 gcccggaccg ccgccggaga acgaatggaa gcaagctgtc gtgcgtgaac tgctcaggag
     4201 agccaaggcg ggcaagccac tgccctcggc gcctgatatg caggaattct gtcaggacgt
     4261 tttggagtgg gagccggatc tgcgatcaat gcaagagctt ttgcgcttgt ttgccggcga
     4321 aatgtggcac cttcttgggc ttggttagct aacgactcgc atctcctcgc atcctcccgc
     4381 ggagaaactt cgacctaacg ctatggtgcc cactgttttg gagtggagcg ccaaagatgg
     4441 ttgatatttc tgaaaatcga ccggttgatc cggccgtgga agccgcggcc cgcggtcgtg
     4501 tcgttagcaa gcgcgatcgg cgagtcaaag tcaagcagaa aacccacgac cgaccgcagg
     4561 cgcgcacacc gccggcaccg ctcggcgccg caatctgcgc tttttccatc gcggacttct
     4621 gtcgcgcaca cggtggcatg agccaggcgt tcttccacgc tctcgtcgcc aaaggcaaag
     4681 gccccaggct gatgaaggtg ggggcgcgga ctctgatcag cgtagaggca gcggccaagt
     4741 ggcgccgagc gcaggaagcc gccaccgatc aagtgaattg aagaacctca cggcccgccg
     4801 ctccgccgct cggcggtagg ccggaactgt agagcggagt gaaaagggcc ggtgacccct
     4861 caagtggttt cgaagcccga gatcaccggc ctgcaaaaca gcgcgaaggg agcgcttttc
     4921 acaatgtcac acatacaaat tcgcaaccag aagcgcaagc cgcggcggct cctctatcgg
     4981 gacggcatta ctcgcgacga gctgatgcgg ttggtcttgg aggtaggccg cgatcaggtg
     5041 gagggggttt tgaaagccct cgatctcccg accgaacctg cgcttacccc agcggagtga
     5101 acaaccagcg ccgctccgcg cgaatgcggg gcggcgacgg cctcgcgccg tgaggcggcg
     5161 cctgacgaga tcgatggttc ctgccatgaa caacattctc gcagatcgca ttctggttga
     5221 tttgatccag cagctcgacg ccgtccgcga gctccacgag caggcaaaca ccgccgatgc
     5281 gctcctgcac gcgcagactc tcgtcgacga cgcgctgcgt caggcgacca attttctgtt
     5341 tgagctgcgc cgtaagcagc taggcattta gccattcgag gaggactact cgtgcgcgtg
     5401 atcggcatcg atcccggcat tcatggcggt cttgccctcg tcaacatctg cgacggcggc
     5461 gcacccgtgc ttgtggacgc gatcgatatt ccagtgatcg gtgttaaagc gaaagagcgg
     5521 gtggacgttc tcgcgatccg tgcctgggtt caacagcatc ggccgcaaca cgcttttatc
     5581 gagcgcgcgc aggcgatgcc ccggcaaggt gcatcgtcgg ggttcaaata tggaagagcg
     5641 acgggtgcca tagaggcggc gatcgcctgt tgcgaggtcc cgatcacggt cgtcgagccg
     5701 gcggtctgga aaaagaccca ccatctccgc ggcggcgaca aggaaggtgc gcgtcagcgc
     5761 gcgctccaac tgtttccatc cgcacacgct ctgctcgctc gcaagcgcga tcataacaaa
     5821 agtgaggcag cgttagttgc gctgtacggg gcgtcgctat gaagcccttc atcgacatca
     5881 ctggagaacg ttttggccgc tgggagcgta cgatgagcgg cgcccatcgg cagaacgatg
     5941 aatatggagg acaagtcatg aataaagaag ttgagcggat ccgagttgag cgcgagctgc
     6001 ggtcgatggt cgaagagcgt ttgttcggtg atggcccgtg gtcccccgat gaccccaagg
     6061 tgaaccgggc aatatccgac aagctctccg cattggggtt gcagcatgta gagccagtca
     6121 cgggagccgt tcacacgacc gaactcggtc gggagctcaa cgtcgacacg ctgacgctgt
     6181 tcacgggcct tcatgagccg tacgaggtgc cagactgctt ggtgcctttg attacgcagg
     6241 cggaagccaa cgaattgcgc gaacgcttcg acaacggcga gcggcccgag gaggtgctgc
     6301 cgccgctgct gcgccaactg tggcgtaagc attaccaagc caagcactga caactggagt
     6361 gagtgaaatg gagatcccga ccaagtacgc cgacaagacc gtgctcaagg cctgcgctgc
     6421 gctcatgatg tcggtcgaca acgacgaaga ttttgacacc ggcgatctga tccgccgact
     6481 tgccgaaacg cttgcgccat gggctgcccg ggtcgcctgc gacgatcatc tcgccctcgc
     6541 ccggctggtc gccgaagcta tgaccctgca gttctattgc gatcgcggcg aactcttgcc
     6601 cgagtaagat gccgggaggt gcatcatgaa cgcgccgttc gggatggacc gccaagattt
     6661 cctgacgcaa gcgcatttct cgggaaatcg ccggctcctc ccgcatcaga tcacgcaagc
     6721 tatcttcgcc gtcgagcgtg cacgctcgct caacgcctcc gaaacaggaa ccggaaagtt
     6781 cctggtcgcg ctggcgatgc gccgcctgat cgagcgcgaa gccggccgca atgtgaaatg
     6841 tctctatacg tgcccgaagt ccgcacttgg acaatttgag caagaattca ttgatcacgg
     6901 ctatcggaca tttgtgttgc ggcatgggaa cgacgttgtc cccgtcgacg tcgacacggt
     6961 gctggttgca aactccacca tggtcgtaac gcatcgcgat cagctgcgca gctggcgccc
     7021 attattggtg gtgctcgacg aggccgttgc cttcaagact gcgactgcgg cgcgcaccaa
     7081 ggccgtctac ggcgacgccc tcgacggcgc cggtggaatt attgacgagg tgccgttcgt
     7141 cttggcgatg tccggcacgc tggcaccggc gcacaacggc gagctctatc cacatctgcg
     7201 tgctctcgca ccggcggcgc tcctcgacga acgtggtcga atcatgcgcc gacatgtttt
     7261 cgagaaaacc ttctgtgtgt tcggcgcgcg ccgcatcgcc ggcaaccgcg aggtacaagt
     7321 catcgtcggg tcgcgcaact caaacctgct gcgcaaacga attgacccgt atgtcgcgca
     7381 cgtcaccttg cgcgagatcg ccccgacatt gccgccggaa cgccacgagc gggtgccgat
     7441 cgcccgcgag gacgtgaagc tcgatgagtt ggcagcgatc acggatatcg aggatgcaga
     7501 gattcggagt gctgtcgaaa tgctggcaac agccatccgc gatggtctcg taccggcgcc
     7561 cgacatcgat cgtgaaatgg cgcgcttgat gcaaatgatc ggcggcggca cggcgctcgc
     7621 acatctccgg cgtgcctatg ggctggccaa gctgccctat gtggaggatg tggtgatgtc
     7681 acgtcgcggc ggcagccgcg aggagcgaac accgacgctc atcttcaaca cctaccgtct
     7741 gaccggcgat cgcctggaaa cgttactcag cgagcaggac gtcatggttg ggcgcatcca
     7801 tggcaacacc tccgccgccg aacggcacgc cgtggtttcg gggattcaag acggcagagt
     7861 cgaagcggcg attttgcaga tcgacgccgc cggctcggcg ctcaacctgc agaccgcgaa
     7921 ccggatcatc atgctcgagc caagttggac accgggagcc aaccaccagg cagtcgcccg
     7981 cgccgtgcgc atcggccaat gcaacccggt cttgatctcg tggccgacag ttcgccattc
     8041 catcgacgaa gcggtgatac gggtcctgcg ccgcaagcag gacggcctca ccgagctgtg
     8101 gcgggcggca tcatgatccg actggacgcc ctccgggaga tgatcatcga ggcgcgccac
     8161 aacgaggcga ccgtccggcc attcccgtcc ggcgtcgctg caggtgataa ccgcaccggc
     8221 ttcctcaatg cgtccgaggc aggccgatgc acacgctggc tttggtacga caaacacgac
     8281 gtcgccggcg aggctcgtca cccctacggc gtcttcgacc gcggccacgc cttcgagcat
     8341 tggtgcacca cctatctttc cgccggcctc gcgcgtgctg aaggacgcct cctctacgcc
     8401 ggcgccaagc aaaggcggct gatgctgccg gactttcgtc tcgccggcac gcccgacggg
     8461 ctgatttggt ggcgcgacca gtcagagaca gtgcttgaag tgaagtcgca cggcaccgcg
     8521 cagaactacg acagctgccc gtccgagatg catgtccgtc aggccgagct gaacatcgag
     8581 ctgtttcacg aggtcaccgc gcatcgaccc gaggatgcaa tcatcatcta cgggctggcc
     8641 gaggattatg agcgtctcat cctgcaccgc gtcgagcgac ggccggcaat cttcatcgag
     8701 atgctcggca aggcggcagc cgcctttgac gccgacaacg ccacggcagt gaccgccgag
     8761 ggcgtcgtca ccaagcagtg cgtcatttgc ccctatcgca gccgctgcgc tgccgcccaa
     8821 gcgcacgcca ttgcggcggc cgtcaccggc ggtctcgacc aggcaacgct gcgctcaatc
     8881 gatcgcctca tcgccgctcg agcgcgcgcc gtcgaggccg aggaggaggc agcgcacgcc
     8941 cgcgccgagg tggaagccga gatcatcgca acgctgaccg atggcggcgc cagcaaggtc
     9001 aagcgcaacg gctacgccgt gcagctgaag acaccaacgg acggcctcgc gccgttcctc
     9061 gaaatccgct ccgcctgaac tggcgaagcg cgctcgggcc ccgggtccga agcccctccg
     9121 agggctcccc cagacgacat cgtcaactcg agcgcataag gagcgcaacc atgcagttac
     9181 ctgctttgaa gaatgccgtc cccgcctcgc tcatcgacgg ccagtcgatc gaggacttca
     9241 tccgcaactc cacccagaac ctgcagcgca tgtccgccgg catgcccggc agcggcatcg
     9301 ccggcgccgc gtatctcggc ttcaacggca ccagcggggc gtggaaactc gaccgagagg
     9361 aggtcgaccc gaaaagcctc ggccgcatcc tggtgccgca gcatggcctg ttcgagggct
     9421 gcatcgagtg ggccaattcg tcaccgctgc agaagataca gcgacagctg ctcggcattc
     9481 actatgacga gccgatgtcc gagcggttac tgccgaaacc gctgtcgccc aacgcctaca
     9541 aaaaggaaac cgacggcccc tccttaacgc tcggcttcct cggcttcatg ctcgacgacg
     9601 gcgccaatgt cgtgtttgag cattcctcga tgggtgccaa gaaagcagtc agcgccctgg
     9661 caacgactgc cactcaggcg ctcatcgcct tcggcgagat cgtccacccg gtgatcgagc
     9721 tcggctccgg ctcatacgaa aactcctacc ggacgatcta cgaccccaag ctcaacgtga
     9781 tcggctacgt caccgacaag cgggcacggg aggtcaacgt cctctctgag gacgacatca
     9841 tcactaggcc gacggcatcg cgggctaggc tgcgccgcga gacgaaagaa gctccggcgc
     9901 tttgacacga cacggggcgc gcccgcccac gcgggcgcgt cgttttttac tcggggtggc
     9961 ccatggatgt cagagacatc acggcagaaa acgtcgctaa ggcggttggc ggcacggttg
    10021 aacgcaacgg cagcattcta tgccgctgcc cggtccacga ggcgagcggc atacacaatc
    10081 catcgttggt gctgtcgatc accgaagcac agcgcatcat atttcactgc cggtcgcaaa
    10141 actgcgacgc caagcatttt caggccatcc gcgaccatct ggttgaaaaa tgcggtttgc
    10201 cacgatcgca tgttggcggc aatagtcggg cgcagggaga gtttcactac cattatgtaa
    10261 acctcgacgg aagctactcc tggacgaaga ttaaacgcgt tacgcggacg gggaagaaga
    10321 ctttcgtctc cgcggtatat gacaagggca ccgaccagtg gacattcagc agaccaaaag
    10381 gggtgcctct gctcttcaac ctggctgcgg tcgggcaggt cctcgccgcg tacccgacaa
    10441 cgcctctgct gcttctcgag ggcgaaaagg atgtgcagac agccgggaat cttggcgtac
    10501 tcgcaacctc ctgcgccgac ggtgccggca gctggcgcat cgaggacact cgcaagctga
    10561 tcgagctggg ggtcaagaag gtcatcgtct gtcctgacaa cgacggcccc ggcatcaacc
    10621 acggcatcga gatcgccaaa tcgttccagc aagcaaagat cgaagtctgc tggctgagtt
    10681 tgcctgctaa ggacctctcc gagtgggagc caaagcaagc gtaccctgac gcgctgctca
    10741 aagagctgat ggacgcggca ccgccgttcg atgctgacgc gctcgactgg tgcagccgcc
    10801 tgaagagggc gcggccgaac gccggctgca gctatcgcgg cgacctcccc aatgccttgc
    10861 tagcgctcca attcgagccg cgcctcaagg ggtgctttgc ctggaatgac tttcgacacc
    10921 gggtcgaggt agtgcgcaag acgccctggt gcttgccgga ctggtgggag gcggcggcgc
    10981 tgacgcccgt cggccaccgg gcgctgtgcg acgccgacat ggccgagctc agtagctacc
    11041 ttacgctcac ctatgatttt ggcgcctgcg ccgtgtcgac gtgccggcac gccctccaca
    11101 ccgaggccaa gcggcatagc ttcgatgaac ttaaagaccg gcttgatggg ctaccgggct
    11161 gggatggcag gcaccgcctc aacggctggc tggtgaccta cgccggcgcc gatgcgcaag
    11221 cgcacacgaa ggaatatcta gagctgattg gctcgaaatt catcatgcag gtgctgcacc
    11281 gggcactcaa ccccggcgcc aaggcggact actcagtggt cttcgtcagc atccaaggaa
    11341 ctggcaagga cctggtgttt gatgcgatgt tctctcctta ctaccgcgag ggcattccct
    11401 cgccgcgcgt gagccaggct gacttcgccc ttgccctcgc cggtggcatt gttgcccacg
    11461 cagccgaaat gtcggcctgg cgcaaggccg acgtcgaaga ccagaaggcg gcgttgacgc
    11521 gttgtgtcga ccatggtcga cgcgcctacg gctacgaggc gtgtacctac ccacgacgta
    11581 cctgtctcgc cttctccacc aatgacgtcg aatgcctggc cgacaccact ggcgaccgcc
    11641 gctactggaa tgtctgcata gagcgcggcc ggatcgacat cgaggcgctg cgccgcgatc
    11701 gcgaccagct cctggccgag gcactgcacc ggctgaagga gggtgagcag cattggccga
    11761 caccggagga ggaggagcgc tggattgtcc ccgaacggca gaaaaacatg cccgcggcgg
    11821 cgctggagat cctcgctatc ctggagcggt tcatcgtcga ggagccgcag acgacgcgac
    11881 cgaaccgcgg cgacttcgcc tggaaatggg agccaagacc acaacctttg agcgagctgt
    11941 atgtcgacgc cttcttcgaa aagtgttttg gaatgtacgc gggggtcaga cgccaagggc
    12001 tcgaccgggc ctccaagagg gatgtcgact actgtacgac gtacctccgc gagcacgact
    12061 ggcgacgcgt acggaggctc ttgccggacg ggcaaaaggt acagatttgg tgtgctccca
    12121 ccccctccgg gggcggtgtt tttcgtggac acccaagctc cgagcttggg tgtccacaga
    12181 caaaagatgt ggaattggca tcacaggatg cgcaacaaaa ttgcgcggac gcgcctgact
    12241 tggcggtcac gacgaccgtg gacacccaac ggacacccaa gatcgctcgt tgggtgtcca
    12301 caagaaaccc gctgcgctct agcacttctt ctgttttgga cacccaacca cccaaaacgc
    12361 tattaaacga cgttttcaga tcgatttttg attcgggagg ggaaacgcaa tttacttttg
    12421 aaatagcact aggaaaaatt cttgggtgtc cacgggccct cgacgggctc ttgcccgtca
    12481 cgcttgacag tctcgaagag cacttcccgc gcgatcgttt cctctccctc gacgtggaga
    12541 cgaccgggct gtcggcgggc tgcgacggcg tgcgcaccgt gcagattgcc gacggtgaga
    12601 gtgccgccat ggtggtcttc gaccgccctg tcgcggcgcg cgcgctggtt gtactggccg
    12661 attttctccg gggtcgtcgc gtcgtcgccc acaacgcccg ctttgaggcg agctggctgc
    12721 agcaggctgg tattgacctc gttcttgacg ataccgtcct gctgttctcg gcggtgcggg
    12781 ggacccgctc tccgaagggt gacaagtata tcggcggcgg tcgtgtctcg ctcgccgcgc
    12841 tggcggtcat ggtgcttggc gagacgctcg ataagtccga gcaggtctcc gactgggcgg
    12901 cgccgacgct gtccgcgtca cagcttacct acgccctcaa tgacgccatc gtcacgcacc
    12961 gcatctggga ggcgttgcgt gccgagctgc attgcaagag caagcagcac ggcgtcgaca
    13021 tcgttgccgg ttatgaggat atgcggtttt ccgctgccat ggcgcaccgc atggagtgcg
    13081 tcggtatcgg ctttgacgtt gctgcccatc aggcgtggat tgcccgtaag cagcagccgg
    13141 tggcggtgct cgaggcgcat cttgccactc tcgatccggc actctcttcc gcctgtatcg
    13201 cttcgggggt gcagctcgat cggctgtttc ggcaacgtct cgagctctac gccgcccagg
    13261 atcggcggcc ggcgttgctg gcgtggccaa aaacggagaa agctcggcgg ttatcgtttg
    13321 gtcgtgaaga tcttgccgcg gttctcactg ccaatcgttt gcagggtgcc gagcggcaac
    13381 tggttgaggc gctctatacg cgggccgagc aggtgcgctg cctggcgacg tttggcacgg
    13441 cgttttccag tcatgtcatc gatgggcggc tgcatggtca attgcacgcc ggcgggacgg
    13501 ttaccgggcg ctataccagc tccaacccca atttgcagaa cattccgacc gacttggaat
    13561 ttcgcggttt cttctgtgct cccgctggtc gtgccttcgt tgacgttgac tattcccagc
    13621 tcgagctgcg cgtcttcgcg gcgctctccg gcgacgccaa gatgattgcg gctttcgagg
    13681 ccggttggga ttatcacgat ctggtcatgc agcgtctcgg ctgtacccgg cggcaggcca
    13741 aggctgtcaa tttcggaatc atttttggca tgggtgcagc gacgctggcg gcggatcttg
    13801 gcatcgatga ggtgaccgcc ggcgaatatc tgcgcggctg ggatgagcag gccccgatcg
    13861 gcgccgagtg gcgcgcttcg ctgccgcgtc tctacgtcgc ggaacaaggt gtgcgtacgg
    13921 cgcggcgctg gatcgattac ctcgatgatg atgatgctga tgtcgcggca aacactcgac
    13981 cgaagaatta cccggtgcag ggtggcgcgg cggatgtcat gcaccgggca atgcggctgt
    14041 tgtttgagca ctatcgcgat tggccgggta atgtgctgcc ggtgttgacc gtgcatgatg
    14101 aggttcttgt cgaggtgaac attgccgttg ccgatcgagt tggctccctg ctggctgatg
    14161 tcatggtcga ggctttccgt gacgtgctgc caaacgggcc gatgcggttc ctggctattc
    14221 ctggtgttgg gccaacttgg gcggcggcga aggccgacgg tgagatgcgg gaaaaggcgc
    14281 tgcggacggc acgttgtgcg taactgcttg tgtaattacc tacggattgg gtggtgctct
    14341 tcaagttgga ggagcgccgg aattgatcgg cgggcctaag aggggcgtct ggaatattcc
    14401 ggataatgtt ccgcctgttc cgtctgacaa aagttgcctg aaaatactcg gatattcgcc
    14461 cgggaaaata tttggggagt gagtttttgg tatttgccgc cttaaaaaat agtctgttgg
    14521 gtgagtttcc agaatattcg cggcgtttgc ggcactttat ttgttcagtg ggaggcctcg
    14581 agcgatcttc cattcgcgtt tgtcgtgtcg ccacgttgga atggggatca accacccagt
    14641 ttcgacgagg gcccttgtca ggttcaggat cctttttgtg tcgttgcgta ggggggtggg
    14701 tccgaattta ccgatagcgc gcgcggtaat ggtgggttgg tcccaatgtt tcacaatcca
    14761 ttcgaggagt tgcgtagttt ggtcttcgcg cgccggttcg gtcggtgttg gctgcggtcg
    14821 ctcccgattc agccggtcaa aaattgtcag cgccgccatt cgtcgctcgg tgattcgctg
    14881 tgatcatctt cgatttgttt cggggtactg ggccggggcg ctgcttcgat tattaaaacg
    14941 tcggctggac tgctctccat cccctttgcc cgtacatctg cgccgaggtc gattttgatg
    15001 taaattttct cttgttctgc tgcggcttct ggtccgggat cggcgagtcc attcaaaact
    15061 ttgcttgcg
//
LOCUS       DATLJT010001071         6679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3061354,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001071 DATLJT010000000
VERSION     DATLJT010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061354"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1077
                     /locus_tag="VM910_24655"
     CDS             94..1077
                     /locus_tag="VM910_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65746.1"
                     /translation="MKALAAFAGLEGLFDLTEGNGVDMCATLLRVLTDLYLQRPIHTP
                     EDEHYYTELALRLIDATEISERAALAARLASYPSAPKPVLERLARDVIEVAGPILEHS
                     PCPAELEAAAEARAGAYAETAATRPGSAAQTVRAAGPVNVAEARELSELFYAAKAPER
                     RLILINLDYAPLISSQPLPAMQRTDVWRLEATALEHNAETLIGELERVLAVSRAQARR
                     IVNDELGEPIVVAAKAMGLPAEVLQRLLLFWSSWVGQPVDRICELADLYDEIRTDAAH
                     RLIVIWREDKRAEDEPVQQEPGPRGSGDDLIEKVTPGAVLDLEDPRVGVET"
     gene            complement(997..1344)
                     /locus_tag="VM910_24660"
     CDS             complement(997..1344)
                     /locus_tag="VM910_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HVI65747.1"
                     /translation="MRLKSAVWVAAYIRRCHIEGAFAAVRRRGAEEAGAIFVKLNRLD
                     GTAELFGPAPQSVFDDARPAERVFSRCLGEAPAPEAKIEERLARELRFDPDAWILEVE
                     DRAGRHFLDQIVA"
     gene            complement(1458..2246)
                     /locus_tag="VM910_24665"
     CDS             complement(1458..2246)
                     /locus_tag="VM910_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HVI65748.1"
                     /translation="MTRVSREKSVTLDGGRVSRLLRALGWRVWDAVGFVLGAFVAIAI
                     LVNVLFMQPGSHPAPMFKAMLAQGRAGVVTGSTSAAIPRPRPIESAALPAATKFAPPS
                     TPRAPGEIINDIQRELARRGYYDGVIDGLYGPKTDAAIRDFEQAAALKPSTEPNEALL
                     QAILRAPAKQAKASTAAPSGARPMPAPSDSGVERAAASKRVIALQRALAEYGYGQIKA
                     SGTVDAETQAAIEKFERERKLPITGQPSDRLVRDLAAITGRPLE"
     gene            2778..3176
                     /locus_tag="VM910_24670"
     CDS             2778..3176
                     /locus_tag="VM910_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HVI65749.1"
                     /translation="MRFLLRMAFWLTVILALLPSAGSQPTPKVNVSAIEAVSAAKATV
                     TDVGSFCERQADACNVGSQAAVVIGNRAQAGAKMLYEFLNEHLGSHDAETANGASAVP
                     LPVARPSQHTLWPSDLVPAWRSAPSRKEGT"
     gene            3340..3798
                     /locus_tag="VM910_24675"
     CDS             3340..3798
                     /locus_tag="VM910_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HVI65750.1"
                     /translation="MTAASSETFPATLEEIVDNFSLLEEWDDRYRYVIELGRGLRPLA
                     ERDRTQANKVQGCASQVWLATNVHPNGQGGPILTFTGDSDAHIVRGLIAILFAMFSGK
                     QAGEILSTDAIAVFERLGLREHLTPQRSNGFRSMVERIRADARSALARTA"
     gene            complement(4049..4465)
                     /locus_tag="VM910_24680"
     CDS             complement(4049..4465)
                     /locus_tag="VM910_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HVI65751.1"
                     /translation="MSNNTSGGTFIELTASIVSAYVSNNSVPASDLPALIDQVHAALS
                     RVLRGHGDVRGEPPKPAVSVKKSITPEYIVCLEDGKKFKSLKRHLRTQYNMTPEQYRE
                     RWGLAPDYPMVAPNYAAARSRLAKQMGLGQQRRRRR"
     gene            complement(4762..5700)
                     /locus_tag="VM910_24685"
     CDS             complement(4762..5700)
                     /locus_tag="VM910_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65752.1"
                     /translation="MSVILSLLGIAVAAAGVAAIGFGIPINEFTLGTTLIVAGVGALT
                     GGLVLIGLGAVVAELGRLSETIRTRLVVRPQVRAEAPELAQPAPAPANLAGAPVPMVS
                     TRPAQPAPARARSEAAQAPQALDISAAAAERSQPSRAEQAEPSIVADEEEVPLSPLGV
                     AHLQTRRPRPVEGASPEPKAASEEGGSAVEALKASRLDFLFRSKPARSASQRENFDAV
                     WPADTRPARRAENEASPPVEADQRQAEAGLGAEDRAQQSVTLDEPRAAAILKSGVVDG
                     MAYTLYTDGSIEAKLPHGTVKFGSIAELRAYIESNS"
     gene            6038..6640
                     /locus_tag="VM910_24690"
     CDS             6038..6640
                     /locus_tag="VM910_24690"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HVI65753.1"
                     /translation="MSFTLPDLPYQYDALAPYMSRETLEYHHDKHHLAYVNNANNLIK
                     GTEWEGKSLEDVVKGSFGKNAGLFNNAGQHYNHLHFWQWMKPNGGGDKLPGNLEKKIS
                     ADLGSVAKMKEDFIQAGVTQFGSGWAWLAVKDGKIIVTKTANGENPLVHGGKPILGCD
                     VWEHSYYIDYRNRRPDYLKAFVDHLVNWEHVAKMYDAAMK"
BASE COUNT         1257 a         2131 c         2055 g         1236 t
ORIGIN      
        1 cgatttgaga cgcgcaaggt taacggtctg ctaacgccgg gcccctaagc tatttcaaat
       61 tcaaagtgtt gcagcaaagg cgcagtgacc ataatgaagg ctctcgcggc attcgctggg
      121 ctcgagggcc tgttcgatct cactgaggga aatggggtcg acatgtgcgc gaccctgttg
      181 cgagtactca ccgatctcta cctgcagcgg ccgatccaca cgccggaaga cgaacactat
      241 tacaccgaac tggcgttgcg gctcatcgat gcaaccgaaa tttcggaacg ggcggcgctt
      301 gccgcgcggc ttgcatccta cccatcggct cccaagcccg tgctcgagcg tttggcgcgc
      361 gatgtcatcg aagtcgcggg tccgatcctc gagcattcgc cgtgcccagc cgagctcgag
      421 gcagccgccg aggctcgcgc cggcgcttat gccgagactg ccgcgacacg acccggcagc
      481 gctgcgcaaa ccgtgcgagc cgctggccct gtcaatgtcg ccgaggctcg cgagctgtcg
      541 gagctgttct acgcggcaaa agcgcccgag cgccgactga tcctgatcaa tctcgactac
      601 gcaccgctca tctcatcgca gcctctcccc gccatgcaac gcaccgatgt ctggcgcctg
      661 gaagcgaccg cgctggagca caatgccgag acgctcatag gagagctcga acgcgtgctc
      721 gcggtctcgc gggctcaagc gcgccggatc gtcaacgacg agctgggcga gccgatcgtg
      781 gttgccgcca aggccatggg gctgccggcc gaggtattgc agcgcctgct gctgttctgg
      841 agttcctggg tcgggcagcc ggtcgatcgc atctgtgaac tcgccgatct ctacgacgag
      901 atcagaacgg acgcggcgca tcggctgatt gtcatctggc gcgaggacaa gcgggccgag
      961 gacgagccgg ttcagcagga gccaggcccg cgcggttcag gcgacgattt gatcgagaaa
     1021 gtgacgcccg gcgcggtcct cgacctcgag gatccacgcg tcggggtcga aacgtagttc
     1081 gcgcgccaga cgttcctcga tcttggcctc cggggcgggc gcttcgccaa ggcaccggct
     1141 gaacacgcgc tctgctggcc gcgcgtcgtc gaagacgctc tgcggcgccg gcccgaacag
     1201 ctccgcggtg ccgtcgagcc gattgagctt gacgaaaata gccccggcct cctccgcgcc
     1261 acgccgccgc acggccgcga aggctccttc gatgtggcag cggcggatat aggcggcgac
     1321 ccagacggca gatttcaggc gcattacaaa ttctcgtcat gcccgccgcg accgagccga
     1381 atagaccaca tgtgcgaggg ggacgagagc cccgatcgtc attttttcga ttacgaaagc
     1441 gctcgcgcga cgaaatctta ttcgagcggt cggccggtga tcgccgccag atcgcgcacg
     1501 agccggtcgg agggctggcc cgtgatcggc agcttgcgct cgcgctcgaa tttctcgatc
     1561 gctgcttggg tttccgcatc gacggtcccg ctcgccttga tctgaccgta gccgtactcc
     1621 gccagcgctc gctgcagcgc gatcacgcgc ttcgatgccg ccgctcgttc gacaccacta
     1681 tcgctcggcg ccggcatcgg acgcgcgccc gatggggccg cagtgcttgc cttcgcctgt
     1741 ttggccggag cccgcaggat tgcctgcaac agcgcctcgt tcggctccgt gctcggcttg
     1801 agggcggcag cctgctcgaa atctcggatt gcggcgtccg ttttcgggcc atagaggcca
     1861 tcgatgacgc catcgtagta gccgcggcgt gccaattcgc gctggatatc gttgatgatc
     1921 tcgcccggcg cacgcggcgt ggacggcggg gcgaacttcg tcgctgcagg aagagctgca
     1981 gactcgattg gccgtgggcg cggaatggca gccgacgtgc tccccgtcac cactcccgcc
     2041 ctgccctgcg cgagcatggc tttgaacatg ggcgccgggt gcgagccggg ctgcatgaac
     2101 agcacgttga ccaggatggc tatcgcgacg aaggcgccga ggacgaaacc tacggcatcc
     2161 cacacccgcc aacccagcgc tcgcaatagc cggcttacac ggccgccatc gagcgtgacg
     2221 cttttttctc gactgacgcg tgtcatcgtc tacgcaccgt gttgcgcgat accggcacgc
     2281 cctcgcgatc ctgcacgacg ctatcgcagc ctgccgcagc gctgggcatg gcaatccccc
     2341 caaaatgatc tcggatcaaa ttcacgcttt cgcgcgcttc ggacgatgtt ggacacgctc
     2401 cctttgccag gatcgtgtca gaggccgctt aagcgaacgc taacagcgac tctcagcggg
     2461 gtcggccttc tttcgttagg attcgcgacc gcattcgcat gtgatggtga acggtaagtt
     2521 tccggcccct accacgcgct ggtgctcgag ccgatgccgc ggttgcacgc aacaacgcgc
     2581 tttaccgatg tttcagtgcg cagcggcggc tcaaatcaat tttagtcaaa tccggatggt
     2641 tcgaaatttg tgaaaaacca ctgttgattc gcgccgaata ccgacaaaaa gcattgggcg
     2701 ctacaaacca gcattttaac tgtcgctgat agcatcgacc gcaataggcc gaacatcagc
     2761 ccagacgaaa gcgaagcatg cgatttctct tgcgaatggc attttggctc accgtcattc
     2821 tggcattgct gccgagcgcc ggatcgcagc cgacgccgaa agtcaacgtg agcgccatcg
     2881 aggccgtgtc ggcggcgaag gccaccgtca ctgacgtggg gtcattctgc gagcggcagg
     2941 cggacgcttg caacgtcgga tcgcaggcag cggttgtgat cggcaatcgc gcgcaagctg
     3001 gagccaagat gctctatgaa ttcctcaacg agcaccttgg ctcgcatgat gcggagacgg
     3061 cgaacggcgc gtccgccgtg ccgttgcctg tcgcacggcc ctcgcaacac acgctgtggc
     3121 catccgattt ggtccccgcg tggcgttccg caccgtcgcg caaggaaggt acgtaaggct
     3181 ctctcggttc agtccccatt cagaactgga gacggctgcg tgacgggccg aattcggccc
     3241 accgggaacc gattggcgat gcgccgccgg atcgattttt cgccagccgt cattcgcccg
     3301 cactaccatc atccctatat attcgctatg ccccaagcca tgactgcagc ctcatccgag
     3361 acgttcccag cgacgctgga ggaaatcgtc gacaactttt ccttgctcga ggagtgggac
     3421 gaccgctacc gttacgtcat cgagcttggc cgcggcctgc gtccccttgc cgagcgcgac
     3481 cgtacacagg cgaacaaagt gcagggctgc gcgagccaag tgtggctcgc taccaacgtg
     3541 catccaaacg gtcaaggcgg acccatactc accttcaccg gcgacagcga tgcccatatc
     3601 gtgcgggggc tcatcgccat cttgttcgca atgttttcgg ggaagcaggc gggcgagatt
     3661 ctttcgaccg atgcgatcgc ggtattcgag cggctgggct tgcgtgagca cctcacccca
     3721 cagcgatcga acggattccg ctccatggtc gagcgcatcc gcgcggacgc ccgctcggca
     3781 ctggcaagga cggcgtaagg cgcctccgcg aaatattgcg ccggatcggg tgggcgccgt
     3841 ggaaccggct cggcacttgc ctgtctacct tgcggtggcc tcgatcgaac cccccaagga
     3901 acgaaagagg ctgctaggcc ccggcgacaa cctccccggg gccttccatt ttgccggtaa
     3961 ggcatgcgag caaacgctca gagatcacgc cgatggcacg tcggcggcag tttgatcgca
     4021 tcaccgcgcc gcgatcccgc ttcatcagct atctgcggcg ccgccgctgc tggcccagcc
     4081 ccatttgctt ggcaagacgt gaacgggcgg ctgcatagtt cggcgccacc atcggatagt
     4141 ccggagccaa cccccatcgc tcgcgatatt gctccggtgt catgttgtat tgcgtacgca
     4201 ggtgacgctt gagggacttg aattttttcc cgtcctcgag gcagacgata tattccggcg
     4261 tgatcgattt cttgaccgag accgcgggtt tcggtggctc gccgcggaca tcgccatgcc
     4321 cgcgcaatac ccggcttaag gcggcgtgca cttgatcgat caatgccggg agatcggaag
     4381 ccgggaccga attgttgctg acataggcgg agacgatgga ggctgtcagc tcgatgaaag
     4441 tcccgcctga ggtattgttg ctcattgagt atcgtttcct gccgcgatct tcggcttgcg
     4501 cagcagaagc cgcaatttct gcgtattcaa tgtcgttcta ttcttgcgcc ctgagaaagg
     4561 caagaagtac tgccgctgtc gagcaaaata atgggagcgt gattattggc gatcttctga
     4621 ccgataacag aacaaacttg ttcgagaact gacgtcacgc tcgacgtctg ccgaccggca
     4681 acaggcggcg gcacgcggcg atcctgcaaa atgcacaggc ccgcaggaga aaccgcttgc
     4741 gttcgagggg cggccggagg gctacgagtt gctttcgata taggcgcgca gctcggcgat
     4801 cgagccgaac ttgaccgttc cgtgcggcaa tttggcctcg atcgatccgt ccgtatagag
     4861 cgtgtaggcc ataccgtcga caaccccgga cttaaggatt gcggctgcgc gcggctcgtc
     4921 cagcgtgacg gactgttgcg cgcgatcctc ggcgcctagg ccggcttctg cctggcgttg
     4981 atccgcctcg accggcggcg acgcttcatt ttccgcacgt ctcgcgggtc gggtgtccgc
     5041 gggccacacc gcatcgaaat tctcgcgctg ggacgcggaa cgcgccggct tcgaacggaa
     5101 caggaagtcg aggcgcgatg ccttgagcgc ctcgacggcc gaaccgccct cttccgacgc
     5161 agccttcggt tcgggtgacg cgccctccac ggggcggggt cgacgcgtct gaagatgagc
     5221 gacgccaagg ggagagagcg gaacctcttc ctcgtcagca acgatcgaag gctcggcttg
     5281 ctccgctcgc gaaggctgcg accgctccgc tgccgccgcc gaaatgtcga gcgcctgagg
     5341 ggcctgtgcc gcctccgacc tcgcgcgggc gggcgcgggc tgagctggac gggtcgagac
     5401 catgggcacc ggagcaccgg cgaggttggc cggggcaggg gctggctgcg cgagttcggg
     5461 cgcctcggca cggacctgcg gtcgaacgac aagccttgtg cggatcgtct cgctcaagcg
     5521 accaagctcg gcgaccacag ccccaagccc gatcaagacc aacccgccgg tgagcgcgcc
     5581 gacgcccgcg acgatcagcg tggttcccag cgtgaactca ttgatcggaa tgccaaatcc
     5641 aatcgcggcg acacccgctg cggcaaccgc aatgcccaat agcgataaga tcacagacat
     5701 gttttacgcc ccgcccggcc cgcgccatct tcggcagcag ccgtggttgc tatggttacg
     5761 aaagaatttt gccgccgtta cgcgtcaacc gcaataacta atgtggcgcc ccggcgcgca
     5821 aatccggtct tgattaacat tgggcaggag gcgaactcgc gccgaaggta gcgtatcggt
     5881 gcatagccaa gccgccgggc gtctggccgg cgggtccgtt gccccggcgc ccgctccggc
     5941 ctaatgagtt agcgcggaac cgaggctggc gtgcacccgt tacccataga acccccctcg
     6001 cctcgtccga ccgtttgatc atttgaggag taagccgatg tctttcacgt tgcccgatct
     6061 tccctaccag tatgacgcgc ttgcgcccta catgtcgcgc gaaacgctgg aatatcacca
     6121 cgacaagcat cacctcgcct acgtcaacaa cgccaacaac ctcatcaaag gcaccgagtg
     6181 ggagggaaaa tccctcgaag acgtcgtgaa gggctcattc ggaaagaacg ccggcttgtt
     6241 caataatgct ggccagcatt acaaccactt gcatttttgg cagtggatga agccgaacgg
     6301 cggcggcgac aagctgcctg gcaaccttga gaaaaagatc agcgcggatc tcggctcggt
     6361 cgcgaagatg aaggaggact ttatccaggc gggcgtgacg cagttcggct cgggttgggc
     6421 ttggttggcc gtcaaggacg gcaagatcat cgtcaccaaa acggcaaacg gcgagaatcc
     6481 gctggtgcat ggcggcaagc cgatcctcgg ctgcgacgtg tgggagcact cctactacat
     6541 cgactatcgc aatcgtcggc cggactatct caaagccttc gtcgatcatc tggtgaactg
     6601 ggaacacgtc gccaagatgt acgacgcggc aatgaaataa ttgctctgct gtcatttgga
     6661 cttatcgtcg gcgcccctc
//
LOCUS       DATLJT010001072         7663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_761603,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001072 DATLJT010000000
VERSION     DATLJT010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761603"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VM910_24695"
     CDS             complement(<1..1044)
                     /locus_tag="VM910_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HVI65754.1"
                     /translation="MSSPTTSLLERAGLDRGDVRRHLARGLEGADDGELYLEYAQSEM
                     LVFDNGRLKQATYNTDQGFGLRAVKDEAVGYAHSSDLSEAALARAADAVRAVKGGYRG
                     AYAEPPGRTNVRLYGDDNPLGAPGFETKARLLEGIDAYARARDPRVRQVSISFGASWQ
                     VIEILRGDGESYRDVRPLVRVNVSVVVGKGDRQEAGSHGYGGREDFSHFLATQAWQGA
                     ADEAVRQALINLEAVPAPAGEMDVVLAPGWPGIMLHEAVGHGLEGDFNRRKTSAFAGL
                     MGQRVASKGVTVVDDGTIAQRRGSLSIDDEGTPTSRTVLIEDGILVGYMQDRHNARLM
                     GMKPTGNGRRQSYA"
     gene            complement(1158..1673)
                     /locus_tag="VM910_24700"
     CDS             complement(1158..1673)
                     /locus_tag="VM910_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI65755.1"
                     /translation="MLFRTMKSLLAATAMALVLSAGTAERATAQGAVKSVHNDWQVRC
                     DTPPGAQGEQCALIQSVTAEDRANVGLTVIVLKTADQKSRLMRVVAPVGVLLPSGLGL
                     KIDQADVGRAGFVRCLPNGCVAEVVMDDNLIKQLRSGQTATFIIFQTPEEGIGFPMSL
                     KGFGEGYDKLP"
     gene            2049..2900
                     /gene="coxB"
                     /locus_tag="VM910_24705"
     CDS             2049..2900
                     /gene="coxB"
                     /locus_tag="VM910_24705"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVI65756.1"
                     /translation="MLVLKRLTVLAITMVAILTGAESALAGLGQPSPWQVGLQQSASP
                     VMDNIIWFHDFLLYIISGIAGFVLVLLVVVMVRFNARTNPSPSRTTHNTLIEIAWTLI
                     PIVVLMVIAVPSFKLLFLQLNVPAADLTVKATGKQWYWSYSYPDNGQFEFDSLMLKEG
                     ERKEGQPRLLAVDNEMVVPVNKTVRVITTGSDVIHSFAVPSFGIKIDAVPGRINETWF
                     TATREGVYYGQCSELCGKDHAFMPIAVHVVSEQAFSAWVEEAKKKYARDEALPPTTVA
                     AAQSTPR"
     gene            2954..4558
                     /gene="ctaD"
                     /locus_tag="VM910_24710"
     CDS             2954..4558
                     /gene="ctaD"
                     /locus_tag="VM910_24710"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HVI65757.1"
                     /translation="MAAMAKAHGEDLPDHPHGWRRYLYSTNHKDIGTMYLVFALLGGL
                     VGGVLSIGMRLELMHPGLQFFHDSHMFNVFTTAHGLIMIFFMVMPAMIGGFGNWMIPL
                     MIGAPDMAFPRMNNISFWLLPASFTLLIISLFVEGEPGAPGVAAGWTIYAPLSTTGHP
                     GPAVDFAILSLHLAGASSILGAINFITTIFNMRAPGMTMHKMPLFVWSQLVTVFLILL
                     AMPVLAGAITMLLTDRNFGTTFFTPGGGGDPLLFQHLFWFFGHPEVYILIVPGFGMIS
                     QIVATFAKKPVFGYLGMAYAMVAIGGIGFVVWAHHMYTVGMSTAAQAYFVVATMVIAV
                     PTGVKIFSWIATMWGGSLEFKTPLLWAIGFIFLFTVGGVTGVVLANAGVDRSLQDTYY
                     VVAHFHYVLSLGAVFTVFAGWYYWFPKMSGYMYSETIGKLHFWLTFIGVNIVFFPQHF
                     LGLAGMPRRIADYPDAFAGWNLVSSLGSYLSALGALVFFFGVAHAFMRKERAADNPWG
                     AGATTLEWTLPSPPPFHQYNELPLIK"
     gene            4608..5558
                     /locus_tag="VM910_24715"
     CDS             4608..5558
                     /locus_tag="VM910_24715"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVI65758.1"
                     /translation="MSLVSEGAEFAGRRVASAGYGPSIASVGDYVALMKPRVMSLVVF
                     TALVGVMIAPGHIHPVIGFTALLCIAVGAGAAGALNMWYDADIDAVMRRTSNRPIPRG
                     RIAPGEALAFGIVLAAFAVGVLGLLVSWLAAALLAFTIFFYVAVYTAWLKRSTPQNIV
                     IGGAAGAFPPIIGWVAATGGISVEPCLLFLIIFFWTPPHFWALSLVRVDDYARARVPM
                     LPVVAGPEETRRKILLYSIGLVPIGAAPWLLGYAQIIYGVSALLAGALMVAFAWRVHR
                     ERDGERAERAAHDLFAFSILYLFVLFAVLLVEGGVIGHAV"
     gene            5572..5730
                     /locus_tag="VM910_24720"
     CDS             5572..5730
                     /locus_tag="VM910_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoxF protein"
                     /protein_id="HVI65759.1"
                     /translation="MDEKPKGIVLSEEQKRSRRARSIAIALALGALVVLFYVVTLVKG
                     PAVLNRPL"
     gene            5736..6335
                     /locus_tag="VM910_24725"
     CDS             5736..6335
                     /locus_tag="VM910_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVI65760.1"
                     /translation="MKVPNISSQRRRDVMVAAACGVFVAAMVGAAYAAVPLYNWFCRT
                     TGFGGTTQVATAAPSHVLDRKLKVRFDANVAGGLPWRFEPDQNSIVVKIGEVVTVSYR
                     VVNESARETVGVASYNVSPPTAGAYFSKINCFCFTDQRLKAGEKRDMTVVFFVAPELA
                     KDFEQDGLDTITLSYTMYPARQAETPQVDNGAALAPGRS"
     gene            6398..7261
                     /locus_tag="VM910_24730"
     CDS             6398..7261
                     /locus_tag="VM910_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVI65761.1"
                     /translation="MAEAHAKPHHDYHLVAPSPWPAIGAVSAFLAAVGGITWMHHMFA
                     AAPYIFGVGVIGIAYTMIGWWTDVAREATFEGYHTRIVQISHRYGMILFIASEVMFFV
                     AWFWAYFNSALFPADMHQITREQLFGGVWPPKGTETFDPWHLPLLNTLILLTSGTTVT
                     WAHHALLENDRQGLKWGLILTIVLGLAFTCVQAYEYSHAGFNFSGNIYGATFFMATGF
                     HGAHVIIGSTFLIVCLLRVYLGHFTPTQHLGFEFAAWYWHFVDVVWIFLFSCIYVWGS
                     GGASVAHAAGH"
BASE COUNT         1266 a         2373 c         2419 g         1605 t
ORIGIN      
        1 agcatagctt tgccggcgac cattgcctgt gggcttcatg cccatcaggc gcgcgttgtg
       61 gcgatcctgc atgtagccga cgaggatgcc gtcctcgatc agcacggtgc gactggtcgg
      121 ggtgccctcg tcgtcgatcg acagcgagcc gcgtcgctgc gcgatggtac cgtcgtcgac
      181 aacggtgaca cccttcgacg caacgcgctg tcccatcaga ccggcgaatg cagaggtctt
      241 cctgcggttg aagtcacctt cgagcccgtg tccgaccgcc tcgtgcagca tgatgccggg
      301 ccagccaggg gcaagcacca cgtccatctc gccggctggc gccggcaccg cttcgagatt
      361 gatcagcgcc tggcgcacgg cttcgtcagc ggcgccctgc caggcctggg ttgcgaggaa
      421 atgggaaaag tcctcgcgcc cgccatagcc gtggctgccg gcttcctggc gatcgccttt
      481 gccgacgacg accgagacat tgacgcgcac cagggggcgc acgtcgcggt agctttcgcc
      541 gtcgccgcgc aggatctcga tcacttgcca gctcgcgccg aatgagatcg aaacctgtcg
      601 cacgcgcggg tccctcgccc gggcatatgc gtcaatgccc tccagaagcc tcgccttggt
      661 ttcgaagcct ggtgcgccga gcggattgtc gtcgccatag aggcggacat tggtgcgtcc
      721 aggcggttcg gcgtaagcac cgcgatagcc gcccttgacc gcgcgcaccg cgtccgccgc
      781 gcgcgccaac gctgcctccg agaggtcgga ggaatgggca tagcccaccg cctcgtcctt
      841 gacggcgcgc agcccgaagc cctggtcggt gttgtaggtc gcctgcttga gcctgccatt
      901 gtcgaaaacc agcatctcgg actgggcata ttcgagataa agctcaccgt cgtccgcgcc
      961 ttccagacct cgggcgaggt gtcggcgcac gtccccgcgg tcgaggccgg cgcgctcgag
     1021 aagcgacgtc gtgggtgagc tcatgacggc atccgcattc gcacagtcta gccaaagata
     1081 ggcgccctcg cctggagttg cgagatcgcc acgccgctaa atcgaactct aagggaagga
     1141 cacctcgcct gggcgtctta cggcagcttg tcgtagccct cgccgaaacc cttgaggctc
     1201 atcgggaagc cgatgccttc ctcgggcgtc tggaagatga tgaacgtcgc ggtctggcca
     1261 ctgcgtagtt gcttaatcaa gttgtcgtcc atgactacct cggcgacgca cccgttgggt
     1321 aggcaccgga cgaagccggc gcgaccgacg tcggcctgat cgatcttcag tccgaggccc
     1381 gagggaagca acacgccgac gggcgcgacc acccgcatga ggcggctctt ctggtcggca
     1441 gtcttgagca cgattacggt gaggccgaca ttggcgcgat cctcagcggt gacgctctgg
     1501 atcagcgcgc actgctcgcc ctgcgcgccg ggtggcgtgt cgcagcggac ttgccagtca
     1561 ttgtggaccg acttgaccgc gccctgcgca gtggcgcgct cggctgtgcc ggcggagagc
     1621 accagcgcca tcgcggttgc tgccagcaat gatttcattg tgcgaaaaag catttccggg
     1681 ctgaatgaac ccataatctc cgcctcgaat gctgcctgcg ccccgctatc ccgacctggc
     1741 cgaatcgatc cccccctccg tccatggtcg gagctacgtg tcaaggatga gtaaaacaag
     1801 gcattgtggc cgagaaaggg tggaacgacg gcggttggcg gggcaatcgg ccggcctttg
     1861 gccgcatggg agatacggaa ggtgtgcatt gcgatagggc cggaattgtg gtttgagagg
     1921 ccaggattcc cctgaggtcc cgccacgcgc gccgaggagc ccaccgagga gtgtcgcgct
     1981 caatgcgggc agcgactccg agcggtggag cccgcttggg agcttcattc gaaggagcaa
     2041 tgccgacgat gttggtgctc aagcggttga ccgtattggc gataacgatg gtcgccatcc
     2101 tgaccggcgc ggagtcggct ctggcggggc tcgggcagcc ctcgccttgg caggtaggtc
     2161 tgcagcaatc ggcgtctccg gtgatggata acatcatctg gttccacgat ttcctgctct
     2221 acatcatcag cggtatcgcc ggtttcgtgc tggttttgct ggtcgtcgtc atggtgcgtt
     2281 tcaatgcccg caccaatccg agtccgtcgc ggaccacgca caacacgctg atcgagatcg
     2341 cgtggacgct cattccgatc gtggttctga tggttattgc ggtcccttca ttcaagcttc
     2401 tgttcctcca actcaacgtc ccggccgccg acctgaccgt gaaggcgacc ggcaaacagt
     2461 ggtactggag ctacagctat cccgacaatg gccagttcga gttcgattcc ttgatgctca
     2521 aagagggtga gcgcaaggag ggccagccgc gactgctcgc ggtcgacaac gaaatggttg
     2581 tgccggtgaa caagaccgtg cgcgtgatca ccaccggctc cgacgttatc cattcgttcg
     2641 cggtgccgtc gttcggcatc aagatcgacg ccgttcccgg gcgcatcaac gagacttggt
     2701 tcacggcaac gcgggagggt gtctactacg gtcagtgctc ggagctatgc ggcaaggatc
     2761 acgccttcat gccgatcgca gtccatgtgg tgagcgagca ggcattctcc gcctgggtcg
     2821 aagaggcgaa gaagaaatac gcgcgggatg aagcactgcc gccgaccacg gtcgcggcgg
     2881 cacaatcgac gccccggtga tgccgcgcgc agacgcggat ggtccgcggg cgacaactcg
     2941 aaggacttga gtcatggctg cgatggcgaa agctcacggc gaggacctgc ccgatcaccc
     3001 gcatggttgg cggcgttacc tctattcgac caaccacaaa gacatcggca cgatgtatct
     3061 cgtgttcgca ctgctcggcg gattggtggg cggcgtgctg tcgatcggca tgcgactcga
     3121 gctgatgcat ccggggctgc agttcttcca cgactcccac atgttcaacg ttttcacgac
     3181 tgcgcacggc ctgatcatga tcttcttcat ggtcatgccg gcgatgatcg gcggcttcgg
     3241 caattggatg ataccgctga tgatcggggc gccggacatg gcgttcccgc gcatgaacaa
     3301 catctcgttc tggctgctgc cggcatcctt cacgctgctg atcatctcgc tgttcgtgga
     3361 gggcgagccc ggtgcccccg gggtggccgc aggatggacg atctatgctc cgctctcgac
     3421 cacgggccat ccgggacccg ctgtcgattt cgccatcctg tcgctgcacc ttgccggcgc
     3481 gtcatcgatc cttggcgcga tcaacttcat cacgacgatc ttcaatatgc gcgctcccgg
     3541 catgacgatg cacaagatgc cgctgttcgt atggtcgcag ctggtgacgg tgttcctcat
     3601 tttgctcgcg atgccggtgc tcgccggtgc catcaccatg ctgctgaccg accgcaattt
     3661 cggcaccacc ttcttcacgc ccggcggggg tggcgacccg ctcctgttcc agcacctgtt
     3721 ctggttcttc gggcatcccg aggtctacat cctgatcgtg ccgggcttcg gcatgatcag
     3781 tcagatcgtg gcgacgttcg caaaaaaacc ggtgttcggt tatctaggca tggcctacgc
     3841 gatggtggcg atcggcggca tcgggttcgt ggtgtgggcg caccacatgt acaccgtcgg
     3901 catgtcgacc gccgcgcagg cctatttcgt ggtcgccacc atggtgatcg cggtgccgac
     3961 cggcgtaaag atcttctctt ggatcgccac gatgtggggc ggctcgctcg agttcaagac
     4021 gccgctgctg tgggcgatcg gtttcatctt tctattcacc gtcggcggcg tgactggtgt
     4081 ggtgctcgcc aatgccggcg tcgaccgttc cctgcaagac acctattacg tggtggcgca
     4141 tttccactac gtgctctcgc tcggcgcggt tttcacggtc ttcgccggct ggtactactg
     4201 gttcccgaaa atgtccggct atatgtactc ggagacgatc ggcaagttgc atttctggct
     4261 caccttcatt ggggtcaaca tcgtcttctt cccccagcat ttccttgggc tcgccggtat
     4321 gccgcggcgc attgctgact atcccgatgc tttcgccggc tggaacctgg tctcgtcgct
     4381 cggctcctac ctttcggcgt tgggggcgct ggtgttcttc ttcggcgtag cccatgcgtt
     4441 catgcgcaag gaaagggcgg cggacaatcc gtggggcgcg ggcgcgacca cgctcgaatg
     4501 gacgctgcct tcgccgccac cgttccacca atataacgag ctgccgctga tcaagtgatg
     4561 gcggcggcat ccccgcaggc gttcgctaac ggatgagtgc agaatagatg tcactggtca
     4621 gcgaaggtgc agagttcgcg ggacgtaggg tggcttcggc cggctacggg cccagcatcg
     4681 cgagcgtcgg tgactatgtg gcgctgatga agccgcgggt catgtctctc gtcgtcttta
     4741 ccgcgctggt cggtgtcatg atcgcgccgg gacatatcca tccggtgatc ggcttcaccg
     4801 cccttctgtg tatcgcggtc ggcgccggtg cggcgggcgc gctcaacatg tggtacgacg
     4861 ccgacatcga cgcggtgatg aggcgcacct cgaatcgtcc tattccacgg ggccgcatcg
     4921 cgcccggcga ggcgcttgcg ttcggcatcg tgctcgccgc cttcgcggta ggagtgctcg
     4981 gcctgctcgt gagctggctc gcggcggcat tgcttgcctt cacgatcttc ttttacgtcg
     5041 ctgtctatac ggcatggcta aagcgatcga cgccgcagaa tatcgttatc ggcggcgccg
     5101 cgggcgcctt tcctcccatc atcggctggg tggcggcgac cggcggcatc tcggtcgagc
     5161 cgtgtctcct cttcctcatc atcttcttct ggacgccgcc acatttctgg gcgctgtcgc
     5221 tcgttcgcgt cgacgactac gcccgcgctc gggttccgat gcttccggtg gttgccgggc
     5281 cggaggaaac gcggcggaag atattgctct attccatcgg actcgtgccg attggcgcgg
     5341 cgccgtggct tctcggctat gcccagataa tctacggcgt gagcgcgctc ctcgccggcg
     5401 cgctgatggt cgcgttcgct tggcgcgtgc acagggagcg tgacggcgag cgcgccgagc
     5461 gcgcggcgca cgatctcttc gctttctcca tcctctatct attcgtcctc ttcgcggtgt
     5521 tgctggtcga gggcggcgtg ataggccacg cggtgtgagg gctttggtga catggacgaa
     5581 aagccgaagg gaattgtcct gagcgaagag cagaagcgca gccgacgcgc gcggtcgatc
     5641 gcgattgcgc tcgcgctcgg cgcgctcgtc gtcctgtttt acgtggtcac cctggtgaag
     5701 ggaccggcgg tgctgaatcg tccgttgtaa tcgcgatgaa ggttccgaac atctcctcgc
     5761 aacgacgccg cgatgtcatg gtcgcggccg cgtgtggagt gttcgttgca gcaatggtgg
     5821 gcgcagccta tgcggccgta ccgctctaca actggttctg ccgcaccacc ggcttcggcg
     5881 gcaccaccca ggtcgcgacc gccgcgcctt cccacgtcct cgatcgcaag ctcaaggtgc
     5941 gcttcgacgc caacgtcgcc ggtggactcc cgtggcgttt cgagcctgat caaaattcga
     6001 tagtggtcaa aatcggcgag gtcgtgactg tcagctacag ggtcgtcaat gaatcggcgc
     6061 gcgagaccgt gggggttgct tcctacaatg tttcaccgcc caccgcgggc gcctatttct
     6121 ccaagatcaa ctgtttctgt tttaccgatc agcggctcaa ggccggcgaa aagcgcgaca
     6181 tgacagtcgt cttcttcgtg gcccccgagc tcgcgaagga tttcgagcag gacggtctcg
     6241 acaccatcac cctgtcctac accatgtatc cggcccggca ggctgagacg ccgcaagtgg
     6301 acaacggcgc ggcgctcgcg ccgggacgaa gctgaaccca aacagggaca agtgccgtgg
     6361 gcggcgctgc ctcaccgaga gatggagatt cctaaagatg gccgaagctc acgccaagcc
     6421 ccatcacgac taccatctgg ttgcaccgag tccttggccc gcaatcggcg cggtctccgc
     6481 attcttagcg gcagtcggcg gcattacgtg gatgcaccac atgttcgcgg ccgcgcccta
     6541 tattttcggc gttggtgtga tcggcatcgc ctatacgatg atcggctggt ggaccgacgt
     6601 cgcccgtgaa gcgacgtttg agggctatca tacgcggatc gtgcagatct ctcatcgcta
     6661 cgggatgatt ctgttcatcg cctcagaggt gatgttcttc gtggcgtggt tctgggcgta
     6721 tttcaacagc gcgctgttcc cggccgacat gcaccagatc acgcgcgaac aactgttcgg
     6781 cggggtgtgg ccgccgaagg gaaccgagac cttcgacccg tggcacttgc cgctcctcaa
     6841 taccctgatc ctgctcactt ccggcacgac cgtgacctgg gcgcaccacg cgcttctgga
     6901 gaacgaccgc cagggcctca agtggggtct gatcctgacg atcgtacttg gtctggcatt
     6961 cacctgcgtg caggcctacg aatatagcca cgcgggattc aatttcagcg gcaacattta
     7021 cggcgccact ttcttcatgg cgaccggttt ccatggcgca cacgtcatca tcggctcgac
     7081 gtttctgatc gtgtgcctgc ttcgtgtcta tctcggacat ttcacgccga cgcagcatct
     7141 cggcttcgag ttcgccgcgt ggtactggca tttcgtcgac gtcgtctgga tcttcctgtt
     7201 ttcctgcatt tatgtgtggg gttcaggcgg ggcgagcgtc gcgcatgctg ccggccacta
     7261 gagtgcgcgg tctcgatgcc gtgagtggcc tttctaattt ttggcttgcc caaatcggcg
     7321 gagacgctgg caaggtcgtt tcgggttggc ttggctcggc caccagtatc ccgacggctt
     7381 gaacgcgcgt tgaggacgga gcgtaaggcg acagcgtctt tagcgatcgc acaaagctat
     7441 tgagggagcg gcattgccga tcccatcatc atcgccgagt tgtgaacgct cttgtggacc
     7501 acaggctggc ggtgtccgct atgcttgggg atatcggctt cgggtgacaa gagccgcgaa
     7561 atgagaggtc ggttatggcg gatcatgatg cgatcattat cggcaccgga caggccggac
     7621 cggccttggc gcggcggcta gtggcagcag gttggaaggt tgc
//
LOCUS       DATLJT010001073         6608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1453419,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001073 DATLJT010000000
VERSION     DATLJT010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453419"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..264)
                     /locus_tag="VM910_24735"
     CDS             complement(10..264)
                     /locus_tag="VM910_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65762.1"
                     /translation="MRAPQEEIVRDHAYVDKAKVSPARIFKSPMDVVETNDIAPAAKL
                     AILQAWEADERALLRAEDEGMGGGEHAHLHKVQAALNHLR"
     gene            363..1259
                     /locus_tag="VM910_24740"
     CDS             363..1259
                     /locus_tag="VM910_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI65763.1"
                     /translation="MPDKKRTARLDWEDVRYFVALARHGTLSATARALRVNHATVARR
                     IASLEALIGRALFDRRARGYVLTAEGKAVLKEATAMDEAALSVLRRLDANTELSGLVR
                     LAAGRVLAERFLIDRLRAFHERYPAIDLEVIGGSRVVSLAKREADVALRYGTPKDSEL
                     VARRVATITFGLYASSSYRDKLKAGQPPAFIGFAEESDFIAEAKWLAGQFGNQRFSFR
                     TNSQTTQAAAARADYGIALLPRYIVATHEPDLVEVTLGGRLPERDVWLLVRRDLKDVP
                     RVKAVTDYLVEVFQRERRLLAG"
     gene            complement(1347..2564)
                     /locus_tag="VM910_24745"
     CDS             complement(1347..2564)
                     /locus_tag="VM910_24745"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HVI65764.1"
                     /translation="MTKVDLRRVDVARAAGASDVSRAAGRVRLDSIDLLRGLVMVLMA
                     LDHTRDFFAAGGPNPRDIADPALFLTRWVTHFCAPTFIFLAGVSAFLYGERGRSVGQV
                     SLFLLTRGLWLILIEFTLMRFCWTFSFHINVFVLQVIWVTGASMVVLAGLVHLPRGAI
                     AAIGLAMIAGHNLLDGIDAADLGSAAWLWTLLHQPGLIEIAPQLKLFVLYNLVPWSGV
                     MAASYALGPVYRLDPAARRSFLVRTGAAVTAGFVVLRATNLYGDPDAWSMQSTVLGTV
                     LSLIDCEKYPPSLLYLMMTLGPALILLALFERAHGAIAGVVTTFGRVPFFFYAVHVAF
                     IHALAVGFAWITVGDIGWTFGSFLSQKPASYGLNLLGIYAVWLCVVAALYLPCRAFAA
                     LKQRRREWWWSYL"
     gene            complement(2717..3727)
                     /locus_tag="VM910_24750"
     CDS             complement(2717..3727)
                     /locus_tag="VM910_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI65765.1"
                     /translation="MGIEALGYIGVRAKDVGDWASYGENVLGLQRIDKSRSSLAFRMD
                     DRKQRLVVDADGGQGISFFGWEVADAAALDAVAARIEAAGTKVARGSRALAGERHVED
                     LIVLNDPAGNRLEIFHGGETTADPFKPGRNISGFRTGPLGMGHVVMHFERITEVMAFY
                     QNVLEFKVSDYWLRPFPAYFFHVNPRHHSIAFVESGINMVHHMMVELYSFDDVGQGYD
                     LALGDPERIGVTLGRHSGDYVTSFYTWNPSGFLVEYGWGGQLIDDTTWKPFERKFGPS
                     LWGHERRWMSAEKKEESRQLCIEAAENGQRQPVQVIAGNYNLMPGVCPWWDSMKQGRK
                     AG"
     gene            4402..5604
                     /locus_tag="VM910_24755"
     CDS             4402..5604
                     /locus_tag="VM910_24755"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI65766.1"
                     /translation="MKSLYLLLSLDPASLALLFWFTLAFDVPRYVISVIVISIFERRK
                     LPPLRLTTSAIVAGYNEVHSIRACVESINADQIIVVDDGSTDGMWKVVEQLHAEGLVH
                     KAIRLPVRNSKITAMNIGLEACTGEIVFLVDADTILEPGAIDAALPYFADPKVAGVSC
                     DLKVDNESASLTTRFQAIEYAIALSVGRQVGDALGLMPLVSGACGAFRRSALREVGGL
                     DMEVCEDAALTMKLRGAGYKIRFAPEAIGKTKVPETLTALTVQRFRWDAALITVWCRR
                     CIGNISPLSPHFSLIEALVILDVIWFSIILPLALPIYCVWLWSNVGEFAFTLLGAVFI
                     GLTALDVFMCILARVPLRLFPYIPLYTLMQNIVMRPIRIVALLGELIFVNSRRDSFIP
                     EQQRWRLS"
     gene            5610..6527
                     /locus_tag="VM910_24760"
     CDS             5610..6527
                     /locus_tag="VM910_24760"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVI65767.1"
                     /translation="MAIKWTYLLALIGFAIWVFDVFFGGHIYLRGNGLVLGQPAVVAA
                     EYNVTVREAMVKEGDRVAEGQVVIRISSQQVAEARARLSSEAATRAARLVDMDIRSEV
                     VDAMLAPAQHREAVAIEGKNQLNESYKKGLLAVINRTAAAEQAYNGQKDAEALRAEKR
                     VLNDQIKMLTIATEQADLALSDLLDLFDQGRLRAPIAGTVSAVLANRGAVVRAGDPLL
                     ELVGDHRFVVAWVPVGRWYKLEVGQRVSIDAGTGGLPGTISRIGTVALALPREFQKAF
                     TPTERLQLIWIEFEQGVTAPPYFTKVVIN"
BASE COUNT         1331 a         1901 c         2072 g         1304 t
ORIGIN      
        1 gccggccgct tacctgagat ggttgagggc agcttgcacc ttgtgcagat gcgcgtgctc
       61 tcctccaccc atgccttcgt cttcggcgcg cagaagcgct cgttcatcgg cctcccacgc
      121 ctgcagaatg gccagcttcg ccgccggcgc tatgtcgttc gtttcgacga catccatggg
      181 ggatttgaat atacgcgcag ggctcacttt cgccttgtcc acataagcat gatctcgcac
      241 gatttcctcc tgtggggctc tcatttcgcc ctccggagac ggtccggcag ggcatccgat
      301 cgtacgtttg gatcaatatc taggagttgg cagcctgggc gataatccta tatttcttcg
      361 ccatgcctga taaaaaacgc acagccaggc tggactggga agacgtccgt tatttcgtgg
      421 cgctcgcccg ccacggcacg ctttccgcga ccgcgcgcgc gttgcgcgta aatcacgcaa
      481 ccgtggcgcg ccgtatcgca agcctggaag cgctgattgg ccgcgccttg ttcgaccggc
      541 gcgccagggg ctacgtgctg actgcagaag gcaaagccgt gctcaaagag gcgactgcca
      601 tggacgaagc ggcgctttct gttctgcgcc gtctcgatgc taacacggaa ttgagcggcc
      661 tcgtccgcct cgccgccggc cgcgtgctgg ccgagcgctt tctcatcgac cgtctgcgcg
      721 cttttcatga acgttatccg gcgatcgacc tagaggtgat cggcgggtct cgggtcgtga
      781 gtcttgcgaa acgggaagca gatgtcgcgc tgcgatacgg gacgcccaag gacagtgagc
      841 ttgttgcccg acgcgtggca acgatcacct tcggccttta cgcatcgtcc agctatcgcg
      901 acaagctcaa ggccgggcaa ccgccggctt tcatcggttt tgccgaagag agcgacttca
      961 tcgccgaggc gaagtggctg gccggccagt tcggcaacca gcggttttca tttcgcacta
     1021 acagccagac aacgcaggcg gcagcggctc gcgcggatta tggcattgcg cttcttccaa
     1081 gatacatcgt ggcgacgcat gagccggatc tcgtggaggt gacgctggga ggacgtttac
     1141 cggagcgcga tgtgtggctt ttggtccgcc gtgatctaaa agacgttccc cgagtgaagg
     1201 ccgtgaccga ctatcttgtt gaggtgttcc agcgcgagcg acgactgttg gcaggttagc
     1261 tagctgttgc agaaggcgga ttatgagtac acgcctgaga acgttttcgg tcctggtctc
     1321 gcgcgctcgc gcggcagtcc atctcctcag agatagctcc accaccattc gcgccgccgc
     1381 tgcttgagcg ctgcaaaggc gcggcagggc agataaagcg ccgccaccac gcagagccag
     1441 acggcgtaga tgccgaggag gttgagcccg tagctcgcgg gcttttgcga gagaaacgag
     1501 ccgaacgtcc agccgatgtc gcccacggtg atccaggcga agcccacggc aagcgcatgg
     1561 atgaatgcga cgtgcaccgc gtagaagaag aacggcacgc ggccgaacgt ggtgacgacg
     1621 ccggcgatcg cgccatgcgc gcgctcgaac agcgcgagca ggatcagcgc gggtccgagc
     1681 gtcatcatca gatagagcag cgacggcgga tacttttcgc aatcaatgag cgacagcacc
     1741 gtgccgagga cagtgctttg catgctccac gcgtccgggt cgccgtaaag attggtggcg
     1801 cgcagcacga cgaaccccgc cgtgacggcg gcgccggtgc ggacgaggaa ggaccggcgc
     1861 gccgccggat cgagccggta caccggaccc aaggcgtagc tcgccgccat cacccccgac
     1921 caaggcacga ggttataaag cacgaacagc ttgagctgcg gggcgatctc gatcagcccc
     1981 ggctgatgca gcagcgtcca cagccaggcg gcgctgccga gatccgcggc atcgatgccg
     2041 tcgagcaggt tgtgtcccgc gatcatggca agcccgatcg cggcgatcgc cccgcgcggc
     2101 aggtgcacga gtcccgccag caccaccatc gacgcgccgg tgacccagat cacctgcagg
     2161 acgaaaacgt tgatgtggaa gctgaaggtc cagcagaagc gcatcagcgt gaactcgatc
     2221 aggatgagcc acaggccgcg cgtcagcaga aataagctga cttggccgac gctgcgtcca
     2281 cgttcgccat aaaggaaggc cgaaacgccg gcgaggaaga tgaaggtcgg cgcgcaaaag
     2341 tgcgtcaccc agcgcgtcaa gaacaacgcg ggatcggcaa tgtcgcgcgg gttggggccg
     2401 cccgcggcga agaaatcgcg ggtgtggtcg agcgccatca gcaccatgac gagcccgcgc
     2461 aagagatcga tgctgtcgag acgcacgcgg cccgcggcgc gcgacacgtc gctggcgccc
     2521 gcggcgcggg caacgtccac acgccgcaga tcaactttgg tcatgcaatc gttccttggg
     2581 tagtgtcccg atgcgcaggc agtcgtgcgc gagaagtctg caccgacgcg cgcaaccgtc
     2641 gatttgaaat gatgtgccga gtgggtgggg gcgctgcctc aaccgccgcg acacgaggcc
     2701 gagcgaagaa agacgatcac cccgccttgc ggccctgctt catgctgtcc caccaggggc
     2761 acacgccggg catgagattg taattgcccg cgatcacctg caccggctgg cgctggccgt
     2821 tctcggcggc ctcgatgcag agctggcgcg attcctcctt cttttccgcc gacatccagc
     2881 gccgctcgtg cccccacagg ctcgggccga atttgcgctc gaacggcttc caggtggtgt
     2941 cgtcgatgag ctggccgccc cagccgtact cgaccaaaaa gcccgacggg ttccaggtgt
     3001 agaacgaggt gacgtaatcg ccggaatgac ggccgagcgt gacgccgatg cgctcggggt
     3061 cgccgagcgc gagatcatag ccttggccca catcgtcgaa actgtagagc tccaccatca
     3121 tgtgatggac catgttgatg ccgctctcga cgaaggcgat gctgtgatgg cgcggattga
     3181 cgtggaagaa ataagccggg aacgggcgca gccagtagtc gctgaccttg aattcgagga
     3241 cgttctgata gaacgccatc acctcggtga tgcgctcgaa atgcatcacc acgtggccca
     3301 tgccgagcgg gccggtgcgg aagccggaaa tgttgcggcc gggcttgaac ggatcggcgg
     3361 tggtctctcc gccgtggaag atttcgagcc ggttgccggc gggatcgttg agcacgatca
     3421 agtcttcgac gtggcgctcg ccggcgagtg cgcgcgagcc gcgcgcgacc ttggtgccgg
     3481 ccgcctcgat gcgcgccgcc acggcgtcaa gcgccgcggc gtcggcgacc tcccagccga
     3541 agaagctgat gccctgcccg ccgtcggcat cgaccacgag gcgctgcttg cgatcatcca
     3601 tgcggaaagc gaggctcgag cggctcttgt cgatgcgctg caggccgagg acgttttcgc
     3661 cgtagctcgc ccagtcgccc acgtccttgg cgcgaacccc gatatagccc aacgcttcga
     3721 tgcccatgat ggccctcgct cattgccgcc cgacacgggg tgggcgcgcc cgcacgaaac
     3781 cgcgggattc ctggatttgc gtcgccaaca actaggagtt cttggactcc acagaatacg
     3841 cgtttaccga gaggctcggg cactaaactg cctgcctgat ctgttattta gcaaaaaaac
     3901 ttcctggatt ggaaccgtaa gccggtcggt ttcgttttag tgccggtacg aaggtactct
     3961 cacgcgacaa tcatagcgac tcccatgaaa catgctttga tcacagcatc tgttgtggcg
     4021 ctcgtggctg gaattggctt ggcatgtgcg caggaaatga acgagcgccg ggaagcgccg
     4081 gccgccacgc cggagcagaa ggcgccggaa ggccagacgg gcctgcagag gccttctgcg
     4141 ccgccgatgt cgcagaaggc aaaacggtct ccaaccgcgc aggactcaga agcgaccggc
     4201 tggggctccc cggccggagg ggggtcgtcg ggtgctgcgg ccagcgcggc agcacaggag
     4261 gagcgcagga ctgccgcgga ggtccggcgg gtccgtgccg cggtcgagcg aacgctgcgc
     4321 cgaacgcagg caatgttccg aacggcttcg cactgatgca agcttgaggg gtcggctgac
     4381 tgaaactggg gggcagggga cgtgaaatcg ctttatcttt tactctccct cgatccagcg
     4441 tcgttggctc tcctattttg gttcacactg gcgttcgacg tgccgcggta cgtcattagc
     4501 gtcatcgtga tttccatttt tgaaagaagg aagctgccgc cacttcggct gacaaccagc
     4561 gctatcgtgg ccggatacaa tgaagttcat tccatccggg cttgtgtcga atcgataaac
     4621 gccgatcaaa tcatcgtagt cgatgatgga tcgacggacg gtatgtggaa agtcgttgag
     4681 caattgcatg ccgaaggttt ggtccacaaa gctattcggc ttccagtgag aaacagtaaa
     4741 attacggcca tgaatatcgg attggaagca tgcacgggcg aaattgtatt tcttgtcgac
     4801 gccgacacga tactggagcc tggcgccatt gatgcagcgt tgccgtattt cgctgatccg
     4861 aaagtcgcag gcgtaagctg cgacctcaaa gtcgacaacg aaagcgcgag cctcacgacc
     4921 cgttttcaag ccattgaata tgcaattgcc ctttcggtgg gtcgccaagt cggcgacgcg
     4981 ttggggctca tgccgctggt atctggagca tgtggagctt ttcggcgttc cgcgcttcga
     5041 gaagttggtg ggctggatat ggaggtttgc gaagacgcgg ccctgacgat gaaattgcgt
     5101 ggcgcaggat acaaaattcg gtttgcgccc gaagccattg gtaaaaccaa ggttccagag
     5161 actctgaccg ctttgacggt gcaaaggttt cgatgggatg ccgcgttgat taccgtttgg
     5221 tgcagaaggt gcattggaaa catcagtcca ttatcacccc attttagtct tattgaagct
     5281 ctggtgatct tggatgtgat ttggttttca atcattttgc cccttgcatt gccaatttat
     5341 tgcgtctggc tgtggagcaa tgtaggggaa tttgctttca cattgttggg cgcagttttc
     5401 atcgggctta cggccttgga tgtttttatg tgcatcctag cacgcgtgcc actcaggctg
     5461 tttccgtaca tccctctgta tacgttgatg caaaacatcg tcatgcgtcc catcagaata
     5521 gtggcgctcc ttggcgagct catttttgtg aactcgcggc gcgacagctt tattccagag
     5581 caacaaaggt ggaggctatc gtgaagcgca tggcgatcaa atggacctac cttttggcgc
     5641 tgatcgggtt cgcgatctgg gtctttgatg ttttcttcgg cggacacatc tacctgcgcg
     5701 gcaacggctt ggtgctcggg caaccagctg ttgtcgccgc cgaatataac gtcaccgttc
     5761 gagaggcgat ggtaaaggaa ggcgaccgag ttgccgaagg acaggtggtt atacggatat
     5821 cttcgcagca agtggctgag gctcgcgcca gactgagctc cgaagcagct acgcgggcag
     5881 ctcgactagt tgacatggat atccgtagcg aagtggtgga tgcgatgtta gcgccggcgc
     5941 aacatcgtga agcggtcgct attgaaggaa agaaccagct caacgaaagc tacaagaagg
     6001 gacttcttgc agttattaat cgcaccgcgg ctgcggagca ggcctataat ggccaaaagg
     6061 atgcggaagc attgcgcgcc gaaaaacgag tgctgaatga tcagatcaag atgctgacga
     6121 tcgccaccga gcaggccgat ttggccctgt cggatcttct cgacctgttc gaccaaggaa
     6181 ggctacgtgc gcccatagcg ggtaccgtta gcgcagtcct ggccaatcgt ggcgctgtcg
     6241 tgcgagctgg cgatccgttg ttggagttgg ttggcgatca ccgatttgtt gtcgcttggg
     6301 ttccagttgg aagatggtac aaattggaag tcggtcaaag ggtctctatt gatgcgggga
     6361 ccggagggct acccggaaca atttcaagga ttggtacggt ggcgttggcc ttgccaagag
     6421 agtttcaaaa ggcttttacg ccgaccgaac gcctccaatt gatttggata gaatttgagc
     6481 aaggcgtcac agcgcctccg tacttcacca aggtcgtaat caactaacgc cgtcccaagt
     6541 cgcaaacctc cccgagttcg gcctttcaga tcgcgagatt tgcaagcgtg cgacagtaag
     6601 cgcgcaga
//
LOCUS       DATLJT010001074         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3291745,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001074 DATLJT010000000
VERSION     DATLJT010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291745"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VM910_24765"
     CDS             complement(<1..332)
                     /locus_tag="VM910_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65768.1"
                     /translation="MAALTNPGLPGSFLFDTVIYASAGSNYGGVPALSQYTIYDTPDP
                     AYVTPRINISVFANLGIGAPWPFPNTSVPLNGHVCIPRGRGPFPLAVFAHGNHDPLSN
                     STPGYLYL"
     gene            complement(968..1231)
                     /locus_tag="VM910_24770"
     CDS             complement(968..1231)
                     /locus_tag="VM910_24770"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI65769.1"
                     /translation="MGSCPSDISKERLARRAARGAAGQPVDATERLRDRADALSARAA
                     AVRRLADAEKPLYQSLDEAQKRRFAIALRFAARRHMAEHHPHG"
     gene            complement(1424..1864)
                     /locus_tag="VM910_24775"
     CDS             complement(1424..1864)
                     /locus_tag="VM910_24775"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase C-terminal domain-containing
                     protein"
                     /protein_id="HVI65770.1"
                     /translation="MLIPPTTKPGNSRHGRSSNSGVNPDTLRAMTLDVVFDYLGTRLN
                     ASRAGTTQTVINWRFPDTKETAASTLSHGALTAVVGKDAASADATVTIPRSVFAAAVL
                     GERSIADEIRRGEAVVDGKAAVVTQLFGLFDDFDTAFPIVEPRR"
     gene            complement(2450..>2683)
                     /locus_tag="VM910_24780"
     CDS             complement(2450..>2683)
                     /locus_tag="VM910_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI65771.1"
                     /translation="GEAGWRDVGKGPGSIDGKFIKWHTISDLAGSVVEDVVRIRNHPL
                     VAKNITIYGYIYDVNNGRLVEVNEATKAGQAAA"
BASE COUNT          503 a          857 c          824 g          499 t
ORIGIN      
        1 cacaggtaca gatagcccgg cgtcgaattc gacagaggat cgtgattgcc gtgcgcgaac
       61 acggcgagcg ggaacggccc gcgtccgcgc gggatgcaga catgtccgtt gagcggcacg
      121 ctcgtgttcg ggaagggcca cggggcgccg atgcccaggt tggcgaacac cgagatgttg
      181 atccgcggcg tgacgtaggc ggggtccggc gtgtcgtaga tcgtgtactg ggacaacgcc
      241 ggcacgcccc cgtagttgct gcccgccgag gcgtagatca ccgtgtcgaa caaaaagctg
      301 cctggaagcc caggattggt cagcgctgcc atgggcgctc ctttgcgcag acgtggatcg
      361 aagcctagtc tagcggccgc gccacgcaaa atgaatttcc gtcagggcga aagccgattc
      421 ggcgcaccga ccggtgtcgt tcccgaaaat tgtcagcaaa tacatgattc ggcgccgcgg
      481 gccgccatcg cagagaccct ggggaacttt catacgcagc catgcggacg cgcacgatgg
      541 gcaacgcatc cccagcaaat acgacgccta tctcggaacg gagctgatcg ttcgggcgag
      601 cgccacgccc ctgctcgcgg ccgcgcgcgc tttagtcgcg ctaaccgtgt agaatgtcgt
      661 gcattttttg aggtcccgta tatgagccac agacgcaaat tatgcctttg aaagatcgca
      721 cagatttgcg acgccgctcg gccgcgccga cgaggtgatc gagcaaatca ttcaaacccg
      781 tgcacgtcgt atcttgccgc gcatgagtcc ggttttggta cgaaccggac ctgccaaccg
      841 ccgcgtttga tttccgtttt ctagccgtcc cgggaaagtg tctgcgacgg tggtgagcgc
      901 ccccgccccg agcgattagc gcggcggggt agcaagactg cgatcagcga gcgtgttcgg
      961 acgcgcctca gccgtgcggg tgatgctctg ccatgtgccg acgcgcggcg aaacgcaaag
     1021 cgatggcgaa acggcgcttc tgcgcctcgt cgaggctctg gtacagcggc ttctcggcat
     1081 cggccaagcg ccgcaccgcc gccgcccgag cgctcaacgc atccgcacga tcgcgcaggc
     1141 gctcggttgc atcgacaggc tgcccggcag cgcctcgcgc cgcgcgtcgc gcgagccgct
     1201 ccttgctgat gtccgagggg catgaaccga ttgcgcaaga tcaacaccaa ctagtgcgcg
     1261 tgctcgtccg ctcgatgcca atggcccgct ggctattaat gcaatagcaa gttggcatcg
     1321 accgattgat cgggacgact gtacgaagag accggttctt ttgaaagtgt gccatcgctt
     1381 tcgaaggagg cttgctccca tgacccacac atggcactca cagctagcgg cggggctcca
     1441 cgatgggaaa ggcggtgtcg aaatcgtcga acagtccgaa gagctgagtc acaacggctg
     1501 ccttaccgtc cacaaccgct tctccgcgcc ggatctcgtc ggcgatggaa cgctccccaa
     1561 gcacggccgc cgcgaacacc gaccggggta tggttactgt ggcatcggcg ctcgcagcgt
     1621 cctttcccac cacggcagtc agcgccccat gcgacagcgt cgaggctgcg gtttccttag
     1681 tgtcggggaa gcgccagttg atcacggtct gcgtggttcc cgcacgcgac gcgttcaggc
     1741 gtgtgcccaa gtaatcgaag acaacatcga gcgtcatcgc acgcaaggta tccggattga
     1801 cacccgagtt gctcgaacgc ccgtgccgcg agttcccggg cttcgttgtt ggtgggatca
     1861 gcaaaaacca cttgatccat gacttgcgcg acccagcggt agttgccggc tttgaagtcg
     1921 tcgcgggcgc gcgcgatcac cggcgccgct ccgcccatgt attcaacata cttcttcgcg
     1981 ttgcgggtcg ctttcccact tctcgttcgc gcaccacgga gccagccccc atcagtgacg
     2041 ataatcagcg caaccttgat catcgtttgc tcggcttgct tcatttccgc accaccttga
     2101 ccggcggccg cacaggcggc gtcggtgtga tcgccacggc ggcaaccaga gccatgccgt
     2161 caagctgcca cgctgaacaa gctgcaaggc ttgaccgagg gcaagaggtc accacgcaag
     2221 tcggcgagcg gattagttct cagagactcc gtggtgtctc gttaagccgc gggccaagac
     2281 tctcagtccg gcgagcaggt ctcgaacttg cgcatctcga attaaaggcg cgcccgctcc
     2341 gccgcctgac aggcgaatcc ctggccattt cgggaagccg ggagctacga ccggcggtgg
     2401 atatattagg gcacgtcggg ctgcgtccgg tcgacagcaa cggcgcccgt caggcggcgg
     2461 cttgtccggc cttagtcgcc tcattcacct ccaccaagcg gccgttgttc acatcgtaaa
     2521 tgtagccgta gatcgtaatg ttcttcgcca ccaatggatg attgcggatg cgtacaacgt
     2581 cctcaaccac gcttccggcg aggtcgctga tcgtgtgcca tttgatgaac ttgccgtcga
     2641 tcgagcccgg gcccttgccg acgtcgcgcc agccggcttc gcc
//
LOCUS       DATLJT010001075         4527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3291803,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001075 DATLJT010000000
VERSION     DATLJT010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291803"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VM910_24785"
     CDS             <1..1077
                     /locus_tag="VM910_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVI65772.1"
                     /translation="CEAGVILQVAQQRAAEVDRLFPLSLGAEGSCTIGGNLSTNAGGT
                     TALAYGVAREMALGVEVVLADGRVLNLLSKLKKDNTGYDLRNLFIGAEGTLGIITAAT
                     LKLFPKPRAIETAFVGLTSPAQALKLLSLSQNEAAGTLTSFELLSQTAVDFSIRHGID
                     VRDPLSAKRPWYVLMELSSPRDDARVTLEAILAKSLEDGIVDDAVIAANLSQRQAFWK
                     LRDEMSAAQKPEGGSIKHDISVPVAAVPDFIAEADAAVVKLIPGSRPVPFGHLGDGNI
                     HYNISQPVGADAADFLARWHEVNAVVFDIVLKMGGSISAEHGIGVLKREELPDVKDKT
                     AIELMRAVKALLDPNGIMNPGKVL"
     gene            1074..1535
                     /locus_tag="VM910_24790"
     CDS             1074..1535
                     /locus_tag="VM910_24790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HVI65773.1"
                     /translation="MTEPPSVPPAALAIAPIGEADIASVATLWQRCGLTRPWNDPQAD
                     IALARRSPNATILVGRDGNGIVASAMVGHDGHRGWVYYVAVDPDRRAKGFGRAIMSAA
                     EDFLRQTGIAKLQLMVRPENDEVRAFYESIGYDEQERVIYAKWLDGREMTP"
     gene            1554..2135
                     /locus_tag="VM910_24795"
     CDS             1554..2135
                     /locus_tag="VM910_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase"
                     /protein_id="HVI65774.1"
                     /translation="MSLSDRIRVKNVRLLSDDHYILKTTAFEWRRADGEWQTQHRETY
                     DRGNAATVLAYNLARRTVVLVKQFRYPAYVNGYDDLMIEAAAGLLDGEEPEVRIRAEA
                     EEEIGYRLGAIRKIFEAFMSPGAVTEKIHFFVAEYEPSMRIGTGGGLAHEGEDIEVLE
                     LSIDEAVAMIADGRIVDGKTIILLQYAALNIFK"
     gene            complement(2159..2926)
                     /locus_tag="VM910_24800"
     CDS             complement(2159..2926)
                     /locus_tag="VM910_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVI65775.1"
                     /translation="MCGRFVITSPPAALRQIFGYFEQPNFPPRHNIAPTQPVPVVIVE
                     QGQRHFRLMRWGLIPSWVKDPRTIALMINARSEGIAQKPAFKNAIRRRRCLIPADGYY
                     EWQASGSRKRPFFIYRADRRPFALAGLAETWVGPNGEELDTVAVVTTAASRDLAALHH
                     RVPVTIRNDDFARWLDCSADEAASVMELLAPPGEGEFTYHEVSTRVNHADNDDAQLIL
                     PITDEQRAAEEGSKSAKRAPSRKPPPPAVDDGQGSLF"
     gene            2991..3503
                     /locus_tag="VM910_24805"
     CDS             2991..3503
                     /locus_tag="VM910_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65776.1"
                     /translation="MTFEASPSPRHDRTTQRLTVSDADARSAELRAANINPRTGLATD
                     YLNHFNQAVMLLDMIPDMPECAEDFLQWQPLSYAEHFTASNFKARDLAISAYETADAG
                     TRAEFDHITSTMTSILTAVGDAMRETIQENTRAKLARQAARWVKPLVATAGGIINGGS
                     DADVDYIMTH"
     gene            3774..4199
                     /locus_tag="VM910_24810"
     CDS             3774..4199
                     /locus_tag="VM910_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI65777.1"
                     /translation="MPEASSAQPTHPQLAVSAVIFREGKVLLVRRARSPAKGFYSLPG
                     GRVEFGESLATALHREVREETGLKIEIVALAGWREVLPGPGGGGHYLIMSFAARWIGH
                     EPVLNEELDDFKWLVPEDIGDLKLTGGLVEVVSAARRLL"
     gene            4335..>4527
                     /locus_tag="VM910_24815"
     CDS             4335..>4527
                     /locus_tag="VM910_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HVI65778.1"
                     /translation="MSKRCLAALILIACLAGSVRAENAGDAAAPFDGDLQRLAEILGT
                     LHYLRGICGSNEGGKWRNQM"
BASE COUNT          845 a         1418 c         1423 g          841 t
ORIGIN      
        1 tgcgaagccg gcgtgatcct gcaggtcgcc cagcagcgcg ccgcggaggt cgaccggctg
       61 ttcccgctgt cgctgggggc ggagggaagc tgcaccatcg gcggaaatct ctccaccaat
      121 gccggcggca ccaccgcgct cgcctatggc gtggcgcgcg agatggcgct gggcgtcgaa
      181 gttgtgctgg ccgacgggcg cgtgctgaac ctgctgtcga aattgaaaaa ggacaacacc
      241 ggctacgacc tgcgcaacct cttcatcggc gcagagggaa cgctgggcat catcacggcc
      301 gcgacgctga aattgtttcc aaagccgcgc gcgatcgaaa ccgcctttgt cggcctcacc
      361 tcgcccgccc aggcgctgaa actgttgtcg ctgtcgcaga acgaagccgc cggcacgctg
      421 acgagtttcg agctgctctc gcagaccgcg gtggatttct cgatccgtca tggcatcgac
      481 gtgcgcgacc cgctttccgc caagcgtccc tggtatgtgc tgatggagct gtcgtcgccg
      541 cgcgacgatg cgcgcgtcac gctggaagcg attttggcga agagcttgga ggacgggatc
      601 gtcgacgatg ccgtgatcgc cgcgaatttg agccagcgcc aggcgttctg gaagctgcgc
      661 gacgagatgt cggcggcgca gaagccggag ggcggctcga tcaagcacga catctcggtg
      721 cctgtcgccg cggtgccgga tttcatcgca gaggcggatg ccgcggtggt caagctgatc
      781 ccgggctcgc ggccggtgcc gttcggccat ctcggcgacg gcaatatcca ctacaacatc
      841 agccagccgg tcggcgccga tgccgccgat ttcctcgcgc gctggcacga ggtcaacgcc
      901 gtcgtgttcg acatcgtgct gaaaatgggc ggctcgatct cggccgagca cggcatcggc
      961 gtgctcaagc gcgaggagct gcccgacgtg aaggacaaga ccgcgatcga gctgatgcgc
     1021 gccgtgaagg cgttgctcga tcccaacggc atcatgaatc ccggcaaagt attgtgaccg
     1081 aacctccttc agtgccgccc gccgcgctcg cgattgcacc gatcggggag gccgacatcg
     1141 cgtctgtcgc gacgctgtgg cagcgttgcg gcctgacgcg gccctggaac gatccgcagg
     1201 ccgacatcgc gcttgcccgc cgcagtccga acgccacgat cctggtcgga cgcgacggca
     1261 acggaatcgt ggcgtcggcg atggttggcc atgacggcca tcgcggctgg gtctattacg
     1321 tggcggtcga tcccgaccgc cgcgcaaaag gctttggccg cgccatcatg agcgcggcgg
     1381 aggattttct gcggcagacc ggcattgcaa agctgcaatt gatggtgcgg cccgaaaacg
     1441 acgaggtgcg ggccttttac gagtcgatcg gctatgacga gcaggagcgc gtcatctacg
     1501 ccaaatggct cgatggccgc gagatgaccc cctaaaccgc tggcatggta tgcatgagcc
     1561 tttccgatcg tatccgcgtc aagaatgttc gcctgctttc cgacgatcac tacattctga
     1621 agaccaccgc attcgagtgg cgtcgcgcgg acggcgaatg gcagacgcag catcgcgaga
     1681 cctatgaccg cggcaatgcc gcaaccgtgt tggcatacaa cctcgcgcgc cgcaccgtcg
     1741 tgctggtgaa gcagttccgc tatccggcct atgtcaacgg ctatgacgat ctgatgatcg
     1801 aagcggccgc gggcctgctc gacggcgaag aacccgaggt gcggattcgc gcggaggcgg
     1861 aagaagagat cgggtatcgg cttggcgcga tccggaagat cttcgaggcc ttcatgagcc
     1921 ccggcgccgt gacggagaag atccatttct tcgtggccga atacgagccc tccatgcgga
     1981 tcggcactgg cggcgggctt gcccatgagg gcgaggatat cgaggtgctc gagctttcga
     2041 tcgacgaggc ggtcgcgatg atcgccgacg ggcgcatcgt cgacggcaag acgatcatct
     2101 tgctgcagta tgcggcgctg aatattttca agtgagctct gcgggacccg atgtgtagct
     2161 agaacaaact cccctgcccg tcgtcgaccg cgggcggcgg aggcttgcgc gatggggcgc
     2221 gttttgcaga cttcgacccc tcttccgccg cgcgctgctc gtccgtaatc ggcaggatca
     2281 attgcgcgtc gtcattgtcg gcgtggttca cccgtgtcga gacttcgtga taggtgaact
     2341 ccccctcccc gggcggcgcc agcagttcca tcaccgaagc cgcttcatcg gcgctgcaat
     2401 cgagccaacg cgcgaaatcg tcgttcctga tggtcacggg cacgcggtga tgcagcgccg
     2461 cgagatcacg gcttgccgcg gtggtgacga ccgcaaccgt gtcgagttcc tcgccgttcg
     2521 gccccaccca ggtttcggcc aatcccgcca gcgcaaatgg acgccggtcg gcgcggtaga
     2581 tgaaaaacgg ccgcttccgg ctcccggacg cctgccattc gtaatagccg tcggccggaa
     2641 tcaggcagcg ccgccgccgg attgcgttct tgaatgccgg cttctgcgcg atcccctcgg
     2701 agcgcgcatt gatcatcagc gcaatggtgc ggggatcctt gacccaggac gggatcagcc
     2761 cccagcgcat cagacgaaaa tgccgctggc cttgttcgac gatcaccaca ggcacaggtt
     2821 gtgtcggtgc gatattatgc cttggcggaa aatttggctg ctcgaaatag ccaaaaattt
     2881 gccgcaaggc cgccggcggg gaggttatga cgaagcgtcc gcacattctg tgccctaaat
     2941 aagcattcgt tcagcccctg ttaaccccga aaaccctaac agtggaaacg atgacgttcg
     3001 aggcctcacc atccccgcga cacgatcgca cgacgcagcg attgacggtc tccgacgcag
     3061 acgcccgctc agccgagttg cgcgccgcca acatcaatcc gcgcaccggg cttgctaccg
     3121 actatctcaa tcacttcaac caggcggtga tgctgctcga catgattccg gatatgccgg
     3181 aatgcgccga ggatttcctg cagtggcagc cgctgtccta cgccgagcat ttcactgcct
     3241 ccaatttcaa ggcgcgcgat ctggcgatct ccgcctatga aaccgcggac gccggcaccc
     3301 gcgccgagtt cgatcatatc acgagcacca tgacctcgat tctgaccgcg gtcggcgacg
     3361 cgatgcgcga aacaatccag gaaaacaccc gcgccaagct tgcccggcag gcggcccgct
     3421 gggtcaagcc ccttgttgcg acagccggcg gcatcatcaa tgggggatcg gatgcggatg
     3481 tcgactacat catgactcac tagtgtgccg gttcggaagt cggcctcatt ctctgctttc
     3541 gcaatgcaga acttccgagc gaaagcgcga tacaatcaga aattgttagc gattgctcgg
     3601 aagttctctt catttcttcc aggggttcat caagaggact tccgaaccaa aaccccactg
     3661 gggtcataag tttgccaaga cgaacgcgac ggatggccac ctggctctgg attcctgttt
     3721 gagagcatga tctcttcgga aaatcgattt ccacttttcc ggatcatgct ctgatgcccg
     3781 aggcatcgtc cgctcagcca acccatcccc aactcgcggt gagcgccgtg atctttcgcg
     3841 agggcaaggt gctgctggta cggcgcgcgc gatcgccggc gaagggtttc tattcgttgc
     3901 ccggggggcg ggtcgaattc ggcgaatcgc tcgcgaccgc cttgcaccgc gaggtccgcg
     3961 aagagaccgg attgaagatc gaaattgttg cccttgccgg ctggcgcgag gtgctgcccg
     4021 gaccaggcgg cggcgggcat tacctgatca tgtcgttcgc cgcgcgctgg atcggccacg
     4081 agccggtgct gaacgaggaa ctcgacgact tcaaatggct ggttccggag gatatcggcg
     4141 atctgaagct gaccggcggg cttgtggaag tcgtttccgc ggcgagaagg ctgctttagt
     4201 gtcccgcttc cgaagttcgc gcaaagatgc tgcgatcgcg cgcgaacttc gaaaagtggg
     4261 caccggagtt cattttggcg ccttgcttcc agcgcagggg aagggcatac cacgcccgtc
     4321 tttcggacct ctcgatgtca aaacgctgtc tcgccgcatt gatcctgatc gcttgcctgg
     4381 ccggctccgt ccgggcggaa aatgcaggcg acgcggccgc gccgtttgac ggcgacctcc
     4441 agcgcctcgc ggaaatcctc ggcaccctgc actatctccg cggcatctgc ggcagcaatg
     4501 aaggcggcaa atggcgcaac cagatgc
//
LOCUS       DATLJT010001076         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1913531,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001076 DATLJT010000000
VERSION     DATLJT010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913531"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VM910_24820"
     CDS             complement(<1..1068)
                     /locus_tag="VM910_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVI65779.1"
                     /translation="MGELREIRIPDIGDFKNVPIIEIAVQPGQRVGVDAPLITLESDK
                     ASMEVPSPAAGVVKSLAVKVGDKVSEGASILMLEVDGAGPAEEPVKPAVSAPPAPTSA
                     PAGVADVRVPAIGDFKDVPIIEVMVKPGDTIAAEQPLVTLESDKASMEVPSPLGGKVA
                     DLKVKVGDRVSEGTVILTLITEAGAAQAARSLARTPAAPAPTPGPATTPAPPAATPAA
                     VTIPDATFDIPYAGPSVRKRARERCIDLREVKGSGPRGRILPADVDAFAAALTPAAKP
                     SVPAAAGTGAGLDLLPWPKVDFAKFGPVETKPLSRIKRISAANLHRNWVMIPHVTSHD
                     EADITELEEFRVRLNKENEKSG"
     gene            complement(1131..3788)
                     /gene="aceE"
                     /locus_tag="VM910_24825"
     CDS             complement(1131..3788)
                     /gene="aceE"
                     /locus_tag="VM910_24825"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HVI65780.1"
                     /translation="MLAMTDADPVETGEWIDSLRAVVHHQGRERASFLLDRLSEEAQL
                     AGASAPLTLNTPYVNTIPPEREERANWDRDIEHRIRSIIRWNAVAIILRANKGSSELG
                     GHIASFQSAATLYDIGFGHFWRAPSDKHGGDLVYIQGHSGPGIYARAFVEGRLTEQQL
                     LNFRQETGGCGLSSYPHPWLMPEFWQFPTVSMGLGPLMAIYQARFLKYLHGRGIADTA
                     ARKVWAFMGDGEMDEPESLGAVSLAGRERLDNLIFVINCNLQRLDGPVRGNGKIVQEL
                     EAVFRGAGWNVIKVLWGSNWDPLLAKDISGMLRQRMEECVDGEYQDFKSKNGAYVRQH
                     FFGKYPETAALVKDWSDDEIWALRRGGSDPVKVYAAYAAAIKYVGQPTVILAKTVKGY
                     GMGEAGEGQNITHQQKKMGLEALRKFRDRFAIPVSDEKIEEIPFLTLPADSSEMKYLR
                     ERRATLGGPLPQRRRTSKALEVPELPAFSAQLASTESREISTTMAFVRILSTLLRDKV
                     IGRFIVPIVPDESRTFGMEGMFRQLGIFSQLGQLYKPQDADQLMFYREDKSGQMLQEG
                     INEAGAMSSWIAAATSYSTSNAPMIPFYIYYSMFGLQRVGDLAWAAGDMRCRGFLLGG
                     TAGRTTLNGEGLQHEDGHSQLLAATIPNCVAYDPTFSYEVAVIIQDGLRRMFRDQEDV
                     YYYITLMNENYSHPAMPAGAEEGIRRGMYLLRDGGNDKGPRVQLLGSGTILREVIAGA
                     DLLAQDFGVSADIWSCPSFNELRRQGLETDRWNLLHPTQAPRPSYVEQCLSPRQGPVV
                     ASTDYMRSFADQIRPFVPRRYVCLGTDGFGRSDYRIALRKFFEVDRHYVATAALNALA
                     QEGAIPKSKVEAAVTKYGIDPERPPPWKM"
BASE COUNT          694 a         1214 c         1185 g          748 t
ORIGIN      
        1 gccggatttc tcgttctcct tgttgagacg cacccggaat tcctcgagct cagtgatgtc
       61 ggcttcatcg tggctggtga catgcgggat catcacccag ttgcgatgca ggttcgcagc
      121 cgagatcctt ttgatgcgcg agagcggctt cgtctcgacc gggccgaact tggcaaagtc
      181 gaccttcggc cacgggagca gatcgagacc agctcctgtt ccggccgctg ccggcacgct
      241 cggcttcgct gccggtgtga gcgctgcggc gaacgcatca acgtcggccg gtagaatgcg
      301 gccgcgcggt ccgctgccct tgacctcgcg cagatcgatg caccgctcgc gcgcgcgctt
      361 gcgcaccgaa ggcccggcat aaggaatgtc aaaagtcgcg tcgggaatgg tgacagcagc
      421 gggcgtcgcc gccggcggcg cgggggtcgt ggctggacct ggcgtcggcg cgggcgcagc
      481 cggagtgcgg gctaaagagc gagcggcctg cgctgcccct gcctcggtga tcaaagtgag
      541 aatcacggtt ccctcgctga cacggtcgcc gaccttgacc ttcaggtcag ccaccttccc
      601 accaagcgga gagggcactt ccattgaggc cttgtcggat tctagcgtca ccagcggctg
      661 ctccgcggcg atggtgtcgc ccggcttgac catgacctcg atgatcggca cgtctttgaa
      721 atcgccgatg gcgggaacgc ggacgtcggc gacgccggct ggcgcgctcg tcggtgccgg
      781 tggagcggac acggcgggct tcaccggttc ctcggcaggg ccagcaccat ccacttcgag
      841 catcaggatc gatgccccct cactaacctt gtcgccgacc ttaacggcga gcgatttgac
      901 cacgcctgcg gccggcgaag gcacttccat cgacgccttg tccgattcca gcgtgattag
      961 gggagcgtcg acgcccaccc gctggccggg ctgcaccgca atctcgatga tgggcacatt
     1021 cttgaaatcg ccgatatcgg gaatgcggat ctctctcagc tcacccatcg cactgccctc
     1081 ccaacatacg caggctgcgc gttacacttg ttggtgctct tgccctcttc tcacatcttc
     1141 cagggtggcg gtcgctctgg atcgatcccg tatttggtga cggcagcctc tactttgctc
     1201 ttgggaatgg caccctcttg cgcgagcgcg ttgagcgcag ccgttgccac gtagtggcgg
     1261 tcaacttcaa aaaacttgcg cagcgcgata cgataatcgc tgcgaccgaa gccatccgtg
     1321 cccagacaca cataacggcg tggcacaaac ggcctgattt gatccgcgaa gctgcgcatg
     1381 taatccgtgg atgccacaac aggtccctgc cgggggctca gacattgctc aacgtagctc
     1441 gggcgtggtg cttgcgtggg gtgaagcaga ttccagcggt cagtttccag gccctgacgg
     1501 cgcagctcgt tgaagctcgg acagctccaa atatccgcgc tcacgccgaa gtcctgcgct
     1561 agcaggtcgg ccccggcgat cacctcgcgc aggatggtgc ccgaaccgag cagttggacg
     1621 cgcgggcctt tgtcattgcc accgtcgcgc aacaggtaca tgccgcgccg aattccctcc
     1681 tccgcgcccg ctggcatcgc agggtgggaa tagttctcgt tcatcagggt gatgtagtag
     1741 tagacatcct cctgatccct gaacatgcgc cgcagtccat cttggatgat gacagcaact
     1801 tcataggaga aggtcggatc gtaggcgacg cagttgggga tggtagcggc gagcagctgg
     1861 ctgtgaccgt cctcgtgctg aagcccttcg ccgttgaggg ttgtcctccc cgcggtaccg
     1921 cccagcagga atccgcggca gcgcatatca ccggcggccc aggcaagatc tcccacgcgc
     1981 tggagcccga acatcgagta atagatgtag aatggaatca tcggcgcgtt gctagtgctg
     2041 taggaggtcg cggcggcgat ccacgaggac atggcacccg cctcgttgat gccctcctgc
     2101 agcatctgcc cggacttgtc ctcgcgataa aacatcagct ggtcggcgtc ctgcggtttg
     2161 tagagctggc cgagctgcga gaagataccg agctgacgaa acattccctc catgccgaag
     2221 gtgcgcgatt catccgggac gatgggcacg ataaaccggc cgatgacttt gtctcgcaac
     2281 aacgtgctga ggatgcgcac gaaggccatc gtggtagaaa tctcgcggct ttcggtgctt
     2341 gcaagctggg cgctgaacgc ggggagctct ggcacctcaa gggcctttga ggtacggcgg
     2401 cgctgcggca gagggcctcc cagcgtggcg cggcgctcgc gcagatattt catctccgag
     2461 ctgtcggccg ggagagttag gaacgggatc tcttcgatct tctcatccga gaccgggatg
     2521 gcgaagcggt cgcggaattt gcgcagggcc tctaacccca tctttttctg ctgatgggtg
     2581 atgttttgtc cttcgccggc ctcgcccatg ccatagccct tcaccgtctt ggcgaggatg
     2641 acggtcggct gcccaacata ctttatggcg gcggcatagg ccgcgtacac cttaaccgga
     2701 tcggagccgc cgcggcgcag cgcccagatc tcgtcgtcgc tccagtcctt caccagcgcg
     2761 gctgtctcgg gatatttgcc gaagaaatgc tgacgcacat aggcgccgtt cttggatttg
     2821 aaatcctggt attcgccatc gacgcactcc tccatgcgct ggcgcagcat tccagagata
     2881 tccttggcca gcagcgggtc ccagttgctc ccccatagca ccttgatcac gttccatccg
     2941 gcgccgcgga agacagcctc gagctcctgc acgatcttgc cgttgccgcg cacgggcccg
     3001 tcgaggcgct gcaaattgca gttgataaca aagatgagat tgtcgagccg ctcccgcccg
     3061 gcaagcgaca cggctccgag cgattctggc tcgtccattt ctccgtcgcc catgaaagcc
     3121 cacaccttgc gcgctgcggt atcggcgatg ccgcggccgt gcagatattt gaggaagcgc
     3181 gcctgataga tcgccatcag cgggccgagc cccatagata cggtggggaa ctgccaaaat
     3241 tccggcatca gccagggatg aggataggac gagaggccgc agcctcccgt ctcctgacgg
     3301 aaattcagaa gctgttgctc agtgagccgg ccctccacga aggcccgcgc gtagatgcca
     3361 ggcccggagt ggccctggat atagacgagg tcgccgccgt gcttatccga cggcgcgcgc
     3421 cagaagtgac caaagccgat gtcatagagc gtggccgccg actggaagct ggcaatatgg
     3481 ccgccgagtt cggaggaccc cttgttggcg cgcagaatga tcgcaaccgc gttccagcga
     3541 atgatcgagc ggattcgatg ctcgatatcg cggtcccagt tcgcacgctc ctcgcgctcc
     3601 ggaggaatgg tgttgacata tggggtattg agtgtgagcg gcgcgctcgc accggcgagc
     3661 tgcgcctcct cactcaatcg gtcaagcaaa aacgacgccc gctctcggcc ttggtgatgc
     3721 acgacggcgc gcagcgaatc gatccattcg cccgtctcca cgggatcggc atccgtcatc
     3781 gccagcatga gctcctccct gactttgtca gactatttac ggtttggtcg caacctcccc
     3841 g
//
LOCUS       DATLJT010001077         1509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1453971,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001077 DATLJT010000000
VERSION     DATLJT010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453971"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="ptsN"
                     /locus_tag="VM910_24830"
     CDS             complement(<1..455)
                     /gene="ptsN"
                     /locus_tag="VM910_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HVI65781.1"
                     /translation="MEIADLITPRSVVAQLRATNKKQALQELAKRAAALTGIQERTIY
                     DVLIERERLGSTGIGMGIGIPHGRLSGLETLCGIFARLDRPVPFDAIDDQPVDLIFLL
                     LAPEGAGADHLKALARVSRLLRDRNVCEKLRGTDNADALYALLIDRTPS"
     gene            complement(470..1057)
                     /gene="raiA"
                     /locus_tag="VM910_24835"
     CDS             complement(470..1057)
                     /gene="raiA"
                     /locus_tag="VM910_24835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVI65782.1"
                     /translation="MQLTVTGKQIDTGTVLRQHVEASLGAILGKYFKTAIEAHVVFSK
                     EAHLNRAEVSIHIGRRIVANAGASATETYAAFDAAAERIAKQMRRYKRRLRDHHAEAR
                     EAFEVGERARDYVLAPLAEEGDEGPAVVAEMSTDVPNLTVSEAVMRMDLANAPVLLFR
                     NRSHGELNIVYRRLDGNIGWIDPELDPTRLRHGEE"
     gene            complement(1208..>1509)
                     /locus_tag="VM910_24840"
     CDS             complement(1208..>1509)
                     /locus_tag="VM910_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HVI65783.1"
                     /translation="VTSNKYMATPRGLYELKYFFTSAISASGGSGAHSAEAVRHRIRA
                     LIDGEPPEGTLSDERIVELLHHEGVDIARRTVAKYREAMRIPSSVRRRRDKLLAL"
BASE COUNT          286 a          465 c          450 g          308 t
ORIGIN      
        1 tggctgggag tacgatcgat caagagggcg tagagtgcat ccgcgttgtc cgtgccgcgg
       61 agcttttcgc atacgttgcg atcccgcaat agtcgggaca cccgcgctag cgctttgaga
      121 tggtccgctc ctgcgccttc cggcgctaac agcagaaaaa tcaagtcgac gggctgatcg
      181 tcgattgcgt caaacggaac gggccggtcc aaccgtgcga aaattccgca cagcgtctcg
      241 agaccagaaa gtctgccatg cgggataccg atacccattc cgatcccggt tgagccaagc
      301 cgctcgcgtt cgattaggac atcatagatg gtgcgctctt ggatcccggt aagcgccgcg
      361 gctcgcttag caagctcctg taaagcctgc tttttattgg tcgcacgcag ctgagcgacg
      421 acactgcgcg gcgttatcag atcggcaatc tccatcgcca atcgcgaggt cactcctcgc
      481 cgtgacgtag ccgggtgggg tcgagttccg gatcgatcca cccaatattg ccatccaggc
      541 gacgatagac aatgttaagc tcgccgtgcg agcggttgcg gaacagcaac accggcgcgt
      601 tggcaagatc catccgcatc accgcctcgc tgacggtcag gttgggcacg tcggtgctca
      661 tctcggcaac gaccgctggc ccttcgtctc cttcctcggc aaggggcgct aacacgtaat
      721 ccctggcacg ctcgccaacc tcaaaagcct cgcgcgcctc cgcatgatgg tcgcgcaagc
      781 gacgtttgta tcggcgcatc tgcttggcga ttcgctctgc ggctgcatca aaagccgcgt
      841 aggtctccgt ggccgaggcg ccggcatttg caacgattcg ccggccgata tggatggaaa
      901 cctcggcccg gttcagatgc gcctctttag agaacaccac gtgggcttcg atggcggtct
      961 tgaagtattt cccgaggatc gcgccgagac tcgcttcgac atgctggcgc aacacagtgc
     1021 cggtgtcgat ctgcttgccc gtgacggtca gttgcatgga tgcgtttacc tcttcccgcc
     1081 gaagctagga tgagatcgag gcctcgggga tcgaaggtgg gaacccgccg gcaagcgatc
     1141 aagcggcata cggtagggat cccttgtttt tgcgtcaagc agcgccgcta ctgtgtcgtc
     1201 tcccccttca aagcgcgagc agcttgtccc ggcgccgccg aaccgaagaa gggatgcgca
     1261 ttgcctctcg gtacttagct accgtacggc gggcaatgtc caccccctcg tggtgtagca
     1321 gctcgacgat gcgctcgtcc gagagtgtcc cttccggggg ttcgccgtcg atcaaggcgc
     1381 ggatgcgatg gcgcaccgct tccgccgaat gcgcgccgct cccgcctgaa gccgagatcg
     1441 ccgaagtaaa gaaatatttg agctcataaa gaccacgcgg tgtggccatg tatttgttgc
     1501 tggtcactc
//
LOCUS       DATLJT010001078         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4210733,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001078 DATLJT010000000
VERSION     DATLJT010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4210733"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VM910_24845"
     CDS             complement(<1..769)
                     /locus_tag="VM910_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI65784.1"
                     /translation="MRRAIRFALVAAATMAITPAAAQPKERVVNIYNWSDYMAAGVVE
                     DFSKETGIKVRYDTFDSNDTLETKLLAGKSGYDVVVPTAYFLERQIKAGVFQKLDKAK
                     LPNLANMWPEISQRLAAYDPGNQYAVNYMWGTTGIGYNVKKARELLGASGKIDSWDIV
                     FKPENVAKFKDCGVHLLDSSDDILAASLHYLGLDPNTTNEADLQKAADLVSKIRPYVR
                     KFHSSEYLNALASGEICLVVGFSGDVKQSQKRAAEAKN"
     gene            complement(864..1199)
                     /locus_tag="VM910_24850"
     CDS             complement(864..1199)
                     /locus_tag="VM910_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI65785.1"
                     /translation="MSAQLGRENVHDLAPQEVARGIAEGKVLLVDVREPNETEVERYP
                     EAFYLPMSQFDPAAIPDPQGRRVVFACRSGNRSVTASLMAQAAGLPYDAHLAGGIKAW
                     KELGLPTES"
     gene            complement(1373..>2204)
                     /locus_tag="VM910_24855"
     CDS             complement(1373..>2204)
                     /locus_tag="VM910_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HVI65786.1"
                     /translation="LVGVLLLVALFRSSSALASAYGIAVTGTMVATVTMAFVVVWKVW
                     NWPPLAAAALIAPFLFIDLTFLGANLLKVVEGGWVPLALGGLVMVVMYTWRRGSRLLF
                     EKTRRQEISLHDLVRMLEKKPPIRVPGTAVFLTSTPESAPTALMHSLKHYKVLHEHNV
                     ILTIETADLPRAEPAERVRIEPVGKTFSRVVLRFGFMETPNVPRALAIARKHGWQFDI
                     MSTSFFLSRRALKPAAHSGMPRWQDRLFIMLTRIANDATDYFQIPTGRVVEVGTQVTI
                     "
BASE COUNT          414 a          689 c          708 g          393 t
ORIGIN      
        1 cgttcttggc ttccgcggcg cgcttttgcg actgcttgac gtcgccggaa aagcccacca
       61 cgagacagat ctcgccggag gcgagcgcgt tgaggtactc cgacgagtgg aacttgcgca
      121 cgtagggcct gatcttgctc acgagatcgg cggccttttg cagatcggcc tcgttggtgg
      181 tgttggggtc gagaccaaga tagtgcagcg acgccgccag gatgtcgtcg gaggaatcga
      241 gcaaatgcac gccacagtcc ttgaacttgg ccacgttctc gggcttgaag acgatgtccc
      301 agctgtcgat cttgccgctc gcccccaaca gctcacgcgc ctttttgacg ttgtagccga
      361 tgccggtcgt tccccacatg taattgacgg cgtactggtt gccgggatcg tacgccgcca
      421 atcgctgtga gatctccggc cacatgttgg cgaggttcgg cagcttcgcc ttgtccagct
      481 tctggaaaac gccggccttg atctggcgtt cgagaaaata ggccgtcggc accaccacgt
      541 cgtaaccgga tttgccggcc agaagcttcg tctcgagcgt gtcgttggaa tcgaaggtgt
      601 cgtagcggac cttgatcccg gtctccttgc tgaaatcctc gacgacgccc gcagccatgt
      661 agtcggacca attgtagatg ttgacgacgc gctctttcgg ctgcgcggca gcaggcgtga
      721 tcgccatggt ggcggctgca acgagggcaa accggatcgc tcggcgcatg gatgggctcc
      781 tgcaagacca tcggatgaaa aaggtcgggg aaacgcgtgc tcgggaagat ggcgaaaggg
      841 gttgcgcgtc gccgcagcgc gcttcaactc tcggtcggca aaccgagttc cttccacgct
      901 tttatccccc cggcgagatg ggcatcgtag ggcagccccg cggcttgcgc catcagcgac
      961 gcggtgaccg accggttgcc cgagcggcag gcgaacacga cccggcggcc ctggggatcg
     1021 ggaatcgcgg ccggatcgaa ctgcgacatc ggaagataga acgcctccgg atagcgctcg
     1081 acttcggtct cattgggctc gcggacatcg accagcagaa ccttgccctc ggcgatcccc
     1141 cgcgccacct cttgcggggc taaatcgtgc acgttctctc ggccaagctg agcggacatg
     1201 aggtctactc ctcaattcgg ggcgcggcgt cgccgcccat gaaaacaagt cgcgcgtcac
     1261 actgcaagaa tcaagacccc ggcgattgcg gtgccggcta gtgtggcgat tccgaagtcg
     1321 catcattgtg ccgcagcctc gtttgcgaac ttggaatcga aggacactag cgtcagatcg
     1381 tcacctgcgt gcccacttcc accacccgac cggtcgggat ctgaaaatag tcggtcgcgt
     1441 cgttggcgat gcgcgtgagc atgatgaaca agcgatcctg ccagcgcggc atgccggaat
     1501 gggcggccgg cttgagcgcg cgcctcgaca ggaagaacga ggtcgacatg atgtcgaact
     1561 gccagccgtg cttacgggcg attgcgagcg ctctggggac gttcggcgtt tccatgaacc
     1621 cgaagcgcag gaccacgcgc gagaacgtct tgcccacggg ctcgatgcgc acgcgctcgg
     1681 ccggctcggc tcgcggcagg tccgccgtct cgatggtcaa aatgacgttg tgctcgtgca
     1741 gcaccttgta atgcttgagg ctgtgcatga gcgcggtcgg cgcggactcg ggcgtgctgg
     1801 tgagaaacac ggccgtgccc ggcacccgga tcggcggctt tttttcgagc atgcgcacga
     1861 gatcgtggag cgagatttcc tggcgccggg tcttctcgaa cagcaggcgg ctcccgcgcc
     1921 gccacgtgta catgacgacc atcaccaggc cgccgagcgc cagcggcacc cagccgccct
     1981 cgaccacctt gagcagattg gcgccaagaa aggtcaggtc gatgaagaga aagggcgcga
     2041 tcaacgccgc cgccgccaac ggcggccagt tccacacctt ccagacgacg acgaaggcca
     2101 tggtgaccgt ggcgaccatg gtgccggtca ccgcgatgcc gtaggccgac gcgagcgcgc
     2161 tcgaagagcg gaacaacgcc acgagcagca agaccccgac cagc
//
LOCUS       DATLJT010001079         5886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2372351,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001079 DATLJT010000000
VERSION     DATLJT010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372351"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1664
                     /locus_tag="VM910_24860"
     CDS             414..1664
                     /locus_tag="VM910_24860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HVI65787.1"
                     /translation="MDRLLRRLLTKFVRRGTMLFTTADGGTFTCGDGTGEPVHARFLT
                     AEAERRVVLNPELALGEIYMEGQFVIEKGTIADALAILMNQPDSVPHWAKPRWWMRYL
                     LRRLRQFNGRGRSRNNVAHHYDLDGRLYSLFLDADKQYSCAYFETPDTNLDDAQLAKK
                     RHLAAKLLIRRGHRVLDIGSGWGGLGLYLAEMTGANVTGITLSTEQLQASNARAAEKN
                     LTSSAKFLLQDYRDIEGPFDRIVSVGMFEHVGVDFYDSFFKRCAELLTDNGIMMLHSI
                     GRSTGPDVTSPWITKYIFPGGYVPALSEVFPSIERAGLLVCDTEILRLHYAETLKAWR
                     ERFMARREEAVQLYDERFARMWEFYLAASEMAFRKQNMMNFQVQLTKRQGVVPITRDY
                     VVREEARLRHLERGSRPRLRIAGE"
     gene            2038..2160
                     /locus_tag="VM910_24865"
     CDS             2038..2160
                     /locus_tag="VM910_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65788.1"
                     /translation="MAQKHRERSFEVLGDLRTDYALIAVGAGAALIALLYLVLT"
     gene            2443..3945
                     /locus_tag="VM910_24870"
     CDS             2443..3945
                     /locus_tag="VM910_24870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVI65789.1"
                     /translation="MASPDSNVLKLASDPATPTYRSAPHNIEAEQSLLGAILVNNDAF
                     YRVSDFLEPKHFFETLHQKIYETAGSLIRMGKVATPVTLKTFFPADADILGMTVSQYL
                     ARLAAEATTIINAQDYGRTIYDLSLRRDLIGIGEDMVNVAFDAPVDFAPRAQIEDAER
                     KLYELAESGRYDGGFQKFSQALTVAVDMAAKAFQRDGKLSGIATGLRDLDIKMGGLQQ
                     SDLIIVAGRPGMGKTALATNIAYNVARAYRGEVQADGTTRAVNGGIVGFFSCEMSAEQ
                     LATRIIAERTGIPSSTIRRGGISEGDFEKIRDYSIELQSLPFYVDETGGLSIAQLSAR
                     ARRLKRQKGLDLIVVDYIQLLQGSSRRSSESRVQEVTEITTNLKALAKELNVPVIALS
                     QLSRQVENRDDKRPQLADLRESGSIEQDADVVLFVFREEYYLANKEPRAGTPEYEKWQ
                     LDMSLVHGKGEVIIGKQRHGPTGTVELSFDAAVTRFGDLAHDAQLPDRTY"
     gene            4037..5272
                     /gene="alr"
                     /locus_tag="VM910_24875"
     CDS             4037..5272
                     /gene="alr"
                     /locus_tag="VM910_24875"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVI65790.1"
                     /translation="MNIASDPKTSLAGSTLSPEANPALATSTGVLTVNLDAIVANWKK
                     LEKTAVPAECAAVVKANAYGLGAEQVARALASAGCKTFFVATLDEARSVRPAVPSATL
                     YVLDGVFQNTADAYARIDCKPVIGDLNELAEWDVFCRRTGWSGGAAIHIDTGMNRLGL
                     TIAEAQGLIPRINAGNHGITLVMSHLVSAELINNTTNARQLATFREIASEFSGVPASL
                     SNSSGIYLGPQFVFDLVRPGAALYGVNPTPEADNPMQPVAELKARIVQIRNVERGETV
                     GYRGAWTARRPTRLAIVAAGYADGYFRAASASDGTRGGEVVVAGKRCPVAGRVSMDLI
                     AIDITDLPANAARRGHMVTLLGEGITVDELARHFGTIGYEVLTSIGARYTRIYKGGAA
                     QGAGRDSAQAESAASTSAA"
     gene            5269..>5886
                     /locus_tag="VM910_24880"
     CDS             5269..>5886
                     /locus_tag="VM910_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HVI65791.1"
                     /translation="MKIATFNINNINRRLDNLLAWLRSARPDIACLQELKASDNEFPL
                     RAIEKAGYRAVFRGQKTWNGVAILSRKSDPVLTRIELPGDDGDRESRYIEAAVSGVIV
                     ACIYAPNGNPQPGPRFDYKLDWLRRLRLHAARLLRQDLPVVLAGDYNVAPTGLDIYPT
                     RSWDKDALIQPKSRAAFAALVAQGWTDAIRTLHPSKPMFTFWHYKR"
BASE COUNT         1167 a         1897 c         1765 g         1057 t
ORIGIN      
        1 aaatctggga cggggacttg ccggcatcga ggttctcctg cgccagcgtc cgctccgccg
       61 ccaaacagcc cactagaagc atcaccgtcg ctagactgag cgctcggctc aacattctcg
      121 acttctccag atgcggaaag cccgtccagc gcagccagga ctcgcttccg gcgccgggcg
      181 gggtcacctt ttagccgatt atcgcgccaa tggaagcgcg catagccccg ccaactgcgt
      241 gatgggcata atttggaata tccgctttgt ctacaacaca ctagcggaca tccaccggac
      301 ttattatgat tatattcagc cgcatttgga accaatgggt tccaaatgcg tcgaacggga
      361 cggccgggaa aattagtgcc tgccctcgcg ggatcgctcg ttgggtgatt tcgatggacc
      421 gtttgttgcg tcgcttgctc acgaagttcg ttcgccgcgg tacgatgctg ttcacaaccg
      481 cggacggggg gacgttcacc tgcggcgatg gaaccggcga accggttcac gcacgctttc
      541 tcacagccga agctgagcgt cgggttgtct tgaaccccga acttgcgctg ggcgaaatct
      601 acatggaagg acaattcgtc atcgagaaag gtaccatcgc ggacgcgctg gccattctga
      661 tgaaccagcc cgacagcgtg ccgcactggg ccaagccgcg atggtggatg cgctatttgc
      721 tgaggcgcct caggcaattc aacgggcgtg gccggtcgcg caacaacgtc gcgcaccatt
      781 acgatctcga cggacggctc tactcgctgt tcctggatgc cgacaagcaa tatagctgcg
      841 cctatttcga gacgccggac acaaacctcg acgacgcgca actcgccaaa aaacgtcatt
      901 tggcggcaaa gctcttgatc cggcgcggcc accgcgtact cgacatcgga tccggttggg
      961 gcgggcttgg gctctatctc gccgagatga cgggcgccaa cgtcaccggc atcacactat
     1021 ccaccgagca attgcaggcg tcgaatgcgc gcgcggcgga gaagaacctg accagctcgg
     1081 cgaaattcct gctgcaggat tatcgtgaca tcgagggacc atttgaccgc atcgtgtcgg
     1141 tgggcatgtt cgagcatgtc ggggtcgatt tctacgacag cttcttcaag cgatgcgcgg
     1201 aacttttgac cgacaacggc atcatgatgc tgcattcgat cggccgctcg accggtcccg
     1261 acgtcaccag tccctggatc acgaaatata tcttccccgg cggttacgtt ccggcgttgt
     1321 cggaggtttt tccctcgatc gaacgcgccg ggcttctggt ttgcgacacc gaaatcctgc
     1381 gcctgcatta tgcggaaacg ctcaaggcgt ggcgcgagcg cttcatggcg cggcgcgagg
     1441 aagccgtcca actctatgac gagcgctttg cgcggatgtg ggagttctat cttgcggcgt
     1501 cggaaatggc gttccgtaag caaaacatga tgaatttcca ggttcagctc acaaagcgcc
     1561 agggcgtggt gccgatcacg cgcgactatg tcgtgcgcga ggaagcacga ttgcgccacc
     1621 tcgaacgggg cagccggcca cggctgcgga tcgcaggcga ataagacaaa accggatcag
     1681 ttgcgaagac tgaaggtgga ggcgccgggc gggtgggccc gaggcgggcg tgcaagctgc
     1741 gcccgtaccg ccgccagcac cagctggtcg cacggcagtt ttcggacctc aggccgagca
     1801 atctgctcca tggtcgcaat caggcgcaac agccattggc ggtcgccgtt tagcgccgcc
     1861 cttttcgatt gccatgtctg cacttgccgg ggcatcgccg ctctttgcct caagcttgac
     1921 ccaaaccgca ccacaccaac tttggaacca accgcgcgtt cccggccgga aagaaaaccg
     1981 cggcgattgt gaaccggctc acacaacgtc gctttgccct gtcgtaaagc tccggtcatg
     2041 gcccagaagc accgcgagcg atctttcgaa gtcttgggcg atctgcgcac cgactacgca
     2101 ctcatcgcag tcggcgccgg agcggccctg atcgcgctgc tttacctggt tctgacctga
     2161 cggcctgtcg caggacaatt tctcgctctg aataggtctg gccgagagcg ataaggttca
     2221 ccggccgtcg aactttcctc aggaaatccc ctaaaatttt tgggtaattg cgcgaaatcg
     2281 gccaaaaaac cgtcaagcgg catgctgcgg gcgctccgga ttcacacctg gcgttgtgtg
     2341 aatcgggcat aagaacgcat gacactttcg cggcggcctg gagtagcgct tgatcgccgc
     2401 cctcatccca ataacgacca aatccgaaga acaattctgc ccatggcttc ccctgattcg
     2461 aacgtcctca agctcgcctc cgacccggca actccgacct atcggagcgc accgcacaat
     2521 atcgaggccg agcagagcct tttgggggcc attctcgtca acaacgacgc cttctaccgg
     2581 gtctcggatt ttctggagcc gaagcacttt ttcgagacgc tgcaccagaa gatctatgag
     2641 accgcgggta gcctgatccg gatgggcaag gtcgcaaccc ccgtcacgct caaaaccttc
     2701 tttcccgcgg acgccgacat cctgggcatg acggtgagcc aatacctggc acgcctcgcg
     2761 gcagaagcga ccaccatcat caatgcccag gactacggac gcacgatcta tgacctgtcg
     2821 ctgcgccgcg acctgatcgg aatcggcgag gacatggtga acgtggcctt tgacgcgccg
     2881 gtcgattttg cgccgcgcgc tcagatcgag gacgctgagc gcaagctcta tgagctggcc
     2941 gaatccggcc gctacgacgg cggcttccag aaattctcgc aagcgctcac cgtcgccgtc
     3001 gacatggcgg caaaggcctt ccagcgcgac ggaaaactgt cgggcatcgc caccggcctg
     3061 cgcgatctcg atatcaagat gggcgggctg cagcaaagcg acctcatcat cgtcgccggc
     3121 cgacccggca tgggcaagac ggcgcttgcc accaacatcg cctacaacgt ggcgagggcc
     3181 tatcgcggcg aggtgcaggc ggacggcacg acccgagccg tcaatggcgg catcgtcggc
     3241 ttcttctcct gcgaaatgtc ggccgagcag ctcgccaccc gtatcatcgc cgagcgcacc
     3301 ggcattccct cgagcacgat ccgccgcggc ggcatcagcg agggcgattt cgagaagatc
     3361 cgcgactatt cgatcgagct gcagtcgctg ccgttttatg tcgacgagac cggcggcctg
     3421 tcgatcgcgc agctctcggc gcgcgcacgc cgcctgaaac gacaaaaggg actggacttg
     3481 atcgtggtcg actatatcca gcttctgcag ggcagtagcc gtcgctcctc agaaagccgc
     3541 gttcaggagg tcacggagat caccaccaac ctgaaggcgc tggcaaagga gctcaacgtt
     3601 cccgtaatcg cactatcgca gttatcgcgc caggtcgaaa accgcgacga caagcgtccg
     3661 caacttgccg acttgcgcga atccggttcg atcgagcagg acgccgacgt cgtgctgttc
     3721 gtgttccgcg aagaatatta tctcgccaac aaagagcccc gcgccggcac gcccgagtat
     3781 gaaaagtggc agctcgacat gtcgctggtg catggcaagg gcgaggtcat catcggaaag
     3841 cagcgccacg gcccgaccgg cacggtcgag ctgagcttcg acgcggcggt gacgcggttt
     3901 ggcgacctcg ctcatgacgc acaattgccg gatcggacgt attagcgcgc cggtattcac
     3961 ccgcgctgaa cgcgcccgtc cggatcggtg gaaccagagt tgaacaggtc ccgtcccgcg
     4021 cgtaaaactc gtctccatga acatcgcctc cgatccaaag acctcattgg ccggctcaac
     4081 gctgtcgccc gaagcaaacc ctgcgctggc gacgtcgacc ggcgtgttga ccgtcaacct
     4141 cgacgccatc gtcgcgaact ggaaaaagct cgagaagacc gccgtgcccg cagaatgcgc
     4201 cgccgtagtc aaggcgaacg cctatggact tggcgccgaa caggtcgcgc gcgcgctcgc
     4261 ctccgccggc tgcaagacat tcttcgtcgc aaccctcgat gaagcccgta gcgtgcgacc
     4321 ggcggtgcct tcagccacgc tctatgtgct cgacggtgtt ttccagaaca ccgccgatgc
     4381 ctatgccagg atcgactgca agcccgtgat cggcgacctc aacgagctcg ccgagtggga
     4441 tgtgttttgc cgccgcacag gctggagcgg cggcgcggcg atccatatcg acaccggcat
     4501 gaaccggctc ggacttacga ttgccgaggc gcagggcctc attccccgca tcaatgccgg
     4561 caatcacggc atcacgctgg tcatgagcca cctcgtctcc gccgaactga tcaacaatac
     4621 caccaacgcc aggcagcttg cgacattccg cgagatcgcg agtgagttct ccggcgtgcc
     4681 ggcgtcgctg tcgaattcct ccggcatcta tctgggccca cagttcgtgt tcgacctggt
     4741 gcgtccgggg gcggcactct acggcgtcaa cccgacgccg gaggccgaca atccgatgca
     4801 gccggttgcg gaattgaagg cgcgcatcgt gcagatccgc aatgttgagc gcggcgaaac
     4861 cgtcggctat cgcggcgcct ggaccgcacg gcggccaacc aggctggcga tcgtcgcggc
     4921 gggctatgcc gacggctact tccgcgcggc aagcgcgagc gacggcaccc gcggcggcga
     4981 ggtcgtcgtt gcgggcaagc gctgtccggt cgcaggacgc gtctcgatgg acctgatcgc
     5041 aatcgacatc accgacctgc ccgccaacgc cgcgcgccgc ggccacatgg tgacgctgct
     5101 cggcgaaggc atcaccgttg acgagctcgc ccgtcatttc ggcacgatcg gttatgaagt
     5161 gctgaccagc atcggcgcgc gctacacgcg gatctacaag ggcggcgcgg ctcaaggtgc
     5221 tgggcgggac agcgcgcagg ccgagagcgc cgcctcgact tccgctgcgt gaagatcgcg
     5281 accttcaaca tcaacaacat caaccggcgg ctggacaacc tgctcgcctg gttgcgcagc
     5341 gccaggcccg acatcgcctg cttgcaggag ttgaaggcct cggacaacga gtttccgttg
     5401 cgcgccatcg aaaaggccgg ctatcgcgcg gtgttccgcg gacaaaagac gtggaacggc
     5461 gtcgcgatcc tctcgcgcaa atcggatccg gtgctgacga ggatcgaact gccgggcgat
     5521 gacggcgatc gcgagtctcg ctacatcgaa gccgcggtct ccggcgtcat cgtcgcctgc
     5581 atttatgcgc cgaacggcaa tccgcagccc ggcccgagat tcgactacaa gctcgactgg
     5641 ctgcgacggc tgcgcctgca cgccgcaagg ctcctgaggc aggatttgcc ggtcgttttg
     5701 gccggcgatt acaacgtcgc gccaaccggg ctcgacatct atcccacgcg ctcatgggac
     5761 aaggacgcgc tgatccagcc gaagagccgt gcggcttttg ctgcgctcgt ggcgcagggc
     5821 tggacggacg cgatccggac cttgcatccg tcaaaaccga tgttcacctt ctggcactac
     5881 aagcgc
//
LOCUS       DATLJT010001080         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4441402,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001080 DATLJT010000000
VERSION     DATLJT010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4441402"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /gene="grpE"
                     /locus_tag="VM910_24885"
     CDS             complement(<1..473)
                     /gene="grpE"
                     /locus_tag="VM910_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVI65792.1"
                     /translation="MSDPDLQKKDPVNRGQQTGEPVASKPYVMPDDPEEGSVEALKRE
                     AAESRDKMLRTLAEMENLRKRTSREVTDARTYGITGFARDVLEIADNLQRALDAVPAE
                     AKATADPGLKGLIEGVELTERALLNALEKNGVTKFDPTGERFDPNFQQAMYEVPDP"
     gene            complement(669..1757)
                     /gene="hrcA"
                     /locus_tag="VM910_24890"
     CDS             complement(669..1757)
                     /gene="hrcA"
                     /locus_tag="VM910_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVI65793.1"
                     /translation="MAHHDPIGLIAPAAGLALLNERSRDIFRQIVESYLATGEPVGSR
                     NISRLIAVPLSPASVRNVMADLEQLGLIYAPHTSAGRLPTERGLRFFVDALMQMGDLT
                     DAERQSIQSQLVSVGRAQSVEAALGEALTLLSGLTRAAAVVLTAKSNARLKHIEFVRL
                     EPERALVVLVGEDGQVENRVLTLPPGVPPSALTEASNFLNARIRGRTLAEARLELETA
                     MAQDRAELDQLTQKVVAAGLASWSGGEHEERQLIVRGHANLLEDLHALDDLERVRLLF
                     DDLETKRGVIDLLGRAESAEGVRIFIGSENKLFSLSGSSTIVAPYRDAAGHIVGVLGV
                     IGPTRLNYARVIPTVDYAARVVSRMLGG"
     gene            1925..2638
                     /gene="rph"
                     /locus_tag="VM910_24895"
     CDS             1925..2638
                     /gene="rph"
                     /locus_tag="VM910_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HVI65794.1"
                     /translation="MRPSRRAPDELRAVSLERGVVKYAEGSCLVKFGDTHVLVTATLE
                     ERLPPWLKGQGRGWVTAEYGMLPRATLERTRREASAGKQNGRTVEIQRLIGRSLRATV
                     DLEALGERQITVDCDVLQADGGTRTASITGAWVALADCIGWMKARNMVKSDVLRDNVA
                     AISCGIYNGTPVLDLDYAEDSEAETDANFVMTGDGRIIEVQGTAEKTPFSQEQFLTLM
                     ALARKGVARLVDLQKLAVA"
     gene            2643..3278
                     /gene="rdgB"
                     /locus_tag="VM910_24900"
     CDS             2643..3278
                     /gene="rdgB"
                     /locus_tag="VM910_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HVI65795.1"
                     /translation="MHRHIKGKLVIATHNPGKLAEMRELLAPHGVEAVSAGELGLSEP
                     HETGDTFQANARIKAVAAAKAAQLPAFADDSGLVVDALDGAPGILSARWAGPNKDFDA
                     AMAQIERLLRERGATTQGKRGAHFVSALCVAWPDDHLEEVEARADGTLVWPPRGTAGF
                     GYDPMFMPEGFDRTFGEMTSIEKHGLPPLGLGLSHRARAFLKLAEICLDPR"
     gene            3265..>3437
                     /locus_tag="VM910_24905"
     CDS             3265..>3437
                     /locus_tag="VM910_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HVI65796.1"
                     /translation="MTRAETAAFGVYVHWPFCLSKCPYCDFNSHVRHAAIDEDRFARA
                     FAREIETTAARVPG"
BASE COUNT          615 a         1134 c         1069 g          619 t
ORIGIN      
        1 ggatcgggaa cctcgtacat cgcctgttgg aaattcgggt cgaatctctc tcccgtcgga
       61 tcgaacttcg tcacgccgtt tttctcgagc gcgttgagta gcgcgcgttc ggtgagctca
      121 acgccttcga tcagaccctt caggccggga tcggcggttg ccttggcctc cgcgggaacc
      181 gcatcgaggg cgcgctgcag attgtcggca atttcaagca cgtcccgcgc aaagccggtg
      241 atgccgtaag tccgtgcatc cgtcacctca cggctggtcc gcttgcgcag attttccatc
      301 tccgccagcg tgcgcaacat cttgtcgcgg gattcggcgg cttctctctt gagcgcctca
      361 accgacccct cctcgggatc gtcaggcatg acatagggct ttgacgccac gggctcaccg
      421 gtctgctgcc cccggttcac cggatctttc ttctgcaggt cgggatcact catgagacgg
      481 ccttctcaaa aaaatcgact gggatgaaag ggaggctgtg gctgtgttgg ccgccatatc
      541 gtgcttcgga cggcgaaaat caagcgcccg tgatcgcgta ccctttccaa agttcgcgtt
      601 ccgtttttcg attggaagcc ccagggtcgg actttccgcc ttgcactgca gggatttaaa
      661 gcgccaggtc agccgcccag catccggcta accacgcgag cggcataatc gaccgtggga
      721 atcacccttg cataattaag ccgtgtcggc ccgatcacgc ccaggacgcc gacgatatgg
      781 ccggctgcgt cgcggtacgg cgccacaatc gtcgaggagc ccgatagcga aaacagcttg
      841 ttttcggaac cgatgaagat tcgcacgcct tcggcgctct ccgcgcggcc caacaggtcg
      901 atgacgcctc gcttggtttc gagatcgtcg aacaacagcc gaacccgttc cagatcgtca
      961 agcgcgtgca gatcctccag cagattggcg tggccgcgca cgatcaattg gcgctcctca
     1021 tgctcgccgc cggaccagct cgcaaggccg gccgccacga ccttttgcgt gagctgatcg
     1081 agctcggcac gatcctgcgc catcgcggtt tcaagctcga gccgcgcttc cgccagcgtg
     1141 cggccgcgga tgcgcgcatt gaggaaattc gacgcctccg tcaacgcgga aggcggaacg
     1201 ccgggcggca gcgtcagcac gcggttctcg acctggccat cctcgccgac cagcacgacg
     1261 agcgcgcgtt ccggctcgag ccggacgaat tcgatatgct tcagccgcgc attcgatttg
     1321 gcagtcagga cgacggcggc cgcgcgcgtc agacccgaca atagcgtcaa ggcttcgccc
     1381 agcgccgcct cgacggattg tgcccgtccg accgagacaa gctggctctg gatcgactgc
     1441 cgctcggcgt cggtgagatc gcccatctgc atcagcgcat cgacgaaaaa tcgcaagccg
     1501 cgctcggtcg gcagccggcc ggcggaggtg tgaggcgcat agatcagacc gagctgctcg
     1561 agatcggcca tgacgttgcg caccgaggca ggcgatagcg ggacggcgat caggcgggag
     1621 atgttgcggg agccaaccgg ctcccccgtc gccaggtagc tttccacgat ctgacggaaa
     1681 atgtcgcgcg aacgctcgtt aagcagggca agcccggctg ccggggcgat caaaccgatc
     1741 ggatcgtggt gggccacact taactccctt ccaacctccc attacattta tatttgtccg
     1801 tccaccggct ccctgacaag cgggctttaa cggtcttaaa agcacggtta agggccttgc
     1861 cgccctcctc cgcccctcct acaagctgcg ccagccacat tttgctcgat ccctggagga
     1921 ccccatgcgg ccaagtcgcc gtgccccgga cgaacttcgc gccgtttcgc tcgagcgcgg
     1981 tgtcgtcaaa tatgcggaag ggtcttgcct cgtgaagttt ggcgacaccc atgtgctcgt
     2041 caccgcgacc ctggaggagc ggttgccgcc atggttgaag ggccagggcc gaggctgggt
     2101 cacggccgaa tatggcatgc tgccgcgcgc aacattggaa cggacgcggc gcgaggcatc
     2161 ggccggtaaa cagaacgggc gcacagtcga gatccagcgc ctgatcggcc gctcgttgcg
     2221 cgccaccgtc gatctcgagg cgctcggcga gcgccagatc accgtcgatt gcgacgtgct
     2281 gcaggccgac ggtggcacgc gcaccgcctc gatcaccggc gcctgggtcg cgctcgccga
     2341 ctgcatcggc tggatgaagg cgcgcaacat ggtcaagtcg gacgtgctgc gcgacaacgt
     2401 cgctgccatt tcctgcggca tctacaacgg aacgccggtc ctcgatctcg attatgccga
     2461 ggactccgaa gccgagaccg acgccaactt tgtcatgacg ggggatggtc gcatcatcga
     2521 ggtgcagggc accgcggaaa agacgccgtt ctcgcaagaa cagttcctga ccctgatggc
     2581 gctggcgcgc aaaggcgtgg cgcggctggt ggatctgcag aaactcgcgg tcgcgtgagg
     2641 ccatgcatcg ccacatcaaa ggcaagctcg tgatcgcgac ccacaatccc ggcaagctcg
     2701 ccgagatgcg cgaactcctg gccccgcacg gtgtcgaagc cgtctcggca ggcgagctcg
     2761 gcctctccga gccgcacgaa accggcgaca cgttccaggc caatgccaga atcaaggctg
     2821 tcgcggcagc aaaggccgcg cagctcccgg cgtttgccga cgattccggg ctcgtggtgg
     2881 atgcgctcga tggcgcgcct ggcattttgt ctgcgcgctg ggccggacca aacaaggact
     2941 tcgacgcggc catggcacag atcgaacggt tactcaggga gcgcggcgcc accacgcagg
     3001 gcaagcgcgg cgcacatttc gtttccgcgc tttgcgtcgc ctggcccgac gatcatctcg
     3061 aggaggtcga ggcccgcgcc gacggcacac tggtgtggcc gccccgcgga acggccggct
     3121 tcggttacga tccgatgttc atgccggagg gattcgaccg cacctttggc gagatgacca
     3181 gcatcgagaa gcacggtctg ccgccgctcg gcctgggtct ctcgcaccgt gcccgcgcct
     3241 tcctgaagct cgcggagatc tgccttgacc cgcgctgaga cggcagcctt cggcgtctat
     3301 gtgcactggc cgttctgcct gtcgaaatgt ccctattgcg atttcaacag ccatgtccgg
     3361 catgctgcga tcgacgagga tcgctttgcc cgcgcgttcg cgcgcgaaat cgaaaccacg
     3421 gccgcgcgcg tgccggg
//
LOCUS       DATLJT010001081         1598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_535958,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001081 DATLJT010000000
VERSION     DATLJT010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535958"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VM910_24910"
     CDS             complement(<1..160)
                     /locus_tag="VM910_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVI65797.1"
                     /translation="MATVHNLGEGPIRRPNRAPPAATKDGPRVNDDIRNAQIQLIDQN
                     GQNRGTVET"
     gene            516..1307
                     /locus_tag="VM910_24915"
     CDS             516..1307
                     /locus_tag="VM910_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI65798.1"
                     /translation="MTDFPGPALEPAYIEVGEGHSARRIAVRARAGRAPGLVWLGGFN
                     SDMKGTKALALDAWAAEHGRACIRFDYSGHGESGGKFVDGTIGRWLEESVAVFERFCR
                     GPQVMIGSSMGGWIALLLAREVARRPNDASIAGLVLIAPAPDFTEELMWKSFSPEIRE
                     EIDSKGVWFRPSQYGDGTPYPITRALIEEGRNHLLLGSSIDVGCPVHILQGARDPDVP
                     WRHAFALVQRLPAEDVVLTMIQDGDHRLSRPQDIARIIAAVSEMG"
     gene            1324..>1598
                     /locus_tag="VM910_24920"
     CDS             1324..>1598
                     /locus_tag="VM910_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI65799.1"
                     /translation="MNIPAMLRSFCDAVERRDGKAFAALFAEDGVYHDVFYGAFEGRA
                     RIAEMIDDWFYRTATDFRWDMHEPVFDGTTLYARYTFSYRSTLKEANG"
BASE COUNT          289 a          460 c          514 g          335 t
ORIGIN      
        1 cggtttcgac cgtgccgcgg ttctgtccgt tctgatcgat tagctggatc tgcgcattgc
       61 ggatatcatc attgacgcgc ggcccgtctt tggtggcagc gggcggggct ctattgggac
      121 ggcgaatggg tccttctcca aggttgtgaa cggtagcgat agtttgaagc aagacacggc
      181 aacgggcaag cgaactcgtc agttgcagcg acaaaaccga taaatgcgag gcatttcggt
      241 cacggctcgt atggctttgg ttgtgaagtc cgcatcattc tcgctgcgag ttcgtcatgc
      301 ggatttctga accaaagcca cacgagatca ataagttgct agtttccttc gatttcgaag
      361 ttcgcaaaac gggggggcca cagagcgatg cgaacttcgg aatcgggacg taagcgacat
      421 agccatcaac tttctattcc gcaagcgcgc cgaatgcctt aggtgacagt ctctttgcag
      481 gcgcagccaa gagaccgcat tggagcgact aatccatgac cgatttcccc ggtcccgcgc
      541 ttgaaccggc ctatatcgag gtgggcgagg gacattccgc gcgccggatc gcagtccggg
      601 cccgcgcggg tcgcgccccc ggtctggtct ggcttggcgg gttcaattcc gacatgaagg
      661 gcaccaaggc gctcgccctc gatgcgtggg ccgccgaaca tggccgcgcc tgcattcgct
      721 tcgattattc cggccacggc gaatccggcg gaaaattcgt cgatggaacc atcggccgct
      781 ggctcgagga gagcgtcgcg gtgttcgagc ggttttgtcg cggtccccag gtgatgatcg
      841 gttcgtccat gggcggctgg atcgcgctgt tgctggcgcg cgaggtcgca aggcgtccga
      901 acgacgcctc cattgccggc ctggtcctga tcgcgccggc tcccgacttc accgaagagc
      961 tgatgtggaa gagcttttcg cccgagatcc gtgaggagat cgacagcaaa ggcgtttggt
     1021 tcaggccctc gcaatatggc gacggcacgc cctatccgat cacgcgggcg ttgatcgagg
     1081 aaggccgcaa ccatcttttg cttggcagct ccatcgatgt cggctgtccg gtgcacatcc
     1141 tgcagggcgc gcgggacccg gacgtaccct ggcggcacgc tttcgcgctg gtccagcggt
     1201 tgccggccga agacgtggtg cttacgatga tccaggacgg cgatcatcgc ctgtcgcgtc
     1261 cgcaggacat cgcgcgtatc atcgcggcgg tttccgagat gggttgagcg acaggggaac
     1321 gccatgaaca ttcccgccat gctccggagt ttttgtgacg ccgtggagcg gcgtgacggc
     1381 aaggcgtttg cggcgctgtt cgccgaagac ggtgtctatc atgacgtgtt ctacggcgca
     1441 ttcgagggcc gtgcgagaat tgccgagatg atcgacgact ggttctatcg caccgcgacc
     1501 gattttcgct gggacatgca tgagccggtc ttcgacggta cgacgcttta tgcgcgctac
     1561 acgttcagct accgctcgac gctcaaggag gccaacgg
//
LOCUS       DATLJT010001082         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4211009,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001082 DATLJT010000000
VERSION     DATLJT010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211009"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VM910_24925"
     CDS             complement(<1..728)
                     /locus_tag="VM910_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HVI65800.1"
                     /translation="MMYVHALMGAESTPGPPVIRTIGVMDLKDALARGISDFSAMPTH
                     VFFLCLIYPIVGLILARVSLGYDVLSVLFPLAAGFALLGPFVAIGFYELSRQREQGLE
                     PSWQDAFDVLHSPSRDAIAALGFLLLAIFVFWLGVAEAIYIETFGHESAASIPNFVGE
                     LFTTRAGWTLIIVGNGIGFLFALAVLIISVVSFPLLLDRDVGAVEAVLTSVRAVAANP
                     APMAVWGLIVAGLLVVGSLPFFVGL"
     gene            966..1307
                     /locus_tag="VM910_24930"
     CDS             966..1307
                     /locus_tag="VM910_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65801.1"
                     /translation="MTDPGSAEGSYYVWVDWLDTFGLGRNVPIAMGNLNDSIYALVDG
                     KLITLCVPYPMGMFAKNVDGRIDDPNAGWKGKACDWRGRIPNCYSRPERSQYRSVRSR
                     MPVLSGKWIVS"
BASE COUNT          467 a          600 c          637 g          381 t
ORIGIN      
        1 aggccgacga agaacggcaa ggagccgacc actaggaggc cggccacgat caatccccaa
       61 accgccatgg gcgcgggatt ggcggcgacg gctcgcaccg aagtcagcac cgcttcaacg
      121 gcgccgacat cgcggtcgag cagcaacgga aatgaaacca cgctgatgat caagaccgcg
      181 agcgcgaaca ggaagccgat gccgttgccg acaatgataa gcgtccagcc ggcgcgcgtg
      241 gtgaagagtt cgccaacgaa attcggaatc gaggccgccg actcgtggcc gaaggtttca
      301 atatagatag cctctgcaac gccaagccag aacacgaaga tcgccagcag cagaaagccg
      361 agcgcggcga tggcgtcgcg agacggcgag tgcagcacgt cgaaagcgtc ctgccacgaa
      421 ggctcgagac cttgctcacg ctgccggctc aattcgtaga atccgatggc gacgaatggg
      481 ccaaggagcg cgaagccggc cgcaagggga aacagcacgg acaagacgtc atatccgagg
      541 ctcacccgcg ccagaatcag accgacgatc ggatagatca ggcagagaaa gaacacgtgg
      601 gtgggcatgg ccgagaaatc ggatattccg cgtgccagcg cgtccttgag gtccatcacg
      661 ccgatggtgc ggatcaccgg cggacccggg gtcgactctg cccccatgag agcatggaca
      721 tacatcatgt tggtgcctcc cgggaaagag gagcatcgcg ctgcgtctta tggatcacgc
      781 tgtgatcacg ccgtgccgcg acccgaaccc atattaacgc aggaaagccg ggattccccg
      841 gatgactccc gaactggcga gaatattcgc gcttttccgg ctttgtagca agactgacgc
      901 gaggtgtgga tggctaccag cagctcggtc agcccgaacg atggtcgagg ggcgggttca
      961 ggacggtgac cgatcccggc agcgcggaag gaagttacta cgtctgggtc gactggctcg
     1021 atacattcgg gctcggccgc aacgtaccga ttgccatggg caacctcaac gattcgatct
     1081 atgcgctggt ggacggcaag ctcatcactc tgtgcgtccc gtatccaatg ggcatgttcg
     1141 ccaagaacgt cgatgggcgc atcgatgatc caaacgccgg ttggaagggc aaggcatgcg
     1201 actggcgggg ccggatcccg aactgctact cgcgcccgga acggtcgcag taccgctccg
     1261 ttcgcagccg aatgcctgtc ctttctggaa aatggatagt gtcctaattt gccaaattag
     1321 gacactaccg gataatgttg ttgctggttt tgggcagact ggataatggg atgatccgca
     1381 tttggcagcc caatacggga gtagcttaga tgccgtccac cgatccaacc cccagcgtga
     1441 ctgcggatca gcttactcgg gctttaggcg aggctgttat tcggatatgg agcaatttgc
     1501 ctcaagatat gcaacttcat ctatttcagg aggcggtggc gtctcacggt gaatccataa
     1561 gatcgcagct tgcggtcttc ctgcatgaca agcatccgcg cacttcggac ccattaggca
     1621 accctcgcga aatgacggag ccggacagcc ttgggggtta gcgcgattgc ccgccgcggg
     1681 agtctcgcga tatgcaacgg cgcctgccat cgcggtcctc tccgctacat acacggtcgc
     1741 gccgttgccc cccgatcaag gcccgccggc gttgcccccg gtggaccagc ggcaagatta
     1801 ccccagccac aatagagaac attttctgat ccatgagcga tcatcccacg tttttattca
     1861 ggtagcgctc aaagggccgc cgtcgcgttg acggacatca tcaaaacggc cactcgcagc
     1921 aaagcctgag tggggcgcga ggtcatcgag tgggcggttt tttctgcccg aggatcatgc
     1981 gggagttctg catcagggcg cgattaagtt caggttggtc cagaacgtcc catagcgatt
     2041 gattgtcgtg ttcggctgat cttcgtcgga taggatgcga tttac
//
LOCUS       DATLJT010001083         3679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_993369,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001083 DATLJT010000000
VERSION     DATLJT010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993369"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VM910_24935"
     CDS             complement(<1..191)
                     /locus_tag="VM910_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HVI65802.1"
                     /translation="MTDLEGSFVTNIDSLAVHYAKPTERLLKKRLDHVNAVGRAFIAA
                     SPFLVLATGSREGLDCSPKG"
     gene            371..1495
                     /locus_tag="VM910_24940"
     CDS             371..1495
                     /locus_tag="VM910_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65803.1"
                     /translation="MKRRTLGLIGLVAYLIAAGVPAVWLFTTKAAAQSPAQARSVPVF
                     EVDQAWPKLPPQFKLGDASSFAIDAQDNVWLLHRPRTLKPEDSGKAAPAIVVFDAAGN
                     FMKAWGGAGSGYDWPEREHGIHIDHKGFVWIGGNNCPTSGLAGLKPIADDAYLKFTPD
                     GSFVLQIGRSNQSKGDVDTVNVHRAADAWVHAPTNELFVADGYGNHRVIVFDADTGAF
                     KRMWGAFGKPPGGEDSCAVVSPKTFPDAEGPAHFNVAHAIRVANDGMVYLADRENRRV
                     QMFTNDGKFVKQLIRTGTTFARNLALSADAGEQFLYVGADKGITVVDRKTLDVVGTIQ
                     PSGILGPGHHIATDSKGNIYLAQTTAGMQKLVFKGMPRTQ"
     gene            1536..1751
                     /locus_tag="VM910_24945"
     CDS             1536..1751
                     /locus_tag="VM910_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65804.1"
                     /translation="MATPVVPNQFAVGKNQIIHKPTGATFLFDPGHTDFRSVNWERAG
                     EQRLSSPDYRKDDIMRVAQQLLGKLPR"
     gene            1771..2625
                     /locus_tag="VM910_24950"
     CDS             1771..2625
                     /locus_tag="VM910_24950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65805.1"
                     /translation="MKRRRFVMLLGGAVAWPLDARTQQPTLVKRIGLMANLPLRPIEN
                     FRNRLSERGFIEGQNLMVEYRFAEGREERYADFAPSLTRVAVLGNSLNPLNRINLETA
                     HRAAQKLSIGIEGFEVRSSEEVKSALRALVDARAQTDRRDDGDLQDSGDIPVPRICRG
                     RRLHRLRPISPSCLDARPTNVDRILKGERPGNLPVQQATAFEIIVDQKAAAGLGLTLP
                     RQRSGPRRRGDRIVVMFAAARMSLPGKWPLCRDVRDHGETPRVSRPPAQHRHGAKFDQ
                     SLGSVDAA"
     gene            2647..3123
                     /locus_tag="VM910_24955"
     CDS             2647..3123
                     /locus_tag="VM910_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65806.1"
                     /translation="MSSKFACSFRCIDAVVVIGLLMLTSTIGHAFELNPAAVIYKLPN
                     QIEWKGTGGNRNAVLVGDPEKPGFYIVINKWLAGNNFSRPHFHPNDRFITVLKGTWWV
                     GTGNKFDPANTVPMPAGTFVTHFGKQVHWDGAKDEDAILLIVGEGPATSTRVEEAK"
     gene            3437..>3679
                     /locus_tag="VM910_24960"
     CDS             3437..>3679
                     /locus_tag="VM910_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65807.1"
                     /translation="MSTWTEDDKQIAARALKTLEQARVASRGAAIYCLEPVRGEMASK
                     RPDDIPERADAWLAVCSLCEALVEPDSEEPVSKWRDA"
BASE COUNT          769 a         1053 c         1126 g          731 t
ORIGIN      
        1 cccttcggcg aacagtcgag gccctctcgg cttcctgtgg cgagaaccag aaacggcgag
       61 gcggcaataa acgcgcggcc aactgcgttg acatggtcaa gccgtttttt gaggagtcgt
      121 tccgtcggct ttgcgtaatg caccgctaga ctgtcgatgt ttgttacgaa gctgccttcg
      181 agatcagtca tagcgaacct ccttccgctt tcggcgacga atgagtaagt tcctgtacaa
      241 gtgtgtatca acctgcattt gcacacaagg cagtcgggta actgccggtc ctgcgctatc
      301 gccggccgca tggcggggtg ccccgcccgc gccgacgagg tgatcgaata ggatctgaca
      361 ggaggaagcc gtgaagcgta ggactctcgg tctcattggt ctggtggcgt acctcatcgc
      421 tgctggcgtg ccagccgtat ggcttttcac taccaaggcg gctgcgcaat cgccggcaca
      481 agcgcgcagc gttccggtgt tcgaggttga ccaggcgtgg ccgaagctgc cgccgcagtt
      541 caagctcgga gatgcgtcga gcttcgccat cgacgcgcag gacaatgtct ggctcctgca
      601 ccgtccgcgc acgctcaagc ccgaagactc cggaaaggct gcacccgcga tcgtcgtgtt
      661 cgatgcagcc ggcaacttca tgaaggcctg gggcggcgcg gggagcggat acgactggcc
      721 cgagcgcgag cacggcatcc atatcgacca caaaggcttc gtgtggatcg gcggaaacaa
      781 ttgtccgacc tccggcctgg ccgggctcaa gccgattgcc gatgatgcat acttgaagtt
      841 cacgcccgac ggaagtttcg tgctgcagat cggaagaagc aaccaaagca agggcgatgt
      901 tgacacggtc aacgtgcacc gcgccgcgga cgcctgggtg catgcgccga ccaatgaatt
      961 gttcgtggcg gacggttatg gcaatcatcg tgttatcgtc ttcgatgcgg ataccggcgc
     1021 gttcaagcgg atgtggggcg ccttcggcaa gccgccgggc ggcgaggaca gctgtgcggt
     1081 cgtctcgcca aagacctttc ctgatgccga gggacccgcg catttcaatg tcgcgcatgc
     1141 catccgcgtg gccaacgatg ggatggtgta tctggccgat cgggaaaacc ggcgcgttca
     1201 aatgttcacc aacgatggca agttcgtgaa gcagctgatc agaaccggca cgacgtttgc
     1261 gcgcaatctg gcgctgtccg ccgacgctgg agagcagttc ctgtatgtgg gcgccgacaa
     1321 aggcattacc gtcgtcgatc gcaagacgct cgatgtcgtg ggcacgatcc agccgtccgg
     1381 cattctggga ccgggccatc acatcgcgac tgactccaaa ggtaatatct atctcgcgca
     1441 aaccaccgcc ggaatgcaga agcttgtatt caaagggatg ccgcggaccc aatagcagga
     1501 cgtccgctcg atctggccgc gtttaggagc aagggatggc cacgcctgtc gtgcccaatc
     1561 aatttgcagt tggcaaaaac caaatcattc acaaaccgac gggggcgacg tttctttttg
     1621 atccaggcca taccgacttc aggagcgtca actgggaacg tgccggcgag caacgattat
     1681 ctagtccaga ttaccgaaaa gacgacataa tgcgggttgc acagcagtta ttgggcaagc
     1741 tgcccagata ggccgcgatc gggtgatcga atgaagaggc gccggttcgt catgctgctc
     1801 ggcggggctg ttgcttggcc gctcgatgca cggacgcagc agccgacact ggtgaagcgt
     1861 atcgggctga tggccaatct gccgctgcgg ccgatcgaga attttcgcaa caggctgagc
     1921 gaacgcggct tcatcgaagg tcagaacctg atggtcgaat atcgtttcgc ggagggccgc
     1981 gaagagcgct acgccgattt tgctcccagc ctcacacggg tggcggtgct cggaaacagc
     2041 ctcaatccgc tcaatcgcat caatctggaa acagcgcatc gcgcggccca aaaactttcg
     2101 atcgggatcg aaggattcga agtgcgcagc agtgaagaag tgaaaagtgc gcttcgcgcg
     2161 ttggttgacg cgcgagcgca gacagatcgt cgagacgatg gcgacctaca ggattccggc
     2221 gatatacccg ttccgcgaat atgccgaggt cggcggcttc atcgcttacg gccaatatct
     2281 ccatcctgtt tggacgcgcg gccgactaac gtcgatcgca tcctgaaggg cgaacgaccc
     2341 ggcaatctcc cggttcagca ggcgacggcg ttcgagatca tcgtcgacca gaaggcagcg
     2401 gcggggctcg gcttgacgct tccccgccag cgttctggtc cgcgccgacg aggtgatcga
     2461 atagtggtga tgtttgctgc agcgcgtatg tcgcttcctg gcaaatggcc cctgtgccgc
     2521 gatgtccgtg atcatggaga gactccgagg gtaagcagac cgcccgctca gcaccgccat
     2581 ggcgctaaat tcgatcagag tttaggttca gttgacgccg cttgatgcaa ccggaggaaa
     2641 agcaccatgt cttcgaagtt tgcctgcagt ttcagatgca tcgatgcagt cgttgtaatt
     2701 ggcttgctga tgctgacctc cacaataggg cacgccttcg agctaaatcc agcagcagtc
     2761 atctataaac tgcccaatca gatcgagtgg aagggtaccg gtggtaaccg caacgcggtg
     2821 cttgtcggcg acccggaaaa gcccggcttt tacattgtga tcaacaaatg gctcgcaggt
     2881 aataacttca gtcgcccgca ttttcatccc aacgaccgct tcattaccgt gctcaagggc
     2941 acgtggtggg taggtaccgg caataaattt gatcctgcca ataccgtgcc catgccagca
     3001 ggtaccttcg tcacccattt cggcaagcag gtgcactggg atggcgccaa ggacgaggac
     3061 gcgattctcc ttattgtagg cgagggcccc gcgacaagca cgcgggtgga agaagcgaag
     3121 tagccgtttc ttccattgct cggccgcgct gacgaggtca tcgagcaggc gcactttgcc
     3181 aaaacaagtt gctgcacctg ctcaggtctc ctcatggcac ttctcggcca tccatcgttg
     3241 ttcgctgcac ggccgctttc ggtagcaatc cggacattga tcagacatcg ccgatgagcg
     3301 aggttgaccc acagcgggca tggaggagga ctgggccccc tccgaacgtg actcgtcgca
     3361 attttcggcg cggcgctggg accactcgcc cgtacccgct ctcagcgtta ttcttttcga
     3421 cagaggtgac tgtcggatgt ccacttggac cgaggatgac aagcaaattg ctgctcgcgc
     3481 tctgaagacg ctggaacagg cgcgcgtcgc atcgcgcggt gctgcaatct attgccttga
     3541 gccggtgcgg ggcgaaatgg cgagtaagcg ccctgacgat attccagaac gggcggatgc
     3601 ttggctggca gtgtgcagcc tttgcgaggc gctggtagaa ccggatagcg aggaaccggt
     3661 gtccaaatgg cgagatgcc
//
LOCUS       DATLJT010001084         3422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4441636,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001084 DATLJT010000000
VERSION     DATLJT010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4441636"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VM910_24965"
     CDS             complement(<1..212)
                     /locus_tag="VM910_24965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65808.1"
                     /translation="MPIRGLPNGVRHTRPQAGRDHPQQVVAIDRNGWSRSIVAPGRDH
                     PLRASRSKLAQDVVTQPLEPGACTRLT"
     gene            267..1781
                     /gene="istA"
                     /locus_tag="VM910_24970"
     CDS             267..1781
                     /gene="istA"
                     /locus_tag="VM910_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI65809.1"
                     /translation="MRKIRDVLRLSAAGMSKRKIAASLGVSATAAGECIRRARRAGLS
                     WPLPDDLTDEALERRLFPPPAVAAKDRRPQPNWAVVHRELRRPGVTLQLLWEEHRAVH
                     PDGYGISRYCELYRAWEARLTPTMRQSHVAGERMFVDYAGTTLAVIDASTGEAMTAQL
                     FVAVLGASSYTYAEATWTQGLSDWVGSHTRTFAFIAGAPAIVVSDNLRSGITKACFYE
                     PSVNRTYAEMAAHYSTTILPARPYRPRDKAKVEVGVQVATRFIIAKLRNRQFFSLTAL
                     NAAIAELVTQINDRMSRHLGASRRALFEELERSALKPLPAEPYVFAEWKECRVGLDYH
                     VEIEKHYYSVPHQLLRETIWARITAHTIEVVHRGQRVAAHVRSSSNRKHTTVREHMPS
                     SHRRYADWTPERLRRQADEIGRHTSALVEIILRERTHPEQGFRACVGILRLVKSYGRE
                     RLEAACSRALEIGARSYSSVNSILKNNLDRQRPATSADGPAIGHDNIRGPTYFH"
     gene            1796..2605
                     /gene="istB"
                     /locus_tag="VM910_24975"
     CDS             1796..2605
                     /gene="istB"
                     /locus_tag="VM910_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI65810.1"
                     /translation="MLTHPTLDQLRALKLDGMAQAFGELETQEETRNLTHAEWLALLL
                     DREAASRNTRRFQTRLRAARLRHSQAVIEDVDYRTPRRIDKALFQQLATCRWIAEHRG
                     LLISGPCGVGKSWLSCALAQKACRDGYTVHYARVPRLFADLDLAHGDGRFARLFRMLV
                     KVDLLVLDDWGPDRLSAAQRRDLMEIVEDRHGRGSILITSQLPVATWHEVIGEPTLGD
                     AILDRIVHNAYRLELNGPSMRKIKVAEGIETPTQPATPTATDGKPSKGAKK"
     gene            2822..>3422
                     /locus_tag="VM910_24980"
     CDS             2822..>3422
                     /locus_tag="VM910_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HVI65811.1"
                     /translation="MVAIVVVCKRLEDDASPQLYPGEAGRFVRTPWQRADHSGAPGGG
                     GVMSMMHGRMFEKVGVHCSTVHGEFAPEFRAQIPGAADDPHFYASGISLIAHMRNPNV
                     PAVHMNTRFVVTTKAWFGGGADLTPVLDRRRTQDDSDTLAFHAAMKEVCSEPNGVADY
                     DKLRTWCDEYFYLPHRKEARGVGGIFYDWHDSGDWEADFG"
BASE COUNT          606 a         1156 c         1079 g          581 t
ORIGIN      
        1 gtaaggcgcg tgcacgcgcc tggttcgagc ggctgcgtga cgacatcttg cgcgagcttt
       61 gagcggcttg ctcgcagcgg gtgatcgcga ccaggcgcta cgattgatcg cgaccacccg
      121 ttacgatcga tcgcgaccac ctgttgcggg tgatcgcgac cagcctgtgg acgggtgtga
      181 cggacgccgt tgggtaatcc gcgaatcggc atggcttcgc tccttttcca ccgggagcgg
      241 gggcaatgcc tggaccgaga ttacctatgc gcaagatccg cgacgtgctg aggctctctg
      301 ccgccggcat gtccaaacgc aagatcgccg ccagcctcgg agtgagcgcg acggccgccg
      361 gggagtgtat ccggcgggcg cggcgtgccg gcctcagctg gccgctgccg gatgacctga
      421 ctgacgaagc tctggagcgc cggctgttcc ctccgcctgc ggtcgcggcc aaggaccgcc
      481 gtccgcagcc aaactgggca gtcgtgcatc gtgagctgcg ccggcccggg gtgacgctgc
      541 agctattgtg ggaggagcat cgcgccgtcc accccgatgg ttacgggatt agtcgctact
      601 gtgagctcta tcgcgcctgg gaggcacggc tgacgccgac gatgcggcag agccacgtcg
      661 ctggcgagcg tatgtttgtc gattacgccg gcacgaccct tgcggtgatc gacgcatcga
      721 ccggcgaagc gatgacggcg cagctgttcg tcgccgtgct cggcgcgtcg agctacacct
      781 atgccgaggc cacctggacg cagggcctgt ccgactgggt cggctcgcac acgcgcacct
      841 ttgccttcat tgctggggct ccggccattg tggtgtccga caatctccgc tcgggcatca
      901 ccaaagcctg cttctacgag ccatcggtca accgcaccta cgccgagatg gcggcccact
      961 acagcaccac cattctgccg gcacggccgt atcggccccg ggacaaagct aaggtcgaag
     1021 ttggggtcca agtcgcgact cgctttatca tcgccaagct gcgcaaccgg caattcttct
     1081 cgctcacggc attgaacgcg gccattgccg agctggtcac gcagatcaat gaccgcatgt
     1141 cgcggcacct cggtgcgagc cgccgcgcgc tgtttgagga gctggagcgc tcggccctca
     1201 agccgttgcc ggccgagccc tatgtttttg ccgaatggaa ggaatgcagg gtcgggctcg
     1261 actaccacgt cgagatcgag aagcactact actcggtgcc gcaccagttg ctgcgcgaga
     1321 cgatttgggc acgcatcaca gcgcacacga tcgaagtcgt ccaccgcggc cagcgcgttg
     1381 cggctcatgt gcgctcatcc tccaatcgca agcacacgac ggtacgtgag cacatgccgt
     1441 cgagccatcg gcgctatgcc gactggacgc cggagcggct gcggcggcaa gccgatgaga
     1501 tcgggcgcca cacctcagcg ctcgtcgaga tcatcctgcg ggagcgcacg caccctgagc
     1561 aaggcttccg cgcctgcgtc ggcatcctgc ggcttgtcaa gagctatggc cgcgaacggc
     1621 tcgaagccgc ttgcagtcgt gcgctcgaga tcggtgcacg ctcctatagc tcggtcaact
     1681 cgattctgaa gaacaacctc gatcgccagc ggcctgcaac ctccgcggac gggccggcga
     1741 taggtcacga caatatccgc ggcccaacct acttccattg aggagatgac gactgatgct
     1801 gacacatccc accctcgacc agcttcgagc gctcaagctc gacggcatgg cgcaggcttt
     1861 cggcgagctc gagacacaag aggagacccg caatctcacg cacgcggagt ggttagcgct
     1921 gctgctcgac cgcgaggccg ccagccgcaa cacccggcgc ttccagaccc gactgcgtgc
     1981 tgctcggctg cgtcacagcc aagccgtgat cgaggatgtc gactaccgca cgccgcgccg
     2041 catcgacaaa gcgttgttcc agcaactggc aacgtgccgc tggatcgccg agcaccgcgg
     2101 attgctgatc agcggtccgt gcggcgtagg caagtcttgg ttgtcctgcg cgctcgcgca
     2161 gaaggcctgc cgcgatggct acaccgtcca ctacgcccgc gtgccacggc tgttcgccga
     2221 tctcgatctc gcccacggtg acggccgctt cgcgcggcta ttccgcatgc tggtcaaggt
     2281 cgatctgctt gtattggacg attggggacc cgatcgcctc tcagccgctc agcggcgcga
     2341 tctgatggaa atcgtcgagg atcgccacgg ccgcggctcc atcctcatca ccagccaact
     2401 gcccgtggca acttggcatg aagttatcgg cgagcccact ctcggggatg cgatattgga
     2461 ccgcattgtc cacaacgcct accggctcga actcaacggt ccgtccatgc gcaagatcaa
     2521 ggtcgccgaa ggaatcgaga cgccgacgca gcccgcgaca ccaaccgcaa ccgatggcaa
     2581 accgtcgaag ggagccaaga aatgacgcgc agatccgtca tcccggccgc ccccgtgggt
     2641 caacagggac ctcccgcgcg cgctgctgcg tcagctgtgg gggcactccg cagcgcgcgc
     2701 gggtccctcc tgttgactac ggggacggcc aacagcgcgc ttgacgccaa gcgccaatcc
     2761 tgagatgcac caaccttacc caacgggcgg ccatcacact ggtcgcaatc gatcgtagcg
     2821 ggtggtcgcg atcgtcgtag tgtgcaagcg gcttgaggac gacgcgtcgc cacagctcta
     2881 tccgggcgag gccggccgtt tcgtgcgcac gccctggcag cgcgccgatc acagcggcgc
     2941 acctggcggc ggcggcgtga tgtcgatgat gcatggccgc atgttcgaga aggtcggcgt
     3001 gcactgctcc accgtgcatg gcgagttcgc ccccgaattc cgcgcgcaga tccccggagc
     3061 cgcggacgat ccgcacttct acgcctccgg catctcgctg atcgcgcaca tgcgcaatcc
     3121 gaacgtgccg gccgtgcaca tgaacacgcg cttcgtcgtg accaccaagg cttggttcgg
     3181 cggcggcgcc gatctcaccc ccgtgctcga ccggcgacgt acacaggacg attcggacac
     3241 gctcgccttt cacgccgcga tgaaggaggt gtgctccgag ccgaacggcg ttgccgacta
     3301 cgacaagctc aggacctggt gcgacgaata tttctatttg ccgcaccgga aagaggcgcg
     3361 cggcgtcggc ggcatcttct acgactggca cgactccggc gactgggaag ccgacttcgg
     3421 ct
//
LOCUS       DATLJT010001085         9662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3752266,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001085 DATLJT010000000
VERSION     DATLJT010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3752266"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..752
                     /locus_tag="VM910_24985"
     CDS             249..752
                     /locus_tag="VM910_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI65812.1"
                     /translation="MRDDAVLKRNPRLDLFRFVPFRLNRLAAEVSSALSNEYQERYGL
                     DIPEWRVLATLGFRNDACSAQFISDCTRTHKSTISRAVTALMQRQIIERIENEDDRRE
                     FRLRMTRKGRALYEDLIPRLLRREQEIMSCLSAQERKDLAALLGKIEKSLDLVQTSRE
                     VDEKEAY"
     gene            785..1732
                     /locus_tag="VM910_24990"
     CDS             785..1732
                     /locus_tag="VM910_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI65813.1"
                     /translation="MMTLRQIEVVRAVMISGTISGAAKLLNVSAPGISRLIKYTERSL
                     GVRFFTRQNGRYFPTADAQMIFEQINAVYKKVDDLSDVIGKIGRGELSELRIGSVPSI
                     AQVMVPRAVEKTRRRYPDLRMDINILKIEEAIDYLLLGKGDCAAISYRLDHPALDFLP
                     LASGRLFCIVPEGHALAERKKISAAEMTRFPLIGIEPTDPYGRIMADLFARNKLPYDI
                     TIRARFGNTVCSLVQARLGIAVIDQFSVAHGNIPGVRLLEISEPTRFDTFIAIKRGAP
                     LSPYAMHFIACLRAEMEAVGHSRKQRGAENNMKLSLPHK"
     gene            1833..3248
                     /locus_tag="VM910_24995"
     CDS             1833..3248
                     /locus_tag="VM910_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65814.1"
                     /translation="MTEPSSHPTHLRQSKRAAASGWIGSALEYYDFFIYATAASLVFP
                     QIFFPSQNPTVAIIASLATYGVGYVARPIGAFVLGHWGDTHGRRNVLLLCMLVMGLST
                     MLVGVLPTYQQVGIWAPILLVALRLIQGFAVAGEISGASSMILEHAPFGHRGYYASFS
                     LQGVQAGQIFAAAVFLPLAAYLPHDAFNSWGWRVPFLMSCIVIIAAYIIRREVNETPA
                     FERAEEEGKVARTPILEAFTNSWADILRVACMALMNVIPVVAAIFGAAYAVQPAYGIG
                     FAKEIYLWIPVAGNIIAVLFIPFVGALSDRIGRRPPMIIGSLVAALLSFAYLYAVSIH
                     DVWMAIIVSLLMWGVIYQGYNAVFPSFYPELFRTRYRVSAMAIGQNIGTAISAFLPAL
                     FTAVAPPGSSDIPLKIGAITLGVAAIAAIAAFSARETYRLRLNDLGNPDATPLPKDEY
                     DRLRARTELDTGSQTPSLANG"
     gene            3278..4111
                     /locus_tag="VM910_25000"
     CDS             3278..4111
                     /locus_tag="VM910_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HVI65815.1"
                     /translation="MIDGKTKLIAHIGYPTESFKAPMIYNPWFEKCGINAVVVPMGCK
                     ADDYAAFFPLLFRLSNVMGALVTMPHKIATMALLDQVSVSAKVAGACNAVRLDGDGQL
                     IGDMFDGEGFVRGILRKGRVLSSARVLIAGSGGVGSAIAASFAKAGVDEIALFDAHAP
                     AMNGLAERIRAHYPKLKVAVGSNDPAGYDIVVNATPLGMKPGDPLPMDVARISPKTLV
                     GEVVMKQEITPFLAAARQRGCEVQVGTDMLFEQIPAYLEFFGFASTTADELRAVAKIH
                     Y"
     gene            4129..5982
                     /locus_tag="VM910_25005"
     CDS             4129..5982
                     /locus_tag="VM910_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVI65816.1"
                     /translation="MVTRSIATVSLSGTLEEKLRVIAAANFDAVEIFENDLLAFDRSP
                     RDVAMLCKELGLSICAFQPFRDFEGMPEPQRSRNFVRAERKFDLMQELGTDLLLVCSN
                     VSPASQGGIDRAAADFRELGERARSRGLRVGFEALAWGRHVSDYRDAWEIVRRADHPN
                     VGVVLDSFHILAPGFPLSAIRSIPGDRIFLVQLADAPRLELDVLSWSRHHRSFPGQGD
                     LPVGDFMDALAATGYAGPLSLEIFNDEFRAGLANSVAVDGYRSLALLEDKYALAAKLP
                     PRARTRGVGFIEFAVDRTRGSELTSLLSQLGFVLTGKHRSKSVEHWAQGDIDLVVNYE
                     QTGFAHSHYVTHGSGVCAIAMKVDDPAAVMSRAEPLKASLFRQPVGPGELEIPSIRGV
                     GGSLLYFVRGDGENWSRDFEPVTAAPKGHSLTAVDHISQSMPHGDMLSWALFYGGVLD
                     LKRLPQQEVADPVGLVQSQAFINEDESVRMVLNGSAASRTSSARFISEQLGSGVQHIA
                     FTSNDIFATVARIRAQGAALLDIPDNYYDDLEAKYDFAPGVAKALRDNHILYDRNDSG
                     EFFQAYTRAFDDRFFFEIVQRKNYSGFGAPNAAIRLAAQYREARPAALLRI"
     gene            complement(5984..6142)
                     /locus_tag="VM910_25010"
     CDS             complement(5984..6142)
                     /locus_tag="VM910_25010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65817.1"
                     /translation="MKRRDFILAAGALTACPRIVRAQQTKLARIGLARTLGIGIPNTL
                     LAAAEVID"
     gene            complement(6165..6968)
                     /locus_tag="VM910_25015"
     CDS             complement(6165..6968)
                     /locus_tag="VM910_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HVI65818.1"
                     /translation="MGNAYEIYAIRYATMARTPHMNFLLPDPHETSAQDLDYFVWLIR
                     GNGRDILVDTGFNAEEAKLRARKLTINPVDALAQFGVRAADIRDIVVTHLHYDHAGNL
                     DRFPNARFHLQDREMSYATGRCMCNGMLRHPFSVEHVTLMVRHVYGERVTFHSGDGEL
                     APGITLHRVGGHSDGLQVVRVETERGPVVLASDATHYYANLHKRSPFPIVYNIGDMAA
                     GWEIVERLAGHPDRFIPGHDPIVAEIYPRASDKVDAFALHKVPSRSFAK"
     gene            7393..8832
                     /locus_tag="VM910_25020"
     CDS             7393..8832
                     /locus_tag="VM910_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI65819.1"
                     /translation="MKIRLALLLTLIFSVLSTAPSSAAGDRYALVIGNAKYPDADAPL
                     REPINDARDVADELKRDGFTVEIGENLTGDTMRRAFDRLYGKLRPGSVALLFFSGFGI
                     QSNRQSYMIPVDAQIWSEADVRHDGFSLETVLGEINSRGAGVKIALIDASRRNPYERR
                     FRTFSAGLAPVIAPNGTLVMYSAALSSVISDNGGDHSLFVQELLKEIRVPDLMAEETL
                     NRTRVGVTRASRGEQVPWISSSLAEDFSFIPGATGGRPTIATMTPPPPPPTSPIVQPP
                     PPPQVELPPPPPPARVELPPPPPPPGVEKPVDKTQMAFADDPTIKSLTEKLDANPDDS
                     SALYRRGQVYASKGAYSLAIKDFDDTIRLNSKDVEALNNRCWARAVIGDLQAALKDCN
                     EALRLRPNFVDALDSRGLVNLKSGLTKNAITDFDAALRINPRLTSSLYGRGLAKQRSG
                     SISEGNLDIANAKAMDPNIVQEFDGYGVR"
     gene            8987..9643
                     /locus_tag="VM910_25025"
     CDS             8987..9643
                     /locus_tag="VM910_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI65820.1"
                     /translation="MLKLFGTRKFTAILSIVAIGAAFSFGVSKARAGEVATEDQIVKA
                     LVPEKKPLTRGLSAGQDEPAADPAQAKFVSSIRGRTTRSLSITEREEIATIARDKPNI
                     DLEITFDYNSADISAKSLPSVQALGRALTNPDLKGSTFVVAGHTDAAGGEAYNQDLSE
                     RRADSIKRYLMDNYHIASSDLVTVGYGKSKLKDPSQPMAEVNRRVQVVNMENKTTASK
                     "
BASE COUNT         1790 a         3090 c         2915 g         1867 t
ORIGIN      
        1 cggtcctggt ccgcgtggcg gcggtagccg aactgggtct ttgtctgatt cctcgcctgt
       61 gtttccgcct gcgccatctc ttcaaacctt cgcactagcc agacggcggc gggtttgtta
      121 aattagtctc agttgcaact atcttaaccc cgcgacgagg gtcggcgcaa cccacattgg
      181 agtgattttt gcccaaagcc cttgcaagcg acggcagcgc acgcacgcgg tccgcgaccg
      241 tcaccgcctt gcgcgatgat gccgtactca aacgaaatcc acggctggac ctgttccgtt
      301 tcgttccgtt ccgcctgaac cggctggcgg ccgaggtgag cagcgcgctg tcgaacgaat
      361 atcaggagcg ctacgggctc gacatacccg aatggcgggt gctggcgacc ctcggcttcc
      421 gcaacgatgc ttgcagcgca caatttatct ccgactgcac ccgcacgcac aagtccacca
      481 tcagccgcgc ggtgaccgcg ctgatgcagc gccagatcat cgaacgcatc gaaaatgaag
      541 acgaccggcg ggaatttcgg ctgcggatga cgcgcaaggg gcgcgcgctc tacgaggacc
      601 tgatcccccg cctgttgcgc agggagcagg agatcatgtc ctgcctttcc gcgcaggaac
      661 ggaaagatct ggcggcgctg ctcggcaaga tcgagaagag cctcgacctg gtgcagacca
      721 gccgggaggt cgacgagaaa gaggcgtatt gagccgccct gcctttgcgt tatcttggcc
      781 cgcgatgatg acgctccgac agatcgaagt cgttcgtgcc gtaatgattt ccggcacgat
      841 ctcgggtgcc gcaaagctcc tcaatgtctc ggcgccggga atcagccgcc tcatcaaata
      901 taccgagcgc tcgctcggcg tccgcttctt cacgcggcag aacggccgct acttcccgac
      961 ggccgacgcc cagatgatct tcgagcagat caacgcggtc tataaaaagg tcgacgatct
     1021 ctccgacgtc atcggcaaga tcggccgcgg tgagttgtca gagctgcgca tcggatcggt
     1081 gccgagcatt gcgcaggtga tggtgccgcg cgcggtggag aagacgcgac ggcgttatcc
     1141 cgacctgagg atggatatca acatcctgaa gatcgaggag gcgatcgact acctcctgct
     1201 cggcaagggc gattgcgcgg caatcagcta ccgactcgac catcccgcgc tcgatttcct
     1261 gccgctcgcc tcggggcggc tgttctgcat cgtgccggaa ggtcacgcgc tggccgagcg
     1321 caagaaaatt tcggccgccg agatgacgcg ctttccgctg atcggcatcg agccgaccga
     1381 cccctacggc cgcatcatgg ctgacttgtt tgcgcgcaac aaactgccct acgacatcac
     1441 gatccgcgct cgcttcggca ataccgtttg ctccctggtg caagcccgtc tcggcatcgc
     1501 cgtcatcgat cagttctcgg tggcgcatgg caacattccc ggggtgcggc ttcttgaaat
     1561 cagcgagcca acacgtttcg acaccttcat cgcgataaaa cgcggcgctc cgctttcgcc
     1621 ttatgcgatg catttcatcg cctgcctccg cgctgagatg gaagccgtcg ggcattcgcg
     1681 aaaacagcgc ggcgcagaaa ataacatgaa gttaagtttg ccgcataaat gagtaattgt
     1741 gttagctgac tgcgacgata tggttgacgg cattcgcaag aacaaaagcc cgttcaaagg
     1801 gtcgaccgca acgagtcctg gaggaagcaa acatgaccga accatcgagc cacccgaccc
     1861 atttgcgcca gtcgaagcgg gcagcggcca gcggctggat cggatccgcg ctcgaatatt
     1921 atgacttttt catctacgcg acggcagctt cgctcgtctt tccgcagatc ttctttccct
     1981 cgcaaaatcc gaccgtcgca atcatcgcat cgcttgcgac ctatggcgtc ggttatgtcg
     2041 cgcgtccgat cggcgccttc gtgctgggac attggggcga cacccacggg cggcgcaacg
     2101 tgctgctgct ctgcatgctc gtgatgggcc tttcgaccat gctggtcggc gtgctgccga
     2161 cctatcagca ggtcgggatc tgggcgccga tccttctggt ggcgctgcgg ctgatccagg
     2221 gcttcgccgt cgcgggcgaa atttccggcg cgagctcgat gatcctcgaa cacgcaccct
     2281 tcggccatcg cggctattac gcaagcttta gcttgcaagg cgtgcaggcc ggccagattt
     2341 ttgccgcggc cgtcttcctg ccgctcgcgg cctacctgcc gcatgacgcc ttcaatagct
     2401 ggggctggcg cgtcccgttc ctgatgagtt gtatcgtgat catcgccgct tacatcattc
     2461 gccgcgaggt caacgagacg ccggccttcg aacgggccga ggaagaaggc aaggttgcgc
     2521 ggacgccgat cctcgaagcc ttcacgaaca gttgggccga cattttgcgc gtcgcctgca
     2581 tggcgctgat gaacgtgatc cccgtcgtcg ccgccatctt cggcgcggcc tacgcggtgc
     2641 agcccgccta tggcatcggc tttgcgaaag agatctatct ctggattccc gtggccggaa
     2701 atatcatcgc cgttttgttc attcccttcg tcggcgccct gtcggaccgc atcggccgcc
     2761 gtccgccgat gatcatcggc tcgctggtcg cagccctcct gtcgttcgcc tatctctatg
     2821 ccgtcagcat tcacgacgtc tggatggcga tcatcgtctc gcttctgatg tggggcgtga
     2881 tctatcaggg ctacaatgcg gtattcccgt ccttctatcc ggagctgttc cgcacccgct
     2941 atcgcgtctc ggcgatggcg atcggacaaa atatcgggac ggcgatcagc gcgttcttgc
     3001 ccgcgctgtt caccgcggtg gcgcctcccg gctccagcga tattcctctg aagatcggcg
     3061 ccatcacgct cggcgtcgcc gccattgctg caattgcggc tttttcggcg cgtgaaacct
     3121 atcgcctgcg tctgaacgat ctcggcaacc ccgacgccac gccgttgccc aaggacgagt
     3181 atgatcggct gcgcgccagg accgagctcg acaccggcag ccagacccca tcgctcgcca
     3241 acggctaacg agcgagcaaa agcatcggta aatcgccatg atcgacggca aaaccaaact
     3301 catcgcgcac atcggctatc cgaccgaatc gttcaaggcg ccgatgattt acaatccctg
     3361 gttcgaaaag tgcggcatca acgcggtggt cgtgccgatg ggatgcaagg cggacgacta
     3421 tgcggccttc ttcccgctgc tgtttcgtct cagcaacgtc atgggcgcgc ttgtgaccat
     3481 gccgcacaag atcgcgacga tggcgctgct ggaccaggtc tcggtctccg ccaaggtcgc
     3541 aggcgcctgc aacgcggtgc gcctcgatgg cgacggacaa ctgatcggcg acatgttcga
     3601 cggcgaaggc tttgtccgcg gcatcctgcg caagggacgc gtcctttcct ccgcgcgcgt
     3661 tctgatcgcc ggcagcggcg gcgtcggctc ggcgatcgcc gcctcttttg ccaaggccgg
     3721 cgtcgacgag atcgcgctgt tcgatgcgca cgcaccggcc atgaacggac tggcggagcg
     3781 tatcagggcg cactatccga agctgaaagt cgcagtcggc tccaacgatc cggccggcta
     3841 tgacatcgtc gtcaatgcaa cgccgctcgg catgaagcca ggcgatccgc tgccgatgga
     3901 cgttgcgcgg atttcgccca aaacgctggt cggcgaagtc gtgatgaagc aggagatcac
     3961 gccgtttctc gccgcagcgc gccagcgcgg ttgcgaggtc caggtcggca ccgacatgct
     4021 gttcgagcaa attcctgctt atctcgagtt cttcggcttc gcgagcacga ccgccgacga
     4081 actgcgcgcg gtggcaaaga tccactacta gtcccctggg agcatgcaat ggtgacacgc
     4141 tcgatcgcca cggtctcgct cagcggcacg ctggaagaga agctgcgggt catcgctgcc
     4201 gccaatttcg acgccgtcga aatttttgaa aacgacctgc tcgccttcga tcgctcgccc
     4261 cgtgacgttg ccatgctctg caaggagctt ggcctttcga tctgcgcgtt ccagccgttc
     4321 cgcgacttcg aaggcatgcc ggagccgcag cgctcgcgga attttgtccg cgccgagcgc
     4381 aaattcgatc tgatgcagga gctggggacc gatctgctcc tcgtctgcag caatgtctcg
     4441 ccggcttcgc agggcggcat cgatcgcgcg gccgccgatt tccgcgaact cggcgagcgc
     4501 gcaaggtcgc gcgggctgcg ggtgggattc gaggcgctcg cctggggtcg gcatgtcagc
     4561 gactatcgcg acgcctggga aatcgtgcgg cgcgccgacc atccgaatgt cggcgtcgtc
     4621 cttgacagtt ttcacatcct tgcgccgggt tttccgctgt cggccatccg gtcgatcccc
     4681 ggcgaccgta tttttctcgt ccagctcgcc gatgcgccgc gtctcgagct cgacgtgctc
     4741 tcctggagcc gtcaccaccg ctcgttcccg ggccagggcg acctccctgt cggcgatttc
     4801 atggatgcgc tcgcggcgac cggttatgcc ggtccgctct cgctggagat cttcaacgac
     4861 gaatttcgcg ctgggctggc caacagcgtc gcggtcgacg gctaccgttc gcttgcgctg
     4921 ctcgaggaca aatatgcgct cgccgccaaa cttccgccgc gtgccagaac ccgcggcgtc
     4981 ggctttatcg aattcgccgt cgatcggacg cgtggcagcg agctgaccag tctgttgtcg
     5041 cagctcggct ttgtgctgac gggcaagcac cgcagcaaga gcgtcgagca ttgggcgcaa
     5101 ggagacatcg atctggtcgt gaactacgag cagaccggat ttgcccattc gcattacgtc
     5161 acgcacggct ccggagtgtg cgccatcgcc atgaaggtcg atgaccccgc cgccgtgatg
     5221 tcgcgcgcgg aaccgctgaa ggcttcattg ttccggcagc cagtcgggcc gggcgagctg
     5281 gaaattccct cgatccgcgg ggtcggcgga agcctgcttt atttcgtccg cggcgatggc
     5341 gagaactgga gccgcgattt cgaacccgtc acggctgctc ccaagggaca ttcgctcaca
     5401 gccgtcgacc atatttcgca gtcgatgccc catggcgaca tgttgtcctg ggcgttgttc
     5461 tatggcggcg tgctcgatct caagcgcctg ccgcagcagg aggtcgccga tcccgtcggc
     5521 ctcgtgcaga gccaggcctt catcaatgaa gacgagagcg tacgcatggt gctgaacggg
     5581 tcggccgcct cccgcacctc gtcggcgcgg ttcatctccg agcagcttgg ctcgggcgtc
     5641 cagcacatcg cattcacctc gaacgatatc ttcgcgacgg ttgcgcgcat acgcgcgcag
     5701 ggtgcggcgc tcctggacat ccccgacaat tattacgacg atctcgaagc caaatacgac
     5761 ttcgcgcccg gcgtggccaa agccttgcgg gacaaccaca tcctttacga tcgcaacgac
     5821 agcggcgaat ttttccaggc ctacacgcgg gcgttcgacg accggttctt cttcgagatc
     5881 gttcagcgca aaaattattc gggcttcggc gcgccgaacg cggcgatccg gcttgccgcg
     5941 caatatcgcg aggcgcgccc ggcggcgctg ttgcggatct agctcagtcg atgacctcgg
     6001 ccgcggcgag cagcgtgttc gggatgccga tgccgagcgt ccttgcgagg ccaatccgcg
     6061 cgagcttcgt ctgctgcgcg cggacgatgc gtgggcacgc cgtcaaggcg cccgccgcca
     6121 ggataaaatc ccgccgtttc atgatctcgc ccggcgcacg acggctattt cgcgaacgag
     6181 cgcgacggca ccttgtgcag ggcaaaggcg tcgaccttgt cgctggcgcg ggggtagatc
     6241 tccgcaacga tcgggtcgtg cccggggatg aaacgatcgg gatgaccggc gagccgctcg
     6301 acgatctccc atcctgcggc catgtcgccg atattgtaga cgatcggaaa cgggctgcgt
     6361 ttatgcagat tggcgtaata gtgcgtagcg tccgaagcga gcaccacagg tccgcgctcg
     6421 gtctcgaccc gcaccacctg cagcccatcg gaatggccgc cgacgcggtg caaggtgatg
     6481 ccgggcgcga gctcgccgtc gccggaatgg aaggtgacgc gttcgccgta cacatggcgc
     6541 accatcagcg tcacatgctc gaccgaaaac gggtgacgca gcatgccgtt gcacatgcag
     6601 cgcccggtcg cgtagctcat ctcgcgatcc tgcagatgga atcgcgcgtt ggggaaccga
     6661 tcaagattgc ccgcgtggtc gtaatgcaga tgcgtgacga cgatatccct gatatccgcg
     6721 gccctgaccc cgaactgcgc aagcgcatcg accggattga tcgtcagctt gcgggcacgc
     6781 agcttggcct cttccgcgtt gaagccggta tcgaccagga tatcgcggcc gttcccgcgg
     6841 atcagccaga cgaaataatc aaggtcttgc gcggaggtct cgtgcggatc gggcaggaga
     6901 aagttcatgt gcggcgtgcg cgccatcgtc gcatatcgaa tcgcatatat ttcgtaggcg
     6961 tttcccatcg ctctcgctct tctctcgttt gcagttatcc ggaggcagca gcaagccatc
     7021 gtcgactgca aaggtcaaat tcgcggagcg gatttgatgt tgccgcccgc tgcgtgtcct
     7081 tgagtcgcat gcaaagactg gatcaggatc gctcgccgtc acggagccga cgcagtctcc
     7141 gttattaatg tgaggctaat cacattaaca atgtggttct tcagcggaac cttgctgcac
     7201 cgccgggctg ccttgagcag gtgcgcgatt ctgacatgcg ggcagcgtgc gcgggagcgg
     7261 ccaagtcgcg gtcaatcgcc gcgataacca cgatgccaca agcggttgac gccccgcgac
     7321 cgtggtttga taatgccgcg tcgcgctgcg caagccctca tcggcgcgct ttttttgctg
     7381 gaccaccgag tgatgaaaat tcgccttgca ctgctcctga ctttgatctt ctcggtgctg
     7441 tcgaccgcac catcgtccgc ggcgggcgac cgctatgcac tcgtgatcgg caatgccaaa
     7501 tatccggacg cggacgcgcc gctgcgggag cccatcaacg atgcccggga tgttgcggat
     7561 gaactgaagc gcgacggctt taccgtggag atcggggaaa atctcaccgg cgacaccatg
     7621 cggcgcgcct tcgatcgcct ttacggcaag ctgcggccgg gctccgtcgc actgcttttc
     7681 ttttcggggt tcggcatcca gtccaaccgc caaagctaca tgatacccgt tgacgcccag
     7741 atctggagcg aggccgacgt gcgtcatgac ggcttcagcc tcgaaacggt gctcggcgaa
     7801 atcaacagcc gcggcgcagg cgtcaagatc gcgctgatcg atgcctccag gcgcaacccc
     7861 tatgagcgac ggttccggac gttttcggca ggccttgcac ccgtgatcgc gccgaacggc
     7921 acgctggtaa tgtactcggc ggcgctgtct tcggtgatct cggacaatgg cggcgatcac
     7981 agcctcttcg tccaggagct tctgaaggag atccgtgtcc ccgacctgat ggccgaggag
     8041 acgctcaatc gcacgcgcgt cggtgtcaca cgcgcctcgc gtggcgagca ggtgccttgg
     8101 atatcgtcct ccctggccga ggatttttcg tttatcccgg gcgcgacggg cggccgtccg
     8161 acgatcgcaa cgatgacgcc tccgccaccg ccgcctacct cgccgatcgt ccaacccccg
     8221 ccgccgccgc aagtcgagct tccgcctcct ccgccgccgg cccgggtcga gcttccgccg
     8281 ccaccaccgc cgcctggggt ggagaagccg gttgataaga cgcaaatggc gttcgccgac
     8341 gacccgacca tcaagagcct gaccgaaaaa ctggatgcca acccggacga ttccagcgcg
     8401 ctttatcgac gcggccaagt ctatgccagc aagggagcct acagcctcgc gatcaaggat
     8461 tttgacgaca cgatcaggct gaactccaag gacgtcgagg ccctcaacaa ccggtgctgg
     8521 gcgcgggcgg tgatcggcga tctgcaggcg gcgctgaagg attgcaacga agcgcttcgc
     8581 ctgcggccca atttcgtcga cgcgctggac agccggggcc tcgttaacct gaagagcggg
     8641 cttacgaaaa acgccattac cgattttgac gcagcattga gaatcaatcc ccgcctgacc
     8701 tcctcccttt acggcagggg gctcgccaag cagcgcagcg gctcgatttc agaaggcaat
     8761 ttggatatag ccaatgccaa ggccatggac cctaacatcg tccaggaatt cgacggctac
     8821 ggggtaaggt agaattaaat ttgagttggt tgagcggcgc ccccgaaccc ccggcaaggc
     8881 gccggagaca tatacgcaag tcattaggat cgcgaccaat tttgtgttgg ccgcgatccc
     8941 ccaaagcttt ttcggaaccg cgcgggcagc gcaggaggga ctggcaatgt tgaagttgtt
     9001 tggaacgcga aagtttaccg cgatcctttc gatcgtagcc attggcgccg cattctcttt
     9061 tggggtcagc aaggcgcgtg ccggcgaagt cgccaccgag gatcagatcg tgaaggcact
     9121 cgtgccggag aagaagccgc tgaccagagg cctctcggca ggccaggacg aaccggcagc
     9181 tgacccggcc caggccaagt tcgtctccag catcagggga cgaacaaccc gctcgctctc
     9241 aatcaccgag cgcgaggaga tcgcgaccat cgccagggac aagccaaata tcgatctcga
     9301 aatcaccttc gactacaact ccgcagatat cagcgccaag tcgcttcctt cggtgcaggc
     9361 gcttggccgc gcgctcacca atcccgacct gaagggctcg accttcgtgg tggccggcca
     9421 tactgatgcc gcaggcggcg aggcctacaa tcaggatctg tcggagcggc gcgccgattc
     9481 catcaagcgc tatctgatgg ataactacca catcgcatcc tccgacctcg tcaccgtcgg
     9541 atatggcaag agcaagctca aggatccgag ccagccgatg gcggaagtga atcgccgcgt
     9601 gcaggtggtg aacatggaaa acaagaccac cgcatccaag taaccgctca agtcaggggt
     9661 cg
//
LOCUS       DATLJT010001086         2967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2833837,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001086 DATLJT010000000
VERSION     DATLJT010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833837"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VM910_25030"
     CDS             <1..517
                     /locus_tag="VM910_25030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65821.1"
                     /translation="QDMRRVVTGLDDRNHAIVLFDSAMPLKDIGPGIKATNFWITDGY
                     PPNLTKEDPSGRQIGTAPPDNGTKFRVVEFAPLDAASEARLPPEMIQKGISNSPAKGI
                     PVRHPLMHRTRSLDYAVILSGEVDMMLDDTSVHLKQGDVIVQQATNHGWVNHGKEPCR
                     ILFVLMDSKEP"
     gene            complement(537..2504)
                     /locus_tag="VM910_25035"
     CDS             complement(537..2504)
                     /locus_tag="VM910_25035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI65822.1"
                     /translation="MWNFAAAWPRNHILKGHLSSGKESLTRLYGRVCKSLRQGGVAAA
                     LPADTIELDPTSSSAYINRGNAHLDAGCLDRAIADYSRAIELDPQSAIAYNNRGVTHA
                     VAGEPRAAIDDYDRAVAIDPSNAGAHHNRGDAYRELGDCERAIADYTKALALDPTCAD
                     TYCDRAAAYQARSEWDLALADYDKAIELDSNNVAAHLGRGRICARRGKFAPAIADFTR
                     AIEISPNLATAYNERGHAYLGAQNFAGSIADYAKAMELDPTIARELRAAELSLELRPG
                     TPARTSAPKPAAHDGTTITFHDYQLKPSGFYFWLVPFDPTTFGKRSAALKAAFEAYRK
                     QNAQGILDAFEDAGNDPLADLFRGIATIAKSSGADNAAELEALAERHLRAAANAGDVK
                     AKAIVGSLLATKMQGITLDIPQARELSERAAGSNDAFAIRQLAVLVSSGALGPADPER
                     AADLMWTAAELGDAVANATLAGFFSAGKGVPLDHAKAEQYLTRAADLGSTDAQNMLGD
                     LFFRRYFKKLVPTPEQGVRYFERALNCGNSTWAAHRLTGLYGCDGREYPWRSYKKAFE
                     YIPRCAPYSNPSIHFTLGATYRNNCDFVSSWAHYNVSRHLGSTDAVERLTALEQLLTQ
                     KEAQRALVLSQTIKADLKPMPPNIALQGPAR"
BASE COUNT          545 a          858 c         1001 g          563 t
ORIGIN      
        1 tcaggacatg cgccgtgtgg tcacgggcct cgatgacagg aaccatgcga tcgttctgtt
       61 cgacagcgca atgccattga aggatattgg gcccggcatc aaggcgacga atttctggat
      121 taccgacggc tatccgccga acctgacgaa ggaggaccct tccggccggc agatcgggac
      181 ggcgccgccc gacaacggga ccaagttccg tgtggtcgaa tttgcaccgc tcgatgcggc
      241 gagcgaagcc aggttaccgc ccgagatgat ccagaaaggt atcagcaact cgccggcaaa
      301 gggcatcccg gtcaggcatc cgctgatgca tcgcacgcgc agcctcgact acgccgtgat
      361 cctgtccggc gaagtcgaca tgatgctcga cgacaccagc gtgcatctca agcagggcga
      421 tgtcatcgtg cagcaggcga ccaaccacgg ctgggtcaat cacggcaagg agccgtgccg
      481 catcctgttc gtgctgatgg attcgaagga gccgtaacga ccatcgcgcg ccaatttcag
      541 cgcgcaggtc cttgaagcgc gatattgggc ggcatcggct tcaggtcggc cttgatcgtc
      601 tgcgacagta cgagcgcgcg ctgcgcttcc ttttgcgtca gaagctgttc gagggcggtc
      661 agacgttcta cggcatcggt cgaaccaagg tgccgcgaga cgttgtaatg ggcccaagag
      721 gagacgaaat cgcaattgtt gcgataggtc gcgccgagcg tgaagtggat gctgggattg
      781 gagtagggcg cacaacgggg gatgtattcg aacgccttct tgtagcttcg ccacggatac
      841 tcacggccgt cgcagccgta taggccggtg aggcggtgcg cagcccaggt ggaattgccg
      901 caattcaacg cgcgttcgaa atagcggacg ccttgttccg gagttgggac cagcttcttg
      961 aagtagcggc ggaagaagag gtcgccgagc atgttttgcg cgtcggtcga accgagatcg
     1021 gccgcgcgag tgagatattg ctccgccttg gcgtgatcca gcggcactcc cttgcccgca
     1081 ctgaagaagc cggccagcgt cgcattggcg acggcgtctc cgagttcggc ggcggtccac
     1141 atcagatcgg ccgcacgctc agggtcagca ggcccgagcg ctcctgatga caccagcacg
     1201 gcgagctggc ggatggcgaa agcgtcgttg gagccggcgg cacgttcgct gagctcgcgc
     1261 gcctgcggaa tatccagagt gatgccttgc atcttggtcg cgagcaggga gccgacgatc
     1321 gccttggcct tgacgtcacc ggcgttcgcc gcggcccgga gatgccgctc cgccagcgcc
     1381 tcgagctcgg cggcgttgtc ggcaccgctg gatttcgcga tcgttgcgat gcccctgaag
     1441 agatcggcga gcggatcgtt gccggcgtcc tcgaacgcat cgagaatgcc ctgcgcgttc
     1501 tgtttgcgat aggcctcgaa tgccgccttc agcgcagccg atcgttttcc gaacgtcgtg
     1561 gggtcgaagg gaacgagcca gaagtagaat ccgctcggtt tcaattgata gtcgtggaag
     1621 gttattgtcg tcccgtcatg cgctgcgggc tttggcgccg aagtccgcgc tggtgtaccg
     1681 gggcggagct ccagcgaaag ctccgctgcg cgcaactccc tggcaatcgt cggatcaagc
     1741 tccatggcct tggcgtaatc ggcaatcgag ccggcaaagt tttgcgcacc gagataagca
     1801 tggccgcgtt cgttataggc ggtcgcgagg ttcgggctga tttcgatcgc ccgcgtaaaa
     1861 tcggcgattg cgggagcgaa tttgccccgc ctggcgcaga tgcgcccgcg tccgagatga
     1921 gcggcaacgt tgttcgaatc aagctcgatc gccttgtcat agtctgcgag cgcgaggtcc
     1981 cactcgctgc gcgcctgata ggccgccgcc cgatcgcaat aggtgtcggc acaagtcggg
     2041 tccaatgcga gggctttggt gtaatcggca atggcgcgct cgcagtcgcc gagctcgcgg
     2101 taggcatcgc cgcggttgtg gtgcgcgccg gcgttgctgg gatcgatggc gacagcacga
     2161 tcgtaatcgt cgatcgcggc gcgcggttcg cccgcgacgg catgggtcac gccacggttg
     2221 ttgtaggcga tggcggattg tggatcgagt tcgatggccc tggagtagtc ggcgattgcc
     2281 cggtcgaggc agccagcgtc gaggtgggcg ttgccgcggt tgatgtaggc ggatgatgac
     2341 gtggggtcga gctcgatcgt gtcagcggga agggcagcgg ctacgccccc ttgacggagg
     2401 gatttgcaga cgcggccgta aagcctggtc aggctttcct ttcccgaaga caggtgtccc
     2461 ttcaggatat ggtttcgagg ccaagctgcc gcaaaattcc acattttcgt ctcccgatcg
     2521 gctcccgcaa cgcggtaatc cggtctactc ggagacagta aatcgggggc tagatcggct
     2581 gtatgacggg ggtcacgctg gcgcccgggc gtcggatttc agcttttgag ggtggggata
     2641 gcggacctcg acgcaccatg ccgttcagcc gcgccgagca acgacggcaa ctcgggccgc
     2701 ccggacgcca cgctgcgttg tatcgctcga tcaagattta cgggatcgcc ggagccgggt
     2761 tgcctggcca taaggcgaaa gaccgagacg cggcacggtg tcgccgccca atgacttccg
     2821 ccctagcaaa gcagcacttg atttccggct gcgacaagcg acaatgacgt cggcactccg
     2881 ataccgagtt ttcgagaaga cggggaaaac ccgcgcggct gcggcaatag cattgctgcg
     2941 tgggaggacg ggtatggcgt tatggtt
//
LOCUS       DATLJT010001087         1903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_75908,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001087 DATLJT010000000
VERSION     DATLJT010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75908"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..679
                     /locus_tag="VM910_25040"
     CDS             299..679
                     /locus_tag="VM910_25040"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI65823.1"
                     /translation="MPLNALHHITVQTDDLEATRDFYRDVLGLGVGFRPDLDFPGYWL
                     YCGDVPVVHLVPRGNAIGGGPSSDTGPFDHFAFLASDFQGVKSTLDNKGVKYRENHIA
                     SPPIDQLFFPDNNNVMVELNFPRD"
     gene            complement(779..1186)
                     /locus_tag="VM910_25045"
     CDS             complement(779..1186)
                     /locus_tag="VM910_25045"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI65824.1"
                     /translation="MALQFRRVVTSHDASGRAIVKIDEIAKNLVSSRLGATSCVVWTS
                     QGFPVDNTGNEDAGLRKTGTTLDNGTVFRILELAPGASPRNHRTDSIDYAVVMSGEID
                     MELDGSTVHLKAGDVLVQRGTIHNWVNRGTVPA"
     gene            1279..>1903
                     /locus_tag="VM910_25050"
     CDS             1279..>1903
                     /locus_tag="VM910_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HVI65825.1"
                     /translation="MQLRVRSITHLAEAINGFELVDSRGRDLPRFAAGAHIDLRLGEL
                     LRQYSLCNDPAERRRYCIAVLREDASRGGSRYLHDTVRVGDLVEVSAPRNNFPLDAAA
                     ERHLLIAGGIGIAPIIAMINELQRRRVDFRLHYCARSAARTPFHHDLAPLAAEGRVRF
                     HHDGGDPARGLDIGAALRDPRPGTHLYYCGPASLMAAAAEAAKEWPTG"
BASE COUNT          346 a          602 c          583 g          372 t
ORIGIN      
        1 ctttcccaaa ctttctcggt cttcagcgtg aaatcgaggt tcttctcgct gagcacgatg
       61 cggaccttgc gcgagaaggg agagagccag aggtggtaga ggacgcgcat acgctggatt
      121 gtaacgatcg cggcggtcgc tgaacacgtc cgatcgccgc gacacgcggt ttaccaacgg
      181 gtaatttgag ctcggcttgc tggcggagtc ggcgacagcg aatcggatca ttcacgcggc
      241 gtcgcggaag cgttaatatc gtttccgctg gccgaaaccc gaacgggaga atcccgcgat
      301 gccgctcaat gcacttcacc acattactgt tcagaccgac gacctcgagg cgacccgtga
      361 tttctaccgg gacgtactcg gcctcggggt aggcttccgt ccggatctcg acttccccgg
      421 ttactggctc tactgcggcg acgttccggt agtgcatctg gtgccgcggg ggaacgcgat
      481 cggcggcggt ccgtcgtcgg atacggggcc tttcgatcac ttcgcgttcc tggcgtccga
      541 ttttcagggt gtcaagtcga cgctcgacaa caaaggcgtc aagtatcgtg aaaaccacat
      601 cgcaagccca ccgatcgacc agctgttctt cccggacaac aacaacgtga tggtcgagct
      661 gaactttccg cgagactgac gcgcacccga acagatctcc gccgcgtgag ctaaccctgc
      721 gcggggagca ccttgccgcc gacctctact ggtttggcgg cgatcagcac aaaagcgatc
      781 acgcaggtac cgtgccgcga ttgacccaat tgtgaatcgt gccgcgctgc accagcacgt
      841 cgccggcctt gagatggact gtcgagccgt cgagctccat gtcgatttcg cccgacatca
      901 ccacggcata atcgatcgag tccgtacgat gatttcttgg gctggctccc ggagcgagct
      961 cgaggatgcg gaagacggtg ccgttgtcga gcgtcgtgcc ggttttgcgc agccctgcat
     1021 cctcgttgcc agtgttgtcg acaggaaagc cttggctcgt ccagacgaca caggaggtcg
     1081 cgcccagccg cgacgagacg aggttcttcg cgatttcgtc gattttgact atagcgcggc
     1141 cggacgcgtc gtggctggta acaacgcgtc ggaattgcaa agccatgatg gctctcctcc
     1201 ttggctgatg cggggcaaat cctgcgcaca ccatacaaaa ggttggcgtc tttcgagaag
     1261 ttcgggcaaa ccaagaccat gcagctgcgc gtccgctcga tcacccatct cgccgaagcc
     1321 atcaacgggt tcgaactcgt cgactcacgc ggacgcgacc tgccgcgatt tgcggccggc
     1381 gcccatatcg atttgcggct cggcgagctc ctgcggcagt actcgctgtg caacgatccc
     1441 gccgagcgtc ggcgctattg cattgcggtg ctgcgcgagg acgcgagccg tggcggctcg
     1501 cgctacctgc acgacaccgt tcgggtcggc gatctcgtcg aggtctcagc gccgcgcaac
     1561 aacttcccgc ttgatgcagc ggctgagcga catcttctga tcgccggcgg gatcggcatc
     1621 gcgccgatca tcgcgatgat caacgagctc cagcggcgcc gcgtcgattt tcggctccat
     1681 tactgcgcgc gttcggccgc aaggacgccg tttcatcacg atctggcgcc cctcgccgca
     1741 gaaggacggg tccgcttcca tcacgacggc ggcgatcctg cccgcggtct cgatatcggc
     1801 gcggcgctcc gtgatcctcg acccggaacc catctctatt attgcggtcc ggcgagcttg
     1861 atggcggccg cggccgaagc ggcgaaggaa tggccgacgg gca
//
LOCUS       DATLJT010001088         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2605137,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001088 DATLJT010000000
VERSION     DATLJT010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605137"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1346)
                     /locus_tag="VM910_25055"
     CDS             complement(369..1346)
                     /locus_tag="VM910_25055"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65826.1"
                     /translation="MTRREFITLLGGGAAAWPLAARAQRPGKIARLGYLSTANPRSVA
                     FFRAFEERLRDLGYIEGQNILVEYRNAEGNVDRLPDLAADLVRLDVNVIVTATAPATR
                     AAIRATTTIPILMVAINYDPIALGYIDSLARPGANVTGLFFQHLELVAKRFGLFKQTL
                     PSIGRVAVLSDALTADQLKAVEAANQAVGLELQPLELENPPYNFENAFRVAVGARAEA
                     IFVLESASIFRGRAEIAQLAMKNRMPASFAFRDYVEIGGLVSYGVNFSTMYRRAAEYA
                     DRILRGVKPVDLPVEQSTKFELVINLKTAKTLGLAIPPGVLAIADEVIE"
     gene            complement(1416..1715)
                     /locus_tag="VM910_25060"
     CDS             complement(1416..1715)
                     /locus_tag="VM910_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65827.1"
                     /translation="MKRRTFIGLFGGVAAAWSLAAHAQESATMGTTKATTAALPQIEV
                     ARVKPVDRMPPNLRLSDDPIDLMKDFSLSSTQGIGGAWVTPLRPSTPFDERYGQW"
     gene            complement(1712..2686)
                     /locus_tag="VM910_25065"
     CDS             complement(1712..2686)
                     /locus_tag="VM910_25065"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65828.1"
                     /translation="MTRRAFITLLGGAAAAWPLAARAQQPAKLPIVGFLGTDVSGWRP
                     WTSAFVQRLHELGWIEGRTIAIEYRWSEGRPERHAEIAAEFVRLKVDVIVSNAIAVPT
                     LMQATAVIPIVFALGLDPVGAGVVASLARPGGNVTGLSTQSTDIASKRLELLREVLPR
                     LRRLAIIGNADFPQGMLEMAEVQAVARGLGIEVVPLEIRRAEDIAPAFEALKAQADAL
                     YVVGDALVNANRTRIITFSLSARLPMIFNTRSFVQASGLMSYGPNFSDLFRRTAELVD
                     KILRGTKPGDIPVEQPSRFELVINLTTAKALGLTIPESFLLRADEVIE"
     gene            complement(2683..3663)
                     /locus_tag="VM910_25070"
     CDS             complement(2683..3663)
                     /locus_tag="VM910_25070"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65829.1"
                     /translation="MLDVRRRDFITLLGGAAAWPLVARAQQAGGIGSIGLLWPGDAPP
                     ASPRMESFRQGLRALGLVDGQNVAIELRYAQRGPQQLPELAAELIRLKVDVIFASGDL
                     APKVAQQATETIPIVAISDDILGAGVIASLSRPGGNTTGLTILSPELSAKRLEVLRDI
                     IPGLSRVTALWDPTTGASQVTMTASAALSLNLKLQVVEVRRREDVAGAVRAARNSQAE
                     ALNVFSSPFLASLHREIIAFTAEYRLPAIYQWKEHVEAGGLVSYGPNLAAIWRQSAII
                     VAKVLKGVKPADLPVEQPTKLELAMNLRTAKSLDLTIPPSVLLRADEVIE"
BASE COUNT          757 a         1140 c         1167 g          711 t
ORIGIN      
        1 cggaccatcg tccgtcggtt cgtgcagcag cgcataggcc gacgggccgg cgcctgcgga
       61 accaagcatc tcaccggcgg tgccgtggcg ctgtgatgca ggtcacactt ggagacgatt
      121 cagaactatg tgtatcaatt cggcgatttc agagacctag cccaattttt acagctaggt
      181 cggtggcgtg cgtagcaaca aaaaaggacg ccctatgacc gttcggggtc aaaagcggaa
      241 aggctcaaaa cgagcacgtc gggtccgctt tacccccgaa agcggacaat cacgcagacg
      301 cctcgctagg ttcgcttagt gccataaacg gactagtgca ttgcagcaaa caacgcgcgt
      361 gggtcgccct attcgatcac ttcgtcggcg atggcgagga cacccggcgg aatggcaagg
      421 ccgagcgttt tggcagtctt gagattgatg accaactcga atttagtcga ctgctccacc
      481 ggaagatcga ctggtttcac gccgcgtagt attctgtcgg cgtactcggc agcgcgccgg
      541 tacatcgtcg agaaatttac gccgtaggag acgagcccac cgatctccac ataatcgcgg
      601 aatgcgaagc tcgccggcat tcggtttttc atggcaagct gggcgatttc cgcgcgcccg
      661 cggaagatcg atgcagactc caacacgaaa atggcttcgg cgcgagcgcc taccgcgacg
      721 cggaacgcgt tctcgaaatt gtacggcgga ttttccaact ccagtggctg gagttccagg
      781 cctacggcct gatttgccgc ttccacagcc ttgagctggt cagccgtgag tgcatccgaa
      841 aggacggcta cccggccaat tgacggcagc gtctgcttga atagtccaaa ccgtttggcc
      901 accaattcga ggtgctggaa gaataggcct gtgacgttcg cgccgggccg agcgaggcta
      961 tcgatataac caagtgcgat cgggtcgtag ttaatcgcca ccatgagaat ggggatcgta
     1021 gtggtagctc ttatggccgc acgtgttgcg ggagcggtag ctgtgacgat aacgttcacg
     1081 tccagacgga ctaggtcggc agctaagtcg ggaagtctat cgacattccc ctcggcgttc
     1141 cggtactcga cgagtatgtt ctggccttcg atgtagccaa ggtcgcgcaa tctctcctcg
     1201 aaggcacgga agaacgccac tgagcgcggg ttcgccgtcg ataaatagcc aagccgagca
     1261 atcttgcccg gtctctgcgc gcgcgccgcg agtggccacg cagcggctcc gccgccgagc
     1321 agcgtgatga actcgcggcg cgtcattcga tcacctcgtc ggcgcgcatg ggtcgagccc
     1381 cattttgtac cacggcggcc ggggacaaag tgatttcacc attgcccgta gcgctcgtcg
     1441 aagggcgtcg acgggcgcag tggtgttacc caggctccac cgataccctg ggtgctcgaa
     1501 agactaaagt ccttcattag gtcaatcggg tcgtcgctca atctaagatt cggaggcatc
     1561 ctgtcgacag gttttacgcg cgccacttcg atttgcggca aggccgctgt cgtagctttg
     1621 gtcgtcccca tcgtcgccga ttcctgcgcg tgtgctgcaa gcgaccatgc cgcagccacg
     1681 cctccgaaca ggccgatgaa cgtgcgcctc ttcactcgat cacctcgtcg gcgcggagca
     1741 ggaacgactc cggaatggtc aggccgagtg ccttggcggt cgtgagattg atgacaagct
     1801 cgaatctgga tggctgctcg actggaatat cgccgggctt tgtaccccgc agaatcttgt
     1861 cgaccagctc ggccgtgcgc cggaacagat ccgagaagtt tgggccgtag gacatgagac
     1921 cgctcgcttg aacaaaagag cgggtattga aaatcatcgg cagacgcgcg ctgagcgaaa
     1981 acgtgatgat gcgagtacgg ttggcgttaa cgagtgcatc gcccacaacg taaagtgcgt
     2041 ccgcttgtgc cttgagcgcc tcgaacgcgg gtgcaatatc ctcggctcgc cggatttcga
     2101 gcggcacgac ctcgatgccg agaccgcgag ccacggcctg aacctcggcc atctccaaca
     2161 tgccttgggg aaagtcagca tttccgatga ttgccagccg acggagccgg gggagaacct
     2221 cacgcaagag ttcgagacgc ttgctggcaa tatcggtcga ctgggtcgat agtccagtga
     2281 cgttgccgcc tggtcgcgcc aggcttgcga ctacgcccgc gccgaccggg tctagcccca
     2341 atgcaaagac gatggggatg actgccgtcg cctgcatcaa tgtggggacg gcgatcgcat
     2401 ttgagacaat gacgtcgacc ttgaggcgga cgaactcggc tgcgatctcg gcgtggcgct
     2461 cgggacgtcc ctccgaccag cgatactcga tcgcgatagt gcgaccctcg atccacccga
     2521 gttcatgcag tcgctgcaca aaagcagaag tccagggacg ccaacccgaa acgtccgtgc
     2581 ccaagaaccc gacgatcggc agcttcgccg gctgctgcgc gcgcgccgcg agcggccatg
     2641 ccgctgccgc gccgccgagc agcgtgatga acgcgcggcg agtcattcga tcacctcgtc
     2701 ggcgcgcaag agaaccgaag gcgggatggt gagatcgaga gacttcgcgg tcctgaggtt
     2761 cattgccaac tcgagtttcg tgggttgctc gaccggtagg tcagcaggct taacgccctt
     2821 gaggaccttg gccacgatga tcgcggactg ccgccaaatc gccgcgagat tgggcccata
     2881 ggataccaga ccaccggcct cgacgtgttc cttccactga tagattgcag gaagccggta
     2941 ttccgccgtg aaggcgatga tctctcgatg cagtgacgcg agaaacggcg aagagaaaac
     3001 gttgagggcc tccgcctggg aattgcgtgc tgcacggacc gccccagcga cgtcctcacg
     3061 acgtcgcact tccacgactt gcagctttaa attcaacgac agagccgcgc tcgctgtcat
     3121 cgtcacctgc gacgcgccgg tcgtcgggtc ccagagcgca gtgacccgcg acagtccggg
     3181 gattatgtcc ctcagtacct ccagtctttt cgcgctgagt tccggggaaa ggatggtgag
     3241 cccggtagtg ttcccgccag gtcgggagag gctggcgatc accccggcac caaggatgtc
     3301 atcgctgatc gcgacaatag ggatcgtctc cgttgcttgc tgggccactt tgggcgcgag
     3361 atcgccggat gcaaaaatga cgtctacttt caaacggatc agctcagcgg caagctcggg
     3421 aagctgttgg gggcctcttt gagcgtaccg gagttcgatg gcgacgttct ggccgtcaac
     3481 caaaccaagc gcgcgaagtc cctgccgaaa cgactccatc cggggtgacg cagggggcgc
     3541 gtcgccaggc cacagcaggc cgatgctgcc aatgcccccc gcctgctgcg cccgcgccac
     3601 gagcggccat gctgccgcgc cgccgagcag cgtgatgaaa tcgcgccgtc tcacgtcgag
     3661 catcgggcgt cctcccgcgt ggcgccgccg gtcagtctac cgcatgctca agctgccgca
     3721 gaggggctgc caagtccttg gggccgacct gaattgttct gaatcgaggc ggtgt
//
LOCUS       DATLJT010001089         3501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_76185,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001089 DATLJT010000000
VERSION     DATLJT010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76185"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VM910_25075"
     CDS             <1..375
                     /locus_tag="VM910_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65830.1"
                     /translation="YMRKRKSGSIACMSSVSAQRGGGIFGGPHYSAAKAGVLGLAKAM
                     AREFGPDGIRVNCVTPGLIGTDITAGKLNDEMKTQILQGIPLGRLGEANDVAGIYTFL
                     ASDLSAYVTGAVIDVNGGMLIH"
     gene            401..1252
                     /locus_tag="VM910_25080"
     CDS             401..1252
                     /locus_tag="VM910_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVI65831.1"
                     /translation="METDRKTLANVIPLAERAHNIRRNALRMGEVQGQGYIAQALDIS
                     DVLAVAYFHAMRYRPDDPSWEGRDRFLLSNGHYAIALYAALIEAGIVPEVELETYGSD
                     ESRLPMSGMASYTPGMEMSGGSLGLGLSIAVGMGLGLKRKGSTAKVYTLFSDGELDEG
                     AVWEAIQSASHHKLDNVIGIIDVNNQQADGPSTQVMAFEPLVDKLQAFGWFVQRVDGN
                     DLDAVVAAFDAAKAYPEPKPRMIVADTLMGKGVPFLEEREKNHFIRVESHEWQLALTA
                     LEAGRQQ"
     gene            1249..2256
                     /locus_tag="VM910_25085"
     CDS             1249..2256
                     /locus_tag="VM910_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HVI65832.1"
                     /translation="MKSAKTVPSGKPRLTTSAMIASIAAEGQKTKPAPFGHALVQVAK
                     SRSDVIGMTADLGKYTDLHIFAKEFPDRYYQMGMAEQLLFGAASGLAAEGFTPFATTY
                     AVFASRRAYDFIHQTIAEEDRNVKIVCALPGLTSGYGPSHQATEDLVLFRAMPNMTVI
                     DPCDALEIEQAVPAIAAHRGPVYMRLLRGQVPLVLDEYDYKFELGQAKLIRGGKDVLI
                     ISSGIMTMRALEAAKALAGDSLDAAVLHVPTIKPLDAKTILREAGKSGRLVVVAENHT
                     VIGGLGEAVAGLLMRSGIHPAFRQIALPDEFLAAGALPTLHDRYGLSSEAVAKAVKTW
                     L"
     gene            2259..3173
                     /locus_tag="VM910_25090"
     CDS             2259..3173
                     /locus_tag="VM910_25090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI65833.1"
                     /translation="MERSQERTMKKIGFIGLGAMGRQMARNLVQRGFNVHGFDLNPAA
                     VDYLKQAGGAVAASAAKATTDADAIVLMVVNAAQAESILFDDGALAAASEGAVVCLMA
                     TCPPKTVEKLSARVQALRYRFVDAPVSGGTVGAEAASLTIMAAGEIAAFAAVHPLLEA
                     MGSRVMHVGDKPGQGAVVKTINQLLCGVHIAAAAEALAFAEKMGVDAAGVLGIMQDTA
                     AASWMLRNRGPRMLQADPEVTSAVDIFVKDLGIVLEAGRESCAALPLAALSHQLFLSV
                     SGRGDGAADDSQVIRAYHALNGQQPQGS"
     gene            3226..>3501
                     /locus_tag="VM910_25095"
     CDS             3226..>3501
                     /locus_tag="VM910_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI65834.1"
                     /translation="MSITHKGLSRRAILAGAASIPLVAILKRPAHAAEFELKYATGQD
                     PTHPVNIRAQEALNRIRETTSGRVDIKLFPANQLGSDTDLLAQVRNGS"
BASE COUNT          729 a         1051 c         1071 g          650 t
ORIGIN      
        1 tacatgcgca agcgcaagtc cggatcgatc gcatgcatgt cgtcggtctc ggcccagcgt
       61 ggcggtggca ttttcggcgg gcctcactac tccgccgcaa aagccggcgt ccttggtttg
      121 gcaaaggcga tggctcgcga gttcggaccc gatggcatcc gcgtcaattg cgtgacgccc
      181 ggtctgatcg gaaccgatat cactgctgga aagctgaacg acgagatgaa gacgcaaata
      241 cttcagggca ttccgctcgg tcgtctcggc gaggccaacg atgtcgcggg gatctacacc
      301 tttctcgctt ccgacttgtc tgcctacgtc acgggagccg tcatcgacgt caatggcggt
      361 atgttgatcc actgattgcg gagagagagg gaagacaaca atggaaacgg acagaaaaac
      421 actcgccaac gtgatcccgc tcgcagagcg agcccacaac atccgccgca acgcgctccg
      481 catgggcgag gtccaaggtc agggctacat cgcacaggcg ctcgacatct ccgacgttct
      541 cgcggtcgcc tattttcacg cgatgcgcta tcgaccggac gatccgtcct gggaggggcg
      601 cgaccggttc ctgctctcga atggacacta tgccatcgcg ctttacgccg cgttgatcga
      661 ggccggaatc gtccctgaag tcgaactcga gacctatggc agcgacgaaa gccggttgcc
      721 gatgtccggc atggcttcgt atacgccggg aatggagatg tctggtgggt cgcttggatt
      781 agggctgagc atcgccgtgg gcatgggact tggactgaag cgaaaaggct ccacggcaaa
      841 agtctatacg ctgttctccg atggagagct cgacgagggc gcggtttggg aggcgatcca
      901 gtcggcgagc caccacaagc tcgacaacgt catcggcatc atcgatgtca acaaccagca
      961 ggcggacggt ccctcgacac aagtcatggc cttcgagccg ctggtcgaca agctgcaagc
     1021 attcggctgg ttcgtccagc gggtcgacgg gaacgatctc gatgccgtcg tagcggcttt
     1081 cgatgccgcc aaagcttacc ccgagccgaa gccccgcatg atcgttgccg acacactcat
     1141 gggcaagggc gttccgttcc tcgaagagcg tgaaaagaac cactttatcc gagtcgaatc
     1201 acatgaatgg cagctagcgc tcacagcgct cgaagccggg aggcagcaat gaagtccgca
     1261 aaaaccgtac cgtccggcaa gccgcgcctg actacgtctg cgatgatcgc atcgatcgcg
     1321 gccgaagggc agaaaacgaa accggctccc ttcgggcacg ccttggtcca ggtcgcgaag
     1381 tctcgttcgg atgtgatcgg catgacagcc gacctcggca aatacaccga cctgcacata
     1441 ttcgcgaagg aattccccga tcgctactat cagatgggca tggccgaaca gcttctgttc
     1501 ggcgcagcct caggtctggc agccgagggg ttcacgccat tcgcgacgac ctacgcggtg
     1561 tttgcgtcac gccgagccta cgacttcatc caccagacga ttgcggaaga agaccgcaac
     1621 gtgaaaatcg tttgtgccct gccgggcctg acttcgggct atgggccgag ccatcaggcg
     1681 accgaggatc tcgtgctttt tcgcgccatg ccaaacatga cggtgatcga cccctgtgat
     1741 gcgctcgaaa tcgagcaagc cgtgcctgct atcgccgccc atcgggggcc ggtctatatg
     1801 cggctcctgc gaggccaggt gccgctggtc ctcgacgaat acgattacaa attcgagctt
     1861 ggccaggcca agttgatccg cggcggaaag gacgttttga tcatttcctc gggcatcatg
     1921 acgatgcgcg ccctcgaagc agcaaaggcc cttgcgggcg acagcttgga tgctgcggtc
     1981 cttcatgttc cgacgatcaa gccgctcgac gccaaaacga tactccgcga ggccggcaag
     2041 tcggggcgtc tggtcgtcgt tgctgaaaac cacaccgtga tcggtggcct tggcgaggcg
     2101 gtagcgggcc tgctgatgcg ttcaggcatt catccggctt tccggcagat tgcattgccc
     2161 gacgaatttc tcgctgccgg tgcacttccg acactgcacg acaggtacgg cctgtcgtcg
     2221 gaggcggtcg caaaagcggt caagacctgg ttgtgagcat cgaacggtcc caagagcgaa
     2281 ccatgaaaaa aattggtttc atcggactcg gagcgatggg gcgtcagatg gcgcgcaatc
     2341 tcgtccaacg cggcttcaac gttcacggct tcgatctcaa tcccgccgcg gtcgactacc
     2401 tgaagcaagc gggcggagca gttgcggctt cggccgccaa ggcaactacg gacgcagatg
     2461 cgatcgtgct gatggtcgtc aacgctgcgc aggctgagag tattttgttc gatgacggcg
     2521 cactggcagc cgcttcggaa ggcgcggtcg tgtgcctgat ggcgacctgt ccaccaaaga
     2581 cggtagaaaa actatccgct cgcgtccaag ccctgagata ccggtttgtc gatgcgccgg
     2641 tttcgggcgg cacggtcggc gcagaagcgg cctctctcac aattatggcg gcgggagaaa
     2701 tcgccgcatt cgcggccgtg catccgctgc tcgaagcgat gggaagtcgc gtcatgcacg
     2761 tcggagacaa gcctgggcag ggagccgtcg tcaagacgat caatcaactt ctctgcggtg
     2821 tccatatcgc cgcagcagcc gaggcgctcg catttgcgga aaagatgggc gtggatgcgg
     2881 ccggcgtgct cggcattatg caagatacgg cggcggcgag ctggatgctg aggaatcgcg
     2941 gaccgcgcat gttgcaagcc gatccggaag tcacgagcgc cgtcgatatc ttcgtcaagg
     3001 atctcggcat cgtcttggag gcggggcgcg agagctgcgc ggcactgccg ctggcggcac
     3061 tttcgcatca actgtttttg tccgtctccg gccgaggtga cggagctgcc gatgacagcc
     3121 aggtcatccg cgcttaccac gcgctaaacg gccaacagcc gcaagggtcc tgaacgcgtc
     3181 gcggtgggac aaccaacaac tacaagaaat caaccaggag gaaagatgtc gatcactcac
     3241 aaaggactaa gccgtcgcgc tattctcgct ggcgctgcaa gtatcccgct ggtcgccatt
     3301 ctcaagcggc ccgcgcacgc ggctgaattt gaactcaaat acgcgacggg ccaggatcct
     3361 acccatcccg tcaacatccg cgcgcaggag gcattgaatc gcattcgcga aacgacgtcc
     3421 ggtcgagtcg atatcaagct gtttcccgcg aaccaactcg gctccgacac cgatctgctc
     3481 gcgcaggtgc gcaacggcag c
//
LOCUS       DATLJT010001090        15082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4211925,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001090 DATLJT010000000
VERSION     DATLJT010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211925"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VM910_25100"
     CDS             <1..539
                     /locus_tag="VM910_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HVI65835.1"
                     /translation="MLTEQSKQLDKALLDGLERVGFRLDFGDDGTGWQFKYLSRGGGY
                     YFNVGCSDLVVSGAIRLAQFSDVDRFVAEGARLKSGDTLKADLVVLATGYRPQEELVR
                     KLFGDAVAERVGSIWGFGEEQELRNMYTRTRQAGLYFIAGSLAQCRINSKYLALQIKA
                     IEEGLLPRSVRTMEEAAA"
     gene            554..1522
                     /locus_tag="VM910_25105"
     CDS             554..1522
                     /locus_tag="VM910_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HVI65836.1"
                     /translation="MPVVLGSGEHRYRVVENWAKLPSGWTLTDVASVAVDSKDRIYVF
                     NRGEHPMIVFDRDGNFLKSWGEGLFSRGHGLHIDADDNLYCTDDGDHTVRKCTTDGKV
                     LLTIGLPGKPAPFMSGEPFHRCTHTALSPSGEIYVSDGYGNARVHKYTPDGKLIKSWG
                     EPGSDPGQFNIVHNIATDADGFVYVADRENHRVQVFDGNGRYETQWNNLHRPCALCCC
                     GGKHPNFVIGELGPGMPVNLKVPNLGPRLTIVDAKGKRVARLGGENGPGVETGKFLAP
                     HGLALDSKGDIYVGEVGVTNWKTSFPNTDMPDEVRRARCLTKLEKI"
     gene            complement(1533..2510)
                     /locus_tag="VM910_25110"
     CDS             complement(1533..2510)
                     /locus_tag="VM910_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI65837.1"
                     /translation="MRRCFGLLIAAIVGFGWSGAARAQDYPTRAITMIVPFPAGGATD
                     TLARFLGERTRAILGQPIIIENVGGAAGSLGVARAVRSPPDGYTLSIGTSTTHVLIGG
                     LYTLQFDLLKDLEPVILIGSEPLMIVGKKSLPADDLKGLIAWLKANPDKASVGIAGVG
                     ATGHLTGIAFQKATGTTFQFVPYRGNGPAMQDLVAEQIDFMIEPASNFRALLGSDSVK
                     PFAITGKTRLPSLPKIPTADEAGLPGFFASLWYGLWVPKGTAKEVIARLNAAMTQAMF
                     DPQGRRRFDELGIQISPLDQQSPEALRTLQKAEADRWWPIIKAANIKAD"
     gene            2682..3239
                     /locus_tag="VM910_25115"
     CDS             2682..3239
                     /locus_tag="VM910_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVI65838.1"
                     /translation="MNAHVSQGGWPVLVLNADFRPLSYYPLSLWSWQDAIKAVFLDRV
                     NIVEHYDRAVRSPSFEIQLPSVVSLKSFVKPTTHPAFTRFNVFLRDRFACQYCSASDD
                     LTFDHIIPRSKGGQTTWENVVAACSPCNLKKGNLTPQQARMFPRQPPYAPTVHQLHRN
                     GRLFPPNYLHDSWLDYLYWDTELDP"
     gene            complement(3242..3856)
                     /locus_tag="VM910_25120"
     CDS             complement(3242..3856)
                     /locus_tag="VM910_25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI65839.1"
                     /translation="MRPREFDHDDVLRVAFDQFWRKGVRGTSLSDIARDAGVQRGSLY
                     NAFGSKEALFLCAYERYSDEYLGSVQKALGSGTLRQHLTAFFDLTIKNFRSGSPPRGC
                     PTTRGLMEVASVEGEGLDEDARRAFADFVARIIGLVQEALSKGVERGEFDGAPEIAAL
                     HIVTVTRGLAVLERAFGDEAQLRRIAAHTINLVLGKSRGTASRR"
     gene            4145..4966
                     /locus_tag="VM910_25125"
     CDS             4145..4966
                     /locus_tag="VM910_25125"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HVI65840.1"
                     /translation="MSYRFEAGRIYRMPTHFGPAPGPRQMLDGVVYDQKRSPRRLSVA
                     ANFLTDPAALERHLPEGFTLAGEPVVTVEFHHMTDIDWLAGRGYTMIWVSWPAAFTGR
                     QDRATGRFLAVIWENLADPIITGRDEIGHPKLYAEIAEPRRWDGTQICVAGWMGFRFL
                     ELEVSQLREAEPKRPAVASGGTLMLKYTPRAGAWGEADLCQVTLTPADDPDYHIEQRH
                     TGVGTVRFHKASWSDLPTMHHVVNALAALPVIEARGGTVTLSRGGKSYRDQRVLL"
     gene            5047..5451
                     /locus_tag="VM910_25130"
     CDS             5047..5451
                     /locus_tag="VM910_25130"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI65841.1"
                     /translation="MQDRQGGCLCGAVRYVLKGDPRAIAICHCTHCQRQSGSLFSFNL
                     VIRETDYHQRGETRVYVDKGDSGQPVYRHFCGNCGSPILAKTALAPGKVVVKAGTLDN
                     MEGLQPKTEIYTDHAVKWLAPVAGAARFARNQ"
     gene            5842..6813
                     /locus_tag="VM910_25135"
     CDS             5842..6813
                     /locus_tag="VM910_25135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HVI65842.1"
                     /translation="MASLTEWKVPPAFQPRPADYAFDLDRVLSSIVGLHSIIPGDAFT
                     AETLGTERAGNGVVIDDGLVLTIGYLITEAEAVWLHLGDGRVMEGHALGFDSVTGFGL
                     VQSLGRMNIEPLSIGSSAATEIGDRVVVGGAGGRTRSVASQIAAKQEFAGYWEYLLDE
                     AIFTYPAHPNWGGTGLISKSGDLIGIGSLQLERERGGRAEHVNMIVPIDLLKPILDDL
                     RKFGKVNRPARPWLGMYSTEIDNRVVVIGIAGNGPAARAELKAGDVILAVDGEKVTSQ
                     SAFYRKLWTLGAAGVDVPLTVHHEGVTFDVVLASTDRAKLLKAPRLH"
     gene            6862..7938
                     /locus_tag="VM910_25140"
     CDS             6862..7938
                     /locus_tag="VM910_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HVI65843.1"
                     /translation="MSEAVIDDIVAVLPPLLQSLEALSFVARHLNPPDFERVMEAIGL
                     PDHALEGARSRLAAWPQEFADVRTTLETASDAALKAFAGLRAVQNGNGDLLAVFRALR
                     YAPRAQEALYPLAAKFPPVSSFFLDPALRDDPDLLARLATPARENAGIMHDNNEPGSR
                     GGFSLYVPEYYTPDRTWPLVMALHGGSGNGRNFLWSWLRDARSFGAILIAPTATGSTW
                     ALMGEDTDTPNLMRILETVRGGWNVDRERMLLTGMSDGGTFCYVSGLDGASPFTHLAP
                     VSATFHPLMAEMAEPQRLRGLPIFLVHGRLDWMFPVQVARQTQGALSAAGANVTYREI
                     EDLSHTYPREINVEMLKWLNGEGS"
     gene            8014..8220
                     /locus_tag="VM910_25145"
     CDS             8014..8220
                     /locus_tag="VM910_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65844.1"
                     /translation="MQAILGMILGAVLVIGGAYVFDSIRTSTIASGPDAQTNPTIVNW
                     DVVASDWQALKIRAHDDWVRISSK"
     gene            complement(8435..9091)
                     /locus_tag="VM910_25150"
     CDS             complement(8435..9091)
                     /locus_tag="VM910_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI65845.1"
                     /translation="MRKFADIRLQTSRRGMLTLLGAGAVVLGLSAQRASAQGREPSEA
                     AVLHDPDNPVFGNSDGDIAIVEWFDFNCPYCRKLEPELRQVVQDDGKVRLVLKDWPIL
                     GPVSVAAARIALACKYQDKYMQAHDALIGVSSRLTEPRIDEILKDAGIDLDRAKHDLV
                     ANGKAIDAMLARNNAQAEALGFRGTPSFIVGKFRVPGALTIAQFEQVIADARKAKAAN
                     "
     gene            complement(9256..9978)
                     /locus_tag="VM910_25155"
     CDS             complement(9256..9978)
                     /locus_tag="VM910_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HVI65846.1"
                     /translation="MSENPFSLWVYLSQSPLLWLTMTLVVYAGADAVSLATERHPLAN
                     PVLHSIWIVGLFLMLTGTSYPTYFAGAQFVHFLLGPATVALAVPLYENRKVVLAAILP
                     MLVALVVGSIVAIVSVVVLAEAAGLPRTVVLSLAPKSVTAGVAMGIAESLGADPSLTA
                     VSVILTGIMGAIIVTPLMNRAGITDFRARGFAAGLAAHGIGTARAFQVDAVAGVFSGI
                     AMSLNALVTSLLVPLAITLLLR"
     gene            complement(9975..10361)
                     /locus_tag="VM910_25160"
     CDS             complement(9975..10361)
                     /locus_tag="VM910_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HVI65847.1"
                     /translation="MIVSFSLILLCQLIGEVFVRGLRLPIPGPVIGLMLLLVLLFARD
                     HFSLLARGPLGDGGVENASRGLLANLSLLFIPAGVGVVQKLDLLAERGIAIVVILAIS
                     VVLTLLATVATFLVVSRLMARASEKP"
     gene            complement(10501..10935)
                     /locus_tag="VM910_25165"
     CDS             complement(10501..10935)
                     /locus_tag="VM910_25165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVI65848.1"
                     /translation="MATEATYRGTVYPWHCDHVGHMNIMWYVGKFDEANWNFFARLGL
                     TPSYLRESGRGMAAVQQQITYKRELLAGDIIEVRSTLLEVRDKSIRFVHDMRNAETGE
                     IAAICEFTGVHVDRRLRKSVVFAQPIREAALSQLAVAEPAVV"
     gene            11030..11581
                     /locus_tag="VM910_25170"
     CDS             11030..11581
                     /locus_tag="VM910_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI65849.1"
                     /translation="MTDNGEKWIKAGFAELARSGVEGVRVEVLAKNLGVTKGGFYRRF
                     KDRAALLDAMLQDWNTGRIAAIEQQASLEGETARERLEALIRLYSERMNTEGMAIELA
                     IRQWARSDAMAADAVAGVDAARLRNVAQLYRATGLANDEADAKAFLFYCFIFGQSLLF
                     LDRGQRKRAQLVAKSAERLLDEL"
     gene            complement(11736..12191)
                     /locus_tag="VM910_25175"
     CDS             complement(11736..12191)
                     /locus_tag="VM910_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65850.1"
                     /translation="MTFQAKILCAVAAVGVTALAATAPAQALTMQECSAKYKAAKDAG
                     TLNGQKWNDFRKSQCGADATAAAAPAAPAAEAKPKTPAAAPMSMGNAVFPNAVDPKYS
                     KETAGKARMHTCVDQYNANKASNGNGGMKWIQKGGGYYSECNRRLKGAA"
     gene            12375..12635
                     /locus_tag="VM910_25180"
     CDS             12375..12635
                     /locus_tag="VM910_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HVI65851.1"
                     /translation="MTITSRAAVVAIASLLFAGTASAQTTTAPASSTPAAAPAKKAEK
                     PRSPESVACSKQADEKGLHGKERKKFRSDCLKEAKAGAAKSK"
     gene            12832..13866
                     /locus_tag="VM910_25185"
     CDS             12832..13866
                     /locus_tag="VM910_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HVI65852.1"
                     /translation="MLAALETLGIGTAGGILFLWANLPGGLISGAMVATGIAAIAGRP
                     LSVPPIVTQAVLVVLGISIGSVVSRQLIQHVSAYPVTIGLLALATFCSTFGSSLYLQR
                     MHGWDRTSAFLAGSPGALSQITILAVERGADLPAIAVVQTMRVIILTAALPLVLALTG
                     IAPASSPALTFATASPPQLALLVVASLAVALLLRFLKFPASWMFGAMIASSVLHGAGL
                     VDGGVPPWVRSVALVGIGALIGSRFARMRPRVLLGHINAALGSFAIAILISSVFVAII
                     ALTTQVRLSDTVVAFAPGAMDAMLALALTLHIDPIFVGAHHLSRFVFVTIATPGIVHL
                     FGRTQDDVDD"
     gene            complement(13870..14343)
                     /locus_tag="VM910_25190"
     CDS             complement(13870..14343)
                     /locus_tag="VM910_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HVI65853.1"
                     /translation="MAALAILAIATATIAGAWFFQLVLGIEPCPMCLEQRYAYYTLIP
                     LAALVAISAARGRAPNALLLGLALLALIALANAVYGAYHAGVEWGFWKGPTSCTGSLV
                     KFGGNLLDQLDKVKVVPCDVVQWRFLGISLAGYNALISALMAAIAAWGFARTVRH"
     gene            14563..14647
                     /locus_tag="VM910_25195"
     tRNA            14563..14647
                     /locus_tag="VM910_25195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14597..14599,aa:Leu,seq:caa)
BASE COUNT         2859 a         4765 c         4632 g         2826 t
ORIGIN      
        1 tgatgctgac cgagcagtcg aaacaactcg acaaggcgct tttggatggc ctcgaacgcg
       61 tcggcttcag gctcgacttc ggcgacgacg gcaccggctg gcagttcaaa tatctcagcc
      121 gcggtggcgg ctattatttc aacgtcggct gctccgatct cgtggtcagc ggcgcgatca
      181 ggctcgcgca attttccgat gtcgaccgct ttgtcgccga aggcgcgcgg ctcaagagcg
      241 gcgatacgct caaggccgat ctggtcgtgc tggcgaccgg ctaccggccg caggaagaac
      301 tggtgcgcaa gctgttcggc gacgccgttg ccgaacgcgt cggctcgatc tggggctttg
      361 gcgaggagca ggaactgcgc aacatgtaca cccgcaccag gcaggccggg ctttatttca
      421 tcgccggcag cctcgcgcaa tgccggatca attcgaaata tctggcgctg cagatcaagg
      481 cgatcgagga aggcctgttg ccgcgatcgg tgcggacaat ggaggaggcc gcagcatgaa
      541 tgtgacgcag caggtgccgg tcgtgctcgg ctcaggcgag catcgctatc gtgtggtcga
      601 gaactgggcc aaactgccga gcggttggac tctcaccgac gtggcctcgg ttgccgtgga
      661 cagcaaggac cgcatctatg tgttcaaccg cggcgaacac ccgatgatcg tgttcgaccg
      721 cgacggcaat tttctcaaaa gctggggcga aggcctgttc tctcgtgggc acggattgca
      781 catcgacgcc gacgacaatc tctattgcac cgacgacggc gaccacaccg tgcgcaaatg
      841 cacgaccgac ggcaaggtcc tgctcacgat cggcctgccg ggcaagcctg ccccgttcat
      901 gagcggcgag ccgtttcatc gctgcaccca caccgcgttg tcgccaagcg gcgagatcta
      961 cgtgtccgac ggctacggca atgcccgcgt gcacaaatac acgcccgacg gcaaattgat
     1021 caaaagctgg ggcgagcccg gcagcgaccc cgggcaattc aacatcgtgc acaatatcgc
     1081 gaccgacgcc gacggtttcg tctacgtcgc cgaccgcgaa aatcatcggg tgcaggtgtt
     1141 cgacggcaac ggtagatatg aaactcagtg gaacaatttg caccggccct gtgcgctctg
     1201 ctgctgcggc ggcaagcatc cgaacttcgt catcggcgag cttggccccg gcatgcccgt
     1261 caatctcaaa gtccccaatc tcggcccgcg cctgaccatc gtcgacgcaa aaggaaaacg
     1321 tgtcgcgcgg ctcggcgggg agaacggccc gggcgtcgag accggaaagt tcctggcgcc
     1381 gcacgggctc gcgctcgatt ccaagggcga catctatgtc ggcgaagtcg gcgtcacgaa
     1441 ctggaaaacc agttttccca acaccgacat gccggatgaa gtccgtcgcg cgcgttgcct
     1501 gaccaagctg gagaagatct agcgacgctt cctcaatccg ccttgatgtt cgcagccttg
     1561 atgatcggcc accagcgatc cgcttcggcc ttctgcaggg tgcgcagcgc ttccggcgat
     1621 tgctgatcga gcggcgaaat ctgaatgccg agttcgtcga accgccgcct gccttgcgga
     1681 tcgaacatcg cctgcgtcat agccgcgttc agcctggcaa tgacctcctt cgccgtgccc
     1741 tttggcaccc agagcccgta ccagagcgag gcgaagaagc cgggcagccc cgcttcgtcc
     1801 gcggttggaa tcttcggcag cgatggaagc cgtgtcttgc ctgtgatcgc gaacggcttg
     1861 acgctgtcgc tgccgagcaa tgcgcggaag ttggacgcag gctcgatcat gaagtcgatc
     1921 tgctcggcca ccaggtcctg catcgcaggc ccattgccgc gataggggac aaactggaac
     1981 gtggtgccgg tcgccttctg aaacgcaatc ccggtgagat gacccgtagc gccgacgccg
     2041 gcaatgccga ccgacgcctt atcgggattg gccttgagcc aggcgatcag tcctttcaga
     2101 tcgtccgccg gcagactctt cttgccgacg atcatgagcg gctcgctgcc gatcaggatg
     2161 accggctcga gatccttcaa gagatcgaat tgcagggtgt agaggccgcc gatcagcaca
     2221 tgcgtggtcg aggtcccgat cgaaagcgta tagccgtccg gaggcgaacg gaccgcgcgg
     2281 gcgacgccga gcgagccggc cgcacctccg acattctcga tgatgatcgg ctgccccaat
     2341 attgcccgcg ttcgttcgcc gaggaaccgc gccagcgtgt cggtggcgcc gcctgcaggg
     2401 aacggaacga tcatggtgat cgctcgcgtc gggtagtcct gcgcgcgcgc cgcgccgctc
     2461 catccaaatc cgacgatggc tgcaatcagc agcccaaagc atcgacgcat acgaccacct
     2521 cccccatcga ttcgcgcccg tcaatgcggt cctgacgcgc gtgccgcgca ggacgtgttg
     2581 cgggataagc tcttctatgc tcttcacaat cccgtcacgc tgcatgtagt atgtcaatac
     2641 acgctgcttc gcagccgatc tctgggggtc aggagcgtta tttgaacgca catgtctcgc
     2701 aaggcggttg gccggtgctg gtactgaatg cggatttccg gccgctgagt tattacccgc
     2761 tgtcgctctg gtcctggcag gacgcgatca aggcggtgtt cctcgaccgc gttaacatcg
     2821 tcgagcacta tgaccgtgcg gtgcgcagcc cctccttcga gattcagctt ccgagcgtgg
     2881 tgtcgctgaa atccttcgtc aaaccgacca cgcaccccgc cttcacccgt ttcaacgttt
     2941 tcctccgcga tcgctttgcc tgccagtatt gttcggcaag cgacgatctc accttcgatc
     3001 acatcatccc gcgctccaaa ggcggccaga ccacttggga aaacgtcgtt gcagcctgct
     3061 cgccctgcaa tctgaagaag ggcaatctga cgccgcagca ggccagaatg tttcccaggc
     3121 agccgcccta tgcgcccacc gtgcatcagc tccatcgcaa cggccggctg ttcccgccga
     3181 actatctgca cgatagctgg ctcgactatc tctattggga caccgaactg gatccgtagt
     3241 ttcaccgcct cgatgctgtg ccgcgcgatt tgccgagtac gagattgatc gtgtgtgcgg
     3301 cgatcctccg gagctgggcc tcatcaccga aggcgcgttc aagcacggcc agtccgcgcg
     3361 taacggtgac aatgtgtaat gcagcgattt cgggggctcc gtcgaactcg ccgcgctcga
     3421 cgcctttgga cagagcttcc tgaaccagac cgatgatgcg agccacaaaa tccgcgaagg
     3481 ctcgccgcgc gtcctcgtcc agcccctcac cctcgaccga tgccacttcc atcagtcccc
     3541 gcgtggtcgg acatccgcgg ggcggcgagc cggaacggaa gttctttatc gtcaaatcga
     3601 aaaacgcggt gaggtgctgc cgcaaagtcc ccgagccgag cgccttttga acggagccca
     3661 gatactcgtc ggaatagcgc tcataggcgc aaagaaataa cgcttccttg ctgccgaagg
     3721 cattgtagag actgccgcgc tggacgcccg cgtcgcgcgc gatgtccgag agcgacgttc
     3781 cacgcacccc tttgcgccaa aattgatcga acgcgacgcg caagacgtcg tcgtgatcaa
     3841 actctcgcgg cctcaagttt ccctcctctg tatcgacagg tccaaacctg gctccaacgc
     3901 gcgccaaaag tatttgacac gtcgtcaaga acatgatttt agacattgtg tcaagaatct
     3961 accggggttg atagctcatt tcgtcgatga ggtgaaccac cggcgcggga gccgccattg
     4021 cgcgtgcgcg gactccagaa gatcaacggc gacaaaaacg aacgagtcga gcgggaatga
     4081 gaacacggtc gcccaagcga tccttcatcc ggtgcggcct cttcgcgata cagggagacg
     4141 atcaatgagc tatcgattcg aggccgggcg catctatcgg atgccgacgc atttcggacc
     4201 ggcgcccgga cctcgccaga tgcttgatgg cgtcgtgtat gaccagaagc gcagtccacg
     4261 acgtctgtcc gtcgcggcga acttcctgac cgatcccgcc gcgctggaac ggcatctgcc
     4321 ggaaggtttc acgctcgccg gcgaaccggt ggtcacggtc gaatttcatc atatgactga
     4381 catcgactgg ctcgccgggc gtggctacac gatgatctgg gtgagttggc cggcggcgtt
     4441 cacaggccgc caggatcgag ccaccggcag gtttctggcc gtcatctggg agaacctcgc
     4501 cgatccgatc atcaccgggc gtgacgagat cgggcatccc aaactttacg cggagatcgc
     4561 ggagccgcgc cgatgggacg ggacccagat ctgcgtggcg ggctggatgg gattccgctt
     4621 tctcgagctc gaagtgtcgc agttgcgcga agcagaaccg aagcggccag ccgtggcgtc
     4681 gggcggaacg ctgatgctga aatacacgcc ccgcgccggc gcctggggcg aggcggattt
     4741 atgccaggtt acgctcacgc cggccgacga tccggactac catatcgaac aacgccatac
     4801 gggcgtgggc acggtgcgct tccacaaggc aagctggtcg gacctgccca caatgcatca
     4861 tgtggtgaac gcgctggccg cgttgcccgt catcgaagcg cgcggtggca cggtcacgct
     4921 ctcacgtggc ggaaaatcct accgcgatca gcgtgtgctc ctgtagcgcc gcaggcgcgt
     4981 ttcgtcacgc tattcactgc ggcagcagca gactccaagt acaattaccg tttgggagaa
     5041 gagattatgc aggatcggca aggcggctgc ttgtgcggag cggtgcgtta cgtgttgaag
     5101 ggcgatccgc gggccatcgc aatatgccat tgtacgcact gccagaggca aagcggaagc
     5161 ttattctcct tcaacctcgt cataagggaa actgactacc atcagcgggg cgagaccagg
     5221 gtctacgtgg acaagggcga cagcggccag cctgtgtatc gccatttctg cgggaactgc
     5281 gggtctccta tccttgcaaa gaccgcgttg gcgcccggaa aggtcgtggt gaaggccggc
     5341 actctcgata atatggaggg cttacagcca aagaccgaaa tatatacgga tcatgcggtg
     5401 aaatggctcg cgccggtcgc aggcgctgcg cgttttgcgc ggaatcaata accgttgagg
     5461 tgacgatgta aagtcaacgc gtgcgtccgc gttgatacct gcaatttctg gcggcggcgg
     5521 tcgccgcgag cttccactcc gcctatcgtg cactcggcct caaattcgcc ttgggtcacg
     5581 ggtccggaaa gactggactt ttcgggtaac gtgatgctgg tatgtgtctc gcacatacga
     5641 tcgcccacat catcgatcgg atattcgagc ctgcttgcgc tgattctcgg tgattgaggg
     5701 acgcgaaggc ccgcattcac gtttgacgta gccggatgac agaactgatg cgcatcccgc
     5761 gaaaaattgc taaattgctc ggacgattga cgcttcggat gcgcgcctcg ttggcggcgc
     5821 cgattgaaca ggagagccgc gatggcctct ttgaccgagt ggaaagtgcc gcccgccttt
     5881 cagccgcgcc cggctgacta cgcgttcgac ctcgaccgcg tgctttcctc gatcgttggc
     5941 ctgcactcca tcattcccgg tgacgccttt accgcggaga cgctcggcac cgagcgcgcc
     6001 ggcaatggcg ttgtgatcga cgacggattg gtgctcacca tcggctatct gatcaccgag
     6061 gccgaagcgg tctggttgca cctgggcgac ggccgcgtga tggaaggcca tgcgcttggc
     6121 ttcgattccg tcacgggctt cggtctcgtg cagtcgctcg gccgcatgaa catcgagccg
     6181 ctttcgatcg gctcgtctgc tgcgactgag atcggcgacc gcgtcgtggt cggcggggcc
     6241 ggcgggcgca cccgttcggt cgcaagccag atcgcggcca agcaggagtt tgcgggctat
     6301 tgggagtatc tgctcgacga agcgatcttc acctacccgg cgcacccgaa ttggggcggc
     6361 acggggctca tctcgaaaag cggcgatttg atcgggatcg gctcgctaca gctcgagcgg
     6421 gagcgcggcg gccgggccga gcatgtcaac atgatcgtgc cgatcgatct gttgaaaccc
     6481 atcctcgacg acttgcggaa gttcggcaag gtcaatcggc cggcacggcc gtggctcggc
     6541 atgtattcga ccgagatcga caaccgcgtc gtggtgatcg gcattgcggg caacgggccg
     6601 gccgcgcgcg ctgaactgaa ggccggcgac gtcatcctgg ccgtggatgg cgaaaaagtc
     6661 acgagccaga gcgcatttta ccgcaagctg tggacgctcg gcgctgccgg cgtcgatgtg
     6721 ccgctgaccg tgcatcacga gggcgtcacc ttcgacgtgg tgctggcctc gaccgaccgc
     6781 gccaaactgc tcaaggcccc gcggctgcat tagcgggacg caggaacccg ttccggactg
     6841 ctccaacaac aggaatgcgc gatgagcgag gccgtgatcg acgacatcgt ggccgtgctg
     6901 ccgccacttc tgcaatcgct ggaggctctc tcgttcgtcg cgcgccacct caatccgccc
     6961 gatttcgagc gcgtcatgga agcgatcggc ctgccggatc acgcgctgga gggagcgcgt
     7021 tcgcggcttg ccgcatggcc gcaggagttc gccgacgtgc ggaccacact ggagacggcg
     7081 agcgacgccg cgctcaaggc attcgccgga ctgcgcgccg tccagaacgg caacggcgat
     7141 ttgctcgcgg tcttccgcgc gctgcgctat gccccgcgcg cgcaggaagc gctctacccg
     7201 ctcgcggcca aatttccgcc ggtgagcagc ttcttcctcg atcccgcgtt gcgcgacgat
     7261 cccgatctcc tggcacgcct tgcgacaccc gcgcgcgaga acgcgggcat catgcacgac
     7321 aacaatgagc ccggcagtcg cggcggcttt tcgctttacg tgccggaata ttatacgccc
     7381 gatcgcacgt ggccgctggt catggcgctg catggcggca gcggcaacgg ccgcaacttc
     7441 ctgtggagct ggttgcgcga cgcgcgcagc ttcggcgcga tcctgatcgc gccgactgca
     7501 accggcagca cctgggcgtt gatgggcgag gataccgaca cgccaaatct catgcgcatc
     7561 ctcgagacgg tgcgtggtgg ctggaacgtc gatcgcgagc ggatgctgct gaccggcatg
     7621 agcgacggcg gcacgttctg ttacgtcagc gggctggacg gcgcatcgcc gttcactcat
     7681 cttgcgccgg tgtccgccac ctttcatccg ctgatggcgg aaatggccga gccgcagcgc
     7741 ctacgcgggt tgccgatctt tctggtgcac ggtcggctcg actggatgtt tccggtgcag
     7801 gtcgcgcgcc agacgcaggg cgcgctgtcg gctgccggcg ccaacgtgac ttaccgcgag
     7861 atcgaagacc tcagccacac ctatccgcgc gagatcaacg tggagatgct gaaatggctg
     7921 aacggcgaag gttcgtgacg aagatcatct cgttccggca acgggaacca atctgcgtca
     7981 actgcgttgt ccgccgtcac cggagggttc gacatgcagg caattctcgg gatgattttg
     8041 ggtgccgtgc tcgtgatcgg cggcgcttat gtcttcgatt cgatccgcac atccacgatc
     8101 gcgagcggcc cggacgcgca gaccaatccc accatcgtga actgggatgt cgtcgcatcc
     8161 gattggcagg cgctgaagat ccgcgcccac gatgattggg tccggatctc gtcgaaataa
     8221 tcgttccagc ttgtgatggc agaaagcgcg atggcaggtc gaatcgccat cgcgctttaa
     8281 tttattgttt gcgcatgatc tccgcgcaaa agcgttcggc ccgaagcgcg atgagagtag
     8341 gttgatattg ctgcgccgga ggtgcgctcc ctccgacgct tgccggagag gtcactcgct
     8401 cgcggacgcg gctattcgac caaaaccaat ctgtttaatt ggccgccttg gccttgcgcg
     8461 cgtccgcaat cacctgttcg aactgcgcga tggtgagcgc gccgggcacg cggaacttgc
     8521 cgacaatgaa ggatggcgtc cccctgaagc cgagcgcctc cgcctgcgca ttgttgcgcg
     8581 caagcatggc gtcgatcgcc ttgccgttcg cgacaaggtc atgcttggca cggtcgaggt
     8641 cgatgccggc gtccttgaga atttcgtcga tccgcggctc ggtcaacctc gagctcacgc
     8701 cgatcagcgc gtcgtgagcc tgcatatatt tatcctgata cttgcacgcc agcgcaatgc
     8761 gcgcggcagc gaccgatacc ggccccagga tcggccaatc cttcagcaca aggcgaacct
     8821 tgccgtcgtc ctgcacgacc tggcgcagct cgggctcgag tttccggcaa taaggacagt
     8881 tgaagtcgaa ccattcgacg atcgcgatgt caccatcgct gttcccgaaa accgggttgt
     8941 ccggatcgtg caataccgct gcctcgcttg gctctcggcc ctgggctgag gccctttgtg
     9001 cgctcaggcc aagcacgacg gcgcctgcgc cgagcagcgt cagcattccg cggcggctcg
     9061 tttggagacg gatgtctgcg aattttctca tcttttcctt tgcccatgcc gggctgaatg
     9121 attcgacgtg gagaacagcg gaacattacg tgccaacata catcggctcg gtgggaaggc
     9181 tatagaagga atcccgtcga gccgccatgc ccgccacaca aggccgcatg caacagcgcg
     9241 attgcctcga atccattacc gcagcaagag cgtgatggcg agcggcacca gaagcgaagt
     9301 cacgagcgca ttgaggctca tcgcaatgcc ggaaaagacg ccggccaccg catcgacctg
     9361 aaacgcgcgg gcggtgccga tgccgtgtgc ggcaagcccg gccgcaaaac cgcgggcgcg
     9421 gaagtcggtg atgccggcgc ggttcatcaa aggcgtgaca atgatcgcgc ccatgatccc
     9481 ggtcaggatc accgaaaccg ccgtcagcga ggggtcggcg cccagcgatt cggcaatacc
     9541 catggcaacg cccgcggtga ccgatttcgg cgcgagcgac aggacgactg tgcgcggcag
     9601 gccggccgcc tccgccagca caacgacaga gacgatcgcg acgatcgaac ccaccaccag
     9661 cgccaccagc atcggcagga tcgccgccaa gaccaccttg cgattttcgt agagcggcac
     9721 ggcaagtgcg acggtcgcgg gcccgagcag gaaatgcacg aactgcgctc cggcgaaata
     9781 agtcgggtag gacgtgccgg tcagcatcag gaacaggccg acgatccaga tcgaatgcag
     9841 tacgggattg gcgagcggat ggcgctcggt tgccaaggac accgcgtccg ccccggcata
     9901 aaccacaagc gtcatcgtga gccaaagcag cggcgattgc gaaagataaa cccacagcga
     9961 gaacggattt tcgctcatgg cttctccgac gctcgcgcca tgaggcggct cacgaccaaa
    10021 aatgtcgcga ccgtcgcaag caacgtcagc accacggaga tcgcgaggat cacgacgatg
    10081 gcgatgccgc gttcggcgag caggtcgagc ttctgcacga cgccgacacc cgccggaatg
    10141 aacagaagcg acaggttcgc aagcaggccg cggctcgcgt tttcgacacc accatcaccc
    10201 aggggtccgc gggcgagcag actgaaatga tcgcgcgcga acaacaacac caggagcagc
    10261 atcaggccga tcaccggccc cggaatcggc aggcggagcc cgcgcacgaa cacttcgccg
    10321 atcaactggc aaagcagaat gagactgaag ctcacgatca tgacgagcct gcgaccaaga
    10381 ccgctccatc aagtgatccc ggtacgcgct tgtcaattgc tcagctccgt tgcggcggca
    10441 agctcgcgcc cgcaatctcg tgcatatccg gattttcggc tcgaatcgcg cggcgcggtc
    10501 tcagacgacg gccggctcgg cgaccgcaag ctggctcaaa gctgcctcgc ggatcggttg
    10561 ggcaaacacc accgatttcc gcagccgtcg gtccacatgc acgccggtga actcgcagat
    10621 cgcggcgatc tcgccggttt ccgcgttgcg catgtcatgc acgaagcgga tcgacttgtc
    10681 gcggacctcg agcagagtgc tcctgacctc gataatgtcg ccggccagga gttcgcgctt
    10741 gtaggtgatt tgctgctgca cggcggccat gccacgcccg gactcgcgca aatagctcgg
    10801 cgtcagcccg agccgcgcaa aaaaattcca gttcgcttcg tcgaacttgc cgacatacca
    10861 catgatattc atgtggccga catggtcgca gtgccacgga tagaccgtgc cgcggtacgt
    10921 cgcctcggtt gccatctcat cctcaccgcc attgtgatac ggtagcgtat cagacggggg
    10981 acgggttagc aatacggtac cgtatcaagt tcgggtgagg cctcaagaca tgaccgacaa
    11041 cggcgaaaag tggatcaaag ccggcttcgc cgaacttgcg cgctccggcg tcgagggcgt
    11101 ccgcgtcgaa gtgctggcga aaaacctcgg cgtcaccaag ggcggcttct accggcgttt
    11161 caaggaccgc gctgcgctgc ttgacgccat gctgcaggat tggaacaccg ggcggattgc
    11221 ggccattgag cagcaggcga gcctcgaagg tgaaaccgcg cgcgagcggc ttgaagcctt
    11281 gatccggctc tactccgaac ggatgaacac cgagggtatg gcgatcgagc ttgcgatccg
    11341 gcaatgggcc cgatcagacg cgatggcagc ggatgcggtt gcgggcgtcg atgcggcgcg
    11401 gttgaggaat gtcgcccagc tctatcgcgc gacggggctt gcgaacgacg aggccgacgc
    11461 aaaagccttc ctgttctatt gcttcatctt cggccagagc ctgttgtttc tcgaccgcgg
    11521 ccagcgcaaa cgcgcccagc ttgtcgcaaa atctgcagag aggctgctgg acgaactgta
    11581 agatacgacc ggtcgctttc gttcggcgac cggccgcgcg ttgtcgtggg agcacgatcg
    11641 ctcgggtcag gccccaggca tgcctttcga gaaaccgcga cctgtcccgc tgtccaaaca
    11701 ggagcgggcc cttttccgga tcatgctcca gcgggtcagg ctgcgccctt cagccgcctg
    11761 ttgcattcag aataatatcc gccgcccttt tggatccact tcatgccgcc attgccgttg
    11821 cttgccttgt tggcattgta ctgatcgacg caggtgtgca tgcgagcctt gccggcggtc
    11881 tcctttgaat atttcggatc gaccgcattc gggaaaacgg cgttgcccat cgacatcggc
    11941 gccgccgccg gggtcttcgg cttggcttcc gcggcgggcg cggcaggcgc tgccgccgcg
    12001 gtcgcgtccg ctccgcattg cgatttgcgg aagtcattcc acttctggcc gttcagggta
    12061 ccggcgtcct tggcggcttt atatttcgcg ctgcattcct gcatcgtcag cgcctgtgcc
    12121 ggtgcggtcg ccgccaacgc ggtaacgcca accgccgcaa ccgcgcacaa tatttttgct
    12181 tgaaaggtca tccttggtct ccttgggaca gctaaacgga gctgccatgt tagcgcatgc
    12241 cccgcgaggc gacaaagctg ccggcaaccg cagcgcgaaa atatagtcag ccgggatgcc
    12301 gctttattca tgcacggttc atcatcgcgg cgcgaggttg cgcacctttc tctatttcca
    12361 aaggtgcctc catgatgacg atcacgtccc gcgcggccgt cgtagccatt gcttcgctgc
    12421 tttttgccgg cacggcttct gcgcagacaa ccaccgcgcc cgcgtcctcc acgcctgccg
    12481 cagccccggc caagaaggca gaaaagccgc gcagcccgga gtcagtcgcg tgctcgaagc
    12541 aggccgatga gaagggcctg cacggcaagg agcgcaagaa attccgatcc gattgcctga
    12601 aggaggcgaa ggccggcgcc gccaagtcga agtgatcgtc agctctctcc cggttcgaaa
    12661 atccctcttt ccttgtggga gagggtaaaa agacgtcgca ttgctgcgtc gcattagagc
    12721 atttgtaccg tgcgggcgga tttgccataa ggcagcgtga agcaaatcac ggcatccatc
    12781 acggcatcca tcacggcatt cttgggcctc gcgcctgcca gccgcaccag gatgttggcc
    12841 gcgctcgaaa cgctcgggat cggcacggcc ggcggtatcc tgtttctgtg ggcgaacctg
    12901 ccgggcgggc tcatctccgg cgcgatggtc gcaaccggca ttgccgcgat cgccggccga
    12961 ccgctttccg tgccgccgat cgtgacgcaa gccgtgcttg tcgtgcttgg catctcgatc
    13021 ggctcggtgg tgtcacggca actgattcag catgtcagcg cctatcccgt caccatcggc
    13081 ttgctggcgc ttgccacctt ctgctcgacc ttcggctcga gcctctatct tcagcgcatg
    13141 catggctggg accgcacttc ggcctttctc gccggcagcc ccggcgcgct gtcccagatc
    13201 acgatattgg cggtcgaacg cggcgcggat ctgccggcga tcgcggtggt gcagaccatg
    13261 cgtgtgatca tcctgaccgc cgccctgccg ctggtgctgg cactgaccgg cattgcgccc
    13321 gcctcctcgc cggccctcac ctttgccacc gcctcgccgc cgcagctcgc ccttctcgtc
    13381 gtcgcgtcgc ttgcggttgc gctgttgctg cggtttctga agtttccggc gagctggatg
    13441 ttcggcgcga tgatcgcctc cagcgtgctg cacggcgcgg gcctcgtcga tggcggcgtg
    13501 ccgccctggg tgcgcagcgt ggcgctggtc ggaatcggcg cgctgatcgg cagccgcttt
    13561 gcgcggatga ggccaagagt gctgctcggc cacatcaacg cggcgctcgg atcctttgcg
    13621 atcgcgatcc tgatctcttc agtcttcgtc gcaatcatcg cgctgaccac gcaggtgcgg
    13681 ctgtccgaca ccgtcgtcgc ctttgcgccc ggcgcgatgg atgcgatgct ggcattggcg
    13741 ctgacgctgc acatcgatcc gatcttcgtc ggcgcgcatc atctgtcgcg ctttgtgttc
    13801 gtcaccatcg cgacgcccgg catcgtccat ctgtttggac gcacacagga tgacgtcgac
    13861 gattaaagcc tagtgccgga ccgttcgcgc gaagccccag gccgcaatcg cagccatcaa
    13921 ggccgaaatc agagcgttgt aaccggcaag cgagatgccg agaaaccgcc attggaccac
    13981 gtcgcagggc accaccttca ccttgtcgag ctggtcaagg agatttccgc cgaatttgac
    14041 aagcgacccc gtacaactgg tcggcccctt ccagaagccc cattcgacgc cggcgtggta
    14101 ggcgccgtac acggcattgg ccaacgcgat gagcgcgagg agcgccagcc ccagaagcag
    14161 cgcattgggc gccctgccgc gcgccgcgct gattgcaacc agcgccgcca gcggaataag
    14221 ggtgtaatag gcgtagcgct gttcgagaca catcggacag ggctcgatac cgagcacgag
    14281 ctggaagaac caggctccgg cgatggttgc cgtagcgatc gccaggatcg ccagcgccgc
    14341 catcgtcacc ggattggcgt cgccgcgggt cgttcgcgga atggctgatg tctctgaggt
    14401 cacgtcggtc tcccggcagg gcagcaaaca tctaaccatg gcaagcgccg ctgtcgagac
    14461 agcgacaaac acatccgcgc gggttgaccc ggcttcgccg cccgctatag tccgcccgct
    14521 tcgcgatcca gcggttttat ttcggccgcg agatgagcga atgcccctgt ggcggaactg
    14581 gtagacgcgc tcgactcaaa atcgagttcc gcaaggagtg ctggttcgat tccggccagg
    14641 ggcaccataa ctttcgaccc acaataactt tttaccgaca atgcgtcggt ggttcgatgc
    14701 cgtgttcgac tcacgatgtc gcgccgacgc caaccaaaca atgatttcgg tgggtataat
    14761 cctgggtaga aataaaaacc ggacttctaa ggcccaaggc ccgcaaggtc gtgccgtagc
    14821 cgacccacca atgatttaca gtatcgttca atgtataccc cttgaggcac acgggcgtct
    14881 tagataccgc atcaagagca aatcggagaa tgttgagcga gttgttacga aagatcagct
    14941 ttcgtatgcc caatgaaagc tgatgtccgc ttctctaatc ggccgtcgag gtcaagcact
    15001 tttagactga ccgccactgc aatgtcgacg ccgctcacgg gctcgtgctt ctcttcggaa
    15061 tcggcacaag gcccttgtat gg
//
LOCUS       DATLJT010001091         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1685241,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001091 DATLJT010000000
VERSION     DATLJT010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685241"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VM910_25200"
     CDS             <1..241
                     /locus_tag="VM910_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65854.1"
                     /translation="VAIAAGGILILATIIGAVGFDPTVLIGAIAAVIGGTVVFGSNTS
                     TLKQTMTDMKAAEAHRTELISRMDLKVVGDGKFES"
     gene            1171..1926
                     /locus_tag="VM910_25205"
     CDS             1171..1926
                     /locus_tag="VM910_25205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65855.1"
                     /translation="MGADRSKDEVRARTLVGALECLRCGITTVQDMLGLSPLTDEQTD
                     VVVAAYREAGIRVVFSPMVWDIPPIAMVRHKDSLPPAVQEMLGNKPRSIREQLDYLEH
                     QFKRHPASGTLHSAIAPFAPQRCTPKMLQGCAELADKFDFAVYTHVYETRGQVLIARE
                     LFTDHDCSLISYMDNVGLLGPKLNIVHSVWISRQEIDRMAAVDAGIVLNQLSNFKLKS
                     GIAPIYDMRQSGVRLGLGCDNCTVAMCRAYSRR"
     gene            1878..2144
                     /locus_tag="VM910_25210"
     CDS             1878..2144
                     /locus_tag="VM910_25210"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI65856.1"
                     /translation="MRQLHGSDVQSVFQAMKMFCLIAAVSEPEPGPGLAHEVLRHATI
                     GNARTAGLAGRLGAIRPGYKADLILIDLNDIAYLPYNSAARQLV"
     gene            2381..>2839
                     /locus_tag="VM910_25215"
     CDS             2381..>2839
                     /locus_tag="VM910_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65857.1"
                     /translation="MTSGVSGPATVAYASRDVVSGRSDVDVLRAFAIAVGICWSVLFV
                     VIGLRYELQMYADGSIFSYAIAARDAWAFHWHNISDRLFVYLFCHLPAETYVELTGDP
                     YGGIMVYGFLFFVAPLLGLTATFAADRSQGRIIFSYACLSTACLCPLVFGF"
BASE COUNT          524 a          842 c          840 g          633 t
ORIGIN      
        1 agtcgccatt gctgccggag gaatcttgat attggcaaca atcattggag cggtcggatt
       61 cgatcctacg gtcctgattg gggcgattgc ggctgttatc ggagggacgg tcgtttttgg
      121 ctcaaacacg agcactttga agcaaactat gaccgacatg aaagccgccg aggctcacag
      181 gaccgagttg atcagcagaa tggatcttaa ggtggtggga gacgggaagt tcgagagctg
      241 aacgcggtgg gatcaatcgg gtcagaacgt gcactcgaag gcgcgcctgt cgcttatgta
      301 tggcccgtcc ccgatctgca agcggatttt tccaagttag tcgatcagtc gatgcaggga
      361 ctgcgtcgac ccatctcgaa agttgcaatg gtcgtacagt cgttccgaac tcgctgactg
      421 gtcctcgcga cggaccacca gcttggtgca taatcgcggc cgtagccgcg cccgcggccg
      481 taatccgaat gctggcacca cgcagaggcg tcgtatcaac ctcggtcgcg ccggttctgg
      541 ctggccgccg ctttttgcga cttctcggaa gccgtgacag gctgcggggc tagctctgtc
      601 gtcgctgtcg tagtcggcgc ggtcgctgtg gtagttgcgg aaatgcccaa tcccgaagac
      661 gacccgaaaa gccccgagtg gcgtccgtac atcttcgccc gcgatcagtg gcattgccgc
      721 atttgcggtg accgcttcca gtactcggag acggtacgca aacacatcgc gcatgcgcac
      781 gacgaaaaca tgccgccgag catgtacgaa tacttcaggg gcgaagtttg gcagcgaaaa
      841 aaactcccat gatcttgtcc tatcgctgaa agtctagccg cgccgccgag aagggtgatg
      901 acctctcacc gtctcatgtt ggacatccag cttcctcgcg cgctgggcgg ttggtttact
      961 ctcagcctgc ctcagagctg ggactgggtc cttggggcag acctgaattc acggagtttg
     1021 tgtacagcat ctcggcatcc tcgttgggag cgagcacaag tgcgggtctg tgcctgggct
     1081 tatcaatgcg cactatcgtt cgcatgatgt gctgtgccgc ggcctattcg aagagctgcc
     1141 acaggaaata tggcttctct acccttgccg atgggagccg accgcagcaa ggacgaggtg
     1201 cgcgcacgca ccctggtggg cgcgctggag tgcctgcggt gcggcatcac gactgtccag
     1261 gacatgctgg ggctttctcc actcaccgac gaacagaccg atgtcgtcgt cgcggcctat
     1321 cgcgaggcgg gcattcgcgt ggtgttttca cccatggtct gggatattcc tcccatcgcg
     1381 atggtccgcc acaaggacag ccttccgcct gccgtccagg agatgttagg aaacaagccg
     1441 cggtcgattc gcgagcagct cgactacctc gagcaccaat tcaaacgcca tccggctagc
     1501 gggaccttgc actcggccat tgcgccgttc gcgccacagc gctgcacgcc gaagatgctc
     1561 caagggtgcg ccgagcttgc cgacaaattt gattttgccg tgtacactca tgtctatgag
     1621 acgcgcggtc aggtcctcat tgcgcgcgaa ctattcacgg accatgactg ctcgctgatc
     1681 agctacatgg ataacgtcgg cctgctcggg cccaagctga acattgtcca tagcgtctgg
     1741 atctcccgcc aggagattga tcgcatggca gccgtcgatg caggcatcgt gcttaatcaa
     1801 ctcagcaact tcaaactcaa gagcggcatc gccccaatct acgacatgcg ccaatcaggc
     1861 gtgcggctcg ggctgggttg cgacaactgc acggtagcga tgtgcagagc gtattccagg
     1921 cgatgaagat gttttgcctc atcgctgcag tcagcgagcc ggagccgggc cccggcctcg
     1981 cgcacgaggt tctgcggcat gccactatcg gcaacgcgcg taccgccggt ctggccggtc
     2041 gcctcggcgc catccggccc ggctacaagg cggatctgat tctgatcgat ctcaatgaca
     2101 tcgcttacct gccttacaac agcgcggcgc ggcagctcgt ttagcagtcc acagcatcaa
     2161 aaccggactc atgcagcgca gtaaaagaca caagagattg cggcggcgct tcactcgatc
     2221 tacgcttttg atgggtaacg gcaaacgtgt cgtggacttt cgtcctcgta cccgttgatc
     2281 gacggcgccg tcattttggt gggctttgcc tatacccata atcgaattac ttggcctggg
     2341 ggctggccgc ggtcgtggtg ctccgaagga ggcgattgac atgacatccg gcgtatcggg
     2401 gccggctacc gttgcttatg cgtcgcggga cgtagtctcc ggccgttcgg acgtcgatgt
     2461 cttgcgcgcc tttgctattg ccgttgggat ctgttggtcg gtcctattcg tcgtgatcgg
     2521 gcttcgctac gagcttcaga tgtacgctga tggctcgatc ttttcttatg ctatcgccgc
     2581 tcgcgacgca tgggcgtttc attggcacaa catctcggac cgtctattcg tctatctgtt
     2641 ctgccatctg cccgctgaga cctacgtcga gctgaccggc gatccctacg gcggcattat
     2701 ggtctacggg tttctgtttt tcgtcgcgcc gctgctcggg ctgacggcga catttgcagc
     2761 cgaccgctcc cagggccgca tcattttcag ctacgcctgt ctctcgacag cctgtctttg
     2821 cccgctggtc ttcggcttc
//
LOCUS       DATLJT010001092         3234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1225400,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001092 DATLJT010000000
VERSION     DATLJT010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225400"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VM910_25220"
     CDS             <1..775
                     /locus_tag="VM910_25220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HVI65858.1"
                     /translation="MPPTGNCSAGSGCSHFKVSTAEVAVSAEAIKSSVKVCMFDQYGT
                     VVDMQGGLVAVAASFLKAKGWRGDPNAFVTWWRRTQFENSMIDALLHRGHTPYREIGH
                     RAVAHVMDRAGIPHTHDEVRYLVGEIEKLKPFPEVPAALAKLSTRYKLVVLSNGDPDM
                     LETAKQYHRIPFDRVISVAEAGSFKPHVATYTKAAELLGLPMDQILFVANHAFDCIGA
                     KSAGMRTAFIDRRSRPFGETPHQPDILVPTMTDLAEAIV"
     gene            complement(772..1554)
                     /locus_tag="VM910_25225"
     CDS             complement(772..1554)
                     /locus_tag="VM910_25225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI65859.1"
                     /translation="MDINGKVCVVTGGASGIGEATARAFAKAGARGVVVADLKTSREH
                     LAQVAGDIDGLAVTADVGQEEDIAALIGAAEAKYGPIDVFFSNAGLSRKGQESASDAD
                     WDVSWRVHVMSHVFAARVLVPRMLARGSGYLLNTASAAGLLASLNSMPYGVTKNAAVA
                     LAEHLAIQYGDRGIRISVLCPQSVQTGMTTPGPSAARVDGVLQPADVARMVIEAMAEE
                     RFLILSHPQVYEYMQRKASSRDRWIAGMRRLRDRIYGSQPAG"
     gene            complement(1582..2556)
                     /locus_tag="VM910_25230"
     CDS             complement(1582..2556)
                     /locus_tag="VM910_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HVI65860.1"
                     /translation="MRICIFGAGAVGSHFSVRLAQAGHDVACVMRGPHLKAVRAKGLT
                     LRVGNSEITAKVRASDDPADLGEQDLVISTLKATSVDSLAAGLKPLLAAATPVLFAQN
                     GIPWWYDIGLPKNHPPTPDLSFLDPGGALRAAVPRERIIGGVIFSSNEVIEPGLVANL
                     SPERNRLLVGECDDRLTERIEILRATLNGAAIESPPVKEIRETIWSKLLTNMSMSVLC
                     LLTGQTARAVRDEPALREVIPRLLDEANAVAQSCIADVKRVTRSGPAPEHKPSILQDY
                     ELGRAMEIDALVRAPAAFARAAGLSTPMLDLIAGLAIQKARDKGLYRA"
     gene            complement(2637..>3234)
                     /locus_tag="VM910_25235"
     CDS             complement(2637..>3234)
                     /locus_tag="VM910_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65861.1"
                     /translation="ALIVGFTFSMAVTRYDQRKNLEEAEANAIGTEYLRADLLPDDLG
                     ARTRDLLRQYLAQRIALYEETDGDIAQDINQRTAKLQNELWASVLPATTRQPTPIVAL
                     VVAGMNDVINSQGYTQAAWWNRIPAGAWALMALMAISCNLLVGYGERRKGSLILFVLP
                     TIMSIAFFLIADLDSPRGGTIRVHPQNLRATMQSLSPQ"
BASE COUNT          567 a         1051 c         1055 g          561 t
ORIGIN      
        1 gatgccgccg accggaaact gttcggccgg ctcgggctgc agccacttta aggtgtcgac
       61 agcggaggtc gccgtgagcg ccgaggccat caaatccagc gtcaaggtct gcatgttcga
      121 ccagtacggc acggtcgtgg acatgcaggg cgggctcgtc gccgtcgccg cgtccttcct
      181 gaaggcgaag ggctggcgcg gcgatcccaa tgccttcgtc acttggtggc ggcgcacgca
      241 attcgagaac tctatgatcg atgcgctgct gcatcgcggg cacacgccct atcgcgagat
      301 cgggcaccgc gcggtcgcgc atgtgatgga ccgtgccggc atcccgcata cgcatgatga
      361 ggtgcgctac ctcgtcggcg agatcgagaa gctcaagccg tttccggaag tgccggccgc
      421 gctcgcaaaa ctgagcacgc gatacaagct cgtcgtgctg tccaacggcg atcccgacat
      481 gctggagacg gccaagcagt atcaccggat tccgttcgat cgggtgatct cggtcgcgga
      541 agccggctcc ttcaagccgc acgttgcgac ctacaccaag gcggccgaac tgctcgggct
      601 gccgatggac cagatcctgt tcgtcgccaa tcacgccttc gactgcatcg gcgcaaaatc
      661 ggccgggatg cgaacggcct tcatcgaccg ccgcagccgg ccgttcggcg agacgccgca
      721 tcagcccgac attctggtgc cgaccatgac ggatctggcg gaggcgatcg tctagcctgc
      781 tggctgcgat ccatagatcc ggtcgcgcaa gcgccgcatg ccggcgatcc agcggtcgcg
      841 gctcgaagcc ttgcgctgca tgtattcgta cacctgcggg tgcgacagga tcaggaagcg
      901 ttcctccgcc atcgcctcga tcaccatgcg cgcgacgtcg gccggctgca gcacgccatc
      961 gacccgcgcc gcgctcggac cgggcgtggt catgccggtc tgcacggact gcgggcagag
     1021 cacggagatg cggatgccgc ggtcgccata ttgaatggcg agatgttcgg cgagcgccac
     1081 cgcggcattc ttggtcaccc cgtaaggcat cgagttgagc gaggcgagca gccccgccgc
     1141 cgatgcggtg ttgaggagat agccggagcc gcgcgccagc atcctgggca ccagcacgcg
     1201 cgccgcgaac acatggctca tcacatgcac ccgccagctc acgtcccagt cggcgtcgga
     1261 tgcgctctcc tgtcccttgc gcgacagccc tgcgttggag aagaacacgt cgatcgggcc
     1321 gtatttcgcc tccgcggccc cgatcagcgc cgcgatgtcc tcttcctgtc cgacgtcggc
     1381 cgttaccgcg aggccgtcga tatcgcccgc gacctgcgca aggtgctcgc gcgacgtctt
     1441 gagatcggcg accacgacgc cgcgggcgcc cgctttcgca aaggcgcgcg cggtggcttc
     1501 gccgatcccg ctcgcgccgc ccgtcaccac gcagaccttg ccgttgatgt ccatgtgtgc
     1561 ttcccgcgtt acgaaacctg tctaggcccg atagagcccc ttgtcccgcg ccttctggat
     1621 ggcaaggccc gcgatcagat cgagcatcgg cgtcgacagt cccgccgcgc gcgcgaacgc
     1681 ggccggcgcg cggaccagcg cgtcgatctc catggcgcgg ccgagctcgt aatcctgcag
     1741 gatcgagggc ttgtgctcgg gcgcagggcc ggagcgcgtg acgcgtttga cgtcggcgat
     1801 gcagctttgc gcaacggcat tggcttcgtc gagcaggcgc gggatgacct cgcgcaaggc
     1861 cggctcgtcg cgcacggcgc gtgcggtctg gccggtcaaa aggcacaaca ccgacatcga
     1921 catgttggtc aaaagcttcg accagatcgt ctcccggatc tctttgaccg gcggcgattc
     1981 gatcgcggcg ccgttgaggg ttgcgcgcag gatttcgatc cgctcggtga ggcggtcgtc
     2041 gcattcgccg accaggagcc ggttccgctc gggcgagaga tttgcgacca ggcctggctc
     2101 gatgacctcg ttcgacgaaa agatcacgcc gccgatgatg cgctccctgg ggactgcggc
     2161 acgcagcgcg ccgccgggat cgagaaagga gagatcgggc gtcggcggat ggttcttcgg
     2221 caggccgata tcgtaccacc agggaatgcc gttctgtgcg aacaagaccg gtgtggcggc
     2281 ggcgagcagc ggtttgaggc cggcggcaag actgtcgaca ctggtcgcct tcagcgtgct
     2341 gatcacgagg tcctgctcgc cgagatcggc cggatcgtcg gaagccctca ctttggcggt
     2401 gatctcggag tttccgacgc gtagcgtcag gccctttgcc cgcaccgcct tcagatgcgg
     2461 tccgcgcatc acgcaggcga cgtcatggcc ggcctgcgcc agccgaaccg aaaaatgact
     2521 gccgacggcg cccgcgccga aaatgcagat tcgcatgggg tgaaacgccc tgtcaggagc
     2581 gagtagtatg gaagctagtt tccacaagct tgcgcgggac gcaaccgatt acaacttcac
     2641 tgcggcgaca aggattgcat cgtcgcccga agattttgcg gatgcacgcg gatcgtgcct
     2701 ccgcgagggc tgtcgagatc ggcgatcagg aaaaatgcga tggacatgat ggtcggcagc
     2761 acaaaaagga tcagagagcc ctttcgacgc tcgccatacc cgaccaggag gttgcaggaa
     2821 atcgccatca aggccatcaa cgcccatgcg ccagcgggga tccggttcca ccaggcggcc
     2881 tgcgtatagc cctgcgaatt gatgacgtcg ttcatgccgg cgacgaccag cgccacgatc
     2941 ggggtcggct gcctcgtcgt tgccggaagc accgacgccc aaagctcgtt ctggagtttt
     3001 gccgtacgct gattgatatc ctgcgcgatg tccccgtcag tttcctcata gagggcgatg
     3061 cgctgagcca gatactgtct caatagatca cgggtgcgcg cgccgaggtc gtcaggcagg
     3121 aggtcggctc gcaaatattc ggtcccgatc gcgttggctt cagcctcctc caggttcttg
     3181 cgctgatcgt agcgcgtgac ggccatcgag aaggtgaagc cgacgatcag cgcc
//
LOCUS       DATLJT010001093         5426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4212032,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001093 DATLJT010000000
VERSION     DATLJT010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4212032"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VM910_25240"
     CDS             <1..401
                     /locus_tag="VM910_25240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HVI65862.1"
                     /translation="KIPSALGTPRLPIAPGAPFSIAGVGVTIRGDGKSAGIVRKADLI
                     ATGKPGTLQIRLVDPATQGARDGLSACTGDSGAPVFEEQQAGPIVVGVVSWSTGPNNG
                     AGCGGLTGVTPLTLYRDWILQTARQWGLSL"
     gene            522..2030
                     /locus_tag="VM910_25245"
     CDS             522..2030
                     /locus_tag="VM910_25245"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI65863.1"
                     /translation="MNVSAARSISASSSQATEHFDVLIVGAGISGIGGAYHLTTQLPG
                     TSFVVLESKDSFGGTWLTHRYPGIRSDSDLYTFGYRFKPWVGPPIATAEEILAYMGEV
                     IDDNDFAGHIRYKHRILKANWSSDENLWTIEAERTDTGEQVLISANFLWMCQGYYRHS
                     EGYTPEWAGMKDFKGRIIHPQTWPDDLDLKNKKVVVIGSGATAATLIPNIADDCAHVT
                     MLQRSPTFFRLGRNAIEIADQLRQLQVNEDWIHEIVRRKILFEQDAFTRRCVSEPEQV
                     TKELIGQISGILGPDYDVATHFTPRYRPWRQRIAFVPDSDMFLGIKNGKASVVTDEIE
                     RFTEKGILLKSGKTLEADIIVTATGFNLSVLGDIAFAIDGKPLDFSKTITYRGMMFTG
                     VPNMAWVFGYFRASWTLRTDLVADFVCRLLKHMKDKGAKRVEVALRPEDHNMPLLSWI
                     DPENFNPGYMMRNMHLLPKRGDKPEWQHSQDYWTEKDEFAAIDLDDAAFVYS"
     gene            complement(2037..2873)
                     /locus_tag="VM910_25250"
     CDS             complement(2037..2873)
                     /locus_tag="VM910_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVI65864.1"
                     /translation="MRVTNLMIGTTTLAVIAAGFVGLLVFQKIHGVRNQSPLRIVFNG
                     SASGLRKGGTVIFDGVPSGVIKSIRLESPNRIVAMVMLDNNAPIRKDTTVGIEFQGLT
                     GVADIALVGGAPSAPPVPLDRDGVPVLTADWSEQQSMTDTLHHVDRVIVENQAMIKDA
                     LLTFESYTASLKSKGEAIDGIIGKADAAFAGFDSTIDKITGVVPGLADGKTEELFEKL
                     QSLRELADTYKKKSAAFLEDGRRMLLDVSEAANNMTRKIDPAAVAPAPPQPPRRPQQK
                     RQ"
     gene            3016..3849
                     /locus_tag="VM910_25255"
     CDS             3016..3849
                     /locus_tag="VM910_25255"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HVI65865.1"
                     /translation="MPKVLSDAQVAFYRDKGYHFPLNVLSAEEVARFRRQLEDYEAAT
                     GGPIKGEMRHRSHVLFAWIDEMIRHPRVLDAVEDLLGPNILCWNTSFFIKEAHDPGFV
                     SWHQDATYWGLSSSDVATAWIAMSPANKVSGCMKFVAGTHLKQVAHEDTFERNNLLTR
                     GQEIAVKVDEAQAVHVELKPGQASLHHVLLFHGSEPNRSDERRIGLAIRYIPTHLKQA
                     VGSRDWATLVRGEDTYHNFEPAHVPKGDLEPEALAFHKMVSEEQVKVLYRGTGKTAYR
                     A"
     gene            complement(3944..>5426)
                     /locus_tag="VM910_25260"
     CDS             complement(3944..>5426)
                     /locus_tag="VM910_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVI65866.1"
                     /translation="TKAFRDACKAARAAGKPVIALKLGASEGGRAAAMAHTGALAGSI
                     ETFDAIATREGVIRVRGLDELIETTECFVHANLPKGDRLAAVTLSGGKRGLLIDAFHS
                     AGLNFAALSNDASEKLAKMLGPGSIVGNPLDAGFAAVVDPSVYMQSIKIMIDDPDTDI
                     VIIDSELPKAPHELRERNLRIVNEMAGRASKPVIYISAMSIGFTEFTKALRKSLPNVA
                     VMQGLDRAVEAIKSLIDYARLAKEVPDILSGSTAAARKVLEKTLKDAKGAALDEVASK
                     ALLKAYGIPISKEAIAQTAAEAVKIARKIGFPVVAKVVSPDILHKSDMGGVVLNLGNA
                     GEVKKAFNDITARVKKLKNKPKLEGILIAQQVKADLELVVGASLDAEMGPVVLFGTGG
                     IDIELMKDVALAGAPINAAEAKKLIARTKAGVKIKGYRGKPALHEPSVVKALVGLSNL
                     IADAGNRIASIDVNPFLINERTGVAVDALVVLNNAAANEAANH"
BASE COUNT         1036 a         1643 c         1673 g         1074 t
ORIGIN      
        1 gaaagattcc ctctgcgctc ggtacgccgc ggctgcccat tgcacccggt gcgccattca
       61 gtatcgcggg cgtcggcgtg accatccgcg gcgacggcaa gagcgcgggc atcgtccgca
      121 aggccgacct gatcgccacc ggcaagccgg gcacgctgca gatccgcctg gtcgatccag
      181 cgacgcaagg tgcgcgtgac gggctgtccg cctgcaccgg agattccggt gcaccggtat
      241 tcgaggagca gcaagccggg ccgatagtcg ttggcgtcgt aagctggtcg accgggccga
      301 acaatggcgc gggttgcggg ggattgaccg gcgtgacacc gctcacactc tatcgagact
      361 ggattctgca gaccgcccgg cagtggggtc tttcattgta aaaatcaaac actgtttgat
      421 ttttgattcg tatcaattgc tctgcgcaaa catgggttga ccgtggcagg cgattaagtc
      481 cagcctgccg atgccgatcc tccaaagcgc aagacaaaac aatgaacgtg tcagcagccc
      541 gctccatctc tgcctcgtct tcgcaagcga cagaacattt cgacgtgttg atcgtcggcg
      601 cgggcatctc cggcatcggc ggcgcctatc acctgacgac gcagcttcct ggaacgagct
      661 ttgtcgtttt ggaaagcaag gacagttttg gcggtacctg gctgacacat cgctatcccg
      721 gcatccgctc cgacagcgat ctctatacct tcggctatcg tttcaagccg tgggtcggcc
      781 cgccgatcgc aaccgccgag gaaattctcg cttacatggg tgaggtgatc gacgacaatg
      841 acttcgcagg gcacatccga tacaagcacc ggattttgaa agcgaactgg tcgagcgacg
      901 aaaatctctg gaccatcgaa gctgagagga ccgacaccgg tgagcaggtc ctgatcagcg
      961 cgaacttcct gtggatgtgc caaggctact ataggcattc ggaaggctac acgcccgaat
     1021 gggcgggtat gaaggacttc aaaggccgca tcattcatcc gcagacctgg ccggatgatc
     1081 tcgatctcaa gaacaagaag gtggtcgtga ttggttcggg tgcgacggcc gccactctga
     1141 tcccaaacat cgccgacgat tgcgcgcatg tcaccatgct tcagcgctcg ccgacctttt
     1201 tccgactcgg acgcaacgcc atcgaaatcg ccgaccagtt gcggcaactg caggtcaatg
     1261 aagactggat tcacgaaatc gtgcgccgca agattctgtt cgagcaagac gcgtttacgc
     1321 gccgctgcgt gagcgaaccg gaacaggtga cgaaggagtt gattggtcag atcagcggca
     1381 tactcggccc cgattacgac gtggcaacgc attttacgcc gcgctatcgg ccctggcgac
     1441 agcgcatcgc cttcgtgccg gattccgaca tgtttctcgg catcaagaac ggaaaggcat
     1501 cggtggtcac cgacgaaatc gagcgcttca cggagaaggg catcctgctg aaatccggca
     1561 agacgctgga agccgacatc atcgtcactg cgaccggctt caatctcagc gtgctcggcg
     1621 acattgcgtt tgccatcgac ggcaagccgc tggatttttc gaagaccatt acctatcggg
     1681 gcatgatgtt cacgggggtg ccgaacatgg cctgggtgtt cggctatttt cgcgcgagct
     1741 ggacgctgcg cacggacctg gtggcggatt tcgtctgtcg cctgctcaag cacatgaagg
     1801 acaagggcgc gaagagggtc gaggtggcgc tccgccccga ggaccacaac atgccgttat
     1861 tgtcctggat cgatccggaa aatttcaatc cgggctatat gatgcgcaac atgcacctgc
     1921 tgcccaagcg gggcgacaaa ccggagtggc agcacagcca ggattactgg accgagaagg
     1981 acgagtttgc ggcgatcgat ctcgacgacg cggcattcgt gtatagttga gctaggctac
     2041 tgccgcttct gctgcggcct gcgcggtggc tgcggcggcg ccggcgctac ggcggccggg
     2101 tctatcttgc gggtcatatt gttggcggcc tcgctcacat ccaatagcat gcgccggcca
     2161 tcttccagaa acgccgccga cttcttcttg taggtgtctg caagttcgcg cagggactgc
     2221 agcttctcga acaactcttc ggttttgccg tcggcaaggc cgggaacgac gccggtgatc
     2281 ttatcaatgg tgctatcgaa acctgcaaaa gcggcatcgg ccttgccgat aatgccgtcg
     2341 atcgcctcgc ccttgctctt caatgacgcc gtgtagctct cgaacgtgag cagcgcgtcc
     2401 ttgatcatgg cctggttttc gacaatgacg cggtcgacat ggtgcagcgt gtcggtcatg
     2461 gactgctgtt cgctccagtc ggcggtaagg acggggacgc catcccggtc cagcggcacc
     2521 ggaggcgcgc tcggcgcgcc gccgaccagc gcgatatcgg caaccccggt aaggccctgg
     2581 aactcgatgc cgacggtcgt gtccttgcgg atcggcgcgt tgttgtccag catgaccatg
     2641 gcgacgatcc tgtttgggct ctcgagcctg atcgatttga tcacgccgga aggaacgccg
     2701 tcgaaaatca ccgtaccgcc cttgcgcaac ccgctggcgg agccgttgaa aacgatgcga
     2761 agagggctct ggttgcgtac gccgtgaatt ttctggaaga cgagcagccc gacaaagccc
     2821 gcggcaatca ccgcaagcgt cgtcgtcccg atcatcagat tggtcacccg catggctcga
     2881 ttgtattgcc aaagcgggtc gaacgaaagg tggatggcgg ccggtgcggc accggatcga
     2941 cggtgcttga cagcgcgaga gggcagcgca acgcttgcct caagctccgc ggaacgggag
     3001 cgaaggagga acatcgtgcc caaagtgctg agcgacgccc aagtcgcctt ctaccgtgac
     3061 aagggctacc atttccccct caatgtgttg agcgcagaag aggtcgcgcg attccgccgc
     3121 cagctcgaag actacgaggc ggcgacgggc ggtccgatca agggcgagat gcgtcaccgc
     3181 agccacgtgc tgttcgcgtg gatcgacgag atgatccgcc atcccagggt tctcgatgcg
     3241 gtcgaggacc tgctggggcc gaacatcctg tgctggaaca ccagcttttt catcaaggag
     3301 gcgcacgatc ccggcttcgt gtcctggcat caggatgcga cctattgggg gctcagttcc
     3361 tccgatgtcg cgaccgcgtg gattgcgatg tcgccggcga ataaggtttc cggctgcatg
     3421 aagttcgttg ccggaacgca cctcaagcag gtggcgcacg aagacacgtt tgagcggaac
     3481 aatctcttga cccgtgggca ggagattgca gtcaaggtcg acgaagcgca ggccgtccat
     3541 gtcgaattga agccgggcca ggcctcgctg caccacgttc tgctgttcca cggatcggag
     3601 ccgaaccgct ccgacgagcg gcgcatcggg cttgccatcc gctacatccc gacgcacctc
     3661 aagcaggcgg tgggttcgcg cgattgggcg acgctggtgc gcggcgagga cacctaccac
     3721 aatttcgagc cggcccacgt gcccaagggc gacctcgagc ccgaggcgct ggcgtttcac
     3781 aagatggtat cggaagaaca ggtgaaggtg ctctatcgcg gcaccggcaa gactgcttat
     3841 cgggcctgat agatctcgac gggcgagagc gtaacccttt gctgtttgct tttgccctgc
     3901 cggccgttgc gatgcttggc ccagtcgggc tggcgtcgag gcgctaatgg ttagcggctt
     3961 cgttggcggc cgcgttgttc aggaccacca gcgcgtcgac cgcgactccc gtcctctcat
     4021 tgatcaggaa cgggttgacg tcgatcgaag caatgcggtt gccggcgtca gcaatcaggt
     4081 tggagaggcc aaccagcgcc ttcacgaccg acggctcgtg cagcgccggt ttgccgcgat
     4141 agcctttgat cttcacaccg gctttggtgc gggcgatcag ctttttcgcc tcggctgcgt
     4201 tgatcggcgc acccgccagc gccacgtcct tcatcaattc gatatcgatg ccgccggtcc
     4261 cgaacagcac cacgggaccc atctctgcat cgagggaagc accgaccaca agctcgagat
     4321 cggccttcac ctgctgcgcg atcaggatac cttcgagctt cggcttgttc ttcagcttct
     4381 tcacccgggc ggtgatgtcg ttgaatgcct tcttcacctc ccccgcattg ccgaggttca
     4441 gcaccacgcc gcccatatcg gatttgtgca ggatgtcagg gctcacgact tttgcgacca
     4501 ccggaaagcc gatcttcctg gcgatcttga ccgcctccgc cgcagtctgc gcgatcgctt
     4561 ccttcgagat cggaatgccg taagctttca gcagggcttt tgaggcgact tcatcaagcg
     4621 ccgcgccctt ggcgtccttc agcgtctttt ccagcacctt gcgggcggcc gccgtcgagc
     4681 ccgaaaggat gtcgggcacc tccttggcga gccgggcata gtcgatcagc gatttgatcg
     4741 cttcaaccgc ccggtccagc ccctgcatga ccgcgacatt cggcaacgat ttgcgcaagg
     4801 ccttggtgaa ctcggtgaag ccgatcgaca tcgcgctgat atagatcacg ggcttggagg
     4861 cgcgaccggc catttcgttg acgatgcgca agttgcgctc gcgcaactcg tgcggcgcct
     4921 tcggtaattc cgagtcgatg atgacgatat ccgtgtcggg gtcgtcgatc atgattttga
     4981 tcgactgcat gtagacggag ggatcgacga ctgcggcaaa tcctgcatcg agcggattgc
     5041 cgacgatcga gccgggcccg agcatttttg caagcttctc gctggcgtcg ttgctcagtg
     5101 ccgcaaagtt gagcccggcc gaatggaagg cgtcgatcaa caggccgcgc ttgccgcccg
     5161 acagcgtcac cgcggcgagc cggtcgccct ttggcagatt ggcgtggaca aagcattcgg
     5221 tggtttcgat cagttcgtcg agcccgcgca cgcggatcac gccttcgcgg gttgcgatgg
     5281 catcaaaggt ctcgatcgat ccggcaagcg cgccggtatg cgccatcgcc gcggcgcgac
     5341 cgccctcgga ggcgccaagc ttgagcgcaa tcacgggttt gccggcggcg cgtgcggcct
     5401 tgcaggcatc gcggaacgcc ttcgtg
//
LOCUS       DATLJT010001094         2592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3753031,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001094 DATLJT010000000
VERSION     DATLJT010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753031"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VM910_25265"
     CDS             <1..195
                     /locus_tag="VM910_25265"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687537.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HVI65867.1"
                     /translation="ICKNSPMSIRASKQAMRKGMEVPLEQAMTEQRDYPAVKAMAASQ
                     DYIEGPKAFAEKRPPKWLGR"
     gene            complement(245..631)
                     /locus_tag="VM910_25270"
     CDS             complement(245..631)
                     /locus_tag="VM910_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HVI65868.1"
                     /translation="MQDEPTPVELTKAVADFLRDDIAPMIAGHNAFKLRVAINMLNLV
                     IRQLTLAEGSDVAEAARLCRLLGIEGSLIELNRALADGIARGEVDLGTPGLAEHLWQT
                     TLDKLAVDQPNYASYRRELGGGKADA"
     gene            complement(711..1700)
                     /locus_tag="VM910_25275"
     CDS             complement(711..1700)
                     /locus_tag="VM910_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HVI65869.1"
                     /translation="MIEDALTRSVPSWYPGATGVAGAAKLSGGASQETWSFDIVHPQG
                     KVGAILRRAPKGHGAAPTRAAGLEAEAKLMQLAHDAGLPSPKVLHLLQPEDDLGTGFV
                     MQRVEGESIARKILRDELFAKARPLLARQLGRIAAGIHGLPRAQLPRLREMTAAKEIA
                     ELTREYRGFNWPRPVFELALRWLGRHDPGPPKEVTLVHGDFRNGNLIIGQDGVRAVLD
                     WELAHFGDPMEDLGWICVNSWRFGEIDKPVGGFGSREELFAGYEAAGGKIDAASVTFW
                     EVMGTLRWGVMCCGMMQGFRRSPDHSMERAMIGRRASETEIDLLRLLAPRGGP"
     gene            complement(1697..2491)
                     /locus_tag="VM910_25280"
     CDS             complement(1697..2491)
                     /locus_tag="VM910_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase"
                     /protein_id="HVI65870.1"
                     /translation="MQFKHCTLDVEGSVAVLKFDHQEVMNAVSMDMLGGLAEALDEVN
                     DRRAEVRCLVLTGAGRAFCTGANLQGRNQPQKPGRSNAGAALETGFHPFLRRLQNLHC
                     PLVTAVNGPAAGAGMSFALMGDMILVARSAYFLQAFRRIGLVPDCGSTWLLPRLIGKA
                     RSVELSLMGERLPAEKALEWGLINRVHDDAVLMEEAMKLARELASGPTVALSLIRKLY
                     WESPENSFEDQLNLEFQSQRIAGATEDFREGVTAFLEKRPAKFAGK"
BASE COUNT          460 a          883 c          784 g          465 t
ORIGIN      
        1 atctgcaaaa actcgccgat gtcgatccgt gcctccaagc aggcgatgcg caagggcatg
       61 gaggtcccgc tcgaacaggc gatgaccgaa cagcgcgact atccggccgt gaaggcgatg
      121 gcggcctcgc aggactacat cgaggggccg aaggcgttcg cggagaagcg accgccgaaa
      181 tggttggggc gataggcagc aggcgtttct cgtcatggcc gggcttgtcc cggccatccc
      241 ctttctaggc atcagctttg cctcccccca actcccgcct gtacgaggca tagttcggct
      301 gatcgaccgc gagcttgtcc aacgtggtct gccagagatg ctcggcaagc cccggcgtgc
      361 cgagatcgac ctcgccacgc gcgattccgt ccgcgagtgc gcggttgagt tcgatcagcg
      421 agccctcgat acccaaaagc cggcagagcc gtgcggcctc agccacgtca ctcccctccg
      481 caagcgtcaa ttgcctgata acaagattga gcatgttgat ggcgacgcga agcttgaacg
      541 cattgtggcc ggcaatcatc ggcgcgatgt cgtcgcgcag aaaatccgca accgccttgg
      601 tcagttcaac cggtgtcggt tcgtcctgca tctcaatctc caatcgggca tgatcttgcc
      661 tggaacccgg ttcccgcccc cgcatcaagc gcggggcagg cttttcggga tcatggccct
      721 cctcgcggcg ccagcagccg caacaagtcg atctcggtct cggaggcgcg gcggccgatc
      781 atggcgcgct ccatcgaatg gtcgggcgat cttcgaaaac cctgcatcat gccgcagcac
      841 atcacgcccc agcgcagcgt gcccatgacc tcccagaagg tcacgctcgc agcgtcgatc
      901 ttcccgccgg ccgcctcgta gcctgcgaac agctcctcgc gcgagccgaa gccgccaacg
      961 ggtttatcga tctcgccaaa gcgccaggag ttgacgcaga tccagccgag atcctccatg
     1021 gggtcgccga aatgcgcgag ttcccaatcg agcaccgcgc gcacgccgtc ctggccgatg
     1081 atgagattgc cattgcggaa atcgccatgc accagcgtca cttccttggg cggaccggga
     1141 tcgtgacggc cgagccagcg cagcgcgagc tcgaacaccg gccgcggcca gttgaagccg
     1201 cgatattccc gcgtcagctc cgcgatttcc ttcgccgccg tcatctcgcg caacctgggc
     1261 agttgcgccc gcggcaagcc gtgaatgccc gccgcgatcc ggccgagctg ccgcgccagc
     1321 agcgggcgcg ctttggcaaa cagttcgtcg cgcaaaatct tgcgcgcaat gctctcgcct
     1381 tcgacgcgtt gcatcacaaa gccggtgccg aggtcgtcct ctggctgcaa cagatgcagc
     1441 accttcggcg acggcaagcc ggcatcgtgc gcaagctgca tcagcttcgc ctcggcctcg
     1501 agccccgcgg cgcgcgtcgg ggccgctcca tggcccttcg gggcgcggcg caggatcgcg
     1561 ccgaccttgc cttgcggatg tacgatgtca aacgaccagg tctcctggct ggcgccgccc
     1621 gagagttttg cggcgcccgc aacgccggtc gcgcccggat accaggaagg gacggaacgc
     1681 gtgagcgcgt cctcgatcat ttgcctgcga attttgccgg ccgcttttca aggaacgcgg
     1741 tgacgccttc cctgaaatct tcggtcgcgc cggcgatgcg ctgcgactgg aattcgaggt
     1801 tgagctggtc ctcgaaggaa ttttccgggc tttcccagta gagcttccgg atcagcgaca
     1861 gcgcgaccgt tggcccgctt gcgagctcac gcgcaagctt catcgcttcc tccatcaaaa
     1921 ccgcatcgtc atggacgcga ttgatcaggc cccattccag tgccttctcc gccggcagcc
     1981 gctcgcccat cagggacaat tccaccgagc gcgccttgcc gatcaggcgc ggcaacagcc
     2041 aggtcgaacc gcaatcgggc acgagaccga tgcggcggaa ggcctgcagg aaataggccg
     2101 agcgcgcgac aaggatcatg tcacccatca gcgcaaagct cattccggcg cccgccgccg
     2161 gtccgttgac cgccgtgacc agcggacaat gcaggttctg cagccgccgc aggaacggat
     2221 ggaagccggt ttccagcgcc gctccggcgt tgctgcggcc cggcttctgc ggctggttgc
     2281 gaccctgcaa gttcgcgccg gtgcagaagg cgcggcctgc gccggtcaaa acaaggcagc
     2341 gcacctcggc cctgcggtcg ttgacctcgt ccagcgcttc ggcgagccca cccagcatgt
     2401 ccatggagac cgcattcatg acctcctgat gatcgaattt caggacggca accgaccctt
     2461 cgacatcgag cgtgcagtgc ttgaattgca tttttgtttc ctctttcaat tgttgcgcgc
     2521 gctacccggc gcgcggcgga ttttccagac catatttgat ttttgcggcg tcgttgtcca
     2581 tggttcaggc ag
//
LOCUS       DATLJT010001095         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2144009,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001095 DATLJT010000000
VERSION     DATLJT010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144009"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VM910_25285"
     CDS             complement(<1..534)
                     /locus_tag="VM910_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HVI65871.1"
                     /translation="MTRQLLLSLLCGCAFALPSFSRAHADDLPLRKAGLWELKIAKSG
                     SQLPDLTTQHCTDESTDRDMNNSVTPMAKQICSKQDVRKTATGYTVDAVCSVAGISMT
                     SHSEITGDLNSAYTVTTSSHSEGGPTSLREVTTKIDAKWLGACKEGQKPGDIVMPGGF
                     KINIKDAEKLKALLPKSN"
     gene            complement(600..890)
                     /locus_tag="VM910_25290"
     CDS             complement(600..890)
                     /locus_tag="VM910_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65872.1"
                     /translation="MRRIVAANLLILIAMLAGAPAKADWVPPFKGNDTGGIIAYSLAT
                     QADARQIAVNHCARYGKVVKFLAIDAQEGGYISFSCRWVPYGSQDRPLRVAY"
     gene            1069..1542
                     /locus_tag="VM910_25295"
     CDS             1069..1542
                     /locus_tag="VM910_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65873.1"
                     /translation="MRDLATSLKSLGLARLCLAASFALLAFGRPAHAANPLELNFWLS
                     GPQYEGRVAPCEKALGTIANQFQEKESTFWNSRLTITGYGNIHEVAFRPWQSDNIPRR
                     YCSGNAMTSDGRMHIVNFSIIEDGGFAGYDQGVEWCVTGLDRNWAYNPACKAAGP"
     gene            1648..2331
                     /locus_tag="VM910_25300"
     CDS             1648..2331
                     /locus_tag="VM910_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HVI65874.1"
                     /translation="MSRSIRIVLFALAFGVLLSAGGPALAQDQRQNAPGEFDFYVLSL
                     SWSPSFCEEAEERRSGGRSPAQCGGRPFSFVVHGLWPQYEHGFPEYCQRPSPRLDRNI
                     MRSMLDLMPAPGLIYNEWDKHGTCSGLSERAYFETIRKVRAVIKIPPDFLQLSEPKTV
                     APSDIEDAFIKVNPGLSNAAIAVTCNRSRLSEVRICLSKDLQFRDCDEIDRRACRRDQ
                     VTMPPVRGG"
     gene            2401..3276
                     /gene="rlmJ"
                     /locus_tag="VM910_25305"
     CDS             2401..3276
                     /gene="rlmJ"
                     /locus_tag="VM910_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HVI65875.1"
                     /translation="MNYRHAFHAAGFADVIKHIVLVRVLTYLHEKPAAFRVIDSHAGA
                     GLYDLTGEQAQRGGEWLTGIARILQARFSEATAALLKPYLDVVRAFNPQANMLAAYPG
                     SPLFARALLRPQDRLTACEVEPSARKALIDALRNDQQARVVDLDGWVALPAFVPPNER
                     RGLVLIDPPFEAKDEFARLEKGFLAAFNKWPTGIFLLWYPAKSRRLTDQLARQVARAA
                     ASTRPAAKCLRLEFSVAPQTQDAVLASCGLLIVNPPWTLQDELKIILPELEKPLGQGG
                     AGRFRLEKPTAGENP"
BASE COUNT          606 a         1084 c         1050 g          656 t
ORIGIN      
        1 gttcgacttc ggcagcagtg ccttcagctt ctcggcatcc ttgatgttga tcttgaagcc
       61 cccgggcatc acgatgtcgc cgggcttctg gccctccttg caggcaccca gccacttggc
      121 gtcgatcttg gtcgtgacct cgcgcaggct ggtggggccg ccttcgctat gcgaggaggt
      181 ggtgaccgta taggccgaat tgaggtcgcc ggtgatttcc gaatgtgaag tcatcgaaat
      241 gccggcgacg ctgcagaccg catcgacggt gtagccggtc gcggtcttcc gcacatcctg
      301 cttggaacag atctgcttgg ccatcggtgt gaccgagttg ttcatgtcgc ggtcggtgga
      361 ctcgtcggtg caatgctgcg tcgtcagatc gggaagctga gagccggact ttgcgatctt
      421 gagctcccag agtcccgcct tgcgcagcgg caggtcgtcc gcatgcgcgc gggagaaaga
      481 cggcagcgcg aacgcgcagc cgcaaagcag ggacaagaga agctgtcgcg tcatttgcgc
      541 gctccgcttt tgaggatcgt tctggcggaa agtcgtcggt cgttctggca gaaaaaaggt
      601 cagtaggcga cgcgcagggg ccgatcctgc gacccatagg gtacccagcg gcaggagaag
      661 gagatgtagc cgccctcctg ggcatcgatc gccaggaact tcacgacctt gccgtatctg
      721 gcgcagtgat tgaccgcgat ctggcgggca tccgcctggg tcgcaagcga ataggcgatg
      781 atgccgccgg tgtcgttacc cttgaaagga ggcacccaat ccgccttcgc cggcgcgcca
      841 gccagcatgg cgatgaggat caggagattc gcggccacaa ttcttcgcat caaagactcc
      901 atctcgcgcg cgacatctta cgaggcatct caccccaaga aaagaacgcc cggcgccgca
      961 ggtcgacttg cgttgcaagt gttgccgcat tgcacttgac ccgtcgagag cttcgcggca
     1021 cggtccgacg gggacggact cggttggatc caatccgacg gattgtacat gcgcgacctc
     1081 gctacatcct tgaaaagcct tggacttgcg cggctttgcc ttgccgcctc gttcgcgctc
     1141 ctggcgttcg gccggccggc gcatgccgcc aacccgctgg aactcaattt ctggctgtcc
     1201 ggcccgcaat atgaggggcg ggttgccccg tgcgaaaagg cgctcggcac catcgccaac
     1261 cagttccagg agaaggaaag caccttctgg aactcgcggc tcaccatcac cggttatggc
     1321 aatatccatg aggtcgcgtt ccggccctgg cagtccgaca acattccacg ccgctattgt
     1381 agtggtaacg ccatgaccag cgacggaagg atgcacatcg tcaacttctc gatcatcgag
     1441 gacggcgggt tcgccggcta cgatcagggc gtcgagtggt gcgtcaccgg gctcgatcgc
     1501 aactgggcgt ataatcctgc ctgcaaggcc gccggacctt agccaaccag ccgtccggcg
     1561 cgacctcgcc gcacattcca aaaatctgtt cttgaaatgt tccgaccgcc tgcttaggct
     1621 ccgcggccgt tgagtgaggg ggccgtcatg tccagatcga tccgtattgt cctgtttgcg
     1681 cttgcctttg gcgtgctcct cagtgccggc ggaccggccc tggcccagga ccagcgccag
     1741 aatgcgcccg gcgagttcga tttctacgtg ctgtcgctgt catggtcgcc gtccttctgc
     1801 gaagaggcgg aggaacgtcg cagcggcggg cgctccccgg cccaatgcgg cggccgaccg
     1861 ttctccttcg tggtgcatgg gctgtggccg caatatgagc acggctttcc cgaatattgt
     1921 cagcggccct cgccccggct cgatcgcaac atcatgaggt cgatgctcga cctgatgccg
     1981 gcgccgggcc tgatctacaa cgagtgggac aagcacggca cctgctccgg cctttcggag
     2041 cgcgcctatt tcgagaccat ccgaaaggtg cgcgccgtga tcaagatccc acccgatttc
     2101 ctgcaattgt ccgagccgaa gacggttgca ccctccgaca tcgaggacgc cttcatcaag
     2161 gtcaatccgg gcctgtcgaa cgcggcgatc gcggtgacct gcaaccgctc gcggctgtcc
     2221 gaggtgcgca tttgcctcag caaggatttg caattccgcg actgtgacga gatcgatcgc
     2281 cgcgcctgcc gccgcgatca ggtgaccatg ccgccggtcc gcggcggatg atcgattgct
     2341 ttgcttcgcc cgcaatgaca ggggttggtt ttctcgatct aacctcgtaa cccacggcgc
     2401 atgaattacc gacacgcctt tcatgcggcc ggctttgccg acgtcatcaa gcacatcgtg
     2461 ctcgtgcgcg ttcttaccta tctgcatgaa aagccggccg ctttccgtgt catcgacagc
     2521 catgccggcg ccggcctcta cgatctcacc ggcgagcagg cgcaacgcgg cggcgaatgg
     2581 ctcaccggga ttgcccgcat cctgcaggcc cggttttcgg aggcgaccgc ggcgctgctc
     2641 aagccctatc tcgacgtcgt cagagcgttc aatccgcaag ccaacatgct cgcggcctat
     2701 ccgggatcgc cgctgtttgc gcgggccttg ctgcgtccgc aggaccggct gaccgcctgc
     2761 gaggtcgaac cgtcagcgcg caaagcgttg atcgacgcgc tgaggaacga ccagcaggcc
     2821 cgcgtagtgg acctggacgg ctgggtggcc cttcccgcct tcgtcccgcc gaacgagcga
     2881 cgggggctcg tcctgatcga cccgccgttc gaagcgaaag acgaattcgc tcgtctggaa
     2941 aaagggtttc tggcggcctt caacaagtgg ccgaccggca ttttcctgct gtggtacccg
     3001 gccaagagcc gccgcttgac cgatcagctt gcgcggcagg tcgcccgggc ggccgcgtcc
     3061 acgaggcctg ccgcaaaatg cctgcggctg gagttcagcg tcgcgccgca aacccaggat
     3121 gcagtgctcg cctcctgcgg actcctgatc gtcaaccctc cgtggacgct gcaggacgaa
     3181 ctcaaaatca tcctgcccga actggagaag ccgctcggcc agggcggcgc cggccggttc
     3241 aggcttgaaa agcctacggc gggcgaaaat ccgtagtgaa tttgcggtga accgtattat
     3301 gctccgcaca ttgagactgg ctttacgttc cgcttccgcg aatggttgcg gcggagtgat
     3361 gaaggcctca aaaaaatcca ggcggaccgg cggcat
//
LOCUS       DATLJT010001096         2926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_994718,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001096 DATLJT010000000
VERSION     DATLJT010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994718"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VM910_25310"
     CDS             complement(<1..387)
                     /locus_tag="VM910_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HVI65876.1"
                     /translation="MSAILLAVTGLDPQPWEERMRTLAPQRDIRLWPQRVGDPADIAY
                     ACAWHAPRGLFARLPRLQAIFSLGAGVDHVIADPNLPDVPVVRIVDPDLTMRMTEYVV
                     LHVLMHHRRHRLYDTQQRERVWHEHAQ"
     gene            complement(429..1520)
                     /locus_tag="VM910_25315"
     CDS             complement(429..1520)
                     /locus_tag="VM910_25315"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI65877.1"
                     /translation="MSDRPARLTIRTSRIMAAASFVIGLAAQTASAQEMKIIRTEGHI
                     DALDPGIRLFVREKMQEGNTQFTNENVVLFLHGATAPSTCDFDLGYKDYSWADWMVKR
                     GYVVWMGDYRNYGYSTREKTMDEPAAKNQPVTRSYQALRDIEAMVNHIKRTRGVQKVT
                     LIGWSWGAMMAGYYASLHSENVHRLVLYAPLYNFNDHTNLGPGSALQNKRKPYEFNFA
                     LGAYRLASEAANTGRWNGEIPRENKDEYRDAAVPAAFWAECMATDPSSNTRNPPSLRA
                     PNGVLEDSFYQATGRPLWNASNIYVPTLVIAGAYDTWSFPEDREGLMRDLVHAPAKRS
                     VLIPDATHFVLFEKNRFQFFEEVLKFLKQ"
     gene            complement(1591..2151)
                     /locus_tag="VM910_25320"
     CDS             complement(1591..2151)
                     /locus_tag="VM910_25320"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVI65878.1"
                     /translation="MAAAPDLFWRTVPGPLSHEDGRAQQRSQPRLEKIKQGEADMADT
                     LARLGELPQFNGSAVAMGFCYGGPYAILGPKRLGYAAGISCHGTQMLDYLREIEDVSE
                     PVCILWGDRDHRAPDDVLSAYRALAAKKNNVELHIFPGVQHGYMMQGMPKAYDARARE
                     FSMTRAVAILDGFRSSKVRKAVREAS"
     gene            complement(2350..>2926)
                     /locus_tag="VM910_25325"
     CDS             complement(2350..>2926)
                     /locus_tag="VM910_25325"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI65879.1"
                     /translation="ADVIIAQGTEGGGHVGWQTTMTLVPMVVDAVAPIPVLAAGGIAD
                     GRGLAAAIMLGADGVLLGTRFLASRESSLHPNFKQAILESNGHDTLLSEIPDVAAGLV
                     WPGAMSRSRRNRFIERWAGREWALRQHQAGAKAALQAARKSGDVEEAVLSMGQDAGLI
                     HDIAPAAEIVSRIAQDAERILTDRLTQFIRR"
BASE COUNT          520 a          950 c          892 g          564 t
ORIGIN      
        1 ctgcgcatgc tcgtgccaca cgcgctcgcg ctgctgggta tcgtagaggc gatggcgacg
       61 gtgatgcatc aacacgtgca gcaccacata ctccgtcatg cgcatggtga gatcgggatc
      121 gacgatgcgc acgaccggca cgtcgggcag attcggatcg gcgatgacgt gatcgacgcc
      181 ggcaccgagc gaaaagatcg cctgcagacg cggcaaacgg gcaaacagcc cgcgcggcgc
      241 gtgccaggcg caggcgtagg cgatgtcggc gggatcgccg acgcgctgcg gccacagccg
      301 aatgtcgcgc tgcggcgcca gcgtgcgcat gcgctcctcc cagggctgcg gatcgagtcc
      361 ggtcacggcg aggaggatgg cggacatgcg gacggcgtat cgcgttcggg ccgttcctcc
      421 tgtgcgcgct attgctttag aaacttgagc acttcctcaa agaactgaaa gcgattcttc
      481 tcgaacaaga cgaaatgcgt cgcgtcggga atcaacacgc tgcgtttcgc cggtgcatgc
      541 accagatcgc gcatcagtcc ttcacggtcc tccggaaacg accatgtgtc ataagcgccg
      601 gcgatgacca gggtagggac gtaaatgttg gatgcgttcc acagcggccg gccggtggcc
      661 tgatagaacg agtcctccag taccccattc ggagcccgca agctcggtgg attacgcgtg
      721 ttgctgctcg gatcggtcgc catgcactcg gcccagaacg cggcggggac cgcagcgtcc
      781 cgatattcgt ccttgttctc gcgcgggatc tcaccgttcc atcgtcccgt gttcgccgct
      841 tccgaagcga gccgatacgc tcctagcgca aagttgaatt cgtacggctt gcgcttgttc
      901 tgcaaggcgc tgccgggacc gaggttggtg tggtcgttga agttgtagag cggcgcatag
      961 agcaccaacc tgtgcacatt ctcgctgtgg agcgaagcat agtagccggc catcatggcg
     1021 ccccaggacc agccgatcag cgtcaccttc tgcacgccac gggtgcgctt gatgtgattc
     1081 accatcgctt cgatgtcgcg caacgcctga tacgagcggg tcaccggctg atttttcgca
     1141 gccggttcgt ccatcgtctt ctcgcgcgtc gaatagccgt aattgcggta gtcgcccatc
     1201 catacgacat agccgcgctt caccatccaa tcggcccacg aataatcctt gtatccgaga
     1261 tcgaaatcgc aggtcgacgg ggcggtcgca ccatgcagaa agagaacgac gttttcgttc
     1321 gtgaactgcg tgttcccctc ctgcattttt tcccgcacga atagtcggat gcccggatcg
     1381 agcgcgtcga tgtgcccctc agttctgatg attttcatct cctgcgcgct ggctgtttga
     1441 gctgctaacc cgatcacgaa ggaggccgca gccatgatcc tcgacgtcct gatcgtaagg
     1501 cgtgccggtc tgtcgctcat gcttattcct cccctccggt catgtcagaa tgacgctgtt
     1561 gctcggggcc ccggcgccgc atgcggacgg tcacgaagcc tcgcgtacgg ccttccgcac
     1621 cttgcttgag cgaaaaccgt cgagaatcgc caccgcgcgc gtcatggaaa actcgcgcgc
     1681 tcgcgcgtcg taggccttgg gcattccctg catcatgtag ccgtgctgca cgcccggaaa
     1741 gatatgcaac tccacattgt tcttttttgc tgccagggcg cgataagcgc tcaacacgtc
     1801 gtccggagcg cggtggtccc gatcgcccca taggatgcaa acaggttcgg agacgtcctc
     1861 gatttcgcgc aggtaatcga gcatctgggt gccgtggcag gaaatgccgg cagcgtagcc
     1921 gaggcgtttc ggcccgagga tggcgtaggg gcccccatag cagaagccca tcgccaccgc
     1981 gctcccgttg aattgcggca gttcgccgag ccgcgccagc gtgtcggcca tatcggcctc
     2041 gccttgtttg atcttctcga gccgcggctg cgaacgctgc tgggcgcgcc catcctcatg
     2101 ggagagcggg cctggaaccg tgcgccagaa caggtccggc gcggccgcga tgacgtcatg
     2161 tgcggcgaac tatcggcgat cgcgatgatg tcggcatcga cgccatgaac cgcgcaagca
     2221 agcacaacgg ccggaacctg gctgccggca ttgggaacgt tgagatagca atcgaagtcg
     2281 ccgcccgcgc tcgagcggac cttgatccac tgccctgaca tcgcatgcct cctgttcgct
     2341 gcggcaatcc taccttctga tgaactgcgt aagccggtcg gtcaatatgc gttcggcgtc
     2401 ttgcgcaatg cggctgacga tctcggcagc aggcgcgata tcgtgaatga gcccggcatc
     2461 ttgccccatc gacaggacgg cttcctcgac gtcgccgctc ttgcgcgccg cttgcaatgc
     2521 tgccttcgct cccgcttggt gctgacgcaa cgcccattcc cgtcccgccc accgctcgat
     2581 gaaccggttg cgccgcgagc gtgacatcgc cccgggccac accagcccag ccgcgacatc
     2641 cggtatttcg gagagcagcg tgtcgtgccc gttgctctcg agaatggcct gcttgaaatt
     2701 gggatgcagc gacgattccc ggctggccag gaatctggtt ccgagcagca cgccgtcggc
     2761 gcctagcatg atcgccgccg ccagtccgcg cccgtcggca attccacccg cggcgagcac
     2821 cggaatggga gcgacggcgt ccaccaccat gggcacgagc gtcatggtcg tttgccagcc
     2881 gacatgcccc ccgccctccg tgccttgcgc aatgatcacg tcggcc
//
LOCUS       DATLJT010001097         3083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_76847,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001097 DATLJT010000000
VERSION     DATLJT010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76847"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1174..2241
                     /gene="pqqE"
                     /locus_tag="VM910_25330"
     CDS             1174..2241
                     /gene="pqqE"
                     /locus_tag="VM910_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HVI65880.1"
                     /translation="MVHKPLGLLAELTHRCPLGCPYCSNPLAFDAREDELNTATWGRV
                     FREAAGLGVLQVHLSGGEPGARRDLVDITAGAHAAGLYTNLITSAVGITGQTLGALAE
                     AGLDHVQISIQDCDAASADHIAGYDGAFARKRALAAEVVRHKLPLTVNAVVHRANIDR
                     IGGMVDLALALGASRVEIAHVQYHGWALKNRAALMPSREQVERATAAVEELRARHHGR
                     IVIDAVVPDYYARYPKPCVGGWGRRSLNVTPAGKVLPCHAAESITGLEFWNVRERSLA
                     DIWANSPAFNAFRGRAWMKQPCASCPRRELDFGGCRCQAFALTGDATATDPVCHLAPD
                     HARVAELAAVRSDAPYVYRRM"
     gene            2344..2496
                     /locus_tag="VM910_25335"
     CDS             2344..2496
                     /locus_tag="VM910_25335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65881.1"
                     /translation="MPIRFSPQRGFLLAATLASTPARATECRARSHFEKKLQVSIEKH
                     YTKKPD"
     gene            2620..2942
                     /locus_tag="VM910_25340"
                     /pseudo
     CDS             2620..2942
                     /locus_tag="VM910_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171709.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
BASE COUNT          586 a          990 c          958 g          549 t
ORIGIN      
        1 gtgggagagg atgacaggag ccccccgaaa tgcgctagcg cataggcgct aatcgcggta
       61 gcgaggccag cggcgctgac ctcgacgaac ttcacgaaga aacgcgagag gtaggaattc
      121 tcgcccgaca tggaaatccc ccaccgaagg aaccgtgggg aattccaacg gccacctcag
      181 ggcgaattca gggcgccagc tgcgaggaaa ggtgacgata ctgtggaaaa agttaacgtt
      241 ttgccacctt ctgccgggtg acgggatcag gcccaacctc caacaggtcg aggcagtggg
      301 ctgttcagac gcgctcggtg ccgcgaacga gcgtgccggt gatacgggca cggtatcttt
      361 ggcctagccg ggtggcgctg gctgttcctg ttggaaggcc tgccggcggc cgttctcggc
      421 gtcatttgcc ttttctacct cgatgacggc cccgcgcatg ccaattggct cagcgacgaa
      481 gagaaacggg agattgcatc acggcttgag cgcgatcgcg cactggagca aaccgccgct
      541 acgcagcgcg gcattctgaa ccagcttggc agccgaaacg tgctttcatt gtcccaccgg
      601 ccgacctgtg aggagtccga cgatgaattg gcatcatcgc gaaccaacgc tcgacgagat
      661 cttgtccgac tccattgtca gggccgtgat ggaggtcgac accctataag gaagactatg
      721 tgcgcgacac ggtaacggcg atcaaggata tggacatcga ctacatcatt ccactgcact
      781 gcactggtga gccgttctac gagagggcta aggcagcggc atcctagcgg ctaccatgag
      841 agtggcttcg gtcgaaaacc ccgcagcgca ggctccgctc gcggccaagc tttatttcga
      901 ctgccacgac gcccttaggg ccgaacgcaa gaggcgctac gaggtctagc gttcctgatc
      961 gcgcgggatg ttgttgatcc gaagcgcgtc tttcgctgcc aatcgcgcat ggcggcccgg
     1021 cttgcctcat attcggctct tacctaggcc ccacgaattt gattggccac gaatggttat
     1081 tatgtactgg tggctgtgcc gttacccatt gaaaatgaca aaggccggac agagcattgg
     1141 atcaacggtc ccgtggcgtt atcgagttcc acaatagtgc acaagccgct cggcctgctc
     1201 gccgaactga cccaccgctg tccgctcggc tgcccctatt gctcgaaccc gctcgcgttc
     1261 gatgcgagag aggacgaact caacaccgcg acttggggac gcgtcttccg cgaggcggca
     1321 ggccttggcg tcctccaggt gcatttgtcg ggcggcgagc ccggcgcgcg gcgggacctt
     1381 gtcgacatca cggccggcgc ccatgccgcc ggtctctaca ccaatctcat cacctccgcg
     1441 gtcgggatca cgggccagac cctcggcgcg ctcgccgagg ccgggctcga ccatgtgcag
     1501 atctccatcc aagactgcga cgccgcatcc gccgaccaca tcgccggcta cgacggggcg
     1561 tttgccagga agcgggcgct cgcggccgag gtcgttcggc ataagctgcc gctcaccgtg
     1621 aacgccgtgg tgcaccgcgc caacatcgac aggatcggcg gcatggtcga tctcgcgctc
     1681 gcgctcggcg ccagccgggt cgagattgcg cacgtgcagt accacggctg ggcgctgaag
     1741 aaccgcgccg cgctgatgcc gagccgcgag caggtcgagc gtgccactgc ggcggtcgag
     1801 gaattgcgcg cgcgtcacca cggccgcatc gtcatcgacg ccgtggtgcc cgactactat
     1861 gcccgctatc caaagccttg cgtcggcggc tggggtcggc gctcgctcaa cgtgacgccg
     1921 gcgggcaagg tgctaccctg ccacgccgcg gagtcgatta ccggccttga attctggaac
     1981 gtgcgcgagc gttcgctcgc cgatatctgg gcaaactcgc cggccttcaa cgccttccgc
     2041 ggcagggcat ggatgaagca gccgtgcgcg agctgcccgc ggcgcgagct ggatttcggc
     2101 ggctgccgct gccaagcctt cgcgctcacc ggcgacgcca ccgctaccga cccggtgtgt
     2161 cacctcgctc ccgatcatgc ccgggttgcc gaactcgcgg ccgtgcgcag cgacgctccc
     2221 tacgtctacc gccgcatgtg agccggcatc aggcgtcgac ccccgatctc tgattccgat
     2281 tgcgcttggg gtgcgacctg tcggacactg cggccgagga aatccacaat cagggaggat
     2341 cgcatgccca tccgcttctc gccgcagcgg ggcttcctcc ttgcggcgac gctcgcgagc
     2401 acgcccgcgc gcgccaccga atgccgtgcc cgttcccatt tcgaaaaaaa acttcaagta
     2461 tcgattgaaa agcattacac aaagaagcct gactgaaatc gcaagagcgc ggccgctcgg
     2521 aaggcaccgt tcgaatcttc ggagcgaccg cttcacgaga actgaccccc gtcctgcggc
     2581 gcggccatta cgtcgttacc aaccaacttt gggaggacta tccatcgcgg agatttcaat
     2641 tcaccggcag tggacaatgg cgtgaagccc ggctcggcga acttcgcggg ggggaacgct
     2701 cgtgtgccaa tgcgcgagca atccgtcgaa gtgagcatta cgtccccagt gcgcctataa
     2761 tcacgtgtgc ggatgcacca aatgctggca gcctgaaggc gcacttttct cgcaagtcgc
     2821 cgttgtgccg cgcgataagc ttccgtgtgt ccaagaatgc ggaaaagctc aaggtcgtgg
     2881 atgccaatgc ggccattcaa ctgcacgcct gcagggattg cgacatgcac atgtatggcc
     2941 gagagtggat cgttcttggc cagacgctaa ctctagccga gcttgcctct agctcgatgt
     3001 tcgacgtgcc gcgcgcgagc ggccggcggt tgcgttgccg cggcgaacgg ccggcatgct
     3061 caagccgacc ccgagaaagc ggc
//
LOCUS       DATLJT010001098         3299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_537453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001098 DATLJT010000000
VERSION     DATLJT010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VM910_25345"
     CDS             <1..502
                     /locus_tag="VM910_25345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65882.1"
                     /translation="GNVGTRNLWRYVGAALVICLIAAIPLAAVLAALALFDLLPAAGR
                     TPPDVIAVIALAYVVSVALMLRLSLLLPARATGNPSLSLKDAWRHTHGNLWRLFFGIV
                     ACTVPPVVLIKIIFIGLTVVPVGSGIYLAQWALAGAISLCCWLLVWPIWVGFLSHAYR
                     YLVRTA"
     gene            complement(511..1311)
                     /locus_tag="VM910_25350"
     CDS             complement(511..1311)
                     /locus_tag="VM910_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI65883.1"
                     /translation="MYVRLWRAAPAVLFAAAMMAASPAHSAELLDEIAPTGKLRVAIA
                     ISTAGGAFWSNKTDSGGYTGVPVDLGKEMAAQLGVPVEYVAYDNSGQITDAVPKKTWD
                     VAFLPKDPVREAQMSFGPIYDVADATYIVKAGSTITNFQTLDQPGVKVAAEYNTTTMR
                     GARAHLKNAKVIEYQTFDEIFELLKNGEIDAFALSRDQLNKMATKIPGTKVLDETFKK
                     TVVAVAVPLNHPLALAFVTKFMTDARTNGILRKAYDNNGLKDAPIRTE"
     gene            1547..2740
                     /locus_tag="VM910_25355"
     CDS             1547..2740
                     /locus_tag="VM910_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI65884.1"
                     /translation="MKPAHPSCPEPVDRRLFGPILRAVIPVLASFALIGCEQNTFVPP
                     PPPKVDVATPVQRSITRYLEATGNTAAVKSVDLVARVQGFLQSIDYKDGDFVKEGTTL
                     FTIEPETYKLKLEQAQAAEAGAQATLKQAEYDFRRQTDLVARQAVSQATLDTSTANRD
                     NAQANLLQAQANTRIAEVNYGYTKVTAPFDGVVSAHLVSVGELVGAASPTQLASIVAL
                     DPIYVNFNVNERDVIRIRAEAMRKGLTPSDLKQVPVEVGLQTETGYPHEGKLDYVSPT
                     INQSTGTLAVRGLIPNPNRVLLPGYFVRVRVPVEQQNDALLVPDTSLGSDQGGRYLLV
                     VNKDNVVEQRKVTTGPVEGELRVIDDGLKADDRVVIAGLLRVIPGEKVDPQVQKIEQA
                     PQSAK"
     gene            2751..>3299
                     /locus_tag="VM910_25360"
     CDS             2751..>3299
                     /locus_tag="VM910_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI65885.1"
                     /translation="MISKFFIERPVLSNVIAILMILIGGVALFELAIAQYPDVVPPTV
                     QVTTRYPGASAKTVVDTVALPIEQQVNGVEDMLYMQSYSAADGTYSLTVTFKIGTDLN
                     FAQVLVQNRVSSALSQLPQAVQNQGVTVQKKSTSILLFVTLTSPDARFDSLYLSNYAT
                     INLKDELARLPGVGNVTVFGAGQ"
BASE COUNT          609 a         1042 c          974 g          674 t
ORIGIN      
        1 cggcaatgtc ggcacacgca atctttggcg ctatgtcggc gcagctctcg tgatctgcct
       61 gatcgcagcc atcccgcttg ctgccgtgct cgccgcactc gcgctgttcg atttgctgcc
      121 ggccgccggc cggacgccgc cggacgtgat cgccgtcatc gcgctcgctt atgtcgtcag
      181 cgtcgcgttg atgctgcgcc tcagcctcct gcttccggcg cgcgcgaccg gtaaccccag
      241 cctctcccta aaggacgcgt ggcgtcacac acatgggaat ctctggagac tctttttcgg
      301 gatcgtggcc tgcacggtac cgcctgtcgt tctgatcaag atcatcttca tcggcctgac
      361 cgtcgtcccg gtcggaagcg ggatctatct cgcgcaatgg gcgctcgccg gcgcgatctc
      421 gctctgctgc tggctgttgg tctggccgat ctgggtgggc tttctctccc acgcctaccg
      481 atacctcgtc cggacggcgt gatcgctctc tcattccgtc cggatcggtg catccttcag
      541 accgttattg tcataggcct tgcgcaggat cccattcgtg cgggcgtccg tcatgaattt
      601 cgtgacaaag gcgagcgcca gcggatggtt gagcggaacg gcgacggcga ccacggtctt
      661 cttgaatgtc tcgtccagca ccttcgtgcc cgggattttc gtcgccatct tgttgagctg
      721 gtcgcgcgac agcgcgaaag cgtcgatctc gccgttcttc aacagttcga atatctcatc
      781 gaaggtttga tactcgatta cttttgcatt cttcagatgc gctcgcgcac cgcgcatggt
      841 ggtcgtatta tattcggctg cgactttgac gccgggctga tcgagtgtct gaaaattggt
      901 gatcgttgat cccgccttga cgatgtaggt cgcgtccgcg acgtcataga tcgggccgaa
      961 tgacatctgc gcctcacgca ccgggtcttt cggcagaaag gcaacatccc acgtcttctt
     1021 ggggaccgca tccgtgatct gtccggaatt atcgtatgcg acgtattcga cgggcacgcc
     1081 aagctgtgcc gccatctcct ttcccagatc gacgggcacc ccggtatagc ccccggaatc
     1141 cgttttgttg ctccagaatg cgccgcccgc ggtgctgatc gcgatcgcca cccgcaattt
     1201 gccggtcggc gcgatctcgt ccagcagctc cgcagaatgt gcagggctgg ccgccatcat
     1261 tgcagcagca aacagaacgg ccggtgcggc gcgccacaat cgaacataca ttgcatcccc
     1321 tccccgtcac ggcagacatc ggcggcgcca ttctggctta tggttcgcaa atttgaaaag
     1381 ccggatttcc gatcttgcga taaaataatc atctccatga aaaggcgttt cgcgccgcaa
     1441 ttgcgcgctt ccatggtgtt atcgcttgcg ggttccactg atggtggatt gttgctatgg
     1501 agaaccttcg agcctgctct caacgcgaag cgaatgggac actgcaatga agcctgcgca
     1561 tccgtcatgt ccggagccgg tcgaccgcag gctttttggt ccgattctcc gcgccgtcat
     1621 tccggttctc gcgtcgtttg cgctgattgg ctgcgagcag aacaccttcg tgccgccgcc
     1681 gccgcccaag gtcgatgtgg cgacccccgt gcagcggtcg atcacgcgat atctcgaggc
     1741 taccggcaac accgcggcgg tcaaatcggt tgatctcgtc gcgcgggtgc agggcttcct
     1801 gcaatcgatc gactacaagg acggcgactt cgtcaaggaa ggcacaacgc tcttcacgat
     1861 cgagcccgaa acctacaagc tgaagctcga gcaggcgcaa gccgcagaag ctggcgcgca
     1921 ggcgacgctg aagcaggccg agtacgactt caggcggcag accgatctgg tcgcgcgcca
     1981 ggcggtgtcg caggcaacgt tggatacctc caccgccaac cgcgacaacg cgcaggccaa
     2041 cctcctgcaa gcgcaggcca ataccaggat cgccgaagtc aattacggct acaccaaagt
     2101 aaccgccccg ttcgatggcg tcgtcagcgc gcatctggtc tcggtcggtg aactggttgg
     2161 agcggcatcg ccgacgcagc ttgcgagcat cgtggcgctt gatccgatct acgtgaattt
     2221 caacgtcaac gagcgggacg tgatccggat tcgcgcagaa gcgatgcgca aggggctgac
     2281 ccccagcgac ctcaagcagg tgcctgtcga agtcgggttg cagaccgaaa ccggctaccc
     2341 gcatgagggc aagctcgatt acgtgtcgcc gaccatcaat caatcgaccg gaaccctggc
     2401 ggtgcgcggc ctcattccca atccgaaccg ggtgttgctg cccggatact ttgtccgcgt
     2461 tcgtgtcccc gtcgagcagc agaacgacgc gctgctcgtt cccgacacat cgctcggcag
     2521 cgatcagggc ggacgctatt tgctggtggt caacaaggac aatgtggtcg agcagcgcaa
     2581 ggtgacgacc gggccggtcg aaggtgaact gcgggtgatc gatgacgggc tgaaggcgga
     2641 tgatcgcgtg gtgatcgcgg gtctgctgcg ggttatcccc ggcgagaagg tcgacccgca
     2701 ggtacagaaa atcgagcaag cgccgcaatc tgccaaataa cagggcctgc atgatctcca
     2761 aattcttcat cgagcggccg gttctctcca acgtcatcgc catcctgatg atcctgatcg
     2821 gcggcgtggc gctgttcgag cttgcgattg cccaatatcc ggatgtggtg ccgccgaccg
     2881 tgcaggtcac cacgcgctat cccggcgcca gcgccaagac cgtggtcgat accgtcgcgc
     2941 tgccgatcga gcagcaggtc aacggtgtcg aggacatgct gtacatgcag tcctatagcg
     3001 ccgccgacgg cacctatagc ctcaccgtga cgttcaagat cggcaccgac ctcaacttcg
     3061 cgcaggtgct agtgcagaac cgcgtgtcca gcgcgctgtc gcagttgccg caggcggtgc
     3121 agaaccaggg cgtcaccgtc cagaagaaat cgacatcgat cctcttgttc gtgacgctga
     3181 cctcgcccga cgcgagattc gacagcctct atttgagcaa ctacgccacc atcaatctca
     3241 aggacgagct ggcgcggctg ccgggcgtcg gcaacgtcac ggtattcggc gccgggcaa
//
LOCUS       DATLJT010001099         6775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3064261,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001099 DATLJT010000000
VERSION     DATLJT010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064261"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1321
                     /locus_tag="VM910_25365"
     CDS             107..1321
                     /locus_tag="VM910_25365"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HVI65886.1"
                     /translation="MAKIKVTNPVVELDGDEMTRIIWKYIKDKLIHPFLDIQLQYFDL
                     GIEYRDRTNDQVTVDAANAIKKVGVGVKCATITPDEARVKEFGLKEMWKSPNGTIRNI
                     LGGVIFREPIICRNVPRLVPGWTKPIIIGRHAYGDQYRATDIKFPGKGTLSLKFVGED
                     GTVIEREVFKAPGAGVAMEMYNLDDSIIDFARASLNYGLMRGYPVYLSTKNTILKVYD
                     GRFKDIFQDVYDREFKKEFEAKKLTYEHRLIDDMVASALKWSGGYVWACKNYDGDVQS
                     DTVAQGYGSLGLMTSVLLTPDGKTVEAEAAHGTVTRHYREHQKGKETSTNSVASIFAW
                     TRGLSHRAKLDNNPELAKFADTLEKVCVQTVEDGYMTKDLALLVGADQRWLSTTGFLD
                     KVAENLNKAMAT"
     gene            1450..1956
                     /locus_tag="VM910_25370"
     CDS             1450..1956
                     /locus_tag="VM910_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HVI65887.1"
                     /translation="MTRTLAIALLLSGPLTGAAVAQSLLPLALAALPDETMALKVHAV
                     GMQLYECKPGTDGKLAWTFTAPQATLTVDGKVVGHHGAGPSWELDDGSGITGKAVANA
                     PGTNANDIPWLKLEVASHTGSSGKLSDVTTVQRINTIGGVLKDSCDRQGAGRGMPYAA
                     DYVFWKKS"
     gene            1964..2248
                     /locus_tag="VM910_25375"
     CDS             1964..2248
                     /locus_tag="VM910_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HVI65888.1"
                     /translation="MLFMVIERFKDRDPIPVYKRVRDGGIKFPDGLKYVGSWIEPNFD
                     RCFQLMETDDARLLQEWILNCQGLGMTFEIVPVVPSKETREVVARYLDRT"
     gene            complement(2255..3484)
                     /locus_tag="VM910_25380"
     CDS             complement(2255..3484)
                     /locus_tag="VM910_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HVI65889.1"
                     /translation="MATVPRRRRLNDPDLRDRLYELLEHDRLPGAHGLRLVRLIILVI
                     VLDVGTTVLASVPDYHARFGGLFTAIKIFAVSVFALEYLARLWSVPGHSPRKLSPWRD
                     RFAFAVSALGIIDLMAFLPATIALVLGAHSTLLALVALPFFKLVRYSPAMRSLLAAIH
                     AERRTLIGCLVILTGAVLLFASLLYAIERDVQPDKFGTMPEAIWWAIVTLGTVGYGDV
                     VPVTPLGKFVSVFAIVSSFAMVALPVAIIATAFAEEVRRRDFVVTWSMLARVPLFAHL
                     TAGEIADIMRLLRARTIEPGEILVRRGEPATSMYFITAGEVEIQLPNQNVRLSDGTFF
                     GEIALLHRTKRSGTVTATRSTRLLVLDAQDFHALIERVPALAAHVKKTAKARLADSGD
                     LASAELALAEHEGEPQH"
     gene            complement(3638..6313)
                     /gene="alaS"
                     /locus_tag="VM910_25385"
     CDS             complement(3638..6313)
                     /gene="alaS"
                     /locus_tag="VM910_25385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HVI65890.1"
                     /translation="MSSVNDIRSSFLNFFARNGHEIVPSSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGVEKRPYVRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDRAIELAWKLVTKEFGLPKEKLWATVYIDDDEAYGLWKKISGLPDSRIVRIAGSDNF
                     WQMGDTGPCGPCSEIFYDHGDKIWGGPPGSPEAEGDRYIEIWNLVFMQFEQLEGGVRN
                     PLPKPSIDTGAGLERVAAVLQGQHDNYDIDLFVSLIRAISELTGANPHGQHKASLRVI
                     ADHLRASSFLIADGVLPSNEGRGYVLRRIMRRAMRHAQLLGAREPLMYRLVWALVREM
                     GQAYPELVRAESLIEETLRLEETRFRKTLERGLAILDEKSASLKKGDMFDGDTAFTLY
                     DTYGFPLDLTQDALKSRGIGVDVAAFTDAMDRQREKARAAWAGSGDTATESIWYPLRE
                     KLGATEFLGYETETAEGAVAALVKDGQEVGSLKAGESGAIVLNQTPFYAESGGQIGDT
                     GVMTGEGVKFRVIDTQKKAGDLFVHQGTMEQGTLKVGAALQLDVDHARRSSIRRNHSA
                     THLLHEALRQVLGDHIAQRGSLVAPDRLRFDFAHPKPITAEELARVEDIANDVVLEND
                     EVTTRLMALDDAREAGARALFGEKYGDEVRVVSMGKGLREHGQNAFGWSVELCGGTHV
                     KRTGDIGLISVTSDSAVSSGVRRIEALTGQHARKHANDTMALARTAAHELRTSLDDVP
                     GRIASLMEERKRLERELSDARKKLAMGGGAAANGTAAGGVREVGDVKLMARAVEGVEM
                     KDLKSLADDGKKQLGSGVVAIVGVTDDGKAGVVVGVTPDLTSRYNAVDLVRVASVALG
                     GKGGGGRPDMAQAGGPDGAKADAALSAIEKAMAGG"
     regulatory      6513..6601
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT         1311 a         2184 c         2098 g         1182 t
ORIGIN      
        1 tcgcgtgctc ggcttccgct tgaagatttg cagtgctatg acgcgctatt ccccgcccac
       61 cccgctcttg aagaaagcgc gctgttccca agaggccttc cccatcatgg caaaaatcaa
      121 ggtgaccaac cccgtcgtcg aactcgatgg cgacgagatg acccggatca tctggaaata
      181 catcaaggac aagctgattc atccgttcct ggatattcag ctccagtatt tcgacctcgg
      241 gatcgaatac cgcgacagga ccaacgatca ggtcacggtc gatgccgcca atgccatcaa
      301 gaaagtcggc gtcggcgtca aatgcgcaac catcaccccg gacgaggctc gggtgaaaga
      361 gttcgggctg aaggagatgt ggaagtcgcc taacggcacc atccgcaaca tcctgggcgg
      421 cgtgatcttc cgcgagccga tcatctgcag gaacgtgccg cggctggtgc cgggctggac
      481 caagccgatc atcatcggcc gccacgccta tggcgaccaa taccgggcga ccgatataaa
      541 atttcccggc aagggaacgc tgtcgctcaa attcgtcggc gaggacggca ccgtcatcga
      601 gagagaggtg ttcaaggcgc ccggcgcagg cgtcgcgatg gagatgtaca atctcgacga
      661 ctccatcatc gacttcgccc gcgcctcgtt gaattacgga ttgatgcgcg gttacccggt
      721 ttatctctcg accaagaaca ccatcctgaa ggtctatgac ggccgcttca aggacatctt
      781 ccaggacgtc tacgatcgcg agttcaagaa ggaattcgag gccaagaagc tcacctacga
      841 acatcgcctg atcgacgaca tggtggcctc ggcgctgaaa tggtccggcg gctatgtctg
      901 ggcctgcaag aactacgacg gcgacgtgca gtccgacacg gtggcgcagg gttacggctc
      961 gctcgggttg atgacgtccg tgctgctcac tcccgacggc aagaccgtgg aagcggaggc
     1021 cgcccacggc acggtgacgc ggcactatcg tgagcatcag aagggcaagg aaacctcgac
     1081 caattcggtc gcttccatct tcgcctggac gcggggcctc agtcaccgcg ccaagctcga
     1141 caacaatccg gagcttgcga agtttgccga tacgctggag aaggtctgcg tgcagaccgt
     1201 cgaggacggc tacatgacca aggatctcgc gctgctcgtc ggcgccgacc aacgctggct
     1261 gtcgaccacg ggcttcctcg acaaggtcgc ggaaaacctc aacaaggcga tggcgaccta
     1321 gtcggaactc gtcgttgcga gcgaagcgag gcaatccagg cctgaatgag cttgaagttt
     1381 gccggatctg tttcggagct catgctcctc gcaatgacgc aaattcgagg catccaggga
     1441 gtaacggcaa tgacgcggac gcttgcaatc gctctcctgc tgtcgggtcc gcttacgggc
     1501 gcggcggtgg cgcaatcgct actgccgctt gccctcgcgg cgctgccgga cgagacgatg
     1561 gcgctcaagg tgcatgcagt gggcatgcag ctctacgaat gcaaacccgg cacggacggc
     1621 aagctcgcct ggacgttcac cgcgccgcag gcgacgttga cggtcgacgg caaggtggtg
     1681 gggcaccatg gcgcaggtcc ctcctgggag ctggacgacg gcagcggtat caccggcaaa
     1741 gccgtcgcca acgcccccgg cacgaatgcg aatgatatcc cctggctgaa gctcgaggtc
     1801 gcctcccaca caggcagcag cggcaagctc tccgatgtca ccaccgtgca gcggatcaac
     1861 acgatcggcg gggttttgaa ggacagttgt gaccggcaag gcgccggccg cggcatgccc
     1921 tatgccgccg actatgtgtt ctggaagaag agctgaaggg ctcatgctgt tcatggtcat
     1981 cgaacgtttc aaggaccgcg atccgatccc ggtctacaaa cgcgttcgag acggaggcat
     2041 caagttcccg gacgggctga aatatgtcgg aagctggatc gagccgaatt tcgatcgctg
     2101 tttccagctc atggagacgg acgacgcgcg cctgttgcag gaatggatcc tgaactgcca
     2161 ggggctcggc atgaccttcg agatcgtgcc ggtggtgccg agcaaggaaa cgcgcgaggt
     2221 cgtggcgcgc tatctggacc gcacgtaatc gcagtcagtg ttgcggctcg ccttcgtgct
     2281 cggcgagcgc aagttctgcc gacgcaaggt cgcctgaatc cgcaagccgc gcttttgcgg
     2341 ttttcttcac atgcgcagcc agcgccggaa cccgttcgat cagggcatga aaatcctggg
     2401 catccagcac caggagcctc gtgctgcgcg tggcggtgac cgtgcccgag cgcttggtcc
     2461 ggtgcagcag cgcgatctcg ccaaagaacg tgccgtcgga aagccgcacg ttctgattcg
     2521 gcaactggat ctctacctcg cctgcggtga tgaaatacat cgaggtcgca ggctcccctc
     2581 gccgcacaag gatttcgccg ggctcgatgg tccgcgcccg caacagccgc atgatgtcgg
     2641 cgatctcgcc agccgtaaga tgcgcaaaca gcgggactcg cgccagcatg ctccaggtga
     2701 cgacgaaatc gcgtcgcctg acctcctcgg cgaaagcggt ggcgatgatc gccaccggca
     2761 gcgcgaccat ggcgaagctg gagacgatgg cgaacaccga gacgaacttg ccgagcggcg
     2821 tcaccggaac gacatcgcca tagccgaccg tcccgagcgt cacgatcgcc caccatatcg
     2881 cctccggcat cgtgccgaac ttgtctggct ggacatcgcg ctcgatggcg tagagcaacg
     2941 aggcaaacaa caataccgcg cccgtgagga tgacaaggca gccgatcaga gtgcgccgct
     3001 cggcgtgaat cgccgcaagc agcgaccgca tcgcggggga atagcgcacc agcttgaaga
     3061 acggcagcgc gaccagcgcc agcagcgtcg aatgcgcgcc caaaacaagt gcgatcgtgg
     3121 cgggcaagaa cgccatcagg tcgatgatgc caagcgcgga gacggcgaac gcaaaccggt
     3181 cccgccacgg cgaaagcttg cgcggcgaat gcccgggtac gctccacagc cgggccagat
     3241 attccagcgc aaacacactc acggcaaaga tcttgatcgc cgtgaaaaga ccgccgaacc
     3301 gcgcatgata gtccggcacc gaggcaagca ccgtcgtccc cacgtcaagg acgatgacga
     3361 ggatgatcag ccggaccagg cgcaagccat gtgccccggg cagacgatcg tgctcgagca
     3421 gttcgtagag gcgatcccgc aagtccgggt cgttcagtcg tcgcctgcgc ggaacggtcg
     3481 ccaccttgcc tgcctctcga ctttcactgc agcttgcctt gcgccctgcc cggccctcca
     3541 gggatttcgg gactgagact ccatcgcgca tcctgcacga accgcggcaa cgccgcttga
     3601 tctccatcaa aagcggtgcg gcgcaaacgc gaaccggtca gccgccggcc atggcctttt
     3661 cgatcgccga cagcgcggca tcggctttgg cgccgtccgg accgccggcc tgcgccatgt
     3721 cgggccgtcc gccgccgccc ttgccgccca gcgccaccga ggcgacgcgc accaggtcca
     3781 ccgcattgta acgcgaggtg aggtcgggcg tgaccccgac cacgacgccg gccttgccgt
     3841 catcggtcac accgacgatg gcgacgacgc cggagccgag ttgcttcttg ccgtcgtcgg
     3901 caaggctctt gagatccttc atctcgacgc cttcgaccgc gcgcgccatc agtttgacat
     3961 cgccgacttc gcgtacgccg cccgccgccg tgccattcgc cgcagcgccg ccgcccattg
     4021 cgagcttctt tcgcgcgtcc gacagctcgc gttcgagtct cttgcgttct tccatcaggg
     4081 aagcgatgcg cccgggcaca tcgtcgagcg aggtgcgcag ctcatgcgcc gcagtcctgg
     4141 cgagcgccat ggtgtcgttg gcatgcttgc gcgcgtgctg acccgtcagc gcctcgatgc
     4201 ggcgcacgcc ggacgaaacg gcgctgtccg atgtcaccga gatcaaccca atatcaccgg
     4261 tgcgcttcac atgcgtgccg ccgcaaagct cgaccgacca gccgaacgca ttctgcccgt
     4321 gctcgcgcag ccccttgccc atcgagacga cgcggacctc gtcgccatat ttttcgccga
     4381 atagcgcacg tgcaccggct tcgcgggcgt catcaagcgc catcaggcga gtggtgacct
     4441 catcattttc cagcaccacg tcgttggcga tgtcttcgac gcgggcgagc tcttccgccg
     4501 tgatcggctt gggatgcgca aaatcgaagc gcagccgatc gggcgccacc agcgagccgc
     4561 gctgggcgat gtgatccccg agcacctgtc gcagcgcttc atgcaaaaga tgcgtcgcgg
     4621 agtgattgcg ccggattgac gagcggcggg cgtggtcgac atccaactgc agcgcggcgc
     4681 cgactttgag cgtgccctgc tccatggtgc cctgatgcac gaagagatcg ccggccttct
     4741 tctgcgtgtc gataacgcga aacttgacgc cctcgcccgt catcacgccg gtatcgccga
     4801 tttggccgcc cgattccgcg tagaacggcg tctggttcag cacgattgcg ccgctttcgc
     4861 cggccttcag gcttccgacc tcctgaccgt ccttcacgag cgcagcgacc gcgccctccg
     4921 cggtctcggt ctcgtagccc aggaactcgg tcgccccgag cttctcgcgc agcggatacc
     4981 agatgctttc cgtcgcggtg tcgccgcttc ccgcccaggc cgcgcgcgcc ttctcgcgct
     5041 ggcggtccat cgcatcggtg aaggccgcaa catcgacccc gatgcctcgc gatttcagcg
     5101 cgtcctgggt gagatcgagc ggaaagccgt aggtgtcgta cagagtaaag gcggtgtcgc
     5161 cgtcgaacat gtcgcctttc ttcaacgacg cgctcttctc atcgaggatg gcgagcccgc
     5221 gctcgagcgt cttgcggaag cgggtttcct cgagcctgag cgtctcttcg atcaggctct
     5281 cggcacgtac cagctccgga taggcctgcc ccatctcccg caccagcgcc cagaccaggc
     5341 gatacatcaa cggctcgcgc gcgccgagca actgcgcatg gcgcatggca cggcgcatga
     5401 tccgccgcag cacatagccg cggccctcgt tggaaggcag cacgccgtca gcgatcagga
     5461 acgacgacgc gcgcaaatga tccgcgatca cgcgcagcga ggccttgtgc tggccgtgcg
     5521 gattggcgcc ggtcaattcc gagatcgcgc ggatcagcga gacgaacagg tcgatgtcgt
     5581 aattgtcgtg ctggccctgc aggacggcgg cgacgcgctc gaggcctgcg ccggtgtcga
     5641 tcgagggttt gggcagcggg ttgcgcaccc cgccttcgag ttgctcgaac tgcatgaaca
     5701 ccaggttcca gatctcgata tagcggtcgc cctccgcttc gggcgagccg ggcggcccgc
     5761 cccagatctt gtcgccgtgg tcatagaaga tttccgagca cgggccgcac gggccggtgt
     5821 cgcccatctg ccagaaattg tcggagcctg cgatgcggac gattcgcgag tccggaaggc
     5881 cggaaatctt cttccacaat ccgtaggctt catcgtcgtc gatatagacg gtagcccaca
     5941 gcttctcctt gggcagcccg aattccttcg tgaccagttt ccaggccagt tcgatcgcgc
     6001 gatccttgaa atagtcgccg aaggaaaaat tgccgagcat ctcgaagaag gtgtgatgcc
     6061 tggcggtgta gccgacattg tcgaggtcgt tatgcttgcc gcctgcgcga acgcatttct
     6121 gcgaggtggt ggcgcgcaca taagggcgct tctcgacgcc ggtgaagacg ttcttgaact
     6181 gcaccatgcc ggcattggtg aacatcaaag tcgggtcgtt gcgcggcacg agcggcgagg
     6241 acggcacgat ctcgtggccg ttccgggcaa agaagttcag gaagctcgac ctgatgtcgt
     6301 tgacgctgct catgttcatc caaccggagg ccgggccgga ccgcgaaagg gcccttttct
     6361 ccggcccaag gcttttagac aagggcaacc gcgctgtcca gaaactgtgc aggcaaatca
     6421 ggggcttgct gcgagcgcat aaagcccgtt gatagctgcg gcggcgcgtt gacagtctgg
     6481 tagaggtcgt gtttccctcc tatctgttca agacagtcac gtcgggagag atcggctcaa
     6541 gtgccggcgc cgaaggagca accgccccgg aaactctcag gcagaaggac cgcgtgacga
     6601 ggacagcatc tggaaagaga cgccgagggc tttagccgag ggcgtccgcc gacgggataa
     6661 tactctcagg cacagcgaca gatggggctt tgcgaggctt tcagggaatg gtccccggga
     6721 gcctgcgagg gccctatcga tgcttgcgcg acacgacctt cccctcaaac gcacg
//
LOCUS       DATLJT010001100         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1915792,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001100 DATLJT010000000
VERSION     DATLJT010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915792"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VM910_25390"
     CDS             complement(<1..571)
                     /locus_tag="VM910_25390"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65891.1"
                     /translation="MIAPSGEQIEIAVEDQQAVVVEVGGGLRSYSAGGRELVDGYGAD
                     EMSSSGRGQVLIPWPNRLQDGCYEFDGRRHQLPLNEPEHHNAIHGLVRWAAWTTAERE
                     PHRAVMEHVLNPQPGYPFSLRISIEYALTDSGLRVRTTARNVGTDPCPYGSGAHPYLT
                     LGTATIDRLILRVPGRTVLRSDERGLPIGT"
     gene            complement(712..915)
                     /locus_tag="VM910_25395"
     CDS             complement(712..915)
                     /locus_tag="VM910_25395"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVI65892.1"
                     /translation="MQRARDLMERLLRVASPLGLYAEEFDPETRRHLGNFPQAFSHRA
                     LIEAAPRMIVPTLAHFSPSLCPT"
     gene            complement(1071..1325)
                     /locus_tag="VM910_25400"
     CDS             complement(1071..1325)
                     /locus_tag="VM910_25400"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI65893.1"
                     /translation="MFRYTKIAGVAEAIEAAGAMLLYLPPYSPDLNPIEPAFSKVKAH
                     VRKAAEHTIPRLLRRIGRIVTGFSAQECRNFFRRAGYVRT"
     gene            1486..1842
                     /locus_tag="VM910_25405"
     CDS             1486..1842
                     /locus_tag="VM910_25405"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVI65894.1"
                     /translation="MPRVADQPLSTQAATSQPHHAGGRACLAGWLGGLAPIADFNPLL
                     QMASGVYLTFFGSFVFGTPGFPLSDVPLQSIANDVAAGRYKAKPSRIFRFEEIREAHR
                     VMEANEARGKMVVVLG"
     gene            1940..2128
                     /locus_tag="VM910_25410"
     CDS             1940..2128
                     /locus_tag="VM910_25410"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HVI65895.1"
                     /translation="MAVLRRATHERLSSFSDGVFAVLITVLVLELRPPELPTFEALLS
                     LWPTWLSYAVSYLFIAIV"
     gene            2455..>3187
                     /locus_tag="VM910_25415"
     CDS             2455..>3187
                     /locus_tag="VM910_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI65896.1"
                     /translation="MVAVMNVSRQRVVIVGGGFGGLSAARALARVAVDVTLIDRHNYH
                     LFQPLLYQVATAALAPNTIAAPIRAILRAQKNATVLMETVTGIDMAGRAVLIGDRHVA
                     YDFLIIATGARHAYFGHDDWEKFAPGIKSLADALSIRQQILSAFEAAETSNDPSERRR
                     LLNFVLVGAGPTGVELAGAIAELAKASLVHDFCSIDPSQARVILIEAGPRLLPAFSER
                     SSAAAKRALERLGVEVRVGKPVTACD"
BASE COUNT          526 a          994 c         1005 g          662 t
ORIGIN      
        1 ccgtgccgat cggaaggccg cgctcgtcgg accgcaggac ggttcgcccg ggtacgcgca
       61 aaatcaaacg atcgatagtt gcggtcccaa gcgtaaggta cggatgagcg ccgcttccgt
      121 agggacacgg gtccgtgccg acgttcctgg ccgttgtccg cacccggaga ccgctgtccg
      181 tcagcgcata ttcgatgcta atcctgagcg agaagggata gcccggttgt ggatttagga
      241 cgtgttccat cacggcccgg tggggttcgc gctcggcggt ggtccaggcg gcccagcgga
      301 cgaggccgtg gattgcattg tggtgctccg gctcgttcaa tgggagctgg tggcgccgcc
      361 cgtcgaactc gtagcagcca tcttgtagcc ggttcggcca cgggatcagc acctgacccc
      421 ggcccgagga gctcatctcg tcggctccgt acccgtcgac aagctcccgg ccgccagcgg
      481 agtatgagcg gagtccacca ccaacctcga cgaccacagc ttgctgatct tcgaccgcga
      541 tctcgatctg ttcgccggat ggcgcgatca tcgctcgcct cccgtcgggc tggccgcgcg
      601 agcagggtcg ccgatgctga gcgcgtgggc cgtcgcggtc aacacgggct tcacaaagat
      661 gcaagagtcc ttgttgcaga gtgcccccgc cggcgcccgt cctgatgatg atcatgtcgg
      721 gcacaaagat ggtgagaaat gcgcgagcgt ggggacgatc atgcgcggcg cggcctcgat
      781 aagcgcacgg tgggagaacg cctgcgggaa attccctagg tgcctccgtg tctcagggtc
      841 gaactcctcg gcatagaggc cgaggggtga tgcgacccgc agtagtcgct ccatcaggtc
      901 acgcgctcgc tgcatctctc ccagagagcc cgtcgtcggt ctcgtcggtg cggtagtcgg
      961 gacgcggggg gcaaaaaatt ctcacgcgcc cgggcgcggg gcgcggcggc ggtgctattt
     1021 ttcaaatcgt ccattcagtc acgctgacca attctagagc ggatttccga tcacgttcgg
     1081 acatagcccg cgcgccgaaa gaagttcctg cattcttgtg cgctgaaacc ggtgacaatg
     1141 cgaccgatcc ttcgcaagag gcgcggaatc gtatgttccg ccgctttgcg cacatgggcc
     1201 ttcactttgc tgaaggccgg ctcgatcgga ttgaggtctg gcgagtacgg cggaagataa
     1261 agcagcatcg cgcccgccgc ttcgatcgcc tccgcgacac cggcgatctt tgtgtaccgg
     1321 aagattgtcc atgatcacga tatcgctgcg cttgagtgtg ggaacgagac attgctttac
     1381 gtaagcgaga aacatcggtc cattgatggc cccttcgaac acgaaggggg cgaccatcgc
     1441 gcgataccgc agcccggcca cgaacgtgat tgttttccac tgtccgtgcc gcgcgtagcc
     1501 gatcagccgc tctccacgca ggcagcgacc tcgcagccgc accatgctgg tggtcgcgct
     1561 tgtcttgctg gttggcttgg cggcctcgcc ccgatcgccg atttcaaccc gcttctgcaa
     1621 atggcgagtg gcgtctatct gacgttcttc gggagcttcg tgttcggaac cccgggcttt
     1681 ccgctttcgg acgtcccgct gcagagtatc gccaatgacg ttgccgccgg ccgttacaag
     1741 gcgaagccat cccgcatctt taggttcgag gagatccgtg aggcgcatcg cgtgatggag
     1801 gccaacgagg cccgcggcaa gatggtcgtc gttctcggat gatcgacgcc cctgtcgcgc
     1861 ggtgtggcgc cggtcccgga ggaggaaccc gtcgccctga tgccaattac cggctcgacc
     1921 tcactgggca ggaaatgaca tggcggtgct ccgtagagcg acgcacgagc ggctgagctc
     1981 attctcagac ggggtgttcg ctgtccttat aacagtgctg gttttggagc tgcggccacc
     2041 tgaacttccc acttttgagg cgctcctgtc gctttggcca acatggctaa gctacgcggt
     2101 aagctaccta ttcatcgcca ttgtctgaac caatcaccat tatctcctgc gctatgccgc
     2161 ggagacgacc ctcgcttatt gtggttcaat ttcgcgcatc tgttttccat gtcgctgctt
     2221 ccgctctctt ccgcttggat ggctgtgagc acattggcgc cgcaaacggt tgccttctac
     2281 gcggcggtgt ggttagtttc ctgccgcggc ttgtcgggcc catttcatag gctgcctcgg
     2341 cgtcggcttc tttttcggag gcgccaggga agcgatggcc gcgctcctag gtcaggtcgt
     2401 tttcggcgcc atcctcggcg tgatcgcgcg cctgccttcc cggcacgtcc aagcatggtc
     2461 gcggtgatga atgtctcaag gcaacgcgtc gtgatcgtgg gtggtggctt cggcgggctg
     2521 tcggcggcac gcgccctcgc tcgggttgcg gtcgatgtca ctctgatcga ccgccacaac
     2581 taccacctgt ttcagcctct gctctatcag gttgccacgg cggcgctggc gcccaacacc
     2641 attgcagccc caattcgcgc aatcctgcgc gcgcagaaaa atgcgaccgt gctcatggag
     2701 accgtgaccg ggatcgatat ggcaggccgc gccgttttga ttggcgaccg ccacgtggcc
     2761 tacgacttcc tgatcatcgc gacgggggcg cgccacgcgt attttggcca tgacgattgg
     2821 gagaagttcg cacctggcat caagtcgctg gcggatgccc tatccattcg ccaacaaatc
     2881 ctctccgcct tcgaggccgc cgaaacctcg aacgatccga gcgaacgccg ccggctgctt
     2941 aatttcgtgc tggtcggtgc cggtccgacc ggcgtcgagc ttgccggcgc catcgcggag
     3001 cttgcgaagg cgtcgctggt ccatgatttt tgcagcatcg atccctccca ggcgcgagtc
     3061 atcctcatcg aggctgggcc gaggctgctt ccggctttct ccgagagatc gtctgctgcc
     3121 gcgaaacgtg cgctcgaaag gctcggcgtg gaggtgcggg tggggaagcc ggtcactgcg
     3181 tgtgacg
//
LOCUS       DATLJT010001101         8271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_995030,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001101 DATLJT010000000
VERSION     DATLJT010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995030"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..300
                     /locus_tag="VM910_25420"
     CDS             151..300
                     /locus_tag="VM910_25420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65897.1"
                     /translation="MKRRTFITLLGGAAVWPFTARAQQPASPVVGFFHLQYSASDATP
                     TRPPA"
     gene            361..1338
                     /locus_tag="VM910_25425"
     CDS             361..1338
                     /locus_tag="VM910_25425"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65898.1"
                     /translation="MTRREFIRIVGGAASTWPMAARAQQGKLPTIGFLGSGTAATQSQ
                     WTAAFVRRLRELGWSEGRSVAIEYRWAEGRNERAAEIAAEFVRLKVEVIVTYGTQSIF
                     AAKQATTIIPIVFALPGDPVATGLVASLARPGGNLTGLSSQATDLGAKRVEILREAVP
                     GLRRLAIMGNVGNPANVLDMAKVQAAARSLGLDAVIFKIRRAEDIAPTFEALTGRMDA
                     LYVPADALINTNRIRINTLALGARLPTMHGSRDYVEAGGLMSYGVNFADQFRRAADYV
                     DRILRGAKPSDIPVEQPTKFDLVINLTTAKALGLTIPESFLLRADEVIE"
     gene            1335..2066
                     /locus_tag="VM910_25430"
     CDS             1335..2066
                     /locus_tag="VM910_25430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65899.1"
                     /translation="MNRRTFIALVGGAAAAWPLTARAQQPAMPVVGFLDTRSPDGMTD
                     RLRAFREGLKDSGYVERENVAIEYRWAENQLDRLSELAAELVRRQVAVIAATSSYPAL
                     AAKAATTTTPIVFLVGEDPVKLGLVASLARPGGNLTGINLFSVELTAKRLGLLREMVP
                     GAARIAVLVNPANTTTTETTLRDVQSAARAMGLQIQVLCRLRKDQSRAARRHLRRGHP
                     VLELPACPAGTTGGISSTSCGPCTA"
     gene            1993..2292
                     /locus_tag="VM910_25435"
     CDS             1993..2292
                     /locus_tag="VM910_25435"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI65900.1"
                     /translation="MNSRHVQLAQLAAFHRLPAAHALRDYAEAGGLMSYGSDIVEGYR
                     QVGVYTGRILKGAKPADLPVLQASKFELVINTRTARMLRLTVPPTLLARADEVIE"
     gene            2289..2552
                     /locus_tag="VM910_25440"
     CDS             2289..2552
                     /locus_tag="VM910_25440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65901.1"
                     /translation="MKRRAFITLLGGAAAWPLTARALQSAKPLIGFLYSLPPYAIADR
                     LRAFRQGLKETGYVEGQNVAIEYRSRVKLTGCRFWWRTFFAGR"
     gene            2991..3131
                     /locus_tag="VM910_25445"
     CDS             2991..3131
                     /locus_tag="VM910_25445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65902.1"
                     /translation="MAEQAAKSAERNLRKVHRVEKEFQELLSQLRAEFDAAIAKERKP
                     AP"
     gene            3364..3543
                     /locus_tag="VM910_25450"
     CDS             3364..3543
                     /locus_tag="VM910_25450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65903.1"
                     /translation="MTEMATAAGLTTRQIASAIQQIEQKRLASVARRRDGFDSRARQY
                     RIVRLKPGDKMVTLL"
     gene            3806..4135
                     /locus_tag="VM910_25455"
     CDS             3806..4135
                     /locus_tag="VM910_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65904.1"
                     /translation="MDRYERARRLIEEEELRVSRAARRPADGLLPPSQRKDARPPAPE
                     PDAAPFTKEELIDGIADCVAALLAQRDERIAELGAKLDAVLQILGTGKSGDIIDLPKN
                     FWRRDAA"
     gene            4521..4763
                     /locus_tag="VM910_25460"
     CDS             4521..4763
                     /locus_tag="VM910_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65905.1"
                     /translation="MAEKLVVDVGFDAAHGHYVAHHPELKPFTALSLFGLRKKIEAAL
                     LPETVDIKLVLNRSARLERDRRRRSGEQGRDRAWPR"
     gene            complement(4896..5117)
                     /locus_tag="VM910_25465"
     CDS             complement(4896..5117)
                     /locus_tag="VM910_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65906.1"
                     /translation="MAKLTTNLSAQDRVILFCAATGIDHAAVGIVAKAMQSMAVRGFI
                     VHNRETGVYALTDSGRATLTAILENAGLK"
     gene            complement(6186..6440)
                     /locus_tag="VM910_25470"
     CDS             complement(6186..6440)
                     /locus_tag="VM910_25470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65907.1"
                     /translation="MLRPAYLRTKLSKALPTKDGGTLRTIEDARAYMLKLSKQRETRA
                     QWQRAAELLLAEADVAGLTRQIELALFYDAKLDVTTIDTA"
     gene            complement(7074..7268)
                     /locus_tag="VM910_25475"
     CDS             complement(7074..7268)
                     /locus_tag="VM910_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65908.1"
                     /translation="MSKERRVYKPRIVGDKVMMPADLKRLHKYMREVEHIDHISDEMR
                     AVVESEWPELAYKLPPKDLH"
     gene            7639..7875
                     /locus_tag="VM910_25480"
     CDS             7639..7875
                     /locus_tag="VM910_25480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65909.1"
                     /translation="MKLTIHDGELARYLVHCKHAGKPKRVFVPYESDVLVFATSPEDA
                     KIAAAEKLQDKWLRHRGVHTEWRATDVRLDWSTR"
BASE COUNT         1637 a         2511 c         2512 g         1611 t
ORIGIN      
        1 cgatcccgtt cgaatgatga ggactcggtt cgcggacccc atcagggcca gcggtccacc
       61 cggactgcac caacaggccg gacacatgac tgcaagcgac ctctgcgctg cacgccaaat
      121 gatctcttgc caggcggggg ccatccacac atgaagcgtc gcacattcat cacactgctc
      181 ggcggcgccg cggtctggcc gttcacggca cgcgcacagc aaccggcatc gccggtggtc
      241 ggtttttttc atctgcaata ttcggcctct gacgccactc caacgcgccc gccggcgtga
      301 acttcatgtg gtatcttcca aattacattc gcgtagtcct gagtgttggg gaggagctgt
      361 gtgacgcgac gggaattcat ccggattgtt ggcggggcgg cgagcacatg gccgatggcg
      421 gcgcgcgcgc agcagggcaa gctaccgacc attgggtttc taggctctgg cacggctgca
      481 acacagagcc aatggaccgc cgcctttgtg cggcggctgc gtgaactggg ttggagcgag
      541 gggcggagtg tcgcgatcga gtatcgctgg gcagagggtc gcaatgagcg cgccgccgag
      601 atcgcagccg agttcgtccg gctcaaggtc gaggtgattg tgacatatgg gacccaatcg
      661 atcttcgcgg caaagcaggc gaccacgata atcccaatcg tctttgcgct gccgggcgat
      721 ccggttgcca ccggcctcgt agcgagtctg gcgcgaccgg gcggcaacct caccggcctg
      781 tcgagccaag cgacagatct tggagccaaa cgtgtcgaaa ttctacgcga ggctgtgccc
      841 ggtctgcgcc gattggcaat tatgggcaat gtcggcaatc ccgctaacgt tctggacatg
      901 gctaaagttc aagcggcagc gcgcagcctt ggcctcgatg ccgttatctt caaaatccgg
      961 cgtgccgaag atatcgcgcc cacatttgag gcgctcacgg gccgcatgga cgcgctttat
     1021 gtccctgccg atgcgctcat aaacaccaac cgcattcgca ttaatacgtt ggcactcggc
     1081 gctcgactgc cgacgatgca cggctctcgt gattacgttg aagcgggtgg tttgatgtcc
     1141 tatggagtaa actttgctga ccagttccgg cgcgctgctg attatgttga taggattctg
     1201 cgcggagcga agccaagcga tatcccagtc gagcagccga ccaagttcga tctcgtcatc
     1261 aacctcacga ctgccaaagc gcttggcctg acgattccag agtcgttcct gctgcgcgcc
     1321 gacgaggtga tcgagtgaac cggcgcacct tcatcgcgct cgtcggcggc gcggcagccg
     1381 cgtggccgct cacggcgcgc gcgcagcagc cggcgatgcc agtggtcgga tttctcgaca
     1441 ccagatcgcc tgacgggatg acggaccgtt tgcgcgcatt tcgcgagggc ctgaaggata
     1501 gcggctacgt cgagcgcgag aacgtggcga tcgaataccg ctgggccgag aaccaactcg
     1561 atcgactgtc ggagctggcg gccgaattgg ttcgccgaca ggtcgccgtg atcgccgcga
     1621 ccagcagcta tccggcgttg gctgccaagg cagcaaccac gacgaccccc atcgtgttcc
     1681 tcgtcggcga agacccggtc aagttgggtc tggtcgccag ccttgcccgg ccgggcggca
     1741 acctgacagg gatcaatctt ttcagtgttg agttgaccgc aaagcgtttg ggactcttgc
     1801 gtgagatggt gcccggagcg gctcgtatcg ccgtgctcgt caatccggcc aatactacga
     1861 ccacggagac cacgttgaga gacgtgcagt cggctgcccg cgctatggga ttgcaaatcc
     1921 aggtcctatg ccgccttcga aaggatcagt cgcgagcggc ccgacgccat cttcgtcggg
     1981 gccaccccgt tcttgaactc ccggcatgtc cagctggcac aactggcggc atttcatcga
     2041 cttcctgcgg cccatgcact gcgtgattac gccgaagccg gtgggttgat gagctacgga
     2101 tcggacattg tcgaagggta tcgtcaggtt ggggtctaca ccggccgtat tctcaagggc
     2161 gccaagcctg cggatctacc agtcctgcag gcgagcaaat tcgagctggt catcaacacc
     2221 cggaccgccc ggatgcttcg cctcaccgtg ccgccgacgc tgctcgcccg cgccgacgag
     2281 gtgatcgagt gaagcggcgc gccttcatca cgctgctcgg cggcgctgcc gcttggccgc
     2341 tcacggcgcg cgcgctgcag tcggcgaagc cgttgattgg gttcctctac agcctaccgc
     2401 catacgcgat cgcggaccga ctgcgcgcat ttcgccaagg cctgaaagag acaggctatg
     2461 tcgagggaca gaacgtcgcg atcgagtacc gctcgagggt caaactgaca ggttgccgct
     2521 tctggtggcg gaccttcttc gccggcaggt agccgtgatc atcggcaata ccccttcggc
     2581 gctcgcggcc aaggccgcac ccacgacggt gccgatcgtc tttgtgaccg ggggcgaccc
     2641 ggtcagggat ggcctcgtcg ccaacctcaa ccggcccggc ggcaacgtca ctggtgtcag
     2701 cttcatgtca gtggatcttg cggcgaagca gcttgggctc ttgcgtgagt tgcggccggg
     2761 agccgcgcgc attggcctgc tcgtcgatcc caaaatggcc ccttaccgag ccgtttgtct
     2821 cacaagtccg ggctgcggct tcgaccatgg ggcagcaaat cgacgtcctc tacgtcagca
     2881 gcgaccgcga gatcgagacc gcctttacaa cacttcctgg gtgaggtctg ccccgctgag
     2941 gtccgcagct caacgagcac ctggagcacg aggacggcgc ggtccgcgcg atggccgaac
     3001 aggctgccaa aagtgctgaa aggaatctgc gcaaagttca ccgcgtcgag aaggagtttc
     3061 aagagcttct atcccaactg cgcgctgagt ttgatgcggc gatcgcaaaa gagcgaaagc
     3121 ccgcgccgtg accaaagccc gaactgcacc aagaccatgc ggcggcgagc tggggagggc
     3181 cggatgctga atcgagccgc aatccgccgt cggcggctgg cagagattga acatcagcgc
     3241 ggcgagggga ggtgtgccgg cagatatcga attttgatga atcgaattgg cgctaatccg
     3301 attcatcaat cgacagtcga gcacagggtc gcggctgcct ttgcggatgg gaagtggcgc
     3361 ggcgtgacgg aaatggcgac tgcggcgggg ctgacgacca ggcaaatcgc cagtgcgatc
     3421 cagcagattg aacaaaagcg gctcgcgagt gtcgcgcgcc gtcgcgacgg ttttgactct
     3481 cgcgccaggc aatatcgaat tgtccggctc aagccgggcg acaaaatggt gaccttactg
     3541 taggggcatc cgcgccgccc cccgcgcgcg accaaaaaac ttcatccccc gcggacgagt
     3601 tctgcccctc ccgaagaact tctggcgagg tgatgccgcc tgaaggagca ggggattcag
     3661 cgatggagta ttacagtaac gccgccatcg aaaaaaaaag ctgtggcaaa ccgattcatt
     3721 ggcttgcgac taatcctacg ggtgggatgg tcgatgaggg cgagcgggca tgagtccagg
     3781 tgagggagcc acatggtgaa ctaccgtgga ccgctatgaa cgtgcccgcc gcctaatcga
     3841 agaagaagaa ctaagggtct cgcgcgccgc gcgccggcca gccgacgggt tgctgccccc
     3901 aagccagagg aaagacgctc gcccgcctgc ccctgagccc gacgccgccc catttaccaa
     3961 agaggaactc atcgacggca tcgccgactg cgtcgctgca ttgctggcac agcgcgatga
     4021 gaggatcgcc gagctgggcg ccaagctcga cgcagtgctg caaatcctcg gcactggcaa
     4081 gagtggcgac atcatcgatt tgcccaagaa tttctggcgg cgcgacgcgg cgtgacccca
     4141 agtgagggag cagcacgtag tgggtggcgg caggtggggc gcgcgagatc gttatccagc
     4201 cgcccccgcg ctctgccaaa aaaaattgcc tgcattatcg accgccggcg accggttatt
     4261 gcggttcccg aacagcgcgt gttcgctctg cccccgcccg cgcgcgggag cgcgtaattc
     4321 gatcaaatga aacggcgcga gttcatcacg ctgctcggcg gcgcggcggt cgcgtggccg
     4381 ctcgcgccgc gcgcgcatct atccattcac cgcctttgcc tcgacaggcg gacttctggc
     4441 gtacgacgtc ggggctgccg ctttcgggcc tagcgtgcta tgaatgagcc gagacggtcc
     4501 tgatcgttta ggaccaagcc atggctgaaa agcttgtcgt cgatgttggc ttcgatgcag
     4561 cgcacggcca ttatgtcgcg caccaccccg aattaaagcc gttcaccgcg ctaagccttt
     4621 tcggcctgcg gaagaagatc gaggctgcgt tgttgcccga gaccgtcgac atcaagctcg
     4681 tgctcaaccg cagtgcccga ttggagcgag accgccgccg tcgcagcggt gagcaaggcc
     4741 gcgaccgagc atggccgcgc tgaaaacggt gctcgatgaa gggccaggtg ctcggcttat
     4801 gtccgctttg ggtcataagc agacatcacg ccgtcactta gactatgtcc gctttacccc
     4861 cgaaagcgga catggcgata ggcgcggttg cggttttatt tcagccccgc attctcaagg
     4921 atcgccgtga gcgtcgcacg accgctgtcc gtcagcgcat acaccccggt ctcacgattg
     4981 tgcacgatga atccccggac ggccatggac tgcatcgctt tcgcgacaat tcccaccgca
     5041 gcatggtcga tcccggtcgc ggcgcagaac aggatcactc ggtcctgcgc gctgaggttc
     5101 gtcgtcagct ttgccatgtc tactctcgcc gccactcgta gccgtgcgtc ggggtgcggt
     5161 cgccatgatg cccgaatacg aggccgccaa ctgaggcggc ctttctcatt actttacttt
     5221 cttcctgtcc ggttgcttgc ttcagctacg cggcatatgc gctttcagca acggtccgat
     5281 tttgagattt cgctatattg acatttttgc agattccgca aattaggaca gtcaaatttt
     5341 taaattagga ccaaggcttc ggaattagga cactaccggc cccgatctcg cgattgtgcg
     5401 cgatgaaatt cttggacttg atattcaatg gactgcaacc tgcgccccac ccaccggact
     5461 atgccaatca cgaggcttcc gacaagccac agcaacgcga ccggccccaa gaaaacaccc
     5521 cacaagatag cgctacggta gttttcttcc agcaaagcgt gagctaggcc aaccggcgac
     5581 atgtatccag cgtcgctcaa gcaccaatct ttcgaatggc gttcccaaac gtcggcctcg
     5641 cggcgatttc ctatcgcccg caactgagta ccggttatca aactcgattt catacacgct
     5701 gcataggatt tgcttgcagc tttattctta gtgacgagta aataggttgg tgacgctatt
     5761 agccagagga ccgagatcac cacgccaatc ctctgccatc cgctcattca agttgcccct
     5821 gtcgtgccgt cccccgcacg actccgagcg ggctggtgac ggcgcttagt tccgctcctg
     5881 taccacgagc catcggcgag cacgtcgaaa tcgtcgttca ttccactcgc cggaagagcg
     5941 ggaagccgag gcgcgcttga ggatcgggta gtccttgtcc attgggggcc taattcggcc
     6001 tgcctagata gttcactcct ggcattcggc tagagaaggg ttctcgtcac agagatgttc
     6061 taaccttatt tctcgttcgt cccgcagcaa gtgagtgatg gccaagagcg cgagcaggat
     6121 accgagggca atccaaatat tgatttgtcg catcagaata gctcgctcgc ccgcctcggg
     6181 ggccttcatg cggtatcgat tgtcgtaacg tcgagcttgg catcataaaa gagcgcgagt
     6241 tcgatctgcc gggtgagacc ggcgacatcg gcctccgcta acagcaattc ggcggcgcgt
     6301 tgccattgcg cccttgtctc ccgctgcttt gagagcttga gcatgtaggc gcgcgcgtcc
     6361 tcgatggtgc gcagcgtgcc gccgtccttg gtgggcaatg ccttgcttag cttcgtacgg
     6421 aggtatgcgg gacgcaacat cccgtaccac ttaggattag ggcgcgggcg agtcgagtcg
     6481 cgtcgcacct tgcctgatca gtgccttcgg atagtatcgg ggcgtctaga ggctcaggtc
     6541 tctaggcggt ttctcctttg ctagagacaa ctcgaagccc cggccacgtc cggggctttc
     6601 tttaactctc agcctcgcgg ctttttcggc ggcagcttgt gcgccagctc gggccactcg
     6661 ctttcaacca cgcttcgcat ttcgtccgag atgtgatcga tccactcgat atcgagcatg
     6721 tagtggcgct gccaaaaaag aaccctgctg cgggcggggc ctcaatatgg cggaccatag
     6781 tatcgccgcg gcggaccata gtagggcgat ggaccatagt atcgcgggga cggaccatag
     6841 tatcgcgggg actgagcgta gaaccccggg catggtgcgc cgcgaaggca gtcctctaag
     6901 tagacggcat gtgctggtgt cgccagcgtc gccgccgcag cggtcaagcc taaggcaatc
     6961 agaagcgttt tcatgacagc tctcattttg gcgcgtcaat gggctccgct tgggtaactt
     7021 acagagcgtg ccagaggttc ctcacggaac gtccggcacg aacaaggcgg cattcagtgc
     7081 aagtccttcg gcggcagctt gtacgccaac tcgggccact cgctttcgac cacggctcgc
     7141 atttcatccg agatgtggtc gatgtgttcg acctcccgca tgtacttgtg gaggcgcttt
     7201 aggtctgccg gcatcatgac cttgtcgccg acgatgcggg gcttatagac gcgccgttct
     7261 ttactcattg ccgcctactc tcattgatca cgcggacacc ctgacactgg gtaacgaccg
     7321 cttccggcca tagtttatgg ccgcccgatg ccaccatggc tgcaacgaac aagtgtttgg
     7381 cgcagagtaa caagtcccgc acggggggcg aagcgactaa gaagcagaaa ccgccaacga
     7441 acgggtagca acagggctgc gcccgtgcgg tcggctgcgc gaggtttgcc ccaactcgat
     7501 gggggtcgac cactgcttcg cctgcaggac agcgacaaca agacctgcgc gctgtgcggg
     7561 cactcctgga agtcaaacag aagaacggcg cttatgcgcg tgcgtgcact gcggctgcgg
     7621 atgcaaggat gtgatgcgat gaaactgact atccatgacg gcgaacttgc gcgttatctc
     7681 gtacattgca aacacgcggg caaaccgaag cgtgttttcg tgccctacga atccgacgtg
     7741 cttgtatttg cgacaagccc ggaagacgcc aagatcgctg cggccgagaa attgcaggac
     7801 aagtggcttc gacatcgcgg cgttcatacc gaatggcgcg caaccgacgt tcgattggat
     7861 tggtctacac gctagcgatg attgaggaga cgccgccatc gcagccgcgc actcttacgc
     7921 ttaatcgttt tcgtcagcgc ggtttctcag tgccgtgcgg catcgtttag ttccgcactg
     7981 aaaataaagg gggcgccgga ccctctagac aaagaaagcc ccgggcaaag ggaccgaggc
     8041 ttcaggtagt ctctaaccta ggagaatggc ctagagggaa tgagcctcta gacgctccga
     8101 tactatccca aaacacacaa ggcgcggatc acgtaagcgc ccgacccgcc ggcggggcca
     8161 cccatcgacg gctcaggcct ggccacgtcg gcccaggaag aggaaagggg cgcgtcatgg
     8221 ccaactggcc aacgccacac ttgtcgcctg gttcggtacg gagaattgct a
//
LOCUS       DATLJT010001102         1784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4443177,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001102 DATLJT010000000
VERSION     DATLJT010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4443177"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VM910_25485"
     CDS             <1..557
                     /locus_tag="VM910_25485"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVI65910.1"
                     /translation="NGIHPARPHNVVATVEEYDPAANSWKTVRPLLLARNHHVAVGVG
                     DKIYVIGGRVGAAFISSASNNVDLVEMYDPATDLWTPRTRMPTARSAIGAGVYNNQIL
                     VAGGEGQDQRFLAAFKAVEAYDPVLNRWQVLPSMPHPRHGLAVGAVGNRLYTVSGDGQ
                     SAGNGIEHSAVAFNEILQLDLVLK"
     gene            complement(683..1120)
                     /locus_tag="VM910_25490"
     CDS             complement(683..1120)
                     /locus_tag="VM910_25490"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HVI65911.1"
                     /translation="MTLRARSFAAAVALLAFTLARSSALAQTRTLYDRLGGYPAISAV
                     VDDFVKNVAADKRINRFFANANIDRLKARLVEQICQGTGGPCVYSGRDMRSVHAGMGI
                     RGKDFDALVQDLAKSLNKFKVPAREQKQLVAVLAPMKKDIVTR"
BASE COUNT          320 a          577 c          575 g          312 t
ORIGIN      
        1 agaacggcat ccacccggcg cgcccgcaca acgtggtggc gacggtcgag gaatacgacc
       61 ccgcggcgaa cagctggaaa accgtgcgcc cgttgctgct cgcccgcaat caccatgtgg
      121 cggtcggggt cggcgacaag atctatgtga tcggcggccg cgtcggcgca gccttcatct
      181 ccagtgccag caacaacgtc gatctggtgg agatgtacga ccctgcaacc gatctgtgga
      241 cgccgcgcac gcgcatgccc acggcgcgca gcgccatcgg cgccggggtc tacaacaacc
      301 agatcctggt cgccggcggc gaaggccagg accagcgctt tcttgccgcc ttcaaggcgg
      361 tggaagccta tgaccctgtg ctcaaccgct ggcaggtgct gccgtccatg ccgcatccgc
      421 gccacgggct tgcggtcggc gcggtcggca atcgcctcta tacggtgagc ggcgacggcc
      481 agtcggccgg caacggcatc gagcactccg ccgtcgcctt caacgagatc ctgcagctcg
      541 acctggtctt gaagtgacgc gctgtgggtc tcgcaacagc catcgtgcaa gaaggctgct
      601 gacagggccg cgaactcggt gaactccctc ccccgcttgc gggggagggg acgctggagc
      661 cgatggtcct ctgcctgagg tgtcagcgcg tcacgatgtc cttcttcatg ggcgcgagaa
      721 ccgcgaccaa ttgtttctgt tccctcgccg gcactttgaa cttgttgagg ctctttgcga
      781 ggtcctgcac cagcgcgtcg aagtccttgc ccctgatgcc catgccggca tgcaccgacc
      841 tcatgtcgcg gccgctatag acgcaggggc cgccggttcc ctggcagatt tgctcgacca
      901 ggcgcgcctt gagccgatcg atattggcgt tggcgaaaaa cctgttgatg cgcttgtcgg
      961 ccgcgacgtt cttcacgaag tcgtcgacca cagccgaaat ggcgggatag ccgccaagcc
     1021 gatcatagag cgttcgcgtc tgggcaagcg cgctgcttcg agcaagcgta aatgcgagca
     1081 gcgcgacggc ggcggcgaac gagcgagctc gaagcgtcat ggtggtcctc caggggtgtg
     1141 atggcgtttt cagcggttgc ggaggcgcaa ggctcctccc gagcgcagat acggagcgac
     1201 cggcaaaacg gtttcaccgc cggctcgaaa tcccgcgcga gccgcgttga gggtcgaggc
     1261 acgccctgtc tcaatatgcg agcaggcgat gaaggcggct tccccgcgcc gccaagcgcg
     1321 gcgaatcaaa gcggccggcg tcgaagcgcg ctcgccgctc gatcaaggct caagagaatc
     1381 gccgtcgctg atgaccgaaa atccgcgcga agacgcggtg agccggcgca ttcggcgcaa
     1441 gcccgcgtcg cccgcccgcg tcgcgcgcga gacggcttgc gcaggcccgc cctcacttgg
     1501 acggagggac gtgctgataa tttctcgctt gacatcaatt tcgtgacagg cgcgtgaccg
     1561 ggctgtgaat ggaacccttc cgaatccgcc gccgatatcc gatacttgct gcaccgcacc
     1621 tgagtcgccc ccgaatttca caagcttggc accacattta gcagtttgtt tacggggggc
     1681 tactaagcct tgacgaaccg aaggtgctgt cctagcttga aaaggttcgg cgcgagtggt
     1741 tcgaagtccc gccgggcgct ctcctgaaag ggtctgcgta accg
//
LOCUS       DATLJT010001103         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1686232,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001103 DATLJT010000000
VERSION     DATLJT010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686232"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /gene="serA"
                     /locus_tag="VM910_25495"
     CDS             <1..1468
                     /gene="serA"
                     /locus_tag="VM910_25495"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HVI65912.1"
                     /translation="EIIGNYDGLAIRSATKATAKIIEKAAKLKVIGRAGIGVDNVEIP
                     AATAKGIIVMNTPFGNSITTAEHAITLMLALAREIPQADASTQAGKWEKNRFMGVEIT
                     GKVLGVIGCGNIGSIVADRALGLRMRVIAYDPFLSPERAKDIGVEKVDLDELLRRADF
                     ITLHTPLTEKTRNILDAAALAKTKKGVRIINCARGGLVDEKALVAALDAKHIAGAAFD
                     VFSEEPATSNVLFGHPHVICTPHLGAATTEAQENVALQVAEQMSDYLLQGAISNAVNF
                     PSITAEEAPKLKPFIALAEKLGSFAGQLTETGILKVQITYEGHVAEMKIKALTSAVLS
                     GLLRPMLGDVNVVSAPVVAKERGMVVDEVVRAAHSDYESLITVTVTTERQERSVSGTV
                     YADGKPRLVDIKGIRVDAEFGRSMVYVTNEDKPGFIGHFASLLGDAKINIATFHLGRS
                     APGGDAIALVEVDGAVPPDVLAKVQALPQVKQAKVLLF"
     gene            1732..2403
                     /locus_tag="VM910_25500"
     CDS             1732..2403
                     /locus_tag="VM910_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65913.1"
                     /translation="MKRVVLAAMIGLIALAGAARAETPVAVVEDVYGKVTGVEFMDYV
                     VPGQVIKLGPGGMIVLGYMKSCWRETISGVGTVIVGTEQSAVHLADFKAGKVPCDTAQ
                     MEKLPKEVGESAATVVRSLKENPPAGPPPLLLHGTSPIVATSDRGKLVVERIDVKGER
                     YEVDLAHAALAHGKFYDFAKANTALTPGGVYAATLKSKRQVFQIDPEATAGTGPVIGR
                     LVSLQ"
     gene            2404..>3510
                     /locus_tag="VM910_25505"
     CDS             2404..>3510
                     /locus_tag="VM910_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HVI65914.1"
                     /translation="MRRFSQNNAMGRFIRRDVMAIALVALLSGIAFTLPPLDLVHGWS
                     IDVLTALRYKLFGTHRDPAAAPVAVIAIDEETYETPPFKGSPTPVWTTEIGRVLSAVL
                     DGGAKVAGFDIVFSTSIEQSEIPFGDDVVGTKLRGFDRPFLRALAGGAAAGKVVLGEV
                     WRTEGAIAPSPGQRIAVRQQQNIRPLNVYSDPDDVVRRVPLTFPGNGKPVPSMALELA
                     ARAQGAEPVFAADGSVTLAGYRIPSAVKNTLTLNFDGGGNDVLTYSLADLHACVEGND
                     KDFFHRQFDGKVVIFGTLLDSLDRKLTTKRFVTGIDRSGAPRCMPAAAPAPAAAFSRA
                     SIAGVYIHATAVNNLIARDAFVELGRWQTAMIAIG"
BASE COUNT          611 a         1133 c         1152 g          614 t
ORIGIN      
        1 cgagatcatc ggcaattacg atgggctcgc gatccgctcc gccaccaagg cgaccgcgaa
       61 gatcatcgag aaggcggcga agctgaaggt gatcggccgc gccggcatcg gcgtcgacaa
      121 tgtcgagatt ccggccgcca cggcaaaagg catcatcgtg atgaacacgc cgttcggcaa
      181 ttcgatcacg accgccgaac acgcgatcac cctgatgctg gcgctggcgc gcgagattcc
      241 gcaagccgac gcctccacgc aggccggtaa atgggagaag aaccgcttca tgggcgtcga
      301 gattactggc aaggtgctcg gcgtcatcgg ctgcggcaat atcggctcga tcgtcgccga
      361 ccgcgcgctg ggcttgcgca tgcgtgtgat cgcctacgat ccgttcctgt cgcccgagcg
      421 cgccaaggac atcggcgtcg agaaggtcga tctcgacgaa ttgttgaggc gtgccgattt
      481 catcacgctg cacacgccgt tgaccgagaa gacgcgcaac atcctcgacg ctgcggcgct
      541 cgccaagacc aagaaaggcg tgcgcatcat caattgcgcg cgtggcggcc tggtcgacga
      601 gaaggcgctg gtcgccgcgc tcgatgcaaa acacatcgca ggtgcggcct tcgacgtgtt
      661 ctcggaggag ccggcaacga gcaacgtgct gttcggccat ccccacgtca tctgcacgcc
      721 gcatctgggc gctgccacca ccgaagcgca ggagaacgtc gcgctgcagg ttgccgaaca
      781 gatgtccgac tatctcctgc agggggcgat ctccaatgcg gtcaacttcc cctcgatcac
      841 ggcggaagag gcgccgaagc tgaagccctt cattgcgctg gccgaaaagc tcggctcctt
      901 cgcgggacag ctcaccgaga ccggtatcct caaggtgcag atcacctatg agggccatgt
      961 cgcggagatg aagatcaagg cgctgacgtc tgcggtgctg tccggcttgc tgcgccccat
     1021 gctcggcgac gtcaacgtgg tctccgcgcc ggtcgtggcc aaggagcgcg gaatggtggt
     1081 ggacgaggtg gtgcgcgcag cccacagcga ctacgaaagc ctgatcacgg tcaccgtcac
     1141 caccgagcgg caggagcgct cggtgtcggg tacggtctac gccgacggca agccgcgact
     1201 cgtcgacatc aagggcatcc gcgtcgatgc cgaattcggc aggtcgatgg tctatgtgac
     1261 gaacgaggac aagccaggct tcatcggcca tttcgcgagc ctgctcggcg atgccaaaat
     1321 caacattgcg acctttcatc tcggtcgcag cgccccgggc ggtgacgcga tcgctctggt
     1381 cgaggtcgac ggcgcggtgc cgcccgatgt gctcgccaag gtgcaggcct tgccacaggt
     1441 caagcaggcc aaagtgctgt tgttctgagg ttggcgcaat cggagccggg gggcggggga
     1501 ttgcgtgcgg cgcggtgatg gcgccgccgt tcgatcaaat tcgtggggcc ttgatacgac
     1561 agagcagggg ccgttttgtc gcggtctccc gcaggaaaag atttcgacct tgtgtgatat
     1621 tgctcacgat gcttgcgcgg gcttgaggct aggtgttcgg gcaaagaggt cttgggccgc
     1681 cgattcctga tctgatagaa gcgggggccc tgtttcatcg aggttcgaaa catgaaacgc
     1741 gtcgtgcttg ccgccatgat tggcttgatc gccctcgctg gcgccgcgcg ggccgaaacg
     1801 ccggtcgccg tggtcgagga cgtctatggc aaggtcacgg gcgtcgaatt catggactat
     1861 gtcgttccgg gccaggtgat caagctcggg cccggcggca tgatcgtgct cggctacatg
     1921 aaatcctgct ggcgcgagac gatctccggc gtcggcacgg tgatcgtggg caccgagcag
     1981 agcgccgtgc atctcgccga tttcaaggcc ggcaaggtgc cctgcgatac cgcccagatg
     2041 gaaaagctcc ccaaggaagt cggcgagagc gcggcgaccg tggttcgcag cctcaaggag
     2101 aacccgccgg ctggtccgcc gccgctcctg ctgcacggca cctcgccgat cgtcgcgacg
     2161 tcggatcgcg gcaagctcgt cgtcgaacgg atcgacgtga agggcgagcg ctatgaggtg
     2221 gaccttgccc atgccgcgct ggcccacggc aagttctacg attttgccaa ggccaacacc
     2281 gcgctgacgc cgggcggcgt ctatgccgcg acgctgaagt cgaagcggca ggtgtttcag
     2341 atcgaccccg aggccaccgc cggcacaggc ccggtgatcg gccgcctggt gtcgctgcaa
     2401 taggtgcgcc gcttcagcca gaacaacgcg atgggccgtt tcatcaggcg ggatgtgatg
     2461 gcgatcgcgc tggtcgcgct cctgagtgga atcgccttca ccttgccccc gctcgatctc
     2521 gtccacggct ggtcgatcga cgtgctcacg gcgctgcgtt acaagttgtt cgggacgcat
     2581 cgcgatcctg cggcagctcc cgtcgcggtg atcgcgatcg acgaggagac ttacgagacg
     2641 ccgccgttca agggctcacc gaccccggtc tggaccaccg agatcggccg cgttctctcg
     2701 gcggtgctcg acggcggcgc caaagtcgcc ggcttcgaca tcgtcttctc gacctcgatc
     2761 gagcagtccg agattccctt cggcgacgat gtggttggga cgaagctgcg cggcttcgat
     2821 cggccgttct tgcgcgcgct ggcagggggc gctgccgccg gcaaggtcgt gctcggcgag
     2881 gtctggcgca ccgagggcgc gattgcgcct tcgcccgggc agcgcatcgc cgtgcgacag
     2941 cagcagaaca tccgcccgct caacgtctat tccgatcccg acgacgtcgt ccggcgcgtg
     3001 ccactgacct tccccggcaa cggcaagcct gtcccctcga tggcgctgga gctcgctgcg
     3061 cgcgcgcagg gcgccgagcc tgtcttcgcc gccgacggca gcgtcacact cgcagggtat
     3121 cgcattccga gcgccgtgaa gaacacgctg acgctcaact tcgacggcgg cggcaacgac
     3181 gtcctgacct attcgcttgc cgatctgcac gcctgcgtcg aaggcaacga caaagatttc
     3241 ttccaccgtc agttcgacgg caaggtcgtc atcttcggca cgcttctgga ttcgctcgac
     3301 cgcaagctca ccacgaagcg cttcgtgacg gggatcgacc gctccggcgc tccgcgctgc
     3361 atgccggctg ccgcgcccgc gccggctgcg gcgttcagcc gcgcgtcgat tgccggcgtc
     3421 tatatccacg caaccgccgt caacaatctg atcgcgcgcg acgcgttcgt cgagcttggc
     3481 cgctggcaga ccgcgatgat cgcgatcggc
//
LOCUS       DATLJT010001104         6903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2835423,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001104 DATLJT010000000
VERSION     DATLJT010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835423"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VM910_25510"
     CDS             <1..128
                     /locus_tag="VM910_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HVI65915.1"
                     /translation="PVEGSATLTDAQAADLQAGKLYINVHTAANPGGEIRGQVTK"
     gene            397..2205
                     /locus_tag="VM910_25515"
     CDS             397..2205
                     /locus_tag="VM910_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65916.1"
                     /translation="MAVPAPAILDVRTDKPDAADAARWWRGIGVRQVRLVCGLIMFSY
                     IFSHFFNHALGNLSYDAMETWLRFHVWWWRIPVVNITLYAAAATHLSLGLWAHYQRRN
                     FRYTAIEVTQLVFGLSIPLLIASHFGIMRLGGLLFGRPVPPYSGVLLAYWVNRPYMVA
                     VQFFLLTVAWIHACIGLYFWLRLKPFFKWAAPFLLAIAVLLPPLAMTGTHRGAHETVE
                     RAAQPEWRAEHIKLTPPAQRSVIEDITLYYFPILYGTAILLVFVARGVRALIENRRGS
                     ITVSYPGRKVRSPRGLSILETSLRFKVPHASVCGGKARCSTCRVRVVSDRFALPPPSS
                     RESHVLARVGASSNPSIRLACQLRPKADIAVIPVLPANIGADFVRNRRRINVGEEHYV
                     VSMFVDMRGSTKLAEARLPYDTVFLVNRFLEAASQAVIDAGGQPNQFVGDGLLALFGL
                     RTDPTTASRQAMRAAAIVAANVAYMNHEFSTELPQPIEFGIGIHGGEVIIGDIGFRDH
                     TVFTALGDAVNVAARLQDLTKTLGCSVMVSEEVYRQAGIAPGRIAPAEASIRGRDRPV
                     QVYAFTDPSVLASLLDEQDAEHEPSNHATAKAAISA"
     gene            complement(2192..2641)
                     /locus_tag="VM910_25520"
     CDS             complement(2192..2641)
                     /locus_tag="VM910_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI65917.1"
                     /translation="MKRTIVAMGVLLVGAGAVMADQKLAVEQDNLMRSQGKAMYVVIG
                     KMIKGEIPYDQKTVDNAIADLEASVPKIPKMFETNPKEDVVNASYGSSPKVWQNKADF
                     DSKVPPVQKAIADVKGKIKNLDQLKTAYTSINDRCTDCHETYRLKLK"
     gene            complement(2788..5094)
                     /locus_tag="VM910_25525"
     CDS             complement(2788..5094)
                     /locus_tag="VM910_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI65918.1"
                     /translation="MATPIKFGVGQSVLRKEDDALIRGKGRYTDDVAAQSSLHALVLR
                     SPHAHATYTINAANARALPGVALILTAEDVKDLGDLPCLFQLETNPFTGPPYPILANE
                     EVRHVGDVVAFVVADTVDHARDAIEAIEVNWTPLPAVAGVANAVKPGSPQVWSSHAGN
                     ILFDVPIGDKTATDAAFAKAHAVAEIRIVNPRVVASFMETRAAVCEYDAKRDYLTLTV
                     GSQGSHRLRDILCQNVLKIPIEKMRVICPDVGGGFGTKLFPYREYALLAVASRKLKKT
                     VRWAADRSEHFMGDAQGRDNVTTARMALAEDGKFLAMDVDLMGDMGAYLSTFGPYIPH
                     GGAGMLPGLYDIQTFHCRVRTVFTHTVPVDAYRGAGRPEAAYVIERLVDASARKLGMT
                     PEAIRRKNFISPRALPYTTATGKIYDSGDFTAHMKRAMEVANWKDFSKRAKQARKQGL
                     VRGIGMASYVEICGTMGGDETAHVRLDPNGDVTILIGTQSSGQGHQTAYAQIVAQQFG
                     LAPERVHIHQGDTAEIATGLGTGGSSSIPTGGVCVERATRELGKQLKNLAAEALETSA
                     GDLEIDNGVVRIAGTDRSVSFADLAKRGDSSKLNGSATFTSADGTYPNGTHIAEVEID
                     PATGIIKIVNYVIVDDFGVTLNPLLLAGQVHGGVMQGIGQALMEQVVYSPTDGQLVTG
                     TYMDYALPRASDGPSFHFETHNIPCKTNPMGVKGAGEAGAIGSCPAVVNAIVEGLWRE
                     YKIDHIDMPATAERVWIAIRERQRQHSL"
     gene            complement(5159..6688)
                     /gene="murJ"
                     /locus_tag="VM910_25530"
     CDS             complement(5159..6688)
                     /gene="murJ"
                     /locus_tag="VM910_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HVI65919.1"
                     /translation="MLGRIFTVGFYTLLSRLTGFARDIMLAAILGAGPVADAFFVAFR
                     LPNHFRAIFAEGAFNAAFVPAYAHVHGERGDTSARLFADRIFTLLFLSQVILLVVAWL
                     FMPQAMSVLAPGFTEDAEQRRLAIDLTRITFPYLLLITLVTLYGGMLNVMQRFASAAA
                     ASIFLNIAMMATLALAAFFPTAGHAAAWGVLISGFLQYFLLAGDLATHGGLPRFAPLK
                     LDEDIRAFFRALGPATLGSMGTQVAMFADTIIATFLPAGALSALYYADRLNQLPIGVI
                     GIAIGTVLLPEMSRRLTADDHAGATASQRRAFDFTLLFSVPFVAAFLAVPDAITRAMF
                     ARGAFTKGDAAAAGATLAAYAVGLIPFVLIRSAVATFYARKDTATPVKASLTGLAANV
                     ALKLLLMGPLAQVGLAFATAVGAWINLLLVLWFAVRANHLAFDRDLVQSLAKFFGSGV
                     VLALAFWLTRKFAAAHLAATSAFRDETILLLLIAVGTVIYAASILLLFGPRWLRSLMR
                     G"
BASE COUNT         1240 a         2187 c         2208 g         1268 t
ORIGIN      
        1 gcccggtgga gggcagcgcg acgctgaccg atgcccaggc ggccgaccta caggccggca
       61 agctctatat caacgtccac accgcggcca atccgggcgg cgaaatccgc ggccaggtga
      121 ccaaatagcg ggaaggtccg aataagccag gtacccgcaa ggcgtttcat ttgatcagag
      181 ggggacggac gcatcgcgcg tccgccccct ttttcgtgcg gtgccggatg tcggcggcgg
      241 gtcgcagcat gcgctctgac cttaccttaa cgatcgcagc gcacgccctc tcgccaatgg
      301 ggccggcttg cgttagcctg cctccggctt gcggcgccgc aaaaagtgcc gggagccggt
      361 tttttctaga ggttttgctc acaaatcgat ttccgcatgg ccgttccggc acccgccatt
      421 ctcgatgtcc gcaccgacaa gccagacgca gccgacgccg ctcgctggtg gcgcggcatc
      481 ggtgtgcgcc aggtgaggct ggtctgcggc ctcatcatgt tttcctacat cttctcgcat
      541 ttcttcaatc acgcactggg caacctctct tatgacgcca tggagacgtg gctgcgcttc
      601 catgtctggt ggtggcggat ccctgtcgtc aacatcacgc tctacgccgc cgctgcgacg
      661 catctttcgc tggggctgtg ggcccactat cagcgacgaa acttccgcta cacggccatc
      721 gaggtgaccc agctcgtgtt cggattgagc attccgcttc tgatcgccag ccatttcgga
      781 atcatgcggt tgggagggct gttgttcgga cgcccggtgc cgccgtattc gggggtgcta
      841 ctggcctact gggtcaaccg cccgtacatg gtggccgtgc aattcttcct gttgacggtt
      901 gcctggatcc acgcctgcat cggactttac ttctggctcc gcttaaagcc gttcttcaaa
      961 tgggctgccc cgttcctgct cgcgattgcg gtcttgctgc cgccgctggc aatgaccggc
     1021 acccatcgcg gcgcccacga gacggtcgag cgcgcagcgc agccggaatg gcgagccgag
     1081 catatcaaat taaccccgcc ggcgcagcgc tcggtgatcg aggacatcac gctctactac
     1141 ttcccgatcc tgtatgggac ggcgatcctg ctcgtcttcg tcgcgcgcgg cgtgcgggcg
     1201 ctgatcgaaa accggcgcgg ctcgatcacc gtttcctatc ccggtcgcaa ggtccgtagt
     1261 ccacgcggcc tctccatcct ggagacgagc ctgcgtttca aggtcccgca tgcaagcgtc
     1321 tgcggcggca aggcccggtg ctcgacctgc cgcgtgcgag tggtaagcga tcgctttgcg
     1381 ctgccgccgc cctccagccg cgaatcgcat gtgctggcgc gcgtaggagc gagctccaat
     1441 ccgtcgatca ggctcgcctg tcagcttcgg ccgaaggcgg atatcgccgt catccccgtg
     1501 ctgccggcca atatcggtgc cgacttcgtt cgcaatcgcc ggcgcatcaa cgttggcgaa
     1561 gaacactatg tggtcagcat gttcgtcgac atgcgcggat cgaccaagct tgcggaagcg
     1621 cgtctacctt acgacaccgt gttcctggtc aaccgctttc tggaagccgc ctcgcaggct
     1681 gtgatcgatg ccggcgggca gcccaaccag ttcgtcggcg acgggctgct cgcgctgttc
     1741 gggctgcgca ccgatccgac caccgcctcg cggcaggcga tgcgggcggc cgcgattgtc
     1801 gccgccaatg ttgcctacat gaatcatgaa ttttcaaccg aactgccgca gccgatcgag
     1861 ttcggcatcg gcattcacgg cggcgaggtc atcatcggcg atatcggttt ccgcgatcac
     1921 accgtcttca ctgcgctggg cgatgccgtg aatgtcgcgg cgcgcctgca ggacctcacc
     1981 aagacgctgg gctgcagcgt gatggtgtcc gaggaggtgt accggcaagc tggcatcgcg
     2041 ccgggccgca ttgcgccggc ggaagcgtcg atccgcgggc gcgatcggcc ggtccaggtc
     2101 tatgcgttca ccgacccgag cgtgctggcg agcctgctcg acgagcagga cgccgagcat
     2161 gagccctcca atcacgcgac ggcgaaggcc gctatttcag cttgagacga taagtctcgt
     2221 gacagtcggt gcagcggtcg ttgatcgacg tgtaggcggt cttgagttga tcgaggttct
     2281 tgatcttgcc cttgacgtcg gcgatcgcct tctgcacggg agggaccttg gagtcgaagt
     2341 cggccttgtt ctgccagacc ttgggcgatg agccatagct ggcgttgacg acgtcctcct
     2401 tcggattggt ctcaaacatt ttcggaatct tgggaacgct ggcttcgagg tcggcgatgg
     2461 cattatcgac ggtcttctgg tcgtagggaa tctcaccctt gatcatcttg ccgatgacca
     2521 cgtacatggc tttgccttgg gaccgcatga ggttgtcctg ctcgacggcg agcttctggt
     2581 ccgccatgac ggcgccggcg cccacgagca acacacccat cgcaacgatt gtccgtttca
     2641 tcgacgactt ctccttcgaa aacccgattg caaacgatgc cgcgatcccg ctcccgcgga
     2701 atcacggctg cctctcttct gatgccccgc ccctcatccg tacgaacccc gccgcggcgt
     2761 tttattccct gcggcagggc tgtgatgtca aaggctgtgc tgccgctggc gctcgcggat
     2821 cgcgatccag acacgctcgg cggtcgccgg catgtcgatg tggtcgatct tgtactcgcg
     2881 ccacagccct tcgacgatgg cgttgaccac ggccggacag gagccgatcg cgccggcctc
     2941 tcccgcgccc ttgacgccca tcgggttggt cttgcaggga atgttgtggg tctcgaagtg
     3001 gaatgacggc ccatccgatg cgcgcggcag cgcatagtcc atgtacgtgc cggtgacgag
     3061 ttggccgtcg gtcgggctat agaccacctg ctccatcagc gcctgcccga tcccctgcat
     3121 cacgccgcca tgcacttgcc ctgcaagcag cagcggattg agggtcacgc caaaatcgtc
     3181 gacgatgacg taattgacga tcttgatgat gccggtcgcg gggtcgatct cgacctcggc
     3241 gatgtgggtg ccgttcggat aggtgccgtc ggcgctggtg aaggtggcgc tgccgttcag
     3301 cttcgaagaa tcgccgcgct tggccaaatc cgcaaagctg acggagcgat cggtgccggc
     3361 tatccgcacg acgccattat cgatctcgag gtcgccggcg ctggtttcca gcgcctcggc
     3421 cgcaagattc ttgagctgct tgccgagctc gcgcgtcgcg cgttcgacgc agacgccgcc
     3481 ggtcgggatc gaggacgatc cgccggtgcc gagcccggtc gcaatctcgg cggtgtcgcc
     3541 ctgatgaata tggacgcgct ccggcgccag tccgaactgc tgggcgacga tctgcgcgta
     3601 ggccgtctga tggccctgcc cgctcgattg cgtgccgatc aggatagtga catcgccgtt
     3661 cggatcgagc cgcacatggg cggtttcgtc cccccccatg gtgccgcaga tctcgacata
     3721 gctcgccatg ccaatgccgc gtacgagccc ctgcttcctt gcctgcttgg cgcgcttcga
     3781 aaagtctttc cagttcgcga cttccatcgc ccgcttcatg tgggcagtga agtcgccgga
     3841 atcgtagatc ttgcccgtcg cggtcgtgta gggcagcgcg cgcggcgaga tgaagttctt
     3901 gcgccggatg gcctccggcg tcatgccgag ttttcgtgcc gacgcatcga ccagacgttc
     3961 gatcacatag gcagcttccg ggcggcccgc gccgcgatag gcgtcgaccg gcacggtgtg
     4021 ggtgaacacc gtgcggacgc gacagtggaa ggtctggatg tcatagaggc ccggcagcat
     4081 gccggcgccg ccgtgcggaa tatagggtcc gaacgtcgac agatacgcgc ccatgtcgcc
     4141 catcagatcg acgtccatgg cgaggaactt gccgtcctcg gccagtgcca tccgcgcagt
     4201 ggtgacattg tcgcgcccct gcgcatcgcc catgaagtgc tcgctgcgat cggcggccca
     4261 gcgcacggtt ttcttgagct tgcgcgaagc gaccgcgagc agcgcatatt cgcgataggg
     4321 aaacagcttg gtgccaaagc cgccgccgac atctgggcag atcacccgca ttttctcgat
     4381 cgggatcttc agcacgttct ggcacaggat gtcgcgcagc cgatgactgc cctggctgcc
     4441 cacggtgagc gtcaggtaat cgcgctttgc atcatattcg cagacggccg cgcgcgtttc
     4501 catgaagctt gcaaccacgc gcggattgac gatcctgatt tcggcaacgg catgcgcctt
     4561 ggcaaaggcg gcatccgtcg ccgtcttgtc gccgatcggc acgtcgaaca ggatgttgcc
     4621 ggcatggctc gaccagacct gcggagagcc aggcttgacc gcgttggcga caccggcgac
     4681 ggcaggcaac ggcgtccagt tgacctcgat cgcttcgatc gcgtcgcgcg cgtgatcgac
     4741 ggtatcagct accacgaagg cgacgacgtc gccgacatgg cgaacttcct cgttcgccag
     4801 gatcggatag ggcgggcctg tgaagggatt ggtctcgagc tggaacaggc acggcaggtc
     4861 accgaggtcc ttgacgtcct cggccgtcag gatcagtgca acgccgggaa gcgctcgcgc
     4921 gtttgccgca ttgatcgtgt aggtcgcatg ggcatgcggc gagcgcaaaa ccagcgcatg
     4981 cagcgacgac tgtgccgcca cgtcgtcggt gtagcggccc ttgccgcgga tgagcgcatc
     5041 gtcctccttg cgaaggacgc tctggccgac gccgaatttg atgggagttg ccatttatct
     5101 ccaccccgtt tgaactttcc ggcatattgc agtggtttca ggggaagcgc aaacggcttt
     5161 agccgcgcat caggcttctc agccacctcg ggccgaatag cagcaaaatc gaggcagcat
     5221 agatcaccgt gccgacggcg atcagcaaca gcagtatggt ctcgtcacga aacgcgctgg
     5281 tcgccgccag atgcgccgca gcaaacttcc tggtgagcca gaacgccagg gccagcacca
     5341 cgccgcttcc gaagaatttc gcgagcgatt gcacgaggtc gcggtcgaac gccagatggt
     5401 tcgcccgcac cgcaaaccac aagaccagca ggagattgat ccaggcaccg acagcggtcg
     5461 caaatgccag ccccacctgg gcaagcggtc ccatcaacaa cagcttcagc gccacgttcg
     5521 cggcgagccc cgtgagcgag gccttgaccg gggtcgcggt gtccttgcgg gcgtagaagg
     5581 tcgcaaccgc gctgcggatc agaacgaagg gaatgaggcc gaccgcatac gcggcaagcg
     5641 tagctcccgc ggcagcggcg tcgcccttgg tgaaggcgcc gcgcgcgaac atggcgcgcg
     5701 tgatcgcatc gggcacggcg aggaaggccg cgacaaaggg caccgagaac agcagcgtga
     5761 aatcgaaggc gcggcgctgc gaggccgtgg cgccggcatg atcgtcggcc gtgagccgcc
     5821 gcgacatttc cggcaacagc accgtgccga tcgcgatgcc gatcacgccg atcggaagct
     5881 ggttcagccg gtcggcatag tacagcgccg acagcgcgcc ggcgggcaga aaggttgcga
     5941 tgatggtgtc ggcaaacatc gcaacttgcg tgcccatcga gccgagcgtc gcgggcccaa
     6001 gcgcccggaa gaaagcgcgg atgtcttcat cgagcttcag cggggcaaag cgcggcagcc
     6061 cgccatgggt tgcgagatcg ccggccaaaa gaaaatattg caggaagccc gagatcaaga
     6121 cgccccaggc ggcggcatgg cctgcggtcg gaaagaaagc tgcgagcgcg agcgttgcca
     6181 tcatcgcgat gttgagaaaa atcgacgctg cggcagcact ggcaaaacgc tgcatcacgt
     6241 tgagcatgcc gccgtagagc gtgaccagcg tgatcaggag cagataggga aaggtgatgc
     6301 gcgtcagatc gatggcaagc cggcgctgct cggcatcctc ggtgaagccg ggggccagca
     6361 cgctcatggc ctgcggcatg aacaaccagg cgacgacgag caggatcacc tgcgacagga
     6421 acaagagcgt gaagatacgg tcggcgaaaa gccgcgccga tgtatcgccg cgctcgccgt
     6481 ggacatgagc gtaggccggc acgaaggccg cgttgaatgc gccctcggcg aatatcgcac
     6541 ggaaatgatt gggcaggcgg aacgccacga agaacgcgtc cgccacgggg ccggcgccca
     6601 ggatggccgc aagcatgatg tcgcgggcaa agcctgtcag ccgcgacagc agcgtataga
     6661 acccgacggt aaagatgcgc ccgagcatgc gaggcttcta aagcatgaaa gccttggttt
     6721 gaagcgaaga acgaggcagt tccctctccc cgcttgcggg gcgaggcgaa tgtgagttcg
     6781 cggacaaacc ggctaggaaa ctgcgccgcg cacagccgca atgattcggt cctgggtcgc
     6841 ttcgtcgaga taggcgtgca tcggcaggct gatcacgtcg ccggacagtt cttccgagac
     6901 cgc
//
LOCUS       DATLJT010001105         4396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3983699,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001105 DATLJT010000000
VERSION     DATLJT010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983699"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VM910_25535"
     CDS             <1..886
                     /locus_tag="VM910_25535"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HVI65920.1"
                     /translation="ENWAKLPEGRAWGQAIGVDIDRDGTSVWVYDRCGAKTCEGSNIP
                     PIQKFDASGKLVTRFGGGMINWPHGLFADRDGNVWVTDGRGGNGKGHTVVKFSSDGKV
                     LMTLGKPGVAGDGPDTFNGPSDVLVAPNGDIFVADGHGEKTNARIVKFSKDGKFINAW
                     GKAGKGQGEFDVPHALAMDSSGRLFVADRSNDRIQIFDQDGKFLAEWRQFGRPSGLYI
                     DRNDILYAADSQSGEKFNAPFRQGIRIGSAKDGKVVAFITEEGPTPNMPEGVAADKDG
                     NVFGGFTGKQTLKKFVKN"
     gene            complement(1120..1791)
                     /locus_tag="VM910_25540"
     CDS             complement(1120..1791)
                     /locus_tag="VM910_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVI65921.1"
                     /translation="MPATTTFLGELQQLVSLHGYWVVGLMVGLESLGLPLPGETILVL
                     AAIYAATEPSFNIWVVIAVAAFGAIIGDNIGYWLGSRYGYALLLRYGGRIGMFEPRIK
                     LGQYLFLRHGAKVVFLGRFVALLRILAAFLAGLNRMPWRVFLMANATGGVIWAVVFGL
                     GGFMFGKALLQLHHALAPIVFVLALAGFFGCGYLIRSHEDRLTALAERALPGPLVAAL
                     HAKRP"
     gene            complement(3512..3596)
                     /locus_tag="VM910_25545"
     tRNA            complement(3512..3596)
                     /locus_tag="VM910_25545"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3560..3562),aa:Leu,seq:gag)
     gene            3830..>4396
                     /gene="lipB"
                     /locus_tag="VM910_25550"
     CDS             3830..>4396
                     /gene="lipB"
                     /locus_tag="VM910_25550"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HVI65922.1"
                     /translation="MISARDDVMVGLDRASSPVEWRISEGLVAYETALALMEARAAAI
                     ADGVASELIWLLEHPPLYTAGTSAKRAEVMEPRFPVHSAGRGGQLTYHGPGQRDVYVM
                     LDLKRRGQDARRFVATLEEWIIHALASFDVAGERRADRIGVWVRRPDKGAGREDKIAA
                     IGVRIKRWVTLHGVALNIDCDLSHYAGIV"
BASE COUNT          879 a         1308 c         1371 g          838 t
ORIGIN      
        1 ggagaattgg gcaaagcttc ccgaaggccg tgcgtgggga caggcgatcg gagtcgacat
       61 cgatcgagat ggcaccagcg tctgggttta cgatcgctgt ggcgcaaaga cctgcgaggg
      121 ctcgaatatc ccgccgatcc agaagttcga cgcctccggc aagttggtga cgaggttcgg
      181 tggcggcatg atcaactggc cgcacggtct gtttgcggac cgggacggca acgtctgggt
      241 gaccgacggc agaggcggca acggcaaggg ccacacggtc gtcaagttta gttcggatgg
      301 aaaggtcctg atgacgctgg gcaagcctgg ggtcgcgggc gatgggcccg acaccttcaa
      361 tgggccgtcc gatgtcttgg tcgcgcctaa tggcgacatc ttcgtcgccg acggacatgg
      421 cgagaagacc aacgcgcgca tcgtgaagtt ctcgaaggat ggaaaattca tcaacgcctg
      481 gggcaaagcg ggcaagggac aaggtgaatt cgacgtgccg catgctctcg ccatggattc
      541 gagcgggcgg ctgttcgtcg ctgaccgctc aaacgaccgc atccagatct tcgaccagga
      601 tggaaaattc ctggctgaat ggcggcagtt tggccggccg agcggcctct acatcgaccg
      661 caatgacatc ctctacgcgg ccgattccca gtcgggcgag aaattcaatg caccgtttcg
      721 gcagggcata aggatcggca gcgccaaaga tggcaaggtc gtagccttca tcactgagga
      781 agggccgacg cccaatatgc cggaaggtgt cgcggccgac aaagacggta acgtgttcgg
      841 cggatttacc ggcaaacaga ccctaaagaa gttcgtgaag aattagctgc cgtgtcagcg
      901 cgccggggcc gggatgcgtt ctggccccag cgcgctcgat gcaagctcct tgggttgacc
      961 cgtttacgga aagacaggca gccaatgtca gctcgtggca catcgcgtca gttcgcggcg
     1021 cagcatcaat tcggtcgctc ttggaggcat agcggacata aatgaacgct ttggcaaact
     1081 cgatcgagtt tagaccgaag cagctgtagc ggttacacct tatggccttt tcgcatggag
     1141 cgcggccacg agcggtccgg gcaaggctcg ttcagctaat gctgtaagcc gatcttcatg
     1201 acttcggatc aggtagccac atccaaaaaa gccggccaac gcgaggacaa acacgatggg
     1261 cgccagcgcg tggtgaagtt gaagcagcgc tttaccaaac atgaagccgc caagaccgaa
     1321 caccacggcc cagatgacgc cgccagtggc attggccatc agaaacacgc gccatggcat
     1381 gcgattcagt ccggcgagga atgccgccaa tatacgcagt aacgcaacga agcggccaag
     1441 gaacacaacc ttcgctccgt gcctcaagaa caggtactgt ccgagcttta tacggggttc
     1501 aaacatgcca atacgcccgc cgtatcgcag caaaagcgcg taaccgtatc ttgatccgag
     1561 ccaatagccg atgttatcgc cgatgatcgc gccaaatgcg gcgacggcaa ttaccaccca
     1621 aatgttgaag ctgggctctg tcgcagcata gatggctgca aggacgagta tcgtctcacc
     1681 gggcagaggc aatcccagac tttccaatcc caccattaga cctactaccc aatacccatg
     1741 caggctcacg agctgctgca gttctccgag gaaagtcgtg gttgctggca tcgcaggtgc
     1801 tgtcgcctga ctggacgggc ataagaactt tctaccgctc aaatagccct tcgtggcgcc
     1861 cttaccaact gcacagtgtc gcacgatgcg atacaatgtg ccgaaaccag ttgcgagcag
     1921 cgtaacgact tgctggatcg cgcccttgtc gtgctgactg ccgcgaccca ggatgcggcc
     1981 ttcttcggta ccatgaagac ttgaccaagc aatgcgcttc gcgaatcaca gcgccgccga
     2041 ccttgccagc cgccgcggca cttgcggacg ggcggctcgc ggcaccgttt tcctcaatca
     2101 gcctggcgcg acttggtgta tcggcgcgac ctgcacgccg tgatgatgga gcgcgagatg
     2161 cggcccgcgc gtgtgacgca cgccggggcg gccgacgcaa tcggcccggc acaatcgtcg
     2221 tcgcacttcg agagttcgat tgagtctgtt gttgctgctg gctgtgggcc ggacttcctc
     2281 agctgcattt ggcacgtcta tcacctatcc tacatggcgc aggtttggct gcgggtgacg
     2341 gataattacc gagacgctaa tcgagtagct gcatccttgg acgcatgacg ccgagtacgg
     2401 cgcgcgccgc gcctgttggt gtagcccatt gcggccaatt cgcgcgcaat cgaacaacta
     2461 ctttgacttt ggccagcgtc ctgctgctcg ttctcccgct ctttgggcga gccgctcgta
     2521 atcatcggca tttttgagca ttgtgcgctt ggcttcggga tcgttcatct gctccgcgag
     2581 agcgcgagct tccttagcgc ggtcaagcca gtgcttggga tcgttgattg aatagaggga
     2641 cattgaattg ctacccctct aagcggtttc acgcctatat aactcatgcc cgtgccaacg
     2701 ggttgctcac tgacttggca taaccactgg ccaccgtgag gcatgctgat accgcgagcg
     2761 ttgagagcgt cagcgatttg atggagggac gtagcgcccg gcgcgtcgca cgccgaagtg
     2821 aaacaacaac ggcgggagtt taccggaatt tttgaagtcg ggtgggcggc ccttcgttcc
     2881 aaattcgaca ggcgaaagga ggctcatttc ggaacggcgc aggcgtgatt tctcagtccg
     2941 ccgtggcgtt gcttcttgag cattgagttc ctttgaaaat gaaaagggcc gccctggttt
     3001 cagagcggga gccgaaagac tggatggccc cgggagggcg tcaccggggc catagcgctc
     3061 ccttgggctc tcaggggctc gtcaagcttc gccagtatgc cgaactgttg gcgcaagacg
     3121 tggccgcacg cgcacgtctc tttaacttcg ccagcgaggg cgagcgtttg gcggtgggtc
     3181 agagagcgcc cctttccagc tgcagcatct cgaatggctt tgatgcgggc ttcaatctcc
     3241 gccagccagt cgtggagggc tctcttacct cgccaagcgg tgtgccggca tcggcgcgaa
     3301 accgctcttc ccatcctccg ccgaagcgtt cgcgatactc atcacgcaac gtctttcggg
     3361 atgctcttgc gggcggagaa cgcacctgat ttgagccgtc tgagtgtggt catcgcaaag
     3421 gccattcgta tcgctccttc gtagcctgga ggcgaccgaa ggtctttaga tttcagtatg
     3481 ttgctgacct acaaggaaaa gtcccttgta gtggtgcggt cgagaggact cgaacctcca
     3541 cgggttgccc cgctagcacc tcaagctagt gcgtctacca attccgccac gaccgcaaat
     3601 gtaacgcggg ccgcggggcc gcgcgccgaa gcgacggcgc tcgatgtaac aaatcaaccc
     3661 ggagcgaaca agggccgcgg cgctcgctcc tccgggtcga gcgcggatgg acgcgcacgc
     3721 tgaccagccc ctgccgctgc gcagattcct tgacttccgc cgcgatgcgg ggtgttgctt
     3781 gcgtccacac ggctggagcg cttccaagcc gtcaccatat agtttcagga tgatcagcgc
     3841 gcgcgacgat gtgatggtcg ggctcgatcg ggcttcgagc ccggtggagt ggcggatcag
     3901 cgagggcctt gtggcctacg aaaccgcgct cgcgctcatg gaggcgcgcg ccgcggcgat
     3961 cgccgacggc gtcgcgagcg aactcatctg gctgctcgaa catccgccgc tctacaccgc
     4021 cgggaccagc gccaaacggg ccgaggtgat ggagccgcgc tttccggtcc attcggccgg
     4081 gcgcggcggg cagttgacct accacggccc cgggcagcgg gacgtctacg tcatgctcga
     4141 tctcaagcgc cgcggccagg acgcgcgccg cttcgtggcc acgctggagg aatggatcat
     4201 ccacgccctg gcaagcttcg acgttgcggg cgagcggcgc gcggaccgca tcggcgtctg
     4261 ggtgcggcgg ccggacaagg gcgccggccg cgaggacaag atcgcggcca tcggcgtcag
     4321 gatcaaacgc tgggtcacgc tgcacggagt ggcgctcaat atcgactgcg atctctcgca
     4381 ctatgccggc atcgtg
//
LOCUS       DATLJT010001106         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2374711,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001106 DATLJT010000000
VERSION     DATLJT010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374711"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VM910_25555"
     CDS             <1..552
                     /locus_tag="VM910_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65923.1"
                     /translation="PYPGIAVAMAQELAQKTPQQQAIASDQTVDVKDKTLGAQRQRIQ
                     VAMRPEGIRPAPTNKPSSPQSSVAPRALPFGLAAKRVSEGEILHKWTDVQSEIRDDKE
                     ILASCRESASSCPPAAQVFLAIVDEGRARSGRARIGVINRAINLAIIPTSDLVQWGVV
                     DRWSAPLETFTTRRGDCEELPNT"
     gene            528..815
                     /locus_tag="VM910_25560"
     CDS             528..815
                     /locus_tag="VM910_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65924.1"
                     /translation="MRGIAKYVALQAAGVAPEDIELVVVRNTDTSENHAVVAVRLDGT
                     WVILDNRRLALVPAREIRHATPLFVIDEQGVRQFMAPTANAQLREGIPALF"
     gene            complement(848..>2578)
                     /locus_tag="VM910_25565"
     CDS             complement(848..>2578)
                     /locus_tag="VM910_25565"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65925.1"
                     /translation="AADAALQTGFAVRGPDPRQYLVTFAGLLRNVPVIEQAAAGRGLF
                     SIDPESDGIIRRVPVIMTAQGNLVPSLSMEMLRVVTGSSAILVRVDQAGVQSVAVPGL
                     EVPTDRNGQFWVHFNHHDPERYVSAKDVLQGNAPPERLAGKLVLIGTSAIGLLDLKTT
                     PLDAAIPGVEVHAQILESVLSKSSLVNPNYAIGAELALAVLFGLAIIVAAPMLPASIV
                     IVLGGCLIAGLIGLSLYLFVEHNLLIDFTYPLISSWLIYLVLTFVNYFREQKQRRQIR
                     SAFGYYLSPHMVEQLARSPEKLVLGGEERRMTILFSDVRGFTTISEHYKDDPQGLTRL
                     MNRFLTPLTNAIIERKGTIDKYIGDAIMAFWNAPVDDEEQEANACEAALEMLLRAETL
                     NGELKREAESNGGVYMPLRIGIGLNTGPCVVGNMGSDFRFNYSVLGDTVNLASRLEGR
                     TKDYRIPVVIGSRTAEGAKRKFAVMEIDLIMVKGKKQPEAMFTVLGRSEVEADPRCQE
                     LRATNAQMLGRFRKQQWDDAIDLIARCRKFANGFDLSGLYDMYQERIELYRAQPPGSD
                     WDGVYEAETK"
BASE COUNT          532 a          783 c          769 g          494 t
ORIGIN      
        1 ccgtatccgg ggatcgctgt agcgatggcg caagagttgg cgcaaaagac accgcaacag
       61 caggcgatcg cttcggacca aaccgtcgac gttaaggaca aaacactggg cgcgcagcga
      121 caacgcatcc aggttgctat gcgtcccgag ggcatcaggc cggcgccgac aaataagccc
      181 tcttctcccc aatcctcagt tgcgccgcgg gcgcttccgt tcggactcgc ggccaaacgc
      241 gtttcagagg gagaaatctt gcacaaatgg acagatgtcc aatcggaaat tcgggatgat
      301 aaagaaatcc tggcgagctg ccgcgaaagc gcatcttcgt gtccgccggc ggcgcaagtt
      361 tttcttgcta tcgtcgatga gggccgcgcg cgcagcggtc gcgcgcgcat tggcgtgatc
      421 aatcgtgcaa tcaacttggc gataatcccg acgagcgatc tcgtgcagtg gggcgtcgtc
      481 gaccgttgga gcgcgccgct cgaaacattc acgacccgcc gcggcgattg cgaggaattg
      541 ccaaatacgt agcgctgcaa gcagccggcg tcgcaccgga agacattgag ctcgtcgtcg
      601 tccgcaacac tgacaccagc gagaatcatg cggttgtggc cgtacggctc gacggcactt
      661 gggtgatact cgacaatcgc cggctggcgc tggtgccggc ccgcgagatt aggcacgcaa
      721 ccccgctatt cgtgattgac gagcaaggcg tgcgccagtt catggcgccg acggcgaacg
      781 cacaattgcg agagggcatt ccggcattgt tctagcgcca cccccactgc ccaacggaaa
      841 ttgcaactta cttcgtctcg gcctcataga cgccatccca atccgatcct ggcggctgtg
      901 cccgataaag ttcaatgcgc tcctggtaca tgtcatagag gccggaaaga tcgaagccgt
      961 tggcgaattt gcggcagcgc gcaatcaagt ctatggcgtc atcccattgc tgcttgcgga
     1021 agcgccccag catctgcgcg ttggtggcgc gcaattcctg gcagcgcggg tcggcctcga
     1081 cttccgagcg gcccagcacc gtgaacatcg cttccggctg cttcttgccc ttcaccataa
     1141 tcaaatcgat ttccattacc gcgaattttc gcttggcgcc ctcggcggtg cgtgacccga
     1201 tgacgacggg gatgcggtaa tccttggtcc ggccttccaa ccgcgatgcc aagttcacgg
     1261 tgtcgcccag caccgaataa ttgaaacgga aatcggatcc catattgccg accacgcaag
     1321 gcccggtatt gagcccgatg ccaatgcgca gcggcatata aacgccacca ttgctttcgg
     1381 cctcgcgctt gagttcgccg ttgagcgtct cggcgcgcaa cagcatttcc agtgccgctt
     1441 cgcaggcgtt cgcttcctgt tcctcgtcgt ccaccggcgc attccaaaac gccatgatgg
     1501 cgtcgccgat atatttatcg atggtgccct tgcgctcgat gatggcgttg gtcaatggcg
     1561 tgaggaatcg attcatcagg cgcgttaatc cttgtggatc gtccttgtag tgttcagaga
     1621 tggtcgtgaa tccgcgcaca tcgctgaaca gtatcgtcat gcggcgctct tcgccgccaa
     1681 gcaccagctt ctccggcgag cgggcgagct gctccaccat atgcggggag aggtagtaac
     1741 cgaacgccga gcggatttgc cgtcgctgtt tctgctcgcg gaaataattc acgaatgtga
     1801 gaacgagata aatcagccag ctcgagatca gcggatacgt gaaatcgatg agcaggttgt
     1861 gctcgacgaa aagatagagc gacaatccga tcaggcccgc gatcaagcac ccgccgagca
     1921 cgatgacgat gctggcgggc agcatggggg cggcgacgat gatcgcaagc ccgaacaaca
     1981 ccgcaagggc gagctcggcg ccgatcgcgt agttcggatt cacgagactc gacttgctaa
     2041 gcacgctttc cagaatttgg gcgtgcactt cgacgcctgg gatcgcggcg tcgagcggcg
     2101 tcgtcttgag atccaacaat ccgatcgctg aagtgccgat caggacgagt ttcccggcca
     2161 ggcgctccgg cggagcattg ccctgcaata cgtccttggc cgagacgtag cgctccggat
     2221 cgtggtggtt gaaatgcacc cagaactgcc cgttgcgatc ggttggcact tccaggccag
     2281 ggaccgcaac cgactggacg ccggcttgat cgacacgcac caggatcgcg ctcgatccgg
     2341 tgacgacgcg caacatttcc atgctcaagg acggcaccag gttgccttgc gccgtcatga
     2401 tcaccggtac gcgcctgatg atgccatcgc tttcgggatc gattgagaaa agcccgcgac
     2461 cggcggccgc ctgctcgatc accggcacgt tgcgcaacag gccggcaaag gtcactaggt
     2521 attgacgtgg atcagggccg cgaacggcaa atccggtctg gagcgcggca tcggccgc
//
LOCUS       DATLJT010001107         4671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2144992,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001107 DATLJT010000000
VERSION     DATLJT010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144992"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VM910_25570"
     CDS             complement(<1..323)
                     /locus_tag="VM910_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HVI65926.1"
                     /translation="MDLEKFTDRARGFVQSAQSLALREGHQQITPEHLLKVLLDDPEG
                     LASGLIDRAGGRSREALSAVEAALAKLPKVSGSGAGQVYLAPALARVFDQAQEVAEKA
                     GDSYVT"
     gene            complement(663..1838)
                     /locus_tag="VM910_25575"
     CDS             complement(663..1838)
                     /locus_tag="VM910_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65927.1"
                     /translation="MIKKRLWSDRSGKNMEPAVSAHKFNDDLLLNEQDQNLAARKYPT
                     IFHILNHPELRQTFLQHDEQANRAKRNGRIAGFLAIAFGFIALAIAAVEYPVMHHAGD
                     RFVDFLRLILAGFSALCGIIGVLIGSMGVLFARRKREWLHHRLMGERIRQFHFQTLVF
                     RLPEILLSLKDDASKTVFTSNRKLWFEAIMGRLVGKLDAVLVEIIQEENISHYRLYGG
                     RSELKKDRDSKELDPLFDAYRELRILHQIGYANFKLQDDYKIFSAVPWRQAQVFTQTS
                     FVFIVALCVIDIGVLWQAFIPGSIWTAFASDSISVITIWIALAALAVRALEQGLRPER
                     EVERYQQYRSAVRAILDRFDDSESQAEKIQIMEEMERLCFDELRNFLITNERARFVL"
     gene            complement(1835..2389)
                     /locus_tag="VM910_25580"
     CDS             complement(1835..2389)
                     /locus_tag="VM910_25580"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HVI65928.1"
                     /translation="MDKQASKVGHTESTSGKVFICYRRDDVAGHAGRVHDRLQAEFGR
                     DLLFMDVDSVPLGIDFVKHLREAVAKCEVLLAVIGPNWLDLRDERGARRLDDPNDFVR
                     IEIGTALQRQIHVVPILLEGTRIPRPDQLPNDLAGLAQRQGLEIRHVSFHSDMDKLIT
                     ALRTQLAGGRTDKEPLTTPNRSRM"
     gene            complement(2384..4050)
                     /locus_tag="VM910_25585"
                     /pseudo
     CDS             complement(2384..4050)
                     /locus_tag="VM910_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            4256..>4671
                     /locus_tag="VM910_25590"
     CDS             4256..>4671
                     /locus_tag="VM910_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HVI65929.1"
                     /translation="MSLENPKIHAIYRYPVKGLSPEPLARTTLAVQDTLPADRLYAIE
                     NGPSGFDPAAPHHQPKQRYLMLMRNERLAQLRTRFDDSSHTLTVDEQGREVARGDLRT
                     PAGRAAIERFFAIFCADELRGAPKVLHAPGFSFSDVA"
BASE COUNT          937 a         1462 c         1278 g          994 t
ORIGIN      
        1 gtgacgtagc tgtcgcccgc cttttccgcg acttcctgcg cttgatcgaa tacccgcgcc
       61 agtgccggtg ccagatagac ctgaccggcg ccgctgcccg acactttggg aagcttggcg
      121 agcgccgcct cgaccgccga aagcgcctcg cgcgagcgcc cgcccgcgcg gtcgatcaag
      181 ccgctggcca agccctcggg gtcgtcgagc agaaccttga gcaggtgctc gggagtgatt
      241 tgctggtggc cctcgcgcag ggccagcgat tgggcggact gaacgaaccc gcgggcgcgg
      301 tcggtaaact tttccaagtc catgtcactt ccctcagcct gcacttccgc ccttgcggca
      361 cggaagtagc accttcaatg ggctagcttc gacggcattg atcaaagtca aagcgtagag
      421 cggatggcca atctacccac ataaattggg aactgcgggt ctattacgaa aggggtttgc
      481 agcggcggga ggctaggaac ctcgacgtcg tcactgggcg ggcaaccggc cgcccagcca
      541 acttgcgcgc tttttgatcg acatcttcga ccgtgagtgc cgcatgatgc cttgatcatg
      601 cggaagacat ccaacgccaa agacttttcc gacatcacgc ctaaacgttc ctccaagaag
      661 ttttatagaa cgaatcgagc ccgttcattg gtgataagaa agttgcgcag ctcatcgaaa
      721 cacaatcttt ccatctcttc catgatttgt atcttctcgg cctgcgactc gctgtcgtca
      781 aatcgatcaa ggatagcccg tacagctgaa cgatactgct gatagcgctc aacctcgcgt
      841 tcgggccgga gtccttgttc gagggcacgc accgcgaggg ctgcaagagc aatccagatt
      901 gtgatcacgc ttatgctgtc cgaagcaaat gcggtccaga tggaacctgg gatgaaagcc
      961 tgccacaaca ctccaatatc gatgacgcat aatgccacga tgaacacgaa gctggtctga
     1021 gtgaacactt gagcctgacg ccacggcacc gcagaaaaga tcttgtaatc atcctgtaat
     1081 ttaaaatttg catagccgat ttgatgcaag atccgaagct cacgataggc atcgaatagt
     1141 ggatcgagct ctttgctgtc tcgatctttc ttcaactcac tccgacctcc atacagccgg
     1201 taatgggata tattctcctc ctgaattatt tccacgagaa ccgcatccaa cttaccgacg
     1261 agccggccca tgattgcttc gaaccaaagc ttgcggttcg aagtgaacac agttttggat
     1321 gcatcgtctt tcaacgacaa caggatttcc ggtaatcgaa atacgagcgt ctgaaagtga
     1381 aactggcgaa ttcgttcacc catcaagcgg tgatggagcc attcccgctt ccttcgtgca
     1441 aacaggacgc ccatactgcc gatcagaact ccgattattc cgcatagggc cgagaagccg
     1501 gccaggatca accgaaggaa atcgacaaaa cgatcccccg cgtggtgcat cactgggtat
     1561 tcgaccgccg cgatggcgag cgcaatgaag ccaaacgcaa tcgccagaaa gcccgcgatc
     1621 cgaccattac gtttggcgcg gttggcttgt tcgtcatgtt gcaagaaagt ttggcgcagt
     1681 tccggatgat tcaaaatatg gaaaatcgtt ggatatttgc gagcggctaa attctgatcc
     1741 tgctcgttca gcaggaggtc atcgttgaac ttgtgagcgc tgactgctgg ctccatattc
     1801 ttgcccgatc gatctgacca aagccgcttc ttgatcacat tcttgagcga ttgggcgtcg
     1861 taagcggctc cttatcagtg cggcctcccg ccagttgggt tctcagcgca gtaatcagct
     1921 tgtccatgtc actgtggaac gatacatgac gaatttcgag gccctgacgt tgcgcgagcc
     1981 ctgcaagatc attaggcaac tgatcaggcc ttgggatccg cgtgccttca agcaaaatcg
     2041 gaacgacatg gatctgacgc tgcaaggcgg ttccgatctc gatccgaacg aagtcatttg
     2101 gatcatctag ccgacgggct ccgcgctcat cacgtaggtc aagccaattt gggccgatca
     2161 ccgcgagtaa cacctcgcac ttggctaccg cttcgcgcag atgctttacg aagtcgatgc
     2221 ccaacgggac cgagtccacg tccatgaaaa gcaaatcgcg gccaaactcg gcttgcagtc
     2281 gatcatgaac ccgtccggca tgtcccgcga catcatcgcg acggtagcag atgaaaactt
     2341 tgcctgatgt cgattcggtg tgccctactt tcgacgcctg cttgtccact tcgccactga
     2401 gaatctgatc gactgcgaca ccggtctccc gcgatatcac ccacgcaaca tggtcggccg
     2461 tcaccgcctc ctcgaccatg atgcttccgt cttccgctgc cacgatgctc ttgagcttcc
     2521 tctcgatctc tgggattcgg ccgtaggcga gctcgcccgc gcgctggtag tcacctttgc
     2581 gttgggcatt cgcgagctcg acccgtaaat gatcgagctc ggtcttgagc ttgtgggtct
     2641 ccgaaacccg attcttctcg gccttccacc gtgccgtcag attggcggag tgcttttcga
     2701 gcgcgaccaa ctccttctcg agccgcttga gccgctcctt cgaacctgga tcgctctctc
     2761 tcctgagtgc ctcttgctcc atcttgagcc gcacgatctc acggtcgatc gaatccagcg
     2821 cctcaggttt ggaatcaact tgtatcttga gccgcgcggc cgcctcatcg atcaagtcga
     2881 tcgccttatc cggcaggaaa cgattgctag cgtaacgatg acgggtggac agagtcgctg
     2941 ccgcgaccaa tgccgaatcc gtaatccgga tgccataatg ctgctcgtag cgatgtttca
     3001 agcctcgcag gatcgaaatc gtttcttcga ccgtgggctc cgatacaaat atcgcctgca
     3061 acgacgggca aaggctgcat ctttctcaat atacctcttg tatccttcca gcgtagcaac
     3121 tccaatgcac tggagatcct cgttatcgat tcctgtcttc agcagatttg aggccgcgac
     3181 tgcgttctct gtaccgctcc cgatgaggct gtgcatctca tcgatgaaca ggatgatgcc
     3241 gccctcggac gcggtcacct cctgcagcac agcctttagc cgctcctcga actcgccgcg
     3301 atacttggca ccggcgatca gcgcgcccat gtcgagcgcc acgatcttcc tatcctctaa
     3361 gttgtctggc acatcgccag agatgatgcg cagaaccacg cctttgacta ttgctgtctt
     3421 accgacgcct ggctcgccga tcaggaccgg attgttcttg gtgcggcggg agagcacctg
     3481 gatggtgcgc cgtatctcct catcgcggcc gatcaccgga tcgatcttgc cgtggcgtgc
     3541 ggcctgggtg aggtcgcgcg tatatttctt cagcgcgtcg taggcgttct cagccgggcg
     3601 cgggtcgacc gtgcggccct tgcgcaacgc gttgatcgcc gcgttgaggc tttgcggcgt
     3661 cacccccgcg gcggcgagta tccgtcccgc ctcgctgttc ttctcgagcg cgagcgcgag
     3721 cagcagccgc tcgacggtga cgtagctgtc gcccgccttt tccgcgactt tctgcgcttg
     3781 atgcgccagc gccggcgcca ggtagacctg accagcgccg ctgcccgata ccttgggaag
     3841 cttggcgagc gccgcctcca cggccgaaag cgcctcgcgc gagcggccgc ccgcgcggtc
     3901 gatcaagccg ctggccaagc cctcggggtc gtcgagcaga accttgagca ggtgctcggg
     3961 cgtgaactgc tggtggccct cgcgcagagc cagcgattgg gcagactgaa caaacccgcg
     4021 ggcacggtcg gtgtatttct cgaaatccat gtcagccccc cgcggcatgc ctcgggcgcc
     4081 tgcggcgcgc gagggggtct ccatcgccgc tgacctaagc caaatacctt cgctgaatca
     4141 atccgcgcaa gctccaaata gcaattttca gaggcggccc tttgcggcgc agaggctgcc
     4201 acccatttga atgccgtcta ggcaggcggt ggttcaccgg gttaacaacc acgccatgag
     4261 cctcgaaaat ccaaaaatcc acgcgatcta tcgctatccg gtcaaggggc tttcccctga
     4321 gccgctcgcg cgcacgacgc ttgcggtcca ggacacgctg ccggccgatc gcctctacgc
     4381 catcgagaac gggccgtccg gtttcgaccc cgctgcgccc caccatcagc ccaagcagcg
     4441 ctacctgatg ctgatgcgca acgagcggct ggcgcagctg cgcacacgct tcgacgactc
     4501 gagccacacg ctcaccgtcg atgagcaagg ccgtgaggtc gcgcgcggcg acttgcgcac
     4561 gcctgcaggc cgcgccgcga tcgaacggtt cttcgcgatc ttctgcgccg acgagttgcg
     4621 aggcgcgccg aaagtgctgc acgcacccgg cttcagcttc tccgacgtgg c
//
LOCUS       DATLJT010001108         5095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1916325,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001108 DATLJT010000000
VERSION     DATLJT010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916325"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VM910_25595"
     CDS             complement(<1..396)
                     /locus_tag="VM910_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65930.1"
                     /translation="MIRVRILQLGLVCAAIVALELLCRLGIIDRFTMIPPSEMTRALA
                     HVVATAPWFWPDVAYTLGNLVAAIGLSVLGGFLVGVAVHAMPRLRRVLDPIFTSYYAV
                     PTFVLYPLLIVFFGIGPTSLIVLGALFGIV"
     gene            complement(470..1429)
                     /locus_tag="VM910_25600"
     CDS             complement(470..1429)
                     /locus_tag="VM910_25600"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI65931.1"
                     /translation="MWFRATLATVVMSLAVTPACAAKITVSQYGRLVATLPWAVALQK
                     GMFKEAGLDIDGITAGAGGGTSLRNMLAGELPYAELATSAAIAGSLAGVELKALASSS
                     NHIGELTWAAKPDAGINSIKDLVGKKVAYTSPKSITEMVIRTALKQEGLAGKVDILPL
                     GGLGPALTALAQGAVAAAPLNDPALTVTPDKYKVLFYGHQYYPKFTWQVAVTTKEFAA
                     KNSQTLRKILNVHRQAVEFVYNNREEAARIYAKVWEVSLAEANAILPKYYEWQHWSRG
                     DFSKEGLAAVTDGLISVGELKEPFDWDKLIDQSLLDEDLRRPL"
     gene            1557..2420
                     /locus_tag="VM910_25605"
     CDS             1557..2420
                     /locus_tag="VM910_25605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65932.1"
                     /translation="MRIVHGDQIEEKIRIHQHRQGMFRYRTVAAGEPGTPGNFILEMV
                     RTTDDFFSPRHKHNFDQFRYQLEGEFDFDRNGKMTPGIIGYFPEGTPYGPQSSSVSSL
                     TLVLQFGGASGNGYMTQEQMEAGTAELKKHGTFEKGVFRRNEGEDGKRNVDGYQAVWE
                     HVNNRPMTYPAPRYHEPIMMSPDHFDWVPVEGVPGVCEKLMGVFTERSCEARFLRLAP
                     QARMPAPGRKVYFVLTGDGRVGGQSYRHYTTVFCQRGEETAFEAETPTEILVFGLPKL
                     DTSAHFAIAAE"
     gene            complement(2548..4767)
                     /locus_tag="VM910_25610"
     CDS             complement(2548..4767)
                     /locus_tag="VM910_25610"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI65933.1"
                     /translation="MNKQTNQVSVAATVSRRGFVEGAAGLMFAFTLGGLGRVGDALGA
                     TQAARINAWVAIGTDDTVTILCPSAEMGQGVMTSLPLILAEELDADWSKVKTEFAPAN
                     PKIYGNPHELFKGAQITAASVSVPGYFMPLRVAGAQARRVLIESVADEWKVPVSELST
                     DKGFVVHAQSGRRISYGDVAKFASVPPEPPKITAADLKKPAQFKLIGRKDIGRVDVPL
                     KVNGTAKYGIDVQVPDMVYASVLEAPVEGAKVEVLNLGDVTKIKGVTKVIPLPFGVAV
                     IGDTVEATRAGRNALKVKWDTTGAAAGNTDSEKAKEDYARNGKDPNAKTMVEYEVGDA
                     AAALSSAAKVVEATYWSEYCYHAQMEPMNCVAKVSEDGQSAEIWTGTQFGALAANIIS
                     GILKTTPDKIKVNQQMLGGGYGRRIWPDAAIQATILSNITKKPVKLILTREDDVAAAR
                     PRPMTHHVLKAGLDANGNLAGWHHRLVSENVDAVAAPPRFEATGGKDYIGARGLDQAF
                     YGIRNVRAEYVRETRGMRVHAWRGIGSGYNKFAAESFVDEVANAAGKDPLAMRLELTK
                     DQPRAQAVIKAVVEMSDFKRKRPGRGMGIAFSDYHGTLSAGVAEISLDRNTGKIKVHN
                     YWVAVDPGLVIQPDHVHGQLESAVVFGLSAALLEELTVKAGAIQQSNFNDYPVLRMSD
                     VPDIHTKIVTTENPPTGMGEIGVVAVAPAIANALFQLTGKRIRNLPMSPDRVKRALA"
     gene            complement(4771..>5095)
                     /locus_tag="VM910_25615"
     CDS             complement(4771..>5095)
                     /locus_tag="VM910_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI65934.1"
                     /translation="GACTVHIDGKAVRSCQTQMSTVGGKKVTTIEGLSPSSSHALQKA
                     WIVEQVPQCGYCQSGQIMQAAELLAKNKKPTRKQIIAHMDGNICRCGTYPRIVRAIER
                     AAREG"
BASE COUNT          938 a         1629 c         1575 g          953 t
ORIGIN      
        1 gacgatcccg aacagtgcgc ccaaaacaat cagcgacgtc ggaccgatgc cgaaaaaaac
       61 gatcagcagc ggataaagca cgaaggtggg aaccgcgtaa taggaggtga aaatcggatc
      121 gagcacgcga cgaagacgcg gcatggcatg caccgcgact ccaaccagaa aaccgcccaa
      181 tacggacaag ccgatcgcgg cgacgagatt gccgagggtg taggcgacgt cgggccagaa
      241 ccagggcgcg gtcgcgacga cgtgtgccag cgcacgcgtc atctcggacg gcgggatcat
      301 ggtgaagcga tcgatgatcc caaggcgaca gagcagttcg agcgcaacga tggcggcaca
      361 aacgaggccg agctgaagga tgcgcacgcg gatcatcaca cgcccagcgc cttcttcgat
      421 tcctcgcgta gcagcgccca aagccgactg gcgagcgcag ccttcgccat cagagcgggc
      481 ggcgcaagtc ttcgtcgagc aggctttgat cgatcagctt gtcccagtcg aacggctcct
      541 tgagctcgcc gacggagatg aggccgtcgg tgacggcggc cagtccttcc ttggaaaaat
      601 cgccgcggct ccaatgctgc cattcgtagt atttcggcag gatggcgttg gcttccgcca
      661 gggacacctc ccacaccttg gcatagatgc gcgccgcctc ctcgcggttg ttgtagacga
      721 actcgaccgc ctgacgatgg acattgagaa tcttgcgcag ggtctgcgaa ttcttcgccg
      781 cgaactcctt ggtcgtcacc gcaacctgcc aggtgaattt cggatagtat tgatggccgt
      841 agaacagcac tttgtattta tccggggtga cggtgagcgc cggatcgttc aagggtgcgg
      901 ccgcgaccgc gccctgcgcc agcgccgtca aggccggtcc caggccgccg agcggcagga
      961 tgtcgacctt gccagcgagg ccctcttgct tgagcgcggt gcggatcacc atctcggtga
     1021 tcgacttcgg actagtatag gcgactttct tgccaacaag gtccttgatc gaattgatcc
     1081 cggcatcggg cttggcggcc caggtcagct cgccgatgtg gttcgaggac gacgccagcg
     1141 ctttgagctc gacgcctgca agcgagcccg cgattgccgc cgaggtcgcg agttcggcat
     1201 agggtaattc gccggcgagc atattgcgta acgacgtccc gccgcccgcg cccgcggtga
     1261 tgccatcgat gtcgaggccc gcctccttga acatgccttt ttgcagcgcc accgcccaag
     1321 gcagcgtcgc caccaggcgt ccgtactggc tcaccgtgat tttcgccgcg caggcgggcg
     1381 tcacagccaa gctcatcacg acggtcgcga gcgtggcgcg gaaccacatg atgtttcccc
     1441 cgtttacgcc tgcgttgatc gcaggggtcg ttgtgtccga aagccttgta cattaggctt
     1501 tccgccaccc gcaaggtcgc cgcgcgcctc tgcaaaagac agaccggagg aaccccatga
     1561 gaatcgtgca cggagatcag atcgaggaga agatccgcat ccaccaacac cgccagggca
     1621 tgttccgcta tcgcacggtg gcagcgggcg agcccgggac gcccggtaat ttcatcctgg
     1681 aaatggtccg cacgaccgac gatttctttt ctcctcgcca caagcacaat ttcgatcagt
     1741 tccgctatca gctcgagggc gagttcgact tcgatcgcaa tggaaagatg acgcccggca
     1801 tcatcggata ctttccggag ggcaccccct atggaccgca gagctcgagc gtgagttcgc
     1861 tgacgctggt gctgcagttc ggcggcgcga gcggcaatgg ctacatgacc caggagcaga
     1921 tggaggccgg caccgccgaa ctcaagaagc acggcacgtt cgagaagggc gtctttcgcc
     1981 gcaacgaggg cgaggacggc aaacgcaacg tcgatggcta tcaggcggta tgggagcacg
     2041 tgaacaatcg gccgatgact tatccggccc cgcgctacca tgagcccatc atgatgagcc
     2101 ccgatcattt cgactgggtg ccggtcgagg gagtgcccgg cgtgtgcgaa aagctgatgg
     2161 gcgtcttcac cgagagaagc tgcgaggcac gctttctgcg tcttgcgccg caagccagga
     2221 tgccggcacc cggacgcaag gtttatttcg tgctcaccgg cgacggacgg gtcggcggcc
     2281 aatcctatcg ccactacacc acggtgttct gccaacgggg cgaggaaaca gccttcgagg
     2341 ccgagacgcc gaccgagatt ctcgttttcg ggctacccaa actcgacacg tcggcgcact
     2401 tcgccatcgc ggcggaataa ctccgaccgc atgcgctcag gactgcgagg catcagcgcc
     2461 cgcgccacca agtgcgggtc tggccagccg ttaaaaaaaa cgccgcgtcg atgtcccaac
     2521 gcggcgcgct tcgttgggct agcgcggcta tgccagcgcc ctcttcaccc ggtccggcga
     2581 catcggcaga ttgcggatcc gcttgccggt aagctggaac agcgcattgg cgatcgccgg
     2641 ggcgaccgcg accacgccga tctcgcccat gccggtcggc ggattctcgg tcgtcacgat
     2701 cttggtgtgg atgtctggca catcgctcat gcgcagcacc ggatagtcgt tgaagttgct
     2761 ctgctggatg gcgccggcct tgaccgttag ttcttcaagc agcgcagcac tcaggccgaa
     2821 gacgacggcg ctctcgagtt ggccatggac gtgatccggc tgaatgacga gacccggatc
     2881 gaccgctacc caataattgt gcactttgat cttgccggtg ttgcgatcaa gggagatctc
     2941 cgccaccccc gccgacagcg tgccgtggta gtcggagaac gcgatgccca tgccccggcc
     3001 cggacgcttg cgcttgaagt cggacatctc gaccaccgcc ttgatgaccg cctgagcccg
     3061 cggttggtct ttcgtcagtt cgagccgcat ggcgagcgga tccttgcccg ccgcgttggc
     3121 gacctcatcc acaaacgatt cggccgcgaa cttgttgtag cccgagccga tcccgcgcca
     3181 ggcgtgaacg cgcatgccgc gggtttcacg cacgtactcg gcccgcacat tgcggatgcc
     3241 gtagaacgcc tgatcgagcc cgcgcgcgcc gatgtagtcc ttgccacccg tcgcctcgaa
     3301 acgcgggggc gccgcgaccg catcaacgtt ctcggacact aggcggtggt gccaaccggc
     3361 gaggttgccg ttcgcatcga gaccagcctt gagcacgtga tgggtcatcg gccgcgggcg
     3421 ggcggcggca acgtcgtcct cgcgggtgag aatgagcttc accggcttct tggtgatgtt
     3481 agacagaatg gtcgcctgga tcgcggcgtc cggccaaata cgccggccgt agccgccgcc
     3541 caacatttgc tggttgacct tgatcttgtc cggcgtggtc ttgaggatgc cggagataat
     3601 gttggccgcg agcgcgccaa attgcgtgcc ggtccagatt tcggccgatt gaccgtcctc
     3661 cgagaccttg gccacgcagt tcatcggctc catctgcgcg tgatagcaat attccgacca
     3721 ataagtggct tctaccacct tcgcggcgct gcttaacgcc gcggcggcat cgccgacctc
     3781 gtactccacc atggtcttgg cgttggggtc cttgccgttg cgagcatagt cttccttggc
     3841 cttttcggag tccgtgttac cggcggcagc gccggtcgta tcccacttga ccttgagagc
     3901 gttgcgtccg gccctggtcg cctcgaccgt atcgccgatg acggcaacgc caaacggtag
     3961 cggaatcacc ttggtcacac ccttgatctt tgtgacgtcg cccaagttga ggacttcgac
     4021 cttggcgccc tcgactggag cttccaacac cgaggcgtag accatgtccg gaacctgcac
     4081 gtcgattccg tacttggcgg ttccgttgac tttcagcgga acgtcgacgc ggccgatgtc
     4141 cttgcggccg atcagcttga actgtgccgg tttcttcaaa tccgccgccg tgatcttcgg
     4201 cggctcgggg ggcacgctgg cgaacttggc gacgtcaccg taggagatgc ggcgacccga
     4261 ctgcgcgtgg acgacgaagc ccttgtccgt cgacagctca ctcaccggca ctttccactc
     4321 atcggccacg ctctcgatca gcacgcggcg tgcctgtgcg ccggctacac gcagcggcat
     4381 gaaatagccg gggacggaga cgcttgcggc ggtaatctgc gcgcctttga acaactcgtg
     4441 cggatttccg tagatcttcg gattcgcggg cgcgaactcg gtcttcacct tcgaccagtc
     4501 ggcgtcgagt tcctcggcca gaatgagcgg caaggacgtc attacgccct gacccatttc
     4561 cgccgacggg cagaggatcg tcaccgtatc gtcggtgccg atggcaaccc aggcattgat
     4621 cctggcagct tgcgtggcgc ccagcgcatc gcccacgcgg cccaagccgc caagcgtgaa
     4681 ggcaaacatc agcccggcgg cgccttcaac aaagccgcgc cggctcacag tcgcagcgac
     4741 tgatacttgg ttggtttgct tgttcatggc tcagccctcc cgagcggcgc gctcgatggc
     4801 gcgaacaatg cgcggatagg tgccgcagcg acaaatgttg ccgtccatgt gggcgatgat
     4861 ctgcttgcgc gtcggctttt tgttcttcgc caacagttcc gccgcctgca tgatctgacc
     4921 ggactggcag tagccgcact gcggcacctg ctcgacgatc caggccttct gcagcgcgtg
     4981 actggagctc ggcgacagcc cttcgatggt cgtgaccttc ttgccgccca cggtcgacat
     5041 ctgggtctgg caggatctca ccgccttgcc gtcgatatgg accgtgcagg cgccg
//
LOCUS       DATLJT010001109         2485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3754347,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001109 DATLJT010000000
VERSION     DATLJT010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3754347"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VM910_25620"
     CDS             <1..1139
                     /locus_tag="VM910_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HVI65935.1"
                     /translation="GIRSERRLCEEVHLNLAYRWFCRLGLEGDVPDHSTFSKNRHGRF
                     RDSDLLRRMFETVLQRCMREGLVGGEAFAVDASLIRADANRQKGIEGEKGLPAEATGR
                     AVEEYLAVLDDAAFGAATEVTPKFISPADPAARWTGAHGGQAFFGYSTNYLIDAENAI
                     IVDVEATTAIRQAEILAAKRMIERSMERFDLYPAKLMGDSAYGSAEMIGWLVYEHGIE
                     PHVTVFDKSERRDGTFSRDDFTYDHAIDVYVCPGGKLLTTTGSLIDGATLRYRASKYD
                     CQACRLKPRCCPKEPARYVPRSIYEGGRDMAREIAKSWEGRTSRRLRKKIEMLFAHLK
                     RILKLDRLRLRGPNGARDEFILAATAQNLRKMAKLIPMPTLRPA"
     gene            complement(1426..>2485)
                     /locus_tag="VM910_25625"
     CDS             complement(1426..>2485)
                     /locus_tag="VM910_25625"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI65936.1"
                     /translation="GSGNFKEYTKMWNNEITRQLGIKLPFVGAGMAIVGSPALTAAVS
                     NAGGIGLFCLGPGTPEMLAGGIDSIRRLSSAPFGVDFIVEETGFGPATTEAHVEVAIA
                     KRVPLAVFFWNPPEERWISELKRAGIKVWGTAYSVESARRLKALGADAVIVQSHEAGG
                     HVRSDLGAMSLLPAVVDALERTPIIAAGGIADGRTAAAAFMLGAQAVCVGTRLLASDE
                     SLASAEYKERLVRAHGDDTVVTTIFGPEWPDAPMRVLKNSAVARSEQGNMPPKQLIGE
                     TVVFGRSYKMPANSAVLPTTQTRGDHEEMCLAAGASVAAVRSIEPADQIVRDMMTDAW
                     ALIGGNNVKYPPETGRNR"
BASE COUNT          504 a          778 c          708 g          495 t
ORIGIN      
        1 tcggcatccg ttcggagcgg cgcttgtgcg aagaggttca cctcaatctg gcataccgct
       61 ggttctgccg ccttgggctc gagggtgacg tgcccgacca ttcgaccttc tcgaagaaca
      121 ggcatggccg cttccgcgac agtgatctgc tgcgacgcat gttcgagacc gtgctgcaga
      181 gatgcatgcg agaaggtctg gtcggcggtg aggcgttcgc agtcgacgcc agcctaatca
      241 gggcagatgc caataggcag aaaggcatcg aaggcgaaaa ggggctgccg gcggaagcga
      301 ccggccgagc agtcgaagaa tatctagctg ttcttgatga tgcagccttc ggcgctgcga
      361 ccgaagtgac ccccaagttc atctcaccgg ctgatccggc ggcgcgctgg accggcgccc
      421 atggcgggca agccttcttt ggctactcga ccaactatct gatcgacgcc gagaacgcga
      481 tcatcgttga tgtcgaagca accacggcga tccgacaggc cgagatcctc gccgccaaac
      541 gcatgatcga acgttcgatg gagcgcttcg acctctatcc tgccaaactc atgggcgata
      601 gcgcctacgg ctcggctgag atgatcggct ggctggtcta cgagcatggc atcgagccgc
      661 atgtgacggt attcgacaag tcagagcgcc gggatggtac cttctcacgc gacgacttca
      721 cctacgacca tgccattgat gtctacgtct gccccggcgg caagttgctg acgacgaccg
      781 gctcactgat tgacggtgcg acactacgct accgggcgag caagtatgat tgccaagcct
      841 gccgtctaaa gccccggtgc tgtccaaagg agccggctcg atacgtgccg cgatcgatct
      901 atgaaggcgg ccgtgacatg gcgcgcgaaa tcgcaaaatc gtgggaggga cgcacctcgc
      961 gccggctccg caagaagatc gagatgctgt tcgcgcacct caagcgcatt ctcaagctcg
     1021 accgattacg cttacgaggg ccaaacggcg cgcgcgacga gtttatcctc gcagcgaccg
     1081 cccagaacct tcggaagatg gccaagctga tcccgatgcc caccttgagg cccgcatagg
     1141 aacggcaaag ccggtgttcg cgccattaca atgaattact gccggtcttt ttcaacggaa
     1201 tcggccaaga ggcgacattc gcagggatat gaaagaggac cgccaactga ggcggcccta
     1261 cttcaacttc ttggtgctga tacggatttg aaagagtggg gtccgagttt cgtcacctct
     1321 agcgcccatt cctgatcggg gcacatccta tcatcaatat ccttgaatat ttcagcggca
     1381 aagattgtgg cctcgcgcca tgccacatca tcgctcggta gttcttcacc gatttcgtcc
     1441 agtttcgggg ggatatttca cattgtttcc tccgatgagc gcccaggcgt ctgtcatcat
     1501 gtctcgcacg atctggtccg caggctcgat cgagcggaca gctgcaacgc tggcacctgc
     1561 tgcgaggcac atctcctcgt ggtcacctcg cgtttgcgtg gtcggcaaca ctgcgctgtt
     1621 ggccggcatt ttgtaagatc gcccgaacac gaccgtctcg ccgatcagct gtttcggggg
     1681 catgtttccc tgctcggagc gagcgacagc actatttttt agaacccgca tcggcgcgtc
     1741 aggccattca ggaccaaaga tcgtcgtcac gactgtgtcg tctccgtgag ctcgcacgag
     1801 gcgctccttg tactcggcgc tcgccaagct ctcgtcgctc gcaagaagcc gcgtaccgac
     1861 gcagacggcc tgggcgccaa gcatgaacgc ggccgcagct gttcggccat ctgcgattcc
     1921 tcctgccgca ataatcggcg ttcgctccag cgcatccacg accgcaggca aaagcgacat
     1981 cgcgccgaga tcggacctca cgtggccgcc tgcttcgtgt gactgtacaa taaccgcatc
     2041 tgcgccgagc gccttaagcc gccgtgcgct ctcaaccgag taagccgttc cccatacctt
     2101 gatcccagca cgcttcagct cgctgatcca acgctcctct ggtggattcc agaagaagac
     2161 cgcgagcggg acacgcttgg ctatcgcgac ctcgacatgg gcttctgtcg tcgcaggacc
     2221 aaagccggtc tcttcaacga tgaagtctac accaaaaggt gctgagctca ggcggcgaat
     2281 gctgtcgatc ccgccggcga gcatctccgg cgttcccggc ccgaggcaaa ataggccgat
     2341 gccgcccgca ttcgacaccg ctgccgtgag cgcgggactg ccaacgatcg ccatgccagc
     2401 cccgacaaac ggcaacttga ttccgagttg tcgcgtaatc tcattgttcc acattttcgt
     2461 gtactccttg aaatttccgg agcca
//
LOCUS       DATLJT010001110         5987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2145199,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001110 DATLJT010000000
VERSION     DATLJT010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145199"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VM910_25630"
     CDS             complement(<1..267)
                     /locus_tag="VM910_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI65937.1"
                     /translation="MTKDRALEASKNGLVASRTLSKRQSRRKFLKAAGSALAAGAFGA
                     VPLRGWAADPVNIGALYPTTGGMAQIGVGCVAAAKLAVEMINDAG"
     gene            449..1192
                     /locus_tag="VM910_25635"
     CDS             449..1192
                     /locus_tag="VM910_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI65938.1"
                     /translation="MAAKLFKGVKVRQSRETARVPARAPAAAVLIARSIEEDIVLGRR
                     QPRERLVEQDLSDLFHTHRGDVRLALFELEKKGLVERIPNRGAMVRGLTPSEVKEIYA
                     VREEVEVMAVRIIPFPVSTADIARLEELQRQHAKAIANCEMLTVFYSNLRFHQALFGL
                     CGNACLIETIDLLAQKVYGIRSYANAFPEQLDRARRDHLDMIRALRDSQREELIALTR
                     RHLKPSPQAYIQAYERRFGRAEAASTAAR"
     gene            1337..2491
                     /locus_tag="VM910_25640"
     CDS             1337..2491
                     /locus_tag="VM910_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI65939.1"
                     /translation="MQNDRQPQSIRHLSKSLPGQVVLVLQGGGALGSYQAGVYQALHE
                     EAIAPDWIIGTSIGAINASLIAGNEPGQRLARLKEFWKRMEQTSVWHVSDAFAGFHER
                     LSAWSTIAQGIAGFFKPNPLAHIGDSYPLGADHAGYYSIAPLEQTLLELVDFDLVNRG
                     SPRLTVGAAHVRTSEMRYFDSLRCALSVKHVLASGALPPAFPAVRIEGELYWDGGVLS
                     NTPTEAVFDDNPRKDSLIFSVHLWNPFGAEPTTMGEVFNRQKDVQYSSRIATQIVRQQ
                     QIHRLRHVINELAARLPHAELNKPLVRELAGYGCQTRMHVVRLQAPQLDRETHTKDID
                     FSAAGIKRRWDAGYAHTKAALERAPWDGEFDPLSGVILHEGPEPLPRAAE"
     gene            complement(2507..3577)
                     /locus_tag="VM910_25645"
     CDS             complement(2507..3577)
                     /locus_tag="VM910_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVI65940.1"
                     /translation="MRIAQVAPLTEAVPPKLYGGTERVVHWLTEELVALGHEVTLFAS
                     GDSHTSAKLEATWPKALRLDGSVRDPNALHMVMLERVRRKCDDDEFDFLHFHLDYYPF
                     SLFCRQPTPFLTTLHGRLDLPEHQPVFNTFNEIPVVSISNTQRRPVPHARWVRTIHHG
                     LPESLLMPKSSSQGYLAVLGRIAPEKGVDRAIRIATRCGIPLKIAAKIDRADQDYYDH
                     VIAPLINSNRLVDFIGEINDHEKSDFLSGAIGLLVPIDWPEPFGLVMIEAMACGTPVI
                     AYNRGSVPEIVEDGVTGFIVEDETSAVASVDRLSALSRSVIRQQFEARFTARRMALDY
                     LAAYRSLMEAAEPRIKLVSSAE"
     gene            3882..5645
                     /locus_tag="VM910_25650"
     CDS             3882..5645
                     /locus_tag="VM910_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65941.1"
                     /translation="MDQLSGYAHRPLHFVLRYLRRRPVSHLVILTAVVAAVACSVGTQ
                     YGVKYLVDSLAAGPGLATHVWLAFGLLMSLIAADNFLWRIASWTASFTFVGVTGDLRR
                     DMFRHLTGHAPSYFSDRLPGMLTSRITATSNAVFTVENMFVWNVLPPCIATVAAIALI
                     GTVSLSMSAALIVLAGTMVVAMFRLAAAGKPLHDDFADKAAIVDGEMVDVISNMPLVR
                     AFCGLSHEHARFDATVGRELIARGRSLRYLEKLRILHAGVTLVLTIALLAWAIRLWQQ
                     GGATTGDVVLVCTLGLSILNATRDLAVALVDVTQHVARLTEAIATLLLPHELRDHPEA
                     EPLMRSGAAIAFDNISFQYPGGAKVFDRFNLRLQPGQRVGLVGHSGGGKSTLFVLLQR
                     FYDVDQGHIIIDGQDISRVTQQSLREAISVVPQDISLFHRSILENIRYGRPNATDDEV
                     LRAAIAARCDFVETLPAGLDTMVGDRGVKLSGGQRQRIAIARAFLKDSPILLLDEATA
                     ALDAESEEAIREALGRLMRGRTVIAIAHRLATLRSFDRLVVLRSGKILEDGPPDRLMQ
                     VQGPYRELVTQEMSRLAKFAA"
     gene            5710..>5987
                     /locus_tag="VM910_25655"
     CDS             5710..>5987
                     /locus_tag="VM910_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HVI65942.1"
                     /translation="MTSEAVSQVLATSRTYEVVVEQPFYIPMTGPAARLRRSLKHDDT
                     FIVLDSHGDIGASAGGPDGLFHADTRYLARLQLLFDDLQPLLLGSNLR"
BASE COUNT         1050 a         1890 c         1906 g         1141 t
ORIGIN      
        1 gccggcatcg ttgatcatct cgaccgcaag cttggcggcg gcgacgcagc cgacgccgat
       61 ctgcgccatg ccgccggtcg tcggataaag cgcgccaatg ttaacgggat cggccgccca
      121 gccgcgcagc ggcaccgcac cgaaggcgcc tgccgccagc gcagaaccgg ctgccttcag
      181 gaatttgcgg cggctctgcc gtttcgacag agtgcggctt gcaacgagcc cgtttttaga
      241 cgcctcgagc gcgcgatcct tggtcatgcc gtctcccctg attgcggtcc agccgcgacg
      301 cattgcgccc gcccgtccgc ctgtttgttc ggtgcggatg ctagcgcggg tgaaaattgt
      361 ttacaatata gttttcaata ctatcattgt ggaagtttcg ccggtcctgg gccaagcgcc
      421 tgtgccggcg gtcttgcgat gggacttgat ggcggcaaag ctgttcaaag gcgtgaaggt
      481 gcgacaatcc cgcgagacgg cgcgcgtgcc ggcgcgcgct ccggcggcag ccgtcctcat
      541 cgcgcggagc atcgaagaag acatcgtgct tggccggcgg cagccgcgcg agcggctggt
      601 cgagcaggat ctctccgacc tctttcacac ccatcgcggc gatgtgcggc tcgcgctgtt
      661 cgagttggag aagaagggtc tcgtggaacg gattccgaac cgcggcgcga tggtccgcgg
      721 actgacgccg tcggaggtga aggagattta cgcggtgcgc gaggaagtcg aggtgatggc
      781 ggtgcgcatc atcccgtttc cggtttcgac agccgatatc gcgcggctcg aggagctgca
      841 gcgccagcac gcgaaggcga tcgcaaactg cgagatgctg accgtattct acagcaatct
      901 gcgcttccac caggcgctgt tcggcctctg cggcaatgcc tgcctgatcg agaccatcga
      961 tttgcttgcg cagaaggtct atggcatccg ctcctacgcc aatgcttttc ccgaacagct
     1021 cgatcgggcg cggcgcgacc atctcgacat gatcagggca ttgcgcgact cgcagcgcga
     1081 ggaattgatc gcgctgacaa ggcggcattt gaaaccctcg ccgcaggcct atattcaggc
     1141 ctatgaacgg cgcttcggca gagccgaggc ggcaagcacg gccgcacgct agcatgagtg
     1201 caccgcgccg gtcggtgcgt cggtaagcgc attgaaatgt cgcttccgac gtaacaaaat
     1261 ccgccccgat cgcgaatact gacttgcaat caaccagggg acgcgatcaa ggggcatcca
     1321 agcaggaagg aaccgtgtgc agaacgatcg tcaaccgcag agcatcaggc acctcagcaa
     1381 gtcgcttccc ggccaggtcg tgctggtgtt gcaaggtggc ggcgcgctcg gctcctatca
     1441 ggccggggtc tatcaggcgc tgcatgagga ggccatcgcg cccgattgga tcatcggcac
     1501 ctcgatcggc gccatcaacg cgagcttgat tgccggcaat gaacccgggc agcggctcgc
     1561 gcggctgaag gagttttgga agcggatgga gcagacgtcg gtctggcatg tcagcgatgc
     1621 ctttgccggc ttccacgaac ggctctcggc ctggtcgacg atcgcgcagg ggattgccgg
     1681 cttcttcaag cccaatccgc tggcccatat cggcgattcc tatccgctcg gtgcggatca
     1741 cgccggctat tattcgattg cgccgctgga gcagacgctt cttgagctcg tcgatttcga
     1801 tctggtcaat cgcggcagcc cgaggctgac ggtgggtgcc gcccatgtgc ggaccagcga
     1861 gatgcgctat ttcgacagcc ttcgttgcgc actttcggtc aagcatgtcc tggcatcggg
     1921 ggcgctgccg ccggcctttc cggcggtgcg gatcgaaggc gagctctact gggacggcgg
     1981 cgtgctgtcg aacacgccga ccgaagcggt gttcgacgac aacccgcgca aggactcgct
     2041 gatcttctcc gtccacctct ggaatccgtt tggcgccgag ccgactacca tgggcgaagt
     2101 gttcaaccgg cagaaggacg tgcagtattc cagccggatc gcgacccaga tcgtgcgcca
     2161 gcagcagatt catcgcctgc gtcatgtcat caacgagctt gctgcaaggc tgccccacgc
     2221 cgaactcaac aagccgcttg tccgcgaact ggcgggctat ggttgtcaga cgcgaatgca
     2281 tgtggtgcgc ctgcaggcgc cgcaactcga tcgcgagacc cacaccaagg acatcgattt
     2341 cagcgcggcc ggcatcaagc ggcgctggga tgcgggctac gctcatacca aggcggcgct
     2401 cgaacgcgcg ccgtgggacg gcgagttcga tccgctctcg ggcgtgatcc ttcatgaggg
     2461 ccccgagccg ctgccgcgcg ctgcagagtg accgctcgga tccccgctat tcggcgctgc
     2521 tgacgagctt gatccgcggt tcggccgctt ccatcaggct gcgataggcg gcgaggtaat
     2581 cgagcgccat gcgccgcgca gtgaagcggg cctcgaactg ctgccggatc acggatcgac
     2641 tgagcgccga aagccgatcg accgatgcca ccgcacttgt ctcgtcctcg actatgaagc
     2701 cggtcacgcc gtcttcgacg atctccggca ccgaaccgcg gttataggcg atcactggcg
     2761 taccgcatgc catcgcctcg atcatcacga ggccgaacgg ctccggccag tcgatcggaa
     2821 cgagcaaacc gatcgcgccg gagagaaaat ccgatttctc gtggtcgttg atctcgccga
     2881 tgaagtcgac cagccgattg ctgttgatca gcggcgcgat cacgtggtcg taatagtcct
     2941 ggtcggcacg gtcgatcttc gcggcgattt tcagcggaat gccgcatcgc gtagcaatcc
     3001 tgatcgcgcg atcgacgccc ttttcgggcg cgatccgacc gagcacggca agatagccct
     3061 ggctcgacga tttcggcatc aacaaggact caggcaggcc atgatggatg gtccgcaccc
     3121 acctggcgtg cggtaccggg cggcgctgcg tgttcgaaat cgaaacgacg ggaatctcat
     3181 tgaacgtgtt gaagacgggc tggtgctcgg gcaggtcgag gcggccgtgc agggtggtga
     3241 ggaacggcgt cggctgccgg cagaacagcg agaaagggta ataatcgagg tggaagtgca
     3301 ggaagtcgaa ctcatcgtcg tcacattttc gccgcacccg ctccagcatc accatatgaa
     3361 gcgcgttcgg atcgcggacc gagccgtcga gacgcagcgc cttgggccag gttgcctcta
     3421 acttggccga ggtatgggaa tcgccgctgg caaacagagt gacctcgtgg cccagcgcca
     3481 ccagctcttc ggtcaaccaa tgcaccacgc gttcggtgcc gccataaagt ttcggtggaa
     3541 cagcctccgt cagcggagca acctgcgcaa tgcgcatctc acgacctcca agtttacaat
     3601 catgggtgaa gagccccccc ggtcgacagg cagccgactg ccgaacaggt gaacgttccc
     3661 gcatctgcga agttcctcac cccaacggtc acttctttga caccggcccg cgttaccacg
     3721 gttgcctcgg cggctaaccg ggtcgcggcc ccgatgttgc agcattgttg ggattgttga
     3781 acacaaatcg ctgcatcccg ggaaccgccc ggaagcgtca tacgttcaat cagggggaac
     3841 cggtacgccg tgtttgctct gacttagggg tcgtaaatca aatggatcag ttatcgggat
     3901 atgcgcatcg gccgcttcac tttgtcctgc gttacctgcg ccggcgacct gtttcgcatc
     3961 tcgtcatctt gactgccgtt gttgctgcgg ttgcctgttc ggtaggcacg caatacggcg
     4021 tgaaatatct ggtcgacagc ctggcggcgg ggccgggcct tgcgactcac gtatggctgg
     4081 cattcggtct gctcatgtcg ctgatcgctg ccgataattt tctttggcgc atcgcgagtt
     4141 ggacagcgag ttttaccttc gtgggagtaa ccggcgacct ccggcgcgac atgtttcgcc
     4201 acctgaccgg ccacgcgccg agctattttt ccgaccggct gccgggcatg ttgaccagcc
     4261 gcatcaccgc gacatcgaac gcggtgttca cggtcgaaaa catgttcgtc tggaatgtgt
     4321 tgccgccatg cattgcgacc gtcgccgcga tcgcgctgat cggaacggtc agcctttcta
     4381 tgtcggcggc gctgatcgtg cttgcgggga ccatggtggt tgccatgttt cgtctggccg
     4441 ccgccggcaa gccgctgcat gacgatttcg ccgacaaggc ggccatcgtc gacggcgaga
     4501 tggtcgacgt gatcagcaac atgccgctgg ttcgcgcatt ctgcggcttg agccacgagc
     4561 atgcccggtt cgacgcgacc gtcgggcgcg agctgatcgc gcgcggccgc agcctgcgct
     4621 atctcgaaaa gctgcgcatc ctgcacgccg gggtgacgct ggtgttgacc atcgcgctgc
     4681 tcgcctgggc gatccggctc tggcagcaag gcggcgccac caccggcgat gtcgttctgg
     4741 tgtgcacgct cggcctttcg attctcaacg ccacgcgcga tctcgccgtt gcgctcgtcg
     4801 acgtcaccca gcacgtcgcc cgcctgacgg aagcgatcgc gacactgctt ctgccgcacg
     4861 aactgcgcga tcacccggag gccgagccgc tgatgcggag cggagccgcg atcgccttcg
     4921 acaacatttc gttccagtat ccgggcggcg cgaaagtgtt cgatcggttc aacctgcgcc
     4981 tgcagcccgg ccaacgcgtc ggtttggtcg gtcactccgg cggcggcaag tccacgctgt
     5041 tcgtgctgct gcagcgtttc tatgacgtcg accaggggca catcatcatc gatggccagg
     5101 acatctcgcg ggttacgcag cagagcctgc gcgaggcgat ttcggtggtg ccgcaggaca
     5161 tttcgctgtt tcatcgctcg atcctggaga acatccgcta cgggcgcccg aacgcgaccg
     5221 acgacgaggt cttgcgcgcg gcgatcgcgg cccgctgcga cttcgtcgag acgctgcctg
     5281 ccggcctcga caccatggtg ggtgaccgcg gcgtcaagct gtcgggcggc cagcggcagc
     5341 gcattgcgat tgcgcgcgcc ttcctcaagg attccccgat ccttttgctc gacgaggcga
     5401 ccgcggcgct cgatgccgaa tccgaggagg ccatccgcga ggcgctgggc cgcctgatgc
     5461 gcggccgcac cgtgatcgct atcgcccatc gtcttgccac cttgcgcagc ttcgatcggc
     5521 tggtcgtgct gagatcgggc aagattctcg aggacggtcc tccggatcgc ctgatgcagg
     5581 tgcaggggcc atatcgcgag ctggtgacgc aggaaatgag ccggctggcg aaattcgccg
     5641 cgtgaagtga gcagtcgcgt tcggtctggt ttgcatcccg caggcccctg agaattgcgc
     5701 gaggttgcca tgacaagcga agcggtgagc caggtcctcg cgacgtcacg aacctacgaa
     5761 gtcgtcgtcg agcagccctt ctacatcccc atgaccgggc cggccgcgcg gctgcggcgg
     5821 tcgctcaagc atgacgacac cttcatcgtg ctcgacagtc atggcgacat cggcgcgtcc
     5881 gccggcggcc cggacgggct gttccatgcc gatacgcgct atctggcgcg attgcagctc
     5941 ctgttcgacg acctgcagcc actgcttttg ggctccaatc tgcgcga
//
LOCUS       DATLJT010001111         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1686782,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001111 DATLJT010000000
VERSION     DATLJT010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686782"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VM910_25660"
     CDS             <1..299
                     /locus_tag="VM910_25660"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVI65943.1"
                     /translation="ENNFAVYAIDQATGEPSLIRHVDTHGIHCRTFHIDPSGRLLVAA
                     HIMPLLVRDGTALRTISASLALFRIDDQGRLDFAGKYDVDVGDRTMFWMGMVAR"
     gene            complement(333..740)
                     /locus_tag="VM910_25665"
     CDS             complement(333..740)
                     /locus_tag="VM910_25665"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI65944.1"
                     /translation="MPTAVEDKDAIREVLAEYCFRLDEGRFDGMAELFTEDGTWDTAF
                     GKATGRAAIAELARRLRERGAQPRPRAVHLTTNIAIDVDGAHARVRSDWMVVQNSPQG
                     PKVGSGGAYIDEMVSADGRWLFRYRKIDRFIAP"
     gene            870..1811
                     /locus_tag="VM910_25670"
     CDS             870..1811
                     /locus_tag="VM910_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1410..1412,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HVI65945.1"
                     /translation="MRQREHGFGFTPEHDAPIPYMQRIREYYLALGYAAPYRWAHFAE
                     VPFQPLTKPLAECRVALITTAAPYQPDKGDQGPGAPYNAAAKFYSVYSGDSGTEHDLR
                     ISHLAIDRAHTTAEDSNTWFPLPQLRRSAEAGRISGVSPRFHGIPTNRSHRTTLETDC
                     PELLARCRADGADIAVLVANUPVCHQTVSLAARHLEENGIATVVMGCAKDIVEFVGVP
                     RFLFSDFPLGNAAGRPKDPQSQAFTLELALRILESAPTARTTVESPLRWSTDSSWKLD
                     YSNIERLSSEEIRQLREKFDRQKEIARDIRDKSAAPS"
     gene            2044..2778
                     /locus_tag="VM910_25675"
     CDS             2044..2778
                     /locus_tag="VM910_25675"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HVI65946.1"
                     /translation="MTKGNRSASSTPPVLLIRALTLVTVVSGLASGLFAGTAAAETPA
                     QFIYRVSHSTFGDIGSYSNTVEPTGAGTTVQTRAHFEVTMLGVRMHREDATRTERWQG
                     NRLVSFHGVTDKGDGPVEVKGEARGNDFVITSSQGTITAPASVHPANPWSNNFLSSNT
                     MMRPDSGKIEQVRIGSGQETTVKIDDATVSAVKFEIDGSTKYTVWLDGRGVPVKFVAD
                     DDTGKVTFTLAKCIGCGPELTQIGKR"
     gene            complement(2817..>3520)
                     /locus_tag="VM910_25680"
     CDS             complement(2817..>3520)
                     /locus_tag="VM910_25680"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HVI65947.1"
                     /translation="TTEQRHVTLRNGRLEADDFVLERNGFRFVHHDTKVVDFFDEDEV
                     RRVYYPEMQALVKAETGAARAEVFDHTLRTDDDELRETHKIREVVRRVHNDYTEWSGP
                     QRVRTFLPQEADELLHRRFAVIQVWRPIRHPVETWPLAIADAQSISPESLIVTERRYP
                     DRVGQTYAITYDPAHRWYWFPRMRPDEALVFKTYESQKQGCARWTAHTAFDDPTTVPG
                     ARPRESIEIRTLAFF"
BASE COUNT          661 a         1137 c         1079 g          643 t
ORIGIN      
        1 gagaaaacaa tttcgcggtg tatgcgatcg accaggcgac gggtgagccg agcttgatcc
       61 ggcacgtcga tacgcacggc attcactgcc ggacctttca catcgacccg agcggccgcc
      121 tactggtcgc tgcgcacatc atgccgctcc tcgtcaggga cggtaccgcc ttacgcacca
      181 tatcggcgag cctcgccctt ttccggatcg atgaccaagg caggctcgac tttgccggca
      241 aatacgacgt cgatgtcggc gaccggacga tgttctggat gggcatggtc gcgcgttagg
      301 cagctcgcct gcccacatca ttcctcagcg agtcacgggg cgatgaagcg gtcgatcttg
      361 cggtagcgga atagccaccg cccgtctgcg ctgaccatct cgtctatata ggcgccaccc
      421 gaaccgactt ttggaccctg cgggctgttc tgcaccacca tccaatcgga gcgcacccgg
      481 gcgtgcgcgc cgtcgacgtc aatcgcgatg ttcgtcgtca gatgcaccgc gcgcggtcgc
      541 ggttgcgcgc cgcgctcccg cagcctgcgc gccaattcgg cgatcgccgc ccgtccggtc
      601 gccttgccga atgccgtgtc ccaggtgccg tcctcggtga acagctccgc catcccgtca
      661 aaccgaccct cgtccaggcg gaagcagtat tccgccagca cttcccgaat tgcatccttg
      721 tcttctaccg ctgtgggcat cgctatttcc tccggcattt ggcgttgcgg cgcacttgaa
      781 ccggttcgat actgcaagcc gcgagccgcg ccgcacaagc cacttgtcgg cttccgccag
      841 cgacggcccg ttcacgagag agggaccaga tgaggcaaag ggagcacggc ttcgggttca
      901 ctcccgaaca cgatgcgccg atcccctata tgcagcggat ccgcgaatat tacctggcac
      961 tcggctatgc agcgccttat cgctgggcgc attttgccga ggtgccgttc cagccgctca
     1021 caaagccgct cgctgagtgc cgtgtcgcct taatcaccac cgcggccccc taccagcccg
     1081 acaaaggcga tcagggcccc ggcgcccctt acaacgccgc cgccaaattc tatagcgtgt
     1141 attccgggga cagcggcacc gagcacgatc tgcgaatttc ccatctcgcg atcgaccgcg
     1201 cccataccac ggccgaggac agcaacacct ggtttccgct gccgcaattg cgacgctctg
     1261 ctgaggcggg acggatcagc ggggtctcgc cacgctttca cggtataccg acaaaccgca
     1321 gccaccgcac gacactcgaa acagactgcc ccgagctcct ggcgcgctgc cgcgccgatg
     1381 gtgccgacat cgcggttctc gtcgccaatt gaccggtgtg ccaccagacc gtgagtctgg
     1441 ccgcccggca tttggaggag aacggcattg ccaccgtggt gatgggttgc gccaaggaca
     1501 tcgtggaatt tgtcggcgtg ccgcgcttcc tgttcagcga tttcccgctc ggcaacgcgg
     1561 cgggacgtcc aaaggacccg caatctcaag ccttcacact ggaactcgcc ctgcgtatcc
     1621 ttgaatcggc gccaacggca cgcacgaccg tggagtcgcc gctgcgttgg agcacggatt
     1681 cgtcatggaa gctcgactac tccaacatcg agcgcttgtc ttctgaagag atccggcagc
     1741 tgcgagagaa attcgaccgg cagaaagaga tcgccaggga tatccgcgac aaatccgcag
     1801 caccgtcgtg aaaatgaacg gagcggggcc agaaaatttc tttggatggg cacagaccga
     1861 agcctaggca ggtcgccaac atctgcgctt aacggatcac aacgacggcc gtgccggccg
     1921 catgcgacgc gatattcttt ttgtatgaag ggctgtcgct tgtgtgaagg gctgtcgcgc
     1981 ggtatcctgt ggggacggtc tccggtttgg tacggagagc ccgccacttg gtgaggggga
     2041 aacatgacca agggcaatcg aagcgcgtcc tcgacgccgc cggtgttgct cattcgggcc
     2101 ctgacgctcg tgacagtcgt cagtggcctt gcctctggcc ttttcgccgg caccgccgcg
     2161 gcggagactc cggctcaatt catctatcgg gtttcgcact ccacatttgg cgatatcggc
     2221 agttacagca acacggtcga gcctaccggg gcgggtacaa ccgtccagac gcgggcgcat
     2281 ttcgaggtga ctatgctggg cgtgcggatg catcgcgagg atgccacgcg caccgagcgc
     2341 tggcagggca accgcctcgt ttcatttcat ggcgtgaccg acaagggcga cggcccggtc
     2401 gaggtcaaag gcgaggcgcg cggcaacgat ttcgtgatca cctcgtcgca aggcacgatc
     2461 accgcacccg cgtcggtcca tccggccaac ccgtggtcaa acaacttcct cagctccaac
     2521 acgatgatgc gccccgattc cggcaagatc gaacaggtgc gaattggcag tgggcaggag
     2581 acgacggtga aaatcgacga tgccacggtc tcggctgtta aattcgagat cgacggcagc
     2641 accaaataca ccgtttggct cgacggacgg ggcgtgccgg taaagttcgt ggccgacgac
     2701 gacaccggca aggtgacctt caccctggcc aaatgcatcg gttgcggccc ggaactcacc
     2761 cagatcggga agaggtaatc ccgaccatcg ggtaggaagg gtaggaggcc ggcagctcag
     2821 aaaaaggcga gcgtgcggat ctcgatgctt tcgcgcggcc gcgccccggg cacagtcgtc
     2881 gggtcgtcga aggccgtatg cgcggtccac cgcgcgcagc cttgcttttg cgactcgtag
     2941 gttttgaaga ccagcgcctc gtccggccgc atccgcggga accaatacca gcgatgcgca
     3001 gggtcgtagg tgatcgcgta ggtctggccg acacggtcgg gatagcggcg ctcggtcact
     3061 atcaggctct cgggcgagat gctctgcgca tccgcgatcg ccagcggcca ggtttcgacc
     3121 ggatgacgga tcggtcgcca cacctgaatc acggcaaagc ggcgatgcag gagctcgtcg
     3181 gcttcttgcg gcagaaaggt gcgcacccgc tgcgggcctg accactcggt gtagtcgttg
     3241 tggacgcgtc gcacaacttc acggattttg tgggtctcgc gcagctcgtc atcgtcggtg
     3301 cgcagggtgt gatcaaacac ctcggcgcgc gcagcgccgg tctcggcctt taccagtgct
     3361 tgcatctccg ggtaatagac acggcgcacc tcgtcctcgt cgaagaaatc gacgaccttg
     3421 gtgtcatggt ggacaaagcg aaacccgttg cgctccagca caaagtcgtc ggcctcgagc
     3481 cggccgttgc gcaacgtgac atggcgctgc tcggtcgtgc
//
LOCUS       DATLJT010001112         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_995930,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001112 DATLJT010000000
VERSION     DATLJT010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995930"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VM910_25685"
     CDS             complement(<1..1251)
                     /locus_tag="VM910_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HVI65948.1"
                     /translation="MTRGVRLYLVGSFVLVSLAGCGRGFFQAEREPWRAEAEIACLKS
                     GAVKESLDVVRVEPISGPGACGADFPLRVAAFGEGSSSFGFADEDLRPPAPIGNQPRW
                     PISQPPPPTYPQNSYPQPSYPQSSYPRTSYPRASNYPQAYPETVVREPRYGAAPGAPI
                     SLSAPGVRVDEDAINLPPEGTPATRAPYAPPYPPRDRYGAPYSPRPDANESLPRLGPR
                     GNPIGAVGPVAVKPAATLACPIVSVLDHWLADSVQPAAQRWFGARVVEIKQISAYSCR
                     GMNGNPHAHISEHAFGNALDIAAFTLADGRRITVKDGWRGLPEEQGFLRDVQAAACQR
                     FSTVLAPGSNVYHYDHIHVDLMRRASGNIICEPDAVSGEEIASRAAHRNPYASRGPFV
                     TGSLGGQRQAPRKRAREKVDEEDEF"
     gene            complement(1587..2525)
                     /locus_tag="VM910_25690"
     CDS             complement(1587..2525)
                     /locus_tag="VM910_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI65949.1"
                     /translation="MRRLSYLAMLMVLCSYGSSAQAGYSFLIGGHHIYIDARGGCRSL
                     SCVSWSGTHGWRHRRDREEDVVKISNTKPATTAPAPTPAAAPPQPAPVVQAPPPPAPA
                     PVAQVQPAPAAPPAVTAPATKLAAETVAPPQPAPATNTDLATTTQSVPSPATKSEPAK
                     TVVIEKPVETPVAPPPPSTQLAAEPARAKQDFADPDSPLGDWQTEGNKGLVRIEPCGR
                     ALCGYVLNAATNAKGETVLSNMKAKTDSQWTGDIYSRASGNSYFAKMTLKAPNTLHVE
                     ACAFSRFFCSGNDWTRVVTKPDEVIISRQSTPEPKS"
     gene            2696..>3168
                     /locus_tag="VM910_25695"
     CDS             2696..>3168
                     /locus_tag="VM910_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI65950.1"
                     /translation="MKSLYLTAAAALLLTSTTAFAGNNSVSFQIEGQKIHVEQPKGCN
                     SLSCIQITAPGLSKSFNLKNLNLDGFKSKDDDDDVATTTTPTQPAPQPAPAPAAAPAP
                     QAPAPVVNTVPPAPPAAAPAPAPVTSYAPPAIAPAPAPQTPQVATVAPAPQAAPVA"
BASE COUNT          534 a         1017 c         1037 g          580 t
ORIGIN      
        1 gaactcgtct tcctcgtcga ccttctcgcg cgccctcttg cggggcgcct gccgctggcc
       61 gccgagcgat cccgtgacga acggtccacg cgaagcataa ggattgcggt gcgcggcgcg
      121 cgaggcgatc tcctcgcccg acactgcgtc cggttcgcag atgatgttgc cgctcgcccg
      181 ccgcatcagg tcgacgtgga tgtgatcgta gtgatagacg ttcgagcccg gcgcgagcac
      241 ggtcgagaac cgctggcagg cggccgcctg cacgtcgcgc aaaaatccct gctcttccgg
      301 caggccgcgc cagccgtcct tcaccgtgat gcggcggccg tctgcgagcg tgaaggctgc
      361 gatgtcgagc gcgttgccga aggcgtgctc ggaaatatgc gcgtgcggat tgccgttcat
      421 gccgcggcag gaataggcgg agatctgctt gatctcgacc acgcgggcgc cgaaccagcg
      481 ttgcgcagcc ggctgcacgg aatcggcgag ccagtggtcg agcacggaga cgatcggaca
      541 ggcaagcgtt gccgccggct tcaccgccac cggcccgacc gcgccgattg gattgccgcg
      601 cggcccaagc cgcggcaggc tctcatttgc atcgggccgc ggcgaatagg gcgcgccata
      661 gcgatcacgc ggcggatagg gtggcgcata gggtgcacgc gttgccggcg tcccctccgg
      721 cggcaggttg atcgcgtcct cgtctactcg cacgccaggc gccgacagcg agatcggcgc
      781 gcccggagca gcgccatagc gcggttcgcg cacgactgtt tccgggtagg cctgcggata
      841 gtttgacgct cgcggataag acgttcgcgg ataagacgac tgcggataag acggctgcgg
      901 ataggaattc tgcggatagg ttggcggcgg cggttgcgag attggccagc gtggctggtt
      961 gccgatcggc gccggcggcc gcaaatcttc atcggcaaag ccgaaactgg acgaaccctc
     1021 gccaaacgct gcgacccgca acggaaaatc cgccccgcag gcgcccggac cagagatcgg
     1081 ctcgacgcgg acgacatcga ggctttcctt caccgcgccg gacttcaggc aggcgatctc
     1141 ggcctcggcc cgccacggct cgcgctcggc ctggaaaaat ccgcggccgc agcctgccag
     1201 cgaaacaagg acgaaggagc cgacgagata caaacgaact ccacgcgtca tgcgcgcacg
     1261 ttcgatgaat ttatttaagg actcatcaac gcgttgattt cacgtatctt taaccacgaa
     1321 tgccagcttg ctgcagcgca ttgacagtgc ggcttacttt ttcacccatc gtcgctctgc
     1381 taatttgctg tgcagatgcg ggcagtgacg taagagaggg actattccca tgaccttcgt
     1441 caacaggctg agcgtcatca ccgcgtatgt cgcatttgct ttcgtcggcg cgattgtgct
     1501 cggcgtcttc tagaagttcg ggagagctca aggaagcatc accggtttga acgcccggcg
     1561 gtttcgccgg gcgttttcgt tttggctcaa gatttcggtt caggcgtgct ctgtcgcgag
     1621 atgatcacct cgtcgggctt ggtcacgacc cgcgtccagt cgttgccgga gcagaagaag
     1681 cggctgaagg cgcaggcttc cacatgcagc gtgttcggcg ccttgagcgt cattttggcg
     1741 aaatagctgt tgccgctggc acggctgtag atgtcgccgg tccattggct gtcggtcttc
     1801 gccttcatgt tggagagcac ggtctcgccc ttggcgttgg tcgcggcgtt gagcacatag
     1861 ccgcacagcg cacggccgca gggctcgatg cggaccaggc ccttgttgcc ttcggtctgc
     1921 caatcgccga gcggcgaatc ggggtcggcg aaatcttgct tcgccctggc gggctcggct
     1981 gcgagctgcg tcgacggcgg tggtggggcg accggcgttt cgaccggctt ttcgatcacc
     2041 acggtcttgg cgggctcgga tttggtcgca ggcgacggca cggattgcgt ggtggtggcg
     2101 agatccgtat tcgtcgcggg tgcgggttgc ggcggcgcga cggtctcggc ggcgagcttg
     2161 gtcgcgggtg cggtcactgc gggaggagct gccggcgcgg gctgaacttg cgcaaccgga
     2221 gctggggcgg gaggcggcgg tgcttgcacc accggagccg gctgaggtgg cgctgcggcc
     2281 ggagttggcg caggcgccgt tgtggccggc ttggtgttgg agatctttac cacatcctct
     2341 tcgcgatcgc ggcgatgacg ccagccgtgc gttcccgacc aggaaacgca ggacagcgac
     2401 ctgcagccac ctcgcgcatc gatgtagata tgatgaccgc cgatcaggaa ggaatagccg
     2461 gcctgcgcgg acgaaccgta ggaacagagc accatcagca ttgccagata gcttagtcgc
     2521 ctcatggggg cctcccctgc tgtccggggc aagctcaaca ccgtttgttt ttcctgactg
     2581 tgatgtgaat cacgcgcctg tcgccggcct tgtcccgtga cgccgatcac agaagcttgc
     2641 aaacggccgg cctagtctgg cgacgaaatc aagcgatccg tcgagggaga caatgatgaa
     2701 gagcctttat ctcactgccg ccgcagcgct gctgctgacc agcaccacgg cgtttgccgg
     2761 caacaactcg gtctcgttcc agatcgaggg acagaagatt catgtcgagc agcccaaggg
     2821 ctgcaactcg ctgtcgtgca tccagatcac ggcgcccggt ctctccaaga gcttcaatct
     2881 gaagaatctc aatcttgacg gcttcaagtc gaaggatgat gacgacgacg tcgcgaccac
     2941 cacgacgccg actcagcccg cgccgcagcc cgcgcccgcc cctgccgccg cgcctgcacc
     3001 gcaggctcct gctccggtcg taaacaccgt tccgccggcg ccgcccgctg ctgctcctgc
     3061 gcccgcaccg gtcaccagct acgccccacc ggccattgct cccgctcccg ccccgcaaac
     3121 cccgcaggtc gcaaccgttg cgcctgcgcc gcaagctgct cccgttgc
//
LOCUS       DATLJT010001113         1622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_995934,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001113 DATLJT010000000
VERSION     DATLJT010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995934"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VM910_25700"
     CDS             complement(<1..1452)
                     /locus_tag="VM910_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI65951.1"
                     /translation="MTDAVVARIERVTHRYGRRFALNDLTLDIPARCMVGMIGPDGVG
                     KSTLLGLISGVRKIQTGKVTVLDGNMADGAHRRASYGRIAYMPQGLGRNLYPTLSVFD
                     NIDFFGRLFGQGTAERRARIDVLLPATGLDPFASRPCGKLSGGMKQKVSLCCALMHDP
                     DLLVLDEPTTGVDPLSRRQFWELIDSIRARRPAMSVIVATAYMDEASRFDWLAAMDDG
                     KVIARGTPKEILALANKTSLDDAFIALLAPEKRAQHQEVIVRPRTISADETPAIEADG
                     LTRRFGDFVAVDHVSFRIARGEIFGFLGSNGSGKTTTMKMLTGLLPVTEGSAKLFGKP
                     MGADDMEARHNVGYMTQAFSLYSELTVRQNLDLHARLYHLPANEIEGRIKELLERYDL
                     KDVADARPESLPFGVKQRLQLAVAVLHRPALLILDEPTSGVDPVARDAFWRSLIDLSR
                     NDGVTIFLSTHFMNEAERCDRISLMHRGRVLAVG"
     gene            complement(1449..>1622)
                     /locus_tag="VM910_25705"
     CDS             complement(1449..>1622)
                     /locus_tag="VM910_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HVI65952.1"
                     /translation="ETAEERQKLMFRVKLQGDPKVLDKYHRQVKTGVRGLGFVRTDPK
                     IAWPEELVVKLPQ"
BASE COUNT          292 a          503 c          525 g          302 t
ORIGIN      
        1 gccgaccgcg agcacccgcc ctcggtgcat cagcgaaata cggtcgcagc gctcggcctc
       61 gttcatgaag tgagtcgaaa ggaagatcgt gacgccgtca ttgcgggaga gatcgatcag
      121 cgagcgccag aacgcgtcgc gtgcaaccgg atcgacgccc gaagtcggct cgtcgagaat
      181 gagaagggcg ggccggtgca gcaccgccac ggcgagctgc agccgttgct tgacgccgaa
      241 cggcaagctt tccggcctcg cgtcggccac gtccttgaga tcatagcgct ccagcagctc
      301 cttgatccgg ccttcgatct catttgccgg cagatggtag agacgcgcat gcagatcgag
      361 attctgccgg acggtgagct cgctgtagag ggaaaatgcc tgcgtcatgt agccgacgtt
      421 gtgccgcgcc tccatgtcgt cggcgcccat cggctttcca aaaagtttgg ccgagccttc
      481 ggtcaccggc aggagccccg tcagcatctt catggtggtt gtcttgccgg agccgttcga
      541 gccgaggaag ccaaagatct cgccgcgcgc gatgcgaaaa ctcacgtggt cgacggcgac
      601 gaaatcgcca aagcggcggg tcaggccatc cgcttcgatc gccggggtct cgtcggcgct
      661 gatggtgcgc gggcgcacga tgacttcttg atgctgggcg cgtttttccg gcgccagaag
      721 ggcgatgaag gcgtcgtcga gcgaggtctt gtttgctagg gcgaggatct ccttcggcgt
      781 gccacgcgcg atgaccttcc catcatccat ggcggcaagc cagtcgaagc ggctggcctc
      841 gtccatataa gccgtcgcga cgatcacgct catggccgga cgccgggcgc ggatcgaatc
      901 gatcagctcc cagaactgcc ggcgcgagag cggatcgacg ccggtcgtcg gctcgtcgag
      961 aacgagcagg tccggatcgt gcatcagcgc gcagcagagg ctgacttttt gcttcatgcc
     1021 ccctgagagc ttgccgcacg gccggctagc aaacggatcg agacctgttg ccggcaagag
     1081 aacgtcgatg cgcgctctgc gctcggctgt gccctgcccg aacaagcggc cgaagaagtc
     1141 gatgttgtcg aacacgctga gcgtgggata gagattgcgg cccagcccct gcggcatata
     1201 agcgatacgg ccgtaactcg ctctgcgatg cgcgccgtcc gccatgtttc cgtccagcac
     1261 cgtgaccttg ccggtttgga tcttgcgcac tccggagatg aggccgagca aggtggactt
     1321 gccgacgccg tcgggcccga tcattcccac catgcagcgg gcaggaatat cgagggtgag
     1381 gtcgttgagt gcgaacctcc tcccgtagcg gtgcgttacc cgttcgatcc tggcgacaac
     1441 ggcgtcggtc attggggaag cttcaccaca agttcttcag gccaggcgat cttggggtcg
     1501 gtgcgcacga agccgaggcc gcgcacgccc gtcttcacct gccgatggta tttatccaaa
     1561 accttgggat ctccctggag cttgacgcgg aacatcagtt tctgccgctc ctcggcggtc
     1621 tc
//
LOCUS       DATLJT010001114         1923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2835956,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001114 DATLJT010000000
VERSION     DATLJT010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835956"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1923
                     /locus_tag="VM910_25710"
     CDS             <1..>1923
                     /locus_tag="VM910_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65953.1"
                     /translation="HWYDTLTVGLLNELVVQARDARLLLVVSYRPEYDDQWRNRPNYR
                     QLRVYPLISDNLGEFLDVLLGSDPSLEGLKSFLAERTSGNPFFVEEIVRTLVDTGVIE
                     GERGNFHLTTRIWNMLVPPSVQAVLAARIDRLPAAEKQLLQEAAVIGQDVPFNLLQVI
                     CGLAEEPLRHLLEKLQSAEFIYITQLFPDLQYGFRHSLICDVAYGGVLREHRRAAHAR
                     VIEAMEGLYSDRLIEQVERLAYHSVRGELREKAAHYLRQAGAKAAARGALQEARTSFE
                     QALNILKSLPDSSEAMEEAFEIRLALRTVLRQLGEVELMLEHLREAEVLAERLNDDVR
                     RCRVFSFLTLVLSNLGQVNEAAVVGARSVEIARRIGDLRLSIITGSNLAEPYYLRGEY
                     EQAVETAAGTLAALPSQLALEYFGIAVPPSVFARGWLVMSLAALGRFDEAAGRVAEMI
                     ELAAKTKHVHTIGWAHLTASRLHLVKGDWTKVRDLLEHWMSMPGTLDVTTLLPWAVTS
                     LAWALAQIGEADQALSRVQEGEEHLKRQDAKGIFIHRDWGYHAVARACLLLGRLDQAR
                     RLADHSLESSQRQPGFAAHAQCMLGDLAIQTDRFDAECATAHYAKALALGESRGMRPV
                     VAHCHLGLGRLHRRSGN"
BASE COUNT          367 a          564 c          594 g          398 t
ORIGIN      
        1 cactggtacg atacacttac tgttggactg ctcaacgagc tggtggttca agcgcgcgac
       61 gcccgtttgc tgctcgtcgt cagttatcgt ccggagtatg acgatcaatg gagaaatcgc
      121 ccgaactatc gccagctacg cgtctatccc ctcataagcg ataacctcgg ggaatttctc
      181 gacgtattgc tggggtccga ccccagtctg gaaggcctga agagttttct cgcggagcgc
      241 accagcggca atccattctt cgtcgaagag atcgtgcgaa cgctggtcga taccggagtg
      301 atcgagggcg agcgtggcaa tttccatctc acaacgcgga tttggaacat gctcgtgccg
      361 ccgtcggtgc aagccgtgct ggccgcgcgt attgacaggt tgcccgccgc cgagaaacag
      421 ctgctgcagg aggccgcggt catcggccaa gacgtcccgt tcaacctgct gcaggtaatt
      481 tgcgggttgg cggaggaacc gctccgacat ctcctcgaga agctgcagtc cgcagaattc
      541 atctacatca cacagctatt ccccgatttg caatacggct tcaggcactc cctcatttgc
      601 gacgtcgcct acggcggggt actccgcgag catcggcgcg ctgcacacgc tcgtgttata
      661 gaggcgatgg aaggacttta ttctgatcgt ttgattgagc aggttgagcg cctcgcctat
      721 cattccgtgc gcggcgaact tagggaaaag gcagcgcact acttgcggca ggccggagcg
      781 aaggcggcgg cgcgaggggc tctccaagaa gcgcgaactt ctttcgaaca ggcgctcaac
      841 attctcaaat ccctgccgga cagctcggag gcgatggaag aagccttcga gattcgtctc
      901 gcattgcgga cggtgctacg gcagcttggt gaggtcgagc tgatgctcga gcatcttcgc
      961 gaggcagagg tgcttgccga acggttgaat gacgatgtgc gacgttgccg cgtttttagt
     1021 ttcctgacgc ttgtcctgtc aaacctgggt caggtgaacg aggcagccgt ggtcggcgct
     1081 cgctccgtag aaattgcccg gcgtatcggg gacctgcggc ttagcatcat cacgggaagc
     1141 aacctcgcgg agccgtatta cctgcggggc gagtacgagc aagctgtcga gaccgctgct
     1201 ggaaccctcg ccgcactgcc cagccaattg gccctcgaat atttcggcat agccgtgccg
     1261 ccatcggtct ttgcccgagg ctggctggtc atgagcctag cggcgcttgg caggttcgat
     1321 gaggcggccg gacgtgtcgc ggaaatgatt gaactcgcgg cgaaaacgaa gcatgtgcac
     1381 actatcggct gggcccactt gaccgcaagc aggcttcacc tcgtgaaggg cgactggacc
     1441 aaggtgcgcg acttactcga gcactggatg agtatgccgg ggactctaga tgtgactacc
     1501 ctccttccct gggcagtcac ctccttagcc tgggcgctgg cgcagattgg cgaggccgat
     1561 caggcgttga gtcgcgtgca agagggtgaa gaacacctta agcgtcagga cgccaaggga
     1621 atctttatcc atcgggactg gggctatcac gcggtagctc gcgcctgcct cctgctcggt
     1681 cgactcgacc aggcccggcg tttggcagat cattcgcttg aatcatcgca gcgccagccg
     1741 gggttcgccg cccatgcaca atgcatgctc ggcgatctag ccatccagac cgatcgcttc
     1801 gacgccgaat gcgccaccgc tcattacgcg aaagctctgg cgctcggcga gtcgcgcggg
     1861 atgcggccgg tcgtcgctca ttgtcatttg ggcttgggtc ggcttcatcg ccgctctggc
     1921 aat
//
LOCUS       DATLJT010001115         7890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2145492,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001115 DATLJT010000000
VERSION     DATLJT010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145492"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VM910_25715"
     CDS             complement(<1..761)
                     /locus_tag="VM910_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI65954.1"
                     /translation="MTTNKKKIFVTQTMSPRARALLTERDDIELVEFPNLISAKDFEA
                     MLKQHAPVHGVALGATRFGEPELEACRDMKVVTRIGVGYDAVDVPALSRRKVPLMVAG
                     TANSPSVAEQALFMMLMLAKRATEMHEMVMNGTWASRLSQLPFDLYGKTLLIVGFGRI
                     GTRTAKRCLAMEMRVLVYDPYKQAAEIVSAGCETVADLDAALPRADFVSIHCPKTPET
                     VGMFNAARLKLMKPTAYLINTARGGIVDEKALHEAL"
     gene            complement(808..1908)
                     /gene="ugpC"
                     /locus_tag="VM910_25720"
     CDS             complement(808..1908)
                     /gene="ugpC"
                     /locus_tag="VM910_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HVI65955.1"
                     /translation="MADVSLRKVVKRYDDVEAVRGIDLDIADHEFVVLVGPSGCGKST
                     TLRMIAGLEDITDGDIVIGGDVVNDVPPKDRDIAMVFQNYALYPHMTVAENMSFALRL
                     KHFPKGEIKSRVDEAARMLDISDLIDRKPKQLSGGQRQRVAMGRAIVRHPKVFLFDEP
                     LSNLDAKLRVQMRIEIKKVHQKVRTTTVYVTHDQVEAMTLADRVVVMNKGRIEQIGTP
                     NELYHKPATRFVAGFIGSPAMNFLPCRLEDAGGRLNIRLTDRLSFPLPPQRAARYQNV
                     PRNDKLLLGIRPEHLTETSAHLESGVEPFDAVLDVTEPMGMETLVYFTLEGNQVCGRV
                     SPGAGARDGAPLRLAVDLNNMHLLNEGTGAVL"
     gene            complement(1915..2826)
                     /locus_tag="VM910_25725"
     CDS             complement(1915..2826)
                     /locus_tag="VM910_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVI65956.1"
                     /translation="MSSLAIDKAGPRRKVKYGSMSRDRVWALRWSYFFLALFAIFSLV
                     PPIYMLITSLKSSAEISAATNPWWVYHPTLENYIALLTSNQFLRFFWNSAWVSIIVVA
                     VTMLISVPAAFALARMKFWGSATLATGVFLTYLIPDSLLFIPLFKMFALFGDWTGIPL
                     INHWYVLLLVYPTLTVPFCTWIMIGYFASIPKELDEAALIDGASWLQTLTRIFIPVAL
                     PGLIAATIFAFTVSWAQFLYPLVFTTSTDQLVMPVGIITSLIKGDVFNWGQIMTGALL
                     GAAPPLLIYAFLMDYYIAGLTAGATKG"
     gene            complement(2826..3758)
                     /locus_tag="VM910_25730"
     CDS             complement(2826..3758)
                     /locus_tag="VM910_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI65957.1"
                     /translation="MVDVVFQSTRVAESRRAGKSLKVALQRKSTIAFLMTLPLILLIA
                     LLVLYPAIYAVHLATLNKAMTKFVGLGNFEFLFKRETFWMVVEQSCIFAITAVIFKAL
                     IGFIVAHFAHNIPTKKQRKWRGMLLVPWVIPPAMSTLAWLWLFDPSYSAFNYTLGFFG
                     IGPISWLGEVGWARFSVILVNVWYGAPFFMIMYLAALKSVPEQLYEAAAIDGANWWQR
                     IWYVTLPMMRNIIAITTLFSLIVTFANFDITRILTAGGPLDHTHIFATWAFRVGIEGS
                     DIPLGASVSLFMAPILAIAAVFILRDISKRGNEA"
     gene            complement(3872..5215)
                     /locus_tag="VM910_25735"
     CDS             complement(3872..5215)
                     /locus_tag="VM910_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI65958.1"
                     /translation="MSRKKLTRRQFVAATAASTAALVTAPYVRSARAAGSLSIGFWDH
                     WVPGANKTSTDLVNEWAAKEKVEVQIDYITSQGNKLLLTQTTEAQAKSGHDILASGGP
                     WQPQAFAELLEPMNDIMEPILKENGDVNGTVKYLGQAGGKWLAVPACIGSQIKGPCSR
                     IDLMKKYANIDVQAMYPAGAPPKADNWTMDTFLKAAEDCQKGGYPFGIGLGETTDSVD
                     TAGAIFQSFGAELVTANGDLTVKTDAVRQALEYYKKLISFLPPDVAAWDDASNNKWLI
                     SGKGALIMNPPSAWAVAKRDAPQVAEQCWTHGFPAGPKGRFAPYLPFFWSVWNFSKNK
                     AAAKSLLTHLSQPDSIERLVNASGGYDLPAFEKLTTLKAWAEQEPPKGTLYHYPNPYK
                     HQKLSIAASPAPPKIAQQIYTQATLTKMCLRYARGEKMESTLAWVEGECEGFMRS"
     gene            5724..6362
                     /locus_tag="VM910_25740"
     CDS             5724..6362
                     /locus_tag="VM910_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI65959.1"
                     /translation="MVGAQGLVKGVEQGAREGTKAAGPVGGVLGGAIGGVVGVVTGVT
                     DALTGKSGQPVANDKANDKGKAAKTAKATTANNPKAGKPIQVLTQQGAPQLTAEQIVA
                     NSDAYIERIKAELNLTPEQEKSWSSFSSAMHYLGHNGADRLNLRIERAKRDPPDDIIE
                     QMRNEAQFLVDRAQDQRNVADAAEPLYASLDGQQKQKFIDEMVRLSHERGLD"
     gene            complement(6632..7033)
                     /locus_tag="VM910_25745"
     CDS             complement(6632..7033)
                     /locus_tag="VM910_25745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI65960.1"
                     /translation="MPLGGLQHFTIEPQDLERSKNFYVNVLGLEVGDRPPLDFPGYWL
                     YSGGNATVHLMGTRKPREGIVVRGTDKRFEDTGRLDHIAFAATDVDGVRKRLIANSVK
                     FREQIVPRTGDTQFFLYDPDGVGVELNFPKA"
     gene            complement(7069..>7890)
                     /locus_tag="VM910_25750"
     CDS             complement(7069..>7890)
                     /locus_tag="VM910_25750"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI65961.1"
                     /translation="PKVKAIECEGGYRVTGVWAFASGGRHATWLGAHCPIFKADGSPR
                     LEDNGVQAERTMLVRSEDVEWTDIWNTVGLRGTASDQFALNDFFVRADHSIIREFDRE
                     CRESGPLYRMGNGTCYQVGFAGVACGIARGALDCFVVLARDKVIRGGRTPIRENAVVQ
                     SGLAQAEVNLRAARGFVLQSMAEIWKHLCAGAMITVAQRITVRMAATNAIHKAREAVD
                     LAYHAAGATSIFDDHPLERRFRDIHTVTQQLQGRASHFQTVGAWMMGAEADLTWV"
BASE COUNT         1575 a         2377 c         2457 g         1481 t
ORIGIN      
        1 agcgcctcat gcagcgcctt ttcgtcgacg atcccgccac gcgccgtgtt gatcagatac
       61 gccgtcggct tcatcagctt gagccgcgcc gcgttgaaca tgccgacagt ttccggcgtc
      121 ttcggacaat ggatgctgac aaaatcggcg cgcggcagcg ccgcgtcgag gtccgcgact
      181 gtctcgcaac ccgctgatac aatttcggca gcctgcttgt agggatcata gaccagcaca
      241 cgcatctcca tggcaaggca tcgcttcgcc gtgcgcgtgc cgatgcggcc gaagcctacg
      301 atcagcagcg tcttgccata gaggtcgaac gggagctggc tcagccggct cgcccaggtg
      361 ccgttcatga ccatctcatg catttcagtc gcgcgcttcg ccagcatcag catcatgaac
      421 agcgcttgtt cggcgaccga tggcgagttc gccgtacccg cgaccatcag cggcaccttg
      481 cggcggctca gtgccggtac gtcgacagcg tcgtagccga cgccgatccg ggtcaccacc
      541 ttcatgtccc tgcaggcttc gagctcgggt tcgccgaagc gggtggcgcc gagcgcgacg
      601 ccgtgaaccg gcgcgtgctg cttcagcatc gcctcgaaat ccttggctga gatcaggttg
      661 ggaaactcga ccagttcgat gtcgtcccgc tcggtcaaaa gagcccgcgc cctgggcgac
      721 atggtttgcg tgacaaaaat ctttttcttg ttggtcgtca tttcctgccc ttggggtttg
      781 ccagtggtgc gataccacta gccggcgtca aagaaccgcg ccggtcccct cgtttagcag
      841 gtgcatattg ttgaggtcca cagccaatcg gagaggagca ccgtcgcgcg cgccggcgcc
      901 cggactgacc cggccgcaga cctggttgcc ctcaagcgtg aaataaacga gcgtctccat
      961 tcccatcggc tcggtgacgt cgagcaccgc gtcgaacggc tcgacgccgg attcgaggtg
     1021 tgcgctggtc tcggtgaggt gctcgggacg gatgccgagc agcagcttgt cgtttcgtgg
     1081 cacgttctgg tagcgcgccg cgcgctgggg cggcagcgga aacgagaggc gatcggtgag
     1141 gcggatgttg agcctgccgc cagcgtcttc gagccggcaa ggcaaaaagt tcatcgcggg
     1201 cgagccgatg aaacccgcga cgaagcgcgt cgccggtttg tgatagagct cgttcggcgt
     1261 cccgatctgc tcgatgcggc ccttgttcat gaccacgacg cggtcggcga gcgtcatcgc
     1321 ctcgacctgg tcgtgggtta cgtagaccgt cgtggtgcgg accttctggt gcactttttt
     1381 gatctcgatc cgcatctgta cgcgaagttt cgcgtccagg tttgagagtg gttcgtcaaa
     1441 cagaaacacc ttggggtgac gcactatggc gcgtcccatc gccacgcgct gccgctggcc
     1501 gccggacaac tgctttggct tgcggtcgat caaatcggag atgtcgagca tacgggcggc
     1561 ttcgtcgacc cggctcttga tctcaccctt cggaaaatgt ttcagccgca aggcgaacga
     1621 catgttctcg gcgaccgtca tgtgcgggta aagcgcgtag ttctgaaaca ccatggcgat
     1681 gtcgcgatct ttcggcggca cgtcgttgac gacgtcaccc ccgatcacga tgtcgccgtc
     1741 ggtgatgtct tcgagaccgg caatcatccg cagcgtggtg gacttgccgc agccggacgg
     1801 accgaccagc accacgaact cgtggtcggc aatatcgaga tcgatgccgc ggaccgcctc
     1861 gacgtcgtcg tagcgtttca ccaccttacg caagctgacg tcagccatcg attttcaacc
     1921 tttcgtcgcg ccggcggtca gaccggcaat gtagtagtcc atcaggaagg cgtagatcag
     1981 gagtggcggc gcagcgccaa gcagcgcgcc tgtcatgatc tgcccccagt tgaagacgtc
     2041 gcccttgatc aggctcgtga tgatgccgac gggcatgacg agctgatcgg tcgaggtggt
     2101 gaagaccagc ggatagagaa actgcgccca ggataccgtg aaggcgaaga tcgtcgcagc
     2161 gatcagacca ggcagcgcga ccgggatgaa gatgcgcgtc aatgtctgca gccaggacgc
     2221 accgtcgatg agggcagcct catcgagctc cttcgggatc gacgcgaaat acccgatcat
     2281 gatccaggtg cagaacggca cggtcagcgt cggatagacc agaagcagga cataccaatg
     2341 gttgatgagc gggattcccg tccaatcgcc gaacagcgcg aacatcttga acagcggaat
     2401 gaacaggagg ctgtccggaa tgaggtaggt caggaatacg ccggttgcga gcgtcgccga
     2461 accccagaac ttcatccgcg ccagcgcgaa cgccgccggc acgctgatca gcatggtgac
     2521 ggcgacgaca atgatcgaga cccaggccga gttccagaag aagcggagga actggttcga
     2581 agtcaaaagc gcgatgtagt tttccagcgt cggatggtac acccaccacg ggttggtggc
     2641 cgccgaaatt tccgcgctgc tcttcagcga ggtgatcagc atgtagatcg gcgggaccag
     2701 cgaaaagatc gcaaacagcg cgaggaagaa ataggaccag cgcagcgccc agacccgatc
     2761 acggctcatg ctgccgtatt tgaccttgcg gcggggacct gccttgtcga ttgcgagcga
     2821 gctcatcagg cttcattccc gcgtttggag atgtcgcgga ggataaagac ggcggcaatc
     2881 gcgaggatcg gcgccataaa cagcgagaca ctggcgccga gcggaatgtc gctgccctcg
     2941 atgccgacgc ggaacgccca cgtcgcaaat atgtgggtat ggtccagcgg tccgccggcc
     3001 gtcaggatgc gcgttatgtc gaaattggcg aaggtaacga tcagcgagaa cagcgtggtg
     3061 atggcgatga tgttacgcat catcggcagc gtcacgtacc agatgcgttg ccaccagttg
     3121 gcgccgtcga tcgccgccgc ctcatagagc tgctcgggca ccgatttgag cgccgccaga
     3181 tacatgatca tgaaaaacgg cgcgccgtac cagacattga ccagaatgac cgagaagcgc
     3241 gcccagccga cctcgccgag ccaggaaatc ggtccaattc cgaagaagcc aagcgtgtag
     3301 ttgaaggcgc tataggaggg gtcgaacagc cacaaccacg ccagcgtgct catggccggc
     3361 ggtatcaccc agggcaccag cagcatgccg cgccatttgc gctgcttttt ggtgggaatg
     3421 ttgtgggcga aatgcgcgac gatgaatccg atcagcgcct tgaagatcac ggccgtgatg
     3481 gcgaagatgc aagattgctc caccaccatc cagaaggttt cgcgcttgaa caggaattcg
     3541 aaattgccga gcccgacaaa cttcgtcatc gccttgttca gtgtcgcgag atggaccgca
     3601 taaatcgccg gatagagcac cagcaacgca atcagcagga tcaggggtag cgtcatcaga
     3661 aacgcgatgg tcgatttccg ctgcagcgcg acctttaggc ttttccccgc ccgcctgctt
     3721 tcggcaacac gcgttgactg aaatacgaca tcaaccatgg tgcaattccc cggatcgcca
     3781 tttgagccgg tgggagaccg gcgcccgatc gcacgtcatg agaaccgatg cactgaggtc
     3841 ccgcaaggga ccgcagtgcc ttcaccactc atcagcttcg catgaagcct tcgcactcgc
     3901 cctcgaccca ggccagcgtc gattccatct tctcgccccg ggcataacgc aggcacatct
     3961 tggtcagtgt cgcctgggtg tagatctgct gggcaatttt cggcggcgcc ggagaagccg
     4021 cgatcgacag cttctgatgc ttgtagggat tcggatagtg gtagagcgta cccttcggcg
     4081 gctcttgctc ggcccaggct ttcagtgttg tcagtttttc gaatgccggc agatcatagc
     4141 caccgctggc attgaccagc cgttcaatcg agtccggttg cgaaagatgc gtcagcagac
     4201 tctttgcggc tgccttgttc ttggaaaaat tccaaacgct ccagaagaag gggaggtaag
     4261 gcgcaaaccg tcccttcggt ccagcgggga aaccgtgcgt ccagcactgc tcggccacct
     4321 gcggcgcgtc gcgcttggcg accgcccagg cgctcggcgg attcatgatc agcgcgccct
     4381 tgccggagat gagccacttg ttattggagg catcgtccca cgccgcgacg tcgggcggca
     4441 ggaatgagat cagctttttg tagtactcga gtgcctggcg caccgcatcg gtcttgaccg
     4501 tgaggtcgcc gttggcggtg acaagttctg caccgaacga ctggaagatc gcgcctgcgg
     4561 tatcgacact atcggtggtt tcgcccaatc cgatgccgaa gggatagccg cccttctggc
     4621 agtcttccgc cgcctttaga aatgtgtcca tcgtccaatt gtcggccttc ggcggagcgc
     4681 cggccggata catggcctgc acgtcaatat tggcatactt cttcatcagg tcgatgcgcg
     4741 agcacggccc cttgatctga ctgccgatgc aagcaggaac ggcgagccac tttccgccgg
     4801 cttggcctag atatttgacc gtgccgttca cgtcgccgtt ctctttgagg atcggctcca
     4861 tgatgtcgtt cattggctcc agcaactcgg caaaggcctg cggctgccag ggaccgccgg
     4921 acgccaggat gtcgtggccg gacttggctt gggcttcagt agtctgggtg aggagcagct
     4981 tgttgccctg gctcgtgatg taatcgatct ggacttcgac tttctccttc gcagcccact
     5041 cattcacgag gtcggtcgaa gtcttgttgg cgccgggaac ccagtgatcc cagaaaccga
     5101 tcgaaagact gccggccgcc cgtgcggaac ggacgtaagg agccgtgacc aacgccgccg
     5161 tagaggcggc agtcgcggca acgaattgtc gtcgtgtgag ctttttccgt gacatggcgt
     5221 tcccttcctg aaatcccgtt cttgttattt gccctgtggc cagatcaggc tgggcggaga
     5281 tttctcacca gcgatcagag cagaattgat ggcttctgtc gagaaatcag gtaggtaagc
     5341 gtctagaact aacgttgtgt tcaaaaagcg tgcagccaaa tcatgatcgg ccttgggacg
     5401 acgtgtcgcg cttggggatt ttgcgtcgca caccccgcgt cgcggccgcc ggcgcaattg
     5461 tgctgcagtc aagcttatcg gttttgcgcg gcaggctttt ctcttcgtgg acggcgaagc
     5521 cgggtggtgg acgagttccg cgcggtcgcg atagaatcca gatcacgcag cgcacccgac
     5581 gttccccaaa attctgccga gtgaagtcca gatcgatcat ggagaccgaa caaatgacca
     5641 gcccgacatc gaacctgcgg acgcgattaa aggtcgcagc ggcgcttggg gtggccgcgc
     5701 tcataatcgt ccacgcgcca cgcatggtgg gcgcgcaagg attggtcaaa ggggtcgagc
     5761 aaggagctcg cgaaggcacc aaggccgcgg gccctgtcgg cggcgtcctc ggaggcgcaa
     5821 tcggcggggt ggtcggggta gtcactggcg tcaccgacgc gctgaccggc aaaagcgggc
     5881 agccggtggc caacgataag gccaacgaca agggcaaggc cgccaagacc gcaaaggcga
     5941 cgaccgctaa caatccgaag gcaggaaagc cgatccaggt gcttacgcag caaggtgcgc
     6001 cgcagctcac ggccgagcag atcgtcgcca acagcgacgc ctatatcgag cggatcaagg
     6061 ccgaactgaa cctgacgccg gagcaggaga agagctggtc aagcttctcc agcgcgatgc
     6121 actatctcgg acataatggc gccgaccgtc tcaacctgcg gattgagcgc gccaagcgcg
     6181 atccgcccga cgacatcatc gaacagatgc gcaacgaagc acagttcctg gtcgaccgcg
     6241 cccaggacca gcgcaacgtc gccgacgccg ccgagccgct ttatgcaagc ctcgacggcc
     6301 agcaaaaaca gaaattcatc gacgaaatgg ttcggttgag ccacgaacgg gggcttgact
     6361 aaaggaaccc aaagcccgaa aggcacaatg gtcggcccgc cgaatagccg agtgcgcgtg
     6421 ccctggggta ggttaaaggc ccggcacccg gctatcctgc cgcagcggcg cggcgcttcc
     6481 actcgccatt caatgttgaa gaaaagcgga cggattcccg ccctatacgt ggcttggagg
     6541 tgaccttgtc gcgaagtgtg gccgatcacc ggacaaacgg cgtcagccgg acttgcgctg
     6601 gccgatgttc tctcccgatc gccgtgatcg ttcaggcctt cgggaagttc aattccacgc
     6661 cgacgccgtc aggatcgtaa aggaaaaact gtgtgtcgcc ggtgcgcggc acgatctgtt
     6721 cccggaactt gacgctgttg gcgatcaggc gcttccgcac gccatcgaca tcggtcgcgg
     6781 caaaggcgat gtggtcgagg cggccggtgt cctcaaatct cttatcggtg ccgcgcacca
     6841 cgatgccttc gcgcggcttg cgcgtgccca tcagatgcac cgtggcgttt ccgccggaat
     6901 agagccagta accggggaaa tcgaggggcg ggcggtcgcc gacctcaagt ccgagcacgt
     6961 tgacgtaaaa attcttgcta cgttcgaggt cctgcggctc gatcgtaaaa tgctgaagtc
     7021 cgccaagcgg catggttgct ctccctgttg atccgacccg attgctgact agacccaagt
     7081 aaggtcggcc tcggcgccca tcatccaggc gccgacggtt tggaaatggc tggcgcgacc
     7141 ctgcagttgc tgggtcacgg tgtggatatc gcggaagcgg cgctccaggg gatgatcatc
     7201 gaagatcgag gtggcgccgg ccgcatgata ggcgaggtcg accgcctcgc gcgccttgtg
     7261 gatcgcattg gtcgcggcca tgcgcacggt gatgcgctgg gcgaccgtga tcatggcgcc
     7321 ggcacaaaga tgcttccaga tctcggccat cgactgcagc acaaagccgc gcgctgcgcg
     7381 cagattgact tcggcctgcg ccagacccga ctgcaccacg gcattctcgc ggatcggagt
     7441 cctgcccccg cgaatgacct tgtccctggc cagaacgaca aaacagtcga gcgcgccgcg
     7501 ggcaatgccg caagccacgc ccgcaaagcc gacctggtag caggtgccgt tgcccatgcg
     7561 gtagagcggg ccgctctcgc ggcactcgcg gtcgaattcg cggataatcg agtgatcggc
     7621 gcgaacgaaa aagtcgttta atgcgaactg atcgcttgcc gtgccgcgca ggccgaccgt
     7681 gttccagatg tcggtccatt cgacgtcttc gctgcgtacc agcatggtgc gttctgcctg
     7741 gacgccattg tcctcgaggc gtggcgagcc gtcggctttg aagatcgggc agtgcgcacc
     7801 gagccaggtg gcgtggcggc cgccggaggc aaacgcccag acgccggtga ccctgtagcc
     7861 gccctcgcat tcgatcgcct tgaccttcgg
//
LOCUS       DATLJT010001116         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3065357,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001116 DATLJT010000000
VERSION     DATLJT010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3065357"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VM910_25755"
     CDS             <1..980
                     /locus_tag="VM910_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65962.1"
                     /translation="TTLLRRRFLHLAAGAVALPALARIAHGQAYPSRPVRIIVPFAPA
                     GPNDIIARALGQWLGERLGQPFVIENRPGAASNVGTEAVVNAAPDGYTVLMVSSPHAI
                     NATLYDKLHFEFRRDIAPVAAIMRVPNVMVVDRTFPARSVPDFISYAKANPDQLNFAS
                     SGIGASNHMSGELFKIMTGIAMTHVPYRSSGPALADLLGGRVQVMFDSIASTIEHIKA
                     DRLRALAVTSAPRSTLIPDTPVMSDFVPGYDVNNWFGLGMPSQTPAEVVDRLNTAVNE
                     GLVDPILKARLAGMGGTPLPGSPSDFGKLIADETEKWAKVIRTANIKPA"
     gene            complement(994..1959)
                     /locus_tag="VM910_25760"
     CDS             complement(994..1959)
                     /locus_tag="VM910_25760"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI65963.1"
                     /translation="MLRFMIALVVGLAASGVTAASAQTYPQRPVKFILPFGPAAGTDI
                     TARLLAEKLAARWGKPVVIENRPGGDGLVAINAFTSANDEHTLLFVPASTFTAHPYVH
                     EKLPYDAERDLLPITNVTTIVIALVAPASLNVKTLGEFIALARAKPDTLNVSAAAGNS
                     DLILSAFIKTQGLPVSRVPYRDIQQAPSDLAEARIHLLMSSYATMLPLVQAGKLRILA
                     VTSRKRVAIATDTPTVAEAGYPYLGLDGLIGMYGPRGMSMALRESIAADVRAVVEADP
                     TIASRLAQTGQVVDLRGPHEFAAGIKEIRDQLAAIAQSLGIKAAQ"
BASE COUNT          394 a          657 c          658 g          410 t
ORIGIN      
        1 gcacgacact tcttcgccgc cgattcctgc atctggccgc cggcgctgtc gcgctacctg
       61 ctctcgcgcg gatcgcgcac ggacaagcct atccgtctcg gccggtgcgg atcatcgtcc
      121 cgtttgctcc cgccggcccc aacgacatca ttgcccgcgc gttgggacaa tggctggggg
      181 aacggcttgg ccagcctttt gtcatcgaga accggcccgg cgcggcaagc aacgtcggca
      241 cggaggcagt cgtgaatgcg gcacccgacg ggtacaccgt cttgatggta tcgtcgccgc
      301 acgcgatcaa cgcgacgctc tacgacaagc tccatttcga gttcaggcgc gacatcgcgc
      361 cggtcgccgc gatcatgcgc gtgccgaatg tgatggtggt cgaccggacc tttccggctc
      421 gatcggtacc cgatttcatc agctatgcca aagccaaccc ggaccagctc aacttcgcgt
      481 cgtcagggat cggcgcgtcc aaccacatgt ccggggaatt gttcaagatc atgaccggga
      541 tcgcgatgac gcacgttccc tatcgcagtt ccgggcctgc actggccgat cttttaggcg
      601 gccgggtgca ggtgatgttc gactcgatcg cctccacgat agagcatatc aaagccgata
      661 ggctgcgcgc gttggcggtg acatcggccc cgcgatcaac ccttattcca gatacgcccg
      721 tcatgagcga tttcgttccc ggctatgacg tcaacaactg gtttgggctt ggcatgccga
      781 gccagacgcc ggcggaggtc gtcgacaggc tcaataccgc cgtgaacgaa ggcctcgtgg
      841 atcctatctt gaaggcgcgc ttagcgggca tgggaggaac cccattgccc ggctcgccct
      901 cggatttcgg caagctcatt gctgacgaaa ccgaaaagtg ggccaaggtc atccgcactg
      961 cgaatattaa gccggcatga tgcgcctgca ctgtcactgt gcggccttga tcccgagact
     1021 ttgcgcgatc gcggcgagct ggtcgcggat ctccttaatg ccggcggcga actcgtgcgg
     1081 gccacgcaaa tcgacgactt ggccggtctg cgccagccgg cttgcaatcg tcggatcggc
     1141 ctcgactacg gcgcggacgt cggcggcaat gctctcgcgg agcgccatcg acattccgcg
     1201 cggcccgtac atgccgatca aaccgtctag tccgagatac gggtaccctg cttcggcgac
     1261 ggttggagtg tcggtggcga ttgcgacccg cttgcggctg gtcaccgcca agatcctaag
     1321 ctttccggcc tgcaccagcg gaagcatggt cgcgtaggat gacattaaaa gatggatgcg
     1381 tgcttcggcc aggtcgctcg gcgcttgctg gatgtcgcgg tacggcaccc gggacacggg
     1441 taagccctgg gtcttgatga aagccgacaa gatcaggtcg gaattgccgg cggcggccga
     1501 cacgttgagc gtatcgggct ttgcgcgggc cagcgcaatg aactcgccga gcgtcttgac
     1561 gttcagagat gccggcgcca caagcgcaat cacaatggtc gtgacgttcg tgatcggaag
     1621 caggtcgcgc tccgcatcgt acggtagttt ttcgtgcaca tatgggtgtg ccgtgaaagt
     1681 cgaagccggc acgaacaaaa gcgtgtgctc gtcgtttgcg ctggtgaatg cgttgatggc
     1741 gacgaggccg tcgccgcccg gccggttctc gatcaccacc ggcttgcccc agcgcgctgc
     1801 gagcttttct gcgagcagcc gggcggtgat gtcggtcccg gcggcgggac caaacggcag
     1861 gatgaacttg accgggcgtt gcggataggt ctgcgcagag gccgcagtca cgccactggc
     1921 ggccaagccg accacgagcg cgatcatgaa ccgcaacacg caacgcctcc cgtctaacaa
     1981 cgacaaaaac tcagcatcac agtgacactt ctcagcaagg cggcggagga gcgtcgacca
     2041 attttggatt gatcgctttc aggccgtctg gccaagtcaa ttgattaaag tcaatctttt
     2101 gctcggtcat tttctcggc
//
LOCUS       DATLJT010001117         3633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_308724,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001117 DATLJT010000000
VERSION     DATLJT010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308724"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VM910_25765"
     CDS             <1..1343
                     /locus_tag="VM910_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HVI65964.1"
                     /translation="AFDGKAWGGMDVRSRARLVNRLADAFEANLEQLYRLETLNNGRP
                     VNETRAQLSRLPDFFRYFAGLALSRRDAVIPVEGSYLNYTLRTPIGIVANCTPFNHPL
                     MILCKSLAAVLASGCVTVVKPSEYTPLTTLKLARIFTEAGLPPGVFNVVLGLGQSTGK
                     MLAEHPDINKLVLTGGTEAGRIAGAAAAKVFAHQTLELGGKTPVMVFEDFDVDRAVNY
                     AAFGAFIGAGQTCVCASRHLVQASIYDEFVEKLKAKTESIRIGDPFDPSTQLGPVISA
                     RQRDRVLSYCGFGREDGARLLTGGAGAKIPGHENGYFVQPTVFCDVTSNMRIFQEEVF
                     GPFTSVTRFADEADALRLANNSPFGLAAAIRTRDVARAHRVAAAVKAGIVWLNDHHRL
                     DPASPWGGVDDSGVGREFGIESFDDHFNVKSVMVATHDQPFDWYRDTAGQRRLN"
     gene            1418..2761
                     /locus_tag="VM910_25770"
     CDS             1418..2761
                     /locus_tag="VM910_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI65965.1"
                     /translation="MALTVNAGARLDRLPISSFHYRIFWLVGAGMFFDGYDLYVAGGV
                     LASAIQTKFSTAPQNLQFISLTFVGMTLGALITGFVGDKFGRRFTYQINLLIFGLASI
                     AAAFAQDMNQLIACRFVQGLGLGAEIVVGYSTLTEFVPPRTRGRWLSMMAFLVVAGFP
                     ATALLGYLIIPTWGWRPMFVIAGAGSLIVWYLRKNLPESPRWLESQGRTAEAESLMQA
                     IETEAAGGKSLPPVVVQAPVAQVAASDMLKPPLLQRLIVGCWVLITINTLIFGFVIFL
                     PQFFLRQGLTITNSLAYTLVLSAGSLVGCALGAYSSDAIGRRFSIIGASIVTIVAGYI
                     YARFNAASDPAIVLTIGFVLIVAIYVQTAILFGVYTPELFPTEIRLRANGICNTFGRG
                     ATVVSPFIVGALMASYNLPGVVWLMIGLVVVQIVVVWAWGVEPRNRGLEDVAAKA"
     gene            complement(2846..3496)
                     /locus_tag="VM910_25775"
     CDS             complement(2846..3496)
                     /locus_tag="VM910_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HVI65966.1"
                     /translation="MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNYIPDGMDVSL
                     QSENGMLGMGPFPYEGEEDPDLINAGKQTVSELPSTSYFSSSDSFGMIRGGHMDLSIL
                     GAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGAKLLKKCTLP
                     LTGERVVDMVVTDLAVFTIDKHGNDGMALIELADGVTLDEVRANTEAEFRVALKNA"
     gene            complement(3496..>3633)
                     /locus_tag="VM910_25780"
     CDS             complement(3496..>3633)
                     /locus_tag="VM910_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HVI65967.1"
                     /translation="PDHIHTPGIFVKRMVEVGTAKKRIEFRNTRPRPANAPAAATGVE
                     I"
BASE COUNT          609 a         1169 c         1134 g          721 t
ORIGIN      
        1 cagccttcga cggcaaggcc tggggcggca tggacgtccg atcgcgggcc aggctcgtca
       61 atcggctggc ggatgcgttc gaggccaatc tcgagcagct ctatcgcctc gaaacgctca
      121 acaatggacg gccggtcaac gagacgcgcg cgcagttgtc gcggctgccc gacttctttc
      181 gctatttcgc gggcctcgcg ctgtcgcggc gcgacgcggt gatcccggtc gaaggatcct
      241 acttgaacta cacgctgcgc acgccgatcg ggatcgtcgc caactgcacg ccgttcaatc
      301 atcccttgat gatcctgtgc aagtcgctcg ccgcggtgct ggcctcgggc tgcgtcaccg
      361 tggtcaagcc gtcggaatac acgccgctga cgacgctgaa gctcgcaagg attttcaccg
      421 aagcgggact cccgcccggc gtattcaacg tcgtgctggg cctcggtcag agcaccggaa
      481 aaatgctcgc cgaacacccc gacatcaaca agctggtctt gaccggcggc accgaggccg
      541 gtcgcatcgc cggcgccgcc gccgcaaaag tgttcgcaca tcagacgctg gaactcggcg
      601 gcaagacacc ggtgatggtg tttgaggatt tcgacgtcga tcgtgcggtg aattacgcgg
      661 ccttcggcgc ctttatcggc gcggggcaaa cctgcgtctg cgcctcgcgc cacctggtgc
      721 aggcctcgat ctatgacgag ttcgtcgaga agctgaaggc gaagaccgag agcatccgca
      781 tcggcgatcc cttcgacccc tcgactcaac tggggccggt gatctcggca cggcagcgcg
      841 accgcgtgct ctcatactgc ggtttcggcc gcgaggatgg cgcgcgtctc ctgaccggcg
      901 gcgcgggcgc aaaaatcccg ggccacgaga acggctactt cgtccagcct accgtgtttt
      961 gcgacgtgac aagcaacatg cgaatcttcc aggaggaggt gttcggaccg ttcacctcgg
     1021 tgacgcgctt tgccgacgag gcggacgcgc tgcggcttgc aaacaattcg ccattcgggc
     1081 tcgcagcggc gatccggacc cgcgacgtgg cacgtgccca tcgcgtggca gcggccgtga
     1141 aggccggcat cgtctggctc aacgatcatc accgcctcga tccggcctcg ccctggggcg
     1201 gggtcgatga ctccggcgtc ggacgcgagt tcggcatcga gagtttcgac gaccatttca
     1261 acgtcaagag cgtgatggtc gcaacgcacg atcagccgtt cgactggtat cgcgacacgg
     1321 cgggccagcg gcgattgaac tgaacgaaca acgagcggtt acaacggccg gcgaagccgg
     1381 ccgccgcatt ggcgcatcaa aaagggggaa acttcgtatg gctcttacag taaacgccgg
     1441 cgcgcggctc gaccgcctgc cgatctcatc ctttcactac cggattttct ggctggtcgg
     1501 cgccgggatg tttttcgacg gatacgacct ctatgtcgcc ggtggcgtgc tggcgtccgc
     1561 gatccagacc aaattctcga ccgccccgca gaatctgcaa ttcatctcgc tgacttttgt
     1621 cggcatgacg ctgggagcgc tgatcaccgg atttgtcggc gacaaattcg gccggcggtt
     1681 cacctatcag atcaatttac tgatcttcgg gctcgcgtcg attgctgcgg cctttgcgca
     1741 ggacatgaat caactcatcg cctgccgctt cgtgcagggc ctcggccttg gtgcggaaat
     1801 cgtggtcggc tattcgacgc tgaccgaatt cgttccgccg agaacgcgcg ggcgctggct
     1861 gtcgatgatg gcgttcctcg tggtggccgg atttccggca actgccctgc tcggctatct
     1921 gatcataccg acctggggat ggcgtccgat gttcgtcatt gcgggcgccg gctccctgat
     1981 cgtctggtat ctgcgcaaga atctgccgga atcgccgcgc tggcttgaat cgcaaggccg
     2041 caccgccgaa gccgaatccc tgatgcaggc catcgagacg gaagccgccg gcggcaagtc
     2101 gctgccgccc gtcgtggtgc aggcgccggt cgcccaggtc gccgcaagcg acatgctcaa
     2161 gccaccgctc ttgcagcgct tgatcgtcgg ctgctgggtc ctgatcacca tcaacacgct
     2221 gatcttcggg ttcgtgatct tcctgccgca gttcttcctg cgccaggggc tgaccatcac
     2281 caattcgctg gcctatacgc tggttctttc ggccggatcg ctggtcggct gcgcgctcgg
     2341 cgcctactcg tccgacgcga tcgggcgacg cttcagcatc atcggcgcat ccatcgtgac
     2401 catcgtggcc ggctacatct atgcccgttt caatgcagcc tccgatccgg cgatcgtgct
     2461 gaccatcgga tttgtcctga tcgtcgcaat ctatgtgcag accgcgatcc tgttcggcgt
     2521 ctatacgccg gaactgttcc cgacggagat caggctgcgg gccaacggca tctgcaatac
     2581 gttcggacgg ggcgcaacgg tcgtctcgcc cttcatcgtc ggcgcgctga tggcatccta
     2641 caacctgccc ggcgtggtgt ggctgatgat cggccttgtg gtggttcaga tcgtggtggt
     2701 gtgggcctgg ggcgtggaac cgcgcaatcg cggcctggag gacgtcgccg ccaaagcgtg
     2761 accgcacgcg gctgccatcg ggccgcgctt ggcacggacc tcttggcaat ggcgaaacag
     2821 tcggccggat cttgcgtggc ggagcctacg cgttcttcaa cgcgacgcgg aactcggcct
     2881 cggtattggc cctcacctcg tccagcgtga cgccatcggc gagttcgatc agcgccatgc
     2941 cgtcgttgcc gtgcttgtcg atggtgaaga ccgcgagatc cgtgaccacc atgtcgacca
     3001 cgcgctcgcc ggtcagcggc agcgtgcact tcttcagaag ttttgccccg tccttggcgg
     3061 aatgctccat caccacgacg acgcgcttga cgccggcgac gagatccatc gccccgccca
     3121 tgcccttcac catcttgccg gggatcatcc aattggcgag atcgccgttc tgggccacct
     3181 gcatcgcgcc gaggatggaa aggtccatgt gcccgccgcg gatcatgccg aaggaatcgg
     3241 agctcgaaaa atagctggtc gacggcagtt cgctcaccgt ctgcttgcca gcattgatga
     3301 gatccggatc ttcctcgccc tcatagggaa acgggcccat gccgagcatg ccgttctcgc
     3361 tctgcaggct cacatccatg ccgtcaggaa tgtaattgga gaccagcgtc ggaatgccga
     3421 tgccgaggtt gacatagtag ccgtcgcgaa gctctttcgc ggcgcgggcg gccatctgct
     3481 cgcgggtcca tgccatcaga tttctactcc ggttgctgcc gcgggcgcat ttgccggacg
     3541 cggacgggtg ttgcggaact cgatgcgctt cttggcggtg ccgacttcga ccatgcgctt
     3601 cacgaatatg ccgggcgtgt ggatatggtc cgg
//
LOCUS       DATLJT010001118         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_309145,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001118 DATLJT010000000
VERSION     DATLJT010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309145"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VM910_25785"
     CDS             <1..523
                     /locus_tag="VM910_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI65968.1"
                     /translation="FSGGQRQRICIARALALGPRLIVCDEPVSALDVSIQAQVINLLI
                     DLQREHGFSYLFIAHDLAVVAHISHRVAVMYLGRIVEIADKSELFRNPRHPYTQALLA
                     SVPVADPRSKRLKPLVDGDVPSPVDPPSGCAFHTRCRYAMDRCKVERPLLRDAGERHQ
                     VACLLNEGTGRPA"
     gene            complement(548..805)
                     /locus_tag="VM910_25790"
     CDS             complement(548..805)
                     /locus_tag="VM910_25790"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HVI65969.1"
                     /translation="MTALSTGRAADQTMTLALGSGSVFTLERPFDTVLIDQADVVDIH
                     CQGSRSVILKALNPGTVNVVFVDAQSVAIANVRIVVRDAQI"
     gene            complement(997..>2337)
                     /locus_tag="VM910_25795"
     CDS             complement(997..>2337)
                     /locus_tag="VM910_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVI65970.1"
                     /translation="AIANPRHIEIQVFGDRYGNAIHLGERDCSVQRRHQKLIEEAPSP
                     AVSPKLRQRMGEVAVAAIRSLRYEGAGTLEFLLDESGEFYFMEMNTRLQVEHPVTEAI
                     TGLDLVELQLRIARGEPLSLVQEDIRFSGHAVEVRLCSEDAGHDFMPQSGQMTRWQMP
                     EGVRVEHALQSGSEISPFYDSMIAKIISHGADREEARGRLICALEQAIAFGVTTNQAF
                     LLACLRSPAFAHGEATTAFIDLYKQQLLAKHTPSDTALAALLLYITHPHAPHLRHGRS
                     IAATFPIPMRIEIDREVHDAEILREREGGYVVSRNGDTHRFEMDELAEGVIRFREDGV
                     MESIKFHRAGDLLYFLHRGAPHAVRDLTLAAPAAAGTSGGDGKVRAAMNGRVVAVLVK
                     KGDRVTPGQPVLTLEAMKMEHVHTAGVAGTIVEIDANEGEQVTTGQIVVEIAAG"
BASE COUNT          432 a          762 c          713 g          430 t
ORIGIN      
        1 gttttccggc ggccagcgcc agcgcatctg cattgcgcgg gcgctcgcac tcgggccgcg
       61 cctgatcgtg tgcgacgagc cggtgtcggc gctcgacgtc tcgatccagg cgcaggtgat
      121 caatctcttg atcgacctgc agcgcgagca cggcttttcc tatctcttca tcgcgcacga
      181 tctggcggtg gtcgcccata tcagccaccg cgtcgccgtg atgtatctcg gccgcatcgt
      241 cgagatcgcc gacaagagcg aattgttcag gaatccgcgc cacccctata cgcaggccct
      301 actggcatcc gtgcctgtcg cggacccccg atcaaagcgt ttgaagccgc tggtcgatgg
      361 cgacgtgccg agcccggtcg atccaccctc gggctgcgcc ttccacactc gctgccgcta
      421 cgccatggat cgctgcaagg tcgagcggcc tctcttgcgg gacgccggcg agcgacatca
      481 ggtggcgtgc ctgctgaatg agggaaccgg gcggcctgcg tgaccgtgag ctcgtcgccc
      541 gtccggatca gatctgcgcg tcgcgaacga cgatcctgac attggcaatc gcgacgctct
      601 gtgcgtccac gaacacgaca ttcaccgttc ccggattgag cgccttaagg atgaccgaac
      661 ggctgccctg gcaatgaata tcgacgacat ccgcctggtc gatcagaaca gtatcgaagg
      721 gcctttccag cgtgaagacc gaccccgaac cgagcgcaag ggtcatggtt tggtccgcgg
      781 cgcggccggt ggacagcgca gtcaagaaaa ggccaactcc aagcgcaaaa gcgcgaagcc
      841 gcgcgatcct catggtcacc ccgctgcgtc tgctgtcgat agcctgagga tagcgccccc
      901 cgaagccggg caaacgtgaa gcaattcaca caccgacgat atctgtgaca gtttgacgcg
      961 agggcgggga tgcgcgagcc caccgctctg acgttgctag ccggccgcga tctcgaccac
     1021 gatttgtccc gtggtgacct gctcgccttc attggcgtcg atttcgacga tggtgccggc
     1081 gacgcctgcc gtgtgcacgt gctccatctt catggcttcc agcgtcaata cgggctgacc
     1141 gggagtgacg cgatcaccct ttttcacgag caccgcgacg acgcggccgt tcatcgcggc
     1201 gcgcaccttg ccgtcgccgc cgcttgtccc cgccgctgcc ggggcggcga gcgtgagatc
     1261 gcgaacggcg tgcggcgcgc ctcgatgcaa gaaatacagc aggtcgccgg cgcgatggaa
     1321 tttgatgctc tccatcacgc catcttcccg gaagcggatg acgccctccg ccagttcgtc
     1381 catctcgaac cgatgcgtgt cgccattgcg gctcacgacg taaccgcctt cacgctccct
     1441 cagaatttct gcatcatgaa cctcgcgatc gatttcgatc cgcatgggaa tcggaaacgt
     1501 cgcggcaatg gagcgcccgt gccttaagtg cggcgcatgc ggatgagtga tatagagcag
     1561 caaggcagcc agcgccgtgt ccgatggcgt atgcttcgcc agcaactgct gtttatacag
     1621 gtcgatgaag gccgtggtcg cctcgccgtg cgcgaaggca ggagaccgca aacaggccaa
     1681 gagaaaagcc tgattggtgg tgacgccaaa agctatggcc tgctcgagcg cgcagatcag
     1741 tctgccgcgc gcttcctcgc gatcggcgcc gtggctgatg atcttggcaa tcatggaatc
     1801 gtagaatggc gaaatctcgg aacccgactg caaggcatgt tccacgcgaa cgccctcggg
     1861 catctgccag cgcgtcatct ggcccgactg cggcatgaag tcatggccgg catcttcgga
     1921 gcagagccgg acctcgacgg catggccgga gaagcggata tcctcctgca ccaaagacag
     1981 cggttcgccg cgcgcaatcc gcaattgcag ctcgacaagg tcaagcccgg tgattgcctc
     2041 ggtgacggga tgctcgacct gcagccgcgt attcatttcc atgaagtaga actcgccgct
     2101 ctcgtcgagc aggaattcga gcgtgcccgc accctcgtag cgaagcgatc ggatcgccgc
     2161 caccgcaact tcacccatcc gctgtcgcag cttgggcgag accgccggtg acggcgcctc
     2221 ttcgatcagc ttctggtgcc ggcgctgcac cgagcaatcg cgctcgccga gatgaatcgc
     2281 gttgccgtag cggtcgccga acacctgaat ttcgatgtgc cgtggatttg cgatggc
//
LOCUS       DATLJT010001119         1502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_78646,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001119 DATLJT010000000
VERSION     DATLJT010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78646"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VM910_25800"
     CDS             <1..231
                     /locus_tag="VM910_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HVI65971.1"
                     /translation="PEMARGRGVRLQKYTSASLSDVTTFDSKEGLAWKDSAGRDQSMT
                     MKELSDWRGNRADAGRLAHGLPKSNKFGKPVE"
     gene            complement(264..1232)
                     /locus_tag="VM910_25805"
     CDS             complement(264..1232)
                     /locus_tag="VM910_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65972.1"
                     /translation="MALGKFVSARELAIVMTRCARIDRRPNLRMLLIALFAIPCGAAL
                     AAEQQGRSQLPVARSVFAPVLAPAGTLVLMVPFNQGLIVAADGRQTVFNRIYCDGAGK
                     IIEPASPPRTIAVVTGNGVVVDYPHPVPDGEMCDYIKSAPRILDIEAVVKRFLETSDV
                     PLRSLKLEALVQLCLNELVKFDGTHPGLLVQFLGTTMFQVVLANYDVESSTTVVRAFY
                     GRIEKNGRVTFQIAQDKSYSPETDADWLMFGEADYVSQHVLGPEGKAFYDRSFRAHER
                     ASHLRGEPRGSDCTRHALARGGVQNDDRHPRADRNRRPIRSPGHRA"
     gene            1300..>1502
                     /locus_tag="VM910_25810"
     CDS             1300..>1502
                     /locus_tag="VM910_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65973.1"
                     /translation="MKRQIIATVFVTALAVSAAKVQDMRRVVTGLDDRNHAIVLFDSA
                     MPLKDIGPGIKATNFWITDGYPP"
BASE COUNT          298 a          468 c          461 g          275 t
ORIGIN      
        1 ccggagatgg cgcgtgggcg cggcgtgcgc ttgcagaaat atacgagcgc ctcgctctcg
       61 gatgtcacga ccttcgattc caaggagggc ctcgcctgga aggactcggc gggccgcgac
      121 cagagcatga ccatgaagga attgtccgac tggcgcggca accgcgccga tgccggccgt
      181 cttgcgcacg gcctgccgaa gtcgaacaag ttcggcaagc cggtcgaata gcgcgctatt
      241 ggccgatctt ttcgggcctt ggatcacgcc cgatggccag gacttcggat gggccgccga
      301 ttccggtcgg cgcggggatg acggtcgtcg ttttggacgc cgcctcgagc gagtgcgtgg
      361 cgagtgcaat cgcttcctcg cggctcacct cggagatggg atgccctttc atgcgctcga
      421 aaagaccggt cgtagaacgc cttgccctcg gggccaagga cgtgctggct gacatagtcc
      481 gcttcgccga acatcagcca gtcagcatcg gtttcgggtg aataggactt gtcctgcgca
      541 atctgaaacg tgaccctgcc attcttttcg atgcgcccgt agaaggccct cacgacggtc
      601 gtgctgctct cgacgtcgta gttcgcgagc acgacctgga acatcgtggt tcccaaaaac
      661 tgaaccaaca gccctggatg cgtcccgtcg aacttgacca attcgttcag gcatagctgc
      721 accagcgcct cgagcttgag gcttcgcagc ggcacgtcgc tggtctcgag aaatctcttc
      781 accaccgcct cgatatccag aattcgcggc gcgctcttga tgtagtcgca catctcgcca
      841 tcgggcaccg gatgcggata gtcgaccaca acgccattgc cggtcacgac cgcgatcgtg
      901 cgaggcgggc ttgccggctc gatgatcttt ccggcgccgt cgcaatagat cctgttgaac
      961 acggtctggc ggccgtcggc ggcaacgatc agcccttgat tgaagggcac catcaacacc
     1021 aacgtgcccg cgggcgccag aaccggagca aacacggacc gcgcgaccgg aagctgcgat
     1081 cggccctgct gctccgcggc aagagccgca ccgcagggga tcgcgaacag cgcgatcagc
     1141 agcatgcgaa ggttcgggcg gcgatcgatg cgagcgcagc gggtcatgac gatcgcaagc
     1201 tcacgcgcag aaacgaattt gccgagggcc attgatgtcg cacccgcctt acgattgtat
     1261 cgccggcgat gggagaaacg ccaacttcgc aacgcaacca tgaaacgcca aatcattgca
     1321 acggtcttcg tgaccgcgct tgccgtatcc gctgccaagg ttcaggacat gcgccgtgtg
     1381 gtcacgggcc tcgatgacag gaaccatgcg atcgttctgt tcgacagcgc aatgccattg
     1441 aaggatattg ggcccggcat caaggcgacg aatttctgga ttaccgacgg ctatccgccg
     1501 aa
//
LOCUS       DATLJT010001120         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1457904,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001120 DATLJT010000000
VERSION     DATLJT010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457904"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /gene="paaI"
                     /locus_tag="VM910_25815"
     CDS             complement(<1..359)
                     /gene="paaI"
                     /locus_tag="VM910_25815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HVI65974.1"
                     /translation="MNVKATLSPDDLARACADAMWKEDDASSGLGMKIVEIAPGRATL
                     TMTVRPDMVNGQRIAHGGFIFTLADSAFAFACNSHNARAVAAQGHITFIKPGKLGDVL
                     VATAREVSRRGRSGIYDV"
     gene            complement(456..1535)
                     /gene="paaE"
                     /locus_tag="VM910_25820"
     CDS             complement(456..1535)
                     /gene="paaE"
                     /locus_tag="VM910_25820"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HVI65975.1"
                     /translation="MSHTPRFHRLAVNDLRRETADAVSLTFAIPQELAGDYRFAPGQY
                     LTLRTMMDGEEVRRSYSICSGPDDGELRIAVKKVDGGAFSSWAADELKAGDELDVMTP
                     TGRFGVQPAPEEARTHVGFAAGSGITPILSIVKGVLAREPKSDFFLFYGNRSSEGMLF
                     REALEELKDRFLERLSVFHVISGEEQDLPILHGRLDGEKVTVLLRSLVPAESIDHVFI
                     CGPTGMSEEIEKTCRDIGIAEERIHVERFVSGLGGRPRPKAPIPPTAPPKAFASLIID
                     GKRRDVPVAEGEAILDAALRAGVDLPFACKGGMCSTCRAKLVEGKAEMELNYSLEPWE
                     LKAGFVLTCQAKPVSEKVVVDYDHV"
     gene            complement(1604..2107)
                     /gene="paaD"
                     /locus_tag="VM910_25825"
     CDS             complement(1604..2107)
                     /gene="paaD"
                     /locus_tag="VM910_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HVI65976.1"
                     /translation="MVTAVLSDTDLRRRAWNAAASVVDPEIPVLTIADLGVLREVSVS
                     DGHVEVAITPTYSGCPAMNMIALEIELALEREGFHKPSIRTVLSPAWTTDWMSEDGRR
                     KLREYGIAPPLAASSRRALFGVQEVACPQCGSKDTEQLAEFGSTSCKALWRCKSCREP
                     FDYFKCH"
     gene            complement(2101..2877)
                     /gene="paaC"
                     /locus_tag="VM910_25830"
     CDS             complement(2101..2877)
                     /gene="paaC"
                     /locus_tag="VM910_25830"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HVI65977.1"
                     /translation="MAAPSIKVSETPLVLYVLRRADDALILGHRLSEWCGHAPMLEED
                     MALANMGLDLLGQARELYTYAAKVEGGGNDEDKFAYLRDVRQYRNLLLLEQPNGDFAR
                     TMVRQFFYSAFADLYWCAMMKSTDTTLAAIAAKSEKESAYHLRHSSEWMLRLGDGTDE
                     SHVRAQTAIDDLWSFTGEMFAVDDSERGLIDAGIAIDPAGLNPQWLQTVSSIVAEATL
                     ALPTSDWMQQGGRFGRHSEHLGHLLSDLQYLQRAFPGATW"
     gene            complement(2877..>2988)
                     /gene="paaB"
                     /locus_tag="VM910_25835"
     CDS             complement(2877..>2988)
                     /gene="paaB"
                     /locus_tag="VM910_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613353.1"
                     /note="with PaaBCDE catalyzes the hydroxylation of
                     phenylacetyl-CoA; involved in phenylacetate degradation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HVI65978.1"
                     /translation="ITASDPSEKGMMFEPAESKIYRHPTFYDVPEEVGHM"
BASE COUNT          551 a          939 c          939 g          559 t
ORIGIN      
        1 acgtcgtaga tgccggatcg gccgcgccgg gaaacctcgc gcgcggtcgc gaccaggacg
       61 tcgccgagct tgcccggctt gataaaggtg atgtggcctt gcgctgcgac cgcgcgcgcg
      121 ttgtggctgt tgcaggcaaa ggcgaaggcg gaatcggcca gcgtgaagat gaagccgcca
      181 tgggcgatgc gctgaccgtt gaccatgtcg ggcctgaccg tcatcgtgag cgtcgcgcgt
      241 ccgggtgcga tctcgacgat cttcatgcca agcccgctgg aggcgtcgtc ttccttccac
      301 atggcgtcgg cgcaagcgcg cgcgagatcg tcgggcgaaa gcgtggcctt gacgttcact
      361 ctcattgcct ccgtagcttc accttttcag ggatttcgaa agtcgggctc cagaccgcca
      421 tacaggcgcg gggcggtgtt tcttcggatc atgttctaga cgtgatcgta gtccaccacg
      481 actttttcgg aaacaggctt ggcctggcaa gtgagaacga acccggcttt cagctcccac
      541 ggctccagcg agtagttcag ctccatctcg gccttgcctt cgacgagctt cgcgcggcat
      601 gtcgagcaca ttccgccctt gcaggcgaac ggcaggtcga cgccggcgcg caatgcggcg
      661 tcgaggatgg cctcgccctc ggcaaccgga acgtcgcgcc gcttgccgtc gatgatcagc
      721 gaggcaaacg cctttggcgg ggccgttggg ggaattgggg ccttgggccg cggcctgccg
      781 ccgagcccgg agacgaagcg ctcgacatgg atgcgctcct ccgcgatgcc gatatcgcgg
      841 caggtctttt cgatctcctc gctcatgccg gtcgggccgc agataaagac gtgatcgatg
      901 ctctcggccg gcaccagcga acgcagcaga accgttacct tctcgccgtc cagccggccg
      961 tgcaggatcg gcaaatcctg ctcctcgccg gagatgacgt ggaacaccga cagcctctcg
     1021 agaaaccgat ccttcagctc ttccagagcc tcgcggaaca gcatgccttc gctactgcga
     1081 ttgccgtaga acaggaagaa gtcgctcttg ggttcgcgtg ccagcacgcc tttgacgatc
     1141 gagagaatcg gggtgatgcc gcttcccgcg gcaaagccga catgggtccg cgcctcctcc
     1201 ggcgcaggct gaacgccgaa acgcccggtg ggcgtcatca cgtcgagctc gtcgcccgcc
     1261 ttcaattcgt cggcggccca gctcgaaaat gcgccgccgt cgactttctt cacggcgatg
     1321 cgcaattcgc cgtcgtccgg accggaacag atcgagtagg accggcgcac ttcctcgcca
     1381 tccatcatgg tgcggagcgt cagatactga ccgggcgcaa agcggtagtc gccggcaagc
     1441 tcttgcggga tcgcaaaggt cagcgacacc gcgtccgcgg tttcgcggcg caaatcgttg
     1501 acggcaaggc gatggaaacg gggcgtgtga gacatcaatt gcttcccggc gtcattcgcg
     1561 agcgagaatt gcttcggagc tcacgctcct cgcaatgacg gtgttagtga cacttgaaat
     1621 agtcgaaggg ttcacggcag ctcttgcaac gccacagcgc cttgcaggag gtcgagccga
     1681 actcggcgag ttgctccgtg tctttcgagc cgcattgcgg acacgccacc tcttgcacgc
     1741 caaacagcgc acggcgggaa cttgcggcaa gcggcggcgc aatgccgtat tccctgagct
     1801 tgcggcggcc gtcctcgctc atccagtcgg tggtccaggc cggtgacagc acggtgcgga
     1861 tgcttggctt gtgaaagcct tcgcgttcca gcgcgagctc aatctccagc gcgatcatgt
     1921 tcatcgccgg acagcccgaa taggtcggcg tgattgcgac ctcgacatgg ccgtcgctga
     1981 cactgacctc gcgcaacacg ccgagatcgg cgatggtcag gaccggaatc tccggatcga
     2041 cgacgctcgc cgcagcgttc caggcgcgcc ggcgcagatc ggtatcgctg agaacagcgg
     2101 tcaccatgtc gcccccggaa atgcacgctg cagatattgc aggtcgctca ggagatggcc
     2161 gagatgttcg ctatggcggc caaagcgtcc gccctgctgc atccagtcgc tcgtcggcag
     2221 cgccagtgtc gcctcggcga cgatagagga aaccgtctgg agccattgcg ggttcaggcc
     2281 agccgggtcg atggcgatgc cggcatcgat caggccgcgc tcgctgtcat cgaccgcaaa
     2341 catctcgcca gtgaaggacc agaggtcgtc gattgcagtt tgcgcgcgga catgactctc
     2401 gtcggtgcca tcgccgagcc gcagcatcca ttcggaggaa tggcgcaagt gataggcgct
     2461 ttccttttcc gatttcgctg caatggcggc aagcgtcgtg tcggtcgact tcatcatcgc
     2521 gcaccaatag aggtcggcaa aggcggaata aaagaactgc cggaccatgg tgcgggcgaa
     2581 atcgccattc ggctgctcca gcagcaaaag attgcggtat tgcctgacgt cgcgaagata
     2641 ggcgaactta tcctcgtcgt tgcccccgcc ttcgaccttg gccgcatagg tatagagctc
     2701 gcgggcctga ccgagcagat cgaggcccat attggcgagt gccatatcct cttcgagcat
     2761 gggcgcatgc ccgcaccatt ccgaaagccg gtggccgagg atcagcgcat cgtcagcccg
     2821 ccgcagcacg taaagtacga gcggcgtctc ggaaacttta atcgacggtg cagccatcac
     2881 atatgcccga cttcttcggg tacgtcgtag aacgtcgggt ggcggtaaat cttggattcc
     2941 gccggctcga acatcatgcc cttctcggac gggtcggacg ccgtgatc
//
LOCUS       DATLJT010001121         5895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_78847,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001121 DATLJT010000000
VERSION     DATLJT010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78847"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VM910_25840"
     CDS             complement(<1..457)
                     /locus_tag="VM910_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65979.1"
                     /translation="MTVCTVCGCDPCRSPAFCASCATADARKPHSPPLLNIDSGNLPT
                     VALNLRDILANSGTLYNRGTPVRITLSSSDAVPIATPLTTHRVVRLAHEFSRPIKNGE
                     PHTLPDRVANMYLDMQGEWGLPKLAGICTTPILRDDGSVVSAEGYDLATG"
     gene            complement(454..615)
                     /locus_tag="VM910_25845"
     CDS             complement(454..615)
                     /locus_tag="VM910_25845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65980.1"
                     /translation="MTTTIPSTVDWREFWCAESVRLGFDWPSIIATHLTIVDQLRDAD
                     DADVRMASS"
     gene            complement(746..988)
                     /locus_tag="VM910_25850"
     CDS             complement(746..988)
                     /locus_tag="VM910_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65981.1"
                     /translation="MNQQEVIDELRERIARLEAANTRRRIYNQVQAAERLNMSVNKLR
                     ALHKQGLGPRRLSVSGRIWSYADEALEEYISTQSDN"
     gene            complement(1097..1576)
                     /locus_tag="VM910_25855"
     CDS             complement(1097..1576)
                     /locus_tag="VM910_25855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65982.1"
                     /translation="MSSPYPLLRPYYKGHSREEDEKLANELFYGTSTIVDKKTGRCRH
                     DYPDGLDERRAIEALQRLLLFSCGDLAPGILGGLVASLAPGGSFGRRLVFKPRKRKRP
                     AGSATDLQIALHVRSLHHRFGKKEAAVKQAMADFDLSRKTVFEAMKRIRAESPWLEV"
     gene            complement(1573..2712)
                     /locus_tag="VM910_25860"
     CDS             complement(1573..2712)
                     /locus_tag="VM910_25860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI65983.1"
                     /translation="MRLTDSSVAALKVPARKSEIIAFDDSLPGFGVRVRRGGSKRFIY
                     QYKLNGSNRRVTFKEANVKRARAAAQILAAKVTLGADPALEKEAAHDAAGDTFKRCLD
                     RYLARPQGKRRERTLQEIRRHLERNLAPLHRLHIKAIDRRRVADELMRLTEENGAVQS
                     NRTRSSLSAFLNWCIGEGYIDVNVAAQTNKHEEIARSRVLSDSELRAIWRALPEPGDY
                     RDLIRLLILTGQRLREIANLSWREVDLDKAIITLPPSRTKNHREHVVPLSAPALEILK
                     GRVCEERDLVFGNGEHGYSGFSKAKHQLDEKAKLKVPWVIHDIRRSVATGLAKLVQPH
                     IVECVLGHVGGFRAGVSGVYNLHAYEGEKRHALDLWGKHISGLVS"
     gene            complement(2929..3471)
                     /locus_tag="VM910_25865"
     CDS             complement(2929..3471)
                     /locus_tag="VM910_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HVI65984.1"
                     /translation="MAHPFFTIGHSTRPVREFVELLEGAQIGCVVDVRSVPRSRTNPQ
                     YNCDILPETLAQFQIGYEHIAALGGLRPRTRDVPPDVNAFWQNQSFHNYADYTLTPPF
                     HGGLARLRALGHERRCAIMCAEAVWWRCHRRIIADYLIAASETVFHILGKDRIEPARM
                     TDAAKPRPDGLLTYAAADAR"
     gene            complement(3489..3704)
                     /locus_tag="VM910_25870"
     CDS             complement(3489..3704)
                     /locus_tag="VM910_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HVI65985.1"
                     /translation="MKKRFKLHDHVSWNSEAGRVSGRIVKVHTKDVNYKGYVHHASKD
                     EPQYEIKSDKTDHVALHKGAALRLMRR"
     gene            complement(3775..3984)
                     /locus_tag="VM910_25875"
     CDS             complement(3775..3984)
                     /locus_tag="VM910_25875"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HVI65986.1"
                     /translation="MAGGNVPRATNVNFSINVGTVVPTSVRVVEVPSVIVDIHPEWRR
                     FWYFVVGDEIIIVDRGYKIVAILAV"
     gene            4048..4323
                     /locus_tag="VM910_25880"
     CDS             4048..4323
                     /locus_tag="VM910_25880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65987.1"
                     /translation="MLSLTLLGLPLLPGLALLTLLALRGLTLGLLSLLALRLRLLVLL
                     PLALGRLARGLPFLIALRLALLLSLHVLLRLPLHVVPALGLAARSGL"
     gene            complement(4759..>5895)
                     /locus_tag="VM910_25885"
     CDS             complement(4759..>5895)
                     /locus_tag="VM910_25885"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI65988.1"
                     /translation="AMAEDPAWVAQQAQDHAPAAPADAAIIDQLVVWVRYAFVAALLA
                     VLAARMARWYWQRRRGIVRLTYPNGRVVQIVRGVSVLEASRMAGIPHASVCGGRGRCS
                     TCRIRVDGAKEAIVPPAAEEVKVLHRVGAAPDVRLACQLRPHGDLRVTPLLPASAQAR
                     DGFSRPGYLHGAEREIVILFGDLRSFTQLSEKKLPYDLVFLLNRYFAETGHAVETAGG
                     RIDKFIGDGVMALFGLDSGVEAGCREALVAAKDMAERMETLNRALIHDIPEPLRIGIG
                     IHTGPAIVGEMGYGTAVSITAVGDSVNTASRIEALTKTYACELVISEAVAVRAGVDLG
                     DAPRHEIEIRGRVERLVVRTFASARDLPIPERRGRKKAASVAAV"
BASE COUNT         1027 a         1782 c         1844 g         1242 t
ORIGIN      
        1 cgcccgtcgc caaatcatat ccttcggcac tcacgaccga gccgtcgtcg cgcagaatcg
       61 gtgttgtgca gatcccggcg agcttgggta gtccccactc gccctgcatg tccaagtaca
      121 tgttcgcgac gcggtcgggc aacgtgtggg gttcaccatt tttgattggg cgtgaaaact
      181 cgtgcgccag cctgacaacg cggtgtgtcg tgagcggtgt ggcaatcggc acggcgtcgc
      241 tcgacgaaag tgtgatgcgt actggcgtgc ctcggttgta gagagtgcct gagttcgcaa
      301 gaatatcgcg caggttgagg gcgacggtcg gcaggttgcc cgagtcgata ttcagtaatg
      361 gcgggctgtg cggcttgcgg gcgtccgccg tggcacagct agcgcaaaag gcgggacttc
      421 ggcacgggtc acagccgcag acggtgcaga cagtcatgac gaagccatcc gcacatccgc
      481 gtcatccgcg tcgcgcaact gatcgacgat ggtgagatgc gtggcgatga tggatggcca
      541 gtcgaatccg agccgcaccg attcagcgca ccagaattca cgccagtcga ctgtcgaggg
      601 gatggtggtg gtcatggacg tttcagcttt caattgccgc tggcggcggg ggtcgaattg
      661 cgctagaacg tcagccttcg aacggacgat ctagctttgg ccgctttggg ggatcctcca
      721 gaatcccccg gcggcttctg ctttttcagt tgtcggattg cgtggagata tactcctcaa
      781 gcgcctcgtc ggcgtacgac caaattcttc cgctcacgct gaggcgtcta ggaccgagac
      841 cttgcttgtg cagggcgcgc agtttgttga cggacatgtt caggcgttcg gcggcctgaa
      901 cttggttgta gatccggcgg cgcgtgttcg cggcttcgag ccgggcgatg cgctcgcgca
      961 attcgtcaat gacttcttgc tgattcatga gggtatgctc cgctacgcgt tttggcgtga
     1021 cgggcaccta tttattacag agccgggaag gcaagaagcg aaaagcggtc acctataaat
     1081 gcaagattcg gtgactctag acttcgagcc acgggctttc ggccctgatg cgtttcatcg
     1141 cttcgaacac cgtttttcgc gataaatcga agtcggccat tgcttgtttt acggcagctt
     1201 cttttttccc aaagcgatga tgtagggacc ggacgtgcag ggctatctga agatccgtcg
     1261 cagagccggc tggccgcttt ctttttctcg gcttgaatac aagacgccgt ccaaagctgc
     1321 cgcctggagc gagcgaggcc accaatccac ctagtattcc gggggccaaa tctccgcagg
     1381 aaaataacag gagtcgctga agcgcctcga ttgcgcggcg ctcatctagg ccgtctggat
     1441 agtcatggcg acagcgaccg gtttttttat cgacgatagt cgacgttccg taaaacaatt
     1501 cgttcgcaag cttttcatct tcctcgcgac tgtgtccctt atagtagggg cgaagcaacg
     1561 gatatgggct gctcatgaca ccagcccgct gatgtgcttg ccccaaagat cgagcgcatg
     1621 acgtttctcg ccttcgtagg cgtggaggtt atagacgcca ctgactccag ctctgaagcc
     1681 gccgacatgg ccaagaacac attcgacgat gtgcggctgg acaagcttag cgagtccggt
     1741 cgctacgctt cgcctgatgt cgtggataac ccacgggact tttagcttcg ccttttcatc
     1801 gagctggtgc ttggcctttg aaaatccaga atagccatgc tcgccgttgc cgaagacgag
     1861 atcacgctcc tcgcacaccc tgcccttcag gatctccaat gccggcgcgg ataatggaac
     1921 aacatgctct cggtgatttt tcgtccggct tggcggcagg gtgatgatcg ccttgtcgag
     1981 gtcaacctcg cgccaggaca gattggcgat ctcgcgtagc ctttggccgg tcagaatcag
     2041 cagtcgaatg aggtcgcgat agtcgcctgg ttctggcaga gcgcgccaga ttgcccgcaa
     2101 ttccgagtcg gacagaacgc gactgcgggc aatttcttca tgcttattgg tctgtgccgc
     2161 gacattgaca tcgatatagc cctcgccaat gcaccaattg agaaaggccg acaggctcga
     2221 acgcgttcta ttggattgga cggcaccgtt ctcctcggtg agccgcatga gttcatcggc
     2281 aacccggcgg cggtctattg ccttgatgtg caggcggtgc aagggcgcga gattgcgttc
     2341 aagatgtctg cggatttcct gcaacgtgcg ctcgcgccgc tttccctgcg ggcgggcgag
     2401 atagcggtcg aggcatcgct tgaaggtgtc ccctgccgcg tcgtgggcgg cttctttctc
     2461 gagcgccgga tccgcgccca atgtcacctt ggcggcaaga atctgcgcag ccgccctcgc
     2521 ccgtttgacg ttcgcttcct tgaaggtgac gcggcgattg ctgccgttga gtttgtattg
     2581 gtagatgaat cgcttgctgc cgccgcggcg aacgcggaca ccaaagccgg gaagcgaatc
     2641 atcgaaggcg atgatttcgg acttgcgggc gggcactttg agcgccgcaa cgctggaatc
     2701 agtcagccgc aaagaaggtc ctcctgggaa tctgggaaga tgaactcggt attttggcac
     2761 gcgtaggtac gcgtcgagac tgagggtcct agccgtttgc tgggcaaaaa gcaagtgctg
     2821 ggaagcgcct acgcgtagtt acgcgtcacg ttttcggtct gggggactgg gggtcccgag
     2881 ttcaaatctc ggcgctccga ccaactcaaa gccaaaacca aaccgtgttc agcgggcgtc
     2941 tgcggcggcg taggttagca aaccgtccgg ccgaggcttt gccgcatcgg tcatgcgcgc
     3001 cggctcgatg cgatccttgc cgagaatgtg aaacacggtc tcgctggcgg cgatcaggta
     3061 gtccgcaatg atgcgccggt gacatcgcca ccacaccgcc tccgcgcaca tgatcgcgca
     3121 gcgccgttca tgtccgagcg cgcgcagccg ggcgagtccg ccatggaacg gtggggtcag
     3181 cgtgtagtcg gcatagttgt gaaagctttg gttttgccag aatgcattca cgtccggcgg
     3241 aacgtcccgc gtccgcgggc gcaagccgcc gagcgcggcg atgtgctcgt atccgatctg
     3301 gaattgggcc agcgtctcgg gaaggatatc gcaattgtat tgcggattgg tgcgcgagcg
     3361 cggaacgctg cggacatcga ccacgcaccc gatctgcgct ccctccagca actccacgaa
     3421 ctcccgcacc ggccgggttg aatgtccgat ggtgaagaac gggtgcgcca tctgtccctg
     3481 gcagtcgatt agcgacgcat gagccttagc gctgctccct tatgcagggc gacatggtcg
     3541 gtcttgtcgc ttttgatttc gtactgaggt tcgtccttgc tcgcgtggtg gacatatcct
     3601 ttgtaattga cgtcctttgt atgcaccttg acgattctcc cactgaccct cccggcctcc
     3661 gagttccagc ttacatggtc atgtagcttg aatcgttttt tcatacatca acgtacgccg
     3721 aaaaaggggg cggcctccga tcgaagaccg cccgatctca gttggtcgct ccaatcagac
     3781 cgcgaggatc gcaacgatct tgtagccacg atcgacgata atgatctcgt cgccgaccac
     3841 gaagtaccag aatctccgcc actccggatg gatgtccacg atgaccgacg ggacttcaac
     3901 cacgcgcacg ctcgtcggca ccacggtgcc cacgttgatc gagaagttga cattggtggc
     3961 gcgcggtaca ttcccgccgg ccaagaccgt ttgtctgatc ttggtacgtt gctccgtcgt
     4021 cagggttgcg ctgctggccg cctggccttg ctgagcttga ccctgctggg cctgcccctg
     4081 ctgcccggtc tggccttgtt gaccctgctg gcgttgcggg gcctgaccct gggtctgctc
     4141 tcgttgttgg cgctgcggct gcgcctgctg gtcttgctgc cgctggctct ggggcgcttg
     4201 gcccgtggtc tcccctttct gatcgcgctg cgattggccc tgcttctgtc cttgcatgtg
     4261 ttgctgcgat tgcccttgca tgtcgtgccc gcgctgggtc tcgctgcccg gagcggattg
     4321 tgattgttgg gctgcaccgc cgcgttgctc tcctccgcct ggcatgtttt gggctgacgc
     4381 caaggccact cccgccaata gcgcagccgt actgatcaaa agcctgttcc tcattggaac
     4441 ggttcctctc gttgttcccc gcgcgccgaa cgcttggccc ggggtaaaac gaagaagctt
     4501 tcatgtggtt ccccacgcga tatgggagtg accgcataag cacggcccgg ggaactttca
     4561 cgcgggcttt aatggagttt gctttgctct cgcgcgaggt tgagcgcacc cgacaacgct
     4621 ttaggctggc acgcggggaa gcgcgcacgt ggacccgcgc tgccgattgc tgacgctgtc
     4681 ggcacagcgt ggcgtggggg atgcgatctg gtcggcggtc gtaactggcg gagccgacga
     4741 acggctggct ttacacggct acaccgctgc cacgctcgcg gccttcttcc gtcctcgacg
     4801 ctccggaatg gggagatccc gcgcgcttgc aaacgtgcgc accacgagcc gctcgactcg
     4861 gccgcggatt tcgatctcgt ggcggggggc atcgccgagg tcgacgccag cgcgcaccgc
     4921 cacggcttcg gagatcacga gctcgcaggc gtaggtcttg gtgagggctt cgatgcggct
     4981 cgccgtgttg acggagtcgc cgacggcggt gatcgagact gcggtgccgt aacccatttc
     5041 gcccacgatg gcggggccgg tatggatgcc gatgccgatc cgcagcggct cgggtatgtc
     5101 gtgaatcaac gcgcgattga gggtctccat gcgctcggcc atgtccttgg ccgcgacgag
     5161 cgcctcgcgg cagcccgcct cgacgccgct gtcgaggcca aacagcgcca tcaccccatc
     5221 gccgatgaac ttgtcgatgc gcccgcctgc tgtctccacc gcatggccgg tctctgcaaa
     5281 gtatcgattg aggaggaata ccaagtcgta aggcagcttc ttttcggaga gctgggtgaa
     5341 ggacctgaga tcgccgaaca ggatcacgat ctcgcgctcg gcgccgtgga gatagccagg
     5401 acggctgaat ccgtcccggg cctgggcgct cgctggcagc agcggcgtca cgcgcagatc
     5461 gccgtgcggg cgcagttgac aggcgagccg cacgtcgggc gctgcgccca cgcggtgcaa
     5521 taccttcacc tcttcggccg cgggcggcac gatcgcctcc tttgctccgt cgaccctgat
     5581 gcggcaggtg gagcagcgcc cccgcccgcc gcacacggag gcatggggaa tgccagccat
     5641 ccggctcgcc tccaagaccg acacgccgcg cacgatctgc accacgcggc cgttcggata
     5701 ggtcagccgg acgatgccgc gccggcgctg ccagtaccaa cgcgccatcc gcgcggccag
     5761 aacggcaagg agcgccgcca cgaaggcgta acgcacccac accaccagct gatcgatgat
     5821 cgcggcgtcg gccggtgctg cgggcgcatg gtcctgcgcc tgctgggcga cccaggccgg
     5881 atcctcggcc atcgc
//
LOCUS       DATLJT010001122         4801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2376424,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001122 DATLJT010000000
VERSION     DATLJT010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376424"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..888
                     /locus_tag="VM910_25890"
     CDS             280..888
                     /locus_tag="VM910_25890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI65989.1"
                     /translation="MTEYERWEARYATPDYAFGKDPNYFLRSCKSLLPPSGRALAVAD
                     GEGRNGVWLAEQGLDVVSIDFSPSAQRKAKVLAAERHVKVAFELVDVHNWNYPEAEFD
                     VVVEIFTQFSSPAERALKWSGMKRSLKSGGLLIIQGYTPKQLEYGTGGPKQIENLYTR
                     AILERAFPEFRVLSIVEEECEIHEGTSHGGMSAVINLTATKP"
     gene            897..1307
                     /locus_tag="VM910_25895"
     CDS             897..1307
                     /locus_tag="VM910_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVI65990.1"
                     /translation="MVVHKGVGHYGTPITLDQAKKVMAAAEAKAKQNKWSVAVSIVDS
                     GGHANGTQLASIRIAEGKARTAVEFRRPTKALEDVIAAGGAGLRYFTVPDVNLMEGGV
                     PIVVDGKVVGGIGVSGVHSKDDAKIAQAGADAIK"
     gene            complement(1625..3442)
                     /locus_tag="VM910_25900"
     CDS             complement(1625..3442)
                     /locus_tag="VM910_25900"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI65991.1"
                     /translation="MGVKRRELLINAGSMAAILAGGELTSADMASAHRSHLPTRWQQE
                     ADIVVIGSGATGLPAAIVARESGASVIVIEAENHIGGHAITSGGNISLGGGTSVQKRY
                     GINDSPDLLFRDLTDWSVVEPNGFPDYRYNDREIVRAFADNSARTFEWLIAQGVVFVD
                     KAPESGGISVGNSVPRETHSAVLDWAMVQTGRPADPMLRGVLSSGEGLMRPLEAAARK
                     AGVQILLEHKMTAILREAPPSGRTLGVAADNKGQAVNIRARKAVIIATGGSTGNVNFR
                     RMFDPRLTEEYCGLAGMPWSNQDASGELAAMAIGASLWGLYNQTGEFGLSITKPGTIG
                     SQFGYAYLRWMPGSPVFDRVRATGLRVADWQDLILVNMLGKRFYDETGGQFTANNHNS
                     IEAYVQGSYRNTKNTKYNPNNFINAALAGIGDGHNGGGPIWAIFDADAVARERWDPNP
                     PNVDIKAGFFFTADALSELAKKIKMQYQRVPMPPGNLEQTVARYNSFVDGGVDQDFGK
                     PRPLHKIAKPPFYAAWATPVVHDTRAGLRINAKCQVIDVNGKVIEGLYCGGESAGGFS
                     QHGLGRAVCQGYIAGRNAALDVLINPGAVSVPPSVSNKP"
     gene            3588..4553
                     /locus_tag="VM910_25905"
     CDS             3588..4553
                     /locus_tag="VM910_25905"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVI65992.1"
                     /translation="MKRGDKVLGTLAAFLFLIGAAEPSAQTATPKRVNRAIELLEQGQ
                     PIYYTGSHAGTAGSFEQGKADAQTYADYISYDMEHPPFDVKGLADYMRGLVAGGPTRS
                     GHRMPPVIVNVPVNGIDEAAVHANAWMFAQVLATGVHGIMLTHADTPGAIRAFVEAVR
                     FPIHKQGLDKGLNEGRRGVHGAFIAAPIWGISPDEYLDRADPWPLNPNGELLLGVKLE
                     DRYALVNADENLKVPGIAIAEWGPADMALSLGVRGSGDLTRDPRMIEARGKVFAASRA
                     NRIFFLDSMNKSNVIDLINAGVMLGPASQETAQIGRKYTGRKMPW"
BASE COUNT         1015 a         1397 c         1419 g          970 t
ORIGIN      
        1 gcgcgcagca acccgacagc gcgcgcggtt ttctctgagg gcgacgcggc ccgcccaata
       61 acggcagcca acctccggtg cgcgcggatt agctagcatt accggtcaag gtaatgaggc
      121 cgcactcctt tagagcgtgt actcctagat cgcgattgtc cggcaatcgt tggctgattt
      181 tcgcgtccga cctgacctcg aggcgagctc gcaataggga caccgactat gtggggcact
      241 aagcacaact gggaagccag tactctcctg gagattttcg tgaccgaata cgagcgctgg
      301 gaagcccgct atgcaacccc cgattatgct ttcggcaagg acccaaatta ttttttgcga
      361 tcctgcaagt cattgttacc gccatccggt agagcattgg ccgtcgcaga tggcgaagga
      421 cgaaacgggg tctggctggc tgagcagggt ctcgacgttg tctcgatcga tttttcgcct
      481 tcagctcaac gaaaagcaaa agttttggca gccgaacggc acgtcaaggt cgcctttgag
      541 ttggttgacg tgcataactg gaattatccc gaagctgaat tcgatgtggt ggtcgaaatc
      601 ttcacacagt tcagttctcc ggccgagaga gccctgaagt ggagcggtat gaaacggtcc
      661 ctcaagtccg gcggcctcct gatcattcaa ggctacacgc caaagcaact ggaatacggc
      721 acgggtgggc cgaaacagat tgaaaatctt tatacgcggg caatcctcga acgagccttt
      781 cctgaatttc gtgtactatc gatcgtggag gaggagtgcg agattcatga gggaacgtca
      841 cacggtggga tgtccgccgt cattaatctg accgcgacaa agccatagga gttacgatgg
      901 tggtccacaa aggcgttggt cactatggca cgccaattac gctcgaccaa gccaagaaag
      961 tgatggctgc tgccgaagcg aaggctaaac aaaacaaatg gagtgtcgcc gtctcaattg
     1021 tcgatagtgg tggccacgcg aacggcacgc agcttgcctc gattcgaatt gccgaaggca
     1081 aggcccgcac cgcggtcgag tttcgtcggc cgaccaaggc gcttgaagat gtcattgcgg
     1141 ctggcggcgc ggggctgcgg tatttcaccg tgccggacgt caacctgatg gaaggcggcg
     1201 tcccgatcgt ggtcgatggc aaggttgtcg gcggcattgg tgtgtccggt gtgcattcga
     1261 aggacgatgc caaaatcgcg caggcggggg ctgacgccat caaatgacgg cttgcggcgt
     1321 catgcggctt cattcgaagt tgaggagcta ccagctttac gggggttcgg ccccggggtc
     1381 ttctcaagcc gaccgaccta cctccgaaca aacgattgcc gcagcatccg cgcgtggccg
     1441 ccgagcgcgc cgcggcacgc aagcggtggc ggttgcggca aacgaaccgc gcgcgcgagg
     1501 ccgcgacgtt ggcggcggag cgcgatgctg ccgcgggcgg tgacgtcgtc gcatagagag
     1561 ccttgcgggt gtcaacatca atgcgttaca gcactgagaa agcaacggag acaacaagat
     1621 gtactcaagg cttgttggaa acagaaggcg gcacagacac agccccaggg ttgatgagca
     1681 catccagcgc cgcgttccgt ccggcgatgt acccctggca aacggcacgg ccgaggccat
     1741 gctgtgaaaa gccaccagcg gactcgccgc cgcaatatag cccttcaatg accttgccgt
     1801 tcacgtcgat gacctggcac ttggcgttga tgcgcaagcc tgcgcgcgtg tcgtggacga
     1861 cgggcgtggc ccatgcggcg tagaacgggg gcttcgcgat cttgtgcagc ggccttggct
     1921 tgccgaaatc ctggtctact cccccatcaa cgaacgaatt gtagcgcgct accgtctgct
     1981 cgagattgcc aggcggcata ggaacgcgct ggtattgcat tttgatcttc ttggcgagtt
     2041 cgctgagcgc atcggcggtg aagaagaatc cggctttgat atcgacgttc ggtgggttcg
     2101 gatcccacct ctcgcgcgct acagcatcgg cgtcgaagat ggcccagatc ggcccgcccc
     2161 cgttgtgacc gtcgccgatg ccggcaagag ccgcgttgat gaaattattg ggattgtact
     2221 tagtgttctt ggtgttgcga tagcttcctt ggacgtaggc ctcgatggag ttgtggttgt
     2281 tcgccgtaaa ctggccacca gtttcgtcgt agaagcgctt gcccaacatg ttgacaagga
     2341 tcaggtcctg ccaatcggcg acccgcaaac cggtggcgcg cacgcgatcg aacaccgggc
     2401 tgcccggcat ccagcgcaga taggcatagc cgaattggct tccgatcgtg ccgggcttgg
     2461 tgatgctcaa tccgaattcg ccggtttggt tgtagaggcc ccagagcgat gcgccgatcg
     2521 ccatggcagc gagttccccg ctcgcatcct ggttcgacca tggcatcccg gctaatccgc
     2581 agtattcctc agtgaggcgc ggatcgaaca tgcggcggaa gttgacattg cccgtcgagc
     2641 cgcccgtcgc gatgatgacc gccttgcgcg cgcgaatgtt gaccgcttgt cccttgttgt
     2701 ccgcggcgac gccaagtgtg cggccagatg ggggcgcctc tcgcaagatc gcggtcatct
     2761 tatgctcgag caggatttgc acgccagcct tcctggccgc ggcctcgagg ggacgcatca
     2821 ggccctctcc cgacgacaga accccgcgca gcatcggatc cgcaggccta cctgtctgca
     2881 ccattgccca gtccagcacc gcgctgtgcg tttcccgcgg caccgagttg ccgaccgata
     2941 tcccaccgga ctcgggcgcc ttatcaacga aaacgacccc ctgagcgatg agccattcga
     3001 aggtccgggc gctgttgtcg gcgaaggcgc gaacgatttc acggtcattg taccggtagt
     3061 cgggaaaacc attcggctca acaaccgacc agtcggtcag gtcgcggaac agaaggtcgg
     3121 gagaatcgtt gatgccatat cgcttttgga cgcttgttcc accacccagg ctgatgttgc
     3181 caccgctggt gatggcgtgg cccccaatat ggttctccgc ctcgatgacg atgacggagg
     3241 ctccgctttc ccttgcaaca atcgcagccg gaagcccggt tgcgcccgaa ccaatgacga
     3301 cgatatccgc ctcctgctgc cacctggttg gcagatgcga tcgatgtgcg gacgccatat
     3361 cagcggatgt cagctcgcct ccggccaata tcgcagccat gctgccggcg ttaatcagca
     3421 actctcgcct cttcacgccc attggaacct cccatccata cctcgcgtcg tcatgtcgag
     3481 tcgctgcgat atgataatcg tcctgcttct gctaaacgga ggctgcaaaa atcatgtgat
     3541 accaattcag caaatccagc taggccatct ttagtccgga gacggcgatg aagcgcgggg
     3601 acaaagtact cggcaccttg gcagctttct tatttcttat tggggccgcc gaaccttccg
     3661 cacaaaccgc cacgccaaag cgtgttaatc gcgcaatcga gctcctcgag cagggccagc
     3721 cgatttacta tactggctca cacgccggga ctgcaggtag cttcgagcaa ggcaaggcag
     3781 acgcgcaaac ctacgccgat tatatcagct atgacatgga gcatccgcca ttcgacgtca
     3841 aagggctcgc tgactacatg cgtggtttgg tcgccggtgg gccaacaaga agcggacatc
     3901 gcatgccgcc agtcattgtc aacgttcctg tcaacggcat tgatgaggca gcggtacatg
     3961 cgaacgcctg gatgttcgcc caggtcctcg cgactggcgt tcacggcatc atgctgacgc
     4021 acgctgacac ccccggtgcg attcgggcat tcgtcgaagc cgtgcggttc ccaatccaca
     4081 agcaaggtct cgacaaaggg ctcaacgaag gtcgccgagg cgtacacggc gcgtttatcg
     4141 ccgccccgat ctggggaatt tcgccagacg aatacctcga cagagccgac ccatggccgc
     4201 ttaatcccaa cggcgagctg ttgctcggcg taaaactgga agatagatac gcattagtaa
     4261 atgcggatga aaacctgaaa gtgccaggca tcgcgatcgc tgaatgggga cccgccgaca
     4321 tggcattgtc tctcggtgtt cgcggttcag gcgacctaac gcgtgacccg agaatgattg
     4381 aagctcgagg aaaggtattc gccgcaagtc gagcaaatag aatattcttt ttggactcga
     4441 tgaataaaag caatgtcatt gatttaatca acgctggcgt gatgcttggt ccagctagcc
     4501 aggaaaccgc acagatcggt cgcaaataca ccggcagaaa aatgccgtgg taaacagcac
     4561 tacctcttaa cgctgatgtt tttaacgcga tcccccacca cggcccccga gacgttctcg
     4621 cggatcgcta cgcccggtcc gatggggcac gcctcagcta cggcaggaga ctgctgcgct
     4681 gcctcgccta tgacattcgt ttcagctgcg ggaaaccttt gtagcgatgg ctgtgatcgc
     4741 gatcgtggtt cgagaatccc ggtttcgtga gaaagccacg cggttcgatg aagctgtaga
     4801 t
//
LOCUS       DATLJT010001123         1843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_79098,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001123 DATLJT010000000
VERSION     DATLJT010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79098"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VM910_25910"
     CDS             <1..447
                     /locus_tag="VM910_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HVI65993.1"
                     /translation="DVAIRNEDLKIETMRAQGAGGQHVNKTESAIRITHIPTGIVVSM
                     QDSRSQHKNRASAMNILRSRIYDAERQRVEAARSADRKEKVGSGDRSERIRTYNFPQG
                     RVTDHRINLTLYKLPQVMSGEALGELIDALTTEHQAAQLAAQGAAA"
     gene            444..1319
                     /gene="prmC"
                     /locus_tag="VM910_25915"
     CDS             444..1319
                     /gene="prmC"
                     /locus_tag="VM910_25915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVI65994.1"
                     /translation="MNTFAGQSVETARRALAVRLKTSCIESAELEARTLVGAALGLDL
                     TGLVSSATRRLSTAEADRLDDFTRRRLAGEPVARILGKKEFWGVSFKLSKATLVPRPD
                     TETVVEQALEILRASPISAVRIADIGTGSGAILLALLLELPHAFGIGTDLSEGALGIA
                     QENAGQLGLGGRSGFVACNYAAALSGGFDLIVSNPPYVRSADIAGLDIEVRVHDPLLA
                     LDGGTDGLEAYRTLIPEAAGLLKPGGALVVEAGQGQAGDIGALMAAAGLMPPWAPKTD
                     LAGIPRAVTGRKMLV"
BASE COUNT          386 a          560 c          590 g          307 t
ORIGIN      
        1 gatgtcgcga tcaggaacga agacttgaag atcgagacga tgcgcgcaca aggcgccggc
       61 ggccagcatg tcaacaagac ggaatcggcg atccgtatca cccatatccc gaccgggatc
      121 gtggtcagca tgcaggacag ccgctcgcag cacaaaaacc gcgcgtccgc catgaatata
      181 ctgcgctcgc gcatctacga cgccgagcgc cagcgcgtgg aagcggcgcg ctcggccgac
      241 cgcaaggaaa aggtcggctc cggcgaccgc agcgagcgca tccgcaccta taatttcccg
      301 caaggccgag tcaccgacca ccgcatcaac ctgacgctct acaaactgcc gcaggtgatg
      361 tccggcgaag ccctcggcga actgatcgat gcgctcacga ccgagcatca ggcggcgcag
      421 ctcgctgcgc agggcgcggc ggcgtgaaca cattcgcggg gcaatccgtc gaaactgcgc
      481 gtcgcgcgct cgcggtacgg ctcaaaacgt cctgcatcga atcggcagag ctcgaggcgc
      541 ggacgcttgt gggcgcggcg ctgggccttg acctcaccgg tctggtttcg tccgcgacac
      601 gacggctcag tacggccgag gccgatcgac tggacgattt cacgcggcgg cgccttgccg
      661 gcgaaccggt cgcgcgcatt ctcggcaaaa aggaattttg gggagtttcg ttcaaactgt
      721 cgaaagcgac gctggtgccg cggccggaca ccgaaaccgt ggtcgaacag gccctggaaa
      781 tcctgcgcgc gagccccata tcggcggtgc ggatcgccga tatcggcacc ggctcgggtg
      841 caattctgtt ggcactgctg ctggaactgc cgcacgcgtt cggcatcggc accgatctca
      901 gcgaaggcgc tcttggcatc gcgcaggaaa acgccggcca actcggactt ggcggacgtt
      961 caggcttcgt agcgtgcaac tatgccgcgg ctttgtcggg cggttttgac ctcatcgtct
     1021 cgaacccgcc ctatgtccgc tctgcggaca tcgcaggcct tgatattgag gttcgcgtgc
     1081 atgacccgct tctcgcgctc gatggcggaa ccgacgggct tgaggcgtac cgtaccctga
     1141 tccccgaggc cgcaggcctc ttgaagcccg gaggggcctt ggtggtggag gccggccaag
     1201 gccaggctgg cgacattgga gccttgatgg cggcggcggg cttgatgccc ccatgggccc
     1261 cgaaaaccga cctggcgggt attccccggg ctgtaacagg ccgaaaaatg ctggtttgaa
     1321 acccctgtgg aacgcaaaaa cctcttggaa tattccttgg gaccgactac gtttcggcca
     1381 caacaccggc gcaggtcccg agacgaagcg ggccaaatcc agggttctca agccagagcc
     1441 acgccgggtg aaaggttccg aaacgcgggt cgaattgagc gcgagggctt aagtcaagct
     1501 gcgccgcttg cgaccgcaaa tcgaacgaaa gcctgatttt gcgcttgaag acctactcaa
     1561 gaaaacaggg tttgttttga caggctgaac gaagggttcg ccggggcgcc ggcgggtggg
     1621 gaacgcgtct ttgttgaaga cgacggttga cgaaatcggc agggatcgag acggcagcgg
     1681 gtttccgtgc gcttgcaccc gtgcgcttgc cagtttcggt cgcgccggca attcagtgct
     1741 ttaactcagg gctggaaata aaggcaggac atgagaaacg gtcaaaataa caagcggatg
     1801 cgcaaccgga acaataacaa caacaataac aaccggcgcg gcc
//
LOCUS       DATLJT010001124         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2608402,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001124 DATLJT010000000
VERSION     DATLJT010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608402"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1245
                     /locus_tag="VM910_25920"
     CDS             181..1245
                     /locus_tag="VM910_25920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI65995.1"
                     /translation="MKFGINFFPAFRPNQKTTAEYYAQCLRVSERADELGFSSIKTVE
                     HYFHDYGGHSPNPIVLLAAIAARTRCVRPITGAVIPAFHHPIKLAGELAMLDNISYGR
                     LDVGFGRAFIPEEYAAYGLDMQDSRAMFDEGIAAIKRLWTEERVTHEGRFWNFKDVHS
                     MPRPFQKPHPPIWVAAVASEESFVNAAKNGYHIMIVPYAGGLERTAALVKTYRQVWRE
                     SGHAPGTEQVQMSFHAYVSHTRNEAVQGFIPAMRTYLDVFTEAVSSWEGLKSGQYVGY
                     ERLVQSISAQTPEGNIEKGYAFVGTPDEVITQVRHLRELFGEHEPSLQVTFGGVTDDE
                     ALRTVDLFAQHIMPAFARAA"
     gene            1565..2206
                     /locus_tag="VM910_25925"
     CDS             1565..2206
                     /locus_tag="VM910_25925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65996.1"
                     /translation="MKRRLFISLLGGTAAWPLTARAQQTVGKFPKIGFLQGSQNENVA
                     AFIQALQAAGYTDGQNVRIETRIYGTMLDRLGEFASELVDLQCKVILAAAPYAIRAAA
                     GATAPASPSVAQINMSATPSAGRGRALKPRPADTRHRLVPNYRNPNSIDLPPGVTLSR
                     KDAKSRTGGRKLVMLMARPIPRADLKKQESRWACRRSGSSHVSVGLTGLLNPT"
BASE COUNT          482 a          693 c          700 g          422 t
ORIGIN      
        1 ctaggtaatc ctgttggatg gcgacttgtt ttccgggccg ttcatcgccg gcatgatcag
       61 gcgcgccgaa aggctgccgc ggcggagccc cccttgcccg ggcgccagtc gaaagctgat
      121 tgtgtccgct cgacccatac agggcttgat gacaggctac gaccgcggga gcattgagaa
      181 atgaaattcg gaatcaattt ctttccggca ttccggccga accagaagac gacggccgaa
      241 tactacgcgc aatgcctacg cgtgagcgag cgagccgatg agctcgggtt tagctcaatc
      301 aaaacggtgg aacattactt ccatgattac ggaggccatt cgcccaatcc gatcgtcctc
      361 ctggccgcca tagcggcccg caccaggtgc gtgcgcccga tcaccggcgc ggtgatcccg
      421 gcctttcacc atccgatcaa gctagcgggc gaactcgcca tgctggacaa catctcgtat
      481 ggccgcctcg atgtgggctt tggccgggct ttcatccctg aggagtatgc ggcgtatggg
      541 ctcgacatgc aggacagccg agcgatgttc gacgaaggca tcgccgcgat caagcggcta
      601 tggaccgagg agcgcgtgac gcatgagggc cgattctgga acttcaagga cgtgcattcc
      661 atgccgaggc catttcagaa gccgcatccg cccatttggg tcgcagcggt cgcctccgag
      721 gaatcgttcg tgaacgccgc caagaacggc tatcacatca tgatcgtgcc ttatgcgggc
      781 ggtctggagc gcacggccgc tctggtgaag acgtaccgac aggtctggcg agaatccggc
      841 catgctccgg gtacggagca ggtccagatg tcattccacg cctatgtgtc gcacacccgt
      901 aacgaagcgg tgcagggctt catcccggcc atgcgtacgt atctcgacgt gttcacggag
      961 gccgtcagca gctgggaagg actgaaatcg ggccagtacg tcggctacga gcggctggtg
     1021 cagtcaatca gcgcgcaaac tcccgaagga aacatcgaaa agggctatgc cttcgtcggc
     1081 acgcccgacg aggtcatcac gcaggtgcga catttgcgtg aattgtttgg cgagcacgaa
     1141 ccgtcattgc aggttacctt tggtggcgtg acagatgatg aggcattgcg gacagtggac
     1201 ctgtttgccc agcatatcat gccggcgttt gcgcgtgcgg cgtaagcgac gcaggacacc
     1261 tcgagtatct tcactcggtt actaccacct caagccgcgc cgcggtggcg ggaccgcaat
     1321 tccgcattgc agtggtctcc tggcggcgcc gtgatgtgct atcgtttggg tggcagccac
     1381 gacaccgacg gcctttccac ccgaaaccat gcaaaagagc ctgctgttca tccctgaacc
     1441 cgtggccgta cccgcctgtg cagcacgtca caaaaatcga gctgatcatc aacctcaaga
     1501 ccgcgagagc gctcagtctc gacgtgccga cctcactgct attgcgcgcc gacgaggtga
     1561 ttgaatgaag aggcggctgt tcatctcgtt gctcggcggc acagcagcgt ggccgctcac
     1621 ggcgcgcgcg cagcagacgg tgggcaagtt cccgaaaatc ggctttctcc agggctcgca
     1681 aaacgagaac gtggctgcat tcattcaagc tctccaagcc gctggataca cggacgggca
     1741 gaacgtacgg attgagacac gcatatatgg aacgatgctg gaccggctcg gcgaatttgc
     1801 aagcgaacta gttgacctcc agtgcaaagt catccttgcc gctgccccat acgcgatccg
     1861 ggccgctgca ggagccacgg cgcctgcaag cccatctgtc gcgcagataa acatgtcggc
     1921 aacgccgagc gcaggtcggg gtcgggcact gaaaccacgg ccggcagaca cccggcatcg
     1981 gctggtccca aactatcgaa atccgaactc catcgacctg ccgccggggg tgaccttgtc
     2041 tcgcaaggac gcaaaaagcc gaacgggcgg tcgcaagctg gtgatgttga tggcgcggcc
     2101 gattccgcgt gcagatctga aaaagcaaga gagccgctgg gcgtgtcggc gcagcggctc
     2161 gtctcacgtc agtgttgggt tgactggcct gctcaaccct acctgaagcc agcgcagact
     2221 atctagaccc tctaaccatc gtcgcaggag ccgatggacc gaatggcgcg ccagcgacgt
     2281 taagtggaag gacgaca
//
LOCUS       DATLJT010001125         4436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1918167,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001125 DATLJT010000000
VERSION     DATLJT010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918167"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..420)
                     /locus_tag="VM910_25930"
     CDS             complement(163..420)
                     /locus_tag="VM910_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65997.1"
                     /translation="MVLGHFPKRAKGGEAKVILDSLTMAGIDPDRVVEITVGQHSPLH
                     GALPSWHFKAHSAGKLPFQLVESRQLARRGRRIDVANKFGH"
     gene            complement(487..1773)
                     /locus_tag="VM910_25935"
     CDS             complement(487..1773)
                     /locus_tag="VM910_25935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HVI65998.1"
                     /translation="MAGLRAVCAAFPDSRKGRRGNIAIADFGLSAFALFFMQSASFLA
                     FQRALEKGQGRSNCETLFGIGRIPSDNYIRDMLDGADPALLQPCFERMEQLVAVPPLR
                     AAFVRLDDRVPIAWDGTEYFCSQKLGCPHCLRRKRANGAVESYHILLAVTAVAPGHSK
                     VIPLFPEFIAPQDGAEKQDCERNAAKRWHAKHADRLRPLRPVYLGDDLFACQPIVSML
                     TQAGDDFIFTCKQTSHKALYDFIDGAEPERHVETIRKGKAVETRRYRWIEGVPLRDGR
                     DAALVNWVACEIIDRHGAVKYSVAWVTSLPVGKASVADIAACGRSRWKIENETFNVMK
                     NHGYELEHNFGHGEKFLAMTLAALNLLAFAWHSALDIVDPPWQTARQVAAKRASFFAH
                     LLTLTSYVVFPSWSALLQALTTFTIPPELLQTQKIE"
     gene            complement(1858..2016)
                     /locus_tag="VM910_25940"
     CDS             complement(1858..2016)
                     /locus_tag="VM910_25940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI65999.1"
                     /translation="MGRALTVAMILISGRAFALTDSAPIGSRQPVFGLAAKQPALRIY
                     YGDGLQQF"
     gene            2262..3398
                     /locus_tag="VM910_25945"
     CDS             2262..3398
                     /locus_tag="VM910_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI66000.1"
                     /translation="MAAWRQALELAIGEEDMATLRVIARSRNEPARRVERARLLLAYR
                     EDPSFFAVAQAAGVHHQTVQRCVERALAYGPIAALDDLPRPGREPTITAEAKAWVVNL
                     ACRKAKELGYPHELWTTRLLARHAREHGAAEGHPCLGQLAQGTLCNILNEQEIKPHKV
                     RYYLERRDPEFKRKMAEVLCVYREVKLIKEGAAATGDGVAIISYDEKPGIQAIATTAP
                     DLPPEPGVHATFARDHEYERKGTVSLLAGIDLLTGRVHALVRDRHRSCEFIEFLQLVD
                     VAYPPHTAIKLILDNHSAHISKETKAWLANQPPRRFEFTFTPTHGSWLNLIEGFFSKL
                     ARSVLRHIRVTTKQELKDRILAAMDEFNRHPVVHTWSYKLGDVA"
     gene            3435..3688
                     /locus_tag="VM910_25950"
                     /pseudo
     CDS             3435..3688
                     /locus_tag="VM910_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460318.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3707..>4436)
                     /locus_tag="VM910_25955"
     CDS             complement(3707..>4436)
                     /locus_tag="VM910_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI66001.1"
                     /translation="TSTEDSGLFGHILPRFRAASGIDVHVVAVGTGQALTIGARGDAD
                     ALLVHDRAGEDKFVADGFGIDRRDVMYNDFVIVGPRSDPASIRGLRSAREAFALIAAA
                     RAAFASRGDDSGTNRMELRLWKSAGLAPNKTSAWYRDLGQGMGPTLNVAAGLNAYTLT
                     DRATWANFKNRQDLEILTEGDPALFNPYGSILVNPAKWPHVKAAEAKTWHEWLTSKAG
                     LDAITSYRIDGEQLFFPPRPTANR"
BASE COUNT          843 a         1316 c         1436 g          841 t
ORIGIN      
        1 attccggcca tgctcagcct cgatccgcag gccgttcgtc tcgacagcct gggttatctg
       61 atgcatcgga tcgggcgcga cggccggttg atgttggagt gaaaggaacc gagcgcggag
      121 ctaccgcagg gcaattgacc gcgtggggag gcgagcagcg tgtcagtgac cgaacttgtt
      181 ggcgacatcg atgcgcctgc cacggcgtgc gagctggcga gattcgacga gttggaacgg
      241 caatttgcca gcgctatgcg ccttgaaatg ccaactcggc agcgcaccat ggagcggaga
      301 atgctggccg actgtgattt caaccaccct gtccggatca atccccgcca tggtcaagct
      361 gtcgaggatc accttcgcct cgccgccttt ggcgcgtttt gggaaatgac cgagtaccat
      421 gggcgtcgcg gtcgcccata accgcgacca gtaattctaa aagtacgtgg tgcgcggttc
      481 gcggttctat tcgattttct gggtctggag tagttccggc gggatcgtga aggtggtcag
      541 ggcttggagg agggcggacc aggagggaaa gacgacatag ctggtcaagg tcaggagatg
      601 ggcgaagaag ctcgctcgct tcgccgcaac ttggcgcgcc gtctgccagg gcgggtcgac
      661 gatatcaagg gccgaatgcc aggcgaaggc gaggagattg agcgcggcga gcgtcatcgc
      721 caggaatttt tcgccgtgcc cgaaattgtg ttcgagctca taaccgtgat ttttcatcac
      781 gttgaaggtt tcgttctcga tcttccagcg tgaccggcca caggcggcga tgtcggcgac
      841 gctggctttg ccgaccggca ggctcgtcac ccaggcgacg gaatatttga cggcaccgtg
      901 gcggtcgata atctcgcaag cgacccagtt gaccagtgcg gcgtccctgc cgtcgcgcaa
      961 cggcacccct tcgatccacc gatagcggcg cgtctcgacc gccttgccct tgcggatggt
     1021 ttcgacgtgg cgttcaggct cggcgccgtc gatgaaatcg tagagcgcct tgtgtgaggt
     1081 ttgcttgcaa gtgaagatga agtcatcgcc ggcctgcgtc agcatgctga cgatgggctg
     1141 gcaggcgaac agatcgtcgc cgagatagac gggacgcagg gggcgaagtc tgtcggcgtg
     1201 cttggcgtgc cagcgcttgg cggcgttgcg ttcgcagtcc tgtttttcgg cgccgtcctg
     1261 cggcgcaatg aactcgggaa acagcgggat gaccttggaa tggccgggcg ccaccgccgt
     1321 cacagcgagc aggatatggt agctctccac tgcgccgttg gcgcgctttc gccgcaggca
     1381 gtgcgggcag ccgagctttt gcgagcagaa atactcggtg ccgtcccagg cgatcgggac
     1441 acggtcgtcg agcctcacaa aggccgcccg cagcggcggc acggcgacca attgctccat
     1501 gcgctcgaag caaggctgca acagcgccgg gtcggcgccg tcgagcatgt cacggatgta
     1561 attgtcggag gggattcttc cgatcccgaa cagggtctcg cagttcgagc gcccctggcc
     1621 tttctccaag gcgcgctgga acgccagaaa cgaggcgctt tgcatgaaaa acagggcaaa
     1681 cgccgaaagc ccgaagtccg ctatcgcgat gttgccgcgg cgtcccttgc gggaatccgg
     1741 aaaggcggcg cagaccgcac gcaatccagc caaaagggac gccaatagct ccaccccaat
     1801 ggtggaatca cttctccgct gatttgggaa tccctaaaat gcacgcctgg gctactttta
     1861 gaattgctgg aggccgtccc cgtagtagat gcggagagcg ggttgcttcg cggcgagccc
     1921 gaacacaggc tgtcgcgatc caatcggcgc ggagtcggtc agcgcgaacg cgcggcccga
     1981 aatcaggatc atcgcgacag tcagcgcacg acccatggtg gcaagcctcc gaaaaacatg
     2041 gggtgctgat aaagcgacgg acgcggatca atctccgtcc cgtcggaaac ccaagccatg
     2101 ttgttacgcc aataccagct ctcgctgcca agaacgctcg caacgccagc gacgacattg
     2161 actatttcat cgattctctg aaaccaatca tttgggtggg attcatttta cctgacatga
     2221 cttgtagcct tgctgtttga ttcgcaatga ggattgagat catggcggct tggcggcagg
     2281 cgctggaatt ggcaattggc gaggaggaca tggcgacgtt gcgcgtaatc gcgcggtcgc
     2341 gcaacgaacc ggcgaggcgg gttgagcggg cgcgcctgct gctcgcctat cgggaagacc
     2401 cgtccttttt tgcggtggcg caggcggctg gggtgcacca tcagaccgtt cagcgctgcg
     2461 ttgaacgggc gctggcctat ggtccgatcg cggcgctcga cgatctgccg cggcctggcc
     2521 gagagccgac aatcaccgcg gaggccaaag cctgggtagt gaacctcgct tgccgcaaag
     2581 ccaaggagct tggctatccg cacgagctgt ggacgacgcg ccttcttgcc cgccatgcgc
     2641 gcgagcatgg ggcggcggag gggcacccct gccttggtca actggcgcag ggcacgttgt
     2701 gcaacatcct caatgaacag gagatcaagc cgcacaaagt gcgctactac ctggagcgcc
     2761 gggacccgga attcaagcgc aagatggcgg aggttctgtg cgtctaccgt gaggtcaagc
     2821 tcatcaaaga gggcgcagcc gccaccggcg atggggtggc gatcatctcc tatgacgaga
     2881 agccgggcat tcaggcgatc gccaccacgg caccggactt gccgcccgag cccggcgtgc
     2941 acgcgacctt tgcgcgcgat catgaatacg agcgcaaagg caccgtcagt ctgctggcgg
     3001 gcattgatct gttgaccgga cgggttcacg ccctggtcag ggatcgccac cgcagttgcg
     3061 aattcatcga gtttctccag cttgtcgacg tcgcttatcc gccccacacg gcgatcaagc
     3121 tgatcctcga caatcattcg gcgcacattt ccaaagagac caaggcctgg cttgcgaacc
     3181 agccgcccag gcgcttcgag tttacgttca cgccgacgca cggctcctgg ctcaatctca
     3241 tcgagggttt cttctcgaaa ctcgcccgct cggtgttgcg ccatatccgt gtcaccacca
     3301 aacaagagct caaggatcgc atcttggccg ccatggatga attcaaccgt catcccgtcg
     3361 tccacacttg gtcctataag ctcggcgacg tcgcctgata tgattcgaac ttcaaaatcg
     3421 atgaactagt cagcggcgca tcccgccttc aggcgattcg gccattgatg agcatgggtc
     3481 gggagggatt gctgactgct tttctggcgg cctcgccatc gcggaagtgg gagccatcat
     3541 tatcgttggc ggtaacatcc gggaatacac gcgcaccatg acgacgacaa tcgccttgga
     3601 aacgagccgg ggtgacctga gcttggcgct ggcgctcggg atcatcctgc ttggcctttc
     3661 gatggcggtg agcgccgcga gcctctaagc gcggcctgcc tgaacgctag cggttggcgg
     3721 tagggcgcgg gggaaagaaa agctgctcgc cgtcgatgcg atacgacgtg atggcgtcga
     3781 ggccagcttt tgaggtcagc cactcgtgcc aggttttggc ttcggccgcc ttcacatgcg
     3841 gccacttggc ggggttgacc aggatgctgc cgtaggggtt gaacagggcc gggtcgcctt
     3901 cggtgaggat ttcgaggtcc tggcggttct tgaagttggc ccaggtggct cggtcggtga
     3961 gcgtgtaggc gttgagcccg gcggcgacgt tgagggtcgg ccccatgccc tgacccaagt
     4021 cgcgatacca ggcgctggtt ttattcggag cgaggcccgc cgatttccac agacgcagct
     4081 ccattcggtt cgtgccgctg tcatcgccgc ggctcgcaaa agcggcccga gcggcggcaa
     4141 tcagggcgaa tgcctctcga gcgctccgca gtccccgaat actggcagga tcggaccgcg
     4201 gcccaacgat gacgaaatcg ttgtacatca cgtcacggcg gtcgatgccg aacccgtcgg
     4261 ctacgaattt gtcctcgccg gcgcggtcat gcaccaggag cgcgtccgca tctccgcgcg
     4321 caccgattgt gagcgcctgg ccggtgccga ccgcgaccac gtgaacgtcg atgccgctgg
     4381 ccgctcgaaa gcgcggcagg atgtgaccga acaagccgga gtcctcggtg gaagtc
//
LOCUS       DATLJT010001126         6701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3756070,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001126 DATLJT010000000
VERSION     DATLJT010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756070"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VM910_25960"
     CDS             complement(<1..383)
                     /locus_tag="VM910_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HVI66002.1"
                     /translation="MVAVAARRFGAAQLEVIVADITTLSVDAIVNAANTSLLGGGGVD
                     GAIHRAAGPELLAECRKLGGCETGDAKITKGYRLPAKHVIHAVGPVWNGGAKGEDELL
                     ASCYRRAIELYRANELSSIAFPAIST"
     gene            complement(386..718)
                     /gene="grxD"
                     /locus_tag="VM910_25965"
     CDS             complement(386..718)
                     /gene="grxD"
                     /locus_tag="VM910_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HVI66003.1"
                     /translation="MSIEQFIDNEVKSNDVVLFMKGTPQFPQCGFSGQVVQILDHIGV
                     GYKGLNVLESAELRNGIKVYSNWPTIPQLYVKGEFVGGCDIIREMFQAGELQQLFADK
                     GIPVDAAA"
     gene            1018..2274
                     /gene="egtB"
                     /locus_tag="VM910_25970"
     CDS             1018..2274
                     /gene="egtB"
                     /locus_tag="VM910_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HVI66004.1"
                     /translation="MTKPASAAAAFSDSGTSLFSDPDTLSHNLVESFLAVRGETERRA
                     APLSAEDQVVQSMPDASPIKWHRAHTTWFFEQFLLGEHCKGYQPFHPDYAYLFNSYYV
                     SAGPRHARHQRGLLTRPGADDVTAYRRHVDAAVVKLFQISGEEMLRKLAPLVEVGLNH
                     EQQHQELMLTDMLHAFAQNPIPPAYDADWRFPSATRTSDAWVTLSEGIHTVGHADDSF
                     HFDNEKPAHRALVGPVKLNRNLVTNADWLAFMNDGGYSTATLWLMDGFATASSEGWQA
                     PGYWREIDGAWHVMTLAGLKPVDPSAPVCHVSYYEADAFARWAGKHLPTEMEWEVAAR
                     GNHLNDAFGIVWQWTRSAYAPYPGYRAVEGALGEYNGKFMVNQLVLRGSSLATPAGHS
                     RITYRNFFYPHHRWQFTGLRLADYTA"
     gene            2323..3291
                     /gene="egtD"
                     /locus_tag="VM910_25975"
     CDS             2323..3291
                     /gene="egtD"
                     /locus_tag="VM910_25975"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HVI66005.1"
                     /translation="MNVHASALADAHLPDERTSAFARDAIDDLSQHPKRLSPKYFYDA
                     AGSELFEAITKLPEYYPTRTELGILRDRSREIAALIPKGAALVEFGAGATTKVRLLLE
                     RSAFGAYVPVDISGDFIRAQAEALRKDFPSLSVYPLAADFTAPFALPAAVADMPKVGF
                     FPGSTIGNFEPHEACRFLRSARDILGPEAQMIVGADLEKDERVLYDAYNDAAGVTARF
                     NLNLLVRINRELGGNFDISAFTHRAIYNRERYRIEMHLISKKPQTVRLLGTSFSFRPG
                     ESIHTESSYKYSVDRFAALARSTGWKVRESWTDRAKMFSVHALLAA"
     gene            complement(3332..4513)
                     /locus_tag="VM910_25980"
     CDS             complement(3332..4513)
                     /locus_tag="VM910_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HVI66006.1"
                     /translation="MAVDNERGALFRAIVPNQHNRVTYAELFFDLVFVFAVTQVSHTL
                     LNRFTPLGAVQTTLLFLAVWWVWVYTTWITNWLNPELTPVRILLFLLMLAGLVLSTSI
                     PHAFETRGLWFASAYATMQVGRTAFWLASIPGERTAVRWNAIRILTWLSTSAVFWILG
                     GFAQDYWRLMLWAVALAIEYVGPVARFWIPKYGASAVEDWYVEGGHMAERCAGFIIIA
                     LGESIVVTGATFADLTWTHEIVAAFASAFVAAIAMWWIYFHRGAEAGSELISKASDSG
                     RLARSAYTYLHMPIVAGIILTAVADEVVLKHPSGSSDLKTALSAIGGPLLFLIGTILF
                     KHKIRGFLQLSHRVGIAALAVLAYFARDLSPLMLSVLTTALLVVVAIWESMSLRSRAR
                     E"
     gene            complement(4549..4785)
                     /locus_tag="VM910_25985"
     CDS             complement(4549..4785)
                     /locus_tag="VM910_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HVI66007.1"
                     /translation="MPMDARDIESMIKAAIPDAEVTIRDLAGDGDHYAATVISESFRG
                     KSRVQQHQIVYQSLRGQMGGVLHALALQTGVPEG"
     gene            5019..5378
                     /locus_tag="VM910_25990"
     CDS             5019..5378
                     /locus_tag="VM910_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HVI66008.1"
                     /translation="MKLPGPDHPISITQNPKRVRVIADGIVIADSTRALTLKEARYPA
                     VQYVPREDANMGLMQRTDRVTHCPYKGDASYFSIVANGKTLDNAIWTYETPYPAMAEI
                     SGHLAFYPDKVKIEEVA"
     gene            5581..>6701
                     /locus_tag="VM910_25995"
     CDS             5581..>6701
                     /locus_tag="VM910_25995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVI66009.1"
                     /translation="MMTSISQTAAPAQARAVPKAKSRNAALDRSRTFLTLVVLLHHSV
                     IPYTYFGHTDPKSWIGFDMIVLATDSFFMAMFFFLSGLFVWTGLARKGPWDYLRDRLI
                     RLGLPFVICAFTLIPLAYYAVALRQTPDLTFAAFWWKTITVGPWPSGPIWFLWVLFGF
                     DLVASLLYSLSPKLLDPINRLSLRGYEQPAAFFAVLLAVTASLYIPGRVHFGAGSWFE
                     FGPFSVQHGRVLLYATYFFFGAGLGAANFDRGLLGADGQLAKSSWGWAILALVPYCLM
                     WVLIYIKRELLGNPMQLPDWYEATYGLFFASFSVAIMFVILAYFLRFKQSGWSILDPM
                     QPEAYGMFLVHYPIVLWLQYWLYDFDISAIAKALIAFLLT"
BASE COUNT         1293 a         2152 c         1958 g         1298 t
ORIGIN      
        1 gtcgaaatcg cgggaaaggc gatcgaggag agttcgtttg cccggtagag ttcgatggca
       61 cggcgataac aggaggcaag cagctcgtcc tccccctttg cgccgccatt ccaaaccggt
      121 ccgaccgcgt ggatcacgtg ctttgccggc agccggtagc ctttggtgat cttggcgtcg
      181 ccggtctcgc agccgccgag cttgcggcat tcagcaagca gctcgggtcc cgccgcgcga
      241 tgaattgcgc catcgacccc gccgccaccc aggagcgacg tgttggctgc gttgacgatg
      301 gcgtcaacgc tcaaggttgt aatgtcagcg acgatgactt cgagctgcgc ggcgccaaaa
      361 cggcgcgctg caactgcgac caagctcagg ccgccgcgtc gaccgggatg cccttgtcgg
      421 caaagagctg ctgcaactcg ccggcctgga acatctcgcg gataatgtcg caaccgccga
      481 cgaactcgcc cttgacatag agctgcggaa tggtcggcca gttggaatac accttgatgc
      541 cgttgcgcag ttcggccgat tcgagaacgt tcagtccctt atagcccacg ccgatgtggt
      601 cgaggatttg cacgacctgt ccggagaagc cgcactgggg aaactgcggt gtccccttca
      661 tgaacaacac cacgtcgttc gacttcacct cgttgtcgat gaattgctcg atgctcatat
      721 ccatttcctt ttggggcgca gcgccctgcc accttcgtgg gcacgcctaa tccgtgtaac
      781 ccgatactgc cttcatatgt agcgcaaacc gttgtgtatc caaagcaaaa tggcgaagcc
      841 ctggcatgcc ggaaaggcct gcccgggcgc cacttctggg gcccgttcat agcctgacgc
      901 cgccaatatc atggccaggg gaggcttttt tcccgaaacg gagcccctat gtaggacagg
      961 ccgtgccttt ggaaccagtt tgcaagtcca acgttttctc acccgacgga ggggtcagtg
     1021 acaaaaccag catccgccgc ggccgcgttt tccgattccg ggacatcact tttttctgat
     1081 cccgacaccc tgtcccacaa cctggtggaa agcttccttg ccgttcgcgg cgagaccgaa
     1141 cgccgtgccg ctcccctgag cgccgaggac caggtcgtac aatcgatgcc ggacgcaagt
     1201 cccatcaaat ggcaccgcgc ccataccacg tggttcttcg agcagttttt gctgggcgaa
     1261 cattgcaagg gttatcaacc gtttcatccc gattacgcct atctgttcaa ttcctattac
     1321 gtgagcgccg ggccgcgtca cgcccgacac caacgcggtc tcttgacccg ccccggcgcc
     1381 gatgacgtca ccgcctatcg ccgccatgtc gatgctgcgg tcgtcaagct tttccagatt
     1441 tccggcgagg agatgctgag gaagctcgcg cctctcgtcg aagtcggcct caatcacgag
     1501 cagcagcatc aggaattgat gctgaccgac atgctgcatg cgtttgcgca aaatccgatt
     1561 ccgccggctt acgatgccga ctggcgcttc ccctccgcaa cccgcacaag cgacgcatgg
     1621 gttacgctca gcgaaggcat ccacaccgtc ggccacgcgg acgacagctt ccacttcgac
     1681 aatgagaagc cggcgcatcg cgcgcttgtc gggcccgtca agctcaaccg caacctcgtc
     1741 accaatgccg attggctcgc tttcatgaac gacggcggct actcgaccgc cacgctctgg
     1801 ctgatggatg gctttgcgac cgcaagcagc gagggatggc aggcgcccgg ctattggcgc
     1861 gagatcgacg gcgcctggca cgtcatgacg ctggcgggcc tcaagccggt cgatccgtca
     1921 gctcccgtct gccacgtcag ctattacgag gcggatgcct tcgcacgctg ggcgggcaag
     1981 catttaccga ctgaaatgga gtgggaggtc gccgcccgcg gcaatcatct caatgacgcc
     2041 ttcggcatcg tctggcagtg gacccgcagc gcgtacgcgc cctatcccgg ctatcgcgcg
     2101 gtcgaaggag cgctcggcga atacaacggc aagttcatgg tcaatcagtt ggtgctgcgc
     2161 ggctcctcgc tcgcgacccc ggcgggacac agccgcatca cctatcgcaa cttcttctat
     2221 cctcaccacc gctggcaatt cactggacta cggctcgccg actacaccgc ttaacccatc
     2281 tcgcgataaa agcgcgccgg aacgcgcgtt caggagagta tcatgaatgt gcacgcttcc
     2341 gctttggctg acgcgcatct tcccgacgaa cggacatcag ctttcgcgcg tgacgccatc
     2401 gacgatctgt cgcaacatcc caagcgcctg tcgccgaaat atttctacga tgcagccggc
     2461 tcggaattgt tcgaggcgat cacgaaactg cccgaatatt atccgacccg caccgagctt
     2521 ggcatcctgc gcgaccgcag tcgcgagatt gcagcgctga tccccaaagg cgctgcgctc
     2581 gtcgaattcg gggcgggcgc cacgacgaaa gtccgcctgc tgctcgaacg atcggcgttc
     2641 ggcgcctacg ttcccgtcga catctccggc gatttcatca gggcgcaggc cgaggccctg
     2701 cgtaaggatt ttccatccct ttcggtctat ccgctggcgg cggattttac ggcgccattc
     2761 gcgttgccgg cagcggtcgc cgacatgccc aaggtcggtt tcttcccggg ctcgacgatc
     2821 ggcaatttcg agccgcacga ggcctgccgc ttcctgcgca gcgcgcgcga catcctgggg
     2881 ccggaagcgc agatgatcgt cggcgccgat ctcgagaagg acgagcgcgt gctttatgac
     2941 gcctacaacg acgcggccgg cgtcaccgca cgcttcaacc tgaatttgct ggtgcgcatc
     3001 aaccgcgaac tcggcggcaa tttcgacatc tccgccttca cccatcgcgc gatctataac
     3061 cgcgagcggt atcgcatcga gatgcacctg atcagcaaaa agccgcaaac cgtgcgcctg
     3121 ctggggacga gcttctcgtt ccgcccgggc gaaagcatcc acacagagag cagctacaaa
     3181 tacagcgttg accgttttgc ggctctggcg cgcagcacag gctggaaggt ccgggagtcc
     3241 tggaccgaca gagcgaagat gttctcggtc cacgcgctgc tcgcggcgtg acaactccgc
     3301 ggcggcggaa gttgcggata cgaaatcgcg gctactcgcg cgccctggag cgcagcgaca
     3361 tcgattccca gatcgcgacg acgaccagga gcgccgtggt caaaaccgac agcatcagtg
     3421 gcgacaggtc gcgcgcgaaa taggccagca ccgcgagcgc ggcgatgccg acgcgatggg
     3481 agagttgcag gaagccgcgg attttgtgct tgaacaggat ggtgccgatc aggaacagca
     3541 acggtccgcc gatcgcgctc agcgccgtct tcaggtcgga agagccggag ggatgcttga
     3601 gcaccacttc gtcggcaacc gcagtcagga tgatgccggc aacgatcggc atgtgcagat
     3661 aggtgtaggc ggagcgcgcc agccggccgg agtcgctcgc tttcgagatc agctcggatc
     3721 ccgcttccgc accccggtga aaatagatcc accacatcgc aatcgcggca acgaaggccg
     3781 aggcaaaggc tgcgacgatc tcatgggtcc aggtcaggtc ggcgaaggtc gctccggtga
     3841 cgacgatgga ttcgccaagc gcgatgatga tgaagccggc gcagcgttcc gccatatggc
     3901 cgccttcgac gtaccagtct tcgacggcgg acgcgccgta tttcggaatc cagaaccgcg
     3961 ccaccggccc gacatattcg atcgcaagcg ccacagccca gagcatgagc cgccaatagt
     4021 cctgtgcgaa gccgccgagt atccagaaca ctgcggaggt ggagagccag gtcaggatgc
     4081 ggatcgcatt ccatcgcacg gcggtacgtt cacccgggat cgacgccagc cagaatgcgg
     4141 tgcggccgac ctgcatcgtc gcgtaggcag aggcaaacca caggccgcgc gtctcgaatg
     4201 catgcgggat cgacgtcgac agcacgaggc cggccagcat caaaaggaac agcagaatgc
     4261 ggaccggcgt cagctccgga ttgagccaat tggtgatcca ggtggtgtag acccagaccc
     4321 accagaccgc gagaaacaac aacgtggtct gaaccgcgcc cagcggcgta aatcgattga
     4381 gcagcgtgtg cgagacctgg gtgaccgcaa acacgaacac cagatcgaag aacaattcgg
     4441 cataggtgac gcggttgtgc tggttcggca cgatcgcgcg gaacagcgcg ccgcgttcgt
     4501 tgtcaaccgc catgaacttt cgccccgccg gcccggccgc ttgccgaact agccctccgg
     4561 cacgccggtc tgcagcgcca gcgcatgcag cacgccgccc atctggccgc gcagcgactg
     4621 atagacgatc tggtgctgct gcacgcggga cttgccgcgg aaggattccg aaatcaccgt
     4681 tgccgcgtaa tgatcgccat cgccggcaag atcgcggatc gtcacctcgg catcggggat
     4741 cgctgccttg atcatcgact cgatatcacg ggcgtccatc ggcatcccag tcagtctcct
     4801 gttcgaaaaa ccccttgcac tccccggcgc tagtggtcct ctgcgaagga atcgggccac
     4861 gcgaatcgca gtcagctcgc gtgaccgtga ccgccaaatc tagcgcggac ggctttgaag
     4921 gtcacctatt gtgacactat attcccagtc cgatcattac aaagctgcct gatcgcggag
     4981 caccgcgatc acaacaggct gaccaaaggg cgaaaatcat gaaactcccc ggtcccgacc
     5041 accccatcag catcacccaa aaccccaagc gggtgcgcgt catcgccgat ggaatcgtga
     5101 ttgccgacag cacccgtgca cttacgctca aggaggcccg ctatcccgct gtgcaatatg
     5161 tgcctcgcga agatgcgaac atgggcctga tgcagcggac cgaccgcgtg acgcactgcc
     5221 cttataaggg ggatgccagc tatttcagca tcgttgccaa cggcaagacg ttggacaacg
     5281 cgatctggac ctatgagacg ccctacccgg ccatggccga gatttccggc cacctcgcct
     5341 tttacccgga caaggtgaag atcgaggaag ttgcgtaagg ccgccgcgta cttgcgattt
     5401 tgaggtgcgc acgcgacgaa gcatccggcg gttgtttcgt gaatccgtga gctaactccc
     5461 ggcgaagtac gcagttcctc cgcaatcgca ggcgtgcccc gcgaaggccg cgcagaatgg
     5521 ctcttgccct ccccggttcc cggcggcaag attcggctct acagcaggtg gccgggacgc
     5581 atgatgacat cgatctcgca gaccgcggcg ccggcacaag cgcgggcggt accaaaggca
     5641 aaatcacgca acgcggcgct cgaccgttcg cgcacgttcc tgacgctggt ggtgctgctt
     5701 catcactcgg ttattcccta tacctatttc ggtcataccg atcccaaatc gtggatcggc
     5761 tttgacatga tcgttctcgc caccgacagc ttcttcatgg cgatgttctt ctttctgtcg
     5821 gggctgttcg tttggacggg cctcgctcgc aaaggaccct gggattattt gcgcgaccgg
     5881 ctgatcaggc tcggcctgcc attcgtgatt tgcgcgttca cgctcatccc gctcgcgtac
     5941 tacgcggtcg cgctgcgcca aactcctgat ctgacctttg ccgccttctg gtggaagacc
     6001 attaccgtcg gaccctggcc gagcgggccg atctggttcc tctgggtttt gttcgggttc
     6061 gacctggttg cgagcctcct ctacagtctc tcaccgaaac tgcttgatcc gatcaatcgc
     6121 ctgtcattgc ggggctatga gcagccggcc gcattcttcg cggtcctgct tgccgtcacc
     6181 gcctcgctct atattcccgg gcgagttcac tttggcgcgg gcagttggtt cgaattcggg
     6241 ccgttctcag tccagcacgg ccgtgtcctg ctctatgcga cctacttctt tttcggtgcg
     6301 ggcctgggcg ctgcgaattt cgatcgcgga ctgttgggcg cggacggcca gttggcaaag
     6361 agcagttggg gctgggcgat cttagcgctc gttccgtatt gcctcatgtg ggtgctgatc
     6421 tacatcaagc gcgaattact ggggaatccg atgcagcttc ccgattggta cgaggcgacc
     6481 tacggcctgt tcttcgcttc cttcagcgtg gcgatcatgt tcgtgatttt ggcctatttt
     6541 ctgcgattca agcaatcggg ctggagcatc ctcgatccga tgcagccgga agcctatggc
     6601 atgtttctgg tgcactatcc catcgtgctt tggctgcagt actggctgta cgacttcgac
     6661 atctccgcga tcgccaaggc tctcatcgcc ttcctgctca c
//
LOCUS       DATLJT010001127         1867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2147186,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001127 DATLJT010000000
VERSION     DATLJT010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147186"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VM910_26000"
     CDS             <1..559
                     /locus_tag="VM910_26000"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66010.1"
                     /translation="SIQNPDLAGKRVELLREVVPGLRRLAIMANVELPSAASEMREVQ
                     TAARTLGLDATTFPIRRAEDIAPAFEALKGSADALYVVGDGLVITHRVRINTLALIAR
                     LPTIYPVREYVDAGGFMSYGPNFPDQFRRAADYVDKILRGAKPGEIPVEQPTKFDLVI
                     NLTTAKALGLTIPESFLLRADEVIE"
     gene            556..1536
                     /locus_tag="VM910_26005"
     CDS             556..1536
                     /locus_tag="VM910_26005"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66011.1"
                     /translation="MKRRAFITLLGGMAAWPFAAHGQQSGVPVIGFLSSRSPEDSTHV
                     LPAFAAGLAEGGYVEGQNLAIEFRWARGRYDLLPAMAAELVSRGVAVLTTAGGEPSAL
                     AAKGATSTIPIVFGVGGDPVRLGLVESFSRPGGNATGVTLLTNLMEPKRLGLLRELVS
                     GVPLIGVLLNPNFAQAPVQLQQIEEAARSIDQRITVANVSTDEELQAAFVAFTSQRVG
                     ALLVAADPYFDTRREQIVGLAARHHLPAIYQFREYAVAGGLLSYGVSITDAYRQYGVY
                     TARILKGAKPADLPVLQPTKFETVINLKTAKALDVKISDNLLSLADEVIE"
     gene            1533..1802
                     /locus_tag="VM910_26010"
     CDS             1533..1802
                     /locus_tag="VM910_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66012.1"
                     /translation="MNRREFITLLGGAAVAWPLAARAQQPAIPVIGYLNSGSPESDAS
                     RLTGLRRGLNESGYVEGRNFVIEYRWAGNQADRLPALPPIPCSSG"
BASE COUNT          344 a          577 c          595 g          351 t
ORIGIN      
        1 atcgatccag aatcccgatc ttgctggcaa gcgagtcgaa ctcttgcgcg aggtcgtgcc
       61 aggtttgcgc cggctggcga tcatggccaa tgttgaatta cccagcgccg cttcggagat
      121 gcgtgaggtt cagaccgccg cgcgcacgct cggtctggat gcgacgacct tcccgatccg
      181 acgagccgag gacattgcac ccgccttcga ggcgctaaaa ggcagcgcag acgcactcta
      241 tgtcgttggc gacgggctcg tgatcaccca ccgagtccgg atcaatacgt tagccctgat
      301 cgcgcgactg ccgacaattt accccgtccg ggagtacgtc gatgcgggag ggttcatgtc
      361 ctatggacca aactttccgg atcagttccg ccgagccgcc gactacgtcg acaagatcct
      421 gcgcggagcg aagccgggcg agattccagt cgagcagccg accaaatttg atctcgtcat
      481 caatcttacc acggccaagg cgctcggcct cacgatcccg gaatcgttcc tgctgcgcgc
      541 cgacgaggtg atcgaatgaa gcggcgcgcg ttcatcacgc ttctcggcgg catggcggca
      601 tggccatttg cggcacacgg gcagcagtcg ggggtgcccg taattgggtt cttaagctcg
      661 cgctcgcctg aggactccac acatgttctt cccgcttttg ccgctggttt ggccgagggc
      721 ggatacgttg agggtcagaa cctcgcgatc gagtttcgct gggcgcgcgg tcggtacgat
      781 ctcctgcctg ccatggcggc cgaattggtg agccgcggag tggcggtact gaccaccgcc
      841 ggtggcgagc cgtccgcgct tgcggccaag ggggcaacat cgactattcc catcgtgttc
      901 ggagtgggcg gcgacccagt caggctcgga ttggtcgaga gcttcagccg ccccggtgga
      961 aatgcaaccg gcgtcacgct gctgacaaat ctcatggagc cgaagcggct cgggcttctg
     1021 cgcgagctcg tgtccggtgt gccgctgatc ggcgttttgc tcaacccgaa ctttgcgcag
     1081 gcccctgtgc agctgcagca gatcgaggag gcggcgcgca gcatcgacca gcgcatcact
     1141 gtcgcgaacg tcagcacaga cgaggaactc caagccgcgt tcgtggcgtt cacaagccaa
     1201 cgcgttggcg cgctgcttgt cgcggcagac ccgtacttcg acacgcgtcg cgagcagatc
     1261 gttgggcttg cggcacgaca ccacctgccc gccatctacc aatttcgtga atatgcggtg
     1321 gcggggggat tgctgagtta tggcgtgagc attaccgacg cgtatcggca atatggggta
     1381 tacacggcgc ggattctcaa aggcgcgaaa cccgccgacc ttccggtgct gcagccgacc
     1441 aaattcgaga cggtgatcaa cctcaagacg gccaaggcgc tcgacgtcaa aatctcagac
     1501 aacctgcttt cgcttgccga cgaggtgatt gagtgaaccg ccgcgagttc atcacactgc
     1561 tgggcggcgc ggcggtcgcg tggccgctcg cggcgcgcgc gcagcagccg gccatcccag
     1621 tgatcgggta tttaaacagc gggtcgccgg agtccgatgc ctcccgcctg accggcctcc
     1681 ggcggggttt gaacgaaagc ggctatgttg aaggccggaa ctttgtaatc gaatatcgct
     1741 gggcgggaaa ccaagctgac cgactgccag cgttgccgcc gatcccgtgc agctcgggct
     1801 agtggcgagc ctcaatcggc cgggaggcaa ccttacgggt ttcaacagct ttcacggtga
     1861 aactcgg
//
LOCUS       DATLJT010001128         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_998016,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001128 DATLJT010000000
VERSION     DATLJT010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998016"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VM910_26015"
     CDS             complement(<1..319)
                     /locus_tag="VM910_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HVI66013.1"
                     /translation="MKQGPLIGVKACVFDAYGTLFDFAAAARGCRDVLGDSIDKLTAL
                     WRDKQLQYTWLRAVQSRHADFWQVTADALDFALETLTIEKPGLRDRLMNLYLTLDAFP
                     EVPD"
     gene            complement(325..1089)
                     /locus_tag="VM910_26020"
     CDS             complement(325..1089)
                     /locus_tag="VM910_26020"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HVI66014.1"
                     /translation="MALGLLSLISTRVPALDTATGASSIGEWEATWAKVLTRHVDDSG
                     RIDFDSLRENHADLDLVVAFVAAVNPVTQPRQFPDKHSRLAFYINAYNALAMYRVVQA
                     GVPESLGGLRKFTFFRRRTFAVGGKSISLYKLENDVIRRLGDERIHFALNCMVFSCPR
                     LPRVAFSAAALDGQLDTAARTFIEDTRNVWIDHANREVWLSAIFDFYAADFLAHAPSL
                     IAYVNRYRATQIPADFNVRFLEYHWTVNHRKWAYVR"
     gene            1344..1970
                     /locus_tag="VM910_26025"
     CDS             1344..1970
                     /locus_tag="VM910_26025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI66015.1"
                     /translation="MAGTKQFDHGTVVDRAMMLFWRKGYGGTSIQDLEKATRLRRGSL
                     YNAFGDKQGLFVAALKRYETTVSQERIKQLSNPDPYRAIEGFLETLVTQMSEPSRPRG
                     CLHTNTSLEFPNAPDEVLRIIAERTAGIEGAIYVALRRGQAEGVLDPAADARAVARFY
                     VGVAKGITVLHKVFGDASALRDIVKIAMSRWPRPERKRAGASKRDLRS"
     gene            complement(2100..>2411)
                     /locus_tag="VM910_26030"
     CDS             complement(2100..>2411)
                     /locus_tag="VM910_26030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66016.1"
                     /translation="AAVDWAIAHRVNAGENDLVIMPATFGLGLDPSVRKRDRNPTLFG
                     TGKWSRVLIDATMNLDYDPDPDYGGARFPPTVWPDNDDIAKAYARWSELGLGKPKGTS
                     D"
BASE COUNT          503 a          719 c          740 g          449 t
ORIGIN      
        1 catcggggac ttcgggaaat gcatcgagcg tcaagtaaag attcatcaag cgatcgcgca
       61 ggcctggctt ttcgatcgtc aacgtctcca acgcgaagtc gagagcgtcc gcggtgacct
      121 gccagaaatc cgcgtgccgg ctttggacgg cccgcagcca ggtgtattgc agctgcttgt
      181 cgcgccagag cgcggtcagt ttgtcgatgc tgtctccgag cacatcgcgg cagcccctgg
      241 ccgcagcggc aaaatcgaac agcgtcccat aggcgtcgaa gacgcaggcc ttcacgccga
      301 tgagaggtcc ctgcttcatg cgcgtcacct cacatacgcc catttgcgat gattgacggt
      361 ccaatgatat tcgagaaagc gcacgttaaa atcggcggga atctgcgtcg cgcgataacg
      421 attgacgtag gcaatcaaac tcggggcatg cgcgagaaaa tccgcggcgt agaaatcgaa
      481 gatggctgac agccagacct cgcggttggc gtgatcgatc cagacgttcc gagtatcctc
      541 gatgaatgtg cgtgcggccg tgtcaagttg cccgtcgagg gcggccgcgc tgaacgcgac
      601 acgcggaagt ctcgggcagc tgaagaccat gcaattgagc gcgaagtgaa ttcgctcgtc
      661 gccgagccgg cgaatgacgt cattttcgag tttgtagagg gagattgatt ttccgcctac
      721 agcgaacgtc ctacggcgga aaaaagtgaa cttcctcaag ccgccgagac tttcgggaac
      781 cccggcctga acgacccggt acattgccag ggcgttgtac gcattgatgt agaaggccag
      841 acgcgaatgc ttgtccggga attgtcgtgg ctgcgtgacg ggattgacgg cagccacgaa
      901 cgcaacgacc agatcgagat cggcgtgatt ctctcgcagc gaatcaaagt cgatacggcc
      961 cgagtcgtcg acgtgtcggg tgaggacttt cgcccaagtg gcctcccatt cgccgatgct
     1021 cgatgctcct gtcgcagtat ccaatgcagg aacccgcgtg gagatcagcg agagcagacc
     1081 aagagccatt aaaccactcc gcgagttccg tgcgacgttc ttcccggttc tcgacgatct
     1141 aacgaacgat ggccgcgagg gctgcagtga cggcacgatc ccatagcgag cgtcacgtgg
     1201 aacattgtag ggctatcatg gtacgaccag tcaaagatat ttttagcgcg gtcggccgga
     1261 acggctggcc gcgtcaaaca cattcatgtt acctacggga ggtccgtcgt cggtgccggt
     1321 aaggaaacgc aaaaggcgac actatggcag gcaccaaaca gttcgatcat ggcacggtcg
     1381 tcgaccgggc aatgatgctg ttttggcgca agggctatgg aggaacatcg atccaggatc
     1441 tggaaaaggc cacaaggctt cgccgcggaa gcctctacaa cgcgttcggc gacaagcaag
     1501 gcctcttcgt ggcagcgctc aaacgctatg aaacgacggt gagccaggag cgaatcaagc
     1561 agctgtcgaa tccggatccc tatcgtgcca tcgaaggatt tctcgagacc ctagtaacgc
     1621 aaatgagcga gccgagtcgg ccgcgcggct gtctgcacac caatacgtcg ctcgagttcc
     1681 cgaacgcgcc ggacgaggtc ctgcgcatca tcgccgagcg aaccgccggc atcgaaggcg
     1741 ccatctacgt cgcgctgcgg cgcggtcagg ccgaaggcgt tctcgatccc gccgccgacg
     1801 cgcgggccgt tgcccgcttc tatgtcggcg tcgccaaagg catcactgtc ttgcacaagg
     1861 tgttcggcga tgcgtcggcg ttgcgcgaca tcgtcaagat cgccatgagc aggtggccac
     1921 gtccggagcg caaacgggct ggcgcaagca agcgtgatct tcgcagctaa acttctctgc
     1981 cttgaccggt cgttccagca ttgccgtgcg agcccatcac atgagtcatc gacctggcct
     2041 caggccggaa tattccacgt cgaacgtaac ctttcgccaa cggcgcatct cgacttgggc
     2101 taatcactcg tacctttcgg cttgccgaga ccgagctcgc tccagcgcgc gtaggccttc
     2161 gcgatgtcgt cgttgtccgg ccacaccgtc ggcgggaagc gcgcgccgcc gtagtccgga
     2221 tcggggtcgt agtcgagatt catggtggcg tcgatcagca cccggctcca ctttccggtg
     2281 ccgaacagcg tgggattgcg gtcgcgcttg cgcaccgaag ggtcgagccc caagccgaag
     2341 gtcgcgggca tgatgacgag gtcgttctcg ccggcgttca cccggtgggc gatcgcccag
     2401 tccaccgccg c
//
LOCUS       DATLJT010001129         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_998634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001129 DATLJT010000000
VERSION     DATLJT010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VM910_26035"
     CDS             complement(<1..1794)
                     /locus_tag="VM910_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HVI66017.1"
                     /translation="MSSKHTYVLGINAYDHDVSACLLRDGEIAVAINKERITRKKHDT
                     GFYQKVVDYCLAAEGIALDDVDLAVRNCYVLPVEDLELRLVSQYVPEVMDDEERKQAK
                     NNPLYLSNSNKVVTVSHHLAHAYSAFAACPFDKGVVMVVDGVGNYSSDITEPGQLTEN
                     VNPLARESESYYSFDGSCIETLKKVWLKPVRGFLSDEFYFMPGPGALYSRVSIYIFAH
                     WNKCGEVMGLAPYGRPDAIKPLLEIKSDDVDVPEWGVEFDKPFLPEQERDWEASPSIQ
                     HWKDVAWRVQDDAETLLLKRAIWLRETTGAKNLCMAGGVALNCVANGRIVREAGFDNV
                     WVQPAAGDDGIAIGCAYYGYLALLKKPRSSVMTHAFLGAPYSDEDARDAAEKLLVRLQ
                     TINTPSKNVCRDTARLLAEGHVFAWFQGRSEFGPRALGNRSILADPRKAEMKDKLNKR
                     VKHRQAFRPFAPVVLAERASEIFEGDEESPFMLLVKRVRPEWRDKIPAIVHVDGTARV
                     QTVRQDQNERLYGLLKEFDAITGVPVLLNTSLNVKGEPIVETPADALACFLGTGIDYL
                     ALHDMLIAKNRFHRIFSPLRSAYSEMSSVVQR"
     gene            2149..2385
                     /locus_tag="VM910_26040"
     CDS             2149..2385
                     /locus_tag="VM910_26040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66018.1"
                     /translation="MTKSIRALLSSAAHSVVRGLAVVALVVVWSVGHIGTYALSVVGV
                     STAVLTTTATPADAWWRRWGWRGRRWRRWRRWRW"
     gene            complement(2518..>3354)
                     /locus_tag="VM910_26045"
     CDS             complement(2518..>3354)
                     /locus_tag="VM910_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66019.1"
                     /translation="ISQAEMFDMEYWPLEQAKLGSAAEKPLAGEIAVITGGGGTIGRA
                     TAKAFAAAGAEVALLDCDERAAADAAKAIGAAALAVKCDVTDGACVREAFARVVAAFG
                     GVDIVVSNAGAAWQGRIGEVDEDVLRQSFELNFYGHQRVAQAAVKIMLAQGTGGCLLF
                     NVSKQAVNPGPNFGPYGLPKAATLFLVRQYAVDYGADGIRANAVNADRIRSGLLSDAF
                     IAERSKARGVSEKDYLSGNLLGREVTADDVAQAFLHQALELKTTGDVTTVDGGNIAAA
                     LR"
BASE COUNT          583 a         1060 c         1025 g          686 t
ORIGIN      
        1 tcgctgcacg accgaagaca tctccgaata cgcgctcctc aacggcgaga agattctgtg
       61 aaaccggttc tttgcgatca acatgtcgtg caaggcgaga tagtcgatcc ccgtgccgag
      121 gaaacatgcg agcgcatcgg cgggcgtttc cacgatgggt tcgcccttca cgttgagtga
      181 ggtgttgagc agcacgggaa cgcccgtgat cgcatcgaac tccttgagta atccgtaaag
      241 acgttcgttc tggtcctggc ggacggtctg cacgcgcgcc gtaccgtcga cgtgaacgat
      301 ggccgggatc ttgtcccgcc attccggccg aacgcgtttg accagcagca tgaacgggga
      361 ttcttcatcg ccttcgaaaa tctcgcttgc ccgttcggcc agcaccaccg gcgcgaacgg
      421 ccggaaggct tgccggtgct tcacccgctt gttgagtttg tctttcattt cggccttgcg
      481 cgggtccgcc aggatgctgc gattgccgag cgcgcgcggc ccgaattcgg agcggccctg
      541 aaaccacgcg aacacgtggc cttcggccag cagtctcgcc gtatcgcggc atacgttctt
      601 gctgggcgtg tttatggttt gcagacgaac gagcaatttc tcggcggcgt cgcgcgcatc
      661 ttcatcgcta tagggagcgc cgagaaaggc atgcgtcatg accgatgagc gtggtttttt
      721 cagcagcgcg agatagccgt agtaggcgca accaatcgca atgccgtcat cgcccgcggc
      781 gggctgaacc cagacattgt cgaagccggc ctcccgcacg attcgtccat tggccacgca
      841 gttgagagca acgccgccgg ccatgcacag gttcttggcg ccggttgttt cacgcagcca
      901 gatcgcgcgc ttgaggagca aagtttccgc gtcgtcttga acccgccagg caacgtcttt
      961 ccaatgctga atcgatggac tggcttccca gtcgcgctcc tgctcgggca agaatggttt
     1021 gtcgaactcc acgccccact cgggcacgtc gacgtcgtcg ctcttgatct cgagcagcgg
     1081 ctttatcgca tcggggcggc cgtaaggggc taggcccatc acctctccgc acttgttcca
     1141 gtgcgcaaaa atgtagatcg agacccggct gtacaacgcg ccggggcccg gcatgaagta
     1201 gaactcatcg ctgagaaatc cgcgaaccgg tttgagccag acctttttca gcgtttcaat
     1261 gcacgagccg tcgaagctgt aatagctctc cgactcgcgg gcgagcggat tgacgttctc
     1321 ggtgagttgg cccggctcgg tgatgtcgga gctgtaattg cctactccgt ccaccaccat
     1381 cacgaccccc ttgtcgaagg gacaggcggc gaaagcgctg taggcgtgcg ccagatggtg
     1441 cgacaccgtc acaactttgt tcgagttcga aagatagaga ggattgttct tcgcttgttt
     1501 gcgctcctcg tcatccatga cttcggggac gtattgcgac accaggcgta gttccaagtc
     1561 ctccaccggc agaacgtaac agttgcgcac cgccagatcg acgtcgtcca acgcgatgcc
     1621 ttccgccgcg agacagtagt cgaccacctt ctggtaaaag ccggtgtcgt gcttcttgcg
     1681 cgttatccgc tccttgttga tggcgaccgc gatctcgccg tcgcgaagta aacatgcgct
     1741 gacatcgtgg tcgtacgcat ttatcccaag gacataggtg tgcttgctcg acattcgtgt
     1801 tctgctgatg gacgcgttct aagctggcat tctcggatct acgcgcgggc aagaatgctc
     1861 atttcccgct gcggcaacaa gcccgcaggc tcggctgcga ttgccgacgc ttcgagcgcg
     1921 acgccgctat cgtggcgcgt gctcgcccgg gcaaaaccac agaagtgtgc aaaaccacag
     1981 aagtgtgtaa ggctcaggta agcctgcaac gcgtgtaagc ctcccgacat aaacgaagcc
     2041 gcggatgcgt tcgaggtcgt tggcgacgca gcgttgatct tgaaacggtg gttgaccccc
     2101 gttggccggg agcctaattt tgccgagcgg gcaagagatg gtgacagcat gacgaagtca
     2161 attcgcgcgc tcttgagttc tgccgcccat agcgtggttc gcggccttgc ggtcgtggcg
     2221 ctggtcgtcg tctggtcggt aggtcacatt ggaacttatg cgctcagcgt ggtcggcgta
     2281 tcgactgccg tcttgactac gactgcgacc cccgctgatg cgtggtggcg ccgctggggc
     2341 tggcgcgggc gtcgctggcg ccgttggcgc cgctggcgct ggtaagcggc tctcgagttc
     2401 gaacggcaag gccgaagaac tcgctcatcg aggcgctttc cgtctctatc ttttagggcc
     2461 ctggcacctg ccggggctct ttcattaagg catcgtcgct ccgcggcgcc gagtttccta
     2521 ccgcaacgca gcagcgatgt tgccgccgtc gacggtggtg acgtcgcccg tagttttcaa
     2581 ttccagcgcc tgatgcagga atgcctgcgc cacgtcgtcg gcggtcacct cgcggcccag
     2641 taggttgccg ctgagataat ccttctcgct cacgccgcgc gccttggagc gctcggcgat
     2701 gaacgcgtcg ctgagcagtc ccgagcggat gcggtcggcg ttgaccgcgt tggcgcggat
     2761 gccgtcggcg ccatagtcga ccgcgtattg ccgcaccaaa aacagcgtcg ccgccttggg
     2821 caatccatac gggccgaaat tcggtcccgg attgaccgcc tgcttcgaga cgttgaacaa
     2881 caggcaaccg ccggtgccct gcgccagcat gatcttcacc gctgcctgcg ccacgcgctg
     2941 gtggccgtag aagtttaatt cgaagctttg ccgcagcacg tcttcgtcga cctcgccgat
     3001 ccttccctgc caggcggcgc cggcgttcga caccacgatg tcgacgccgc caaaggcggc
     3061 gaccacgcga gcgaaagcct cgcgcacgca agcgccgtcg gtgacgtcgc atttcaccgc
     3121 cagggcggct gcgccgatgg ccttcgctgc gtccgcggcg gcgcgctcgt cgcagtcgag
     3181 cagcgccacc tcggcgcccg ccgccgcgaa tgccttggcg gtcgcccgcc cgatcgtgcc
     3241 gccgccgccg gtgatgacgg cgatctcacc ggcgagcggc ttctccgccg cgctgccgag
     3301 cttggcctgc tccagcggcc aatattccat gtcgaacatc tccgcttgcg agat
//
LOCUS       DATLJT010001130         3362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3757227,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001130 DATLJT010000000
VERSION     DATLJT010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3757227"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VM910_26050"
     CDS             <1..748
                     /locus_tag="VM910_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI66020.1"
                     /translation="SPEEAAHTVRIALAPGVPAQFHGEFTQRTGVRLLDGWGSTETNF
                     VLGTTVEHQKPGLMGPVFEGFQAGVVDDQGNDVADDAPGELVVKADDPFAFATGYFRA
                     PEKTAEAWRNLWFHTGDRVVRQSDGYFRFIDRLKDTIRRRGENISSFEVEQVLLSHPA
                     VANAAAFPVRSPLAEDEVMAAVLLHPGQHLTEAELIAFCEPRLPYFAVPRYLEFVREL
                     PTTESGKVQKYKLRERGVTERTWDREAART"
     gene            complement(751..1722)
                     /locus_tag="VM910_26055"
     CDS             complement(751..1722)
                     /locus_tag="VM910_26055"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66021.1"
                     /translation="MHAIGLKALGTIIAVLAALGLPAVAQDKVKVGVFPTASSLPYFV
                     AIERGFFKEQNIEPETIRLIGGPANIAAMISNQIEAAIVLVTIEAMNANLKKPGVAMY
                     IGVHSQNKIYQMEQFVTRTGYAAATLKDLKGARIMSAPGPANLVAAKAILAKVGLNEG
                     DYTIDQLDMSQHVNVMTSGAFDAGYTLEPQASTMRKLGVARTLEAGVIAKYILGDEDG
                     NAFAAGCAITSDFIKSRPDVARRFAAVWKKGIDFVNQNPQEARKYLAKNTLTPENVVD
                     TVPMVRYFMAGELTDKHKAEFQKFIDFSTAVGTLPEKVDITKYLQAY"
     gene            1956..2606
                     /locus_tag="VM910_26060"
     CDS             1956..2606
                     /locus_tag="VM910_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66022.1"
                     /translation="MTKLRQWRLVGSAVGEERRFWLDKIIFGLDRVLSRWQSVVEYAH
                     DPTCILRITLGRLDQDFVLADGTAGHPGERFIDLHLWNEHIPAMPKQGASIAWARQMS
                     LCFQHSLRQLARYLASRPDLDDISLVRCTMMFASGERDDQMVRMIGRYGFELIPPATT
                     VTLGERARRLGENIFITLIVLARNQAALRRDTLRRGRTRVFMSRKVLEQRYGGIDL"
     gene            2789..>3362
                     /locus_tag="VM910_26065"
     CDS             2789..>3362
                     /locus_tag="VM910_26065"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI66023.1"
                     /translation="MLFRVGRTFAVVVCVGVVAIDFAAAADIPYVQGPAPAYVPPTPS
                     WAGFYLGGQVGYGMDSVRWRNLGGSAFFSPPNSLTRDRGSGVIGGGQLGYNFQYNRLV
                     FGIEGSISAADFDRSFASPYFPATDVWSSRLTWLTTVTGRVGYGFDSWLPYIKGGFAA
                     GNVETSIQTNPFGVSSQGSAVHSGWTLGGGV"
BASE COUNT          589 a          988 c         1073 g          712 t
ORIGIN      
        1 ctctccggag gaggccgcgc ataccgttcg catcgcgctc gcgcccggtg tcccggctca
       61 gttccacggg gaattcacgc aacgcacagg cgtccgcttg ctcgacggct ggggatccac
      121 ggaaaccaat ttcgtactgg gcacgaccgt cgagcaccag aagccaggat tgatgggccc
      181 ggtgttcgaa ggcttccagg ccggtgtggt cgatgatcaa ggcaatgacg ttgcagacga
      241 tgcacccggc gaactcgtgg tgaaggcgga tgatccattc gcgttcgcaa cgggttattt
      301 ccgtgcgccg gagaaaaccg ccgaagcttg gcgcaatcta tggttccaca caggggatcg
      361 agttgttcgc caatccgatg gctattttcg gtttatcgat cgcctgaagg acaccatccg
      421 gcgccgtggt gagaacatct cgtctttcga ggtcgagcaa gtcttgctca gccacccggc
      481 cgtcgcgaat gcagccgcat ttcccgttcg ttcgccgctt gctgaagatg aagtcatggc
      541 ggctgtcctc cttcatccag gccaacacct cacggaagcc gaattgattg cgttttgcga
      601 acctcgccta ccctatttcg cagtcccgcg atatctcgaa ttcgtgcgcg agctgccgac
      661 caccgaaagc ggaaaggtgc aaaagtacaa gctgcgcgag cgcggcgtta ccgagaggac
      721 ctgggaccgc gaggccgctc gcacttaatt ttagtacgcc tgcagatatt tggtgatgtc
      781 gaccttctcc ggcagggtac cgacggcggt cgagaagtcg atgaatttct ggaactccgc
      841 cttgtgcttg tcggtgagct cgccggccat gaagtaacgc accatcggca cggtatcgac
      901 gacgttttcg ggggtgagcg tgttcttggc gaggtacttg cgcgcttcct gcgggttttg
      961 gttgacgaaa tcgatgccct ttttccacac ggcggcgaag cgccgggcga cgtcggggcg
     1021 gctcttgatg aagtcgctgg tgatcgcgca gccggcggca aaggcgttgc cgtcctcgtc
     1081 gcccaggatg tacttggcga tgacgccggc ttcgagcgtg cgcgcgaccc cgagcttgcg
     1141 catggtcgag gcctgcggtt cgagcgtgta gccggcatcg aaggcaccgc tggtcatgac
     1201 attgacgtgc tggctcatgt cgagctggtc gatcgtatag tcgccctcgt tgaggccgac
     1261 cttggcgagg atcgccttgg cggcgacgag gttggccggg cccggcgccg acatgatgcg
     1321 cgcgcccttg aggtccttga gcgtcgcggc ggcgtagccc gtgcgcgtca cgaactgctc
     1381 catttgatag attttgttct ggctgtgcac gccgatatac atggcgacgc cgggtttctt
     1441 gagattggcg ttcatcgcct cgatggtcac gagcacgatt gccgcctcga tctgattgct
     1501 gatcatcgcg gctatgttgg ccgggccgcc gatgagccgg atggtttcgg gttcgatgtt
     1561 ctgttccttg aagaagccgc gctcgatcgc cacgaaatag ggcagcgagg acgcggtcgg
     1621 gaacacgcca accttcacct tgtcctgcgc gacggccggc aggcccagag cagcaaggac
     1681 ggcgatgatc gtccccagcg ccttcaaccc gattgcgtgc atcgcatcac ctccgtcgtt
     1741 ccatgagcca ccaatgtctc gcctgcactt gccataactg aggcgggcgg ctcgatccat
     1801 caggacgagg ccgcggccga catcactaac ttcagcgtca ttaacttcag cgtcatgggg
     1861 ctgttcgtgg cttggggact tggtgcaggt tggaagccgg tgcagactag tggattgtaa
     1921 tattgcagca agcatgccgt taggcatccg atgccatgac taaattgcgg caatggcgac
     1981 tggtcgggtc ggcggtggga gaagagcgtc gtttttggct ggacaagatc atattcgggc
     2041 tggaccgcgt gctgagccgt tggcagtccg tcgtcgaata tgcccatgac ccgacctgca
     2101 ttttgcgtat caccctcggt cgtctcgatc aggacttcgt gcttgcggat ggcacggcgg
     2161 gtcaccccgg cgagcgcttc atcgatctac atctgtggaa cgagcacata ccggcaatgc
     2221 cgaagcaagg tgcatcgatt gcatgggcgc ggcagatgag cctttgcttc cagcattcgc
     2281 tgcggcaact cgcgcgctat ctcgcctcgc gtcccgatct cgacgatatt tcccttgtgc
     2341 gttgcaccat gatgttcgct agcggggagc gcgacgacca gatggtgcgc atgatcggcc
     2401 gctacggatt cgagctcatt ccgccggcga caacggtgac gttgggtgaa cgcgctcgcc
     2461 ggttgggcga aaacattttc atcaccctca tcgtgctggc gcgcaatcaa gcagcgctgc
     2521 ggcgagacac gctgcgccgg ggccggacac gggtcttcat gtcgcgaaag gtgctcgaac
     2581 aacgctatgg cggcatcgat ctgtagcatc ggccgacccg cccatcctga ccgacgacaa
     2641 cggtgctttg ataaggtccc gctcgagtga agcgccgcag aatttattaa ccttgtcgga
     2701 aagcagacgt atcgaagccg cgctttcgcc aacgtcgcgg gcaagtttcc gcaaaatctc
     2761 gagttggggg cgaccaggaa gaggccatat gcttttccgc gtcggtcgaa cgttcgctgt
     2821 cgtcgtttgc gtgggcgttg tggcgattga ttttgccgca gcagccgata ttccctacgt
     2881 gcaaggtccc gcgcctgcgt acgtgccgcc gacgccgagc tgggccggtt tctatctggg
     2941 tggccaggtc ggctacggca tggatagcgt ccggtggcgg aatttgggtg ggtcggcgtt
     3001 tttttcgccg ccgaattcgc tgacgcgcga ccgaggaagc ggagtgatcg gcggcgggca
     3061 gctcggctat aactttcagt acaaccggtt ggtgttcggc atcgaaggat cgatatcggc
     3121 cgccgatttc gaccgcagct tcgccagccc gtatttcccg gcaacggacg tgtggtcctc
     3181 gcggctcacc tggctcacca cggtgaccgg ccgcgttggc tacggcttcg acagctggct
     3241 gccctacatc aagggcggct tcgccgccgg caacgtcgag accagcattc agaccaaccc
     3301 gtttggcgtc agctcgcagg gatcggcggt gcactcgggc tggacgctgg gcggcggggt
     3361 cg
//
LOCUS       DATLJT010001131         3185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_311082,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001131 DATLJT010000000
VERSION     DATLJT010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311082"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VM910_26070"
     CDS             complement(<1..838)
                     /locus_tag="VM910_26070"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI66024.1"
                     /translation="MVAAVASAFGIAHDYGYLHAPILTGSPGGQYHALATRLADRAKR
                     EHGTLTVIPTAGSIENVSRLANARGGCAEKFAVIQDGTPVPADARLELLGRLPEPESL
                     LLLGRPGHAFHSFADLRGALIGIGPEGSGTAYLMQQLFEDPDLQELDVHLSHHELLEQ
                     AELVARSKLDLAALVIQEDAELLRTIIRQHGLDIISPQDLQGLIARYPWLSLGRIPAG
                     RYDVVRPLPAVDKQVARLGTLVIASPCAQRADRIALLMLLAAELPGFVRGNPPSSTSS
                     ATV"
     gene            complement(1239..1415)
                     /locus_tag="VM910_26075"
                     /pseudo
     CDS             complement(1239..1415)
                     /locus_tag="VM910_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
     gene            complement(1496..2872)
                     /locus_tag="VM910_26080"
     CDS             complement(1496..2872)
                     /locus_tag="VM910_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HVI66025.1"
                     /translation="MPPNVTARRSRAFPSTPTGTLAERYDRGVGLRRKTPRKKHADLL
                     GPTDRDPVAILARSDRTRVPELVPVRYERMLTSPFAFLRGAAAVMSEDLRHQPAAGIS
                     VQACGDCHLMNFGAFATPEENILFDINDFDETLPGVDFTVDLKRLAASVAVAALAAKF
                     SNKRARATAAATVEAYRTRMRALAKLSPLEIWHSRIELWSEIKNIEDRVLRRQLHAIL
                     TKAGKRLEQDDNFPHLVGGKQARIVDKPPLIYHFTRKRDARHRVNAERMFASYQQRLP
                     PERLCLFDRYSLSDIAFKAVGVGSVGTFCAIGLFASGDGAPLFLQVKEAGKSVLECLG
                     PKFDGHPGRRVVEGQHIMQAASDVFLGWTQDEASGRHFYVRQLKNRRLGSISELVEEN
                     ALVNYARLCGRTLARAHARSADPAVLAGYMGKSDAFDDALASFAMAYAVRTRKDYDQL
                     AKSKRKAA"
BASE COUNT          584 a          974 c          990 g          637 t
ORIGIN      
        1 ataccgtagc ggagcttgtg gaactcggag gattgccgcg gacgaagccc ggcagctcag
       61 ctgcgagtaa catgagaagc gcgatccggt cggctcgctg agcacacgga ctagcgataa
      121 ctagggttcc gaggcgcgca acttgtttgt ccactgcggg gagcgggcgc accacgtcat
      181 agcgcccggc tgggatacgg ccaagactga gccacgggta gcgagcaatt aagccctgga
      241 ggtcctgggg cgatataata tccaagccgt gctggcgaat gatggtgcgt agcagttcgg
      301 cgtcttcctg aatcacgagg gcggcgaggt cgagtttgct ccgggccacc aactcggcct
      361 gttcgagcag ttcgtgatgc gagagatgga catccaattc ctggagatcg ggatcctcga
      421 agagttgctg catcaggtac gccgtgcccg acccttccgg cccgatcccg atcaatgcgc
      481 cgcgcaaatc ggcaaagctg tgaaaggcat ggcccggtct cccgagcagc agaagagatt
      541 ccggttccgg gagccggcca aggagctcga gccgggcatc ggccggcacc ggcgtgccgt
      601 cttggatgac ggcgaatttt tcggcgcacc ctccccgagc attggccaag cggctgacgt
      661 tctcgattga gcccgcagtt ggtatgacgg taagagtacc gtgctcgcgc ttagcccggt
      721 cagccagacg cgtggccaac gcgtgatact ggcctccagg agagcccgtc agaatcggcg
      781 cgtgcaggta gccatagtcg tgagctatgc cgaatgctga agcgaccgct gcgaccaccg
      841 ccgccgcgac cagaagaagc gaagcgcgga caagtccttt gccgcgccag gcgacaccca
      901 aacctgcgaa cggatttcgc ggttgcatgg tgagatcgac gcctctccct cctggcagcg
      961 ggagctattg gtttcgatca gctcaacgag gctaggccat tgtcccgttc gcgtttttgc
     1021 tcaccaagcg ggcaccgtac gacgccgctt gttattggcg cgcttgcctg tcaccttgcg
     1081 ataaatagcc gtctcggctt gctgctgcgc aacaccggcg gagatctcgg acttttgccg
     1141 attgatacga ctaaggccga tatagcaatc ggtccgtgct ggcccagcag gaaacatctc
     1201 gcaatttact tgcgccactg caaggccaag cgagcctgat tgggaatcag gtcggcacga
     1261 cgctggtact ggttctgcac atccgaccac ttcgccttcg cgctttcgtc ataggtcggg
     1321 atgttgttga cgccgcagcc cgaaagcagc aatccggcga gtacggccaa aagcgggatg
     1381 cgggtgcggc ggggaacgca tagtgtatgg ctcatgcgat agctcccgtg gtcttctgat
     1441 tcgcccgaga ctgcgttgcc gatatcgcgc gatgatccct ttgtccctat cgttgctagg
     1501 cggctttgcg cttcgatttc gcgagctgat cgtaatcttt tcgtgtgcga accgcgtagg
     1561 ccatcgcgaa agatgccagc gcatcgtcaa aggcgtcgct ctttcccatg tatcccgcga
     1621 gcacagctgg atcggccgaa cgagcatgcg cgcgggcgag cgtgcgcccg cataggcgcg
     1681 cgtagttgac gagggcattc tcctccacga gttcgctgat tgagccgagc cgccggttct
     1741 tgagctggcg cacgtagaaa tgtcggcctg acgcctcgtc ttgcgtccat cccaggaaga
     1801 catcgctcgc cgcctgcatg atgtgctgac cttcgacgac ccgccggccg ggatgaccgt
     1861 cgaatttggg tccgaggcat tcgagtaccg attttcccgc ttctttgacc tgaaggaaga
     1921 gcggagcgcc gtccccgctc gcgaacagac cgatcgcgca gaacgtcccc acgcttccca
     1981 ccccgaccgc cttgaaggct atgtcactga gggagtagcg atcgaacaag cacagccgct
     2041 ccggcggaag acgctgttgg taagaggcaa acatgcgctc ggcgttgacg cggtgccgcg
     2101 cgtcgcgctt tcgcgtgaaa tggtagatca gtggcggctt gtctacgatg cgcgcctgct
     2161 tgcccccgac cagatgcggg aagttgtcat cttgttcgag ccttttgccc gccttggtga
     2221 ggatggcatg gagctgtcgc ctaagcacgc ggtcctcgat atttttgatt tcgctccata
     2281 gctcgatccg gctgtgccaa atttcaagcg gtgagagctt ggcaagggcg cgcatgcggg
     2341 tgcggtacgc ttcgacggtc gcggccgcgg tcgcgcgtgc ccgcttgttc gaaaacttcg
     2401 ctgccaacgc ggccacggcg acgcttgcag ccaatctctt gaggtcgacc gtgaagtcga
     2461 cgccgggcag cgtctcgtcg aagtcattga tgtcgaaaag aatgttttcc tccggggtgg
     2521 cgaaggctcc gaagttcatc agatggcagt cgccgcaggc ctggacggaa attcccgcgg
     2581 ccggttgatg cctcaggtcc tccgacatca ccgcggccgc gccgcgtagg aaggcgaatg
     2641 ggcttgtaag cattcgctca tagcgaaccg gtaccagctc cggcacgcgc gtgcggtcgc
     2701 tccgcgccag gatcgccacc gggtcgcgat cggtgggccc gagcagatca gcgtgctttt
     2761 tgcgaggggt ctttcgccgc agaccgacgc cccgatcgta acgttcggca agcgtgccgg
     2821 tcggcgttga gggaaatgcc ctgctacggc gagctgtcac attaggaggc atcgttgccg
     2881 ctccaattct gtcagaaaca acaacgcacg ccgcgccggt gctgcggccg gagggtgctg
     2941 tttcaactgg ctgcgcggat gcgaacctac atcatccacg ctacctcctg ctcgttcggc
     3001 aaccgcccgt ttggcgtgag ctgatcgatc aagtctggca gatgctcacc catcatgcac
     3061 catggcttta cctcttacca gctcggtcgc cactctcgcc ggtcatcgcg ccactgttgt
     3121 ccagatcccg agtgccaacc ccattgttgc tgcgctcccc acggttgccc tgaccaggag
     3181 ttagt
//
LOCUS       DATLJT010001132         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_767739,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001132 DATLJT010000000
VERSION     DATLJT010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767739"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VM910_26085"
     CDS             complement(<1..294)
                     /locus_tag="VM910_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI66026.1"
                     /translation="MSAALLTIEHLEVRYGDLIGVSDVSLEVAAGSVVALLGSNGGGK
                     TTTLNAIAGLIPVHSGRIAFRGEEIAGQSAFAIVRKGLALSPEGWRLFVQQSVE"
     gene            complement(342..1115)
                     /locus_tag="VM910_26090"
     CDS             complement(342..1115)
                     /locus_tag="VM910_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66027.1"
                     /translation="MLLEARDITKAFGSFKAVDSASVTLERGDILGLIGPNGAGKSTF
                     FNCLTGDLMSTSGRVFFEGRDITTLAPEARAQLGLARTFQVPLTFEGLSVLENVMIGA
                     FLRHPHRAEAEKRARAVLERVGMARLANAPARSLGTPGRKRLEIARALATEPKVLLLD
                     EAMAGLTQREVQLAIDLVRDIHRSGITLVIVEHIMEVIMSLASRVMVFHQGKEIARGS
                     PREVTSNPAVIAAYLGTRAAKAAAGHTPIELMGGPVPPT"
     gene            complement(1105..2100)
                     /locus_tag="VM910_26095"
     CDS             complement(1105..2100)
                     /locus_tag="VM910_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66028.1"
                     /translation="MRRLLWPLLLLALVIVYPLVLSTPFEQRLGALVLLYAIAASSWN
                     IVGGYAGQVSVGHVVFFGCGAYAAMGAYAHFGLSPLVGLPAGIVLAVTIAAVIGVPTL
                     RLSGHYFSMATIAVAELIRLVVTNTNYLGAAVGLSGPPVPRNVFDVSFISALPYYYLF
                     LVILAITLLITWWMANSRMGFYLRAIKDSERAARSLGVPASRAKLHAYMLSAGLTSVA
                     GALYAMMFGFVDPESGLGILISVKMLIMAALGGAGLLFGPLVGAAILVPLEELSNSLL
                     GGQGAGLTFVVYGAIIVLIARFQPGGILTLANRLWEKRAKPEKAGASAAGASHAS"
     gene            complement(2100..2963)
                     /locus_tag="VM910_26100"
     CDS             complement(2100..2963)
                     /locus_tag="VM910_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66029.1"
                     /translation="MSFVLVQVIVGGLLLGAVYALFSAGLTLVWGMMNIVNFAHGDFV
                     MLGMYVAFVVFTLFGVGPAAGAPIAMLLLATLGVVVYFALIRGIMKGPMLAQILGTFG
                     LALLLRYSVFAWFGANFLTLPQDIVGGTLVFAGLRIEASRLLAGIVALLVTLGLHLLL
                     TRTTLGSKMLAVAEDATAAQLMGIRPDTMQAIAWAIAAGATGLAGALIATFFYIVPTV
                     GETLGIVAFVTVSLGGFGSVPGALIAGLLIGVIESLSGYLIGAVYQDIVVYALFVSFL
                     WFRPQGLMGKM"
     gene            complement(3041..>4162)
                     /locus_tag="VM910_26105"
     CDS             complement(3041..>4162)
                     /locus_tag="VM910_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66030.1"
                     /translation="SAGIHAKAAIEVALDIINNAHPELGALPLAKNAGLAGLGGAKVE
                     VVFADNQGSPATGQNQALRLISEEKVVALTGAYQSGITLTTSAIAEKYGIPFLTGESV
                     AANLTERGFKWFFRTTPIATDFVRVYSDFLADMKAQGMKTDNVALVHDNTEYGTSVAN
                     TIATGFKEKGFNIIDVAYASNATDVQSQVLQLKEKKPDVVLMISYTSDAILFAKTMQA
                     QDYKPPLLLADDAGYSDPSFIKAVGKMSQGAFNRSAWSVGAPGSATALIAEMYKKKSG
                     DDMDDTAGRQMQGFFVLVDAIDRAGSTEPAKIQAALKATDLKPEQLMMGYKGVKFDDK
                     GQNILASAIIIQLQDGENYFAVWPKSNAEKAPILPYKGW"
BASE COUNT          876 a         1315 c         1265 g          706 t
ORIGIN      
        1 ctcgacgctt tgctgcacaa acagccgcca gccttccggc gacaatgcaa gccccttgcg
       61 cacgatcgcg aacgcgcttt ggcctgcgat ctcctcgccg cgaaacgcga ttctgccgga
      121 atgaaccggg atcaggccgg cgatcgcatt cagggtcgtg gtcttgccgc caccattgga
      181 gcccagcaac gccaccacgc tgcccgcggc cacctccaac gacacatcgg agaccccgat
      241 caggtcgccg tagcgaacct cgagatgttc gatcgtgagc agggccgcgc tcatctcatt
      301 agtgcccgct tttccgaagt tcgttacaca tcgcggcatc ctcaggtcgg cggcacaggc
      361 ccgcccatca attcgatcgg ggtgtgaccg gcggcggcct tggccgcgcg ggtgccgaga
      421 taggcagcaa tcacggccgg attgctcgtg acctcgcgcg gggacccgcg ggctatttcc
      481 ttgccttggt gaaagaccat cacgcggctg gcgagcgaca tgatcacttc catgatgtgc
      541 tcgacgatga cgagggtgat gccggaacga tggatatcgc gcacgagatc gattgccagt
      601 tgcacctcgc gctgtgtgag gcccgccatg gcttcgtcca gcaacaggac cttcggctcg
      661 gtcgcgagcg cccgagcaat ctccagccgc ttgcggccag gcgtgccgag cgagcgggcc
      721 ggcgcattcg caagcctggc catgccgacc cgctccagca ctgcgcgcgc tcttttttcc
      781 gcctcggcac gatggggatg gcgcaggaac gcgccgatca tcacattctc gagcaccgaa
      841 agcccctcaa aggtcagcgg cacctggaag gtacgcgcaa gccccaattg cgcgcgcgct
      901 tccggcgcaa gtgtggtgat gtccctgcct tcaaaaaaga ccctgcccga ggtcgacatc
      961 aggtcgccgg tcaggcagtt gaagaaggtc gacttccccg caccgttcgg tccgatcagg
     1021 ccgaggatgt cgccccgctc aagcgtcacc gaggcggagt cgaccgcctt gaaacttccg
     1081 aaggcttttg tgatgtcgcg cgcttcaaga agcatggctt gctcctgccg cactcgctcc
     1141 cgccttctcg ggcttggcgc gcttctccca tagccggttg gcaagcgtca ggatgccgcc
     1201 cggctgaaag cgcgcgatca gcacgatgat cgcgccatag acgacaaaag tgagccccgc
     1261 gccctgcccg ccgagcagac tgttggaaag ttcttccagc ggcaccagga tcgcggctcc
     1321 gaccagcggc ccgaacagca gcccggcccc gccgagcgct gccatgatca gcattttcac
     1381 cgaaatgaga atgcccaggc ccgattccgg atcgacaaat ccgaacatca tcgcgtagag
     1441 cgcgccggct accgaggtaa gccctgcact cagcatgtag gcatgaagct tggcgcgact
     1501 cgcgggcaca cccagggaac gcgccgcgcg ctcggaatcc ttgattgcgc gcagataaaa
     1561 tcccatacgg ctgtttgcca tccaccaggt gatcaaaagc gtgatcgcga ggatcaccag
     1621 gaacaaatag tagtacggaa gtgcagagat gaaggagaca tcgaagacgt tgcgcggcac
     1681 gggcggacca ctaagaccaa cggctgcgcc gagatagttg gtgttggtga ccacaagcct
     1741 gatgagttcg gcgactgcga tcgtcgccat gctgaaataa tgccctgaca gccgcaaggt
     1801 cggcacgccg attacggcgg caatggtcac ggccagcacg atgccggcgg gaagcccgac
     1861 caacggcgac agcccgaaat gcgcgtaggc gcccatcgcg gcataggcgc cgcagccgaa
     1921 gaacacgaca tggccgaccg aaacctggcc ggcatagccc ccaacgatat tccagcttga
     1981 agctgcaatc gcgtagagga ggaccagcgc gccgagccgc tgctcgaacg gcgtagacag
     2041 gacgagcgga tagacgatca ccagcgccag cagcagcaag ggccagagca agcggcgcat
     2101 cacatcttac ccatcaggcc ttgcggccgg aaccacagga aggagacgaa cagcgcatag
     2161 acgacgatgt cctggtagac ggcgccgatt agatatcctg agagcgactc gatgacaccg
     2221 atcaggagac ccgcgatcaa cgcacccggc acgctgccga acccgccgag cgacacggtg
     2281 acgaaagcga cgatccccaa ggtctcgccg accgtcggca caatgtagaa gaaggtcgcg
     2341 atcagcgcgc ccgccaagcc agtggcaccg gctgcgatcg cccaggcgat ggcctgcatg
     2401 gtgtcgggcc tgatccccat gagttgggct gcggtcgcat cttcggcaac ggcaagcatc
     2461 ttggatccca gtgtggtgcg cgtcagcaga agatgcaggc caagcgtcac gagaagcgcg
     2521 acgataccag ccagtagccg cgacgcctcg atccgcaggc ctgcgaaaac gagggtgccg
     2581 ccgacgatat cctgcggcag cgtcaaaaag ttcgcgccga accaggcaaa caccgaataa
     2641 cgcagcaaca acgcaagtcc gaaggtgccg agaatctgcg cgagcatcgg ccccttcatg
     2701 ataccgcgga tcagggcgaa atagaccacg acgccgagcg ttgcgagcag cagcatcgca
     2761 atcggcgcgc cggcggcagg gcccacgcca aacagcgtaa agaccacgaa ggccacatac
     2821 attccgagca tgacaaaatc gccatgggcg aaattgacga tgttcatcat gccccagacc
     2881 agggtcagac cggcggaaaa cagcgcgtaa accgctccca gcaaaagtcc gccgacgatc
     2941 acctggacaa gaacgaagga cattgaaccg cgcgccgttg cgaggaagga tgccgccgga
     3001 tgcaaacaaa ggggcggaac ttgcgtcccg tcctcccaaa ttaccagccc ttgtacggca
     3061 ggatcggtgc cttctcggca ttcgacttcg gccagactgc gaaataattc tcgccgtcct
     3121 gcaactgaat gatgatggcg gaggcgagga tgttctggcc cttgtcgtcg aacttgacgc
     3181 ccttgtaacc catcatcaac tgctcgggct tgaggtccgt cgccttcagg gccgcctgga
     3241 ttttcgctgg ctcggtcgag cccgcgcggt cgatcgcgtc cacaagcacg aagaagccct
     3301 gcatctggcg accggccgtg tcgtccatat cgtcgccgct ctttttcttg tacatctcgg
     3361 cgatgagggc ggtcgccgag cctggcgctc cgacgctcca ggcggagcgg ttgaacgcgc
     3421 cttgcgacat cttgcccact gccttgatga aggacgggtc ggaatagccg gcgtcgtcag
     3481 cgagcaagag cggcggcttg tagtcctgtg cctgcatggt cttggcaaac agtatggcgt
     3541 ccgacgtgta ggaaatcatg agcacgacgt cgggcttctt ctccttcagt tgcagcacct
     3601 ggctctgtac gtcggtggcg ttggaggcat aggcgacgtc gatgatgtta aaaccctttt
     3661 ccttgaagcc ggtggcgatc gtattggcga ccgaggtacc gtattcggtg ttgtcatgca
     3721 ccagcgcgac attgtcggtt ttcatgcctt gcgccttcat gtcggccagg aaatcggaat
     3781 agaccctgac gaaatcggtc gcgatcggcg tcgtgcgaaa aaaccatttg aagccgcgct
     3841 cggtgagatt ggccgccacg gattcgccgg tcaggaacgg gatgccatat ttctcggcaa
     3901 tcgcgctggt cgtcagcgtg atcccggatt gataggcccc cgtcagcgca accaccttct
     3961 cctcggagat cagccgcaag gcttggttct gtccggttgc agggctgccc tggttgtcgg
     4021 cgaagacaac ttcgactttg gccccgccca aaccggcaag gccggcattt ttcgcaagcg
     4081 gaagggcgcc taattcaggg tgcgcgttgt tgatgatatc gagcgcaact tcgatggcgg
     4141 ctttggcatg gatgccggcg ct
//
LOCUS       DATLJT010001133         9348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_542124,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001133 DATLJT010000000
VERSION     DATLJT010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542124"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VM910_26110"
     CDS             complement(<1..1517)
                     /locus_tag="VM910_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI66031.1"
                     /translation="MAQPAKPKPTGKLLRKAKTGGLRRSRKASLSGSRRNTGSGRDIF
                     RALRPNAANFATLTPISFLPRSAEIHPERIAMIHGADRYNYRQVYERARRLASALARA
                     GIRPGDTVSAMLPNVPAMVEAHYGVPMLGAVLNTINTRLEPATIAYILQHGEARVLIT
                     DREFAAQVGPALAKLERRPLVIDVDDALYSGSGERLGRIEYEEFIAKGDPSFAWTSPA
                     DESSAIALNYTSGTTGNPKGVVYHHRGTFLECVGNIIAWPLPPKPVYLWTLPMFHCNG
                     WCFPWSVVAMGGTHICLRKVDPALIFSMIAEHRVTHMCGAPTVLNLLTSAPAEQRRRF
                     DHLVDIQTGGSPPPAKVIKGMEELGFRVTHIYGMTELQGPSTLCVPQDGWGKLPLEER
                     AALTARQGVRYPVVEGHMVADPKSLRPVPQDGKTIGEIMVRGNTVMLGYLKEPKATAD
                     IFRGGWMRTGDLAVEHPDGYVEIKDRAKDIIISGGENISSVEVEIALYKHPAVQLA"
     gene            1756..2952
                     /locus_tag="VM910_26115"
     CDS             1756..2952
                     /locus_tag="VM910_26115"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66032.1"
                     /translation="MRVLTAAFATLLAVPALFASEGAVAQDRTVKITGFGAKSGVVRV
                     FGINSEAAMKAGAEEINKAGGVTLGDGSKAKLTVEFLDDRCNAEEGISVVRRIASTDA
                     FVAVGPTCSNVAESLFGILQKKVGDASDSGLQFPIIADVAAKGGLAKISEWSFRNVPS
                     EFEMYKSLFAWIKSQHPDLKTIFGGVEKDFAHSNATYGVMKAQADANGFQLLGTSEWL
                     LNDTTFSTQVRDMKRANADIVAISAHPFTTCGVLKEMERQGVKPKLLIGLTSSSSIET
                     LQGCAKQAEGIIIPTSFAPVTPEAKKAAENVAKFGGSADLHSAAAYEIMFILKDVIEQ
                     QKILAKPDTVQADRNKMREGLAALKETNGLLGKVGRTADREAIKPFLYVHAKDGSWLV
                     LHKPSS"
     gene            3061..3951
                     /locus_tag="VM910_26120"
     CDS             3061..3951
                     /locus_tag="VM910_26120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66033.1"
                     /translation="MDVLDLIDAVQSVADGVLFGATYALLGIGFTLVFGVMHKINLSY
                     AAASIGAAYASLLIVAGIKAPAILVFLASAAAGGVIGGLVYLVGFKFIPLANPLATLM
                     STVGLLLLIDEIIVHTTAGVPLNYPALFSDVIVSAGPFNLRGDLMFVFALSCACMVVL
                     LLLLYRTRLGLATRAVSQQPTASQLCGISITRVNVVTFVITGVVGGIAGAMTGAAIGM
                     LSPLLSLPLTVKGLIVTVIGGLGSIPGAIIAGLVVGGLENAFQFFRGVSERDLYIMLL
                     LFAFLIFRPGGMFAMPVGRD"
     gene            3969..4880
                     /locus_tag="VM910_26125"
     CDS             3969..4880
                     /locus_tag="VM910_26125"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66034.1"
                     /translation="MDFYLGLMQIVGVHTLLGLSAYCVLLTGQVSLAQAGFFAIGAYV
                     AGMLTVLAQWPLVPALGASAVVAGVMACAVGFPALRVKGLMLVVATIAFGEAVRLFFF
                     NFTYQVQAGNLKLGPHGAEGFRQIRYFPEHGWTTLDVMLFIWAVVALVMAGVWWLDRS
                     RAGAVLRAVGEDELAAQSAGINITAVKVSAMTIGGVIAGIGGGIFAHYTTHIEHLQFG
                     VVLATFAIAYPILGGLSNVFGTLLAVVFVQGFLIEGLRFMGDWRNLLFGGLIVLAMNV
                     RPRGLLDAGAVLALRRLIVPRKDRNGA"
     gene            4873..5622
                     /locus_tag="VM910_26130"
     CDS             4873..5622
                     /locus_tag="VM910_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66035.1"
                     /translation="MLDLQGLTKHFGGVRAVDGIDLAVTRGEIVGLIGPNGSGKSTIV
                     NLICGLLPPTAGRVVFNGADISRLPPHARVTLGIARTFQNIRLFGQLTVWQNLWVAQN
                     SGEQRNQNFLRRWFGGSNGAWEEINRTLEFFDLAHKRDELAANLAFGEQRRLEFARAL
                     AAKPVLLLLDEPAAGMNAEEVDQLDSRIRKLRQDGITVLLIEHHVELVMSVADRVAVL
                     NFGQKIAEGTPAFVQADPAVREAYLGSAAVA"
     gene            5631..6350
                     /locus_tag="VM910_26135"
     CDS             5631..6350
                     /locus_tag="VM910_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66036.1"
                     /translation="MLDISDLVTAYGKIDALKGVSLQVETGKITCLLGPNGAGKTTLM
                     MTIAGLLHPRRGSIRLAGTELVGLSPARIVAHGIALVPENRLVFPQMSVRENLLAGAY
                     QRNDKVSIDADLKRMYALFPRLNERTEQLAGTLSGGEQQMLAVARALMSRPRVLLMDE
                     PSLGLAPLVVTEIFNIVRDLNRDGTTIFLVEQNAHMALQVAHRFLLMEQGRITFRGMP
                     GQLAEDEVVQRAYLGTRRAAG"
     gene            6371..7261
                     /locus_tag="VM910_26140"
     CDS             6371..7261
                     /locus_tag="VM910_26140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66037.1"
                     /translation="MIDLVQNTLDGVMIGSSYGLLALGFTLIFGVMRRLDLAYGPSIM
                     IGGYFGTVLYLYYGAGPLAVASAVVLGAVIAGIYVERLCFAPMKAGAAIASMVSSFAI
                     WMQLEQAAILVLPRHTYPFPPLATVAPLEFGPFLVRVDHLIMLVCALALAAAVHLALY
                     HTRFGLALRAIIGSPVAAHLVGINVRTAVLLTFAGASAVGGAAGFLVLAVDQQVTPMF
                     GMWATLKGLIAMMIGGLGSVPGAVVGGLLLGIVEVNSQWYFGPQIRDLVAYVVLFAFL
                     VVRPRGLLGRAPEPAMPPKA"
     gene            7265..8206
                     /locus_tag="VM910_26145"
     CDS             7265..8206
                     /locus_tag="VM910_26145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66038.1"
                     /translation="MDDYLIGVLSNIGVISFVALSAYLLLLTGEISFGQQAFFAIGAY
                     AAGIATALWGWSLAAALVWGAAVSGGAALLVGVPTLRLHGLYFAIATLAFAEMVRILF
                     ELFRYQISIDGELIGPNGTDGFRNIRYIFDHNIDAFQFLLLIYSLLAAAMLGLLLLER
                     SRLGVIFRMIGQDETLAELQGIPVARRKVLAAGIAGTLAGIGGGLYAHLTTYVEPRIF
                     DIMLGVHSLAYGLIGGLGTAFGPLLGVLIDIGLLESIRALAGYRMIIFGGLVALLLIV
                     RPRGLLDEMVVHKIGRGWRRVSLWARRSSLKPRGNSA"
     gene            8224..8646
                     /locus_tag="VM910_26150"
     CDS             8224..8646
                     /locus_tag="VM910_26150"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HVI66039.1"
                     /translation="MKSATYVLATLAMLAPCGLENAHAQIRAKADVSCQPAEKLQYDC
                     IIKLANARTSEPLSGLTLTVGADMPSMPAAHNMRPVKATEEQDKGTYRARIALEMYGD
                     WALRLELSGTVRDRVVKLLRFESDRVGEATPAPGRHRH"
     gene            complement(8675..8815)
                     /locus_tag="VM910_26155"
     CDS             complement(8675..8815)
                     /locus_tag="VM910_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66040.1"
                     /translation="MSARRACRHDQAAVRLAGECNDGALDLAGVARMDRAQLDPPSDG
                     AT"
     gene            8856..9107
                     /locus_tag="VM910_26160"
     CDS             8856..9107
                     /locus_tag="VM910_26160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66041.1"
                     /translation="MPNVYPFRYYPSHGGLASYGPDQIDQYKRAAGYVDRILKGEKPS
                     DLPVQTPTKFELVINLKTAKAIGFELPPTLLARADEVIE"
     gene            9104..9286
                     /locus_tag="VM910_26165"
     CDS             9104..9286
                     /locus_tag="VM910_26165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66042.1"
                     /translation="MNRREIIMLIGGAAAWPLAARAQQGERVRRIDVLMSAAAADLEG
                     QACIAAFRRAGSDSIL"
BASE COUNT         1576 a         2876 c         2959 g         1937 t
ORIGIN      
        1 gcaagctgga ctgccggatg tttgtagagc gcgatttcga cctcgacgct cgaaatattc
       61 tcgccgcctg agatgatgat gtccttggca cggtccttga tttcgacata gccgtcgggg
      121 tgctcgactg cgaggtcgcc cgtgcgcatc cagccgccgc ggaatatatc ggcagtcgcc
      181 ttgggttcct tgagatagcc gagcatcacg gtattgccgc gcaccatgat ctcgccgatg
      241 gtcttgccgt cttgcggcac cggtcgcagg ctctttggat cggccaccat gtggccttcc
      301 accaccggat agcgaacgcc ctggcgcgca gtcagggcgg cccgttcttc gagtggcagt
      361 ttcccccatc catcctgcgg cacgcatagc gtcgacggac cttgcagttc ggtcatgccg
      421 tagatgtgag tgacacggaa gccgagctct tccatgcctt tgatcacctt ggccggggga
      481 ggcgagccgc cggtctggat atcgacgagg tggtcgaacc tgcgccgctg ctccgccggc
      541 gcggaggtga gcaagttgag gacggttggc gcgccgcaca tatgcgtcac gcggtgttcc
      601 gcgatcatcg aaaagatgag tgccgggtca accttgcgca ggcagatatg ggtgccgccc
      661 atcgcgacca ccgaccacgg gaagcaccaa ccgttgcagt ggaacatcgg caaggtccac
      721 aggtagaccg gcttcggggg taacggccag gcgatgatgt ttcccacgca ctccaagaac
      781 gtgccgcgat ggtgatagac cacgcctttc ggattgcccg tggtgccgga ggtgtagttg
      841 agcgcgatcg cgctgctttc atcggctggc gacgtccacg cgaagctcgg atcgcccttt
      901 gcgatgaatt cctcatactc gatcctgccg agccgctctc ccgaccccga gtaaagcgcg
      961 tcgtccacat cgatgacgag cgggcgccgt tcgagcttcg ccaaagcggg accgacctgt
     1021 gctgcgaatt cccggtcggt aatcagcacc ctggcctcac catgctgcaa gatataggcg
     1081 atggttgcag gttccaggcg ggtattgatc gtgttcagca cggcacccag catcggcacg
     1141 ccgtagtgcg cttcgaccat ggcgggcacg ttgggaagca tcgccgaaac agtgtcgccg
     1201 ggacgaatac cggcgcgcgc aagcgccgag gccagtcgcc gcgctcgctc gtagacctgc
     1261 cgataattgt aacgatccgc gccgtggatc atggcgatgc gctcgggatg gatctcggcc
     1321 gatcgcggca ggaacgatat cggcgtgagg gttgcgaaat ttgcggcgtt cggtcgcagg
     1381 gccctgaaga tgtcacgccc gctgccggtg ttgcgccgac tcccgctcag cgacgccttg
     1441 cgcgaccggc ggagaccacc ggtctttgcc ttgcgaagca gctttccggt tggctttggc
     1501 ttggctggtt gggccacatt cttcctccct tagcgcgatt tggctaagtc gctcaacgtg
     1561 ggcttcgata tgtatcataa cagcctgcat gctgcagttc gttgaatttt cttccgcgct
     1621 tgcggatcga agctgccgtt cgaatcggtc tcgcggctcg acaatgcttc gtggcgcaac
     1681 cgatcgaaca tatgttaggc ttacctacca acaagaaatg cgaaaaatcg ctcaacgctt
     1741 caggtggagg atcccatgag agttctgact gccgcatttg cgacgctgct tgcggtccca
     1801 gcgctatttg caagcgaggg cgcggttgcc caggatcgca ctgtcaagat caccggcttc
     1861 ggcgccaagt ccggcgtcgt ccgcgtgttc ggcatcaaca gcgaagcggc gatgaaagcc
     1921 ggcgcggagg agatcaacaa ggccggtggc gtcacgctgg gcgacggaag caaagccaag
     1981 ctgaccgtcg aattcctcga cgaccgttgc aacgccgagg aaggcatctc ggttgtgcgc
     2041 cgcattgcct cgaccgacgc tttcgttgcg gtcgggccga cctgctccaa cgtggccgaa
     2101 tcgcttttcg ggatcctgca gaagaaagtg ggtgatgcgt ccgatagcgg cctgcaattc
     2161 cccatcatcg ccgacgtggc cgctaagggc ggacttgcca aaatctccga gtggtcgttc
     2221 cggaacgtgc cgagcgaatt cgagatgtac aagtcgctat tcgcgtggat caagtcgcag
     2281 catccggacc tgaagaccat ctttggcggc gtcgagaaag atttcgcaca ttcgaatgca
     2341 acctacggcg tcatgaaggc acaagccgac gccaacggct tccagctgct cggtacatcc
     2401 gaatggctgc tcaacgacac caccttctcg acccaggtcc gcgacatgaa gcgcgccaat
     2461 gccgatatcg tcgcgatatc ggcgcatccc ttcacaacct gcggcgtgct caaggagatg
     2521 gaacgtcaag gcgtgaagcc caagctcctg atcggcctca ccagctcgtc cagcatcgaa
     2581 acgctgcagg gctgcgccaa gcaagcggaa ggcatcatca ttccgacgag tttcgcgccg
     2641 gtgacgccgg aggccaagaa agcggccgag aacgtcgcca agttcggtgg aagcgccgat
     2701 ctgcacagcg ccgcggcgta cgagatcatg ttcatcctca aggatgtgat cgagcagcag
     2761 aagatcttgg cgaagcccga caccgtgcag gccgaccgca acaagatgcg cgaaggccta
     2821 gcggcgttga aagagacgaa cggcctcttg ggcaaggtcg gacgcaccgc tgatcgtgaa
     2881 gcaatcaagc cgttcctcta cgtccatgcc aaggacggaa gctggctggt cctgcacaag
     2941 ccatcgtcgt gaagcggccc gagcatttcg gcgggccgca ccggcggtcc gccgccttgt
     3001 tgtcaatcag tcgccttgcc gtcaatcatt catcggtact gaccaattgc ggccattgac
     3061 gtggacgtgc tcgacctgat cgacgctgtg cagtcggttg ccgatggcgt gctgttcggc
     3121 gcgacctatg cattgctggg aattggtttc acgctcgttt tcggcgtgat gcacaagatc
     3181 aacttgtcct atgccgcagc ctcgatcgga gccgcttacg cgagccttct gatcgtcgcc
     3241 ggcatcaagg cgcccgcgat cctcgtattc ctggcgtcgg cggcggcagg cggagtcatc
     3301 ggcggcctcg tttatctcgt cggcttcaag ttcattccgc ttgccaaccc gctggcaacc
     3361 ctgatgtcca ctgtcggatt gctgctcctg atcgatgaaa tcatcgtgca cacgacggcc
     3421 ggcgttccgc tcaattatcc ggcgctgttt tccgacgtga tcgtttcggc agggccgttc
     3481 aatctgcgcg gcgatctgat gttcgtcttc gctctgagct gtgcgtgcat ggttgtcctg
     3541 ctgctgttgc tttatcgaac caggctcggc ctcgctactc gcgccgtgtc gcagcagccc
     3601 acggcatcgc agctctgcgg catcagcatc acgcgggtga acgttgtcac attcgtcatt
     3661 accggcgtgg tcggcggcat tgccggagcc atgacagggg cggccatcgg catgctgtcg
     3721 ccgctgcttt cgctgccttt gaccgtcaag gggctgatcg tcaccgtgat cggagggctc
     3781 gggagcatcc cgggcgccat catcgccggt ctcgtggtcg gcggtcttga aaacgctttt
     3841 cagttcttcc gcggcgtatc ggagcgcgat ctctacatca tgctgttgct gttcgctttc
     3901 ctcattttcc ggccgggcgg catgtttgcg atgccggttg ggcgggattg aaggcggggc
     3961 acaacccgat ggatttttac ctcggactta tgcagatcgt cggcgttcat acgctgctcg
     4021 gactgtccgc ctattgcgtg ctgctcaccg gccaggtcag tctcgcacag gccggcttct
     4081 tcgcgatcgg cgcgtacgtc gccggcatgc ttacggtgct cgcgcaatgg ccgcttgtcc
     4141 ccgccctcgg cgcgtctgcc gtggtcgccg gcgtgatggc ttgcgccgtc gggttccccg
     4201 ccttgcgggt gaagggcctg atgctcgtgg tggcgaccat cgcattcgga gaggccgtgc
     4261 gtctgttctt cttcaatttc acctatcaag tgcaggccgg aaatctcaaa ctcggtcccc
     4321 atggcgccga gggcttccgc cagatccgct actttcccga gcatggctgg acgacgcttg
     4381 acgtgatgct cttcatctgg gcggtggtcg ccctggtgat ggccggcgta tggtggttgg
     4441 atcgatcgcg cgccggcgcc gtattgcgtg cggtcggcga ggatgagctc gccgcacaaa
     4501 gcgccggcat caacattact gccgtaaagg tctccgcgat gacgatcggc ggcgtcatcg
     4561 ctggcatcgg cggcggcatt ttcgcgcatt acacgacgca tatcgagcat ctgcagttcg
     4621 gcgtcgtgct ggcgaccttc gccatcgcct atccgattct cggcggtctt tccaatgtgt
     4681 tcggaaccct gctcgccgtc gtcttcgtcc agggcttcct gatcgaaggt cttcgcttca
     4741 tgggcgattg gcgcaatctg ctgttcggcg ggttgatcgt gctcgcgatg aacgtgcgcc
     4801 cgcgcggatt gctcgacgcc ggggccgtgc ttgccctccg gcggctgatc gtcccgagga
     4861 aggatcgcaa cggtgcttga tctgcagggg ctcaccaagc attttggcgg cgtccgcgcg
     4921 gtcgacggca tcgatctggc cgttacgcgc ggggaaatcg ttggcttgat cggcccgaac
     4981 ggctcgggca agagcacgat cgtcaatctc atatgcggtt tgcttccccc caccgcaggc
     5041 cgcgtcgtct tcaacggtgc cgacatctct cggctgccgc cgcatgcgcg tgtgacgctt
     5101 ggaattgcac gcacgttcca gaatatacgc ctgtttggac aattgacggt ttggcagaat
     5161 ctttgggtcg cgcagaattc cggcgagcag cgcaaccaga attttctccg ccgctggttt
     5221 ggcggctcga acggcgcatg ggaggagatc aaccggacgc tcgagttttt cgatcttgcg
     5281 cacaagcgtg atgaattggc agccaatctg gcattcggcg agcagcggcg gctcgaattc
     5341 gcccgtgctc ttgctgcgaa gcccgtcttg ctgctgctcg acgagccggc agcgggtatg
     5401 aacgccgagg aagtcgatca actcgacagc cgaatccgca agctgcgcca ggatggcatc
     5461 accgtgctct tgatcgagca ccatgtagaa ttggtcatgt cggtggcaga ccgcgtcgcc
     5521 gtgctcaatt tcggccagaa gatcgcggaa ggaacgcctg ctttcgttca ggcggacccc
     5581 gcggtgcgcg aggcctatct cggcagcgcc gcggtcgcat gaggactgag atgctcgaca
     5641 tatccgacct cgtcaccgcc tacggtaaga tcgacgcgct gaaaggcgtg tcgctgcagg
     5701 tcgagaccgg aaaaatcacc tgcctgctcg gccccaacgg tgcgggcaag accacgttga
     5761 tgatgacaat tgccggtctc ttacatccgc gccgcggctc gatccggctc gccggcaccg
     5821 aactggttgg actctcgccg gccagaatcg tcgcgcatgg catcgcgctc gtgcccgaga
     5881 accggctcgt cttcccgcag atgagcgtgc gcgaaaatct gctcgcgggc gcttatcagc
     5941 gcaacgacaa ggtcagcatc gatgccgatc tcaagcggat gtatgccctc tttccacgtt
     6001 tgaacgagcg gaccgagcaa ttggccggca ctctctccgg cggggagcag cagatgctcg
     6061 cggtcgcccg cgcgctcatg tcgcggccgc gcgtgctgct catggatgag ccgtcgctgg
     6121 ggctcgcgcc ccttgtcgtc acggagatat tcaatatcgt ccgcgacctc aatcgcgacg
     6181 gaaccaccat cttcctggtc gagcagaacg cgcacatggc cctgcaggtg gctcatcgtt
     6241 tcttgctgat ggagcagggg cgcatcacgt tcaggggaat gcccggccag ctggccgaag
     6301 atgaggtcgt ccaacgcgct tatttgggga cgcgccgcgc ggccggctga cgcaaccgcg
     6361 gtagaggccg atgatcgacc tcgtccagaa caccctcgac ggcgtgatga tcggctcgtc
     6421 ctacgggctg ctggcgctcg gcttcaccct gattttcggc gtgatgcgca ggctcgactt
     6481 ggcctatggc ccgtccatca tgattggtgg ctatttcgga accgtcctct atttgtacta
     6541 cggggccggg ccgctcgcgg ttgcgagcgc cgtggttctc ggggcggtga tcgccggaat
     6601 ctatgtcgag cggctctgtt tcgcgccgat gaaagccggc gccgccatcg catcaatggt
     6661 gtccagtttc gcgatctgga tgcagctcga gcaggcggcg atcctggtct tgccccgcca
     6721 tacttatccc tttccaccgc tcgcaaccgt cgcgccgctc gaattcgggc ccttcctggt
     6781 acgagtcgat cacctcatca tgctggtctg cgcgctcgca ctggcggcgg ccgtgcatct
     6841 cgcgctctat cacacgcgtt tcggccttgc cttgcgcgcc atcattggca gcccggtcgc
     6901 ggctcatctg gtcgggatca atgtgcggac ggccgtgctg ctgacattcg cgggcgcctc
     6961 ggcggtcggc ggcgctgccg gcttcctcgt gcttgcggtc gaccagcagg tcacgccgat
     7021 gttcggtatg tgggcgacat tgaagggcct gatcgccatg atgatcgggg gactcggatc
     7081 cgtccccggc gcggttgtcg gcggattgct gctgggcatc gtggaggtta acagccaatg
     7141 gtacttcgga ccgcagattc gcgatctcgt cgcttatgtc gtgctgtttg catttctggt
     7201 ggtgcgtccg cggggattgc tcggccgcgc gcccgaaccc gcgatgccgc cgaaggcttg
     7261 atccatggac gactatctca tcggggtatt gtccaatatc ggcgtcatct cgttcgtcgc
     7321 gctgtccgcc tatctgctgc tgctgaccgg agagatttct ttcggccagc aagccttctt
     7381 cgcgatcggg gcatatgccg cgggaatagc aaccgccttg tggggctggt cgctggcagc
     7441 ggccctcgtt tggggcgcag cggtcagcgg tggcgcagcg ctgctggtcg gtgtgccgac
     7501 gctgcggctg cacggcctct acttcgcgat cgcgaccttg gcgttcgcgg agatggtgcg
     7561 gattctcttc gagctgttcc gttatcagat ttcgatcgac ggcgagttga tcgggcccaa
     7621 cgggaccgac ggatttcgca acattcgtta catcttcgac cacaacatcg acgcctttca
     7681 gttcctgctg ctgatctatt cattgctcgc ggcggcgatg ttgggattgc tgctgctgga
     7741 acgctcgcgg ctcggtgtca tcttccggat gatcggccag gatgaaacac tcgccgaatt
     7801 gcagggaatt cccgtcgcgc gccgcaaggt gctcgccgct gggatcgctg ggacgctcgc
     7861 cggaattggc ggcgggctct atgcccacct gacgacctat gtggagccac gcattttcga
     7921 tatcatgctc ggcgttcaca gcctcgccta cggcttgatc ggcgggctgg ggaccgcatt
     7981 cggtccgctg cttggcgttc tgatcgacat cggcctgctg gaatccataa gagcattggc
     8041 cgggtatcgc atgatcatct tcggcgggct cgttgccttg ctgttgatcg tgcgcccgcg
     8101 cggtttgctc gacgagatgg tggtgcacaa gatcggccgt ggctggcggc gagtttccct
     8161 atgggcacgg cggtcaagcc tgaaaccgag gggaaacagc gcctgagcga ctggaggatg
     8221 gccgtgaagt cggcaactta cgtcttagcc acgctcgcga tgcttgcgcc ttgcggtctc
     8281 gaaaacgcgc atgcgcagat ccgggccaag gccgacgtta gctgccagcc ggccgagaag
     8341 ctccaatacg actgcattat caagctcgcc aatgcccgca cgagcgaacc tttgtcgggc
     8401 ctcacactga cggtgggagc ggacatgcct tcgatgccgg cggcccataa tatgcggccg
     8461 gtgaaggcga ccgaggagca ggacaagggc acgtatcggg cgcgcattgc gctcgaaatg
     8521 tacggcgact gggcgcttcg gctggaactg tccggcacgg tacgcgatcg cgtggtcaag
     8581 ctgctgcgat tcgagagcga tcgcgtgggc gaggcgacgc cggcgcccgg gcgtcatcga
     8641 cactagctat actgaccgcg ggaatagcag gcgactaggt ggcgccgtcg ctcggggggt
     8701 cgagctgcgc ccgatccata cgcgcgacgc cagcgagatc gagcgcgccg tcgttgcatt
     8761 cgcccgcgag ccgaacggcg gcctgatcgt gacggcaagc gcgtcgggcg ctcattcatc
     8821 gcaatcagat cattgcacta gccacgcggc tccgcctgcc gaacgtctat ccgttccgct
     8881 actacccttc gcacggtggc ctcgcctcct acgggcctga tcaaatcgac cagtacaagc
     8941 gcgcggccgg ctatgtggat cgtatcctca agggcgagaa gccaagcgac ctgccggtgc
     9001 aaacaccgac caagttcgaa ctggtcatca acctcaagac cgcgaaggcg atcggcttcg
     9061 aactgccgcc gacgctgctc gcgcgcgccg acgaggtgat cgaatgaatc gtcgcgagat
     9121 cattatgctc attggcggcg cggcggcgtg gccgctcgcg gcgcgtgcgc agcagggcga
     9181 gcgggtgcgg cgcatcgacg tgctcatgag cgcggctgcg gccgatttgg aaggacaggc
     9241 ttgcatcgct gcgttccgca gggctggctc cgatagcatc ctttgatgag tggacggaat
     9301 ggcgcgctca gacgcgcgga ggtgcagtga tgcggtctgc aatttctg
//
LOCUS       DATLJT010001134         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_768638,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001134 DATLJT010000000
VERSION     DATLJT010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768638"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VM910_26170"
     CDS             complement(<1..692)
                     /locus_tag="VM910_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase"
                     /protein_id="HVI66043.1"
                     /translation="MRLPFRFGVVTLTEAPQVFVRARIELPNGSSHWGAAAELLVPKW
                     FDKNLALSNEENFEQLRLALVLATDAYTEPAGPRTAFGHFAAHYDQQIAAGAARGLNP
                     LVASFGPAQIDRAVLDALCRSASCSFYQAIGANLPGIDPGLLSTQLIDLAGFDMRRFL
                     ARLTPATTIAARHTVGLVDAIAGHPRKINDGLPESLEEVVAVYGHTFFKLKVSGSADA
                     DLQRLIEIAAVL"
     gene            1037..2164
                     /locus_tag="VM910_26175"
     CDS             1037..2164
                     /locus_tag="VM910_26175"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI66044.1"
                     /translation="MSRIVRFEVGRFDYDLAGEFKFFKTPVRPSIVVRLTDENGVQGW
                     GQSVPVETWTYETSESVETTLRHYLAPAILGADPSDLAAIHERMDRAIRPSFSVGQPV
                     CKAAVDLACYDLWGKQTKRSVSEILGGASKKQVKLSWTVQSPTMTGAEMQLAVAKTRG
                     YDSFNIKLGYPQTPEYDLHLVRTVCNFAPNSFHWADANTNYDVETALAMAPKLADAGL
                     KGLESPLPPNLIRGYQRLKRQGALPILMDEGIISPVEAAEFIALEMFDGIAMKVARCG
                     GLWNASRIVSLLRENNLLMLASGLTDPDLSLAASVHLFAWAGLSTPAALNGPQYISAG
                     GTTDPTFRANGDIIRVPVAPGLGITLDERAEKSMTIAAEAG"
     gene            2226..>2543
                     /locus_tag="VM910_26180"
     CDS             2226..>2543
                     /locus_tag="VM910_26180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66045.1"
                     /translation="MTMAQQKLQTDRSGRIGVKLMRLVTPVAGVLVFLAIWQAGVMLS
                     RVPAYLLPAPTEIAHTFIDEFPRLLRHGWFTTYEMLLGYLLAVAIAIPLAIAITSSQR
                     FDEF"
BASE COUNT          529 a          755 c          775 g          484 t
ORIGIN      
        1 tccagcaccg ccgcaatttc gatgaggcgc tgcaggtcgg cgtcggccga accgctgacc
       61 ttgagcttga aaaaagtgtg gccgtagacg gcaacgacct cttccagcga ttccggcaat
      121 ccgtcgttga ttttgcgggg atggccggca atggcgtcga ccaggccgac cgtgtggcgt
      181 gcagcgattg tcgtagcagg cgtcagccgc gcgaggaatc gccgcatgtc gaagccggcg
      241 agatcgatca gctgcgtcga cagcaggccg ggatcgatgc cgggcaaatt agcccctatc
      301 gcttgataga acgaacagct cgcgctccgg catagggcgt cgaggaccgc acggtcgatc
      361 tgggccggac cgaagctcgc gaccagcgga ttgagaccgc gcgcagcccc cgcggcaatc
      421 tgctggtcgt aatgggcggc gaaatggcca aatgcggtgc gcggtcccgc cggttccgta
      481 taggcgtcgg tggcaagcac gagcgcgaga cgcagctgct cgaaattctc ctcgttggag
      541 agcgcgagat tcttgtcgaa ccacttgggc acgagcagtt ccgccgctgc tccccagtgc
      601 gagctaccgt tgggaagttc gatccgcgcg cgcacgaata cctgcggcgc ctcggtgagc
      661 gtgaccacgc caaaccgaaa gggcaggcgc aagcgtacct ccctctcgta gaggcacact
      721 tctcgaaccg tgaatacggg cgccgtcacg tcagtgattc ggaatggccg cttgaggcac
      781 tgtaggcgaa aaggccatca gcacaagccg ggtaaacagc ttgaaacttg tgatcatttg
      841 cccccgttct gttgcgccgg cgaacggtcg tcgaattcta aggcgatcat cgtgccgccc
      901 tttcgttcgc acccctttga ccgcatgatg aagataaagc gaatttgacg ttgcgtcatg
      961 tccgacaaac atagccttgg cgcccaagac cttggcggcc aacaaacgtg cggactgagg
     1021 gagggctaca actgcaatga gccggatagt tcgctttgaa gtcggcaggt tcgactacga
     1081 tctggccggc gaattcaagt tcttcaagac gccggtgcgg ccatcgatag tcgtgcgatt
     1141 gaccgacgaa aacggcgttc agggttgggg gcagtcggta ccggtcgaaa cctggacata
     1201 cgagacctcg gagagtgtcg agaccacgct gcgacactat ctcgcgcccg ccattctggg
     1261 cgcagacccg tcagatcttg cggcaattca cgaacggatg gatcgtgcga tcaggccatc
     1321 gttttccgtt ggccagccgg tgtgcaaagc ggccgtggac ctggcgtgct acgatctgtg
     1381 gggcaagcag accaaacgca gcgtgtccga gattctcggc ggtgcaagca aaaaacaggt
     1441 gaagctcagc tggaccgttc aatcgccgac gatgacaggt gcagagatgc aactcgcggt
     1501 cgccaagacg cggggctatg acagtttcaa tatcaagctc ggctacccgc agacgccgga
     1561 atacgatctc catcttgtcc gcacggtgtg caacttcgcc cccaatagtt ttcactgggc
     1621 ggacgcgaac accaactatg acgtggaaac cgcgctcgcg atggcaccca agctcgcaga
     1681 tgccggcctc aagggcctgg agtcgccgct tcccccgaac ctaatacgtg gctatcagcg
     1741 cctgaagcgg cagggcgcgc tgcccattct gatggatgag ggtattattt cgccggtgga
     1801 agctgcggaa tttatcgcgc tcgaaatgtt tgacggaatc gccatgaagg tggcgcgttg
     1861 cggaggactt tggaacgcgt cgaggatcgt gagcttgctg cgggaaaaca acttgctcat
     1921 gctcgcctcg ggactgaccg accccgattt gtcgctggcg gcatccgtcc accttttcgc
     1981 atgggcagga ctgagcaccc ccgccgcgct caacggcccg cagtacatct cggcgggagg
     2041 cacgaccgac ccaaccttcc gcgccaacgg cgacatcatc cgcgtcccgg tagcgccggg
     2101 gcttggcata acgctcgatg agcgtgcgga aaaatcgatg accattgcag cggaagctgg
     2161 atgatcgtgc ctcagtggca ccgcacactc cgataggtta cccgatcgct cgaccgcact
     2221 caggtatgac catggctcag caaaaattac aaacggacag aagcggaaga atcggcgtca
     2281 agctcatgag attggtgacc ccggttgctg gcgttctcgt ctttcttgcg atctggcagg
     2341 ccggggtgat gctctccaga gtgcccgcct atctgttgcc cgcgccgact gaaatcgccc
     2401 acacattcat cgatgagttt ccaaggctgt tgcgacacgg atggttcacg acctatgaaa
     2461 tgttgctggg atacctcctg gctgtcgcga tcgccattcc gctggcaatt gcgattacct
     2521 catcgcagcg cttcgacgaa ttc
//
LOCUS       DATLJT010001135         5208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1460677,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001135 DATLJT010000000
VERSION     DATLJT010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460677"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1307)
                     /locus_tag="VM910_26185"
     CDS             complement(321..1307)
                     /locus_tag="VM910_26185"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66046.1"
                     /translation="MKRRAFIALLGGAAAWPLAARAQKTERMRRIGVLLPAAADDAVF
                     QARMGAFLQELALLGWGIGRNVRIDIRWGTTDAAEIRRQAAELVALAPDDILAGGIST
                     VPSLMQATRTIPIVFVMVSDPVAAGFVDSLARPGGNVTGFMNYEYSMSGKWLELLKEV
                     APTVTRVAVLRDPTISSGIGEFAAIQAVAPQLGIDVSPMSVRDAAEIEHAVTAFAGIP
                     NSGLIVTAGRLAVANRELILMLAARHKLPAVYFERFFVATGGLISYGPNYIDQFRRSA
                     GYVDRILKGEKPADLPVQNPTKYELVVNLKTAKALGLDVPWILQQRADEVIE"
     gene            complement(1304..1498)
                     /locus_tag="VM910_26190"
     CDS             complement(1304..1498)
                     /locus_tag="VM910_26190"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66047.1"
                     /translation="MALIVDQYRLAAGYVDRILKGEKPGDLPVQAPTKYLLAINLKTA
                     NALGLTVPPTLLARAHEVIE"
     gene            complement(1452..2345)
                     /locus_tag="VM910_26195"
     CDS             complement(1452..2345)
                     /locus_tag="VM910_26195"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66048.1"
                     /translation="MANASARERAFCTRGAAGVGRREFIKLVGGAAAAWPLAARAQQA
                     ERMRRIAVLMPFTADDPEGQARLLAFAQGLQQLGWTVGTNLRIETRWGAGDDERNRKY
                     AAELLALAPDVALANGSPEVSALRQAARSVPIVFVNLPDPVGQGFVATLPRPGGNVTG
                     FTPLDEFGQSGKWLELLKEIAPQVTRAAVLLNPAFATGIAQLGAIQAVAPLFGVEVTP
                     IDLRDAAEIEHAVTEFARTPNGGLIVTPTGSGARRELIISLAARLRLPAVYPYPFYVA
                     SGGLTSYGPDCRPVPARRRLR"
     gene            complement(2549..3145)
                     /locus_tag="VM910_26200"
     CDS             complement(2549..3145)
                     /locus_tag="VM910_26200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66049.1"
                     /translation="MTPVIFADIVGFGKITARMKAYEIVACLNQLFSEFDRLAEDAGI
                     EKIKTIGDNYMAVCGVPTPRPNHARMAAKFALDMVAATARLRPRLPVPFPIRVGLHSG
                     PVMAGVIGARKLAYEVWGDTVNIAARMEAASAPNRVLASAATVKGLGSDYSFDGPHKI
                     DTKEGRVVEAFFVSYQPLRSAQIGGPLSGQTGKHLLRF"
     gene            complement(3078..3662)
                     /locus_tag="VM910_26205"
     CDS             complement(3078..3662)
                     /locus_tag="VM910_26205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66050.1"
                     /translation="MDLPGILIVDDNEDDRYTLQLLLESDGHERIASAASGNEAIALI
                     EKEKFSLVLLDLMMPDLNGDEVLKVIKSDPDKRDIPVVMISAETDADKVSQCIELGAD
                     DYLPKPFNPTILRARIAAALRRHSLQALENEYLGKIENEKRHSENLLRNILPAEIATR
                     LCNGESNIADYFDDACHFCRHCGLRQDHGAHEGL"
     gene            3973..4095
                     /locus_tag="VM910_26210"
     CDS             3973..4095
                     /locus_tag="VM910_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66051.1"
                     /translation="MSTSTATYRGRMYLQDAADDYAIGKHVEVIVIPLAGRPRS"
     gene            complement(4138..4395)
                     /locus_tag="VM910_26215"
     CDS             complement(4138..4395)
                     /locus_tag="VM910_26215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66052.1"
                     /translation="MLLRPEYLSQKLDQPLPTKDGGTLRTIRDACDYMTGMGKKRELR
                     DHWRRACKLILAKADVQTVSRVLELALFMDAKLDVSKVPAK"
     gene            5021..>5208
                     /locus_tag="VM910_26220"
     CDS             5021..>5208
                     /locus_tag="VM910_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66053.1"
                     /translation="MAAVTAIAALLFPTLLALFVSPDWKRLGFERYFEMQAGNFVALA
                     AYYMVICGVFAFGAHLLRR"
BASE COUNT          981 a         1582 c         1564 g         1081 t
ORIGIN      
        1 cgccctgctg cgcccacgcg gcgagcgacc atccggttac accgctgccg agaagtgcga
       61 tgaattcccg ccgcctcatg ggcttacccc aaggccaagg atcgcggacc aagtatagca
      121 ggtcttagag ctggttcggg aagatgcatc gcagcaaaga cgggccgctc atgtccgctt
      181 tgggtcattt gcgtcagcgg agaggctgcc gccacacgtc gttatgtccg cttcgccccg
      241 aaagcggaca aatagcagat cgtttcggta tgtccgcttt gtgccaatag cggactcatg
      301 caccgcaaca aaaagtaccg ttattcgatc acttcgtcgg cgcgctgctg aagaatccaa
      361 ggcacgtcga ggccgagcgc ctttgcggtc ttgaggttga ccaccaattc gtatttggtc
      421 gggttctgaa ccgggagatc ggccggcttc tcgcccttga ggatgcggtc gacatagccg
      481 gcggagcggc ggaactggtc gatgtagtta ggcccatagg agatcaagcc accggtagcg
      541 acgaagaagc gttcaaaata gaccgccggc agcttgtgtc gggctgccag catgaggatc
      601 agttcacgat tagccaccgc caaccggccc gctgtaacga taaggccgct attcgggata
      661 cccgcgaagg cggtgacggc gtgctcgatc tcagcggcat cgcggacgct catggggctc
      721 acgtctatac cgagttgagg cgcgacggct tggatggcag cgaattcgcc gatcccggag
      781 gatatggtgg ggtcgcgaag gactgctacc cgcgttacgg ttggcgcgac ttctttgagc
      841 agttcgagcc atttcccgct catgctgtat tcataattca tgaagccggt gacgttgccg
      901 ccaggccgcg ccagactatc aacgaagccg gcggcaaccg gatcggagac cattacgaat
      961 acgatcggta ttgtgcgggt cgcctgcatc aacgatggca cggtcgagat gccaccggcc
     1021 aggatgtcgt cgggcgcgag cgcgaccaat tccgcggcct gtctgcgaat ctcggcggca
     1081 tcggttgtgc cccagcggat gtcgatgcgc acgttgcggc cgatgcccca gcccaatagc
     1141 gccagctcct gtaagaaggc ccccatgcgg gcctgaaata cggcatcgtc cgcagctgcg
     1201 ggcagcagca caccgatacg ccgcatccgt tcggtcttct gcgcgcgagc cgcgagcggc
     1261 cacgccgccg cgccgccgag tagtgcgatg aaggcgcgtc gcttcattcg atcacctcgt
     1321 gggcacgggc gagcagcgtt ggcggcacgg tcaggccgag cgcgttggcg gttttcagat
     1381 tgatggccag caggtacttg gttggcgcct gcacgggaag gtcgcccggc ttctcgcctt
     1441 tgagaatgcg atcaacgtag ccggcggcga gccggtactg gtcgacaatc agggccatag
     1501 gaggtcagac ctccgctggc gacgtaaaac gggtaggggt agaccgccgg caatcggagt
     1561 cgggctgcaa gagaaatgat gagttcgcgc cgcgcgcccg atccggtcgg ggtcacgatc
     1621 aggccgccgt tcggcgtgcg cgcaaattcg gtgacagcgt gctcgatttc ggcggcatcg
     1681 cgcaggtcga tcggagttac ctccacgccg aacaacggcg ccacggcttg gatggcgccc
     1741 aactgtgcga tccccgtggc aaacgcggga ttgagcagga ccgccgcgcg cgtcacctga
     1801 ggggcaatct ccttgagcag ctccagccac tttccgctct ggccaaactc gtcgagtggc
     1861 gtaaatccag tcacgttgcc ccctggccgc gggagggtcg caacgaaacc ttggcccacc
     1921 ggatcgggca ggttcacgaa cacgatgggc acgctacgcg ccgcctgtcg caatgcgctc
     1981 acttccgggc tgccgttggc aagggctacg tctggggcaa gcgcgagcaa ttctgccgcg
     2041 tatttgcgat tgcgctcgtc atcgcccgcg ccccagcgcg tctcgatgcg caggttggtg
     2101 ccgaccgtcc agcccaattg ctgtaggccc tgcgcgaacg ccaggaggcg ggcttgtcct
     2161 tccggatcgt ctgcggtgaa gggcatcagc acggcgatgc gccgcatgcg ctccgcctgc
     2221 tgcgcccgcg ccgcgagcgg ccacgccgcc gcagcgccgc cgacaagttt gatgaactcg
     2281 cgccggccga cgcccgcagc tccgcgcgtg caaaaagctc gctctctagc ggatgcgttc
     2341 gccatgttgt taccatggcc ccatacaagt aggcagaacc ctctcgaatt gcgccttgat
     2401 ctaaatcaat tgtgacgatc gcttcgacga ccgccgaccc acatagagga tcggggcact
     2461 ggcgacaccc ctcatgcgtt cgaccgaagg acaccggcag gaaactgctg cgctgcggga
     2521 tttccattcg gcctatgtcg ggttggggtc aaaagcggag caaatgtttt cctgtttgcc
     2581 ccgaaagcgg acctccgatc tgtgcgctac gtaacggttg ataactcacg aaaaacgcct
     2641 ccaccacgcg gccttccttg gtgtcgatct tgtgcgggcc gtcgaagctg taatcgcttc
     2701 ccagcccttt gacggtcgcc gccgacgcga gcacgcgatt cggcgcactc gccgcctcca
     2761 tgcgcgcggc gatgttcacg gtgtcgcccc agacctcgta ggcgagcttg cgcgcaccga
     2821 tgacccctgc catcacgggg ccagaatgca ggccgactcg gatcggaaac gggaccggca
     2881 gccgtggccg caatcgcgcg gtggcggcga ccatgtcgag agcgaatttc gccgccatcc
     2941 gggcgtgatt gggacgcggc gtcggcacgc cgcaaaccgc catatagttg tcgccgatcg
     3001 tctttatctt ttcgatccca gcatcttcag caagcctgtc aaattcgctg aataattgat
     3061 tcaggcaggc gacgatttca taggccttca tgcgcgccgt gatcttgccg aagcccacaa
     3121 tgtcggcaaa aatgacaggc gtcatcgaag tagtctgcga tgttgctttc cccgttgcac
     3181 aggcgtgtcg cgatctcagc cggcagaatg ttgcgcaaga ggttctcgga atgccgcttc
     3241 tcgttctcaa tcttgcctag atactcgttc tccagcgctt gtagcgagtg cctgcgcagc
     3301 gcggcggcga tccgcgcgcg caggatcgtc ggattgaacg gcttcggcag atagtcgtcg
     3361 gcgccgagct cgatgcattg cgaaacctta tcggcgtccg tctcggcgct gatcatgacg
     3421 accgggatat ctcttttgtc cggatcgctc ttgattactt tgagaacctc gtccccgttc
     3481 agatccggca tcatcaggtc gagcagaacc agactgaatt tttccttctc gatgagcgca
     3541 atcgcctcgt ttccgctggc cgcgctggcg atccgctcgt gaccgtcgga ttccagtagc
     3601 aattgcagcg tgtatcgatc gtcctcattg tcatcgacga taaggatacc tggaaggtcc
     3661 attgccatag cctgagccct ggcgcagatc gacgcatcgc cggtcagaat ctgtccgcta
     3721 aaacgcagca ctgacttgat tcagatcaag gatgactgac ggcattgtgc gtcacagcaa
     3781 ttttggctac cgacagtccg ctatgggtca taaccagaca aactcggtct gagattagat
     3841 gtccgctttg cccccgaaag cggacatggc cttacgcccg cctccagctt ttagcgaatg
     3901 ccgacattgc agcctcgcgc gtcgccgcgt agccgtgcgt cggggtgcgg tcctcatgct
     3961 gcccgaaggc tagtgtccac atccacggcg acctaccggg gccgcatgta ccttcaagat
     4021 gcggccgacg actacgccat tggcaagcac gtcgaagtca tcgtcattcc actggccgga
     4081 cggccgcgaa gctgaggcgc gtctaagaat caggtagtcc ttgtccatgt gccgcgctca
     4141 tttcgctggc actttcgata cgtctagctt ggcatccatg aatagcgcca actcaagcac
     4201 cctgctaacg gtctgcacat cggcctttgc aagaatcaac ttacaagccc gccgccagtg
     4261 gtcccgtagc tcccgcttct tgcccatgcc ggtcatgtaa tcgcacgcat cgcggatggt
     4321 gcgcagcgtg ccgccgtcct ttgtcgggag tggctggtct agtttttggg acaggtactc
     4381 aggacgcaac aacatgggct ataagatgga gccggggcgc gggcgagtcg agtctgataa
     4441 aagatccttt atcgtttcca tcaaaataat gatcattcta agtgtgaagt ggctcaccca
     4501 tttctcccga tttgcccctc taaaagtccg gtactggagc caggagggaa acatggcacc
     4561 gaaaccaaaa tcaaaactag ctaggttcgt atttgccgtc gcgatggcat tgaccatcct
     4621 agcggtcatg acgtgggaga tggctgcgcc gagctcgcta atttatccga tttaccagcc
     4681 tcaacttttg cttttgtact gagggcaccg acatgcgcga gcatgtgctc ctattctgtc
     4741 tgttgggtct ggtgctcata gccacgatca tactggtgac cgcaccgggg accgtgattt
     4801 gggcgctggc cctcatcgaa tgagctgccc ccacctcatt cgcgtggtct tgctctggat
     4861 tgtcgtctga gctaccgcca gcgcttagcc ccgcgaccag cggggctttc tttgaggtga
     4921 gccgcgggga ctaaaactaa tgtggaccgt tagagacgcc gaacacattg tgattcttat
     4981 ttcaatcgcc ggcggattgt tttgcgcgcg ggtctggtca gtggcggcgg ttacagcgat
     5041 tgccgctctg ttgtttccga cgctgctggc gctgttcgtg tcgcccgact ggaaacgcct
     5101 tggcttcgag cgctatttcg agatgcaggc aggcaatttt gtagcactgg ccgcctatta
     5161 catggtgatc tgcggggtat ttgcttttgg cgcgcattta ctccggcg
//
LOCUS       DATLJT010001136         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1920222,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001136 DATLJT010000000
VERSION     DATLJT010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920222"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(684..1664)
                     /locus_tag="VM910_26225"
     CDS             complement(684..1664)
                     /locus_tag="VM910_26225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66054.1"
                     /translation="MTSPRRQFLRLAAGAAALAAVPRIGRAQAYPTRPVRVIVPFAPG
                     GPTDVFARLMAQKLSEQMGVQFYVENIAGAGGNIGAGRAAQSSPDGYTLLVNGANHVV
                     NPALYQRVPYDPTKDFDPVTLAVTAPAVLTVNPALPVDTVRDLVALVKAHPGKYSYAS
                     PGVGTPPHLVGELFRLSLGLDLIHVPFNGGGPAIASAVAGHTPISFGSTAPAVPLVKD
                     GKLRAFAVSTKTRSQALPDVPTMTEAGYPDVAGESWFAVVVPAGTPKDIIALLQREIA
                     QAISRPDMQERLATLGYEPVASTPEDCASRFRTEIAKWGKVIGEAGIKAQ"
     gene            2011..>2406
                     /locus_tag="VM910_26230"
     CDS             2011..>2406
                     /locus_tag="VM910_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66055.1"
                     /translation="MVDVSQFLGLRAAKRTGPSSPAAGQLGIFASESGPIPQRAVRFS
                     AWQIAALFMTLAAIASIPILLYPWPPLSDYINHLARMHVIATIGSDPNLALFYQVEWQ
                     VIPNLMMDLIVPILQRVMNVYLAGQVYTIM"
BASE COUNT          429 a          759 c          761 g          457 t
ORIGIN      
        1 ctcttgcgct tgcggacttc ggctcgaagc caggtccggt agcgatcaac gcaggtctgc
       61 gaacagaagc tcttgaacgc tcgacgatgc cgtaccaggc cgaacttgcc atggcagtag
      121 gcgcaatgat ttttcatagc tccctcccga atggcatctc ggttgaaccg accggctgag
      181 gatcaccgtc cttagtgcgc tgcagcatgg cgcgtcgcaa tgcacaatta gtattacacc
      241 tcaaccattg agaattcgtt ttgccggccc gccatacgcc caaaacattg aatccatatc
      301 ggtttttcgg ctcaacccgc catggcgccg gcacgccgcg tggcggcgct atttcgggtt
      361 tgtcgtgccg caatgagggg cgtcgttcct gggcgatccg ctgttcaggg gagcgctccc
      421 gggcacgaca accttcaagg agcgagcgca gcggcacatt gcagcactcg cgcgaaattg
      481 gctcatcgag acggttgcga tcctcgccgc gctcgcaggc gccatcattt catttctgca
      541 gctttttcgt ttgcttcgtg agtgagacgc aatccgcaat atcggcagtt ggcattgcgc
      601 tggcgaccct gcgcccggct ggcaaattgc ggggtgcaaa tgagggatgt ggactcgcgc
      661 aacagacgaa tatcggcgcg gccctattgc gccttgatgc ctgcctcgcc gatgaccttc
      721 ccccatttcg ctatttccgt ccggaagcgg gaggcgcagt cctcgggcgt gctggcgacc
      781 ggctcgtaac cgagcgtggc caggcgttcc tgcatgtccg gccgcgagat ggcttgtgca
      841 atctcccgct gcagcagcgc gatgatgtcc ttcggcgttc cggcgggcac gacaacagca
      901 aaccacgatt cgcccgcaac gtcgggataa ccggcttctg tcatggtggg gacgtcgggc
      961 agcgcctgcg agcgggtctt ggtcgagacc gcgaacgccc gcagcttccc gtctttgacc
     1021 agcggcacag ccggcgccgt cgatccgaag gaaatcggcg tgtggcccgc gactgccgag
     1081 gcgatcgccg ggccgccgcc gttgaatggg acgtggatga ggtcgaggcc gagcgacagg
     1141 cggaacaact cgcccacgag atggggcggc gtacctacgc cgggcgaggc gtagctgtat
     1201 ttgccgggat gggctttgac gagggcaacg aggtcgcgga ccgtgtccac cggcagcgcc
     1261 gggttgaccg tgagcaccgc aggtgcggtt accgccagcg tgaccgggtc gaaatccttg
     1321 gtgggatcgt agggcactcg ctggtagagc gcgggattga cgacgtggtt ggcgccgttg
     1381 acgaggagcg tgtagccgtc agggctcgac tgcgccgcgc ggcccgctcc gatattgccg
     1441 cccgcaccgg cgatgttctc gacgtagaat tgaacgccca tttgctcgga cagcttttgc
     1501 gccatgaggc gggcgaacac atcggtcgga ccgcccggcg cgaacggaac gatcacgcgc
     1561 accggtcgtg tgggataggc ttgcgcccta ccgatccgcg gaacggccgc gagcgcggcc
     1621 gcaccggcgg ccaagcgcag aaattggcgg cggggagatg tcatgtgatg cctgttcgaa
     1681 ggttttacca atatccgccg agccagcaaa gtcagggaat ggagaagccg ggctgagcgc
     1741 tgctgtccgc cgaagcagca cccaagcgcg atgggacatc gaccactggg acgctcatgc
     1801 tgctctctgg cttcacgctc tcaaggccca acccattccg cgcgcagccc caataccgcg
     1861 gcgcggcatg gcggtggtgg actttggcaa gtcgacgtga tacgtcgaca tggtcaatcg
     1921 aacgctcgcc agcccggcga cgcgaccact tgcacggacc tctgttctcc ccatcgcttt
     1981 ggtgaagcag cgcctgagcg cgtagccgaa atggttgatg tcagtcagtt tctcgggctg
     2041 cgcgcggcaa agcggacggg cccatcgtcg ccagcggcag gccagctcgg cattttcgcc
     2101 agcgaatctg ggccgattcc tcaacgcgcc gttcgtttca gcgcctggca gatcgccgcg
     2161 ctattcatga cgctggcagc catcgcctcg atcccgatcc tgctttatcc ctggccaccg
     2221 ctgtccgact acatcaatca cctcgcgcgc atgcacgtga tcgccaccat cggcagcgac
     2281 cccaatctgg cgctgttcta ccaggtcgag tggcaggtca ttccaaacct gatgatggat
     2341 ttgatcgttc cgatcctgca gcgggtcatg aacgtctatc tcgccggaca ggtctacacc
     2401 atcatg
//
LOCUS       DATLJT010001137         3443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1460745,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001137 DATLJT010000000
VERSION     DATLJT010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460745"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VM910_26235"
     CDS             complement(<1..363)
                     /locus_tag="VM910_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI66056.1"
                     /translation="MQGAKMQIDLASVVASDFPRLSKPQQSETELIRAIAQGDKRAMH
                     VLFAQHRLRVYRFALRLIDEKEAAEDLVSEAFLNVWRHAGRFEGRCRVSTWLLAITRN
                     LAFSMLRRHRIEKLDDGKA"
     gene            complement(615..3002)
                     /locus_tag="VM910_26240"
     CDS             complement(615..3002)
                     /locus_tag="VM910_26240"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI66057.1"
                     /translation="MLQFSRLLRQRVAGALLVALGALALAAPVAAGRWSLAILGIPLI
                     VLSVTEAYSAFNSPRRADASAYLPCLLALLAGNLLLLSSALVVSGLLILLLAILLIHG
                     FGKILTVWRKPRAVRIPTLANGFVDFGCAALLWYLNRIIGTERAIGIVVGAYIAAAGW
                     RMLLAPVDDATPAAVARTRSAHPDRELGLPPNETFARLRAEAEAASQTVRAADAMWML
                     TLAAVFLAIHLGRMPVSDSLLGITSPFVATGGDVLMTLVLAALLVLPARLLWRRLTRP
                     IERLAWSIRLGAKPGAAPMNRAADWVIGRWLESRLHFAMRLHEGRTSLAAALILVLRL
                     GLPVTAFFVAFNPIWGFTWYFNTESWATGVYQKMTELRVDPWRVAMIDAVTRAYGGDG
                     GNLFRVDPGGLQGGGDFSFLVIGDPGEGDASQYSLVSRYLELGRRDDVKFLVVSSDVI
                     YPAGSMHDYEANFYLPFQGVAKPIYAIPGNHDWYDALEGFNANFLEPKAARAAIEARV
                     EADLRLTSTNAHRIDKLMAEAARLRRQYGVDVGTQRAPFFELQTEDFALLAVDTGVLR
                     TIDQRQWAWIERALDRSRGKFIMAIVGHPLFAGGRGLPPIAEDHDVSESSDNFAALYS
                     LLAKHKVKIAMAGDTHDFEYYREKIRGNGGQVMHHFVNGGGGAYLSIGTALDFPKQPA
                     VGDWAFYPRTDRLHAKMDAETPMWKQPFWYWIKWFNAWPFHVEALSGVFDFNRAPFFQ
                     SFLEVRVERSRRSVVLVLNGVHGPLQWRDLQTSGTVVPADATLDDPVEFIVPMDR"
     gene            3218..>3443
                     /locus_tag="VM910_26245"
     CDS             3218..>3443
                     /locus_tag="VM910_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66058.1"
                     /translation="MIIWFDIHRLSPAVGFVVLLLTLFACSVAVAAEPKRVMLLHSFG
                     RDFKPWSEYAKSIRAELDRQSPWRLDITDHS"
BASE COUNT          721 a         1056 c         1043 g          623 t
ORIGIN      
        1 ggccttgccg tcgtccaatt tctctattcg atgccggcgc aacatcgaga aggccaaatt
       61 gcgcgtgatc gccagcagcc aagttgagac tcgacagcgc ccctcgaatc ttccggcatg
      121 acgccagaca ttaaggaacg cttcgctcac caggtcttcg gcagcttcct tttcatcgat
      181 caggcgcagc gcaaagcggt agacgcggag ccggtgctga gcaaacaaga cgtgcattgc
      241 gcgcttgtcg ccttgtgcaa tcgcccgtat cagctcggtc tctgattgtt gcggctttga
      301 aagtcggggg aaatccgaag cgacgacgct cgccagatca atttgcattt tcgccccctg
      361 cattagctgt gcgaaattgc aggcgcgagc taaccatgat gaggtgattg ctgagtattg
      421 cacttaagga cagtgcgcat atcgatttgg ataaccgaat ataccttggt ttagtgcaag
      481 atgattgccg ctggatcgag tatgcagttg gattcgtgct ccatcaaagc cactcgtgac
      541 ggtctacgtc accattgcga cgatgttcta gttcctgccg gagctgaacc agtaatccaa
      601 atgccgctcg ttcttcaccg gtccattggc acgatgaatt caaccggatc gtcgagcgtt
      661 gcgtccgccg gcacgaccgt gccgctcgtt tgcaagtcgc gccactgcag cggaccatga
      721 acgccgttga ggacgaggac cacgcttcgt ctggatcgtt cgaccctcac ctccaggaag
      781 ctctggaaga agggcgcacg gttgaaatcg aacacgcctg acaaggcctc gacgtggaac
      841 ggccaggcgt tgaaccactt gatccagtac cagaacggct gcttccacat cggcgtctcc
      901 gcgtccatct tagcgtgcag gcgatcggtg cgcggataga acgcccagtc gccgacggcg
      961 ggctgtttcg gaaaatccag cgccgtcccg atgctgagat aagccccacc cccgccgttg
     1021 acgaagtggt gcatcacctg cccaccgttg ccgcggattt tctcccggta gtactcgaaa
     1081 tcatgggtat cgccggccat ggcgatcttg accttgtgct tcgccaacag gctgtagagc
     1141 gcggcgaaat tatccgagga ttcggaaaca tcatggtctt cggcaatcgg gggaaggcca
     1201 cggccgccgg cgaacagcgg atgtcctacg atcgccatga tgaacttgcc gcggctgcgg
     1261 tcgagcgcgc gctcgatcca tgcccattgt cgttggtcaa tcgtgcgcag gacgccggta
     1321 tcgaccgcga gcaacgcgaa gtcctcggtc tgcaattcga agaagggcgc ccgctgggtg
     1381 ccgacgtcga cgccatactg ccggcgcaga cgtgccgcct ccgccatcag cttgtcgatg
     1441 cgatgagcat tggtgctggt cagtcgaaga tcggcttcaa cgcgagcctc tattgccgcg
     1501 cgcgccgctt tgggctcgag gaagttcgcg ttgaaccctt ccagcgcatc gtaccagtcg
     1561 tggttgccgg ggatcgcgta gatcggcttg gcgaccccct ggaacggaag ataaaaattg
     1621 gcttcgtagt cgtgcatcga tccggcggga tagatgacgt cggaggacac caccagaaac
     1681 tttacgtcgt cacggcgtcc cagctccaga tagcgcgaga ccaacgaata ttgcgacgca
     1741 tcgccttctc cagggtcgcc gatgacgaga aagctgaaat caccgcctcc ttgcaggccg
     1801 ccgggatcga ctcgaaagag gttaccgccg tcgcctccat aggcgcgcgt gaccgcatcg
     1861 atcatggcga cacgccaggg atcgacccgc agctcggtca ttttctgata gacgccggtc
     1921 gcccagcttt cggtgttgaa ataccaagtg aacccccaga tcgggttgaa ggccacgaaa
     1981 aacgcggtca ccggcaggcc caatcgcagg acgaggatca gcgcggccgc aagcgatgtg
     2041 cgcccctcgt gcaggcgcat ggcgaaatgt aagcgacttt cgagccagcg gccgatcacc
     2101 caatcggccg cgcgattcat cggcgcggcc ccgggctttg cgccgagccg gatcgaccaa
     2161 gccaaacgct cgatcggcct cgtcagccgt cgccagagaa gacgcgcggg aagcacgaga
     2221 agcgccgcca ggaccagggt catcagaacg tcaccgccgg tcgcaacgaa gggagacgtg
     2281 atgccgagca ggctgtccga taccggcatc cggccgagat ggatggccag gaagacagcc
     2341 gcaagggtca gcatccacat ggcgtcggcg gcgcgaactg tctgcgacgc ggcctccgcc
     2401 tcggcgcgca ggcgagcgaa ggtttcgttc ggaggaagcc cgagctcgcg gtcgggatga
     2461 gcgctccgcg tccgggcgac ggcggcgggc gttgcatcgt cgactggcgc cagcagcatc
     2521 cgccacccgg ctgccgcgat gtaggcgccg accacgatgc cgatggcgcg ttccgtgccg
     2581 atgatccggt tgaggtacca aagaagcgcc gcgcagccga aatcaacaaa tccattggcg
     2641 agcgtgggaa tgcggaccgc acggggtttg cgccagaccg tcaggatctt tccgaagccg
     2701 tggatcagca gaattgcgag aagcaggatc aacaggccgc tgacgaccag ggcggagctc
     2761 agcagcagca ggttgcccgc gagcagcgca agcaggcacg gcaggtaggc gctcgcgtcg
     2821 gcacgtcggg gcgagttgaa agcggagtat gcctccgtca cgctcagcac gatcagagga
     2881 atgccgagga tcgcaagcga ccatcggccc gccgccacgg gcgcggccag ggcaagcgcg
     2941 cccagcgcca ccaacagggc gcccgcgaca cgctgtctca ggagtctgga aaactgcaac
     3001 attgccgtac ccgcttgcag tcaacgcgca acacagcgcc gctgtgaagc gcgaccgtcg
     3061 agctgcgcat cacgagcaat catgacgacc tcgacgggcg gcaaaaacag cctcggctac
     3121 gcaaatgaga ccccacaaat cgcgtcatcc aaatttcccg actgatgtgt aataatatga
     3181 gaactaagtc gacggcaaca ccaacgcgcc tcatcgcatg atcatttggt ttgacatcca
     3241 ccgccttagc ccggctgtgg gatttgttgt tctcctactg acattgtttg cttgttcggt
     3301 cgctgttgcg gccgagccca agcgggtgat gctgcttcat tctttcggac gagatttcaa
     3361 gccctggagc gagtatgcca aatccatccg tgcggaactg gatcggcaat caccttggcg
     3421 attggacatc accgaccatt cgc
//
LOCUS       DATLJT010001138        11756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3298672,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001138 DATLJT010000000
VERSION     DATLJT010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3298672"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VM910_26250"
     CDS             complement(<1..289)
                     /locus_tag="VM910_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI66059.1"
                     /translation="MNNPQANSPQPVPKLPEHLKKKKRAICLAGGGPAAGLHIGVLKE
                     LKEKELEFNKREDVWALSCIGAWVGVIYNQAPKGKEVEETTRFFEEVFRNDP"
     gene            complement(486..5447)
                     /locus_tag="VM910_26255"
     CDS             complement(486..5447)
                     /locus_tag="VM910_26255"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVI66060.1"
                     /translation="MTGGNSSAGVAIVGIACRFPGAADHSAFWRNLCEGVESISELSD
                     QDLLSAGVPGELLRNPSYVKAASLLPDIDQFDAAFFEYSPEEARLMDPQQRLLLEVAW
                     ETFEDAGEAAGRSAKPIGVFTGVGGVVSSYLVDRLPFSADLPGTTGNLTHLGNDKDFS
                     STRVSYKLNLTGPSINVQTACSTSLVAVHLACQAILAGECDMALAGASTVRVPQRVGY
                     MRVKGGILSPDGHCRAFDADAQGTIFGSGVGAVLLKELTAAIADGNHIYAVIRGSAIN
                     NDGADKVSYTASSAAGQTRAMIEAVLMADVSPDDIAYVECHGTGTVVGDPLEIDALTR
                     AFRTRTDRRGFCAIGSVKTNIGHLEQTAGLAALIKTALALKHRTIPPSLNFRNPNPKI
                     DFAASPFFVNTHCRDWPAGDRPRLAAVNSLGLGGTNAFVVLEEPPAASGRTAESDLAL
                     FTLSARNETALRASIERHLTSLSRDQTPLPDICFTSTSGRTHFPVRFAAVVGSKEQLR
                     EALVANNAGGVGSQPTGERQLAFLFSGQASQYARMGAELYRLQPVFQEEVDRCTEIVG
                     SRLGQPLRDVLLSDDADSILIDETAYTQPALFAVQAGLIALWRSWGIVPNVVLGHSVG
                     EFAAAYCAGVYTLQQVLELLVERARLMQSLLRDGVMASVFSDEATVVAAIQQSDRADL
                     ALAALNGPQNTVISGARDSVTTLIAHFEGLGIRCRLLTVSHAFHSQLMRPAVDAFSRM
                     AAAVQGQTPKIAWISTVSAAPMRKPPGAGYWCDHALNAVRFVEGIQALDQTGASDFLE
                     IGPGNTLLALGRQCVKENGKTWLASLSKREELKAILTSLGELYRRGYDVDWDAFNRPY
                     PCRRVPLPTYPFERRRFWIEADASARSAGPLSNGLTGIRLRSALPDAQFESAYSLQRF
                     SYLDDHRIYGMPVLPTTVGVTALRDAACRYFGSDAVEIANLQYREAMVLPESGERIVQ
                     SILTPLGDSVAEFRFASIGTNGADTWRTHMVGVARKDDPARNGEIAVPRFDQIEQRCA
                     NSIPVDRYYETLRALGLEYGASFRAIETLQRGNGEVLTRVRLPAHLSHDGQAGLHPVL
                     LDACLHLYPALVADYGDFTQVAAARRGTYLPISVERFRCANVRAREVWAHGVLRQPAN
                     RDTDVFTIDVVIYQDDGRFAAAIEGLSLKSLPPEALRLTSGTGDSPKQRPEAGSASPS
                     AGPGVAAAMRTQLKEVSGAKRRELLVGFVRQEAMATLGITGTIDATRPLRELGLDSLM
                     SVTLVNRLEATLGIKISTVKLIQGPSIERLVDDILPDVTGTDDDAVTQALQPELSVGV
                     WPNTAGLSADEDAVPQPIVFQPEHSAGSWPTAADPSADGNGRPPRPIAIQPRSHAGRW
                     LIIVGPRAAPRVRLFCFPFAGGGSVVYRSWARFIDPEVEVVAIEPPGRLGRITETPIA
                     NMKEFVEQLVPEMEELLDRPFVFFGHCLGALTMYETARRLIHATKVGPDHLFVSGARP
                     PDQITEQGPFEQRVMHDLLKLAEFRISLPPYAQPDDVFAELIRHFNIQATDQLLSNPE
                     LRQLILPVVRAEFQMATDYQFVREPPWEIPITCFAAKGDPYVSRHHALGWGRFTNLRL
                     QVHIREGAHFAVVDDAAFIHEVINWELQSNLKQTGQQDNVAPW"
     gene            complement(5444..5800)
                     /locus_tag="VM910_26260"
     CDS             complement(5444..5800)
                     /locus_tag="VM910_26260"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVI66061.1"
                     /translation="MLTAPTLAQIESIVHGKIRAVLAERVGEVARLSGTDRLSATLGL
                     SSLDLAFVVAELEAELGVDPFAKLVSITSVRSVGDLVRAYQQAFFPELERSADEHAVA
                     AAVKRAQTRRTRRKQR"
     gene            complement(5794..6531)
                     /locus_tag="VM910_26265"
     CDS             complement(5794..6531)
                     /locus_tag="VM910_26265"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66062.1"
                     /translation="MPQARKVALVSGGSRGLGAQLVSGFLDRHYRVATLSRTKTTFIA
                     DMLRARKGQHFHWGAVDGRDHEALRAFVKDVGHRLGRIDVLVNNAGIAMEGVLPTMRA
                     RDIVEAIEVNLTAALVLAQACSRFMLRQGSGCIVNISSVHAIRGHSGVAVYSATKAAL
                     DGLTRSLARELGPRQIRVNSVAPGYFDSDMVSDMAEEQKRRIARRTPLGRLARIDDVA
                     NAVHFLTSDQASFITGQTLVVDGGITC"
     gene            complement(6556..7938)
                     /locus_tag="VM910_26270"
     CDS             complement(6556..7938)
                     /locus_tag="VM910_26270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI66063.1"
                     /translation="MSPHWLTRRLQSWGDRPALIWSNESWSFRQLCDGRDAWLRQLAQ
                     HGVKPGDTLAICGDYSPKLCALLLAALVNRNIIVPLASATAPRWNRLMQLAQVQFAVR
                     FEGDDSSHVTSFERAVSRPLLRGLKERDAPGLVLFSSGSTGESKASVLDFDRLLVKFE
                     LPRPAYRMLVFLLLDHIGGINTLLYGLCHGGTIVTTRERTPDAVCAAIEARRIELLPT
                     TPTFLRILLIADAIRRYDLSSLKIVTYGTEPMPPSTLAVVREALPWVRFKQTYGLSEL
                     GILPTQSRDSGSVWLKLGNAGFEHKIVDGVLWIRSPSAMLGYLNAPSPFDADGWFNTQ
                     DLVEREGEYIRILGRKSELINVGGEKVHPTEIENILLQVDNVKDVTVRGQPNPVTGEV
                     VAAKITLLGPEDPDTLKRRVRQFCHARLERYKIPAVIDVVLEDHYGARFKKSRDQTSI
                     RRPADTDAGS"
     gene            complement(7979..8389)
                     /locus_tag="VM910_26275"
     CDS             complement(7979..8389)
                     /locus_tag="VM910_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66064.1"
                     /translation="MFFDDAQQHPSESDAPESGIRFAMMLAELWHDWFEAMSEVAYQT
                     HRACEFFVRHGGSPNGQHGRFSIHEPFDFRASRSPYEGPNGSIDMDKLKECLQSLDSM
                     QAARIMHAVQTMQAMEAMLKRRRSRANQAEEAAW"
     gene            complement(8523..9266)
                     /locus_tag="VM910_26280"
     CDS             complement(8523..9266)
                     /locus_tag="VM910_26280"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HVI66065.1"
                     /translation="MNDPRVARPALSLGEVHVWTARLADEHHATADLLRILSREERTQ
                     AAQFSFERDRMRFIQAHGIVLQILSNYLDADAATLIFARTHHGKPYLIPRANGPHLEF
                     SVSRSSNCCMLAVQLDHSIGIDVEKVRDLPQAIDIVRSYFTPAESKALSDLRGAARRD
                     AFFALWTHKEATVKGLGISLAAYLGRIEFDLDPIGGLRLVGWDGDQSVAQKWSVVRLD
                     PAPGYVAAVASAHPIRCLTLHNWNHTGAE"
     gene            complement(9269..10042)
                     /locus_tag="VM910_26285"
     CDS             complement(9269..10042)
                     /locus_tag="VM910_26285"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HVI66066.1"
                     /translation="MTLDKWVPFRNEGPVVRCRLFCFPHAGGNATFYRPLRNFMPPDV
                     DFCPLELPGRAARLDEPPFTSMSALMEPLDRALQPLMGVPFGFFGHSVGACMAFEAAR
                     QLRSVDGRIAVHLFVSGRGSPKFAPADPPQARPPSDQDLLAILGRFGGTPAAVMQRPE
                     LIAALLPALRADLALVAGYAIDPGDHIACPITGFAGADDVAHPGSLQAWRDFTRGKFR
                     TCIFPGGHFYFSPAAGALAREIIEDLYASVGMHAPDARP"
     gene            10202..11188
                     /locus_tag="VM910_26290"
     CDS             10202..11188
                     /locus_tag="VM910_26290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HVI66067.1"
                     /translation="MPTFKAIVVEKSDGGQKIRLTDFDEANLMEGDVTVRVEWSTVNY
                     KDGLAITGKAPVVRRFPMIAGIDFAGTVESSTHPQWKPGDRVILNGWGLGETHLGGYA
                     EKARVKGNWLVRLPARNSARGAMAIGTAGFTAMLAVMALERHGVTPRHGPVIVTGAAG
                     GLGSIAVALLAKLGFAVIASTGRPTEADYLRSLGASEIMERKELVGAAKPLGQERWAA
                     GIDTVGSTTLANVLSMTRYGGAVAACGLAGGMDLPATVAPFILRGVSLIGVDSVMCPL
                     ERRREAWRRLASDLDAVKLAAMTSEIDLSGVMEAGRRIVEGGIRGRIVVKIG"
     gene            complement(11485..>11756)
                     /locus_tag="VM910_26295"
     CDS             complement(11485..>11756)
                     /locus_tag="VM910_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66068.1"
                     /translation="LLIKSLETARRQQALSWQLRTACDLVHLRQGQGRGDQALTLLQS
                     IYDQFTEGFSTADLIRARALLEGLRANSVAKVAKDQSRADAATAD"
BASE COUNT         2385 a         3594 c         3524 g         2253 t
ORIGIN      
        1 acggatcatt ccgaaacacc tcctcaaaaa atctagtagt ttcttcaacc tccttgcctt
       61 ttggggcttg gttatagatg acacctaccc acgcaccgat gcacgacagt gcccatacat
      121 cctcccgctt gttgaattct agttcttttt ctttgagctc ctttagaacg ccaatgtgca
      181 atcctgctgc cggcccgccg cccgcgaggc aaatcgctcg ctttttcttt ttcaggtgct
      241 ctggcaattt tggtaccggc tgtgggctgt tcgcctgcgg attgttcatt tgaagccccc
      301 attgagctcg tgagcaccca cgcccaacaa tgtccacaga aagagctagc ttggatggtt
      361 tagaaactta ccagcgggtt tcccagggtt gcatcgtatc gcgatggccg atccggtcaa
      421 tgaccatttg gtgcctcgag gcgagcatcg cgcgaaaagg ccgaagcgtt gggggaaagc
      481 ggctatcacc acggcgccac attatcctgt tggccggttt gtttcagatt agactgcagt
      541 tcccaattga tgacctcatg gatgaaagcg gcatcgtcca cgactgcgaa atgggcaccc
      601 tcgcggatgt gcacctgcag acgcaagttg gtgaaacgtc cccagcccag cgcgtggtgg
      661 cgcgacacat agggatcgcc ctttgccgcg aagcacgtaa ttggaatttc ccatggcggt
      721 tcgcgcacga attggtagtc ggtcgccatc tgaaattcgg cgcgcacgac cggcaatatc
      781 agctgccgca attcggggtt actgagcagc tggtccgtcg cttgaatatt gaaatggcga
      841 atgagctcgg cgaatacatc gtccggctgt gcgtaagggg gaagactgat tcggaattcc
      901 gccagcttca acaaatcgtg catcacacgt tgttcgaatg gaccttgctc agtgatctgg
      961 tcgggcggcc gcgcacccga gacaaacaag tggtcgggcc cgaccttggt cgcatgaatg
     1021 agccggcgcg cggtctcata catggtcaag gcgcccaggc agtggccaaa aaacacgaac
     1081 ggccggtcca gcagttcctc catttcggga acaagctgtt cgacaaactc cttcatgttg
     1141 gcaattggtg tttcggtgat tcggccgaga cggccgggcg gctcgattgc gaccacctcg
     1201 acctctggat caatgaaccg cgcccagctt cgataaacca cggagcctcc gccggcgaac
     1261 ggaaagcaaa aaagccgaac tctgggagct gctcttgggc ccacaataat cagccaacgg
     1321 ccggcgtgac ttctgggctg aatcgcgatc ggtcgcggcg gccggccgtt accgtcggcg
     1381 ctcggatccg cggcggtcgg ccaactgcca gcagaatgtt caggctgaaa cacaattggt
     1441 tgcggcaccg catcttcgtc ggcgctcagg cccgcggtat tgggccaaac accgacggag
     1501 agctcaggct gaagcgcttg cgtcactgca tcatcgtcgg ttcccgtcac gtccggcagg
     1561 atgtcatcca cgagtcgctc gatgctgggg ccctgaatca gtttcacggt tgaaatcttg
     1621 atgccgagcg tggcctccag ccgattcaca agcgtcaccg acatcagcga atcgagcccc
     1681 agctcacgca gcgggcgagt cgcatcgatc gttccggtga tgcccaacgt cgccatcgcc
     1741 tcctgccgga caaatcccac gagcaactcg cggcgcttcg ctccggacac ctctttcaat
     1801 tgggtcctca tcgccgctgc cacaccgggc cctgcgcttg ggcttgccga accggcttct
     1861 ggcctttgtt tcggtgaatc gccggtccct gatgtgagtc tcaatgcttc cggcggcaat
     1921 gatttcaacg acaatccttc gatggcggcc gcgaaccgac cgtcgtcctg gtagatgacg
     1981 acatcgatgg tgaacacgtc cgtgtcgcgg tttgcaggtt ggcgcagcac gccatgcgcc
     2041 catacctctc gagcacggac gttcgcgcaa cggaaacgct cgacgctgat cggtaagtag
     2101 gtgcctcgcc gcgctgcggc cacttgtgtg aaatcgccat agtccgccac cagtgccgga
     2161 taaagatgca ggcaggcgtc gagaagcacc gggtgcaggc cggcctggcc atcatgggag
     2221 agatgcgcgg gcagccgaac tcgcgtcagg acctcgccgt taccccgctg cagcgtctcg
     2281 atggcgcgga atgatgcgcc atattccaat cccaaagcgc gcaaggtttc gtaatagcgg
     2341 tcgaccggga tagaattggc acaccgttgt tcgatctgat cgaaccgagg aacggcgatc
     2401 tcaccattcc gcgccgggtc gtcctttcgc gccacgccca ccatatgagt tcgccacgta
     2461 tcggcgccat tcgtaccgat gctggcgaag cgaaactcag ctaccgaatc gcccagcggt
     2521 gtgaggatcg attgcacgat ccgctcgccg ctttccggca acaccattgc ctcgcggtat
     2581 tgcagattgg cgatctcgac tgcgtcggag ccgaaatacc ggcacgccgc atcccgcaaa
     2641 gccgttacac caaccgtcgt cggcagcacc ggcatgccat agatgcgatg atcgtcaaga
     2701 taagagaagc gttgcaagct gtaggcgctt tcgaattggg catccggcaa tgccgagcgc
     2761 aagcgtatgc cggtcagccc attggaaaga ggtcccgccg atcgcgcact cgcgtcggcc
     2821 tcgatccaga aacggcggcg ctcgaaagga taggtcggta gcggcacgcg acggcaaggg
     2881 tatggtcgat tgaatgcgtc ccaatcgacg tcgtaaccgc gacggtaaag ctcgccaagg
     2941 ctcgtcagga tcgctttcaa ttcttcgcgc ttgctgagcg aggcgagcca tgtttttccg
     3001 ttttctttca cgcattgtcg tccaagcgcg agcaaggtgt tgcctggccc gatttcgagg
     3061 aagtcggaag cgccagtctg gtccagcgct tggattccct cgacaaaacg cacggcattg
     3121 agcgcgtggt cgcaccaata tcccgcgccc ggcggcttcc gcatgggcgc agcggataca
     3181 gtcgagatcc acgcgatctt cggcgtctgg ccttgcaccg cagcggccat acgactgaac
     3241 gcatctacgg ctggccgcat caactgagag tggaaggcat gggaaacagt cagcaatcgg
     3301 catcggatgc cgagaccctc aaaatgcgca atgagagtcg taacgctgtc gcgcgcgccc
     3361 gagataactg tgttctgcgg cccatttagc gccgcgagcg caagatccgc gcggtcagat
     3421 tgctggatcg ccgccaccac ggtcgcttcg tccgagaaaa cgctcgccat gacgccgtcc
     3481 cgcagcaaac tttgcataag acgcgctcgt tcgacgagca actcaaggac ttgctgtagc
     3541 gtgtacacgc cggcgcagta ggctgccgca aactcgccga cgctgtggcc taggacgacg
     3601 ttgggaacga tgccccagga ccgccataac gcaatgaggc cggcctgcac cgcgaacagc
     3661 gccggctgcg tgtaggcggt ttcgtcaatc agaattgaat ccgcatcgtc gctgaggagt
     3721 acgtctcgga gcggctgtcc cagacggctg cccacgatct cggtgcagcg atcgacttcc
     3781 tcctggaata ccggctgaag gcgatagagc tctgcaccca tccgcgcata ttgcgacgcc
     3841 tgtccggaga acagaaaggc caactggcgc tctccagtcg gttgagaccc cacgccgccg
     3901 gcgttattcg cgacgagtgc ttcacgcaat tgttctttcg agcccacaac ggcggcgaaa
     3961 cgcacgggaa aatgcgttcg gccgctggtg ctggtaaagc aaatatcggg caacggcgtt
     4021 tggtcgcggc taagcgatgt gaggtggcgc tcgatcgatg cccgcaatgc ggtctcattc
     4081 cttgccgaca acgtgaataa ggccaggtcc gactcagccg ttctcccgct cgctgccggc
     4141 ggttcttcga gcacgacgaa cgcgttcgta ccgccgaggc cgagcgaatt gaccgcagcg
     4201 agccgcggac gatcgccggc gggccagtcg cggcaatgtg tgttgacgaa gaacgggctc
     4261 gcggcgaagt cgatcttcgg gtttgggttg cggaagttga ggctgggcgg gattgttcgg
     4321 tgtttcaatg ccaacgcggt cttgatcaga gcggcgagcc cagccgtctg ttccaggtgc
     4381 ccaatgttgg ttttcaccga gccgatcgcg cagaaaccgc gccggtccgt tctggtgcgg
     4441 aaggcgcgcg tcaatgcgtc gatctccagc ggatcaccaa caaccgtgcc cgttccgtgg
     4501 cactcgacat aagcaatgtc gtccggcgac acatcggcca tgagcacagc ctcgatcatc
     4561 gcgcgcgtct gacctgccgc gctcgacgcc gtgtaactca ccttatcggc cccatcgttg
     4621 ttgatggccg accctctgat caccgcgtag atgtgattgc cgtcggcgat ggcagcggtg
     4681 agctccttga gcaggaccgc gccgacaccg ctgccgaaaa tcgtgccttg cgcatcggca
     4741 tcgaaagcac ggcaatgccc gtcgggtgac aggatgcctc cttttactct catatagccg
     4801 acgcgttggg gtacgcgcac tgttgaggct cccgccaatg ccatgtcaca ttcgcctgca
     4861 agaatcgcct ggcaggcgag atgcacggcg accagcgacg tcgaacatgc ggtctgcacg
     4921 ttgatacttg gtccggtcag gttgagtttg taagaaacgc gagtgctcga aaaatccttg
     4981 tcgttgccaa ggtgcgtgag gttgcccgtg gtgcctggca ggtccgcgct gaagggcagg
     5041 cgatcgacaa ggtagctgct gactactccg cccactcccg tgaaaacgcc gatcggcttg
     5101 gccgagcgtc cggccgcttc gccggcgtcc tcgaaggttt cccacgccac ctccagaagc
     5161 agccgctgct gcggatccat gagccgcgcc tcctcgggcg aatattcgaa gaaggcagca
     5221 tcgaactggt cgatgtccgg cagcaatgag gcagccttaa cataggacgg attgcgcaac
     5281 agctcgccag gcactcccgc ggacaggaga tcctggtcgc tcagctcgct gatcgattcc
     5341 acaccttcac aaaggtttcg ccaaaaggcg gaatgatcgg ccgcgccggg aaaacggcaa
     5401 gcgataccga caatcgcgac accggcggag ctattgccgc ccgtcatctc tgctttctcc
     5461 gcgttcgtct ggtctgagct cgtttaaccg ctgccgcgac cgcatgctcg tctgcgctgc
     5521 gctcgagctc gggaaaaaac gcctgctgat aggcccgcac gaggtccccg accgatcgaa
     5581 cgcttgtgat cgaaacgagc ttggcaaatg gatcgacgcc aagctcggct tccaattcgg
     5641 ccacgacgaa cgccaagtcg agcgagctga ggccgagcgt tgcgctgagc ctgtcggtac
     5701 ctgacaggcg agcgacctcc ccgacgcgct ctgccaagac cgcgcggatt ttgccgtgca
     5761 cgatggattc gatttgcgcg agcgtcggcg cagtcagcat gtgatgcctc cgtccaccac
     5821 gagggtctgt ccggtgatga aagacgcttg gtcggaggtc aggaagtgaa cggcgttcgc
     5881 gacatcatct atgcgtgcca gccgacccag cggcgtgcgc cgagcgatgc gccgcttctg
     5941 ctcctccgcc atatcgctga ccatgtcgct gtcgaaatag ccgggagcca cggaattgac
     6001 cctgatctga cgtgggccga gctcgcgcgc aaggctacgc gtcaaaccgt cgagcgctgc
     6061 tttagtcgcg ctatagacgg ccactccgga atgcccgcgg attgcatgca ccgaggatat
     6121 gttgacgatg cacccggagc cctgccgcag catgaaccgc gaacacgcct gcgcgagcac
     6181 gagagctgcg gtgaggttca cttcgatggc ctcgacaata tcgcgcgcgc gcatcgttgg
     6241 tagaacgcct tccattgcaa ttccggcatt gttgacgagc acgtcgatgc ggcccaaccg
     6301 atggcccacg tccttcacga aggcgcgcaa tgcctcgtga tcgcggccgt ccactgcgcc
     6361 ccaatggaaa tgctggccct tgcgggcacg caacatatcg gcgatgaaag ttgtcttcgt
     6421 tcggcttagc gtcgccaccc gatagtgccg gtcgagaaac ccggacacta gttgggctcc
     6481 caagccgcga ctccccccgc taaccagcgc gacctttctg gcctgtggca tggtcgtcgt
     6541 gtccttgcgc tcaacttacg atccggcgtc ggtatcggcg gggcgccgaa tcgaggtttg
     6601 atcgcgcgat ttcttgaaac gcgcaccgta atgatcctcc aggaccacgt cgatgacggc
     6661 gggtattttg tagcgctcga gtcgcgcgtg gcagaattga cgcacgcgcc gtttgagcgt
     6721 gtcgggatcc tccggcccga gcagggtgat tttggctgca acgacctcgc cggtgaccgg
     6781 attcggctgg ccgcgcaccg tgacgtcttt gacgttgtcg acttgcagca gaatgttctc
     6841 gatttcggtc ggatgcactt tctcgccgcc cacgtttatc agctccgact tgcggccgag
     6901 aatgcgaatg tactcgccct ctctttcgac aaggtcctgc gtgttgaacc agccgtccgc
     6961 atcgaacggt gacggagcgt tcaaatagcc gagcatcgcg ctcggcgacc tgatccaaag
     7021 cacgccgtcg acgatcttgt gctcaaatcc agcattgccg agtttgagcc acaccgagcc
     7081 ggagtcgcgt gattgggtcg gcaggatgcc gagttccgaa agcccgtagg tctgcttgaa
     7141 ccgcacccac ggcaaagctt cgcgcacgac cgcaagcgtg ctggggggca tcggctccgt
     7201 tccgtaggtg acgatcttaa gggaggaaag atcgtaacgt ctgatagcgt cggcgatcag
     7261 caagatccgc aggaacgttg gcgtcgtggg cagcaactcg atccggcgcg cttcgatcgc
     7321 ggcgcataca gcgtctggag tgcgctcacg tgtcgtgacg atggtcccgc cgtgacagag
     7381 cccgtacaac agcgtgttga tgccgccgat atggtcgagc agcaaaaaga ccagcatgcg
     7441 ataggccggt cgcggtagct cgaacttcac caatagccga tcaaaatcga ggaccgaagc
     7501 cttgctctcg ccggtcgagc ccgagctgaa tagaaccaga ccgggtgcat cgcgttcctt
     7561 gagtccccgc aacagcggac ggctcaccgc acgctcgaag ctggtaacgt gcgatgaatc
     7621 gtcgccctcg aatcgaacgg cgaattgcac ctgcgccagc tgcatcagcc gattccagcg
     7681 cggcgcagtc gcggacgcca acgggacgat gatgttgcga ttcaccaagg cggccagaag
     7741 caaagcgcac agcttcggcg aatagtcacc acagattgct agcgtgtctc ccggtttgac
     7801 cccgtgttgc gccaactggc gaagccaagc atcacggccg tcacagagct ggcgaaacga
     7861 ccaactctca ttgctccaaa tcagcgccgg tctgtccccc caagattgca atcgccgagt
     7921 cagccaatga gggctcatga ccgcccgccc ctgccgctct gcgttgccat tcaagcctct
     7981 accaagcagc ctcctccgcc tggtttgcgc gcgatcttcg ccttttcagc atagcctcca
     8041 tggcttgcat cgtctgaacg gcatgcatga tccgcgccgc ctgcatggaa tccaacgatt
     8101 gtaggcactc tttgagtttg tccatgtcga tggagccgtt cggcccttca tatggactgc
     8161 gagaggcgcg aaaatcgaaa ggttcatgaa tactgaaacg tccgtgttgc ccgttcggtg
     8221 accctccatg tcggacaaaa aattcacagg ctctgtgcgt ctggtaggcc acctccgaca
     8281 ttgcctcgaa ccaatcatgc caaagctcgg caagcatcat ggcaaagcgg atgcctgatt
     8341 cgggagcgtc agactccgac ggatgctgct gcgcgtcatc gaaaaacatg aggcaccccc
     8401 gtttacacaa cagccccagc gacgttcccg ccccgatggt cttgcttcac cgattgcgtt
     8461 gcaaacattg cacaattcct aatatatgtc cacggctcgc agccgcataa gtagcaccag
     8521 catcactcag cgccggtgtg gttccaattg tgcaatgtca ggcaacggat cggatgcgcg
     8581 ctggctacgg cggcgacgta ccccggtgca ggatcaagac gaacaacgga ccacttttgg
     8641 gcaacggatt gatcgccatc ccacccgacc agccgcaggc cgccgatcgg atcgagatca
     8701 aactcgatgc ggcccagata agccgccaag ctgatgccca atcctttgac cgttgcctcc
     8761 ttgtgcgtcc ataaagcaaa gaatgcatcc cgccgcgccg ctccccgtaa atcactcaag
     8821 gctttgcttt ccgccggtgt gaaatagctt cgcacgatgt cgatggcctg cggcaggtcg
     8881 cgcaccttct cgacatcgat gccgatcgag tgatcgagtt gcaccgcgag catgcagcaa
     8941 ttgctgctgc gcgagacgct gaactcgaga tggggaccgt ttgcccgcgg aatcaaatac
     9001 ggcttcccat gatgagtgcg tgcaaagatc agtgtcgccg cgtccgcatc caaatagttg
     9061 gaaagtatct gtagcacgat cccgtgcgcc tgtatgaaac gcatccgatc acgctcgaat
     9121 gagaactgcg cggcctgtgt gcgctcttct cggctcagga tgcgcagcaa gtcagccgtt
     9181 gcgtgatgct cgtcggcgag acgagcagtc cacacgtgga cttcaccgag cgataatgcc
     9241 ggacgagcta cgcggggatc gttcatatct acggcctcgc atctggcgcg tgcatgccca
     9301 cggatgcata caagtcctcg atgatctccc tggcgagagc gcctgccgca ggtgagaagt
     9361 aaaaatgccc tccagggaag atgcaggtgc gaaactttcc gcgggtgaag tctctccagg
     9421 cttggagaga accgggatga gcaacatcgt cggcgccggc aaatccggtg atcggacaag
     9481 caatgtgatc ccctggatcg attgcatacc ccgccacgag cgcaagatct gctcttaagg
     9541 ccggcagtag agcagctatg agttccggcc gttgcatgac ggccgcgggc gtgccgccaa
     9601 aacggcctag aattgcaagc aagtcctggt ccgacggcgg acgtgcctgg ggagggtcag
     9661 cgggagcgaa tttggggctc ccgcggccgg aaacgaacag atgcacggcg atgcggccat
     9721 cgaccgagcg cagttgccgg gcggcttcaa aggccataca ggcgcccacg ctgtggccaa
     9781 aaaaaccgaa tggcactccc atcagcggct gcagtgcacg atccagcggc tccatcagcg
     9841 cgctcatgga cgtgaacggc ggctcgtcaa gacgcgccgc acggcccggc aactccaagg
     9901 gacagaaatc cacgtccggc ggcatgaaat tccgtaacgg ccggtaaaac gtggcattgc
     9961 cgccggcgtg cggaaagcag aaaaggcggc aacgtacgac cgggccttca ttgcgaaatg
    10021 gtacccactt atcgagcgtc attgcgttca tcgttctgag cggaacagca tgatcaataa
    10081 cggtgcttca ccaaaagatc acgcgcaaga ctcactctca aaggtgctga tcccagacat
    10141 cgagttgcgg cggccggtgc cgcacggtac agcttttcgc acgcatgaat ggaggggata
    10201 cgtgccgaca ttcaaggcta ttgtggtcga aaagagtgat ggcgggcaaa agatccgcct
    10261 caccgatttc gacgaagcca atctgatgga aggcgacgtc accgtgcgcg tcgaatggtc
    10321 gacggtgaat tacaaggacg ggctcgcgat caccggcaaa gcgcccgtcg ttcgccgttt
    10381 tccgatgatt gccggcatcg atttcgccgg cacggtcgaa tcctccacgc atccgcaatg
    10441 gaagccgggc gacagggtca tcctcaacgg ctgggggttg ggcgagaccc atctcggcgg
    10501 ctacgcggaa aaggcgcggg tcaagggcaa ttggctggtg cgattgcccg cgcgcaatag
    10561 cgcgcgcggc gcgatggcga tcggtaccgc cgggttcacc gccatgctgg cggtgatggc
    10621 gctcgagcgt catggcgtta cgccgcgaca cgggccggtc atcgtcaccg gagcggcggg
    10681 aggactgggc tcgatcgcag tggcgctgct cgccaagctt ggctttgccg tcatcgcctc
    10741 gacaggacgt ccgaccgagg cggattacct tagaagcctc ggtgccagcg agatcatgga
    10801 acgcaaggag ctcgtcggcg cagccaagcc gcttggccaa gagcgatggg ccgcaggcat
    10861 cgataccgtt ggatcgacca cactggccaa cgtgctgtcg atgacccgct atggcggcgc
    10921 ggtcgccgcc tgcggattgg ccggtgggat ggatcttccg gcgacggtcg cgccatttat
    10981 cctgcgcggg gtgtcgctca tcggcgtgga ttccgtgatg tgtccgctgg aacggcggcg
    11041 ggaggcatgg aggcgactgg cgtccgatct ggacgcagtc aaactcgcgg cgatgacgag
    11101 cgagatcgat ctgtcggggg tgatggaagc cggtcggcgt atcgtcgaag gagggatccg
    11161 cggtcgaatc gtggtcaaaa tcgggtagag ccggtgaacg cgtgggcgag cgggccgccc
    11221 ggcgcaaatg ttacccatac cgccccgacg gccgacccca actgtgtcct cgccgctgtc
    11281 aaagaagaga agaaaaatta gaatcaatac agcaacgtgc taagaggcag tttcgaaatt
    11341 agttgtcagg cgtgcagctc atcgagcaac cttcaagtcg ccactcagcg gattggtatc
    11401 agcttcccag cccaatcaat gtgcatagac tcgcccccca ctgggccatt gggaatcccc
    11461 gattcccctt gggcggaaaa cgctttagtc ggctgtcgcg gcgtcggcgc ggctttgatc
    11521 cttcgccact ttggccactg agttcgcacg caagccttca agaagcgccc tggcgcggat
    11581 caggtcggcc gtactgaagc cttccgtgaa ttgatcataa atggattgca acaatgtcaa
    11641 ggcttgatcg ccgcgacctt ggccctgccg gaggtgcacg agatcgcacg ccgtgcgcag
    11701 ctgccaggaa agggcctgct ggcgccgtgc ggtttctagg ctcttgatga gcaggt
//
LOCUS       DATLJT010001139         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2378939,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001139 DATLJT010000000
VERSION     DATLJT010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378939"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1564)
                     /locus_tag="VM910_26300"
     CDS             complement(<1..>1564)
                     /locus_tag="VM910_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI66069.1"
                     /translation="TGFVVDDAIVVLENVSRHLEAGMPRLRAALIGAREVGFTVLSIS
                     LSLIAVFLPILLMGGILGRLFREFTLTLSLAILISLAISLTTTPMMCALFLRPRPPER
                     MGERRRSFFERVLAAYERSLVWALRHSRLVLVIFFAAIALNFFLFYVVPKGFFPQQDT
                     GRLVGTLQADQSVSFQLMTQKLRQMMDIVQRDPAVEHVVGYTGVGSGGGFGQINTGSV
                     FVSLKPISQRPPIDDVIARLRPQLAKVPGGRLFLVAVQDIREGGRQANAQYQYTLQSE
                     DTALLQKWTPLLVQQLEHSSVLADVSSDQQQRGLEADLVVDRTTASRLGISLAQIDNT
                     LYDAFGQRQVSVIYSAINQYHVVMEIDPRYTQHPASLEDIYVTPSGGPAAGTAISNAP
                     LGTVRAANATAPGSAATASAAAATSNNNNAARNAFTNAIGNRGKGTASSGAAVSTAPE
                     KMIPLAAIAHYNAGNAPLSVSHQGLFVASTISFNLHPGASLGAAANEITDAINKIGMP
                     TSIHGNLAGTAQI"
BASE COUNT          292 a          474 c          534 g          264 t
ORIGIN      
        1 aaatctgcgc ggtgccggcg agattgccgt ggatcgaggt cggcatcccg atcttgttga
       61 tggcgtcggt aatctcgttc gcggctgcgc cgagcgaggc gccgggatgc aaattgaacg
      121 agatagtgga ggcgacgaac aagccctgat ggctgaccga gagcggcgcg ttgccggcat
      181 tgtaatgcgc gatcgcggcc agcgggatca tcttctccgg agcggtcgac acggccgcgc
      241 ctgacgacgc ggtgcctttg ccgcgatttc cgatggcgtt ggtgaaggcg ttgcgcgcgg
      301 cattgttgtt gttcgacgtc gcggctgccg cactcgccgt cgcggcggag cccggcgctg
      361 tcgcattggc ggcgcggacg gtccccaacg gcgcgttcga gatcgccgtg ccggcggccg
      421 ggccacctga cggcgtgacg taaatgtctt cgagcgaggc tgggtgctgg gtgtagcgcg
      481 gatcgatctc catcacgacg tgatactggt tgatcgcgct gtagatgacc gagacctggc
      541 gctggccgaa ggcgtcgtag agcgtgttgt cgatttgcgc gaggctgatg ccaagccgtg
      601 acgcggtggt gcggtccacc accaaatcgg cctccagccc gcgctgctgc tgatcggagc
      661 tgacatcggc gagcaccgaa ctgtgttcga gctgttgcac cagaagcggt gtccatttct
      721 gcaacagcgc cgtatcttcg gattgcagcg tgtattgata ctgcgcattg gcctggcggc
      781 cgccctcgcg gatatcctgg accgcgacca ggaacagccg gccgcccggc actttggcga
      841 gttgcgggcg cagccgcgcg atcacgtcgt cgatcggcgg gcgctgcgag atcggcttta
      901 aggaaacgaa taccgagccg gtattgatct ggccgaagcc gccgccgctg ccgacgccgg
      961 tatagccgac cacgtgctcc accgcgggat cgcgctgcac gatgtccatc atctgacgga
     1021 gcttctgcgt catcagctgg aacgacacgc tctgatcggc ttgcagcgtc ccgaccagcc
     1081 gcccggtatc ctgctgcgga aagaaaccct tcggcacgac atagaagaga aaaaaattga
     1141 gcgcgatggc ggcgaagaaa atcaccagca ccagccggct gtgccggagc gcccagacca
     1201 gcgagcgctc gtaggcggct agcacgcgtt cgaaaaaact gcggcggcgc tcgcccatcc
     1261 gttccggtgg ccgcgggcgc aggaacagcg cgcacatcat cggcgtcgtg gtcaacgaga
     1321 tcgccagcga gatgagaatg gcgagcgata gcgttagcgt gaattcgcgg aacagccggc
     1381 ccaaaatgcc gcccattagc aggatcggga ggaacacggc gatcagcgac aggctgatcg
     1441 agagcacggt gaagccgacc tcgcgcgcgc cgatcaaagc ggcgcgcagc cgcggcatgc
     1501 cggcttcgag atggcgcgag acattttcca gaacaacgat ggcatcgtcg accacgaagc
     1561 cggt
//
LOCUS       DATLJT010001140         9453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1230822,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001140 DATLJT010000000
VERSION     DATLJT010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230822"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VM910_26305"
     CDS             complement(<1..1026)
                     /locus_tag="VM910_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66070.1"
                     /translation="MNLPRRRFLHLAAGAAALSAVSRIAMAQTYPSRSVRLIVGAPAG
                     GGFDIVARLMGQWLSERLGQSFVIENRPGAGSNIATEAVVRALPDGYTLLLVTSVNAI
                     GTSLYEKLSFNLSRDIAPVAGITRVHDVMVANLSVPSRTVPEFIAYAKANPGKLTMAS
                     GGVGTPSHLEGELFKTMTGVDMLHVPYRGTAPSLTDLLGGQVQVTFTSMPSVIEYIRA
                     GRLRALAVTTATRSEALPDIPTVGEFLPGYEGGIWYGLGAPKATPAEIVNKLNKEVNA
                     ALDDPKMKARLTDLGGTVLPGSPADFGKHIAEETEKWGKVIRAAGIRARVGAVRSRAN
                     VHNSAASR"
     gene            complement(1036..1305)
                     /locus_tag="VM910_26310"
                     /pseudo
     CDS             complement(1036..1305)
                     /locus_tag="VM910_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            complement(1330..2310)
                     /locus_tag="VM910_26315"
     CDS             complement(1330..2310)
                     /locus_tag="VM910_26315"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66071.1"
                     /translation="MKRRQFITLLGGVAVAWPLAARAQQPAMPVIGFLSGLSPADRTR
                     FVTAFRQGIRETGYVEGQNVAIEYRWALDQYDRLPDLAADLVRRQVAVIAAHDTQSAI
                     AAKAATTTIPIVFTTGGDPVKDGLVACLNRPGGNVTGVSFIAAELGTKHLGLLRELRP
                     GAARIALLVDPRWPLTERFVSEVRAAALTIGQQIEVFYTSSGREIDTALASLSQTLVD
                     AILVGPSPFAYSRRAQLVTLAAYHRVLAIYFQREFVEIGGLMSYGTSIVDANRLAGMY
                     TGRILKGEKPGDLPVMQSIKFEFVINLNTAKAFGLSFPPGLLAIADEVIE"
     gene            complement(2307..3350)
                     /locus_tag="VM910_26320"
     CDS             complement(2307..3350)
                     /locus_tag="VM910_26320"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66072.1"
                     /translation="MKRREFITLLGGAAAWPLTAFQPMLLKLCWIVLTCLILIGTTVV
                     PSQAQRRAPTVGILNYAATGDVRVVQFLTALRELGYIEGRNIALVQRHADGALDKLPG
                     LAAELVAAHVDLIIALGPAVWAAKQATATIPIVIAFSGNPERQGVVASLARPGGNLTG
                     FSYMSADLAGKRLELLCSAVAKCSRIAILYNPEEPATTAELEEAAATARSLGVTIAPA
                     AVRRVDELEQAFGVAARDQVEGMVVLTHGFAVLNRTRIMELAARLRLPVLYGWREFAE
                     EGGLMSYGPDIQVLVRQAATYVDRIIRGEKPSNLPVQQPTKFELVINLKTAKALGLTV
                     PDSLLARADEVIE"
     gene            complement(3362..3562)
                     /locus_tag="VM910_26325"
     CDS             complement(3362..3562)
                     /locus_tag="VM910_26325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66073.1"
                     /translation="MSTAEHIYSIADNHVQTVQVCIDRLHELGANEQLKLLTGRLRKL
                     AVRATPGNKDTKAAGIVVRVAV"
     gene            3852..5060
                     /locus_tag="VM910_26330"
     CDS             3852..5060
                     /locus_tag="VM910_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66074.1"
                     /translation="MRKPFAVFGFAAAIVGSTPAGAQISNDIVKIGVLSDMSSLYSDS
                     TGPGSLIAAQMAAADFNGNVKAKPIEVIGADHQNKPDVGSSIAREWYDTGKVDVIVDV
                     PTSSVALAVQQITRDKNHVFLMSGTASSDLTGPACSPNGIHWTYDTYALGKVAGNAMV
                     ARGEDTWFFITVDYVFGHTLERDTASVVTAAGGKVFGAVRAPINTQDFSSFLLKAQAS
                     QAKVIGLANTGSDMQNAIKQASEFGLQRGGQKLAALLFDVTDAHSLGLQVAGGMIVTA
                     GFYWDMNEETRAFSNRFMQRAKRMPTMFQAGVYSALMHYLKAIDATGTDDAKAVVARM
                     KATPVNDFFAKNGRIREDGRMVHDMYLMQLKSPAESKGSWDVYKLLATVPGDQAFRPL
                     QEGGCPLIKR"
     gene            5177..8515
                     /locus_tag="VM910_26335"
     CDS             5177..8515
                     /locus_tag="VM910_26335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66075.1"
                     /translation="MECLSCQADNPESGRFCTQCGAALPAKCIQCGAVNSPKNRFCGS
                     CGASLAVATRTSNGFTAQTARPPSPSKTNLISGGERRHLTVLFCDIVGSTSLARSLDP
                     EDLNEITRRYYDCCTDAIHQFDGLVANYIGDGVMALFGYPRAHEDDAERAIRAALTII
                     SAVRAVNPKSKARVRVRIGIATGLVVVGEDGTEPLTKEKTVVGEAPNLAAHLQSESQP
                     NNVLISDATRRLVGNVFSLEELKLGRLKGGKEPVTVWRVIGEKEVTSRFAAHAVSFTN
                     FVGRDQEVALLTDRWHQAIQGEGQVLVLLGEAGIGKSRIVETFRELVSNESHITLRYQ
                     CSPYHIDSALHPIIRQLEHAAGLTAEDSASVKLDKLEALLRRSNTPLEEVIPLFAALL
                     SIPTDGRCPPPNPDPQRRKERTLNALIDQLAGLAQAEPVLIILEDVHWADPTTLEFLG
                     RTILRLSEMRVLLIMTGRPEFKVRWAQHPQVTALLLNRLARQHCRAMVESIAQKTLPS
                     EVMEQIIAKTDGVPLFVEELTKTVLESGLLQEKDRAWLLTEPLLELAIPATLQDSLMA
                     RLDRLAPVKEVAQIGASIGREFSYSLLAAVSSMREAELKDALAKLAAAELIFPHGAPP
                     AASYIFKHALVRDAAYETLLRSKRKQLHGRIAKVLETHFPETAETQPEILAHHYTHAG
                     LTEIALQWWQKAGDLAIRRSANREAVSHYDRVIELLRLLPASPKRDASELVTRIKLSG
                     PLIATAGYITSELTENYARAWELCTELGDDKHAFPVMYGQWVIPYVRGNMAEALKNSE
                     RFLRRAEQQDDVSLLMMGHRIYGSSLVWRGDTVQGSSHLKRALSIYRPEHAQLAYVFS
                     QHPRTAALAHLCLALQHLGHLDQAMAAGWEAIEEAKRIEHFNSIAYSLCFVSLLIMLR
                     LDVATLKRTSGELLELAEQHNATYWARWARPMLGWIEAQEGNIEAGIHQMQQSTAELQ
                     EEGANLWVPQTLLLEAEILGRAGQGQNAHRLLDEAQALIEPLDQRFYEAELHRVRGMV
                     MLAEGNDVDAAAANFDRAIEVARCQSSRFLELRAATSKARLCLERGRHQSAHDLLAPV
                     YSSFSEGLDTADLVEARSLLNELM"
BASE COUNT         1918 a         2830 c         2835 g         1870 t
ORIGIN      
        1 ccggctcgcg gcggaattat gaacgttggc ccgcgaacga accgccccta ctctggccct
       61 gatgccggcc gctcggatca ccttgcccca cttctcggtc tcctcggcaa tgtgtttgcc
      121 gaagtcggcg ggcgagcctg gaagcacagt gccaccaagg tcggtaaggc gcgccttcat
      181 cttgggatcg tcgagagccg cgttgacctc cttgttgagc ttgttgacga tttcggccgg
      241 cgtcgccttc ggtgcgccga ggccatacca aatacccccc tcgtagcccg gcaagaactc
      301 acccacggtc gggatgtccg gcagcgcttc cgagcgcgtc gctgtggtta ctgcgagcgc
      361 acgcagcctc ccagccctga tgtattcgat caccgagggc atggaggtga acgtcacctg
      421 cacttgtcca ccgagcaagt ccgtcagtga aggggcggtg ccgcgataag gtacatgcag
      481 catgtcgacg ccggtcatcg tcttgaacag ctcgccctcc agatgggagg gggttccgac
      541 accacccgac gccatggtga gcttgccggg attggccttg gcataggcga tgaactcggg
      601 gaccgtcctc gatggaaccg agaggttcgc taccatgacg tcgtggacac gggtgatgcc
      661 agcaaccggc gcgatgtcgc ggctgagatt aaaactaagc ttttcgtaca acgaagtacc
      721 gatcgcgttt accgaagtga cgaggagaag cgtatagccg tccgggagcg cgcgcacgac
      781 cgcctcggtg gcgatattgc tgcccgcgcc cggccggttc tcaatgacga atgactggcc
      841 gagccgttct gacagccatt ggcccatcag gcgtgcgacg atatcgaatc cgcctcctgc
      901 gggggcaccg acaatcaaac gcaccgaccg cgacgggtaa gtttgcgcca tcgcaatgcg
      961 tgacaccgcc gagagcgcgg cagcgcccgc agccagatgc agaaatcgtc ggcgcggcag
     1021 gttcatggcg tttagccccc cggcaggcgc acgcacgacc gcttcggtgg cgatattgga
     1081 gctcgcccct cgccgattct cgatgacgaa cggctggccg agccgttctg acagccattg
     1141 gccgattagg cgtgcgacga tatcgaatcc gcctcctcgg ggggcaccga caatcaaacg
     1201 cacgggccgc gacggatagt tctgtgcccg tgcgatgcgc gaaggtgccg ccagcgcggc
     1261 agcgcctgcg gctaggtgca gaaaatttcg gcggggaagt ttcattttga acctccctct
     1321 ccgttcacat cactcgatca cctcgtcggc gatggcgagc agccccggtg gaaagcttag
     1381 gccgaatgcc ttggcggtgt tgaggttgat cacgaactcg aacttgatcg actgcatgac
     1441 cggtaggtcg ccgggctttt cgcccttgag aatgcggccg gtatacatac cagctaggcg
     1501 atttgcatcg acgatgctcg ttccgtagct catcagacca ccgatttcga caaactcacg
     1561 ctggaaatag atcgcaagca ccctatggta agccgctagc gtaacgagct gtgcgcggcg
     1621 gctgtaggca aaagggctgg gaccgaccaa gatcgcatca actagcgttt gcgaaaggct
     1681 cgcaagggcc gtatcgatct cgcgaccact gctggtgtag aagacttcga tttgctgccc
     1741 aatggtcaaa gccgcagctc ggacctctga gacaaagcgt tccgtaaggg gccatctggg
     1801 atcgacgagt aaggcaatgc gcgcagctcc cggccgcagc tcacgcaaga gcccaaggtg
     1861 cttcgtgcca agttccgctg cgatgaaact gacaccggtg acgttgccac cggggcggtt
     1921 gaggcaggcg acgaggccgt ccttgaccgg gtcgcccccg gttgtgaaga cgattggtat
     1981 cgtcgtggtt gcggccttag ccgcaattgc cgactgggtg tcatgcgcag cgatcactgc
     2041 tacctgacgg cgtaccaaat cagctgcgag gtcgggcagt cgatcgtatt ggtccagcgc
     2101 ccagcgatac tcgatcgcca cgttctggcc ctcgacatag ccggtctcgc gaatgccttg
     2161 gcgaaatgcg gtcacaaacc gtgttcggtc cgcgggtgac aagccgctga gaaacccgat
     2221 caccggcatc gccggctgct gcgcgcgcgc cgcgagcggc cacgcaacgg ccacgccgcc
     2281 gaggagcgtg atgaactggc gacgtttcat tcaatcacct cgtcggcgcg ggcgagcagg
     2341 ctatccggca cggtgaggcc gagcgccttc gcggttttga ggttgatgac aagctcgaac
     2401 ttggtgggct gctggacagg gaggttgctc ggtttttcgc cccttatgat gcgatctaca
     2461 tacgtcgcgg cttgcctaac caggacctgt atatcagggc cgtacgacat caggccgccc
     2521 tcctcggcga attcgcgcca cccatacagt actggcagcc gtaggcgtgc agccaactcc
     2581 ataatgcgcg tacggttcag caccgcaaag ccgtgcgtca acaccaccat tccttcgacc
     2641 tggtctcgcg cagcgacgcc gaaagcttgc tccaattcat cgactcgtcg gactgccgca
     2701 ggcgctatgg tgacgccgag actacgggca gtcgccgccg cttcttcgag ctctgctgtc
     2761 gtcgccggtt cctcgggatt gtagagaatg gcgatgcgac tgcatttggc caccgcactg
     2821 caaagtagtt cgagccgctt tccggccaag tctgcggaca tgtatgagaa gccggtgaga
     2881 tttccgccag gccgcgcgag gctcgctacc acgccctgcc tttcaggatt gccactgaat
     2941 gcgatgacga tcggaattgt cgcggtcgct tgttttgccg cccatactgc tggccccagg
     3001 gcgatgatca gatcgacatg ggccgcgacc agctcggcgg ccagccccgg cagcttgtct
     3061 agcgcgccat ctgcgtgtcg ttgaacaagt gcgatatttc ttccctcgat ataaccaagc
     3121 tcgcgcaacg cagtgagaaa ttgaacgaca cggacgtcgc cagttgcggc atagttgaga
     3181 ataccaacag tcggagcgcg tcgctgcgct tgagatggga caacggtggt cccaatgagt
     3241 attaagcagg taagcacgat ccagcacagc ttcaggagca tcggctggaa tgccgtgagc
     3301 ggccacgccg ccgcgccgcc aagcagcgtg atgaactcgc ggcgcttcac tcgatccttg
     3361 gtcagacggc gacgcggacg acgatcccag cggccttagt gtctttgttt cccggtgtgg
     3421 ctcggacggc caactttcgc aaccttccgg tcaacagttt gagttgctcg tttgcgccga
     3481 gttcgtgcag ccggtcaatg cagacctgaa ccgtttgaac gtgattgtcg gcgatactgt
     3541 agatgtgttc ggcggtgctc atgatcgttt gcaatctcgt tcttagccaa ccctaggaat
     3601 gctccgtcat cgggctggtc gcaccccatc tcacagccag ggtgtcgaag ctagcgtggt
     3661 cgccccacgc catgtgtgag ctacttcaca catgtcagca cgaagtactg agtgcttcat
     3721 atggagctgg ggtccgcaag tgtgataaat gttggacagt acgcaatgct ccgcgcccct
     3781 ttttgacaaa atgaaccaac cgtgaggccc gccatttcag ctacgtgcaa cgtcggccat
     3841 tgggagcgca gatgagaaag ccattcgccg tgtttggctt cgctgcggca atagttggtt
     3901 cgacccctgc gggtgcccag atctccaatg atattgtcaa gatcggggtg ctctccgata
     3961 tgagcagcct ctactccgac tccaccggcc cgggctcact cattgccgct caaatggcgg
     4021 ctgcggattt caacggcaac gtgaaagcta agccgatcga ggtaatcggt gccgatcatc
     4081 agaacaagcc ggatgtgggc tcgagcattg cgcgcgagtg gtacgatacc ggcaaggtcg
     4141 atgtcatcgt cgatgttccg acctcgtcag tggcgctcgc cgtccagcag attacccgag
     4201 acaagaatca cgtattcctg atgtccggca cggcctcttc tgatctcacc ggtccggcct
     4261 gctctccgaa tggcattcac tggacgtacg atacttacgc cctcggcaag gtggccggca
     4321 atgccatggt cgcgcgcgga gaagatacct ggttcttcat cacagtcgat tacgtgttcg
     4381 gccatacgct ggaacgcgac acggcgagcg tcgtcaccgc ggcaggcggg aaagtctttg
     4441 gtgcggtgcg tgcgccgatc aacactcagg acttctcctc gtttctactg aaggcgcagg
     4501 catctcaagc caaagtcatc ggactggcca ataccggcag cgatatgcag aacgctatca
     4561 agcaggcatc cgagtttgga ctccagagag gtggacagaa actagcggct ctactgttcg
     4621 acgtcaccga tgcgcatagt cttggcctcc aggtggccgg ggggatgatc gttacagcag
     4681 ggttttattg ggacatgaac gaagagacac gcgcattctc caaccgcttt atgcagcggg
     4741 ccaagcgcat gccaactatg ttccaggcgg gcgtctattc ggcactcatg cattacctta
     4801 aagccatcga tgcgaccggc accgatgatg cgaaggcggt cgtggccaga atgaaggcca
     4861 cgccggtcaa cgattttttc gcaaagaacg gacgtatccg cgaggacggc cgcatggtgc
     4921 acgacatgta tctgatgcag cttaagtcgc cggccgaatc gaaagggtcc tgggatgtgt
     4981 acaaattgct cgccactgtt ccgggcgacc aagcctttcg gccgctgcaa gagggtggct
     5041 gtccgctgat caaaagataa aaacgagcag caaccgaagg gttccgcggc ggggggcggg
     5101 aaatggtcta ctgagctttt cggcccgttg cggatatcgg caaaacatcc acggctataa
     5161 gcaactggac cgcttcatgg aatgcttgag ctgtcaagcg gataatccgg agagtgggcg
     5221 tttctgcacg caatgtggcg ccgcgctgcc cgccaaatgc attcaatgcg gggctgtgaa
     5281 ctcaccaaaa aatagatttt gcggaagctg tggcgccagc ttggcggtcg ctacgcggac
     5341 ctcgaatggt ttcaccgcgc agacagcgag gccgccgtcg ccaagcaaaa ccaatttaat
     5401 ttccggcggc gagcgacggc acctcacggt tcttttttgc gatatcgtgg gttcgacatc
     5461 tctcgccagg tcgctcgatc ccgaagatct caatgagatc acgcgtcgct attacgactg
     5521 ttgcaccgac gcgatccacc aattcgacgg cctagtcgcg aattacattg gcgatggcgt
     5581 catggcactt ttcggttatc cacgcgccca cgaggacgat gccgagcggg cgatccgcgc
     5641 ggcgctgacc atcattagcg ctgtccgagc ggtaaatccc aagagcaagg cgcgcgttag
     5701 ggtccggatc ggtatagcaa ccgggcttgt cgtcgtgggt gaagacggca ccgagccctt
     5761 gaccaaagaa aaaacagtgg tcggtgaggc acccaatctt gcagctcatc tccaatccga
     5821 atcacaaccg aataatgttc tgatttccga cgcaacgcgt cggctcgttg gcaacgtttt
     5881 cagcttggag gaactgaagc tcggccgttt gaagggcggc aaggagccgg tgaccgtttg
     5941 gcgcgtgatt ggagagaagg aggtaacgag ccgtttcgcc gcccacgccg tgagcttcac
     6001 gaacttcgtc ggccgggatc aggaagtggc cctgctgacc gatcgctggc accaggcgat
     6061 tcagggtgag ggacaagtcc tagtgctttt gggagaggcg ggcatcggca agtcgcgcat
     6121 tgtcgagacg ttccgcgagc tcgtctccaa cgaatcgcat atcacgctgc gctatcagtg
     6181 ctccccttat cacatcgata gtgcgctcca tccaattatt cgccagctcg agcatgccgc
     6241 aggtctcacg gcagaggatt ccgcatcggt gaagctcgat aagctggagg ccttgctgcg
     6301 gcgcagcaat accccgttgg aagaggtcat tccgcttttt gcagcactgc tctcgatccc
     6361 cacggacggc cgctgtccgc ccccgaaccc cgatccgcag cgccgcaagg aacgcacgct
     6421 gaatgcgctc atcgatcaac ttgcaggtct ggctcaggcg gagcccgttc tcatcatcct
     6481 cgaagacgtg cactgggccg acccgaccac cctcgagttc ctcggtcgta cgatcctgcg
     6541 gctttcggag atgcgcgtcc tcctgatcat gactggccgg ccggagttta aagtccgctg
     6601 ggcccagcat ccgcaagtga ccgcgcttct tctgaaccgc ttggcgcgcc agcactgtcg
     6661 ggcaatggtc gagtcgatcg ctcaaaagac tctgccgtcc gaggtgatgg agcagatcat
     6721 cgccaagacc gacggcgtgc cgctgttcgt cgaggaactc actaagacgg tgcttgagtc
     6781 gggattgttg caggagaagg atcgcgcctg gctccttaca gagccgctgc tcgagcttgc
     6841 cattccggca acgctgcagg attcgctcat ggcgcggctg gatcgacttg caccggtcaa
     6901 ggaggtggca caaatcggtg ccagcatcgg tcgcgagttc tcctattccc tgctggcagc
     6961 ggtgtcctcc atgcgcgaag cggagctcaa ggacgcactg gccaagctcg ccgctgccga
     7021 gcttattttc ccacacggcg caccgccggc tgcaagttac atcttcaagc acgcactcgt
     7081 gcgcgatgcc gcctatgaga cactcctgag gagcaaacgg aaacagcttc acgggcggat
     7141 cgcaaaggtt ctggaaaccc attttcccga aaccgcggag acacaacccg aaattctcgc
     7201 ccaccattac acgcacgcgg gtctgaccga aatcgcgctg cagtggtggc agaaagcagg
     7261 cgacctcgcg atcaggcgct cggccaatcg cgaggcggta agccattacg atcgcgtcat
     7321 cgaactcctt cggctgttgc cggcaagccc caagcgcgat gcgagcgaac tggtgacgcg
     7381 aatcaaactg agcggcccct tgatcgcgac agctggctac atcacttctg aattgaccga
     7441 gaattatgcc cgagcctggg agctctgcac cgagctcggc gatgacaaac atgcctttcc
     7501 cgtgatgtac ggccagtggg tcatccccta cgtgcgcggc aatatggcgg aggcgcttaa
     7561 gaattccgag cggttcctgc gccgagccga gcagcaagat gacgtcagcc ttcttatgat
     7621 ggggcaccgg atctacggct cgtcgctcgt atggcggggt gacaccgtgc aggggagctc
     7681 acatctcaag cgcgctctat ccatatatcg ccccgagcac gctcagctcg cctacgtttt
     7741 cagccagcac ccgcgcacgg cggcgctggc gcacctctgc ctcgcgctcc agcatctggg
     7801 ccatctcgat caggcgatgg ccgccggctg ggaagcgatc gaggaggcga agcggatcga
     7861 gcatttcaac tcgattgcct attcgctgtg cttcgtgagc ctcctcatca tgttgcgcct
     7921 ggacgtcgcg acgctcaaac gcacctcagg ggaacttctc gagttggccg agcagcacaa
     7981 tgccacctat tgggcgcggt gggcgaggcc gatgctaggc tggatcgagg cccaggaggg
     8041 aaatatcgaa gccggcattc accagatgca acagagcacg gcggagctgc aggaggaggg
     8101 ggccaatctg tgggtgccgc agaccctgct gctcgaggcc gaaattctgg gtcgcgcggg
     8161 gcaaggccag aatgctcacc ggctgctgga tgaggcgcag gcgctcatcg agccgctcga
     8221 ccagcgcttc tatgaggcag agctccaccg cgtccgcggc atggtcatgc tcgccgaagg
     8281 taacgacgtc gatgccgcgg ccgccaattt cgatcgtgca atcgaagttg cgcgctgtca
     8341 gagctcgcgt ttcctcgagc tgcgcgcggc cacaagtaag gcgcgactct gcctcgagcg
     8401 cggtcgccac cagtctgcac atgatctgct ggcgccggtc tacagctcct tctccgaggg
     8461 cctggacacg gctgatctcg tcgaagcacg atcgctgctg aacgagctga tgtgatatcc
     8521 tttcacagca gaatcttatg tactaatttt gagaattcgt agctgctgtt ggctcaacaa
     8581 ctatcggaaa ttggttcggg tttcatgcca tcgccctaac aggtccctcc gtgctaggct
     8641 gagggtgcgg gagacccacc gcacggggtg ggacggcggc gcggctgctt tgtcgccgac
     8701 ctaagataag tccgagcggc aggctgatct atcttggcca tcattggcga gcttgcatcc
     8761 gcaggtatgg catgtgcgga tgcgagcgcc tggtctagct gccacacctt catgtctttg
     8821 ccgcaggctt ggcctaacca gagttccatt gcttattttg tctcgactcg ctctgggtaa
     8881 aatcgcagaa ttagagcgac cgcgacgcta ccccaaagcg ttcggcacca catccgactt
     8941 gttgtcttgg gaccccttcg agcgtcccgt gtcctcgctc cgacgcatgt cgcgcgaaag
     9001 aattgccttc aatggttggc tgttcagcag ccgagcaatc agggaggagc ctatgccccg
     9061 gtataagtgc aaaagcatcg ctgcgctcga cattgctctc ggcggcttgc cagccaagat
     9121 gcaggttgag gcggacgtag gcaccggcgt gtctgcaaag accgttggag agcttcggag
     9181 ggtgacgact tggtccgaga acttggtgct aacaatcccg caggaacgtc atcccgaaag
     9241 cgcagtaaag atcagcaagg ctagcgtagc gacacgcacc tcgccgaaac cgtagtcctc
     9301 tcgcgacgga taacctctcg cgagagttca acataacaat atgtgtcaat gggaggaatg
     9361 ctatggccag caaaccagac gcgtcgatgc catttctaaa cttggggaac gagcaaacag
     9421 aggcgatgct aagtatgcaa aatgaactcc ttg
//
LOCUS       DATLJT010001141         7376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2149340,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001141 DATLJT010000000
VERSION     DATLJT010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149340"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1322)
                     /locus_tag="VM910_26340"
     CDS             complement(6..1322)
                     /locus_tag="VM910_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66076.1"
                     /translation="MILSVFLPFAAGYYLAYLFRTINAAISPALVSDFGLDAAETGLL
                     ASIYFLVFGLTQIPIGVLLDRFGPRRVQGVLLVIAAGGATLFGTASSFPELLVARAMI
                     GLGVAGALMAGLKAIVTWFPGERVVLANGWMIMLGSFGAVTATAPTDWLLNYFGWRSL
                     FEVLTIGTFAVSGLIYMVVPKRVADSEIATPARNPLTLWSIYSDPRFLRVAPLSAACI
                     GSSWALQSLWAAAWLTDVEGFDLHSRAAQMFFMALGLSLAALLLGAMADRLRKHNIRK
                     EVLLGGVGALFVVAELALVLRVPVPSLLPWTIVSIAGAATVLSFAVIADYFPREFAAR
                     ANGALNLLHFGWAFMVQYGIGLVVGQWPSQNGHYPVVAYQAAFGLSLALQTAALVWFA
                     MPWIWTFGKNFCVAFAEPHIVRGSAAGSGGVPIDGPILEACEGVEW"
     gene            complement(1432..2193)
                     /locus_tag="VM910_26345"
     CDS             complement(1432..2193)
                     /locus_tag="VM910_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66077.1"
                     /translation="MKTSRCGSSLVSFRSDGLISCRTWRNRSVCPWRRGGMVMQLRSE
                     LEMQAGQAEAKAPPIPERKPKRTRKVTVRLKEELYNRLSVATERPGFGKSMVVEAALE
                     RFLSPAPSVEDVVQERFDDMDVKFDRLERDVRMMAETIALHTRYHLSVIPPLPQSRQQ
                     EAALLGDERFKVLAEQVDRRVRLGQPLMQETIDRLNFGHGAAKDASQDPGLEYNDQKP
                     APEEVDIKPASFAAAGEGGSNSYFRRRPSAFSRSA"
     gene            complement(2252..2689)
                     /locus_tag="VM910_26350"
     CDS             complement(2252..2689)
                     /locus_tag="VM910_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVI66078.1"
                     /translation="MRTKHTFRLPPDLARQLADYAGRKRVHQAFVVETALASFLSPDS
                     SEKMEAALGKRLDRLTRQVERLERHVTISNEAMALFVRFWLTATSPVPDAALPAAQAK
                     GRERYERFVEALGRRLAKGQSLAQEISLDAEPQSKQSSESGLA"
     gene            complement(2694..4700)
                     /locus_tag="VM910_26355"
     CDS             complement(2694..4700)
                     /locus_tag="VM910_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HVI66079.1"
                     /translation="MSSTKVLWGQTCVVLGIVLLTMWAATQWVAWKLGFQPRLGPPWF
                     EPISGMPIYPPPAFFWWWYAYDAYAPRIFLQGACIAASGGIISMIVAFAMSIWRAREA
                     KTAATYGSARWARVSEIRASQLLGQDGVVLGRFGGNYLRHNGPEHVLCFAPTRSGKGV
                     GLVIPTLLTWPGSCVVHDIKGENWNLTSGFRARHGRVLLFDPTNRRSAAYNPLLEVRR
                     GEWEVRDVQNIADVLVDPEGALERRNHWEKTSHSLLVGAILHVLYAEEDKTLAGVANF
                     LSDPRRPIEATLNAMMTTAHPGSSGPHPVIASAARELLNKSENERSGVLSTAMSFLGL
                     YRDPVIALVTRRSDWRIRDLVEAKRTASLYLVVPPSDISRTKPLIRLILNQIGRRLTE
                     DLDAQKRRHRLLLMLDEFPALGRLDFFESALAFMAGYGLKSFLIAQSLNQIEKAYGPN
                     NSILDNCHVRICFATNDERTARRISDALGMATELRAMKNYAGHRLSPWLGHLMVSRQE
                     TARPLLTPGEVMQLPPEDELVLVSGCPPIRAKKARYFEDERFRERVMPPPKPGGTAEL
                     HDKGNDDWSGLDLPTTNIAEPSDVNRPRNAEPVNDQANSGIRREPELPEHEEVVPESG
                     PVKAEFDFADEEADDEVQRASTLRKQGTDIARQAAMDPGDGLGL"
     gene            complement(4936..6498)
                     /locus_tag="VM910_26360"
     CDS             complement(4936..6498)
                     /locus_tag="VM910_26360"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HVI66080.1"
                     /translation="MADLRTFTRDLMRQVEKDLETHLDWIAIDHYNTGHPHTHIIVRG
                     VLDGGGILNIAGDFIAHGIRYRASEMVTLELGHQSEIELQSKLKTEVEAERWTRLDKM
                     LATEQRERGIVDLMPGGGTTYAFRENRGLMVGRVKFLERYGLSSEVETGQWVISDRAE
                     ATLKELSDRNDIIKTMHRALATHGLEEDRGVDQYVRHGDRPNEKIAGRVLAKGLAGDE
                     MDERVCLVIDGIDGRVHHVEFPDAAHLTDIGRDMLVEVGPTISGPRAADRNIAFNTGQ
                     KDGLYRPSQHLGRIREQFEREGRDPESFVRSHVRRLEALRRVGHVERLDDDHWRIPGD
                     IIERGQAYDLAKGGDGPRIRTLSPQNLEQQIGSDAATWLDRELTAREPLVIADSGFGR
                     DVKDALHRRAERLVKMGDATVREGAIQIPVQTVANLKEREVERAGRRMATERGLTFSP
                     SKAGEYVSGRLTGVASLASGRFAMIEDGLGFRLVPWQPVLEKRIGQFITGVQRESAGI
                     EWDFGRKRGLGL"
     gene            complement(7071..>7376)
                     /locus_tag="VM910_26365"
     CDS             complement(7071..>7376)
                     /locus_tag="VM910_26365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66081.1"
                     /translation="EEGKIKRTGVRELADRSAVEKRLSAHWFLEAPWLAVSDIPSRPD
                     GAFRAFRGISMLGATALADFNLQISAFHLISCASLRFCALDVLQLLLWAYPTRIVGT"
BASE COUNT         1512 a         2270 c         2107 g         1487 t
ORIGIN      
        1 tctgcttacc attcgactcc ttcgcacgcc tcaaggatag gcccgtcgat gggcacgccg
       61 ccggatccgg cggcgctacc gcggactatg tgaggttcag caaatgcaac gcagaagttt
      121 ttgccgaacg tccagatcca tggcatcgca aaccacacca gcgcagccgt ttgaagtgcg
      181 aggctgagac cgaatgcggc ttgataggcc acgaccggat aatggccatt ttgagatggc
      241 cattgtccga ccacgagccc aatgccgtat tgaaccatga atgcccagcc gaaatgcaag
      301 aggttcagag cgccattggc gcgcgcggca aattccctgg ggaagtaatc ggctatcacc
      361 gcaaagctta acactgttgc cgcaccagcg atcgacacga tggtccaagg cagaagtgac
      421 ggcacgggaa cgcgcaagac cagcgcgagt tcggccacca cgaacagcgc ccccacgccc
      481 cccaataaga cttcctttct tatattgtgt ttgcgtaggc gatcggccat ggcaccaagc
      541 agcagggctg caaggcttag gccaagagcc ataaagaaca tttgggccgc ccggctgtgg
      601 aggtcgaatc cctccacgtc cgtaagccag gctgcagccc acaatgactg caacgcccaa
      661 gacgaaccaa tgcaggccgc cgacagcggc gcgactctca ggaagcgcgg atctgaatag
      721 atggaccaca acgtaagtgg atttcgagcg ggcgtggcga tttctgaatc agctacgcgc
      781 ttcggaacga ccatataaat gagtccactt accgcgaacg tcccgatcgt caggacttcg
      841 aacaggctcc gccaaccaaa ataattcagc aaccaatcgg ttggcgccgt cgccgtgacc
      901 gcgccgaacg atcccaacat gatcatccag ccgttggcga gcacgacgcg ctctccagga
      961 aaccaggtga cgatggcctt gagtccggcc atcaacgcgc ccgcaacgcc aagcccaatc
     1021 atggcgcgcg caacaagaag ctctgggaaa ctcgacgcgg tgccgaacag cgtggcgccg
     1081 ccagccgcga tgacgagcag cacgccttgg actcgacgcg gcccaaagcg atccagaagg
     1141 acccctatgg gaatttgggt caacccaaag acgaggaaat agattgacgc gagcagcccg
     1201 gtctcggcgg cgtcgagccc aaaatcggag actaacgccg gcgagatcgc ggcattgatc
     1261 gtccgaaaca ggtaggcaag gtagtaaccc gcagcaaagg gcagaaaaac tgagaggatc
     1321 aagcgccatt ttgaaggcgg ggacttctgg gagacctccg cctcgcttgt taactccgga
     1381 tcggattcgg aaggccgcgg ctgtggagtc ggttttccac ttccggcggc atcacgccga
     1441 ccggctgaat gcgctcggac ggcggcgaaa gtacgaattg ctgccgccct ccccggcggc
     1501 agcgaacgat gcgggtttga tatcaacctc ttcaggagca ggtttctggt cgttgtattc
     1561 taggccgggg tcttgtgatg cgtccttggc tgcgccatgt ccaaaattga gacggtcgat
     1621 cgtctcctgc atcaagggct gacccagccg aacccgcctg tcgacctgtt cggccagaac
     1681 cttgaagcgc tcgtcgccaa gaagtgccgc ctcttgttgc cgcgattgcg ggagaggtgg
     1741 gatgaccgag aggtgatatc gagtgtgtag cgcgatggtt tcggccatca tccgcacgtc
     1801 tcgctcgagc cggtcgaact tgacgtccat gtcgtcaaat cgctcctgca cgacatcttc
     1861 aactgacgga gccgggctca gaaagcgttc aagcgctgct tccacgacca tgctcttgcc
     1921 gaaccccgga cgttcggttg caacggagag tcgattatag agttcctcct taagcctgac
     1981 ggttactttg cgggttcgct taggtttacg ttcgggaatc ggcggagctt tggcttcggc
     2041 ttgcccggcc tgcatctcga gttcgctgcg gagctgcata accattcctc cccttcgcca
     2101 aggacacacc gatcgattgc gccatgttcg gcacgagatc aggccatcac ttctgaacga
     2161 aacaagagaa gaaccgcatc gacttgtttt caagctgacg gaggtgtcgg tcgtattctg
     2221 gcgtagtaaa agaaggaaat ggcgccggcg ttcaagcgag cccagattcg cttgattgct
     2281 tgctttgggg ctcggcgtcc aacgatatct cctgtgcgag actctggccc ttagctagcc
     2341 ttcgtcccaa ggcctcgacg aacctttcgt agcgttcccg tcccttggcc tgcgcagcag
     2401 gaagcgcggc gtcaggtaca ggcgacgttg cggtcagcca gaaccgcacg aatagtgcca
     2461 tcgcttcgtt ggagatcgtg acatgacgtt cgagccgctc gacctggcgc gtcagccggt
     2521 ccagccgttt cccaagcgcc gcttccattt tctcggaact gtcgggtgac agaaacgagg
     2581 ccagcgccgt ctcgacgaca aatgcctgat gcacgcgctt cctgccggca tagtcggcga
     2641 gttgccgggc aaggtcgggc ggcagacgaa atgtgtgctt ggtccgcatt atctcatagg
     2701 ccgaggccgt cgccgggatc catcgcagcc tggcgtgcga tatcggtacc ctgctttctt
     2761 agagtgcttg cgcgctggac ctcatcatcc gcttcttcgt cggcgaaatc gaattcggcc
     2821 tttactggac cagattctgg tacgacttct tcatgttcgg gcaattccgg ctcacgccga
     2881 atgccactgt tggcttgatc gttgaccggc tcggcattgc gcggtcggtt cacgtcacta
     2941 ggttcagcga tgttggtggt gggcaggtca agtccactcc aatcatcgtt ccctttatcg
     3001 tggagctctg cggtgccgcc aggttttggc ggcggcataa cgcgctcgcg aaatcgctcg
     3061 tcttcgaaat agcgcgcttt cttggcgcga atcggtgggc atcccgaaac caggaccaac
     3121 tcgtcctcgg gcggcaactg catcacctcg cccggcgtca gcaagggacg cgcggtttct
     3181 tgccttgata ccatcagatg ccccagccat ggactgagcc tgtgaccagc gtagtttttc
     3241 atggcgcgca gttcggttgc cattcccagc gcatccgaaa tacgccgcgc cgtccgctcg
     3301 tcgttggtcg cgaagcaaat gcggacgtgg caattgtcga ggatcgaatt gtttggaccg
     3361 taggccttct cgatctgatt gagcgactgg gcgatcagga agctcttcaa cccataaccc
     3421 gccataaacg ccaatgcgga ctcgaagaag tcgagcctgc ccagagcggg aaattcgtcc
     3481 agcatcagca gcagccgatg ccgacgcttt tgagcatcga gatcttccgt caagcgacgg
     3541 ccgatttgat tgaggatcag gcgtatcaat ggcttggtcc gactgatatc ggacggcggc
     3601 acgaccagat acaaagaggc cgttcgcttg gcttcgacca gatcgcggat tcgccaatcg
     3661 ctacgtcgcg ttaccagcgc tatgacagga tctctataaa gaccgaggaa cgacattgct
     3721 gtcgaaagca cgccagaacg ctcgttctcg ctcttgttca ggagctcgcg tgcggccgag
     3781 gcgatgacgg gatgcggacc ggaactgcca ggatgcgctg tcgtcatcat ggcgtttaat
     3841 gtggcctcga ttggccgcct gggatcggac aggaaatttg cgacccctgc cagggtcttg
     3901 tcctcctccg cgtaaagcac atgcaggata gcgcctacta gcagcgagtg gcttgtcttc
     3961 tcccaatgat tgcgtcgttc cagggctcct tcgggatcga caagcacgtc cgcgatgttc
     4021 tggacgtcgc gcacctccca ctccccacgg cgaacctcca ggaggggatt ataggcagca
     4081 gatcttcggt tggtcggatc gaacagcaag acgcgaccat gccgagcgcg gaagcccgag
     4141 gtcagattcc agttctcgcc ttttatgtca tggacaacgc aactacccgg ccaggtcaga
     4201 agggtcggaa tgacaaggcc aacccctttc ccgcttcggg tcggagcgaa acacaggacg
     4261 tgctccggtc cattatgccg gaggtaattg ccaccaaagc gcccgagcac gacgccatcc
     4321 tggcccaaga gctgcgacgc cctgatctct gacaccctgg cccaacgcgc cgagccatag
     4381 gttgcggccg tcttcgcctc gcgcgctcgc cagatcgaca ttgcaaaagc aacgatcatc
     4441 gaaatgatac cgcccgatgc tgcaatgcag gcgccttgca gaaatattct tggcgcgtag
     4501 gcgtcatacg cgtaccacca ccagaaaaac gcgggtggag gatagatggg catcccacta
     4561 atcggctcaa accacggtgg gcctaaccgc ggctgaaacc ccagcttcca ggcaacccat
     4621 tgtgtcgccg cccacatcgt gaggaggacg atgccaagca cgacgcaggt ttggccccaa
     4681 agaactttgg tcgaagacat cagtgcaagg tgcgaggttt ttgcgaccgt tcaagaagga
     4741 ttcgcccgca gtcgtacacc ttcgggtcaa agcgtagaga tcggatagat cggtcccagg
     4801 tgataagcta cgctctttaa ggcggacgct accgcccata gcaacgatgg acgatgtaaa
     4861 gccgctcatc ttcgagcacg acatcctcct cgttgaagaa ccgtgaatgc gtgctggtat
     4921 agtttctgag cccgatcata atcccagccc acgtttcctt ccaaagtccc attcgatgcc
     4981 ggcgctctcg cgttggacgc cggtaatgaa ttggccgatg cgcttttcca aaacaggctg
     5041 ccaaggcacc agccgaaagc cgagcccatc ctcgatcatg gcaaagcgcc cacttgcaag
     5101 tgaggcaacg ccagtaagcc ggccgctgac atattcgccc gctttgctcg ggctaaaggt
     5161 caggcctcgc tcggtagcca tccgacgacc ggcgcgctcg acctcccgct ccttaagatt
     5221 tgcgacagtc tggaccggga tctggatggc gccttctctg acggtggcat cgcccatttt
     5281 gacgagacgc tcggcgcggc gatggagagc gtccttaaca tcgcggccaa agccactgtc
     5341 agcaatgacc agaggctccc gagccgtcag ctcgcgatcc agccaagtgg cggcgtcact
     5401 gccaatctgt tgttcgagat tttgtggcga aagggtccta attctcgggc catcgccgcc
     5461 tttggcgaga tcgtaggctt ggccacgctc gatgatgtct ccggggatcc tccaatgatc
     5521 gtcatcgagg cgctccacgt gccccactcg acgcaaggcc tccaaccgtc ggacatggga
     5581 ccggacgaat gactcggggt ccctgccctc tcgttcgaat tgttcgcgga tgcgacccag
     5641 gtgctggctc ggccggtaga gtccgtcctt ctggcccgtg ttgaaagcga tgttgcgatc
     5701 ggcggctcgt ggtccggaga tggtcgggcc cacctctaca agcatgtccc gtccgatgtc
     5761 cgtgagatga gcagcgtcgg ggaattcgac gtggtggacg cgaccgtcga ttccatctat
     5821 caccagacag acccgctcgt ccatctcgtc gccggccaat cccttggcca ggacgcgacc
     5881 tgcaatcttc tcgttcggcc ggtcgccgtg gcggacgtat tgatcgaccc cgcgatcttc
     5941 ttccaggccg tgggttgcga gcgcccggtg cattgtcttg atgatatcgt tgcggtcgga
     6001 aagctccttt aaggtggcct ccgcgcggtc agagatgacc cattgcccgg tctcaacctc
     6061 acttgacaag ccatagcgtt ccaagaactt cacccggccg accataagcc cgcggttctc
     6121 gcgaaaagca taggtcgttc ctcctcccgg catcaggtca acgattcccc tctcgcgctg
     6181 ctcggtagcg agcatcttgt cgagccgggt ccaccgctcc gcctcaacct ccgtcttcaa
     6241 cttggactgc agttcgatct cgctctggtg tccgagttcc agcgtcacca tttcggaggc
     6301 ccgatagcgg atgccgtggg caataaagtc tccggcgata ttgaggatac cacccccgtc
     6361 caggacaccg cggacgatga tgtgggtatg gggatggccg gtgttgtaat gatcgatcgc
     6421 gatccagtcg aggtgtgtct ccaggtcctt ctccacctgt cgcatgaggt cgcgcgtaaa
     6481 agttctcaga tcagccattt cacgggaatc ctcgggcgcg acaataaagc ggaattgatg
     6541 ccgatcttca cggccccgct cgacaaaggc cttgctgtcg gcctcgttct cgagagccga
     6601 ataggccttt cctttctcgc cgtcccggtt gaccccatcc cgttcgagat agcgaagatg
     6661 ggcatcgacc gcccggctcg ccgccttggc cttcttgggg ccatgcgatc cccgcttccg
     6721 cccttgggcg ttgagcctga cgaccctcgc cttgaccgcg acgcggcgtg accggaagcg
     6781 gccggcggaa tcgcgttgcc acccgccgtc atgaccctca cgaagaaata agggaacgac
     6841 tttcgcgcca cgacctcgtg cattgaaccg accggttctt cctcccttgc acgcttcacc
     6901 tgggctcggt ccgcgcccaa tcctgttgga atctcctccg gctctcctga cggccgcttg
     6961 aacttgcttc agaaaaggtt gggactgcag acttccgcgc ggattgatcc gcgtcccccg
     7021 gctgcccacg cgtccgggcc gtacatgaaa atcgtcgctt tgatccgctg tcatgtacca
     7081 acgatcctcg tcggataggc ccatagcaag agctgtaaca cgtcgagcgc gcaaaatcgc
     7141 agtgacgcgc acgagatcaa atgaaaggcg gagatttgca aattgaagtc cgcaagcgcc
     7201 gtcgctccga gcattgaaat tcctcgaaag gctcgaaagg cgccatccgg ccttgatgga
     7261 atgtcgctaa ccgccagcca tggcgcttct aaaaaccaat gtgcagacaa acgtttctcg
     7321 acggctgacc ggtcggcgag ttcgcgaacg ccagttcgct ttatcttgcc ctcctc
//
LOCUS       DATLJT010001142         3540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3069126,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001142 DATLJT010000000
VERSION     DATLJT010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3069126"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="ppk2"
                     /locus_tag="VM910_26370"
     CDS             complement(<1..720)
                     /gene="ppk2"
                     /locus_tag="VM910_26370"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HVI66082.1"
                     /translation="MTSNADARFHRINGEMLDAIDEELEMEIDDDRLAKLLTELAEHP
                     QPETLDRRVYFKELLRLQGELVKLQDWVVQQKLKVVVIFEGRDSAGKGGVIKRITQRL
                     NPRICRVAALPAPSQRERTQWYFQRYVTHLPAGGEIVLFDRSWYNRAGVERVMGFCTE
                     EQYQEFFHSVPEFERMLVRSGTFLLKYWFSISDEEQQFRFLMRIHDPLKQWKLSPMDV
                     ESRARWEQYTKAKEIMLERTHI"
     gene            complement(878..1852)
                     /locus_tag="VM910_26375"
     CDS             complement(878..1852)
                     /locus_tag="VM910_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI66083.1"
                     /translation="MSDPGTPTLDQLRVFLTVVEVGSFAGAARRLGRATSVISYSIAN
                     LEAQLGVLLFDRESTRKPQLTEAGRAVLSEARTVTNGINGLRAKVRGLLHGLEAEIHL
                     VLDVMLPASRVVDALKAFRAEFPTVSLRLYVEALGAVTQMVLNRTATIGICGPLDVDV
                     AGLERIGVGSVELIPVAAPDHPLARAGRNAPGAARDHIQLVLTDRSPLTQGQDLGVIG
                     LQTWRLADLGSKHMLLKEGIGWGYMPEPMVREDLERGRLVQLDMREYKDGFIRLHAIY
                     RTDAPPGPAGSWLIARFEAQAVQAAAAASRRAISSAPPDQRKRSPRAR"
     gene            2010..2498
                     /locus_tag="VM910_26380"
     CDS             2010..2498
                     /locus_tag="VM910_26380"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66084.1"
                     /translation="MLNVPASMHRAPAIKHQPRSKRYYFDVGIGCVCLSGADTGGAYC
                     LLDIGLAPGMAVPRHMHTREDEAYYVLSGELEVVVGDEVFILRAGDTLIAPRDIPHQL
                     RNSGDVENHYLIMFSPSGFEGFLKATAVPAPDNAVAPTEPPAVAVRNVHQLAADYGIL
                     FG"
     gene            2698..3489
                     /locus_tag="VM910_26385"
     CDS             2698..3489
                     /locus_tag="VM910_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI66085.1"
                     /translation="MSESPVLITKDARGVATVAINRPQVRNAIDDHVIHSLTDAFKTL
                     GADGETRIVVLTGRGVAFSAGADLDWMRRMAAGREAENLASAKTISAMLRALNELPKP
                     TIARVNGAAYAAGIGLVAACDIVVAAEDAVFSISEVRIGLVPSTISPYVLSAIGARAA
                     RRYFLTGEPFSAADACRLGLVHKVVAQTGLDEAIEGVISALLAGGPRSQSRAKRLIAE
                     VAGRPVDDALEALTARSIAEARASPEGREGIEAFLNKRKPAWRHE"
BASE COUNT          652 a         1115 c         1104 g          669 t
ORIGIN      
        1 gatgtgagtg cgctccaaca tgatctcttt ggctttggtg tattgctccc agcgcgccct
       61 ggactcgacg tccatggggc tgagcttcca ttgcttgagt ggatcgtgga tgcgcatgag
      121 gaagcgaaat tgctgctcct cgtcgctgat cgaaaaccag tacttcagca ggaaggtgcc
      181 tgaacgcacc agcatgcgct cgaattcggg gaccgaatgg aagaactctt ggtattgttc
      241 ttccgtgcag aatcccatca cccgctcgac gccggcgcgg ttgtaccagc tgcggtcgaa
      301 caggacgatt tccccgccgg cgggaagatg cgtcacgtag cgctgaaaat accactgggt
      361 gcgttcgcgc tggctgggag ccggcagcgc cgcgacccgg cagatccgcg gattgaggcg
      421 ctgcgtgatc cgcttgatga ccccgccctt tcccgccgag tcgcggccct cgaagatgac
      481 cacgaccttg agcttctgct gcacgaccca gtcttgcagt ttcaccagct cgccttgcag
      541 gcgaagcaac tccttgaagt agacgcgacg atcgagcgtc tccggctggg gatgctcggc
      601 caactcggtc agcagcttgg ctaggcgatc gtcgtcgatc tccatctcga gttcttcgtc
      661 gatggcgtcg agcatctcgc cattgatgcg atggaaccgc gcatccgcgt tactggtcat
      721 ggctggaacc caggatttgg cgttggcctc ggcctgttac attcgacgcc tatgacgatt
      781 gtgtgacagt gacggtcgcg cgacgcgaga cccgggacag cgcgctaaag caggcacttg
      841 gcgccgaatg ttgcaaccga ccgcaccgca ctgccgctca tctcgcgcgt ggtgatcttt
      901 tgcgctgatc gggcggcgcg gaagagatgg cgcgtcgact ggcggctgcg gccgcttgta
      961 ccgcctgcgc ctcgaaccgg gcaatgagcc aggagcccgc cgggcccggc ggcgcatccg
     1021 tccggtagat cgcgtgcagc cgaatgaagc cgtccttgta ctcgcgcata tcgagctgga
     1081 cgagtcgccc tcgttcgaga tcctcacgca ccatcggttc gggcatatag ccccaaccga
     1141 tgccctcctt cagcagcatg tgcttcgaac cgagatcggc taggcgccag gtctggagcc
     1201 cgatcactcc aagatcttgg ccctgcgtga gcggtgagcg gtccgtcaat acaagctgga
     1261 tgtggtcacg cgctgcgcct ggtgcattgc gccccgcgcg cgccagcggg tgatcgggag
     1321 ccgcaacggg aatgagctcg acgctgccga ccccgatgcg ctcaagcccg gcgacgtcca
     1381 catcgagcgg tccgcagatt ccgatcgtgg ccgtgcggtt gagcaccatc tgcgtcaccg
     1441 ccccgagcgc ttcgacatag aggcgcagcg acaccgtcgg aaactcggct cgaaacgcct
     1501 tcagcgcatc gaccacgcgc gaggccggca gcatcacgtc aaggacgaga tggatttccg
     1561 cctcgaggcc gtggagaagg cctctcacct tggcgcggag cccgttgatg ccattggtga
     1621 cggtgcgtgc ctccgacagg accgcgcggc cggcttcggt cagctggggc ttgcgcgtcg
     1681 actcgcgatc gaacagcaag accccgagct gcgcctccag atttgcaatc gaatagctga
     1741 tgaccgaggt cgcgcggccg agccttcgcg ccgcgcccgc gaagctgccc acctcgacca
     1801 cggtcagaaa gacccgcaac tggtcgagcg tcggcgtgcc gggatcggac atatcgattt
     1861 tctcgaacgt tatggttcag attatgccag tttcgtagac gggccaacca ccttagatcg
     1921 tgcttcacaa cagtgttgaa cccgagcaca tcgcaccacg gctcgcggca acacgcgctc
     1981 cgggtgcgac aagagaacag gagtcatgca tgctcaacgt tccagcctcc atgcaccggg
     2041 ctcccgcgat caagcatcag ccgagatcca agcgttatta cttcgacgtg ggcatcggtt
     2101 gcgtatgttt atcgggcgcc gataccggag gagcatactg tctcctggac attggcctcg
     2161 cgccaggaat ggcggtacct cgtcatatgc atacgcgtga ggacgaggcg tactatgtcc
     2221 tctcaggaga actggaggtc gtcgtcggcg acgaggtctt tattctcagg gccggcgata
     2281 ctctcatcgc tccgcgcgac attccacacc agctacgcaa ttccggagac gtcgagaatc
     2341 actatctcat catgttctcg ccttccgggt tcgagggctt tctgaaagcg acggcggttc
     2401 ccgcaccgga caatgccgtt gctccgactg agccgccggc agttgccgtt cggaatgtcc
     2461 atcaactcgc cgccgactat ggaattcttt tcggttgatt gatggagctt gagcgccggc
     2521 agcaaacaat gggagtagga tgcacagcag ggcgcaactg taagtcttgc gctctcgatg
     2581 cgaagttttg cacaaaacga gcgtcgacgc gacctcatag cggtttgcgg attgcgagaa
     2641 atagtgcagt ctgacaattc ccgtcgttga aaggctccga tggcggagac gcccgagatg
     2701 agcgaatcgc ccgttctcat caccaaggac gcgcgcggcg tcgccaccgt cgcgatcaac
     2761 cgtccgcagg tgcgtaacgc gatcgacgac catgtcatcc attcgctgac cgatgcattc
     2821 aagacgctcg gcgcggacgg cgaaacgcgg attgtcgtcc tcacgggccg cggcgtagcg
     2881 ttttcggccg gcgccgatct cgattggatg cgacgcatgg cggcgggccg cgaagcggag
     2941 aatttggcga gcgcaaaaac gatcagcgcg atgctgcgcg cgctcaatga gctgccaaag
     3001 ccgaccatcg ccagggtcaa tggcgcggcc tatgccgcgg gcatcgggct ggtcgcggcc
     3061 tgcgatatcg tggtcgctgc cgaagacgcg gtgttctcga tctcggaggt gcggatcggc
     3121 ctcgtgcctt cgacgatcag cccttatgtg ctctcagcca tcggcgctag agcagcccgc
     3181 cgctacttct tgaccggcga gccgttttcc gccgcggacg catgccgatt gggtctcgtg
     3241 cacaaagtcg ttgcacagac cggcctcgac gaagcgatcg agggagtgat ttcggcgcta
     3301 ctcgccggag gtccacgctc ccaatcgcgc gcgaaacggc tgatcgccga ggtcgcgggc
     3361 cgcccggtcg acgacgcgct cgaggctttg accgcgcgca gcatcgccga agcgcgcgcg
     3421 tcgcccgaag ggcgcgaagg catcgaggca tttctcaata agcgcaagcc cgcctggcgg
     3481 catgagtagg ctgcagcccg tcatgcgcgg gcttgacccg cgcatccatc ttcttggcag
//
LOCUS       DATLJT010001143         5161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_81751,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001143 DATLJT010000000
VERSION     DATLJT010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81751"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..479
                     /locus_tag="VM910_26390"
     CDS             306..479
                     /locus_tag="VM910_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVI66086.1"
                     /translation="MDKDRIKGSVRQAKGALKEVVGKVAGDAKLEAEGKSDKVAGKAQ
                     NAVGSLKDALRGK"
     gene            585..1817
                     /locus_tag="VM910_26395"
     CDS             585..1817
                     /locus_tag="VM910_26395"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66087.1"
                     /translation="MADLSLKPSLVPLHAWLIEAGTSDIATETLFDGFCARLTAAGVP
                     IARGFLSIAGLHPLWRAHSLTWNDGRIVEVTDFSHAIMATALWQASPFRHMLETKTRR
                     LHRRLAGDDAVLDFEVLREFRDAGMSEWLALMYGFGWSAARPATNQFGVVLSWTTSTA
                     VGRGNGWSPLELAAIEELSGTLALAVKGNVAPAAAHDLLAAYLGSDAADRVSAGQVQR
                     GSVSRIAAAILCADVRGFTNFAEETAPEEVTRRLNDIFDCLGDPVRAAGGEILKFLGD
                     GVLAVFLPPDGANVAVVTPAVLAAAREILARVEALNATETAAGNPALSLDIALHAGEV
                     TYGNIGTADRLDFTVIGPAVNEAARLEGLCKVLGKPILISNSFAQAAEGVQEPLLSVG
                     RHPLRGVREAHEVFTIDT"
     gene            complement(2011..2361)
                     /locus_tag="VM910_26400"
     CDS             complement(2011..2361)
                     /locus_tag="VM910_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI66088.1"
                     /translation="MVVKRIVPNIASDQTDKAKSFYAEILEMDVVMDLGWIVTFAAAS
                     NANPQVSVAIEGGSGTPVPDLSVEVDNFDEVQLRVRRSGPAIVYGPVQEPWGVKRFFV
                     KDPLGRLLNILAHE"
     gene            complement(2448..2627)
                     /locus_tag="VM910_26405"
     CDS             complement(2448..2627)
                     /locus_tag="VM910_26405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66089.1"
                     /translation="MAITIMGGLFVATAPTLLVVPALYALWFRVRSDETIDAPAFYPR
                     RMESSGGLQRIRIAA"
     gene            2814..3401
                     /locus_tag="VM910_26410"
     CDS             2814..3401
                     /locus_tag="VM910_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI66090.1"
                     /translation="MGKFDRYAFSAQPSFDEAATRKAFSKAVPLKTLVIYCYDPRAAE
                     IPNAVAKLFGDEVFPGDIILDGSGNRVASTTTIFPVIVAGGRAVDALRSITIAQHLFG
                     IQNIVVVHHSHCGGTSFTADGIINAYEHEHHVDISKLYDRSSICISDYETSLKHDTAL
                     VRSHGGTPKNVNVFGYFYDIDTGALTEVVRDVRRT"
     gene            3503..3880
                     /locus_tag="VM910_26415"
     CDS             3503..3880
                     /locus_tag="VM910_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI66091.1"
                     /translation="MAARAVTRLYDDTLRSVGLRATQLAVLAAVGGDEVVSIAGLAKF
                     MGMDRSTLTRNLAPLEREGLIQVGAEGWRRSRTIEITKKGRSPLGEALPLWEKAQETL
                     QRRLGIRRWADIRADLDDLVRGA"
     gene            complement(3886..4272)
                     /locus_tag="VM910_26420"
     CDS             complement(3886..4272)
                     /locus_tag="VM910_26420"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI66092.1"
                     /translation="MNNCRSVRESCAPADLADEALLRSYQADEWPRWFAATGVACPAI
                     KGTMFDSSATMAEAAAQGAGVALLPVAMFERELSLGRLVQPFDTKVSIGSYWLTSLKS
                     KAETDAMRAFRSWIVAAASSSSACGE"
     gene            4856..>5161
                     /locus_tag="VM910_26425"
     CDS             4856..>5161
                     /locus_tag="VM910_26425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66093.1"
                     /translation="MTDRRPHIVVVEDEAAQRHLLVEYLGRHNFRVTGVDGGPALRRL
                     VERELPAVVLLDVGLSGEDGFVLARWLRERSGRLGIIMVTAASDTVDRVVGLETGADD
                     "
BASE COUNT          983 a         1558 c         1659 g          961 t
ORIGIN      
        1 tgcccgtcct gatctggcgg ctgctcgatg aggaacggtt cctgcgacag aatcttgcgg
       61 gctacgcgga atatcaaaca aaggtgaggt atcgcctcct gccatgcatc tggtaagggc
      121 cgatcccgag gcgcccagcg agacttgtaa gatagaatgg ctcgctgggg cgaataccgc
      181 ggtcgatttg catggcgctg agccaatcga cgcctttgca aaaaaactca ataaaaattg
      241 tctcgctgga gacgacggtg ctacgttggc agggacttta gtccccctca tcgaaaggca
      301 acgccatgga caaggatcgg atcaagggct cggtacggca agccaagggg gccctcaaag
      361 aggtcgtggg taaggtcgcc ggcgacgcca agctcgaagc agaaggcaag tcggacaagg
      421 ttgcaggtaa ggctcagaat gcagttggca gcctcaagga tgcgcttcgc ggcaagtaat
      481 ggatcgctag caaccccgca ccagctgggg cgtttctttt ttagcctgga cgatctggcc
      541 ggcaaggtat tgagtgaaaa tttctctgcg ggccttggcg gcgcatggca gatttgtcgc
      601 tgaaacccag cctcgtacct ctgcatgctt ggctcatcga ggccgggacc agcgacatag
      661 cgacggaaac gctgttcgac gggttctgcg cgcggctgac cgcggcgggc gtgccgatcg
      721 cgcgggggtt tctgtccatc gcggggctgc atccgttatg gcgggcgcat tcgctcacgt
      781 ggaatgacgg gcgaatcgtc gaggtcaccg atttcagcca cgcgatcatg gccacggcgt
      841 tatggcaggc aagtccgttc cgccacatgc tggaaaccaa gacgcggcga ttgcaccgac
      901 ggctggcggg cgacgacgcc gtgctcgact tcgaggtgct gcgcgagttc cgcgatgcag
      961 ggatgagcga atggctagcg ctcatgtatg ggttcggctg gagtgctgcg cggccggcga
     1021 caaatcagtt cggcgtcgtc ttatcgtgga ccacatccac ggccgtcgga cgcggcaatg
     1081 gctggagtcc cctggaactg gcggcgatcg aggaactgtc ggggacgctg gcgctggcgg
     1141 tcaagggcaa tgttgcacct gccgcggcgc acgacttgct cgcggcctat ctcggcagcg
     1201 atgcggccga ccgcgtcagt gccgggcaag tgcagcgcgg ctccgtcagc cggatcgctg
     1261 ctgccattct ctgtgccgac gtgcgcggct ttaccaactt cgccgaggag accgcgcccg
     1321 aggaggtcac ccggcgtctt aacgacatct tcgactgtct cggcgatccg gtgcgcgcgg
     1381 cgggcggcga aatcctcaag tttttgggcg acggcgtgct ggccgtgttc ctgccaccgg
     1441 acggggcaaa cgtggcggtc gtgacgcctg cggtgctggc ggcggcgcgc gagatactgg
     1501 cgcgggtcga ggcgctcaac gccactgaga cggcagccgg caatccggcg ctgagcttgg
     1561 acatcgcgct gcacgctggc gaagtcacgt acggcaacat aggcaccgcc gaccgcctcg
     1621 acttcacggt tatcggcccc gccgtgaatg aggcggcgcg cctcgaggga ttgtgcaaag
     1681 tgttaggaaa gccaatcctg atttccaact cgttcgcgca ggccgcggag ggcgtgcaag
     1741 agccattgtt gtcggtgggc cggcatccgc tcaggggcgt gcgcgaagca cacgaagtgt
     1801 tcaccattga cacttgacgg tgcagatagg agcccgcggt attatcaaat ttcgatccct
     1861 tcgggtccgc gctgccgatt tggcacctgc tctggccatc gtggaaggct aacgctggtc
     1921 cacgtccccg cacgacatgc gctcgcgggc cccctgtcta aaccggagca gcgtggctga
     1981 acgccccgag cagcggatta taggacgagg ctactcatgc gcgaggatat tgagtagtcg
     2041 gccgagcgga tccttgacga agaatcgctt gacaccccaa ggctcctgta cgggcccata
     2101 tacaatggca gggccagagc gccgcacgcg cagctggact tcatcgaaat tgtcgacttc
     2161 gacagacaag tcagggaccg gcgtcccgga gccgccttcg attgcaacgc taacctgcgg
     2221 atttgcgttc gacgcagccg caaacgttac gatccaccca agatccatga cgacatccat
     2281 ctccagaatc tctgcgtaga aggacttcgc cttgtccgtc tggtccgaag cgatgtttgg
     2341 cacgatacgc ttgacaacca tgagcgcctc catcctgcgc gaagcaatcg gcatattccg
     2401 cgggcattct tgcggagccc gcaagactac cgcgatccga gcgctcacta cgcggcgatt
     2461 cggatgcgct gcagcccgcc ggaggactcc atccgtcgcg gatagaacgc gggtgcatcg
     2521 atggtctcgt cgctgcgtac tcggaaccac agcgcgtaca gcgccggcac cacgagcagc
     2581 gtcggcgcgg ttgcaacgaa caggccgccc ataatggtga tggccatagg acccagaaga
     2641 tgctgccggc gagcgggatc attcccaacg ccggacgcgg aacttcgagc cgcccccccc
     2701 cttgcgatgt cgtgtatata cactacttta tggcctgtcg taggctgagg tcgcgcgacc
     2761 agctcgccgc cctccgagat cgcctgcgat gcacgtcatg aaggagcaag cccatgggta
     2821 agttcgatcg ctatgccttc agcgctcagc cctcgttcga tgaggcggcg acgcgaaagg
     2881 cgttttccaa ggccgttccg ctcaagaccc tcgtgatcta ctgctacgat ccgcgggcgg
     2941 ccgagatacc gaacgccgtc gccaagctct tcggcgacga ggtctttccc ggagacatca
     3001 ttctcgacgg ttccggaaat cgagtggcgt cgacgacgac cattttcccc gtcatcgtcg
     3061 ccggcggccg tgcggtcgac gcgcttcggt cgatcaccat cgcgcagcat ttgttcggga
     3121 ttcagaacat cgtcgtcgtt caccactccc attgcggcgg tacaagcttt acggccgatg
     3181 ggatcatcaa cgcctacgag cacgaacacc acgttgatat ctcgaagctc tacgaccgaa
     3241 gcagcatctg tatcagcgat tatgaaacgt cgttgaagca cgacacggct ctggtccgat
     3301 cgcatggcgg cacgccaaag aacgtcaacg tcttcggcta cttctacgat atcgataccg
     3361 gcgcgctcac cgaggtggtg agagacgtcc gccggacctg actggggccg catggacagt
     3421 ccatgcgcat catagcgatt tggagtgcca cgtggctacg gaacgagagg tccttgagaa
     3481 ctgtgtctgc cataggacgc ggatggcggc acgcgcggtg actcgtttat atgacgacac
     3541 gttgcgatcc gttggtcttc gcgcaacgca gcttgccgtc ttggccgccg ttggtggcga
     3601 tgaagtcgtt tcgattgccg gactggccaa attcatgggc atggatcggt cgacattgac
     3661 ccgaaacttg gctccgcttg aaagggaggg cctgatccag gtcggcgctg agggttggcg
     3721 gcgcagtcgc acgattgaaa taacaaagaa aggacgttca ccactgggcg aggcattacc
     3781 actttgggag aaggcccagg agacactgca gcgacggctg ggtatccgca gatgggccga
     3841 tatccgggcc gacttggacg atctcgtccg cggcgcataa aaatctcatt ctccacatgc
     3901 cgaagaactt gatgccgccg cgacgatcca cgaacgaaac gcccgcatgg cgtccgtctc
     3961 ggccttcgac ttgagcgacg tgagccaata actgccgatc gacactttcg tgtcgaacgg
     4021 ctggaccagg cgaccgagcg acaattcccg ctcgaacatc gcgacaggca ggagcgcgac
     4081 gccggcgcct tgcgccgccg cctcggccat cgtagcggat gaatcgaaca tcgttccctt
     4141 gatcgcggga catgcaacgc cggtggcggc gaaccagcgc ggccactcat cggcctggta
     4201 cgagcgcaat agtgcttcgt cggcgaggtc tgcgggcgcg catgattccc gaaccgatct
     4261 gcaattattg atttggcagc aatcgcactc agagcggctg ggcctcgccg ctcgcgatct
     4321 tcaccttctc cccgttgaac tttcgcacga tgaagccgtc gaagcgctcg ttgtaggtgc
     4381 ggctgtagtc acgcaagagc ccgagcgcct gctgctcgcc gacgaagagg ccgaggacgc
     4441 tgtcggagcg gtagtggacg cggcggcgtc gcggccgagc gcgatgttgg cggcgagctt
     4501 gtcgatctcg ttgccgaggg tgagaccagc gccgcgcagg gctccagcgc cgtgccgtcc
     4561 gccgtggcct cgaccgcatg cggcagtggc tccggcggtg gtcgctgtga agagtgcagg
     4621 tccgcggcga ttggtcaaat ctggccacct tccccaaggc cgcccagatt acgctgcgag
     4681 cgccggtgct gaagctctgc aggagcgaag cggcgtcgac cccgtcacgg agggcggcgg
     4741 acgcactgga tacgggagtg tagcgaaata ataccttttc ccgtcacttc gggatcttgt
     4801 agaaccgcaa aaggtcggta atccttgtgt tgcgtgctct ccaggcgagg cccgaatgac
     4861 ggatcgccgg ccgcatatcg tcgtggtcga agacgaggcc gcccagcgcc atttgctggt
     4921 cgagtacctc ggcagacaca atttccgtgt gaccggcgtc gacggcgggc cggcactgcg
     4981 caggctcgtc gagcgtgaac tgccggctgt tgtcctgctc gacgttggcc tctccggcga
     5041 ggatgggttc gtgctcgcac ggtggctgcg cgaacgcagt gggcggcttg gcatcatcat
     5101 ggtaacagct gcgtccgaca cggtcgatcg tgtcgtggga cttgagaccg gtgccgatga
     5161 c
//
LOCUS       DATLJT010001144         4111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1920546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001144 DATLJT010000000
VERSION     DATLJT010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VM910_26430"
     CDS             <1..546
                     /locus_tag="VM910_26430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66094.1"
                     /translation="FNNAGVAEIRPLLELTEDAWDRMLAVNLRAVFFVLQAAAKRMRQ
                     QTPIAGSELRGKLIQTASIASYRGGLALMAHYAASKAGVVSITRTAAQVLAPHRITSN
                     CICPGAVDTPMWRKIDAEWSELEGWQVGEAWKRRTSLIPLGRPETAEDVAGVVSFLAS
                     RDADYMTGQAVNVDGGLVMGN"
     gene            674..1147
                     /locus_tag="VM910_26435"
                     /pseudo
     CDS             674..1147
                     /locus_tag="VM910_26435"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            1136..1564
                     /locus_tag="VM910_26440"
     CDS             1136..1564
                     /locus_tag="VM910_26440"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HVI66095.1"
                     /translation="MGQKTYRVGSEPRHANIAKIAGNLMVACAIEATAEAAALLRKYR
                     MSAPDVLDVIITSLFNVPVYQGYGRAIGHRQYMPPGFDLVLGMKDVGLALKAGEQANV
                     PLPFASVLRDAFIDAIANGDAGKDWSAIARVAARKAGLED"
     gene            1617..2876
                     /locus_tag="VM910_26445"
     CDS             1617..2876
                     /locus_tag="VM910_26445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI66096.1"
                     /translation="MGSRTMLRRSFMAAGLALLTGITFGASAAIAAEPIKIGFSMPLT
                     GGLASNGKAILAAYQMWEEDINAHGGLLGRPVKLIYYDDQSNPSLVPAIYAKLFDIDK
                     VDLVFTSYGTNLSVPSMPVVIPRNYVLLGLFALAVNSEFNYPYYFSMFPAGPDAVREF
                     SRGYFEIAKEQKLQTVAIAGADSDFAKKAADGARENATKMGFKIVYDRSYPPNTVDFA
                     PIVRGIQASNPDFVYIASYPTETVGIARAANEIGLKTKLFGGGMVGLQYASIKTQLGE
                     LLNRIVDYDFYVPEPTVPFKGIEDFLARYQAKAPSIGADLLGYFIPPFAYANGQVLEQ
                     AVAAVGSLDQRKLGDYLKTHSFDTIVGKIEYASNGEWKNPRVLWVQYQNVKGSGLDQF
                     KKAGTQVIIYPKELASGELQVPYSVGR"
     gene            complement(2988..3965)
                     /locus_tag="VM910_26450"
     CDS             complement(2988..3965)
                     /locus_tag="VM910_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66097.1"
                     /translation="MKLPRRTFLHLAVGAAALPAVSRVATAQSYPSRPVRLVVGFAAG
                     STTDILARLMGQWLSERLGQQFIIENRPGAAGNIAAEAVVKAPADGYTLLMVPPAAAI
                     NATLYDKLSFNFLSDLAPVAGVVRVPNVVEVNPSVPVKTVPELVAHAKANPGALSFAS
                     AGIGTASHMAGELFKVMTGVNMVHVAYRGDGPAMTDLIGGQVQVGFATMTASIGHIRA
                     DRLRALAVTTAARSQALPDIPTVSDFVPGYEASSWFGIAAPKDTPAAIVDHLNRETNT
                     GLADPNIMGRLADMGGMMLAGSAADFGKLIADETAKWAKVIKVAGIKPQ"
BASE COUNT          773 a         1286 c         1307 g          745 t
ORIGIN      
        1 ttcaacaacg ctggcgttgc cgaaatccgg cccctgctcg agctcaccga ggacgcgtgg
       61 gaccgcatgc tggcggtcaa cctgcgcgcc gtctttttcg tgttgcaagc cgccgccaaa
      121 cgcatgcgtc agcagacgcc gattgccggt tccgagctgc gcggaaagtt gatccagacc
      181 gcatcgattg cctcctaccg aggcggcctg gcgttgatgg cgcactacgc ggccagcaaa
      241 gccggcgtcg tcagcatcac gcgcaccgcg gcgcaggtgc tggcgcccca taggatcacg
      301 tcgaattgca tctgccccgg cgcggtcgac acgccgatgt ggcggaagat cgacgccgaa
      361 tggagtgaac tcgagggctg gcaagtgggt gaagcctgga agcggcgaac ctcgcttatc
      421 cccctcggcc ggcctgagac tgccgaggac gtggctggcg tcgtgagctt tctcgccagc
      481 cgcgacgccg attacatgac gggccaggcg gtcaacgtcg acggcggcct cgtcatgggc
      541 aactgagatc gcccagccga cggacgtcgg ggtcacggcg cacgttgccg agcaaccctc
      601 atcacgttga ggagaagcgc gccgagacaa ccgaacaaca tgacgagggg cttccaacgg
      661 cccgcgcagc caaagggtgg aaacgatgga ggtcggattt ctcggcacag gccggatggg
      721 caaggccatg gcagcgaacc tgctgaaggc agggcaccac gtgcgtgcat gggacaagtc
      781 atccgagccg ttgcacgagc tcaaacgggc cggcgccgac atagccacga acgcggccga
      841 tgcttttcgg ggcgacgcgg tcgtttcgat gctgccgaac gaccaagctc tgcgcgaggt
      901 cttcatcgaa ggcgatctgc tcaaacatgg tcgcccttcg atcgtgtccg tcaacatggg
      961 aaccgtttcg cttgattgcg tcgacgcgtt ggtcgccgcg catgacgccc gcaacattcc
     1021 ctatgtcgcg gcgacggtgt tcggacggcc cgacgtcgcg gcggccgcca agctcaacat
     1081 catggcggcc ggagattcag cagcgatcga ccgcgtgcag ctgctgttcg atgccatggg
     1141 ccaaaagact tatcgcgtcg ggtccgagcc acggcacgcc aacatcgcca agattgccgg
     1201 aaatctgatg gttgcctgtg cgatcgaggc gaccgcggag gcggcggcgc tgctgcgaaa
     1261 atatcgaatg tctgcgcccg acgtactcga tgtcatcatc acctcgctgt ttaacgtgcc
     1321 ggtctatcaa ggctatggcc gcgcgatcgg ccatcggcaa tatatgccgc ccggcttcga
     1381 tctcgtgctg ggcatgaagg acgtgggcct tgcgctaaag gcgggtgagc aggcgaacgt
     1441 gccgctgccc ttcgccagcg tgctgcgcga cgcgttcatt gatgccatcg ccaacggcga
     1501 tgccggcaag gactggtcag cgattgcgcg cgtcgcagcg cgcaaagccg gtctggaaga
     1561 ctgaaatgac cgcaaagagc tcaagacgta caccgactcg gaggggagcg aaaccgatgg
     1621 gttccaggac gatgcttcga cgctcgttca tggcggccgg tctggcgcta ctgacgggca
     1681 tcacgttcgg cgcaagcgcg gcaatcgcag ccgagccgat caagatcggc ttttccatgc
     1741 ccttgaccgg cgggctcgcc tcgaacggca aggcaattct cgccgcgtat cagatgtggg
     1801 aagaggacat caacgcccac ggaggtcttc tcggccggcc cgtgaagctc atctactacg
     1861 acgatcagag caacccttcg ctcgtgccgg ccatctacgc aaaactgttc gacatcgaca
     1921 aggtcgatct ggtcttcacc agctacggca ccaacttgtc ggtgccgtcg atgccggtcg
     1981 tgatcccgcg caactatgtt ctgctgggac tgttcgcgct cgccgtgaac agcgagttca
     2041 actatccgta ttatttttcc atgttcccgg cagggccgga tgccgtgcgc gagttctcgc
     2101 gtggctattt tgaaatcgcc aaggaacaga agctgcagac ggttgccatc gccggcgcgg
     2161 attcggattt cgccaagaag gcagccgacg gcgcacgcga gaacgcaacc aagatggggt
     2221 tcaagattgt ttatgatcgg agctatccgc cgaatactgt cgatttcgct ccgatcgtgc
     2281 gcggcatcca ggcgagcaat ccggatttcg tctatatcgc gtcctacccg accgaaacgg
     2341 tcggcatcgc gcgggcggcg aatgagatcg gcctgaaaac caaactgttt ggcggcggca
     2401 tggtcgggct gcaatacgca tccatcaaga cccagctcgg cgaattgctc aatcggatcg
     2461 tcgactatga cttctacgtt cctgagccca cggtgccgtt caaggggatc gaagacttcc
     2521 ttgctcgata ccaggccaag gccccgtcaa tcggcgcaga cctgcttggc tatttcattc
     2581 cacccttcgc ctacgcgaat ggccaggtgc tggaacaggc ggtagcggcg gttggcagcc
     2641 tcgaccagcg aaagctcggg gattacctca agacgcacag cttcgatacc atcgtcggca
     2701 agatcgagta tgcgtcaaac ggtgaatgga agaacccgcg cgtgctgtgg gtgcagtatc
     2761 agaatgtcaa gggcagcggc cttgatcagt tcaagaaagc cggtacgcag gtgatcatct
     2821 atccgaagga actggcctct ggagagttgc aggtccccta ttcggtaggg cgctgacgga
     2881 ctctggcgct cgctacccgc caacgcccag gcggcaaccg ccggggctgc cccccggtag
     2941 cgctggccga acaagtcaag ggagatcccc ctcggccgat ccatcggtta ctgcggcttg
     3001 atgcccgcga ctttgatcac cttggcccac tttgcggttt cgtcggcgat gagcttgccg
     3061 aaatccgcgg ccgagcccgc gagcatcatc ccgcccatgt cggcgagccg ccccatgatg
     3121 ttgggatcgg cgaggcctgt attggtttcc ctgttgagat gatccacgat cgcggcgggc
     3181 gtatccttcg gcgcggcgat gccgaaccac gagctggcct catagcccgg tacaaaatcg
     3241 ctcacggtcg ggatgtccgg cagcgcctgc gagcgcgccg ccgtggtcac tgccagcgcc
     3301 cgcagcctgt cggcgcggat gtgtccgatc gacgcggtca tggtcgcaaa cccgacctgg
     3361 acctgcccgc cgatgagatc ggtcatcgcc ggcccgtcgc cgcggtaggc cacgtgaacc
     3421 atgttgacgc cggtcatcac cttgaacagc tcgccggcca tatggcttgc cgtcccgatg
     3481 ccggccgagg cgaagctgag cgcgcccgga ttggccttgg catgggcgac caattcggga
     3541 acggtcttca ccggaaccga tggattgact tccacgacgt tgggtacgcg aaccacgccg
     3601 gcgaccggag ccaggtcgct gaggaaattg aagctgagct tgtcatagag cgtggcgttg
     3661 atcgcggccg cgggcgggac catgagcagc gtatagccgt cggcgggcgc cttcacgacg
     3721 gcctcggcgg cgatattgcc ggcggcgccc ggccggttct cgatgatgaa ttgctggccg
     3781 agccgctcag agagccattg ccccatcagc cgcgcgagga tgtcggtcgt gctgccggcg
     3841 gcaaatccta cgaccagccg caccggccgc gacggatagc tttgtgccgt cgcgacgcgt
     3901 gagacagccg gcagcgcggc ggcgccaact gccagatgca gaaatgtccg acggggaagt
     3961 ttcacggcat cgctccctaa aaataccggc gccgaagcgg cttattccgc cttgatgttt
     4021 tccgccttga tgttcgccgc cttgaggatc ggccaccatt tctcgatctc ggcctgctgt
     4081 tgggcggcga gcgctgcggg cgtctgctgc t
//
LOCUS       DATLJT010001145         1581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_542927,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001145 DATLJT010000000
VERSION     DATLJT010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542927"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VM910_26455"
     CDS             complement(<1..531)
                     /locus_tag="VM910_26455"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66098.1"
                     /translation="MLRVGFVGMQPREAPHYKNFLRRMTELGYQESRNFIFDYIQTPN
                     VEGYERNYRELAARKVDVFLAVGNEPALRAALSAADGKPIAFLAIDFDPLTKGYVANL
                     SHPGGNVTGIFVRETELAAKRVEIAREAFPRATLVGIAFDTLSHEQGDAAAEAARKLG
                     LEPRMVEVKGERGYAAA"
     gene            complement(609..>1581)
                     /locus_tag="VM910_26460"
     CDS             complement(609..>1581)
                     /locus_tag="VM910_26460"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66099.1"
                     /translation="FITLLGGAAAAWPLAAHAQQERMRRIGVLTNLDEADPELKGWLT
                     AFQVELQKLGWAQGRNISFEYRWPGSDEQRLQSSAADLVKAAPDVIFAAATPALVALY
                     RESRSLPIVFVQVSDPVKLGFVASLARPGGNMTGFTTFEYPVGGKWLELLKDTAPGRT
                     RVAVLLDPSNSSGLAYLQGVEAAAASLGVQLTHADVHNAADIERAINDFAQQPNGALV
                     VAPTAATILHRNLIVTLAARHRLPAVYPYRFFASSGGFISYGVDLAEQYRQAAGYVDR
                     ILRGANPGELPVQLSSKFELVVNLKTAKALGLTIPEPFLQHADEVIE"
BASE COUNT          315 a          486 c          475 g          305 t
ORIGIN      
        1 tgcggccgca tagcctcgct cgcctttgac ttcgaccatg cgcggctcca agcccaactt
       61 gcgggccgcc tcggctgctg catctccttg ctcgtgtgaa agcgtgtcga aagcaattcc
      121 tacaagggtc gcgcgcggga acgcctcgcg agcgatctca acacgtttgg cggcgagctc
      181 ggtctctcgc acgaaaatgc cggtcacatt gccgccggga tgtgagagat tcgccacata
      241 gcccttcgta agaggatcga aatcaatggc caggaacgcg atcggcttac cgtccgcggc
      301 ggaaagcgcg gcacgtagtg ccggctcgtt cccaaccgct aggaacacgt caactttgcg
      361 cgccgcgagc tcgcgatagt ttctttcgta gccttcgaca ttcggtgttt ggatgtaatc
      421 gaatataaag ttgcggctct cctgataacc aagttcggtc atccgcctga gaaaattttt
      481 ataatgtgga gcctctcgcg gctgcatacc gacaaagccg acgcgcagca tcttgtgctg
      541 ctgcgggcgc gccgtgagcg gccacgcaac cgcgccgccg agcagtgtga tgaactcgcg
      601 acggctgctc attcgatcac ctcgtcggcg tgctgcagga acggctcggg gatagtgagt
      661 ccgagggctt tcgccgtctt aagattgacc accagctcga acttgctcga aagctggacc
      721 ggaagctcgc ccgggttagc gccccttagg atgcgatcga catagcctgc cgcttgtcgg
      781 tactgctcgg ccaagtcaac tccataggaa ataaagccgc cgctgctcgc gaagaaacgg
      841 taaggataga ctgccggcaa gcgatgccgc gctgccaagg tgacaatcag attgcggtga
      901 agaatggtcg ctgcggtcgg cgccacgaca agggcgccat ttggctgctg cgcgaaatca
      961 ttaatcgcgc gctcgatgtc ggcagcgttg tgcacgtcgg cgtgagtcaa ctgtaccccc
     1021 aacgacgccg ctgccgcctc gaccccttgc aagtatgcga gtcctgaaga attgctcgga
     1081 tcgagaagga cagccactcg ggttctgccg ggtgcggtat ctttgagtag ttcgagccat
     1141 ttgccaccaa ccgggtattc gaaggtagta aagcccgtca tgttgccgcc tggccgtgca
     1201 agactggcga caaagccaag cttgaccggg tccgatacct gaacgaacac gatcgggagg
     1261 gagcggctct ctcgatacag cgccaccaat gcgggcgtgg cagcggcaaa gatcacgtcg
     1321 ggcgccgctt tcaccaggtc ggcggcagag ctctggaggc gctgttcatc gctgccgggc
     1381 caacgatatt cgaacgaaat gttgcggccc tgcgcccacc ccagtttttg cagctccact
     1441 tggaatgccg tgagccatcc cttcagctcg ggatcagcct catcaaggtt cgtcaagacg
     1501 ccgatgcggc gcatccgctc ctgctgcgcg tgcgccgcaa gcggccacgc cgcggccgca
     1561 ccgccgagca gcgtgatgaa c
//
LOCUS       DATLJT010001146         1683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_769176,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001146 DATLJT010000000
VERSION     DATLJT010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769176"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VM910_26465"
     CDS             complement(<1..266)
                     /locus_tag="VM910_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66100.1"
                     /translation="MKNLGHIESLARSHTKTAIRVLAGIMREPTVQPMARVAAAKALL
                     DRGWGKPKEMHHSEVDDNKTLLKVVNEIVHVYETREQIEFNDQTP"
     gene            691..1308
                     /locus_tag="VM910_26470"
     CDS             691..1308
                     /locus_tag="VM910_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66101.1"
                     /translation="MTVISREELRTIKVALTDDRPYVDRISFALTVQLFEMRSSLREQ
                     ALVIDELDVLEGLRPRLHTKAAEQFKHVPLYPLWHKHFSAPRHAMRNIGNWWNICGTN
                     QKALDAMVRTVAADYGADPDVWQRVLPHRFMLDGYTDRATHQRLTGDWIIFGKHDGAN
                     YYLDLATHEEGTQPERLLEKLRAGSAAEFPFLFNRTSPTKGDDAV"
     gene            1545..>1683
                     /locus_tag="VM910_26475"
     CDS             1545..>1683
                     /locus_tag="VM910_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HVI66102.1"
                     /translation="MRPKKHETTGSADLFRARLDQIINMRHELVQLAGKIDWEFIDGE
                     IA"
BASE COUNT          329 a          483 c          513 g          358 t
ORIGIN      
        1 ggggtttggt cgttgaactc gatctgctcg cgggtctcat acacatgcac gatctcgttg
       61 acgaccttga gcagcgtctt gttgtcgtca acctcgctgt gatgcatttc ctttggcttg
      121 ccccagccgc gatcgagcag cgctttggcc gcggccacgc gcgccatcgg ttgcaccgta
      181 ggctccctca ttatgccagc gagcacccgg atggcagttt tggtgtgaga gcgcgcgagc
      241 gactcgatgt gtccgaggtt tttcattttc cgctcgtcat gttttgtaaa cattcgttac
      301 aatccgcatc tgcgctgaca aaccaatcaa gtcgcagccg agctcgcgct gaaggcgggt
      361 tgcgggttcg tcgcgccttc attggatcat agtacatttc atcgagcgtg gggcgtgtca
      421 tgtcgcgcca cggcgcaacc cgcgaggctt gtaactgtag ggccccgggc gcaggtggac
      481 cggggacccg ccgttcgcag agcgggcgtt acggaatatt tgggcgaggt cgcgccgatc
      541 actccgcctt gatgttagcg gcccggatca ccttgcccca tttctccgtt tcatcggccg
      601 aagtgctgcg ttgccgcgaa ttgacgcgac gtgccagttg cagaccttcg tgaaaggtcg
      661 cactgtccgg cccgaggaag gggccatgcg atgaccgtga tcagccggga ggaattacga
      721 acgatcaaag tcgcgctcac ggatgatcgg ccgtacgtgg accggatcag cttcgcattg
      781 acggtgcaac tatttgaaat gaggagcagc cttcgggagc aggcgcttgt aattgacgaa
      841 ctagacgtgc ttgagggact taggccgcgc ttgcacacca aagcggctga gcagtttaaa
      901 catgttccgc tatacccgct ctggcacaag cacttttccg caccgcgcca cgccatgcgc
      961 aacatcggca actggtggaa catctgcggg accaaccaga aggccttgga cgcgatggtc
     1021 cgcacggtcg ccgccgatta cggtgctgat ccggatgtct ggcagcgggt gttaccgcat
     1081 cgcttcatgc ttgacggcta tacggatcgc gccacccatc agcgtctcac cggcgactgg
     1141 atcatcttcg gcaagcacga cggcgcgaac tactacctcg atctcgcaac gcatgaagag
     1201 ggcacgcagc ccgagcgtct cctcgaaaag ctgcgtgctg gcagcgccgc cgagtttccg
     1261 ttcttattca accgaacatc gccgacgaag ggtgatgatg cggtctagcc cggcggggat
     1321 cgcgcaaaag gcagcccgcc ccacgagcgg acatttgacc agcgcgttta tgagtagtcg
     1381 cccgtgaaga atttctaact gactgagcga gagtcgtttt ttggcggctt tgttgacttg
     1441 agattgcaag gtttcggtgt ctgaggccct ggaagcttgc aaattcgttt tcgcgattcc
     1501 ctcgaatcga gaagcatgat tcgatcgccg catcggaggg aacgatgcgg ccgaagaagc
     1561 acgagacgac aggatcggct gatctgttcc gcgcgcggct ggaccagatc atcaacatga
     1621 ggcacgagct agtgcagctc gcgggcaaga tcgactggga gttcatcgac ggcgagatcg
     1681 cgc
//
LOCUS       DATLJT010001147         3487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1231188,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001147 DATLJT010000000
VERSION     DATLJT010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231188"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VM910_26480"
     CDS             <1..1221
                     /locus_tag="VM910_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI66103.1"
                     /translation="DLKVVQALTQAFTQNHAYIRLRPVQTDGASASADALALGKVDLA
                     VVRGDLDVPKNALAVATLRKNFAVLWAPPRRGKKAAPKITKIAQLAGHRVGVVGRTQA
                     NVNLLKIILQQYAVDPNKVEIVQFPAQESAEAIKNQKADVYLAAGPLNSKVTADAIAA
                     SAKDGGTPTFLEIDRADAIAQNHPAYEAGDIPAGTFASDRPDDDVKTISFNHHIVARK
                     GLSDVTVAAFTRQLFAVRQQLMAEFPLAAKIETPDTDKDAAIPAHPGAAAFVDGEEKS
                     FLDRYSDYIWWGLMALSAMGSMGAWFAGYLKKDERSTNSHLRERLLEMIADARKADST
                     EELDRMQGEADNILRDTLHCFEDGAIQEGALTAFNIALDQFHHAAADRRAVLMSLPQN
                     LQRAGAQFRATGTI"
     gene            1387..2961
                     /locus_tag="VM910_26485"
     CDS             1387..2961
                     /locus_tag="VM910_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVI66104.1"
                     /translation="MTLRIPRQTFRGLSRRRFLSTAAATGISAIAMPYLSRAADRPVV
                     THGVQSGDVGADSGVVWARADRPAQMLVEVSTTESFGNARALPPIAALPESDFTAKML
                     LENLPGGQDIFYRVKFRDLSHSSVESEPVIGRFRTAPSDRRDVSFVWGGDVAGQGWGI
                     NPDDGGMFTFSAMKKHRPDFFLHSGDTIYADGLIPSEVKLADGKIWKNVTIPEKAKVA
                     ETLDEFRAAHKYNFLDENVRAFNAEVPIFVQWDDHEVTNNWSASKDLPAAYKERNISL
                     LAARAARAFHEMYPMRESIVEPGRVYRTLSYGPLLDVFVLDERSYRGPNGPNLQESYG
                     PDAYFLGPDQMRWLKRDLLNSRATWKVIASDMPLSLIVYDDAPNRKGSEAFAQGDGPP
                     RGRELEIAEILRFIKAAPIHNTVWLTADVHYAAAHYYNPNKAQFQDFEPFWEFVSGPL
                     HAGTFGPNELDNSFGPEVRFIKAPGLDKQNLPPSAGMQFFGHVQIAGATGQMTVTLRD
                     RADVALWSTTLDPKQA"
     gene            complement(3011..3439)
                     /locus_tag="VM910_26490"
     CDS             complement(3011..3439)
                     /locus_tag="VM910_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66105.1"
                     /translation="MAGTSSMPALPQDVLIVEDDPIIALDLEDTVLGLGAATVRTAGH
                     VATALDMIAARAPDFALLDVGLVHENSFAIAERLTTLNIPFAFVTGYGSGKVPAVFAD
                     RPRLPKPFSTEALLVVLRRIGARDPKDSGGFGPLTNPPAF"
BASE COUNT          677 a         1139 c         1071 g          600 t
ORIGIN      
        1 gacctcaagg tggtgcaggc gctgacccag gccttcaccc agaaccacgc ctatatccgc
       61 ctccgcccgg tgcagaccga cggcgcctcc gcaagcgccg acgcactcgc gcttggcaag
      121 gtcgatctcg cggtggttcg cggcgacctc gacgtgccga aaaatgcgct agccgtcgcg
      181 acgctgcgca agaattttgc ggtgctgtgg gcccccccac gcaggggcaa gaaggcggcg
      241 ccgaagatca ccaagatcgc acagcttgcg gggcaccggg tcggcgtggt cggccgcacc
      301 caggccaacg tgaaccttct taaaattatc ctgcagcaat acgccgtcga tccgaacaag
      361 gtcgagatcg tgcagtttcc ggcgcaggaa tctgcggagg cgatcaagaa ccagaaggca
      421 gatgtctacc ttgccgcggg tccgctcaac agcaaggtca ccgcggatgc gatcgcggcc
      481 tccgccaagg acggcggcac gcccaccttc ctcgagatcg accgggcaga tgcgatcgca
      541 caaaatcatc ccgcctatga ggccggcgac attccggccg gcacctttgc gtccgaccgc
      601 cccgacgacg atgtcaagac gatcagcttc aatcaccata tcgtggcgcg caagggcttg
      661 tccgacgtga cagtcgccgc ctttacccgg caattgttcg cggtccgcca gcaattgatg
      721 gcggaatttc cgctcgccgc aaagatcgaa acgcccgaca ccgacaagga cgccgccatt
      781 cccgcacatc ccggtgccgc ggcgttcgtt gacggcgagg agaaaagctt tctcgaccgc
      841 tacagcgatt acatctggtg gggattgatg gcgctgtcgg cgatgggctc gatgggcgcc
      901 tggttcgccg gctatctgaa gaaggacgag cgcagcacca actctcacct gcgcgagcgt
      961 ctgctcgaga tgatcgcgga cgcgcgcaag gccgactcga ccgaagagct cgatcgcatg
     1021 caaggcgagg cggacaatat ccttcgcgac accctgcact gtttcgagga cggcgcgatc
     1081 caggagggcg cgttgaccgc cttcaacatc gcgctcgatc aatttcacca cgcggccgcg
     1141 gatcgcaggg ccgtgttgat gagcctgccg caaaatttgc aacgggccgg tgcgcaattc
     1201 cgcgccaccg ggacgattta atcgcgccgt cacataatct tcctatagcg tcttgccagg
     1261 gaatggcgtc cctctggatt gctccgtcgc ggaggtcttc attaggccgc gcttcgcgcg
     1321 gacccatcgg ttcctcgtaa tgacggaagg tggctcgcca tttccgacga tgcaaaggag
     1381 gcccgcatga cgcttcgcat tccccgtcaa acatttcgtg gactgtcccg ccggcgtttt
     1441 ctgtcgactg ccgctgcgac aggcatcagc gcaatcgcga tgccctatct gtcccgcgcg
     1501 gccgaccggc cggtggtgac ccacggcgtg caatccggcg acgtcggtgc cgacagtggc
     1561 gtggtctggg cgcgcgccga ccgtcccgcg cagatgctgg tcgaagtctc gacgaccgaa
     1621 tcgttcggca atgcgcgcgc gctgccgccg atcgcagcac tgcccgaaag cgacttcacc
     1681 gcaaaaatgc tgttggaaaa cctccccggc ggccaggata tcttctatcg cgtgaagttc
     1741 cgcgatctct cgcacagttc ggtcgaaagc gagcccgtga tcggtcgctt ccgcacggcg
     1801 ccgtccgaca ggcgcgacgt cagtttcgtc tggggcggcg acgttgccgg tcaaggctgg
     1861 ggcatcaacc ctgacgacgg tggcatgttc accttctcgg cgatgaagaa gcaccggccg
     1921 gatttcttcc tgcattccgg cgacacgatc tacgccgacg gactgatccc ctcggaggtc
     1981 aagctcgccg acggaaagat ctggaagaac gtcaccatcc ccgaaaaggc caaggtcgcc
     2041 gagacgctcg acgagttccg cgccgcgcac aaatacaatt tcctcgacga gaatgtgcgc
     2101 gcattcaacg ccgaagtgcc gatctttgtg cagtgggacg accacgaagt caccaacaac
     2161 tggtcggctt cgaaggacct gccggccgcc tacaaggagc gcaacatctc cctgctcgcc
     2221 gcgcgcgccg cgcgtgcctt ccatgaaatg tatccgatgc gcgaaagcat cgtcgagccg
     2281 ggccgggtct atcgcacctt gagctacggt ccgctgctcg acgtcttcgt tctggacgag
     2341 cgcagctatc gcggtcccaa tggcccgaac ctgcaggaga gctatggtcc tgatgcctat
     2401 tttctcgggc ccgaccagat gcgctggctg aagcgggacc tattgaactc gcgggcgacc
     2461 tggaaggtga tcgcctccga catgcccttg tcgctgatcg tctacgacga tgcgcccaac
     2521 agaaagggct ctgaggcctt tgcgcagggc gacggcccgc cgcgcggccg cgaacttgaa
     2581 attgcggaaa tcctgcgttt catcaaggcc gcgccgatcc acaacacggt ctggctgacc
     2641 gccgacgtgc actacgccgc agcccactac tacaatccga acaaggcgca gtttcaggat
     2701 ttcgagccgt tctgggagtt cgtctcggga ccgctgcatg ccggcacgtt cgggccgaac
     2761 gagctcgaca acagctttgg cccggaggtg cgtttcatca aggcgccggg actggacaag
     2821 cagaacctgc cgccatccgc cggcatgcag ttcttcggtc acgtgcaaat tgcgggcgca
     2881 accgggcaga tgacggtgac gctgcgtgac cgcgccgacg tcgccttatg gtcgaccacg
     2941 ctcgatccga agcaggcgta gacattcacg ctgaggcgga aagcgcagat gcgctcccta
     3001 ccctaacggg tcaaaaagcg ggtgggttgg tgagagggcc aaacccgccg ctatccttgg
     3061 ggtcccgcgc gccgatgcgc cgcaacacca caagcagcgc ctcggtcgaa aatggctttg
     3121 gcagccgcgg ccggtccgca aagacagccg gcaccttgcc gctcccatag ccggtaacga
     3181 aggcgaacgg aatgttcagc gtcgtgaggc gctctgcgat agcaaagctg ttttcgtgaa
     3241 cgagcccgac atcgagcagg gcaaaatccg gcgcacgagc ggcgatcatg tcgagcgcgg
     3301 tcgcgacatg tccggcggtc ctgacggtgg ccgccccgag accgaggacg gtgtcctcga
     3361 gatcgagcgc gatgatggga tcgtcctcga cgatcagcac gtcttgggga agagcgggca
     3421 tgctactggt tccagccatt gagctttgac cgggacagcc ctgcggccga cacctccctg
     3481 cgagcgc
//
LOCUS       DATLJT010001148         1784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1461297,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001148 DATLJT010000000
VERSION     DATLJT010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461297"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VM910_26495"
     CDS             complement(<1..432)
                     /locus_tag="VM910_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66106.1"
                     /translation="MFSVTSDVTAGATYQAAPPKPALPDPSPTPDSFAALIDGNLPAN
                     SASPPAAPDPSAPPAPQWSTDAAPPSDNGAAPDNMTSNQSPAANTSSDPAASTVPPTD
                     TKFAPGNSAGNANPAAQSGAKFAASKPAGTKPADKTTTDTGT"
     gene            796..>1784
                     /gene="mnmA"
                     /locus_tag="VM910_26500"
     CDS             796..>1784
                     /gene="mnmA"
                     /locus_tag="VM910_26500"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVI66107.1"
                     /translation="MLNSLDLEGRPEDTRIVVAMSGGVDSSTTAALLKSEGYDVIGIT
                     LQLYDHGAATHRKGACCAGQDIHDARNVAERIGIPHYVLDYEERFRESVIDTFAESYS
                     LGETPVPCIECNRAIKFRDLLKTARELGAQVLATGHYVASHRLPDGSRALTCAADADR
                     DQSYFLFATTREQLEYLRFPLGDMTKPQVRDLARRFGLTVADKHDSQDICFVPTGRYT
                     DIISRLRPNAMEPGDIVDRDGRVLGKHQGIAHFTVGQRKGLGIASGSPLYVLRLDAAN
                     RRVVVGPREGLRMDRIMLRDVNWIGDGALDAAIGNGLEIFVRVRSTRAPQPAW"
BASE COUNT          293 a          523 c          607 g          361 t
ORIGIN      
        1 cgtgcccgtg tcggtggtgg tcttgtcggc tggtttggtg ccggccggct tcgacgcagc
       61 gaacttcgcg ccgctctgtg cggccggatt ggcgttgccg gcagaattgc cgggggcaaa
      121 tttggtatcg gtcggcggta cggtgctggc tgcaggatcg ctcgaagtat tcgctgccgg
      181 agattggttg gaggtcatgt tgtcgggcgc ggcgccgttg tcgctcggcg gcgctgcgtc
      241 ggtgctccac tggggagcgg gcggtgcgga cggatccggt gccgcaggcg ggcttgcgga
      301 attggcgggc aggttgccgt caatcagcgc ggcgaagctg tcgggcgtcg gcgacgggtc
      361 gggcagagcc ggtttgggcg gcgcggcctg gtaagtcgcg cctgcggtga catctgacgt
      421 aacgctgaac acggggccgg cctcttgcgg tgaacttgtt tttgaggatt agcaaggagc
      481 gggccatcga aggtttgata gaatatgcaa tattatcaat agtttgtgga tacgaccaag
      541 cgattgccgg acgccgtttt cgtgctccct ttctgcccgc cggcaagaat tgccggttgc
      601 atagctccaa ggagcccgcc tcatgattgc ctgtcgggaa tacggcctat attaaagagc
      661 gacgtctcac cgtttccggc cgacgctctc ttcggaaccg cgggaagagg cttcagatgg
      721 gcgcaacttt cgtttggctt tccgcatgcg gaaagcgtgg atggcgtccg tggttctatc
      781 gatgatgtga aggcaatgct caacagtctc gaccttgaag gccgtcccga agatacgcga
      841 atcgtggtcg ccatgtccgg cggcgtcgac tcctcgacga cggctgcgct gttgaaatcc
      901 gaaggttacg atgtcatcgg gatcacgctg cagctttacg atcacggcgc ggcgacgcat
      961 cgcaagggcg cctgctgtgc cggccaggac attcacgacg cgcgcaacgt ggccgaacgg
     1021 atcggcatcc cgcattacgt tctggattac gaggagcgct tccgcgaatc cgtgatcgac
     1081 acctttgctg aaagctattc gttgggcgag acgccggtgc cctgcatcga gtgcaatcgc
     1141 gcgataaaat tccgtgatct gctcaagacc gcgcgcgaac tcggcgcgca ggtgcttgcc
     1201 accggccatt acgtcgcctc gcaccgtttg ccagacggat cgcgcgcact gacctgcgcg
     1261 gccgacgccg atcgcgatca gagttatttc ctgtttgcga ccacgcgcga gcagctcgaa
     1321 tatctgcgct ttccgctcgg cgacatgacc aagccgcaag tgcgcgatct tgcacggcgg
     1381 ttcgggctca ccgtcgccga caagcatgac agccaggaca tctgcttcgt accgaccggg
     1441 cgctacacgg acatcatcag ccgtttgagg ccgaacgcga tggagcccgg cgacatcgtc
     1501 gatcgcgacg gccgcgtgct cggaaagcat cagggcatcg cgcatttcac cgtcgggcag
     1561 cgcaaggggc tgggcatcgc gtcgggcagc ccgctttatg tgctgcggct cgatgctgcg
     1621 aaccgccggg tcgtggtcgg cccgcgcgag gggctgcgga tggaccgcat catgctgcgc
     1681 gacgtcaact ggatcggcga cggcgcgctc gacgctgcga tcggcaatgg gctcgagatt
     1741 ttcgttcgtg tgcgctcgac ccgtgcgccg cagccggcct ggtt
//
LOCUS       DATLJT010001149         8779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2379461,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001149 DATLJT010000000
VERSION     DATLJT010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379461"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VM910_26505"
     CDS             <1..214
                     /locus_tag="VM910_26505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66108.1"
                     /translation="PCVSYWDPPQLDKNGLRRLLSRAGKDDVAFSVASFSRTPVEIIR
                     SAIAENDAQIVPLAGSNEPAELAVSC"
     gene            208..1659
                     /locus_tag="VM910_26510"
     CDS             208..1659
                     /locus_tag="VM910_26510"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HVI66109.1"
                     /translation="MLIGQASINLTANILSALLGLLSVFVFTRLFSPHDYGTYLLGTG
                     FASVISVFLAGWFRNLVLSGHARNDGTDVRGLVASGFLLSCLTAPIAYGLGVLTGLGA
                     AAAVAAIALSIAIAFFDLTQDLIRARLRAFAVMRGTLVRSAGVLCLGVLVALVSPTGL
                     VLLLSSTAACLIAILVQSRTAWRGTMMVFDRADLAALARQGLPLTVSLTLLAVSSVAD
                     RFVIANLVGAADAGRYVAALDLVRQTLMLPAMSAAAAFFPLAVQIHARKGNAAVRSHL
                     AECVELLFSITLPAALGFAMISANVANVVLGADFREIAAQTMPIVAVAVIFQILTQQY
                     LHASFLLSGRNSFYLINPASIIVANVILSCVLVESHGTVGAAWARLGADAIGFLCALV
                     LSRSAFPVPLPAGRLALVMIAALIMALMVGALDRHLHVGDLTACIVLACTGFCSYAAL
                     CWLFDISRLRGRLKSGVALLRTKRANLTIGPSP"
     gene            1656..2486
                     /locus_tag="VM910_26515"
     CDS             1656..2486
                     /locus_tag="VM910_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI66110.1"
                     /translation="MSKSASLDLISTGPAKGRNARPFAERQSPAAHPRSLLDDLSPGD
                     ARESLLAVHELLRTELPATRLRVYEAGGGSTCFLPLDMLQRAEVTAVDIDAEQLRNNE
                     YAQEGILGDIQTYRFNPDSFDLVICYNVIEHVRDVEAALIGFFESIKRGGLILVGAPN
                     PKSLSGVVTKYTPHWFHVWFYRYVIGNSLAGQPGQPPFPTYFHPMVTPSKLEAFAEEY
                     GLEVIYRKEYESPRFPEMRARTPLFAATLDIFAVAINALLPRGADVRHGDYHIIIRRR
                     "
     gene            2836..5103
                     /locus_tag="VM910_26520"
     CDS             2836..5103
                     /locus_tag="VM910_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVI66111.1"
                     /translation="MLVYDQPLNRTRSSDRATTQPPLGFDVLALSRLLWGRRVMIVAA
                     ALLCAIVAVMIGKSLTPRYTATAQLYVDPRELQLVDRELTPRAQDVSGLSMVVESQAR
                     LITSNSVLLQVIQNTHLAKDPEFGGETRGILASLLGLLGLGSHSTTDPRHDLTPALDA
                     LSRHITIRKTERSFVVDVEVWSHEAEKAALLANAIGNAYLAESRNSQASAARRATSDL
                     SGRLKELQQKLRDAETALATYKAQNNFVGTQDTLLSDQQLSASNQRLAAAHAATLDAQ
                     ARYDQIEAGRHASSDPGAIVEALQSPTIANLRAQYAEARKKYAELTAELGPLHPSFKQ
                     MDKQVEDLRRTINEEVERFAQSAKNDLTRARDYEASLNKALETQKRQSVQLSQASVRL
                     RELERDVEANRDVYQTFLKRSRETEEQESLNTSAARIIGEATLPQRRSFPPPMSLIAM
                     IGFMLGALAAMGWIVAVDRLSHETSKPQPGDPEARSAASTAKPPAETATRREAEAEPL
                     EKPLLARLQESDVIRTLGDILGAGSTADVTRFGWPTLRPAFPMTTFLNALRDMRAAVA
                     RRSATGKTQVVAVVGVGQSMDRSVTALNFSLAAARDGAEVLMIDADRKARALSGKVHC
                     AGKSEPSRLGRLSIGSEASRAIKTANGISILPAAADSDAKATEAIRKSLAQVRTTGGY
                     DLVILDGPALPWEADDRKLLEAVAGIVAVLPLSLDINEAMEDIITSLGNAQRKLAGVI
                     LNELNAPVQRGKQYA"
     gene            5096..5920
                     /locus_tag="VM910_26525"
     CDS             5096..5920
                     /locus_tag="VM910_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HVI66112.1"
                     /translation="MLERRLNPVGRVATAGIPRITLGGLRLAVLDIEQTANFMIEMAF
                     PHRRLGRPLYLTSANGEVLSRCSTEPITERLFRSADLISADGQPLVAVSRLKSMTPLP
                     ERVATTDLFDVVARKAAAAGLTFYMLGATERENAAAASRVQTMYPTLRIIGRCHGYLR
                     GDALRAKIDEINRLAPDYLWVALGVPYEQAFVEEFAPRLYNVGLIKTAGGLFNFLSGS
                     KPRAPRWMQNVGLEWVWRIWLEPRRLFWRYLTTNPRALYLLFNGSRPTYTNNDSHQ"
     gene            5939..6955
                     /gene="galE"
                     /locus_tag="VM910_26530"
     CDS             5939..6955
                     /gene="galE"
                     /locus_tag="VM910_26530"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HVI66113.1"
                     /translation="MSDRLTILVTGGAGYIGAHACRALTAAGYRPIVYDNLSTGHRAF
                     VSAPLVVGDLRDKAALGRAFAEYDIKAVMHFAAASLVGESMVDPQKYYLNNVVGTLSL
                     LDVMRGAGCSRLVFSSTGAVYGQADSKALPESFPCVPINPYGASKWMIERLLADYRSA
                     YGFGAFCLRYFNACGADEAGDIGELRDNETHLIPRAMMTLQGHLSDFAVFGDDYDTPD
                     GTAIRDYIHVTDLAAAHVAALRLLMQGHSGGVFNLGTGTGFSVREILAAIAAGTGRAV
                     PHVIKPRRPGDPAYLVADPSAARSVLNFTPAYSDLPTIIRTAWAWHQKAHPLEARVSQ
                     SLAK"
     gene            complement(6965..7564)
                     /locus_tag="VM910_26535"
     CDS             complement(6965..7564)
                     /locus_tag="VM910_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HVI66114.1"
                     /translation="MRFPFDGLAAGLLIGTAVLYAGAASGESDGWITLLDSTNKGEWS
                     EVGKANWDMKDGALVADKLDGKDLSYLVSKNSYKDFEIRAEFWVDDAANSGIFIRCGQ
                     SDKIDSKICYEVNIFDKRPDPTYGTGAIVDVAKVDPMPKAAGKWNTYEITARGPHLTV
                     VLNGQKTADVDDSKHLSGPFALQYGSGVVKFRKVQIKPL"
     gene            7699..8226
                     /locus_tag="VM910_26540"
     CDS             7699..8226
                     /locus_tag="VM910_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66115.1"
                     /translation="MTTKRAGIIRCRKQGEHTMKAIAGFWRYLALPLSFAAMLSIGSA
                     AELNPAAVVYKLPDQIPWSPVDARGAQSAVVVGDPSKPGFYMVYNKWTKGNHFSRPHF
                     HPNDRYIVVLQGTWWVGSGPKFDPEHGTVPMPAGSFVTHYGKQVHWDGAKDEDAVLLV
                     MGEGPATSTPVTEEK"
     gene            8493..>8779
                     /locus_tag="VM910_26545"
     CDS             8493..>8779
                     /locus_tag="VM910_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HVI66116.1"
                     /translation="MPILQADRLTRIGAALLGAAGASEEEARAVAVGCVNANLAGHDS
                     HGVIAIPTYIDRIKAGHIVPGAKWTIVQESPTTTVIDGHWGFGFHVNAKAMA"
BASE COUNT         1579 a         2892 c         2647 g         1661 t
ORIGIN      
        1 accctgcgtg agctattggg atccgccgca actcgacaag aacggattgc gcaggcttct
       61 gagtcgcgcg ggcaaggacg acgtcgcctt ctcggtcgcc tccttttcgc gaacgcccgt
      121 ggagatcatc cggtcagcca tcgcggaaaa tgatgcgcag attgtgccgc ttgctggcag
      181 caacgaacct gcggagcttg ccgtatcatg ctgatcggtc aggccagcat caatctgacg
      241 gcgaacattc tctctgcgct gctggggctc ttgagcgtgt tcgtcttcac gcgactgttc
      301 tcgcctcacg attacggtac ctatctgctt ggtaccggct ttgcttccgt catcagcgtg
      361 tttcttgccg gctggttccg caacctcgtg ctgagcggac acgcgcgcaa tgacggcaca
      421 gacgttcgag gcctcgtggc ctcgggattc ctgctgtcct gtcttaccgc accgatcgcc
      481 tacgggctcg gcgttttgac cggattgggt gcggcggccg cagtcgccgc gatcgcactg
      541 tcgatcgcaa tcgctttttt cgacctcacc caggatctga tcagagcgag gctcagggcg
      601 ttcgccgtga tgcggggcac gctggttcgc tccgcgggcg tgctgtgcct tggcgttctc
      661 gtcgccctcg tcagcccgac cggtcttgtc ctgctgctgt catccacggc agcctgcctg
      721 attgccatcc tcgtgcagtc gcgcacggca tggcgcggca cgatgatggt gttcgatcgg
      781 gccgatctgg ccgcgctggc gaggcagggc ctgccgctga cggtgtcgct gaccctcctc
      841 gccgtctcca gcgtcgccga ccgctttgtg atcgccaacc tcgtcggcgc ggccgatgcc
      901 ggccgctatg tcgcagccct cgatctcgtc cgccagacgt tgatgttgcc ggcgatgagc
      961 gctgcggccg cgttctttcc gctcgcggtg cagattcatg cgaggaaagg caacgcggcc
     1021 gtccgctcgc atctggccga gtgcgtcgaa ctcctgttca gcatcacgct gccggccgcg
     1081 ctcggttttg cgatgatctc ggctaatgtc gccaatgtcg tgctcggcgc cgatttccgc
     1141 gaaatcgctg ctcaaaccat gcccattgtt gccgtcgccg tgatcttcca gatcctgacg
     1201 cagcaatatc tgcacgcaag cttcctgctc tcgggacgca actccttcta tctcatcaat
     1261 cccgcatcga tcatcgtcgc caacgtgatc ttgtcctgcg ttctggtcga aagccacggc
     1321 acagtcggcg ccgcatgggc gcgtctgggt gccgacgcga tcggtttcct ctgcgcgctt
     1381 gttctcagcc gctcggcctt tcccgtaccg cttccggccg gccggctggc gctcgtcatg
     1441 atcgccgccc tgatcatggc gctcatggtc ggtgcgctcg acaggcatct gcacgtcggc
     1501 gatttgaccg cctgcatcgt gctggcctgc accggatttt gcagctacgc cgccctctgc
     1561 tggctgttcg atatttcaag actgcgcggc cgactgaaaa gcggcgtcgc gttgcttcgg
     1621 accaagcgcg ccaacctcac gatcggaccc agcccatgag caagtcggcc agcctcgacc
     1681 tcatcagcac cgggcctgcc aaaggacgca atgcacggcc atttgccgag agacagtcgc
     1741 cggccgcgca tccgcgatca ttgctcgacg atctgtcgcc cggcgacgcg cgcgaaagcc
     1801 tgcttgccgt ccacgaactc ctcaggaccg aactgccggc aacccggctc agggtctatg
     1861 aggccggcgg cggatcgacc tgcttcctgc cgctcgacat gctgcaacgc gcagaggtca
     1921 ccgcggtcga tatcgacgcg gaacaactcc gcaacaacga atatgcgcag gaggggatcc
     1981 tcggcgatat ccagacttat cgttttaatc ccgacagttt tgacctcgtg atctgctaca
     2041 acgtcatcga acatgtccgc gacgtcgaag cggcgctgat cgggtttttc gagtcgatca
     2101 aaagaggcgg cctgatcctg gtcggcgcgc cgaacccgaa atcattgtcc ggcgtcgtca
     2161 ccaaatacac gccgcactgg ttccacgtct ggttctaccg ctacgtgatc ggcaattccc
     2221 tcgccggcca gcccggccag ccgccattcc cgacctactt ccaccccatg gtcacgccct
     2281 cgaagctgga agcctttgct gaggagtacg gcctcgaggt gatctatcgg aaagaatacg
     2341 agagtcctcg tttcccggaa atgcgcgcgc ggacgccgct gtttgcggcc acgctcgaca
     2401 ttttcgccgt tgccatcaac gcgctgctgc caaggggagc cgacgtgcgg cacggcgact
     2461 atcacatcat cattcgaagg cgctgaaccg tgaacacgag gctcgcgcaa ggcggaatga
     2521 aggaaaggcc gacgggacca ttgcgggttg gcgcgtgcct tcggccgcgc gtgttcgaag
     2581 tcgcgagccg gcttaacgag tccttaacgt cagcgacgct tctgccgcgc gccatcagag
     2641 gccgcgaggc gcggcggcgc ggcgccaaac gctcggctct aaacgctttc tttgcgattt
     2701 cggtgaatag tccttcgttg agtatggtta atttttcctg ttgcgttgag ttggcgccaa
     2761 gacgcgcccg tggcgtgacc cgaagagtaa cccctcagcc aatgcgtgcg gcggttcgga
     2821 tggaagctgg ggatcatgct cgtctatgat cagccgttaa atcggacgag gtcgagcgat
     2881 cgcgcgacga cgcagccgcc gctgggcttc gacgtattgg cgctgtcccg gctgctctgg
     2941 ggccggaggg tcatgatcgt tgccgcggcg cttctctgcg ccattgtcgc ggtgatgatc
     3001 ggcaaatcgc tcacgccgcg ctacacggcg accgcccagc tctatgtcga cccccgcgag
     3061 ttgcagcttg tagaccgcga gctcacacct cgcgctcagg acgtttcagg cctgtcgatg
     3121 gtggtcgaaa gccaggcacg gctgatcacc tcgaacagcg tgctgttgca ggtcatccag
     3181 aacacccatc tggccaagga tcccgaattc ggcggcgaaa ccagagggat tctcgcctct
     3241 ttgctcggcc tgttggggct cgggtcgcac tccacgaccg atcccaggca cgatctgacg
     3301 ccggcacttg acgcgttgag ccgtcacatc acgatccgca agaccgagcg cagcttcgtc
     3361 gtcgacgtcg aggtctggtc gcacgaggcc gagaaggcgg cgctgctcgc aaacgccatt
     3421 ggcaatgcgt atcttgcgga gtccaggaac tcgcaggcca gtgccgcgcg gcgtgccacc
     3481 agcgatctgt ccggccgcct gaaggagctg cagcaaaagc tgcgcgacgc cgagaccgcg
     3541 ttggcgactt acaaggcgca gaacaatttc gtcggtacgc aggatacgct gctgagcgac
     3601 cagcagctct ccgcctccaa ccagaggctc gccgcggcgc atgctgcgac gctcgacgcg
     3661 caggccagat atgaccagat cgaggccggt cgccacgcat caagcgatcc cggggcgatc
     3721 gtcgaagcgc tgcagtcgcc gacgattgcc aatctgcgcg cgcaatatgc ggaagcgcgc
     3781 aaaaaatatg ccgagctgac cgcagaactt ggtcccctgc atccctcgtt caagcagatg
     3841 gacaagcagg tcgaagacct caggcggacc atcaatgagg aagtcgaacg cttcgcccaa
     3901 tcggcgaaga acgacctgac ccgcgcacgg gattacgagg cgtcgttgaa caaggcgctg
     3961 gagacacaga agcgtcaaag cgtgcagctc agccaggcgt ctgtgcgcct gcgcgagctg
     4021 gagcgcgacg tcgaggcaaa ccgcgacgtc tatcaaacct tcctgaagcg ctcgcgcgag
     4081 acagaggagc aggagagtct caacacttca gcggcccgca tcatcggcga ggccacgctt
     4141 ccgcaacgcc gttccttccc gccgccgatg agcctgattg cgatgattgg cttcatgctc
     4201 ggtgcgcttg cggcaatggg ctggattgtc gccgtcgatc ggctgtcgca cgagaccagc
     4261 aagccgcagc cgggcgatcc cgaagcccgg tctgctgcgt ccacggcaaa gccgccggcc
     4321 gagacggcga cacgccgcga ggcggaggcg gagccgctcg aaaagccgct cctcgcccgg
     4381 ctccaggagt ccgacgtgat ccgtaccttg ggcgacatac tcggcgccgg ctcgaccgcg
     4441 gacgtcacgc gttttggctg gccgacgctg cgtcccgcat ttcccatgac gacgtttctc
     4501 aatgccctgc gcgacatgcg cgcagcggtc gcccggcgtt cggcaacagg caagacgcaa
     4561 gtcgtcgccg tggtcggcgt cggccagagc atggatcgca gcgtgaccgc gctgaatttt
     4621 tccctcgccg cggcgcgcga tggggccgag gtgctgatga tcgatgccga tcgcaaggct
     4681 cgcgcgcttt cgggcaaggt gcattgcgcc ggcaagagcg aaccgagccg gcttggccgg
     4741 ctgtcgatcg gcagcgaggc ctcgcgcgcg atcaaaacgg cgaacggaat ctcgattttg
     4801 ccggctgccg ccgacagcga cgccaaggca accgaagcga tccgaaaatc cctggcgcag
     4861 gtgcgcacga ccggcggcta tgatctcgtg atcctcgacg gaccggcgct gccgtgggaa
     4921 gccgacgacc gcaagctgct cgaagcggtc gccggcatcg tcgcggtgct gccgctcagc
     4981 ctcgatatca acgaagccat ggaagacatc atcacatctc ttggtaatgc gcagcgcaag
     5041 cttgcgggcg tcattctgaa cgagttgaac gcgcccgtgc agcgaggcaa acaatatgct
     5101 tgagcgccgc ctaaatccgg tcgggcgggt cgcgaccgcc ggcattcccc gtatcacgct
     5161 cgggggcttg cggcttgccg tgctcgatat cgagcagacc gcgaatttca tgatcgagat
     5221 ggcattccca catcgccgcc tcggccgccc gctctatttg acgtccgcca acggcgaagt
     5281 gctttcgcgc tgttcgacgg aaccgatcac cgaacggctg ttccgctcgg ccgacctcat
     5341 cagcgcggac ggccagccgc tggttgcggt ttcgcgcctg aaatcgatga cgccgctgcc
     5401 ggagcgcgtc gcgacaaccg atctgtttga tgtcgtcgcg cgcaaggctg ccgcggcggg
     5461 attgaccttc tacatgttgg gtgcgaccga aagggaaaac gcggccgccg cgtcgcgcgt
     5521 gcagacgatg tatccaacct tgcgcatcat cgggcgctgc cacggttatc tcaggggcga
     5581 cgcgttgcgc gccaagatcg acgaaatcaa tcggcttgcg ccggactatc tctgggtggc
     5641 gctcggcgtt ccctatgaac aggccttcgt cgaagagttc gcgccgcgtc tttacaatgt
     5701 cggcctcatc aagaccgcag gcggcctttt caacttcctg tcaggaagca aaccgcgcgc
     5761 accgcgatgg atgcagaatg taggcctgga atgggtctgg cgcatctggc tggaaccgcg
     5821 ccggctgttc tggcgctact tgaccaccaa tccgcgcgcg ctctatctgc tgttcaacgg
     5881 cagccgcccc acttatacga ataacgacag ccaccaatag taacagggac cgagcaaaat
     5941 gagcgatcgg ctgaccatcc tggtcacagg cggtgcgggc tatatcggcg cgcatgcctg
     6001 tcgcgcactg acagcagccg gctaccggcc catcgtctac gacaatcttt ccaccggcca
     6061 tcgcgcgttc gtcagtgctc ccctcgtggt cggcgatctc cgcgacaagg ctgcacttgg
     6121 gcgcgccttc gccgaatacg acatcaaggc ggtgatgcat ttcgcagccg ccagcctggt
     6181 cggcgaatcc atggtcgacc ctcaaaaata ctacctcaac aacgttgtcg gcaccctgtc
     6241 cttgctggat gtcatgcgcg gcgccggctg cagccggctg gtgttttcct cgaccggcgc
     6301 ggtctatggc caggccgaca gcaaggcgct gcccgagagc ttcccttgcg tgccgatcaa
     6361 cccgtatggc gcatcgaaat ggatgatcga gcgcctgctt gccgactacc gcagtgccta
     6421 cggctttggg gcattctgcc tgcgctattt caacgcctgc ggcgcggatg aagctggcga
     6481 catcggcgaa ttgcgcgaca acgagaccca tctcatcccg cgcgccatga tgacgctgca
     6541 gggccatctt tcagatttcg cggttttcgg cgacgactac gatacacccg acggcaccgc
     6601 gatccgcgac tatattcatg tcacggacct cgcggcggcg catgtcgccg cactccggct
     6661 cctgatgcag ggacattcgg gcggcgtatt caatctcggc accggcacgg gcttttcggt
     6721 gcgcgagatt cttgccgcga ttgccgcagg aaccggtcgc gcagttcccc acgtcatcaa
     6781 gccgcgtcga ccgggcgatc ctgcctatct cgtcgccgac ccgtccgccg cgcgttcggt
     6841 gctgaacttt acgcctgcgt attccgatct tccgaccatc atccgcaccg cctgggcctg
     6901 gcaccaaaag gcccacccgc tagaggcgcg ggtgagccaa tccctggcca aatagccgat
     6961 caacctatag cggcttgatc tgcactttcc ggaatttcac cacgccggag ccgtattgca
     7021 gcgcaaacgg accgctgaga tgcttggagt catcgacatc ggccgtcttc tgtccgttga
     7081 gcaccacagt gagatgcggt cccctcgcag tgatctcata agtgttccac ttgcccgccg
     7141 ccttcggcat cggatcgacc ttggcgacat cgacgatcgc gccggtgccg taagtcggat
     7201 ccggccgctt gtcgaaaatg ttgacctcat agcatatctt tgaatcaatc ttgtctgact
     7261 ggccgcaacg gatgaagata ccgctgttgg cggcgtcatc gacccaaaac tccgcacgga
     7321 tctcgaaatc cttataggag ttcttgctga ccaggtaaga gaggtccttg ccgtcgagct
     7381 tgtcggccac tagcgcgccg tccttcatat cccaattggc tttgccgacc tcgctccact
     7441 ctcccttgtt ggtcgaatcg agcagcgtga tccagccgtc gctctcgccc gatgccgcgc
     7501 ctgcatagag cacggctgtc ccgatcaaga gtccagcggc cagcccgtcg aagggaaatc
     7561 gcattctcaa gtcctcccgt ttattgtttt gaaccctggc aaagaagacc caataccaac
     7621 gcgatcttca tgccgcaact gcgtcgcgcg catagacgag cgcccgcgtc ttgcgccagc
     7681 acaaggcgag cggatagcat gaccacaaag cgcgccggca tcatccgctg cagaaaacaa
     7741 ggggagcaca ccatgaaggc catcgcggga ttctggcgtt atcttgcact gccgctgtcg
     7801 ttcgccgcca tgttgagcat cggctcggcc gccgaactca atcccgccgc cgtggtgtat
     7861 aaattgccgg accagattcc ctggagtccg gtcgacgcac gcggcgcgca aagcgcagtc
     7921 gtcgtcggcg atccttcaaa gcccggcttc tacatggtct acaacaagtg gaccaagggc
     7981 aatcatttca gccgcccgca cttccacccg aacgaccgct acattgtcgt gctgcagggc
     8041 acctggtggg tcggatcggg gccgaaattc gatccggagc acggcacggt cccgatgcct
     8101 gccggaagtt ttgtcacgca ttacggcaag caggtgcatt gggacggcgc aaaggacgag
     8161 gacgccgtcc tcctggtcat gggcgaagga ccggcgacat cgacgccggt cacggaggag
     8221 aagtaaggcc gcgcccggcc ggtcaggcac aggcgccgct cggcgcgccg gtgaagaatc
     8281 ctccaggccg tccgccccgg ccgcagcgga gccgaagccg atttgggctg cagttgcagc
     8341 gggcgcgtcg tgaccgggtg accccggccg gtatcgcatt gcacccatga atcccgcgcc
     8401 gggcattgcc atctgcggac ggctttgaga ccatgcgacg atcagaacaa tcaagatccg
     8461 ccttccagcg cgggcattcg ccgaggaaac ccatgccgat ccttcaggcc gatcgtctca
     8521 cccgcatcgg cgccgccctg ctcggggcag cgggcgcgtc cgaagaagag gcccgggcgg
     8581 tggcggtcgg ctgcgtgaat gccaacctcg caggccacga ttcccatggc gtcatcgcaa
     8641 tcccgaccta tatcgaccgc atcaaggccg gtcatatcgt gcccggcgcg aaatggacca
     8701 ttgtgcagga gtcgccgacc accaccgtga tcgacggtca ttggggcttt ggcttccatg
     8761 tcaacgccaa ggcgatggc
//
LOCUS       DATLJT010001150         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4217454,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001150 DATLJT010000000
VERSION     DATLJT010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217454"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..550
                     /locus_tag="VM910_26550"
                     /pseudo
     CDS             386..550
                     /locus_tag="VM910_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(800..1030)
                     /locus_tag="VM910_26555"
                     /pseudo
     CDS             complement(800..1030)
                     /locus_tag="VM910_26555"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(943..2013)
                     /locus_tag="VM910_26560"
     CDS             complement(943..2013)
                     /locus_tag="VM910_26560"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVI66117.1"
                     /translation="MNNASVSISPPSSAAAKSVTPSTAARTKLLLEGPILPTLLRLAA
                     PNVLNLLAFAGVITFDGFFLGRIGTNALAGASLAFPWVMLVLQTTNSGMGTGVSSAVS
                     RALGAGRRDRADDLVFHAFLLALALAAVFSTVMLLGAPFMFRWMGGRDEMLSDALAYA
                     NVALGGAVVICVSNLLGNAVRGTGNMSFPAAVLIGCVVVHIAISPVLIFGVGPLPALG
                     PAGAGWGLVIPFAGGSVLMIWYLRSRAIVGLNFRGVTPRWELFADILKVGVPGLINIA
                     ITNLSVVVLTGIAGQLGRDTALGYAMGARLEYILQPIAFGFGTAIVAMVGTNWARGSI
                     AAPVRSHGPGRLRSRSCAERSA"
BASE COUNT          579 a          898 c          885 g          472 t
ORIGIN      
        1 tttaggcacc ggaccgagac tgcttaaatt ttagcgggac acttcaccgg gacacttcga
       61 tttttgctgc aatgggaagt gtcccgctca tccttaatgt attgaaatcg taaatctttt
      121 atgcgggaca cttcccggga cacttcagac cctagggggg tccgggggga ttgaagtgta
      181 acgctctgcc tgtgtcttag cgtgaggaat ctccacagcc ggctactgtg ttgcagcaag
      241 aaactgagat agccccagaa ccggccggac acggtcaccc acttagcaag tgtgaggcat
      301 acaaccgtgt ctatgagcaa tcctacggat cgagttgaga tcatcacatc ggttcagcgc
      361 cgccggcgct cgatagcggc tgaggaaggt tcggatcgtc gaagagacct ttgagccggg
      421 cataacggta agcttggtgg ctcgccgact tggcgttgcc ccgaaccagc tgctcacgtg
      481 gcgccggctt gtggtggagg gggcgctgac ggctgccggc agcggcgaac aggtcgtgcc
      541 ggcctcggat tatcgggctt tgcagagccc aggtgagcga gctgcgttcg gaggtcgttg
      601 ccagcgcggc attgggtgtc ttcacgctga acaccgcgtt gaccagcagc gctgcgtaaa
      661 ggcagaatcc cgcggcgacc gccgcaaaga agccggcgac gccgaggtcg aaccagtaga
      721 tcgcgacgag cccgccgctc gcgctcacga ccatgcgtac ggcattggcg ttcatggccg
      781 cgacggcgcg gccgacaccc agggtgacgt agaaaagccc gaggcccagc ccgaacaaga
      841 gataggccgg gccgacgatg cgcaggtaga gcgtgccgag acgcgccacg tcggcatcgt
      901 cgctgaatag gccgatccac aggccgggat ggaatgcgac gatcaggccg atcgttccgc
      961 acaagaccgc gatcgtagtc gccccggtcc atgcgatctc acgggcgcgg cgatactgcc
     1021 gcgcgcccag ttcgtgccga ccatcgcgac gatcgcggtg ccgaagccga acgcgatcgg
     1081 ctgcaggatg tattcgagac gcgcgcccat ggcgtagccg agggcggtgt cgcgccccaa
     1141 ctgtccggcg attcccgtca gcacgacgac cgacagattg gtgatggcga tgttgatcaa
     1201 gccgggcacg cccaccttga ggatgtcggc gaacagctcc cagcgcggcg tcaccccgcg
     1261 gaagttgagc ccgacgatcg cgcgggaacg caagtaccag atcatgagga cgctgccgcc
     1321 ggcgaacgga atcaccagtc cccagccggc gccggccgga ccgagcgccg gcaatggtcc
     1381 gacgccgaag atcagcacgg gcgaaatggc gatgtgcacg acaacgcagc cgatcagcac
     1441 ggccgcgggg aagctcatat tgccggtgcc gcgcaccgcg ttgccgagca gattgctcac
     1501 gcagatgacg acggcgccgc ccagcgccac gttcgcgtag gcgagcgcgt ccgacagcat
     1561 ttcgtcccgt ccgcccatcc agcggaacat gaaaggcgcg cccaacagca tcacggttga
     1621 gaagacggcg gcgagcgcga gcgccagcag aaatgcgtgg aagacgagat cgtcggcacg
     1681 gtcgcggcgt cccgcgccga gcgcccgcga gaccgcggac gagacgccgg tgcccatgcc
     1741 gctgttggtc gtctgcagca ccagcatcac ccagggaaac gccagcgagg cgccggcgag
     1801 cgcattggtt ccgatgcggc cgagaaaaaa tccgtcgaac gtgatcacgc ccgcgaacgc
     1861 gagcaagttg aggacgtttg gcgctgccag gcgcagcagc gtgggcaaaa tcggaccctc
     1921 gagcaggagc ttggtacgcg ctgccgtgga cggcgtaacg ctcttggcgg ctgccgacga
     1981 tggcggagaa atcgagacgc ttgcgttgtt cataggcaac tcctgctccc tcggggcccc
     2041 gcggcgcttc gagtgcgcct tattttgcat atgtatcatc gaaatgatta tatgcaacac
     2101 atgtatcaaa aataattcaa tgcgtgaaat caatcactta gacgagggcc tgctctggtt
     2161 ttgcgatcgt cgacctgcgc acgcgtaagg tcgagtagct tgtcggccaa agccgttcgc
     2221 tcctcgccca taaccgctcg caccgccccg ccgcctggag aagttcttgc tcgaccacgt
     2281 cgggatgact gcgcgctaag gcgttgcgcc cctcacgcca ccgccgctgc cgctcagcgt
     2341 cagacccatc actcaatcgt cgccggtaaa ccttgtaggc gtcggtcacc tcgccagcgt
     2401 ccatccgcgc ctgcagcggt tcggcagatc atcaaacgaa acccaaaccc cgccaggaga
     2461 acgcgcaaag taattgcgcc cacagtagga attctcatcg caggcgcgca gccacgggga
     2521 gcaccggaag ggtttcgaca gcgccccacc tagattcaga acgattcagg cctgcgcgcc
     2581 tcaatggttt tgtcgaggga aaaaatctcg ctgtcattcc aggtggcttc gaatcgaccg
     2641 ccgacaatct ggcggagcga gcggcagcat ccggttactg aaacgcgggc cgcctccgtg
     2701 agcgcgcccc tacttgacgg caacgagctt cggccagtcc tccatttgca cgcgcgccac
     2761 gagccgatac ttgccgtcct cgggcttgaa aatcgatgct tcgccttcct tcacatcgaa
     2821 ccagtccttg ccca
//
LOCUS       DATLJT010001151         5905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3299376,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001151 DATLJT010000000
VERSION     DATLJT010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299376"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VM910_26565"
     CDS             complement(<1..809)
                     /locus_tag="VM910_26565"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HVI66118.1"
                     /translation="MTATEPIFIFFVTGEPSGDALGGKLIAALRQRTGGALRIAGIGG
                     ERMQDEGLDSLVPLDDLAVMGVAEVLPRAPLILRRVSETVAAIRALRPDAVVTIDSSG
                     FSWRVAHRLRRCGETLPLIHYVAPMVWAWRAGRARRMARWYDHLMTLLPFEPPYFERV
                     GLSCCYVGHPVLESGADRGDGARFRETHGVGEDELLITTLPGSRSAEVARLLPIFGAA
                     LKRLESLIGRFRVVVPTVETVAALVSKAVRDWPGPPIIVQRADEKYDAFAA"
     gene            947..1408
                     /locus_tag="VM910_26570"
     CDS             947..1408
                     /locus_tag="VM910_26570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66119.1"
                     /translation="MPDRRSIGATLLTWFFDLDQHAARPFAAECRAPPQEFVGPFYGF
                     DAKDEALLNDDCLADIKGTDRPRDAQPTLDIRHGLQIRMDLAERTFGDAPPIEKLIDA
                     QHPKTLLLELAYDGRQQAVIAKGTVTDAGEQLGRPPIRAQCDQRWPPDTAG"
     gene            complement(1614..2414)
                     /gene="lpxA"
                     /locus_tag="VM910_26575"
     CDS             complement(1614..2414)
                     /gene="lpxA"
                     /locus_tag="VM910_26575"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HVI66120.1"
                     /translation="MPQIHPTAIVAPGAMLAQDVVVGPYCIVGEHVTLDAGVMLCSHV
                     VVDGRTTIGAGTRIFPFASIGLEPQDLKYRGEESALVIGCNNRIREYVTMNRGTLGGG
                     MITRVGDSCLFMVGVHVAHDCRIADHVIMANNATLAGHVVIDDYAVLGGLCAIHQYVR
                     IGKHAMIGGMSGVERDVIPYGQVMGDRARLCGLNIIGMQRRGFSREDIQGLRSAYQFL
                     FSADGTLSDRVNETAERFSGIAPVDDILDFIRADSSRAICQPKGANDR"
     gene            complement(2425..2895)
                     /gene="fabZ"
                     /locus_tag="VM910_26580"
     CDS             complement(2425..2895)
                     /gene="fabZ"
                     /locus_tag="VM910_26580"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HVI66121.1"
                     /translation="MIDLACTEEQITRVDVQRIMEMIPHRHPFLMIDKVVDMVANQRA
                     TGIKSVSINENYFQGHFPARPVMPGVLIIEAMAQTAAVLVVHTLGPESEGKLVYFMSV
                     DNARFRRPVFPGDRLDVHVTKQRHRGNVWKFEGRAKVGDNLMAEAVFAAMILNE"
     gene            complement(2892..3917)
                     /gene="lpxD"
                     /locus_tag="VM910_26585"
     CDS             complement(2892..3917)
                     /gene="lpxD"
                     /locus_tag="VM910_26585"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVI66122.1"
                     /translation="MADPRFFDRAGPFTLEALAALSGARVLDRPGSARLYRDVGPLET
                     AGPEDVSFLENRKYLETFIHSRAGAAFVDEHVAGRAPPGMALLISDEPYRAYALAAQA
                     FYPAQPIIPRRAPSAVIDPTAIVAEDCDVGPNAVIEQGVRLGRRCRVGANTVIAAGVE
                     VGDDCRIGPNVTLSHCVIGSRVVLHPGVRIGQEGFGFAPDPAGPIKVPQLGRVLIGDD
                     VDIGANTTIDRGSGPDTVIGPGSMIDNLVQIGHNVVLGRCCILAGQVGISGSTRLDDF
                     VMVGGQGGLAGHLHIGSGVRIAAKSGIMRDIPAGETVCGSPAVPLTEFMRQTAVLQRL
                     ARKKDKS"
     gene            complement(3949..4668)
                     /locus_tag="VM910_26590"
     CDS             complement(3949..4668)
                     /locus_tag="VM910_26590"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HVI66123.1"
                     /translation="MLLWYSRFSALLLLFLCFLAGSAVAQAPPVPSPPTPAPPPPTQA
                     PPPPIALPTAQNLTVMVVDVQALLQNSKSAKMVRGQIEQKRGEYTKEISHQEESLRAE
                     RDALQRQQASLSPEALNQKGRDFQQKVNDLERNVQGKRQALEKSNGDALQKIQEEMLK
                     IIADIAKQRKANLVFQRTDLVLFDQAFDVTDEVLQKLDEQMPTLTVNFVAPVPSSASA
                     SPPAPAAPSGPAAPNPKPKKK"
     gene            complement(4668..>5905)
                     /gene="bamA"
                     /locus_tag="VM910_26595"
     CDS             complement(4668..>5905)
                     /gene="bamA"
                     /locus_tag="VM910_26595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HVI66124.1"
                     /translation="RREFQLVEGDAFNTTKMQRSQQRIKNLGFFKKVEVTNAQGSAPD
                     KTVVTAEVEEQSTGELSLGLGFSTTDGPLADINIRERNFLGRGQDLRIGAVVSLRSQQ
                     VDLSFTEPYFLDKNIAAGVDIFEVKTSPTANFFSGVTPPYQQFSYGGALRAGYQITDN
                     LRQTFKYTARSDDIINLQNSTSLFIVLESGQHLTSEIGQVLLYDRRDDRLEPTSGYYA
                     SIGNDFAGVGFGVDYVRNKANFGYYYSVAPEWVLSLTGEVGDIFGWNGQNVLLQDRFF
                     VGGDNLRGFQSAGIGPRDSVSGDALGGQKYYVGSVTLGVPLGLPKELGLSGRVFTDFG
                     TLYQIEPTNIVLTPAQLAQTGGVQPQVEQSPATRVSAGVGVSWKSPVGPIRLDLAYPI
                     RKEGFDKTQIFRVSFGTKF"
BASE COUNT         1185 a         1902 c         1688 g         1130 t
ORIGIN      
        1 cttgcagcaa aggcatcgta tttctcatcg gctcgctgca ctatgatcgg aggtcccggc
       61 cagtcgcgca cggccttaga gaccagcgcc gcaactgtct caacggtcgg tacaaccaca
      121 cggaagcggc cgatcaggct ctccaagcgt ttcagcgccg cgccgaaaat cggcaacaag
      181 cgggctacct cggcactcct gctgcccggc agcgtcgtga ttaacagctc atcctctccg
      241 accccgtgtg tttcccggaa tcgggcaccg tcaccccggt cggcaccgct ttcgagcacc
      301 gggtggccga catagcagca tgacagtccg acccgctcga aatagggcgg ttcaaacggc
      361 aataaggtca tgagatggtc gtaccagcgt gccatgcgtc gggcccgccc ggcgcgccac
      421 gcccagacca tcggggcgac gtaatgaata agtggcaagg tctcgccgca gcggcgcagt
      481 ctgtgcgcga cccgccaact gaaccccgag ctgtcgattg tcacgacggc gtcgggtcga
      541 agcgctcgga ttgccgccac ggtttcgctt acccgccgca agataagggg cgcccgcggc
      601 agcacttcag caactcccat caccgcgaga tcatcgagcg gtacaagact atcgagaccc
      661 tcgtcttgca tgcgctcgcc gccaataccg gcgatccgga gggctccgcc agtgcgctgc
      721 ctcagcgccg cgatcagctt acctccgagc gcgtcgccgg acggttcacc ggtaacaaaa
      781 aaaatgaaga tcggttccgt cgcagtcacg atctgcggat gccgaccacg aacagcccag
      841 cccgatcagc tgtgcggata acttcgtccc gatcaagtac gatcgttgcc tcagcctcga
      901 tcgcgatccc tagcaatcca gtttccgccg caagcacgac ggtgcgatgc ccgatcgtcg
      961 gtcgatcggc gcgacgctcc tgacctggtt tttcgacctt gaccagcacg ccgcccggcc
     1021 cttcgcggcg gagtgccgcg caccgccgca agagttcgtc ggtcccttct acggcttcga
     1081 cgccaaggac gaggccctgt tgaacgacga ctgcctggcc gatatcaagg gcaccgatcg
     1141 cccgcgcgat gcgcagccca cgctcgatat ccgccacgga ttgcagatcc ggatggatct
     1201 tgccgagcgg accttcggcg atgccccccc catcgagaag ctgatcgacg cccagcaccc
     1261 gaaaaccctc ttgctcgagc tcgcttacga cggccgacag caggctgtca tcgccaaggg
     1321 cacggtaacc gatgcgggcg aacaacttgg ccgcccgcca atccgggcgc agtgtgacca
     1381 gcgatggccg ccggacactg ccggctaaga cgagttccgt cacgtggttt tcgcgtaaca
     1441 acgaaagacc tgtgaccgcc gcgcccatgc gacaccaggc atgcggtacg ccgcgcactg
     1501 tttccggctc ggccgcacct tccaaggcta agacaaagac atcacgtccg gaggctcggc
     1561 aagattcgac gagacgtcgc ggcagctggc cgcccccggc cacgataccg agcctacctg
     1621 tcatttgccc ctttcggctg acagatggcg cggctcgagt ccgcgcggat gaagtcaagg
     1681 atgtcgtcga caggtgcgat accactgaag cgctcggcag tctcgtttac acgatcgctc
     1741 agggtgccat ccgcggagaa caagaattgg taggcgctcc ggagcccttg aatatcctcg
     1801 cgactgaagc cgcggcgttg catgccgata atgttcagcc cgcaaagcct cgcccggtcc
     1861 cccatcacct ggccgtatgg aatgacatcg cgctcaaccc ccgacatgcc gccgatcatc
     1921 gcatgcttgc cgatccggac atactgatgg atggcgcaaa gcccgccgag aacagcatag
     1981 tcatcgatga cgacgtgccc cgcgagagtg gcattgttcg ccatgatcac atggtcggcg
     2041 atccggcagt cgtgggcgac gtgcacccca accatgaaca ggcagctgtc gccgacacgg
     2101 gtaatcatcc cgcctccgag cgtcccccgg ttcatcgtta cgtactctcg gatcctgttg
     2161 ttgcagccga tgacgagggc agactcctcc ccacgatatt tgagatcctg cggttcgaga
     2221 ccgatcgagg caaacgggaa aatacgagtg cccgccccga tcgttgtgcg gccgtcgacc
     2281 accacgtgcg agcaaagcat gaccccagcg tcgagggtga catgctcgcc gacgatgcaa
     2341 tagggcccga cgacgacatc ctgagccagc atcgcccccg gcgcgacgat ggccgtaggg
     2401 tggatctgcg gcatcgctga ttgtctattc gttcagaatc atcgccgcaa ataccgcttc
     2461 ggccatcaag ttgtccccga cctttgccct gccctcgaac ttccagacat tgccgcgatg
     2521 acgctgtttg gtgacgtgca catcgagccg gtcgccggga aacaccggac gccgaaaccg
     2581 ggcattgtcg acgctcatga aatagaccag cttgccttcc gattcgggac ccaacgtatg
     2641 cactaccagg accgcagcgg tttgtgccat cgcctcaatg atcagcactc ctggcattac
     2701 gggccgcgcc gggaaatgac cctggaagta attttcgttg atggaaacac tctttatgcc
     2761 ggtcgcccgc tggttcgcca ccatgtcaac gaccttgtcg atcattaaaa atggatggcg
     2821 atgcggaatc atttccatga tcctctgaac atcgactcgc gtaatttgtt cctcggtgca
     2881 agccagatcg atcatgactt gtctttcttc ctggcaagac gctgcagcac agccgtctgc
     2941 ctcatgaatt cagttaacgg aaccgcggga ctaccgcaca ccgtctctcc ggccggtatg
     3001 tcgcgcatga tcccgctctt agcggcaata cgcactccgc tcccaatatg caaatggccg
     3061 gctagcccgc cttgtccccc gaccataaca aaatcgtcga gcctagtgct tccggagatc
     3121 ccgacctgac cggcgaggat gcagcatcgc ccgagaacga cgttgtgtcc gatttgtacc
     3181 agattatcaa tcatcgaacc tggaccgatc acggtgtcgg gccccgaccc gcgatcgatc
     3241 gtggtgttgg caccgatgtc cacatcatcg ccgatcagga cccgcccgag ctgagggact
     3301 ttgatcggac ccgccggatc gggcgcgaaa ccgaaccctt cctggccgat ccgcacaccc
     3361 gggtggagca cgacgcgcga tccgatcacg caatgactga gtgtgacatt agggccgatt
     3421 cggcaatcgt cgccgacttc caccccggcg gcgattaccg tgtttgcccc gacccggcag
     3481 cgccgcccta gccgcacacc ttgctcgatc acggcatttg gtccgacatc gcaatcctcg
     3541 gccacgattg ccgtcggatc gatcaccgcc gatggtgcac ggcgaggaat aatcggctgc
     3601 gcagggtaga aggcctgagc ggcgagggca taagcccggt aaggttcgtc gctgataagc
     3661 agcgccatcc cgggcggtgc gcgcccggcg acatgctcat cgacgaacgc cgctcccgct
     3721 cgagagtgaa tgaacgtctc taaatatttc cgattttcga gaaagctcac gtcctctggg
     3781 ccggccgttt cgagcggacc cacatcgcga tatagccgcg cactacccgg ccgatccagc
     3841 acccgcgcgc cgctcagcgc cgcaagtgct tcgagcgtga agggcccggc tcgatcgaag
     3901 aagcgcggat cggccatctg tcctccgccg gatcagtccg gccctgtatc acttcttctt
     3961 gggcttgggg ttgggggccg ccgggccgct cggcgcagca ggagccgggg gtgaggcact
     4021 cgcgctagaa ggcactggag cgacgaagtt aaccgtgagg gtcggcatct gctcgtccag
     4081 cttttgcagc acctcgtccg tcacatcgaa cgcctggtcg aaaagcacga gatcggtccg
     4141 ctgaaacacc aggttggcct ttcgctgttt ggcaatatca gcgatgattt taagcatctc
     4201 ctcctgaatt ttttgaagcg catcgccgtt tgacttctcg agagcttgcc gtttgccttg
     4261 aacattccgc tcaagatcat tgactttctg ttgaaaatcg cgccctttct gattcaacgc
     4321 ttccggtgaa agcgacgctt gctgccgctg cagtgcgtca cgttcggcgc gcagcgactc
     4381 ctcctggtgc gaaatctcct tagtgtattc gccgcgcttt tgttcgatct ggccacgaac
     4441 catcttggcc gacttcgaat tttgcaagag cgcctgcaca tcgacgacca tcaccgtcag
     4501 gttctgagcc gtgggtaacg ctattggggg cggcggagcc tgagtcggcg gtggtggggc
     4561 cggcgttggc ggcgagggca ccggaggcgc ttgcgccacg gcgctgccgg cgagaaagca
     4621 caggaatagc aacaacagcg ccgagaagcg agaataccac agcaacatca gaacttcgtc
     4681 ccgaagctga ctctgaaaat ttgggttttg tcgaagcctt ccttccggat cgggtaggcg
     4741 agatccagtc ggataggacc aaccggggac ttccacgaca ccccgacacc ggcactcacg
     4801 cgagttgccg ggctctgttc gacttgcggc tggacgcccc ctgtttgcgc cagctgggca
     4861 ggagtgagca cgatgtttgt cggttcgatt tggtagagtg tgccgaaatc ggtaaagaca
     4921 cgtccgctca atccgagctc cttgggtagc ccgagcggca ccccgagggt gacggaaccg
     4981 acataatatt tttgcccgcc gagcgcgtcg ccgctgaccg agtcgcgggg cccgatgccg
     5041 gcgctttgaa agccgcgcag attgtcgccg ccgacaaaga agcgatcctg aaggagcacg
     5101 ttttggccgt tccaaccgaa gatgtcgcct acttctccgg tcaggctcag cacccattcc
     5161 ggtgccaccg agtagtaata accaaagttc gctttattgc gcacatagtc cacgccaaag
     5221 ccgacgccag caaagtcatt gccaatcgac gcgtaatagc cgctcgtcgg ctcgagccga
     5281 tcgtcccggc gatcgtatag caggacctgg ccaatctccg aggtcaggtg ctgccccgat
     5341 tcgagcacga tgaaaagcga tgtgctgttc tgaaggttta taatatcgtc ggatcgtgcc
     5401 gtgtatttaa atgtctggcg caggttgtcc gtgatctggt atccggcccg aagcgccccg
     5461 ccgtaggaga attgctggta tggaggcgtc accccgctaa agaaattcgc tgtcgggcta
     5521 gtcttgacct caaaaatatc aacgccggcc gcgatattct tgtccaagaa atacggctct
     5581 gtgaagctaa gatcgacctg ctgagaccgt aaagaaacta cggccccgat tcgcagatct
     5641 tggccgcgtc cgagaaaatt ccgctcgcga atgttgatat cggcgagcgg accgtcggtg
     5701 gtcgaaaagc cgaggcccaa ggagagttcc cccgtcgatt gctcctcaac ctcggccgtc
     5761 accaccgtct tgtccggcgc cgatccctgg gcatttgtca cctcaacctt cttaaagaag
     5821 ccgaggttct tgatgcgctg ctgcgagcgt tgcatcttgg tagtgttgaa ggcatcgccc
     5881 tcgaccagct ggaactcgcg gcgta
//
LOCUS       DATLJT010001152         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1691476,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001152 DATLJT010000000
VERSION     DATLJT010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691476"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VM910_26600"
     CDS             complement(<1..300)
                     /locus_tag="VM910_26600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66125.1"
                     /translation="MANSLRPVTVAPKGMGLYDFVALQEGFFAAEGLEVDFDWKTFRG
                     TQSSWKNLDYFQRPQDRPYTQDKREVIQGACVWGTICNASAGMGKMVPDAYGVSPW"
     gene            512..1537
                     /locus_tag="VM910_26605"
     CDS             512..1537
                     /locus_tag="VM910_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI66126.1"
                     /translation="MESSRAPLLVDFALQGGGAHGAFTWGVLDRLLQETWLRIDGISG
                     TSAGAMNAAVLVDGYADNGVDGARAALENFWRRVARAALLSPLRRTPLDVLLGRWTLD
                     HSPVFVAMDLMARLFSPYDLSPGGANPLRNILAETVDFDRLAQASIKLFVTATNVRTG
                     RGRVFRNSEITPDVLLASACLPTLFQAIEIDGESYWDGGYSGNPTITPLVRECRSQDT
                     ILVQINPVERPGLPRSARDILNRLNEVSFNAVLLKELRMIALLRQVAQPDNSESAKWA
                     DMRIHRISSDVMVELGYSSKLNAEWEFLCMLRDEGRRAGDAFLSAHHDDLGRRSTFDL
                     DELLKGV"
     gene            1542..2506
                     /locus_tag="VM910_26610"
                     /pseudo
     CDS             1542..2506
                     /locus_tag="VM910_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384392.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
BASE COUNT          387 a          823 c          785 g          511 t
ORIGIN      
        1 ccaaggcgag acgccgtagg cgtcgggcac catcttgccc atgccggcgc tggcgttgca
       61 gatcgtgccc cagacgcagg cgccctggat cacctcgcgc ttgtcctgcg tgtaggggcg
      121 gtcctgcgga cgttggaaat aatcgagatt cttccaactc gactgcgtgc cgcggaacgt
      181 cttccaatcg aaatcgacct cgaggccctc ggcggcgaag aagccttctt gaagggcgac
      241 gaaatcataa agccccatgc cttttggagc gaccgtcacc ggtctcaagc tgttggccat
      301 ggcgattcct cctggaaccc gtcggtcgag tgcctgtcga gtttgcgctt acgccttctc
      361 taacgcattc cggccgggcg gtcactaacc cgcagcgtga cgcgagcggc cctacaggat
      421 cggcgccgag caaggctagc tgtagccatc ttgatctgca tgatacgtaa gaagaccatc
      481 atatccctgg cctaggatga cgacgagcat cgtggaatcg agccgcgcgc cgcttctcgt
      541 agacttcgcg ctgcaaggcg gtggcgcgca cggcgcgttc acctggggcg tgctcgaccg
      601 cctgctccag gaaacctggt tgcgcatcga tggtatctcc ggcacctcgg cgggggcgat
      661 gaatgcggcg gtgctggtcg acggctatgc cgacaatggc gtcgacgggg cgcgcgctgc
      721 gctggaaaat ttttggcgcc gcgtggccag ggctgccttg ctgagcccgc tacggcgtac
      781 tccgctcgac gtgctgctgg gtcgttggac gctcgaccat tcgcccgtct tcgtcgcgat
      841 ggacctgatg gcgcgcctgt tctctcccta cgatctcagc ccaggtggcg caaatccctt
      901 gcgcaatatc ctcgccgaga ccgtcgattt cgatcggttg gcgcaagcgt cgatcaagct
      961 cttcgtcacc gcgaccaacg tccgcacggg acgtggccgg gtcttccgaa acagcgaaat
     1021 cacgccggac gtgctgctcg cgtcggcgtg cctccccacg ctgttccagg cgatcgagat
     1081 cgatggcgaa agctactggg acggcggcta ctcgggcaat cccaccatca cgccgctggt
     1141 gcgcgaatgc cgctcccagg atacgattct cgttcagatc aatccggtcg aacgaccggg
     1201 gttaccgcgc tctgcccgcg acattctcaa ccggctcaac gaggtctcgt tcaacgccgt
     1261 cttgctgaaa gagctgcgca tgatcgcctt gctgcgccaa gttgcacagc cggataattc
     1321 cgagagcgcg aaatgggcgg acatgcggat ccaccgcatc tccagcgacg tgatggtgga
     1381 gctcggctat tcttcgaagc tcaacgccga gtgggaattc ctgtgcatgt tgcgcgacga
     1441 ggggcggcgc gccggtgatg cattcctgag cgcccatcac gacgatctgg gccggcgctc
     1501 caccttcgac ctcgatgagt tgctcaaggg cgtatgacac gatgggtctc attgggatcc
     1561 tactcggatt ggcgctgctc atatggcttg cgctgcgtgg ctggagcgtg ctccttctcg
     1621 ctccggccgc cgctctactt gccgccgcgt tcgcgctgga gccgctgctc gccaactgga
     1681 cccaaacctt catgggcagt gcggcggtct tcctggcgca gtttttcccg ttgtttctgc
     1741 tcggcgccct gttcggcaag ctcatggacg acagcggttg cgtatccgcc atcgcgggct
     1801 tcatgaccga acggctgggc gagcgccgcg ccgtgctggc ggtcgtactc gccggtgcgc
     1861 tcgtaaccta cggcggcgtc agcttgttcg ttgctttctt cgtgctcgtt cccatggcgc
     1921 aggcgttgtt ccgatcagcg tccattccgc gccgcttgat gccagcggcg atcacgctgg
     1981 gcacctccac ctttacgatg tcagccctgc cgggaacgcc agcgattcag aatgcgatcc
     2041 cgatgccatt tttcggcacc acgccattcg cggcgcctgg tctggggatc gtcgcatccg
     2101 ccatcatgct gggattcggc ctgtggtggt tgggtcgggc ggagcgtgcc gcccgccgcg
     2161 ctggagaggg ctacggcgct cctgacggca cggcgctcga tgccgcggca gacgatccgg
     2221 tcgtgcgcga gctcgccacc accgcacgcg agttcgatcc tgccgaaatc catcgcggcc
     2281 agcacggcga caccaaccgc ccatcgctcg ggcattcctg ccgctggccg tcgtggtgag
     2341 cgtcaacctg ctgatgtcgc tggtcctgct accgcgcctc gacttttcgt tcctggcgga
     2401 ggaacgttgg ggctccacct cgctctcggc ggtcgcaggc gtgtgggcgg tggtggttgc
     2461 gctggcatcg gcaatcgcca cgctcatcgc catcaattat cgctac
//
LOCUS       DATLJT010001153         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2840741,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001153 DATLJT010000000
VERSION     DATLJT010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2840741"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VM910_26615"
     CDS             <1..298
                     /locus_tag="VM910_26615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI66127.1"
                     /translation="SSLGAGDWRLFRTIILPSSLPFLLAGLRLAIGRGMIGVVVGEIY
                     GSAAGVGAMINQAGARFQTDKVFVGVLTIVAVGVILVEIVQRIERRVDVWRAPA"
     gene            314..1129
                     /locus_tag="VM910_26620"
     CDS             314..1129
                     /locus_tag="VM910_26620"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66128.1"
                     /translation="MHAKLEAQDIRLEYFQPRTNTWLLALDGVDLKIMDGEFVSIVGP
                     SGCGKTTFLSVVDGLIAASAGRILVDGQAVTKPGPDRAVVFQDASLLPWRTVLNNVRY
                     GLECLNVGAREAKERASHFIEMVGLSGFEHHYPYELSGGMQQRVNLARALVMDPKILL
                     MDEPFASLDAQTRELMQEELLQIWVQAKKTVLFVTHQIDEAIYLSDRVVVFSSRPGKV
                     KETVAVAIERPRRLTIKRGARFHAIEDRIWSLIEEDVKADRNRRNVDRSDRSF"
     gene            1176..>2099
                     /locus_tag="VM910_26625"
     CDS             1176..>2099
                     /locus_tag="VM910_26625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66129.1"
                     /translation="MRSGTLRITAASLGLAFALGAAAAPAQAADPIPFKIGISAPVVT
                     IFPVWMGEAGGFYEKEGLKVDVINMEGGTRGLQVLLSGEIQGMHVGLAPAVLANKQGA
                     DLRLVTSTCNTIPITMFTKAGVGMGDLKGKTFGISTFGSETDVAISILLKQLGLTRSD
                     VTVSQIGGSSQRFAALIAGRIDVAPLIEPGITLARERGFNPIIDLAAAKTPWIFDSVV
                     VSSSYLKGNRATLTRFVKAYVAGAYLALANETKAKEVIAQKFKTNDAKVIAATYADFK
                     RLMPRDAAPSLEGAKNVLAQLEAIGTDIGSRN"
BASE COUNT          384 a          651 c          688 g          376 t
ORIGIN      
        1 atcgagtctg ggcgccggcg actggcggct gtttcggacc atcatcctgc cgagctcgct
       61 ccccttcctg ctggcggggc tgcggcttgc gatcggccgc gggatgatcg gcgtggtggt
      121 gggcgaaatc tacggttcgg ccgcgggcgt tggcgccatg atcaaccagg cgggagcgcg
      181 ctttcagacc gacaaggtgt tcgtcggcgt gcttaccatc gtcgccgtcg gggtgatatt
      241 ggtcgagatc gtgcagcgga tcgagcgccg ggtcgatgtc tggcgtgcac cggcctgaca
      301 gcagcagcgc atcatgcacg cgaagctcga agcgcaggac attcggctcg aatattttca
      361 gccccgcacc aatacctggc tgttggcgct cgatggcgtc gacctgaaga tcatggacgg
      421 cgaattcgtg tcgatcgtgg ggccgtccgg ctgcggcaag acgacgttcc tctcggtggt
      481 cgacggcctg atcgccgcga gtgcgggccg catcctggtc gatggccagg cggtgacgaa
      541 gccggggccg gaccgcgcgg tcgtgttcca ggacgcttcg ctgctgccgt ggcggaccgt
      601 gctcaacaat gtccgctatg gactggaatg cctcaacgtc ggagcgcgcg aggcgaagga
      661 gcgtgcgtcc cacttcatcg agatggtcgg cctttccgga ttcgagcatc actatcccta
      721 cgagctttcc ggcggcatgc agcagcgcgt gaaccttgca cgcgcgctgg tcatggatcc
      781 gaagattttg ctgatggacg agccgttcgc atcgctcgac gcgcaaaccc gcgagctgat
      841 gcaggaggag ctgctgcaaa tatgggtgca ggcgaaaaag accgtgctgt tcgtgaccca
      901 tcaaatcgac gaagcgatct atctatcgga ccgcgttgtg gtcttttcca gccggccggg
      961 aaaggtcaaa gaaaccgtcg cggtggccat cgagcgcccg cgccggttga cgataaaacg
     1021 cggggcgcgt ttccatgcaa tcgaagaccg catctggagc ctgatcgaag aggacgtgaa
     1081 ggctgaccgc aaccgccgca acgttgaccg gagcgatcgc agtttctaga gcttggtgga
     1141 tccgctcgat cgataacgag gggtaggagg acgcaatgcg gtcaggcacg cttcgcatca
     1201 cggcagcttc gctcggcctc gcgttcgcgc tgggcgccgc ggcggcgccc gcccaagccg
     1261 ccgatccgat cccgttcaag atcggcatct cggccccggt cgtgaccatc tttccggtct
     1321 ggatggggga ggctggcggc ttttacgaga aggaagggct caaggtcgac gtcatcaata
     1381 tggaaggcgg cacgcgcggc ctgcaagtgc tgctttccgg cgaaatccag ggcatgcatg
     1441 tggggctcgc gccggccgtt ctcgccaaca agcagggagc ggacctgcgg ctggtgacgt
     1501 cgacctgcaa caccatcccg atcaccatgt tcacgaaagc cggggtcggc atgggcgacc
     1561 tcaagggcaa gaccttcggt atcagcacct tcggctccga gaccgacgtt gccatcagca
     1621 tcctgctcaa acagctcggc ctcacccgtt cggacgtcac cgtttcgcag atcggcggca
     1681 gctcgcagcg ctttgccgcg ctcatcgccg gtcgcatcga tgtcgctccg ctgatcgagc
     1741 cgggcatcac gctggcgagg gaacgaggct tcaatcccat catcgatctg gcggccgcga
     1801 agacgccgtg gattttcgat tccgtcgtcg tcagcagcag ctatctcaag ggcaatcgcg
     1861 ccacgctgac gcgcttcgtg aaggcttacg tcgccggcgc ctatctcgcg cttgccaacg
     1921 agaccaaggc gaaggaggtg atcgcgcaaa agtttaaaac caacgacgcg aaggtcatcg
     1981 cggccaccta cgccgacttc aagcgcctga tgccgcgcga cgcagcgccg tcgctcgaag
     2041 gcgccaagaa cgtgctcgcc cagctcgagg cgatcggaac cgacatcggc agccggaac
//
LOCUS       DATLJT010001154         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3299813,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001154 DATLJT010000000
VERSION     DATLJT010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3299813"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="hypD"
                     /locus_tag="VM910_26630"
     CDS             complement(<1..812)
                     /gene="hypD"
                     /locus_tag="VM910_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HVI66130.1"
                     /translation="MKYVDEFRDPAKAQTLINGIQALVAQIDICRSRPLQIMEVCGGH
                     THTIFRYGIQTMLPEQIELIHGPGCPVCVLPMGRVDDCVAIAEAPGVIFTTFGDAMRV
                     PGSRKSLMQAKAEGADVRMVYSPLDALSLARDNPGRQVVFFGLGFETTMPSTALTVLQ
                     AEREGIRNFSLFCNHITIVPTIKAILDSPELTLDGFLGPGHVSMVIGTRPYEFIARHY
                     HRPLVVTGFEPLDILQSIWMVLRQIKDGRCEIENQYTRVVPERGNAAGLEAVA"
     gene            complement(827..1507)
                     /locus_tag="VM910_26635"
     CDS             complement(827..1507)
                     /locus_tag="VM910_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVI66131.1"
                     /translation="MSTEDRLQSLYPFLHGRRQDAATLKAALIESVRQKALEHRTALD
                     EFFANNREAVIAAAETIAGVYRAGGRMFAMGNGGSSCDAAHFSVEFLHPITAGRVALP
                     AINLTADIAMTSAVGNDVGFTHIFTRQIIALGRANDGLIGFSTSGNSENLVHAFVKAR
                     EMGLKTIGLAGGTGGKMVAAELDHCLVVRTDSIHRVQECHVTIYHILWDLVHTLLADD
                     RGGLAATR"
     gene            complement(1532..1807)
                     /locus_tag="VM910_26640"
     CDS             complement(1532..1807)
                     /locus_tag="VM910_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HVI66132.1"
                     /translation="MCLGIPGRVIEITDAANNLALADFNGVKRVINLACVVDPTHTIE
                     DCVGDWVLVHVGFAMSRIDEREAAETLRVLDMLGEAQAEMMAIRNSA"
     gene            complement(1825..>2767)
                     /locus_tag="VM910_26645"
     CDS             complement(1825..>2767)
                     /locus_tag="VM910_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HVI66133.1"
                     /translation="KHGRAWVAESSLNLVEVQHHHAHVAACMAENGVPRDAPAVLGIV
                     LDGLGFGTDGEIWGGEFLLADYTEFHRLASLKPVAMPGGTQAVREPWRNLVAHLDAAF
                     GPQWAGCLDGTQLGRYLTTKQTQTLSAMIHNNVNAPRASSCGRLFDAVAAAMGVCPDR
                     VSYEGEAAARLEALAGRGPANVSQDGGGYPFAIEHQTETGMLRLEPRPMWAVLLSDLR
                     NQSEPSTIVRRFSHGLATALATVARQVVEDAGKTPNDVVAALSGGCFQNRLLTEELAR
                     LLRSAGFSILLHAEVPPNDGGLALGQAAVAAARLLKS"
BASE COUNT          528 a          876 c          830 g          533 t
ORIGIN      
        1 gcaaccgctt cgagccccgc cgcattgcct ctctccggca cgactcgcgt gtactggttt
       61 tcaatttcgc agcggccgtc tttgatctgc cgcagcacca tccagatcga ctgcagaatg
      121 tcgagcggct cgaaccccgt aaccaccagt ggacgatgat agtggcgcgc aatgaattcg
      181 tagggacgag tgccgataac catgctgacg tgaccaggcc caaggaaacc gtcgagcgtc
      241 agctcaggtg aatcgagaat ggctttgatc gtcggtacaa tggtgatgtg gttgcaaaag
      301 agggagaagt tgcggattcc ctctctctcg gcctgaagca cagtcagagc ggtcgacggc
      361 atcgttgttt cgaagccgag gccgaaaaac accacctgcc ggcccgggtt gtcgcgggca
      421 aggcttaggg cgtcgagagg ggaatatacc atacggacat cggcgccctc tgccttggct
      481 tgcatcaggc ttttgcgcga gcccggcaca cgcatggcgt cgccgaaggt cgtgaagatc
      541 acgcctggcg cctcggcgat ggcgacgcag tcgtcgacgc gacccattgg cagcacacat
      601 accggacagc cagggccgtg gatcaactcg atctgctccg gaagcatggt ttggatgcca
      661 tagcgaaaaa tcgtgtgggt gtgtccgccg caaacctcca tgatctggag cggccgactg
      721 cggcagatat cgatctgtgc gacaagggcc tgaatgccgt tgatcagcgt ctgcgccttt
      781 gccgggtccc ggaattcgtc gacgtatttc attcatccgc tccgcgtcag cgcgtggccg
      841 ccagcccgcc gcgatcgtcg gccaaaagcg tgtgcaccag atcccacagg atgtgataga
      901 tcgtgacgtg gcactcctgc acgcgatgga tgctatccgt gcgcactacg agacagtgat
      961 cgagctcggc tgccaccatc ttgccgcccg tcccccctgc aagtccgatg gtcttgaggc
     1021 ccatttccct tgccttgacg aaggcgtgga ccagattctc ggagttgcca cttgtggaaa
     1081 agccgatgag gccgtcgttc gcgcgcccaa gggctatgat ctggcgcgtg aaaatatgtg
     1141 tgaagccaac atcgttgccg accgctgatg tcatggcgat gtcagcggtg aggttgatcg
     1201 cgggaagggc gacacggccc gccgtgatcg ggtgcaggaa ctcgaccgaa aaatgcgctg
     1261 catcacagct ggaaccgcca ttgcccatgg cgaacattcg ccctcccgcc ctgtacactc
     1321 cggcgatcgt ttccgcggct gcgatcacgg cctccctgtt gttagcaaag aattcatcca
     1381 gcgctgtgcg gtgctctagg gccttttgcc gcacggactc tatcaacgcc gccttcagcg
     1441 tcgccgcatc ctggcgcctg ccatgaagga acggataaag gctctgcagc ctgtcctcgg
     1501 tagacataat gctctgtccc gatgttcgcg ttcaggcaga gtttcggatc gccatcatct
     1561 cagcctgggc ctcgccaagc atatcgagga cgcgcaacgt ctcggccgcc tcgcgctcat
     1621 cgatccgact catggcgaac ccgacgtgga ccaagaccca gtcgccaaca caatcctcga
     1681 tcgtgtgtgt cggatcgacg acgcaggcta gattgatcac gcgcttcacg ccgttgaaat
     1741 cggcgagcgc aagattgttc gctgcatcgg tgatctcaat caccctgccc ggtatgccaa
     1801 ggcacatgca agggttcgtc ctcgtcagct cttgagaagt cgcgccgcgg cgacggctgc
     1861 ctgcccgaga gccaaaccac cgtcgttcgg cggcacttcc gcgtgcaaca ggatactgaa
     1921 tcctgccgag cgcaacaagc gcgcgagttc ctccgttagc agccggttct gaaagcaccc
     1981 accagaaagc gcggcgacga cgtcattcgg agtctttccg gcgtcctcga cgacctgacg
     2041 ggcaactgtt gcaagggccg ttgcaagtcc gtgggaaaag cgccggacga tagtgctggg
     2101 ctcgctctgg ttccgcagat ccgaaagcag caccgcccac attgggcgcg gttcgagccg
     2161 aagcataccc gtctctgtct ggtgctcgat ggcgaacggg tagccgcccc cgtcttgcga
     2221 aacatttgcg ggtccgcgtc cggccagtgc ctcgagccgc gctgccgcct cgccctcgta
     2281 agacacgcgg tcggggcaga cccccattgc cgctgcaacc gcgtcgaaca gccgcccgca
     2341 cgagctggcg cgtggcgcat tcacgttgtt gtggatcatc gccgataacg tttgagtttg
     2401 tttggtggtc agatatctgc cgagttgtgt accgtcgagg cagcccgccc actgcggccc
     2461 gaatgctgcg tcaaggtgcg caacgaggtt gcgccagggc tcgcggacgg cttgagtccc
     2521 gccgggcatg gcgacgggct ttaggctagc cagccgatgg aactccgtgt agtcggccag
     2581 caggaactcg ccgccccaaa tctctccatc ggttccaaaa ccgagcccat caagcacgat
     2641 acccagcacc gcaggtgcat cgcgcggcac cccgttttcc gccatgcacg cggcgacatg
     2701 cgcatgatga tgttgcacct cgacgaggtt gagcgagctt tctgcgaccc acgcgcggcc
     2761 gtgcttg
//
LOCUS       DATLJT010001155         7959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3070250,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001155 DATLJT010000000
VERSION     DATLJT010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3070250"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VM910_26650"
     CDS             complement(<1..318)
                     /locus_tag="VM910_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66134.1"
                     /translation="MNRRDMLASTAVVLSSAGSISAVGVAGAQQSPEIDQVREAGTAL
                     YTSLSSLDVGRVEGFWAHESYVRYIGPTSKAVAVGWDEVKKAIGAGNAALSARRVVLT
                     QAHI"
     gene            complement(388..3591)
                     /locus_tag="VM910_26655"
     CDS             complement(388..3591)
                     /locus_tag="VM910_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVI66135.1"
                     /translation="MRFSHFFIERPVFATVLSLILMIAGVIAQQSLPISEYPEIVPPT
                     VNIKATYPGASAEVIAETVAAPIEQEVNGVDDMLYISSQSTGDGQLSINVVFKPGINI
                     DQAQVLVQNRLAVVQPRLPDEVTRFGITVRKSSPDLMMVVHMTSPDGSRDHQYIANYA
                     SLYIKDALLRIEGVGDILVFGARDYSMRVWLDPAKVAERGLTAGEVVTALRAANLQVA
                     AGSINQPPAKSPVAFQFAVQTLGRLSDPSQFENIMIRADADGGYVRLRDIARVELGSQ
                     DYTLNSYLNDNVATALVIRQRPGSNALATAATIKNTMQQLSRSFPSGVGYVIAYNPTE
                     FIQLSIDEVKETLLIAVFLVALVVILFLQTWRAAVIPVLAVPISLFGSFLVMRATGIG
                     LNTLSLFGLVLSIGIVVDDAIVVMENVERYLRQGCSPREAAHKTMDEVGSALVAIALV
                     LCAVFLPTAFISGLQGTFYKQFAITIAASTMISAFVSLTLSPALAAVLLKPHHDQPKA
                     AWLRRLGTPIRWLFTKFNSIIAALSKSYGWLTRRLIRVAFIALIIYGGLIALAFRQLS
                     TTPTGLIPQLDRGYLIAAFQLPPGASLDRTDEVVREAIKEIRQHPAVLDATAFVGFDG
                     ATFTNAPNTGAIFFTLKSFEERARRGFPAARIVPELRRQLSRFKDAFVIVLEPPSVPG
                     IGTGGGLKGYVQNRAGQSLPELERATWLVAGTASQTPGFTQAFTLFNTRTPQIYADVD
                     RTKSELLGVPINRVFEALSIYMGSIFVNDFNILGRTYRVTAQADNAYRLNLQDIANLK
                     TRNAVGEMVPIGATATFEDKTGPFRVPRYNLFPAAEVQLNLARGFSSGQGITAAERIA
                     KERLPSGFAFEWTEIALQEKLAGNTAVIAFGLAVVFVFLLLAALYESWILPLAVVLIV
                     PMCIFAAMIGVNMRGLDRNILVDIGLIVLIGLAAKNAILIVEFAKQAESAGMDRFEAA
                     VHAARTRMRPILMTSLAFIFGVLPLTIAVGAGAEMRQSLGIAVFAGMIGVTLFGLLFT
                     PAFYVAVRALPAHAMAWGEMRKRGSDLGVGDKHTRLG"
     gene            complement(3612..4784)
                     /locus_tag="VM910_26660"
     CDS             complement(3612..4784)
                     /locus_tag="VM910_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI66136.1"
                     /translation="MAFPPNIRSIAVLYRLTTIAASLVLVFGSSLPLNAQPKAPEVTV
                     ANPVLKRIVQWEEHTGQFEAVRRVEVRPRVSGELVKIHFTDGQTVKAGDILFTIDPRP
                     FEIAVEAARAELARARAQVAVTATDLERAEQLTPSKVLTQRDFDQRKANHDAAKAQLQ
                     SAEAMLRHAELNLDWTSVPAPIDGRISDRKVDAGNLVSGGQVGASLLTTIVSLHPIHF
                     VFNVSEPDYVRYSRLAANGQRPSSGVPGNAVQIRLADEKEWARNGKVNFIDNQLNGKS
                     GTIRARAVVENEDLFLTPGTFGRLRLFGGDGDALLIPDAAVVSDQSRKVVYTVGPDNK
                     IQVKPVTLGPIEESLRVVTTGLSKDDRVVISDLANPAVRSGAIVVPIPGSIVLATK"
     gene            complement(5031..5759)
                     /locus_tag="VM910_26665"
     CDS             complement(5031..5759)
                     /locus_tag="VM910_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVI66137.1"
                     /translation="MRDHPIVSQDEWLAARLRLLAKEKEFTRQRDRLSEERRALPWVK
                     VEKEYVFDGSNGRETLLQLFQGRSQLLIYHFMYGTGWKEGCKNCSFWADSFNGITAHL
                     KHRDVAMIVVSRAPYSKLRAFQERMGWSFKWVSSSTSDFGHDFNVSFTPEEMKGVVFY
                     NYERRKFPMTEAPGISVFYKDDIGTLFHTYSCYGRGLDAVNGAYQLLDLVPKGRDEAD
                     LPHPMSWVRHHDRYDASLEAPESA"
     gene            complement(5928..6098)
                     /locus_tag="VM910_26670"
     CDS             complement(5928..6098)
                     /locus_tag="VM910_26670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66138.1"
                     /translation="MAARAWFAIVFWMAVAVEVTIPTIKPWASAPECQSKSAEPIAAW
                     EIIYRPLHWNVY"
     gene            6277..7176
                     /locus_tag="VM910_26675"
     CDS             6277..7176
                     /locus_tag="VM910_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI66139.1"
                     /translation="MTAFTAGKPVDREAFDRLVGELRPRLHRYCARMTGSVLDGEDVL
                     HEAIVKAIEALSQTGSIAHPEAWLFRIAHNAALDFLRHRARQDAAHADEDPDMIVDPV
                     AVADDRQVAAASLRTFMRLPVAQRSSVILMDVLGYSLEEIGSVMDVSIATVKAALHRG
                     RARLRELAREPDDVPLPILAEPERLLLATYVERFNARDFNAIRDMLADEVRLDLVAKV
                     QRKGRTEVATYFDNYARLEDWHFVPGLMDGRPAVLVRNPSDPAGKVGYFILLQWEGDR
                     VANIRDFRFARYATEGAELFFME"
     gene            7220..>7959
                     /locus_tag="VM910_26680"
     CDS             7220..>7959
                     /locus_tag="VM910_26680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66140.1"
                     /translation="MQQLPQKKVVVLGGSRGVGRVIVQTFNAAGAQVMAVARNQDALT
                     VLNNEEPEVETLALDVTADCAPEAVFERINPDGLVICVGAKRTGSPFYELEWPEFAET
                     WDIDVKASFLFCRAAIRRPLQAGAVIILISSGAALGGSPISGGYAGAKRMQMFLANYA
                     QKESDRLGLDLRFLAVTPMRPMVDTEGGRAAVASYTKYLGISTEEFIRGMGSPQTAQD
                     VANAVLSFVTIPPDRASNVFVVSGTGIEA"
BASE COUNT         1679 a         2350 c         2350 g         1580 t
ORIGIN      
        1 aatgtgagcc tgcgtcaaaa ccactcttct cgccgataga gcagcattac ccgcgccaat
       61 ggcctttttg acctcatccc aaccgacggc aacggcttta ctcgtgggtc cgatataccg
      121 gacataggac tcgtgagccc aaaatccttc cacccgccca acatctagcg aggacaacga
      181 ggtatagagc gcggtgcccg cctctctcac ctggtcaatc tcgggcgact gctgggcacc
      241 ggcgacaccg acagctgaga tgcttcctgc gctactcagt accacggcgg tcgacgcaag
      301 catgtcgcgt cgattcatgg cctcctcctt gagtaaatca aaactctgtt gcagacagcg
      361 tgaaacggcc atggtctgcc gtacaaatca gcccagcctc gtatgtttat cgccgacgcc
      421 caagtcgcta cctcgtttgc gcatttctcc ccaagccatc gcatgtgcgg ggagagcccg
      481 gaccgccacg taaaaggccg gcgtaaacaa cagaccgaat agagtgacgc caatcatacc
      541 ggcgaagact gcaatgccga gcgactgccg catttctgcg ccggccccga cggctattgt
      601 gagcggcaga acgccaaaga tgaacgcgag ggacgtcatc aggataggcc gcatccgcgt
      661 ccgcgccgca tggactgccg cctcgaaccg atccatgccg gcgctttcgg cctgcttggc
      721 gaattcaacg atcaggatgg cgttctttgc agccaatccg atcagaacga tgagcccgat
      781 gtcgaccagg atatttcgat cgagacctcg catgttcacg ccgatcatcg cggcgaaaat
      841 gcacattgga acgatgagaa ccacagcgag cggcaaaatc cagctctcgt agagcgcggc
      901 gagcagcagg aatacaaaga cgaccgccag gccaaaggct atgactgcag tatttcccgc
      961 aagcttctct tgcagcgcga tctcagtcca ctcgaatgcg aagccgctcg gcaggcgttc
     1021 cttggcaatt cgctcagccg cggtgatacc ttggccggac gaaaagccac gcgccaaatt
     1081 cagctggact tcggcggctg gaaacagatt ataccgcggc actcgaaatg gtcctgtttt
     1141 atcttcgaat gtcgctgtcg cgccgatcgg caccatttct ccgactgcgt tgcgcgtctt
     1201 cagatttgca atatcttgca agttcagccg gtacgcattg tctgcctgcg cggtcacccg
     1261 ataggtgcgg ccaagaatgt tgaagtcatt gacaaagatg gagcccatgt agatcgaaag
     1321 cgcctcgaac acgcggttga tcggcactcc caacagctct gacttggtcc gatcgacatc
     1381 cgcgtagatt tgcggcgtgc gggtgttgaa cagcgtgaat gcctgggtga atccaggcgt
     1441 ctgagaagcg gtgccggcca cgagccacgt cgcgcgctcg agctccggca aagattggcc
     1501 ggcgcggttc tgaacatagc ctttcaatcc gcccccggtg ccaatgccgg ggaccgaagg
     1561 cggctcgaga acgatgacaa aagcatcctt gaagcgggac agctgccgac gcagctcagg
     1621 gacgatccgc gcagccggga aacctcggcg ggcgcgttct tcgaatgatt tcaaggtaaa
     1681 gaaaatcgca ccggtattcg gcgcgttggt gaacgtcgcg ccgtcgaaac cgacaaacgc
     1741 ggtcgcgtcc aacacggctg ggtgttggcg aatttccttg atagcctcac gaacaacctc
     1801 atctgttcga tcgagcgatg caccaggcgg aagttgaaat gccgcaatca gatagcctcg
     1861 atcaagctgc gggatgaggc cggtaggcgt cgtgctcagc tggcgaaacg cgagcgcgat
     1921 cagcccgcca taaataatca gggcgatgaa ggcaactcga ataaggcgtc tggtcagcca
     1981 gccatagctc ttagacagcg ctgcaatgat ggagttgaat ttcgtgaaca gccaacggat
     2041 cggcgtaccc aagcgccgca gccaggcggc ctttggctga tcatgatgcg gcttgagcag
     2101 gaccgcagcc agcgcgggag agagagtcaa cgagacgaaa gccgaaatca tcgtcgatgc
     2161 agcgatcgtg atggcaaatt gtttgtagaa cgttccctgc agcccgctga taaaggctgt
     2221 cgggaggaac accgcgcaca gaacgagcgc gatcgcgacc agcgcgcttc caacctcgtc
     2281 catcgtcttg tgggcggctt ccctcggaga acagccttgg cgcagatagc gctcgacgtt
     2341 ctccatcacc acgatcgcgt cgtcgacgac aatcccgata gaaagcacta ggccaaaaag
     2401 tgacagcgtg ttgagaccga tgccggtggc gcgcattacg agaaaggagc cgaataacga
     2461 gatgggaacg gcaagtaccg ggatgaccgc cgcgcgccaa gtctgcaaga agagaatcac
     2521 aaccagggcg acgaggaaaa cggcgattag gagcgtctcc ttcacctcgt cgatcgaaag
     2581 ctgaatgaat tcggtcggat tgtaggcgat gacatatccg acgccgctgg gaaagctacg
     2641 gctcagctgt tgcatagtgt tcttgatggt ggctgcggtt gccagcgcgt tcgagcctgg
     2701 acgttgtcgg atgacgaggg ccgtcgcaac attgtcattg agataagagt ttagagtgta
     2761 gtcctgcgaa ccgagctcca cccgagcaat gtcccgcaag cgaacgtaac cgccatcagc
     2821 atccgccctg atcatgatgt tttcgaattg actggggtct gagagccggc caagcgtttg
     2881 cacggcgaat tggaatgcga cgggcgactt ggcgggcggc tggttgatgg aaccggccgc
     2941 cacctgcaaa ttcgccgcac gcagggcggt aaccacttca cccgccgtca agccgcgttc
     3001 tgcaacctta gcggggtcga gccagacccg catcgaatag tcgcgggcgc cgaaaaccag
     3061 gatgtcgccg acgccctcga tgcgaaggag cgcgtccttg atgtagagcg atgcgtagtt
     3121 cgcgatatat tggtgatccc gcgagccatc cggtgaggtc atgtgcacga ccatcatgag
     3181 gtcaggcgac gatttgcgca cagtgatgcc aaatcgcgtg acctcgtccg gcaaccgcgg
     3241 ttgaacaacg gcaagccgat tctggactag aacctgggct tggtcgatat tgatgccggg
     3301 cttgaagacg acattgatgg agagctgccc atcgccggtc gattgggagc tgatatagag
     3361 catgtcgtcg acgccattta cttcctgctc gatcggggcg gccaccgttt cggcgatgac
     3421 ttcggccgac gcgcccggat acgtcgcctt gatgttgacg gtgggcggca cgatttccgg
     3481 atattccgag attggcagcg attgctgcgc gatcactcct gcaatcatta ggatcagcga
     3541 aagcaccgtc gcgaagaccg gacgctcgat gaagaaatgg gagaaccgca tcggcgatcg
     3601 tgtttctctg actatttggt cgccaggacg atgctgcccg gtatcgggac cacgatcgcc
     3661 cctgagcgaa cggccggatt tgctagatcc gaaatcacca cacggtcgtc cttggacaag
     3721 ccggtggtga ccacgcgaag actttcttcg attggtccca gcgttaccgg cttgacttga
     3781 atcttgttat caggtccgac cgtataaacg actttgcgac tctgatcgga caccacggcc
     3841 gcgtcgggga taagcagggc gtcgccgtcg ccaccgaaga ggcgcagacg cccgaatgtc
     3901 cccggcgtca agaataaatc ctcattctca accacggccc gggcgcgaat ggtgccggac
     3961 ttgccgttga gctggttgtc gatgaagttc acctttccat ttcgtgccca ttctttttca
     4021 tcggctagac ggatctgaac ggcgttgccc gggacgcccg atgacggtcg ctggccgttc
     4081 gccgccagac gcgagtagcg cacgtagtcc ggttcagaga cgttaaacac gaaatggatc
     4141 gggtggagac tgacgatagt ggtcagcagc gaagcgccga cttggccgcc ggagacgagg
     4201 ttgccggcgt cgacctttcg gtcggagatg cggccatcga tcggcgccgg caccgatgtc
     4261 caatcaagat tgagctccgc gtggcggagc atggcctcgg cggattgcaa ttgcgccttg
     4321 gccgcatcgt gattggcctt tcgctggtcg aagtcacgct gcgtgaggac ttttgacggc
     4381 gtcagctgct cggcgcgctc caggtcagtg gccgttacgg cgacttgtgc tctcgctcga
     4441 gccaactccg cgcgcgctgc ctcgaccgca atctcgaacg gtcttggatc gatggtaaag
     4501 agtatatccc ccgccttcac ggtctgaccg tccgtgaaat gaattttcac cagctccccg
     4561 gagacgcgcg gacgaacttc gacacgccgc accgcctcga attgaccggt atgctcttcc
     4621 cattgaacga ttcttttcag aaccggattt gcgaccgtga cctccggtgc cttcggctgc
     4681 gcgttcaacg gaagggaact gccgaacacc agtactagcg aagcagcaat ggtggtcagc
     4741 cgatacagga ctgctatcga gcggatattg ggtggaaacg ccatctcatt ctcctccttc
     4801 ggcgcggcct gatgggcgtt gaacgtcgaa gagagttgac agtcgctgag cgctggcttc
     4861 cgcggattcg agggcggtcg tgaccacgag cgcgtcgagc gcgaccatca acgacgccac
     4921 cgaggttaat acgagtaccc aggccctaag cgcttcttcg cttggctgtt tcattttttt
     4981 ctaccctgct tgctgtgagc attggctgcg gctttgccca ttacctcgtc tcacgcggac
     5041 tcgggtgcct cgagtgatgc gtcgtatctg tcgtgatggc gcacccaaga catcggatgc
     5101 ggcagatcag cttcgtcgcg acccttgggc acgagatcga ggagttggta agcgccgttc
     5161 actgcatcca ggccacggcc gtagcacgag tacgtgtgga agagtgtccc tatgtcgtcc
     5221 ttgtagaaca cgctgatccc cggtgcctcg gtcattggaa acttacggcg ttcgtaattg
     5281 tagaagacta ctcccttcat ctcctcgggc gtgaatgaaa cgttgaagtc gtgaccgaaa
     5341 tcgctggtgc tcgacgacac ccacttgaag ctccaaccca tccgctcctg aaaagccctc
     5401 agtttcgaat acggcgcgcg cgacaccaca atcatggcga cgtcgcgatg tttcagatgc
     5461 gccgtgatgc cgttgaagct gtctgcccag aacgagcagt tcttgcagcc ctccttccaa
     5521 cccgtcccgt acatgaaatg atagatcagc aactggctgc ggccctggaa caactgcaaa
     5581 agggtctcgc ggccgttcga cccatcgaag acgtactcct tttcgacctt cacccacggt
     5641 agcgcgcggc gctcctcgct cagccggtca cgctgccgcg taaactcctt ctccttggcc
     5701 agaagccgca gacgggcagc gagccactcg tcttgcgaaa cgatcggatg atccctcatg
     5761 gcaacctcac gcgtgagcat tgcggtctaa ctcgtcctca ccggggaacg gtctcaacct
     5821 tccaacatcg agtccggtcc tgaagccttc agtgaggagg ccttccggcg acaaagcgag
     5881 gcacatgcgc ccgccagcgg tcatggtggg gatcgcctac cgaagcttca gtacacgttc
     5941 cagtgtagag gccggtagat gatttcccac gccgcaatcg gctcagccga tttgctctga
     6001 cactctggcg cgctggccca cggtttgatc gtcgggatgg tcacttccac tgcaacggcc
     6061 atccagaaga cgattgcgaa ccacgctctg gctgccatat ccgcctcctc acgcccgcac
     6121 tcctcatggg ctcgcagcga ttggcgtcgc cacctgccca cgaccgagag acgaatggga
     6181 gtggcgaaag gattctcaga aaaaattttt tattccgccg aatccttagc ccgatcccgg
     6241 acgtcatata tcgaggtgag ccctcaggag ggtccaatga ccgccttcac ggccggcaag
     6301 cccgtggacc gcgaggcctt cgaccggctg gtcggagaac tacggccaag gctgcatcgt
     6361 tactgcgccc gcatgactgg ctcggtcctt gacggcgagg atgtgctgca cgaggcgatc
     6421 gtcaaggcga tagaggcatt atcccagacg ggatcgattg cgcatccaga ggcctggttg
     6481 tttcgcatcg ctcacaatgc ggcgctggat tttctgcggc accgcgcccg acaggacgcc
     6541 gcccacgctg acgaagatcc tgacatgatt gtcgaccccg tagccgtcgc ggacgaccgg
     6601 caggtcgcgg ccgcgagcct gcggaccttc atgcgacttc ctgtggcgca gcgcagcagc
     6661 gtcatcctca tggacgtgct cggctactcc cttgaggaga tcggcagcgt catggatgtg
     6721 agcattgcga ccgtaaaggc ggcactgcat cgcggccgtg cccgcctgcg cgagctagca
     6781 cgagagcccg acgacgttcc gctacctatt ctggcagaac ccgagcgctt gctactcgcc
     6841 acttatgtcg agcgcttcaa tgcgcgcgac ttcaatgcca tacgcgacat gctcgccgat
     6901 gaggtgcggc tcgacctcgt tgcgaaggtt caaaggaagg gtcggaccga ggtggctacg
     6961 tatttcgaca actatgcccg gctcgaggac tggcatttcg ttcccggatt gatggacggg
     7021 cgcccggcgg tgctcgtgcg caacccgtct gatcccgcgg ggaaagtggg gtatttcatt
     7081 ctgctgcagt gggaaggcga cagggtcgcg aacatccgag attttcgttt cgcgcgatac
     7141 gcgactgaag gcgccgagct gtttttcatg gagtgattgg atttgctgat ttgcaccatc
     7201 ttcaacaata ggagacatca tgcagcaatt gcctcagaag aaagtcgttg ttctcggagg
     7261 gagccgcggt gtcgggcgtg tcattgtcca aacgttcaat gcggccggcg cacaggtcat
     7321 ggcggtagcc cggaatcaag acgcgctgac cgtactgaac aacgaagagc cagaggtcga
     7381 aacactagcg ttggacgtaa cggcagactg cgctccagaa gccgtctttg aaaggataaa
     7441 cccagatggg ttggtaattt gcgtcggtgc aaaacgtact ggctcgccct tctacgagct
     7501 ggaatggccc gagttcgcgg aaacgtggga catcgacgtc aaggcctcgt ttctattctg
     7561 ccgagctgcg atacggcgtc ccctccaggc aggcgccgtg attattttga tttcgagcgg
     7621 cgcagctctc ggggggtcgc ccatctcagg cggatatgca ggtgccaagc gtatgcagat
     7681 gtttctggct aactatgcgc aaaaggaatc cgatcggctt ggcctggatc tgcgctttct
     7741 ggcagtgacg ccgatgcgcc ccatggtgga cacggaaggc ggccgtgccg cagtagcgag
     7801 ctacaccaag tatctcggca tctccacaga agagttcatc agaggaatgg gctcaccaca
     7861 gaccgctcaa gacgtcgcca atgccgttct gagcttcgtc acgatcccgc cggaccgagc
     7921 gagcaatgtg ttcgttgtgt ccggcacggg catcgaggc
//
LOCUS       DATLJT010001156         8134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2150704,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001156 DATLJT010000000
VERSION     DATLJT010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150704"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="gmk"
                     /locus_tag="VM910_26685"
     CDS             <1..613
                     /gene="gmk"
                     /locus_tag="VM910_26685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVI66141.1"
                     /translation="GLMLVLSSPSGAGKTTLSRKLLDADPGVELSVSVTTRKQRPGEV
                     DGRDYHFIDAARFEAMIRGGELLEWAHVFGHRYGTPRAPVEAALARGHDVLFDIDWQG
                     TQALSEKAGRDLVSIFVLPPSMADLEQRLRRRAQDSDEVIHARMATAADEMSHWAEYD
                     YVVINTDVDRAFAQVQSILAAERLKRERRTGLSEFIRRLQAQL"
     gene            complement(676..1518)
                     /gene="rsmA"
                     /locus_tag="VM910_26690"
     CDS             complement(676..1518)
                     /gene="rsmA"
                     /locus_tag="VM910_26690"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HVI66142.1"
                     /translation="MSAVDDLPPLREVIRRHGLRAKKSLGQNFLLDLNLTARIARAAG
                     PLEGVDVVEIGPGPGGLTRALLAAGARQVIAVERDDRAIAALDEIAVRYPGRLRIVAG
                     DALNFDPRPLLGSSRARVIANLPYNIATALLVCWLTLEPWPPWYDRLVLMFQREVAER
                     VVAAPGCKSYGRLSVLVGWRCQARIRFDVAGSAFVPPPKVTSSVVELVPRENPLVCER
                     GALERVTEAAFGQRRKMLRQSLKSLGTDPSALLDAARIEPTARAEEVPIEGFVALARA
                     FAAR"
     gene            complement(1515..2543)
                     /gene="pdxA"
                     /locus_tag="VM910_26695"
     CDS             complement(1515..2543)
                     /gene="pdxA"
                     /locus_tag="VM910_26695"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVI66143.1"
                     /translation="MARPLALTLGEPAGIGPDITLAAWRRRAELNLTPFYFLADPTFV
                     VRRAKRIAPDLAIAVVEPAAAWTAFKTALPVVDIGVAVSAEPGQPDQSSAPAALAAIR
                     RAVADVRAGAAAAIVTNPVAKNVLYRSGFPEPGHTEYLGKLVEESTGAAARPVMMLWS
                     PELAVVPVTIHLPLKDVVAKLTADLVVETGRIVARDLHERFGIARPRLAIAGLNPHAG
                     EDGALGDEDLTLVGPAVERLKAEGIDARGPLPADSLFHQAARASYDVALAMYHDQALI
                     PIKTLAFDHAVNVTLGLPFVRTSPDHGTAFDIAGTGRADPTSLIAALQLAARLSSRSH
                     SHPVALRA"
     gene            complement(2555..3508)
                     /locus_tag="VM910_26700"
     CDS             complement(2555..3508)
                     /locus_tag="VM910_26700"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVI66144.1"
                     /translation="MDEEADEAMMLESFTTCRKLVTAVILVFGVSMLATAANAQQVVV
                     IVNGEPITALDIEQRSKLILLSTHKAPARQEVLDELINEKLKVREAKKFGLEIPSSEV
                     DTAYASMAGRMRITPEQLTEQLAKSGIHASTLKARIKADITWSQLVRGRYQSSLQIGD
                     KDILTAMESKSNDTVSYDYTLRPILFFVPPGSPDTVVEGRKREAEALRGRFQGCDDGI
                     TFARALKDVTVREQIIRSSADVPAELRKVLDGIEVGRLTPPEVTKFGVEMFAICAKKE
                     SAADDSLGRRRARETIMVERYEQRSKQYLQELRRGAMFEYK"
     gene            complement(3586..6054)
                     /locus_tag="VM910_26705"
     CDS             complement(3586..6054)
                     /locus_tag="VM910_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HVI66145.1"
                     /translation="MARAATHSRLSRRRSWWAALVIFAAAVTVVAGSAGVSPALAQLL
                     VFPPRPKPPVRPERSQEQMLVRAEEINYDYTNERVSAVGNVQLYYGNSTLEADRVIYD
                     QKTKRLHAEGNVQLTEGDGTVTHGEIMDLSDDYRDGFVDSLRLDAPEQTRFAAPRAER
                     TGNYTVFYNGVYTACEPCVQDPTKPPKWQVKAARIIHDQGEKMLYFEDAQLEFVGVPL
                     AYLPYMSAPDPTVKRKTGFLTPNYSSSSVYGFGVTIPYYWALAPDYDFTFAPMITSKQ
                     GPLLQGEWRQRLLDGAYSIRATGIFQLDRDLLVNTPGDRNFRGSLDTAGQFNISDKWV
                     WGWNGTVLSDKNYLYDYGLIRNLQIPTNLLNVSVDYALSQVYLAGRGDRSYFDARALY
                     FYGLSPVDNQSQLPVVHPVVDHDYVFNSPIFGGELSFHNNLTSISRETANFDAISQAA
                     VNGALCAQTADPALINSNNCLLRGVPGNYTRASTEWNWKHTIIDSWGQMFIPFFSVRA
                     DFADMNISNQPGVSNFINAGQSDVGRVMPVAGLEYRYPLINVQSWGTQTVEPIAQLIF
                     RPNETMVGSFPNEDAQSLIFDDSNLFKVNKFSGWDRVEGGGRANAGVQYTAQFNQGGN
                     VNVLFGQSYQLYGLNSFTLGGTTNTGLDSGLDSRQSDYVARASYQPNSLLTFTSRFRF
                     AENDFTLQRTELETTANFGRWTTMVMYGNYAAQPLIGFLERREGILGSARFKINPNWV
                     LLGAAGYDIHHKSVTQTQVGVGYIDDCLILALNYITSYAYSGSVTVNHSYMMQLTLRT
                     LGGTTSTQTAGVPVVNPGVFGGTH"
     gene            complement(6054..7142)
                     /gene="lptG"
                     /locus_tag="VM910_26710"
     CDS             complement(6054..7142)
                     /gene="lptG"
                     /locus_tag="VM910_26710"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HVI66146.1"
                     /translation="MITGTLSRYFGWRFLSAVLAVFAGTLVLTAMIDFLELLRRSADI
                     KDVSALLIAQITLFRVPFITERVMPFAVLVGAMFCYLNLSRRLELVVARAAGISAWQF
                     IAPAIVIAALIGIALTTVYNPISANLREHSTRLEADLSGRDSSFRSTGRGFWVRQRSE
                     DGQSVINAKSSRQQGIELGGVSVFRFDAAGHFRDRIEAKSATLEPGYWRLEDARFYAP
                     GAAPSDRESFLLQTSLTPVQVGESFATPETVPFWELSSYIDHAESTGLAAAGYRLQYY
                     QLLAQPFYLAAMVLLAASVSLRFFRFGGVQKIVLGGIGAGFLLYILSKITGDLSKAGL
                     MGPLAAAGLPPAVGSVTGLIALLYQEDG"
     gene            complement(7139..>8134)
                     /locus_tag="VM910_26715"
     CDS             complement(7139..>8134)
                     /locus_tag="VM910_26715"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HVI66147.1"
                     /translation="VLIIAPIALMIAVAHVLNKLGNDSELIVMNAAGMPPWFLFRPFL
                     AAGIVVSLLVAAISTYVSPWGLRELRRWATEVRADLVSNIVQPGRFIKLEERLTLHIR
                     ERRPNGQLLGIFIDDQRDPKERASILAEQGDIVKNERGLFLVLENGSVQRHETGQRDP
                     ALVLFNSYGFDLSWLSNTNQSLKYSVRERYLWELYNPALTTMTFSDEPDQIRAEFHDR
                     ITAPLYPLAFVVMTYAYLGAPRTTRQNRTMSLMGAISVVAALRGLGFVGMIAGVHTPL
                     ALALPYLALTAAFVLGYIAISRGVIIEPPAFVANAITAIMERIAQRTNALMGQAP"
BASE COUNT         1505 a         2475 c         2687 g         1467 t
ORIGIN      
        1 cgggctcatg ctggtgctgt cctcgccctc gggcgcgggc aagaccacgc tgtcgcgcaa
       61 gctgctcgac gccgatccag gcgtcgagct ttcggtctcg gtgacgacgc gcaagcaaag
      121 gccgggcgag gtcgacggcc gtgattatca tttcatcgac gccgcccggt tcgaggccat
      181 gatcaggggt ggcgagctgc tggagtgggc gcacgtgttc ggccatcgct acggcacgcc
      241 gcgcgcgccg gtggaggctg cgctcgcgcg cgggcacgac gttttgttcg acatcgactg
      301 gcaggggacg caggcgctta gcgaaaaggc gggtcgcgac ctcgttagta tcttcgtgct
      361 gccgccgtca atggcggacc tcgagcagcg gctgcggcgg agagcgcagg attccgatga
      421 ggtgatccac gctcgcatgg ccacagcggc cgacgaaatg agtcactggg cggagtacga
      481 ctacgtcgtg attaacaccg acgtcgatcg cgccttcgcg caggtgcaat cgatcctggc
      541 ggccgagcgc ctcaagcgcg aacggcggac cgggctctcc gagttcatcc gccggctgca
      601 ggcgcagctg tgacgccgaa tcatcggggg ccgcgggcga agagagactc gcgcgcgtcg
      661 cctcatcggc agcaatcagc gcgcggcaaa ggcccgcgcg agcgcgacga agccctcgat
      721 cgggacctcc tcggcccggg cggtcggctc gatcctcgct gcgtcgagca acgccgacgg
      781 atcggtgccg agcgatttca ggctttggcg cagcatcttg cggcgctggc cgaacgcggc
      841 ttcagtcacc cgctcgagcg cgccgcgctc gcagacgagc ggattttcgc gcggcaccag
      901 ctccacaacg gatgacgtca cttttggtgg cggcacgaag gcgctgccgg cgacgtcgaa
      961 gcggatgcgc gcctggcagc gccagccaac gaggacggac aggcgtccgt agctcttgca
     1021 gccgggggcg gcgacaacgc gttcggcgac ctcgcgctgg aacatcagca cgagccggtc
     1081 gtaccagggc ggccacggct cgagcgtgag ccagcagacc aacaacgcgg tggcaatgtt
     1141 gtaaggcagg ttcgcaatga cgcgcgcgcg ggagctgccg aggagcggac gcggatcgaa
     1201 gttgagggca tcgccggcaa cgatcctgag gcgtcccgga taccgcacag cgatctcgtc
     1261 gagcgccgcg atcgcgcggt cgtcgcgctc gaccgcgatc acctggcgcg cgcctgcagc
     1321 gagaagcgca cgggtgagcc cgcccggccc gggaccgatc tcgacgacgt ccacgccctc
     1381 gagcggacct gccgcgcgcg cgatgcgagc ggtgaggttg agatcgagga gaaaattctg
     1441 gccgagcgac ttcttggctc tgagaccgtg tcggcggatg acctcgcgga gcggcggaag
     1501 atcatcgacg gcgctcatgc gcgcagcgcg actggatgcg agtgcgaccg ggacgaaagc
     1561 ctcgcggcaa gctgaagagc ggcgatcagg ctcgtgggat cggcgcgacc ggtgccggcg
     1621 atgtcgaagg cggtgccgtg atcgggtgag gtgcgcacga acggcaggcc cagagtcacg
     1681 ttgaccgcgt gatcgaaagc cagcgtcttg atcgggatca gcgcctggtc gtgatacatc
     1741 gcaagcgcca cgtcgtagct cgcgcgcgcg gcctgatgga acagcgagtc cgcgggcaac
     1801 gggccgcgcg catcgatacc ctcggccttg agacgttcga cggcgggacc taccagcgtc
     1861 aggtcctcgt cgccgagcgc gccatcttcg cccgcatgcg gattaaggcc ggcaatggca
     1921 aggcgcggcc gcgcgatacc gaagcgctcg tgcaggtcgc gggcgacgat gcggccggtc
     1981 tcgaccacga gatctgcggt gagcttggcg acgacatcct tcagcggaag gtggatggtg
     2041 accggcacca ccgcgagttc gggcgaccac agcatcatca ccggccgggc agccgcgccg
     2101 gtcgattcct cgaccagctt accgagatat tcggtgtgtc cgggctcggg aaagcccgag
     2161 cggtagagga cgttcttggc gaccggattg gtcacgatcg cggccgccgc tccggcgcgg
     2221 acgtccgcca ccgcgcggcg aatggctgcc agcgccgccg gcgcgctcga ttgatccggc
     2281 tggcccggtt ctgcgctcac cgccacgccg atgtcgacga cgggtagcgc ggttttgaag
     2341 gctgtccagg ccgccgccgg ctcgaccact gcgatggcga gatcgggcgc gatccgcttc
     2401 gcgcgtcgca cgacaaatgt cggatcagca aggaaataga agggcgtgag gttgagctcg
     2461 gcgcgccggc gccacgccgc caaggtaatg tcgggtccga tcccggccgg ctcgcccagc
     2521 gtcagcgcca aaggccgtgc cattacgctt agccttactt gtattcgaac atcgcgccgc
     2581 gccgtagctc ttgcaaatac tgcttggatc gctgctcgta gcgctcgacc atgatggttt
     2641 cacgggcccg gcgcctaccc aatgagtcgt cggccgccga ctctttcttg gcgcagatcg
     2701 cgaacatctc gactccgaac ttggtgactt cgggtggcgt caatcggccg acctcgatgc
     2761 cgtcgagcac tttgcgcagt tccgccggaa cgtcagccga gctgcgaatg atctgctcgc
     2821 gcacagtgac atccttgagg gcgcgagcaa aggtaattcc atcatcgcaa ccctgaaaac
     2881 ggccgcgcag agcttccgcc tcgcgcttgc gcccctcaac gactgtatcc ggcgagccgg
     2941 gcgggacgaa gaaaaggatc ggtcgtaagg tgtagtcata gctgacggta tcgttggatt
     3001 tcgactccat cgccgtgagg atatccttgt cgccgatctg caggctggac tggtatcgcc
     3061 cgcgcacgag ttgcgaccaa gtgatgtcag ccttgatgcg agctttaagc gtggacgcgt
     3121 ggatgcctga cttggcgagc tgctcggtca attgttcagg cgttattcgc atgcggcccg
     3181 ccatcgaggc ataggcggtg tcgacttcgg agctgggtat ttccagtccg aatttcttgg
     3241 cctcgcgtac cttgagcttc tcgttgatca gctcatcgag gacctcttgg cgcgcaggag
     3301 ccttgtgcgt cgacaataga atgagcttgc tgcgctgttc gatgtcgagc gccgtgatcg
     3361 gctcgccgtt gacgatgacc accacctgct gggcgttggc ggcagtcgcc agcatcgaca
     3421 cgccgaatac gaggattacg gcggtcacca gtttgcggca ggtcgtgaaa ctctcgagca
     3481 tcatggcttc gtccgcttcc tcgtccatgg gagccggcac gttgcttgtc gctcgcgtgc
     3541 cgtgatcctt ctccagttca gccgttctcc ttaccgcgcg cgtggctaat gcgtgccgcc
     3601 gaataccccg ggattgacga cggggacgcc tgcggtctgg gtgctggtag tgccgccaag
     3661 cgtgcgcaag gtgagctgca tcatgtagct gtggttgacc gtcacgctgc cgctatacgc
     3721 gtagcttgta atgtaattca gggccaagat gaggcagtcg tcgatgtatc cgacgccgac
     3781 ctgggtctga gtgaccgact tgtggtgaat gtcatatccc gctgcgccca gcaacaccca
     3841 gttcgggttg atcttgaacc gtgcgctgcc gaggatgccc tcgcggcgct cgaggaaccc
     3901 gatcaacggc tgggcggcat agttgccgta catcaccatg gtcgtccagc ggccgaagtt
     3961 ggcagtggtt tccaactcgg tgcgctgaag cgtgaagtcg ttctcggcaa aacggaagcg
     4021 cgaggtgaag gtcagcaacg agttcggctg gtacgacgcg cgcgccacgt aatctgactg
     4081 cctgctatcg agtccgctat cgaggccggt attggtcgtg ccgccgagcg taaaagagtt
     4141 cagtccgtag agctggtagg actggccgaa caggacgttg acgtttccac cctgattgaa
     4201 ctgggcggta tattggacgc ccgcattcgc ccggccgccg ccttccacgc ggtcccaacc
     4261 ggaaaacttg ttcaccttga acagatttga atcgtcgaag atcaggcttt gcgcatcctc
     4321 gttggggaac gagccgacca tggtctcgtt cggccggaaa atgagctggg cgatcggctc
     4381 gaccgtctgc gtgccccagg attgcacgtt gatgagcggg tagcggtatt cgaggccggc
     4441 caccggcatg acgcgcccga catcgctctg accggcgttg atgaaattgg agacgcctgg
     4501 ctgattggag atgttcatgt cggcaaagtc cgcgcgcacg ctgaagaagg ggatgaacat
     4561 ttggccccac gagtcgatga tagtgtgctt ccaattccac tcggtggacg ctcgagtgta
     4621 attgccgggg acgccgcgca gcaggcaatt gttcgagttg atcagcgctg gatcggcggt
     4681 ctgggcgcag agcgcgccat tgaccgcggc ctgcgaaatc gcgtcgaagt tggcggtctc
     4741 gcgcgagatg ctggtcaaat tattgtggaa gctgagttcg ccgccgaata tcgggctgtt
     4801 gaagacataa tcatgatcga ccaccgggtg cacgaccggg agttggctct ggttgtccac
     4861 aggtgagagg ccatagaagt agagcgcccg cgcgtcaaag tagctgcggt cgccgcgacc
     4921 cgcgaggtag acttgggaca acgcgtaatc cacgctgaca ttgagcaaat tggtcggaat
     4981 ttgcaggttt cggatcaggc cgtaatcgta gaggtagttc ttgtccgaaa gcaccgtccc
     5041 gttccagccc cacacccatt tgtcggagat gttgaactgc cccgctgtgt ccaggctgcc
     5101 gcggaagttg cgatcgcccg gcgtgttgac gagcagatct ctgtcgagct ggaaaatacc
     5161 cgtggcgcga atgctgtaag caccgtcgag cagccgttgg cgccactcgc cttgcagcaa
     5221 ggggccctgc ttgctcgtga tcatgggcgc gaaagtaaag tcgtaatcgg gcgcgagcgc
     5281 ccaatagtag gggatcgtga ccccgaaccc gtagactgag ctgctgctgt agtttggcgt
     5341 gaggaagccg gtcttgcgct tcaccgtcgg atcgggcgcc gacatgtacg gcaggtaagc
     5401 caatgggacg ccgacgaatt cgagctgcgc gtcctcgaag tacagcatct tctcgccttg
     5461 atcgtggatg atccgcgctg ccttcacctg ccatttcggc ggcttcgtgg gatcctgcac
     5521 gcacggctcg caggcggtgt agacgccgtt atagaagacg gtgtaattgc ccgtgcgctc
     5581 cgcgcgcggc gcggcgaagc gagtctgctc gggcgcatcg agtcttagcg aatccacgaa
     5641 gccgtcgcgg tagtcgtcgc tgaggtccat gatttcgccg tgagtgaccg tgccatcacc
     5701 ttcggtcagc tgcacatttc cttcggcatg cagtcgcttg gtcttctggt cgtagatcac
     5761 ccggtcggct tcgagcgtgg agttgccata ataaagttgc acgttgccga ccgccgagac
     5821 gcgctcgttg gtgtagtcgt agttgatctc ctcggcgcga acgagcatct gctcctgcga
     5881 acgttcgggc ctgacgggcg gcttcggccg cggcgggaat accagcaact gggccagcgc
     5941 cggcgacacg ccggccgagc cggcgacgac cgtaacggcg gccgcaaaaa tgaccaacgc
     6001 cgcccaccac gagcggcggc gcgacaggcg cgaatgggta gcggcccgcg ccatcacccg
     6061 tcctcctggt agaggagcgc aatcaggccc gtcacgctgc cgaccgcggg aggcaatcct
     6121 gccgccgcca gcgggcccat cagtcccgct ttgctcaagt ctccggtgat tttcgacaag
     6181 atgtagagca gaaagcccgc accgatgccg cccaggacga tcttctgcac gccgccaaat
     6241 cggaagaaac gcaaactgac cgacgcggcc aaaagcacca tggccgcgag atagaagggc
     6301 tgtgccaaca gttgataata ttgcagtcga taaccggcgg cggccaagcc cgtgctttcg
     6361 gcgtggtcga tatacgacga cagttcccag aacgggacgg tctcgggcgt ggcgaagctt
     6421 tctccaacct gtaccggcgt taggctggtc tgcagcaaga aactctcgcg atccgacggc
     6481 gctgctccag gggcgtaaaa tctggcgtct tcgagccgcc agtatcctgg ttcgagggtt
     6541 gcgcttttcg cctcgatgcg gtcacggaag tgaccggcgg catcgaagcg gaaaacgctc
     6601 acgccgccca attcgatgcc ctgttggcgg ctcgatttcg cgttgatgac cgactggccg
     6661 tcctcgctgc gttggcgtac ccagaatccg cgtcccgtgc tgcggaagct gctgtcgcgg
     6721 ccggacaggt cggcttcaag gcgcgtcgaa tgctcgcgca agttcgccga gatcggatta
     6781 tagaccgtcg tcaacgcgat cccgatcaag gcggcgatga cgatggcggg tgcgatgaat
     6841 tgccaagctg aaattcccgc cgcccgcgcg accacgagct ccaagcgacg tgacaaattc
     6901 aaatagcaga acatcgcgcc gaccagtacc gcgaaaggca tcacgcgctc ggtgatgaag
     6961 ggtacgcgaa agagcgtgat ctgggcgatg agcaaggcgg agacgtcctt gatatccgcc
     7021 gagcgccgca acaattcgag aaaatcgatc attgcggtca gcaccaacgt ccccgcgaac
     7081 acggccagca ccgcggagag gaagcgccac ccgaagtatc gtgagagcgt cccggtgatc
     7141 atggcgcttg tcccatcagg gcgttggtgc gctgagcaat gcgctccatg atcgcggtga
     7201 ttgcattggc gacgaaggcc ggcggctcga tgatgacccc gcgtgaaatg gcgatatagc
     7261 ccagcacgaa tgctgcggtc agagcgagat agggaagcgc cagggcaagc ggcgtgtgca
     7321 ctcctgcgat catgccgacg aagcccaatc ctcgcagcgc cgccaccacg ctgatcgcac
     7381 ccatgagcga catggtgcga ttctgccggg tcgtgcgcgg cgcgccgaga taggcgtagg
     7441 tcatcaccac gaaggcgagc gggtagaggg gcgccgtgat ccggtcgtgg aattcggcac
     7501 ggatttgatc cggctcgtcg gaaaacgtca tcgtggtcag cgcaggattg taaagctccc
     7561 acagataccg ttcccgcacc gaatatttca ggctctggtt ggtgttcgag agccaggaca
     7621 ggtcgaaccc gtaactgttg aacagcacga gcgcgggatc gcgctggccg gtctcatgcc
     7681 gctgcacgct gccgttctcg agcacgagaa acaggccgcg ctcgtttttt acgatatcgc
     7741 cttgctcggc caggatgctt gctcgttcct tcgggtcgcg ctgatcgtcg atgaagatgc
     7801 cgagcaattg tccgttgggc cgccgctcgc gaatgtggag cgtaagcctc tcttccaatt
     7861 tgatgaaacg gccgggctgc acgatattgg agaccaggtc ggcgcggact tcggtcgccc
     7921 agcgccgcag ctcgcgcagt ccccacggcg agacataggt gctgatcgcg gcaacgagca
     7981 gcgaaacgac gatcccggcc gccaggaagg gacggaatag aaaccatggc ggcattccgg
     8041 ccgcattcat cacgatgagc tcggaatcgt tgcccagctt gttgagcacg tgcgccaccg
     8101 cgatcatcag ggcgatcggc gcgatgatca gcac
//
LOCUS       DATLJT010001157         1966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1692293,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001157 DATLJT010000000
VERSION     DATLJT010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692293"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VM910_26720"
     CDS             <1..1258
                     /locus_tag="VM910_26720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66148.1"
                     /translation="TSGREQDVATAKTDALTRWREQRNLPWLVAALMHLSPGDEDVAA
                     AIAASRAIEASSPAYYTLAWNRLRLLIGENKQEEARAELDQILDGSPLPEGVENLMRY
                     HRMKLARDLDEFVRFALRRGEFVMYLPDPRTKLDATAPPLKSSTSFPYDFGAMLKWRT
                     ELFQPNPRYFDMDATAAMSFVMPLPLMARVALSDRLPPNMQRDVALAVWTRAVLLDDA
                     EIASSVMPTVARHFPQYGAGWKAYQGAATPQQKKIEAALLLLRLPGASPWLASGLGYP
                     YMRDVIGLVATRWWERGDADNQAVPDVPDDVTLCDDCALPLQFAAPAFLTPQDRERAR
                     GEVARLRQLPGAPAYLGAIIIAWANAQPRDPRVPEALHLVVRATRYGQMNTETSKAAY
                     LLLHNRYRRNPWTSKTPLWFTAPRDE"
     gene            complement(1528..1752)
                     /locus_tag="VM910_26725"
     CDS             complement(1528..1752)
                     /locus_tag="VM910_26725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66149.1"
                     /translation="MTTSDDVQKRNAFAACAAFRIEGLEASLDQAREIAVRDELGAVG
                     HYIAEAQGYLAQIRLLHQEALDEFSRAQGE"
BASE COUNT          330 a          657 c          615 g          364 t
ORIGIN      
        1 cacctccggt cgcgagcagg acgtagccac cgccaaaacg gatgcgctca cccgatggcg
       61 cgagcagcgc aatctgccgt ggctcgtcgc cgcgctgatg catctctccc ccggcgacga
      121 ggatgtcgcg gctgcgatcg ctgcctcgcg cgcgatcgag gcgagctcgc ccgcctatta
      181 cacgctggcc tggaatcgcc tgcgcctgct catcggcgag aacaagcagg aggaagcgcg
      241 cgccgagctc gatcaaatcc tcgacggcag cccgctgccg gaaggggttg agaacctgat
      301 gcgctatcac cgcatgaagc tggcgcgcga cctcgacgag tttgtccgct ttgcgctgcg
      361 ccgcggcgaa ttcgtgatgt accttcccga cccgcgcacc aagctcgacg cgacggcgcc
      421 gcctttgaag tcgtcgacga gctttccgta cgacttcggc gcaatgctca agtggcggac
      481 ggagttgttt cagccgaacc cacgatattt cgacatggat gcgacagcgg ccatgtcgtt
      541 cgtcatgccg ctgccgctga tggcgcgggt cgcgctctcc gaccggctgc cgcccaacat
      601 gcagcgcgac gtggcgcttg cggtctggac gcgcgcggtg ctgctcgacg acgccgagat
      661 tgccagttcc gtgatgccca ccgttgcgcg tcatttcccc caatacggcg cgggctggaa
      721 agcctatcag ggcgccgcca cgccgcagca aaagaaaata gaagcggcgc tgctgctcct
      781 gcggctgccc ggcgcaagtc cctggctcgc ttccgggctc ggctaccctt acatgcgcga
      841 cgtgatcggg ctcgtcgcaa cgcgctggtg ggagcgtggg gacgccgata atcaagccgt
      901 gcccgacgtc ccggacgacg tcacgctttg cgacgattgc gcccttcccc tgcaatttgc
      961 tgcgccggcc ttcctgacgc cgcaggatcg cgagcgtgcg cgcggcgagg tcgcacgcct
     1021 gcgccagctt cccggagccc cggcctatct cggcgccatt attatcgcct gggccaatgc
     1081 gcagccgcgc gatccgcggg tgcccgaggc gcttcacctc gtggtgcggg caacgcgata
     1141 cggacaaatg aataccgaga cgtcgaaggc agcctatctg ctgctgcaca atcgttatcg
     1201 ccgcaatccc tggacgtcga aaacacccct ttggtttaca gcgccccgcg atgaatgagg
     1261 gtgagagggc ccccctgtca tccaaagccg tcgctgccag ccgggcaccg actgcaagct
     1321 ggaacgtggc catgcgctta ggcccccatc cggggcagac gcctcaagag atacgcgatg
     1381 cggacgtttc aaccgtgtgt ctcaatggcg gacaacgcgg cttgcagagc gagccttaag
     1441 cccaacgatg cgcggcaggc gcttgctgcc gccgctcgac cgttgacggc gtgagggttt
     1501 caagggcggc acggcggagc gagcgcctca ttcgccctgc gcgcggctga actcgtccag
     1561 cgcctcttga tgcaagaggc gaatttgagc gaggtatcct tgcgcttccg cgatgtagtg
     1621 ccctacggca cccagctcgt cacggactgc gatctcgcgc gcttgatcca gggaggcttc
     1681 aaggccttcg atgcgaaacg cagcacacgc ggcaaaggca tttcgctttt gaacgtcgtc
     1741 gctggtcgtc atggctccat cctcacgttt gccggaacct agcgtcgcgc gggggcttcg
     1801 atcttttgct ttgccccctc catgtcgcca cggcctcggc ggattgctga cgtgacctgt
     1861 gacctgtctt gggctggtgg catagtcctc cagagttggc ttaaggtcct tgggctaaat
     1921 ctgaatcgtt ctgaatcgcc ctaaatatct tcgcacgggc ggtcgg
//
LOCUS       DATLJT010001158         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2380580,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001158 DATLJT010000000
VERSION     DATLJT010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380580"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VM910_26730"
     CDS             <1..770
                     /locus_tag="VM910_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI66150.1"
                     /translation="RHGDHTSGLNHLLKVNPAVKIYVPQENFGVFGAALPGTFYRRNE
                     SLLSDMRYFDGRPPETLRFGSPWPEGNFTWITKTTEVAPGFHLILLNGTWGVDLEVKE
                     ISLAIDTPDGIVLVVGCSHPTIEKIVEAAKTAINKPIHLVLGGTHLLPAKDDQINSIA
                     VSLRDNWSVRYLAPVHCTGEPAFAILKETFGDRYIYAGLGTTVLLGPKVTVKAEAGQP
                     YKNAMDEEDLRSYREAMMRGPLRGLLGGDKRLTRVGQ"
     gene            1063..1221
                     /locus_tag="VM910_26735"
     CDS             1063..1221
                     /locus_tag="VM910_26735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66151.1"
                     /translation="MSKLFAAIAITASLAAAGSAQAQTYPSRPITIVVPFPAGGPTDA
                     LARVLANG"
     gene            1218..2036
                     /locus_tag="VM910_26740"
     CDS             1218..2036
                     /locus_tag="VM910_26740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66152.1"
                     /translation="MKAALGQSVIVENPTGAAGTIGTGRVARAAPDGYTVILGHWQTH
                     VVNGATYALPFDVVKDFEPISLVADCPVWFVAKAALPPKDLRELVVWMKENPGKATVG
                     IGGVGGGADVVGTYFQNNTGTRFQFVPYRGAAPMIQDLVAGQIDLTFTQVASALAQVR
                     AGQLKAYAVMAKTRWAAAPDTPTFDEAGVPGLHASFWHGLWAPKGTPKDVIANLHAAL
                     LETLADPSVRQRLTDLGQGIWPRDKQTPEALAAQQKAEIEKWWPIIKAANIKAE"
BASE COUNT          401 a          660 c          706 g          394 t
ORIGIN      
        1 atcgccacgg cgaccacacg agcgggttaa atcatctttt gaaggtcaac cccgcggtga
       61 agatctatgt gccgcaggaa aacttcggtg tgttcggtgc ggccttgcca ggcactttct
      121 acaggcgaaa cgaatccctg ctttcggaca tgcgttattt cgacgggaga ccgccggaga
      181 cgctgcgttt cggcagcccg tggcctgaag gtaatttcac atggataacc aagacgaccg
      241 aagtggcgcc cggattccat ttaatcctgc tgaacgggac ttggggcgtc gacctggagg
      301 tgaaggaaat ctcgttggcg atcgacacgc ccgacggcat tgttctcgtt gtcgggtgca
      361 gtcatccaac aatcgaaaag atcgtcgagg cggccaagac tgcaatcaat aagccgatac
      421 atctggtctt gggaggcacg catttattgc cggcgaaaga cgaccagatc aacagcatcg
      481 cggtctctct acgcgacaac tggagtgtgc gctatctcgc tccggttcac tgcactggcg
      541 agcccgcctt cgccattctc aaagagactt tcggtgaccg ctacatttat gccggcctcg
      601 gcacgaccgt tttactgggg ccgaaagtca cggtgaaagc cgaagcggga cagccgtaca
      661 agaacgcaat ggacgaagag gacttgcgca gctaccgcga ggcgatgatg cgcggtccct
      721 tgcgcggctt gctcggggga gacaagcgtc tcacacgcgt ggggcaatga ccgttcgccg
      781 ccggtcgaac ctgcgtcgcg cgagagattg gggccggggc atcggcttct gaagcgttgc
      841 ggcgcaggcc gtcatacccc ttccgccggc catcgaggtc gcatagcctg tcaagcaccg
      901 tcctgcggga tgtgccgaag tctccgcggc ggaacacaca cagcgctggt gccgtcggca
      961 aacacgctct ctcaagggac cagtttcttt ttttttggtc tcacgtgggt cactcccatt
     1021 tgcatagtcg cgcgccgctt ccgtcgcggc gggaggtgag agatgagcaa gctctttgct
     1081 gctattgcca tcactgcatc gttggccgcc gctggcagcg cgcaggcgca gacctatccc
     1141 tcgcgtccta tcaccattgt cgtgccgttc ccggcgggcg ggccgaccga cgcgctggcg
     1201 cgcgtgctcg ccaacggatg aaggcggcgc tcggccaatc cgtcatcgtc gaaaacccga
     1261 ccggggcggc aggcaccatc ggcaccggcc gcgtcgcgcg cgccgcgccc gatggctaca
     1321 cggtcatcct cggccactgg cagacgcacg tcgtcaacgg cgcgacctac gcgctcccgt
     1381 tcgacgtggt gaaggatttc gagccgatat cgctggtggc ggactgtccg gtgtggttcg
     1441 ttgcaaaagc tgctttgccg ccgaaggatc tgcgggaact ggtcgtttgg atgaaggaaa
     1501 atccgggcaa ggcgacggtg ggaatcggcg gcgtcggcgg cggggcggac gtcgtcggga
     1561 cctactttca gaacaatacc ggcacccgct tccagttcgt gccctaccgc ggcgccgcgc
     1621 cgatgatcca ggacctggtg gccgggcaga tcgacctgac cttcacccag gtggcgagtg
     1681 cgctggcgca agtgcgcgcc ggtcagctca aggcctatgc ggtcatggcc aagacccggt
     1741 gggcggcggc gcccgatacg ccgacgttcg acgaagcggg agtgccggga ctgcacgcgt
     1801 cgttctggca cggcctgtgg gcgcccaagg gcacgcccaa ggacgtcatc gccaaccttc
     1861 atgccgcgct gttggagacc ttggccgatc cctcggtgcg ccagcggctt accgacttgg
     1921 gtcagggaat ctggccgcgc gacaaacaga cgccggaggc gcttgccgcg cagcagaaag
     1981 ccgagatcga gaagtggtgg ccgatcatca aggcggcgaa catcaaggcg gagtgagtcc
     2041 tcggctggct gttgattcgg tcgtggcgcg gctcgcaagt gcgacagcgc gatgaccatg
     2101 cgccgacggt ggtgcggggg tattcccgtc acgaatgcga aatcgctacc gatatggccg
     2161 c
//
LOCUS       DATLJT010001159         1559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2151075,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001159 DATLJT010000000
VERSION     DATLJT010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151075"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VM910_26745"
     CDS             <1..373
                     /locus_tag="VM910_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HVI66153.1"
                     /translation="MLTQLRDVLDSFACRDVAKAVEVWTRDQDIDRLCTSLSQEFLAY
                     MMDKSVAMSFGAHLMFCAKNLERMGDHATNIAEAVHYMVKGETLLRERPKADFTSMLT
                     VAMGRDITALAPVCTESSVLS"
     gene            489..893
                     /locus_tag="VM910_26750"
     CDS             489..893
                     /locus_tag="VM910_26750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66154.1"
                     /translation="MTSHPIRAITLTAAFAITSASAAAPTHAAPFDGDWSVLVHTTDG
                     HCGTTRWGLAIRGGQVYYAGGYAYGHLVGLAGPPPVISGSTSWPAHAAPMAPDGFGKV
                     RAAEVGRVAVRREPARASGAPLEVNCRSAGAQ"
     gene            1067..>1559
                     /locus_tag="VM910_26755"
     CDS             1067..>1559
                     /locus_tag="VM910_26755"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HVI66155.1"
                     /translation="MSPAREIELKLEVPAHSLYRLARSPLLRAARKKSSKPATLVSVY
                     FDTDKLKLRNKGLSLRVRRIGRRHIQTIKQESGESAALFTRDEWEHQIGSRQPDLDVA
                     QDAALGPVFGKKVRRSLKPIFETRVRRTVYPIQSGVSEIELTVDKGKVEAGRQSSPLC
                     EVEL"
BASE COUNT          321 a          482 c          479 g          277 t
ORIGIN      
        1 catgctgact cagttgcggg atgtccttga cagtttcgcg tgccgcgatg tcgcgaaggc
       61 ggtcgaggtt tggacgcggg accaagacat tgaccggctg tgcacaagtt tatcgcagga
      121 gtttctcgcg tacatgatgg acaagtccgt tgcgatgtcc ttcggcgctc acctgatgtt
      181 ttgtgcaaag aatcttgagc gcatgggcga tcatgcgacc aatatcgcgg aagccgttca
      241 ctacatggtg aagggcgaga cgcttttgag agagcgacca aaagcagatt tcaccagcat
      301 gctcaccgtg gcgatggggc gggacataac agctctggct cctgtgtgta ccgagagctc
      361 cgtcctttcg tagcccggac gctaacaacc ccgcaccgag tgtgagaaaa tacggcccat
      421 cgctcgaccc cacgatcgcg gccagagcaa cgaccagcta ggcgcctcca cgccaacagg
      481 aggagcccat gaccagccat cccatccgcg ccatcactct gacggccgct ttcgcaatca
      541 caagcgcctc tgccgctgcg cccacacatg cggcgccgtt tgacggagat tggagcgtac
      601 tcgtccacac caccgacggc cattgcggaa ctacgcgctg gggcctcgcg atccgcggcg
      661 gccaagtcta ctatgcgggc ggctatgcct atggccacct cgtgggcctc gcaggtcccc
      721 ctccggtcat atccgggtca acatcctggc cggcccacgc agcgcccatg gcgccggacg
      781 gctttggaaa ggtcagggca gcggaagttg ggcgggtcgc ggtccgtcgg gaacctgcgc
      841 gggcgtctgg agcgccgctc gaggttaact gccgctcggc aggcgcacag taaacaccga
      901 gccgtaatcg gcgatccaag cgaacgattg ctcgaaacgg ctcgacgttg tgcgcagcgg
      961 gcagagccgc agccgtcacg agtatccagg tctcacggcc cccctagaaa ttgcgggcga
     1021 aatcggcaat tatattgaat agatacccat ttcagctgat tttcccatgt cgcctgcgcg
     1081 tgaaatcgag ctcaagctcg aagtgccggc ccatagcctc tatcggctgg ctcgcagccc
     1141 gttgctaagg gcggcgcgga agaagtcgtc taagccagca acgctcgtgt cggtctattt
     1201 cgacaccgac aagttgaagc tgcgcaacaa gggtttatcg ctgcgagtgc ggcgcatcgg
     1261 ccgccgccac attcaaacca tcaagcagga aagcggcgaa agcgccgcgc tatttacccg
     1321 cgacgaatgg gagcaccaga tcggcagcag gcagcccgac ctcgacgtcg cgcaggacgc
     1381 cgcgctcgga cccgtgttcg gcaagaaggt gcggcgcagc ttgaagccga tattcgagac
     1441 gcgcgtgcgc cgcacggtct atccgatcca aagtggagtt agcgagatcg aactcaccgt
     1501 cgacaagggg aaggtcgagg cgggtcgcca gtcttcgccc ctttgcgagg tggaattgg
//
LOCUS       DATLJT010001160         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1922205,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001160 DATLJT010000000
VERSION     DATLJT010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922205"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VM910_26760"
     CDS             <1..245
                     /locus_tag="VM910_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66156.1"
                     /translation="RASQYDNRRNHPQPSRHRVSPQLPPPGAIVTSIKGHKGGVSDAV
                     KIFNRSNMLSIFKGYFFAFSALWAYPGHAFCTGEIG"
     gene            263..937
                     /locus_tag="VM910_26765"
     CDS             263..937
                     /locus_tag="VM910_26765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66157.1"
                     /translation="MQHRTRDLLMRQRNQVINVSLPNRRGGTPKVAPEPHPPSAEIAL
                     GERGEGDTARRFVRGSFFVCLPFLLIGKGAAVTTAGFALTLVFIGSAAGKLACGWIGN
                     WLGNRATIAICQSLTAVGIVVVLLLPVQLTLVMLPFVGLVLNGVTTVIYGSVPSYAAP
                     ERRTHLLSVFYTITIGAAAVSPPAAGFLSDLIGIPGTVIVVSALTLATIPCAFLLKDL
                     RRTASV"
     gene            2282..>2639
                     /locus_tag="VM910_26770"
     CDS             2282..>2639
                     /locus_tag="VM910_26770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66158.1"
                     /translation="MGLKIHCLGDGSQFSTLVEALGGAQKILDVNYYKHSHASLCLVL
                     PPVGSARSAILLLECIEHFIGSALFNNPEIQIQVCSPGRLGARRSALLAIGFYLGSDT
                     LRRYTLGDLATSFAENQ"
BASE COUNT          490 a          799 c          790 g          560 t
ORIGIN      
        1 gacgtgccag ccaatatgac aacaggcgca accaccccca accatctcga caccgcgttt
       61 ctccgcagct tccgccccca ggtgctattg ttacatccat aaagggacat aaaggtggcg
      121 tttcagacgc ggtgaaaatt ttcaaccggt ccaatatgtt gagcatcttc aagggatatt
      181 ttttcgcatt ctccgcgttg tgggcttatc caggccatgc gttttgtacg ggtgaaatcg
      241 gctgagcagc aggggtcagt tgatgcagca tcgcacgcgc gatctgctga tgcggcaacg
      301 gaaccaggtg atcaatgtgt cgcttccgaa tcgtcgcggc gggaccccta aggtcgcgcc
      361 ggagccgcac ccgccctccg ccgagattgc cctcggcgag cgcggcgaag gcgatactgc
      421 gcggcggttc gtgcgaggca gcttttttgt gtgcctccca ttcctcctga tcggaaaggg
      481 ggcggctgtc acgaccgcgg gattcgcatt gacgctggtc tttatcggca gcgccgccgg
      541 aaagcttgcc tgcggatgga tcggcaattg gctcggcaac cgcgccacca tcgccatttg
      601 ccagtcgctc accgcagtag gaattgtcgt ggtcttgctg ctgccggtcc aacttacact
      661 ggtcatgctt ccgttcgtcg gtctggtgct caatggcgtc accaccgtga tttacggctc
      721 tgtgccgagc tatgctgcgc cagagcggcg cacacatttg ctcagcgtgt tctacacgat
      781 cacgatcggc gcggccgccg tttcgccgcc cgctgcggga ttcctcagcg acctcatcgg
      841 aattcccggc acggtcatcg tcgtgtccgc gctgacgctg gccacgattc catgcgcgtt
      901 tctgctcaag gacctgcgcc gcacggcctc cgtctaaggc cggccggatt tagccggccg
      961 acctgccggt gcaggggtgg tcgagtgacg ctcgaggcgc gcagcacccg cgcctcaacg
     1021 aatttctcag atgctcgcgg tcgaggtcgg cccgcgcggg acggccgctc aaaggcggtc
     1081 aaccttcgct tgcttacccc ggagcgttag aaacgtctca ctcccgtcgc caggctcctt
     1141 cgaacgcggc catggcggcc tcggctgtag ggtgcgagaa ggaaggcact attgcgagta
     1201 ccggtcgcag tagtctttcc cggtttccat ttccacctta gtaatggtga cgtactcgac
     1261 gtctgaacac ccacgcgtcc acttgtccca ttgccgcttt ttaccgaaat cgcgggcgca
     1321 tgcctccgcg ccagcaaaca tctgagctga agcacgctgc tgtcgtcggc taaccctcga
     1381 cggtcacggc ggcagcgagg gcgtggcgct caggtgcaac gcgggttgcc gaattgatgc
     1441 cgggactggc cgctcgctgt ggccaagtgt ggattaagcg aacggccgac cagtgtgccc
     1501 gcgaatcagc ggccgctcgc gccagcctgg gcagaacggg gccctccggc ggtcttgcgt
     1561 ttcggggatc caagaaccat ttaatggcac ataaaaggcg gcgcttgacg acgcctcccc
     1621 aaacacaagc gacaccaaag ctcgggccgc aagggctggg ccacgtcaca tctatgagac
     1681 caaacatgac tgacgctgcc accgttaaga tgtcgcctcg ctcgcgcaag gccgacgacc
     1741 gttggatgag atccatactc cgaggccaga agttcgaggg gacgcggctt ttcgaagcgc
     1801 acggtcgatg tgcttctcgc gcacggcatc agcgcgcccg agcggctgtt gttcatgtct
     1861 gagaccgagc tacagaacat acccggcatt ggtgcgattt cactcgggga gattttgcgt
     1921 tatcgcaagc aacatctctc gggagcgtaa ccaacgcgcg cgctctttgc tggcgctccc
     1981 aaggtaatga gggatgcccg gcgcaagcgg ttgcgcttct tgtttgatgc acgactcatg
     2041 ggagtgcgga agcgcgcaca atcgtgagag cgggcgtata cgctcacgga cagcggacgg
     2101 gcgaaggaca tccttagccg catttacgaa taggcaaggt gctacttcat ccgaaagagg
     2161 tgcccctgtg tcttaccggt acagtttcga agatctgttg gcgcttttgc acgggcacgc
     2221 tccggcgaag gttgacgcgg tcgcactcca tcgtcggcgc gttgagcatg gccatctgtc
     2281 agtgggactc aagatccact gtctgggcga tgggagtcag ttctctactc tggtggaagc
     2341 tcttggagga gcgcaaaaaa ttctcgatgt caattactat aaacactccc acgcctcgct
     2401 gtgtcttgtg ctgcccccgg tgggcagtgc tcgttcggcg atactcctgc ttgagtgtat
     2461 cgagcacttt attggatctg cgcttttcaa taacccggaa atccagatcc aggtatgctc
     2521 tccaggaaga cttggcgctc gtcgttcggc attgttggcg atagggttct acttggggtc
     2581 ggatacgctg cggcgctaca ctctgggaga cctcgcgaca tcctttgcgg aaaaccaac
//
LOCUS       DATLJT010001161         4205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1462973,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001161 DATLJT010000000
VERSION     DATLJT010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462973"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VM910_26775"
     CDS             complement(<1..351)
                     /locus_tag="VM910_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVI66159.1"
                     /translation="MFKRGIEGFPYYLGVGALDRIATREDRVCVLNILGGESRQVTPV
                     SHAFSGGNVVFGTSPGRRGQVLPTPAGDVPVYNNVREGLDDGHAFNTGVVYLPPSGVR
                     DGVAELIRVNPGLEK"
     gene            complement(557..1837)
                     /locus_tag="VM910_26780"
     CDS             complement(557..1837)
                     /locus_tag="VM910_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVI66160.1"
                     /translation="MNITGMLYGAPLLKHVDFPTSEVLGPEATEDEIQGLIDRHKLIL
                     IKPIFRGGVGKKGKAGLIGRASDLKTALKEKERLYFIEHQHGNARAKANGVTFESGIP
                     AEHEVYFAITDSTHYRAPTMTLTHRGGVDIEEIDKREVAQVPFEALTGLKAFVVANAL
                     SDIGAPKEIISPLVQQLPKLWELVHHYGMTTLELNPIRMRPGREGRLTPIACDFKCGF
                     DRDDPRWQRLDLPDHLFVADASEFEHEVNQLRTHQGQSDVFVINEQGTILAPTFGGGA
                     NSLVTEVLGEAAIISSDFGGNPPYEKMKAVARICYKHWLKQANVLFIIGGKSNNTDIY
                     ETFRAMGDALREYFSSNGPTPLYVVVGRGGPNLVRGMGALAETCDSLNLPYRFFGFDS
                     AISEVVNYVKDIDEWMKAGGRAEIAAHLGLKAGN"
     gene            complement(1849..3204)
                     /locus_tag="VM910_26785"
     CDS             complement(1849..3204)
                     /locus_tag="VM910_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HVI66161.1"
                     /translation="MQQSVSVSASPEALHAGWWPQGWWKIMALRIGIVPIPIYVILVA
                     VLAVFTYTGDIKGEVSTMIAVLVIGGFTCAEIGKRIPVLRNIGAAAIFATFVPSALAY
                     YKLLPPQIEKSVVEFTKYTNFLYLFIACIIVGSILGMDRKVLVQGFLKIFVPLAVGSI
                     AAGIVGTFVGTLLGMGASHTFFFVVIPIMAGGVGEGAIPLSVGYSEILGQPQGDMFAQ
                     VLPPVMLGSLTAILLAGTLNYVGKQYPNLTGEGRLQPGEHDEMDPAREDAEDEIGAPI
                     DVGTIAAAGLAAITLYLLGVMCYRLFGLPAPVAMLFLAVIAKLVSAVPPHLQRGGFVV
                     YKFFSTAVTYPLLFAIGVSMTPWDKLIAAFNLPNLITIISTVATLMATGGLIGRWLNM
                     YPIEVAIINACHSGQGGTGDVAILTAANRMQLMPFAQIATRIGGAITVTLVLITLSRL
                     V"
     gene            complement(3267..>4205)
                     /locus_tag="VM910_26790"
     CDS             complement(3267..>4205)
                     /locus_tag="VM910_26790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66162.1"
                     /translation="ERRGHLRDYGLAVAMTDFEGGGESLDALLAGSVDVAVGAYEHTL
                     RAQGMGKDIRAAIELGRFPGIVLAVGRDRTFKSLADLKGLKIGVTALNSSSYFFVLYL
                     MAKAGLAPGDASFLSVGGGQRAVDAIKAGEIDAISNLDPVITRLQQDGAIRILVDSRF
                     PRVNYDIFGGTNPAAVLYAKQEFIAANPATMQALVNAFYKTLRWIATATTDEIVDAVP
                     ADYFLGERAIYVKALKANLLVYSRTGIITRQGMNSALKMMATFDPDLKDKKIDLQKTF
                     DDRFVKRAAVLFDDPTNSDLELDERRPEIDLGSFRD"
BASE COUNT          822 a         1276 c         1295 g          812 t
ORIGIN      
        1 cttttccagg cccgggttga cgcggatcaa ctcggcgacc ccgtcacgca cgccggaggg
       61 cggcaggtac acgacaccgg tgttgaacgc gtgcccgtca tcaagccctt cgcgcacatt
      121 attgtagacg ggaacatcgc cagccggcgt cggcagcacc tggccgcgcc gacccggcga
      181 agtcccgaac accacgttcc cgccggaaaa tgcgtggctg accggcgtca cctgtcgcga
      241 ttcgccgccg agaatgttga ggacgcagac ccgatcttcg cgcgtggcga ttctgtcgag
      301 cgcgccgacc ccgagatagt agggaaaacc ctcgatgcct cgcttgaaca tgcccgcctc
      361 ctgccggcag cggtcctctg ctctagtgtt ctgtcaccga cccttgatcc tgcgcgttgc
      421 gcggctgtgg cccagcggat ctcgcttgcg ctgtcgtgcc cctcttcgag atggccggga
      481 caatagagtc cccgccgaca aagtcggcgg gtgcctcgac ccgtaacgaa ttgactctct
      541 ccgagcgaaa catgctctaa ttacccgctt tcaatccgag atgtgccgcg atctcggccc
      601 tgccgccggc cttcatccat tcatcgatgt ctttgacgta gttgacgact tccgagatgg
      661 cgctgtcgaa gccgaagaag cgatagggca gattgagtga gtcgcaggtc tcggccagtg
      721 cgcccatgcc gcggacgaga ttgggtccgc cgcgtcccac aacgacatag agcggggtgg
      781 ggccattgct gctgaaatac tcccgcagcg catcgcccat cgcccggaac gtctcgtaga
      841 tgtcggtgtt gttcgacttg ccgccgatga tgaaaaggac gttcgcctgc ttgagccagt
      901 gcttgtagca gatgcgcgcc accgccttca ttttttcgta gggcgggttg ccgccgaaat
      961 cggacgaaat gatcgcggcc tcgcccagca cctccgtcac cagcgaattg gcgccgccgc
     1021 cgaaggtcgg cgccagaatc gtaccctgct cgttgatgac gaagacgtcg gactggccct
     1081 ggtgggtgcg cagctggttg acctcgtgct cgaactccga ggcatcggcg acgaacaggt
     1141 gatccggcag atccaggcgc tgccagcgcg ggtcgtcgcg atcgaagcca cacttgaaat
     1201 cgcaggcgat cggggtgagc cggccctcgc gaccgggccg catgcggatc ggattgagtt
     1261 cgagcgtggt catgccgtag tggtggacga gctcccagag cttcggtagc tgctgcacca
     1321 gcggcgagat gatttccttg ggggcgccaa tgtcgctgag ggcgttggcg acgacgaagg
     1381 ccttgagccc ggtcagggct tcgaacggca cctgcgcgac ctcgcgcttg tcgatctcct
     1441 cgatgtcgac gccgccgcga tgggtgagcg tcatcgtcgg cgcgcggtag tgcgtcgaat
     1501 cggtgatcgc gaaatagacc tcgtgttcgg cggggattcc gctctcgaaa gtgacgccgt
     1561 tggccttggc tcgcgcgttg ccgtgctggt gctcgatgaa ataaagccgc tccttctcct
     1621 tgagcgcggt cttgaggtcg ctcgcgcggc cgatcaggcc ggccttgccc ttcttgccga
     1681 cgccaccgcg aaatatcggc tttatgagaa tgagcttatg gcggtcgatg agaccttgga
     1741 tctcgtcctc tgtcgcctcc ggtccgagta cttcggatgt gggaaagtcc acgtgcttga
     1801 gcagcggcgc cccataaagc ataccggtga tgttcatggt cgtgctccct aaaccaggcg
     1861 cgacaacgtg atcagcacca gcgtgacggt aattgcgccg ccgatacggg ttgcgatctg
     1921 cgcaaacggc atgagttgca tccgatttgc agccgtgaga atggcaacgt caccggtgcc
     1981 gccctgcccg ctgtgacaag cgttaataat ggcgacctcg atcgggtaca tgttgagcca
     2041 gcgaccgatg agccctccag tggccatgag tgtcgccacc gtgctgatga tggtgatgag
     2101 gttcggtagg ttgaaggcgg caatcagctt gtcccatggc gtcattgaca cgccgatggc
     2161 aaacaacagc ggatacgtca cggcggtcga gaagaactta tagacgacga acccgccccg
     2221 ctgcaggtgc ggtgggaccg cgcttaccag cttcgcgatc acggcaagga acaacatggc
     2281 gactggggca ggcaatccga acaaccggta acacatgacg ccgaggagat agagcgtgat
     2341 cgccgcgagg cccgcggccg cgatggtgcc gacatcgatc ggggcgccaa tctcgtcctc
     2401 ggcatcctcc ctggcgggat ccatttcgtc gtgttcgccg ggctggagcc gcccttcgcc
     2461 cgtcaggttc ggatactgct taccgacgta gttgagcgtg cccgcaagca ggatcgccgt
     2521 gaggctgccg agcatcaccg gcggcagcac ttgggcgaac atgtcgccct ggggctggcc
     2581 gagaatttcc gaatagccga ccgagagggg aatcgcgccc tcaccgacac cgccggccat
     2641 gatgggaatt acaacaaaga agaaggtatg agaggcgccc atgccgagca aggtgccgac
     2701 aaacgtgccg acgattccgg cggcgatcga gccgaccgcc agcggcacga atattttcag
     2761 aaatccttgc accaggacct tgcggtccat acccagaatg ctgccgacga tgatgcaggc
     2821 gatgaacagg tagagaaagt tggtatactt ggtgaattca acgaccgatt tctcgatctg
     2881 cgggggcagc agcttgtaat acgcgagcgc cgagggaacg aaggtagcaa aaattgcggc
     2941 cgccccgatg ttgcgcagca cgggtatgcg cttgccgatc tccgcgcagg tgaagccgcc
     3001 gatcaccagc acggcgatca tggtggagac ctcgccctta atgtcgccag tgtaagtaaa
     3061 aactgccaaa actgcgacga ggatcacgta gatcgggatc ggaacgatcc cgatccggag
     3121 ggccatgatc ttccaccacc cttgcggcca ccaccccgcg tgtaacgctt ccggggatgc
     3181 cgagacggag acgctctgct gcatggtccg ctccaactct gctcttcagt gaagaaccag
     3241 aagagcttgc tgtcgaattc ccatgatcag tcgcggaacg agccaaggtc gatttcgggg
     3301 cgacgctcgt ccagctccag atcgctgttg gtcggatcgt cgaacagtac tgccgcgcgc
     3361 ttgacgaacc gatcgtcaaa cgtcttttgc agatcgatct tcttgtcctt gagatcggga
     3421 tcaaacgtcg ccatcatctt gagcgcgctg ttcattccct ggcgagtgat gattccggtc
     3481 ctggaataga ccagcaggtt ggctttcagc gctttaacgt aaatcgctcg ctcgccgaga
     3541 aagtaatcag cgggcacggc atccacgatt tcatccgtgg ttgcggtcgc aatccatcgc
     3601 agagtcttat aaaaagcgtt gacaagcgct tgcattgtcg ccgggttcgc ggcaatgaac
     3661 tcttgcttgg catagagtac ggccgctggg ttagtgcccc cgaagatgtc gtaattaact
     3721 cgcgggaatc ggctatcgac gaggattcgt atcgccccgt cctgctgcag gcgagtgatc
     3781 accggatcga gattggagat cgcgtcgatc tcgcccgcct ttatcgcatc cacggcccgc
     3841 tgcccaccac ccacgctgag aaacgatgca tcgcccggcg caaggccagc cttcgccatc
     3901 aggtagagca caaagaagta gctggaagag tttagggcgg tcacgccaat cttgaggccc
     3961 ttgaggtcag caagtgactt gaaagttcgg tccctgccga cagcgagcac gatgccgggg
     4021 aaccggccga gctcgatcgc ggcgcggatg tcctttccca tgccctgcgc acgtaatgta
     4081 tgctcgtatg cgcctactgc gacatcgacc gagccggcga gcagggcatc gagcgattcc
     4141 ccgccgcctt cgaaatcggt cattgcgacg gcaagtccgt agtcgcggag gtggccgcgc
     4201 ctttc
//
LOCUS       DATLJT010001162         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1463078,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001162 DATLJT010000000
VERSION     DATLJT010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463078"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1518)
                     /locus_tag="VM910_26795"
     CDS             complement(142..1518)
                     /locus_tag="VM910_26795"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI66163.1"
                     /translation="MPRFSDLGLSPPILGALAAEGYAAPTPIQSQAIPPVLRGRDLCG
                     IAQTGTGKTAAFALPILQRLAAAPQRAAPHTCRVLALSPTRELASQIAVSFRAYGAGM
                     PLSTAVVFGGVPIGPQRQKLARGIDILVATPGRLLDLIDTRSLTLSSVHVLVLDEADR
                     MLDLGFIHALKRIVKLLPQQRQTLLFSATMPRPIALLAEDYLTDPIKVVVAPSSTKVD
                     RVEQGVVFVPSDRKRDLLATLLRDPVLARVLVFTRTKHGADRVVRHLVSAGIEATAIH
                     GNKSQPQRERALAGFRDGQTRVLVATDIAARGIDVDGVSHVNFELPNVPEDYVHRIGR
                     TARAGAAGTAIAFCSDEERPYLRDIEKLTRCSLRAIPLAIPAPGIGAIERLGKATLPW
                     DASLASRQATARGRDAPARHPPRPAGADVRHRDPPVTGSFPAFLRRPSSTKRSSVGRR
                     SERRGRSV"
BASE COUNT          451 a          731 c          798 g          444 t
ORIGIN      
        1 gcaaagtcgc cgtcagtgat ctggtcattc gcgtcgcgcg tcatgcgacc gcagactgta
       61 cccttcggac agacgatctc ctcgccttcg cgcgcaatca gcatgtctcg ggtcctcgct
      121 gcatgcgttt accgcgcacc atcaaaccga gcggccccgc cgttcgctgc gacggccgac
      181 cgaactacgt ttggttgacg acgggcggcg caggaatgcc ggaaaggacc ctgtaactgg
      241 tgggtctcgg tgccggacgt cggcgccggc aggccgcggt gggtgtcgcg ccggggcgtc
      301 tcggccgcgc gcggtcgcct gacgtgacgc gagactggcg tcccagggca acgttgcttt
      361 ccccagccgt tcgatagcgc cgatgcccgg cgccggaatt gccaacggga tcgcgcgaag
      421 cgagcatcgc gtcagcttct cgatgtcgcg cagatagggc cgctcctcat cgctgcagaa
      481 cgcgatcgcg gtccctgcgg cgccggcccg ggcggttcgg ccgatgcgat gcacgtaatc
      541 ttcgggcacg ttcggcaatt cgaaattgac gtgcgacact ccatcgacat cgatgccgcg
      601 ggcggcaatg tcggtcgcga caagcacgcg ggtttggccg tcacgaaagc cggcgagcgc
      661 tcgttcgcgc tgcggctggg atttgttgcc gtgaatggcg gtcgcctcga tgccggcgct
      721 gaccaggtga cgcacgacgc ggtcggcgcc gtgcttagtt cgggtgaaga cgagcacgcg
      781 cgcgaggacc ggatcgcgca gcaaggtcgc gagcaaatcc cgcttgcgat cgctcggcac
      841 aaagaccacc ccctgttcga cgcgatcaac cttcgtcgac gacggtgcga cgactacctt
      901 gatcgggtcg gtgagatagt cttcggccag cagggcgatg ggccgcggca tcgtcgccga
      961 gaacagcagc gtctgccgct gttgcggcaa cagcttgacg atgcgcttca gcgcatggat
     1021 gaagccgagg tcgagcatcc ggtcggcctc gtcgagaacc aggacatgca cgctcgacag
     1081 ggtcagcgac cgggtgtcga tcagatcgag caagcgaccc ggtgtggcaa cgaggatgtc
     1141 gatgccgcgc gctagtttct ggcgctgcgg cccgatcggg acgccgccaa agaccaccgc
     1201 cgtcgacagc ggcatcccag cgccgtaagc gcgaaagcta accgcgatct ggctcgccaa
     1261 ttcacgcgtc gggctcagcg cgaggacgcg acaggtgtgc ggtgcagctc gttgtggagc
     1321 ggcggcgagg cgctggagga tcggtaatgc aaaagcggcg gtctttccgg tgccggtttg
     1381 tgcgatgccg cacagatcgc gcccgcggag caccggcggg atggcctgcg attggatcgg
     1441 cgtcggtgcc gcgtagcctt cggcggcgag cgcgccaaga ataggtgggg aaagtccaag
     1501 gtcggagaaa cggggcaagt tgataacggc ctctcggtat cggcatgccg acagtacgtt
     1561 cacgtggacg cactgcgcgg tcgggagacg acccgcgcga ttagggctgt ccagatgtcg
     1621 gcagggataa agatcggtcc gcttccaccg cgtcggtggt tcgctcacgc aggcccgatc
     1681 acgtgactca acccaggagc acgaacactg cacggctaat atgctggcgg atccggcggg
     1741 atcaagtgcc tgcgacattc tcggccgcta gagtcgcggc cggcaggcgg atgatcgtct
     1801 tctccgggca tatcgccaat tgggagatcg caatgcttgc cggcatctag cacgggatct
     1861 ctgtggccca gatctatcgc atcggcaaca atccgctgct aggccgcatg acgcgaccga
     1921 tagagaccgt ttcgtatcca cgagagaccc cgtgaaaaat gatgtaaata aaacaagctt
     1981 tctctgccga tttctcttag aagaggcaaa tttacctcag agacatcggg ctttgttacg
     2041 cgattgaaat cggttgtata attgaagatt gaccccattt aagcgtctac cggcatttca
     2101 gcgactagct gaacacgcgc aaatcgtatg ttgattcact cctcccgggg acgctagcag
     2161 ttgatgacca tgacccaaga acgggacctt gttgaccacg ctccagcgcc tccgcgatga
     2221 cgttgggcgg atctttccgc ccgttgcggc cggatctcga tagatttttg tttgcgacgg
     2281 tcggagacga gatcgatgga gttccgttga gcgtggtctc tgccttgacc cgcctcggcc
     2341 ttgatccttg gcaggaggct ggacggctgt cgtcgctgag caaccgcgag gcgatcgagc
     2401 aactggcgcg gctaatcgca gaac
//
LOCUS       DATLJT010001163         4242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3071250,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001163 DATLJT010000000
VERSION     DATLJT010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071250"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VM910_26800"
     CDS             complement(<1..696)
                     /locus_tag="VM910_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66164.1"
                     /translation="MTNLSNVVALPFAEIWAEDFEWVPKLGERHDVVCYGARELRSGR
                     TLRLWRDQLGPTSPNRTDDQALFLSFVFNAEGASRLSLGWQLPAKVIDLSPLFRRVAN
                     GRIVPQGKGLLGALAYYGIPSIDGVYKEKIRKLIMRGWPFTPEEKAEILKYVMTDVDP
                     LFELFSQLMAEPEFDLGTALHWGEFVAVSAQMEHRGVPLDMEIIPDLLDKKAWAYVRD
                     AVVPKINAQYDVYV"
     gene            complement(680..1300)
                     /locus_tag="VM910_26805"
     CDS             complement(680..1300)
                     /locus_tag="VM910_26805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66165.1"
                     /translation="MADETSKPKLAVVTEDLDEDEIEFKKLRRDFPGVKGASAIGIVS
                     ITVSRKPEENEFFRTHPDFMPIIPMVNIEVGMERQFFAVTDNMVAALAGIGITVSDHR
                     LYMIVTPRGAVRTIPINVESDNEYIRSKEIGLIEGTRQWVRLYSDRENRNYKVFPAPV
                     GRFSDPIWPELKHAKIFRVAFRDKGRLVDSPDHPLFLKWAARDKSK"
     gene            complement(1401..1637)
                     /locus_tag="VM910_26810"
     CDS             complement(1401..1637)
                     /locus_tag="VM910_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66166.1"
                     /translation="MEATTMARGEITGKKPRQTPPPAAYTIAEFCLSHRIAVSMYYKL
                     KTQGLGPREMAVGSRRLISQEAAAEWRKAREAAA"
     gene            complement(1739..2290)
                     /locus_tag="VM910_26815"
     CDS             complement(1739..2290)
                     /locus_tag="VM910_26815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66167.1"
                     /translation="MKAKALPRRGKKVSIDKAFRKLRPALGPNGAVDEMNKALLRDDK
                     RARLFSNGRLVNPNFIRTNLVVKGPGSRRADGQRRRRAEIAAKRALDKPEEAYTWEMD
                     ADEMIEALLPTTSGKSPRKTSAKRKRTRQQELVLEVAAEIWPGGWEHIETREIIKKVS
                     DKLEARSLAVPKRDVFLRALDRR"
     gene            complement(2287..2568)
                     /locus_tag="VM910_26820"
     CDS             complement(2287..2568)
                     /locus_tag="VM910_26820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66168.1"
                     /translation="MKIKKILLAFALVLAATATAQAQDRQQTRFYEPNGQSIGTAVPL
                     GDASVRYYDARGHSLATSTTTGNTTTFYDERGRPTGSFQFDGRMRRPAR"
     gene            complement(2565..3818)
                     /locus_tag="VM910_26825"
     CDS             complement(2565..3818)
                     /locus_tag="VM910_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66169.1"
                     /translation="MPKPRNARLESASARRKLAVRKKPYWTRISPGIALGYRRNEAAG
                     TWSVRVADGGAEWIKRIALADDLEPASPPAVLNYWKAIDQARALARRQPGEAIDESRP
                     LTVSEALTLYEKDLISRGGSPYNSQHAQLHLTASILSKPVGLLGATELTKWRDSLLAK
                     GLAPSTVNRTKTGLRAALELAAKHDPRIANQRAWKIGLCALPDAHVARNVILDDATVR
                     RIVAAAYDHDRALGVMVEVAAVTGARLSQLARLEVGDLQADRAEPRVFMPCSAKGRVR
                     TKRHERRAVPVTPALASVLKQEAAGRASDAPLLLRSNGERWGHGRSRHHRNDFRAVVE
                     AAGLDPDQVTLSAMRHSSIVRQLLANVPIRIVATLHDTSVKMIERTYSRYIASHSDEI
                     ARRALLDTSQQPTAANVVTLAERRP"
BASE COUNT          720 a         1310 c         1334 g          878 t
ORIGIN      
        1 aacgtacacg tcgtactgcg cgttgatctt cggtacgacc gcgtcgcgga cgtacgccca
       61 cgccttcttg tcgaggagat cagggatgat ctccatatcg agcggaacgc cgcggtgttc
      121 catctgcgcc gacaccgcga cgaattcgcc ccagtgcagc gcggtgccta gatcaaactc
      181 cggctccgcc atcagttgcg agaacaactc aaagagcgga tcgacatcgg tcatcacgta
      241 tttgaggatt tccgcttttt cctccggcgt gaaaggccat ccgcgcatga tgagcttgcg
      301 gattttttct ttgtagaccc cgtcgatcga agggatgccg tagtacgcga gtgcgccaag
      361 cagtcctttc ccctgcggca cgatgcggcc gttggcgacg cggcgaaaca gcggggagag
      421 atcgatgacc ttcgctggta attgccatcc cagagaaaga cggctcgcac cttcagcgtt
      481 aaagacgaaa gagaggaata gcgcctggtc gtcggtgcga ttgggggagg ttggacctag
      541 ttggtcgcgc cataatctca aggtccggcc ggagcgcagt tcacgggctc cgtaacagac
      601 gacgtcgtgc cgttcgccga gctttgggac ccattcgaag tcttcggccc aaatctcggc
      661 gaacggtaac gcaacaacgt tacttagatt tgtcacgagc cgcccacttg aggaacagtg
      721 ggtgatcggg gctgtcgacc aagcggccct tgtcgcgaaa cgctactctg aaaatcttgg
      781 catgcttgag ttcaggccaa atcggatcag agaaacgccc aaccggcgca gggaaaactt
      841 tgtagtttct gttttccctg tcgctataga gccgcaccca ctggcgagtc ccctcgatca
      901 gcccgatctc cttcgaacgg atgtactcat tgtcgctctc gacattgatg gggatggtcc
      961 gcacagcacc gcgcggggtc acgatcatgt agaggcggtg atcggagacc gtgatcccga
     1021 tccccgccaa cgcagccacc atgttgtcgg tcacggcaaa gaactgccgc tccatcccga
     1081 cctcgatgtt gaccatagga atgatcggca tgaagtcagg atgcgtccgg aagaactcgt
     1141 tctcctccgg cttcctgctg acggtgatcg acacgatgcc aatggcgcta gcgcctttga
     1201 caccagggaa atcacgacga agttttttga actcgatctc gtcttcgtcg aggtcttcgg
     1261 tgactacggc aagcttaggc tttgaagttt cgtctgccat gattggctcc tattttgatt
     1321 tgcacttttg tccgccactt tcggtcgcca gccgtcgtgc tgggtcgcgc gcttgatggt
     1381 gtcaggcacc ccttttcgat tcaggcggcg gcttcgcgcg ccttgcgcca ttcggcggcg
     1441 gcttcctgtg agatcagccg gcgtgagccg actgccatct cccgcgggcc gagcccttgc
     1501 gttttcagct tgtagtacat ggacaccgcg atgcggtgcg acaggcagaa ctcggcgatg
     1561 gtgtaggcgg cgggcggcgg tgtttgtctc ggcttctttc cggtgatctc tccgcgcgcc
     1621 atcgtcgtcg cctccatgat gaaagttcat ggaggacgct acgacgacgc ggagttttgt
     1681 cattgtgcga gttgcgcgcg cgcaaaacgc gcaaaacgcg caaaatgaga aaacagtctt
     1741 agcggcgatc tagcgcgcgt agaaacacgt cgcgtttggg gacggccaag cttctcgcct
     1801 caagcttgtc gctcaccttc ttgatgatct ctcgcgtttc gatgtgctcc cacccacccg
     1861 gccaaatctc ggcggctacc tctaggacta gctcctgctg gcgcgtgcgc ttgcgtttgg
     1921 ccgatgtttt gcgcggcgac ttgccgcttg tcgtcggcag cagcgcctcg atcatctcgt
     1981 cggcgtccat ctcccaggtg tacgcctcct caggcttgtc gagcgctcgc ttcgccgcga
     2041 tctcggcacg ccgtctccgc tgaccgtcgg cccgacgcga gccggggcct ttcacgacga
     2101 ggttggtccg gatgaagttc gggttgacta ggcggccatt ggaaaagagc cgcgcgcgct
     2161 tgtcgtcgcg cagcaatgcc ttgttcattt cgtcgacagc accgttgggg ccgagcgcgg
     2221 gccttagctt gcggaaggct ttgtcgattg agaccttctt tccgcgcctg ggcagcgctt
     2281 tcgccttcat cgcgcaggcc gccgcatgcg cccgtcgaac tgaaacgagc ccgtcgggcg
     2341 gccgcgctcg tcatagaacg tcgtcgtgtt gccggtcgtg gtcgacgtgg cgagcgaatg
     2401 tccgcgcgcg tcgtaatacc tcacgctggc gtcgccgagc ggaaccgccg tgccgatgct
     2461 ctggccattc ggctcataga agcgggtctg ctggcgatcc tgcgcctgcg ctgtcgccgt
     2521 cgcggcgagc acgagcgcaa aggctagaag tatctttttg atcttcatgg ccgccgctcc
     2581 gcgagcgtga cgacgttggc ggcggtaggc tgctgcgaag tgtcgagcag tgcccggcgc
     2641 gcgatctcgt cgctgtggct ggcgatgtaa cggctgtagg tcctctcgat catcttgacg
     2701 gatgtgtcgt gcagcgtcgc cacgatcctg atcggcacgt tggcgagcaa ttgccgcaca
     2761 atcgaagaat gccgcatcgc gctcaatgtc acttgatcgg gatcgagccc ggcggcctcg
     2821 accacggcgc ggaaatcgtt tcgatggtgg cggctgcggc cgtgtcccca tcgctcgccg
     2881 ttgctgcgca agagcaaagg ggcatcgctc gctcgcccgg cggcctcttg cttgagcacg
     2941 gatgcgagcg caggcgtgac cggcaccgcg cggcgctcgt gccgcttggt acgcacgcga
     3001 cccttggccg aacacggcat gaagacacgc ggctccgctc ggtccgcttg caggtcgccc
     3061 acttcgaggc gagccagttg actcagtcgc gcgccagtca ccgccgcgac ctcgaccatc
     3121 acgccgagcg cccgatcatg atcgtaggca gccgcgacga tgcggcgcac ggtggcgtca
     3181 tcgaggatga cgttgcgggc aacgtgcgcg tctggcagag cgcagagccc aattttccac
     3241 gcccgctgat ttgcgatgcg cggatcgtgc ttcgctgcca gttcgagcgc cgcgcgcagg
     3301 ccggtctttg tgcggttgac ggtgctaggc gcgagcccct tggccaagag actgtcgcgc
     3361 cacttcgtta actcagttgc gcccaacagc ccgaccggct tgctcaagat cgaggcggtc
     3421 agatgcagct gcgcgtgctg cgagttgtag ggactgccgc cgcgcgagat caaatctttc
     3481 tcgtagagcg tgagcgcttc gctcaccgtg agcgggcggc tctcgtcgat ggcctctccg
     3541 ggctgccgcc gcgcgagcgc ccgcgcctga tcgatggctt tccaatagtt caagacggcg
     3601 ggcgggctcg cgggttcgag atcgtcggcg agcgcgatcc tcttgatcca ctccgccccg
     3661 ccgtcggcga cgcggaccga ccatgtgccc gcggcttcgt tgcgccggta gcccaaggca
     3721 atgccggggc tgatcctcgt ccagtagggt tttttccgca ccgcgagctt gcggcgggcg
     3781 ctagcgcttt cgaggcgagc attgcgaggc ttgggcattg ggaatgcggc tccgtgtccg
     3841 gtcagtgtcc gaaatatagg agtggagcac catggagcgc aatagagcgt cgaagaggga
     3901 aatagcgatg ttgcaatatg ttacatgcaa cgggatggaa cggcatggaa cgtcatggag
     3961 cgaaaatttg ccatgacgcg ggacgctcgc ctctcggcgc tccaccgtgg cggttttagg
     4021 ctcccggggc cgcgcttgcc tcactggcat ttgcgccgga tcggttacag cgagctcctc
     4081 gcacccaggt cgtagtgccc ggagggcggg gtccctgcct cccggggcga acggttacaa
     4141 gccgccgccg caggacgcca cgcctcgctc cgccttcggg atcgtctccg gagacgcccc
     4201 tcgatgagcg aggctgacga agcttataat tcagcttcat ac
//
LOCUS       DATLJT010001164         5290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2381239,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001164 DATLJT010000000
VERSION     DATLJT010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381239"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM910_26830"
     CDS             <1..358
                     /locus_tag="VM910_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI66170.1"
                     /translation="MQALIGNILDRCITDAGYRGHNAPPDHKFKVYTSGQKRRITPQI
                     KREFKRRAAIEPVIGHLKEHHRMGRNYFAHASGDAINAVLAAAGYNFRRLLAWLRFLL
                     LRILIALGLAAQLKLA"
     gene            complement(499..669)
                     /locus_tag="VM910_26835"
     CDS             complement(499..669)
                     /locus_tag="VM910_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66171.1"
                     /translation="MQFDHMKTVKRPFQILKDGYAPGRKAGKRKVTLRSSPKKASELR
                     RENRDLHRSEFR"
     gene            complement(721..864)
                     /locus_tag="VM910_26840"
     CDS             complement(721..864)
                     /locus_tag="VM910_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66172.1"
                     /translation="MAAPHELAEMLVDPGNNLWCEDGKGTLYAYEVCDAVEAKHFRLT
                     VLL"
     gene            1233..4640
                     /locus_tag="VM910_26845"
     CDS             1233..4640
                     /locus_tag="VM910_26845"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66173.1"
                     /translation="MRCPKCQHENTAKAKFCEECAAPLPQTCAECGSRVSVTAKFCAQ
                     CGHPLEPVADDARFASPKSYTPQHLADKILTSRAAVEGERKQVTVLFADIKGSMELLA
                     SRDPEDAQKLLDPVLDRMIEAVHRYEGTVNRVMGDGIMALFGAPIAHEDHAVRACCAA
                     VRMQETVARYADEVQRSHGVPVAIRVGVNSGEIVVWTIGNDLHMDYTVVGQTAHLAAR
                     MEQMAKPGSVLTTADTFQLAEGYVPMKPLGPVPVKGLADPVPVYEVTGASAARTRLKA
                     AAGRGLTRFVGRGVELEQLRRAKQLAGQALGQVVAIIGEAGVGKSRLMHEFIHSDQTA
                     GWLVLETSSVSYGRATPYLPVIELLRHYFQINVHDSKQLVGEKVTAKILTLDAALQDA
                     IPPLLDLLDALDDEHPFRSLDLIQHRHHTYQAVVRLLLSETRVQPVIAFFEDLHWNDS
                     LTLGLLNELIIGAQKARLLLVVSYRLEYRDEWRGRPNYRQLRLDPLASETLAEFLQAL
                     LGSDPSLPPLKSFLAERASGNPFFAEEIVRALAGTHVLDGARGSYRLASPLSSVEIPP
                     TVQAVLAARIDALPAAERRLLHEAAVIGHDAPFSLLHAICGLTEDQLRRLLDHLQSAE
                     FLYVTQLFPDLQYTFKHALTHDVAYSGVLHERRRDIHARVVDAIEKLYADRLGEQVER
                     LAQHSVRAELQEKAVTYLRQAGGRAAARSALPDARAWFEQALSILKGLPESQATLEQA
                     FDIRLELRPVLRQLGEVRQMLEHLREAEALADRLRDDGRSGRVCAFMTTVLSTLNELD
                     QALVTGSRALEIAQRLGDLRLRIVSTGHLEQAHYYRGEYEHVIELATDNLAALPDDWA
                     HEYFGTAVPPSVFARGWLIMSLAEIGRFAEAAKYEAETIRLAEPTQHTHTIGWAYLAA
                     SVLHLLQGHWAQAHWLVEQGVNVRRSVDIAVLLPWAITSSAWALAQIGELSEASRRLR
                     EAEALLEGQTVRGIVGHRSWGYCATSRACLLLGRLEEARRLGARAFESSQRQPGFRAH
                     ALHLLGDLAVHPDRFDAESGEAHYREALALAQLHGMQPLAAHCHLGLGKLYHSTGKPE
                     HARENLAAAMTMYREMDMRFWLEQGKIDMTNSGDAEPPAMEILGPRH"
     gene            complement(4637..4960)
                     /locus_tag="VM910_26850"
     CDS             complement(4637..4960)
                     /locus_tag="VM910_26850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66174.1"
                     /translation="MSMRSAGPVPSSSADTTRGETKASGASSRTCRSTFPSRWAITAK
                     LAARPFAKSSFHWRALAIAIRRASRRVALIGTFLACCARAASGHATAAPLMNLMNPRR
                     LADVC"
     gene            complement(4986..>5290)
                     /locus_tag="VM910_26855"
     CDS             complement(4986..>5290)
                     /locus_tag="VM910_26855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66175.1"
                     /translation="LRDSPHVNDKVKLSEEEIQKLSPMRRPIVNKPLGIAYGTGELPA
                     MIVSSRDYHAYRSQGHVPGDLIPIANTNHFTILDELRQPNSTLTRAVIAMAQYQTA"
BASE COUNT          989 a         1657 c         1600 g         1044 t
ORIGIN      
        1 gatgcaagcg ctcatcggca acatcctcga tcgctgcatc accgatgccg gctatcgcgg
       61 ccacaacgcc cctcccgatc acaagttcaa ggtctacacg agcggccaga agcgccgcat
      121 cacgccgcag atcaagcgcg agttcaaacg gcgtgccgcc attgagcccg tcatcggcca
      181 tctcaaggaa catcaccgga tgggccgcaa ttacttcgcc cacgcaagcg gcgacgccat
      241 caacgccgtg ctcgccgccg ccggctataa cttccgccgc ctcctcgcgt ggttgaggtt
      301 tctgttgctc agaattctga tcgctctcgg cctagccgcc cagctcaaat tggcctgaaa
      361 ccgaattctt cacggacgac tccttaaggg cctcaagcca tcattcctcg gccggaacgg
      421 ctttacctaa aatcgagggc attcaaggaa gcaagtcacc tgagcccctc gcacgccggc
      481 catcagctca aatttccctc agcgaaattc gctgcgatgc agatcgcgat tttctctacg
      541 aagctcgctt gccttcttag gtgaactacg cagtgtgacc ttgcgcttgc ccgccttcct
      601 tccaggcgcg tagccgtcct tgaggatctg aaacggacgc ttcaccgtct tcatatgatc
      661 gaattgcact gagtttccgt ttgtggaatg cttcgaaaaa agctgagtag acgaagtcgc
      721 tcatagcaag accgttaacc ggaagtgctt ggcctcgacc gcgtcgcata cctcgtaagc
      781 gtaaagcgtg cccttgccgt cctcacacca aagattgttt cctggatcga ccagcatctc
      841 tgcgagctcg tgcggtgcgg ccaagctgac tttgtttcgg gcgctgagcg gcgaccttcc
      901 tgcaagtacg gttcttacga atacgagcgc aatcggacgg cctttgaaca tctggtaggc
      961 gaccacttcc ttggcgtgtt tgccaaagga tcttctttag gtcttcgcgg aggttcctga
     1021 tgtcatccgc catgttgaca aatatcatcg tctacgcgtt atctcgcggc ctagtcgtct
     1081 tcacaagttt cgccgacgta ctccagactg gagctaagtg ctagcctcca tgatgggctg
     1141 aggcattatg gcggaactta tgccttggcg ccgaccaccc gcaacgatac gctcgcagtg
     1201 tatctggctt gcttcttgct tcagggccgg caatgagatg ccccaagtgc cagcacgaga
     1261 ataccgccaa ggcaaagttc tgcgaggagt gcgccgctcc gctgccgcaa acctgtgcgg
     1321 agtgtggaag ccgagtatcg gttacagcca agttctgcgc ccaatgtggc catccgctgg
     1381 agcccgtcgc ggatgatgcc cgctttgctt cgcccaagag ttataccccg cagcaccttg
     1441 ccgataagat cctcacctcc agggcagctg tcgaaggcga gcgcaagcag gtcacggtgc
     1501 tgttcgctga tatcaagggc tcgatggagc tgcttgctag ccgcgacccc gaggacgcac
     1561 agaagctcct tgatccggtg cttgaccgca tgatcgaggc ggtccaccgc tacgaaggca
     1621 ccgtaaacag ggttatgggc gacgggatta tggcgctctt cggcgcgcca atcgcccacg
     1681 aagatcatgc cgtccgcgca tgttgtgccg cggtcaggat gcaagaaacc gtcgcgcgat
     1741 acgcggacga ggttcagcgc tctcacggcg tgccggtggc gatccgagtc ggggtcaatt
     1801 caggcgagat cgttgtctgg accatcggca atgatctgca catggactat acggtcgtgg
     1861 gccagactgc gcacttggcg gctcgcatgg aacagatggc aaagccgggt tccgtgctca
     1921 caacggccga caccttccaa ttggctgaag gctacgtccc catgaagccg ctcggccccg
     1981 tgccagtaaa ggggctcgcc gatccggtac ccgtctacga ggtgacggga gccagtgccg
     2041 cgcgaacccg cctgaaagcc gcggccggac gtggcttgac gcgtttcgtc ggccgtggcg
     2101 tggagcttga gcaattgcgc cgcgcgaagc agcttgccgg tcaggctctc ggtcaggtag
     2161 tcgcgataat tggcgaagcg ggggtgggca aatcacgcct gatgcacgag ttcattcatt
     2221 cagaccaaac cgccggctgg ctggttctgg aaaccagctc cgtttcttac ggtcgcgcga
     2281 cgccttacct gccggtcatc gagttgctta ggcattattt tcagatcaac gtgcatgaca
     2341 gcaagcaatt ggtcggcgag aaagtaacag ccaagatttt gacactcgac gccgcgttgc
     2401 aggacgcgat tcctccgctg ctcgatcttc tcgatgcgct ggatgacgag catccgttcc
     2461 ggtctctcga cttaatccag catcggcacc atacgtatca ggcagtcgtc cgcctgttgt
     2521 tgagcgagac ccgcgtacag ccggttatcg cgttcttcga ggaccttcac tggaacgatt
     2581 ctcttacact cggcttgctg aatgagctaa tcatcggcgc gcagaaggcg cgcttgctcc
     2641 tcgtggtcag ttatcgccta gagtacagag acgaatggag gggccgaccg aattatcgcc
     2701 agctacgtct ggatccactt gccagcgaga cccttgcgga attccttcag gccctgttgg
     2761 gctccgaccc gagtctcccc cctctcaaga gcttcctggc ggagcgcgcc agcggcaatc
     2821 cgttcttcgc agaagagatc gtgagagcgc tggccggtac tcatgtgctc gacggcgcgc
     2881 gcggcagcta ccgccttgcg agtccgctct cgagcgtcga gatcccgcct acagtgcagg
     2941 cggtgctcgc cgcgcgtatc gacgcgctac ccgccgccga gaggcgactg ctgcatgagg
     3001 cagcggtcat tggacatgat gccccgttca gcctattgca cgcaatttgt gggctgacgg
     3061 aggaccagct ccgccgcttg ctcgaccacc tgcagagcgc cgaatttctc tacgtgacac
     3121 agctctttcc tgacctgcaa tacaccttca agcatgccct tacccacgat gtggcgtaca
     3181 gcggagtgct ccacgaacgc cgccgcgaca tccacgcccg ggtcgtggat gccatcgaaa
     3241 agctctacgc cgaccggcta ggtgagcagg tggagcgtct cgcccagcac tcggttaggg
     3301 ccgagctgca ggagaaggct gtgacctatc ttcgccaagc cggcgggagg gcggccgcgc
     3361 gatcggccct cccggatgcg cgggcctggt tcgagcaggc cctgagtatt ctcaaaggtc
     3421 ttcccgagag tcaggccacg ctggagcaag ccttcgacat tcgcctcgag ctgcggccgg
     3481 tgctgcgcca gctcggtgaa gtgcggcaga tgcttgagca cctgcgcgag gcggaggcgc
     3541 ttgctgatcg attgagagac gatggtcgga gtggccgggt ctgcgctttc atgacgacgg
     3601 tcctgtcaac gctcaacgag ctggaccaag cgctcgtgac tggtagccgc gccttggaga
     3661 ttgcccagcg tctcggcgat ctgaggcttc gcatcgtcag cacgggccat ctcgagcagg
     3721 cgcactacta ccgcggcgag tacgagcacg tgatcgagct cgccaccgac aatcttgcgg
     3781 cgctgccgga cgactgggcg cacgagtatt tcgggacggc cgtgccacca tccgtctttg
     3841 cccgcggttg gctcatcatg agtctcgccg aaatcggcag attcgccgag gcggccaagt
     3901 acgaggcgga aacgatccga ctcgcagagc caacgcagca cacacacaca atcggttggg
     3961 cgtacttggc cgcaagcgtg cttcatctcc tccagggcca ttgggcgcag gcgcactggc
     4021 tggtagaaca aggggtaaat gtacgtcgga gcgtagacat cgccgtcctt cttccttggg
     4081 cgatcacctc atccgcctgg gcgctggcgc agatcggcga gcttagcgag gcctcgcgcc
     4141 ggctgcgaga agccgaggcg ctcctcgagg gccaaacggt acgggggatc gtcggtcatc
     4201 gcagctgggg gtactgcgcg acgagtcgcg cttgtctgct acttggccgg cttgaggagg
     4261 cgcggcggct gggcgcccgc gcgtttgaat cctcgcagcg tcagcccggc tttagagccc
     4321 atgcgcttca tcttctcggt gacttggcag tccatcccga ccggttcgat gccgaaagcg
     4381 gcgaggctca ctaccgggag gcgctggcac tcgcccagtt gcacggcatg cagccactcg
     4441 ccgcccactg ccatctcggc ctaggcaagc tctaccactc caccggtaag ccggagcacg
     4501 cgcgcgagaa tcttgctgcc gcgatgacga tgtatcgcga gatggacatg cgcttctggc
     4561 tcgagcaggg gaagattgat atgacgaact ccggcgatgc ggagccaccc gccatggaaa
     4621 tcttaggtcc caggcattag cagacgtccg caagtctgcg ggggttcatg aggttcatca
     4681 gcggcgcggc agttgcgtga ccgcttgcgg cgcgcgcgca gcaggcaagg aaggtgccga
     4741 tcaaagctac ccgccgcgag gccctcctga tcgcgatcgc caaggctcgc caatggaacg
     4801 acgacttggc gaacggccgc gcggccagtt tcgccgtgat cgcccagcgg gaaggaaagg
     4861 tcgagcggca cgtccggctg ctggcgccgc tcgccttcgt ctcaccccgg gtcgtgtcgg
     4921 cactgcttga gggcaccggg ccggcggatc tcatactcac caagctcgcc cgcgcgccga
     4981 atcccctacg cggtctggta ttgcgccatc gcaatcacgg cgcgggtgag cgtgctgttg
     5041 ggttgtcgca gctcatccag aatcgtgaag tgattggtgt tggcgatcgg tatcaagtcg
     5101 cctgggacat gcccttgcga ccggtaggca tggtaatcgc ggctgctcac gatcatggcc
     5161 ggcaactcgc cggtgccgta ggcgataccc agcggcttgt tgacgatcgg ccgccgcata
     5221 ggcgagagct tctggatttc ctcctcgctc agcttcacct tgtcgttgac atgcggggag
     5281 tcgcgcagcg
//
LOCUS       DATLJT010001165         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1233081,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001165 DATLJT010000000
VERSION     DATLJT010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233081"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..1955)
                     /locus_tag="VM910_26860"
     CDS             complement(480..1955)
                     /locus_tag="VM910_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HVI66176.1"
                     /translation="MTLHFPFQNTYAALPANFFARVNPTPVASPRLIKLNRPLAVHLG
                     LDPDLLESPEGAEILSGKTLPAGADPIAMAYAGHQFGHFVPQLGDGRAILLGEVIDKD
                     GVRRDIQLKGSGPTPFSRRGDGRAALGPVLREYVVSEAMYALGIPTTRSLAAVITGES
                     VIRETALPGAVLTRVAASHIRVGTFQYFAAREDTDAVRRLADHVIARHYPELSGANRP
                     YHALLSAVTTRQAELVARWLLIGFIHGVMNTDNTSISGETIDYGPCAFLDHYDPAQVF
                     SSIDEFGRYAYANQPRIALWNLTRFAECLLPLFSGEQEKAIAEAQEALGGFAEAFNTA
                     YKAGLRKKLGLATERDDDQALAQALLDTMAKGRADFTLTFRRLSDAAGEETNDRVRQL
                     FAEPEAFDEWATRWRQRLAQEPQSPVERQAAMRAVNPAFIPRNHRVEAVIAAAVTNDD
                     YAPFEELLTVLSKPYEDQPALADYANPPEPHERVCQTFCGT"
     gene            2111..>2364
                     /locus_tag="VM910_26865"
     CDS             2111..>2364
                     /locus_tag="VM910_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVI66177.1"
                     /translation="MHRYRTHTCGALRESHIGEIVRLSGWCHRIRDHGGVLFIDLRDH
                     YGITQCVVDPDSAAFADAENLRSEWVVRMEGKVRHRPEGT"
BASE COUNT          422 a          719 c          789 g          434 t
ORIGIN      
        1 cgatcttgct ttgcttgtac gcgccgattg cttcactttc gggtagggaa gcggattcgt
       61 ctgcgatgcc gccttggcgc agggcaatgg tggcaacact gctggtatga acgatccatg
      121 actctgccca cagccgttcc gccgaaaggg ctgatatgtc aagggtctcg gcgtgaattc
      181 ggagcttgca tttggccgag accgaaagac gtcagccagc cgccgactca acgtcggcgt
      241 cgcggtgcgc cggggggctt tgcgatcagt aacggcgagg gcgccgggac ggattttggc
      301 ttgagggaac tgcgatcacg acgacgcaga gcgccagcac cgtcagtccc atgaactcga
      361 acacgagagc gtccacgaca acatccccca acaaaccggt agctttgtcg ggggcgcgtt
      421 gaccgttcct taatcgcggg cgccggcggt cgtcgcatgg tggacggggc gttaatgcat
      481 caggttccgc agaacgtctg gcacacgcgc tcgtgcggct ccggagggtt cgcgtaatcg
      541 gcaagggccg gctgatcctc gtagggcttc gaaagaaccg tgagcagctc ctcgaaggga
      601 gcgtagtcgt cgtttgtgac agcggccgca atcacagcct cgacgcgatg attgcgcgga
      661 atgaaggccg gattgaccgc gcgcatcgcg gcctgccgct cgaccggcga ttgcggctcc
      721 tgagcgaggc gctgtcgcca gcgcgtagcc cattcgtcaa acgcctccgg ttcggcaaaa
      781 agctggcgca ccctgtcatt cgtctcttct cccgcagcat cgctcaggcg gcggaaggtg
      841 agggtgaaat cggcacggcc cttcgccatc gtgtccaaca gcgcctgcgc caaggcctgg
      901 tcgtcgtcgc gttcggtggc gaggccaagc tttttccgca gccccgcctt gtaggcggtg
      961 ttgaaggctt ccgcgaaccc gccgagcgcc tcctgcgctt ctgcgatcgc tttctcttgt
     1021 tccccggaga acagcggcag caggcactcg gcaaagcgcg tcaaattcca cagtgcgatt
     1081 cttggctggt tggcataggc atagcggccg aactcatcga tcgagctgaa gacttgcgcg
     1141 gggtcgtagt gatccaggaa tgcgcagggt ccgtaatcga tggtctcgcc cgaaatcgat
     1201 gtattgtccg tgttcatcac gccgtggatg aaaccgatca ggagccagcg ggcgacaagc
     1261 tcggcctggc gcgtcgtcac ggccgacagc agcgcatgat aggggcgatt tgcgccggac
     1321 aattcgggat agtgcctggc aatgacatgg tcggcgagcc ggcgaactgc gtccgtatcc
     1381 tctcgtgcgg caaagtactg gaatgtgccg acgcggatgt ggctcgctgc gacgcgggtc
     1441 aggaccgcgc cggggagtgc cgtctcgcgg atgacgcttt cgccggtgat gacggcggca
     1501 agggagcggg tggtcggaat ccccaatgcg tacatcgcct cgctcacgac gtactcacgc
     1561 aacaccggtc cgagcgcggc gcggccatcg ccgcggcggg aaaacggcgt cggacccgag
     1621 cccttgagct ggatatcccg gcgtacgccg tccttgtcga tgacctcgcc gagcaggatg
     1681 gcgcggccat ctcccagttg gggaacaaaa tgcccgaact ggtggcctgc ataggccatg
     1741 gcgatcggat cggcaccggc gggcagcgtc ttgccggaaa ggatctccgc gccttccggg
     1801 ctctcgagca ggtccggatc gaggccaaga tggaccgcga gcggccggtt cagcttgatc
     1861 aaccgggggg aggcgaccgg cgtcgggttg acgcgggcga aaaaattcgc cggcagcgcc
     1921 gcgtaggtgt tttggaaggg gaaatgcagg gtcatgcacc caagataggc gtgatcaggc
     1981 gttttttcga gcccgaaacc cgcggcgctt cggttgccgc gccacgtcgc ctcgggtaaa
     2041 ccctcccgcg cgtttcggac cggccatttg gtccccgatt cgaacctccg acatcgactt
     2101 cgagatattc atgcatcgtt accggaccca tacctgcggc gcgctccgcg agagccatat
     2161 cggcgaaatt gttcgcctgt cgggctggtg ccatcgcatc cgcgaccatg gcggggtcct
     2221 gttcatcgac ctgcgcgacc attacgggat cacccaatgt gtggtcgatc cggactcggc
     2281 ggcatttgcg gacgccgaga atctgcgctc ggagtgggtg gtgcggatgg agggcaaggt
     2341 acgccaccgc cccgaaggca ccga
//
LOCUS       DATLJT010001166         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2613185,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001166 DATLJT010000000
VERSION     DATLJT010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613185"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VM910_26870"
     CDS             complement(<1..429)
                     /locus_tag="VM910_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI66178.1"
                     /translation="MLDVQDQRLLAQLQKDSRQSNQQLADRVGMSASACWRRVDSLEK
                     AGVITRYTVLVDRAQAGFAMSAIIHVSLDRHDEKFVTQFVSRVKQRPEVLECFATTGD
                     ADYHLRVVVSDMTAYNRFLDEFMFKLPGIRHVRTNVILKEI"
     gene            588..4061
                     /locus_tag="VM910_26875"
     CDS             588..4061
                     /locus_tag="VM910_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVI66179.1"
                     /translation="METATAGNLDAYALLDRYVRQSGRVFLTGTQALVRILLDQRDRD
                     RADGLKTAGFVSGYRGSPLGGVDLELWRAKQFLAERRIEFLPAVNEDLAATAVLGSQQ
                     VETNPKREVEGVFGLWYGKGPGVDRSGDALKHGNAYGSSPHGGVLVVAGDDHGCVSSS
                     MPHQSDVAFMAWFMPTLNPSNVAEYLSFGEYGYALSRFSGMWVGFKAISETVESCASV
                     ELRPRRKFNIPAFDMPPGGLYYRWPDLPGPQIEQRLEFKKNAVLAFAAANPIDRRIYD
                     IPDATYGIVTTGKSHLDVMEALELLGLTEAECRRLGIDIYKVGMVWPLAHHDALEFVR
                     EKSEILVIEEKRGIIESQFKEYFYDYPGHKPERMVGKTDENNYPLVPWIGELSPRLLA
                     PIIAQRLDAMFPGLRLGERAARLQPSPGRIVNVEGATRTPYFCSGCPHNTSTKVPAGS
                     QALAGIGCHFMASWMDRDTTSLIQMGGEGVNWVASSLFTGRPHVFQNLGEGTYYHSGS
                     MAIRQAVAAGANITYKILFNDAVAMTGGQPVDGPISVQRIAHELRANGVARIALVSDD
                     PSKFDAHDFPDDVTIDPRTRLDAVQRALREVPGVSALIYEQECATERRRKRKRGQADD
                     PKKFAYINELVCEGCGDCSIESNCLSVEPKETPFGRKRRINLSTCNKDFSCLNGFCPS
                     FVTIEGGMRRKPRGSNQVDVVSLAARLPVPSRAPLERPFNLLVTGVGGTGVITIGTLV
                     AMAAHLQGNGASVLDFTGFAQKFGPVLSFVRISERPETIHQVRIDHGSADALIGCDIV
                     VSSSSRASTTFGPSMRAVLNMAEMPTGDVVRNRDAGLATNERRQSIERVIGADNLMSF
                     DANRAASVLFGDTVFANMIMLGAAWQRGLVPVSCEALMRAIELNGVAIEDNKRAFASG
                     RLIAADTSFAASLEEKDSKKEALDDKIAKRVAFLTDYQNASYAARYLAKINEVRAAES
                     TAHPGSEVFTTAVAISLFKLMAYKDEYEVARLHLDSAFRDKLRQEFEGDFTIRYHLAP
                     PLLPSGRDARGRPLKRQFGAWIEPAFRLLTRLRVLRGTPFDFFGYTRERKMERSLITW
                     YEALIDALLPLLTTRPIDRLGAIASLPLDIRGYGPVKDASIEAAKAKFLAMMQAVLSQ
                     SSGEGPHQPGR"
     gene            complement(4022..>4352)
                     /locus_tag="VM910_26880"
     CDS             complement(4022..>4352)
                     /locus_tag="VM910_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HVI66180.1"
                     /translation="FWGVMDNKRWVYPYDAKGERRPFKDLPKSVKDLKDDPFRSLAGE
                     LRRAGGFAKETTPFSEFLWADFLRRKMQRKLAEDQFAKALEKALMLAKSKEAVYLPGW
                     CGPSPDD"
BASE COUNT          853 a         1344 c         1287 g          868 t
ORIGIN      
        1 gatttccttg aggatgacat tcgtcctgac atgacggatg cccggaagct tgaacatgaa
       61 ctcgtcaagg aagcggttat aggcggtcat gtcgctgacg accacgcgta gatgataatc
      121 cgcatcgcca gtggtcgcga aacattcgag cacttcgggc cgttgcttca cgcgcgagac
      181 aaattgcgtg acgaactttt cgtcgtggcg gtccagcgac acatgaatga tggcggacat
      241 cgcaaatcca gcctgcgcgc ggtcgacaag caccgtatag cgggtgatca cgcccgcctt
      301 ttccagcgaa tccacccggc gccagcaggc tgacgccgac atgccaacgc gatcggcaag
      361 ctgctgattg ctctgccggc tatccttctg cagttgtgcg agcaggcgct gatcctggac
      421 gtccagcatt ttcgacgctc caaatctggt cgatttatcc gtttttctgc caattaagga
      481 taatactacc acaattgcct tcgacctcgg atgaaattga aaaacctcgc cacccgatca
      541 ggccgatagt ttgaatcaga catgcatctc ccgaatggag ggcggccatg gaaacggcga
      601 cggcgggcaa cctcgacgct tacgcactgc tcgatcgtta tgtcaggcaa agcgggcgcg
      661 tttttctgac cggcacgcag gccttggtgc gcatcctgct cgaccagcgc gaccgcgatc
      721 gcgccgacgg cctcaaaacc gctggcttcg tttcgggcta tcgcggctcg ccacttggcg
      781 gcgtggacct tgaactgtgg cgcgccaagc aatttcttgc agagcgtcgg atcgaattcc
      841 ttcccgcagt gaacgaggat cttgccgcca ccgcagtgct cggctcgcag caggtcgaga
      901 ccaatccaaa gcgcgaggtc gaaggcgtat tcggcctctg gtatggcaag ggacccggcg
      961 tcgaccgctc cggcgacgcg ctgaagcatg gtaacgctta tggatcgtcg ccgcatggcg
     1021 gcgtcctcgt cgtcgcaggc gacgatcacg gctgcgtgtc gtcctcgatg ccgcatcagt
     1081 cggacgttgc gttcatggcg tggttcatgc ccacgctcaa cccgtcaaac gtggccgagt
     1141 atctgagctt cggcgaatac ggttatgcgc tgagccgctt ttccgggatg tgggtcggct
     1201 tcaaggcgat ttcggaaaca gtggagtcct gcgcttccgt tgagctgcgc ccacgccgca
     1261 agttcaacat tcccgccttc gacatgccgc ccggcgggtt gtactatcgc tggccggatc
     1321 ttcccggtcc gcagatcgag cagcggctgg agttcaagaa gaacgcagtg ctggcttttg
     1381 ccgcggcaaa cccgatcgac cgcagaatct acgacatccc cgacgcgacc tatggcatcg
     1441 tcacgaccgg caagagccat ctcgacgtga tggaggcgct cgagctgctc ggcctcaccg
     1501 aagcggaatg ccgccgcctc gggatagaca tttacaaggt cggcatggtg tggccgctcg
     1561 cccatcatga cgcgctggaa tttgttcgcg aaaaaagcga gatccttgtg atcgaggaga
     1621 agcgcggcat catcgagagc cagttcaagg agtatttcta cgactatccc ggccacaagc
     1681 cggagcgcat ggtcggcaag accgacgaga acaattatcc gctggtgccc tggatcgggg
     1741 agctgtcgcc gcgcctcttg gcgccgatca tcgcgcagcg gttggatgcg atgtttcccg
     1801 gccttcgcct tggcgaacgt gcggctcgcc tgcagccatc accaggtcga atcgtcaatg
     1861 tcgaaggggc cacccgaacg ccttatttct gctccggctg tccgcacaac acctcgacaa
     1921 aggtaccggc ggggtcgcag gcgctagctg gaatcggctg tcactttatg gcgagttgga
     1981 tggatcgcga cacgacctcg ctgatccaga tgggcggcga aggcgtgaac tgggttgcat
     2041 cctcgctgtt caccggccgc ccgcatgttt tccagaatct cggtgaaggc acctattatc
     2101 attcggggtc gatggcgatc cgccaggcag tcgccgctgg cgccaacatc acctacaaga
     2161 tcctgttcaa cgacgcggtt gccatgaccg gcggccagcc ggtggacggt ccgatcagcg
     2221 tccagaggat cgcccatgag ctgcgtgcca acggcgttgc gcgcattgcc ctggtctcgg
     2281 atgatccttc gaaattcgac gcgcacgatt ttcccgacga cgtcacgatc gatcctcgca
     2341 cgcgcctcga tgccgtccaa cgagcgttgc gtgaagtgcc gggcgtctcc gcactgatct
     2401 acgagcagga atgcgccacc gagcgacgcc gcaagcgcaa gcgcggccag gcggacgatc
     2461 ccaaaaagtt cgcctatatc aacgaactcg tctgcgaggg ctgtggcgat tgctcgatcg
     2521 aatcgaactg cctcagcgtc gagcccaaag aaacgccgtt cggccgcaag cgccggatca
     2581 acctgtcgac ctgcaacaag gatttctcct gcctcaacgg cttttgccca agcttcgtga
     2641 cgatcgaggg cgggatgcgc cgcaagccgc gcggatcgaa ccaagtcgat gtcgtttcac
     2701 ttgccgctcg gcttcccgtg ccgtcacgcg cacccctgga aagaccattc aacctgctgg
     2761 tgaccggcgt cggcggcacc ggcgtcatca ccatcggtac actggtggcg atggccgcgc
     2821 atcttcaagg caatggcgcg tccgtgctgg actttaccgg ctttgcccag aagttcggac
     2881 cggtcttgag cttcgtcagg atttcggaac gacccgaaac cattcatcag gtccgcatcg
     2941 atcacggttc ggctgacgca ttgatcggct gcgacattgt ggtcagttcc tcgtcgaggg
     3001 cgtcgaccac cttcggtcca tcgatgcggg ccgttctcaa catggcggaa atgccgactg
     3061 gcgacgtggt ccgcaatcgc gacgccgggc ttgcaacgaa tgaacgacgg caaagcatcg
     3121 agcgggtcat cggggcggac aatctgatga gcttcgatgc caatcgcgcg gcaagcgtgc
     3181 tgttcggcga caccgtcttt gccaacatga tcatgttggg tgctgcctgg cagcgaggac
     3241 tcgtgccggt ttcgtgcgag gcgctgatgc gcgcgatcga actcaacggc gtcgctattg
     3301 aggacaacaa gcgggcgttc gcgagcggcc ggctgatcgc ggcggatacg agcttcgcag
     3361 catctcttga agagaaggac agcaagaagg aagcgctgga tgacaagatc gccaagcgtg
     3421 tcgcattcct gacggactat cagaatgcgt cctatgccgc ccgctatctc gcgaagatca
     3481 atgaggtgcg cgcggcggaa agcaccgcgc acccgggctc ggaggttttc accacagccg
     3541 tcgccatctc cttgttcaag ctcatggcct ataaggacga gtatgaggtg gcacgacttc
     3601 atctcgattc ggcctttcgc gacaagctgc gccaggagtt cgagggcgac ttcaccattc
     3661 gatatcatct ggcaccgccc cttctcccct cagggcgaga tgcgcgcggc cgaccgttga
     3721 agcggcaatt tggtgcctgg atcgaaccgg cttttcgact gctgacacga ctccgcgtcc
     3781 tgcgcggcac gcctttcgat ttcttcggct acacgcgcga gcgaaaaatg gagcgcagtc
     3841 ttattacctg gtatgaggcg ctgattgatg cactcttacc gctgctgacc acgaggccca
     3901 tcgatcgcct tggcgcgatc gcgtccctgc ccttggacat ccggggatat ggccccgtca
     3961 aggacgcctc gatcgaagct gcaaaggcaa aattccttgc gatgatgcaa gcggtcttgt
     4021 ctcaatcgtc cggcgaggga ccgcaccagc ccggcagata gaccgcctcc ttgctcttgg
     4081 cgagcatcag cgccttctcg agcgcctttg cgaattgatc ctccgcaagc ttgcgctgca
     4141 tcttgcggcg cagaaaatcc gcccacaaga attcgctgaa cggcgtggtt tccttggcga
     4201 agccgccagc gcgccgcaac tcaccggcca ggctgcggaa cggatcgtct ttgagatcct
     4261 tcaccgattt cggaagatcc ttgaaaggcc gccgttcgcc cttggcatca taaggataga
     4321 cccagcgctt gttgtccata actccccaga ac
//
LOCUS       DATLJT010001167         6340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_771706,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001167 DATLJT010000000
VERSION     DATLJT010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771706"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VM910_26885"
     CDS             complement(<1..660)
                     /locus_tag="VM910_26885"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HVI66181.1"
                     /translation="MDYAKPTDVIASMIDAGQKKLALGPRDLMIRGALSGALLGAATT
                     LAFTGAVTTGQPLVGALIFPVSLVMIVLLGLELVTGSFALVPLPRLEGTTTWSAVVAN
                     WSWVFIANLLGSVAFGVLIAISLTNMGKVEVAGVAARIVSVAEAKTIGNAALGTAGMV
                     SVFVKGVLCNWLVCLGVVMAMTSTSTVGKIAATWLPIFLFFALGFEHAVVNMFIIPTG
                     ML"
     gene            complement(992..2155)
                     /locus_tag="VM910_26890"
     CDS             complement(992..2155)
                     /locus_tag="VM910_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI66182.1"
                     /translation="MTTPLHVGFIPLIDAAALIVAVDKGFAAELGLDVTLVREVSWSN
                     VRDKLNIGLFDAAHLLAPVAIASSLGLGHVKVPIVAPFNLGVNGNAITVSPALHAALM
                     AEIEGDAFDPMATARALARVVARRRKSGAEPLTFGMTFPFSTHNYQLRFWMAAGGVDP
                     DEDVHLVVLPPPYMVDSVANGHVDAFCVGAPWNSIAVDRGVGHILHFVSDIFVHAAEK
                     VLAVRQGWSEKNPQVVAALVRAASRAAEFIEHPANRAEVARILAQPERIGVDAEVVRR
                     TLDGRLKVSPDGSIRESGRYLLVGREGAARPDPVQAAWLYAQMARWRQTSLSPEALKT
                     AIAVFRPDLYDAALGKTAPAVPNRPIGPFAGPAFDPNDIAGYLASFEIPRRTR"
     gene            complement(2152..2742)
                     /locus_tag="VM910_26895"
     CDS             complement(2152..2742)
                     /locus_tag="VM910_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVI66183.1"
                     /translation="MSAEQSPKIVIVDESPIRAAILEEGLREAGYTSVVRISEMQSLL
                     ARIYALDPDIIVIDLENPSRDILEQMFQVSRAVRRPIAMFVDQSDAASIQASVDAGVS
                     AYIVDGLKKERIKPILDLCVSRFNAFARLQDELDRTKSALEERKVIDRAKGILMKLKG
                     LTEEEAYVLMRSTAMREKKKIGEIAQSILTASELLK"
     gene            2855..4180
                     /gene="rimO"
                     /locus_tag="VM910_26900"
     CDS             2855..4180
                     /gene="rimO"
                     /locus_tag="VM910_26900"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HVI66184.1"
                     /translation="MQQAAAPKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGA
                     DLVIVNTCGFLDSAKQESLSAIGEAMAENGKVIVTGCMGAEPEQIEQAYPGVLSITGP
                     QQYESVLDAVHRALPPAHNPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCIIPKL
                     RGDLVSRSADDVLREAERLVKAGVKELLVISQDTSAYGVDLKYAASQWKDREVRAKFV
                     DLARELGSLGIWVRLQYVYPYPHVDDVIPLMSEGKILPYLDIPFQHASPEILRAMRRP
                     AAQDKTLARIARWREQCPELAVRSTFIVGFPGETDADFAYLLDWLEEAEIDRVGCFKY
                     EPVAGAASNALPGAVPDDVKRHRYDALMARQQKISTRRLKRKIGTRQQVIIDEVGPTV
                     ARGRSKADAPEIDGSVYISSRRPLKVGEIVTARIERADEYDLHGSVVGF"
     gene            4194..4424
                     /locus_tag="VM910_26905"
     CDS             4194..4424
                     /locus_tag="VM910_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66185.1"
                     /translation="MPRPLKVKIFMDTNASAVEEQINAWLVSQASASIIKTETMVGGA
                     TEKSSGAQPYIVVTIWYEPPASDQERPGFRIA"
     gene            4717..5913
                     /locus_tag="VM910_26910"
     CDS             4717..5913
                     /locus_tag="VM910_26910"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HVI66186.1"
                     /translation="MTNATKPFDALMDITARPRTVFVRGEGSYLWDDGGKRYLDFIQG
                     WAVNCLGHSPPALARALADQAKNLITPSPAYYNDKALVLARALVDKSCFDQVFFANSG
                     AEANEGAIKLARKYGSLHRGGAYEIITFEGAFHGRTLATMSASGKKAFEPLFEPKVSG
                     FRKARLNDLSSVEELIGANTVGVMLEPIQGEAGVWPASDQFLKSLRALTEQHGLLLIF
                     DEIQTGMGRTGKLFHYEHAEIVPDIMTLGKGIGGGAPLAALLATEKASCFEHGDQGGT
                     FNGNALMCAAGLAVLEEVSKPEFLKAAADAGLFLESELQKLSARHGLGEVRGRGLLLA
                     LDLKLPIAPAIVAQAFETGLLLNAPQADTLRFMPALNVARAEMLEMIDGLDAILTREG
                     AARRVA"
     gene            complement(6013..>6340)
                     /locus_tag="VM910_26915"
     CDS             complement(6013..>6340)
                     /locus_tag="VM910_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVI66187.1"
                     /translation="GKSTFPGSLSCLKPRGLFVSFGNASGPVPPFAISELNSHGSLFA
                     TRPKLNDYIGTRKELLEGADTLFAAVISGKLHVPINHAYALKDAAKAHLDLEARTTTG
                     ASILKP"
BASE COUNT         1249 a         1947 c         2000 g         1144 t
ORIGIN      
        1 cagcatgccg gtcgggatga tgaacatgtt gaccacggcg tgctcgaaac cgagcgcgaa
       61 gaacagaaag atcgggagcc acgttgccgc gatcttgccc acggtcgagg tcgaggtcat
      121 cgccatcacg acgcccaggc acaccagcca gttgcagaga acgcccttga cgaagacgct
      181 aaccattccg gccgtgccaa gcgcggcgtt gccgatggtc ttggcttctg cgaccgaaac
      241 gatgcgcgcc gcgacgccgg cgacctcgac cttgcccata ttggtgagcg agatggcgat
      301 caggaccccg aacgcgacgc taccgagcag gttggcgatg aagacccagg accaattggc
      361 gacaacggca ctccatgtcg tcgtgccctc gagccgcgga agcggcacca gcgcaaagct
      421 tccggtgacg agttcgagtc cgagcagcac gatcatcaca aggctgaccg gaaagatcag
      481 cgcgccgacc aaaggctgtc cggtggttac ggcgccggtg aacgccagtg tggtggcggc
      541 gccgagcagc gcgccggaaa gagcaccgcg gatcatgagg tcgcgcgggc cgagggccag
      601 cttcttttgg ccggcatcga tcatcgaggc aatgacgtcg gtgggcttgg cgtaatccat
      661 ttgaggctcc tgccacgcct ggcgtggccg caacgaggaa taggggacgc gtgatcaccc
      721 gtgagttttg cgagcgatcc gatcagcttc gttgctgcgg atatccatcc tggattgacc
      781 ggcggccatc agcggcggct ggcgtgtaat tgcttattca aaacatgtgc caacttggcg
      841 cggtgcaaaa agcaaaacac tttcaatatg atgttcgttt tgcggtgcat taagtggata
      901 accgttcatg ccttttcaaa gagcaaaagc tcaaactcta tgcagcgaat cagggtgctt
      961 aaattttgac caggagaaga tccggaggga ctcaacgcgt ccggcgcggg atctcaaagg
     1021 aagcaaggta gcccgcgatg tcattcggat cgaaggctgg tccggcgaat gggcctatcg
     1081 gacggttggg gaccgccgga gcggtcttgc caagggccgc atcgtaaaga tcgggccgaa
     1141 acaccgctat ggccgtcttg agcgcctccg ggctgagaga ggtttgtctc cagcgtgcca
     1201 tctgcgcgta gagccaggcc gcctgcaccg gatcggggcg cgcggctcct tcccgtccga
     1261 cgaggagata gcggccgctc tcacggatgc tgccgtcggg cgagaccttg aggcgcccgt
     1321 ccagagttcg ccgaacaact tcggcgtcga cgccgatcct ttcgggttgg gccaggatgc
     1381 gcgccacttc ggcgcgattg gcaggatgct cgatgaactc ggcggcgcgt gaggcggcgc
     1441 gcacgagggc cgcaacaacc tgcggattct tctccgacca gccttgcctg acggccagca
     1501 ccttttcggc cgcgtgcacg aagatgtcgg aaacgaaatg caggatgtgg ccgacgccgc
     1561 gatcgaccgc gatggaattc cagggggcgc cgacacagaa ggcatcgaca tgtccgttgg
     1621 ccacactgtc gaccatgtag ggcggcggca acacgaccag gtgcacatct tcgtccgggt
     1681 cgacgccacc agccgccatc cagaaccgca attgataatt gtgagtggaa aacggaaagg
     1741 tcatgccgaa ggtcaagggc tcggcgccgg atttgcgccg cctggcgacc acccgcgcca
     1801 gcgcccgcgc ggttgccatc ggatcgaagg cgtcgccttc gatttcagcc atcagcgcgg
     1861 catgcagtgc gggcgacacg gtaatggcat tgccgttgac gccgagatta aagggggcaa
     1921 cgatcgggac cttgacgtgg ccgagcccga ggctcgaagc gatcgccacc ggcgcgagca
     1981 gatgcgccgc gtcgaacagg ccgatgttga gcttgtcccg cacattcgac cacgagacct
     2041 cccgcacgag cgtcacatcg agccccaact cggcggcaaa tcccttgtcg accgccacga
     2101 tcagggccgc ggcatcaatc aacgggatga agccgacatg cagcggtgtg gtcatttcag
     2161 aagctccgac gcggtcagga tcgattgggc gatctccccg atcttcttct tttcgcgcat
     2221 tgcggtggag cgcatcagga cataggcctc ctcttcggtc aggcccttca atttcatcag
     2281 gattcccttg gcgcgatcga tcaccttgcg ttcttcgagg gcggatttgg tgcggtcgag
     2341 ctcgtcctgc agcctggcga aggcattaaa gcgcgacacg cagaggtcga ggatcggctt
     2401 gatgcgctcc ttcttcagcc cgtcgacgat gtaggcggac acgccggcat cgacggacgc
     2461 ctggatcgag gccgcgtcgc tctggtcgac gaacatcgcg atcggccgcc gcacggcccg
     2521 gctgacctgg aacatctgtt cgaggatgtc gcggcttgga ttctccagat cgatcacgat
     2581 gatgtcgggg tcgagggcgt agatgcgggc gagcaggctc tgcatctcgc tgatccggac
     2641 gacgctggta tagcccgcct cccgtaatcc ctcttccagg atcgcggcac ggatcgggct
     2701 ttcgtccaca atcacgattt tgggcgactg ctccgcgctc atcgatcact catagtccgg
     2761 catactatcc atagcacgcc cggcgccggc tcaaagcctt gtaattgtgc gcaattggga
     2821 ctagctcacg accatcaatc gggcagtttt tgatatgcaa caggccgccg cgcccaaagt
     2881 cagctttgtt tcgctcggat gtcccaaggc tttggtggat tccgaacgca tcatcacgcg
     2941 tctgcgcgcg gaaggttacg agcttgcccg caagcatgac ggcgccgacc tcgtcatcgt
     3001 caatacctgc ggcttccttg acagcgccaa gcaggaatcg ctttccgcga tcggcgaggc
     3061 gatggcggag aacggcaagg ttatcgtgac cggctgcatg ggcgcggaac ccgaacagat
     3121 cgagcaggcc taccccggcg tgctgtcgat caccgggccc cagcaatatg agagcgtgct
     3181 ggacgccgtg catcgcgcgc tgccgcccgc acataatccg catctcgacc tggtgccgcc
     3241 gcaaggcatc aagctgacgc cgcggcacta cgcctacttg aagatctccg aaggctgcaa
     3301 caacaggtgc agcttctgca tcattccgaa gctgcgcggc gatctggtgt cgcgcagcgc
     3361 cgacgacgtg ttgcgcgagg ccgagcgact ggtcaaagcc ggcgtcaagg agctcctcgt
     3421 catttcgcag gacacctcag cctatggcgt cgatctcaaa tacgccgcca gccaatggaa
     3481 ggaccgcgag gtccgcgcaa agttcgtgga tctcgcgcgc gagctcggct cgctcggcat
     3541 ctgggtgaga ttgcaatacg tctatcccta tccgcatgtc gatgatgtca ttcccctgat
     3601 gagcgagggg aagatcctgc cctatctcga tatccccttt cagcacgcaa gtccggagat
     3661 cctgcgagcg atgcgacgcc cggccgcgca ggacaagacg ctcgcgcgga tcgccagatg
     3721 gcgcgagcag tgccccgagc ttgccgtgcg ctcgaccttc atcgtcggct tccccggcga
     3781 gaccgatgcg gatttcgcgt acctgctcga ctggctggaa gaggccgaaa tcgaccgcgt
     3841 cggctgcttc aaatacgaac cggtcgcagg cgcggcctcg aatgcgcttc ccggcgccgt
     3901 tcctgacgat gtcaagcggc atcgctacga tgcgttgatg gcacggcagc agaaaatctc
     3961 gacgcggcgg ctcaagcgca agatcggcac gcgccaacag gtgattatcg acgaagtcgg
     4021 tccaacggtc gccaggggcc gttccaaggc ggatgctccg gagatcgatg gctcggttta
     4081 catttccagc cggcgtccac tgaaggtcgg cgagatcgtc accgccagga tcgagcgcgc
     4141 agacgaatac gatctgcacg gcagcgtcgt gggattctaa aggggcaaac aacatgccaa
     4201 ggccgttgaa ggtcaagata ttcatggaca ccaacgcaag cgcggtggaa gagcagatca
     4261 atgcctggct ggtttctcag gcttcggcga gcattatcaa aaccgaaaca atggttggag
     4321 gcgcgaccga gaaatcgagc ggcgcccaac cctatattgt cgtcaccatc tggtacgagc
     4381 cgcccgcctc ggaccaggag cgacccggtt ttcgtatcgc ttgaccctcg agatcggcga
     4441 caatggactc gcactctatc ggggcgatga gcgaatgctg cgcgcccttg acagcgaagc
     4501 cccttccgct gtatgcccac gccccatgat tttgaaccgg accaaagccc gcgcccgttg
     4561 ccgtcgtcac atccgtgacg atgaagggtc gcgccatgtc cgacgacaac gctgaaatca
     4621 gctaatcagc agagtttcga gaaggccgct acccccaagt agcggccttc tccgttttcg
     4681 gccctcgctt ttcaccaaac cttcaaggag cctgccatga ccaacgccac aaaaccgttc
     4741 gatgcgctga tggacatcac cgcgcgcccg cggacggtct tcgtgcgcgg cgaaggttcg
     4801 tatctctggg acgatggcgg aaagcgctat ctcgatttca tccagggctg ggccgtgaac
     4861 tgcctcggcc attcgccgcc ggcgctggcg cgggctctcg cggatcaggc caagaacctc
     4921 atcacgccga gcccggccta ctacaacgac aaggctttgg tgctggcgag ggcgctggtc
     4981 gataaaagct gctttgacca ggtgtttttt gccaattccg gcgcggaagc caatgaaggc
     5041 gcgatcaagc tcgcgcgcaa atatggcagc ctgcaccgcg gcggcgccta tgagatcatc
     5101 accttcgaag gggcttttca cggccgcacc ctcgcgacaa tgtcggcatc cggaaagaag
     5161 gcgttcgagc cgctgttcga gcccaaggtg tccggcttcc gcaaagcgcg cctgaacgat
     5221 ctctcttccg tcgaggagct gatcggtgcc aacaccgtcg gcgtgatgct ggagccgatc
     5281 cagggcgaag ccggcgtgtg gccggcgagt gatcagtttc tcaagagttt gcgggcgctg
     5341 acggaacagc acggactgtt gctcatcttc gacgagatcc agaccggcat gggccgcacc
     5401 ggaaaactct tccactatga gcacgccgag atcgtgcccg acatcatgac gctgggcaaa
     5461 ggaatcggcg gcggcgcgcc gcttgcagcc ctgcttgcga cggaaaaagc ctcctgcttc
     5521 gagcatggcg accagggcgg caccttcaac ggcaacgcgt tgatgtgcgc ggccggcctt
     5581 gcggtgctcg aagaggtgag caagccggaa tttctaaagg cggctgccga tgccgggctg
     5641 ttcctggaga gcgaattgca aaagctctcg gcgcgtcacg ggcttggcga ggtgcgcggc
     5701 cgtggcctcc tgctcgccct tgatctgaaa ttgccgatcg cgcctgccat cgtggcacag
     5761 gcgttcgaga ccggtctcct cctcaatgcg ccgcaggcgg acacgctgcg cttcatgccg
     5821 gcgctcaacg tagcgcgggc cgagatgctt gagatgatcg acggtctcga tgcgatcctg
     5881 acgagggagg gcgcagcgcg gcgcgtggct tgatgccatg gagaagcaaa ccatgcttct
     5941 gcgccgccgg cgcatgccgc aaatgctgcg agcgttcacc ccccgcggcg ggcgccgtat
     6001 cggaagcgcg attcagggct tgaggatcga agcgccggtt gtggtgcggg cttccagatc
     6061 gagatgggct tttgcggcat ccttcagcgc ataggcgtga ttgatcggca cgtgcagctt
     6121 gccggagatc acggcggcaa acagcgtgtc ggcgccttcc aggagttcct tgcgcgtgcc
     6181 gatatagtcg ttgagctttg gccgcgtcgc aaacagcgag ccgtggctgt tgagctcgga
     6241 gatggcaaac ggcggcaccg gtcctgacgc gttgccgaag ctgacgaaca ggccgcgcgg
     6301 cttcaggcag gacagcgaac ccgggaatgt cgatttgccg
//
LOCUS       DATLJT010001168         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4219622,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001168 DATLJT010000000
VERSION     DATLJT010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4219622"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /locus_tag="VM910_26920"
     CDS             complement(<1..2344)
                     /locus_tag="VM910_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI66188.1"
                     /translation="MTTSRTEKYRPLWLAALAMVALPLALKALGLSLNTGIMVVALSI
                     AALGLNLCIGYTGLVSFGHSTWFGIGAYAAGLIQLNWFRDQIWLPLALSMVVVAIVST
                     IVGIIILRRRGVYFSLLTLALAALSYTIAFRWSAVTGGEDGLGGLKRGTIGPFNLDNA
                     LTYYVTVAVIALAVLYVLLRLARSPFGHVLVAIRENQLRATFQGYPVQRYKLAVFVIS
                     AMVTGLAGALIGFQNYLVSADAVSVPFAGELLAMVVIGGMRSMLGPALGALFFILFRE
                     LFSIWTANWLLLFGLTFAGFVMFSPEGLVGIWNQLMRRLKPPPEEAAAMSKRKIYNDL
                     PPPAFLKPQAFEGVVLEVNDVSKHFGGIRAVRDASLQISVGEIHALIGPNGAGKTTLF
                     NLISGLFPSDAGTIKLRGREIQNVPSDLICHQGLARSFQITNLFKGLSIYENLRLSLQ
                     ARSPGRFNLWRDTDDFADVHAETAELVKFLGLEGIEGIAGGDLSYGGQRLVDLGIALG
                     SKPQVLLLDEPLAGLAAAERERVSNLVQNIAANIPVLIVEHDIDRVLGLSKTVTVMNQ
                     GEVLMTGTPGAVRSDPLVQEIYTGKGIPAIAHPCAGRAKADTAPLLRFERVNTFYGKS
                     HILNDASLDVRDGEIVALLGRNGAGKTTLLKTLAGLVPAASGTIQYEGRDIAQLPAPD
                     IARLGVGYVPQGRGLFAGMTVRENLALGRLARKTDGSHGIVWDEERIFAHFPRLKERM
                     EVAADYLSGGEQQMVAVARAMSGNVKLLLLDEPFEGLAPAV"
     gene            complement(2341..>2782)
                     /locus_tag="VM910_26925"
     CDS             complement(2341..>2782)
                     /locus_tag="VM910_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66189.1"
                     /translation="LAVVAAVLLGVWLLLHKTSFGRVVRAGIQRPDMVASLGIRLEPY
                     MTAVVMLGVGMAALGGAFFAPITIVHPAMGAEIITVAFVVVVIGGLGSFWGVVIAALL
                     VGVVRGITIHFVPAAGEASIYILMFLVLLVRPRGLLGERIEKFE"
BASE COUNT          572 a          885 c          892 g          433 t
ORIGIN      
        1 tcaccgccgg cgcaaggccc tcgaacggtt cgtccagcag caggagtttc acattacccg
       61 acatcgcgcg cgcgaccgcc accatttgct gctcgccgcc ggagagataa tccgccgcga
      121 cctccatgcg ttccttcaag cgcgggaagt gcgcaaagat ccgttcctcg tcccacacga
      181 tgccgtggct gccgtcggtc ttgcgggcga ggcgcccgag cgcgaggttc tcgcgcaccg
      241 tcattccggc gaacaggccg cgcccctgcg gtacataacc gaccccgagg cgcgcgatat
      301 cgggtgcggg aagctgcgca atatcgcgac cttcatattg aattgtgccc gatgcggccg
      361 gcacaaggcc cgccagcgtc ttcaacagcg tggtcttgcc ggcgccgttg cgcccgagca
      421 gcgcgacgat ctcaccgtcg cggacatcca ggctggcgtc gttgaggatg tggctcttgc
      481 cgtaaaacgt gttgacgcgc tcgaagcgca gaaggggcgc ggtgtcggcc ttcgccctgc
      541 cggcgcaggg atgggcaatg gcgggaatgc ctttgcccgt ataaatctcc tgcacgagag
      601 gatcgctgcg aacggctcca ggtgtgccgg tcatcaggac ctcgccctga ttcatcacag
      661 tgacggtttt ggaaagcccc agcacgcgat cgatgtcgtg ctcgacaatc aggaccggaa
      721 tgttcgccgc gatgttctga accaggttcg agacgcgttc gcgctcggct gcggcaagtc
      781 cggcgagcgg ctcatccagc agcagcacct gcggtttgga gccgagcgcg atgccgagat
      841 cgaccaggcg ctgcccgcca taggagagat cgccgccggc aatcccctcg atgccctcga
      901 gccccagaaa cttcacgagc tccgccgtct cggcatgaac atcggcgaaa tcatcggtat
      961 cgcgccagag gttgaaacgg ccggggctgc gcgcctgcag cgacaggcgc agattttcgt
     1021 agatcgacag tcccttgaac aaattggtga tctggaacga gcgggccagt ccctgatggc
     1081 agatcaggtc ggacggcaca ttctggattt cgcggccccg cagcttgatg gtgccggcat
     1141 ccgagggaaa caggccggag atcaggttga acagcgtggt cttgccggcg ccgttcggac
     1201 cgatcagcgc gtggatttcg ccgacgctga tttgcaggct tgcgtcgcgc actgcgcgga
     1261 taccgccgaa atgttttgaa acgtcgttga cctccagcac cacgccttca aacgcctgcg
     1321 gtttcaggaa ggccggcggc ggcagatcgt tgtagatctt gcgcttgctc atggcggcgg
     1381 cttcctccgg cggaggtttc aggcgccgca tcagttggtt ccagatgccg accaggcctt
     1441 ccggtgaaaa catcacaaag ccggcaaagg tgagtccgaa cagcaagagc cagttcgcgg
     1501 tccagatcga aaacaattcg cggaacagga tgaagaacag cgcgccaagc gcggggccca
     1561 gcatgctgcg catgccgccg atcacgacca tggcgagcag ttcgcctgca aacggcaccg
     1621 acacggcatc cgccgagacc aggtagttct ggaagccgat cagcgcgccg gcgagccccg
     1681 tcaccatcgc cgagatcacg aacaccgcca gcttgtagcg ctgaaccgga tagccctgaa
     1741 aggtggcgcg cagttggttc tcgcggatcg cgaccagcac atggccgaag ggtgagcggg
     1801 ccagcctcag cagcacatag agtacggcga gcgcaatgac cgcgaccgta acgtaatagg
     1861 tgagcgcgtt gtcgaggttg aacggcccga tcgtgccgcg cttcaaaccg ccaaggccat
     1921 cttcgcctcc ggtcaccgcg ctccagcgga aagcgatggt gtagctcagc gcggcgagcg
     1981 cgagcgtcag cagtgagaaa tagacgccgc gtcgccgcag aatgatgatg ccgacgatcg
     2041 tcgagacgat cgccacaacg accatcgaca aggcgagcgg cagccagatc tggtcgcgaa
     2101 accagttgag ctggatcagg cccgccgcat aggcgccgat gccgaaccag gtcgaatggc
     2161 cgaacgagac cagcccggta tagccgatgc agagattgag ccccaaggcc gcgatcgaaa
     2221 gtgcgaccac catgatgccg gtgttgagtg acagcccgag cgccttgagt gccagcggca
     2281 aggcgaccat cgccagcgct gcgagccaga gcggccggta tttttcggtg cgcgaggtcg
     2341 tcattcgaat ttctcgatcc gctcgccgag cagcccgcgg ggccgcacca gcagcaccag
     2401 gaacatcagg atgtagatcg atgcttcgcc ggccgcaggc acgaagtgaa tggtgatgcc
     2461 gcgcacgacg ccgaccaaaa gcgccgcgat gacgacgccc cagaagctgc cgaggccgcc
     2521 gatcacgacg acgacaaagg caactgtaat gatctcggcg cccatcgccg gatgcacgat
     2581 agtgatcggc gcaaagaacg cgccgccgag cgcagccatg ccgacgccga gcatgacgac
     2641 tgccgtcata taaggctcca ggcggatgcc gagcgaggcg accatgtcgg gtcgttgaat
     2701 gccggcgcgc accacgcggc cgaacgaagt cttgtgcaag agcagccaga cgccgagcag
     2761 caccgcggcg accacggcga gg
//
LOCUS       DATLJT010001169         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2842769,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001169 DATLJT010000000
VERSION     DATLJT010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842769"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1300
                     /locus_tag="VM910_26930"
     CDS             266..1300
                     /locus_tag="VM910_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI66190.1"
                     /translation="MAAAFARELAQLAAGQPSNSMLREPRWTTPNRVALELPAMRLRE
                     FSADGDGIATLVCAPLALHDATLTDFAPDHSLVAALQMAGLRNLFVTDWRSASPDMRF
                     FSIDSYLADLNVVVDELGGCANLIGMCQGGWMALVYAARYPGKVRGLVLAGAPVDTNA
                     GESELSRLAHSAPASMAKQLVAVGDGCVRGAHLLQLWRHPPLEPQAIHDLLQVADDMP
                     GTSALIARFREWYMRPIDLPGTYYLEVVQRLYKDNQLATGRFAALGRPIDLAMVRGPI
                     FLLAARHDEVVAPEQLLAARHLVGSKELRTEVAACTHLGLFMGATTLRQTWPEIAHWL
                     AATSAGLLTS"
     gene            1822..2127
                     /locus_tag="VM910_26935"
     CDS             1822..2127
                     /locus_tag="VM910_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66191.1"
                     /translation="MVIASNKTSIMLGLLVCALGISVSSSADARKANRQGTYVEQGYP
                     NPTPGITVMPRNTPRANIVCWTPCGQPGAIVMGADPDPNVRAYLLRDASGFFGGGSR"
BASE COUNT          449 a          754 c          768 g          488 t
ORIGIN      
        1 gcggtgccgg cggtgcagga gcgattggcg acgttcggcg tcgagccgat gccgatgacg
       61 gtggacgagt tcggcaaatt ctaccgcgac gacgtggcgg cgatcgtcaa gctggcgaaa
      121 gacatcaacc tggtgccgac caactgatac gccgcgcgtg cccggctgcg ttggaccgtg
      181 tgatgatcgt accgaccgac ggtgcgccgt ggttcctttg acgccctttc tgtggccggc
      241 cctcgccgcg gcgacggcga gcgacatggc cgcggcgttc gccagggaac ttgcgcagct
      301 ggccgcgggg cagccctcga actccatgtt gcgcgagccg cggtggacga cgccgaaccg
      361 cgttgcgctc gaattacccg cgatgcggct gcgcgagttc tcggcggacg gcgatggcat
      421 tgccaccttg gtttgtgcgc cgcttgccct ccatgacgcc acgctgacgg atttcgcgcc
      481 ggaccacagt ctcgtcgcgg cactgcaaat ggccgggctg cggaatctat tcgtgacgga
      541 ttggcgctcg gcgagcccgg acatgcgctt cttctcgatc gacagctacc tcgccgatct
      601 caacgtcgtg gtggacgagc tcggcggctg cgccaatctc atcgggatgt gccaaggcgg
      661 ctggatggcg ctggtctatg ccgcgcgcta tcccggcaag gtgcgcgggc ttgttctggc
      721 cggggcaccg gttgacacta atgccggcga atccgaacta tctcggctcg cccacagcgc
      781 gccggcgtcc atggcgaaac aactggtagc ggtcggcgat ggatgcgtcc gcggcgcgca
      841 tctgctgcag ctctggaggc atccgccgct cgaaccgcaa gcaattcatg acctgctgca
      901 ggttgcggat gatatgcccg gcacctctgc actgatcgcg cgctttcgcg aatggtacat
      961 gcgtcccatc gatctccccg gcacctatta tctcgaggtc gtgcaacggc tctataaaga
     1021 caaccagctt gccacgggcc gtttcgcggc gctcggacgc ccgatcgatc tcgcgatggt
     1081 gcgaggcccg attttcctgc tcgcggcacg ccacgacgag gtcgtcgctc cggaacagtt
     1141 gctcgccgcc agacatcttg ttggcagcaa ggaactccgc acggaggtcg cggcttgcac
     1201 gcatctcggg ctgttcatgg gggcaaccac gctgcgccag acctggcccg agatcgcgca
     1261 ctggctcgcg gctacttctg ccggcctgct gacgagctga tcgagtgaaa cggggtgaat
     1321 tttttcgacg cggcgcgctt gacgcgcggc gatccgcgaa cagagccaag tctcttcgtc
     1381 gcgaacggta gcagaggtcg cggggtcaaa tgctcaatgc gagcagttca atcgcgccga
     1441 tcccgattac cagcacagcg agggctaccg gatcgtagtc cagccaatac cgctttgttg
     1501 gcatggtacg cctccctgtt cccaccctat tagtcggacc ggcaggcaaa ttagttcaaa
     1561 tgggccgcgc aaattttcac aacgcattgg agctgcttat gaatcgaccg cacccccggc
     1621 caacccgtag ggccagcttg cagccccgca tgatataatg gggccttaga cccggacggg
     1681 aaacaccccg acgggaaacc acgtgagacg gatcgcttcc ttttgatttc ccggctctgg
     1741 ggcactgctc tagcgttcgc ccaggagccg ccataggcgc ccgagtctcc cggctccgat
     1801 aggaatcgat ggagaaattc catggtcatt gccagcaaca agacgtcaat catgcttgga
     1861 ctgcttgttt gcgctctcgg catttcagtc tcttcctctg ccgatgctcg gaaggcgaac
     1921 agacaaggca cgtatgtcga gcagggatac ccgaacccga cgccgggcat aacggtcatg
     1981 ccgcgcaata cgccgagggc taatatcgtc tgttggactc cttgcggtca gcccggcgct
     2041 attgttatgg gcgctgatcc cgatccaaat gtccgggcat atttgcttcg cgatgccagc
     2101 ggcttcttcg gcggtggctc gcgataaaag gagcgctcgc cgatcgagtt aggggttagg
     2161 gtccgcagcg tttcggttag gcgctgccga gaggggtaat ggcggccgac gatcgcctca
     2221 cgctgcattg cacgctttcc gcaattttcc gttctcgttg gcgagagctt cacgggccag
     2281 ctgaattgtt gtcacgtcgg gcagtcgaaa gatggtcccc gaccattggt tacgaggatt
     2341 taatcagaac gccaatgtcc gttggatgaa gcaagcatta ggctgatcaa ggtcaaagtc
     2401 agcaggtcat tcaaggatag cgagaaggcg ccatgaaccg acgacatttc ggattgagc
//
LOCUS       DATLJT010001170         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1923655,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001170 DATLJT010000000
VERSION     DATLJT010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923655"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VM910_26940"
     CDS             <1..573
                     /locus_tag="VM910_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVI66192.1"
                     /translation="RSLLDLCRSRRLTLATAESCTGGLVAGALTDIPGSSDVVDRGFV
                     TYSNEAKRAMLGVNATTLATFGAVSKETATQMAIGALERAGVDLAISTTGIAGPGGAT
                     PGKPVGLVHFAAAARDGRIIHREHRFGAIGRSAVRERSVVEALRMLIELARSPQAAKK
                     PVREAPSRLRPRAVRTPRRHAVKRRRPPRG"
     gene            714..929
                     /locus_tag="VM910_26945"
     CDS             714..929
                     /locus_tag="VM910_26945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66193.1"
                     /translation="MKLGLLALIAICSVSASLPASAMPIAPLASSTAPTDLVQIGHGG
                     HGHHYGWHHGHGHHYGWYHGRHHGWRH"
     gene            complement(978..1244)
                     /locus_tag="VM910_26950"
     CDS             complement(978..1244)
                     /locus_tag="VM910_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVI66194.1"
                     /translation="MSTAKLRACTILAEFDQAQAELVGKAVVLTDGKAGTVDRLWLDE
                     FHGLRISIVGHEGKWPVSTIKFAEHELYAGEPGTSHAFRRRSFH"
     gene            complement(1411..1878)
                     /locus_tag="VM910_26955"
     CDS             complement(1411..1878)
                     /locus_tag="VM910_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI66195.1"
                     /translation="MPRFSSKHRVQHAAAQMFDLVADVERYPEFVPLCKSLKTRQRKV
                     NADGTETVLADMTVSFKLVKETFTTRVTLDRANLKILVEYLRGPFSSLENRWTFEPKS
                     DTACGVGFFIAYEFKSRVLATLMGSMFDAAFARFSAAFEKRADKIYGKRPAVS"
     gene            complement(1891..>2163)
                     /locus_tag="VM910_26960"
     CDS             complement(1891..>2163)
                     /locus_tag="VM910_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVI66196.1"
                     /translation="LGEERHEVLQVMDDLRSADVDFLTIGQYLQPTRKHHAVIRYVPP
                     DEFAGLERVAFTKGFLMVSASPLTRSSHHAGKDFARLKAARAAIVR"
BASE COUNT          396 a          704 c          673 g          390 t
ORIGIN      
        1 cgctcgctgc tcgatctctg ccgcagtcga aggctgacgc tcgcgaccgc cgaatcctgc
       61 accggcggtc tcgttgccgg tgcgctcacc gacatccccg gctcctccga tgtcgtcgac
      121 cgcggcttcg tcacctattc caacgaagcc aagcgcgcga tgctcggcgt caatgcaacg
      181 acgctcgcga cgtttggcgc tgtcagcaaa gagaccgcga cgcagatggc gatcggcgcg
      241 ctggaacgcg ccggtgtcga tcttgcgatc tcgaccacgg gcatcgccgg acccggcggc
      301 gcgactccgg gcaagcccgt cggtctcgtg cattttgctg cggccgcgcg cgacgggcgg
      361 atcattcacc gcgagcatcg gtttggtgcg atcgggcgct ccgcggtgcg cgagcgctcg
      421 gtagtggaag cgctgcgcat gctcatcgaa ctcgcgcgca gtccgcaggc cgcaaaaaag
      481 ccggtccgcg aggccccaag tcgcctgcgt ccgcgcgccg tgcgcacacc gcgccgtcat
      541 gcggtgaaac ggagacggcc gccgcggggg tgaggacgca agggcggtct cgatgtgcca
      601 cagacgcgag cccgtgcaga tgccggcgaa caaattgcgt tccagtcggg gaactcgatc
      661 agggcgagcg gcttattgtg ccgcgcgcct gtggcgctcc ctgaggagga ctcatgaagc
      721 tcggtttgct cgctctgatc gcaatttgct ccgtatccgc cagcctgccc gcatctgcaa
      781 tgccgatcgc acctctggcg tcatcgactg caccaaccga cctcgtgcag atcgggcatg
      841 gcggtcacgg ccatcattat ggctggcacc acggccacgg tcatcattac ggttggtatc
      901 acggccgtca tcacggttgg cggcactagc gcggtgacgc cggacgtcgc ctccgtccag
      961 cgcatgcagc aaagtggtca gtggaacgat cgtcgccgaa atgcgtgaga agttcccggt
     1021 tcgccggcgt atagctcgtg ttccgcgaac ttgatcgtcg agaccggcca cttgccttcg
     1081 tggccgacga tcgaaatgcg caggccgtgg aactcgtcaa gccagagccg atctaccgtg
     1141 cccgccttgc cgtccgtgag cacgacggcc tttccaacca gctcggcctg cgcctggtcg
     1201 aactctgcaa ggatcgtgca ggctctgagc tttgcggtcg acatggctac tcatcggaga
     1261 tgggggctgg tgcgcaaacg cgttgcgaca cgtacacgac atctcgaaaa atcttgtcgg
     1321 gggcgaggag ataataaggc aattctctgc gggcggtgtg caccaaaaaa tagatccctg
     1381 cgctcgcgac gctgcccggg acgacagcgc ctacgacacc gccggccgct tgccgtagat
     1441 cttgtcggcg cgcttctcga acgctgccga aaagcgcgca aaggccgcat cgaacatcga
     1501 gcccatcagt gtcgcgagca cccggctttt gaactcgtag gcgatgaaaa agccgacgcc
     1561 gcaagcggtg tcgctcttgg gctcgaatgt ccagcggttt tccagactgg agaaaggtcc
     1621 gcggagatat tcgaccagga tcttcaagtt cgcccggtcc agcgtcaccc tggtggtgaa
     1681 cgtctccttc accagcttga acgacaccgt catgtcggcc agcaccgtct cggtgccatc
     1741 cgcgttgacc ttgcgctgac gggtcttcaa cgacttgcac agcggcacga attcgggata
     1801 gcgctccaca tccgcgacaa gatcgaacat ctgtgccgcc gcatgttgga cccggtgctt
     1861 gctggaaaag cgtggcatcg caagagcgcc ttagcggacg atcgccgccc gtgctgcctt
     1921 cagcctggca aaatccttgc cggcatgatg cgacgaacgg gtcaaggggc tcgccgacac
     1981 catcaagaat cccttggtaa aggcaaccct ttcgagcccg gcaaactcgt ccggagggac
     2041 atagcggatc accgcatggt gcttgcgggt cggctgcaga tattgcccga tggtcaggaa
     2101 gtcgacgtcc gccgaacgca gatcgtccat cacctgcagc acctcgtggc gctcttcgcc
     2161 gag
//
LOCUS       DATLJT010001171         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1694250,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001171 DATLJT010000000
VERSION     DATLJT010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694250"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3145)
                     /locus_tag="VM910_26965"
     CDS             complement(<1..>3145)
                     /locus_tag="VM910_26965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI66197.1"
                     /translation="RLCQADHSFIFLRQGDGYRCASGSGDIPEWIEYLKQQTIRPGRG
                     TIAARAVLERRTVHIPDVLADLEYTFLEAQRRGGYRTALGVPLLREGDPVGVIVLTRP
                     TVRPFDANQIELVTTFAAQAVIAIENTRLLNELRESLDRQTATADILRVIASTPEDST
                     RALDTIAETAVRMFNAASVSLRRVEGNVLRVIGAAGPSAAKVREILPDFPLESPDPAA
                     RSVFDNRQIHIEGRRAVLPNEGGEIARVLPALPLGSQAFTPLSRQGEAIGVMVVTRSE
                     VRPFQQIELDLMRGFADQAVIAIENARLLSELRQSLEQQTATSEVLTIISRSPAGLEP
                     VFQAILANATRLCGAKFGTLNLYDGEVVHRSASYNVPPEYAEMRLRMGRWRPHPGSAT
                     AQVVRTKELIHFDDLRTSPAYLEGDSSTRAIVDTGGARTLLMVPMLKDDQLIGTIAIY
                     RQEVRSFTDKQTELVENFAAQAVIAIENARLLNELRESLEQKTATSEVLSVISSSPDN
                     IQPVLEIIGERAEKLCDADVSVTSIVDGDLIRLSSVHGVTEAGVKAVRHAFPMRLTDE
                     TVTARAIRTRSVCHVADVLSDLKYPPKDAVHAAQVGGYHGCLGVPMVRDEQVVGAIFV
                     AKKQPGLFSDAQVQLLKTFADQAVIAIENVRLFNETKNSLEQQTATADVLKIISRSTF
                     DLQTVLQTLVESAARFCAADNATVVREKDGLFYTAEAYGYSREFLNYLRNIPIKAERG
                     SASGRALVEGRVVHIPDVKADPEYTFVEAPRLGDFRTALCVPMLREGIPIGVLTLTRS
                     EVQPFTDKQIELVTTFSDQAAIAIGNVRLFDEIQDKSRQLEVASQHKSQFLANMSHEL
                     RTPLNAILGYTELMADGAYGEPSEKMLGILKRLEANGRHLLGLINDVLDLSKIEAGQL
                     VLELSDYSVQDIAQTVRSTLEPLAADKKLAFRVEMAPELPPGRGDGRRLTQVLINLVG
                     NAIKFTDAGEVAIKAEANNGSFHVSVRDTGPGISAADQTRLFQEFQQADNAITKKKGG
                     TGLGLAISKRIIEMHGGR"
BASE COUNT          653 a          898 c          905 g          689 t
ORIGIN      
        1 ccgaccgcca tgcatctcga tgatgcgctt cgatatggcc agccccaagc cagtgccgcc
       61 cttcttcttg gtgatggcat tgtcggcctg ctggaattcc tgaaacagcc tcgtttgatc
      121 ggcagcggag atcccgggtc cggtgtcgcg aacggataca tggaatgagc cattattagc
      181 ttcagcctta atcgcgactt cgcccgcatc ggtgaactta atggcgttgc caacgagatt
      241 gatcaggacc tgcgtcaagc gccggccgtc gccacgaccg ggtggcagtt caggagccat
      301 ctcgaccctg aatgcgagct tcttgtcggc cgccagcggc tcaagcgtgc tgcggaccgt
      361 ctgggcgatg tcctgaacag agtaatcgga gagttcgagg accagttggc cggcctcgat
      421 cttggacaga tcgagcacgt cattgatgag tccgagcagg tgccggccgt tggcctcaag
      481 acgctttagt atgccaagca ttttctcgga tggctcgcca taagcgccgt ccgccatcaa
      541 ttcggtataa cccaagatgg cattgagcgg cgtgcgcagt tcgtggctca tattggccag
      601 gaattgcgat ttgtgctggc ttgccacttc aagctggcgg ctcttatcct ggatttcatc
      661 gaacagccgc acattcccaa tggcgatcgc ggcctgatcg gaaaaggtcg tgaccagttc
      721 gatctgcttg tcggtgaatg gctgtacttc cgagcgcgtc aaggtgagca cgccgatcgg
      781 gatgccctcg cgcagcatgg ggacgcagag agccgtgcgg aaatcgccca atctcggcgc
      841 ttcaacgaat gtgtattccg ggtcagcctt tacgtcgggg atatgaacga cgcgaccttc
      901 gaccagcgcg cgccccgacg ccgatccccg ctcggccttg attggaatat tcctaagata
      961 gtttagaaat tcgcgggaat agccgtaggc ctcggctgtg tagaagagcc catctttctc
     1021 gcggacaacg gtggcgttgt cggcagcgca gaaccgggca gctgattcaa cgagagtttg
     1081 caacactgtt tgcagatcga aggtcgagcg gctgatgatc ttgagaacgt cggcggtagc
     1141 agtctgctgc tccaaggaat tcttagtttc gttgaacaac cgcacatttt cgatcgcaat
     1201 caccgcctga tcggcgaagg ttttgaggag ttgaacctgt gcgtctgaga ataatcccgg
     1261 ctgtttcttc gcaacaaaga tcgctccgac aacctgctca tcgcggacca tcggcacacc
     1321 caagcaaccg tgataaccgc ccacctgcgc ggcatgcaca gcgtcttttg gtggatattt
     1381 aagatcgctc agcacatccg cgacatgaca aacgctacga gtgcggatcg cgcgcgccgt
     1441 gactgtctcg tcagtgagcc gcattggaaa tgcatgccga acagctttca ccccagcctc
     1501 tgtcacgcca tgaaccgacg acagacggat taggtcgccg tcgacgatgg atgtgacgct
     1561 cacgtcggca tcgcaaagtt tctctgctcg ttcgccaatg atttccagca caggctgaat
     1621 attatctggc gaactcgaaa tgacacttag cacctccgac gttgcggtct tctgctcgag
     1681 agattcacgc aattcgttga gcagccgcgc gttctcgatg gctatgacgg cttgggcggc
     1741 gaaattctcc accaactcag tctgtttgtc ggtaaaggac cgaacttctt ggcgatagat
     1801 cgcaatagtg ccgatcagtt gatcgtcttt gagcattggc accatgagaa gggtgcgagc
     1861 gccgcccgtg tcaacgatgg ccctagtgga cgaatcgcct tcgaggtacg cgggcgaggt
     1921 acgcaggtcg tcaaagtgta ttaactcttt ggtccgtacc acctgggcag tggcgctgcc
     1981 aggatgaggt cgccaccgtc ccattcgcag acgcatctct gcgtattcgg gtggaacatt
     2041 gtaagatgca gacctatgga ctacttcgcc atcgtaaaga ttcagagtac cgaacttggc
     2101 gccgcacagg cgagttgcgt ttgccagaat ggcttgaaac actggctcca gtccggccgg
     2161 cgagcgtgaa atgatcgtca acacttcaga tgtcgccgtt tgctgctcca gcgactgcct
     2221 cagttcggac aggagtcgcg cattctcaat cgcaatcacc gcctggtcag caaaacccct
     2281 cattaggtcg agttcgattt gttggaatgg acgcacctcg ctccgagtga cgaccatcac
     2341 gccaatcgcc tcgccctgcc tggacagcgg cgtgaatgcc tgcgagccga gtggcagggc
     2401 gggcaacaca cgggcgattt ctccgccttc attcggcagg acggcgcggc ggccctcaat
     2461 atggatttgg cgattgtcaa agacagagcg cgccgctgga tccggcgatt caagtgggaa
     2521 gtcaggcaaa atttctcgaa ctttggctgc gctgggaccc gccgcaccga tgacgcgaag
     2581 gacattgccc tcaacgcgcc taaggctgac gctggctgca ttgaacatcc gcacggccgt
     2641 ttcggcgatg gtgtcgagcg cgcgcgtgct gtcctcggga gtactggcaa ttacccgtag
     2701 gatgtcggct gtcgcggttt gacggtcgag gctttcgcgt agctcgttga gcagtcgcgt
     2761 gttctcgatg gcgatgacgg cttgggcggc gaaggtagta accaactcga tttgattggc
     2821 atcaaacggc ctgactgtcg ggcgcgtcag cactatcaca ccaacgggat ccccttctct
     2881 caacagcgga actccgagcg ctgtccggta accgccccgt ctctgagcct caaggaatgt
     2941 gtattctaga tcagcgagaa catcgggaat gtggacggtg cgcctctcga gcaccgcacg
     3001 cgctgcaatc gtaccacgac ccggtctgat cgtttgctgc ttcagatatt ctatccattc
     3061 cggtatgtcg ccgctgccgg aagcacaacg ataaccgtca ccctgtctta gaaagataaa
     3121 cgagtgatca gcttggcaaa gtcga
//
LOCUS       DATLJT010001172         8081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1003309,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001172 DATLJT010000000
VERSION     DATLJT010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003309"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VM910_26970"
     CDS             complement(<1..187)
                     /locus_tag="VM910_26970"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493481.1"
                     /note="catalyzes the formation of methanethiol and
                     2-ocobutanoate from L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HVI66198.1"
                     /translation="MVKPFPSKTFIGNHALHPETLMLNYGYDPQLSEGAVKPPVFLTS
                     TFVFRTAEDGQDFFDFVS"
     gene            complement(259..1926)
                     /gene="hutU"
                     /locus_tag="VM910_26975"
     CDS             complement(259..1926)
                     /gene="hutU"
                     /locus_tag="VM910_26975"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVI66199.1"
                     /translation="MNRRLDNERAIRAPRGSELSAKSWLTEAPLRMLMNNLDSEVAER
                     PSELVVYGGIGRAARDWESFDRIVASLRKLESDQTLLVQSGKPVGIFRTHPDAPRVLI
                     ANSNLVPHWATLDHFNELDRLGLMMFGQMTAGSWIYIGSQGIVQGTYETFVEVGRRHY
                     GGNLAGKWILTAGLGGMGGAQPLAATMAGASLLAIECQPSRIEMRLRTSYLDRQASTL
                     DEALSIMAQAAQTKKPVSVGLLGNAAEIFPELVRRGVRPDIVTDQTSAHDPINGYLPK
                     GWTLAEWEASRAADPKVVEIAAKTSMVDHVRAMLDFNAQGIPTLDYGNNIRQMAKDMG
                     LKNAFDFPGFVPAYIRPLFCRGVGPFRWAALSGDPEDIFRTDAKVKELMPNDRHLHNW
                     LDMAKARIKFQGLPARICWVGLGDRHRLGLAFNEMVARGELKAPIVIGRDHLDSGSVA
                     SPNRETEAMRDGSDAVSDWPLLNALLNCASGATWVSLHHGGGVGIGYSQHAGMVIVAD
                     GTPEAARRLERVLWNDPASGVMRHADAGYETAIACAQANALDLPSVL"
     gene            complement(1923..3500)
                     /gene="hutH"
                     /locus_tag="VM910_26980"
     CDS             complement(1923..3500)
                     /gene="hutH"
                     /locus_tag="VM910_26980"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVI66200.1"
                     /translation="MTHRDASIVVAPGKVSLDDLARVLAGAAVMLDPSFWPRVEAASS
                     IVAKAARADAPVYGINTGFGKLASTRIAADQTALLQRNLIVSHCCGVGAPTPEPIVRL
                     MMALKIVSLGRGASGVRRDVIEQLQTMLARGVCPLVPQQGSVGASGDLAPLAHMTAVM
                     IGEGQAIVDGRAVPGREALAAAGLSPLTLGPKEGLALINGTQFSTAYAVSGLLRAHRL
                     ACSALVTGALSVDAAMASTAPFRAEIQELRGHAGQVAVGAALTALLAGSDIRQSHLEG
                     DERVQDPYCLRCQPQVGGAALDLLTQAARTLTIEANAVTDNPLVLVETGEIVSGGNFH
                     AEPVAFAADQIALAMSEIGATSERRIATLVDPALNFGLPPFLTPDPGINSGFMIAEVT
                     AAALYAENKQRAVACSIDSTPTSANQEDHVSMAAHAARRLSDMADNLATILGIELLVA
                     AQGITLRLPHSTSAPLAAVIAALREQVPELRADRYMADDLAKATALVEAGVLPAAAIS
                     ALQTNPFPILAERGRLS"
     gene            complement(3497..4711)
                     /gene="hutI"
                     /locus_tag="VM910_26985"
     CDS             complement(3497..4711)
                     /gene="hutI"
                     /locus_tag="VM910_26985"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVI66201.1"
                     /translation="MAERFDRIWHNARLVTMREDLPDLGVIERGLIAARDGRIVFAGA
                     RSDVPSDAEAAERIDCAGRWVTPGLVDCHTHLVFGGNRAHEFELRLKGASYEEIARAG
                     GGIVSTVAATRGASEAELVADALPRLDALIGEGTTTVEIKSGYGLNTETEMRQLSAAR
                     SLGRSRTVAIQTSFLGAHALPPEAGGDKDRYIDLVCREMLPAMARAGLADAVDAFMEG
                     IAFSRDQTVRVFQAAKAQGLPVKLHADQLSNLGGAALAAKFSALSADHLEHTDGAGVA
                     AMAEAGTVAVLLPGAFYFIRETQKPPVELFRAHGVNMALATDCNPGSSPLTSLLLAMN
                     MGATLFRMTVAECLAGVTREGARALGILGETGTLEPGKWCDLAIWDIDRPAELVYRIG
                     FNPLHSRVWRGR"
     gene            4798..6156
                     /locus_tag="VM910_26990"
     CDS             4798..6156
                     /locus_tag="VM910_26990"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HVI66202.1"
                     /translation="MSTLHFGSALLPSGWADDVQVMVTDATITKVTTGVAPAARDERH
                     QLAVPGMANLHSHAFQRGMAGLAEMRGNTADTFWTWRETMYRFALEMTPEDVESVATL
                     LYVEMLEQGYTRVGEFHYLHHDRDGSPYANPAEMATRIVRAAEITGIGLTLLPSFYAH
                     GSFGGAAPHAGQRRFICSTDQFARLIAATRVAVGTLPGANIGVAPHSLRAVTPEELGD
                     ILALAEAEPIHIHAAEQIREVEDCVAWSGRRPVEWLLEHTAVDQRWCLIHATHMTTAE
                     TAALASSGVVAGLCPVTEASLGDGIFAAREFVGAGGRFGIGTDSNVLVSVVDELRQLE
                     YGQRLKHRERNVLAGGSGVSTGRALFDAALAGGARALAQRTAGIEPGARADIVTLDTA
                     HPSLAGRRGDAVLDGWIFAAGAGAVDSVWAGGHKVVEGGRHVLRPKARDQFNTTIRRL
                     LA"
     gene            6153..6878
                     /gene="hutC"
                     /locus_tag="VM910_26995"
     CDS             6153..6878
                     /gene="hutC"
                     /locus_tag="VM910_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HVI66203.1"
                     /translation="MSLAADRDKPTLYKQIRRDIERRILTGEWPPGHRIPFEHQLMAR
                     YGCSRMTVNKALSELAQADLIERRRRAGTFVRRPQHLSAVLKIADIRAEITALGRSYG
                     YQLIHCSRRAANAADRGRLGVRKSGKVIAIACRHSADNVPFAVEDRLIDLEAVPEAAA
                     ADFAIEPPGSWLLHHVPWTEAEHTISAVVADDRTAAALDIAVGAPCLVIDRHTWRNAR
                     TVTAVRLLYPGESHRLVARFKGG"
     gene            7177..7536
                     /locus_tag="VM910_27000"
     CDS             7177..7536
                     /locus_tag="VM910_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66204.1"
                     /translation="MKKLLSCVIAAGCLVALASAAQARSAYDGSWDLIFVTQRGACDA
                     TYNFSVNITNGIVTHPNLVRFRGYVARSGAVRASVTVQDKFASGSGRLSSNSGRGTWS
                     GYSGSSRCSGYWTAQRN"
     gene            7813..>8081
                     /locus_tag="VM910_27005"
     CDS             7813..>8081
                     /locus_tag="VM910_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI66205.1"
                     /translation="MNTLPLKNVSLALQGGGAHGAFVWGVIDRLLEDGRIGIEAISAT
                     SAGAMNGVALASGMAIGGPVAARETLYNFWREVSRLDSIFDVYSPL"
BASE COUNT         1521 a         2585 c         2519 g         1456 t
ORIGIN      
        1 ccgagacgaa atcgaagaag tcctgcccat cttcggcggt cctgaagacg aaggtcgagg
       61 tcaggaagac cggtggtttg accgctcctt ccgacagttg cggatcgtag ccgtagttca
      121 gcatcagcgt ttcgggatgc aacgcgtgat tgccgatgaa ggtctttgac ggaaacggtt
      181 tcaccatggc ctgtctcgtt gtcttgaggg aaggttgaac ccggcacgca ccgatggcgg
      241 tctgaaatct ccgatgcgct acaacacact gggcagatcg agcgcgttgg cttgggcgca
      301 tgcaatcgca gtttcatagc ccgcgtcggc gtgacgcatg acgccgctcg cgggatcgtt
      361 ccacagcacc cgttcgaggc ggcgggcagc ctccggggtc ccgtcggcga caatcaccat
      421 gccggcgtgc tgcgaatatc caatacctac gccgccgccg tgatgcagcg aaacccaggt
      481 cgccccactg gcacagttga gcagcgcatt gagcagcggc caatcggaca ccgcatccga
      541 gccatcgcgc atcgcttcgg tctcacggtt cggactcgca accgagccgc tgtcgagatg
      601 atcgcgtcct atcacgatcg gcgccttcaa ttcgccgcgc gccaccatct cgttgaaggc
      661 caaccccagc cggtggcgat cgccgaggcc gacccagcaa atccgggccg gcaagccctg
      721 gaacttgatc cgtgcttttg ccatatccag ccagttgtga agatgccgat cgttcggcat
      781 tagttccttc accttggcgt cggtccggaa gatatcctca ggatcgcccg acaacgcagc
      841 ccaccggaac ggcccgacgc cccgacagaa cagcgggcga atataggccg ggacgaaccc
      901 gggaaaatcg aaggcattct ttagccccat atccttcgcc atttggcgaa tgttgtttcc
      961 gtaatcgagc gtgggaatac cttgagcgtt gaagtccagc atcgcccgca cgtgatcgac
     1021 catggatgtc ttggccgcga tttcgaccac tttcggatcg gcggcgcggc ttgcctccca
     1081 ctcggcaagc gtccatccct tcggcagata gccgttgatt ggatcgtgcg cgctggtctg
     1141 atcggtgacg atgtcgggcc gtacgccgcg ccggaccagt tcgggaaaaa tctcggccgc
     1201 attgccaagc aggccgaccg agaccggctt cttggtctgc gcggcctgag ccattattga
     1261 aagcgcctcg tcaagagtgc tcgcctgccg gtcgagataa ctcgtgcgca gccgcatctc
     1321 gatgcggctc ggctggcatt cgatcgcaag cagcgacgcc cccgccattg tcgcagcaag
     1381 cggctgggcg cctcccatgc caccgaggcc cgccgtgagg atccatttgc ccgcgaggtt
     1441 gccgccgtaa tgacgccgac ccacctcgac aaacgtctca taggtcccct gcacaatgcc
     1501 ctggctgccg atgtagatcc aggatccagc cgtcatctgc ccaaacatca tcaagccgag
     1561 ccggtccagc tcgttgaagt gatcgagtgt cgcccagtgc ggcaccagat tcgagttcgc
     1621 gatcaaaacg cgcggcgcat ccggatgggt gcggaaaatt ccgaccggct tgcccgactg
     1681 aaccaatagc gtctggtcgc tttcgagctt gcgcaatgag gcgacgatcc ggtcgaagct
     1741 ttcccaatcc cgcgctgcac gaccgattcc gccgtagacg acgagctcgc tcggccgttc
     1801 ggcaacctcg gaatcaaggt tgttcatgag cattcgaagc ggcgcttcgg tcaaccagct
     1861 cttggcgctg agctcggatc cgcggggcgc acggatagca cgctcgttgt ccagtcggcg
     1921 gttcatgaca gacgacccct ctcggcaagt atcggaaacg gattggtctg aagcgccgag
     1981 atcgcggcgg ccggcaatac gccggcttcg accagcgcag ttgccttcgc aagatcgtcg
     2041 gccatgtaac gatcggctct cagctccggc acctgctcgc gcaacgcggc gatgaccgcc
     2101 gcgagcgggg cgctggtcga atggggcaag cgcagggtga tgccttgcgc cgcgaccaac
     2161 agctcgatcc ccagaatcgt ggccaggttg tccgccatgt cggacagccg gcgcgcggcg
     2221 tgcgcagcca tcgagacgtg atcttcctgg ttggcgctgg tcggcgtcga atcgatcgag
     2281 caggcaaccg cgcgctgctt gttctcggca tagagtgcag ccgccgtcac ttccgcgatc
     2341 atgaaaccgg agttgatgcc gggatcggga gtgaggaacg gcggcagccc gaaattaagg
     2401 gcgggatcaa caagggtagc tatgcgccgc tcgctggtcg cgccgatctc cgacatcgca
     2461 agcgcaatct ggtccgcggc aaaggccacc ggttcagcat gaaaattccc acccgagacg
     2521 atctctccgg tctcgacgag caccagcggg ttgtcggtga cggcgttggc ttcgatcgtc
     2581 agcgttcgag cggcctgcgt gagcagatcg agggctgcgc ctccgacctg cggctggcaa
     2641 cgcaggcaat agggatcctg cacgcgctcg tcgccttcga gatgcgactg gcgaatgtcg
     2701 ctgcccgcga gcaaggccgt cagggccgcg cctaccgcga cttgcccggc atgcccgcgc
     2761 aattcctgga tttcagcgcg aaacggcgcc gtcgaagcca tagccgcatc gaccgacaag
     2821 gcgcctgtta cgagcgccga acaggccagg cgatgggcgc gcagcaaccc ggagacggca
     2881 taggccgttg aaaactgagt gccgttgatg agggcgagac cttccttcgg tccaagcgtt
     2941 agcggtgaga gaccggcggc agcgagcgcc tcacggcccg gcacagccct gccatcgaca
     3001 atcgcctgac cttcgccgat catgacggct gtcatatgcg caagcggcgc cagatcgccg
     3061 gaggcgccta ccgagccctg ctgcggcacg agcggacaaa cgccgcgtgc gagcatggtc
     3121 tgcaattgct cgatgacgtc acggcgcacg cccgaagcac ctcgcccgag cgagacgatc
     3181 ttcaaggcca tcatcaggcg aacgatcggc tcgggcgtag gcgcccctac tccgcagcaa
     3241 tgcgacacaa tgagattgcg ctgaagcaac gccgtctggt cggcggcaat gcgcgtcgat
     3301 gccagttttc cgaatccggt attgatgcca taaacgggag cgtccgcgcg agccgctttc
     3361 gcgacaatgc ttgaagccgc ttcgacgcgc ggccagaatg acggatcgag catcacagcc
     3421 gcgccggcga gcactcgcgc cagatcgtcg aggctgacct ttccgggcgc aacgacaatt
     3481 gatgcgtccc gatgggtcat cgccccctcc acacccggct gtgcagggga ttgaagccga
     3541 ttcggtagac aagctcggca ggccgatcga tgtcccagat cgcgagatcg caccatttgc
     3601 cgggctcgag cgtaccggtc tcgccgagaa taccgagcgc gcgggcccct tcccgcgtca
     3661 cgcccgcgag gcattccgca acggtcatcc tgaacagcgt cgcgcccatg ttcatggcga
     3721 gtagaagcga agtgagcggc gagctgccgg gattgcaatc ggtagccagc gccatgttga
     3781 cgccgtgggc gcggaatagc tcgaccggcg gcttctgcgt ctcgcggatg aagtagaaag
     3841 caccgggcag caggaccgcc acggtgccgg cctccgccat ggcggcaacg ccggcgccat
     3901 cggtgtgctc cagatgatcg gcggatagag cggaaaattt cgcagcaagc gccgctccgc
     3961 ccaggttcga taactgatcg gcgtgaagtt tgaccggcaa tccctgcgct ttcgccgcct
     4021 ggaatacgcg caccgtttga tcgcgtgaga aggcaatgcc ttccatgaac gcatccacgg
     4081 catcagcaag cccggctcga gccattgccg gcagcatctc ccggcaaaca agatcgatgt
     4141 atcgatcctt gtcgccaccg gcttcgggcg gcagtgcgtg cgcaccgaga aacgacgtct
     4201 ggatcgcgac cgtgcggctg cgaccgaggc tacgggcggc cgacaactgc cgcatctcgg
     4261 tttccgtgtt aaggccgtaa ccggacttga tctcgaccgt cgttgtaccc tcgccgatca
     4321 gcgcgtcgag cctcggcaag gcatcggcga cgagctcagc ctcgcttgcg ccccgcgtcg
     4381 ctgccacggt cgagacgatg ccgccgcccg cgcgcgcaat ctcttcgtag cttgcaccct
     4441 taagtcgcag ctcgaactcg tgtgcacggt ttccgccaaa caccaagtgg gtgtgacaat
     4501 ccaccaatcc gggcgtgacc caccgccccg cacaatcgat ccgttcagcc gcctcggcat
     4561 ccgatgggac gtccgagcgt gcaccggcaa agacgatgcg accatcgcgc gccgcgatca
     4621 ggccacgctc gatcacaccg agatcgggca gatcctcccg catcgtgacg agccgggcat
     4681 tatgccagat acgatcaaag cgctcggcca tgcagctttc cccctggcgg cagaagcttg
     4741 acgcgtcgat atgtctatac atatgatcgt gccgctctgt ccagccgggc tgaaaacatg
     4801 tccaccctgc attttggctc cgcgctgctt ccctcgggat gggcggatga cgtgcaggtg
     4861 atggtgacag atgcgaccat caccaaggtt acgacgggcg tggcgccggc agcccgagat
     4921 gaacgccacc aactggccgt tcccggaatg gcgaacctgc atagtcacgc ctttcagcgc
     4981 ggcatggcgg gccttgccga aatgcgcggc aacacggccg atacgttctg gacatggcgc
     5041 gagacgatgt atcgcttcgc cctggagatg acaccggaag acgtcgaaag cgtcgcgacc
     5101 ttgctctatg tcgagatgct cgagcagggc tatacgcgcg tcggcgaatt ccactacctc
     5161 catcatgatc gcgacggctc gccctatgcc aaccccgcgg agatggcgac gcgaatcgtg
     5221 cgagctgccg agatcaccgg catcggactg accctgctac cgagtttcta tgcacatggc
     5281 tcgtttggcg gagccgcacc gcatgccggc cagcgtcgct tcatctgctc gaccgatcag
     5341 ttcgccaggc tgattgccgc gacacgcgtc gccgtgggta cgttgccggg agccaatatc
     5401 ggcgtcgcgc cgcacagcct gcgggcggtg acgccggagg aactggggga catcctggct
     5461 ctggctgagg ctgagccgat tcatatccac gccgccgaac aaatcagaga ggtcgaggat
     5521 tgcgtcgcct ggtccggtcg acggccggtt gaatggctgc tcgaacatac ggcagtagat
     5581 caacgctggt gcctcatcca cgcgacccac atgactacag cggaaaccgc cgcgcttgcc
     5641 agcagcggcg ttgtggcagg cctctgcccc gttaccgagg cgagcctggg cgatggcatt
     5701 ttcgccgccc gcgaattcgt cggcgccggt ggccgcttcg gcatcggcac ggattccaac
     5761 gtcctggtca gcgtcgtcga tgagttgcgt cagctcgaat atggccagcg cttgaaacac
     5821 cgcgagcgca atgtgctggc tggcggttcc ggcgtctcga ccggtcgtgc gctgttcgat
     5881 gctgcactcg ccggtggcgc ccgggcgctg gcgcaacgga ccgccggaat agagccgggt
     5941 gcgcgcgccg acattgtcac gctcgacacc gcgcatccat cgcttgcggg gcgccgcggc
     6001 gatgccgtcc ttgacggctg gatttttgca gcgggtgcgg gtgctgtcga tagtgtctgg
     6061 gcgggcgggc ataaggtggt tgagggcgga cgtcatgtgc tccgcccgaa ggcgcgcgat
     6121 caattcaaca caacaattcg gaggctcctg gcatgagcct cgccgccgat cgggacaagc
     6181 cgacactgta taaacagatc cggcgcgaca tcgaacgccg aattctgacc ggcgaatggc
     6241 caccgggtca tcgcatcccg ttcgagcacc agctcatggc gcgatacggc tgctcgcgaa
     6301 tgacggtgaa caaagcgctg tcggaactgg cgcaagccga tttgatcgaa cgcaggcggc
     6361 gggccggcac attcgtccgt cgtccccagc atctgtcggc ggtgctcaag atcgctgaca
     6421 ttcgtgcgga gatcactgcg ctgggccgca gctacggcta tcagctcatc cattgctcgc
     6481 gccgcgccgc aaacgctgcc gaccgcggac gcctcggggt acggaaatcc ggcaaggtca
     6541 tcgcaatcgc ctgccgtcac agcgccgaca acgtgccatt tgccgtcgag gacaggctga
     6601 tcgacctcga ggcggtgccg gaagccgccg ccgcggactt tgcgatcgag ccgcccggct
     6661 catggctgct ccaccatgtc ccatggacgg aagccgagca cacgatcagc gccgtggtcg
     6721 cggacgatcg cactgcggct gcgctggaca ttgcggtcgg cgcaccttgt ctcgtgatcg
     6781 accggcacac ctggcgcaac gcacggaccg tgaccgcggt gcgcctgctc tatccgggcg
     6841 aatcccatcg gctggttgcg cgcttcaaag gaggctgaga gcaggatgac agcgcgtccg
     6901 cgcaaattca aaggatcgac gcgcccgagg gatacgggcc ttcagcctct tcaacatgag
     6961 gtgaggatgc cgcgatgccg ttgtccgcag atgcggtcgt catcagatag gacgatgacc
     7021 tgcacgtgag gccgcgcgtc ggcggacgaa cggcaaactc gaaacatttg gagcaaattt
     7081 ggacaagcat cttgtcctat gttgccgaca atccattgta gtctggcctc taaggaggct
     7141 cgcctcggct ttcatgacga cgacctggag gaaacgatga agaagcttct gtcttgcgtg
     7201 atagctgccg gctgtcttgt cgccctcgcc tccgccgcac aggcgcgctc cgcgtatgat
     7261 ggttcgtggg acctcatttt cgtgacgcaa agaggcgctt gcgatgcaac gtataatttc
     7321 tccgtcaaca tcaccaacgg aattgtcacg catcccaacc tggtcaggtt cagaggctac
     7381 gtggcgcgct ccggcgcggt tcgtgcctcg gtgaccgttc aagacaaatt cgcctccggc
     7441 tcgggtaggc tctcgagcaa ttctggtcgc gggacgtgga gcggctattc gggaagttcc
     7501 cgctgctcag gctattggac cgcgcagcga aactgaaatc gagggatctc cgcaaaactg
     7561 tccaaacccg gcaaggtcgt tcgcgagaag gacttcgcag atcgataaaa gtggccagca
     7621 gcgtgttggc tctatgcgga atcggcagcc gaagccggcg acccgtccgg ctgccgaatt
     7681 tgcatagccg gaccccgctt catcatgcac cgtgtgtgtt ctgcatcgat cgagtcaggg
     7741 attgcgcgcc cccaaaccat ttcagggttt cggccgatcg caaacgccac tgctgcctgg
     7801 gatgctacag atgtgaacac gcttcctttg aaaaacgtaa gtctcgcgct tcagggtggc
     7861 ggggcccacg gggcttttgt ctggggggtg atcgaccgct tgcttgagga cggtcgcatc
     7921 ggaatcgagg cgatcagcgc taccagtgcg ggcgcgatga atggggttgc cctcgcctcc
     7981 ggcatggcga tcggcgggcc cgtggccgcc agggaaaccc tttacaattt ttggcgtgag
     8041 gtttcgcggc tcgattcaat attcgacgtt tactcgcctc t
//
LOCUS       DATLJT010001173         5115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2153073,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001173 DATLJT010000000
VERSION     DATLJT010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2153073"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VM910_27010"
     CDS             complement(<1..693)
                     /locus_tag="VM910_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66206.1"
                     /translation="MAGRIQITIARIALVVAILALWEILSRTGLVNPRLLPSASDTLS
                     TLADLLQRGSVRSDLLVTATEVLVAFAIAVPLGCLIGYLIAEYRYFAEVMKPLLFFAF
                     SIPKSIFLPMFILVFGVGFAEKVGFGFFSTIFIVIMSTTTAVESVKVEYLTVARSYGA
                     TSAQTAFRVYLPSMLPVLLEALRISMIFNLTGIILAEMYASRDGIGHQIATWGENFQM
                     KQLLAGVVMIAVI"
     gene            complement(703..1377)
                     /locus_tag="VM910_27015"
     CDS             complement(703..1377)
                     /locus_tag="VM910_27015"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66207.1"
                     /translation="MSKIVANASMGNFGWQLMVPAKSTLEVKDLNGKKVAITAAGSGS
                     DLLALWTIQDRKIDFTRVPVGGGGIVPNLLAGNVDAAVVYSPLSFQILKSGEARTILD
                     YSTAVPPNLTAGWIVLDKFAEANPQVVQKALNALYGAVQFMRNNRDVTVKLIADLYEM
                     PTEIAALEYENSIMKLETYGSMAGPNVKDAVQLSLDLAKLGGLKDVVPAEEVYSTQFK
                     PVPTKP"
     gene            complement(1438..1671)
                     /locus_tag="VM910_27020"
     CDS             complement(1438..1671)
                     /locus_tag="VM910_27020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66208.1"
                     /translation="MTVSRRPALALLAIAALLTASAVARAETIRVGLPTKTYWPTTVA
                     ETAVRQKLFEKEGITAELTIYRSGAETFEGMAA"
     gene            complement(2164..2508)
                     /locus_tag="VM910_27025"
     CDS             complement(2164..2508)
                     /locus_tag="VM910_27025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66209.1"
                     /translation="MDSNDRRAETGREGLTRRAVLTTATVAAGALAGPAGAAAETAHG
                     GDFGQPFVEFYMPPGVLTLEQRSDIVAAFTDVVLAATKQSLDPAKRLYVEIIETAEGG
                     FGVNGQPFVPKK"
     gene            complement(2585..2776)
                     /locus_tag="VM910_27030"
     CDS             complement(2585..2776)
                     /locus_tag="VM910_27030"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI66210.1"
                     /translation="MPVAKIYVPEGALTPDQRREIIKGIHDAINRIEKRPPNAPTYVI
                     VTEIPAGNWGNAGTVYRPG"
     gene            complement(2882..3583)
                     /locus_tag="VM910_27035"
     CDS             complement(2882..3583)
                     /locus_tag="VM910_27035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66211.1"
                     /translation="MRQPEPIADYADTLARRLRFDIALARRVRTEVEDHLRESADELG
                     GASPDNQLLAIAGFGDPDDLARQYAADCLLAQIQQVSIAVALAVIGVFLAMGSRVVWY
                     SFTQWESNALLKRLVPIALPIDHYASLIAIGCAALGFGYLVTRRTPADFHAVYRRQLG
                     VGLLLCGGAALALLAVVGVELVVTSCRIAAAMPSPAILVPALTVLAEIALVIAVIFRI
                     AAAIRYAVIAGTLAK"
     gene            complement(3558..3908)
                     /locus_tag="VM910_27040"
     CDS             complement(3558..3908)
                     /locus_tag="VM910_27040"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI66212.1"
                     /translation="MEGEMLKGHLDMIVLAALAGAPAHGYAIIQEIRRRSGGAFDLPE
                     GTIYPALRRLEKAGLLASRWVVAESGRKRRVYTLTRRGRLALADHRAVWQRFIDAVGG
                     LFGSMDHATARADR"
     gene            4147..>5115
                     /locus_tag="VM910_27045"
     CDS             4147..>5115
                     /locus_tag="VM910_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI66213.1"
                     /translation="MTDTNNNDAPHDATEFDYVIVGAGSAGCVLANRLTADGKHSVLL
                     LEAGPKDTNIWIHVPLGYGKLFKEKSVNWMYQTEPEPGLHGRQVFQPRGKVLGGSSSI
                     NGLLYVRGQHEDYDRWRQHGNAGWGYDDVLPYFKRAENQQRGADDYHGVGGPLSVSDW
                     RHADPLSEAFVKAAVEAGIPFNPDFNGARQEGAGYFQTTTRRGRRASSAYSYLRPARS
                     RGNLHVETAALAQRVLFEGRRARAVEYRQNGGLRTARARKEVVVSGGAYNSPQLLQLS
                     GVGPAELLKQHGIDVVLDARGVGNDLQDHLQVRLVTRCAQSVTLNDV"
BASE COUNT          953 a         1588 c         1650 g          924 t
ORIGIN      
        1 aatcacggcg atcatgacga ccccggccag aagctgcttc atctgaaaat tctcgcccca
       61 ggtcgcgatc tggtgaccga tgccgtcgcg cgaggcatac atctcggcca ggatgatgcc
      121 ggtcaggttg aagatcatgg aaatgcgcag ggcttccaac agcaccggca gcatgctcgg
      181 cagatagaca cggaacgccg tctgcgccga tgtcgcgcca taggatcgtg ccaccgtgag
      241 gtactcgacc ttgaccgact cgaccgccgt cgtcgtcgac atgatcacga tgaaaatcgt
      301 cgaaaagaaa ccgaacccga ccttctcggc gaagccgacg ccgaacacca ggatgaacat
      361 cggcaaaaag atcgatttgg gaatggagaa ggcgaagaac agcagcggct tcatgacttc
      421 ggcaaagtag cgatattccg cgatcagata gccgatcagg cacccgagcg gaacggcgat
      481 cgcaaacgca actagcacct cggttgcggt gaccaggagg tcgctgcgca cgctgccccg
      541 ctgcagcaaa tcggcgagcg tcgagagcgt gtccgacgcc gaaggcagca gccgcggatt
      601 gacgaggccg gttcgcgaga ggatctccca cagcgcaagg attgcgacga caagcgcgat
      661 gcgggcgatc gtgatctgga tgcggccggc catcgcaagt gctcaaggtt tggttggcac
      721 cggcttgaac tgtgtcgaat aaacctcctc cgccggcacg acgtccttca gcccgccaag
      781 cttggcgaga tccagcgaaa gctgcacggc gtccttgaca ttgggaccgg ccatgctgcc
      841 gtaggtctcg agcttcatga tcgagttctc gtattcgagg gcggcgatct cggtcggcat
      901 ttcatagaga tcggcgatca gcttgaccgt gacgtcgcgg ttgttgcgca tgaactgcac
      961 tgcgccgtag agcgcgttga gcgccttctg cacgacttgc ggatttgctt cggcaaactt
     1021 gtcgagcacg atccagcccg cggtgaggtt cggcggcacg gcggtcgaat agtcgagaat
     1081 ggtgcgcgcc tcgcccgatt tcaaaatctg gaaggagagc ggcgaataga ccacggccgc
     1141 atcgacattg ccggcgagca ggttcggcac aatgccgccg ccgccgacgg gcacgcgggt
     1201 gaaatcgatc ttgcggtcct ggatcgtcca tagcgccagc agatcggagc ccgatccggc
     1261 cgccgtgatc gcgaccttct tgccgttaag gtccttcacc tccagcgtcg atttggccgg
     1321 caccatcaat tgccagccga aattgcccat cgaggcattg gccacgatct tgctcatcac
     1381 gcccttcttg cggccggccg agaccagcga gggcggatcg agaatgatgt cggcggctca
     1441 ggccgccatg ccttcgaagg tttcggcgcc cgagcggtag atcgtcagct ccgcagtgat
     1501 gccttccttc tcgaacagct tttgccgcac tgcggtctcg gcaaccgtcg tcggccaata
     1561 tgtcttggtt ggcaggccga cgcggatggt ctcggcgcgg gcgacggccg atgcagtgag
     1621 cagcgcggcg atggcaagta acgccagagc agggcggcgt gagacggtca ttgttggatt
     1681 tcctcccgaa cttgcgcgca ttgtcgcttt gcgcttccat catcttcaat ggaaaagcaa
     1741 gccttttgca attgaccgtt cggtgtcccc tcgttacctc ctcccactcg acaggcccac
     1801 gtcgcatgac gccaacgcgt tgtcgcgtcg cgcgcgactt gtccgggtta ttcggcctcg
     1861 ccggttgcgc gccgggcacc cgctgcggcc acctcggtcc cggcgtcgtt ctttagctga
     1921 ttgaaggaaa cgggcatgtc ggcgggcgag gtcgaggacc tctaccgccg ccatggccag
     1981 aaggggcatt cgggcatccc gcagaacatt cgcgcgctgg aagcgcggcg ccgtcggcga
     2041 gcacgtcgcc ggcattctcg tgggcgcgcg tctgtgtcat accgagcggt gaggagatgt
     2101 tgattctcct ccacactgcg cggctgttct cgtcggcgca ggagctgcgg gcggcctcgc
     2161 ggactacttc ttcggcacga agggttgacc gttgacgccg aagcctcctt ccgcggtctc
     2221 gatgatttcg acatacagcc tttttgccgg atcgagcgac tgcttggtcg cggccagcac
     2281 cacgtccgtg aatgcagcaa cgatgtcgct gcgttgttcc agcgtcagca cgcccggcgg
     2341 catataaaat tcgacgaacg gctgaccgaa atctccaccg tgcgcggttt ccgctgccgc
     2401 gcctgccggg ccggccaggg cgccggcggc gacggttgcc gtcgtcagca cggccctccg
     2461 agttagcccc tcgcggccgg tttcagcgcg acgatcgttc gagtccattg tcagacctcc
     2521 ttgaaaaaga cggcgccgca cgtcatgggc cggcgctgcg atacagattg cggccttggg
     2581 acgctcagcc cgggcgatag acggtgccgg cattgcccca attgcctgcc ggaatctccg
     2641 tgacgatcac ataggtcggc gcgttgggcg gccgcttttc gatccgattg atcgcgtcgt
     2701 gaatgccctt gatgatctcc cggcgctggt ccggcgtcag tgcgccttca gggacataga
     2761 ttttggcaac cggcattgca gtcctccata agcatcgtgt tgctatacta tgtaatacga
     2821 tgtatagagt caatgctggt gcgttcacaa caaattgtgc tggcggacga gactggcggt
     2881 gctacttcgc cagcgtccct gcgatgacgg catagcggat tgcggctgcg atcctgaaga
     2941 tgacggcaat gaccagggcg atttcggcca gcaccgtcag cgctggaacg agtatcgcag
     3001 gcgagggcat cgcggccgcg atgcggcaag aggtcacgac gagctcgacg ccaaccaccg
     3061 ccagcaacgc aagggcagcg ccgccgcaca ggagaagtcc tactccgagc tgccggcggt
     3121 agacagcatg aaagtcggcg ggcgtccggc gagtgacgag atagccgaag ccgagagctg
     3181 cgcagccaat cgctatgagg gatgcgtagt ggtcgatcgg cagcgcgatc gggacgagac
     3241 gcttcagtaa cgcattgctc tcccattgcg tgaagctgta ccagacaacg cgacttccca
     3301 tcgcgagaaa tacgccgatc acggcgagcg cgaccgcgat gctcacttgt tgtatctgtg
     3361 cgagcaggca atcggcagcg tattgacggg cgaggtcgtc gggatcgccg aagccggcga
     3421 tcgccaaaag ctggttgtcc ggcgacgcgc cgcccagttc gtcggcgctc tcccggagat
     3481 gatcttcgac ctccgtgcgc acccggcgcg ccagcgcgat gtcgaagcgc aggcggcgcg
     3541 ccagcgtgtc ggcatagtca gcgatcggct cgggctgtcg catggtccat actcccgaac
     3601 aagcctccca ccgcatcgat gaagcgctgc cacaccgcgc ggtgatcggc aagcgcgagc
     3661 cggccgcggc gggtcagggt atacacgcgc cgtttgcggc ccgattcggc aaccacccag
     3721 cggctcgcga gcaagcccgc tttctcgaga cggcgcagcg ccggatagat ggttccctcg
     3781 ggcagatcga aggcgccacc gctgcggcgt cggatttcct ggatgatcgc atagccgtga
     3841 gccggcgcgc cggcgagggc cgccagcacg atcatgtcga ggtgaccttt cagcatctcg
     3901 ccttccattg aaagggaggc tatgccttgc gttgcaaggg gtcaatagca gggcggtagg
     3961 atagccgacg gcgatttcgt tgcacttggt ggaggtgaga agcgcgcact cgcgcaggaa
     4021 ccggccttgg tcccgctcac ggcgccggct tttgtgattg accctgttcg acgcccccgt
     4081 tacctgttcg gctcgctcaa ggagatggtc tactgcgcgg ctgacgccgg cccgcgagga
     4141 aaccacatga ccgacactaa caacaatgac gcgccgcacg acgccaccga gttcgattat
     4201 gtcatcgtcg gcgcaggctc ggccggctgc gttctggcca accgattgac ggcggatggg
     4261 aagcattcgg tgttgctgct cgaagcgggg ccaaaggaca ccaacatctg gatccatgtg
     4321 ccactcggct acggcaaatt gttcaaggaa aaatccgtca actggatgta ccagaccgag
     4381 ccggagccgg gccttcacgg acggcaggtt tttcagccgc gcgggaaagt gctgggcgga
     4441 tcgagctcga tcaacggcct gctctatgtc cgcggccagc acgaggatta cgaccgctgg
     4501 cgtcaacacg gcaatgccgg gtggggttac gacgacgtgc tgccctattt caagcgggca
     4561 gaaaaccagc aacgcggtgc cgacgactat cacggggtcg gcggtccgct gtcggtgtcg
     4621 gactggcgcc atgccgatcc cttgtccgag gccttcgtca aggcggcagt cgaagccggc
     4681 attcccttca accccgattt caacggcgcg cggcaggaag gcgcgggcta tttccagacc
     4741 acgacgcggc gcggccggcg cgcttccagc gcgtattcct atcttcgccc ggcacggagc
     4801 cgcggcaatc tccatgtcga aacggcggcg ctggcgcagc gcgtcctgtt cgagggacgc
     4861 cgcgcgcgtg cggtcgaata ccggcagaac ggcggcttgc gcacggcgcg ggcgcgcaag
     4921 gaagtggtgg tgtcgggggg cgcgtacaat tcgccgcagc tcctgcagct ctccggcgtc
     4981 ggaccggccg agctgttgaa gcagcacggc atcgatgtcg tgctggatgc gcgcggggtc
     5041 ggcaacgatc tgcaggatca tcttcaggtc cgtctggtca cgcgttgcgc gcaaagcgtc
     5101 acgctcaacg acgtc
//
LOCUS       DATLJT010001174         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3532771,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001174 DATLJT010000000
VERSION     DATLJT010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3532771"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VM910_27050"
     CDS             complement(<1..659)
                     /locus_tag="VM910_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HVI66214.1"
                     /translation="MSALFSPIELRGLSLPNRIMVAPMCQYSADHGEATDWHFTHVNS
                     LALSGAAMFCIEATAVEAIGRITHGCLGLWNDACEAALKPIVASVRRHSKTAVAMQLA
                     HAGRKASSQRPWEGGQLIPLDKGGWQTEGPSAVPHKEGEAPPLALDTLGLARVCEAFV
                     TATKRAERLGLDALELHCAHGYLMHQFLSPISNRRTDQYGGSLENRMRFPLEVFDAIR
                     AT"
     gene            complement(776..1846)
                     /locus_tag="VM910_27055"
     CDS             complement(776..1846)
                     /locus_tag="VM910_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HVI66215.1"
                     /translation="MRAGISRRCFLNGAAAGLATLVPPRLAQAQSAATQPPPKQGAVD
                     EYSADRPVSIEVNARQIPSFDPRDHSHVRFGALEYRSGLVLTSSYRGFGGLSAIRLDR
                     KGERFLALSDKGSWFTGRIVYSGREMTGLADVEAAPILGPDGRPVATRGWYDTESIAL
                     DGSMVYVGIERVNQVLRFDFSKGFTRARGEVVPLPPAAKKLPVNKGLEALVMVPKGRP
                     LAGTLIAMSERGLDPQGNLIAFLVGGPTPGQFAVRRTNDFDISDAVLLGGDVLVLERK
                     FSWAAGVGIRIRRIPLESIAPGALVDGPSIFDADLGQEIDNMEGIDAHVTAEGDTVLT
                     MVSDDNFSLIQRTLLLQFTLVE"
     gene            complement(1850..>2273)
                     /locus_tag="VM910_27060"
     CDS             complement(1850..>2273)
                     /locus_tag="VM910_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HVI66216.1"
                     /translation="PWRRSRKNLGLMMREGLLKENIDGEALDWAHRRLLGRAEQRKIL
                     MMISDGAPVDDSTLSVNPGNYLERHLRHVIEEIETRSPVELIAIGIGHDVTRYYRRAV
                     TIVDAEELGGAITEKLAELFSETHGVPPAAQSQRRLHS"
BASE COUNT          413 a          738 c          745 g          377 t
ORIGIN      
        1 gtcgcgcgga tcgcgtcgaa cacttcgagc ggaaagcgca tgcggttctc gagcgagccg
       61 ccgtactggt cggtgcggcg gttcgagatc ggcgacagaa attgatgcat cagatagccg
      121 tgggcgcaat gcagctccag cgcgtcgagg cccagtcgct cggcgcgctt ggttgccgtc
      181 acgaacgcct cgcacacccg cgcgagcccc agcgtatcga gcgcaagcgg cggcgcctcg
      241 ccctccttgt gcggcaccgc ggatggacct tccgtctgcc agccgccctt gtcgagcggg
      301 atcaactggc cgccctccca cggacgctgg ctcgatgcct tgcgcccggc atgggcgagc
      361 tgcatggcaa cagcggtttt cgaatgcctg cgcaccgagg cgacgatcgg cttcagcgcg
      421 gcttcgcagg catcgttcca caggcccagg catccatgcg tgatgcggcc gatcgcctcc
      481 accgccgttg cctcgatgca gaacatcgca gcaccggaca gcgccagcga attgacgtgg
      541 gtgaaatgcc agtccgtggc ctcgccatga tcggcggaat actggcacat cggcgccacc
      601 atgatgcggt ttggaagcga aaggccgcgc agctcgatcg gcgagaacag ggcactcatg
      661 agggatttcc gaatggaggg atgggaaaca ccggcgagat tagagcaagt cgtggagaag
      721 cggctagccg ttttgcgaat ggatcgtctg caggatgatg cgttgggaca tccgcctatt
      781 ccaccagcgt gaactgcagc aagagcgtgc gctggatcag ggagaaatta tcgtcggaga
      841 ccatggtcag caccgtatcg ccctccgccg tcacatgcgc atcgatgcct tccatattgt
      901 cgatctcctg accgagatcg gcatcgaaga tcgaggggcc gtcgacaagc gcaccgggcg
      961 cgatggattc gagcggaatg cggcggatac ggatgccgac gcccgccgcc cacgagaatt
     1021 ttcgttcaag caccagcaca tcgccgccca gcagcacggc atcgctgatg tcgaaatcgt
     1081 tggtgcggcg gacggcgaac tgacccggcg tcggcccgcc gaccagaaac gcgatcaggt
     1141 tgccctgcgg atcgagcccg cgctcggaca tcgcaatcag ggtgccggca agcggccggc
     1201 ctttcggcac catgacgagc gcttccagcc ccttgttgac ggggagcttc tttgccgcgg
     1261 gcggcagcgg caccacctcg ccgcgtgccc gcgtaaaccc cttgctgaaa tcgaagcgca
     1321 gcacctggtt gacgcgctcg atcccgacat agaccatcga cccgtcgagc gcgatcgatt
     1381 cagtgtcgta ccagccgcgg gtcgcaaccg gcctgccgtc tgggccgaga atcggcgcgg
     1441 cctcgacatc ggcaagcccg gtcatctcgc gcccggaata gacgatgcgg ccggtgaacc
     1501 agcttccctt gtcgctcagc gcgaggaaac gctcgccttt gcgatcgagg cggatcgccg
     1561 acaacccgcc aaagccgcga taggacgagg tcagaaccag cccgctgcgg tattccagcg
     1621 cgccgaagcg tacatgcgaa tggtcgcggg gatcgaacga gggaatttgc cgggcattga
     1681 cctcgatcga aaccggcctg tcggccgaat attcgtcgac cgcaccctgc ttcggcggcg
     1741 gttgggttgc agcagattgc gcctgtgcga ggcggggcgg gaccagcgtc gcgagcccgg
     1801 ccgccgcgcc gttcagaaag caacggcggc tgatgcctgc gcgcatggat tacgagtgca
     1861 gccggcgctg gctctgcgca gccggcggca cgccgtgcgt ctcgctgaag agttcggcga
     1921 gcttctcggt gatggcgccg cccagctctt cggcatccac gatggtcacg gcgcggcgat
     1981 aatagcgcgt gacgtcgtgg ccgatgccga tcgcgatcag ttcgaccggc gagcgggtct
     2041 cgatctcctc gatgacgtgg cgcagatgcc gctcgagata attgccggga ttgaccgaca
     2101 gcgtggaatc gtcgaccggc gcgccgtcgg aaatcatcat caggatcttg cgctgttcgg
     2161 cacggccaag caggcgccga tgcgcccagt ccagcgcctc gccgtcgatg ttctctttca
     2221 gaagtccctc gcgcatcatc aggccgagat tcttgcgcga gcgccgccac ggc
//
LOCUS       DATLJT010001175         3599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3762263,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001175 DATLJT010000000
VERSION     DATLJT010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3762263"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VM910_27065"
     CDS             complement(<1..182)
                     /locus_tag="VM910_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI66217.1"
                     /translation="MTTRRASCSCGQLHLTIEGEPSRISMCHCLECQRRTGAAISNQA
                     RFRREQVTFAGKSTAW"
     gene            complement(400..1074)
                     /locus_tag="VM910_27070"
     CDS             complement(400..1074)
                     /locus_tag="VM910_27070"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI66218.1"
                     /translation="MTVELRRDHSPFGGSVAARVLRCPASVGLIEKVPAYLRKVSAYA
                     DRGTALHEAMALLLDEAYSLDDLVGKTFGTYTITHDDIANALQPAFAHVVALLDTPGA
                     EFFLEARITFPTVAGAFGTADLIVRIGRTVHVIDLKFGVGVRVLALSPADDDPAVDVI
                     NGQLLFYAAAAPVSCPPIRDGTQISACRETAGGGLAKGRRKTLESSGSPTILPSARLL
                     FLPRNG"
     gene            complement(1071..1586)
                     /locus_tag="VM910_27075"
     CDS             complement(1071..1586)
                     /locus_tag="VM910_27075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66219.1"
                     /translation="MKILGVDPGIRGALAIVNSNDGATPQLVDVLDVPVIGTGAKERV
                     NTVLVWDWLAKHAPDHALIERAGSMPKQGVASTFKYARAVGSLETVIACHNIPYSIIE
                     PAVWKKFHHLRGGDKEASRQRALQLFPNARNWFARKLDHQRAEAALLALTPVSGVLPR
                     AKVTEFAEETA"
     gene            complement(1583..2563)
                     /locus_tag="VM910_27080"
     CDS             complement(1583..2563)
                     /locus_tag="VM910_27080"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HVI66220.1"
                     /translation="MNDAKLIEVGQAFLAEHPVSTVLTSDLLIDRVSLRLSAIRRHLN
                     AGGASRDLPEANRFCVGFTDRSRTDFVVRNLRLPLATSEKRQRRLRPAPLLDLLDAAP
                     PPADQHSGDVAEASPCDVSQPDENMLLMLRTAVKTAGKAAGNMSELARVLGLSSAAIA
                     QWKRIPAERIIEIERATGVRREVLRPDLYQGPTLINALAIVRAEGYRVVKPRTSKHFR
                     RPKPKDRVGPTFVCEFADGTRTRMSVFTSLENLDWGRGERLSIAAYQSRWRMRALAQY
                     HEQNGGLRLTRWQYREPAINLIAPVPPAIIAAHFEQDGKVLAQRTNGNAS"
     gene            complement(2779..3186)
                     /locus_tag="VM910_27085"
     CDS             complement(2779..3186)
                     /locus_tag="VM910_27085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66221.1"
                     /translation="MSHQPPTMSCEQITRPPQLFSISFTPRRIAMAETDWIKAAVTTL
                     DEVALAIRDLDKAELTRLCSSSRLELEFMPEIKALDRLFRERIKQLLKTTNNDDFGRF
                     FSSVCMLAAQSVLEGMLTQYKIERKLRKENRDG"
BASE COUNT          688 a         1017 c         1127 g          767 t
ORIGIN      
        1 atccacgccg tagacttacc ggcgaaggtg acctgctcgc ggcggaagcg cgcctggttg
       61 ctgatcgcgg cgccagtgcg gcgttggcac tccaggcagt ggcacatgga gatgcgcgat
      121 ggttcgccct cgatcgtaag atgaagctgc ccgcaactgc aggaggcgcg tcgggtggtc
      181 atggactggc tccgtggcgg tcgatatggc ggagtgtagg ctggactttg aaacgatgca
      241 ccatgggcag cggaatccta taaccagcta atatcggctt ctggcagatt gtgttgcaaa
      301 actattttgc ccgtcccagc ctgccggatt gacggtattg atctatgttt gctggcatca
      361 aagctcatcg ggccgcggca cattcctcag gctctggcat cacccattcc tcggcaagaa
      421 taatagacgc gcagatggca aaatcgtcgg tgagccgctg gattctaggg tcttgcgccg
      481 tcccttcgcc aaaccgcctc ccgccgtttc gcgacatgcg ctaatctggg ttccgtcgcg
      541 aatgggaggg caggaaacgg gcgccgcggc ggcgtaaaac agcagttggc cgttgataac
      601 gtcgacggcg ggatcgtcgt cggcggggct gagtgcgagc acgcgcacgc cgacgccgaa
      661 cttaagatcg atgacgtgca ccgtgcggcc gatgcgaacg attagatcgg cggtgccgaa
      721 ggcgccggcg acggttggaa aggtgatgcg ggcttcgagg aagaattcgg ccccgggcgt
      781 gtcgaggagc gcaactacgt gagcaaaggc tggttgcaag gcgttcgcga tgtcgtcgtg
      841 cgtgatcgtg taggtgccaa acgtcttgcc gacgaggtcg tcgagcgaat aggcctcgtc
      901 gagaagtagc gccatagcct catgcaacgc cgtgccgcga tcggcgtagg cggagacctt
      961 gcgcagatac gcaggcactt tctcaatgag gccaacagac gccgggcagc gcaggacgcg
     1021 cgctgcgacg ctgccgccga atggcgagtg atcgcgtcgg agctcgacgg tcatgccgtt
     1081 tcctccgcaa attcagttac ctttgctctt ggcagcactc ctgacactgg cgtgagcgcc
     1141 aggagcgcgg cttcagccct ctggtgatcg agcttgcgcg cgaaccagtt acgtgcgttg
     1201 ggaaacagct gcagcgcgcg ctgacgagag gcttctttgt cgccgccgcg cagatggtgg
     1261 aatttcttcc acacggctgg ctcgatgatg ctgtagggga tgttgtggca agcgatgacg
     1321 gtctcgaggc taccgacagc gcgggcatac ttgaaggtgg acgcgacgcc ctgctttggc
     1381 atggagccgg cgcgctcgat caacgcatga tctggggcgt gctttgcgag ccagtcccat
     1441 accaacaccg tattgactcg ctctttggcg ccagtgccga tgaccgggac gtcgagaaca
     1501 tcaacgagtt gcggcgtggc gccgtcgttg ctgttgacga tggcgagggc gccgcggatg
     1561 ccaggatcga cgccgagaat tttcatgacg cgttcccatt agtgcgttgc gctagcacct
     1621 tgccgtcctg ctcgaaatgc gccgcaatga tcgccggcgg aacgggagcg atgagattga
     1681 ttgcgggttc gcgatactgc caccgcgtca agcgcagacc gccgttttgt tcgtgatact
     1741 gagcgagcgc gcgcatccgc caccgcgact gatacgccgc tatcgacagg cgctctccgc
     1801 ggccccaatc gagattttcg agcgaggtaa acaccgacat acgcgtgcgc gtgccgtcgg
     1861 cgaattcgca cacgaaagtt gggccgacgc gatccttggg cttggggcgt ctgaagtgtt
     1921 tagaagttct cggtttgacg acgcgatagc cctcggcgcg gacgatcgcg agcgcgttga
     1981 tgagcgtcgg tccttggtag agatcggggc gcagcacttc ccgccgtacg cccgtcgcgc
     2041 gctcgatctc gataatccgc tcggctggaa tgcgcttcca ttgcgcgata gccgccgaac
     2101 tgaggccgag cacgcgtgcc agttcggaca tattgcccgc tgctttgccc gccgttttca
     2161 ccgccgtgcg caacataagc agcatgtttt cgtcgggttg cgaaacatcg cacggcgatg
     2221 cctcagccac atcgccgcta tgctgatcag ccggcggagg tgcggcgtct agcaagtcca
     2281 gtaacggcgc gggccgcagt cggcgttggc gcttctcgct ggttgcgagc ggcaatcgga
     2341 ggtttcgaac gacgaagtcg gtacgactcc gatcggtaaa cccgacgcaa aaacgattgg
     2401 cttctgggag atcgcggctg gcaccgccgg cattaagatg ccgacggatt gcgctgagcc
     2461 ggagagatac gcggtcgatc aacagatcgc ttgtaagcac ggtgctgacc ggatgctctg
     2521 cgagaaaggc ttgcccgact tcgatgagct tggcgtcgtt catgccgccc tcccggtttc
     2581 cacgaggatc agttgccgca gataatcgac ggccgctttc atgatgcatc ctcctcgatg
     2641 tcggccaacg cacgtccctc gcacccctca cagatgaatc cacgatcgat gcattgactg
     2701 cagtggatgc ccgtcacgat tggtttgccg cagatgacgc agagcaggcg cgggcgttct
     2761 aattgtcgct tctctggctc agccatcgcg gttctccttg cgaagcttgc gttcgatttt
     2821 gtattgcgtg agcatgcctt cgagcacgga ttgcgcggca agcatgcaga cggaactgaa
     2881 aaaccggccg aagtcgtcgt tgttggtagt cttcagcagc tgcttgatgc gctcccggaa
     2941 taggcgatcg agtgctttga tctccggcat aaattccaat tccagccgtg atgaactgca
     3001 caggcgggtt agctccgcct tgtcgaggtc gcggatagcc aaagcaactt catccagtgt
     3061 cgtgaccgcg gccttgatcc aatccgtttc agccattgcg attctccttg gggtaaagct
     3121 gatcgagaaa agttgcggcg gtcgggtaat ttgttcgcac gacatggttg gcggttgatg
     3181 gctcacctcc acgtcttccg actaacgatg tctctgattg ttcttggcgc gacgttatat
     3241 tgcggtccaa ggattgttga agtagcgcct atctcggctt ccttgcgaat tgcgaggacg
     3301 tcagtcagct tagctctgct attcttttca cctatatgat aaacactgaa acaagggcgg
     3361 cggcccttct cgatcatatc tcttacgttg tcccgctgag agcctaggaa caagtgagcg
     3421 ggattacagc atgagggatt atcgcaagcg tgaagcacta agcgataagt tgggatagga
     3481 ccgttggtca attcccatgc atgacgatgt gctagaatat tcctaccgtg gacgcgtagc
     3541 tgaccgtaac cctccggcag tttgcctcgg gtccaaaccc agcatttgct ttgttcgcg
//
LOCUS       DATLJT010001176         5610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2843837,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001176 DATLJT010000000
VERSION     DATLJT010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843837"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..633
                     /locus_tag="VM910_27090"
     CDS             244..633
                     /locus_tag="VM910_27090"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI66222.1"
                     /translation="MKLRYSVLLSMLVSAAVGGIAVHALHAQAKPPVYAIALNQVTDA
                     EGYAKEYLPKGRAAILAHGGVYVAAGNGTVITGDAPHDRVVVLRFESLDAVKDWFNSP
                     DYQAANQIGQKYAHYNIIAVDGVPTKQ"
     gene            complement(905..1627)
                     /locus_tag="VM910_27095"
     CDS             complement(905..1627)
                     /locus_tag="VM910_27095"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HVI66223.1"
                     /translation="MLRRPQILFAILTCAVLVTASAAQQEAADRLRMVDEIDAMLASA
                     AGNSGVARLSPRARAAMIEVPRHEFVPPEYRSSAYGNTPLPIGHGQTISQPLVVGLMT
                     ELLQLTKTDRVLEVGTGSGYQAAILSVLASEVYTIEIVPELGEMARANLERLGYSNVL
                     TKIGDGYQGWPEHAPFDAIIVTAAPNHVPPPLITQLRSGGRMVIPVGGFDQELMVLAK
                     RADGTATTTIILPVRFVPLIRE"
     gene            complement(1834..3093)
                     /locus_tag="VM910_27100"
     CDS             complement(1834..3093)
                     /locus_tag="VM910_27100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66224.1"
                     /translation="MTRADFFGSIRRAVPGLLAATIIMTGPTLAQQAGVNFPAGAGKD
                     DVVAVCGGCHDINRLKAGYTADGWRTVMRMMQNFGAPLAADHVATVTDYLIKNFPEGP
                     RPAAAVIEGPADIAIKEWPVATPGSRPHDPLAARDGSIWYTGQLANVLGRLDPRTGQI
                     KEYPVNPQTGPHGLKEDKGGNIWFTGNFSSVIGKLDPKTGKVTDYPMPDPSAKDPHSL
                     VFDREGILWFTVQQGNKIGRLDPKSGEIKLLTPPTAGARPYGIQVDSRNTIYVVEFGA
                     PKVASINPKTLAIREFPLPDPAARPRRLAIGPDDTIWYSDFARGYLGHLDPATGQVKE
                     WLSPSGPKSQPYGMVFTKGAVWYSESAAKPNTIVRFDPKTEKFQSWAIPGGGDIVRNM
                     DVTPRGEPVMANSLVNKVGLVEVKGAH"
     gene            complement(3109..3813)
                     /locus_tag="VM910_27105"
     CDS             complement(3109..3813)
                     /locus_tag="VM910_27105"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI66225.1"
                     /translation="MKYELYYWPEIQGRGEYVRLALEEAKAAYIDVAREPGGTGAMMR
                     MMEEGATQPFAPPFLKAGKLVIGQTANILFYLGARHGLAPKTEAGKLWVHQLQLTMTD
                     FVLETHDTHHPLGPSLYYEDQRVPAKKRTEEFWQERVPKYLGYFEDVLDKAGGSYLAG
                     RRLSYADLSLFQIVEGLRYAFPKRMKAFEKEIPGLTDLHDRIAQRPNIKAYLESDRRI
                     PFNESGIFRHYKELDL"
     gene            complement(3913..4574)
                     /locus_tag="VM910_27110"
                     /pseudo
     CDS             complement(3913..4574)
                     /locus_tag="VM910_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
     gene            complement(4743..>5610)
                     /locus_tag="VM910_27115"
     CDS             complement(4743..>5610)
                     /locus_tag="VM910_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HVI66226.1"
                     /translation="RIGGGFSSGSRGSRTFSAPPSTSTAPGTASPFNRTFGQPANPGM
                     NSPIGGGFFNRPGSGLLGGLAAGFLGAGLLGMLFGGGLFSGIGGFSSILGLLLQIALI
                     VFLVRFAMSWWHRRQQSEPAYAGAGGLSAGAGTGAQSNFRSGRGFGLGSGSAPLQIQP
                     ADYESFERLLVETQAAYSNEDIGTLRRLATPEMVAFFSEDLQQNRARNQINKVSGTKL
                     LQGDLAEAWREGDTDYATVAMRFSMIDRYVDRTSGRLISGSEQPTEVTEVWTFVRPRG
                     GDWMLSAIQQTS"
BASE COUNT         1012 a         1757 c         1779 g         1062 t
ORIGIN      
        1 tgattgggac ccggtccgcg gactacatca gggccagcgg tcatgaaacc gcgcaaacag
       61 gccggacaca tgacttgcac ccgaccgcga gccgaacgtc agattcctct tgcaatgcgg
      121 gcgccgtcca cacatgaccc atgtgcgaca ttgcgccggt actgcggccg tttctttggt
      181 ggacaggcta tactacggtc cttttcaccg caccgaagcg gtcaacgacg acggggcgac
      241 aacatgaaac ttcgctattc ggtactgtta tccatgctgg tcagcgcagc agttggcgga
      301 attgccgtac acgctctgca cgcccaagca aagccaccgg tttatgcgat tgcacttaat
      361 caggtcacgg atgccgaagg atatgccaag gaatacctac cgaagggtag ggctgcgatc
      421 ctcgctcatg gcggagtcta tgtcgccgct ggaaatggca ccgtcataac gggcgacgcg
      481 ccccatgatc gagtagttgt cctgagattc gaaagcttgg atgccgtgaa agactggttc
      541 aactcgccag actatcaagc ggccaaccag atcggacaga aatacgccca ctacaacatc
      601 atcgccgtag atggcgtgcc gacgaagcaa taaaggccga ggcccggaaa aagggagagg
      661 cggcctcagt tggcgtctct ccaattgaag ccgcgcaccg tgccgttttg agcctgcgag
      721 cgcccggacc tgtagctgcc gatgtccgag tctggcctgt agcagatgag gtggatggct
      781 cccgctcacg gcatcttggt gccaaggtgt gaaggccgtc aggcgttcac gagcaaaagg
      841 gagccatcca cctcatctgc taatgacccg cagcggacct gcgcaccgca gtgccgtccg
      901 ctcgctactc ccggatcaag ggcacgaacc ggacgggcaa tataatcgtg gttgtagccg
      961 ttccgtccgc gcgctttgcc aataccatga gctcttgatc gaagccgccg accgggatga
     1021 ccatgcggcc cccggatctt agttgcgtga tcagcggtgg tggaacgtgg ttaggagctg
     1081 cggtcacgat gatggcatcg aagggcgcat gctctggcca gccctggtag ccatcgccga
     1141 tctttgtcaa gacgttgctg tagccgagac gttcgaggtt ggcacgggcc atttcgccga
     1201 gctctggtac gatctcgatc gtgtacacct cgcttgcgag cacggaaagg attgccgcct
     1261 gatatccgga tccggtgccc acctccagga ccctgtccgt cttcgtcagt tgcagcagtt
     1321 ccgtcatcag cccgacgaca agcggttgcg agatcgtctg gccgtggcct atcgggagcg
     1381 gtgtgtttcc ataagcggag gacctgtatt cgggcggcac gaactcgtgt cgcggcacct
     1441 cgatcattgc ggcgcgcgca cgcgggctga gccgagccac accgctgtta cccgcggccg
     1501 acgcaagcat tgcatcgatc tcatcgacca tccgcaggcg atcggcggcc tcttgctgcg
     1561 cagcggaagc cgtcaccagc acagcacagg tcaagattgc gaataggatt tgtggcctcc
     1621 tcaacatcaa agttcgccta tcgaggagcc gcgcccaccg cggcggtcga gcagaatagc
     1681 gctccgaaac agtgccgtca ggccccgagc gtcaggacgc atgtgccgcc ggggcgttag
     1741 tcgccgcatc tccattgccc ggtcagcggg catttggggg cgaaggcctg ggcctttggc
     1801 cggcgcgggc gtcgcctgtg tgggtgcgcg cgctcagtgc gcacccttga cctcgaccag
     1861 cccgaccttg ttgactaggc tgttggccat gactggctcg ccgcggggcg tcacatccat
     1921 attgcgcacg atgtccccgc ctcccgggat cgcccagctc tggaatttct cggttttggg
     1981 atcgaagcgc acaatggtgt tcggcttggc cgccgactcg ctgtaccaga ccgcgccctt
     2041 tgtgaagacc atcccgtaag gctgcgactt cggcccgctc ggcgacagcc attccttcac
     2101 ctggcccgtc gcgggatcga gatggccgag atatccgcgc gcaaagtccg agtaccagat
     2161 cgtgtcatcc gggccgattg ccagacgccg cggccgggcg gcgggatcgg gcagcggaaa
     2221 ttcgcggatc gcaagcgttt tcggattgat cgaagcaacc ttgggcgcgc cgaactcgac
     2281 gacatagatc gtgttcctgg aatcgacctg gatgccgtag gggcgcgcgc cggccgtggg
     2341 cggcgtcagc aatttgattt cgccgctctt cggatcgagg cgcccgatct tgttgccctg
     2401 ctgcaccgta aaccagagaa tgccctcgcg gtcgaagacg agcgagtgcg gatccttggc
     2461 cgacggatcg ggcatgggat aatcggtcac cttgccggtc ttcggatcga gctttccgat
     2521 cacgctggaa aagttgccgg tgaaccagat gttgccgccc ttgtcttcct tcaggccatg
     2581 cggtccggtt tgcgggttga cgggatattc cttgatttgc ccggtccttg gatcaagccg
     2641 ccccagcacg ttggcgagct ggccggtgta ccatatcgat ccgtcgcgcg cggcaagcgg
     2701 atcgtgcggc cgtgaacccg gcgtcgcaac cggccattcc ttgatggcga tgtcggccgg
     2761 cccctcgatc acggcggctg ccgggcgcgg cccttccgga aaattcttga tcagataatc
     2821 ggtgacggtc gcgacatgat cagccgcgag cggcgcgccg aaattctgca tcatccgcat
     2881 cacggtccgc cagccgtcgg ccgtgtagcc ggccttgagg cgattgatgt cgtggcaacc
     2941 gccgcagacc gcgacgacgt cgtccttgcc cgcacccgcc ggaaaattca cgcccgcctg
     3001 ctgagccaga gtaggcccgg tcatgatgat cgtagcggcg agcaggcctg gcacggctct
     3061 gcggatcgac ccgaagaaat cagcgcgcgt catggacacc tccggacgtc aaagatcgag
     3121 ttccttgtag tggcggaaaa tgccgctctc gttgaagggg attctgcggt cgctttcgag
     3181 ataggccttg atgttcggcc gctgcgcgat ccggtcgtgc agatcggtga ggccgggaat
     3241 ttccttctcg aaggccttca tccgctttgg aaacgcatag cgcagtccct cgacgatctg
     3301 aaacagcgag agatcggcgt aactaagcct tcggccggcg agatagctgc cgccggcctt
     3361 gtcgagcacg tcttcgaaat agccgagata tttcggcacg cgctcctgcc agaactcctc
     3421 ggtgcgtttc ttggcaggca cacgctgatc ctcgtaatag agcgagggac cgagcggatg
     3481 atgcgtgtca tgagtttcca gcacgaagtc ggtcatcgtg agctgcagtt gatgcaccca
     3541 caattttccg gcttccgtct tcggcgccag cccgtggcgc gcgccgagat agaagaggat
     3601 gttcgcggtc tgcccgatca cgagcttgcc cgctttcagg aagggcggag caaacggctg
     3661 cgttgcgccc tcctccatca tccgcatcat ggcgcctgtg ccgcccggct cgcgtgcgac
     3721 gtcgatatag gcggctttgg cctcctccag cgcgagacgg acatattcgc cacggccctg
     3781 gatttccggc cagtaataaa gctcatattt catggaacgg ctcgatcctc attccgggcg
     3841 atgcgcagac accatacccg gaatccgaac gtccgggcgc taggcgtgcg catcaactcg
     3901 gaatggccag ggttagttcg ctgcaaagca gtaaagcagg ccgtcgcccc cggtgctctt
     3961 ccgggcccgc gcgagggatg cgaggagttc caggattttg aggcgtcgtc gtcgcgcaag
     4021 ccgatgcggt cggaatggcc gaccatcgcc gatccctgcg tgctgcttgt ccagttcttg
     4081 caggtccggt ccgtgtcggg cgggaacgcg gtgccatcgg gttgcgatcc cgtgagcacg
     4141 tcgtgacggt tcggcgtatc gccgcggccg ttgatcacct cgcccttttc ggagagcgaa
     4201 gtctgcttgg tgaggttgtt gttcgggccg tgcaggtcgg cgacatcttt ggcgacgacc
     4261 acgcccttgg aattctgcca gggccccttg ccgatacgat cgcgcgcatt caccgccggc
     4321 tggccgtcgg cggcctgcgt cgagagatag gcgtgccagg tcttgcctcc cgccccggct
     4381 gcctcggcca acgtctggca atgtttgtcg gcgccggcga gcccgccgag actggcgccg
     4441 ttgccggggc cggcgctggt caggaagaag gttgtgctgg ccgactgcgc ccaaaggggc
     4501 tgcgcggcga atgccgctag gcaggccgaa gcaatcaggc aagcacgcga aagatgtttt
     4561 gccgacgacg ccatttggtt cctcccattt ttttgtttgc ggtgcccgcg catgaccgcc
     4621 acggtccggt caacccggat caggatcgat tattccgatt gcgcgggcga aaagcaaagg
     4681 cgccgcggaa cgatccgcga cgcctttttt ctggcgtagg tctgttgggc cggagcggac
     4741 gatcagctcg tctgctggat cgccgagagc atccagtcac cgccacgcgg gcgcacgaag
     4801 gtccagactt ccgtgacttc ggtcggttgc tcgctgccgc tgatcagccg gccgctcgtg
     4861 cggtccacgt agcggtcgat catcgagaag cgcatcgcga cggtggcgta atcggtatcg
     4921 ccctcgcgcc aggcctcggc aaggtcgccc tgcaacaatt ttgtgccgga caccttgttg
     4981 atctggttgc gggcgcgatt ctgctggaga tcttcactga agaacgcgac catctccggc
     5041 gtcgccagcc ggcgcagcgt cccgatatcc tcgttcgaat aggccgcctg cgtttcaacc
     5101 agcagccgct cgaaggattc gtagtcggcc ggctggattt gcagcggcgc actgcccgag
     5161 cccaagccaa atccgcgacc gctgcggaag ttcgattgag cgccggtgcc ggcccccgca
     5221 ctgaggccgc ccgcacccgc ataggcaggc tccgactgtt gtcggcggtg ccaccacgac
     5281 attgcgaacc gcaccaggaa tacgatcagc gcgatctgca ggagcaggcc gagtatcgag
     5341 gaaaatccgc cgatgcctga aaacagaccg ccgccaaaca gcatgccgag caagccggcg
     5401 ccgaggaagc ctgcggcgag gccaccgagc aagccgctgc ccggccggtt gaagaagccg
     5461 ccaccgattg gcgagttcat gcccggattg gccggctggc cgaaggtacg attgaacggg
     5521 ctcgcggttc ccggcgcggt cgacgtggac ggcggcgcgg agaaagtgcg cgacccgcgc
     5581 gaacccgacg agaagccgcc gccgatgcgg
//
LOCUS       DATLJT010001177         1674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2843940,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001177 DATLJT010000000
VERSION     DATLJT010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843940"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VM910_27120"
     CDS             complement(<1..301)
                     /locus_tag="VM910_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66227.1"
                     /translation="MERLYPEIERVEVPYEGTSLPAYFVRGAGSGRRPTVVLFDGMDN
                     AKEMSVIFAGLDFAKRGINTLAIDGPGQAEALRLRNIHSRHDYEVAGTAAYDYVVF"
     gene            complement(311..691)
                     /locus_tag="VM910_27125"
     CDS             complement(311..691)
                     /locus_tag="VM910_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66228.1"
                     /translation="MTQHDRAPAEPNTWAVHFPGNLRWTNAMQIVKGMVPYAAVALDE
                     VERVGRRLKARAAEADLDRVWREEWAAMAERVAAVADTAAVEGRHITAGHHYIRAGNY
                     YYSAERFIPPGVEKMAMYRKALRA"
     gene            917..991
                     /locus_tag="VM910_27130"
     tRNA            917..991
                     /locus_tag="VM910_27130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:949..951,aa:Gly,seq:ccc)
     gene            1079..>1674
                     /locus_tag="VM910_27135"
     CDS             1079..>1674
                     /locus_tag="VM910_27135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66229.1"
                     /translation="MLPLRIVCPLAMLMILTSGASVQEVDDASFRNLAKQCQMRASVA
                     ASSRAIKIKDEAIAQHQAFNGSRVDRAGRIVSFGHSEAESDQEIDGNVSARQIPWRQV
                     LGYWEKLNDRKIGEGAGGALQVWYYPGALSDDPPPVLHRKKIQLRRLLKSISGLDLSQ
                     MGPESDDLREALQQSVIRSSISDVPWSAAFVSSIMRAVP"
BASE COUNT          315 a          499 c          523 g          337 t
ORIGIN      
        1 ggaagacgac gtagtcgtaa gcggcggtgc cggcgacctc gtagtcgtga cggctatgga
       61 tgttgcgcag gcgcagcgcc tcggcctggc cgggcccgtc gatggcgagc gtgttgatgc
      121 cgcgcttggc aaaatcgagg ccggcaaaga tcacgctcat ttccttggcg ttgtccatgc
      181 cgtcgaagag gacgacggtc ggccggcggc cgctgcccgc gccgcgcacg aaataggcgg
      241 gcaggctcgt tccctcgtag ggcacctcga cgcgctcgat ctcgggataa aggcgctcca
      301 tcgccgcgtg ctaggcgcgc agcgccttgc gatacatcgc catcttctcc acgccgggcg
      361 ggatgaagcg ctcggcgctg tagtagtagt tgcccgcgcg aatgtagtgg tggccggcgg
      421 tgatgtggcg cccttccacc gcggcggtat cggcgacggc ggcgacgcgc tcggccatcg
      481 ccgcccattc ctcgcgccag acccgatcga ggtcggcttc cgcggcgcgc gccttaagcc
      541 gccggccgac acgctcgacc tcgtccaggg caacggctgc gtagggcacc atgcccttga
      601 cgatctgcat ggcgttggtc cagcgcagat tgccgggaaa atgcaccgcc caagtatttg
      661 gctccgccgg cgcgcggtcg tgctgggtca tcgcgtggtt tcctccgtgg caaagtcctg
      721 catgtctctc gtgcaaattc ggatttggca gcatttcggt cagacgtgcg agcacgcata
      781 tgcgaccgac gctgacgtgg tcgcagatcg tcgcaagcgt aaagcacgcg cgccgcaacg
      841 ccaaagcgct tgcggtgcct gcttttcccc gtcccccttt cgcgttatcc tgcggcgccg
      901 gggctgacct ccgagcgcgg gcgtagttca gtggtagaac gacagcttcc caagctgtat
      961 gtcgagggtt cgattccctt cgcccgctcc aactctctgc gacgccatca tttctcccca
     1021 gcttccggta agactacgcc tgcaagcgtg gtcatctgcg aggccggagg aagatcgtat
     1081 gctgccgctg agaatcgttt gcccattggc aatgttgatg atcctgacct cgggggcttc
     1141 cgtgcaagaa gtcgacgatg cgtcgttcag gaatcttgcg aagcaatgtc aaatgagagc
     1201 gtcagttgca gcatcttcaa gagcaatcaa gatcaaagac gaggctatcg cgcagcatca
     1261 ggcattcaat ggaagccgtg tcgaccgggc aggacgaata gtctcttttg ggcactcgga
     1321 agcggagagc gaccaggaaa tagacggcaa tgtttctgcg agacagatcc catggcgtca
     1381 ggttttggga tattgggaaa aactcaatga caggaaaata ggggaaggcg cggggggcgc
     1441 gcttcaagtg tggtactatc ctggtgctct tagtgacgat ccgccgcctg ttctccatcg
     1501 gaaaaaaata cagctacggc gtcttttgaa atccatatca ggactcgatc tgtctcaaat
     1561 ggggcccgag tccgacgacc tacgagaggc tctgcaacaa agcgtaatcc gcagtagtat
     1621 cagcgacgtt ccgtggtcag ccgccttcgt atcttccatt atgcgcgctg ttcc
//
LOCUS       DATLJT010001178         3907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2844456,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001178 DATLJT010000000
VERSION     DATLJT010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844456"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..81
                     /locus_tag="VM910_27140"
     CDS             <1..81
                     /locus_tag="VM910_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HVI66230.1"
                     /translation="REAKDFETVLGELSGEDDARIVTIRK"
     gene            78..1226
                     /gene="ribD"
                     /locus_tag="VM910_27145"
     CDS             78..1226
                     /gene="ribD"
                     /locus_tag="VM910_27145"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVI66231.1"
                     /translation="MIFRILEDQLKQKHRASEEADHRFMQLAVTLGRRGLGRTWPNPA
                     VGAVVVKDGVIIGRGWTQPGGRPHAEPVALNRADGEARGATLYVTLEPCSHFGKSPPC
                     VDAIIAAGIARVVAAVGDPNPEVAGQGYARLRAAGIAVDTGLLAAEAARDHAGHFRRI
                     HDRRPHVILKLAVSVDDKIALAGRKPLAITGEAARTRVHLLRAQCDAILVGIGTVLAD
                     DPLLTCRLPGMEARSPVRVVLDASLRLPGDSRLLHSARKTPLWVIASELAEAQAAARL
                     GAAGAQVIRIPPKKGPGLDLPAVLHALADKGITRLLVEGGSRVASSFVAADLVDEVWL
                     LRGSNTVGPDGIAALDALPLTSITQSPIFRVRASEALEKDELTIFERA"
     gene            1226..1834
                     /locus_tag="VM910_27150"
     CDS             1226..1834
                     /locus_tag="VM910_27150"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVI66232.1"
                     /translation="MFTGIVTDIGEIVSLKAVAQGQLHRLRIGCRYDRSTIADGASIA
                     CNGICLTVVTSGVADGRTWFDVDTAAETLAMTTAKHWTVGTKLNLERALKIGDELGGH
                     IVAGHADGIATILSRDDLPDMARFELNATRDLARFIAPKGSVTLDGVSLTVNTVQDTA
                     FSVLIIPHTLNVTTLSAWRAGSEVNIEVDLMARYAARLAEMK"
     gene            1913..2404
                     /gene="ribH"
                     /locus_tag="VM910_27155"
     CDS             1913..2404
                     /gene="ribH"
                     /locus_tag="VM910_27155"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVI66233.1"
                     /translation="MADARRAPLKDQTDISGARALIVEARFYDDIQDALLEGAVAELK
                     AAGVGYDVITVPGSLEIPAAIAIALDAAQSNGRPYDAAIALGCVIRGETIHFEIVSIE
                     SSRGLLELSVNRKLPLGNGIITVNTEEQAWERARANQLNKGGDAARAALAMLRIKRRL
                     AKA"
     gene            2409..2918
                     /gene="nusB"
                     /locus_tag="VM910_27160"
     CDS             2409..2918
                     /gene="nusB"
                     /locus_tag="VM910_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVI66234.1"
                     /translation="MPESRKKPAKSQEGPKDKKANRRGAARLAAVQALYQMDIAGAGI
                     NDIFAEFESHWLGGEVEGDKYLPAEAAFFRDIVSGVVRDQARLDPLIDDALSRGWPLK
                     RIDAILRAVLRAGAYELEHRKDVPGRVVVSEYVDVAHAFVDGEETGMVNAVLDQIARQ
                     FRAEEFSRG"
     gene            2911..3906
                     /gene="thiL"
                     /locus_tag="VM910_27165"
     CDS             2911..3906
                     /gene="thiL"
                     /locus_tag="VM910_27165"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HVI66235.1"
                     /translation="MADDHSGEHALIARYFKPLATDPGAFGLDDDAAVLEAPHGEEIV
                     VTTDAIVEGVHFLADDPPDTVARKALRVNLSDLAAKGAQPAGFVLTLALRGADDAWLT
                     AFARGLGEDAKLFDCPLLGGDTVSTPGPLTVSISAFGRVPAGKMVHRTGARPGDRVMV
                     SGTIGDAALGLHVLKGDRIAEALAGDEAGRAMLVARYRVPQPRDALAAAVREFANAAM
                     DVSDGLAGDLAKLCAASGVSALIDAPSIPKSAAAAYLLAQGVAGIETLVSGGDDYEIL
                     CTVTEARLEAFAAEARRAGVALTSIGTVIAGAHAPRFLDEQGSEIVLKRLSFSHF"
BASE COUNT          688 a         1216 c         1302 g          701 t
ORIGIN      
        1 cgcgaggcca aggacttcga gacagtgctc ggcgagctct ccggcgagga cgacgcgaga
       61 atcgtcacga tacgaaaatg atcttccgca tcctggagga tcagcttaag cagaaacata
      121 gggcgtccga ggaagccgat catcgcttca tgcagcttgc tgtcacgctc ggccggcgcg
      181 gactcggacg cacctggccg aacccggccg tcggcgcggt cgtggtcaaa gacggcgtca
      241 ttatcgggcg gggctggacc cagccgggcg gccgtccgca tgcagagccg gtggcgctga
      301 accgcgctga tggtgaggcg cggggcgcca cgctctatgt gacgctggag ccatgctcgc
      361 atttcggaaa gtcgccgcct tgcgtcgacg cgatcattgc cgccgggatt gcgcgggtgg
      421 tggctgcggt cggggacccc aaccccgaag tcgcgggtca gggctacgcc agattgcgcg
      481 ccgcagggat cgcagtcgac accggtcttt tggcggcgga agctgcccgc gaccacgccg
      541 gtcattttcg ccgcatccac gatcgccgcc cgcatgtgat cttaaagctt gcggtgtccg
      601 tggacgacaa gattgctttg gccggccgca agccgttggc gatcacgggt gaggccgcgc
      661 ggaccagggt acatctgttg cgggcgcaat gtgacgcgat cctggtcggg atcgggaccg
      721 tgctcgccga cgatccgctt ttgacctgcc gcctgccggg catggaggcg cgctcgccgg
      781 tgcgggtggt gctggacgcg tcgctgcgcc tgcccggcga cagccgcttg ctccattccg
      841 cgcggaagac gccgctctgg gtcatcgcat ccgaattggc ggaggcgcag gctgcggcgc
      901 ggcttggcgc ggcgggtgcg caggtgatcc gcattccgcc gaaaaagggg cccgggctcg
      961 atcttccggc cgtcctgcat gcccttgcgg acaagggtat cacgcggctg ttggttgagg
     1021 gcggcagcag ggtcgcatcg tcctttgttg ccgccgacct cgttgacgag gtctggctgc
     1081 tgcgtgggtc gaacacagtc ggaccagatg gcatcgctgc actggatgca ttgccgctca
     1141 cttcaatcac gcagtcgccc attttccgcg ttcgtgctag cgaagcgctg gaaaaagacg
     1201 aactgaccat tttcgagcgc gcctgatgtt caccggcata gtcaccgata tcggcgagat
     1261 cgtttcgctc aaggccgtcg cgcaaggcca gttgcaccga ctgcgtatcg gctgccgcta
     1321 cgatcgctcc acgattgccg acggtgcctc aatcgcctgc aacggcattt gcctcacggt
     1381 tgtcacgtca ggtgttgccg atggcaggac ctggttcgat gtcgatactg cggccgaaac
     1441 gctggccatg actaccgcga agcattggac cgtcggcaca aaactcaatc tcgagcgcgc
     1501 gctcaagatc ggcgacgaat tgggcgggca tatcgtggcg ggccacgccg acggcatcgc
     1561 caccatacta agccgcgacg atcttcccga catggcgcgg ttcgaactca atgccacgcg
     1621 cgatctcgcg cgcttcatcg cgccaaaagg ttcggtgacg ctggatgggg tctcccttac
     1681 cgtgaatacg gtgcaagaca cggcattctc cgtgctgatt attccgcata cactcaacgt
     1741 gacgacgctg tcagcctggc gtgcgggcag cgaggtcaat atcgaggtcg acctgatggc
     1801 gcgctacgcg gcgcggcttg cggaaatgaa atagaggcag tcggctttgt cccttggctt
     1861 tgcctgaagt gacgactaca aggctcacgc ggaacgaaat ccggaattga ccatggcaga
     1921 tgcgcggcgc gccccgttaa aagaccagac cgacatttcc ggcgcacgcg cgctgatcgt
     1981 cgaagcgcgc ttctacgacg acatccagga cgcgctgttg gaaggggcgg tcgccgaact
     2041 gaaggccgcc ggcgtcggct atgacgtgat cacggttccc ggttcgctgg agattccggc
     2101 cgcgatcgcg atcgcgctcg atgccgccca aagcaatggc agaccctatg atgccgcgat
     2161 cgcgcttggc tgtgtcatca ggggtgaaac catccatttc gagatcgtct cgatcgagtc
     2221 ctcgcgcggc ctgttggagc tttcggtgaa cagaaaattg ccgctcggca acggcatcat
     2281 caccgtcaac accgaggagc aggcctggga gcgggcgcgc gcgaaccaac ttaacaaggg
     2341 cggcgatgcg gcacgcgcgg cgctcgccat gctgcggatc aagcgacggt tggcgaaagc
     2401 gtgaggccat gcctgagagc aggaaaaagc cggccaaaag ccaggaaggg cccaaagaca
     2461 agaaagccaa ccgtcgcggg gccgcgcggc tcgccgccgt gcaggcgctc tatcagatgg
     2521 acattgccgg tgccgggatt aacgacatct tcgctgaatt cgaaagtcac tggcttggag
     2581 gcgaggtcga gggcgataaa tatctccccg ccgaagccgc attcttccgg gacatcgtgt
     2641 ctggcgtggt gcgcgaccag gcaaggctcg atcctctcat cgacgacgcc ctgtccagag
     2701 gctggccgtt gaagcggatc gacgcgatcc tgcgcgcggt gctgcgcgcc ggagcctacg
     2761 agctcgagca tcgcaaggac gtgcccggcc gcgtcgtggt ctccgaatat gtcgacgtgg
     2821 ctcatgcctt cgtcgatggc gaggagaccg gcatggtgaa cgcggtgctg gatcagattg
     2881 cacggcaatt ccgcgccgaa gagttttccc gtggctgacg atcactcagg cgaacacgct
     2941 ctgatcgcgc gctatttcaa gccgcttgcg accgatcccg gcgcgttcgg tctcgacgac
     3001 gatgccgcgg tgctcgaagc cccccacggc gaagagatcg tggtgacgac cgatgcaatc
     3061 gtcgaaggcg tgcatttttt ggccgacgac ccgcccgata cggtcgcgcg aaaggcgctg
     3121 cgggtgaacc tgtccgatct cgctgccaag ggtgcgcagc cggcaggctt cgtcttgacg
     3181 ctggcgttgc gcggcgcgga cgatgcctgg ctcaccgcgt tcgcccgcgg gcttggcgaa
     3241 gacgcaaaat tgttcgactg cccgctgctc ggcggcgaca ccgtgtcgac cccgggtccg
     3301 ctgacagtct cgatatcggc gttcgggcgc gtgccggcgg gcaagatggt ccatcgcact
     3361 ggtgccaggc ccggcgaccg cgtgatggta tcgggcacga ttggtgatgc ggcattggga
     3421 ctgcacgtct tgaagggaga tcgcatcgcc gaggcgctcg ccggtgacga ggcgggcagg
     3481 gcgatgctgg ttgcccgcta ccgcgttccg cagccgcgcg atgcactcgc tgcggcagtg
     3541 cgcgaatttg ccaacgcggc gatggatgtc tccgacggac ttgccggcga tctcgcaaag
     3601 ctctgcgccg catcgggcgt ctctgcgctg atcgacgcac ccagcatccc gaaatcggct
     3661 gccgcggctt acctgctcgc ccaaggtgtt gccggcatcg agacgctggt ctcgggcggc
     3721 gacgactacg agattctgtg caccgtcacc gaggcacgtc tcgaggcttt cgctgcggaa
     3781 gcacgacgtg cgggcgtcgc gctaacctcg atcggaactg tgattgcggg cgcccacgcc
     3841 ccaagatttc tggacgagca gggcagcgag atcgtgctca agcgcctgtc cttcagccat
     3901 ttctaga
//
LOCUS       DATLJT010001179         1573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_86013,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001179 DATLJT010000000
VERSION     DATLJT010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86013"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(702..950)
                     /locus_tag="VM910_27170"
     CDS             complement(702..950)
                     /locus_tag="VM910_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66236.1"
                     /translation="MAFKPNYRRDRAERQRAARARTEEKQRKKEEKAALRKAEREATE
                     APPDEKQTISIAMPFNASTRATITLCNGLRRIDCRHLG"
BASE COUNT          283 a          471 c          471 g          348 t
ORIGIN      
        1 agtgggaggt acggttgccg atcccgggtc atctgctgtc ggataagcat cccaccttgc
       61 ataagaccaa agaagcagcg gacctttgga tcgcaagtcc cgacggccgc aagtgggaag
      121 aacagaaact cgctaaatat aagaagagct gatcggccct acccttgtgg cttctgtggc
      181 ggcagcttat gcgccaactc gggccattcg ttttcgacca cagcgcgcat ttcatccgag
      241 atatggtcga tgtgctctat atcgagcatg tacttgtgag gcgcttcagg tccgccggca
      301 gcatgacctt gccgccgacc aggcggggct tatagacgcg ccgctctctg gtcattgccg
      361 cctactgtca gccggattgc ggtcggccgg catatagatg ttccagcgcg cttgcgcacc
      421 agcgctcgcc cgggtggcgc gcataagtaa gtttgcttac ccgccttcgc ggggaagcca
      481 tgcgcggtcg cgggcggtca cgaatcgcgg cacgattcgg cgctctcaat aagcttttcg
      541 aacgccttga tgagtgcttc ccacatgttc ggcccccaag tgagaacatg aaggcgcgcg
      601 tgcggaaaaa cgctcggcgt caaggaagtg tcacttcagt agatcgggac caatccaagc
      661 gtcggttgcg ggccgctatc tatgtgaccg cggctgcaac gtcaacccag atgtcggcaa
      721 tcaatccggc gcaggccgtt gcaaagggta atcgtcgctc tcgtagaggc gttgaatggc
      781 atcgctatcg aaatcgtttg cttctcatcc ggcggggctt cggttgcctc gcgctcggct
      841 ttgcgcagcg cagccttctc ctcctttttt ctttgttttt cctcggtgcg tgcgcgtgct
      901 gccctctgtc gctcggcccg gtctcgacgg taattcggct tgaatgccat tggctccctc
      961 ctttgcggcg ggttgattta gtgcgcggcg accctgtaca tgcacgcgtt cgagttttgc
     1021 ccgtccttgc cattacccgc tcaggctagg tggtggggga atttggtctt gccggccgtg
     1081 gctcgatgat cacgatgccg cgccaccgag gatgcctccc tggtcggtga ctcctgttcc
     1141 cggagtcttg cctcaatttt aggccagcta tgcaaaaaag tcgcgccctc tttttgccgt
     1201 cggtcgagat gtcgcgcgga attgtccagc ggctcgtccg acccacgctc gctgcaccgc
     1261 atgcccgccg gaggtcgcag gccggcgcaa atctatgatt tcaagcgcga aaccgggtgg
     1321 cgggatcgcg caccgagcgt ggaaagctcg tgtctgagct cagtccagag aatgtctgct
     1381 gactctgaag tcaggcacca tagctgccgt ggtccggtcc atgcaccacc cggcgggcct
     1441 cctggaatcg atggaaattc ggtggtgggg atagcgtacg gagccaaacg tccacccagt
     1501 gtgtctcaag tgccgactca acgccaacca cattgacggt gaaggtatca gcaccgcgag
     1561 ccaggcacca tgg
//
LOCUS       DATLJT010001180         3763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4221699,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001180 DATLJT010000000
VERSION     DATLJT010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221699"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VM910_27175"
     CDS             complement(<1..575)
                     /locus_tag="VM910_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66237.1"
                     /translation="MQFGFLLEQVVNGLVLGGYYLLIALGLSLIFSVGGIVNLAHGAF
                     YALGAYASVEITKYLGFGPAVVISPVAVAILGIVFERFILRRFYTADPILSLLVTFAL
                     AMVTEQAIRIIWGAAPLSASIPPAFRGSVFVGDFLFSRYRLLILAVVAAVLLGVWLLL
                     HKTSFGRVVRAGIQRPDMVASLGIRLEPYMTA"
     gene            complement(767..2008)
                     /locus_tag="VM910_27180"
     CDS             complement(767..2008)
                     /locus_tag="VM910_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66238.1"
                     /translation="MTTRFSRRTLIKAGAAFTGASTIGFPAIVGAQADKIRIGHLTPL
                     TGFLGALGAYAQLGIRMAAEEINAAGGVAGRPLDILSEDSVNPATAATKAQRMLDQDG
                     VAFLMGEINSASALTIMQVAERNKRLFMQIGARSDALRGKNCNRYTFHVDIPSTVMVN
                     AVGKALVRENMMKDKKFYTLTADYIFGHDLARAAKAFFDANGGKLIGDELVATDVTDF
                     SPYLLKIRQSQPDVVCSNLAGNQVTNLIKQYAEFGLPYPIVGFNLNTADAWAAGDGNL
                     SGTWPTVWYHTLDVPASKTFVEGFIKKYGKPPENHAWIEYASLKIVAQAINETKSTET
                     DKLIAYLEKETQFDILKGRKGYFRSWDHQLMQEAYPFTVKAKGKAKDQWETLELGAAI
                     PGANEPLESIALTKEQNPCSM"
     gene            complement(2160..3059)
                     /locus_tag="VM910_27185"
     CDS             complement(2160..3059)
                     /locus_tag="VM910_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVI66239.1"
                     /translation="MRSRPRQKISEKPANLRKATVAKLVQPVQATTDIDDEAEDRQRA
                     GVQSLGRAFAILEEVARHREGIGLAELSKLVSLHNSTTFHLAKTLVSLGYLRQEKESK
                     RYRVGRPLFALAASALDEIEMVNVATPILEELSRQTGESSHFAVRMGDTVVVIARTSG
                     PGAFQLTDRVGVVRPAHCTALGKIILASLRPDQLERFLERVELKPSTPKSITEIPALM
                     REIAEIKRTGIAFDDGEFNPEVRCVAVPVTDFTGQVVGALGISGPIWRLSNQALHASA
                     QTVQAAAGRLSAEFGATRTAKRA"
     gene            3264..>3763
                     /locus_tag="VM910_27190"
     CDS             3264..>3763
                     /locus_tag="VM910_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI66240.1"
                     /translation="MSGNRLNGAQVIVDYLIQEKVPQVFGLCGHGNIQFIDALYERSS
                     EIKTISVHHESVAGFMADVYYRVSGRPTATFTSCGPGSANLPISLANAFLDSVPFMAV
                     TGNVPTSQFNRGAFQEMYRHYQADFPSTVRSICKKVFQPTRGEMVPLAVRQAWKTMIT
                     GRPGPVV"
BASE COUNT          712 a         1102 c         1190 g          759 t
ORIGIN      
        1 gccgtcatat aaggctccag gcggatgccg agcgaggcga ccatgtcggg tcgttgaatg
       61 ccggcgcgca ccacgcggcc gaacgaagtc ttgtgcaaga gcagccagac gccgagcagc
      121 accgcggcga ccacggcgag gatcaggagg cgatagcgcg aaaacaggaa gtcgccgaca
      181 aagaccgagc cgcggaaggc gggtgggatc gacgcggaga ggggcgcggc accccagatg
      241 atgcggatcg cctgttctgt gaccatggcg agcgcgaagg tcacgagcag gctcaggatc
      301 ggatcggcgg tatagaagcg gcgcaggatg aagcgctcga agacgatgcc gagaattgcg
      361 acggcgaccg gcgagatcac cacggccggg ccaaaaccga gatatttggt gatctcgaca
      421 gaggcatagg cgccgagcgc gtagaacgcg ccgtgcgcga gattgacgat gccgcccacg
      481 ctgaaaatca gcgacagccc gagtgcgatc agaagataat agcctccgag cacgaggcca
      541 ttcaccacct gttcgagcag aaatccgaac tgcatggcta gtggtccgat tctaacattc
      601 gcactcgatt ttggcaggct cgttagcgaa tgctggaatc aagggaccac tggcaagcct
      661 gggctcatcg tggacctctg ggtttgacat tcgcctgcga actcgcggga ggcgcggagc
      721 gaatgtcaaa tccgctccac ggtatccaga agagcgacag cctcgattac atcgagcagg
      781 gattctgttc cttggtgagc gcgatcgatt ccagcggttc gtttgcaccc gggatcgccg
      841 cgcccagttc cagcgtctcc cattggtcct tcgccttgcc cttggccttg acggtgaagg
      901 gatatgcttc ctgcatgagc tggtgatccc agcttcggaa atagcccttg cgtcctttga
      961 ggatgtcgaa ctgcgtttcc ttttcgagat aggcgatcag cttgtcggtt tcggtcgatt
     1021 tggtctcgtt gatcgcctgc gccacgatct tgagcgaggc atattcgatc caggcatggt
     1081 tctccggcgg cttgccgtac ttttttatga agccctcgac gaacgtcttt gaggccggca
     1141 cgtcgagcgt gtggtaccag acggtcggcc aggtgccgct cagattgcca tcgccggccg
     1201 cccaggcatc cgcagtgttg agattgaagc cgacgatcgg gtagggcagg ccgaactcgg
     1261 catattgctt gatcaggttg gtgacctgat tgccggcaag gttggagcag accacgtcgg
     1321 gctgcgactg gcggattttc aggagatacg ggctgaagtc ggtgacgtcg gttgcgacca
     1381 gctcgtcgcc gatcagcttg ccgccattgg cgtcgaagaa cgctttggcc gcgcgcgcaa
     1441 gatcgtggcc gaaaatgtag tcggcggtca gcgtgtagaa cttcttgtcc ttcatcatgt
     1501 tctcgcggac cagcgccttg ccgaccgcat tcaccatcac ggtgctcgga atgtcgacat
     1561 ggaaggtgta cctgttgcag ttcttgccgc gcagcgcgtc ggagcgggcg ccgatctgca
     1621 tgaacaaacg cttgttgcgc tcggcgacct gcatgatggt cagcgccgag gccgaattga
     1681 tttcgcccat caaaaacgcg acgccatcct ggtcgagcat gcgttgcgcc ttggttgcgg
     1741 cggtggcggg attgacggaa tcctccgaga ggatgtcgag cggccgcccg gcgacgccgc
     1801 cggccgcatt gatttcttcc gccgccatgc gaatgccgag ttgcgcatag gcgccgagcg
     1861 cgcccaaaaa tccggtaagc ggcgtgagat gaccgatcct gattttgtca gcctgcgcac
     1921 cgacgatcgc aggaaagccg atggtcgaag cgccggtaaa tgcggcgccg gccttgatca
     1981 atgtgcgtcg gctgaagcgg gtcgtcatgg ctgatctctc tcccttgtgg ctctgtgcgc
     2041 gctgcctccc ttccggaccg ctatgcgagg atgtgagaga gggttttcta ttgtcggata
     2101 atatcggcgg tcgtggcccg cactgtcaac cggcaaaaaa cacggccttt gttttcgccc
     2161 tacgcccgct ttgcggtgcg cgttgcgccg aactcggcgg acaggcgccc tgctgcggct
     2221 tgaacggtct gtgcgctcgc gtgcagggcc tgattggaga gccgccagat cggaccggaa
     2281 atgccaagcg cgcccacgac ctgtcccgtg aaatcggtga ccggcaccgc cacgcagcgc
     2341 acttccggat tgaattcacc gtcgtcgaag gcaatgccgg tgcgcttgat ctcggcaatc
     2401 tcccgcatca atgccgggat ttcggtgatc gatttgggcg ttgagggttt cagctcgacg
     2461 cgctcgagga agcgttcgag ttgatcggga cggagcgaag cgaggatgat cttgccgagc
     2521 gcagtgcagt gagcggggcg aaccacgccg acgcggtcgg tgagctgaaa ggcgccgggc
     2581 ccgctggtgc gcgcgatcac gacgacggta tcgcccattc gcaccgcaaa atggctgctc
     2641 tcgccggttt gtcgcgacaa ctcctccaga atgggtgttg cgacattgac catttcgatt
     2701 tcatcgagcg cgcttgccgc aagcgcaaac agcggcctgc cgacgcgata gcgtttggat
     2761 tctttctcct ggcgcagata gccgagcgag accagcgttt tcgccagatg aaaggtggtg
     2821 gaattgtgca ggctcacgag cttgctcaat tcggcaaggc caatgccctc gcgatgccgc
     2881 gcgacctctt cgagaatcgc aaaagcacgt ccgagcgact gcacgcccgc acgctgccgg
     2941 tcctccgcct cgtcgtcgat gtcggttgtc gcctggaccg gctgcaccag tttcgcgacc
     3001 gtcgctttgc gcaggtttgc cggcttttcg ctgatctttt gtctgggtcg tgatctcacc
     3061 ggattaggcc tccacgcgac gcatgagccg aacgctcttt cctgtttctg gccgctgcta
     3121 tgattcccta caaactgagg caatctatca caatattaat tgacgtacag cattatgaga
     3181 aatagggtgc cgcgcgcccc ggggcagcag ccggcgtatg cctctggctt tcgccatagt
     3241 cgaacgaacg aggagagggt ccgatgtcag gaaacaggct caacggtgcc caggtcatcg
     3301 tcgactatct gatccaggag aaggtgccgc aggtgttcgg cctgtgcggc cacggcaata
     3361 tccagttcat cgacgcgctc tacgagcgtt cgtcggaaat caagaccatt tcggtgcatc
     3421 acgagagcgt cgcgggattc atggccgacg tttactatcg cgtttcggga cggccgaccg
     3481 cgacgtttac ctcctgcggc cccggctcgg cgaacctgcc gatctcgctt gcgaacgcct
     3541 ttctcgattc ggtgccgttc atggcagtga ccggcaatgt gccgaccagc caattcaacc
     3601 gcggcgcatt ccaggaaatg taccgtcact atcaggctga ctttccctcc accgtgcgct
     3661 cgatctgcaa gaaagtgttt cagccgacgc gcggcgagat ggtgccgctc gcggttcggc
     3721 aagcctggaa gaccatgatc acggggcgtc cggggccggt ggt
//
LOCUS       DATLJT010001181         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3763430,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001181 DATLJT010000000
VERSION     DATLJT010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763430"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VM910_27195"
     CDS             complement(<1..240)
                     /locus_tag="VM910_27195"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HVI66241.1"
                     /translation="MTPEQKRLVRDTWKQVAPVADAAAGMFYHRLFEIDPTTRKLFRA
                     TDMAAQRKKLLQTLAFAINGLDNLGALVTKVEGLGR"
     gene            complement(289..489)
                     /locus_tag="VM910_27200"
     CDS             complement(289..489)
                     /locus_tag="VM910_27200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66242.1"
                     /translation="MRNVTRIVTAVLTAVTLSLLAAMPLVAGRRTPAEPSAASVSVNP
                     LELMRQAGDLPGQIIQDLMTVY"
     gene            810..1352
                     /locus_tag="VM910_27205"
     CDS             810..1352
                     /locus_tag="VM910_27205"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HVI66243.1"
                     /translation="MWANYLMKLVDRVKAILLTPQAEWKVIEQERDTLFDLLISYVAI
                     LAAIPEIAHFIGQSLIGGYAPIVPSLVRAVVVYLVAFAMVYIIAGVIDLLAPRFGVEK
                     NFPNAVKLSAYSHTPLWLAGIFLLIPGLNFLLILGLYGFYLLWVGLPMLMKVPNDKAL
                     PYAAMVTACALIPAIVLTMI"
     gene            1426..1779
                     /locus_tag="VM910_27210"
     CDS             1426..1779
                     /locus_tag="VM910_27210"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HVI66244.1"
                     /translation="MAWLIRKSAALIAVYAIALQALIAGFVVAGHSGFDPLAIICTAD
                     GSGDHQPAPTQHRSDCDACLAACSSSPALVPANVAFSPVLFPATPKRPTVFAEPVSLQ
                     PRHQPHESRAPPMQS"
     gene            1870..2361
                     /locus_tag="VM910_27215"
     CDS             1870..2361
                     /locus_tag="VM910_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HVI66245.1"
                     /translation="MRPIVFVFAAAFGLAVTAASAGDYKAGSLDISNPWSRATPKGSS
                     VAAGYVKITNSGSAPDRLIGGSSDVASKFEVHEMRMENGVAKMRPVTGGLEIKPGETV
                     ELKPGSFHIMFVGLKKPLSAGDQFKATLVFEKAGTVNVDYDVRAMGSEGGGGMPGMQM
                     HGH"
BASE COUNT          406 a          737 c          754 g          464 t
ORIGIN      
        1 gcggccgaga ccttcgacct tcgttaccag cgcgccgaga ttgtccaggc cattgatggc
       61 gaaggccaac gtctgcaaca gctttttgcg ttgcgcggcc atatcggtcg cgcggaatag
      121 cttgcgcgtg gtggggtcga tttcgaacag ccgatggtag aacatgccgg ctgccgcgtc
      181 ggcgacggga gccacctgtt tccaggtgtc ccgcacgagt cgcttctgtt cgggggtcat
      241 cgcgttgctc tatgcttgcg ccgccgcggc ctttgccgcg tcgcttcctc agtagaccgt
      301 catcaggtcc tggatgatct ggcccggcag atcgccggcc tgccgcatca gctcgagcgg
      361 gttgaccgac accgacgccg cggatggctc ggcgggcgtg cgccggccag cgaccagcgg
      421 catcgccgcc aacaggctca aggtgaccgc ggtcagcaca gcggtgacga tacgggtaac
      481 gttgcgcata ggaacctcca tcaagccgcc ggcaggtgcc gggagcattt ggaggtttgc
      541 ttagccgcag acgcgatcag ccgcttgagg cggagttgaa gttgatgctg aatctgcttg
      601 aagattttct ggagattttc tgaagatggt ataatggcgc tcccaggcgg gctgccgctc
      661 ggcggttccg agggtaacct ttgggcgacg gtcacggacg ccgtcgcaag cccaggtcgt
      721 caagcgatcc ccatgcaccg acgatgaaaa tcgcgcacaa ggcatgatcc ggaagggctg
      781 gcgcgcgcag atgccgcttg gagggccata tgtgggccaa ttacctgatg aagctggtcg
      841 atcgggtcaa ggcgatcctg ctgacgcccc aggcggaatg gaaggtgatc gagcaggagc
      901 gggacacgct cttcgatctc ttgatcagct acgtcgcgat cctcgccgcg atccctgaga
      961 tcgcccactt catcggccag tcgctgatcg gcggctacgc gccgatcgtt cccagtctcg
     1021 tgcgcgcggt cgtcgtctac ctcgtggcct tcgcgatggt ctacatcatc gcgggggtga
     1081 tcgatctgct ggcgccgaga ttcggcgtcg agaagaattt tcccaacgcc gtcaagctgt
     1141 cggcctattc ccacacgccg ctctggcttg ccggtatttt tctgctcatc ccgggactga
     1201 acttcctgtt gatcctgggc ctttacgggt tctacctgct gtgggtcggg ctgcccatgc
     1261 tgatgaaggt gcccaacgac aaggcgctgc cctacgcggc gatggttacc gcttgcgcgc
     1321 tcatccccgc gatcgtgctc acgatgatct agccaacgca cggccgaaat cccgcctcaa
     1381 ccgaagctgg acgcgtcgca ggacgcggcg gtatgatggc tcgccatggc gtggttgatc
     1441 cgaaaatccg cggcgctgat tgcggtctat gcgatcgcgc tgcaggcctt gatcgcgggc
     1501 ttcgtggtcg cgggccattc ggggtttgac ccgctcgcga tcatctgcac ggccgatggt
     1561 tcgggagacc accagcctgc gcccacgcaa caccgcagcg actgcgatgc ctgtctcgcc
     1621 gcatgcagca gttcaccggc tctcgttccc gcaaacgtag cgttctcgcc ggtactcttt
     1681 cctgcgacgc cgaagcgtcc gacggttttt gccgaaccgg tttcattgca accgcggcat
     1741 cagccgcacg aatcgcgcgc acctccgatg caatcctgac ctgagcgcct gccggcgctc
     1801 acctgcatgc atgtcggccg caccgtagag cccgcggccg gtcattcaac attgctcagg
     1861 atggtagaaa tgcgccccat cgttttcgtc tttgccgcgg ccttcggctt ggctgtcacc
     1921 gccgcgagcg ccggcgacta caaggccggc tcgctcgaca tttccaatcc atggtcgcgc
     1981 gccaccccga agggatcgag cgttgccgcc ggctacgtga agatcacgaa cagcggatcg
     2041 gcgcccgatc ggctgatcgg cggatcgtcg gacgtcgcat ccaaattcga agttcacgag
     2101 atgcggatgg agaacggcgt ggcgaagatg cggccggtca ccgggggatt ggaaatcaag
     2161 ccaggggaga ccgtcgagct caagcctggc tcgtttcaca tcatgttcgt tggtttgaag
     2221 aaaccgctca gtgccgggga ccagttcaag gcgacgctgg tgttcgaaaa ggccggcacc
     2281 gtgaacgtcg actatgacgt gcgcgcgatg ggctcggaag gcggcggcgg catgcccggc
     2341 atgcagatgc acgggcactg a
//
LOCUS       DATLJT010001182         2924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4221968,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001182 DATLJT010000000
VERSION     DATLJT010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4221968"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1407)
                     /locus_tag="VM910_27220"
     CDS             complement(232..1407)
                     /locus_tag="VM910_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI66246.1"
                     /translation="MQPKHALLSSFSGATFLAIASLNSVQAAPLLIVGNDEKLLWDDQ
                     GKPIVSPAGKDSVVILDLADPESPKIVVNLPLKNSIVGPPVNLAIDPTNSIALVADSI
                     DVTKDGDALKQGPDNKVYVIDLKANPPKLADTVTVGKQPSGLSFNPAGNLALVTNRAD
                     KSISVLSVKGTDVKVIDTVDMGDIVSHVEFTPDGKRALATKFNAHKVSLLDINGDKVT
                     YTKLDLPTGQWPYNVAVAPSGKIALTADNGGAGSSDGSVDTVSVVDLELNPIRIIDRV
                     VVGDGPEGLAISPKGDVAVAVILAGSNNKSAYFYKRNGHIAVLRIDGKKVTKVGDVEA
                     GGLPEGAAFTPDGKYLFVGNYLDQDLSILKVNGAEISDTGKRFKLPGHPASVRMSTH"
     gene            complement(1667..2869)
                     /locus_tag="VM910_27225"
     CDS             complement(1667..2869)
                     /locus_tag="VM910_27225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVI66247.1"
                     /translation="MTKSSLALSNLRGFAIVMVVAFHSCIAYLNSQPDAALPFDIAPY
                     GWRANPIVDSARWLGFDLFCAFQYIYLMQLLFFLSGLFVWPSLSRKGGGKFLYDRFLR
                     LGVPFAIGLYVLMPVAYYPVYRVTAVDPSWSAFWSHWIALPFWPSGPMWFLWLLLVFN
                     LAAAGLFWLAPRAGEALGELSAKAGSHPGRFFIALVSVLALAYLPLAAVFEPWQWMQF
                     GPFAFQPSLAPQYAVYFFAGLGVGAYGFDRGLLEGDGVLARRWARWLVGTAAAFLLWV
                     VPTALIVHGTLVPGLRLVASLGLVLCPASACLALAAVFLRFAGRQRPVFESLADNAYG
                     IYFVHYVFVIWLQYMLLHAPLFAVAKAAIVFTGTLTLSWAITAVICRIPIGARLMGRN
                     RRELARAR"
BASE COUNT          614 a          905 c          892 g          513 t
ORIGIN      
        1 gtgaacgtgt cgcaactcgc gccatcaatc gacgtcgggc tcgccgcatg gagcaccaaa
       61 tccgtaccaa gcgaaaatac acaaccaaaa aactgcggtg ggtgaaatag atgcgcgcgc
      121 tcacacgctc ccttggtaag ggggaggtcg caagttcaat ccttaccggc agcgccacaa
      181 gttcaggaaa actaaagcct gcaaaccgga tatcacgcgc ccccgtagca cctagtgcgt
      241 gctcatccgg actgaagcgg gatggccggg cagtttgaac cgtttcccgg tgtcgctgat
      301 ctcggcgccg ttgaccttga ggatggagag atcctggtcg aggtaattgc cgacaaacag
      361 gtactttccg tcgggcgtaa atgccgctcc ttccggtaaa ccgcccgctt cgacatcgcc
      421 gactttggtg actttcttgc cgtcgatgcg cagcactgcg atgtggccat tgcgcttgta
      481 aaaataggcg ctcttgttgt tggaaccggc caggatcacc gcgaccgcca catcgccctt
      541 cggcgagatg gcgagccctt ccggcccatc gccgaccaca acgcggtcga taatgcggat
      601 gggattgagt tcgaggtcga ccacgctcac cgtatcgacg ctgccgtccg aggagccggc
      661 gccaccgttg tccgccgtga gggcaatctt gccgcttggc gccaccgcga cattgtaggg
      721 ccactgcccg gtcggcaggt caagcttggt ataggtcacc ttgtcgccgt tgatgtcgag
      781 cagcgaaacc ttgtgagcgt tgaatttggt ggcgagcgcg cgcttgccgt cgggcgtaaa
      841 ctcgacgtgc gaaacgatgt cgcccatgtc gaccgtgtcg atgactttca cgtcggtgcc
      901 cttcaccgac aagacgctga tcgacttgtc ggcacggttg gtgacgagcg cgaggttacc
      961 tgccggattg aagctgaggc ccgatggttg cttgccaacc gtcaccgtgt cggcgagctt
     1021 gggcgggttg gctttcagat cgatgacgta aactttgttg tcggggccct gctttaacgc
     1081 gtcaccgtct ttggtgacgt cgatcgaatc ggcaaccagt gcgatcgagt tggtgggatc
     1141 gatagcgagg ttgaccggcg gaccgacgat cgagttcttc agcggcaggt tcacgacgat
     1201 cttcgggctt tccggatcgg cgagatcgag gatcaccacc gaatctttgc cggccggcga
     1261 cacgatcggc ttaccttggt catcccataa cagcttctcg tcgttgccga cgatcagcaa
     1321 tggtgcggcc tggacggaat tcaaactggc gatagccaga aacgtcgcgc cgctgaacga
     1381 cgaaagcaac gcatgttttg gttgcatgtg ttttcctccc tcttgccagc cgacatttcc
     1441 tcccgatcct caacacatga tcatcgacct ttctgcacgc gtgggcaatc caggttttga
     1501 cgcgataagc tttgtcgcca aggcttccgg aagcgcgact tgcgatccgt tcgctttgcc
     1561 aaattgcaac cgttcagtag tgactcgtgc gcagctcacc cgcatcgcat gcacctgcgt
     1621 ccctgacgcc tgagccgata cccacccccc gcagcttttc gtagactcag cgtgctcgcg
     1681 ccaattcgcg ccggttgcgg cccatcaggc gggcgccgat cggaatgcgg cagatgacgg
     1741 cggtgatcgc ccagctcagc gtcagcgtgc cggtgaacac gatcgcggcc ttcgcgacgg
     1801 cgaacaacgg cgcgtggagc aacatgtatt gcagccagat cacgaagacg tagtgaacga
     1861 agtagatgcc gtaggcgttg tccgccaggc tctcgaaaac cggccgttgt cgcccggcaa
     1921 agcgcaagaa caccgccgcg agcgcgagac acgcgctcgc cgggcacagc acgaggccga
     1981 ggctggcgac gaggcgcagg cccggcacca gcgttccgtg cacgatcagc gccgtgggca
     2041 cgacccacag caggaatgcg gcggcggtgc cgacgagcca acgcgcccat cgccgcgcca
     2101 gcactccgtc tccctcgagc aggcctcgat cgaacccgta tgctccgacg ccgagaccgg
     2161 cgaagaaata gacggcgtat tgcggcgcaa ggctcggttg aaacgcgaac ggaccgaatt
     2221 gcatccattg ccaaggctcg aacacggcgg cgagcggcag ataggccaac gccaagacgc
     2281 tcaccagcgc gatgaaaaac cggccgggat ggctgccggc cttggccgac agttcgccca
     2341 aggcttcgcc cgcgcgcggg gcgagccaaa agagcccggc ggccgcaagg ttgaacacga
     2401 gcagcagcca caagaaccac atcggcccgc tcggccagaa cggcagcgca atccagtgcg
     2461 accagaacgc cgaccagctc ggatcaaccg cggtgacccg atagactgga tagtaggcca
     2521 ccggcatcag cacatataac ccgattgcga acggcacgcc gaggcgcagg aagcggtcgt
     2581 agaggaactt cccgccgccc ttgcgcgaca ggctcggcca gacgaacagg ccggacaaaa
     2641 agaacagcag ctgcatcagg tagatgtact ggaacgcaca gaacaggtcg aagccgagcc
     2701 agcgcgcgct atcgacgatc gggttcgccc tccagccata aggggcaata tcgaacggga
     2761 gcgcagcatc gggttgcgag ttgaggtagg cgatgcatga atggaacgcc accaccatga
     2821 cgatggcaaa gccgcgcagg ttgctcaggg ccaggctcga cttggtcatg ctgcggactg
     2881 ctatcgcatt tcctcatgcc ggcaaagtag accgcgatat cgcg
//
LOCUS       DATLJT010001183         1746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1466135,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001183 DATLJT010000000
VERSION     DATLJT010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466135"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VM910_27230"
     CDS             <1..786
                     /locus_tag="VM910_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66248.1"
                     /translation="VDLFKALGDDPKAFTAQAVRAFVLERAKPHSRGRANTIATSTRA
                     FLRYLVATGQCPAGRDHAIPRFANRQLTSVPKFLVVEDIERAIAACDGENRLRDRAIV
                     LLLARLGLRASEVANLKLTDIDWKNGRVAIAGKSRRKEWLPLTQEVGDAIIAYVERAR
                     PRVTTSRLFFTDFAPIGPLTRVTVKCIVRRTLLRAGIKSVHRGAHVLRHSAATAMLRH
                     GVSLSGVATVLRHRSPAMTPHYAKVDLGLLSEIAQPWVGRLPC"
     gene            780..1643
                     /locus_tag="VM910_27235"
     CDS             780..1643
                     /locus_tag="VM910_27235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66249.1"
                     /translation="MLIDDVERYIALRRSLGFQLKKAARHLQTFAGLAAEKGERHIRA
                     VTVIEWAATAPTPDARNRRISDIARCARFLRAEDEGHEIPPTSLFATPKQRPAPYIYA
                     PDELVRMLDAAEKLRLLKSDPLRRRTFVMLFGLIATTGLRVSEAINLRLGDVLPGAVL
                     QIRETKFYKSRLVPLHATVAEALDRYLDIRRHFAGSDDHLFLSMEGKGLHYSTVRSAF
                     HRTLQLANIASGRARRPASTIFVTALRRESSSNAAQSATRLRDTLLRCRPISATPISR
                     IPTGILRRRRN"
BASE COUNT          348 a          562 c          493 g          343 t
ORIGIN      
        1 gtcgatctct ttaaagctct tggcgacgat ccgaaagcct ttaccgccca agccgtgcgg
       61 gcctttgttc ttgagcgcgc caaaccgcac agccgcggac gggccaacac aattgcgact
      121 tcaacgcgcg cgttcctccg atacttggtc gcgactggac aatgccccgc cggacgcgat
      181 cacgccattc cgcgcttcgc caaccggcag ttgacttcgg ttccgaagtt cctcgttgtc
      241 gaggacatag agcgagccat tgccgcctgt gacggtgaaa acaggctgcg cgaccgggcg
      301 atcgtcttgc tgctcgcccg tcttggcttg agggcgagtg aggtcgctaa cctaaaactc
      361 accgacatcg actggaagaa tgggcgggtt gccattgccg gcaagtcaag gcgaaaggaa
      421 tggcttcccc tcacacagga ggtcggcgac gcgatcatcg cctatgtcga acgagcaagg
      481 ccacgggtga cgacatcccg attgttcttc acggacttcg caccgatagg accgctcacg
      541 cgcgtcaccg taaaatgcat cgtgcgccgc acattgttga gagccgggat caagagcgtc
      601 catcgtggcg ctcatgtgct tcgccattca gcggcgaccg caatgctgcg gcatggcgtc
      661 agtttgagcg gtgtcgcaac cgtcttgcgt caccggtcgc cggcaatgac cccgcactac
      721 gccaaggttg acctcggtct gctgtcggag attgcccagc catgggtggg gaggctgcca
      781 tgctgatcga cgacgtcgaa cgttacatcg cgctccgccg ctctcttggc ttccaactca
      841 agaaggcagc gcggcacctg caaaccttcg ctggccttgc cgcagaaaag ggcgaacgtc
      901 atatccgcgc ggtaaccgtc atcgaatggg cggcgaccgc gccaacgccg gacgcccgaa
      961 atcgccgaat tagcgacata gcgcgttgtg cccgcttcct gcgcgccgag gacgaaggac
     1021 acgaaatccc accaaccagt ctcttcgcga cgccgaaaca gcgacctgca ccttatatct
     1081 atgcgccaga cgagttggtt cgcatgcttg acgcagccga aaaactccgc ctgctaaaat
     1141 ccgatccgct gcggcggcga acctttgtga tgctgttcgg gctcatcgcc actaccggac
     1201 tgcgggtctc cgaggcgatc aatctcagac ttggtgatgt gctgcccggc gccgtcctac
     1261 aaatccgcga aacgaagttc tacaaaagcc gcctcgtacc gttgcacgcc accgtggcag
     1321 aggctctcga tcgctatctc gatatacgtc gtcactttgc cggatcggac gatcatctat
     1381 ttttatcgat ggagggaaag ggccttcatt actcaacggt gaggagcgcc ttccaccgta
     1441 ccctgcaact cgccaatatc gcgtcgggcc gcgcacggcg gcccgcatcc acgatcttcg
     1501 tcacagcttt gcgacgcgag tcctcgagca atgcggcaca aagcgcgaca aggttgcgcg
     1561 acactttgtt gcgttgtcga cctatctcgg ccacgccgat atcgcgcata cctactggta
     1621 tcttgaggcg acgccggaat tgatgaccga catcgctgcg gcagccgaag cactggtcac
     1681 ggaggcggct ccatgacctt gctcgcccct ctgatcaccg gctttttgcg cgactatatg
     1741 ccccgg
//
LOCUS       DATLJT010001184         5733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_86487,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001184 DATLJT010000000
VERSION     DATLJT010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86487"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VM910_27240"
     CDS             complement(<1..230)
                     /locus_tag="VM910_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66250.1"
                     /translation="MLTQSAMRNGSRGQPETQPRRAPVTPAAMQAGTIELMGACMPFA
                     ANAEIYGENEPADYLYKVVSGTVRTYKVLVDGR"
     gene            591..983
                     /locus_tag="VM910_27245"
     CDS             591..983
                     /locus_tag="VM910_27245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66251.1"
                     /translation="MKNQGALSNSGLVVIVISDDLAVRNSLKFWLEIEGLAVRSYVSG
                     ADLLNAGDLARCDCYVVDQKTSATSALNLIAQLRDRHLTAPAILITSYPSPSLRQQAE
                     KAEVPIVEKPLVGNALLDKIRDVVGARG"
     gene            complement(1080..1532)
                     /locus_tag="VM910_27250"
     CDS             complement(1080..1532)
                     /locus_tag="VM910_27250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI66252.1"
                     /translation="MQGECPRGQSCKQRKRWGQASRRSRSNMIGKRIASLLIIAVSCS
                     LGVIAPAMAQSHPSPPSASEPPFALSPSQRRGFRLVSLHCARCHAIDKVSESPLAIAP
                     ALRTLHLKYPVSDLQRPLAQGIHPAMPRFRLEADQIEDVMAYLKTLEQ"
     gene            1736..3574
                     /locus_tag="VM910_27255"
     CDS             1736..3574
                     /locus_tag="VM910_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI66253.1"
                     /translation="MNQLTSLPVSAASASLPVLAASHAAQPKPSGSPTLEPGDLPSFD
                     TLNQLARAMTARLTQGVSPHAQYAAWFDWLSHLARAPGRQLELSLTAFIFGVRLLRLT
                     AHYGSESAALPPFSSGESDRRFSDPAWEKMPYLFWQQVFLAQESWWRSATHEVRGMSS
                     KNAARVSFMAHQFLDVMSPSNLPWLNPVIIEQTISESGANLVRGMSNFIEDMLCALAM
                     APKTSAHGFRVGEDIAATPGEVIYRNDLIELIQYKPTTTEVIAEPVLIVPAWIMKYYV
                     LDLLPHRSLVRYLVDRGFTVFMISWRNPTAAHRDLAFDTYRTAGVIAALNVVNAVVPG
                     RKVHACGYCLGGTLLAIAAATMARDGDHRLVTATLLAAQTDFSEAGELMLFVDESQVA
                     FLEDMMWDQGVLDPRQMTADFRTLRANDLIWSRIMREYLLGERDPTTDLTAWNEDPTR
                     MPYRMHSQYLRGLFLENRLTAGRYAVDGRVIALKDIHIPIFAVGTETDHIAPWRSVYK
                     LHLFTDNDLTFVLTNGGHNAGVVSEPGHKGRCYHVATRRPGARYVDSDTWLAAAIPQT
                     GSWWPEWTGWLQKHSGHERSAPPAMGAPAHGATPLIPAPGLYVHQR"
     gene            3736..5658
                     /locus_tag="VM910_27260"
     CDS             3736..5658
                     /locus_tag="VM910_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66254.1"
                     /translation="MKSKIIERLGESDILLPALIAQGLAANDRVKVRLSILQAAARHA
                     RDPAQVRFDLRDECRATGIDLVAMETLVNRAARLGDERITAPGLGDLRAAIWDDIGIM
                     MKAVKSGDAAQGDVLDERLSALKAADAPEPSGSAEPAEIARLTGLSDGDSVHRLVMDM
                     HKALNRLAAAHAEEVVAGAHVYGLLPEDRPAVEAFMRGLESTRKLKFDHPGLATTASR
                     SGDRLIIQNDIGETDAHVVVIAVEQDAVTVTYTDVHLARAKFFTGLFRNFSAQWSGLD
                     RKSAEGLGDEGVFYLVTGRYPTGGGNSRDVFLEAVGASVVFLIDWNKTRKILRAWVSP
                     SDAVHILDWAARHRFGHRAFLELGGSELVAAAVRHATPTRIGFGERLDRALGRDAAVD
                     FLKTVLRVSTEALLHGRSVRLVRDRIEADLVRHLERVDSTLLAIVIRQAGLAREVASE
                     IARYVARQRAGREVDASALAAHARRIEEKADRIAIEGRNEIARLEAGPIIEQLMNRVE
                     EAIDDLEQAAFIASIMPTGMSADVFKPLADLCSAAVSAAEAAATGVDAAAEVPEGKRL
                     DAEDALAAVGRLIDIEHLADAAEREVTTLVLRGGLSLPASLSVLELARAIERATDRLA
                     SFGHLLRQHVLADLST"
BASE COUNT         1050 a         1807 c         1773 g         1103 t
ORIGIN      
        1 cgcccgtcca ccaggacctt gtaggtgcgc accgtcccgc tgacgacctt gtagaggtaa
       61 tcggcgggct cgttctcccc gtagatctcg gcattggcgg caaacggcat gcacgcaccc
      121 atgagctcga tcgtgccggc ttgcatggcg gcaggtgtga ctggcgcgcg acgaggttgc
      181 gtttccggct gcccccggga accgttgcgc attgcgctct gcgtcagcat ggctcattcc
      241 ctccagttga tgagacattg ctaacgcgtg cgcgagggac cgagaattcc gataaaatcc
      301 ctccgggtta gcccggaggc agggcaggct gcaggctccc gatgaagaca ttcgtcacgg
      361 atttggccgc caaatcgagc ccgcagggcc agttcagcgc tgttatcgcg ggccggtgcc
      421 tgtcttaggt gaagggattg gccggcgcca tgattggcag gttggcaagg cgcccggctg
      481 tggccgaccc aggcgcttcc cggacttgag ccatatcaaa gcaacggtcc tcattttggt
      541 gctttttgca gcaccccacg accgcccgag ccgtgggcga aagcaaagcc atgaaaaatc
      601 agggcgcatt atcgaactcg ggacttgtcg tcatcgtgat tagcgatgat ctcgccgtgc
      661 gaaattccct caaattctgg ctcgaaatcg aaggcctggc tgttcgcagc tacgtgtccg
      721 gagcggacct attgaacgcg ggggacctgg ctcgctgcga ctgttacgtg gtcgaccaaa
      781 aaacgtccgc gacgagcgcc ttgaatctga tcgcgcagtt acgcgaccgg catctcaccg
      841 cgcctgcaat cctgatcacg agctacccta gcccatcgct gcgtcagcaa gccgaaaaag
      901 ccgaggtccc gatcgtagag aagcctctcg tcgggaatgc gctgctcgac aagattcgcg
      961 atgtagtcgg tgcacgcggt tagccatgcg gaggcaggtc ctctattgtc tggtcgcgat
     1021 ggggtcagac tgcccggagc ggctatgctc agcgagtctc agctcgctgc ggggctgcat
     1081 cattgctcga gcgtcttgag ataggccatg acgtcctcga tctggtcggc ctcaagtcgg
     1141 aatcgcggca tcgccggatg aatcccctgc gctaaaggcc gttgcagatc tgaaacagga
     1201 tacttgagat gcaatgtgcg tagcgcaggc gcgatcgcga gcgggctctc gctgaccttg
     1261 tcgatggcgt ggcatcgcgc gcaatggagg ctaacgagtc tgaaacctcg tcgttgcgag
     1321 ggcgatagcg cgaaaggggg ttcgctcgca gacggaggcg acggatgaga ttgggccatt
     1381 gccggcgcaa tgacgcccag gctgcagctg accgctatta tgagcaatga agcaatccgc
     1441 ttgccgatca tgtttgaccg ggagcgccgc gaagcttgcc cccacctttt cctttgtttg
     1501 cagctttggc cacgtgggca ttctccctgc atgctggggc ctttggctgc aaaacaaaat
     1561 catacgggtg tggctggact ttgacctcga tcaaatcttc cgcttgccct gtttgtaacc
     1621 tttttcaaaa acgcatcatt acgtgcggcg tcacatcagc gtcatgaatc ctgcatctat
     1681 tcaagtccga aactccgctg gcgtaaactt cgccggcgcg gaaaaatccg ttccagtgaa
     1741 ccaactgact tcgctccccg tttcggctgc atcggcttcg cttccggttt tggctgcatc
     1801 gcatgcggcg caaccgaaac catctggctc ccctactctg gaacccggtg accttccctc
     1861 gttcgacacc ctcaaccaat tagcgcgggc tatgaccgcc cgcctaacgc agggggtgtc
     1921 gccgcatgcc caatacgccg cttggttcga ctggttgtcg catcttgccc gcgcacccgg
     1981 gcgacagctc gagttgtcgt tgacggcgtt catcttcggc gtgcggctgt tacgcctcac
     2041 agcccactac gggtcggaga gtgccgcgct gcctccattt tcatcgggcg agtcggatcg
     2101 gcgcttttcc gatccggcct gggagaaaat gccttacctg ttttggcagc aagtcttcct
     2161 cgctcaggaa tcgtggtggc gcagcgccac ccacgaggtc cgcggcatga gctccaagaa
     2221 cgcggcacgc gtgagcttca tggcacatca gtttctcgac gtcatgtcgc cgtcgaacct
     2281 gccttggctc aatcccgtga tcatcgagca gaccatcagc gaatcggggg ccaatctcgt
     2341 tcgcggtatg agcaatttca ttgaggacat gctttgcgcc ctggcgatgg cgccgaaaac
     2401 ttcggcacat ggcttccgtg tcggcgagga cattgccgcc accccaggcg aggtcatcta
     2461 tcgcaacgac ctcatcgagc tcattcaata caagccgaca accaccgaag tgattgccga
     2521 gccggtgctg atcgtaccgg cttggatcat gaagtactac gtgctcgatc tcttgccgca
     2581 ccgctcgctc gtgcgctacc tcgtcgaccg cggctttacg gtgttcatga tatcttggcg
     2641 caaccccacg gctgcccatc gcgatctcgc attcgatacc taccgcaccg ccggcgtcat
     2701 cgccgcgctc aacgtggtca acgccgttgt tcccggacga aaggtgcatg cctgcggcta
     2761 ttgcctgggc ggcacgttgc tggccatcgc cgccgccacc atggcgcgcg acggcgacca
     2821 tcggctcgtc accgcgacgc tgctcgccgc ccagaccgac ttcagcgagg ccggcgaact
     2881 gatgttgttc gtggacgaaa gccaggtcgc attcctcgaa gacatgatgt gggaccaggg
     2941 cgtgctcgat ccgcgccaaa tgaccgcgga cttccggacg ctgcgcgcga atgatctgat
     3001 ctggtcgagg atcatgcgcg agtacctgct cggcgagcgc gacccgacca cggatctcac
     3061 cgcctggaac gaggacccga cgcgaatgcc ttatcgcatg cattcgcagt atttgcgggg
     3121 cctgtttctg gaaaaccgcc taaccgccgg ccgctacgcg gtggatggcc gagtgatcgc
     3181 gctcaaggac atccacatcc cgatcttcgc ggtcggcacc gagaccgatc acatcgcacc
     3241 ttggcgctcg gtctacaagc ttcatctctt caccgacaac gacctcacct tcgtgctcac
     3301 caacggcggc cacaacgccg gtgtcgtctc cgagcccgga cacaagggac gatgctatca
     3361 cgtcgcgacc cgccgtcccg gtgcgcgtta tgtcgattcc gacacctggc tggcggcagc
     3421 gataccgcag acgggctcat ggtggccgga atggaccgga tggctgcaaa agcacagcgg
     3481 acacgagcgc agcgcgccgc ctgccatggg cgcgcccgcg cacggcgcta cgccgctcat
     3541 tccggcaccc gggctatatg ttcaccaacg ctgacggctg atctcttgcg cgcttgatct
     3601 gccgcaacgc gcatcacaca tcgaaggcgc ccggctcttt gcgcaccgga tttgtgacag
     3661 ttttataaat gatctagctc aataacgagg ccgcccaaac gtgcgttagt aggcccggct
     3721 cgcaggtgcc catacatgaa atccaagatc atcgagcgac taggcgaatc cgacattttg
     3781 ctgccagccc tcatcgcgca gggtttggcg gccaacgatc gggtgaaggt gcgcttgagc
     3841 atcttgcagg cggcggcacg ccacgcgcgc gatcccgccc aagtgcgttt cgacctgagg
     3901 gacgagtgcc gcgccaccgg catcgatctc gtcgccatgg aaacgctcgt caatcgcgcg
     3961 gcccggttgg gagacgaacg gatcacggcg cccgggctgg gcgatttgcg agcggcaatt
     4021 tgggacgata tcggcatcat gatgaaggct gtcaaatccg gcgacgcagc gcagggtgat
     4081 gtcctcgatg agcggctgtc cgccctcaaa gcagccgatg cgcccgaacc ttcgggcagc
     4141 gccgagcccg ctgaaatcgc caggctgaca ggcctctccg atggtgacag cgtgcatcgg
     4201 ctggtgatgg acatgcacaa agctttgaat cgtctggccg cggctcacgc cgaggaggtg
     4261 gtggccggtg cgcatgtcta cggcctgttg cccgaggacc gccccgcggt cgaggcgttc
     4321 atgcgcgggc tcgaatcgac ccgcaagctg aaattcgatc atccgggcct ggcgacgacc
     4381 gcaagcaggt cgggcgaccg gctgatcatc caaaacgata tcggcgagac cgacgcccat
     4441 gtggtcgtga tcgcggttga gcaggatgcg gtgacggtca cctacacgga cgttcatttg
     4501 gcgcgcgcga agttcttcac cggcttgttt cggaattttt ccgctcaatg gagcggactt
     4561 gatcgcaaga gcgcggaggg cctcggcgac gagggagtgt tttatctggt caccggccgc
     4621 tacccgaccg gcggcggcaa cagccgggat gtttttctcg aagcggtcgg cgcgtccgtg
     4681 gtcttcctga tcgactggaa caagactcgc aagatcctgc gagcgtgggt atccccgagc
     4741 gatgcggtcc acatcctcga ttgggcggct cgccaccggt tcggccacag agccttcctc
     4801 gagctcggag gcagcgaact cgtagcggcg gccgttcgtc acgcgacgcc gacgcgaatc
     4861 ggattcggcg aacggctgga tcgcgcgctc gggcgcgacg cggccgtcga ctttctcaag
     4921 accgttctgc gcgtttcgac cgaagcattg ctgcacgggc gctcggtgcg cctcgtcagg
     4981 gaccggatcg aggcggatct cgtccgccac ctcgagcgcg tcgacagcac gctgcttgcg
     5041 atcgtgatcc ggcaggcagg gctggcgcgc gaggtcgcaa gcgaaattgc gcgctacgtg
     5101 gcgagacagc gggccggccg agaggtggac gcaagcgctt tggccgcgca cgctcgtcgt
     5161 atcgaggaga aggctgaccg gatcgcgatc gaaggccgca acgagattgc gcggctcgag
     5221 gccgggccga tcatcgagca gctgatgaac cgggtcgaag aggccatcga cgacttggaa
     5281 caggcggcgt tcatcgcttc gatcatgccc acaggcatga gcgcggacgt gttcaagccg
     5341 cttgccgacc tctgttccgc tgcggtctcc gccgccgagg cggctgccac cggcgtcgat
     5401 gccgcggccg aggttccgga gggaaagcga ctcgatgccg aggatgccct cgcagcagtc
     5461 ggccgtctca ttgacatcga gcatctggcc gacgccgccg aacgcgaggt gacgacgctg
     5521 gtgctgcgtg gcggcctctc cttgccggcg tcactctcgg ttctcgagct tgcgcgcgca
     5581 atcgaacgcg cgaccgaccg gctggcgagt ttcgggcatt tgcttcgcca gcacgttctt
     5641 gccgaccttt caacgtaaga gagaaagccg accaatgcag atcgtgcgca taggcgatgg
     5701 gccgactcaa caggtttcgg ccgagaccgt cgg
//
LOCUS       DATLJT010001185         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1696290,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001185 DATLJT010000000
VERSION     DATLJT010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696290"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(741..881)
                     /locus_tag="VM910_27265"
     CDS             complement(741..881)
                     /locus_tag="VM910_27265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66255.1"
                     /translation="MQAIDVKTLWVKKPRPRSDEMSKPWQPLKVLIAAEQAVSISGDV
                     WR"
     gene            complement(1583..2524)
                     /locus_tag="VM910_27270"
     CDS             complement(1583..2524)
                     /locus_tag="VM910_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018073855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI66256.1"
                     /translation="MITPEPDPMIARNLVGYGEFPPDPRWPGGAAIAVNFNLNVEGGG
                     ESTLANGDKVSEGMLNDIGVPTYAEVRIPLVESVFEYGSRRGVWRVLDAFRDFEIKTS
                     ILGVARALEQNLELVRAFVERGHEIVSHGYRWIDYATVPEEVEREHIRKAVDTLIALT
                     GTRPLGWMTGRPGPNTRRLLVEFGGFLYDRDSLADELPYWVSVSGQPHLVIPYSYEAN
                     DNRFNENSGFSTGNHFFTYMRDAFDLLRLEGQKGSPKLLSIGLHDRLIGRPGRCGGLI
                     KLLEHMRSCDDVWFCTGLEVAEHWHKNFAPRPDNWRA"
     gene            complement(2546..3673)
                     /locus_tag="VM910_27275"
     CDS             complement(2546..3673)
                     /locus_tag="VM910_27275"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI66257.1"
                     /translation="MERKRRVAVVGAGLGGLSIAGFLQRAGFPVTVYEQAPTFSRIGA
                     GIILSANITKALRRLDVEDALVAAGIKPHCYISRAWDTGATMYKIVFDAESERRYGGP
                     YLNIHRGDLHAVLERVVSKGTIWFDRRLTAIDETDGCMRLVFDNGETAEAEIVIGADG
                     IRSKVREHLLGDEPPRFVGAVAYRAIFSAARLRGFEIPDCTKWWGPDRHVLPYFMTSR
                     RDEIYVIGVVPWSRWDSEAASLPSSRDELIAAFAGFHQDLQRVLEAAQDVSVWPIYDR
                     EHDDRWTGGRIALLGDACHPMRPYMAAGGAMAVEDAAILSRCISEFHDLELAFRTYEA
                     TRVSRVADVHRISIENSWMRGPTEVDWFYQYDPCTAPLAQV"
     gene            complement(3803..3946)
                     /locus_tag="VM910_27280"
     CDS             complement(3803..3946)
                     /locus_tag="VM910_27280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66258.1"
                     /translation="MVFDAHWTRLYRLGEPSRQRLAMELKRLAEVVGEMGPEAPTPET
                     GGG"
     gene            4073..4192
                     /locus_tag="VM910_27285"
     CDS             4073..4192
                     /locus_tag="VM910_27285"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVI66259.1"
                     /translation="MRPPIHPASPACAGKTDERVVQAIHLTINGIAAGLRNSG"
     gene            4293..5270
                     /locus_tag="VM910_27290"
     CDS             4293..5270
                     /locus_tag="VM910_27290"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66260.1"
                     /translation="MKRRTFLYLSAATAVLRIASWAAWAQAYPARPVRVIVPFAPGGQ
                     VDVVGRLIAQRLSEQLGQQFYVENVSGAGSTIGAGRAAQAAPDGYTILIADGIAFTAN
                     PSLYAKVPYDATRDFDPVAAPATTMQVLAVNPSIAARSIQELVALIKANPGKYSYASA
                     GVGTGAHLTGELFRISLGLDLVHVPYNSGGQAIAAAVGGHTPISFGSPAATIPQVKDA
                     KLRALAVGGKKRVRELPEVPTMREVGFAAVECDTSMLVLVPAGVPREIIARLHDEVAR
                     AVALPDVEARLVALGFEPSTATADEVAALIKRELPKWAGVIRAAGIKPN"
     gene            5397..>5818
                     /locus_tag="VM910_27295"
     CDS             5397..>5818
                     /locus_tag="VM910_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI66261.1"
                     /translation="MTGLLEQHIAAVTGGASGIGRAIALGYAREGAHVVVLDANGNGA
                     AETAKAITGAGGKACSFVLDVTERDKCREVAAEIGETVGRVSVLVNNAGINRRNAFTA
                     DPDTVIKDWQDIMAVNLYGVFNVTHAFLGHLRATRGRIV"
BASE COUNT         1177 a         1763 c         1764 g         1114 t
ORIGIN      
        1 gttcatcacg ctgctcggtg gtgcggccgc gtggccgctc ggagcgacct atcgagaggt
       61 ccgagcggca ggctgatcca tcttggccat cattggcgag cttgaatccg caggcatggc
      121 atgtgcggat acgagcgcct ggtctagctg ccacaccttc atgtctttgc cgcaggcttg
      181 gcctaaccag agttccattg cttattttgt ctcgactcgc tctgggtaaa atcgcagaat
      241 tagagcgacc gagacgctac cccaaagcgt tcggcactac atccaacgtg ttgtctggga
      301 ccccttcgag cgtcctgtgt cctcgctgcg acgcatgtcg cgcgaaagaa tcgccttcaa
      361 tggttggctg ttcagcagcc gagcaatcag ggaggagcct atgccccggt ataagtgcaa
      421 aagcattgcg gcgctcgaca tagctctcgg cggcttgcca gctaagatgc aggttgaggc
      481 ggacgtagcc actggcgtgt ctgcaaagac cgttggagag cttcggaggg tgacaacttg
      541 gtccgagaat ttggtgctaa caatcccgca ggaacgtcat cccgaaagcg cagtaaagat
      601 cagcaaggct agcgtagcga cacgcacctc gccgaaaccg tagtacccgg cgcgagggac
      661 aaacggtact agccactctt gcaacgtgcg ccgcggccga accttccgca gatccgtcgc
      721 ggcattctcg cgctggccgg ctaacgccac acatcacccg agatgcttac cgcctgttcg
      781 gcggcaatca gaaccttgag cggctgccac ggcttggaca tctcgtcgct ccgcgggcgg
      841 ggctttttga cccacaacgt tttcacatca atcgcctgca aatttttgct ccttgtgtcg
      901 ggtgtctgac caagggcagt ctcacggcgt gtcaggatgg gttcagcacg gggaagctaa
      961 ggcaggcgcg agtatggctt ttctcctgag cgaccttctg ctgtattctg gtcactggga
     1021 tggtgatcgc gattgcgagc ctactcagct aactgtcgta ctcggatgga ggaaatagtt
     1081 ctctcgacgt aggaagtcga gcggcgcaaa aaaagagccc cgcgtgagcg gggctgagta
     1141 tttcggagga acgtagaacg accgcatatg cggtacgatc cacaaatagg aggttagctc
     1201 ggccattgcg ctcgctatgt gatggcgatc acagaagtgc gcttttttta cagctgagtt
     1261 gttgcgagcc tgctcggctg actgtcgtgg ctcggatatg gcaacagcct aaccgatgca
     1321 gcggccaaac taccgccgcg cgccatgcgg aagcggtgga tacgtcgacg agccacgtcg
     1381 gattccgatg agccgctacg gaaacatgtc ggcagggcgc ccctcaccgg gcgccctttt
     1441 tatttccgga tcgctcgacg cgccaccgga gcattggcgg cgctagttca gccacgtcgc
     1501 ggggtcgcgc ttggtatcga cagcaggtcg acgtgcgtta tagccggatc ggtttcatga
     1561 tcttgcggtc atcaaaggac attcaagcgc gccaattgtc cgggcgtggg gcaaagtttt
     1621 tgtgccaatg ctcggcaact tcaagaccgg tacaaaacca aacgtcgtca cagctgcgca
     1681 tatgttcgag caacttgatc aagccaccgc aacgtccggg gcggccgatc aatcggtcat
     1741 gaagaccaat cgacaaaagt ttcggcgacc ccttttggcc ttccagtcga aggagatcaa
     1801 aggcatcgcg catataggtg aaaaagtgat tgccggtcga gaagccgctg ttctcgttga
     1861 agcgattgtc atttgcctca tacgagtagg ggattaccaa gtgcggctgt ccactcaccg
     1921 aaacccagta cggtaactcg tcggccaaag agtctcggtc gtacagaaaa ccaccgaact
     1981 caaccaggag ccgccgcgta tttgggcctg gtcgtcctgt catccaacca aggggacgag
     2041 tgccagtgag cgcgatcagc gtatccacgg ctttcctaat atgttcgcgc tcgacttctt
     2101 ccggcaccgt ggcatagtcg atccagcgat agccatgact cacaatttca tggccgcgtt
     2161 ccacaaacgc tctgacaagt tccagatttt gctcgagcgc tctcgcgacg ccgaggatgc
     2221 ttgtcttgat ctcgaagtcc cgaaaggcat ccaatacgcg ccacacaccc cgtcggctgc
     2281 cgtattcgaa gacggattca acaaggggga tgcgaacctc ggcataggtc ggcacgccga
     2341 tgtcgttcag catgccctcg gagaccttat ccccattcgc caaggtggac tcgccgccgc
     2401 cctccacgtt cagattgaag ttgaccgcaa tcgcggcgcc gccgggccat cgcgggtccg
     2461 gcggaaattc accgtatccg accaggttgc gagcaatcat cgggtccggt tcaggggtaa
     2521 tcatgtggtc ctctgtggaa agagcctaca cttgcgccaa gggggcagtg catggatcgt
     2581 attgatagaa ccaatcaact tcggtcggcc cgcgcatcca gctgttttcg atggaaatgc
     2641 ggtgaacatc ggcgacccgt gaaacccgcg tcgcttcgta ggttcggaat gcgagctcaa
     2701 gatcgtgaaa ttccgatatg cagcggctga gaattgcggc atcctccacg gccatcgcgc
     2761 ctccggccgc catataaggc cgcatcgggt ggcaggcgtc tccgagaaga gcaatccggc
     2821 cgcccgtcca gcggtcgtcg tgctcccgat cgtaaatcgg ccaaacgctg acatcctgtg
     2881 cggcttcaag gacgcgctgc aggtcctgat ggaagccggc aaacgccgca atcaattcgt
     2941 cgcgcgagga tggcagcgaa gctgcctcac tgtcccagcg gctccaggga accacgccga
     3001 taacatagat ctcatcgcgt cgacttgtca tgaaataggg caggacatga cggtccggcc
     3061 cccaccactt cgtgcagtcc ggaatctcga agccgcgtaa tcgcgccgcg gaaaagatcg
     3121 cgcggtacgc cacggcgccg acgaagcgcg gcggctcgtc cccgaggaga tgttctctta
     3181 ccttcgaacg aatcccatct gcgccgatga caatctccgc ctcggcagtt tcgccgttgt
     3241 caaaaaccag gcgcatgcat ccatctgttt catcgatcgc cgtgaggcgg cggtcgaacc
     3301 agatggtccc tttgctaaca acgcgttcga gcacggcatg aaggtcgccg cgatggatgt
     3361 tcagatatgg tccgccgtag cgtctttcgc tttcggcatc gaagacgatc ttgtacatcg
     3421 ttgcgccggt atcccatgcg cggctgatat aacagtgcgg cttgattcca gcggcgacga
     3481 gggcatcctc aacatcgagc cttctcagcg ccttcgttat gttggcgctc aaaatgattc
     3541 cggcgccgat gcgggaaaat gtcggcgcct gctcgtagac tgtgaccgga aagccagcac
     3601 gctgcaggaa tccggcgatg gataagccgc cgagtcccgc gccgaccaca gcgacgcgac
     3661 gtttacgctc catgctattg ctctgaagaa ccaagacgag ggcgctgctg gccatttttg
     3721 gcggatgcaa agagaaccaa ggcccgcccc gaaatcaatt cgacgtcgaa caaattgacg
     3781 ctacgggccg cgttcggcgc tgtcaaccac cgccagtctc gggcgtcggt gcctccgggc
     3841 ccatttcacc gacgacctcg gccaagcgtt tcagttccat cgcaagccgt tggcgtgagg
     3901 gctcgccgag gcgatacagc cgcgtccagt gcgcatcgaa aaccacgggg atcaccttgt
     3961 tgacgagcga atatgtcgga gcgtcgatcg atcactggcc gacttttgca acatagggta
     4021 attccgggat cgtgccatac gaaggcacac ggaatcagtt ctccaggaac atatgaggcc
     4081 acccattcat ccggcctcgc ccgcatgcgc cggcaagacc gacgagcgcg tggtgcaggc
     4141 aatccatctg accatcaatg ggatagccgc agggctgcgc aacagtggct agtcccaagc
     4201 agagcagtga accgcgccac cgcatcaaac cgatatccgg ccgtcgagaa gtgcgatagc
     4261 ctgctcgctc catgtcgcat ggggagcgcg agatgaaacg cagaaccttt ctatatctga
     4321 gtgccgccac cgcggtcctg cgcatcgctt cgtgggcagc atgggcgcaa gcctatccgg
     4381 cgcggccggt gcgggtgatc gttccgttcg cccccggtgg gcaggtggat gttgtcgggc
     4441 gcctgatcgc gcagcggttg tccgagcagc ttggacaaca gttctacgtc gagaacgtct
     4501 ccggcgccgg tagtacgatc ggcgcgggcc gggcggctca ggcggcacct gacgggtaca
     4561 cgatcctcat tgcggatgga atcgctttca cggcaaatcc cagcctctac gccaaggtcc
     4621 cgtacgacgc cacgcgcgat ttcgacccgg tggcggcccc ggccaccacg atgcaggtcc
     4681 tcgcggtgaa cccttcgatt gcggcgcgct ccattcaaga gctggtcgcg ctgatcaaag
     4741 caaacccggg caaatacagc tacgcttcgg ccggcgtcgg aacgggcgcg cacctcacgg
     4801 gcgagctgtt ccgcatctcg ctcggcctcg atctcgtgca cgtgccttac aatagcggag
     4861 ggcaagcaat cgcagccgcc gtcggaggtc atacgcccat ttctttcgga tcgccggccg
     4921 cgactattcc gcaagtgaag gatgccaagc tgcgcgctct cgccgtcggc ggcaagaaac
     4981 gcgtgcgcga gctcccggag gtcccgacca tgcgcgaggt gggctttgcc gccgtggaat
     5041 gtgatacgtc gatgctggtt ctggtgccgg ccggcgtgcc aagggagatc atcgcgcggc
     5101 ttcacgacga ggtcgcccgc gccgtcgcat tgccggacgt ggaggcgcgg ctcgttgcgc
     5161 tcggctttga gccgtccacg gcaactgcgg acgaggttgc ggcgctgatc aagcgcgagc
     5221 ttccgaaatg ggccggcgtc atccgcgcgg cgggcatcaa gccgaattga ggttgcgctt
     5281 ggtcgcgcaa tgaaacgccg tcgccctgct tgagtgacga cattacaatg acacgcttga
     5341 ctgcatgacg cgatgacggc ggagtccgct ggcacgatat tggaggggga gggaaaatga
     5401 cgggccttct cgaacaacac attgcggcgg tcaccggcgg cgcctccggc atcggccggg
     5461 cgatcgcgtt gggctatgcg agggagggag cacacgtggt cgtgctcgac gccaacggca
     5521 acggcgcggc cgagaccgcc aaggccatca caggcgccgg cggcaaggcc tgcagcttcg
     5581 tgctcgacgt gaccgagcgc gacaaatgcc gcgaggtcgc cgcggagatc ggcgagacgg
     5641 tcggccgggt ctccgtcctg gtcaacaatg ccggcatcaa ccgccgcaac gccttcaccg
     5701 cggatcccga cacggtgatc aaggattggc aggacatcat ggcggtgaac ctttacggcg
     5761 tgtttaacgt aacgcatgcc ttcctgggac acctccgcgc gaccaggggc cggattgt
//
LOCUS       DATLJT010001186         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1696297,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001186 DATLJT010000000
VERSION     DATLJT010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696297"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..797)
                     /locus_tag="VM910_27300"
                     /pseudo
     CDS             complement(49..797)
                     /locus_tag="VM910_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1262..1924)
                     /locus_tag="VM910_27305"
     CDS             complement(1262..1924)
                     /locus_tag="VM910_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI66262.1"
                     /translation="MIERPARPLASAEEPIVFVVDDDQSLREALRSLLRSVSLRVETF
                     GSAAEFLRSELPDVASCLVLDVRLPGVSGLDFQAELAKANIHIPVIFMTGHGDIPMSV
                     QAMKAGAVDFLAKPFRDQDMLDAVAAAIERDRSRRKDEQRLSDVRGRFESLTEREREV
                     MGLVTAGLMNKQVAGELGLSEITVKIHRGHVMRKMAARSLADLVRMAEVLGVRPSKPD
                     SR"
     gene            2244..2387
                     /locus_tag="VM910_27310"
     CDS             2244..2387
                     /locus_tag="VM910_27310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66263.1"
                     /translation="MKALIAALALVTLIASPTFAQTAPTANSPTCGGYGWGPSSPCYG
                     SNP"
     gene            2495..2746
                     /locus_tag="VM910_27315"
     CDS             2495..2746
                     /locus_tag="VM910_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66264.1"
                     /translation="MLGKVRITSIAAAALLASPAFAAPPFAQPVQVEQIDDQRVITEV
                     VVNGHLIEGADEAPANELRPVPNWIDGSGALDPARRHSY"
BASE COUNT          499 a          904 c          818 g          564 t
ORIGIN      
        1 actgggtccg gtgctaatcc gcccccagtt gtgtccgaac agatcgactc agacggcgag
       61 tgcttccagg atcaggttcg tgatcgcatc gggatgtgag atcagcgcga ggtggctcgc
      121 ctcgatctcg atcgtcttcg cacccattcg cttggccatg aagcgctcca ggtcgggatt
      181 gatcgtccgg tcttgggttg acacttggtg cgccaggcgg cattcgtcgt cttttccgtc
      241 gtcagcgatt tcttgaacgg ctgctgcacc gcgtagagga cgcgcgcctc ctccggcggc
      301 aggtcgcccg cgaaatcgtg cagaaaagcc tcctccgaca actggcccca atcgtcgttg
      361 aagacaatgc cggctgtggc cgggggcgtc ggatagttct tcgccagcgc cgtatagtcc
      421 tcgcccgcat cgggtgcccg cgcagcgacg taaacgaggg cggtgacttt cgggtccacc
      481 cccacatccg tcacgatcat tccggagaag gaatggccga cgaggaccgt cgggcccggt
      541 tgtgccgcga ttgcccgtcg cgcggcctgc gatgcctcat gcagcgaggt cagagggttc
      601 tgcacgctgg tgcagttgac gcccttctgc tggaggcggg cgatgacctt cgaccagcag
      661 gaaccgtctg cgaagagtcc gtgtacgaag acgacatttt tcgtcttcgg cgatgtctgg
      721 gcgctggcga ttcgattgag caagggaagg gcggcgctcg cagcggcagc cccgatcagt
      781 gtccggcggt tcatcatagc ctcctattga acgacggccg atttggctcc gtcatgggaa
      841 aattgccccg cgtgtttcca gcaatatcgg gctcatccag atctgtgttt gccgaaattg
      901 cttcggcccc gtcgcatgct gcactgtatg gcgggcgatc cggcaaccgc tggcccgatc
      961 ttgtcgtcgc tgtttcggaa ttgcggcatt ccgccatgct gcgcccacgc acccgatacg
     1021 ccgccaattc catgacgcat gccaaacctg aggatcatcg tgatgggcgc cacgtccggt
     1081 cagggttatg ttcgaccgag tcagccggtt ttgccgcctg gcccacttcc gcttcgcgcc
     1141 gaaacaaact cgccgccaac gacctagcct tcatccagct ggcttcaaat cagggtttgg
     1201 ctgcgcgcta atgagtccac gcccttagat gaaaattctt ggcagctcgg tcgctttgac
     1261 cctatctgct gtccggcttg ctcggccgaa cgccaagcac ttccgccatt ctcaccaggt
     1321 cggcgagcga gcgcgcggcc attttccgca tgacgtgtcc gcggtggatc ttgacggtaa
     1381 tctcgctcag ccccagttcg ccggcgacct gcttgttcat caggccagca gtgacgagac
     1441 ccatcacctc gcgttcgcgc tcggtcaagc tctcgaagcg tccccgcacg tccgatagac
     1501 gctgctcgtc ctttcgccgg ctgcgatcgc gctcaatcgc cgcggcgacc gcgtccagca
     1561 tgtcctgatc gcggaagggc ttggcgagaa aatcgaccgc gccggctttc atggcctgga
     1621 cggacatggg gatatcgccg tggccggtca tgaatatgac gggaatatgg atgttcgcct
     1681 tggcgagctc ggcttggaaa tcgagcccgc tcacccccgg caatctgacg tcgagcacga
     1741 ggcagcttgc cacatcggga agctcgcttc gcaaaaactc cgcggccgac ccgaacgtct
     1801 cgaccctgag actgaccgaa cgaagcaggc ttctgagcgc ctcgcgcagc gattgatcgt
     1861 cgtcgacgac gaacacgatc ggctcttcgg cgctagcgag cgggcgagct ggacgctcga
     1921 tcacgacatt ctctccgggc agccgggtct aaagccaaag atagcacgtc cacgcacgat
     1981 atcgaaccgc tcggcgctgc gatcgctgtt ccctctttcg ttcgccgccg ccgctccccc
     2041 cacgcatggc gaatgggcct ttcacggcag cgcccaaacc aaagtataag gccgtcgatc
     2101 cgttcggtta tcggatcaat cccaaaatat agttcatccg ccgatcgtga ccggcctatc
     2161 tccatggtga ctgcaaagca ccggccgtgg gccgaccgct cttgcaggaa ctaggacaac
     2221 cgtccgtacc atggagaagc atcatgaaag cgcttatcgc tgctctcgcg ttggtcacgc
     2281 tgattgccag cccgaccttc gctcagactg cgcccaccgc caacagtccg acctgtggcg
     2341 gctacggctg gggaccaagc tcgccctgct atggttccaa tccatagcgc cgcttgggcg
     2401 aggactccgg ttcaagccca cgcgatggct cgactatgcg gccggccctt gcgccggccg
     2461 cttaaggaaa gttcgcgctc tgttggagat gaaggtgctt gggaaagtac gcatcacatc
     2521 gatcgccgcg gctgcgctgc tggcctcgcc cgcattcgcc gctccgccat tcgctcaacc
     2581 tgtgcaggtc gagcagatcg acgatcagcg cgtcataaca gaggtcgtgg tcaacggcca
     2641 cctcatcgag ggcgctgatg aagcgcccgc caacgagctc cggccggttc caaattggat
     2701 cgacggcagc ggcgctctcg atccagcgcg ccgacactca tactaaggtg atgaaatgtt
     2761 tttctcgctc ttttggacag cttac
//
LOCUS       DATLJT010001187         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_317780,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001187 DATLJT010000000
VERSION     DATLJT010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317780"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VM910_27320"
     CDS             complement(<1..309)
                     /locus_tag="VM910_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI66265.1"
                     /translation="MKLTRLRLQGFKSFVEPTDFLIEPGLTGVVGPNGCGKSNLVEAL
                     RWAMGETSHKSLRAADMDAVIFSGSGNRPARNHAEVVMTIDNSDRTAPAAVNDSQVLE
                     I"
     gene            complement(577..1233)
                     /locus_tag="VM910_27325"
     CDS             complement(577..1233)
                     /locus_tag="VM910_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI66266.1"
                     /translation="MIITRRAFTAALSFTGLAALAGFSPLRLITEAVAQNATDVAKPV
                     SLPDMALGRADAAVTITEYASMTCPHCAAFNADVFPKIKSEYIDTGKIRYVFREFPLD
                     IKAAAGSVLSRCIAKDDANKYFAVTDLLFKTQADWVMKNTTESLIRIGKQAGLTQDQI
                     ETCLRDQSLLDKLAADQKYAAEVLKVDSTPTFFINGEKIKGETSIEEFEKKINPLLKS
                     "
     gene            complement(1348..1827)
                     /locus_tag="VM910_27330"
     CDS             complement(1348..1827)
                     /locus_tag="VM910_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HVI66267.1"
                     /translation="MNKPGRSSAKPLSLLLSDVFSEAYAKQGFAARELVTRWAEIAGA
                     DVAAHSEPLKLQWPRPVEGRPQEPATLVLRVEGPMALEIQHKSDVILERVNRFLGWSA
                     VGRLALRQAPLSRRDRPKPPSPPDPEAVARIAETLDAVEDEQLRAALARLGAAIKRN"
     gene            1855..>2689
                     /locus_tag="VM910_27335"
     CDS             1855..>2689
                     /locus_tag="VM910_27335"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVI66268.1"
                     /translation="MSLAGAAIRQKQSSSSGRDDATNNRPVLLLAWYDRHRRKLPWRA
                     EAGARADPYRVWLSEIMLQQTTVKAVGPYFEKFVTRWPDISALARASLDDVLRMWAGL
                     GYYSRARNLHACAVAVTRDHGGVFPDTEEGLRGLPGIGPYTAAAIAAIAFDRRTMPVD
                     GNIERVVSRLFALEEPLPQAKSRIRQLAATLIGPARVGDVTSRAGDSAQALMDLGATI
                     CTPKKPACVLCPLSGDCAARALSTQETFPRKAPKKSGALRRGAAFVVTRGDELLVRRR
                     PE"
BASE COUNT          450 a          850 c          892 g          497 t
ORIGIN      
        1 gatttcgagg acctggctgt cgttgacggc agccggcgcg gtgcgatcgg agttgtcgat
       61 cgtcatcacc acttcggcat ggttgcgcgc ggggcggttg ccggaacccg agaagatcac
      121 cgcgtccatg tcggcggcgc gcagcgattt gtgcgaggtc tcgcccatcg cccagcgcag
      181 cgcctcgacc agattggatt tgccgcagcc gttcggaccg acgacgccgg tcagtcccgg
      241 ctcgatcaaa aagtcagtcg gttcgacgaa cgacttgaaa ccttgaaggc gaagacgcgt
      301 gagcttcata aagcagtttc tctgttggca gcgcgaatct ccctgccggg acaataccat
      361 agaaggcaat ctcggtatgg ggggagaggc gtcatgagcc gcttatgggg cgcgacaatg
      421 gctgcgtcgc gcgttgaggg caacgggccg aacggtcttt tcccaagcct tttgctgggg
      481 ttatcagcac agatgaatct tgaccgcaag cggccggaga atcagcacct gtccggcgac
      541 tcgatgtttc ccgcgaacgc ggcccggcgc ggtcggtcag ctcttcagca gcgggttgat
      601 cttcttctcg aattcctcga tcgaggtctc gcctttaatc ttctcgccgt tgatgaagaa
      661 agtcggggtc gaatccacct tcaacacctc ggctgcatat ttctggtcgg cggcaagctt
      721 gtcgagcagc gactgatccc tcaggcaggt ctcgatctga tcctgagtga gccccgcctg
      781 cttgccgatc cggatcagcg attccgtggt gttcttcatc acccagtcgg cctgcgtctt
      841 gaacaggaga tccgtcaccg caaaatattt gttggcgtcg tccttggcta tgcagcgcga
      901 cagcaccgag ccggcggccg ccttgatgtc gagcgggaac tcgcggaaga cgtaacggat
      961 cttgccggtg tcgatatatt ccgacttgat cttcgggaac acgtcggcgt tgaaggctgc
     1021 acaatgcggg caggtcatcg aggcgtattc ggtgatggtc acggcggcat ccgcccggcc
     1081 gagcgccatg tccggtagcg acaccggctt tgccacgtcg gtggcgtttt gcgccacggc
     1141 ttccgtgatc agccgcagcg gcgagaagcc ggcgagcgcg gcaaggccgg tgaacgacag
     1201 ggcggcggtg aaggcgcggc gcgtgatgat caaggctctc tcccggattg gcgcgacttg
     1261 cgcccgtatc ttcaaaatac ggtgcgttcg ctagctcgaa atcccagggg aggcaatggt
     1321 cgcgcgccgc cggttgcggc cagggcgtca atttcgcttg attgcggccc cgagacgggc
     1381 cagtgccgcg cgcaattgtt catcttcgac cgcgtccagg gtttcggcga tcctcgccac
     1441 ggcctcagga tccggcgggg agggtggttt ggggcggtcc cgacgcgaca gcggcgcctg
     1501 ccgcagtgcc agccggccga ccgcgctcca gccgagaaaa cggttgacgc gttcgaggat
     1561 gacgtccgac ttgtgctgaa tctccagcgc catcggaccc tcgaccctca gcaccagcgt
     1621 cgccggctcc tgcggccggc cctctaccgg gcgcggccat tgcaatttga gcggctcgga
     1681 atgggccgcg acatccgcgc cggcgatctc cgcccagcgt gtcaccagct cgcgcgcggc
     1741 gaatccctgc ttggcatagg cctcggagaa gacgtcgctg agtaacaggg acaacggctt
     1801 tgcgctgcta cggccgggct tgttcatgga cggacgctct gcctacatag gcccatgagt
     1861 ctagcaggcg cagccatccg gcaaaagcaa agctcttcgt ccggacgcga cgatgcgacc
     1921 aacaaccggc ccgtgctttt gctcgcctgg tacgaccggc atcggcggaa gctgccgtgg
     1981 cgggccgagg ccggcgcgcg cgccgatcct tatcgggtct ggctgtcgga gatcatgttg
     2041 cagcagacca ccgtgaaggc ggtcgggccc tatttcgaaa agttcgtcac gcgttggccc
     2101 gacatctcgg cgctggcgcg cgcgtcgctt gacgatgtct tacggatgtg ggccgggctc
     2161 ggctattact cgcgcgcgcg caatctccat gcttgcgcgg tcgccgtgac gcgcgaccat
     2221 ggaggtgtgt ttcccgacac cgaagagggc ctgcgtggac tgccggggat cggaccctac
     2281 acggcggccg cgatcgccgc gatcgccttc gaccgccgca ccatgccggt cgacggcaat
     2341 atcgagcggg tggtgtcgcg gctgttcgca ctggaagagc cgctgccgca ggccaagtcg
     2401 cgcatccggc aattggccgc gacgctgatt ggaccggccc gcgtcggcga cgtgacgtca
     2461 cgcgccggcg acagcgcgca ggcgctgatg gatctcggcg caacgatctg cacgccgaag
     2521 aagccggctt gcgtgctgtg cccgctctcc ggcgactgcg cggcacgcgc gctctccacg
     2581 caggagacct ttccgcgcaa ggcgccgaaa aaatcgggtg cgttgcgccg cggcgcagcc
     2641 ttcgtggtca cgcgaggcga tgaactcctg gtgcgcaggc gccccgaaa
//
LOCUS       DATLJT010001188         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1696429,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001188 DATLJT010000000
VERSION     DATLJT010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696429"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VM910_27340"
     CDS             complement(<1..1029)
                     /locus_tag="VM910_27340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI66269.1"
                     /translation="MSESENIVVETAEKIFSDLADAQEVNRDKQGAWKAPLWRALTEA
                     GLPLSWVSEELGGSGASVAEGFGVLGAAGRFAVAVPLAETMLAGWLLAQAKIKSPEGE
                     MTVAPASPKDRISLNADGTLSGRARGVPFAKAAKHIAVLASGNGGASIALVDAAAVRI
                     ETGLNLASDNSDSVIFDRVAPLAIKPAPKAIDQTALMLMGGVARSLQIAGALESMLDI
                     SVRYSNERIAFEKKISKFQAVQHNLAKLAGESAAALAAATSAADTLANAKSLDDDAVF
                     LEAVAAKIRCSEAAEKGSAIAHQVHGAIGFTIEHILHRYSLRALAWRDDFGSESYWAV
                     ELGKRVAAR"
     gene            1284..2066
                     /locus_tag="VM910_27345"
     CDS             1284..2066
                     /locus_tag="VM910_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI66270.1"
                     /translation="MSKDGLCAIVTGSASGLGAATAGLLAEDGARIVVNYSHSKAEAE
                     ATADLCRKRGAEVVVVQGDVSRDEDCRKIVAAATPWGRLDVLVNNAGTTKHVPHDKLD
                     ELSAEDFQRIYAVNTIGPYQMIRAARSLLEASSKATGRPGAVVNVSSVAGIAGNGSSV
                     AYAASKGALNTMTLSLARALAPLIRVNTVCPGYIDTPWFTKGRGEAGAKQVRDMVVAR
                     VPLKVASTAEDIARVVCFLASPASSNMTGELVRMDAGMHLIV"
     gene            complement(2130..2405)
                     /locus_tag="VM910_27350"
     CDS             complement(2130..2405)
                     /locus_tag="VM910_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVI66271.1"
                     /translation="MSGIIWVIIVGFVAGIIARMLSPGPNNPSGFILTTVLGIAGAFL
                     ATWIGQAIGHYGPNQGAGFITATIGALLVLFIWNRLVASGVIRDFNG"
BASE COUNT          437 a          809 c          803 g          426 t
ORIGIN      
        1 gcgcgcagcg acgcgcttgc cgagctcgac cgcccaataa ctctccgagc cgaaatcgtc
       61 gcgccaggcg agcgcgcgca gcgaatagcg gtgcaggata tgctcgatgg taaagccgat
      121 cgcgccgtgg acctgatgcg cgatggcgct gcccttctcg gcggcttccg agcagcggat
      181 ctttgccgcg accgcctcca ggaacaccgc gtcgtcgtcc aatgattttg cattggcaag
      241 cgtgtcggcc gccgaggtcg cggcggcaag cgccgcggcg gattcgccgg caagcttggc
      301 gaggttgtgc tgcaccgcct gaaatttcga gatcttcttc tcgaaggcta tgcgttcgtt
      361 ggaatagcgc acgctgatat cgagcatcga ttccagcgcg cccgcgatct gcaggctgcg
      421 cgcgacgccg cccatcagca tcagcgccgt ctggtcaatt gccttcggcg ccggcttgat
      481 cgcaaggggc gcgacgcgat cgaagatcac gctgtcgctg ttgtcgcttg caagattgag
      541 ccccgtctcg atcctgactg cggccgcatc gacgagcgcg atcgacgcgc cgccgttgcc
      601 gctcgcaaga accgcgatgt gctttgcggc tttcgcaaag ggaacgccgc gggcgcggcc
      661 ggagagcgtg ccgtcggcat tgaggctgat acggtccttc gggcttgccg gagcgaccgt
      721 catctcgccc tcgggagatt tgatcttcgc ctgcgcgagc agccatccgg ccagcatggt
      781 ttcggcaagc gggacagcga ccgcaaagcg tcccgccgcg cccagcacgc cgaatccttc
      841 ggcaacagac gcgcccgagc cgcccaattc ctcggacacc caggacagcg gcaggccggc
      901 ctcggtcagc gcccgccaca gcggcgcttt ccacgcgcct tgcttgtcgc ggttgacctc
      961 ctgcgcatcg gcgagatcgg aaaaaatctt ctccgcggtc tcgacgacga tgttctcgct
     1021 ctccgacacg gcgttccccg gtgtgatcgt cttgtagcag ccctgttctt ggcttgcatg
     1081 atggggaaaa gcatctcccc tgacaagcgg ccatgacggc atgcgcgcct gcggggctga
     1141 catgaggcgt gtggaacgga ttgacgttcg cggccccgca gaccgcgata ccgtgaggcc
     1201 aattccgcgc aacggagttt cgcgtttcgc ggcctggtat gctaatcgca agcggacgga
     1261 taagagacaa cccggggaaa cgcatgtcaa aggatggttt gtgcgcgatc gtgaccggct
     1321 cggcatcggg ccttggcgcc gcgaccgccg gtcttctggc cgaagacggg gcacggatcg
     1381 tggtcaacta ttcccacagc aaggccgagg cggaagcgac cgccgatctc tgccgcaagc
     1441 ggggcgccga agtcgtcgtc gtgcagggcg atgtttcgcg cgacgaggac tgccgcaaaa
     1501 tcgtggcggc ggcaacgcca tggggccggc tcgacgtgct cgtcaacaat gccggcacca
     1561 cgaaacatgt tccgcatgac aagctggacg agctttcggc ggaagacttc cagcgcatct
     1621 atgcggtgaa caccatcggg ccgtatcaga tgatccgcgc cgcccgatcg ctgctggagg
     1681 cttcctccaa agccacgggc aggccgggcg ccgtcgtcaa cgtgtcctcg gtcgcaggca
     1741 tcgcgggcaa cggctcctcg gtcgcctatg ccgcgagcaa gggcgcgctc aacaccatga
     1801 cgctatcgct ggcgcgggcg ctcgcgccgc tgatccgcgt caacacggtt tgccccggct
     1861 atatcgacac gccctggttc accaagggtc gcggcgaagc cggcgccaag caggtgcgcg
     1921 acatggtggt ggcgcgggtg ccgttgaagg tcgcgtcgac ggcagaagac attgcgcgcg
     1981 tggtctgctt cctggcttcc cccgcgtcga gcaacatgac cggcgagctg gtgcgcatgg
     2041 atgccggcat gcatctgatc gtttgattgg gcacatcgta ccggggcgac gctcgcgcgt
     2101 cgtcccggta cgacgcaacc ttgggggcgt tatccgttga aatcgcggat cacgccgctg
     2161 gcgaccagcc ggttccagat gaacagcacc aacagcgcgc cgatggtggc ggttatgaag
     2221 cccgcgccct ggttaggccc gtaatggccg atcgcctggc cgatccatgt cgccaggaac
     2281 gccccggcaa tgccgagcac ggtggtcagg atgaagccgg aggggttgtt cggccccggc
     2341 gagagcatcc gcgcgatgat accggccacg aagcccacaa tgatgaccca gataatgccg
     2401 ctcaattgat gaccctcctt caaacatcac caaagtctcg cgccggcgcg caactcaaag
     2461 ccggtcggac aattc
//
LOCUS       DATLJT010001189         5968 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2384827,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001189 DATLJT010000000
VERSION     DATLJT010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384827"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1250)
                     /locus_tag="VM910_27355"
     CDS             complement(192..1250)
                     /locus_tag="VM910_27355"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI66272.1"
                     /translation="MRHRQCGLREILSALALLVLAPCALAQPSSVAEVASYQGADREQ
                     RLIEGAKREKELTFYSSIPPDDIAALVAAFDKKYGVKVKVWRADSEGFLQRIVGEARA
                     RRFEVDVMAGSSSALEPLYRENLLQEVKSPKLAGIIPQAIAPHRQWVAVYLSTIVQAY
                     NTDLVRKESLPKTYYDLLRPQWKDKLGIEAEDFDWFAQVVMDFGETQGLRLFRDIVAS
                     NGMSVRKGHSLLTNLVAAGEVPLALTVYGFLAEQAKTRGAPLDWFVIPPAIARPTAEG
                     LARNALHPYAAVLFFDFLIGDEGQQILAKRQFVTVSRSIETPFNQGPLQLIDSAMILD
                     QARKWQDLFQKTVIGPSR"
     gene            1483..2757
                     /locus_tag="VM910_27360"
     CDS             1483..2757
                     /locus_tag="VM910_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI66273.1"
                     /translation="MARRVHDKALDSRDARRRLKIRGKPYYRAIERGLHLGYRRLGGG
                     QAGTWVARHYIGGQSYEVQKIGIADDVSDADGAAVLDFWQAQEAAREAMVERAHRAHG
                     KHGPITVGDAMDEYLRFLDGNRKSGADARYRDRALIRPVLGDIEVAKLTADKLRQWMM
                     QLARTPARLRTREGLKQQYAALDTVDAKRARKASANRTLTVLRAALNRAWKDGKVSSD
                     AEWSRVEPFENVNTARIRYLTVAEAKRLINACDPDFRLLVQAALETGARLGELVALEV
                     HDFNSDTGTLAIRQSKSGKSRHVVLTEEGTAFFSQVCAGRPGHATMFVKADGKPWGRG
                     HQGPLMAAACERAKISPSIGFHGLRHTWASLASMAGVPLMVVAKNLGHRDTRMVELHY
                     GHLAPSYVADAIRAGAPRFGFKPDKTVTPMGR"
     gene            3164..3346
                     /locus_tag="VM910_27365"
     CDS             3164..3346
                     /locus_tag="VM910_27365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVI66274.1"
                     /translation="MTDQQVLTIAEYCKREKISRASLYKEWAQGRGPKSYHRGTHRLI
                     SVQAADEYRRQLEEGA"
     gene            3977..5458
                     /locus_tag="VM910_27370"
     CDS             3977..5458
                     /locus_tag="VM910_27370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66275.1"
                     /translation="MNNKDELALHPLVRQMIQEGTRDKPLTLNWAPVERDALDCLKLP
                     DLSPLAMKARKQVIAEALVAGGRFISYSRNRNHYTAQRYYRETHTFRAVIPAVDQLDA
                     EGLIEHEKMMPGSRGLQSRFRPSADLLRAFEKAAVVYKPLETIILRDATGNPVGYLDN
                     RETRQMRRRMTEVNEALTSQKIGLKDRIIREGDRLDNGGRAQAQMHRVFHRCDWDNGG
                     RLYGAFWQNMPPIDGRDQITINGRQTTELDYKGLHIRLLYQEAGKAMPDDPYDIDGWP
                     RQQVKLATLIAVNARTHCSAVRALADALRLDGVSDPFETADRLIRATKARHPDIAWAF
                     GSDAGVRLMRRDSEIALQVMLEMIRATGIAPLPIHDSFIVPIDHEDKLKEAMETAFSC
                     KNTAPKISCKNAPHFRDIVSSGNPSGNLQNDLTIWDGEADGLLPPGMDLAWIAAQRTW
                     LDDLAKRSERFKPDYRDELMIHVANVVKQQLDELDARRAKASQ"
BASE COUNT         1248 a         1762 c         1851 g         1107 t
ORIGIN      
        1 ctcaagcaat tcggctggat tcacgagatc gcgtgcccat ccgagaacga gctctatgtc
       61 gccgagattt tgacctggcg ggtgcagaag ctgatcctgc ggccggacca gcagaaggcc
      121 gcggtcgagt aagcggcaat cggggatcga tcataagcgc cgcaaacgct cctgaccgcc
      181 gacagccgtt gttaccgtga cgggccgata accgtcttct ggaagaggtc ctgccacttt
      241 cgcgcttgat cgaggatcat ggccgaatcg atgagctgga gcgggccctg gttgaagggc
      301 gtctcgatgc tccggctcac ggtgacgaac tggcgcttgg ccaggatttg ctggccctcg
      361 tccccgatca gaaaatcaaa gaacagcacc gccgcatacg ggtgcagcgc gtttcgcgcg
      421 agcccttcgg ccgtggggcg ggcgatcgct ggcggaatca cgaaccagtc gagcggcgcg
      481 cctctggtct tggcctgctc ggccaaaaaa ccgtagaccg tgagcgccaa cggcacttcg
      541 cccgccgcga ccaaattggt aaggaggctg tgacccttgc gcacggacat gccgttcgag
      601 gcgacgatgt cgcggaacag gcgtagccct tgtgtctcgc cgaaatccat caccacctgt
      661 gcgaaccaat cgaagtcctc cgcttcgatg ccaagcttgt ccttccactg cggccgcagc
      721 aggtcgtagt aggtcttggg caggctttcc ttgcggacga gatcggtgtt gtaggcctgc
      781 acgatggtgc tgagatagac cgccacccat tgccggtggg gagcaatggc ctggggaatg
      841 atgcccgcga gcttgggcga tttcacctct tgcagcaggt tctcgcggta gagcggctcg
      901 agcgccgagc tcgacccggc catgacatcg acctcgaagc gccgcgccct ggcctcgccc
      961 acgatgcgtt gcaggaagcc ttccgaatcc gcacgccaga ctttgacctt gacgccatat
     1021 ttcttgtcga aggccgcaac caaagcggca atgtcgtcgg gcggtatgga cgagtagaag
     1081 gtgagctcct tctcccgttt ggcgccttcg atcagccgct gctcgcggtc ggcgccctgg
     1141 tagctcgcga cttcggccac gctgctcggc tgggcgagcg cgcagggagc gagcacaagc
     1201 agcgcaagcg ccgacaggat ttcacgcaga ccgcattggc gatgtcgcat gccgaccgag
     1261 ctccgcgctg cggttggtat cgtccgcaaa ccatggcaca attttgtgcg ccttgtcagg
     1321 gccgaggata cgtccatggc tgtccacgcc tgtttgcggc aacccaataa ccactggcac
     1381 ttctgtgccc tcttgtttgt gcttgtccat ggctgttttc ctctgtatat tggacaaacg
     1441 ttggtcacta tgtcaaccgt gtccaataaa gagggggcca ccatggcacg cagggttcac
     1501 gacaaggcgc tcgatagccg cgatgctcgc cgccggctca agatacgggg aaagccatat
     1561 tacagggcaa tcgagcgcgg gctgcatcta ggataccgcc gtcttggcgg cggacaagct
     1621 ggcacctggg tcgctcgcca ttacatcggc gggcagtcct atgaagtcca gaagatcggc
     1681 attgcggatg acgtgtccga tgccgatggc gcggcggtgc tcgatttctg gcaggcgcaa
     1741 gaggcggcac gggaggcgat ggtcgaacgc gcacaccgcg cccatggcaa gcacgggccg
     1801 atcaccgttg gcgatgcgat ggacgaatac ttgcgcttct tggatggcaa tcggaagtcc
     1861 ggcgctgacg ctcgctatcg ggaccgcgcg ctaatccgtc cggtgcttgg cgatatcgaa
     1921 gttgccaagc tcaccgccga caagctccga caatggatga tgcagttggc gcggacgccc
     1981 gcccggttgc gcacgcgcga ggggctaaag cagcagtacg ccgcgcttga caccgttgac
     2041 gcgaagcgcg cgaggaaggc gtcggcaaac aggacgctaa ccgtcctcag ggccgcgctc
     2101 aaccgcgcat ggaaggacgg caaggtgtca tcggatgcgg aatggtcgcg agtagagccc
     2161 tttgagaacg tcaacaccgc tcgcattcga tacctcaccg ttgcggaggc aaagcggctg
     2221 ataaacgcct gcgatcccga cttccgcttg cttgtgcagg ccgcgctcga aaccggcgcg
     2281 cggctgggcg aactagtggc gttggaagtg catgacttca actccgacac cggcaccctc
     2341 gcgatcaggc aaagcaagtc cggcaaatcc cgccacgtcg tcctaaccga ggagggcaca
     2401 gcgttcttct cccaagtctg cgccggacga cccggacacg ccacgatgtt cgtgaaggcg
     2461 gacggcaaac cttgggggcg agggcatcaa ggtccgctaa tggccgccgc gtgtgagcgg
     2521 gcaaagatta gcccgtccat cggctttcat ggcctgcgcc acacctgggc atctctggcg
     2581 tcaatggctg gcgtgccatt gatggtggtg gcgaagaacc tcggccaccg cgacacccgc
     2641 atggtggagc tgcattacgg ccacctcgcg ccgtcctacg tcgctgacgc catccgcgct
     2701 ggtgcgccga ggttcggctt caagccggac aagacggtca cgccgatggg gcggtgattg
     2761 tcaaaataaa tttggggccc gctccgcacc tgttggcgct gcggagataa ttagatcggc
     2821 cgatgcgaag gtgacgcgac ggtgacagtt tcgcaggcat gagattatga tatgtggtgg
     2881 gctggctgtc cgaggataat cccatggcgg gatcagcatt acagcgcgtg atgctcactt
     2941 actattcgag cgcaaccgac gtttgcgagc gatgacattt gaatcgattc catttgcgcg
     3001 agaataccga caagccgcga tggcgcaagg cgagatatga ggcattgcga tccgcgcctg
     3061 ttgagtggga ttagtgcctc gatgctgtca caaaaccaaa acactgattg cgtgtgtttg
     3121 tgcgcttcgg tggaccgtgg cgcacagcca aggaaagcct catatgactg accagcaggt
     3181 cctcaccatc gcggagtact gcaagcgcga gaagatttcg cgtgcgtctc tgtacaagga
     3241 gtgggctcag ggccgtggcc ccaagtccta tcatcgcggc acccatcgtt tgatcagcgt
     3301 gcaggcggcc gacgagtatc ggcgccagct tgaggagggg gcatgaccga cacggtgagg
     3361 gccccggcgg cggcaaccgc gcagggccct cggacttctg atcggcttgg tggcacgatc
     3421 aatcccaaac ataccgacac cgcgcccccg aatcaagaat ttgcgggtct tcctcggcga
     3481 gctgttcgcc gacatcgcgg cgggcggccc atgagcgcca cctgtcttgc ctgcggctcg
     3541 ccgtttaaac cacaacgctc gacggcgcgc ttctgtggcc cacgctgccg ggtcgccgcg
     3601 cagcgaactc gcaaccgagg aacacctatt agaatggcgg cgacacgccc tggcgtggcc
     3661 gctggcgcgg ttcttagcgt aacagcaccc gttggggtgt ccggggggca aaaaccgcaa
     3721 agcgttacgc taaagcgcaa acccgccaag ctcgacccgc gcatcgtccc cgatccgaag
     3781 tggccaggaa tgtatcggat taggcgacca gatggctcgc tttccgacat ggcgaatttg
     3841 actcgctgtc gtgatgcgct tcgcggtttc gatgaggccg ccgcgtcatg agtgcggccg
     3901 aggagcttta tgagggatat gcccaataag acgctcacgg cggaccgagg ccgagatcga
     3961 cgctatcaag aagcccatga acaacaagga tgagttggcg ctgcacccgc tagtgcggca
     4021 gatgattcag gagggcacgc gcgataaacc gttgacgttg aattgggcgc cagtcgagcg
     4081 cgacgccctc gattgcctga agctgcctga tcttagcccg cttgcaatga aggcccgaaa
     4141 acaggttatc gccgaggcgc tggtcgccgg cggccgcttc atctcttatt cgcgaaatcg
     4201 gaatcactat acagctcagc gctattaccg cgagacccat acgtttcgag ccgtcatccc
     4261 agccgtcgat caactcgacg ccgagggcct gatcgagcat gaaaagatga tgccgggaag
     4321 tcggggtctt cagtcacggt ttcgcccgtc agccgatctt ctaagggcgt ttgagaaagc
     4381 ggccgtcgtc tacaagccac ttgagacgat cattcttcgg gacgcgaccg ggaacccggt
     4441 cggttacctc gataaccgag aaacgcggca gatgcgccgg cgaatgacag aggttaacga
     4501 ggcgctcaca tcgcagaaaa tcggattgaa agataggatt atccgcgagg gcgaccgcct
     4561 cgacaatggt ggccgagcac aagcgcagat gcatcgggtc tttcaccggt gcgattggga
     4621 taacggcggc cggctttacg gcgccttctg gcagaacatg ccgcccatcg atggacgcga
     4681 tcagatcacc atcaacggcc gacagacaac cgaactcgac tacaagggcc tccatattcg
     4741 gctgctctat caagaggccg gcaaggcgat gccggacgat ccgtatgaca tcgacggctg
     4801 gcctcgtcag caggtcaagc tcgccacctt gatagcggtc aacgcccgaa cacattgcag
     4861 cgccgttcgc gcattggccg acgcgctaag actcgacggc gtatccgatc catttgagac
     4921 cgccgaccgg ctcatccgcg ccaccaaagc gcgccacccg gatatagcgt gggcatttgg
     4981 ctccgacgct ggtgtgcgac ttatgcgccg cgacagcgag atcgccctac aagtcatgct
     5041 ggaaatgatc cgcgcaaccg gaattgcgcc gctgccaata catgacagct tcatcgttcc
     5101 aatcgaccac gaagacaagc tgaaggaagc aatggaaacg gcattctctt gtaagaacac
     5161 ggccccaaaa atctcttgta agaacgcacc ccattttcgc gacatcgtaa gctcgggaaa
     5221 tccttcagga aaccttcaaa acgatcttac aatatgggat ggggaggcgg acggcctcct
     5281 gcctcctggg atggacctgg cctggattgc ggcacagcga acgtggctcg acgatctggc
     5341 gaaacgatcg gagcgcttca aaccggatta ccgggacgag ctaatgattc atgtggccaa
     5401 tgtggtgaag caacagctcg acgaactgga tgccaggcga gcaaaggctt cgcagtgatg
     5461 ccactcaatg ggctcgaagg cgcgaacgcg cgaagagcgc ggaatgagtg aggtgttgca
     5521 agtatatcac atccgaggca ctgcccaagt gttgcagtta tgccacatct gttatcgtga
     5581 aatatcaaat ggatagcctg tttttgggaa cggttctaag cctggggcgc aaatcgcccg
     5641 tcaccggact ccaacatgcg gaggagctga gagagatact gaatcaacgc gagggtggag
     5701 gtgctgatgc ggtgcttttg cttcgtccac cgcaaatgtg agaaggcacg caaatctaaa
     5761 aaaacggcgt tgccacaatt ggcgtttgta gcgctcggca gaccatggcg gtggcgactg
     5821 gcggacgagt catcctcctg ggcgccacgt ccggccggca gttgatccca aaaatttgag
     5881 aagggtacgc gccggttggg ggcctcggct tctcgggccg gccgatttgc gttgccgcga
     5941 attgacgcga tgtgccacta gcgacttt
//
LOCUS       DATLJT010001190         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1926062,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001190 DATLJT010000000
VERSION     DATLJT010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926062"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VM910_27375"
     CDS             complement(<1..492)
                     /locus_tag="VM910_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVI66276.1"
                     /translation="MNIGQSSAPQPSGELIAKFRKIVGDKYALTDAVDIVPYVTEERD
                     LFHGRSPLVLRPGSTAEVAEICKLSNAHKIALVPQGGNTGLVGGQTPHNGEVVISLRR
                     MDKIREIDTASNTMTCEAGVILQVAQQRAAEVDRLFPLSLGAEGSCTIGGNLSTNAGG
                     TTAL"
     gene            complement(508..1518)
                     /locus_tag="VM910_27380"
     CDS             complement(508..1518)
                     /locus_tag="VM910_27380"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVI66277.1"
                     /translation="METGLTSPILPAGEAAIREAARALAQGGLVAFPTETVYGLGADA
                     TNSAAIARLYQAKGRPAFNPLISHVGDLAAARRIGRFDAQAEALAEAFWPGPLTLVLP
                     KAPNCAVADLATAGLETIAIRVPAHPVARAILRAFGGAVVAPSANLSGHVSPTTAAHV
                     KSDLEGRIDLIVDGGAVEVGVESTIVGCFDQPMLLRPGGLPREAIERVLGCALVQPPQ
                     ESESDIAQPLAPGMLSSHYAPRTSIRLNAVDVEPGEALLAFGPPLATDGAAAVMNLSA
                     RGDLNEAAANLFGYLRSLDAGAPRRIAVMPIPEHGLGEAINDRLRRAAVGRENRDAEG
                     RT"
     gene            1589..1665
                     /locus_tag="VM910_27385"
     tRNA            1589..1665
                     /locus_tag="VM910_27385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1623..1625,aa:Pro,seq:cgg)
BASE COUNT          377 a          692 c          720 g          422 t
ORIGIN      
        1 gagcgcggtg gtgccgccgg cattggtgga gagatttccg ccgatggtgc agcttccctc
       61 cgcccccagc gacagcggga acagccggtc gacctccgcg gcgcgctgct gggcgacctg
      121 caggatcacg ccggcttcgc aggtcatggt gttggaggcg gtatcgatct cgcggatctt
      181 gtccatccgc cgcaacgaaa tcaccacttc gccattgtgc ggggtctgtc cgccgaccag
      241 gccggtgttg ccgccctgcg gtaccagcgc gattttgtgc gcatttgaga gcttgcagat
      301 ctccgcaact tcggcggtcg agcccggtcg cagcacgagc ggcgagcggc cgtggaagag
      361 gtcgcgctcc tcggtgacgt aagggacgat gtcgaccgca tccgtgagcg catatttgtc
      421 gccgacgatc ttgcggaact tcgcgatcag ctcgcccgag ggctgcggcg cagaagactg
      481 gccaatattc aatgtcgttc gctccaccta cgtccgtccc tcggcgtccc ggttttctcg
      541 tcccacggcc gcgcggcgca gcctgtcgtt gatggcttcg cccaggccgt gctcgggaat
      601 cggcatgacg gcgatccgtc gcggcgcgcc ggcgtcgagc gatcgaagat agccgaaaag
      661 attcgcggcg gcttcattca gatcaccgcg cgccgacaaa ttcatgacag cggctgcgcc
      721 gtcggtcgca agcggaggcc cgaacgcgag caatgcttcg cccggctcga catcgacggc
      781 attgagccgg atgctcgtgc gcggcgcata atgcgaggaa agcatgccag gcgccagcgg
      841 ctgcgcgatg tcgctctcgc tttcctgggg cggctgcacc agggcgcagc cgagcacgcg
      901 ctcgatcgcc tcgcgcggca gtccgcccgg gcgaagcaac atcggctggt cgaaacatcc
      961 gacgatggtg gattcgacgc cgacttcgac cgcgccgccg tcgacgatca ggtcgatgcg
     1021 gccctcgagg tcgcttttga catgggctgc ggtcgtcggc gaaacatggc ccgacaagtt
     1081 cgccgagggc gccaccacag ccccgccgaa tgcgcgcagg atcgcgcggg ccacaggatg
     1141 ggccggcacg cggatggcaa tggtttcgag ccccgcagtc gcaagatccg ccaccgcgca
     1201 attcggcgct ttcggcagca ccagcgtcag cggtcccggc cagaacgctt cggccagcgc
     1261 ttctgcctgc gcgtcgaaac gcccgatcct gcgggcggct gcgaggtcgc cgacatggga
     1321 gatcagcggg ttgaaggcgg gccggccctt ggcctggtag aggcgcgcaa ttgcggccga
     1381 attggtggca tcggccccaa gcccatagac cgtctcggtc gggaacgcga ccagcccgcc
     1441 ttgcgcaagg gcgcgggcgg cctcgcggat ggcggcctcg ccggcaggca aaatcggtga
     1501 tgtcaggccc gtttccactg ggattaaaat ccttcgtttc cgcttcttgc ggtgcgcgaa
     1561 acccgaggct ataagccgcg gtctcagtcg gagcgtggct cagcccggta gagcactgcg
     1621 ttcgggacgc aggggtcgca ggttcgaatc ctgccgctcc gaccattgat ttcaagcact
     1681 tcataattcc aagcttcgga tcgcgcaatg aaatgcgcaa tgatttgatg gccgccgtca
     1741 tcggcttgcg tgggctgcgt gcgataggga tcgagcatcg cagaggttgt gccaacggag
     1801 gcgggtagtt tcagcggttg agactcgtgg gctggcctgc aaccaggcgg caagcgcttc
     1861 gggcgttcgc gccgcagggg ccaagctctc tttctatcga acccgaaaat catcacgctt
     1921 tggcgcgcgc aagattgggt catctcagag attcgagagc ggccgttcgc tgacgactct
     1981 cagcaccttt caaacacata atcgcctgaa taagttgtgt aatcgatttt tttctaaaaa
     2041 aggttgtgaa cgcggttgaa tgggtgcaaa cttcgtcagc tctactcgtc agacatttgc
     2101 actccagagg tctgttgcga tgagttgtat tgccggcaag gccgctcttc tttgtgtcct
     2161 gttttccctg ccgcttcttc cagcattggc gtttgcgcag cagccgcgag c
//
LOCUS       DATLJT010001191         1776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1237004,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001191 DATLJT010000000
VERSION     DATLJT010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237004"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VM910_27390"
     CDS             <1..786
                     /locus_tag="VM910_27390"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVI66278.1"
                     /translation="VHFVPMQPDNVDEQKAMVEQVLKDRPDAVVFIPVDDVAMVEPLR
                     KLNDAKIPVVLAANPLPGNFVTFVGADDFAIGYRQARYLFDHLAGKGRIVILEGMAGA
                     PTNRERLRGYKQALTEAPGITVLASASGSYQQADGKRVMAGFLADHSEIDAVLSANDS
                     MALGALEALKEANRTAVVIGINGILPAVKRIESGEMLATVDFNMFKIGCTATRAAVRY
                     LRREPLPEKVMLPAEVIDKSNYKAWLVPVERRPCPEWDEVVKP"
     gene            complement(916..>1776)
                     /locus_tag="VM910_27395"
     CDS             complement(916..>1776)
                     /locus_tag="VM910_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66279.1"
                     /translation="ARELGMVFQSYAVWPHMTVRQNVIYPLKHRKFSRAEANRKVDEV
                     MDLVGLSEYADRPVVALSGGQMQRVALARSIVYRPQLLLLDEPLSNLDAKLRLRLRDD
                     LRVILKQTGMTALYVTHDQAEAVVLGDRIGVMRDGKLLQLGTPDEIYNRPADLFVANF
                     TGATNELAGTLVARNGKFGVVDFGGGRQGEVALLQPLDPGDKVRIALRPENIAIGRAD
                     RANAFTARLVDRRYQGTQTVYDIDLFGNRLEALELGTAARHAIGIDMPVCIPREVCWA
                     YRDAGPSSYD"
BASE COUNT          325 a          584 c          559 g          308 t
ORIGIN      
        1 gtccatttcg ttccgatgca gcccgacaat gtcgacgaac aaaaagcgat ggtcgagcag
       61 gtgttgaagg accgccccga tgccgtcgtc tttatccctg tcgacgacgt cgcgatggtc
      121 gagccgctca ggaagctcaa cgacgccaag attccggtcg tgctggccgc caatccgctg
      181 ccgggcaatt tcgttacctt tgtcggtgcc gatgatttcg ccatcggcta tcggcaggcg
      241 cgctatctgt tcgatcatct cgcgggcaag ggcaggatcg tcattctgga aggcatggcc
      301 ggcgcgccca ccaaccgtga gcggctgcgt ggctacaaac aggcgctgac cgaggcaccc
      361 ggcatcaccg tgctcgcctc agccagcggc agttaccagc aagccgacgg caagcgcgtg
      421 atggcgggct ttctcgccga tcactcagag atcgatgccg tgctgtcggc caacgacagc
      481 atggcgctcg gcgcgctgga ggcgctgaag gaggcaaatc gcaccgctgt cgtgatcggc
      541 atcaacggca ttctgccggc ggtgaagcgg atcgagagcg gcgagatgct cgcgacggtc
      601 gatttcaaca tgttcaagat cggctgcacc gcgacgcggg cagcggtacg ctatctccgt
      661 cgcgaacccc tgccagagaa ggtgatgctg ccggccgagg tgatcgacaa gagcaactac
      721 aaagcctggc tcgttccggt cgaacggcgc ccgtgcccgg aatgggacga ggtcgtcaag
      781 ccgtaggcag ccttcgccgg cttgttgatc ggactgcagg atgtcgacga ttgcggcccc
      841 cgtggcagcc gtgccgacgc cgacatctct ggcgacgcgg ccctgttcaa tggccgccgc
      901 caccgcgagt tgcggctaat cgtacgacga cggaccggca tcgcggtagg cccaacagac
      961 ctccctggga atgcaaaccg gcatatcgat cccgatcgcg tgccgcgccg cggtgccgag
     1021 ctccagcgct tccagcctgt tgccgaacag gtcgatgtcg taaacggttt gtgtaccctg
     1081 atagcggcga tccacaagtc ttgccgtgaa cgcattggcg cgatccgccc ggccgatcgc
     1141 gatgttttcc ggccgcagcg caatccgcac cttgtcgccc ggatcgagcg gctgcaacaa
     1201 ggccacctcc ccctgtcgcc ccccgccgaa atcgaccacg ccgaacttgc cgttgcgcgc
     1261 caccagcgtg ccggcgagtt cattggttgc gccggtgaaa ttcgcgacga acagatcggc
     1321 cgggcggttg tagatctcgt caggcgtgcc aagctgcaac agcttgccgt cccgcatcac
     1381 gccgatacga tcaccgagca ccacggcttc ggcctggtca tgggtgacgt atagcgcggt
     1441 catgccggtc tgcttgagga tcacacgcag atcgtcgcgc agccggaggc gcagctttgc
     1501 gtcgaggttc gacagcggtt cgtcgagcag aagcaactgc ggccgataga cgatgctgcg
     1561 cgccagcgcc acgcgctgca tctggccgcc cgacagcgcc accaccggcc ggtcggcata
     1621 ttcggacagg cccaccagat ccatcacctc gtcgaccttg cggttggcct cggcacgcga
     1681 gaacttgcga tgcttgagcg gatagatcac gttctggcgc acggtcatgt gcggccacac
     1741 cgcgtaggac tggaacacca tgccgagctc gcgcgc
//
LOCUS       DATLJT010001192         5180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3075347,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001192 DATLJT010000000
VERSION     DATLJT010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075347"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..223
                     /locus_tag="VM910_27400"
     CDS             29..223
                     /locus_tag="VM910_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HVI66280.1"
                     /translation="MDVFLSKFVLPVAVGAVAVVLLLGLVNMMRGGSPNTSQKLMRLR
                     VLLQFVAIVITMLTVWVMGR"
     gene            246..818
                     /locus_tag="VM910_27405"
     CDS             246..818
                     /locus_tag="VM910_27405"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVI66281.1"
                     /translation="MVVLNRIYTRTGDDGTTALGSGERRPKFDLRIAAYGTVDETNAA
                     IGVARLHLHDAREIDAMLGMIQNDLFDLGADLSVPEREGKAERLRVAASQVERLERDI
                     DSLNETLAPLTSFVLPGGTAGAAYLHLARTICRRAERMVVELAARPDEPVGDAAIQYL
                     NRLSDFLFVASRAANSNGADDVLWVPGQNR"
     gene            944..1693
                     /locus_tag="VM910_27410"
     CDS             944..1693
                     /locus_tag="VM910_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVI66282.1"
                     /translation="MKILVPVKRVVDYNVKVRVKSDGSGVELANVKMSMNPFDEIAVE
                     EALRLKEAGKATEVVVVSIGPAQASETLRTGLAMGADRGILVKAEGNVEPLAVAKILK
                     AVADEEKPGLVILGKQAIDDDSNQTGQMLAALLGWPQATFASKLEVDGSDFKVTREVD
                     GGLQTVKLKGPAIVTTDLRLNEPRYASLPNIMKAKKKPIAEKGAADYGVDLTAHLEIL
                     KTAEPPGRKGGVKVKDIAELVSKLKNEAGVL"
     gene            1695..2639
                     /locus_tag="VM910_27415"
     CDS             1695..2639
                     /locus_tag="VM910_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HVI66283.1"
                     /translation="MTTLLIAEHDNASLKDATNKALTAASQLGADVDVLVAGQNAKGA
                     AEAASKLAGVKKVLLADGEAYAHDLAEPLAALIVSLAPSYDAFVAPATSRFKNVMPRV
                     AALLDVMQISEIIKVVSPDTFERPIYAGNAIQTVKSKDAKKVITVRTSTFTAAGAGGS
                     AGIENAAAAADPGLSSFVGEEVAKSDRPELTSAKIIVSGGRAMQSRENFAKYIEPLAD
                     KLGAGVGASRAAVDAGYAPNDWQVGQTGKVVAPELYIAIGISGAIQHLAGMKDSKVIV
                     AINKDEDAPIFQVADYGLVADLYQAVPELTDELGKLGK"
     gene            2738..3616
                     /locus_tag="VM910_27420"
     CDS             2738..3616
                     /locus_tag="VM910_27420"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HVI66284.1"
                     /translation="MTMIKKVGVIGSGQMGNGIAHVAAVAGFDVVLNDVSAERLKSGM
                     ATINGNLTRQVAKKVLTEDAKAKALERITLAETLEGLADCDLVIESAVEKEDIKRRIF
                     QDVCAVLKPEALVASNTSSISITRLAASTDRPERFIGIHFMNPVPLMELVELIRGIAT
                     DDSTFEAAKEFVGKLGKQIAVSEDFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAA
                     MKLGAHHPMGPLELADFIGLDTCLSIMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKT
                     QRGFYDYRGAKPVPTR"
     gene            3697..3897
                     /locus_tag="VM910_27425"
     CDS             3697..3897
                     /locus_tag="VM910_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HVI66285.1"
                     /translation="MDLVSMAASIMAAKAGALQQEVATSTLKSNLDAQKSTVLTLLGA
                     GQQALSLANVGPGVGGNLNISA"
     gene            complement(3911..4585)
                     /locus_tag="VM910_27430"
     CDS             complement(3911..4585)
                     /locus_tag="VM910_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVI66286.1"
                     /translation="MTNDMPQNPSSLPPALRRVPIAIAAVVIGGVVGYAGIHGFGGFK
                     GNSGGDPACRAAVDTARRIEPLARGEVAALTMATTPLRLPDLVFEDADGKPKKLSDWQ
                     GKTVLVNLWATWCVPCRREMPALQGLQEKLGGPNFEVVAINIDTRDPEKPRTFLQEAN
                     LSRLGYFNDQKAKVFQDLKSIGRALGMPTSVLVDPKGCEIATISGPAQWDSDDAIKLI
                     TAAVQP"
     gene            4696..>5180
                     /locus_tag="VM910_27435"
     CDS             4696..>5180
                     /locus_tag="VM910_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HVI66287.1"
                     /translation="MSNKMWGGRFSASPDAIMEEINVSIDVDRHLFAQDIAASKAHAA
                     MLAAQGIITASDAKDIGKGLDAILSEIGKGSFDFKRTLEDIHMNVESRLAELIGPAAG
                     RLHTARSRNDQVATDFRLYVRDVIDETDAALASFQRALVARAVEHAATVMPGFTHLQT
                     A"
BASE COUNT          985 a         1603 c         1677 g          915 t
ORIGIN      
        1 gcgccgggcg gggtgttatg tagccccaat ggatgtcttc ctgagcaagt tcgtacttcc
       61 cgttgcggtc ggcgcggttg ccgtggtgct gctgctcggc ctcgtcaaca tgatgcgcgg
      121 tggttcgccc aacacgtccc agaagctgat gcgcctgcgc gtgctgctgc agttcgtggc
      181 gatcgtgatc accatgctca cggtctgggt gatgggacgc taaggcagtc taaaggagca
      241 aggacatggt cgtcctcaat cgcatctaca cgcggaccgg cgacgacggc accaccgcgc
      301 tcggcagcgg cgagcgtcgc ccgaaattcg atctgcggat cgcagcctat ggaacggtcg
      361 acgagacgaa tgcggcaatc ggcgtggcgc ggttgcattt gcacgacgcg cgcgagatcg
      421 acgcgatgct cgggatgatc cagaacgatc tgttcgatct gggcgccgat ctctcggtgc
      481 ccgagcgcga gggcaaggcg gaacgcctgc gggtcgccgc aagccaggtc gagcgcctgg
      541 aacgcgatat cgacagcctg aatgagacgc ttgcgccctt gacctccttc gtgctgccgg
      601 gcggcacggc aggagcggca tacctgcacc tcgcgcgcac catatgtcgc agagccgaac
      661 ggatggtcgt ggaactcgcc gccaggccgg acgagccggt cggcgacgca gcgattcagt
      721 acctgaatcg cctgtcggat ttcctgtttg tcgccagccg tgccgccaat agcaacggcg
      781 ccgacgacgt gctttgggtc ccgggccaga accgctaaga ttggctcgcg aagtcaaatt
      841 ttggcctccg cgcgcgttga ccggggtgag cggtcgcttt aggttccgcg ccaagccagc
      901 aaaacaagcc aatcaacccc gcaaacttct agcgagaaga tcgatgaaga ttctggtgcc
      961 ggtcaagcga gtggtcgatt acaacgtcaa ggttcgcgtc aagagcgacg ggtcgggcgt
     1021 tgaactcgcc aatgtcaaaa tgtcgatgaa tccgttcgac gagatcgcgg tcgaggaggc
     1081 gctacggctg aaggaagccg gcaaggcgac cgaggtcgtg gtggtctcga tcggtcccgc
     1141 gcaggcctcg gagacgttgc gcaccgggct tgccatgggc gctgaccgcg gcatcctggt
     1201 caaggcggaa ggcaatgtcg aaccgctggc ggtcgcgaag attctcaaag ccgtggccga
     1261 tgaggagaag ccgggtcttg tcattctggg caagcaggcg atcgacgacg actccaacca
     1321 gaccggccag atgctggctg cgctgcttgg ctggccgcaa gccacctttg cctcgaagct
     1381 cgaggtcgac ggctccgatt tcaaggtgac gcgcgaggtc gacggcggcc tgcagaccgt
     1441 caagctgaag gggcccgcaa tcgtcaccac cgacctgcgc ctgaacgagc cgcgctacgc
     1501 gtcgcttccc aacatcatga aggcgaagaa gaagcccatc gccgagaagg gcgccgccga
     1561 ttacggcgtc gatctcaccg ctcatctcga aatcctcaag acagcggagc cgccgggccg
     1621 caagggcggc gtcaaggtaa aggacatcgc cgagctcgtg tcgaaactga agaatgaagc
     1681 cggggttctc tgagatgacc acgctgttga ttgccgaaca cgacaacgcc tcactgaagg
     1741 acgcgaccaa caaggccctg acggctgcaa gccagctcgg tgccgatgtg gacgtgctgg
     1801 tggcgggcca gaacgccaag ggagcggcgg aagccgcctc gaagcttgct ggcgtcaaaa
     1861 aggtactgct cgccgacggc gaggcctacg cccacgacct cgccgaaccg ctcgctgcgc
     1921 tgatcgtgtc gcttgcgccc tcctacgacg cgttcgtggc gcctgcgacc tcccgcttca
     1981 agaacgtgat gccgcgcgtc gccgcgctgc tcgacgtcat gcagatctcc gagatcatca
     2041 aggtggtttc gcccgacacc ttcgagcggc cgatctacgc cggcaacgcc atccagacgg
     2101 tgaagtcgaa ggacgccaag aaggtgatca cggtgcgaac ctcgaccttc acggcagccg
     2161 gagcaggcgg cagcgcggga atcgaaaacg ccgccgctgc ggccgatccg ggcctttcca
     2221 gcttcgtcgg cgaggaggtc gcaaaaagcg atcgtcccga gctgacctcg gcgaagatca
     2281 tcgtttccgg cggccgcgcg atgcaaagcc gggaaaactt cgccaaatat atcgagccgc
     2341 tggccgacaa gctgggcgcc ggcgtcggcg cctcgcgcgc cgcggtcgat gccgggtatg
     2401 cgccgaacga ctggcaggtc ggacaaaccg gcaaggtagt ggcgccggag ctctatattg
     2461 cgattggcat ttccggcgcg atccagcatc ttgccggcat gaaggactcc aaggtgatcg
     2521 tcgcaatcaa caaggacgaa gacgcgccga tcttccaggt tgccgattat ggcctggtcg
     2581 cggacctcta tcaggcggtt cccgaactga ccgacgaact cggcaagctc ggaaaatagc
     2641 ctttaggtgc accggcagga gaggataggc tccggccggt gtcttgtttc atggggatgg
     2701 caggcgccat ctgcgcgccg ctgcggtgga agacaggatg acaatgatca agaaggtcgg
     2761 cgtaatcggc tcgggacaga tgggcaatgg gattgcgcat gtcgcggcag tggcgggatt
     2821 cgacgtcgtg ctcaacgatg tgtctgccga gcgcctgaaa tcgggaatgg cgaccatcaa
     2881 cggcaatttg acgcggcagg ttgccaagaa ggtcctcaca gaggacgcca aggccaaggc
     2941 gctggagcgg atcacgctcg ccgagacgct cgaggggctt gccgactgcg atctggtgat
     3001 cgagagcgca gtcgagaagg aagacatcaa gcggcgcatt ttccaggacg tttgcgccgt
     3061 cttaaagccg gaggcgctgg ttgcctccaa cacctcctcg atctcgatca cccggctcgc
     3121 cgcgagcacc gaccgcccgg aacgcttcat cggcattcat ttcatgaatc cggtgccgct
     3181 gatggagctg gtcgaactga tccgcggcat cgccaccgac gattcgacct tcgaggccgc
     3241 caaggaattt gtcggcaaac tcggcaagca gatcgcggta tccgaggatt ttccggcctt
     3301 tatcgtcaac cgcatcctgc tgccgatgat caacgaggcg atctatacgc tttatgaggg
     3361 ggtcgggaac gtcgaggcga tcgatgcggc gatgaagctc ggcgcgcatc acccgatggg
     3421 accgttggag ctcgccgatt tcatcggtct cgacacctgt ctgtcgatca tgcaggtact
     3481 gcacgagggg ctcgccgact ccaaataccg gccgtgcccg ctcctggtga aatatgtcga
     3541 ggccggctgg ctcgggcgca aaacccagcg cggcttctac gattatcgcg gcgccaagcc
     3601 ggtgccgacc cggtgaggca tttcgccgcc gctctcgcct ccacccctcg ttaaccctcc
     3661 ccgcttaggg tgtccgccgg gcgaggggag tgacgtatgg atctcgtgag tatggcggcc
     3721 agcatcatgg ccgcgaaggc gggcgcgctt caacaagagg tcgcaacctc gaccctgaaa
     3781 tcgaacctcg atgcccagaa atccacggtt ctgaccctgc tcggcgccgg tcagcaggcg
     3841 ctatccctcg ccaatgtcgg ccccggcgtc ggtggcaatc tcaacatcag cgcgtagacg
     3901 tcggcagcct ctagggctgc accgcggccg tgatcagctt gatcgcgtcg tcgctgtccc
     3961 attgtgcggg tccggagatg gttgcgatct cgcagccctt ggggtcgaca agcacggagg
     4021 tgggcatccc gagcgcccgc cctattgatt taagatcctg aaataccttt gctttttggt
     4081 cattgaaata acccagccgg ctcagattgg cctcttgcag gaaagtccgg ggtttttcgg
     4141 ggtcgcgggt gtcgatgttg atcgcgacca cttcgaaatt cggtcccccg agcttctcct
     4201 gcagcccctg caacgccggc atttcgcgcc ggcagggtac gcaccaggtg gcccacaggt
     4261 tgaccagcac ggttttcccc tgccagtccg acagcttttt cggcttgccg tcggcatcct
     4321 cgaagacgag gtcgggcagc ctgagtggag ttgtggccat ggtcagggcc gccacctcgc
     4381 cacgggcgag cggttcgatc cttctcgccg tgtccaccgc cgcgcggcag gcgggatcgc
     4441 cgccgctatt gcccttgaac cccccgaacc cgtgaatccc ggcatagccg acgaccccgc
     4501 cgatcacgac cgccgcgatc gcgatcggta cgcggcgcag cgccggtgga agagacgagg
     4561 ggttttgggg catatcgttt gtcattctgt cccggatacg gttatgcagg ggcctatatt
     4621 acggccggct cagccggcaa agggcggcgc gcgtttgcgc gcgtggatgt gaacggcagg
     4681 cgtgagcacg aggacatgag caacaagatg tggggcgggc ggttttccgc ttcgcccgat
     4741 gcgatcatgg aggaaatcaa cgtctccatc gacgtcgatc ggcacctctt tgcccaggac
     4801 atcgccgctt ccaaggcgca tgccgcgatg ctcgccgcgc aaggcatcat cactgcgagc
     4861 gacgcgaaag acatcggcaa gggcctggac gcgattttgt cggagatcgg caagggctct
     4921 ttcgacttca agcggacgct cgaagacatc cacatgaacg tcgagtcaag gcttgcggag
     4981 ctgatcgggc cggccgcggg gcggttgcac accgcgcgct cgcgcaacga ccaggtagcg
     5041 accgacttcc gtctctatgt ccgcgacgtg atcgacgaga ccgatgcggc cttggcctcc
     5101 ttccagcggg cgctggtcgc gcgcgcggtc gagcatgccg cgaccgtgat gccgggtttt
     5161 acccatctgc agacggcgca
//
LOCUS       DATLJT010001193         7728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1926558,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001193 DATLJT010000000
VERSION     DATLJT010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926558"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /gene="ftsW"
                     /locus_tag="VM910_27440"
     CDS             complement(<1..1101)
                     /gene="ftsW"
                     /locus_tag="VM910_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HVI66288.1"
                     /translation="MLSREERNPLSEWWWTVDRPLLGAILALMLSGIVLSLAASPPVA
                     TRIGLDPFHFFSHHVLFLLPSLLVLIGVSFMSPRQIRRTALVVFAVSVVLIVATLLIG
                     PEVKGSRRWITLLGLNIQASESAKPAFVVIAAWLFAESARRPEMPATSMALVLLLMLV
                     SLLVMEPDFGQTMLILMVWGALFFIAGMRMIWVAGLAGAAGAGLFGAYLFVPHVAGRI
                     KRFMNPASGDTFQVDTAMEAFYNGGWFGLGPGEGIAKRNLPDSHTDFVFAVAAEEFGI
                     ILCLALLALFAFIVIRALSRAYATEDMFARFAASGLAIMFGVQAAINMSVNLQLIPAK
                     GMTLPFISYGGSSIVSLAYGVGMLLALTRLRPR"
     gene            complement(1179..2579)
                     /gene="murD"
                     /locus_tag="VM910_27445"
     CDS             complement(1179..2579)
                     /gene="murD"
                     /locus_tag="VM910_27445"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVI66289.1"
                     /translation="MIPVTSFAGKTVAVFGLGGSGLASCEALKAGGAEVIAGDDSAEN
                     LAKAAQAGFVTADLRSVPWTNFAALILTPGAPLTHPAPHWSVLLAREAGIEVIGDIEL
                     FCRERRRHAPDAPFVAITGTNGKSTTTALIAHLMRFAGYDAQMGGNIGTAILSLEPPR
                     MGRVHVIEMSSYQIDLSPSLDPTVGILLNISEDHIDRHGTLAHYAAVKERLVSGVMPQ
                     GTAIVGVDDIHSRNAADRIEQSGTRVVRVSVRNPLADGVYVEHETILRASGGARSEIA
                     RIGGIGSLRGLHNAQNAACAAACALALGVGREVLQNGLRSFPGLAHRMEQVGRLGQVL
                     FVNDSKGTNADAAAHALSSFADIFWIAGGKPKVGGISGLTEFFPRIRKAYLIGEAAKE
                     FSETLGTRVAHEISETLDVAVANAARDAQASGLADPVVLLSPACASFDQYRNFEIRGT
                     KFRDLVNALPGVKPVV"
     gene            complement(2590..3699)
                     /gene="mraY"
                     /locus_tag="VM910_27450"
     CDS             complement(2590..3699)
                     /gene="mraY"
                     /locus_tag="VM910_27450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HVI66290.1"
                     /translation="MFYWLTELSNTFPPGFGWLRTLLNVFRYITFRTGGAVVTGALFV
                     FLFGPWIIDHLRVRQGKGQPIRSDGPQSHIITKKGTPTMGGLMILSGLVVSTLLWTNP
                     VNPYVWIVLAVTLGFGFVGFYDDYLKVTKQTSSGFAGRIRLVLEAIIALVACYALTKL
                     GRDAASTSLAIPFFKEVIINFGWWFVVFGAFVIVGAGNAVNLTDGLDGLAIVPVMIAS
                     ASFGLIAYLAGNAVFSEYLQINYVAGTGELTVLTGAMLGAGLGFLWFNAPPASIFMGD
                     TGSLALGGMLGAIAVAVKHEIVLAVIGGLFVLEAVSVIVQVASFKLTGKRIFKMAPIH
                     HHFEQLGWTEPQIVIRFWIISVMLALAGLSTLKLR"
     gene            complement(3836..5224)
                     /gene="murF"
                     /locus_tag="VM910_27455"
     CDS             complement(3836..5224)
                     /gene="murF"
                     /locus_tag="VM910_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HVI66291.1"
                     /translation="MSKPLWTIAGVAKALGLSGSFPDTPIDFVTQDSRLVKPGSLFVA
                     LSGTPSGGFVSSFASARDGWQFATNAEAAGAVAMVVPHRIAGVSVPQLVVKDTLLDGL
                     WTLSRAARARFKGPVIGLTGSAGKTSTKEMIAAYPDAYASPSSFNNFWGVPLTLCNAK
                     PDASLLVVEMGMNQTGEIARLSELTRPTVALVVNVQPVHLEKLGSLEAIRREKVSIAQ
                     GLARDGVLVLPARLDAPEWNGKVVRFGADSEVRELSHVAQGEIWRVAAQVAGERIEFG
                     LNPGAPHRLHNALAALASIDAAGLDPQRLAERLETVGIMSGRGVEQSVAGVTVIDDSF
                     NGNPASMVAALDSLTARPIRRGRRIAILGDMLELGSEAPRYHQELAKHLAAIDGIYCV
                     GPLMRHLYDLLPENKGLGWHEDPATLEPQKIASLLEADDVVVVKGSKKMFWVNKFVPG
                     LVAALEAKAPTA"
     gene            complement(5221..6678)
                     /locus_tag="VM910_27460"
     CDS             complement(5221..6678)
                     /locus_tag="VM910_27460"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVI66292.1"
                     /translation="MKLRDLFDDEATIGPQAEAVAISGLAVDSRMVKPGELFFALAGS
                     KTDGARFIDAAIANGAVAVAGDRASQNVLSVPFVSTKNPRRALALAAAKFYARQPATI
                     AAVTGTSGKTSVAAFTRQIWQRLGHASASIGTIGLVSPKRTVYGSLTTPDPIALHRQL
                     DEIAREGVTHLAFEASSHGLDQYRLDGVRISAGGFTNLSRDHLDYHPDVAHYLAAKLR
                     LFRDLIPPGGAAVISADHDCSQEVIEAARARGLRLLTVGGKGDGRGEGICLAEATIDG
                     FAQQLVIEHRGKKFSVRLPLVGAFQIENALVSAGLAIATGSDPDAVFASLEHLEGAKG
                     RLERVGEHNGAPIFVDYAHKPDALAKALQALRPYAGRKLVVVFGAGGDRDVGKRPLMG
                     AIAAENADRVIVTDDNPRSENPENIRAAILAAAKGASEIGDRASAIRTAIDTLESGDA
                     LLIAGKGHETGQIVGDRVLPFSDHDAVAAALARVA"
     gene            complement(6753..>7728)
                     /locus_tag="VM910_27465"
     CDS             complement(6753..>7728)
                     /locus_tag="VM910_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVI66293.1"
                     /translation="KTGEIVAMVSVPDFDPNSPKEAHDPDRINRLTTGVFEMGSTFKA
                     FTLAMALDSGKYDLNSMWDARGPLHYGKFAIHDDEPKGRFLNMKEVFTFSSNVGAARI
                     ALSQGVEAHKAFLRKMGQLERLRTELPESASPLVPRHWGELNTVTIAFGHGIAVAPLQ
                     AVMGIDALVNGGYLIPPTFMKRTEQEAMEIAKRVIKPETSEKMRFLMRLNAEIGTARK
                     ADVAGYYIGGKTGTAEKVVNGHYAKKRVLTAFTAILPADNPKYQLLVMLDEPQPLKET
                     HGFITSGWNAVPTGGNVIARIAPLLGVEPRFDLPPPDRLILAASRTTQ"
BASE COUNT         1474 a         2529 c         2420 g         1305 t
ORIGIN      
        1 tcgcggccgg agccgcgtca gcgccagcaa catgccgacg ccataggcga gcgatacgat
       61 cgacgaacca ccataggaga tgaacggcag cgtcatgccc ttggccggaa tgagctgcag
      121 gttcaccgac atattgatcg ccgcctgcac gccgaacatg atcgcaaggc cggaagctgc
      181 aaaacgcgcg aacatgtctt cggtcgcgta ggcccgcgac agcgcgcgga tcacgatgaa
      241 ggcaaacagc gcaagcaacg ccaggcagag aatgatgccg aactcttcgg ccgcgaccgc
      301 gaacacgaaa tcggtatggc tgtcgggcag gttgcgcttg gcaatgcctt cgccgggtcc
      361 aaggccgaac cagccgccgt tatagaaggc ctccatcgcg gtatcgacct ggaatgtgtc
      421 gccggacgcc gggttcatga agcgcttgat gcgtcccgcc acatgcggca cgaacaggta
      481 ggcgccgaac agccccgctc ccgcggcacc agcgaggccc gcgacccaga tcatccgcat
      541 ccccgctatg aagaacagcg cgccccacac catcaggatc agcatggtct ggccgaaatc
      601 cggctccatc accaggagcg acaccagcat caacaacagc acgagcgcca tcgaggttgc
      661 cggcatttcc ggccgccgcg ccgattccgc aaacagccac gccgcgatca ccacgaacgc
      721 aggctttgcg gattcggagg cctggatgtt gaggccgagc agcgtgatcc agcgccgcga
      781 gcccttgacc tcgggcccga tcaggagcgt cgccacgatc agcaccaccg aaaccgcgaa
      841 aacgaccagc gcagtgcgcc ggatctggcg cggcgacatg aaggagacgc cgatcagcac
      901 caggagggac ggcagcagga acaacacgtg atggctgaag aaatggaacg gatcgaggcc
      961 gatccgggtc gcaaccggcg gactagccgc gagcgacagc acgatgccgc tcagcatcaa
     1021 cgccaggatc gcgccaagca gcggccgatc cacggtccac caccactcgg agagggggtt
     1081 gcgttcttca cgggagagca tgggcgttcc cttcgggggc cttcacccca attggtcgcc
     1141 caggttggtt tccgaagggt taacgaagaa tgtaaaagtc acaccaccgg cttgacgccg
     1201 ggcagcgcat tgacgagatc gcggaacttg gtgccgcgga tctcgaaatt gcgatactgg
     1261 tcgaaggacg cgcaagccgg cgacagcaac accacgggat cggcaaggcc ggacgcttgc
     1321 gcatcgcgcg cggcgttggc gaccgcgacg tcgagggttt cggatatctc gtgcgcgacg
     1381 cgcgtgccaa gcgtttcgga aaattccttg gccgcttcgc cgatcagata ggccttgcga
     1441 atccgtggaa aaaattcggt gaggccggag atgccgccga ctttcggctt tcccccggcg
     1501 atccagaaga tatcggcgaa cgacgacagc gcatgcgcgg cagcatctgc attggtgccc
     1561 ttggagtcgt tgacgaacag cacctggccg aggcgtccga cctgctccat gcgatgcgca
     1621 agaccgggaa agctgcgcag gccgttctgc agaacctctc ggcctacccc gagcgccagc
     1681 gcacaggccg cggcgcaagc cgcattctgc gcgttgtgca ggccgcgcaa cgagccgatg
     1741 ccgccgatcc gcgcgatttc gctgcgcgcg ccgccggacg cgcgcaggat ggtttcatgc
     1801 tcgacataga cgccatcggc gagcggattc ctcacggata cgcgcaccac acgcgtgccc
     1861 gactgctcga tgcggtcggc ggcgttgcga ctatggatgt cgtcgacgcc gacgatcgcc
     1921 gtcccctgcg gcatgacgcc cgacaccagc cgctccttta ccgcggcgta atgcgcgagc
     1981 gtgccgtgac ggtcgatatg gtcttcgctg atattgagca gaatgccgac ggtcggatcg
     2041 agcgacggcg acagatcgat ctggtaggag gacatctcga tcacatgcac gcggcccatc
     2101 cgcggcggtt cgagcgacag gatcgcggtg ccgatattgc cgcccatctg agcgtcgtag
     2161 ccggcaaagc gcatcagatg cgcgatcagc gccgtcgtgg tggatttgcc gttggtgccg
     2221 gtgatcgcga cgaacggcgc gtcgggcgcg tggcggcgcc gctcgcggca aaacaactcg
     2281 atgtcgccga tcacctcgat gcccgcttcg cgcgcgagca gcacgctcca gtgcggcgcc
     2341 ggatgggtca gcggcgcgcc gggggtcagg atcagcgcgg cgaaattcgt ccagggcacg
     2401 ctgcgcagat cggcggtgac gaagcctgcc tgcgcggcct tggcaagatt ctcggcgctg
     2461 tcgtcaccgg caatgacttc ggcgccgccc gctttcaacg cctcgcagct cgcaagtccg
     2521 gagccgccga ggccgaacac cgcaaccgtc ttgcccgcaa atgaggtgac ggggatcatg
     2581 gccgcccggt caacgcagct tcaaggtgga gaggccggcg agcgccagca tcaccgagat
     2641 gatccagaag cggatcacga tctgcggctc cgtccagccg agctgttcga aatgatgatg
     2701 gatcggggcc atcttgaaga tgcgcttgcc cgtcagcttg aaggaggcga cctgcacgat
     2761 cacggagacc gcttccagca cgaacagtcc gccgatcact gcaagcacga tctcgtgctt
     2821 caccgcgacc gcgatcgccc cgagcatgcc gccaagcgcg agcgagccgg tgtcgcccat
     2881 gaaaatcgag gccggcggcg cgttgaacca caggaatcca aggccggcgc cgagcatcgc
     2941 ccccgtcagc accgtaagct cgccggtgcc ggcgacgtaa ttgatctgca gatattcaga
     3001 gaagaccgcg ttgccggcga gataggcgat caggccgaag cttgcggagg cgatcatgac
     3061 cggaacgatc gccagaccgt cgaggccgtc ggtcagattg accgcattgc ccgcgccgac
     3121 aatgacgaag gcaccgaaga cgacgaacca ccagccgaag ttgatgatga cctccttgaa
     3181 gaacgggatt gccagcgacg tcgaggcggc gtcgcggcca agcttggtca gtgcgtagca
     3241 ggccaccagt gcaatgatcg cttccagcac cagacggatt cggccggcaa agccgctgga
     3301 ggtctgcttc gtgaccttca aataatcgtc ataaaagccg acaaagccga agcccaaggt
     3361 caccgcgagc acgatccaga cgtatggatt gaccggattg gtccagagca gcgtcgacac
     3421 caccagtccg gacagaatca tcaacccgcc catggtgggc gtgcctttct tggtgatgat
     3481 gtgggattgc ggaccgtcgc tgcgaatcgg ctggcccttg ccctgtctga cccgcagatg
     3541 atcgatgatc cagggaccga acagaaacac gaacagcgcg ccggtgacga ccgcgcctcc
     3601 ggtgcgaaag gtaatgtagc gaaacacgtt gagaagcgtg cgaagccatc cgaaaccggg
     3661 aggaaaggta ttggagagct cggtcaacca gtaaaacatt cagttccgcc ctgtaccgcg
     3721 ccttgaaaag gcgcggaatt gccgggaaag cgatgtcagt gcgcctcaga ccctttaagc
     3781 atgatcgcag cgcaagcgcg agtggtcatg agcggaaaga tcggcgtccc gcgcatcatg
     3841 ctgtcggcgc cttcgcctcg agcgctgcga ccaatcccgg aacaaattta ttcacccaga
     3901 acattttctt gctgcccttg acgactacca cgtcgtcggc ttccaggagg cttgcgattt
     3961 tctgcggctc gagcgttgcg ggatcctcgt gccaaccgag tcccttgttc tccggcagca
     4021 aatcgtacaa gtgccgcatc agcggaccaa cacaatagat gccgtcgatt gcggccaggt
     4081 gtttcgccaa ttcctggtga tagcgcggcg cctcgctgcc gagttccagc atgtcgccga
     4141 gaatggcgat gcgccgtccg cgcctgatcg gccgcgccgt caggctgtcg agcgccgcca
     4201 ccatgcttgc cggattgccg ttgaaactgt cgtcgatgac ggtcacgccc gcgacgctct
     4261 gctcaacgcc gcgcccgctc atgatcccga ccgtctccag cctctcggca agccgctgcg
     4321 gatcgaggcc cgcggcgtca atcgaggcaa gcgccgcaag cgcattgtgc aggcgatgcg
     4381 gcgcgccggg gttgaggccg aactcgattc tttcacctgc cacttgcgcg gcgacccgcc
     4441 agatctcgcc ctgcgcgacg tggctcaatt cccgcacttc cgagtcagcg ccgaagcgga
     4501 cgactttccc attccattcc ggtgcatcga ggcgggcggg cagcaccagc acgccgtcgc
     4561 gtgccaggcc ttgcgcgatg ctcacctttt cccgccggat cgcctcgaga ctgccgagct
     4621 tttccagatg caccggctgc acattgacca cgagggcgac tgtaggccgc gtcagctcgc
     4681 tcaatcgcgc gatctcgccg gtctggttca tgcccatctc gacgaccagg aggcttgcat
     4741 cgggtttcgc gttgcacagc gtcagtggta cgccccagaa attgttgaag ctgctcgggc
     4801 tggcatatgc gtccggatag gcggcgatca tttccttggt gctggttttg cccgcactgc
     4861 cggtcagacc gatcaccggt cctttgaatc gcgcacgcgc cgcgcggctc aaggtccaga
     4921 ggccgtcgag cagcgtgtcc ttcaccacca gttgcgggac gctcacgccc gcaatccggt
     4981 gcggcacgac catcgccacc gctccggccg cctccgcatt ggtggcaaac tgccagccgt
     5041 cgcgggcgct ggcgaagctc gagacaaatc cgccgctcgg cgttccgctg agcgccacga
     5101 acaggctgcc tggtttcacc agccggctgt cctgcgtgac aaaatcgatt ggcgtgtcgg
     5161 gaaagctgcc cgaaaggccc aatgccttcg ccacccccgc aatcgtccac agcggcttgc
     5221 tcatgcaact ctcgcaagtg cggcggcaac cgcgtcgtga tcgctgaacg gcagcacgcg
     5281 atcgccgacg atctgcccgg tctcgtgtcc cttgcccgcg atcaaaagag cgtcgcccga
     5341 ttccagcgta tcgatcgccg tgcggatcgc gctggcgcgg tcgccaattt cgcttgcgcc
     5401 cttcgcggcg gcgaggatcg cagcacgaat gttttcggga ttctcgctgc gcggattgtc
     5461 gtcggtgacg atgacgcggt cggcgttttc ggcggcgatc gcgcccatca acggacgctt
     5521 gccgacgtcg cggtcgccgc cagcgccaaa caccacgacc agcttgcgcc cggcgtaggg
     5581 ccgcagcgcc tgcagcgcct ttgctagtgc atccggcttg tgcgcgtaat cgacgaagat
     5641 cggcgcgccg ttgtgctcgc cgacccgttc gagccgcccc ttggcacctt cgagatgctc
     5701 gaggcttgcg aacaccgcat ccggatcgct gccggtcgca atggcaagcc ccgccgacac
     5761 cagcgcattc tcgatctgga aggcgcccac cagcggcaac cgcaccgaga actttttgcc
     5821 gcgatgttcg atgacgagct gctgcgcaaa gccgtcgatc gtggcctcgg ccaggcaaat
     5881 gccctcgcct cttccgtcgc ctttgccgcc gacggtcaga agccgcaagc ctcgtgcccg
     5941 cgccgcctcg atcacctcct gcgagcagtc gtgatcggca gagatcaccg ccgcgccacc
     6001 gggcggaatc aaatcgcgga acaacctcag cttggcggcg agataatggg cgacatcggg
     6061 atggtagtcc aagtggtcgc gcgaaagatt ggtgaagccg ccggccgaga tgcgcacgcc
     6121 atccagccga tattgatcca gtccatgaga agaagcttcg aacgccagat gggtcacgcc
     6181 ctcccgtgcg atctcgtcga gctggcgatg cagcgcgatc ggatcgggcg tcgtcagcga
     6241 gccatagacc gtgcgtttcg gcgagaccag tccgatggtc ccgatgctgg cggaggcatg
     6301 gccgagccgc tgccagatct gtcgcgtaaa cgccgcaacc gaggtcttgc cgctggtgcc
     6361 tgtcaccgcg gctatcgtcg cgggttgtcg cgcataaaat ttcgccgcgg cgagcgcgag
     6421 cgcacggcgc ggatttttcg tggagacgaa tggcaccgac aacacatttt gcgacgcgcg
     6481 atcgcctgcc accgcgaccg caccgttggc gatcgccgca tcgatgaagc gcgcgccatc
     6541 ggtcttgctg cctgcgagcg cgaagaacaa ctcgcccggc ttgaccatgc ggctgtccac
     6601 ggcaaggccg ctgatcgcga ccgcctcggc ttgcggcccg atcgtcgcct cgtcatcgaa
     6661 gaggtcgcgc agtttcatct tgttccagtc agaccggcgc accagccttt gcgcagactc
     6721 agcagactcg aaaggccggc ggcgcgtatg gcttactgcg ttgtccggga tgccgcaaga
     6781 atgaggcggt caggcggcgg caggtcgaat cgcggttcga cccccaaaag cggcgcaatg
     6841 cgggcaatca cgttgccgcc ggtcggcacc gcgttccagc cggaagtgat gaaaccgtga
     6901 gtttccttca acggctgcgg ctcgtccagc atgaccagaa gctggtattt gggattgtcg
     6961 gccggcagga tcgcggtgaa cgcggtgagc acgcgcttct tggcatagtg cccgttgacg
     7021 accttctcgg cggtgccggt cttgccgccg atatagtagc cggcgacatc cgccttccgc
     7081 gccgtgccga tctcggcatt gagccgcatc aggaagcgca ttttctccga cgtctcaggc
     7141 ttgatcacgc gcttggcaat ctccatggct tcctgctcgg tgcgcttcat gaaggtcggc
     7201 ggaatcaaat agccgccgtt gaccagcgca tcgatcccca tcacggcctg caggggcgcc
     7261 accgcgatgc cgtgcccgaa ggcgatcgtc accgtgttca actcgcccca atgcctggga
     7321 accagcggcg aggcactctc gggcagttcg gtgcgcagcc gttcgagctg acccatcttg
     7381 cgcaggaacg ccttgtgcgc ttctacgccc tgcgacagcg cgatgcgcgc cgcaccgacg
     7441 ttggaggaga aggtgaagac ttccttcatg ttgaggaagc gtcccttcgg ctcgtcgtca
     7501 tgaatcgcga acttgccgta atgcagggga ccccgcgcat cccacattga gttgagatcg
     7561 tattttccgg aatcgagcgc catcgccagc gtgaaggcct tgaacgtgga gcccatctcg
     7621 aacacgcctg tcgtgaggcg gttgatgcga tcgggatcgt gcgcctcctt cgggctgttg
     7681 ggatcgaagt caggcaccga gaccatggcc acgatctcgc cggtcttg
//
LOCUS       DATLJT010001194         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3994754,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001194 DATLJT010000000
VERSION     DATLJT010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994754"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VM910_27470"
     CDS             <1..904
                     /locus_tag="VM910_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVI66294.1"
                     /translation="AEVTAHTELAEIVLDESGYTEMWQKDRSADAAGRLENLKELVRS
                     MEEFENLSGFLEHIALVMDRDGETEDDAVSLMTLHSAKGLEFDNVFLPGWEEGLFPSQ
                     RTLDEQGRAGLEEERRLAHVGLTRARRRAKLYFATNRRIHGTWTTTIPSRFLDELPPA
                     SVEITESKGGTAWGGAGGYGPSRFDNVESFGSSYATPGWQRAQAKARRNGQARGGFDE
                     EQSPFSRDDFGGFARKKRAAVTIEGELVAKSTGTTSEFSLDDRVFHQKFGYGHVVKID
                     GNKLTIAFDKAGEKKVVDSFVERA"
     gene            998..1441
                     /locus_tag="VM910_27475"
     CDS             998..1441
                     /locus_tag="VM910_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66295.1"
                     /translation="MVRGRSILLVLVLALTSPSLASAQAMRVGSAQNMSFGDAAAMLA
                     QSCGADIDANCRGVSLDANRLRECLSRNTDALSSQCRSDYLRAFDSIQKRIAARVTVA
                     NACTREIVKICNGSTRETSKSVPCLTTAHGVSRNCLQAIDDAGYR"
     gene            1438..2121
                     /locus_tag="VM910_27480"
     CDS             1438..2121
                     /locus_tag="VM910_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVI66296.1"
                     /translation="MMRSLKQSLRDIAAMLTALAAFAAPVAVSRGQTPPSRDDIVNQL
                     SRFETAPELDIAALRQKVLDKSRSRSRNEPEPSKRPPIVPELLNLPTFTVNIDFDNDT
                     PVVRPSSYQTVGRIADALVNSSLLPYSFLIVGHTDSNGRREANVQLSQRRADAIRDSL
                     VNTFKISSKRLQSLGLGEEQFVDQAHPTSPANLQIQIVTIAKLPEQSEQPTQPASASK
                     STKKSAKKR"
     gene            2244..>2792
                     /locus_tag="VM910_27485"
     CDS             2244..>2792
                     /locus_tag="VM910_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66297.1"
                     /translation="MPSIISVANVSKTYGSGFKALKNVNLEIGRGEIFALLGPNGAGK
                     TTLISIICGIANLSEGRVMVAGHDISEDYRAARELIGLVPQELHTDAFETVWATTSFS
                     RGLFGKPKNPAHIEKVLKDLSLWDKKDSKIVTLSGGMKRRVMIAKALSHEPQILFLDE
                     PTAGVDVELRKGMWEVVRALRAS"
BASE COUNT          536 a          919 c          865 g          472 t
ORIGIN      
        1 ggccgaagtc accgcgcata ccgagctcgc cgaaatcgtg ctggacgaga gcggctatac
       61 cgagatgtgg cagaaggacc gctccgccga cgccgcaggc cggctggaaa acctgaagga
      121 gctggtgcgc tcgatggagg agttcgagaa cctctcgggc ttcctcgagc atatcgcgct
      181 ggtgatggat cgcgacggcg agaccgagga cgatgcggtg tcgctgatga ccctgcattc
      241 ggccaagggg ctggaattcg acaacgtgtt tctgccgggt tgggaggaag ggctgttccc
      301 gagccagcgc acgctcgacg agcagggccg cgccggcctc gaggaagagc gacgcctggc
      361 ccatgtcggc ctgacgcggg cccgccgccg cgcgaagctt tactttgcga ccaaccgtcg
      421 catccacggc acctggacca ccacgatccc ctcgcgtttc ctcgacgaac tgccgccggc
      481 aagcgtggag atcaccgaat ccaagggcgg cacggcctgg ggcggtgccg gtggctacgg
      541 cccgtcgcgc ttcgacaacg tcgaatcctt cggttcgagc tacgcgacgc cgggctggca
      601 gcgcgcgcaa gccaaggccc gccgaaacgg ccaggctcgc ggcggcttcg atgaggagca
      661 atcgccgttt tcacgcgacg acttcggcgg ctttgcgcgc aagaagcgag ctgccgtcac
      721 gatcgagggg gagctggtcg ccaaatcgac cggcaccact tccgagtttt cactcgacga
      781 ccgcgtgttc caccagaaat tcggctacgg ccatgtcgtg aagatcgacg gcaacaaact
      841 caccatcgcc ttcgacaagg ccggcgagaa gaaggtggtc gacagttttg tggagcgcgc
      901 gtgagcggcc ttaaggcatt cgcgcgcctc ctcagcccgc aaatctcacc cccgccggcc
      961 cttgaccatt gcacgcctca tcgtatgaga tgacgtcatg gttcggggcc ggtcgatcct
     1021 ccttgttctt gtccttgcgc tcacctcgcc gtcgctggcg tcggcgcagg ccatgcgcgt
     1081 tggctcggcg caaaacatga gcttcggcga cgcggcggcg atgctggccc agagctgcgg
     1141 cgccgacatc gacgccaatt gccgcggcgt cagtctcgat gccaatcgcc tgagagagtg
     1201 tttgtcgcgc aacaccgatg cgctgtcgtc acagtgccgg agcgattatc tgcgggcctt
     1261 cgattcgatc cagaagcgca ttgcggcccg cgtcacggtc gccaatgcct gcacgcgcga
     1321 aatcgtcaag atatgcaacg gctcgaccag ggagaccagc aaatcggtgc cgtgcctgac
     1381 gaccgcccat ggcgtgagca ggaactgcct gcaggccatc gatgacgcag ggtatcgatg
     1441 atgcgctcgc tcaagcaaag cctgcgcgac attgcggcga tgctgaccgc ccttgcggcg
     1501 ttcgcggcac ccgttgccgt ctcgcgcggc cagacgccgc cgagccggga cgacatcgtg
     1561 aaccagctca gccgtttcga gacggcgccc gagctcgaca tcgccgcgct gcgccagaag
     1621 gtgctcgaca aatcgcggtc gagatcgcga aacgaacccg aaccctcgaa gcggccgccg
     1681 atcgtgccgg agcttctcaa tctgccgacc ttcaccgtca acatcgattt cgacaacgat
     1741 acgccggtgg tccggccgtc atcctatcag acggtcggcc gcatcgccga cgcgctcgtc
     1801 aattcgagtc tcctgcccta cagcttcctg atcgtcggcc ataccgattc gaatggacgg
     1861 cgcgaagcca atgtgcaact gagccagcgc cgcgccgatg cgatccgcga ctcgctcgtc
     1921 aacaccttta agatctcgtc gaagcggctt cagtcgcttg ggctcggcga ggagcagttc
     1981 gtcgaccagg cgcaccccac atcgccggcc aatctgcaga ttcaaatcgt gacgattgca
     2041 aaattgcccg agcagagcga gcagccgacg cagccggcca gtgcctcgaa gtccacgaag
     2101 aagagcgcga aaaaaagata attgccggtt ttcccggcca attgcgcaag ccctttcaaa
     2161 ttcctatctc tctatggccg atccttttct gcctggcgcc gccgcgcgtg ggcagtgttc
     2221 ccgccacgcc acccaatccc acgatgccct ccatcatctc agttgccaac gtgtcgaaga
     2281 cctatggctc cggcttcaag gcgctgaaga acgtcaatct cgagatcggg cgcggcgaaa
     2341 ttttcgcgct gctcgggccg aacggcgccg gcaagaccac gctcatcagc atcatttgcg
     2401 gcatcgccaa tctcagcgaa ggccgcgtca tggtcgccgg tcacgacatc tctgaagact
     2461 atcgcgccgc gcgcgaactg atcgggctcg tgccgcagga gctgcacacc gacgccttcg
     2521 agacggtgtg ggcgaccacg agcttcagcc gcggcctgtt cggcaagccg aaaaacccgg
     2581 cccatatcga gaaggtgctg aaagacctgt cgctgtggga caagaaggac tccaagatcg
     2641 tcacgctctc gggcggcatg aagcgccgcg tgatgatcgc gaaagcgctg tcgcacgagc
     2701 cgcagatcct gtttctcgac gagccgaccg cgggcgtcga cgtcgaattg cgcaagggaa
     2761 tgtgggaggt ggtgcgggcg ctgcgggcct cc
//
LOCUS       DATLJT010001195         3756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4454983,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001195 DATLJT010000000
VERSION     DATLJT010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4454983"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VM910_27490"
     CDS             <1..582
                     /locus_tag="VM910_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HVI66298.1"
                     /translation="LLGEEQSGHIKEVGFELYQSMLEEAIINLKAGIAEPPPERWSPQ
                     ITVGMPVLIPEDYVADLPVRLSLYRRLADLDTEEEIDNFGAELRDRFGVLPDEVRYLF
                     KVAAIKSYCRRANVEKVDAGPKGAVITFRDNKFAQPDRLVSFIRQHGQAARVRPDMKV
                     VFFQDWETPEERLKGASQILRQLAHLAEEKKAA"
     gene            complement(595..1827)
                     /locus_tag="VM910_27495"
     CDS             complement(595..1827)
                     /locus_tag="VM910_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVI66299.1"
                     /translation="MSQQGPLLIVADDGRPSFATALDEAQMFPLIDTNFANAARAVAQ
                     VQPAAVLADMRGADDERFAALAKLIAAHKPYLPLIAIQPEGIPGENAIPFTPHASNFD
                     RLPARLRAALRVRTLHTTLLRRLTDARAEGSVLPAIDPARDATVLLLGRGGAYPALSV
                     SLGERMGVVGAFSIEAAASHLNNRDIDGIVLGEGFTARVMDAFLTVMSEDARFRHLPI
                     ILTSQELAPTYELPNLEIICGEPDHIAANALPLIRQRAFEAQLGRTLRAFEAKGLLDP
                     QTGLLTLTAFDRDLAGAVKRTQSDGGGLSVARFAFDPQNPRARFDGARIISKLMRKMD
                     FGAVQRDGSVIVAFADTDLRTAHGIARRLFSVMRQTSNGPRATRSDPVVTVATLLPKD
                     SLQSLLARLHAEAHRAAS"
     gene            complement(1922..3427)
                     /locus_tag="VM910_27500"
     CDS             complement(1922..3427)
                     /locus_tag="VM910_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVI66300.1"
                     /translation="MNQTISPTLDTLFQRCLSRKPEAIALIDPNNKPRITGSPPRGLT
                     YAQADRAISALAAHFIEAGLPPHSVLAVQLPNTIEFALTVLAAYRAGLVVALFPLLWR
                     QAELVAALNRTSARAIVTSAKIDGVIHADLAMNAAAEAFSIRHVCGFGSDLPEGMASL
                     DQALAQPSRATRQVVQDGRKAAIISFDVTANGFRVVPRTHLSLIAGGLTLSLESDVPQ
                     GATVMSAFAPCSFAGLAASVVVWLLSGGALALHHPFDGDVLEQQIIECACDTLVAPAP
                     LALRLDELDLATRLPSLRNVIGLWRAPEQVASSPSWTAQNVRMTDAYLFGEAGLFGAR
                     RTTPDGAPAPIKVGPHSAPRELPGSSIAGEILLTSKGTLGLRGPMVAVAAYAPPLPPG
                     EHQLTAQAPRDYVDTEYAARVDRASGEIAITAPPSGVMAVGGYRFLASDLQEWGKRLG
                     QGALLTALPDRLSGHRLAGKAQDNARAREALLQLGLNPLMVEAFRDRTSAA"
     gene            complement(3447..>3756)
                     /locus_tag="VM910_27505"
     CDS             complement(3447..>3756)
                     /locus_tag="VM910_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66301.1"
                     /translation="EQAFYFGSAAADVQGTRNYMGARDPAIDAMIAALLSARDHADFV
                     SAVRALDRVLMSGFYAIPVLNAGEQWIARWNRIERPSVTPLTGYLPESWWQKPGAATK
                     "
BASE COUNT          677 a         1205 c         1255 g          619 t
ORIGIN      
        1 ctcctcggcg aggagcagtc gggacatatc aaggaggtcg gcttcgagct ttatcaatcg
       61 atgctggagg aggcgatcat taacctcaag gccggcatcg ccgaaccacc tcccgagcgc
      121 tggtcgccgc agatcacggt cggcatgccc gtgctgattc cggaggatta tgtcgccgac
      181 cttccggtac ggctgtcgct gtaccggcgg ctggccgacc tcgacaccga ggaggagatc
      241 gacaatttcg gcgccgaact gcgcgatcgc ttcggagtgc tgccggacga agtgcgctat
      301 ctcttcaagg tcgctgcaat caagagctat tgccgcaggg ccaatgtcga gaaggtcgat
      361 gccgggccga agggtgccgt gatcactttc cgcgacaaca agttcgccca gccggatcgg
      421 ctcgtctcct tcatccgcca gcacggccag gctgcgcggg tgcgtcccga catgaaggtc
      481 gtgttcttcc aggattggga gacgccggag gaacggctga aaggcgcaag ccagatcctg
      541 cgtcagcttg ctcacctcgc cgaggagaag aaggccgcgt aggcctctgt gtcgtcagga
      601 cgccgcgcgg tgcgcttcgg cgtggagccg cgccagcagc gattgcaagg aatccttcgg
      661 cagcaaggtc gcgaccgtca cgaccggatc ggagcgcgtg gcgcgcggtc cgttggaggt
      721 ctgccgcatc acgctgaaca gtcgtcgcgc aataccgtgg gccgtccgca gatcggtatc
      781 ggcaaacgcg acgatcacgg agccgtcacg ctggacggcg ccaaaatcca tcttccgcat
      841 caacttgctg atgatccgcg cgccgtcgaa ccgggcacgc gggttttgcg gatcgaaggc
      901 gaagcgggca accgacaacc cgcccccgtc ggactgcgtg cgcttcacgg caccggccaa
      961 atcgcgatcg aacgccgtta gcgtgagaag gcctgtttgc gggtcgagca gccccttggc
     1021 ctcgaacgcg cgcagggtgc ggccaagctg ggcttcgaag gcccgctgcc ggatcagcgg
     1081 caacgcgttg gcggcgatat gatctggctc cccgcagatg atctccaggt tgggcagttc
     1141 gtaggtcggc gccagctcct gtgatgtcag gatgatcggc aaatggcgga agcgcgcgtc
     1201 ttccgacatg accgtgagaa aggcatccat cacgcgcgcg gtgaagcctt cgccgagcac
     1261 gatgccgtca atatcgcggt tgttgagatg gctggcggcc gcttcgatgc tgaaggcgcc
     1321 gacgacgccc atgcgctcgc cgagcgagac cgacagcgcg gggtacgcgc cgccgcggcc
     1381 gagcaggagc accgtcgcat cgcgcgcggg atcaatcgca ggcaaaacgc ttccctccgc
     1441 tcgcgcgtcg gtgaggcgtc gcagcagggt cgtgtgcagg gtgcgcaccc gcagcgcggc
     1501 gcgcagccgc gccggcagac gatcgaaatt gctcgcgtgc ggcgtaaagg gaatggcgtt
     1561 ttcgccagga atgccctcgg gctggattgc aatcaggggc agatagggct tgtgcgccgc
     1621 gatcagcttc gccagtgccg caaaacgctc gtcgtccgcc ccacgcatat cggcgagaac
     1681 cgccgcgggc tgcacttgtg ccacagcccg cgcggcgttg gcgaagttcg tatcgatcaa
     1741 gggaaacatc tgcgcttcgt cgagcgcggt cgcaaatgat ggccgcccgt cgtctgcaac
     1801 gatgaggagc ggaccttgct gggacattgg acaaaccggc gcaatcgaag gaggctacaa
     1861 cggtaatttg ccggtcttaa tgggccgtca acatgagccg tcaacgccgc gcttcgctag
     1921 gtcaggcggc gcttgtccga tcccgaaacg cctccaccat caaggggttg aggccaagtt
     1981 gcagcaatgc ctcccgggcg cgcgcgttat cttgggcttt tcccgccaga cgatgaccgc
     2041 tcaagcgatc cggcaacgcc gtcagcagcg cgccctggcc gagacgtttg ccccattcct
     2101 gcaaatcgga ggcgagaaaa cggtagccgc cgaccgccat gacgcccgag ggcggcgccg
     2161 tgattgcaat ctccccgctc gcgcgatcga ccctggccgc gtattcggta tcgacgtaat
     2221 cgcgcggcgc ctgtgccgtt agctgatgct cgccgggcgg cagcggaggt gcgtaggctg
     2281 caaccgccac catgggtccg cgcaggccga gcgtgccctt ggacgtcagc aggatttccc
     2341 cggcgatgga agaacccggc agttcgcgcg gggcgctgtg cggtccgacc ttgatcggtg
     2401 ccggagcgcc atcgggcgtg gtgcggcggg cgccgaacag cccggcttcg ccgaacagat
     2461 aggcatccgt catacgcacg ttttgcgccg tccaggaagg gctcgacgcc acttgctcgg
     2521 gtgcacgcca caggccgatg acattgcgca ggctcggcag ccgcgttgca aggtcgagct
     2581 cgtccagccg caacgccagt ggcgcgggcg caaccagagt gtcgcaggca cactcgatga
     2641 tctgctgctc gagcacgtca ccgtcgaagg gatggtgcag cgcgagcgcg ccgcccgaca
     2701 acagccagac cacaaccgac gcggcgagac cggcgaaaga gcagggggca aaggccgaca
     2761 tcacggttgc gccctgcggc acatcgctct cgagcgacag cgtcaagccg ccggcgatga
     2821 ggctcagatg cgtgcggggc accacacgaa agccgttggc cgtcacgtcg aaggagatga
     2881 ttgcggcctt gcgaccatcc tggaccactt gacgcgtcgc gcgcgagggc tgcgccagcg
     2941 cctggtcaag cgaggccatg ccctccggca ggtcgctgcc gaagccgcag acatggcgga
     3001 tcgagaacgc ctccgccgcc gcgttcatgg caagatcggc gtgaatgact ccgtcgatct
     3061 tcgcagatgt tacaatggcc cgcgcgctgg tgcggttcag tgctgcgacc agttcggcct
     3121 gccgccacaa caaggggaac agcgcgacca cgaggcctgc gcgatacgcg gccagaaccg
     3181 tcagcgcaaa ttcgatcgtg ttcggcagtt gcaccgcgag caccgaatgt gggggcagtc
     3241 ctgcctcgat gaaatgtgcc gccaatgcgg aaatcgcgcg gtcggcctgc gcgtaagtca
     3301 gccccctggg cgggctgccg gtgatacgtg gcttgttgtt tggatcgatc agcgcgatcg
     3361 cctccggctt gcgcgaaagg caccgctgaa acagtgtgtc gagcgtcggc gatatcgttt
     3421 ggttcacctg ctaccccgtc tccggatcac ttggtcgccg cgcccggttt ttgccaccag
     3481 ctttccggca ggtatcccgt caagggggtg actgatggcc gttctatccg attccagcgc
     3541 gcaatccatt gctcgccggc gttaagcacg ggaattgcgt aaaagccgga catcagcacg
     3601 cgatccagcg cccgcaccgc cgaaacgaaa tcggcatggt cgcgcgcgct cagcagcgcc
     3661 gcgatcatcg cgtcgatggc ggggtccctg gcgcccatgt agttgcgcgt gccctgcacg
     3721 tcggcggcgg cgcttccgaa gtagaaggcc tgttcg
//
LOCUS       DATLJT010001196         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_549862,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001196 DATLJT010000000
VERSION     DATLJT010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549862"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1206
                     /locus_tag="VM910_27510"
     CDS             220..1206
                     /locus_tag="VM910_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66302.1"
                     /translation="MNRPHDRTVACWAFALVALLLMPDRAQALDNVTLITDFGYNGRH
                     AYFFVAIEKGYYKDADLDVKVVRGQGSVDAIRQVGANNAIVGFADAGSLVLARGNDQI
                     PVKLVSIVYAKPPQAVFCREDSGIKQPKDLEGASIANPAGGATVDMFPVYAKAAGFDA
                     SKVKWVVAGSDSLPGLLASNRVPCVGQFTVGEPLLRMQAAPAKLVRFAFADAGVSFYG
                     NGLIATDSTIATKPDIVRRFVAATIRGMKDAFANPHEAGRIMNKYHPAIDVAIAEGET
                     KAVAELAQVKGGELGLIDPARIEETINVINSVFKLKAPVTVNDVYAPGFVSK"
     gene            1246..>2141
                     /locus_tag="VM910_27515"
     CDS             1246..>2141
                     /locus_tag="VM910_27515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66303.1"
                     /translation="MSVARIAVPDFVSNSYFPAIAAIELGFFTREGLDVAHELIFPNY
                     KAYEAMRDGKIDFVAGPAHAVFRAFPDWEGAKLLAALAQGMYWLLVMRTELAATPGDV
                     NSVKGRTIGAAPLVDEGLKRLLIDAGLDLDRDGVHIVGVPGASEPGVSFGVAAAKALE
                     DGKLDGFWANAMGAETAVRRGVGKVVLDVRRGLGPRAAFHYTMSVLATSDHAIKRDPN
                     MVAAGVRAVVKTQQALKRDPSLATRVGKALFPPVEAALIADVIARDLPYYDPTISEAA
                     VDGLSRFAQASGLARRSVSYNQV"
BASE COUNT          400 a          625 c          689 g          427 t
ORIGIN      
        1 tagccggtat acgtaccggc gcgaatctgg cgccgcactg cgcgtggatc cgaacggtcc
       61 cagcgctcgt cccgcaagtc acggccccga tgttcgcccg agataagatc gaccaatgag
      121 ggtccccatt gttgcgacgt gcgcgcgcgg tggattctct ataccaaaca ggactcgagc
      181 ttgtatactg atccagccat tgaggagaga ggttgcgcaa tgaacaggcc ccatgatcgt
      241 acggttgcct gctgggcatt cgctcttgtt gctctcttgc tgatgcctga ccgtgcgcag
      301 gccttggata atgtcacgct gatcaccgac ttcggttaca acggccgcca cgcctatttc
      361 ttcgtcgcga tcgaaaaggg ctactacaag gacgccgatc tggatgtgaa ggtcgtccgc
      421 ggccagggat cggttgatgc catccggcag gtcggcgcca ataatgcgat cgtgggtttt
      481 gccgatgccg gctcgctcgt gcttgcgcgc ggcaacgatc aaatcccggt caagctcgtt
      541 tcgatcgtat atgccaagcc accgcaggcg gtgttctgcc gggaggactc cggtattaag
      601 cagcccaagg atctcgaggg agcctcgata gccaatcccg cagggggcgc aacggtcgac
      661 atgttcccgg tctacgccaa ggccgctggc ttcgatgcga gcaaggtgaa gtgggtcgtc
      721 gcaggcagtg attcgctgcc gggattgctg gcaagcaatc gcgtgccgtg tgtcggtcag
      781 ttcacggtcg gcgagccgct tctgcgcatg caagccgcgc cggccaagct cgtccgtttc
      841 gcgtttgccg atgccggtgt gagcttctac ggcaacggcc tcattgcgac cgacagcacc
      901 atagcgacga agcccgacat tgtgcggcgg ttcgtcgccg ccacgattcg cggcatgaag
      961 gatgcctttg caaatccgca cgaggcgggt cggatcatga acaaatatca tcccgcgatc
     1021 gatgtcgcca ttgccgaggg agaaaccaag gcagtggccg aattggcgca agtcaaaggc
     1081 ggggaactcg ggctgatcga tcccgcccgc atcgaagaaa ccatcaacgt catcaatagc
     1141 gtattcaaac tcaaggcgcc ggtgacggtg aacgacgtct atgcgccagg attcgtatcg
     1201 aagtgacgtg gctgccaggc ccgcatcgac gccccaggag ttcttgtgag cgttgcacga
     1261 atagcggtcc ccgatttcgt ctcaaactcg tactttccgg cgatcgctgc gatcgagctc
     1321 ggcttcttca cacgcgaagg acttgatgtc gcgcatgagt tgatcttccc gaactataag
     1381 gcctacgagg cgatgcgcga cggcaagatc gattttgttg caggtccggc gcacgctgtc
     1441 tttcgcgctt tcccggattg ggagggagcg aagctgctcg ccgcgctggc gcagggaatg
     1501 tactggcttc tggttatgcg gacggagctc gcggcaacgc cgggtgatgt caactccgtc
     1561 aagggccgca cgattggggc ggcgccgctg gtggacgagg ggttgaaacg cctcttgatc
     1621 gacgcaggac tcgatctcga tcgcgacggc gtacacatcg tcggcgttcc tggcgcgagc
     1681 gagccgggcg tctcgttcgg ggtggccgcg gcgaaagcgc tggaggatgg caaacttgat
     1741 ggattttggg cgaacgccat gggggccgag accgccgtgc gccgcggcgt tggcaaagtc
     1801 gtccttgacg tgcggcgcgg attgggtcca cgcgcggctt tccactatac gatgtcagtc
     1861 ttggcgacga gcgaccatgc gatcaagcgc gatccgaaca tggtcgcggc cggtgtgcgt
     1921 gccgtggtga agactcagca ggctttgaaa cgcgatccca gccttgccac acgggtcggc
     1981 aaggcgttgt ttccgccagt agaggccgcg ctgatcgcgg acgtgattgc gcgcgatctg
     2041 ccctactacg acccgaccat ctccgaagcg gcggtcgacg gcctcagtcg ctttgcccag
     2101 gcaagcggtt tggcacggag atcagtgtct tacaatcagg t
//
LOCUS       DATLJT010001197         4830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4223818,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001197 DATLJT010000000
VERSION     DATLJT010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4223818"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VM910_27520"
     CDS             <1..1182
                     /locus_tag="VM910_27520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HVI66304.1"
                     /translation="VVLLDPDRAFPGWWALLPVAGSVLLLSAPQAWGCRRLLSNPTMV
                     WFGLISYPLYLWHWPLLVFFTMIKFNKLTSIERPLVVGLSIALAWATYRYIEVPIRFG
                     RPTRVRTLGVCAGMVLVALAGAIVVRGDGFDFRMPAEIRAMASVPTQAAKWRVHECLL
                     DLKHETTFADDCVDANRRPLVFVWGDSTAGALIPGLREAQQRRQFGIAQFTGSSCPPV
                     LDTDFAFQPNCRAMNDKVFALASQLRPEIVLLHGIHEMFLDAAAETVTRLKRETGARV
                     VVLGAEPQWNRGLPNEVLRHYMLYHRLIPARTFDSVLPNWQDAMFRAKLVPRGAEFIS
                     AHDTMCNAEGCLALIGDRASDISTSDVIHLTEKGSTFLIGAIIDQVLDGSAERRASAL
                     H"
     gene            complement(1192..2328)
                     /gene="recF"
                     /locus_tag="VM910_27525"
     CDS             complement(1192..2328)
                     /gene="recF"
                     /locus_tag="VM910_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HVI66305.1"
                     /translation="MTPSRIHRLTLTHFRNYRTAHFETRGDMVALVGPNGAGKTNCLE
                     AISFLSPGRGLRRATLEDVADNQGDGSWAVAAEIEGALGLATLGTGIDAPQGDASATR
                     RCRIDRETVGSAAAFGDHLRMVWLTPAMDGLFLGAASERRRFFDRLVLAIDSEHSSRV
                     SALDRSLRSRNRLLEVRNFDDHWCDAIERETAELAVAVAAQRGQTAVKLASRLRARGE
                     ASAFPSAEIALDGWMENALLSEPATAVEDRYRKILRDNRARDAAAGRTLDGPHLTDLE
                     VIYAPKGMPARDASTGEQKALLIGLVLAHAALVAETTGITPLLLLDEVVAHLDPNRRA
                     ALFAELQKLGAQIWLTGADPAAFTEIGASSEIFNVENGRIARRA"
     gene            complement(2469..3587)
                     /gene="dnaN"
                     /locus_tag="VM910_27530"
     CDS             complement(2469..3587)
                     /gene="dnaN"
                     /locus_tag="VM910_27530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVI66306.1"
                     /translation="MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLLRADNAQLSL
                     KATDLDLEVTETLAAETATGGATTVPAHMFYDIVRKLPDGAQILLEGDGDRAVLAIRA
                     GRSRFTLQTLPESDFPDLAAGEMTHSFTLTAADVKRLIDRTQFAISTEETRYYLNGIY
                     LHSAGSAKAASLRAVATDGHRLAQVDLALPKGAVGMPGVIVPRKTVGEVQRLIEDNEA
                     EVAVELSAGKIRFTIGNVVLTSKLIDGTFPDYGRVIPQNNDKELVVDKNDFAAAVDRV
                     STISSERGRAVKLALTAGKLVLSVTNPDSGSATEEIEAEYAADPLDIGFNSRYLLDIA
                     AQIEGEAAVLKLADPGSPTLIQDRDNKTALYVLMPMRV"
     gene            complement(3916..>4830)
                     /gene="dnaA"
                     /locus_tag="VM910_27535"
     CDS             complement(3916..>4830)
                     /gene="dnaA"
                     /locus_tag="VM910_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVI66307.1"
                     /translation="LYIHAGVGLGKTHLLQAVTWAGNAGSERKVLYLTAEKFMYGFVA
                     ALKTQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRP
                     PSDLESLDDRVRSRLAGGLVVEMASLGEELRLGILKSRVAAARAHHASFDVPDQVLDY
                     LARTITHNGRDLEGAINRLLAHSKLNNQPVTLDMAEREVRDLIRPQEPKRIKIEDIQR
                     VVARQYNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLH
                     AVRKIEALVAKDVALSEEVESLKRQLQE"
BASE COUNT          796 a         1545 c         1598 g          891 t
ORIGIN      
        1 gttgtgcttc tcgatccgga tcgtgccttt cccggctggt gggcgctgtt gccggtcgcc
       61 ggcagcgtgt tgcttctctc cgcgccgcag gcctggggtt gccgccgcct gctgtcgaac
      121 ccgaccatgg tctggttcgg cctgatcagc tatccgctct atctctggca ctggccgctg
      181 ctggtgttct tcacgatgat caagttcaac aagctcacgt cgattgagcg gcccttggtc
      241 gtcgggttga gcattgcgct cgcctgggcc acctatcgct acatcgaagt gccgatccgc
      301 ttcggccgtc cgacccgcgt ccgaacgctt ggagtctgtg cgggcatggt gctggtcgcg
      361 ctcgccggcg cgatcgtcgt caggggcgac ggatttgatt ttcgcatgcc tgcggaaatt
      421 cgcgcgatgg ccagcgtacc gacgcaagcc gccaaatggc gggtccacga atgcctgctc
      481 gatctcaagc atgagacgac ctttgccgac gattgcgtcg acgccaaccg gcggccgctg
      541 gtgttcgtct ggggcgattc aacggccggc gcgttgatcc ccggcctgcg cgaggcgcag
      601 cagcggcgcc agttcggcat cgcgcagttc acgggctcgt cctgccctcc cgtgctcgat
      661 accgacttcg cctttcagcc gaattgccgg gcgatgaacg acaaggtctt tgcgctggcg
      721 agccagctca ggcccgagat cgtgctgttg cacggcatcc atgagatgtt cctcgatgcc
      781 gccgccgaga ccgtcaccag gctcaagcgg gaaacgggag cgcgcgtggt cgtgctcggt
      841 gccgagccgc aatggaatcg cgggctgccc aacgaagtgc tcaggcatta catgctctac
      901 catcgcctga tcccagcacg caccttcgat tcggtgctgc ccaactggca ggacgcgatg
      961 ttccgcgcaa agctcgtgcc gcgcggcgcc gagttcattt ccgcgcacga taccatgtgc
     1021 aacgcggagg gctgtcttgc gctgatcggc gatcgggcca gcgatatttc gacaagcgac
     1081 gtgatccatc tgaccgagaa gggttcgaca ttcctgatcg gggcgatcat cgaccaggtg
     1141 ctggatgggt cggcggagag gcgtgcgagc gcgttgcact gacgccagct ttcacgcgcg
     1201 gcgcgcgatg cggccgttct ccacgttgaa aatctcgctg gatgcaccga tctcggtaaa
     1261 ggccgcggga tcggcgccag tcagccagat ctgcgcgccg agcttttgaa gctctgcaaa
     1321 cagcgcggcg cgcctgttgg gatcgagatg cgcgaccacc tcgtcgagca ggagcagcgg
     1381 tgtgatcccc gtggtctccg cgaccagcgc cgcatgcgca agcacaagcc cgatcaggag
     1441 tgccttctgc tcgccggtcg aagcgtcgcg cgcgggcatg cctttcggcg cgtaaatcac
     1501 ctcgagatcg gtcagatgcg gcccgtcgag cgtgcgcccg gcggccgcgt cgcgggcgcg
     1561 gttgtcgcgc aggatctttc tgtaacgatc ttcgacggcc gttgcgggct ccgacaacag
     1621 cgcattttcc atccagccgt cgagtgcgat ctcggcggat ggaaaggccg aagcctcgcc
     1681 gcgcgcgcgc aatcgggagg cgagcttgac cgcggtctgg ccgcgttggg ccgcgaccgc
     1741 gaccgcaagc tccgcggtct cgcgctcgat cgcgtcgcac caatggtcgt cgaaattgcg
     1801 gacttccagg aggcggttgc gcgagcgcaa agagcgatcg agagccgaga cgcggctcga
     1861 atgttcgctg tcgatggcaa gcacgaggcg gtcgaagaag cgccgtcgtt cggaggcggc
     1921 gccgaggaac agtccgtcca tcgccggcgt cagccacacc atgcgcaaat gatcgccgaa
     1981 ggccgctgcc gagcccaccg tctcgcgatc gatacggcag cggcgcgttg cggacgcatc
     2041 gccctgcggt gcatcgatgc cggtgccgag cgtggccagc cccagcgcgc cctcgatctc
     2101 ggccgcaacc gcccaggagc cgtcgccctg attgtcggcg acatcttcca gcgtggcgcg
     2161 gcgaagcccg cgccccggcg acaggaacga gatcgcttcc aggcaattgg tcttgccggc
     2221 gccgttcggc ccgacgagcg ccaccatgtc gccccgcgtc tcgaaatgag ccgtgcggta
     2281 attgcggaaa tgcgtcagcg tcaggcgatg gatgcgggac ggggtcatga tcggtgtcta
     2341 ttgcgccgtc atgtccggct tgaggccggg cacccacgtc cttcgcgcgc aaagacgtgg
     2401 atggccggga caagcccggc catgacgcaa tccagggcag catgctccca caaagggaga
     2461 gggtttcatc acacccgcat cggcatcagc acatagagcg cagtcttgtt gtcgcggtcc
     2521 tggatcaagg tcggcgagcc cggatcggca agcttcaaca ccgcagcctc gccctcgatc
     2581 tgggcggcga tgtcgagcaa atagcgcgag ttgaaaccga tatcgagcgg atcggcggcg
     2641 tattcggctt cgatttcctc ggtagcgctg ccggaatccg gattggtcac ggaaagcacg
     2701 agcttgcccg cagtcaacgc cagcttgacc gcgcggccgc gttcgctcga aatcgtcgag
     2761 acacggtcga cggcggcggc gaaatcgttc ttgtcgacga ccagttcctt gtcgttgttc
     2821 tgcgggatga cgcggccgta gtcggggaag gtgccgtcga tcaatttcga agtcagcacg
     2881 acattgccga tggtgaagcg gatcttgccg gccgacaatt cgacggcgac ttccgcctca
     2941 ttgtcctcga tcagccgctg cacctcgccg accgtcttgc gcggcacgat cactccgggc
     3001 atgccgacgg cgcccttcgg caaggcgaga tcgacctgcg ccagcctgtg gccgtcggtt
     3061 gcgaccgcgc gcaggctcgc agccttggcg ctgccggccg aatgcagata gatgccgttg
     3121 agatagtagc gggtctcttc cgtcgaaatc gcaaactgcg tgcggtcgat cagtcgcttc
     3181 acgtcggcgg ccgtaagcgt gaaggagtgc gtcatctcgc cggcggcgag atccggaaaa
     3241 tcgctctcgg gcagcgtctg cagcgtgaag cgcgagcgcc cggcgcggat cgccagtacc
     3301 gcgcggtcgc catcgccctc gagcaggatt tgcgcgccgt cgggcagctt gcgcacgatg
     3361 tcgtagaaca tgtgcgccgg gacggtcgtc gctccgccag ttgcggtttc ggccgcgagc
     3421 gtctccgtca cctcgagatc gagatcggtt gccttcagcg atagctgcgc gttgtcggcg
     3481 cgcagcagca cgttgccgag gatcggaatg gtgttgcggc gctcgaccac gcggtggacg
     3541 tggcccagcg atttcaggag ttgcgcgcgt tcgacggtga ccttcatagc aatatccgcc
     3601 cgatccaaca ggacatgtgg tgggaatgcc gggcggtcaa agcgccgcgg cctcgtggca
     3661 cccggtaagc cacggaggtg ggctttcggg ggagcgtaaa gtggcgcgga tagggcgcaa
     3721 gttcaaggga ccggtggccc gttccccacg ttttgggctg tttggctcgc ctttatctac
     3781 ccctggaagg caggccggtt tgcgcatccg gcgcaaaccg gggagggaat ctctcgcttt
     3841 ggtcctgcaa gaccccccgt ccgacgcccc ccaatgcggg ggagcgagag caaaatgctc
     3901 cggggttaag aaagcctatt cctgcaattg ccttttgagc gattccacct cttccgacag
     3961 cgccacatcc ttggcgacaa gggcctcgat cttgcgcacc gcgtgcagga cggtggtgtg
     4021 atcgcgcccg ccgaagcggc gcccgatctc gggcaaggaa cggagcgtca gcgtcttcgc
     4081 caggtacatc gccacctgcc gtggccggac cacattggcg gtgcggcgcg acgacaacag
     4141 gtcggagcgg ctgacattat attgccgcgc caccacgcgc tggatgtcct cgatcttgat
     4201 ccgcttcggt tcctgcgggc ggatcaggtc gcgcacctcg cgctcggcca tgtccagcgt
     4261 taccggctgg ttgttgagct tggagtgcgc gagcaggcgg ttgatcgcgc cttcgaggtc
     4321 gcggccgtta tgggtgatgg tgcgggcgag ataatcgagc acctggtccg gaacgtcgaa
     4381 gcttgcatga tgggcacgcg ccgccgcgac gcgcgacttc aggatgccga gcctcagttc
     4441 ttcgccgagc gatgccatct cgaccacgag tccgcctgcg agccgcgagc gcacgcggtc
     4501 gtcgaggctt tctagatccg acggcggccg atccgccgcg atcacgacct gacggccggc
     4561 atcgatcaac gcgttcagcg tgtggcagaa ctcggcctgc gtggattttc cctgcagaaa
     4621 ttgcaggtcg tctatcacga gcacgtcgat gccgcgcagc gcttccttga aggcgagcgc
     4681 ggtttgcgtc ttcagcgcgg cgacaaagcc gtacatgaat ttttccgccg tgagatacag
     4741 caccttgcgc tcgctgccgg cattgcccgc ccaggtcacc gcctgcaaca aatgcgtctt
     4801 gccgagtccg acgccggcgt ggatgtaaag
//
LOCUS       DATLJT010001198         3695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1238209,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001198 DATLJT010000000
VERSION     DATLJT010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238209"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VM910_27540"
     CDS             complement(<1..963)
                     /locus_tag="VM910_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI66308.1"
                     /translation="MAHETETLAAYVAELKFEDIPPEVLERAKVLTLDFLGSAIRARR
                     DAESTPSLMQMLEALALDGKGESTVFGDDKTWTPAVAALLNGALGHSLDFDDTHADSS
                     LHASAPVVPAAFAVGEMMGSSGRNVLTAIVAGYEVCCRLGNALDPTSHYARGFHPTAT
                     AGTYGAAAAAAKLFGLSQAQIVSAFGVSGSQAAGSLQFLVNGAWNKRYQVGASAMNGV
                     IAATLARNNFVGSIESIEGRHGLLVGYSDNPHPDKAVAGLGSTYETMMIGVKPYPSCR
                     YTHAALDAIIAMRREHNLTPDQIKRVEIGLHRNGITLTGDAATKR"
     gene            complement(1105..1764)
                     /locus_tag="VM910_27545"
     CDS             complement(1105..1764)
                     /locus_tag="VM910_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI66309.1"
                     /translation="MTPSVDLFFSFRSPYSYLALPKTMKLVGDYDMTVNLRPVYPLAV
                     REPGFFKKTNPKFARYVVLDSSRVAQYEKIPFRFPVPDPIVQNMATLEVAEHQPYIHR
                     LTRLGALAQLEGRALVFTYAIARVLWDGTVTGWNKGDHLARAAEKAGFDLAAMDEAIA
                     ADPDRYEGVIAANEKDHAESGHWGVPTFVFEKEPFFGQDRIDLLIWRMQSKGLTCRDG
                     R"
     gene            complement(1780..3192)
                     /locus_tag="VM910_27550"
     CDS             complement(1780..3192)
                     /locus_tag="VM910_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVI66310.1"
                     /translation="MTSIDKADRVSDQDAARSKRGDLSREFAETVRLAAPMALTQLSQ
                     LAMMTTDLAFIGRLGGEAVAAAALAGTVYWVSFTFGMGLMSAVAPLAAQAFGAGEAAL
                     VRRSLRTGLWAALLIALPIMLFSLRGEQILLSLGQATVPAHLAQQYLFGLAWGVAPAL
                     WFIAIRGFMGAVNRPEPVLWIMLAAIPANALLVYPLLYGEWGLPRLGLFGAGLATTSV
                     NFATVLAGMWFAVHREPFTDYRVLTRFWHLDWSLMRQLVAIGAPISIALLLEFGLFSA
                     AALLMGRISTTAIAAHQIALQVAALLFMVPYGIGLAATVRVGHAVGRNDTAGVWRAGI
                     VAMLLGVALGAILTIIEIVSRFAIARFFLGEGLTNADATIELAATLLLVGATFFITDG
                     QQTIAAGALRGMKDTRVPLLFAAISYWLIGFTLCCWLGFFTPLGAVGVWIGLSIGTFV
                     YAVLLIFRFRLLSRRLAERF"
BASE COUNT          711 a         1180 c         1200 g          604 t
ORIGIN      
        1 ccgcttggtg gcggcatcgc cggtcagcgt gatgccgttg cggtgcagcc cgatctcgac
       61 ccgcttgatc tgatcgggcg tcaaattgtg ctcgcgccgc atcgcaatga tcgcatccag
      121 cgcggcgtgg gtgtagcggc agctcggata gggtttcacg ccgatcatca tggtctcgta
      181 agtcgagccg aggcccgcga ccgccttgtc gggatgcgga ttgtcgctgt agccgaccag
      241 gagcccgtgc ctgccttcga tcgattcgat cgagccgaca aaattgttgc gcgcgagcgt
      301 cgccgcaatc acgccgttca tcgcggaggc gccgacctgg tagcgtttgt tccaggcgcc
      361 gttgaccaga aactgcagcg agcccgccgc ctggctgccg gagacgccga aggccgacac
      421 gatctgggct tgcgagagcc cgaacagttt tgcggccgcc gccgcggcgc cgtaagtccc
      481 tgcggtcgcg gtcggatgga agccgcgcgc gtaatgcgag gtcggatcga gcgcattgcc
      541 gagccggcag cacacctcgt aaccggcgac gatcgcggtc agcacgttgc gcccggaact
      601 gcccatcatc tcgccgaccg cgaatgccgc cggcaccacc ggcgcgctcg catgcagcga
      661 cgaatccgca tgcgtatcgt cgaaatcgag ggaatggccg agcgcgccgt tgagcagcgc
      721 cgcgaccgcc ggcgtccagg tcttgtcgtc gccgaagacg gtcgactcgc ccttgccgtc
      781 gagcgcgagc gcttccagca tctgcatcag cgacggcgtc gactccgcat cgcgccttgc
      841 gcggatggcg ctgccgagga aatcgagcgt cagcactttg gcccgctcca gcacctccgg
      901 cggaatatct tcgaatttca gctcggcgac ataggctgcg agcgtttcgg tttcatgcgc
      961 catggcgtta cctctctttg ttccgtcatt gcgaagccag gcaatccatc gtgtcaggat
     1021 cggaagaccg gattgcctcg ccgctttcgc tccgcgcaag gacggtgact ggtcgtccgt
     1081 aggtacggcg ggggcaggga ggcgtcaacg tccatcacgg caagtcagcc ccttgctttg
     1141 catgcgccag atcaggaggt cgatgcggtc ctggccgaaa aacggctcct tttcgaacac
     1201 gaaggtcggc acgccccaat ggccggactc ggcgtgatcc ttctcgttgg ccgcgatcac
     1261 gccctcataa cggtcagggt cggcggcgat ggcctcgtcc atggcggcaa gatcgaagcc
     1321 cgccttttcc gccgcacgcg ccagatgatc tcccttgttc cagcccgtga ccgtgccgtc
     1381 ccacagcaca cgggcaatcg cataggtaaa gaccagcgcg cgtccctcga gctgcgccag
     1441 cgcgccaagc cgcgtcaggc ggtggatgta gggctggtgc tcggcgactt cgagcgttgc
     1501 catgttctgc acgatcggat cggggaccgg aaagcggaac gggattttct cgtattgagc
     1561 gacgcgactc gaatcgagca cgacatagcg ggcgaatttc ggattggtct tcttgaaaaa
     1621 gccgggctcg cgcacggcga gcggatagac cgggcgaaga ttgaccgtca tgtcatagtc
     1681 gccgacgagc ttcatcgtct tcggcagtgc cagataggaa taggggctgc ggaaggagaa
     1741 aaagaggtca accgatgggg tcatggaatt atccctggct caaaaccgct ccgccagccg
     1801 gcggctcagc agccggaagc ggaagatcaa aagcacagca tacacgaaag tgccgatcga
     1861 caatccgatc cagaccccga cggcgccaag cggcgtgaag aagccgagcc agcagcacag
     1921 cgtaaatccg atcagccaat agctgattgc ggcgaacaac agcggtaccc gcgtatcctt
     1981 catgccgcgc agcgcgccgg ctgcgatggt ctgttggccg tcggtgatga agaaggtggc
     2041 gccgacgagc agcagggttg cggcaagttc gatcgtggcg tcggcattgg tgaggccttc
     2101 gccgagaaag aaccgcgcga tcgcaaaccg cgatacgatc tcgatgatcg tcaggatggc
     2161 gccgagcgcg accccgagca gcatcgcgac gataccggcg cgccagacgc cggctgtatc
     2221 gttgcggccg accgcgtgcc cgacgcgcac ggttgcggca agcccgatgc cgtaggggac
     2281 catgaacagg agcgcggcga cctggagcgc gatctgatgc gcggcgatgg cggtcgtcga
     2341 gattcgtccc atcagcagcg ccgccgccga gaacagaccg aactcgagca gcagggcgat
     2401 ggagatcggc gcaccgatcg caacgagctg ccgcatcagg ctccagtcga ggtgccagaa
     2461 tcgcgtcagc acgcgatagt ccgtgaacgg ttcgcggtgc acggcaaacc acatcccggc
     2521 caggacagtg gcgaaattga ccgaggtggt ggcgagcccc gcgccgaaca ggccaagtcg
     2581 cggcaggccc cattcgccat agagcagggg atagactagc agcgcgttgg ccgggatcgc
     2641 ggcgagcatg atccacagca ccggctccgg ccggttgacc gcacccatga agccgcggat
     2701 cgcgatgaac cacagggccg gcgccacccc ccatgcgagc ccgaagaggt attgctgcgc
     2761 gagatgcgca ggcaccgtag cctgaccgag gctcaacagg atctgctcgc cgcgcagcga
     2821 gaacaacatg atcggcagcg cgatcagcag cgcggcccat aggcccgtgc gcaacgagcg
     2881 gcgcaccagc gccgcttctc cggcgccaaa ggcctgcgct gcgagcggcg ccaccgccga
     2941 catcaatccc atgccgaagg tgaagctcac ccaatagacc gtgccggcca gcgcggcggc
     3001 ggccacggct tcgccgccga gccgtccgat gaaggcgaga tcggtcgtca tcatcgcgag
     3061 ctgcgacagt tgcgtcagcg ccatcggcgc ggcaaggcgg acggtctcgg cgaactcgcg
     3121 cgacagatcg ccgcgcttcg agcgtgcggc gtcctgatcg gaaacccggt ctgccttgtc
     3181 gatggaggtc atgtgacgat catagcatcg tgtgtgacgg aacgatggag tttagaatga
     3241 acgcgcaaca gtcacgcgtt cccgcggcgc gatcgcgccc gggtgatgcc aagatcatgt
     3301 ccctcgaaaa tcaagagggt gcagggaaag ccggatgctg gctgcacccg cagcctcgcg
     3361 tgcagttgga agaagcacac gagtaagtcc actacaggtt caaccggagc ggcccggcct
     3421 tccctgcgcg aatggtttta acggtgtcct ccgtgatctc cccggtgtcc atgacttttt
     3481 agtcaccgtc gccggcaggt catcacctgt cgacttggca ccagccaagg ggtgccagga
     3541 ccacacgtct tcttccgtcc gcggccaaca tcattcgtcg aattgatgcg gcgcgcgtcc
     3601 atcgcatccc gtctcaacgt ccgtgacgac gcgtacgccc ctcatgtcga gtcgggacgg
     3661 cgggaagatg tgcatgattt ccgattttcg gaaag
//
LOCUS       DATLJT010001199         4101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2846923,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001199 DATLJT010000000
VERSION     DATLJT010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846923"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..244)
                     /locus_tag="VM910_27555"
                     /pseudo
     CDS             complement(1..244)
                     /locus_tag="VM910_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665310.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            complement(323..667)
                     /locus_tag="VM910_27560"
     CDS             complement(323..667)
                     /locus_tag="VM910_27560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66311.1"
                     /translation="MQAGAGGLLIPSSVYFGSQRRQLVVLAARHALPTVYNQREYAEA
                     GGLISYGPSQSDAYRRAGVYVGRILKGEKPGDLPVELGTKFDLVINLATAKAIGVEIP
                     PTLLALADEVIE"
     gene            826..1062
                     /locus_tag="VM910_27565"
     CDS             826..1062
                     /locus_tag="VM910_27565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66312.1"
                     /translation="MQHPEQLGQQRGNGKDDTRDIAARPIDACDQTNSGRVGSDDEYD
                     RRRRGCGFACLRRRNAGPYDNGDLPAHEIGRQSR"
     gene            complement(1011..1229)
                     /locus_tag="VM910_27570"
                     /pseudo
     CDS             complement(1011..1229)
                     /locus_tag="VM910_27570"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            1564..2874
                     /locus_tag="VM910_27575"
     CDS             1564..2874
                     /locus_tag="VM910_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66313.1"
                     /translation="MSSPQVTPKPLQIAAAVIGNALEWYDFIVFGFFTVIIARLFFPS
                     DSQYASLLLTTATFGVGFFMRPVGGILLGIYADRRGRKAALLMVIALMTVAIAMIACA
                     PTYAAIGVAAPFIIVIARLLQGFSAGGEFASATAFLTESAPAQRRGLYGSWQMVGQGL
                     AVLIGAVLGTLLTRWLTPQALDGWGWRIPFLFGLIIGPLGLYIRRNLDETSAFLQSRR
                     SSAGQQSSGSILASHVREMLACLGMVVSGTISFYVILIYIPTFARTQLHLPLDQAFLA
                     QSIGLACEIVLIPIFGWVSDLIGRKPVMIAALVLDLAVTYPLFSWVSASPSFGALLIM
                     QIILCSLFGVFNGPISTALAEQFPTRVRSTALAISYNIAVMLFGGFAQFFVTWLIEAT
                     GAPIAPAYYLMFGAAVGLLAALFLRERAHDAQLLVTEIVEPTAA"
     gene            3151..3297
                     /locus_tag="VM910_27580"
     CDS             3151..3297
                     /locus_tag="VM910_27580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66314.1"
                     /translation="MNIGRSLPRREDAKLLTGAGAFLDDLKPGAVTYARFVRSTVARL
                     TGSP"
     gene            3408..3788
                     /locus_tag="VM910_27585"
                     /pseudo
     CDS             3408..3788
                     /locus_tag="VM910_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3885..4101
                     /locus_tag="VM910_27590"
                     /pseudo
     CDS             3885..4101
                     /locus_tag="VM910_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612353.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          723 a         1245 c         1237 g          896 t
ORIGIN      
        1 atgagttcct atgtggccca tcaggatggt gtagccgtca ggtcttgcgc gcattgcgcg
       61 gagtgagccg gtcgtaccac cggctccaac cacattctca atgatgaact gctgtccaag
      121 cgtgcgcgcc atgtactcgc caacaatacg cgcactaacg tcggtgggac ccgccggcag
      181 caaacggaac gacaatcgta acgggcctca ctggataggc ttccgcgctt gccattcgcg
      241 acatagccgg tagcgcggaa gcgcctgcgg ccagatggag aaattgtcgg cggcgaagtt
      301 tcattttgaa cctcccttca catcattcga tcacctcgtc ggcgagtgcg agcagcgtgg
      361 gcggaatttc gacgccgatt gcctttgcag tggcaaggtt gatgaccaga tcgaatttag
      421 tcccgagttc gaccggcagg tcgccgggct tttcgccctt gagaatccgg ccgacgtaca
      481 caccggcgcg gcgataagca tccgactgac ttggtccgta gctgatcaga ccgccagcct
      541 cagcgtactc gcgttgatta tacaccgtcg gcagggcatg ccgggccgcg agcaccacga
      601 gttgccggcg ttgactgccg aaatatacac tgctggggat gagtagcccg cccgcgcccg
      661 cttgcacgac ctttgcgaag gcggcatgga attcacggcg ttcaccatca ggatttgccg
      721 tccgatggca cgggccgctt cctctaggtc tcgcgactga ccctcgacat caggaccgtt
      781 tgggtcgcgc aacacggcga tcattgacgc cttgggaacc aactcgtgca gcatcccgag
      841 caacttggcc agcaacgggg taacggaaaa gacgacaccc gtgatattgc cgcccggccg
      901 attgatgctt gcgaccagac caactctggc cgggtcggct ccgatgacga atacgatcgg
      961 cgtcgccgcg gttgcggctt tgcctgcctg cgccgccgga acgccggccc ctacgataac
     1021 ggcgacttgc ctgcgcatga gatcggccgc cagtcccggt agccgatcgt cttggccgtt
     1081 ttcaggttta tcactagctc gtatttggtc ggcgcctgca cggggaggtc ggcgggcttc
     1141 tcacctcgca gaatgcggtc aacgtaaccg gccgcacctc tatattggtc aatcaaatct
     1201 ggaccatacg agagcaatcc gcctgccttt acgaaagaac gatatgcata gactcacggc
     1261 agtcgatggg ctgcagcgtg ggctactatt gcctcgcgat acagcaccga tcgcgcgctt
     1321 acagggacaa atcagtgcca gctgctagac tagatgtccg ccaatatttg atgttttgtg
     1381 cgagtggcga catggccccc taccaagctc tacccgtctg cgatagcatt atcgaagaga
     1441 gctaccagta ggacgtgccg aagcgccgca attacctgcc gcaggctatc agctctttta
     1501 atttccactt gtttcaagag ccgtcaccgt caacgcttca tcccgagttg agtagaacag
     1561 ccgatgtcaa gcccccaggt cacgccgaag cctcttcaga tcgcagcggc ggtgatcggg
     1621 aacgctctcg aatggtatga cttcattgtc ttcggcttct tcacagtgat catcgctcga
     1681 ttgttctttc ccagcgacag ccaatacgcc tcgcttttgc tcaccaccgc gaccttcggc
     1741 gtcggctttt tcatgcgccc ggtgggcggg atcttgctcg gtatctacgc cgatcgtcgc
     1801 ggccgcaagg ccgcgctgtt gatggtcatc gccttgatga cggtagcaat cgcgatgatt
     1861 gcgtgcgcgc cgacgtatgc ggcgattggt gttgcggcgc cgttcatcat cgtgatcgcc
     1921 cggctgttgc agggcttttc ggccggcggc gagttcgcca gcgccactgc ctttctcacc
     1981 gagagcgcgc cggcgcagcg gcgcggcttg tatggctcct ggcaaatggt cggtcagggc
     2041 ctggccgtcc tgatcggcgc ggttttgggg acgctgctta cacgctggct gacgccccag
     2101 gcgctcgacg gctggggctg gcgtatcccc ttcctattcg gtctcatcat cggtccattg
     2161 ggactttata tccgccgcaa cctcgatgag acttcggcat tcctccaatc gcgccgcagc
     2221 tcggccggtc aacagagctc cggcagcatc ctcgcgtccc acgtcaggga aatgctggcg
     2281 tgcctgggaa tggttgtatc gggcacgatc tcgttctacg tgatcctcat ctatatcccg
     2341 acatttgccc gcacccagtt gcacctgccc ctcgatcagg cgttcctcgc gcagtcgatc
     2401 gggttggcat gcgagatcgt gctgataccg atattcggat gggtgtccga cctcatcgga
     2461 cgcaagccgg tcatgatcgc cgcgcttgtg ctcgatcttg cggtgaccta cccgctgttc
     2521 tcctgggtca gcgccagtcc atcgtttggc gcgcttttga tcatgcagat catactctgc
     2581 agcctgtttg gtgtcttcaa cggcccgata tcgacggcat tggccgagca gttcccgacc
     2641 cgcgtgcgtt cgactgcgct tgccatctct tacaacatcg cggtcatgct gtttggcggc
     2701 ttcgcgcagt tctttgtgac ctggttgatc gaggcaaccg gcgcgccgat cgcgccggcc
     2761 tattatctga tgttcggcgc ggcggttggg ttgctggccg ctctattcct gcgagagcgc
     2821 gcacacgatg cgcagctttt ggtcacggag attgtcgagc caactgccgc ctagtgattg
     2881 gcggatagga ctttgggaga agggccactt gataagtttg cattaattgt cttttgcgtc
     2941 cacgagccgg cggggccggc cggcgagccg tcgcacatct tccaccgtcg atgtgcttgc
     3001 cctgcgccac cccctccaat gggacggtgg tgacggtggc accccatggc gaagaaagat
     3061 ttaagctaat ttggctcgct ccttcgggga cgaacgcccc ttgctacccc ggcggtctag
     3121 tcgtccgcga gtgatgggac gaggcgcacg atgaacatcg gcaggtcatt gccgcgccgg
     3181 gaagacgcga agctcctcac cggcgccggt gcgttccttg acgatctcaa gccgggcgcg
     3241 gtgacgtacg cgcggttcgt ccgcagcacc gtggcgcgct tgaccggctc gccgtgaact
     3301 ggccacgctg aagcccgcat tctccggatg acttgttgat tttggcaacg ggcgccgcga
     3361 ttcagatata gcgatcagca gcttatctgg gattggggga ggcggcgatg gcgaacgcag
     3421 cgggtgaatc ggaatcgagt gcacttcagc tcgattttga tcgccgtctg atactgcagt
     3481 ttcgtggctc cgtgatcacg tccgatgccg gagtgctcgc ctatcgcgaa cttgacgacg
     3541 tgcttaggtt gaccgcattg gccggtgaga tgctcgctga tgcccgcacc ggcaagaacg
     3601 ggcgccatgc gctggtcggc ttgctgcggc agtcggtgtt cagccgtctc gcgggctacg
     3661 aggatgtgaa cgatgccgaa cgtcttcgtc acgatcctgc gatgcgctgt atcgtcggcg
     3721 gcaaggctgc tcacggccgc gcggcctcac caagccagat gggccacttc gagacaccgt
     3781 ggcttgcggc agagacgaac ctttctgtac tgactagggt cagaactcat taatttttcc
     3841 gctctgataa cgatcccgat tcgttttgga agaggattgg gatcgtgcgc aggaacctgt
     3901 tttggctcag cgatgagcaa tggaagcgga tcgaacccca cctgccgagg gatgtccggg
     3961 gcgtcgagcg ggtggacgat ccccgcgtca tcagcggcat tgtgcacgtg ctgaagagcg
     4021 gctgtcgctg atgcgattgc ccgcccgagt atggtcctcc gacaacgatc tataaccgct
     4081 ttgtgcgctg ggcgcggcgc g
//
LOCUS       DATLJT010001200         9717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_776263,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001200 DATLJT010000000
VERSION     DATLJT010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776263"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..373
                     /locus_tag="VM910_27595"
     tRNA            284..373
                     /locus_tag="VM910_27595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:318..320,aa:Ser,seq:cga)
     gene            528..1775
                     /locus_tag="VM910_27600"
     CDS             528..1775
                     /locus_tag="VM910_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI66315.1"
                     /translation="MAREMEKLTALRVERVTRAGMYGDGGGLYLRVTADGAKNWVFRF
                     MLNGRPRWMGMGPLHTISLAEARKRAGEHRLRRHDGIDPIEARRAKRLQAQLEAAKAI
                     TFKQCAESYIKAHRAGWKNAKHAAQWGATLATYAEPIIGNLSVQAIDTALVLKVLEPI
                     WTAKPETAGRVRGRIESILDWAKVREYRQGENPARWRGHLDKLLPARGKVRKVEHHAA
                     LSYAELPGFMIELREQESIAARALEFTVLTAARTGETIFARWGEINLLDKTWTIPSER
                     MKAHREHRVPLSARALATLAEMQAHCTGDDAFVFPGGKAQKPLSNMAFLMLLRRMGRG
                     DLTAHGFRATFKTWASERTSFQNEVVEAALAHVTGSKVEQAYQRGDMFEKRRRLMQQW
                     ATFCTTAPAQERPSNVAPMRRIG"
     gene            3100..3435
                     /locus_tag="VM910_27605"
     CDS             3100..3435
                     /locus_tag="VM910_27605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66316.1"
                     /translation="MSTTTPPVSNAGDSPSAGATLSEKLRALRDQGSAPQSTPAPAQT
                     PAARIETAVEQKARPEVAARQRLPSRRVAETFELEVGGLRYTATIGRFPDGRIGELFL
                     NNHKSAPPT"
     gene            3587..5245
                     /locus_tag="VM910_27610"
     CDS             3587..5245
                     /locus_tag="VM910_27610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI66317.1"
                     /translation="MIELRPYQTDVVDQVEQLLGTAARPLIVAPTGSGKTIIFCEIIK
                     RAVAKHKRVLVLAHRREIIKHTCNKLRAHGIRHGIIMAGEDKALRPQERVQVAAIATL
                     WVRAVRSGAIPLPPADLIVIDEAHHAPARTYQRLVEAYPNATVIGGTATPCRGDGRGL
                     GGIFTVLAEAPQVAPLIQQGHLVKSRVYAPIDPNLKGITVRNGDYAENELADRMDRAK
                     LVGDVVTHWHKYAERRRTVAFACSVGHSVHIRDEFVKSGVRAQHLDGTTPTDERDDIL
                     ARLASGETEVVTNCMVLTEGFDCPDIGCIVLARPTRKMGLFRQMIGRGLRPADGKSDC
                     IILDHSGAVFRHGLPEDHVEWSLDPDTRAEAPAHQARLEHKVPGLLECSQCGALRLGG
                     KPCPCCGFLPQRPAKYVRHVEGELGLVSGGQAQAPRYTLEQKAEWYRMLVGEVLRRGK
                     KPGWAFYLFQDKFGHGPLQHWDRTALIPTPEVASTSARGSSPTPRSEVRHERHRSATR
                     ASARPLARHPDSDRHRCRLSHPQKRAMSDLSGRPRPLAVLRYQR"
     gene            5298..6005
                     /locus_tag="VM910_27615"
     CDS             5298..6005
                     /locus_tag="VM910_27615"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVI66318.1"
                     /translation="MKFTGLPFKEAAQRIEMVIGAEPITIKAKPVADPRPKLRRMWRD
                     AKPTMPDDVVDTYLRSRRVGLDVYPSTIRTAPGLRCYEDDTTNTFPAMLAVVRDITGK
                     PVTIHRTYLAADGSGKAPVEKPRKIVSKHGTSPHIRLTPVASVTGIAEGIETAIAAMK
                     LFKVPTWSVLSTYGIETFEPPAGLQRLIVFADHDANGAGQKAAHLLAARLAGQIPIEI
                     RFPDTTGDDWNDVLRRR"
     gene            6002..6523
                     /locus_tag="VM910_27620"
     CDS             6002..6523
                     /locus_tag="VM910_27620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66319.1"
                     /translation="MRTVVYLRRAEIIVIARRKHVADTADLDRFLVAWFWHRPKSADA
                     DPIGTLIHVAWRMGRANLTSAEAEEIISASKRGRPLYKADDLGEYLRLTDRERTAWGI
                     RTIGGHDVSKRQRALRCKQKAKERQARFRQQRGATPHARSLSRTKPWETEGISRRTWE
                     RHRKQEQEQEAAE"
     gene            6520..6867
                     /locus_tag="VM910_27625"
     CDS             6520..6867
                     /locus_tag="VM910_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66320.1"
                     /translation="MKPLKIASMTTTVCKHQDRLIAESIAEAKAWGASLRLTEAARIA
                     TGAVTVASIDATSEQYDQWAQQYIRLLFFAEGTRCAKCGHVATGPLTHYSAEDAKRWQ
                     RADLAANTPVANS"
     gene            7360..7560
                     /locus_tag="VM910_27630"
     CDS             7360..7560
                     /locus_tag="VM910_27630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66321.1"
                     /translation="MNTQAILRAAAVEMLRSGLTTQAEVAALAHTSRQLVKYWAAREE
                     IDTVETRKAWLRKVWREAIRQI"
     gene            7912..8248
                     /locus_tag="VM910_27635"
                     /pseudo
     CDS             7912..8248
                     /locus_tag="VM910_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388434.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            8463..8666
                     /locus_tag="VM910_27640"
                     /pseudo
     CDS             8463..8666
                     /locus_tag="VM910_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(8705..9484)
                     /locus_tag="VM910_27645"
     CDS             complement(8705..9484)
                     /locus_tag="VM910_27645"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI66322.1"
                     /translation="MRKPVLVVAQQIQLRARIARALHSAGYGAELAGNQKRALELAAR
                     KDIQAAIVVHSTDLAGLRQKLLGKIPKTILLGHQTDEIVRPGHSLQGTDASLDEQKLL
                     GRLSQPGALPGSKRDETVPAPVKIEDCKLDLAGYTFVDGSGREIRLTRAETALLTALV
                     DSPRRVLSRDQLRYAVAGRGAEPYDRSVDMLVSRLRRKIEPDPKAPRFILSVPGIGYK
                     FDVRPQSAANGEAIDPEQFNRSRRGEAIGYSVRRGNLPRKD"
     gene            complement(9523..>9717)
                     /locus_tag="VM910_27650"
     CDS             complement(9523..>9717)
                     /locus_tag="VM910_27650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66323.1"
                     /translation="AGLTDDLIVRDQLLDLAQEWMLTARRARRPSDDTRVVSLHKNLG
                     DNVMPLHTNQDDDDGSPGLG"
BASE COUNT         1960 a         3068 c         2961 g         1728 t
ORIGIN      
        1 gcgcggccaa gttcctgctg ctgcccgagg ccggccacaa gggcaacacc cacatgctga
       61 tgatggacag gaacaacctt gagatcgccg acttgatcat cggctggctc ggcgagaccg
      121 cggccgcgcg acagtcggag agtcgtcgct aaggccttcc accatgtccc cgcgatcgaa
      181 ccccacgatg atccgcgcga ggcgacagag cgttcttgtt tagccgtctc gcgttggata
      241 catacctgcc gaccgacagg gtgtttgcgc cttttgcggc cacggagagg tggctgagtg
      301 gttgaaagca ccgcactcga aatgcggcat acgggtaacc gtatcggggg ttcgaatccc
      361 tccctctccg ccagaagcct cattcgcgat ccttcaccgc cattcaccac acaccacaaa
      421 agacccggta tttagccaac aagtgtgatg tttttgcctc tggacacgtt cgcagtcctt
      481 cgccatagtg cgcgcgatct ttgtggacag aattgtagat tttagaaatg gccagggaaa
      541 tggagaaact gacggcgctt agggtcgagc gcgtaacgcg agctggcatg tacggcgacg
      601 gtggcggcct ttacctccgc gtcaccgcgg acggcgcgaa gaattgggtt ttccgtttca
      661 tgctcaatgg ccggccgcgg tggatgggca tggggccatt gcacacgatc agcctggccg
      721 aagcacgcaa gcgggccggc gaacaccgcc ttcggcgcca cgacgggatt gatccgatcg
      781 aggcgcgccg cgccaagcgc ctccaagcac aactcgaagc agccaaggcc atcacgttca
      841 agcaatgtgc ggagagctac atcaaggcgc atcgcgcggg ctggaaaaac gcaaagcacg
      901 cagcgcaatg gggagcaacg cttgcgacct atgccgagcc gatcattggc aatctctcgg
      961 tacaggcgat cgacaccgcg ctcgtgctca aagtcctgga accgatctgg acggcgaagc
     1021 ccgagacggc gggccgcgtg cgcgggcgca tcgagtcgat cctcgactgg gcaaaagtgc
     1081 gcgagtatcg gcaaggcgag aacccggcac gctggcgcgg ccatctcgac aagctgttgc
     1141 ctgcgcgcgg caaggtgcgg aaggtcgaac accacgccgc attgtcctac gccgagttgc
     1201 cgggcttcat gattgagttg cgcgagcagg agagcatcgc ggcgcgcgcg ctcgaattta
     1261 cggtcctcac ggccgccaga acgggcgaga cgatcttcgc gcggtggggg gagatcaact
     1321 tactggacaa aacttggacc ataccgagtg agcgcatgaa agcgcaccgc gagcaccgcg
     1381 ttccgctttc cgctcgtgcg ctcgctaccc tcgcggaaat gcaggcccac tgcaccgggg
     1441 atgatgcctt cgtgtttccc ggtggcaagg cgcaaaagcc gctctccaac atggcgttct
     1501 tgatgctgct gcgccgaatg ggtcgcggcg acctgaccgc gcacggcttt cgcgcgacgt
     1561 tcaaaacctg ggcgagcgag cgcacctcgt ttcagaacga ggtcgtggaa gcggctttgg
     1621 cgcatgtcac cgggagcaag gtcgaacagg cctaccagcg cggcgacatg ttcgagaagc
     1681 ggcgccggtt gatgcagcaa tgggcgacgt tctgcaccac cgcgcccgcg caagagagac
     1741 cgagcaacgt cgcaccgatg cggcggatcg gctaacatcc aacagcggcg gatagggtag
     1801 ctcccgacac ggctggtacc gcaccggcct tccgccgcga ccttctgcgg agcgccagcg
     1861 gaagcgccca atggcataca aatctaaagc cgaacgcgcg gccgaacgcg cggccgcatc
     1921 tggaatgatg tggggcgaac tcatcaccca cgtccgagac gttgcgcgct gcgacgaacg
     1981 cgaagctcgc cggcaaatcg gaaacgccat cgaggatcga aacgagcgtc gccctttttg
     2041 gcgtcctccg ggcccaatgg acttccgcgc accggatgaa ccaccgcgcg atgccgctta
     2101 ctggcttgag tgcaaggccg atcccgacga tcccgaccgc atacaggaac ggcgttatga
     2161 tcccaatcga gtggacaagc gcacagcggc acgactcgat aggaaggcac gttttcgcaa
     2221 accaattttt tcacgcgagc aagtgctggc actccccggc ctcgaaccta gcggttccac
     2281 tgcggcctac gcgccagccc gcagatttct cgcggcgcta ctgaaaacca atcctcgcct
     2341 aactttcaag gaggcgttgc acgcgtgccg caagaaatgc ggacaactaa gtgagcgcgg
     2401 gtttcgtgct agcgtctggc cgcaggctcg agaggccgcg ggattgccgg caacagggag
     2461 ccctggccgt cggccggaac cctcgcgcta aacccttgcg gtaaaccctc gcgccatttt
     2521 tttcctgacg cctattacgc gctgtcatcg tgctgcatcg ctagtgtgcg cgctcgtgtt
     2581 tgggcgcgcg caaaacggag gctagcgatg agcacctgca acactgaacg cccaaaggcc
     2641 ggagcatcgg cgccgatcgc ggtcacgata gcggaggcct gccgtcagac tggcctcggc
     2701 caaacgacaa tctggaaatt tgctggcgag gggcgcctcg aacttatacg ccctccaggg
     2761 attaggcgca cactggtgag ctaccccagc ttggtgaaac ttctcacgcc atccaccgag
     2821 gcgccgcgcc gaaaacgcgg tcggccacgc aaggccgagc agcaagtgca gcataccgcg
     2881 ccgaccccgt caccacgtcg ccgtggtcgc ccgcggaaaa ctgctgcaca gctacagccg
     2941 gagaccgctg cagccaccac gtagaaaccc ccggacttcg cgcgtctagc ggaagtgccg
     3001 ggggccaatc gtctaagcaa ccaccaatcc aatccggtct tagcatttag gtcacgtctt
     3061 tcgcaagtgc gcggtcctca acccgaaagg gaccgctgca tgagtacgac cacaccgccg
     3121 gtttcgaatg ctggggacag ccctagcgcg ggcgccacgc taagcgaaaa gctgcgggcg
     3181 ctacgtgacc aggggtcagc gccgcaatcg actcctgccc ccgcgcaaac gccagccgcg
     3241 cgtattgaaa cggcggtgga gcaaaaggcg cggccggagg tggctgcgcg acaacggctg
     3301 ccgagccgcc gcgtggccga gacgtttgag ctcgaggtcg gcggactgcg ctacacggcg
     3361 acgatcggtc gatttcccga tggccgtatc ggcgagctgt ttcttaacaa tcacaaatcc
     3421 gcgccgccga cgtgaatgca ggggatgcgg cgatcgtctg ctcgatcgcc atccaatttg
     3481 gcgccgacat cgagataatc cgacaagctt tgtgtcgcga tagtcagggt cgggcttccg
     3541 gcccgctcgg cgctgcactc gatcgcattg ccgcggagag cgcgccgtga tcgagcttcg
     3601 cccctatcag accgatgttg tcgatcaggt cgaacaactc ctcggaaccg cagcgcggcc
     3661 attgatcgtc gcgccgaccg gcagcggcaa gacgatcatt ttttgcgaga tcatcaagcg
     3721 cgcggtcgcc aaacataagc gcgtgctggt gctcgcgcat cgtcgtgaga tcatcaagca
     3781 cacctgcaac aagctccgcg cgcacggcat tcgtcatggc atcatcatgg ccggcgaaga
     3841 caaggcacta cgcccgcagg agcgggtgca ggttgccgcc attgccacgc tctgggtccg
     3901 tgccgtccgc tcgggcgcca taccgctgcc acccgctgat ctgattgtga tcgacgaggc
     3961 acaccacgcc ccagcacgga cctatcaaag gcttgtcgag gcatatccga acgccacggt
     4021 gatcggcggt accgccacgc catgccgcgg cgatgggcgc ggcctcggcg gcatcttcac
     4081 tgtcctggcc gaagcgccac aggtcgcgcc gctgatccag caaggacacc tcgtcaagtc
     4141 gcgggtctat gcaccgatcg atcccaatct caaaggcatc acggtccgca atggcgacta
     4201 tgccgagaat gagcttgccg accgtatgga tcgcgccaag ctggtcgggg acgtcgttac
     4261 ccactggcac aagtacgccg agcgtcggcg cacggtcgcg tttgcctgca gcgttggtca
     4321 cagcgtccac atccgcgacg agtttgtgaa atctggcgtc cgtgcccaac atctcgacgg
     4381 caccacgccg accgacgaac gcgacgacat ccttgcccgc ctcgcatccg gtgagaccga
     4441 ggttgtcacc aactgcatgg tgctgaccga gggcttcgat tgccccgaca tcggctgcat
     4501 cgttctcgcg cggccgaccc ggaagatggg attattccgc cagatgatcg gccgtggctt
     4561 gcgtcctgcc gatggcaagt ccgactgcat catcctcgac cattccggcg ccgtcttccg
     4621 gcacggtctg cccgaagatc atgtcgagtg gtcgctcgat cccgataccc gtgctgaggc
     4681 ccccgcacat caagcgcggc tcgagcacaa ggttcccggt ctgctcgaat gcagccaatg
     4741 cggcgcgctg cggctcggcg gcaagccgtg cccatgctgc ggattcctgc cgcagcgacc
     4801 agcgaaatac gttcgccacg tcgaaggcga gctcgggctc gtgtctggcg gccaggcgca
     4861 agctccccgt tacacgctcg agcaaaaagc tgagtggtac cggatgctgg tcggggaagt
     4921 gctccgccgc ggcaagaaac ccggctgggc cttctatctg ttccaagaca agtttggcca
     4981 cggacccctg cagcattggg atcgaaccgc gctcatccct acaccggagg tggccagcac
     5041 gtccgctcgc ggatcatcgc ctacgccaag gtccgaggtg cggcatgaac gccatcgttc
     5101 cgctacgcga gcgagcgcgc ggccgttggc ccggcatcct gacagcgatc ggcatcgatg
     5161 ccggctatct cacccgcaaa aacgggccat gtccgatctg tcaggacggc cgagaccgtt
     5221 ggcggttctt cgataccaac ggtgacggta cctggaactg ccgctattgc ggcggcgggc
     5281 tcggcatcga tctcgtcatg aagttcaccg gcttgccgtt caaggaggcg gcgcagcgga
     5341 tcgaaatggt catcggcgcc gagccgatca ctatcaaggc caagccggtg gccgacccgc
     5401 ggccgaagct gcgccggatg tggcgcgacg ccaagccgac aatgccggac gatgtggttg
     5461 acacctacct tcgctcccgc cgtgtcggtc tcgacgttta tccgagcacc atccgtaccg
     5521 cgccaggcct gcgctgctac gaggacgaca cgaccaacac cttcccggcc atgctcgcgg
     5581 ttgtacgcga catcaccggc aagccggtca ccatccaccg cacgtacctc gccgccgatg
     5641 gcagcgggaa ggcaccagtc gagaagccgc gcaagatcgt cagcaagcac ggcacgagcc
     5701 cacacatccg cctgaccccg gtcgcgtcgg tgacggggat cgcggaaggg atcgagaccg
     5761 cgatcgcggc gatgaagctg ttcaaggtgc caacctggtc agtgctctcg acctacggca
     5821 tcgagacatt cgagccgccg gctggcctgc agcggctgat tgtttttgcc gaccacgacg
     5881 ccaacggcgc tggccagaaa gccgcacacc tgctggccgc acggctcgcg ggccagatcc
     5941 cgatcgagat caggtttcca gacacaacgg gcgacgattg gaacgacgta ctgaggcggc
     6001 gatgaggacc gtcgtctatc tgcgccgcgc cgagatcata gttatcgctc gacgcaagca
     6061 tgttgccgac accgccgacc ttgatcggtt cttggtcgcg tggttctggc atcggccaaa
     6121 atcggccgac gcagacccca tcggcaccct gatccatgtt gcctggcgga tgggcagggc
     6181 caatctgacg tccgcggaag ccgaggaaat catcagcgcg tccaaacgcg ggcgaccctt
     6241 gtacaaggcg gacgaccttg gcgaatacct gcgactgacc gatcgcgagc gcacggcgtg
     6301 ggggatcaga actatcggtg ggcatgatgt ttccaagcgc cagcgggcac tccggtgcaa
     6361 gcaaaaggcc aaggaacgac aagcccgctt tcgccagcaa cgcggcgcca caccacacgc
     6421 tcgctctcta tcgagaacaa agccgtggga aacggaaggt atcagccgac gaacatggga
     6481 acggcatcga aaacaagagc aagagcaaga ggcagcagaa tgaaaccttt gaaaattgcg
     6541 agcatgacca caacggtctg caaacaccaa gaccggctca tcgccgaaag catagccgag
     6601 gccaaagcat ggggcgcatc ccttaggctg accgaggcgg cgcgcatcgc aaccggcgcc
     6661 gtcaccgtcg cctcgatcga cgccacctcc gaacagtacg atcaatgggc acagcaatac
     6721 atccggctgc tgttctttgc cgagggtacc cgatgcgcca aatgtggcca cgttgccaca
     6781 ggcccgctca cccactattc ggccgaggac gccaagcggt ggcaacgtgc cgacctcgcc
     6841 gccaacacac ctgtcgcaaa ttcgtaacgc tccgttgacg caaattcgtg cgcaataacc
     6901 tttctttcct tgaccacgaa cttgcgacaa ccgagagaaa gagctttggc ggtagggttt
     6961 atgaactcga tctctgatct gacgacggta cacttggaga gatggcctcg cggccggcga
     7021 ccacgatgcg gagacgattt tgtctcagcg cgtgcgtttg ccggtttttt cgggagcgcg
     7081 ccgtcgccgg ttccagcccc aagcgacgcg ctcgccgaca aaagaccggg gattgagcga
     7141 gtgatgaagg taaggctcaa accgcgccgt gattgttgtc ggattggctg agttgctagc
     7201 gaacccgcca tcgggcggca ttcggcgcat gtgtcgggtt gtaggataac gggtttaacg
     7261 cttgtcacaa ttgacctgtt cctcggtagc cgcggccctg ccggcctatc atcgtgatcg
     7321 gcccgatgcc cgcgagagcg aagcacgagg gaccccgcaa tgaataccca agctatcctg
     7381 cgcgcggccg cggtagaaat gctgcgatcg ggccttacca cgcaagccga ggtcgccgca
     7441 cttgcgcaca cgtcgcggca actggtgaag tattgggccg cgcgcgagga gatcgacacg
     7501 gtggaaaccc gcaaggcctg gctgcgcaag gtgtggcggg aagccatccg gcaaatctga
     7561 tcaggcccgc ttaatatacg tgtgatttca atttgctgcg ccagtttctt cgagcgacgt
     7621 ccaaaacttt cgccgcgggc gggtaggccc gacagcacga acaacaggct tttcgcgcgg
     7681 cccccacgca gcagcgaacg gatcgggcgc gcgcacgcct caattatggc gcgaggttcc
     7741 gttttcgatc atggccgttg tccgcacact cggagcactt cgctaacccc aaagtgaagg
     7801 tcagatttag atgccgtaat gggccctgca ccgagcgagc ggtgaaggtg gctgtatgaa
     7861 acggggtcac gacgttgagt cggccccagg catctgagat ggaggacagc cgtggaatac
     7921 tatgcaggaa tcgacgtctc tttggaactc tcgagcgttt gcgttgtgga tgggcggggc
     7981 aagatcgtga aggaggcgaa ggttgcgagc gagccggaag ctcttgttta ttttttcaag
     8041 gaacttggct ttccggtgaa tcggattagg tttgaggcgg ggccgttatc acaatggctg
     8101 catgccgggc tgacgcaagc gggatttgaa gccgttctgc tggagacgcg gcacgtgaag
     8161 gcggcattgt cggcgagcat cagcatggct gattccacga cactccggga ctgcacacct
     8221 aggccgtgtt gacagtttta atccaatgct gattaacgtc tcggcgcgca cgctcgtggc
     8281 tgcgcgcccg cgcgcgatga cacggcaacc ttgcacacat ggggtggccg acgcatggcg
     8341 actatgctgc ggcagggtgc gacgagtagc cccgccgcca actctgctga tgcttctgtc
     8401 gtcgctgtgg gcatgtcgct ctcacgaagc ttttcagcgg aggcggccga gatcccgata
     8461 aaatcccctc tcaggcggta cccgaactcg acggccagga aaacatctgg cagttcatgc
     8521 gtcagaatta tttgtcaaac cggattttca aatccttcga cgatatcgtc gaccactgct
     8581 gcgcttggaa cacactcgtc gaccaaccct ggaagattat gtccatcgcg cgacgcgact
     8641 gggcagcagt cggtcactca atctgaggat tggtataaca gccgcaggct ggccccttca
     8701 accttcaatc cttccttgga agattgcccc ggcgcactga atagccgatg gcctctccgc
     8761 gtctggaccg gttgaactgt tccgggtcaa tggcttcgcc attcgcggcg ctctgcggcc
     8821 gtacgtcgaa tttatacccg atgcctggca ccgagaggat gaatcgtggc gccttcgggt
     8881 ccggctctat cttgcgtcgc aggcgggaga cgagcatatc gacgctacga tcatagggtt
     8941 ccgcgccacg cccggcaaca gcgtaacgga gttgatcacg cgacagtacc cggcgaggac
     9001 tgtctaccaa cgcggtcagc agcgcggtct cggcgcgggt caaccgtatt tcccgaccgc
     9061 tgccatcgac aaaggtatag ccggccaggt cgagcttgca gtcctcgatt tttaccggcg
     9121 ctgggacagt ttcatcgcgc ttgctccctg gcaacgcccc tggttggctg agccgaccga
     9181 gcagcttctg ttcgtccagt gatgcatctg tcccctgaag cgaatggccc ggtcggacga
     9241 tctcgtcggt ttggtgcccc agcaagatcg tttttggaat ctttccgagt aacttctgcc
     9301 ttaggccggc caggtcagta ctatgcacga cgattgccgc ttggatgtct ttacgcgcag
     9361 cgagctcaag cgcccgcttc tggttcccgg cgagttcggc gccataacca gcggaatgca
     9421 gcgcgcgcgc aattctagct cggagctgaa tttgttgcgc tacaactaga acgggttttc
     9481 tcactgcacg gtcctcggga aacttgcatt cgtgtctaca aatcagccca ggcccggact
     9541 cccgtcgtcg tcgtcttggt tcgtgtgcaa tggcatcacg ttgtcgccta ggttcttatg
     9601 caatgacacc acgcgcgtat catcgctggg gcgacgcgct cgccgtgcag tgagcatcca
     9661 ctcttgagcc aaatcgagaa gctgatcccg gacaatcagg tcatctgtca gtccggc
//
LOCUS       DATLJT010001201         5900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2386375,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001201 DATLJT010000000
VERSION     DATLJT010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386375"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VM910_27655"
     CDS             complement(<1..770)
                     /locus_tag="VM910_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HVI66324.1"
                     /translation="MGKRLIPPEPSEVHEIPLREALEERYLQYALSTIMHRALPDSRD
                     GLKPVHRRILYGMRLLRLNPGGLPKKSAKIVGEVMGSFHPHGDQAIYDAMVRLAQDFN
                     SRYPLVDGQGNFGNVDGDNPAAMRYTEARLTEVAQLLLDGIDEDAVDFRPTYDASGQE
                     PVVLPGGFPNLLANGAQGIAVGMATAIPPHNAAELCDAALHLIEKPNAKSRALLKWVK
                     GPDFPTGGIVVDSKEAIAEAYATGRGSFRTRAKWMQEEG"
     gene            complement(1747..2016)
                     /locus_tag="VM910_27660"
     CDS             complement(1747..2016)
                     /locus_tag="VM910_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66325.1"
                     /translation="MLLRAIGAAALTLSFSLSVTSNALAGSQPGLMRRVSCTVVRYYV
                     ERYSASAAETWARSHGATDAQIDAARRCLKDTPSQTAQAAGWDTR"
     gene            complement(2164..2919)
                     /gene="recO"
                     /locus_tag="VM910_27665"
     CDS             complement(2164..2919)
                     /gene="recO"
                     /locus_tag="VM910_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVI66326.1"
                     /translation="MEWTDDGIVIGVRRHGESAAIVELLTRAHGRHLGLVRGGAGSRM
                     RPLLQPGNGVRTVWRARLDEHLGIYAIEGTKLRAASLLASSHAVYGVTHLAALARLLP
                     ERDPHEDIYDLLEHTLDDFEDVGRAAVHLVRFELAMLAELGFGLDLENCAATGETADL
                     VYVSPKSGGAVSRAAGEPWRDRLLRLPPFLRPGEGGAAGWSNQDLLDGFQLTGLFLLR
                     HVLEPRGQGHSDARDGFINAVVKRRAHLLTSSS"
     gene            complement(3025..3405)
                     /locus_tag="VM910_27670"
     CDS             complement(3025..3405)
                     /locus_tag="VM910_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66327.1"
                     /translation="MKVPRNVLWFEALLYVSLMLDALSVAFQDRTPTAELTEQMILAA
                     TMVAGAMILLLVYFVWLAARQRKNWPRWVLAAALLLSVISLFQVIGENGLELDSSIEI
                     VSCVLTAAGLYFSFTGDARGWFNA"
     gene            complement(3424..4347)
                     /gene="era"
                     /locus_tag="VM910_27675"
     CDS             complement(3424..4347)
                     /gene="era"
                     /locus_tag="VM910_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVI66328.1"
                     /translation="MVSEPQIHTTATRCGFVALIGAPNVGKSTLVNALVGSKVTIVSR
                     KVQTTRALIRGIAIEGNAQIILVDTPGIFAPKRRLDRAMVSTAWSGAHDADLVCVLLD
                     AKLGLDEEADRILTRVAGVDHEKILVLNKIDLVPREKLLSLAKAVNERLTVAQTFMIS
                     ALSGDGVDDLRQALVKMVPPGPFLYPEDQMSDAPMRQLAAEITREKIFQQLHQELPYQ
                     STVETDSWTERKDGSIRIEQTIFVEREGQRKIVLGKGGATIKSIGAKSRKELAEITGQ
                     PVHLFLFVKVRENWGDDPDRYREMGLEFPKE"
     gene            complement(4449..5267)
                     /gene="rnc"
                     /locus_tag="VM910_27680"
     CDS             complement(4449..5267)
                     /gene="rnc"
                     /locus_tag="VM910_27680"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVI66329.1"
                     /translation="MSDDTPDIKTAPIASEAGHEPEPARVRKPARKKRSKAAKAKAAT
                     ASIEGRIGHKFTDSGLLTTAITHVSALKPTRKRGDSYQRLEFLGDHVLGLIVSDMLYR
                     SFPDADEGELSKRLADLVRKESCAEVAKALGLADDIKLGSVGAGAGARLRKSVLGDIC
                     EAVIGAVYLDGGYPAASAFVERNWTERMKKPRQPLRDPKTVLQEWAQGKGLPTPVYRE
                     VERTGPHHDPQFRVAVDLPGLAPAEGVGGSKRAAEKVAASVMIEREGVAGGNND"
     gene            complement(5264..>5900)
                     /gene="lepB"
                     /locus_tag="VM910_27685"
     CDS             complement(5264..>5900)
                     /gene="lepB"
                     /locus_tag="VM910_27685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVI66330.1"
                     /translation="SGSMKATLLVGDYLFVSKYSYGYSHYSIPLSPPLFSGRIFGSEP
                     SRGDIVVFRLPKDDSTDYIKRVIGLPGDRIQMKDGLLNINDKPVQRERLSDFIGEDPC
                     GSDATARVKRWKETLPNGVSYETLDCVDNGFYDNTNIYVVPPGHFFMMGDNRDNSTDS
                     RVLSAVGYVPFENIVGRAQMIFFSIAEGEHVWAIWRWPTAVRWNRLFKIVR"
BASE COUNT         1114 a         1864 c         1780 g         1142 t
ORIGIN      
        1 ccctcctcct gcatccattt ggcgcgggtg cggaacgaac cgcggccggt ggcataggct
       61 tcggcgatcg cttccttgga atcgacgaca atgccgccgg tcgggaagtc cggccccttc
      121 acccatttca ggagggcacg cgacttcgcg ttcggctttt cgatcagatg cagcgctgca
      181 tcgcagagtt cggcagcatt atgcggtggt atcgcggtgg ccatgcccac cgcgatgccc
      241 tgcgcgccgt tggcgagcag gttcgggaag ccgccgggga ggacgaccgg ttcttgaccg
      301 ctcgcatcat aggtcggacg aaaatcgacc gcgtcctcgt cgatgccgtc cagcaacaac
      361 tgcgcgacct cggtcagtcg cgcctcggtg taacgcatcg cggcgggatt atcaccgtcg
      421 acattgccga aattgccttg cccgtcgacc agcgggtagc gcgagttgaa atcctgcgcc
      481 agccgtacca tggcgtcata aatcgcctgg tcgccatgcg gatggaacga ccccatcact
      541 tcgccgacga ttttggccga cttcttgggc aatccgcctg gattgagcct gagcagccgc
      601 atgccgtaga ggatgcgacg gtgcaccggc ttcaagccgt cgcgcgagtc cggtaacgcc
      661 cggtgcatga tggtcgagag cgcgtactgg agatagcgct cttccagcgc ctcgcgcagc
      721 ggaatctcat gaacctcgga aggttccggc gggatcagtc ttttccccat aaatcctatg
      781 ctttcaatga tttctcatcg cgtttcgggg tttctcccag tcacgatgag aagcgactca
      841 accaaatata tagcagccgg ccgatgcgat tgcttccctt cccggtccag ggcgaggtca
      901 gcccgtcatc catcaagatg gctcagcatg tgagatgctt tagcatggcc tcacaggctt
      961 gttcggcagc ggtgaaggtt ttgaacggcg agccgatgac cttcacagcg gacctatttt
     1021 gatgaatcgg acgccaggac gctacgaagc cgggattgcc ctgaaggccg ggactacccc
     1081 tgctctcaaa actgatcacg aaggaaaagc cgctgctact cgcattccat atctggatgt
     1141 cctcaatggg agtgataacg cggctgaact gcacggcaca cctcacatgc gcctgcccct
     1201 aaaaaacagg gacacacatt tgccgagtcg aaaaatgcag ccttgttgac ctaatgcacg
     1261 cttttcccgc cgggtgggct ttctcggtgt ctctttctcg atgtctcgat gtctaaagac
     1321 gccgctgccg ggcgtcgacc cgcccgagga cgcgcgaggc cccgccggac cgggtagcca
     1381 cagagcgccg cgttaactct tccttaacca tgtgcatcca gcctcgggtt taggtgggca
     1441 agtgcttcgg aaacgtcagc gatcttgtcc cgatgactac tttttcaatt gttcatcatt
     1501 gggcgacggg cgacgcccga ggggctccat ggagcaacgg catgtcaggg cgtgaggtgc
     1561 aagccgatgt gaatgtacta tcggcgttcg cgttgatgat tggtggcgcg ctgggtatcg
     1621 gccttccgat cacgttgatt attatttggg tttgttcctg atcgttccga ggtgcgggtc
     1681 gatgggcgta ccccgattcg gtcgccggaa accaaccggc tctgactgcg gtaacgtgtc
     1741 cagcggctac cgcgtgtccc atccggccgc ctgagcggtc tgagacggtg tgtccttgag
     1801 gcatcgccgc gcggcgtcaa tttgtgcgtc ggtcgcccca tggctgcgcg cccacgtttc
     1861 cgcagccgag gcggaatacc tctccacgta atagcggacg acggtgcatg agacgcggcg
     1921 catcaagccc ggctgactgc cggcaagtgc gttggaggtc acagataacg aaaaggatag
     1981 cgtcagcgcc gcggcgccga ttgcacgcaa caacatcgta tttcctgtaa gtgccccaag
     2041 tgccacccca ccttgcagtg aatcacactc atattgcgaa cgcaaattca aactgcacgc
     2101 gcgaaacgac tgtcaccagt tattcgcgta cggaacagac gtggttgcgc tgttcgcgcg
     2161 ccgtcacgat gaagatgtga gcagatgtgc ccttcgcttc accaccgcgt tgatgaagcc
     2221 gtcgcgggca tctgaatggc cctgcccgcg cggttcaagc acatggcgca gcaggaacag
     2281 gccggtgagc tgaaagccgt ccagcagatc ctgattcgac cagccggcgg ccccgccctc
     2341 gcccggccgc agaaatggcg gcagcctgag cagccggtcg cgccagggct cgccggccgc
     2401 gcgcgacacc gcgccgcccg atttcggcga gacataaacc agatcagcgg tctccccggt
     2461 cgccgcgcag ttctccagat cgagcccgaa gccgagttcg gccagcatcg caagctcgaa
     2521 tcggacgaga tgcacggcag ctctgccgac atcctcgaaa tcgtccagtg tatgctccag
     2581 caaatcgtag atgtcttcgt gcggatcgcg ctcgggcaac agccgtgcca gcgcagcaag
     2641 gtgcgtcacg ccatagacag catgggagga cgcaagcagg ctcgctgcgc gcagcttcgt
     2701 cccttcgatc gcgtaaatgc caagatgctc gtcgagccgc gcccgccaga ccgtgcgcac
     2761 gccgttgcca ggctgcaaca aggggcgcat ccgcgacccc gcgccgcctc gcacgaggcc
     2821 aaggtggcgg ccatgcgctc gggtcaggag ttcgacgatg gcggcagatt cgccatgccg
     2881 ccgcacgccg atcacgatgc cgtcgtcggt ccattccatg gggcaaagcg tatcatggag
     2941 ggttggctaa gcgaagcgag cccaccgaga cgatcaacat ccgctgccac attgtcgtgg
     3001 taggcacgaa acgcgccttc cgcctcaagc gttgaaccag ccccgcgcat caccggtgaa
     3061 ggagaaatag agtccggccg cggtcagaac gcaggacacg atctcaatgc tgctgtcgag
     3121 ttccaggccg ttctcgccga tcacctggaa caacgagatc accgacagca agagggctgc
     3181 ggcaagtacc cagcgcggcc agttcttgcg ctgccgcgcc gcaagccaca cgaaatagac
     3241 cagcagcagg atcatggcgc cggccaccat cgttgccgcc agaatcatct gctcggtcaa
     3301 ttccgccgtc ggtgtgcgat cctggaaggc gaccgagagg gcgtccagca tcagcgagac
     3361 atagagcagc gcctcgaacc acagcacgtt tctggggacc ttcatcgcaa tcgccgtttc
     3421 ttgttattcc ttgggaaact cgagccccat ctcgcggtag cggtcgggat cgtcacccca
     3481 gttttcgcgc actttgacga acaagaacag atgcacgggc tggcccgtga tctccgcgag
     3541 ttccttgcgc gatttcgcgc cgattgactt gatggtggca ccgcccttgc cgagcacgat
     3601 cttgcgctgc ccttcgcgct cgacaaagat cgtctgctcg atccggatcg agccgtcctt
     3661 gcgttcggtc cagctatcgg tctcgacggt cgactgatac ggcaattcct ggtgcaattg
     3721 ctgaaagatc ttctcccggg tgatttcggc cgcgagctga cgcatcggcg catccgacat
     3781 ctgatcctcg ggatagagga acggccccgg cggcaccatt ttaaccagcg cctgcctgag
     3841 atcatcgaca ccgtcgcctg acagcgccga gatcatgaac gtctgtgcga ccgtcaaccg
     3901 ttcgttgacg gctttggcga gcgaaagaag tttttcccgc ggcacgagat cgatcttgtt
     3961 cagaaccagg atcttctcgt ggtcgacgcc ggcaaccctg gtcaggatgc gatccgcctc
     4021 ctcgtcgagt ccgagtttag catcgagcag cacgcagacg aggtcggcgt catgcgcccc
     4081 gctccaggcg gtcgacacca tggcgcgatc gagccgccgc ttcggcgcga agatgccggg
     4141 cgtatcgacc aaaatgatct gcgcattgcc ctcgatcgca atcccgcgga tcagcgcacg
     4201 cgtggtctgc accttgcgcg agacgatcgt gaccttggag ccgaccaacg cgttgacgag
     4261 ggtggatttg ccgacattgg gcgcgccgat cagcgcgaca aagccgcagc gcgtggccgt
     4321 ggtgtggatt tgaggttcgc taaccatcta accgcgctct cgccacgcaa acgtcaactc
     4381 caacctttac cgaaagccaa tattttccgg cggttctccc aacctgcgcg ctatgcgcgc
     4441 tgcccctgtc agtcattgtt gccgccggcc acgccttcac gttcgatcat cacggaagcc
     4501 gcgacctttt cggcggcgcg cttgctgccg ccgacgcctt cggcgggcgc cagccccggc
     4561 aggtccacgg cgacgcggaa ttgcgggtcg tggtggggac ccgtgcgctc gacttctcga
     4621 tagacagggg tcggcaggcc tttgccctgc gcccattcct gcagcaccgt cttgggatcg
     4681 cgcagcggct gccgcggctt cttcatccgc tcggtccagt tgcgttcgac gaaggcggaa
     4741 gcggccggat agccgccgtc gagatagact gcgccgatca cagcttcgca gatgtcgccg
     4801 agaacggatt tgcgcagacg ggcccccgca ccggcgccga ccgagccgag cttgatgtcg
     4861 tccgccagtc ccagcgcctt ggcgacctcc gcgcagctct ctttgcgcac gagatcggcg
     4921 agccgtttcg acaattcgcc ctcgtcggcg tcagggaaac tgcgatagag catgtcggac
     4981 acgatcagtc caagcacgtg atcgccgagg aattccaggc gctgatagct atcgcctcgc
     5041 tttcgcgtcg gcttcagcgc ggagacgtgg gttatcgccg tggtcagcag gccggaatcc
     5101 gtgaatttgt gcccgatgcg cccctcgatc gaagcggtcg ccgcctttgc cttggcggcc
     5161 ttgctccgtt ttttcctcgc cggcttgcgc acgcgagctg gttcaggctc gtggccagct
     5221 tcgcttgcga tcggtgcggt cttgatgtca ggggtatcgt cgctcatcgc acgattttga
     5281 agagacgatt ccagcggacc gccgtgggcc agcgccagat agcccagaca tgttcgccct
     5341 cggcaatgga gaagaagatc atctgcgccc ggccgacgat gttctcgaac gggacgtagc
     5401 cgaccgccga caacacacgg ctgtcggtag agttatcgcg gttgtcgccc atcatgaaga
     5461 agtgcccggg cggcacgaca tagatgttgg tgttgtcata gaagccattg tcgacgcaat
     5521 cgagcgtctc atagctgacg ccgttcggca gcgtctcctt ccagcgcttg acgcgcgcgg
     5581 tcgcatccga gccgcatggg tcttcgccga tgaaatcgct caagcgttcg cgttgcaccg
     5641 gcttgtcgtt gatattgagc agcccgtcct tcatctggat acggtcgccg ggcaacccga
     5701 tcacgcgctt gatgtagtcg gtcgagtcat ccttgggcag gcggaagacg acgatgtcac
     5761 cgcgcgaggg ctcggagccg aaaatgcgtc ccgagaacag cggaggcgac aacggaatgg
     5821 aatagtggct gtagccgtaa gaatatttcg aaacgaacag gtagtcgccg accagaagcg
     5881 tcgccttcat cgagcccgag
//
LOCUS       DATLJT010001202         1525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_319768,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001202 DATLJT010000000
VERSION     DATLJT010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319768"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VM910_27690"
     CDS             <1..220
                     /locus_tag="VM910_27690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66331.1"
                     /translation="EGLAPIIIQGLWQAIARLKEEGHSILLVEQNASLALQLVDYVHV
                     MSKGRVVYSARPDELRANDEIKSKYLGI"
     gene            420..>1525
                     /locus_tag="VM910_27695"
     CDS             420..>1525
                     /locus_tag="VM910_27695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66332.1"
                     /translation="MTVRRRTVLKGMLASSALVAAPATLRAQSGLMKIGFLTVKTGPL
                     ASGGIQMEQGLTLYLKERNNLLAGRPVQLFTGDSAGAPAMARTKMQEFLERENIACLI
                     GPLATAEALAIDDYIRDKQIPTLSVAAAEDMTQRKANPWFVRATSSSAQCSYPMGDYA
                     ARELKYKRAAMIADDIAYGYELNAGFQRAFEDAGGKVVQKLWPPLVSPDYGTYIAQLK
                     QDVDVLFIGFAGSNGFKFFKQYKEYGGKTPILGGMTAIDESLLQQMGDDAIGIISTCW
                     YSAQLDNPENKKFVEGMNRDYKVDPGFYAAATYTNGAVLEAALKKVDGRIEDKNALEA
                     ALRASDKIPTCRGPVSFDKYGNVVGNIYIRKVEK"
BASE COUNT          334 a          489 c          461 g          241 t
ORIGIN      
        1 cgaggggctc gcgcccatca tcatccaggg gctgtggcaa gcgatcgcgc ggttgaagga
       61 ggaaggccat tcgattctgc tggtcgaaca aaacgcctcg ctcgcgctcc aactggtcga
      121 ttacgtgcac gtgatgagca agggccgggt ggtctattcc gcgcgtccgg acgagctgcg
      181 ggccaacgac gagatcaaat ccaagtacct gggaatctga tggcgtcatc ggcgaagcga
      241 ctgctgatcc cgcataagac atcgcaatcc ttacaagaaa ttaatcgcct gcgagccgca
      301 gccggggtgt gacccgcggg acaagattgg tcccgatatg taaggatgat atccagcttg
      361 atgagaaaca agcgatggga gaaacccctc gccataaatc gaagccgaag ggaggagcaa
      421 tgaccgtccg tcgccgcacc gtcttgaagg gcatgctcgc atcgagcgcg ctcgttgccg
      481 cgcccgcgac cctgcgcgct caaagcggac tgatgaagat cggcttcctc accgtgaaaa
      541 ccggcccgct cgcctccggc ggcatccaga tggagcaggg cctcacgctc tatctcaagg
      601 agcgtaacaa cctgctcgcc ggccggccgg tgcagctctt caccggcgat tccgccggcg
      661 cgccggccat ggcgcgcacc aagatgcagg aattcctcga gcgcgagaac atcgcctgcc
      721 tgatcgggcc gctggcgacc gccgaggcgc tagccatcga cgactacatc cgcgacaagc
      781 agatcccgac gctctcggtc gccgccgccg aggacatgac gcagcgcaag gccaatccct
      841 ggttcgtgcg cgcgacctcg tcctcggcgc agtgcagcta tcccatgggc gactacgcgg
      901 cccgagaact caaatacaag cgcgccgcca tgatcgccga cgacatcgcc tacggctacg
      961 agctcaacgc cggcttccag cgcgccttcg aggacgccgg cggcaaggtg gtgcagaagc
     1021 tctggccgcc gctggtctcg cccgattacg gcacctatat cgcgcagctc aagcaggacg
     1081 tcgacgtcct tttcatcggc ttcgccggct ccaacggctt caagttcttc aagcagtaca
     1141 aggagtacgg cggcaagacg ccgatcctcg gcggcatgac cgcgatcgac gaatcattgt
     1201 tgcagcagat gggcgacgat gccatcggga tcatctcgac ctgctggtac tccgcgcaac
     1261 tcgacaatcc cgagaacaag aaattcgtcg aaggcatgaa ccgcgactac aaggtcgatc
     1321 ccggctttta cgccgccgcg acctacacca acggcgccgt gctggaagcg gcgctcaaga
     1381 aggtcgacgg caggatcgag gacaaaaatg cgttggaggc ggcgctgcgg gcaagcgaca
     1441 aaataccgac ttgccgcggc ccggtgagct tcgacaagta cggcaacgtg gtcggcaaca
     1501 tctacatccg caaggtcgag aagaa
//
LOCUS       DATLJT010001203         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4224222,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001203 DATLJT010000000
VERSION     DATLJT010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4224222"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..156
                     /locus_tag="VM910_27700"
     CDS             64..156
                     /locus_tag="VM910_27700"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66333.1"
                     /translation="MPIVFTIVGDPVGSDLVASLARPSGNWLLI"
     gene            224..1213
                     /locus_tag="VM910_27705"
     CDS             224..1213
                     /locus_tag="VM910_27705"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66334.1"
                     /translation="MRRREFITLLGSAATWPLAARAQHAGERMRRIGVLMNIPSNDEE
                     GQARITAFAQALLKLDWTEGGNVRTHTRWAADDVDRYRRYAEELVALTPDVILASGSP
                     SVEALLRVTRSVPIVFANVIDPVGAGFVTSMARPRGNTTGFTAYEYSISGKWLELLKE
                     FAPNVTRIAVLREPSIAAGIGQFAAIQSAASSSGVELSAIDSRDTGEIGRALAAFAHE
                     PNGGVIITASSSAVTHRELIISLAMRYRLPNVYPFRYYPASGGLASYGPDTIAEFKRA
                     SVYVDRILKGEKPADLPVQAPTKYETVINLKTAKALGLSVPEMLLARADEVIE"
     gene            1210..2196
                     /locus_tag="VM910_27710"
     CDS             1210..2196
                     /locus_tag="VM910_27710"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66335.1"
                     /translation="MKRREFITLGGAAAAWPLAARAQQREQMRRIGVLMPYPKDDPEM
                     GIRVRAFRQELAKLGWADGVNVQFDERWTADHMDRVWAEAASLIAANPDVILATGGRV
                     IPVLLQLSRSIPIVMPGASDPLRVGWATSLARPGGNITGFTMFEVSIFGKMLALLKQI
                     APAITRVAFIYNPDNPNTIVYRRTIEDTAGPIGIEPIAVPIHGFADIDRAVAALADRQ
                     NSGVLFPTDLTTFALRDEIVALIARHHLPAIYSDPAFTRAGGLAFYGPDRVDVFRRSA
                     GYVDRILHGEKAGDLPFQQPTKYVLILNLKTAKTLGLELSPTLLALADEVIE"
     gene            2193..2483
                     /locus_tag="VM910_27715"
     CDS             2193..2483
                     /locus_tag="VM910_27715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66336.1"
                     /translation="MRRRQFISLFGGMAAAWSLAALAQESATMGMTKATTAVLPQIEL
                     ARVKPVDRPPPNLRLSEGLIDLMKDFSLSSAQGIGGAWLRPSTPFDERYGQW"
BASE COUNT          542 a          807 c          847 g          522 t
ORIGIN      
        1 gtcgacgtca tcgtcacttg gggaactaca accgctctcg ctgcaaagca ggcgacatca
       61 acgatcccaa tcgtcttcac gattgtcggt gatccggtag gcagtgacct cgtagcaagc
      121 ctggcgcggc ccagcggcaa ctggttatta atctgacaac cgctaaggcg ctcgggctga
      181 ctgtgccgga taagctgctc gcactcgccg acgaggtgat cgaatgagac gccgcgagtt
      241 catcacgctg ctcggcagcg cggctacatg gccgctcgcg gcgcgcgcgc agcacgcggg
      301 agagcgcatg cggcgcatcg gcgtgctcat gaatattccc tcaaacgatg aggaaggtca
      361 ggcgcgcata acggcgtttg cgcaggcatt gctaaaacta gattggacag agggcggcaa
      421 tgtgcgcact cacacccgct gggcagctga cgatgtcgat cgctatcgcc gatacgcaga
      481 agaattggtc gcgctgacgc cggacgtcat ccttgcctcc ggcagtccga gcgtggaggc
      541 gctgctgcgg gtcactcgca gcgtgccgat tgtgtttgcg aatgtcatcg acccagttgg
      601 tgcgggattc gtcactagca tggcacggcc ccgaggcaat accaccggat ttaccgcata
      661 cgaatacagc atcagcggaa aatggctcga actgcttaaa gaatttgcgc cgaacgtgac
      721 gcgaatagcc gtccttcgcg agccctcaat agccgctggg atcggccagt ttgcagcgat
      781 ccagtccgct gcgtcatcat ccggggtgga attgagtgca atcgattcgc gcgatactgg
      841 cgagatcggg cgtgcccttg ctgcattcgc gcacgagccg aatggtggcg tcatcataac
      901 ggcaagctcg tcggcggtga cccatcgcga actgatcatt tcgctggcga tgcggtaccg
      961 cttaccgaat gtctacccat tccgatacta ccccgcgagt ggcggcctcg cctcttacgg
     1021 tcctgatacg atcgctgaat tcaaacgcgc ctctgtttac gtcgatcgca tcctcaaagg
     1081 ggagaagccc gccgatctcc cagtgcaggc accgacgaaa tacgagacgg tgatcaacct
     1141 aaagaccgcg aaagcgctgg gcctcagcgt gccggagatg ctgctcgccc gcgccgacga
     1201 ggtgatcgaa tgaagcgccg cgagttcatc acgctgggcg gtgcggcggc ggcgtggccg
     1261 ctcgcggcgc gcgcgcagca gcgcgagcag atgcggcgca tcggggttct gatgccttat
     1321 cccaaggacg atcccgaaat gggcatccgc gtccgggcgt tccggcaaga gctcgcaaaa
     1381 ctcggatggg cggatggcgt aaacgtgcaa tttgacgagc gatggactgc cgaccacatg
     1441 gaccgcgtgt gggctgaggc ggcaagtctg atcgcggcaa atccggacgt catcctcgcg
     1501 accgggggac gggtcattcc cgtattgctg cagctctcac gttcgatccc cattgtcatg
     1561 ccgggagcga gcgatcccct tcgtgttggc tgggcgacaa gcctggcgcg gccaggcggc
     1621 aacatcaccg gcttcacgat gttcgaggtc tcgatcttcg gcaagatgct tgcactgttg
     1681 aagcagattg cgccggcgat cacgcgcgtt gccttcatct acaatccgga caatccgaat
     1741 accattgtct acaggcgcac catcgaggac actgccggtc cgattggcat tgaacccatc
     1801 gctgtcccca ttcacggatt tgccgacatc gaccgtgccg tggcggcatt ggctgatcgg
     1861 cagaacagcg gagttttgtt tccgaccgac ctcacgacct ttgctttgcg cgatgagatc
     1921 gtagctctga tcgcgcgcca tcatttgccg gccatctatt cggatcctgc cttcacgagg
     1981 gcgggtggct tggcgtttta tgggcccgat cgcgtcgatg tctttcgccg atcggccggt
     2041 tacgtcgacc gcattctgca cggagagaag gccggcgatc tgccgttcca gcagccgacc
     2101 aaatacgtgc tcattctcaa cctaaaaacc gccaagacgc tcggcctcga attgtcgccc
     2161 acgctgctgg cgctcgccga cgaggtgatc gagtgaggcg gcggcagttc atcagcctgt
     2221 tcggaggcat ggctgcggca tggtcgcttg cagcgctggc gcaggagtcg gcgacgatgg
     2281 ggatgaccaa agctacgaca gcggtcttgc cgcaaatcga attggcgcgc gtaaaaccgg
     2341 tcgacaggcc gcctccgaat cttcgattga gcgagggcct gattgacctg atgaaggact
     2401 ttagcctttc gagcgcccag ggtatcggtg gtgcctggct gcgcccgtcg acgcccttcg
     2461 acgagcgcta cgggcagtgg tgaaaacact ttgtccccgg cggctgtaga aaaacatggc
     2521 ctcgacctcc atgcgccgac gaggtgatcg agtgatgtga aggaagaggg aggttcagaa
     2581 tgaaacttcc cttgcgccag gccaagtcca gaaaaggagg atggatgacc tgaaaccttt
     2641 tgcagcgatc cagagggtcg acgccctccc ggcaaaggcg agccagccag ccggaagtga
     2701 gtcttgcagg gatggtgg
//
LOCUS       DATLJT010001204         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1698477,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001204 DATLJT010000000
VERSION     DATLJT010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698477"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VM910_27720"
     CDS             <1..928
                     /locus_tag="VM910_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HVI66337.1"
                     /translation="RDLAILARAIIRDLPEYEYFVHIPSIRLGHRITQNFNRLIGRYP
                     GADGFKTGFICASGYNLVASATRNGKRLIAVVLGASSGTMRAVRAAQLLDRGFASNNG
                     LGWLRPSLGAVENLPPIDTSPPNLRDEVCGGKRHKPASDEDDALIAGVGGSSPSASTG
                     ETGASSTLFLASGLQPPMPKPSEWLAASPAPSEPIVVYTGPTRTGAALLAAVAADADR
                     QATPRRGKKSHVVAKKPDADEPKADNGSKADAKPAKPAAVRHASAKPDASAKSAAAPA
                     DTAKPAKPKATAKPKADSKPAQKGSSSDKPAS"
     gene            1111..2793
                     /locus_tag="VM910_27725"
     CDS             1111..2793
                     /locus_tag="VM910_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVI66338.1"
                     /translation="MERVWLKQYPAGVPADIDVTQYASLVALLEESFAKFANRKAFIC
                     MDKSISYRDLDEMSLALAAYLQSKGLAKGARVALMMPNVLQYPIATAAVLRAGCAVVN
                     VNPLYTPRELEHQLKDSGAEAIVVLENFAHTVQQVIGHTSVKHVIVGSMGEMLGLKGV
                     IVNLVVRRVKKMVPPFSLPGAVSFNDALAAGRAVKFNKPKIGPEDVAFLQYTGGTTGV
                     SKGATLLHRNIVANVLQNDAWLQPALAMPPKIDQIFIVCALPLYHIFALTACYLLAVR
                     AGGVNLLIPNPRDIAGFIKELMKYQVNSFPAVNTLYNGLMHHPDFNKIDFSKLKISNG
                     GGMAVQRPVAEQWKKITGCSIAEGYGLSETSPTLTCNTATSAEFNGSIGIPVPSTDIS
                     IRDDDGKELTFGQAGEICAKGPQVMTGYWNRPDETAKVMTADGYFRTGDIGVMSPDGY
                     IKIVDRKKDMILVSGFNVYPNEIEEVIASHPGVLECAVIGVPDERSGEAVKAFIVKRD
                     PNLTAEDVIKFCHEQLTNYKVPKHVEFRTELPKTNVGKILRRQLRDEKKAAA"
     gene            2820..3374
                     /locus_tag="VM910_27730"
     CDS             2820..3374
                     /locus_tag="VM910_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HVI66339.1"
                     /translation="MTEACNVTAADVLAFWRQAGADRWYSRDDAFDAEISNRYLDLWR
                     NAAAGELSTWEESDGGALALTIVLDQFPRNMFRGDPKTYASDAMARDVANRAIARGAD
                     LRVADDLREFFYLPFMHSEHLPDQLRCVDLFRKEGGADNLRYAEDHAAIIRRFGRFPH
                     RNHILRRATTAEEQAFLDQGGFSG"
BASE COUNT          676 a         1139 c         1045 g          568 t
ORIGIN      
        1 gcgcgatctt gccatccttg cgcgcgcgat catccgcgac ctgcccgaat acgaatattt
       61 cgtgcacatc ccctcgatcc gtttgggcca caggatcacc cagaacttca accggctgat
      121 cggccgctat cccggcgccg acggtttcaa gaccggcttc atctgcgcgt caggttacaa
      181 cctcgtcgcg tcggcaacgc gcaacggcaa gcggctgatc gcggtggtgc tgggcgcctc
      241 ctccggcacc atgcgcgcag tgcgcgccgc gcaactgctc gatcgcggct ttgcctccaa
      301 caacggattg gggtggctca gaccctcgct cggcgcggtg gagaatttgc cgccgatcga
      361 cacctcgccg ccgaacttgc gcgatgaggt atgcggcggc aagcgccaca agccggcgag
      421 cgatgaggac gacgcgctta ttgccggtgt cggcggcagt tcgccgtccg ccagcacggg
      481 cgagaccggc gcgagcagca cgctgttcct ggcctccggc ctgcagccgc cgatgccgaa
      541 accgtccgaa tggctggcgg cttcacccgc gccgtccgaa ccgatcgtcg tctacaccgg
      601 gccgacccgt accggcgccg ccctgctcgc agccgttgcc gcggatgcgg accgccaggc
      661 tactccgcgg cgcggaaaga agtcgcatgt cgtcgcgaaa aagcccgacg ccgatgagcc
      721 gaaggccgat aacggcagca aggccgatgc aaagcctgca aagccggcag ccgtcaggca
      781 cgccagcgcg aagcccgacg catcggcaaa atccgccgcg gctccggccg ataccgcgaa
      841 acccgcaaag cccaaggcaa ccgcgaagcc caaggccgac agcaagcctg cgcagaaggg
      901 ttcgtcgagc gacaagccgg ctagttgacc tgacgccggg tcgcaggcga gatccctgtc
      961 aggcgccgcc atcgcacgat ccagccgcgg caaagcgtcc ctcacatccg ccccgccggt
     1021 aacccccgac cgcttgccac gggcgctggc atggcccaat ctgcccaaaa caaccccgcc
     1081 tgagagacgg ggatgacgag agggaatacc atggagcgag tctggctcaa gcaatatccc
     1141 gccggcgtgc cggccgacat cgacgtgacc caatacgcct cgctggtcgc actcttggag
     1201 gagagcttcg ccaagtttgc caaccgcaaa gccttcatct gcatggacaa gtccatcagc
     1261 tatcgcgatc tcgacgagat gtcgctggcg cttgccgcct atttgcagag caaggggctc
     1321 gcgaaaggcg cacgcgtcgc gctgatgatg ccgaacgtgc tgcaatatcc gatcgcgacc
     1381 gcggccgtgc tccgcgccgg ctgcgccgtc gtcaacgtca acccgctcta tacgccgcgc
     1441 gagctcgagc atcagctcaa ggattccggc gctgaggcaa tcgtcgttct ggagaatttt
     1501 gcgcacaccg tgcagcaggt gatcggccat acctcggtca agcacgttat cgtcggcagc
     1561 atgggtgaga tgctcggctt gaagggcgtc atcgtcaacc tggtggtgcg gcgcgtgaag
     1621 aagatggtgc cgcccttttc gctgcccggc gcggtctcct tcaacgacgc gctcgccgca
     1681 gggcgcgcgg tcaaattcaa caagccgaag atcggacccg aagacgtcgc cttcctgcaa
     1741 tacaccggcg gcaccaccgg cgtctccaag ggagccacgc tgcttcaccg caatatcgtc
     1801 gccaacgtgc tgcaaaatga cgcctggctg cagccggcgc tcgcgatgcc gccgaagatc
     1861 gaccagatct tcatcgtatg cgcgttgccg ctttatcaca tctttgcgct gaccgcgtgc
     1921 tacctgctcg cggtgcgcgc cggcggcgtc aatctcctga ttccgaaccc gcgcgacatc
     1981 gccggcttca tcaaggaact gatgaaatat caggtcaaca gcttccccgc ggtgaacacg
     2041 ctctataacg ggctgatgca tcaccccgat tttaacaaga tcgatttctc gaagctgaaa
     2101 atctcgaacg gcggcggcat ggccgtgcag cgcccggttg ccgagcagtg gaagaagatc
     2161 acgggctgca gcatcgcaga aggctacggc ctgtccgaga cttcgccgac gctcacctgc
     2221 aataccgcga cctccgccga gttcaacggc tcgatcggca ttcccgtgcc ctcgaccgac
     2281 atctcgatcc gcgacgacga cggcaaggaa ttgacgttcg gccaggccgg cgaaatctgc
     2341 gccaaaggtc cgcaggtgat gacgggctac tggaacaggc ctgacgagac cgccaaggtg
     2401 atgacggcgg acggttattt ccgcaccggc gatatcggcg tgatgtcgcc agacggctac
     2461 atcaagatcg tcgatcgcaa gaaggatatg atcctggttt cgggcttcaa cgtctacccg
     2521 aacgaaatcg aggaagtgat cgcaagtcac cccggcgtcc tggaatgcgc cgtcatcggc
     2581 gtgccggacg aaaggtccgg cgaggcggtc aaggccttca tcgtgaagag ggatcccaac
     2641 ctcaccgcgg aggacgtcat caagttctgt cacgagcagc tcacgaacta caaggtgccc
     2701 aagcacgtcg agttcaggac cgaactgccg aagaccaatg tcgggaagat cctgcgccgc
     2761 cagctccgcg acgagaagaa ggccgcggct tgacggcagg gacggatgga ggcgcggccg
     2821 tgaccgaggc ttgcaacgtc acggcggctg acgttctggc gttctggcgc caggcgggcg
     2881 ccgatcgttg gtacagccgc gacgatgcct tcgacgcgga gatcagcaac cgttatctcg
     2941 acctctggcg caatgcggcg gccggagagc tttcgacatg ggaggagagc gatggtggcg
     3001 cgctggcgct taccatcgtg ctcgaccagt ttccccgcaa catgttccgc ggcgacccaa
     3061 agacctatgc cagcgatgcg atggcgcgcg acgtcgccaa ccgcgccatc gcccgaggcg
     3121 ccgatctgcg cgtcgcggac gatctgcgcg agttcttcta tctgcccttc atgcattcgg
     3181 agcatctgcc ggatcagcta cgctgcgtcg atctgttccg caaggagggc ggagccgaca
     3241 accttcgata cgccgaagac catgctgcga tcatcaggcg gttcggtcgt ttccctcacc
     3301 gcaaccatat tctgcggcgc gcgacgacgg ctgaagagca agccttcctg gaccaaggcg
     3361 gattttccgg ctgatgacgc aacgtttatc cgcgctcgcc agggttttgg aagacctcat
     3421 attgtacc
//
LOCUS       DATLJT010001205         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2618322,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001205 DATLJT010000000
VERSION     DATLJT010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618322"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VM910_27735"
     CDS             complement(<1..1587)
                     /locus_tag="VM910_27735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HVI66340.1"
                     /translation="MAQLTWRAVLIGSCLGGVLSLTNLYIGLKAGWGFGVAITASVLS
                     YAIWTSLLKIGLARTPMTILENNCMQSTASSAGYSTGGTLVSAFAAYIIINNQSMSVP
                     LMLAWVFFIAVLGVTMAIPMKRQMINIEQLRFPSGIAAAETLRALHAKSSQGLRAAKA
                     LGLAGLMAAIDKFWAEGLSTISASLDRFSSGALLTGLQKWLLGSHYEAWSGRTVIFSW
                     DFIFLAAGAITGMRVCATMFVSGTVCWAVVVPVLQTKGVIEGTGFAAIVQWTLWFGAS
                     CMVAAGVLSFALSWRTALSAFRDLGQMLSLRSAAASEGDRIETPMSWFAVGQIVSFVA
                     LAWLGHASFGMPVWETAIAVALSFWLALVACRVTGETDTTPVGAMGKVTQLIFGALSP
                     GNINVNLMSANITAGAATSAADLLTDLKSGYLLGANPRQQFLAQFSGIFVGTVVTVLT
                     FAVLVPNAQVLGTDQFPAPAAQTWSAVAIALGQGLSSLDSIKLWLIFAGGVVGVSLTL
                     APVLFPKYQEHLPSASAFGLA"
     gene            complement(1925..>2455)
                     /locus_tag="VM910_27740"
     CDS             complement(1925..>2455)
                     /locus_tag="VM910_27740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66341.1"
                     /translation="EVQRDYTMIEAPFPTHKAMLKEHKGDLVVGVLPFSYDPELNEFA
                     EPLFTTQSSLGRIALSFWVARKTFIDKNAAALLDLLEDYGTMLRWFYDPANHEEAVEI
                     VARFLKRPPAVLEDWLFTKKDFYRDLDGKPDLKVIQESIEKVKELGFIKETVDASKYA
                     DLSLIEEATRRRGSGN"
BASE COUNT          518 a          741 c          757 g          439 t
ORIGIN      
        1 ggcgagaccg aacgccgacg ctgagggaag atgctcctga tatttcggga ataaaacagg
       61 cgcgagcgtg agcgacactc cgacaacacc gccggcaaaa atcagccaca gcttgatcga
      121 gtcaagcgag gacagaccct gcccgagcgc gatggcaaca gccgaccagg tctgagcggc
      181 gggcgccggg aactggtccg tgccgagtac ctgcgcgttg ggcaccagga ctgcaaatgt
      241 gagcacggta acgaccgtac cgacgaagat gccggagaac tgggccagaa actgctgacg
      301 gggattcgcg ccaagcaggt agccgctctt taagtcggtg agcaggtcgg ccgctgaagt
      361 cgccgcaccg gccgtgatgt tggcgctcat caagttgaca ttgatgttgc ccgggcttag
      421 cgcgccgaag atgagctggg tcaccttacc cattgcgccg acaggcgtcg tatcggtctc
      481 gcccgtcacg cgacaggcga cgagcgctag ccagaaggac agcgctaccg cgatagcagt
      541 ttcccaaacc ggcatgccga agctcgcgtg cccgagccag gcaagagcaa caaacgacac
      601 gatctggccg acggcaaacc agctcattgg cgtctcgatg cggtctcctt cgctcgcagc
      661 cgctgagcgc aacgagagca tttgtcccag atcacggaat gcgcttaatg ccgtgcgcca
      721 gctcagcgca aaactcaaca ctccggcggc caccatgcaa gacgcgccga accagagcgt
      781 ccactgcacg atggctgcga aacccgtacc ttcgatgacg cctttcgtct gcagcactgg
      841 caccaccaca gcccaacata ctgtgcccga gacgaacatg gtcgcgcaga cccgcatgcc
      901 ggtgatggcg ccggcggcga gaaatatgaa atcccaggaa aagattacgg tgcggcccga
      961 ccaggcttcg tagtggctgc cgaggagcca cttctgcagg ccggtcagaa gggccccgct
     1021 ggaaaagcgg tcgaggctcg cgctgatcgt cgacagcccc tccgcccaga acttgtcgat
     1081 cgcggccatg agcccggcaa gacccagtgc cttggccgcg cgcagcccct gcgagctttt
     1141 ggcgtgcagc gcgcgtaagg tttcggccgc cgcgatgccg gaagggaaac gcagctgctc
     1201 gatattgatc atctgccgct tcatggggat ggccatcgtg accccgagca cggcgatgaa
     1261 gaacacccag gccagcatga gcggcacaga catcgactgg ttattgatga tgatgtaggc
     1321 cgcaaaggcc gagaccaggg tgccgccggt ggagtaacca gcggaactgg cagtcgactg
     1381 catgcagttg ttctcaagga tggtcatcgg cgtgcgcgcg aggccgattt tcagcaagct
     1441 cgtccagatt gcataagaca agacgctggc cgtaatggcg acgccgaagc cccagccggc
     1501 cttgagaccg atgtagagat ttgtcagcga caacacgcct cccaggcatg agccaatcag
     1561 caccgcgcgc caggtcagtt gcgccatgct gtcgccgcgg ccgcgataaa cctgctcgta
     1621 ccactgtcgc tcgatcgctt cgggtgcgcc tttgaagccg acgacaggta gcggccggtc
     1681 tcgccgatca gtagcttcga cagagagatc catcgatggc gcgaatcgtg ctggagggat
     1741 ttggggcacg gatgatggca accttcggcg aggcaagtcc gagctagccg gggctaccga
     1801 gagcgcgtcc tgactgctgg cgcgtatgat cgaagatggc gcgcttgtac cacaccttcg
     1861 gcgacgatcg agggcagggt cgatgtgtag cgcgtcgagt gtatcaactg tgagcgcaag
     1921 gtgttcagtt cccgctaccg cggcgcctgg tcgcctcctc aatcagactt aggtcggcat
     1981 atttcgatgc gtcgaccgtt tccttgatga atcctagttc cttgaccttt tcgatacttt
     2041 cttgaatgac cttaagatca ggcttgccgt cgagatcgcg gtagaagtcc tttttggtga
     2101 acagccagtc ttccaacacc gccggaggtc gcttcaggaa gcgcgcgaca atctcgaccg
     2161 cctcctcgtg gttggccgga tcgtagaacc aacgcagcat tgtgccgtaa tcttccagca
     2221 gatcgagcag tgcagcggcg ttcttgtcga tgaacgtttt ccgcgctacc cagaaagaca
     2281 gcgcgatgcg tccgaggctc gactgcgtcg tgaaaagggg ctcagcgaat tcgtttagct
     2341 cgggatcgta ggagaacggc agtacgccca ccaccagatc gcctttgtgc tccttgagca
     2401 tcgccttgtg ggtcgggaac ggcgcttcga tcatggtgta gtcgcgctgc acctc
//
LOCUS       DATLJT010001206         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3996213,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001206 DATLJT010000000
VERSION     DATLJT010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996213"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VM910_27745"
     CDS             <1..399
                     /locus_tag="VM910_27745"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVI66342.1"
                     /translation="WAGMKIDKVAGIEARGFILGGAVAHQVSAGFVPIRKKGKLPHKR
                     VSIAYSLEYGLDEMEMHEDAAEKGDRVILVDDLIATGGTAEGAVKLLRQLGAQVLAAC
                     FIIDLPDLGGAARLRKLDVPVRTLISFEGH"
     gene            508..1470
                     /locus_tag="VM910_27750"
     CDS             508..1470
                     /locus_tag="VM910_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66343.1"
                     /translation="MAVGAAALPALPRITRAQSYPSRLVTIIVPFAAGGTTDVIARIL
                     GEHMSRTLGQQFIVENVVGAGGTTGSTRAMRANPDGYTIELGNMGTHAASVALYPSLA
                     YNPEVDFAPIGMVAGLPVLVVGKKDLPANDLKEFATYVRANAEKLNMAHAGVGSITHF
                     TCLLLNSILGVKPTLVPFNGAAPAISALIGGQVDYMCNVAPDVVQQIQGGAFKAYAIG
                     GSKRNSALPDVPTSEEAGLPQFQASAWNALFAPKGTPKPILDKLSDALDKAIDDENTR
                     QRLLGLGSEIPDKARRGQQPLAALVKDEIARWTPIIKAAAVKSE"
     gene            1509..2456
                     /locus_tag="VM910_27755"
     CDS             1509..2456
                     /locus_tag="VM910_27755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66344.1"
                     /translation="MKLPRRKVLQLAAGAAALVAVAPMARAQAYPTRPVRIVVGFAAG
                     GSTDIGARLIGQWLQERLGQPFVIENRPGAGTNIATEMVVRAPPDGYTLLMVGPSSAV
                     NATLYDKLDFVFLRDIAPVASLIRQPQIMLANPSVMSKTVPELIADAKANPGKITMAS
                     AGVGSAGHLVGELFKMMAGVDFVHVPYRGAGPALADLLGGQVMISFAGIAGSIAYIRT
                     GKLRALAVTTATRSEALPDVPTVSEFVPGYEAGDWLGVGAPKHTPAAIVDKLNGEIAT
                     ALAIPRSRRALPISVAPRLRSRQPSSASSSPTKRRNGPK"
     gene            complement(2524..2787)
                     /locus_tag="VM910_27760"
     CDS             complement(2524..2787)
                     /locus_tag="VM910_27760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66345.1"
                     /translation="MARASASEASRKKRKKTMPTTILICGSPAGGFQIVGLFADRGDA
                     ESYAEDYSLKDWWIAELTSPEAFAESQRQRFRASRGPIPKISI"
     gene            complement(3340..>4286)
                     /locus_tag="VM910_27765"
     CDS             complement(3340..>4286)
                     /locus_tag="VM910_27765"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVI66346.1"
                     /translation="IAYKFHQSMIDAVTGGREVTRQEEPRLYNLLENLCISRGITTPK
                     LKVIEDDSLNAFATGMNDKQYSITVTTGLINALNNAEIESVLGHELTHIRNGDVRMLV
                     IAVIIAGVIGFFAELVFRLLFQGGFSWRGGRGGGDRKGAGMAILIAFVLIAVAWILSI
                     VIRFALSRKREYLADAGSVELTKNPDAMISALRKIEGRGELPGATSAVMEMCIDNPRE
                     GFSNIFDTHPPVDARVAALVKFAGGHDPGPLALPAPSEAQAGESEDADQPTAAGPWGG
                     SQPAPGSGAPFLPSRPPVELGSPPSADGEPGPWGPHRK"
BASE COUNT          777 a         1376 c         1362 g          771 t
ORIGIN      
        1 tgggcgggga tgaagatcga caaggtcgcc ggcatcgagg cgcgcggctt catcctcggc
       61 ggggcggtgg cgcaccaggt ctcggccggc ttcgtgccga tccgcaagaa gggcaagctg
      121 ccgcacaagc gggtgtcgat cgcctattcg ctagaatacg gcctcgacga gatggagatg
      181 cacgaggacg cggcggagaa gggcgaccgg gtcatcctcg tcgacgatct gatcgccacc
      241 ggcgggacgg cggagggcgc ggtcaagctg ttgcgccaat tgggcgcgca ggtgctcgcc
      301 gcctgcttca tcatcgacct gcccgacctc ggcggcgcag cgaggctgcg caagctcgac
      361 gtgccggtgc gcacgctcat ttccttcgaa gggcactagg tcgcgtatcc accgcagcgc
      421 ggcgtgaggt agcttttgac aacgcaactg tgactgcgca ccactcgagg gacgatcgtg
      481 aaacctgccc ggcgacgatt tctgcatctg gccgttggcg ctgctgcgct gccggcactg
      541 ccacggatca caagggcgca aagctatccg tcgcggctcg tcacgatcat cgtgccgttc
      601 gccgccggcg ggacgaccga cgtcattgcc cgcatcctcg gcgagcacat gtcgcgcacg
      661 ctcggccagc agttcatcgt cgagaacgtc gtcggggcgg gcggcaccac cggctcgacg
      721 cgtgcgatgc gcgcaaaccc cgatggatac accattgaat tgggcaatat gggaacgcac
      781 gcggcttccg tggcgctcta ccccagcctt gcctacaatc cggaggtcga ttttgcgccg
      841 atcggcatgg tcgccggtct tccggtcctg gtggtgggga aaaaggatct tccggcgaac
      901 gacctgaagg aattcgcaac ctatgtgaga gcgaatgccg agaagttgaa catggcgcat
      961 gccggtgtcg gttcgatcac gcatttcact tgcctgctgc tcaattcgat actcggcgtg
     1021 aaaccgaccc tggtgccctt caacggcgcg gcgcccgcca tcagcgcgct gatcggcggc
     1081 caagtcgatt acatgtgcaa tgtcgccccc gacgtcgttc agcagatcca gggcggcgca
     1141 ttcaaggcct atgcaatcgg aggctccaag cgcaattcgg cgctgccgga cgtgccgacc
     1201 tcggaggagg ccgggcttcc gcaattccag gcatcggcct ggaacgcctt gtttgcgccc
     1261 aaggggacac caaaaccgat cctcgacaag ctcagcgacg cgctcgacaa ggcgatcgat
     1321 gacgagaaca cacggcagcg cctactcggc ctcggcagcg aaattccgga caaagcccgg
     1381 cgcggtcagc agccgctcgc cgcgctggta aaagacgaga tcgcgcgctg gacgccgatc
     1441 atcaaagccg cggccgtcaa gtcggaataa gcaggctcgt ggcccgcatc ttcagcgccg
     1501 ggaccaccat gaagcttccc cgccgcaaag tcttgcagct ggccgcgggc gctgccgcgc
     1561 tggtggccgt ggcgcctatg gcgcgtgcgc aagcctatcc gacgcggccg gtgcgcattg
     1621 tcgtcgggtt cgcggccggc ggttctaccg acatcggcgc gcgcttgatc ggacaatggc
     1681 tgcaggagcg gctcggccag ccattcgtta tcgagaaccg gccgggcgcc ggcaccaata
     1741 tcgcgaccga gatggtcgtg cgcgcgcccc ccgacggcta tacgctgctc atggtcggcc
     1801 cgtcgagcgc ggtcaacgcg acgctctacg acaagcttga tttcgtgttt ctgcgcgaca
     1861 tcgcgccggt tgcgagcttg atccgacaac cgcaaatcat gctggccaat ccgtcggtca
     1921 tgagcaaaac cgttcccgag ctgatcgccg acgccaaggc caatccgggc aagatcacga
     1981 tggcctcggc tggcgtaggt tcggccggtc atttggtcgg cgaattattc aagatgatgg
     2041 ccggcgtcga ttttgttcac gtgccctatc gtggtgcggg gcccgcattg gctgatcttc
     2101 tcggcgggca ggtcatgatc tcgttcgccg ggatcgccgg atcgatcgcg tatatcagga
     2161 ccggcaagct gcgcgcgctc gcggtgacga ccgcaacgcg gtcggaggcg ctgccggacg
     2221 tcccgaccgt gagcgaattc gtgcctggct acgaggccgg cgactggctg ggggtcggcg
     2281 cgcccaaaca cacgcctgcc gcgatcgttg ataagctcaa cggcgagatc gcgaccgccc
     2341 tcgcgatccc aagatcaagg cgcgctttgc cgatctcggt ggcaccgcgc ttgcgctcac
     2401 gccagccgag ttcggcaagc tcctcgccga cgaaacgcag aaatgggcca aagtgatccg
     2461 ggccgcccat atcaagccgg aatgatgtcg gtcgtcagcg ccgagttgtc tatccgtcag
     2521 tcgtcagatc gaaattttcg ggatcggacc gcgagatgct cgaaaccgtt gccgctggct
     2581 ttccgcgaac gcttccggtg atgtcagctc ggcaatccac cagtctttga ggctataatc
     2641 ttcggcatag gattcagcat cgccgcgatc ggcgaacaag ccgacaatct ggaagccacc
     2701 ggcaggactg ccgcatatca ggattgtcgt aggcatggtt ttttttctct tttttcgaga
     2761 tgcttcggag gccgatgcgc gcgccacgct agctcctcca tgctacgtat tccggtgatc
     2821 gaaacagccc gcgccattcc aatcgtcacc atcaatccag atgcatcgtc gactgatcct
     2881 tcatcgtcct gcacacgcgc ctccttgcat cgtcttttta tcaaagggga agtcgatcgg
     2941 gcgcagccag ctacgtcata aggagtcggc gcaaacgcaa ataaaagcgg ctgaaagatc
     3001 ccccagcgcc ggcgagggct tttcgcgcgc ggtgcctcag gccggaaggt atatcgacga
     3061 cagcacaaca agcacgccga gcatcgtgta aagcagcatg cccgtccttt ccgaagccca
     3121 tgtcatgacg tatccccctt cttccacgca ggtttatctc gaagtagggg atatatgagg
     3181 cggcaccgcg tcgtctgtga tcgccatcac ttttttgcgc atttgacccc aatccggcgg
     3241 gcgaaaaggc ctaaccggcc cggccccggc ccgtgccatc agcccacccg gaggctcctt
     3301 gccgctcggg gtcagaagcg gaaaggctca gagtgccggc tatttccggt gcggtcccca
     3361 ggggccgggc tcgccgtcgg ccgacggcgg ggagccgagt tcgaccggcg gccggctcgg
     3421 caggaacggc gcgccgctgc cgggtgccgg ttgcgatccg ccccacggcc cggcggcagt
     3481 aggctgatcc gcatcctcgc tttcgccggc ttgcgcttcg ctcggcgcgg gcaacgcgag
     3541 ggggccggga tcgtggccgc cggcgaattt caccagcgcc gccacgcgcg catcgaccgg
     3601 cgggtgggtg tcgaaaatat tggaaaatcc ctcgcgcgga ttatcgatgc acatttccat
     3661 gaccgccgag gtcgcgcccg gaagctcgcc gcggccctcg atcttgcgca gcgccgagat
     3721 catggcgtct ggattcttgg taagctcgac cgagcccgcg tcggcgagat attcgcgctt
     3781 gcgcgagagc gcgaagcgga tgacgatcga caagatccag gcgaccgcga tgagcacgaa
     3841 ggcgatgagg atcgccatcc ccgcgccttt gcgatcgccg ccgccgcggc cgccacgcca
     3901 gctgaatccg ccctggaaca gcaggcggaa gacgagctcg gcgaaaaacc cgatcactcc
     3961 agcgatgatc accgcgatca ccagcatgcg cacgtcgccg ttgcggatgt gggtcagctc
     4021 gtggccgagc accgactcga tctcggcgtt gttgagcgca ttgatcaggc ccgtggtgac
     4081 cgtgatcgag tattgcttat cgttcatgcc ggtggcgaag gcgttgaggc tgtcatcctc
     4141 gatcaccttg agctttggcg tggtgatgcc gcgcgagatg cagaggttct cgagcaaatt
     4201 atagagccgc ggctcctcct gccgcgtcac ctcgcgcccg ccggtcaccg cgtcgatcat
     4261 gctttgatgg aatttgtagg cgatga
//
LOCUS       DATLJT010001207         1791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3536774,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001207 DATLJT010000000
VERSION     DATLJT010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536774"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..385)
                     /locus_tag="VM910_27770"
     CDS             complement(194..385)
                     /locus_tag="VM910_27770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66347.1"
                     /translation="MTYLFTVIAFFVVGGIAWFARQQHSSTDNIEIGAKQIRHELRVI
                     AYLLAGVVILLGVIADKIQ"
     gene            800..>1791
                     /locus_tag="VM910_27775"
     CDS             800..>1791
                     /locus_tag="VM910_27775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66348.1"
                     /translation="MNRVALLLAATALFTAGRAAAQSASEFYAGRQITFIVGASTGGG
                     YDRQARLVARHLGKHIPGAPTIVVQHMPGAGSLAATNYIYNAAARDGSVIALVMRSML
                     LIKNWNPQSVRFDLQGLNWIGSINSEVAVVAAWHTAPHKSASDLFEQELIVGGTTGVD
                     PETTPRLLNALIGTKFKIVNGYPGTTEIILAMERGELQGIGDWSWSSLKTARPDWLRD
                     NKITLLLQAALHKEPELAGLPSALDFVNNDADRKVMELYLTQKTLARPLIAPPGIAPE
                     RLTALKAGFAALAHDNDFLADARRAGIDVSPLAGEAVDRIIAMISSAPPETAARL"
BASE COUNT          360 a          550 c          546 g          335 t
ORIGIN      
        1 atagaatttg agcaaggcgt cacagcgcct ccgtacttca ccaaggtcgt aatcaactaa
       61 cgccgtccca agtcgcaaac ctccccgagt tcggcctttc agatcgcgag atttgcaagc
      121 gtgcgacagt aagcgcgcag acgcggccat tcgcgctcgg aatggcgtcc cgcgagcttt
      181 ggcgactggg cgcttattgt atcttgtccg caatgacgcc gagcaggatc acgaccccag
      241 ccagcaaata ggcgatcact ctcagctcat ggcggatctg ctttgcgcca atctctatgt
      301 tatcggtgct ggaatgttgt tgccgtgcga accacgctat tccccctact acaaaaaaag
      361 cgatgactgt gaagagataa gtcatgacca tatactatgt ggtgctggga tcagaaacgg
      421 aggggcactc gcttcggcgt attcggacaa ccctgggatt cggacgaatc ggggccgtcc
      481 agctcccctt gaccaagatg atttcacatc acccgctgtc atgatgtgat gaccatcaca
      541 ttttggcaca attccgacct tagcagcggg cggacaggtt cgagctggtc aatgccccga
      601 aggccaggat gctcgacctc accgtgctcg caaaggcagc tcgtagcagg ctgccgaggt
      661 gttcggcacg ttggattgag cattcaaagc aatgttctgc gcggatggat ttggaccgtc
      721 agaccctatt gcatgctacc atcctcgagg cagcccgacg tctcgggaca gtgcgagcgt
      781 ggatagattg gggcgcgcga tgaaccgtgt cgcgcttttg ctcgcggcga cggcgctctt
      841 caccgcggga agagccgcgg cgcaatcggc ctcggaattt tacgccggca ggcagatcac
      901 gttcatcgtc ggcgccagca cgggcggcgg ctacgaccga caagcgcgtc tggtggcgcg
      961 ccaccttggc aaacatatcc cgggcgctcc gaccatcgtc gtgcagcaca tgccgggcgc
     1021 gggcagtctc gcggcgacca attacatcta caatgccgcg gccagggacg gctccgtcat
     1081 cgccctcgtg atgcggagca tgttgctgat taagaactgg aacccgcaat cggtacgctt
     1141 cgatctccaa ggtctcaact ggatcggaag tatcaacagc gaagtggccg ttgtggcggc
     1201 gtggcacacc gcgccgcaca agtcggccag cgaccttttc gagcaggagt tgatcgtcgg
     1261 cggcaccacc ggcgtcgatc cggagacgac gccgcggctc ctgaacgcgc tcatcggcac
     1321 aaagttcaag atcgtcaacg gctatcccgg caccaccgaa atcattctgg cgatggagcg
     1381 gggagaattg cagggcatcg gcgattggtc gtggtcgagc ctgaagaccg cgcggccgga
     1441 ttggctgcgc gacaacaaaa tcacgctgct gctgcaggcc gccctgcata aagagccgga
     1501 gcttgccggc cttccgtccg cgctcgactt cgtcaacaac gatgccgacc gcaaggtgat
     1561 ggaactttat cttacgcaga agactttggc gcggccgctc atcgcgccgc cgggcatcgc
     1621 gcccgagcgg ttgacggcgc tcaaggcggg cttcgcggcg ctggcgcacg acaacgattt
     1681 cctggccgat gcccgcaggg cggggatcga cgtctcgccg ctcgcgggcg aggcggtcga
     1741 caggatcatc gcaatgattt cgtccgcccc gcccgagacg gccgcgcggc t
//
LOCUS       DATLJT010001208         3760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1698899,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001208 DATLJT010000000
VERSION     DATLJT010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698899"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VM910_27780"
     CDS             complement(<1..253)
                     /locus_tag="VM910_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017962347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HVI66349.1"
                     /translation="MLAGVVPSAVQYVPRQAWRNGSALLGERTLAEEVPVAFSYDGMA
                     HAVLMATPDDLEDFALGFSHTEGIITAPAEIAELAVVSVA"
     gene            complement(256..2607)
                     /locus_tag="VM910_27785"
     CDS             complement(256..2607)
                     /locus_tag="VM910_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HVI66350.1"
                     /translation="MSDQSPPPSGQTEPHQEPIGKPVFAPYDAPAGGWGALHATARAL
                     REQSVEIRGSRALLSMNQPDGFDCPGCAWPDPRHTSSFDFCENGAKAVTWELTKHRVT
                     REFFASHTVRELEQESDYWLEQQGRLTEPMRYDSTTDHYVPVDWDEAFATIGRELNAL
                     DDPNEAEFYTSGRTSNEGAFMYALFARRFGTNNFPDCSNMCHEPTSVGLPESIGIGKG
                     TVLLDDFAYSDAIFIIGQQPGTNSPRMLTELHNASRRKVPIVVFNPLRERALERFAAP
                     QDPVEMATFGETRIASEYCQVRVAGDVAALKGVMKLVLEAHDASLQDGREPILDVEFI
                     AGDTHGFEAFADDLRHTSWEDILRVSGLPRDQLQRVANIYMRAQAVIVCYGMGITQHR
                     LGTANVQQTVNLLLLRGNIGKPGAGICPVRGHSNVQGDRTMGIDENPKPEFLDQIKKV
                     FGFDPPRARGHRVVDAVQAMVDGRARVFIGLGGNFVRAVPDWPAAEAAMRRLKLTVAV
                     STKLNRGHLVHGEQALILPVLARSDIDMQLGGRQSITVEDSMSMVHASHGLVDPPSEH
                     LKSEVAIVCGMAKATLPDCGIDWDSFVADYNRIRDKIEEVFPQLFADFNERIRHPGGF
                     HLTTPPRQRVWETATGRANFLVFPGVDEDRPVSDPAMLRLATLRSHDQYNTTIYTLDD
                     RYRGVFGGRMVVFMNETDMRARGIAEGDLVEIESLADDKRRVVGGFWAKPHSIAAGSV
                     GAYYPETNPLLPLTFHDEKSGTPAAKSIPVLVRRHAPQLNPGD"
     gene            complement(2639..>3760)
                     /locus_tag="VM910_27790"
     CDS             complement(2639..>3760)
                     /locus_tag="VM910_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI66351.1"
                     /translation="GRVTILCGSGCAGAHSELRELGERLKAPMVHALRGKEHVEWDNP
                     YDVGMTGLIGFSSGYYAMLDCDVLLMLGTDFPYRQFYPQDSGVRIAQVDIRSENIGRR
                     AAVDFGVVGDIKATLTALLPLLQQKRDDTHLAQAREHYAKARKALDDLAAPGRGLIHP
                     QQIAKAISDHAATDAVFTCDVGLPTVWAARYLAMNGKRRLIGSFWHGSMANAMAQAIG
                     AQAAFPSRQVISLSGDGGFTMLMGDFLSLVQLGLPVKVVVFNNGALGFIELEQKSTGF
                     LPFGTEFKNPNFAAMAEAVGMRGIRLEDPGEVETGIAAALNHNGPVLVDAVVNRTELA
                     MPPAVTLEMAKGFTLYMVKAVMSGRGDELLDLARSNLWR"
BASE COUNT          714 a         1175 c         1146 g          725 t
ORIGIN      
        1 cggcaacact gacgacggcc agttccgcaa tttcggccgg cgctgtgata attccttcgg
       61 tgtgactgaa gcccaacgca aaatcttcga ggtcgtcggg tgtcgccatt aacaccgcat
      121 gagccatacc gtcataactg aatgcgacgg ggacttcctc ggccaaggta cgttcgccca
      181 gcagcgcaga gccgttacgc catgcttggc gtgggacgta ctgcaccgcc gacggcacca
      241 cacccgcgag catcgtcaat ctcccggatt gagctgcggc gcatggcgac gaaccaagac
      301 cgggatcgac tttgctgccg gagtgccgct cttctcgtca tggaaggtga ggggcagcaa
      361 cggatttgtt tccgggtaat aagcgccgac tgagccagct gcgatgctgt ggggctttgc
      421 ccagaagccg ccaaccactc ggcgcttgtc atcagccagg gattcgattt cgaccaagtc
      481 gccctcggcg atgccgcggg cgcgcatgtc cgtttcattc atgaacacga ccatgcggcc
      541 gccgaacacc ccgcgatacc ggtcatcgag agtgtagatt gtcgtattgt actgatcgtg
      601 gcttctcagt gtcgccagcc gaagcatcgc cgggtcggag actgggcgat cctcgtcgac
      661 cccgggaaac accagaaaat tggcgcggcc agtggcggtc tcccagaccc gctggcgtgg
      721 cggagtggtc aggtgaaagc cgcccgggtg acggatgcgc tcgttgaaat cggcaaacag
      781 ctggggaaag acctcttcga ttttgtcgcg aatccgattg tagtcggcga caaaggagtc
      841 ccagtcgatg ccgcaatcgg gcaaggtggc ctttgccatg ccgcagacaa tcgcaacctc
      901 cgatttcagg tgctcacttg gcggatcgac gagaccatgc gacgcatgga ccatcgacat
      961 gctgtcttcg accgtgatcg actggcgacc gcccagttgc atgtcgatat cgctgcgcgc
     1021 cagaaccgga aggatcagcg cctgttcgcc gtggaccaga tgaccgcggt tgagcttggt
     1081 gctgacagca acggtcagct tcagccgccg catcgccgct tcggccgccg gccagtcggg
     1141 aaccgctctg acgaaattac cgccgagccc gatgaaaacc ctggcgcgtc cgtcgaccat
     1201 cgcctggacc gcgtcgacga cacggtgccc gcgcgcccgc ggcggatcaa aaccgaatac
     1261 tttctttatc tgatcgagaa attccggctt cgggttttcg tcgatgccca tcgtgcggtc
     1321 gccttgcacg ttcgaatggc cgcgcacagg acagatcccc gccccaggtt tgccgatatt
     1381 gccgcgcaat agcaacaggt tcacagtttg ctgcacattt gcggtgccga gccgatgctg
     1441 cgttatcccc ataccgtagc agacaatgac ggcttgcgcc cgcatgtaga tgttcgccac
     1501 acgctgcaat tggtcgcgcg gcaagccgct gaccctcagt atgtcttccc aactcgtgtg
     1561 gcggagatca tcggcgaagg cctcgaagcc gtgggtgtcc cccgcgatga actcgacatc
     1621 gagtatcggt tcgcggccgt cttgaagcga cgcgtcatgc gcctccagca ccagcttcat
     1681 cactcctttg agtgccgcga catcgccggc gacgcggacc tggcaatatt cgctggcgat
     1741 tctggtctcg ccaaacgtcg ccatttccac cgggtcctga ggtgccgcaa acctctctag
     1801 cgcgcgttcg cgcaacggat tgaacaccac gatcggcact ttgcggcgcg aagcgttgtg
     1861 caactccgtc aacatgcgcg ggctgttggt gccgggctgc tggccgatga tgaagattgc
     1921 gtctgaatac gcaaagtcat ccagcagtac ggtccctttg ccgatgccga tgctctcagg
     1981 aagaccgaca ctggtcggct cgtggcacat gttcgagcag tcgggaaaat tgttggtacc
     2041 gaagcggcgc gcaaagagcg cgtacatgaa cgccccctcg ttcgaggtac gtcccgacgt
     2101 gtagaattcc gcctcgttgg ggtcgtcaag cgcgttgagt tcgcggccaa tggtggcgaa
     2161 cgcttcatcc caatcgaccg gcacgtaatg gtcggtcgtg gaatcgtagc gcatcggctc
     2221 cgttaggcgc ccctgctgct ccagccagta gtcgctttcc tgttccaatt cgcggacggt
     2281 gtgggacgcg aagaactcgc gcgtcacccg atgcttggtc agctcccagg tgactgcctt
     2341 ggcgccattt tcgcagaagt cgaacgaact ggtgtgcctc gggtctggcc atgcgcagcc
     2401 ggggcagtca aaaccatccg gctggttcat cgacaacaat gctctggagc ctctgatctc
     2461 gacgctctgc tcgcgcagcg ctctggcagt agcgtgcagg gcgccccagc caccggcggg
     2521 agcgtcatag ggtgcaaaca ccggcttgcc gataggctcc tggtgaggct cggtctgacc
     2581 ggaaggagga ggagactgat cgctcatgat cgggaactcc gaggtgggtt cataagggtt
     2641 agcgccacaa attcgaacgc gccagatcga gcagttcgtc gccgcgcccg ctcatcactg
     2701 ccttcaccat gtagagcgtg aagcccttgg ccatctccaa ggtgaccgcg ggcggcatcg
     2761 caagttcggt gcggttcacg accgcatcga ccagaaccgg cccgttgtgg tttagtgcgg
     2821 cagcgatgcc ggtctcgacc tcgcccgggt cttcgagcct gatcccgcgc atgccgacag
     2881 cttcagccat tgccgcgaag ttcgggttct tgaactcggt gccgaatggg agaaaaccag
     2941 ttgatttctg ctccagctcg ataaagccga gcgcaccgtt gttgaacacg accaccttca
     3001 ccggcaggcc gagctgaacg aggctgagaa agtcgcccat cagcatcgtg aacccgccat
     3061 cgcccgacag cgaaatcact tggcggctgg ggaacgccgc ctgggcgccg atcgcctggg
     3121 ccatcgcatt agccatcgag ccatgccaga aagacccgat cagtcggcgc ttgccgttca
     3181 tcgccaggta gcgagcggcc cacaccgtcg gcaggccgac gtcacaggtg aagacggcgt
     3241 cggttgcggc atggtcactg atcgccttcg cgatctgctg gggatggatc agccctctgc
     3301 cgggggcggc caagtcgtcg agagccttgc gcgctttggc gtaatgctcg cgcgcttgcg
     3361 ccaaatgcgt gtcgtcgcgt ttctgctgca agagcggcag caaggcagtg agcgttgcct
     3421 tgatgtcgcc gaccacgccg aaatcgaccg cggcgcggcg tccaatgttc tcagaccgga
     3481 tgtcgacttg ggcgatgcgc acgccgctgt cttgcggata aaactggcga tagggaaaat
     3541 cggtcccgag catcagcagc acgtcgcagt cgagcattgc gtaatagcct gacgagaagc
     3601 cgatcagccc ggtcatgccg acatcgtaag ggttgtccca ttcgacgtgt tccttgccgc
     3661 gcagcgcgtg caccatcggt gctttgagcc gctcgccgag ctctcgaagc tcgctgtgcg
     3721 caccggcaca gccggagccg cataaaatcg tgacccgccc
//
LOCUS       DATLJT010001209        20189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4455951,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001209 DATLJT010000000
VERSION     DATLJT010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20189
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4455951"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VM910_27795"
     CDS             <1..783
                     /locus_tag="VM910_27795"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI66352.1"
                     /translation="KFALKLFVDRDCAGTERDLPSGSRVIYKITEKRLGLSSDKASSF
                     FATALVGNMTMTGQWSRDASTPPFIDLVPIEQATGARKEFYDEMERLRGSGRVSNLFK
                     AYAAFPALAKANFQRLMVLLNQGTLSVKFKEALMTALAEANKCDYCVSFHATALRNAG
                     ASDEEVVAALEFDPDRIGLSGKEKALFNYALKANGDPHSIVAADIEELRRKGASDWDL
                     IETLETVNTGNSFNLINGALNVGPDDFLNYMKGRGELWKKSA"
     gene            794..1156
                     /locus_tag="VM910_27800"
     CDS             794..1156
                     /locus_tag="VM910_27800"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVI66353.1"
                     /translation="MATLSELTSAERVDAALAAPGLLVLDVYVQSCIICRRIEPMVAA
                     VAVASGGAVRAHKLDAERHAEFAAKYDIRGVPTLLLFRDGRLISRRSGFSTATALRGW
                     IGANARDEAWGRSDYART"
     gene            1231..1533
                     /locus_tag="VM910_27805"
     CDS             1231..1533
                     /locus_tag="VM910_27805"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HVI66354.1"
                     /translation="MYRGWGLDPKTGEVTSDDVIEQTRQTIVNLEAILAAAGCTLQHV
                     VKATVYLTDMADYPRVNKVYDEAFAPNKPARTCVAVSALPVRERMKIDLVDYPEGN"
     gene            1686..2954
                     /locus_tag="VM910_27810"
     CDS             1686..2954
                     /locus_tag="VM910_27810"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66355.1"
                     /translation="MRNGVRRVAGAMALVAAYLQFGPALAQSKPSAIKIAIVQFLSGA
                     GAPHDASAVNTARLLTEQFNAAGGIEGVRLNTMYVDEAGSATDKVAEYRRLVQDEKIT
                     VAIGYTSSANCLAVAPVADELKTLTIFHVCANYRLIDKGVHRYVFRTAAHAASENISA
                     ALYVLATKPDLKTIAGLNYDYAYGRDSWDVFKRTILKLKPDVQVAGELWTKFLATEYS
                     AEISRLLALKPDVIHTVNWGAGLTSLINQGETRGLFEQSLVMMTTGLLNQAEILPKGA
                     AFSGRGYHLQYPDPDKNAGNREYLRSYKAKFNSMPDYQGTFMAQAFYGLKAAIEKAAK
                     VKNGAWPTTEEIAAAMEGLEFDTPRGRVLVRKDHEAVHEAMWGLTSGQKHPAFGYPIL
                     DQFRVFPAADVTPPEGKKTVEWIESWPTRK"
     gene            3118..4014
                     /locus_tag="VM910_27815"
     CDS             3118..4014
                     /locus_tag="VM910_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66356.1"
                     /translation="MNILPIILIDGITFASWLFLVSAGITFIFGVLRILNVAHGSLYA
                     IGAYLGATFILQFAGREQHGWITLLLLLLAATVVGLTLGPLIERLFLRRVYGEDEVIG
                     LLTTFALFLILEDVVRLIWGIEPYLVEGPFRLFGRVRVAGIAFAGYPFLLTIVAILAG
                     LLLWFVIHHTSFGREVTCVINDREMAGALGINVPRIDLMAFALGTFLAALGGAFTAPM
                     MSVEPGMGVSVIVLAFAVVGIGGLGSIGGAALGCIIVGIARASSVQLFPEIELFVIYA
                     LMVAVLLWRPQGLFGAIELRKI"
     gene            4011..4961
                     /locus_tag="VM910_27820"
     CDS             4011..4961
                     /locus_tag="VM910_27820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66357.1"
                     /translation="MSEPETHPSLPLAAIVAFAVMAILPPFLSDWITFLIALALAKGA
                     VVLGVVLLLRGGLISFGHALYFAIGAYAVGFLTNRFGIGEALVGLPLATIISALVAAA
                     LGLLLMRFRGIYFAMLSLALSMILYTVLIKFYDFSGGTDGLSVRGFTLAGAAPAHPGL
                     VQYYLVLVLTAAVVYVGARFLASPLGYLMQAVRYNEIRIEYLGASASHAILGSYVVSG
                     AISGLGGALVAYLVGHVSPELAFWVSSGDFVFVAVLGGTGSVFAPLIGSIAFEFVKNY
                     ALKFSPNTWQLTLGVFLLVVIYFQPQGLWGLLTRLRARPR"
     gene            4970..5707
                     /locus_tag="VM910_27825"
     CDS             4970..5707
                     /locus_tag="VM910_27825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66358.1"
                     /translation="MIDVQNVSRAFGMLRAVDDVSLSVGEGETVGIIGTNGSGKTTLL
                     NLITGYLQPTSGRIVFQGTDITGLGPRRVTALGMARSFQIPQLYTGLTVLDNILIAIA
                     AHSGRSADFWTPMYRSDCVTKADELLALFGLRDQAAATVSDLPEGGRKVLDVALSVIL
                     EPKVLLMDEPTSGVSARDKFSVMDTLMPALKRGRVTTIFVEHDLDIMERYAERAVAFD
                     GGKIIADGPVADVLARPEVRRAVLGES"
     gene            5709..6383
                     /locus_tag="VM910_27830"
     CDS             5709..6383
                     /locus_tag="VM910_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI66359.1"
                     /translation="MLRIEGITVEISGVTILRGLSLAVETGATVGLVGRNGAGKTTTL
                     RSVMGLTHLSSGRINLDGNKLDGMSSYMRARFGIGYLPEDRRLVGALSVRENLLIPAQ
                     ASGVPNYEERLHRIFDLMPELREWSARRAITLSGGQQKMVALARAFVNGRKLLLLDEP
                     FEGISTALSRRLAQTIQDFQRAEQGVTVLVAESDLKRAAMLTRRAFVIERGEVIEEAV
                     FADMDS"
     gene            complement(6441..6932)
                     /locus_tag="VM910_27835"
     CDS             complement(6441..6932)
                     /locus_tag="VM910_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HVI66360.1"
                     /translation="MIFPHTALDRAAQALQLASTQAVRIVTAETCTAGLVSSCLTSVP
                     GASKTFERGFVLYHDSAKATGLGVPEGVSRQYGAVSAEVTKGLAEGALAHSTASVSVA
                     VTGYAGPGGGNEKNPVGTVYVAAARKGAETLVERHVFSGDRDAVRIQAVGAALTLLVR
                     RLS"
     gene            complement(6932..7819)
                     /gene="nadC"
                     /locus_tag="VM910_27840"
     CDS             complement(6932..7819)
                     /gene="nadC"
                     /locus_tag="VM910_27840"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVI66361.1"
                     /translation="MQLNRFMIRPYVEQALRDEIGPGDTTGGFLVGEDPVQTAQIYAK
                     GSGIVCGLLLADETIRTVDPDAQIEHMVKDGEEMGPGKVLLKVEAKASTFFMIERCAL
                     DWIQQMSGIATKTRRYVNLVKHTNVRITDTRKGWPGLRMLQKYAVRCGGAHNHIFSLS
                     NCVLVKDNHIKIAGGIRNAVEILRSRAQHTFKLEVECETLDMVQEALDCGVEVIMFDN
                     MDLDEIKAGLKLVNGRAITEASGGINERTIAIIAETGVDVISVGDLTHSVTSVDISLD
                     VKDIKPSAIRAIERLRAAG"
     gene            complement(7886..9154)
                     /locus_tag="VM910_27845"
     CDS             complement(7886..9154)
                     /locus_tag="VM910_27845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66362.1"
                     /translation="MAVKNQTTAIIGARVIDGLGRDPIEKGTVVIEGGRISAVGKDTA
                     VQVPHGANVLDAAGRTVLPGIIDCHVHSTYRARDIRQHLTNTPTYNVLRSTHILEETL
                     ACGVTTARDMGGADAGFREAIAEGFIKGPRLLISIVMISQTGGHGDSWVPAGIRLQKR
                     AWLPSGVVDGPEEMRKLVRTLLMAGADFIKICTTGGITSLADSWDEPQLAPDEIRTAV
                     AEARAKRKGVAVHAEGAEGIRNALTADIHSLEHGWFLDETCVDHMIKHGIWWVPTIAL
                     VPLSVERRKVDPAWSNQQLAKEDEKDAAIYAAMQKQIPLWKDAVRRGIRVAMGTDQSH
                     RLLVGENLVELEYMVKWLGMPEMDAIVAATSRAAECIERPELGALAPGKRADVLAVDG
                     DPLADIAVLQDRKRLHLILKDGWAYKNLIN"
     gene            complement(9275..10735)
                     /locus_tag="VM910_27850"
     CDS             complement(9275..10735)
                     /locus_tag="VM910_27850"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI66363.1"
                     /translation="MIELETKLLIDGQFVAGAASNPFTVISPITGQAYAGVARAGPEE
                     LDRAAAAARRAFDEGPWPRLAPFERGRMLRAIADRIRSEVDIIAEVETRSGGKTITDS
                     RNEIEAAASVFDYYAGAMDKYFGETIPMGQGVLDFTLREPVGVVALITPWNFPFLAAA
                     WKVAPSLVTGCTVILKPASYTPLTSLLLGRIALDAGVPDGVLNVLPGPGAALGERIAT
                     NPLIDKIAFTGETRTGANLLKAAADGIKRVSLELGGKSPNIVFADGDIAKAAVAAVRG
                     GFGNAGQSCSARSRLLVERKVHDVFLAAFASATEKYRVGDPLDPKTEMGPLVANAQWR
                     NVKDLVDAGLEEGACIVSGGNRPAQWPDGCYFAPTIMTQVDNRMRIAREEIFGPVVVV
                     IPFDNEDEAIGLANDSDYGLNSSVWTRDIGRALRVARALRVGMVSINSHGSASRYGTY
                     APFGGYKKSGVGRELGMHALALYTEVKNVFVELGNR"
     gene            complement(10732..11505)
                     /locus_tag="VM910_27855"
     CDS             complement(10732..11505)
                     /locus_tag="VM910_27855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI66364.1"
                     /translation="MTPIKAVLFDWGETLVRIPGMIHSPERHLGCLKRLFEECGGENG
                     GPDLRALGVSWPSLRTAYIKACRKHMAHSATTRREHRFEDRFATTLRLLQIEQLPEMA
                     VLSRWVDQFGELIAQDAQMVDGADEVTEELARHVRLAIVSNYPHAPVVYGTLERLGLR
                     RHFCAVVVSSELGWLKPHPELYRTALTRIGARAEETVFVGDNLDNDIRAPKALGLRTA
                     WLTTEPSKQPPEADITLSDLRQLTAWCNLPSEGRRERER"
     gene            complement(11521..12327)
                     /locus_tag="VM910_27860"
     CDS             complement(11521..12327)
                     /locus_tag="VM910_27860"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HVI66365.1"
                     /translation="MSVGADFPRLDESCRLADGRLAQAAIARWYDMRLRATHGILIAL
                     DMESLDQMRKVVEQTSGISGVVGYKMGLTVTLRLGLGGAVRHLRASTDLPLIYDHQKA
                     GPDVPDMAQKFSATCKEAKVDGVILFPLAGPRAVVAFAGSAYFNRLLPIVGGDLPFPD
                     YNATGGGYVIDDALDKIFRKAIEIGVDHFVVPGNTPDKVRHHARWLVEKIERPCLFVP
                     GIGTLGGSIADAFAAAPGCNVYAVVGRAIYDAPDPAEAARRLAGEAQRFG"
     gene            complement(12807..13613)
                     /locus_tag="VM910_27865"
     CDS             complement(12807..13613)
                     /locus_tag="VM910_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI66366.1"
                     /translation="MHSPATSPDSRLQDLDGFRVEISAERERADIILDRPPFNTISMP
                     QRNQLRQVFEALDEDPRVRIIVLRSVGEHFSSGGFIRGFLEASPEHVSKLAWNIAAPA
                     RCSKPVIAANRGYAFGVGFEISLACDFRIVSETCLYALPEQKLGQIPGSGGSARLQKI
                     VGITRTKDIVMRSKRISGRQALEWGIATDCVADDKLEATVDALVDELRTFSPLAQRTA
                     KKLLNDAEDATLSIAIELEGLNYSRLRQSDDFREGVEAFHDKRTPKFRGT"
     gene            complement(13617..15182)
                     /locus_tag="VM910_27870"
     CDS             complement(13617..15182)
                     /locus_tag="VM910_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI66367.1"
                     /translation="MLDLGTSFLASVSRDPKAVAVVDGDIRLSYRDWYAKISAVVAGF
                     AELRLAAGDRLVTVLQNRWEAATLHWACQFAGVVITPINWRAKVDEIDYAVQNSGAKA
                     IAFEQVSAENVADSGACHSLARISVAQKSASSDTSFTELVAADAPPAAPRVDADAISL
                     MLYTSGTTAKPKGVPRSHRAERAAAVAHVAQNLYGHGERTLGVMPLYHTMGVRSLLSM
                     SLIGGRFVCLPRFDIVEALRLIEKERISNLYLVPTLYHDIVHHEAFARSDVSSVRKLG
                     FAGAPMTDGLLAKLSDAFRPDLFVNHYGSTEIYTFTINQDAARKPGSAGRAGINQMIR
                     IVKLGASNVDEIAAPGEEGEIAALACGDEAFEGYWQRPEATAQAFRQGWYMTGDTGYA
                     DEDGDIYVTGRVDDMIITGGENVSPVEIESCLSLHPAVAEVAVVGLPDERWGKIVAAF
                     IRRRSDVAHDELDSHCRTSGLANFKRPRRYIFVREIPKSPVGKLLRRKLVVGEYEAEP
                     RPTAVFISSNQTA"
     gene            complement(15175..15684)
                     /locus_tag="VM910_27875"
     CDS             complement(15175..15684)
                     /locus_tag="VM910_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI66368.1"
                     /translation="MPKLNPDRRHQVSFTLNGKATSMEVESRMLLCDALRHIVGATGT
                     HVGCEHGVCGACTIIVDGLPARSCLMLAVQAQGCDIRTVEGLAPSPDRLSVLQAAFRR
                     HHALQCGFCTAGILMSLHAYLSTSLRPSEEELKEVIGGHLCRCTGYAPILAAALEAAA
                     TLRRESLHA"
     gene            complement(15672..16499)
                     /locus_tag="VM910_27880"
     CDS             complement(15672..16499)
                     /locus_tag="VM910_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI66369.1"
                     /translation="MKPPAFDYICARTVDEITATLQEHGSEARVLAGGQSLIAMLNMR
                     LARPALLVDLMRVEPLAQIIEETDAITIPAAVRQSAAMAHPALKRDVPLLAAALPWVG
                     HLQTRSRGTICGSVAHADPSAEIPLCLVALEGEVRLLARRKRRRVTAEAFFTGMMATD
                     REDDELVEAVAYPKRRPGAGYAFIEVGRRHGDFAIVACAAVVDAERMRLAIAGVADRP
                     TARDFPLLEGTALDDALNAFAWELRGSDDIHATARYRRELVRKLGRKVLLEAAACRS"
     gene            complement(16496..19444)
                     /locus_tag="VM910_27885"
     CDS             complement(16496..19444)
                     /locus_tag="VM910_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI66370.1"
                     /translation="MDRAALVGQSLERIEDAALLSGRGAYMDDLGIKPGTLHAAILRS
                     PHAHADIVSIRTEPARALPGVFAVMSGLDLAAITAPLISGVRAPIEARAIAVERVRYV
                     GEPVAIALASDRYRAEDAIDAIEVEYRQHPVVVDPQNALDAEAPILHEAVGSNLVSDR
                     TFVYGDPDAAFAAAARCVSVTAHYPRNTGSPIETYGVVAHYDPHGDAYEILANFQGPF
                     SLHAVIARALKVPGNRLRLRTPQDSGGSFGVKQGIAPYAVLVAAAARMAGRPVKWVED
                     RLEHLAASVSATNRVSTLKAAVDADGHISALDWDQLEDCGAYLRPPEPATLYRMHGNM
                     TGAYDIRNVRLRNRVVVTNKAPTGLVRGFGGPQVYYPLERLMQRIAVELQLDPLDVIR
                     RNLIPASAFPYHTATGAIYDSGDYARALEVAARDGALDELRRRRDEARAAGRLYGIGY
                     TAAVEPSVSNMGYITTALTAAERRKAGAKDGAQATASITLDPLGSVTVQVASTPQGQG
                     HRTVLSQVVADVFGLSMTEVRVTTDLDTGKDAWSIASGNYSSRFAPAVAGATHLAASR
                     LRDKLSRIAAARLNVRPEDLVFAGGKVVAKTNPDAAISFSRLAAISHWAPGLLPDEMS
                     DTIRETVFWTPPQLTAPTDADEINSSLCHGFIFDFCGVEVDRITGKVRIDRYVTTHDC
                     GRILHPGMVEGQIRGGFAQAVGAAFYEELSYGDDGSFLSGTFADYLLPTVTEVPDLTV
                     LHIESKSPFTPLGSKGVGEGNCMSTPACLANAVADALNLATIDLPLTPAKLAASVSGD
                     EPLPPKGMTAPLRVAKPGDRTMTGAGEVRVQAAPAAVWAMLLDADVLASLIPGSYGVK
                     KFSDTHFVADVTLGVGPVKGRYKAEVCLSDLDPPWSATLTGTVVGALGSGGGNGRVTL
                     TPDCDGGTIIGYTYQAALGGKVAAVGGRLLDGAARVIFKQFFAALARKAGGARPGWTA
                     RLLALIGLRR"
     gene            complement(19671..>20189)
                     /locus_tag="VM910_27890"
     CDS             complement(19671..>20189)
                     /locus_tag="VM910_27890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66371.1"
                     /translation="SVGSPGTGTTPSLSIEMLKLALGLDFVTVPFQGGGPMTQSLVGG
                     HTPIVCAALGNYVNLIKEGKLRALAVTAKKRSAALPDVPTLEDIGIAGQEAETMTGVF
                     VPAGTPKAIVHLLQREISAIVNAPDIKAKLLQAGVEAEGNSTADFAAYVNADIAKWKK
                     VITDGKIPLIGG"
BASE COUNT         3749 a         6310 c         6271 g         3859 t
ORIGIN      
        1 aagtttgcgc tcaagctttt cgtggatcgt gattgtgccg gcacggaacg cgatttgccg
       61 tccgggtccc gcgtgatata caaaattact gagaaacgac ttggtctctc ctcggacaaa
      121 gcgtcatcgt tttttgcaac cgcattagtg gggaacatga ctatgacggg tcaatggagc
      181 cgtgatgcca gtacgccacc gttcatcgat ctcgttccga tcgaacaggc gacaggcgca
      241 cgtaaagagt tctacgatga gatggagcgg ttgcgtggat ccggccgcgt ctcaaatctg
      301 ttcaaggcct atgcagcctt tcccgccctt gccaaagcca atttccagcg cctaatggtg
      361 ttactcaacc aagggactct cagcgtcaag ttcaaggaag cgctgatgac tgcgctcgcc
      421 gaggcgaaca agtgcgacta ttgcgtgtcg ttccatgcga cggccctgcg caatgccgga
      481 gcttcagacg aggaagtcgt ggccgcgctc gaattcgatc ccgatcgcat cggcctttca
      541 ggcaaggaaa aggcactgtt caattatgcg ctgaaggcga acggcgaccc tcactcgatc
      601 gttgcagctg atatcgaaga attgcggcgt aagggcgcgt cggattggga cctaatcgaa
      661 acgcttgaga cggtgaatac cggcaacagc ttcaatctga tcaatggcgc tctcaacgtc
      721 gggccggacg atttcctgaa ctacatgaaa ggtcgcggag aactctggaa gaaatctgcc
      781 tgaggagact gaattggcaa cgctctctga gctgacctcg gcggagcgag tcgatgcggc
      841 ccttgcggcg ccgggactgc tggtgttgga cgtctacgtg caaagttgca taatctgccg
      901 tcggatcgag ccgatggttg cggcggtcgc agtcgcatca ggcggcgcgg ttcgtgccca
      961 caagctcgat gcggaacggc atgcagaatt cgctgccaag tacgacattc gcggcgtccc
     1021 aaccttgctc ctgttccgtg acggacgact gatcagccgc cgatcgggct tttcgacggc
     1081 aaccgctctg cgcggttgga tcggcgcgaa cgcgcgcgat gaagcatggg gacgatcgga
     1141 ttatgcccgc acataggctc gttgacgcac ccaaggctcc gcagccgcct ggcccgtttt
     1201 ctcatgcgac ggtggttgga ccgtgggttt atgtatcggg gatgggggct cgatccaaag
     1261 accggggaag tgacctcgga tgacgtaatc gagcaaacgc gccagaccat cgtcaatctc
     1321 gaagccattc tggcggcggc cggatgtacc ctccagcatg tcgtcaaagc aaccgtgtac
     1381 ttgaccgaca tggctgatta cccgcgcgtc aacaaggtgt atgacgaggc ttttgccccc
     1441 aacaaaccgg cgcgaacctg cgtggccgtg tccgcgctgc cggtgcgcga gcgcatgaaa
     1501 attgacctag tcgactatcc agaaggcaac tagaacgccg cgcgaattgt cacaccacgc
     1561 cgggaagaaa catgtcttca gtgtttgacg ctccgctgcc cctggttcca tgccttggca
     1621 ggaaaaatgg aacggagcat tatacatctg gtaaaacggg aaccggcgcg aacgggagag
     1681 ttatcatgag gaatggcgtg cgcagggtag ccggggcaat ggctctcgtc gcggcgtatc
     1741 tgcagttcgg acccgcgctg gcgcagagca agccaagcgc aattaagatc gcgatcgtcc
     1801 agtttctctc cggggcaggc gcaccgcacg acgcatctgc ggtcaacaca gccagattgc
     1861 tgacggagca gttcaacgcc gctggcggga tcgagggtgt ccgtctcaac accatgtatg
     1921 tcgacgaagc cgggtccgcg accgacaaag ttgcggagta ccgtcggctc gtccaggacg
     1981 aaaagatcac tgttgcaatc ggctatacgt cgagcgccaa ttgtctcgcc gtcgctcctg
     2041 tcgccgacga actcaagacg ctgaccatct ttcacgtctg tgccaactat cggctaatcg
     2101 acaagggagt ccacaggtac gtgttccgca cggccgcgca tgcggcatcc gagaatatca
     2161 gtgcagcgct ctacgtcctg gccacgaagc ccgatctcaa gaccattgcg ggcctgaact
     2221 acgactatgc ctacggccgc gattcctggg acgtgttcaa acggacgatc ctcaagctca
     2281 agccggacgt gcaagtggcg ggcgaactgt ggaccaagtt tctggcgacc gaatattccg
     2341 ccgagatctc ccgcctgctc gcgctaaagc cggatgtgat ccacaccgtg aactggggcg
     2401 ccggcctgac cagcctcatc aatcagggcg agacgcgcgg cctgttcgag cagagcttgg
     2461 tgatgatgac gaccggcctg ctgaaccagg cggagattct tcccaagggc gcggcctttt
     2521 ccggccgcgg ctatcacctc cagtatccgg atccggacaa gaatgccggc aaccgcgagt
     2581 atctcaggag ctacaaggcc aagttcaata gcatgccgga ctaccagggg accttcatgg
     2641 cgcaggcctt ctatgggctg aaggctgcga tcgaaaaggc cgccaaggtg aaaaacggcg
     2701 cttggccgac cactgaggag atcgccgctg cgatggaagg cctcgagttt gacacaccgc
     2761 gcggccgcgt cctcgtgcgc aaggatcatg aggcagtgca cgaggcgatg tggggcctca
     2821 cctccggaca gaagcacccc gcgttcggct atccgatcct cgaccaattc cgtgttttcc
     2881 ccgccgccga tgtcactccg ccggagggga agaagacggt cgagtggatc gaaagctggc
     2941 cgacccggaa atagcgccag cgcccttggg gcgaggaccg tcgtcggacg gcagtgcgtt
     3001 cggcggagcg gatgccggcc ttggcgtccg agcgcgcccc aaccctcagg gcaagcgcac
     3061 atgggaggcc gagcgatgtt ccactaggcg cgaaggggct gctgaggtgc ggttctcatg
     3121 aatatactgc cgatcatcct gattgacggc attacgttcg cgtcttggct gtttctcgtc
     3181 tcggccggca tcaccttcat cttcggtgtg ctgcgcatcc tcaatgtcgc gcatgggagc
     3241 ctctacgcca tcggcgccta tctcggcgcg acattcatcc tccagtttgc aggtcgggag
     3301 cagcacggct ggataactct gctgctgctg ctgctcgccg ccaccgtcgt cggattgacg
     3361 ctcggcccgc tcatcgagcg cctgtttctg cggcgtgtct atggcgagga cgaggtcatc
     3421 ggactgctga cgacctttgc gctcttcctc atcctggagg acgtggttcg gttgatctgg
     3481 ggaatcgagc cgtatcttgt cgagggtccg ttccgcctgt tcggccgggt gcgcgtggcg
     3541 ggcattgcct ttgccggcta tccattcctg ctaacgattg ttgcaatcct tgcgggcctt
     3601 ctgctctggt tcgtcattca ccacacctcg ttcggccgcg aggttacctg cgtcatcaac
     3661 gaccgtgaga tggcaggcgc gcttggcatc aatgtcccgc gcatcgacct catggctttc
     3721 gcacttggca cgtttctggc ggcgctcggc ggcgcattca ccgcgccgat gatgtcggtc
     3781 gagccaggca tgggggtgtc cgtcatcgtg cttgcatttg cggtcgtcgg tatcggcggg
     3841 ttgggcagca tcggcggcgc ggcgctcggc tgtattatcg tcggtattgc gcgcgcctcg
     3901 tccgttcaac tgttccccga gatcgagctg ttcgtgattt acgcgctgat ggttgcggtt
     3961 ctgctgtggc gaccgcaagg cctgttcggc gccatcgagc tgcgcaagat atgagcgagc
     4021 ccgagaccca tccaagcctt cccctggcgg cgatcgtcgc gttcgctgtg atggctatct
     4081 tgccgccgtt cttgtcggat tggatcacct tcctcatcgc gctcgcgctg gccaagggcg
     4141 ccgtcgtcct tggcgtcgtg ctgctcttgc gcggcggcct tatctcgttc ggccatgcgc
     4201 tttacttcgc cattggcgcc tatgcggtcg gctttctgac gaacaggttc ggcatcggcg
     4261 aagcgctcgt cgggcttcca ctggcgacga tcatatcggc gctagtcgcg gccgcgctcg
     4321 ggctgttgct catgcgcttc cgcggcatct attttgcgat gctgagcctt gcgctgtcga
     4381 tgatcctcta cacagtgctg atcaagttct acgatttctc tggcgggacc gatgggctca
     4441 gtgttcgagg tttcaccctc gcgggcgcgg cgcctgcgca tcccggcctc gtacagtact
     4501 acctcgtgct cgtgctcact gctgccgtgg tatatgtcgg cgcgcgattt ctcgcctcgc
     4561 cgctcggcta cctcatgcag gcggtacgct acaacgaaat ccgtattgaa tatctcggcg
     4621 cgtccgcaag ccatgcgatc ctgggatcgt acgtggtctc aggggctatc agcggccttg
     4681 gcggtgctct tgtcgcctac ctcgtcggcc atgtgtcccc ggaactcgcc ttctgggtct
     4741 cctcgggcga tttcgttttc gtcgcggtac tgggcgggac cggcagtgtg ttcgcgcccc
     4801 tgattggttc gatcgcgttc gagttcgtca aaaactatgc gcttaaattc tcgcccaaca
     4861 cctggcagct gaccttgggg gtcttccttt tggtggtgat ctacttccag ccgcaggggt
     4921 tgtggggtct ccttacgcgc ttgcgggcca ggccacggtg accgcagcaa tgatcgacgt
     4981 tcagaacgta tcccgcgcat tcggcatgct ccgcgccgtc gacgacgtga gcctgtcggt
     5041 tggcgagggc gagaccgtcg ggatcatcgg gacaaacggt tccggcaaga ccacgttgct
     5101 gaacctgatc accggctatc ttcagcccac gtccggccgc attgtgtttc aggggactga
     5161 catcacgggg ctcggccccc gacgggtcac tgcgctgggc atggcgcgtt cgtttcagat
     5221 cccacagctc tataccgggc tgacggtgct cgacaacatt ctgatcgcga tcgccgcgca
     5281 ttccggtcga agcgcggatt tctggacacc catgtaccgt agcgattgcg tgaccaaggc
     5341 cgacgaactc ctcgcgctgt tcgggctgcg cgatcaggcg gcggcgaccg tgtcggacct
     5401 gccggaaggc gggcgcaagg tcctcgacgt ggcgctctcg gtgatcctcg agccgaaggt
     5461 actgttgatg gacgagccga ctagcggcgt gagtgcgcgc gacaagtttt ctgtcatgga
     5521 cacgctgatg ccggcgctca agcggggcag agtcaccacg attttcgttg agcacgactt
     5581 ggacattatg gagcgctacg cagagcgggc agtagcattt gatggcggaa aaatcattgc
     5641 cgacggtccg gtcgccgacg ttctagccag gccggaggtt cgccgcgccg ttctcggtga
     5701 gagctaacgt gctccgcatc gagggcatca ccgtcgagat tagcggggta accatcctgc
     5761 gggggctaag cctcgcggtc gagactggcg ccaccgtcgg gctcgttggc cgcaacggcg
     5821 ccggcaagac caccactcta cgaagcgtca tgggtttgac ccacctgagc tcgggccgga
     5881 tcaatctcga cgggaataaa ctcgacggga tgagctccta catgcgggcg cggttcggca
     5941 ttggatactt gcccgaagat cgccgtctgg tgggcgcctt gtcagttcgc gagaacctgt
     6001 tgatcccagc gcaggccagc ggcgtcccga actatgagga aaggctacac cgaatcttcg
     6061 atctaatgcc ggagctgaga gagtggtccg cgcgcagggc gatcacgctg agcggcgggc
     6121 agcaaaagat ggtggcgctg gcccgcgcgt tcgtgaatgg ccgaaagctg cttcttctcg
     6181 atgagccgtt tgaggggatc tcgaccgcat tgtcgcggcg actggcgcaa accatccaag
     6241 acttccagcg cgcggagcag ggcgttaccg tcctcgtcgc cgaatccgac ctcaagcgag
     6301 ccgccatgct gacgcggcgg gcatttgtaa tcgagcgcgg tgaggtgatc gaggaggcgg
     6361 tatttgcaga catggactcg tgaacagatg cctgacgcac gtgttgcctt accgctaagg
     6421 agccgttggg tcacagctca ctacgacaac cgccgtacca atagcgtcag cgcagcgccg
     6481 accgcctgaa tccgtaccgc gtcgcggtcg ccggagaaga cgtgacgttc gacgagcgtc
     6541 tcggcgccct tacgcgctgc agcgacatat acggtgccga cagggttctt ctcgttgccg
     6601 ccgccgggcc cggcatagcc cgtgaccgcc acggagacgc tggcggtcga atgggcgagc
     6661 gccccttcgg cgagcccctt ggtaacttca gcgctcaccg caccatactg ccgcgagacc
     6721 ccctcgggta cgccgagtcc ggttgccttg gcggaatcgt ggtaaagtac gaagccgcgc
     6781 tcgaaggttt tggaggcgcc gggcaccgac gttaggcaac ttgacaccag cccggcagtg
     6841 caagtctccg cggtgacaat gcggaccgcc tgggtggaag cgagctgcag cgcctgcgcg
     6901 gcccgatcca gagcggtgtg agggaatatc atcagcccgc agctcgcagt cgctcgatgg
     6961 cgcggatcgc gctcggctta atgtccttta catcgaggct gatgtcgacc gaagtcacgc
     7021 tgtgggtgag gtcgccgaca gagatcacat cgactcctgt ctcagcaatg atggcgatcg
     7081 ttctctcgtt gataccgccg gaggcttcgg tgatggcgcg accgttcacc agcttaagcc
     7141 cggccttgat ctcgtcgagg tccatgttgt cgaacatgat cacctcgacc ccgcagtcga
     7201 gcgcttcttg caccatatcc agcgtctcgc actctacctc gagcttgaag gtatgctggg
     7261 cgcgcgaccg caggatctcg actgcgttgc ggatgccacc ggcgatcttg atgtggttgt
     7321 ccttcactag aacgcaattg ctcaggctga aaatgtgatt gtgggcaccg ccgcagcgca
     7381 cggcatattt ctgcagcatg cgcagtccgg gccagccctt gcgggtgtcg gtgatacgca
     7441 cgttcgtgtg cttgaccaag ttgacatagc gccgggtctt ggtggcgatc ccggacatct
     7501 gctggatcca gtcgagcgcg cagcgctcga tcatgaagaa ggtagaggcc ttggcttcga
     7561 ccttgagcag caccttgccc ggccccatct cctcgccgtc ttttaccatg tgctcgatct
     7621 gggcatcagg atcgacagtg cggatggtct cgtccgccaa caggagaccg cagactatgc
     7681 cgctgccctt ggcatagatt tgcgcagtct gcaccgggtc ctcacccacc aggaaaccac
     7741 cggtggtgtc gcccggaccg atctcgtcgc gcagcgcctg ctcgacatag ggccggatca
     7801 tgaagcgatt gagttgcatg tgcgcatctc ctcgtccatg ggggtcgcgc tattcgccta
     7861 cgtgcgccag cgcgtcatcg tcagtttagt taatcagatt tttgtaggcc cagccatcct
     7921 tgagaatgag gtgcaggcgc ttgcggtcct gcagcacggc aatgtcggcg agcggatcgc
     7981 cgtcgaccgc cagcacgtcc gcgcgcttgc ccggcgccag cgcgccgagt tcgggacgct
     8041 cgatgcattc ggcagcgcgg ctggtcgcgg cgacgatcgc atccatttcc ggcatgccga
     8101 gccatttcac catgtattcc agctccacca agttctcacc gaccagcaat cgatgtgact
     8161 ggtcggtgcc catcgctacc ctgatcccgc gtcggaccgc gtccttccac agtgggatct
     8221 gcttctgcat cgcggcgtaa atggcagcgt ccttctcgtc ttccttggcg agctgttgat
     8281 tgctccaggc ggggtcgact tttcgccgct ccactgacaa tggcacaagt gcaatggtcg
     8341 gcacccacca gatgccgtgc ttgatcatgt ggtcgacgca cgtctcatca agaaaccacc
     8401 cgtgctcgag cgagtggatg tcggcggtga gcgcgttgcg gatgccctcg gcgccttccg
     8461 catgtacggc gacacctttg cgcttcgcgc gggcctctgc cactgcggtg cggatctcat
     8521 cgggagcaag ttgtggctcg tcccagctgt cggccaacga ggtgatcccc ccggtggtgc
     8581 agatcttgat gaagtccgcc cccgccatca gcagcgtgcg caccagcttg cgcatctctt
     8641 cggggccgtc gaccacgccg ctcggaagcc aagcgcgctt ttggaggcgg atgccggcgg
     8701 gcacccagga atcgccgtga ccgccagtct gcgagatcat gacgatcgag atcaagagcc
     8761 gcggtccctt gatgaaccct tccgcaattg cctcgcgaaa tccagcatcc gcgccgccca
     8821 tgtcgcgggc ggtggtcacg ccgcaggcca aagtctcctc aagaatgtgc gtcgagcgca
     8881 gcacgttgta ggtcggtgta ttggtcagat gctggcggat gtcgcgggcc cggtatgtcg
     8941 agtgcacgtg acaatcgatg atgcccggca gcacggtccg tccggcggca tcgagcacgt
     9001 ttgcgccgtg cggaacctgg acggcggtat ccttgcccac ggctgagatg cggccgccct
     9061 caatcacgac cgtgcccttc tcgatgggat cgcggcccag accgtcgatc acgcgtgcgc
     9121 cgatgatcgc tgtggtctgg ttcttaaccg ccatgtttga atcctgatga tgtgaccttc
     9181 tcagcacagc tcgcagacct cctgatcatg gaaagccaaa tgtattgaaa ctcgacccgc
     9241 gcgtgccccg cttgccggga tgacagccgc aaggttatcg attccctagc tcgacgaaca
     9301 cgttcttgac ctcagtataa agggcgagcg catgcatgcc gagctcacgg cccactcctg
     9361 acttcttata gccgccgaag ggggcatagg tgccataccg gctggcactg ccgtgtgagt
     9421 tgatactcac cattccaacg cgcaaagcac gcgcgacacg cagcgcgcgg ccaatgtcgc
     9481 gcgtccagac ggaactgttg agcccgtagt cgctatcgtt tgcaagcccg atcgcttcgt
     9541 cttcgttgtc gaagggaatg acgactacga cagggccaaa aatctcctcg cgggcgatcc
     9601 gcatgcgatt gtcgacctgc gtcataatcg ttggcgcgaa atagcagccg tccggccatt
     9661 gcgccggacg gttgccgcca gagacgatgc aggcgccttc ttccaatcca gcatcgacca
     9721 ggtctttgac gtttcgccac tgggcgttcg ccaccagcgg ccccatctcg gttttcggat
     9781 cgagcggatc gccgacccgg tatttctcgg ttgccgaggc gaaggcggcg agaaacacgt
     9841 catggacttt gcgctcgaca agaagccggc tgcgcgctga acagctctga cccgcattgc
     9901 cgaacccgcc cctgacggct gccaccgcag ccttggcgat atcgccatca gcgaacacga
     9961 tgttcgggct cttgccgccg agctccagcg atacgcgctt gatcccgtcg gcggcggcct
    10021 tgagcagatt cgcgccggtg cgcgtttcgc ccgtgaacgc gatcttgtcg atcaggggat
    10081 tggtggcaat gcgctcgccc aaggccgccc ccggccccgg tagcacattg agaaccccat
    10141 caggcacgcc ggcatcgagg gcgatgcgtc cgagcagcaa ggacgtaagc ggcgtgtagc
    10201 tcgcaggttt caggattacc gtgcagccgg tcaccaatga tggcgctacc ttccacgctg
    10261 ccgccaggaa gggaaagttc cacggggtga tcagcgctac gacaccaact ggctcgcgga
    10321 gcgtgaaatc gagcaccccc tggcccatcg gaatagtctc gccgaaatac ttgtccatgg
    10381 caccagcgta atagtcgaac acgctcgccg ccgcctcaat ctcatttcgg gaatcggtga
    10441 tagtcttgcc accgctgcgc gtctcaacct cggcaatgat atcaacctcg ctgcggatgc
    10501 gatccgcgat ggcgcgcaac atgcgaccac gctcgaacgg cgccagtctc ggccacggtc
    10561 cctcatcaaa tgcgcggcgt gccgccgcgg cagcgcgatc gagctcctcg ggtcccgccc
    10621 ttgcgactcc ggcataggct tggccggtga tcgggctgat caccgtgaac ggattgctcg
    10681 ccgcgcccgc gacgaattgt ccgtcgatta ggagcttggt ctcaagctcg atcaccgctc
    10741 acgctcccga cgaccttcgg acggcaagtt gcaccatgcc gtcaactgcc gcagatcgga
    10801 caacgtgata tcggcctcgg ggggctgctt agaaggctcg gtcgtcaacc atgccgtacg
    10861 cagccccagc gccttgggag ctctgatatc gttgtcgaga ttgtcaccaa cgaacactgt
    10921 ctcctccgca cgcgctccga tgcgagtgag cgccgtgcga tagagctccg gatgcggctt
    10981 gagccaaccc agctcgctcg aaacgacaac cgcgcaaaag tgacggcgca accccagacg
    11041 ttcaagtgtc ccgtagacga ccggcgcgtg cggatagttg gagacgatgg caagccgcac
    11101 atgacgggcc aattcctcag tgacttcgtc agccccgtcc accatttgcg catcttgcgc
    11161 gatcaattct ccaaactggt ccacccatcg cgacagcaca gccatctcgg gcaactgttc
    11221 gatttgcaag agccttaacg ttgtcgcaaa gcggtcctcg aagcggtgct cgcgccgcgt
    11281 cgtcgcagaa tgcgccatgt gcttgcggca ggccttgata taggcggtgc gcagggacgg
    11341 ccacgacacg cccagggcgc gcaggtccgg cccgccgttc tctcccccac actcctcaaa
    11401 caatcgcttg aggcagccga gatgccgctc ggggctgtgg atcatgccgg gaatgcggac
    11461 tagcgtctcg ccccagtcga acagcacggc cttgatggga gtcatcgaat gcgctcgccg
    11521 tcagccgaag cgctgcgctt cccctgcgag tcgtcgggct gcctcggccg gatcgggcgc
    11581 atcatagatc gcgcggccga ctaccgcata gacgttgcaa ccgggcgcgg cggcgaacgc
    11641 atcggcgatg gagccgccga gagtgccgat gccgggaaca aataggcacg ggcgctcgat
    11701 cttctccacc agccagcggg catgatgccg caccttgtcc ggagtattgc ccggcaccac
    11761 gaaatggtcg accccgatct cgatcgcctt gcggaaaatc ttgtccagcg cgtcgtcaat
    11821 cacgtagccg ccgccggttg cattgtaatc ggggaatggg agatcaccgc cgacaatagg
    11881 cagcagccgg ttgaaataag cactgcctgc aaacgctacg accgcacgcg ggcctgcaag
    11941 gggaaagagg atcacgccat cgactttggc ctccttgcag gtcgcagaga atttttgtgc
    12001 catgtcgggt acatcggggc cggccttctg atgatcgtag atcagcggca gatcggtcga
    12061 ggcgcgcaga tggcgaaccg cgccgccgag cccaagccgc agggtcacag tgagtcccat
    12121 tttgtagcca acgacgccgg aaataccgct cgtctgctcg accactttgc gcatctgatc
    12181 gagcgattcc atgtcgaggg caatgaggat tccgtgggtg gcgcgcagcc gcatatcata
    12241 ccatcgtgca atcgccgcct gtgcgagccg gccatcagca agccggcagc tttcgtccag
    12301 ccgcggaaag tcggcaccta cgctcatgac caactccgag cacttccccc ttggtcgcag
    12361 cacgcctgcc gctcgccgat gacccgctcg gtgacgggaa tgcgatggat ggcagtggta
    12421 gtgctccagc tcagcccaag tgcggccgaa cactggctgc gagacacgtg gcgcgtccgc
    12481 tgcgagtgcc atggtccgaa ggagtccagc agtccgtgcg ccaagcattt cacctgttat
    12541 tgaaatgcag accgatgtga agtcaagcag cccgaggacc aggcaacgag tattgcctga
    12601 acggcgcggc atcagaaaac gtgatcggcg ccggcaatta acctagcacc gcgttgagga
    12661 tgctgaccac agcgaccttt gctcgtcccc gaaatgcggc gctagaccat tctcatcaag
    12721 aagagccatc tcatttgtgg cgcacagcgg catgcgacga gacttccccc gcttgcggcc
    12781 gcttgcggga gaggtgaact tgtcactcat gtaccgcgga acttgggagt acgcttgtcg
    12841 tggaaagctt cgacgccttc gcggaaatcg tcggactggc gcagccggct gtagttgagc
    12901 ccttcgagtt cgatggcgat ggagagcgtc gcatcttcgg cgtcgttgag tagcttcttc
    12961 gccgtgcgct gcgccagcgg cgagaaggtc cgcagctcgt cgacaagcgc atcaacggtt
    13021 gcttcgagct tgtcgtcggc tacgcagtcc gttgcgatgc cccactccaa tgcctggcga
    13081 cccgaaattc gcttcgagcg catcacgata tccttcgtgc gtgtgatacc gacgatcttt
    13141 tgcagacgcg ccgatccgcc ggagccgggg atctggccga gcttttgctc gggcaacgcg
    13201 taaaggcagg tctccgagac aatgcggaag tcgcaggcca gcgatatctc gaaaccgacg
    13261 ccgaaggcgt agccgcggtt agcggcgatg accggcttgc tgcagcgcgc aggtgcggca
    13321 atattccagg ccagcttcga gacatgctcg ggagaggctt cgaggaatcc gcgaatgaag
    13381 ccaccggacg agaagtgctc gccgaccgag cgcagcacga tgatgcggac gcgtggatcc
    13441 tcgtcgagag cctcgaaaac ctgccgcaac tggttgcgtt gcggcatcga gatcgtgttg
    13501 aagggcggcc ggtcgagaat gatatcggcg cgttcgcgct cggcagatat ctcgacgcga
    13561 aagccgtcga ggtcctgcag tctcgaatca ggggatgtcg ctggcgaatg catggatcaa
    13621 gccgtctgat ttgaagatat gaaaacggct gtgggcctcg gctccgcttc gtactcgccc
    13681 acaaccagct ttcggcgcag cagcttgccg accggagact tcggaatttc gcggacgaag
    13741 atgtagcggc gcggccgttt gaaattggca aggcccgagg tccggcaatg cgagtcgagc
    13801 tcgtcgtggg caacatctga gcgccgccgg atgaaggcgg ccacgatctt gccccagcgt
    13861 tcgtcgggaa gtccgacgac agccacctct gcgacggcag gatgtagcga gaggcagctc
    13921 tcgatctcta ccggtgagac gttctcgccg ccggtgatga tcatgtcgtc gacccggccg
    13981 gtaacataga tatctccgtc ctcgtccgcg tagccggtat cgcccgtcat gtaccagccc
    14041 tgccggaacg cctgcgcggt ggcttcagga cgctgccaat atccttcaaa ggcttcgtcg
    14101 ccgcacgcga gagcggcaat ctccccttcc tcgcccggcg cggcgatctc atcgacattg
    14161 ctcgcgccaa gcttaacgat ccggatcatc tgattgatgc cggcgcggcc cgccgaaccg
    14221 ggcttgcggg cagcatcctg attgatggtg aaggtgtaaa tctccgtgga cccgtagtga
    14281 ttgacaaaca ggtcgggacg gaaggcgtcg ctcagcttgg caaggaggcc gtccgtcatc
    14341 ggcgcgccgg caaaacccag cttccgcacg gagcttacgt ccgagcgcgc aaaggcctcg
    14401 tgatgcacga tatcgtggta tagggtcggc acgagataga ggttgctgat cctctccttc
    14461 tcaatcagcc gcagcgcttc cacgatgtcg aaacgcggca ggcagacaaa ccggccgccg
    14521 atcagcgaca tggacagcag cgagcgcact cccatcgtgt ggtagagggg catgaccccg
    14581 agcgtgcgtt cgccgtgacc gtaaaggttc tgcgcgacgt gagcgactgc ggctgctcgc
    14641 tcggcgcgat gactccgcgg cacgcccttc ggcttcgccg tagtgccgga tgtataaagc
    14701 atcagcgaga tggcatcggc gtctacgcgc ggcgcggccg gaggtgcatc ggctgcaaca
    14761 agctcggtaa aactggtgtc ggacgacgcg gacttttgcg ccacggaaat gcgcgcgaga
    14821 ctatggcagg cgccgctgtc ggcaacgttc tcggccgaca cctgctcgaa ggcgatcgcc
    14881 ttagcgccgg agttctggac cgcgtaatcg atctcgtcga ccttggcgcg ccagttgatg
    14941 ggcgtgatga cgacgccggc gaactggcaa gcccaatgca aagtggcggc ctcccatcgg
    15001 ttctgcagca cggtgacgag gcgatccccc gccgccagcc gcagctcggc aaagccggca
    15061 accaccgccg atattttggc gtaccaatcc cggtagctca gccgaatgtc gccgtcgaca
    15121 acggcgacgg ctttcggatc gcgggagacg cttgcaagaa agctcgtccc aagatcaagc
    15181 atggagggac tcccggcgca gtgtggcggc agcttcgagc gccgccgcga gaatgggtgc
    15241 atagccggtg cagcggcaga ggtggccacc aatgacctct ttcaactcct cctccgatgg
    15301 ccgaagtgat gtcgataagt aggcgtgcag cgacatgagg atacctgcgg tacagaaacc
    15361 acactgcaga gcgtgatggc ggcggaaggc tgcttgcagc accgagagcc ggtcgggaga
    15421 cggtgcgagt ccctcgacgg tgcggatgtc gcaaccttgc gcctggactg cgagcatcaa
    15481 gcaggaacgg gccggcaggc cgtcgacgat gattgtgcag gctccgcaca cgccatgctc
    15541 gcaacccaca tgcgtgccgg tcgcgcccac gatatgacgc agtgcatcgc agagcagcat
    15601 tctggactcg acttccatgg aagtcgcctt gccgttgagg gtgaagctaa cctgatggcg
    15661 gcgatcgggg ttcagcttcg gcatgcagcg gcctctaaca atacttttcg accgagcttg
    15721 cgcacgagct cgcgccgata gcgcgccgtg gcgtgaatgt cgtcgctgcc acggagctcc
    15781 caggcgaagg cgttgagcgc atcgtcaagc gcggttcctt cgaggagtgg aaaatcccgt
    15841 gcggtcggcc tgtcagcgac gcctgcgatc gcgagccgca tgcgctccgc atcgaccaca
    15901 gccgcgcagg cgacgatggc aaagtcgccg tgccgccgcc ccacctcgat gaaggcatag
    15961 ccagctccgg ggcgacgctt cggataggca accgcctcga caagctcatc atcttcccga
    16021 tcggtcgcca tcatgccggt aaagaatgct tcggcagtga cgcggcggcg cttgcgtcgc
    16081 gcaagcagcc gcacctcgcc ctccaacgcg acgagacaga gcgggatctc ggcgctcgga
    16141 tccgcatgtg cgaccgagcc gcagattgtt ccgcgtgacc gagtctgcag gtgtccgacc
    16201 cacggcagag ccgctgcaag cagcggaacg tcacgcttca gggcgggatg cgccattgct
    16261 gccgattgcc gtaccgccgc tggaatggtg atggcatcgg tctcctcgat gatttgcgcc
    16321 agcggctcga cgcgcatgag gtcaaccagc aaggcgggcc tggcaagccg catgttcagc
    16381 atggcgatga gtgactggcc accggcgagt acgcgggctt cgctgccgtg ctcctgcagg
    16441 gtggcggtga tttcgtccac cgtccgggcg cagatgtaat cgaacgcggg cggcttcatc
    16501 ggcgcaatcc aatcaaagct agaagccgcg cggtccagcc cgggcgcgcg ccgccggcct
    16561 tgcgtgccag cgcggcgaag aactgtttga agatgacccg cgccgcgcca tccagaagcc
    16621 ggccaccgac cgcagcgacc ttgccgccga gggcggcctg ataggtatag ccgatgatcg
    16681 tgccaccatc gcagtccggc gtgagggtca ctcgaccatt gccaccgccg ctgccgagcg
    16741 caccgaccac ggtacccgtc agggtcgcag accacggcgg gtcgaggtcc gacagacaca
    16801 cctccgcctt atagcggcct ttcactggcc cgacgcccaa agtgacgtct gccacgaagt
    16861 gagtgtctga gaatttcttc accccatagg aacccgggat cagtgaggcg agcacatccg
    16921 catcgagcag catcgcccac accgccgccg gggcggcctg aacccgcacc tctccggccc
    16981 cggtcatggt gcggtcgccc ggcttggcaa ccctgagcgg agccgtcatt cctttgggtg
    17041 gcaacggctc gtcgcccgaa acgctcgctg caagcttggc cggcgtcagc gggagatcga
    17101 tggttgcgag gttaagcgcg tcggcgactg cgttggcgag gcaagcgggt gtcgacatgc
    17161 agttgccctc gcccactccc ttggaaccca agggcgtgaa cggggatttg ctttcgatat
    17221 gcaggaccgt gaggtccggc acttctgtta ccgtcggaag cagataatcc gcaaacgtgc
    17281 cagagaggaa cgagccgtca tcgccatacg acagctcttc gtagaatgcg gcacccacgg
    17341 cctgcgcgaa accaccgcga atctgccctt cgaccatgcc gggatgcagg atgcgcccgc
    17401 agtcgtgggt cgtgacatag cggtcgatgc gtaccttgcc ggtgatgcgg tcaacctcga
    17461 caccacaaaa atcgaagatg aagccatggc agagcgacga gttgatctcg tcggcgtcgg
    17521 tcggtgctgt cagctgcggc ggcgtccaga acaccgtctc ccggattgtg tcgctcatct
    17581 catcgggaag cagtcccggc gcccaatggc ttatcgccgc aaggcgtgaa aaagaaatcg
    17641 ccgcatccgg gttggtcttg gcaacgacct tgccgccggc aaatacaagg tcttcgggcc
    17701 gcacattgag ccgtgcggcg gcgatccggg aaagcttgtc gcgaagcctg ctcgcggcca
    17761 gatgcgtcgc tcccgccact gccggagcga agcggctcga ataatttccg gatgcgatgg
    17821 accatgcatc cttgccggta tcgagatcag tggtcacgcg cacttccgtc atcgacaggc
    17881 cgaaaacgtc agcgacaact tgcgaaagca ccgtgcgatg accttgtcct tgcggcgttg
    17941 aggcgacctg aacggttacc gagccgagcg ggtcaagggt aatgctcgcc gttgcctgcg
    18001 cgccgtcctt tgcgcccgcc ttgcgacgtt cggcggccgt gagcgccgtg gtgatgtaac
    18061 ccatattcga cacgctgggt tcgacagcgg cggtgtagcc aatgccatag aggcggccag
    18121 cagcgcgtgc ttcgtcgcgc cgacgcctca gctcatccag cgcgccatcc cttgcggcga
    18181 cttcgagcgc gcgggcgtaa tcgccggaat cgtagatagc tccggtcgcg gtgtgatagg
    18241 ggaaggccga tgccgggata aggttgcggc ggatcacgtc tagcggatcg agctgcaatt
    18301 cgacggcgat ccgctgcatg aggcgctcca gcggatagta gacctgcggt cctccgaagc
    18361 cgcgtaccag tcccgtaggc gctttgttcg tgactacaac ccggttgcgc agacgcacat
    18421 tgcggatgtc ataggcgccg gtcatattgc cgtgcatccg atagagggtg gccggctccg
    18481 gggggcgcag ataggcgccg caatcctcca actgatccca gtcaagcgcg ctaatatgtc
    18541 cgtccgcgtc gacagccgct ttcaaggtgc tgacgcggtt cgtcgctgac accgaggcgg
    18601 caagatgctc caggcgatcc tcgacccatt tgacaggccg tccagccatg cgtgccgccg
    18661 ccgcaacaag aaccgcatat ggcgcaattc cctgcttgac gccgaagctg ccacccgagt
    18721 cctgcggcgt gcgcaggcgc agccggtttc ccggcacctt gagcgcacgc gcgatcaccg
    18781 catgcaggct gaagggaccc tgaaaattag ccaggatctc ataggcatcc ccgtgcggat
    18841 cgtagtgcgc gacgacacca taagtctcga tgggcgagcc ggtattgcgc ggataatgcg
    18901 ctgtcaccga aacgcagcgg gcagccgcgg cgaaagcagc gtcgggatcg ccgtaaacga
    18961 atgtgcgatc gctgaccaga ttgctgccca ccgcctcgtg tagaatcggc gcctcggcat
    19021 ccagtgcgtt ctgcggatcg acaaccacgg gatgctgccg gtattccacc tcgatggcgt
    19081 cgatggcatc ctcggcccga tagcggtcgc tggccagtgc gatggcaacc ggttcgccga
    19141 catagcgcac gcgctcgacc gcgatcgcac gagcctcgat aggcgcacgc acgcctgaaa
    19201 tgagcggcgc ggtaatggcc gcgagatcca gtccgctcat gaccgcgaag actccgggca
    19261 gcgcccgcgc cggttcggtt cttatcgata cgatatcggc atgtgcatgt gggctccgca
    19321 gaattgccgc atgcagagtc ccgggcttga tgccgagatc gtccatatag gcgccgcggc
    19381 cactcagcag cgcggcatcc tcaatgcgct ccagcgactg cccaacaagg gctgctcgat
    19441 ccattgtcaa ctgcatgatg acgtcttatc gcggactcct ccgcctgttg gatgtctttc
    19501 aactggatct cggcgttcgg ggcgttgtcg acctcgccaa acggctcgtc ggagggggca
    19561 gcggcgcagg tcatgagtgc ccacgattgg ccatcaaacc atcggacgat tgccgccttt
    19621 caaccagact catgcactgc agcaaacgac gtgctcacaa atctactata tcaacctccg
    19681 atcagcggga tctttccgtc ggtgatcact ttcttccatt tggcgatgtc ggcgttcaca
    19741 taggccgcga aatcggccgt ggaattgcct tccgcctcga cgccggcctg cagcagcttt
    19801 gccttgatgt cgggcgcgtt cacgatcgcc gagatttcgc gctgcagcag gtggacgatc
    19861 gccttcggcg ttcccgccgg cacgaacacg ccagtcatgg tttccgcctc ctgcccagca
    19921 atgccgatgt cttcgagagt cggcacatcg ggcaacgccg ccgaacgctt cttggcggtg
    19981 actgcaagcg cgcgcagctt gccctccttg atcaggttga cgtaattgcc gagggcagcg
    20041 cagacgatcg gcgtgtgccc accgaccagc gattgcgtca tggggccacc gccctgaaat
    20101 ggcacggtga cgaaatcgag gccaagggcg agcttaagca tttcgatcga cagtgacggt
    20161 gtcgtgccgg taccgggcga tccgacgct
//
LOCUS       DATLJT010001210        12269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2619202,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001210 DATLJT010000000
VERSION     DATLJT010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12269
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619202"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VM910_27895"
     CDS             complement(<1..516)
                     /locus_tag="VM910_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HVI66372.1"
                     /translation="MEAIFRVDGDRVEVSPNAAGPWDPRMQHGSAPAALAVWAAEQIP
                     TAVDMRIARVTIDLMRPVPLKPLTLKSEVLREGRKIQLCAISLSADGVVAVNARVLKI
                     KKQILALPSDIADLPVELPPPEQSRQDPANFSSSAFVTGMSVRAARGHFGMKGPGAIW
                     YRVERPLIEGFG"
     gene            complement(565..858)
                     /gene="minE"
                     /locus_tag="VM910_27900"
     CDS             complement(565..858)
                     /gene="minE"
                     /locus_tag="VM910_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HVI66373.1"
                     /translation="MSILRFFSSRAARPAVATAPIARDRLQILLAHERAGMRGQPDLL
                     MQLREEILAVVSRHVALDPEKVMVRMERGKNVSTLEVDIELPNESGVSLAATG"
     gene            complement(855..1670)
                     /gene="minD"
                     /locus_tag="VM910_27905"
     CDS             complement(855..1670)
                     /gene="minD"
                     /locus_tag="VM910_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HVI66374.1"
                     /translation="MAKVLVVTSGKGGVGKTTSTAAIGAALAQAGKKTVVIDFDIGLR
                     NLDLVMGAERRVVFDLINVVQGVAKLPQALIRDKRLEKLWLLPASQTRDKEALTEEGV
                     GRVIADLRTSFDWIVCDSPAGIERGAMLAMRFADAAIIVTNPEVSSVRDSDRIIGMLD
                     AKTVKAEAGERVEKHVLITRYDVSRAARGEMISIDDILEILATPLLGIIPESQDVLRA
                     SNVGSPVTLNNPASAPARAYHDAMRRLLGETVPMQVPSERKGLMDLLRGRRAA"
     gene            complement(1700..2392)
                     /gene="minC"
                     /locus_tag="VM910_27910"
     CDS             complement(1700..2392)
                     /gene="minC"
                     /locus_tag="VM910_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HVI66375.1"
                     /translation="MDASAEPTPQPVRLRGRSYVAFVFAPAVPIIDWLAEIDATVARS
                     PGFFVGKPIVLDLSAVELSRAAIAHLLVSLEKKNIRVLGIEGVDATLLTPTMPPLLTG
                     GRHCAVREAVPEKPEVKPPPTSLLLDSPVRSGQSVVFPDGDVTILGSVGSGAEIVAGG
                     SIHVYGTLRGRAMAGVNGNSAARIYCQKIEAELLAIDGYYQTAEELDAALRNRPAQAW
                     LEGSMMRVTPLN"
     gene            2877..3476
                     /locus_tag="VM910_27915"
     CDS             2877..3476
                     /locus_tag="VM910_27915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66376.1"
                     /translation="MSVPSEQPREQAPSTNPQDPLHYAPRRFSERTESRLAAVVGDIQ
                     AADRASRPDPAVRSIAPSTAMNAKLENAVYESLLRQIDPQVVPEPADFKRERSQRALI
                     GVVIGVAAAIVVSAAVALAYVTMFPGAKDKVPGASLATTAAQPRQTEDRSKPVPALAQ
                     FRGLLVAGESGQAFTHEQSEQLLQQFMQWRQKADSPEKP"
     gene            3617..5260
                     /locus_tag="VM910_27920"
     CDS             3617..5260
                     /locus_tag="VM910_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66377.1"
                     /translation="MSAAPGDNVSVVRELASRVGPIVGSALACQDIAQSRIQVIADKF
                     RAVIREVSSNEAERDDLSRMFDRFANDGRGAVTTGRTDCKTAARQLAELEQSLAGASL
                     AGVIGPSAAVAAPAPAPAAPTAPIAPAASTALAVPAAPPVPPAPPAPPAAAPPATMAT
                     ATPTITQAVPLPASNVRGITSNEIRFGIVAPFSGSARELGRQMKLGIDTAFNRINDAG
                     GVDGRMLRLVAADDGYEPSRTLDGMKQLYDKDQVFGYIGNVGTPTAQVAIPYALEHRA
                     LFFGAFTGANLLRNDPPDRYVFNYRASYAEETDAVVHYLIKIRRLQPRQIAVFAQQDA
                     YGDAGYAGVAKAFRSLGVNDAAILRLGYKRNTVDVDDAVSQLKGQKLAIRAVVMVATY
                     RAAAKFIEKTKDQYPGMIYTNVSFVGSTALADELMLLGPRYASGVIVTQVVPAVGGYS
                     SAVLEYKNALQKYFPGEAPDYVSLEGYIAANVLIQALKKTGPQVDTEKLIDTLEAMRN
                     MDLGLGAPLNFGRAEHQASHKIWGTAIDENGKYTPIELE"
     gene            complement(5422..6390)
                     /locus_tag="VM910_27925"
     CDS             complement(5422..6390)
                     /locus_tag="VM910_27925"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HVI66378.1"
                     /translation="MSMPALFKGRLSLPVIGAPLFIISVPDLVIAQCKAGVVGSFPAL
                     NARPPTLLDEWLARIKEELAAYDKAHPERPSAPFAVNQIVHKSNNRLDHDLALCEKNR
                     VPMIISSLGAREELNRAVHNWGGIVFHDVINQKFAHKAIEKGADGLILVAAGAGGHAG
                     VISPLAFVAETRQWFDGPIALSGAIANGRAIRAARILGADFAYIGSAFIATREANAVE
                     GYKQMITNAAAEDIVYSNLFTGVHGNYLKPSIVAAGMDPDNLPTSDPTKMSFGTDASG
                     ERAKPKAWKEIWGSGQGVGSVGKIVPAAELIARFKKEYDDALDPPL"
     gene            complement(6483..7010)
                     /locus_tag="VM910_27930"
     CDS             complement(6483..7010)
                     /locus_tag="VM910_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66379.1"
                     /translation="MPILAVIAAGVALAQTAPPQPSLGLREQPAQQGTPPPPNPPAAQ
                     QSPSPSKNPGLINEMGKLFEKSLSILPPLKTPGETIDDLNARAKDAGEALSRLAKPGS
                     MVSGRVICPVGANGTPDCKSGADKLCQSKGYKEGKSLNTDSAQTCSAKVMIPGRQRKP
                     EDCRTDNYVTTALCQ"
     gene            complement(7113..7694)
                     /locus_tag="VM910_27935"
     CDS             complement(7113..7694)
                     /locus_tag="VM910_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HVI66380.1"
                     /translation="MLRRIYDWCVDAADKPYAVWILGGVAFAESSFFPVPPDIMLLPM
                     SLAQPKRAWLYASVCTVASVLGGVLGYAIGALLYDSVGHWLINLYGLGDKVEAFRASY
                     AEWGALIILLKGLTPIPYKLVTITSGFAGYNIWLFILFSIITRGGRFFMAAIVLNRYG
                     EWIKVRIERHLALSVAILAIVLVAGFIVAFRLI"
     gene            complement(7815..8216)
                     /gene="hisE"
                     /locus_tag="VM910_27940"
     CDS             complement(7815..8216)
                     /gene="hisE"
                     /locus_tag="VM910_27940"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HVI66381.1"
                     /translation="MGDSIERLYQAVLAARDLDPATSRTARLFQRGPAKMAKKLAEEA
                     IEVVIDSVSGDTQAVVRESADLLYNLTVLWASAGVRPEDIWLEMERRELLLGIAEKMP
                     KSAVKLAKTVAQRPGRRPIVALDRNRLRKRH"
     gene            8523..9578
                     /locus_tag="VM910_27945"
     CDS             8523..9578
                     /locus_tag="VM910_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66382.1"
                     /translation="MRQFRMNAFRCALTLLAGLAVLAFVRVEKVRAGETRQPLAIELS
                     FDRPLEASMAPFVVAERRGLFAAERLSVANNFADGSVDAIARVASGASEFALVDLNEL
                     IRYRDRADAQPIKAVFVLFNKAPYAIVARKSRGIRALSDVVGKTLGVAESDLSFRLWP
                     ALARQNGIRPESVKVYRMSAAVREPILSAGQVDAVAGFSYLSAVNLRDRGVPANDLTV
                     LRYADYGCEAYGFAIVVNPTFASAKPDAVKGFVRAVIAGTHLAIKDPQRAVDDVVSLM
                     DGASRELELERLRTVITDNILTGEVKRHGLGGIDPARLERSIGQIAEDYKFRKRPTAD
                     DVFDGGFLPPVGGRLIN"
     gene            9655..11424
                     /locus_tag="VM910_27950"
     CDS             9655..11424
                     /locus_tag="VM910_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HVI66383.1"
                     /translation="MSLVRLYIRVLELLGKEAKLGWILAGANLLLAEAQFAEPVLFGR
                     IVDVLSGRMAPGTSAWPLLAAWAAFGLFTIVAGAAVALYADRLAHRQRQAVLSSYFEH
                     ILQLPLTFHTGTHSGKLMKVMLNGTDALWRLWLGFFREHFAAIMSVVVLLPLSLYINW
                     RLAILLFALCVVFTVLTTLVVRKTYGMQNEVEEHYSDLSARASDALGNVALVQSFVRI
                     DAEVQGLRFVADKLLAAQMPVLSWWALVTVITRASTTITVLAIFTVGIALHQAGLTTV
                     GEIVMFVSFATMLIQKLEQVVSFINNVFMEAPRLREFFNVLDAVPAVRDRPDAIEVGR
                     LSGLVDFNDVSFSYDGKRPAVEDLSFTALPGETIALVGPTGAGKSTAIALLHRAFDPQ
                     SGFIRIDGMDVRGLKLTALRRNIGVVFQEAMLFDRSIADNLRIGKPDATDEELRIAAA
                     RAQALDFIERNERGFDTKAGERGRMLSGGERQRLSIARALLKDPPILILDEATSALDA
                     VTEAKVNAALDEVMKGRTTFVIAHRLSTIRNATRILVFDNGRVIESGTFDELVAKGGR
                     FAALARAQFMVQGDARANNAVKA"
     gene            11599..>12269
                     /locus_tag="VM910_27955"
     CDS             11599..>12269
                     /locus_tag="VM910_27955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVI66384.1"
                     /translation="MTGIGIVQLFHQLLSRSLLRGIALLAVLAIFAPRALHAEARLVV
                     EADSGKVLEAENATVPWYPASVTKLMTAYVTLRAVKDGRVTLDTLFTVSPVAASQSPS
                     KMGFRPGTQVTVDNALKMMLVKSANDMAVVLAEGVGGSIDGFSVMMNQTAQRLGMTQS
                     HYVNPNGLPADEQVTSARDLAILARAIIRDLPEYEYFVHIPSIRLGHRITQNFNRLIG
                     RYPGA"
BASE COUNT         2244 a         3963 c         3822 g         2240 t
ORIGIN      
        1 gccgaaacct tcgatcagcg gccgctcgac gcggtaccag atcgcgcccg gacctttcat
       61 gccgaaatgg ccgcgcgccg cgcgcaccga catgcccgtg acgaaggcgc tcgaggaaaa
      121 attggcgggg tcctgacgcg attgctcggg cggcggcagt tcgaccggaa gatcggcgat
      181 gtcggaaggc agcgcaagga tctgcttttt gatcttgagc accctcgcat tcacggccac
      241 gacgccgtcg gcggacagac tgatcgcgca aagctggatc ttgcggccct cgcgcagcac
      301 ctcgctcttg agcgtcaacg gcttgagcgg caccggacgc atcaaatcga tggtgacgcg
      361 cgcaatgcgc atatcgaccg ccgtcgggat ttgctccgcc gcccacacgg ccaaggccgc
      421 cggcgccgag ccgtgctgca tgcgcgggtc ccacggacct gcggcgttgg ggctaacctc
      481 gacgcgatcg ccgtcgacgc gaaaaatggc ttccatcaat cttgcggccg cgggacgagc
      541 ccgtttgtga cactcagccg acggtcagcc cgtcgcggcg agcgaaacgc cggactcgtt
      601 gggaagctcg atgtcaacct caagcgtcga gacattcttt cctcgctcca tccggaccat
      661 aaccttttcc ggatcgagcg caacatgacg cgacaccacg gcgaggattt cctctcgcaa
      721 ttgcatcagg aggtcgggtt ggccgcgcat gccggcacgt tcatgcgcaa gcaggatctg
      781 cagccggtcc cgcgcgatcg gagccgtggc gaccgccggc cgcgcggcgc ggctggaaaa
      841 aaaacgcagg atgctcatgc ggccctccgt ccccgcaaca ggtccatcag acccttgcgc
      901 tcggacggca cctgcatggg cacggtttcg ccgagcagcc gccgcatcgc gtcgtgatag
      961 gcgcgtgccg gcgcgcttgc cggattgttc agcgtgaccg gcgagccaac atttgaggcg
     1021 cgcagcacat cctggctctc cgggatgatg ccgagcagag gcgtcgccag gatttccaga
     1081 atgtcgtcga tcgagatcat ctcgccgcgc gcagcgcgcg aaacatcata gcgcgtgatc
     1141 aggacgtgct tttccacgcg ctcgcccgcc tcggccttca cggttttggc gtcgagcatg
     1201 ccgatgatgc gatcggaatc gcgcaccgac gagacttccg gattggtgac gatgatcgcc
     1261 gcgtcggcaa agcgcatcgc gagcatcgcg ccccgctcga tgccggccgg gctgtcgcag
     1321 acgatccagt cgaagcttgt ccgcagatcc gcgatgacgc gcccgacgcc ctcttccgtc
     1381 aacgcctcct tgtcgcgggt ctgcgaggcc ggcaacagcc agagtttctc caggcgcttg
     1441 tcgcggatca gggcctgtgg aagcttcgcc acgccctgca ccacgttgat gagatcgaac
     1501 acgacgcgcc gctcggcgcc catgacgaga tcgagattgc gcaggccgat gtcgaaatcg
     1561 atcacgacgg tcttcttgcc cgcttgcgcg agcgccgctc cgatcgcggc ggtcgaggtg
     1621 gtcttgccca cgcctccctt gcccgacgtc acaaccagca ccttggccat ttcttacctc
     1681 cttttactat gggttcaatt caattgagcg gcgtaacccg catcatgctg ccttcaagcc
     1741 acgcctgcgc tggtcgattg cgaagagcgg cgtccaactc ttccgcggtc tggtaataac
     1801 catcgatcgc cagaagctcg gcttcgatct tctggcaata gatccgcgcg gccgaattgc
     1861 cgttgacgcc cgccatggcg cggccacgga gcgtcccgta aacgtgaatc gagccgcctg
     1921 cgacgatctc ggcgccggag ccgaccgatc ccaggatcgt gacgtcgccg tcgggaaaca
     1981 ccaccgactg tccggagcgg acggggctgt cgagcaggag cgaggtcggc ggcggcttga
     2041 cctcgggttt ttccggcacg gcctcgcgca cggcgcaatg gcgcccgccg gtcagcagcg
     2101 gaggcatggt cggcgtaagc agcgtcgcat ccacaccttc gatcccgagc acgcggatat
     2161 tcttcttctc aaggctgacg agaagatgcg cgatcgcggc gcggctcaat tcgacggcgg
     2221 aaagatcgag cacgatcggc ttgccgacga agaagcctgg cgagcgggca accgtggcgt
     2281 cgatttcggc aagccaatcg atgatcggca cggccggcgc gaacacaaaa gcgacatagg
     2341 agcggccgcg cagacggaca ggttggggtg tgggctctgc acttgcatcc atggctgacg
     2401 actcgggttt cttatcgaat ggttaaccat cgacacgcca tggttaatgg tcagttaatg
     2461 cccgtcattt ctgctctttc ggcctgttcc cgccccacga atgacaaaga aggggtgtcc
     2521 gctgtcaaaa actcaacgcg gttcggcctt gatttgagct taataatgac gcgccccggt
     2581 tgaatcactt ctcggtgaga caagcagact gtctttttcc gcttggctgg cggcgtttgc
     2641 ttggtacggg aagaggggat cgaggggcac gccgtggtct cgggatgttt cttgcgaccg
     2701 agggtcacgc agccgcagaa ggcggcggtc cgaagtcttt tgattcaaat cgcgcagaag
     2761 atttttgcgt ctcccgcacg gcctgcgcgt attttggcga gcactctttg ctttgaacct
     2821 ttgttcgatt tggtcgaaaa aagctttcat aaagtaagtc gtcagaggag tagcgtatga
     2881 gtgtcccgag tgagcagccc cgcgagcagg cccccagcac gaatccgcag gatccgctgc
     2941 actacgcgcc gcgccggttc agcgaacgaa cggagtcgcg gctcgccgct gtcgtcggcg
     3001 acatccaggc agcggatcgc gcctccaggc cggacccggc cgtgcgcagc atcgcgccat
     3061 cgaccgcgat gaacgcaaaa ctcgaaaatg cggtttatga atccctgctg cgccagatcg
     3121 atccgcaggt ggttcccgag ccggccgact tcaagcgcga gcgctcgcag cgcgcgttga
     3181 tcggcgtcgt catcggcgtc gccgcagcca tcgtggtctc ggctgcggtc gcactcgcct
     3241 atgtcaccat gttcccgggg gctaaggaca aggttcccgg cgcctcgctg gcgacgacgg
     3301 cggcgcaacc gcgccagacc gaagaccggt cgaagccggt gccggcgctt gcccaattcc
     3361 gggggcttct ggtcgccggc gaaagcggcc aggccttcac ccacgagcag tcagaacaac
     3421 tcctgcagca attcatgcaa tggcgccaga aggccgattc acccgagaaa ccctgaccaa
     3481 agacacagta ttcaagcgcg gagaaaccaa catgcctgat cacggcacca acatgattct
     3541 gacgcgccgg ttgcgctttt caacacgcgc ggcgaccgcg ctccttggcg cttggctcgc
     3601 cggtactgcg ggcgcgatgt cggcagcgcc cggcgacaac gtctccgtgg tgcgcgagct
     3661 cgcgagccgg gtcggaccga tcgtcggctc ggcgctggcc tgccaggata tcgcgcagtc
     3721 ccgcatccag gtgatcgccg acaaattccg tgccgtcatc agggaagttt cctccaacga
     3781 agccgagcgt gacgatctct cacggatgtt cgaccgtttc gcaaatgacg gccgaggcgc
     3841 ggttacgaca ggcaggacag attgcaagac ggcagcccgt cagctcgccg aacttgaaca
     3901 atcgctggcc ggcgcctcgc tggcaggcgt gatcggaccg tcggctgcag tggctgcccc
     3961 ggcgccggcg cccgccgcgc ccaccgcacc gattgccccg gccgcatcga ctgcgctggc
     4021 cgtaccggca gcacccccgg tacccccggc tcctcctgcg ccgcccgcgg ccgcgccgcc
     4081 cgccacgatg gcgacggcga ccccgactat cacgcaggca gtgccgcttc ccgccagcaa
     4141 cgtccgcggc atcaccagca acgaaatccg cttcggcatt gtggcgccct tctccggctc
     4201 ggcgcgggaa ctcggtcgcc agatgaagct cggcatcgac accgcgttca accgcatcaa
     4261 cgacgccggc ggcgtcgacg gcaggatgct gcgtctcgtc gcggccgacg acggctacga
     4321 gccgtcgcgc acgctggatg ggatgaagca gctctacgac aaggaccagg tgttcggcta
     4381 tatcggcaat gtcggcacac cgacggcgca ggtggcgatc ccctacgcgc tcgagcatcg
     4441 cgcgctgttt ttcggcgcct tcaccggcgc caacctcctg cgcaacgatc cgcccgaccg
     4501 ctacgtgttc aactatcgcg caagctatgc cgaggagacc gacgcggtcg tgcactatct
     4561 gatcaagatc cgccggctgc agccgcggca gatcgccgtg ttcgcccagc aggacgccta
     4621 cggcgatgcc ggctatgccg gcgtggccaa ggcgttccgc tcgctcggcg tcaatgacgc
     4681 cgcgatcctc aggctcggct acaagcgcaa caccgtcgat gtcgacgatg cggtcagtca
     4741 gctcaaggga cagaagctcg cgatccgcgc cgtggtcatg gttgcgacct atcgcgccgc
     4801 ggccaagttc atcgagaaga ccaaggacca gtacccgggc atgatctata ccaacgtctc
     4861 gttcgtgggc tcgaccgcgc tcgcggacga attgatgctg ctcgggccgc gctacgcgtc
     4921 cggcgtgatc gtgacacagg tggtgccggc ggtcggcggc tattcgagcg cggtgctcga
     4981 atacaagaac gccctgcaga aatattttcc gggcgaagcg cccgactatg tttcgctcga
     5041 gggctatatc gccgccaatg tgctgatcca ggccctcaag aaaacgggcc cgcaggtcga
     5101 caccgaaaag ctgatcgata ccctggaggc gatgcgcaac atggatctcg gcctcggcgc
     5161 gccgctcaat ttcggtcgcg cggagcacca ggcctcgcac aagatctggg gtaccgcgat
     5221 cgacgagaac ggcaagtaca cgccgatcga gctggagtag gcggtcctca ccttcgctga
     5281 ggttcagcga acaaacctcg gttctaacct cccctcgaaa aggggaggtt agtttgctcc
     5341 gtcagaagca aattgatccc tttcccggag ccatcgcgct ttatgatcga ttgaagcttt
     5401 gcaaagtgtc gaatccgctc ctcacagcgg cggatccaac gcgtcgtcgt attccttttt
     5461 gaagcgcgca atcagttcgg ctgccggcac gatcttgccg acactgccga cgccctggcc
     5521 cgaaccccag atttccttcc aggctttcgg cttggcgcgc tcaccggagg cgtcggtgcc
     5581 gaaactcatc ttggtgggat cggaagtcgg cagattgtcg ggatccatgc cggcggcgac
     5641 gatcgacggc ttcaggtaat tgccgtgaac gcccgtgaag aggttggaat agacgatgtc
     5701 ctcggcggcc gcgttcgtga tcatctgctt atagccttcg actgcattgg cctctctggt
     5761 cgcgatgaag gccgagccga tataggcgaa atcggcgccc aggatgcgcg ccgcgcgaat
     5821 ggcgcggcca ttggcgatcg cgccggacag cgcgatcggc ccgtcgaacc attgccgcgt
     5881 ctcggccacg aaggcgagcg gcgagatcac gccggcatga ccgccggcac cggcggcaac
     5941 caggatcaac ccgtcggcgc ccttctcgat cgccttgtgc gcgaatttct ggttgatcac
     6001 gtcgtgaaag acgatgccgc cccaattgtg cacggcccgg ttcaactctt cgcgcgcgcc
     6061 cagcgacgag atgatcatcg gcaccctgtt cttctcgcaa agcgcgagat catgatcgag
     6121 ccggttgttc gacttgtgca cgatctggtt gaccgcgaac ggcgccgagg ggcgttccgg
     6181 atgcgccttg tcgtaggcgg caagctcctc ctttatgcgc gccagccact cgtcaagcag
     6241 cgtgggcgga cgcgcgttca gcgccggaaa cgaaccaacc acgcccgcct tgcattgcgc
     6301 gatgacgaga tcgggcacgg agatgatgaa tagcggcgcc ccgatcacgg ggagtgacag
     6361 gcggcctttg aacaaggcag gcatcgacat gttgcaaaaa ttcctttttc cggccggcgg
     6421 gaaacgcttt ccgctcaaag atgccagaca tcatcccacg aaagcaacgc gcaaaatcgc
     6481 gcctactggc acagcgcggt cgtgacgtaa ttgtcggtgc ggcagtcctc gggcttgcgc
     6541 tgccgtcccg gaatcatcac cttggccgaa caggtttggg cggaatcggt gttgaggctt
     6601 ttgccctcct tgtagccctt gctctggcag agcttgtcgg cacccgactt gcaatccggc
     6661 gtgccgttgg ccccgaccgg acaaatcacg cgcccggata ccatcgagcc cggcttggca
     6721 agccgcgaca gcgcctcgcc cgcatccttg gcgcgcgcgt tgaggtcatc gatggtttcg
     6781 cccggagttt tcagcggcgg caaaatcgac agcgatttct cgaacagctt gcccatttcg
     6841 ttgatgaggc ccggattttt cgacggcgaa ggtgactgct gtgccgccgg cgggtttggc
     6901 ggcggcggag ttccctgctg cgccggctgc tcgcgaagac cgagcgaggg ctgcggcggc
     6961 gcggtctgcg caagagccac tcctgcggcg atcacggcca ggatcggcat caccgcagcc
     7021 gcagcgaccg gcaccaggca tcgcgacgcc aggcgagcgg agcggatttg acgttcgtta
     7081 tccaatttgc cccgccgaac cgtcatgcct tgtcaaatca aacgaaacgc aacgatgaaa
     7141 cccgccacca gcacaatggc gaggatagca accgagagcg ccaagtgccg ttcgatcctg
     7201 accttgatcc attcgccgta gcgattgagc acgatcgcgg ccatgaagaa acgtccgccg
     7261 cgggtgatga tggagaacag gatgaacagc cagatattgt agccggcgaa gcccgaggtg
     7321 atcgtgacga gcttgtaggg gatcggcgtc aagcccttca gcagaatgat gagggcgccc
     7381 cactcggcat aggaagcgcg gaacgcctcg accttgtcgc cgaggccgta gagattgatc
     7441 agccagtgtc cgacggagtc atagagcagc gcgccgatgg catagccgag cacgccgcca
     7501 agcacggaag ccaccgtgca gaccgaggca tagagccagg cgcgcttcgg ctgcgccagc
     7561 gacatcggca gcagcatgat gtcgggagga accggaaaga acgaactctc cgcgaaggcc
     7621 acacccccca agatccacac cgcatagggc ttgtcggcgg cgtcgacgca ccagtcataa
     7681 atccgtctga gcatgggcgg gatgaaccat catggacggc atttgtccat gcccgcaggc
     7741 ccctgaaacc agcttgaact atctcggaga acccgctccc tccccccggc aggtcgaagg
     7801 cagcgaaaaa tggtctaatg ccgcttgcgc agacggttgc ggtccagcgc gacaattggt
     7861 cgccggcccg ggcgctgtgc caccgttttg gcgagtttca ctgccgattt cggcatcttc
     7921 tcggcgatcc cgagcaggag ttcgcgccgc tccatctcca gccagatgtc ctcgggccgc
     7981 acacccgcgg aggcccaaag cacagtgagg ttatagagca gatcggcgct ttcgcgcacc
     8041 acggcctgag tgtcgccgct gaccgagtcg atcacgacct cgatcgcctc ttcggcgagc
     8101 tttttcgcca tcttggccgg cccgcgctga aacagccgcg cggttcgcga cgtcgcggga
     8161 tcgagatccc tcgccgcgag caccgcctgg tacagccgct cgatcgaatc acccatgaca
     8221 cgggaggata aagcaaaccc gtgcccggcg tgttaacgcg cggcacaaaa agcaggatat
     8281 ccgccggtca tgccttcatg aacggtggat tacgctgata gctattcacc agctcgcgag
     8341 cggatatggt tgacgttggg ttcccgaggg ctcgttgcag gccgaaacgg cgctatctgg
     8401 cgctcatgga ccgttcatct ttgctgggcc acaataaaac tgctggcgca gactgccata
     8461 tttcagcctt cccgaaaaac cccaatggcg ccagcctgga tcgcttgaaa ccggcgagtt
     8521 cgatgcgtca gttcaggatg aacgcatttc gctgcgcgct gaccctgttg gcagggctgg
     8581 cggtcctggc gttcgtgcgc gtcgagaaag tccgcgcggg agagacgcga caaccgctcg
     8641 cgattgaatt gtccttcgat cgtccgctgg aagcgagcat ggcccccttc gtcgtggcgg
     8701 agcgccgcgg actgttcgca gccgagaggc tctcggtcgc aaacaacttt gccgatggct
     8761 cggtcgacgc gatcgcacgc gtcgcctcgg gtgccagcga gtttgcgctc gtcgacctca
     8821 acgagttgat ccgctaccgc gacagggccg atgcgcaacc gatcaaggcg gtgtttgtgc
     8881 tgttcaacaa ggcgccttat gccatcgtgg cacgcaagag ccgcggcatt cgcgcgctct
     8941 ccgatgtcgt cggcaagacg cttggggtcg ccgagagcga cctgtcgttt cggctatggc
     9001 cggcgctcgc acggcaaaac ggcatcaggc cggaaagcgt gaaggtctac aggatgagtg
     9061 ccgcggtacg cgagccgatc ctgtccgcag gacaggtcga cgcggtcgcg ggcttctcct
     9121 atttgtcggc ggtgaacctg cgcgaccgcg gcgtccccgc aaacgacctc accgtgctgc
     9181 gttacgccga ctatggctgc gaggcctacg ggttcgcgat cgtcgtcaat ccgacctttg
     9241 cgtcggcaaa gccagatgcc gtgaaaggct ttgtgcgcgc tgtcatcgcg ggcacgcacc
     9301 ttgcgatcaa ggatccgcag cgtgcggtcg acgatgtggt cagcctgatg gacggcgcgt
     9361 cgcgcgaact ggagctagag cggctgcgca ccgtgattac cgacaacatc ctgaccggcg
     9421 aagtgaagcg ccacggcctc ggcggcatcg atcccgcccg cctcgaacgc tcgatcggcc
     9481 agatcgccga ggactacaaa ttcaggaagc ggccaacggc cgacgacgtc ttcgacggcg
     9541 gtttcctgcc accggtcggc gggcggctga tcaattgagc tgtatctcgc ccgcgatccc
     9601 tgattagaat gtagcgaacg cccaccctgc gtggtgggtc gcaagagtct ttgtatgtcg
     9661 ctcgtccgcc tctacatccg cgtcctcgag ctgctcggaa aagaggcgaa gctcggctgg
     9721 atcctcgcgg gcgcaaacct gctcctggcc gaggcgcaat ttgccgagcc cgtgctgttc
     9781 ggtcggattg tcgacgtact ctcgggacgg atggccccgg gcacttcggc gtggccgctg
     9841 cttgcggcct gggccgcctt cggactgttt acgatcgtag ccggcgcggc ggtagcgctg
     9901 tacgccgatc gcctcgccca tcgccagcgt caggccgtgc tgtcgagcta tttcgagcac
     9961 atcctgcaac tgcccctgac cttccacacc ggcacccatt ccggcaagct gatgaaggtg
    10021 atgctgaacg gcaccgacgc gctgtggcgg ctgtggctcg gcttcttccg cgaacatttc
    10081 gccgcgatca tgtcggtcgt ggtgctgctg ccgctgtcgc tttacatcaa ctggcggctt
    10141 gcgatcctgc tgttcgcgct ctgcgtggtg ttcacggtgc tgaccacgct cgtggtgcgc
    10201 aagacctacg gtatgcagaa cgaggtcgag gagcactata gcgatctctc cgcgcgcgcc
    10261 tccgacgcgc tcggcaatgt cgcgctggtg cagagcttcg tgcgcatcga tgccgaggtg
    10321 cagggcctgc gcttcgtcgc cgacaagctg ctcgccgcgc agatgccggt gttgtcctgg
    10381 tgggcgctgg tcaccgtgat cacccgcgcc tccaccacga tcacggtgct ggcgatcttc
    10441 acggtcggca tcgccttgca tcaggcgggc cttactacgg tcggcgagat cgtgatgttc
    10501 gtctcgtttg ccaccatgct gatccagaag ctcgagcagg tggtttcctt catcaacaac
    10561 gtgttcatgg aagcgccgcg cctgcgggaa ttcttcaacg tgctcgacgc cgtgcccgcg
    10621 gtgcgcgacc gccccgatgc gatcgaagtc ggacgactct ccggccttgt ggatttcaac
    10681 gacgtctcgt tttcctatga tggcaaacgc ccggctgtgg aagacctctc cttcaccgcc
    10741 ctgccgggcg agaccatcgc gctggtcggc ccgactggcg ccggcaaatc gaccgcgatc
    10801 gcgctgttgc atcgcgcatt cgatccgcaa tccggcttca tcaggatcga cggcatggat
    10861 gtgcgcgggc tgaagctgac ggcgctccgg cgcaatatcg gcgtggtgtt ccaggaggcc
    10921 atgctgttcg atcgctcgat cgcggacaat ttgcggatcg gcaagcccga tgccaccgac
    10981 gaggagctga ggattgcggc cgcgcgtgcg caggcgctgg atttcatcga gcgcaacgaa
    11041 cggggtttcg ataccaaggc cggcgagcgc gggcgcatgc tgtccggcgg cgaacggcag
    11101 cggctgtcga tcgcgcgtgc gctgctgaaa gacccgccga tcctgatcct ggatgaggcg
    11161 accagcgcgc tcgatgccgt caccgaggcc aaggtcaatg cggcgctaga cgaggtgatg
    11221 aaggggcgca ccacatttgt gatcgcccat cgcctctcca ccatccgcaa cgccacgcgc
    11281 atcctggtgt tcgacaatgg acgcgtgatc gaaagtggaa ctttcgatga actggtggcg
    11341 aaaggcggcc gtttcgcagc gcttgcgcgg gcccagttca tggtgcaggg ggacgcgcgc
    11401 gcaaataatg ctgtcaaggc gtaagtttcg ccgaaattcg gcccgcttcc gcgccgaatt
    11461 gcttacccag gacctctgtt ctgaacgacg cagccggtat aggtttatat cggctgtaag
    11521 ctcgtgccgt agattcgacg ctcgctgcag tgtcgcctct ttcctcaagg cgaatgtcgc
    11581 atccagcggc tcctcacaat gaccggaatc ggaatagtgc agttgtttca ccagttgctt
    11641 tccagatcac tgttgcgggg aattgctctc ctcgccgtgc tcgcaatctt cgcgccgcgc
    11701 gcacttcatg ccgaggcgcg acttgttgtc gaggccgata gcggaaaggt tctcgaggct
    11761 gagaacgcga ccgtgccctg gtatccggcc tccgtcacca agctgatgac cgcctatgtc
    11821 acgctgaggg cggtgaagga cggcagggtc acactcgata cgctctttac cgtctcgccg
    11881 gtcgcggcct cgcaatcgcc atcgaagatg ggcttcaggc cgggcacgca ggtcaccgtc
    11941 gacaatgcgc tcaagatgat gttggtgaaa tctgccaacg acatggccgt ggtgctcgcc
    12001 gaaggcgtcg gcggctcgat cgacggcttc tccgtcatga tgaaccagac tgcgcaaagg
    12061 ctcggcatga cgcagtcgca ttacgtgaac ccgaacggcc tgccggcgga cgagcaggtc
    12121 acttccgcgc gcgatcttgc catccttgcg cgcgcgatca tccgcgacct gcccgaatac
    12181 gaatatttcg tgcacatccc ctcgatccgt ttgggccaca ggatcaccca gaacttcaac
    12241 cggctgatcg gccgctatcc cggcgccga
//
LOCUS       DATLJT010001211         1926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2848358,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001211 DATLJT010000000
VERSION     DATLJT010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848358"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VM910_27960"
     CDS             <1..858
                     /locus_tag="VM910_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66385.1"
                     /translation="DVSFDLAKSEMVALIGHNGAGKTTLMKLMLGLIRCTRGSIEVLG
                     DNPAAGQFAGRRHLGYLPENVSFDAALTGRETQAFYARLKREPVAKALELLDMVGLGP
                     AAARRVGTYSKGMRQRLGLAQALIGRPWVLLLDEPTTGLDPELRQTFYEVIQRLAAEG
                     TSVLLSSHALTELEERAGRVIIMNHGIKVADGSIEELRRLARLPTKIRLKVSGLESDE
                     MPAWLPSGAPCRRLNGHIVEIDAAPEQKIDLLHRVTAEGSSVEDLDVVPPSLDELYAH
                     FLKSTEMPR"
     gene            855..1682
                     /locus_tag="VM910_27965"
     CDS             855..1682
                     /locus_tag="VM910_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI66386.1"
                     /translation="MSAIIVALKEIHQAVRNRWVLAATLLLAGLALSLTFLGSAPTGS
                     VGVRALDVVVVSLSSLTILLIPLIALLISHDSIVGDMERGTMLLLLSYPVARWQVLLG
                     KFLGHLAVLAFATCLGYGLAAAALMVTGSRVDNDSLVAFAAMIGSSVLLGAVFVAIGY
                     LISALARDRGTAAGVCIGLWLLLVLIYDMALLGLLALDQGRNISAGVLNAMLLFNPTD
                     VYRLFNLTGFANVSMFAGMAGLAQSTSLSAPALLAALMLWTFVPLGLAALAFSRREL"
     gene            1679..>1926
                     /locus_tag="VM910_27970"
     CDS             1679..>1926
                     /locus_tag="VM910_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HVI66387.1"
                     /translation="MRRLALAATLVAFALAGCDQKEAALPPRPVAMTKDAIGHYCGMN
                     LLEHPGPKGQIIAKSLIEPVWFSSARDAIAFTMLPDEP"
BASE COUNT          319 a          592 c          592 g          423 t
ORIGIN      
        1 gatgtttcct ttgaccttgc caagagcgag atggtcgcgc tgatcggtca caacggcgcc
       61 ggcaagacca cgctgatgaa gctgatgctg ggattgatcc gttgcacacg cggctcgatc
      121 gaggtgctcg gcgataatcc cgccgccggc cagttcgcgg gacggcgtca tctgggttat
      181 ttaccagaaa acgtctcctt tgatgccgcc ttgaccggcc gcgaaacgca ggccttctat
      241 gccaggctca aacgggagcc ggttgcgaag gcgctggagc tgttggacat ggtcgggctc
      301 ggcccggccg cggcgcggcg cgtcggcacc tattcgaagg gcatgcgtca gcgtcttgga
      361 ctcgcgcagg cgctgatcgg ccggccctgg gtcttgctgc tcgatgagcc aaccaccgga
      421 ctcgatccag aattgcgcca gaccttctat gaagtgattc aacgattagc cgccgaaggc
      481 accagcgttc tgttgtcgtc gcacgccttg acagaactgg aggagcgcgc cgggcgggtg
      541 atcatcatga accacggaat caaggtcgcg gacggatcga tcgaagagct gcgccgcttg
      601 gcgcggttgc cgaccaaaat ccggctcaag gtttccggcc tggaatccga cgagatgccg
      661 gcatggctgc cgtccggcgc gccctgccga cggttgaacg gccacatcgt cgagatcgac
      721 gcagcgcccg aacagaagat cgatctcttg caccgcgtga ccgcggaggg atcatccgtc
      781 gaggatctcg acgtggtgcc gccgagtctc gacgaactct atgcgcattt tctcaagtcg
      841 acggagatgc cgcgatgagc gccattatcg tcgccctcaa ggaaatccat caggcagtgc
      901 gcaatcgctg ggtgctcgcg gcgacgctcc tgctcgccgg gctcgctttg tcgctcactt
      961 ttctcggcag cgcgccgact ggcagcgtcg gcgtgcgggc gcttgacgta gtggtcgtga
     1021 gcctctcgag tctcacaatc ttgctcattc cactgatcgc gcttctgatc tcgcacgatt
     1081 ccatcgtcgg agacatggag cgaggaacga tgctgctgct cctcagctat cccgtcgcgc
     1141 gctggcaggt actgctcggt aaattcctag ggcatcttgc cgtgctcgcc tttgccacat
     1201 gtctcggcta tggattggcc gctgcggcgc tgatggtcac cggaagccgt gtcgataacg
     1261 atagtttggt ggcattcgcc gcaatgatcg gttcctcggt gctcctcggt gcagtattcg
     1321 ttgcgatcgg ctatctcatc agtgccctgg cgcgtgaccg aggtacggct gccggcgtgt
     1381 gcatcggcct gtggctgttg ctggtgttga tctacgacat ggcgcttctc ggtttgctcg
     1441 cgctggacca gggccgcaat atttccgccg gcgtcctcaa tgctatgctt ctgttcaatc
     1501 ccactgacgt ctatcggctc ttcaatctga ccggtttcgc caatgtgagc atgtttgcgg
     1561 gcatggctgg cctcgcccag agcacatctt tgtccgcgcc cgcgttactg gcggcactga
     1621 tgctttggac attcgttcca ctggggttgg ccgccttggc attttcgcgg agggagctat
     1681 gaggcgcctt gctctcgctg ccacacttgt tgccttcgcg cttgccggct gcgaccagaa
     1741 agaagcagcg ctgcctccgc gaccggtcgc catgaccaaa gacgcgatcg gtcattactg
     1801 cggtatgaat ttgctcgagc atcccggtcc caagggccag atcatcgcca agagtctcat
     1861 cgagccggtc tggttctctt cggcgcgcga tgcgatcgct ttcaccatgc tgcccgacga
     1921 gccgaa
//
LOCUS       DATLJT010001212         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_89938,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001212 DATLJT010000000
VERSION     DATLJT010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89938"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VM910_27975"
     CDS             complement(<1..609)
                     /locus_tag="VM910_27975"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66388.1"
                     /translation="MSSIKRREFITLLAGAAAAWPLAARAQKADRMRRIGVLMNTTAE
                     DPLGQAGSAAFAQGLQQLGWEVGFNVRVDTRWGAGDTERYRKYATELVALAPDVILAT
                     ANSIVSALQQAGRTVPIVFVTTIDPVGGGLVASLARPGGNATGFTSYEFSLNAKLLEL
                     LKEVAPGVTRVAVLRDPFVSAGSGGFAAIQTAAASLKVELIPI"
     gene            complement(862..1209)
                     /locus_tag="VM910_27980"
     CDS             complement(862..1209)
                     /locus_tag="VM910_27980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66389.1"
                     /translation="MIACLRRSRVGEKPIGLEQPDAEDERERDATLRRPHDARLRLER
                     ADRVFEGLQPLGADEIALVEQNDVAVTELVAGSLALELLEAKASGIGNRDDRIDAHPR
                     TWVTSGPNRFRRE"
     gene            complement(1254..1556)
                     /locus_tag="VM910_27985"
     CDS             complement(1254..1556)
                     /locus_tag="VM910_27985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66390.1"
                     /translation="MRKAGFELRSQLRAIEIATNQHELVPRLAFAFAFIQGESLGHEV
                     ENVAFVAFGNPKEALAAIDVIGKPLEEVLKSLRGKGPLALKRKASKPSAAKWSVTC"
     gene            1916..>2257
                     /locus_tag="VM910_27990"
     CDS             1916..>2257
                     /locus_tag="VM910_27990"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HVI66391.1"
                     /translation="MLRPAFINDNFIPVLVDRDERPDVDMLYQGAAGIMGHGGGWPLN
                     IFLSPQGVPFWVAGYQPKEDRPDNPSFARVARETAELWRNDRARAEDTGAKVTAAVEN
                     LYNRDMTQAAEP"
BASE COUNT          440 a          752 c          648 g          417 t
ORIGIN      
        1 gatagggatc aactctacct taagcgacgc tgctgctgtc tggatagcgg caaagccgcc
       61 tgacccggcg gatacgaagg gatcgcgaag gaccgctacg cgtgtcacgc cgggtgcgac
      121 ctccttgagc agttccagca acttcgcgtt taggctgaat tcatacgagg taaaaccggt
      181 ggcgttgccg ccgggccgtg ccaggctcgc gaccaagccg ccaccgaccg gatcgattgt
      241 tgttacgaac acgataggca cggttcggcc tgcttgctgc aaagcgctga cgatcgagtt
      301 ggccgtagcg aggatgacat ccggcgctaa cgcgaccaac tcagttgcgt atttgcgata
      361 ccgctcagta tcgcctgcac cccaacgagt atcgactcga acgttgaatc cgacttccca
      421 acccaattgc tgcaatccct gtgcgaacgc cgcgcttccg gcctgtccca aaggatcctc
      481 cgcggtcgtg ttcatcaaca cgccgatgcg ccgcatacgg tcggccttct gggcgcgcgc
      541 cgcgagcggc cacgccgccg ctgctccggc gagcagcgtg atgaactcgc gccgcttaat
      601 gctggacatg gggacttcct cccctgtcgg gtggcgtttg caccaccggg gccaaccgcc
      661 aggcgacgac gctcggttta ccgcagactc aacctgccgc aaaacggacg gcaagtcctt
      721 gggccagacc tgaatcgttc tgaatctagg cgggacgctg ccgacccccc tcctgtgttg
      781 ccacccaaac cgatagcaca cacgatgccc ccggagatcg ctgcactgcg ggattttcag
      841 tcgctcaatg tccgctttgg gtcattcgcg tcggaagcga ttagggccgc tcgtgaccca
      901 tgtccgcggg tgcgcgtcga ttcgatcatc acggttgccg atgccgctag cttttgcctc
      961 gagcagttcg agagcaaggc tgcccgcaac cagctctgtc acggcgacgt cattctgctc
     1021 aacaagtgcg atctcgtcgg cgccgagcgg ctgcaaaccc tcgaagacac gatccgcacg
     1081 ctcaagccgc aagcgcgcat cgtgcggacg acgaagtgtc gcgtcgcgct ccctctcgtc
     1141 ctcagcgtcg ggctgttcca atccgatcgg tttttcgccg acccgagacc ggcgcaggca
     1201 tgcgatcaag agttcgactc aaatcacgag agcgagcaac tgcacgccga tcatcaacat
     1261 gtcaccgacc atttggcggc ggacggtttc gaggcctttc gtttcaaagc gagcggccct
     1321 ttgccgcgca aagatttcag gacttcctcg agcggcttcc cgataacgtc tatcgcggca
     1381 agggcctcct ttggattgcc gaaagcaaca aacgctacgt tttccacctc gtggccaagc
     1441 gattctcctt ggatgaaggc gaacgcgaag gccaaacgag gaacaagctc gtgctgattg
     1501 gtcgcgatct cgatcgcgcg cagttgcgat ctcagctcga agcctgcctt acgcaagctc
     1561 cgaccgccct tgtttagcgg tctcgcgttg gatacatgcg gccactcaat atccgagttg
     1621 ggtcataagc agaccagcgg cataacttac gccaagtccg ctttgccccc aaaagcggac
     1681 atcgaccgac gcaatgggca tgtccgctta gtgccaatcg cgacctaatg caccgcagta
     1741 agcaaccgcc ctattcgatc accctgtcgg cgcacgccag cagcaccgac ggcacgtcaa
     1801 ggccgagtgc cttcgcagtc tcgaagctgc cgatgcgctc gaactcgcag acacacgagg
     1861 tcagcccatc agcagcttgc cggtcacctc gcgtcccatc gtcgtgatct gatccttgct
     1921 gaggccggcc ttcatcaacg acaacttcat cccggtgctg gtggaccgtg acgagcgtcc
     1981 ggatgtcgac atgctctatc agggcgcggc cgggatcatg ggccatgggg gcggctggcc
     2041 gctcaacata ttcctgtcgc cgcagggcgt gcccttctgg gtggcgggct accagcccaa
     2101 ggaggacagg ccggacaatc ccagctttgc ccgcgtcgcc cgcgaaaccg ccgagctctg
     2161 gcgcaacgac cgcgcccgcg ccgaggacac cggcgccaag gtcacggccg ctgtggagaa
     2221 tctctacaac cgcgacatga cgcaggccgc tgagccc
//
LOCUS       DATLJT010001213         4991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3997229,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001213 DATLJT010000000
VERSION     DATLJT010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997229"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /gene="ccoN"
                     /locus_tag="VM910_27995"
     CDS             complement(<1..1607)
                     /gene="ccoN"
                     /locus_tag="VM910_27995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HVI66392.1"
                     /translation="MTDASIGSKSMTIGEALLAPLFLVLAFLCVIGAAQAHDGAFAFH
                     ASLGAAGSLLAAFAIINHYYNRPAELPAQEINGRPNYNLGPIKFGAVAAVFWGVAGFL
                     VGLIIACQLAWPTLNLDLPWTSFGRLRPLHTSAVIFAFGGNVLVATSLYVVQKTCRTR
                     LAGYLAPWFVVLGYNFFIVVAGTGYLLGVTQSKEYAEPEWYADLWLTIVWVVYLLVFL
                     ATIIKRKEPHIFVANWFYLAFIITIAVLHLGNNPALPVSVVGSKSYIAWGGVQDAMFQ
                     WWYGHNAVGFFLTAGFLAIMYYFIPKRAERPIYSYRLSIIHFWSLIFLYIWAGPHHLH
                     YTALPDWAQTLGMTFSVMLWMPSWGGMINGLMTLSGAWDKLRTDPVLRMLVVSVAFYG
                     MSTFEGPMMSIKVVNSLSHYTDWTIGHVHSGALGWVGFVSFGALYCLVPWLWNRKGLY
                     SLKLVNWHFWTATLGIVLYISAMWVSGILQGLMWRSYTSLGFLEYSFIETVEAMHPFY
                     IIRAAGGALFVIGSLIMAYNLWMTVRAG"
     gene            complement(1862..2089)
                     /locus_tag="VM910_28000"
     CDS             complement(1862..2089)
                     /locus_tag="VM910_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66393.1"
                     /translation="MPSFDVRFIKTVCDDTGHEHRACQATFTVEASSLAEAVQRAEAD
                     FCRQKHVHDWTIFADAVECRSPTALARAWGG"
     gene            2144..2395
                     /locus_tag="VM910_28005"
     CDS             2144..2395
                     /locus_tag="VM910_28005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66394.1"
                     /translation="MTDIKSGISTNGPPGCQNGKTDNDDEDDGSSDDAEVKQVMPDKA
                     GGDHRRQGQQDSYDPQHGLSVYCSLKIIDTLQPRRAVGF"
     gene            2455..2598
                     /locus_tag="VM910_28010"
     CDS             2455..2598
                     /locus_tag="VM910_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66395.1"
                     /translation="MPVFTIAAIVAIFAAFAAALAWAQFHARPTDSLPAHAGLFRSKR
                     RPL"
     gene            complement(2636..3244)
                     /gene="fixJ"
                     /locus_tag="VM910_28015"
     CDS             complement(2636..3244)
                     /gene="fixJ"
                     /locus_tag="VM910_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HVI66396.1"
                     /translation="MSGKTVIHVVDDDAAMRDSLAFLLDVNGFQPKTYESANAFLSAA
                     ATGAASCVISDIRMPGLSGIELVRALKSRGEACPVILITGHGDVALAVEAMKAGAVDF
                     IEKPFDDAALLSAIRAALDTRSAKQGDSAAKKEAEARLADLSPRERDVLLGLVAGKIN
                     KVIAHDLNISPRTVEVYRANLMAKTGAHSMSELMRLALAAGL"
     gene            complement(3234..4676)
                     /locus_tag="VM910_28020"
     CDS             complement(3234..4676)
                     /locus_tag="VM910_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI66397.1"
                     /translation="MTTQSRFWFRYGTAAAAAILVFLLLFLLRQYFGDRTFMVIYMPV
                     VVYAAFAGGRGPAMLATVLCIGISLFFLGSDLFSNPANLIDVAFFGVLGVVLGFIGDR
                     LLRESEESRYRQAQLQSILDTVPEAMIVIDEHGIMRSFSTTAERLFGWSAAEAIGKNV
                     SMLMPSPYRQEHDDYLERYLTTGERRIIGIGRIVVGERKDGSTFPMELAVGEARVGAD
                     RFFTGFVRDLTERRKEERRMQDLQSELVHVSRLTAMGEMASSLAHELNQPLSAITSYL
                     RGAATLLKADEVDKGRVREALDRSADQALRAGDIIKRLREFVAKGETQQSVENPGVLL
                     EEAAALALVGAREQGVRVSLRCDRDLPDIVVDKIQIQQVALNLIRNAIEAMETTIRRD
                     LTVSVARQGELARFSVADTGSGISPEIAKHLFQPFVTSKANGMGVGLSICRTIIEAHG
                     GRISAHPNAGGGTVFEFTIPLADAEASHER"
BASE COUNT         1024 a         1531 c         1561 g          875 t
ORIGIN      
        1 ccggcgcgta ccgtcatcca gagattgtac gccatgatca gcgagccgat cacgaacagc
       61 gctcctcccg cggcacggat gatgtagaag ggatgcatcg cttcgacggt ctcgatgaag
      121 gaatattcga ggaagccgag cgaggtgtag gaccgccaca tcaggccttg caggattccc
      181 gatacccaca tcgccgagat gtagagcacg atgccgagtg ttgcggtcca gaagtgccag
      241 ttgacgagct tgaggctata gaggcccttg cgattccaca gccacggcac gaggcaatag
      301 agcgcgccaa acgagacgaa gccgacccag cccagcgcac cggagtgtac gtggccgatg
      361 gtccagtccg tataatggct gagcgaattg acgaccttga tcgacatcat cggcccctcg
      421 aaggtcgaca tgccgtagaa ggcgaccgac accacgagca tgcgcagcac cgggtcggtg
      481 cgcagcttgt cccaggcgcc cgacaacgtc atcaggccgt tgatcatgcc gccccaggag
      541 ggcatccaca gcatcaccga gaaggtcatg ccgagcgtct gcgcccaatc cggcagcgcg
      601 gtgtaatgga ggtgatgcgg gccggcccaa atataaagga agatcagcga ccagaagtgg
      661 atgatggaca gccggtagga atagatcggg cgctcggcgc gcttcggaat gaagtagtac
      721 atgatggcga ggaagccggc ggtcaggaag aagccgaccg cattatggcc gtaccaccac
      781 tggaacatcg catcctggac gccgccccag gcgatgtagg atttcgagcc gaccaccgag
      841 accggcagcg cgggattgtt gccaagatgc agcaccgcga tggtgatgat gaaggcgagg
      901 tagaaccagt tggcgacgaa gatgtgcggt tccttgcgct tgatgatcgt cgcgaggaac
      961 accagcagat agaccaccca gacgatggtg agccacaggt cggcatacca ttccggctcg
     1021 gcgtattcct tgctttgcgt aacgccgagc agatagccgg ttccggcgac cacgatgaag
     1081 aagttgtagc cgagcacgac gaaccagggc gcaaggtagc cggcgagccg cgtacggcag
     1141 gtcttctgca cgacatataa tgacgtcgcg accaggacat tgccgccgaa cgcgaagatc
     1201 accgccgagg tatgcagcgg ccggaggcgg ccgaagctgg tccaaggcag gtccaggttg
     1261 agcgtcggcc aggcgagttg gcaggcgatg atcaggccga ccaggaatcc ggcgacaccc
     1321 cagaataccg cggcgaccgc gccgaatttg atcggcccga gattgtagtt gggcctgccg
     1381 ttgatctcct gtgccggcaa ctcggccgga cggttgtaat aatgattaat gattgcgaac
     1441 gcggccaaca ggcttcctgc agcgccgaga gacgcatgga aggcaaaggc gccgtcgtgc
     1501 gcctgggcgg ccccgatcac gcacaggaac gcgagcacaa gaaacagcgg cgcgagcagc
     1561 gcctcgccga ttgtcatcga cttcgaacca atggacgcat ctgtcatgag ctgcgacttt
     1621 ccttctttgc ttgccgcgtc gagggaaaca cgggcgcggc gtcggcgatt tgaagaggat
     1681 caggttccgc agtggatggc gcgcgcaggt ccgtcgaacg gtttgcttca cgccggccga
     1741 aacaaccccc tggtttgctg tcgtaaggct tcgccccgcc gcgctgcttg atccatgtca
     1801 agccagggtc ggttccgaaa gcggcacggg tgcaaaaaaa tcgcgttggg cagcgagcgc
     1861 gctagccgcc ccatgcccgt gccaatgcgg tcggcgaacg gcattccacg gcgtcggcga
     1921 agatcgtcca gtcatggacg tgtttttgcc tgcagaaatc ggcttccgcc cgctggacag
     1981 cttccgccag cgaactcgcc tccacggtga atgtggcctg gcaggcacgg tgctcgtgcc
     2041 cggtgtcgtc gcacacggtc ttgatgaagc ggacgtcgaa tgagggcacg gcgattctcc
     2101 agttcagctt gtcaaagaat gggaccaagc cgcaggcgtc gccttgaccg acatcaaatc
     2161 agggatctcg acgaatggtc cacccggttg ccagaacggc aaaaccgata atgacgacga
     2221 agacgacggc agttccgacg atgccgaagt caagcaggtc atgcccgata aagcgggcgg
     2281 cgatcatcgc cggcagggcc aacaggattc gtatgatcca cagcatggtt tgagcgtcta
     2341 ttgcagcctg aaaatcatcg acactctaca gcctcggcgt gcggtcggat tttgatgcag
     2401 gtcaaggcgg cgcgtcaacg attccgccaa tgttacaatt gaggagttgc tcccatgccc
     2461 gtattcacca ttgccgcaat cgtcgcaatc tttgccgcct tcgctgccgc gctcgcctgg
     2521 gcacaattcc atgcgcgccc gaccgacagc ctgcccgccc atgcgggcct gttccgttca
     2581 aagcggcgcc ctctttaagg catgacccgg cacgagaagt cgaaattagt ctcgtctaga
     2641 gacctgctgc cagcgcgagc cgcatcagtt ccgacatgct gtgcgcgccg gtcttggcca
     2701 tgaggttcgc gcggtaaacc tcgacggtgc gcgggctgat gttgaggtca tgggcgatga
     2761 ccttgttgat ctttccggcc accaatccga gcagcacgtc gcgttcgcgc ggcgacaggt
     2821 cggccagccg cgcctccgct tcctttttcg cggcactgtc gccctgcttc gccgaccggg
     2881 tatcgagcgc ggcgcggatg gcggagagca gcgccgcatc atcgaacggc ttctcgatga
     2941 aatcgacagc accggccttc atcgcttcga cggcaagcgc gacgtcgccg tggccggtaa
     3001 tcaggatcac cggacacgcc tcccccctgc tcttgagcgc gcgcacgagc tcgatgccgc
     3061 tcaaacccgg catgcgaatg tccgagatca cgcagctcgc agcaccggtt gcggcagccg
     3121 acaggaacgc attcgccgat tcataggttt tgggctgaaa gccgttgacg tcgagcagga
     3181 atgcaagcga gtcacgcatc gccgcgtcgt cgtcgacgac atggatgacg gtcttaccgc
     3241 tcatgacttg cctccgcatc ggcgagtggg atcgtgaatt cgaaaacagt gccgccaccg
     3301 gcattgggat gggccgagat ccggccgcca tgtgcttcga tgatcgttcg gcagatcgag
     3361 agacctacgc ccattccgtt tgccttgctg gtaacgaagg gttgaaacag atgttttgcg
     3421 atctcggggc tgattccgct gccggtatcc gccaccgaga accttgccaa ttcgccctgg
     3481 cgcgcgaccg agaccgtcag atcgcggcgg atcgtggtct ccatcgcctc gatggcattg
     3541 cggatcaggt tcagcgcgac ctgctgaatc tggatcttgt cgacgacgat gtcgggaaga
     3601 tcgcggtcgc agcgaagcga cacgcgcacg ccctgctccc tcgcgccgac cagagcaagc
     3661 gccgccgcct cttccagcag caccccagga ttctcgaccg actgctgggt ttctcccttg
     3721 gcaacgaatt ccctcagccg cttgatgatg tcgccggcgc gcagtgcctg gtcggcgctg
     3781 cggtcgagcg cctcccgtac gcgacccttg tcgacctcgt cggccttgag cagcgtggcg
     3841 gcgccgcgaa gatagctggt gatcgccgac aggggctggt tcagctcatg cgccagcgac
     3901 gacgccatct cgcccatcgc ggtgagacgg gagacgtgga ccagttcgga ttgcaggtcc
     3961 tgcatgcgcc gttcctcctt ccgccgctcg gtcaggtccc gaacgaagcc ggtgaaaaag
     4021 cggtcggcgc cgacccgggc ctcgccgacc gccaattcca tggggaacgt cgagccgtcc
     4081 ttgcgctcgc cgaccacaat gcgcccgata ccgatgatgc ggcgctctcc cgtggtcaga
     4141 tagcgctcga gataatcgtc gtgctcctgt cggtagggcg aaggcatcag catcgagacg
     4201 tttttcccga tcgcttcggc ggctgaccag ccgaacaggc gctcggcggt cgtgctgaac
     4261 gatcgcatga tgccgtgctc gtcgatcacg atcatcgctt ccggcacggt gtcgaggatc
     4321 gactggagct gggcctgccg gtagcgggac tcctctgatt cgcgcaacag gcggtcgccg
     4381 atgaagccga ggacgacgcc cagcacgccg aagaatgcga catcgatcag atttgccggg
     4441 ttggaaaaga gatcgctgcc caggaagaac aggctgatgc cgatgcacag gacggtggcc
     4501 agcatcgccg ggccgcgacc gccggcaaag gcggcataga ccaccacggg catgtaaatg
     4561 accatgaagg tgcggtcgcc aaaatactgg cgcagcagaa agagcaggag gaataccagg
     4621 atcgcggcag cggccgccgt gccgtagcga aaccagaagc gggattgtgt tgtcatccaa
     4681 agatccggtc cgactccgat ttgacgctct tgcacctttg ctctcggccc catttgttcg
     4741 gcggatcagg ccttccggca tgccactgct agcacagccg ccggaccttc gggttttgcc
     4801 ccttttttct ccaccgcgat catgccagaa attgcccggt ggcgagtacc caggagccga
     4861 gacgggccat ggataaaact gtggcggacc tgaacatcaa gcatttcaag aagctattgg
     4921 cgagcgagac ggacccggtc aagcggcaaa caatcgaacg cctgctcgcc gaggaagaag
     4981 ccaagctggc t
//
LOCUS       DATLJT010001214         1588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2619686,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001214 DATLJT010000000
VERSION     DATLJT010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619686"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1176
                     /locus_tag="VM910_28025"
     CDS             181..1176
                     /locus_tag="VM910_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVI66398.1"
                     /translation="MFGFDIFAIVFVLLVIVTLFAGIKTVPQGYDWTIERFGKYTNTL
                     SPGLNIIVPFFDRVGRKMNMMEQVINIPEQEVITKDNATVTVDGVAFFQVFDAAKASY
                     EVANLGQAITVLTMTNIRSVMGSMDLDQVLSHRDEINERLLRVVDAAVSAWGVKVNRI
                     EIKDIVPPADLVEAMGRQMKAERVKRADILQAEGQRQSEILRAEGAKQSQILQAEGRR
                     EAAFRDAEARERSAEAEAKATQMVSDAIAKGDVAALNYFIADKYIKAFGQFADGPNQK
                     VIMLPVEAVSMLGSLAGIGEIAKATFGESAVSAAAAARRASVPPAGPNPPPVTSQ"
     gene            1256..>1588
                     /locus_tag="VM910_28030"
     CDS             1256..>1588
                     /locus_tag="VM910_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HVI66399.1"
                     /translation="MTEMFATLGTWNWLIFGFVVMALELLAPGVFLFWFGLAALLVGL
                     VSFVLHPSWQMQLLMFAVFALIAVPVWRRLARSQREASESNPFLNRRAAALVGRVFTL
                     EKPIVDGFG"
BASE COUNT          295 a          474 c          522 g          297 t
ORIGIN      
        1 agttgtgcat agctattctt gcaagaacag ccagtggtcc gatggcaaca ttcgtactcc
       61 atataggagg cacatttgcg aatgttggaa tcaggcgcca aagcaaagcg aaacggcgtc
      121 cgcgagcaat ttccggcggg aggctcgcgc gggcacagca tcacccgcct cggaggcttt
      181 atgttcgggt tcgatatctt cgccatcgtc ttcgtgctgc ttgtcatcgt cacgctgttc
      241 gcgggcatca agacggtgcc gcagggctat gactggacga tcgagcggtt cggcaaatac
      301 acaaatacgc tgtcgccggg cctcaacatc atcgtcccgt ttttcgaccg cgtcggccgc
      361 aagatgaaca tgatggagca ggtgatcaac attcccgagc aggaggtgat caccaaggac
      421 aacgccaccg ttaccgtcga cggcgtcgcg tttttccagg tgttcgacgc ggcgaaggcg
      481 agctacgaag tcgccaatct cgggcaggcg atcacggtcc tgaccatgac caatatccgc
      541 tcggtgatgg gctcgatgga cctcgaccag gtgctgtcgc atcgcgacga aatcaacgag
      601 cggctgctgc gcgtggtgga cgccgcggtc tcggcctggg gcgtcaaggt caaccgcatc
      661 gagatcaagg atatcgtgcc cccggccgac ctcgtcgaag cgatgggccg gcagatgaaa
      721 gccgagcgcg tcaagcgcgc cgacattctg caggcggaag gccagcgcca gtccgaaatc
      781 ctgcgcgccg agggcgccaa gcagagccag atcctgcagg cggagggccg ccgcgaggct
      841 gccttccgcg acgccgaggc gcgcgagcgt tctgcagagg ccgaggccaa ggcgacccag
      901 atggtctccg atgccattgc caagggcgat gttgcggcgc tcaactattt tatcgccgac
      961 aagtacatca aggcgttcgg gcagtttgcg gacgggccga accagaaggt gatcatgctg
     1021 ccggtggagg ccgtgagcat gctcggctcg ctcgccggca tcggcgagat tgcgaaggcg
     1081 accttcggcg aaagcgcagt gtcggcggcc gctgccgcgc gtcgcgcctc ggtgccgccg
     1141 gccgggccca atccgccgcc ggtgacgtcg cagtaacagg aggcatgagc atgatccgga
     1201 aaagtggtga cccgctttcc gaaaccgatc atgctccagg aaagagcatc gtgtcatgac
     1261 cgagatgttt gcaacgctgg gtacatggaa ctggctgatc ttcggcttcg tggtgatggc
     1321 gctggagctc ctggcgccgg gcgtcttcct gttctggttc gggcttgccg cgctcctggt
     1381 tggcctcgtg tcgttcgtgc tgcatccgtc ctggcagatg cagcttctga tgtttgcggt
     1441 attcgcgctg atcgcggtcc cggtgtggcg acgccttgcg cgcagccagc gcgaagcgag
     1501 cgagagcaat ccattcctca acagacgcgc cgcggcattg gtcggccgcg tgttcacgct
     1561 ggagaagccg atcgtcgacg gtttcggc
//
LOCUS       DATLJT010001215         1903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3997431,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001215 DATLJT010000000
VERSION     DATLJT010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997431"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1240
                     /locus_tag="VM910_28035"
     CDS             173..1240
                     /locus_tag="VM910_28035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66400.1"
                     /translation="MTEQWNKYAATAARKHGKPGREVRPKSMTVDIHSHVAIPQATAF
                     VKPHLDMSTIPLAHFSNAETKALAQKQDADVRPRITGYDERLADLDAMGIDVQLVMPP
                     PNQCFYTVPLDIAVKAARMVNDGLAEYVARKADRFVALGTVPLIDGAEAAKELERCMT
                     SLGMKGAEILTNVAGRELSEPAYAPFWQKAEALGALVVIHPNGFTDAKRLTRFYFNNV
                     IGNPLDTTIALHYLIFDGVLERHPKLKILAVHGGGYLPAYSGRIDHAWGARSDAHGDL
                     PHPPTSYLKKIYFDTVVFTPHQLAELVRLNGADHVIMGTDYPFDMAEYDPIGHVASAD
                     GFNAATIAAIAGGNARKLLGI"
     gene            complement(1513..>1903)
                     /locus_tag="VM910_28040"
     CDS             complement(1513..>1903)
                     /locus_tag="VM910_28040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66401.1"
                     /translation="AGHIQLMFISVGSAVPQWKADKVKLIAVGAAKRMALLPEVPTVA
                     ESGLPGYEAVSWFGLFGPPGMPADIVVKVNAEVRKTFADPDIKKNFLERQYFESIAGT
                     PEQLAKYIKSDEPKWRKVIHDAKVKGE"
BASE COUNT          356 a          659 c          554 g          334 t
ORIGIN      
        1 gagcagaaca tcaagctcac cctcgacctc gccgacgatg tcgtcatcat caacaccggt
       61 cgcgtggtgt ttcgcggcac cgccgatcag atcaagcttg acgacgccat tgtgtcgcag
      121 cacttgggtg tgttttgaca atcgccaact tcaggaaaac catggaacag caatgaccga
      181 gcaatggaac aagtacgcag cgaccgcagc gcgcaagcac ggcaaaccgg gccgcgaggt
      241 gcggccgaag agcatgaccg tcgacattca ctcccacgtc gccatcccgc aggccaccgc
      301 cttcgtcaag ccgcacctcg acatgtccac gattccgctg gcgcattttt ccaatgccga
      361 gaccaaggcg ctggcccaaa agcaggacgc ggacgtgcgc ccgcgcatca ccggttatga
      421 cgagcgcctc gccgatctcg acgccatggg catcgacgtg cagctcgtga tgccgccgcc
      481 gaaccagtgt ttctataccg tcccgctcga catcgcggtc aaagccgcgc gcatggtcaa
      541 cgacggactg gcggaatacg tggcgcgcaa ggcggaccgc ttcgtcgcgc tgggcaccgt
      601 gccgctgatc gacggcgcgg aagcggcaaa agagctcgag cgctgcatga cgtcgctcgg
      661 catgaaaggc gcggaaatct tgaccaacgt tgcggggcgg gagctctccg agccggccta
      721 tgcgccgttc tggcagaagg ccgaggcgct cggcgcgctc gtcgtcatcc accccaacgg
      781 cttcaccgac gccaaacggc tcacgcgctt ctacttcaac aacgtgatcg gcaacccgct
      841 cgacacgacc atcgcgctgc actacctgat cttcgacggc gtgctcgaac ggcacccgaa
      901 gctgaagatt cttgccgtgc acggcggcgg ctatctcccc gcttacagcg gccgcatcga
      961 tcacgcctgg ggtgcgcgct cggatgcgca cggcgacctg ccgcacccgc ccaccagcta
     1021 tctcaagaaa atctatttcg acaccgtcgt gttcacgccc caccagctcg ccgagctggt
     1081 gcgcctcaac ggcgccgacc acgtcatcat gggaacggac tatcccttcg acatggccga
     1141 gtacgatccc atcggccacg tcgcgtcggc agacggcttc aacgccgcga ccatcgccgc
     1201 catcgccggc ggcaatgccc gcaagctttt gggaatttga ttctcgcgcg tgcggcgatc
     1261 tcgatgcgct ccctctcccc gcttgcaacc gaactcgggt ttacccgagt tcggtcgcta
     1321 ttatgaagcg gcagaagtcg gataatatcc gacttctgct gagagggttg gggtgagggg
     1381 gatttcacct aagctcggtt cgaggagaat ccccctcacc cgctcgctga gctcgcgacc
     1441 tctgcccgca agcggggaga ggtggagcgc gcggcatgtg cgacgatccc catctttaga
     1501 ccctcggccc gctcactccc ccttcacctt ggcgtcgtgg atgaccttgc gccacttcgg
     1561 ctcgtcggac ttaatgtatt tcgcgagctg ctcgggcgtg ccggcgatcg attcgaaata
     1621 ctgccgctcg aggaaattct tcttgatgtc gggatcggcg aaggtcttgc gcacctcggc
     1681 gttgaccttg acgacgatgt ccgccggcat cccgggcgga ccgaacaacc cgaaccagga
     1741 caccgcctcg taaccgggca gcccgctctc cgccaccgtg ggcacttccg gcagcagcgc
     1801 catgcgcttg gcggcgccga ccgcgatgag cttgaccttg tccgccttcc actgcggcac
     1861 ggcgctgccc acgctgatga acatcagctg gatgtgcccg gcc
//
LOCUS       DATLJT010001216         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3997435,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001216 DATLJT010000000
VERSION     DATLJT010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997435"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VM910_28045"
     CDS             <1..694
                     /locus_tag="VM910_28045"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HVI66402.1"
                     /translation="GSDGAMITGNMIAGVKSVAGALGIGIELDSSGTSRHTITGNVIK
                     QCEGSAIVGEGGNHDVVVSGNVIDGTNVAVPDASIVFHSADRVTITGNKILNNRMGSP
                     IAMKGSSNHWLIAANSFAENNNNSPAPVSVDSTVINNNGYNPVGIIATPWQPSGELTN
                     NEGGSADPVSGKLYTVKQSPKTIIIAGGAVTQIAIDGTPTGLPAGVFKLGIGETIAVT
                     YSSTPTSRVAAD"
     gene            901..3068
                     /locus_tag="VM910_28050"
                     /pseudo
     CDS             901..3068
                     /locus_tag="VM910_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497463.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
     gene            3476..>3909
                     /locus_tag="VM910_28055"
     CDS             3476..>3909
                     /locus_tag="VM910_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI66403.1"
                     /translation="MLTLARRGGELDALVRAGRWAERLTAVPVDLLGKTVLVVGFGRT
                     GTRVAKRCLAMEMTVIVYDPYLPAETMRAAGCEPVSDLDAALPRADFVTIHCPRSAET
                     IGMFGAARLQRMKKTAYLVNTARGGIVDEGALHAALTAGTIAG"
BASE COUNT          826 a         1160 c         1205 g          718 t
ORIGIN      
        1 cggatccgac ggagcgatga tcacggggaa tatgatagcc ggcgtcaagt cggtcgcagg
       61 agcccttgga ataggaattg aactggattc ttctggcacc tcgcgccaca cgataacagg
      121 caatgtgatc aaacagtgcg aaggctcggc gatcgtagga gaaggcggga accacgatgt
      181 cgtagtgagt gggaatgtca tcgacggaac gaacgtggca gtgcccgacg cgagcattgt
      241 tttccatagc gctgacaggg ttacaatcac aggaaacaag attctcaaca accggatggg
      301 gagcccaatc gcaatgaagg gcagctccaa ccactggctg atcgcagcca actcatttgc
      361 tgaaaacaac aataacagtc ccgcaccagt gagcgtcgac tcgactgtca tcaacaacaa
      421 cgggtacaac ccagtcggga tcatcgccac tccctggcaa ccaagcggag agctgactaa
      481 caacgaagga ggcagcgcag atccggtgag cggaaagctc tacacagtca aacagagccc
      541 gaagacgatt attattgcgg gtggcgcggt gacacagatc gcgattgatg gaaccccgac
      601 ggggctcccg gccggggtat tcaaattggg aatcggcgaa actatagcag tcacctatag
      661 ctcgacgcca acgagcagag ttgctgcgga ttgatgccag ctattagaaa tgtggtttac
      721 cggtgattca aaccccggta gcggagggct gcctagtgaa agatcactac gcgcttacgg
      781 acggcgggac ccgcctcgct tgcatgagca ttgtcgttcc agcagccttc tggttatttt
      841 acgccccgcc gatctaatct cgtgttgcaa gagcgactct caatggcgcg cgacctacat
      901 atgaattacg tccggatcgg cagcttgagc atcgccaagg ccttgcatga gttaatcgaa
      961 caggaggcag cgccgggaac gaacatctct ggtgaagcgt tctggagcgg ctttgccgaa
     1021 ttgctgcgcg actgcggcac gcgcaatcgc cagctgcttc aggttcgcga cgagctccag
     1081 tcccgcatcg atcaatatca tcgtgagcgc tacggcgagc cacttgatct gagcgattac
     1141 gagtggtttt tgcgggacat cggctatgtt ttgcccgaag ggaccgactt tgctatccgc
     1201 accactaatg tcgatgacga gatcgcggta attgccgggc cccagctcgt cgtgccgctc
     1261 tccaacgcgc gatatgcgtt gaatgccgca aacgcgcgct ggggcagcct ctacgatgca
     1321 ctttatggca cggatgccat cccggaggac ggcgacacca cccgcacaag tacgtacaac
     1381 aagatacgcg gtgagcgagt cgtggcgcgc gggcgcgcca tgctcgatga gaccgtgcca
     1441 ctcgcccgcg gcagccaccg tgacgtcgac tcctactcag tgactcctac tcagtagaag
     1501 acgcggattt ggtggtgcga ctgcgggacg gcaagagcac cagccttgcg cggcccgccc
     1561 aattcaccgc gtatcggggt gaggcgagcg caccatcggt ggtgctgctg cgcaaccacg
     1621 gtcttcacct ggagatcaag atcgatcggg gcggacttgt cggacgcgat gatccggcga
     1681 gtatcgccga cattgtgttg gaatcggcgg tcaccaccat catggatctc gaagacagcg
     1741 tcgccgcggt cgatgcggag gacaaggtcg cggtctatcg caactggttg ggtctaatcg
     1801 aaggcacgct cacggcaaca ttccagaaga gcggaagaac agtgcaccgc caactcaatc
     1861 cggaccgggt ctatactgct cccggaggcg gcgagctgcg gctgccaggg cgtagtctca
     1921 tgctggtgcg cattgttggt catcacatgt acacggatgc ggtcctcgac gcctccgaca
     1981 acgaggtacc ggagggcata ctcgatgcag cggtgacggc gctcattgcg ctgcgcgatg
     2041 tcgccggccc gaatgccaag cgcaacagtc gaaccggctc gatctacatc gtgaagccga
     2101 agatgcacgg accggacgag gtggcctttg ccaatgagct gttagcgcgc gtggaagaca
     2161 tgctgggctt gccgcgctac accctgaagg tcggcatcat ggacgaggag cggcgcacca
     2221 gcgtcaattt aaagcaatgc atccgcgccg tggccgatcg cgcagtcttc atcaacaccg
     2281 gcttcctcga tcggactggc gacgagattc acacctcgat cgaagcgggg ccaatggttc
     2341 gcaagaccga gatgaagaac acgacctgga tcaaggccta cgaagattcc aacgtggatg
     2401 tgggtctcgc ctgtggactg cccagccgcg ctcagattgg caaggggatg tgggcggcac
     2461 cggacaagat ggcggatatg ctggcggaga agatcgctca tccgatggcg ggcgccaaca
     2521 ccgcctgggt gccttcgccc accgcagcaa cgctgcatgc gctccactat caccaggtcg
     2581 atgttgctgc ccggcagcac gagctgagag gccggccgcc gccgtcgctc gctgctctgt
     2641 taacgatccc gcttgggcgc tcaaattttt cacctgagga ggtgcagctg gagctcgaca
     2701 acaactgcca gggcatcctc ggatacgtgg tgcgttggat cgaccagggc ataggctgct
     2761 ccaaggtgcc ggatattcac gacgttgcct tgatggagga ccgtgcaacc ttgcgcattt
     2821 caagccagca catcgccaac tggctgcaac acggcatagt cgacgagcag caggtgttgc
     2881 ggacgctgcg gcggatggcc gagattgtcg accgtcagaa tgcgggcgat cccgcctatc
     2941 gactgatggc gtccggattt gacggccctg ccttcaacgc cgcccgcgac ctgatcttca
     3001 agggacgggc gcagccgaac ggctataccg aatggatttt gcaccggcgc cggcgcgagg
     3061 cgaaggcggg cggcacgccg accaatctgg gcgggtgggg cgccgacgga atgaccgcgt
     3121 cttctgctgc tcgttaccgg acgccaccta acgcgtcatg gcgtctgcca taagggcgag
     3181 atttcttgta gaaattcgcc tgatcgcgcg ccagaccggc ttcacatgag tagttaggcg
     3241 atatccccct taaaagccgt gttatgcttc gggaaccaca cgtgtccttt gagcatgtag
     3301 ttccagtaca tcagcggcag gcccgtcacc ttgagatcca ccagccgatc caatgttcct
     3361 tcgcggggtt gagcggcagc gtcggcgtca ccttcccgcc atagataaac tcggcgagaa
     3421 tagcttggtg gggactgcga actcctcctc ggtcgccgag cacgcgcttt tcctcatgct
     3481 cacgcttgca agacgaggag gcgagcttga cgcgctcgtg cgcgccggcc gctgggccga
     3541 acgcctgacc gcagtgccag tcgacctcct cggcaagacc gtgctcgtcg tcggctttgg
     3601 ccgtaccggc acgcgcgtcg ccaagcgttg ccttgcgatg gaaatgacgg ttatcgttta
     3661 cgatccctat ctaccggcgg aaaccatgcg ggcggccgga tgcgagccgg tctccgatct
     3721 cgatgccgcg ctcccgcggg cggatttcgt gaccatccac tgcccacgct ccgcggagac
     3781 gatcggaatg ttcggcgcgg cgcggttgca gcgcatgaaa aagaccgcct atctcgtcaa
     3841 caccgcgcgc ggcgggattg tcgatgaagg ggcactgcat gcggcgctga ccgcaggcac
     3901 catcgccgg
//
LOCUS       DATLJT010001217         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1469970,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001217 DATLJT010000000
VERSION     DATLJT010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469970"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VM910_28060"
     CDS             complement(<1..281)
                     /locus_tag="VM910_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI66404.1"
                     /translation="MFKSILVPIDLADTDLAKPAIATAATLSQTWNGVVRLLNVLPMT
                     PVMLAEYVPADFDAQQQQISEEALAIVARESGIEPSRISTAVRQGGIYH"
     gene            complement(361..903)
                     /locus_tag="VM910_28065"
     CDS             complement(361..903)
                     /locus_tag="VM910_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HVI66405.1"
                     /translation="MRRLMLFRHAKTETDAPSGRDQDRRLDERGHRDAAEVGGWIGRH
                     PPFPDLVQVSPAVRARQTWEIAWEAMKGHAPLPPVELVDELYGADPAQLLRTIRMAEA
                     SDPKRLMLVGHNPGMHELALALAGSGDAAARKALADNLPTAGLAVFDFATNDWTDVAF
                     RRGRLVLFVTPKLLKQASSD"
     gene            1164..>2012
                     /locus_tag="VM910_28070"
     CDS             1164..>2012
                     /locus_tag="VM910_28070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVI66406.1"
                     /translation="MNEISTGIVSGPDAAAPRERARLTFDLDVDICIVGAGLAGLTVA
                     LEASRLGASVAVLEGRHVGWGASGHQLGTVMPGYGLPIADLIERIGFEDTKALWALSR
                     EGADYVRAHATEESMPGIGLTEGALEVSNVDVGDQLISRLQMLSEDFETEVQGWQVDR
                     VRDQLRTDRYFHGIYHPKAFQIDGRKYVHGLAALARRAGARIFEDTPVVSVDSSGIRK
                     RVVTPSARLRASHIVLAGNVHLGAPLRRLSETLLPVWRFAGVTAPLGERLAEVIAFKG
                     SVNDTDN"
BASE COUNT          340 a          628 c          658 g          386 t
ORIGIN      
        1 tcgtgataga tgccgccctg tcggaccgca gtcgagatgc gcgagggctc gatgccggat
       61 tcgcgcgcga cgatggcgag cgcctcttcc gaaatctgct gctgctgggc gtcgaaatcg
      121 gccggcacat attccgccag catcaccggc gtcatcggca gcacgttgag gagccgcacc
      181 acgccgttcc aggtctgcga cagcgtcgcg gcggtcgcga tcgcgggctt tgccaggtcc
      241 gtgtcggcaa gatcgatcgg cacgaggatg gatttgaaca tctgcgcctc ctggtgtcgc
      301 gaacttcgct ccattttgct gcgaacttcg cgagcctagc agaaacggcg cggtcccgtc
      361 tcagtcgctg gacgcctgct tcagcagctt tggggtgacg aagagaacca gccgcccgcg
      421 gcggaacgcc acatcggtcc agtcgttggt ggcaaaatcg aacacggcga gccccgccgt
      481 cggcaggttg tcggcgagcg ccttgcgtgc ggcggcatcg ccgctaccgg ccagcgccag
      541 tgccagttca tgcatcccgg gattgtggcc gaccagcatc aatcgcttcg gatcgcttgc
      601 ctccgccatg cggatggtcc gcaagagctg cgccgggtcg gcgccgtaaa gttcgtccac
      661 caattcgacc ggcggcagtg gcgcatgacc cttcatcgcc tcccaggcga tttcccaggt
      721 ttgccgtgcc cgcaccgccg gcgacacctg tacgaggtcc ggaaacggcg gatgccggcc
      781 gatccagccg ccgacctcgg cagcgtccct gtggcctctt tcgtcgagcc gacggtcctg
      841 atcgcggccg gagggcgcgt ctgtctcggt cttggcatga cgaaagagca tcaaacggcg
      901 catgggtacg ggttctcgat tcggatcttg cctcaattag tctacaagcc tgcgcataga
      961 acaaggtgac aggcaggccg gtcgccgaaa tccgatgttc gcaggatttt ggacctgtcc
     1021 tcttggctaa cttatgattc gaagggacac tcgcaaatcg ctgttgtggt gccgcttttc
     1081 aacgcgaggt tcctgttccg ggtgccggca atctgtgcac gaacttcaaa tcggtggcac
     1141 cgggggatta acgagaagac gatatgaacg agatttccac aggcatcgtc agtggacctg
     1201 atgccgcagc gccgcgggag cgcgcgcggc tgaccttcga cctcgacgtc gatatctgca
     1261 tcgtcggcgc gggattggcc ggcctgacgg ttgcgctgga ggcgtcgcgg cttggagcca
     1321 gcgtcgccgt gctggaaggc cgacacgtcg gctggggcgc atccggccat cagctcggca
     1381 cagtgatgcc gggttatggc ctgccgatcg ccgacctgat cgagcggatc ggttttgaag
     1441 acacgaaggc gctatgggcg ctttccaggg agggcgccga ttacgttcgc gcccatgcga
     1501 ccgaggaatc gatgcccggc atcggcctga ccgaaggggc gcttgaagtc tccaatgtcg
     1561 atgtcggcga ccaattgatc agccgcctgc agatgttgtc ggaagatttc gagacggagg
     1621 ttcagggctg gcaggtcgat cgtgtccgcg accagctcag gaccgatcgc tactttcacg
     1681 gcatctatca tccaaaggcc ttccagatcg acggccgcaa atatgtccat ggcctggcgg
     1741 cgctcgcgcg acgcgccgga gcgcggattt tcgaggacac tcctgtcgtc agcgtggatt
     1801 cttcgggcat ccgcaagcgc gtcgtcacgc cgtcggcacg tttgcgcgcg tcccacatcg
     1861 tgctggccgg caacgtccat ctcggcgcgc cgctgcgccg gctgtcggaa accttgctgc
     1921 cggtgtggcg ctttgccggc gtcacggcgc cgcttggcga gcggctggct gaggtgatcg
     1981 cgttcaaggg atcggtcaac gataccgaca at
//
LOCUS       DATLJT010001218         1821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_321537,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001218 DATLJT010000000
VERSION     DATLJT010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321537"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VM910_28075"
     CDS             complement(<1..1242)
                     /locus_tag="VM910_28075"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI66407.1"
                     /translation="MTARPTRREALLQTLLIAGGAAMPNVPLAAAAAVPPAVSRSSEI
                     DRLLRAGLDAGDAPGVVAMAATEKAVLYEGAFGVRCTGAAAKMTTDTVFRIASMVKLV
                     TSVAAMQLVERGKLKLDEPAESIDPTLASPFVLTGFDARGTPQLRPAKKPITLRNLLT
                     HTSGFSYPLWDANVLRYLKAAHGNKNLPRRPLVFDPDSRWAYGGSLDKVSRLVEIASG
                     ESIDRYFDDHIFAPLGMTNTTFVLSVHQRAREASLHVRQADGALKPEPLEKPSQAKVF
                     SGGGGIYSTAPDYLALLQALLNRGSLRGESILRPETVALMSTNQIGDIAAGILKTTNP
                     ALSNDVDFFPGTRLRWGFGHMINLDPVKDGRRAGSLTWAGLYNTYYWIDPASSVAGVI
                     MMQILPFADARALTLYRQFERS"
     gene            complement(1244..>1821)
                     /locus_tag="VM910_28080"
     CDS             complement(1244..>1821)
                     /locus_tag="VM910_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVI66408.1"
                     /translation="KYTDGYLAYAGKDAPKFTADELKVIGSKIDFVGLNIYAPQFYIV
                     ATGRASGFHVLPFPASFPHMNSEWLRIGPETAYWVPRITAKIWKTDNIYITENGTSSE
                     DKVSADGHVYDLDRIMFLRNYLTQLQRAISEGAPVRGYFLWSLMDNFEWIFGFEKRFG
                     LYHVDFETLQRIPKLSVSFYRNVIAKNAVGI"
BASE COUNT          332 a          555 c          597 g          337 t
ORIGIN      
        1 gctgcgctcg aattgccgat agagggtgag cgcgcgcgcg tcggcaaagg gcaggatctg
       61 catcatgatg acgccggcga cgctggaggc cggatcgatc cagtaatagg tgttgtagag
      121 gccggcccac gtcagactgc cggccctgcg tccgtccttg acgggatcga gattgatcat
      181 gtgcccgaac ccccagcgca ggcgggttcc gggaaagaag tcgacgtcgt tcgaaagcgc
      241 ggggtttgtc gtcttgagga tccccgccgc gatgtccccg atctggttgg tggacatgag
      301 cgcgaccgtt tccggccgca ggatgctttc gccgcgcagg cttccccggt tcaaaagcgc
      361 ctgcagcaat gcaagatagt ccggcgccgt ggaatagatg ccgccgccgc ccgaaaacac
      421 cttggcttgc gacggctttt ccagcggttc cggcttcagc gctccatccg cctgtcgcac
      481 atgcaggctt gcctcgcgcg cacgctgatg caccgacagg acgaacgtcg tgttggtcat
      541 tccgagcggc gcgaagatgt gatcgtcgaa atagcggtcg atgctttcgc cgctcgcgat
      601 ctcgaccagc cggctgacct tgtcgaggct gccgccatag gcccatcggg agtccggatc
      661 gaacaccagc ggccggcgcg gcaggttctt gttgccgtgc gcggccttca gatagcgaag
      721 gacgttggca tcccacaggg gatagctgaa gcctgacgtg tgcgtcagca ggttgcgcag
      781 cgtaatcggc ttctttgcgg ggcgcagttg cggcgtgccg cgcgcatcga agccggtaag
      841 aacgaacggc gaggcgagcg tcggatcgat gctctcggcg ggctcatcga gcttgagctt
      901 gccgcgttcg accagttgca tggccgcaac cgacgtcacg agtttcacca tggaggcaat
      961 gcggaagacc gtatcggtcg tcattttcgc cgcagcgccc gtgcagcgga cgccgaaagc
     1021 gccctcatac aggacggcct tttccgtcgc ggccatggcg acgacgcccg gtgcgtcccc
     1081 ggcatcgagg cccgcccgca gcagacgatc gatctcgctg ctgcgcgaga cggcaggagg
     1141 cactgccgcc gcggcggcga gcggcacgtt cggcatcgcg gccccgccgg cgatcagcag
     1201 cgtctgcaac agcgcctcgc gccgggtcgg ccgcgccgtc atgtcagatg cccaccgcat
     1261 ttttggcaat gacgttgcga tagaacgaga cgctgagctt tggaatcctc tgcagcgtct
     1321 cgaaatcgac gtggtagaga ccgaaacgct tttcgaagcc gaagatccat tcgaaattgt
     1381 ccatcagact ccacaggaaa tagccgcgca ccggcgcgcc ttccgaaatc gcgcgctgca
     1441 gttgcgtcaa gtagttgcgc aggaacatga tgcggtcgag atcgtagaca tggccgtcgg
     1501 ccgagacctt gtcctcggag gaggtgccgt tctcggtgat atagatgttg tcggtcttcc
     1561 agatcttcgc cgtgattcgt ggcacccagt aggcggtctc cggcccgatg cgcagccatt
     1621 cggaattcat gtgcgggaac gaagcgggaa acggcaggac gtggaaaccc gacgcgcgcc
     1681 cggtggcgac gatgtagaac tggggcgcat agatgttcag cccgacgaaa tcgatcttcg
     1741 agccgatgac ctttaattcg tcggccgtga acttcggcgc gtccttgccg gcataggcga
     1801 gatagccgtc ggtatacttg c
//
LOCUS       DATLJT010001219         1896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3078683,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001219 DATLJT010000000
VERSION     DATLJT010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078683"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1066)
                     /locus_tag="VM910_28085"
     CDS             complement(62..1066)
                     /locus_tag="VM910_28085"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66409.1"
                     /translation="MTAKMKRREFITLLGGAAAAWPLAARAQQTAAVRRIGVLMPFAA
                     ADAGGHALVASLRRGLKELGWTEDRNIRIEERWAADDTDRIRKYAAELVALAPDVIVG
                     NGTAVLTALRQATRTIPIVFVAVADPVGQGFVASLARPGGAITGFGLEEPSMGGKWLD
                     LLKTIAPHVARIAVIFNPDTAPQSRLFLPSMNAAGLSLTVEWTASPVGTEAEIEQVIG
                     AAGRGQIGGLIVLPDPFTYAHRELIIALAARHRLPAIYPLRSYAESGGLIAYGINRIE
                     LYRHAASYVDRILKGAKMADLPVQMPTKFELVINLKTAKALGLDVPPTLIARADEVIE
                     "
     gene            complement(1063..1362)
                     /locus_tag="VM910_28090"
     CDS             complement(1063..1362)
                     /locus_tag="VM910_28090"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66410.1"
                     /translation="MNTNRVRINTLALGARLPTMHGLREYVEAGGLMSYGPSALDQYR
                     RAADYVDKILRGAKPADIPVEQPTKFDLVINLTTAKALGLQIPDRLLAIADEVIE"
     gene            complement(1359..>1896)
                     /locus_tag="VM910_28095"
     CDS             complement(1359..>1896)
                     /locus_tag="VM910_28095"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66411.1"
                     /translation="FVQRLRELGWIEDRTIAIEVRWAEGRTERFGEIADEFVRLKVDV
                     IVTAGTASVIAAKQATSVIPIVFATAGDPVANHLVASLARPGGNVTGLSGQVVDTASK
                     RLELLRKVLPGLRRLAIMGNVDNPFAVLDMREVQATARMVGLEATTFEIRRAEDIAPA
                     FEAFKGRADALYVPPKRL"
BASE COUNT          351 a          589 c          604 g          352 t
ORIGIN      
        1 ggtccaggca aggatcgagc ctgtcaatcc taacagagcc gcacgatgga cacccggcgc
       61 gtcattcgat cacctcgtcg gcgcgggcga tgagcgtcgg cggcacgtcg aggccaagcg
      121 ccttggcggt cttgaggttg attactaact cgaatttcgt gggcatctgg accggcaagt
      181 cggccatttt agcgcccttg aggatgcgat cgacatagga cgccgcgtgc cggtacagct
      241 cgatacggtt gatgccatag gcgataagtc cgccgctctc agcgtaagaa cgtagagggt
      301 aaatggcagg cagtcggtgg cgagctgcca gtgcaatgat caactcgcga tgggcgtacg
      361 tgaaggggtc cggcagaaca atgaggccac cgatttggcc gcgcccggca gcgcctatca
      421 cctgctcgat ttcggcttcg gtgccgaccg gcgatgcagt ccactctacc gtcaaggaga
      481 ggccggcagc attcatcgaa ggcaggaaca accgggattg gggcgccgta tctgggttaa
      541 agatgactgc gatgcgagca acgtggggcg caatcgtctt caacaggtcc agccactttc
      601 cccccatgct gggttcttcg aggccaaaac cggtaatggc gccgccgggc cgcgccaggc
      661 tggccacaaa gccttgtccg accgggtccg cgactgcaac aaacacaatt ggtatcgtgc
      721 gcgtcgcctg ccgtaacgcg gtcaggaccg ctgtaccatt cccgacgatc acgtccggcg
      781 cgagggcgac taactctgcc gcatatttgc ggatgcggtc agtatcgtcg gcggcccagc
      841 gctcctcgat ccgaatgttg cggtcttcgg tccagcctag ctccttgagc ccccgtcgca
      901 gcgacgcaac gagcgcatgt cctcccgcgt ctgccgcggc aaacggcatc aggactccaa
      961 tccggcgaac agcggcggtt tgctgtgcgc gcgccgcaag cggccacgct gccgccgcgc
     1021 cgccgagcag cgtgatgaac tcgcgtcgtt tcatcttcgc ggtcattcga tgacctcgtc
     1081 ggcgatagcg agcagccggt ctggaatttg gaggccgagc gccttcgcgg tcgtcagatt
     1141 gatgacgaga tcgaatttgg tcggttgctc gaccgggatg tcggccggct tcgctccgcg
     1201 cagaatctta tcgacatagt cggcggcgcg tcgatactgg tccagggcgc ttggtccata
     1261 ggacatcaga cctcccgctt cgacgtactc ccgcaggccg tgcatcgttg gcagtcgcgc
     1321 ccccagcgcc aaggtgttga tgcgaacgcg gttggtgttc acaagcgctt cggggggacg
     1381 taaagtgcgt ccgcgcggcc cttgaacgcc tcgaaggcgg gcgcgatatc ttcagctcgc
     1441 cggatttcga aggtagtggc ttcgaggccg accatgcggg ccgtcgcttg aacctcgcgc
     1501 atgtccagca cggcgaaggg attgtcgacg ttgcccatga tcgccaaccg gcgcagaccg
     1561 gggagaactt tccgcaaaag ttcgaggcgc ttggaggcag tgtcgactac ctggcctgac
     1621 agcccggtga cattgccgcc aggccgcgcc aggctggcga cgagatggtt cgcgaccggg
     1681 tcacccgctg tcgcgaacac gatcgggatg actgatgtcg cctgctttgc tgcaatgacg
     1741 gatgcggttc ccgccgtgac aatgacatcg accttgagcc gaacgaactc atcggcgatc
     1801 tcgccgaagc gctcggtgcg tccctcagcc cagcgaacct cgattgcgat ggtgcgatcc
     1861 tcgatccaac cgagttcgcg cagtcgctgc acaaaa
//
LOCUS       DATLJT010001220         4168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1929706,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001220 DATLJT010000000
VERSION     DATLJT010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929706"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="sucA"
                     /locus_tag="VM910_28100"
     CDS             complement(<1..656)
                     /gene="sucA"
                     /locus_tag="VM910_28100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609293.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HVI66412.1"
                     /translation="MSRQDANAALARTSFLYGGNADYIENLYARYGTDPTAVDAQWQA
                     FFQSLKDDRADVMTNARGPSWQRPYQPARSELISALDGDWREVERALGDKVKARAQAA
                     GVDFSAAEVQQTTRDSIHALMLIRAYRARGHFYANLDPLGLEPHHAEEDLDPRSYGFT
                     ETDYDRKIFLDRVLGLEFGTLREILAILRRTYCQTLGVEFMHISDPAQKSWIQARIEG
                     P"
     gene            complement(922..1683)
                     /locus_tag="VM910_28105"
     CDS             complement(922..1683)
                     /locus_tag="VM910_28105"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI66413.1"
                     /translation="MKKLFLATTAFVVLAAASAGAADMYARPAYTPPPAPAPVYTWTG
                     MYWGANVGYSWGQSKNDRTGSLIGLNATVTESQDVDGAIGGVQTGYNYQFGTWVFGLE
                     TDIQASGQKGGSTIPLLALAPSALTTDHKLQWFGTTRPRLGFLATPNLLLYGTAGVAY
                     GQTKDTYTVNTVGVGTAATASINDVRAGWTAGAGIEGAFGGGWSAKVEYLYMDLGKLE
                     QTVTVPPAALTATFNSRLTDNIVRVGLNYKWGGGY"
     gene            complement(1935..2819)
                     /gene="sucD"
                     /locus_tag="VM910_28110"
     CDS             complement(1935..2819)
                     /gene="sucD"
                     /locus_tag="VM910_28110"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HVI66414.1"
                     /translation="MSILIDKNTKVICQGFTGKNGTFHSEQAIAYGTKMVGGTSPGKG
                     GTSHLGLPVFNTVAEAKEKTDCDASVIYVPPAGAADAICEAIQAEISLIVCITEGIPV
                     LDMVKVKRALSGGKSRLIGPNCPGVMTAGECKIGIMPGNIFTPGKVGIVSRSGTLTYE
                     AVFQTTRVGLGQTTAVGIGGDPVKGTEFIDVLAMFLADKATEAIVMIGEIGGSGEEDA
                     AQFLADEAKRGRKKPMVGFIAGRTAPPGRRMGHAGAIISGGRGDAESKIAAMEAAGIK
                     VSPSPARLGNTLAEQLRR"
     gene            complement(2926..4092)
                     /gene="sucC"
                     /locus_tag="VM910_28115"
     CDS             complement(2926..4092)
                     /gene="sucC"
                     /locus_tag="VM910_28115"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HVI66415.1"
                     /translation="MNIHEYQAKAVLREFGVPVPRGHAAFSVEEAVKAASDLGSPLLV
                     VKAQIHAGGRGKAGGVKLVKSREDVRKEAERLLGAVLVTHQTGPHGKQVHRLYIEEGS
                     AIDREFYLSALVDRESSRIAFVVSTAGGMEIEEVARTRPEQIVTFAVDPATGIMPHHG
                     RRVAQALGLHDDLAKQAEKLVTQLYAAFTAKDMSLLEINPLVVTRGKELVCLDAKIGF
                     DDNALYRHPDIVALRDLTEEDEKEIEASKYDLNYVALDGTIGCMVNGAGLAMATMDII
                     KLYGQAPANFLDVGGGASKEKVAAAFKIITADPKVQGILVNIFGGIMKCDVIAEGVVA
                     AVKEVGLRVPLVVRLEGTNVELGKDIIAKSNLNVTSADDLDDAAQKIVKAVKGG"
BASE COUNT          721 a         1340 c         1288 g          819 t
ORIGIN      
        1 ggaccctcga tgcgcgcctg gatccaactc ttctgcgcgg ggtcggagat atgcatgaac
       61 tcgacgccga gcgtctggca ataggtgcgg cggaggatcg cgagaatttc gcgcaaggtg
      121 ccgaattcga ggccgagcac gcggtcgaga aagatcttgc ggtcgtaatc cgtttccgtg
      181 aagccgtagg agcgcggatc aagatcctcc tcggcatgat gtggctcgag gccgagcggg
      241 tcgagattgg cgtagaagtg cccgcgcgcg cggtaggcgc ggatcagcat caaggcgtgg
      301 atcgaatcgc gcgtcgtctg ctggacctcg gcagcggaga agtcgacgcc cgcggcctgc
      361 gcccgcgcct tgaccttgtc gccgagcgcg cgctccacct cgcgccaatc gccgtcgagc
      421 gcggagatga gttcgctgcg cgcaggctgg taggggcgct gccatgacgg cccgcgcgca
      481 ttcgtcatga catcggcgcg gtcgtccttc aggctctgga agaacgcctg ccattgggca
      541 tcgaccgcgg tggggtcggt cccgtagcgg gcgtagaggt tctcgatgta gtcggcattg
      601 ccaccgtaaa ggaacgatgt gcgggcgagg gcggcgttgg cgtcctgacg agacatgctc
      661 aggtcctgta acgttcagct tttggcgggc ggcggatcgg ccgccagcag ggatccggcg
      721 gcgagtctac ttattacatc atttatgctg cagttgcgtt gtcgcaatcc acaagaactg
      781 aactctcgtt cacccgacta gcagacgagg atgaccgcca attaacggct gaaggacggt
      841 ctcgccctgt gagcgcttcc ctaacgaaaa agggccgctt gcgggtgcaa gcggccctcg
      901 gccctgaact caaatcagcg attagtatcc cccgccccat ttgtagttca ggccaacgcg
      961 cacgatgtta tcggtcaggc ggctgttgaa ggtcgcggtc agcgcggcag gcggtacggt
     1021 aaccgtctgc tccaacttgc cgaggtccat atagaggtat tcgaccttcg cgctccagcc
     1081 gccgccgaat gcgccttcga tgccagcgcc ggcagtccag ccggccctca cgtcattaat
     1141 gcttgccgtc gcagcggtcc cgacgccaac tgtgttgacg gtgtaagtgt ccttggtctg
     1201 cccgtaagcc acaccagccg ttccgtacag gaggagattg ggagtggcca gaaagccaag
     1261 gcgcggccgc gtggtgccga accactggag cttgtgatcg gtggtcaatg cggagggagc
     1321 aagcgccaag agcggaatcg tcgagccgcc tttctgaccg gaggcctgaa tgtcggtttc
     1381 caacccgaac acccacgttc caaactgata gttgtagccg gtttggacgc cgccgatcgc
     1441 gccgtcgacg tcttgggatt ccgtcacagt ggcgttgagg ccgataagac taccagtacg
     1501 gtcgtttttc gattggcccc agctgtagcc gacgttggcg ccccaataca tgcccgtcca
     1561 ggtgtagacc ggagcgggcg ccggaggcgg cgtgtaagcc ggccgcgcgt acatgtctgc
     1621 ggcccccgcg gaagccgccg ccagcacaac gaaagcggtc gtagcaagga agagtttttt
     1681 catcacgaat cccccatccg atgttcggtt cggcaggccg ctgccctaac gtttccccgg
     1741 caacaacttg aaccccttac gccggcgctc ggataacgcc tgcacgtgtc cttttttagc
     1801 ctggccgttt gaaataagct gtgacctctc cgccacagtc gcgggcaaaa gcgactttcg
     1861 gatgcacctg ggtaggtcgt cccagtaccc tttttctccc gcacaccgcc atgccccggg
     1921 agcggcttgc cattttacct gcgcagctgc tcggccaagg tgttgcccaa acgcgccggc
     1981 gacggcgaga ccttaatgcc ggcggcttcc atggccgcaa tcttgctttc cgcgtcgccc
     2041 ctgccgccgg agatgatggc gcccgcgtgg cccatgcgtc ggcccggcgg tgcggtacgg
     2101 ccggcaatga accccaccat cggcttcttg cgtccgcgtt tggcttcgtc ggcgaggaat
     2161 tgggccgcat cctcctcgcc cgagccgccg atctcgccga tcatcacaat cgcctcggtc
     2221 gccttgtcgg ccaagaacat cgccagcaca tcgatgaact cggtgccctt caccgggtcg
     2281 ccgccgatgc cgaccgccgt ggtctggccg agccccacgc gcgtggtctg aaagaccgcc
     2341 tcataggtga gcgtgcccga gcgcgacacg atgccgacct tgccgggcgt aaaaatgttg
     2401 cccggcatga tgccgatctt gcattcgccc gcggtcatca cgcccggaca gttcggcccg
     2461 atcagccgcg acttcccgcc cgacaatgcg cgcttgacct tcaccatatc gagcaccgga
     2521 atgccttcgg tgatgcagac gatgagggaa atttccgcct ggatcgcttc gcaaatcgcg
     2581 tccgcagcgc cagccggcgg cacgtagatc acgctcgcgt cgcaatcggt cttctccttc
     2641 gcctccgcca ccgtgttgaa gacgggcagg ccgagatggc tggtgccgcc tttgcccggc
     2701 gacgtgcccc cgaccatctt ggtgccgtac gcgatcgcct gctcggaatg gaacgtgccg
     2761 ttcttgccag tgaaaccctg gcagatgacc ttggtgttct tatcgatgag aatggacatg
     2821 cgtgcgttca gcgatgcggc gagcttggcg catctccctc tgacagcagc ctgaaagagg
     2881 caaagcgagc gacatggccg cgcgtttttc gatctggcgt catcgtcagc cccccttcac
     2941 cgccttgacg atcttttgcg cggcgtcatc gagatcgtcc gcggaggtga cgttgaggtt
     3001 cgatttcgcg atgatgtctt tgccaagctc gacgttggtg ccttcgaggc gcaccactag
     3061 cggcacccgc aaacccacct ccttcactgc tgcgaccacg ccttcggcga tgacatcgca
     3121 tttcatgatg ccgccgaaaa tgttgaccag gatgccctgc accttgggat cggcggtgat
     3181 gatcttgaag gcggccgcga ccttctcctt gctggcgccg ccgccgacgt cgagaaaatt
     3241 cgccggcgct tggccgtaaa gcttgatgat gtccatggtc gccatggcga ggcctgcgcc
     3301 gttgaccatg cagccgatgg tgccatcgag cgcgacgtaa ttgaggtcgt atttcgacgc
     3361 ctcgatctcc ttctcgtcct cctcggtgag atcgcgcaac gccactatgt cgggatggcg
     3421 gtagagcgcg ttgtcgtcga agccgatctt ggcgtcgagg cagaccagct ctttgcctct
     3481 ggtgacgaca agcggattga tctcgagcag actcatgtcc ttggcggtga aggcggcata
     3541 aagctgcgtc accagctttt ccgcctgctt ggcaagatcg tcatgcagcc caagcgcctg
     3601 cgccacccgc cgtccatgat gcggcatgat cccggtcgcg ggatcgacgg cgaaggtcac
     3661 gatctgttcg ggccgcgtgc gcgcaacctc ctcgatctcc attccgcccg cggtcgaaac
     3721 cacgaaggcg atgcgcgagc tttcgcggtc gacgagtgcc gagagataga attcgcggtc
     3781 gatggccgag ccttcctcga tatagaggcg gtggacctgc ttgccgtgcg ggccggtctg
     3841 atgggtgacg agcaccgcgc cgagcaggcg ctcggcctcc ttgcgcacgt cctccctcga
     3901 cttcaccagc ttgacgccac cggccttgcc gcgcccgccg gcgtggatct gcgccttgac
     3961 gacaagcaat ggcgaaccga gatcgctcgc agccttgacc gcctcctcga ccgaaaaggc
     4021 cgcatgtccg cgcggcaccg gcacgccgaa ttcccgcagc accgccttgg cttgatattc
     4081 gtggatgttc attcggacct ttacgtatcg cagatgcagt gcacgcggac cagtttacgc
     4141 agtctcggca agcttggctg ctctgcgc
//
LOCUS       DATLJT010001221         5481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_778752,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001221 DATLJT010000000
VERSION     DATLJT010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778752"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..814)
                     /locus_tag="VM910_28120"
                     /pseudo
     CDS             complement(1..814)
                     /locus_tag="VM910_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511202.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(851..1138)
                     /locus_tag="VM910_28125"
     CDS             complement(851..1138)
                     /locus_tag="VM910_28125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66416.1"
                     /translation="MPIEKVLAFAEGQLPERERAYVEDTFALPIGEALATALPYRHRK
                     CRIYEQQIVLDLQGQIIVCCGVYDYKSSGGLGSFLEMTPKELMKAKEIPEV"
     gene            complement(1371..1891)
                     /locus_tag="VM910_28130"
                     /pseudo
     CDS             complement(1371..1891)
                     /locus_tag="VM910_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
     gene            2134..2889
                     /locus_tag="VM910_28135"
     CDS             2134..2889
                     /locus_tag="VM910_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HVI66417.1"
                     /translation="MSVMDDRAAFEEKKRRQALALAQDQRLFADAIDMLHAADKYDYS
                     YLWSWMGVPIIQLPADIITTQEVIWATKPDVIIETGVARGGSVLFMASILELIGKGKV
                     IGVDIDIRAHNRDTVDHHPMAKRVILIEGPSTDPTTLAKVRAEIPAASAVMVVLDSDH
                     SRDHVLGELRSYGPLVTQGCYLVAADTVLGHLDPDQTPRNRSKVCFRGNEPLSALEAY
                     LKETDRFEIDPVINGKLILSSSPGGYLRCRKEA"
     gene            complement(3598..4266)
                     /locus_tag="VM910_28140"
     CDS             complement(3598..4266)
                     /locus_tag="VM910_28140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HVI66418.1"
                     /translation="MPPGLSISDPAIIWGGTGQARVIRPILEAMGFRLVMVFDQHLVT
                     PFADVPVGVGLPDFEQWRAENGGDCAFAVCIGGTLGKDRLEVHNRLLRAGLTPTIAVH
                     HRAWVAGSVSLAPGCQVLAMAAVAEGTTLGTACIVNTGAQVDHDCTLGHAVHVMPGAV
                     IAGEVVIEDFASIGSNATVLPRLKIAKGAIVGAGAVVTKDVPPGITVVGNPARPHRRP
                     NHNG"
     gene            complement(4454..4720)
                     /locus_tag="VM910_28145"
     CDS             complement(4454..4720)
                     /locus_tag="VM910_28145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66419.1"
                     /translation="MQAIFPSAQSAESIILPLDYCRARNLEIMKKPVNIVPIWSTKLG
                     RYVETIWPSINEIETTASRTKEWAGMDPPKWGEATVSIDSSAGR"
     gene            complement(4805..4996)
                     /locus_tag="VM910_28150"
     CDS             complement(4805..4996)
                     /locus_tag="VM910_28150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66420.1"
                     /translation="MNELLGQMALGVEAYLKSEGLRITSFGVGWSASCTDKDGNAVEI
                     NPMDMRRPKPWSKKRKRRS"
     gene            complement(5058..>5481)
                     /locus_tag="VM910_28155"
     CDS             complement(5058..>5481)
                     /locus_tag="VM910_28155"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVI66421.1"
                     /translation="ALGLAQLERLPELIERKRAIFDWYRRALGNVPGVTLNSEAPDVV
                     NVYWMVTAIVDPKLGWTKEKLVPALREQNVDCRPFFYPLSMLPVYESFPSAAAARARN
                     SAAYAVAPYGVNLPSALLLTQDQVELVVAKLKMLLQGV"
BASE COUNT         1080 a         1586 c         1687 g         1128 t
ORIGIN      
        1 ggaggcggcg aaagttgtag ccggcggcgg cgagcacggc attgatggcg tcgctgcttg
       61 cgtgtgcgag gtaattgcga cccatgcggg gatgttcctt gagatggcgg atgacgggct
      121 caatggcggc acgccctttg aactcgcgct tgatctgcgg cgtgacgcgg cgcttctggc
      181 cgctcgtgta gaccttgaat ttgtgttcgg gcggagcgtt gtggccgcga tagccggcat
      241 cggcgacgca gcaatcgagt acgttgccga tgagcgcttg catctgtgga atgacggtgg
      301 cgagcgtgtg cccgtcataa ggattgcccg gcaacgctgc gacgtgggag acaaactgcc
      361 cgccggcgga atgcttcacc gtggtagcga cgctcacctt gacgccgaac tcgtagggcc
      421 ggtgcgcctt gcccttgccg atgcactcga cctctggcgc gtgcagggaa tagaccttgc
      481 ggccgcgctg gcggcgctcc tattccagca cgcgccgggc caggaacagg ggccgtacga
      541 atgcttcctg caaatcttcg ttccagacga ttcgccgctc gatgtcgcgg atgacacggc
      601 cgagataggt cttgagcttg cgtaagctgc ggttggcgcg cttgaattga tgggcatggg
      661 cgtagcgctg gtgcttaatc agagccagct tgccgacccg cgcataggat tggcgcaggc
      721 tcactccgag cttcttcgtc agtcgcacca gccgctcgcg cgcccggttg agcagtttgg
      781 cgtcggtcgg aaacatcacc gccttcggct gcacccgccc cgaggaactc cacgatttgt
      841 catacccatt tcatacttcg ggaatttcct tggccttcat taattctttt ggtgtcattt
      901 caagaaatga tcctaaaccg ccgctagact tatagtcata gacgccgcaa cacacgatga
      961 tctgtccctg gaggtcgaga acaatctgtt gctcatatat gcggcacttg cggtggcgat
     1021 aggggagggc ggttgcgagg gcttcaccaa tcggcaatgc aaatgtgtcc tcaacgtagg
     1081 ctcgttcacg ttccggaagc tggccttctg caaacgcgag gaccttttcg atgggcatgt
     1141 agtatgccca attctcaagc caactaaacc caagggaggt cgcgtattct ttgcattcgg
     1201 gcaagttcgc caagattgtg gtggtacttg tgaaaataca cttgaacatc agtggttttg
     1261 ttgccaatgc aagtcgcctg gctgataccc gccttacggc agatcaccgg cacccaatca
     1321 gaactctgct cgaataaacg ccttcctgcg tgtccgaatc tcgaagtctt tcataggtca
     1381 tgcagtcttg gcagcgcccg gtcacgcgcc gaaatcacgc gctgtgtggc cttcggccag
     1441 acgatggcga gatcgggatc atcccagcgg acgccgcgcg cggcttccga gcgatggggt
     1501 tccgagatat ggtagacgac ttcggcgtca tcggtcaaag tctgaaaacc gtgggcaaat
     1561 cctgctggga tgtatatcga cgcccgattg tcggcggtca actcgatcgc gacccatttg
     1621 gcgcgtgtgg gggatcccgc acgtcgaata tcgcccctcg cgtgcaacgg acgagcttga
     1681 tctcttcata cggggcagct tggaagtgga ggccgcgcac cgtgtcgcga tggtgattga
     1741 acgagatgct ccactgcggg taatgtgtgc aaagcccccg ctccatgaac agatcgctgc
     1801 agaacgaacg cgcaaaaaaa ccgcgatcat cttccagctt ttcgagttgg atgaccaacg
     1861 gtccagggat gttcagtggg gaaaatagca tcgcaggcga tcgaatctcc ggggagttgc
     1921 cgaagtggac tgcagcgccg aggcgggcgg gaaagctacc agaacaatcg aatttttccc
     1981 acgggtgccg cgctcttaga tcacaatggt ccaattgggg gcgtctaggc gaggcacgtc
     2041 ggtcgattgc acccgatcgg ctggcttgca aggcgttgga atgctaaaca cgaccatgct
     2101 tcccgaaaag tcgacaaatc cccggagcga gtcttgagtg tcatggacga tcgcgcagca
     2161 tttgaagaaa aaaagcgccg gcaggcattg gctcttgcac aggatcagcg cctcttcgct
     2221 gacgcaatcg atatgctcca tgccgccgac aagtacgact actcctatct ttggagctgg
     2281 atgggcgtcc ccattatcca acttcctgcc gacataatca cgacgcaaga ggtgatatgg
     2341 gcaacaaaac ctgacgtgat tatcgagaca ggcgtggcac gcggcggctc agtgctgttc
     2401 atggcatcga ttctggagct catcgggaag ggcaaggtga tcggtgttga catagacatt
     2461 cgtgcccaca accgggatac ggtcgaccac catcccatgg ccaagcgggt gatcctgatc
     2521 gaggggcctt cgaccgatcc caccacactc gcgaaagttc gagcggagat cccggcggca
     2581 tctgcggtga tggtcgtcct cgattccgac cacagtcgcg accacgtgct cggcgagctg
     2641 cgaagctatg gtcctctggt aacgcagggg tgctatctcg tagccgccga caccgtctta
     2701 ggccatcttg atcccgacca gacacctcgt aatcgctcaa aggtgtgttt cagaggaaac
     2761 gagccgctga gcgcgctaga ggcgtacctc aaagagactg atcggtttga gatcgacccg
     2821 gtaatcaatg gaaagctgat tctgtcgagt tcacccggag gctacttgcg gtgccgaaaa
     2881 gaagcatagc tctttccact cggaaaacgt acgtgatcac gctcgatgcc acaggcgcgg
     2941 ttcgagcgga ggtctcggcg ggaaaagaga cccatcgatt tgaggtcgac tcagtcatta
     3001 atggcgcccg ctcgatatct gcgctgctgg tagaactaaa aggctgcgat gggcgtggaa
     3061 agagctcctg cctcaaagtt gctgcgccac tgcacgtcat aaggctacag accaaggggt
     3121 gtcacgatta ctgttcttat cgctgggatc ggcggcgctt ctctcgggac cgagattgcg
     3181 aagtcacttc gcttggcctc gagtatcgct tgatcgctat atcgatcacc tggtcgaaat
     3241 ttcgcgcgaa gagagcatct gcaaacgatt gttgcagaag agccaatgag gctcattaca
     3301 gccgcggcat gtcggttttc tcaagaagga attcggctgg ccatgaactc gcaggacgtc
     3361 gtgaaacgct gtttcatcga agtgcaaacg tctcggaatt gcgattccac gaacggttgc
     3421 ggcaaccgaa cccacaatga tcgaccgggt gccattcccc gtgcataatt aaaccgtccg
     3481 tcgaaagcgg gggtagctcc ctggtgctct ttgcgcgcaa ccgggaggaa gcaaacctct
     3541 atgctaccta tctaaaagtt gccggcaagc gctcggctac gtttcggtga acgtggttta
     3601 cccgttgtgg ttcgggcggc ggtgaggtcg ggcggggtta ccgacgacgg tgatgcctgg
     3661 cggaacgtct ttggtgacga cggcgccggc gccaacgatt gcgcccttgg cgatcttgag
     3721 ccgcggcagc acggttgcgt tggagccgat cgacgcaaaa tcctcgatca ccacttcgcc
     3781 ggcgatcacc gcgcctggca tgacgtggac ggcgtggccg agggtgcaat cgtggtcgac
     3841 ctgggcgccg gtgttgacga tgcaagcggt gccgagtgtg gtgccctcgg cgacggcggc
     3901 catagcgagg acctggcaac ctggtgcgag gcttacgcta cctgcgaccc aagcgcggtg
     3961 gtggacggcg atcgtcggcg tgaggccggc gcgcagcagg cgattgtgga cctcgaggcg
     4021 gtccttgccc agcgttccgc cgatgcaaac cgcaaacgca cagtcgccgc cgttctcggc
     4081 gcgccattgc tcgaagtcag ggagaccgac gccgaccggc acgtccgcga acggcgtgac
     4141 taggtgctga tcgaatacca tgacgaggcg gaagcccatg gcctcgagga tggggcggat
     4201 gacgcgggcc tggccggttc cgccccagat gatggcgggg tccgatatgc tgagtcctgg
     4261 tggcacggtc cccaatccgc tcgaacacaa tttgctctgc ccggatgttt tgggtcgcat
     4321 acggcctgcc gtccgtcgat agacccgaga cttgcgaggt tcgcatggac ttgagttggc
     4381 ttctacgtcc cgcagggaag ggcgccgcca gctgcgggac acattcatcc gctttgaccg
     4441 atattctgca ccgctagcgg ccggcggatg agtcaattga tacggtggcc tcgccccatt
     4501 tcggcgggtc catcccggcc cattctttcg tgcgcgaggc cgtcgtttcg atctcattaa
     4561 tcgacggcca gatcgtttcg acgtagcgcc ctagcttggt gctccagatc ggcacgatgt
     4621 tcaccggctt tttcatgatc tcgagattgc gggcgcggca gtagtcgagc ggcaggatga
     4681 tgctctcggc gctttgcgcg ctgggaaaga tcgcttgcac cagcacgcgc cattggccgg
     4741 catcgacggg gcgagccggc cggcatcggc aagcgcgggc cggccttagt tacttcgttc
     4801 gccatcaact cctcctcttg cgtttcttcg accacggttt cggccgtcgc atgtccatcg
     4861 ggttaatctc gaccgcgttg ccgtccttgt ccgtgcagct cgcactccag ccaacgccga
     4921 agctcgtgat gcgcaagcct tcgcttttga gataggcctc gaccccgagc gccatctgcc
     4981 ccagcaattc gttcaacgtt caagccacca gcgcgacagg cgcgatgtcc aatggtcgtt
     5041 gcatttgaga cggggcttta cacgccctgc agcaacatct tcagtttggc gacgacgagc
     5101 tccacctgat cttgtgtgag caacaatgcg ctcggcaggt tgaccccgta gggcgcaacc
     5161 gcgtaggcgg ccgagttgcg ggcacgcgcc gcggcggcgc tgggaaatga ttcgtaaacc
     5221 ggcagcatgc tcagcgggta gaagaaaggc cggcaatcga cgttctgctc gcgcaaggcc
     5281 ggaacgagct tctccttggt ccagccgagc ttcggatcga cgatggccgt caccatccag
     5341 tagacattga ccacatccgg cgcttcgctg ttgagcgtga cccccggaac gtttccaagc
     5401 gcacggcgat accaatcgaa gatcgcgcgc ttgcgctcga tcagctcggg caagcgctcg
     5461 agctgcgcga ggccgagcgc c
//
LOCUS       DATLJT010001222         4269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1241147,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001222 DATLJT010000000
VERSION     DATLJT010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241147"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VM910_28160"
     CDS             complement(<1..407)
                     /locus_tag="VM910_28160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66422.1"
                     /translation="MSENTPPILSNDEQVAPNPFDPERLRIDLSFAEGIGVKKALVTV
                     PVRKPNGQDFIRAHPDPSFRLPVATIELKDDRETYLVIPDIARDIPGEYVMSTMHTCI
                     NRAGVVFLWPVRLPSPDGRQLAWHHSAQVAAEMA"
     gene            complement(483..1055)
                     /locus_tag="VM910_28165"
     CDS             complement(483..1055)
                     /locus_tag="VM910_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI66423.1"
                     /translation="MLDHSSQVQRHPQAERGLDLYETPAVAVEALLRAEKLAARIWEP
                     AAGRGAIVRVLREHGHHVVASDVFDYGGLDFVGDFLAQEQMPVGCDAIVTNPPFMIAE
                     PFVERALELAPLVIMLLRLAFLESERRCHILEGRGLARVHVFRKRLPMMHRDRWAGKK
                     ANSGMAFAWMVWLRGYNDGPTIINRISWER"
     gene            complement(1048..1242)
                     /locus_tag="VM910_28170"
     CDS             complement(1048..1242)
                     /locus_tag="VM910_28170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66424.1"
                     /translation="MADTEQPFIDHWPLSQFDEARSKFPCLADIEAARIKLARRFNSK
                     PLPKATEVDLAWQEHRRRHA"
     gene            complement(1246..1683)
                     /locus_tag="VM910_28175"
     CDS             complement(1246..1683)
                     /locus_tag="VM910_28175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66425.1"
                     /translation="MTGTASALLPQGACGTTAAEDDGLGIPHILTISREARAAAWNRY
                     QPPPPPPIVPREVEVARAAEKKRRAYARIAKLKAKKATATATAMAVTMATTTTITAVV
                     TRIPAGILADERWPGMYRVKLGRSQLSDMLNLTRARDVLASRG"
     gene            complement(1749..1952)
                     /locus_tag="VM910_28180"
     CDS             complement(1749..1952)
                     /locus_tag="VM910_28180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66426.1"
                     /translation="MSAKLKFHPLADIFPLMEGAEFEELVADIKASGCGPTTVRLSDK
                     RMGVTVAAHRAWLASRERASPNL"
     gene            complement(1949..2140)
                     /locus_tag="VM910_28185"
     CDS             complement(1949..2140)
                     /locus_tag="VM910_28185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI66427.1"
                     /translation="MSEETPLTISVPEAGKRYFGLCRNASYEAAARGEIPVVRIGKLL
                     RVPVRALERMLDRAGEPTP"
     gene            2297..2593
                     /locus_tag="VM910_28190"
     CDS             2297..2593
                     /locus_tag="VM910_28190"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HVI66428.1"
                     /translation="MARKPTDTVKLQLRLPEGLRRRLAYLATRSGRSMNNEIVHLLTR
                     AIAFVGPSGEIPGENKGELGNLEKKGAELVLNIDGKLYRFETKDTVHILKKDKP"
     gene            2590..3744
                     /locus_tag="VM910_28195"
     CDS             2590..3744
                     /locus_tag="VM910_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66429.1"
                     /translation="MKGHIRERSPGHWAIVIDVRDSQTGKRKRRWHSFAGTKRQAQVE
                     AARLISELQKGAAVEPNRITLAQFLDQWLEYIEPQVAPRTHERYGEIVRTYLAPALGT
                     TLLTKMQAMTISAAYAGMLSGGRRKGNGGLSPRSVHHCHRILSQALQQAVRWRMLSRN
                     PCNDVDPPRIERRALKVWDVATMAQALECARSLRIFMPMLLAGMLGLRRGEIAALRWR
                     HVDLDRAQVAIVESFEQTRLGVRVKAPKSGRGRKVALPTRVVAELRTWRTKQAQELLR
                     LGIRLNDDCFVCAGEDGQPIQPQSLTHAWHRFLARTNLPRLRFHDLRHSHATHMLASG
                     VHPKIASERLGHATVGLTLDTYSHVIPGMQEDAVARIDAAFAGTVGKPIG"
     gene            3788..3864
                     /locus_tag="VM910_28200"
     tRNA            3788..3864
                     /locus_tag="VM910_28200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3822..3824,aa:Arg,seq:cct)
     gene            complement(3885..>4269)
                     /locus_tag="VM910_28205"
     CDS             complement(3885..>4269)
                     /locus_tag="VM910_28205"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66430.1"
                     /translation="RRHRTEQAAEHLRLGLKLNLVFAHPDGSPLDPDTLTKSFDKLIR
                     ATGVRRITFHGLRHTHISHQLMDGVHIKIVSERAGHASVSTTLAVYAAFIPNMQADAA
                     AGVDAWLREALGGTPVAIAGFGKRE"
BASE COUNT          784 a         1276 c         1371 g          838 t
ORIGIN      
        1 gccatctcgg ccgccacctg cgccgaatgg tgccatgcga gctggcgccc atccgggctc
       61 ggtaaccgca ccggccacaa gaagacgacc cctgctcggt tgatgcacgt gtgcatcgtc
      121 gacatgacgt attccccggg aatatcgcgg gcgatgtccg ggataaccaa gtacgtttcc
      181 cggtcgtctt tgagctcgat ggtcgcgacc ggcagccgaa acgatggatc cggatgcgct
      241 cgaatgaagt cctgcccatt cggtttccgg acggggaccg tgacgagcgc cttcttgacg
      301 ccgatgccct ccgcgaagct gaggtcgatg cgcaaccgct cggggtcgaa tgggttgggt
      361 gcgacttgct cgtcgttcga caagataggt ggagtgtttt ccgacatgct ggggtctcct
      421 cttctgtcgg ggtacggcgg aatgcgcgcc gcgcgccttg ccacatcgtg gcgagatttc
      481 gttcagcgct cccatgagat gcggttgatg atggtcgggc cgtcgttgta gccgcgcagc
      541 cacaccatcc acgcgaacgc cattccgctg ttggccttct ttccggccca ccggtcgcgg
      601 tgcatcatcg gtaggcgctt gcggaacacg tggacgcgcg cgagcccacg gccttccaag
      661 atgtggcagc ggcgctcgct ctcgaggaac gcaagccgta acaacatgat cacgagcgga
      721 gcgagctcga gcgcacgctc gacgaacggc tctgcaatca tgaacggcgg gttggtgacg
      781 atggcatcac agccgaccgg catctgctcc tgcgccagga agtcaccgac gaagtctaat
      841 ccgccatagt cgaacacgtc ggacgcgacg acgtggtggc cgtgctcgcg cagtacacga
      901 acgatggcgc cgcgaccggc ggcgggctcc cagattcgcg cggctagctt ctcggcgcgc
      961 aacagtgcct cgaccgcaac ggcgggagtt tcgtacaagt cgagaccgcg ctcggcctgc
     1021 gggtgccgtt ggacctgcga ggaatggtca agcatggcga cgtcggtgct cttgccacgc
     1081 gaggtccacc tcggttgcct tgggcagcgg cttcgaattg aatcgtcgcg ccaacttgat
     1141 gcgcgcggct tcgatgtcgg ccaggcacgg aaatttggag cgcgcctcgt cgaattggga
     1201 tagcggccag tggtcgatga acggctgctc ggtgtcggcc atatttcacc ccctagatgc
     1261 gagcacgtcg cgtgcgcgcg taaggttcag catgtcgctg agctggctgc gaccgagctt
     1321 cacgcgatac atccccggcc agcgttcgtc ggcgagaatg ccggcaggga tcctggtcac
     1381 gacggcggtg atggtggtgg tggtcgccat ggtgaccgcc atcgcggtgg cggtggccgt
     1441 ggccttcttc gccttcaact tggcgatgcg cgcgtaggcg cggcgcttct tctcagccgc
     1501 acgggcgacc tcgacctcgc gcggtacgat gggtggtggt ggtggtggtt ggtagcggtt
     1561 ccaagccgcc gcccgcgctt ccctcgagat ggttaggatg tgcgggatcc cgaggccgtc
     1621 gtcttctgcg gccgtggtgc cgcacgcccc ttgcgggagg agcgcagatg cagtaccggt
     1681 cattgtgtct aacgtgcctt tctaaccccc ggcagggtcc agcctgctgg gggcttccgt
     1741 tttccaggtc agagatttgg ggaggcgcgc tcgcgcgatg cgagccaagc tcggtgcgcg
     1801 gcgacggtga cgcccatgcg cttgtcgctg aggcgcacag tggtcgggcc gcagccgctc
     1861 gccttgatgt cggcgaccaa ctcctcgaac tcggcaccct ccatcaaggg gaagatgtcg
     1921 gcgagggggt gaaacttgag tttggcgctc atggcgtcgg ctcgccagcg cggtcgagca
     1981 tccgctccag cgcccgcacc gggacgcgca acagcttgcc aattctgacg acggggattt
     2041 ccccgcgcgc tgccgcctcg taggaggcgt ttcgacaaag tccgaaatac cgcttcccag
     2101 cctcaggaac cgagatcgtt agcggggttt cctcgctcat ggggtcaata tcctattgtc
     2161 cgaacccgta ggtgcattgt gcctctgtag gtgcgttgtg cccctatgtg gtcctacgcc
     2221 acaagtttgt caatagaggc attgtgcacc taccggagat gccatatagt cccgctcggt
     2281 cataaggggg agaacgatgg ccaggaaacc gactgacacc gtcaaactac agctgagact
     2341 tccggagggc ctgcgccggc gcctagcgta cttggcgacc cgaagcgggc gttcgatgaa
     2401 taacgaaatc gttcacctgc tgacgcgggc catcgccttt gtgggtcctt cgggcgagat
     2461 tccaggcgaa aataaaggcg agttggggaa tctcgaaaaa aagggcgccg agcttgtgct
     2521 gaacattgat ggaaagctct atcgctttga gacgaaagac acggttcaca tcctcaaaaa
     2581 ggacaagcca tgaagggcca catccgcgaa cggtcgcccg gtcattgggc catcgtcatc
     2641 gacgtgcgtg attcccagac cggcaagcgc aagcgccgct ggcactcctt tgccggcacc
     2701 aagcgccagg cccaagtgga agctgcacgt ctgatttccg agctacagaa aggcgcagcg
     2761 gtcgagccga ataggatcac cctcgcccag tttctcgacc aatggctgga atatatcgag
     2821 ccacaggtcg cccctcgaac gcatgagcga tatggcgaga ttgtccgcac ctatctcgcc
     2881 cccgcgctcg gtacgacgtt gttgaccaag atgcaggcaa tgacgatcag cgcggcttat
     2941 gccggaatgc tctctggcgg ccgcaggaag ggcaacggcg ggctttcgcc tcggtcagtg
     3001 catcattgcc atagaatact ctcgcaagcc ctgcagcagg ccgtgcgttg gcgaatgcta
     3061 tctcgcaacc catgcaatga cgttgacccg ccgcgcatcg agcggcgcgc gttgaaagtc
     3121 tgggatgttg ccactatggc gcaggccctg gagtgtgcgc ggtcgcttcg cattttcatg
     3181 ccgatgcttc tcgccgggat gctggggctt cggcggggcg agatcgctgc gttgcgctgg
     3241 cgtcacgttg atctggacag ggcacaggtc gctatagttg agagcttcga gcagacgcgc
     3301 ctcggggtga gggtcaaggc ccccaaatct ggtcgtggcc gcaaagtcgc attgccgact
     3361 agggtcgtgg cggagcttcg cacttggcgc acgaagcagg ctcaggagct gttgaggctc
     3421 ggcatccggc ttaacgatga ctgtttcgtc tgtgctggtg aggatgggca gccgatccag
     3481 ccccaatcgt tgacacacgc atggcatcga tttctggcca ggactaacct tccgcgcctg
     3541 cgatttcatg acttgcggca ttcgcacgca acccacatgt tagcttcggg cgtgcacccg
     3601 aagatcgcct ctgagcgctt gggccatgcg accgttggat tgaccctgga cacctatagc
     3661 catgtgatcc ccgggatgca ggaggatgcc gttgcgcgta ttgacgcggc cttcgccggc
     3721 acggtaggca agcccatcgg gtagcaaaat ggtagcaaaa tagcccccca aacgaaaaaa
     3781 accttttggt cccgtagctc agccggatag agcagcggtt tcctaaaccg aaggtcggag
     3841 gttcgagtcc tctcgggacc gccagaaaat caatggtttg gctgctattc gcgcttgccg
     3901 aaaccggcaa ttgccaccgg agtgccaccc agcgcctccc gcagccacgc atcgacacca
     3961 gcggccgcgt cggcctgcat gttggggatg aacgcggcgt atacggccaa cgttgttgag
     4021 accgaagcgt ggccagcgcg ctcggacacg atcttgatgt gcacgccgtc catgagttga
     4081 tggcttatgt gggtgtgacg aagaccgtga aatgtgattc gtcgcacccc ggtagcccgg
     4141 atcagtttgt cgaacgactt agtgagggtg tcggggtcca aaggcgatcc atcgggatga
     4201 gcaaatacaa gattcaactt taggccgagc cgcagatgct ctgctgcttg ctctgttcgg
     4261 tgacgtcgt
//
LOCUS       DATLJT010001223         1739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1470825,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001223 DATLJT010000000
VERSION     DATLJT010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470825"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VM910_28210"
     CDS             <1..772
                     /locus_tag="VM910_28210"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI66431.1"
                     /translation="RLRSGGRLVYVGAGTSGRLAVQDGAELVPTFSWPQERLLLFIAG
                     GRNALLQSVEGAEDAVDQAARLTQQHDIGSADVVIAVAASGTTPFTLSCLTEAKRRGA
                     LTIGVANNRDTPILNEADHAVMLDTGAEPIAGSTRMKAGTAQRITLNVFSSLVMILLG
                     RVYDGLMVDLQAVSQKLVRRSEGILTRLTGSSGEQAREALHRANGNVKLAVLILHGCD
                     VKQATDVLHRAGGQLRAALADIGKPGPEPADLDDLPTS"
     gene            complement(916..1125)
                     /locus_tag="VM910_28215"
     CDS             complement(916..1125)
                     /locus_tag="VM910_28215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66432.1"
                     /translation="MAQVITRCRLTSHYMFMGMDVDPKEFTRSPGPFARKFCPFCACD
                     HLWHKEDSKFLANEVVLRPHIQQAS"
     gene            complement(1229..>1739)
                     /locus_tag="VM910_28220"
     CDS             complement(1229..>1739)
                     /locus_tag="VM910_28220"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI66433.1"
                     /translation="ARVLQAIRSGPNRAIAVTMMQWTGPMLQVQVVGWTLIGDEESAA
                     AFSHAIERAPRQLFGGGTSISGAIDYGATLFEQNSFRANRRVIDVSGDGSNNRGRPVT
                     LARDEAVAAGIGINGLPILALEPDLDRYYYDSVIGGPGAFVVAARNYETFADAILKKL
                     IIEIAANGR"
BASE COUNT          340 a          519 c          562 g          318 t
ORIGIN      
        1 aagattgcga agcggcggtc ggctggtcta tgtcggcgcc ggaacgtccg gccggctggc
       61 cgtccaggac ggcgccgaac tcgttccgac cttcagctgg ccgcaagagc ggctgttact
      121 ttttatagcc ggcggccgga acgccttgct gcagtcggtg gaaggcgccg aggacgcggt
      181 cgatcaggct gcccggctga cccaacaaca cgatatcggc tctgccgacg tggtcatcgc
      241 agtcgctgcg agcggtacca cgccgttcac cttgtcctgc ctaaccgaag caaagcgacg
      301 gggcgctttg acaattggag tcgcaaacaa tcgcgatacg ccgatcctga acgaggccga
      361 tcatgcagtc atgctggata ccggcgcgga gccgattgcg ggatccacca gaatgaaagc
      421 gggaacggcg cagcgtatca ccctcaacgt cttctcatct ctggtcatga tcttgctggg
      481 gcgggtctac gatggactga tggtggacct ccaggcggtt agtcagaagc tcgtgcgacg
      541 tagcgaaggc attctcacgc ggctcactgg tagcagcggc gagcaggcgc gcgaggcatt
      601 gcatcgtgcc aacggcaacg taaagctcgc cgttctgatt ctgcacgggt gtgacgtgaa
      661 gcaggcaaca gacgtgcttc accgcgccgg tgggcagcta cgggcagcac tcgccgacat
      721 cggcaagccc ggtcccgaac ccgccgatct cgacgatctg ccgacctcgt gatgggaccg
      781 gcgctgggct aaaggactgg tcgacaaata tagcgttgtg acccgagcat ctggcgctgg
      841 tgaagaatta aggtcgcgaa gaaattcctc gcgatggttc gcacttcgtc tctgggaggg
      901 acgacccagg agcgatcagc tcgcttgttg aatatggggc cgcagcacga cctcgttggc
      961 caagaacttc gagtcctcct tgtgccacaa gtgatcgcag gcgcagaacg ggcagaactt
     1021 gcgcgcaaac ggaccgggtg agcgcgtgaa ttccttggga tcaacatcca tgcccatgaa
     1081 catgtaatga ctggtgagcc ggcagcgcgt gatgacctga gccatcgcac tcctccgtcc
     1141 taaataataa aggcgctaag acaacatcgc tggtcgggca aagttccgcg cacgcggagc
     1201 tttgcggctg gggagccgac cttggtggct agcggccgtt cgcggcgatc tcgatgatga
     1261 gcttcttgag gatggcgtcg gcgaacgttt cgtaattccg cgcggcgacc acgaaggcgc
     1321 cggggccgcc gatgacgctg tcataatagt agcgatcgag atcgggctcg agcgcgagaa
     1381 tcggcagacc gttgatccca atgccggctg ccaccgcttc gtcgcgggcg agcgtcaccg
     1441 gccggccccg attgttggag ccgtcgccgg agacgtcgat gacgcgccgg ttggcacgaa
     1501 atgaattttg ctcaaacagc gttgccccgt agtcgatggc gccgctgatg gaagtgccgc
     1561 cgccgaacaa ttggcgcggc gcgcgctcga tcgcatggga aaatgcggcg gcgctttcct
     1621 cgtccccgat cagggtccat cctaccacct gaacctgcag catcggtccc gtccattgca
     1681 tcatcgtcac ggcgattgcg cggttgggtc cggagcggat ggcttgcagc acccgcgca
//
LOCUS       DATLJT010001224         1981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3308898,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001224 DATLJT010000000
VERSION     DATLJT010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3308898"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VM910_28225"
     CDS             <1..438
                     /locus_tag="VM910_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI66434.1"
                     /translation="WLGIFETSKVALIAVGVFFPVYLGVLGAILSVDRKIVEVGRAFR
                     LSGPAMIRRILLPAVLPAYVVALRVGLGLGWMFVVAAELMGASEGLGYLLVDGQQLGK
                     PAQILAAIVVFAILGKVTDWLIEIAAAPLLRWQDAFGRQGGAP"
     gene            440..1225
                     /locus_tag="VM910_28230"
     CDS             440..1225
                     /locus_tag="VM910_28230"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66435.1"
                     /translation="MLALNKLGKTYPNGVHALEQFSAEINLGEIVGIIGGSGCGKSTL
                     LRAVSGLDPATSGTVTLDDAAIRAPHPKIGIIFQEPRLLPWLSVAENIGFGLSDLPTD
                     EKRERIARALVRVGLADKARAWPRELSGGQAQRVAIARALVPQPEVLLLDEPFSALDA
                     FTRRDLQDHLLDLWADTRPTLVLVTHDVEEAVALADRVLVMRPRPGRLFEAININLGR
                     PRDRNAPLFDDFKRHVLTALDRSLDRSVPGRQAESGPGQAMWW"
     gene            1275..1784
                     /locus_tag="VM910_28235"
     CDS             1275..1784
                     /locus_tag="VM910_28235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVI66436.1"
                     /translation="MDAAELRALQAPIKERYKSDPSAAVITLKAKGSIENEGIACKVE
                     TGRALAVAGLHPATGGSGLELCSGDMLLEALVACAGVTLKSVATAIDVPLKVGNVIAE
                     GDLDFRGTLGVDKEAPVGFAEIRLRFEVATDALQDKLDLLLKLTERYCVVYQTIRNGP
                     KVSVSMKRN"
BASE COUNT          304 a          661 c          655 g          361 t
ORIGIN      
        1 tggctcggca tcttcgagac ctcgaaagtc gcgctgatcg cggtcggcgt gttctttccg
       61 gtttatctcg gcgtgctcgg cgcgatcctc tccgtcgatc gcaagatcgt cgaggtcggc
      121 cgcgccttcc gcctgtcggg accggcaatg atccggcgca tcctgctgcc ggcggtgctg
      181 ccggcctatg tcgtcgcgct gcgggttggc cttggccttg gctggatgtt cgtggtcgcg
      241 gccgagctca tgggcgcttc cgaaggactt ggctatcttc tggtcgacgg ccagcagctc
      301 ggcaagcccg cgcagattct cgcggccatc gtcgtctttg cgattctcgg caaggtcacc
      361 gactggctga tcgagatcgc cgccgcgccg ctcctgcgct ggcaggacgc gttcggccgg
      421 caaggcggag ccccttgaga tgctcgcgct caacaagctc ggcaagacct atcccaacgg
      481 cgtgcatgcg ctggagcagt tttccgccga gatcaacctt ggcgagatcg ttggcatcat
      541 cggcggctcc ggttgcggca agtcgacgct tctgcgcgcc gtttcaggcc tcgatcccgc
      601 aacctccggc acggtgacgc tcgacgacgc cgcgatccga gccccgcatc cgaagatcgg
      661 catcatcttc caggagccgc ggctcttgcc gtggctgtcg gtcgccgaga atatcggctt
      721 cggcctctcc gatttgccga cagacgaaaa gcgcgagcgg attgcgcgcg cccttgtccg
      781 cgtcggtctt gccgacaagg ccagggcctg gccgcgcgaa ctgtccggcg ggcaggcgca
      841 acgggtcgcg atcgcgcggg cgctggtgcc gcagccggaa gtgctgctgc tcgacgagcc
      901 gttctcggcg ctcgacgcgt tcacgcggcg cgacctgcag gaccatctgc tcgacctctg
      961 ggccgatacg cgcccgacac tggtcctggt cacgcatgac gtcgaagagg cggtggcgct
     1021 cgccgaccgc gtgctggtga tgcgcccgcg gccggggcgc ctgttcgagg cgatcaacat
     1081 caatctgggg cggccgcgcg accgcaacgc gccgctgttc gacgatttca agcgccacgt
     1141 gctgactgcg ctcgaccgct cgctcgatcg cagcgtgccc ggccggcagg cggaatccgg
     1201 cccggggcag gcgatgtggt ggtgacacgg gcagcgtttt cgattacatc cgcgtcgcaa
     1261 aaaaggagag aacaatggac gccgccgaac tgcgcgcgct gcaggccccg atcaaggagc
     1321 gctacaagtc cgatccgtcg gccgctgtca tcaccctgaa ggccaaaggc tcaattgaga
     1381 acgaaggcat cgcctgcaag gtcgaaaccg gccgtgcgct cgcggttgcg gggctgcatc
     1441 cggcaaccgg cggctccggg ctcgaactct gctccggcga catgttgctg gaggcgctgg
     1501 ttgcctgcgc aggcgtcacg ctgaaatcgg tcgccaccgc gatcgatgtt ccgctgaagg
     1561 tcggcaatgt gatcgcggaa ggcgatctcg attttcgcgg cacgctcggc gtcgacaagg
     1621 aagcccccgt cggctttgcc gagatacgcc tgcgcttcga ggtcgcgacg gacgcgctgc
     1681 aggacaagct cgatctcctg ctcaaactca ccgagcgtta ttgcgtggtc tatcagacca
     1741 tcaggaacgg gccgaaggtg tcggtgagca tgaagcgcaa ctagccaggc gggacccata
     1801 accaccgctg ccacctgccc cggctcgcgt tcgcttggcc ggcacgacat agaatgtccc
     1861 cagagctcac cttcattttg atgctagccc tgcgtatggc gaccgccgca gccttcgtgg
     1921 tgtcggcgtc cttcgtcgcc gagcgcgcgg gtcccgtgat cggcgcgctg attgcgacgc
     1981 t
//
LOCUS       DATLJT010001225         1871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3079941,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001225 DATLJT010000000
VERSION     DATLJT010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079941"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VM910_28240"
     CDS             complement(<1..218)
                     /locus_tag="VM910_28240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66437.1"
                     /translation="MLQIDRRTLSVFGLAVLLPPRFALARDVYPSRPIHIIVGFTPGA
                     AADITARVLGESMGPILGQQIVVENKPGA"
     gene            complement(374..1240)
                     /locus_tag="VM910_28245"
     CDS             complement(374..1240)
                     /locus_tag="VM910_28245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66438.1"
                     /translation="MRLSRKAFLDFVAGASALTILSWSAFIRSALALDYPTRPVHIIV
                     GFPAGTSSDITARLVAQRLSEQLGQQFVVENRTGAGTNVAADSVVHATADGYTLLWVT
                     QTNAINATLYDTLNFNFSHDVQPVAGILRVPAVMMVNPSVPASTVPEFIAYAKANPGK
                     INMSSPGIGSINHVAGELFNMMAGVKLAHIPYRSSQFPDLLSGEVQITFNPLPSSLDF
                     IRAGKLRGLAVTGATRSDALPNLPTLGEFLPGYEATAWFGIGAPKATPSAIVEKLKRS
                     TRGSLMRSSRAA"
     gene            1437..>1871
                     /locus_tag="VM910_28250"
     CDS             1437..>1871
                     /locus_tag="VM910_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HVI66439.1"
                     /translation="MKISILDDYFDTLRTLPSFQKLKGHEVVIWNDHVQDTDALAARL
                     QDTECLVLIRERTKIQADLLERLPRLKLISQRSVYPHIDIAACTSRGVVVSSSQHAGV
                     PCHAAAELAWALILATARQLPQQMAALKAGQWQIGVGTTLRDK"
BASE COUNT          403 a          534 c          542 g          392 t
ORIGIN      
        1 gcgccgggtt tgttctcgac gacgatctgc tggccaagga ttggtcccat gctctcgccg
       61 agcacgcgcg ccgtaatgtc ggcggccgcg cccggtgtaa atccgacgat gatatgaatt
      121 gggcgagacg ggtagacatc gcgcgccagt gcgaaccgcg gtggtaacag taccgcaagg
      181 ccgaatacgg acaaagttcg gcgatcgatc tgcaacattg ttgtccccta tcggttcacg
      241 cctggatatc ttcaaaatca ctccgctttc gcgccggaaa acttgaccac cttggctcat
      301 ttctcggttt cggattcaat gaatttgctg aaatcagcaa tcgacagcgg catcggctca
      361 ccgccaagat cgatcaggcg gcccttgaac tgcgcatcag cgagccccgc gttgatctct
      421 ttagcttctc gacgattgcc gaaggcgtcg ccttgggcgc gccgatccca aaccatgcgg
      481 tggcctcata acccggcaaa aattcgccaa gcgttggaag gttcggcaat gcatccgaac
      541 gcgtcgcgcc agtcacggca aggccgcgca gcttgccggc tctgatgaaa tcaagcgagg
      601 acggcagcgg gttgaaggtg atttgcacct cgccgctcaa cagatcggga aactggctgc
      661 tgcgataggg aatatgcgcg agtttgacgc cggccatcat attgaagagt tcgccggcga
      721 catgattgat gctgccaatc cccggcgacg acatgttgat cttgcccgga ttggccttgg
      781 cataagcaat gaattccgga accgtactcg ccggcaccga cgggttcacc atcatcactg
      841 cgggaacgcg aagaatgccg gcaaccggct ggacgtcgtg gctaaagttg aagttcagcg
      901 tatcgtaaag cgtcgcattg atggcgttag tctgcgtgac ccagagcagc gtgtagccgt
      961 cggcggttgc gtgcacgaca gagtcggcgg ccacattggt gccggcgccg gtgcgatttt
     1021 cgacgacgaa ttgttgcccg agttgttctg acagccgctg cgccacaagc cgcgcggtga
     1081 tgtccgagga ggtcccggct ggaaagccga caatgatatg aaccggacgg gtgggataat
     1141 cgagtgcaag cgcactgcga atgaatgcgc tccaactgag gatcgtcagc gcgctcgcgc
     1201 cggcaacaaa atccaaaaac gctttgcggc tgagcctcat gatgaagcaa gctacactaa
     1261 tcctccgatg gcgtgtagag atgcggctaa accgaaatga ctttaagttg atcaccattt
     1321 cgctttagct tcttgtttga gcatgatctt ttcggaaaaa ccggtatcca cttttccgga
     1381 tcatgctatt gaacgccggg ccctattttg atccttaccg gcgcaaaggt cggctcatga
     1441 agatctcgat cctagatgac tattttgaca cgctgcggac tctgccttcc ttccagaagc
     1501 taaaagggca cgaggttgtc atctggaacg atcatgtgca ggacactgat gcgctggcgg
     1561 cgcgtttaca ggacaccgaa tgcctggtgc tcattcgcga gcgcaccaaa atccaggcgg
     1621 atctgcttga acggctgccg cgccttaaac tcatcagcca acgcagtgtc tatccgcata
     1681 tcgatattgc cgcctgcacg agccgcggcg ttgtcgtatc gtcgagccag cacgctggcg
     1741 tcccttgtca tgccgcagcc gaattggcct gggcgctcat tctggcgacg gcgcgtcaat
     1801 tgccgcaaca gatggcggcg ctcaaggccg gccaatggca gatcggcgtc ggcactacct
     1861 tgcgcgacaa g
//
LOCUS       DATLJT010001226         3491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2390013,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001226 DATLJT010000000
VERSION     DATLJT010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390013"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1204
                     /locus_tag="VM910_28255"
     CDS             116..1204
                     /locus_tag="VM910_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66440.1"
                     /translation="MTTRRDFIKAAGASGIVFCGCAMRDVARSQGSDGQRLPVTVNGK
                     RIKTIDVHAHCHFHEALALMGDEAGRVMPRVKGAMEHFIIIEERLKGMDAQAIDMEVL
                     SINPFWYRKDRDTAAKIVELQNEKLAALCASRPDRFAAFASLALQYPDLAVRQLETAV
                     KKQGLRGAAIGGSVLGEEFSDPKFHPVWAKAEELGAVLFIHPQSTPELARRFKGNGWL
                     SNTIGNPLDTTIALQHLIFEGTLDRFPGLKIIAAHGGGYLGSYAARGDHACFVAPQNC
                     NPHMKLKKPPSDYLKQLYFDALVFTPEALRHLVAQVGASQVVIGSDHPIPWEQHPVDH
                     VFATTTLSDEDKIAILGGNAARLFGMQG"
     gene            1215..2108
                     /locus_tag="VM910_28260"
     CDS             1215..2108
                     /locus_tag="VM910_28260"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI66441.1"
                     /translation="MDLDSATPEFKRIVGANPPLDQIAQGTLFGEGPVWDRRRKRFFW
                     VDIIGDTIYQWVPGTGKSVLVHPSRHANGMTFDLEGRLTIAGWSQRTIWRIEHDGSFT
                     TLASRWQGKKFNSPNDIVVRSDGSIYWTDSAGGLVIPGMVGEDMQRQLDIQAVFRITP
                     QGKVELAIEDTVYPNGLCFSPDERILYVNDTRMGHIRAFDVAHDGSVSNGRLFHQLTG
                     SEPGVADGMKCDCEGNVYCTGPGGVHVIDPRGKLLGRLFIPGGHTTNIAWGDDDWRSL
                     YMVTYHSVFRTRLNIPGVPVW"
     gene            2118..3020
                     /locus_tag="VM910_28265"
     CDS             2118..3020
                     /locus_tag="VM910_28265"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI66442.1"
                     /translation="MAWEFERVAGPFEGSAGGVIWDSTRDQVLFSTVDEGRLLWLDHA
                     SKAVSEFRRYANRVNGLARGPNGEIYGAQEGGRRLVEFTPDGRTVAVDALLDGKYHNQ
                     PSDLVVDRQHRIYFTDPRHAVIPFGPSIFPFLDHCSVLRLERNDRRAWTASRLTYDTV
                     SPRAVLLSHDQNTLYVADGQPRPEQRRELRAYPIRADGTLEHPIVLHVFGEDHRGQHR
                     GIEGMCLDCDGNIVAVGGWQRSGPGPLVYVFAPTGAVIETHPIPADLPNKCCFGGPGL
                     DTLYVTTGGGHLYRAAAGGRRGSL"
     gene            3049..>3491
                     /locus_tag="VM910_28270"
     CDS             3049..>3491
                     /locus_tag="VM910_28270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66443.1"
                     /translation="MMGRLLAALLSFLVLGGGAHAQDYPHRVIRIVVGPGPDIVARLF
                     STKISDTLGQSVIVEQRPGAGGVIAAQTVAAAPPDGYTLLQATASYTINTALQSSPLD
                     IGRDFGPVALVSTIPFVLVLHPSVPAKTISDLITYAKANPGKLNYA"
BASE COUNT          633 a         1120 c         1100 g          638 t
ORIGIN      
        1 ggtatcgaaa agctgaaggg cgaagctgcg cggcagtgaa gactgctgtc acggcgcgca
       61 tccgccgcag atgtgaaggc gcatgaaaca cgaatatcga gggaggggga ccgccatgac
      121 tacgcggcgc gactttatca aggccgcggg cgcatcgggc attgtgttct gcggctgcgc
      181 gatgcgcgat gtggcgcgga gccaggggtc cgatggccaa cgtctgcccg tgactgtcaa
      241 cggcaagcgg atcaagacca tcgatgtgca cgcgcactgc cacttccacg aggcgctcgc
      301 tctgatgggg gacgaggcag gccgcgtgat gccgcgcgtg aaaggtgcca tggagcattt
      361 catcatcatc gaggagcgcc tcaaggggat ggacgctcag gcgatcgaca tggaagtgct
      421 ctccatcaac ccgttttggt accgcaagga ccgcgacacg gccgccaaga tcgtcgagct
      481 gcagaacgaa aaactggctg cattgtgcgc ctcgcgcccg gatcgctttg cggcatttgc
      541 ctcgctcgcg ctgcaatatc cggacctcgc cgtgcggcag ctcgagacgg cggtgaagaa
      601 acagggcctg cgcggagccg ccatcggcgg cagcgtgctt ggggaagagt tttccgatcc
      661 gaagtttcat ccggtgtggg cgaaggcgga agagcttggg gcggtgctgt ttatccatcc
      721 gcaaagcacg ccggagctcg cccggcgctt caagggcaac ggctggctgt cgaacactat
      781 cggcaatccg ctcgatacga cgattgcgct gcagcatctg atcttcgaag gaacgctcga
      841 tcgattcccg ggcctgaaga tcatcgcggc gcacggcggc ggctatctcg gctcctacgc
      901 ggcgcgcggc gaccatgctt gcttcgtcgc gccacagaat tgcaacccgc acatgaagct
      961 gaagaagccg ccgagcgact atctcaaaca gctctatttc gatgcactcg tattcacgcc
     1021 ggaagcgctc cgccacctgg tggcgcaggt cggtgccagc caggtcgtca tcggcagcga
     1081 ccatccgatt ccgtgggagc agcatccggt cgatcatgta ttcgcgacta cgacgctgag
     1141 cgacgaagac aagatcgcca ttctgggcgg caatgcggca cgcctgttcg gcatgcaagg
     1201 ctaggagcgc gccggtggat ctcgactcgg ccacgccgga attcaaacgc atcgtcggcg
     1261 ccaatcctcc actcgaccaa atcgctcagg gcactctttt cggcgaaggt ccggtctggg
     1321 accgccgccg gaagcgcttc ttctgggtcg atatcatcgg cgacacgatc taccagtggg
     1381 tgcccggtac ggggaaatcg gtgttggtgc acccctcgcg tcacgccaat ggcatgacct
     1441 tcgatctcga aggtcggctc accatcgccg gctggtcgca gcgcaccatc tggcgcatcg
     1501 agcatgacgg ttcgttcacg acccttgcgt cgcgctggca gggcaagaaa ttcaatagcc
     1561 caaacgacat cgtcgtgcgc tctgacggct cgatctattg gaccgattcc gccggcggcc
     1621 tcgtcattcc cggcatggtc ggcgaggaca tgcaacgcca acttgacatc caggcggtct
     1681 tccgcatcac gccgcagggc aaggtcgaac tcgccatcga agacaccgtc tatcccaacg
     1741 gactgtgctt cagcccggac gagagaatcc tctacgtcaa tgatacgcgc atggggcata
     1801 tccgcgcctt cgatgtcgcg cacgacggca gcgtcagcaa cggccgccta ttccaccagc
     1861 tcaccggcag cgagccgggc gtcgccgacg gcatgaagtg cgactgcgaa ggcaacgtct
     1921 actgcaccgg gcccggcggg gtgcatgtca tcgacccgcg tggcaagctg ctcggccgcc
     1981 tgttcattcc cggcgggcac accaccaata tcgcttgggg cgatgacgat tggcgctcgc
     2041 tctacatggt gacttatcac tcggtgtttc gcacgcgtct caacatcccc ggcgtgccgg
     2101 tctggtagga gcgcagcatg gcatgggaat tcgaacgcgt tgccgggccg ttcgaggggt
     2161 ccgccggcgg tgtcatctgg gatagcaccc gcgaccaagt gctgttcagc accgtggatg
     2221 aggggcggct gctctggttg gaccacgcga gcaaggcagt gagcgaattc cggcgctacg
     2281 ccaaccgcgt caacggcctc gcgcgcggac cgaacggtga gatatacggc gcgcaagagg
     2341 gcgggcgccg gcttgtcgag ttcacccccg atgggcgcac agtcgcggtc gatgcgctcc
     2401 ttgacggcaa gtaccataat cagccgagcg accttgttgt cgatcggcag caccgcatct
     2461 atttcaccga tccccgccat gccgtcattc cgtttgggcc atcgatcttt ccattccttg
     2521 accactgctc ggtgttgcgc cttgaacgca acgaccgccg cgcctggacg gcgtcgcggc
     2581 tcacctacga caccgtcagc ccccgcgcgg tgctgctctc gcacgaccag aacacgcttt
     2641 acgtcgccga cggccagccg cgtccggagc agcggcgcga gctgcgtgcc tacccgatcc
     2701 gcgccgacgg cacgctcgag catcccatcg ttctgcacgt tttcggcgaa gatcatcgcg
     2761 gccagcatcg cggaatcgag ggcatgtgcc ttgattgcga cggcaacatc gtcgcggtcg
     2821 gcggctggca acgcagcggg cctggtccgc tggtgtacgt ttttgcgccg accggcgcgg
     2881 tgatcgaaac tcatccgatc ccggcggacc tgccgaacaa gtgctgtttc ggcggtcctg
     2941 gccttgatac gctttacgtc acgaccggcg gcggccatct ctatcgtgcg gcggccgggg
     3001 gtcggcgcgg gtcgctctga agtttccgcg ggccaagaga aacaaggcat gatgggtcga
     3061 ctgctcgctg ccttgctgtc gttcctagtg ctgggcggcg gggcgcatgc gcaggattat
     3121 cctcatcgcg tgatccgcat cgtggtcggt cccggccctg atatcgtcgc gcgcttgttc
     3181 agcacgaaga tcagcgacac gctcggccag tcggtcatcg ttgaacagcg tcccggtgcc
     3241 ggcggcgtga tcgcggcgca gaccgttgct gcagctccgc cggacggtta cacgctgctg
     3301 caagcgaccg cttcctacac aatcaatacg gcgctgcaat cctcgccact cgatatcggc
     3361 agggatttcg gtccggtggc gctagtcagt accatcccat tcgtgctcgt gctgcatccg
     3421 tccgtgccgg cgaagacgat ttcggacctc atcacctatg ccaaggcgaa tcccggcaag
     3481 ctgaactacg c
//
LOCUS       DATLJT010001227         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3309597,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001227 DATLJT010000000
VERSION     DATLJT010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3309597"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VM910_28275"
     CDS             <1..717
                     /locus_tag="VM910_28275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66444.1"
                     /translation="HACENRDETVASHLKYGMGDIARLEKLGLLGPNLLLLHMGWVDA
                     KELHMLQKRDVKISLAPSASMHQAMGNISHGKAPEMLELGLSLSLGSDSAMSSNYLDA
                     IRQAFLLVGGLHEARLDPKILRPETAVEMLTINGARCMQWDQDLGSLEVGKKADVTLL
                     DIMRPEWQPVHNPIANLVYCAHGGCADTVIVDGKILMRGSKVLTLNEHDLYEEAQDRA
                     TSLIRRTGLERVVASTWPMH"
     gene            complement(804..1001)
                     /locus_tag="VM910_28280"
     CDS             complement(804..1001)
                     /locus_tag="VM910_28280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66445.1"
                     /translation="MAVVISRSSNQSLQLSSPLLQRSMLDEFAHPSTGLRELHGFLEL
                     DQARLTAGKQLLNVVDRELGY"
     gene            1000..2172
                     /locus_tag="VM910_28285"
     CDS             1000..2172
                     /locus_tag="VM910_28285"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI66446.1"
                     /translation="MSMSSLKISGGEVFLVALPIRRPHHWVGHTARLGEGYAVLRLEL
                     ESGIVGWGEAQLIGTWGGDHGARYGETPKMTATAIKEILIPAIAGASVTEIEQVHARM
                     NRALRGYPYAKAAIDVALHDAIGKIYGVPVYQLLGGKVHDGTALAHSIGIMDADAAAR
                     EAAEVIAEGITTIKVKIGVEAERDIKVVAAVRQAIGNAGKIRVDANQGYRTWREAVRV
                     IAAMEKYDLVYVEQPVEGLRAMAEVSARINVPVMADESVWTAEDVLEIVRLNAAQMLS
                     VYYTKPGGLAHAKRLLAVAGAASLPCDINGSAEMGIGVAADLHLAVSSPEIVLPGTIP
                     VTSTAEMVVTKVAGHKYLDDLIKTPFRFEKGFLYVPDGPGLGIEVDEAKIAKHRIG"
     gene            2169..>2396
                     /locus_tag="VM910_28290"
     CDS             2169..>2396
                     /locus_tag="VM910_28290"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVI66447.1"
                     /translation="MIDVPPIKSIAIIGAGDGGCAAAAQLAQRGFDIRLYGRSRATTE
                     PLSATGGIEYEGALGEGFAPLSLITNDAGTAI"
BASE COUNT          503 a          722 c          746 g          425 t
ORIGIN      
        1 cacgcctgtg agaatcgcga cgaaaccgtc gcctcgcatt tgaagtacgg catgggcgac
       61 atcgctcggc tcgaaaaatt ggggctgctt gggccaaact tgctgctgct ccatatgggc
      121 tgggttgatg caaaggaact acacatgctg cagaagcggg acgtgaaaat ttccctcgcg
      181 cccagcgcgt cgatgcatca ggcaatgggc aacatcagcc atggcaaggc gcccgaaatg
      241 ctggaactcg gtctttccct gtcgctgggg tcggattcgg ccatgtcgag caactatctg
      301 gacgctatcc ggcaagcgtt tctcctcgtg ggcggcttgc atgaagctcg tctcgatccc
      361 aagatacttc gccccgagac ggcggtcgag atgctgacga tcaacggcgc ccggtgcatg
      421 cagtgggacc aggatcttgg ctcactcgaa gtcgggaaga aagccgacgt cacgttactc
      481 gacatcatgc ggcctgagtg gcaacccgtt cacaatccaa ttgcaaacct cgtctattgc
      541 gcacacggcg gatgcgccga caccgttatt gtcgatggca agatcctcat gcgcggcagc
      601 aaggtcctca cactcaacga acacgatctg tacgaggaag cacaggatag ggcgacctca
      661 ctgatccggc gcaccggtct cgaacgagtc gtggcctcaa catggccgat gcattagcgc
      721 ttcagatgac acatgtgagg cgctcaataa gatacgcacg ccagcccgaa ccaggtgcag
      781 aatgaaatgg cgaaggagtt gcatcaataa ccgagttcgc gatcgacgac gttcagtaac
      841 tgctttccgg ccgtcagccg cgcttgatcg agctccagga aaccgtgcag ttcgcgcagc
      901 cccgtgctcg ggtgcgcaaa ctcatcgagc attgatcgct gaagtagcgg tgatgagagc
      961 tgcaaacttt ggttagaact ccgcgagatg accactgcca tgagcatgtc cagcctgaaa
     1021 atcagtggcg gcgaggtctt tctcgtcgcc ttgcccatcc gccggccgca tcactgggtc
     1081 ggccatacgg cgcgcctcgg cgaaggctat gcagttcttc ggctggagct tgaaagtggc
     1141 atcgtgggtt ggggcgaagc gcagttgatc ggcacatggg gcggcgacca tggcgcgcgc
     1201 tatggagaga cgccgaagat gactgcgact gccatcaagg agatcctgat tcccgccatc
     1261 gccggtgcga gcgttaccga gatcgagcaa gtgcacgccc gcatgaatcg ggcgctacgc
     1321 ggctatccct acgcgaaggc cgcgatcgat gtcgccctcc acgatgcgat cggcaagatc
     1381 tacggtgtcc cggtctatca actcctgggc ggcaaggttc acgacggcac ggcgctggca
     1441 cacagcatcg ggatcatgga cgccgacgct gccgcgcgcg aggcggccga ggtgatcgcg
     1501 gagggcatca ccacgatcaa ggtcaagatc ggcgtcgaag ccgagcgcga catcaaggta
     1561 gtggccgcgg tgcgccaagc gatcggaaac gccgggaaga tccgggtcga tgccaaccaa
     1621 ggctaccgca cctggcgcga agccgtgcga gtgattgctg ccatggagaa atacgatctt
     1681 gtctatgtcg agcaaccagt cgaagggctg cgggccatgg cggaagtaag cgcgcgcatc
     1741 aacgtcccgg tgatggccga tgagagtgtc tggaccgccg aggacgtgct tgagattgtc
     1801 cggctcaacg ccgcgcagat gctttcggtc tactatacca agccaggcgg gctcgcgcac
     1861 gccaagcgac tcctggcagt tgccggcgcg gcatcgctgc catgcgacat caacggctcg
     1921 gcggaaatgg ggatcggcgt cgctgctgat ctgcatctcg ccgtctcgtc gcccgagatc
     1981 gtgctccccg gcacaatccc ggtcacctcg accgccgaaa tggtcgtgac caaagtggcg
     2041 gggcacaaat atctcgacga tctgatcaaa acgccgttcc gtttcgaaaa ggggttcctc
     2101 tacgtgccgg atggaccggg cctcggcatt gaggtggacg aggcgaaaat cgcgaaacat
     2161 cgcatcggat gatcgacgtg ccgccgatca agagcattgc gatcatcggc gcaggcgacg
     2221 gcggctgtgc cgcagccgcg cagctggcgc agcgcggctt cgacatacgt ctctacggcc
     2281 gcagccgcgc cactaccgag cctttatccg ccaccggcgg catcgaatac gaaggcgcgc
     2341 ttggtgaggg cttcgcgccg ctgtcgctca tcaccaacga tgccggcacc gcgatc
//
LOCUS       DATLJT010001228         2548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2850856,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001228 DATLJT010000000
VERSION     DATLJT010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850856"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VM910_28295"
     CDS             <1..568
                     /locus_tag="VM910_28295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66448.1"
                     /translation="NINDLPEFLDVWNSHFGEHPCALTVVATKGLALLESILEINIFG
                     VREGAKTTKQIVDHKPSQHMRLGPAAVCAGDLLCLSGLYAADSDGAIPQARSTAGLKY
                     FGSPTHHEMQAILTAADDICRTGGTSLSNLLRVNHFLSDLNVVPCALRSWQEKLAGAP
                     IPFGAVRTPAPMPVPGCNIVADMWVYHP"
     gene            855..1823
                     /locus_tag="VM910_28300"
     CDS             855..1823
                     /locus_tag="VM910_28300"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66449.1"
                     /translation="MPMRIALPSMMLALATVTMVSAASGADTVKVAIGQIDAWANQAP
                     TLGMKAGIFRKHGIVLETFGTQGAGETLQAVISGSADIGIGIGTAGAMRAFARGAPVR
                     AIAAAYVGVGDQYWYVPANSPLQRLTDATEKHTMAYSTNGATSHVITLGFVKQLGVKA
                     KPTATGGPPATYTMTMTGQIDIGWAVVPFGLAELQAGKIRVIARGPEVPTLRNQTIRV
                     QVANANALKERKDAMVRFMRAYRESLDWMYANPLAVKYYAETIKQPEELVVMQRDQYN
                     PKDALSPDRLSDLDQVMADAVEMKFLDAPLTKEQLAEFFQIPPPGT"
     gene            1965..>2548
                     /locus_tag="VM910_28305"
     CDS             1965..>2548
                     /locus_tag="VM910_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66450.1"
                     /translation="MLAGKSSAASATRRLPNREAALEQLYSDISAKNMFPFWATSTDV
                     AHDEIKQLMGTQKAVPHLWSYKSDIEPILFRAAELVTMDDSERRSLILVNPGLSPRRA
                     TVSTMYTAYRLNDANEVMPPHRHSPNAIRFGLTGKGNFTGVGGEDITFGPGDMVLTPV
                     DTWHNHGNVGNEPAVNLSVLDLPLVETLNAVYFE"
BASE COUNT          498 a          786 c          781 g          483 t
ORIGIN      
        1 caacatcaac gatctgcccg aatttttgga tgtttggaac agccatttcg gcgagcatcc
       61 gtgtgcgctc accgtcgttg cgaccaaggg ccttgctctc ctcgagtcga tcttggagat
      121 caacatcttc ggtgttcgcg aaggcgccaa aaccacaaag cagatcgtcg atcacaagcc
      181 gtcccaacac atgcggctcg gtccggcagc ggtgtgcgcg ggcgacctgc tgtgcttgtc
      241 ggggctctat gccgccgatt cggatggagc aatcccgcaa gcgcgcagca cggccggctt
      301 gaaatatttt ggctcgccca cccatcacga gatgcaggca atattgacgg ccgctgacga
      361 catctgtcgc acgggaggta cctcgctctc gaatttgctt cgggttaatc atttcctcag
      421 cgatctcaat gtcgttcctt gcgcgttgcg cagctggcag gaaaagctcg cgggtgcgcc
      481 gatcccgttc ggcgcggtgc gtacgccggc tccgatgcct gtgcccggct gcaatattgt
      541 ggcggacatg tgggtctatc atccgtaatg cgagggcaat ctcgaaggga cgcttttcac
      601 ggaacgtccc cgtcgtgcgg agagctgcat actgctgaga acgaaaatca tatttggtcg
      661 agggcggcgt tctaccttgg gtttcgcgca agcagaaatt cgttccgctc atcctcggca
      721 gtccgtggtc cgatagcttc cgttgcggct tacaagcaat ccggcaattg ctattgtcgc
      781 acgccggtcc ggttgaaccg gctttgcgcg tttcgtcgtg atgcaccgac cgtcacggct
      841 gtttggaggt gacgatgccg atgcggattg cgctaccttc gatgatgctg gcgttggcga
      901 ctgtgacgat ggtgtcggcg gcgagcggcg ccgataccgt gaaggtcgcg atcggccaga
      961 tcgatgcctg ggcgaaccag gcgcccacgc tgggcatgaa ggccggcatc ttccggaagc
     1021 acggcatcgt gttggaaacc ttcggcaccc agggtgccgg cgagacattg caggcggtga
     1081 tctccggttc ggccgacatt gggatcggca ttgggaccgc aggggccatg cgagccttcg
     1141 cgcgcggcgc gccggtgcgc gcgatcgcgg ccgcctatgt cggcgtcggc gatcaatatt
     1201 ggtacgtacc cgccaattcg ccgctccagc gtctgacaga cgcaaccgaa aagcacacga
     1261 tggcctactc gaccaatggt gccacctcgc acgtgatcac gctcggcttc gtcaagcagc
     1321 tcggcgtcaa ggcgaagccg accgcgaccg gcggcccacc cgccacttat acgatgacga
     1381 tgaccggcca aatcgacatc ggctgggcgg tggtcccgtt cggcttggcc gaattgcagg
     1441 cgggcaagat ccgggtgatc gcccgcggcc ccgaggttcc gaccctgcgg aaccagacca
     1501 ttcgggtgca agtcgccaac gccaacgcac tcaaggaacg caaggacgcg atggtccggt
     1561 tcatgcgcgc ctaccgcgag tcgctcgact ggatgtatgc gaacccgctc gccgtcaagt
     1621 actacgccga gacgatcaag cagccggagg agctggtcgt gatgcagcgc gaccagtaca
     1681 acccgaagga cgcattgtcg ccagaccggc tctcggacct cgatcaggtg atggcggacg
     1741 ctgtggagat gaagttcctc gatgcaccgt tgaccaagga gcaactcgcg gagtttttcc
     1801 aaattccgcc accgggaacg tgacggcatc gtcccctcgg ccaggcggcg cgttcgcgca
     1861 tgcgcggctc aacgcgagcg gatggcgcgt gcgattgacc aggcaaccgc ctgtgttacc
     1921 ttgcatcgaa tggctcactc cagccgggtg aaggagcgac cgacatgttg gcaggtaaga
     1981 gcagcgcagc gagcgcgaca cgccgtcttc cgaatcggga agcggctctc gagcagcttt
     2041 acagcgatat ttccgcaaaa aacatgttcc cgttctgggc gacgtcaacc gacgtcgcgc
     2101 atgacgaaat caagcaattg atgggcacgc aaaaggccgt tccgcatctg tggagctaca
     2161 aaagcgatat cgaaccaatt ctatttcgag cggcggaact ggtaacgatg gatgattccg
     2221 aacgccgctc gctcatcctg gtcaatcccg ggctctctcc gcggcgcgcg accgtgagca
     2281 cgatgtacac cgcctatcga ctcaatgatg ccaacgaggt gatgccgccg caccggcact
     2341 cccccaacgc catccggttc ggcttgaccg gaaaaggcaa tttcaccggc gtcgggggcg
     2401 aggatatcac cttcgggccc ggcgacatgg tgctcacccc ggtcgacacc tggcacaacc
     2461 acggcaacgt gggaaacgag ccggcggtca atctttccgt gctcgatctg ccgctcgtcg
     2521 agacgctcaa tgcggtctac ttcgaaca
//
LOCUS       DATLJT010001229         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1011183,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001229 DATLJT010000000
VERSION     DATLJT010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011183"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VM910_28310"
     CDS             <1..1194
                     /locus_tag="VM910_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HVI66451.1"
                     /translation="IGAAIGAVIARRVPMTSMPELVAAFHSLVGMAAVLVAAGAFYAP
                     EAFDIGTPGNIHPQSLVEMSLGVAIGALTFTGSVIAFLKLSARMSGAPISLPFRHIIN
                     IALAVALAFFIVGLVVSGSAFDFWMIVILALALGVLMIIPIGGADMPVVISMLNSYSG
                     WAAAGIGFTLGNSALIITGALVGSSGAILSYIMCHAMNRSFISVILGGFGGETAAVAG
                     PGGEQKPAKLGSADDAAFIMKNASKVIIVPGYGMAVAQAQHALREMADTLKKEGVEVK
                     YAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQTDVAFVIGANDVTNPAAE
                     EDPKSPIYGMPVLQVWKAGTVMFIKRSLASGYAGIDNPLFYRDNTMMLLGDAKKMTEN
                     IVKAM"
     gene            1307..2113
                     /locus_tag="VM910_28315"
     CDS             1307..2113
                     /locus_tag="VM910_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66452.1"
                     /translation="MTVLKWIVSVAALGYLGAIALLFVGQRALVFPVPTTLRTTPRAA
                     GFPHAEEQVLTTSDGERVIVWHVAAAPGRHVVIYFPGNGDTLAGSAGRFADITSDGTG
                     LVALSYRGYAGSSGRPSEKGLLQDAAAAYAFASALYPASKLVLWGFSLGSGVAVALAA
                     DRQAGGVILEAPYISIADVAAAAFPFLPVRYFVKDPFHSDRRIAKVTVPLLIMHGARD
                     ATIPIALGERLFALAHEPKQFVRFDQGGHDDLGNFGAIETARQFIGALKG"
     gene            2142..3239
                     /locus_tag="VM910_28320"
     CDS             2142..3239
                     /locus_tag="VM910_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HVI66453.1"
                     /translation="MSAHGPMPKSAWIFPALSVVLFAVATAAHYNFTPSPFGLVFAAV
                     LLVILFGTVFAAVHHAEMIAHRIGEPFGTLLLTLSVTIIEVALITTIMLGEKEVPTLA
                     RDTVFAVVMIVCNGLVGICVFIGGVQYREQGYQVSGANLYLSVLFTMATITEVMPDYT
                     LTTPGPIYSAAQLAFVSVVTIFLYGVFLYTQTIRHRDYFVTEDAGFTEDAEPISMARL
                     GLDFALLLISLLGVILLAKKFSLVIDAATAAIGAPPAFAGVVVALLILLPESVAAVAA
                     ARKNELQKSINLALGSSVATIGLTIPAVAVAAYLLDKQLVLGLNQQNIVLLLLTFVLS
                     MLTFGTGRTNMLFGLVHLVVFAVFVFMVFVP"
     gene            3260..3544
                     /locus_tag="VM910_28325"
     CDS             3260..3544
                     /locus_tag="VM910_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66454.1"
                     /translation="MLGAKSQVQIDTPEVRVTEWRLAPGSATGHHIHEMDYVIVPVTS
                     GEMTIVAPNGDRSKAQLGAGKSYFRKAGVEHDVLNETSSEIVFLEVELKP"
     gene            3637..>4064
                     /locus_tag="VM910_28330"
     CDS             3637..>4064
                     /locus_tag="VM910_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66455.1"
                     /translation="MLNYLKKFALDILPSVAATIIGAYIVNHYIATKPEKPAAAVSAA
                     ASKADPRKAVAKTELKSEAKADPAKSAQVPAETASIPEPGIKAKGISERVMIENRAAE
                     GPAEVKPVEKPADVKAVETKPEAKPEAKPAETASAPVETRR"
BASE COUNT          693 a         1315 c         1250 g          806 t
ORIGIN      
        1 atcggtgccg cgatcggcgc ggtgatcgcg cggcgcgtgc cgatgacctc gatgccggaa
       61 ctggtcgccg ccttccattc gctggtcggc atggccgcgg tgctggtcgc tgccggtgcg
      121 ttctatgcgc ccgaggcgtt cgacatcggc acgcccggca acatccatcc gcagagcctg
      181 gtcgagatgt cgctcggcgt cgccatcggc gccttgacct tcaccggctc ggtgatcgcg
      241 ttcctgaagc tgtctgcgcg gatgagcggc gcgccgatca gcctgccgtt ccgccacatc
      301 atcaacatcg cgcttgcggt cgcgctggcg tttttcatcg tcggcctcgt cgtgtccggc
      361 agcgcgttcg acttctggat gatcgtcatc ctggcgctgg cgctcggcgt gctcatgatc
      421 atcccgatcg gcggcgccga catgccggtc gtgatctcga tgctgaactc ctattccggc
      481 tgggccgcgg ccggcatcgg tttcacgctt ggcaactccg cgctgatcat caccggcgcg
      541 ctggtgggtt cctccggcgc gatcctgtcc tacatcatgt gccatgcgat gaaccgctcc
      601 ttcatctcgg tcatcctcgg cggttttggc ggcgagaccg cagccgttgc cggccccggc
      661 ggcgagcaga agccggccaa gctcggctcg gccgacgacg ctgccttcat catgaaaaac
      721 gcctccaagg tcatcatcgt gcccggttac ggcatggcgg tggcgcaggc gcagcacgca
      781 ctgcgcgaaa tggccgacac cctgaagaag gaaggcgtcg aggtgaagta cgccatccat
      841 cccgtggccg gccgcatgcc cggccacatg aatgtgcttt tggcagaggc caacgtgccc
      901 tatgacgagg tgttcgagct ggaggacatc aactcagaat tcgcccagac cgacgttgcc
      961 ttcgtgatcg gcgccaacga cgtcaccaac ccggccgcgg aagaagatcc aaagtcgccg
     1021 atctacggca tgcccgtgct gcaggtctgg aaggcaggca cggtcatgtt catcaagcgt
     1081 tcgctggctt ctggctatgc cggcatcgac aatcctttgt tctatcgcga caacacgatg
     1141 atgctgcttg gcgacgccaa gaagatgacc gagaacatcg tcaaggcgat gtgagccgca
     1201 agcttgaccc tgcagtggac gcagcggcag tggatcagat gacgcgccgc ggcacgcgac
     1261 ggcgtcgctg catgccatca agcacggacg ctccggtcag cgatcgatga cagtactgaa
     1321 atggatcgtc agtgtcgccg cgctcggcta tctcggcgcc atcgcgctgc tgttcgtggg
     1381 gcagcgcgca ttggtgtttc cggttccgac cactttgcgc accacgccgc gggcagccgg
     1441 ttttccccac gccgaagagc aggttctgac gacatccgac ggcgaacgcg tgatcgtctg
     1501 gcatgtcgcg gcggcgccgg gacgccatgt cgtgatttac ttcccgggca atggagatac
     1561 tttggcaggt agcgccggcc gctttgcgga catcacctcc gacgggaccg gtctcgtggc
     1621 gctgtcctac cgtggttacg cgggttcgag cgggcggccg agcgagaaag gcctcctgca
     1681 ggatgcggcc gccgcctatg ccttcgcgtc tgcgctctat ccggcaagca agctcgtgct
     1741 ctggggcttt tcgctcggct cgggggtcgc ggtggcgctc gcggccgatc ggcaagcagg
     1801 cggcgtgatc ctggaagcgc cctacatttc gatcgccgac gttgcggcgg ccgcctttcc
     1861 cttcctgccg gtacgttatt tcgtcaagga tccctttcat tccgatcggc gcatcgcgaa
     1921 ggtcacggtg ccgctcctga tcatgcatgg cgcgcgtgac gcgacgatcc cgatcgcgct
     1981 cggcgagcgg ctgtttgcat tggcccatga gccgaagcaa ttcgtgcggt tcgaccaggg
     2041 cggacacgac gatctcggca actttggtgc catcgaaacg gctcgacaat tcattggcgc
     2101 tttgaaaggg tgatggccgc aaattttgag gttggtcccg catgagcgcg cacggcccga
     2161 tgccgaaatc ggcgtggatt tttcccgctt tgtccgtcgt cctgttcgcg gtcgcgaccg
     2221 cggcacacta caacttcaca ccgtcgccct tcggcctcgt cttcgcagcc gtgctgctcg
     2281 tcatcctgtt cggcactgtg ttcgctgccg ttcaccatgc cgagatgatc gcgcaccgga
     2341 tcggcgaacc cttcggtacg ctgctgctca cactgtcggt caccatcatc gaggtcgccc
     2401 tcatcacgac catcatgctc ggcgaaaagg aggtgccgac gcttgcccgc gacacggtgt
     2461 tcgccgtggt gatgatcgtc tgcaatggtc tcgttggaat ctgcgtcttc atcggcggcg
     2521 tgcaatatcg cgagcagggc taccaggtct cgggcgccaa tctctatctc agcgtgctgt
     2581 tcacgatggc gaccatcacg gaggtcatgc ccgactacac gctgaccacg ccgggaccga
     2641 tttattccgc ggctcaactt gcctttgtca gtgtcgtgac catcttcctg tatggcgtgt
     2701 ttctctacac ccaaaccatc cggcatcgcg actatttcgt caccgaggac gcgggtttca
     2761 ccgaggacgc ggagccgatc tcgatggcga ggctggggct cgacttcgcg ctgctcctga
     2821 tctcgctgct gggcgtgatc ctgctggcga agaaattttc tctcgtcatc gatgcggcaa
     2881 ctgccgccat cggcgcgccg ccggcttttg ccggcgtggt cgtggcgctc ttgatcctgt
     2941 tgccggagag cgtcgcggcg gtcgccgctg cccgcaaaaa cgaactgcag aagagcatca
     3001 acctcgcgct cggctcctcg gttgcgacca tcgggctcac tattccggcg gtcgcggtcg
     3061 cggcctacct cctggacaag caactggtgc tgggcctgaa ccaacagaat atcgtgctgt
     3121 tgttgctgac cttcgtcctg agcatgctga ctttcggcac aggaaggacc aacatgctgt
     3181 tcgggctcgt ccatttggtg gtgtttgcgg tcttcgtgtt catggtattc gtgccctgat
     3241 ttcaggagac caacgggtca tgctcggcgc caaatcccaa gtccagatcg ataccccgga
     3301 ggtccgcgtc actgagtgga ggctcgcgcc gggcagcgcg accggccacc atattcacga
     3361 gatggactat gtgatcgtcc ccgtgacctc cggcgaaatg accatcgtgg cgcccaatgg
     3421 cgatcgctcc aaggcgcagc ttggggccgg aaaatcctac ttccgcaagg cgggcgtcga
     3481 gcacgatgtg ctcaacgaga cctcttcgga gatcgtgttt ctcgaggtcg aactcaaacc
     3541 gtaaaccatt cctttaccga attgccaccg atccgacgca gttgatccct caatgtgcct
     3601 caaagttccc ccatcagtgc gccgggagtg gccggcatgc tgaattacct gaagaaattc
     3661 gcgttggaca tcttgccgtc ggtggccgcc accatcatcg gcgcctacat cgtgaaccac
     3721 tacatcgcga ccaagcctga gaagccggcg gccgcggtgt ccgcggctgc aagcaaggcc
     3781 gatcccagga aagccgttgc caagaccgag ctgaagagcg aggcgaaggc cgatcccgcg
     3841 aagtcggcgc aagtcccggc tgagaccgcc agcatccccg agcccggcat caaggccaag
     3901 ggcatttccg agcgggtcat gatcgagaac cgcgcggctg aagggcccgc cgaggtcaag
     3961 ccggtcgaga agccggcgga cgtcaaggcc gtcgaaacca aacccgaagc caagcccgaa
     4021 gccaagcccg ccgagaccgc gagcgctccc gtcgagacgc gccg
//
LOCUS       DATLJT010001230         2701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4228309,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001230 DATLJT010000000
VERSION     DATLJT010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228309"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VM910_28335"
     CDS             complement(<1..642)
                     /locus_tag="VM910_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66456.1"
                     /translation="MQRVLREILGTLALAAIVVAASVDFAAAQKKYDTGVTDTDIKIG
                     NIMPYSGPASAYGIIGKTMSAYFRMINDNGGVNGRRINFITYDDAYSPPKTVEQARKL
                     VESDEVFLIFAPLGTASNAAIQKYMNNMRVPQLFVATGASRWGDPEHFPWTIGWQPNY
                     RAEGRVYATYILQHYPNAKLGVLYQNDDFGKDYVLGLKDVLRDKYERMVVASIS"
     gene            complement(1032..2051)
                     /gene="ilvC"
                     /locus_tag="VM910_28340"
     CDS             complement(1032..2051)
                     /gene="ilvC"
                     /locus_tag="VM910_28340"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVI66457.1"
                     /translation="MRVYYDRDADLNLIKGKKVVIVGYGSQGHAHALNLKDSGVKDVA
                     IALRKGSGSAKKAEAAGFKVFDVAEGAKWADLVMMLTPDELQGDIYREHLHDNMKKGA
                     ALVFAHGLNVHFNLLEPRADLDVLMIAPKGPGHTVRSEYQRGGGVPCLIAIARDVSGN
                     AHDLGLSYASAIGGGRAGIIETTFKEECETDLFGEQVVLCGGLVELIKGGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEFGEYVTGPRIITDETKKEMK
                     RVLADIQSGRFARDWMLENKVNQTSFKATRAKLAAHPIEEIGAKLRDMMPWIKKGALV
                     DKTKN"
     gene            complement(2204..>2701)
                     /locus_tag="VM910_28345"
     CDS             complement(2204..>2701)
                     /locus_tag="VM910_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVI66458.1"
                     /translation="FRRTLPHIAGITFGFAFMVAAVGFGFGTIFVAYPVLQTVLKYAG
                     AAYLVYLAFAIAVSGPPKPGEEGKRGPMTFWGAAMFQWVNVKGWVMVIGTITAYAAIA
                     RFPLNIAIQTVLCLALGVVATSVWTLFGSALRPILKSERAVRAFNVIMALLLLASLYP
                     VFMDA"
BASE COUNT          506 a          828 c          826 g          541 t
ORIGIN      
        1 ggaaatgctg gcaacgacca tgcgctcgta tttgtcgcgc agcacgtctt tcaggccgag
       61 aacatagtcc ttgccgaagt cgtcgttctg atagagcacg cccagcttgg cgttcggata
      121 atgctgcagg atgtaggtcg cgtagacacg accctccgcc cggtagttcg gctgccagcc
      181 tatggtccac gggaagtgct cgggatcgcc ccagcgcgag gcgcccgtcg cgacgaacag
      241 ttgcggcacc cgcatgttgt tcatgtactt ctggatcgcg gcattgctcg cggtgcccag
      301 cggcgcaaag atcaggaaga cctcgtcgct ctccaccagc ttgcgcgcct gctccaccgt
      361 cttcggaggg ctgtaggcat cgtcgtaggt gatgaagttg atccggcggc cgttgacgcc
      421 gccgttgtcg ttgatcatgc ggaaataggc gctcatcgtc ttgccgatga tgccgtaggc
      481 cgacgccggc ccgctatacg gcatgatgtt gccgatcttg atgtcggtgt cggtgacgcc
      541 ggtgtcgtat ttcttctgcg cggcagcaaa gtccacgctc gcggcgacga caattgcggc
      601 aagtgcaagt gtcccgagaa tttccctgag cacgcgctgc atgagttacc tcccctgcgg
      661 gccgagcgtg acggcgaaat cggacggttt cctcttcgtc tttttctgat tgaatgcgga
      721 cgatagcagg cagcgacctt cgccgaaagc agcgaggtat cgcccgatcg cgttctcctg
      781 cagcgcagca atcgagcgcg ccggcgattg ttgcgagacg aaactgggga atcagtctca
      841 ggactgacgt gtgcgcggtc tctcgccttc cgacagcttt tagcggcgat gagagagctt
      901 gagaggttgc cgctcttccc ggctgagatg gcttggcttt tcgggccggc aaggtcagcg
      961 cgaaatcgct ccggccaaga cgaccctctc cccatgcagg tccacaaggg gagagggtaa
     1021 gccgagctaa attagttctt cgtcttgtcg acgagcgcgc ccttcttgat ccacggcatc
     1081 atgtcgcgga gctttgcgcc gatctcctcg atcgggtgcg cggcgagctt ggcgcgggtc
     1141 gccttgaacg aggtctggtt gaccttgttc tccagcatcc agtcgcgggc gaagcggccg
     1201 gactggatgt cagccagaac ccgcttcatc tccttcttgg tctcgtcggt gatgatgcgc
     1261 gggccggtga cgtattcgcc aaattcggcg gtgttggaga tcgaatagtt catgttggcg
     1321 atgccgccct catagatcag atcgacgatc agcttcacct cgtgcaggca ctcgaaataa
     1381 gccatctcgg gggcatagcc ggcctccacc aacgtctcgt aaccgccctt gatcaactcg
     1441 accaggccgc cgcagagcac cacttgctcg ccgaacaggt cggtctcgca ctcctccttg
     1501 aaggtggtct cgatgatgcc ggcgcggccg ccgccgatcg ccgatgcgta gctcaagcct
     1561 agatcgtggg cgttgccgga aacatcccgg gcaatcgcga tcaggcaggg cacgccgccg
     1621 ccgcgctgat attccgagcg cacggtgtgg cccggtccct tcggtgcgat catcagcacg
     1681 tcgaggtcgg cgcgcggctc gagcaaattg aaatggacat tcaggccgtg ggcgaacacg
     1741 agcgccgcgc ccttcttcat gttgtcgtgc agatgctcgc gatagatgtc gccctgcagt
     1801 tcgtcaggcg tcagcatcat caccagatcg gcccacttgg cgccttcggc gacgtcgaac
     1861 accttgaagc cggcggcttc cgccttcttc gccgagcccg atcccttgcg cagcgcgatt
     1921 gccacatcct tcacgccgga atccttcagg ttcagcgcat gggcgtggcc ctggctgcca
     1981 tagccgacga tgacgacctt cttgcccttg atcaggttca aatcggcgtc gcgatcgtaa
     2041 taaacacgca tagtcgtttc ctcgctgagt ggcccaatcg gccgaaaatg aaagaatttg
     2101 ggggtggctt ggcaaatttc cggcgttgtc tagagcattt ttgtcatcct tggaaaccct
     2161 cttctcgcat ggcggccagt tcgcccgagg gacaccagca gcatcatgcg tccatgaaga
     2221 cgggatagag cgaagcgagc agcaaaagcg ccatgatcac gttgaaggcg cgcaccgccc
     2281 gttccgactt caggatcggt ctcagcgcgc tgccgaacag tgtccagacc gaggtcgcca
     2341 ccacgccgag cgccaggcac agcacggtct ggatcgcgat attgaggggg aagcgggcaa
     2401 tcgccgcgta ggcggtgatg gtgccaatta ccattaccca gcccttgacg ttgacccact
     2461 ggaacatggc cgcgccccaa aacgtcatgg ggccacgttt cccttcctcg cccggcttgg
     2521 gcggtccgga gaccgcgatg gcgaaggcca aataaaccag ataggcggca ccggcatatt
     2581 tcaggacggt ctgcagcacc ggataggcga cgaagatggt gccgaacccg aaacccaccg
     2641 ccgcgaccat gaaggcgaat ccgaacgtga tgccggcgat atgcggcaag gtgcggcgga
     2701 a
//
LOCUS       DATLJT010001231         4192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1243503,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001231 DATLJT010000000
VERSION     DATLJT010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243503"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VM910_28350"
     CDS             complement(<1..688)
                     /locus_tag="VM910_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI66459.1"
                     /translation="MADLEKFRKDTRAWLEANCPPEMRKPVATEDDTFWGGRNTKFSS
                     DAQRVWFERMRDKGWTVPDWPVEYGGGGLDGAETKVLREEMAAIGARSPLSSFGIWML
                     GPALLKYGTEAQKKEYLPKIAAGLIRWCQGYSEPNAGSDLASLQTRATSDGDDFVING
                     QKIWTSYANYADWIFCLVRTDPAAKKHDGISFILFDMASKGVSTKPILLISGRSPFCE
                     TFFDNVRVPKA"
     gene            832..1377
                     /locus_tag="VM910_28355"
     CDS             832..1377
                     /locus_tag="VM910_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI66460.1"
                     /translation="MRLKLLSPGEMSAEQKQTYDEAIAGKRGSAPPPMMAWLNSPEMA
                     RNATRLGETVRFNTIFPPKLSEIAILVTARHWGSHYEWFAHKRLALKGGMDSKIVDAI
                     RDRRTPSFDDPKGQVIYDVAKALHEGHGLPKALYDEAVNVLTLRGLVEVIGLCGYYTM
                     VSMTLNTFEFGLPDGQVSELT"
     gene            1482..1979
                     /locus_tag="VM910_28360"
     CDS             1482..1979
                     /locus_tag="VM910_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI66461.1"
                     /translation="MPQNPPIISGTRIGHVHLKVADLDRALSFYCGVLGFELMQRLSS
                     GAAFISAGGYHHHIGLNTWESKGGPPPPPGTTGLYHTAILYPTRPALADALHRVLSAG
                     IPLDGASDHGVSEALYLRDPDENGVELYWDRPKELWPRTADGKLAMFTRRLDLEDLLR
                     QREAS"
     gene            complement(1992..3320)
                     /locus_tag="VM910_28365"
     CDS             complement(1992..3320)
                     /locus_tag="VM910_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66462.1"
                     /translation="MSESSPIKVGDRHSAQRCRRTRDKNLHAPNHIAIDETALRYEGW
                     RIVAVCFLVATFGWAFGFYGQSVYLAELHREHGWPASLISGATTFFYLFGAALVVFVA
                     EVVKKFGPRNCLIAGVLSMAAAAAALGKVATPWQLYFANALLAFGWAGTSLAIITNTL
                     SLWFDQRRGMAISLALNGASFGGIAGVPLLVIAIGHFGFPTAMTVAASVMVALVVPVV
                     LIFVGQPPLHLGSPTATSMDAPSATEIRKHALRDIGFLSIAIAFALVLFAQVGFIVHL
                     IAFLDPVIGRASAAVAVALLTAMAVVGRVLFSTVIDRLNQRLASALSFVSQAAALLIL
                     INVRNEWLLIAACAVFGFSVGNLITLPALIVQREFAPKSFGVLISLITAINQITYAFG
                     PGVVGMLRDLSGSYTLPFYGCIALEVVAAVLIMFRGSASRLATPSSSPGR"
     gene            complement(3566..>4192)
                     /locus_tag="VM910_28370"
     CDS             complement(3566..>4192)
                     /locus_tag="VM910_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HVI66463.1"
                     /translation="DGRLDAEAFHRNHQPIWAVLQRYLDGKSGDVLETGSGTGQHAVH
                     FAAHSPGIVWWPSDLNERHLQSIAAWRAFSGLPNIKPPLKLDLADPDWSEATRAGGCP
                     PNLLAIFCANVIHIAPWRVAEGLFAGAGRMLQEGGYLFLYGPFKRNGKHTALSNAVFD
                     ASLRENNPDWGVRDIDDVEKFASSCGLALREVVEMPVNNLIVVFARSH"
BASE COUNT          836 a         1308 c         1349 g          699 t
ORIGIN      
        1 tggccttggg cacgcggaca ttgtcgaaga atgtttcgca aaacggcgag cggccggaga
       61 tgagaaggat cggcttggtg gagacgccct ttgaagccat gtcgaacaag atgaagctga
      121 tgccgtcatg cttcttggcc gcgggatcgg tgcgcaccag gcaaaagatc cagtccgcgt
      181 agttcgcata cgacgtccag atcttctggc cgttgatgac gaagtcgtcg ccatcgctgg
      241 tcgcgcgggt ctgcagcgag gcgaggtcgg agccggcgtt gggctcggaa taaccctggc
      301 accagcggat caacccggcc gcgatcttcg gcagatattc tttcttctgc gcctcggtgc
      361 cgtatttcag aagcgccggc ccgagcatcc agatgccgaa gctcgacagc ggcgagcgcg
      421 cgccgattgc ggccatctcc tcgcgcagaa cttttgtctc cgcaccgtcg agcccgccgc
      481 cgccatattc aaccggccag tccggcacgg tccagccctt gtcgcgcatg cgctcgaacc
      541 agacgcgctg ggcgtcggaa gagaatttgg tattgcgtcc gccccagaac gtatcgtctt
      601 cggtcgccac gggcttgcgc atctccggcg ggcaatttgc ttccaaccag gcgcgggtgt
      661 ctttgcggaa tttttcgaga tcggccatgg gttgcgtttc cactgtcaag atcgttgctt
      721 cgaacttagc ccggggattg cggatttcaa tatcctcgtg agcgggctac tctctctgcg
      781 gccatcggcc tgcgcagggt ggtaacaatt gaaaagaaag aggaaacagc aatgcgcctg
      841 aagttacttt cgcctggcga aatgagcgcg gagcaaaaac aaacctatga cgaggcgatc
      901 gccggcaaac gcggatccgc gccgccgccg atgatggcct ggctcaacag cccggagatg
      961 gcgcgaaacg ccacgcggct cggcgagacc gtgcgcttca acacgatctt tccaccaaaa
     1021 ctgtcggaga tcgcgatcct cgtcaccgcg cggcactggg gctcgcatta cgaatggttc
     1081 gcgcacaagc gcctcgcgct gaagggcggg atggactcga aaatcgtcga tgcgatccgc
     1141 gaccgccgca cgccgagctt cgacgatccc aagggacagg tgatctacga cgttgcgaag
     1201 gccctgcacg aggggcatgg ccttccgaag gcgctctacg acgaggcggt caatgtgctc
     1261 acactgcgcg ggctggtcga ggtgatcggg ctgtgcggct attacacgat ggtgtcgatg
     1321 acgctgaaca cgttcgagtt cggcttgccg gacgggcagg tgtcggagct tacgtgatcc
     1381 ctgccccact ctccccgttc cgacggggag agggttgggg cggggggcct caccacatat
     1441 atgctcgcgg atagtctcct caccggatcg acgaaagaac catgccgcaa aacccgccca
     1501 tcatatcagg cacgcgaatc gggcacgtcc atctcaaagt cgccgatctc gaccgcgcgc
     1561 tttcctttta ttgcggcgtg ctcggcttcg agctgatgca acgcctgagc tcgggcgcgg
     1621 ccttcatctc ggcgggcggc tatcaccacc acatcggtct caacacctgg gaaagcaagg
     1681 gcggccctcc gccgccgccc ggcaccaccg gcctctatca caccgcgatc ctctatccga
     1741 cccggccggc gctggcggac gcgctgcatc gcgtgctgag cgccggcatt ccgctcgacg
     1801 gcgccagcga tcacggcgtc agcgaggcac tgtacctgcg cgatcccgac gagaatggcg
     1861 tcgagctcta ttgggatcgg cccaaggaat tgtggccgcg cacggcggac ggcaagcttg
     1921 cgatgttcac gcgacggctc gacctcgagg atctcctgcg gcagcgtgaa gcgtcgtaac
     1981 tgagcgctgc ctcagcggcc cggcgaggaa gacggcgtag cgaggcggga agcgcttccg
     2041 cgaaacatga tcagcaccgc ggcaaccact tcgagcgcga tgcagccgta gaacggcagc
     2101 gtgtaactgc cggagagatc gcgcagcatg ccgaccacgc cgggcccgaa ggcataggtg
     2161 atctggttga tcgcagtgat caggctgatc agcacgccga acgatttcgg cgcaaactct
     2221 cgctgcacga tcagcgctgg cagcgtgatc agattgccga ccgagaagcc gaacaccgcg
     2281 caggccgcga tcagcagcca ttcgttgcgg acgttgatga ggatgagcag agccgcggcc
     2341 tggctgacaa aggacagcgc ggaagcaagc cgctggttga gccggtcgat cacggtggag
     2401 aacaggaccc ggccgaccac cgccatggcc gtgagcaacg cgaccgcgac cgcggcactg
     2461 gcacgtccga tcacgggatc gaggaacgcg atcagatgaa cgatgaagcc gacctgcgcg
     2521 aacagaacca gggcgaacgc gattgcaatc gacagaaagc cgatgtcgcg gagcgcatgt
     2581 ttgcggatct ccgtggcgga gggcgcgtcc atcgacgttg ccgtggggct ccccaggtgt
     2641 agcggcggct gtccgacaaa gatcagaacc acgggcacca caagcgcgac catgacggac
     2701 gctgcaacgg tcatagcggt cggaaaaccg aaatggccga tcgcaatcac gagcagcggc
     2761 acgccggcga taccgccaaa gctcgcgcca ttcagtgcaa gcgagatcgc catgccgcgc
     2821 cgctgatcga accagaggct cagcgtattg gtgatgatgg cgagacttgt gccggcccag
     2881 ccgaaggcga gcagcgcatt ggcgaaatag agttgccacg gcgttgcgac cttgccgagc
     2941 gccgccgcgg cagccgccat cgagagcacg cccgcgatca ggcagttgcg cgggccgaat
     3001 ttcttcacga cctccgccac gaacaccacg agcgccgcgc cgaacagata gaaaaaggtg
     3061 gtcgcgccgg agatcagcga ggcgggccag ccatgctcgc gatgcagttc ggcgagatag
     3121 acgctctggc cgtagaagcc gaacgcccag ccgaaggttg cgaccaggaa gcaaaccgcg
     3181 acgatccgcc agccctcgta gcggagcgcg gtttcatcga ttgcgatgtg gttcggggcg
     3241 tgcaagttct tgtctctggt tcttctgcat cgttgcgcgg aatgcctgtc cccgaccttg
     3301 atcggggatg actcggacat ccacccggtt cctgcgaacg ggttatcaag acaagctagg
     3361 gcgaaccgga agattggcca gatccgatcg cttcgacgca catcgaagtg tcagtagctg
     3421 tcatcagaag tccgcgcacc gcgcattagg tgctgagctc ggggaattcg tcgatcggct
     3481 cgagccgggc gcaccgcttg gtgaacgacg agaacgccgg gatgccaagc aaccggtaca
     3541 aacgggcgcg ggccatgcgt tggcctcaat gcgagcgcgc gaacacgacg atgagattgt
     3601 tgaccggcat ctcgacaact tcgcgcaagg ccaggccgca gcttgatgcg aacttctcga
     3661 catcgtcgat gtcgcgcacg ccccagtcgg gattgttctc ccgcaggctg gcgtcgaaca
     3721 cggcgttgga gagcgcggtg tgcttgccgt tgcgcttgaa agggccgtag aggaagaggt
     3781 acccgccctc ttgcagcatg cgtccggcgc cggcgaacag gccctcggcc acgcgccatg
     3841 gcgcgatgtg gatcacattg gcacagaaga tcgccaacag gtttggcgga cagccacccg
     3901 cccgcgtcgc ctcgctccaa tcgggatcgg cgagatcgag cttgagcggc ggcttgatgt
     3961 tcggcagtcc ggaaaaggcg cgccaggccg cgatgctctg cagatgccgt tcgttgagat
     4021 cgctcggcca ccaaacgatg cccggactgt gcgccgcaaa atgcaccgca tgctggccgg
     4081 tgccgctgcc ggtctccagc acgtcgccgg atttgccgtc aagataccgc tgcagcacgg
     4141 cccagatcgg ctgatggttg cgatgaaacg cctccgcatc aagccggccg tc
//
LOCUS       DATLJT010001232         5575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4228976,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001232 DATLJT010000000
VERSION     DATLJT010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4228976"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VM910_28375"
     CDS             complement(<1..373)
                     /locus_tag="VM910_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HVI66464.1"
                     /translation="MNSSKKPYRIAVIPGDGIGKEVMPEGLRVIEAAAKRHGVAVQFD
                     HFDFASYDYYEKHGQMMPDDWKQKIGKHDAIYFGAVGWPAKIPDHISLWGSLIKFRRE
                     FDQYVNLRPVRLMPGVPSPLAN"
     gene            608..1384
                     /locus_tag="VM910_28380"
     CDS             608..1384
                     /locus_tag="VM910_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66465.1"
                     /translation="MDLHLRGKRVLITGASKGIGAAAAESFAEEGCHLRLAARSGDRL
                     KALAERLRSQHQIDATIHITDLRKSEDIQRLVKEASDIEILVNNAGDIPGGSIDKIDE
                     PTWRHAWELKVFGYVNLTRAIYAQMKARGGGVIVNDIGAAGEKFDANYICGSAGNAAL
                     MAFTRALGGKSLADNIRVVGINPGPVGTDRHVTLLKTRAKHQFGDESRYKEFQKGMPL
                     GRPAHAREIGDLMAFLASDRSGYTSGVIFTVDGGLAAGWG"
     gene            complement(1412..2263)
                     /locus_tag="VM910_28385"
     CDS             complement(1412..2263)
                     /locus_tag="VM910_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66466.1"
                     /translation="MSTPSDAPDWRKLSRQELDRGLNNGDAVKNSDEIVASWDAASAE
                     LRKRHPDHLDLRYGPRDRNRIDFLPARKNAPTLLFIHGGYWQRRAKENFSLFAEGPMA
                     YGINVALIGYTLAPEATLDQIVAEIHAGIDFLVAQLPTLGGDDKGIVVSGWSAGGHLT
                     AMALSHAKVRAGLGISGIYDLEPIRHSYLNVKLSLDEVASRRNSPMMQAGGPTKPLSL
                     VVGSAELPLLRRQTSDFAGHRAKYGLPVTYEEIPGADHFSVMNEMLAPMGRITTLIRQ
                     LFARTDG"
     gene            2359..3783
                     /locus_tag="VM910_28390"
     CDS             2359..3783
                     /locus_tag="VM910_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI66467.1"
                     /translation="MADQGLVRETACAIVDRLKRGEITPLDLLDALEERIGEVDTKVN
                     ALPILCFDRARTHARALMQKPAGERGLLAGLPVPIKDLFDVKGVRNTQGSPIFKDRIC
                     EQSDIVVETLEANGAVVYAKSNTPEFGAGAHTFNEVFGATLNPWDTSKSAAGSSGGAA
                     VALATGMAWLAHGTDMGGSCRNPASFCGVVGLRPSIGRVAHSPKFKIDRNLTVHGPMA
                     RNVEDLALLLDAMSGEHPADPLSLPRLATSFLSAARDGKNPKRVAYSPDLGITPVDPE
                     VVSVTRKAAQRFADAGAIVEEAHPDLREAHECFHVLRAFDFAISKAALLRKHRDLLKP
                     EVIWNIEQGLKLTVEQLERAEAQRMTLAERALKFFETYDLLLSPATIVPPFPVQDRYV
                     AECAGKTFDNYVEWLGIVYAITLACCPALSLPCGFTASGLPVGLQMVARPRAEAELLA
                     GAKVLEDILGLRDTTPIDPRPARI"
     gene            complement(3896..4534)
                     /locus_tag="VM910_28395"
     CDS             complement(3896..4534)
                     /locus_tag="VM910_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI66468.1"
                     /translation="MSPLQRKPVNTYHHGDLREALVQAALREAEREGPEAISIKGLAK
                     KLGVSQPAPYRHFADREALLQAVTAEAFRQFNAVLREAIRKPSKRSKLSRFASAALDF
                     GLRRNGIYRLMFASRTMACATTDSELHMAAKEAFALLVESFEAPAVGLLRERHALQIW
                     VALHGMITLAEQGLITGHAAQVTREELIEDIVQQTKMALAAAIVTAGKQGQG"
     gene            4699..4908
                     /locus_tag="VM910_28400"
     CDS             4699..4908
                     /locus_tag="VM910_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HVI66469.1"
                     /translation="MAKKLKIHVDQDKCQGHARCKSLAPELFELDEYGNAHEVGDGSV
                     PEGLEDKAWLAQTNCPEIAIEVTEE"
     gene            4964..>5575
                     /locus_tag="VM910_28405"
     CDS             4964..>5575
                     /locus_tag="VM910_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI66470.1"
                     /translation="MSDSAPVMPEHPPVTDWVHDFDHTDPRWTENPYPIWNELRAASP
                     VVHTERFLGCYMPTTYEAVREIAHDTHHFSSRRVIVRDVRPEVTNPAPPITSDPPEHK
                     PAKQLLLPPFTPDAMKKLEPRVRAICNELIDAFIADGKCDAAARYTKHIPVRAIAHML
                     GIPEKDGDLFIKWIHEILELGIKDDDTLMRAVHEMTAYFAGHIE"
BASE COUNT         1082 a         1825 c         1676 g          992 t
ORIGIN      
        1 ggttggcaag cggcgacggc acacccggca tcagccgaac cgggcgaagg ttgacgtatt
       61 gatcgaactc gcgccggaac ttgatcagcg agccccacag ggagatgtga tcgggaatct
      121 tggcgggcca gccgaccgcg ccgaaataga tcgcgtcgtg cttgccgatc ttctgcttcc
      181 agtcgtccgg catcatctgg ccgtgctttt cataatagtc gtaggaggcg aaatcgaagt
      241 gatcgaactg cacggcgacg ccgtgccttt tcgccgccgc ctcgatgacg cgcagaccct
      301 cgggcatgac ttccttgccg atgccgtcgc cggggatgac cgcgatccgg tatggtttct
      361 ttgagctgtt catcggggaa ttccttgtcg tgccggcctt tagtcccgct actgatccga
      421 ttttgaggtt cgccaaggga attggcggca gatggtggcg aacctcaatc cgactggcgt
      481 gatcgccctg caatggacca aaccgggagt ttgcgcaacg ctgcagtgca atgttgaccg
      541 tggctcggcc tgccgcctag ttttttcgat ccctcatcgt tcccctgccc aaccagcgag
      601 aactcccatg gacctgcatc tgcgcggcaa gcgtgttttg atcactggcg cgtcgaaagg
      661 catcggcgcg gccgcggccg aatcctttgc cgaggaaggc tgtcacttga ggctcgccgc
      721 gcgcagcggc gatcggttga aggcgctggc ggagcgcctg cgctcgcagc accagatcga
      781 cgccaccatc cacatcaccg atttgcgcaa gagcgaagac attcaacggc tggtgaagga
      841 agcctccgac atcgagatcc tcgtcaacaa tgccggcgac attcccggcg gatcgatcga
      901 caagatcgac gaaccgacct ggcgccacgc ctgggaactg aaggtgttcg ggtatgtcaa
      961 cctgacgcgg gcgatctacg cccagatgaa agcgcgcggc ggcggcgtga tcgtcaacga
     1021 catcggcgcg gccggcgaaa aattcgatgc caactacatc tgcggcagcg ccggcaacgc
     1081 agcactgatg gcgttcaccc gcgcattggg cggaaaaagc ctcgccgaca atatccgcgt
     1141 ggtcggcatc aatcccggcc cggtcggcac cgaccgccat gtcactctgt tgaagactcg
     1201 cgcgaaacat cagttcggtg acgagagccg ctacaaggag ttccagaaag gcatgccgct
     1261 cggccggccc gcccatgcca gggagatcgg cgatctcatg gccttcctcg cctccgaccg
     1321 ctccggctat acctcgggtg tgatcttcac cgtggatggc ggcctcgccg cgggttgggg
     1381 ataaagggct ccgtcgcccg tggaacgacg ttcacccatc cgtccgcgca aacaactgcc
     1441 ggatcagcgt cgtgatccgg cccatcggcg caagcatctc attcatgacc gaaaaatggt
     1501 cggcgcccgg aatctcttca taggtcaccg gcaggccata tttcgcacga tggccggcaa
     1561 aatccgaggt ctgcctgcgc agcagcggca gttccgccga accgactacc agcgacagcg
     1621 gcttcgtcgg ccctcccgct tgcatcatcg gcgagttgcg gcgcgaggcc acctcgtcga
     1681 gagagagctt gacgttgaga taggaatggc ggatcggctc gagatcgtag atgccgctga
     1741 tgccgagccc tgcccttacc ttcgcatgcg acagcgccat cgcggtgaga tggccgcccg
     1801 ccgaccagcc ggaaaccacg atacctttgt cgtcgccgcc aagcgtcggc aattgcgcaa
     1861 ccagaaaatc gatgccggca tgaatctcgg caacgatctg atcgagcgtc gcctcgggag
     1921 cgagcgtata gccgatcagc gcgacattga tgccgtatgc catcggacct tcggcaaaca
     1981 gcgaaaagtt ctctttcgcc cggcgctgcc agtagccgcc gtggatgaac agcagcgtcg
     2041 gcgcgttctt gcgcgctggc aaaaaatcga tgcgattgcg atcgcgcggg ccatatcgga
     2101 gatcgagatg gtcgggatga cgcttgcgca gttcggcaga agcggcgtcc cagctcgcaa
     2161 cgatctcgtc gctattcttg accgcatcgc cgttgttgag gccgcggtcc agctcctgcc
     2221 ggctcaattt tcgccagtcg ggtgcatctg acggcgtcga catttggaaa cctccgcgct
     2281 cgttgccacc gggtccgaaa atgttctaca gcatgacgca actcgccgcc acgacgcgaa
     2341 acaaatggag agacggcatt ggctgaccag ggactggtgc gggaaaccgc ttgcgcaatc
     2401 gtcgacaggc tcaagcgcgg cgagatcacg ccgctcgatc tgctcgacgc gctggaagag
     2461 cggatcggcg aggtcgatac gaaggtcaac gcactgccga tcctctgctt cgaccgcgcc
     2521 cggactcacg ccagagccct gatgcaaaaa ccggccggcg agcgcggact gctcgcgggc
     2581 ctcccggtgc cgatcaagga cctgttcgac gtcaagggcg tgcgcaacac gcagggctca
     2641 ccgatcttca aggacaggat ttgcgagcag tcggacatcg tcgtcgagac cctcgaagcc
     2701 aacggcgcgg tcgtctacgc caagtcaaac acgccggaat tcggcgcggg cgcgcacacc
     2761 ttcaacgagg tgtttggcgc cacgctcaat ccgtgggaca cctccaaatc cgctgccggc
     2821 tcttcgggcg gcgcggccgt cgcgctcgcg accggcatgg cctggctcgc gcacggcacc
     2881 gacatgggtg gctcctgccg caatcccgcg agcttttgcg gcgtcgtcgg cctgcggccg
     2941 agcatcggcc gggtcgcgca ttcgccgaag ttcaaaatcg accgcaatct caccgttcac
     3001 ggaccgatgg cgcgcaacgt cgaggacctc gcgctgttgc tcgatgcgat gagcggcgag
     3061 catccggccg acccgctgtc gttgccacgg ctcgcaacct cgttcctttc cgcagcccgc
     3121 gacggcaaaa atccgaagcg cgttgcctat tcgccggatc tcggcatcac cccggttgat
     3181 cccgaggtcg tcagcgtcac gcgcaaagcg gcgcagcgct ttgccgacgc cggcgcaatc
     3241 gtcgaggaag ctcatccgga cttgcgcgaa gcccatgaat gcttccacgt gttgcgcgcg
     3301 ttcgatttcg ccatcagcaa ggccgccctg ttgcgcaagc atcgcgatct gctcaagccc
     3361 gaggtgatct ggaacatcga acaagggctc aagctcacgg tcgagcagct cgagcgcgcc
     3421 gaggcgcagc gcatgacact cgccgagcgc gcgctgaaat tcttcgagac ctacgatctc
     3481 ctgctgtcgc ccgcgaccat cgtccctccc tttcctgtcc aggaccgcta tgtcgccgag
     3541 tgcgccggaa agacattcga caattacgtc gaatggctcg gcatcgtcta cgcgatcacg
     3601 ctcgcctgtt gcccggcgct gtcgctaccc tgcggcttca ctgcttccgg cctgccggtc
     3661 ggcctgcaaa tggtggcgcg gccgcgcgcc gaggccgagc ttctggcggg cgcaaaagtg
     3721 ctggaggata tcttgggctt gcgcgacacg accccgatcg atccgaggcc ggcaagaatt
     3781 taggcctccc ggtgaggagt tccgggcgca gccgtagcgt tcgtggcgga tgaaagttgc
     3841 ttccgcatgt cggcggagaa gatgcgcctc tcccagatac ggatcgagat cgcggctagc
     3901 cttgaccctg tttgcccgcg gtcacgatcg cggccgccag cgccatcttg gtctgctgca
     3961 cgatgtcctc gatcaattcc tcgcgagtga cctgcgccgc gtgaccggta attagcccct
     4021 gctccgccag tgtgatcatc ccgtgcagcg ccacccagat ttgcagcgcg tggcgctcgc
     4081 gcaacaatcc aacggccggc gcctcaaacg attccaccag cagcgcaaag gcttcctttg
     4141 ccgccatgtg aagctcgcta tccgttgtcg cacaggccat ggttcgcgat gcgaacatca
     4201 gccgataaat cccgttccgc cgcaggccga aatcgagcgc ggctgaggcg aaacgcgaca
     4261 gtttcgagcg tttcgacggt tttcgaattg cctcgcgcaa gaccgcgttg aattgccgga
     4321 aggcttcggc cgtcaccgcc tgcaacagcg cctcgcggtc ggcgaaatgc cggtagggcg
     4381 ccggctgcga cacgccgagc tttttcgcga ggcccttgat gctgatagct tcgggcccct
     4441 cgcgctcggc ttcccgcagg gcagcctgca cgagcgcttc cctgaggtcc ccatggtgat
     4501 aggtattgac cggtttgcgc tgcaggggcg acatcgggag cgacaatctt ttctcttgcg
     4561 gccgggcgcg atgctaatgt aatagactat aacttaaggc tcggtcaacg agccgtcaaa
     4621 ggaaattgcg ccgaaacaag aaaacgaggc gcgcaggcct caagagtttg gccgtcagct
     4681 cagggagaag ccgtcgcgat ggcaaaaaag ctgaaaatcc acgtcgacca ggacaaatgc
     4741 caggggcacg ctcgctgcaa atcgctcgca cccgaattgt tcgagctcga cgaatacggg
     4801 aacgcccatg aagtgggcga cggctcggta cccgaaggcc tggaagacaa ggcctggctc
     4861 gcccagacca attgcccgga aattgcgatc gaggtcaccg aggagtgagt tccccggcgg
     4921 ccagcctcga acctcacgaa caaaaccaga ggattttgcg ctgatgtccg actctgcccc
     4981 tgtcatgccc gaacacccgc ccgtcaccga ttgggtgcac gatttcgacc acaccgatcc
     5041 gcgctggacg gagaacccgt atccgatctg gaacgaactg agggccgcct cgcccgtggt
     5101 tcacacagaa cgcttcctcg gctgctacat gcccacgacc tatgaggcgg tgcgcgagat
     5161 cgcccacgac acacaccatt tctcctcgcg ccgcgtgatc gtgcgcgacg tcagaccgga
     5221 ggtcaccaac cccgccccgc cgatcacctc cgatccgccg gagcacaagc cggccaagca
     5281 attgctgctg cccccattca cgcccgacgc gatgaagaag ctggaacctc gggtccgcgc
     5341 gatctgcaac gagctgatcg acgcgttcat cgccgacggc aaatgcgacg ccgccgcgcg
     5401 ctacaccaag cacattccgg ttcgcgccat cgctcacatg ctcggcatcc ccgaaaagga
     5461 cggcgacctc ttcatcaaat ggattcacga gatcctcgag ctcggtatca aggacgatga
     5521 cacgctgatg cgggcggtcc acgagatgac cgcctatttt gccggccata tcgag
//
LOCUS       DATLJT010001233         1893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4229086,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001233 DATLJT010000000
VERSION     DATLJT010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4229086"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VM910_28410"
     CDS             <1..1237
                     /locus_tag="VM910_28410"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI66471.1"
                     /translation="LAAAAPEQVGMSGERLGRITTMLKKEIADGKLPGAVVLVARKGK
                     IVYSDAMGSQDKGANAPMQLNSIFRIYSMTKPLASAAAMMLVEDGVIQLTDPVSKFLP
                     AFKDMQVSVATIGADGKTTYANVPATRPIIVQDLLRHSAGLAYAEITKNEPVKAAYVE
                     AKFSQPGVHEYDSRGMTPAEQVARIAKAPLIHQPGTTWEYSMAVDILGRVVEAASGKR
                     LAVVLDERLFKPLKMVDSSFWLPPEKMSRLAQPLPVDPASGQKISVLDVSAEPMNDSA
                     GAGALSTATDYLRFAQMLLNGGELDGVRVLSRSTIKLMTSDHLGTRIAAPFQPGELLL
                     GTPGYTFGLGFAVRQGDGVAGVAGSAGEFMWAGYAGTYFWVDPKEEIVGIYMSQAPSP
                     IRAYYRKMFKGLVYQALVD"
     gene            1403..>1893
                     /locus_tag="VM910_28415"
     CDS             1403..>1893
                     /locus_tag="VM910_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66472.1"
                     /translation="MRTKIEATWIVGYARGHHTLIRDGELVFEGNKILHAGKAFEGKV
                     DRVIDAKGKLVSPGFIDTHVHSGHRASHRLITDTGRPMYYGQPFLEISVPKEGKVVKG
                     DPRYLKHGDAGSAAAFELNAAFTVAELLRNGVTTFIEYGSQLNVQDALLAEVVRLGSR
                     AYLA"
BASE COUNT          363 a          604 c          592 g          334 t
ORIGIN      
        1 cctcgccgcg gcagcgcctg aacaggtggg catgtcaggg gagcgccttg ggcgcatcac
       61 cacgatgctc aaaaaggaga ttgccgacgg taagttgccg ggggcggtgg tcctggtcgc
      121 ccgcaagggg aagatcgtct attcggacgc catgggctcc caagacaagg gcgcaaacgc
      181 gcctatgcag ctcaattcga tcttccgcat ctactcgatg acgaagccgc ttgcctcggc
      241 agccgcgatg atgctggtcg aagacggcgt catccagctc accgatccag tctccaagtt
      301 tctgccggcg ttcaaagaca tgcaggtgag cgtggcaacc atcggcgccg acggcaagac
      361 cacctacgcg aatgtgccgg cgacaaggcc gatcatcgta caggaccttc tgcgtcacag
      421 tgcgggcctc gcctatgcgg agatcaccaa gaacgagccg gtgaaagcgg cctacgttga
      481 ggccaagttc tcccagcccg gcgtccacga atacgactcg cgcggcatga cgccggccga
      541 gcaagtcgcg cgcatcgcca aggcgccgct cattcaccag cccggcacga cgtgggagta
      601 cagcatggcc gtcgatattc tcggccgagt cgtcgaggca gcgtcgggca agcggcttgc
      661 cgttgtcttg gatgagcggt tgttcaaacc gctcaagatg gtggactcga gtttctggct
      721 gccgccggag aagatgtcgc ggctcgcgca gccgctgccg gtcgatccgg cctccggcca
      781 gaagatcagc gtgctcgacg tgtcggcaga gccgatgaac gattccgctg gcgccggcgc
      841 gctttcgacc gccaccgatt atttgcggtt cgcacagatg cttctgaacg gcggcgagct
      901 cgatggtgtg cgcgtgctga gccgctccac catcaaactg atgacgtcgg accatcttgg
      961 cacacgcatc gcagcgccgt tccagccggg tgagcttctg ctggggaccc ccggctatac
     1021 gttcgggctc ggctttgccg tgcgccaggg cgacggggtt gccggcgtgg cgggctctgc
     1081 gggcgaattc atgtgggctg gttacgccgg cacctatttc tgggtcgatc ccaaggaaga
     1141 gatcgtcggt atctacatga gccaggcgcc aagcccgatt cgcgcatatt accgcaagat
     1201 gttcaaaggg ctcgtctatc aggcgctcgt ggactgaaag ccactcgcac tgcaaacgtg
     1261 cgaggccgca gcgtctcgcg gcctcgctct tcaacattcg aaggctcggg agttctctcg
     1321 caacctcgag agcgccacag cgctcggatt gccgggtgac atagcagctc taacgtcgcc
     1381 agctcggtta agtggagaca gcatgcgcac caaaatcgag gcgacgtgga tcgtgggcta
     1441 cgcgcgcggg catcacaccc tcatccgcga cggagaactc gtgttcgagg gcaacaagat
     1501 cctccacgcc ggcaaagcat tcgaaggcaa ggtcgacagg gtcatcgatg ccaagggcaa
     1561 gctggtctcg cccggcttca tcgacaccca cgtccattcc ggccaccgcg cctcgcatcg
     1621 actgatcacc gataccggcc ggccgatgta ttacggtcag ccattcctgg aaatttccgt
     1681 tcccaaggaa ggcaaggtcg tcaaaggcga cccacgctac ctcaagcatg gcgacgccgg
     1741 ctcggcggcc gccttcgagc tcaacgccgc attcactgtg gccgagctcc tgcgcaacgg
     1801 ggtgacgacc ttcatcgaat acggcagcca gctcaatgtg caggatgcgc tgctggccga
     1861 ggtcgtgcgc ctcggcagcc gcgcctatct ggc
//
LOCUS       DATLJT010001234         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4001091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001234 DATLJT010000000
VERSION     DATLJT010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4001091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VM910_28420"
     CDS             <1..871
                     /locus_tag="VM910_28420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVI66473.1"
                     /translation="VTSLAEEIGERNVFNSEALRRAAVYIEAEWDALGYGVERLEYDV
                     SGIRCANLIATREGNTRRNEILLLGAHYDSVVGSPGANDNASGVAALLETSRMFQALE
                     PALTVRFVAFVNEEPPFFRTDQQGSMVYAQAARRRGDDIRLMASLETIGCYSSKPGSQ
                     RYPPLFRFFYPDRGNFIGIVSDFRSSAAMRRLASAFRAHSDFPMQTASTFRFVPGVAW
                     SDHDSFWRQGYFAVMVTDTAFYRYRHYHASTDTPDKLSYPEFANVTLGLFRAFAVLAS
                     EGVNPSSPQKLNA"
     gene            1148..1312
                     /locus_tag="VM910_28425"
     CDS             1148..1312
                     /locus_tag="VM910_28425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66474.1"
                     /translation="MDYKLFDELKKAQEARIKQEQRGEVITKLLNEANKQSEKTDVAE
                     TPIKEVAPAK"
     gene            complement(1326..2261)
                     /locus_tag="VM910_28430"
     CDS             complement(1326..2261)
                     /locus_tag="VM910_28430"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HVI66475.1"
                     /translation="MDSRSLLAPSREKERSKARPERRPLLVIDGDSFAHRSYHALPKT
                     IRRDGRRPAGAILGFANRLLQLYRTERPRAVLVGWDTLEVGTYRHKAFPAYQSGRDFD
                     DELVEQLELIPKFVAACGFANARRAGYEADDFLAAAAAAEERRGGTVLVASGDRDTFQ
                     LASEKTTILYPVKAGEMARIGPKEVGERYGVAPKQVPDFIALRGDPSDRLPGAPGIGA
                     HGAAALIRQYGSLAALLKAGRFAKQAEDLKLYRSIATMDKKAPLPTIAAQTPTWHKAA
                     ALARKWQLNKLAGRLEEMARKTSPPSARSIETELG"
     gene            complement(2307..2601)
                     /locus_tag="VM910_28435"
                     /pseudo
     CDS             complement(2307..2601)
                     /locus_tag="VM910_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
BASE COUNT          480 a          797 c          824 g          500 t
ORIGIN      
        1 tgtcacatcg cttgccgaag aaattggcga gcgcaatgtt ttcaactccg aggcgcttcg
       61 acgcgcggca gtctatatcg aggctgaatg ggacgctctg ggctatgggg tagagcggct
      121 cgagtacgac gtttccggaa tccgctgcgc aaaccttatc gccacacgtg aagggaacac
      181 acgtcgcaac gagatcttgc tactgggcgc gcactatgac tcggtcgttg gcagtccggg
      241 agccaatgac aatgccagcg gcgttgccgc gctgttggaa acatccagaa tgttccaggc
      301 gcttgaaccg gcgctcacag tccgctttgt cgccttcgtc aatgaagagc cgccattttt
      361 tcggactgac cagcagggaa gcatggttta tgcccaggcg gcccggcgtc ggggtgacga
      421 catccggctg atggcatcgc ttgagaccat cgggtgctac agcagcaaac caggcagtca
      481 gcgttacccg ccgttgttcc ggtttttcta tcccgaccgc ggcaacttca tcggaatcgt
      541 atcggacttt cgctccagtg cggcgatgcg gcggcttgct tccgcgtttc gcgcccactc
      601 cgacttccct atgcagactg cttcgacgtt ccggttcgtt cccggagtgg catggagcga
      661 ccacgattca ttctggcgcc aaggctactt tgccgtgatg gtcacggaca cggcgttcta
      721 ccgctaccgg cactaccacg cttccaccga tacgcccgac aagctttcct atccggagtt
      781 cgcaaacgtt acgcttggcc tgttcagggc gttcgcagtc ctcgccagtg agggggtaaa
      841 tccctcctca ccccagaaac tgaatgcgta aatgtgacca tcggcgccga catacagacg
      901 gtctggcgcg gacctgcggt gaatgatctc aatctccaag ccaggtagac gcgcgctcgc
      961 gtgcgtcgtg tcgtcttcga tttgctggtt cgacaagcat ttctccatct agcgaacccc
     1021 aagactcaat ccccaaagcg atcaacggtt agtgagtgag gctcgcaatg ggcttggaat
     1081 tatggtctga tttacagcgt tcgttcccct ttgttcatca cgaatggagg agttaatgtg
     1141 ctgggaaatg gactacaagc tcttcgacga attgaagaag gcgcaggaag ctcggattaa
     1201 gcaagagcag cgcggtgaag tcatcaccaa gcttctgaac gaagccaata aacaaagcga
     1261 aaagacggac gtcgcagaaa cgccgatcaa agaggtcgca cccgcgaaat agcctagcgt
     1321 tgcgattagc ctagttcggt ttcgatagag cgtgcggagg ggggagaggt tttcctcgcc
     1381 atctcctcga ggcggccggc gagcttgttg agttgccatt tgcgggcgag cgcagcggcc
     1441 ttgtgccagg tcggtgtctg cgccgcgatc gtcggcagcg gcgctttctt gtccatggtg
     1501 gcgatggagc ggtaaagctt gagatcctcg gcctgcttgg cgaagcgtcc cgctttcagc
     1561 agcgcggcga gactgccgta ttggcgaatc agcgcagcgg caccgtgcgc gccgatgcca
     1621 ggcgcgcccg ggagtcggtc cgacggatcg ccgcgcagcg cgataaaatc cggcacctgc
     1681 ttgggcgcga ctccgtagcg ctcgcccacc tccttcggcc cgatgcgcgc catttcgccg
     1741 gcctttaccg ggtagaggat cgtggttttt tccgatgcga gctggaaggt gtcgcggtcg
     1801 ccgctggcaa cgagcacggt gcccccgcgt ctctcctcgg ccgccgccgc tgcggcgaga
     1861 aaatcgtcgg cctcgtagcc agcgcgccgg gcattggcga agccgcaggc ggcgacaaat
     1921 ttcgggatca gttcaagctg ctcgacgagc tcgtcgtcga agtcgcggcc gctctgatag
     1981 gccggaaaag ccttgtgccg gtaggtcccc acttcgagcg tatcccagcc gaccagcacc
     2041 gcgcgcggcc gctcggtgcg atagagctgc agcaagcgat tggcgaaacc gaggatcgcg
     2101 cccgccggcc gcctgccatc gcggcggatc gtcttcggca gcgcatgata ggagcggtgc
     2161 gcgaaggaat cgccgtcgat caccagcaaa ggacgacgct cgggtctagc cttgctccgc
     2221 tccttctcac gcgatggggc gagcagtgag cgggaatcca cagcgggtcc gcgctcgcgc
     2281 ggggaggaac ggccgccggc tgcttgcttc aactctaccc aggctggcgc gtggtcgctg
     2341 gcgccggctt ctccgcgcac cttgcggtcg acgccggcgg cggcgagccg cttcgctgcc
     2401 agcgagctca acagcagatg atcgatgcgc agcccggcat cgcgcggcca gcggttccga
     2461 agatagctcc aataggtgta gagcgcggcg tcgggatgca gcgcacggag ggcgtcgcgc
     2521 cagccctgcc tgagcagttt ctgaaactgc gcgcgcggcg ccggctgcag cagcgcgtcg
     2581 tcggcatagg agcgcaccgc g
//
LOCUS       DATLJT010001235         1911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2852331,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001235 DATLJT010000000
VERSION     DATLJT010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852331"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VM910_28440"
     CDS             complement(<1..960)
                     /locus_tag="VM910_28440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66476.1"
                     /translation="MLFAFSGRATRNFLVLVVVMHFILFVDRVNLAAAASVMQQDLGL
                     SNIALGIAFSAFNYAYAPFQLIGGWFADRFGARRTLTACGLTWSITTIVTGAVTGLVS
                     LFAVRLVLGMGEGATLPAVTRALSKWTSLAARGTAVGITHAAGRLGAGASAPIVALLI
                     TWFSWRFSFVALGVVSAFWAAAWWWFFHEDPRQHPRITRAELAALPAADPAGRIGSGP
                     VPWRRLVPRVAPLMIVYFCQGWTGWLFVTWMPSLLQKNYGLDLKKSSFLYAAMLFCAM
                     IAELLGGVVTDYLLRRTENLQIARSRMIAVSWVLVVAGLVPAIL"
     gene            complement(1005..>1911)
                     /locus_tag="VM910_28445"
     CDS             complement(1005..>1911)
                     /locus_tag="VM910_28445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66477.1"
                     /translation="GHRFLIDQLLTDTGSGHNITQTVFIECGSMYRADGPVEMKPVGE
                     TEFVNGTAAMSASGQYGPTRLCRGIVGHADLRLGDAVARVLEAQIAAGDGRFRGIRHS
                     VTWDPSDLLSRGRTNSLKGQMYDPAWRTGFARLAPLNLTFEAWLYHPQLADLADLARA
                     FPQTTIILNHVGGPVGIGPYKDKKAETFAQWKAGITEVAKLQNVVIKLGGLGMTFGMF
                     DFYTREKPPSSTDLEAAYRPYIETCIEAFGVNRSMFESNFPPDGASSSYPILWNAFKR
                     LAAGSSAYEKAMLFNGTAKRVYRLA"
BASE COUNT          398 a          614 c          604 g          295 t
ORIGIN      
        1 gaggatcgcc ggcaccagcc ccgccacgac gaggacccag ctgaccgcga tcatccgcga
       61 gcgcgcgatc tgcaggttct cggtacggcg caacaaatag tcggtcacga cgccgccaag
      121 caactcggca atcatcgcgc agaacagcat cgccgcgtac aagaacgagg acttcttcag
      181 atcgagcccg tagttcttct gtagcaatga cggcatccag gtcacaaaca gccagcctgt
      241 ccagccctgg cagaaataga caatcatcaa cggcgccacg cgggggacga ggcgccgcca
      301 cggcaccgga ccggaaccga tccgccccgc gggatcagcg gccggtaatg cggccagttc
      361 cgcacgggtg atgcgggggt gctgacgcgg atcctcatgg aagaaccacc accacgcggc
      421 ggcccaaaaa gccgacacga cgccgagtgc cacaaacgaa aaacgccagg agaaccaagt
      481 gatcagcaag gcgacgatcg gcgccgacgc ccccgcgccg aggcgcccgg cggcgtgggt
      541 gatgccgacc gcggtgccgc gcgcggcgag cgaggtccat ttcgacagcg cacgggtgac
      601 cgccggcaac gtcgcaccct cgcccatgcc gagcaccagc cgcaccgcaa acagcgaaac
      661 gagcccggta accgccccgg tcacgatcgt cgtgatagac caggtcagtc cacaggcagt
      721 caaagtgcgc cgcgcgccga agcggtcggc aaaccagcct ccgatcagct gaaacggagc
      781 ataggcgtaa ttgaaggccg agaaggcgat gccgagcgcg atgttgctga ggccaaggtc
      841 ctgctgcatc acgctcgccg ccgcggccag gttgacccgg tcgacaaaga ggatgaagtg
      901 catcacgacc acgaggacca ggaaattcct ggtggcgcgt ccgctgaagg cgaacagcat
      961 gttggcctcc tttcgctctc gtttcttctg ggacgcttcg agcctcaggc cagccggtag
     1021 acgcgttttg cggtgccgtt gaacagcatt gccttctcgt aggccgagga cccggcggcc
     1081 aggcgtttga aagcgttcca taggatcgga tagctcgagc tcgctccgtc cggcgggaag
     1141 ttgctctcga acatcgaacg attcacgccg aacgcctcaa tacaggtttc gatgtagggc
     1201 ctgtaagccg cctcaaggtc agtcgaggaa ggcggcttct cgcgcgtata gaaatcgaac
     1261 atgccgaacg tcatgccgag cccgccgagc ttgatgacga cgttttgcaa cttagcgacc
     1321 tcggtgatcc ccgccttcca ttgcgcaaag gtctcggcct tcttgtcctt gtacggcccg
     1381 atgccgaccg gtccgccgac atggttgagg atgatcgtgg tttgcgggaa ggcgcgggcg
     1441 aggtcggcga gatcggcaag ctgaggatgg taaagccaag cctcaaaggt gaggttcaag
     1501 ggtgcgagac gggcgaagcc ggtgcgccag gcggggtcgt acatctgccc cttcagcgaa
     1561 ttggtacgcc ccctggacag caaatcgctc ggatcccagg tcacggaatg gcggatgccg
     1621 cgaaagcggc cgtctcctgc ggcgatctgg gcctccagca cgcgcgcgac tgcgtcacct
     1681 aatcgcaaat cggcatgacc gacgatgccg cgacagagcc gcgtcggccc atattggccg
     1741 ctcgcgctca tcgccgccgt cccgttgacg aactcggtct cgccaacggg cttcatctcg
     1801 accgggccgt cggcgcggta catcgagccg cactcgataa aaaccgtctg ggttatgttg
     1861 tggccactgc cagtgtcggt gagcagctgg tcgatcagaa aacggtgccc g
//
LOCUS       DATLJT010001236         9624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1012669,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001236 DATLJT010000000
VERSION     DATLJT010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012669"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="phnD"
                     /locus_tag="VM910_28450"
     CDS             complement(<1..495)
                     /gene="phnD"
                     /locus_tag="VM910_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI66478.1"
                     /translation="MMNRRFVIAGIASLAFTTSVFAEDWKAKYPEITFAVIPAENGSG
                     VTERFTPFVNYLSKELGIKVNLRVANDYAAVIEGQRAGNIHIGYYGPASFSRALLTGV
                     KTDAFVIDVNSDGSKGYYSVFYVLAKSPYQKLEDLKGKNLGLVDPNSTSGNNMPRFKM
                     NQMGI"
     gene            complement(562..1377)
                     /gene="phnC"
                     /locus_tag="VM910_28455"
     CDS             complement(562..1377)
                     /gene="phnC"
                     /locus_tag="VM910_28455"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HVI66479.1"
                     /translation="MLLVEGLTCRFGTKAAVDNASFSVAPGGFIGVIGRSGAGKSTLL
                     RTINRLTPATSGRILFDGTDVTALKAQELRRWRARSAMIFQQFNLIGRLDVLTNVLMG
                     RLSDIPSWRSLLQFWPEEDVAVAMSALEQFDMAQFAAQRADRLSGGQQQRVAIARALV
                     QEPDIILADEPIASLDPRNTKIVMDALLRINKHFGITVLCNLHSLDLARGYCDRLIGM
                     ASGRIVFDGAPSALTEHIARELYDLEASEVIGTVPSDVPDPSNVPALGKAAVA"
     gene            1564..2232
                     /locus_tag="VM910_28460"
     CDS             1564..2232
                     /locus_tag="VM910_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HVI66480.1"
                     /translation="MAGKTLSIEPTIDATAKLQGAKLGAYCEVGARTVLQDVEMGDYS
                     YVVNDAQITYTSIGKFCSIAAMTRISPGNHPMQRATQAHFTYRASEYFPGESDEAEFF
                     EWRKGHHVHIGHDVWIGHGAIILPGRSVGTGAVVAAGAIVTKDVAAYTIVGGSPAGPI
                     RRRFTEDIAERLVRLAWWDWHHQALRAALPDFRKLAIEVFLEKYEAATHSLHSNTPRR
                     SALT"
     gene            2229..3422
                     /locus_tag="VM910_28465"
     CDS             2229..3422
                     /locus_tag="VM910_28465"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HVI66481.1"
                     /translation="MTDIFIKGGRALLNGEILETSLSVADGKIRGIGSHQERGALELD
                     ARDLMVLPGIVDLHGDAFERQMMPRPGVDFPIDVALIDSDRQAIANGMTTVFHATTWS
                     WEPGLRSGDNAERLLAAIEALRPRLVADTRFHLRHETYNLDAEEKIGQWLAQDRIDLF
                     AFNDHMDITTALRNKPRKRATMIERTGLSDEAFEDLVERVLSRADEVPGSIARLAAIA
                     RGAGVRMLSHDDKSPEERRAFRAQGIEIAEFPVNEETAREAAAAGDAIVFGAPNVVRG
                     GSHTGWTKASDMIAKGLCSVLASDYYYPAPLIAAFRLAADRVLPLSQSWNLISATPAR
                     ACGLFDRGVLAEGHRADILLVDDAVPLRPRIVAVIVTGRLVHLTEAERLSASRASPRP
                     AVAAE"
     gene            3442..4143
                     /locus_tag="VM910_28470"
     CDS             3442..4143
                     /locus_tag="VM910_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HVI66482.1"
                     /translation="MTLFPRYAIYYAAAPGCALDHFGAELLGYDAWTGGDLPFPEDVL
                     RTVPDWQSLSEDPRKYGFHATLKAPLPLADGKTESELLAACAEFANMPRPIPVIRPVV
                     DSISGFIAVTPAERSSELEALAAECVSAFDSFRAPLTAEERTRRNQHNLTPGQRDYLE
                     RWGYPYVMEEFRFHMTLTGRLDTARREPVLAMLRRRFATIGLERLAIDRIALFRQHNA
                     VSKFRILMDFPLRAS"
     gene            4318..5637
                     /gene="dctA"
                     /locus_tag="VM910_28475"
     CDS             4318..5637
                     /gene="dctA"
                     /locus_tag="VM910_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HVI66483.1"
                     /translation="MATITTAETTARKPLYSSLFLQVVAALLLGIVLGMAVPDFAVSL
                     KIFSDAFLKLISMIVAPIVFCVVVHGIAGAGDLKKVGRVGVKALVYFEAMTTVALVLG
                     LLLAYLFGPGHGMNIDPSTLDAKALSNYAGNAQKLKGEGVGSFLMNIIPTTSFDALAR
                     NDVLQVLFFAILFGVALALVGGEKGERVSSMIDAVSIVLFRAMGLIVRVAPIGVLGAV
                     AYTVGKYGVGSLKQLVSLVVLFYVSVTIFVLGVLGSVMALAGLNIIKFLNYLREELTI
                     VLATASSDAVLPQIMRKLERMGVKDSVVGLVIPTGYSFNLDAFSIYLTLAVVFIAQAT
                     NTPLSLGDLLLVLGVSLVTSKGAHGVPGSAIVILAATLNAVPSIPAIGLVLVLSVDWF
                     IGMARALGNLIGNCVATVVVAAWEGDLDRAKATQVLDGAELVDVTAG"
     gene            complement(5638..6915)
                     /locus_tag="VM910_28480"
     CDS             complement(5638..6915)
                     /locus_tag="VM910_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HVI66484.1"
                     /translation="MSKTKTSATGLQSVPLGEEIAGRITELGAISESKAHLTRLFLTS
                     EHRAAADLILGWMRDAGMRAHIDAIGNVCGRYEGERPGLPCLMLGSHYDTVRDAGEWD
                     GPLGLITAISCVANLNRNGRRMPFAIEVVGFADEEGVRFASTLLGSRAVAGTFDESVL
                     SARDQAGIALRDALKDFGLDPGHIGVAARRRRELLAYLELHIEQGPVLEEQQLPVGVV
                     TAIAGATRLSVRLAGMAGHAGTVPMALRRDALTGAAECIGAIEEFCRTDDGGLVGTVG
                     YIHAAPGATNVIPGQVSFTMDIRAPTDQRRKRAVTDMVRRIEAIAKRRKLELQLNVTH
                     ENRTVPCAPWLKEQIAEAIAAEGFRVFELPSGAGHDGMAMIDVADVGMIFVRCRGGVS
                     HHPDEHVELADADAGARVLLRIIENFRPQPAQN"
     gene            7349..8458
                     /locus_tag="VM910_28485"
     CDS             7349..8458
                     /locus_tag="VM910_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI66485.1"
                     /translation="MDLGWISRRHLLQGSAALTLGSAFGVRTAAAADTTIGFIYVGSR
                     DDYGYNQAHAQGAAALKKLPGLKVVEEEKVPETDAVEKTMESMINLDGATLLFPTSFG
                     YYNPHMIKLANKYPKLRFEHCGGLWTEKDPKNAGSYFGYIDEAQYISGIVAGYSTKSG
                     KLGFVAAKPIPQVLRNINAFTLGAKLANPKATTQVIFTGDWSMPVKEAEATNSLIDQG
                     IDVLTCHVDGPKTMVENAARRGAMVCGYHVNQSPLAPKAYLTGAEWNWEALYPKFVKM
                     IAAGESIPNFYRGGLKEEIVKVSPYGEAVSAEARKHADDVKAKFMAGDYTIFKGPMSD
                     NKGKTVIASGVDRGQKDPELEKMDYLVEGVIGATS"
     gene            8455..9564
                     /locus_tag="VM910_28490"
     CDS             8455..9564
                     /locus_tag="VM910_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66486.1"
                     /translation="MTSDVADSAEAATIAPAADTGFLQRHGTTIEYMLIPGAALAGAL
                     VVFGIFVALFGKNPLDLYFYMYQGAFGTWFSWQNTLTRAAPLILTALCTALPAQLGMV
                     IIGGEGALLIGALAATSTALTVQTMPPLIVQFAMVCAGMLGGGLWIMLSGALRQYRGV
                     NETISSLLLVYIALAILNHLVEGVMRDPASLNKPSTREIGAANMIGTIPGTEVHWGLV
                     FGLIATIASYVLIYHTTFGFAARVAGGNVRAAKIVGLSVGKLILTICFLAGACAGLAG
                     MVEVAAVQGRTNANLAAGYGFTGILVAFLARQNPLAVVPVAILLGGISASGGLLQRRL
                     GLPDASVLVLQGIIFVFVLASDALYGRIGFLKGKS"
BASE COUNT         1799 a         2986 c         2973 g         1866 t
ORIGIN      
        1 gattcccatc tggttcatct tgaagcgcgg catgttgttg ccggaggtcg agttgggatc
       61 gaccaggccg aggttcttgc ccttcaaatc ctcgagtttc tgatagggac tcttggcgag
      121 cacgtagaac accgagtagt agcccttcga gccgtcggaa ttgacgtcga tcacgaacgc
      181 atcggtcttg acgccggtga gaagggcccg cgagaacgac gcaggtccgt aatagccgat
      241 gtgaatgttg ccggcgcgct ggccttcgat gacggcggca tagtcgttgg cgacacgcag
      301 gttcaccttg atgccaagtt ccttcgacag atagttgacg aagggcgtga agcgctcggt
      361 gacgccggag ccgttttcgg ccggaatgac cgcaaaggtg atctccggat atttggcctt
      421 ccagtcttcc gcaaagacgg acgtggtgaa ggccagcgag gcgatgccgg cgataacaaa
      481 tctgcggttc atcatggtgc accctctagg gttgggttgg catttttcat cctcgaacag
      541 ttccttcgag gatgcgggca gtcaggccac ggcagccttg ccgagagccg gaacgtttga
      601 agggtcgggc acgtccgagg gcaccgtacc gatgacctcg ctggcctcga ggtcatagag
      661 ctcacgcgca atgtgctcgg tgagcgcgga cggcgcgccg tcgaacacga tgcggccgga
      721 cgccatgccg atcaggcggt cgcaatagcc gcgcgccaga tcgagggaat gaagattgca
      781 gagcacggtg atgccgaagt gcttgttgat gcgaagcagc gcgtccatca cgatcttggt
      841 gttgcgggga tcgagcgagg cgatcggttc gtccgcaagg atgatgtcgg gctcctggac
      901 cagggcacgg gcgattgcaa cgcgctgctg ctggccgcct gaaagccgat cggcgcgctg
      961 ggccgcgaac tgcgccatgt cgaactgctc gagcgccgac atagccactg ccacgtcctc
     1021 ctccggccag aattgcagca gcgatcgcca ggacgggata tctgacagcc gtcccatcag
     1081 cacgttggtc agcacatcga gacgaccgat cagattgaat tgctggaaga tcatggccga
     1141 cctggcgcgc cacctgcgca gctcctgcgc tttcagagcc gtcacgtccg tgccgtcgaa
     1201 gagaatacgc cccgaggtcg ccggtgtcag acggttgatg gtcctgagca gcgtggactt
     1261 gccggccccg gaccggccga tcacgccgat aaagccgccc ggagcgacgg aaaatgaagc
     1321 attgtcgacc gcggcctttg tgccgaaacg gcacgtcaac ccctctacta acagcatcga
     1381 ttgctccagg cgttcgcggg aggcccatcg ctatcgttcg gattcaaaag ttttgtgaca
     1441 gggcactccg ctgtgaccat cgtccacact cgaagagagc gtcacgcaaa cgtcatgcat
     1501 ttgtcattaa gcccgtcgat gacgggcgcg cctgaagaca acatggcaag cttggaaggt
     1561 cgcatggccg gcaagacgct ctcgattgaa cccaccatcg atgccaccgc caagctgcag
     1621 ggagctaaac ttggcgccta ttgcgaggtc ggcgcccgca cggttctgca ggacgtcgag
     1681 atgggcgact actcctacgt cgtcaacgac gcccaaatca cttacacctc gatcggaaaa
     1741 ttttgttcga tcgccgcgat gacgcggatc agtcccggca accacccgat gcagcgcgcg
     1801 acccaggcgc acttcaccta ccgcgccagc gaatattttc cgggcgagag cgacgaggcc
     1861 gaattcttcg aatggcgcaa aggccaccac gtacacatcg gccatgacgt ctggatcggg
     1921 catggcgcga tcatcttgcc cggtcgcagc gtcggcaccg gcgcggtggt tgcggccgga
     1981 gcgatcgtta ccaaggacgt tgccgcctac accatcgtcg gcggcagtcc ggcggggccg
     2041 atcaggcgac gctttacgga agatatcgcc gaacgcctgg tgcgcctggc gtggtgggac
     2101 tggcaccacc aagcgcttcg ggctgcgctg cccgatttca ggaaacttgc gatcgaggtg
     2161 tttctcgaga aatatgaagc ggcaacccac tcactacact cgaacacccc gcgacgaagt
     2221 gccctcacgt gaccgatatt ttcattaaag gcggccgcgc gctgctgaac ggagaaattc
     2281 tggaaacctc gctgtcggtc gcggacggca aaatccgcgg catcggttcg caccaggaac
     2341 gcggcgcgct tgagctcgat gcgcgcgacc tcatggtgct gccgggtatc gtcgatctgc
     2401 acggcgatgc cttcgaacgg cagatgatgc cgcgcccggg tgtcgacttt ccgatcgatg
     2461 tcgcgctgat cgacagcgac cgccaggcga tcgccaacgg catgaccacg gtgttccatg
     2521 caacgacatg gtcgtgggag ccggggctgc gtagcggcga caatgccgag aggctgttgg
     2581 cggcgatcga ggccttgcga ccgcggcttg tcgccgacac gcgctttcat ctgcgccacg
     2641 agacctacaa tctcgatgcg gaagagaaga tcggccaatg gctggcccaa gaccgcatcg
     2701 atctattcgc attcaacgat cacatggaca tcaccacggc gctgcgaaac aagccccgga
     2761 agcgcgccac aatgatcgaa cgcacgggac tctcggacga ggcgttcgag gatttggtgg
     2821 agcgcgtgct gtcccgcgcc gacgaagtac ccggatcgat cgcgcgtctg gcagcgatcg
     2881 cacgcggggc cggtgtgcgg atgctctcgc atgacgacaa atcaccggag gagcgaaggg
     2941 cgtttcgtgc tcagggcatc gagatcgccg aattccccgt caacgaggag acagcgcggg
     3001 aggcggcggc ggcgggcgac gcgatcgtgt tcggcgcacc caatgttgtg cgcggaggca
     3061 gccatacagg atggaccaag gcgtccgaca tgattgcaaa gggtttatgc tcggtgcttg
     3121 cctcggacta ttattatcca gcgccgctga tcgccgcgtt ccgccttgcc gccgaccggg
     3181 tgctgccgct ttcacaaagc tggaacctga tctccgcgac tccggctcgc gcttgcggcc
     3241 tcttcgatcg tggagtgctc gccgagggac atcgcgccga tattcttctt gtcgacgacg
     3301 cggtgccgtt gcgaccgcgc attgtcgcgg ttatcgtcac cggccgtctg gtgcatctga
     3361 ccgaagccga gcgtcttagc gcttcgcgcg ccagcccgcg tcctgccgtt gctgcggagt
     3421 gaagcgtctc aatccacgcc gatgacactt ttcccacgct atgcgatcta ttatgccgcg
     3481 gctcccggct gcgccttgga ccacttcggt gccgaactcc tgggttatga cgcctggaca
     3541 ggtggggacc tgccgtttcc cgaagatgtg ctgaggacag tgcctgattg gcagtccctg
     3601 agcgaagatc cacgaaagta cggctttcac gccacgctga aggcgccgct gccgctcgcg
     3661 gacggcaaga ccgaatccga gcttcttgcg gcttgcgccg aattcgccaa catgccgcga
     3721 ccgattcccg tcatcagacc agtggtcgat tcgatcagcg gcttcatcgc cgtgacaccg
     3781 gcagaacggt caagcgagct cgaggcactt gccgccgaat gcgtcagcgc gttcgatagc
     3841 tttcgcgcac cgcttacagc cgaagagcgc acgcgacgca atcagcacaa cttgacgcca
     3901 ggccagcgcg actatctgga gcgctggggc tatccttatg tcatggaaga gtttcgcttc
     3961 cacatgacac tgaccggccg gctcgacacg gctcgccgcg agcccgtcct cgcaatgctg
     4021 cggcgccgct ttgcgacaat cggactcgaa cggctcgcga tcgatcgcat tgccttgttt
     4081 cgccagcaca acgccgtttc gaagtttcgt atcctcatgg atttcccgct gcgtgcgtct
     4141 tgaggcacgt atggtcctga ccgcagtcac aattgcagca ggggtcacgc tcggcccacc
     4201 cttgccgggc tcgggacgac cccggcaaat tctgtgattt cggaggtgct taaccctcgc
     4261 tgagaaactg gcacggttgt tgctgagccg gctggagatc aatcggggga acgcaatatg
     4321 gcaaccatta ccaccgccga aaccactgca cggaagccgc tctattcctc actgttcttg
     4381 caagttgtgg ccgcgctgct gctcggaatt gtactcggca tggcggttcc cgactttgcc
     4441 gtcagcctga agatcttcag cgacgctttc ctgaagctga tctcgatgat cgtcgcgccg
     4501 atcgtgttct gcgtcgtggt gcacggcatt gccggcgccg gcgacctcaa gaaggtcggc
     4561 cgcgtcggcg tcaaggcact ggtctatttc gaggcgatga cgacggtggc gctcgtgctt
     4621 gggcttttgc tcgcttattt gttcggcccg ggccacggca tgaacatcga tccgtcgacg
     4681 ctcgatgcca aggcgctgtc caattacgcc ggcaacgcgc agaagctgaa gggcgaaggc
     4741 gtcggctcct tcctgatgaa catcatcccg accacgtcct ttgacgcgct ggcgcgtaac
     4801 gacgtgctgc aggtcctgtt cttcgcgatc ctgttcggcg tcgcgcttgc gctggtcggc
     4861 ggcgaaaaag gcgagcgcgt cagttcaatg atcgacgcgg tgtcgatcgt gctgttccgc
     4921 gcgatgggct tgatcgtacg cgtcgcaccg atcggcgtgc tcggcgcagt cgcctatacg
     4981 gtcggaaaat atggcgtcgg ttcgctgaag caactcgtgt cgctggtcgt gctgttctat
     5041 gtctcggtca cgatctttgt gctcggcgtg ctcggcagcg tcatggcgct tgccgggctg
     5101 aatatcatca agttcctcaa ctatctccgc gaggagttga ccatcgtttt ggcgaccgcg
     5161 tcgtccgatg cggtgctgcc gcagatcatg cgcaagctcg agcgcatggg ggtgaaggat
     5221 tccgtcgtcg gtctcgtcat tccgaccggt tattccttca atctcgacgc cttctcgatc
     5281 tatttgacgc tcgccgtggt gttcatcgcg caggcgacga acacgccgct gtcgctcggc
     5341 gatctcctgc tggtgcttgg cgtgtcgctg gtcacgtcga agggcgccca tggcgtcccc
     5401 ggctcggcga tcgtgatcct ggccgcgacc ttgaacgcgg tgccgtcgat cccggcaatc
     5461 ggtctcgtgc tggtgctgtc ggtcgactgg ttcatcggca tggcgcgcgc actcggcaat
     5521 ttgatcggca attgcgtcgc aaccgtcgtc gtcgcggcct gggagggcga tctcgatcgc
     5581 gccaaggcaa cgcaggtgct cgacggcgcc gaactcgtgg acgtcacagc aggctgatca
     5641 attttgggca ggctgcggcc gaaagttctc gataatacgc agcaacacgc gcgcgccggc
     5701 gtcggcgtcg gcaagctcaa catgctcgtc cggatgatgg ctgacgccgc cgcggcagcg
     5761 cacgaagatc atgccgacat cggccacatc gatcatcgcc atgccgtcgt gccctgcccc
     5821 gctcggcagc tcgaacacgc gaaagccctc ggctgcaatg gcctccgcga tctgttcctt
     5881 cagccagggc gcgcagggaa cggtgcggtt ttcgtgggtg acgttgagct gcaactcaag
     5941 cttacgccgc ttcgcgatcg cctcgatccg gcggaccatg tcggtgaccg cgcgcttgcg
     6001 gcgctgatcg gtcggcgcgc gaatatccat cgtgaacgac acctggccgg gaatgacgtt
     6061 ggtcgcgccg ggcgctgcat ggatatagcc gacggtgccg accagccccc cgtcatccgt
     6121 gcggcagaac tcctcgatcg cgccaatgca ttcggccgcg cccgtcagcg cgtcccggcg
     6181 gagcgccatc ggtaccgttc cggcgtgacc cgccatcccg gcgagtcgca cggacagacg
     6241 cgttgcacct gcgatggccg tcacgacgcc gactggaagc tgctgctcct ccagcaccgg
     6301 cccctgctcg atgtgcagtt cgagataggc gagcaattca cgccgccttc ttgcggcaac
     6361 gccgatatgg ccggggtcaa gcccgaaatc cttcagcgca tcgcgcaagg cgatcccggc
     6421 ttgatcgcgc gcgctcagca cgctctcgtc aaaagtgccg gcgacggcgc ggctgccgag
     6481 caaggttgag gcaaagcgaa ccccctcttc gtcggcaaaa ccaaccactt cgatcgcaaa
     6541 cggcattcgc cgtccgttcc tgttcaggtt ggcgacgcag gaaatcgccg tgatgaggcc
     6601 gagcggaccg tcccattcac cggcgtcgcg cacggtgtcg tagtgcgagc ccagcatcag
     6661 gcatggcagg ccggggcgtt cgccctcgta gcggccgcag acgtttccga tagcgtcaat
     6721 atgagcgcgc atgccggcgt cacgcatcca gccaaggatc agatcggccg ctgcgcgatg
     6781 ttcagaggtg agaaagaggc gtgtgagatg cgctttggac tcggaaattg cgccgagctc
     6841 cgtgatccga ccggcaatct cctcgccgag cgggacgctt tgaagcccgg ttgcgctcgt
     6901 tttcgttttg ctcatgcgtt ccaacgggct tcaagacggt tgatacaaat tcgatgccgg
     6961 cgaatgcgat cctgttcaga agtccgcatt gcgatctcgc ggcggtgcgg actactgaat
     7021 cgccacaccg ggcgactggt aacagcctca gccagcttga ccagtggcaa ttgatcgacc
     7081 cgcagcaggc gtccttcgag ctttcgaatg atgcttaact ctcttgcata caatcccaag
     7141 atgccggcta aaatttaggc ttgccagctt tggctcaaga ttttgtcaaa gaccgaaaat
     7201 ccttataatt taaatatttt caatagttta aattatgatg ctcagcaaaa aggctgtggc
     7261 atgaagcttg cgacaaagcc tcgcgggcat tgccgaccgg gcgaggccgc gaagtgaggg
     7321 gcggtgcgtc cgccaacggg agcaaccaat ggatcttggc tggatttcac gacggcatct
     7381 gttgcaaggg agcgcggcgt tgacactcgg ctccgccttc ggcgtccgaa ccgcggccgc
     7441 cgccgacaca acgatcggct ttatctatgt cggctcgcgc gacgactacg gctacaacca
     7501 ggcgcatgcg caaggcgctg ccgcactgaa gaaattgccc ggcctcaaag tggtcgagga
     7561 agagaaggtc ccggagaccg acgccgtcga aaaaacaatg gagtcgatga tcaatctcga
     7621 cggggccacg ctgctgttcc caacctcgtt cggttactac aacccgcata tgatcaagct
     7681 ggccaacaaa tatccgaagc tgcgcttcga gcattgcgga ggcctttgga cggaaaagga
     7741 tccaaagaac gccggcagtt atttcggcta catcgacgag gcgcaatata tctccggcat
     7801 cgtcgcaggc tattccacca agagcggcaa gctcggcttc gtcgcggcaa agccgatccc
     7861 gcaggtcctg cgcaacatca atgcctttac tctgggcgcc aagctcgcca atcccaaggc
     7921 gacaacgcag gtgatcttca ccggcgactg gtcgatgccg gtcaaggagg ccgaggcaac
     7981 caacagcctg atcgaccagg gcatcgacgt cctgacctgc catgtcgacg gaccgaagac
     8041 catggtcgag aacgcggcgc gccgcggcgc catggtgtgc ggctatcacg tcaaccagtc
     8101 gccgctcgca cccaaggctt acctcaccgg cgcggagtgg aactgggagg cgctctatcc
     8161 gaaattcgta aagatgatcg cggccggcga aagcatcccg aacttctatc gcggcgggct
     8221 gaaggaagag atcgtcaagg tttcgcccta cggcgaggct gtttccgcgg aggcacgcaa
     8281 gcacgccgac gatgtcaaag ccaaattcat ggcgggcgat tatacgattt tcaagggccc
     8341 gatgagcgac aacaagggca agacggtgat tgcgtcgggt gtcgaccgcg gccagaagga
     8401 tccggagctc gaaaagatgg attacctggt cgagggcgtg atcggagcaa cttcgtgacc
     8461 tccgacgttg cggattcggc agaggcggcc acgatcgctc cggccgccga tacgggattc
     8521 ctccaacgtc acggcacaac catcgaatac atgctgattc cgggcgcggc gctcgccggc
     8581 gcgctcgtgg ttttcggcat cttcgtcgcg ctgttcggca agaacccgct cgacctctat
     8641 ttttacatgt accagggcgc gttcggcacc tggttctcct ggcagaacac cttgacccgc
     8701 gcagcgccgc tgatcctgac cgcactttgc acggcgctgc ccgcccaact cggcatggtg
     8761 atcatcggcg gcgaaggcgc gctcctgatc ggcgctttgg cggcaacctc gaccgcgctg
     8821 acggtgcaaa cgatgccgcc gctcatcgtc cagttcgcaa tggtctgcgc cggtatgctc
     8881 ggcggcggcc tctggatcat gctatcgggc gcactgcgtc aataccgcgg cgtcaacgaa
     8941 acgatttcga gcctgttgct cgtctatata gcgctcgcga ttctcaacca tctggtcgag
     9001 ggcgtgatgc gcgaccccgc aagcctcaac aagccctcga cgcgcgagat cggcgcagcc
     9061 aacatgatcg gaaccattcc cggcaccgag gtgcattggg ggttggtatt cggtctgatt
     9121 gcgacgatcg cctcctacgt cctgatctat cacaccacct tcggctttgc cgcgcgcgtc
     9181 gccggcggca atgttcgcgc cgccaagatc gtgggcctct ccgtcggcaa attgatcctg
     9241 accatctgct ttcttgcggg cgcctgcgca ggattggcgg gcatggtcga ggtcgccgcg
     9301 gttcagggcc gcaccaacgc caatctcgcc gccgggtacg gatttacggg catactggtc
     9361 gccttccttg cgcggcaaaa tccgctcgcg gtcgttccgg tcgcgatcct gctcggcggc
     9421 atcagcgcga gcggcgggct gctgcagcgc aggcttggcc tgcccgatgc atcggtcctg
     9481 gtcctacagg ggatcatctt cgttttcgtg ctcgcgagcg atgcgcttta cggacggatc
     9541 ggttttctga aagggaagtc ctgacatggc agccggttcg atcggcctct ggacggtacc
     9601 gcttgccgtg ttcggcggtg ccat
//
LOCUS       DATLJT010001237         3610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2162603,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001237 DATLJT010000000
VERSION     DATLJT010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162603"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VM910_28495"
     CDS             complement(<1..623)
                     /locus_tag="VM910_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI66487.1"
                     /translation="MKIHVGFDLTYEIAHPTPMIFMLNVHPSRAADLITPDRLRIAPS
                     RSITPYIDGFGNKCVRLLAPPGELRVASDTIVSDSGRADRVDVGAEEHAVADLPHDTL
                     VYLLASRYCEADSMMDKAWEIFGQAPRGWRRVQAICDFVHSHITFGYEHADATRGAVR
                     VFVEQRGVCRDFAHLAVTFCRCLNIPARYCTGYLGDIGVPPDPAPMD"
     gene            795..1700
                     /locus_tag="VM910_28500"
     CDS             795..1700
                     /locus_tag="VM910_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI66488.1"
                     /translation="MTILSVRHVTTYLYRKPVAFGEHRLMFRPRDSYDQRLIDSSLLI
                     TPEPVSIHWIHDPFGNCVALARFKRRASELRFESRIRVDHYPWNVPDFQTDPQAKTYP
                     FTYSPDEIPDLAPSIARGYPDPDGEIDRWVRKFLRPGRPTPTGRLLMTLTHGIKEGFT
                     YTRRAEHGTQEPVVTVRIGRGSCRDFALLMIEAVRSLGLAARFVSGYLHVPSRDGPEY
                     AGGGSTHAWCQVYLPGAGWVEFDPTNGIIGNRDLIRVAVARDPSQAVPLSGTYVGNSE
                     DELGMKIEVSVKCESDPRNRQSKRS"
     gene            1697..2026
                     /locus_tag="VM910_28505"
     CDS             1697..2026
                     /locus_tag="VM910_28505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66489.1"
                     /translation="MNEIVHLPPMSARRETLVPARPKQEKIGATLAIPVHPHLQTVLD
                     ATPSAHLTFLVTATGKPYGGNALSEQFRNWCDAPRLPKRCKPHGPRKAADQARLARNA
                     LAKAVIR"
     gene            2023..3006
                     /locus_tag="VM910_28510"
     CDS             2023..3006
                     /locus_tag="VM910_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66490.1"
                     /translation="MTRRREFISLLGGAAVAWPLAARAQPTAMPVIGVLGPGSAESNA
                     FRVTPFRQGLNESGYVLGQNLMVEFRWAESQYDRLPALAADLAQRQVAVIVTIANVAS
                     LAAKAATTTIPIVFTAGADPVQLGLVASLNRPGGNATGVTFFTTSLEPKKLELLRELV
                     PAATVIALLINSTGPNAETTLRSVRAAADALGLQIQVVNASSAREFDAAIATAVGQGA
                     RALFVGADPLFTDERDKLVPVIARHAIPAIYSFREATMAGGLMSYGISFAEVYRQAAM
                     YTARILKGTKPLDLPVIRTTKFELVINLKTAKALGLTVPQSLQVAADEVIE"
     gene            complement(3190..>3610)
                     /locus_tag="VM910_28515"
     CDS             complement(3190..>3610)
                     /locus_tag="VM910_28515"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66491.1"
                     /translation="PGATRVAVLVNPANPATETTLRDVEPAARAMGLQLQFLKAGTSR
                     EIDAAFATFVRERPDALFVGGDSFFVSRRFQLVNLASRHAVPATFSNRDFAEVGGLIS
                     YGANPTDAYRQVGVYAGRILKGAKLRTCRSCSRASSI"
BASE COUNT          766 a         1074 c         1038 g          732 t
ORIGIN      
        1 aagtccatcg gtgctggatc tggaggtaca ccgatatccc ccaaataacc cgtgcaatat
       61 ctcgctggaa tattcaggca ccggcaaaac gtgacggcaa ggtgagcgaa gtcgcgacac
      121 acgccccgtt gctcgacaaa aactctcacg gcgcctcttg ttgcatcggc atgttcatag
      181 ccaaatgtga tatgcgaatg aacgaaatcg cagatcgctt gcacccttcg ccagccgcgg
      241 ggagcctgcc caaatatctc ccacgccttg tccatcatcg aatccgcctc gcaatatcgg
      301 ctcgcgagga ggtacaccaa agtgtcatga ggtaagtcgg ccaccgcatg ctcctcagcc
      361 cccacgtcga cacggtcagc cctaccgcta tcagagacaa tggtatcgct tgcgacccgc
      421 aattcgccag gaggcgctaa aagccgtaca cacttgtttc cgaacccatc gatataaggc
      481 gtaattgaac gcgacggggc gatgcgcagc ctgtcgggag tgattaagtc ggctgcgcgc
      541 gacggatgca cattaagcat gaatatcatt ggcgtcggat gtgcgatctc gtaagttagg
      601 tcaaagccca cgtggatttt cactatcgct cctcgtgttc agttagcccc cacctttatc
      661 cccgttcaga tgaccgggcc agcgcagcga tgcacaaatt ttcagcagga cttgtaagca
      721 actcgtatca accccgccct gaaattgcga tttagtggac aacatgcttt cgtgattcgc
      781 gatcagcttg cgtcatgaca attctgtcgg tccgccacgt taccacttac ctctatcgaa
      841 agccggtcgc cttcggcgag caccgcttga tgttccggcc acgcgacagc tatgaccagc
      901 ggctgatcga ctctagtctt ctcatcactc ctgagccggt cagcatccac tggatccatg
      961 acccgttcgg caactgtgtc gccttggcgc ggttcaagcg gcgagcgtcc gaattgcggt
     1021 ttgaaagtag gattcgtgtg gatcattacc cctggaacgt gccggatttt caaaccgacc
     1081 cgcaagctaa gacctatccg ttcacctaca gccccgacga aattccagat ttggcgcctt
     1141 cgattgctcg tggctatccc gatccggacg gtgagataga ccggtgggtg cgcaagttct
     1201 tgcggccagg acgcccgaca ccgacaggaa ggctgttgat gactcttaca catggcataa
     1261 aggaggggtt cacctatacc agacgcgccg aacacggcac gcaggagcca gttgtgacag
     1321 tgaggattgg acgtggcagt tgccgcgact ttgcgttatt gatgatagaa gcggtccgct
     1381 cgctcggtct cgcggcccgt ttcgtttcgg gctatctgca tgttccttcg cgtgatggtc
     1441 ccgaatatgc cggcggtggc tcgacccacg cgtggtgcca agtctatctg ccgggcgccg
     1501 gctgggtcga atttgatcca acaaacggta tcattggaaa tcgtgacctc atccgcgtcg
     1561 ctgtcgcgcg tgaccccagc caagcagttc cgctctccgg tacctacgtt ggaaattcgg
     1621 aggacgagtt aggtatgaag atcgaggtca gcgtaaaatg tgagtctgat ccccgtaatc
     1681 gtcaatcgaa aagatcgtga acgagatcgt ccacctccct ccgatgtccg cacggcgcga
     1741 gaccctcgtt ccggcacggc cgaagcagga aaagatcggc gcgaccttgg cgatcccggt
     1801 gcatccgcac ttgcagacag tgctcgacgc cacgccgagc gcgcacctga cgtttctcgt
     1861 caccgcgacc ggcaagccgt acggcggcaa cgcgctcagc gagcaatttc gaaattggtg
     1921 cgacgcgccc aggctgccga agcgctgcaa accgcacggg ccgcgcaagg cggcggatca
     1981 ggcgcggctc gctcgcaatg cgttggcgaa ggcggtcatc cgatgacccg ccgccgcgag
     2041 ttcatatcgc tgctcggcgg cgcggcggtc gcatggccgc tggcggcgcg cgcgcagccg
     2101 acggcgatgc cggtgatcgg cgtgctcggc cccggctcgg ccgagtccaa cgcctttcgt
     2161 gtgactccat tccggcaggg actgaatgaa agcggctatg tcctgggcca aaacttgatg
     2221 gttgaattcc gatgggctga gagccaatac gatcgactac cagcgctcgc ggctgatctc
     2281 gctcagcgtc aggtggccgt cattgttact atagccaatg ttgcgtcgct ggcggcgaag
     2341 gcggctacaa cgacaattcc gattgtcttc acggctggtg ccgatccggt tcagcttggt
     2401 ctggttgcca gcctcaatcg gccgggcggc aatgccacgg gtgtaacctt cttcaccacc
     2461 tcgctcgaac cgaaaaagct ggaactacta cgagaattag tccccgcggc caccgtgatc
     2521 gcgctcctta tcaattcaac tggtccaaac gcagaaacaa ctttgagaag cgtgcgggcc
     2581 gccgcagatg cgctcgggct acaaatccaa gttgtcaatg ccagcagcgc gcgagagttc
     2641 gatgcagcaa ttgcaaccgc agttggacaa ggggcgcgtg cattgtttgt gggcgccgat
     2701 cctctgttca ctgatgagcg cgataaactg gtgccggtta tagcccggca cgcaattccg
     2761 gcaatttatt cgtttcgcga ggccacgatg gctggcggcc tgatgagcta cggtatcagc
     2821 ttcgccgagg tgtaccgcca ggccgcaatg tacactgcaa ggattcttaa aggtacgaag
     2881 cccctcgacc tgcctgtcat tcggacaacg aagtttgaac tggtgatcaa tctcaaaacc
     2941 gccaaggcgc ttggcctcac cgtgccgcaa agcttacaag tagccgccga cgaggtgatc
     3001 gagtgaggcg tgaacgaagg atgactcgac agtgtaaaat ttccccgcgt tttacaattt
     3061 gggtgccaga agtgccgatt ccacgggctc gaaaatgcga cgaataactc gcggcggttc
     3121 attcgatcac ctcgtcggcg aggatttgca ttgagttcgg aaccgcgatc ccaagggtcg
     3181 tcgcggtttt catattgaac ttgctcgact gcacgaccgg caggtccgca gcttcgcgcc
     3241 cttgaggatg cggccggcat agacgccgac ctgacggtac gcatccgtcg ggttggctcc
     3301 atagctgata agcccgccaa cttcagcaaa gtcgcggttt gaaaatgtcg cggggaccgc
     3361 gtggcgcgac gccagattga ccaattggaa acgtcggcta acgaaaaagc tatcgccgcc
     3421 gacgaaaagc gcgtcgggcc gctcgcgcac aaaggttgca aaggccgcgt cgatctcccg
     3481 actggtgccg gccttgagga actggagttg cagacccata gcgcgagcag ccggttccac
     3541 gtctctcaac gtagtctcgg tagcgggatt agccggattg acgagcacgg ccacacgagt
     3601 ggcgccgggc
//
LOCUS       DATLJT010001238         1758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_93811,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001238 DATLJT010000000
VERSION     DATLJT010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93811"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..1529
                     /locus_tag="VM910_28520"
     CDS             537..1529
                     /locus_tag="VM910_28520"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66492.1"
                     /translation="MFAIKRRKFLTLFGGAVLAWPLAVRAQPMAKVWHVGMLETTAAT
                     LNATNLDAFKQALRQLGYIEGQNLIVEYRSGDGHIERFPQLAAELVRLNVDIIITRGT
                     PAALAAKKATATIPIVMAAIGEPVETGMVASLARPGGNVTGLSAFVTELTAKRIEIMR
                     EVIPQLSRMALIDNMANRSVPAQWDETKRAAFALGIQPQLYDVRKAEDIERAFSSAIA
                     QRVNALSVGNDSVVIANRIQVVQLAAKHRLPAIYATRDFVDAGGLLSYAAHYPDLYRR
                     AAAYVDKIFKGAKPADLPVEQPTKFEIVVNLKAASALGLTVPSTLLARADEVIE"
BASE COUNT          355 a          555 c          552 g          296 t
ORIGIN      
        1 gggcccagag ccacggaccg cggccccata gcgcgtcgaa gacgcgcgta aacgcgctga
       61 tggtttcccg ctttcgcggg aacgaacgga gtgagttgct accgctggga ttcatccccg
      121 cgaaagcggg gacgggaatg aacggagtac gttgctattg ctggcgttcg ccctcgcgaa
      181 accggcagcg ggagcggatg aggcgtgtac gcataaacgc agcattggcc gaatgtccgc
      241 gcttggggac taaccggaca tgcggggacg gcgggaatga cgcgaatgac ccaaagcgga
      301 catcggccgg atcgaaatct cgcagtgcac caacctcgtg ccgcaccgag tgtgctatcc
      361 tttactcgga agcacggacg gcgccgatag catcggcttc gattcagaac aattcaggtc
      421 tgccccaagg acttgccaac ccttctgagg caggctgaac gtgcggcaaa ccggaccgca
      481 ggagccggcg cctgggcctc taagacgagg tcagataaca ggggaggaac tcccccatgt
      541 tcgccataaa gcgacgcaag ttcctcacgc tgttcggcgg cgcagtcctg gcgtggccgc
      601 tcgcggtgcg cgcacagccg atggccaaag tctggcacgt cggcatgctg gaaacgacgg
      661 ccgccaccct caacgccacc aacctcgatg ccttcaagca ggcgctgcgc cagctcggct
      721 acatcgaggg acagaacctg atcgtcgaat atcgctccgg cgacggccac atagagcggt
      781 tcccgcagct ggcggccgag ctggtccgcc tcaacgtcga catcatcatc acgcgcggca
      841 cgccggcggc gctcgccgcc aagaaggcca ccgcgacgat acctatcgtc atggcggcga
      901 tcggtgagcc ggtcgagacc ggaatggtcg ccagcctggc acgaccgggt ggtaacgtca
      961 ccggcctgag cgccttcgtc accgagctga ccgccaagcg cattgagatc atgcgcgagg
     1021 tcattccaca gctttcgcga atggcgttga tcgacaacat ggcgaatcgc agcgttccgg
     1081 cgcagtggga cgagacgaaa cgagcagcgt tcgcgctcgg catccagccg cagctgtacg
     1141 atgtgcggaa agcggaggac atcgagcgcg cattcagctc ggcgattgct cagcgggtca
     1201 acgcgctgtc ggtcggtaac gacagcgtgg tgatcgccaa ccgcattcag gtagtgcagc
     1261 tcgcggccaa gcatcggctg ccggcgatct acgccacgcg cgacttcgtc gacgccggcg
     1321 gcttgctgtc ctacgcggcg cactatcccg acctctatcg ccgagccgcg gcctacgtgg
     1381 acaagatttt caagggcgcc aagccggccg atctaccggt cgagcagccg actaagttcg
     1441 agatcgttgt caatctcaag gcggccagcg cgctcggcct cacagttccg tcgacgttgc
     1501 tcgctcgcgc cgacgaggtg attgaatagc aatgcttttt gttgcggtgc atatgtcggc
     1561 tcgtggcaca tcgcgtcatt tcgctgccgc gcagcaattc ggtcgctttc ggagcgaagc
     1621 ggacatagac ccgcagcgga gggatcgaaa atcccacact acggcggtct gctggaaatg
     1681 aggtgcacat cgaggttctc attgcttccc agcaagcagc ctcggtgcac acctcaaggc
     1741 gaaatctctc cacttggt
//
LOCUS       DATLJT010001239         1980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_94083,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001239 DATLJT010000000
VERSION     DATLJT010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94083"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="VM910_28525"
     CDS             complement(<1..1965)
                     /locus_tag="VM910_28525"
                     /inference="COORDINATES: protein motif:HMM:NF009588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HVI66493.1"
                     /translation="MLFTARSLGRSKEMTLMQVGLDDKYRFEAKRIYLSGVQALVRLP
                     MLQRERDRLAGLNTAGFISGYRGSPLGMYDHALWRAKSFLQGEDIAFVPGLNEHLAAT
                     AVWGSQQVGLFPGAKVDGVFGIWYGKGPGVDRSMDVLKHANSAGSSQHGGVLALAGDD
                     HGCQSSTLAHQSEQVFAAALMPIINPATLQEYLDLGLYGFALSRYSGCWIGFKAIGET
                     VESSASIYSDVDRVQVMLPDDFELPPGGLNIRWPDPPLEQEKRLFGPKMEAAKAFARA
                     NRFDRIVLDGKPARLGIITTGKAYLDLRQALADLGISDRDAEALGLRIYKVALTWPLE
                     EVGAKRFAEGLQDVLVVEEKRGFIEDQLMRILYNVDAWKRPAVVGKRDEAGSPLLPSE
                     GELNPTIVASALVARLRRLGHHSPVLEQRLARLEAFDRPAPGHAPISLQRTPYFCSGC
                     PHNTSTKIPEGSRATAGIGCHGMALFMPARRTTTISQMGGEGVNWIGQAPFTNEPHVF
                     QNLGDGTYTHSGLLALRAAAASGVSITYKILYNDAVAMTGGQPAEGSFTVAQIAHQVW
                     AEGTKRLAIVSDEPDKYPRNYFPPGATVHHRREMDDLQRELRETKGLTVLIYDQTCAA
                     EKRRRRKRGLYPDPPKRVFINDLVCEGCGDC"
BASE COUNT          351 a          614 c          659 g          356 t
ORIGIN      
        1 acaatcgccg cagccttcgc agacgagatc gttgatgaag acgcgcttgg gcggatcggg
       61 atagagcccg cgcttgcggc ggcggcgctt ctcggcggcg caggtctgat cgtagatcag
      121 gaccgtgagc cccttggtct cacgcaattc gcgctgcaaa tcatccatct cgcggcggtg
      181 atgaacggtt gcgccgggcg gaaagtagtt gcgcggatat ttgtcgggtt cgtcggagac
      241 gatggcaagg cgcttggtcc cctcggccca gacctggtgg gcgatctgcg cgacggtgaa
      301 gctgccttcg gcgggctggc cgccggtcat ggcgaccgca tcgttataga gaatcttgta
      361 ggtgatgctc acgcccgacg cggctgcggc gcgcaaggcc aacaggccgg aatgggtgta
      421 ggtgccatcg ccgagattct ggaagacgtg cggctcattg gtgaagggcg cttgtccgat
      481 ccagttcacg ccctcgccac ccatctggga gattgttgtg gtgcgccgtg cgggcataaa
      541 gagtgccatg ccgtggcagc cgataccggc cgtggcgcgg ctgccctccg ggattttggt
      601 cgaggtgttg tgcgggcagc cggagcagaa ataaggcgtg cgctgcagag aaatcggcgc
      661 gtggcctggg gcgggccgat cgaaggcctc caaccgcgca agcctttgct ccaagaccgg
      721 gctgtgatgc ccgaggcgcc gcagacgcgc gaccaatgcg gaggcaacga tggtcgggtt
      781 gagctcgcct tcgctcggca gcagcgggct gcctgcctcg tcgcgcttgc cgaccacggc
      841 cggccgtttc caggcatcga cattgtagag aatgcgcatc aactggtctt cgatgaagcc
      901 gcgcttctcc tcgaccacaa gcacatcctg caagccttcg gcgaagcgct tcgctccgac
      961 ttcctcgagc ggccaggtca gcgcgacctt atagatgcgc aagcccagcg cctcggcgtc
     1021 gcgatcgctg atgcccaggt ctgcgagcgc ctgccgcaaa tcgagatagg ccttgccggt
     1081 cgtgatgatg ccaagccgcg ccggcttgcc gtcgagcacg atgcggtcga acctgttggc
     1141 gcgcgcgaag gccttggccg cttccatctt cggcccgaac aggcgcttct cctgctcgag
     1201 tggcggatcg ggccagcgga tattgaggcc gcccggcggc agttcgaaat catcaggcaa
     1261 cattacctgt acgcggtcga cgtcgctgta gatcgaggcc gagctctcca ccgtctcgcc
     1321 gatcgccttg aagccgatcc agcagcccga atagcgcgac aacgcaaagc catagagtcc
     1381 gaggtcgaga tactcctgca acgttgcggg attgatgatt ggcatcagcg ccgccgcgaa
     1441 cacctgctcg ctttgatggg cgagggtgga cgactggcag ccgtgatcgt cgccggcgag
     1501 tgccagcacg ccgccatgtt gggaagagcc tgccgaattg gcgtgcttca ggacgtccat
     1561 cgagcggtcg acgccggggc ccttgccgta ccagatcccg aacacgccat cgaccttcgc
     1621 gccggggaac aggccgacct gttgactgcc ccatacggcg gtcgcggcga gatgctcgtt
     1681 gaggccggga acgaaggcga tgtcctcgcc ttgcaggaac gacttcgcgc gccaaagcgc
     1741 atggtcgtac ataccaagcg gcgagccccg gtaacccgag atgaaacctg cggtgttgag
     1801 gccggccagt cgatcgcgtt cgcgctgcag catcggcaac cgcaccagcg cctggacacc
     1861 ggacagatag attcgcttgg cctcgaaccg gtatttgtcg tccagcccca cctgcatcag
     1921 cgtcatttct ttgctccggc cgagggagcg ggcagtgaaa aggatgtgtc ggtaatgtca
//
LOCUS       DATLJT010001240         1901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1474340,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001240 DATLJT010000000
VERSION     DATLJT010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474340"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VM910_28530"
     CDS             <1..1218
                     /locus_tag="VM910_28530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66494.1"
                     /translation="EAIAHLERGLGLLRSLPESQARNSRELELQLALGLCVHTAKGAV
                     AAKPPYMRAHELGERGGTPQQRFEALYGVWQSTNLSGGITAASPLSERLLSMTEREGD
                     DGLRLQAHHSGWATSYYAGDQAKAREHADAGRLLYDPTKHASHRFVYGGHDPGVCAGN
                     IGAQVEWLLGYPEKALASIADALALAERIAHPFTLSLVFINSSVIYLNRHEPERALRQ
                     LEAAEVLAAEQRLYLIYEPGMLRGVALRGQGAVDEAIARIREGITKWTRLGRTVYLPY
                     CLAFLAEGLARHGDRAAALAALREGLETARATGEHMWDAELHRLTGSVLLAENKLDEG
                     QASLQQAIRIAQAQRAKSLELRAARDLARLWGEQGRRTDARDLLTPVYGWFTEGFDTA
                     DLKEAKALLDELT"
     gene            complement(1395..>1901)
                     /locus_tag="VM910_28535"
     CDS             complement(1395..>1901)
                     /locus_tag="VM910_28535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI66495.1"
                     /translation="GIVYNPEPGNNSSAFRKSIDEVANKVGIVSVEAPSGDSSDIDRL
                     IRSLKDEPNSGLVFLPDAFAGARHVQITALVAQCRLPAIYPARLFCEAGGLMSYGIKA
                     EYGGKADTVTAGAASYVDLILRGANPAELPVQLPTEFEFVVNEKTARQLGLQLPPLLV
                     ARADDVIE"
BASE COUNT          384 a          572 c          584 g          361 t
ORIGIN      
        1 gaagcgattg cccatctcga gcggggtctc ggcctgctgc gttcattacc ggagagccag
       61 gcccggaaca gccgcgaact cgagctgcaa ctggcattgg gactctgtgt gcacaccgcc
      121 aaaggcgccg tcgcagcgaa gccgccctat atgcgggcgc acgagcttgg cgagagaggc
      181 ggcacgccgc agcaacggtt tgaggcgctc tacggggtgt ggcagtcaac taacctgtcc
      241 ggtggaataa ccgccgccag cccgctctcc gagagactgc tgagcatgac cgaacgggag
      301 ggagatgatg gattgcggct ccaggcgcat catagcggct gggcgacatc gtattatgcc
      361 ggggatcagg caaaagctcg cgagcacgcc gacgccggcc gccttctcta cgatccgacg
      421 aagcatgcgt cccatcgttt cgtctatggc ggacatgatc ctggtgtctg cgccggaaac
      481 attggcgctc aggtcgaatg gttgctggga tatcccgaga aggcgcttgc gagcatcgct
      541 gatgctttgg cgctggccga gcggatcgcc catccgttca cgctgagtct tgttttcatt
      601 aattcgtcgg tgatttacct caatcgccac gaaccagagc gagccctgcg ccagctcgaa
      661 gccgccgagg tgctggctgc ggagcaacgg ctctatttga tctacgaacc cggcatgctg
      721 cgcggcgtgg cattgcgcgg acaaggtgcg gtcgacgaag caatcgcccg cattcgcgag
      781 ggcattacga aatggacccg gctcggacgc actgtctatt tgccttattg cttggcgttc
      841 ctggcggagg gcttggcccg gcacggcgat cgagcagcgg cactggccgc actgcgggaa
      901 ggattggaaa cagcgcgtgc caccggagaa catatgtggg atgcggaact gcaccggctt
      961 accgggtccg tgctgctcgc cgaaaacaag ctcgacgagg gccaggcctc ccttcagcag
     1021 gcgatccgca tcgcccaagc ccaacgagcg aaatcgctgg aattgcgcgc cgcccgcgat
     1081 ctcgctcgcc tctggggcga acagggccgg cgcaccgatg cccgcgacct cctcaccccg
     1141 gtctacggct ggttcaccga gggcttcgac accgccgatc tgaaagaggc gaaggcgttg
     1201 ctcgacgagc tgacgtgatc ccgtcgtaag catggccggc ggtctggcgc gaacgaccgc
     1261 tttctggccg gtccgaccat ccgctggcgc ggcacgaatg tctcttgagg gtcgactgcg
     1321 gtcgatttca ccgttcaatg cgaatggctg ggacgggtgg aaagcggata ctcatgcatc
     1381 gcagcaagaa agctctattc gatcacgtcg tcggcgcgag cgactaataa gggcggcagt
     1441 tgcaggccga gttgccgtgc agttttttcg ttcacgacaa actcaaattc cgttgggagt
     1501 tggacaggca attcggcagg attagcacca cgtaaaatta gatcaacata tgatgctgcg
     1561 cctgcggtaa ccgtatcggc ttttcccccg tactcggctt ttatcccgta cgacataagg
     1621 ccgccggcct cgcagaagag gcgagccggg tagatcgcgg gtagcctgca ctgcgcaacc
     1681 agcgcagtaa tctgaacgtg ccgtgccccc gcgaacgcat cgggcagaaa aacgagccca
     1741 ctattcggct catccttgag cgagcgaata agacgatcaa tgtctgaact atcgccgctc
     1801 ggtgcttcaa ccgacacaat ccctactttg ttcgcgactt catcaattga tttgcgaaac
     1861 gccgaagaat tgttacccgg ctcagggtta tacactatcc c
//
LOCUS       DATLJT010001241         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1245009,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001241 DATLJT010000000
VERSION     DATLJT010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245009"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VM910_28540"
     CDS             <1..450
                     /locus_tag="VM910_28540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66496.1"
                     /translation="IIVVVSTLTIALLYAFMKFTFMGKALRAVAQDRETAGLMGVNPN
                     QLIMLTFVIASALAGMGGAMLGPILVLTPFAGTTVIVKAFAIVIIGGFGNVEGTIIAG
                     LVVGLIESYTTQFVNPGLTDIVVFTLLLLMLALRPTGLIAEKREENV"
     gene            473..1477
                     /locus_tag="VM910_28545"
     CDS             473..1477
                     /locus_tag="VM910_28545"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66497.1"
                     /translation="MLTDRVRISRRGWFLVVAVIIALLPFVIQDSYWRTNLVVCAINI
                     MLAIGLDFILGYAGQLNLGHSAFYGIGAYASTLLIMKLGVPFWMAFVLGMVLSGAAGM
                     ALSIFAVRLRGHYLAIASLGFAVIVHQVLLNWISLTQGPLGIYAIKPPPDIALPGLPV
                     ISFGNTANMFYLVASFAFLFYFLLDQLVRSPIGETLKAIREDEISASAFGINCTAWKV
                     FAFGIGSAVAGAAGAFYASFVGTLVPDAFIITESFTILAMVIVGGMGTLIGPVWGAIL
                     LTILPELLRGFGDLRLILYGAALTLVVLFMPGGIAQAAQMFCARLGRLATATDAGMRQ
                     "
     gene            1474..2280
                     /locus_tag="VM910_28550"
     CDS             1474..2280
                     /locus_tag="VM910_28550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66498.1"
                     /translation="MIASGPATAAQARPAGAGAFFCGAGLHKRFGGVQAVRDISLTVP
                     PGSVFAIIGPNGAGKSTLLNLMSGLYQPDAGRMIFDGVDLVGLPAHKRVRLGLGRTFQ
                     KIRLFKQLSVLGNVIAAFHIHHDIPAWQYVIHGAAFERDHARCRAEATKLLDFVGLEA
                     RASVLAASLAYGEQRMLELARALATSPRLLMLDEPAAGLNAADVERLVERIRILRKRG
                     MTIIVVEHNMDLVMKIADRVLVMDYGQYLFEGTPLEVQANSAVIAAYLGA"
     gene            2291..>2998
                     /locus_tag="VM910_28555"
     CDS             2291..>2998
                     /locus_tag="VM910_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66499.1"
                     /translation="MLQAEGLELAYGEVSACRGVSFHVAEGEIVTLIGANGAGKSSTL
                     RAVAGALLPRAGAIYFRGHDITRLPPYKRTELGISLVPEGRRVFPFLTVRENLELGAF
                     KYRKEGAKVRALMEKVFDMFARLRERQSQNAGTLSGGEQQMLALGRAIMSEPHLICLD
                     EPSLGLAPVVVQDIFRTIKAINAAGTSVLLVEQNARYALETASRGYVLQTGSIIASGS
                     CATLRNDDRVRQAYLGRS"
BASE COUNT          508 a          941 c          887 g          662 t
ORIGIN      
        1 atcatcgtcg tggtatccac cctcaccatc gcgttgctct acgcattcat gaaattcacc
       61 ttcatgggca aggcgctgcg cgccgtcgcc caggaccgcg agaccgcggg cctcatgggc
      121 gtcaatccca accaactcat catgctgacc ttcgtgatcg catcagcgct cgccggcatg
      181 ggcggcgcca tgcttgggcc catcctcgtg ctcacaccct tcgctggcac gaccgtgatc
      241 gtgaaggcgt ttgccatcgt gatcattggc gggttcggca atgtcgaggg gacgattatt
      301 gcagggctcg tggttggtct gatcgaaagt tatacgaccc agttcgtcaa tcccggccta
      361 accgacatcg tggtcttcac gctgctgctg ctgatgctcg cgttacgtcc gacagggctg
      421 atcgctgaaa agcgtgaaga gaatgtctaa cgaggctcga ccggcgtttc ccatgctaac
      481 cgatcgagtc cgaatcagcc ggcgcgggtg gttcctcgtg gtcgccgtga tcatcgctct
      541 tttgccgttc gtcattcaag attcttattg gcgcacgaac ctagtcgttt gcgcgatcaa
      601 catcatgctg gctatcggcc tcgatttcat actcggctat gccgggcagc ttaacctcgg
      661 tcactcggcc ttctacggca tcggcgccta tgcctctaca ctgctcatca tgaagcttgg
      721 cgtgccattc tggatggcgt tcgtcctcgg aatggtattg tccggcgcgg ccggcatggc
      781 attgtcgatc ttcgccgttc gtctgcgcgg ccattacctt gcgatcgcct cgctcggctt
      841 tgccgtcatc gtacatcagg tgctgctgaa ctggatcagc ctcacgcaag gcccactcgg
      901 catctatgcc atcaaaccgc caccggacat tgccttgccg gggctgccgg tcatctcgtt
      961 cggcaacacg gcgaatatgt tctatctggt ggcgagtttc gcgttccttt tctatttcct
     1021 gctcgaccaa ctcgtccgct cgccgattgg cgagacgctt aaagcgatca gagaggacga
     1081 gatttcggct tctgcgttcg gcatcaattg cacggcgtgg aaggtgtttg ccttcggcat
     1141 cgggtcggca gtcgcgggcg ctgccggcgc cttctatgcg agcttcgtcg gaacgttagt
     1201 cccagacgcc tttatcatca ccgaatcctt caccattctc gccatggtga tcgtcggcgg
     1261 catggggacg ctgatcggcc cggtctgggg cgcaatcctg ctcacgatct tgcccgaact
     1321 tttacgcgga ttcggcgatc tccgtctgat tctctatggc gccgccctca ccctcgtcgt
     1381 gctgttcatg ccaggaggga tcgcgcaggc agcgcagatg ttctgcgcgc ggttgggtcg
     1441 gctagcaacc gccactgacg ctggaatgcg ccaatgatcg cgtcgggccc cgccacggcg
     1501 gcgcaagcgc gtccggccgg tgccggcgcg ttcttttgcg gcgcaggctt gcacaagcgg
     1561 ttcgggggcg tgcaggcggt tcgcgacatt tcgctcaccg tcccgcctgg cagcgtgttt
     1621 gcgatcatcg gcccgaacgg ggccggcaaa tcgacgctgc tgaacctgat gagcgggctc
     1681 tatcagccgg atgcgggccg gatgatcttc gacggcgtcg acctcgttgg cttgccggcg
     1741 cacaaacgag tacgcctcgg cctcgggcgc acgtttcaga agatacgact ttttaagcag
     1801 ctatccgtgc tcggcaatgt catcgccgcc tttcatatcc atcacgatat cccggcctgg
     1861 caatacgtca tccacggtgc cgccttcgag cgcgaccacg ctcgctgtcg cgccgaggcc
     1921 accaagcttc tcgatttcgt cgggctcgaa gcgcgggcat cggtgctcgc agcttcactc
     1981 gcgtatggcg agcagcgcat gcttgaactt gctcgcgcac tcgcgacctc gccccggctg
     2041 ctcatgctcg acgagcccgc cgccggcctc aatgcggcgg acgtggagcg gttggttgaa
     2101 cggattcgta tccttcgtaa gcgtggcatg accattatcg tagtcgagca caacatggat
     2161 ctggtgatga agatcgcaga tcgcgtgctg gtcatggatt acggacagta ccttttcgag
     2221 ggcacgccgc tcgaggtcca ggcgaattcg gcggtgatcg ccgcctatct tggagcatga
     2281 cgtgagcgca ctgctgcaag cggaaggtct tgaactcgcc tatggcgagg tctcggcgtg
     2341 ccgtggtgtc agttttcatg tcgcagaggg cgagatcgtc acactgatcg gcgccaacgg
     2401 ggccggcaaa tcgagcacgc tacgcgctgt cgccggcgcg cttttgccac gcgccggcgc
     2461 gatctatttc cgcggacacg atatcacgcg cctcccaccg tataagcgca ctgaactcgg
     2521 aatatcgctt gttcccgagg gacgacgcgt ctttccattc ctcacggtgc gcgaaaatct
     2581 cgagctcggc gccttcaagt atcgcaaaga gggcgccaag gttcgcgccc tcatggaaaa
     2641 agtcttcgac atgtttgcac gattgcgcga gcggcagagc cagaacgctg gcaccctgtc
     2701 cggcggcgaa caacagatgc ttgcgcttgg gcgcgccata atgtcagagc cgcacctcat
     2761 ttgtcttgac gagccttcgc tcggtctcgc acccgtggtc gtgcaggaca ttttccgcac
     2821 gatcaaggcg atcaatgccg ccggcactag cgtgcttctg gtcgagcaga acgcgcgcta
     2881 tgcacttgag actgcgagcc gcggttacgt gctgcagacg ggatcgatca tcgcaagcgg
     2941 ctcctgtgcg acgttgcgca acgatgatcg cgtgcgccaa gcctacctcg gccgcagc
//
LOCUS       DATLJT010001242         1703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_556722,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001242 DATLJT010000000
VERSION     DATLJT010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556722"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="serB"
                     /locus_tag="VM910_28560"
     CDS             <1..835
                     /gene="serB"
                     /locus_tag="VM910_28560"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HVI66500.1"
                     /translation="ALAVLPAAGRPQWLFDEVAIDIPFESDLKSKDDIRTIEARLRAS
                     RGDLPIDIVVQPRTARRKKLFLADMDSTMIGQECIDELAAFVGLKDRVAEITERAMHG
                     EIEFEPALRERVALLKDLPVSVVDEVLAKRITLTPGGRELVMTMRAHGAYTCLISGGF
                     TVFTRPIAAKIGFQENRANELIARDGKFTGEVKEPILGRATKLATLIELTEDFDLDDI
                     DTLVVGDGANDLGMIQNAGLGVAYHAKPTVAATAAARIDHGDLTALLYTQGYTRQEFV
                     S"
     gene            complement(878..>1703)
                     /locus_tag="VM910_28565"
     CDS             complement(878..>1703)
                     /locus_tag="VM910_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVI66501.1"
                     /translation="INRGNSGGPLFNLDGEVIGVNTLIISPSGGSIGIGFAVPSKTVA
                     GVVSQLQQFGELRRGWLGVRIQTVTDEIAESLNIKPARGALIAGVEDKGPAKPAGIEP
                     GDVVIKFDGKDIKEPKDLSRVVADTAVGKEVDVVIIRKGAEETRKVTLGRLEDNEKAQ
                     LAAAKSKDEPADKPVTQKALGLDLAALSKDLRNKYKIKDSVKGVLITGVDGTSDAAEK
                     RLSAGDVIVEVAQEAVSSAADIKKRVEQLKKDGKKSVLLLVSNGDGELRFVALSVQ"
BASE COUNT          294 a          574 c          526 g          309 t
ORIGIN      
        1 cgcgctcgcc gtcttgccgg cggccgggcg gccgcaatgg ctgttcgacg aggtcgccat
       61 cgacattccc ttcgaaagcg atctcaaaag caaggacgac atccgcacca tcgaagcccg
      121 gctgcgcgcc tcacgcggcg acctgccgat cgatatcgtg gtgcagcctc gcacggcccg
      181 gcgcaagaag ctttttctgg cggacatgga ttccaccatg atcggccagg aatgcatcga
      241 cgagttggcc gcctttgtcg ggctgaagga ccgtgtcgcg gagatcaccg aacgggcgat
      301 gcacggcgag atcgaattcg agcccgcgct gcgcgagcgc gtcgcgctgt tgaaggacct
      361 gcccgtcagt gtggtcgacg aggtgctggc aaagcgcatc acgctgaccc ccggcggccg
      421 cgaactggtg atgaccatgc gcgcgcatgg cgcctacacc tgcctgatat cgggcggatt
      481 taccgtgttc acgcgcccga tcgcggcgaa gatcggcttc caggaaaacc gcgccaatga
      541 gctgatcgcg cgcgacggca agttcaccgg cgaggtcaag gagccgatcc tgggccgcgc
      601 gaccaagctt gcgacgctga tcgaacttac cgaagacttc gacctcgacg acatcgatac
      661 gctggtcgtc ggcgacggcg ccaacgacct cggcatgatc cagaacgccg gcctcggcgt
      721 cgcctaccac gccaagccga ccgtggcagc aaccgccgcc gcgcgcatcg atcatggcga
      781 cctcaccgca ttgctctaca cgcagggata cacgcggcag gagtttgtgt cgtgagtctc
      841 gcgtcatcac ccgcgaaagc gggcgatgac ggacgagtca ctgcacgctc agcgcgacga
      901 agcgcagttc gccgtcgcca ttggagacaa gcagcaagac cgacttcttg ccgtccttct
      961 tcaattgctc gacgcgcttc ttgatgtcgg cggcgcttga aaccgcctcc tgtgccacct
     1021 cgacgatgac gtcgccggcc gacagccgct tctcggcggc atccgaggtg ccgtcgacgc
     1081 ccgtgataag cacgcccttg acgctgtcct tgatcttgta cttgttgcgc aagtccttgc
     1141 tcagcgcggc gagatcgagc ccgagcgcct tctgcgtcac cggcttgtcg gccggctcgt
     1201 ccttggattt cgcagcggcg agttgagcct tttcgttgtc ttcgagccgc ccgagcgtga
     1261 ccttgcgggt ctcctcggca cctttacgga tgatgacgac gtcgacctcc ttgccaaccg
     1321 cggtatcggc cactacgcgc gacaggtcct tcggctcctt gatgtccttg ccgtcgaact
     1381 tgatcacgac gtcgccgggc tcgatccctg cgggcttggc cggtcccttg tcttcgacac
     1441 ccgcgatcag cgcgccgcgc gccggcttga tattgaggct ctcggcgatc tcgtcggtga
     1501 cggtctggat gcgcacgccg agccagccgc gacgcagctc tccgaactgc tggagttgcg
     1561 aaaccacgcc cgccaccgtc ttcgacggca cggcaaaacc gatgccgatc gagccgccgg
     1621 agggcgagat gatcagcgta ttgacgccga tcacttcgcc atcgaggttg aacagagggc
     1681 cgccggaatt gccgcggttg atc
//
LOCUS       DATLJT010001243         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3772659,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001243 DATLJT010000000
VERSION     DATLJT010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3772659"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VM910_28570"
     CDS             complement(<1..152)
                     /locus_tag="VM910_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVI66502.1"
                     /translation="MRRLRRIKILATLGPSSSDSAMIRRLFEAGADIFRINMSHTPHD
                     KMRDLVA"
     gene            complement(149..739)
                     /locus_tag="VM910_28575"
     CDS             complement(149..739)
                     /locus_tag="VM910_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HVI66503.1"
                     /translation="MMSHATVRRHSTAAWLGLLPALLVLSLWSNSAWADFRLCNNTSS
                     RVGIALGYKDAEGWTTEGWWNISSRSCETLLKGTLVARYYYIYALDYDRGGEWSGQAF
                     MCSRDKEFTIKGTENCLARGFDRTGFFEVDTGDQRAWTVQLTDANEQPGQQRVPGIPG
                     PVGPGGSVLGLPNTPGGAAPPGASGLPPAAAPGSKP"
     gene            935..1237
                     /locus_tag="VM910_28580"
     CDS             935..1237
                     /locus_tag="VM910_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HVI66504.1"
                     /translation="MAIDDKTRTELEAAAFRRLVDHLRERTDVQNLDLMNLAGFCRNC
                     LSNWLKDAADAKGVAMSKDESREAVYGMPYETWKSKYQGAATPEQLAAMKKAHPGH"
     gene            1315..1467
                     /locus_tag="VM910_28585"
     CDS             1315..1467
                     /locus_tag="VM910_28585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66505.1"
                     /translation="MIRTVVAVALFASGIVVGVSSSQILHAQAPTYATKEIIRTDLNN
                     LPGRRR"
     gene            1467..1730
                     /locus_tag="VM910_28590"
     CDS             1467..1730
                     /locus_tag="VM910_28590"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66506.1"
                     /translation="MFASTWQPGFRLPWHMHPDGHEFTFVVEGEQTFEIEGVGTKVVK
                     AGEAIYTPPNTPHYGRNATDKESKTVVVRIKAKNQPVMVELKR"
     gene            1894..2163
                     /locus_tag="VM910_28595"
     CDS             1894..2163
                     /locus_tag="VM910_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HVI66507.1"
                     /translation="MATSAAVKEAPATKFAKDQLKAIIERIERLEEEKKTISDDIRDV
                     YAESKGNGFDVKALRTIVRLRKQDPNERQEQETILETYMQALGML"
     gene            complement(2281..>2961)
                     /locus_tag="VM910_28600"
     CDS             complement(2281..>2961)
                     /locus_tag="VM910_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI66508.1"
                     /translation="LASADADVVPPLKAKPAAETAQSKPETPADIINARGFWDDGADA
                     PKQATPEQLVAAVSARRTREAADPQPTASVPAAYQAMAYVPDPSSPVDHASPIMASAP
                     IPRSVRPAVSRNAAAATGINTIATKGSQGQDELVANSTRLSAAKGDELWMRIMMLAPS
                     AVTMSVTVIGDSDLTLMRAFFVKPPASVTMSFSDDPMPGLSTDRFSGSAATKLDITSF
                     TMRTALLR"
BASE COUNT          585 a          873 c          973 g          530 t
ORIGIN      
        1 gcgaccagat cccgcatctt gtcgtgcggg gtgtggctca tgttgatgcg gaaaatgtcg
       61 gcgccggcct cgaacaggcg acggatcatc gcgctgtctg acgacgacgg acccagggtc
      121 gcgagaatct tgatccggcg cagacgcctc atggcttgct tccgggcgct gcggctggcg
      181 gcaggccaga cgcgcccgga ggcgccgcac cacccggcgt attgggcagg cccaggacgc
      241 tgcctcccgg ccctaccggt cctggaatgc ccgggacgcg ttgctgcccc ggctgctcat
      301 tggcgtcggt caattgcacc gtccaggcgc gctgatcgcc ggtgtcgacc tcgaagaagc
      361 ccgtgcggtc aaagccccgc gccaggcaat tttcggtgcc cttgatggtg aactccttgt
      421 cacgagagca catgaaagcc tgccccgacc actcgccgcc acgatcgtag tcgagcgcat
      481 aaatataata gtagcgggcg accagtgttc ccttgagcag cgtttcgcag cttcgcgagg
      541 agatgttcca ccagccctcc gtggtccacc cctcggcatc cttgtagcct agggcaatgc
      601 ccacccggct cgacgtgttg ttgcaaaggc ggaagtcggc ccacgcagaa ttcgaccaca
      661 ggctgagcac caggagagcc ggcagcaggc cgagccaggc ggcggtcgaa tggcggcgga
      721 ctgtagcgtg agacatcatg gcgcggagga gtgtcaattg gtccgagatt tcgcgtaagg
      781 gggaagcgac tgtcaacggc cagggcgctg ctcaaggcgc cgatttcggc ccgtccgcca
      841 ccagacccat aaatcattgt ctgggttagg atatggcaaa atctgtgaca aggtccacat
      901 gatagggcag ctctgcaacg gcgggataac gatcatggcc atcgatgaca aaaccagaac
      961 ggaacttgaa gccgccgctt tccggcgtct tgtcgaccat ttgcgcgagc ggaccgatgt
     1021 ccagaacctc gatctgatga acctcgccgg cttctgccgt aactgtctgt ccaactggct
     1081 caaggatgcg gcggatgcaa agggtgtcgc catgagcaag gacgagagcc gcgaggcggt
     1141 ctacggcatg ccctatgaga cctggaagtc gaaatatcag ggcgcggcca cccccgagca
     1201 actcgcggcg atgaagaagg ctcacccggg acactgagtg ggggcttgcc cggaatgcgc
     1261 ggacagggtt cgctgctcca gtcgtcggta tcttcgccag actggagcag gttgatgatc
     1321 aggactgttg tcgccgtcgc gctgtttgcg agtggaattg tcgttggggt ttcctcttcc
     1381 cagatcctcc atgcccaggc gccgacctac gcgaccaaag agatcatccg aacggacctg
     1441 aacaatctgc ccggcaggag gcgctgatct tcgcgtcgac gtggcagccc ggcttcaggt
     1501 tgccatggca catgcatccg gacgggcatg agttcacatt cgttgtcgaa ggcgagcaga
     1561 ccttcgagat cgaaggcgtc ggcacaaagg tcgtgaaggc gggcgaagcg atctatacgc
     1621 cgccgaatac gccgcactac ggccgcaacg cgaccgacaa ggaatcaaaa acggtggtgg
     1681 tccgcatcaa ggccaagaat cagccagtca tggtcgaatt gaagcgctag caagaggcga
     1741 gacttcgcga gcgcatctcc tgtgggcgcg ctgtggatga gcgcgatgtt tccttgaccc
     1801 gagccgctcg aacttcgagt gtcgtttcaa cgtatcgtgc gcgaaagcgt gcgcgacgaa
     1861 gttcaatgtc agttccattt aggagtttaa gggatggcca cctccgccgc cgtcaaggaa
     1921 gcgcccgcga cgaaatttgc aaaagatcag ctcaaggcca tcatcgagcg cattgagcgg
     1981 ctggaagaag agaagaagac gatctccgac gatatccgcg acgtctatgc cgagagcaag
     2041 ggcaatggtt ttgacgtgaa ggcgttgcgc acgatcgtgc gcctgcgcaa gcaggatccc
     2101 aacgagcgcc aggagcagga gaccatcctt gaaacttata tgcaggcgct ggggatgctg
     2161 tagggcgata ctgtagacgg cgctggaaaa ggcggcatcg cgacgccgtc atcgcttctt
     2221 cgtctctcct cttgttggag agagtgagcg aattcgaaaa accctggagg ttaaagcggc
     2281 tcagcgcaat agcgcggtgc gcatcgtgaa cgacgtgata tcgagcttcg tggccgcaga
     2341 gcccgagaag cggtcggtgc tcaggcccgg catcggatcg tccgaaaagc tcatggtgac
     2401 agaagccggc ggcttgacga agaacgcgcg catcaaggtc agatcgctgt cgccgatgac
     2461 cgtcaccgac atcgtgacgg cgctcggcgc cagcatcatg atgcgcatcc acagctcgtc
     2521 gcccttggcc gcagagagcc gggtcgagtt ggcaaccagt tcgtcctggc cctgcgatcc
     2581 cttggtcgcg atggtattga tcccggtcgc ggcagccgcg ttgcgcgaaa ctgccgggcg
     2641 cacgctgcgc gggatcggcg cggaggccat tatggggctt gcgtggtcaa ccggcgacga
     2701 agggtcgggt acgtaggcca tcgcctgata ggccgcgggc acgctcgcgg tcggctgcgg
     2761 atcggcggcc tcacgggtcc ggcgcgcgct gaccgcggcg accagttgct ccggcgtggc
     2821 ctgtttgggc gcatctgcgc cgtcatccca gaagccgcgg gcattgatga tgtcggccgg
     2881 ggtttccggc ttcgactggg cggtctcggc agcgggcttc gccttgagtg gcgggactac
     2941 gtcggcgtcg gccgatgcga g
//
LOCUS       DATLJT010001244         6493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2393628,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001244 DATLJT010000000
VERSION     DATLJT010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393628"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VM910_28605"
     CDS             <1..628
                     /locus_tag="VM910_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HVI66509.1"
                     /translation="AQDAATLKKNMIGQWELSTTERSKTCVVTLKPDATPQGLKLELE
                     PGCPKALPFTKDITAWNIKGLDIVRLQDATGEAVIDFTEVEAGILEGLRSGEGVYILQ
                     NLAAARSLAKSMDQMIGDWAMVRGNGNTICGLTLTNNDAGNENFQVFLKPKCDPAIAS
                     FAPTQWRLERGQIMLMSDRGDTWQFEADDNAQWRLVPDTADPLIMVRQ"
     gene            673..1047
                     /locus_tag="VM910_28610"
     CDS             673..1047
                     /locus_tag="VM910_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66510.1"
                     /translation="MAKSVLAIGIDPAFLNFSSFPQFTPEMFRQHIDSQIDRVRALGY
                     DVTLCLVDLGDTAEAVTATALGAGTFDCVLIGAGLREGREHVVLLEKIVNLVHRLAPT
                     AAICFNTTPSDTAEAVQRWIEP"
     gene            complement(1152..2024)
                     /locus_tag="VM910_28615"
     CDS             complement(1152..2024)
                     /locus_tag="VM910_28615"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66511.1"
                     /translation="MRRRDFIAVLGGAAAAWPLAARAEQRPMPLMGLLTLAKPEWVMN
                     AIQDGLREAGFVDGRNLTMIARSADGQFDRLPALAAELVRSRVDVIMATGSPVPARAA
                     KAATTQIPIVFAYGGDPVADGLVNSMNQPGGNVTGATFIGSSLLAKRMELLREIVPQA
                     SDVALLVNPKGTLAERQIKDGTAAAATLGQRLHVINTSTVNDIDAAFETMVQSKIGAF
                     IVSTDPFFGFIGRDRLVNLARRYKIPGIYNAREESGIGGLVSYGPNLPDTWRQAAVYV
                     GRILKGEKPRSKSS"
     gene            2193..2585
                     /locus_tag="VM910_28620"
     CDS             2193..2585
                     /locus_tag="VM910_28620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66512.1"
                     /translation="MSALTVFLARLLGLFTVLIVIALVFRGPAVVEAAVADQPVMLTY
                     GMISLAIGLGMVLGHNVWSGGLLPVVVTLVGWLILAKGLLLLALAPDTLAHLYGRMHR
                     GESLALYIPSLVIGLYLTVAGFAVPSRS"
     gene            2867..3172
                     /locus_tag="VM910_28625"
     CDS             2867..3172
                     /locus_tag="VM910_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HVI66513.1"
                     /translation="MIRQVGIAALLALATAMPAHEALAQNALGGAVLGGVGGAIVGGA
                     IGGGRGAAIGAVVGAGTGAAIAAEGERRRSGYYYYHNGCYLQRPDGAWVLVGPRYCY"
     gene            complement(3254..3965)
                     /gene="radC"
                     /locus_tag="VM910_28630"
                     /pseudo
     CDS             complement(3254..3965)
                     /gene="radC"
                     /locus_tag="VM910_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604951.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(4031..4852)
                     /gene="map"
                     /locus_tag="VM910_28635"
     CDS             complement(4031..4852)
                     /gene="map"
                     /locus_tag="VM910_28635"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVI66514.1"
                     /translation="MSYIEATDTSLRKTGQIKLHGASGFAGMRKAGALVAKCLDELTD
                     IVKAGVPTSKVDEFVREFAFSHGAYPATLMYRGYRYSTCTSINHVVCHGMPGDRGLKE
                     GDIVNIDVTFIVDGWYGDSSRMYAIAPISRKAERLIEVTYEAMMRGIAAVKPGATTGD
                     IGHAIQSYVEPQGMSVVRDFCGHGLGRLFHDEPNIIHIGRPGEGVPLKPGMFFTIEPM
                     INLGKPHVKILSDGWTAVTRDRSLSAQFEHSVGVTATGVEIFTLSERHGEKPPAT"
     gene            complement(4980..6296)
                     /locus_tag="VM910_28640"
     CDS             complement(4980..6296)
                     /locus_tag="VM910_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVI66515.1"
                     /translation="MEMDAKDILDFLHAAARSVGAEVTSPWFYFQLGLILVGAGLAFA
                     VNAIVHARIDTSSFAKDWPAPLRLFVRTLIGSTAATVFAVMMVLERAAMVAATWPSRS
                     YLLAVAAKLALAWLIIRLVTSGIRNTFVVRLVSVMAWLAAALSILGKLEPTIEALDSV
                     SIVLGGFKLTPLLLLNFAVILIIALWLANVISNFAESRITQSADLTPSIQVLLVKMIR
                     LGLLVFAVIVAMGAVGINLSALAIFSGAAGVGIGFGLQKIVANFISGIILLVDKSVKP
                     GDLVTIGDNSGRISAMKTRYISVAAGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKVN
                     FATNYDADPRLVCRVAIEIAAGAARALKNKPPNCILTEFAEAGMKFSLTFWIADPDGM
                     DNVKSEVMLSLWDAFKREGIRVPYPVREIRVRGGTLPVETSVEVSN"
BASE COUNT         1184 a         1982 c         2094 g         1233 t
ORIGIN      
        1 cgcgcaggat gccgcgacgc tgaagaagaa catgatcggg cagtgggaac tgtcgacgac
       61 cgagcgcagc aagacctgcg tggtcacctt gaagccggac gccacaccgc aaggcctcaa
      121 gctcgagctc gagcccggct gccccaaggc attgcccttc accaaggaca tcaccgcctg
      181 gaatatcaag gggctcgaca tcgtgcggct gcaggacgca acaggcgaag ccgtgatcga
      241 tttcaccgaa gtcgaagccg gcattctgga gggcctgcgg agcggcgagg gggtctatat
      301 cctgcaaaat ctcgcggcgg cgcgttcgct tgccaaatcg atggatcaga tgatcggcga
      361 ctgggcgatg gtccgcggca atggcaacac gatctgcggg ctgacgctga ccaacaacga
      421 tgccggcaac gagaatttcc aggtctttct gaagccgaaa tgcgatccgg cgatcgcgtc
      481 ctttgcgccg acgcaatggc ggctggagcg aggccagatc atgctgatgt cggatcgcgg
      541 cgacacctgg cagttcgagg ccgacgataa tgcgcagtgg cggctcgtgc ccgacaccgc
      601 cgatccgctg atcatggtgc ggcagtagtg gcgcgcggcg cttgagcctg cgtgcgcacg
      661 ggagcaagct ccatggcgaa gagcgtactc gcgatcggca tcgatccggc attcctgaac
      721 ttttcctcgt ttccgcaatt cacgccggag atgttccgcc agcacatcga tagccagatc
      781 gaccgcgtcc gtgcgcttgg atacgacgtc acattgtgcc tcgtcgatct cggcgacacc
      841 gccgaggcgg tgaccgcgac cgcgctcggc gccggcacgt ttgattgcgt tctgatcggc
      901 gccggtctgc gcgagggacg cgagcacgtc gtcctgttgg aaaagatcgt caacctcgtt
      961 caccggctcg cgccaacggc ggcgatctgc ttcaacacga cgccatcaga cacggccgag
     1021 gccgtgcagc gctggatcga gccataggac ctgtgccgca aggacatgct cggcctattc
     1081 gatcacctcg tcggcaagac ctagcagcgc aggcgggatc gtcagtccga gctcccttgc
     1141 cgccttcaga tttatgacga tttcgaacga ggtttttcac ctttcaggat gcggccgaca
     1201 tagacggccg cctgccgcca ggtatcaggc aaattggggc catagctgac gaggccgcca
     1261 ataccggatt cctcgcgcgc attgtagatt ccggggattt tgtagcgccg tgcaaggttc
     1321 accagacggt cacggccgat gaagccgaaa aacggatcgg tgctgacgat gaatgcaccg
     1381 atcttggatt ggaccatggt ctcgaacgcg gcatcgatgt cgtttacggt gctggtgttg
     1441 atgacgtgaa gacgctggcc aagcgtcgcg gcggcggccg tcccgtcctt gatctggcgc
     1501 tccgcaagcg ttcccttcgg attcaccagg agggcgacgt cgctcgcctg cggcacgatt
     1561 tcccgcaaca gctccatgcg ctttgcaagc agcgagctgc cgatgaaagt tgctccggtg
     1621 acgttgccgc cgggctggtt catgctgttg accagaccgt cggcgaccgg atcgccgcca
     1681 taggcgaaga cgatcggaat ttgcgtggtc gccgcctttg cggctcgcgc cgggaccggc
     1741 gagcctgtcg ccatgatgac atcgacccgg ctccgtacca actcggccgc gagtgcgggc
     1801 agccggtcga attgaccgtc cgccgagcgc gcgatcatgg tgaggttgcg accgtcgaca
     1861 aagccggcct ctctcaagcc gtcctgaatg gcattcatga cccactcggg cttcgccagc
     1921 gtcagcagtc ccatcagtgg catcggccgc tgctcggccc gcgccgcaag cggccaggcc
     1981 gcggctgcgc caccgagaac tgcaatgaag tcgcgtcgtc tcattctttc cgtcgggaat
     2041 ccggaacccc gctcgtctat atcaagccgg ctcccgttgt gccacccgcg gcacggcaat
     2101 ggcgcgatgc ggtgcccgat tgctgcagat caacgagcgg cagcggtcat aagctagtct
     2161 cgtgtgagtc cacgacagac tggagatttg ccatgtcagc gctcacggtt tttctcgccc
     2221 ggcttctcgg cctgttcacc gtcctgatcg tcatcgcgtt ggtgtttcgc ggtcctgccg
     2281 tggtcgaagc ggccgtcgcc gaccagccgg tcatgctgac ctacggcatg atcagcctcg
     2341 ccattggtct tggcatggtg ctcgggcaca atgtctggtc gggcggcttg ctcccggtgg
     2401 tggtcacgct tgtcggctgg ttgatcctgg cgaaaggact cctgctgctc gcgctcgccc
     2461 cggacacgct ggcccatctc tatggacgga tgcatcgcgg cgaaagtctc gcgctttaca
     2521 tcccctcgct cgtgatcggg ctttatctga cggtagcggg ttttgccgtg ccttcgcgca
     2581 gctagcgtca atgccgcaag ctgcgcctcg gccgagttgc gtcagtgcgc cgccgcggca
     2641 ttagggatga tccgaagcag cgggtccgta gcaatggtga tgtttggtcg catggtcgat
     2701 gcggccggtt caaaacggag agcggaaccg ccgggccgat gaccaaacct gtgccgaggt
     2761 caagtccctt ctgatcatgc ggcatctgtt cccgctcgtg cagccgattg acttccaggg
     2821 aagcgattgc gaacaagcga acgtctacga accggagatc gatggaatga ttcgacaggt
     2881 tggaattgcg gcgttgctgg ccctcgctac ggccatgccg gcccacgagg cgcttgccca
     2941 gaatgcgctt ggcggcgccg tattgggagg tgtgggcggg gcgatcgtcg gtggcgcaat
     3001 cggcggcggt cgcggtgcgg caatcggtgc ggtcgtcggc gcaggaaccg gcgctgcaat
     3061 tgccgccgag ggggaacggc gccgcagcgg ctattactac taccacaacg gctgctacct
     3121 gcagcggccg gacggtgcgt gggtgctggt cggtccccgc tattgctact gagcttcatc
     3181 gccaagtcga acaagtcggg cggcgtccgc gcagcttgag cgcgatggct tcaggtcgag
     3241 tcgccatccc gccctagatc agtcgcattc ccttcaggct cgcgtgcccg cctcggccta
     3301 cgatgacgtg gtcgtgcacg gcaatgccga gcggcgttgc gatcgcgatg atcgccttgg
     3361 tcatctggat atcggcctgc gacggggtcg gatcgccgga cgggtgattg tgcaccagga
     3421 tcagcgcggt cgccgacaat tccagcgcgc gcttgatcac ctcgcgcgga tagaccggag
     3481 tatgatcgac ggtgccggtc tgctgcactt catccgcgat caactgattg cgtttgtcga
     3541 gaaacagcag gcgaaactgc tccttgtcgg caaacgccat gctggaggca atagtcgatt
     3601 acgtcgttcc acgacgacaa ttgcctgcgc tcccgcatct cgccgcgcgc gatgcgggtc
     3661 gcggccgccg cgatcagctt gagctgatgg atcgaggcgt ccttgatccc cttgatctcg
     3721 cgaagccgcg cttccggagc gtgaacgact tcggcgaagg agccgaaggc cttcaagagc
     3781 gatttggcaa gcggcttggt gtcgcgccgg ggcagcgcgg ggaacagcgc catttccagg
     3841 agttcgtagt cgctcagggc atcgggcccc gcgctgtaga agcgctcgcg cagccgctcg
     3901 cgatggccgt ggtagtgcgg ctcgtctgcg ggctcgtcgt ccggtggatc agtcctggcg
     3961 ggcatcggcg gcaagcatgc cctgcgccgc aacttttgca accacgaagt gtgcgttggt
     4021 ccgccggcaa ttacgtcgcc ggcggcttct cgccgtggcg ttcggacaac gtgaaaattt
     4081 cgacgccggt tgcggtcacg ccgaccgaat gctcgaactg cgccgacagc gagcggtcgc
     4141 gcgtcacggc ggtccagccg tcggagagaa ttttcacgtg cggcttgccg agattgatca
     4201 tcggctcgat ggtgaagaac atgccgggct ttaacggcac gccctcgccg ggccggccga
     4261 tatggatgat gttgggctcg tcatggaaca gccggccgag gccgtggccg cagaagtcgc
     4321 gcaccacgct cataccctgc ggttcgacat agctctggat ggcgtggccg atgtcgccgg
     4381 tggtggcacc gggcttcacc gcggcgatcc cgcgcatcat cgcctcatag gtcacctcga
     4441 tcagccgctc ggctttgcgc gagatcggcg cgatcgcgta catccggctg gaatcgccat
     4501 accagccgtc gacgatgaag gtgacgtcga tattgacgat gtcgccttcc ttcagcccac
     4561 ggtcgcccgg catgccgtgg cagaccacgt gattgatcga ggtgcaggtc gaatagcgat
     4621 agccgcgata catcagcgtc gccggatacg cgccatggct gaaggcgaac tcgcggacga
     4681 attcgtcgac tttggaggtc ggcacgcctg ctttgacgat gtcggtgagt tcgtcgaggc
     4741 atttcgccac cagcgcgcct gccttgcgca tgccggcaaa accgcttgcg ccatgcagct
     4801 tgatctgtcc ggtcttgcgg agggaggtgt cggtggcttc gatgtagctc atatcggggt
     4861 cggccttggc cgtcctgtcc cggacggtcg gtaaaattgc gggattttcc agtgatttga
     4921 aagctctaat ctaatgattg ggacggccgg cgcaagccga cctttgcctg ctctttgcct
     4981 taattggaga cttccacgct ggtctcgacc ggcagtgtgc cgccgcggac gcggatttcg
     5041 cgcaccggat agggcacgcg gatgccctcg cgcttgaagg catcccagag cgacagcatc
     5101 acctcgctct tcacattatc catgccgtcg ggatcggcga tccagaaggt cagcgaaaat
     5161 ttcataccgg cttccgcgaa ttcggtgagg atacagttcg gcggcttgtt cttgagcgcg
     5221 cgggcagcgc ctgcggcgat ctcgatcgcg accctgcaga cgagccgcgg atcggcgtcg
     5281 taattggtcg cgaaattgac cttcaccagc gtgttcttgt cggtgtaggt ccagttggtg
     5341 accttctgcg tcaccaggtc ctcgttcgga atcaggaact cgcgtccgtc gccggcagcc
     5401 accgagatat agcgggtctt catcgcgctg atccggcccg aattgtcgcc gatggtgacg
     5461 agatcgccgg gcttcaccga tttgtcgacc agaaggatga tgccgctgat gaagtttgcg
     5521 acgatcttct gcaggccgaa gccgataccg acgcctgcgg cgcccgagaa gatcgcaagt
     5581 gccgacaggt tgatgccgac cgcgcccatg gcgactatga cggcgaaaac aagcaggcca
     5641 aggcggatca tcttgaccag cagcacctgg atcgacggcg tcaggtcggc agactgggtg
     5701 atgcggcttt cggcgaaatt gctgatgacg ttggcaagcc acagcgcgat gatcaggatc
     5761 acggcgaaat tgagcaagag cagcggcgtg agcttgaagc cgccaagcac gatcgagacg
     5821 gagtcgagcg cttcgatggt cggctcgagc ttgccgagga tgctcagcgc tgcggcaagc
     5881 cacgccatca ccgataccag gcgcaccacg aacgtattgc ggatacccga cgtgacgagg
     5941 cggatgatca gccatgccaa ggcgagcttt gccgcaaccg ccagcagata gctgcggctc
     6001 ggccaggtgg cggcgaccat ggccgcgcgt tccagcacca tcatcacggc aaacacggtt
     6061 gccgccgtgc tgccgatcaa tgtccgcacg aacagacgca acggagcagg ccagtccttc
     6121 gcaaaggaag aggtgtcgat ccgcgcgtgc acgatggcgt tgaccgcaaa tgccagtccc
     6181 gcgcctacga ggatgaggcc cagttgaaaa tagaaccagg gcgaggtgac ctccgcgccg
     6241 accgagcgcg ccgccgcatg caggaaatcg aggatgtctt tggcgtccat ttccatcgtc
     6301 aagagcctca tggggccaat tcgattcgca atttcgaaag attccataac tgagcgatcg
     6361 atgccatcgg cagggcaagc cggcaggcga gatcaggccg gaaaatcggg cacattggcg
     6421 cagcgcccag aaatgggttg gcgcggaagt atggcgacga tatggatgca aacgcagcga
     6481 ttttgccaag ctc
//
LOCUS       DATLJT010001245         1728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4231315,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001245 DATLJT010000000
VERSION     DATLJT010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4231315"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1728)
                     /gene="mfd"
                     /locus_tag="VM910_28645"
     CDS             complement(<1..>1728)
                     /gene="mfd"
                     /locus_tag="VM910_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HVI66516.1"
                     /translation="AMLAVLGMESGFETDDVAVISEQDILGDRLVRPRKATRKLDNFI
                     SEVTSLASGDLVVHVEHGIGRFVGLQTLEVSGAPHDCLELHYAGETKLFLPVENIELL
                     SRYGSEQTNVELDRLGGSGWQTRKARLKNRIREIAGELIKIAAERMLHEAPKLAVQAG
                     LYDEFCARFPYEETEDQDVAIGATLKDLEAGRPMDRLICGDVGFGKTEVALRAAFAVA
                     LDGKQVAVVVPTTLLARQHARTFAERFKGFPVKVAQASRLVAQKELAQVKKGLADGSI
                     DIVIGTHALLGKSIKFRDLGLLIVDEEQHFGVSHKERLKQLRAEVHVLTLSATPIPRT
                     LQLALTGVRELSIIASPPVDRLAVRTFVAPHDPLMIREALLRERYRGGQAFYVAPRIE
                     DLAGVKDFLDKNVPEMKVAVAHGQMAPGMIEDIISAFYDGKYDILLSTTIVESGLDIP
                     NANTLIVHRADMFGLAQLYQLRGRVGRSKLRAYALFTLPAQQKITAQAEKRLTVLQSL
                     ETLGAGFQLASHDLDIRGAGNLLGEEQSGHIKEVGFELYQSMLEEAIINLKAGIAEPP
                     PERWSPQITV"
BASE COUNT          310 a          546 c          551 g          321 t
ORIGIN      
        1 gaccgtgatc tgcggcgacc agcgctcggg aggtggttcg gcgatgccgg ccttgaggtt
       61 aatgatcgcc tcctccagca tcgattgata aagctcgaag ccgacctcct tgatatgtcc
      121 cgactgctcc tcgccgagga gattgccggc gccgcgaatg tcgagatcgt gcgaggcgag
      181 ctggaaaccc gcgcccaggg tctccagcga ttgcagcacg gtcagccgct tctcggcctg
      241 tgcggtgatc ttctgttgcg ccggcaaagt aaacagcgcg tacgccctga gcttggagcg
      301 tccaacccgg ccgcgcaatt gatagagctg cgcaaggccg aacatgtcgg cgcgatgcac
      361 gatcaacgtg ttggcattcg ggatatcgag gccggattcg acgatcgtgg tcgaaagcag
      421 gatatcgtat ttgccgtcat agaacgccga gatgatatcc tcgatcatgc ccggggccat
      481 ctggccatgg gcgacggcga ccttcatctc cggcacgttc ttgtcgagga agtccttcac
      541 gcctgcgagg tcctcgatgc gcggtgcgac atagaacgct tgtccgccgc ggtagcgctc
      601 gcgcagcagc gcctcgcgga tcatcagggg atcgtggggc gcgacgaagg tgcgcaccgc
      661 cagccgatcg acgggtggcg aggcgatgat cgagagctcg cgcacgccgg tcagggcgag
      721 ttgcagcgtg cgcgggatgg gcgtagcgct cagggtcagc acgtgcacct cggcgcgaag
      781 ctgcttgagg cgctccttgt ggctgacgcc gaaatgctgt tcctcgtcga cgatcagaag
      841 gccgaggtcg cgaaacttga tcgacttgcc cagcaacgcg tgcgtaccga tgacgatgtc
      901 gatcgagccg tcggcaagtc ccttcttgac ctgcgccagc tccttctgag caaccagccg
      961 cgaagcctgc gcgaccttga cagggaagcc cttgaaacgc tcggcaaacg tcctggcgtg
     1021 ctggcgcgcg agcagcgtgg tcggcaccac caccgcgacc tgcttgccgt cgagcgctac
     1081 cgcaaaggct gcgcgcaacg cgacctcggt cttgccaaag ccgacgtcgc cgcagatcag
     1141 ccggtccatc ggtcggccgg cttcgaggtc tttcagggtt gcaccgatcg caacgtcctg
     1201 atcctcagtc tcctcgtagg gaaaccgcgc acagaactcg tcatatagtc ccgcctgcac
     1261 ggcgagcttc ggcgcttcat gcagcatgcg ctcggcggcg atcttgatca attcgccggc
     1321 gatctcgcgg atgcggttct tgagcctggc tttgcgcgtc tgccagccgc tgccgccaag
     1381 acggtccagc tcgacattgg tctgctcgga accgtagcgc gacagcagtt cgatgttctc
     1441 gaccggcagg aataacttgg tctcgccggc gtaatgcaat tcgaggcagt cgtgcggcgc
     1501 gcccgagacc tccaaggtct gcagtccgac gaaacggccg atgccgtgtt cgacgtggac
     1561 cacgagatcg cccgaggcca ggcttgtaac ttccgagatg aaattatcga gcttgcgggt
     1621 cgccttgcgc ggccgcacga gacggtcgcc cagaatgtcc tgctcgctga tgacggcaac
     1681 gtcgtcggtc tcgaaaccgc tttccatgcc gagcaccgcc agcatggc
//
LOCUS       DATLJT010001246         1682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_326462,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001246 DATLJT010000000
VERSION     DATLJT010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326462"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VM910_28650"
     CDS             complement(<1..1514)
                     /locus_tag="VM910_28650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66517.1"
                     /translation="MLKQTSTSAQDAALFAEMLSLPNDGRYPAVELTAQQRRQRTLEA
                     LVSQVEALARSTPVLMVFEDAHWSDPTSLEALGRAVDRIRRLSVLLIVTFRPEFEPGW
                     IGQPHVTPLSLNRLASREVGAMIDHIVGNNPLLANLRQDIIERTDGIPLFVEEMTKAV
                     LEAESEGEARRAAAFVPSPALAVPATLHASLMARLDRLGPGKELAQIGAAIGREFSHP
                     LLAAVVCKPEAKLQTALDRLIGAGLLLRQGSAPYATYFFKHVLVRDAAYSTLLREPRR
                     ALHARIAETLESQFPEISETQPELLARHCTEAGQIEKAAALWGKAGLRSAQRSALVEA
                     AEQLRRALDQIATLTATPALRREEIKLQVALITPLLHVNGYAAPETRAAVERARLLIE
                     QAEALGEPPEDPLLLFSVLYGFWVANLVAFKGDVMRELAVQFLALADKQKATGPLMMG
                     HRLMGLSLLHTGDIVDGRAHLDCAITFYDPAEHRPLATRFGQDIGAATLSWKSLG"
BASE COUNT          309 a          536 c          528 g          309 t
ORIGIN      
        1 cctaacgact tccaggacaa ggttgccgcg ccgatgtctt ggccaaaacg cgtcgccaga
       61 ggacgatgct cggcaggatc gtagaacgta atcgcgcaat ccagatgcgc tcgcccgtct
      121 acgatgtccc ctgtatgcaa caaggacaag cccatgaggc gatgccccat catgaggggc
      181 cccgtcgctt tttgcttatc tgcaagggcc aggaactgga cagcaagctc acgcatcacg
      241 tcgcccttga acgctacaag gttcgcgacc caaaagccgt aaagaaccga gaacagcaac
      301 agtggatctt caggaggttc tccgagcgct tcagcttgtt cgattagcag acgcgcccgc
      361 tccacggccg ccctggtttc cggcgccgcg tagccgttga catggaggag tggggttatg
      421 agtgcaacct gaagcttgat ttcttcgcga cgcagggcgg gcgtcgcggt caaggttgcg
      481 atctggtcga gcgcacgcct gagctgttcc gcggcttcga ccagtgccga gcgctgcgcc
      541 gaccgcagtc cggctttgcc ccacagcgcc gcggctttct cgatctgccc cgcctcggtg
      601 cagtgacgcg ccaacagctc gggctgggtc tcggagatct ccgggaactg actttcgagg
      661 gtttcggcga ttctggcatg gagcgcacgt ctcggctcgc gcagcagcgt gctgtaggcc
      721 gcatcccgta ccagtacatg cttgaaaaag taggtcgcgt acggcgcgct accctgccgg
      781 agcagcaaac cagccccaat gagacggtcg agcgccgttt gcagcttcgc ctccggcttg
      841 cacacgaccg cagccagtag gggatgggag aactcgcgtc cgatcgccgc cccgatctgc
      901 gccaactcct tgccggggcc aagccggtcg agccgcgcca tcaacgatgc gtgcaaggtt
      961 gcggggaccg ccagtgccgg cgaaggaacg aaggcggcgg cccgccgcgc ttcgccctca
     1021 ctctctgcct ccagcaccgc cttcgtcatc tcttcgacga acagggggat gccgtccgtg
     1081 cgctcgatga tatcctgccg taggttcgcc agcagcggat tgttgccgac gatgtgatcg
     1141 atcatagcgc cgacttcgcg agacgccagc cgattgaggc tgaggggagt cacgtgcggc
     1201 tgtcctatcc agcccggctc gaattccggc cggaaggtta cgatcagcaa cacgcttagt
     1261 ctccggatcc ggtccaccgc ccgacccagc gcttccaggc tggtggggtc ggaccaatgc
     1321 gcatcctcga agaccatcag caccggagtg gagcgggcca gggcttctac ttgcgagacg
     1381 agtgcttcca gcgttctttg ccggcgctgc tgcgcggtga gctcgaccgc gggatagcgt
     1441 ccatcgttcg gcagcgacaa catctcggca aagagcgcgg catcctgcgc agatgtcgag
     1501 gtctgcttga gcacgcgatc gagcttgtcg agtttcgctt gcgagctgtc gtcgcgcgcc
     1561 agtccggcgg cacgttccat ttggccgatg atcggataaa gcgcactgtc ggtgtgttgc
     1621 ggcgagcaga aatagcgcaa gcgtatgtgc ggctcgccag caagaagtcc cacaaacgcg
     1681 gt
//
LOCUS       DATLJT010001247         7624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3543683,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001247 DATLJT010000000
VERSION     DATLJT010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3543683"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VM910_28655"
     CDS             complement(<1..641)
                     /locus_tag="VM910_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI66518.1"
                     /translation="MSTFEHIIVESRGAVGIITLNRPKMLNALSFGVFREIAAAVDDL
                     EADDAIGCIVITGSEKAFAAGADVKEMQPKGFLDMFADDFATAGAGRVAKCRKPTIAA
                     VAGYALGGGCELAMMCDIIIAADTAKFGQPEITLGTIPGIGGTQRLTHAVGKSKAMDL
                     CLTGRMMDAAEAERSNLVSRVVAADKLMDETLAVAERIASMSQPVAIMAKEAI"
     gene            complement(801..1193)
                     /locus_tag="VM910_28660"
     CDS             complement(801..1193)
                     /locus_tag="VM910_28660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66519.1"
                     /translation="MKNVASRCSLLAIGLLVCVAEPSHAAPARFDTAAMQGLVAQAQR
                     DSLTPVAEKQVVPADDLNEIDRAAQEQTSPAPPVAVPSNEEQKRRTTVAMASSDHSVW
                     DETSLIGKIFIGLGTLLTLASAARMFMA"
     gene            1536..2081
                     /locus_tag="VM910_28665"
     CDS             1536..2081
                     /locus_tag="VM910_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI66520.1"
                     /translation="MREREDEWTGLMRSALSGDGAAYHRLLKSVTPVLRAAARRGLAR
                     AGQPVDQSEDIVQDILLAVHMKRHTWDANAPFAPWLFAIARNKLIDALRRRGKRVFVN
                     IDDFAETLADQPPAETASANVVALQLKSLPPRQRDVLQSIAVESASIKDTARKFAMSE
                     GAVRVALHRALASLTAKLREQ"
     gene            2083..2721
                     /locus_tag="VM910_28670"
     CDS             2083..2721
                     /locus_tag="VM910_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HVI66521.1"
                     /translation="MDTDQLIRTLAADNPHRARPVGFVLALALLAAAPVSLLMFFAEL
                     GVRPDVMTAMHNPFFDLKFAVTLALAAAAITIALHLSRPEASLRGWVWLLIIPAGLLV
                     GGISGEMMVPQRLPMMTRLVGSNSKVCMTAIPMMSLPLLAASLVGLRHGAPTRPAAAG
                     AMAGLLSAGLAATVYASHCTDDSPLFVATWYSLATALVTALGALLGSKLLRF"
     gene            complement(2737..3858)
                     /locus_tag="VM910_28675"
     CDS             complement(2737..3858)
                     /locus_tag="VM910_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HVI66522.1"
                     /translation="MTSRPAAASENLLDELQSALAHGTVSRRVETLRRVTDLFLNGAV
                     DYSDEQIELFDDVFQCLIQHIEVSAKALLANRLAPIDTAPPLTIRTLAFDDVIEVAGP
                     VLSQSNRLDDAALIEAARCKSQAHLMAISKRKALSDAVTDVLVLRGNHEVIQSTVNNP
                     GAEFSERGFTRLVNRAEGDDNLATCLGLRPNMPRHLYLKLLAKASDTVRQRLEAANPG
                     QAGEVPMAVREATRRARSAPSAISKDTAIAHSLVKSLFEDGRLDERQVAQFAAAGKFD
                     ETNAAIAALANVSVAIAENMMLETRAEGVMILAKVSGLSWSTVKSIIDMRDELSGAEP
                     TDLNACRETYERLRPSTAQQVLRFHRMQQAATPTEAPAT"
     gene            complement(3927..4955)
                     /locus_tag="VM910_28680"
     CDS             complement(3927..4955)
                     /locus_tag="VM910_28680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVI66523.1"
                     /translation="MIASSYLLDGVILLLYAEAGTVPLNIGAAYVATGLVSVVLYLAL
                     SETGFTERFKDHYFVVPQLFVGTAIMLAFTYLAPEVGVMFLCTLFLVVNFGSLRSTPA
                     QTAVVWTMMTVGVAALFLLTNKPLGMPHANHLERFATMLVFVLTIGRCMFLGIFSSSM
                     RQSLYKSGLKLKEAYRRIEELAELDELTGSFNRRSIMRMLEEEIARSHRLNAPCTVAL
                     IDLDFFKRINDLYGHPTGDEVLRTFAITAFANIRSVDRFGRYGGEEFLLILPDTDETS
                     AVQMLDRLRGIIAELDWSAFSSGMRVTISAGVAMLRANEISDTLLARADGALYASKAR
                     GRNRITSA"
     gene            5501..6067
                     /locus_tag="VM910_28685"
     CDS             5501..6067
                     /locus_tag="VM910_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI66524.1"
                     /translation="MSATTVTDDVLIARIAQGDRLAMQVLYGRHHVRVFRFGLRLVRD
                     EQVAEDLIGEVFLDVWRQAGKFEGRSAVSTWLLAITRFKALSALRRRKDLELEEEAAN
                     AIEDTSDDPEVAVTKKDTSEALRECLTGLSREHREIVDLVYYHEKSVEEVAEIVGIPE
                     NTVKTRLFYARKKLAELLKAAGVERGWP"
     gene            6064..6762
                     /locus_tag="VM910_28690"
     CDS             6064..6762
                     /locus_tag="VM910_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66525.1"
                     /translation="MMALSKRKMEQQASEIELLLPWHAAGTLNTRDARRVEEALAREP
                     ELARQYAVIREEYTETIALNESLGAPSVRAMQKLFAAIDAEPQVTVRPSLPLSARVSA
                     FVAGLSPRTLAWSASLGAIALVFQAGVIGAVLLKNQSVAYQTASLSMNEPITRALGEQ
                     TPPRALVRFVPEARISDITVLLHAYQATIVGGAKGGMFELQFGNKTMTKEEVADLMGR
                     LQREKIVGLAVATP"
     gene            6797..>7624
                     /locus_tag="VM910_28695"
     CDS             6797..>7624
                     /locus_tag="VM910_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HVI66526.1"
                     /translation="MSDQASLRKAVRPAALALTIAVLPLVGAANLPAQAQSFVRSPNL
                     NIGPRIPTINATPRINPNVATVRTISPNLNVRQSCSYAYRTSDGQCSGQPVSTSGGGG
                     TGNGSAGAGRSGGSRNNVTATNPNQRITNELVAEIDGALTDRQADALARRHRLIRLGS
                     QNFPLIGATIGRFRIADSRSADAASRDFASDASVHAVQPNFRYVLQEQQAIPTEGDPA
                     QYAVAKLRLAEAHTLAHGTNIRVAVIDSGIDASHPELSNSVVESFDALGSSEGAHVHG
                     "
BASE COUNT         1383 a         2361 c         2461 g         1419 t
ORIGIN      
        1 ttgatggctt ccttggccat gatggccacg ggctgcgaca tcgaggcgat cctctccgcc
       61 acggcgagcg tctcgtccat caatttgtcg gccgccacca cgcggctgac gagattgctg
      121 cgctcagcct ccgccgcatc catcatccgc cctgtgaggc agaggtccat tgccttggac
      181 ttgccgaccg cgtgcgtgag ccgctgggtc ccgccgatgc cggggatggt gccgagcgtg
      241 atttccggtt ggccgaattt cgcggtgtcg gcggcgatga tgatgtcgca catcatcgcc
      301 agttcgcagc cgccgcccag cgcgtagccg gcgacggccg caatggtcgg tttacggcat
      361 ttcgccaccc ggccggcgcc ggccgtggca aaatcgtcgg caaacatgtc caggaagccc
      421 ttcggctgca tttccttgac gtcggcgccg gcggcaaaag ccttttcgct gccggtgatc
      481 acgatgcatc cgatcgcatc gtcggcctcg aggtcatcga cggcggccgc gatctcgcga
      541 aacaccccga acgagagggc gttgagcatc ttgggccggt tcagcgtgat gatgccgact
      601 gcgcccctgc tctcaacgat gatgtgctcg aacgtgctca tgatggatcc cgcaaagctg
      661 aaaatttcgg cgcaatttgc cccctggcgg gatcggcttc aagtggggcg gatggtccga
      721 ttcagaccat tcgcgggcca tcggagcaag ccacgtttgc gaatgtcgga tcagggcccg
      781 gccagcccga cgcgccggcc ttacgccatg aacatgcgcg cagcggaggc gagcgtcaaa
      841 agcgtaccga ggccgatgaa gatcttcccg atcagcgagg tttcgtccca gaccgaatga
      901 tcgctgctcg ccatcgcgac ggtcgtgcgc cgcttctgtt cttcgttcga ggggactgcc
      961 acgggtggcg cgggggatgt ttgctcctgt gcagcccgat cgatttcgtt gaggtcgtcg
     1021 gcgggaacca cctgcttctc tgctacgggc gtcagactgt cccgttgggc ctgcgcaacc
     1081 aacccctgca tggcggccgt atcaaacctg gcgggcgccg cgtgcgaagg ctccgcaaca
     1141 cacaccagga gcccgattgc gaggagtgaa caccggctgg ctacgttttt cattcttgat
     1201 tgtctcctca ttcgccagtc ccaggcgcgg aatgagactt gaaatgaggt ttgggccgtg
     1261 tcggcagcgc ggcagaaaaa gggaatcttc gcgctggcgc cggcaaaagc tgggcagcgt
     1321 ccatcggtgc tttgtttgcc gttgtcgtgc gcgaaattct cgcgcaatcg cgcaaacggt
     1381 cctatgcaac gcgacgccgc gcatgaccgt cggaccgcgc gcagctatga ccacttgcaa
     1441 agctgtgacc tcataagcgc gatcacagct tcgtgattgg cgctttccgt cgtaacaatt
     1501 tgaaacgtcg aacgaattgc atgatgggag cgcgcgtgcg cgagcgtgaa gacgaatgga
     1561 ccggcctgat gcggtcggcc ctgtccggag acggcgcggc gtatcaccgg ttgctcaaat
     1621 cggtcacgcc tgtgttgcgg gctgcggccc gccgcgggct tgcgcgggcg gggcagccgg
     1681 tcgatcaatc cgaggacatc gtgcaagaca ttttgttggc ggtgcatatg aagcggcata
     1741 cctgggatgc gaacgcgccg tttgcgccct ggctgtttgc gatcgcccgc aacaagctga
     1801 tcgacgcgct gcgccgccgc ggcaagcgcg tgttcgtcaa tatcgacgat tttgccgaga
     1861 ccctggccga ccagcctcct gccgagaccg cgtccgccaa cgtggttgcc ttgcagctca
     1921 aatcgctgcc gccgcgtcag cgcgacgtat tgcagtcgat cgcagtcgag agcgcctcga
     1981 tcaaggacac ggcacgaaaa tttgccatga gcgaaggcgc ggtgcgcgtg gcgctgcacc
     2041 gcgcgcttgc gagtctcacc gcgaagctaa gggaacaatg agatggatac cgatcagctc
     2101 attcggaccc ttgctgcgga caacccgcac cgtgcccggc ccgtcggctt cgttctggca
     2161 ttggcgctgc tcgcggccgc gccggtttcg cttctgatgt tctttgccga actgggcgtc
     2221 aggcccgacg tgatgacggc gatgcacaat ccgttcttcg acctgaaatt cgcggtgacg
     2281 ctcgcgcttg ccgcggcggc gattacgatc gcgctgcatc tgtcgcggcc ggaggcgtcg
     2341 ttgcgcggct gggtgtggct gttgatcatt ccggcagggc ttttggtcgg cggcatctcc
     2401 ggcgagatga tggtgccgca acgcctgccg atgatgacgc gcctggtcgg cagcaattcg
     2461 aaggtctgca tgacggcgat cccgatgatg tcgctgccgc tgctggcggc ctcgctcgtc
     2521 ggcctgcggc acggcgcacc cacgcgcccg gcggccgcag gcgcgatggc cggattattg
     2581 tcggccggac tggcggcgac cgtctacgcc tcgcattgca ccgacgactc gcccttgttc
     2641 gtggcgacct ggtacagcct tgcgactgcg ctcgtcaccg cgcttggcgc actgctcgga
     2701 tcgaaactgc tgaggttcta gagcgcgatc gttgcgctaa gtcgccggcg cctcggtcgg
     2761 cgttgccgcc tgctgcatgc ggtggaagcg cagcacctgc tgggcggttg acggccgcag
     2821 ccgctcgtag gtctcccggc aggcattcag gtcggtcggc tcggcgcccg agagctcgtc
     2881 gcgcatgtcg atgatcgact tcaccgtcga ccacgacaag ccggatacct tggcgaggat
     2941 catcacgccc tccgcgcggg tctccagcat catgttctcg gcgattgcga cggatacatt
     3001 ggccagcgcc gcaatcgccg cgttggtttc gtcgaacttg ccggctgccg cgaattgcgc
     3061 gacctggcgc tcgtcgaggc ggccgtcctc gaagagcgac ttcaccagcg aatgcgcgat
     3121 cgccgtgtcc ttcgatattg ccgacggcgc cgaacgcgcg cgccgcgtcg cctcgcgcac
     3181 cgccatcggc acctcgccgg cctgtccagg gttggcggcc tcgagccgct gccgcaccgt
     3241 atcggacgcc ttcgccagga gtttcaaata gagatgccgc ggcatgttgg gcctgagccc
     3301 gaggcaggtc gcgaggttgt cgtcgccctc tgcgcggttg accagacggg tgaatccgcg
     3361 ctcggaaaat tcggcgccgg gattgttgac cgtcgactgg atcacctcgt gattgccgcg
     3421 caacaccagc acgtcggtca ccgcatcgct caaggccttg cgcttcgaga tcgccatcaa
     3481 atgcgcctga ctcttgcagc gtgccgcctc gatcagcgcc gcgtcgtcga gccggttgga
     3541 ttgcgacagc acggggcctg cgacctcgat gacgtcgtcg aatgccagcg tgcggatcgt
     3601 gagcggcggc gcggtgtcga tcggcgccag gcggttggca agcagcgctt tcgccgagac
     3661 ctcgatatgc tggatcaggc actggaagac atcgtcgaac agttcgatct gctcgtcgga
     3721 atagtccacg gcgccgttga ggaacaggtc cgtgacgcgg cgcagtgtct cgacgcgtcg
     3781 ggacaccgtg ccgtgcgcga gggctgactg cagctcatcg agcaggtttt cggaggcagc
     3841 ggcaggtctg gaagtcatct tgtctctgtc ctggagcttg aggcggcaac gatttcttcg
     3901 tcgccaaaaa tggatgggac ttcgtttcag gcactcgtga tgcgatttcg tcctcgggcc
     3961 ttcgaagcgt aaagcgcgcc gtcggcgcgg gcgagtagcg tgtcggaaat ctcgtttgcg
     4021 cggagcatcg caacgcccgc ggaaatcgtc acccgcatgc cggacgaaaa tgcgctccag
     4081 tcgagttcgg cgatgatgcc gcgcagccgg tcgagcattt gcacggcgct cgtctcgtcc
     4141 gtgtcgggaa gaatcagcag gaattcctcg cccccgtagc ggccgaagcg gtcgaccgag
     4201 cggatattgg caaaggcggt gatggcgaag gtgcgcagca cttcgtcgcc ggtcggatga
     4261 ccatagaggt cgttgatgcg cttgaaaaag tcgaggtcga tcagcgccac cgtgcaaggc
     4321 gcgttgaggc gatgcgagcg ggcgatctcc tcttccagca tccgcatgat gctgcgccga
     4381 ttgaaggagc cggtcagttc gtcgagctcc gcaagctcct cgatgcgccg ataggcttcc
     4441 ttcagcttca gcccgctctt atagagcgat tgccgcatcg agctggaaaa gatcccgaga
     4501 aacatgcagc gcccgatcgt cagcacgaac accagcatgg tggcaaagcg ttcgagatga
     4561 ttggcatgcg gcattccgag cggcttgttg gtcaaaagaa acagcgcagc cacgcctacg
     4621 gtcatcatcg tccacacgac cgccgtttgt gccggcgtcg agcgcagcga gccgaaattg
     4681 acgacgagaa acagcgtgca gagaaacatc acgcccactt cgggcgcgag ataggtgaag
     4741 gcaagcatga ttgccgtgcc gacgaagagc tgcggcacga cgaaataatg gtccttgaag
     4801 cgctcggtaa atccggtttc agacagcgcc aggtaaagca cgaccgagac gagacccgtc
     4861 gccacataag ccgcaccgat gttcaaaggc acggtgccgg cctcggcata gagcagcagg
     4921 atcacgccat cgagcagata gctcgacgcg atcattccct gaatctgccg ccgttgccgc
     4981 gcccggcgcg atcgcacttt tgccgtcagc ttgagaccaa cgccatccag ctcagccgca
     5041 atcggattca gaaaaggcgt cgcagtgctg cccatcgtct ggccattggt gaaaccggtt
     5101 ccaaaatcta agtgggaaag cctttaggtt tggtatcttt cgtaccaaat cccgccgccg
     5161 gccttcgcag cgacggccat tgatcggcaa tggaaatggg ctgccacgaa gcacaaaagc
     5221 ccgctcgccg cgcatcctgt tgagcgtgac acgcaaaggc tgttctgcga cgggagcctt
     5281 gctaaggtcg aacagggtgg ggcgaagaat cgggcgtgct tctctactat gatacctgct
     5341 ttgggggttg tccggcgccg tgccatcagg gacgggcgga cccatttcct ttgtgcggta
     5401 ggtcacagaa gttcgggaac tattgcgata atttgaagaa tcttgctcat ggcatcgaac
     5461 cagccgccaa ttcattccga ccaaccctgc gagacggcca ttgagcgcaa cgacggttac
     5521 ggacgatgtc ctgatcgcca ggatcgctca aggcgaccgg cttgccatgc aggtgctgta
     5581 cgggcggcac catgtcaggg tgtttcgctt cgggcttcgg ctcgtgcgcg atgaacaagt
     5641 cgcggaagac ctcatcggcg aggtttttct cgacgtatgg cgtcaggcgg gcaagttcga
     5701 aggccgttcc gccgtctcca cctggctctt ggcgattacg cgattcaagg cgctctctgc
     5761 gctcaggcgc aggaaggacc ttgaactgga ggaggaggcc gccaacgcga tcgaggatac
     5821 gtccgacgat ccggaagttg cggtcacgaa gaaggatacg agtgaagcgt tgcgcgagtg
     5881 cctgacgggg ctttcgcggg agcatcggga gatcgtcgac ctcgtctact accacgagaa
     5941 gtcggtggaa gaagtggccg agatcgtcgg catcccggag aacacagtga agacgcgcct
     6001 gttctatgcg cgcaagaaat tggccgagct gctgaaagca gccggcgtgg aacgaggctg
     6061 gccctgatgg cattgagcaa aagaaaaatg gaacaacagg ccagtgagat cgagctgctg
     6121 ctgccctggc atgcggccgg cacgctgaac acgcgcgatg cgcggcgcgt cgaggaagcg
     6181 cttgcccgag agcccgagct cgcccggcag tatgcggtga tccgggaaga atataccgag
     6241 accattgcgc tcaacgaaag cctcggcgct ccatcggttc gcgcgatgca gaaattgttt
     6301 gcggcaatcg acgccgagcc gcaggtcacg gtacggccct cgctgccgct ttcggcacgc
     6361 gtttccgcct tcgttgccgg cctgtcgccg cggaccctgg cctggtcggc aagtctcggc
     6421 gcgatcgcgc tggtgttcca ggccggcgtc atcggcgcgg ttctgctgaa gaaccagagc
     6481 gtagcctacc agaccgcgtc gctcagcatg aacgagccga tcacgcgcgc gctgggcgaa
     6541 cagacgccgc cgcgcgcatt ggtgcggttc gttccggagg cgcggatttc cgatatcacg
     6601 gtcctgctgc acgcctatca ggctaccatc gtcggtggcg ccaagggcgg catgttcgag
     6661 ctgcaattcg ggaacaagac catgacgaag gaagaggtcg cagacctcat gggcaggctg
     6721 caacgcgaga agatcgtcgg tcttgcggtc gccacccctt aaggcgccac tgccgctcaa
     6781 gtccggggtt ctggcgatgt ccgatcaagc gagcttgcgg aaagcggtgc ggcccgcagc
     6841 gctggcgctc accatcgcgg tgctgccgct cgtcggtgct gcgaacttgc cggcgcaggc
     6901 gcaaagtttt gtgcgctcgc ccaatctcaa cattgggccg cgaatcccca cgatcaacgc
     6961 gacaccgcgc atcaatccga acgtcgccac cgtcaggacg atctcgccaa acctgaatgt
     7021 gcggcaatcc tgcagctatg cctatcgcac gagcgatggc caatgcagcg gccagccggt
     7081 ttcgacgagt ggtggcggcg gtaccggcaa cgggtcggcg ggcgccggca ggtcgggcgg
     7141 ttcgcgcaac aatgtcacgg cgacgaaccc gaaccagcgg atcaccaacg aactcgtcgc
     7201 cgaaatcgac ggcgcgctga ccgaccggca ggctgacgcc ctggcgcggc ggcaccgtct
     7261 gatacggctc ggttcgcaga attttccact gatcggtgcg accatcggac gtttccgcat
     7321 cgccgacagc cgctcggcgg atgcggccag ccgcgatttc gcaagcgacg ccagcgtgca
     7381 tgcggtgcag ccgaatttcc gctatgtgtt gcaggagcag caggcgattc cgaccgaggg
     7441 cgatccggcg caatatgctg tcgccaaact gcgccttgcc gaggcgcata cgctcgccca
     7501 tggcaccaat atccgggtcg ccgtcattga ttccggaatc gacgcgagcc atccggagct
     7561 ttccaattcg gtcgtcgaaa gttttgacgc gctcggcagc agcgaggggg cgcatgtcca
     7621 tggc
//
LOCUS       DATLJT010001248         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_784346,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001248 DATLJT010000000
VERSION     DATLJT010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784346"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VM910_28700"
     CDS             complement(<1..254)
                     /locus_tag="VM910_28700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66527.1"
                     /translation="MIRDTLIRDDANKQISFDDRSMQFGRSVATSRHDFTTAFELAPD
                     AQRLFPSDMERQHLKLMDMIAAIVGALDERELFQSIIGDTG"
     gene            complement(273..1030)
                     /locus_tag="VM910_28705"
     CDS             complement(join(273..691,691..1030))
                     /locus_tag="VM910_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612353.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVI66528.1"
                     /translation="MRRNLFWLSDERWKRIEPYLPTDVRGVERTDDRRVISGIVHVLK
                     SGCRWCDCPPEYGPPTTIYNRFVRWARRGIWENLFRELARNGRSTDTQMIDSTHVKAY
                     RSAAGGKGGRRKQAIGRSRGGRNTKIHALADVKGRLIAILLTGGEAHDCPVADRLVRR
                     MRPSKRILGDTAYDSAELREDLQERGTKPVIPNRCNRKQPFSFSKRVYKLRWRIETAF
                     NRLKDFRRIATRYDRLARNYLASVCLAAALVWWI"
     gene            complement(1293..2867)
                     /locus_tag="VM910_28710"
     CDS             complement(1293..2867)
                     /locus_tag="VM910_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HVI66529.1"
                     /translation="MARRDALEAAASCSYPWDAPGLFRAGRVISFLEDLEISTGKHAR
                     TKLRLRPWQRKFVETVYRENKRGIRPIRTAVLSMARKNGKSQLAAALALCHLCGPEAE
                     SRGEIYSCANDRFQSARIFNEMVALIDRHPALSARTNVTSFRKEIYDLVNGSIYVALT
                     AEAKTKLGLSPSFVIYDELGSADGPALFDAMDSALGARENPLMLVISTQAADDFAPMS
                     QLIDYGLKLKGGEIHDPAFHLTLYTAPENADPWSRKAWRAANPALGDFRSLPDVERLA
                     KQAQRMPARENSFRNLILNQRVAAEARFIDPTAWRACSGEPNIPVGVRVYAGLDLGAT
                     RDLSALAVVYQDAAGVFHTKLHCWLPGNLQERSEQDHAPYSAWQKLGFITPIGPTTDP
                     RAVAYKIAELNGQNRIMSLAFDRWRINDLKRELDAISCSVPLEPHGQGYKDMSPAVDI
                     VERLVVQKKLRHGANPVLSWCAANAVVTRDPAGGRKFDKAKSTGRIDALVALAMALSL
                     ALLRATKPIDIEALIG"
BASE COUNT          651 a          964 c          948 g          668 t
ORIGIN      
        1 cccgtatcac cgatgatcga ctgaaacagt tcgcgttcgt ctagtgcgcc cacgattgcc
       61 gcgatcatgt ccatgagctt taggtgctgt ctttccatat cgctggggaa caggcgctgg
      121 gcgtcgggag cgagttcaaa agcggtggta aaatcgtgac gcgatgttgc gacggaccgg
      181 ccaaattgca tcgatcgatc gtcgaatgag atctgcttgt ttgcgtcatc gcggattagc
      241 gtgtcccgga tcacgactag ggtccagact cattaaatcc accatacaag agcagcggcg
      301 aggcagacag aggccaggta atttcgtgcc agcctatcgt agcgggttgc gatgcgcctg
      361 aagtccttta atctgttaaa cgcggtctcg atgcgccagc gcagcttgta aacacgcttg
      421 ctgaagctga acggttgttt cctgttgcag cgatttggaa tgaccggctt ggttccgcgc
      481 tcctgcaagt cttcgcgcag ctcggcgcta tcgtaggctg tgtcgccgag tatacgtttc
      541 gacggcctca ttctgcgaac gaggcggtca gcgaccgggc aatcgtgtgc ttcacctccg
      601 gtcaacaaga tggcgataag gcgcccttta acatctgcga gtgcgtgaat cttcgtgttg
      661 cgccctccgc gcgagcggcc aatagcctgc ttctcctccc cccttttccg cctgctgccg
      721 agcggtacgc ttttacgtgt gtggaatcga tcatctgcgt gtctgtggat cgtccgttcc
      781 tagcaagctc cctgaacagg ttttcccaga tgccgcgccg cgcccagcgc acaaagcggt
      841 tatagatcgt ggtcggagga ccatactccg gcgggcagtc gcaccagcga cagccgctct
      901 tcagtacatg cacgatgccg ctaatgaccc ggcgatcgtc cgtccgctcg acgcccctga
      961 catccgtcgg cagataaggt tcgatccgct tccatcgctc atcgctcagc caaaacaggt
     1021 tcctgcgcac gatcccaatc cccttccaaa atcgaatcag gatcgtattg tcagagcgga
     1081 gaaattaatg agttctgacc ctagtttgtg gagcgccgtc acgcagccgc cgcaagcacc
     1141 ggatcggcaa gatggggagc gccgtatttc cggccttcac tcccgcggca ttcgcagaaa
     1201 ccgcagatga tcaggtgcgc gtcgaggaac cccggcagta ccgaaccgct gcaagtaaat
     1261 gtgaaaagcc gccaactcgg gcacttacaa gctcaaccaa tgagcgcctc gatatcgatc
     1321 ggcttcgtgg ctctcagcaa agctaacgac aacgccatgg caagcgcgac caacgcgtca
     1381 attcgacccg tactcttggc tttgtcaaac ttgcgtccgc cagcagggtc gcgggtcacg
     1441 acggcattgg cggcgcacca actcaggacg gggtttgccc cgtggcgcag cttcttctgg
     1501 acaaccaggc gctcgacgat atcgaccgct gggctcatat ctttataacc ttggccatgc
     1561 ggctcgagcg ggacgctgca gctgatggcg tcaagctcgc gcttcagatc gttgatgcgc
     1621 cagcggtcaa aggcgagcga cataatgcgg ttctgcccgt tcaactcggc gatcttgtaa
     1681 gcgacggcgc gcgggtcggt tgttggccca atcggcgtga tgaaaccgag cttctgccac
     1741 gcgctgtaag gggcgtggtc ctgctcgcta cgttcctgca gattgcccgg aagccagcag
     1801 tggagtttcg tgtgaaaaac gccggcagcg tcctggtaga cgaccgccaa ggcgctcaga
     1861 tcacgggtgg cgccgagatc cagtccagca taaacgcgaa cgccgacggg aatattgggc
     1921 tcgccggagc aagcgcgcca cgcggtaggg tcgatgaatc gcgcctcggc agcaacgcgc
     1981 tggttcaaaa tcaaattcct gaacgagttt tcgcgtgccg gcattcgttg cgcctgtttt
     2041 gcaagccgtt cgacgtcagg gagcgaacgg aagtcaccga gcgccggatt ggcggcacgc
     2101 caagcctttc ggctccacgg atccgcgttc tcgggcgcgg tgtagagcgt cagatgaaag
     2161 gcagggtcgt ggatctcacc acccttgagc ttgaggccat aatcgatgag ttgggacatg
     2221 ggcgcgaaat cgtcggccgc ttgggttgaa atcaccagca tcagggggtt ctctcgtgca
     2281 cccaaggcgc tatccatcgc gtcgaaaagc gcagggccgt cggcgctgcc aagctcgtcg
     2341 tagatgacga acgacgggct taaccctagt ttcgtcttcg cctcggcggt caaagcaacg
     2401 tagatggagc cattgaccag atcgtagatc tccttccgga acgacgtgac gttggtacgc
     2461 gcggacaggg ccgggtgacg atcaattaac gcgaccattt cattgaaaat ccgtgccgac
     2521 tgaaaacgat cgttggcgca ggagtagatt tcgccgcgac tctccgcctc cgggccgcag
     2581 agatggcaga gcgctaaggc ggcggcgagt tgcgatttgc cgttcttcct cgccatgctc
     2641 aaaacagccg tgcgaatcgg cctaatgccg cgcttgttct cgcgatagac agtctcgacg
     2701 aacttgcgtt gccacgggcg cagacggagc ttcgtgcgcg catgcttgcc ggtgctgatc
     2761 tcaaggtctt cgaggaagct aatcactcgc ccggcgcgaa agagcccggg ggcgtcccag
     2821 gggtatgagc acgaagctgc cgcctcgagc gcgtcccgcc gcgccatcag aggttttgca
     2881 cctggtcctc ggaggcccat atctcatgac caattggggt ggcgccataa ctggagccta
     2941 ccacatattg aggcgtccgt gattaatgcc actaaacctg cgataaaccg aggctctttg
     3001 ccgccgaccc gagccccaac tatgcctgtc cgggagagaa gtttggtgtt ccataaccca
     3061 ttgatattat gcataaaact aactctgcga gcgatcgcgc gagcgccgcg agctttggag
     3121 tcttgatttt tttggacctc cccccggccc cgcaagcagc ggctgtccgg ccgatggtgg
     3181 atcatggcgc cctacgatca cccacgaacg agcaccctac tgtttgccgc t
//
LOCUS       DATLJT010001249         5017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_96382,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001249 DATLJT010000000
VERSION     DATLJT010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96382"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="VM910_28715"
     CDS             complement(<1..2011)
                     /locus_tag="VM910_28715"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVI66530.1"
                     /translation="MQSTLDFSHYLKNAGPGLLHLELAVEGINCAGCMAKIERNLSKI
                     PDVTSARVNLTDRRLALEWKEGAIDPATFVDRLAELGYKAYPFERADAETKEAERAGA
                     LLRRLGVAAFAAMNVMMLSIPVWSGNVSDMLPEQRDFFHWLSALIVLPAAAYSAQPFF
                     SSAFAALRARGVNMDVPISIGIALALGMSLVETASHADHAYFDAAIMLIAFLLAGRYL
                     DQNMRRRTRAFAGNLAALKAETATKFFGDDEVRTVPVAAIRPGDIVLLRPGERSAVDG
                     NVVSGRSEIDQSLITGETKPETAVAGSAVYAGTLVRSGTLRVRVAAAAEETLLAEISR
                     LLENALQSRSHYLRLAERAARLYAPVVHATAFLTMLGWLIAGATIHDSIVTAIAVLII
                     TCPCALGLAIPAVQTVASGALFGSGVLLNSGDAIERLALVDRVIFDKTGTLTLPELDV
                     ANAASIPAAVFALAGRVALSSRHPVAAAVARAAKATSPLEGIEEEPGQGVHGMLDGRE
                     IRLGRPVYCGADDEANAILSRDPEASVVAFRHGADCHVFAVRQRLRPDAASVVEKLRQ
                     YGIAVEVVSGDREPAVRMAAQSLGIREWRAEVTPVDKIARINELAAQGFKVLMVGDGL
                     NDAPALAAAYASMSPVSATHLSQSVADAVFLGERLAPVGLAIAISR"
     gene            complement(2022..2519)
                     /locus_tag="VM910_28720"
     CDS             complement(2022..2519)
                     /locus_tag="VM910_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HVI66531.1"
                     /translation="MSTRISERRLTGRKVLAMLIAFFGIVIGVNVTMAKLAIKTLPGT
                     DVDSAYTASLGYEKEILAARDQSARNWKVDAHIERLAGGAALLQVEARDRDGRPVTGL
                     KFQGRFERPTDKRADLAIALAEVGIGIYRGRLDAVAPGQWDLVLEGDNASGRMFLSKN
                     RVLLN"
     gene            complement(2541..3992)
                     /gene="ccoG"
                     /locus_tag="VM910_28725"
     CDS             complement(2541..3992)
                     /gene="ccoG"
                     /locus_tag="VM910_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HVI66532.1"
                     /translation="MTSQDLQYEDYGPLYASRKKVYPQSVSGTFRRIKWGLMAVCLSI
                     YYFLPFVRWNRGLGAPDQAVLIDFPNRRFYFFFIELWPQEVYYFTGLLIIAAVALFLM
                     NSIGGRIWCGYLCPQTVWTDLFYAVERLIEGDRRERMKKDAAARRWTPRRIYEMVAKH
                     TIWLLIAWWTGGAWVLYFSDAPTLVKDLATLQAPMVAYAWIGILTATTFVLAGFMREQ
                     VCTYMCPWPRIQASLTDEWALNVTYRYDRGEKRTSVKKAVELRALGEHVGDCIDCYQC
                     VAVCPTGIDIRNGPQLECIQCGLCIDACDNVMTKIGRPKRLIAYDNDINIQRRQKGQA
                     PIYRIVRPRTIIYALVIALVGGIMLYTLATRSLLDANVLHDRNPVAVRLSDGSIRNAY
                     TMRLLNKSGFDRVVTVDIDGPANAIVHVVGADSVTPDRPMIVIGRDQTTELRLLVTAP
                     EENNREKSIPVHFHVTDIGLGVVASATDNFVAP"
     gene            complement(4093..4965)
                     /gene="ccoP"
                     /locus_tag="VM910_28730"
     CDS             complement(4093..4965)
                     /gene="ccoP"
                     /locus_tag="VM910_28730"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HVI66533.1"
                     /translation="MTDHGDIDSVSGTATTGHQWDGIKELNTPLPKWWLYTFYLTIIW
                     AVGYWIVYPAWPLLRSNTPGILHYSSRANVAVELANLDKLRGEKMAALSAASLADIEK
                     DPALLALARARGKTVFGDNCAPCHGSGAAGAKGYPNLNDDDWLWGGTLDQIMQTIRYG
                     ARAGNAKTHEGQMLAFGKEGVLKPDQIVTVANYVRSLSGLPTRPGYDAAAGAKIFAEN
                     CTACHGENGKGNQELGAPDLTDKIWLYGSDEATVIETITNGRSGVMPAWEGRLDPVTL
                     KAMAVYVHSLGGGK"
BASE COUNT          928 a         1606 c         1618 g          865 t
ORIGIN      
        1 tgcgcgagat ggcgatcgca agacccaccg gcgcgagccg ctcgccgaga aacacggcat
       61 ccgccacgga ctggctcaaa tgggttgccg aaaccggcga catcgaggca taggctgcgg
      121 caagcgcggg cgcatcgttc agcccgtcgc cgaccatcaa gaccttgaaa ccctgagccg
      181 cgagttcgtt gatccgcgcg atcttgtcga ctggcgtcac ctcggcgcgc cactcgcgaa
      241 tcccgagcga ttgcgctgcc atccgcaccg ccggctcgcg atcgcccgag accacctcga
      301 cggcgatacc gtactgtcga agcttttcga cgacgctagc tgcgtccgga cgcaggcgtt
      361 gcctcacagc aaaaacgtga cagtccgcgc cgtgccggaa ggcgaccacc gacgcctcgg
      421 ggtcccggct caaaatcgca ttcgcctcat cgtcggcgcc gcaataaacg gggcggccca
      481 gcctgatctc gcgaccgtcg agcatgccgt ggacgccctg ccccggctcc tcctcgatgc
      541 cttcgagcgg cgacgtggct ttggcggcgc gcgccacggc ggccgcaacg ggatgacggc
      601 tggagagcgc cacgcgcccg gcgagtgcga acacggcggc gggaatggag gcggcattgg
      661 cgacgtcgag ctccggcaac gtcaatgtcc cggtcttgtc gaagatcacg cgatcgacca
      721 gcgccaggcg ctcgatcgca tcacccgaat tgagcagcac gcccgagccg aacagcgcgc
      781 ccgaggcgac tgtctgcacc gcagggatgg caaggccgag cgcgcaggga caggtgatga
      841 tgagaacggc gatcgcggtc acgatcgaat catggatggt tgcccctgca atcagccagc
      901 ccagcatggt cagaaacgcg gtcgcatgca cgaccggcgc gtagagccgt gcggcgcgct
      961 cggcaaggcg caggtagtgc gagcgggact gcaatgcatt ttccagaagg cgcgagatct
     1021 ccgccagcag cgtttcctcc gccgctgcgg caacgcggac gcgcagggtg ccggatcgga
     1081 ccagcgtccc tgcgtagact gcgcttccgg ccaccgcggt ttcgggcttc gtctcgccgg
     1141 tgatcaggct ctggtcgatt tcggagcggc cgctgacaac gttgccgtcg acggcggaac
     1201 gctcgccggg cctgagtaac acgatatcgc cgggcctgat cgcggcgacg ggaacggtgc
     1261 gaacctcgtc gtcgccgaag aatttcgtcg ccgtttcggc cttcagtgct gcgaggttgc
     1321 cggcaaaggc gcgggtacgc cggcgcatgt tctgatcgag atagcggccc gccagaagaa
     1381 aggcgatcag catgatcgcg gcatcgaaat aggcgtgatc tgcgtgcgag gcggtctcga
     1441 ccagcgacat gccaagcgcg agcgcgatgc cgatcgagat cggcacatcc atgttgacgc
     1501 cgcgtgcgcg aagtgcggca aaggcggacg agaaaaacgg ctgtgccgaa taggccgcgg
     1561 ccggcagcac gatcagggcg gacagccaat gaaagaagtc gcgctgctcg ggcagcatgt
     1621 cgctgacatt gcccgaccag accgggatcg acagcatcat cacgttcatg gcggcgaagg
     1681 ctgcgacccc caggcgccgc aacagcgcgc cggcgcgttc ggcttccttc gtctccgcat
     1741 ccgcccgctc gaacggatag gccttgtatc cgagctcggc gaggcgatcg acgaacgtgg
     1801 ccggatcgat cgcaccttcc ttccactcga gcgcaagcct tcgatcggtg agattgactc
     1861 gcgccgacgt cacgtccggg attttcgaga ggttgcgctc gatcttcgcc atgcagccgg
     1921 cgcaattgat gccttcgacc gctagctcca gatgcaacag gcccgggccg gcgttcttga
     1981 ggtaatgcga aaaatcgagg gtcgattgca tatcgccggc ctcagttcag cagcacgcga
     2041 tttttggaca ggaacatgcg cccggacgcg ttgtcgccct ccagcacgag atcccattgt
     2101 cccggcgcca ccgcatcgag ccggccgcgg tagatgccga tcccgacctc ggccagcgca
     2161 atcgcaagat cggcccgctt gtcggtcggt cgttcgaagc ggccctggaa tttcaatccg
     2221 gtgaccggac gaccgtccct gtcgcgcgcc tcgacctgca agagcgccgc accgccggcc
     2281 aagcgctcga tgtgggcatc gaccttccaa tttcgcgccg actgatcgcg cgccgccaga
     2341 atctccttct cgtagccgag gctcgcggta taggcgctgt ccacatcggt gccgggaagc
     2401 gttttgatgg cgagctttgc catggtgacg ttgacgccga tcacgatgcc gaaaaacgca
     2461 atcagcatcg cgagcacctt gcgcccggtg agccgtctct ccgaaatgcg cgtcgacatg
     2521 atggaagtct cccgcggagt tcagggcgcg acgaaattgt cggtggcgga tgcgaccacg
     2581 ccaagcccga tatcggtgac gtggaaatgg accggaatgg atttctcccg attattctct
     2641 tcgggcgcgg tgaccaggag gcgcaattcg gtggtctggt cgcgcccgat cacgatcatc
     2701 ggccggtccg gcgtcaccga atcggcgccg acgacatgga cgatggcatt ggccgggccg
     2761 tcgatgtcga cggtaaccac gcgatcgaag ccgctcttgt tgagcaggcg catggtgtag
     2821 gcgttgcgga tcgaaccgtc gctcagcctg accgcaaccg gattgcggtc gtgcagcacg
     2881 ttggcgtcga gcagcgagcg cgttgccagc gtatagagca tgatgccgcc cacgagcgcg
     2941 atgacgaggg cgtaaatgat cgtgcgcggc cgcacgatcc gatagatcgg cgcctggccc
     3001 ttctgtcgcc gctggatgtt gatgtcgtta tcgtaggcga tcagccgctt cggccggccg
     3061 atcttggtca tcacgttatc gcaggcgtcg atacagaggc cgcactggat gcattcgagc
     3121 tgcggaccgt tgcggatgtc gatgccggtc gggcagaccg ccacgcactg gtagcagtcg
     3181 atgcaatcgc cgacatgctc gccgagcgcg cgcaactcga ccgccttttt cacggacgta
     3241 cgcttctcgc cgcgatcgta acggtaggtg acgttgagcg cccattcgtc ggtcagcgaa
     3301 gcctggatgc gcggccatgg gcacatatag gtgcagacct gctcgcgcat gaagccggcg
     3361 agaacgaagg tcgtcgcggt caggatgccg atccaggcat aggcaaccat cggcgcctgc
     3421 agcgtggcga ggtctttcac gagggtcggc gcatcgctga aatacagcac ccaggcgcca
     3481 ccggtccacc aggcgatcag gagccagatc gtgtgcttgg cgaccatctc gtagatacgc
     3541 ctgggggtcc accggcgcgc tgccgcgtcc ttcttcatgc gctcgcggcg gtcgccctcg
     3601 atcagccgct cgaccgcgta gaacagatcg gtccacactg tctgcgggca gagatagccg
     3661 caccagatac ggccgccaat cgagttcatg aggaacaggg ccacggcggc gataatgagc
     3721 aggccggtga agtagtagac ctcttgcggc cacagctcga tgaagaagaa gtaaaagcgg
     3781 cggttcggga aatcgatcag caccgcctgg tcgggcgcgc cgaggccgcg attccagcgt
     3841 acgaagggca ggaagtaata gatgctgagg cagaccgcca tcaggcccca tttgatgcgc
     3901 cggaaggtac cggagacgct ctgcgggtaa actttcttgc gggacgcgta gagcggcccg
     3961 taatcctcgt attgcaggtc ctgagacgtc atcgatcgat cctagcctgc catcttcccg
     4021 actttacaac tgaccgcggc ccgggggcag gcttgatcct ggcaggctgg atcaagcttt
     4081 caccggagtt ccctatttcc cgccgccaag cgagtggacg taaaccgcca ttgccttcaa
     4141 cgtcacggga tcgagccgtc cctcccatgc gggcatcacg ccggaacggc cgttggtaat
     4201 ggtctcgatg acggtcgcct cgtcggaacc gtagagccag atcttgtccg tgagatcggg
     4261 cgcgccaagc tcctggttgc ccttgccgtt ctcgccatga caggccgtgc aattctccgc
     4321 aaagatcttg gcgccggcgg ctgcatcata gccgggacgc gtcggcaaac ccgacagcga
     4381 gcggacgtag ttcgcgaccg tgacgatctg gtccggcttc aggacgcctt ccttgccgaa
     4441 ggcgagcatc tgtccctcat gggttttcgc attccccgct cgcgcgccgt agcgaatggt
     4501 ctgcatgatc tggtcgagcg tgccgcccca gagccaatcg tcgtcgttca aattggggta
     4561 gcccttggcg ccggcagcgc cggagccgtg gcagggagcg caattgtcgc cgaacaccgt
     4621 cttgccgcgg gcgcgcgcaa gcgcaagcag cgccggatcc ttctcgatgt cggcaagcga
     4681 agccgcgctc agcgcagcca tcttctcgcc ccgcagcttg tcgagattgg caagttcgac
     4741 cgcgacgttg gcgcgcgagg agtaatgcag gatgcccggc gtgttgctcc tgagcaacgg
     4801 ccaggccggg tacacgatcc agtagccgac cgcccagatg atggtgagat aaaaggtgta
     4861 gagccaccac ttcggtagcg gcgtattcaa ctccttgatg ccgtcccact gatgtccggt
     4921 cgtcgctgtg ccggagacgc tgtcaatatc gccgtgatcg gtcatgttct caatcctccc
     4981 gcaacggcat gcgtgccgcc tcgtcgaaag cggcctt
//
LOCUS       DATLJT010001250         3793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1247065,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001250 DATLJT010000000
VERSION     DATLJT010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247065"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VM910_28735"
     CDS             complement(<1..375)
                     /locus_tag="VM910_28735"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI66534.1"
                     /translation="MLVNSAAVLKKYPFRLLTQAEYSNNTMRVMRTFLVVALMATAGW
                     IPACAAERVDLLLVLAADSSRSVDAVKFQLQRSGYAAAFSNPRVIDAIRGGPSRRIAV
                     TFVEWSGPLSQETVIDWTVIGDE"
     gene            493..918
                     /locus_tag="VM910_28740"
     CDS             493..918
                     /locus_tag="VM910_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66535.1"
                     /translation="MRRAFFAALWCVIAATPARAEVRILASPGGAVGPYLELFSVLRQ
                     SGQRVVIDGPCFSACTLVLSTIPSNRICVTPKAVLGFHSPRWVDRQGRQYPATAETRL
                     FAATYPARVRAWIERHGGLTSKPIPLRGRELAVMFPRCS"
     gene            complement(1887..3061)
                     /locus_tag="VM910_28745"
                     /pseudo
     CDS             complement(1887..3061)
                     /locus_tag="VM910_28745"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(3025..3270)
                     /locus_tag="VM910_28750"
     CDS             complement(3025..3270)
                     /locus_tag="VM910_28750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66536.1"
                     /translation="MPGTALEDIKLEAPRVTAPASLPFCTTEHYDRTAHTYGKSYPEL
                     ARGLARDYASAPDVVAYPRNEADVKSCSTGPAAPTRP"
     gene            complement(3275..3439)
                     /locus_tag="VM910_28755"
                     /pseudo
     CDS             complement(3275..3439)
                     /locus_tag="VM910_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          698 a         1216 c         1175 g          704 t
ORIGIN      
        1 ttcgtcaccg attaccgtcc aatcaatcac ggtctcctgt gacaaggggc cggaccattc
       61 gacgaacgtc acggcgattc ggcgactcgg tccgccgcga atggcatcaa ttacccgtgg
      121 attggagaac gccgctgcgt agccggagcg ctgaagctgg aactttaccg catcaacgct
      181 gcgcgagctg tcggcggcca gcacaagaag gagatcgacg cgctcggcgg cgcaggcggg
      241 tatccaaccc gcagtcgcca tgagcgcgac gaccaagaag gttcgcataa cgcgcattgt
      301 gttgttactg tattccgcct gcgtcaacag ccggaatgga tactttttca acacagccgc
      361 cgagttgacg agcacgtgct cgaaatccgg cgtctcaggg gctaacggat tcccttgata
      421 agccgcgagc tgctataatt ttcgaaatac ttccgatgcg gcgcgaacgg caatcgagag
      481 tggagaattg cggtgcgacg tgcgttcttt gctgcgctct ggtgcgtcat cgccgcgacg
      541 ccagctcgcg ccgaggtgcg catcctggca agtcccggcg gtgcggttgg cccatatctc
      601 gaactgttct cggtcttgcg gcaatccggt caacgagtgg tgatcgacgg gccgtgcttc
      661 tccgcctgta cgctggtgct gagcacgatc ccaagcaatc gtatctgcgt cacgccaaag
      721 gccgtactgg gctttcactc gccccgttgg gtcgaccggc agggtcgcca atatcccgcc
      781 actgcggaaa cacgcctctt cgcggccacc tatcccgccc gggtccgtgc ctggattgag
      841 cggcatgggg gtcttacgtc caagccaatc cctctgcgcg gccgagaact ggcggtgatg
      901 tttccgcgct gcagttagct acagccacag cccgccttgg tgatacggga acattctcat
      961 tttcaacccg ggcgcagagt ggctcccacc ccaacgggtc atcacgctcg accacgtcgt
     1021 cgtcgactaa ataccaaccc tcacgatagt ccccagcccc aaacgtttcc cggcagcgtc
     1081 cctcgctcgc cgccttgtca ttcttctgca caggcgtcgc ctggatttgg ggttacgtta
     1141 ttttgggttc ggtctttttt gcgctcggtg gagtcgccgc gctcggccta gccgagaacc
     1201 actggcgctg acaggcgctg acatgggcag ctaatcctca agcgcgcctc ggcttcccgc
     1261 tcgtcggcca atgcgcgggc ctgcgccgtc gtcttgcgat ctcatattcg cactgcggtc
     1321 agattagtgg ctcggcccag cagcgtccgt ccatcggcgc gaaaggggtt cgcataactg
     1381 ccagaccgag ccgggtgccg actgtcccaa agaaagtttt aacgcgaggt taaatcggct
     1441 cgatgcgagt gcgtgcagcg caacctaata ggaaccatca accatctata tatgcatcgc
     1501 ggagcccatc cgtagagctg tgttcatgct tgcaggcgcg ggcgggtacg ggctgcgcgc
     1561 caaccgtggc caaaatgctg gcacaaatct acgcttagag cttcaactgg cgactggccg
     1621 aattgcccga ctccacggtg cgcaaaagat gtttggcgcc acaagcgccc catgcgctcc
     1681 ggcgagcgca atcgtcgtta gcacggcgat gaaaccgccc aacgcgaaag cgaacgcaac
     1741 gcacctcgcg tgacgtgtaa cggtagcggc cttgcccgac gttacacgta acggcaaggg
     1801 cgccacctca cgacatgcgt aacggccccc tgatcgttta aaacccgaag cgccaagagc
     1861 tgagagaagg tctcccgtgg acagcgtcat gggtcaatga gaacgcccgg attgagcagg
     1921 ccctgcggat cgagtgcttt cttcgcggcg cgcagcgcct cggcaaacag cggcgggcgc
     1981 tgcctgtcgt accacgggcg atgatcgcgc ccgaccgcat ggtgatgcgt gacagtcccg
     2041 ccattcgcaa tcactgcgtc gagtgcccca cttttgattg cgagccattg cgcgccgagc
     2101 ttgcccggct cgccgagcgc gtggaatgtg aaatagggag cggggccgtc gggatagaca
     2161 tgcgtgaagc ggcaggtgac tcccgtgtga tcgacgtctc gaaggtgtcg ttaatcacgc
     2221 cgcgcaggat cagccgttcc atcgcgtagg gcatacggat gaaggcgttg cgccacacgc
     2281 cagcggcgcc ctcacggtgc gcccctgcgc cgctcgcctc gggcgtgccg ccgtgatcgg
     2341 cgcagcattc gcgggcgcgc ttcatccacg gcgcgacatc gtggtccgcc gactcgaagg
     2401 ccagcaccat gatggctacc gagccatctg ccgcgccggt gttgtatgcc tcggtcgaat
     2461 caaggatgcg gcagttcgag ggatagaggc cggcctgaga gatcgcgcgc acggcgcgtg
     2521 ctgcggaaaa gaaatccttg aaacgcaccg caccgccgtc ccgaaatttc gggcggtcct
     2581 gcaagcgcat ccacgcctgg gtgatgacgc cgagaattcc ttccgagccg atcatcatgc
     2641 ggtccgggct cgggcctgcg ccggagccgg ccaggcggcg gctttcgatc aggcctctcg
     2701 gcgtgagggt acgcagcgat tcaacgaagt catcgatgtg cgtatagagc gtcgcgacgt
     2761 ggccgccgcc gcgcgtcgca atccagccgc cgagcgttga atattcgaag ctctgcggga
     2821 aatgacgtag cgtgagcccg tgcggccgaa gctgcgcttc gagcgcgggt ccgtaggtgc
     2881 cgccctcgat aagcgcggcg cgcgaagtct tgtcgacctc cttcaccttg tccatttggc
     2941 gcaggtcgat cgtgaccgca cccttgtggc cgttgcgctt cggctcgacg cccccgcaga
     3001 cgcttgaccc accgccgaac ggtgtcacgg tcgcgtcggc gccgccggcc cagtcgagca
     3061 cgacttcacg tccgcctcgt tgcgcggata ggcgactacg tcgggagcgc tcgcataatc
     3121 gcgtgcaagg ccgcgggcga gttcggggta tgatttaccg taggtgtgcg cggtacggtc
     3181 gtagtgttcg gtggtgcaga acgggagcga ggcgggcgcc gtgacgcgcg gcgcttcgag
     3241 cttgatgtct tcaagtgccg tccccggcaa tgaccggcag atcaccctcg ggcaggcgca
     3301 ccgcacgccg gagcttacgc gtcgacacgt tgatcagcat caggttcatg gcccagcggc
     3361 caagccagtc ctcggcctgt gccgccgccc aggtcggcag cgcaacttca tggccgtcgt
     3421 agctgcgcac ccgcgggcga gcctagccca ttgcgagcag ctccagcggc ctgctaacgg
     3481 tcatgctcga tctcatgcag ccacgacttc gacgtgcttg ccgatggccg caacaagcag
     3541 aaacgccgag cactagcgac tagtacaaat tcaaatattg agcgcggtaa atcgcgtcgc
     3601 tcgacgggat cggcgcagcc gtatcattcg caggatacgg gggagcagct aacgtcatga
     3661 ccactaggat gatcaccgcc aacgcaagga ccgcggcgaa cctaactgcc aggctgtctg
     3721 gttcccgtgc ctcagatccg ttacatcgcg tttgcacgtg acagcctcct tcgtcggctg
     3781 catcggtgaa gcc
//
LOCUS       DATLJT010001251         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3544650,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001251 DATLJT010000000
VERSION     DATLJT010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544650"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VM910_28760"
     CDS             <1..613
                     /locus_tag="VM910_28760"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HVI66537.1"
                     /translation="SIAYRPACDQCRACVSVRVIANEFRPSRNLRKVLARNADIVGEQ
                     RSAVPTSEQYSIFRAYLDARHRHGGMADMTVLDYAMMVEDSHVETRIIEYRRRNSDRG
                     IFGRGDELLAVALTDVLSDGLSMVYSFFEPSEDSRSLGTFMILDHIARARRQGLPYVY
                     LGYWIEGSKKMDYKGRFLPQQRLAPSGWLRIDASGDTMTEPQD"
     gene            complement(610..1995)
                     /locus_tag="VM910_28765"
     CDS             complement(610..1995)
                     /locus_tag="VM910_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HVI66538.1"
                     /translation="MDARPSDLSLEPSRAEGASSGQPGWRGARKEPSLSDVFASVRTA
                     PAGGSFWRKLLAFLGPGYLVAVGYMDPGNWATSLAGGSKFGYALLTVALLSNVMAIVL
                     QSLCTRLGVGAGRDLAQACRDSFPRWVSFPLWLSAEVAITATDLAEVIGTAIGLNLLF
                     HIPLEIGVIITALDVFLILALQAFGFRWIEAFVVAMLGVIAICFAMQIALADPDWGAV
                     IRGFAPSRDLLANREMLYLALGILGATVMPHNLYLHSGLVQTRVFGETPAEKREAITL
                     ATIDSSIALCFAFVINASILILAAATFHRAGKTDIAELAQAHSFLAPLLGSTLAPTLF
                     AVALLCCGLNSTITATLSGQIVMEGFLKFRIAPWLRRLVTRVIAILPAVVVTIYAGEK
                     ATGQLLILSQVVLSLQLPFAVVPLVLFTASRTKMGPYVAPRWLTLLAAAIAAAIIVLN
                     AKLVFDFVSGS"
BASE COUNT          499 a          752 c          759 g          383 t
ORIGIN      
        1 gtcgatcgcc tatcggccgg cctgcgacca gtgccgggcc tgcgtctccg tgcgggtgat
       61 cgccaacgaa ttccgtccct cgcgcaatct tcgaaaggtc ctggcccgca acgccgacat
      121 cgtcggcgag cagcgcagcg ctgtgccgac ctcggagcaa tattccatct tccgcgccta
      181 cctcgatgcc cgccaccgcc atggcggcat ggccgacatg accgtgctcg actacgcgat
      241 gatggtggag gacagccatg tcgaaacgcg tatcatcgaa taccgcaggc gaaacagcga
      301 cagaggcatc ttcggccgcg gcgatgaact gctggcggtg gcgctgacgg atgtgctcag
      361 cgacgggctg tcgatggtct attcgttctt cgagccctcg gaggacagcc gctcgctcgg
      421 taccttcatg atcctcgacc acatcgcgcg ggcgcggcgg caaggcttgc cgtacgtcta
      481 tctcggttac tggatcgagg gctccaagaa gatggactat aagggccgtt tcctgccgca
      541 acagcggctt gcgccctcgg gctggctgcg catcgatgca tccggcgata cgatgaccga
      601 gccgcaggat tagctgccgc tcacgaaatc aaacaccagt ttcgcattca gcacgatgat
      661 cgctgcagcg attgccgcgg caagcagggt gagccagcgc ggcgccacgt agggacccat
      721 ttttgtcctg cttgcggtga acagcacgag cggcaccacg gcaaacggca attgcaggct
      781 gagcacgacc tggctcagga tcaggagctg cccggtcgcc ttctcgccgg catagatggt
      841 cacgacgacc gccggcagga tcgcaatgac gcgcgtcacc agccgccgca gccagggcgc
      901 gatgcgaaac ttcaaaaatc cctccatcac gatctggccc gacagtgtcg cggtgatggt
      961 cgagttcaac ccgcagcaga gcaacgcgac tgcaaacagg gtcggggcca gggtcgagcc
     1021 gagcagcggc gcgagaaaag aatgtgcctg ggcgagttcg gcgatgtcgg ttttccctgc
     1081 acgatggaag gtcgcggcag ccaggatcag gatcgaggcg ttgatcacga aggcgaagca
     1141 caatgcgatc gaagaatcga tggtggccaa tgtaatggcc tcgcgcttct cggccggcgt
     1201 ttcgccgaag acccgggtct gcaccagccc cgaatgcaga tagagattgt gcggcatcac
     1261 ggtcgcgccg aggatgccga gcgcgagata gagcatctcg cggttggcga ggagatcgcg
     1321 gctcggtgca aagcccctta tgaccgcacc ccaatcggga tcggcgagcg cgatctgcat
     1381 cgcaaagcag attgcgatca cgcccagcat cgctaccacg aaggcctcga tccagcggaa
     1441 gccgaacgcc tgcagcgcga ggatcagaaa gacatcgagc gccgtgatga tcacgccgat
     1501 ctcgagggga atgtgaaaca agagattgag gccgatcgcg gtgccgatca cctcagcaag
     1561 atcggtcgcg gtgatcgcaa cctccgccga tagccagagc ggaaagctga cccatcgcgg
     1621 aaaggaatcg cggcaggcct gcgccaggtc gcggcccgcg cccacgccaa gccgcgtgca
     1681 cagcgattgc agcacgatcg ccatcacgtt ggagagcagc gcaaccgtca gcagcgcgta
     1741 gccgaatttg gagccgccgg cgagcgaggt cgcccagttg cccggatcca tatagccgac
     1801 ggcgacgagg tagccgggac ccaggaaggc caggagcttg cgccagaacg atccaccggc
     1861 gggcgccgtc cggaccgacg caaaaacgtc tgagagtgat ggttccttgc gcgcgccccg
     1921 ccagcccggc tggcctgacg aagcgccttc tgcgcgcgag ggttccaacg acagatcaga
     1981 gggtcgggca tccatcggcc cagaatgtcg gaaaactgac tttaatgcaa ctcattcgca
     2041 actgcatcta ggatgctgtg ttccaacggg ctggcctagg cgacttcgcg ccgccgtcca
     2101 gtgaaaagca cgatacgcat gcctgcgtgc ggcgttgtcg gctcaagaac tcggaccgcg
     2161 tggtgcactg ccagtgatca gcatgagcat ttttccggtg gcgtttggca tcgaagcgat
     2221 ccgtaccggc ttgaagcggc gccacccgaa tcctgacaaa cctggcggtg aacccacgaa
     2281 gtcatcgtcg gaaatcgggt gggtctgagc ctgctcggcg cccatgttgc ggccaaccac
     2341 gcaacaggag ccacgcatgg ctggaccgca aaagaccctg ccgccgacct tca
//
LOCUS       DATLJT010001252         5361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1707131,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001252 DATLJT010000000
VERSION     DATLJT010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707131"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VM910_28770"
     CDS             <1..639
                     /locus_tag="VM910_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HVI66539.1"
                     /translation="PALHVDTGKKFPEMYAFRDHYGKEWDLDLRVEPCPPIDAVDPTL
                     PPAARSAARKTEGLKMALAKYGFDGLIAGIRRDEEATRAKERVFSPRGLEGGWDVRDQ
                     PPEFWDQFNASPPPGAHLRVHPILHWTEADIWAYTRRESIPIIPLYLSRNGKRYRSLG
                     DADITFPVVSDASNIEEILAELEATRIPERAGRALDHETEDAFERLRVAGYL"
     gene            671..2587
                     /gene="cysC"
                     /locus_tag="VM910_28775"
     CDS             671..2587
                     /gene="cysC"
                     /locus_tag="VM910_28775"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HVI66540.1"
                     /translation="MNMILPMSLSTTPNGTTRPQVRIVIVGHVDHGKSTLVGRLLHET
                     GSLPEGKLEMLKAVSARRGMPFEWSFLLDALQTERDQGITIDTTQIRFRTNSRDIVLI
                     DAPGHAEFLRNMITGASQADGAVLIIDALEGVRDQTRRHGYLLHLLGIKQVAIVVNKM
                     DRVEFQKARFDEIAAEISAHLTNLGVTPSAVIPISARDGDGVAARTPRIGWYKGPTVV
                     EALDALEPARPLEALPLRLPVQAIYKFDDRRIVAGRIESGHLGAGDEIVIMPAGKIAK
                     IRTVESWPLTPVAGKQCAGRSVGITLDRELFVERGDIIAPVGRAPRDTRRVHARIFWL
                     HEKPLAKGDTILVRLGNKETRASVVAIENAVDPGDLASHENTAIAKNHVGEIDIALAQ
                     PIAADAYADNPRTGRLVIEVSGRIAGGGLVLSVDAGRRAVPVDIVPVESALRSDERSA
                     RYRHSGAVVWLTGLPGAGKSTLARALERRLFSRGGSPILLDGDTLRAGLNGDLGFSPK
                     DRGENIRRLAEVATHLAKNGHIAIVAAVSPSRDDRESARRIADDTFREVYVATPAEIC
                     ESRDPKGHYAKARAGTLKGFTGIGNDYEPPASAELTLDTSIGSVTDAADEIERMLAKT
                     GILFDELVDLAANI"
     gene            2753..4918
                     /locus_tag="VM910_28780"
     CDS             2753..4918
                     /locus_tag="VM910_28780"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HVI66541.1"
                     /translation="MKRIEAHGLKIAPVLFDFIAKEAAPKTGIDPDAFWKGLAAIIRD
                     LAPRNRELLAVRDTLQGKIDGWHRANKGKPVDIAAYTSFLKEIGYLLPEPATQKVETA
                     KVDEEIGKICGPQLVVPLTNARYALNAANARWGSLYDAFYGTDAIPHDPSESGKGYSK
                     ARGDKVIAKAKAFLDSAAPLATASHTDVTSYSIVAGKLAMKLKSGNATALKSNQQFAG
                     YQGDPAAPSTILLVNNGLHIEIKIDRKHPIGKDDSAGVADMMLEAAVSTILDMEDSVA
                     AVDAEDKVLIYRNTLGLMNGTLSADFEKGGKTMTRALNPDRVYKTPDGKELKLHGRSL
                     LLMRNVGHHMFTDAVLDEKGEEIPEGILDAAVAGLLAIHDLKGNSKVRNSRTGSVYIV
                     KPKMHGPDEVALTCAIFDRVEKLLALPADTLKVGIMDEERRTTVNLKACIQQALRRIV
                     FINTGFLDRTGDEIHTSMEAGPMIRKNEMKAQPWIKAYEDWNVDIGLIDGLPGHAQIG
                     KGMWAAPDKMADMLAQKIGHPQAGATTAWVPSPTAATLHALHYHQVNVLARQQELAKG
                     GPRAKLSDILTIPVSQSNWAPDDVKQEIDNNCQSILGYVVRWIDQGVGCSKVPDIHDV
                     GLMEDRATLRISSQHLANWLHQGVITKDQVMESLKRMAVVVDKQNAGDPLYKPMAPSF
                     DGVAFKAACDLVFKGREQPNGYTEYILTARRREAKAADR"
     gene            4991..5191
                     /locus_tag="VM910_28785"
     CDS             4991..5191
                     /locus_tag="VM910_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HVI66542.1"
                     /translation="MDWNRIEGNWKQLKGNVKERWGKLTDDDLNVIEGRREQLEGKLQ
                     QRYGYAKDQAHKDVDDWFKTLK"
     gene            complement(5251..>5361)
                     /locus_tag="VM910_28790"
     CDS             complement(5251..>5361)
                     /locus_tag="VM910_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HVI66543.1"
                     /translation="LYLINMLPLDGRARQICRVIVIILGVLSLLKYLAVY"
BASE COUNT         1089 a         1755 c         1655 g          862 t
ORIGIN      
        1 ccggcattgc acgtcgacac cggcaagaaa tttccggaga tgtacgcgtt ccgcgatcac
       61 tacggcaagg agtgggacct ggatctgcgc gtcgagccgt gcccgccaat cgatgcggtc
      121 gatccgaccc tgccgccggc tgcgcgctcc gcggcgcgca agaccgaggg gctgaagatg
      181 gcgctggcca agtacggctt cgacggattg attgccggca tccgccgtga cgaagaagcc
      241 acccgcgcca aggagcgcgt gttttcgccg cgcggcctcg agggcggatg ggacgtgcgc
      301 gatcagccgc cggagttctg ggatcagttc aacgcgtcgc cgccgcccgg cgcacatttg
      361 cgcgttcacc cgatcctgca ttggaccgaa gccgatatct gggcctacac caggcgcgag
      421 agcatcccga tcatcccgct ttatctctcc aggaacggca agcgctaccg ctcgctcggc
      481 gatgccgaca tcacttttcc ggtggtgtcg gacgcctcga acatcgagga aatcctggcc
      541 gagctcgaag ccaccaggat acccgagcgg gccgggcgcg cgctcgatca tgaaaccgaa
      601 gacgctttcg agcggctcag ggttgccggc tatctctgat caacatcaat cattcggcac
      661 gagcgtcgtc atgaacatga tccttcccat gagcctctcc acaacgccga acggcaccac
      721 gcgcccgcag gtccgcatcg tcatcgtcgg ccatgtcgac cacggcaaat ccacgctcgt
      781 aggccgcctc ctgcacgaaa ccggcagcct gcccgagggc aagctcgaga tgctgaaggc
      841 cgtcagcgcg cggcgcggca tgcccttcga atggtccttc ctgctcgatg cgctgcagac
      901 cgagcgcgac cagggcatca ccatcgatac cacgcaaatc cgctttcgca ccaattcgcg
      961 cgacatcgtg ctgatcgacg cgcccggaca tgccgagttc ctgcgcaaca tgatcaccgg
     1021 cgcgtcgcaa gccgacggcg ccgtgctgat catcgacgcg ctcgaaggcg tgcgcgatca
     1081 gacccgccgc cacggctacc tcctgcactt gctcggcatc aagcaggtcg cgatcgtcgt
     1141 caacaagatg gaccgcgtcg agtttcagaa ggcgcgattt gacgaaatcg ccgccgagat
     1201 ctcggcgcac ctgacaaatc tcggcgttac gccctcggcg gtcattccga tctccgcgcg
     1261 ggacggcgac ggcgttgcgg cgcgcacgcc gcggatcggc tggtacaagg ggccgaccgt
     1321 ggtcgaggcg ctggatgcgc tcgagccggc gcggccgctc gaggccttgc cgctgcggct
     1381 gccggtgcag gcaatctaca aattcgacga tcggcgcatc gtcgccggcc gcatcgagtc
     1441 cgggcatctt ggcgcgggcg acgagatcgt catcatgccc gcaggcaaga tcgcaaaaat
     1501 caggacggtc gaaagctggc cgctgacgcc ggttgcagga aaacagtgcg ccgggcgctc
     1561 cgtcggcatc acgctcgacc gcgagctgtt cgtcgagcgc ggcgatatca tcgcgcccgt
     1621 cggtcgcgcg ccgcgcgata cgcgacgggt ccacgcgcgg attttctggc tgcacgagaa
     1681 gccgctcgca aaaggcgaca ccattctggt gcgcctcggc aacaaggaga cgcgcgccag
     1741 cgtcgttgcc atcgagaatg cggtcgatcc cggcgatctt gcaagccacg agaacacggc
     1801 gattgcgaag aaccacgtcg gcgagatcga catcgcgctg gcccagccga tcgccgccga
     1861 cgcctatgcc gacaacccgc gcaccgggcg gctggtcatc gaggtttcgg gccgcatcgc
     1921 gggcggcggg cttgtgctgt cggtcgacgc cggccggcgc gccgttcccg tcgacatcgt
     1981 gccggtggaa tcggcgctgc gttccgacga acgctcggcg cgctatcgcc acagcggtgc
     2041 ggtggtctgg ctgaccggcc tgccgggcgc cggcaaatcg acgcttgcgc gcgcgctgga
     2101 gcggcggctg ttcagccgcg gcggctcgcc cattttgctc gacggcgaca cgctgcgcgc
     2161 gggtctcaac ggcgatctcg gcttttcgcc gaaggaccgc ggcgagaaca tccgtcgtct
     2221 cgccgaagtc gcgacgcatc ttgcgaagaa cggccatatc gcgatcgtcg cggcggtctc
     2281 gccctcgcgc gacgatcgcg agtccgcccg gcgtatcgcc gacgatactt tccgcgaggt
     2341 ctatgtcgca acgccggcgg agatctgcga gagccgcgac cccaaaggtc actatgccaa
     2401 ggcacgcgcc ggcacgctca aaggcttcac cggcatcggc aacgactatg agccgccagc
     2461 atcggccgaa ttgacgctcg atacctcgat cggttcggtg acggatgccg ccgacgagat
     2521 cgaacgcatg ctggccaaaa ccggcattct gttcgacgaa ctggtcgatc tcgccgccaa
     2581 tatctgacct caccccgtca gccgttttac taggcacgga acgctcgcca ggggccttcc
     2641 taagcggacg gctttggggc taaaagagcc tttcccgccg cgccagcgcg gcgctttttt
     2701 gctgttttcg gcctgaaaac agccattcat caccgttctt gagaaggccg acatgaagcg
     2761 tatcgaagcc cacggattga agattgcccc tgttctgttc gatttcatcg caaaggaagc
     2821 cgcgcccaaa accgggatcg atcccgatgc gttctggaag ggacttgctg caatcatccg
     2881 cgatctcgcg ccgcgtaatc gcgaattgct cgccgttcgc gacacgctgc aaggcaagat
     2941 cgacggctgg catcgcgcca acaaaggcaa gcccgtcgac attgccgcct atacctcctt
     3001 cctgaaggaa atcggctacc tgctcccgga gcctgcgacg cagaaggtcg agacggcgaa
     3061 ggtggatgag gagatcggca agatctgcgg gccgcagctc gtcgtgccct tgaccaatgc
     3121 ccgttatgcg ctgaacgcgg cgaacgcacg ttggggctcg ctctatgacg ccttctacgg
     3181 caccgatgcg atcccccacg atccgagcga aagcggaaag ggttacagca aggcgcgcgg
     3241 cgacaaggtg atcgccaagg caaaggcctt tctcgattcc gccgctccgc ttgcgacggc
     3301 aagccacacc gacgtcacct cctacagcat cgtcgcgggg aagcttgcga tgaagctgaa
     3361 gagcggaaat gccaccgcgc tgaaatcgaa ccagcaattt gccggctatc agggcgatcc
     3421 cgccgcgccg tcgacaatcc tgctggtcaa caacggcttg cacatcgaga tcaagatcga
     3481 tcgcaagcac ccgatcggca aggacgactc cgccggcgtc gccgacatga tgctcgaagc
     3541 cgctgtctcg accatcctcg acatggagga ttccgtcgct gccgtcgacg ccgaggacaa
     3601 ggttctgatc taccgcaaca cgctcggcct gatgaacgga acgctgtcgg ccgatttcga
     3661 aaagggcggc aagaccatga cgcgcgcgct caatcccgac cgcgtctaca aaacgcccga
     3721 cggcaaggag ctgaagctgc acggccgcag cctgcttctg atgcgcaatg tcggccatca
     3781 catgttcacc gacgcagtcc tcgacgaaaa gggcgaggag attccggaag gcattctgga
     3841 tgccgccgtc gccggtctgc tcgccatcca tgacctgaag ggcaactcga aggtcaggaa
     3901 cagccgcacc ggctcggtct acatcgtgaa accgaagatg catggccccg acgaggtcgc
     3961 gttgacctgc gcgatcttcg accgcgtcga gaaactgctc gcgctgcccg cagacacgct
     4021 gaaggtcggc atcatggacg aagagcgccg caccaccgtc aacctgaagg cctgtatcca
     4081 acaggcgctc aggcgcatcg ttttcatcaa cacaggcttc ctcgatcgca ccggcgacga
     4141 gatccatacg tccatggaag ccggcccgat gatccgcaag aacgagatga aggcgcagcc
     4201 ctggatcaag gcctatgagg actggaacgt cgacatcggc ctgatcgacg ggctgccggg
     4261 tcacgcccag atcggcaagg gcatgtgggc ggcgcccgac aagatggccg acatgctggc
     4321 gcagaaaatc ggccatccgc aggccggcgc caccacggcc tgggtgccct cgccgactgc
     4381 agccacgctg catgcgctgc actatcacca ggtcaatgtg ctggcccggc agcaagagct
     4441 cgccaagggt gggccgcgcg cgaaactgtc ggacatcctc acaatcccgg tgtcacagtc
     4501 gaactgggcg cccgacgacg tgaagcagga gatcgacaac aactgccaga gcattctggg
     4561 atacgtcgtg cgctggatcg atcagggcgt tggctgctcc aaggtgcccg atatccacga
     4621 tgtcggtttg atggaggatc gcgcgaccct gcgaatctcc agccagcatc tggcgaattg
     4681 gctgcatcag ggcgtcatca ccaaggatca ggtgatggaa tccttaaaac gcatggcggt
     4741 cgtcgtcgac aagcagaacg ccggcgatcc cctgtacaag ccgatggcac cgtcgttcga
     4801 cggcgtggcg ttcaaggcgg cctgcgatct cgtcttcaag ggacgcgagc aaccgaacgg
     4861 ttacaccgaa tacatcctca cagcgcgccg ccgcgaggcc aaggcggctg accgatgatc
     4921 cctcgccgga accggaacgt gtgcggcgtc gtcccgttgt ccatcaactc aaccgacaac
     4981 ggagatggca atggattgga accggatcga aggcaactgg aaacagctca agggcaacgt
     5041 gaaggagcga tggggcaagc tcaccgacga tgatctcaac gtcatcgaag gccgccgcga
     5101 gcaactcgag ggcaagctgc agcagcgcta cggttacgcc aaggaccagg cccacaagga
     5161 cgtggacgac tggttcaaga cgctgaagta gtgcgtatca ccgcaacaaa agccccggca
     5221 aaagccgggg ctttttcatt gaggcgaacc tcagtacacc gcgagatatt tcaacagcga
     5281 gagcacgccg aggatgatga cgatcacgcg gcaaatctgc cgggcgcggc cgtccagcgg
     5341 caacatgttg atgagataga g
//
LOCUS       DATLJT010001253         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3085313,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001253 DATLJT010000000
VERSION     DATLJT010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3085313"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..>2139
                     /gene="leuS"
                     /locus_tag="VM910_28795"
     CDS             146..>2139
                     /gene="leuS"
                     /locus_tag="VM910_28795"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HVI66544.1"
                     /translation="MANDRYNARDAEARWQKIWDERGIFLTRNDDPRPKYYVLEMFPY
                     PSGRIHMGHVRNYTMGDVVARVKRAMGLNVLHPMGWDAFGMPAENAAMERKVHPRAWT
                     YDNIAAMKTQLQSMGLSLDWSREIATCDPRYYKHQQKMFLDFLRAGLVERKKSKVNWD
                     PVDQTVLANEQVIDGRGWRSGALVEQRELTQWFFAITKFAAPLLEALDKLDRWPEKVR
                     LMQKNWIGRSEGLLVRFALDPASAPNGETELEVFTTRPDTLFGAKFMALAPDHPLAAA
                     AAKHNPALAEFIEQCKHRGTAQAEIETAEKLGFDTGIRVRHPFEAAWELPVYVANFIV
                     MEYGTGAIFGCPAHDQRDLDFVNKYGLGNVPVVCPPGVDPKTFVITDTAYDGDGTMIN
                     SRFLNGMTSAAAKEEVARRLEQERRNNRPVAERQVNYRLRDWGISRQRYWGCPIPIIH
                     CEHCGTVPVPEKDLPVTLPEDVTLDRPGNPLDHHPTWKHVACPQCGGPARRETDTMDT
                     FVDSSWYFERFTDPWIETAPTDRPMVDAWMPVDLYIGGIEHAILHLLYSRFFTRAMRE
                     TGHVGLDEPFAGLFTQGMVVHETYRRADGEWAAPAEVKVESDGDNRRATFATTGEAVE
                     IGPIEKMSKSKRNTVDPDDIIETYGADVARWFMLSDSPPER"
BASE COUNT          418 a          689 c          669 g          363 t
ORIGIN      
        1 gaacgaacac gcaagaatcg gcgctggcgg gtgcaatcgc tcgcccgcca tgcaccgccc
       61 gttcacggtt ttgctctaag acctgttgtt gacccgccgc gcgatttcgt ggcacctgcc
      121 cgcggcagaa atactcttaa acctgatggc caacgaccgc tacaacgccc gcgacgccga
      181 ggcgcgctgg caaaaaattt gggacgagcg cggcatcttc ttaacccgca acgacgaccc
      241 gcggccgaaa tactacgtgt tggaaatgtt cccctatccg tcggggcgca tccatatggg
      301 ccacgtgcgc aattacacca tgggcgacgt ggtggcgcgg gtgaagcggg cgatgggatt
      361 gaacgtgctg cacccgatgg gctgggacgc cttcggcatg ccggccgaga acgccgcgat
      421 ggagcgcaag gtccatccca gggcgtggac ctacgacaat atcgcggcga tgaagacgca
      481 gctccaatcc atggggctgt cgctcgactg gagccgcgaa atcgcgacct gcgacccgcg
      541 ctattacaag caccagcaga aaatgtttct cgatttcctg cgcgccggcc tggtcgagcg
      601 caagaaatcg aaggtgaact gggacccggt cgaccagacg gtgctggcga acgagcaggt
      661 gatcgacggc cgcggctggc gctccggcgc cctggtcgag cagcgcgagc tgacgcaatg
      721 gtttttcgcc atcaccaaat tcgccgcgcc tttgctggaa gcgctcgaca aactcgaccg
      781 ctggccggaa aaagtgcggc tgatgcagaa gaactggatc ggccgctccg aaggcctgct
      841 ggtccgcttc gcgctcgatc cggcgagcgc gccgaacggc gaaaccgagc tcgaagtctt
      901 taccacgcgc ccggacacgt tgttcggcgc caagttcatg gcgctggcgc cggatcatcc
      961 gctcgccgcg gcagccgcga agcacaatcc ggcgctcgcc gaatttatcg agcaatgtaa
     1021 gcaccgcggt actgcgcagg ccgagatcga gaccgccgag aagctcggct tcgacaccgg
     1081 cattcgcgtg cgccacccgt tcgaggcggc gtgggagctg ccggtctatg tcgccaattt
     1141 cattgtcatg gaatacggca ccggcgcgat tttcggctgt ccggcgcacg accagcgcga
     1201 cctcgatttc gtcaacaaat atgggctcgg caatgtgccg gtggtgtgcc cgccaggcgt
     1261 cgacccgaag actttcgtca tcaccgatac cgcctatgac ggcgacggca cgatgattaa
     1321 ttcgcgtttc ctcaacggca tgaccagcgc ggcggccaag gaagaagtcg cgcgccggct
     1381 cgagcaggag cgccgcaaca atcgcccggt tgcggagcgt caggtcaatt accggctgcg
     1441 cgattggggc atttcgcggc agcgctattg gggctgcccg attccgatca tccattgcga
     1501 gcactgcggc accgtcccgg tgccggaaaa agatctgccg gtgacgctgc cggaagacgt
     1561 caccctcgac cggcctggca atccgctcga ccatcatccg acctggaaac atgtcgcctg
     1621 cccgcaatgc ggcggcccgg cacggcgcga aaccgacacg atggatacgt tcgtcgattc
     1681 gtcctggtat ttcgagcgct tcaccgatcc ctggatcgag accgcaccga ccgatcggcc
     1741 gatggtcgat gcctggatgc cggtcgacct gtatatcggc ggcatcgagc acgcgatttt
     1801 gcatctgctc tatagccgct tcttcacccg cgccatgcgg gagaccggtc atgtcggact
     1861 cgacgagccg ttcgccggat tgttcaccca aggcatggtc gtgcacgaga cctatcgccg
     1921 ggccgacggc gaatgggcgg caccggccga agtgaaagtc gaatctgacg gcgacaatcg
     1981 ccgcgccact ttcgccacga ccggcgaggc ggtcgagatc ggcccgatcg agaaaatgtc
     2041 gaagtcgaag cgcaacaccg tcgatccgga cgacatcatc gagacctacg gcgccgacgt
     2101 cgcgcgctgg ttcatgctgt cggattcgcc gccggagcg
//
LOCUS       DATLJT010001254         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_784891,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001254 DATLJT010000000
VERSION     DATLJT010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784891"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VM910_28800"
     CDS             complement(<1..345)
                     /locus_tag="VM910_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI66545.1"
                     /translation="MLTEASTYEELYRNFRWDIPARFNIATACCDRHADGSGRLALIY
                     VDEDGRTTRTSFDEVAEQSRRFANVLKADGLKPGDRIAVFLSQSLELPIAHFAAFRSG
                     MISIPLFALFGED"
     gene            complement(354..1877)
                     /locus_tag="VM910_28805"
     CDS             complement(354..1877)
                     /locus_tag="VM910_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI66546.1"
                     /translation="MADEKAPPAGPDLSRGVALSEFSGAMLLGHVGEEEVLLVRSGAE
                     IFAIDAHCSHYHGPLADGLVAGESIRCPWHHACFDLRTGEASRAPALGALAVWQVERE
                     GDRVFVRRKREQPKPRIKGVVDAPGRIVIVGGGAAGFAAAEMLRRQEFRGRITMLSND
                     TAPPVDRPNLSKDYLAGSAPEDWLPLRPDDFYADASIELRLKTDVVAIDTKAKSVTVA
                     GGETIPYDRLLLATGAEPVRLPIPGADQPHVHTLRSLADCRAIIGSTEGAQRAIVIGA
                     SFIGLEVAASLRARGIEVHVVAPEARPMERVLGPDMGDFVRGLHEEHGVVFHLGDTVT
                     AIDGKRATLKSGGVLEGDLVVVGVGVRPRLALAEKAGLAIDRGVTVDAYLETSVADIF
                     AAGDIARWPDRHSGENIRVEHWVVAERQGQTAARNMLGQREAFDAVPFFWSQHYDVPI
                     NYVGHAETWDEIAVDGNIAARDCLLRYRKKGRTLAVASIYRDLASLEAELAMEVATP"
     gene            complement(1903..2358)
                     /locus_tag="VM910_28810"
     CDS             complement(1903..2358)
                     /locus_tag="VM910_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVI66547.1"
                     /translation="MLRDDINNAVKEAMKAKEERKLSTLRMVNSTIKNADIEARGQGK
                     PQLSDAELLGVLQKMIKQRQEAIELYDKGGRAELAAQEREEIAVISAYLPRQMSEDDV
                     KAAISAAIAETNAAGMKDMGKVIGVLKAKYTGQMDFSKASSLVKAALTG"
     gene            2546..>2788
                     /locus_tag="VM910_28815"
     CDS             2546..>2788
                     /locus_tag="VM910_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HVI66548.1"
                     /translation="MTTPENDAAWPDHKPTALLVLADGTVLQGFGFGAEGQAVGEVCF
                     NTAMTGYEEILTDPSYAGQIITFTFPHIGNVGTNEED"
BASE COUNT          480 a          959 c          846 g          503 t
ORIGIN      
        1 gtcctcgccg aacagcgcaa acaacggaat cgaaatcatg ccggagcgga acgcggcaaa
       61 atgcgcgatc ggcaactcca gcgattgcga caggaacacc gcgatgcggt cgcccggctt
      121 gagcccgtcc gccttcagca cattggcaaa gcgacgcgac tgctcggcca cctcgtcgaa
      181 cgacgttcgg gtcgtgcggc cgtcctcgtc gacatagatc agcgccagcc ggcccgaacc
      241 atcggcatgg cgatcacagc aggcggtcgc gatgttgaat cgcgcgggaa tgtcccagcg
      301 gaaattgcgg tagagctctt cgtaggtgga ggcttcggtg agcatgaccg ttcctagggc
      361 gtggccacct ccatcgcaag ctcggcctcg aggcttgcca gatcgcgata gatcgaggcg
      421 acggcgagcg tgcgtccctt tttcctgtac cgcagcaggc aatcccgggc ggcaatattg
      481 ccgtcgacgg cgatttcgtc ccacgtctcg gcgtgaccga cataattgat cggcacgtcg
      541 tagtgctggc tccagaaaaa cggcaccgcg tcgaatgcct cgcgctgtcc cagcatgttg
      601 cgcgccgcgg tctggccctg gcgttccgcc accacccaat gctcgacgcg gatgttctcg
      661 ccggaatgcc gatccggcca ccgcgcgata tcgcccgccg caaagatatc agcgacgctg
      721 gtttcgagat atgcgtcgac cgtgacgccg cgatcgatcg caagtcccgc cttctcggcc
      781 agcgcgagac gcggccgcac gccgacaccg acgacaacga ggtcgccctc cagcacgccg
      841 ccgctcttca gtgtcgcgcg cttgccgtcg atcgccgtca cggtatcgcc gaggtgaaaa
      901 acgaccccgt gctcctcgtg caggccacgc acgaagtcgc ccatatccgg gccgagcacg
      961 cgttccatag gacgcgcttc gggggccacg acatgaacct cgatgccgcg cgcgcgcagc
     1021 gacgccgcga cctcgagccc gataaagctc gcgccgatca cgatcgcgcg ctgcgcgccc
     1081 tccgtcgatc cgatgatcgc gcggcagtct gccagcgaac gcaaggtgtg gacatgcggc
     1141 tggtccgccc ctggaatcgg cagtcgtacc ggttccgcgc cggtcgcaag cagcaagcgg
     1201 tcatacggga tcgtttcgcc gcccgcgacg gtcacgcttt ttgccttggt gtcgatggcg
     1261 acaacatcgg ttttgagccg cagctcgatc gatgcgtcgg cgtagaaatc gtcgggtcgc
     1321 agcggcagcc agtcctccgg tgcgctgccc gcgagataat ccttcgacag gttcggccga
     1381 tccacgggcg gcgctgtgtc gttgctcagc atggtgatgc gcccgcgaaa ctcctgccgt
     1441 cgcagcatct cggcggccgc aaatcccgcc gccccgccgc cgacaatcac gatgcgcccg
     1501 ggcgcgtcga cgacgccctt gatccgcggc ttcggctgct cgcgcttccg ccgcacgaac
     1561 acccggtcgc cctcgcgctc gacctgccag acggccagcg cgccgagcgc cggagcccgg
     1621 cttgcctccc cggtgcgcaa atcgaaacag gcgtgatgcc aggggcagcg gatgctttcg
     1681 ccggccacca ggccgtctgc gagcggcccg tgatagtggc tgcaatgggc atcaatggcg
     1741 aagatttcgg ccccggaacg caccagcagg acttcttcct cgccgacatg gccgagcagc
     1801 atcgcgccgg agaactcaga aagggcaacg ccccgggaga ggtcggggcc ggcaggtggg
     1861 gccttttcat ccgccatggg ttcctccgct atgtcttgat ggtcaccccg tcagtgccgc
     1921 cttcaccagc gagcttgcct tcgaaaaatc catctgtccc gtgtatttcg ccttcaagac
     1981 gccgatcacc ttgcccatgt ccttcattcc ggcggcattg gtctcggcga tcgcagccga
     2041 gatcgcggct ttcacgtcgt cctccgacat ctgcctgggc aaataggccg agatgaccgc
     2101 gatctcctcg cgctcctggg cggcgagctc ggcgcgtccg cccttgtcat agagctcgat
     2161 cgcctcctga cgctgcttga tcatcttctg cagcacgccg agcagttccg catccgaaag
     2221 ctgtggcttg ccctgcccgc gcgcctcgat gtcggcgttc ttgatggtcg aattgaccat
     2281 gcgcaacgtc gagagttttc gttcctcctt ggccttcatg gcctccttca ccgcgttgtt
     2341 gatgtcgtcg cgcagcatgg tcgtgccctc ttggaaagcc ggaaaagctg atctaggcgg
     2401 ttcggggcgc ccagacaaca attctggttt cggaccgttc cgaaagcgga ggacccgttc
     2461 gacctccact atgcgggaaa cgcatgtgaa tccgcgcgtt cgtgctttga cgccggtctc
     2521 tggcccgact atgtaggggg ccatcatgac gacccccgaa aacgacgccg cctggccgga
     2581 ccacaagccg accgcgcttc tcgtgctcgc cgatggcacg gtgctgcagg gcttcggatt
     2641 tggcgccgag ggtcaggccg tgggcgaggt ctgcttcaac accgcaatga ccggttatga
     2701 ggagatcctc accgacccct cctatgccgg acagatcatc accttcacct tcccgcatat
     2761 cggcaatgtc ggcaccaacg aggaagac
//
LOCUS       DATLJT010001255         1934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_327575,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001255 DATLJT010000000
VERSION     DATLJT010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327575"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VM910_28820"
     CDS             complement(<1..804)
                     /locus_tag="VM910_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66549.1"
                     /translation="MSIALAGAQRDNVSESRFGFWRRSWAMVLKEFIQLRRDRVSFAM
                     IVMIPVMQLLLFGYAINTTPHNLPTAVLLQEDSDLGRSILKALENTAYFRFTREVHDV
                     QEFDNLLLSGKVLFGVEIPRGFERAVRRGDRPALLVAADATDPIAASSALSSLSVLVQ
                     TALAHDLFTGDPPSLPFEIRAHARYNPAAASRLNIVPGLVGTILTMTMLIFTALSVTR
                     EIERGTMESLLSMPIRPVEVMFGKIIPYVMVGFIQAAMILGIGVFLFGVP"
     gene            complement(804..1733)
                     /locus_tag="VM910_28825"
     CDS             complement(804..1733)
                     /locus_tag="VM910_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66550.1"
                     /translation="MNDASAIAIDVKGLSKSFVGREVVHDLSMQVKRGTIYGFLGPNG
                     SGKTTTIRMLCGLLTPDSGEGTCLGYDIRRDAGKIKLKVGYMTQRFSLYQDLSVRENL
                     EFVARLYGVGDPRGTAREMIRRLGLSGREEQLAGELSGGWKQRLALGACTLPNPELLL
                     LDEPTAGVDPKARRDFWNEIHALAGQGLTVLVSTHYMDEAERCHEIAYIAYGHLLAHG
                     TVEQVIAKSALTTYTVTGDDLQVLAERLAGQPGVDMVAPFGNSLHVSGRDKAALEATI
                     APWRDHPELHWQHSEPSLEDVFIELMSRSKDNF"
     gene            complement(1730..>1934)
                     /locus_tag="VM910_28830"
     CDS             complement(1730..>1934)
                     /locus_tag="VM910_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI66551.1"
                     /translation="NCAADLTAQIYFIATSAEYTPPVIYSLDERNKLVYLIQARPARP
                     DALRVGQPVSVYLNPKTPVADKR"
BASE COUNT          358 a          608 c          638 g          330 t
ORIGIN      
        1 cggcacgccg aacaggaaca cgccgattcc gagaatcatc gccgcctgga tgaagcccac
       61 catcacgtaa gggatgatct ttccgaacat cacctcgacc ggcctgatcg gcatcgacag
      121 caggctctcc atggtgccgc gctcgatctc gcgcgtgacc gacagcgccg tgaagatcag
      181 catggtcatg gtgaggatgg tgccgaccag accgggcacg atgttgagcc gggaagctgc
      241 cgccgggttg tagcgcgcat gcgcgcggat ctcgaacggt agcgacggcg gatcgccggt
      301 gaagagatcg tgcgcaagcg cggtctgcac caggacgctg agcgacgaca gcgcggacga
      361 ggccgcgatc ggatcggtgg cgtcggctgc aaccagcagc gccggccgat cgccgcgccg
      421 caccgctcgc tcgaagccgc gcggaatttc gacgccgaac aagaccttgc ccgacagcag
      481 gagattatcg aactcctgca cgtcatgcac ctcgcgggtg aagcggaaat aggcggtgtt
      541 ttccagcgcc ttcaggatgg agcgtccgag atcggagtct tcctgcagca gcacggcggt
      601 cggcaggttg tgcggtgtgg tgttgatggc gtagccgaac agcagaagct gcatcacagg
      661 aatcatcacg atcatggcaa acgacacgcg gtcgcggcgg agctggatga actccttcag
      721 caccatcgcc caggagcgcc gccaaaagcc gaagcgactt tcgctgacgt tgtcccgctg
      781 ggcaccggcc aatgcgatgc tcatcaaaaa ttgtcctttg agcgcgacat cagttcgatg
      841 aagacgtcct ccagcgacgg ctccgaatgc tgccagtgga gttccggatg gtcgcgccag
      901 ggcgcaatcg ttgcttcaag cgccgccttg tcgcggccgg agacgtgcag ggagttgccg
      961 aatggcgcca ccatgtccac gccgggctgt ccagcaagcc tctccgccag cacctgcagg
     1021 tcgtcgccgg tgaccgtgta ggtcgtcagc gccgatttcg caatcacctg ctcgacggtg
     1081 ccgtgcgcca gcaagtggcc ataggcgata taagcgatct cgtggcagcg ctcggcctcg
     1141 tccatgtagt gggttgagac cagtacggtc agcccctggc cggcgagcgc atggatctcg
     1201 ttccagaaat cgcgccgcgc cttgggatcg acgccggcgg tcggctcgtc cagcagcagc
     1261 agctccggat tgggcagcgt gcaggcaccg agcgcgagcc gctgtttcca gccgccggac
     1321 agttcgcccg cgagctgctc ctcgcggccg ctcaggccga ggcgcctgat catctcgcgc
     1381 gcggtgccgc gcgggtcgcc gacgccgtaa agccgcgcca caaattcgag attttcgcgc
     1441 accgacaggt cctgatagag cgaaaagcgc tgcgtcatgt agccgacctt gagcttgatc
     1501 ttgccggcgt cgcgacggat gtcgtaaccg aggcaggtgc cctcgccgct gtcgggcgtg
     1561 agcagtccgc agagcatgcg gatggtcgtg gtcttgccgg acccgttcgg gccgaggaag
     1621 ccgtagatcg tgccgcgctt cacctgcatc gacaggtcat gcacgacctc cctgcccacg
     1681 aacgatttcg acaggccctt gacgtcgatc gcaatggcag acgcgtcgtt catcgcttgt
     1741 ccgcaacggg tgtcttcggg ttgagataga cgctgaccgg ctggccgacg cgcagcgcgt
     1801 ccggccgcgc aggccgcgcc tggatcagat agacgagctt gttgcgctca tcgaggctgt
     1861 agatgaccgg cggggtatac tcggccgaag tcgcaatgaa atagatctgt gcggtgagat
     1921 cggcggcgca attg
//
LOCUS       DATLJT010001256         4916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_559166,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001256 DATLJT010000000
VERSION     DATLJT010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559166"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2262
                     /locus_tag="VM910_28835"
     CDS             <1..2262
                     /locus_tag="VM910_28835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HVI66552.1"
                     /translation="DLVEQRLDKLEAVLAQAIREPRDAAPLLAALLSIPTRERYPSLN
                     LSPQKQKEKTLKALLAQIEGLATRQPVMMLVEDAHWMDPTSRESFDIIIDRVPSLPVL
                     LIITYRPEFMPPWVGHPHVTLLSLNRLSPRQRAEMIGRVTGGKELPKEIVDQISDRTD
                     GVPLFIEELTKAILESGALRDAGDHYTIAGSLPSLAIPTSLHASLLARLDRLPPAREV
                     AQIGAALGRQFSHELISAVAPMQRQQLDEALSQLVSAELILRRGIPPDAEYTFKHALV
                     QDTAYSILLHSRRKQLHARIADVLEAQFPEVLETQPELLARHCTEGGRIEKAVDYRLR
                     SGKQAFARGAMAEAEAELRKGLDLLCSLPDGAALREQELNLQITFGNTLLATRGYAAA
                     EAGEAFEHARQLCQKLNRPQQLNRVLTGQFFNRLLRGELEQAEFLAGELRRLGEAASD
                     LSWKWNSLMRDGEVCCWLGKFIEARAHFEAALALWDPGFRDRISSPHDVYVNALIYLS
                     RALFCLGYLDQARLRRDDALAEARRLSPYTEVYTLRHAWYGDWAIEGVRSIEAMLRSA
                     ERISVISSEQGFPMFIGAGDIMRGWCLSSVGDAKTGISLLEEGLKKYAATGTKLVIPF
                     YLTVLAEAYGAAAQPKEGLNRLGDAAKLVETTQERWGEAEMYRVRGSLLLSLGERAAA
                     EESYCEALAVARHQSAKFWELRAGIDLGRLWRDQGKYIEARELLMPIYEWFTEGFDVP
                     VLRDAKALLAELR"
     gene            2745..3542
                     /locus_tag="VM910_28840"
     CDS             2745..3542
                     /locus_tag="VM910_28840"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66553.1"
                     /translation="MKIVASRDGTLIACEQGGRGPYLVLVHGTSSNSSRWEAVRPRLE
                     EHFTVARMDRRGRGGSGDSTEYAIEREFEDVATVVNALDAPILLFGHSFGAVCALEAA
                     MRTDKLAGLVLYEAPVCEGESLTSPDLLERLENLLSNGDREGVLRTFLAEVPRYSPKE
                     IEMLASSPAWPGRIAAAHTLPRELRSLDAYRLPEQVGKLTIPVLLLLGGDSPPIFGRV
                     NAVLEKTLPNSRSVVMPGQRHNAMDTAPDLLVGTVLDFWRNVALSAA"
     gene            3753..4757
                     /locus_tag="VM910_28845"
     CDS             3753..4757
                     /locus_tag="VM910_28845"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66554.1"
                     /translation="MASYIERRKFLATLGGAAVAWPLAVRAQQPERVGRIGVLMAYAQ
                     NDREYQSYLAAFREELQKLGWTEGRNVQIDYRWAALDPELMRRFARELIALQPDVILS
                     SSSPTTASLLQETRSIPIIFANIADPVGSGLVASLSRPGGNATGFMLFEGSLAGKWLE
                     LLKEIAPRVSRVAAVFNPATATFAEYYLNPVKAAAASFGVEVIAAPVHDTSELESVVA
                     TRAREPNSGLIVMPDAFLVAHREEVISLAARYRLPAIYFYRVFTEIGGLISYGNVSVD
                     NYRRSATYVDRVLHGAKPSELPVQAPVNFELVVNLKTAKALGLDVPPLLQQRADEVIE
                     "
BASE COUNT          994 a         1369 c         1512 g         1041 t
ORIGIN      
        1 gacttggtcg agcaacggct ggacaagctg gaagcggtcc tagcccaggc aatcagagag
       61 ccgcgtgatg ccgcgccgct gctcgcagca ctgctctcga ttccgacccg cgaacgttat
      121 ccgtcgctca atctgagccc acaaaagcaa aaggagaaaa cactcaaggc gttattggcg
      181 cagatcgagg ggctggcgac gcgacagccc gtgatgatgt tggtagagga cgcgcattgg
      241 atggacccta cttcgcgcga atcatttgat atcatcatag atcgtgtgcc ctccctacca
      301 gtactgctga taatcaccta tcggcccgag tttatgccgc cgtgggtcgg ccatcctcat
      361 gtgacgttgc ttagcctcaa tcgtctatcg cctcgacaac gggccgagat gattggacgc
      421 gtaacggggg gaaaggagtt acccaaggag atcgtcgatc aaatcagtga tcgaacggac
      481 ggagtgccgc tgtttatcga ggagttgacc aaagccattc ttgaaagcgg ggcgctgaga
      541 gatgccggtg accactacac aatagcgggc tcgttgccgt cgctggcaat cccaacgtcg
      601 ctccatgcat cgctactagc acggctcgat cgcctgccgc ccgcgcggga ggtggcacag
      661 atcggcgcag cgcttggccg gcaattctca catgagctga ttagcgccgt ggcgccgatg
      721 cagagacaac aactcgatga agccttatcg cagcttgtga gtgcggaatt gatcctccgg
      781 cgggggattc cacccgatgc agaatacaca ttcaagcacg ctctggtgca agataccgct
      841 tacagcattt tgctgcacag ccgacgtaag cagcttcatg cccgtattgc cgatgtactg
      901 gaagcgcaat ttccagaggt cttggagacc cagcccgaac tattggcaag gcactgcacg
      961 gaggggggcc ggattgagaa ggccgtcgac tatcggctaa ggtctggcaa acaagctttt
     1021 gctcggggag cgatggctga ggcggaagcg gaacttcgca aagggcttga tctgctttgc
     1081 agcttgcccg acggcgctgc cctccgggag caagaactca atctgcagat cacatttgga
     1141 aacacgctac tggcaacaag gggatatgcc gcagccgagg ccggggaggc attcgaacat
     1201 gcgcgtcagc tttgccagaa attaaaccgg ccgcaacaac taaaccgagt actgacaggt
     1261 cagtttttta accgcctgct gagaggagag cttgagcagg ctgaattcct agccggggag
     1321 ttacgtcggc tgggcgaggc agcgagtgac ttatcttgga aatggaacag cttaatgagg
     1381 gacggagaag tttgttgctg gcttggcaag ttcattgagg ctcgggccca cttcgaagct
     1441 gctcttgcct tatgggatcc cggttttcgt gatcggatta gctcgccgca cgacgtctat
     1501 gttaacgccc tgatttattt atctcgagct ctattctgcc tcggttatct cgaccaggcg
     1561 cgtctgcgga gggacgatgc attggccgaa gcgcggcggc tttccccgta taccgaggtt
     1621 tatacgctac gccatgcttg gtacggcgac tgggcaatcg agggcgtgag atcaatcgag
     1681 gcgatgctgc ggtcggcgga gcgcatttcc gttatctcga gcgagcaggg tttccctatg
     1741 tttatcgggg ccggcgacat catgcgcggc tggtgtttga gctcagttgg ggatgcaaag
     1801 acgggcatat cgttgctcga agaggggcta aaaaagtatg ccgcaacggg tactaaattg
     1861 gtgattccat tttatctcac ggtgcttgca gaggcttatg gtgcagccgc gcagcctaag
     1921 gagggattaa accgactcgg cgacgcggcg aagttggtgg aaacgacaca ggaacgttgg
     1981 ggagaggccg agatgtatcg ggtgcggggg agcttattgc tctccctcgg tgagcgggct
     2041 gcagcggagg aaagttattg cgaggcgctc gcggtggcgc gacatcagag cgcgaagttt
     2101 tgggagctgc gcgcgggcat cgacttgggg cgactttggc gcgaccaagg caaatacatc
     2161 gaggcgcgtg agctcctcat gccaatctat gaatggttta ccgaaggctt cgacgtgccg
     2221 gtcttgcgag atgcaaaggc gctgcttgcc gagctgcgat gatttaataa agcccattcg
     2281 tgatcaagtt cgcggtggct agggcacccg gcctcgagag ccgcccggag ctgttcgccc
     2341 gcgccggaga ggcgatcgaa tactttcttt gctgcatctg ctccggtctg gaagtggcac
     2401 taagcggaca tgcccgtcgt gtccgtcgat gtccgctttc gggcaaaccg gacattgagc
     2461 cgacatcgcc gaatgaccga gtctgagacc cgaagcggac actgggtctc ccttaacgag
     2521 cgtagtttga accgctacga tgccctgtcc tgagccttgg gcgcggccat gccacgtcgc
     2581 gaattcatcc tattccttag tggggcgtcg acgtcgccgc tggtagcgcg agcccaagcg
     2641 catgcggcgc atcggcgtgc aatctctaca gggttgagcg atggcattgc tgcaacaaga
     2701 cctagccatg agtcggtctg ttgctcgctt ccgggagggt tacgatgaag atcgtggcga
     2761 gcagggacgg cacgttaatt gcgtgtgagc aaggtggtcg cggaccgtat ctcgtgctcg
     2821 ttcatgggac ctcttcgaac agctcgcggt gggaagcggt ccgtccccgt ctcgaggagc
     2881 atttcactgt cgctaggatg gaccgccgtg gtcgcggcgg cagtggcgac agcacggaat
     2941 atgcaattga gcgtgagttt gaagacgttg ccacggtcgt caacgccctc gacgcgccca
     3001 tactcctttt cggccactcc ttcggggctg tctgcgcgtt ggaggcggcg atgcgaacgg
     3061 ataagctcgc tggtcttgtg ctctatgaag cccccgtttg cgagggcgag agcctgacct
     3121 ctcccgactt actagagcgt ctggagaacc ttctgtccaa cggtgatcgc gaaggcgtgc
     3181 tcagaacatt cctcgccgaa gtgccgaggt attcacccaa agaaatcgaa atgcttgcgt
     3241 cctctccggc ttggcccggc cgcattgccg cggcacacac actgccgcga gagcttaggt
     3301 cattagatgc ctatcgcctg cccgaacagg ttggcaagct gacaatacca gtcctgcttc
     3361 ttctcggcgg cgacagcccg cccatcttcg gcagagtaaa cgccgtgttg gagaagaccc
     3421 tgccgaactc ccgaagtgtg gtgatgccgg gccagaggca taatgcgatg gacactgccc
     3481 ccgatctact cgtcggcaca gtgctcgatt tttggcgaaa cgtggcccta agcgccgctt
     3541 aacatgtccg ccatagcaag atcgcgggtg gacttccgct tttgatggaa gagcggtcgt
     3601 gcagcgacat cgcggcggtg accgagtttg atccctcaag cagccttcgg gatcatccac
     3661 gcgacttccc tatcgccctc agtgactgac cgcgcgatgg aagcgtggta tcatctgccc
     3721 attgcgtgaa tgacccccaa ccggagggtc acatggcaag ctacatcgaa cgacgaaaat
     3781 ttttagccac gctcggcggt gcggcggtgg cgtggccact cgcggtgcgc gcgcagcagc
     3841 ccgagcgcgt ggggcgcatc ggcgtcctaa tggcatatgc ccagaatgac cgggaatatc
     3901 agagctatct cgcggcattt cgggaggagc tccagaagct cggctggact gagggccgta
     3961 acgtccagat cgactatcgc tgggcagcac tcgatccaga gttaatgcga cgattcgcca
     4021 gggaactgat cgcactgcag cccgacgtta tcctctcgtc gagctcgccg accactgcat
     4081 cgctgctgca agaaacgcgc agcattccca tcattttcgc taacatcgcc gatccggttg
     4141 gcagcggcct tgtcgcaagt ctgtcgcgac cgggcggcaa cgctacgggt ttcatgcttt
     4201 ttgaaggatc gctagctggt aagtggctgg agctgctgaa ggagattgcg ccgcgcgtct
     4261 ccagggttgc cgccgtgttc aatccggcaa cggcgacatt tgccgaatat tacttaaacc
     4321 ccgtgaaagc cgccgctgcg tccttcggag tggaggtgat cgcggcgccc gttcacgaca
     4381 cgtccgagct cgaatccgtc gttgccacgc gggctcgcga gccgaatagc ggcttgatag
     4441 tgatgccgga tgcgttcttg gtcgcccatc gcgaggaagt catatcgctg gctgccaggt
     4501 accgtctccc tgccatctat ttttatcggg tcttcaccga gattggtggt ctaatttcat
     4561 atgggaacgt ttcggtcgat aattatcggc gctcagcgac ttatgtcgac cgtgtcctac
     4621 atggcgccaa gccgagcgag cttcccgtgc aggctccggt caattttgag cttgtcgtca
     4681 acctcaagac agccaaggca ctcggtcttg acgtgcctcc gttgcttcag cagcgcgccg
     4741 acgaggtgat cgaatgatgt gaagggaggt tcgacatgaa acttccccgc cgaaattttc
     4801 tgtatctggc ggcgggtgct gcggcactgc ccgccgtctc acgcgtcgca agggcgcatc
     4861 cggtccgatg tgctggccgt gcaaatgcgc ttgattaatt cagatcgcag taagat
//
LOCUS       DATLJT010001257         2439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3314664,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001257 DATLJT010000000
VERSION     DATLJT010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3314664"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1683)
                     /gene="zwf"
                     /locus_tag="VM910_28850"
     CDS             complement(73..1683)
                     /gene="zwf"
                     /locus_tag="VM910_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVI66555.1"
                     /translation="MLPAREQEPATKSASEETMMLEASGRQAQIENAAVAPPCAMVIF
                     GAAGDLTKRLVVPALYNLTTTRQLSDGFQLVGVDRAAKSTQEWRAGLTDTMKEFVGHE
                     GKFQVERIDQNAWQWLADRMSYLQGDLNDPETYRRLHEHLAELDKTAGTAGNYLFYLA
                     VADRFFSLAVAGLGAADLVTETQGRWRRVVIEKPFGHDLPSAKALNAEILKTLQENQI
                     YRIDHFLGKETVQNIMALRFANGLFEPLWSRQHIDHVQITAAETVGVEHRGKFYEKTG
                     ALRDMVPNHVFQLLAMTAMEPPISFDADAVRSKKAEVIQAMHPIDPAHAVTTAVRGQY
                     DAGTVLGKQVRAYRQEPDVAPDSTTETFVACKLKIDNWRWAGVPFYLRTGKYLKRRST
                     EIAIRFRQAPYTLFRGTCVERMNPNWMILRIQPDEGIAFEFAAKLPGPSVKLSNVSMD
                     FAYKTYFKMAPNTGYETLLYDCMIGDATLFQRADNIEAGWQAVQPILDAWANNSPRDF
                     PNYAAGGTGPVAADELLTRDGRAWRPLD"
     gene            complement(1683..>2439)
                     /locus_tag="VM910_28855"
     CDS             complement(1683..>2439)
                     /locus_tag="VM910_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVI66556.1"
                     /translation="IVNGLSLSKLRAFGSTFFIFSDYARPAIRLSALMELPTIFVFTH
                     DAIGDGEDGPTHQPVEQLISLRAVPGLVVLRPADANEVVEAYRYIMQLRNQPAVLALS
                     RQALPTFDRRKYASAAGVARGAYVMADPADNSPEIILIASGSEVALIVQAHETLAAQG
                     IRSRVVSMPSWDIFEQQPQAYRDDVLLPTVKARLAVEQGSVLGWDHYVGPAGRVTGMK
                     TFGASAPLKELDRKFGFEPERVVATAMEVLGRA"
BASE COUNT          486 a          741 c          714 g          498 t
ORIGIN      
        1 gagggtgcca tccacgtccg ccagcaccag cgaaatcttg cgcttctctg ccattggctt
       61 ctccggcacc gctcagtcga gcggccgcca ggcgcgacca tcgcgcgtca gtagttcgtc
      121 cgctgccaca gggcccgtcc cacccgcggc ataattcgga aaatcccgcg gggaattgtt
      181 cgcccaggcg tcgaggatgg gctgcaccgc ctgccatcca gcctcgatat tgtcggcgcg
      241 ctgaaacaat gtcgcgtccc cgatcatgca gtcgtagagc agcgtctcat atccggtgtt
      301 gggcgccatc ttgaaataag tcttataggc gaagtccatg ctgacattac tcagcttcac
      361 cgacggtccg ggcagcttag ccgcgaactc gaacgcgatt ccttcgtcgg gctggatgcg
      421 aagaatcatc caattgggat tcatccgctc tacgcacgtc ccgcgaaaga gcgtatatgg
      481 ggcctgacga aaacggatgg cgatctcagt tgatcgtcgc tttaaatatt tgccggtgcg
      541 cagatagaac ggcacgccag cccagcgcca attgtcgatc ttcagcttgc aggcaacgaa
      601 agtctcggtc gtcgaatccg gcgccacatc aggttcctgg cgataagctc ggacctgctt
      661 gcccaacacg gtgccagcat cgtactggcc acgcaccgca gtggtcaccg catgcgccgg
      721 gtcgatcgga tgcatggcct ggatgacttc ggccttcttg ctacgaacgg catcggcatc
      781 gaaggagatc ggcggctcca ttgccgtcat ggccagaagc tggaacacgt ggtttggcac
      841 catgtcgcgc agcgcaccgg tcttttcgta gaacttgccg cggtgctcca ccccaacggt
      901 ttccgccgcg gtgatctgga catggtcaat gtgctgtcgg ctccatagcg gctcgaataa
      961 gccgttggcg aagcgtagcg ccatgatgtt ctgcaccgtt tccttgccga ggaaatggtc
     1021 gatccggtag atctgatttt cctgcagagt ttttagaatc tcggcattga gcgctttggc
     1081 cgaaggtaaa tcgtggccaa acggcttttc gatcacgacc cggcgccatc ggccttgtgt
     1141 ctccgttaca aggtccgcag caccaagtcc ggcgactgcc aagctaaaaa agcgatctgc
     1201 cacggcgaga tagaaaagat agttacctgc tgtgcctgct gtcttatcca gctctgccag
     1261 atgctcgtga agcctgcgat aggtctccgg atcgttgagg tcaccctgca gatagctcat
     1321 gcgatcggca agccactgcc acgcgttctg gtcaatacgc tcgacctgga atttgccctc
     1381 gtggccgacg aattctttca tcgtgtcggt gaggccagca cgccattctt gcgtgctctt
     1441 ggcggcccga tcgacgccga cgagctggaa gccgtcggac aactgccggg tggttgttag
     1501 attataaagt gccgggacca cgagacgctt cgttaggtca ccggctgcgc cgaaaatcac
     1561 catcgcgcag ggtggtgcta ccgcggcgtt ttcaatttgt gcttgcctgc ctgacgcctc
     1621 gagcatcatc gtctcctcgc tggctgactt cgttgcgggc tcttgctcgc gcgcaggcag
     1681 catcaggcgc gtccaagtac ctccatagca gtcgccacga cccgttcggg ctcgaaacca
     1741 aacttgcgat ccaattcctt gagcggcgcc gaggcgccga aggttttcat gccggtgaca
     1801 cggccagcag gaccgacata atggtcccag ccaagcaccg atccttgctc gacagcgagg
     1861 cgcgctttca ccgtgggcag caaaacgtca tcccgatagg cctgcggttg ctgttcgaaa
     1921 atgtcccagg acggcatcga gacaacacgc gatcggattc cttgagccgc cagcgtttca
     1981 tgggcctgga cgatcaacgc tacctcgctc cccgaagcga tgaggatgat ctcgggagag
     2041 ttatcagcag gatcggccat gacataggcg ccgcgtgcca cgccggccgc cgatgcatat
     2101 ttgcgacgat cgaatgtcgg tagcgcctgg cgcgaaagcg cgaggaccgc cggttggttg
     2161 cgcaactgca tgatgtagcg gtacgcttcg acgacctcat ttgcatccgc cggccggagc
     2221 acgaccaggc cgggaacggc gcgcagcgag atcaactgct caaccggttg atgggtcggt
     2281 ccatcttcgc catcgccgat ggcatcgtgg gtgaaaacga aaatcgtcgg caattccatg
     2341 agcgccgaga gccgaatggc cggccgggcg tagtcgctga agatgaagaa cgtcgatcca
     2401 aaggcccgca gcttcgagag cgacagaccg ttgacgatc
//
LOCUS       DATLJT010001258         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1936900,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001258 DATLJT010000000
VERSION     DATLJT010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936900"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VM910_28860"
     CDS             complement(<1..235)
                     /locus_tag="VM910_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HVI66557.1"
                     /translation="MGLSEPALVTQREKIIDPRKGKAVGGDDPFFLQVKSELSDKGFL
                     IAAADDLIAWARTGSLMWMTFGLACCAIEMMQVS"
     gene            complement(226..591)
                     /locus_tag="VM910_28865"
     CDS             complement(226..591)
                     /locus_tag="VM910_28865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVI66558.1"
                     /translation="MAQLLLAYLPLIVFIGVAAVIGLALLVVAFFVAYKRPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVSFLFPWAAAFGQLGVYGFWSMMVFL
                     AVLTVGFIYEWKKGALEWD"
     gene            939..1877
                     /locus_tag="VM910_28870"
     CDS             939..1877
                     /locus_tag="VM910_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI66559.1"
                     /translation="MNDASRAASQAAVIRGEEHWTSKDGTVKLFMFEKCGDDPNSAAG
                     TVLFVHGSSMASQPTFDLQVDGRPDSSVMDWFARRGFDCWCVDMEGYGRSTKDRDNNA
                     PIAQGADDCFAAATYIQQLRGKRPLLVYGISSGALRAALFAQRHPQLVARLALDAMVW
                     TGAGSPTLAERKKKLPEFKAKSRRPIDKAFIHSIFDRDHPGTAEEMVIDAFADAVTAL
                     DSSVPTGTYVDMCSKLPVVDPAKITVPTLIMRGQWDGIAGFEDLLAFFARLPNPDKHF
                     AVMPGISHASFQQKNYALVYHILWSFFTQPAPIYRG"
     gene            complement(2070..2222)
                     /locus_tag="VM910_28875"
     CDS             complement(2070..2222)
                     /locus_tag="VM910_28875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66560.1"
                     /translation="MRVLLPLYRSGEMPGGSPLKVRPGSGVQAAGIGWQPTTVQAMRA
                     GGCAVV"
     gene            complement(2246..2322)
                     /locus_tag="VM910_28880"
     tRNA            complement(2246..2322)
                     /locus_tag="VM910_28880"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2286..2288),aa:Asp,seq:gtc)
BASE COUNT          512 a          757 c          699 g          417 t
ORIGIN      
        1 tcgacacctg catcatctcg atcgcgcagc aggcaagccc gaacgtcatc cacatcaacg
       61 agccggtgcg cgcccaagcg atcagatcgt cggcagcggc gataagaaaa cccttgtcgg
      121 aaagctcgct cttgacctgc aagaaaaacg ggtcgtcgcc gccaacggcc ttgcccttcc
      181 gcggatcgat aattttctct cgctgagtga cgagtgcagg ttcgctcaat cccattcgag
      241 cgctcccttt ttccattcgt aaatgaaccc gacggtgagc accgccagga agaccatcat
      301 cgaccagaag ccgtacacgc cgagttggcc gaaggccgcc gcccacggaa acaggaagct
      361 gacctcaaga tcgaaaatga tgaacaggat cgcgaccagg tagaaacgta cgtcgaattt
      421 catgcgcgcg tcgtcgaacg cgttaaagcc gcactcgtag gcggagagtt tttccggatc
      481 gggccgcttg taggccacga agaaggcgac gacgaggagc gcgaggccaa tcaccgccgc
      541 aacgccgata aaaacaatga gcggcagata agcgagcagc aattgagcca gagattgact
      601 catttccaac ccttctggcg ggcaaacaag gacaccctcc atataggaaa ggggcccggc
      661 gcggcgctat cccgcgtgca acggacgtgc gccgatttgc ctcaaaatca agcggattcg
      721 cgctgcaggc cggatgtcgc gacccttagc gcagtgcaca aaaaggtgca aggcaggccg
      781 gcgcatagcg acccgcgacg cgctcggcga taggggctgt cgcgtcgcat tctgccaata
      841 cgcatgttag gttgcgcgat aacctccagt cgtctcgctc gggttgcgat tcctgctcgg
      901 cagggatggc aatagcgaca atctccagca ggtgaacgat gaacgatgca agtcgggctg
      961 caagtcaggc cgcggtaatc cgcggcgaag agcattggac cagcaaagat ggcactgtga
     1021 agctgttcat gttcgaaaaa tgcggcgacg atccgaacag cgccgccggc accgtcctct
     1081 tcgtgcacgg ttcttcgatg gcgtcacagc cgaccttcga tttgcaggtc gacggccggc
     1141 ccgattcctc agtcatggac tggttcgccc gccgcggctt cgactgctgg tgcgtggaca
     1201 tggaaggcta tgggcgctcg accaaggatc gcgacaacaa tgctccgatc gcgcaaggcg
     1261 ccgacgactg cttcgccgca gcgacttata ttcagcagct ccgcggcaag cggccattgc
     1321 tcgtctacgg catctcgtcg ggcgcgctgc gcgcggcttt attcgcgcag cgccacccgc
     1381 agctcgttgc ccggctcgcg ctcgacgcca tggtgtggac cggtgcgggc tcgccgacac
     1441 ttgccgagcg gaaaaagaaa cttcccgaat tcaaagccaa gagccggcgc ccgatcgaca
     1501 aggcattcat tcattcgatc ttcgatcgcg atcatcccgg caccgcggaa gaaatggtca
     1561 tcgacgcatt cgccgatgcc gtcaccgcgc tcgacagctc agtgccaacc ggcacctatg
     1621 tcgatatgtg cagcaaactc ccggtggtgg accctgcgaa aattaccgtg ccgacgctga
     1681 tcatgcgcgg ccagtgggac ggcatagccg gcttcgaaga tctgctcgcc ttcttcgccc
     1741 ggttgccgaa tcccgataag catttcgcgg tgatgccggg catttcgcac gcgagcttcc
     1801 agcagaagaa ttacgcgctg gtctatcaca tcctgtggag cttcttcacc cagccggccc
     1861 cgatttatcg ggggtgatgc agtcaacaga tgctgaaact ttgccggtgc ggtcccgttg
     1921 gtccaggcac gaggtcgtta aaacgagcgc gcagcaagta tgagccctgt cgccgcaatg
     1981 gtcgcagccc tcctgaccgc aggattgact acgccggctt cgtccgccaa tgcggccagt
     2041 agccagcacc gcagccaccc gtcccagcat cacaccacgg cgcagccgcc ggcgcgcatt
     2101 gcctgcacgg ttgtgggctg ccagccaatc ccggccgcct gcacgccgct accagggcga
     2161 accttgagcg ggctgccacc aggcatctcg ccgctgcgat agagcggcag cagaactcgc
     2221 aaaaaatctt gtgttttcaa tagaatggcg ggagcgacgg gactcgaacc cgcgaccttc
     2281 ggcgtgacag gccgacgctc taaccaactg agctacgccc ccgcgggcgc cgtcggcagt
     2341 taaggctggc ccttcgccaa gtcaaggatt tagggcctaa acgcg
//
LOCUS       DATLJT010001259         6553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3545458,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001259 DATLJT010000000
VERSION     DATLJT010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545458"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VM910_28885"
     CDS             <1..842
                     /locus_tag="VM910_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVI66561.1"
                     /translation="TLVSFFHGSNDGQKGMGLIMLILIGTVPTAYALNRTMPASQIEA
                     FIRNSEAASKVIEAKAGGHQIIGNPRPAVTLYVSQREINEGTFPSLAVLVREIAQQVS
                     QYGSLAKLPAEAVGNTRNDMYLASEALRFLMKDKEADLTSAEIATLNSYKGSLDNATK
                     FIPTWVKIAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGACAEITAAATIAAAD
                     GYGLPVSTTHVLSSGIAGTMAANGSGLQWSTVRNIAMAWVMTLPIAMLLSGFLYFIFS
                     QIF"
     gene            complement(992..1888)
                     /locus_tag="VM910_28890"
     CDS             complement(992..1888)
                     /locus_tag="VM910_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66562.1"
                     /translation="MPLKKLGIIEIPNAANSEFDHAAFDPKTRRVFIAHTARDCIEVI
                     DHDAQRHIATLPGFGGVAGAVADDGEVLATNRGSASVAWLDAATFKTRAVFQCGPRPN
                     GAAIVKRSGVGIAACIGDDRNTPTLHLFGLKDGSHRAIDLPGRPRWCVTDGAAERLFL
                     CIREPSMVLVARLPELSDIVHWRLPSGGAHGLDIDHARHRLYAACDERALLEIDSGSG
                     KVTNIWPLAGAPDVTFFNPATGLVHVAIGEPGLVETIDPRSGASERTATGAGAHTTAI
                     VVPDRLYVISPSHGGVLVLADT"
     gene            complement(2091..2783)
                     /locus_tag="VM910_28895"
     CDS             complement(2091..2783)
                     /locus_tag="VM910_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI66563.1"
                     /translation="MPDPIVYGFPRSTFVNIARLILTHKDVAYTFHDLEEVMGKAEHL
                     ALHPFNRVPIFKHGDFTVYETSAIASYIDEAFAGPRLTPQNVRARARMNQWISAVNSY
                     YYPYMIYHVTHERLVFPELGIASDEKVVAHALPKVELALGVMERELSHGQDYLLGSEL
                     TLADFFILPSTFAFSLTEEARAMYPKFPAFCRWRERMEILPATKKLRAILPPRDPIRH
                     AREWANSHRPKY"
     gene            complement(2826..3050)
                     /locus_tag="VM910_28900"
     CDS             complement(2826..3050)
                     /locus_tag="VM910_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66564.1"
                     /translation="MPTLTLQPARPAARLNQLIWGALLAARWRALRRQWARHRQREAL
                     RAIADDPHLLRDLGLTRRQALVEAEKPFWR"
     gene            3169..4107
                     /locus_tag="VM910_28905"
     CDS             3169..4107
                     /locus_tag="VM910_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HVI66565.1"
                     /translation="MRPRLPPLNALKAFEAAARHESFTRAAEELCVTQGAVSHQVKAL
                     EAELAIKLFNRERQRLIITEAGRDYLAVVRDALDWIAVGTERLLQRQNAGVLTVSTSP
                     DFAAKWLVHRLGHFAEAHSTIDLRVSATMHHVDFAREDVDMAVRHGDGNWPGLDAVEL
                     SAEQLFAVCSPKLLSGRRLGKPADILKFPLLHLDSRADWRKWLQAVGISDANVKHGPV
                     LNRASMVIDAAINGQGIALARTTLAAWDLLNGRLVRPFPESLRLSKTYWIVCPKATSK
                     VPKIVTFRGWLLAEASQDLRRLKKRGWSLKLVEADR"
     gene            complement(4387..5424)
                     /locus_tag="VM910_28910"
     CDS             complement(4387..5424)
                     /locus_tag="VM910_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cj0069 family protein"
                     /protein_id="HVI66566.1"
                     /translation="MFHPTARLGRIAILWRGDEAARRSASPETSRFKAVFAALADVGV
                     DAEPVVYEDDVVDTVRAQLATFDGVLVWVNPIHEGRNRANLDALLREVARRGAWVSAH
                     PDVILKMGTKEVLHRTRMMGWGCDTALYRTVEAMRVELPSRLAAGPRVIKRNRGNGGQ
                     GVWKVEPLASPRNRPMVRLLDATKDASEEVELNEFLERCSEYFEDGCVIDQPFQARLS
                     EGVVRCYMAGDRCAGFGYHKVKALVAAPAARAEAGPRLYTFNADPRFQRLRRLMEDEW
                     TPQLISLLEIPRSDLPIIWDADFMLGPRSADGTDSYVLGEINVSSVFPIPDEASAEIA
                     RRVADRLKSKL"
     gene            complement(5899..>6553)
                     /locus_tag="VM910_28915"
     CDS             complement(5899..>6553)
                     /locus_tag="VM910_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66567.1"
                     /translation="YVLTFLFASVSPLWTAVAAIIMVLFASRETVARQKRRLRGFWTY
                     GLGAGCTLLAAGTVTTFSLLTQLRPEPWYHPRYALPLLGMILGNTMTGVSLGLDVLTN
                     GLVRERAAVEACLALGGTRYQALLPIIRDALRSGFMPTINGMAAIGLVSLPGMMTGQI
                     LAGVEPTDAVKYQLLIMFLIAGGTGLGTLAAVIGGGHLLTDHRHRLRLDRIAGDAAE"
BASE COUNT         1281 a         2057 c         1997 g         1218 t
ORIGIN      
        1 gcaccctggt ctcgttcttc cacggttcga atgacggcca gaagggcatg ggcctcatca
       61 tgctcatcct gatcggcacc gtgccgaccg cctacgcgct caaccgcacc atgccggcga
      121 gccagatcga ggcgttcatc aggaattcgg aagccgcgag caaggtgatc gaggccaagg
      181 ccggcggcca ccagatcatc ggcaatccgc ggcccgccgt gacgctttac gtgtcgcagc
      241 gcgagatcaa cgaaggaacc ttcccgtcgc tggccgtgct ggtgcgcgag atcgcccagc
      301 aagtcagcca atacggctcg ctcgcgaaac tcccggcgga agccgtcggc aacacccgca
      361 acgacatgta tctcgcctcc gaagctcttc gctttctgat gaaggacaag gaggccgacc
      421 tgacgagcgc ggagatcgca acgctcaaca gctataaggg ctcgctcgac aacgcgacca
      481 aattcattcc gacctgggtg aagatcgcgg tcgcgatcgc gctcgggctc ggtaccatgg
      541 ttggctggaa gcgcatcgtc gtcaccgtcg gcgaaaagat cggcaagaca cacctgacct
      601 acgcgcaggg ggcgtgcgcc gagatcacgg cggcggcgac cattgcggcc gccgacggct
      661 atgggctgcc ggtctcgaca acccacgtgc tgtcgtcagg tatcgccggc accatggcag
      721 ccaacggatc gggcttgcaa tggtcgacgg tgcgcaacat cgcgatggcc tgggtgatga
      781 ccttgccgat cgcgatgctg ctgtccggct tcctgtattt catcttctcc cagatcttct
      841 agctcactcg caaaccgcgc ggcgcagcag tgcaaaaggc ccgctaccga gtgtagcggg
      901 ccttttcgtt gcgagaggtc tttagggtca cctcggtcgc aggccgccct cgcctcctaa
      961 tataatcctg cttttgagcc tttcttgtca ctcacgtgtc agccaggacg agaactccgc
     1021 catggctcgg cgaaatcacg tacagccggt ccggaacgac gatcgcggtc gtgtgcgcgc
     1081 ctgcgcccgt cgcggtccgc tcgcttgcac cactccgcgg atcgatcgtt tcgacaagcc
     1141 cgggctcgcc aatcgccacg tgaacgaggc cggttgccgg gttgaagaat gtcacgtcag
     1201 gcgcgcccgc aagcggccag atgttcgtga ccttgcccga gccagagtcg atctcaagga
     1261 gcgcgcgctc atcgcaagcg gcatagagac gatgccgggc atggtcgatg tcgaggccat
     1321 gggcgccccc tgacggaagc ctccaatgaa cgatatcgct cagctccggc aagcgcgcca
     1381 ccagcaccat cgacggctcg cggatgcaga gaaacagccg ttccgcagcc ccatccgtca
     1441 cacaccagcg cggtcggccg ggtagatcaa tcgcgcgatg gctgccgtcc ttgaggccga
     1501 acagatgcag cgtaggcgtg ttgcgatcgt cgccgatgca ggcggctatt ccgacaccgc
     1561 tgcgcttgac gatcgccgca ccattcggcc gcggaccgca ttggaacacg gcccgggttt
     1621 tgaacgtcgc agcgtccagc cacgcaacgc ttgccgagcc gcggttggtg gcaaggactt
     1681 ctccgtcgtc ggcgacggcg ccggccactc ccccaaatcc cggcaatgtc gcgatgtgcc
     1741 gttgggcatc gtggtcgatc acctcgatgc aatcgcgcgc ggtgtgggct atgaagaccc
     1801 gccgggtctt cgggtcaaac gccgcgtggt caaactcgct gtttgcggcg ttgggtatct
     1861 cgatgatgcc aagtttcttc agcggcatga cggccttccg gcgcgttgcc accacgaccg
     1921 cttacgaagc gaagcacgtc gaatgggagg accgtccatg cgcaatctcc tcgaagttcg
     1981 agtgttgcgc agagcttgga cgggaagccg tctcgcgggg agtctgcgtg tccccgtgtt
     2041 ttgaccctag caacgccgag cttgatcggc aagttgccat tcttgacgcg tcaatatttc
     2101 ggcctgtgcg agttggccca ttcgcgcgcg tgccggatcg ggtcacgggg cggcaggatc
     2161 gctcgcaatt tttttgtcgc cggcaggatt tccatccgct ctcgccatcg gcagaacgca
     2221 ggaaatttcg gatacatcgc cctggcttct tcggtcaggc tgaaggcaaa ggtgctcggc
     2281 agaatgaaga agtcggcaag cgtcagctcg gagccgagca gatagtcttg tccatgcgac
     2341 agctcgcgtt ccatcacgcc gagcgccagt tcgaccttgg gcagcgcatg cgccactact
     2401 ttctcatcag acgcgatgcc gagctcggga aacaccagcc gctcatgcgt gacatgatag
     2461 atcatgtagg ggtagtagta ggaattgact gcgctgatcc attggttcat tcgcgcgcga
     2521 gcgcgcacat tctgcggcgt cagccgcggt ccagcgaacg cctcgtcgat gtaagaagcg
     2581 atggcgctgg tctcatagac agtgaaatca ccatgcttga aaatcggcac acggttgaac
     2641 ggatgcagtg cgagatgctc cgccttgccc atcacctcct ctagatcatg gaaggtgtag
     2701 gccacatcct tatgggtcag gatcagcctc gcgatgttga caaaggtgct tcgcgggaaa
     2761 ccgtagacga tcggatcggg cattgtcgtt ctctccttgg aatcgcagca agtgccttgt
     2821 gcgcatcacc gccaaaatgg tttttcggct tcgaccagcg cctgccgccg ggtgaggccg
     2881 agatctctga gcaggtgcgg atcatcggcg attgcacgca gcgcctcgcg ctgcctgtgc
     2941 cgcgcccact gccgtcgcag cgcgcgccat cgggccgcaa gcagggctcc ccaaattaat
     3001 tgattaagtc gagcggccgg tcgcgccggc tgaagggtca gggttggcat cgacaagctc
     3061 caaaactcat gaaatgctgc gtctgagcga ttcattaggt ccgccggagg agtacccgca
     3121 aagcatgctt tctcttgctt gccccaattt ttctcatgaa gtcgtgccat gcgaccgcgc
     3181 ctgccgccgc tcaatgccct gaaggccttc gaggctgccg cccggcacga aagcttcacg
     3241 cgtgcggcgg aggagctttg cgtcacgcag ggcgcggtaa gtcatcaggt caaagcgctg
     3301 gaagccgagc ttgcgatcaa gcttttcaac cgcgagcgcc agcggctgat catcacggaa
     3361 gccggacgcg actacctcgc ggtcgtgcgc gacgcgctgg actggattgc cgttggcacc
     3421 gaacggctgc tgcagcggca gaatgccggc gtcctcacgg tcagcacctc gcctgatttc
     3481 gccgccaaat ggctggtgca ccggctcggc cattttgccg aggcgcattc caccatcgac
     3541 ctcagggttt cggcgacgat gcatcatgtc gactttgcgc gcgaggacgt tgacatggcg
     3601 gtgcgtcacg gtgacggtaa ctggccggga cttgacgccg tggaactgag tgccgaacag
     3661 ctttttgccg tctgcagccc gaagctcttg tcgggtcgcc ggctaggcaa gccggcagac
     3721 atcttgaagt ttccactgct ccatctcgat agccgggcgg attggcggaa gtggctgcaa
     3781 gcggtcggca tcagcgacgc caatgtcaag cacggaccag ttctcaaccg cgcgagcatg
     3841 gtgatcgacg ctgcaatcaa cggccagggg atcgcattgg cgcgcacgac actcgcagca
     3901 tgggacctgc tcaatgggcg gttggtgcgg ccgttcccgg aatcgctgcg gctgtcgaag
     3961 acctactgga tcgtctgtcc caaagcgacg tcgaaggtgc cgaagatcgt gaccttccgc
     4021 ggctggttgc tcgcggaagc ctcgcaggat ctgcgtcggc tgaagaagcg cggttggtcg
     4081 ctgaaactcg tcgaagcgga ccggtgacgc actgcggtat gcaaaacata tcgcgcgtca
     4141 gtccaccatc caatccggct gcaggatcgg atacgcaatc agaccgcgag agcgctcaac
     4201 ctccgcaagg cggatcgaag gctgcagggc accagtcact ggctgcgtga ttcgatcctg
     4261 cagatcaaac aattcgtcta cagtaccatc atagcgcgag ctccggacgt gcacgcctcc
     4321 ctcggtttcg gcgaacaggc atcagcgggc gggcgccgag actatgacgc gcaaaccgtc
     4381 gaccgctcag agcttcgatt tcagccgatc ggccactcgg cgggcgatct ccgccgacgc
     4441 ctcgtcgggg atcgggaaga ctgaactgac gttgatctcg ccgagcacgt aactgtcagt
     4501 cccgtcggcg ctgcgcgggc caagcataaa gtctgcatcc cagattatcg ggagatcgct
     4561 cctcggaatc tccagcaagg agatcagctg cggcgtccac tcgtcttcca tcaaccgacg
     4621 caagcgctgg aagcgcggat cggcattgaa agtgtagagc cgcggtccgg cttcggcccg
     4681 cgccgccggt gccgcgacca gtgctttgac cttgtggtag ccgaagccgg cgcagcgatc
     4741 gcctgccatg tagcaacgca ccacaccttc gctcaggcgc gcctgaaacg gctgatcgat
     4801 cacgcagccg tcttcgaaat actcgctgca ccgctcgaga aactcgttga gctccacctc
     4861 ctccgatgca tccttcgtgg catcaagcaa gcgtaccatc ggccggttgc gtggacttgc
     4921 cagcggctcg accttccaca cgccctgacc gccgttgccg cggttgcgct tgattacgcg
     4981 agggccagcg gcgagccgtg acggcaattc cactcgcatc gcctcgaccg ttcgatagag
     5041 ggctgtgtcg cagccccacc ccatcatgcg ggtgcgatgg agcacctcct tggtgcccat
     5101 tttgaggatc acatcgggat gggcgctcac ccacgcgccg cgcctcgcga cctcgcgcag
     5161 caacgcatcg agattggcgc gatttcggcc ctcgtgaatc gggttcaccc atacgagcac
     5221 gccatcgaac gtggcgagct gtgcgcgcac ggtgtcgacg acatcgtcct catagaccac
     5281 aggttctgcg tcgacaccga catcggcgag cgccgcgaag acggccttga accggctggt
     5341 ctctggcgag gcactgcggc gcgctgcttc atcgcctcgc cagaggatag cgatgcggcc
     5401 gagtcgagct gtgggatgaa acatggaaaa cggctccggg tttcattttt ctatgtgata
     5461 gcgtgtccgt atacgttcag caccaggttg ggcggctgcg aagccggcca ggccggatgc
     5521 gaaggcaacc aagacgccgc gaagagaaaa cactccaagg acaaagtctt ccatgcctcg
     5581 cttccatcat tgaggcgagc agcgcttggc agcgatccat caagagaggg ggacgcaatg
     5641 tcccctgatc tgcaatgtgt ctagtgattg aaaaaatcag ggccctgcac gcatgtgtcc
     5701 cctccttacg aaggagacgg tgcttgggcc cgcaagcctg gaagaacggg gcgaccgtcg
     5761 aaggccccgc gtaacgactt cactagctta ccatgttctg gggcgcaatc gatatgttgt
     5821 tcgctttgat cgagaacggc agatcgtagc tttgcgccgg atgtccgctc ggggtattta
     5881 ggaagttaat tgcaatcttc actcggcggc atcgccagcg atccgatcca ggcgcaatcg
     5941 atgccgatga tccgtgagca gatgtccgcc tcctatgacg gcggccagcg ttcccaggcc
     6001 cgttccgccg gcaatcaaga acatgatgag gagctgatac ttcacggcat cggtcggctc
     6061 gacgccggca agaatctggc ccgtcatcat gcccggcagg gaaacaagtc caattgccgc
     6121 catgccgtta atggtcggca tgaaaccgct tcgcaaagca tcccggatta tcggtagcag
     6181 cgcctgatac ctcgtgccgc ccagcgcaag acaagcttct accgcggctc gttcgcgcac
     6241 caaaccgttg gtgagcacgt caaggcccag gctcacaccg gtcatggtgt tacccaggat
     6301 catgcccaac aggggcagcg cgtagcgcgg atggtaccag ggttcgggac gcagctgcgt
     6361 gaggagagaa aacgtcgtca ccgtacccgc tgccagcaaa gtgcatcccg caccgagacc
     6421 gtacgtccaa aagcctcgca gtcgtcgctt ctgccgcgcc acagtttccc gcgacgcgaa
     6481 gaggaccatg atgatggccg cgaccgcagt ccagagtgga gagaccgagg caaacaggaa
     6541 tgtgagcaca tat
//
LOCUS       DATLJT010001260         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3545641,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001260 DATLJT010000000
VERSION     DATLJT010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545641"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VM910_28920"
     CDS             complement(<1..330)
                     /locus_tag="VM910_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66568.1"
                     /translation="MKIRTIIAATCAALAFSMAGPSYAHDSEAETVTKNFEAAIPNIR
                     GKSLIALDVDYAPGAASVPHTHAKSAFIYAYVISGAIESKVNDGETRIYRAGESWSET
                     PGAIHSIS"
     gene            complement(403..1857)
                     /locus_tag="VM910_28925"
     CDS             complement(403..1857)
                     /locus_tag="VM910_28925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66569.1"
                     /translation="MSSAPLILTDGGLALSEPKTVGAQGSTKVLAASSLGSCLVFVSS
                     AVVTVALAAIGRDMRLSPLDLQWVMNAELLPLAALTLVAGALGDRFGQKRIFLAGIAL
                     YGLGAAAIGFAPSFAPLIVGRFLLGLGGALILPNGLSVLGQAFPADKKARAVGIWSAA
                     AAVASGVAPAIAGAILDRGSWRTTFLMLLPAVAGALAVGAVWIPKDSLTSHTRVDVGG
                     AVFSTVGLGGLGAGLTSLTNGSGLNRWVLVALIVGLGGLACLIVTEWRLGDNAMLPPS
                     LFASRSVVGANLFTALLYGPFTIMLTLIPFVMIRGAQLPTLVAGLAFIPLQVLITIVS
                     PLAGMLCRRFGRRLPLFAGGTVVALGCAMALRVGSNATYWADIFPPIVLLSLGMSLVI
                     APLTTLVLTSVASDRAGTASGVNSAVARAGSLFAIALLGGVLQHGGPQLFSGFHMAIA
                     VATIACVLAAFAVFIIEPGPHVDFIPRDYPVRRQ"
     gene            complement(1986..2116)
                     /locus_tag="VM910_28930"
                     /pseudo
     CDS             complement(1986..2116)
                     /locus_tag="VM910_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528391.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            complement(2118..>2547)
                     /locus_tag="VM910_28935"
     CDS             complement(2118..>2547)
                     /locus_tag="VM910_28935"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HVI66570.1"
                     /translation="NQHEQETLMSESFPLPTEAEGVIPSLLKRFNSGKIEAMMALYAP
                     EAVFVANDGRTITDRAEFTAILEHDIKLGLPLKANVRHVFVGGDTAQIVLDWSIDGTG
                     PDGKIVHMHGTASDIMRRSADGFWRYLIDNNQGTAVRQPA"
BASE COUNT          496 a          799 c          845 g          407 t
ORIGIN      
        1 gctgatcgaa tggatcgcac ccggcgtttc ggaccagctc tcacccgccc gatagatgcg
       61 cgtctcgccg tcattcactt tcgactcgat cgcgcccgag atcacatagg catagatgaa
      121 ggccgacttc gcgtgggtgt gaggcacgga ggccgcgccg ggggcgtaat cgacatccaa
      181 ggcgatcagc gacttgccgc ggatattggg gatggccgcc tcgaagttct tcgtgacggt
      241 ttccgcttcg ctatcatggg cgtagctggg gccggccatc gagaaggcga gggctgcgca
      301 ggtggcggcg atgatggttc ggatcttcat gggctttctc cttctgccta taggttggtc
      361 ttcgttcttc attgtttcct cccggcctcg cgggcctgct gattactgac gccgaacggg
      421 atagtctcgc gggatgaagt cgacgtgggg ccccggctca atgatgaaca ccgcgaacgc
      481 ggcgagaacg cacgcgatcg tggcgacagc aatcgccatg tggaatcctg agaacagctg
      541 aggaccgcca tgctgcagga cgccgccgag cagggcgatg gcgaagagcg acccggcgcg
      601 cgcgaccgcg ctgttgacac cagacgcagt tccggcgcga tcggacgcga cggaggtgag
      661 gacgagggtc gtcaatggcg cgatcaccag gctcatgcct agcgacagca agacgatcgg
      721 gggaaagatg tccgcccaat aggtcgcgtt cgaaccgaca cggagcgcca tggcgcatcc
      781 caaggccacg acggtgccgc cggcgaacaa tggcaagcgc cggccgaacc ggcggcagag
      841 catgccggcg agcggcgaga cgatcgtaat cagcacttgc aggggaatga aagccaggcc
      901 cgccaccagc gtcggcagtt gcgcgccccg gatcattacg aacgggatca aggttagcat
      961 gatagtgaac ggcccgtaga ggagcgcggt gaacaggttc gcgccgacga ccgaccgcga
     1021 ggcaaagagg gacgggggca gcatggcgtt gtcgcctaat cgccattcgg tcacgatgag
     1081 gcaggccaag ccgcccagac cgacgatcag ggcgacgagc acccaacggt tcagaccgga
     1141 accgttggtc aggctggtca gccccgcgcc cagcccgccg agcccgactg tcgaaaagac
     1201 cgcgccaccg acgtcgaccc gggtgtggct ggtcagggag tccttgggta tccagacggc
     1261 gccgacggct agcgcgccgg cgactgcggg caggagcatc agaaaagtcg tccgccaaga
     1321 cccgcgatcg aggattgcgc cggcgatcgc gggagcgacg ccgctggcga cagccgcggc
     1381 ggcagaccag atgccgaccg cacgggcttt cttgtcggcg ggaaaggcct gcccgagcac
     1441 ggaaaggccg ttgggcagga tcaaggctcc gcccaagcct agcaggaacc ggccaacgat
     1501 gagcggcgcg aaactcggcg cgaaaccaat cgcggccgcg cccaggccat agagagcgat
     1561 ccccgcgagg aagatccgct tctgtccgaa ccggtcaccc agagcgccgg cgaccaaggt
     1621 cagggccgcg agcggtagga gctcggcgtt catcacccat tgcaggtcga gcggggacag
     1681 gcgcatgtct cggccaatcg ccgcgagcgc gactgtgacg acggcgctgc tgacgaatac
     1741 gaggcagctg cccagactcg acgcagcgag cacctttgtg ctgccctgcg cgccgacggt
     1801 cttaggctcg gagagcgcga ggccgccgtc ggtcaggatc aggggcgcgc tgctcacgac
     1861 ccaattcctc aggataggtc gcgcgattcg ccgcgcgaac tcattggcca gcagcctgaa
     1921 gccgcgccgc cgtgtccgtt cgcggatcac tcctcgcaac atcaattcaa cctcaagcac
     1981 accttctaaa ccgccgtcac ttgcagcccg cgctgcaccg ccggccgcgc gcgaggccac
     2041 ggttgagcca cgcctgcata cgaagaaggc ggtcgaagcc gaggagctcg tgcgcttcgt
     2101 aaaagtcgat aagattgtca ggcgggctgt cgcactgccg tcccttgatt gttgtcgatc
     2161 aggtaccgcc agaacccatc cgcgctgcgc cgcatgatgt cgcttgctgt gccgtgcatg
     2221 tgcacgatct tgccgtcagg acctgttccg tcaattgacc agtccagcac aatctgggcg
     2281 gtgtcgccgc cgacgaacac atggcgcaca ttagccttca gcggcaggcc gagcttgata
     2341 tcgtgctcga gtatggccgt gaactcggcc cgatcagtga tggttcgccc gtcgttcgcg
     2401 acgaataccg cctccggggc gtacaaggcc atcattgcct cgatctttcc ggagttaaat
     2461 cgcttgagca gagacgggat aacgccttcg gcttcagttg ggaggggaaa gctctcggac
     2521 atcagggtct cctgttcgtg ttggttg
//
LOCUS       DATLJT010001261         6606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2396031,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001261 DATLJT010000000
VERSION     DATLJT010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396031"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VM910_28940"
     CDS             <1..1989
                     /locus_tag="VM910_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI66571.1"
                     /translation="VADELDVPLNRIKLVQGDTALTPDQGTTWGSLSIQIGGMQLRNA
                     AATAKATLLDEAAKRLGAKQDELKVVDGVVSAGNKRVSYGELVGGKSFALKLDHTKPA
                     AGKDPKDYKLVGKPVPRVDIPDKVTGRFTYMQDFRVPGMLHGRVVRPPAIGATLESVD
                     EASIKDVVGVVKVVREGNFLGVIAESEWGAIKAAEKLKASWSKSQTLPDAAKLWDEVR
                     ASKVVKDEVTSNVGNTAEAMGKAGVKTLKATYDFAIHTHGSIGPSCAVAEFKDGKLTS
                     WSASQATHNLRKQLATMFGMPADSVRCIYIEGSGCYGRNGHEDAAADAALLAKTVERP
                     VRVQWSRADEHGWDPKGPPTLVDIRAAVDGDVVTAWESEFFIPQQTAGSFNVPLVAAT
                     LAGMPAGDDIAPGNIFQNSAIPYKFANVKTVCRRLATTPFRPSWIRTPGRMQNTYANE
                     CFIDELAAAANADPVEFRLKYLDPNDKRGIEVLNRAAALAKWDKRPSPRRDQRGDLAT
                     GRGVAYCKYELARTYIAAVAEVEVKRSTGDIRVTKFYLAHDCGQIVNPDGLRNQLDGN
                     VIQTISRTLMEELKYDRSAVTSLDWESYPILRFPQLPELVYDLIDRPNERPWGAGEPA
                     AAVVPSAISNAVFDAIGVRLRSVPFTPDKVKAAMKGAA"
     gene            2185..3450
                     /gene="phnA"
                     /locus_tag="VM910_28945"
     CDS             2185..3450
                     /gene="phnA"
                     /locus_tag="VM910_28945"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HVI66572.1"
                     /translation="MTSARTSEIRLNGVRYRWPDRPVVVICNDGGDPAYIDRALKDGI
                     VPNIARFMQTGFNAVAECVIPSFTCPNNVSIITGSPPSIHGVSGNFYLDPETRKAVVM
                     TGPELMRSTTILAEFSRNGAKVASVTAKDKLRYQLGKDMNIADGSINFSSEKADKCSK
                     CENGIENVLEFVGMPLPDMYSPELSLFVLETGIKLLQIEKPLVMYLSLTDYIQHKYAP
                     GEPEANRYYRDMDAMLGRLHDLGPVIGLIADHGMCDKSNPDGSRRIVWLQDVLDLEFG
                     KNSTTVICPITDYFVAHHGSLGSFVRVYCHDRTTPEAVMKLARKLPGIEAVYDKTTAA
                     RRFDLPIDREGDVVVISDADTCIGTTPSAHNLSALDGHALRTHGGIAEGRVPFILNYP
                     LNETYAARAARGGLKNFHIFDFAINGVVT"
     gene            3570..4634
                     /locus_tag="VM910_28950"
     CDS             3570..4634
                     /locus_tag="VM910_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI66573.1"
                     /translation="MHNQLQRRMFLTGSAALGIASFTPRVSFADTVPATATGPALIAA
                     AEKEGKVVWYTSVDLPVAEKVARGFETKHPAISVRVERNGSERLFQRIEQELASGIHA
                     GDLVNTSDGAHFVIWKRTGRLEQYLPIEAAQFLPKDQIDPDSTFMSWRASLSVIAYNS
                     NLVKSEEAPRSFADLLHPRWLGKIVKGHPGYSGTILTATFQITRELGWDYLERLAKQR
                     VLQVQSSTDPPKKLVLGERAVMADGNEYNVFQMKESGQPLDVVYATEGTPLITGPSAI
                     FKTAPNPNAARLFQHYLFAPETQQAICDLGGLRSFYPMVKEKSGRRSLREIRLMNDDP
                     VAIAEQSENIKARYIQIFRV"
     gene            5292..6293
                     /locus_tag="VM910_28955"
     CDS             5292..6293
                     /locus_tag="VM910_28955"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66574.1"
                     /translation="MTVTIGRRKLLAAFGGAAAAWPLAASAQQPGTLRRIGVLIQVAE
                     GDPQARIEVATFLRGLQELGWNEGRNLRIDTRWGGGDADRIRKYAAELVALGPEVVLA
                     PGGTVVGALQQASRTVPIVFVNVTDPVGRGYVASLAQPVGNATGFTSFEFGMGGKWLE
                     VLKEMAPRVTRAAVLRDPVITAGMGYLAAIHALAPSVGVQVTPVDVRHTSDIERAVAA
                     FARTPNSGLIVTADPAAIVHREGIIALAARHRLPAVYPYRFFVISGGLISYGLDNIEQ
                     YRLAAGYVDRILKGDKPGDLPVQAPTKYELVINLKTAKTLGLEVPPTLLARADEVIE"
     gene            6290..>6606
                     /locus_tag="VM910_28960"
     CDS             6290..>6606
                     /locus_tag="VM910_28960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66575.1"
                     /translation="MTRREFITVVGGTAAAWPLAARAQQRERMRRIGVLTNLSSDDPA
                     QQSRVLAFAQTLAQWGWTEGRNVRIDIRWGATDPERVRRYVAETVALAPDVILAVGSA
                     ATGG"
BASE COUNT         1353 a         1988 c         2077 g         1188 t
ORIGIN      
        1 gtcgccgacg agctcgacgt gccgctcaac cggatcaagc tcgtgcaggg cgacaccgcg
       61 ctcacgcccg accagggcac cacctggggc agcctctcga tccagatcgg aggcatgcag
      121 ctgcgcaacg cggcggcgac agccaaggcc acgctcctcg atgaggcggc caaacgcctc
      181 ggcgccaagc aggacgaact caaggttgtg gacggcgtgg tcagtgccgg caacaaacgg
      241 gtgagctacg gcgagctggt cgggggaaag tcattcgcgc tcaagctcga tcacaccaag
      301 cccgcggccg gcaaggaccc gaaggactac aagctcgttg gcaaaccggt gccgcgcgtc
      361 gacatcccgg acaaagtcac gggtcgtttc acctacatgc aggacttccg cgtgcccggc
      421 atgctgcatg ggcgcgtcgt gcgcccgccg gcgatcggcg ccacgctcga aagcgtcgat
      481 gaggcttcga tcaaggacgt tgtcggggtg gtcaaggtcg tgcgcgaggg caatttcctc
      541 ggcgtcattg cggagagcga gtggggcgcg atcaaggcgg cggagaagct caaggcctca
      601 tggtcgaaat cgcagacgct gccggacgcc gccaagctgt gggatgaggt gcgcgcgagc
      661 aaggtcgtca aggacgaggt gacgagcaac gtcggcaata ccgccgaggc catgggcaag
      721 gcgggcgtca agacgctgaa ggccacctac gactttgcca tccacaccca cggttcgatc
      781 gggccgtcct gcgcggtggc cgagttcaag gacggcaagc tgacgtcctg gtcggcctcg
      841 caagcaaccc acaatctgcg caagcagctc gccaccatgt tcggcatgcc cgccgacagc
      901 gtgcgctgca tatatatcga aggctccggc tgctatggcc gcaacgggca cgaggatgcg
      961 gcggcggacg cggcgctgct ggcgaaaacg gtcgagcggc cggtgcgggt gcaatggtcg
     1021 cgcgcggacg agcacggctg ggaccccaag ggtccgccga cgctcgtcga catccgcgcc
     1081 gccgtggatg gcgacgtcgt caccgcctgg gaatcggagt tcttcattcc gcagcagacc
     1141 gccggcagct ttaacgtgcc actggtcgcc gcgacgcttg cgggcatgcc ggctggcgat
     1201 gacatcgcgc ccggcaacat tttccagaac tcggcgatcc cgtacaaatt cgccaacgtc
     1261 aagacggtgt gccgccggct cgcaaccacg ccgttccgcc cctcctggat ccgcacgccg
     1321 gggcggatgc agaacaccta tgccaacgag tgctttatcg acgagctcgc cgcggcggcg
     1381 aacgccgacc ccgtcgaatt ccggctgaag tatcttgatc ctaacgacaa gcgcgggatc
     1441 gaagtgctca accgcgccgc cgccctggca aaatgggaca agcggccttc gccccggcgc
     1501 gatcagcgcg gcgacctggc cacgggccgc ggcgtcgcct attgcaaata cgagctggcg
     1561 cgcacctaca tcgcggcggt ggccgaggtc gaggtgaagc ggtcgaccgg cgacatccgg
     1621 gtgacgaaat tctacctcgc gcatgactgc ggtcagatcg tcaatccgga cgggctcaga
     1681 aaccagctcg acggcaacgt gatccagacc atcagccgca ccttgatgga ggagctcaaa
     1741 tacgaccggt ccgcggtgac gagcctcgac tgggagagct atcccatcct gcggttcccg
     1801 cagctccccg agctggtcta cgacctgatc gatcggccga acgagcgacc gtggggcgcc
     1861 ggcgagcccg ccgctgcggt cgtgccctcg gcgatctcga atgcggtttt tgacgcgatt
     1921 ggcgtacgcc tgcgttccgt gcccttcacg ccagacaagg tcaaggcagc gatgaaggga
     1981 gcggcgtgat cgagaaggcg gaggctcgct ccgcctcatg ctgaagcgtc tctaagcgct
     2041 gcaagtcggc gagcgcgcca gttcgcgcac tcaatgctgt gcagatgtcg ggtacctcga
     2101 ggtcgcgggc cgtccccatt gtccctggcg cccctctcgg acatattggt agcgtaggcc
     2161 gcccgagatc ggcatgggca gcaaatgaca tccgctcgca cctcggagat tcgtctcaac
     2221 ggcgtacgat accgctggcc cgaccgaccc gttgtggtca tttgcaatga cggcggcgat
     2281 ccagcctata tcgatcgggc gcttaaagat ggaatcgtgc cgaacatcgc tcgctttatg
     2341 cagacggggt tcaacgcggt cgccgaatgc gttattccga gcttcacctg ccccaacaac
     2401 gtgtcgatta tcaccggcag cccgccatcg atccacggtg tatccgggaa tttctatctc
     2461 gatccggaga cgagaaaggc ggtggtcatg actggtcccg agctgatgcg ctcgacgacg
     2521 attcttgcgg agttctcgcg caacggcgct aaggttgcat cggtcacggc aaaggacaag
     2581 ctgcgctatc agctcgggaa ggacatgaac atagcggatg gaagcatcaa tttctcctcg
     2641 gaaaaggcgg ataaatgcag caagtgcgag aatggtatcg agaacgtcct tgagttcgtt
     2701 ggcatgccgc ttccggatat gtattcgcca gaactgtccc tgttcgtgct tgagacgggt
     2761 atcaagctcc tgcaaataga aaagccgctt gtaatgtact tgtcgctgac agactacatc
     2821 caacacaaat acgcgcccgg tgagccggaa gcgaaccgtt attatcgcga tatggacgcg
     2881 atgttgggac gtcttcacga tctcggcccg gtcatcggtc tcatcgcgga ccacggcatg
     2941 tgcgacaagt caaatcctga cggttcgcgc agaatcgtgt ggcttcagga cgtgcttgat
     3001 cttgagttcg gcaagaactc aaccacggtg atctgcccga tcaccgatta tttcgtagct
     3061 catcatggct cgctcggcag cttcgtgcgc gtctattgtc acgatcgtac aacacccgaa
     3121 gccgtgatga agctcgcccg aaagcttcct ggtatcgaag ccgtttacga caagaccacc
     3181 gccgctcgta gattcgatct gccgatcgat cgtgaaggcg acgttgtggt cattagtgat
     3241 gccgacacct gcatcggcac gaccccttcc gcacacaacc tttccgctct cgacggccat
     3301 gcgttgcgaa cacatggtgg catcgccgag ggtcgcgtcc cattcatact gaactatccg
     3361 ctgaacgaaa cttatgcggc gcgggctgca cgaggcggct tgaagaactt ccatatcttc
     3421 gacttcgcca taaacggcgt cgtgacctga gaagatcgct gctcgaagca acccgacggc
     3481 acgggaagaa agcggagtaa tgtgcgctaa gaacgcgtca aaagcagacc gccctgaggt
     3541 caaattatct gaacgcatgg acgctacaaa tgcacaacca acttcaacgc agaatgttct
     3601 tgacgggatc ggcggcgctc ggcattgcct cgtttacgcc ccgagtctcg tttgcggata
     3661 ccgttcctgc cactgcgaca gggcctgccc tgattgcagc agcagagaag gaaggaaagg
     3721 tcgtctggta tacgtcagtc gacttgccag tggccgagaa agtagcaaga ggcttcgaga
     3781 ccaaacatcc agctatttct gttcgagtcg agcgcaacgg ctcagagcgc ctcttccagc
     3841 gcattgagca ggaactggct agcggcattc atgccggcga tttggtgaac acgtcggatg
     3901 gcgctcactt tgtaatatgg aaacgaacgg gacgtctgga acaataccta ccgatcgagg
     3961 cggcacagtt tctgcccaag gatcaaatcg atccggacag cacattcatg agttggcgtg
     4021 cgtccctaag cgtgattgcc tataactcga acttggtaaa atcggaggaa gcaccgagga
     4081 gtttcgccga tcttctccat cccagatggc taggtaagat cgtgaaaggc catcctggct
     4141 acagcggcac gatcctgaca gcgacgtttc agataacccg cgaacttggt tgggactatc
     4201 ttgagagact cgccaagcag cgcgtcctac aagtgcaaag ttccactgat ccaccaaaga
     4261 aacttgtgtt gggggagcgt gcggtcatgg cagacggcaa cgaatacaac gtcttccaaa
     4321 tgaaggaatc gggtcaacca ctggacgtcg tctacgcgac cgaaggcacg ccattgatta
     4381 cagggccgag cgccatattc aaaaccgcac ccaatccgaa tgccgctcgg ctattccagc
     4441 actatctttt cgcgccggag actcaacaag caatttgtga tctgggtggg ctacgctcct
     4501 tctatccgat ggtaaaggaa aaatccggac gcaggtcctt acgtgaaatt agattgatga
     4561 atgatgatcc ggtcgctatc gcagagcaga gcgaaaacat taaagcgcgt tatatccaga
     4621 tattccgggt ctagcggctg gtgacgggaa aacccggagc gcctgatcga gacagtgttc
     4681 tcgcccgtcc ggcgagtgga acgacgacga tttcgacgtg ctggcggacg gcggagtggt
     4741 cgggcgcatc atgaaagctg cgtccgcgcc ggtaggctag ccatggctgt gggacgctgg
     4801 cttatggcca ccacgaagac cgcacgccga tttacggcta cgagccaacg cgcgaggctg
     4861 cgatggcggc gctcgccaaa gagctggcgg cgggagtagg ccgtggcaaa ggagactacg
     4921 aagctcatcg cacgccgcga tcggcatcag ccacgtcgca ctcggcatca ccgcgcatgc
     4981 gtccggcacc gctgacggac ctgccgggcc gttatcgtct cggcattgcc gattgccgtt
     5041 attgggcggg ccgtgtcgcc ctcagagaaa acccgcgcgc gcacacacgt cgtctcgcag
     5101 gtccgagagg aattgaagcg acgcggctga aaataaatct cttgaaatca agccgggggt
     5161 cgtggccggg cggcgatccg ttgaggccgg tgcccggcgt gccgccccgc ccacgccggg
     5221 ggccacgcag ttaggacagt gggctcgtgg cgtggtatcg ttgcatttac gtaccctcgc
     5281 gggaggggcg tatgacagtc accatcggac ggcggaaatt gctggccgca tttggcggcg
     5341 cagcagcggc gtggccgctc gcggcaagcg cgcagcagcc gggcacgctt cggcgcatcg
     5401 gcgtgctcat tcaagttgcc gagggcgatc cgcaggctcg catcgaggtc gcgacgtttc
     5461 tgcgaggcct gcaggaattg ggctggaacg aaggtcgcaa ccttcggatc gatacacgct
     5521 ggggcggggg cgatgctgat cgcattcgta aatacgcggc ggaactagtc gcacttggcc
     5581 cggaagtcgt cctggcaccg gggggcacag tagtgggggc gctgcaacag gcgagccgca
     5641 ccgtgccaat cgtgttcgtg aatgtcaccg atccggtcgg ccgcggctat gtcgcaagtc
     5701 tggcacaacc agtgggcaac gcgactggat ttacctcttt cgaattcggg atgggcggaa
     5761 aatggctgga ggtgctcaag gaaatggcgc cgcgagtgac ccgcgcggcg gtacttcgcg
     5821 atcccgtcat caccgccgga atgggttact tggctgcgat ccacgccctg gcgccgtccg
     5881 ttggggtgca agtgaccccg gtcgatgtgc gccacactag cgacatcgaa cgtgctgtcg
     5941 cggcgttcgc gcgcacgccg aatagtggcc tcatcgtgac ggcagacccg gcggcaatcg
     6001 ttcatcgcga aggaatcatc gcgcttgctg ctcgacatcg gttgcccgca gtctatccct
     6061 accgcttctt cgtcatcagc ggcggattga tctcttatgg gcttgataac atcgagcagt
     6121 accggctcgc agcgggctac gtcgaccgca tcctcaaggg cgacaaaccc ggagacctgc
     6181 cggtgcaagc gccgaccaag tacgagctgg tgatcaacct caagaccgca aagacacttg
     6241 gcctggaggt gccgccgacg ctgctcgcgc gcgctgacga ggtgatcgaa tgacgcggcg
     6301 tgagttcatc acggtggttg gcggcacggc ggctgcgtgg ccgctcgcgg cgcgcgcgca
     6361 gcagcgcgag cgcatgcggc gcatcggcgt gctgaccaac ctctcgtcgg acgatccggc
     6421 acagcagagc cgcgttctgg catttgcaca gacgctggca caatggggct ggaccgaggg
     6481 tcgcaatgtt cgcattgata ttcgctgggg tgcgactgac cccgagcgcg ttcgcagata
     6541 cgtggcggaa acggttgccc tcgcccctga cgttatcttg gctgttggca gtgcagccac
     6601 gggggg
//
LOCUS       DATLJT010001262         1606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_97866,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001262 DATLJT010000000
VERSION     DATLJT010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97866"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VM910_28965"
     CDS             <1..312
                     /locus_tag="VM910_28965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561870.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HVI66576.1"
                     /translation="ATYRRKQQEAEHEAQAIVAGARTEAERLAAEAEAKIEEFVVRRT
                     KMAENKIAQAEAQALADVRSAAAEVAVAAAKTILTRTVKDKVADNLLAKGIEDFKGKL
                     N"
     gene            506..1159
                     /locus_tag="VM910_28970"
     CDS             506..1159
                     /locus_tag="VM910_28970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVI66577.1"
                     /translation="MVIFDCNGVLVDSEQIAAAAAADEFARAGIPITPELVTRYFSGR
                     RPADMLAGIEAATGRKLPVNFSATLAAATLARLRAEVRAMPHAVHALTWLRGPKCVAS
                     SSPPDRVRASLETTGLAKFFNFLFSASDVRNGKPAPDLFLHAASRMGVQAADCIVVED
                     SPAGVAAATAAGMAALGFVGGSHAGAQLGEQLILAGARMIVADLRHLKSAVVALRGW"
     gene            complement(1176..>1606)
                     /locus_tag="VM910_28975"
     CDS             complement(1176..>1606)
                     /locus_tag="VM910_28975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66578.1"
                     /translation="NIAAKAADATAGDLRYQLSFGQTARECVVQGATLIIKVGVQGRV
                     ILGPMGSPGQVDVPLRYAVVREGPQPKTVATKFKRIAVTIPLGETHVPFVDIEEGLAF
                     PMPSRSELDAYVVYVGFDEIGDKNEKKPPKTARKTTTRQQ"
BASE COUNT          300 a          517 c          494 g          295 t
ORIGIN      
        1 gccacatatc gacgcaagca gcaggaagcc gagcacgagg cgcaggcgat cgtcgccggg
       61 gccagaacag aggccgagcg gcttgccgcc gaggccgagg ccaagatcga agaattcgtc
      121 gtgcggcgta ccaagatggc ggaaaacaaa atcgctcagg ccgaagctca ggcgctggcc
      181 gacgtgcgct ctgcggccgc agaggtcgcg gtcgccgcag ccaaaacgat cctcacccgc
      241 accgtcaagg acaaggttgc cgacaatctc cttgccaaag gcatcgaaga cttcaagggc
      301 aagctaaact agtctttttt ccggccactt tcggccacga cgcaagatta ttccggcttt
      361 cccggcaaaa cgcagaagct gtgtaaaaaa atctctcggg cgcgcggcgc ccgacttttg
      421 ccatgggctc acgattcctt cggttgcgcc gccgcgattc gttgaaacca ccgcggtgct
      481 gcaagcacgc cggcgtgagg ctcccgtggt tatttttgat tgcaacggtg tgctggtcga
      541 cagcgaacaa atcgccgcag cggctgccgc ggacgaattt gcgcgggccg gaattccgat
      601 tacgcccgaa ctcgtcactc gctatttctc gggccgccgt ccggccgaca tgctcgctgg
      661 catcgaagca gccaccggcc gcaagctgcc ggtcaatttc agcgccaccc tcgcggcggc
      721 aacgctggcg cgcctacgcg cagaggtacg cgccatgcca catgcagtcc acgcgctcac
      781 ctggctgcgc gggccgaaat gtgtggcctc atcctcgccc cctgaccgtg ttcgggcaag
      841 cctggaaacc accgggctcg cgaagttctt caactttcta ttttccgcaa gcgacgtccg
      901 caacggcaag cccgcccccg atctattcct gcacgcagcg tcgcggatgg gggttcaagc
      961 cgccgattgc atcgtagtcg aggattcccc ggctggcgtc gctgcggcca cggcggcggg
     1021 tatggccgca ctcggatttg ttgggggcag ccatgcgggg gcgcagcttg gcgagcagct
     1081 gatattggcg ggagcgcgga tgatcgttgc tgacctgcgg catctcaaga gcgccgtggt
     1141 cgcactccgc gggtggtaga gaagaccggg atcgtctatt gctgccgtgt cgtcgtcttc
     1201 ctcgccgttt tcggcggctt cttctcgttc ttgtccccga tctcgtcgaa accgacatag
     1261 acgacataag catcgagttc cgacctcgat ggcatcggga acgccaaacc ttcctcgata
     1321 tcgacgaatg ggacgtgggt ctcgcccagc ggaatggtca ccgcaatccg cttgaatttg
     1381 gtcgcgacgg tcttgggctg cggaccctcg cggactaccg catagcgcaa cggaacgtcg
     1441 acctgccccg gactgcccat aggtccgaga atgacgcggc cctgtacgcc gactttgatg
     1501 atcagagtag caccctgcac cacgcactcg cgggcggttt ggccgaacga aagctggtag
     1561 cgcaggtcgc ctgcggtcgc atcggcggcc ttggccgcga tattca
//
LOCUS       DATLJT010001263         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4233922,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001263 DATLJT010000000
VERSION     DATLJT010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4233922"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VM910_28980"
     CDS             <1..472
                     /locus_tag="VM910_28980"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HVI66579.1"
                     /translation="FIVFAASGGARMQEGILSLMQLPRTTVAAQMLREAKLPYIVVLT
                     NPTTGGVTASYAMLGDIQIAEPGALIGFAGARVIEQTIREKLPEGFQRAEYLRDHGMI
                     DMVVHRHDLRPTLARLCRLLTRSPAIETAPKAAPLVPAAAIVSAPETAPAAPYA"
     gene            469..1797
                     /locus_tag="VM910_28985"
     CDS             469..1797
                     /locus_tag="VM910_28985"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HVI66580.1"
                     /translation="MNAPAAKSPTPLDEVVARLSQLHPKRIDLSLERMHRLLDRLDHP
                     ERKLPPVIHVAGTNGKGSTVAYLRAILETAGLKVHAYTSPYLLRINECFRLGGTLVGD
                     DELCGALEHCERVNAGAPITFFEAKTAAAFCLFAQHAADMLILEVGLGGRLDSTNVID
                     NPLACAIAPIGMDHTEYLGDTLTAIAGEKAAIIKRGVPVITAEQAPEAMCVIEQQARR
                     QRAPLHAAGQEWHVGVERGRLVYQDDRGLMDLAAPKLFGRHQFDNAGLAIATLRAIDA
                     IRIPHAAFEGIVNAEWPARMQRLSSGRLAQFGARGCEIWLDGGHNADGGRVVAAALGD
                     LEERVSRPLVVIVGMMATKDAGAFLANFAGLTRHIIAVPIPDQDNAMQPDRLADAARS
                     LGMRVEIAGSTEAALRRLAGLAYEVPPRILITGSLYLAGPVLALNGTPPK"
     gene            complement(1874..2194)
                     /gene="trxA"
                     /locus_tag="VM910_28990"
     CDS             complement(1874..2194)
                     /gene="trxA"
                     /locus_tag="VM910_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVI66581.1"
                     /translation="MAVGKVSDANFEAEVLKATGPVVVDFWAEWCGPCRMIAPALDEI
                     AGAMGDKVKIVKLNVDESPKTASKYGVMSIPTLMIFKGGEMASRQVGAAPKQKLHQWI
                     TAAV"
BASE COUNT          407 a          760 c          751 g          409 t
ORIGIN      
        1 gttcatcgtg tttgccgctt ccggcggcgc gcgcatgcag gagggcatcc tgtcgctgat
       61 gcaactgccg cgcacgacgg ttgcagcgca gatgctgcgc gaggcgaaac tgccctacat
      121 cgttgtgctg accaatccga ctaccggcgg cgtcaccgcc tcctatgcca tgctcgggga
      181 catccagatc gccgagcccg gtgcgctgat cggctttgcc ggcgcgcggg tgatcgagca
      241 gaccattcgc gagaaattgc cggaaggttt ccagcgggca gagtatttgc gcgatcacgg
      301 catgatcgac atggtcgtgc accgacacga tctccgcccg acgctggcgc ggctgtgccg
      361 cctgttgacc aggtcgccgg cgatcgagac ggcgccgaag gcggcgccgc tggttccggc
      421 agccgcaatc gtatcggcgc ccgaaacggc gccggctgcg ccctacgcgt gaacgcgccc
      481 gccgccaaat cgccaacgcc gctcgacgaa gtggtcgcgc ggctgtcgca gttgcatccg
      541 aagcgcatcg acctgtccct cgagcgcatg catcgcttgc tggatcggct cgatcatccc
      601 gaacggaagc tgccgccggt catccacgtc gccggcacga acggcaaggg ctcgacggtc
      661 gcctacttgc gcgcgatcct ggaaaccgca ggcctcaagg tccacgccta cacctcgccc
      721 tatctcttgc gcatcaacga atgtttccgc ctcggcggga cgctcgtcgg cgacgacgaa
      781 ttatgtggcg cgctggagca ttgcgagcgc gtcaatgccg gcgcgccgat cacattcttc
      841 gaagcgaaga ccgctgcggc tttttgcctg ttcgcgcaac atgcggccga catgctgatc
      901 ctggaagtcg gtctcggcgg ccggctcgat tcgaccaatg tgatcgacaa tccgctcgcc
      961 tgtgcgatcg cgccgatcgg catggaccat accgagtatc tcggcgacac gctcaccgcg
     1021 attgccggcg agaaggccgc catcatcaag cgaggggtgc cggtgataac agccgagcaa
     1081 gcacccgagg cgatgtgcgt gatcgagcag caggcaagac ggcagcgcgc gccgctccac
     1141 gccgcaggcc aggagtggca tgtcggtgtc gaacgcgggc ggctggtcta tcaggacgat
     1201 cgtggcctga tggacctcgc cgcgccaaaa ttgttcggcc ggcaccagtt cgacaatgcg
     1261 gggcttgcca ttgccacgct gcgcgccatc gatgcgatca ggattccgca cgccgcgttc
     1321 gagggcatcg tcaatgccga gtggcccgcg cggatgcaga ggctttcgtc cggtcggctg
     1381 gcacaattcg gagcgcgggg ctgcgagatc tggctcgatg gcggtcacaa cgcagacggt
     1441 ggtcgggtgg tcgcagccgc gctcggcgac cttgaagagc gggtgtcgcg gccgctggtc
     1501 gtgatcgtcg gcatgatggc gaccaaggat gccggcgcgt tcctcgccaa ttttgccgga
     1561 ctgacgcgac acattatcgc ggtcccgatt ccggatcagg acaatgcgat gcaacccgac
     1621 cggctcgccg atgcggcgcg ctcgctcggc atgcgcgtgg agatcgccgg ctccaccgaa
     1681 gcggcgctgc ggcgcctcgc tggacttgcc tatgaagtgc cgccgcgcat cctgatcaca
     1741 ggctcgctct atctcgcggg tcccgtactg gcgctgaacg gcacgccgcc gaaatagagc
     1801 cggaccatgc gctttgatga aacaaaacgg ccggcgcgat ggccggccgt ggtgcaatcc
     1861 tggaagcgtc gactcagacc gcagcggtga tccactgatg cagcttctgc ttcggcgcag
     1921 cgccgacctg gcgagaagcc atctcgccgc ccttgaagat catcagggtc gggatcgaca
     1981 tgacgccgta tttcgaggcc gtcttcgggc tctcgtcgac gttcagcttc acgatcttga
     2041 ccttatcgcc catcgcgccg gcgatctcgt cgagtgcggg ggcgatcatg cggcacggac
     2101 cgcaccattc ggcccagaaa tccaccacca cagggccggt cgccttgagc acttcggcct
     2161 cgaaattggc gtcagaaacc ttgccaacgg ccatggaata cctcgtttgg ttggagagag
     2221 cggcgcgcta acggcctaag agattcgcgc ctgcaatgat gagtgtaaac ctatgaacga
     2281 ggcgttgccg ggtcaagttc gcatcctgcg aatgccagtt ccgcgtc
//
LOCUS       DATLJT010001264         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_560189,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001264 DATLJT010000000
VERSION     DATLJT010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560189"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1295
                     /locus_tag="VM910_28995"
     CDS             63..1295
                     /locus_tag="VM910_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HVI66582.1"
                     /translation="MVGPFNHRSGAAGRDVPGPRTLGATRRTAIGLMLGAPLLSACSG
                     IQQTFSQFTSSETPGPAGPAQQPSVAGTGQVKVGLILPMSASGNAGVAAQSMKNAAEM
                     ALAEFQNPNIQLLIKDDGGSPQGAAQGAQQALGEGAEIILGPLFALAVPSTAQAARSR
                     SVSVIAFSTDSSVAGRGVYLLSFLPESDVNRIVEYSASIGKKSFAALLPDNAYGNVVE
                     VAFKQAVGRRGGRIVAFEKYSADRATPARTVAQALGQADALLIADDGDSVVATADALT
                     AAGANLRNIQLLGTGLWDNPRVFASPSLQGGLYAAPDPSGFRAFAGRYRTRFGGEPVR
                     TATLAYDAVALVAALARAQGTQRYAPDVLTNPSGFAGIDGLFRFRPDGTNERGLAVMR
                     VGTGGGVAVAGSPKSFGA"
     gene            complement(1388..>2513)
                     /gene="hemW"
                     /locus_tag="VM910_29000"
     CDS             complement(1388..>2513)
                     /gene="hemW"
                     /locus_tag="VM910_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HVI66583.1"
                     /translation="YVHWPFCLSKCPYCDFNSHVRHAAIDEDRFARAFAREIETTAAR
                     VPGRQVSSIFLGGGTPSLMRPETVGAVLDAIAKHWRLDDGVEVTLEANPTSVEAARFA
                     GYRAAGVNRVSLGVQALDDASLKQLGRLHSVREALDAVAIARKAFERYSFDLIYARPD
                     QTADVWAAELQLAIKEAAEHLSLYQLTIEEGTPFFGLYAAGKLKTPNEATARALYDVT
                     QEVCAHHGLPAYEISNHARKGAECRHNLVYWRGEEYAGIGPGAHGRLDINGSRHATMA
                     EKRPEAWLMRVEANGHGVITNDVLNSAERADEFLLMGLRLVEGIDPRRYAALAGRPLN
                     PRRIALLREEGAIVVDGDGRLRVTQAGFPVLDAVVADLAA"
BASE COUNT          432 a          807 c          826 g          448 t
ORIGIN      
        1 ctcccgaccg tatttaaggg ggcagtcctg accggtcgag gtcgatcaag cgaagagatg
       61 tgatggtggg cccgttcaat cacaggtccg gcgctgcggg tcgtgacgtc ccgggtccgc
      121 gcacgcttgg tgcgacccgg cggacggcga tcgggctgat gctcggtgcg ccgctgcttt
      181 cggcttgttc cggcatccag cagaccttta gccagttcac gagctcggag acgccggggc
      241 cggctggacc ggcccagcag ccgagcgtcg ccggcacggg ccaggtcaag gtcggattga
      301 tcctgccgat gtctgcctcg ggcaatgccg gcgtcgccgc acaatccatg aagaacgcgg
      361 ccgaaatggc gcttgcggaa ttccagaatc ccaatatcca gcttctcatc aaggatgacg
      421 gcggcagtcc gcaaggcgcc gcccaaggcg cacagcaggc gctcggggaa ggtgcggaga
      481 tcatcctggg tcccctgttt gcgctcgcgg tcccatcgac cgcgcaggcg gcgcgctcgc
      541 gcagcgtctc ggtgattgcc ttttccaccg attcgagcgt tgccggccgc ggcgtctatc
      601 tcttgagctt cctcccggaa tccgacgtca atcgcatcgt cgaatactcc gcaagcatcg
      661 gcaaaaaatc cttcgcggcg ctgttacccg ataacgccta tggcaatgtg gtggaggtgg
      721 ccttcaagca ggcggtcggg cgcaggggcg ggcgtatcgt cgcgttcgaa aaatatagcg
      781 ccgaccgcgc gacgccggca cggacagtgg cgcaggcgct cgggcaagcc gacgcgctcc
      841 tgattgccga tgacggcgac tcggttgtcg cgacggccga tgccctcact gcggcaggcg
      901 ccaatctgcg gaacatccag ttgctcggca cggggctttg ggacaatcct cgcgtgtttg
      961 ccagtccttc cttgcagggc ggtctttatg cggcgccgga tccttccggc ttccgcgcct
     1021 ttgccgggcg ctaccgcacg cggttcggcg gcgaaccggt caggaccgct acactcgcct
     1081 acgatgccgt ggctcttgtc gcggcgctgg ctcgggcgca gggcacgcaa cgctacgcgc
     1141 cggatgtgct gaccaatcca tcaggctttg ccgggatcga tgggctgttc cgcttccggc
     1201 ccgacgggac caatgagcgg ggacttgccg tcatgcgggt cggcaccggc ggcggggtag
     1261 cggttgcggg ctcgccaaag agcttcggcg cttagcgcat gatccggaaa agtgcgcacc
     1321 ggtttttctc gcgacaaacg cggaacgcgt ttgcgcggag gtcatgggca aataatcagg
     1381 tcacccatca cgccgcgaga tcggcaacga ctgcatcaag caccggaaat ccggcctggg
     1441 tgacgcgcag ccggccgtcg ccgtcgacaa cgatggcgcc ctcttcgcgc aacagcgcga
     1501 tgcggcgtgg gttgagcggg cgcccggcga gtgccgcata gcgcctgggg tcaatgccct
     1561 cgacaagccg caagcccatc aacaaaaatt cgtcggcgcg ttcggcgctg ttgagaacat
     1621 cgttggtgat gacgccgtga ccgttcgcct cgacccgcat cagccaggct tcggggcgct
     1681 tttcggccat ggtggcgtgt cttgagccgt tgatgtcgag gcggccatgc gcgccggggc
     1741 cgatgcccgc atattcctcg ccgcgccaat acaccagatt gtgccggcac tccgcgcctt
     1801 tgcgcgcatg gttggaaatt tcgtaggccg gcagaccatg atgcgcgcag acctcctgcg
     1861 tgacgtcgta gagcgcgcgt gcggtcgctt cgttcggcgt cttcagcttg cccgcggcat
     1921 acaaaccgaa aaagggcgta ccttcctcga tcgtcaattg ataaagcgac agatgttcgg
     1981 ccgcctcctt gattgcgagc tgaagctcgg cagcccacac gtccgccgtc tggtccggac
     2041 gcgcatagat caggtcgaac gaatagcgct cgaaggcctt gcgcgcgatc gcaaccgcgt
     2101 cgagcgcttc gcgtacagag tgcagccgcc ccaattgctt cagtgatgca tcatcgagcg
     2161 cctgcacgcc aagcgaaacg cggttgacgc cggctgcgcg atagccggca aagcgcgcag
     2221 cttcgacgct ggtcgggttg gcctccaggg tgacttcgac gccatcatcc aggcgccagt
     2281 gttttgcgat cgcatcgagg accgcgccga cggtctcggg ccgcatcagc gatggcgtgc
     2341 cgccgccgag aaaaatcgaa gagacctgcc gccccggcac gcgcgcggcc gtggtttcga
     2401 tttcgcgcgc gaacgcgcgg gcaaagcgat cctcgtcgat cgcagcatgc cggacatggc
     2461 tgttgaaatc gcaataggga catttcgaca ggcagaacgg ccagtgcaca tag
//
LOCUS       DATLJT010001265         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2167080,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001265 DATLJT010000000
VERSION     DATLJT010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167080"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(643..1065)
                     /locus_tag="VM910_29005"
     CDS             complement(643..1065)
                     /locus_tag="VM910_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66584.1"
                     /translation="MCDYSLHAVATRPAQVGETLVTTTFRGTSTRGFASENDLSIAVC
                     LMPGTELAFADDVKYDNRWIWTRTINSKVGKFGKIDPHIPERHHDAIEFPDGSYVLVT
                     QLCEGQRATVLQLPVTQAPHAPAHGAAATPTATRLPVG"
     gene            complement(1391..2083)
                     /gene="lexA"
                     /locus_tag="VM910_29010"
     CDS             complement(1391..2083)
                     /gene="lexA"
                     /locus_tag="VM910_29010"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVI66585.1"
                     /translation="MLTRKQYELLRFINERLKEAGVPPSFDEMKDALDLRSKSGIHRL
                     ITALEERGFIRRLPNRARAIEVIKLPELSPGGGSRRGFTPSVIEGNLGKGRGASADDG
                     ERPVAVPVMGRIAAGTPIEALQTRSHTISLPPDMLGTGEHYALEVRGDSMVDAGILDG
                     DMALIQRNDTAETGDIVVALIDDEEATLKRFRRRGASIALEPANTSYEVRILPPNRVK
                     IQGKLIGLYRKY"
     gene            complement(2259..>3273)
                     /locus_tag="VM910_29015"
     CDS             complement(2259..>3273)
                     /locus_tag="VM910_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HVI66586.1"
                     /translation="NARLKVIGEVAAGRPFDRKLGPGEAARIFTGGVIPDGADAVVIQ
                     EDTVVDGDGITITEAAVAGRHIRPAGIDFHEGDVQLASGRRLSDRDLSLAASMNYPKL
                     PVRRRPRVALLATGDELVMPGATPGPGQIVYSNGYALRALAQAEGAETIDLGVAADTL
                     AATTAGIRRAREVDADILVTTGGASVGDHDLVKTALEAEGVAIAFWRIAMRPGKPMMH
                     GRLGAMRVVGVPGNPVSAYVCTMLFVVPLIRALSGRSDVHPVREDALLGCDVAANDVR
                     EDYLRARLEKRDDGVLVATPVNHQDSSLLANLAAAQALVVRAPFAPGARTGSPCEILR
                     LPL"
BASE COUNT          584 a         1003 c         1065 g          621 t
ORIGIN      
        1 atccggaagg accgcagcat tggcattgcg gacctgctgg ctcatggcct gtggcgcccg
       61 gcggacgtaa tgacgttcgc cggctgccgc gacttggacg ctcgcagcgg cgatcagcgc
      121 agcgccgata agagtaagaa cagtcctgcg catggttgtc tcctcatggt tcacacgttc
      181 tgcacgcgcg gcgggatgcg aaaggtgctg cgcgtttggg tgtcacgcat gtaggcttgc
      241 gcggtcggtt tgataatccg acaagggcgc gaaggacttt caaacaggat tgaacaatct
      301 gggcctgttg aaactaattg tacttagctc tctgcagggg gcgacgcggt cgagggcagt
      361 tcgaaagcac ccagacggaa cttctcaccg tgaaattcac ctgccccgtc tgtgttggaa
      421 ggcctctgga ccagtgtaaa aacggcgtcc ggaaattgct tccagatcgc aaggtcttct
      481 gccgtgacgg tgagtcagcc gaacgtgaac atacaccggc ccggttcggc gatgccgccg
      541 gccttttgcc aactgatctt gttcaccacc ggttgcgctt tcgggtggca acggccccgc
      601 cggccacttg accggcggga ccgtccgcgc agcgtgaaag ctttagccga ccgggagcct
      661 cgttgccgtc ggcgtcgcag ccgcgccatg cgcgggcgcg tgcggagcct gggtgaccgg
      721 caactgcaac accgttgccc gctgtccctc acaaagctgc gtcacgagca cataacttcc
      781 atcaggaaat tcgatcgcgt cgtgatggcg ttccgggatg tggggatcga tcttgccgaa
      841 cttgccgact ttcgaattga tggtccgggt ccagatccat cgattgtcgt atttgacgtc
      901 atcggcaaag gccagctcgg ttcctggcat caagcagaca gcgatgctta gatcgttttc
      961 ggatgcgaag ccgcgggtcg aagtgccacg gaacgtcgtg gtcaccagcg tctctcccac
     1021 ctgggcgggc cgggttgcca cggcgtgcag actgtagtca cacatcggat tgctcctcgt
     1081 tcgagatagc caacccgcgc actctcttaa ttttggaacc gattctatcg cgcaaacgct
     1141 tcgcgtctgt cgcgaaggat catgctcccg gaggcgcagg cctctatcag agtggacagt
     1201 agattgtgag cctttgcccg cttgtaggca attgtgcggc ccgcattttt gggcacgatc
     1261 acaagcgttt gagaatgcgc tgcactggcg cgccacgctt gccggttccg tcggaagatc
     1321 ggagacggcg cataaaaata cagcagagtg ccgcgggctg ggcccgcggc acgatagaga
     1381 ggctggttgc tcagtacttg cggtacaaac cgattagctt gccctgaatc ttgacccggt
     1441 tcggcggcag gatgcggact tcgtaagatg tattggcggg ttcgagcgcg atcgaggcgc
     1501 cgcgccggcg aaagcgcttc aatgtcgcct cttcatcgtc gatcaaggcg accacgatgt
     1561 caccggtctc ggcggtgtcg ttgcgctgaa tcagcgccat gtcgccgtcg aggatgccgg
     1621 catcgaccat ggaatccccg cgaacctcga gcgcatagtg ctcgccggta ccgagcatgt
     1681 ccggtggcaa gctgatggtg tgactgcgcg tctgcagcgc ttcgatcggc gtgccggctg
     1741 cgatccggcc catgaccggc accgcgaccg gacgctcgcc gtcatccgcg cttgcaccgc
     1801 ggcccttgcc cagattgcct tcgatgacgg agggcgtgaa gccgcgccgg ctgcctccgc
     1861 caggcgaaag ctccggcagc ttgatcacct cgattgcacg cgcgcggttg ggcaggcggc
     1921 ggatgaagcc gcgctcctcg agcgccgtga tgaggcggtg aatgccggac ttcgagcgca
     1981 gatccagcgc gtccttcatt tcgtcgaagg agggcggcac ccccgcctct ttgagccgtt
     2041 cattgatgaa acgcagaagt tcgtactgtt ttcgcgtgag catctcgagc agtcccccgg
     2101 ttgctggtgt cgttcgattc tgagacctgc gggagcggcg ctcccggtac tagccctcga
     2161 aacaaatcat gaacggacac tatatgttcg atatgtgttc cgcaaccact taatttcagg
     2221 tgaatgccag taacttcacg agatggggcg cggaaccgtt agagaggcag ccgcaagatc
     2281 tcgcagggcg aaccggtcct ggctcccggc gcaaacggcg cgcgcacgac aagtgcttgt
     2341 gccgcagcaa gattcgccag caaagagctg tcctggtgat tgacgggggt cgcgaccagc
     2401 acgccgtcgt cgcgcttttc cagacgcgcg cgaaggtagt cttcgcggac atcgttggcg
     2461 gcgacatcgc agccgagcag cgcatcttcg cgcaccggat ggacgtcact gcggccagac
     2521 agcgcccgaa tcaggggtac gacaaacagc atcgtgcaaa cgtaggcgga tacgggattg
     2581 ccggggacgc cgacgacgcg catcgcgccg agccgcccgt gcatcatcgg cttgcccggt
     2641 cgcatcgcga tccgccagaa ggcgatggcg acgccttcgg cctcgagggc ggttttgaca
     2701 agatcatggt cgccgaccga cgcgccgccg gtggtcacga ggatgtccgc gtcgacctcc
     2761 cgggcgcggc ggatgccagc cgtggtggct gcaagcgtgt cggcggcaac gccgagatcg
     2821 atggtttccg cgccttccgc ctgggccaag gcccgcaagg catagccgtt cgaatagacg
     2881 atctggccgg gcccaggtgt cgcgccgggc atcacgagct cgtcgccggt cgcaagcagt
     2941 gcgaccctcg gccggcgccg caccggcagt ttcgggtagt tcatgctggc tgcgagcgag
     3001 aggtcgcgat cgctgaggcg gcggccggag gcgagctgca cgtcgccttc gtgaaaatcg
     3061 atgccggcgg ggcggatgtg acgtcccgcc accgcggctt cggtaattgt gatgccgtcg
     3121 ccatcgacca cggtatcttc ctggatcacg acggcgtccg caccgtctgg aatgacgccg
     3181 ccggtgaaga tgcgcgcggc ttcgcccggg ccgagcttgc ggtcgaacgg acggccggcg
     3241 gcgacttcgc cgatcacctt cagccgcgca ttg
//
LOCUS       DATLJT010001266         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1937803,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001266 DATLJT010000000
VERSION     DATLJT010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937803"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VM910_29020"
     CDS             complement(<1..107)
                     /locus_tag="VM910_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HVI66587.1"
                     /translation="MSATPIKVLVVDDEPPIRKLLRMGLTTQGYETLEAP"
     gene            complement(104..>2123)
                     /locus_tag="VM910_29025"
     CDS             complement(104..>2123)
                     /locus_tag="VM910_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVI66588.1"
                     /translation="VDEQLLTHMQANAIAGPWAAGERILVCVSEDPRAAGLVRYTKRV
                     ADRVHAPWTAISIETRRSMQLTDEQRDQLAETMRLAEALGGEALTIPGVGRSIADDII
                     QFAQANNVTQIIIGKSSRSRWFEIMRGSVVHDLVRRSGNITVNVIAGDELPSGPAMST
                     AVQTAARPEPFNPRPYLMALVIVAAGLVVAEAIQPWFGIENVDLVLLTAVVAVAVRYG
                     LWPSLLAAVAASLCYNFFFLPPIYTFTITDPTNIAAFFLFMLVAMLVSNVAARVRSQA
                     DTAIGRIRMTEQLYAFSRKLAGTATLDDVLWATAYQTALMLKVRVVLLLPEDGVLTVK
                     AGYPPEDQLDKADLAAANWAWSNDRPAGRGSDTLPGAKRLFLPMRTGRGSIGVIGIDN
                     DRTGPLLTPDQRRLLDALVDQGALAIERVLLVEDMDKVKRTVESERLRSALLTSISHD
                     LKTPLASVLGAASTMRDLEYGLSDTEKRDLLATVIDESERLNRFIANLLDMTKLESGA
                     IVPNTALHDIGEIVGSALRRGSKILSRHRISLELDADLPMLELDAVLFEQVIFNLLDN
                     AAKYSPSETTISIRSSRDANSVLLQIADEGEGIPPAELESIFDKFYRVQKADHVRPGT
                     GLGLAISRGFVEAMHGRISAANRTDRSGAVLTIRLPIPASAGKLDTAA"
BASE COUNT          388 a          672 c          689 g          374 t
ORIGIN      
        1 ggcgcctcca gcgtttcgta gccctgcgtg gtcaatccca tccgcagcaa cttgcggatc
       61 gggggttcgt cgtcgacaac gagaaccttg attggcgtcg cgctcatgca gcggtgtcca
      121 gttttcccgc cgaggccgga atcggcaggc ggatcgtgag caccgcacca ctgcgatcgg
      181 tacggttggc ggccgaaatc cggccatgca tcgcctcgac gaagccccgt gagatcgcaa
      241 gtccgagacc ggtgccgggc cggacatggt cggccttctg cacccggtag aacttgtcga
      301 agatgctttc gagttctgcc ggcggaatgc cctcgccttc gtcggcaatc tgcagcaaca
      361 ccgaattggc gtcgcgcgag cttcggatcg agatggtcgt ctccgatggc gaatatttgg
      421 cggcattgtc gagcaggttg aagatcacct gctcgaacag aacggcatcg agctcgagca
      481 tcggcaaatc cgcgtcgagc tcgagcgaga tcctgtgccg tgaaagaatc ttgctgcctc
      541 gcctcagcgc gctgccgacg atctcgccga tgtcgtgcag cgcagtgttc ggcacgatag
      601 cgccggactc cagcttggtc atgtcaagca ggttggcgat gaaacggttg agccgctcgg
      661 actcgtcgat cacggtcgcg agcaggtcac gcttttcggt atccgacagg ccatactcga
      721 gatcgcgcat agtagaggcc gcgcccagca cggacgccag cggcgttttg aggtcgtgcg
      781 aaatcgaggt aagcagcgcc gagcgcagcc gctcagattc cacggtgcgc ttgaccttgt
      841 ccatgtcctc gaccagcagc acccgctcga tggcgagcgc cccctggtcg accagcgcgt
      901 ccagcaagcg gcgctggtcc ggcgtcagca acgggcccgt tcggtcattg tcgatgccga
      961 taaccccgat cgaaccgcgc ccggtccgca ttggcagaaa cagccgcttt gcgcccggca
     1021 gcgtgtccga gccgcggccc gcggggcggt cgttgctcca cgcccagttc gccgctgcca
     1081 ggtctgcctt atcgagttga tcctcgggcg ggtagcccgc cttcaccgtc aacacgccgt
     1141 cttcgggcaa cagcaaaacc acgcgcacct tgagcatcag cgcggtctgg taggcggtcg
     1201 cccacagcac gtcgtcgagc gtcgcagtgc ccgcaagctt gcggctgaag gcgtagagtt
     1261 gttccgtcat cctgatgcgc ccgatcgcgg tgtcggcctg cgagcgcacg cgcgcggcca
     1321 cgttggagac caacatcgcg acaagcatga aaaggaagaa ggccgcaata ttggtcggat
     1381 cggtgatcgt gaaggtatag atcggcggca ggaagaagaa attatagcag agggacgccg
     1441 caacagcggc caacagtgaa ggccacaggc catagcgcac cgcgaccgcc accactgctg
     1501 tcagcagcac cagatcgaca ttctcgatgc cgaaccacgg ctggatagct tcggcaacaa
     1561 ccagacccgc cgccacgatc acaagcgcca tgagataggg gcgcggattg aacggctcgg
     1621 gccgcgcggc ggtttgcacc gcggtgctca tggctggtcc cgatggcaat tcgtcgccgg
     1681 ctatgacgtt gacggtgata ttgccggacc gtcgcacgag atcgtgtacc accgagcctc
     1741 gcatgatctc gaaccagcgc gagcgcgagg atttgccgat gatgatctgc gtgacgttgt
     1801 tggcctgagc gaactgaatg atgtcgtcgg caatactgcg gccgacgccg gggatggtga
     1861 gggcctcgcc gccaagcgcc tcggccagac gcatggtctc ggcaagctgg tcgcgctgct
     1921 cgtcggtcag ttgcatgctg cgccgggtct cgatcgagat cgcggtccag ggcgcgtgca
     1981 cacggtcggc gacccgtttg gtgtagcgca caagccccgc cgcgcgcggg tcttcgctga
     2041 cgcagaccag gatgcgctcg cccgcggccc agggtccggc aatcgcgttg gcctgcatgt
     2101 gggtgagcaa ttgctcgtcg acg
//
LOCUS       DATLJT010001267         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2857110,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001267 DATLJT010000000
VERSION     DATLJT010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857110"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1006
                     /locus_tag="VM910_29030"
     CDS             131..1006
                     /locus_tag="VM910_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66589.1"
                     /translation="MTEQATSQLRRRMIEDMTIRKFVAKTQHDYVQRVKDFASFLGRS
                     PASASKEDIRRFQLHLASNGAGTPKMNATAAALRFFFNVTLDRHELAAQASFVHEPRK
                     LPVVLSPDEVRRMLEAAPGLKYKAALSIAYGAGLRVSEVVSLKVSDIDSERMLIRVEQ
                     GKGRKDRNAMLSPVLLELLREWWKVCRSRGWLFPGGRNPVQPMTTRQLTRACHTAAQA
                     AEIKKRVTPHTLRHSFATHLLEQGIDVRVIQVLLGHAKLDTTALYTCVATKTIREVVS
                     PLDHLVKVFKARSPG"
     gene            1019..>2117
                     /locus_tag="VM910_29035"
     CDS             1019..>2117
                     /locus_tag="VM910_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HVI66590.1"
                     /translation="MPRPALEVANIFRGHGPAWRIANAGHVSLDQFKVMTAIENCRTA
                     ALGGHVLRCENCAHTLIAYNSCRNRHCPKCQGAAAKEWLAEREAELLPVPYFHVVFSL
                     PSQIADIAYQNKSVIYDLLFKASSETMLTIAADPSHLGARIGFLSVLHTWGSALNHHP
                     HVHMIVPGGGFSLDGKRWISCRPHYLFPVQVLSALFRRLLLEKLVVAHAAGELQLFGK
                     HAQLADAKVFAAYLKPLRNSKWVVYCKDPFGGPEQVLRYLARYTHRVAISNRRLVAHD
                     EKGVTFKWKDYRLEGPERYKIMTLATDEFIRRFLMHVLPAGFHRIRYYGLLASGRRAE
                     NIARARELLTPPMVPIEAIKALSNAVSQQTAK"
BASE COUNT          428 a          683 c          607 g          399 t
ORIGIN      
        1 ggactaaacc gctcgcgcgg taaggtgcct gtggcgcagt tcgcgcaatt atcaacagcc
       61 gtagcgggtg aaggcgtcct gtcctgagat cgaggtgttc caagacctca ctcaagacag
      121 gagcacctcc atgaccgagc aggcaacgag ccagctgcga cggcgcatga tcgaagacat
      181 gacgatccgc aagttcgtcg cgaagaccca acacgactac gtgcaaaggg tcaaggactt
      241 cgcttctttt ctcggccgat caccggcgag cgcaagcaag gaagacatac gccgttttca
      301 gttacatctg gcatcgaatg gcgccggtac gcccaagatg aacgccacgg cggcagcgct
      361 gcgtttcttt ttcaacgtaa cgcttgatcg gcacgaactc gccgcacagg cctcgttcgt
      421 gcatgagccg cgcaaactgc ctgtggtgct cagtccagac gaagtgcgac gcatgctgga
      481 ggcggctccc ggccttaaat acaaggcggc actgagcatc gcctatggtg cggggctacg
      541 ggtatccgag gtcgtctcgc tcaaggtctc cgacatcgac agtgagcgca tgctgatccg
      601 ggtcgagcag gggaaaggtc gcaaggaccg caatgccatg ctctccccgg tgctactcga
      661 gctgttgcgc gagtggtgga aggtttgccg atcgcgcgga tggctattcc ccggcggtcg
      721 caatccggtg cagccgatga ccacccggca gctcactcgt gcctgtcaca cggccgcgca
      781 agccgccgag atcaagaagc gcgtcactcc gcacacgctg cggcacagtt ttgcaacgca
      841 tctgttggag caaggcatcg atgtccgcgt gatccaggtc ttgctcggtc acgccaagct
      901 cgacaccacg gccctctaca cgtgcgtggc gaccaaaacg atccgtgagg tggtcagccc
      961 gctcgatcat ctcgtcaaag tcttcaaggc aaggtcaccg ggctgacgcg cggcgcgcgt
     1021 gccgcgcccg gcgttggagg tcgcgaatat cttccgcggc catggtccgg catggcgaat
     1081 tgccaatgcc ggtcatgtga gcctcgacca attcaaggtg atgacggcga tcgagaactg
     1141 ccgcacggcg gcgctcggcg gacatgttct gcgctgcgag aactgcgctc ataccctcat
     1201 cgcctacaat tcctgccgca atcggcattg cccgaagtgc caaggggcgg cagccaagga
     1261 gtggctggcc gagcgcgagg cggagttgtt gccggtgccg tactttcacg tggtgttctc
     1321 gttgcccagc caaatcgcag acatcgccta tcaaaataaa tccgtgatct acgatctgct
     1381 gttcaaggct tcatcggaga cgatgctgac gatcgcggcc gatcccagcc acctcggcgc
     1441 ccgcatcggc ttcctctccg tcctccatac ctggggttcg gcgctcaacc atcatccgca
     1501 tgtccacatg attgtgccgg gtggcggatt ctcgctcgac ggtaaacgtt ggatctcctg
     1561 ccggccccat tacctcttcc cggtacaggt tctctcggcc ctgttccgcc ggttgctcct
     1621 ggagaagctt gtcgtcgcgc acgccgccgg cgaactgcag ctcttcggca agcatgccca
     1681 actcgccgac gcgaaagtct tcgccgccta tctcaagccg ctgcgcaaca gcaaatgggt
     1741 cgtctattgc aaagacccgt tcggcggacc tgagcaagta ctacgctatc tcgcccgcta
     1801 cacccaccgc gtcgccatct ccaaccgccg cttggtcgcc catgatgaga aaggtgtcac
     1861 cttcaaatgg aaggactaca ggctcgaagg tcccgaacga tacaagatca tgacgcttgc
     1921 gacagacgag ttcatccgtc gtttcctgat gcacgtattg cccgcaggct tccatcgcat
     1981 ccgctactac ggcctgcttg ccagcggcag gcgagccgag aatatcgccc gcgcccgcga
     2041 actgctcacg ccgccaatgg ttccgatcga ggcgatcaaa gcactcagca atgccgtcag
     2101 ccaacaaaca gcaaaac
//
LOCUS       DATLJT010001268        11141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3546475,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001268 DATLJT010000000
VERSION     DATLJT010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3546475"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VM910_29040"
     CDS             <1..256
                     /locus_tag="VM910_29040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HVI66591.1"
                     /translation="ASDPVDQSLLHEVLNGQMVFSIEATSAIFGASHLSFDGDLMDFV
                     ASAAGAVIVLIVGSIWRSRKLRESARVASLAAAEAAQRAD"
     gene            complement(268..1770)
                     /gene="ppx"
                     /locus_tag="VM910_29045"
     CDS             complement(268..1770)
                     /gene="ppx"
                     /locus_tag="VM910_29045"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HVI66592.1"
                     /translation="MSRPRKRAPSVAVIDIGSNSVRLVVYERMARSLVSVFNEKTLCG
                     LGREVQSTGLLAPDAVDKALTSLRRFHALCRVQRVGRVYAIATAACRDASNGPDFIAK
                     AERICRVRIEILSGPREAKLSALGVVSGIHKPDGIVGDLGGGSLELIDVRGHQVRGGV
                     TLPLGSLALQDLSHKSLKRAERIVKNELEEIPRLKAGRGRTFYAVGGTWRALARIHIV
                     QSGYPLKVMHGYSITAADALDFVQRLRRLAAANMLDNIEAVADARRPLLTYAALVLEY
                     IIRVAKPKNITFSTFGVREGLLYSMLGESERAKDGLLCAAQSLNELLSRSARHAQELI
                     EWTDRLARVVHLRETEEDRRLRHAACLLSDIAWRVHPDHRGEQTLELITNGNFGSITH
                     QGRAFVALSVFFRYAGLSEENQPPVIIQEQVTPTMLERARVLGAAFRVAHLISAARPG
                     VLSATHFRSNGRKLMLVFEHQMVDLVADRVGSRFKQLARLVGRSGSIVRR"
     gene            complement(1783..3981)
                     /locus_tag="VM910_29050"
     CDS             complement(1783..3981)
                     /locus_tag="VM910_29050"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HVI66593.1"
                     /translation="MESEQAIVIEEKQAAPQTASPAAAGPERFINRELSWLHFNRRVL
                     EESVNPGHPVLERVRFLSISANNLDEFFMVRVAGIKAQVREGIPERSPDGLTPAEQLV
                     LINQTVSKLASDQQAIWRELRHVLSAVGIVLVEGRDVTKAERTWVEDYFLHNIFPLLT
                     PLAIDPAHPFPFIPSLGFTIALQLTRTSDGKPMNALIRMPGKIDRFIRMPAAKDGVVR
                     LLSLEQATGLFINRLFPGYTVNGQGAFRIIRDSEIEIEEEAEDLVRVFETALKRRRRG
                     SVIRLEIDANMPEELRTFVQQALSVADDEVFLVDGVLAVNELSQLTRLDRPDLEFTPY
                     VPRHPERVREHGGDIFAAIRQKDLIVHHPYESFDVVVQFLQQAARDPDVVAIKQTLYR
                     TSNNSPIVRALGEAAEAGKSVTALIELKARFDEEANIRWARDLERAGVQVVYGFLELK
                     THAKLSMVVRREGGSLTTYVHTGTGNYHPVTARIYTDLSFFTSDPAIGRDAARVFNYI
                     TGYAAPSDLEKMAVSPLTLRKRIISHIRDETSHARHGKPAAIWMKMNALVDPEIIDAL
                     YEASQAGVSVELVVRGVCCLRPGLPGLSENIRVKSVIGRFLEHGRIYCFGMGQGLPGS
                     KAAVYISSADMMPRNLDRRVEVLCPLQNPTVHQQVLEQIMVANLKDNEQSWQLLPDGS
                     STRMKAAKGDEPFNLHNYFMTNPSLSGRGKSLKESSPRRLTRRTERHHSS"
     gene            complement(4113..4790)
                     /locus_tag="VM910_29055"
     CDS             complement(4113..4790)
                     /locus_tag="VM910_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HVI66594.1"
                     /translation="MAGRVQPRQLAFALPHAESLTRDNFQEGPANAAALALIDSWPEW
                     PSRTMLLVGPKGSGKSHLATIWAEQAGARSTTAHALTAANVPAALATGALVVEDLNEA
                     TFDERALFHLLNLAREDQAHVLITARVAPTALEIELRDLRSRLRALPVVTIQPPDDQL
                     FRALIIKFCADRQLPVDEALVSYLAARIERSYVAARQAVEFLDSEALRLGRPVTRALA
                     AEVLRNS"
     gene            complement(4894..5439)
                     /locus_tag="VM910_29060"
     CDS             complement(4894..5439)
                     /locus_tag="VM910_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVI66595.1"
                     /translation="MSIPNSIPNLITLGRILLVPIIVWAIASNQMEFAFAVFIVAGVS
                     DAVDGFLAKRFNMTSELGALLDPLADKALLVSIYIALGIWGAVPRWIVILVVSRDLMI
                     VAAVIVSWLFDKPVAMKPSMLSKLNTVAQVAFAALVLGSLGFDFKPMPYDLILMGLVT
                     VFTLSSVSLYLVEWVRHMSTI"
     gene            5590..6663
                     /gene="purM"
                     /locus_tag="VM910_29065"
     CDS             5590..6663
                     /gene="purM"
                     /locus_tag="VM910_29065"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HVI66596.1"
                     /translation="MAERKNGLTYADSGVDIDAGNRLVDLIKPMVRATARAGSDAEIG
                     GFGGLFDPKAAGFKDPVLVAATDGVGTKVKIAIETGLHGSIGIDLVAMSVNDLVVQGA
                     EPLFFLDYFACGKLDPEATAAVIAGIAEGCRESGCALIGGETAEMPGLYKDGDYDLAG
                     FAVGAAERGTLLPRPDIAIGDAIIGLGSSGLHSNGFSLVRKIVAQSGLPFEAPAPFSP
                     VMTLAGALLAPTRLYVKSCLRAIRETGAVKGLAHITGGGFTDNIPRVLPKHLGVGIDL
                     ARLPVLPVFKWLAEQGGLAESELLRTFNCGIGMIAIVRPDAIKEVTEVLTSSGESVAM
                     LGTVLPAEGERRVVYNGHLDLRR"
     gene            6668..7339
                     /gene="purN"
                     /locus_tag="VM910_29070"
     CDS             6668..7339
                     /gene="purN"
                     /locus_tag="VM910_29070"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HVI66597.1"
                     /translation="MKRRVAILISGRGSNMAALIKAAAAADYPAEIVLVISNRADAGG
                     LERASASGIATVVIESNPFGKDRAAFEAVLQAALDQYRIELVCLGGFMRLFTADFARR
                     WYGRMLNIHPSLLPSFPGLDPHGQALRAGVKISGATVHFVIPETDAGPILMQGAVAVS
                     DHDTAETLSERILEVEHRIYPDALRLLATGAIELDGDICKTPDSAGSGNFLISPVASA
                     DGTSA"
     gene            complement(7430..7633)
                     /locus_tag="VM910_29075"
     CDS             complement(7430..7633)
                     /locus_tag="VM910_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVI66598.1"
                     /translation="MPKGTVKWFNPTKGYGFIKPQAGDKDVFVHISAVERAGLATLNE
                     NQVVEYDLVENRGKTSAENLKVS"
     gene            8066..9112
                     /locus_tag="VM910_29080"
     CDS             8066..9112
                     /locus_tag="VM910_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HVI66599.1"
                     /translation="MLAAIGSSPVFGQGAFVPGPAPTSVPTPIDINSEIASGAQVLDL
                     GGSFLERLGNQAAGGFGRALRNNPGGGGASEAVEGPRFRSWGEVYGLSLTTGPQADFL
                     GDRRQTLGGVVGFGAGIAPGVNVGFSVDQSHTAIDVPLAIQSATIDLTQLGFNASVDK
                     GPWTWAIALVYGFGNVGSSRDTGQGLALAGYGTQLYGALTELNYYWDLQQSRVVPKLD
                     FEYVRATTGAFQETGGLNPVSATGATLERARVLVGAEIGHYWIFDRNVLDLSAYGKFV
                     DNFSQSFSSVVVSLGPQAIAVQGILESQYGADTGASASLSLGNMTRIYLNYDGKFRSA
                     LQSHQGTLGFEARW"
     gene            complement(9136..10182)
                     /locus_tag="VM910_29085"
     CDS             complement(9136..10182)
                     /locus_tag="VM910_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI66600.1"
                     /translation="MQQLLHRGAATMQHRTDEVLDASWDDLKSFLACARCKSFRNAAE
                     ELQVTSTTLMRRIDRLEERIGCRLFLRDQSGLTLSEEGAAMLADVTEMERHAFNVFRR
                     AAKSANDTAGTVRVAVTEGPGNFWILPRLIDFQKTYTKITVDLRCAMEQADVARLESD
                     IAIQLEPPTNPELIVAKLGRLHIYPFVSKGYQKLYGTPSSLSELRNHRIIKQNAPQVD
                     DTAYARVLGLKSLEGVVGIKTNSSIGVLYAVERGAGIGFLPTSSIALGAPLVAVDMDG
                     FSHHADLWLTYHREFRNSDRHKIVIDWLKSIFDPKTYACFRDEFIHPNALVPMMATAR
                     ESFGLSGYVAASPG"
     gene            10236..10862
                     /locus_tag="VM910_29090"
     CDS             10236..10862
                     /locus_tag="VM910_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66601.1"
                     /translation="MRDPIKERGPLAERHDFDSLPTDGDITVKCARYVELSQLADMAH
                     RLVPGVRMTADILGKYLAWDPECILTFSRQERLLGAIAFLYLNDRGHDALLLDEICLT
                     QPEIEFLAARGEEVSATYIWACAITGRGISGYGKVAEYLRRPRYVNADVFAQPSTKAG
                     RDWLVATCFKQTQSFQPDLWCYERSWNRLSIPVPASGVLVRSYADARH"
     gene            10849..>11141
                     /locus_tag="VM910_29095"
     CDS             10849..>11141
                     /locus_tag="VM910_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI66602.1"
                     /translation="MHGTRPSALQGADQRAITVRIARDPNDLMLVTAIRSAVYLAEQD
                     CPLEEEFDGNDLVAAHFLGFVGKEPAGCLRVRFFGEFAKVERLAVRHQYRRTRV"
BASE COUNT         2091 a         3394 c         3512 g         2144 t
ORIGIN      
        1 cgcgagcgat ccggtcgatc agtcgctgct tcacgaggtg ctcaacggcc agatggtctt
       61 cagtatcgaa gcgacctcgg cgatcttcgg cgcatcgcat ctcagcttcg acggcgatct
      121 gatggatttc gtcgcctcgg cggcgggcgc ggttatcgtg ctgatcgtgg gttcgatctg
      181 gcgatcgcgc aagctgcggg aatccgcacg cgtcgcgagc ctggccgccg ccgaagcggc
      241 ccaacgcgcc gattagagga tcacgcgtta gcgcctgacg atcgaccccg accggccgac
      301 aagccttgcg agctgcttga agcggctgcc gacgcggtcg gcgaccagat ccaccatctg
      361 atgctcgaat accagcatca gctttcgccc gttgctgcgg aaatgtgtcg ccgacagcac
      421 gcccggccgc gccgccgaga tcaaatgggc gacccggaac gctgcgccga gcacgcgagc
      481 gcgctccagc atcgtcggcg tcacctgctc ctggatgatg acgggcggct ggttttcctc
      541 gcttaagcct gcgtagcgga agaacactga cagcgcgaca aaggcgcggc cctgatgcgt
      601 gatcgagccg aaattgccgt tggtgataag ctcgagcgtc tgctcaccgc ggtgatcggg
      661 gtgcacccgc caggcgatgt ccgagagcag gcaggcggca tggcggagcc ttcgatcctc
      721 ctcggtctcg cgcagatgca cgacacgcgc aagccgatcg gtccattcga tcaattcctg
      781 ggcgtggcgc gccgagcgcg acagcaattc gttcagtgac tgcgcggcgc aaagcaaacc
      841 gtccttggcg cgctcgctct cccccaacat cgaatacagg agaccttcgc gcaccccgaa
      901 ggtcgaaaac gtaatgtttt tcggtttggc gacccggatg atgtattcga ggaccagcgc
      961 cgcgtaggtc agtagcggac gccgtgcatc cgcgacggcc tcgatattgt ccagcatgtt
     1021 ggcagcagcc agccgccgca gcctttgcac gaaatcaagc gcgtcggcag ccgtgatcga
     1081 atagccgtgc atgactttca gcggatagcc gctctggacg atgtgaatgc gcgccaatgc
     1141 gcgccaggtc ccgccgacgg catagaaggt gcggccgcga ccagccttca gccgcgggat
     1201 ctcttccaat tcgttcttga cgatgcgctc cgctcgcttc agcgatttgt gcgaaaggtc
     1261 ctgcagcgcc aggctgccga gcggcagcgt cacgccgcca cgcacctgat ggccccgcac
     1321 atcgatcaac tccagcgaac cgccgccgag gtcgccaacg atgccgtcgg gcttgtgaat
     1381 gcccgagacg acaccgagcg cagagagctt ggcttcgcgc gggccggaca ggatttcgat
     1441 gcgaacacgg cagatgcgct cggcttttgc gatgaagtcc ggcccgttgc tggcatcccg
     1501 gcaagcagcg gtggcgatgg cgtagacacg gccgacccgc tgcaccctgc acagcgcatg
     1561 aaaccgtctc agcgacgtca aggccttgtc gacggcatcg ggtgccaaaa gcccggtgct
     1621 ttgcacctca cggcccaacc cgcacaacgt cttctcgttg aacacggaaa cgaggctccg
     1681 cgccatccgc tcatagacga cgagacgaac cgagttcgag ccgatgtcga tgaccgcgac
     1741 gctcggtgca cgctttcgcg gccggctcac cggcagagcc ccctaagacg aatgatgacg
     1801 ttcggtacgg cgcgtgagac ggcgcggtga ggattccttg agcgactttc cacggcctga
     1861 gagactcgga ttcgtcatga aatagttatg aaggttaaaa ggctcgtcgc cttttgcggc
     1921 cttcatacgc gtagacgacc cgtccggcaa caattgccag ctctgctcgt tgtccttcag
     1981 gttggcgacc atgatctgtt cgagaacctg ctgatgcacc gtaggatttt gcagcggaca
     2041 cagcacctcg acgcggcggt cgagattgcg cggcatcatg tcggcggagg agatatacac
     2101 agctgctttc gagccgggta agccctgtcc catgccaaaa cagtagattc ggccgtgttc
     2161 gaggaagcgg ccgatcactg atttgacgcg gatgttctcg gaaagtccag gcagtccggg
     2221 ccgcaggcag cagacaccgc gtaccaccag ttcgaccgag acgccggcct gggacgcctc
     2281 gtatagcgcg tcgatgatct cggggtcgac cagtgcattc atcttcatcc agatcgcggc
     2341 gggcttgccg tggcgggcat ggctggtttc gtcacgaatg tgcgaaatga tccgcttgcg
     2401 cagcgtgagc ggcgatactg ccatcttctc cagatcgctc ggcgcagcat aaccggtgat
     2461 ataattgaat acccgtgccg catcgcggcc gatcgccgga tcggatgtga agaacgaaag
     2521 gtcggtgtaa atgcgcgcgg tgaccgggtg gtaattgccc gtcccggtgt ggacataggt
     2581 ggtgagactg ccgccctcgc ggcgtacgac catcgacagc ttggcgtggg tcttcaattc
     2641 aaggaagcca tacaccacct gcacgccggc gcgctcgagg tcgcgcgccc agcggatgtt
     2701 ggcttcctcg tcgaagcgcg ccttcagctc gatgagggcg gtcaccgact tgccggcctc
     2761 cgcggcttcg cccagcgcac gcacgatcgg cgaattgttg gaggtgcggt agagcgtctg
     2821 tttgatggcg acaacgtcgg ggtcgcgcgc ggcctgctgg aggaattgca ccacgacatc
     2881 gaaggattcg taggggtgat ggacgatcag gtctttctgc cggatcgctg cgaagatatc
     2941 gccgccatgt tcgcgcaccc gctccgggtg gcggggcaca tacggggtaa actcaagatc
     3001 aggccggtcg agccgggtca gttgtgacag ctcgttcacg gccagcacac catcgacaag
     3061 gaaaacctcg tcgtcagcga ccgatagcgc ctgctgcacg aaggtgcgca gctcctccgg
     3121 catgttggca tcgatttcaa gccggatcac cgatccgcgg cgccgccgct tcagcgcggt
     3181 ctcgaaaacc cgcaccagat cttccgcctc ttcctcgatt tcgatttcgg aatcccggat
     3241 gatccggaat gcgccttggc cgttcaccgt gtagccggga aacagcctgt tgatgaacag
     3301 gccggttgcc tgctcgagcg atagcagccg caccacgccg tccttcgccg cgggcatgcg
     3361 gatgaagcgg tcgatcttgc cgggcatgcg gatcaacgcg ttcatcggtt tgccgtcgga
     3421 cgtgcgggtc aattgcaacg cgatggtgaa cccgaggctt gggatgaacg gaaagggatg
     3481 cgcgggatca atcgcaagag gagtcaacaa cggaaagatg ttgtggagga agtaatcctc
     3541 gacccaggtt cgctcggcct tggtgacgtc acggccctcg acgagcacga ttccgaccgc
     3601 ggaaagcacg tggcgcagct cgcgccagat tgcctgctgg tcgctggcga gttttgaaac
     3661 tgtctggttg atcaggacga gctgttcggc gggcgttaac ccgtcggggc tgcgttcggg
     3721 gatgccttcg cggacctgcg ccttgatgcc ggcgacgcgg accatgaaga actcgtcaag
     3781 gttatttgcg gaaatcgaca ggaaccgcac ccgctcgagc acggggtggc cgggattgac
     3841 ggattcctcg aggacccggc ggttgaaatg cagccaggaa agctcgcggt taataaagcg
     3901 ctcagggccg gcggcagccg gcgaagcggt ctgaggggcg gcctgtttct cttcaattac
     3961 aatggcttgc tcagattcca tgagagttcg atccaaacgc ggcttgaaat ctcatccgct
     4021 tttagcctgg tgcggcgcaa actgtgtgac accggggaga ttgtccatgc catgatgtcg
     4081 cgagccggca ggacgtcaag acgcgggttc gttcaagaat tgcgtaggac ttcggcggcc
     4141 agcgcccgcg tcacgggccg tcccagccgc agggcctcgc tatcgaggaa ttcgactgcc
     4201 tgccgcgccg caacataaga tcgctcgatc cgggcggcga gatagctgac cagggcctca
     4261 tcgaccggga gctggcgatc agcgcaaaac tttatgatca gtgcccggaa tagctgatcg
     4321 tcgggcggct ggattgtaac caccggcagc gcgcgcagcc gcgagcgcag gtcgcgcaac
     4381 tcgatctcga gcgcggtggg ggcgacgcgc gcagtgatca gtacatgggc ctgatcctcg
     4441 cgcgcaaggt tcagaaggtg aaacagcgcg cgttcgtcga atgtcgcctc gttcaaatcc
     4501 tccacgacga gcgcgccggt ggctagcgcg gcgggcacat tcgcggcggt cagggcgtgc
     4561 gcggtagtag aacgcgcacc cgcctgttca gcccagatcg tcgccaggtg gcttttgccc
     4621 gacccttttg gcccgaccag cagcatggtc cggctcggcc attcaggcca actgtcgatc
     4681 agcgcgaggg cggcggcgtt ggcaggtccc tcctggaaat tgtcgcgcgt caggctttcc
     4741 gcgtgtggca gtgcgaacgc caattgacgg ggttgaacgc gacctgccac gcgaattgct
     4801 ccatgccggg aacgatccgg cgccttccaa cggtcttggg ctagtggtcc aattccaaca
     4861 tacgcatccc atccgggatg cgtatttgcg aaactagatc gtgctcatgt gccgtaccca
     4921 ttccacgaga taaagggata cggaggacag cgtaaagacc gtaacaagcc ccatcaggat
     4981 cagatcgtag ggcatcggct tgaagtcgaa gccgagcgag cccagcacca atgccgcgaa
     5041 cgccacctgg gccaccgtat tgagcttcga caacatcgac ggcttcattg cgaccggctt
     5101 gtcgaacaac cacgacacga tcacggccgc gacgatcatg aggtcgcgcg acaccaccag
     5161 gatcacgatc cagcgcggga cggcgcccca gattccaagc gcgatataga tcgagacgag
     5221 cagcgccttg tctgccagcg gatcaagcag tgcgcccagc tcgctcgtca tgttgaagcg
     5281 cttggccaga aacccgtcga ccgcatcgct gacgccggcg acgatgaaca cggcaaaagc
     5341 gaattccatc tggttggagg cgatcgccca gacgatgatc gggaccaaaa ggatccggcc
     5401 cagcgtgatg aggttgggga tgctgttcgg aatgctcacc tggcgcttcc cggcgcgaaa
     5461 aggtccggcc ccgtttggcg gttgagccgg ttgttatcta catagtacat ggctcgcggt
     5521 cttgcgagcc attgcacggc ggcgaaatcc gacgtaacca gccgaaatca ttccggaact
     5581 gtgccgccga tggccgaacg caaaaacggg ctcacctacg ccgattcggg cgtcgatatc
     5641 gacgcaggca atcgcctggt cgatctcatc aagccgatgg ttcgtgccac ggcgcgcgcg
     5701 gggagtgacg ccgagatcgg cggcttcggc ggactgttcg atccgaaggc cgctggcttc
     5761 aaggacccgg tgctggttgc cgcaaccgac ggcgtcggca ccaaggtgaa gatcgcaatc
     5821 gagaccggat tacatggcag catcggcatc gatctcgttg cgatgtcggt caatgatctc
     5881 gtcgtgcagg gcgcggagcc cttgttcttc ctggactact ttgcctgcgg caagctggat
     5941 ccggaggcca ccgccgccgt catcgcgggc atcgccgagg gctgccggga gtccggttgt
     6001 gctctgatcg gtggcgagac agcggagatg cccgggctct acaaggacgg cgactacgat
     6061 cttgccggtt ttgcggtcgg cgcggccgag cgcggaacgc tgttgccgcg acccgacatc
     6121 gcgattggcg acgccatcat cgggctcggg tcgtcgggcc tccactccaa cggcttttcg
     6181 ctggttcgca aaatcgtcgc gcaatccggt ctgcccttcg aagcgccagc gccgttttcg
     6241 ccggtcatga cgctggcagg cgcgctgctt gcgccgacgc ggctctatgt gaagtcctgc
     6301 ctgcgcgcga tcagggagac tggcgcggtg aaggggctcg cgcacattac cggcggcggc
     6361 ttcaccgaca acattccgcg cgtcctgccg aaacatctcg gcgtcgggat cgatctcgcg
     6421 cgtctgccgg tgctgccggt gttcaaatgg ctggccgagc agggtggtct tgcggagagc
     6481 gagttgctgc gtacgttcaa ttgcggcatc ggcatgatcg cgatcgtcag gccggatgcg
     6541 atcaaggagg tgactgaagt gcttacctcg agcggcgaga gcgtggcgat gctgggcacg
     6601 gtgctcccgg ccgagggcga gcgtcgcgtc gtctataacg gccatcttga tctcaggcgg
     6661 tgagtgcatg aaacgccgcg tcgccatcct gatttccggg cgcggttcga acatggccgc
     6721 gctgatcaag gccgctgctg cggccgatta tccggcggaa attgtcctcg taatctcgaa
     6781 ccgcgcggac gcgggcggtc tcgagagggc gagcgcaagc ggcattgcga ccgtggtcat
     6841 cgagagcaac ccgttcggaa aggatcgtgc tgccttcgag gcggtgttac aggcggcgct
     6901 tgatcagtat cgcatcgagt tggtttgcct cggcggcttc atgcgccttt tcacggccga
     6961 tttcgcgcgg cgctggtatg ggcggatgct gaacatccat ccctcgctgc tgccatcctt
     7021 tccgggactc gatccgcacg ggcaggcgct gcgcgccggc gtgaagattt ccggcgccac
     7081 cgtccatttt gtcatccccg agaccgacgc cggtccgatc ctgatgcagg gcgcggtcgc
     7141 cgtcagcgat cacgataccg ccgagacgct ttcggagcgc attctggagg tcgagcatcg
     7201 catctacccc gatgccttgc ggctgctcgc caccggcgcc atcgagctgg acggagatat
     7261 ctgcaagacg ccggacagtg ccggctccgg caatttcctg atctcgccgg ttgccagcgc
     7321 cgacgggaca tcggcctgag cgctcgcagt ctacccccgg ccttgaaaca aaaatccccc
     7381 ggcaattgcc gggggtcact tgtcatggca ggcgcggatc ccgcgaaagt caggagacct
     7441 tcaggttttc cgcggaagtc ttgccgcgat tttccacgag gtcatattcc acgacctggt
     7501 tttcgttgag ggtggcgagt cccgcgcgct cgacggcgga gatgtgaacg aacacgtcct
     7561 tgtcgccggc ttgcggttta atgaacccat atcccttggt cgggttgaac cacttgacgg
     7621 tacccttcgg catcgcctgt ctcctggtct agacgtaaaa aagacgcggc gacgcttgac
     7681 gcgtgccacg ccacaaacgg gtttccggtg gtctgctcaa tttccgagtc ttaggtatca
     7741 agttcgcaaa acgacagttg atcggccaaa tccgattgca aggcggatag caacacgaaa
     7801 ttggtccctt agcaagcttc tggcccggct caggaaatcg tcccgggcgg catagctgtg
     7861 gcagcggagg cacaatttcc gccaattggc cgcaggggcg cggtcaagca attgaccccc
     7921 gacgggaacc cggggcaaat cggcttcaac cgacagtact ttttgcgttc gttcccccct
     7981 ttgaaaacgg catcatgtgc tgcccatcac ggccttcgaa ggtagagaac agaggggttg
     8041 cgccgtcctg cccgccctgg ttttcgtgct cgcggcgatc ggttcgtcgc cagtcttcgg
     8101 gcagggggcc ttcgtacctg ggccagcgcc gacgtccgtt ccaacgccga tagacatcaa
     8161 ttccgagatt gcgtcaggcg cccaggttct tgatctcggc ggcagtttcc tcgagcggct
     8221 tggcaaccag gccgccggcg gctttgggcg ggcgctgcgc aataatcccg gcggcggcgg
     8281 tgcctcggaa gcagtcgaag ggccgcgctt tcgaagctgg ggcgaggttt acggcctttc
     8341 gcttacgacc ggcccgcaag ccgattttct tggcgatagg cggcagaccc tgggtggcgt
     8401 ggtgggcttc ggtgccggca tcgcgccagg cgtcaatgtc ggcttctcgg ttgaccagag
     8461 ccacaccgcc atcgacgtgc cgctggcgat acagtcggcg acgatcgact tgacgcagct
     8521 cggtttcaac gcgtccgtgg acaagggacc gtggacctgg gcgatcgcgc tggtgtacgg
     8581 cttcggcaat gtcggctcaa gccgcgacac cgggcagggc ctcgccctcg caggctacgg
     8641 cactcaactc tacggcgccc tcaccgagct caactattac tgggatctgc agcaaagccg
     8701 cgtcgtgccg aaactcgatt tcgaatatgt gcgcgctacg acaggtgcat ttcaggagac
     8761 gggcgggctc aatccggtga gtgcgacggg cgctacgctg gaacgcgcgc gggtcttggt
     8821 gggcgccgag atcgggcact actggatctt cgaccggaac gtcctcgacc tgtcggccta
     8881 tggcaaattc gtcgacaatt tctcgcagag cttcagttct gtcgtcgtca gtctcggacc
     8941 gcaggccatc gcggtgcagg gtatcctcga aagccagtat ggcgccgata ccggcgcgtc
     9001 cgcttcgctc tcgctcggca acatgacgag gatctatctc aactatgacg gcaagttccg
     9061 ctccgcgctg caatcgcatc aggggacgct gggcttcgaa gccaggtggt agccgaagat
     9121 cgccgggcgg cgcaatcagc cggggcttgc cgcaacgtag ccggacaagc cgaagctctc
     9181 ccgtgcagtc gccatcattg gcaccaatgc attgggatga atgaactcgt ccctgaagca
     9241 ggcataggtc ttgggatcga aaatgctctt cagccaatcg atgacgatct tgtgccggtc
     9301 cgagtttcgg aattctcgat gataggtcag ccagaggtct gcatgatgac tgaagccatc
     9361 catgtcgacg gcgaccaacg gcgcgcccaa ggcgatagac gaggttggca ggaagcctat
     9421 gcccgcgcct ctctcgaccg catataggac cccgatactg gagtttgtct ttatcccgac
     9481 gacgccttcg agcgatttca gtcccaaaac ccttgcgtag gcggtgtcgt caacctgcgg
     9541 agcattttgc ttgatgattc tgtgattcct gagttcggac agggatgatg gcgttccgta
     9601 cagcttctgg taccctttgg aaacgaaggg atagatgtgc agccgtccga gcttggccac
     9661 gatcagttcg ggattcgtcg gcggttcaag ctgaatggcg atatcggatt ccagccgcgc
     9721 cacgtcggcc tgctccatcg cgcagcgaag gtccaccgtg attttcgtgt aggtcttctg
     9781 gaaatcgata agcctcggca gaatccagaa attgccgggg ccttcggtca cggccacgcg
     9841 cacggtccct gcggtgtcgt tggcggattt ggcggcgcgc cgaaacacat tgaaggcgtg
     9901 acgttccatc tccgtcacat cagcgagcat cgccgcgcct tcttcactca gcgtcagccc
     9961 gctctgatcg cgcaagaaca atctgcagcc gatccgctct tcgagccggt cgatcctgcg
    10021 catcagtgtg gtgctcgtga cctgcagttc ctcggccgcg ttccggaagc tcttgcatct
    10081 tgcacaggcc aggaaggact tcaaatcgtc ccacgatgca tcgagaacct cgtcagtccg
    10141 gtgctgcatc gtcgcagcac cccggtgcaa tagttgctgc atacccgaaa tccctaccca
    10201 gtatggtacc cgccgaaagg caggagtcac gataaatgcg cgatccgatt aaggaaagag
    10261 ggcctttggc ggaaagacat gatttcgatt cactgccgac cgacggggat atcacggtaa
    10321 aatgtgctcg gtacgtcgaa ctttcgcaat tggccgatat ggcgcaccgc ctggtgccgg
    10381 gagtacggat gacggcggat atcctcggca aataccttgc gtgggatccc gaatgcatcc
    10441 tgacattcag ccgccaggag agattgctgg gcgcgatagc ctttctttat ctcaacgatc
    10501 gtggacacga tgcgcttctc ctcgatgaaa tttgcttgac acagccggaa atcgaattcc
    10561 tcgccgcgcg cggcgaggag gtgtcggcca cttatatctg ggcctgcgca atcaccgggc
    10621 gtggcatctc cggttacggc aaggtggcgg agtatttgag aaggccgcgc tatgtcaacg
    10681 ccgatgtctt tgcccagccg tcgaccaagg cgggtcgcga ctggctcgtt gcgacatgct
    10741 tcaagcaaac acagagcttc cagcccgatc tctggtgcta cgaacggtcg tggaatcgcc
    10801 tgtcaatacc cgtgccagcg tcgggtgtat tagtaaggag ttatgcagat gcacggcact
    10861 agaccatccg cgctgcaagg cgccgaccaa cgcgcaatca cggtccgcat cgcgcgcgat
    10921 cccaacgatc tcatgctggt gacggcaatc cgctccgcgg tctatctcgc cgaacaggat
    10981 tgtccgctgg aagaggagtt cgacggcaac gatctggtgg ccgcgcactt cctgggcttt
    11041 gtcggcaagg agcctgctgg ctgtcttcgc gttcgctttt tcggcgagtt cgccaaggtc
    11101 gagcggctcg ctgtgcgcca tcagtaccgt cgcacgcggg t
//
LOCUS       DATLJT010001269        19464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2628417,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001269 DATLJT010000000
VERSION     DATLJT010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19464
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2628417"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VM910_29100"
     CDS             <1..1168
                     /locus_tag="VM910_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66603.1"
                     /translation="WKRFVPAEDDAFNAIVAAFSKATGTEMNVFSESFEDVQPKASVA
                     ANTGSGLDLAWGLHTLPQLFPTKVLLMNDVADHLGKKYGGWTEAAAKTCKHGEDWLGI
                     PIATIGGYMTYRKSATDKAGFKEFPKDFPGFLEMCKALKANNTPAGFALGHATGDANG
                     WLHWALWGHGAYTVSEKDQVIINSPETVKALEWVKAVSDNFIPGVASWNDSSNNKAFL
                     AGELYCTSNGISIYIAAKDDPTKKELAEDTYHALWPVGPIGKPTELQLAVPILAFNFT
                     KYPNAAKAFIDFMLEKEQFDAWLTGARGYLTHTLNAYDNSPIWTADPKNTVFGQASKR
                     ALPASGIGTPGEKAATAIADFIVVDMFANYCTGAKDAKGAIVDAERQLKRIYRG"
     gene            1227..2186
                     /locus_tag="VM910_29105"
     CDS             1227..2186
                     /locus_tag="VM910_29105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI66604.1"
                     /translation="MADIAVAPQRAKPEIREATTWDQLKHNRNWLGFWFMLPALAFLV
                     LFLAYPLGLGIWLSFTDTRIGRIGQFIGTENYEWLWDDAIFWLSVTNTILYTAIASAF
                     KFAIGLYLALLLNENMPFKAMLRAMVLIPFIVPTVLSALAFWWIFDSQFSIISWSLKH
                     LGLITQNINFLGDTTWARICVIFANIWRGVPFVAITLLAGLQTVQPSLYEAATLDGAT
                     RWQMFRHITYPLLTPIIAVVMTFSVLFTFTDFQLIWAMTRGGPVNATHLMATLSYQRA
                     IIAGQLGEGAAISSAMIPFLLAAIMVSWFGLQRRKWQQGENND"
     gene            2257..3102
                     /locus_tag="VM910_29110"
     CDS             2257..3102
                     /locus_tag="VM910_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVI66605.1"
                     /translation="MSYLQSVPRRLVTLYLPLSIIVVVLLFPFYWMALTSVKPDAQLL
                     DMETYSPFWTWNPTFKHFHKLLFESHYPHWLWNTMYVAVCATVLSIAASVLAAYAIVR
                     LRYKGANLVGGLIFLAYLVPPSILFIPLATVVFQYGLFDSPLALILTYPTILIPFSTW
                     LLMGYFKTIPFELEECALIDGASRWQILIKIVMPLAVPGLISAFIFCFTLCWNEFIYA
                     LTFLQSTQNKTVPVAIVNEFVDGDVYRWGALMAGALAGSLPLVILYAFFVEHYVSAMT
                     GAVKE"
     gene            3151..4137
                     /gene="denD"
                     /locus_tag="VM910_29115"
     CDS             3151..4137
                     /gene="denD"
                     /locus_tag="VM910_29115"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HVI66606.1"
                     /translation="MHVLVLGAAGMVGRKLVDRLLRGCRIGQSDITRLTLHDVVAPAK
                     PEKPGMPVDVHAGDFAVPGAAEKLIAGRPDVIFHLAAIVSGEAELDFDKGYRINLDGT
                     RMLLDAIRLAGNGYKPRVVFTSSIAVFGAPFPDAIGDEFFNTPLLSYGTQKAIGELLL
                     ADYSRRGFMDGIGIRLPTICIRPGLPNKAASGFFSNILREPLAGKDAVLPVSEDVRHW
                     HATPRSAVGFLLHAATLDLGTVGPRRNLTMPGLSATVGEQIAALKRVAGEKVAARIKR
                     VPDPFIMGIVEGWPRNFEAKRARQLGFTTAENSFDDIIRIHIEDELGGNFVR"
     gene            4327..5496
                     /locus_tag="VM910_29120"
     CDS             4327..5496
                     /locus_tag="VM910_29120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HVI66607.1"
                     /translation="MTASIVGWAHTPFGKFDAETVESLVVKVANEALADAGISASDVD
                     EIVLGHFNAGFSPQDFTASLVLQADPALRFKPATRVENACATGSAAVHQGIRAIACGA
                     AKTVLVVGVEQMTRTPGPEIGRNLLKASYLPEDGNIAGGFAGVFGKIAQSYFQKYGDQ
                     SDALAMIAAKNHKNGVANPYAQMRKDFGFEFCRAESEKNPYVAGPLKRSDCSLVSDGA
                     AALVLADAETAKAMGKAINIRATAHAQDFLPMSKRDILQFEGCTVAWQRALERAGLKL
                     SDLSFVETHDCFTVAELIEYEAMGLTPKGQGARAIKEGWTQKDGKLPINPSGGLKAKG
                     HPIGATGVSMHVMTAMQLAGQAPEGMQLRNPQLGGIFNMGGAAVANYVSILEPAK"
     gene            5605..5994
                     /locus_tag="VM910_29125"
     CDS             5605..5994
                     /locus_tag="VM910_29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVI66608.1"
                     /translation="MTLLRVRPWSAGAFALGLILVFASTVLQGLFFRFGVELYFATFF
                     PAVFLTALLAGRPAAALVVAVTVPLVWWAFIPPYFEFNTLVAAHIDAITMFLLLSLLL
                     IFLADGCRETIALLDASPRSGELSSEL"
     gene            complement(6100..6516)
                     /locus_tag="VM910_29130"
     CDS             complement(6100..6516)
                     /locus_tag="VM910_29130"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66609.1"
                     /translation="MSMQGRKLVHSDIKPENPPERAAHYLDVPNMPWEPTKFPGIRIK
                     VLYTDGTGITTALFKLEPGAVVPLHEHTALEQTYVIEGSLEDHEGKCGPGQFVWRPAG
                     NQHEAVAPNGAVLLGFFLKPNRFAYGEKFFTEPGER"
     gene            complement(6711..7079)
                     /locus_tag="VM910_29135"
     CDS             complement(6711..7079)
                     /locus_tag="VM910_29135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HVI66610.1"
                     /translation="MPLLRPLDPAFPIERQITIDASAVVLVNVFTLDRADEETFLRVW
                     QDDARFMKQQPGFISTQLHRAIGDSPAYLNYAVWETNADFRAAFTHPEFRAKISAYPS
                     SAVASPHLFQKVAVPGICVA"
     gene            7174..7647
                     /locus_tag="VM910_29140"
     CDS             7174..7647
                     /locus_tag="VM910_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI66611.1"
                     /translation="MNRTRRTPAGDALTDLMLDLFRLNSLLLTSGDRLVARLGLTSAR
                     WQILGAIVTADRPQPVAWLARNRGANRQNVQRIVNDLHREGLVGFGANPHHRRAQLVV
                     LTDKGKQTFDAAMRLQAPWANSLSEGLVVKDIQTVHRVTIALRKKLESRELDEMA"
     gene            7804..8643
                     /locus_tag="VM910_29145"
     CDS             7804..8643
                     /locus_tag="VM910_29145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI66612.1"
                     /translation="MKAILVPTQTIPAMKSTLETAVLLAQHTGAYIEGVPLWFGVPEF
                     VVAELASSFSIETYRGRREEETAEARKLFETFMHEHGLAASRTAPDRPSFGWFAEVPP
                     GEDLVGSHGRAFDVIVMGRPDAETTAPYHRAIESGLFESGRPVLLSPPVAPKQIATNI
                     MIHWNGSTEQARANAFAMPLLRLAKRVTVLTVVGGQDVPGPSADLVRRQLRFNGISAE
                     PVRVELEGRSTGEAVLAAAEADGCDLLVKGAFTRHRLRQLIFGGATSHILEHADLPVL
                     MAH"
     gene            9004..10212
                     /locus_tag="VM910_29150"
     CDS             9004..10212
                     /locus_tag="VM910_29150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI66613.1"
                     /translation="MGPLQGIKVIDMTTVLMGPYATQMLGDYGADVIKVESLEGDVTR
                     LIGPFRHPGMGPVFLNTNRNKRSICLDLKKPAGREAVLRLIKTADVLVYNVRPQAMAR
                     LNLGYDVVSEINPRLVYAGVFGFGQDGPYATKPAYDDLIQGATGLPALMAQTSDGIPR
                     YVPNALVDRIVGLTAVGAICASLVNRDRTGRGQRVDIPMFETMAGFVMGDHMGGLTYE
                     PPLDKGGYARHLSRDRRPYKTKDGYLSVIVYNDKQWESFFKATGRDDLRRDAKFATFA
                     ARAANIDIVYGELARIFETRTTDEWIELLTKADVPVMPMHDLETMLQDEHLVAIGYFP
                     VVEHPTEGPIRSMKVSAAWSDTPAESVRLAPRLNEHGAEILSEAGFSGEEIAALVRDG
                     VTKLSPATQG"
     gene            10217..11383
                     /locus_tag="VM910_29155"
     CDS             10217..11383
                     /locus_tag="VM910_29155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI66614.1"
                     /translation="MDFALTANQESIRDAVARICARFDDAYWLKKDKEGGFPADFHKA
                     LADAGWLGICIPEEFGGSGLGILEAAIMMRTISESGAGMSGASAIHMNVFGLNPVVVF
                     GTKEQCQRMLPGIVSGREKSCFAVTEPNTGLNTTQLKTRAVKKNDRYVVNGQKVWIST
                     AQVAEKILLLARTTPLEEVKNPTHGLSLFYTDFDRSRITVHEIEKMGRKPINSNELFF
                     ENFEIPFEDRLGDEGRGFEYILHGMNPERILIAAEAVGLGYLAMSRAAAYAKTRIVFN
                     RPIGQNQAIQHPLAKNWMELEAAWLMTLSAGWQYDGGFPCGPAANAAKYLAAEAGFHA
                     CEQAVMTHGGFGYAKEFHVERYLRESLIPRIAPVSRELIMSFIAEKVLGLPKSY"
     gene            11560..12696
                     /locus_tag="VM910_29160"
     CDS             11560..12696
                     /locus_tag="VM910_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI66615.1"
                     /translation="MSYITGVGLTSYGKHEGSSSFSLMSQAATLAISDAGLKRTDIDG
                     VLCGYSTVSPHIMLATVFAEHFGIRPSYAHAVQVGGATGLAMAMLAHHLVDAGVVRHV
                     LVVSGENRLTGQSRDASIAALAQVGHPDYEVPLGPTIPAYYGLVASRYMHEYGLTQED
                     LAEFAVLMRTHALDHPGAQFQEPITVADVMASKPVALPLKLLDCCPVSDGGAAFVVSR
                     QPTATIRVRVRGCAQAHTHQHVTAAPALSDLGAEISIARAKAAAGIAISDVRYVAIYD
                     SFTITLAMLLEDLGLAGRGEAAARIRAGYFGRDGEMPLNTHGGLLSYGHCGVGGAMAH
                     LVETHLQMTGRAGRRQVRDASLALLHGDGGVLSSHVSMFLERVR"
     gene            12693..13052
                     /locus_tag="VM910_29165"
     CDS             12693..13052
                     /locus_tag="VM910_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HVI66616.1"
                     /translation="MSEKPADWTNGHEAILYQHCGSCGRVQYFRRDFCAACGSVMLTE
                     SCASGEGMVYATSLVCRAATPEARAHVPYTIVLVDAKEGFRMMAHGDRDLAIGDPVTA
                     RFVPFAGRIVPYFEKVK"
     gene            13525..14049
                     /locus_tag="VM910_29170"
     CDS             13525..14049
                     /locus_tag="VM910_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI66617.1"
                     /translation="MARIDYSDPSKYNDRTRELLAKNRNANIFRMMAHSPSHLEQYCR
                     LGGAIRSKGELDPVVRELAITRTGILCEAPYEIVAHKRIGKNVGVTDEQNAALENWQA
                     AGCFNDVQRAALAFADEIVRLRRPTDATFAAIRSKLSSAALVELQLAVGFYIMTSKFL
                     ETFDIDMQPVTEVV"
     gene            14178..15308
                     /locus_tag="VM910_29175"
     CDS             14178..15308
                     /locus_tag="VM910_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HVI66618.1"
                     /translation="MSDTYRSIADEVEAAVAAGSPDKCFETLKSVAGLLSASAGSLSE
                     EQVALFGDVLDRLINTIELRSLAEIGARIALAELSKQLAPMGQAPTSVVRRLARNDDI
                     SVAEPVLIESARLSPEDLVEIARNKSEQHLLAISGRWWLKEVVTDALLARRYRSVSRR
                     LMRNPGARVSPAGFAIVVSQAETDPELAVETGIRVDLPAELRNQLLRSATEAVRTRLL
                     SRAPADLFEGIRKAISAASKSVDRQMSKMHDFESAMLLIGRLKVEDRLNEAVLGDFAR
                     QRRYEETVTALAELSQSNVEVVRSLMQSLRSDGILVPCKVADLGWQTVISVLDCRFAT
                     GATAPDELAKLKRQFADLSAADARRLLRLWTVRSPSPTSKLN"
     gene            complement(15313..17163)
                     /gene="glsA"
                     /locus_tag="VM910_29180"
     CDS             complement(15313..17163)
                     /gene="glsA"
                     /locus_tag="VM910_29180"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HVI66619.1"
                     /translation="MRQTPVSHAGNWSAAMPPLQRFLTACHADFTSDHGGEVAQYIPE
                     LSKANPEHFGISLATLDGHVYEVGDTGVPFTIQSISKPFVFALALDTLGADRVESEIG
                     VEPSGDPFNSIRLDAKNHPFNPMVNAGAITCSGLIRQVKGDGAFEYIRQALGRFAGRE
                     LDMDEAVFASESTTGDRNRAIGYLLRNSGVIKDDVRAVLEVYFQQCAVLVTARDIAIM
                     AATLANRGTNPVTGEQVVTPYAISRTLSVMTSSGMYDYAGEWIYRVGIPAKSGVGGGI
                     LAALPARLGMGSYSPRLDSHGNSVRGIKVCEALSSHYDLHMLNRSDDARTSVIADYHI
                     DKSSSRRSRRPQERKILAAHHNDVRVLELVGTLSFSNVDYVSRQLQGKPRPQFIIFDL
                     RRVTAMTPAGGRLLAEGFRELAASNVTAILSGIDRASAQWRRIREMTAGLSNIRNYPL
                     LDQAIEWAEDQIIYRHGGNIYANETTELSEQALLAGLAASELDLLASLGTINTYAAGE
                     KIVSAGEPASSLYFLKRGVVHVMLPGGGRLATITAGMAFGEMALLESHRSADVFADAS
                     VTALEVPLAAFERFRKEHPATSEQIMRNLARLLADRLISANTKMNLLTAT"
     gene            complement(17304..17525)
                     /locus_tag="VM910_29185"
     CDS             complement(17304..17525)
                     /locus_tag="VM910_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HVI66620.1"
                     /translation="MPDISVVTEVAGRVCALPLQTGASVGDGDDIAFVEAMKMEIPVA
                     SPAAGILKSLLVALDDMVAEGQVVAIIET"
     gene            17619..18590
                     /locus_tag="VM910_29190"
     CDS             17619..18590
                     /locus_tag="VM910_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI66621.1"
                     /translation="MGRRSERLSRHGMLAGDAGEGDVIQVVSRAFDVLRCFEGHEARL
                     GNLEISNRCGLPRSTVSRLTHTLTRMGQLVYLPRDQKYRIGPSAVAMSTSMMKGLQLR
                     NLIKLRLQDVAEHLPGTVGFVIPDRFHLVYLEYARAANALGLHDVTGSRIAMATTAAG
                     HAYTAALAPEIGDALLAEMEREIPDVAKVLQPRIENNRRFLREHGYVVACGLWSPHIN
                     GLAVPLWSPQYQTFVVVTIGLLAAMYDERRLHREVAPPMIELGRAVGELFEGAEGDLF
                     NNRLERKSLPVRTNNNKIIKTEETNDLEARTRRPRSARSLRARDGRR"
     gene            18520..>19464
                     /locus_tag="VM910_29195"
     CDS             18520..>19464
                     /locus_tag="VM910_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVI66622.1"
                     /translation="MIWKPELDDLARREAFAREMGGVEKVKRQRDQGRLTVRERIDKL
                     VDANSFHEVGAISGIGEYDEHGELKHLTPANCVFGRARVNGRTVVVVGDDFTVRGGSA
                     DASISAKPLMAEEMAHDFRLPIIRIIEGSGGGGSVKTIETKGAANLPGGIGGTRWYRY
                     TTENLAHVPVVALGLGSVAGLGAARLAASHYSIMTRDSAMFVAGPPVVKRLGQDLSKQ
                     KLGGADIQTRAGGVDDAVDTEEEAFEKAKRFLSYLPSSVFELPPTLPCDDDPERSEEI
                     LMKAVPRSRRQVYKMRPIIEAVVDKGSFFEMNANFGRPM"
BASE COUNT         3652 a         5917 c         6103 g         3792 t
ORIGIN      
        1 ctggaagcgc ttcgtgccgg cggaggacga cgcgttcaac gccatcgtcg cggcgttttc
       61 gaaggccacc ggcaccgaga tgaacgtgtt cagcgagtcc ttcgaggatg tgcagccaaa
      121 ggcgtcggtc gccgccaata cgggctcggg tctggatctc gcctggggct tgcacacgct
      181 gccgcaactg ttcccgacca aggtcctcct gatgaacgac gtcgccgatc atctcggcaa
      241 gaaatacggc ggatggaccg aggcggctgc aaagacctgc aagcacggcg aggattggct
      301 cggcatcccg atcgccacca tcggcggtta catgacctac cgcaagtcgg cgaccgacaa
      361 ggcggggttc aaggagttcc cgaaggactt tccgggcttc ctcgaaatgt gtaaggcgct
      421 gaaggcgaac aatacgccgg cgggctttgc gctcgggcat gccaccggtg acgccaacgg
      481 ctggctgcac tgggcgctct ggggtcacgg cgcctacacg gtcagtgaga aggaccaggt
      541 gatcatcaat tcgcctgaaa cggtcaaagc gctcgagtgg gtcaaagccg tgtccgacaa
      601 cttcattccg ggcgtggcgt cgtggaacga ttcctcgaac aacaaggcgt tcctcgcggg
      661 cgagctctac tgcacctcga acggcatctc gatctatatc gccgccaagg acgatccgac
      721 caagaaggaa cttgcggagg acacctacca cgcgctgtgg ccggtcggtc cgatcggcaa
      781 gccgacggag ctgcagcttg cggttccgat cctcgccttc aacttcacca aatatccgaa
      841 cgccgcaaaa gccttcatcg acttcatgct ggagaaggaa cagttcgacg cttggctgac
      901 gggtgcgcgc ggctatctca cccacacgct caacgcctat gacaattcgc ctatctggac
      961 ggcggaccca aagaacacgg tgttcggcca ggcctcgaag cgcgcgctgc cggcgtccgg
     1021 catcggcacg ccgggcgaga aggcggcaac cgcgatcgcc gacttcatcg tggtcgacat
     1081 gttcgctaat tattgcaccg gcgcgaaaga cgccaagggc gccatcgtgg acgccgaacg
     1141 gcagttgaag cgcatttatc gcggttgaac gtacgacgcg ggcggtgatg ttgccgcccg
     1201 cgtctttatt ttttgggatg acgcccatgg ccgatattgc cgtcgcgccc cagcgcgcca
     1261 agccggaaat ccgcgaagcg accacgtggg accagctcaa gcacaacagg aactggctgg
     1321 gcttctggtt catgttgccg gcgctggcgt tcctcgtcct gtttctcgcc tatccgctcg
     1381 ggctcggcat ctggctgtcc tttaccgaca ccaggatcgg tcgcatcggc cagttcatcg
     1441 ggaccgagaa ctacgagtgg ctgtgggacg acgcgatctt ctggctctcg gtcaccaaca
     1501 cgatcctcta taccgcgatc gccagcgcct tcaaattcgc aatcggcctt tatctcgcgc
     1561 tgttgctcaa cgagaacatg ccgttcaagg cgatgctgcg cgcgatggtc ctcattccct
     1621 tcatcgttcc gaccgtgttg tcggcgctgg cgttctggtg gatcttcgat tcgcaatttt
     1681 cgatcatctc gtggtcgctg aagcatctcg gactgatcac ccagaacatc aattttctgg
     1741 gcgacaccac ttgggcgcgg atctgcgtga tcttcgccaa tatctggcgc ggcgtgcctt
     1801 ttgtcgcgat cacactgctg gcgggactgc agaccgtgca gccgtcgctc tatgaggcgg
     1861 caacgctcga cggcgcgacg cgctggcaga tgttccgcca catcacctat cccttgctca
     1921 ccccgatcat cgcggtcgtg atgacgttct cggtcctctt caccttcacc gacttccagc
     1981 tcatctgggc gatgacccgc ggcggtccgg tcaacgccac gcatctgatg gcgacgctct
     2041 cctatcagcg cgcaatcatc gcgggacaat tgggcgaggg cgctgcgatc tcgagcgcca
     2101 tgatcccgtt cctgctcgcc gcgatcatgg tgtcgtggtt cggattgcag cgcaggaagt
     2161 ggcagcaggg agaaaacaat gactgacctc ccagcatcga aagtcgacct caagaccatc
     2221 ctcgcgaccc cgtcgcgggt cgataacagc gaaggcatga gctacctgca gtcggtgccg
     2281 cgcaggctgg tgacgcttta cctgccgcta tcgatcatcg tcgtcgtgct gttgtttccg
     2341 ttctactgga tggcgctgac ctcggtgaag ccggacgctc agctcctgga catggagacc
     2401 tacagtccgt tctggacctg gaatcccact ttcaagcact tccacaagct cttgtttgaa
     2461 agccattatc cgcactggct gtggaacacg atgtatgtcg cggtgtgcgc caccgtactc
     2521 tcgattgcgg ccagcgtgct cgcggcctat gccatcgtgc gcctgcgtta caagggcgca
     2581 aacctcgtcg gcggtctgat cttcctggct tacctcgtac cgccctcgat cctcttcatt
     2641 ccgctggcga ccgtcgtttt tcagtacggc ctgttcgatt cgccgctcgc actgatcctg
     2701 acctatccga ccatcctgat cccgttctcg acctggctgt tgatgggtta tttcaagacc
     2761 attcccttcg aattggagga gtgcgccctg atcgacggcg caagccgctg gcagatcctg
     2821 atcaagatcg tgatgccgct tgctgtcccc ggcctgatct cggccttcat cttctgcttc
     2881 acgctgtgct ggaacgagtt catctacgca ctcacctttc tgcaatcgac ccagaacaag
     2941 acggtgccgg tcgcgatcgt caacgagttc gtcgacggcg acgtctatcg ctggggcgcg
     3001 ttgatggcag gcgcgcttgc gggctcgctg ccgctcgtca tcctttacgc cttcttcgtc
     3061 gagcattatg tgtcggccat gaccggagcc gtgaaggagt gagcgcgccc tttgcgcttc
     3121 aagaattaag aacggacgag gagttggctc ttgcacgtat tggttctggg cgcggccggc
     3181 atggtcggcc gcaaactggt tgatcgtttg ttgcgcggct gccgtatcgg ccagtcggat
     3241 atcaccaggc tgaccctgca cgacgtcgtc gcccctgcaa agccggagaa gccgggcatg
     3301 ccggtcgacg tccacgccgg cgatttcgcc gtgcccggcg ccgccgaaaa gctgatcgcc
     3361 ggccgccccg acgtcatctt ccatctggcc gcgatcgtct cgggcgaagc ggagctcgat
     3421 ttcgacaaag gctatcgcat caatctcgac ggtacgcgga tgctgctcga tgccatccgc
     3481 cttgcaggaa acggctacaa gccgcgtgtg gtcttcacgt cgtccatcgc ggtgttcggc
     3541 gcaccctttc cggacgcaat cggtgatgag ttctttaata ccccgctcct gagctacgga
     3601 acgcagaagg cgatcggcga actcttgctc gcggattatt cgcgccgcgg tttcatggat
     3661 gggatcggta tccgtctgcc gaccatctgt atccggcccg gccttccgaa caaggcggcg
     3721 tcgggcttct tttccaacat cctgcgcgag ccgcttgccg gcaaggacgc ggtgctgccg
     3781 gtatcggagg acgttcgcca ttggcatgcc acaccgcgtt cagcggtcgg tttcctgctg
     3841 catgcggcga ccctcgatct tggaacggta ggtccgcgcc gcaacctcac catgccgggt
     3901 ctctccgcga ctgtgggcga gcagatcgcg gctttgaagc gcgtcgccgg agaaaaggtg
     3961 gcggcccgca tcaagcgcgt gccggatccc ttcattatgg gtatcgtcga gggctggccg
     4021 cggaatttcg aggccaaacg agcacgccag ctcggattta ccaccgccga aaacagcttt
     4081 gacgatatta tccggatcca catcgaggac gaacttggcg gtaacttcgt caggtgagcc
     4141 gcgagatgag gaggttggca ttcgttgcgg tcaatcggtg gcttcacaaa acgaatgtca
     4201 aatccaggcc ccactggcct ataaaggatg ctggcggtgc cgatataacc ttcgccgaga
     4261 tcgcccgctt cgatgggcgc gaggctaaca atcagaccgc gagcgcggat ttttgggaga
     4321 gcaatcatga cagccagcat tgtcgggtgg gcgcacacgc ccttcgggaa attcgacgcc
     4381 gagaccgtcg agagtcttgt cgtcaaggtc gcgaacgaag cgctggcgga cgccggcatc
     4441 tccgcgagcg atgtcgacga gattgtgctc gggcatttca atgccggctt ttcgccgcag
     4501 gatttcacgg cttcgctcgt cctgcaggcc gatccggcgc ttcgcttcaa gccggcgacg
     4561 cgggtggaaa acgcctgcgc cacgggatct gccgccgtgc atcagggtat ccgggcgatt
     4621 gcctgcggcg ccgccaagac cgtcctggtc gtcggcgtcg agcagatgac gcgcacgccg
     4681 ggccccgaga ttggccgcaa tctcttgaag gcctcgtatc tgcctgagga tggcaacatc
     4741 gccggcggct ttgccggcgt gttcgggaaa atcgcgcaga gctatttcca gaaatatggc
     4801 gaccagtccg atgcgctggc gatgattgcg gccaagaacc acaagaacgg cgtcgccaac
     4861 ccctacgcgc agatgcgcaa ggactttggc ttcgagttct gtcgcgccga gagcgagaag
     4921 aatccctacg tcgcaggtcc cttgaagcgc agcgattgct cgcttgtgtc ggacggtgcg
     4981 gctgcgctgg tgctcgccga tgccgaaacc gcgaaagcca tgggcaaggc gatcaacatc
     5041 cgcgcgaccg cccatgcgca ggacttcctg ccgatgtcca agcgcgacat cctgcagttc
     5101 gagggctgca cggtcgcctg gcagcgcgcg ctcgagagag ccggcctcaa gctttccgat
     5161 ctctccttcg tcgagaccca cgactgcttc accgtcgccg aactgatcga atatgaagcg
     5221 atggggctga cgccgaaagg gcagggcgcg cgcgctatca aggagggttg gacgcaaaag
     5281 gacggcaagt tgccgatcaa tccttccggc ggtctcaagg ccaaaggcca ccctatcggc
     5341 gcaaccggcg tctccatgca tgtgatgacg gcgatgcagc ttgcaggcca ggccccggag
     5401 ggcatgcagc tcagaaatcc gcagctcggc ggcatcttca acatgggtgg tgctgcagtc
     5461 gcgaactacg tctcgatcct cgagccggcc aaataaatgt ccaagtaact gcggcaatgt
     5521 gagccgggcc ggttcttcac atggaaccat tctcgaccta aaatgcgagg gtggttcctt
     5581 gatttgcgag tctggtcaag cttgatgaca ttgctgcgag ttcggccgtg gtcggctggt
     5641 gcatttgcgc tcggccttat tctcgtgttt gcctcgacgg tccttcaagg cctgttcttc
     5701 cggttcgggg tcgagctgta ctttgcgaca ttcttccccg ccgttttcct gaccgccttg
     5761 ctggcgggaa gaccggcggc cgcactcgtc gtcgctgtca cggtcccgct ggtgtggtgg
     5821 gcgtttattc cgccctattt tgaattcaat acgctggtcg cggcccatat cgatgcgatc
     5881 acgatgttcc tgcttctcag cctgcttttg atctttctcg ccgacggatg ccgggagacg
     5941 atcgcgctcc tggatgccag cccgcgatct ggagagctgt ccagcgagct atagggcagg
     6001 caatggccgg cgccacgcac actggtcgtc cacgctacgc catgccgact gggcgccgag
     6061 ttcgatgcct gtttgagccc gacgctgacg agcgaggccc taccgctccc ccggctcagt
     6121 gaagaacttt tcgccatagg caaagcgatt gggcttgaga aagaagccga gcagcaccgc
     6181 gccgttgggc gcaaccgcct cgtgctgatt gccggcaggg cgccacacga actgaccggg
     6241 cccgcatttg ccttcatggt cctctagcga tccctcgatc acataggtct gctcaagtgc
     6301 ggtgtgctca tgcagcggca cgacggcgcc cggctcgagc ttgaacaagg ccgtggtgat
     6361 gccggtgccg tccgtataga ggaccttgat cctgataccc ggaaactttg ttggttccca
     6421 aggcatgttg ggcacatcca gataatgcgc cgcgcgttcc ggcggatttt ccggtttgat
     6481 gtcggaatgc acaagcttgc gtccttgcat ggacatgacg gactccctgg caatcggttt
     6541 tgcgtttttg ggaaacgggg acagcttgat gttgaggcac cgtcgtttgc gcacttcgga
     6601 agaagcacac tggcaacctg ttggtgaacc gccacactag cataccgcgt gcacggcatt
     6661 accgaaagtc gcaaacagac aaggctgcgg cgtcggcctt cgtggttggg ctaggcgacg
     6721 cagatgccgg ggaccgcgac cttctgaaac agatgcgggc tggctacggc cgaggacgga
     6781 taggccgaga tcttcgcccg aaattccgga tgcgtgaacg cagcgcggaa gtcggcgttc
     6841 gtttcccaga ccgcatagtt caaataggcc gggctgtcgc cgatcgcacg gtgtagctgg
     6901 gtcgaaatga agcccggctg ctgcttcatg aaccgcgcat cgtcctgcca gaccctgaga
     6961 aaggtctctt cgtcggctct gtcgagcgtg aatacgttca ccaacacgac ggcgctcgcg
     7021 tcgatcgtga tctggcgttc aatcgggaag gcgggatcca gcgggcgtaa cagcggcatg
     7081 gggcgctcca ttggtaatat ggtgtcaaca tggtattatg aaacatacat aactaattga
     7141 cattatgatg tcaatatagg ttaatggtga catatgaaca ggaccagacg gacgcccgcc
     7201 ggcgatgctc tgaccgatct catgctcgac ctgttcaggc tcaacagcct gcttttgacc
     7261 tctggcgatc gcctggtcgc acggctcggg ctgacgagcg cgcgctggca aattctcggc
     7321 gccatcgtga ctgccgaccg cccgcaacca gtcgcgtggc tcgcccgcaa tcggggtgcc
     7381 aaccgccaga acgtgcaacg gatcgtcaac gacctccaca gggaagggct cgtcgggttc
     7441 ggggccaacc cgcatcaccg gcgggcccag cttgtcgtcc tgactgataa gggaaagcag
     7501 acattcgatg ctgcgatgcg gctacaggcc ccgtgggcca acagcctttc ggaagggctc
     7561 gtggtgaagg acatccagac cgtccaccgt gtcactattg cgttgcgcaa aaaactggaa
     7621 agcagagagc tggacgagat ggcttgacgg tatccggcga gcactgacag cacgggatgg
     7681 taaggggctt tgggccaaat atcttggcgg tcctgtctcg aaggtctaag cttgtcaaag
     7741 tcagtggctg tttttggctg catcgggcaa tcgttcattt gcggcgagcg gggagagccg
     7801 acgatgaagg cgattttggt accaacccaa actattccgg ccatgaaatc cacgctcgag
     7861 acggcggtgc tgctcgcgca gcacacgggc gcctatatcg agggtgttcc gctgtggttt
     7921 ggcgttcccg aatttgtcgt agctgagctc gccagcagct tttccatcga aacttaccga
     7981 ggacgtcgcg aagaagaaac ggcggaggcg cgcaagctgt tcgagacctt catgcacgag
     8041 cacggtcttg cggcttcgag aacggccccc gaccgccctt cgttcgggtg gttcgccgag
     8101 gtgccaccgg gcgaagatct tgtcggcagc catggccgcg cgttcgacgt gatcgtcatg
     8161 ggcaggccgg acgctgaaac gaccgcgccc tatcaccgag cgatcgagtc ggggctgttc
     8221 gaaagcggcc ggcccgttct gctgtcgcca ccggtcgctc ccaaacagat cgcgaccaac
     8281 atcatgatcc actggaatgg cagcaccgag caggcccggg ccaacgcttt tgcgatgccg
     8341 ttactgcgcc tcgccaaacg ggtgacggtg ttgactgtcg tcggtggaca ggacgtgccg
     8401 ggtccgtcag ctgacctggt gcgcaggcaa ttgcggttca atggcatctc cgccgagccc
     8461 gtccgcgtcg agttggaggg acgcagcacc ggcgaggcgg tccttgccgc ggccgaggct
     8521 gacggctgcg atctcttggt caagggagcc ttcacaagac acaggctacg gcaattgatt
     8581 ttcggcggtg cgaccagcca tatcctggag cacgccgatt tgccggtgtt gatggcgcat
     8641 taggcgcgca acctgatttg cctaaaaacg ggcagatcag ttgcaagttc cggatggcgc
     8701 cttgcgcagg gaaggccggg agttccggtt gaaccggtgg tgaccacgct ggtgtgcttt
     8761 aaacttctgc acgccaggct gcgggtgcag ccagcgcccg gttttccccg agccctctga
     8821 tttttgaggg acgaaatcga tccaaaactc gagcgcgaag cgccgcggga acgagggcgc
     8881 gcgttccgga tacgacgcgg cgtgctgtgc caaaccgcat acccccatac gccggccgcc
     8941 cttgatctgg ccaacaccgt gccgttactt ggcagtacac aacatcaggg agcgaacgtc
     9001 atcatgggcc cgttgcaagg catcaaggtc atcgacatga cgaccgtgct gatggggccc
     9061 tatgcgaccc aaatgctcgg cgattacggt gccgacgtca tcaaggtgga gtcgctcgag
     9121 ggcgacgtta cccgtctgat cggccctttc aggcatcccg gtatgggacc ggtgtttctg
     9181 aacaccaatc gcaacaagcg ctcgatctgc ctcgacctga agaagccggc ggggcgggaa
     9241 gccgtgctgc ggctgatcaa aaccgcggac gtactggttt acaacgtgcg gcctcaggcg
     9301 atggcgcggc tcaatctcgg ctatgacgtc gtatcggaga tcaacccgcg cttggtctat
     9361 gccggcgtgt tcggcttcgg ccaggatggc ccctatgcta cgaagcccgc ctacgacgac
     9421 ctgatccagg gcgctaccgg cctgccggcg ctgatggcgc agacctctga tggcattccc
     9481 cgttacgttc cgaacgcgct ggtcgatcgc atcgtggggc ttaccgcggt cggtgcaatc
     9541 tgcgcaagtc tcgtgaatcg cgaccgcaca ggccgcggcc aacgcgtcga cattccgatg
     9601 ttcgaaacca tggcgggatt tgtcatgggc gatcatatgg gcggcctcac ttacgagccg
     9661 cccctcgaca agggtggtta cgcgcgccac ctgtcgcgcg accgcaggcc ctacaagacc
     9721 aaagacggct atctcagcgt catcgtctac aacgacaagc agtgggaaag ttttttcaag
     9781 gcgaccggcc gtgacgacct gcgccgggat gcgaaattcg caacctttgc cgcacgcgcc
     9841 gccaatatcg acatcgtcta tggcgagctt gcccgcattt tcgagacgcg cacgacggac
     9901 gaatggatcg agctcctgac caaggccgat gtccccgtga tgcccatgca tgatctcgaa
     9961 accatgttgc aggatgagca tctggtcgcg atcggctatt ttcccgtggt cgagcatccg
    10021 accgaagggc cgatccgcag catgaaagtg tcggcggcgt ggtcggacac gccggccgag
    10081 tcggtacgac tcgcaccgcg gctcaacgag cacggcgcgg agatcctgag cgaagccgga
    10141 ttctctggtg aggagatcgc ggcgctcgtg cgcgacggcg tcaccaaact gtcgcccgcc
    10201 acacagggat agagccatgg atttcgccct gactgcaaac caggaatcga tccgagatgc
    10261 cgtcgcaagg atctgcgcgc gcttcgacga cgcttactgg ttgaagaagg acaaggaagg
    10321 cggcttccct gccgactttc acaaggcgct ggcggatgcc ggctggctcg gtatctgcat
    10381 ccccgaggag tttggcggct cgggcctcgg catcctcgaa gccgcgatca tgatgcggac
    10441 gatctcggaa tccggcgccg ggatgtccgg cgcatcggcg atccacatga acgtgttcgg
    10501 gctcaacccg gtcgtcgtat tcggcacgaa ggagcaatgc cagcgcatgc tgcccggcat
    10561 cgtctcgggc agggaaaaat cctgctttgc cgtcaccgag cccaacaccg gcctcaacac
    10621 cacgcagcta aagacgcgcg cagtgaaaaa gaacgacaga tatgtcgtca acggccagaa
    10681 ggtctggatt tccactgccc aggttgcgga aaagatcctg cttcttgcgc gcaccacgcc
    10741 gctggaagaa gtcaagaacc cgacccatgg cttgagcctg ttttataccg atttcgatcg
    10801 cagccgcatc accgtgcatg agatcgagaa aatggggcgc aagcccatca attccaacga
    10861 gctgtttttc gagaactttg agattccatt cgaggatcgg ctcggtgacg agggccgcgg
    10921 cttcgaatac atccttcacg gcatgaatcc cgagcgcatc ctgatcgcgg ccgaggcggt
    10981 cgggctcgga tatctcgcca tgtcccgcgc cgccgcttat gcaaaaaccc gcatcgtctt
    11041 caaccggccg atcggccaga accaggcgat ccagcatccg ctcgcgaaga actggatgga
    11101 actggaagct gcctggctga tgacgctgtc cgccggctgg cagtatgacg ggggctttcc
    11161 gtgtgggcct gccgccaatg ccgcgaagta tctggcggcg gaagcaggtt tccatgcctg
    11221 cgagcaggcg gtgatgaccc atggcggctt cggctatgcg aaggagtttc acgttgagcg
    11281 ctatttgcgg gagtcgctga ttccccgcat tgctccggtc agccgggagc tgatcatgag
    11341 ctttatcgcc gagaaggtcc tgggcctgcc gaagtcgtat tgaaatatcg aagtcacgtt
    11401 gatgatgcgg ttggagatag tcagagacgc acgctgcttc gtctccctcc ctctgaaagg
    11461 gggagggctg gggagggggc cattgcacaa ctgccagttt gatcccctcc cggctcactg
    11521 cgtgagccga cctccctttt tcatggggga ggtaagaaaa tgagctacat caccggcgtc
    11581 gggctgacat cctacggcaa gcatgagggc tcttcgtcgt tcagcctgat gagccaggcg
    11641 gccacgctcg cgatttcaga cgccggactg aagcgcaccg acatcgatgg cgtcctgtgc
    11701 ggctacagca cggtttcgcc gcacatcatg ctcgccaccg tcttcgccga gcatttcggc
    11761 attcgtcctt cctacgcgca tgcggtgcag gtcggcggcg cgacggggct cgcgatggcc
    11821 atgctcgcgc atcatctggt cgatgcgggt gtggtcaggc atgtgctcgt cgtcagcggc
    11881 gagaacaggt tgaccgggca gagccgcgat gcctcgatcg ctgcgctcgc gcaggtcggc
    11941 catcccgact acgaagttcc gctcgggcct accatccccg cctattacgg tctcgtcgcc
    12001 tcgcgctaca tgcatgaata tggcctcacg caggaggacc ttgcggaatt cgcggtgctg
    12061 atgcgcacgc acgcgctcga ccaccccggt gcacaattcc aggaaccgat cacggtcgcc
    12121 gacgtgatgg cctcaaaacc ggtggcgctg ccgctaaaac tcctggactg ctgtcccgtc
    12181 tcggacggtg gcgcggcctt tgtggtcagc cgtcagccaa ccgcgaccat tcgcgtgcgt
    12241 gtccgcggtt gtgcgcaggc gcatacccat cagcatgtca cggccgcacc tgcattgagc
    12301 gatctcggcg ccgaaatttc gatcgcaaga gcaaaagcgg cagccggcat cgccatttcc
    12361 gatgtacgct atgttgcaat ctacgacagc tttacgatca cgcttgcgat gctgctggaa
    12421 gacctgggtc tcgctggacg cggagaggct gctgctcgca ttcgtgccgg ttatttcggc
    12481 cgcgacggag agatgccgct caacacccac ggcgggctcc tgagctacgg ccactgcggc
    12541 gtcggcggcg cgatggcgca cctcgtcgag acgcatctgc agatgacggg acgcgcggga
    12601 cgacgccagg tccgcgacgc gtcgcttgcg ttgttgcatg gcgatggcgg cgtcttgtct
    12661 tcgcacgtca gcatgttcct ggagcgggtg cgatgagcga gaagccggcc gattggacca
    12721 acggccacga ggccatcctc taccagcact gcggatcgtg tggccgcgtg cagtatttcc
    12781 gtcgcgactt ttgcgcggcg tgtggttcag tcatgctgac cgagagttgt gcaagcggcg
    12841 agggcatggt ctacgcgacc tcgctggtct gtcgcgctgc gacgcctgag gcgcgtgcgc
    12901 acgtgcccta cacaatcgtt ctggtcgacg cgaaagaggg ttttcgcatg atggcccacg
    12961 gcgaccgcga tcttgcgatc ggggatcccg tcaccgcgcg ctttgttccg tttgccgggc
    13021 gcatcgtgcc gtatttcgag aaggtgaaat gagggaagtc gccgttgcga ggagcgaacc
    13081 cggagcctgc tgcgaggcct taaatggcct tggggagccc gtcttcgggc cgcgcttcgc
    13141 gcgcacccgt cggctcgcaa tgacgacctt agcgccagta gaaaaggacg ctggcgatga
    13201 cgagcattgc cgtcaccgcc agcatgtgcg cgaatgcttc cgagctcgcc ctcttggtgg
    13261 cttccttcat gccggttctc tccctagacc tgggcgcgac tcatcgtggc gcgagctacg
    13321 cgcaacgcgg tcagaatttt tcactcggaa caccccgcga cgagtatccg ccttgcttga
    13381 agtccccatg cggcgaatat cgactgtgcc agcgtcgatc agtaatgcgg cggcaatttg
    13441 actcaagaat gtcggcgggc ggatagtgtt taggcgaaat catcgccgcg atcatcgtaa
    13501 gtcagcgcga acatgaggag aaacatggcc cgcatcgatt atagcgaccc cagcaagtac
    13561 aacgatcgca cgcgcgagct gttggcgaaa aaccgcaacg ccaacatctt tcgcatgatg
    13621 gcgcactcgc cgagccatct cgagcaatat tgccggctcg gcggcgcgat ccgcagcaag
    13681 ggcgagctcg atcccgtggt ccgggagctt gcgatcacac gtaccggcat cttgtgcgag
    13741 gcgccctatg agatcgtggc gcacaagcgt atcggcaaga atgtcggcgt caccgacgag
    13801 cagaacgctg ctctcgagaa ctggcaggcg gcaggctgct tcaatgacgt gcagcgtgcg
    13861 gcgctggcgt ttgcggacga gatcgtgcgg cttcgccgcc cgacggatgc gaccttcgct
    13921 gcgatccggt cgaaactctc gtccgcggcc ctcgtcgagc tgcagcttgc ggtcggcttc
    13981 tacatcatga cctcgaaatt cctggagact ttcgacatcg acatgcagcc ggtcacggag
    14041 gtggtctagg acccccgccg tcaattgccg gagcgggggt cgccgaaagc gaaaggttaa
    14101 acttttacga aacctttaga tggtgtggtt taggttcgcc tggccagaac agcaggcaac
    14161 accgctcagg atgagcaatg tcggatacgt atcggtcgat tgccgacgag gtcgaggcgg
    14221 cggtagccgc aggttccccg gacaaatgct tcgagacgct gaagagcgtc gcgggcctgt
    14281 tgtcggcgag cgccgggagc ctcagcgagg aacaggtcgc gctgttcggc gacgttctcg
    14341 accgtctgat caacacgatc gaacttcgct cgcttgccga gatcggcgcc aggatcgcgc
    14401 tcgccgaact gagcaagcag cttgctccca tgggccaggc gccgacgtcg gtggttcgcc
    14461 gcctggcgcg gaacgacgat atttcggttg ctgagcctgt cctgatcgag tcggcgcgcc
    14521 tcagccccga ggatctggtc gagatcgccc ggaacaagag cgaacaacat cttttggcca
    14581 tatccggccg ctggtggctc aaggaagtcg tcaccgatgc gcttttggcc cgccgttaca
    14641 ggagcgtcag ccgtcgtctg atgaggaatc cgggtgcaag ggtgtcgccg gccgggtttg
    14701 cgatcgtggt gtcgcaagcc gagaccgacc cggagctcgc ggtagagacg ggaatccgcg
    14761 tcgatctgcc cgcagagctt cgcaaccaac tgctgcgcag cgccacggaa gcggtccgta
    14821 cgcggctttt gtcgcgcgcg ccggccgatc tgttcgaggg catccgcaag gcgattagcg
    14881 ccgcttcgaa gagcgtcgac cgtcagatgt cgaagatgca cgatttcgag agtgcaatgc
    14941 tcctgatcgg caggctcaag gtcgaggacc ggttgaacga ggcggtgctt ggcgattttg
    15001 cccggcaacg cagatatgag gagacggtca ccgcacttgc cgagctatcg caatccaatg
    15061 tcgaggttgt ccgctcactg atgcagagcc tgcgcagtga cggcattctc gttccgtgca
    15121 aggtggcaga cctcggctgg cagaccgtga tctccgttct cgattgccgg tttgcgacgg
    15181 gagcaacggc tcccgacgag cttgccaagc tgaagcggca atttgccgat ttgagcgccg
    15241 ccgacgcgcg ccggctgctc cggctttgga ctgtccgctc gccttcgcca acatcgaaac
    15301 tgaattgagc tctcaagtcg cagtgagcag attcatcttg gtattggccg atatcagccg
    15361 gtcggcgaga agccgggcga ggttgcgcat gatctgctca gaggtcgccg gatgctcttt
    15421 tcggaaacgc tcgaaggcgg caagcggcac ttcgagcgcg gtgaccgagg cgtcggcaaa
    15481 gacgtccgcg gagcgatggc tctccagcag tgccatttcc ccgaacgcca tgcccgccgt
    15541 gatcgtcgca agccggccgc cgcccggcag catgacgtgc acgacgcccc gtttgagaaa
    15601 gtacagggac gaggccggct caccggcgct gacgatcttt tcgcccgccg cataggtatt
    15661 gatcgtgccg agcgaagcca acaggtcgag ctcgctggcg gcaagccccg cgagcagggc
    15721 ctgctcggaa agctcggtgg tctcgttggc gtaaatgtta ccgccgtggc gatagatgat
    15781 ctggtcctcg gcccattcga tggcctggtc gagcaaggga tagttgcgga tgttggacag
    15841 gccggccgtc atctcgcgga tccgtcgcca ttgcgccgag gcgcggtcga tgccggaaag
    15901 gattgcggtc acgttagagg cggcgagctc ccgaaagccc tccgcgagca gccggccgcc
    15961 cgccggcgtc atcgcggtga cgcggcgcag gtcgaaaatg atgaattgcg ggcgcggctt
    16021 gccttggagc tgccgcgaga cgtagtcgac gttggaaaac gacagcgtgc cgaccaattc
    16081 cagcacgcgg acgtcgttgt gatgggcggc gaggattttg cgctcctgcg gacgccggct
    16141 gcgtcgcgac gagctcttgt cgatgtggta gtcggcgatc accgaggtgc gcgcgtcgtc
    16201 actgcgattg agcatgtgca aatcgtaatg cgaagacagt gcttcgcaca ccttgatgcc
    16261 acgcacgctg ttgccgtggc tgtcgagccg cggcgaatag ctgcccatgc cgagccgcgc
    16321 cggaagcgct gccagaatgc caccgccgac gccgctcttg gcgggaatgc cgacgcgata
    16381 gatccactcg ccggcgtaat cgtacatgcc ggaggaggtc atgaccgaca gcgtgcgcga
    16441 gatcgcgtaa ggcgtcacca cctgctcgcc cgtcaccgga ttggtgccac gattggcaag
    16501 cgtcgccgcc atgatcgcga tgtcgcgtgc ggtgacaagc accgcgcatt gctgaaaata
    16561 gacctccagc accgcgcgca cgtcgtcctt gatcacgccg gaattgcgca ggagataacc
    16621 gatcgcgcgg ttgcggtcgc cggttgtgct ctccgacgcg aagaccgctt cgtccatgtc
    16681 gagttcgcgg cctgcaaagc ggccgagcgc ctggcgaata tattcgaagg cgccgtcgcc
    16741 cttgacctgg cggatcaagc cggaacaggt gatcgcgccg gcattgacca tcggattgaa
    16801 cggatggttc ttcgcatcca gccggatcga gttgaagggg tcgcccgacg gttcgacacc
    16861 gatctcgctt tccacccggt ccgccccgag cgtatccagc gccaacgcga atacaaacgg
    16921 cttcgagatc gactggatgg tgaagggcac ccccgtatcg ccgacttcat aaacgtggcc
    16981 gtcgagggtg gcgaggctga tgccgaaatg ctcagggttg gccttggaca gttcgggaat
    17041 gtactgcgca acctcgccgc catgatcaga ggtgaaatcg gcatggcagg cggtcaggaa
    17101 gcgctgcagc ggcggcattg cggcgctcca gttgccggcg tgcgaaaccg gcgtctgtcg
    17161 catggagttc atgtgtagtg tgtcctgccg tgtgatcggg cgctttggcg caatgttacg
    17221 tcttggctga ggaggccgaa accggcggaa gcatgttttg tgaggccgag ggagtttgtg
    17281 ccggagcggt tgacgccggg cgttcacgtt tcgatgatcg ccacaacctg gccttcggca
    17341 accatgtcgt cgagggcaac gagcaacgac ttcaggatgc cggctgccgg agatgcaacg
    17401 ggaatttcca tcttcatggc ctcgacaaag gcgatgtcgt cgccgtcgcc gacacttgcg
    17461 ccggtctgaa ggggaagtgc acacacgcgg ccggcaactt cggtcacaac gctgatatct
    17521 ggcataccaa ttggcttctt gttgtggcgg cagattgcct gaggtagctt gttctgcaag
    17581 tggaatttta ttccatctac cggaaatacg gagagcccat gggaagacgc tcggagcggc
    17641 tgagcaggca tggcatgctt gccggcgatg caggcgaggg cgacgtcatt caggtggtgt
    17701 cgcgcgcatt cgacgtgctg cgatgcttcg agggccacga agcacgactc ggcaatctcg
    17761 agatttccaa tcgctgtgga ttgccgcgat cgacggtgtc gcgactgacc cacacgctga
    17821 cgcggatggg ccaactggtg tatctgccgc gcgaccagaa ataccgcatt ggtccaagcg
    17881 cagtcgccat gagcacctca atgatgaagg ggctgcagct tcgcaatctg atcaagctgc
    17941 gactgcaaga cgtcgccgag cacttgccgg ggacggtcgg tttcgttatc cccgaccgct
    18001 tccatctcgt ttacctcgaa tatgcgcgcg ccgcgaatgc gctcggcctg cacgacgtga
    18061 ccggcagccg catcgcaatg gcgaccacgg ccgccggcca tgcttatacc gcggcgctcg
    18121 cgccggagat tggcgatgct ctgcttgccg aaatggagcg ggaaatcccg gatgtcgcga
    18181 aggtgctgca gcctcgcatc gagaacaatc gccgcttcct gagagagcac ggttatgtgg
    18241 tcgcctgcgg tctctggagc ccacatatca atgggctcgc ggtgccgctc tggtcgccgc
    18301 agtaccagac ctttgtagtg gtgacgatcg ggctgctcgc cgcgatgtac gacgagaggc
    18361 ggttgcacag ggaggtggcg ccgccgatga tcgaactcgg gcgtgccgtc ggggagttgt
    18421 tcgaaggcgc cgaaggcgat ctcttcaaca accgtctgga gcgcaagtcg cttccggtac
    18481 ggaccaacaa taacaagatc atcaagacgg aggagacgaa tgatctggaa gcccgaactc
    18541 gacgacctcg ctcggcgcga agccttcgcg cgcgagatgg gcggcgttga gaaggtcaag
    18601 cggcagcgcg accagggccg gctcaccgtc cgcgaacgca tcgacaaact ggtcgatgcc
    18661 aacagcttcc acgaagtcgg cgcgatctcc ggaatcggcg aatacgacga acacggcgag
    18721 ctcaagcatc tgacgccggc caactgcgtg ttcggtcgcg ccagggtcaa cggccggacc
    18781 gtggtcgtgg tcggcgacga ttttaccgtg cgcggcggct cggcagatgc gtcgatctcg
    18841 gccaagccgc tgatggcgga agagatggcg cacgactttc gcctgccgat catccgcatc
    18901 atcgaaggtt ccggcggcgg aggttcggtc aagacgatcg agaccaaggg tgcggccaat
    18961 cttcccggcg gtattggcgg cacgcgctgg taccgttaca cgacggaaaa tctcgcgcat
    19021 gtgcccgtcg tcgctctggg attgggatcg gtcgctggcc ttggcgcggc acggcttgca
    19081 gccagccact attcgatcat gaccagggac tccgcgatgt tcgtcgccgg cccgccggtg
    19141 gtgaagcggc tcggacagga tctttccaaa cagaagctcg gcggcgccga catccagacc
    19201 agagccggcg gtgtggacga cgccgtcgac accgaggagg aggcatttga gaaggcaaag
    19261 cgtttcctct catatctgcc ttcatccgtc ttcgaactgc cccccacgct gccctgcgat
    19321 gatgatcccg aacgaagcga ggaaatactg atgaaggcgg tgccgcgcag ccgccgccag
    19381 gtctacaaga tgcgcccgat catcgaggcc gtcgtcgaca agggctcgtt tttcgagatg
    19441 aacgccaatt tcggccgccc gatg
//
LOCUS       DATLJT010001270         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_98692,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001270 DATLJT010000000
VERSION     DATLJT010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98692"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VM910_29200"
     CDS             complement(<1..554)
                     /locus_tag="VM910_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVI66623.1"
                     /translation="MGAHVEVMDLVAQMKADEQIFVLATVVRTVSVTAAKAGAKAIIR
                     PDGTIVAGWIGGGCAKGAVLKAAREALADGEPRMVSVRPENLLAELGVKPGETREGVR
                     FASNMCPSKGTMDIFVEPVLPHPSLVILGASPVALSLAAQARPLGYHVTLAAPAVDLA
                     GTPDVDVLIGGFALGELHDAKRFVV"
     gene            complement(557..1765)
                     /locus_tag="VM910_29205"
     CDS             complement(557..1765)
                     /locus_tag="VM910_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI66624.1"
                     /translation="MSSCDPTPGFDELEEVSRLVSRKLASFLRTLRDAGFAVGLAEGR
                     DAATLMAAGYAARPLLLRSALKHLVSARKSDWEKFDGLFDAFWLGKRVRARSLTSGSA
                     KAANNPSLKSLRDTSAPQGGRGSASDQVPSNDEAPEGRSGEGRMEGASRAENLGEIDF
                     RKLADPDQVERAHDAAARLARAMRTRLTRRDLARRRGYRLDLRRTIHRNISHGGVPIS
                     RVMRRRKDKPLRLVVLLDASGSMSLYTGFFLRFIHGVLDEFREAEAFLFHTRLAYVSS
                     AMKEKDAGRALDRLSLLAQGTGGGTKIGESLKTFNRHHASRVIHSRSCVMIVSDGYET
                     GDADLLGREMAALSKRCRRIVWLNPMMGWEGYAPEAKGIKAALPYVDLYAPANTLNSL
                     AALEPYLARI"
     gene            complement(1762..2634)
                     /locus_tag="VM910_29210"
     CDS             complement(1762..2634)
                     /locus_tag="VM910_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVI66625.1"
                     /translation="MKNRDEIAQALARSGYIADSELATAISLMQLLKRPLLLEGEAGV
                     GKTEVAKALASVHATELIRLQCYEGLDQSSALYEWNYQRQLLAIQAHRGENADAVEDR
                     IFSEKYLLERPLLAAIRRDKPPVLLIDEIDRADDEFEAFLLELLSDFQVSIPELGTIA
                     ATTIPHVVLTSNGTRELSDALRRRCLYHYVDYPDVDREARIIVARIDGASTSLSLQIA
                     QMIAGIRKEDLRKVPGVAETLDWAAALVGLDVKDLQEVPELVHETLMCLVKTHEDRAK
                     LTREVADRLLGKVA"
     gene            complement(2729..>3884)
                     /locus_tag="VM910_29215"
     CDS             complement(2729..>3884)
                     /locus_tag="VM910_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI66626.1"
                     /translation="GQFPYHSALGWEYDSGNYDVALRQAMQAVDYGGLRREQAEKREA
                     FRRGETREIMGLGVSFFTEIVGAGPSKNCDILGIAMFDSCEIRMHPTGAGIARMGTKS
                     QGQGHETTWAQIIASEIGIPADNIMVEEGNTDTAPYGLGTYGSRSTPVAGAAIAMAAR
                     KIKAKAQMIAAYKLEVHEDDLEWDIDGFRVKGLPEKFMSMKDICWAAYNSVPPGMEPG
                     LEAVSYYDPPNMTYPFGAYLCVMDIDVDTGVYKVRRFYALDDCGTRINPMIIEGQVHG
                     GLTEAFAIAMGQEIRYDEGGNVVTGSFMDFFMPTAVETPHWETDFTVTPSPHHPIGAK
                     GVGESPNVGGVPAFSNAVNDAFSFLGSTHIQMPHDYWRNWKAAKALGAVA"
BASE COUNT          683 a         1300 c         1188 g          713 t
ORIGIN      
        1 acgacaaagc gttttgcgtc atgcagttcg ccaagcgcaa aaccgccgat gagcacgtcg
       61 acgtccggcg taccggcaag gtcaactgcc ggtgcggcga gcgtgacgtg atagccgagc
      121 gggcgtgctt gcgcggcaag cgacagcgcg accgggctcg cgccaaggat cacaagcgag
      181 gggtgtggca gcaccggctc gacgaaaatg tccatggtgc ccttgctcgg gcacatgttg
      241 gacgcaaatc gcacaccctc cctggtttcc cccggcttga cgccgagttc ggcgagcagg
      301 ttttccggcc gcaccgagac catgcgcggc tcgccgtcgg caagcgcctc gcgcgctgcc
      361 ttcaaaaccg cgccttttgc gcagccgccg ccgatccagc ccgcaacgat cgtgccgtcg
      421 ggacggatga tcgctttggc gccggctttt gccgctgtta ccgagacggt ccgcacgacg
      481 gttgccagca cgaatatctg ttcgtcggcc ttcatctgcg cgaccagatc catcacctcg
      541 acatgggcac ccatctctag atcctcgcca gatacggttc gagcgcggcc aggctgttca
      601 gcgtattggc cggcgcgtaa agatcgacat agggcagcgc cgccttgatg cccttggcct
      661 cgggcgcata gccctcccac cccatcatcg gattgagcca gacgatgcgg cggcagcgtt
      721 tgctcagcgc cgccatctcg cggccgagca gatcggcgtc gcccgtttca tagccgtcgg
      781 acacgatcat cacgcagctt cgcgaatgga tcacgcgcga cgcatggtgg cggttgaacg
      841 tcttgaggct ctcgccgatt ttcgtgccgc cgcctgtgcc ctgcgccagc agcgacagac
      901 ggtcgagtgc acggccggca tctttttcct tcatcgcgga cgagacgtac gccagccgcg
      961 tatggaacag gaaggcttcg gcctcgcgaa attcatccag cacgccgtgg atgaagcgga
     1021 ggaagaagcc ggtgtaaaga ctcatcgagc ccgaggcatc gagcagcaca acgaggcgca
     1081 gcggcttgtc tttgcgtcgg cgcatcaccc gcgagatcgg cacaccgcca tggctgatgt
     1141 tgcgatggat ggtccgcctg agatcgagcc gatagccgcg ccggcgcgca aggtcgcgcc
     1201 gcgtcaggcg tgtgcgcatc gcgcgtgcca gccgcgcggc cgcatcatgc gctcgctcca
     1261 cctggtcggg gtcggccaat ttgcggaaat cgatctcgcc gagattttcg gcgcgcgagg
     1321 cgccttccat ccgaccctct ccgctgcggc cctccggcgc ctcgtcgttg gagggaacct
     1381 gatcgctcgc ggacccgcgt ccgccttgcg gggcgctcgt gtcccgcagg cttttcagtg
     1441 acggattgtt cgccgcttta gccgatccgc tcgtcagtga ccgcgcacgc acccgctttc
     1501 cgagccagaa cgcgtcaaac agcccgtcga atttctccca gtcggatttc cgcgcggaga
     1561 ccagatgttt cagcgccgag cgcagcagaa gcggccgcgc cgcgtagccc gcagccatca
     1621 gggttgcggc atcgcgtccc tcggccagcc ccaccgcgaa gccggcatcg cgcagcgtgc
     1681 gcaggaagga ggcgagcttt cgcgacacca aacgcgagac ctcttccagt tcgtcaaagc
     1741 cgggtgtcgg atcgcaggag ctcatgcgac tttccccaac agtcgatcgg cgacctcacg
     1801 cgtcagcttc gcgcgatcct catgcgtttt caccaggcac atcaatgtct cgtgcacgag
     1861 ttcggggact tcctgcaggt ctttcacgtc aagaccaacc agcgccgcgg cccaatccag
     1921 cgtctcggcg acgcccggca ccttgcgcaa atcctctttc cggatacccg cgatcatctg
     1981 cgcgatctgc agcgacagcg atgtggaggc gccatcgatc cgcgccacga tgatgcgcgc
     2041 ctcgcgatcg acgtcgggat agtcgacata atgatacagg caacgtcggc gcagcgcatc
     2101 ggaaagttcg cgcgtaccgt tcgaagtcag gaccacatgg ggaatggtcg tcgcggcgat
     2161 cgtcccgagc tccggaatcg aaacctggaa gtctgacaag agttcgagca aaaatgcctc
     2221 gaattcgtcg tcggcgcggt cgatctcgtc gatcaacagt accggcggct tgtcgcgccg
     2281 gattgccgca agcagcggcc gctccagcag atatttctcc gaaaagatcc ggtcctcgac
     2341 ggcgtcggcg ttctcgccgc ggtgcgcctg gatcgcgagc aattgccgct ggtaattcca
     2401 ctcgtagagc gccgaggact gatcgagccc ctcgtagcac tgcaggcgga tcagctcggt
     2461 tgcgtgcacg gacgccagcg ctttcgcgac ctcggtcttg ccgacgcctg cttcgccttc
     2521 cagcagaagc gggcgcttga ggagctgcat cagggaaatc gccgttgcaa gctcgctgtc
     2581 ggcgatatag ccggaccgag ccaacgcttg cgcgatctcg tcgcggtttt tcatattcat
     2641 gctccgtcac cgcgagcgaa gcgacgcaat ccgggccaac gcgacggctc gggattgctt
     2701 cgtcgcttcg ctcctcgcga tgacgacact atgccaccgc gcccagggcc ttcgccgcct
     2761 tccagttgcg ccagtaatcg tgcggcatct ggatgtgggt cgagcccagg aacgagaagg
     2821 cgtcgttgac ggcattggag aacgccggca cgccgccgac attcggactc tcgccgacac
     2881 ccttggcgcc gatcggatga tgcggcgacg gagtgacggt gaagtcggtc tcccaatgcg
     2941 gggtctccac cgcggtcggc atgaagaaat ccatgaacga accggtgacg acgttgccgc
     3001 cttcgtcata gcggatttcc tggcccatcg cgatcgcaaa ggcctccgtc aggccgccat
     3061 ggacctgccc ctcgatgatc atcggattga tgcgggtgcc gcaatcatcc agcgcataga
     3121 agcgccgaac cttgtacacg ccggtgtcga cgtcgatgtc catcacgcag agataggcgc
     3181 cgaacggata ggtcatgttg ggcggatcgt aatagctcac cgcttcaagt cccggctcca
     3241 tgcccggcgg cacggaatta tacgccgccc agcaaatgtc cttcatcgac atgaacttct
     3301 cgggcagccc cttcaccctg aagccgtcga tgtcccattc gaggtcgtcc tcgtggacct
     3361 cgagcttgta ggctgcgatc atctgcgcct tcgccttgat cttgcgcgcc gccatcgcga
     3421 tcgcagctcc cgcgaccggc gtcgagcgcg agccgtaggt gccgagcccg tagggcgcgg
     3481 tgtcggtatt gccttcctcg accatgatgt tgtcggcggg gatgccgatt tcgctggcaa
     3541 taatctgggc ccaggtggtc tcgtgtccct ggccctggct cttggtgccc atgcgcgcga
     3601 taccggcgcc cgtcgggtgc atgcggatct cgcaagaatc gaacatcgca atccccaaga
     3661 tgtcgcaatt cttcgacggg ccggcgccga cgatctcggt gaagaaggag acccctagcc
     3721 ccatgatctc gcgcgtctcg ccccgcctga aggcctcgcg cttctcggct tgctcgcggc
     3781 gcaacccgcc atagtcgacg gcctgcatcg cctggcgcag cgccacgtcg taattgccgg
     3841 aatcatattc ccagccgagc gccgaatgat acgggaattg cccg
//
LOCUS       DATLJT010001271         7690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4234812,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001271 DATLJT010000000
VERSION     DATLJT010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234812"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            654..998
                     /locus_tag="VM910_29220"
     CDS             654..998
                     /locus_tag="VM910_29220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66627.1"
                     /translation="MKTANLFHAGPSMSTINDLDPDALISRLAGPLSPADRAAFRAAA
                     EDALARVPCWGEGAIYRAVASLQRAFRNPPSDRNAHWDIEHELRDTKLKSAAPIEYGG
                     DGRHTRYRKHAR"
     gene            1008..1496
                     /locus_tag="VM910_29225"
     CDS             1008..1496
                     /locus_tag="VM910_29225"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HVI66628.1"
                     /translation="MYWCCAQIEQCHDRLAWHCLTLAGYKVYQPLLREQRRSHGRKII
                     ATPPLFPGYLFVWVVKGWWDARWAAGVRHLVMDGEAPARVPDAVIAEIKSRERNGFVE
                     LPRPRGFVPGMRVKVVSGPLQNQIGMLAILRPHERVLVLLRLLGGEQRVELARTAIEP
                     IE"
     gene            1508..1681
                     /locus_tag="VM910_29230"
     CDS             1508..1681
                     /locus_tag="VM910_29230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66629.1"
                     /translation="MSKDSERQAWTKFVEEREREAKRPRAKKRAKEEASQAELEAGLD
                     DLVTDADRRGAKR"
     gene            complement(2003..2212)
                     /locus_tag="VM910_29235"
     CDS             complement(2003..2212)
                     /locus_tag="VM910_29235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66630.1"
                     /translation="MSKGKRDYRRQYQLAKQRWHYQMVRLDHEDAQMLKDVAEQKRTT
                     VVELIRTYVAWGLMEDDSARRPDDL"
     gene            complement(2467..2679)
                     /locus_tag="VM910_29240"
     CDS             complement(2467..2679)
                     /locus_tag="VM910_29240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66631.1"
                     /translation="MTSSQDDDDDDDDFLTKEDCLIEIVRGDDDDRHWDALTRFVELG
                     GTQEEMRAAFDKWIALLERALKGGRH"
     gene            complement(2676..3320)
                     /locus_tag="VM910_29245"
     CDS             complement(2676..3320)
                     /locus_tag="VM910_29245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66632.1"
                     /translation="MPNDRPRMIIVLATESPSDAIWGRIRMLADEIFAGPVAVKFAYY
                     GREGTGATRRPCIATNWITVADDWTDIVDQGRVDCICGCFMQTDDFLTQALRETQKTT
                     VQAVVVLGSFHGKLDTAVATTKLLRVAGTRVFCFQQDRSSSTERAFRALAEIGGGAFY
                     RINPHVDLIAERLPRLMQAVTHYATGGMAALEAQGDEPANLLLEQMTAADQTLL"
     gene            complement(3383..4969)
                     /locus_tag="VM910_29250"
     CDS             complement(3383..4969)
                     /locus_tag="VM910_29250"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HVI66633.1"
                     /translation="MTDDKIVKLPIPAAQQAKGADEHARADTERNQRLFDWADAVLKQ
                     LGLHKAVADAKSIETLRGVTFNADSAEIAMAIRDALHPASGKRAEYFRGLREGSLKQI
                     LKNRFTELKKAREAELRRRRSKDWTDDLILDENGRIKPILANLVLILREGPDWKSALA
                     YDEFSARVVIRKSPPLEKAALTSWIDVPWADHHESQTRVWFQRRSINPTLGDVGRAVQ
                     VAARHNPFHPVRDYLNALNWGGLSRLETWLQTYLHVEDSEYVRAIGPRYLISAVARIF
                     MPGCQCDHMLILEGPQGKQKSKALRELAIRDEWFADRLSHVGTKDASIETAGVWIIEI
                     AEMEGLVRATPSAQKRFVSARHDRYRPPHGKHTINRPRQNVFAGTINPPTTGGYLKDP
                     TGARRYWPVTCIGTIDVVGLAQVRDQLWAEAVHLFKAGMPWWLETPALESLATTEQAK
                     RFAADPWEEPIREWLEDRIVVTLWDVMQYALSLNRDQQQTQSAQKRVKNILTTRLGFT
                     YTRPRTPEGRTPCYRRDPPR"
     gene            complement(5224..5484)
                     /locus_tag="VM910_29255"
     CDS             complement(5224..5484)
                     /locus_tag="VM910_29255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66634.1"
                     /translation="MARAKSSASESIITVPVRPRALFQRVNRALLADGKELRRTRGEP
                     AIRALGALYVASGNGVIDHHVNLEMLARKLGVIEPYEHLIKD"
     gene            5889..6179
                     /locus_tag="VM910_29260"
     CDS             5889..6179
                     /locus_tag="VM910_29260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66635.1"
                     /translation="MVRKQRAEQWAEHRRRHDEEERRQRVMVITEFGTEDPEATMHAL
                     AKEILRYRQALRLLATAIGWASSGMPFGLFPPGPFRRSRRTIEGTDDAPPAA"
     gene            7023..7370
                     /locus_tag="VM910_29265"
     CDS             7023..7370
                     /locus_tag="VM910_29265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66636.1"
                     /translation="MSGNARVGTPASVGGEPGGTSGSIHGLGYLFSALRREGLIADEL
                     VLQLATFLRSLELFLELTVRTVPRTLTTNQVRRGGKQGTDDPELSRIHSRSQHPRDKR
                     REASARGAAYRST"
     gene            complement(7355..>7690)
                     /locus_tag="VM910_29270"
     CDS             complement(7355..>7690)
                     /locus_tag="VM910_29270"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HVI66637.1"
                     /translation="FAYRKKIVPGCTCNGRDAFGLVNTAVADDPTLRPGDIVATNTGL
                     MAYSAGPNGSTFTPISAYAGLSADLRRQLTETKIEPAAEEPAPLPPVREAEATSARST
                     KNKRAQVDR"
BASE COUNT         1418 a         2250 c         2385 g         1637 t
ORIGIN      
        1 agttcgaacc gagtctcttg gatcagaaca tggatattga aaattcgcga ccagagactg
       61 acgcacgaaa tcaacctgat ggcgctcgaa atcgagacaa aatcgccctc ggagactagc
      121 cagcgatagc taacccgcga gaatgtcgca caaatttttg tgagctagac ctaacccaca
      181 gagactggac tggctggctg ggcatgtaag actcgaactc agaaatgtca tcgcaaaata
      241 tccctttgaa aggtggcaca gatttccagg gatccagccg aattctggcc acagagacta
      301 ttcgcgtttg agctgcggcg ttgaggacat gcagctcggg cttagggcgc cggggcaaga
      361 gcaggcgcgg atgcagtcct gcttgcggtg aaagtcacgc cactcttcta agcctcgaga
      421 cgaagtcgtc agggtcattg cgcgactcct tcgggcaggc gtggtacccg ccactggcca
      481 gatggcggac ccgctgccgg aagcggcccg tgcgccctca cccccagacc gagggcgagg
      541 gccgtgctag gcagaagcgg ccagtatatc tatcagttgc gtgcgaatcg aggtccgccg
      601 tacaaagaac ggcccgccgg gcagcgggcc gagtctctga cgaatcgcac tgtgtgaaga
      661 cggccaattt attccacgcg gggccgtcta tgtccacaat caacgatctc gacccggatg
      721 cgctgatctc acgtcttgcc ggtccattat caccggctga tcgcgccgct tttcgcgccg
      781 ccgccgagga tgctctcgca cgtgtccctt gctggggtga gggcgccatt tatcgagcgg
      841 tggcatcgtt gcagcgtgcc ttccgcaatc cgcccagtga ccggaatgcg cattgggaca
      901 tcgaacatga actgcgggac accaagctca agagcgctgc gccaatcgag tacggcggcg
      961 atggccgcca tacccgctac cgcaagcacg cgcgttgaga gcgcgctgtg tattggtgct
     1021 gcgcccaaat cgagcagtgt catgatcgtc tggcgtggca ttgcctcacg ctggccgggt
     1081 acaaggtcta tcagccgttg ttgcgcgagc agcgtcgtag tcacggccgc aagatcattg
     1141 ccacgccgcc gctgttcccg ggttatctgt tcgtttgggt ggtcaaaggc tggtgggatg
     1201 cgcgatgggc ggcgggggtg cgccatctcg tcatggacgg tgaggcaccg gcgcgcgtcc
     1261 ccgatgctgt cattgctgag atcaaatcga gagaacgtaa tggcttcgtc gagttgccgc
     1321 ggccgcgcgg attcgtacca ggtatgcggg tgaaggtggt tagcggtccg ctgcaaaatc
     1381 agattgggat gctcgcgatt ctgcgccccc atgaacgtgt actcgtgctt ttgcgtttgc
     1441 ttggcgggga gcagcgcgtc gagctcgcaa ggactgcgat cgagcctatc gagtagcggc
     1501 ggcagacatg agcaaggaca gcgagcgtca agcgtggacc aagtttgttg aagagcgcga
     1561 gcgcgaagca aagcgaccgc gagccaagaa gcgagccaaa gaggaagcgt cgcaggcgga
     1621 gctcgaggct gggctcgatg atcttgtcac cgatgcggat cgcaggggcg cgaaacggtg
     1681 agggtcgaat tggctaggta atccggtcca gaccaccgag caaaatgcac cagtatctca
     1741 tcaccgcttt ttcgcgattc cgcgctggcg cgcccagaag ggggtgacgc ccgccggcgt
     1801 tgcaataatt ggtcaatcct ggcctggacc ttccaatctg tccggctgtt cgcgatgtgc
     1861 caattcccgc aaaggcggca acgatacggc tccatgccct tgttcttgag gcggcgaatg
     1921 tgaatcatcg cagcgtttcg tgttttgtgg gtgactttac cgctggagca cattgctctg
     1981 cctcttcctc ctcctacatt tctcaaagat cgtcgggacg acgtgcactg tcgtcctcca
     2041 tcagtcccca cgcgacgtat gtgcgaatca actccacgac cgtggtgcgt ttctgctcgg
     2101 cgacgtcttt cagcatctga gcgtcctcgt ggtcgagccg gaccatctgg taatgccagc
     2161 gttgtttggc gagttggtat tgccgccgat agtccctttt tcctttgctc aagaacgtag
     2221 ctccctctag ttcctgcgac gaagggaggc gacgcgttta caaagtcggc tttggtttcg
     2281 cctttccgat ccgacgtacc gtggcagttc ggatgggatc atcggaggct ctccaaactt
     2341 ggagttcaga aactttatcg ggaagcgccg ccgcgagctt ttgtaaggcg tgtccaaggc
     2401 tgttggcctc gataaactgg ggttctgcga tgtccccgtt gtctttaaga acgtagataa
     2461 atctcattag tgcctgcctc cctttagcgc gcgttccaag agtgcaatcc acttgtcgaa
     2521 cgcggcgcgc atttcttctt gcgtaccgcc caactcgaca aatctggtta aggcgtccca
     2581 gtgccggtcg tcatcgtcgc cgcgcactat ttcgatcaga caatcttcct tcgtcaggaa
     2641 gtcatcgtcg tcgtcatcat cgtcttggct actggtcata gtaacgtctg gtcagccgcg
     2701 gtcatttgct caagcagcag attggcgggc tcatcgccct gtgcttccaa tgcggccatg
     2761 ccgccggttg cataatgcgt gaccgcctgc atcaagcgcg gcaagcgctc ggcgatcagg
     2821 tcgacgtgtg gattgatccg atagaacgcg ccgccgccga tttcggcgag tgctctgaat
     2881 gcgcgctcgg tcgagcttga gcggtcctgc tgaaagcaga atacgcgggt gccagcaacg
     2941 cgcaggagct tggtggtcgc gaccgcggtg tcgagcttgc cgtgaaagct accgagaacg
     3001 acgaccgctt gaacggtcgt tttctgggtt tcgcgcaggg cctgggtcaa aaagtcatcg
     3061 gtctgcatga aacagccgca gatgcaatcg acacgcccct gatcgacgat atcggtccag
     3121 tcgtcggcga ctgtgatcca gttcgtcgca atgcagggcc gccgggttgc acccgtgccc
     3181 tcgcgcccgt agtaggcaaa cttgacggcg acaggcccgg caaaaatctc gtctgcaagc
     3241 atcctgatcc ggccccaaat ggcatcgctc gggctctcgg tagcgagtac gataatcatg
     3301 cgtggtcgat cgttaggcat tgttgggttc cttctggtcg acgactggtc aggcgtggtc
     3361 agccgtcagt ttcacatctt ttttagcggg gagggtctct ccgatagcat ggcgtgcgtc
     3421 cctcgggcgt gcgtggccgg gtgtatgtga agcccaagcg cgtcgtcagg atattcttga
     3481 cccgcttctg ggcggactgc gtctgctgct gatcgcggtt gagactgagg gcgtactgca
     3541 tcacgtccca gagagtgacg acgatgcgat cctctagcca ttcgcgaata ggttcctccc
     3601 agggatcggc agcgaagcgt ttggcctgct cggtcgttgc gagcgattcg agtgctggcg
     3661 tctccagcca ccacggcatg ccggccttga ataaatgcac cgcctcggcc cacaattgat
     3721 cgcgcacctg tgccaagcca acgacgtcga tcgtgccgat gcaggtgacc ggccaatatc
     3781 ttcgcgctcc cgtcgggtcc ttcaggtaac cgcccgttgt cggtgggttg atggtgccag
     3841 cgaatacatt ttgtcgtggt ctgttgattg tgtgcttgcc atgcggcggg cggtagcgat
     3901 cgtgacgagc agacacgaat cgtttttgcg ctgacggtgt agccctcact aagccttcca
     3961 tctcggcaat ctcgatgatc caaacgcccg cggtttcgat agaggcatcc ttggtgccga
     4021 cgtgactgag ccggtcagcg aaccactcat cgcggatggc aagctctcgt aatgctttgg
     4081 acttctgttt gccttgcggg ccttcgagga tgagcatgtg gtcgcactgg cagcctggca
     4141 tgaagatgcg agcaactgct gagatcaggt atcgcggtcc gatggcgcga acgtattcgc
     4201 tatcttcgac gtgcaggtat gtttggagcc aggtctccag acgcgacagt ccgccccagt
     4261 tgagcgcatt gagataatcc cgcaccggat gaaacggatt atggcgagcc gctacttgca
     4321 ccgcgcgccc gacatcgcca agcgtcggat tgatgcttct gcgttgaaac caaactcgtg
     4381 tctgggactc gtgatgatcg gcccagggca catcgatcca agacgtgagt gccgccttct
     4441 ccaacggtgg agacttcctg atgacgacgc gagcactgaa ttcatcgtag gcgagcgcgc
     4501 tcttccagtc cggcccctcc cgcaggatca agactaggtt cgcgaggata ggcttgatcc
     4561 taccgttctc gtcgaggatc aagtcgtcag tccagtcctt gctgcgcctg cgccgcagtt
     4621 ccgcttctcg ggccttcttc aactcggtga agcggttctt caaaatctgc ttgaggctac
     4681 cttcgcggag accacggaaa tactccgcgc gcttgccgct cgcgggatgg agcgcatcgc
     4741 gaatcgccat ggctatctcg gcgctgtccg cattgaacgt aacgccgcgc agcgtttcga
     4801 tggactttgc gtcggccact gccttgtgca ggccgagctg tttgagcacc gcatcggccc
     4861 agtcgaaaag ccgctgattg cgctctgtat cggcgcgcgc gtgctcgtcc gcgcccttag
     4921 cttgttgcgc cgctggaatc ggcagcttca cgatcttgtc gtcggtcatg cccaaccccg
     4981 cgcgcagaaa aagttggggc aaatacggct gaccacggca gaccagccgc ttaaccgggc
     5041 tgcaaggatg gagacattgc cagcgtcagt cgtctcgttg gtgtcggtgg tgatgtccgc
     5101 caacggaatg tcgcggatgg gatgaggatt ggccgcagct gcggctttcg ccgatgctgg
     5161 cgatctgcta actgccatcg ttgtaatcca caacttggga gcatgctggc tcccattttt
     5221 ttcttagtct ttgatcagat gctcgtaggg ttcgatcacg ccgagttttc tcgcgagcat
     5281 ttccaagttc acatgatggt cgatgacgcc gttgccgctg gcgacgtaaa gagcaccgag
     5341 cgcgcggatt gccggttcgc cgcgcgtccg ccgcaattct ttcccgtcgg ccagcaacgc
     5401 cctgttgacg cgctgaaaca gcgcgcgcgg tcgcaccggc acggtgatga tggattctga
     5461 agcagaagac ttagcacggg ccacggctat cccctttgcg gatcacgact gtccgctttg
     5521 tggaaagggg agcttaaagc ctggcctggg gtcgattgaa ccggacgtgc cgatctccgg
     5581 catccggctc tccgactggt cgaccgaggt cgagagatat cgacctcacc gtctagggta
     5641 gtcttcttgt tagctcttgc tctgacgttc ttatgcgcgt gagcgcgcat catgtcaacg
     5701 ggcagattcg tcgggagtga aaatgggtgc gtcgtcagcg cagccccttc gccgccttca
     5761 ccagctcacg cccgcgttcg agcgtctcgt cgatgcgcct ggggagattc ggttgcgctc
     5821 ctgggagcac actcggtgac cgaagtagaa cactgatgct gccatgttca atcggaggat
     5881 tgctgcagat ggtcaggaaa caacgcgcag agcaatgggc cgagcatcgg cgccgacacg
     5941 acgaagagga gcggcgccaa cgtgtgatgg tcatcacaga gttcggcacc gaggatcctg
     6001 aggcgacgat gcatgccctg gccaaagaga tcctgaggta ccgccaagcc ctgcggctgc
     6061 tggcaacggc gatcggctgg gcgagctcgg gtatgccgtt cgggctgttc cctcccggtc
     6121 ctttccggcg ttctaggcgt accattgagg gtactgacga cgcgccacct gcggcatagg
     6181 ctgtgggcgc gatgatgaaa tcaagcgata gacaggcaac ggccgagctc ctggccgccc
     6241 tcgtcgaggc agcgatcgcg cacgggcgcc ggcattacag ccagagcggt gaacagttgg
     6301 cgatgatcgc ttgcggtgct cgagtgcctg cagcgggagg ggtttgtgac cgtcccggcg
     6361 acctaaatct tgcccggtag gcgctcgagc acgtagcatc cgcggcgtac caacaagggt
     6421 acagctttcg ggctatgcac gcggcctcgg cgggatcacc actcgcgccg gggccgctgc
     6481 gtttctgggc gaccctcgac tgcgcctagg gggcatcgca gccgctcgcc cggagacgcg
     6541 acccgtcgag tctcgccaaa atggttcgct ttggctcgct tggttcttag ctgcttccta
     6601 tgtgtttcct aagcccaatt ctaggaagcg gccctgctag gaagtgccat caattcggca
     6661 cttctttgcg cgttagccct ctttctgcga taacgcgatt aagatgccga gatcatagct
     6721 ccggggtggt cgctgtcatc ggcaagacga tgctctcaaa gctgggcgtt ctctgatctc
     6781 tgacccgcat ggaacagcgt ctcggccggg ggcgcctccc aggcgaggga ggccggcggc
     6841 aggcaaagac gccagcgtga gcgcgaggcc gacggccgct gaaagacggc attatcaaat
     6901 cacaagcctt ggcagcaact gggtttcgct tggtgatttc gaggcgccca tgcattgccg
     6961 ggcggccccg ataagcagca gcggcaccgt cccccgatct tgaggcgctc gcggagtcat
     7021 ctttgagcgg caacgcgcgg gtgggaacac cagcgtcagt gggcggggag ccgggcggta
     7081 catccggctc gatccatggt cttggctatc tcttcagcgc gctcaggcgt gaggggctga
     7141 tcgccgacga gcttgtcctg caactggcga cattcctccg ctcgcttgag ctgtttctgg
     7201 aattgacggt gcgcactgtc ccgcgaactc tcacaaccaa tcaggttcgg cgtggcggca
     7261 agcagggcac agatgatccc gagctttcgc gcattcattc ccgttcccag catccacgcg
     7321 acaaacggcg ggaagcctca gcacgtggcg ccgcttaccg gtcgacctga gcccgcttgt
     7381 tcttcgtgct gcgggcggat gtcgcctctg cctcccggac gggtggcagt ggtgcaggtt
     7441 cctccgcggc cggctcgatt ttggtttcgg tgagctggcg gcgcaagtct gctgagagtc
     7501 ccgcataggc gctgatggga gtgaacgtcg agccgtttgg cccggcgcta taggccatca
     7561 gaccagtatt ggtcgcgacg atatccccgg gacgcaatgt cggatcatca gcaacggctg
     7621 tgttgacgag gccgaaggcg tccctgccgt tgcaggtgca gccggggacg atcttcttcc
     7681 ggtacgcgaa
//
LOCUS       DATLJT010001272         3937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1709426,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001272 DATLJT010000000
VERSION     DATLJT010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709426"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VM910_29275"
     CDS             <1..696
                     /locus_tag="VM910_29275"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HVI66638.1"
                     /translation="ALGGLTTHPLKDGTWLSLKITDFTLALHVGGGSTHVQSFSLDGR
                     PDAQARQWLSEQLGARGLNPHALDAPSPYEMPAHAVSQGAAYGPAGLSDALAELAAWF
                     GNAGLSLASIQRQMIGRKLAASPARCWPHHFDLATLTTLPARNADATGYVGAGFSPGD
                     EYYDEPYFYVSVYPEPDPTALPSLPTLGHWHLRDFVAAVATAHKIVAAKNQQAETDEF
                     LRAAVDGAIKVFS"
     gene            complement(912..1373)
                     /locus_tag="VM910_29280"
     CDS             complement(912..1373)
                     /locus_tag="VM910_29280"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI66639.1"
                     /translation="MSVEGLILHHGEAHGVTLHQTRIDYLITAEHSKQCSLFEFTVAP
                     GFNTGAHYHTKIEEIFYVLEGELDLRCGERTVRGGPGTCVFVPPSTAHAFGNSGSKPG
                     RMLLITAPPGHEKYFDELRDLVAKGGKPDPDALAKLRAKYDTVQLATLTPG"
     gene            complement(2463..3446)
                     /locus_tag="VM910_29285"
     CDS             complement(2463..3446)
                     /locus_tag="VM910_29285"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66640.1"
                     /translation="MIITRRHFNALAATSAFMSSIAANRATALAWPIRPVHLVVPFAA
                     GGPTDVIARIVGERLSKGWGQQVLIENRPGGGTNIANEMVVRSEPDGHTVLMGGSSQA
                     TARGLYRSLSYDPITDFAPVAFICSYSFFMFVPNSLPVKSVSEFITYARANKDKLTLA
                     SPGTGSSPHLCGELFKHMAGLNMVHVPYRGAAPAMNDLIPGRVHLLFSGGATLDNARS
                     GQVQVLGYTGAKRSAIAPEVPTIAEDGVPGFNVVSWYGFFVPVKTSTEIIVKMNTDII
                     TALADPSVQGRLAPLGYESRPDAPEQVGAFLKADAQLWGRVIKEAGIAPQD"
     gene            complement(3490..3783)
                     /locus_tag="VM910_29290"
                     /pseudo
     CDS             complement(3490..3783)
                     /locus_tag="VM910_29290"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          794 a         1155 c         1187 g          801 t
ORIGIN      
        1 gcccttggcg gccttaccac gcatccgttg aaagacggca cgtggctgag cttgaaaatt
       61 accgatttca cgctcgcatt gcatgtcggc gggggaagca cgcacgtcca atccttttct
      121 ctcgacggcc gcccggatgc acaggcacgc cagtggctga gcgagcagct cggcgcacga
      181 ggactcaatc cgcacgctct tgacgcgcct tcaccctacg agatgcccgc gcacgccgtc
      241 tcccaaggcg cagcctatgg cccggccggc ttgagcgatg cattggccga gctcgcggca
      301 tggttcggca atgccggact ttcgctcgcc tctatccaac gacaaatgat tgggcgcaaa
      361 ttggcggcct cgcccgcgcg ctgctggccg caccacttcg atctcgctac tttgaccaca
      421 ctcccggcgc ggaacgctga cgccaccgga tatgtcggcg ccgggttttc tcccggagac
      481 gagtattacg acgagcccta tttctatgta tcggtctatc ccgagcccga tcctacggcg
      541 ctgccgagcc tgccaacgct cggtcattgg catctgcgcg atttcgtggc ggccgttgcg
      601 acagctcata agatcgttgc ggccaagaac caacaggccg agaccgatga gttcctgcgg
      661 gccgcggtcg acggcgcgat caaggtcttc agctgaagct attcgcgatg ggccaggacg
      721 agcttcagcg gcccagcggt tgcgtggtct cgatcagtga gacgaagcga tttcatcacg
      781 ttgctcggcg gtgtggccgc tggcggcgtg cgcgctgcag ggcgaccggg tgcggcgcat
      841 cggcgtgctc ataagccttg ccgcggggcc ttaagcgttc tcagcgtcgg attgcagctt
      901 actggatcac ttcagcccgg tgttagtgta gcgagctgga ctgtgtcgta ctttgcgcgc
      961 agcttagcga gcgcatcggg atccggcttg ccgcccttag cgaccaggtc gcgtaactcg
     1021 tcgaagtact tctcatgacc tggcggcgca gtgatcagca gcatgcggcc gggctttgaa
     1081 ccggagtttc cgaacgcgtg cgctgtgcta ggcggaacaa atacgcaagt tcccggcccg
     1141 ccgcgcacgg tacgctcgcc acagcgcaga tcgagctctc cttcgagaac gtaaaagatc
     1201 tcctcgattt tggtgtgata atgcgcacca gtattgaatc ctggggcgac cgtaaactca
     1261 aagagcgagc actgcttgga atgttcggcc gtgatcagat aatcgatccg agtctgatgc
     1321 aacgtaacac cgtgagcctc accgtggtgc agaatgagtc cctctacgga catctgcgcc
     1381 tcctgttcgc atagacgcgg ggaaggttat gggagtgttc tcatgagggc aacctggcac
     1441 tcatactact gaaaactcac cgcatccgtt gctgacagtt cggtatggac cggctgctgt
     1501 cgctgcaatt gtttgaacag aatttccgca tttataagtg aagcctctgc cgggagttag
     1561 gccgccgctg gcccggatgc cgttatggtc ttagacgacc cactgctgcg ccctacgcct
     1621 tatgtggggg ggacgggcgg tcgcgccccc tcgctcatgt ggcgtcaccg cctcccctgt
     1681 cgacggggag ggatgcgatc ctgggcgccc tgcgaacttc tccgccagca tcacgtcggc
     1741 acgctcacgc gcttacccgt tcccgctcag catggcgatc agagcaatcg cggcgagcga
     1801 gaaccaaata aatcagcgaa tcaaatgcac gctcgattgc gccggggcct gggggcgttg
     1861 cgcctcgcct ttgttgtcgt cagtcatgtt cttaacccct tttgtcaacg tagctaacgg
     1921 cgatgagcta aagattgacg ctgccgtaga tgatgagccc acccaagatg atgaggaaca
     1981 gaaccgcagc ggtgagcggt ccccaatcat cttcagggcg ccatgggtcg ggccaccgac
     2041 cgtagtggcc gctagaagag cgaccttggt cttcaaagcg tgggtctgtc atattggtac
     2101 ccgcgtttag tcagcgtgga gttgtgatcg tcaccgctct tcgcacttgt agctgcggcg
     2161 aaggaaagcc agcgtcgttc cagagcaagg aagtccacct tcatgagagg atggctggca
     2221 gcttcggcta tccgacgagc cgcagcagcg cggaggtagc attcccaatt aggtgacctg
     2281 aaggtgtgag gcatgatgac ccgtaatctc cgagggatcg cccggtgacg atgggtcggt
     2341 gagggaggta tcctggttca aaacctccag attaaatcga tgtcatgtgc gcccgaatgc
     2401 cgccttcgtt gcgactttct gtcggggatc aggatagccg caacgtgcgg aaagaactgt
     2461 gatcagtcct gcggagcaat accggcttcc ttaattaccc ggccccacaa ttgggcgtcc
     2521 gccttgagga atgccccaac ctgctcggga gcatcgggcc tgctctcgta accgagtggc
     2581 gccaggcgtc cttggacgct cggatccgca agcgcggtaa tgatgtcggt attcattttc
     2641 acgataattt cagttgaggt cttaaccggc acaaagaagc cgtaccatga cacgacattg
     2701 aagccgggaa cgccgtcttc ggcgatggtt ggcacctcgg gcgcgatcgc ggaccgcttc
     2761 gcacccgtat aaccaagtac ctgtacctgg cctgatctgg cgttatcgag cgtggcgccg
     2821 cccgaaaaga ggagatgcac gcgaccggga atgaggtcgt tcatcgctgg ggccgccccg
     2881 cgataaggca cgtgcaccat gtttagaccc gccatgtgct tgaacagttc gccacaaagg
     2941 tgtggcgaag atccggtgcc gggcgaagcg agcgtgagct tgtctttgtt tgccctggcg
     3001 taggtgatga attcgctgac cgacttcacc ggcagagagt tcggcacgaa catgaaaaac
     3061 gagtaactac agatgaaggc gaccggtgcg aaatcggtaa ttgggtcata gctgagcgag
     3121 cgatagaggc cacgggccgt cgcctgcgac gagccaccca tcaacaccgt atggccgtcg
     3181 ggttctgagc gtacgaccat ttcgttggcg atgttggtgc cgccgcctgg ccggttctcg
     3241 atcaagacct gttggcccca gccttttgag agccgttcgc cgacaatccg ggcaatcacg
     3301 tcagtcgggc ctccggctgc aaacggcacg acgagatgga cgggacggat cggccaagcg
     3361 agggcggttg cccgattggc cgcaatgctc gacataaacg cagatgtggc agctagggcg
     3421 ttgaaatggc ggcgagtgat gatcacgaca tgtctccagc agtgcgacaa agttggcggg
     3481 ttgtccgacc tacaacgcca aagcctcttg cggatcgact tctgacgact cggcgcacag
     3541 cgaagagagc gacacaccga taagccgaac tcctttccgc agcggaaact gagcggcgag
     3601 taactccgcg ctgattgatg ccagcgtcga gcggtccaaa attaacgtga cacgtgatcg
     3661 actacgggtg atgatttgaa agtcagagaa cttcacttta agcgttaccg ttcatccccg
     3721 cacacctgta ctgtcgcaat ggtgccagac cttgtcgacc agcggaccaa gctcggctct
     3781 catgtcgtcc aggctggtca gatctcgcgg gaacgtgttc tccgctccaa tcgatttgcg
     3841 gatgcgattc gggagaaccg gacgatgatc aatacctcgg cagatccagt agaaatgccg
     3901 tcccgccttc ccgaaatgct tcaacaggaa ggtttcg
//
LOCUS       DATLJT010001273         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1938719,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001273 DATLJT010000000
VERSION     DATLJT010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938719"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VM910_29295"
     CDS             <1..985
                     /locus_tag="VM910_29295"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI66641.1"
                     /translation="TAAVPPEQTAPMPPERAPAIFWPDAAADLVAYVLFPKGNDRFWA
                     HGYDSMVAAAFAASDADDPRVKRGRPAGNQVSDAASPATAPLTSADLCGSTSATAAAD
                     ALIERIERLTEPSASQRDVLEQLRAALVQAIERIDATCPALVPATFTERLNAVQDRIW
                     IMHDALLTLRLPFENFYNSLTDEQQRRLRRDEPKSGESGASVTDGRAQTCAEPAAGIA
                     DGIMDAIGRAAPPSEPQRAGVEALRLNSAAMAKLIASSCPTDPLLGPMGRFAAATDRL
                     DVMLFAVMSMSPALQQLYNSLDDKQKAGLNRALRQARRSGPISEQPRTSQP"
     gene            complement(1314..2000)
                     /locus_tag="VM910_29300"
     CDS             complement(1314..2000)
                     /locus_tag="VM910_29300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66642.1"
                     /translation="MMGRPYGSGRVTCESCISIDVRRWHREGRLRPGLFFSWSWTCNG
                     EPFGDIKVRTEADAVVLMYRARSYGGAEWKSVEQRVPITWTACHLGGHRPWFICSVYS
                     NGRYCGRRVALLYIAGELFACRRCYGLAYASQHEALHHRGVGKAQKIRMRLGGSPNMR
                     EAFPDKPKGMHWRTYDRLRRMHDVAEGRSAVDLMRLVERLERRFARLTGADSANDFDN
                     SGGEESDHDY"
BASE COUNT          413 a          770 c          690 g          455 t
ORIGIN      
        1 gacggcagcg gtacctcccg agcagacggc gccgatgcct cccgagcgcg cgccagcgat
       61 attttggccc gatgccgccg ctgatctcgt tgcatatgtg ttgttcccga agggcaacga
      121 ccgcttttgg gcacacggct atgactccat ggtggccgcc gcattcgcgg cttcggacgc
      181 cgatgatccg agggtcaagc gcggccggcc cgccggcaac caggtcagcg acgcggcgtc
      241 gccggcaacg gcgcccctca cgtccgccga cctctgcggt agtacgtcgg cgaccgccgc
      301 cgccgatgca ttgatcgagc ggatcgaacg attaaccgag ccgagtgcat cgcaacggga
      361 tgtgcttgaa cagctgcggg ctgccttggt gcaggcgatc gaacggatcg acgccacttg
      421 ccccgccctc gtgcccgcga ctttcacgga acggctcaat gccgtccagg atcggatctg
      481 gatcatgcac gatgcgctcc tcacgcttcg cctcccgttc gagaacttct acaactcgct
      541 cactgacgaa cagcagcggc gcttgcgtcg cgatgagccg aagtccgggg agagtggagc
      601 gagtgtcacc gacgggcgcg cccaaacctg cgccgagccg gcggcgggaa ttgcggacgg
      661 gatcatggat gcgattggac gcgcggcgcc accgagcgaa ccgcagcgcg cgggcgtgga
      721 agcgttgcgg ctgaactcgg ccgccatggc gaagcttatt gccagttcct gccccaccga
      781 tccgctgctg ggccccatgg gtcgcttcgc cgctgcaacg gatcgcctcg acgtcatgct
      841 gtttgcggtg atgagcatga gcccggcgct gcaacagctc tacaattcgc tcgacgacaa
      901 gcagaaggct ggtctcaacc gggcgctgcg ccaagccagg cgctctggcc ccatcagcga
      961 gcaaccgcgg acctcgcaac cctgaacgtg gccggctatg tcgagggcgc aaacctcgtc
     1021 atcgagtacc gctgggcgga ggaaaactac gcgcggcttc ccgctctggc agccgacttg
     1081 gttcgttccg cttgttgggc gtgccggctg cccggccgct tctgtgctct cctggtctag
     1141 acccgcgcgg catctttgct acttttgcgg tccagtgata cggtaggcca tctcgccgtt
     1201 ggcattgatc ccaacggtca cgaaattgcc gcgcctgacc tcctcgtcca tcatcgcccg
     1261 aagcatcgcg tcccactcgt cgggctcacg aatttcggga cttcgctttc gtttcagtaa
     1321 tcatgatctg actcctcacc tccagaatta tcgaaatcat ttgcagaatc tgcgcctgtt
     1381 aggcgcgcaa atcgtcgttc caatcgttct acaaggcgca tcaggtcgac ggctgagcgt
     1441 ccttcggcaa cgtcatgcat gcggcgtaag cggtcatagg tccgccaatg catgcctttc
     1501 ggcttatccg ggaacgcttc ccgcatgttc gggctgcctc ccagccgcat tcggatcttc
     1561 tgtgcctttc cgacgccacg atggtgtaag gcttcgtgtt ggctcgcata cgccaggccg
     1621 tagcagcgtc ggcatgcgaa caattcaccg gcaatgtaga gcagcgcgac gcgacgcccg
     1681 caatatcgac cattggagta aacggaacag ataaaccagg ggcgatgacc gccgaggtgg
     1741 caggccgtcc aggtgatcgg tacgcgctgt tcgacggatt tccactcggc gcctccgtag
     1801 cttcgcgcgc ggtacatcaa aaccacggcg tccgcttcgg tgcggacctt gatgtcgccg
     1861 aacggctctc cgttacaggt ccacgaccac gaaaagaata ggcccggacg cagccgtccc
     1921 tcgcgatgcc agcggcgcac atcgatcgag atgcaggact cacaggttac gcggccacta
     1981 ccgtaaggcc ggcccatcat ctgacctcca gaattatcga aatcccttgg agaatctgcg
     2041 cgctttcagg cgcgcaaatg ttgcgagccg gcacccaatg gccggcgccg ggtgagcatg
     2101 acgcggacct cgcgctcggc ctcgtcaagc ttgacgagcg cccttgcgcc cttgggcaag
     2161 cttgttaggt gtgcccttct ggcggctgcc tcgacgctgt cccggttttg atccgcctct
     2221 aggcattggc tactttttca atccgacctt tgattttcct aacgaaacgc ccaaaaatct
     2281 tggctacttt ttgccgctct cgagcgccac cttcgcgatc gcccggat
//
LOCUS       DATLJT010001274         5623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3547109,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001274 DATLJT010000000
VERSION     DATLJT010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3547109"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..329)
                     /locus_tag="VM910_29305"
     CDS             complement(150..329)
                     /locus_tag="VM910_29305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66643.1"
                     /translation="MLSRNYLVQQVIGLLSLAKKTSDPDVTAALLKKATDLNDKLNST
                     PPKPDMSPRPPDVEH"
     gene            complement(470..1384)
                     /locus_tag="VM910_29310"
     CDS             complement(470..1384)
                     /locus_tag="VM910_29310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI66644.1"
                     /translation="MNAAPMQIAPEYRAAGTDLSERRRSGISSGPLIDIAGKADAAIT
                     WGTDGAATIGALTTIATIATDARIRHAYPGLAASAAGVATPQIRHLATLGGNIAQRSR
                     CWYFRNPHIDCLKKGGGDCPARAGNHLYGVAFDLGPCVAPHPSTMAAALSAHEARIST
                     DRRSTLTIETLLGDGRDGRSDHALEAGEMIRQVVLPVPLAGERALYKRAISRADAEWP
                     LVELCARAVIADGSFTFVRLAAGGIAPVPLRLLASEAALAGSPADAATIAHAARLAIS
                     GARPLPMTEYKLDLLSGLVRDLLEQLAV"
     gene            complement(1381..4068)
                     /locus_tag="VM910_29315"
     CDS             complement(1381..4068)
                     /locus_tag="VM910_29315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI66645.1"
                     /translation="MSTMTINGEQKPLPDDADALLADVIRDRLNLTGTKLVCGSGVCG
                     GCTVLLDGVPVASCLLPAKAASGKAVTTVEGIGGDRLHPIQKAFMANDALQCGFCTPG
                     FIVEAVAFHDRWRADKGTAMPSREEIAATLSGHLCRCGAYDNIFRAIEEACAGRFDGD
                     HVTSPRLEARDKVTGKARYTVDIRHDGQLEGMILRAELPHARITALDLAPARSLPGVA
                     AAISLLAEDKTVRYVGAPIAAVAAKDRRTALAALAAIGVTSETLPAVVGLDAARRADA
                     PVVFEKKDRKYAGNVSEGSNAPAPWKGNLRGPTGAFSQKPRRARSWLAKARAERNPLL
                     VENTFRVATQSHASLEPHAAVARFDGDELTVHVSTQAVHDIMGKISKRFGLAHDKVRV
                     IADHVGGGFGSKGSLGMETIAAIELARAAKAPVKVAYDREEELSVTGYRPAAEIEVAL
                     LPSAQGELKALSLTAYADTGAATSSTIAALARLIYPAQAKALTDYDVISNMPPGAPFR
                     GPGGPPMAFALEQAVDEAALRLNIDPISLRKRWDPDPNRQRLYAWAERLAVWRNRKQT
                     SAQSGRFRRGVGVAAGYWLYFWQPGSTVELAIKSGRIVARSAVQDIGTGTRSVIANAI
                     AKEFSLDPEEVEVRLGDSRFPEGPGSGGSRVTASIVPPTLAAVTKLKAEIIKAAPRLP
                     PEGSNAPWRDLIAATPDLAVTAARPKDDRPAPGVFSPLRQAGLMGMMFSFMLRTFNNM
                     VVGAGVPSSVQVVEVEVDTLLGRVRIVSAHTGLAVGRLAAPPLARSQAAGAVIQGIGY
                     ALYEARESDPFTGRVLSGSMDDYRIPGIADTPQLDVHFDEEGFDHVPGGSVGIGEIAT
                     VPTSPAIANAVRDAIGVRLTEIPLRTDRILAAVKGRTAA"
     gene            complement(4929..5561)
                     /locus_tag="VM910_29320"
     CDS             complement(4929..5561)
                     /locus_tag="VM910_29320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI66646.1"
                     /translation="MVAAEPAILQPAADEVNSKRRQILDGARKVFLDLGFDGASMGEI
                     ARSAGVSKGTLYVYFADKCRLFEAIVEDELAKGQLAFNFDPGRDLATTLRDCGKTHIA
                     MLCRPGGGSAIRTVMGIAERMPEVGRRYYKLALEQNINRLAAYLAEHVKTGELAIDDC
                     RLAAAQFPLMCQASLFLPWIFQAAPAPTPERIAEVIDSAVRMFLAAYQAK"
BASE COUNT          941 a         1767 c         1886 g         1029 t
ORIGIN      
        1 tcgggcgagg agatcgcctc gcgcgccgcg caccgcaatc cttatgcttc gcgtggaccg
       61 ttcgtcacgg gatcgctcgg cggccagcgg caggcgcccc gcaagagggc gcgcgagaag
      121 gtcgacgagg aagacgagtt cgaggacgat tagtgctcga catcgggcgg gcgcggactc
      181 atgtccggtt tcggcggagt gctgttgagc ttgtcgttca ggtcggtggc tttcttgagc
      241 aaggcagcgg tgacgtcagg atcgctggtc ttcttcgcga gcgacagaag accgatgacc
      301 tgctgtacga gatagttccg actcaacacg accggactcc attgttgttg ttctgttgtt
      361 gttgttgttc tcgcaagtgc aatacgcggg aacccggttt tccgttccgc agcgaaagtt
      421 caaaattgca cagaagcgaa ccgctctcgg ttccggatga cggttccacc tacaccgcga
      481 gctgctccag cagatcgcga acgagaccgc tcaacagatc gagcttgtat tccgtcatcg
      541 gcaatggcct agcgcctgaa attgccagcc tcgcggcgtg cgcgatcgtt gccgcgtcgg
      601 cggggctgcc cgccagcgcc gcttccgaag ccaggagtcg cagcggaacc ggtgcgatgc
      661 cgccggccgc gagtcgcacg aatgtaaagc tgccgtcggc gatcacggcg cgggcgcaga
      721 gctcgaccag cggccattcg gcgtcagcgc ggctgatcgc gcgcttatag agcgcgcgct
      781 cgccggcaag cggtacaggc agcacgacct gccggatcat ctcgccggct tcgagcgcgt
      841 ggtcggagcg gccgtcccta ccgtcgccga gcagcgtttc gatcgtgagg gtgctgcggc
      901 ggtccgtcga aatccttgcc tcatgggcgg agagcgccgc ggccatggtc gagggatgcg
      961 gtgcgacgca ggggccgaga tcgaacgcga cgccgtagag gtgattgcct gcgcgggccg
     1021 ggcaatcgcc gccgcccttc ttcaggcaat cgatgtgggg gttgcggaaa taccagcagc
     1081 gcgaacgctg ggcgatgttg ccgccgagcg tcgccagatg ccggatctgc ggggtggcaa
     1141 ctccggcggc gcttgcggcc aggccgggat aggcgtgcct gatacgcgca tcggtcgcga
     1201 tcgtcgcgat ggtcgtgagc gcgccgatgg tcgcggcgcc atcggtgccc caggtgattg
     1261 cggcatcggc cttgccggca atgtcgatca gcgggccgct ggagatgccg ctgcggcggc
     1321 gttcggacag atcggtgccg gcggcgcgat attccggcgc aatttgcatc ggcgcggcgt
     1381 tcatgcggcg gtccttcctt tcactgcggc gaggatgcga tcagttcgaa gcggaatctc
     1441 cgtgagccga acgccgattg cgtcgcgaac cgcgttggcg atcgcgggcg aagtcggaac
     1501 ggtcgcgatc tcgccgatgc cgacgctgcc gccgggcacg tggtcgaaac cttcctcgtc
     1561 gaaatgcacg tcaagctgcg gcgtgtcggc gattcccggg atgcggtaat cgtccatgct
     1621 gccggacagt acgcggccgg tgaacggatc ggactcgcgc gcctcgtaaa gcgcgtatcc
     1681 gatgccttga atcaccgcgc ccgcggcctg gctgcgcgca agcggcggcg ccgcaagtct
     1741 gccgaccgca agtccggtgt gtgcgctcac gatgcgcacg cggccaagca gcgtgtcgac
     1801 ctctacctcc acgacttgca ccgagctcgg cacgccggcg ccgaccacca tgttgttgaa
     1861 ggtgcgcagc atgaagctga acatcatgcc catcaggccg gcctgcctga gtggcgaaaa
     1921 aacgccgggc gcggggcgat cgtcctttgg ccgcgctgcg gtgacggcga gatctggagt
     1981 cgctgcgatc aggtcgcgcc acggcgcgtt cgagccttcc ggcggcagcc gcggggcggc
     2041 tttgatgatc tcggctttca gtttggtgac ggccgcaagc gtcggcggca cgatcgaggc
     2101 agtgacgcgg ctgccgcccg agcccggacc ttcgggaaac cgcgaatctc cgagacgcac
     2161 ctcgacctcc tcgggatcga gcgagaattc ctttgcgatc gcattggcga tcacgctgcg
     2221 cgtgccggtg ccgatatcct gcacggcgct gcgtgcgacg atgcggccgc tcttgatggc
     2281 gagttcaacg gtcgatccgg gttgccagaa atagagccag tagccggcag cgacgccgac
     2341 gccgcgtctg aagcggccgc tttgcgccga cgtctgcttg cggttgcgcc agaccgcaag
     2401 ccgttcggcc caggcgtaga gccgctgccg gttcggatcg ggatcccatc gcttgcgcaa
     2461 gctgatcgga tcgatgttga gtcgcagcgc cgcttcgtcg acggcctgct ccagcgcaaa
     2521 cgccatcggg ggaccgccgg gcccacgaaa cggcgcaccg ggcggcatat tgctgatcac
     2581 atcgtagtcg gtgagcgcct tggcctgggc cggataaatc aggcgcgcca gcgcggcgat
     2641 ggtcgagctg gtcgccgctc ctgtgtcggc ataggcggtg agcgagagcg ccttcagctc
     2701 gccttgcgcc gagggcaaca gggcgacttc gatctctgcg gccggtcgat agccggtgac
     2761 ggacagctct tcctcacggt cataggcgac tttcaccggc gctttcgcag cgcgcgccag
     2821 ctcgatcgct gcaatggtct ccatgccgag gctgcccttg gagccaaaac cgccgccgac
     2881 atgatcggcg atcacgcgca ccttgtcgtg cgcaagcccg aagcgcttgc tgatcttccc
     2941 catgatatcg tgcacggcct gcgtcgagac gtggacggtg agttcgtcgc cgtcgaatcg
     3001 cgcgacggct gcatgcggct cgaggctggc atgcgactgc gtcgccacgc ggaaagtgtt
     3061 ttcgaccaaa agcggattgc gctccgcgcg tgccttggcg agccatgacc tggcgcgtct
     3121 tggcttctgc gagaacgccc cggtcggccc gcgcagattt cccttccacg gcgccggtgc
     3181 gttgctgcct tcggagacgt tgccggcata tttgcgatcc ttcttttcaa acactacggg
     3241 cgcgtcggcg cgccgtgcgg cgtcgagccc gacgaccgcg ggcaatgtct cgctggtgac
     3301 cccgattgcg gcaagggcgg ccagcgcggt tcggcgatcc tttgcggcga cggccgcaat
     3361 cggcgcgccg acatagcgca cggtcttgtc ctcggcgagc agcgagatcg cggcggcaac
     3421 gccgggaagc gagcgcgccg gcgcaagatc gagcgcggtg attctggcat gcggcagctc
     3481 ggcgcgcagg atcatgcctt cgagctggcc gtcgtggcgg atgtcgacgg tgtaacgcgc
     3541 tttgccggtc accttgtcgc gtgcctcgag tcgcgggctc gtgacatggt cgccgtcgaa
     3601 acgaccggca catgcctctt cgatcgcgcg aaaaatgttg tcataggcgc cgcagcggca
     3661 gagatgcccc gagagcgttg cggcaatttc ctcgcgcgac ggcatcgccg tgcccttgtc
     3721 cgcgcgccag cggtcatgga acgcgacagc ttcgacgatg aagccgggcg tgcagaaccc
     3781 gcattgcagg gcatcgttcg ccatgaaggc cttctggatc gggtgcagcc tgtcgccgcc
     3841 gatgccctcc acggtggtca ccgcttttcc cgacgccgct tttgcgggca acaggcagct
     3901 tgcgaccggc acgccgtcga gcagcaccgt gcacccgccg cagacgcccg agccgcagac
     3961 gagctttgtg ccggtcaggt tcaaccggtc gcggatcacg tcggccagca gcgcgtcggc
     4021 atcgtccggc agcggctttt gctcgccgtt gatcgtcatg gtgctcatgt ccgcttctcc
     4081 ggtttacgcg ccttcgcgga tgctttcgcc cgcgcgtttt tgcgtctgcg atcggcgtcc
     4141 acagcatggt cgagatctct ctcagggatc gtcgagggac acttccttcg agacccgaca
     4201 gcgcgtctac gcgcgcttcc acctcaatca tacgcatggc gtcggcgata gccattgtgc
     4261 gtcatcatca tggcatcgtg atgggacgtc attgcacaag caaacggacg ccgtccaggc
     4321 cggttgacgc acgatcatgg tcaagcgcgg tgcgatcgcg ttccgccgca tgatttgccc
     4381 gacaaagcaa agcgaaaacc gtgcatgcgt gtcatgcgca aattgcccgt cgtgccaatc
     4441 tgccgcatgt gcgcggcgtt gtcgaaggac ccaaatcaac agcacatttg cgccatcccg
     4501 cctcgatgag agggacgtat cgcgatcgtc acgacacgtg gagcgcggga tgcgatggac
     4561 gcgaaggcat cgtcggacgt gcgatgcatt ttgcggacgg cgaagtcgtg tggtcctgac
     4621 gccccttggc tggcgttaag tctgtcgagg atgatcgaca ggcgacggtg actaaaaagt
     4681 catggtgagc acgaagcagc gttaacacca tcgggcaggg aatgccgtga tgatgcggcg
     4741 tacctgtggt gacttaagct tgtgtgcttc ttccattcgc acgcaaggct gcgggtgcag
     4801 cacaacgccc ggcattccct gcgccctctg tctttcgagg gacgtggttc tcgcaaaact
     4861 tcggacacgt cgtgtcgcga gaatgcgggg ttatgtccgg gctgtttgaa aaatgaatcg
     4921 gagccgcgct acttcgcttg atacgccgcc agaaacatcc gcaccgcact gtcgatcacc
     4981 tccgcgatgc gctcgggcgt cggggcaggg gccgcctgga agatccacgg caggaacagc
     5041 gacgcctgac acatcagcgg gaattgcgcg gccgcaagcc tgcagtcgtc gatcgcaagc
     5101 tcgccggtct tgacatgctc tgcgagataa gcggcgagac ggttgatgtt ctgttcgagc
     5161 gcaagcttgt aataacgacg gccgacctca ggcatccgct cggcaatccc catcacggtg
     5221 cggatcgccg atccgccgcc cggccggcac agcatcgcga tgtgggtctt tccgcagtcg
     5281 cgcaatgttg tcgcgagatc gcgcccggga tcgaagttga atgcgagctg ccccttcgcg
     5341 agttcgtcct cgacgatcgc ttcgaacagg cggcacttgt cggcgaagta gacataaagc
     5401 gtgcccttgg agacgccggc ggagcgggcg atctcgccca tgctggcgcc gtcgaagcca
     5461 aggtccagaa agaccttgcg ggcaccatcg agaatctggc ggcgcttcga attgacctcg
     5521 tctgcggcgg gctgaaggat cgcgggctcg gctgcaacca ttgattaagt gtctctggaa
     5581 ggatttggac aacggaaccg tctcgaaagg tccgcagtct agg
//
LOCUS       DATLJT010001275         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2397547,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001275 DATLJT010000000
VERSION     DATLJT010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397547"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VM910_29325"
     CDS             <1..947
                     /locus_tag="VM910_29325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66647.1"
                     /translation="LTLPLLAFLGFVAVCGTVVFSVAAPALVPSLVAPDQLPAANARI
                     ELARTVAFASGPALGGVLVGWIGAAPAFGVAAALSAVAVVLLSGIHEPARAAMPRRHP
                     LQEVKEGASFVLHHALLRPVFITQFTFNTGSFLTLAVFVPYAVRHLGLSATGVGATLA
                     MYGVGMVIGALSATRVMRRLAFGTVIGLGPVTGFAAAVVMALTTIIPTPLLAGMSFFM
                     LGVGPILWVISTTTLRQSVTPPRLLGRVTAINILSYGARPLGSALGAILGGLFGAEIC
                     LYVAVLIFAAQALVILLSPAVSLTRQPDMVGDIKAARA"
     gene            complement(956..2215)
                     /locus_tag="VM910_29330"
     CDS             complement(956..2215)
                     /locus_tag="VM910_29330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66648.1"
                     /translation="MELSTRIDLMNWLVAQGLTGLPENDLIRGFCERCRAGGLDLSRG
                     LAFIDTLHPIYEGRGFRWNDTATNEGDTFEYRSSSEGDAAKNWRRSAFHHMLENGHDE
                     LIIDLADCASLDFSMIGELADKGHRHFLAFVHRFGDAGTLGQMDCFYSYWTTRRAKGF
                     TESELAALRDLVPVLGLAIKSAQQVDIARTLGRVYLGRDASAQVLGGRISRGVTERIS
                     AVLWFSDLRGSTKVSESIGPDEIIPFLNDYAEASIEAIHDAGGDVLKLIGDGVLAIFT
                     GDDMTAARRAALSAEHRFRLNMIELNARRVAAGRPVTSAYVGLHVGEVFYGNIGSEDR
                     LDFTVVGPAVNEVSRIASMCSSVDREFLASAEFRTGLDATGRRYLVSTGRYALRGIGR
                     AQDLYTLDPDISASEAMTGSYERYLAS"
     gene            2367..>3328
                     /locus_tag="VM910_29335"
     CDS             2367..>3328
                     /locus_tag="VM910_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66649.1"
                     /translation="MSGRQLPVILALGTTQTLAWASSVYLPAILADPIARDLGISSNW
                     IFAAYSAALALAAVLGPQVGRQIDRVGGRPVLSASNMTLAAGLVLLGFANSIPMLMAA
                     WLVLGVGMGYGLYDAAFGVLGRIYGEAARGPITGITLIAGFASTVGWPLTALGVQTIG
                     WRDTCLAWAAAHLLIGLPLNMFMLPAIKHAKAAAETATKPHIPIDRSMILLAFIFAAA
                     WTVTGAMAAQLPRILEAAGATTLQAVSAGALIGPAQVVARIVEASVLKRYHPLLSTKL
                     ACLMHPLGAAIVAVFGGAAASVFAIFHGAGNGVITISRGTLPLA"
BASE COUNT          518 a         1128 c         1070 g          612 t
ORIGIN      
        1 ggctgacgct gccgctgctc gccttcctgg gcttcgtcgc ggtgtgcggc acggtggtct
       61 ttagcgttgc agcgcccgcg ctggtgccgt cgctggtcgc gccggatcaa ttgccggcgg
      121 ccaatgccag gatcgagctg gcgcgcaccg tcgcattcgc gagcggtccc gcgcttggcg
      181 gcgtgctggt cggatggatc ggggctgcgc ccgcgttcgg tgtcgccgcg gcgctctccg
      241 ccgttgcggt ggtcctgctg tcgggcatcc acgagcccgc gcgcgccgcg atgccgcgcc
      301 gtcatccgct gcaagaggtc aaagagggcg ccagcttcgt gctgcatcac gcgctgttac
      361 ggccggtctt catcacgcaa ttcactttca acaccggctc tttcctgacg ctggcggtgt
      421 tcgtgcccta tgcggttcgc catctcggcc tgtccgcgac cggcgtcggc gcgacgcttg
      481 cgatgtatgg cgtcggcatg gtgatcggcg cgctctccgc aacacgcgtg atgcgccgcc
      541 tcgccttcgg caccgtgatc gggctcggcc cggtcaccgg ctttgccgca gccgtcgtga
      601 tggcgctcac cacgatcatc cccacaccgc tgcttgccgg catgagcttc ttcatgctgg
      661 gtgtcgggcc gatcctctgg gtgatctcga ccacgaccct gcgccagtcg gtcacgccgc
      721 cgcggctgct cgggcgcgtg accgcgatca acatcctgag ctatggcgcg cgcccgctcg
      781 gctcagcact cggcgccatc ctcggcggcc tctttggtgc ggagatctgt ctctatgtcg
      841 cggtcctgat cttcgctgcc caggcgttgg tgatcctgct gtcgccggca gtctccttga
      901 cgcgtcagcc ggacatggtc ggcgacatca aggccgcgcg cgcctgacga ccaggctagc
      961 tcgccagata gcgctcgtaa cttcccgtca tcgcctcact ggctgatata tcgggatcga
     1021 gcgtgtagag atcctgcgca cggccgatgc cacgcagcgc gtagcggccg gtcgatacca
     1081 gatagcgccg gccggtcgcg tcgaggccag tgcgaaactc cgccgatgcc agaaattcgc
     1141 gatcgacgga actgcacatc gaggcgatac ggctgacctc gtttacggcg ggtccgacca
     1201 cggtgaagtc aagccggtcc tcgctcccga tattgccgta aaacacctcg ccgacatgca
     1261 gccccacata ggccgaggtg accggccggc ctgccgcgac gcggcgcgcg ttcagctcaa
     1321 tcatgttgag acgaaagcga tgctcggcgc tcagcgccgc tctcctggcc gccgtcatat
     1381 cgtcgccggt gaagatcgca agcacaccat cgccgatcag cttcaacaca tcgcccccgg
     1441 catcatggat cgcttcgatc gaggcttcgg catagtcgtt gaggaacgga atgatctcgt
     1501 cgggcccgat gctttccgaa accttggtcg agccgcgcaa atcggagaac cacagcaccg
     1561 cgctgatacg ttcggtgacg ccgcgggaaa tgcgtccgcc cagcacttgc gccgaggcgt
     1621 cgcgcccgag atagacgcgg ccgagcgtgc gcgcgatgtc gacctgctgc gccgacttga
     1681 tcgcaagccc aagcaccgga acgagatcgc gcaacgccgc gagttcggac tcggtaaaac
     1741 ccttggcgcg gcgcgtggtc cagtaggaat agaaacagtc catctggccg agcgtaccgg
     1801 cgtcgccgaa gcggtgcacg aaggccagga aatgccggtg ccccttgtcc gcgagttcgc
     1861 cgatcatcga gaaatcgagc gaggcgcagt cggcgagatc gatgatcagc tcgtcatggc
     1921 cgttctcgag catgtggtgg aaggcggagc ggcgccagtt cttcgccgca tcgccttcgc
     1981 tgctcgatcg atactcgaac gtgtcgccct cgttggtcgc cgtatcgttc cagcgaaatc
     2041 cgcgcccctc atagatcggg tgcagggtgt cgatgaaggc gaggccgcgc gacagatcga
     2101 ggccgccggc ccggcagcgt tcgcagaagc cgcgaatcag atcattttcg ggaagacccg
     2161 tgagcccctg ggcaaccagc cagttcatca ggtcgatgcg cgtggaaagt tccatccacc
     2221 aattatgccg cgcaatcccg cgaagcggaa gcttgaccca gcgtccggct tgacgctccc
     2281 ggcttgcggg atgcaaatgc caaaaagatg cgcacggcaa gacataacga cggcggccga
     2341 aggcgcgatc aagccacgat cgcggcatga gcggacggca acttcccgtc attctggcac
     2401 tcggcacaac ccagacgctg gcctgggcgt ccagtgtcta tttgccggct atcctggccg
     2461 atccgatcgc gcgcgacctc ggcatctcct cgaactggat ttttgcagcc tactccgcag
     2521 ccctcgcgct ggcggccgtg ctcggtccgc aggtcggacg ccagatcgac agggtcggcg
     2581 gccggccggt gctgtcggcg tcgaacatga ccttggctgc tgggcttgtc ctgctcggct
     2641 tcgcgaattc gattcccatg ctgatggcag cctggctcgt gctcggcgtc ggtatgggct
     2701 atggcctcta tgacgccgcc ttcggcgtgc tcgggcgcat ttacggcgag gccgcgcgcg
     2761 ggccgatcac cggcatcacg ctgattgcag gcttcgcttc caccgtcggc tggccgctga
     2821 cagcgctcgg cgtgcagacc atcggatggc gcgacacctg ccttgcctgg gcggccgcgc
     2881 acctcctgat tggcctgccc ctcaatatgt tcatgctgcc cgcgatcaag cacgccaagg
     2941 cggctgccga gaccgcgacc aagccccata ttccgatcga tcgcagcatg atcctgctgg
     3001 cctttatctt tgcggccgcc tggaccgtca caggcgcaat ggcagcgcaa ttgccgcgga
     3061 tcctggaggc ggcgggcgcc accacgctgc aggcggtgtc ggccggcgct ctgatcgggc
     3121 cggcgcaggt ggtggcgcgc attgtcgagg ccagcgtcct gaagcgctac catccgctgt
     3181 tgtcgaccaa gctcgcctgc ctgatgcacc cgctgggagc cgcgatcgtc gcggttttcg
     3241 gcggcgcagc ggcgagcgtg ttcgcgatct ttcatggcgc cggcaacggc gtgatcacga
     3301 tctcgcgcgg cacattgccg ctcgcgat
//
LOCUS       DATLJT010001276         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4235397,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001276 DATLJT010000000
VERSION     DATLJT010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235397"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VM910_29340"
     CDS             complement(<1..279)
                     /locus_tag="VM910_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66650.1"
                     /translation="MLLTRNSGSRLTAVLAACALLLALAGGARAGYPDRIIKIVVPFA
                     PGGGTDVVARTLAHEMAKDLGVSVIIENKPGAGTIIGTQSVATSDPDGY"
     gene            complement(280..1467)
                     /locus_tag="VM910_29345"
     CDS             complement(280..1467)
                     /locus_tag="VM910_29345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVI66651.1"
                     /translation="MQRKAEAKDQAVQAPVRIIALDRARTFITMLVVLHHSVVNYTHF
                     GSGDRMRWLGFDLIVLFNDSFFMACMFMISGLFVHDSLTRRGALSFIGNRAWRLGVPF
                     LVSIFVLMPIAYYPTFLRYHLPGTTDFNFFHYWWRTLTIGPWPSGPAWFLWVLLALDA
                     IAALLFVATPRAITGLGSAIYALRYRPGVAFALFLIFSVAVYLPMHLAFGDAAWLEPG
                     RYPLPIQTSRILLYAGYFLVGVGIGAADLRSGLVAQNSELAARWKIWLAIAAAFYGAI
                     LFLVYAHHNWVANFNAPPLSWRTGYGLAFALFSGAMAFAEPAFFLRFAGYPMRLLDVI
                     MPSAYGIFLVHYIFIIYLQYAVYEPALPALVKFAIVFAGTLSLSWATTVVLRKIPLVA
                     RMI"
     gene            complement(1481..2707)
                     /locus_tag="VM910_29350"
     CDS             complement(1481..2707)
                     /locus_tag="VM910_29350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI66652.1"
                     /translation="MAAIQLATAPAEAAAFGPPPGAELKLEKLDAIESFVDGEVAAGR
                     IPGAIVLIQQHGKRVYFKCFGKRDVDAGTPMTEDAIFPIHSVTKTITSVAAMMLVDRG
                     TIALDDPVSKYIPSFAGMKIGVERKDESGKTVLDPVALRRPITVEDLLLHTSGITYGF
                     YGVGLVKAAYDGIYLGDFDNAGFVERIAKVPLAEQPRTLWDYGHSMDVLGRVIEVASG
                     QSLYQFEKQNLLDPLGMTTTKFFLTDPGERARYAQPLAKDRHVERNSLNVTRWESGGG
                     GMVSTVADFERYGAMLLKGGTLDGRTYLSPATFKAMTTDHIGPGAGVARNYYYYPGDG
                     FGYGYGFGIRTDPGNASPPAPGSLGEIKWDGATGVYLVVDRAEDMFFVVMQDSPSGRM
                     HVITTIKKIVYDALEK"
     gene            complement(2879..3790)
                     /locus_tag="VM910_29355"
     CDS             complement(2879..3790)
                     /locus_tag="VM910_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI66653.1"
                     /translation="MDRLTSLTAFVRVVDSGGFSAAGRKLNMSTTMVSNHIQALEERL
                     GVRLLNRTTRKVSVTEVGRAYYDRCVQILSDIEQADDIAGALQSTPRGTLRIYTHTHM
                     VQFLAPVVNTFLGSYPDVKVDVTIGERTIDMVDEGYDIVVRMVPPPDSSLIVRSLATW
                     RHVLCCSHAYLEKHGRPQQLAELAERNCIRHVNYPYGDEWHFVDRKGAPASVRVSGNL
                     VTNSGEALHQAAVQGVGICLAAGFLIRDDLEAGRLVRLLPEYRTVEFSMNAVYPHRHH
                     LSAKVRTFIDMLAHHSSEQQKLINPYS"
BASE COUNT          811 a         1153 c         1217 g          679 t
ORIGIN      
        1 atagccgtcg ggatcgctgg tcgcgaccga ctgcgtgccg atgatggttc ccgcccccgg
       61 cttgttctcg atgatgacgg agacgccgag atctttcgcc atctcatggg ccagcgttcg
      121 cgccaccaca tcggtgccgc cacccggcgc aaagggcacc acgatcttga tgatccggtc
      181 cggatagccg gcgcgcgcgc cgcctgccag agcaagcagc agcgcgcatg cggcgagcac
      241 ggctgtcagc ctcgaaccgg aatttcgcgt cagcagcatt caaatcatcc gtgcaacaag
      301 cggaatcttt cgcaatacca ccgtggtcgc ccagctcaac gacaacgtgc cggcaaacac
      361 gatcgcgaat ttgacgaggg ccggcagcgc cggttcgtag acggcgtatt gcaggtagat
      421 gataaagatg tagtgcacca gaaagatgcc gtaggccgaa ggcattataa cgtcgagcaa
      481 gcgcatcggg tagccagcga agcgcaggaa gaacgccggc tccgcaaacg ccatagcgcc
      541 gctgaacagc gcaaacgcaa ggccgtagcc tgttcgccat gacagcggcg gcgcgttgaa
      601 attggccacc caattatggt gggcatagac caaaaacagg attgcgccat agaaggcggc
      661 ggcaatggcg agccagattt tccaccgcgc cgcaagctcg ctgttctgcg caaccaggcc
      721 cgatcgcaga tcggccgcac cgatgccgac gccaacgaga aaatagccgg catagagcag
      781 gatgcggctg gtctggatcg gcagcggata cctgccgggc tcgagccagg cggcatcgcc
      841 aaaggccagg tgcatcggca gatagactgc gacggagaaa atcaggaaga gcgcgaacgc
      901 gacgccgggc cggtagcgca aggcgtagat cgcgcttccg agccccgtga tggcgcgggg
      961 cgtggccaca aagagaaggg ctgcgatcgc atcgagcgcg agcagaaccc aaaggaacca
     1021 ggccggaccc gagggccagg gaccgatcgt gagcgtgcgc caccagtagt ggaagaaatt
     1081 gaagtccgta gtaccgggca gatgatagcg caggaaggtg gggtaatagg cgatcggcat
     1141 cagcacgaag atcgagacca ggaacggcac gcccagccgc caggcgcgat tgccgatgaa
     1201 gctcaaagcc ccgcgtcgcg tcaggctgtc gtgcacgaag aggccggaga tcatgaacat
     1261 gcaggccatg aagaagctgt cgttgaacag cacgatgagg tcgaagccga gccagcgcat
     1321 ccggtcgccg ctgccgaaat gggtataatt gacgacggag tgatgcagca ccacgagcat
     1381 cgtgatgaag gtccgcgccc ggtccagcgc gatgatgcgc accggcgcct gcacggcctg
     1441 atccttcgct tccgccttgc gctgcatcgg atcctttcga tcacttctcg agcgcgtcgt
     1501 agacgatctt cttgatcgtc gtgatgacat gcatccgccc cgagggggaa tcctgcatca
     1561 cgacaaaaaa catgtcctcg gcacgatcga ccacgagata gacgccggtc gcgccgtccc
     1621 atttgatttc gccgagcgaa cccggcgcgg gcgggctcgc gttgccggga tcggtgcgga
     1681 taccgaaacc ataaccgtat ccaaagccgt cgcccggata ataataataa ttgcgggcga
     1741 cgccggcccc cgggccaatg tgatcggtgg tcatggcctt gaaagtcgca ggactgagat
     1801 aggtccgccc gtccagcgtc ccgcctttca acagcatcgc gccgtagcgc tcgaaatcgg
     1861 caacggtcga aaccatgccg ccgcccccgg attcccagcg cgtcacgttg agggaattgc
     1921 gctcgacgtg gcggtctttc gcaagcggct gggcgtagcg cgcccgttca cccgggtcgg
     1981 tgaggaagaa tttcgttgtg gtcatgccca ggggatcgag caggttctgc ttttcgaatt
     2041 gatagagcga ctggccggag gcgacctcga tgacccggcc gagaacatcc atcgaatgcc
     2101 catagtccca gagcgtgcgc ggctgttcgg caagcggcac tttcgcaatc cgttcgacga
     2161 agccggcgtt gtcgaaatcg ccgagataga tgccgtcata ggctgccttc acgaggccaa
     2221 cgccatagaa cccgtaggtg atgccggacg tgtgcagcag gagatcctcg accgtgatcg
     2281 gacgacgcaa ggcgacgggg tcaagcacgg tcttgccgct ctcgtccttg cgctcgacgc
     2341 cgatcttcat cccggcaaag gaggggatgt atttgctgac aggatcgtcg agagcgatcg
     2401 tgccgcgatc gaccagcatc atcgcggcaa cgctcgtgat cgtcttggtg accgagtgga
     2461 tgggaaagat cgcgtcttcc gtcatcggcg tgccagcgtc cacgtcgcgt ttgccgaagc
     2521 atttgaaata gacgcgcttg ccgtgctgct ggatcagtac gatcgcgccc gggattcgcc
     2581 ctgcggcgac ctcgccatcg acgaaacttt cgatggcatc gagcttttcg agcttcagct
     2641 ccgcgcccgg cggcgggccg aacgctgccg cttccgcagg cgccgtcgcg agctgaattg
     2701 cggccaacag agcgatccgg ggtacgacga ggcgggtcat ccggagccga cgttattgta
     2761 ttgcggcgcg ttgatccaga tgacgcaccg ggtcattccg gaacagtccg acaggatccg
     2821 acccggaatc ccgagattcc cggttcgacg ttgacacgtc acgccgaaat gagcgctctc
     2881 aggaatacgg attgatcagc ttctgctgtt ccgaactgtg gtgagcgagc atgtcgatga
     2941 aggtgcggac cttcgccgac agatgatggc gatgcggata gaccgcgttc atcgagaatt
     3001 ccacggtccg atattcgggc aggagacgga ccaaccgccc cgcctcgaga tcgtcgcgaa
     3061 taaggaaccc tgcggcaagg caaatgccga caccttgtac ggccgcctgg tgcagcgcct
     3121 cgccgctgtt ggtgacaaga ttgcccgaca cccgcaccga cgccggcgca cccttgcggt
     3181 cgacgaaatg ccactcgtct ccataggggt aattgacatg acgaatgcaa ttgcgttcgg
     3241 caagctcggc gagttgctgc ggccggccat gcttttcgag gtaggcgtgc gagcagcaca
     3301 acacgtgtcg ccaggtcgca aggctgcgca cgatcaggct tgaatcgggc ggcgggacca
     3361 tgcgaaccac gatgtcatag ccctcgtcga ccatgtcgat ggttcgctcc cctatggtta
     3421 cgtcgacttt gacgtcgggg tagctgccga gaaatgtatt aacgaccggc gcgaggaact
     3481 gcaccatatg cgtgtgggtg tagatgcgca gcgttcctcg cggtgtcgat tgcagggccc
     3541 ccgcaatgtc gtcggcctgc tcgatatcgg aaaggatctg cacacagcga tcgtagtagg
     3601 cacgccccac ttcggtgacg ctgacctttc gcgtcgtgcg gttgagcagt cggacgccca
     3661 gacgttcttc cagtgcctgg atgtggttgc tcaccatggt ggtggacatg ttgagcttgc
     3721 ggccggcggc ggaaaatccg ccgctgtcca cgacccggac gaacgcggtc aggctggtca
     3781 accggtccat ggcttctcca attatccgtc tttactggac aatcctttta tttttgacgg
     3841 gattatcaca acagaggaga
//
LOCUS       DATLJT010001277         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1250308,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001277 DATLJT010000000
VERSION     DATLJT010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250308"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1543
                     /locus_tag="VM910_29360"
     CDS             164..1543
                     /locus_tag="VM910_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVI66654.1"
                     /translation="MMRKTSVILLSAATGAALTLLVTQPRAVFMGSSARAATSDTYRQ
                     LNLFGDVFERVRSDYVEKPDDSKLVESAISGMLAGLDPHSSYMDAKSFRDMQVQTRGE
                     FGGLGIEVTMEDGLIKVVSPIDDTPASKAGIMANDIITMLDDEQVQGLTLNQAVEKMR
                     GPVNTKIRLKIIRKGVDNPIEVTLVRDNIRVRSVRAHVEADDIGYIRITTFNEQTTEG
                     LKKEIANLQNQIGDKLKGFIIDLRNNPGGLLEEAVTVSDAFLERGEIVSTRGRNAEET
                     QRRSAHPGDLAKGKPVIVLINGGSASASEIVAGALQDHKRATLVGTRSFGKGSVQTII
                     PLGSGNGALRLTTARYYTPSGKSIQAKGIVPDIEVLQDVPDELKARTDTRGEASLRGH
                     LKSDGDEKTGSQSYVPPDAKDDKALKVADDLLHGIKSSATTPTPAMPATSDKAAADGP
                     QAVRPKAAN"
     gene            1686..2903
                     /locus_tag="VM910_29365"
     CDS             1686..2903
                     /locus_tag="VM910_29365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HVI66655.1"
                     /translation="MAETADDLSAPLGQKTVRRKRRVRLPFTAMQALAVLLGLFLVTF
                     AGFALFGHNPLGGEPIAHVAMRGTPAEDKAPVPPAVAGAHATPEAPKQAASGEARTVT
                     IIDGSSGKREDVVIGNASSDKADAGAKESDAAVPAITAGADPRLLERTRYGMIPVATA
                     DLKPFTAYAASADLAKAAKMPVVSIVIGGLGVGAAKTADAIMKLPGAVTLAFTPYGSD
                     PTKLAERARAQRHEILLQIPMEPYDYPDNDPGPQTLLATLGAEQNLDRMLWHLGRLQG
                     YAGIANFMGARFVATDAAMQPIIREAARRGLAYFDDGSAARSVAATLASSQAIPFAKG
                     DVAIDAVPTSAEVDHALAKLEDIAKERGYAVGTASALPLSIERIAAWTKGLEARGILL
                     VPLTTLMLKSKSS"
     gene            2950..>3269
                     /locus_tag="VM910_29370"
     CDS             2950..>3269
                     /locus_tag="VM910_29370"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HVI66656.1"
                     /translation="MARYEDLPYRTCVGVMLINKDGLVFIGRRAGGIEHVDESHIWQM
                     PQGGVDPGEDTWEAAKRELYEETSVRSVERLAEISDWLIYDIPRTVAGRAWKGRYRGQ
                     RQKW"
BASE COUNT          631 a         1075 c         1046 g          517 t
ORIGIN      
        1 ttacgggtga gccggtcgcg accatgggga cgagatccca ggttgcatcc attctcgccg
       61 ccaacgcgag ccaacccgtg ctctatatcg agttccgcaa ggacggcact ccaatcgatc
      121 caggcccatg gtgggccgca aatgaaggcg aaaaggttcg cggatgatgc gcaagacttc
      181 agtaattctc ctcagcgctg ccacgggtgc ggcattgacg ctgctcgtaa cccagccgcg
      241 cgcggtgttc atgggatcga gcgcgcgcgc tgcgacctcc gacacctatc gccagctcaa
      301 cctgttcggc gacgtattcg agcgcgtgcg cagcgactat gttgaaaagc cggacgatag
      361 caagctcgtc gaatccgcca tctccggcat gctggccggc cttgatccgc actccagcta
      421 catggatgcc aagagcttcc gcgacatgca ggtgcagacc cgcggcgagt tcggcgggct
      481 gggcatcgag gtgacgatgg aggacggcct gatcaaggtc gtctcgccga tcgacgacac
      541 gcccgcctcc aaggccggca tcatggccaa cgacatcatc accatgctcg acgacgagca
      601 ggtgcagggc ctgaccctca atcaggccgt cgagaagatg cgtgggccgg tcaacaccaa
      661 gatcaggctg aagatcatcc gcaaaggcgt cgacaatccg atcgaggtca cgctggtgcg
      721 tgacaatatt cgtgtccgct cggtgcgcgc gcatgtcgag gccgacgata tcggctacat
      781 ccgcatcacc accttcaacg agcagaccac cgaaggcctg aagaaggaga tcgccaacct
      841 gcagaaccag atcggcgaca agctgaaggg cttcatcatc gacctgcgca acaatcccgg
      901 cggcctcctc gaggaagccg tgacagtatc cgacgcgttc ctcgagcgtg gcgagatcgt
      961 ctcgacccgc ggccgcaacg ccgaggaaac ccagcgccgc tccgcccatc cgggcgatct
     1021 cgccaaaggc aagccggtga tcgtgctgat caacggcggc tcggcatcgg cctccgaaat
     1081 cgttgccggc gccctgcagg accacaagcg cgcgacgctg gtcggaaccc gctcgttcgg
     1141 caagggctcg gtgcagacca tcatcccgct cggcagcggc aacggcgcac tgcggctcac
     1201 caccgcgcgc tactacacgc cgtccggcaa gtcgatccag gccaagggca tcgtccccga
     1261 catcgaagtg ttgcaggacg tgccggacga gctgaaggcg cgaacggaca cccgcggcga
     1321 agcgtcgctg cgcggccatc tgaagtccga cggcgacgag aagaccggct cgcagtccta
     1381 tgtgccgccg gatgcaaagg acgacaaagc cctgaaggtc gccgatgacc tcctgcacgg
     1441 catcaagtcc agtgcgacga cgccgacgcc ggcaatgccg gcgaccagtg acaaggcggc
     1501 cgccgacggc ccgcaggcgg tacggcccaa ggctgcgaac tgattcggtt cgacccataa
     1561 agcgattcaa aaagggcggt ccccacgggg ccgccttttt acttgccggc tccggttgtc
     1621 cccatggcag ccgcgccggt cgcccgccct gtggtatcgt cagcggcgtt gattcgggac
     1681 ctttcatggc tgaaacggct gacgatctga gcgccccgct cggacagaaa acggtgcggc
     1741 gaaagcgccg agtccggttg ccgtttacgg ctatgcaggc gctggcggta ctgctcgggc
     1801 tgtttctggt cacgttcgcg ggctttgccc tctttggcca caatccgcta ggcggcgagc
     1861 cgattgcgca tgtcgcgatg cgcggcaccc cagcggagga caaggcccct gtcccgcccg
     1921 cagtggccgg ggcgcacgcc accccggaag cgccgaaaca ggcagcatcc ggcgaggcgc
     1981 gcacggtgac catcatcgac ggctcgagcg gcaagcgcga ggacgtcgtg atcggcaacg
     2041 cctcctccga taaagcggat gcgggcgcca aggagagcga tgccgcagtg cccgcgatca
     2101 cggccggcgc cgacccgcga cttctggagc ggacccgcta tggcatgatc ccggtcgcga
     2161 ccgccgacct gaagccgttc accgcctatg ccgcgtcggc cgacctggca aaggccgcaa
     2221 aaatgcccgt cgtgtccatc gtcatcggcg ggcttggcgt gggtgctgcg aaaacggccg
     2281 atgccatcat gaaactgccg ggcgcggtga cgctggcgtt cacaccctat ggctccgatc
     2341 cgaccaagct cgccgagcgg gcgcgtgcgc agcgccacga gatcctgctg cagattccga
     2401 tggagccgta cgattatccc gacaacgatc ccggaccgca gacactgctc gcgacgcttg
     2461 gcgccgagca gaatctcgac cgaatgctct ggcacctcgg ccgtctgcag ggttacgccg
     2521 ggatcgccaa cttcatgggc gcgcgctttg tcgcgaccga cgccgcgatg cagccgatca
     2581 tccgggaagc ggccaggcgt gggcttgcct atttcgacga cggttcggcg gcgcgtagcg
     2641 tggcggcgac gctcgcttcg agccaggcca ttccgttcgc caaaggcgat gtcgcgatcg
     2701 atgctgtgcc gacgtcggcc gaagtcgatc atgcactggc gaagctcgaa gatattgcga
     2761 aggagcgcgg ctatgcggtc ggcaccgcct cggcgctgcc cttatcgatc gaacgaatcg
     2821 cggcctggac aaagggattg gaggcccgcg gtatcctgct cgtgccattg acaaccttga
     2881 tgctgaaatc aaaatccagc tagtcggcac tggctttgcc ggatctgagc gcgaagaggc
     2941 cttcacggaa tggcgcgtta tgaggatctg ccctaccgga cctgcgtcgg cgtgatgctg
     3001 atcaacaagg acgggctggt cttcatcggc cgacgtgccg gcggcatcga gcatgtcgac
     3061 gaatcccata tctggcagat gccgcaaggt ggcgtcgatc ccggcgagga cacctgggaa
     3121 gcggccaagc gcgagctcta tgaggagacc agcgtccgct cggtggaaag gctcgccgag
     3181 atctccgact ggctgatcta cgacattccg cgcaccgtcg ccggccgcgc ctggaaggga
     3241 cgctatcgcg gccagcgtca gaaatggtt
//
LOCUS       DATLJT010001278         6365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2398207,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001278 DATLJT010000000
VERSION     DATLJT010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398207"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1548
                     /locus_tag="VM910_29375"
                     /pseudo
     CDS             1..1548
                     /locus_tag="VM910_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329566.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            1545..2369
                     /locus_tag="VM910_29380"
     CDS             1545..2369
                     /locus_tag="VM910_29380"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI66657.1"
                     /translation="MKAARFDYVRPGDVAEALAALAKAEGAKILAGGQSLGPMLNLRL
                     ARPALLVDISRLKALQQMEDTGRTWHIGAGVTHARLEDARGRLSGSEMLCEVAAAIAY
                     RSVRNRGTIGGSLAHADPAADWPLALAALGASVNIRGPTPRSIRVAHFIQGAFTTTLA
                     ANEIIESIVIPKLSRAGRYGFYKFCRKTGEFAEASAAAVFDPESGVANIFLGALRGTP
                     QSLASLAQQVAKHGGSAASTEFINQALAQSVGDLDAVERSMAAAAVTRALTQVLAS"
     gene            2366..3505
                     /locus_tag="VM910_29385"
     CDS             2366..3505
                     /locus_tag="VM910_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI66658.1"
                     /translation="MTPVALSVNGRKVQALVEPRTHLADFLREHCRLTGTHLGCEHGV
                     CGACTVLIDDKPARSCITYAVQCDGFAVQTIEGFEDDPIMQELRTAFSREHGLQCGFC
                     TSGMLIAARDIVQRLPDADAHRIRVELSGNLCRCTGYLGIINAVESVVAARRSKPMAV
                     TSAAAARPAPLHAFVPTGEVAPPPMTAPAPAVEEIRTGWTRFEESFVVAISPATVWAM
                     FADIAAVAACLDGAELTEHDANTAKGKMRIKLGPIQAGFSGSAVIERDDRAQRGIIRG
                     AGSDKGTGSRTKGEIVYRLTPEADGRQTRVSVTVEYSLQGPLAQFSRSGLVLALGRRL
                     VSDFAANLNAQLAGAKIRATPGVPFNAGRFLWSWFVDRLRRLFGR"
     gene            3633..5309
                     /locus_tag="VM910_29390"
     CDS             3633..5309
                     /locus_tag="VM910_29390"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI66659.1"
                     /translation="MAWQRAASLSEIDVDGVLGVDVNGSPVALYRLSNEVFATSGICT
                     HALALLSEGFVEDGKIECPLHQGQFDIRSGRALCAPATEDLCTYAVKLEGDDVFIDME
                     RPAASAQVVANAAPDRKAGGGVRAAEEDNKANTRKIAAMNMDPELSLTKRYVWPAEGL
                     TRIPDWVYTDQTIYEREIEKIFHGRTWNYVALECEVPKAGDFIRSNVGPTPVVVVRGD
                     DGSINVFENRCSHRAAEFCRELSGNVKEFVCPYHQWTYDLQGNLAGVPFRRGVNGKGG
                     MPADFDSAQHGLRRLNVTTHRGVVFASYVRDMESLQDYLGPEVLKEFEATFDGRKPRL
                     LGYYRHTLPGNWKLYHENLKDPYHATLLHTFLVTFGLLVAGNRSLMLADATGRHGVMA
                     SAKSERKSISSDAKKEMRAYRDGMTLAEPRFMDFIEEFDSPWSVTMATIWPNLIIQRE
                     MNTLGVRQIVPTGPHEFIMKWTMFGFEDDDDEMTRHRLRQGNLMGPAGFLGLEDNEAI
                     KFVQDGMQHVPGGQHLVKLDPAVASGTSDSLISEASIRAMYQHWRTEMGL"
     gene            5314..5853
                     /locus_tag="VM910_29395"
     CDS             5314..5853
                     /locus_tag="VM910_29395"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HVI66660.1"
                     /translation="MGEPALTRDHPPDAGSPDSRLQALLLHHEIESFNARYAQALDEQ
                     RLADWTEMFTDDALYVVLSRENFDRRLPVGLIYCENKRMIHDRAFALMETSMFAPRYL
                     RHIIANQVVVSADSNAMVKARANYVVLQVLFDRPDATLHQVGVYYDVFRRVGGELKLA
                     ERRCVYDNLLVPNALCVPV"
     gene            complement(5960..>6365)
                     /locus_tag="VM910_29400"
     CDS             complement(5960..>6365)
                     /locus_tag="VM910_29400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66661.1"
                     /translation="SVSAKDILDTGVDFGPDVIPVINTGWTEKTWGKPEFWEQMPYLE
                     PGVGELMVKKNVSAVAMDVFPEKAIWRGVKLDPGEVWVANHLALLEKGIPMIQFVTNL
                     SQIGKNRFVMIAAPLKIKGGDASPARVLALVE"
BASE COUNT         1170 a         1985 c         2022 g         1188 t
ORIGIN      
        1 gatcgccgcg agcatctggt ggtcggcgcc aactcacgac aacatcacta ccggctgacc
       61 gcccatgccg acgaacgcgg caggttgttg gcgctcgatg cggacatcac gatcgacggc
      121 ggcgcgtact cggcgtggcc gttcaccgtg gcgttggaac cgggacaggc aaccggcaac
      181 cttccaggac cttacgattt ccgcggctac cgctgccgga cccagtgcgt ggcgacgaac
      241 aagcccggct tcctgcccta tcgcggcgtc gcccggaccg gcgtttgttt cgccatggag
      301 ctggtgatcg acgcgatcgc gcgcgcggtc ggccgcgagc cgtgggaggt ccgctacgac
      361 aatctcgttt cggccgccga catgccctat gacaacgtgg cgcgcaaaca ttacgacagc
      421 ggcgactatc gcaaggcgct catccttgcg cgcgaaaaga tcggcctcga agcgtggcgc
      481 gagcggcaga gtcgcggaga gcgcgatggc cgactgatcg gggtcgggtt tgcctcgtat
      541 tgcgagcaga ccgcgcacgg caccagcgtg ttctcggggt ggggcttgcc gatcattccc
      601 ggctacgacc aggcgacggt gcgagtgacg ccggatggag ggctcgaagt ccgcgccggc
      661 gtccactccc atggccaggg catggagacg acgctcgctc agatcgcaca cgaggtcatg
      721 ggcatcgaca tcgccaacat ccgggtcgtg ctcggcgata ccggcaccac cccctactcc
      781 accggcactt atgcctcgcg cagcatcgtg atggcgggcg gcgcggtcgc caatgcgtgc
      841 aagacgctca tcccccgttt cctcgcgatc ggtgcgcatc tcatgcaggc tgccgttgtc
      901 tcgatgaaat acgagaacgg gaaaatcgtc ggaccgcaaa aatcgatctc gctgcgcgag
      961 gtcgccgaag cctggtacct gcggccgcaa gagctgccgc ccgacgtcga cctcggcggg
     1021 ctcgaggcca cggtcggctt tcgcccgaaa cgcgacacgg gcgcgttcag ctatgcctca
     1081 catgccgcgg tcgtggccgt cgataccaag gtgggcgcgg tcgagattct cgactacgtc
     1141 atcgtggagg attgcggcac gctggtgaat ccgatggtgg tcgaaggcca aacgctctga
     1201 ggagcggccc agggcatcgg caccgcgctc tacgaggaga gcccattcgc tccgaatgga
     1261 cagccgcttg cctcgacctt cgccgactat ctcctgccgg gacctaccga gattcctgcg
     1321 attcgcatcc atcatatgga gacgccgtcg ccctacaccg agttcggcat caagggcatg
     1381 ggagaaggcg gtgcgatcgc cccgcctgcg gtaatcttca acgcggtcaa cgatgcattg
     1441 cggccgctgg gcgtggaact gaccgagacg ccgctcaccc cgcgccggtt gctcgaggcg
     1501 atcgaccgcg ccaaggcgtc gagccagccg ccgatggaaa gcgcatgaag gccgcgcgct
     1561 tcgactatgt gcgtcccggt gacgtcgcgg aggcattggc tgccctggcg aaggcagaag
     1621 gcgccaagat tttggcaggt gggcagtcgc tgggaccgat gttgaatttg cggctcgcgc
     1681 gccctgcgct cctggtcgac atctcgcggc ttaaggcgct tcagcaaatg gaagataccg
     1741 gtcgcacgtg gcacatcggt gccggcgtca cccacgctcg tctggaggat gcgcgcggcc
     1801 ggctgtccgg cagcgaaatg ctgtgcgagg tggcggcggc tatcgcctac cgttcggtgc
     1861 gcaaccgcgg caccatcggc ggcagtcttg ctcacgccga cccggcggcg gattggccgc
     1921 ttgcgcttgc cgcgctcggc gcatccgtca atattcgcgg tccgacgccg cgcagcattc
     1981 gggtcgcgca cttcatccaa ggggccttca cgacgacgct cgcggcaaac gagatcatcg
     2041 aatcgatcgt tatcccgaag ctgtcacgcg ctggacggta tggcttctac aaattctgtc
     2101 gtaagacggg cgagtttgcc gaagcgagcg cggccgccgt gttcgatccg gaatccggcg
     2161 tggctaatat ctttctcggt gcgctgcgcg gaacacccca gtcgctcgcg tcattggcgc
     2221 agcaggtggc gaaacacgga gggagcgccg cctcgaccga attcataaac caggcgctcg
     2281 cgcaatcggt tggcgatctc gatgcggtcg agcgcagcat ggcggcagcg gcggtgacgc
     2341 gcgccttgac gcaggtgctc gcgtcgtgac gccggttgcg ctgagcgtga acgggcggaa
     2401 ggtccaggcg ctggtcgagc cgcgcaccca tctggccgat ttcctgcgcg agcattgccg
     2461 tctgacggga actcatctcg gatgcgaaca cggcgtatgc ggcgcatgca ccgtgttgat
     2521 cgacgataag cctgcgcgct cctgcatcac ctatgcggtt cagtgcgacg gctttgcggt
     2581 gcaaacgatc gaagggttcg aggacgatcc gatcatgcag gagctgcgca cggcattttc
     2641 gcgtgagcat ggcctgcaat gcggcttctg tacctccggc atgctgatcg cggcgcgcga
     2701 catcgtgcaa cggctcccgg acgcggacgc gcaccggatc cgggtcgagc tttccggcaa
     2761 tctgtgccgg tgcacgggat atctgggcat catcaacgcg gtggaaagcg tggtcgcggc
     2821 gcgccggtcg aaacccatgg ccgtcacatc cgcggcggct gcgcggccag ctcctcttca
     2881 tgcgttcgtg ccgacgggcg aagtcgcgcc gccgccgatg accgcgccag cgcccgcggt
     2941 cgaagagata cgcacaggtt ggacgcggtt cgaggagagt tttgtggtgg cgatatcgcc
     3001 ggcgacggta tgggcgatgt tcgcggacat cgctgccgtg gccgcttgcc tcgacggggc
     3061 tgagttgacc gagcacgacg cgaataccgc gaagggaaag atgcggataa agctcggtcc
     3121 gatccaggcc ggcttctccg gctccgccgt gatcgaacgc gacgatcggg cgcagcgcgg
     3181 tatcattcgc ggcgcgggca gcgacaaggg taccggctcg cggaccaagg gcgaaatcgt
     3241 ctaccgcctt acgccggagg ccgacgggcg gcagacccgg gtttccgtga cggtcgaata
     3301 tagcctgcaa ggccccctcg cccaattctc gcgctcgggc cttgtcctcg cgcttggccg
     3361 tcgcttggtg tccgattttg ccgccaatct caacgcgcag ctcgcgggag caaaaatacg
     3421 cgcaacgccc ggcgtgccgt tcaatgccgg gcggttcctc tggtcgtggt tcgttgaccg
     3481 gctacgccgc ctcttcggcc gctaagaaag atatgatggt atactgatga ctcgcaaaga
     3541 ccttgccgcg gctgctcaag gttctgcgct gagctattgg ccttgtgaaa aatcaaagct
     3601 atgctgaacg atctcgtttc gggccgcaga cgatggcatg gcaaagggcc gcatccctca
     3661 gtgaaatcga cgtggacggc gtgctgggcg tggacgtgaa cggctcgccc gtcgcgctct
     3721 accggttatc gaacgaggtc tttgccacct ccggaatttg tacccatgcg ctggcgttgt
     3781 tgagcgaggg tttcgtcgag gacggcaaga tcgagtgccc gctgcatcag ggtcaattcg
     3841 acattcgctc gggcagggcg ctgtgtgcgc cggcgacgga ggatctctgc acctacgccg
     3901 tgaagctcga aggcgatgac gtcttcatcg atatggagcg gccggccgcc tcggcgcagg
     3961 tcgtggcgaa cgccgcgccg gatcgcaaag cggggggcgg cgttcgggct gccgaagaag
     4021 acaataaggc gaatactcgc aagattgccg ccatgaacat ggatcctgag ctttcgctga
     4081 cgaaacgtta tgtctggccg gcggaagggc tcacgcgcat ccccgattgg gtctacaccg
     4141 atcagacgat ctatgagcgc gagatcgaga agatttttca tggccgtacc tggaactacg
     4201 tcgccctgga atgcgaagtg cccaaggccg gcgatttcat ccgctccaat gtcggtccaa
     4261 cgccggtggt cgtcgttcgc ggcgacgatg gctccatcaa tgtcttcgag aaccgctgtt
     4321 cacatcgcgc cgcagaattc tgtcgcgagc tcagcggcaa cgtcaaagaa ttcgtctgcc
     4381 cttaccatca gtggacctac gatcttcaag gcaatctcgc cggcgttcca ttccgacgcg
     4441 gcgtaaacgg aaagggcggc atgcccgccg atttcgacag cgcgcagcat ggattgcgtc
     4501 ggctcaatgt cacgactcac cgcggcgtcg tgttcgcttc ctatgtccgg gacatggaat
     4561 cgttacagga ttacctcggg ccggaggtgc tcaaggagtt cgaggcgact ttcgacggtc
     4621 gcaagccgcg gcttctgggc tactaccgcc acacgctgcc cggcaattgg aagctctacc
     4681 acgagaatct aaaggaccct tatcacgcca cgctgctgca cacgttcctg gtgacgttcg
     4741 ggctgttggt cgcgggcaac cgatcgctca tgcttgcgga cgcgaccggg cggcacgggg
     4801 tcatggcgtc ggccaagtcg gagcgtaaga gcatttccag cgatgccaag aaggagatgc
     4861 gcgcctatcg cgacggcatg acgctggcgg agccgcgctt catggacttc atcgaggagt
     4921 tcgacagtcc ctggtcggtg acgatggcaa ccatctggcc gaatctgatc atccagcgcg
     4981 agatgaatac gctcggcgtg cgccaaattg taccgactgg tccgcacgag ttcattatga
     5041 agtggacgat gttcggcttc gaggacgacg acgacgagat gacccgccat cgtctgcgcc
     5101 agggcaatct catgggcccg gcgggcttcc tcggcctgga ggacaacgag gcgatcaagt
     5161 ttgtgcaaga cggaatgcag catgttccgg gcggccagca tctggtgaag ctcgatcccg
     5221 cggttgcgtc cggcacttcc gatagtttga tctccgaagc atcgatccgg gccatgtatc
     5281 agcactggcg cacggagatg gggctctaga accatgggtg aacccgctct gacgcgtgat
     5341 cacccgcccg acgccgggtc gccggattcg cgcctgcaag ctctgctgtt gcatcacgag
     5401 atcgaatcct tcaatgcgcg atacgcgcag gcgctcgacg agcagcggct ggcggattgg
     5461 acggagatgt tcaccgacga tgcgctctac gttgtcctgt cgcgcgaaaa tttcgaccgc
     5521 aggctaccgg tcggcttgat ctattgtgag aacaagcgaa tgatccacga ccgcgcattc
     5581 gcgctcatgg aaacttcgat gtttgcgccg cgttatttgc ggcacatcat cgcaaaccag
     5641 gttgttgtga gtgcagacag caacgcaatg gtgaaggccc gcgcgaacta tgtcgtgctt
     5701 caggtactgt tcgatcgtcc tgatgcgacg ctgcatcagg tcggcgtcta ctatgacgtg
     5761 ttccgtcgcg taggtggcga gctcaagctc gccgagcgcc gttgcgtcta cgacaatctt
     5821 ctcgtgccga atgccctgtg cgttccggtc tagagtcggc ggaccgacag cagcctctct
     5881 taccccggcg gcgatcgacc ggctcgcagg ccggcattcc aggatcgcca caaaaacgaa
     5941 acaccctaaa tgcccaaggt cattccacca gcgcgagcac acgcgccgga ctggcgtctc
     6001 cacctttgat ctttaggggc gctgctatca tcacaaacct gtttttccca atctgggaaa
     6061 gattggtgac gaactgaatc atgggaatgc ccttctcgag cagcgcgagg tggtttgcga
     6121 cccacacttc gcctggatcg agtttgaccc ccctccagat tgccttttcc ggaaagacat
     6181 ccatcgccac cgcggacacg tttttcttta ccatcagctc acccacgccg ggctcaagat
     6241 agggcatctg ctcccagaac tcaggcttgc cccacgtctt ctccgtccaa ccggtattga
     6301 tgacaggaat gacatccgga ccgaagtcca ccccagtatc cagaatgtcc ttcgccgaca
     6361 ccgac
//
LOCUS       DATLJT010001279         3583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2398386,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001279 DATLJT010000000
VERSION     DATLJT010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398386"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..721
                     /locus_tag="VM910_29405"
     CDS             74..721
                     /locus_tag="VM910_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI66662.1"
                     /translation="MPEVIFTGPAGRLEGRYHPAKQKNAPIAMILHPHPQFHGTMNHQ
                     IVYQCYYAFAHRGFSVLRFNFRGVGRSQGSFDHGTGELSDAASALDWAQTINPEARAC
                     WVAGFSFGAWIGMQLLMRRPEVEGFISIAPPANLYDFSFLAPCPSSGLIVHGEKDAVV
                     PPKDVNTLVEKLKTQKGIVIDQQIIPGANHFFDGKLEPLMETITAYLDMRLANVR"
     gene            complement(718..1821)
                     /locus_tag="VM910_29410"
     CDS             complement(718..1821)
                     /locus_tag="VM910_29410"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HVI66663.1"
                     /translation="MMLTALGLMSGTSLDGVDVALIETDGRRISALGPSGYRPYTEAE
                     RILLRQALAEAVHLPRRDARPGALAEAERVVTVAHAEAVAAFKTQHRITPEDIDIVGF
                     HGQTVLHRPEKRLTVQIGDGVALAKAIHIPVMHDFRAADVAAGGQGAPFVPVYHRALA
                     QSLERDGPIVVVNIGGVSNITYIDGHETLIACDTGPGNALLDDFMFRIMHQPFDTEGR
                     LAAQGRADQAWIARALQLPFFKLPPPKSLDRNDFASLRLGEMKAEDGAATLTAFTAAA
                     IARVIPLLPKVPKNWIISGGGARNLTMMRMLRERLAPARVDAADALGWATDAIEAQAF
                     GFLAARGLKGLPLSYPATTGVPIPMTGGVIARP"
     gene            1980..3233
                     /gene="tyrS"
                     /locus_tag="VM910_29415"
     CDS             1980..3233
                     /gene="tyrS"
                     /locus_tag="VM910_29415"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVI66664.1"
                     /translation="MSVFKSDFLNVLQERGFIHQCSDFAGLDALAAKRQATAYVGYDC
                     TAPSLHIGNFLTMMMLYWLQQSGNKPITLMGGGTTMVGDPSGKDETRALRSIDEIEAN
                     KASIRGVFAKVLNFGDGPADAIMLDNAEWLTKLNWIEMLRDVGRHFSVNRMLTMDSVR
                     LRLEREQEMSFIEFNYMVCQAYDFVELSRRTGCRLQMGGSDQWGNIVMGVDLGRRMGT
                     PQLFALTTPLLTTASGDKMGKTAKGAVWLNADQFSPYDFWQYWRNTEDADVVKFLKLF
                     TTLPMREINKLAALKDAEINEAKKALADAATTLLHGKEAAREAAETARQTFEEGAIAQ
                     SLPTVEIPRGELEAGSGVLTLFVKAGLVASNGEARRQIKGGGLRVNDAAVTDEKMMLS
                     NADLTPEGVIKLSLGKKRHVLLRPA"
     gene            3291..>3583
                     /locus_tag="VM910_29420"
     CDS             3291..>3583
                     /locus_tag="VM910_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66665.1"
                     /translation="MDKNSPLEETLAPTRPAHVALVVLGLCFALSVLGRGLAESFTVF
                     LKPISESFGWDRAEVVSVYSLTALAGGMSAPVIGRLFDHSGPRAVYCLGLLLLG"
BASE COUNT          691 a         1130 c         1104 g          658 t
ORIGIN      
        1 gcgcgccgat gaagtctcca acaatcggtt cattgccgag acagcaaccg agaggtcgat
       61 ccggggatcg ctaatgcctg aagtcatttt caccggcccg gctggccgcc tcgagggccg
      121 ttatcatccg gccaagcaga agaacgcgcc gattgcgatg atcctgcatc cgcatccgca
      181 attccacggc acgatgaacc accagatcgt ttaccagtgc tattacgcat tcgctcatcg
      241 cggtttttcg gtgctgcgct tcaatttccg cggcgtcggc cgcagccagg gttcgttcga
      301 tcacggcacc ggcgaattgt cggatgcggc gtccgcgctc gactgggcgc agactatcaa
      361 tcccgaagcg cgcgcatgct gggtagcagg cttctccttc ggcgcctgga tcggcatgca
      421 gcttctgatg cgtcggccgg aggtcgaggg tttcatctcg atagcgccgc ccgccaatct
      481 ctacgacttc tcctttctgg cgccctgccc ctcctccggg ttgatcgtgc acggtgagaa
      541 ggacgccgtg gtaccaccga aggacgtcaa cacgctggtc gagaaattga agacacagaa
      601 gggcatcgtg atcgaccaac agatcatccc cggtgccaat catttctttg acggcaagct
      661 cgagccgctg atggaaacga tcacggccta tctcgacatg cggctcgcca acgtccgcta
      721 aggccgcgca atcaccccgc ccgtcatcgg aatcgggacg ccagtggtgg cgggatagct
      781 gagcggcagg cctttcaagc cccgtgccgc caaaaagccg aacgcctggg cctcgatcgc
      841 gtccgtcgcc cagccgagcg cgtcagccgc atcgactcgc gcaggcgcaa gccgctcgcg
      901 cagcatccgc atcatggtga ggttgcgcgc cccgccgccc gagatgatcc agttcttcgg
      961 cactttcggc agcaagggaa tgacccgcgc gatcgcagcc gccgtaaagg cggtgagcgt
     1021 cgccgcgcca tcctcggcct tcatttcacc gagccggagg gaggcgaaat cgttgcggtc
     1081 gagcgatttc ggcggcggca gcttgaagaa gggcagttgc agcgcgcgag cgatccacgc
     1141 ctggtcggca cgaccctgtg ccgcaagccg cccttcggtg tcaaagggct ggtgcataat
     1201 gcggaacatg aagtcatcga gcagcgcatt gccagggccg gtgtcgcagg cgatcagcgt
     1261 ctcgtggccg tcgatgtagg tgatgttcga aacaccgccg atattgacca cgacgatggg
     1321 tccatcgcgc tccagcgact gcgcgagcgc gcgatgatag accggcacaa acggcgcgcc
     1381 ctgcccgccg gcggcaacat cggcggcgcg gaagtcatgc atcacaggga tgtggatcgc
     1441 ctttgccagc gcgacgccgt cgccgatctg cacggttagc cgtttctccg gccggtgcag
     1501 cacggtctgg ccatgaaagc cgacgatatc aatgtcctcg ggggtgatac ggtgttgcgt
     1561 cttgaaggcg gcaactgctt ccgcatgcgc tacggtcacg acacgctcgg cctccgcaag
     1621 cgcgccaggc cgggcgtcgc gccgcggcaa atggacggct tcggccagcg cctgccgcag
     1681 caggatgcgc tccgcttccg tgtaggggcg ataacccgac ggcccgaggg cgctgatccg
     1741 ccgaccgtcg gtttcgatca aagcgacatc caccccgtcg agcgaggtgc cgctcatcag
     1801 gccgagtgct gtcaacatca tcgttggagc ctcgcgagtc ctaccccgga cgcccagctt
     1861 gtgccaaagg gatacatctt atattgccac agcgcgccga tctgcggcag cccaactcag
     1921 ggggttcccg caatttgctg caaataccgt aaaacaagaa tgatcaaggt cgcagaaaga
     1981 tgagcgtttt taagtccgat ttcctcaacg tgctgcagga acgcggcttt atccaccaat
     2041 gctcggattt cgcgggcctc gatgcgctcg ctgccaagcg acaggcgacc gcctatgtcg
     2101 gctatgactg caccgcgccc tcgctgcata tcggcaattt tctcaccatg atgatgctgt
     2161 actggctcca gcagagcggc aacaaaccga taaccctgat gggcggcggc accaccatgg
     2221 tgggcgaccc ctccggcaag gacgagacac gcgccctgcg ctccatcgac gagatcgagg
     2281 cgaacaaggc gagcatccgc ggcgtgtttg ccaaggtgtt gaattttggc gatggccccg
     2341 ccgatgcaat catgctcgat aacgcggaat ggctcacaaa actcaactgg atcgaaatgt
     2401 tgcgcgacgt cggccggcat ttctcggtga accgcatgct gaccatggac tcggtgcggc
     2461 tccggctcga gcgcgagcag gagatgagct tcatcgagtt caactacatg gtttgccagg
     2521 cctacgattt cgtcgaactg tcgcggcgca ccggttgccg gctgcagatg ggcgggtcgg
     2581 accagtgggg caacatcgtg atgggcgtcg atctgggccg ccgcatgggc acaccgcaac
     2641 tcttcgcgct gacaacgccg ctgctcacga cggcatcggg cgacaagatg ggcaagacgg
     2701 ccaaaggtgc cgtttggctg aatgcagatc agttttcgcc ttacgatttc tggcaatact
     2761 ggcgcaacac cgaggacgcc gacgtcgtca aattcctgaa gctcttcacg acgctgccca
     2821 tgcgcgagat caacaagctc gccgcgctca aggacgcgga aatcaatgag gccaagaaag
     2881 cgttggctga cgcagcaacc acgctgctgc acggcaagga ggccgcgcgt gaggcagcgg
     2941 aaaccgcgcg gcagacgttt gaggaaggcg caatcgcgca aagccttccc acagtcgaaa
     3001 ttccgcgcgg cgaacttgag gccggaagcg gtgtcctgac gctgttcgtc aaggccggcc
     3061 tcgttgcctc gaatggcgag gcccgccgtc aaatcaaggg cggcggcctg cgtgtcaatg
     3121 atgcggctgt caccgacgag aagatgatgc tgtccaacgc cgatcttaca ccggagggcg
     3181 tgatcaaatt gtcgctcggc aagaagcgcc acgtcctgct caggcccgca taggcacatt
     3241 cgcttttggc gcttgcggcg aacgcggaaa cacgctccca cttcctgccg atggacaaga
     3301 actcgccgct ggaggaaacg cttgcgccga cacggcctgc gcacgtcgcc cttgtcgtgc
     3361 tcgggctttg ctttgcactg tctgtgctgg gacgaggcct ggcagaaagc ttcaccgtct
     3421 ttctcaaacc gatttccgaa agctttggct gggaccgtgc cgaagtggtc tcggtctatt
     3481 cgctgacggc cttggccggt ggaatgtccg cgccggtaat cggccggctg ttcgatcatt
     3541 cagggccgcg cgcggtctat tgcctcgggc tgctccttct tgg
//
LOCUS       DATLJT010001280         4544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3317578,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001280 DATLJT010000000
VERSION     DATLJT010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3317578"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1370
                     /locus_tag="VM910_29425"
     CDS             387..1370
                     /locus_tag="VM910_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66666.1"
                     /translation="MKRREFLALVGGTAAAWPLSVRAQQPAMPVIGYLSARSPEDTAH
                     LVEAFRRGLGETGFFEGHNVTIEYRWALGHQDRLPALAADLVQKPVTVLVSTGGESAA
                     LAAKAATSTIPIAFIIGGDPVNLGLAASYRRPGGNATGISILTATLEPKRLELLRELM
                     PHVSTIGALLDPNFPPYEGQLRDLREAARALDLQVQELRASTEAEIDLAFETVVRQRI
                     AALTVVAGPFFDTRREKLVALAAHHALPTMYHFREFPVAGGLISYGIDSRVSYRHIGV
                     YAGGILKGEKPAELPVLQPTKFELVINLKTAKTLGLKVPLTLQVAADEVIE"
     gene            1420..2049
                     /locus_tag="VM910_29430"
     CDS             1420..2049
                     /locus_tag="VM910_29430"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVI66667.1"
                     /translation="MDAAFVGESRHAADIAAVADASRIRLLLLGRGDSSRQQNIRLST
                     NGEIMRKLILLVLFLAAVPLTARGDSKEQLAGAWSLVSIEFVRPDGSRSSTFGANPRG
                     TAFFDASGHYIISVMRSDRPKYASNNFAEGTAEENKATAQGTITYFGTYSVSEPDRTI
                     AIHIEGSSFPNWDGANQKRLFVFSGDELKLISPAASGAGTAEVVWRRAK"
     gene            complement(2423..2617)
                     /locus_tag="VM910_29435"
                     /pseudo
     CDS             complement(2423..2617)
                     /locus_tag="VM910_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084757.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2880..3359)
                     /locus_tag="VM910_29440"
     CDS             complement(2880..3359)
                     /locus_tag="VM910_29440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66668.1"
                     /translation="MPNTYLTPEDEQYYGRDLIDFTTRAATQAVAPYLQNLEQQNATL
                     QRRLAREQRHRLDQQVTAAVPNWPEIDQNPRWHAWLSTVDPLSGCPRQRLLDDAIASG
                     SAERCAAFFQSFMREQRDSRSTPAHSRPRPAQSSSNAAANQSELRNEAKRCVERQGG"
     gene            complement(3389..3613)
                     /locus_tag="VM910_29445"
     CDS             complement(3389..3613)
                     /locus_tag="VM910_29445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66669.1"
                     /translation="MHTSKVGERQQGAKFARGGRDHMFPKQGAGAAPAGRAAKRQPAP
                     VTPRAAKGGGKSNVGGVSRPAVGGRTSPPR"
     gene            3695..4144
                     /locus_tag="VM910_29450"
     CDS             3695..4144
                     /locus_tag="VM910_29450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66670.1"
                     /translation="MKTLPDGRATYTPRNSPDPYIDACLLADAIASADAVMFNADGKP
                     MWLKGETLVLIGRDTLQQFVAEYVVTPKAVFTDSGELAVRFEPYAINEVEIRLLLGGK
                     SPQEGGLKFRLPLSQMPPSPVVQPEPPVRAGAEDAAARVSHSALAAR"
BASE COUNT          844 a         1371 c         1367 g          962 t
ORIGIN      
        1 gtatctcgtg aggtcaggga tgcgtgtgct ggtgtcgatc tggggtttgc gccagcgggc
       61 tccgaaacgc tgaagggatt ttcagaatcg gttcagctct tttccccggc tcattgatat
      121 cgcttcctga acagcgcgag aacgtcgtcg gcgcgaatgt ccgaatgtgg cacgaaccgc
      181 ccgaacgaag atgtccgcat tcacggcagc tttcggaggg ataacggaca tgccacggac
      241 attgcgcggc ggcagaatct aaaccctgag cggacattca caacgcggaa caattagtcg
      301 cagttcgggt ccctctcgcg ccgttccaac agccgtaccg ctgtggcacg atacggcgcg
      361 tagttttgat attcctgggg gcatttgtga aacgccggga attcctcgct ttggtcggcg
      421 gcacggcagc tgcatggccg ctctcggtgc gcgcgcagca gccggcgatg ccggtgatcg
      481 gatatctcag cgctaggtcc cccgaagata ccgcgcactt ggtggaggca tttcgacgag
      541 gcctgggcga aacgggcttc ttcgagggcc acaacgtgac gattgaatac cgctgggcgc
      601 tcggtcacca ggatcgactt ccggcactcg ccgcggacct cgtacaaaag ccggtgaccg
      661 ttctcgtttc cacgggtgga gaatcggcgg cgctggcagc caaggctgcg acctcgacca
      721 ttccgattgc attcatcatc ggcggcgatc cggttaatct ggggctcgca gcgagctaca
      781 ggcggccggg cggaaatgcg accggaataa gtatcttgac cgccactttg gaacccaagc
      841 ggctcgaact gttgcgcgag ctcatgccgc acgtctcaac tatcggagcc cttttagacc
      901 cgaacttccc gccctatgaa ggtcagctac gcgatctgcg tgaggcagcg cgcgccctcg
      961 acttgcaggt tcaagaattg cgagccagca cagaggcaga gatcgacctg gcattcgaaa
     1021 ctgttgtacg gcagcgcatc gccgcgctca cggtcgtcgc cggaccgttt tttgacacac
     1081 gacgtgaaaa acttgtggcc ttggcagccc accatgcgct gccgacgatg taccatttcc
     1141 gcgaatttcc tgtggctggg ggactaatta gctacgggat cgattctcgc gtcagctatc
     1201 gtcatatcgg agtttacgcg ggcgggattc tcaagggtga aaagccggcc gaattgccag
     1261 tcctgcagcc aaccaagttc gagctggtca tcaatctgaa gacagcaaag acgcttggac
     1321 tgaaagttcc actcaccttg caagttgccg ccgacgaggt gatcgaatag agcagccaat
     1381 ggccgcctgt ggcgcttccg cgacatcgta agatcgcaga tggacgccgc ttttgtgggg
     1441 gagagccgtc atgcagcgga catcgccgct gtggccgatg cgagcaggat acgtttgctc
     1501 ttgcttggcc gcggtgattc ttcgcgtcaa cagaacatcc gcttgtcaac gaacggggaa
     1561 atcatgagaa aactcatact attggtccta ttccttgcag cggtgccgct gaccgcgagg
     1621 ggggattcga aagagcaact cgcaggggct tggtccctcg tctcgattga attcgtgcgc
     1681 cccgacggca gcagatcgtc aacattcggt gcgaatccta ggggtaccgc gttttttgat
     1741 gccagtggtc actacatcat ttcggtcatg cgctcggacc gccccaaata cgcaagcaac
     1801 aacttcgcgg aaggtacagc cgaagagaat aaggcaacgg cgcaagggac tattacctac
     1861 tttgggacat attcggtaag cgagccggac cgcactattg cgattcatat tgagggtagt
     1921 tcgttcccaa attgggatgg tgccaatcag aaacgacttt ttgtgttttc gggagacgag
     1981 ctcaaattaa ttagccctgc cgcgagcgga gcagggaccg ccgaggtggt ttggaggcga
     2041 gcaaagtaag tcacatcaac gctggtcgcg gcgtctccaa cggagccgct gtatggccgt
     2101 tattggcact tctcggacct cgcgagatgt ccgacttgag tccgcaatgt gggcaaaagc
     2161 ggacgttgat caggccgcgc tcatctagcg gcgcggcggc tacctcgtcg agaaattgct
     2221 tgaacaagtc acgcagctca agcggcgccc cctcgctgcc gtccgacagc aacggcacgg
     2281 cggaataggc cgcacgatcg acgcgccagc cggtgatcgt cggcgcgggt tcgccccgcg
     2341 gcccgatcaa gccgcgctcg cctctcgagc cagccgtggc ccgggaattc ctgtactcac
     2401 tacctatctg cttgagaaaa aaaagcccga tcacaaactg tgtcgcaatt ccaggccggc
     2461 caccctcgct gtacagcggc gcgatctcgc cgtcgatgaa ctcccagtcg atcttgcccg
     2521 cgagctgcac tagctcgtgc ctcatgttga tgatctggtc cagccgcgcg cggaacagat
     2581 cacccgatgc tgtcgtctcg tgcttcttcg gccgcatcgc tccctccggt gcggcgaccg
     2641 aatcatgctt ctcgattcga gggaatcgcg aaaacgaatt tgcaagcttc caggccctca
     2701 gacacggaaa ccttgcaatc tcaagtcaac aaagccgcca aaacgactct cgctcagtgg
     2761 gttagaaatt cttcacgggc gactaattca gtttgtgacg ttacttcgta aggtagatcg
     2821 gggccaggat ccgtttctcc ttctgggccg cgaagatgtc ttgctccagg cgggcgaact
     2881 catcctcctt gccgttccac gcaccttttc gcttcatttc gtagttcgct ttgatttgct
     2941 gcggcgttgc tgctgctctg tgctggtcgg ggacggctgt gtgccggagt gctcctgctg
     3001 tccctttgct ctcgcatgaa gctctgaaaa aatgcggcgc aacgctcggc gctgccggat
     3061 gcgatcgcgt cgtcgagcag gcgctggcgc ggacatccgg acagcgggtc aactgtcgaa
     3121 agccacgcat gccagcgagg attctggtca atttccggcc agtttggaac agctgccgtg
     3181 acctgctgat ccaatcggtg ccgctgctcg cgtgccagcc gccgctgtag cgtggcattc
     3241 tgctgctcaa ggttttgcaa atatggcgcc actgcttgcg tcgcggcgcg cgtagtgaag
     3301 tctatgagat cccgaccata gtactgttcg tcttccgggg ttaagtaggt gtttggcatc
     3361 ggggacgctt ccttgcgcct gccgaaaatc accgcggcgg cgacgtgcga ccacccactg
     3421 ccggtcgtga gaccccgccg acattgctct taccgccgcc tttagctgcg cgcggggtga
     3481 ccggcgcggg ctgccgcttg gcggcgcgac ccgccggtgc agctccggca ccctgcttgg
     3541 ggaacatgtg atcacggccc cctcttgcga acttggcccc ctgctggcgt tctccgacct
     3601 tgctggtgtg catgggggtg atccttcttg ccatcgaggg cacaatagct agtgtagtgg
     3661 caatctttgt actacaattc agcgggtcag cagcatgaaa acgctcccgg acggccgcgc
     3721 aacctacacc ccgcgcaaca gcccggaccc ttatatcgac gcctgtctgc tggccgacgc
     3781 catcgcctca gcggacgccg tgatgtttaa tgccgacggg aaaccgatgt ggctcaaggg
     3841 tgaaacgctg gtgcttatag gccgggacac cttgcaacag ttcgtggcgg agtacgtcgt
     3901 cactccgaaa gcggtcttca ccgacagcgg cgagctggcg gttcggttcg aaccctatgc
     3961 gatcaacgaa gtggagatcc gccttttact cggcggcaag agcccgcagg agggcggact
     4021 gaaattccgc ctgccgttgt cgcaaatgcc gccgtcgcca gtggtgcagc ccgagccgcc
     4081 ggtgcgtgcg ggagcggagg acgccgcggc ccgcgtttcg cattcagctt tggcggccag
     4141 atagcctgct gcatccaggt tctcggcttc accggctctg gcgttccggc gcgcgcgcgt
     4201 cgcgaggtcg attctcagcg ggacggttcc tggacgttat cctcctggct ccaatgtgcc
     4261 cgagcttccc gggggtgggc tacgggcctg gctgccgaaa ggtcgtcgcg ccttgtcctt
     4321 gtcggctttc cacgccgtga acagcttgca tgccctgtcg aggtccgcct cggtcgcttg
     4381 cctgcgcgta tccggatgca cgaccttggc gattgcgctc attgtcgcga agctcatcgc
     4441 gcttttgcct ccctgtgtga actccctcgt ttagcggagt tcggccgcaa gattgcgcac
     4501 cctggttttg agcgccttga tctcgcgatc gcgcgccttc ggcc
//
LOCUS       DATLJT010001281         1643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4236265,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001281 DATLJT010000000
VERSION     DATLJT010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4236265"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VM910_29455"
     CDS             <1..715
                     /locus_tag="VM910_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVI66671.1"
                     /translation="HEYMPLRFTQTEPGRVYRKISYGPLLDVFMLDMRSYRGPNGEGQ
                     EESYGPAAYFLGPAQVAWLKRELLNSQATWKVIAADMPIGLIVVYDTDRKWGVEAIAQ
                     GDGPPRGREFEIVDILSFIKRAPVRNTLWLTADVHYTAAHYYDPNKAVFQDFEPFWEF
                     VSGPIHAGSFPQNQLDNTFGPQLVYVKAPSAPNQPPSEGLQFFGHVAIDGATQVMTVT
                     LKDRDDRALWSTTLEPKLG"
     gene            complement(737..>1643)
                     /locus_tag="VM910_29460"
     CDS             complement(737..>1643)
                     /locus_tag="VM910_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HVI66672.1"
                     /translation="FIGNRKAAKDMLPHLLAWQKQHGYPLQFACEATLNIAKQPDILK
                     LMRECSFLGIFVGIETPEADALKAMRKSQNVAVPMMESIKTLNDYGLEVTSGIILGLD
                     TDSDDTEVRLKDFIDISRIPMLTINLLQALPKTPLWDRLKLAGRIADDPALESNVRFL
                     RPYEEVVSMWRRCIAHANDPERLFARFHHQVDATYVNRLIPPARGKLNWANLSGALVL
                     AFRVFLHIGILSDYRRPFWRAARHALRRGQIDAVLGMGFIAHHLIQFTREALRGEQNA
                     SYYSTKTRTAEPMHSPQLAAVRKSA"
BASE COUNT          308 a          517 c          531 g          287 t
ORIGIN      
        1 ccatgaatac atgccgttgc gcttcaccca gaccgagccg gggcgcgtct atcgcaagat
       61 ctcctatgga ccgctgctcg acgtgttcat gctcgacatg cgcagctatc gcgggcccaa
      121 cggcgaaggc caagaggaga gttatgggcc ggcggcttat ttcctcggtc cggcgcaagt
      181 ggcctggctc aagcgcgaac tcttgaactc gcaggcgacg tggaaggtga tcgcggccga
      241 catgccgatc ggcctcatcg tcgtttacga caccgatcgc aaatggggcg tcgaggcgat
      301 cgcccagggc gacggcccgc cgcgcggccg cgagttcgag atcgtcgaca tcctctcatt
      361 catcaagcgg gctcccgtgc gcaacacgct gtggctcacc gccgacgtgc actacaccgc
      421 cgcgcattac tacgatccca acaaggcggt gttccaggat ttcgagccgt tctgggaatt
      481 cgtgtcgggc ccgatccacg ccggaagctt cccgcaaaac cagctcgaca acacgttcgg
      541 accgcagctc gtctacgtga aggcgccgag cgcaccgaac cagccgccga gcgagggcct
      601 gcaatttttc ggtcacgtcg caatcgacgg agcaacgcaa gtcatgaccg tgacgctgaa
      661 ggaccgggac gatcgcgcgt tgtggtcgac cacgctcgag ccgaagctgg ggtgacggca
      721 ccctacaacg aagcgcctac gccgacttgc gcaccgccgc caactgcggc gaatgcatgg
      781 gctcggccgt gcgcgtcttg gtcgagtagt acgacgcgtt ctgctcgccg cgcagcgcct
      841 cgcgggtgaa ctgaatgagg tgatgggcga tgaagcccat gccgagaacg gcgtcgatct
      901 gcccgcgccg caacgcgtga cgcgccgccc gccagaacgg cctgcggtaa tccgacagga
      961 ttccgatgtg caggaagacg cggaaggcga gcaccaatgc gcccgacagg ttcgcccagt
     1021 tgagcttgcc gcgcgcgggc gggatcaggc gattgacgta ggtcgcgtcg acctgatggt
     1081 ggaagcgcgc gaacagccgc tccggatcgt tggcatgggc gatgcagcgg cgccacatcg
     1141 acaccacctc ctcgtacggc cgcaggaagc gcacgttgct ttcgagcgcc ggatcgtccg
     1201 cgatccgtcc agccagcttg agtcgatccc acagcggcgt cttcggcaac gcctgcagca
     1261 ggttgatggt cagcatcggg atgcgtgaga tgtcgatgaa atccttgaga cgcacctcgg
     1321 tatcgtcgga atcggtatcg agcccgagga tgatgccgga cgtgacctcc aagccatagt
     1381 cgttgagcgt cttgatcgat tccatcatcg gcacggcgac gttctggctc ttgcgcatcg
     1441 ccttgagcgc atcggcctcg ggcgtctcga tgccgacgaa tatgccgagg aacgagcact
     1501 cccgcatgag cttgaggatg tccggctgct tggcgatgtt gagcgtcgcc tcgcaggcga
     1561 actgcagggg atagccgtgc tgtttctgcc aggcgaggag atgcggcagc atgtccttgg
     1621 cggctttgcg attgccgatg aaa
//
LOCUS       DATLJT010001282         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1481139,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001282 DATLJT010000000
VERSION     DATLJT010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481139"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VM910_29465"
     CDS             <1..957
                     /locus_tag="VM910_29465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66673.1"
                     /translation="CMLIAVGVGLANERAKLLDAMLRLRKVEAAAAADRAIVETIKKN
                     ARLTELLAQATGSASQREIDEFWRKLVESNDVVAKLGRQGVSLVALKEEGDYFAKMQR
                     LLKEGIDLDKIARGLALASAIDEAGFANAMPKLTPEQVTALLEKGIAASKPRSENDRG
                     NGHNWPPIINLSEAGGYYFATGSAVLTPDFATALRTVVISRLLQIAESFDVDVIEVIG
                     HTDEQPVTNRVSNLDRHLSSVTLGGADAGVLQWADNAGLGLARALAVVKVLASDARLG
                     AFRILPLSGAQLIDTDGRLTRWDEQGDVRERRRIEIRMRKSS"
     gene            complement(1002..1973)
                     /locus_tag="VM910_29470"
     CDS             complement(1002..1973)
                     /locus_tag="VM910_29470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66674.1"
                     /translation="MMALATTNPAILRRWAISAAVVVLVHSAIVAAVVTWRKATVPAE
                     PPGPIVIELAPMPAAPTTQQAALPPAPEVPSTISPDKRPEKVEQKAEEKTAARGEEKA
                     ERSQFEESPPVTAPVILAPPESAEEGNRADTKGVPGGEAGGAAQAGGGSNPIDARIGE
                     QSRQGFKKAAKANDWTRAIMGRPSKNFAGRQQPRGPGTASGMARNAIGMLVQDPAGAM
                     GTKASTAANATKNAVGRTAMNTVGGAAAVNGTAANAIGVSKPIRTRAPRTNIGQRGIA
                     PLASSATSPNASINGTSMIRPASATAVLGGPAKNAAGAINGTAMRPR"
     gene            complement(1970..>2103)
                     /locus_tag="VM910_29475"
     CDS             complement(1970..>2103)
                     /locus_tag="VM910_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVI66675.1"
                     /translation="DRPIYLRADKAVPYGELMEVMNLLRAAGYLKVALVGLEKASAS"
BASE COUNT          369 a          683 c          656 g          395 t
ORIGIN      
        1 tgcatgctca tcgccgtggg cgtcgggctc gccaatgaac gcgccaaatt gctcgacgca
       61 atgctgcgcc tgaggaaagt cgaggcggcg gccgctgccg acagggcgat cgtcgagacc
      121 atcaagaaaa atgcgcggct gacagagctc ctggcgcagg cgaccggttc ggcgtctcaa
      181 cgcgaaatcg acgaattctg gcgcaagctg gtcgagagca acgatgttgt tgccaagctc
      241 gggcggcaag gcgtctcgct tgtcgcgctc aaggaagagg gcgactattt tgccaaaatg
      301 cagcggctgc tgaaggaagg aatcgatctc gacaagatcg caagagggct agcactcgcg
      361 tctgcgatcg acgaggccgg ctttgccaac gccatgccga agctgacgcc ggagcaggtt
      421 accgccctgc tcgagaaagg gatcgcggcg tccaagccgc ggagcgagaa cgatcgtggg
      481 aatgggcaca actggccgcc gatcatcaat ctgagcgagg ccggcggata ttacttcgcg
      541 accggcagcg ccgttttgac gccggacttt gcaacggccc tgcgcacggt ggtgatcagt
      601 cggttgctgc agatcgcgga atcgttcgac gtcgacgtca tcgaagtcat cggacatacc
      661 gacgagcagc ccgtcaccaa tcgtgtttcg aatctggatc gccacctgtc gtcggtgacg
      721 ctgggaggtg ccgacgcggg ggtcctgcaa tgggccgaca atgccggcct cggactcgcg
      781 cgcgcgctgg cggtggtcaa ggtgttggcc tccgacgcgc ggctcggggc ctttcgcatc
      841 ttgccgctat cgggcgccca actcattgat accgacggca ggctcacccg ctgggacgaa
      901 cagggcgatg tccgtgagcg tcgacgaatc gaaatccgaa tgcgaaagtc cagttgagca
      961 gcggcgggcc ccctcccagg gcgccatagc tcccatcata gctatctggg ccgcatcgca
     1021 gtcccgttga tcgcacctgc agcattcttt gcgggaccac ccagcacagc ggtagcagaa
     1081 gccggacgga tcatgctcgt gccgttgata cttgcattcg gcgacgtggc actggacgcg
     1141 agtggagcga taccgcgctg accgatattc gtccgcgggg cgcgcgtgcg aatgggcttg
     1201 ctgacgccga tggcattcgc ggcggtgcca ttgacagccg cagcgccacc aaccgtgttc
     1261 atcgccgtac gaccgacggc gttcttcgtc gcattcgcgg cggtcgaggc cttggtaccc
     1321 atggcacctg ccggatcttg cacgagcatc ccaatggcgt tgcgggccat accacttgcc
     1381 gtgcctggac cgcgcggctg ctgccgtccg gcaaaattct tcgatggacg gcccataatg
     1441 gccctcgtcc aatcgttcgc tttcgcagcc ttcttgaagc cctgacggga ttgctcgcca
     1501 atgcgggcat caattggatt gctgcctcct ccggcctgcg cggctccacc ggcctcaccg
     1561 cccggcactc ctttcgtgtc cgctctattc ccctcttcgg cgctctccgg cggcgccaaa
     1621 atcacaggtg ccgtcacggg cggagattcc tcgaattggc ttcgctccgc tttttcctcc
     1681 cctctcgccg cagtcttctc ctcggccttt tgctcgactt tctccggtct tttgtcgggc
     1741 gagatggtgg acgggacctc gggcgccggc ggaagcgcgg cctgctgcgt tgtcggcgcg
     1801 gccggcatcg gcgcaagttc gatcacgatg gggccaggag gctcagccgg cactgtcgct
     1861 ttgcgccaag tcaccaccgc cgccacgatg gcgctatgta caagcaccac caccgcggcg
     1921 ctgatcgccc agcggcgcag aatggcgggg ttggtcgtgg ccaatgccat catgacgcag
     1981 acgctttctc caaccccacc agcgccacct tgaggtagcc cgccgcgcgc aagagattca
     2041 tgacctccat caattcacca tatggcacgg ccttatccgc gcgcaagtag atgggcctat
     2101 cct
//
LOCUS       DATLJT010001283         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2860284,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001283 DATLJT010000000
VERSION     DATLJT010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860284"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VM910_29480"
     CDS             <1..224
                     /locus_tag="VM910_29480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66676.1"
                     /translation="GPPGPAFGIRIVRSNCDATNCSVQCSEDELLLTAYCGARRNAAV
                     IPSERAATCRSPVPANSPLVAACVKIPAQ"
     gene            392..1381
                     /locus_tag="VM910_29485"
     CDS             392..1381
                     /locus_tag="VM910_29485"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVI66677.1"
                     /translation="MGFLPEKELSDKCRGVCFAAVMAVLNVTALLPAGAETVATFTRN
                     PSNPILRGVRIGGEIAAKSLDAEIVHFIPRSEAPAEQLGLVDEVVRIKPDAIVFAPFD
                     PKAMVPAVDKLNAAGIPVTNVNERLAGGNVVAYVGTDDYQLALTTARYLINAMGKKGN
                     VVILEGPDNLPTSIARVKAYKDALKEFPDVKLLASKSANYARAPAIEVMKSFLRLYPQ
                     IDGVLAANDPMAIGAVEALKAANRKAPVVGINASKEVIDFVKSGDILGSGDYNGFVQG
                     CLGTQIAIHNLRKQPTPKEVNLKAVVMDKTNYQDYETPIERRSCPTLESVSAN"
     gene            complement(1445..1984)
                     /locus_tag="VM910_29490"
     CDS             complement(1445..1984)
                     /locus_tag="VM910_29490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66678.1"
                     /translation="MSFYRAFPVFSTAFAVLYLLAATFNYALFTYHPLLHELDLLAQP
                     AKAGPAMYWYGWITTAAIGALAVAAVATFVTERWERWIQWTITFACTYAVFYVIANGV
                     AVFIYDRSSYELEFLKHWATPATGALVATVIASFFFPIRWTQRVWSGWTGVIPLSVML
                     VFVYLLRGWFIPSVGLILK"
     gene            complement(1981..>2182)
                     /locus_tag="VM910_29495"
     CDS             complement(1981..>2182)
                     /locus_tag="VM910_29495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66679.1"
                     /translation="VGIFDLKEHFSTVALGLLPAYWYYWRQPLSAERASIRKFLTTII
                     AFTIWWNFLVGHFTNNIRGLWS"
BASE COUNT          499 a          667 c          635 g          381 t
ORIGIN      
        1 ccggtccgcc cggccccgca ttcggcattc ggatcgttcg atcgaattgc gacgccacga
       61 actgcagcgt gcaatgcagt gaggacgagc tgctcttgac cgcatattgc ggagcccgac
      121 gcaacgccgc cgtcattccc agcgagcggg cggcaacctg ccgcagtccg gtgccggcca
      181 atagtccgct ggtcgcggca tgcgtgaaga taccggcgca atagtctgcc cgcgcggcga
      241 cggcggtgcg agcaccgcgg gacgctagga ctgcgatgca tacgttcggg tcaatccggg
      301 ctggaggcca tcgccgaacg tttgctagag tcgcggcgac gttcggtctc ggcgcgatca
      361 aattccatac gacgcgtgca tggaggacat catgggcttt ctgcccgaga aggaactctc
      421 ggataaatgt cgtggtgtct gttttgcagc cgtaatggcg gtcctgaacg tgactgcgct
      481 cttgccggct ggcgctgaga ccgtcgcgac cttcacgcgc aatccgtcca atcccattct
      541 tcgcggcgtg cgcatcggcg gcgagatcgc ggcgaagtcg ctcgacgccg agatcgttca
      601 tttcatcccg agaagcgagg cccccgccga gcagctgggc ctcgtcgacg aggtcgtacg
      661 gatcaagccc gatgcgatcg tgttcgcgcc gttcgatccc aaggcgatgg tgccagcggt
      721 cgacaagctc aacgcagccg gcatcccggt gaccaacgtc aacgaacggc tcgccggcgg
      781 caatgtcgtc gcttacgtcg gaaccgacga ctatcagctc gcgctcacga ccgcgcgcta
      841 cctcatcaac gcgatgggca agaagggaaa cgtcgtgatc ctcgaaggcc ccgacaacct
      901 gccgaccagc atcgcccgcg tgaaggctta caaggacgcg ctcaaggaat ttcccgacgt
      961 caagctcctc gcctcgaagt cggcgaacta cgcccgcgcg ccggcgatcg aagtgatgaa
     1021 gagctttctg cggctctatc cgcagatcga cggcgtgctc gccgccaacg atccgatggc
     1081 gatcggcgcc gtcgaagcgc tcaaagcggc caaccggaag gcaccggtcg tcggcatcaa
     1141 tgccagcaaa gaggtgatcg acttcgtcaa gtccggtgat atcctcggta gcggcgacta
     1201 caatggattc gttcaaggct gtctcggcac ccagatcgcc atccacaatt tgcgcaagca
     1261 gccgacgccg aaggaagtca acctgaaggc tgtcgtgatg gataagacca actatcagga
     1321 ctacgaaacg ccgatcgaac ggcggtcttg cccgacgttg gaaagtgtct cggccaatta
     1381 ggccgtgcac tcataaatcg aggctggcct gcaaccgccg gctaaattgt atttgtatcc
     1441 atcgtcattt caatatcaag ccgaccgatg gaataaacca gcccctcaag agatagacga
     1501 aaaccagcat gacactcagc ggaatgaccc cggtccagcc cgaccacacc cgttgggtcc
     1561 atcggatggg gaaaaagaag ctggcaatga ccgtagctac caacgctccg gtcgccggcg
     1621 tcgcccaatg cttcaagaat tcaagttcgt agctcgaccg atcgtagata aaaaccgcga
     1681 caccatttgc gatcacatag aacacggcgt aggtgcaagc gaacgtgatc gtccattgga
     1741 tccagcgctc ccaccgctcc gtaacgaagg tagcgaccgc ggccaccgcg agcgcaccaa
     1801 tggcagccgt cgtgatccag ccgtaccaat acatggccgg ccctgccttc gctggttgcg
     1861 ccagcaggtc gagttcgtgg agcagcggat gataggtgaa aagcgcgtag ttgaaagttg
     1921 ccgccagcaa atacagcacg gcaaacgcgg tcgagaacac gggaaaagca cgatagaaac
     1981 tcatgaccat agtccccgaa tgttattggt gaaatgtccc accaaaaaat tccaccagat
     2041 cgtgaaggca ataatggtgg tcagaaattt tcggatgctc gcccgttcgg ccgaaagagg
     2101 ctgcctccaa tagtaccaat aggcgggaag cagtcccaac gcgaccgttg aaaagtgctc
     2161 cttgaggtcg aagattccga cg
//
LOCUS       DATLJT010001284         1590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3090529,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001284 DATLJT010000000
VERSION     DATLJT010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090529"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VM910_29500"
     CDS             complement(<1..362)
                     /locus_tag="VM910_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66680.1"
                     /translation="MVDRKHFRSDEPDFDRYDDEDFDSTPTRPYAFGRSARTEYGSND
                     SIPLFLSDPDGEPDPLEYANPLRKRGGAAISLRILMGVLAASGVAVLFAWFTSDATRD
                     VLVSAKASLSTVLPIPSAA"
     gene            complement(731..>1590)
                     /locus_tag="VM910_29505"
     CDS             complement(731..>1590)
                     /locus_tag="VM910_29505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HVI66681.1"
                     /translation="NFFDILMIQGKYQIKPPFPFSPAAEVAGVVESVGAGVTDLKPGD
                     RVVASSGHNGAREKIALPAAAIVKIPDNLDFDRAAGIIIIYGTALHALEDRASPKAGD
                     TLAVLGAAGGTGLAACELGRLMGLKVIACASSEEKLEFAKAHGAELTLNYAKEDLKEG
                     LRRLTGGKGVDIIFDPVGGTYAEAALRSIAWEGRFLVIGFAAGDIPKMPLNLALLKGC
                     DIRGVFWGNWARLNPAKNRANLEKLVKWTAEGKISSHVDRTFPLAQTADALKVLAGRK
                     AMGKVILHP"
BASE COUNT          297 a          473 c          511 g          309 t
ORIGIN      
        1 gctgcggagg gaatgggaag cacggtcgaa agcgacgctt tggcgctgac gagcacgtcg
       61 cgcgtggcgt cggaggtgaa ccatgcaaag aggaccgcaa cgccggaggc tgcgagcacg
      121 cccatcagga tgcgcaacga gattgcagcg ccgccgcgct tgcgcaaggg gtttgcgtat
      181 tcgagcggat cgggctcgcc gtcaggatcg gagaggaaga gcgggatgga atcgttggag
      241 ccgtattccg tccgcgcaga gcgtccgaag gcatagggcc ttgtgggcgt gctgtcgaag
      301 tcttcgtcgt cgtagcgatc gaagtcgggt tcgtctgacc gaaagtgttt cctgtccacc
      361 atggtgattt ccccactcag cttgacgcaa caaatcgggg caccaggctc cctgttatgg
      421 cttgtttgct gtccggaagc gccccgttac tttagcctag attcgcaagc tgaatcagcc
      481 caaaagacgg ccacggtcgg cgcgaatggg gagatatttt ggattctttg cggcagcgcc
      541 cagtaatgtt tgcgcaaaac ctgaacgaaa gcggggtata gcgaggctat ttcgtggcgc
      601 atgcgtggca aaactgcgac cggatcgatg gtcggggttc atgctgtttc gaattgcgaa
      661 aagcagatgc gataaatcgc cgttagcgtg ctgacttagc ggctaaccac ctccgcgagg
      721 ctgacccgtt tcagggatgc aggatgacct tgcccatcgc cttgcgacct gcgagcacct
      781 tcaacgcatc cgccgtctgc gccagcggga aggtgcggtc gacatgggat gagatcttgc
      841 cttccgcggt ccacttcacg agcttctcga gattggcgcg gttcttggcc ggattgagcc
      901 gcgcccaatt gccccagaac acgccgcgga tatcgcagcc cttcaggagc gcgaggttga
      961 gcggcatctt cggaatatcg ccggcggcaa aaccgatcac gaggaatcgt ccttcccagg
     1021 cgatcgagcg cagcgctgct tcggcgtagg tgccgccgac gggatcgaag atgatgtcga
     1081 cgcccttgcc gccggtgagg cgccgcaatc cctccttgag gtcttccttg gcgtaattga
     1141 gcgtgagctc cgcgccatgc gccttggcga attccagctt ttcttccgac gacgcgcagg
     1201 cgatcacctt cagtcccatc agtcggccga gctcgcacgc agcaaggccc gtgccgccgg
     1261 ctgcgcccag caccgcgagc gtgtcgcctg ccttcgggct tgcgcgatcc tcgagcgcat
     1321 gcagcgcggt gccgtagata atgatgatgc cggcagctcg atcgaaatcg agattgtcgg
     1381 gaatcttcac gattgcggcg gccggcaatg cgatcttctc gcgcgcgccg ttatggccgc
     1441 tcgacgccac cacgcggtcg ccgggcttga ggtccgtgac gcctgcgccg acgctctcga
     1501 cgacgcccgc gacctccgca gccggcgaaa acggaaacgg cggcttgatc tggtacttgc
     1561 cctggatcat caggatgtcg aagaagttca
//
LOCUS       DATLJT010001285         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2400548,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001285 DATLJT010000000
VERSION     DATLJT010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2400548"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VM910_29510"
     CDS             complement(<1..727)
                     /locus_tag="VM910_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVI66682.1"
                     /translation="MPAIERVTPLVGYLPTSIREALVRRLRELMGLGLIVVTGLATAA
                     LATWSVHDPSLSHATSRGIHNMLGYGGAIFADLLMQILGLGAIMVILPMAVWGWRMMT
                     HRGFDREALRLGAWVLCMVTAAGFFSCWPRSGAWPLPTGLGGVVGDALVRAPAVVFGP
                     PGFVYRLVLGIMLLAAVVATFLIASGAGSRAVEEELTPIEDDDAPFVEEEEDDGSISL
                     GWAFHALMSAKARLVWLLSAAYRS"
     gene            complement(746..1960)
                     /locus_tag="VM910_29515"
     CDS             complement(746..1960)
                     /locus_tag="VM910_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI66683.1"
                     /translation="MAMTASSRVPSTASQKESERSPFARTTELLAPYKPGKPLITLSL
                     GEPQHPVPGFVGPVLAEHIAEFGRYPLARGIEPFRKAAAAWLSTRFKLPRPVDPESEI
                     LVLNGSREGLFFAALTAASYVGNRSGRPAILMPNPFYPAYGAGARAAACEQIYLPTTP
                     DNGFLPDLDALDEATLARTVAFYIASPANPQGSVASPAYFGRLKKLADRFGFMILSDE
                     CYSEIYTREAPGSMLECAGADFKNVVVFQSLSKRSNLPGMRVGFAAGDGRFLAAFHEL
                     RNVAAPQVPVPLQHVAVAAYGDETHVEDNRKLYRIKFDLADQILGNRYGYKRPAGGFC
                     VWLDVSRHGGDEAATVKLYREAGVRVIPGSYLARLQPDGFNPGAGYIRLALVSDSEQT
                     GEALHRLVEVLG"
     gene            complement(2166..>2365)
                     /locus_tag="VM910_29520"
     CDS             complement(2166..>2365)
                     /locus_tag="VM910_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HVI66684.1"
                     /translation="LHRHALMTMIAYAFLQHRRLVQARRKKKNQRASASAKLAGGTPS
                     HRRSHSSTATTTMPILQKTNP"
BASE COUNT          452 a          743 c          781 g          389 t
ORIGIN      
        1 gcgaacgata ggccgcgctc aacagccaga cgagccgcgc tttcgcgctc atcagtgcat
       61 gaaacgccca cccgagcgag atggatccgt cgtcctcctc ctcttcgacg aacggcgcat
      121 cgtcatcctc gatcggcgtc aactcctcct cgacagcgcg cgagcctgcg ccgcttgcga
      181 tcaggaaggt cgcgaccacc gccgcaagca gcatgattcc aagcacgagc cggtacacga
      241 agccgggcgg cccgaacacg accgcgggcg cgcgcaccag cgcgtcgccg accacaccgc
      301 cgaggccggt cggcaagggc cacgcgcccg aacgcggcca gcaactgaaa aagcccgcgg
      361 cggtcaccat gcacaagacc cacgcgccaa gccgcagcgc ttcgcggtcg aagccgcgat
      421 gcgtcatcat gcgccagccc cacacggcca tcggcaggat caccatgatg gcgccgaggc
      481 ccaggatctg catcaacagg tcggcgaaaa tcgcgccgcc atagccgagc atgttgtgaa
      541 taccgcgcga ggtggcgtgg ctcaggctcg gatcgtgcac cgaccaagtc gccagcgccg
      601 cggttgcaag gcctgtcacc acgatcaggc caagtcccat caattcgcga aggcgccgca
      661 ccagggcttc gcggatcgac gtcggcagat agccgaccag gggagtgaca cgttcgatcg
      721 ccggcatatg ctgtgcgccc cgcgattaac ccaaaacctc gaccagacgg tgtaacgctt
      781 cgccggtctg ttcgctgtcg gagaccagcg caagccggat gtagccggcg ccgggattga
      841 agccgtccgg ctgcagcctt gccaggtagc tgccgggaat cacccgcacg ccggcctccc
      901 gatagagttt cacggtcgct gcctcgtcgc cgccgtgccg tgacacgtcg agccagacgc
      961 agaagccgcc ggccggccgc ttgtagccat atcggttgcc gaggatctgg tcggcaaggt
     1021 cgaacttgat gcggtagagc ttgcggttgt cctcgacatg cgtctcgtcg ccataggctg
     1081 cgaccgcgac atgctgcagc ggcaccggga cctggggcgc ggcgacattg cgcaattcgt
     1141 gaaaggccgc aagaaagcga ccgtcgccgg cggcaaagcc gacccgcatg cccggcaggt
     1201 tcgaacgctt cgacagcgat tgaaacacca cgacattttt gaaatctgcg ccggcgcatt
     1261 cgagcatgct gcccggcgct tcgcgggtgt agatttccga ataacactcg tcgctcagga
     1321 tcatgaagcc gaagcgatcg gcgagctttt tcagacggcc gaaataggcg ggcgaggcga
     1381 cggagccttg cggattggcg ggcgaggcga tgtagaacgc aacggttcgt gcaagcgttg
     1441 cctcgtcgag cgcgtcgagg tcggggagga atccgttgtc cggtgtggtc ggcagataga
     1501 tctgctcgca ggcggcagcg cgggcgcccg cgccataggc gggataaaac ggattgggca
     1561 tcaggatcgc cggccgcccg gagcggttgc cgacatagct cgccgccgtt agtgccgcga
     1621 agaacaggcc ttcgcggctg ccgttcagca ccagaatctc gctttcggga tcgaccggcc
     1681 gcggcagctt gaagcgggtc gaaagccagg cggctgccgc cttgcggaac ggctcgatgc
     1741 ctctcgccag cgggtagcgc ccgaactctg cgatatgctc cgcgagcacc ggtccgacaa
     1801 agccgggtac ggggtgctgc ggctcaccca atgacagcgt aatcaagggc ttgccgggtt
     1861 tgtacggcgc cagaagctcg gtcgtccgtg caaagggcga gcgttcgctt tccttctggc
     1921 tggccgtcga ggggacgcgg gatgaagcgg tcattgccat aagttaagac gccagcactc
     1981 tgggggtcgc cgcgcatgtg agccacggcg cgaaagcgga tcagaccacc atagatacgg
     2041 cgaggttaag acgcgattaa ccatcgaggc cagcgaagat tttgcttgcg cgtggtgccg
     2101 gcgaggccgc gactaacgga cccgcggcct agcactactt tggcagattg atttgctgtg
     2161 gcttttcacg ggttcgtctt ctgcagtatg ggcattgttg tggtggcggt cgaagaatga
     2221 gatcgacgat ggcttggcgt accgccggca agcttggctg aggcggaggc ccgttgattc
     2281 ttttttttcc gcctcgcctg aacgaggcga cggtgttgca agaaggcgta ggcgatcatg
     2341 gtcatcagcg cgtggcgatg aaggc
//
LOCUS       DATLJT010001286         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2632192,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001286 DATLJT010000000
VERSION     DATLJT010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632192"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VM910_29525"
     CDS             complement(<1..695)
                     /locus_tag="VM910_29525"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HVI66685.1"
                     /translation="MSLRAELVRLGLRWCVKRKIGPATTLEALRRQDAFLERLVPPPP
                     KGTHATHVDLDGVKAVAIVTPASHGGRLVLYLHGGGHVSGSPALYSDFTWRIAAATRA
                     RTVILDHRLAPEHPFPAALNDAVTAYRALVADGTDPRRMAVLGESSGGGLVLAMLLKL
                     RDEGFHLPAAAVALSPWTDLALTGNSLQRNAEADPMLRGTEGARLASYYLAGADPRTP
                     YASPLYADPTGLPP"
     gene            complement(996..1928)
                     /locus_tag="VM910_29530"
     CDS             complement(996..1928)
                     /locus_tag="VM910_29530"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HVI66686.1"
                     /translation="MRIAVTGAFGFCGKYIAQRLLALRHEVVTLTNSPQRANPFGGSI
                     EAFPYNFSEPHELEKSLRGIDALINTYWVRFDNPPHFTFAQALANSKVLFDAAKRAGI
                     GRVVHISITNPDRKSRLPYFRGKAESEDYLKQTGISYAILRPAVLFGKEDILINNIAW
                     TLRRLPVFGIYGDGQYRLQPIYVDDLAVAAIDRITGPANETVDAIGPETYYYREMVEM
                     IAREIGSPALIVSMPPMLAYQAVGVLGWIIGDVINTRDEVRGLMQEKLYVRSPPLGTT
                     KLSDWVCASRQDIGRRYASELRRRLDRGTAYRTQ"
     gene            2364..3356
                     /locus_tag="VM910_29535"
     CDS             2364..3356
                     /locus_tag="VM910_29535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66687.1"
                     /translation="MKKAITRRRLVELTAAFAASSAVAHNVQAQAYPNRTIRLIAPFP
                     AGGGTDSAARIIAARLSELLGQQVVVDNRAGAGSNIGAEAAARSTPDGYTLLLGAPPL
                     AINRFLYASLNYDSVTDLAPVSLLCRFPNILAVPVSSPLTSVRSFIDYAKANPGKVTY
                     SSPGIGTTPHLSGELFKRMAGIELIHVPYRGAGAGAITDTIAGRVDSAINTTGSLLPT
                     VRSGQLRGLAVTTLKRFPTAPELPTIAESGIPGFDVSSWYALFVPAKTPPEIIARLNA
                     TTVTALSEPAVRARFEPLGVVVESSTPEGLGALLQSEIDKWGPIIKAAGISASN"
     gene            complement(3492..4232)
                     /locus_tag="VM910_29540"
     CDS             complement(3492..4232)
                     /locus_tag="VM910_29540"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI66688.1"
                     /translation="MACPFGLFYWPDGWVGREKDVRDVTALCEKFGIDNPLANLRGLR
                     LAVPSTVFQNDLSPAVVDSFRAALTRLSSAGATVVGLPMAEFAQAAAVKSRGALTSAE
                     AYSWHRPWMKDGADKYDPRVIVRIRPGETMTAANYIELMKLRAQFIRTINAAASGYDA
                     MLMPTTPDTAPTIAEASKDDETYSRLNLRMLRNPAIVNLFDGCALSVPCHEPGAAPVG
                     LMIAGTLNTDRKILEVGLAVEQVVSRRG"
     gene            4268..5179
                     /locus_tag="VM910_29545"
     CDS             4268..5179
                     /locus_tag="VM910_29545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HVI66689.1"
                     /translation="MAEPVSPLVAFSDHVALLVERASASIVAVHGGGRWSSSGIHWRP
                     GVIVTAEEVLERDDELTVTLPGGRKVAASLAGRDPSTDVAALRIEAQSLPIAATADAG
                     SLRPGHVILAVGSFEDAPAASFGLVGFVGSAWQSLRGGTIDSLVRLDLALDARAEGGA
                     VVDSRGRVVGMAVSGPRRRVLVIPTATIDRSVDQLLAKGFVGRGYFGARLQHVRLARQ
                     RNGAQSLEREHGILIVSIDRDGPAAHAGLVIGDIVTAWNAQPLTRVRDVMQRLGPDSI
                     GKTIDLQLLRGGAPKDLKIVIGERPLA"
     gene            5234..>5421
                     /locus_tag="VM910_29550"
     CDS             5234..>5421
                     /locus_tag="VM910_29550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66690.1"
                     /translation="MVAVAIYSADPADRLQLEQQLRAEPGYRVAGVTHDPTTVSRLIE
                     QSRADTVVAHAPSREQLT"
BASE COUNT          985 a         1691 c         1720 g         1025 t
ORIGIN      
        1 ggcggcagtc cggtcgggtc ggcgtaaagc ggcgacgcat acggggtgcg aggatcggcg
       61 ccggcgagat aatagctcgc gagccgcgcg ccctccgtgc ctcgcagcat cggatcggcc
      121 tcggcgtttc gctgcaagga attgccggtg agcgcgagat cggtccaggg cgaaagcgcg
      181 acggcggcgg ccggcagatg aaagccttcg tcacgcagct tgagcagcat cgccaagacg
      241 agtccgccgc ctgaggattc tcctaggacc gccattctcc gcggatcggt accatcggcc
      301 acgagcgccc ggtaggccgt gacggcatcg ttgagcgcgg ccggaaacgg atgctcgggc
      361 gcaagccgat gatctaggat aacggtacgg gcgcgcgtcg ccgccgcgat acgccaggtg
      421 aaatcactat agagcgccgg cgagcccgac acatgcccgc cgccgtggag atagagcacg
      481 aggcggccgc cgtgggacgc gggagtaacg atggcgaccg ctttgacccc gtcgaggtca
      541 acgtgtgtcg cgtgcgtacc ctttggcggc ggcggcacta gacgctcgag gaatgcgtcc
      601 tgtcggcgca gtgcttcgag ggttgtcgcg ggaccgatct tgcgcttgac gcaccagcgc
      661 agccccaggc gcacgagttc ggcgcgtaga ctcatcgcgg ccccgctcca ctgcgttggg
      721 taccggcaag aagctcacga ttgttgcccc acatcgagct cccacgcttc cccgaccagt
      781 gtctttccga aatcatcgtg ccctttcgtg gcgacgagct tgaattcggc gccgcatcgt
      841 agatgcggta ggcggacccc accttcgatc accccaccag ccatcgaagg tccgtagggc
      901 ggcccggctc aagcggtatc attgcatgcc aatgcagttt gcagcgccgc tttccagctg
      961 tactggcctc aggtcgataa tcgagtcgcc gcctttcact gggtacggta ggcggtgcct
     1021 cgatcgagtc ggcgccgtaa ttcgctggcg tatcggcgcc ctatgtcttg gcgtgacgcg
     1081 cacacccaat cactgagctt ggtcgtgccg agcgggggtg atcgcacgta gagcttttcc
     1141 tgcattaggc ccctaacctc atcgcgcgtg ttgatcacat ccccgatgat ccagccaagc
     1201 acgccgacag cttggtaagc gagcatcggc ggcatcgaca cgatcagggc gggcgacccg
     1261 atctcgcgcg caatcatttc caccatctcg cggtagtagt aggtttcagg cccgatggca
     1321 tcgactgttt cgttggccgg tccagttatg cggtcgatag ccgccaccgc aaggtcatca
     1381 acgtagatcg gctgcagccg atattgcccg tcgccataga tgccgaacac gggcaaacgc
     1441 cgcagggtcc aggcaatgtt gttgatcagg atatcttcct ttccgaacag cactgcaggc
     1501 cgcaggatgg cgtatgagat gccggtctgc ttgaggtagt cctccgattc cgccttgccg
     1561 cggaagtagg gaagacgcga tttgcgatca ggattagtaa tgctgatgtg cacgacgcga
     1621 ccgatgcctg cgcgcttggc agcgtcgaac aaaactttcg agttggcgag tgcctgggca
     1681 aaagtaaaat gcggcggatt gtcgaaccgt acccaatagg tgttgatcag agcatcaatg
     1741 ccgcgcagcg atttttctaa ttcatgcggt tcggagaaat tgtatggaaa cgcctcaatc
     1801 gatccgccaa acggattggc tcgctgcggc gaattcgtaa gtgtgaccac ctcgtgtcgg
     1861 agcgcgagca agcgctgtgc aatgtacttg ccgcagaagc caaaagcccc tgttaccgcg
     1921 atccgcacgc tggctttcca tcacaccgag aagattacgc actcggctcg ctataggaac
     1981 tcttccaacg gatggctgtt ccctgcgaac cgcctgcgct cgatgactgg caaggatggc
     2041 aatgaacgcg cccggctctc cgcgcctaga ttttcgctag ccgacagcca cgaggctgct
     2101 gcagtcgcca tccagacctg ggtgatcgga acccacggag gcgtgcgaag agtcgccagc
     2161 gggttcccga tcgccgcgcc ggcgggacca tcaccggttc ttacggcagg gtgccaaagt
     2221 cgagcgtgcg ctgcccaatc gcacttcgcg tgacggggac ggtgtcgatc tgacgaggcg
     2281 aggcagcgac cgacgatgaa gcttgttaaa tgtgctacta tgcgcgctgg cgagattgcc
     2341 atttcagaat tgcgtcgcgc tccatgaaaa aagcgattac ccgccgccga ctggtggagc
     2401 ttaccgcggc gtttgcagca tcgagcgcag tcgctcacaa cgttcaagcg caggcttacc
     2461 cgaaccgcac cattcggctg atcgcgccgt tccctgccgg cggtggcacc gactccgcgg
     2521 cccgcatcat cgctgctcgc ttgtcggagc tgctgggcca gcaggtagtg gtcgacaacc
     2581 gggctggcgc cggcagcaac atcggcgccg aggccgccgc gcgctcgacg cctgacggct
     2641 acaccctgct gctcggtgcg ccgccgcttg cgatcaaccg ctttctctat gcttcactca
     2701 actacgattc cgtcaccgat ctcgcgccgg tgtcattgct ctgccgcttt ccgaatatcc
     2761 tcgctgtgcc ggtgtcgtcg cctctgactt cggtccgcag tttcatcgac tacgccaagg
     2821 ccaatccagg caaagtgact tattcttcac cggggatcgg caccacgccg cacctgtccg
     2881 gtgaattgtt taagcgcatg gccggcattg agctcatcca cgtgccctat cgcggtgccg
     2941 gggccggcgc cattaccgac accatcgccg gccgcgtcga ttcggctatc aacactaccg
     3001 gctcgctgct gccaaccgtg cgctccgggc aactgcgcgg tctcgccgtc acgacgctga
     3061 aacgtttccc gaccgcgccg gaactgccga ccattgcgga gtccgggatt cccggctttg
     3121 acgtgtcgtc ctggtatgcc ctgttcgtgc cggctaagac cccgcctgag atcatcgcca
     3181 ggctgaacgc cacgaccgtc acggcattat ccgaacccgc ggtgagggcc aggttcgagc
     3241 cactcggcgt cgttgtcgaa agctcgacgc ctgaagggct cggagcgctg cttcagtctg
     3301 aaatcgacaa gtggggtcca ataatcaagg ccgccggcat cagcgcaagc aattgagcat
     3361 gagtcggcaa tgaggctcac gctgagcaga tgatttctga ttaacccttg taagccagac
     3421 atccagttgg caagtcacaa gcgatcttgt tgcaaaagtc attgatggtt tccgtgcgcg
     3481 ttggtgctct atcagccgcg ccgtgaaacg acttgctcca ctgcgagacc gacctccagg
     3541 atcttgcggt ccgtgtttag cgtgccggcg atcatcaaac cgacgggtgc ggcacccggc
     3601 tcatggcagg gaaccgacag ggcgcagccg tcgaacagat tcacgattgc cggattacgt
     3661 agcatgcgca ggttgaggcg agaatatgtt tcgtcgtcct tgctggcttc ggcgatcgtt
     3721 ggcgccgtgt ccggggtggt cggcatcagc atggcatcgt aaccgctggc ggcggcgttg
     3781 attgtgcgga tgaactgcgc gcgcagtttc atcagttcga tgtagtttgc agccgtcatc
     3841 gtttcaccag gacggatgcg gacgatgacg cgcgggtcgt atttgtcggc cccatctttc
     3901 atccacggtc ggtgccacga ataggcttcg gcactggtca acgcaccgcg tgacttgacg
     3961 gccgcggctt gggcaaattc ggccatggga agcccgacca cggtcgcgcc ggcgctcgac
     4021 aagcgggtga gagccgccct gaagctgtcg acgaccgctg gcgacaggtc gttctggaac
     4081 accgtgctgg gcaccgcgag acgcaggccg cgcagattgg caagaggatt gtcgataccg
     4141 aatttttcac agagggcagt tacgtctcgc acatccttct cgcgcccgac ccacccatcc
     4201 ggccagtaga agaggccgaa cgggcatgcc atatgcggcc cgagcatctt ctatggaggt
     4261 cttcatcatg gccgagcccg ttagtcccct tgtggccttc tccgatcacg tcgccctcct
     4321 tgtcgagcgc gcgtccgcga gcatcgtcgc agttcatggc ggcggccgct ggtcttcgag
     4381 cggtatccac tggcgccccg gtgtgatcgt cacggccgaa gaggtactcg agcgcgacga
     4441 cgagcttacc gtgacgctcc cgggcggacg gaaggtcgca gcctcgctcg cgggtcgtga
     4501 tccgagcacc gacgtggcag cgttgcgcat cgaggcacag agcttgccga tcgcagccac
     4561 cgcagatgcc ggatcgctgc ggccgggcca cgtgatcctt gcggtcggaa gctttgagga
     4621 cgcgcccgcc gcgagctttg ggctggtggg tttcgtgggc agcgcctggc aaagcctgcg
     4681 cggcgggacg atcgacagcc ttgtgcgtct tgatctggcg ctcgacgcac gagccgaggg
     4741 cggtgctgtg gtcgactcgc ggggacgggt cgtcggcatg gcggtcagcg gaccgcgccg
     4801 acgggtactt gtcatcccga cggcgacgat cgaccggtcc gtcgaccagc tcctggccaa
     4861 gggatttgtc ggccgcggat atttcggcgc cagacttcag catgtgcgtc tcgcgcgcca
     4921 acgaaacggg gcgcagtcgc tggaacgcga gcatgggatc ctgattgtga gcatcgaccg
     4981 ggatgggccc gcggcgcacg cggggcttgt gatcggtgac atcgtcacgg cgtggaacgc
     5041 gcagccgctg actcgggtgc gggatgtgat gcaacgcctc gggccggata gcatcggcaa
     5101 aaccatcgat ctgcagctct tgcgcggcgg agccccgaaa gacctcaaga tcgtgatcgg
     5161 cgaacgcccg ttggcataaa tgtctcgtcg tgcacagggc gcggctgcgt cattgatggg
     5221 accggggaca tccgtggtcg cggtcgcaat ctactcggcc gatcccgcgg accggctgca
     5281 gcttgaacag cagttgcgcg cagaaccggg ctatagagtt gccggcgtca cccatgaccc
     5341 gacaacagtg agccgattga tcgagcaaag tcgcgccgac acggtggtag cgcatgcacc
     5401 gtcgcgcgaa cagctcactg a
//
LOCUS       DATLJT010001287         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1021352,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001287 DATLJT010000000
VERSION     DATLJT010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021352"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VM910_29555"
     CDS             <1..621
                     /locus_tag="VM910_29555"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HVI66691.1"
                     /translation="VLNFRLNWEGNFRSPAAQLVSLLENPVNMRSSVEDVVRKLKADP
                     GIVDQFRAAYGGEPDRERFLDAMVTFERSLVTPGSRFDRWLAGDRSALSQAELEGYRL
                     FKSVGCSSCHQGVNVGGNLFERQGIFHPLVQRPPEIVRVPSLRNVATTAPYFHDGSAA
                     TLHDAVRRMAASQLDRTLTDQQVDSLVAFLRTLTGNYRGSPVGAAP"
     gene            666..>2635
                     /locus_tag="VM910_29560"
     CDS             666..>2635
                     /locus_tag="VM910_29560"
                     /inference="COORDINATES: protein motif:HMM:NF010411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system VirA-like sensor kinase"
                     /protein_id="HVI66692.1"
                     /translation="MSWLSVRATNPEAEMFDRALAEIDRFAVLENELYRDVFLARTGT
                     LHNYDPVVREIDALHDAIDRLRSTAAIDAETKTAVDRLAASVDRQEQLAEHFKSENAL
                     LHNSLSFFVRFSARQASADLNPAISAAAAAMLQLTLDTSPASAQQLKDQLDQLSQQPA
                     LRADAAATLEALVAHGLLLHDLLPSVDNTLRTMRAEPRKLDQDALRTLIMTRETASRN
                     LAREYRQVLYVTSLLLVAFLVHLGLKLKSRANALQRRAAFEHLIARISMRFINARPQD
                     IGGEIDRAIADMAACIGSDRAYFVMSGSTQRVHLWCKPGTNAPAHWPVYVAALATQFD
                     QTADGIVHVPQVDRLPFGEVRSALVRVGVCGWACVTNTGKDGTVASLGFDAIGRACRI
                     TAPGELSLLRMALDTILYAVARHSMETERARLESRLRQARRLERIGTFTSGIAHNFNN
                     LLGGILGHSEVMEERLGSDTRVARNLAAIRRGAESAHDLVDQILAFGRHRDVRPRPLN
                     ANALITEATALFKVSLPVGIQVAVHEPPVAAIVSGEPAQLQQVLLNLCSNAAQAVEGS
                     GRIDIGMEVKDLTTAQRFSHAELQPGRYACITISDTGHGMDEATLARIFEPFYTTRSS
                     GNGLGLATVHEIVRDHGGGINVSSAPGQGSHF"
BASE COUNT          496 a          826 c          807 g          506 t
ORIGIN      
        1 gtcctgaatt ttcgactgaa ttgggaaggc aacttccggt ccccggcagc gcaactcgta
       61 tcgttgctcg aaaaccccgt gaacatgcgt agcagcgtcg aggatgtggt ccgcaagctg
      121 aaagccgatc ccggaatcgt ggatcaattc cgtgccgcat acggaggcga gcccgacagg
      181 gagcggtttc tcgacgcgat ggtcaccttt gaaagatcgc tggtaacgcc gggatcaaga
      241 tttgatcgct ggctcgcggg cgaccggtcg gcactttcgc aggcagaact cgaaggttac
      301 cggttgttca aatcggtcgg ctgtagttcc tgtcatcaag gcgtcaatgt cggcggaaat
      361 ctgtttgagc ggcagggcat cttccatccg ctggtccagc ggccgccgga aatcgtgcgt
      421 gtgcccagcc tgcgcaatgt ggcaacgaca gcgccctatt tccacgatgg cagtgccgcg
      481 acgctgcacg acgccgtgcg caggatggcc gcctcgcagc tcgaccgcac ccttaccgac
      541 cagcaggtgg actcgctcgt cgcctttctg cggactctga ccggtaatta ccgcggctcc
      601 cccgtcggag ccgcgccgtg agggcggcgc ccaccgcatc gatcgttgcc tgtctggtcc
      661 tgctcttgag ctggctttcg gtgcgcgcga ccaatccaga ggccgagatg ttcgaccggg
      721 cgctcgccga gatcgaccgt ttcgcggtac tggaaaacga gctgtatcgc gacgtcttcc
      781 tggcgcgtac cggcacgcta cacaattacg acccggtggt cagagagatt gatgcgctcc
      841 acgatgcgat tgatcgtctg cgcagcacgg cagcgatcga cgctgagacg aaaacggccg
      901 tggatcggct tgccgcatcg gtggatcggc aggaacaact ggccgagcac ttcaaaagcg
      961 agaacgcgtt gctgcacaat tcgttgtcgt tctttgttcg atttagcgca cgtcaggcgt
     1021 cggccgacct gaatccggcg atcagcgcag ccgccgccgc aatgctgcaa ctcacgctag
     1081 atacgtcacc tgcgtcagcg cagcagctga aagaccagct tgatcagctc agccaacagc
     1141 ccgctctccg ggccgatgct gctgcaactc tcgaagctct tgtggcgcac ggactgctgc
     1201 tgcatgacct gctcccctcg gtcgataata cgctgagaac catgcgcgcg gagccgcgca
     1261 aactcgacca ggatgcgctt cggaccctga tcatgacacg cgaaacggct tcaaggaacc
     1321 tggcgcgaga gtaccggcag gttctctacg taacatcgct gttgctggtc gccttcctgg
     1381 tccacctcgg cctcaagctc aaatcacggg ccaacgctct gcagcggcgc gccgccttcg
     1441 aacatttgat cgcacgtatc tcgatgcggt tcatcaatgc gcgcccacaa gacatcggcg
     1501 gcgagattga tcgggctatt gccgatatgg cggcgtgcat cggctccgac cgcgcctatt
     1561 tcgtgatgtc tggctcgaca cagcgcgtgc atctctggtg caagccggga acgaatgctc
     1621 cggcccattg gccggtgtat gtcgccgcgc ttgccactca gtttgatcag accgcagacg
     1681 gcatcgtcca cgttcctcag gtcgaccggc tgccgttcgg cgaagtcaga agcgcgctcg
     1741 tcagagttgg tgtctgcggc tgggcctgcg tgaccaacac tggcaaggat ggtacggtcg
     1801 cctcgcttgg ttttgacgct ataggccgcg cgtgccgtat caccgcgccg ggcgaactca
     1861 gtctcctgcg catggcactc gacaccatcc tctacgccgt cgcgcgtcat tcgatggaaa
     1921 cggagcgcgc gaggctggaa agccgcttgc ggcaggcgcg ccgcctggag cgcatcggga
     1981 ccttcaccag cggcatcgcc cacaacttca acaatttgtt gggcgggatt cttggccatt
     2041 cggaagtgat ggaggagcgc ctgggctcag atacgagggt ggctcgcaac ctcgccgcga
     2101 tccgtcgagg agcggagagc gcgcacgatc tggtcgatca gatcctggct ttcggacgac
     2161 accgcgatgt gcgcccgcgg cctttgaacg caaatgccct gatcaccgag gcgaccgcac
     2221 ttttcaaggt gtcgctgccg gtaggaatcc aggtggccgt tcatgagccg ccggtggccg
     2281 ccatcgtttc cggcgaaccc gcccagctgc agcaggtgct tctcaatctc tgtagcaatg
     2341 ccgcgcaagc ggtcgagggg agcggacgca tcgacatcgg tatggaagtg aaggatctca
     2401 caaccgcgca gcgcttttcc catgccgagc tccagccggg gcgctacgcc tgcatcacca
     2461 tcagcgatac cggacacggg atggacgagg caacattagc gcggattttc gagccattct
     2521 acacgacgcg ttcctcagga aacggtctcg gattggcgac ggtgcacgag attgtgcgcg
     2581 accatggcgg cggcatcaac gtaagcagcg ctcccggcca aggtagccat ttcga
//
LOCUS       DATLJT010001288         3964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_333091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001288 DATLJT010000000
VERSION     DATLJT010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VM910_29565"
     CDS             complement(<1..1082)
                     /locus_tag="VM910_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HVI66693.1"
                     /translation="MANAFFSDLLATISERGRTLLRGAGPSDGKQDADGLIELCEALL
                     SGRGEASGTAMAREVLDQYHELDGEGQLSFFRALVRDFGPDREKLAEAIAVWQAQPGD
                     GNGTLLHFASEPRRQELIRRLNRAPGGTSELVSMRSDLLTLMDGHKDLAALDRDVVHL
                     LGSWFNRGFLVLRRIDWSTPANILEQIIRYEAVHEIRDWDDLRRRIDPADRRCYAFFH
                     PALADAPLIFVEVALTETIPSAIAPLLAVDREPVAIERARTAVFYSISNTQRGLGGIS
                     FGNFLIKQVVEELRRELPKLDTFVTLSPVPGFMPWLTEAKDVPLSDDDRALLKHLDEP
                     TWFEDAHTTALLRPVLEPLAAYYFLKA"
     gene            complement(1212..2012)
                     /locus_tag="VM910_29570"
     CDS             complement(1212..2012)
                     /locus_tag="VM910_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI66694.1"
                     /translation="MIIVEKPLSAVRGSHHATGDGVTMVAQAYRKGVRLDTHMHREAQ
                     LVYAARGTMQVTTPKGRWLVPPDRAVWVPARLAHAIDVLADIEMRTLYFDLAWLKRAR
                     RGTHLKSEFVVRVSPLLHQAILALFDRNSSPDRIALLVKLAMLELHQAEDSATFIPLP
                     HEPRCRRAADIVLRAPAASHEIERLARQVGTSARTLSRLFSAETQLSFKSWCQRARIA
                     AAIESLSTEANVSVKQLAADLGYASVPAFSHAFRQVTGRTPTEFVEKR"
     gene            2085..3266
                     /locus_tag="VM910_29575"
     CDS             2085..3266
                     /locus_tag="VM910_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66695.1"
                     /translation="MNSPSRVIGFVNLAHFIDHYSMLIFAAAVIIMGPALGMAYSELL
                     PYATPGFVAFGAGSLLTGWLGDRWSRRHMMVIFFLGIGASMIAVGLVQTPLQLGAALL
                     AIGIFASIYHPVGTAMIVSYADKLGREMGLNGVWGNLGVASSALVTGAIGQYLGWRFA
                     FIIPGIVTILIGVAFALTVMHEDRKGTRQAAAQARVAKEDMWRVIVALLIVVIAISTT
                     FNAVTVALPKLFAERLADMTRSPALLGAIAAGVYVFGAMTQYTIGKLLDRHSLKTVAL
                     PLSFMLAPFLYFAATLSNLPLILVSIGIVMGAFGQVTVNDAMVGKYTTEEWRSRAYAV
                     RYFVGFTAAGVSVGLVAWLYEQGGFVTMLHAFAGLCLFAIAAAIILPREIRTPAAQAM
                     Q"
     gene            complement(3298..>3964)
                     /locus_tag="VM910_29580"
     CDS             complement(3298..>3964)
                     /locus_tag="VM910_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI66696.1"
                     /translation="QGFSSGQSLKLMEQIADKTLPPGTGYEWTAMSYQEKAVTSQIYY
                     VFGLAMLLVYLVLAGQYESWFAPISVILAVPLSLLGPMLVLEALKIDNNLYVQIGLIL
                     LIALSAKNAILIVEVALELHVRDGKPLVESAVAAARARFRPILMTSFAFILGVVPLVL
                     ATGAGASARKSIGITVFSGMLASTCLAVLFVPAFFIVVQRFENWRSEKKTSAPKAAEQ
                     TGT"
BASE COUNT          698 a         1261 c         1285 g          720 t
ORIGIN      
        1 gctttcagga aatagtaggc ggcgagcggc tccagcaccg ggcggagcag ggcggtggta
       61 tgcgcatcct cgaaccatgt gggctcgtcg agatgtttca ggagcgcgcg atcgtcgtcc
      121 gacagcggca cgtccttggc ctccgtcagc catggcatga agcccggcac cggcgacagc
      181 gtcacgaagg tgtcgagctt cggcaattcg cggcgcaatt cctcgaccac ctgcttgatc
      241 aggaagttgc cgaaggagat gcctccaaga ccgcgctggg tgttggagat cgaatagaag
      301 accgcggtgc gggcgcgctc gatcgccact ggctcgcgat cgaccgcgag cagtggcgca
      361 atcgccgagg gaatcgtctc cgtcagcgca acctcgacga agatcagcgg cgcgtccgcc
      421 agcgccggat ggaagaaggc ataacagcgg cgatcggcgg ggtcgatgcg acggcgcaga
      481 tcgtcccagt cgcggatctc atgcaccgcc tcgtaacgaa tgatctgctc gaggatattt
      541 gccggtgtcg accagtctat cctgcgcagc acgagaaacc ccctgttgaa ccaggacccc
      601 agcagatgca cgacgtcgcg gtcgagtgcg gcgagatcct tgtgaccatc catcagcgtc
      661 aagagatcgg agcgcatcga gacgagctcc gaggtccccc ccggtgcgcg gttgagccgg
      721 cggatcagct cctgccggcg cggctcggag gcaaaatgca ggagcgtccc gttgccgtcg
      781 ccgggttgcg cctgccagac tgcgatcgcc tcggcgagct tttcccggtc cgggccgaaa
      841 tcgcgcacca gcgccctgaa aaacgacaac tggccttccc catcgagctc gtgatactgg
      901 tctagcacct cgcgcgccat ggcggtgccg gacgcttcgc cgcggcccga caacagcgcc
      961 tcgcaaagct cgatcaggcc gtcggcgtcc tgcttgccgt cggagggtcc ggcgccgcgc
     1021 agcagcgtgc ggccgcgctc ggaaatcgtc gcgagcaaat cggagaaaaa cgcgttggcc
     1081 atgaccctta cggcgaatcc ggacggcgca gatgatcaca tcaatttaag gcgaaggcag
     1141 gcacaatcaa gcgcctccgc acccctcgaa tacccgcttt cgcccgccgt gacgattatc
     1201 tgttcggcct attacctctt ttcgacgaat tccgtgggcg tccggccggt cacctggcgg
     1261 aacgcgtggg aaaaggccgg gacgctggca tagcccagat cggcggcgag ctgcttgacc
     1321 gatacgttgg cctccgtcga caggctctcg atcgcggccg caatccgcgc acgctggcac
     1381 cagctcttga agctcagttg cgtctccgcg gaaaacagcc gcgacagcgt tcgggccgag
     1441 gtgccgactt ggcgggccag ccgctcgatt tcgtgggagg ctgcgggcgc gcgcagcacg
     1501 atgtcggcgg cgcggcggca acgcggttcg tgagggagtg ggataaaggt cgcggaatcc
     1561 tcggcctggt gcagctcgag catcgcgagc ttgaccagca gtgcgatgcg gtcgggcgag
     1621 ctgtttctgt cgaacaaagc gaggatcgcc tgatgcagga ggggagagac ccgcaccacg
     1681 aattccgatt tgaggtgcgt gccgcgccgc gcgcgcttga gccaggcgag atcgaaatag
     1741 agcgtgcgca tctcgatgtc ggcgagcacg tcgatcgcat gtgcaagccg cgccggcacc
     1801 cagaccgcgc ggtccggcgg caccagccag cgtcccttcg gcgtcgtcac ctgcatcgtg
     1861 ccgcgtgccg catagacgag ctgcgcctcg cggtgcatgt gggtgtcgag ccgcacgccc
     1921 tttctgtagg cttgcgccac catggtgacg ccgtcgccgg tcgcatggtg gctgccgcgg
     1981 accgctgaga gtggcttttc gacgataatc attggctgca tcccgtaagc acaaagccat
     2041 ataacctcat ccggtaactt tagggaatcg ctcgaggacc cgtcatgaac agcccgagcc
     2101 gggtgatcgg ctttgtcaac ctcgcccatt tcatcgatca ttattcgatg ctgatctttg
     2161 ccgccgccgt catcattatg ggcccggcgc tgggcatggc ctattcggag ctgttgccct
     2221 acgcgacgcc gggctttgtc gcgttcggcg cgggatcgct cctgaccggc tggcttggcg
     2281 atcgctggag ccgccgccac atgatggtga ttttctttct gggcatcggc gcctcgatga
     2341 tcgcggtcgg cctcgtgcag acgccgctgc agctcggcgc agccctgctc gcgatcggca
     2401 tcttcgcctc gatctatcat cccgtgggca cggcgatgat cgtatcctac gccgacaagc
     2461 tcggccgcga gatgggactg aacggcgtct ggggcaatct cggggtcgcc tcctcggcgc
     2521 tggtcaccgg cgcgatcggg caatatctcg gctggcgctt tgccttcatc attcccggca
     2581 tcgtcaccat tctgatcggg gtcgcctttg cgttgacggt gatgcacgag gatcgcaagg
     2641 ggacgaggca ggccgcagcg caggcgcgcg tcgcgaagga agacatgtgg cgggtgatcg
     2701 tggcgctctt gatcgtggtg atcgcgatct cgactacctt caatgcggtg acggtggcgc
     2761 tgccaaagct gtttgccgag cggctcgccg acatgaccag gagcccggcg ttgctgggcg
     2821 cgatcgcggc cggcgtttac gtgttcggcg cgatgacgca atatacgatc ggcaagctgc
     2881 tcgaccgcca ctcgctgaaa acggtcgcgc tgcccctgtc cttcatgctg gcgccgttcc
     2941 tgtactttgc ggcgacgctg tccaatctgc cgctcattct tgtctcgatc ggcatcgtga
     3001 tgggggcgtt cggccaggtt accgtcaacg acgccatggt cggcaaatac accaccgaag
     3061 aatggcgctc gcgcgcctac gcggtgcgct atttcgtcgg cttcactgcg gccggcgtct
     3121 cggttggact ggtggcatgg ctctacgagc agggcggctt cgtcaccatg ctgcatgcct
     3181 tcgcgggctt atgcctgttc gcgatcgcag ccgcgatcat cctgccgcgg gagatcagga
     3241 cgccggcggc gcaggcgatg cagtgatcga agcggtattg cgcgtcggta tccaagctca
     3301 cgtgcctgtc tgctcggcgg ccttcggcgc cgatgtcttc ttctcgcttc gccagttctc
     3361 gaaacgctgc accacgataa agaacgccgg cacgaacagc accgcaaggc aggtggaggc
     3421 gagcatgccg gaaaacaccg tgatgccgat cgacttgcgc gcgctggcgc cggcgccggt
     3481 tgcaagcacc agcggcacga caccgagaat gaaggcgaac gacgtcatca ggatgggccg
     3541 gaagcgcgca cgtgcggctg ccaccgccga ttcgaccaac ggcttgccgt cgcggacgtg
     3601 cagctcgagt gcgacctcga cgatcagaat tgcgttcttc gcggacagcg cgatcagaag
     3661 gatcaggccg atctggacgt agagattgtt gtcgatcttc agcgcctcga gcaccagcat
     3721 cggcccgagc agcgacagcg gcacggccag gatcacagag atcggcgcaa accagctttc
     3781 atattgtccg gcgagcacga gatagaccag caacatcgca aggccgaaca cgtagtagat
     3841 ctggctcgtg accgccttct cctgatacga catcgccgtc cattcgtagc cggtgcccgg
     3901 cggcaacgtc ttgtccgcga tctgctccat cagcttgagc gactggcccg aactgaatcc
     3961 ctgc
//
LOCUS       DATLJT010001289         7668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_102351,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001289 DATLJT010000000
VERSION     DATLJT010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102351"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VM910_29585"
     CDS             complement(<1..302)
                     /locus_tag="VM910_29585"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HVI66697.1"
                     /translation="MAVLQIYGIERLVDIRTIPRSRHNPQFNDTALAKSLRAQHLEYM
                     HMQALGGLRHARKDSPNTGWRNASFRGYADYMQTEEFQDALEGLIHLSRRKRVAIM"
     gene            complement(428..1546)
                     /locus_tag="VM910_29590"
     CDS             complement(428..1546)
                     /locus_tag="VM910_29590"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HVI66698.1"
                     /translation="MATPAEAQSSSGSSSLGARERATRFCRRFGLRVPILQAPMAGAC
                     PVALAISVANAGGMGGFGALTTSPDGIADWVREFRDQSNGGFQINLWIPDPPPVRDPE
                     NEREVREFLAKWGPPVPAQAGDAAPLDFAAQCEALLAAAPPVVSSIMGLFPDKFIAEI
                     KARSTAWFACVTTLAEGRAAVAAGADAIVAQGSEAGGHHGAFDAAAAERQSVGLFALV
                     PRLADKLSVPIIATGGIGDARGIAAALTLGASAVQIGTAFLRCPEAGTNPAWADALAE
                     LEPEATMPTRAFSGRLGRAIATDYVRAAAAPGAPPPAPYPVQRALTAAMRDAAGRSRD
                     IHRMQAWAGQAAALARAEPAGELVLRLWREAEALLPVG"
     gene            complement(1673..2131)
                     /locus_tag="VM910_29595"
     CDS             complement(1673..2131)
                     /locus_tag="VM910_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI66699.1"
                     /translation="MSLPIDIVDRFCLTLVREAPDAIIFADAEGIIRFWNRGAERIFG
                     FAEAEALGQSLDIIIPDNLRVRHWAGFSATTRSGSTRYGSGEILAVPALRKDGRRISV
                     EFTILPFRDNHARIIGIAAMLRDVTARFEEIRTLRRQVAQRGRDENSPSS"
     gene            complement(2196..2498)
                     /locus_tag="VM910_29600"
     CDS             complement(2196..2498)
                     /locus_tag="VM910_29600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66700.1"
                     /translation="MNRRFDEAANAARRAAQGNPGFSIMHMPLAPSLAAQGRVTEANA
                     AARVLALQPDFTIDKHCADIAVLATLAVPLPKPAAPRDCRNHAPSQPRLLAGKEQV"
     gene            complement(3624..4023)
                     /locus_tag="VM910_29605"
                     /pseudo
     CDS             complement(3624..4023)
                     /locus_tag="VM910_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262867.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
     gene            4743..6227
                     /locus_tag="VM910_29610"
     CDS             4743..6227
                     /locus_tag="VM910_29610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66701.1"
                     /translation="MAAHDDHRNGNGCHHVLLIRAPPEAIRFAALAAIAILAFLWLGR
                     GLPRIPWASSDSESYLQYSPVRPHGYSLLLSGYRVIFTDLAHLPSVQLVLFVGAVLLL
                     AVAVQRRTRNFAAAVATLVVIFAATDTTSFPYVLSDPIYAAALTTGIAGFILYSDTPR
                     PGFLLLASAGLGVAVTFRAIGLALLPGFFLAIVLDRIGRRRGLVSAAAVGTLPIAALC
                     LAAASSQLIHNGRFALGSWGGMDVLGKVPLLARAVPEANGRLDGFVKAMELGRSKLRQ
                     LNPFVEALAARQYYEFLRWYVIIPELERCWPDWRDADDYRRGELAAELAKAYIAQDKL
                     GFARRTAIDLAGLWAMPRWLTDREREAALAEIEAVGELPGLTAFLRTPEAQLEYYKIV
                     PYASDPTQIAIFRLTVASFWVLSLGLIAIVLIPRLRAAMMTPDLVLILVTVHAIYIGT
                     ALMECAETRYIMPTWPTLVAGPILAFWLFRRLRSETPSPGCGWL"
     gene            6614..7668
                     /locus_tag="VM910_29615"
                     /pseudo
     CDS             6614..7668
                     /locus_tag="VM910_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
BASE COUNT         1365 a         2394 c         2446 g         1463 t
ORIGIN      
        1 cacatgatcg caacgcgttt gcggcggctc aggtgaatga gtccttcaag cgcatcctga
       61 aactcctcgg tttgcatata atcggcgtag ccgcgaaagc tcgcatttcg ccagccggtg
      121 ttgggggaat ctttgcgagc atgccgcaga ccccccagcg cttgcatatg catatattcg
      181 agatgctgcg ccctcaggct cttggccaag gcggtgtcgt tgaattgcgg attatggcgc
      241 gagcggggga ttgtgcggat gtccacgagc cgttcgatgc catagatttg caggacggcc
      301 ataaagcgct ctatcggcaa cgttgaatgg ccgagtgtga aaatagcgcc ctccggccaa
      361 tgcgcttgtt gcggcggaag atgttcgctc atggcgacgg gggtgagcgc gtgggccgca
      421 gcttcgccta gcctaccggc agcaaggctt ccgcctcgcg ccagagacga agcacgagtt
      481 caccggcggg ctcagcccgc gccaatgctg cagcctggcc tgcccatgcc tgcatgcgat
      541 gaatatcgcg tgacctcccg gccgcatcgc gcatcgctgc cgtcagcgct ctctgcaccg
      601 gatagggtgc cggcggcggc gcgccgggag cagcagcggc gcgtacataa tcggttgcga
      661 ttgcgcggcc gaggcgaccg gagaacgccc gcgtcggcat cgtcgcttcc ggctccagtt
      721 cggccagcgc atcggcccaa gccgggttgg tgccggcttc agggcagcgt agaaaggcgg
      781 tgccgatctg caccgcgctg gcgcccaacg ttagggctgc agcgatccct cgcgcatcac
      841 cgatcccgcc ggtcgcgatg atcggcaccg acaatttgtc cgcgagccgc ggcaccagag
      901 cgaacagccc gacggattga cgctccgcgg cggcggcatc gaacgcaccg tggtggccgc
      961 cggcctccga accttgcgca acgatggcgt cggcacccgc ggcgacggcc gcgcggcctt
     1021 cggccagtgt ggtcacgcag gcgaaccagg cggtgctgcg ggccttgatt tcggcaatga
     1081 acttgtccgg aaacagtccc atgatcgatg agacgaccgg tggtgctgca gcgagcagcg
     1141 cttcgcattg cgcggcaaag tcgagcggcg cggcatcgcc ggcttgcgcc ggcacgggcg
     1201 gaccccattt cgccagaaac tcccgcacct cgcgttcatt ttcgggatcg cgaaccggag
     1261 gagggtcggg gatccacagg ttgatttgaa atccgccatt gctctggtcc cggaactccc
     1321 ggacccaatc ggcgatcccg tcgggtgatg tggtcagcgc cccgaacccg cccatgccgc
     1381 ccgcatttgc cactgagatc gccagagcaa cggggcaggc gccggccatc ggcgcttgca
     1441 atatcggtac ccgcagcccg aagcggcggc agaaccgggt ggcgcgctct cgcgctccga
     1501 ggcttgatga gccggagctg gactgagcct cggctggtgt cgccatcgtg accctcgatg
     1561 cagttcgtgt cgactgaaga ttagcagatc tgacgcggcg ttggcctgcc caacatttgc
     1621 ccgatgctgg cgccttggcc cggagctggc cccgctggaa ggcctggcgc ggtcaactgc
     1681 tagggctgtt ctcgtctcgg ccccgctgag cgacttggcg ccgaagtgtc ctgatttcct
     1741 caaatcgtgc cgttacatcc cgcagcatag ctgcaattcc gatgatccgg gcgtgattgt
     1801 cgcggaacgg caggatcgtg aactcgacgg agatgcgccg gccgtctttg cgcagcgccg
     1861 gtaccgccag aatctcgccg gagccataac gcgtgcttcc gctccgcgtc gttgccgaaa
     1921 agccggccca gtgacgcacc cgcagattat cgggaataat gatatcgagg ctttggccga
     1981 gcgcctctgc ttcggcaaaa ccgaagatcc gttccgcgcc ccggttccag aagcgaatta
     2041 tgccctcggc atcggcaaag atgatcgcgt cgggcgcttc gcggacgagt gtaaggcaga
     2101 aacggtcgac gatgtcgatg gggaggctca ttatttcatt tcctgcccga ctatcgaggc
     2161 ggctccgaag ccggtgatcc gtaaacgggg attcatcaga cctgttcctt tcctgcgaga
     2221 agcctcggtt gcgagggagc gtggttacgg caatcccgcg gcgcggcagg cttcggcaag
     2281 ggtaccgcca gcgtggcgag gacggcgatg tcggcgcagt gcttgtcgat cgtgaaatcc
     2341 ggctgaagcg ccaggacgcg ggcggcggcg tttgcttccg tgacccggcc ttgcgcggcg
     2401 agggaaggcg ccagcggcat gtgcattatg ctgaacccgg ggttgccctg ggcggcgcga
     2461 cgcgccgcgt tcgccgcttc gtcaaagcgc cggttcatgt aatgtcgggg gagtgtcccc
     2521 ctcggccggg tagctatctg cggaaacaag ccgagctgca gtccggttcc cggttcacct
     2581 gacttgggag tgggcgtgtc gcgagagccg tgacgggaat ctcgtagaat tcttcgccaa
     2641 gtcaccgcta cacgaagagg ggctcgaaat cgaacgcccc gtgcaaccac ccgggcgaga
     2701 ccgaactttg acggactatc ttctcgaatg ggatgtctga accaccgggg tctaactcga
     2761 atgctaacgt cgcagcaatg ggtcagccgg aacatcgacc ccatctgccg cgccctcggc
     2821 tcgccctgct ggtgcatcgt gtcgcatgga gtccgactat cagccctgtc cggctcagtt
     2881 atcgtagcga tccccagctc ataagtatga aagatgaaat cattaaaaca acatataaag
     2941 ccgtaattgt accggcaata gttaaccgct cgaacaaaga ggcgcggtta attcgataat
     3001 tgagcatctt tctaccctca attgcgtcag tttttcttga aagaagatta atgacgtagc
     3061 cgccaaacaa taaaatgctc aatgagagca atgttgtccc aagcaccaga acagagtggg
     3121 cgatgtttgg catagcgcac gacttctctt gacccgtcga tatcggcaga tctagcgact
     3181 gccacgtcgg gtctggatcc aatattgaaa atgtcgcttg tcagaacggc caaagtcgag
     3241 cgcatcgatg gggaaaggcg attcgccgcg gtggcaaaga gcctctcctc gagcgtcgcc
     3301 caacgagaag gttcacgcga tcccgcggtt aaccaaaatg ggagcgatgg tcacagcgta
     3361 tccccgactg ctcgtcccgg cccgtccaag ctggacgtcc cgcgactgcc gcaatgcgac
     3421 tgctacgaac agataccccg tgtttcggca tatctagtgt gaactccttc acacatcatc
     3481 ggaaagtttc cggatgcctg agctgagcgt cgggggccgg gcaatcccca agacgcaacg
     3541 cctgaacggc gtttcgcttg gccttgcgcc gcgaccagcg ggacacgctg cgtcggcgat
     3601 tgcgagaaga gctcggccgc ccgctcctcg gcctcctcgt cgtcgccgaa gaggcttgcg
     3661 gccagcggtt ggcgacaact tggagccgtc gcgatagacg gccgtcgcct tcaggcccaa
     3721 gcgccaggac agcatgtagg cgtccttgca gtcctcgagg gtcactgcgt tcggcatgtt
     3781 gctcgtcttc gagatcgccc cgagataaag ggctgcaccg tcgccatcat gcggatgtgg
     3841 cggtcgacgg ataacgcgcc ggcgcggccg cacgggttgg tgcagtcgaa gagcctgatg
     3901 cttcggcttc aggtgcggcg ccggctccca ggtcatcgcg ccgcagcaga agctgttggc
     3961 ggcgtcgatc tcggcacggg cgaagccgag cgccgccaac aactcgaagg agcgatcacc
     4021 cagcgcgccc ggcgcgatcc ccagaatctc gatgggtcgg cgcagcagcc ccgacggccc
     4081 tgtcgatggc cgttcgtgtg gccattcgat ccagcccgcg aaggcactcg accgtgggcg
     4141 atcgagccga tccggtccgt cagcgcgccc gcgacaattt cgggttgatt tgttccgcgc
     4201 caggcgggtt ccttgctata ctcgcagcgg caggagctga acatggcggt tcaaccggtt
     4261 ggcgaaagct tgcggcgatc tgggcggccg acattgccga cacccgcgcg gcggccgagc
     4321 gcccggtcct cgattgttag agccgacttc gtctcgtctg accggatcgg tgcggcgggt
     4381 cggaggtcct cgcgcatcgc taaccgtcgg aacggctgat cctggcatca gacccgtccg
     4441 cggctcgcct gaccagtttg gcgcgggcgg gtcggcgtcg cgctgtcgtg atcgatcact
     4501 cagcccaagg accgcacagt gtcattctgc ggcgtccttg tttttcgagc catgcccatt
     4561 gtgtgagcct ctgggaacgg ccgctatcag cgctctgcag gcattctcaa gcggtagctg
     4621 gagggtctca aacgggtgga aagcggccat cccagctgtg cgaaaatcac aagacttgtg
     4681 gatgggtcgt tgcggtcatg ccgctagact gactctttgc tggtttagga gcgttcttga
     4741 ccatggccgc gcacgatgat catcgtaacg ggaacggatg ccaccacgtt ctactgattc
     4801 gggcgccccc ggaggcgata cgtttcgccg cgcttgctgc gatcgccatc ctggcattcc
     4861 tctggcttgg acgcgggctg ccgcgcatcc catgggcctc ctccgacagc gagagctatc
     4921 tccagtactc gccggtgcgt ccgcacggct actctctact cctctccggc tatcgcgtta
     4981 tcttcaccga tctcgcgcac ctcccgagcg tgcagctcgt cctttttgtc ggcgcggtgc
     5041 tgttgctcgc ggttgcggtc cagcggcgga cccgcaactt cgctgcagcg gtcgcgacgc
     5101 tcgtcgtcat ctttgcggcg accgacacaa ccagttttcc gtatgtcctc agcgatccca
     5161 tttatgcggc ggcgctgacg accggcatcg ccggtttcat cctctattcg gacacgcctc
     5221 gtcccggctt tctgctcctg gcgagcgcgg gcctcggcgt tgccgtcacc tttcgcgcca
     5281 tcggactggc actgctgccc ggtttttttc tcgcgattgt gctcgaccgc atcggccggc
     5341 gccgcggcct cgtcagcgcc gcggcggtcg gtacgttgcc catcgcagcg ctctgcctcg
     5401 ctgcagcctc gagccagctc atccacaacg ggcgcttcgc cctgggcagc tggggcggca
     5461 tggacgtgct cggaaaagtc ccgctactgg cgcgggctgt accggaggcg aatggacgcc
     5521 tggatgggtt cgtcaaggct atggagctag ggcgcagcaa gttgagacag ctcaatcctt
     5581 tcgtcgaggc cctcgcggcc agacaatatt atgagttcct cagatggtac gtgatcattc
     5641 ctgagctcga gcgctgctgg cccgactggc gtgacgccga cgactatcgc cggggggagc
     5701 tggcagccga attggcgaag gcctatatcg cacaagataa gctcggcttt gcgcgccgta
     5761 ctgcaatcga tctggccggc ctgtgggcga tgccgcgttg gctaaccgac agagagcggg
     5821 aggctgcgtt ggcggaaatc gaagccgtcg gcgaactgcc gggactgacc gcgttcttgc
     5881 gaacgccgga agcgcagctg gaatattaca agatcgttcc ctacgcgagc gatccgaccc
     5941 aaatcgcgat cttccgcctg accgtcgctt ccttttgggt cttgtcgctc ggcctcattg
     6001 caatcgttct gatcccgcgg cttagagctg caatgatgac gcccgatctc gtgctgatcc
     6061 tcgtgaccgt tcacgccatt tatatcggga cggcgctgat ggaatgcgca gagacgcgct
     6121 acatcatgcc gacgtggccg accctcgttg ccggtcccat tctcgctttc tggctgttcc
     6181 ggcggcttcg tagcgagacc ccctcacccg gctgtggttg gctgtgacag cggggtgaca
     6241 cctcccatgg cgtagggatt gcgccacggg ctcgctcctg ccgcgaactg agcggagaat
     6301 agcggcacca taaggggact gtctttgtcg cgttgtcgcg ggaagtcctg gacccgcaaa
     6361 ttggccggcc atgccggatc tttgcccttg ccggagatat ttagcaagct gaggggctgc
     6421 gaatctcgtc cggctgagga taaccctatt cagccgaacg caatgcgcgt cgggaccgac
     6481 ggcttggggg gccgtcagcg cgttgcgatt cccgacatgt gcgaccgctt agggcctgtc
     6541 aggaaattat cgaatcgact tctgagagag taaaatatgg gtttacaacc gtgtgatcat
     6601 accatatgtt gtggtggtga tcgatgttga acccatccgg gagcgttcca cggtagcgcc
     6661 gtttcttgac gagcggggcc gtcgtctggt cgcggcggcg gaagcgtttg ctgcgggtta
     6721 tggcggcatt accgcggtgg cgacggcaac cggcatggcg ccgagcagga tcgggcgggg
     6781 tctgggggaa ttggcccgca ggacgaacct tcggaacgtg ttcggcggcc gagggcggga
     6841 cgcaagccga cgatcctcaa ggacccgaca ttggttccgc atctcgaggc gctggtcgaa
     6901 ccgacgacgc ggggggatcc ggaggcgccg ttgcggtgga cctgcaagag cgtgcgacgc
     6961 ctcgccggag agctgcagca gcagggccac caggtcagcc gcaccctggt ttccgaattg
     7021 ctcaacgcgg cgggccacag cctgcagggc aaccgtaaga ccaaggaggg cgacagccac
     7081 cccgaccggg atgcgcaatt cgcccatatc aacacccaag tggccgcggc tttggccgaa
     7141 cagcagcctg tgatctcggt cgacaccaag aaaaaagagc ttgtcggcga cttccgcaac
     7201 aatggccgcg agtatcgccc gcaaggcagt ccggaagagg tccgggtgca cgacttcctg
     7261 atcaaggaac tcggccgggc ggcgccttac ggggtctatg acctggccgc caattgcgga
     7321 tgggtgagtg tcggcgtcga tcatgacacg gctgctttcg cggtcaatag catccgtcag
     7381 tggtggcgca acctcgggcg ggcgcgctac cccaatgcaa cgcgattgct gatcaccgct
     7441 gatggcggcg gcagcaacgg ctcgcaggtg cggttgtgga aacgcgaact ccagaagctg
     7501 gccaacgagc tcggcctcga catcgtggtc agccacctgc cccccgtacc agcaagtgga
     7561 acaaaatcga gcaccggctc ttctccttca tcagccagaa ctggcgcgcc aagccgctcg
     7621 tcagctatcg cgtgcgtgat tgtcgagctg atctcggcca ccaccacc
//
LOCUS       DATLJT010001290         2416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2171532,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001290 DATLJT010000000
VERSION     DATLJT010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171532"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VM910_29620"
     CDS             <1..417
                     /locus_tag="VM910_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HVI66702.1"
                     /translation="GIRQGTSNPVLTLPADVKDYPATVAQALSLLRLAGVNGPYALLL
                     GAEPYAAIGGAADDGYPVLRHVQHLMDGKIIWAPAIEGGVVLTTRGGDFDLTIGQDIS
                     IGYLSHSDTAVRLYLEETITFLLLTTEAAVSLAPKT"
     gene            430..624
                     /locus_tag="VM910_29625"
     CDS             430..624
                     /locus_tag="VM910_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66703.1"
                     /translation="MPDYDLYINPQKPSVGLYVRKGAGLPDLANPKEWVFDGTAAQGE
                     LPPDLVKKIEANGHAFRDMD"
     gene            complement(743..>2416)
                     /gene="sdhA"
                     /locus_tag="VM910_29630"
     CDS             complement(743..>2416)
                     /gene="sdhA"
                     /locus_tag="VM910_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HVI66704.1"
                     /translation="KVFPTRSHTVAAQGGMAASLGNMGDGDNWRFHMYDTVKGSDWLG
                     DQDAIEYMCREAVPAVLELEHYGVPFSRTEQGTIYQRPFGGQTIGYGKQMAQRTCAAA
                     DRTGHAILHTLYQQCLKHNTEFFVEYFALDLLMDDDGACCGLIAWNMEDGTLHRFRAH
                     CTVLATGGYGRVYFSCTAAHTCTGDGNAMVLRAGLPLEDMEFTQFHPSGIYGSGCLIT
                     EGARGEGGYFTNSQGERFMERYAPSAKDLAGRDVVCRAMTIEINEGRGCGPRKDYIEL
                     HLEHLGAALLHERLPGISETARIFAGVDVTREPIPVLPTVHYNMGGVPTNLHGEVVTK
                     RDGDPEAVVPGLMAIGEAACVSVHGANRLGSNSLLDIIVFGRAAAHRVMETLRPGDGR
                     APAPANATDRVIDRLDRIRWSKGGTGAGAIRLAMQRTMQRHCAVFRTGTLLQEGSVKL
                     DEVMKTMKSDLAVADRSMIFNTDLTEALELDNMLAQASVSLHSAIGRTESRGAHARED
                     FPKRDDDHWLRHTLSWLAEDGRVRLDYRPVHLQPLSNEVTTIPPKERVY"
BASE COUNT          444 a          764 c          748 g          460 t
ORIGIN      
        1 ggcatccgtc aaggcaccag caacccggta ttgacgcttc ctgccgatgt gaaggactat
       61 ccggctacgg tcgcgcaggc gctgagcctg ttgcgcctgg ccggtgtcaa tggcccctat
      121 gcgctgttgc tgggcgcaga gccgtatgcc gccatcggcg gagctgccga cgatggctat
      181 ccggtcttgc ggcatgtcca gcatctcatg gacggaaaaa tcatttgggc gccggccatc
      241 gagggcggcg ttgtgctcac aacccgcggc ggcgattttg atctcaccat cggtcaggac
      301 atctcgatcg gctatctaag ccattccgat acggccgtgc gactctacct tgaggaaacc
      361 atcacctttc tgctgttgac aaccgaggcc gccgtatcgc tcgcgccaaa gacgtgaagg
      421 agcatctcga tgccggacta cgatctctac atcaatcctc aaaagccctc agtcggtctc
      481 tatgtgcgca agggtgccgg tctccccgat ctggccaatc ccaaggagtg ggtgtttgac
      541 ggcactgcgg cacaaggcga attgccgccg gatctggtga agaagatcga ggccaacggt
      601 cacgcctttc gtgacatgga ttgagtgcgt tcggccgccg aaacgacgga gatgatatcg
      661 caaggagcgt cggggcaagg ttcgatgcca tcgcgatacg gactcaaatg gcgttctgat
      721 cgaatttacc ggacgatccc catcagtaca cgcgctcctt cggcgggatc gtcgtcacct
      781 cgtttgatag cggctgcaga tgaaccggcc gataatcgag ccggacgcga ccgtcctccg
      841 cgagccagga tagcgtgtgc ctgagccagt ggtcatcgtc gcgcttcggg aagtcttcgc
      901 gcgcgtgagc accgcggctt tcggttcggc caatcgcaga gtgcaggctg acgctcgctt
      961 gcgccagcat gttgtcgagc tcgagcgcct cggtgagatc ggtattgaaa atcatcgaac
     1021 gatcggcgac ggcgagatcg gatttcatcg tcttcatgac ctcgtcgagc ttgaccgagc
     1081 cttcctgcag cagcgttccc gtgcgaaaca ccgcgcagtg gcgctgcatg gtccgctgca
     1141 tggccaaacg gatggcgccg gcgcccgtgc cacccttcga ccacctgatc cgatcgagcc
     1201 ggtcgatgac acggtcggtt gcgttcgccg gcgccggagc gcggccgtcg ccggggcgca
     1261 aggtttccat gacccgatgg gccgcggcgc gaccgaacac gatgatgtcg agcaacgagt
     1321 tggatccgag gcggttcgca ccatgcaccg acacgcatgc cgcttcgccg atcgccatca
     1381 ggcccggcac gaccgcctcg ggatcgccgt cgcgcttggt gacgacctcc ccgtgcaggt
     1441 tggtgggcac gccgcccata ttgtaatgga ccgtcggcag caccgggatc ggttcgcgcg
     1501 tcacatccac gccagcgaag attctcgcgg tttcgctgat gccgggcagg cgttcatgca
     1561 aaagggcggc gccgagatgc tccaggtgca gctcgatata gtccttccgc gggccgcaac
     1621 cgcgtccttc gttgatttcg atcgtcatcg cgcggcagac gacgtcgcgg cccgccagat
     1681 ccttggcgct cggcgcgtag cgttccatga accgctcgcc ctgggaattg gtgaaatagc
     1741 cgccttcccc gcgcgcgcct tcggtaatca ggcagccgga gccgtagatg ccgctcgggt
     1801 gaaattgcgt gaactccata tcctcgagcg gcaggcccgc ccgcagcacc atggcattgc
     1861 cgtcgccggt gcaggtatgc gcagccgtgc aggagaaata gacgcggccg tagccgccgg
     1921 ttgccaacac cgtgcaatgc gcacggaagc gatgcagcgt tccgtcctcc atgttccagg
     1981 cgatgaggcc gcaacaggca ccgtcgtcat ccatcaatag atcaagcgca aaatattcga
     2041 caaagaactc cgtgttgtgt ttcaggcact gctgatacag cgtgtgcagg atcgcatggc
     2101 ccgtccggtc ggcggccgcg cacgtgcgct gggccatctg cttgccatag ccgatggttt
     2161 gaccgccaaa gggacgctga tagatcgtgc cttgctcggt gcgcgaaaag ggaacgccgt
     2221 agtgctcgag ctccagcacc gcgggcacgg cttcgcgaca catatactcg atcgcatcct
     2281 ggtcgccgag ccaatccgag cccttgaccg tgtcgtacat atggaagcgc caattgtcgc
     2341 catcgcccat gttgccgagc gaagccgcca tgccgccttg cgcggccacg gtgtggctgc
     2401 gcgttggaaa cacttt
//
LOCUS       DATLJT010001291         4223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_102848,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001291 DATLJT010000000
VERSION     DATLJT010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102848"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VM910_29635"
     CDS             <1..1357
                     /locus_tag="VM910_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66705.1"
                     /translation="VADAIARLIANGEDFELNRINALDFSNHNSLDEEKVISGFLHAS
                     RLGLFDLSWNVLCPGCGGVLGAHDTLKSLRPDDYHCGLCACGYEPSVDEQVEVAFTVS
                     PKIRRVAAHEPNTLPFWEYYRQVFWSSGIDLNKDNFASMKDEVALETLELPPDEKAVM
                     SLQLPPHFIIVFEPVTHSAQFIDVQGEPTKERQQLSLVYNKAHPPTGSMTLRPGPLRL
                     SLDNQSGLRVLPGIFVAGDKLHELLGKRRPFLTAKRMLSNQTFRDVFKADNLNVDQRL
                     KITSLTFLFTDLKGSTALYERVGDLAAFDLVRAHFRALLDIIASEKGAVVKTIGDAVM
                     ATFIRPENALMAGLKMRAAMGELNKQRGSKDLVVKIGIHEGPCLAVMLNERQDYFGQT
                     VNIAARVQSLSTSQEIHITAPVIESPGVVTILEKEEIRPIQKHAALRGIADKMVVYEI
                     P"
     gene            1552..2031
                     /locus_tag="VM910_29640"
     CDS             1552..2031
                     /locus_tag="VM910_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HVI66706.1"
                     /translation="MLDGLRQFIAEVVAPQNSQGRFFDDTGYRMAATALLIHIISLDG
                     EPSDREKRKLHSLVESQFGLDPGTADKLIASATLVEGEAVDLYHFTSVIMREVDEAGR
                     LRIVEMMWELVYADGQVSEFEDNVVWRAADLLAVSSRDRIELKHRVADKRSASVGGE"
     gene            2291..3070
                     /locus_tag="VM910_29645"
     CDS             2291..3070
                     /locus_tag="VM910_29645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66707.1"
                     /translation="MTERVTLITGASAGIGAELARVFARNGHRVALTARREDRLATLA
                     REITAAGGKSPILIPCDLEDHGARDKIASRLADADVDVEYLVNNAGFGLFGKAIELDH
                     EGQLGIIDVNIRALTDLSLRFSDQVIRNRGGILNVGSIAGFLPGPGMAVYYASKAYVL
                     SFTEALRKELEPHGVRVSVLCPGPVMTEFQARAGFAPGFDSTMLNVSAADVALQGYRG
                     LMANKRAVLPGFAIKMVPSMLRFAPRGFILAAVGRFQIRKP"
     gene            3205..4050
                     /locus_tag="VM910_29650"
     CDS             3205..4050
                     /locus_tag="VM910_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HVI66708.1"
                     /translation="MTLFDLTSHANVLPFPGRRTAAVSLCRESLAPVLIVLHQEKSTP
                     GRVGNVLRALGYRLDIRRPRFGDPLPETLDAHAGAIIFGGPMSANDPDDYVRQEIDWI
                     EIPLREQRPFLGICLGAQMLAMQLGAGVAPHPQGLTQIGYYPIRPTGAGVALCGAWPD
                     QVYHWHREGFELPRGAELLAEGDDFPVQALRFGHAFGFQFHPDVTTAMMHCWTARGAM
                     DLPGAQPRHRHFEGRAVYDVAERAWLTNFMSDWLAARADADGRGGGVILGKPVANQRF
                     SPFLT"
     gene            4108..4183
                     /locus_tag="VM910_29655"
     tRNA            4108..4183
                     /locus_tag="VM910_29655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4141..4143,aa:Ala,seq:cgc)
BASE COUNT          771 a         1277 c         1311 g          864 t
ORIGIN      
        1 ggtcgcggat gcgattgcgc ggctgatcgc gaatggagaa gatttcgaac tcaatcgcat
       61 caatgcgctg gatttttcca atcacaactc gctcgacgaa gagaaggtga tttccgggtt
      121 cttgcacgcc tcgcggcttg gcctgttcga tctgagctgg aacgtgctgt gccccggctg
      181 cggcggtgta ctcggtgccc acgacacgct gaaatcgctg cgccccgacg actatcattg
      241 cgggctttgt gcttgcggct atgagccctc cgtcgacgag caggtcgagg tcgccttcac
      301 cgtcagcccc aagatcagac gggttgccgc acacgagcca aacacgttgc cgttctggga
      361 atactaccgg caggttttct ggagctccgg gatcgacctc aacaaggaca actttgcctc
      421 gatgaaggac gaggtagccc tcgagacgct ggagctgcct cctgacgaga aggccgtgat
      481 gtcgctgcaa ctgccgccgc acttcatcat cgtgttcgag ccggtaacgc actccgccca
      541 gttcatcgac gtacagggcg agccgaccaa ggagcgtcag cagctttcgc tggtctacaa
      601 caaggcgcat ccgccgaccg gatcgatgac cctgcgtccg ggaccgttgc ggctgtcgct
      661 cgacaaccag tccggcctgc gcgtgctgcc cggcatcttc gttgccggcg acaagctgca
      721 tgagttgctc ggcaagcgcc ggccgttcct tacggcaaag cgcatgctgt ccaaccagac
      781 tttccgcgac gtcttcaagg ccgacaatct caatgtcgat cagcggctga agatcacctc
      841 gctgactttc ctgttcaccg acctgaaggg gtcgactgcg ctttatgagc gggtcgggga
      901 cctcgccgcg ttcgatctgg tgcgcgcgca tttccgcgcg ctgctcgaca tcatcgcgtc
      961 ggagaaaggt gccgtggtga agaccatcgg cgatgcggtg atggcgacgt tcattcgtcc
     1021 cgaaaacgcg ctgatggcgg gcctgaaaat gcgcgctgcg atgggtgagc tcaacaaaca
     1081 acgcggcagc aaggacctgg tcgtcaagat cggcatccat gaaggcccat gtcttgcggt
     1141 catgctcaac gaacggcagg attatttcgg ccagaccgtc aacatcgccg cgcgggtgca
     1201 gagcttgtcg acgtcgcagg agatccatat caccgcaccg gtgatcgaat cccccggggt
     1261 ggtcacgatc ctggagaaag aggagatcag gccgatccag aagcacgcgg cactgcgcgg
     1321 cattgccgac aaaatggtgg tgtacgaaat accttaggtg tcgttgcgcg aagcaatcca
     1381 gctcactgtc tccttctcgc aatgcgctgg atgtctggtt ccaaaaattg ccgaaacgta
     1441 atgtcgcatg cgaagttttt cgcattgtac gcgcgggctt ttcaactcat atacaaccaa
     1501 gtctcgcctc gcggcggaca atggaaacac aagtcattgc ggcggaaaaa catgctcgac
     1561 ggattgcgcc aattcattgc cgaagttgtt gctccccaaa actcccaggg tcgttttttc
     1621 gacgataccg gctatcgcat ggcggcgacg gcactcctga tccatatcat ctctctcgac
     1681 ggcgaaccgt cggaccgaga aaagcgcaag cttcacagcc tggtcgaaag ccagttcggg
     1741 ctcgacccgg gtaccgccga caaattgatt gcttccgcca ccctggtgga aggcgaggcg
     1801 gtcgatctct atcatttcac cagcgtgatc atgcgcgagg tcgacgaggc cgggcgcctg
     1861 cggatcgtcg agatgatgtg ggaattggtc tacgcggacg gccaggtcag cgagttcgag
     1921 gacaacgtcg tctggcgcgc agccgacctg ctcgcggtgt cttcgcgcga ccgcatcgaa
     1981 ctcaagcacc gcgtcgccga taagcggtcg gcatcggtcg ggggcgagta atggtcaccg
     2041 caagccgcgg cgaagcgtcg cgccgtactg cggctttttg gctcgtgccc gcatgcccgg
     2101 acatgaagaa actttaatgt agacgccgct tgccgccgac tgcggcaggt cagctaatcc
     2161 ccaacagact ttagcattct gcatttgcgc aaggctgcct gcttgcggga cggcttgcat
     2221 ccgtcctaaa gcctatgctc tcgtcggttt tcgacatcat agtctgcaat tgatttaaga
     2281 gccttccatc gtgaccgagc gtgtgacgct gattaccggt gcctcagcgg gaataggcgc
     2341 cgagcttgcg cgtgttttcg cgcggaacgg tcatcgtgtg gcattgacgg cgcggcgcga
     2401 agatcgcctg gcaaccctgg cacgcgagat cacggccgcc ggcgggaagt cgccgatcct
     2461 tattccctgc gatctcgaag accacggtgc gagagataaa atcgcaagta ggctggccga
     2521 tgcagacgtg gacgtcgagt atctcgtcaa caatgccggc ttcgggctgt tcggtaaggc
     2581 catcgagctc gatcacgagg gacaactcgg catcatcgac gtcaacattc gtgcattgac
     2641 cgacctatcg ctgcggttct ccgatcaagt gatccggaat cgcggcggta ttctcaatgt
     2701 cggttcgatt gccggcttcc tgccggggcc gggcatggcg gtgtactacg cgtcaaaggc
     2761 ctatgtgctg tccttcaccg aggcgttgcg gaaggagctt gagccgcacg gcgttcgggt
     2821 gtccgtgttg tgtcccggac ccgtgatgac cgagtttcag gcgcgggccg ggtttgcgcc
     2881 cggcttcgac tctacgatgc ttaacgtatc ggccgccgac gtcgcgctgc aaggctatcg
     2941 ggggctgatg gccaacaagc gggccgtgct gcccgggttc gccatcaaga tggtgccttc
     3001 catgctgcgg tttgctccgc gcggtttcat tctggctgcg gtcggccgct ttcagatccg
     3061 caagccgtaa gccgcgacag aaggttcgcg gccttgcgcc tccttcttgg cccgctcttt
     3121 gcttggtttc gcaccgtgcc gcaactcaca ggatattaac gcttgcttag ctaacatttc
     3181 cgctcgtgtg cgcatgcgga gccaatgacc ttgtttgacc tgaccagcca cgcgaatgtg
     3241 ctgcccttcc cggggcggcg aacggctgcc gtttcattgt gcagggaaag cctggcgccg
     3301 gtgctgatcg tcctgcacca ggagaaatcg acgcccgggc gggtcggtaa cgtgttgcgg
     3361 gcgctcggct accgcctcga catccgccgt ccccggttcg gcgatccgct gcctgagacg
     3421 ctcgacgcgc atgcgggtgc gatcatcttt ggcggcccga tgagcgccaa tgatcccgac
     3481 gactatgtgc gacaggagat cgactggatc gagattccgc tgcgcgagca gcggccgttt
     3541 ttaggcattt gcctgggcgc gcaaatgctc gcgatgcagc tcggcgccgg cgtcgcgccg
     3601 catccgcaag gcctgaccca gatcggctat tacccgatac gcccgacggg cgcaggcgtg
     3661 gcgctgtgcg gcgcctggcc cgatcaagtc tatcactggc accgcgaagg ctttgagttg
     3721 ccgcgcggcg ccgaactgct cgccgagggc gacgattttc ccgtgcaggc actacggttc
     3781 ggccacgctt tcggcttcca gtttcatccc gacgtgacga ctgcgatgat gcattgctgg
     3841 accgcgcgcg gcgcgatgga tttacccggc gcgcagccgc gtcatcgtca cttcgaaggg
     3901 cgtgcggtct acgacgtggc ggaacgcgcc tggctgacga atttcatgag tgactggctg
     3961 gccgcgcgag ccgatgccga tggcagaggc ggcggagtaa tccttggcaa accggtggca
     4021 aatcagcggt tttcgccgtt cttgacttga ctgtggtgcg gatttaaatg gcccagattg
     4081 cgagccgcct tacaaggcgg cgggtacggg gccatagctc agctgggaga gcgcatcgtt
     4141 cgcaatgatg aggtcggcgg ttcgatcccg cctggctcca ccagccttgg ctcgcgtcgc
     4201 gagcctccgg ctggcaggtc acg
//
LOCUS       DATLJT010001292         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2401488,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001292 DATLJT010000000
VERSION     DATLJT010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2401488"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..586)
                     /locus_tag="VM910_29660"
     CDS             complement(164..586)
                     /locus_tag="VM910_29660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66709.1"
                     /translation="MKNRFLVSLALAVVVAAPATAAELPLEAPPPLLAAYYDWSGAYV
                     GFNVGGTHYNVTHHFPSPGAITPDVTTGDHDAIFGFHAGAQSQWGAWVLGAEVARSGC
                     NHECRSTSSVLPVSQGFEENIFGQHKIIDLLTAGARVG"
     gene            complement(752..1051)
                     /locus_tag="VM910_29665"
     CDS             complement(752..1051)
                     /locus_tag="VM910_29665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66710.1"
                     /translation="MPYHSSRVRDWNENDVIAIALTTASISVAAVLDDLKRRLPRALD
                     PLGGDAMTTKEAFEIVLALAFDHMVDEDEMPEIYKQQSEALEIVTEIAQYYGSRR"
     gene            complement(1141..1347)
                     /locus_tag="VM910_29670"
     CDS             complement(1141..1347)
                     /locus_tag="VM910_29670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66711.1"
                     /translation="MTLQEAKSIARHLGLTLRKVRSGDYRVSCRDGDETTAYYTQNLE
                     DAVNAAVEIARKRAPRAEPYGVGR"
     gene            complement(1568..1795)
                     /locus_tag="VM910_29675"
     CDS             complement(1568..1795)
                     /locus_tag="VM910_29675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66712.1"
                     /translation="MKVLVVIGAIALLPMLALAQQSQLPQPRKPGEWCPEGWSANGNY
                     CVAGSDKARPAILKNGWCPVGWRESGSYCIR"
     gene            complement(1809..2234)
                     /locus_tag="VM910_29680"
     CDS             complement(1809..2234)
                     /locus_tag="VM910_29680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66713.1"
                     /translation="MPGQAIAATAPTNKSLAQRSKSLDTGKATKTLPRRTDPARPIAV
                     QVRSFDVRGSLGRDCGINRPMERNPMSLHFRRSAGLGRNAHVQQAAVVAQPTAIEERF
                     WQAVALEIAEGLCKAVLLIGSIVAGIVLGVVVAAMSSQL"
     gene            2241..3305
                     /locus_tag="VM910_29685"
     CDS             2241..3305
                     /locus_tag="VM910_29685"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI66714.1"
                     /translation="MGIGTSSALNSEQDLASIPMAGGGLSRLAIARLKNAGVDVAPLL
                     KRAGLTPEAIAEPGERLSVRSQIALLDEAAIALKDDCIGFTLARDFDPREIGLLYYVM
                     ASSQTLGDALKRIARYSKITNEALVFGYQEGNRLVLSLSYSGVSRHSDRHQIEFCMFG
                     GLRLCRMLTGQNLVPQHFSISHHRSQGTAEMARFVGTKVEFGADRDEFALNVDARELP
                     FIHADSYLNDLLLKYCEAALADRRGDMSQLRTRVENAISSVLPHGKVRVDDVARSLGM
                     SKRTLARRLSDEGLNFTEILQQLRRDLAVRYLDDRKLHVSKIAWLLGFHEVSAFTHAF
                     KRWTGKTPREIRTAGVTMTA"
BASE COUNT          631 a         1025 c         1021 g          679 t
ORIGIN      
        1 gtagtcgaag ccgccgcccg cataccagcc gcggttgttg ctcacgacgc cgtttccagg
       61 gccgggcgcg tcgcaaatac cggtgatggt cgagcaatgg gcgttctcga tatgggccga
      121 ggcaaagccc ccgttcgcga agaccagcaa ccgatcccag gcattagccc acgcgcgcgc
      181 ccgccgtgag cagatcgatg atcttgtgct ggccgaagat attttcttcg aagccctgag
      241 agaccggtag gacgctgctc gtgctgcggc attcgtggtt gcatccgctg cgcgcgactt
      301 cggcgccgag aacccacgcg ccccactgcg attgcgcgcc ggcgtggaag ccgaagatgg
      361 cgtcgtggtc gccggtggtg acgtcgggag tgatcgcgcc gggcgatggg aaatggtgcg
      421 tgacgttata gtgcgtcccg cccacgttga agccgacgta ggcgccggac caatcgtagt
      481 acgccgctag aagcggaggc ggcgcctcga gtggcaactc cgccgccgtc gcaggcgctg
      541 cgaccaccac ggcaagcgct aggctgacaa gaaagcgatt cttcatgacg gccgccccaa
      601 gccgactgtc gacggttgag catagcgctt cccggcggct tcggctgtga cctttcggca
      661 acgcgcaacg tcattcgagc gtccggcgcg cggcgcggcg cggcggatga attctgctgc
      721 tcacgcgcca atcgggccgg ggccggcgtc atcaccgcct ggacccgtag tattgggcta
      781 tttccgtgac gatctcgagc gcttcggatt gctgcttgta tatctcgggc atttcgtctt
      841 catcgaccat gtggtcgaag gccaacgcca gcacgatctc aaaagcctct ttcgtcgtca
      901 tggcgtcacc tcccaatggg tccagcgcac gcgggaggcg ccgtttgaga tcatccagaa
      961 cggcggcaac cgagatgctt gccgtcgtga gcgcgattgc aatgacatca ttctcattcc
     1021 agtctcttac gcgggagctg tggtacggca tggctttgca ccgcctgcct cgttcggcgt
     1081 tcggttgttt ccgttcgaaa gcgcgcactc gagcatcgag aacagcttgg ttgccggatt
     1141 tcacctgcca actccgtacg gttcagctcg cggcgctcgc ttacgggcga tctccaccgc
     1201 ggcattgacg gcgtcttcga gattctgcgt gtagtaggcc gtggtctcgt ctccgtcgcg
     1261 gcagctcacg cgatagtcgc cggagcgcac cttgcgcagg gtgagcccga gatgtcgcgc
     1321 gatcgatttg gcctcctgta gcgtcatggc gcatcaatac cctgtcgata cccgcgcaaa
     1381 tggcggttcc acgtcttagt cgctttgccc cccgtgctgg acttgtcact ttgcgccaga
     1441 catttgttcg ttggagccag aagcggtggc gtgggcggca atcattacgc cgcgggccgc
     1501 gcgcggctgg ccggcgcgcc atcatttgac agtgccgacg atgcaagatg cccgctgctg
     1561 tggaccctca gcgaatgcaa taactgccac tctcgcgcca cccgaccgga caccaaccgt
     1621 tcttgagaat tgccggccgc gccttgtcgg agcccgctac acaatagttg ccgttcgcgc
     1681 tccagccttc cgggcaccat tcgcccggct tgcgcggttg cggcaattgc gattgctgcg
     1741 cgagcgcgag catcggcagc agcgcaatcg caccgataac gaccagtact ttcatgacat
     1801 ctccttcgct aaagctgtga cgacatcgcc gccacaacga cgcccaaaac gatcccagcg
     1861 acgatcgagc caatcagcag aacagctttg caaagcccct cggcgatctc caaggcaacg
     1921 gcttgccaga atcgctcctc gatcgcagtc ggctgcgcga cgacggcggc ttgctggaca
     1981 tgcgcgttcc tgcctaaccc tgctgaacgt ctgaaatgaa gcgacatcgg attcctttcc
     2041 attggtcggt tgatgccgca atcgcgtcca agcgagccgc gcacgtcgaa gctgcggacc
     2101 tgaacagcaa tgggccgtgc cggatcagtt ctcctcggca acgtcttagt cgctttgccc
     2161 gtgtccagag acttgctacg ctgcgccaaa cttttgtttg tcggagccgt ggcggcaatt
     2221 gcctgcccgg gcactcaagg atgggcatcg gcacctcttc agccctgaat tccgaacaag
     2281 acttggcgtc gatcccgatg gcgggaggcg gcctcagcag gctggcgatc gcacgcctca
     2341 aaaacgctgg cgtggacgtg gcgcctcttc ttaagcgtgc gggactgacg ccggaggcaa
     2401 tcgccgaacc gggagagcgg ctgagtgtgc gaagccaaat cgcgcttttg gacgaggcgg
     2461 cgattgctct gaaggacgac tgtattggct tcacgctggc tcgcgatttc gatccgcgcg
     2521 aaatcgggct gctttattac gtcatggcgt cttcgcagac cttaggcgat gcactcaagc
     2581 gcattgctcg ctacagcaag attaccaacg aggccctggt gttcggatat caagagggaa
     2641 acaggctcgt tctcagtttg agttactccg gcgtttcacg gcattcggac aggcatcaaa
     2701 ttgaattttg catgtttggt ggattacgac tttgccgcat gttgacgggg cagaacctcg
     2761 taccgcaaca tttctcgatt tcccaccatc gttcgcaagg cactgccgaa atggcgcgat
     2821 tcgtcggaac gaaagtggag ttcggcgccg acagagacga gtttgcccta aacgttgacg
     2881 cgcgtgagtt gccgtttatt cacgccgaca gctatctcaa cgaccttctc ctgaaatatt
     2941 gcgaagcggc gctagccgat cgaagaggcg acatgagcca gttgcgtacg agagttgaga
     3001 acgcgatctc gtcggtgctt ccgcatggaa aggtgcgcgt ggacgacgtt gcccgcagtc
     3061 tgggaatgag caagaggacg ctggcacgaa ggctctccga cgaagggttg aacttcactg
     3121 aaatacttca gcaactgcgc cgcgatctgg cagttcgtta cctcgatgat cgcaagctgc
     3181 acgtatccaa gatcgcgtgg ctgctcggtt ttcatgaggt gagcgcgttc acgcatgcgt
     3241 tcaagcgctg gaccggaaag acgccgcgcg agatccggac tgccggcgtt accatgacag
     3301 cgtgagccag ccgacctata ccttcaaggg ggcgatgccg aaggccggcg acgtct
//
LOCUS       DATLJT010001293         1542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_565108,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001293 DATLJT010000000
VERSION     DATLJT010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565108"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1168
                     /locus_tag="VM910_29690"
     CDS             350..1168
                     /locus_tag="VM910_29690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI66715.1"
                     /translation="MSASGSSLAEFCRLHVPALEQNEVRHNVMLAVLGGAIDRQLADV
                     VTWSLGSPGQCATMNPGRPILLADVKEAQCRSLAEQTTQLDYAGVVGPDQTALWFARR
                     AAELGVKFLGPIPQRIHSLSDKPRYPGCPGHPRPVNADDADLLADWIAAFIGEATPHD
                     PTPARDELRRVAAQGRHVFWIVQEQPVSMAAIVRRTRNAAAIALVYTPPSLRGKGYGG
                     SVTAAVVEQAFAEGKTVACLYTDLRNPYSNRCYVKIGFRPECDSFHIPRDIAAC"
     gene            1309..1515
                     /locus_tag="VM910_29695"
     CDS             1309..1515
                     /locus_tag="VM910_29695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66716.1"
                     /translation="MNKREFITLLGGAAWPLTARAQQAERMQRIGVVRIASRLGLERR
                     AREIEEPIDQFRTYFIIGCATVAG"
BASE COUNT          307 a          477 c          474 g          284 t
ORIGIN      
        1 tggtagaacc ggatagcgag gaaccggtgt ccaaatggcg agatgccatc aacaagacgc
       61 aggcttggct tgacgccatg cggtaggcca taccgacatt tccgaacaat taatcctcgc
      121 gctgggcttc gttactcata aatcgcccag ccgtgtccgc tttcgggcaa accggacatt
      181 tgagcggaca tcgcagaagg gccgagtttg acccgcagcc gacatcggcc aaattgaaat
      241 cccgcagcgc agcagtgcgt gttatccttt cgggacctac caaatggcgg caggccgaag
      301 aagcacctca tcgaatcgga tgtcagcggg acaacgctgg cttgaacaga tgtcggcatc
      361 aggctcatcc ctcgcggaat tttgtcgcct gcatgtgccc gcactcgagc aaaacgaagt
      421 gcgccataac gtgatgttgg ctgtactcgg cggagcgatc gatcgccaac ttgcggatgt
      481 agtgacctgg tccctcggtt cgccgggtca atgcgcgacc atgaaccccg gcaggccgat
      541 cctgcttgcc gacgtcaagg aagcgcagtg ccgatcgctc gcggaacaaa ccacgcagct
      601 cgattatgcc ggcgtcgtcg gtccggatca gaccgcgctt tggtttgcta ggcgcgcggc
      661 cgaactcggc gtgaaatttc ttgggccgat cccgcagcgc atccacagcc tcagcgacaa
      721 accaagatac ccaggctgcc cgggacaccc gcggcctgtg aacgccgatg acgccgatct
      781 gctggcggac tggatcgcgg cttttatcgg cgaggcaacg ccgcacgacc cgacgcctgc
      841 acgcgacgag ctgcgcaggg tcgcagcgca aggccggcat gttttctgga ttgtgcagga
      901 gcagcccgta tcgatggcgg cgattgtgcg ccggacccgc aacgccgccg ccattgcatt
      961 ggtctacact ccgccctcac tgcggggcaa aggttacggt ggatccgtga cagcggcggt
     1021 cgtggagcag gcatttgccg aaggtaagac cgtggcgtgc ctctacaccg atctgcggaa
     1081 cccatattca aaccgttgct atgtcaagat tggcttcagg cccgagtgcg actcatttca
     1141 catcccgcgc gacatcgcgg cgtgctgacc cttcgggccg ctcgtagggg cgacctgcta
     1201 attttggtct gatacgtaga gaagggcctt cgttagcccc aagaccgcca ggacgcttgg
     1261 cctcgaaatc ccgccgatgc tgctcgcccg cgccgacgag gtgatcgaat gaacaaacgc
     1321 gagttcatca cgctgctcgg cggcgcagcg tggccgctga cggcgcgcgc gcagcaggcc
     1381 gagcggatgc agcggatcgg cgtggttcgc attgcatcgc gtctcggcct tgagcgtcga
     1441 gcacgcgaga tcgaggagcc aatcgaccag tttcgcacct atttcataat cgggtgcgct
     1501 accgtcgccg ggtagatcgg gcagatagcg cccggtctat ca
//
LOCUS       DATLJT010001294        10712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1942864,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001294 DATLJT010000000
VERSION     DATLJT010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942864"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VM910_29700"
     CDS             complement(<1..615)
                     /locus_tag="VM910_29700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI66717.1"
                     /translation="MPRALFPALFCGNNVASEESREFAQLATTISFGTGKTIFAEGEP
                     ADSAFGVSQGVVRLYKLLPDGRRQVVAFALPGDFLGMPLATRHSCYADAIGAVSLCRF
                     SRTELSSLIQSSPDIERRLIEFIARELDRARDQLLLLGNASAEERVAIFLVNWRNRLE
                     RVGPVSQPLPLPMPRQDIADFLGLKLETVSRTLAKLEQKNVIRLV"
     gene            880..1089
                     /locus_tag="VM910_29705"
     CDS             880..1089
                     /locus_tag="VM910_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66718.1"
                     /translation="MTASKMVSAPEALPRKKPAANCGPMAQQGAMGAHGHQMHPQEFV
                     RLIGCRDTKKQEDEVPEREVPERVH"
     gene            complement(1106..1468)
                     /locus_tag="VM910_29710"
     CDS             complement(1106..1468)
                     /locus_tag="VM910_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66719.1"
                     /translation="MSDIGKKNHTVRPGRDAISAVQLSQSLTAEIDAWAEAHHISRSD
                     AIGRLVELGLKASRAVSSQHSLQQDSLNIEDEAIRLIRSLLDPSLPDEERERRIRRLV
                     EGPPEFSEQRIDLPKHQK"
     gene            1636..2352
                     /locus_tag="VM910_29715"
     CDS             1636..2352
                     /locus_tag="VM910_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVI66720.1"
                     /translation="MQFVKTFQLYDFLDTLISLLVAFVFGTLIGAERQYRQRSAGLRT
                     NVLVAVGAAAFVDLADHLNGAEGAVRVVAYVVSGIGFLGAGAIMKEGMNVRGLNTAAT
                     LWASAAVGSCAGADMVAQSAALTLFVLAGNTLLRPIVNAIDRIPLSEHSSEAVYEISV
                     TTDAKRAAEVRDILNERLEAASYPVRESKIVFRSNDNVEVAAILVPLAVEPGELDAVV
                     SDLSKLPGVSHATWSMSALE"
     gene            2442..4073
                     /locus_tag="VM910_29720"
     CDS             2442..4073
                     /locus_tag="VM910_29720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66721.1"
                     /translation="MATRREFVLGAAAATLPHLITTGIGGPASAAEGPPPREKDWDQG
                     DVQHLIPTASHDRFLIKVSFTQAQEAAPELEVGPTRVRGQPNAAAGDFWQFDVTGLSP
                     ATTYQLSLVSANGRALCAPWSLSTFPAPDATPDRLRLMIYTCGGGHDALNQGLPEGKT
                     TWLPSALRRRLLQRGLSFKPDALIANGDQIYWDLRAPQASKLIGERGAQIAGTFDRAQ
                     PVFGTANEAVFRRATGPQIIPQYGALLRSTPVFFMQDDHDYFDNDEATDEIITFPPDH
                     FMLELARATRQLYYPEYLPDPNRPLGLPGASAADRPPGVAEAFGTLRYGKLAEILLYD
                     IRRTQTLAGPSAVFVDGTVEAWLKSRMADREVIHVVNIPSNPPGWSAGKWGEWYPDIL
                     GKDGKLTTDVAKPYWQSGWLKQHDRLMAAIAAMEGRIPLVVSGDLHAIAEGRMMQCGA
                     LDFAKKPVNVILSGPLGTGDRGWPSAFRGIGASPPNHLTMEEDLKPIEENGFILADFS
                     PENITVRYFRFNYHKQPGEVIDTLEPFRVTELKRS"
     gene            complement(4111..4911)
                     /locus_tag="VM910_29725"
     CDS             complement(4111..4911)
                     /locus_tag="VM910_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HVI66722.1"
                     /translation="MTGPDPTSQQITAHALLIGDRINTAGFEGQVLSNTPLAVRIGRN
                     GLAVVFRYGVAVFLGLSADEEAEFLERLHPRTFGKITPYEEEFAKVQVARESDEPIPP
                     GGPIPVREMSLERLLVVADALAKSVVLARDERQVANVVDTVEPFARELARAGRTSRNR
                     TDLLKLLGNALLVQHRLSGRVAVGEKPDVLWDRPDLERLYARLEDEYELSERVETLNR
                     KLAVISDTATTLADIIDAKRTLRLELVVIFLIAFEIVVTAYEIYGRGH"
     gene            5083..6237
                     /locus_tag="VM910_29730"
     CDS             5083..6237
                     /locus_tag="VM910_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HVI66723.1"
                     /translation="MTTRGGSDTFYAGIPVFRGFTRLMEPSLYSPLPGDWTVGVADIV
                     ESTKAIAAKRYKAVNMAGAAVIAAVTNALEGREFPFVFGGDGASFAVAPEDLAAAREA
                     LAATAAWVKADLDLVMRVALVPVAAIRAQGADVRVARFGPSANLSYAMFSGGGLGWAE
                     AAMKRGEFMVAEAAVGTQPDLSGLSCRFEEIPATRGLILSVLVLPTQGADLPAFRKVI
                     EDIVGLVEKSPDSGRPVPPGGPPLRWPPQGVEYEARAARGGSLAKRRVVVLAVTLWAY
                     VVMRFGIKVGKFVPKTYLQQVVENSDFRKYDDGLRMILDCNEELAHALTERIAAAAAS
                     GTVRYGLHRQDAAMMTCFTPSVMRADHVHFIDGARGGYASAAIALKAMTA"
     gene            6409..7008
                     /locus_tag="VM910_29735"
     CDS             6409..7008
                     /locus_tag="VM910_29735"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HVI66724.1"
                     /translation="MTFTLPPLPYAYDALGPYMSKETLEFHHDKHHQAYVTNGNNAIK
                     GTEFEGKSLEEIVKGSFGKNAAVFNNAGQHYNHIHFWGWMKPKGGGSKLPGSLEKKIN
                     EDLGGFEKFKTDFIAAGVGQFGSGWCWLQVKNGKLEIAKTANGENPLVHGATPILGCD
                     VWEHSYYIDYRNRRPDYLKAFVENLVNWDYVEQLFAKTS"
     gene            7366..7950
                     /locus_tag="VM910_29740"
     CDS             7366..7950
                     /locus_tag="VM910_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HVI66725.1"
                     /translation="MRPTNDAEGEPRPARARKPVGWSMITIAVLVAVSAALVWRRDGL
                     PGVLDILTHDVSLFGGILPRVLAGCLLGAFIAEILPHDKVSRSLGPKSGVKGLLIGTA
                     FGAILPGGPFTAYPVASALLAVGADFGATIAMVVSWTLIGYGRAVAWELPIMGTDFTL
                     WRIVISLPLPVLAGALGRFVYARMYPAKGTDDSE"
     gene            7947..8447
                     /locus_tag="VM910_29745"
     CDS             7947..8447
                     /locus_tag="VM910_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66726.1"
                     /translation="MSAALLIDIMLWGSVAAVGLIAYRRGPTVLNASLREGAMDFINI
                     IPRIALGVIGSGYIAAVIPPEVITTWLGPNSGWFGVAAAVLAGAATPGGPVVGFSIGA
                     VALKSGGGAPQVLAYVIAWALFAFQRLLLWEIPFMPARFVWFRAAVSVPFPFLAAAIA
                     MAIGKP"
     gene            8718..9473
                     /locus_tag="VM910_29750"
     CDS             8718..9473
                     /locus_tag="VM910_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66727.1"
                     /translation="MAPKLIFQNVTLGYDRHPAVHHLSGEVVSGALLAVMGPNGAGKS
                     TLLRGIAGILKPLSGSISLGSIDTLDIAYLPQTAEIDRSFPVSVFDFVGTGLWRRVGF
                     FGGIGKEQRNQIESALGAVGLTGFENRPIGTLSGGQMQRLLFARVLLQDARLIVLDEP
                     FNAIDAKTCADLLALVKHWHGEGRTVLAALHDLEMVRAHFPDTLLLARGPVAWGPTAH
                     VLTADNLLVAMRMCEAFDDAAAACAVDTPSRAA"
     gene            9479..10348
                     /locus_tag="VM910_29755"
     CDS             9479..10348
                     /locus_tag="VM910_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HVI66728.1"
                     /translation="MLADALIAPFTEFEFMRRALAAIVALSLGGAPIGVFLLLRRMSL
                     VGDAMAHAILPGAAIGFLLSGLNLFAMTTGGLIAGFAVAILAGVVARSTGLKEDASLA
                     TFYLASLALGVTIVSIKGTNIDLLHVLFGDILAMDDQTLLLVAINATITLLVLAVIYR
                     PLVIECVDPLFLRTVSRAGAPAHLAFLALVVINLVSGFQALGTLLAVGLMILPAGIAR
                     FWSRDVTPMICIAVLSAIVSGYAGLVLSFQTRIPSGPAIILVAAALYIASILFGSVGG
                     LVRQLFPRRHLEA"
     gene            10353..>10712
                     /locus_tag="VM910_29760"
     CDS             10353..>10712
                     /locus_tag="VM910_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HVI66729.1"
                     /translation="MRRLMVWSICLAAIVCPARAQDRLNVVASFSILADFVKNVGGDN
                     VDVTTLVGPNGDVHVYAPAPSDAKKIADAKLLVINGLGLEGWLPRLLQSSGSKAPIVV
                     ATRGITPLKLGSDADPHA"
BASE COUNT         1811 a         3400 c         3395 g         2106 t
ORIGIN      
        1 aacgagtcga atgacgttct tctgctcgag cttggcgagc gtgcgactga ccgtttcgag
       61 ctttaagccc agaaagtcgg caatgtcctg gcgtggcatg ggaagcggca gaggttgcga
      121 aaccgggccg acgcgctcca gccggttgcg ccagttcacc agaaagatcg ccaccctttc
      181 ctccgccgag gcgttgccga gtagcagcaa ctgatcacgc gctctgtcga gctcgcgtgc
      241 gatgaactcg atcaaccgcc gttcgatgtc ggggctgctt tgaatcaggc tcgaaagctc
      301 cgtgcgcgaa aaccggcaaa gagaaactgc gccgattgca tcggcatagc agctatgtcg
      361 cgtcgcaagc ggcattccga gaaaatcgcc tggcaacgcg aacgccacaa cctgacgccg
      421 tccgtccggc aacagcttgt agagccgcac gacgccctgt gaaactccga acgcgctgtc
      481 ggccggctcg ccttcggcaa agatggtctt tccggtgcca aacgaaatcg tcgtggcgag
      541 ctgcgcgaac tcgcgagact cctcactcgc tacgttgttt ccgcaaaaca gtgcggggaa
      601 gagagcacga ggcatgacat ccacttgtgc ccagtacctc gacgccgccg atgcattcat
      661 tgcaccgagt caactcgacg ttgacccata ccgggcaccg gtatgattag gagccctgcg
      721 aaatagcgtg ttgatacagc tcaaccacgc gacggaacgc gactctgagt tgattcgaat
      781 caattcgtgc ccgacgcgga taggtgatat gataagcgag atcgattggt tcccactagg
      841 tcaatcgatc tgcccttttc ttcaaaaagg gagctgtcca tgaccgcttc gaaaatggtc
      901 tcggcgcctg aagcgctgcc gcggaaaaaa cctgccgcca attgcggccc gatggcgcaa
      961 cagggggcca tgggggcgca tggtcatcag atgcatccgc aggaatttgt gcggctgatc
     1021 ggctgcaggg ataccaagaa gcaagaagat gaagtgcccg agcgcgaagt gcccgagcgc
     1081 gtgcattagt acatggcggt ctgcatcact tctggtgttt aggtaaatcg atacgctgct
     1141 ccgagaactc cggcggtcct tcgacgagcc tccggatacg acgctcgcgc tcctcgtcag
     1201 gaagcgacgg atcgagcaga ctgcggatca gcctgatcgc ctcgtcctcg atgttgagtg
     1261 agtcttgctg gagggagtgc tgcgaactca ctgcccgcga cgccttcaga ccgagttcca
     1321 cgagccggcc gatcgcgtcc gagcggctga tgtggtgcgc ttccgcccag gcgtcgatct
     1381 ccgcggtgag gctttgcgac agttgaacag cgctgatggc atcgcgaccc gggcgaaccg
     1441 tgtggttttt cttgccgata tcagacacac ttttgactct accctcgaca cctctcgatc
     1501 ttgctctcta taggcttttt caccagcccg gatttgattg agatcaatga gggcgcgccg
     1561 ctttcggtcc tgcggctgcc ttgttcggcc atgctatttt gcgttagatg ccgcggcccg
     1621 accgaggtgc gccccgtgca gttcgtcaag acctttcagc tttatgattt tctcgacacc
     1681 ctgatcagcc tgttggtcgc gttcgtgttc ggcaccctga tcggggccga gcgccaatac
     1741 cggcagcgca gcgcgggcct gcgcaccaac gtcctcgtcg cggtcggcgc ggccgccttt
     1801 gtcgatcttg ccgatcacct gaacggggcc gagggcgcgg tgcgggtggt cgcctatgtg
     1861 gtctccggca tcggattttt gggcgccgga gcgatcatga aagagggcat gaatgtccgc
     1921 gggctcaata cggcagcaac attgtgggca tccgctgcgg tcggctcctg tgccggcgca
     1981 gacatggtgg cgcaatcggc ggcgctcacg ctcttcgtgc tggcaggcaa cacgctgctc
     2041 cgtccgatcg tcaatgcgat cgaccgcatc ccgctgagcg agcattcgtc cgaagcggtc
     2101 tacgagatca gcgtcacgac cgacgcgaag cgtgcggccg aggtgcgcga tatcctgaac
     2161 gaacgtctcg aggccgcgag ctatccggtg cgggagagca agatcgtgtt ccggtcgaac
     2221 gacaatgtgg aggtcgccgc cattttggtg ccgctcgcgg tcgaacctgg cgaactcgac
     2281 gccgttgtca gcgatctatc gaagttgccc ggggtcagtc atgccacctg gagcatgagc
     2341 gcgctggagt agagcttcga ccttgttcga tcggcaggcc gggcgcaata tccgaagaaa
     2401 acgcgcgcag cggaaggacg tgaacaacaa ggagagcaca gatggcgacg cgacgcgaat
     2461 tcgtgcttgg cgcagccgcg gcaacgcttc cacatttgat cacgacgggt atcggcgggc
     2521 cggcatcggc agccgaggga ccgccgccgc gcgagaagga ctgggatcag ggtgatgttc
     2581 aacacctgat cccgaccgca agtcacgacc gctttctgat caaggtgtcg ttcacccagg
     2641 cacaagaggc tgcacccgag ctcgaggtcg gtcccacacg cgtgcgcggc cagccgaatg
     2701 cggccgccgg cgatttctgg cagttcgacg tgacggggct ttcgccggca acgacctacc
     2761 agctctcgct tgtttccgcg aacgggcgcg cgctttgcgc gccctggtcg ctctcgacat
     2821 tccccgcgcc ggatgcgacg ccggaccggc tgcgcctgat gatttacacc tgcggcggtg
     2881 ggcatgatgc gctcaaccag ggactgcccg agggcaagac gacttggctt ccatcggcgc
     2941 tgcgtagacg gcttctgcag cgcggtctct ctttcaagcc cgatgcgctg atcgccaatg
     3001 gagaccagat ctattgggat ctgcgcgcgc ctcaggcgtc caaattgatc ggcgagcggg
     3061 gcgcccagat cgcgggcacc tttgatcgcg cgcagccggt gttcggtacg gcaaacgagg
     3121 cggttttccg ccgcgcgacc ggaccccaga tcattccgca atatggtgcg cttttgcgtt
     3181 cgacgcccgt cttcttcatg caggacgacc acgactattt cgacaatgac gaagcgaccg
     3241 acgagatcat tacctttccg cccgatcact tcatgctgga attggcgcgc gccacgcgcc
     3301 agctctatta tccggaatat ctgccggatc cgaaccgccc actcggtctt cccggtgcct
     3361 ccgccgccga ccggccgcct ggcgtcgccg aagccttcgg cacactgcgc tacggcaagc
     3421 tcgccgagat cctgctgtac gatatccgtc gcacgcagac gctcgccgga ccgagcgcgg
     3481 ttttcgtcga cggcacggtc gaagcctggc tgaagtcgcg gatggccgac cgcgaggtga
     3541 tccacgtcgt caacattccg tccaatccgc ccggatggag cgccggcaag tggggcgaat
     3601 ggtatcccga cattttgggc aaggacggca aactgaccac cgacgtggcg aagccttatt
     3661 ggcagagcgg ctggctcaag caacacgacc ggctgatggc ggcgattgcc gcgatggagg
     3721 ggcggattcc gctggtcgtc tccggcgacc tgcacgccat cgccgagggc cgcatgatgc
     3781 agtgcggggc gctcgatttc gcgaagaagc cggtcaatgt gatcttgtcg ggaccgctag
     3841 gcactggcga tcgcggctgg ccgtccgcct tccgtggcat cggcgcctcg ccgcccaatc
     3901 acttgaccat ggaggaggat ctgaagccaa tcgaggagaa tggattcatc cttgccgact
     3961 tctcgcccga aaacattacc gtgcgctact tccgtttcaa ctatcacaag cagccgggtg
     4021 aggtgatcga cacgctcgaa ccgttccgcg tgacagagct gaagcggtct tgacgctcac
     4081 gcttgatccg gcgacgggga ttgccgcgcg tcagtggccg cggccgtaga tttcataggc
     4141 cgtgaccacg atctcgaagg cgatcaggaa gatcacgacc aattccagcc gcagcgtgcg
     4201 cttcgcatcg atgatgtcgg caagcgtagt tgcggtgtcc gagatgaccg cgagtttccg
     4261 gttcaatgtt tccacacgct cgctgagctc atactcgtct tcaagtcgcg catagagtcg
     4321 ttccagatcg gggcggtccc acagcacgtc aggcttttcg ccgaccgcga cgcgacccga
     4381 cagccgatgt tggacgagca aggcgttgcc gagcagcttg agcaggtcgg tgcggtttct
     4441 cgacgttctg cccgcgcgcg caagttcgcg cgcaaacggc tccacggtgt cgacgacgtt
     4501 tgcaacctgt cgttcgtcgc gcgcgagcac gacgctcttg gcaagcgcgt cggcgacgac
     4561 cagaagccgt tccaacgaca tctctcgcac ggggatcggg ccgccgggcg ggatcggctc
     4621 atcggattct cttgccacct gaacctttgc aaattcctct tcgtacggag tgatcttgcc
     4681 gaaggtgcgc ggatgcaatc gctcgagaaa ctccgcttct tcgtcggcgg aaaggccgag
     4741 gaacacggca acgccgtagc gaaacaccac cgccaatccg tttctcccga tgcgcaccgc
     4801 aagcggcgtg ttcgacaaga cctgaccctc gaagccggcc gtgttgatgc ggtcgccgat
     4861 cagaagcgcg tgcgcggtga tttgctggga cgtcgggtcc gggcctgtca tcataggaag
     4921 gctagcagac tgtcggcgct ttttcgattg atgcggccgc gaaagcacgg ctccgcagcg
     4981 ctgcttttgc tttcgtcgca gagcttcggt acgctgccgt gaacccgatc accgcttcgg
     5041 ccgtatatct ctgcgacggc aaacctgagc gaaattcccc cgatgaccac gcgcggcggc
     5101 agcgacacgt tctatgccgg cataccggtg tttcgcggct tcaccaggtt gatggaaccg
     5161 tcgctctatt ccccgctgcc cggagactgg accgtcggcg tcgccgacat cgtggaatcg
     5221 acgaaggcga ttgcagccaa acgctacaag gcggtcaaca tggcgggcgc tgccgtgatt
     5281 gcggcggtga ccaatgcact cgaagggcgg gaatttccct tcgtgttcgg cggcgacggc
     5341 gcgagctttg cggttgctcc ggaggacctc gccgctgccc gcgaggcgct tgcggcgacg
     5401 gccgcctggg tcaaggcaga tctcgatctc gtcatgcggg tggcgctggt gccggtggcg
     5461 gcgatccgcg cgcagggcgc ggacgtgcgg gtggcgcgct tcgggccatc ggcgaatttg
     5521 tcctacgcga tgttttcggg cggaggtttg ggctgggcgg aggccgcgat gaagcgcggc
     5581 gagttcatgg tggccgaggc ggccgtgggc acgcagcccg atctgtcggg actgtcttgc
     5641 cgcttcgagg aaattcccgc aacacgcggt ctcattctct cggtgctggt gttgccgacg
     5701 caaggcgccg atctgcccgc cttccgcaag gtgatcgagg acatcgtcgg tctcgtcgag
     5761 aagagcccgg attccgggcg gccggtgcca ccgggcgggc cgccgctgcg atggccgccg
     5821 cagggcgtcg agtacgaggc gcgcgcggcg cgcggcggct cgcttgcgaa gcgtcgcgtc
     5881 gtcgtgctcg ccgtcacgtt gtgggcctat gtggtgatgc ggttcggcat caaggtcggc
     5941 aaattcgtgc cgaagaccta cttgcagcag gtggtcgaaa attccgactt ccgcaaatac
     6001 gacgatggcc tgcgcatgat cctcgactgc aatgaggaac tcgcacatgc gctgaccgag
     6061 cgcatcgccg cggcggccgc gagcggcacg gttcgctacg ggctgcatcg ccaggacgcg
     6121 gcgatgatga cctgcttcac gccctcggtc atgcgcgcgg accacgtgca tttcatcgac
     6181 ggcgcccgcg gcggctacgc ttccgctgca atcgcgctga aggccatgac ggcatgaagc
     6241 tcgagggaca tggtcagcgt ccggcgccca gacctggatc gcccgagacg cgccatttcg
     6301 ccggtccgcg ggtcggcgtc ggcgaaaatg cggtgtctaa gaccttcgga accttctata
     6361 agggggtccg ttaagttcac gtctcccaca gttcttggac aacaccccat gacctttacg
     6421 cttccccccc tgccttacgc ttatgacgcc ctcgggcctt atatgtcgaa ggaaacgctg
     6481 gaattccacc acgacaagca ccatcaagcc tatgtcacca acggcaacaa cgcgatcaag
     6541 gggaccgaat tcgaaggcaa gtccctggag gagatcgtca agggctcgtt cggcaagaat
     6601 gctgcggtct tcaacaatgc cggtcagcat tacaaccaca tccacttctg gggctggatg
     6661 aagcccaagg gcggcggcag caagttgccc ggcagcctcg agaagaagat caacgaggac
     6721 ctcggcgggt tcgagaagtt caagaccgat ttcatcgccg ccggcgtcgg tcagttcggc
     6781 tccggctggt gctggctgca ggtgaagaac ggcaagctcg aaatcgccaa gaccgccaac
     6841 ggcgagaatc cgctggtcca tggcgcgacc ccgatcctcg gctgcgacgt ctgggagcac
     6901 tcctattaca tcgactatcg caaccgtcga ccggactatc tgaaggcgtt cgtcgaaaat
     6961 ctcgtcaact gggattacgt cgagcagttg ttcgccaaga caagctgatc gcgctcacgg
     7021 ccgtcattgc gaggagcgca agcgacgaag caattcagta tcttcattgg cgctggattg
     7081 ccgagcttgt catcgggccg cgcttcgcgc gaacccgttt gctcggatga aggtcctcaa
     7141 agccaaggcg gtggcgcaag ctgccgcctt ttccttgtgc ccctggcgac ccaatcgcgc
     7201 atgggcccct caggcgagcg catgggtctt gccgcacttc cgtttgcatc gcctcccggc
     7261 cgcaattaat caggttgggc tgttcatgtc gcgccagacc aagttgttgt cagaccttcc
     7321 cttgaaagac ccggcccaag cctagtggtc cgattcgaac gtcaaatccg ccccacgaac
     7381 gacgccgaag gcgaaccgcg tccggcacgc gcgcgaaaac ctgtcggctg gtcgatgatc
     7441 acgatcgccg tgctggttgc ggtcagcgcc gcgctggtgt ggcggcgcga cggtcttccc
     7501 ggcgtgctcg atatcctgac ccatgatgtt tcgctgttcg gcggcatcct gccgcgcgtc
     7561 cttgccggtt gcctgctcgg cgccttcatc gccgaaatcc tgccgcacga caaagtctcc
     7621 cgctcgctcg ggccgaaatc gggcgtcaag ggccttctga tcggcaccgc gttcggcgcg
     7681 atcctgcccg gcgggccgtt cacggcctat ccggtggcaa gcgcgctgct tgcggtcggc
     7741 gccgatttcg gggccaccat tgcgatggtc gtgagctgga cgctgatcgg ctacggccgt
     7801 gcggttgcct gggaattgcc gatcatgggc accgatttca cgctgtggcg catcgtgatc
     7861 tcgttgccct tgccggtgct cgccggcgcg ctcgggcgct ttgtctatgc gcggatgtat
     7921 cctgccaaag gcacggacga cagcgaatga gcgccgcgct tctcatcgac atcatgctgt
     7981 ggggttcggt cgcggcggtc ggcttgatcg cctatcggcg cggcccgacc gtgctgaacg
     8041 cgtcgctgcg cgaaggcgcc atggatttta tcaatatcat accgcgcatc gcgctgggcg
     8101 tgatcggctc tggctacatc gccgccgtca ttcccccgga ggtcattaca acctggctgg
     8161 gaccgaatag cggatggttc ggggtggccg cggccgttct cgccggcgcc gcgacgccgg
     8221 gcggaccggt ggtgggtttt tcgatcgggg cggtggccct gaagtccggc gggggcgcgc
     8281 cccaggtgct ggcttacgtc attgcctggg ccctgttcgc ctttcagcgc ctcctgttat
     8341 gggaaatccc gttcatgccg gcccgtttcg tctggtttcg ggctgccgtc tccgttcctt
     8401 tcccgttcct ggcggccgcc atcgccatgg cgatcgggaa gccctgatgc cccctacgcg
     8461 gcattgcctg ggggctgtaa cgttataata taacaatgca ccatggctca cccgcattcc
     8521 catagccacg agcacggggc ggatcacgcc catgcgcacg gacatgtgct tgcccatagg
     8581 cacggtcctg cctcgccgca tcccgcgcag gccgcgacat ggtcgatcct gcgcatgccc
     8641 gttgcctcgc gcctagcggc ggcgcttctc gtctgcgccg cgctctgggc tgtcgttctg
     8701 ctcgcgatga gatgagcatg gcgccaaaac tcatcttcca gaacgtcacg ctcggctatg
     8761 accgccaccc ggctgtgcat catttgtccg gcgaggtcgt ctccggcgcg ctgcttgcgg
     8821 tgatgggacc gaacggcgcc ggcaaatcga cgctgctgcg tggcatcgcc ggcatcctca
     8881 aaccgctgtc aggttctatc tcgctcggca gcatcgacac tttggacatc gcctatttgc
     8941 cgcagaccgc cgagatcgat cgcagttttc cggtctcggt attcgatttc gtcggcaccg
     9001 ggctgtggcg cagggtcggt ttttttggcg gcatcggcaa ggagcagcgc aaccagatcg
     9061 aaagcgcgct cggtgcagtc ggcctcaccg gcttcgagaa tcgtccgatc ggcacgctgt
     9121 ccggggggca gatgcagcgc ctgttgttcg cgcgcgtgct gttgcaggat gcgcgcctga
     9181 tcgtgctgga tgagccgttc aatgccatcg atgccaagac ctgtgccgac ctgcttgcgc
     9241 tggtgaaaca ctggcacggc gaagggcgca ccgtgctggc ggcgctgcac gatctggaaa
     9301 tggtgcgcgc ccattttccc gacacgctgc tgctggctcg cgggccggtg gcgtggggcc
     9361 cgaccgcgca cgtgctgacg gcggacaatc tcctcgtcgc gatgcggatg tgcgaagcct
     9421 tcgacgacgc cgctgccgcc tgcgcggtgg acacgccgtc gcgcgcggcc tgaccgtcat
     9481 gcttgccgac gcgctgatcg cgccattcac cgaattcgaa ttcatgcgcc gcgcgctggc
     9541 tgcgatcgtc gcgctgtcgc tcggcggtgc gccgatcggc gtgttcctgc tgctccggcg
     9601 gatgagcctg gtcggcgatg ccatggcgca tgcgatcctg ccgggagcgg cgatcggctt
     9661 tctgctgtcc ggcctcaatc tcttcgcaat gacgacgggc gggctgatcg cgggttttgc
     9721 cgtcgcaatt ctcgccggtg tcgtagcccg ctcgaccgga ttgaaggagg atgcctcgct
     9781 ggcgaccttc tatctcgcct cgctcgcgct cggggtgacc atcgtctcga tcaagggcac
     9841 caatatcgat cttctgcatg tcctgttcgg tgacatcctg gccatggacg accagaccct
     9901 gctgctggtc gcgatcaacg ccaccatcac gttgctcgtt ctggcggtga tctaccggcc
     9961 gctcgtgatc gagtgcgtcg atccgctttt cctgcgcacg gtgagccggg ccggcgcacc
    10021 cgcgcatctc gcgttcctgg cgcttgtcgt gatcaacctc gtcagcggct tccaggcgct
    10081 cggcacgctg cttgcggtgg gcctgatgat tctgcccgcc ggcatcgcgc ggttctggtc
    10141 gcgcgacgtg acgccgatga tttgcattgc cgtgttgagc gcgatcgtgt cgggctatgc
    10201 gggcctcgtg ctgtcgttcc agacccgcat accctcgggt cccgcgatca tcctggtggc
    10261 ggctgcactt tacatcgcct ccatcctgtt cggcagcgtc ggcgggctgg tgcggcaact
    10321 cttccctcgc cgacacctgg aggcctaggg ccatgcgacg gctcatggtg tggtcgatct
    10381 gtctggccgc gatcgtctgt ccggcgcgcg cgcaggaccg cctcaacgtg gtcgcgagct
    10441 tttcgatcct cgccgatttc gtcaaaaatg tcggcggcga caatgtcgac gtgacgacgc
    10501 tggttggccc caatggcgac gtgcatgtct acgcgcccgc gccaagtgac gcgaagaaga
    10561 tcgcggacgc gaaactgctc gtcatcaacg gcctcggcct tgaaggctgg ctgccgcggc
    10621 tgttgcagtc ctccgggagc aaggcgccga tcgtcgttgc gacccggggc attacgcctc
    10681 tgaagctcgg ctccgatgcc gatccgcatg cc
//
LOCUS       DATLJT010001295         1526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3092177,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001295 DATLJT010000000
VERSION     DATLJT010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3092177"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VM910_29765"
     CDS             <1..904
                     /locus_tag="VM910_29765"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI66730.1"
                     /translation="DGSYATRGYGEMDRLMWDLDGRLENLRRRGIDLQLISPSPRTIS
                     DHEHVIGVEIARLMNRETANLVKRGGGRLGGMGVVPLGEPEKAADEIRRAVGEYGFQG
                     ACIPTSAAGRPLDLPVFGNVWDTLEELGLLVFMHATTAITREPLGQYTLNTVIAWPTE
                     VTIAAMRLIFSGVIERHPGLRLVLSHGGGTLPYLAGRLDLAYGAPKYEANPACRANIT
                     KPPSHYLTQFYYDTVVASPASLRFLIDLVGADRVMFGTDFPYEIGDADGAIALPVLEQ
                     LPSAQREKILGQNAREVLRRVRSG"
     gene            1119..>1526
                     /locus_tag="VM910_29770"
     CDS             1119..>1526
                     /locus_tag="VM910_29770"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66731.1"
                     /translation="MSHLHNVHRKVCVIAALHLIAGAVISGSAATSSAQETARPSILG
                     SAASHASSSPIMSNDGPVPLRLAQAQVGRPMQKITFLLDFTPYGKHAPFFAALDKGFW
                     KDAGFDVTIVKGEGSATTISSYAAGAVDFAFSDT"
BASE COUNT          274 a          475 c          476 g          301 t
ORIGIN      
        1 cgacggcagc tacgcaaccc gcggctacgg cgagatggat cggctgatgt gggacctcga
       61 tgggcggctc gaaaatctgc gccgtcgcgg catcgacttg cagctcattt caccgtcgcc
      121 gcggaccatc tccgatcatg agcatgtcat cggcgtcgag attgcacgac tcatgaatcg
      181 ggaaacggcg aatctggtca agcgcggggg cggccggctt ggcggcatgg gcgtcgtccc
      241 attgggcgag ccggagaaag ccgccgacga gatccgacgg gcggtcgggg agtatggctt
      301 tcagggagcc tgcataccca cgtcggcggc gggcaggccg ctcgacctgc cagtgttcgg
      361 gaacgtatgg gacacgctgg aggagttggg tctgctcgtc ttcatgcatg cgacaacagc
      421 gatcacacgc gagccgctcg gccagtacac cctcaacacg gtgatcgcat ggccgaccga
      481 ggtgacgatc gcagcaatgc ggctgatctt ttccggcgtg atcgagcgcc atcccgggct
      541 ccgattggta ttgtcccacg gaggcggaac gcttccctat ctcgcgggcc gcctcgacct
      601 tgcctatggc gcacccaagt atgaggcgaa ccccgcttgt cgggccaaca tcacgaagcc
      661 gccgagtcac tacctcaccc agttctacta cgacacggtc gtagcaagcc ctgcttcgct
      721 gcggtttttg atcgaccttg tcggggccga tcgcgtcatg ttcggcaccg actttcccta
      781 cgagatcggc gatgcggacg gcgccatcgc gctgccggtg cttgagcaat tgccgtcggc
      841 ccaacgtgag aaaattcttg gacaaaatgc gcgcgaggtt ctgcgacgcg tacggtccgg
      901 ttgaccgatg atcgcatgcg agcggtggcc gcgctccgct gcgacaccgc ggcgatgcaa
      961 tcggctacat cagcattgac accggctgac ggggctgttt cgcttggtcg ccgttatgaa
     1021 acgcgtgtcc ggccggggtt gccagcgata cgaggatgtc gtcgcgaagg ctcaacgcgg
     1081 tccgtgatga ccccgtatct caatcgtgga gattcctgat gagccatctg cacaacgtgc
     1141 atcgaaaggt ctgcgtcatt gccgcccttc atctcatcgc tggcgctgtc ataagcggct
     1201 ctgccgccac cagttctgcg caagagacgg caagaccctc aatcttaggt agcgctgcga
     1261 gccatgcatc gtcttcgccg atcatgtcga atgatggccc ggtgccgctg cgtctcgcgc
     1321 aggcgcaggt cggccgcccg atgcagaaaa tcacttttct gttggatttc acgccctacg
     1381 gcaagcatgc gccgttcttc gctgctctcg acaagggctt ctggaaggac gccggtttcg
     1441 acgtcacgat tgtcaagggc gaagggtccg ccacgaccat ttcctcatat gcggccggcg
     1501 cggtcgactt cgcgttctca gatacg
//
LOCUS       DATLJT010001296         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1714142,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001296 DATLJT010000000
VERSION     DATLJT010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714142"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VM910_29775"
     CDS             complement(<1..686)
                     /locus_tag="VM910_29775"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVI66732.1"
                     /translation="MQIDPLVKPRSIAIVGATDRVGPARSVIESLGAIGFAGPIYPVN
                     PKYQTVRNLVCYPSLTDLPEAPDVVVFSIRKPLLPEQVRLAVKRGARAAVIYDAGFAE
                     LGEEGARQQAEIAGLCRDAGMPVCGPNCMGILNPTARSTTYKQTVMDPSGLAGNVGIV
                     SQSGSVCIALLSDLRRYGVSLSVSAGNEAVTRTVDYLEYLIDDPATKVIATFTETVRE
                     PERYVAALDRA"
     gene            complement(692..1468)
                     /locus_tag="VM910_29780"
     CDS             complement(692..1468)
                     /locus_tag="VM910_29780"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66733.1"
                     /translation="MARHVPLLLASLAAIFLVAPMTIIVPMSFSKAISFEFPPPGYWT
                     GYYAQYFSSHSWLEATANSFIIAFGSMALTLIAALPAAFGFVRYRFRGKSMLNLIMML
                     PIIVPAVVSALGYYSFLSPLRLVGTHAGMIIAHSVLSIPVAFLVIAAALKGFDRNLER
                     AAMSAGAGPLRTFLWVTLPVLRPGILVGALFAFLHSFNEAVVAIFIAGRDASTLPKKM
                     FESIRLESDPVIAVVSTLLTGAVLLGVLISLFFRQRPAQG"
     gene            complement(1474..>2167)
                     /locus_tag="VM910_29785"
     CDS             complement(1474..>2167)
                     /locus_tag="VM910_29785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66734.1"
                     /translation="YRRFFTVPLYANVMVNTFRTSLIVTLACLALGYPLAYVMARRND
                     AVAVVFLVAVGLSFWTGFVVRTYAWLIILGNKGPLAATYALAGWGKPPQLLFTSFSST
                     LGMTHVLLPYMVLALYGVMRKIDPSYMRAAEGLGARPFGAFRLVFLPLSLPGVVNGCV
                     LVFTMCLGFYITPILLGTPKDMMISQLISQQIEDLLAWGFASAIAVVLLASTLVLLGV
                     YNRFAGLDRLWG"
BASE COUNT          455 a          638 c          762 g          312 t
ORIGIN      
        1 gcgcggtcga gcgcggcgac gtagcgctcg ggctcgcgca cggtctcggt gaacgtcgcg
       61 atcaccttgg tggcgggatc gtcgatgaga tattcgagat agtcgaccgt tcgggtgacg
      121 gcctcgttgc cggccgagac cgagaggctc acgccgtaac ggcgcagatc ggacagcagc
      181 gcgatgcaga ccgagccgct ctgggacacg atcccgacat tgccggcaag gccgctcgga
      241 tccatcacgg tctgcttgta ggtggtcgag cgcgccgtcg gattgagaat gcccatgcag
      301 ttcggcccgc acacgggcat gcctgcgtcg cggcacaggc ccgcgatttc ggcttgctga
      361 cgcgcccctt cctcgccgag ctcggcgaaa ccggcgtcgt agatgacggc ggcgcgcgcg
      421 ccgcgtttca ccgccagccg cacctgctcg ggcagaagcg gcttgcggat gctgaaaaca
      481 acgacgtcgg gcgcctccgg cagatcggtc aggctcgggt agcagacgag attgcggacg
      541 gtttgatact tggggttgac tggatagatc ggtccggcaa agccgatcgc gccgagcgac
      601 tcgatgacgg agcgcgcggg cccgacgcga tccgtggctc ccacaattgc gatcgaacga
      661 ggtttgacca gcggatcaat ctgcatatcg ctcacccttg ggcgggcctt tgccgaaaga
      721 acaaggagat caggacgccg agcagcaccg cgcccgtgag cagcgtcgat accacggcga
      781 tgacgggatc ggattccagc cggatcgatt cgaacatctt cttcggcagg gtcgatgcgt
      841 cgcggccggc gatgaagatc gcgaccaccg cctcgttgaa ggagtgcagg aacgcgaaca
      901 gcgccccgac caggattccg ggccgcagca caggcagcgt cacccagagg aaggtgcgca
      961 gcggtccggc tccggcactc atcgcggcgc gttcgaggtt gcggtcgaag cccttgagcg
     1021 cagcggcgat gactaggaag gcgacgggga tggataacac gctgtgcgcg atgatcatgc
     1081 cagcatgggt ccccacgaga cgcaaggggc tcaagaagct gtaatagccg agcgcgctca
     1141 cgaccgcggg cacgatgatc ggcaacatca tgatgaggtt gagcatgctc ttgccgcgga
     1201 agcggtagcg cacgaagccg aaggcggcgg gcagcgctgc aatcagcgtg agcgccatcg
     1261 atccgaacgc gatgatgaag gagttggcgg tggcctcgag ccaggaatgg ctcgagaagt
     1321 attgcgcgta atagccggtc caataacccg gcggcggaaa ctcgaacgag atcgccttcg
     1381 agaacgacat cggcacgatg atcgtcatgg gcgcgaccag gaagatcgcg gcgagcgacg
     1441 ccaacaggag tggaacgtga cgcgccatcg cccctatccc cacagccgat ccaggccggc
     1501 aaagcgattg tagacgccga gcagcacaag cgtggacgcc agcaggacga ccgcgatcgc
     1561 cgaggcgaag ccccaggcca gcagatcttc gatctgctgg ctgatgagct gggagatcat
     1621 catgtctttg ggcgtgccga gcaggatcgg cgtgatgtag aagccgaggc acatggtgaa
     1681 cacgagaacg cagccgttga cgaccccggg caggctgagc ggaaggaaga cgaggcggaa
     1741 tgcgccgaag ggacgcgcgc cgaggccctc ggcggcccgc atataggaag gatcgatctt
     1801 gcgcatgacg ccatagaggg cgagcaccat gtaggggagc aggacgtggg tcatcccgag
     1861 cgtggagctg aacgaggtga agaggagttg cggcggcttg ccccatccgg ccaaggcgta
     1921 ggttgcggcg agcggtccct tgttgccgag aatgatcagc caggcatagg tgcggaccac
     1981 gaagccggtc cagaagctca ggcccaccgc gacgagaaag acgacggcca cggcgtcgtt
     2041 gcgtcgcgcc atcacgtagg ccagcgggta accgagcgcg aggcatgcca gcgtcacgat
     2101 caacgacgtc cggaacgtgt tgaccatcac gttggcgtag agcggaacgg tgaagaagcg
     2161 gcggtaa
//
LOCUS       DATLJT010001297         9180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_565668,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001297 DATLJT010000000
VERSION     DATLJT010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565668"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VM910_29790"
     CDS             <1..737
                     /locus_tag="VM910_29790"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVI66735.1"
                     /translation="GAGLSSRDLNRVVACVALAGASEPSQLEIARGHEHPYCMAVFVT
                     DARAACVGAHGGRDGGIIVIGTGSIGWAELNGQQYQVGGWGWPISDEGSGAWLGCEAL
                     RRTLWAHDGRMPWSALLRSLFARFQSDPHTIVHWMTGASPKDFAAFAPEVVEHALAND
                     PVAVELLRLAGGHVDALAQRLIDFDADRLSLVGGLAASIEPQLADATRHHLVAPRGDA
                     VAGALQLARNAAESLAPGLTALGRSR"
     gene            734..1765
                     /locus_tag="VM910_29795"
     CDS             734..1765
                     /locus_tag="VM910_29795"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVI66736.1"
                     /translation="MSDNRVNMEADWQKSPDAVRRQPKALEAPLAELLRCLKREPPSF
                     VLTSARGSSAHAATFGKHLIERHLGIPVGAFAPNIATIYKQRLKLDGQLFLTVSQSGR
                     SDDLIESVEMANAAGALTATIVNDTDSPLAKSSAIVLPMAAGPELSVAATKTFITSLS
                     ALLHVVAGWAGLDELRAAIERLPDRLTAAMRLDWSPALNALCEATSLVSLGRGPTLAI
                     AREAALKLKETSNLHAEAFSGAEFLHGPIALVESSYPALLFLPTDHAAVGLRALAADL
                     RRKNASVFMTDHGEQQAGTLPALAPDHPDTDAVCLIQSFYALAIRVAQCRGINVDLPR
                     HLQKVTRTR"
     gene            1762..2943
                     /gene="nagA"
                     /locus_tag="VM910_29800"
     CDS             1762..2943
                     /gene="nagA"
                     /locus_tag="VM910_29800"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HVI66737.1"
                     /translation="MNSLRPHAIAADHVFDGTVVREHTAVVVDGARIVDLVATSELPR
                     TMLLRALPDDAWLAPGFIDLQVNGGGDVLFNDQPTVEGIRSIAAAHRKFGTTGLLPTL
                     ISDSAEKMRLALDAASAAASEEGGVLGVHLEGPFLSSEKPGVHDARQIRSPSAEELTM
                     LTAPRNGVLLVTLAPEVVPPGVIAQLVAAGIRVSLGHSMASYRQTRAAMAEGLTGFTH
                     LFNAMLPLSSREGGPIAQALESPDAWYGLIVDGVHVDPAMLRLALRGLGHPMLVSDAM
                     PPVGGSSANFSLHGKNITARDGYCVTDDGTLAGTVLDMAAAVRNCIRLLGVALPDALR
                     FASAHPATFLGLEHMLGKLAPGYRADLVAFDPNDITVLATWVAGRDDHSFRPRGPLGR
                     S"
     gene            3180..4277
                     /locus_tag="VM910_29805"
     CDS             3180..4277
                     /locus_tag="VM910_29805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66738.1"
                     /translation="MRQFLAGISNCPTALSVALLAVAVALLAVAMAPSSPDVALSDAS
                     GSGALTSAAPPTPACGSNELELHEPSPDSEPGCATAQRTPAEVAEARAYLIETASPGY
                     TMTLQGAELAIGRLHPEFAVRLENAIREARSAGLPSAGVFSAYRPPAFGVGGFSDKFN
                     SLHTYGLAVDMRGIGNPGSPEAQLWHQIAAKNGVVCPYGPRDHAEWNHCQPTSVKIIS
                     AQNPLRETVSSAGPFDLESMFEAGNPIIEDMASAADSLSRAAPTPVRALEANADGLKP
                     IRHLAASRNTKRNSPAATRRVAAKSVAHAKPNSPAARQRVAAKSEPHAKPGRRVGGAP
                     TVAVEEARRKSKSGAGVALNRRAHRAAARET"
     gene            4719..4949
                     /locus_tag="VM910_29810"
     CDS             4719..4949
                     /locus_tag="VM910_29810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66739.1"
                     /translation="MSNIPPTRSSSPAAERMRLYRKRRRNGMQYVRIPLHVTDIDDLI
                     RTGRLKEEQRHDAGALQTAVLDLVYRAMEHRT"
     gene            5546..5650
                     /locus_tag="VM910_29815"
                     /pseudo
     CDS             5546..5650
                     /locus_tag="VM910_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(5686..7155)
                     /locus_tag="VM910_29820"
     CDS             complement(5686..7155)
                     /locus_tag="VM910_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVI66740.1"
                     /translation="METARGFVTAMALTEADRNRQLACPSIPSGTMAAAVTVAFATWS
                     LFFAPAVLAQPTPDSLGEPLVDKAAFGDWRTDAPLVRRKITALPPPYATRSASNFPRV
                     IGKPASAAPKVPPGFQVELFASNLNDPRVVRIAPNGDIFIAESEPGRIRVLRAADGAD
                     KPSNNDVFVSGLDQPFGIAFYPPGSDPHWIYVANTGSVVRYPYRSGDLKARGKAEVIV
                     RELPHAGGRSVQRGHITRDIVFSKDGRQMFVSVGSASNDGEGMGKRNAAAIARWEAEH
                     GLGSAWGNETDRAAVLVFDPEGGHRRVFASGLRNCVGLAVHPMTGDVWCSTNERDDLG
                     DDLVPDFVTRVRGGGFYGWPWYYLGANEDPRHRGERPDLKDKITVPDVLIQAHSASMG
                     MIIYDGDQFPSDYSGDVFAAEHGSWNRAKRTGYKVIRVRLNDGVPTGEYEDFMTGFVV
                     NDSSVWARPVGVAAAHDGALLVSEDGNGTMWRVSYRGGP"
     gene            7785..8609
                     /locus_tag="VM910_29825"
     CDS             7785..8609
                     /locus_tag="VM910_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI66741.1"
                     /translation="MEAFICTACGTQYTPSSVAPRQCVICEEERQYVPPRGQTWTTLA
                     ALTAGHFNSYRQYEPGVIGIGTQPQFAIGQRALVVCTPNGNVLWDCIAMLDPATIALI
                     NGLGSLKAIAISHPHFYTTMVEWSRAFGEIPIHLHADDHRWIMRPDPAIQLWNGETLK
                     LMPDLTLIRCGGHFRGGTVLHWAKGAGGRGVLCSSDIATVTTDRKFLSFMRSYPNLIP
                     LSAKQVTGIAVALEPFQFDTIYGHYFDRVITSGGKRTLEISVKRYVDAISGVYEHE"
     gene            8911..9057
                     /locus_tag="VM910_29830"
     CDS             8911..9057
                     /locus_tag="VM910_29830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66742.1"
                     /translation="MNVADARARMEQQGAVPLTATPEQFDVIIRNDAERNVRVLWEAG
                     VAAN"
BASE COUNT         1716 a         2913 c         2855 g         1696 t
ORIGIN      
        1 gtggggccgg attgtcgtcg cgcgatctca acagggttgt ggcctgcgtg gcactggcgg
       61 gcgccagcga acccagccag ctcgagattg cgcgaggcca cgaacatccc tattgcatgg
      121 cggttttcgt caccgatgcc cgggccgcat gcgtcggcgc acatggcggg cgtgacggcg
      181 gcatcatcgt gatcggcacc ggcagcattg gctgggcgga gctcaatggg caacagtatc
      241 aagtcggcgg ctggggttgg ccgatttcgg atgaaggaag cggcgcttgg cttggctgcg
      301 aggcgttgcg ccgcacgctg tgggcccatg atgggcgcat gccgtggtcg gcgctgctgc
      361 gttcgctctt tgcaagattt cagtcggacc cgcacacgat cgtgcattgg atgacggggg
      421 catcgccaaa ggatttcgcc gccttcgcgc cagaggtcgt cgagcatgct ttggccaacg
      481 accctgtcgc ggttgagctc ctgcggctcg ccggcgggca tgtcgatgcg ctggcccaac
      541 ggctcatcga cttcgacgcg gatcgcctgt ctctggtcgg cggtcttgcg gcgtcgatcg
      601 agccacagct cgccgacgcc acacggcatc atcttgttgc gcctcgcggt gacgcggtcg
      661 ccggcgctct gcaactggcc cgcaatgcgg cagaatcctt ggctcccggc ctcactgccc
      721 tggggcgatc ccggtgagcg ataaccgcgt caacatggaa gcggattggc aaaaaagccc
      781 cgacgccgtg cggcggcagc cgaaggcgct cgaagccccg ctggctgagc ttctgcgttg
      841 cctcaagcgc gagccgccga gctttgtcct taccagcgcg cgcgggagct cggcccatgc
      901 ggcgacgttc ggaaagcatc tgatcgagcg ccatctcggc atcccggtcg gcgcttttgc
      961 gccgaatatc gctaccatct acaagcagcg cttaaagctc gacggtcagc tcttcctcac
     1021 ggtctcacaa tccggaagga gcgacgacct catcgagtcg gtcgagatgg ccaatgccgc
     1081 aggcgcgctg accgcaacca tcgtcaacga taccgacagc ccattggcga aatcctccgc
     1141 cattgtgctc ccgatggccg cggggcccga gctcagcgtg gccgcaacga agaccttcat
     1201 caccagcctt tccgccttgc ttcacgttgt cgcgggctgg gccggcttgg atgaattgcg
     1261 ggcggcgatc gaacgcctgc cggatcgtct cacggctgcc atgcggctcg attggagccc
     1321 agccttgaac gcgctgtgcg aggcgacgag cctcgtgagt ctgggacgcg gcccgacgct
     1381 cgcgatcgcg cgtgaggctg ccttgaagtt gaaggagact tcaaatcttc atgccgaagc
     1441 gttcagcggc gctgaattcc tccatggtcc gattgccctc gtggagtcgt cataccctgc
     1501 cctcctgttt ctacccactg accacgccgc agtggggctg cgcgcgctcg ctgcggatct
     1561 ccgccgcaag aatgccagcg tattcatgac ggaccacggg gagcaacagg cgggtacttt
     1621 gcccgccctc gcgccggatc accccgatac cgatgccgta tgcctgatcc agagcttcta
     1681 cgcgctggcc attcgcgtcg cccaatgccg cggcatcaac gtcgatcttc cgcgccattt
     1741 gcaaaaggta acgcggacca gatgaatagc ttgaggccgc acgccatcgc cgcggaccac
     1801 gttttcgacg gcaccgttgt gcgcgagcat accgccgtgg tcgtggacgg tgctcgcatc
     1861 gtggatctcg tggcgacgag cgagttgccg cggaccatgc tccttcgcgc gctgccggac
     1921 gatgcgtggc tggcgccggg attcattgac ctgcaggtga acggcggcgg agacgtgctg
     1981 ttcaacgatc agccgaccgt ggaaggcatt cgcagcatcg cagcggcgca cagaaaattt
     2041 ggaaccaccg gtttgctgcc gacgctcata agcgacagcg cggagaagat gcggctggct
     2101 ttggacgcag ccagcgccgc ggccagcgaa gaaggaggcg ttctcggcgt tcacctcgag
     2161 ggtccctttt tatcttccga aaaacccggc gtgcacgacg cgcgtcagat cagatcgccg
     2221 tccgcggagg aactcacgat gctgacggcg ccgcgcaacg gcgtgctgct cgtgacccta
     2281 gcgcccgaag tcgtgccgcc gggagtcatc gcccagctcg tcgcggccgg cattcgcgta
     2341 tcgctcggtc attccatggc ctcttaccgg cagacccgcg ctgccatggc ggaggggctc
     2401 acgggcttca cgcacctctt caatgccatg ctacccttgt ccagccggga aggaggacca
     2461 atcgcgcagg cactcgaatc gcctgacgcc tggtatgggc ttatcgtcga cggcgtgcat
     2521 gtcgatcccg ccatgcttcg cttggcgctg cgaggcctcg gccatccgat gctcgtcagc
     2581 gatgcgatgc cccctgtcgg cggaagctcc gccaatttca gcctgcatgg gaaaaacatc
     2641 actgcgcggg acggctactg cgtcacggac gatggaacgc tcgccgggac ggttctagac
     2701 atggcggctg ccgtcaggaa ttgcattcgc ctgctcggag tcgcattacc ggacgcgcta
     2761 cggtttgcat cggcgcatcc ggccaccttc cttggtctcg aacatatgct cggcaagctt
     2821 gcccccggat atcgcgccga cctcgtggcc ttcgacccga acgacatcac ggttcttgcg
     2881 acgtgggttg ccggacgtga cgatcattca ttccggccgc gtgggccgct gggccgttcc
     2941 tgataacctg gcgcctcttt gttcgatgta gtgtcgctgt gtcaggcggg ccgggagttc
     3001 cagctggaac ttcgtcgctc cgttttgccg ttcgatcacg ttttgaatcg aaagaaacat
     3061 tcattcaata ggctgagcgt cagctcgcgc aactaatcac attgtcgacg caatcgccgt
     3121 caatgtgcgg ccggtccgcg agcgcggaca ctttcctgac aatttggcac ggggatctca
     3181 tgcgacaatt tctcgccgga atttcgaatt gcccgacggc actttctgtg gcgctgttgg
     3241 cggttgctgt ggcgctgttg gcggttgcta tggcgccttc ctcgccggac gtggccttgt
     3301 cggatgcgtc gggatcgggc gctttgacct ccgcagcgcc gccgacgccc gcctgcggtt
     3361 ccaatgaact cgagctgcac gagccctcgc ccgacagcga gcccggctgc gcaaccgctc
     3421 aaagaacacc ggcagaggtt gcggaagctc gcgcttatct gatcgagacc gcgagccccg
     3481 gttacacaat gacgttgcaa ggcgccgagc tcgcgatcgg acgtctgcat cccgaattcg
     3541 ccgtccgcct ggagaatgcg attcgggaag cgcggagcgc cgggctgccg tccgcaggag
     3601 ttttctcagc ttatcgaccg cccgcattcg gagttggcgg tttctccgac aagttcaact
     3661 ccctgcacac ctacggactc gcggtcgaca tgcgcggcat cgggaatcct ggctcccccg
     3721 aagcgcagtt gtggcaccag atcgctgcca agaacggcgt cgtttgtccc tatggtcctc
     3781 gcgaccacgc cgaatggaat cattgccagc cgaccagcgt caagatcatc tcggcccaga
     3841 accctttacg cgagacggtg agctccgcgg gcccatttga ccttgagagc atgttcgagg
     3901 ccggcaatcc cattatcgag gatatggcga gcgcggccga ttcgctgtcc cgggccgcgc
     3961 ccacgccagt gcgcgcattg gaagcaaatg ccgacggtct taagccaatc cggcatcttg
     4021 cggccagccg caacacaaag cgaaattcgc ccgccgccac gcggcgtgtc gccgctaaat
     4081 cggtggcgca tgcgaagcca aattcgcccg ccgctaggca acgcgtcgcc gctaaatcgg
     4141 agccgcatgc gaagcccggc cggcgcgtag gcggagcgcc gactgtcgcg gtcgaggagg
     4201 cccgccggaa gtcaaaatcc ggagcgggcg tagccttaaa tcgacgtgcg caccgagccg
     4261 cggcgcgcga gacgtagcac agcaatgaaa gatcgcagtt ctcggaccat aacccaggtg
     4321 aatcaattca ggcaaaaatg cccgctttaa aattcggcat cgttgccggc gcctttctga
     4381 aaaaaattgg tgcccagggg cggcgtgcca tgggcaagca atatcagcgc gttgcattgg
     4441 caaaccccca cccgcggcag ctttgacccc caaaggcttt ccggcgcgct ggcaaaccca
     4501 actcgagctg ccgagttttg tcgccgctgt ttaaccgggt cgacagcgtg acgggcgtcg
     4561 ccgtgctttg agactcggct ggccgatgac agcgcggcac ggcccgccca tgtggggacc
     4621 atgatctgga cgagccgagc cgcgctaggc gacggtgggc gtaatggcac cgatagggta
     4681 attcataaac cgtatgaaga gccgtatcga gcaggaccat gagcaatatt ccccctacac
     4741 gctcatcctc cccagccgcc gagcgcatgc gcctctaccg caagcgacgc cgaaacggga
     4801 tgcagtacgt tcgcatcccg ttacacgtaa cggacatcga cgacctcatt cgcacggggc
     4861 gtttgaagga ggagcagcgt cacgatgccg gggcgcttca aactgcagtt ttagatcttg
     4921 tctaccgggc gatggagcac cggacgtgac gtgtaacgac ggcggccgga cggtggcttg
     4981 aagatgttag gggaccccgt tgacgggacg ttacacgtaa cggtggggcg gtccgcctac
     5041 ctgcgggcgt cgaccgccaa agggcctcct aggcaggatg aatctttatc cacttctgac
     5101 agacggccgc atctgacgcc attacgggcg gaaaacgtcg gcaccggctg caatccgttt
     5161 tgaaaacagg aaatttgccg tcgattttgg gggcaggtct ggtctcataa gtccccgcca
     5221 gcgatttgag cactgccggc agagcggccg ttacctgtaa cggcggggcc tcgcgcatcg
     5281 cgttacacgt aacggggacc taccggaccg actggagccc gcgatgaggc ccgccttgca
     5341 atgcggcacg cctctgcagc gggccccgtc ggaccttaat agcaggacct cgggatcggg
     5401 ccacacccag gctgtcaacg ggcgcgatgc tggggcgagc ttgatgaagc cgcgccgggc
     5461 ggcctccagc gcgcccccgc ggtcgaggtg gtcagttcag tagcagcgcg tgatgccgcc
     5521 gccgaggtcg tcgccccagg tctggcactc gagagcgggc caaaacaagc cgctctacat
     5581 cgtccatccg caattcgaag gcttcgaggt ccgccaaacc gtcaggcggg gcgcggccgc
     5641 ggtcgcctag ctccgacggg cataaggggg gcgttacgta taattctatg ggccgccgcg
     5701 ataggagact ctccacattg tgccgtttcc gtcctcgctg acgaggagag cgccgtcatg
     5761 cgccgccgcc acgccgacgg gccgtgccca aacggaggag tcgttcacga cgaaacccgt
     5821 catgaagtcc tcgtattcgc cggtcggaac accatcgttc aggcgcaccc ggatcacttt
     5881 gtagccggtg cgtttcgcgc ggttccaaga gccgtgctcg gccgcaaata catccccgct
     5941 gtagtccgag ggaaactggt cgccatcgta gatgatcatc cccatcgagg ccgaatgcgc
     6001 ctggatcagc acgtcgggta cggtgatctt gtccttcagg tctggccgtt cgccccgatg
     6061 tcgcggatct tcgttggccc cgagatagta ccagggccag ccgtagaaac cgccgccacg
     6121 cacgcgcgta acaaagtcgg ggacgagatc gtcgccgaga tcgtcgcgct cgttggtcga
     6181 gcaccagaca tctcccgtca tcgggtgcac ggcgagccca acgcagtttc gcagcccgct
     6241 tgcgaacacg cgacgatgtc cgccctccgg gtcgaacacg agcacggccg cgcgatcggt
     6301 ctcgttgccc cacgcgctac ccagaccatg ctccgcttcc caacgcgcga tcgcggcggc
     6361 attgcgcttc cccatcccct ccccgtcatt cgacgccgaa ccgaccgaca cgaacatctg
     6421 acggccatcc ttggagaaaa caatgtcgcg cgtaatgtgg ccgcgctgca ctgagcggcc
     6481 acccgcatgg ggaagctcgc gcacgatcac ctcggctttc ccgcgcgcct tgaggtcgcc
     6541 gctgcgatag ggatagcgga cgacggatcc cgtgttggcg acgtatatcc agtgcggatc
     6601 cgatccgggc ggatagaacg ctatgccgaa cggctgatcg aggccggaga cgaacacgtc
     6661 gttgttgctg ggcttgtccg ctccatcggc ggcgcgcagc acccgaattc gtcccggttc
     6721 cgattcggcg atgaagatgt cgccgttcgg cgcgatccga accacgcgcg gatcgttgag
     6781 attggaggcg aacagctcga cctggaaacc gggcggcacc ttcggcgcgg cggaagccgg
     6841 tttcccgatc acccggggaa aattcgatgc cgaacgggtg gcgtatggcg ggggcagcgc
     6901 ggtgatcttg cgccgaacca agggagcgtc ggtgcgccaa tcaccgaatg ccgccttgtc
     6961 gactaatggc tcgcctagtg aatcaggggt gggttgagct aaaacagctg gcgcaaagaa
     7021 cagcgaccaa gtcgcgaatg ccaccgtcac agctgcagcc atggtgccgc ttgggatgct
     7081 ggggcatgcc aactggcgat tgcgatcggc ctccgtgagc gccatggctg tgacgaatcc
     7141 tcttgctgtc tccattatga gcctcggcca agcattatga aaacgctgga cgctgagcgc
     7201 cgcttcgtgc ggcgtcgtag accggcgcct gctcgcggtc gatgacggcg atgccgtcat
     7261 gcgattgccc cagggctctg aatgcggggt gacggcgagc ctccatcaaa gcccgcagcg
     7321 agatcgccga tgcgttgccg ccaacggagc cccaatcagc atgcgacgac tgacgctcgg
     7381 tttgccgcac gccaagcctg ccgtagagag ccggaaagtc cttgggacag acccgtgaat
     7441 cgttctgaat cctggggcgc cgacgaaccc gctcctgtgc tgctgccgcc cacacgatag
     7501 cacacacacg gacgccgaga gcctactgca atgtgggatt tcaatcccct ctatgtctgg
     7561 ttggggtcaa tcgcgtcgca ggcgattcgg ccccgcggtc ggccgatttc cgcagtaccc
     7621 gcgaaagctg gcggcgtgag cgcctcgtcg cctacttctc caccccgatc accttttggc
     7681 gagccgaccc catttctcgt cttccgtctg gtaaatccga gggaagccgg ccggggacga
     7741 tctataatca acgtgcataa agcgcatgtt cccggaacgg cgatatggaa gcgttcattt
     7801 gcactgcctg cggcacccaa tacacgccga gcagtgtcgc gccgcggcaa tgcgtaattt
     7861 gcgaagaaga gcggcagtac gttccaccac gcggacaaac ctggaccaca ctcgcggccc
     7921 taacggctgg acatttcaac tcctaccgcc agtacgagcc tggagtgatc ggcattggaa
     7981 cacagccaca atttgcgatc ggccaacgtg cgctagttgt ttgcacacct aatggaaatg
     8041 tcttgtggga ctgcattgcg atgctggacc cagcgaccat tgccttgatc aacgggctcg
     8101 gtagcctgaa ggctatcgct atctcgcatc cgcacttcta cacgacaatg gtggaatgga
     8161 gccgcgcgtt cggggagatc ccgattcacc tgcatgccga tgaccaccgc tggattatgc
     8221 gccctgatcc ggcgatccaa ttatggaatg gcgagactct gaagctcatg cccgacctga
     8281 cgctgatccg ctgcgggggc cactttcggg gcggcaccgt ccttcattgg gccaagggcg
     8341 ccggaggccg aggagtgttg tgctcatccg acatcgcgac ggtgacgaca gaccgaaaat
     8401 ttctcagctt catgcgcagc tatccgaatt tgatcccgct ctccgcaaaa caggtgactg
     8461 gaattgctgt cgcgttggag ccttttcagt tcgatacgat ctatggccat tatttcgatc
     8521 gtgtcattac gagtggcgga aaacggactt tggaaatttc ggtcaagcgt tatgtcgacg
     8581 caataagcgg cgtgtacgag catgaatgat cgaacacgat gatcgccgct gctcgaccgc
     8641 gctggtcgag gtatcgacgt ggtccttcgg cacatgctag ggaggctgta aatggcgcca
     8701 agaaagaaag ccaaaggcaa gaccaaggca aagcgcaaga agacctcgcc caccgaggcg
     8761 gcgcacaacc cgctccgccg ggggagcgta tcggcgtcgt gacccactat tacagccacc
     8821 tgtcagtcgc gaccctgcgg gcttgctgat ccgatatcca cgctccgcgt tggctcgcga
     8881 gatccggatt ctcaggatat cgcgaaggcg atgaatgtcg cggacgcacg cgcgcgcatg
     8941 gagcaacagg gcgcggtgcc gctcaccgcc acacctgagc agttcgacgt gatcatcagg
     9001 aatgacgccg agcgcaacgt cagagttctt tgggaggctg gcgtggctgc caattaggtt
     9061 tgggggaaca agccgcattg caacggaggc tgacaatgat cggtgatttg gcgtcacatc
     9121 acggcattgg atcatcagag caaggtccgg caaagaacgt gcaatggacg cgccgcgcct
//
LOCUS       DATLJT010001298         1800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1254534,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001298 DATLJT010000000
VERSION     DATLJT010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254534"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1701
                     /locus_tag="VM910_29835"
     CDS             235..1701
                     /locus_tag="VM910_29835"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI66743.1"
                     /translation="MSPNPDSQREPSREELIAVIAAQAAEIAGLKARIAELERRLGLN
                     SSNSGKPPSSDGLKKPPRPARTQSLRGPSNKPSGGQKGHKGETLEQVAAPDYVIDHVP
                     PSCATCGSAVTLEMTVGHSARQVFDLPGPAPLIVSEHRAHDCRCAACGARTRAAFPDG
                     VNAPVQYGPRIAAFVIYLLHYQLLPEDRLAEAMADLFGVGIVAATIARISRSCAARLQ
                     EFVAIVRDRVAAAAVKHMDETGFRVGGKTQWLHIGSTALLTFYRICHKRGSLLANVVG
                     TVVHDHWKPYYTLPGVRHALCNAHHLRELKALIEIEKEDWARRMQQLLRRACHAANLA
                     RERGGALATLKPRLAEQLERRYDAIVAEGLAYHEAQGALAAPLAHATSKDAGPPRGRI
                     KRRIGHNLLLRLRDRKPDTLRFLRDAAVPFTNNQGERDGRMMKVKMKISGGFRCQDGA
                     ADFACIRSFISTAKKHGWNVIHALTQDPQILISAIRSA"
BASE COUNT          343 a          605 c          545 g          307 t
ORIGIN      
        1 ttcagacgag aactcatatc agcggttgga gaccgactgc atggcactcg aacttcgatg
       61 tgcaaagtga aaattcatcc tctcggcaat gttcggattt acctgtgcca gcgcagaccg
      121 tcctgtggtc ccagaagaat aattgtaact ccctaggttg atcgttgcgt ggcggggtgg
      181 ctgcgccggt agatttttct cgacagcgga atcggctttc gattcaagga ttcgatgagt
      241 ccgaatcctg attcgcagcg cgagccgtcg cgtgaagagc tgattgcggt gatcgcggcg
      301 caagcggcgg agatcgcggg tctgaaggcg cggatcgccg agcttgagcg gcggctcggg
      361 ctgaacagtt cgaacagcgg caagccgccg tcgagcgacg ggctcaaaaa accgccgcgg
      421 cccgcccgga cccaaagcct gcgcggacca tcgaacaagc cgtcgggcgg ccagaaggga
      481 cataagggcg agaccctgga gcaggtcgcc gcgcccgact atgtcatcga ccacgttccg
      541 ccatcgtgcg cgacctgtgg atcggccgtg acgctggaga tgaccgtcgg tcacagtgcc
      601 cgccaggtat tcgatctgcc ggggccggcg ccgcttatcg tcagcgagca tcgcgcccat
      661 gactgcaggt gcgccgcctg tggcgcccgc actcgtgcgg cgtttcccga cggcgtcaac
      721 gccccggtgc aatatggccc gcgcatcgcg gcgttcgtga tctatctgct gcattatcaa
      781 ttgctgcccg aagaccgctt ggccgaggcc atggccgatc tgttcggcgt cgggatcgtt
      841 gctgcgacca tcgcccggat cagccgcagc tgcgccgccc gcctgcagga attcgtcgcc
      901 atcgtgcgcg accgcgtcgc cgccgcggcc gtcaagcaca tggacgagac cggcttccgg
      961 gtcggcggca agacgcaatg gctgcatatc ggctcgaccg cgctcctcac cttctatcgc
     1021 atctgccaca agcgcggcag cctattggcc aacgtcgtcg gcactgtcgt gcacgaccac
     1081 tggaagccct actacaccct gccaggcgtg cggcatgcgc tgtgcaatgc ccatcacctg
     1141 cgcgaactca aggcgctgat cgagatcgag aaggaggact gggcgcgccg gatgcagcaa
     1201 ttgctgcgcc gcgcctgcca cgccgccaat ctcgcgcgcg agcggggcgg tgctctcgcc
     1261 accctcaagc cgcgcctcgc cgaacaactg gagcgccgtt acgacgccat tgtggccgaa
     1321 gggcttgcct accatgaggc ccagggagca ctcgccgcgc cgcttgccca cgccaccagc
     1381 aaagacgcag gccctcctcg cggccgtatc aagcgacgga tcggacacaa tcttctcctg
     1441 cgcctgcgcg accgcaagcc ggatactctg cgcttcctcc gcgatgccgc cgtccccttc
     1501 accaacaatc aaggcgaacg cgacgggcgc atgatgaagg tcaagatgaa aatctccggc
     1561 ggcttccgct gccaggacgg tgccgcagac ttcgcctgca tccgctcttt catctcgacc
     1621 gccaaaaagc acggctggaa cgtcatccac gccctcactc aggacccgca aatcctgatc
     1681 agcgccattc gctccgcata agccgccaat gcccatcctg ggtagttacg aagaaggaat
     1741 tgatcggcaa tttcaagaat ggcgggaccg actatcgacc gaagcgccag ccgcggcatg
//
LOCUS       DATLJT010001299         4969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3781608,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001299 DATLJT010000000
VERSION     DATLJT010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781608"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1628
                     /locus_tag="VM910_29840"
     CDS             354..1628
                     /locus_tag="VM910_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI66744.1"
                     /translation="MARTVRDAALASRTARGKLEPSSQPYYRALEPGLLHLGYRKPRA
                     GAGKWLARQYVGDGVYRLHKIGVADDYSDADGAVILDFKQAQTAARKLLVERAGGIGT
                     VGDAVDAYIHFLEADGRAPRAIQSVRYADRAFIRPALGPIKLAALTTEQLNRWCADLA
                     KRPARIRTKAGAPQRHRHHGDDPDARRARRATAKRIWTVLRAALQHAFDYDKVESDRA
                     WRKVKPFRNVDTARTRYLAVAEAQRLINAADPEFRPLLQGAFLTGARLGQLAALTVSD
                     FGPDVGTLRLRSRKGRGHERTYHVHLSDEGRAFFAHACAGKVGSDLIFTHADGRPWGR
                     AHQLIPIREASIRAKLKPAVNFHVTRHTFASHAVMNGAPLLVVARALGHSSTRMVERV
                     YGHLAPSYEADAIRAAAPQFGIKLGTVRPLGR"
     gene            1631..2533
                     /locus_tag="VM910_29845"
     CDS             1631..2533
                     /locus_tag="VM910_29845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66745.1"
                     /translation="MVRARRGEKLLLVDLLALVAGYTRDEVAAKKFLLDYFDHHHLDI
                     DWDFDVEFEVVPTGGSRPPPPIDALAVELGFWQRYEDSQIDVDWGASSAVRVGPLMWV
                     DYEPHDPYDPDDPIGERHRVIFDLHYQVTLRASLIRINPEPIIAWLHLRGLTPPAAAS
                     SPSASQEKVQLPKSHSRRRRRPPAQVPANDTAAPPRLQSVDNDAPASSDDSKRVPRLK
                     QLHFTGRWQKKFAAWALDQYQPHGDIPETLTAPEMADLFQPWEKREAEKAGKKAPKRS
                     AKDLEALQRRCREFLSKCRDKPAF"
     gene            2678..2947
                     /locus_tag="VM910_29850"
     CDS             2678..2947
                     /locus_tag="VM910_29850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66746.1"
                     /translation="MARREVTGKKPANGDAKKRVRSLPPAFATAFTVTEFCQAHRISE
                     TTYYELKKLGLGPDEMEIGRKRIISIEAAARWRKAREVVAAEAAE"
     gene            3729..4142
                     /locus_tag="VM910_29855"
     CDS             3729..4142
                     /locus_tag="VM910_29855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66747.1"
                     /translation="MASGMRVVSPIPPAESQQSGGSQNAAKSKSDKAYARLSRYELYL
                     FEAKTRAVKNLAKHVAESDPVEAAAALIKATGNIDTAVDLLIAAERSIVSMVPVSKPV
                     VEYDEAIVKETGLAPAPVDLVAETETNGKEQAPRL"
     gene            4431..4655
                     /locus_tag="VM910_29860"
     CDS             4431..4655
                     /locus_tag="VM910_29860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66748.1"
                     /translation="MSNLEHAAELICDPDIREDRRDRLAAEYAAKWAAGELHLEDAEF
                     AKLIRDIGELIALAMWKQHERTMRQLEEAK"
BASE COUNT         1017 a         1512 c         1583 g          857 t
ORIGIN      
        1 ggatcggcca gcgcgcgccg cgcctccgta ccgagcttgt tggcgaccgc gggcggcgtg
       61 ccggccgcga cgaataaacc gctccagagg ccgatattca cctccggata gcctgcctcc
      121 gccatgctcg gcacgtccgg aagttccgag gagcgctgcg atccggtcac cgccaggggc
      181 gcggatcttg ccgccctgca cggttctaac ccacctaaat tcactccgcc gagctccgtc
      241 gggctattgt aaaacgcccg tattaccgat attttttccg ctgaacaccg ccgagctccg
      301 ccgagtttcg ggacgcttat tgcacgcata ttgcacaccg gaggagaaca gccatggccc
      361 gcaccgtccg cgacgccgcg ctcgcaagcc gcaccgcgcg cggcaaactc gagccgagca
      421 gccagcccta ttaccgcgcg ctcgagcccg ggctcctgca tcttggttac cgcaagccgc
      481 gcgccggcgc aggcaagtgg ctggcgcggc aatacgtcgg cgacggtgtc taccgcctgc
      541 acaagatcgg cgtcgccgac gactacagcg atgccgacgg cgccgtcatc ctcgacttca
      601 agcaggccca gaccgccgct cgcaaacttc tggtcgagcg cgccggcggt attggcacgg
      661 tcggtgacgc ggtcgacgcc tacatccact tcctggaggc cgacgggcgt gcgccgcggg
      721 cgatccaaag cgtgcgctac gccgatcgcg ccttcatccg ccctgccctc ggtcccatca
      781 agctcgcggc gctgaccacc gagcagctga accgctggtg cgccgacctt gccaagcggc
      841 cggcccgaat ccgtaccaag gccggcgcgc cccagcgcca tcggcaccat ggtgatgatc
      901 cggatgctcg ccgcgcgcgc cgcgcgactg ccaagcggat ctggacggtt ctgcgcgcgg
      961 cgctccagca tgccttcgac tacgacaagg tcgagagcga tcgggcttgg cggaaggtca
     1021 agccgtttcg caacgtcgac accgcgcgca cccgctacct ggccgtggcg gaggcgcagc
     1081 ggctgatcaa cgccgctgat ccggagttcc gcccgctgct gcagggcgcc ttcctgaccg
     1141 gggcacgcct agggcagctg gcggcgctca cggtctctga cttcggcccc gacgttggca
     1201 cgctcaggtt acgcagccgc aaggggcggg gccacgagcg gacctaccac gtccacctct
     1261 ccgacgaggg gcgggcgttc ttcgcccatg cctgcgccgg caaggtcggg tccgatctga
     1321 tcttcaccca cgccgacggc cggccgtggg gacgggcgca ccagctgatc ccgatccggg
     1381 aggcgtcgat ccgggccaag ctcaagccgg cggtcaactt ccatgtcacc cgccatacct
     1441 tcgcgtcgca cgcggtcatg aacggggcgc cgctgctcgt ggtcgcgcgt gcgcttggcc
     1501 actccagcac gcgcatggtg gagcgggtct acgggcacct cgccccgagc tatgaggccg
     1561 atgccatccg cgccgcggcg ccgcagttcg ggattaagct ggggactgtt cggccgttgg
     1621 ggcggtgact gtggtgcggg cgaggcgtgg ggaaaagctg ctactcgtcg atttgttggc
     1681 tctcgttgcg ggctacaccc gcgatgaagt ggcggcgaaa aagttcttgc tcgattattt
     1741 cgaccaccac catctcgata tcgattggga cttcgatgtg gagtttgagg tagtgccgac
     1801 tggagggagc cgcccgcccc cgcccatcga tgcgttggct gtcgagctgg gtttttggca
     1861 gcgctacgag gattcgcaga ttgatgtgga ttggggggcc agtagcgccg tccgtgtcgg
     1921 gccattgatg tgggtcgatt acgagccaca cgacccgtac gatccagacg acccgatcgg
     1981 cgaacgccat cgcgtgatct ttgacctgca ctatcaggtc acgctgcgag cgagcctgat
     2041 ccggattaat cctgagccta tcatcgcatg gctgcacctg cgggggctga cgccgcctgc
     2101 ggcagcttcg tcgccatcgg cgtcacagga aaaggtgcag ctgccaaaat ctcattccag
     2161 gcgcaggcga cggccgccgg cacaagtgcc tgccaatgac acggctgcgc cgccgagatt
     2221 gcagtcggtc gacaatgacg cgccagcatc ttcggatgac agcaaacgcg tgccgcggct
     2281 taagcagtta cactttacgg ggcgctggca aaagaagttc gcagcttggg cgctcgatca
     2341 gtaccagccc catggcgaca taccagagac gctgacggcg ccagagatgg ccgatttgtt
     2401 tcagccgtgg gaaaaacgtg aggcagagaa agccgggaag aaggcaccga agcgcagtgc
     2461 taaagatctt gaagccttac aacgtcgctg tcgcgagttc ttgtcgaagt gccgtgataa
     2521 acccgcattc taattgcatt tcttgaacgt tcgtccggaa aacgtccgaa acgtccggaa
     2581 aacatccgca gatcgccaac gttcgtccgc agttaatcgt tgcgcgtgta gttgaacgcg
     2641 atcaactata ttcggcgaac gctgacggag gactacgatg gcacgccgtg aagttaccgg
     2701 caaaaagccg gccaatggcg acgccaagaa gcgggtccgc agtttaccac cggcgtttgc
     2761 cacggccttc accgttaccg agttttgcca agcacaccgt atctccgaga caacctatta
     2821 cgaactcaaa aagctcggcc taggtccaga cgagatggag atcggtcgca agcgcatcat
     2881 ctcaattgaa gccgcggcgc gttggcggaa agcacgagaa gtggttgcgg ctgaagccgc
     2941 ggaataactg aattcgacct tttgggacgt atctgcgcgg gcgcggcgat ggctgtgttc
     3001 gcgaatagac gagactttgt ctcgggggcg agcaaatccc ccggagaaag tgcaaatacc
     3061 cggcgcgagg cttccgcggt atgtccgcga cgacgggcta ggaacggccg acgagcggaa
     3121 cgagggagtc cgacaggccg cgacatcggg cgaccagcac cagattggtg ccgcgcggga
     3181 gtcgcaaacc cgtgtcgttt gggaagggtg agtgtcacca taatcgggcc cgcgcgccgc
     3241 agaaggagcg cacaggcggg gaagtcccgg acacaatgac gaattggggc accctgctcg
     3301 cggattgcgg cggcgtgctc cgtttagaaa ttgagagcgc gggccgcggc tgggggctgc
     3361 aaacccgcgc ggaaggggcg agcaagtgcc cgatatataa gatttcattg aaaattcaag
     3421 ctgtttcgcg aacggtatcg tccatctcga agaaggcggc aagctgcgcg gcgttcacgc
     3481 cattaccctg aagaaggacg ccgtgaaaaa gggcgaactg ctcaagcttg gtgagcgggc
     3541 cgtcggtgta cgtcggaggt ttgccttaat gatcccgccc caggaggggc gcggtgtcgg
     3601 ggttggtccg cggcgacgcc gagcagtgtc gccgagatag ctaaaaattt tcgagagttg
     3661 cttcggccga cggcaacccg tcgcgcggcc ctggtcgaag tctgtctgtg aagtcgtagg
     3721 cgcggcaggt ggccagcggg atgcgagtgg ttagtccgat cccgccagcc gaatcgcagc
     3781 aaagtggagg atcccaaaat gctgcaaagt caaagagtga caaagcctat gcaaggttgt
     3841 ctcgttacga gctttacctg ttcgaggcca agaccagagc cgtcaagaac ttggccaagc
     3901 acgtcgcgga gagcgatccg gtcgaggcgg cggccgcgct gatcaaagcg accggcaata
     3961 tcgacaccgc ggtcgacctg ttgatcgcgg ccgagcgctc gatcgtgtcg atggtgccgg
     4021 tctccaagcc ggtcgtcgaa tacgacgagg cgatcgtcaa ggaaacaggc ttggcccccg
     4081 cgccggtgga tctggtggcg gagactgaaa ctaacggtaa agaacaagcc cctcggcttt
     4141 gacatcgacg acgacgattg gattgtcaaa cacggacgcg ggcggaagca gcgcaccatc
     4201 taccgaactt cgagcggaaa atttttcctg cgctacgccg acgaagttga ggttgaggcg
     4261 atctctgaat tggaggcacg ccagctagta agtgcgtggg agttcatcga cgaactcgta
     4321 agtgccttcc gtactggaac acctccaaac atcgaaaaat atccgggctg aaaccagggc
     4381 agcgctgtcc gcgcgaatgc ggcggcgccc tccaatagga gcaagacatc atgagcaatc
     4441 tcgaacacgc cgccgagctc atctgcgatc cagatattcg cgaagaccga cgcgaccgat
     4501 tggcggctga atatgccgcc aagtgggcgg cgggagaact tcacctcgag gacgccgagt
     4561 tcgccaaact cattcgcgac atcggcgagc tgatcgcgct ggcaatgtgg aagcagcacg
     4621 agcgaaccat gcgacagctc gaagaagcga agtaactgtc ttccagaagc gcggctgtct
     4681 ccaaccgggg agcggcggcc tcggcaaagg cgacctgcgg aatgcgcttc tggagaatgg
     4741 acatccgttg aaaaggtcgc caaccgccgc aatagcttta aggaaaagca aaatgctaat
     4801 cgatgatttc tggagttcag tcgatcggcg cgaacaaagc aaatgctggg tttggacccg
     4861 aggcaaactg ccggagggtt acggtcagct acgcgtccac ggtaggaata ttctagcaca
     4921 tcgtcatgca tgggaattga ccaacggtcc tatcccaact tatcgctta
//
LOCUS       DATLJT010001300         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3781857,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001300 DATLJT010000000
VERSION     DATLJT010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3781857"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..385
                     /locus_tag="VM910_29865"
     CDS             56..385
                     /locus_tag="VM910_29865"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HVI66749.1"
                     /translation="MSRSTEAQKAARLNAAHGLLTRGQSVAEAALSLSRQFAMSRRQA
                     YRYIEEAQMLDHPVTVAEPAAAVTFKLPPSLVDAIRARAAAEGTTISDMVSRALRAFL
                     AEADGNG"
     gene            529..1647
                     /locus_tag="VM910_29870"
                     /pseudo
     CDS             529..1647
                     /locus_tag="VM910_29870"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2210..2914
                     /locus_tag="VM910_29875"
     CDS             2210..2914
                     /locus_tag="VM910_29875"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HVI66750.1"
                     /translation="MLDGLDKELEKRGHRFVRYADDCNIYVRSQRAGERVMAGIEKFL
                     AKRLRLKVNKAKSAVAKPSVRKFLGFSFTSGKEPRRRIAPQALDRFKARVRGLTRRTR
                     GQSLAQTVKGLSVYLIGWRGYFGFCQTPSVLRALDEWTRRRLRAIAWKQWKRGRTRFA
                     ELRRCGVGRDLAAQTAGSPHGPWRLANSPALTIAMPNRFFASLGLASVGAPPPHNPPN
                     RRIRTRTSGGVGGEEP"
     gene            complement(2924..3124)
                     /locus_tag="VM910_29880"
     CDS             complement(2924..3124)
                     /locus_tag="VM910_29880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66751.1"
                     /translation="MPVYTDGAALSEARLEKIVAYLSAYEIEQARRRGESDPMTTVKS
                     TRFLDGRTTIQIDIAPREQQSG"
BASE COUNT          613 a         1005 c         1037 g          552 t
ORIGIN      
        1 atcgttctcg ttctgtgctt gatttgcgtc tcaaaaggta atatgcacat gacacatgtc
       61 gcgatcgacc gaagcgcaga aggcggcgcg tctcaatgca gcccatgggt tgctcacgcg
      121 ggggcaaagc gtggcggagg ctgcgttgtc gttgtcgcgc cagttcgcga tgtcgcgtcg
      181 tcaggcctat cgctacatcg aggaggccca gatgctcgac cacccggtga cggttgcaga
      241 gccggcggcg gcggtgacgt tcaagctccc cccgagcctg gtcgatgcca tccgggcgcg
      301 ggcagcggcg gaagggacga cgatcagcga catggtgagc cgggcgctgc gggcgtttct
      361 cgccgaagcc gacggcaatg gctgagcgac gcgggcatga tcgcagcgtc cagttcgagc
      421 atgccttcga tcgtctcctc gtcgtcaaac tggagcaggc ctacgagatt ttgggaccga
      481 agcaggtccg tgccatcggc tccgatccgc gcgtgagagg gaatagcgat gaaggccgcc
      541 gcgatctacg ccagagtatc ctccgagcag cagaaggagg agaacacgat cgccagccag
      601 acggcggcgc tgatcgagtt cgcccgggag caaggataca gcgtccccga tgaatgggtg
      661 atcgaagatg aagggttcag cggcgccagc ctgcagcggc cgggcttgga gcgactgcgc
      721 gatctcgccg ccgagggcca cattcaggct gtcctcatcc attcgcctga ccggctgagc
      781 cggaagtatg cctatcaggt cctgctgacc gaggagtttg cccggcaggg cgtcgagacg
      841 gttttcctga aggcgccgca ttccggcacc ccggaagatc agttgatgct gcagttccag
      901 ggcatgatcg ccgaatacga gcgggcgcaa attctcgaac ggtcccgccg cggcaagcgt
      961 catcgcgcca aagccgggga agtatcggtc ctgtgcggcg cgccctatgg ctaccgctat
     1021 attcgcaaga ccagcgatac gccggcacgc tacgagatcg acgccgccga agccgaggtc
     1081 gtgcgattgg tgtacgacaa atacacggtc gatggtttga gcattggcgc gattgcgcgg
     1141 ctgttgcggg agctggggct gcccacgcgc cagcgggtaa cccgctggga gcgatcggtc
     1201 atatgggcga tcctacgcaa tccggcctat cagggcacgg cctgcttcaa caagacaggg
     1261 atcggcccca ggcagaaagt gacgaggccg ttccgcctat cgggacgggc ggtccatggc
     1321 gagaagagca gcgggcacga gcgcccgcgc gacgaatgga tcgagatacc agttccagcc
     1381 atcgtcagcg aagagacttt cgccttggcg gcggagcgcc tggccgacaa caagcgcttc
     1441 gcgccccgcc ggaccatcga gcccagcatc gtccagggac tggtctcctg ccacaaatgc
     1501 ggatacgcgc tgtcgcggac gtcgacccgc agctcggcgc gcaaaatcca ttattatcgc
     1561 tgtctaggct cggatgcctg gcgccacctt cgtggtccgg tatgcgacag ccgtccgatc
     1621 cgccaggatc tgctcgacca gatcgtctgg caggaggtca tccgcctgat cgaggatcct
     1681 acgctggtcc aggccgaact cgaccggcgc cttgatgcag cgcgcgcggc agaaccgacg
     1741 aggcgtcgtc aagacgctct tgaacgcgag ctggcgcggg tcagcaagag catggagcgt
     1801 ctgatcacag cctaccagga ggatctccta tcccttgatg aactgcgccg tcgaatgccg
     1861 gaactgcgcg ctcgcgagca gtccatgcgg tcggaattgc aggccatcct cgatcaggca
     1921 gcagaccgga tgtcgttcct gcgtctggct gaaaccctaa ccgcgttcct caagcggctg
     1981 cacgaatcgg cggaatcgtt ggaaatcact gagcggcaga aagtggtccg actccttgtc
     2041 aaagatgtct tggtcgacga cgacaccatc acgatccggc actcaatccc gtccttgccg
     2101 aggacaccac ccaccaggac tgagccgcca ccagacggca aatcgcggac ggatgaccaa
     2161 agttaccttt tgcgttcgag gagtgctctc gccgctactg tcgaacctga tgctggatgg
     2221 gctggacaag gagctggaga agcgcggcca tcgcttcgta cgctacgccg acgactgcaa
     2281 catctatgtg cgcagtcagc gggcaggcga gcgggtgatg gcaggcatcg agaagttcct
     2341 ggccaagcgc ctcaggctga aggtcaacaa ggccaagagc gcggttgcaa agccgagtgt
     2401 ccgcaagttc ctgggcttca gctttacgag tggaaaggag ccgcgccggc gcattgcgcc
     2461 gcaggcgctt gaccgcttca aggcgagggt tcgggggcta acccgacgca cgcgcgggca
     2521 aagcctcgcg cagaccgtca aagggctgtc cgtctacctg atcggatggc gcggctactt
     2581 cggcttctgc cagaccccgt cggtattgcg cgcgcttgat gaatggacca ggcgacggtt
     2641 gcgcgccatc gcctggaagc aatggaaacg ggggcgtact cgctttgctg agctgcgacg
     2701 ctgcggcgtc ggccgggacc tagcggcgca aaccgccggc agcccacacg gcccttggcg
     2761 gctcgcaaac agccccgcgc tgaccattgc tatgccaaac cgcttcttcg cctctctcgg
     2821 cctggcttct gtcggcgccc cgccaccgca taatccaccg aaccgccgga tacggacccg
     2881 tacgtccggt ggtgtgggag gggaggagcc gtgaggttcc cccctatccc gattgctgct
     2941 cgcgcggcgc gatgtcgatc tggatcgtgg tgcgaccgtc caagaagcga gtcgacttca
     3001 ccgtcgtcat cggatcggac tcgccgcgcc ggcgggcctg ctcgatctcg taggccgaga
     3061 gataggcgac gatcttctcc agacgcgcct cagagagtgc tgcaccatct gtgtatacgg
     3121 gcatccgcat aacctccaag tgcgcgtcgc aagtacctcc cgaacgcgcc ctacggttgc
     3181 tcatcttgga ccatacttca cggcgtc
//
LOCUS       DATLJT010001301         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_566031,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001301 DATLJT010000000
VERSION     DATLJT010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566031"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VM910_29885"
     CDS             complement(<1..294)
                     /locus_tag="VM910_29885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66752.1"
                     /translation="MTVTIGRRELLAALGGAAAAWPLAGRAQQAERVRRIGVLMNLAA
                     DDPAGQVRLAAFLQGLQQLGWTDGRNVRIDARWAAGDPDHFRKYAAELVALGPD"
     gene            complement(530..1501)
                     /locus_tag="VM910_29890"
     CDS             complement(530..1501)
                     /locus_tag="VM910_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66753.1"
                     /translation="MKLPRRKFLHLAAGAAALPAVSRIARAQAYPTRPVRMIVGYPPG
                     GTADVVARLISQQLSERLGQPFVVENRAGAGTNIATEAIVKSAPDGYTLLVVDASPAI
                     NTTLYHNLNFFFFRDIAPVACVISVPLFMVVNPAVPAKTVPEFIAYAKANPRRVNMAS
                     NGNGGLLHLAGELFKMMTGVDLVHVPYRGAQPALTDLISGQVQVMFVASTVEYIRTGK
                     VRALAVTTPTRSEVLPDVPPLSEFVPGYDAAAWIGVGAPKNTPPEIIDKLNKEVNAAL
                     ADPKLKLRLADLGSSAAGGSAADYARRLQEEAEKWGKVIRAAHIKAE"
     gene            complement(1526..2506)
                     /locus_tag="VM910_29895"
     CDS             complement(1526..2506)
                     /locus_tag="VM910_29895"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66754.1"
                     /translation="MRRREFIALLSSGVTGWPLAARAQRPNVMRRVGVLTGAFGPDSQ
                     ARVAVFLQALAQLGWMEGQNVRLEIRQGGGDFDTIRKHAAELAALGPDVILAVGGSAT
                     GLLLQATRTVPIVFAIVPDPVGSGFVESLSRPGGNATGFIQFEYGLSGKWLELLKEIT
                     PGVTQVAVLREHTPAGVGQFAAIQAVAPSLGVEVRPINLQNAAEIERDLVSFARVSNA
                     GLIVASSTTALVNRNLIIALAMRHKSPAVYAQREFVVAGGLMSYAADFSVQFRNAAGY
                     VDRILKGEKPADLPVQAPTKYALVINLKTAKTLGLELPASVLARADEVIE"
     gene            2755..>2970
                     /locus_tag="VM910_29900"
     CDS             2755..>2970
                     /locus_tag="VM910_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVI66755.1"
                     /translation="MEEHQIGWIAAIIIGGIAGWLAEQFMKSQMGLLMNIVLGIIGAA
                     VASWLLGFLGITLAGWIGYLIAGFIGAC"
BASE COUNT          589 a          901 c          894 g          586 t
ORIGIN      
        1 gtcaggtccg agcgccacca attccgccgc gtatttgcga aaatggtcgg gatcgcccgc
       61 tgcccaacgc gcatcgatcc gcacattgcg gccgtcagtc cagcccaatt gctgtagtcc
      121 ttgcaggaac gccgcaagtc ggacctgtcc tgccggatcg tcggcggcga ggttcatgag
      181 cacgccgatg cgccgcaccc gctcggcttg ctgcgcgcgc cccgcgagcg gccacgcggc
      241 cgccgcgccg ccgagcgcgg ccagtaattc ccgccgtccg atggtgactg tcatacgccc
      301 ctcccgcgcg aggtgcgtga agggaaagat accacgctat gatggtgcag tcttcacgtt
      361 caggggggca ggatgctggt tgcttccacc ctaaggcaga cattcggact gaggctgaga
      421 ctcaatcaac cgacagcgag gaccggccgt tttattcgat taccctagtc gcaacatccg
      481 cgcttcgcct aacgaaaggt tatggaatgt cggcgcgacg atgacggccc tactcggcct
      541 tgatgtgggc cgcccggatc actttgcccc acttttcggc ttcctcctga agccgcctcg
      601 cgtaatccgc ggccgagccg ccggcggcgc tgctgcccaa gtcggcaagc cgcaacttca
      661 atttggggtc cgccagcgcg gcgttaacct ccttgttcag tttgtcgatg atctcagggg
      721 gcgtgttttt gggtgcgccg acacctatcc atgcagccgc atcgtaaccc ggcacaaact
      781 cactcaacgg tgggacatcc ggcaggacct ccgagcgcgt cggagtggtg accgccaatg
      841 cgcgcacttt gccggtcctg atgtactcaa cggtcgaagc gacgaacatc acctgcactt
      901 ggccacttat gaggtcggta agcgcgggct gagcgccgcg ataggggaca tgtactaggt
      961 cgacgccggt catcatcttg aatagctcgc cggccagatg gaggaggccc ccattgccat
     1021 ttgacgccat gttgaccctg cggggattgg ctttggcgta agcaatgaac tcggggactg
     1081 tcttcgctgg aaccgctgga ttgaccacca tgaaaagcgg gacgctaata acgcaagcaa
     1141 ctggcgcgat gtcgcggaaa aaaaagaaat taaggttgtg gtacagcgtc gtgttgatcg
     1201 caggcgaagc gtcgaccacg aggagcgtgt acccgtctgg cgccgacttc acgattgcct
     1261 ctgtagcgat gttggtgccg gcgcccgctc tgttctcgac gacgaaaggt tggccgaggc
     1321 gctccgagag ctgttgactg attaagcgcg cgacgacgtc ggcagtgccg ccggggggat
     1381 agccaacgat catgcgcacc ggccgcgtcg gataggcttg cgcccgcgcg atgcgcgaga
     1441 cggctggcag cgcggcagca cccgcagcca gatgcaagaa tttgcggcgg ggaagtttca
     1501 ttttgaaact ccctctctgc tcacatcact cgatcacctc gtcggcgcgg gcgagcacgg
     1561 acgccggcag ttcgaggccg agcgtcttgg cggtcttcag attgatcacc agcgcgtatt
     1621 tggtcggcgc ctgaaccggc agatcagccg gtttttcgcc tttcaatata cgatcgacgt
     1681 agcccgcggc attgcggaac tgaacagaaa agtccgcggc atacgacatc aggcccccgg
     1741 ctacgacgaa ctcgcgctga gcgtaaactg ctggcgattt atgccgcatg gcaagcgcaa
     1801 taatcagatt gcgattaacc aatgcggtcg tgctcgaagc cacgatcagc ccggcgttgc
     1861 taacgcgtgc aaaggacacg agatcgcgct caatttcggc ggcattctgg aggtttatcg
     1921 gtctcacctc gaccccgagc gatggtgcga cggcttggat tgctgcgaat tggcctaccc
     1981 cggcgggggt atgttcccga agaaccgcca cctgcgtcac gcccggcgta atttctttga
     2041 gtagctctag ccattttccg ctcaagccgt attcgaattg gatgaaaccg gtggcgttgc
     2101 cgcctggccg cgacaggctt tcaacgaaac ccgagccaac tggatcgggc acgattgcaa
     2161 atacaatcgg caccgtgcga gtcgcctgaa gcaatagccc agtagccgag ccgccgacag
     2221 ccaggatgac gtccgggcca agtgccgcca attcggccgc atgcttgcga atggtatcga
     2281 aatcgccgcc gccctgacga atttcgagcc gcacattttg gccctccatc cagcccaatt
     2341 gcgccagcgc ctgcaggaat accgcgaccc ttgcctgcga gtccggacca aaagcgcctg
     2401 taagcacccc aacgcgccgc atgacgtttg gccgctgagc ccgcgcggcg agcggccatc
     2461 cggttacacc gctgctgaga agtgcgatga attcccgccg cctcatgggc ttacccgaag
     2521 gccaaggatt gcggaccaag tatagcaggt cctagagccg gttcaggctg tgcatcgcag
     2581 caaaaggcgg ccactgatgt ccgctttggg tcaaaagcag acgtcccgcc ctacttggac
     2641 tttgtccgct ttacccccga aagcggacat ggatcggcgt tcgagggctt ttgcgcccca
     2701 tgcggattag taatatggtt tcggaaactt agtcgcgctt aggaggaaac ggcaatggaa
     2761 gaacaccaga tcggctggat cgcagccatc atcattggcg gaatcgccgg ctggctcgcc
     2821 gagcagttta tgaaaagcca aatgggactt ctgatgaaca ttgtgcttgg catcataggg
     2881 gccgctgttg ctagttggct acttggcttc ctcggaatca cattggccgg ttggatcggt
     2941 tatttgattg ctggcttcat cggagcttgc
//
LOCUS       DATLJT010001302         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_334879,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001302 DATLJT010000000
VERSION     DATLJT010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334879"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VM910_29905"
     CDS             complement(<1..286)
                     /locus_tag="VM910_29905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66756.1"
                     /translation="MRVLMLPAVAAILLIGADLLCAPAANAQVRSPSPGATVQPSNIP
                     DQKLNAAAAAVQRVTSLKKDYQQRITAAAPADKERIVNEANSALEKAVTDQ"
     gene            complement(692..1663)
                     /locus_tag="VM910_29910"
     CDS             complement(692..1663)
                     /locus_tag="VM910_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66757.1"
                     /translation="MKRRTFLYLSAAAAALRVAPSSAWAQAYPARPVRLIAPFPPGGV
                     VDLYARLIGQWLSEHLGQPFVIENRAGAGGNVGTEVVVHAPPDGYTLLQLSSSNAWNA
                     TLYDNLKFDFIRDIAPVASIYLAPAVLVVHPSFPVKSVPELIAYAKANPGRINMASGG
                     VGSAQHVYGELFKMMAGVDMLHVPYRGGGPALADLLAGQVPLMFDTLATSIEHIRAGK
                     LRALAVTGAVRSQVLPDTPTVGDFVPGYEGIGWQGVGAPRNTPAEIIDKLNKEINAGL
                     GDPRIKARVADLGGTVFISSPADFGAFIAAYTEKWAKVIKFSGAKIY"
     gene            1855..>3909
                     /locus_tag="VM910_29915"
     CDS             1855..>3909
                     /locus_tag="VM910_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI66758.1"
                     /translation="MHILSFGDAFHAGRLVKLLTIAALAVPVALECPGATAWAQTAEK
                     RADPAIRPEFREPVTLASKEGVLEVRLTARQGQAALDTAAKPVQNFLLFDYEVIRGTA
                     SDGRSSGGNLYPAPTLHVFPGETLIVHFENGLSGLTIRDYFSPQYTPKGQSIPIYPQQ
                     MTSSPINLHTHGAHISPRGNADNVLLHIPPGMSNTYTYDIPRNMPQGLYWYHSHLHGL
                     TSAHVYTGLVGLLAIGRTDGNLPLVTEKNIPIRNMALQYNFVFDRAGGLAQLNNPSWP
                     QWVSTITPPKPGELANGTYQPLLAPVNFKQSKPGTKYFTVWYAGPLSIHNRRGSLQFI
                     PSNLQHFVAAGGKAESDAPADPSLPDYQRDVQFTVNGQFQPVIRSKAGQTEIWVLANV
                     SDFAYMNVQLTETATGRHPPIAIVGQDGNPYTSVHYPPTDQGMRLLIPPASRFAIAVT
                     IPSEGDLVLEMPERGGGAKTITEPGVLYTNNETDNPPAVLGSLSVLPSAVSYADGFFV
                     FPTQVLARATASEGGGVTTPFVEGQPLGGYTSFVDLANTTPDVKRQILISGEFLNNLA
                     STEDPKAFVYAFDGGAFPNVPLIQPRLNSVEEWRFVNHNNDEHPIHVHVNDFQVIEYF
                     DPTTGLRTGPDKFSIDNANAPAPTMHSDESVIQPGILAIRTRFDEYTGLYVMHCHRLN
                     HED"
BASE COUNT          794 a         1227 c         1147 g          741 t
ORIGIN      
        1 cttggtcggt aactgccttt tccagcgcgc tattggcctc gttcacgatg cgctccttgt
       61 cggcgggggc cgccgcggta atccgctgtt gatagtcctt cttgagactg gtcacccgct
      121 ggacggcggc tgctgccgca ttgagctttt gatccggaat attcgacggc tggactgtgg
      181 cgcccggcga aggtgatcgg acctgagcgt tcgctgcggg agcgcagaga aggtccgcac
      241 caatcagcag gatcgcagcg acggcaggca gcatcaagac tcgcatcatc gcctcctttg
      301 caagacatcg cgggaacgcg ggatcgaatg tcaagtactt ggaagattgg ctaaaaaacc
      361 gccgggagtg aacttgcgct tttgttaaag cgaggtagtc ggcacaagga agcggagtgc
      421 tggcggcaat atcttggcaa tgagcttggc gaacgcggcc ggcgccgcag ggtcggagag
      481 atattgttgt agttgccgcg ttgccccgcc cgagcccacg ccttgctacc aggctttgct
      541 ttgccttgcc ctgcgctgtg gcgcgaaccc gtgcaccgca gcaaacgacg tgcgcgggct
      601 gtaaacatct actcggtcac ctcgtcgcgc gaccaaacgc cggcgatttc gcggaacgga
      661 acgttatgga atgtcgagcg ggtaaccctt gtcagtagat tttggcgccg gagaacttga
      721 tcaccttggc ccacttctcg gtatatgcgg cgatgaaggc gccaaaatcg gcaggcgagc
      781 ttatgaacac cgtgccgccg aggtcggcaa cccgtgcttt tattcgggga tcgccgaggc
      841 ccgcattaat ctccttgttg agcttgtcga tgatctcagc gggcgtgttt ctaggagcgc
      901 cgaccccctg ccagccgatt ccctcgtaac ctggtacaaa gtcacctaca gtcggggtgt
      961 ccggcaacac ttgtgagcgc accgcgccag tcaccgccag ggcgcgcagt ttgcctgctc
     1021 tgatgtgctc gattgacgta gcgagcgtgt cgaacatgag tggcacctgc ccggcgagaa
     1081 ggtcagcaag tgccggccct ccgccgcgat acggcacatg gagcatgtcc acaccggcca
     1141 tcatcttaaa cagctcgcca taaacgtgtt gcgcactccc gacgccgccc gaggccatgt
     1201 tgatcctgcc cggattggcc ttggcgtacg cgatcagctc aggaacggac ttcaccggga
     1261 atgacgggtg taccaccaag acagctggcg cgaggtagat gctcgcgacc ggcgcaatgt
     1321 cgcggatgaa atcgaatttg aggttgtcgt agagtgttgc gttccaggcg ttagaagaag
     1381 agagttggag gagcgtgtag ccgtctggag gcgcatgcac gaccacttcg gtcccgacat
     1441 ttccgcctgc gccagcccga ttctcaatga caaatggttg accgaggtgc tcggataacc
     1501 attgaccgat taggcgagcg tacaggtcca ctacgccgcc cggcggaaac ggtgcgatca
     1561 accgcaccgg ccgcgccgga taggcttgcg cccatgcgct cgacggagcg acgcgcaggg
     1621 ccgcggcggc ggcgctgaga tagagaaagg ttcggcgttt catctcgcgc tctccaggcg
     1681 acatggagcg cgcaggctag cgcatttcgc gacggccgcg atatcggttt gatgcggcgg
     1741 cgcggttcac ggcccctgtt cggccgcttt gatccggtaa ttgctgcatg tcgggcgctg
     1801 cagcaggttt tcggttctgc tacaatttaa aatcgcaaag tcctagggcc cctcatgcac
     1861 atactgtcat tcggcgacgc gttccacgca ggtcgcctgg ttaagttgct gacgatcgct
     1921 gccctcgctg tcccggtggc gcttgaatgt cccggcgcga ctgcctgggc gcaaactgcc
     1981 gagaaaagag cagatccggc cattcgaccg gagttccggg agcctgtaac gcttgcgagc
     2041 aaggaaggcg tgctcgaagt ccgcctcacc gcgcggcaag gccaagccgc cctcgatacg
     2101 gcagccaagc ccgtccaaaa ctttcttctt ttcgactatg aggtgatccg cggcacggcg
     2161 tccgacgggc ggagctccgg cggcaatctc tatccggcgc ccactctgca cgtatttccc
     2221 ggcgaaacgc tgatcgtcca tttcgagaac ggcctcagcg gcctgaccat ccgggactac
     2281 ttcagtccgc agtacacccc aaagggtcag tccattccga tctacccgca gcagatgact
     2341 tcctcaccga tcaatctcca cacccacgga gctcatatca gtccgagggg aaacgccgac
     2401 aacgtgttgc tgcacatccc tccaggcatg tccaacacct acacctacga cattccaagg
     2461 aacatgccgc agggactgta ttggtatcac agtcatcttc atgggctcac gtcggcccac
     2521 gtctatacag gtctcgtcgg actgctggcg atcggacgaa cggacggcaa cctgccgctc
     2581 gtcacggaga agaacattcc catccgcaac atggcgcttc agtacaactt cgtctttgac
     2641 cgtgccggcg gtctagcgca actcaacaat ccgagctggc cccagtgggt cagcacgata
     2701 accccgccca agcccggcga gctggcgaac ggaacttatc agccattgct cgcgccggta
     2761 aacttcaagc aatcgaagcc gggaacgaag tactttacgg tctggtacgc ggggccgctt
     2821 tcaatccaca atcgccgcgg gagcctccag ttcatcccca gcaatctgca gcacttcgtc
     2881 gcggcgggcg gcaaagccga gagcgatgcg ccggcggatc catcgctgcc ggattaccag
     2941 cgggatgtgc aattcacagt caacggccag tttcagccgg tcatcaggag caaggccgga
     3001 cagaccgaga tctgggtgct cgcgaatgtg agcgactttg cctacatgaa cgtccagctc
     3061 acggaaaccg caacggggcg gcacccgccg atcgccatcg ttggccagga tggaaacccc
     3121 tataccagcg tccattatcc gccgaccgac cagggcatgc ggctgctcat tccaccggcg
     3181 agccgattcg cgattgcagt gacgataccc tcggaaggcg atctcgttct cgagatgccg
     3241 gagcgcggag gcggcgcaaa gacgatcact gagcccggcg tgctctacac caacaatgaa
     3301 acggataatc ctccggccgt gctcgggagc ctgagcgtcc tgccgtcagc agtcagctat
     3361 gccgacgggt tcttcgtctt tccaacccag gtgctagcga gagccacggc ctcggaaggg
     3421 ggcggcgtaa cgaccccctt cgtcgaaggt cagccgctcg gcggctacac gtcctttgtc
     3481 gacctcgcca acaccacgcc ggacgtcaag cggcagatcc tgatatccgg tgaatttctc
     3541 aacaatttgg cgagcaccga ggatccgaaa gcgttcgtgt atgcattcga cggcggcgca
     3601 ttccctaacg tgccgctgat ccagccgcgg ctcaactcag tcgaggagtg gaggttcgtc
     3661 aaccacaaca acgacgagca tcccatccac gtccacgtca acgactttca ggtgatcgag
     3721 tactttgatc ccacgacggg tctgcgcacc ggccccgaca agttttccat cgacaatgcc
     3781 aacgcgccgg caccgaccat gcacagtgac gagagcgtga tccaacccgg catccttgcg
     3841 atccgcaccc ggttcgacga atacaccggg ctttacgtga tgcattgcca tcgcctcaac
     3901 cacgaagac
//
LOCUS       DATLJT010001303         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3093072,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001303 DATLJT010000000
VERSION     DATLJT010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093072"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VM910_29920"
     CDS             complement(<1..1503)
                     /locus_tag="VM910_29920"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66759.1"
                     /translation="MSANASDERFNRAVTAHLRQEVQAPAAAVSDFLDIIIEDARRQK
                     LDHALADLERMRSASIQLTTFVKSVTTDGSSARGQAENFEAFQRRLRHDLRSPLNVIK
                     GYSELLIEDMDADNNHPLREDLTKLRDSANQLLAQIDAIVTLTRSDQTPQESRAQQVS
                     CVEDVLRTVQPLQMGSASPETVLSGRVLVVDDNAANRDVLARRLTREGHQVVTTASGA
                     SGLELVATREFDLVLLDLIMPEMNGFDVLRRLKATEHTSHVPVIVISALDELDSVVRC
                     IESGAEDYLTKPFNPVLLRARINASLEKKRLRDRERKFIQDLEQERQRSQSLLLNILP
                     QIVVDRMRDGEKVIADRIIDATILFCDLVGFTALSQELSADRTIAFLSRIFSAFDRLA
                     LEHGVEKIKTIGDAYMVAAGVPGPQADHVLRIVTLAPRMLEMVDVVAKETGLKLQARI
                     GIHTGPIVAGVIGTHKFVYDVWGDTVNTASRMESHSLAGRIQISAATRAAL"
     gene            complement(1560..1934)
                     /locus_tag="VM910_29925"
     CDS             complement(1560..1934)
                     /locus_tag="VM910_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66760.1"
                     /translation="MPRILLVEDNDMNRDMLSRRLIRSGYEVFLAVDGQQGADMALSE
                     QPDLILMDMSLPVIDGWEATRRIKANDATRRIPIIALTAHAMAGDREKAMEVGCEDYD
                     TKPVEMSRLLGKIAALLKSTAG"
     gene            complement(1958..>4580)
                     /locus_tag="VM910_29930"
     CDS             complement(1958..>4580)
                     /locus_tag="VM910_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI66761.1"
                     /translation="AIESLRHLLEVQAEANLLAGLLIESSMVTESVRLQPLRENIDAA
                     RRRIETSLNALADPEQRKRLIDLHERLAAMAGQDGIVDVRARELLRQQEAQLAFAATQ
                     NEAVKLKQAVDRLVEQQGKDAAAVSARAAEQIRSGRILLIALSIVALVAAGLIAWLYV
                     GRNIVSRLAHLSSAMLAIAAGRRESAVPVTGADEIGAMGRAVEVFRRNAVELDQLLAE
                     RADAAIKLEKIVEQRTAELQRRGEVMRVTFENMEHGVLIFDREMKLVAWNRQITKLLE
                     LPETFLAGEPHFSDFIRFQAERGEYGDVDVGAEVQQFTARSAGRYSTERTRPNGTVLE
                     IRHNPLPHGGVVNIYTNITDRKHYENALTAARDQAEAMSRTKSSFLANMSHELRTPLN
                     AIIGYSEILQEDAADKEDKEPIDDLQKIESAGRHLLGLINNILDLSKIEAGKMDIFIE
                     EVDVQALVKEVLSIVKPLADKNENSIEVICPADIGSFRSDHTKVKQCLLNLLSNANKF
                     TSKGTLTLMVAREDNSRASFRVSDTGIGMTEEQLGQLFEAFSQTYASTTRRFGGTGLG
                     LSITKHFCTMLGGNVTVESMPGTGSTFTITLPDYGVVPAAAESPAPAVETADGRATVL
                     VVDDDPMVCNLLAKTLGKEGYRVISARNGIEALALAREHRPQAITLDVLMPQMDGWAA
                     LKELKADAQLRDIPVIMVTVLNERGMAIPLGAADFLAKPVDRQRLTAILREHCASPSS
                     ASILVVEDDLATREMLCRSVVSMGYVAHAAVNGRSGLDWLGNHPAPSLILLDLLMPEL
                     DGFEFLQELRKRPAFVDIPVIVVTAKDLTAEDYRILSGQTERIIAKNQAYLSELAVAV
                     RARLAQQPTREAERLAY"
BASE COUNT          865 a         1423 c         1310 g          982 t
ORIGIN      
        1 aagcgccgcg cgcgttgcgg ctgaaatctg aatccgaccg gcaagcgaat gcgactccat
       61 gcggctcgcg gtattcaccg tatcgcccca cacgtcgtag acgaatttgt gcgtcccgat
      121 cacgcccgca acgatcgggc cggtgtgaat tcctatgcgc gcctgcagct taaggcctgt
      181 ctccttggca acgacatcaa ccatctccag catgcgcggg gcaagcgtca cgatgcgaag
      241 gacgtggtcg gcttgcggcc cgggaacgcc ggccgccacc atgtaggcgt cgccgatggt
      301 ctttatcttt tccaccccgt gttcgagggc aaggcgatcg aaagcggaga atatcctgct
      361 caggaacgcg atggtgcggt cggccgacag ttcctgactg agcgcggtaa acccgacgag
      421 gtcgcaaaac aggattgttg cgtcgatgat tcgatcggca atgacctttt cgccatcccg
      481 catgcgatca accacgatct gcggcaggat gttgagcagc aacgactggg accgttggcg
      541 ttcctgttcc aagtcctgga tgaatttcct ctcgcggtca cgtagccgct ttttctccag
      601 tgaggcattg atgcgcgccc gcagtagcac gggattgaac ggctttgtaa ggtaatcctc
      661 tgcgcccgat tcaatgcaac gcacaacgct gtccaactca tctagcgcgg aaatcacgat
      721 taccggaacg tggctggtgt gctcggttgc cttgagccgt cgcaacacgt caaaaccgtt
      781 catttccggc atgatgagat cgagcagaac caagtcaaat tctcgggtcg cgacaagctc
      841 cagccctgaa gcaccactag cggtggtcac aacttgatgg ccctcgcgcg tgagccggcg
      901 cgcaagcacg tcgcgattgg cggcattgtc gtccacgacc aaaacacgac ctgacaacac
      961 cgtttccggc gacgcgctac ccatttgcag tggctgtacg gtacgcaaca catcctcgac
     1021 acaactgact tgttgcgcac ggctctcttg gggtgtctgg tctgatcgcg tcaaggtgac
     1081 gatcgcatcg atctgagcaa ggagctgatt ggccgagtct cttagcttag taaggtcctc
     1141 ccgaagcgga tgattgttgt ccgcatccat gtcctcaatc agcagctcgc tatagccctt
     1201 gatgacattg agcggcgatc gcaagtcgtg gcgaagtcgg cgctgaaatg cttcgaaatt
     1261 ttcggcttgc cctcgggccg aagagccatc cgtggtcacg ctcttcacga acgtggtcaa
     1321 ctggatgctt gcgctgcgca tgcgctcgag gtccgcaagc gcatgatcaa gcttttgcct
     1381 gcgcgcatcc tcgatgataa tgtcgagaaa atcggaaacg gcagcggctg gagcctgcac
     1441 ctcttgcctt aagtgtgccg tcacggcgcg attgaagcgc tcgtctgatg cattggccga
     1501 caaggggctt tctccctggg ccgcgagact tctccgagaa ccgccaccgt cgctgcggct
     1561 cagcccgcgg tggacttaag tagggcggca atcttgccaa gcagacgcga catttcgact
     1621 ggcttggtgt cgtaatcttc gcagccaact tccatcgcct tttcgcgatc gccggccatc
     1681 gcgtgcgcgg ttagcgcgat gatgggtatc cgccgagtgg catcgttggc cttgatccga
     1741 cgcgttgctt cccaaccgtc gatcaccggc aggctcatgt ccatcaggat gaggtcgggc
     1801 tgctccgaca aagccatatc ggcaccctgt tgcccgtcca ccgccaggaa aacctcgtag
     1861 ccgctgcgga tcagccggcg cgagagcatg tcgcggttca tgtcattatc ttcaaccaac
     1921 agaatccttg gcatgcttct cccagcgtgt tcgttcttca gtaagcgagg cgctccgctt
     1981 cacgggtggg ttgttgcgcc aggcgtgcgc gcacggcgac ggccagttcg ctcagatacg
     2041 cttggttctt ggcgatgatc cgttcggtct gtccgctcaa aatgcgataa tcctccgcgg
     2101 taagatcctt cgccgtcacg acgataactg ggatatccac gaatgcaggt cgtttacgca
     2161 gttcctgcag gaactcgaag ccgtcgagtt ccggcatcag caggtcgagt aaaattaggc
     2221 ttggggctgg gtggttgcca agccagtcca atccgctccg gccattgacg gccgcatggg
     2281 ccacatatcc catgctcacc accgaccgac agagcatttc gcgggtcgcc aagtcatctt
     2341 cgacgaccaa tatcgacgcg ctactcggac tggcgcaatg ctctcgcagt atcgccgtca
     2401 ggcgctgccg atccacaggc ttcgccagaa aatcggcggc gcccaatggg atcgccatgc
     2461 cacgctcgtt gagaacggtc accatgatca cgggtatgtc acgcagctgg gcgtcggcct
     2521 tgagttcctt gagcgctgcc cagccatcca tttgcggcat caaaacgtcg agcgtgatcg
     2581 cctgtggtct gtgctcgcgt gcgagcgcta gcgcctcgat cccgttgcgg gccgatatga
     2641 cacgatagcc ttccttcccg agggtcttgg caagcaggtt gcacaccatg ggatcatcgt
     2701 ccaccacaag cacagtcgca cgtccgtctg ccgtttccac cgccggtgca ggcgactccg
     2761 cggcagcggg gacaacgccg taatccggaa gcgtgatggt gaatgtcgag cctgtgccgg
     2821 gcatgctctc gaccgtgacg ttgccgccca gcatggtgca gaagtgcttg gtgatggata
     2881 gcccaagacc cgtgccgccg aagcgcctgg tggtcgaggc gtacgtctgg ctgaatgcct
     2941 cgaacagctg gccgagctgt tcctcagtca tgccgatccc cgtgtccgac acgcggaaac
     3001 tggctcgcga attgtcctcg cgcgccacca taagcgtcag cgtgcccttg ctcgtgaact
     3061 tgttggcatt gctcaacagg ttaagcaaac attgctttac cttggtgtgg tcggagcgaa
     3121 agctgccaat atccgccggg cagatcacct cgatgctatt ctcattcttg tcggccaacg
     3181 gcttgacgat cgacagcacc tccttgacca gagcttgaac gtctacctcc tcgatgaaga
     3241 tatccatctt cccggcctcg atcttcgaca ggtcgagaat gttgttgatc agtcccaaca
     3301 ggtgccggcc ggcgctttcg atcttctgca aatcgtcgat gggctctttg tcctccttgt
     3361 cggcggcgtc ctcttgcaga atttcgctgt agccgatgat cgcatttagc ggcgtgcgca
     3421 attcgtgcga catgttggcg aggaagctcg acttagtgcg gctcatcgct tcggcctgat
     3481 cgcgcgcggc cgtgagagca ttctcataat gcttgcggtc ggtgatgttt gtataaatgt
     3541 tgacgacccc cccgtgcggc agaggattat gccgaatttc cagaacggta ccgttaggcc
     3601 gcgtccgctc ggtcgaatac cgccccgcag acctcgccgt gaactgctga acctcggcgc
     3661 cgacgtcgac atcgccgtac tcgccccttt cggcctggaa acggatgaaa tcgctgaaat
     3721 gcggctcgcc agccaagaac gtctcaggta gttccagcag cttcgtaatt tgccgattcc
     3781 aagcaacgag tttcatctca cgatcgaata tgagcactcc gtgctccatg ttttcgaacg
     3841 tgacccgcat cacctcgccg cgccgctgca gttcggcggt gcgctgctca acgatctttt
     3901 ccagcttgat ggctgcatcg gcgcgttccg cgagcaattg atccagctcc acggcgtttc
     3961 tccggaatac ttcgacggct cttcccatgg cgccgatctc gtcggccccc gtcaccggaa
     4021 ccgcgctttc gcgtcggccc gccgcaatgg ccaacatcgc gctgctcaaa tgtgcgaggc
     4081 gggacacaat gttgcgcccg acgtacagcc aggcaatcaa gcctgcggcg acgagggcca
     4141 caatcgagag cgcgataaga aggattcgac ccgaacggat ttgctcggcg gcgcgcgcgg
     4201 aaaccgctgc ggcgtccttg ccttgttgct cgacaagacg gtcgaccgcc tgcttgagct
     4261 tcacggcctc gttttgcgtt gccgcgaatg caagctgtgc ctcctgctgc cgcaacaact
     4321 cccgcgcgcg cacatcaact atcccgtcct ggcccgccat ggcggcgagc cgctcgtgga
     4381 gatcgattag tcttttccgc tgttcgggat cggcaagcgc attgaggctc gtttcgatcc
     4441 tgcgccgcgc ggcgtcgatg ttttcgcgca gcggctgcag gcgcacgctc tcggtaacca
     4501 tcgaactttc gatcagcaag ccagcgagca aattggcctc ggcctgcact tcgagcagat
     4561 ggcgcagcga ctcgatcgcc
//
LOCUS       DATLJT010001304        12957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_104177,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001304 DATLJT010000000
VERSION     DATLJT010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104177"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VM910_29935"
     CDS             complement(<1..347)
                     /locus_tag="VM910_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HVI66762.1"
                     /translation="MHLLSDAKVSSPILAAGGIVLAEGSRPRIAIVRLRRDKSWVLPK
                     GKLYPGEHALAAARREVLEETGHEVSVHGFLGSMLYSVDGRIKIVQFWHMRAIGGPRR
                     ELMDDIKSVKWVS"
     gene            545..1750
                     /locus_tag="VM910_29940"
     CDS             545..1750
                     /locus_tag="VM910_29940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI66763.1"
                     /translation="MKLRLDTKTVPALALGKGQRELFAWDSELAGFGLRLQGQRRTYI
                     AQYRAGGHTRRVTLGTTERLTPTQAREGVRKLLARVSLGEDPQDEKAAQRTAAERTFR
                     KVVEAYLAAKRSTLRPASYNVTQLYLTGPYFRPLHTHGIGEIKHPDIAAQLSAITRKH
                     SANTAGAARRAISALFRWSMEEGWAKANPVVGTRKPADPKPRDRVLTDAELVAIWNAC
                     GDDDFGRIVRLLVLLGSRSREVGGMRANELDLEAGTWELPEERSKNHRAHLIDLPPAA
                     LKIIRMVFPLGERDHLFGVRAHHGYTAWGHGWTALAARLKGTVKPWQLRDIRRTVATR
                     MGDIKIHPHVIEAVLNHHSGYRAGVAGVYNRSPYRDEVRAALVRWSERVLALAEGRDE
                     RKAVPLHAC"
     gene            1776..2753
                     /locus_tag="VM910_29945"
     CDS             1776..2753
                     /locus_tag="VM910_29945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66764.1"
                     /translation="MAGIEGDLTIWVLLHDAVLLVERYTKSNLEAQWLILEYANKRHF
                     TECRFSGGQFIDPRFWNAHHPRFGLYIPVDFRNSTVTRLRAEPTEYLLPEMLVEKLKE
                     LPAPAARRERHARRSMPPEARLAARAEMLVEKFKELPTPEARLAARAAARAWPKPAWA
                     GLCPPRSLQMHLVCLAHHELVSMLYKAGLLELSDPPPAPPMPEPRGVPEPTSEKEERP
                     RPEPASKRTGKETPEWPSAEMLGLGPRAWQQRRIWTEMPEIYRRHPERFDSNRMWNRK
                     ASDLLISVQNLRDDKARAEGKASKAVSPDSCEDLLKAWKAWQTRHRASN"
     gene            3042..3269
                     /locus_tag="VM910_29950"
     CDS             3042..3269
                     /locus_tag="VM910_29950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66765.1"
                     /translation="MSTEALFAERPPSWIELESVKRMAVASQMTSLSGDALRRNYPDL
                     IVKLSPKRDGMKLKDILAIANGTARRADKPE"
     gene            3485..3892
                     /locus_tag="VM910_29955"
     CDS             3485..3892
                     /locus_tag="VM910_29955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66766.1"
                     /translation="MLDIVKSSPICNGNGAGEINGRGARRRKLTPEQRVSLAADIALR
                     LTPFAPSIGQTAAATGVSPYSIRKELKARSAAKELKARSAAAERERQAEYSRQGALPI
                     VSTWDRATVEGRTEAVRLIGPATVWDVLADVVG"
     gene            3955..4218
                     /locus_tag="VM910_29960"
     CDS             3955..4218
                     /locus_tag="VM910_29960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66767.1"
                     /translation="MKTVKNKHSGFDIDDNDWVVKHGSGRKQRTIYQTSDGNFFLRYA
                     DEVEVEAISELEARQLVSAWEFIDELVSAFRTGTPPNIKKYLG"
     gene            4282..4458
                     /locus_tag="VM910_29965"
     CDS             4282..4458
                     /locus_tag="VM910_29965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66768.1"
                     /translation="MTDMPTADRKAKLEFELAHIEHEIRNKQHDITCLQEELSDLEIE
                     LEELREQLEETTQA"
     gene            complement(4705..5247)
                     /locus_tag="VM910_29970"
     CDS             complement(4705..5247)
                     /locus_tag="VM910_29970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66769.1"
                     /translation="MLMAKDQPAKVLVPALRLFACCDDVPGRHGWTRSQQIDPCKRQL
                     GEPMRCIQTTLQKNRRYLALLRPDHRKRVTDEGFDVIEWRIENIVCPAWSLALEEIEA
                     AVKVLIASIYDIAGEHLVAGTVRGFGNVADAATRVPYRIGERFGLEQPLRAPFRLNVE
                     AVRLLAPHVCGQGTAILVLK"
     gene            5295..5771
                     /locus_tag="VM910_29975"
     CDS             5295..5771
                     /locus_tag="VM910_29975"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HVI66770.1"
                     /translation="MTDAVTITINGEVVAKGRTRMTRRGFVYTPTATRKYEAHARLAA
                     QVAMGDRAPLQIPVRVELAVELPVPTSWSNKRRLAALAGAIAPTSKPDIDNYVKMALD
                     AINTIVVTDDALVVEIQARKRFSEQPKLTAIIIPLDVAASNSRIPQREAVAVAQEV"
     gene            5778..6212
                     /locus_tag="VM910_29980"
     CDS             5778..6212
                     /locus_tag="VM910_29980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66771.1"
                     /translation="MNAFLEFADKQTPPAVKARQRAAEKRRVTKTEKKALAEHDYLFR
                     LWKKWRRERLEALLMGPHGAAAHKLVAFLQAMKLEDGTRLVEFVRAAGWAHTDADTKF
                     EVLSLINATITALRERAGLAPIDDPLPHEQPTAFLIIRELFR"
     gene            6209..8341
                     /locus_tag="VM910_29985"
     CDS             6209..8341
                     /locus_tag="VM910_29985"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI66772.1"
                     /translation="MKGKPKEAVPEARPYARTLFAQFTQPFIIPARDLEQGSTRLVFD
                     LEGNGLLETITCLHCVVIGELDNDHIYEYGPEQIADALAHLTLADTLIGHNIQSYDLP
                     VLRKLHEWTPRPATRLVDTLIAGRLILPHLPDIDSEVAARPKDKAFGQIRGKYSLEAW
                     GVRLGVAKIGAELEDWSRWSPEIQTRCVGDVIINKRLWQFLQPDGYPRAALELEHGVA
                     AICDRIAADGVPFDIQAAERLSESWKVRRAELAKRLHEQFPALKNPNSRQQIAAVLKE
                     RGWQPKKLTEKTKQPVIDEELIDSLPDTYPEFTGLSEYHLLGRRLGSLANGKEAWIKH
                     VHDGCIYSNLVHIGTPHSRAKHFGPNVAAVPNPKKGSAFGVECRALFHHPGDWVFVCC
                     DQATLQDRGFAHYLAAYDNGAYARTLVNGTIDQHWHTATALGLVLEARDKSNKEHTVI
                     REGAKTFRYAFLFGAGDLCAGQNIADIVHATTVVTPDSPLCEKILAGDKHPSEAVLRR
                     IGRRARDKFIAATPGLQALRQRLSAEHRRRGWLEGLDGRRIPTGADYKGLNRIVTAAE
                     AVICKRWLIDAYTELCARFRYGPGGDAYLTLWVHDELVVCCRPTIAEQVGKLLVRHAC
                     KAGEPYGFRVPLAAEFKIGRDWAGTALEPVANLAPALSTTIQDAAEEIAASGREFRLT
                     HSPIATAAPSLIAAAPAAEEEVVYIDDF"
     gene            8343..9407
                     /locus_tag="VM910_29990"
     CDS             8343..9407
                     /locus_tag="VM910_29990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66773.1"
                     /translation="MTIFRAALTDYAQSLYGPRKDRAFTLGASDIGQCSRRVFFSKHG
                     GERDPDYVETWGATLRGTIIEKEFWVPALRARFGAKLKFIGDQQRQFKRGFISATPDA
                     LLTEAPHDILAPLGVPDISSDCLLLESKSVDPRVKLDAPKSEHRYQVIVQLGVVCETT
                     EFQPRFGLLTYIDASFWDNITEFVIEFDPNVYASAKTRATKVLTARSASELPPEGWIA
                     GGKECEHCPFTKACGIARRAIPNGNDCADPQFAAEMRELARAYKARQADVDAAETRLR
                     ESQHTIKERLRAKQLCRVVGNDFSVTWTPVKGRQGYDLKALSSAAAAAGVGVQEFETS
                     GTLGDRLDVRVRETVELVKK"
     gene            9481..10152
                     /locus_tag="VM910_29995"
     CDS             9481..10152
                     /locus_tag="VM910_29995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66774.1"
                     /translation="MSNEGDKNVPIVPGKNPYEDYADRVDNQMLLGQLLKFSKGDWLI
                     GRDGDACPHTELVALMQGLMHGWTRWEDSAPVEHIMGLLVEGFVSPARNKLSHTDKAL
                     WEPNADGGEPQDPWQQSVYLPMITIDGDAVFTFATSSDGGRRRAIAPLCREYGNQMRQ
                     HPNELPVVGLEQDSYQHPNRKIGRVKYPLLPFKRLVKAEPYFAAVMTLTGKSLKLALP
                     SGKAA"
     gene            complement(10177..10557)
                     /locus_tag="VM910_30000"
     CDS             complement(10177..10557)
                     /locus_tag="VM910_30000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66775.1"
                     /translation="MLDFFRRFLDLAEVNIGVRADDQDHRLFSAASGLILDRIDADEA
                     AFSRPIMAASEIGDLGRATGEDVLFFGFARILDVWQAGDIDATVLVRSEQRDDEFGGG
                     VRGTHDNPFRYAREGREPDPPAAK"
     gene            10744..12723
                     /locus_tag="VM910_30005"
     CDS             10744..12723
                     /locus_tag="VM910_30005"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HVI66776.1"
                     /translation="MRLPGTINSKNGEQHEVRVLSDHTELRYELADLEQWAAATQEPL
                     LHRKNATGETGNGASPDNPFLAFAAAYIDEAPLDVDQLLADMVYLGAGGGGNAHDTLL
                     RCSAALLTRGEKREVVIERCLAALALAAVRSGLTIDPTREQSIIADMCDSWIAKHPPA
                     DNSSTKTNDKPSANIPAEPATSPVDLWNAFEPPRLPHGLLPSVIEDFAFEQSIAMGAD
                     PAGLAASALTVCATAIPDRIQLRVKRHGGWIEATRLWVALVGGSAAKKTPIIRQAVRP
                     LERIDAALWHAYATAKAAWDALDKETKRATPPPPQIRIKLDDVTVEAAQEVLRDSPDG
                     VLYHRDELSGFFGAIDKYAGNRAAANDRGFWLQAYNGDPYTFDRVKRGSGRIEHLSVS
                     VLGGIQPEPMRKLAADTVDDGLIQRLNPIMLQPGSIDRDEELSGAVESYEALVEELHQ
                     MQPPTLDDVTFEDAARAIREEIARKHCELVTIEIVNKKLSAHIGKYDGIFARLCLLWH
                     CIENPAATALPQISATCARRVADFLSRFLLPHAIAFYTSIYGLSDDHDRLTAVAGYIL
                     AHKVDRLTNRVIQRGDSTMRGLKRHEIENVCHQLNALGWITEAPRRRPTDLPRWDVNP
                     EVHRLFQERAKLEAAQRQRMREAIAEHVKSKEEVS"
BASE COUNT         2788 a         4067 c         3809 g         2293 t
ORIGIN      
        1 aacgacaccc atttcacgga cttgatatcg tccatcagct cgcgccgcgg tccgccgatg
       61 gctcgcatat gccaaaactg tacgatcttg attcggccat ccaccgaata caacatcgaa
      121 ccaagaaatc cgtggactga aacctcgtgc ccggtttctt ccaacacctc tcgtctggcg
      181 gcagcaagcg cgtgctcacc cggatagagc ttgccctttg gcaggaccca agacttgtcc
      241 cttcgcaatc gcacgattgc gatgcgcggt ctgctgcctt ccgctaagac aattccgccc
      301 gcagccagaa tcggcgatga aaccttcgcg tcggatagaa gatgcatggc ggttccccgt
      361 gatcgtgagc gccgcgcatg ctggctcggc atacttaacg cgaggttaaa atcggcatcg
      421 ccgcaggaga ccgcaggtgc cgcaggcgct tgaatttcag gcacttccgc gttaaccatt
      481 ggcgcaggtg gcgcagaagc cgcttcctac tgcttcctat gtgcttccta agtttgcgtt
      541 aaccatgaag cttcgactcg acactaagac cgttcccgct ctcgccctgg gcaaaggtca
      601 gcgtgagctc tttgcgtggg acagcgagct cgccggcttc ggcctgcgcc tccaagggca
      661 gcggcgtacc tacatcgcgc agtatcgcgc cggcgggcat acccggcgcg tcaccctcgg
      721 caccaccgag cggctcaccc ccacgcaggc gcgggagggc gtacgcaagc tccttgcccg
      781 ggtctcgctc ggggaggatc cgcaagacga gaaggcggcg cagcggaccg cggccgagcg
      841 caccttccgc aaggtggtcg aggcttatct cgcggctaag cggagcacgc tgcggccggc
      901 ctcctacaat gtgactcagc tttatctcac cggcccctat ttccgccccc tgcacaccca
      961 cgggattggc gagatcaagc atcccgatat cgccgcccaa ctctccgcaa ttacccgcaa
     1021 gcacagcgcc aacaccgcgg gcgccgccag gcgggccatc tccgctctgt tccgctggtc
     1081 catggaagag ggctgggcga aggccaatcc agtggtcggc acccgcaagc ccgccgatcc
     1141 caagccgcgc gatcgtgtgc tcaccgacgc cgagcttgtc gcaatctgga acgcctgtgg
     1201 cgacgatgac ttcgggcgta tcgtccggtt gctcgtattg cttgggagcc ggtcgcggga
     1261 agtgggcggc atgcgcgcga acgagctcga tctcgaggct gggacctggg agctgcccga
     1321 ggagcgctcc aagaatcatc gcgctcacct catcgatctg ccgccggcgg cgctgaagat
     1381 cattcgcatg gtgtttccat tgggcgagcg cgatcacttg ttcggagtgc gcgcgcatca
     1441 cggatacacg gcatggggac acggctggac ggcgctcgct gctcgcctca aggggaccgt
     1501 gaaaccctgg cagctacgcg atatccgccg gacggtcgcc acccgcatgg gcgacatcaa
     1561 aatccatcca catgtgattg aggcggtgct taatcatcac agcgggtacc gggctggcgt
     1621 cgccggcgtt tacaaccgtt cgccgtaccg ggatgaggtg cgggccgcgt tagttcgctg
     1681 gagtgaacgc gtgctcgctt tggccgaagg acgcgacgag cgtaaggctg tgccgcttca
     1741 cgcctgctga agtgggccgg ccagcggaag actccatggc cgggattgaa ggtgatctta
     1801 ccatatgggt gctgctgcac gacgcggtgc tcctcgtcga acgctatacc aagtcgaatc
     1861 tcgaagccca gtggttgatc ctcgaatacg ctaacaagag gcacttcacg gagtgtcgct
     1921 ttagcggcgg gcaattcatt gatccgcggt tctggaacgc ccaccatccg cgctttggcc
     1981 tctacattcc ggtcgacttc cgcaacagta cggtgacgcg cctgcgcgca gagccgactg
     2041 aatatctgct ccccgaaatg ctagttgaaa aattaaagga gctgccagcc ccagccgccc
     2101 ggcgcgaacg ccacgcccga cgctcgatgc cccccgaggc tcggctcgct gcgcgagccg
     2161 aaatgctagt tgaaaaattt aaggagctgc cgacccccga ggctcggctc gctgcgcgag
     2221 ctgctgcgcg cgcctggccg aagccggcct gggccggcct atgccctccc agatccttgc
     2281 agatgcatct tgtgtgcctt gcgcaccacg agcttgtttc gatgttgtac aaggccgggt
     2341 tgttggagct gtccgaccca ccgccagcac caccaatgcc cgagccgcgc ggggtccctg
     2401 aaccaacatc agagaaggag gaaaggccac gccccgagcc agcttcgaaa cggacaggga
     2461 aagagactcc ggaatggcca tccgccgaga tgcttggact ggggccacgg gcttggcagc
     2521 agagaaggat ttggacggag atgcccgaga tttaccgcag gcatccggaa cgctttgatt
     2581 cgaataggat gtggaatcgt aaggcgtcag acttactcat ctccgtgcag aaccttcggg
     2641 atgacaaggc gagggcggaa ggtaaggcat caaaagccgt aagcccagac tcgtgtgaag
     2701 atcttctgaa ggcatggaag gcatggcaga caagacaccg tgcttccaat tgatcggcgc
     2761 tcttgcgagc gcaaacctcg tcctttagct ggcagcagga gcccaggtcg cggtcggaaa
     2821 ccccggcctg aaggccgggg agtagtcata tcttttctcg tcgcgtttgg cgccaggcgg
     2881 agcttttgcc tcgtagcgga tttcttaccg cgtagcggaa atccgctagc ggaaaagttc
     2941 ttccgctagg cggataaatc taacgtcgtc tcgccccatg tttgttctca agtctgccga
     3001 tcgcgcctat gttgccgcgc aggcgcagga ggtcgccccg agtgagcacc gaagcacttt
     3061 tcgccgagcg tcccccgagc tggattgagc tcgagagtgt caaacggatg gcagtcgcgt
     3121 cgcagatgac gtcgctctcc ggtgacgccc ttcggcgaaa ttacccggac ctcattgtca
     3181 agctgtcccc caagcgcgat gggatgaaat tgaaagacat cctcgccatc gccaacggga
     3241 ccgcgcggcg ggccgacaaa cctgaatagc tgctgagttt gtacgtgcgt accaacgcgg
     3301 gcgcggcaat ggctgtgtct acgaacagga aaggtgtgtc cgatgcaaca gcggatcgtc
     3361 tgcgatgcca ccggcagacg agacggaact agcacagtgt ggcgtgaggg gccggcgatg
     3421 tccccaacgg ttcgaagtcc aggacatgcc ggccgccaag tgagcgccaa ggaggcgctt
     3481 ttgcatgctc gatatagtca aaagttcccc aatctgcaac ggaaacggtg cgggcgagat
     3541 caacggccgg ggcgcccgtc gccggaagct cacccccgaa cagcgcgtca gcctcgcggc
     3601 cgacatcgcc ttgaggctca ccccgtttgc accctcgatc ggtcagacag ctgcggcgac
     3661 cggcgtcagt ccgtacagca ttcgcaagga attgaaagcg cgctccgccg cgaaggaact
     3721 gaaagcgcgc tccgccgcgg cggagcgaga gcgtcaggcc gaatattcgc gccaaggtgc
     3781 gttgccgatc gtttcgactt gggatcgcgc cacggttgag gggcgaacag aggcggtccg
     3841 gctcatcggc cctgccactg tttgggacgt gctcgccgac gtagttggct gacaattcac
     3901 tgggcatcgg cgcgcaaatg cggcggcgct tttttcgcgg aaggaaaacg acctatgaaa
     3961 acggtaaaga acaagcactc cggctttgac attgacgaca acgattgggt tgtcaagcac
     4021 gggtccgggc ggaagcagcg caccatctac caaacctcgg acggaaattt tttcctgcgc
     4081 tacgccgacg aagttgaggt tgaggcgatc tctgaattgg aggcacgcca gctagtaagt
     4141 gcttgggagt tcatcgacga actcgtaagt gccttccgta ctggaacgcc tccgaacatc
     4201 aaaaaatatc tgggctgaaa ccagggcagc gctgacgcac gaatgcggcg gcaccctctc
     4261 tcacatcgaa agagaattcc gatgactgac atgcccaccg ctgatcgtaa ggccaaactc
     4321 gagttcgagc tggctcatat tgagcacgaa attcgcaaca agcagcacga catcacctgc
     4381 ttgcaggaag agctctccga ccttgagatc gaactcgagg aattgcgcga acagcttgag
     4441 gagaccaccc aagcatgagt ttccaggagc gcaggtcgat ctccatcggt cagcggcgag
     4501 atcgagaaac gaaagacagc tctgcagtgt gccctggaaa aaagagaatg cgttctcgcc
     4561 caaggctacc gatcgccgca ctttctcaat ggagtcaagt aatgaaaccc atgatggcca
     4621 gattaaattt acccgcaatg cgagccgtgc tgaatgagag ggcagcgtga agtcatgaca
     4681 atcgaccccg gtactcaact cgacctattt aaggacgaga attgccgtcc cttggccgca
     4741 cacgtgtggc gcaaggagtc gaacggcttc tacgttgagc cggaatggtg ctctaagcgg
     4801 ctgttccagg ccgaaacgtt caccaattcg atatgggacc cgtgttgcgg cgtcggccac
     4861 attgccgaat ccgcgcaccg tgccggctac gaggtgttcg ccagcgatat cgtagatcga
     4921 ggctatcagc actttaacgg ctgcctcgat ttcctccagt gcgagcgacc acgcggggca
     4981 aactatgttt tcaatccgcc attcgatcac gtcgaagcct tcgtccgtca cgcgctttcg
     5041 atgatccgga cgaagaaggg caagatagcg acgatttttc tgcaacgtcg tttgaatgca
     5101 gcgcattggc tcgccgagct gccgcttgca cgggtctatc tgttgactcc gcgtccatcc
     5161 atgccgcccg ggcacgtcat cgcagcaggc gaaaagccga agggcgggaa ccaagacttt
     5221 tgctggctgg tcttttgcca tcagcaaaag ggcccgccgg aattgcgatg gctacatcgc
     5281 gacgggattt gtcgatgacc gacgcggtca ccatcaccat caacggcgaa gtggtcgcga
     5341 agggccgaac gcggatgacg cggcgtggct tcgtctatac gcctaccgct acgcggaaat
     5401 acgaagccca cgcccggctc gccgcgcaag tcgctatggg tgatcgggca cccctgcaaa
     5461 tccctgttcg agtcgagtta gcagttgaac tgccagtgcc caccagctgg tccaacaaac
     5521 ggcgacttgc cgcgcttgcc ggtgcaatcg cgcccacctc caagcccgat atcgacaact
     5581 atgtgaagat ggcgctcgac gcgattaaca ccatcgtcgt caccgatgac gccctggtcg
     5641 ttgagataca ggcccgtaag cgcttcagcg aacagcccaa gctgacggca atcatcatcc
     5701 cgctcgacgt agctgcttcg aattcgcgaa tcccacagcg cgaagccgtc gccgtcgcac
     5761 aagaggtttg aacggccatg aacgcttttc tcgaattcgc cgacaaacag acaccaccag
     5821 cagtaaaagc gcgccagcgc gcggccgaaa agcggcgcgt aacgaagacc gaaaaaaagg
     5881 cgctcgccga acacgattac ctgttccgct tatggaagaa atggcggcgc gaacggctcg
     5941 aggcattgct gatggggccg catggcgcgg ccgctcacaa gctagttgca ttcctccaag
     6001 caatgaagct cgaagatggg acacgcctgg tcgaattcgt tcgcgccgcg ggctgggcac
     6061 ataccgatgc tgatacgaag ttcgaagtcc tgtcgttgat caacgccacg atcactgcgc
     6121 tgcgcgaacg cgccgggtta gcgccgatcg atgatcccct accgcatgaa cagccaaccg
     6181 cattcttgat cattcgggag ctgttccgat gaaaggcaag cccaaagaag ctgtgccgga
     6241 agcgcggccc tacgcccgca cgctgttcgc gcaattcacg caacccttca tcatccccgc
     6301 gcgcgatctc gagcaaggat cgacacgttt ggtttttgac ctcgagggaa atggcctact
     6361 cgagaccatt acctgtctgc actgcgtcgt catcggcgag ctcgacaacg atcacatata
     6421 cgaatatggc cccgagcaaa ttgcggatgc gctcgcacat ctgacgctcg ccgatacgct
     6481 aatcggccac aacattcaaa gttacgacct gccggtattg cgcaagttgc acgaatggac
     6541 gccgcgaccg gcgacccggc tcgttgatac gctgatcgcc gggcgattga tccttccgca
     6601 tcttcccgac atcgacagcg aggttgcggc acggccaaaa gacaaagcct ttggccaaat
     6661 acgtggaaag tatagcctcg aggcctgggg tgtgcgactc ggcgtggcga aaatcggcgc
     6721 tgagctcgag gactggtcta gatggtcacc tgaaatccaa acccgctgtg tgggtgatgt
     6781 gatcatcaac aaacggctgt ggcaattttt gcagccggat ggctaccccc gcgcggcact
     6841 cgaacttgag cacggcgtcg ccgctatctg cgaccgcatc gccgccgatg gcgtaccctt
     6901 cgacatacag gcggcggagc gcttgagtga gtcctggaaa gtacgacggg ccgaactcgc
     6961 aaagcgattg catgagcaat tcccagcact gaagaacccc aactcacgac agcagatcgc
     7021 tgcggtactc aaggagcgcg gctggcagcc gaaaaaactg accgagaaaa ccaagcagcc
     7081 ggtcattgac gaggaactga tcgactccct ccccgatacg tatccggaat tcaccggact
     7141 gtcggaatat cacttgctcg gtcggcggct cggatcgctg gcaaacggca aagaagcgtg
     7201 gatcaaacac gtccatgacg gttgtatcta tagcaaccta gtgcacatcg gcacgccgca
     7261 cagccgcgcc aagcactttg ggcccaatgt cgcagcagtg ccaaatccca agaaaggttc
     7321 ggcctttggc gtcgagtgtc gcgcgttatt ccaccacccc ggcgactggg tctttgtttg
     7381 ttgcgatcag gcgacgcttc aagaccgcgg ctttgcacac tacctcgccg cctacgacaa
     7441 cggcgcctat gcgcggactc tcgtcaacgg gacgatagac cagcattggc acaccgcgac
     7501 cgcgctcggc ctggtcctgg aggcgcgcga caaaagcaac aaagaacaca ccgtcatccg
     7561 tgagggggca aagaccttcc gctatgcctt cctctttggt gccggtgact tatgcgcggg
     7621 gcaaaacatc gctgacattg tccatgcgac gacagtcgtc acgccagaca gcccgctctg
     7681 cgaaaaaatc ctggccgggg ataagcatcc aagcgaggct gtcttgcggc gaattggtcg
     7741 gcgggcgcgc gataaattca ttgccgcaac acccggccta caggcactgc gccaaaggct
     7801 ttccgccgag caccggcggc gcggctggct cgaagggctc gacggtcggc ggattccgac
     7861 tggagccgat tacaagggcc tcaatcgcat cgtcaccgcc gccgaagccg tcatctgtaa
     7921 acgctggctc atcgacgcgt ataccgagct ctgcgcgcgc ttccgttacg gcccaggcgg
     7981 tgacgcctac ctgacactgt gggtgcacga cgagctcgta gtctgctgcc gcccaaccat
     8041 cgccgaacag gtcggcaagc ttttggtgcg gcacgcgtgc aaggccggcg agccctacgg
     8101 tttccgcgtg ccgctcgctg ccgaattcaa gattgggcgc gactgggccg gcaccgcatt
     8161 agagcccgta gcgaacttgg caccggcgct atccaccacg atccaagacg cggccgagga
     8221 gatcgcggcg tccgggcgcg agttccgcct aacgcattcg ccgatcgcca ccgcggcgcc
     8281 ctcattgatc gccgctgctc ccgccgccga agaggaggtc gtctatatcg acgatttctg
     8341 acatgacgat ttttagggcg gccctcaccg actacgcgca gagcctctac ggcccccgca
     8401 aagaccgtgc gttcacgctc ggcgcgagcg atattggtca gtgcagcagg cgagtgtttt
     8461 tctctaagca cggcggcgag cgcgacccgg attacgtcga gacctggggc gcaacgctac
     8521 gcggcacaat catcgaaaaa gaattttggg tgccggcgct gcgcgcccgt ttcggcgcga
     8581 agttgaagtt catcggcgac caacaacgac agttcaagcg cggttttatt tcggccacac
     8641 ctgacgcgct cctcactgag gcaccgcacg atatcctggc accgctcggt gttccagata
     8701 tcagcagcga ctgtctgctg ctcgaatcca aatcagtcga tccgcgggtc aagctcgacg
     8761 cgcccaagtc cgagcatcga tatcaagtaa ttgttcagct tggcgtcgtg tgcgagacga
     8821 ctgaattcca gccacgcttc ggccttctca cctacatcga tgccagcttc tgggacaaca
     8881 tcaccgaatt tgtgatcgag ttcgatccga acgtttacgc cagcgccaag acgcgcgcca
     8941 ccaaggtgct caccgctcgt tctgcatccg aattgccgcc ggaaggctgg atcgctggtg
     9001 gcaaagaatg cgagcactgc cccttcacca aggcgtgcgg cattgcgcga cgcgccatac
     9061 cgaacggcaa cgattgcgca gatccgcagt tcgccgccga gatgcgcgag ctcgcccgcg
     9121 cctacaaggc ccgccaggcc gacgttgacg ctgccgaaac gcgactacgc gaaagtcagc
     9181 acacaatcaa ggaacggctg cgcgccaagc agctgtgccg agttgtcggc aacgactttt
     9241 ccgtgacctg gaccccagtc aagggcaggc agggctacga cctcaaagcg ctgagctcgg
     9301 cggcggctgc tgccggcgtc ggcgtgcaag aattcgagac gtccggcact ctcggcgatc
     9361 gactggatgt gcgggtacgc gaaaccgtgg agttagtgaa gaaataaaac gaattcgagg
     9421 actggcagtg ttctcgatgt tcgcgtgcgc gaagcacgct cttagcaaaa ggagactgtt
     9481 atgagtaacg aaggcgacaa aaacgtaccg atcgtaccgg ggaagaaccc gtacgaggac
     9541 tacgccgacc gtgtggacaa tcagatgctg cttggtcagc tcttgaaatt cagcaaaggc
     9601 gattggctga ttggtcgcga cggtgatgca tgcccccaca cagagctggt cgcgctcatg
     9661 caagggctga tgcacggctg gacacgctgg gaagacagcg ctccggtgga gcacatcatg
     9721 ggacttctag tggaaggctt tgtctcacct gcacgcaaca agctcagcca caccgacaaa
     9781 gcgctgtggg agcccaacgc cgatggcggc gagccacaag acccctggca acagagtgtc
     9841 tacctaccga tgatcacgat cgacggtgac gccgtcttca cgtttgcgac ctcgagcgac
     9901 ggtggccgcc gccgtgcgat cgctccgctc tgtcgtgaat acggcaatca gatgcgccag
     9961 catccgaatg agctcccggt cgtcggactc gagcaggact cttaccagca ccccaatcgc
    10021 aaaattgggc gggtgaaata cccgctgttg ccgttcaagc ggttggtcaa ggccgagcct
    10081 tacttcgccg cggtcatgac actcaccggc aagtcactga agctagcgtt gccgtctggg
    10141 aaagcagcgt gatttctaca ctagggcaaa cccgatctac tttgctgccg gtggatcggg
    10201 ttcacgccct tcgcgcgcgt aacgaaacgg attgtcatga gtaccgcgca ccccgccgcc
    10261 gaattcatcg tcgcgctgtt cggaccgcac caaaacggtc gcgtctatat cgccagcttg
    10321 ccaaacatca agaatgcggg cgaagccgaa gaaaagcaca tcctcacccg tagctcggcc
    10381 cagatcgccg atttcactcg ccgccatgat cggccgggag aaggctgctt cgtctgcgtc
    10441 aatccgatca aggataaggc cactcgccgc gctgaagaga cggtggtcct gatcatctgc
    10501 gcgcacgccg atattgactt cagcaaggtc gaggaaacgc cggaagaagt cgagcacatc
    10561 gtcactggcc tatcgctgcc acccagccgg gtgcaccact ccgggcatgg gttacacatt
    10621 tactggttct tgaagatcgc gatcgcggcg acgccggaga acaatgaccg acataagcac
    10681 ctgctgcacc ggctcgccga cattctcggc ggtgacccgg gcgcttgcct gatcccacag
    10741 ctgatgcggc tgcccggcac catcaattcc aagaacggcg agcagcatga ggtgcgcgtg
    10801 ctcagcgacc ataccgagct gcgctacgag ctcgccgacc tcgagcaatg ggccgccgcc
    10861 acccaggagc cgctactcca ccgcaaaaac gcgaccggcg agaccggcaa tggcgccagc
    10921 cctgacaatc cattcctggc cttcgccgcg gcgtatattg acgaggcccc gctcgacgtc
    10981 gaccagctcc tggccgacat ggtctatctc ggcgccggcg gcggcggtaa cgcccacgat
    11041 accttgctgc gctgtagcgc cgcgctgctg acccgcggcg agaaacgcga ggttgtcatc
    11101 gagcgttgcc tcgctgccct cgccctcgcc gccgtacgtt ccggactgac gatcgatccc
    11161 acgcgcgagc aatccatcat cgccgacatg tgcgacagct ggatcgccaa gcacccacct
    11221 gcggataatt catcaacaaa gacgaacgat aagcccagtg cgaacatacc tgccgaaccg
    11281 gcaacaagcc cggtggactt gtggaacgca ttcgaaccgc cacgcttgcc gcacggatta
    11341 ctaccgtcag tgatcgagga cttcgccttc gaacagagca tcgccatggg cgcagacccc
    11401 gccggcctcg ccgcctccgc gttgacggtg tgcgccacgg ccattcccga tcgcatacaa
    11461 ctgcgcgtca agcgacacgg cggatggatt gaagcaaccc ggctgtgggt cgctctcgtc
    11521 ggcggttcgg ctgccaaaaa gacccccatc atccgccagg ccgtgcgccc gctcgagcgc
    11581 atcgacgccg cgctatggca cgcttacgcc accgccaagg cagcgtggga cgcgctggac
    11641 aaggaaacga agcgcgcgac accgccgccg ccacagatcc gaatcaagct cgacgacgtc
    11701 accgtcgaag cggcccagga ggttctgcgc gacagccccg acggcgtcct gtaccatcgc
    11761 gatgaactgt cgggcttttt cggggcgatc gacaaatacg ccggcaatcg cgcagcggcc
    11821 aacgaccgcg gcttttggct gcaagcctac aatggcgacc cctatacctt cgatcgcgtc
    11881 aagcgcgggt ccggccgcat cgagcacctg tcagtctcgg tgctcggcgg cattcaaccc
    11941 gagccgatgc gcaagctggc cgcagacaca gtggacgacg gactcatcca acgcctcaac
    12001 ccgatcatgc tgcaaccagg aagcatcgat cgcgacgagg aactgtcggg cgcggtagaa
    12061 agctacgaag ccttggtgga agaactgcac cagatgcagc cgcccacgct cgacgacgta
    12121 acgtttgaag acgccgcccg tgcgatccgc gaagaaatag cacgtaagca ctgcgaattg
    12181 gtcacgatcg aaatcgtcaa caagaagctc tcggcccaca tcgggaaata cgacggcatt
    12241 ttcgcccggc tctgcctgct gtggcactgt atcgaaaacc ccgccgccac ggcgctaccg
    12301 cagatcagcg cgacgtgcgc ccgccgagtg gcggacttcc tgtcacggtt cctgctgccg
    12361 cacgctatcg cgttctacac cagcatctac ggcctgtcgg acgatcacga ccggcttacg
    12421 gcggtcgccg gctacatcct ggcgcacaag gtggaccggc taacgaaccg cgtgatccag
    12481 cgcggcgaca gcacgatgcg cgggctgaag cgccatgaga tcgagaatgt ctgtcaccaa
    12541 ctcaacgcgc tcgggtggat cacggaggcg ccgcgccgac ggcccacaga tctgccgcgc
    12601 tgggacgtga acccggaggt ccatcgcctc ttccaagaac gggcgaagct cgaggcggca
    12661 cagcgccagc gcatgcgtga agccattgca gagcacgtaa aatcgaaaga ggaagtctca
    12721 tgagccaaaa cagcaatcac gttccagtag cactggacga ctggcgaggc tggatcacag
    12781 cacgcgtgca agagctcaag cgctattacc ctttacgtgt aggccgtcag cacttgtcgg
    12841 acagaatgag ctgattgcgt aagagaggat tgctggctca tcgtagcccc gctgaggagc
    12901 aacgatgaga caagcgagca agcgcatacc ggagagcgct gaaaagacag tccggga
//
LOCUS       DATLJT010001305         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1255169,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001305 DATLJT010000000
VERSION     DATLJT010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255169"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VM910_30010"
     CDS             <1..364
                     /locus_tag="VM910_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI66777.1"
                     /translation="VRILRGESMSAADYIDLVNARRSLIARAAARLAPYDALVMPTTA
                     NTPPRIADLADDKAFAVANLRSLRNCTLINMIDGCAISIPAHRDGEVPVGLMLASSGG
                     SDRRIFELAAGMEGAFRV"
     gene            357..1046
                     /locus_tag="VM910_30015"
     CDS             357..1046
                     /locus_tag="VM910_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HVI66778.1"
                     /translation="MFDLTFTVIDEDGGATPLTLPIDQAVIAGWTGRDPVARDKHIAE
                     LEAIGIARPATTPIYYRVSACRLTLEDVIEVCGKDSSGEVEFVLIGWQGRTFVGLGSD
                     HTDRKVESYSVTVSKQMCDKPMASTLWELEHVIDHWDKMILRSYAVTNGKRELYQVGT
                     LEAILPVGDLIRRGFEGGKLPDGCAMFGGTFAAKGGIRPADRFEYELEDPVLKRTIRH
                     AYDVIAMPVRG"
     gene            1194..1943
                     /locus_tag="VM910_30020"
     CDS             1194..1943
                     /locus_tag="VM910_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HVI66779.1"
                     /translation="MKTTARTAARLKPETEIVARVDRIDWNAAENELDAQGCAVLKGL
                     LSADECRALAALYPDDAHFRSRIVMGRHGFGRGEYKYFSYPLPGLIAELRPLLYARLY
                     GIANRWNAAMGIDIRYPQDHEAFLKRCHEAGQTRPTPLLLQYEAGDYNCLHQDLYGEH
                     VFPLQVAILLSEPGHDFEGGEFVLTEQRPRMQSRAEVVPLAQGEAVAFAVHHRPVQGT
                     RGIYRVNLRHGVSRLRSGRRHTVGVIFHDAK"
     gene            1940..2590
                     /gene="alkB"
                     /locus_tag="VM910_30025"
     CDS             1940..2590
                     /gene="alkB"
                     /locus_tag="VM910_30025"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HVI66780.1"
                     /translation="MNADLFESLDARPKREQLADGAMLLRGFALPVQHDLIAALDHIA
                     QQAPFRQMQTPGGHQMSVAMTNCGGFGWVTDRSGYRYAGVDPVSQKAWPPMPAVFLEL
                     AGQAAAQGGFPGFRPDACLINRYEAGARMSLHQDRDEKDFGAPIVSVSLGLPAIFLFG
                     GPKRSDRPERYRLEHGDVVVWGGVPRLFFHGVAPLTDGAHALLGRKRINLTFRKAS"
     gene            2662..3891
                     /locus_tag="VM910_30030"
     CDS             2662..3891
                     /locus_tag="VM910_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66781.1"
                     /translation="MDSTELTADAGTSGKRLGIYLAFLQLVFTLLWTTYVIYLPKLAA
                     SVGIAPTAVILILMMDQAIFTVTDTAMGIAADRIAALVGRLGIFVGLLTVLSCAAFVA
                     LPYVAALGAGAKMFLIALIVVWAITSSALRAPPLILLGKYSARPDIPFLSALVMLGYG
                     VAGAVSPYLGVVLRDVDARLPFVISSGVLLLTALGLSRVERGVALETAPAKRAEPAKP
                     FGTVPIIFIVAMIALALGYQLHFSINSAPFYLRFAKPDDLQWLMPVFWIGFNIAMFPA
                     SVIVKHRGGLLVMGAAGLFGAVAVIVAELAGDLSTLVIAQFVAGSAWGCMLMSAVSAA
                     LAIGETGAEGKVTGLVFSALALATFARMAAVAGGLQKLPDYAPLLHWAPVACWSVAGA
                     GLLVIAASRAQGSTQSV"
     gene            complement(3888..>4963)
                     /locus_tag="VM910_30035"
     CDS             complement(3888..>4963)
                     /locus_tag="VM910_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI66782.1"
                     /translation="APLPDADHVKDAAMLIAASKRPMIFVGSGAIDAREAVLELAETI
                     DAPVVAFRSGRGIVSNAHELGLTMAAAYRLWPETDMMIGIGTRLELPTMTRWPYQPDG
                     LKSVRIDIDPVEMRRYPSNVAIIADAKAGTRALAAAVGKAGYRKTAGRRSAIREASAA
                     ALKEIQKVQPQMAYLNILREVLPANAIVTDELSQVGFASWYGFPVYEPRTFITSGYQG
                     TLGSGFPTALGAKVANPNRPVVAITGDGGFMFGVQELSTAVQFNVGVVTLVFNNNSYG
                     NVRRDQRTRFDGRVVAADLVNPDFVKLAESFGVAASRVSSPDHFRPALEKALVHDGPY
                     LIAIEIPRDSEVTPWTFIHPTMP"
BASE COUNT          827 a         1549 c         1610 g          977 t
ORIGIN      
        1 ggtgcgcatc ctgcgcggcg agagcatgag cgcggccgac tatatcgatc tcgtgaacgc
       61 gcggcgttcg ctgattgcgc gggcggcggc acggcttgcg ccctatgatg cgctggtcat
      121 gccgacgacc gcgaatacgc cgccgcgcat cgccgatctc gccgacgaca aggcatttgc
      181 ggtcgcgaac ttgcgatccc tgcgcaattg tacgctgatc aatatgatcg acggctgcgc
      241 gatctcgatt cccgcacatc gcgatggcga ggtgccggtc ggtctgatgc tggcgtcgtc
      301 gggcggctcg gaccgccgca ttttcgagct cgcagccgga atggagggcg ccttccgtgt
      361 ttgatctcac cttcaccgtc atcgacgagg acggcggcgc gacgccgctg acgcttccaa
      421 tcgatcaggc cgtgatcgcc ggctggacgg gccgcgatcc ggtcgcgcgc gacaagcata
      481 tcgccgagct cgaggcgatc ggcatcgcgc gtcccgccac cacgccgatc tactatcgcg
      541 tctccgcgtg ccggctcaca ctggaagatg tgatcgaggt ctgcggcaaa gactcgagcg
      601 gcgaggtgga attcgtgctg atcggctggc agggccgaac cttcgtcggt cttggctccg
      661 accacaccga tcgcaaggtc gagagctatt ccgttaccgt ttccaagcag atgtgcgaca
      721 aaccgatggc gtccacgctc tgggagctcg aacacgtcat cgaccattgg gacaagatga
      781 tcctgcgctc gtacgccgtg accaacggca agcgcgagct gtaccaggtg gggacgctgg
      841 aggcgatcct gccggtcggc gatttaatca ggcgcggttt tgagggcggt aagctgccgg
      901 acggctgcgc aatgttcggc ggaacttttg ccgccaaggg cggcatccgc ccggccgacc
      961 gcttcgaata tgaactcgag gatcccgtgc tgaagcgcac cattcgtcac gcctatgacg
     1021 tgatcgccat gccggtgcga gggtaagtcg tcattgcgag gagcgtgagc tccgaagcaa
     1081 tccgatccga cctccgcatc gacatttcca gattgcttcg cttcgctcgc aatgacggat
     1141 cattcacgga aatcgggtgg tattcggcag tccggtttgc gagacttcga cctatgaaaa
     1201 ccaccgcaag gacagccgcc cgtttgaagc cggagaccga gatcgtcgcc cgcgtcgatc
     1261 ggatcgactg gaacgcggcg gaaaacgagc tcgacgcgca aggctgcgca gtcctcaagg
     1321 gattgctctc ggccgacgaa tgtcgcgcac ttgctgcgct ctatccggat gatgcgcatt
     1381 tccgcagccg catagtgatg ggccgccacg gttttggccg cggcgagtac aaatatttct
     1441 cctatccgtt gcccggcttg atcgccgaac tgaggccgct gctttatgcg cggctttacg
     1501 gtatcgccaa tcgctggaac gctgcgatgg gcatcgatat ccgctatccg caagaccacg
     1561 aggcgttcct gaaacgctgc catgaggcag gccagacccg tcccacgccg ctgctgctgc
     1621 aatatgaagc cggcgactac aattgcctgc accaggacct ctatggcgag catgtttttc
     1681 cgctgcaggt agcgatcctg ctgtcagagc ccgggcatga tttcgaaggc ggcgaattcg
     1741 tgttgaccga gcagcggccg cgaatgcagt cacgcgccga agtggtgccg ctggcgcagg
     1801 gcgaggcggt ggccttcgcc gtgcatcacc gtccggtgca gggcacacgc ggcatctatc
     1861 gggtcaacct gcgccatggc gtgagccggt tgcgctcggg tcgacgccac accgttgggg
     1921 tgatctttca cgacgccaaa tgaacgcgga tctttttgag agcctcgacg ctcgcccgaa
     1981 gcgggagcaa cttgccgacg gggcgatgtt gctgcgcggt tttgcgctgc cggtccagca
     2041 cgacctgatc gctgcgcttg atcatatcgc gcaacaggcg ccgttccgtc agatgcaaac
     2101 accgggcggc caccagatgt cggtcgcgat gaccaattgc ggcggcttcg gctgggtgac
     2161 cgaccgcagc ggttatcgtt atgccggcgt cgatccggtc tcgcagaaag cctggccgcc
     2221 gatgccggcg gtcttcctcg aattggccgg acaagcggcc gcgcaggggg gctttcctgg
     2281 ctttcgaccg gacgcctgcc tgatcaaccg ctatgaagcg ggcgcccgca tgtcgttgca
     2341 ccaggatcgc gacgaaaagg attttggcgc accgattgtc tcggtctcgc tcggacttcc
     2401 tgcgatcttt ctgttcggcg gtcccaagcg cagcgacagg ccggaacgct atcggctgga
     2461 gcatggcgat gtggtggtat ggggcggggt gccgcggttg ttctttcatg gcgtggcgcc
     2521 gcttaccgat ggcgcgcatg ccttgctggg ccgaaagcgc atcaatctga ccttccggaa
     2581 agcaagttag accttgctgc ggggccggtg ggaaaaacgt cacatttcct cgaccgtgcg
     2641 gtctggttta ttctcggtct catggactcg actgaactga ctgcagacgc aggcaccagc
     2701 ggcaagcgcc ttggcatcta tctcgctttt ctgcagctcg tattcacgct gctttggacc
     2761 acctacgtca tctatctgcc gaagctcgcg gcaagcgtcg ggattgcgcc gaccgcggtc
     2821 atcctgatcc tgatgatgga tcaggcgatt tttaccgtca ccgacaccgc gatgggcatc
     2881 gccgcggaca ggattgctgc cctggtcggc cggctcggaa tcttcgtggg cctgctcacc
     2941 gtgttgtcct gcgcggcctt tgtcgcattg ccctatgttg ccgccctcgg tgccggcgca
     3001 aagatgtttc tcatcgctct gatcgtcgtc tgggcgatta cctcctcggc gctgcgcgcg
     3061 ccgcccttga tcctgctcgg caagtacagc gccaggccgg acattccgtt cctgtcggca
     3121 ctggtgatgc tcggctatgg cgttgccggc gcggtgtcgc cctatctcgg cgtagtgcta
     3181 cgcgacgtgg atgcgcggct gccattcgtc atttccagcg gggtattgct gctgacggcg
     3241 ctgggtctgt cgagggtcga gcgcggcgtc gcgctggaaa ctgcgccggc gaagcgagcc
     3301 gagcccgcaa aacccttcgg caccgtgccg atcatcttca tcgtggcgat gatcgcgctc
     3361 gcgctcggct atcaactgca tttttccatc aacagcgcgc cgttttatct gcgcttcgcc
     3421 aagcccgacg acctgcaatg gctgatgccg gtgttctgga tcggcttcaa catcgcgatg
     3481 tttccagcga gcgtgatcgt caagcatcgc ggcggcttgc tggtgatggg cgcagcaggc
     3541 ctgttcggcg cggtcgcggt catcgtcgcg gaactcgccg gcgatctctc gacacttgtc
     3601 atcgcgcagt tcgttgcggg ctcggcctgg ggctgcatgc tgatgagcgc agtgtctgcg
     3661 gcacttgcaa tcggcgagac cggcgccgag ggcaaggtga cggggctggt attttccgcg
     3721 ctggcgctgg cgacctttgc gcgaatggcg gcggtcgccg gcggcttgca gaaactgccg
     3781 gactacgcgc cgctcttgca ttgggcgcct gtcgcgtgct ggtcggttgc gggcgccggg
     3841 cttctcgtga tcgccgcgtc gagggcgcag gggagcacgc agtcagtcta gggcatggtc
     3901 ggatgaatga aggtccacgg cgtcacttcc gaatcccggg gaatttcgat cgcgatgagg
     3961 taaggcccgt cgtggaccag tgctttctcg agcgcgggac ggaaatgatc gggtgagctg
     4021 acgcggctcg ccgccacgcc aaaagactcc gccagcttga cgaaatccgg attgacaaga
     4081 tccgcggcga cgacgcggcc gtcaaaccgc gtgcgctggt cgcgccgcac attgccgtaa
     4141 gaattgttgt tgaagacgag cgtcaccacg ccgacgttga actgcacggc ggttgaaagc
     4201 tcctgcacgc cgaacatgaa gccgccgtcg ccggtgatcg cgaccaccgg cctgttcgga
     4261 ttggcgacct tggctcccaa cgccgtcgga aagcccgatc ccagcgtgcc ctgatagccc
     4321 gacgtgatga aggtgcgcgg ctcgtagacc ggaaacccgt accaggaagc gaaaccgacc
     4381 tgcgacaact cgtcggtgac gatcgcgttg gccggcagca cctcgcgcag gatattgagg
     4441 taggccatct gcggctgcac cttctgaatc tctttcagcg ccgcagcgct tgcctcccgg
     4501 atcgcagagc ggcggcctgc ggtcttgcga taaccggcct tgccgacggc cgccgcaagc
     4561 gcgcgcgtgc cggccttggc gtcggcaatg attgcgacat tggacggata tcggcgcatc
     4621 tcgacgggat cgatgtcgat gcgaacggat tttagtccat cgggctggta gggccagcgg
     4681 gtcatcgtcg gcagctccag tcgcgtgccg atcccgatca tcatgtcggt ctcgggccag
     4741 agcctgtagg ccgccgccat ggtcagcccg agttcatgcg cgttggagac gatgccgcgg
     4801 ccgctgcgga acgcgacgac cggcgcatcg atcgtctcgg caagctcgag gactgcctcg
     4861 cgcgcgtcga tcgcacccga cccgacaaag atcatcggcc gtttgctcgc ggcaatcagc
     4921 atggccgcat ccttgacgtg gtcggcgtcc ggcagcggcg ccg
//
LOCUS       DATLJT010001306         1733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3552557,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001306 DATLJT010000000
VERSION     DATLJT010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552557"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VM910_30040"
     CDS             complement(<1..419)
                     /locus_tag="VM910_30040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195289.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HVI66783.1"
                     /translation="MDRIRIVGGKELKGTIPISGAKNAALPLMIAALLSEDTLILDNV
                     PRLADVAQLQRILGNHGVDITSLGKRPGDRPSQGQTLHISAANIIDTTAPYELVSRMR
                     ASFWVIAPLIARMREARVSLPGGCAIGTRPVDLLIMAL"
     gene            complement(599..1090)
                     /locus_tag="VM910_30045"
     CDS             complement(599..1090)
                     /locus_tag="VM910_30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66784.1"
                     /translation="MMLGLSLSAFTTLHVVISLIAILVGLIVMFGLLGSNRMPALTFI
                     FLLFTILTSVTGLMFPFEKLLPSHILSVLSLVLLAIACITLYGTGLSGASRWIYVITA
                     LASLYLNVFVLVTQGFLKIPALHALAPGNPPNGPAFAVVQGIVLLFFVIMTIGAVRRF
                     RPK"
     gene            complement(1226..>1733)
                     /locus_tag="VM910_30050"
     CDS             complement(1226..>1733)
                     /locus_tag="VM910_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI66785.1"
                     /translation="AVFLSGHARKVHMMIRGGGLGASMSRYLIERIEATPNIELMFNT
                     EVVGLESAHDASLERVRWRSRLSGEEETFDIRNVFLFVGADPATSWLDGCGVTLDRGG
                     FVVTGAQSDLTLGRPVSTLETSVPGVFAVGDVRSGSVKRVGGAIGEGAQVVAALHGFL
                     ADAAKPAL"
BASE COUNT          365 a          538 c          557 g          273 t
ORIGIN      
        1 agtgccatga tcagcaaatc gaccggtcgc gtgccgatcg cgcatccgcc gggcagcgaa
       61 acccgcgctt cccgcatccg cgcaatcagg ggtgcgatca cccagaagct cgcgcgcatg
      121 cgtgacacca gctcgtaagg cgccgtcgtg tcgatgatgt tggcggcgga gatgtgcagg
      181 gtctgcccct gcgagggacg gtcccccggg cgtttgccga gcgaggtgat gtcgacgccg
      241 tgattgccca ggatgcgctg caattgcgcg acgtcggcaa gccgcggcac gttgtcgagg
      301 atgagcgtgt cttcgctcag cagcgccgcg atcatcagcg gcagcgcggc attcttcgcg
      361 cccgatatcg gaatggttcc tttaagctcc ttgccgccga cgatacgaat gcgatccatg
      421 gcaattcctg cttgtatccc aagggaaatt cggggcgaaa ttcggggcga atgcgcaaag
      481 ccaaacagcc ctgatctttg cggcgatttg atggggtgtc gtgtcccggt gcgccgcgcc
      541 tgtgactaac gtgcgacggc tcgaggcaaa ccgggagatc cggccgctcg ctcggacttc
      601 acttggggcg gaatcgccgc accgcaccaa tggtcatgat gacgaagaac aacagcacga
      661 tgccctgaac gacggcgaaa gccgggccgt tcggtggatt gcccggcgcg agcgcatgca
      721 gcgcggggat cttgagaaag ccctgggtca ccagcacgaa tacattgaga taaagcgagg
      781 ccagcgccgt gatcacgtaa atccaccgcg acgcgccgga gagccctgtt ccatacagcg
      841 tgatgcaggc gatcgcgagc agcaccagcg agagaaccga gaggatatgc gacggcaaca
      901 gcttttcgaa cggaaacatc aggccggtga cgctggtcag gatggtgaag agcaaaaaga
      961 tgaaggtcaa agcaggcatc cggttcgagc cgagcaagcc gaacatcacg atcaggccga
     1021 ccaggatcgc aatcaggctg atgacgacgt gaagggtagt aaaggcggac aggctcagac
     1081 ccagcatcaa gacacgctcc cctcctcatg accggcgctc gatgctagtc ggcggtgaga
     1141 caaatttcca ctacagtgcc gcgtggcggc attgcgattc gatggtctgt tgcgaagctg
     1201 tcacaaatgc gagtaccggc gcgcgctaaa gcgcgggctt ggcggcatcg gccaaaaagc
     1261 catgcagcgc cgccaccacc tgcgcgccct cgccgatggc gccgccgaca cgcttgaccg
     1321 aacccgagcg cacgtcgcct actgcgaaca cgccgggcac cgaggtctca agcgtcgaaa
     1381 ccggcctgcc gagagtgaga tccgattgcg cgccggtcac gacgaaaccg ccgcgatcca
     1441 gcgtgacgcc gcagccgtcg agccaggagg ttgcgggatc tgcgccgacg aacaggaaga
     1501 cattacggat gtcgaaggtc tcctcctcgc cggagaggcg gcttctccag cgcacgcgct
     1561 ccagcgaggc gtcgtgcgcg ctctcgagcc caacgacctc ggtgttgaac atcaattcga
     1621 tgttcggcgt cgcctcgatg cgctcgatca ggtagcgcga catgctggcc ccgagcccgc
     1681 cgccgcggat catcatgtga accttgcggg cgtggccgga cagaaacacc gcc
//
LOCUS       DATLJT010001307         1576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3321955,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001307 DATLJT010000000
VERSION     DATLJT010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3321955"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..886
                     /locus_tag="VM910_30055"
     CDS             368..886
                     /locus_tag="VM910_30055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66786.1"
                     /translation="MSLQQLREAAKGHHPGPVLGLYVGTLRYGIEIDDTIRQLAPDRF
                     CATPRYVSLTGQLDRAIHMPREFVDDPCLAALARDHEAKHAVADATAFDHARSPLSSA
                     IRAAVRANTIAASDSRVHALAVLTAGIQTAVDQVFNHMATERRRLDTAVDSPAELEQL
                     KTGCEGRAAVMQ"
BASE COUNT          312 a          473 c          487 g          304 t
ORIGIN      
        1 agctcgtcaa taactggccc aacagccggc tcgcggaact gacgccttgg gcctgggccg
       61 cccaacaccg ctgatcgtca cccccaagta aagcaagccc gtcaaccccc tgcgataatg
      121 gccgaaacgt tgcgcttacg aatgaccgaa aatctccggc cagccgctct cctcgacttt
      181 gttagacgag ttgaccggat ctgctttgat gatcaggaca aagtggctga tcgcgatcct
      241 cgccggcgtg acgccgataa tggtcgcggc gagtgcgcgc ggcggtgatt ccgggtgtcc
      301 tattgctgcg ccggctagag ttcaaatcaa tctcacacgc ggtactgcgg cgcaggtcga
      361 ggcggacatg agcctgcagc agttgcgcga agcggccaag ggccatcacc ccggtccggt
      421 tctcgggctc tacgtcggga cgctgcggta cgggattgag atcgacgaca caattcggca
      481 actagcgccg gatcggttct gcgccacacc taggtatgtg tcgctcaccg ggcaacttga
      541 ccgtgccatt cacatgccgc gggaattcgt tgatgacccc tgccttgcgg cccttgctcg
      601 tgatcacgag gcgaaacacg cggtcgccga cgcgacagcg ttcgaccatg ctcgctcccc
      661 tctctcgtcc gcgatacgcg ccgccgtccg cgctaacacg atcgcagcga gcgattcacg
      721 cgttcacgct ttggccgtgc ttaccgcggg gatccaaact gcggttgatc aggtatttaa
      781 tcatatggcg acagagcgtc gtcgcttgga taccgctgtc gacagcccag ccgaactcga
      841 gcagctgaag accggctgcg agggccgcgc cgcagtgatg caatgactga aatctcatat
      901 tgcggcgggc gatatgctac ttgaacagtg cgaccgggac gccgtgatgt ccaaagtgga
      961 gaacagtcgc gtccacctcg ttcgcgggac catcgatgat cacttggtga gcctgcgtaa
     1021 cgcaagccgg ggtcgaattt tcgccggaag cgggcagctg caatccgcac gaacgctcat
     1081 ccctggcgtg cccgatcgcc agcggcaaaa gccgccacga cgattttcta gagtctcacg
     1141 cgcgcgaggc ggggtttttg gcgcgcagga cggccgcgct tcgatatatt ttcgcgcgct
     1201 atatcagcta attaaggcat gagacgtcat gtcgacatga ccttttagga gggcccaaaa
     1261 ccggcatcga ccggggaaat tcgattttcc ggtcgatttc cgcagcgttt gggaagagct
     1321 cggaacgtgc tcggggcacc ttaaaaaccc tgacgggcgg tctgggcggg cacaggctga
     1381 gctcggcgcg gaggaatgca cacgccgccc gtcctcgttg acgaaattga gcacgctaga
     1441 gcacgatcct gttaagctga agcataaccg gcgttgacga ggtaattggt gcattcctcg
     1501 ggttaacgat cgaggagtga gccgatgccg cgccaggtgg cctcggtggt gcgcggatcg
     1561 gttttgcgca gcgggg
//
LOCUS       DATLJT010001308         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_335504,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001308 DATLJT010000000
VERSION     DATLJT010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335504"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1044..1562)
                     /locus_tag="VM910_30060"
                     /pseudo
     CDS             complement(1044..1562)
                     /locus_tag="VM910_30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            1851..>2212
                     /locus_tag="VM910_30065"
     CDS             1851..>2212
                     /locus_tag="VM910_30065"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HVI66787.1"
                     /translation="MPKAYSGDLRERVIEAVEAGASRHEAAEYFEVSVASAVKWLQRW
                     NKERSAAPKPRGGSVSPLEVFASEILAVVGEQPDLTLDETLVELRKRRIKTSRSSLWR
                     FFDRHNITLKKKPAGGGTP"
BASE COUNT          403 a          661 c          699 g          449 t
ORIGIN      
        1 gacgtgatgc cggccagcca tcgcaacgct cccgtcgggc ccgatctccg cctgcgggcc
       61 ctcacaatgc tcgccacctg ccccaatgga ctcggtcacc gacaacttcc gcggcatctc
      121 gcccactttc gggtcatgac gtgaacgggg tatacctgaa tcctatcgag ctaagaacgc
      181 ggccggcgcg gatatcgcct agcgaaagcc gtcacgcgat acaaacttgg cccttttatc
      241 tcgtcccact acgagtgatt gcgcagcacc atacacgcgc ccccggcgct ccgaatttca
      301 gcacacaggt tttcggcact ttcaagcgta tcggttccga cacggatttg gtagaagacg
      361 ccggtgccgc ggctgtggaa aaccgagctc aagatgctcg cgtctctacc tgccaagatg
      421 ctggcatagc ggctcgcaat cgtcgcatag gccgcaagta cgtgctcgcg cgagaatccg
      481 gcgctgagct gaatgcccca tgagctgtct gcgacaaggt ttactcgctc agtcagcttc
      541 tccacaaaca ggctcgtgtt gccgcgcgcg gacgatggtt cccggtccct cgactcgggg
      601 tctgctcgcc caggaccagg tggcgctgcg acagaatcgt tcttggcgag ctgggtgccg
      661 ggggattgcg gtggatcgtc gcgcgacgac ggcggaggtt cggaagatga agctggttgc
      721 tttgctatgt cgcgggagga tggtgctttc ggcggcatcc caaggtcttc tcgtagagga
      781 atgagtggcc ctgcgagagg accattcttg gcgagctctg tgtcatggga tttcgctggc
      841 tcgtggcgcg acgacggcac aggcttggga ggcgaggctg gttgcttcgc tatgtcgccg
      901 ggggaggacg gtgcttccag tgacgtcaca aggtcttctc gcagaggaac gggtggcgct
      961 gcaatgagat cattcttggc gagctgtgct cgcaccggct cgtcgcgtaa taacggacgc
     1021 ggttcttgag gtgaagctgg tggctggagg agcgccatga gttccccgca attcagactt
     1081 gttttcacgt cgggcttacg agtaccagct ctggcccact catcggcggg aacgccagtg
     1141 attgccagga cgtaattgcg cgtctctgcg gaaagctttc gctcgccggc cagccaagcc
     1201 cgcacccgcg ccggccccgc gttataggca gccgcggcaa gaccaaggct gccgaactga
     1261 tccgtaagct cacgcaggaa ctgagcggcc ttcggcaagg cctgcacggg gtcgagagga
     1321 tcgagcaagc ctctctcagc ggccgtgctc ggcatgaatt gcgcaatgcc ttgggcgctt
     1381 tcgccgttgc gtctgcgggg gcccaccgtg tcaggttgga agcggctttc ctgccagatt
     1441 acccgcacga agaattccgg cggtaaaccg tgggctcgac ctgcagcctc gatcatcaaa
     1501 cagacggaat cctgcaaatc ggatgagtga agatgcgaca ccgcgctgct ttcccgcgcg
     1561 atcgtgggtg ccgccagcgc gctccgtccg atttggcttt tgccttcctt atcctgttgc
     1621 ccacccctcc gcacgttcaa gcgttcctga gtcgcgctgg gactgccgat cgcatccgct
     1681 agctcgcgat cgcggtgccc ctccgctcgc tgggcgacct gcgctgcaac tttgtccttg
     1741 ccaccgccga atgctaaagc ggttgccggc cgatttgaat cggtgggctt cctagagcat
     1801 cagagatgag cgagtcttcg ggtattgatt cgcctttacg gggggctgag atgccgaagg
     1861 cctattctgg tgacctgcgg gaacgcgtga tcgaggccgt cgaggcgggt gcgtcgcgcc
     1921 atgaagccgc cgaatatttc gaggtgagcg tggcgtcggc ggtaaaatgg ctgcagcgct
     1981 ggaataaaga gaggagcgcc gcgccgaaac cccgcggcgg gagcgtctcg ccgctggaag
     2041 tatttgcgtc tgagattttg gctgtggtgg gcgaacaacc tgatctgact ttggacgaga
     2101 cgctcgtaga actgcgcaaa agacggatca agaccagccg tagctcgctt tggcgttttt
     2161 tcgatcgaca caacatcacc cttaaaaaaa agcctgcagg cggcggaacg cc
//
LOCUS       DATLJT010001309         8641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_793049,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001309 DATLJT010000000
VERSION     DATLJT010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793049"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /gene="purH"
                     /locus_tag="VM910_30070"
     CDS             <1..837
                     /gene="purH"
                     /locus_tag="VM910_30070"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438890.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVI66788.1"
                     /translation="YNNINDTDAAYEAVSEFDPKRTAACVIVKHANPCGVAEGKDLVS
                     AYRKALACDSTSAYGGIIALNRTLDTETATAVTEIFTEVIIAPDATDEAIAIVGKRKN
                     LRLLLAGQLPDPRAAGLTAKTVAGGLLVQSRDNAVVDDMALNVVTRRAPTEAELHDLK
                     FAFRVAKHVKSNTIIYAKDLATVGIGAGQMSRVDSARIAARKAQDAANELKLAEPLTR
                     GSVVASDAFFPFADGMLACIEAGATAVIQPGGSMRDDEVIKAADEHGIAMVFTGIRHF
                     RH"
     gene            complement(857..2275)
                     /locus_tag="VM910_30075"
     CDS             complement(857..2275)
                     /locus_tag="VM910_30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66789.1"
                     /translation="MAKRVGVISKDFFVAAPQVDDVAVTTAYPPRSAVISWIFFDWAA
                     QPYFTLITTFVFAPYFATHVAPDAASGQALWGFATAAAGMAIALLSPVLGAIADASGR
                     RKPWIAAFGAMLVIGSALMWFGRPGDVKIIPPLLFAYAIATVGVEFATVFNNAMMPTL
                     VPPDRIGRLSGTGWATGYVGGILSLILVLGFLAASPETGRTLFGFKPLFGLDPLTHQG
                     DRISGPLTAIWFIVFVLPMFLLTPDYPARHPLRVALREGLAELKQTLIELPKHRSLAL
                     FLLANMIYTDGLVSLFAFGGIYAAGTFGWNTIQIGTFGIILAIAGTLGGYFGGKLDDR
                     FGPKRVIAGSMTILLISIIAILAVNKGSILFIPISPPVPGGALFSSSAERAYLVLGCL
                     IGAAGAPLQAASRSLLIRMAPKDRIAQYFGLFALTGKVTSFIGPLLIGIVTAATGSQK
                     AGMATLVLFFATGLVLLARVKK"
     gene            2341..4080
                     /gene="ggt"
                     /locus_tag="VM910_30080"
     CDS             2341..4080
                     /gene="ggt"
                     /locus_tag="VM910_30080"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVI66790.1"
                     /translation="MISLVTNRRNLIAGLALLMAGILPAIALDGRGSIVAQQRPAVST
                     RHGMVVAQEKLAARVGADILRQGGNAVDAAVATAFAMAVTYPRAGNIGGGGFMVIHLK
                     DRGEDIAIDYREAAPAATTAETFLGADGKPDNAKSRDSALSIGVPGTVAGLALALEKY
                     GSGKFTLARLLQPAIVLARDGFVLDDDMADTLPAAQRRMAKWQASVKIFSQADGTWLR
                     GGDRLVQTDLAATLSAIAEQGPRGFYEGPVAEKLVNAVRDAGGIMTNDDLKSYSAVIR
                     KPVRGSYRGYDIVSMPLPSSGGTVLLESLNILEGFPLAEMTQGSPASLHLLIEAMKRA
                     YADRARYLGDPAFVNAPVPVMLSKDYAARQRASIDPDRATASADAAAIKPLREGSNTT
                     HFSVIDDFGNAVSNTYTLNFPYGVGLIAEGTGVLLNNELDDFTAAPGASNAFGLVGFE
                     ANLPGPGKRPLSSMSPTILLKDGKPVLVTGSPGGSRIISTVLQVIVNVLDYHMDVLAA
                     VSAPRLHHQWMPDEVRVERGFSEDVLAQLRAMGHHIVEPMGQTSANSIAVVGDEALGA
                     PDPRTRGAEAAAQ"
     gene            4186..5487
                     /locus_tag="VM910_30085"
     CDS             4186..5487
                     /locus_tag="VM910_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66791.1"
                     /translation="MTTAEPSRAIETATIEDTSLLAFYRDMDVAERRTFWACAAGWAL
                     DGMDFMIYPLVIGTIIALWKVDAGAAGLAGTVTLLSSAVGGWLGGYISDRIGRVRTLQ
                     FTILWFSFFSLVCAFVQNFDQLLLARAVLGLGFGGEWAAGAVLMGEAIRPQYRGRAVG
                     SVQSGWAVGWGLAVLAQAILFSLMPAETAWRWMFAIGALPALLVFYLRRYVTEPEVAA
                     ATRAKQAQTGDRPPLWEIFSPSILKTTILAALMATGCQGGYYAITFWVPRFLTTERKL
                     SIVSSTGYLAALIIGSFAGYLVGAWLADRIGRRNLFLTFSLGAIAVVLLYTQLPLPNG
                     LLWLLGFPLGFFASGYFSGMGAFLTELYPTRLRGSGQGFCYNFGRGIGALFPFLVGAL
                     STTTSLANAIAIFAVAAYAVFFLAAYALPETRGRVLHVEGF"
     gene            5503..6378
                     /locus_tag="VM910_30090"
     CDS             5503..6378
                     /locus_tag="VM910_30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI66792.1"
                     /translation="MTSLKGKTLFITGASRGIGLAIALRAARDGANIAIAAKTTEPHP
                     KLKGTIFTAADDVRAAGGKALPVVCDIRDEAQVIAAVDKTATEFGGIDICVNNASAIS
                     LTDSQATDMKRFDLMMGINTRGTFMVSKYCVPHLKKAENPHILMLSPPLDMKPKWFER
                     STAYSIAKYGMSLCVLGLSAELKSSGIAVNALWPRTTIATAAVGNLLGGDAMMRASRT
                     PEIMGDAAYAIFTRPAREFTGNFCIDDKLLYASGVRDFDRYRVDASVPLMSDFFVPDD
                     DVPPPGVTVRPLPPR"
     gene            6442..7824
                     /locus_tag="VM910_30095"
     CDS             6442..7824
                     /locus_tag="VM910_30095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVI66793.1"
                     /translation="MRKLIVSALILAVVLASCAIYRPDRAIRVATGYVAHNVCSKTFV
                     SGLDPQAVFSEAIDRKGIRTLRAALRYRLDRAARTVDASTLGLFASHAAFREGFGCVL
                     IHGSQPPYLIRSDLEALKAAKTPPLLPEIAGPAAVEPADPALKAALDHAFEEPAEPPF
                     RRTKAVVVVHDGRVIAERYAEGIGVNTPLVGFSMTKSVVSALIGIMTRQGLTSPSLPA
                     PIPEWRASSDPRREIEVGHLLRMTSGLALDETNSGFDPSSQMVFLRDDMASFAVQAPL
                     MAPPGTRWHYSSATTEILAKIIRDAVGGPEQTLAFAWRKLFNPLGMRDVTLEFDAAGT
                     LQGTAYMLASARDWARFGLLYLHDGEVGGKRILHEDWVDYSAAATLDTDYGAGFWTNR
                     SEHRSAVTRVRLGIPRDAFFAAGDLGQRVVIIPSQHLVVVRLGDSTDPTGDVRGFARL
                     VKEVIAATGA"
     gene            complement(7840..8505)
                     /locus_tag="VM910_30100"
     CDS             complement(7840..8505)
                     /locus_tag="VM910_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI66794.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGLPYEIVRYQRGPDMRAPKELR
                     AIHPLGKSPVVTDDGKTIAESGAIVEYLTQTYGDGKLLPRPNTPERLRYTYWLHYAEG
                     SAMPPLLLKLLFTLMPKRAPALLRPLVRKVSSQALTTLVNPQLKQHMAFWEEELAKSD
                     WFAGPEFTAADIQMSFPLEAAAARGGLEQGHPRAMAFLDRIHARPAYQTALQKGGPYA
                     VGR"
BASE COUNT         1558 a         2793 c         2734 g         1556 t
ORIGIN      
        1 tacaacaaca tcaacgacac cgacgcggct tacgaggccg tcagcgagtt cgatccgaag
       61 cgcaccgcgg cctgcgtcat cgtcaagcat gccaatccct gtggcgtcgc cgagggaaaa
      121 gatctcgtca gcgcctatcg caaggcgctc gcctgtgact cgacctcggc ctatggcggc
      181 atcatcgcgt tgaaccggac gctggatacg gagaccgcaa ccgccgtcac cgaaatcttc
      241 accgaagtga tcatcgcacc cgacgcgacg gatgaagcga tcgccattgt cggaaagcgc
      301 aagaatttgc ggctattgct cgccggccag cttcccgacc cgcgcgccgc gggcttgacc
      361 gcaaagaccg ttgccggcgg ccttctggtg cagagccgtg acaatgcggt ggtcgatgac
      421 atggcgctga atgtggtgac cagacgcgcg ccgacggagg ccgagctgca cgacctcaaa
      481 tttgccttcc gcgtcgccaa gcatgtcaaa tccaacacca tcatctatgc gaaagatctc
      541 gcgacggtcg gcatcggcgc cggtcaaatg agccgggtcg attctgcacg catcgccgcg
      601 cggaaagccc aggatgccgc caacgaattg aagcttgccg agccactgac caggggatcc
      661 gtggtcgcct cggatgcgtt ctttccgttc gccgacggca tgctcgcctg catcgaggcc
      721 ggcgccactg ccgtgatcca gccgggcggc tcgatgcgcg acgatgaggt gatcaaggcc
      781 gccgacgagc atggcatcgc catggtgttc accggcatca ggcacttccg gcattgacgc
      841 ttgcgcgtcg cggcaattac ttcttgaccc gcgcgagcag cacgagtccc gtcgcgaaga
      901 acagcaccag cgtcgccatg ccggccttct ggctgccggt cgccgcggtg acgatgccga
      961 tcaacagcgg cccgataaag gacgtcactt ttccggtgag cgcgaacagc ccgaaatatt
     1021 gcgcgatacg gtcctttggc gccatccgga tcaggagcga gcgcgaagcc gcctgcagcg
     1081 gggcgccggc cgcgccgatc aggcaaccga gcacgaggta ggcgcgctcg gcgctggagg
     1141 aaaacagcgc accgcccggc accggcgggc tgatgggaat gaacaggatg gagcccttgt
     1201 tcactgcaag gatcgcgatg atcgagatca acagaatcgt catgctgccg gcgatcacgc
     1261 gcttcggccc gaaacgatcg tcgagcttgc cgccgaagta gcccccgagg gtgccggcga
     1321 tcgccaggat gatgccgaag gtgccgatct gaatcgtgtt ccagccgaaa gtgccggcgg
     1381 catagatgcc gccgaacgcg aacaaggaca caagcccgtc ggtgtagatc atgttggcca
     1441 gcaaaaacag cgcaagcgag cgatgcttcg gcagttcgat cagcgtctgc ttgagttcgg
     1501 cgagcccttc gcgcaatgcc acgcgaagcg ggtgacgcgc cggatagtcg ggggtgagca
     1561 ggaacatcgg cagcacgaag acgatgaacc agatcgccgt gagcggaccg gagatgcggt
     1621 cgccctggtg ggtgagcgga tcgagaccga acagcggttt gaagccgaac agcgtacgac
     1681 cggtttcggg gcttgcggcc agaaagccga gcacgagaat gaggcttaag atgccgccga
     1741 cataaccggt cgcccagccg gtgccggaaa gacgcccgat gcgatcgggc ggcaccaggg
     1801 tcggcatcat cgcgttgttg aacacggtcg cgaattcgac gccgacggtc gcaatcgcgt
     1861 aagcgaacaa cagtggcggg ataatcttta catcgcccgg ccggccgaac cacatcagcg
     1921 cggagccgat caccagcatc gcgccgaacg ctgcaatcca cggcttgcgg cggccgcttg
     1981 catccgcgat cgcacccaga accggcgaca gcaacgcgat cgccatgccg gcggcggcgg
     2041 tggcaaagcc ccacagcgcc tgcccgctcg ccgcatccgg tgcaacatgc gtggcgaaat
     2101 acggcgcaaa aacgaaagtc gtgatcagcg tgaaataggg ctgcgcggcc caatcaaaga
     2161 aaatccagct tatgaccgcc gaacgcgggg gataggccgt agtaacggca acgtcatcca
     2221 cctgcggtgc ggccacaaaa aagtccttcg aaatcacgcc gacgcgtttt gccatttcga
     2281 tccgaccctc tatagcatga tggcgacggt ggattgacga atggcagcgg cggaattttg
     2341 atgatctcgc tcgtgaccaa ccgacgaaac ctgattgcgg gcctggcctt gctgatggcc
     2401 ggcatcttgc cggccatcgc actggacggg cggggttcga tcgtcgcgca gcaacggccc
     2461 gccgtctcca cgcgtcacgg catggtggtg gcgcaggaga agctcgcggc gcgcgtcggc
     2521 gccgatatcc tgcggcaggg cggcaacgcg gtcgacgccg cagtcgccac cgctttcgcg
     2581 atggccgtga cctatccgcg cgccggcaat atcggtggcg gcggtttcat ggtgatccat
     2641 ttgaaggacc gcggcgagga tatcgcgatc gattaccgcg aagccgcacc cgcagcgacc
     2701 acggccgaga ctttcctcgg agccgacggc aagcccgaca atgcgaagtc gcgcgattcc
     2761 gcgctcagca tcggcgtgcc cggtacggtt gccgggcttg cgctcgcgct cgaaaaatat
     2821 ggctccggca agttcactct cgcgcggctc ctgcagcctg ccatcgtgct ggcgcgcgat
     2881 ggctttgtcc tcgatgacga catggccgac accttgcccg ccgcacagcg ccgaatggca
     2941 aagtggcagg catccgtgaa aatcttctcg caggccgatg gcacgtggct gcgtggaggc
     3001 gaccgtctgg tccagaccga cctcgcggcg acgttgtcgg cgatcgccga acaggggccg
     3061 cgcggcttct acgaaggacc ggtcgcggag aagctcgtca acgccgtgcg cgacgccggc
     3121 ggcatcatga ccaatgacga tctgaaatcc tactcggccg tgatccgcaa gccggtgcgc
     3181 ggcagctatc gcggctatga catcgtgtcc atgccgctgc cctcctccgg cggaaccgtg
     3241 ctgctggaat ccctcaacat cctcgaaggc ttcccgcttg ccgagatgac acagggctct
     3301 ccggcgtcgc tgcatctgtt gatcgaggcg atgaaacgcg cctacgccga ccgcgcgcgc
     3361 tatctcggcg atcccgcttt cgtcaatgcg cccgtccccg tgatgctgtc gaaggactat
     3421 gccgccagac agcgcgccag catcgatccc gaccgcgcca ccgcgtccgc cgacgcggcg
     3481 gcgatcaagc cgctgcgcga aggcagcaac acgacgcatt tctcggtgat cgacgatttc
     3541 ggcaacgcgg tcagcaatac ctacacgctg aacttccctt atggggtcgg tctcatcgcc
     3601 gaaggcaccg gtgtgctgct caacaacgag ctcgacgact ttaccgccgc gcccggcgcg
     3661 tcgaacgcct tcggcctggt cggattcgaa gccaacctgc cgggtcccgg aaaacggccg
     3721 ctgtcgtcga tgtcgccgac catcctgctg aaggatggca agccggtcct ggtaacaggt
     3781 tcccccggcg gcagccgtat catctctacc gtgctgcagg tgatcgtgaa cgtgctggac
     3841 tatcacatgg acgttctggc ggcagtgtcg gcgccccggc tgcatcacca gtggatgccc
     3901 gatgaagtgc gggtcgagcg cggtttttcc gaggatgtgc tggcgcagtt gagagcgatg
     3961 ggtcaccaca tcgtcgaacc gatgggacag acctctgcca attccattgc cgtggtcggc
     4021 gacgaggcgc tcggcgcgcc cgatccccgc acccgcggcg cggaagctgc agcgcaatag
     4081 cttttgtccg cattgccagc gccgtgcatc ggagtaggat ttgatcctca tccgagcgcc
     4141 ggctgcggac atagaccgcc ggcaaaaagc ttctggggaa aaaacatgac cacggccgag
     4201 ccatctcggg cgatcgaaac cgccacaata gaagatacca gcctgctcgc gttctatcgc
     4261 gacatggatg tcgccgagcg gcggacgttc tgggcttgcg ccgcgggttg ggcgctcgac
     4321 ggcatggatt tcatgatcta tccgctcgtg atcggcacca tcatcgcgct ctggaaggtc
     4381 gatgccggcg ctgccggcct tgccggcacg gtaacgctat tgtcgtcggc ggtcggcggc
     4441 tggcttggcg gttatatctc cgaccgcatc ggccgggtca ggacgctgca gttcacgatc
     4501 ctctggttct cgttcttttc gctggtctgc gccttcgtgc agaatttcga tcagctcctg
     4561 cttgcgcgag cggttttggg cctcggcttc gggggcgaat gggcggccgg cgccgtgctg
     4621 atgggcgaag cgatccgacc gcaatatcgc ggacgcgcgg tcggttccgt gcaatcgggc
     4681 tgggcggtcg gctggggcct ggcggtgttg gcgcaggcga tcctgttttc gctgatgccg
     4741 gcagaaacgg catggcgctg gatgttcgcg atcggcgcgt tgccggcgct tctggtgttc
     4801 tacctccgcc gatacgtcac cgagcccgag gtcgccgcag cgacccgggc caagcaggcc
     4861 caaaccggcg acaggccgcc gctctgggag attttctcgc cttcgattct caagaccacc
     4921 atcctcgccg cgctgatggc aacgggatgc cagggtggct actacgccat caccttctgg
     4981 gtgccgcgct tcctcaccac cgagcgaaaa ctctcgatcg tgtcctccac cggctacctc
     5041 gccgcgctga tcatcggctc cttcgccggc tatctggtcg gcgcctggct tgccgaccgc
     5101 atcggccggc gcaatttgtt cctgaccttc tcactcggcg cgattgccgt cgtcctgctc
     5161 tatacccaac tgccgctgcc gaacgggttg ctctggctgc tcggcttccc gctcggcttc
     5221 ttcgcctcgg gttatttctc agggatgggt gcgttcctca ccgaactcta tccgacgcgc
     5281 ctgcgcggct ccggacaggg tttttgctat aatttcggcc gcggcatcgg agcactgttc
     5341 ccgttcctgg tcggcgcgct ctcgaccacg acctcgctcg ccaatgcgat tgcgatcttc
     5401 gctgtcgcgg cttacgccgt gtttttcctc gccgcctacg cgctgccgga gacgcgcggc
     5461 cgcgtgctgc acgtcgaggg cttctgaaac ggaggttctg cgatgacatc gctcaagggc
     5521 aagacgttgt tcatcaccgg tgcgagccgg gggatcgggc ttgcgattgc gctgcgcgcc
     5581 gcgcgcgacg gcgccaatat cgcaatcgcc gcaaaaacca ccgagccgca cccgaagctg
     5641 aagggcacca tcttcaccgc cgccgacgac gttcgtgccg ccggcggcaa ggcgctgccg
     5701 gtcgtctgcg acattcgcga cgaagcgcag gtgatcgcgg cagtggacaa gacggccacg
     5761 gagttcggcg gcatcgatat ttgcgtcaac aacgcgagcg cgatcagcct gaccgactcg
     5821 caggccaccg acatgaagcg gttcgacctg atgatgggga tcaatacccg cggcaccttc
     5881 atggtgtcga aatattgcgt cccgcatctg aagaaggccg agaacccgca tatcctgatg
     5941 ctgtcgccgc cgctcgatat gaagccgaaa tggttcgagc gctcgaccgc ctattcgatc
     6001 gcgaaatacg gcatgagcct gtgcgtgctc ggcctctccg ccgagctgaa gagctcggga
     6061 atcgcagtca atgcgctgtg gccacgcacg acgattgcga ccgccgcggt cggcaatctc
     6121 ctgggtggag acgcgatgat gcgcgcgagc cgcacgcccg agatcatggg cgatgcggct
     6181 tacgccattt tcaccaggcc ggcgcgtgag ttcaccggaa atttctgcat cgacgacaag
     6241 ctgctctatg cgagcggcgt gcgcgatttc gaccgctatc gcgtcgatgc atccgtgccg
     6301 ctgatgtcgg acttctttgt gcctgacgat gacgtgccac cgcccggcgt cacggtacgt
     6361 cccctgcccc cgcgctaacg gcgatcaaga cctgcgccaa cggccttgga taacgaagct
     6421 tcctgcagcc aaggagcaac tttgcgaaag ctgatcgtca gcgcgctgat tctggccgtg
     6481 gtgcttgcgt catgcgcgat ataccggccc gatcgcgcca ttcgcgttgc caccggctat
     6541 gtcgcgcaca atgtgtgcag caagaccttc gtctccggac tcgatccgca ggccgtcttt
     6601 tcggaagcca tcgatcgcaa ggggattcgc accttgcgcg cagccctgcg gtatcggctc
     6661 gatcgcgcgg cgcgaacggt cgatgcttcg acgcttgggc tgttcgcaag ccatgctgct
     6721 tttcgcgaag gctttggctg cgtcctgatc catggatcgc agccgccata tctgatcaga
     6781 agcgatcttg aggcgctgaa agcggcaaag actccgccgc tattacccga aattgcgggt
     6841 ccggccgcgg tcgagccggc cgatcccgcg ctcaaggccg ccctcgatca cgctttcgag
     6901 gagccggccg agccgccgtt tcgcaggacg aaggcggtgg tcgtggtaca cgacggccgc
     6961 gtgatcgcgg agcgctatgc cgagggtatc ggcgtcaaca cacccctggt cggcttctcc
     7021 atgacaaagt ccgtggtgag cgcgctgatc ggcatcatga cgcgacaggg gctcacctcc
     7081 ccttccctgc cggcgccgat cccggaatgg cgggcctcat ccgatccgcg ccgggagatc
     7141 gaagtcgggc atctcctgcg catgacgtcg gggcttgcgc tcgatgagac aaattccggg
     7201 ttcgatccct caagccagat ggtgtttctc agggacgaca tggcgagctt tgccgtgcag
     7261 gcgccgctga tggcgccgcc cggcacgcgc tggcactact cgagcgctac gaccgaaatc
     7321 cttgcgaaaa tcatccgcga tgccgtcggc ggccccgagc agacgctggc ctttgcgtgg
     7381 cgcaaactgt tcaacccgct cggcatgcgc gacgtgactc tggagttcga cgccgcgggg
     7441 acgctgcagg gcaccgccta catgctggcg agtgcacgcg actgggcgcg cttcggcttg
     7501 ctctatcttc acgacggcga ggtcggagga aagcgcatcc tgcacgaaga ttgggtcgac
     7561 tattccgccg cagcaaccct cgacaccgat tatggcgcag gcttctggac caatcgcagc
     7621 gaacatcgat cggcggtgac ccgcgttcgc ttggggattc cccgagatgc attctttgcg
     7681 gccggcgacc tcggccagcg cgtcgtgatc atcccctcgc agcaccttgt ggtggttcgc
     7741 cttggcgatt ccaccgaccc caccggcgat gtcagagggt ttgcgcggct cgtcaaagag
     7801 gtgatcgcgg cgaccggcgc atgaaggccg gacgctcaac taacgcccga ccgcgtaagg
     7861 cccgcccttc tggagcgcgg tttgataggc cgggcgcgcg tgaatgcgat cgagaaacgc
     7921 catcgccctg ggatgcccct gctccagccc gccgcgcgcc gcggccgctt ccaggggaaa
     7981 actcatctgg atgtcggctg cggtgaactc cggcccggcg aaccagtcgc tttttgccag
     8041 ctcctcctcc cagaacgcca tgtgctgctt gagctgcgga ttgaccagcg tggtcagcgc
     8101 ttgcgacgag actttccgca ccagcggccg cagcagcgcc ggcgcgcgct tgggcatcag
     8161 cgtgaacagc agcttcaaaa gcagcggcgg catggcggag ccttccgcat agtgcagcca
     8221 ataggtgtag cgcagccgct ccggcgtgtt cggccgcggc agcagcttcc cgtcgccata
     8281 ggtctgagtg aggtattcga cgatcgcgcc tgattcggcg atggtcttgc cgtcgtctgt
     8341 gacgacaggc gatttgccga gcggatggat cgctcgcaat tctttgggcg cgcgcatgtc
     8401 gggcccgcgc tgatagcgca cgatctcgta gggcaagccg agctcttcga gcagccacag
     8461 cacccgctgc gagcgtgaat tgttgagatg atgaacggtc agcattggat gtcccccggc
     8521 aatcaatggg cgatccccta acaggccgcc gcaggattgg cgatgggcgg aacatttatt
     8581 tattacccgg gtaatattga cgtgcgaata ttgcccgggt attaatcgat cagaatggat
     8641 t
//
LOCUS       DATLJT010001310         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1944649,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001310 DATLJT010000000
VERSION     DATLJT010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944649"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VM910_30105"
     CDS             <1..531
                     /locus_tag="VM910_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI66795.1"
                     /translation="SYNKGDKDFNAIVSRLKLENIDLVYVGGYHQEAGLILRQMRDQG
                     LKTVLMAGDAMADKEYASITGPAGEGTLFTFGPDPRKKPTAAAIVEKFKAKNIDPEGY
                     TLYTYAAMQIWSQAAAKAGTTDPKKVMDTIKAGAWDTVIGKIEYDAKGDIKQLDYVVY
                     KWDDKGGYAEVSPKGS"
     gene            751..>3441
                     /locus_tag="VM910_30110"
     CDS             751..>3441
                     /locus_tag="VM910_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI66796.1"
                     /translation="MKTQTLSDRNIFLSTLPASERDRNLALAVVGVSALLFAFAVPYA
                     GVPLIKVPAFVASYQSALAVNDLITAVLLFSQFGVLRAWSLLLLASGYLFTAAAAVIH
                     ALTFPGLFAPTGLLGAGPQTTVWLFMIWHGGFPLLALGYAFTNGKDGGVRVSWPPGRA
                     ILASIAAVAVAMTAATLLVTRGHDALPILLSEGHYTAWLTGVVSFVWCLSLAALLVLL
                     FRRPHSVLDVWLMVVLCAWLFDIALAAVVNVGRFDLGFYAGRIYGLCAASFVLAVLLF
                     DNVALQAQMARMVRSLRRRAAVERDHHTGRERLFSAVVESSNDAIITESLDGAVTAWN
                     KAAERLFGFTSTEAVGKHIRLIVPLGRRDEMYDFIERIARGETIENCETRRLHKDGRE
                     LDVIVSMSPLRSASGAIIGASKIARDITESKRTQKALSQEIEERQRIFETSQDLILVT
                     DTAGNFVQVSPSAKTILGYSPEEMIGRSAIDFIHPDDLDSTREEMRSARRGQQMRNFE
                     ARYRHKDGRSVMLNWMGTWSEPVRRHFFIGRDLTEKHAAEAQIRQAQKMDAVGQLTGG
                     VAHDFNNILTVITGTIGIIADAVADRPDLASIARLIDDAAERGAQLTKHLLAFARKQP
                     LQPREVDVNALVLEAAKLLHPTLGAQVEICPKLSEDVWSALVDPNQLTTAILNLALNA
                     RDAMSAGGKLTLETRNVYLDEGYTGINSEVAVGHYVMIAVSDTGSGIAPENLDKVFDP
                     FFTTKEVGKGTGLGLSMVFGFVKQSGGHIKIYSEIGHGTSVKIYLPRSIGTQDSATET
                     GAAPTVEGGNEVILVVEDDLLVRKYVLTQIASLGYSTLEAANATEALKIIDDGAAIDL
                     LFTDVIMPGDMNGRQLADEATKRRPQLKTLFTSGYTENAIL"
BASE COUNT          647 a         1127 c         1059 g          608 t
ORIGIN      
        1 tcctacaaca agggcgacaa ggactttaac gccatcgtct cgcggttgaa gctcgagaac
       61 atcgatctcg tctatgtcgg cggctaccac caggaggccg gcctgatcct gcgccagatg
      121 cgcgatcagg gcttgaagac cgtcctgatg gccggcgacg cgatggccga caaggaatat
      181 gcttcgatca cgggacctgc cggcgaaggc acgctgttca ccttcgggcc cgacccgcgc
      241 aagaagccga ccgctgccgc gatcgtagag aagttcaagg cgaagaacat cgatcccgag
      301 ggttatacgc tctacaccta cgccgcgatg cagatctggt cgcaggctgc ggcaaaagcc
      361 ggcaccaccg atccgaagaa ggtcatggac accatcaagg ccggcgcctg ggacaccgtg
      421 atcggcaaga tcgagtatga cgcgaagggc gacatcaagc agctcgatta cgtcgtctac
      481 aaatgggacg acaagggcgg ctatgccgaa gtcagcccca agggttcctg atcgtcggaa
      541 tgggctgcca gccaatcgga cgccccggct cgccggggcg tttcgttttt tgagtatgca
      601 tccgatcgag tcggtcgatc ctccgcttgg agcctgcttc tagcgccccg atgcgaacca
      661 agtatgatgt aaccgcccct ggattccgca aagattgtcg tggaacctga ggactagcgc
      721 gcgatccggt tctcgtgcgt gggggtagcc gtgaaaacgc agacgctgag cgaccgcaac
      781 atcttcctgt ccacgctgcc ggcgagcgag cgcgaccgga acctggccct tgccgtcgtc
      841 ggcgtctccg ccttgctgtt tgcgttcgcc gtcccctatg caggggttcc gctcatcaag
      901 gttcccgcct ttgtcgcgag ctatcagtcg gcgctcgcgg tcaacgacct catcaccgcg
      961 gtgctgctgt tctcgcaatt cggcgtgctg cgtgcatggt cgctgctcct gctcgccagc
     1021 ggctatctgt tcacagccgc ggcggccgtc atacatgccc tcacctttcc cgggctcttc
     1081 gctccaaccg gacttctggg agccggcccg cagaccacag tctggctgtt catgatctgg
     1141 cacggcggct ttcccctact ggcgctcgga tatgccttca ccaacggcaa ggacggcggg
     1201 gtcagggtct cgtggccgcc cggacgggcc atcctggcaa gcattgccgc cgtcgccgtc
     1261 gccatgaccg ccgccaccct gctcgtcacc aggggacatg acgcgctgcc gatactgctc
     1321 agcgaaggtc attacacggc ctggctgacc ggcgtcgtca gcttcgtgtg gtgcctcagc
     1381 ctcgcggccc tgctcgtgct gctgtttcgc cgtccccatt cggtgctcga cgtctggctg
     1441 atggtcgtgc tgtgcgcctg gctgttcgac atcgcgctgg ccgcggtggt caacgtcggg
     1501 cgcttcgacc tcggcttcta tgccgggcgg atctacgggc tctgcgcggc aagtttcgtg
     1561 ctggcggttc tcctgttcga caatgtcgcg ctgcaggcgc aaatggcccg catggtgcgt
     1621 tcgctgcgcc gccgggccgc cgtcgagcgc gaccatcaca ccgggcgcga acgcctgttc
     1681 agcgcggtgg tggagtcctc caacgacgcc atcatcaccg agtcgctcga tggtgccgtc
     1741 accgcctgga acaaggccgc cgaacgcctg ttcggattca cctcgacgga ggcggtcggc
     1801 aagcatatcc gcctgatcgt tccgcttggg cgccgcgatg aaatgtacga ttttatcgag
     1861 cggatcgcac ggggcgagac catcgagaac tgcgagaccc ggcggctgca caaggacggc
     1921 cgcgagctcg acgtaatcgt cagcatgtct ccgctccgct cggcttcggg cgcgatcatc
     1981 ggcgcatcaa aaatcgcgcg cgacatcacc gagagcaagc ggacgcaaaa ggcgctgagc
     2041 caggagatcg aggaacggca gcgcatcttc gaaacctcgc aggacctcat cctcgtcacc
     2101 gataccgcgg ggaatttcgt gcaggtgagc cccagcgcga agaccattct gggctatagc
     2161 cccgaagaga tgatcggaag aagcgcgatc gacttcatcc accccgacga tctcgacagc
     2221 acgcgcgagg agatgcgctc ggcgcggcgc ggccagcaga tgcgcaattt cgaggcgcgc
     2281 tatcgccaca aggacgggcg cagcgtcatg ctgaactgga tggggacatg gtcggagccg
     2341 gtgcgccgcc atttcttcat cgggcgcgac ctgaccgaga aacatgccgc ggaggcgcaa
     2401 atccggcagg cgcagaaaat ggacgccgtc ggccagttga ccggcggcgt cgcccacgac
     2461 ttcaacaaca tcctgaccgt gattaccggc acgatcggca tcatcgccga tgccgtcgcc
     2521 gatcgccccg accttgcctc gatcgccaga ctgatcgacg acgcggccga gcgcggcgcg
     2581 caactgacca agcatctgct cgcctttgcc cgcaagcagc cgctgcagcc gcgcgaggtc
     2641 gacgtcaatg ccctggtgct ggaggctgcc aagctgttgc acccgacgct gggagcgcag
     2701 gtcgagatct gtccgaaact gtccgaggat gtctggtcgg cgctggtcga tcccaaccaa
     2761 ctcaccaccg cgatcctgaa tcttgccttg aacgcgcgcg atgcgatgtc ggccggcggc
     2821 aagctcacgc tcgagacgcg caacgtctat ctcgacgagg gctataccgg cataaacagc
     2881 gaggtcgccg tcggccacta cgtcatgatc gcagtgagcg ataccggcag cgggatcgca
     2941 ccggaaaatc tcgacaaggt gttcgatcct ttcttcacta ccaaggaggt cggcaaaggg
     3001 accggccttg gactctccat ggtgttcggc ttcgtcaagc agtccggcgg ccacatcaag
     3061 atctacagcg agatcggcca cggcacgtcc gtgaagatct acctgccgcg ctcgatcgga
     3121 acgcaggact ccgcaaccga gacgggcgcg gccccaaccg tcgaaggcgg caacgaggtc
     3181 atcctcgtcg tcgaggacga tctcctggtg cgcaaatatg tgctcaccca gatcgcaagc
     3241 ctcggctatt ccacgctgga ggcggccaac gcgaccgagg cgctgaagat catcgatgac
     3301 ggcgcggcca tcgatctgtt gttcaccgac gtcatcatgc ccggcgatat gaacggccgc
     3361 cagttggcgg acgaggcaac gaaacgccgt ccgcagctca agacgctgtt cacatcggga
     3421 tacaccgaaa acgccatctt g
//
LOCUS       DATLJT010001311         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3783003,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001311 DATLJT010000000
VERSION     DATLJT010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3783003"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..493
                     /locus_tag="VM910_30115"
     CDS             260..493
                     /locus_tag="VM910_30115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66797.1"
                     /translation="MRRRDFITLFGGTAAWPLAARAAADVPVIGFLGAATPAVASQWL
                     AAFVQRLGLPNVRQFGDTSISAATLSQRGRLMH"
     gene            complement(559..891)
                     /locus_tag="VM910_30120"
     CDS             complement(559..891)
                     /locus_tag="VM910_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI66798.1"
                     /translation="MLRQALPIALITVAALAPLGAHADEKLTLKSVNVDLPFGDRMFP
                     DGPGSEAVNNNCLACHSAGMVLTQPALSKEQWHAEVEKMRTAYKAPIDPKDVETIVNY
                     LVTIQRAK"
     gene            complement(902..2134)
                     /locus_tag="VM910_30125"
     CDS             complement(902..2134)
                     /locus_tag="VM910_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI66799.1"
                     /translation="MQTLSLRSLASRRRFLGTAGAATLTLSPLTALADAMIRLPLPGG
                     PDERSITTAFPQKGALILQRTRPPLLETPLEVFDQSVFTPNDKFFVRWHWANIPSTVD
                     VNSFRLAVHGHVSQSLSLSLDDLMAFPRVELVAVNQCSGNSRGYFQPRVSGGQWSHGA
                     MGNARWTGVRLKDVLDRAGVKAGAVQVRFNGMDEPVVPDAPDFKKSLDIEHARNAEVM
                     LAYAMNGEQLPLLNGFPLRLIVPGWYSTYWVKMLNDVEVLDQPDANFWMKTAYTVPDT
                     PHANVQPGQTGFKSVPISRMVPRSFITNIKPGDSLQARAPGLARGIAFGGDTGVARLD
                     FSSDGGKTWQQAQLGKDEGKYSFRQWQIRFTAPERGSTVLMVRCTSSSGETQPNTPNW
                     NPSGFMRHVIEQTPVVVS"
BASE COUNT          581 a          862 c          930 g          618 t
ORIGIN      
        1 cgcttctcct tctgatgctt gaggccgcat atgcggacct cgtttcacca tcagcctgaa
       61 gcgcagcacc ctatttttca gctatctaca agctggcagg ccgattaccc catctttcgg
      121 gggtaaagcg gacataggct agggacggcc tgaggtctgc ttatgaccca aagcggacat
      181 aagcgggtcg cttttgctgc gatgcacggg cctgacctgc tatacttagc ccgtgatccc
      241 tggccttggg gcacgcccca tgcggcggcg cgacttcatc accctgttcg gtggcacggc
      301 agcatggccg ctcgcggcgc gcgcagcagc agacgtgccg gtgatcgggt tcttgggtgc
      361 tgctacgcct gcggttgcga gccaatggct cgccgctttt gtgcagcgac tgggtttgcc
      421 caatgttcgg caattcggag atacgtctat ttcagcggca acgttaagcc agcgcgggcg
      481 gctgatgcac tgattcagat caaacgagcc gccggtgcgc cgcgatgtga tggcagatgc
      541 cccggaaaat ggatactttc atttcgctcg ctggatggtg acgagatagt tgacgatcgt
      601 ctcgacgtcc ttgggatcga tcggcgcctt gtaagccgtg cgcatcttct cgacctcggc
      661 gtgccattgc tccttcgata gcgccggctg cgtgagcacc atgccggcgg agtgacaggc
      721 caggcaattg ttgttgaccg cctccgaacc ggggccgtcc gggaacatgc gatcgccgaa
      781 gggcagatcg acgttaaccg acttcagcgt caatttttcg tcggcatggg cgcccagcgg
      841 cgcaagcgca gcgactgtga tgagcgcgat aggcagcgcc tggcgcaaca tgttcccctc
      901 cttaggatac gaccacgggc gtctgctcga tgacgtggcg catgaacccg cttggattcc
      961 aattgggtgt gttgggctgc gtctcaccgc tgctgctggt gcatcggacc atcagcacgg
     1021 tggatcccct ctccggcgcg gtgaatcgga tctgccactg gcggaagctg tacttgcctt
     1081 cgtccttgcc gagttgggct tgctgccagg tcttaccgcc gtcgctggag aaatcgaggc
     1141 gggccacgcc tgtatcgccg ccgaacgcga tgccgcgtgc gagccccggg gcgcgtgcct
     1201 gtagcgaatc gcccggcttg atgttggtga tgaaggagcg cggaaccatc cggctgatcg
     1261 ggaccgattt gaagccggtt tggccgggct ggacgttggc gtggggggta tcggggaccg
     1321 tgtaagcggt cttcatccag aaatttgcgt cgggttggtc gagcacctcg acatcattga
     1381 gcatcttaac ccagtaggtg gaataccagc cgggtacaat gaggcggagc gggaagccat
     1441 tgagcaacgg gagttgctcc ccgttcatgg cataggcgag catgacctcg gcgttccgag
     1501 catgctcgat gtcgagcgac tttttgaagt cgggcgcgtc cggcacgacc ggctcgtcca
     1561 tgccgttgaa gcgaacttgg acagcgccgg ccttcacgcc cgcgcgatcg agcacgtcct
     1621 tcagtctgac tccagtccag cgggcgtttc ccatggcgcc gtgcgaccac tggccaccgg
     1681 acacgcgggg ctggaaatag ccgcgcgaat tgcccgagca ttgattgacg gcaacaagtt
     1741 ccacccgcgg gaacgccatc aggtcatcga gcgacagcga caggctctgg ctcacgtggc
     1801 cgtgcaccgc caaccgaaag ctgttcacgt cgaccgtgct tggaatattc gcccaatgcc
     1861 agcgcacgaa gaacttgtcg ttcggggtga acaccgattg gtcgaacacc tcgagcggcg
     1921 tttccagcag cggcggtcgg gtgcgttgga ggatgagcgc acccttctgc ggaaaggccg
     1981 tggtgatcga tcgctcgtcc ggtcctccgg gcaagggaag ccggatcatt gcatcggcaa
     2041 gcgcggtcag cggcgaaagt gtcagggttg cggcgccggc cgtgccgaga aagcgtcggc
     2101 gggatgcgag ggatcgaaga gagagcgttt gcatgtgcgt actccgactg agctccgcct
     2161 ggattcggcc ccttgcgttt taccccggtc cgacggaatg ggctgtcgcg cgattgccct
     2221 ggttgggcga tcttgcgcca atttccggtg atgctcacgc tccttgacaa ggtgatcgaa
     2281 cagcaggtgc tttttgccgc ggcgcattag gacgtgaccg gcacaaagct cacatcccga
     2341 gatgtctgcc gacttgtccg ctttcaaaag ggtgaaacag acaacggcga gtaccagtgc
     2401 acgcgatcag ccggtgggcc cattgaaggg aggcaagcat tagctccagg ctgccgcaat
     2461 tatccaaacg ccggaactac gaagagtgca ttggagcaaa gaagcatcta gttttgggtt
     2521 tgtcgtccca tggcccaaag gtgggatcaa cggcatttgc ccatggagga tcgatacaac
     2581 ggtggaaacg atcatcaggc gatggtgttg ccatatcggc aggaataatt tcatcagtcg
     2641 tcggtagata gtaattgcca tctggcagca ctgtagcctg cactggctta caatcgtgcc
     2701 ccccacagca tagatgtcca tcccgatcac ggagattgct gtagatgtca tgggctagga
     2761 cgctagtcga aaggcaaagt aggacaatcg ctgccaacaa gtctttcatc tgagcccccg
     2821 tgctctttag actcatttcc catcatcgct acgtgaaaat atcgcagccg cgctgtcctg
     2881 tgcttccagc aagtgcgtga acttccacga tccaaagcat tgaagaaagc agcaccaata
     2941 ctccacatgc acaggccacg tgtgcctgag ttagtgtggc cgcactgaaa c
//
LOCUS       DATLJT010001312         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1715934,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001312 DATLJT010000000
VERSION     DATLJT010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715934"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VM910_30130"
     CDS             complement(<1..141)
                     /locus_tag="VM910_30130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HVI66800.1"
                     /translation="MPSGRVKFFNPDRGYGFIAPNDGGPDIFVHVHDVEVAGMKILEA
                     GQL"
     gene            complement(404..>2600)
                     /locus_tag="VM910_30135"
     CDS             complement(404..>2600)
                     /locus_tag="VM910_30135"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI66801.1"
                     /translation="VTLCASYDAVILLRDGDDLRIAAHHGPMRLDFERIQLGRDTVSG
                     RTVLDGMPVHVHDLTAEAGEFPRGRDIAVRLNQRTVLGLPLLREGQAMGCLFLRRIEV
                     LPFSDKQIALLQTFAAQAVIAIENTRLFNELRQRTDDLSESLQQQTATADVLKVISHS
                     TFDLQTVLDTLVQSAAQLCEAETAFVFQRGGEVYRLAANHGFSPEYEQFIRRNPIVPG
                     RGTLTGRTAFEGRTIHIPDVLADPEYTWSESQKLGGFRTWLGVPLLREGVPIGVLALS
                     RSTPRPFSDKQIELVTTFADQAVIAIENVRLFDEVRARTRELAQSVEELQALGEVSHA
                     VNSTLDLETVLATIVAKAVQLSNTDAGVIYVFDELDQTLRVRATYGLSDELVAAIRGQ
                     PAGASDALRRAIQDRQSLEIADIRDEPPSPVREIAMRAGFRARLVVPLVGTNRVVGAL
                     VIRRKQPGNFPKGTIDLLQTFAAQSVLAIQNARLFRDIEDKSRQLESASEHKSQFLAS
                     MSHELRTPLNAIIGLTEMMVSNAARFGTEKALEPLRRVNAAGTHLLSLINEILDLSKI
                     EAGKLELNPERVNLARLIDEVIGTAGQLAEKNQNRLVVEAQDNLGALTADSMRLKQIL
                     LNLLSNACKFTKEGEVALRVRKVADGRNWVELAVTDTGIGMTAEQQAKLFQDFAQADS
                     LTARRYGGTGLGLAISRKLARMMGGDVTVTSEPGKGSVFTVRLPGGDCT"
BASE COUNT          497 a          822 c          783 g          498 t
ORIGIN      
        1 caattggcct gcttccagta tcttcatgcc ggcgacctcc acgtcgtgga catgcacaaa
       61 aatatccggg ccgccgtcat tgggcgcgat aaagccatat ccgcgatctg ggttgaagaa
      121 tttgacccgc ccacttggca tcccatttaa tccgacctca actcacagta accgccccga
      181 cccgccccga aggagagcaa cggcgacgga ccggggcttg gcacacgcgc tgaggttgtt
      241 cggcactaag cgcttcatgc ccgaaagagt tataccccaa aagcggcttg gccgcaggtc
      301 gcctaattca ctgcttcgac cgcatatcgg cggatggtct gatcgccttg tcacggtccg
      361 ggctaaggtc agtcgaatcg acggcgtggc aggtgcatgg ggttcatgtg caatcgcccc
      421 ccggcagccg caccgtaaat accgatcctt tgcccggctc gctcgtgacg gtcacgtcgc
      481 cgcccatcat gcgcgcgagc ttgcgcgaga tggcgaggcc gagcccagtg ccgccgtagc
      541 gccgcgccgt gagtgaatcg gcctgggcga aatcctgaaa cagtttcgcc tgctgctccg
      601 ccgtcatacc tatgccggta tccgtgacgg caagctcgac ccagttgcgt ccgtccgcca
      661 ccttgcgcac ccgcagcgcg acctcgccct ctttcgtgaa cttgcaggca ttgctcagaa
      721 gattgagcag aatctgcttg agccgcatgg aatccgcggt gagcgcgccc aggttgtctt
      781 gcgcttcgac gacgaggcgg ttctggttct tctcggcaag ctgtcccgcg gtgccgatga
      841 cctcatcgat gaggcgggcc aaattcactc gctcgggatt gagttcgagc ttgccggctt
      901 cgatcttcga gaggtcgagt atctcgttga tgaggctcag gaggtgggtc ccggcggcat
      961 tgacgcggcg cagcggttcc agtgcctttt ccgtcccgaa gcgcgccgcg ttgctgacca
     1021 tcatctcggt taggccgatg atggcgttca acggcgtgcg cagctcgtgg ctcatgctgg
     1081 caaggaactg cgatttgtgc tcactggctg actcgagctg gcggctcttg tcctcgatgt
     1141 cgcggaacag gcgcgcgttc tggatggcca gcaccgattg cgccgcgaac gtctgcagga
     1201 gatcaatggt acccttggga aagttgcccg gctgcttgcg ccggatcacg agcgccccga
     1261 cgacacgatt tgtgccgacc agcggcacga cgaggcgcgc ccgaaatccg gcccgcatcg
     1321 cgatctctcg gaccgggctt ggcggctcgt cgcggatatc tgcgatctcc agtgattgtc
     1381 ggtcctggat cgcccggcgg agcgcatcgg aagcaccggc tggctggcct cggattgccg
     1441 caaccaactc atcgctcaag ccatacgtcg cccgcacccg caatgtctgg tccagctcat
     1501 caaagacata gatcacccca gcatccgtat tcgaaagctg gaccgccttg gcgacgatcg
     1561 tggccagcac ggtctcgaga tcgagggtgg agttgaccgc gtggctgacc tcgccgagcg
     1621 cctgcagctc ttcgaccgac tgcgcgagtt cgcgtgtgcg cgcccgcacc tcgtcgaaca
     1681 gccgcacgtt ctcgatcgcg atcaccgcct ggtcggcgaa ggtcgtcacc agctcgatct
     1741 gcttgtcgga gaacggccgt ggcgtcgagc gcgacaatgc cagcacgccg atgggaacgc
     1801 cctcgcgcag cagagggacg ccgagccaag tccgaaagcc gccgagtttt tgagattcgg
     1861 accaagtgta ttcagggtcc gcgaggacat ccggaatatg gattgttctg ccttcgaacg
     1921 cggttcgtcc ggtgagcgtg ccacgaccag ggacgatcgg attgcgcctg atgaactgct
     1981 catattctgg cgagaatcca tggttcgcag caagacgata gacctcgccg ccacgctgga
     2041 aaacaaacgc agtctcggcc tcgcacagct gcgccgctga ctgcaccaac gtatcgagca
     2101 ccgtctgaag atcaaaggtc gaatggctaa tgaccttgag cacatcggcg gtggcggtct
     2161 gttgctgcaa cgattcagag agatcgtcgg tgcgctggcg caattcgtta aacagtcgcg
     2221 tgttttcaat agcaatgact gcctgggccg cgaaggtctg gagcagcgca atctgcttgt
     2281 cggaaaaagg tagcacctcg attcgacgca ggaacagaca gcccatagcc tgcccctcgc
     2341 gcaatagggg cagaccgagc accgttcgct gattgagccg caccgcgata tcacggcccc
     2401 ttgggaactc cccagcctcg gcggtgagat cgtgcacatg aaccggcatt ccgtcgagca
     2461 cagtgcggcc agacaccgta tcgcgcccga gctgtattcg ctcaaaatcg agtctcatgg
     2521 ggccgtgatg cgccgcgatc ctgaggtcgt cgccgtcccg caacagaatc acagcatcat
     2581 aagacgcaca aagcgtgacc
//
LOCUS       DATLJT010001313         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3783388,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001313 DATLJT010000000
VERSION     DATLJT010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3783388"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VM910_30140"
     CDS             <1..868
                     /locus_tag="VM910_30140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="negative regulator of septation ring formation"
                     /protein_id="HVI66802.1"
                     /translation="EEADAMFKQSADRFASLVTSMKQMAAEMHHELETTRSELRRGVL
                     EMPQEAAESTAQMRKVIVDQIEALAELNRIVAHHGRGLDVVGANRGGVQRQEETVIAT
                     AAGGRAEPRMRDAGSASNLPPPDLGVPSPRRADAPPMSPATADQGRDGWLSDLLSRAD
                     NAQPSAPRGRSPQPGNGNPLESLSLDINRLMDRNLAAEMWDRYQRGESKAFTKRLYTP
                     AGQKAFDEVARKYRADRAFKQTVDRYITEFERLLDEVAREDRGPQALRGHLTSETGLV
                     YTLLAHAAGRLG"
     gene            complement(979..1866)
                     /locus_tag="VM910_30145"
     CDS             complement(979..1866)
                     /locus_tag="VM910_30145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVI66803.1"
                     /translation="METRANYVLIGSFTLAVIAAAFGFVLWFQSLHTTKARSPLRIVF
                     EGPAAGLRNGGSVNFNGIRVGEVISVKLDNPRRVVALAMVENNAPIRKDTLVGLEFQG
                     LTGVAAISLKGGEEAAPPVPLDEDGVPVLTADPNRLQDVTEAIRGTLQNINRVVADNQ
                     EAVKNSLRNLETFTNSLSRNAERIDAIMGRVDGVMGKADNLMLGLNSLAGGKDGGELF
                     LTVKSIRELAEDFDKRSGALMADGRRTLSDISRAVNNFDRNPTRVLFGASNSSQPTQA
                     QGQAPAPAAAPAATERKRQ"
     gene            complement(1972..2742)
                     /locus_tag="VM910_30150"
     CDS             complement(1972..2742)
                     /locus_tag="VM910_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66804.1"
                     /translation="MAPTPSDAIIRVRDITVQFGSTRVLNGLDLDVKRGEILGFVGPS
                     GAGKSVLTRTIIGLVPKVAGRIEVFGVDLDAADPAARRAVERRWGILFQQGALFSSLT
                     VRQNIQFPVREYLKVSQRLLDEIMVAKLGMVGLRPEVADRFPSELSGGMIKRVALARA
                     LALDPELVFLDEPTSGLDPIGAGDFDELVRTLQRTLGLTVFMVTHDLDSLYTACDRIA
                     VLGNGKIIAAGSMADMQASQHPWLRQYFHGKRARAVMG"
     gene            complement(2745..>2963)
                     /locus_tag="VM910_30155"
     CDS             complement(2745..>2963)
                     /locus_tag="VM910_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI66805.1"
                     /translation="RDAISIDHFTVGLIKAPVMAAVIGIVACVEGLAVQGSAESLGQH
                     TTASVVKGIFFVIVMDGVFAIFFASIGM"
BASE COUNT          564 a          970 c          933 g          496 t
ORIGIN      
        1 ggaagaagcg gatgcgatgt tcaagcagtc cgcggacagg ttcgcgagcc tggtgacgag
       61 catgaagcag atggcggccg aaatgcatca cgagctcgag acgacccgca gcgagctgcg
      121 ccgtggcgtg ctcgagatgc cacaggaggc cgccgagagc accgcgcaga tgcgcaaggt
      181 gatcgtcgac cagatcgagg cgctggcgga gctgaaccgg atcgtcgccc atcacggtcg
      241 cggtctcgac gtggtcggag ccaatcgcgg cggcgttcag cgtcaggaag agaccgtcat
      301 agcgaccgcc gccggcggtc gcgccgagcc gcgcatgcgt gatgccggca gcgcgtcgaa
      361 cctgccgccg ccagacctcg gcgtgccgag ccctcgccgt gccgatgccc caccgatgag
      421 ccctgcaacc gcggaccagg gacgcgacgg ctggctctcg gatctgttga gccgggccga
      481 caacgcacag cccagcgccc cgcgcggtcg ctcaccccag ccgggcaacg gcaatccgct
      541 ggagtcgctt tcgctcgaca tcaaccggtt gatggaccgc aatctcgctg ccgaaatgtg
      601 ggaccgctat cagcgtggtg agagcaaggc gttcaccaaa cgtctctaca cgccggccgg
      661 ccagaaggcg ttcgacgagg tcgcccgcaa ataccgcgcc gaccgtgcct tcaagcagac
      721 ggtcgatcgt tacatcaccg agttcgagcg gttgctggac gaagtggcgc gcgaagaccg
      781 cgggccacag gcgctgcggg gccacctcac ctcggagacg ggtctcgtct ataccctgct
      841 cgcccacgct gcaggtcggc tgggataagc ggcaaatagc tgatgacgaa ttgaaaaacg
      901 gaggcctcgc ggcctccgtt tggttctggg ccaagcggtg cagccccgcg cgctactggt
      961 tgtccaccat ccataatctc attgccgctt cctctcggtc gcagcgggcg ctgccgccgg
     1021 cgccggtgcc tggccctgtg cctgggtcgg ttggctcgaa ttgctcgcac caaacagaac
     1081 cctggtcggg ttgcggtcaa aattgttcac ggcgcggctg atgtcggata gggtcctgcg
     1141 gccgtctgcc atcaacgcgc ctgagcgctt gtcaaaatcc tccgctagct cgcggatcga
     1201 tttcacggtc aggaacagct caccgccatc cttgccgccg gcgagcgaat taagcccgag
     1261 catcagattg tcggccttgc ccatcacgcc atcgacccgc cccataatcg cgtcgatccg
     1321 ctcggcattg cgggagagcg agttggtgaa ggtttccagg ttacgcagcg agttcttcac
     1381 cgcttcctga ttgtcggcga ccaccctgtt gatgttttgc agcgtgccgc ggattgcctc
     1441 ggtgacgtcc tgcagccggt tcggatctgc ggtcagcacg ggcacgccgt cctcgtccag
     1501 cggaaccggc ggcgctgcct cctcgccgcc cttcagcgag atcgcggcaa cgcccgtgag
     1561 accctggaat tcgaggccga cgagggtgtc cttgcggatc ggtgcgttgt tctcgaccat
     1621 cgcgagcgcc accacccgac gcgggttgtc gagcttcacc gaaatgacct cgcctaccct
     1681 gataccgttg aaattcacac taccgccgtt gcgcaaacca gccgcggggc cctcgaaaac
     1741 gatgcgcaga gggctgcgcg ccttggtggt gtgcaggctc tggaaccaca gcacgaagcc
     1801 gaacgcggcc gcgatcaccg ccagcgtgaa tgatccgatc aggacgtaat tcgcccgcgt
     1861 ttccatcgag aactccggtt actgcacgcc gacgcagctt cggcgccgtc gcgagtttct
     1921 gcgctggcgc atgatccttt ccgaaaaccg cctgacgctt tgaatcatgc gctaccccat
     1981 cacggcgcgg gcacgcttac cgtgaaaata ttgacgcagc cagggatgct gcgaagcctg
     2041 catgtcggcc atcgatcccg cggcaataat cttgccgttc cctaagacgg caatacggtc
     2101 gcaagcggta taaaggctgt ccaagtcgtg ggttaccatg aaaacggtca ggcccaaagt
     2161 gcgctgcagg gttctcacca gctcgtcgaa atcgccggcg ccgatcggat caaggccgga
     2221 agttggctca tccaggaaca cgagttccgg atcgagcgcg agggcgcgcg ccagcgccac
     2281 gcgtttgatc atgccgccag acaattccga cggaaagcgg tcggcgacct cgggccgcag
     2341 gccgaccata ccgagcttgg cgaccatgat ctcgtcgagc agccgttgcg agaccttgag
     2401 atattcgcgg accggaaact ggatgttttg ccgcacggtc agcgaggaaa acagggcgcc
     2461 ctgctggaac aggatgcccc atcgccgctc cactgcgcgc cgggccgcag gatcggcggc
     2521 atcaagatcg acgccgaaca cctcgatgcg cccggcaact ttgggcacca gcccgatgat
     2581 ggtgcgtgtc agaaccgatt tgcccgcccc ggagggtccc acgaacccca ggatctcgcc
     2641 gcgcttgacg tcgaggtcga gtccgttgag cacgcgggtc gacccgaact gaacggtgat
     2701 gtcgcgaacc cggatgatgg cgtcggaggg agttggagcc atgatcacat cccgatcgac
     2761 gcaaaaaaga tggcaaacac gccgtccatc acgatcacga agaaaatgcc cttcaccacg
     2821 gaagcggtcg tgtgctggcc gagcgattcc gcgctgcctt gcaccgcgag gccctcgacg
     2881 caggcgacga tgccgattac cgccgccatg acaggggcct tgatcaggcc gacggtgaag
     2941 tgatcgatcg agatcgcgtc gcg
//
LOCUS       DATLJT010001314         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1716320,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001314 DATLJT010000000
VERSION     DATLJT010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716320"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VM910_30160"
     CDS             <1..1879
                     /locus_tag="VM910_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVI66806.1"
                     /translation="ATSWLINRDALRQAVEAQIRAVTGLELTVKGPIDVSVFPGSYVS
                     FHDVGLKGGAASDPALRVDVLTANLRLLPLLLQRFEIADLMMLRPQIHVARRADGESN
                     WTPFIETIARTMKPGADNQVSFSEIRIQDGTLDYEDAGNHASEKLEDIDLSLAWPSIS
                     RSFAATGQFNWRGERVDGSISISDFVAALSGDRSGLKARLASAPLKLAFDGTVANRTS
                     AMMEGTLTVDTASLRNALQWTGQAPPAGGGFGRFALKARANVVGASIALTNVNVELDG
                     NVAEGVMTYSNNGRQTLQATLAADALDFTPYISTFRLLASGARDWNRQLFDLNSLLTT
                     DLDMRLSAARVTVGPTKLGRTAFGANLRNGALALSVGEAQVYGGIAKGSFGVARSDAI
                     ADVKAQFQFLDVDLQACASELFGVNRLSGRGNLNVSLVASGSSPFGLASSLDGTATLT
                     GHDGAISGFNVEQLLKRLERRPLSGGNIRSGSTPFDNLTVSLKFAEGVATTEEVRVEG
                     PASRVTLTGTASVPTREYNLKGVASLTSNGATSFELPFVVEGPWDDPLIYPDPESLIR
                     RSPATAPLLDAVKDKKARDAVRSVLERFTGGGVRPGAPDTAAPAPAPSPAAASEAAKS
                     N"
     gene            2087..3472
                     /locus_tag="VM910_30165"
     CDS             2087..3472
                     /locus_tag="VM910_30165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66807.1"
                     /translation="MRSKGLGAISIAMAAIGLLYAAAPAMAQQKTITVWFGKGFYKSE
                     DDALLEAIKKFEAKTGIKVELSQYAIQDMIPKTVAALDSGTVPDVAYSDSYDVQAQGK
                     WAYEGKLEDLSDILLPMKSAFAPNTLETTYLYNDVAKKKGYYGFPLKQQSMHVQIWQD
                     MLEQAGFKQSDIPTKWEDYWSFWCDKVQPAIRKATGQRIYAVGQPMGVESTDSFQSFY
                     TFMDAYKVKLVDDDGKLLVDDPKVRDGLIHALKDYTDTYIKGCTPPSSTTWKDPDNNV
                     AFHNRTIVMTHNFTISIAAKWLDDSNNPALTPEQRAAGKKAYEETIITASFPKAPDGS
                     TIKYRSDVKTGLIFAAAKNKAEGKEFIKFLLQEENVRPYIEGALGRWFPVTTASQQSP
                     FWQADRHRKAVYNQFMGGTTPFDFTKNWKFTILNNENVWAKAMNRVVSEKVPVDKAVD
                     ELIARIKEVAG"
     gene            3596..>3841
                     /locus_tag="VM910_30170"
     CDS             3596..>3841
                     /locus_tag="VM910_30170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI66808.1"
                     /translation="MAITLSASDVPGAPLSSRLSPSQVWGIVLLAPYILVFLAFVLYP
                     VGYGLWLARQPSSYVTLFHDPIFARAGVNTLIFLLVGI"
BASE COUNT          758 a         1244 c         1164 g          675 t
ORIGIN      
        1 cgccacctcc tggctgatca accgcgacgc gttgcgccag gccgtggaag cgcaaatccg
       61 cgccgtgacc gggctcgaac tgacggtcaa aggcccgatc gacgtttcgg tcttccccgg
      121 aagctatgtc tcctttcacg acgtggggtt gaaaggcggt gccgcctccg acccggcgct
      181 gcgcgtcgat gtcctcaccg caaacctgcg gttgctgccg ttgctgctgc aacggttcga
      241 gatcgccgat ttgatgatgc tgcgtccgca gatccacgtc gcacgccggg ccgacggcga
      301 gagcaactgg acgccattca tcgaaacgat cgcgcgcacc atgaagcccg gcgccgacaa
      361 ccaggtttcg ttctcggaaa tccgcatcca ggacggtacg ctcgattacg aggacgccgg
      421 caaccatgct tccgaaaagc tcgaggacat cgacctctcg ctcgcctggc cctcgatctc
      481 gcgttcgttt gccgcgaccg gccaattcaa ctggcgcggt gaacgtgtcg acggctcgat
      541 cagcatctcc gacttcgtcg ccgccctgtc gggcgatcgc tccgggttga aggcgcggct
      601 tgccagcgcg ccgctcaagc tcgccttcga cggtacggtc gccaatcgca ccagcgcgat
      661 gatggaaggc acgctgacgg tcgataccgc ctcgctgcgc aacgcgctgc agtggaccgg
      721 acaggcgccg cccgccggcg gcgggttcgg ccgcttcgcg ctgaaagcgc gcgccaatgt
      781 cgtcggcgct tcgatcgcgc tgaccaacgt caatgtcgag ctcgacggca acgtcgcaga
      841 aggcgtcatg acctattcga acaacggccg gcagacgctg caggcgacgc tggccgccga
      901 cgcgctcgac ttcacgccct acatctccac cttccggctg ctcgccagcg gtgcgcgcga
      961 ttggaaccgg caactgttcg acctcaactc gctcttgacc accgacctcg acatgcgcct
     1021 gtcggcggcg cgcgtgactg tcggcccgac gaaactcggc cgcaccgcat tcggcgcaaa
     1081 tctgcgcaac ggcgcgctgg cattgtcggt cggcgaggcg caggtctacg gcggaatcgc
     1141 caagggctcg ttcggcgtcg cgcgatccga cgcaatcgcg gacgtgaagg cgcagttcca
     1201 gttcctcgac gtcgatctgc aggcctgcgc cagcgagctg ttcggcgtca accgcctgtc
     1261 cggccgcggc aatctcaacg tttcgctggt ggcgtcgggt tcgagcccgt tcggattagc
     1321 ctcatcgctg gacggcaccg cgacgctgac cgggcatgac ggcgcgatct ccggcttcaa
     1381 tgtcgagcaa ctcctgaagc ggctggaacg ccggccactg tcgggcggca atattcgcag
     1441 cggttccacg ccattcgaca atctgacggt ctcgctgaaa tttgccgaag gcgtcgccac
     1501 caccgaggaa gtccgcgtgg agggaccggc ctcacgcgtg acgctgaccg gcacggcgtc
     1561 ggtgcccacg cgcgaatata acctcaaggg cgttgccagc ctgacatcga acggcgcaac
     1621 gagtttcgaa ctgcccttcg tggtggaggg gccctgggac gatcccctga tctatccgga
     1681 tccggaaagc ctgatccggc gctcgcccgc gaccgcgccg cttctcgacg ccgtcaagga
     1741 caagaaggcg cgcgacgcgg tgcgctcggt gctcgagcgc ttcaccggcg gcggcgtacg
     1801 gccgggcgcg cccgatactg ccgcgccggc accggctcct tcgccggccg ccgcatccga
     1861 ggccgccaaa tcgaactagc gtcccgattt tcggacttcg cattgcgaac gcgagtgcgg
     1921 cacgccaggc gtaagtcgct ctgagcaaaa gcgcatcgcg ccgcgagcga catcaggcac
     1981 cgcgcctcga cttacgtcgg acaagatgtc gctagatcgt ctcgcggcgc tgcgctagtg
     2041 tttcagccaa ccggtaaaaa gatcggcgag acacagggag aaagacgtga gatccaaggg
     2101 tttaggtgca atttcaatcg cgatggcggc tatcggcctg ctttacgccg cagcccccgc
     2161 gatggcccag caaaagacca ttacggtctg gtttggcaaa ggcttctaca agtccgagga
     2221 cgacgcgctg ctcgaggcga tcaagaaatt cgaggccaag acggggatca aggtcgagct
     2281 gtcgcaatac gcgatccagg acatgatccc gaagacggtc gcagcgctcg attccggcac
     2341 ggtgccagac gtcgcctatt ccgacagcta tgacgtgcag gcccagggca agtgggctta
     2401 tgaaggcaag ctcgaggatc tctccgacat cctgctgccg atgaagtcgg cctttgcgcc
     2461 gaacacgctg gaaacgacct acctctataa cgacgtcgcc aaaaagaaag gctattacgg
     2521 ctttccgctg aagcagcaga gcatgcatgt gcaaatctgg caggacatgc tggaacaggc
     2581 cggcttcaag caaagcgaca tcccgaccaa atgggaggat tactggtcgt tctggtgcga
     2641 caaggtgcag cccgcgatcc gcaaggcgac gggtcagcgc atctacgcag tcggccagcc
     2701 gatgggcgtc gaatcgaccg actcgttcca gtcgttctac accttcatgg acgcctataa
     2761 ggtcaaactg gtcgacgatg acggcaagct tctggtggac gaccccaagg tgcgcgacgg
     2821 cctgatccac gcgctgaaag actatactga tacctacatc aagggctgca cgccgccctc
     2881 ctccaccacg tggaaggacc cggacaacaa cgtcgccttc cacaaccgga cgatcgtgat
     2941 gacgcataac ttcacgatct cgatcgcggc gaagtggctt gatgactcca acaacccggc
     3001 gctgacgccg gaacagcgcg cggccggcaa gaaggcctat gaggagacga tcatcaccgc
     3061 ctccttcccg aaggcgccgg atggctcgac catcaaatac cgctccgatg tcaagaccgg
     3121 gttgatcttc gcggctgcca aaaacaaggc cgagggcaag gagttcataa aattcctgct
     3181 gcaggaagag aacgttaggc cctacatcga aggcgcgctc ggccgctggt tcccggtaac
     3241 gaccgcgagc cagcagagcc cgttctggca ggctgaccgc catcgcaaag cggtctacaa
     3301 tcagttcatg ggcggcacca cgcccttcga cttcaccaag aactggaagt tcaccatcct
     3361 aaacaacgag aatgtttggg ccaaggcgat gaatcgcgtc gtgagcgaga aggtgccggt
     3421 ggacaaggcg gtcgacgaat tgatcgcccg catcaaggaa gtcgcgggtt gatgcaacgc
     3481 atgatccgga aaaaaggagt atccttgttc cgatcggatc atgcgtaaaa cgtaaataag
     3541 aggatcggtc gctctgtttg tcggccggtc cttcctggcg taaagtcaaa gtgccatggc
     3601 gattaccctg tcagcttccg atgtcccggg cgcgccgctg tcttcgcggc tttcgccatc
     3661 gcaggtgtgg ggcatcgtgc tgctcgcacc ctacatcctg gtgttcctgg ccttcgtgct
     3721 ctacccggtc ggctacgggc tatggctggc gcgccagccg tcaagctatg tcaccctgtt
     3781 ccacgatccg atcttcgcac gcgcgggggt caacacgctg atcttccttt tggtcggcat
     3841 c
//
LOCUS       DATLJT010001315         4826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4241877,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001315 DATLJT010000000
VERSION     DATLJT010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4241877"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VM910_30175"
     CDS             complement(<1..797)
                     /locus_tag="VM910_30175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI66809.1"
                     /translation="MSRAVQLIPKLEYLVTLGTVLRFQRRPQEAFEAFDKAAQLKPDA
                     VELWRSRGDLLLELKRPAEALLSFQRVLQLNPQHWEAALKSGVLLHEEGRLEEALSCF
                     NLCDELQPNQAPTLRSRGRSLSVLGRLNEAMADYMRAHALDSSDAANCANIGGILQSL
                     DRNDEALPWLDEALRLQPDLPDALRNKAVWLRDHRRFDEAFAIYERLKALDPGNAKAE
                     WNLSHLYLLTGNLEAGWAAREARWKVPGLPLARFEPPQPIWLGQECI"
     gene            852..986
                     /locus_tag="VM910_30180"
     CDS             852..986
                     /locus_tag="VM910_30180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66810.1"
                     /translation="MKGVCAIRVYREGLLATELRIKKSFGSQMIEPGLAKRGWAGDIA
                     "
     gene            complement(1297..2346)
                     /locus_tag="VM910_30185"
     CDS             complement(1297..2346)
                     /locus_tag="VM910_30185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tlde1 domain-containing protein"
                     /protein_id="HVI66811.1"
                     /translation="MACLVLGSSWTVYSNIIAANVYPTLEAAGYDEPVVRRPKLALRS
                     TAQAISDAFSALPEAPVIEQPETVPAITPTMFNERFAASQAQGVESRAADAPAAPGLD
                     APKLAEAPKPAEAPKIAAVSKSKGAAPAQVALNTPPQATPRAADAKADTPSGLSMRDM
                     AQRAKAAVMSIASAERLSITEKLWGKEAASHGSLLSYASADASITGSVGHEQSPTLGG
                     SPPYERDTAVYDISAHTVYLPDGTKLEAHSGLGSKLDDPKSASIRMHGVTPPHMYTLT
                     PRESLFHGVAALRLNPVGGEDAIYGRAGLLAHTYMLGPNGDSNGCVSFKDYNAFLNAY
                     RNQGIKKLAVVARID"
     gene            2777..3595
                     /locus_tag="VM910_30190"
     CDS             2777..3595
                     /locus_tag="VM910_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVI66812.1"
                     /translation="MQSVGRKLAFVLASSNHGSMIVNRFDCRMLDGQRGYGVGFQILE
                     TASFDAAEVKLAVDLLAIRRKHHGDGVVAIDCGANIGVHTIEWAKAMTGWGSVLAIEA
                     QERIYYALAGNIAINNCFNAIAVHAAVSSESGVLPIPNPNYLVPSSFGSLELRRRSGN
                     EFIGQPIDYDNTVNVRKLMLDEYQLQRVDLIKLDIEGMELEALAGAQQTIERCRPIML
                     IEKIKADAAQLRQWLQLSGYGIIEAGINLLAIHAADGSLAEIAPRAQQHEPTPA"
     gene            complement(3599..>4826)
                     /locus_tag="VM910_30195"
     CDS             complement(3599..>4826)
                     /locus_tag="VM910_30195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVI66813.1"
                     /translation="AAILFGGTRLAANATILFNVTLMVAVGTLIVTPDSPLLVAASFV
                     LFFLAKVLETGRGAWWLAVGAAVGCALLSKYTALLFGPAILIWLVAVPKQRSWLISPW
                     PYLGGLVAFAIFASVILWNAEHQWVSFLKQIGDRVRVDEFHPYHVAELIPTQFAFATP
                     LVFILGAMGLYALIRRKAGAMPARVLIDAMFWTIAAYFIWHSLHARVEANWFAPVYPA
                     FAIAAAVAAHLVRWEAREERLVRFCLRWAPSVGIVMFALLVLQANTGWLSLYRRDATV
                     RSVGVGWRELADQIEAVRKRVGATCVLAPDYGTTSWLAFYLPRSTCVVQPIQRIRWVN
                     MGEPDRAQLAGPLLYVDEVRPEATPYVKNTFARVARIVELPRRRGPLTIETYAIDLLQ
                     EPKGDVLDRSPPPELQ"
BASE COUNT          946 a         1507 c         1504 g          869 t
ORIGIN      
        1 tcaatgcact cctgccccaa ccagatcggt tgaggcggtt cgaagcgcgc aagcggcaga
       61 cccggaacct tccaccgggc ctcgcgggct gcccagccag cctccaaatt gccggtcagc
      121 aggtaaagat gggaaaggtt ccactcggct ttcgcattgc cgggatcaag agccttcagg
      181 cgctcataga tcgcgaaagc ttcgtcgaag cgacgatgat cccggagcca taccgccttg
      241 ttcctcaatg cgtccggaag atccggctga agacggagcg cttcgtccag ccacggcaga
      301 gcctcgtcat tgcgatccag cgactgcaga attccgccga tgttggcgca gttggcggca
      361 tcactggaat cgagcgcatg ggctcgcatg tagtcggcca ttgcctcgtt gagccgaccg
      421 agaacgctga gggagcggcc gcgcgaacgc agggtcggag cttggttcgg ctgcagttcg
      481 tcgcacagat tgaagcatga cagcgcctcc tccagccgtc cctcctcatg gaggagaacc
      541 ccgctcttga gagcggcctc ccagtgttgc ggatttagct ggagtacgcg ctgaaagctc
      601 agcagcgctt cggccggccg cttgagctcc agaaggaggt cgccacggct tctccatagc
      661 tcgacagcat ccggcttgag ctgcgccgcc ttgtcgaacg cctcgaaggc ctcttgcggc
      721 cgacgctgaa accgcaagac ggttccgaga gtcacgagat attcaagctt cggaattagt
      781 tgcacagcgc ggctcaccca tgccaccgcg tgatcgtatt gttgggtgtg gatcgacagc
      841 aggcccatca gatgaagggc gtctgcgcaa ttcgggtcta tcgcgagggc ttgctggcaa
      901 cagagttgcg catcaagaaa tcgttcggct cgcagatgat tgagcccggt ctcgcaaagc
      961 gcggctgggc tggtgatatc gcgtgaagct atccaggatt tttcggccgc agagcgatct
     1021 tcacggtgag tcacttgcgc aatccatctc aagccagcaa tgtgtgtgtg actaaacgat
     1081 tcggtgatta tctccaaggt tgcatattct tatgagcgcg cgcgctttca catgcgcgcc
     1141 actcattcgt cccgcgcatc gacttgatgg atcgatactt cagacgcctt gagcgtcgcg
     1201 aagacatacg cgccgcggct tttgaatccg aatgagaaag gtcgaccgct tcgccgatcg
     1261 tcacatgcat gcaggccttg cgcgcgtttg cgccgctcaa tcgatcctgg cgacgaccgc
     1321 gagcttcttg atgccctggt tgcggtaggc gttgaggaag gcgttatagt ccttgaacga
     1381 aacgcagccg ttggaatcgc cgttcggccc gagcatgtag gtgtgcgcca gcaatccggc
     1441 gcggccgtaa atcgcgtcct cgccgccgac cggattaagt cgcagcgccg ccacgccgtg
     1501 gaacagcgac tcgcgcggcg tcagggtgta catgtgcggc ggtgtcacgc catgcatcct
     1561 gatcgacgcc gatttgggat cgtcgagctt cgagccaagg ccggaatgtg cttccagctt
     1621 ggtgccgtca ggcaaataga ccgtgtgcgc ggaaatatcg tagaccgcgg tatcgcgctc
     1681 gtagggcggc gagccgccga gcgttgggct ctgctcgtgg ccgacgctgc cggtgatgct
     1741 ggcatcggcc gaagcatacg acaacagcga gccgtgcgac gccgcctcct tgccccacag
     1801 cttctcggtg atcgagagcc tttcggccga ggcaatcgac atcaccgccg ccttcgcccg
     1861 ctgcgccata tcgcgcatcg aaaggcccga gggggtgtcc gcctttgcgt cggcggcgcg
     1921 cggcgtggcc tggggcggcg tgttcaatgc gacctgcgcc ggcgcggcgc ccttcgattt
     1981 cgataccgcg gcaatttttg gcgcttcagc aggttttggc gcctcggcga gcttcggcgc
     2041 atcaagcccc ggcgcagccg gtgcgtccgc agcgcgcgac tccacgcctt gcgcctggga
     2101 cgctgcaaac ctttcgttaa acatagttgg cgtgatcgcg gggaccgtct ccggttgctc
     2161 gatcacgggt gcctcgggca gggccgaaaa tgcgtcgctg atggcttggg ccgtcgagcg
     2221 gagggcaagc tttggacgcc tcacgacggg ctcgtcatag cccgcggcct ccagcgtcgg
     2281 ataaacgttc gctgcgatga tgttggagta gacggtccag ctcgagccca gcaccagaca
     2341 cgccattccc accccgccga gcagagtgtg agagatggtt ttcccagggg attttctgcg
     2401 gcttcccgcc gccaacgcta cgctactcgt actcattcac acgcgcccag gactgctcca
     2461 ctcttcctct cgcaacgcca aaggggctca ctaatgcagc gtctcgcaga ggggcgaagc
     2521 ttcattaagg cgacttttag ttaaatgcgg attaagtccg aggcggatgt aaggcgaccg
     2581 aatatgttcg gaatacggtt tcatttcggc acaacggacc gtggaaaatc cgggaagcga
     2641 tcgccatctg ccgcgtcgga ttcatgacca aatatgggcg ttgccatggc cggacgcgcc
     2701 ccggcacagt aagcttgccg atcaagcgat tcgtattgcg gtctgtcgct tatctcgaat
     2761 ctttcaggag atcccgatgc agagcgtagg gcgcaagctg gcgttcgtgc tggcctcatc
     2821 caatcacggc tccatgatcg tcaaccgctt cgattgccgc atgctcgacg ggcagcgcgg
     2881 ctatggtgtc ggcttccaga tcctggagac cgcgagcttc gatgcggccg aggtgaagct
     2941 cgcagtcgac ctgcttgcca tccgccgcaa gcatcatggg gacggcgtgg tggcgatcga
     3001 ttgcggcgcc aatatcggcg ttcataccat cgaatgggcg aaggcgatga cgggctgggg
     3061 atcggtgctc gcgatcgaag cgcaggagcg catctactac gcactcgccg gcaacatcgc
     3121 aatcaacaac tgcttcaatg ccattgcggt gcacgccgcc gtgtcgtccg agagcggggt
     3181 tctgccaatt cccaatccga attatctcgt gccctccagc ttcggcagcc tcgagttgcg
     3241 ccggcgaagc ggcaacgagt tcatcggcca gcccatcgac tatgacaaca ccgtcaatgt
     3301 ccgaaaattg atgctcgacg aatatcagct ccagcgcgtc gatctcatca agctcgacat
     3361 cgaaggcatg gagcttgagg cgcttgccgg cgcgcagcag accatcgagc gatgccgccc
     3421 gatcatgctg atcgaaaaga tcaaggcgga cgccgcgcaa ttgcgccaat ggctgcaact
     3481 atccggctac ggcatcatcg aggccgggat caacctgctc gccatccatg ccgccgacgg
     3541 ctcgctcgcc gagatcgcgc cgcgggcgca acagcacgaa ccgacaccgg cgtgagcctc
     3601 attgcaattc cggcggcggc gatcggtcga gcacgtcgcc tttcggctcc tgcaggagat
     3661 cgatcgcgta ggtctcgatc gtgagcggac cgcgcctgcg gggcagctcg acgatgcgcg
     3721 cgacccgggc gaacgtgttt tttacatacg gcgtcgcctc cggcctgacc tcgtcgacat
     3781 acagcagcgg cccggcaagc tgcgcacggt cgggctcacc catgttgacc cagcggatgc
     3841 gctggatcgg ctgcaccacg caggtcgagc gcggcaggta gaaggcaagc cagctcgtgg
     3901 tgccgtaatc aggcgcaagc acgcaagttg cgccgacacg cttgcgcacc gcttcgattt
     3961 gatcggccag ttcgcgccac ccgacgccga cactgcgcac ggtcgcatcg cgccggtaaa
     4021 gcgaaagcca gccggtgttg gcctgcagga cgagaagggc aaacatgaca ataccgaccg
     4081 agggcgccca gcgcaggcag aaacggacca ggcgctcttc gcgcgcctcc cagcgcacca
     4141 gatgcgcggc caccgcggca gcgatcgcga atgccggata gacaggtgcg aaccagttcg
     4201 cctcgacccg cgcatgcagg gaatgccaga tgaaataggc cgcaatggtc cagaacatcg
     4261 cgtcgatcag gacccgcgcc ggcatcgcgc cggccttgcg gcggatcagc gcgtacagtc
     4321 ccatcgcgcc caggatgaac accaggggcg ttgcgaacgc gaactgggtc gggatgagtt
     4381 cggcgacatg gtagggatga aactcatcga ccctcacgcg gtcgccgatc tgcttgagaa
     4441 acgataccca ttgatgctcg gcattccaga gaatgaccga cgcgaagatc gcaaacgcca
     4501 cgaggccgcc gagatagggc cagggcgaga tcaaccagct ccgttgtttt ggcacggcca
     4561 cgagccagat caggatcgcg ggaccgaaca ggagcgcagt gtatttcgac agcagcgcgc
     4621 atccaaccgc cgctcccacc gcgagccacc acgcgccacg gccggtctcg agaactttgg
     4681 cgagaaaaaa caggacgaag ctcgcagcaa cgagcagcgg cgaatccggt gtgacgatca
     4741 gcgtgccgac ggccaccatc agcgtgacgt tgaacaggat ggttgcgttg gccgcaaggc
     4801 gcgtgccgcc gaagaggatc gcggcc
//
LOCUS       DATLJT010001316         4053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1945279,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001316 DATLJT010000000
VERSION     DATLJT010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945279"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..433
                     /locus_tag="VM910_30200"
     CDS             137..433
                     /locus_tag="VM910_30200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HVI66814.1"
                     /translation="MQSRGVLLSLLQNRSCPKCHTRQTKDWLDARTTEVLPTYYFHVT
                     ITVPQGLRALLRTNQRDGYALLMKAAADAIIELAHAPVRRRHRRRAGCSPECRR"
     gene            complement(671..2728)
                     /locus_tag="VM910_30205"
     CDS             complement(671..2728)
                     /locus_tag="VM910_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI66815.1"
                     /translation="MSKITAEHLQRNACVYIRQSTPDQIVHNLESQRRQYGLADRAKQ
                     LGWTTVEIIDDDLGRSGGGIARPGFERLLAAICDGRVGAVLAIEASRLARNGRDWHTL
                     IEFCGLVGTILVDEDGIYDPRHPNDRLLLGMKGTMSELELSLFRQRSQEALRQMARRG
                     ALVLGVAAGYVKVGRDRIEKNPDKRVQQAIQLVFTKFADLQSARQVHIWLREEGIELP
                     AKSRRGEAHGVVWRLPAYNIVHNILTNPIYAGAYAFGRTTSRISVVDGRKRVRRGVHR
                     PMGEWDVLIKDHHAAYISWDEFERNLKAIANNATGMSSALTRGAAREGELLLPGLLRC
                     GHCGRKLHVHYGGKLGRYNCYGARVNHGTERCITVSGLSIDAAIAKEVLRVLKPLGMD
                     AAVKAIEAQSSETTAAERQLELSLQQARYEVAHARRQYDAVDPTNRLVAGELERRWNE
                     TLQAVAKIEGDIAAMMARRPPPLGEPERQQLLALGADLECAWLHPAATAATRKRILRT
                     AISEVIVRRDGAIIHAVLHWQGGDHTELQVKQRLNAAGRHNPRIPDDTIALIRQLARL
                     MPDRQIARLLNRIGVATGHGNAWTQKRVRGFRDHYEIAGYREGEWAERGEITLEAAAK
                     IVGVCNMTALRMLRRGDIKGRQVCPGAPWVIKTADLIGFTGRKRLDSPLTPNPDQRAF
                     DFQ"
     gene            complement(2709..2891)
                     /locus_tag="VM910_30210"
     CDS             complement(2709..2891)
                     /locus_tag="VM910_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66816.1"
                     /translation="MAHKSEHHQPGLFDEETPLVKLYPARRVELAGLVEALFREIAAT
                     LVNVKSGGSIHEQDHR"
     gene            complement(3164..3979)
                     /locus_tag="VM910_30215"
     CDS             complement(3164..3979)
                     /locus_tag="VM910_30215"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI66817.1"
                     /translation="MSTVTVSPLRQRMIEDMNARKLCAGTQRGHIHSCRRFAAFLKQS
                     PDTATAEDIRRFQLHLSETGVSICNRNRIMTGLRFLLRVTLRRLDLAAEIYHIREPQK
                     IPLVMSQDETRRLLAVASSLKARTLLSLGYGCGLRAGEVVRLKVKHIDRAQNIIRVEQ
                     SKGRKDRNVMLSAEMLDLLRQWWKVRPSRHDAGTPMQERWLFPGTKRGQTMSTRQLNR
                     LFHQAADAAGIRKGVTLHALRHSFATHLLEHGTDIRKIQAVLGMATYCPRTAS"
BASE COUNT          744 a         1265 c         1235 g          809 t
ORIGIN      
        1 ttatcgccgg gcattgacgc ggtaacccga tccacagaac ttgctttgcc gtcatgtatg
       61 tctgaggatc ggcgaggcgg caacccttgg cttgccgcac cgaggccctc ggcggcatct
      121 gttggcgctg cgatcaatgc agtcacgagg tgttctccta tcactcctgc aaaaccgctc
      181 ctgtcccaaa tgccacacca ggcagaccaa ggattggctc gatgcccgta cgaccgaggt
      241 gctgccgacc tattatttcc acgtcaccat cacggtgccg caagggctgc gagcactgct
      301 gcgaaccaac cagcgtgacg gctacgccct gctgatgaag gctgcggccg acgccatcat
      361 cgagcttgcc cacgccccag ttcgtcggcg gcaccgtcgg cgtgctggct gttctccaga
      421 atgtcggcga tagcacggcg cgaggctggc atcgccgcac catgggctga cagatagccg
      481 tccgcgaacc ggcggaagac gtcggcaagc tcgatcacgg cggctgaccg caatcagagg
      541 tcggtcatca acctgtcgag aaggcttctg tgcgagaccc tggtcggctc ggtcaggtgg
      601 gtgtaaatgc tggtcgtggc aatgttgcta tgcccgagcg gacacgagtc ataatgcacc
      661 tcctcttaac ttattgaaag tcaaaggccc gctgatctgg atttggtgtt agcggggagt
      721 ctaatcgctt gcgaccagta aatccgatta gatccgcggt cttgatcacc cagggagcac
      781 ctgggcagac ctgccggccc ttgatgtcgc cacggcgaag catgcgcagc gccgtcatat
      841 tgcatacccc gacgattttc gccgctgctt cgagcgtgat ctcgccgcgc tcagcccatt
      901 cgccctcgcg gtagcccgca atctcgtagt gatcgcggaa gccgcgcacg cgcttctgcg
      961 tccacgcatt gccatgtccg gtcgcaacgc caatgcgatt gagcaggcgc gcaatctggc
     1021 gatccggcat cagtcgggcc agctgacgta tgagcgcgat cgtgtcgtcc ggaatgcgtg
     1081 ggttgtggcg accggctgca ttcagccgct gcttcacctg cagttcagtg tggtcgccgc
     1141 cttgccagtg caggactgca tgaatgatcg cgccgtcgcg gcgcacgatg acctcggaga
     1201 tagcagtgcg cagaatccgc ttgcgcgttg cagccgtcgc ggcgggatgc aaccaggcgc
     1261 actcgagatc ggcaccgagc gcgaggagtt gctgccgctc cggctcacca agcggcggcg
     1321 gacgccgcgc catcatggcc gcgatgtcgc cttcgatctt ggcaaccgct tgcaaagtct
     1381 cgttccatcg gcgctccagc tcgccggcga cgagccgatt ggttggatca acagcgtcgt
     1441 actgccgacg ggcatgggca acctcatagc gggcctgctg cagggagagt tccagttgcc
     1501 gctcagccgc cgtggtctcg ctcgactggg cttcgatcgc cttgaccgca gcatccatac
     1561 cgagcggctt caggacgcgc agcacctcct tggcgatcgc tgcgtcgatg ctcaagccac
     1621 tcaccgtgat gcatcgctcg gtgccgtggt tcacgcgcgc gccatagcag ttatagcgac
     1681 cgagcttgcc gccgtagtgc acatggagtt tgcggccgca gtggccacag cgcagaagcc
     1741 caggcagcag cagttcgcct tcacgggctg ctccgcgagt gagcgcgctg ctcatgccgg
     1801 ttgcattgtt ggcgattgct ttgagattgc gttcgaactc gtcccagctg atgtaggcgg
     1861 cgtgatgatc cttgatcaga acgtcccatt cacccatcgg ccggtgcacg ccacgacgaa
     1921 cgcgcttgcg gccgtccacc acgctgatcc ggctcgtcgt gcgcccgaag gcataagcgc
     1981 cggcataaat cggattggtc aggatgttgt ggacgatgtt gtaggctggc aagcgccaca
     2041 cgacgccatg tgcctcgccc cggcgcgatt tggcgggcaa ttcgatccct tcttctctga
     2101 gccagatatg cacctgccgc gcgctctgga ggtcggcgaa tttcgtgaag acgagttgaa
     2161 tggcctgttg cacgcgtttg tccggattct tctcgatccg gtcgcgcccg actttcacat
     2221 agcccgcagc cacaccgaga accagcgcgc cacggcgtgc catctgacgc aacgcctcct
     2281 gtgagcgctg gcggaacagc gacaattcaa gttcgctcat cgtccccttc atgccgagca
     2341 acagccgatc gttgggatgg cgaggatcgt agattccgtc ctcgtcgacg aggatggtac
     2401 cgaccaatcc acagaactcg atgagtgtgt gccagtcgcg gccgttgcgg gcgagacgtg
     2461 aagcctcgat cgcaagcacc gcaccgacac gaccgtcaca gattgcggcc aacagtcgct
     2521 cgaagcctgg acgtgcgatg ccgccgcccg atcgtccgag atcgtcgtcg atgatctcga
     2581 cagtcgtcca gccaagctgc ttggcacgat cggcaagccc atattgccgt cgctggcttt
     2641 caagattgtg cacgatctga tcaggagttg attgacggat ataaacgcaa gcgttgcgct
     2701 gtagatgctc agcggtgatc ttgctcatgg atgctccccc cgctttttac gttgaccagc
     2761 gtcgctgcga tctcgcggaa cagagcttcc acgaggcccg cgagctcgac cctgcgagcc
     2821 gggtatagct tcacgagcgg agtctcctcg tcgaacaggc ctggttggtg atgttcggat
     2881 ttgtgcgcca ttatgggctg tctccgtttt tgattcggac aggagaaagc ctaggagcgc
     2941 gtcgacctcg gcacgcatcg agcgcaggat atgcagtgga aagtgcggct ggtcgccgat
     3001 ctcaaaacga gatgctgccg gtttagtcat ccatgctggc aagctggcga atgagccgtc
     3061 gggttgaagt acgacgacga actcgacgcc gccgcattcg agccgccgtc tgatctgtac
     3121 cacttcgcca aagcgcggat ggaatcggta actgatacgg gcttcagctg gctgttcgtg
     3181 ggcagtatgt cgccattccg agaaccgctt ggatcttacg aatatcggta ccgtgctcga
     3241 gcagatgggt cgcgaagctg tggcgcagcg catgcagggt cacgcccttc ctgatcccgg
     3301 ccgcatcggc agcctgatga aacaggcggt tgagctggcg ggtcgacatc gtctggccgc
     3361 gcttggtgcc gggaaacagc caacgttcct gcatcggcgt tccggcatca tgacgcgatg
     3421 ggcgcacctt ccaccattgc cgcaacagat cgagcatctc ggcggacagc atgacattgc
     3481 ggtccttccg gcccttggac tgctcgaccc gaatgatgtt ctgtgcgcgg tcgatgtgct
     3541 tgaccttcag cctgaccacc tcgccggcgc gcagcccgca gccatagccg agactgagca
     3601 gcgtgcgagc cttgagactg ctggccactg ccagcaggcg gcgcgtctcg tcctggctca
     3661 tcaccaacgg aatcttctgc ggctcccgga tgtgatagat ctccgcggca aggtccaacc
     3721 gccgcagcgt cacgcgaagc aggaaccgca gaccggtcat gatgcgattg cgattgcaaa
     3781 tgctcacgcc cgtctcggaa agatgaagct ggaatcggcg gatatcctcc gccgtggccg
     3841 tatcggggga ttgtttgaga aacgcagcga accgcctgca gctgtgaatg tggcccctct
     3901 gcgttcctgc acagagcttg cgtgcgttca tgtcctcgat catgcgctgg cgcagcgggc
     3961 tgaccgtaac cgtgctcata ggcaactcct gtcttgaatg aggttgaaga cgcctcgatc
     4021 ctcaagacag ggcccgacgc ccgctatcga cga
//
LOCUS       DATLJT010001317         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3554053,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001317 DATLJT010000000
VERSION     DATLJT010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3554053"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VM910_30220"
     CDS             complement(<1..188)
                     /locus_tag="VM910_30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66818.1"
                     /translation="MAHHSDSSTHVGQLSSERQSAEKATLIGTLIHLWPYIWPGDRID
                     LKMRVVWSVVLLLVAKLAT"
     gene            390..995
                     /locus_tag="VM910_30225"
     CDS             390..995
                     /locus_tag="VM910_30225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVI66819.1"
                     /translation="MNKIRTVCVYCGSGPGTNPRFIESAKALGKIFAESGIRLVYGGG
                     SLGMMGAVATSVLDHGGIVTGIIPDFLTARENALTRVQEMIVTPDMHERKRLMFERSD
                     AFVALPGGIGTLEELVEQLTWQQLGRHSKPVLLANVDGFWEPLLALLAHMRATQFIRS
                     GLSIDILKAERVEDILPRLRAAAARTPESAKEMAPEIARRL"
     gene            complement(1005..1982)
                     /locus_tag="VM910_30230"
     CDS             complement(1005..1982)
                     /locus_tag="VM910_30230"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66820.1"
                     /translation="MNRRSFMTSLAAAVAWPDISRARSTAKISTIGFLGLAPASAWSS
                     EIEALRAGLRDLGYMEGQNIAIEFRWASSVGDMPALARGLVELNVDVIVAPASTQVAP
                     VRKATSSIPIIFAQHADPVGTGDVASLAHPGGNVTGVSMVLTELAAKGLEILKEAVPS
                     IMRVGVLWNPTAPSHLQVVNQLKIAATSLGLQLIMTPVRETADLSDAFAVLRQEHADG
                     FIVPPSPLTNSQVASLAELELQHRLPGMFGNKDNVRAGGLMSYGADFDYMYRHAATYI
                     GKVLQGEKPADLPVEQASKYYLILNLKTAKALGTELSATVVARADEVIE"
     gene            complement(2030..2407)
                     /locus_tag="VM910_30235"
     CDS             complement(2030..2407)
                     /locus_tag="VM910_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI66821.1"
                     /translation="MIDHVSVGVADLDRAARFYEVTLAALGLSRLVTRPATVGFGKTY
                     PEFWVNLRTDMAKVPHASGMHICLRARTAGEVDAFHAAALSAGGLSDGAPGLRPHDRV
                     KYYAAFVIDPDGNRIEAVTFPQT"
     gene            complement(2494..4092)
                     /gene="cimA"
                     /locus_tag="VM910_30240"
     CDS             complement(2494..4092)
                     /gene="cimA"
                     /locus_tag="VM910_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HVI66822.1"
                     /translation="MSRERLYLFDTTLRDGAQTNGVDFTLTDKQAIAQMLDDLGIDYV
                     EGGYPGANPLDTEFFGKKPKLGHARFTAFGMTRRAGRSASNDPGLAALLEAKADAICF
                     VAKSSAYQVRVALETTNEENLSSIRDSVTAAKKAGREVMLDCEHFFDGYKENPDFALA
                     CAKAAYDSGARWVVLCDTNGGTMPHEVEAIVTEVTKHVPGDHIGIHAHNDTEQAVANS
                     LAAVRAGARQIQGTLNGLGERCGNANLCSLIPTLKLKHEFADTFEIGVSDEQLANLMR
                     VSRTLDDMLNRAPNRHAPYVGESAFVTKTGIHASAVLKDPQTYEHVMPESVGNHRKVL
                     VSDQAGRSNVIAELDRAGITYNKDDPKLARLVEELKEREAVGFAYESANASFELLARR
                     TLGKVPEYFRVEQFDVNVEQRYNANGQRVTVALAVVKVDVAGERLISAAEGNGPVNAL
                     DVALRKDLGKYQKYIEGLKLIDYRVRILNRGTEAVTRVLIESEDEAGESWTTVGVSPN
                     IIDASFQALMDSVVYKLVRSGAPA"
     gene            complement(4089..4589)
                     /locus_tag="VM910_30245"
     CDS             complement(4089..4589)
                     /locus_tag="VM910_30245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI66823.1"
                     /translation="MAQALPKPALRPFLAADAPMLAAIFTASVQELTGDDYSEAQQEA
                     WAAVAEDEQSFGKKLSGELTLIATMQNAPVGFAALKGIDHIDMLYVHPSVAGQGVGAM
                     LCDALEKLAGARGAKNLSVDASDNALDFFKKRGYVAQQRNSVTVNGEWLANTTMRKTL
                     ANGGLA"
     gene            complement(4589..>5245)
                     /locus_tag="VM910_30250"
     CDS             complement(4589..>5245)
                     /locus_tag="VM910_30250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVI66824.1"
                     /translation="HHENEVAQSCCAFHESRMANTWMHNGFLQVEGEKMSKSLGNFFT
                     IRDLLKDWPGEVLRFQMLMTQYRSPIDWTRQSTEQAAVALDSFQHITRTVDAEDGTIS
                     PDVLTALADDLNTSQAIVALHSLAAKARAGDHRSASDLKATCGFLGFTLKDVALQDIL
                     RRQKSDIDANQVNALIAARTAARARKDFKESDRIRDELAAMGIAIKDSKDGTTWEIAR
                     "
BASE COUNT         1000 a         1650 c         1534 g         1061 t
ORIGIN      
        1 gtcgcgagct tggcgaccag aagcagcacc accgaccaca ccacgcgcat cttcaggtcg
       61 atgcggtcgc cgggccagat gtagggccac agatggatca gagtgccgat cagggtggcc
      121 ttttcggcgg attggcgttc cgaggacaat tggccgacgt gcgtagacga atcggagtga
      181 tgagccatca atcgggagcc tgaggaaccg ccggcgcttg tgccgctttt ttcatcgttc
      241 acgagctcat atagagtgtt tggatgtcac gtacaccctt ggtcgtagca attctttcgc
      301 gattaaaggc tatttgcggt tagattgagc cattatgccg aatcattctc cgcttgacgc
      361 cttttacgct gcagtgccac atgcacccga tgaacaagat tagaactgtc tgtgtctatt
      421 gcggctccgg ccccggcacc aatccccgct tcattgagtc agcgaaagca ctcggcaaga
      481 tatttgccga gagcggcatt cgtctggtct atggcggcgg ctcgctcggc atgatgggcg
      541 cggtcgcaac ctccgtgctc gatcacggcg gcatcgtgac cggcatcatt ccggacttcc
      601 ttaccgcacg cgaaaacgcc ctcacccgcg tccaggagat gatcgtcaca cccgacatgc
      661 acgagcgcaa gcggctgatg ttcgagcgct cggatgcctt cgtcgcgcta cccggcggca
      721 tcggcacgct cgaagaactc gtcgagcagc tcacctggca gcagctcggc cggcattcca
      781 agcccgtgtt gcttgccaat gtcgacggct tctgggagcc gcttctggcg ctgctcgcgc
      841 atatgcgtgc cacccaattc atccgctccg gcctgtcgat cgacattctg aaagccgagc
      901 gggtcgagga catcctgccg cggctgcgcg cggccgcagc acgcacgccc gaaagcgcca
      961 aggaaatggc gcccgagatc gcacggcgcc tgtaggccac cgtcctactc gatcacctca
     1021 tccgcgcgtg caacgaccgt cgcgctcagc tcggtgccaa gcgctttcgc ggtcttgagg
     1081 ttgaggatga gatagtattt cgatgcctgc tcgaccggca agtcggcggg cttttccccc
     1141 tgcagcacct tgccgatgta agttgcggcg tgtcgataca tgtaatcgaa atcggcgccg
     1201 tagctcataa gtccgccagc cctgacgttg tctttatttc cgaacatgcc gggcaaacga
     1261 tgctggagtt ccaactcagc caaggaagcg acttgcgaat tcgtgagcgg agacggcggg
     1321 acaatgaagc cgtcggcgtg ctcctgcctc aagaccgcga aagcgtcgct caggtccgcg
     1381 gtctcgcgca ccggagtcat gatgagttgc aagccgagcg aggtggctgc gatcttgagc
     1441 tgattgacga cttggaggtg cgacggtgcc gttggattcc agagaacacc gaccctcata
     1501 attgatggca ccgcctcttt caaaatctcc aagcccttgg ctgcaagctc ggtcagcacc
     1561 atcgaaaccc ctgtcacatt tcctccggga tgcgcgaggc tcgcgacatc ccctgtccca
     1621 acggggtccg cgtgctgggc aaaaatgatc gggatgctgc tggtcgcctt gcgtaccggc
     1681 gcaacctgcg tcgatgccgg tgccacgatc acatcgacgt tcagctcgac cagccccctg
     1741 gcaagcgcgg gcatgtcgcc cacgcttgaa gcccagcgaa actcaatcgc gatattctgt
     1801 ccctccatat atccaagatc gcgcagcccc gcccgcagcg cttcgatttc gctggaccag
     1861 gcggatgctg gagcaaggcc gagaaagccg atcgtgctga ttttcgccgt cgatcgggcg
     1921 cgcgagatgt cgggccaggc gaccgctgcc gcaagagaag tcataaagct ccggcgattc
     1981 attgaaacca cctgctcaca cgtcgtgacc gtgactccca atatcctact caagtctgcg
     2041 ggaaggtcac ggcctcgatg cgattgccgt cgggatcgat gacgaaggct gcataatatt
     2101 tgacgcgatc atggggccgc aagcccggcg cgccgtcgga taacccgccg gcactcagcg
     2161 ccgcagcatg aaacgcatcg acctccccgg cggtcctcgc acgcaggcaa atgtgcatac
     2221 cgcttgcgtg cggcaccttt gccatgtctg tacgcaggtt gacccagaat tccggatagg
     2281 tctttccgaa gccgaccgtt gccggccggg tcaccagacg cgagagccca agcgcggcga
     2341 gcgtcacctc atagaagcgc gccgcgcgat cgagatcggc aacaccgact gacacgtgat
     2401 cgatcacagg tgattacctc caacacaccg tcattgcgag gaacgaatgc gacgaagaaa
     2461 aatttctgga tgcttcgcgc gcaatgaggt gcctcacgcc ggcgcgccgg accttacgag
     2521 cttgtagacc accgaatcca tcagcgcctg gaatgacgca tcgatgatgt tcggcgacac
     2581 gccgaccgtg gtccagcttt cgccggcttc atcctcgctt tcgatcagca cgcgcgtgac
     2641 ggcttcggtg ccgcgattga ggattcgcac gcgataatcg atcagcttca gaccctcgat
     2701 atatttctgg tatttgccaa ggtctttccg cagcgcgacg tcgagcgcat tgaccggacc
     2761 gttaccttcg gccgccgaga tgagccgctc accggcgaca tcgaccttca ccacggccag
     2821 cgccaccgta acgcgttgac cgttggcgtt gtaacgttgc tccacattga cgtcgaactg
     2881 ctcgacccga aaatattccg gcactttccc gagcgtgcgg cgcgccaaca gctcgaacga
     2941 ggcgttggcg gattcatagg cgaagcccac cgcctcgcgc tccttcaatt cctcgaccag
     3001 gcgcgcaagc ttcggatcgt ccttgttgta ggtaatgccg gcgcgatcga gctcggcaat
     3061 cacgttggag cggccggcct ggtcggagac caggaccttg cgatgattgc caaccgactc
     3121 cggcatcacg tgctcgtagg tctgcgggtc cttcagcacg gcggaggcat gaatgccggt
     3181 cttggtgacg aaggcgcttt cgccgacgta aggcgcatgc cggttcggcg cccgattgag
     3241 catgtcgtcg agtgttcgcg acactctcat caggtttgca agctgctcat ccgacacacc
     3301 gatctcgaag gtatcggcaa attcgtgctt caacttgaga gtcgggatca gcgagcacag
     3361 attggcattg ccgcagcgct cgcctaaccc gttcagcgta ccctggatct gtcgtgcgcc
     3421 ggcgcggacg gcggcgagcg aattggcgac cgcctgctcg gtgtcgttat gcgcgtgaat
     3481 accgatgtga tcgccgggga cgtgctttgt cacctcggtg acgatcgcct cgacctcatg
     3541 cggcatggtg ccgccattgg tgtcgcacag cacaacccac cgtgctccgg aatcgtaagc
     3601 ggctttggcg caggccagcg caaaatcagg gttttccttg taaccgtcga aaaaatgctc
     3661 gcaatccagc atcacttcgc gcccggcttt cttcgctgcg gtgacgctgt cgcggatcga
     3721 ggaaaggttt tcttcgttgg tggtttccag cgcgacccgc acctgatagg cggaggattt
     3781 tgcgacaaag cagatcgcgt ccgctttcgc ctccaataac gccgcgagcc ccggatcgtt
     3841 cgacgccgag cgcccggcgc ggcgcgtcat gccgaatgcg gtgaaacgcg catgaccgag
     3901 cttcggcttt ttgccgaaaa attcggtgtc gagcggattg gcaccgggat agccgccctc
     3961 gacatagtcg atgccgagat cgtcaagcat ttgcgcgatc gcctgcttgt cggtcagcgt
     4021 gaagtcgacg ccattggtct gggcaccgtc gcgcagcgtg gtgtcgaaaa gatagaggcg
     4081 ctcgcggctc atgctaaacc tccgttcgca agcgtcttcc gcatggtggt gttggccagc
     4141 cattcgccgt tcacggtcac actgttgcgc tgttgcgcga cataaccgcg ctttttgaaa
     4201 aaatccaaag cgttgtcgct ggcgtcgacc gagagatttt tcgcgccgcg ggcccccgca
     4261 agcttctcca gcgcatcgca cagcatcgcg ccgacgccct gcccggcaac gctcgggtgc
     4321 acatagagca tgtcgatgtg atcgatgcct ttcaacgcgg cgaagccgac cggcgcgttc
     4381 tgcatcgtcg cgatcagcgt gagttcgccg gaaagcttct tgccaaaact ctgctcgtcc
     4441 tcggcaaccg ccgcccaggc ttcctgctgc gcttcgctgt aatcgtcgcc ggtcaattcc
     4501 tggacgctgg ccgtgaagat cgcagccagc atcggcgcat cggccgcaag gaacggccgc
     4561 aacgcaggtt tggggagcgc ctgagccatc atcgcgcgat ctcccaagtg gtgccgtcct
     4621 tggagtcctt gatcgcgatc cccatcgcgg cgagttcgtc gcggatgcga tcggattcct
     4681 tgaaatcttt tcgcgcgcga gccgccgtgc gggcagcgat caaagcgttg acctggttgg
     4741 catcgatatc cgatttttga cgccggagga tgtcctgtaa agccacgtcc ttcagagtga
     4801 agcccaggaa accacatgtc gccttcagat cgctggcgga ccgatggtct cccgcccggg
     4861 cctttgccgc taacgaatga agcgcgacga tcgcttgcga cgtattcaga tcgtcggcaa
     4921 gcgccgtgag cacgtcaggc gagatcgttc cgtcctctgc atccacggta cgggtgatgt
     4981 gctgaaaact atcaagggca actgcagcct gctccgtcga ctgcctcgtc caatcgatcg
     5041 gcgatcgata ctgcgtcatc agcatctgaa accgcagcac ctctcccggc cagtccttca
     5101 gcagatcgcg gatcgtgaaa aaattgccga ggctcttcga catcttctcg ccttcgacct
     5161 gcaggaagcc gttgtgcatc caggtattgg ccatgcggct ctcgtggaac gcgcagcagc
     5221 tctgcgcgac ctcgttctcg tgatg
//
LOCUS       DATLJT010001318         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2636184,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001318 DATLJT010000000
VERSION     DATLJT010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636184"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VM910_30255"
     CDS             <1..1036
                     /locus_tag="VM910_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI66825.1"
                     /translation="SGSAAVRTGWGPRHLIAKLDGAIAGIVPCYLKAHSQGEYVFDRG
                     WADAYERAGGRYYPKLQCSVPFTPATGPRLLMRGDVDRDQIGSALASGLVALCGISKA
                     SSAHVTFAREAESKFLAEHGFLQRTDQQFHWHNQGFATFDEFLATLNSRHRKAIKRER
                     REAVAAGITIHWLTGNDITEDALDAFFAFYMETGSRKWGRPYLTRAFFSLIGESMAKD
                     VLLVMARRNNRWIAGAINFIGSDALFGRNWGAIEHHPFLHFEVCYYQAIDFAIHRHLK
                     VVEAGAQGEHKIARGYLPQTTFSAHYIADPSLRRAIADYLKRERAYVAEAGRELAAAG
                     PFRKVADEAS"
     gene            1085..1513
                     /locus_tag="VM910_30260"
     CDS             1085..1513
                     /locus_tag="VM910_30260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HVI66826.1"
                     /translation="MPAYDPNNAFAKILRGEFPCHKVYEDEHVLAFLDIMPRSPGHTL
                     VIPKAPARNILDIKTEDYLHVARATHKIAVAAKKAFNADGITIQQFNEPAGGQVVFHL
                     HVHVMPRQDGIALLPPASRKEDVKVLADNATKLIAALKGA"
     gene            complement(1583..2068)
                     /gene="tsaA"
                     /locus_tag="VM910_30265"
     CDS             complement(1583..2068)
                     /gene="tsaA"
                     /locus_tag="VM910_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HVI66827.1"
                     /translation="MVRETDIREGEVPVDISPPHDAALYFIGRIRTPWTSRLETPRQG
                     RHDGPICRLEIFEPWVPAIKGVDFYENLEVIYWLHQSRRDLVLQSPKHHGSTRGTFSL
                     RSPVRPNPIATSIVKLVGVEGSTILVRGLDCLDETPLLDIKPDRCEFSPLSAQRADID
                     T"
     gene            complement(2079..2513)
                     /locus_tag="VM910_30270"
     CDS             complement(2079..2513)
                     /locus_tag="VM910_30270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66828.1"
                     /translation="MLPYKGAGPALTDLIAGNIQLMFESVGTALPPVKSGLLRPLGVS
                     ASERITELPDVPTIAESGYPDYRVSVWYGVAAPAKVPDDIAQKITASLDRALNDDAFR
                     ASLEKIGFPPLRPRNAAGIREFIEADRARWARVIKALNISLD"
BASE COUNT          576 a          954 c          899 g          564 t
ORIGIN      
        1 atcggggtcg gcggccgtac gcaccggctg gggcccacgc catctgatcg cgaagctcga
       61 tggtgcgatc gcaggcatcg tgccctgcta tctgaaagcg cattcgcaag gcgaatatgt
      121 cttcgatcgc ggctgggccg atgcctatga gcgcgccggc ggccgttact atccaaaact
      181 gcaatgctcg gttccgttta caccggcgac cggcccgcgc cttctgatgc gcggcgatgt
      241 cgatcgcgac cagatcggat cggcgcttgc gagcggcctt gtggcgctct gtggcatcag
      301 caaggcgtca tccgcccatg tgaccttcgc acgcgaagcg gaatcgaaat ttctcgccga
      361 acacggattt ctgcagcgga ccgaccagca gtttcactgg cacaaccagg gctttgcgac
      421 attcgacgag tttctcgcaa cgctcaattc gcgccaccgc aaagcgatca agcgcgagcg
      481 ccgcgaggcc gttgccgccg gcatcaccat ccactggctg accggcaacg acatcaccga
      541 agacgccctg gatgcgtttt ttgccttcta tatggagacc ggctcacgca aatggggccg
      601 gccctacctg acgcgcgcgt tcttctcgct gatcggcgag agcatggcga aagacgtgct
      661 gctggtgatg gccaggcgca acaaccgctg gatcgccggc gccatcaatt tcatcggctc
      721 cgacgcgttg ttcgggcgca actggggcgc gatcgagcat cacccgttcc tgcatttcga
      781 ggtctgctac taccaggcga tcgacttcgc gatccaccgt cacctcaaag tcgtcgaggc
      841 cggcgcccag ggcgagcaca agatcgcgcg cggctacctg ccgcagacca ctttttccgc
      901 gcattacatc gccgatccca gcctgcgtcg cgccatcgct gattacctga agcgcgagcg
      961 cgcctatgtc gcggaggccg ggcgggagct tgcggccgca ggccctttcc gcaaggtcgc
     1021 ggacgaggcg tcttgaccgc acctgccctt tcgaggaagt tgccgaaaat ttttgggagc
     1081 cgtcatgccc gcctacgacc cgaataacgc cttcgcgaaa atcctgcgcg gcgaatttcc
     1141 gtgccacaag gtctacgagg acgagcacgt gctcgcgttc ctcgacatca tgccgcgatc
     1201 gcccgggcat acgcttgtca ttccgaaagc gcccgcccgc aacatcctcg acatcaagac
     1261 cgaagactat ctgcatgtgg cgcgcgcgac gcacaagatc gcagtcgccg cgaagaaggc
     1321 tttcaacgcc gacggcatca ccatccagca attcaatgaa cccgccggcg gacaggtcgt
     1381 gtttcacctg catgtccacg tgatgccgcg ccaggacggc attgcgctgc tgccgccggc
     1441 aagccgcaag gaagacgtca aggtcctcgc agacaacgcg acgaaattga tcgcggcgct
     1501 gaagggcgcc taaccgcgtc ctgcatcgtt ccgggcatga cgagtgcaag ccggagatcc
     1561 atcgccaaga gtacgatacg actcacgtat caatatcggc ccgttgcgcc gaaagcggcg
     1621 agaattcgca acgatcaggc ttgatgtcca gcagcggcgt ttcgtcgagg caatcgagcc
     1681 cgcgcaccag gatcgtgctt ccttcgacgc caaccagttt cacgatcgac gtggcaatcg
     1741 gattgggccg caccggcgag cggagcgaaa aggtaccgcg cgtcgatccg tggtgcttcg
     1801 gactctgcag cacgaggtcg cggcgcgact ggtgcaacca gtagatgacc tcgagatttt
     1861 cgtagaagtc gacgcccttg atcgccggca cccacggctc gaaaatctcg agccggcaga
     1921 tcggcccgtc atggcggccc tgccgcggtg tctccaaccg cgaggtccac ggggttcgga
     1981 tccgcccaat gaagtaaagc gcggcatcgt gcggcggcga gatgtcgacg ggcacttcgc
     2041 cctcgcggat gtccgtttct ctaaccatcg tgtcttctct agtccaaaga tatgttgagc
     2101 gccttgatca cgcgcgccca ccgcgcgcga tcggcctcga tgaattccct gattccagcg
     2161 gcattgcgtg gacgaagcgg cggaaagccg attttctcga gggaagcgcg gaaggcatcg
     2221 tcgttcaggg cccgatcgag gcttgcggtt atcttttgcg cgatatcgtc cggcacctta
     2281 gcgggtgccg caacaccata ccagacgctg acgcggtaat ccggataacc gctctcggca
     2341 atggtcggaa catcgggcag ctccgtgatg cgttcgcttg cgctgacgcc gagcggacgc
     2401 agcagcccgg acttgaccgg cggcaacgcg gtgccgaccg attcgaacat caactggatg
     2461 ttacctgcga tcaggtcggt cagtgcgggc cccgcgccct tgtagggcag cagagatgca
     2521 caaaaaacgc tgcaatatca aaatgaatcg agatcggggt cgggtcgagg tcgggagaag
     2581 aaattgcact gatctgtcaa cgcatcgttc gcgcatcatg cacgtcgttc agccgcgcta
     2641 cgactcccac gcgcgatggc gtcctgtgcc actgctccaa cctgtgcgat ggactgatgt
     2701 ccgtggcccc gcatttcgat gaacctgatt gcggccggtg cgcgatatat ttcgctccgt
     2761 cgaagtcaac gcccatgggc ttgtgcgcgc tctcttgcgg ctttgatttc cggtttctct
     2821 ggtatcactt cgaatctccc tcgtctgacc ccgccaatag gccggcgaat ttggatggcg
     2881 gttgctggtg gatgccctgc gcgatccggg tgcgggcaat cggcgtcaga ccaaactcgg
     2941 ccgcaaacct gatcatgtcg gcggcagcgt cagcggcgat cttgatcaaa gga
//
LOCUS       DATLJT010001319         5996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_106407,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001319 DATLJT010000000
VERSION     DATLJT010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106407"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VM910_30275"
     CDS             complement(<1..327)
                     /locus_tag="VM910_30275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66829.1"
                     /translation="MFSIRCTRWTVAALALVATFAPAAISAQTPEHPNDNTAQFPTRN
                     DLKSLTMSGSYLAARHAGVERDATSAAAFYRSALRTDPKNNELLDRAFISSVADGDID
                     EAVKLGE"
     gene            complement(578..2236)
                     /locus_tag="VM910_30280"
     CDS             complement(578..2236)
                     /locus_tag="VM910_30280"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HVI66830.1"
                     /translation="MSSEELPPRESMEFDVVIVGAGPSGLSAAIRLKQLNADLNVVVV
                     EKGSEVGAHILSGAVIDPAGLDKLVPDWREDADCPLKTEVKDDRFYWMSGGSAIRLPN
                     FMMPPLMNNHHCYIGSLGQVCRWLARKAEALGVEIYPGFAAVEVLYDDKGAVRGIATG
                     DMGIAKDGTHKSSFTRGMELVGKYTLFAEGARGSLTKQLVNKFSLAANSEPPKFGIGL
                     KEVWQIDPAKHQKGLIQHSFGWPLNNKTGGGSFLYHYDDNLVAVGFVVHLNYDDPYLS
                     PFDEFQRFKTHPAIRPIFEDAKRLAYGARAITEGGYQSVPRLSFAGGALIGCAAGFVN
                     VPRIKGVHNAMGSGMLAAESVAAALAAGRANDELVEYENAWRGSAVGKDLYRVRNVKP
                     LWSKFGTVLGVGLGGLDMWCNTLGFSLFGTQSHAKPDRATLDPAKTHTPIAYPKPDGK
                     ISFDKLSSVFLSNTNHEEDQPVHLRVADMNLQKTSEHDVFAGPSNRYCPAGVYEWVEE
                     AAGPRFQINAQNCVHCKTCDVKDPNGNITWVPPEGGGGPNYEAM"
     gene            2357..3172
                     /locus_tag="VM910_30285"
     CDS             2357..3172
                     /locus_tag="VM910_30285"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVI66831.1"
                     /translation="MSQALPEPAPNVAQLLAFYLEAGIDCALDEAPVNRLAEHEPPAP
                     REVTPPQPPRAMPAAAIAVARADAPPAPEAAIASAREAARTAPSLEALRELMQNFDGC
                     ALKSTATRLVFADGNPNARIMFVGEAPGRDEDLEGLPFVGRSGKLLDRMIAAIGLDRS
                     NVYIANVIPWRPPGNRTPTPQETQICLPFIQRQIELVDPDLLVTLGNPSTQTLLGTRE
                     GIMRTRGRWFDYDTGTRVIRALPTFHPAYLLRSPSYKRLSWQDLRVIAKALTA"
     gene            complement(3177..3572)
                     /locus_tag="VM910_30290"
     CDS             complement(3177..3572)
                     /locus_tag="VM910_30290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI66832.1"
                     /translation="MAAFRGSCLCGEVAFEIEGPFDRFLNCHCSRCRKATGSAHSCEV
                     IVKAPALCWLKGEGSVARFDLPQAKSFATAFCKRCGSPMPHLTRSGREAIVHAGAFDE
                     PLGATPDRHAQGSSRADWYVHGEGLAVEE"
     gene            complement(3669..5177)
                     /locus_tag="VM910_30295"
     CDS             complement(3669..5177)
                     /locus_tag="VM910_30295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVI66833.1"
                     /translation="MRFTSLVIELIRARPRLVVWIVVLLQAALWLAVSLLLYASPPGD
                     LATALAFGREYQVGSDLGPPLAFWLADIAFRIAGNHMFGVYLLAQLCSVATLWILYQL
                     ARAIVGKQQAVLVVLLTMTITAFASPNLEFGPLVAARPLWALLLFHSWQLIGQNRRNV
                     WFAWSIEAGLLLLTTSAAFGLLLLIAGFALASPEGRRTLRSFDPLFALLVIMVLALPY
                     FVWLMRTDVSLLPPLPALSELGARALRWVSLVGALLLAMAGVLILIVLNSRLFDRAAD
                     ESPIIYRPPVDPLARDFVYFFAIAPALAGSLISGLFNLDRVTGGSGITLLMSGLGAIV
                     ATGDLVHLRRQRLLRSVWAVIIIVPALVAVAAILFLPWTSGREVATSLPARSIAQFFG
                     DSFERRTNQRLRAVAGDAQIASLVALARGRPHLFLDATPQRTPWVTPQKFSETGGVVV
                     WRASDTSGTPPPEIAQRFPGLVPEVPRAFEWLVNGRQPLLRIGWAIVRPKGQ"
     gene            complement(5333..>5996)
                     /locus_tag="VM910_30300"
     CDS             complement(5333..>5996)
                     /locus_tag="VM910_30300"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVI66834.1"
                     /translation="IKRGVWPVAGCDEAGRGPLAGPVVAAAVVLDPKRIPKGIDDSKR
                     LTPEEREDLFEKICATSSFAVAFASPARIDRDNILRASLWALARAVHALPEAPQHVFV
                     DGRDRLALPCECQAVIGGDGLVMSIAAASIVAKVTRDRLMSALAQDCPGYGFEQHKGY
                     AVPEHLEALARLGPSAHHRRLFAPVVAAREKHFGPDQAVLDLDIEALTLDEAAEGLAT
                     NG"
BASE COUNT         1152 a         1947 c         1896 g         1001 t
ORIGIN      
        1 ctcgccgagc ttgaccgcct cgtcgatgtc gccatcggcg accgaggaga tgaaggcgcg
       61 atcgagcagt tcgttgttct tcggatccgt gcgcagcgcc gaacggtaga acgcggcggc
      121 cgaggtcgca tcccgctcca ctccggcatg gcgggcggcg agatagctgc ccgacatggt
      181 cagcgatttc agatcgttgc gggtgggaaa ttgcgccgtg ttgtcgttgg gatgctcggg
      241 cgtctgagcc gaaatcgcgg ccggcgcgaa cgtcgcgaca agagcaagag cagcgacggt
      301 ccagcgagtg caacgaatag aaaacatcac ggttcgctct gcctttttga ggaatttgcg
      361 ggaacgtctc gaatgcaaca gcggcatgca agatgcgcga caatgccgcg tttggcgcct
      421 aaccgcaagg ataagcggtg acaattcgta tcgctcagcc gacagagtat ccccttcggc
      481 cggtcccgga ctaccctcaa aaccgcttca ctgtggccgt atcgtggcca ttcaatgaac
      541 ccaccgattt cgcggactca cgcaatcgtg ctttgcgtca catcgcctcg tagttcggcc
      601 cgccgccgcc ctcgggcgga acccacgtga tgttgccgtt cgggtctttc acatcgcagg
      661 ttttgcagtg gacgcagttc tgggcgttga tctggaatcg ggggccagcg gcctcctcca
      721 cccattcata gacaccggcc gggcaataac ggttcgacgg accggcaaag acatcatgct
      781 cggaggtctt ctgcagattc atgtcggcga ccctgagatg aaccggctgg tcctcttcgt
      841 gattggtgtt ggacaggaac accgacgaca gcttgtcgaa cgaaatcttg ccgtcgggtt
      901 tcgggtaggc gatcggcgtg tgcgtcttgg ccggatcgag cgtcgcgcgg tcgggcttcg
      961 catgcgactg ggttccgaac agcgaaaagc cgagcgtgtt gcaccacatg tcgagaccgc
     1021 cgagccccac gcccaaaacc gttccgaact tcgaccacag cggcttgacg ttgcgaaccc
     1081 tgtagaggtc cttgccgaca gcggagccgc gccaggcatt ttcatattcg acgagttcgt
     1141 cattggcgcg cccggccgca agcgcggctg cgacactttc tgcagcgagc atgccgctgc
     1201 ccatcgcatt gtgcacgccc ttgatgcgcg gcacgttgac gaagcccgcg gcgcagccga
     1261 tcagcgcgcc gccggcaaag gacagacgcg gcaccgactg atagccgccc tcggtgatcg
     1321 cgcgcgcgcc gtaggcgagc cgtttggcgt cctcgaagat cgggcgaatc gcgggatggg
     1381 tcttgaagcg ctgaaattcg tcgaacggcg acaggtaagg atcgtcatag ttgagatgca
     1441 cgacgaagcc gaccgcaacc agattgtcgt cataatgata aaggaaggag ccgccgccgg
     1501 tcttgttgtt gagcggccag ccgaaggagt gctggatcag ccccttctgg tgttttgcag
     1561 gatcgatctg ccagacttcc ttcaggccga tgccgaattt cggcggctcg ctgttggcgg
     1621 cgagcgaaaa tttgttgacg agctgcttcg tgaggctgcc gcgcgcgcct tcggcaaaca
     1681 gcgtgtattt gccgacgagt tccatgccgc gcgtgaacga ggacttgtgg gtgccgtcct
     1741 ttgcgattcc catgtcgccg gtggcgatgc cgcgcaccgc gcccttatca tcgtagagca
     1801 cttccaccgc ggcaaagccg gggtagatct cgaccccgag cgcctcggcc ttgcgtgcaa
     1861 gccaccggca cacctggccg agggagccga tgtagcaatg atgattgttc atcagcggcg
     1921 gcatcatgaa gttcggcagc cggatcgcgc taccgccgct catccagtag aagcgatcgt
     1981 ccttgacctc cgtcttcagc gggcagtcgg cgtcctcgcg ccagtccggc acgagcttgt
     2041 cgaggccagc cggatcgatc accgcacccg acaggatgtg cgcgccgacc tcggagccct
     2101 tttcgacgac gacgacgttg aggtcggcat tgagctgttt cagcctgatc gctgcggaaa
     2161 ggcccgaggg acctgcgcca acgatgacga cgtcgaattc catggattcg cgcgggggta
     2221 attcttcgct gctcatgtcc agatccccga gtgctgttct gatggcgttt ccagttgtcc
     2281 ttgtttccga tttttgctga gaggacaacc gtggaaatgc atttcaagct gccgcgacgc
     2341 ggcgtaaatt gacgtgatgt cgcaggccct gcccgaaccc gcccccaatg tcgcgcaatt
     2401 actggccttc tacctggagg ccggcatcga ctgcgcactc gacgaggcgc cggtcaaccg
     2461 gctggccgag cacgaaccgc cagccccgcg cgaagtaacc ccgccgcagc ccccaagggc
     2521 gatgccggcc gccgccatcg cggttgctcg cgccgatgcg ccgccggcgc cggaggcagc
     2581 gatcgcgtcg gcaagggaag ccgcgcgcac ggctccaagc ctggaggcgc tgcgtgaact
     2641 gatgcaaaac ttcgacggct gcgcgctgaa atcgacagca acccggctcg tgttcgccga
     2701 cggaaacccg aacgcgcgca tcatgttcgt cggcgaagct cccgggcgcg atgaagacct
     2761 tgagggcctg ccgttcgtcg gccgttcggg caagctgctc gaccgtatga tcgccgcaat
     2821 cgggctcgat cgcagcaatg tctatatcgc caatgtcatc ccctggcgcc cgcccggcaa
     2881 tcgcacgccg accccgcagg agacgcagat ttgcctgccc ttcattcagc gccagatcga
     2941 actggtcgac cccgacctgc tggtaacgct cggcaatccc tcgacccaga cgctgctcgg
     3001 cacccgcgag ggcatcatga gaacgcgcgg ccggtggttc gactatgata ccggcacccg
     3061 cgtgatccgc gccctgccga cgttccaccc cgcttacctg ttgcgctccc cgtcctacaa
     3121 gcgattgtcc tggcaggatt tgcgggtgat cgcgaaagcg ctgacggcgt agcggttcat
     3181 tcctccaccg ccaatccctc gccatggacg taccaatcgg cacgcgagga cccttgagcg
     3241 tgccggtcgg gcgtggcgcc aagcggttcg tcaaatgctc cggcgtgaac gatggcctcc
     3301 cgcccgctgc gagtgagatg cggcatggga ctgccgcacc tcttgcagaa cgccgtagca
     3361 aagcttttcg cctgcggcag gtcgaagcgt gcgaccgatc cttctccctt gagccagcag
     3421 agcgccgggg ctttgacaat tacttcgcag gagtgcgcgc tcccggtcgc tttgcggcag
     3481 cgtgagcaat gacagttcag gaaccggtcg aatggtccct cgatctcaaa tgcgacttcg
     3541 ccgcacagac agcttcctcg aaatgccgcc atcctcgact cctggtctgc tgcaggcctt
     3601 gcacaatcct gacgatgtcg ctcgtgcaat gattgagtcg gcccgacgtt gcaagcccag
     3661 gggtgtgttc actgcccctt cggccgcacg atggcccagc cgatccgtag caagggctgg
     3721 cggccattca ccagccattc gaacgcccga ggcacctcgg gcacgagccc cggaaagcgc
     3781 tgcgcgatct cgggcggcgg cgtgcctgac gtgtcggagg ctcgccagac cacgacgccg
     3841 ccggtctcgc tgaatttctg cggtgtcacc cacggggtgc gttgcggggt ggcgtcgaga
     3901 aacagatgcg gccgtcccct ggcgagcgcg accaggctcg caatctgcgc atcgcccgca
     3961 accgcacgca gccgctgatt ggtacggcgc tcaaagctgt cgccgaaaaa ttgcgcgatc
     4021 gaacgcgctg gcagcgatgt cgcgacctcg cgaccgctgg tccagggcag aaacagtatg
     4081 gccgcgacgg ccacgagggc gggcacgatg atgatgactg cccatacaga acgcaacagg
     4141 cgctgccggc gcaagtggac gagatccccg gtcgcgacga tcgccccaag gccggacatc
     4201 aagagcgtga tcccggaccc tcctgttacg cgatcgagat tgaaaagccc ggagatcagg
     4261 cttccggcaa gcgcgggcgc gatggcgaaa aaatagacga aatcgcgcgc aagcggatcg
     4321 accggcggcc gatagatgat cggagattcg tccgccgcgc gatcaaacag ccgcgagttg
     4381 agcacgatca ggatcaggac gcccgccatc gcgagcagca gcgcaccaac cagcgacacc
     4441 caccgcaagg cccttgcgcc cagttccgaa agcgccggga gcggcggcag cagggatacg
     4501 tcggtgcgca tcagccagac aaagtacggc agtgccagca ccatgatcac gagcagcgcg
     4561 aacagcggat cgaacgatct gagcgtgcgg cggccttccg gactggccag cgcaaagccc
     4621 gcgatcagga gcaacaggcc gaaggcggcc gacgtggtca gcagcaagag accggcttcg
     4681 atagaccacg caaaccagac attgcgccgg ttctggccga tgagctgcca ggagtggaac
     4741 aacaacagcg cccacagcgg tcgcgccgcg accaggggac cgaattcgag gtttggcgag
     4801 gcgaacgcgg tgatggtcat ggtcaacagc acgacgagga ccgcctgctg cttgccgacg
     4861 atcgcgcgcg caagctggta gaggatccac aaggtcgcaa cagagcaaag ctgcgcgagc
     4921 agatagacgc cgaacatgtg attgccggcg atgcggaagg cgatgtcggc gagccagaac
     4981 gccagcggcg ggccgagatc gctgccgacc tgatactccc ggccgaaggc gagcgcggtg
     5041 gcgagatcgc ctggagggct tgcgtataac aacagcgaca ccgcgagcca gagcgcagcc
     5101 tgcagcaaca ccacgatcca gaccaccagc cgcggccgcg cccggatcag ttcgatcaca
     5161 agtgaggtga aacgcatgga acttccgaag atccccagac cagccacgcc ggtcgtgcgt
     5221 tggcccgccc ggacgttttg ataaagacgg gacacgatcg aggcaacctg ttgtcccgtc
     5281 tcaacacatc agtcgcgaca cttccgcttg cgatcggcac gagcgcccac gatcagccgt
     5341 tagtagcaag gccctcggcc gcctcgtcca gggtcagcgc ttcaatatcg aggtcgagca
     5401 ccgcctggtc cggcccaaaa tgcttttccc gtgcggcgac gacgggagca aaaagtcggc
     5461 ggtgatgcgc ggacggtccg agccgggcga gcgcttcgag atgctccggg accgcgtagc
     5521 ccttgtgttg ctcgaagccg tagcccggac aatcctgcgc cagcgcgctc atcaggcgat
     5581 cgcgcgtcac ctttgcgacg atcgaggctg ccgcgatcga catcacgagc ccgtcgccgc
     5641 cgatcaccgc ctggcactcg cagggcagcg cgaggcggtc gcggccatcg acgaacacgt
     5701 gttgcggagc ttccggcaac gcatgcaccg cgcgggccag cgcccagagc gaggcccgca
     5761 ggatattgtc gcgatcgatc cgcgcgggcg aagcaaaggc gaccgcaaag gatgaggtcg
     5821 cgcatatctt ttcgaacagg tcctcgcgct cctcgggcgt cagccgcttc gaatcgtcga
     5881 tgccctttgg aatccgcttg ggatcgagca cgacggcggc cgcgacgacc ggtcccgcca
     5941 atggcccgcg cccggcctcg tcacagccgg ccaccggcca gacgccccgc ttgatc
//
LOCUS       DATLJT010001320         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1257629,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001320 DATLJT010000000
VERSION     DATLJT010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257629"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VM910_30305"
     CDS             <1..369
                     /locus_tag="VM910_30305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI66835.1"
                     /translation="LSVSPCHRRVKLLEERGVITRYIATVDQKSVGLHVSVFISIKLA
                     RQKEEDLDRFARAISKWEEVLECYLMTGNRDYLLRVVAADLSSYEAFLKNKLTRLDGI
                     ASIESSFALSQVKYSIALPV"
     gene            542..928
                     /locus_tag="VM910_30310"
     CDS             542..928
                     /locus_tag="VM910_30310"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI66836.1"
                     /translation="MTDTLLLIGRVLLAVVFLMTVWTGGPNADYLKSLNYPSPAVMSD
                     IAHVAEWLIVISLVLGIGTRYGALLALVFVVIAFGTAHRYWQFPQAAQNLQFILLSKD
                     LAIAGGLLVLFVTGGGRFSIDEYLKR"
     gene            1181..2002
                     /locus_tag="VM910_30315"
     CDS             1181..2002
                     /locus_tag="VM910_30315"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI66837.1"
                     /translation="MTPNPNKALWEKGDFTRLAANMRESGEALVSALGIEKGLKVLDL
                     GCGDGTTAIPQAKRGADVLGVDIASNLVAAGNRRVKELGLTNCRFQEGDASNLHELEN
                     DSFDLVVSIFGAMFAPKPFDVAKEMVRVTRPGGRIVMGNWIPGDPTLVAQVLKISSAY
                     TPPPPEGFISPMTWGVESNVIERFGAAGIPKEQVSFLRDNYTFNFPGSPREFVGVFRD
                     YYGPTMNAFDAASKNDRAGDLQKEMEALFERENKSQSKDKTSITATFLRVTVSVT"
     gene            complement(2031..>2363)
                     /locus_tag="VM910_30320"
     CDS             complement(2031..>2363)
                     /locus_tag="VM910_30320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66838.1"
                     /translation="ATTPTTEAPAKECRNVPPLMLYVATTNGGGTVRFREGSYLSPPI
                     TLSTEPQPVIFPRARSQTTTIEELVTIEGQATDLVTISPLSGARHEYPVINGSLALTA
                     TWLPFKGC"
BASE COUNT          473 a          707 c          720 g          463 t
ORIGIN      
        1 ctctcggtct cgccctgcca ccgccgcgtc aagctcctgg aagagcgcgg cgtgatcacg
       61 cgctacatcg caacggtcga ccagaagtcg gtcggcctgc atgtctcggt cttcatctcg
      121 atcaagctcg cgcggcaaaa ggaggaagac ctcgaccgtt ttgcgcgagc gatctcgaaa
      181 tgggaggagg ttctggagtg ctatctgatg acaggcaacc gcgactatct gttgcgcgtg
      241 gtcgcggccg acctttcctc ctatgaggcg ttcttgaaga acaagctcac gcggctcgac
      301 ggcatcgctt cgatcgagag cagttttgcg ctgagccagg tgaaatactc catcgcattg
      361 ccggtctagc gcggcgggac gaacgcaaga tcagtgtgga gcgcggtccg gaaatcggct
      421 gcctcccttt cccgaggccg aagccagcgt taggatttgc gacagcaatc cagggagggc
      481 ggctatgaca gatccatcat cacatccgta tctttcgtat gcagacggtg tcgcagtcgg
      541 aatgaccgac acattgcttc tcatcggacg cgtgctgctc gccgtcgtct tcctgatgac
      601 ggtatggacc ggaggaccaa acgccgacta tctgaaatcc ctcaattatc cctcccctgc
      661 cgtcatgagc gacatcgcgc atgtggccga atggctcatc gtcatttcgc tggttctcgg
      721 tatcggcacg cgttacgggg cgctgcttgc gctggttttc gtggtgatcg ccttcggcac
      781 ggcgcatcga tactggcaat ttccgcaagc cgcgcaaaac ctgcaattca ttctcctcag
      841 caaggatctt gccattgccg gtggtcttct cgtgctcttc gtgacaggtg gcggacgctt
      901 cagcatcgac gaatacctca aacgttgatg tgccccggca gtgcggccgc acccgcgcga
      961 ccatcagatc ggccgagctg cgtgaccaaa tgtcgcgacg acaaaggggc ggcttgatga
     1021 tcgcttccgt gtgctcaaag ctgcaggagc agaaggcatg tcagggagag tgaccccgcc
     1081 ataaatcgga tcgcgccaac tggcgatcgc aggcgtgcca ttcagcgctg cctttgccgc
     1141 aacgcgcgct ttcaacagtc aatattgcga ggagatttcg atgaccccga acccgaacaa
     1201 ggcactttgg gaaaaaggcg acttcacgcg acttgcagcg aatatgaggg agagcggcga
     1261 agctctcgtg agcgctctcg ggatcgaaaa agggctcaag gtgctcgacc tcggatgcgg
     1321 cgacggcacg accgcgatcc cgcaggccaa gcgtggcgcc gacgtgctgg gcgtcgatat
     1381 cgcaagcaac ctggttgccg cgggcaaccg gcgcgtcaag gaactgggcc tcaccaattg
     1441 caggtttcag gaaggcgacg cctcgaactt gcacgaactc gagaatgaca gcttcgatct
     1501 cgttgtcagc atctttggcg ccatgttcgc gccaaagcct ttcgacgtcg ccaaggagat
     1561 ggtgcgcgtc acgcgccccg gcggacggat cgtcatgggc aactggattc ccggcgatcc
     1621 gacgctggtc gcgcaggtct tgaagatcag ctcggcctac acgccgcctc caccggaagg
     1681 gttcatcagc ccgatgacgt ggggcgtcga gagcaatgtg atcgagcgat ttggggccgc
     1741 gggaattccg aaggagcagg tctcgttcct gcgcgacaat tacactttca actttcccgg
     1801 ctccccgcgg gaattcgtgg gggtgttccg cgactattac gggccgacca tgaatgcttt
     1861 cgacgcggcg tcaaagaacg accgggccgg cgacctgcag aaggagatgg aagctctctt
     1921 cgagcgagag aacaagagcc agagcaagga caagacgtcc atcaccgcga cctttttgcg
     1981 ggtgaccgtt tccgtcacct gatgcagcca acggccgagg tgaacgctct ctagcagccc
     2041 ttgaaaggca accacgttgc cgtgagcgcc aaggatccat tgatgaccgg atactcgtgc
     2101 cgcgcaccgc tcaacggcga aatagtcacg agatcggtcg cctgcccttc gatcgtcacg
     2161 agttcctcga tcgtcgtggt ttgcgaacgc gcgcgcggaa atatcacggg ctgcggttcg
     2221 gtcgaaagcg tgatcggagg cgaaaggtaa ctgccttcgc ggaagcgaac cgtgccgccg
     2281 ccgtttgtgg ttgcgacata cagcatcagc ggcggcacgt tcctgcactc tttcgccggc
     2341 gcctcggtgg tcggcgtcgt cgc
//
LOCUS       DATLJT010001321         1515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2405328,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001321 DATLJT010000000
VERSION     DATLJT010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405328"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VM910_30325"
     CDS             complement(<1..539)
                     /locus_tag="VM910_30325"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66839.1"
                     /translation="MPRKNGKLAISRRTVLMGGAALGVLPSRRAFSQASEPLKVGFLT
                     VNTGPLAAGGKQQVEGAAFYLKERGGMIAGRKVELITEDTAGNPALARTKTLELVERY
                     KVPVMIGPLATNEALAIDSYIRDTKVPLITTTSAATVDLKTHAVNPWVLHAFGTAPQV
                     TYPLGDYAAKTLGFKRMAIV"
     gene            complement(798..992)
                     /locus_tag="VM910_30330"
     CDS             complement(798..992)
                     /locus_tag="VM910_30330"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HVI66840.1"
                     /translation="MNDETKEVGLWDRTKAWADKNRTISGALVGGAAGTVVPGLGTLI
                     GAIVGAGVGFASSKEKKEDR"
     gene            complement(1248..>1515)
                     /locus_tag="VM910_30335"
     CDS             complement(1248..>1515)
                     /locus_tag="VM910_30335"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66841.1"
                     /translation="PTTIEAGYKNSDYTFWTGLFVPAKTPREIVDKLAQETQQALAAP
                     GVREKLAQRGMDPMPITPAAFDAQIRREIHSILALAKAANLKFN"
BASE COUNT          258 a          493 c          457 g          307 t
ORIGIN      
        1 acgatcgcca ttcgtttaaa gccaagcgtc ttggccgcgt aatcgcccag tgggtaggtc
       61 acctgcggcg ccgtgccgaa ggcgtgcagc acccacgggt tcaccgcgtg agtcttcaga
      121 tcgacggttg ctgcagaggt cgtggtgatg agcggcactt tggtatcgcg aatatagctg
      181 tcgatcgcca aggcctcgtt ggtcgccaac ggaccgatca tcaccggcac cttgtagcgt
      241 tcgaccagct ccagggtctt ggtcctcgcg agggcgggat tgccggccgt gtcctcggtg
      301 atcagctcga ccttgcggcc cgcgatcatg ccgccgcgtt ccttgagata aaaagccgca
      361 ccctccacct gttgcttgcc gccggcggcc agcgggccgg tattgacggt gaggaagcca
      421 actttgagcg gctccgaggc ctgcgaaaat gcgcgccgcg atggcagtac gccgagtgct
      481 gctccaccca tcagcaccgt gcggcgcgag atcgcaagtt taccgttctt acgtggcatc
      541 ttcgaactcc gtgaaatttt tccgactttc ctgccaagcg cagacgcaat gagatgacga
      601 gccgattccc gcgccaacgt gcgcagtcgt gtcgaattta cggaaagtgt taaccgggct
      661 ttctcacccc gtaagtatgc gcggggcacg tatccccacg cgcgagaccc cccgggcctg
      721 tccacgatgg gcgcagggcg cgctgcgacg gcagcgcagt tgtggccctg cgttttcgcg
      781 gatcagtcta gccgccgcta acggtcttct ttcttttcct tcgatgaggc aaatccaacc
      841 cccgcgccga cgatcgcgcc gatcagggtg ccgagccccg gcacgaccgt cccggccgcg
      901 ccgccaacga gcgcgccaga aattgtgcgg ttcttatcgg cccaagcttt cgtccgatcc
      961 cacaggccaa cttctttggt ttcgtcgttc atgttgcttc cctcactaaa agagcagctg
     1021 ggatgttagc ctatccgccg catcggcgcg aggtcgacca cattcgcgtc cggacgggcg
     1081 agaccccagc tgtcccatgg tactgcgagc ctcgtcaatg gaaagttcaa cgtcttacag
     1141 gccaacaaaa tttccgttca cgcgcgagcc ccccgtcctg tccagggtgg ggtgcgagcg
     1201 cgctgcccga ggcggcgcgt cctgcccata gtcacgacca atgccggtca gttgaacttc
     1261 aggttcgccg ctttggccaa ggcgagaatt gaatggattt cccgcctgat ctgcgcatcg
     1321 aaggcagcgg gcgtgatcgg catcgggtcc atgccccgct gcgcgagttt ctcgcgcacg
     1381 cccggcgcgg ctaacgcctg ctgcgtctcc tgggcaagct tgtcgacgat ctcgcgcggt
     1441 gtcttggccg gcacgaacag cccggtccag aacgtgtagt cggaattctt gtagccggcc
     1501 tcgatcgtgg tcggc
//
LOCUS       DATLJT010001322         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1026257,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001322 DATLJT010000000
VERSION     DATLJT010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026257"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1551)
                     /locus_tag="VM910_30340"
     CDS             complement(241..1551)
                     /locus_tag="VM910_30340"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVI66842.1"
                     /translation="MDRRGFIRSVAMAGAGAALAVSHRVACAEAEVAATTKRFSQQRW
                     ALDNIIRANGIDWDQPRSIYLSAPCGVEANADFAAIRARVQKMADIGPAFESTAKRRE
                     AKARAAEADGNPVTARDNWYMAAIHYGAAEWPYDDSGPRHVELHNKKRECFSNYARLA
                     DHKIEAVSIPFKTKSIPGWLHLPPGYSGGKVPVVIAIPGMDSFKEVNVALANDRWMQR
                     GVAVLSIDGPGQYESPLLGVYVSMQNWIDAGPAVVDWLLDRPEIDGQKIGVSGASFGS
                     FFGTILTANEPRIAATAVISTCLEPGCHTIFEEASPTFKKRFMWMSNYTDEAKFDEFA
                     KTLTWEGHVEKIRTPYLVVAGEADELSPLEHTERMVRAMSGPRQLVIYADSRHSVGNV
                     PAANLGPFPPALVADWLVARLNGKPVNSERWFVDPTGRIIKTAL"
     gene            1838..>2181
                     /locus_tag="VM910_30345"
     CDS             1838..>2181
                     /locus_tag="VM910_30345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI66843.1"
                     /translation="MRMIDLSRELYHRTPTYPGQPPIIHGVWKTHEESFVESGNVHGN
                     CVMFFSMPDHGGTHIDAPRHFGQNGTPIDEYPLEYCYVKGICLDLRHMAPKAEITPSH
                     LEEAVRKSGAAIP"
BASE COUNT          411 a          675 c          692 g          403 t
ORIGIN      
        1 gggcgggcag ggcggttgcg gcgaagagcc cgcataggcc aagcgtcgcc cccacgaggc
       61 aaagcgtaag acggagcatg accgtatccc cagcggcaca atgttgttgt gacagcaatc
      121 atgcgggcca gagcaaatgc caacaaggcg gcgcagcatg cagatgcctc atgggtgacc
      181 tgcgccagca tacgtgccgc tgcacctgcg ggacggcgcg cggcgatacg cccgacgatc
      241 tcaaagggcg gttttgatga tacggccggt ggggtcgacg aaccagcgtt cgctgttgac
      301 gggcttgccg ttgagccgcg cgacgagcca atcagcgacc agcgccggcg ggaaggggcc
      361 gagattggct gcgggcacgt tgcccaccga gtgtcggctg tcggcataaa tgaccagttg
      421 gcgcgggcct gacatggcgc gaaccattcg ctcggtgtgc tcgagaggac tgagctcatc
      481 ggcttcgccc gcgaccacga gataaggcgt tcggatcttc tcgacgtggc cttcccaggt
      541 cagggtcttc gcgaactcat caaacttcgc ctcgtcggtg tagttcgaca tccacatgaa
      601 acgcttcttg aacgttggcg atgcttcctc gaagatcgtg tggcatccgg gctcgaggca
      661 ggtggaaatc accgcggttg cggcaattcg cggctcgttg gcggtcagga tcgtgccgaa
      721 aaacgagccg aagctcgctc cggaaactcc gatcttctgg ccatcgattt ccggacgatc
      781 caacagccag tccacgaccg cgggtccggc atcgatccag ttctgcatcg agacataaac
      841 gccgagcagc ggactttcgt attgccctgg tccgtcgatc gacaagaccg cgacgccgcg
      901 ctgcatccag cggtcgttcg ccagcgccac attgacttcc ttgaagctgt ccataccggg
      961 gatggcgatc accactggca ccttgccgcc gctatagccg ggcggcaggt gaagccagcc
     1021 cggaatcgat ttggtcttga atgggatgga aaccgcttcg atcttgtggt cggcgagccg
     1081 cgcataattc gaaaagcatt cccgcttttt gttgtgaagc tcaacgtgcc gtggtccgga
     1141 gtcgtcgtag ggccactcgg cggcgccgta gtgaatggcg gccatgtacc aattgtcgcg
     1201 cgcggtgacc ggattgccgt ccgcctcggc ggcgcgggcc ttggcctcgc gacgtttcgc
     1261 cgtgctttcg aacgccgggc cgatgtcggc cattttttgc acgcgcgcgc gaatggcggc
     1321 gaaatcggca ttggcttcca cgccgcaggg cgcggataga tagatcgatc gcggttgatc
     1381 ccagtcgatg ccattggctc gaatgatgtt gtcgagcgcc caacgctgct gcgagaagcg
     1441 cttggtcgtg gccgcgacct ctgcctcggc gcatgcaacg cgatgcgaga ctgcgagcgc
     1501 cgctccggcg cctgccatag caaccgaacg aatgaagccg cgtcgatcca tgaccatacc
     1561 tccgatgctt cgttggcttc tcaatggcgc gcgtgaaggt gcaatggtaa tttagcagtg
     1621 gccatcacgc caggtgtgcg ccgccaatga ggatggagtc gctcggcacg ctcaccgtgg
     1681 ggattgaggt tcgcaacgga tcatggaccg cgattgcgcg gccgcacgag cgcggcggtc
     1741 gagcggactt ggtgctgggc gtcatcgctt cgtccgcgat aatccgctag aactgaatgc
     1801 tcctcggccg cgccgcaaat cgacggagga aagcgccatg cgaatgatcg acctgagccg
     1861 cgaactctat catcgcaccc ccacctatcc ggggcagccg ccgatcatcc acggcgtctg
     1921 gaaaacccac gaggagtcct tcgtcgaatc cggcaacgtt cacggcaatt gcgtgatgtt
     1981 tttctccatg cccgaccatg gcggcaccca catcgatgct ccccgccact tcggccagaa
     2041 cgggacgccg atcgacgaat atccgctcga gtactgctac gtgaagggaa tttgcctcga
     2101 tctgcgccac atggcgccga aggccgaaat cacgccgtcg cacctggagg aggcggtgcg
     2161 caagtcgggc gccgccatcc c
//
LOCUS       DATLJT010001323        11298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3785072,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001323 DATLJT010000000
VERSION     DATLJT010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3785072"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="fabD"
                     /locus_tag="VM910_30350"
     CDS             <1..867
                     /gene="fabD"
                     /locus_tag="VM910_30350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVI66844.1"
                     /translation="ARAVFDEVDAALGEKLTAVIWEGPAETLQLTENAQPALMAVSIA
                     TLRVLETEAGFSVGRDAAFVAGHSLGEYSALAAAESLTVADTARLLRIRGLAMQKAVP
                     VGAGAMAALLGLDYETAVQVADEAAQGQVCQAANDNGGGQVVVSGDKAAVDRAVEIAK
                     SKGARRAMLLPVSAPFHCRLMQPAADAMAKALSDVTIRKPVSPLVANVLASAISDPDE
                     IRLRLIEQVTGTVRWRESVAYMASRGVDRFFEIGAGKVLSGLVKRIADGAVGVAVGGP
                     NDIAAAKEALMR"
     gene            968..1705
                     /gene="fabG"
                     /locus_tag="VM910_30355"
     CDS             968..1705
                     /gene="fabG"
                     /locus_tag="VM910_30355"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HVI66845.1"
                     /translation="MFDLTGRTALVTGATGGIGGAVAKALHAQGATVAISGTRREVLD
                     KLAGELSERAHVLPCNLADTAEVEALVPAAEKTMGQLDILVANAGITRDNLFVQLRDE
                     DWDEVINVNLTATFRLARAATKLMMRKRFGRIIAITSVVGVTGNPGQANYTASKAGLI
                     GLIKTLGAEYARRNVTANCIAPGFIKTPMTDALNEKQREAILARVPAGRLGTPEDVAA
                     AAVYLASNEAAYVTGQTIHINGGMAMI"
     gene            2063..2302
                     /locus_tag="VM910_30360"
     CDS             2063..2302
                     /locus_tag="VM910_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVI66846.1"
                     /translation="MSEIGERVKKIVVEHLGVEPEKVVDNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS"
     gene            2393..3658
                     /gene="fabF"
                     /locus_tag="VM910_30365"
     CDS             2393..3658
                     /gene="fabF"
                     /locus_tag="VM910_30365"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVI66847.1"
                     /translation="MRRVVVTGLGMVSPLGCGVEPTWKRILNGESGARRIETFDVSDL
                     ASKIACQVPRGDGSDGTFNPDQWMEPKDQRKVDDFIIFAMAAARQALDDANWHPASEE
                     DRCATGTMIGSGIGGLSGIAETALLLKERGPRKVSPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDAARLIALSDADVMVAGGTESPISRIGIAGFCAARALSTA
                     FNDTPARASRPYDRDRDGFVMGEGAGIVVLEEYEHARARGAKIYAEVTGYGLSGDAYH
                     ITSPSPDGDGGFRSMTAAMKRAGITSADIDYINAHGTSTQLGDEIELAAVERLVGNAA
                     SRVSMSSTKSSTGHLLGAAGAIEAIFSILAIRDNIAPPTINLEHPSVETAIDLVPLVP
                     RKREINVALSNSFGFGGTNASVIVQRVIN"
     gene            3780..5051
                     /gene="mltG"
                     /locus_tag="VM910_30370"
     CDS             3780..5051
                     /gene="mltG"
                     /locus_tag="VM910_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVI66848.1"
                     /translation="MSERPPISPRSPRAALEPEQVPPPPRRSERARNPFVIVGNAIIT
                     IVLFLMIGTGAAYYYGKQTIEAPGPLQEEKVVNIPSRAGMTDIADVLQREGVIDNNRW
                     AFIGAVFALKARSELKPGEYAFKEHASLRDVIGTIVEGKVVQHSVTIPEGLTSEQIVA
                     RLSDNDIFSGTVREIPREGTLLPETYKFPRGTAREQVVQRMQAAQRRVVAEIWERRSA
                     DLPLKSPEQLVTLASIVEKETGRADERSRVAAVFVNRLKQKIKLQSDPTIIYGLVGGK
                     GTLGRPIKRSEITQPSPFNTYVIDGLPPGPISNPGRASLEATANPARTRDLYFVADGT
                     GGHAFTETYDQHQKNVAKLRATEKQTQNDTVEPADDPPTAATPAESAPPATPAAKPPA
                     AKKPPRAPAATRQGPAQSTATAPPGAPVVQQ"
     gene            5143..6030
                     /locus_tag="VM910_30375"
     CDS             5143..6030
                     /locus_tag="VM910_30375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HVI66849.1"
                     /translation="MALSSMTGFARSHGASGPYAFEWELKSVNAKGFDLRLRLPQGWD
                     ELETHAKKRAGEMLARGTVYATLNVKRADALSTVRINEDVLASIVKVASQLAGKIDAV
                     APSIDGLLGIKGVIEVVEPERNEQEDRAAKQAAASAFDEALANLVTMRQREGAALGQI
                     LTQRMDEMEQLASKAEAAPGRKPEAVRARLAEQVALLLEATDRFDADRLTQEALLIAA
                     KADIREELDRIASHISQARELIGKGGSIGRRLDFLAQEFHREVNTCCSKSNDVELTNT
                     GLEMKSVVEQFREQVQNLE"
     gene            6050..6709
                     /gene="gmk"
                     /locus_tag="VM910_30380"
     CDS             6050..6709
                     /gene="gmk"
                     /locus_tag="VM910_30380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVI66850.1"
                     /translation="MTAHEHGFDGVERRGLMFVLSSPSGAGKTTLSRMLIERMPGLKM
                     SVSATTRPKRPGEVEGRDYLFVDKARFEQMVKRGELLEWATVFDNRYGTPRAPVEAAL
                     SKGEDVLFDIDWQGTQQLREKARADVVSVFILPPSAADLEKRLHSRAQDSGQVIRSRM
                     SRASHEMSHWAEYDYIVINHDVDAAFAEVQSILKAERLKRERRVGLTTFVRELQRQLE
                     I"
     gene            complement(6716..7684)
                     /locus_tag="VM910_30385"
     CDS             complement(6716..7684)
                     /locus_tag="VM910_30385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66851.1"
                     /translation="MSNKPVKFASAIFVALFLSTTVFVGTGRAADDCLTEPNGATPQG
                     KHWFYRIERGSGRHCWYLRGEDEKSARAATPDSASPAPAPPPRGMETSNPRAIADAHA
                     EWPTRTPVGQDPGAATAPAASPPAEVTGSLPPDNAPRAQVVTRWPDSSAVLPSLNSPP
                     QTSGAAPDVQADATTSEPPPATVPPPPAQAMMPMERNIGSLQKLLLVAFGALALAGLT
                     GSAVYRLAGARRQARARRDRWASRTAQKLVREKLMRDPNIPTMAPWVEPNIGRTAPVR
                     DTSDADEPVDVPNERVERIEDFLARLTRQLQAELDGPCAERQRAAN"
     gene            complement(8012..8254)
                     /locus_tag="VM910_30390"
     CDS             complement(8012..8254)
                     /locus_tag="VM910_30390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66852.1"
                     /translation="MSDDNMKAELERLRRENEALKKGASDGIRMKVSEKGGVSVYGMG
                     RFPVTLYKEQWLKLLDMSDEIRAFIAENEGRLKAKD"
     gene            8446..8742
                     /locus_tag="VM910_30395"
     CDS             8446..8742
                     /locus_tag="VM910_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66853.1"
                     /translation="MYIVIRKYTKVRSVADAARRAKSGVGQILRKSRGFRSYYVLDGG
                     EGVGVAVMIFEDRESANAANDKVLEFVQASLHDLDLGDPEIIAGEVLVNIESDA"
     gene            8886..9074
                     /locus_tag="VM910_30400"
     CDS             8886..9074
                     /locus_tag="VM910_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66854.1"
                     /translation="MSDRTSKDRVQREAARTFQHAGKRKAETDYEKAQEAFWKNYERL
                     KAERLAREAAKSEDKKQR"
     gene            9450..10007
                     /locus_tag="VM910_30405"
     CDS             9450..10007
                     /locus_tag="VM910_30405"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HVI66855.1"
                     /translation="MKDFVYVISGEHGRRRIGCSDNPRQRLKELQTGSPYPLKFEFIG
                     EAENKQIELEAYFMLNQHKSPGDDEWFTVPPDVAVTTVMAAANRLGYRIKQVDPDAVA
                     PPNSRRQPLWVTTAMLPFVAAYAYWIFEIAYRHLESGEIPMETFVIEALVLGVALVIL
                     RSLIRWFGNQLIALWQAWLRLMNPG"
     gene            complement(10037..11247)
                     /locus_tag="VM910_30410"
                     /pseudo
     CDS             complement(10037..11247)
                     /locus_tag="VM910_30410"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         2199 a         3407 c         3627 g         2065 t
ORIGIN      
        1 gctcgtgccg tatttgacga ggtcgacgcg gcgctcggcg aaaagctcac ggcggtgatc
       61 tgggaaggcc ctgcagaaac cctgcagctc accgaaaatg cccagcccgc gttgatggca
      121 gtttcgatcg ccaccttgcg cgtgctggag accgaggccg gcttttcggt cggtcgcgac
      181 gcggcctttg tagccggcca ttcgctcggc gaatattcgg cgctcgctgc tgccgaaagc
      241 ctcacggtag cagacacggc gcgactcctg cgcatccgcg gcctcgccat gcagaaggcg
      301 gtcccggtcg gcgccggcgc catggccgcg ctgctcgggc tcgactacga gacggcggtc
      361 caggtcgccg atgaagctgc ccaagggcag gtctgccagg ccgccaacga caacggtggc
      421 ggtcaggttg tggtctccgg cgacaaggct gccgtcgatc gcgcggtgga gattgccaag
      481 agcaagggcg ccaggcgcgc gatgcttctg ccggtatcgg cgcccttcca ttgcaggctg
      541 atgcagccgg cagccgacgc gatggcgaag gcgctttccg acgtcaccat ccgcaagccg
      601 gtatcgccgc tggtggccaa cgtactggca tccgcgatca gcgatcccga cgagatccgc
      661 ctgcgtctga tcgagcaggt aaccggaacc gtgcgctggc gcgagtcggt cgcctatatg
      721 gcaagccgcg gcgtcgaccg tttcttcgaa atcggcgccg gcaaggtctt gagcggcctc
      781 gtcaagcgga ttgccgatgg cgccgtgggt gtggccgtcg gcgggcccaa tgatatcgct
      841 gccgcaaaag aggcgctaat gcggtgaaat tggcgtccgc tgacattcgc aagagagttc
      901 gccgcgagca gctacgaatg ttagaatcag gacaccaatg ccgcgcaatc ggcgtcagga
      961 gacttccatg ttcgacttga ctggcaggac ggcgctcgtc accggtgcga cgggcggtat
     1021 cggcggcgcg gtcgcgaagg cgctgcacgc gcagggcgcc acagtggcga tctcgggcac
     1081 gcggcgcgag gtgctggata agctcgccgg cgaattatcc gaacgcgcgc atgtgttgcc
     1141 ctgcaatctt gccgatactg ccgaggtcga ggcgctggtg cccgcggccg agaagaccat
     1201 gggtcagctc gacattctcg tcgccaacgc cggcatcacg cgcgacaatc tgttcgtgca
     1261 gcttcgcgac gaagactggg acgaggtcat caatgtcaat ctgacagcaa ccttccgcct
     1321 cgcccgggcc gccaccaagc tgatgatgcg caagcgcttt gggcggatca ttgccatcac
     1381 ctcggtggtc ggcgtcaccg gcaatccagg acaggccaat tacaccgctt cgaaggcggg
     1441 actgatcggc ttgatcaaga cgctgggcgc cgaatatgcc agacgcaacg tcaccgcgaa
     1501 ttgcatcgcg ccgggcttca tcaagacgcc gatgacggat gcgctaaacg aaaagcagcg
     1561 cgaagcgatt ctcgcgaggg tcccggcggg acggctgggc acgcccgaag atgtcgccgc
     1621 ggcggcggtc tatctggcgt ccaacgaagc agcctatgtc acggggcaga cgattcacat
     1681 caacggcgga atggccatga tttaaagggc ccgcacggcc tcgaggatgc ggcgaaaggc
     1741 cgttttggac ggcagcgcgg ggcttatggt caaggctttt gaagtatgat accgaacccg
     1801 gaaacggatg ggcgaagaac gccattgcag gattcctaaa ccctgtatat tggcgaggcg
     1861 gagcctgccc gtcgttcgcc ggcccgcttc ggctggcgcc acgttttgag ctgcgccgcc
     1921 tgccacgacg gttcagacct tgaaagcggg acgctggaac ggggccaaat caggtttatg
     1981 cgcggttgcg aaggaagtct aacgaccacg atggctcgca tcatcctggg tcgggtcaag
     2041 gaaacaagca cgaggttgaa cgatgagtga gattggcgaa cgggttaaga agatcgtggt
     2101 cgaacacctt ggtgttgaac ccgaaaaagt ggtcgacaac gcgagcttca ttgacgacct
     2161 cggcgctgac agtctcgaca ccgtcgagct tgtgatggct tttgaagaag agtttggttg
     2221 tgaaattccc gacgacgccg ccgagacgat tctgaccgtc ggcgacgcca ccaaatttct
     2281 cgaaaagaac gcaaagagct gacgcgtttg cggtttcgaa acccgacggg ccgtgcgtga
     2341 gcggtccacc ggttttgtgc gagcctgccc gtcgctgcct ggagttgagg acatgaggcg
     2401 agtcgtcgtc acaggcctcg gcatggtttc gccactcgga tgtggcgtcg agccgacctg
     2461 gaagcgcatc ctcaatggcg agagcggtgc aaggaggatc gaaactttcg acgtctccga
     2521 tctggcgagc aagatcgcct gccaggtgcc gcgcggcgac ggaagcgacg gtacgttcaa
     2581 tcccgaccag tggatggagc cgaaggacca gcgcaaggtc gacgacttca tcatctttgc
     2641 gatggccgcg gcgcgccagg cgctcgacga cgccaactgg catcccgcaa gcgaagagga
     2701 ccgctgcgcg accggcacga tgatcggctc gggcattggc ggcctctccg gcattgccga
     2761 aaccgcgctg ctgctgaaag agcgcgggcc gcgcaaggtg tcgcctttct tcatccccgg
     2821 ccgcctgatc aatcttgctt ccggctacgt ctcgatcgag cacggcctca agggcccgaa
     2881 tcacgccgtc gtcacggctt gctcgaccgg ggcccatgcg atcggtgatg ccgcccggct
     2941 gattgcgctg agcgatgccg atgtgatggt tgcgggcgga acggaatccc cgatcagccg
     3001 catcgggatt gccggatttt gcgcggcgcg cgcactgtcg accgcattca acgacacgcc
     3061 tgcaagggcg tcccgaccat atgacaggga tcgcgacggt ttcgtgatgg gcgagggcgc
     3121 cggcatcgtg gtgctcgaag agtacgagca tgcgagggcc cgcggcgcga aaatctacgc
     3181 ggaggtgaca ggctatggcc tgtccggcga tgcctatcac atcacctcgc cctcgccgga
     3241 cggcgacggc ggcttccgca gcatgactgc cgcgatgaag cgcgcgggta ttacatccgc
     3301 cgacatcgac tacatcaacg cgcacggaac ctcgacgcag ctcggcgacg aaatcgagct
     3361 ggcagccgtc gaacggctgg tcggcaatgc cgcatccagg gtctcgatgt cctcgaccaa
     3421 atcctccacc ggccatctgt taggcgcggc gggcgcgatc gaggcgattt tttctattct
     3481 cgcgattcgc gataatatcg caccgcccac catcaacctg gaacatccgt ccgtggaaac
     3541 ggcgatcgac ctcgtgccgc tggtgccgcg caagcgcgag atcaatgttg cgttgtccaa
     3601 ttcgtttggt ttcggcggca cgaacgcatc ggtgatcgtc cagcgtgtga tcaactgatg
     3661 aaggtcaccg agaatccacc gtttcgccgc attcggtgac gaatcgtagg ttcgggcgca
     3721 gacttaaggc ccctcgcaag caggcgaggc cgcgatgaga gccacaggat tcaggttgca
     3781 tgagtgaaag gccgcccatt tcgccccgca gcccgcgcgc cgcgctggag cccgagcagg
     3841 ttcccccgcc gccgcggcgg tcggagcgtg cgcgcaaccc gttcgtgatc gtcggcaacg
     3901 ccatcatcac gatcgtgctg ttcctgatga tcggcacggg cgcggcctat tactacggca
     3961 agcagacgat cgaggcgccg ggaccgctgc aggaggagaa ggtcgtcaac attccctcgc
     4021 gcgccggcat gaccgacatc gccgacgtcc tgcagcgcga gggcgtgatc gacaataatc
     4081 gttgggcctt catcggtgcg gtgttcgcgc tgaaggcgcg gtcagaattg aaaccgggcg
     4141 aatatgcctt caaggagcac gcatccctgc gcgacgtcat cggtacgatc gtcgagggca
     4201 aggtcgtaca gcattccgtg accatcccgg aggggctcac atccgagcag atcgtggcac
     4261 ggctgagcga caacgatatt ttctcaggga ccgtccggga aataccgcgt gaaggtacgt
     4321 tgttgccgga gacctacaaa ttcccgcgcg gcaccgcgcg cgagcaggtg gtccagcgga
     4381 tgcaggcggc gcagaggcgc gtcgtcgccg aaatctggga gcgcaggagt gccgatctgc
     4441 cactgaaatc gccggaacaa ctggtgacgc tggcctccat cgttgagaag gagaccggca
     4501 gggccgacga gcgcagccgg gttgcggcgg tgttcgtcaa tcggctcaag cagaagatca
     4561 agctgcaatc ggatccgaca atcatctacg gcctggtcgg cggcaaggga acgctggggc
     4621 ggccgatcaa gcggagcgag atcacgcagc cctcgccctt caacacctat gtgatcgacg
     4681 gcctgccgcc ggggccgatc tcaaatcccg gccgcgcctc gcttgaagcc accgccaatc
     4741 cggcgcgcac ccgcgacctc tatttcgttg ccgacggcac cggcggtcac gctttcacgg
     4801 aaacttacga ccagcatcag aagaacgttg ccaaattgcg cgcgacggag aagcagaccc
     4861 agaacgatac ggtcgagccc gccgacgatc ctccgaccgc ggcgacgccg gccgaatcgg
     4921 cgccgccggc caccccggcc gccaagcctc ctgccgcgaa gaagccgccg cgcgcgccgg
     4981 ctgcaacccg acaggggcca gcgcagtcga cggcgacagc gccgcccggg gcgcccgtcg
     5041 tccagcaata gctgcgggac tccacatttc ctgaaaaggt cttatggtcc gcgcgcgttt
     5101 ggcccgcgaa tctgagactg ttgccatcgg agaactgacg ccatggcgtt gtcgagcatg
     5161 accggctttg cccggagcca cggggcaagc gggccctacg cattcgaatg ggagttgaag
     5221 tcggtcaatg ccaagggctt cgacctgcgc ctgcgtctgc cgcagggatg ggacgaactc
     5281 gaaacgcatg ccaagaaacg cgcgggagag atgttggccc gtggcacggt ctacgccact
     5341 ctcaacgtca agcgcgcgga tgcgctctcg acggtacgaa tcaatgagga cgtgctcgcc
     5401 tcgatcgtga aggtcgcgag ccagcttgcc ggcaagatcg acgcggtggc gcccagcatc
     5461 gacggccttc tgggcatcaa gggcgtcatc gaagtcgtcg agccggagcg caacgagcaa
     5521 gaggaccgcg cggccaagca ggcagcggcc agcgcattcg acgaggcact cgcgaatctc
     5581 gtgacaatgc ggcagcgcga gggcgctgca cttgggcaaa ttctcaccca gcgcatggat
     5641 gaaatggagc agcttgccag caaggccgaa gccgcgccgg gccgcaagcc ggaggccgtc
     5701 agggcgcgcc tcgccgaaca ggtggcactg ttattggaag cgaccgaccg tttcgatgcg
     5761 gaccgcctca cccaggaagc gctcttgatc gcggccaagg ctgatatccg agaagagctc
     5821 gaccgcatcg cctcgcatat ctcgcaggcg cgcgaactga tcggcaaggg cggatcgatc
     5881 ggacgccgac tggatttcct ggcgcaggag tttcatcgcg aggtcaacac ctgctgctcc
     5941 aagtcgaacg acgtcgagtt gaccaacacg gggctcgaaa tgaaaagtgt ggtcgagcaa
     6001 ttccgcgagc aggtgcagaa tctggagtga tccgaatctg gagcgaatca tgacggctca
     6061 cgaacacggt tttgacggcg tcgaacggcg cggattgatg ttcgtgctgt cctcgccctc
     6121 gggcgcgggc aagaccacgc tgtcgcggat gctgatcgag cgaatgccgg ggctgaagat
     6181 gtcggtatcc gccaccacgc ggcccaagcg ccccggcgaa gtcgaggggc gcgactatct
     6241 gttcgtcgac aaggcccgct tcgagcagat ggtcaagcgg ggcgaactgc tggaatgggc
     6301 gaccgtgttc gacaatcgct atggtacgcc gcgggcgccg gtggaggccg cgctgtcgaa
     6361 aggcgaggac gtgctgttcg acatcgactg gcagggtacc cagcaattgc gcgagaaggc
     6421 gcgcgccgat gtggtcagcg tcttcatcct gccgccgtcg gccgccgatc tggaaaagcg
     6481 cctgcactcg cgcgcgcagg attccggtca agtgatccgc tcgcgcatga gccgcgcgag
     6541 ccacgagatg agccactggg ccgaatacga ctacatcgtg atcaaccacg acgtcgacgc
     6601 ggccttcgcc gaagtgcagt cgatcctcaa ggccgagcgg ctgaagcggg agcgccgggt
     6661 gggactaacc accttcgtgc gcgagctgca gcggcagctc gagatttagt ccgcgtcaat
     6721 ttgcggcgcg ttggcgctcc gcacatggac cgtccaactc ggcctgcagt tgccgtgtca
     6781 accgcgccag aaagtcctcg atgcgttcga cccgctcgtt cgggacatcg acaggttcgt
     6841 ccgcgtcaga ggtatcgcgc acgggtgctg tcctgccgat gttgggttcg acccatggcg
     6901 ccatggtggg aatgttcgga tctcgcataa gcttttcacg gacaagcttt tgcgcagtcc
     6961 tcgacgccca gcgatccctg cgcgcccggg cctggcggcg agcgcccgcc aaccggtaaa
     7021 cggcgctgcc ggtcagcccg gcaagcgcca gcgcgccaaa tgcgaccagg agcagtttct
     7081 gcagcgaacc gatgtttcgc tccatcggca tcatcgcctg cgcgggcggc ggcggaaccg
     7141 ttgcgggagg aggttctgag gtcgtcgcgt cggcttgcac gtccggcgca gcgccggagg
     7201 tctggggagg cgaattgagc gatgggagaa cggcggagga atcgggccag cgcgttacga
     7261 cctgggctcg cggcgcattg tccggcggca acgagccggt cacctccgcg ggaggcgaag
     7321 cggccggcgc ggtagccgcg cccggatcct ggccgacggg agtccgcgtg ggccattcgg
     7381 cgtgggcatc ggcaattgcg cgggggtttg aggtctccat cccgcgtggc ggcggtgccg
     7441 gtgccggcga agccgagtcg ggtgtagccg cgcgcgcgga cttttcatcc tcgccgcgca
     7501 aataccagca gtgacgcccg gatccgcgct cgatgcggta aaaccaatgc ttgccttgcg
     7561 gtgtcgcgcc attcggctcg gtcaggcagt cgtcggccgc gcgcccggtg ccaacaaaga
     7621 ctgtcgtcga caggaaaagg gcgacaaaaa tggccgatgc gaatttgacc ggcttgttcg
     7681 acatacgcat ctcccactac gcgacgcaac acaccctacg acatcccagt ctcgacaaag
     7741 acttgggtcg caataggccg caattccgga gaggatgggg cttaattcgg gctcgccgtt
     7801 tgacgcggcg cgtacttcgg caaacgaaag gcagcgcgat ttgccgcgtc acggttgaat
     7861 agcctggccc gggcaatcac gccgcgtggc gttttggcga aacctgggat tgtccgccag
     7921 ggatttggga ttgcccgcca gggatgaagt acgacaaagc actcgttcga gccggaggcc
     7981 tcggggccga aaaacgatcg ctcgtccgct atcaatcttt cgccttcagc cgcccctcgt
     8041 tttccgcaat aaacgcacga atctcgtcgg acatgtccag aagcttcaac cattgctctt
     8101 tatataacgt cactggaaag cgccccattc cgtaaactga gacaccaccc ttttcgctta
     8161 ccttcatgcg gatgccgtcg gaggctcctt tcttgagagc ctcgttttcc ctgcggagac
     8221 gttcgagttc agctttcata ttatcgtcgg acatggcatt tccttttcgc tagcttgatt
     8281 ttatcacgcc gtgctcgccg ttcatacctt gactgtccgc ttttgggcgg agtcgacctg
     8341 ccttcaggct cggctgatgt ctgcaattga cacatctcgg aagtagggcc tagtccagca
     8401 gcggcacgat atgctttgat gcgtgggctg gaaccgagac gctacatgta catagtcatc
     8461 cgaaagtaca caaaggttcg ttcggttgct gatgctgctc gccgcgccaa aagcggcgtc
     8521 ggtcagatcc tgaggaaatc gcgcgggttc agatcttatt atgtactgga tgggggcgaa
     8581 ggcgtcggcg tggctgtgat gatattcgag gaccgcgaaa gcgccaatgc ggcgaacgat
     8641 aaagtgctgg agtttgttca agcaagcctg cacgatcttg atctcggaga ccccgaaatc
     8701 atcgccggcg aagttttggt gaatatagaa tctgatgcgt aattgttctt gaaggctgct
     8761 catccgttac gtgcggccaa attgctgcaa agtcgccaac attaaactta atcagcgagt
     8821 ttccttgcga cacaccccgg ctgagctatc gtcattttac gggtcaccga aattggatgt
     8881 gatctatgag cgatagaaca agtaaggatc gcgtgcaacg agaggcggca aggacttttc
     8941 agcacgccgg taaacggaag gcggaaacgg actacgaaaa agcgcaggaa gccttctgga
     9001 aaaattacga acgattgaag gcggaacgct tggccaggga ggcggcgaag tccgaagaca
     9061 aaaagcagcg ttgaccagcg tttccgcttc aaaccagtcc ggccgtcagg ggatagccgc
     9121 gctattttta ttgcggcaga tcaaacttgc cttggcagac tggcgtatcg ttatgattgg
     9181 accgcgatag gtctcccacg ccagcagtgc cagagcgggt cgctcccgcc agttgccagt
     9241 cgaagtgggc cggtccacgc gttagtgcac taggaacggc gctggtgaca gcgccgtttc
     9301 tcgttggcgc cggcgtcata agcgaggagg cgacgccccg tgcattccgc cgacattaaa
     9361 cttaattggc aaagctaatt ggctagtttc cttgcagcac accccggctg agctaccgtc
     9421 atcttcagat cactaaaatt ggatttgaaa tgaaggattt tgtttacgtg atttccggag
     9481 agcatggtcg gcggaggatc ggatgctccg acaacccgag acagcgcctg aaagaactcc
     9541 agaccggctc gccctacccc ctgaaattcg aatttatcgg cgaggccgag aacaagcaga
     9601 tcgaactcga agcgtatttc atgttgaacc agcacaagtc gcccggcgac gatgaatggt
     9661 tcacagtgcc gcccgacgtc gcggtgacca ccgtaatggc ggccgccaac cggctcggtt
     9721 accgcatcaa gcaggtcgac cccgacgcgg ttgcgccccc caactcgcgc aggcagccat
     9781 tgtgggtcac caccgccatg ctgccgttcg tcgccgccta cgcctattgg atattcgaga
     9841 tcgcctatcg ccatttggaa agcggcgaga taccgatgga gaccttcgtg atcgaggccc
     9901 tggtgctcgg cgtagctctc gtgatcctgc gatccttgat caggtggttt ggcaaccaat
     9961 tgatcgcgct ctggcaggcg tggctgcgcc tgatgaaccc gggctagagc gaggccggtc
    10021 tatccgtcat ccggcattac tcgctcgcca gcacgtcgct gaacaggacg taacgctggc
    10081 cgtcgaaacg caccagccgc atttgcttga tcggccggtg gtccgtcggg ctcgaggtca
    10141 atctgatgcc cggcaggagc agcggcaggt ccatgttcag gtgggtggcc ttctccatga
    10201 cattctcgcg cgtgagattg tcgccgcatt gcttcaatag ctccaccacc gcgtggccga
    10261 cgttgtagcc atagggcgcg taatagtcgc tcttgtcgac atgcggattg tatttgtcca
    10321 tccagatatt ccaggccttc atcgcggggt cgtcacgcca ttgcggatcg gccggatcct
    10381 tctggtaggc cgccgagatg atgcccttgg cggcctcgaa gcccgccggc cggattgcgc
    10441 ccgcgatcga ggagccgacg cttgagacga attcctgcgg gtgccagtcg atgtcgtagg
    10501 ccttgcggat cgcctgcgct gcgaatttcg gcaccgaaaa gatcagaagc acgtcggcgc
    10561 cggaagcctt cagcgacacg atctgcgagt ccacggtggg atcgctggtg ttgtaggttt
    10621 gctgcgacac gatcatcttg tcggtcagtt cgccaagccc gcgcctgaag cccaggagat
    10681 agtcgcggcc gagatcgtcg ttttgcgaga gaataccgac cttggcgtcg ggcttgttgg
    10741 tgagaatcca tctggcgtag atctcaccct cggatgcgta gtgaggagtc cagctcagcg
    10801 tccacggata gtgctcggga tcgttgaaga cggtcgctcc ggtgccgatg aagaggtgcg
    10861 gcaccttttt ctggttgatg tatttcacga ccgccatgtt ggtcgcggtg ccgacgggat
    10921 tgaccaggaa cagcgcttcc tcgctctcga tcagacggcg gatctgctcg acggtcttgg
    10981 gcggctatag gcgtcgtcga gggcgacata gttgatcttg cgcccgttga cgccaccctg
    11041 ttcgttgatc atcgcaaaat aggccgttgc ggagacggcg ccggccgaat aggcggaagc
    11101 cgggccgctg aagggggtcg tcgtgccgat cttgatctcg gtatctgtga cgccgggacc
    11161 gtacttcttc tgggccagtg ccggtggcgt cgcaaacgcc agcgcgagcg cagcgacgac
    11221 gcctatcccg gatttcctcc aagccatgat gtcctcccgc tctggccggc gaggccggcg
    11281 agactttttt gtttcagt
//
LOCUS       DATLJT010001324         1527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1026400,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001324 DATLJT010000000
VERSION     DATLJT010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026400"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VM910_30415"
     CDS             <1..665
                     /locus_tag="VM910_30415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66856.1"
                     /translation="TLYDKLNYNFRDVAPVSAITRAPIVLLVNPTFAPKTVPEFIAYA
                     KANPDRINYASAGVGGSFHLSGELFKAMAGVRLVHVPYRGAAPGLTDLIGGQVQAMFV
                     DTPPSIEHIKSGKVRALAVTTATRSELLPELPTVADFVPGYEASLWNGVCAPKGTPAE
                     IVERLNREINAGLADPKLKGRLAEMGAWPLPGSPADAGKLINDEIEKWAKVIRSGNIK
                     PE"
     gene            complement(755..>1527)
                     /locus_tag="VM910_30420"
     CDS             complement(755..>1527)
                     /locus_tag="VM910_30420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66857.1"
                     /translation="YAALGVPPDTEDALVRWQRLMPKPEPEPKARRLDTPQVTFDDVD
                     QRVGQAIAAEHQFIMDILTETVAHLQDEIKKEAAGKPGPRGEQGPPGKLPLVRLWKPE
                     TVDYEGDVVAYDGGTFQAQRDTGQAPLHSDWICLAAPGRDGRSVTVRGTFDEAADYQR
                     LDIVAFNGGSFIALKDAPGHCPGPGWQLFASPGKRGIAGPKGERGERGPTGLSGATIR
                     DWKIDRARYVATPVMSDGREGPPLELRGLFEQFLADAG"
BASE COUNT          275 a          509 c          464 g          279 t
ORIGIN      
        1 ccacgctcta cgacaagctc aactacaatt tccgcgatgt cgcgccggtc tcggccatca
       61 cgcgcgcacc catcgtttta cttgtgaacc caacgtttgc ccccaagacg gttcctgaat
      121 ttattgctta tgctaaggcc aaccccgata ggatcaatta tgcctcggcc ggcgttggag
      181 ggtcttttca tctctctggc gagctgttca aggcaatggc aggcgtgcgc ttggtccacg
      241 tgccttatcg cggggcagca cctgggctga ccgacctaat cggtgggcag gtccaggcga
      301 tgttcgtcga tacgccgcca tcgatcgagc acatcaagtc cggtaaggtc cgcgccctgg
      361 cagtgacaac ggcgacgcgc tcggagttgc tgcccgagct gccgacggtc gccgacttcg
      421 tgcccggcta cgaggcgagc ctgtggaacg gggtgtgcgc gccgaagggc acgccggccg
      481 agatcgtcga gcggttgaat cgggagatca acgccggcct tgccgatccc aagctcaagg
      541 gaaggctggc cgagatgggg gcctggccac tgcccggctc gcccgccgac gccggcaagc
      601 tgatcaatga tgaaatcgag aagtgggcga aggtgatccg ctccggcaac atcaagccgg
      661 agtagtccga gcggctccat attccataac ctcaccgtta ggcgaacccg gtactcaccc
      721 cgcgcgctga tgtcgtctta gcccgcgcac atcgctaacc cgcgtcagcc aagaactgct
      781 cgaacagccc gcgcagttcg agcggcgggc cctcgcggcc gtccgacatc acgggcgttg
      841 caacgtagcg cgcgcgatcg atcttccagt cgcgaatcgt cgcgccgctc aatcctgtcg
      901 ggccgcgttc gccccgctca cccttgggac cggcaatgcc acgcttgccc gggctcgcaa
      961 acagttgcca accgggacca gggcagtgac ctggcgcatc cttaagcgcg ataaagctgc
     1021 cgccattgaa ggcgacgata tcgagccgct gatagtcggc ggcctcatcg aaggtgccgc
     1081 gcacggtgac gctcctgcca tcgcggccgg gggctgccaa gcaaatccag tccgagtgca
     1141 atggcgcttg gccagtatcg cgctgggcct gaaacgtgcc gccgtcatag gcaaccacgt
     1201 cgccctcgta gtccacggtc tccggcttcc ataacctgac gagcggcagc ttgccgggcg
     1261 gcccctgctc accgcgcggt cctggcttcc ctgcagcctc ctttttaatc tcgtcttgaa
     1321 ggtgcgcgac agtctccgtc aaaatatcca tgatgaattg gtgctcagcg gcgatagcct
     1381 gcccgacgcg ctggtcgacg tcgtcaaacg tgacttgcgg cgtgtccagc cggcgcgcct
     1441 tcggctcagg ctcaggtttc ggcataagac gctgccagcg taccaacgcg tcctcagtgt
     1501 ccggcgggac gcctagagcg gcataga
//
LOCUS       DATLJT010001325         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1718180,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001325 DATLJT010000000
VERSION     DATLJT010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718180"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VM910_30425"
     CDS             complement(<1..936)
                     /locus_tag="VM910_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI66858.1"
                     /translation="MGVEGIGASVVRKEDKRFITGKGRYVDDIKLVGMTHAQFVRSPH
                     AHARVKSIDSSAAMKMPGVLAVLTGKELVDDKIGNLICGWAITSKDGSPMKMGAWPAM
                     APETVRFVGQAVAVVIAESKNLARDAAEAVVVDYEELPAIPDVRAAIKSGAPQLHPEA
                     PGNVVYDWVLGDEAAVNAAFSKAASVVTLELTNNRLVPNAMEPRAAIADYDAAEEHFT
                     LYTTSQNPHVARLVLSAFYNIAPEHKLRVIAPDVGGGFGSKIYIYPEEMVALWASKKV
                     GRPVKWTGDRSESFLTDAHGRDHVTKAEMAFDKDHK"
     gene            complement(1024..1509)
                     /locus_tag="VM910_30430"
     CDS             complement(1024..1509)
                     /locus_tag="VM910_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI66859.1"
                     /translation="MSTVKLTVNGKAVSVDCEDRTLLVHLLRDHLNLTGTHVGCDTSQ
                     CGACVVHLDGRAVKSCTMLAGQAAGSSVTTIEGIAKGDELHPMQAAFRDNHGLQCGFC
                     TPGMIMSAIDIVHRHGGKLDEATVRHELEGNICRCTGYHNIVKAVLDAAGRMKVAQAA
                     E"
     gene            1791..2201
                     /locus_tag="VM910_30435"
     CDS             1791..2201
                     /locus_tag="VM910_30435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66860.1"
                     /translation="MSKRGWSLTSKLPTLRFRAKVMLGFAAVLAISAVSLCIAYFGFE
                     HVSAAVASYRNSVAEADLARNIDRELIAYRAAARYYIVTGKEDDAKTALVAQTSLKEA
                     IDKSLAGTKNPGRLESVKRLGAEFKNFTDTLPKS"
     gene            2198..>2648
                     /locus_tag="VM910_30440"
     CDS             2198..>2648
                     /locus_tag="VM910_30440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVI66861.1"
                     /translation="MKVKQANEYLTRNAFSRNEMSSDYKLDELASAAAEAGLQDVEFQ
                     AKHIASQFQSIKIFSSTFVVSGDQAATTSTLSRLSFLDNQLKGVPSSNEKVAAVLNEV
                     NSLLKGYREAFSKMVENKKTIDDLVTRMGGSAGAIMQGASAMKADLVS"
BASE COUNT          485 a          813 c          808 g          542 t
ORIGIN      
        1 cttgtgatcc ttgtcgaacg ccatctccgc cttggtgacg tggtcgcggc catgcgcgtc
       61 ggtgaggaag gattccgagc ggtcgccggt ccacttcacg gggcggccta cttttttgga
      121 agcccacagc gccaccatct cttcgggata gatgtagatc ttcgagccga aaccgccgcc
      181 gacgtcgggg gcgatcaccc gaagtttgtg ctcgggcgcg atgttgtaga acgccgacag
      241 cacgagtcgc gcgacatgcg ggttctgcga tgtcgtataa agtgtgaaat gctcctccgc
      301 cgcatcatag tcggcgatcg ccgcgcgcgg ctccatcgcg ttcggcacca ggcggttgtt
      361 ggtgagctcg agggtcacga cactggcggc cttgctaaag gccgcattga ccgccgcctc
      421 gtcgccaagc acccagtcat agaccacgtt gccgggcgcc tcgggatgga gctgcggtgc
      481 gccggatttg atcgccgcgc ggacgtcggg gatcgccggc agttcttcat aatcgacgac
      541 gacggcttcc gccgcgtcgc gtgccagatt tttgctctcg gcaatgacga cggcgaccgc
      601 ctggccgacg aagcgaacgg tctccggcgc catggcaggc cacgcgccca ttttcatcgg
      661 tgaaccgtcc ttcgacgtga tcgcccagcc gcagatcagg tttccgatct tgtcgtcgac
      721 gagctccttg ccggtcagca ccgcaagcac gcccggcatt ttcatcgcag cggatgagtc
      781 gatgcttttg accctggcat gggcatgcgg gctgcgcacg aattgggcgt gggtcatgcc
      841 cacgagcttg atgtcgtcga cgtagcggcc cttgccggtg atgaaacgct tgtcttcctt
      901 gcgtacgacg cttgcgccga tgccttcaac gcccatgttc tctcctccct atcggagttc
      961 ttgcttccgc cacgcgcttg agccgcgatg gcctgaattt ttgcgttcga acgtcgcgag
     1021 cttctattcc gccgcctgcg cgaccttcat gcggcctgcc gcgtcgagca cggccttgac
     1081 gatattgtgg tagccggtgc agcggcagat attgccttcc agctcatgcc ggaccgtggc
     1141 ctcgtcgagc ttgccgccat gccgatgcac gatgtcgatt gccgacatga tcatgcccgg
     1201 cgtgcagaag ccgcattgca ggccgtgatt gtcgcggaac gccgcctgca tcgggtgcag
     1261 ctcgtcgccc ttggcaatgc cctcgatggt ggtcacgctg gagccggcgg cctgtcccgc
     1321 cagcatcgta caggatttca ctgctcgtcc gtcgagatgc acgacgcagg cgccacactg
     1381 gctggtatcg cagccgacat gagtgccggt caggttcaag tggtcgcgca ggagatggac
     1441 aagaagcgtg cggtcttcgc agtcgaccga gaccgccttg ccgttcaccg tcagtttgac
     1501 tgtcgacaca taagcctccc cgaagatttc ttgcttattc cgattagaaa cagggggcgg
     1561 gaactttgca actgggaatt tggcggtgcc ggtcattatg atgtcgcgat ccatgccctt
     1621 gcgcgttcag acaccgggac tgcgcgatgc aagcccggaa cggcttgatc gagggtgcgc
     1681 aggaggcccc tccgcacatt tactcacctt tagccaatct accctagttt cgcgcaccga
     1741 gccggcgacc cgcgtttccg gctgcgactg aatttggggt gggggtagga atgtcgaagc
     1801 gtggctggtc attgacctca aagcttccga ccttgcggtt tcgtgccaaa gtgatgctgg
     1861 gctttgccgc cgtcctcgcc atctccgccg tcagcctctg catcgcctat ttcggctttg
     1921 agcacgtgtc ggcggccgtt gcctcctacc ggaacagcgt cgccgaggcc gaccttgccc
     1981 gcaacatcga ccgcgagctg atcgcctatc gcgcggctgc ccgctattac atcgtgacag
     2041 gcaaggaaga tgacgccaag acggcgttgg ttgcgcagac gagcctgaaa gaggcgatcg
     2101 acaaatcgct cgccggcacg aagaacccgg ggcgtctgga aagcgtgaag cggcttggcg
     2161 cggagttcaa gaacttcacc gacaccttgc cgaaatcatg aaggtgaagc aggccaacga
     2221 gtatctgaca aggaacgcct tcagccgaaa cgagatgtcg tcggactaca aactcgacga
     2281 gctcgcaagc gctgcggcag aagccggatt gcaggatgtc gagttccagg cgaaacacat
     2341 tgccagtcag ttccaatcga tcaaaatttt ttcctcaacc ttcgtggtca gcggcgacca
     2401 agcggccacg acgagcactt tgagtcgtct gagcttcctt gataaccagc tcaagggcgt
     2461 cccgtcttcc aacgaaaagg tcgcggcagt cctcaacgag gtcaactcgc tgctcaaggg
     2521 ctatcgcgaa gctttcagca agatggtcga gaacaagaag accatcgacg atctggtgac
     2581 ccggatgggc ggttcggcgg gcgcgatcat gcagggcgct agcgcgatga aggcggatct
     2641 cgtttccg
//
LOCUS       DATLJT010001326         4374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2637573,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001326 DATLJT010000000
VERSION     DATLJT010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2637573"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VM910_30445"
     CDS             complement(<1..252)
                     /locus_tag="VM910_30445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66862.1"
                     /translation="MPTHAVGNTFRAIELEPQATAELEFSKFVEALLEKLRLLEEERF
                     VYYDDLRKKNAKWANGSRWLLALLGAIAFLLTGLVAALRF"
     gene            640..1113
                     /locus_tag="VM910_30450"
     CDS             640..1113
                     /locus_tag="VM910_30450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66863.1"
                     /translation="MGIFRRNVPIHYIPVHPDNITKKDWIILLEAGTIVGPSAEKLRV
                     ALATLEKKIVTKYNIFLKPEHFLVLEGYDPRIHALYYCPQLYKYGSDKKLVPLDGEEI
                     GRLVAKAVQNGVVEKQPWYPPSEMLPREPIVDGKMDIDFDSMVVDAQTSLSPTAR"
     gene            complement(1171..2325)
                     /locus_tag="VM910_30455"
     CDS             complement(1171..2325)
                     /locus_tag="VM910_30455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVI66864.1"
                     /translation="MRNSTIRVATFAGPGAEPVIRPVPWPQVPANAALIQIGACGVCG
                     TDLHILKGHWPKPLPWPFTLGHELGGIIVEKGGEFTEDFMGKKLGVGSKVMIPPLMPC
                     GQCYYCVHYPQQANKCLTPVYYGRYLGFDKPPHLWGGFAEYVYVDLDMLPGTKVYKLP
                     DDMPLRLGALSEPLTSCIRAFNRAVAARGFSWGDTVVIQGSGPIGILAIAAAREMGAG
                     RVICVGAPEKPRLELARKFGAEATVNIEEVKEPDQRIKRVRDIVGGFGADLVMDCSGH
                     PSAGPEGIEMLRDGGTYVEMGQFTDAGSIATSWHRICAKDLNVLGSWGFTANDLPLGV
                     DMLDRARDKYPWLDMQTLYPFDEDGVSRAVKDAMAMKTVKSTIVPWPELV"
     gene            2503..3102
                     /locus_tag="VM910_30460"
     CDS             2503..3102
                     /locus_tag="VM910_30460"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI66865.1"
                     /translation="MAGHLDFYFFYGSIHSYLSVMRIGPLASAARVEVRWQPFNLREI
                     LIEQNNTAFTKNDVKMNYFWHDVERRARRHNIPFAGRAPYPADPDLLALRVGLIAAQE
                     KWCDDYSRATFHEWFIARRAPGVDDHIERVLASLDKSPAPIIARAKGAEGERLLKAAT
                     DAARKLGIFGAPTFAIGAEIFWGDDRLEEALAFAGRTLI"
     gene            3192..3449
                     /locus_tag="VM910_30465"
     CDS             3192..3449
                     /locus_tag="VM910_30465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66866.1"
                     /translation="MANPGSNQETAAIEAAYRAQVELLFKSLITNLGDQVVSHQSDQQ
                     SLDKFKVGLNIAKRAKQLALNAVAPASPARAAASRRRKREE"
     gene            3675..>4374
                     /locus_tag="VM910_30470"
     CDS             3675..>4374
                     /locus_tag="VM910_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66867.1"
                     /translation="MRGKMFTRRRFMATSAAASTTMIAAPFVKTAHAAGKLSIGFWDH
                     FVPDANKTSDALIQEWAAKEKVEVQIDRFTGNKALITIAAEAQARSGHDILYMTSWLP
                     HAYASSLEPVDDVMIQLIKQNGPVNDIVAYLGRAESRWLAVPATIGNQIQGPCSRIDL
                     MKQYAGIDVQAMYPAGAPPKADNWTLDTFLKAAEACHKAGFPFGIGLGTTEDSVVAAG
                     VIFQCFGAMLVDANG"
BASE COUNT          885 a         1298 c         1341 g          850 t
ORIGIN      
        1 gaagcgcaac gccgctacca ggccagtcaa gaggaacgcg attgctccga gcaaggccag
       61 gagccatcga gagccgtttg cccatttcgc atttttctta cgcagatcgt cgtaatagac
      121 aaaccgttct tcttccaaaa gccggagctt ttcgaggagc gcctcgacga atttggaaaa
      181 ttccagctcg gcggtggctt ggggctcaag ctcgattgcg cggaatgtat ttccgaccgc
      241 atgggtgggc atggcagtcg aggctcctgt taaggatggt cctctcgctt tcgcctgtgt
      301 tactttcgca cagcgtatgg caaatacaat gccggtttga gcacagccgg acttgccgcc
      361 gccgcccgcg acggctgcct caggggtcaa aaagcgagtc tcggcagccg gaacggcgcc
      421 tggccgggtt tttaggtcaa tccgccgagc tacgtcggct ttcgctgcgg ccgcaacgtt
      481 ggtttgcgac caagttgatt gagcaagtgc cgagcacctt gccggtgtcg aggatttcga
      541 ggttgcggag tcctcggctg ggcggttgtg ccggcgtaag ctgctgtgta ggctggcgta
      601 atcgacccta gcgcaacttg cgattagagg cgtaagacaa tgggtatctt ccgaagaaac
      661 gtgccaatcc actacatccc ggtccatcca gacaacatca caaaaaagga ctggatcatt
      721 ctgctggagg ctggaacgat cgtcggccca tcagctgaaa agcttcgcgt tgcattagcc
      781 actttggaaa agaaaatcgt cacgaagtac aatattttcc ttaaacccga acacttcctc
      841 gttctggaag gatatgaccc aaggattcat gctttgtatt attgtcccca gctttacaag
      901 tatggcagcg ataagaagct ggtgcctttg gacggtgaag agatcggaag attggtcgca
      961 aaggcggtcc agaatggggt cgtggagaag cagccgtggt atccgccttc ggaaatgttg
     1021 ccacgcgagc ctatcgtcga tggaaagatg gacatcgatt ttgacagtat ggtcgttgac
     1081 gcccaaacat cattgtcgcc cactgctaga taaatacatt ccctaatcgg aacgcgttcg
     1141 cggggacgag cggacgtcgt gcggccggga tcacaccagc tccggccagg gcacgatggt
     1201 cgacttgacc gtcttcatcg ccatcgcgtc cttgaccgcg cgcgagacgc cgtcttcgtc
     1261 gaagggatag agcgtctgca tgtcgagcca aggatacttg tcgcgggcgc ggtcgagcat
     1321 gtcgacgccg agcggcaagt cgtttgcggt gaagccccac gaccccagca cgttgaggtc
     1381 cttggcgcag atgcggtgcc atgacgtggc gatcgagccg gcatcggtga actggcccat
     1441 ctccacataa gtgccgccgt cgcgcagcat ctcgatgccc tcgggaccgg cgctcggatg
     1501 gccggaacaa tccatcacga ggtcggcgcc aaagccgccg acgatgtcgc gcacgcgctt
     1561 gatgcgctga tctggctcct tgacctcctc gatgttcacc gtcgcctcgg cgccgaattt
     1621 gcgcgcgagc tcaagccgcg gcttctcggg cgcgccgacg cagatcaccc ggccggcgcc
     1681 catttcgcgc gcggcggcga tggcgagaat gccgatcggt cccgagccct ggatcaccac
     1741 cgtgtccccc cagctgaatc cacgcgctgc gaccgcgcgg ttgaacgcgc ggatgcagga
     1801 ggtcagcggc tccgacagcg cgccgagccg cagcggcatg tcgtcgggca gcttgtagac
     1861 cttcgtcccc ggcagcatgt cgaggtcgac atagacgtat tccgcgaagc ctccccacag
     1921 atgcggcggc ttgtcgaagc cgagatagcg cccgtaataa actggggtca ggcacttgtt
     1981 ggcctgctgc ggataatgga cgcagtaata gcactgcccg cacggcatca gcggcgggat
     2041 catcaccttc gagccgacgc cgagcttctt gcccatgaaa tcttcggtga actctcctcc
     2101 cttttcgacg atgatgccac cgagctcgtg gccgagggtg aaaggccacg gcagcggctt
     2161 cggccagtgg cctttgagga tgtgaaggtc ggtgccgcac accccgcagg cgccgatctg
     2221 aatcagcgcg gcgttcgcgg ggacttgcgg ccacggcacc ggccggatga ccggctcggc
     2281 gcctggaccg gcgaaggtgg cgacgcgaat ggtgctgttg cgcatggatt gttctcagct
     2341 cccatcttat cacgcatcac tgctgtcccg gcattgggcc agaatactcg cccgtagcgg
     2401 ccggtcgtca atcgtgaccg acgcatgtcc tgttgcaccc gtgtcgccac gccatgctag
     2461 agaaagtatc cgtgacgttc tcgctgggcg ggaaggtgag ccatggcggg ccatctcgat
     2521 ttctatttct tctacggcag catccactct tatctatcgg tcatgcgcat cggcccgctt
     2581 gcttccgcag cacgggtcga ggtgcgctgg cagccgttca atctgcgcga aattttgatc
     2641 gagcagaaca ataccgcatt cacgaagaac gacgtgaaga tgaattattt ctggcacgac
     2701 gtcgagcggc gcgcgcgccg ccacaacatc ccgttcgccg gccgggcgcc atacccggcc
     2761 gatcccgacc tcctcgcgct gcgggtgggc ctcatcgcgg cgcaagaaaa atggtgcgac
     2821 gactactcgc gcgccacgtt tcacgaatgg ttcatcgccc ggcgcgcgcc gggcgtcgac
     2881 gaccacatcg agcgcgttct tgcctcgctc gataaatcgc cggcgccgat catcgcccgc
     2941 gcgaagggcg cggagggcga acgtctgcta aaggcggcga cggatgccgc gcgcaagctc
     3001 ggcattttcg gcgcgccgac cttcgcgatc ggcgcggaga tcttttgggg cgacgatcgg
     3061 ctggaggagg ccctcgcctt cgcgggcagg acgctgattt gacttaaagg tcggcgtgct
     3121 gttcgcatcc aggagacgac gagcgtagcg ctgaattttt agatccagcg cgcatgcaat
     3181 aggagggaac tatggcgaac cccggcagca atcaggagac ggctgcgatc gaagccgcgt
     3241 accgagcgca agtggaactg ttgttcaaat ccttgatcac caacctgggc gaccaggtcg
     3301 tttcccatca gagcgatcag caaagtctcg acaagttcaa ggtgggactc aatatcgcga
     3361 agcgggcgaa gcaactggcg ttgaacgcgg tcgcccccgc gtcgcctgca agagccgctg
     3421 catcacgaag aaggaaacgc gaggaataac aatcgagagg tcggcgcgag ctataaagga
     3481 tgtcggcttc cgcagtgggc atctgacatt accaacgacg ccccttcaca aattgatttg
     3541 catgcagcgg accactgggt gcaggctgcc aatctcggcg cagcagcttt gacaggatga
     3601 gtcggctgcg aatgcctatt tgccgccggc tttcttaccc gcttgctgcc ctggcccaga
     3661 agggagcaaa caccatgagg ggaaagatgt tcacccgtcg ccgatttatg gcgacgtcag
     3721 cagctgcatc aacgacaatg attgcagctc cgttcgtcaa gaccgcgcat gcggctggca
     3781 agctctcgat cggcttttgg gaccattttg taccggacgc caacaagacg tccgatgcgt
     3841 taatccaaga gtgggcggcg aaggaaaagg tcgaggtcca gatcgaccgt tttaccggca
     3901 acaaggcgct aataaccatc gccgccgagg cgcaggccag gtcgggccac gacattttgt
     3961 acatgacgag ttggttgcca cacgcttatg cgagtagcct tgagccggtc gacgacgtca
     4021 tgatccagct cattaagcag aatggtccgg tgaacgacat cgtggcctat ctcggtcgag
     4081 ctgagagccg ttggcttgcg gtgccggcga cgatcgggaa ccagatccag ggaccgtgct
     4141 cgcgtatcga cctaatgaag caatacgccg gcatcgacgt tcaagccatg tacccggccg
     4201 gcgccccgcc gaaggctgat aactggacac tcgatacgtt cctcaaggcc gcggaggcgt
     4261 gccacaaggc cggattcccg tttgggatcg ggctcggaac gacggaggat tcggttgttg
     4321 ccgccggtgt catcttccag tgcttcggcg ccatgctcgt cgatgccaac ggga
//
LOCUS       DATLJT010001327        10626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3096491,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001327 DATLJT010000000
VERSION     DATLJT010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096491"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VM910_30475"
     CDS             complement(<1..1452)
                     /locus_tag="VM910_30475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI66868.1"
                     /translation="MNLLGKLTWSAIPFDQPIIMGASGGVVLVIMAILGWVTLKGYAP
                     YLWREWITSVDHKRIGVMYIVLGLIMLLRGFADAIMMRSQQAIAAGGAQGYLPPEHFD
                     QIFSAHGTIMIFFVAMPLVIGLMNFAVPLQLGIRDVAFPTLNSVSLWLTASGILLTNV
                     SLAVGEFAKTGWVAYPPLSELQFSPGVGVDYYLWSLQISGIGTLLTGINFVTTILKIR
                     APGMSYMRMPVFCWTALASNLMIVAVFPILTATFAMLLLDRYLGFHFFTVDAGGNAMM
                     YVNLFWMWGHPEVYILVLPAFGIFSEVAATFSGKPLFGYRSMVAATMAICVLSLTVWL
                     HHFFTMGASANVNAVFGIASMIIGVPTGVKIFNWMFTMYGGRIRFSVPVLWTIGFMVT
                     FVLGGLTGVLLAVPPVDWQVHNSAFLVAHFHHVIIPGVIFGVFAGYIYWFPKAFGFIL
                     DERWGRRAFWCWFIGFHVAFMPIYVVGLMGMTRR"
     gene            complement(1449..2327)
                     /gene="cyoA"
                     /locus_tag="VM910_30480"
     CDS             complement(1449..2327)
                     /gene="cyoA"
                     /locus_tag="VM910_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HVI66869.1"
                     /translation="MQHVALAILLIGTAALGGCSEGVLDPKGPVAAAERLVMFNSTGI
                     MLAIVIPTILATLGTAFWFRASNTRARYMPDFDYSGRLELLVWSIPLMTILLVGGVAW
                     LGSYDLNPPKAIASAVKPVRVQVVSLDWKWLFIYPDQGIAAVNQLTIPVGAPISFELT
                     SSGVMNSFFVPQLGGQIYTMSGMVTRLHLLADHAGTYRGMSANYSGAGFSDMHFSVDA
                     VPAEKFVQWVAATRTAGPVLDAQSYAELAKPSQAVTPFTYHAVTSGLFNAILSTAMQS
                     RDPLCLTPPASQRAPK"
     gene            complement(2329..3351)
                     /locus_tag="VM910_30485"
     CDS             complement(2329..3351)
                     /locus_tag="VM910_30485"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVI66870.1"
                     /translation="MKTTAIALSRLRLTSRPNLAARLPDFIALTKPRVMALAVFTALV
                     GLMVAPARLDPLLGFIAILAIAAGAGAAGVLNMWYDADIDAVMTRTAGRPIPREAISR
                     LEALAFGLVLACSAVAVLAFAANITAAALLAFAIFFYVVVYTIWLKRRTPQNIVIGGA
                     AGALPPVIGWAAASGEIALEPLVLFLIVFLWTPPHFWALSLNRADEYARAGVPMLPVV
                     AGRAETTRQILIYSILLLPISLLPWALGFAGAIYGATAVLCGAASIALALRLVGSNEA
                     DRRPAHRLFAFSILYLFLLFAALCAGNGGARWSLMVSARPTTAAVAPVDPTLYVTRGS
                     NLPEGA"
     gene            complement(3637..4890)
                     /gene="hemA"
                     /locus_tag="VM910_30490"
     CDS             complement(3637..4890)
                     /gene="hemA"
                     /locus_tag="VM910_30490"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HVI66871.1"
                     /translation="MNYRRNAKVDYDRFFADALGRLHSERRYRVFADLERIAGRFPHA
                     IWHSPGGPKQVVMWCSNDYLGMGQHPKVIGAMVETATRMGTGAGGTRNIAGNSHPVVE
                     LERELADLHRKEAALVFTSGYVSNETGISTLAKLIPNCLILSDAWNHNSMIEGVRQAH
                     TEKRIFGHNDMVHLEELLDNADPARPKLIVFESLYSMDGDVAPMRAICDLAERYGAMT
                     YVDEVHAVGMYGARGGGISERDGVAHRIDVIEGTLAKAFGGLGGYIAGSAALIDAVRS
                     YAPGFIFTTALPPSVAAAATAAIRHLKTSQWERERHQDSAARTKAVLAAARLPVTTSP
                     THIVPVYVGDPQLCKTASDLLLADHGIYVQPINYPTVPKGTERLRITPSPYHDDGLID
                     SLAEALVDVWGRLNLPFEERALAAE"
     gene            complement(4968..6287)
                     /locus_tag="VM910_30495"
     CDS             complement(4968..6287)
                     /locus_tag="VM910_30495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007801266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI66872.1"
                     /translation="MAETRPDEATAVVEAHPGERKRVVIIGGGFAGIAAAHALRHADA
                     EVLLIDRRNHHIFQPLLYQVATAVLSPAEIAAPIRQLEAKQRNVSVLLAEVTGVDVAS
                     RTVDAASASAGVRKIAFDYLVVATGMRPNYFGHDEFARHAPGLKNLNDAETIRAKILG
                     AFELAATTEDEGERARQMTFVLVGAGPSGVELAASLAQMVKVTLRNNFRRIDPSKSTI
                     ILLDAGNRVLPTFAEALSRRVTRHLTKLGVKVLTGVEVETVDEQGVVAGGKRIPSATV
                     LWTAGVAASPVPKMLGSKTDRAGRALVDPFLKVVDAPGVFVVGDAASVMQNGHPVPGV
                     AQAAIQQGRYVGRLIAKELKGRKVERPFSYFDKGNMAVVGMNYAVLERGWLRTSGFLT
                     WLVWAFVHILSLPQLQNRLRVQHQWLWSYFTGQRSSRLIPETHEPGH"
     gene            complement(6362..7915)
                     /locus_tag="VM910_30500"
     CDS             complement(6362..7915)
                     /locus_tag="VM910_30500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI66873.1"
                     /translation="MNIATTLRTEMPQPVLSIENVQPDLLTEAIQSRLGNPERSSDID
                     LHAATNQVLKDVRMSTADSGGEVTFYGRDPIIPSPFRFGSMAAIGLAAKAVAVAALWR
                     HRTGEGQDIGVDVRKALRRFAGFFDRKWETINGRPPLGWDATNPFQRLPLFRETADGR
                     YVVALNIYPKLSFGALNLLRCSPSVESVNNAILQWRADDLENAAAEAGLPIATVRTFE
                     EFRKEPQYTDVLSRIPLITVEKIGESEPMPFKRDGKSPLDGIRAFGMGHVIAGAGIGR
                     DLACYGADVLNIWRPENTEVEAFVWNVQIGMRSTILDNSKDDRAKFDHLLKDADVFFA
                     NKRPGYLERNGLDAEELCAKKPGLIHATVLLHGNKGPWKNRPGFDEIGGAVSGLFSIE
                     GTPTHPKSPPIIPICDNVVGWLGTIGILAALRRRAVEGGSYRVTVSLTRTLLWLYSLG
                     IFDKAYAQATAGSSDEHADIAPDLFIAETPLGTYQGMTDQVVLSRTPGSFRTVLAPRG
                     SSKPEWLAR"
     gene            complement(7967..8935)
                     /locus_tag="VM910_30505"
     CDS             complement(7967..8935)
                     /locus_tag="VM910_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVI66874.1"
                     /translation="MTDKPIRRIGIIGTGVIGASWTALYLAKGLHVVATDVAPNAEAA
                     LRKFVETAWPALKRLGLSPGASQSNLKFTAALAQAVSGTDLVQENGPERIDFKQKLYG
                     QLDELLPPDVIIASSSSGLTMSEIQKGAASHPERCVIAHPFNPPHLIPLTEIVGGAKT
                     SEETIRRAAEFYTSIGQKTVRLNKEMPGHVANRLQAALAREVYYLVAEGVVSAADVDT
                     ALSWGPGLRWGVMGNLMLNHLGGGPGGIEHFFQQFSGPMTAWWKTLGSPVLTPEVQKK
                     LIDSVHAEVGSRTIAELEAERDEVLLGLIELRKRSEAVAPRQVAAE"
     gene            complement(8957..9871)
                     /locus_tag="VM910_30510"
     CDS             complement(8957..9871)
                     /locus_tag="VM910_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HVI66875.1"
                     /translation="MTVLQATRTKTLSGATRSARLFLLELSGDRIHSMNPDGSDRKTI
                     VTGCRFPDGIAVDVAAGHVYWTNMGIPNLDDGSIERADLDGKNRKVIVPQGGTYTPKQ
                     LHLDKENGKLYWGDREGMRVMRCNLDGSQVETLVEAGRGDKDRQDQSRWCVGITIDPK
                     LGKIYWTQKGPDDAGLGRIFRANIEIPKGESPANRSDIEVLFDQLPEPIDLELDLKNR
                     VLYWTDRGDPPRGNTVNRAPIDKKAMPEILITHLMEGIGIALDVPGNRMFVTDFAGSV
                     YSADLDGKNERNFLYAQGNLTGIAYAEV"
     gene            complement(10261..>10626)
                     /locus_tag="VM910_30515"
     CDS             complement(10261..>10626)
                     /locus_tag="VM910_30515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66876.1"
                     /translation="RPMSINVEVIGGTLMVQHYVAEVHEPHHCRMVSLSDVQTPAGWT
                     KIQVIWDLSVTALDSASCRYTNRVVSYPTPAFLETLEVAGISFEDAVADRQAASDDHN
                     RRETALYAKSLERKALAHN"
BASE COUNT         2016 a         3277 c         3377 g         1956 t
ORIGIN      
        1 gcgccgcgtc atgcccatca ggccgaccac ataaatcggc atgaaagcga catggaagcc
       61 gatgaaccag caccagaacg cccgccggcc ccagcgctcg tcgagtatga agccgaatgc
      121 cttggggaac caatatatgt agccggcgaa gacgccgaat atgacgccgg ggatgatgac
      181 gtgatggaaa tgtgcgacca ggaacgcgct gttgtgcacc tgccaatcaa cggggggtac
      241 agccagcagc acgccggtca ggccgcccag aacgaacgtc accatgaagc cgatggtcca
      301 taggaccggc accgagaatc ggatgcgccc accgtacatg gtgaacatcc agttgaagat
      361 tttgacaccg gtcggcaccc cgatgatcat gctcgcgatc ccaaagacgg cattgacgtt
      421 tgcgctggcc cccatggtga agaagtggtg caaccaaacc gtgagggaga gaacgcagat
      481 cgccatggtc gcagcgacca tcgagcggta tccgaacagc ggcttgccag agaaagtcgc
      541 ggccacctcg gagaagatac cgaatgccgg caggacgagg atgtagactt cggggtgacc
      601 ccacatccag aacaggttca cgtacatcat cgcgttgccg ccagcgtcga ctgtaaagaa
      661 gtggaagccg aggtaacggt cgagcagcag catcgcgaac gtcgcggtga ggatgggaaa
      721 gactgcgaca atcatcaggt tcgatgcgag cgcggtccag cagaacaccg gcatgcgcat
      781 gtagctcatt cccggcgccc gtatcttcag gattgtggtg acgaaattta ttccggtcag
      841 cagcgtgccg atgccggaaa tctgcagcga ccaaaggtag tagtcgacgc caacgccagg
      901 cgagaactgc agctcgctca gcggcggata ggcgacccat ccggtcttgg cgaattcgcc
      961 gaccgccagc gagacgttcg tcaacaggat gcctgacgcg gtaagccaca ggctgaccga
     1021 gttcaatgtc gggaaggcca cgtcgcggat gccaagttgc agcgggaccg cgaagttcat
     1081 cagtccgatc acgagcggca ttgccacgaa gaaaatcatg atggtgccgt gcgccgagaa
     1141 aatctggtcg aaatgttcgg gcggcagata tccttgtgca ccgccagcgg cgatcgcctg
     1201 ctgcgagcgc atcatgatgg catcggcaaa gccgcgcagc agcatgatca atcccagcac
     1261 gatatacatg acgccgatgc gcttgtggtc gaccgacgtg atccactcgc gccagagata
     1321 aggtgcgtag cccttgagcg tgacccaccc aaggatcgcc atgatgacca ggactacgcc
     1381 tcccgaggca cccatgatga tgggctggtc gaacgggatc gcgctccacg tgagcttgcc
     1441 gaggagattc attttggcgc cctctgtgat gccgggggag tgagacacaa tggatctctg
     1501 gattgcattg cggtgctcaa gatcgcgttg aacaggcccg aggtcacggc atgataggtg
     1561 aacggcgtga ccgcctggct gggctttgcc agctcggcat agctttgcgc atcaagcacc
     1621 gggccggcgg tacgcgttgc agccacccac tgcacaaatt tttctgccgg gactgcatcg
     1681 acgctgaagt gcatgtcgga aaatccggcg ccgctgtaat ttgccgacat tccccgatag
     1741 gttccggcat gatcggccaa cagatggagg cgcgtcacca tccccgacat cgtgtagatc
     1801 tggccaccga gctgcggcac aaagaagctg ttcatgactc cggaagaggt cagctcgaag
     1861 ctgatcggcg cgcccacggg aatggtcagc tgattaacgg ccgcaatccc ctggtcggga
     1921 tagatgaaca gccatttcca atcgagcgac acgacttgaa ccctcacggg cttaactgca
     1981 gaggcgatcg ccttgggcgg attgaggtcg tatgagccca gccaggcgac gccgcccacg
     2041 agaaggatgg tcatgagcgg gatcgaccag acgagcagct ccaggcggcc ggaatagtcg
     2101 aaatccggca tgtagcgtgc gcgcgtgttc gaggcgcgaa accaaaaggc cgttccgagc
     2161 gtggcgagga tcgtcggaat aacgatcgcc aacatgattc cagtcgaatt gaacatgacg
     2221 agacgctcgg cggcggcgac aggtcctttc ggatcaagca cgccttcgga gcagccgccg
     2281 agggcggcgg tgccgattaa cagaatggcc agcgcaacat gctgcatgtc aggccccttc
     2341 aggtaggttg gaaccgcgcg tgacgtagag agtaggatct accggcgcga cggcagccgt
     2401 cgtgggacgc gcggagacca tcagcgacca gcgcgccccg ccattcccgg cgcagagcgc
     2461 tgcgaacagc agaaacaggt acaggatgga aaaagcgaac agcctgtgtg cagggcgtcg
     2521 atcggcttca ttgcttccga ccaacctcag cgcgagcgca atggacgctg ctccgcagag
     2581 gacggcagtc gcgccgtaga tcgcgccggc aaacccaagc gcccacggca gcagtgaaat
     2641 cggaagcagc agaatgctgt agatcaggat ttgtcgcgtg gtctctgctc tgccggccac
     2701 gaccggcagc atgggcaccc cggcacgcgc atattcgtcg gcgcgattga gcgacaacgc
     2761 ccagaaatga ggcggtgtcc acaggaacac gatcaggaac aagacgagcg gctcgagcgc
     2821 gatctcaccg ctcgccgccg cccaaccgat taccggcgga agtgcgccgg ctgcgccgcc
     2881 gatcacgatg ttctgtggcg tccttcgttt gagccatatc gtataaacga cgacgtagaa
     2941 gaagatggca aaagcgagca gcgccgcggc cgtgatattg gctgcgaaag caagaacagc
     3001 gacagcgctg caggcaagaa caaggccgaa ggccaacgct tccaggcgcg agatcgcctc
     3061 gcggggaatt ggacggccgg cggtgcgagt catcaccgca tcgatatcgg cgtcgtacca
     3121 catattcagc acgccggcgg ctccagcccc cgcggcgatt gccagaattg caatgaagcc
     3181 aaggagtgga tcgaggcgag ctggcgcgac catcaatccg acaagcgcgg tgaacactgc
     3241 aagcgccatc acccgcggct tcgtcaacgc gatgaaatcc ggcagtcgcg ccgccaaatt
     3301 aggccgtgaa gtgagccgta ggcgcgataa ggcaatcgcg gtggttttca tggtcgagtc
     3361 ctcaacaaag aaccgccttc gtgaagtttg cgaacttgcg ttcgccgcgg atcttgcgtt
     3421 tgtcgatcga tacaggcctg tgccgaagac cgtgagtaaa tgtcggcaaa tcctgccaaa
     3481 tctctccaaa ttgcgtcctt gaccggcaga acgatcgacg tcgcgggcgg cgcaccccaa
     3541 tacttgagtg tcagctcttc tcgcgcggca ctgctgaagt cgcgcaggag gtcgagcttg
     3601 cctgcgttga tctccgctcg tatctctgcc gccggactac tccgccgcca gcgcgcgttc
     3661 ctcgaacggc aggttgagcc tgccccagac gtcgaccagg gcctcagcaa gcgagtcgat
     3721 caggccgtcg tcgtgatagg ggctcggcgt gatccgcagt cgctcggtgc ccttcggcac
     3781 ggtcggatag ttgatcggct gaacgtagat gccgtgatcg gcaaggagca gatcgctcgc
     3841 cgtcttgcac aattgcggat cgccgacata gaccggcacg atgtgggtcg ggctcgtcgt
     3901 caccggcagc cgtgccgcgg cgagcaccgc cttggtgcgc gcggcgctat cctggtgccg
     3961 ctcgcgttcc cattgcgagg ttttcagatg ccggatcgcc gcggtcgccg cagcggcgac
     4021 cgacggcggc agcgccgtgg tgaagatgaa gcccggcgca taggagcgca ctgcgtcgat
     4081 cagggcagca gagcctgcga tataaccgcc gaggccgccg aacgccttgg cgagcgtgcc
     4141 ctcgatcacg tcgatgcggt gcgcgacgcc atcgcgttcg ctgatgccgc cgccgcgggc
     4201 gccgtacatg ccgaccgcgt gcacctcgtc cacgtaggtc atggcgccgt agcgctcggc
     4261 gaggtcgcag atcgctcgca tcggcgccac gtcaccgtcc atggagtaga gactctcgaa
     4321 cacgatgagc tttggccgcg ccggatcggc gttgtccaga agctcttcga gatgcaccat
     4381 gtcgttgtga ccaaaaatcc gcttctcggt gtgcgcctgg cgcacgccct cgatcatcga
     4441 attgtggttc caggcatccg acaggatcag gcagttcggg atcagcttgg cgagcgtcga
     4501 gatgccggtc tcgttcgaca catagccgga ggtgaagacc agcgccgcct ccttgcggtg
     4561 caggtcggcg agctcgcgct cgagctcgac caccggatga ctgttgccgg cgatgttgcg
     4621 ggtaccgccg gcgccggtgc ccatccgcgt cgccgtttcg accatggcgc caatcacctt
     4681 cggatgctgg cccatgccga gataatcgtt ggagcaccac atcacgacct gcttcggccc
     4741 gcccggcgaa tgccagatgg cgtgcgggaa gcggccggcg atgcgctcga gatcggcgaa
     4801 cacgcggtag cggcgctcgc tatgcagccg gccgagcgca tcggcaaaga agcggtcgta
     4861 atcgactttg gcattccgac gataattcat gagggtccgc gtccgacggc ggggccgctc
     4921 ttggccctgt gccagcttcc ctggttgggg ttcccttgct cggtgactca gtgcccaggc
     4981 tcgtgggttt ccgggatcaa gcgcgagctg cgctggccgg tgaagtacga ccacagccac
     5041 tggtgctgca cgcgcagccg gttctgcagc tgcggcagtg acaggatgtg aacgaacgcc
     5101 cacaccagcc aggtcaggaa gccgctggtg cgcaaccagc cccgctccag caccgcatag
     5161 ttcatgccga cgacggccat attgcccttg tcaaaatagc tgaacggacg ctcgaccttc
     5221 cgccccttca gctctttcgc gatcagccgc ccaacgtacc gcccttgctg aatcgctgcc
     5281 tgcgccacgc cgggcaccgg atgcccattt tgcatgaccg acgccgcgtc gccgacgacg
     5341 aacactcccg gtgcgtccac gaccttcagg aacgggtcga caagcgcccg tcccgcgcgg
     5401 tcggtcttgc taccgagcat ctttggaacc ggcgatgccg cgacacccgc ggtccacagc
     5461 acggtggcgc ttggaatcct ctttccgccg gcgacaacgc cttgctcgtc gacggtctcg
     5521 acctcgactc cggtcaggac cttgacgccg agcttggtga ggtgccgggt caccctgcgg
     5581 gacagcgcct cggcgaacgt cggcaggact cggttgcctg cgtcaagcag aataatggtg
     5641 ctcttcgacg gatcgatgcg gcggaagttg ttccgcagcg tgaccttcac catttgggcc
     5701 agggacgccg cgagctcgac gccgctaggg cctgcgccga ccagcacgaa cgtcatctgt
     5761 cgcgcgcgtt cgccctcgtc ctcggtcgtt gccgccagtt cgaacgcgcc caggattttg
     5821 gcccggatcg tctccgcgtc gttgaggttc ttgagcccgg gagcatggcg cgcgaactcg
     5881 tcgtgcccga aatagttggg gcgcatgccg gtcgcgacca ccagatagtc gaacgcaatc
     5941 ttgcggacgc ccgcgctcgc ggaagccgcg tcaaccgtgc gggaagcaac gtcgacgccg
     6001 gtgacctctg ccaggagtac gctgacgttc cgctgcttcg cctcgagctg acggatcggc
     6061 gcagcgatct cggcaggaga gaggaccgct gtcgccacct gatagagtag cggctgaaag
     6121 atgtggtggt tgcgccggtc gatcagtaag acctcagcat cggcgtgccg cagcgcgtgc
     6181 gccgctgcga tcccggcgaa gccgccgccg atgatgacga cgcgcttgcg ctctccggga
     6241 tgcgcctcga cgacagcggt ggcctcgtca ggccgggtct cggccaacat tgcgctcatg
     6301 gatcgcctcg ttcgtgctct tatgagatgg cttcagcaac tcgcgttcgg gaacagcgct
     6361 ctcaacgggc cagccactcg ggtttgctcg agcctcgcgg cgcaagcacc gttctgaagg
     6421 agccgggagt ccgcgacagg actacctggt cggtcatccc ttgatacgtg cccagcggcg
     6481 tctccgcgat gaacagatcg ggagcgatgt cggcgtgttc gtcggaagat ccggccgtcg
     6541 cctgggcata ggccttgtcg aaaatgccga gcgaatagag ccacagcaag gttcgggtca
     6601 gggaaacggt gacccgatag ctgccgcctt ccaccgcacg ccgccgcagc gccgcgagaa
     6661 tgccgattgt gccgagccaa cccacgacat tgtcgcaaat ggggatgatc ggcggcgatt
     6721 tggggtgcgt cggcgtcccc tcgatagaga acagtcccga tacggcccca ccgatctcat
     6781 cgaatccagg acgattcttc caagggccct tgttaccgtg gagaaggacg gttgcatgga
     6841 tcagccccgg cttcttggcg cagagctcct cggcatcaag cccgttccgc tccaaatatc
     6901 ctgggcgctt gttggcgaag aatacgtcgg catccttcaa caggtgatcg aatttcgcgc
     6961 gatcgtcctt ggaattgtcc aggattgtcg aacgcatccc aatctgtacg ttccagacaa
     7021 acgcctcaac ctcggtattt tcgggacgcc agatgttgag gacatctgcc ccgtagcagg
     7081 cgagatctct gccgatgccg gctccggcga tcacatgccc cattccgaat gcgcgaatgc
     7141 catccagcgg gctcttccca tctcttttga aaggcatcgg ctcgctttcg ccgatcttct
     7201 ccaccgtgat caacgggatc cgggaaagca cgtcggtgta ttggggctcc ttccggaatt
     7261 cctcgaaggt gcgcaccgtg gcgatgggaa gcccggcctc ggccgccgcg ttttccaggt
     7321 cgtcggcgcg ccactgcagg atggcattgt tcacggattc gacgcttggg ctgcacctca
     7381 ggaggttgag agcgccgaac gatagcttgg ggtagatgtt cagcgctacg acgtaccgcc
     7441 cgtcggccgt ctcccgaaac agcggcaaac gttggaacgg gttcgtggcg tcccagccaa
     7501 gcgggggacg tccattgatc gtttcccatt tccggtcgaa gaagccggcg aagcgccgta
     7561 acgccttgcg cacatccacc ccgatatcct gtccctcacc ggtccggtgt cgccaaagcg
     7621 ctgcgaccgc aacggccttg gcggccaggc cgatcgcagc catgctgcca aaccggaagg
     7681 ggctcggaat gatcggatcg cggccgtaga aggtgacttc gccgccgctg tcggcggttg
     7741 acatccgaac gtctttcaga acctgattgg ttgcggcgtg caggtcgata tccgaactgc
     7801 gttccggatt cccgagcctg gactgaattg cctcggtgag cagatccggt tggacatttt
     7861 cgatggacag aacaggttga ggcatttctg tccgcagcgt ggtcgcaata ttcatttgca
     7921 aactcctttt cggttagggg ggcgttggct gtccgcagcc gcaccgttac tcagctgcga
     7981 cttggcgcgg ggcgaccgcc tccgaacgct tgcgcagctc gatcaggccg agcagaacct
     8041 cgtcccgctc cgcttccagc tccgcgatgg tgcgcgatcc gacctccgca tggacgctgt
     8101 cgatgagctt cttctgcacc tcgggcgtca gtaccggcga acccagcgtc ttccaccagg
     8161 ccgtcatcgg accgctgaac tgctggaaga agtgctcgat gccgcccgga ccgccgccga
     8221 ggtgattgag catcaagttg cccatcactc cccagcgcag gccggggccc caggagaggg
     8281 cggtgtcgac gtcggcggcg ctcaccacgc cctcggcgac aaggtagtag acctcgcgcg
     8341 ccaatgcggc ctggagccgg tttgcgacgt ggccgggcat ctccttgttg aggcgcaccg
     8401 tcttctggcc gatcgacgta taaaactccg ccgcgcgccg gatcgtttct tctgaggtct
     8461 ttgccccgcc gacgatctcg gtgagcggga tcaagtgcgg cggattgaac ggatgggcga
     8521 tgacgcagcg ctctggatgc gacgcggcgc ccttctggat ttcgctcatg gtgagccccg
     8581 acgagctcga cgcgatgatc acgtcgggcg gcagcagctc gtcgagctgt ccatagagct
     8641 tctgcttgaa gtcgatccgc tcgggtccgt tctcctggac gagatcggtg ccggagaccg
     8701 cctgcgcgag cgcggccgtg aacttcaggt tcgactgcga ggctccgggc gagaggccca
     8761 atcgcttgag cgccggccag gcggtctcga cgaacttccg gagcgccgcc tctgcgttcg
     8821 gcgccacgtc ggtcgcgacg acgtgcagtc ccttggcgag gtacagcgcg gtccagctcg
     8881 cgccgatcac gccggtgcca atgatgccaa tgcggcgaat gggcttgtcg gtcatgtcag
     8941 ttctccaacg tgttgctcag acctcggcat aggcgatgcc ggtgaggttg ccttgcgcgt
     9001 agaggaagtt gcgctcgttc ttgccatcga gatcggcgga atagaccgat ccggcgaagt
     9061 cggtgacgaa catccggttg ccgggcacgt cgagggcgat gccgatgcct tccatcaggt
     9121 gggtgatcag gatctcaggc atcgcctttt tgtcgatcgg cgcgcgattg acggtgttgc
     9181 cgcgcggagg atcgccgcgg tcggtccagt agaggacgcg gttcttgaga tcgagctcga
     9241 ggtcgatcgg ttccggcagc tgatcgaaca gcacctcgat atcggagcgg ttcgccgggc
     9301 tctcgccctt cgggatctcg atgttggcgc ggaagatgcg ccccaggccg gcgtcgtccg
     9361 gacctttttg cgtccagtag attttcccaa gcttcgggtc gatggtgatt ccaacgcacc
     9421 accgactttg atcttgtcgg tccttgtcgc cacggccggc ctcgaccagc gtttcgacct
     9481 gcgagccgtc gagattgcag cgcatcacgc gcatgccctc gcggtcgccc cagtagagct
     9541 tgccgttctc cttatcgagg tggagctgtt tcggcgtgta tgtcccgcct tgcggtacga
     9601 tgaccttgcg gttcttgcca tcgaggtcgg cgcgttcgat cgagccgtca tcgaggttgg
     9661 gaatgcccat gttggtccaa tagacgtgac ccgccgctac atccacggcg atgccgtcgg
     9721 ggaagcggca accggtgacg atggtcttgc ggtcggaccc gtcgggattc atcgagtgaa
     9781 tgcggtcgcc gctcagttcg agaaggaaca atcgtgcaga gcgtgtcgcg cccgataaag
     9841 tcttggttcg cgtggcctga agtacggtca tggttttgtc ctttcgtgtg cctctacttg
     9901 tcccgcatgc gccgtcgacg ccagtgaagc cgaacaaatc tcgccaaatc tttgctaacg
     9961 cgacgacgga tcgcggctaa cgcgacgacg gaccgcgtac gtctgatcag gcaagatcaa
    10021 tggacggctt gaggggctgt tgatcagcat cgagcaagac cacaccagcc gtcctcgatg
    10081 ctcttgggct ggctgtgggc ggaggcgaac gagtcccgcg gcgtcctgct attagtctgt
    10141 taggtgaaac tgacttcgct gatgtcgatg gataccatca gcatgtccaa aatcggcagg
    10201 cgtccagggc cgcgcgtgta ggcgcgacgt ttggcatgcg tcggagcaga atcaggaatc
    10261 ctaattgtgg gccagggcct ttcgttcgag actttttgca tagagagctg tctcacggcg
    10321 gttgtggtcg tcactggcgg cttggcggtc agccacggcg tcttcgaacg agatcccggc
    10381 gacctcgagc gtctccagga atgccggtgt cgggtagctg accacccggt tggtgtatcg
    10441 gcagctggct gagtccagtg cggtgacgct gaggtcccaa attacctgga tcttcgtcca
    10501 gccggccggt gtctgcacgt cgctgagcga caccatccgg cagtggtggg gttcatgtac
    10561 ctcggccacg tagtgctgca ccatgagggt gccgccgatc acctccacgt tgatcgacat
    10621 cggccg
//
LOCUS       DATLJT010001328         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_569777,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001328 DATLJT010000000
VERSION     DATLJT010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569777"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VM910_30520"
     CDS             complement(<1..354)
                     /locus_tag="VM910_30520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66877.1"
                     /translation="MASCIGRRKFLATLGGAAAAWPLAAGAQQPAIPVIGFLDAATAA
                     DTVYRVSAFREGLKEAGFIDGHNVTIVFRWAENKLDRLPALAADLVQRQVAVIVGPNI
                     VMSAARAATSTIPIVF"
     gene            complement(618..1514)
                     /locus_tag="VM910_30525"
     CDS             complement(618..1514)
                     /locus_tag="VM910_30525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66878.1"
                     /translation="MKLPRRTFLHLAAGAAALPAVSCFAWAQAYPTRPVRLIEGFGAG
                     GAPDIIARLIGQSLSEQLGQSFVIENRSGASGNIATDAVARASPDGYTLLLITSTTNA
                     INTTLYDKLSFDFIRDIVPIASIARAPLVMEVHPSVPARTVAEFIAYAKAIPGKVNMA
                     SAGTLTQMAAEMFKMMAGLDLFHVPYRGAQVFPALLAGEAQVYFGPLLSSIGYVRAGN
                     LRALAVTTTTRSDVLPDVPVLSDTLPDYEASAWFGIGAPKSTPAELIERLNKEVNISI
                     ADPKFKARLANLGGTVLGRLAG"
     gene            1874..2113
                     /locus_tag="VM910_30530"
     CDS             1874..2113
                     /locus_tag="VM910_30530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66879.1"
                     /translation="MIEPLCRLCLEAGRVTAASVADHHPPHGGDFNAFRLGPLRSLCV
                     ACHAQLDGNNVPRFPVREDGTPSGPNHPWNRGAQT"
     gene            2110..2271
                     /locus_tag="VM910_30535"
     CDS             2110..2271
                     /locus_tag="VM910_30535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66880.1"
                     /translation="MIDLKFDTSGAVQRLEQMRRDLGEFASRTMSEELTQWEVADLNR
                     GRSTGGIGQ"
     gene            2603..>2681
                     /locus_tag="VM910_30540"
     CDS             2603..>2681
                     /locus_tag="VM910_30540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HVI66881.1"
                     /translation="MNVITLASRKGGAGKSTLTAHLAACA"
BASE COUNT          559 a          796 c          809 g          517 t
ORIGIN      
        1 gaacacgatc ggtatggtcg aggtggcagc ccgagccgcg ctcatgacga tatttggacc
       61 gacgatcacg gccacctggc gctgcaccag atccgccgcg agtgctggca accgatcgag
      121 cttgttctcc gcccagcgaa atacgatcgt gacgttgtgg ccgtcgatga agcctgcttc
      181 cttcaggccc tcgcggaatg ccgatacccg ataaactgta tcggccgcgg tcgcggcatc
      241 aaggaaaccg atcaccggta tcgccggctg ctgcgcgccc gccgcgagcg gccatgcagc
      301 cgcagcgccg ccgagcgtgg ctaagaattt tcgtcgtccg atgcagcttg ccatgtgacc
      361 ctccgatcgg gggtcattca tgcaatggag ggatgatacc acgcttccat cgcgcggtct
      421 gtgactgagg ccggtaggcc aatcgctggg gtcaaaagcg cacggccgag ggccgccctc
      481 cggtttttcg caccccccgc agtgcgcgga acggaggtta tggaacatcg cgagggatca
      541 cctacattcg cttgatgttg gccgccacaa tcaccttgcc ccatttcttg gtctcgtcgg
      601 agatgagttt gccgaaatca gccggcgagc cttccaagca ccgtgccgcc cagattggca
      661 agccgtgctt tgaattttgg atctgcaata ctgatattga cttccttgtt cagtctttcg
      721 atgagctctg cgggcgtgct cttcggagca ccaatgccga accatgcact tgcctcgtag
      781 tccggcaacg tgtcgctcag aaccggaacg tccggcagca cgtcagaccg cgtcgtggtg
      841 gttacggcca aggcgcgcag attaccggcc ctgacatagc caatcgagga gagcagcgga
      901 ccaaaataga cctgggcctc gccggcaagc agcgcgggaa acacctgcgc gccgcgataa
      961 ggcacgtgaa acaggtcgag gcccgccatc atcttgaaca tttcggcggc catctgggtc
     1021 aaagttcccg ctgatgccat gttgaccttg ccgggaatgg ccttggcata agcgatgaat
     1081 tccgcaacgg tcctggccgg aaccgacggg tggacctcca tgaccaaggg cgcgcgagcg
     1141 atgctcgcga tcggcacgat atcgcggatg aagtcgaaac tgagcttgtc gtagagcgtc
     1201 gtgttgatag cgttcgtcgt cgacgtgatg agaaggagcg tgtagccgtc cggggatgcc
     1261 ctggccacag cgtcggtggc aatgttgccg ctggcaccac tgcggttctc gatgacgaat
     1321 gactgcccca gttgctccga cagcgattga ccgatcaatc gcgcgatgat gtcgggtgct
     1381 ccgccagcgc caaagccttc gatcaagcgc actggtcgcg tcggataagc ttgcgcccaa
     1441 gcgaagcacg acacggccgg cagcgcggca gcgcctgctg ccagatgcaa gaatgtacgg
     1501 cggggaagtt tcattttgtt ctacactatt gcaacagcca caacgacttg cattgtctac
     1561 ctgcccttaa cggcgctacg tgtgcgttgg ggaatttccc cgactgtcta acctagtcct
     1621 agaaattccg ggacgcgtcc acacgaacgc gcgtgtgtct ggcatgccca ttggcccttg
     1681 gctggctcag gcatggacga gagaattctc ggtgtgtcgg cagcccaaac gcgtttggtt
     1741 tcaacatacc gtactctcgc ggcttggaaa atgtaagaaa acggggaaga ccttgccctc
     1801 gatgccgcac cgtcgggaag ttcatcgttg gtacggcact gctggctggc agcgccgccg
     1861 agctcatcag ctcatgatcg agccattgtg taggctctgc ctcgaagccg gacgcgtcac
     1921 ggcggcgagc gtcgccgatc accatccgcc gcatggtggc gacttcaatg cgttcaggct
     1981 gggcccgctg cgcagcttgt gcgtcgcgtg ccacgcccag ctcgacggca acaatgtgcc
     2041 acgctttccc gtgcgtgagg atggcacgcc gagcggtccg aaccatccct ggaacagggg
     2101 cgcgcagaca tgatcgacct gaagttcgat acgagtggtg ccgtgcagcg gctggaacag
     2161 atgcgaaggg accttggcga gtttgcctcc aggacgatga gcgaggagtt gacgcagtgg
     2221 gaagtcgccg acctcaatcg cggcagaagc acgggaggca tcgggcagtg agacggcgct
     2281 gccaagaacc acgcagaacg cgcacagcac gatcatgtcg cgcccaggaa attgatcgaa
     2341 gttcaagcgc tcgcaccgac tgaaaacaca atagaattcg gcaagacctc gatggaagac
     2401 agttccaaaa aacaagaaaa acaaactttt catgattatc ccttcgcagg ctgggacacc
     2461 gcttttaaca ccggctgcgc catttaatta acgctagtta ctttaattgt tcgaagcctc
     2521 gcggtttgcc gtaggccgca gaagcgttag actgcagttg ctgaaatctc gtgatcacct
     2581 acgtgggtgg tcgtaagcga taatgaatgt catcaccctc gcgtcgcgaa agggcggggc
     2641 cggcaagagc acgctgaccg cgcatctcgc cgcctgcgcg c
//
LOCUS       DATLJT010001329         2387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4243894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001329 DATLJT010000000
VERSION     DATLJT010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4243894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..2156
                     /locus_tag="VM910_30545"
     CDS             372..2156
                     /locus_tag="VM910_30545"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HVI66882.1"
                     /translation="MFLRVVRAAGGKGVRHEYVRVVEAFRQNGKTRHRTVLNLGRKDL
                     LAAHLDLDKLMRLLHGEAVPDRAIREENVGAVAAWDWGPMLVARLLWCELGLEATLDR
                     LATPTRRDATTLSDRALVLVVNRLTVPGSEHGLARWLETDFVCDRQGRRWLPAWRDDG
                     ERVASRTPRVRVALRQLKGWYRTLDQLLERKAEIEYALFLTLRDLFSLKVDLVFYDLT
                     STYFEGRGPPGIGANGHSRDGKPRNPQVLVGLVLVDGWPIAHHVFAGNRRDAKTVPDV
                     LRDLEERFGLKRVVFVGDRGMVTSHNLDDLRAHGHGYIVGRNRRRSGEVFDYIQSATG
                     PWIQCPVGMTAREKASSPKTLVQEVLSNQPGVRVFVAHSEERQTFERAHRIKAMERVR
                     ARLEKLQSRIAKGQLKAAEKVGAAAARVLARNHGHRYYGWSYEEGVFRFFEHPVHFAR
                     EQAYEGKYVIQTEEPDLSAVDAVRLYKELSEVERCFANLKDVIDLRPVYHQTEDRVEA
                     HIFVAALAFLIHRAIEKKLKAARLDLSATEALSILKSIRVVDIDLGNGTTKRSVTAGT
                     QRAAAVLRAIGITDRDPPIPPTPGETVM"
BASE COUNT          456 a          741 c          736 g          454 t
ORIGIN      
        1 caaaagcaaa ggcattaaca ttcaaagtca caaatgaact atcttacaaa caatccacgc
       61 ctatcgccga cggctctgac caactttctc gggtgccatc atcttgctgt gcttgagcgg
      121 atggaagcgc ataagcttgc aaagagaccc ttctttgatg acccgatgct cgaaatcttg
      181 cgggaccgcg gactagccca tgtttccctt ttccgcccgg aggctgatcg ctaagtagct
      241 gataatattg atgtcggctt tggatttctc caaaatgtag ggacagagat agcgttatta
      301 tttcgcactt tggtattgct gttcgctgat tctcatgcga ttcccccagg gacacggttt
      361 cggaggaggc catgttcctg cgggtggtcc gcgccgctgg cggcaaaggc gtcaggcacg
      421 aatatgtgcg ggtcgtcgaa gcctttcggc agaacggcaa gacgcgacat cgcaccgtcc
      481 tcaatctcgg ccgcaaggac ctgctggcgg cccatctcga tctcgacaag ctgatgcgcc
      541 tattgcacgg cgaggcggtg cctgaccgcg ccatccgcga ggaaaatgtt ggcgcggtcg
      601 cggcgtggga ttggggaccg atgctggtgg cgcgcctctt gtggtgcgag ctcggcctcg
      661 aagcgacgct cgacaggctt gccacgccga cccgtcgcga cgccacgaca ctgagcgatc
      721 gcgctctggt gctggtcgtc aatcggctga ccgtgccggg cagcgagcat ggcctggcgc
      781 gctggctgga gactgatttt gtgtgcgacc gccagggccg gcgatggctc cccgcatggc
      841 gcgacgacgg cgagcgcgtc gccagtcgaa cgccgcgggt gcgggtggcg ctgcgtcagc
      901 tcaaggggtg gtaccgtacg ctcgatcagt tgctggagcg caaagccgag atcgagtacg
      961 cgctcttcct aacgctgcgc gacctgttct cgctcaaggt cgatctggtg ttctacgacc
     1021 tgacctcgac ctatttcgag ggccgggggc ctcccgggat cggcgccaat ggccacagcc
     1081 gggatggcaa gccgcgcaat ccgcaggtgc tggtcggcct ggttctggtc gacggttggc
     1141 cgatcgccca tcacgtgttc gcgggcaacc ggcgcgatgc caagacggtc ccggacgtgc
     1201 tgcgtgactt ggaggaacgc ttcgggctca agcgtgtcgt gttcgtcggc gaccgcggca
     1261 tggtgacgag ccacaacctc gatgacctgc gcgcgcacgg ccatggctac atcgtcggcc
     1321 gcaaccgccg gcgcagcggt gaagtgttcg actatatcca aagcgcaacc ggcccctgga
     1381 tccaatgccc ggttggtatg acggcgcgcg agaaagcgtc atcgcccaag acgctggttc
     1441 aggaggtttt gtccaaccag ccgggggtgc gggtgttcgt cgcccactcc gaagaacgcc
     1501 agactttcga gcgcgcccac cgtatcaagg cgatggagcg ggtgcgggcc aggctcgaaa
     1561 agctacaaag ccgcattgcc aagggacagc tcaaggccgc cgagaaggtc ggcgctgctg
     1621 cggcgcgcgt gctcgcccgc aatcatggcc accgctatta cggctggtcc tacgaggagg
     1681 gcgtgtttcg cttcttcgag catccggtcc actttgcgcg cgagcaggcc tacgagggca
     1741 aatacgtcat ccagaccgaa gagcccgatc tgtccgccgt cgatgcggtg cgcctctaca
     1801 aggagctttc cgaagttgaa cgctgctttg ccaatcttaa agacgtcatc gatctgcggc
     1861 cggtctatca tcaaaccgag gatcgcgtcg aggcgcatat ctttgtcgcc gcgctggcct
     1921 tcctcatcca tcgcgccatc gagaagaagc tcaaagccgc gcgcctcgat ctttccgcca
     1981 ccgaagcgtt gagcattctc aaatccatcc gcgtggtcga tatcgatctc ggcaacggca
     2041 cgacaaaacg ctccgtcacc gccggcacac agcgcgccgc agccgtgctg cgcgccatcg
     2101 gcatcaccga ccgcgacccg cctatcccgc ccaccccagg cgaaaccgtg atgtagtgac
     2161 aaatcaaaaa tcgacctcag caaaatcaat gcgttacagg ggcaaaaggg aaacatgggc
     2221 tagcggcctc tcggcggcgc ggcccccgtc gtcgtggtgc cggggatcgg gatgatctca
     2281 gcgatcgacc ggtccgtcgg gtccaccatc agcacacgat ccggcagctt gacgaattca
     2341 taattcttgg tctccggcac cagttgagtg actgagttcg gcatggc
//
LOCUS       DATLJT010001330         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1718731,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001330 DATLJT010000000
VERSION     DATLJT010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718731"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VM910_30550"
     CDS             complement(<1..793)
                     /locus_tag="VM910_30550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HVI66883.1"
                     /translation="MWSRPLALALLLLPTPALADVKEKVAALAPSGVVLVVDLEGNEL
                     VAQNADEPFVPASVTKIVTAWLALEVLGGDYRFETRFYLDNKRVLYVRGGGDPFLISE
                     ELAPLATDLVAVVGKAPIDSIVLDASYYPSNLRIPGIEDTNNAYDALNSALAVNFNTI
                     YAVRRGNKVSSAEKQTPITPLAIDQFRERGPRGTGRISLSQDPTVSLQYAGELIAGFI
                     KRAGGSVKGKISTGTVPEGLKPVYVHRQSRPVSKILAELLRASNNY"
     gene            991..1248
                     /locus_tag="VM910_30555"
     CDS             991..1248
                     /locus_tag="VM910_30555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66884.1"
                     /translation="MDPDKRSAWLQKLDAECPYQVVLPRRQLTDDSEIVNFLLSYAGK
                     FDMYVEDECAAYVRYCFKDPMDAAIFRSRFERKSEPFRLAG"
     gene            1347..1472
                     /locus_tag="VM910_30560"
     CDS             1347..1472
                     /locus_tag="VM910_30560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66885.1"
                     /translation="MLAALAITLKVKIDTPQGPQPLARAIVTTHLQRLQGELALP"
     gene            complement(1487..1855)
                     /locus_tag="VM910_30565"
     CDS             complement(1487..1855)
                     /locus_tag="VM910_30565"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVI66886.1"
                     /translation="MVFAEEAGSRNDALQPEAASSISSGLSIVGKIIGHGALTIFGHV
                     EGEVRASTIVIAEGAHIEGKVSAEELTIGGCVTGTIHANRVQLNSTAVVAGDIFHRTL
                     VIEGSDASMKSARAIVRWNV"
BASE COUNT          467 a          651 c          656 g          446 t
ORIGIN      
        1 tgtagttgtt cgaggcgcgg agcaactcgg cgaggatctt cgaaacagga cgcgactgtc
       61 gatgaacgta gaccggcttt agtccctcgg gcaccgttcc ggtcgagatc tttcctttca
      121 cgctgccgcc ggcccgcttg atgaacccgg cgatcagctc gccggcatat tgcaggctca
      181 ccgtggggtc ctggctcagg ctgatgcggc cggttcctct agggcctcgc tcccggaact
      241 ggtcaattgc gagcggggtg atcggggtct gcttctcggc ggagctcacc ttgttgcccc
      301 tgcgcacggc gtagatggtg ttgaaattca ccgccagcgc ggagttcagc gcatcgtaag
      361 cattgttggt gtcctcgatg cccgggatcc gcagatttga agggtagtag ctcgcgtcaa
      421 gcacgatgct gtcgatcggc gccttgccga ccactgcgac cagatcggtc gcgagcggcg
      481 ctagctcctc tgagatcagg aaaggatcgc cacctccgcg cacgtagagc acacgcttgt
      541 tatcgagata gaagcgggtc tcgaagcggt agtcgccgcc cagcacctcc agcgccagcc
      601 aagccgtcac gatcttggta actgaggccg gaacgaaggg ttcatcagcg ttctgggcca
      661 ccaactcgtt gccttcgagg tccaccacta gcaccacccc cgacggagcg agggccgcga
      721 ctttctcttt gacgtcagcc agggccgggg tgggtagcag aagcaaagcg agggcgagcg
      781 gacgagacca catggcgagt ccacactcag ttaatgtgcg ctttcggggg taaagcggac
      841 attggtcgaa ctcagcgaaa cgtccgctag tcgatcccgc cttaactcag tgtttaccat
      901 gatggcgatt ctcattcacg ggcgcttgac cgaaatagaa cagacaaaga acaatttatc
      961 tctgagtcgt tgacatagag gaggttaaac atggacccgg acaaacgaag tgcttggctg
     1021 caaaagctcg atgcggagtg cccgtaccaa gtcgtgctcc cgcgacgaca gcttactgac
     1081 gacagcgaga ttgtgaactt cctgctgtca tacgcgggca agttcgacat gtacgtcgaa
     1141 gatgaatgtg ctgcgtacgt acggtactgt tttaaggatc ccatggacgc cgcgattttc
     1201 cgatcgcggt tcgaacggaa aagcgagccg tttaggctcg cgggttgagc gccgcctacc
     1261 gggcaccgct ctgtcggcag ctgcttcatc gggcccgaag ggccaagggc aattgaggat
     1321 gctcaagatc agtgtggtgg gttggcgtgc tggcggctct cgcgatcacg ctcaaggtca
     1381 agatcgacac gccgcagggg ccgcagccac tcgctcgggc gatcgtgacc acgcacctgc
     1441 aaaggctaca gggcgagctg gcgctgccct aagcggcgtc aggtccctat acgttccagc
     1501 gcacgattgc gcgagccgat ttcatgctgg catcactccc ctcgatcacc agtgttcgat
     1561 gaaagatatc gccggcaacg acagcggtac tgtttagttg gacgcggttg gcatggatgg
     1621 tgcccgtgac gcagccgccg atcgtcagtt cctctgcgct gaccttgccc tctatgtgtg
     1681 ccccttcggc gatcacgatg gtcgaggcgc gtacctctcc ctcgacgtga ccaaaaatag
     1741 tcagggcccc atggccgata atcttgccga cgatcgaaag gcccgaacta atcgaggagg
     1801 ccgcttccgg ctgcaacgca tcgttacgtg acccagcttc ttcggcaaac accatgtccc
     1861 cctccctaaa acggcactta ctgtcgggca ttgcggtgaa cgtagcaagc tgcccgagct
     1921 attgagccgc caccggacat ggggagtgcg ataaggcgcg gcggatcgcc ttacttgctg
     1981 gcccaaacga ccatttaatt ggccccaatt atcaagccac cggcagaatc agttcggata
     2041 attcggcggg atttttcata acgccgatgc cgaacgaata ccggaaaaaa aacaaggacc
     2101 ctacgtttga ggatttgaat ccgcgcacgc tatttcgcga gtttgcggcc gaatgcatgg
     2161 aattagctca gacgaccccg acgcctgaga aacgcctcct atatctgaaa atggcgcgcc
//
LOCUS       DATLJT010001331         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4015865,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001331 DATLJT010000000
VERSION     DATLJT010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4015865"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VM910_30570"
     CDS             complement(<1..457)
                     /locus_tag="VM910_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HVI66887.1"
                     /translation="MKIAVINLGVIVSGDWHSPLAAGDAILIDNGRISDVGGASEETV
                     SDADVVIDADGMTAIPGLIDSHVHITFGDYTPRQHTVGYLGSYLHGGVTTAISASEVH
                     VPGRPRDVEGVKALAIAAQRCFADFRPGGMRVIAGSVILEPGLTRTDFDE"
     gene            complement(454..1944)
                     /locus_tag="VM910_30575"
     CDS             complement(454..1944)
                     /locus_tag="VM910_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HVI66888.1"
                     /translation="MTTCKTGADHINSLKDGRTVYLDGKLVGDVTEHPAFRNTVRSAA
                     LLYDFQSRPENIELMTFVPNGANRRVNRAWQIPRNYQELVYRRKALQAWAALSYGFMG
                     RSPDHLASSLVGQRIGLDVFERHGPARAQAFRNYFDEATRNDYFLTYVIINPQAERAK
                     DWGEQVEELVARIVDEDSGGITIRGAKMLGTSSIMANEVFVANLQPLKPGEEALAFSC
                     ALPMGTKGLRVLSRKSYEAAAVSVFDNPISSRFDENDALIYFDDVKVPWERVFVHRDT
                     DMCRAQFHDTAGHVYQNYQSQIRLSVKIKFLAGLAHRIAEAIGTTNMPSVREQLGFLA
                     AQASMVNAMLAGMEAEGSMRGKYYVPNRHYMYSAQVLTQDLYPRVVNTLRDLSGGALI
                     MLPSSVFDFEDEKLRRIINMTQRAAKMGPEEKVKFLKAAWDAIGSEFGSRHTQYEMFY
                     AGARFVTTGHSYRTYDWDGATGLVDALLHSYGLDDELRKRRQDRLE"
     gene            complement(1983..2555)
                     /locus_tag="VM910_30580"
     CDS             complement(1983..2555)
                     /locus_tag="VM910_30580"
                     /inference="COORDINATES: protein motif:HMM:NF018399.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HVI66889.1"
                     /translation="MLIKRTLCFVDEVTSDAGEIIEPALRKVAVAGVIDNPFAGRFES
                     DLSPLVKASEQIGREICKLAIALLAPHKPQSYGKGAIVGVNGEQEHCNAMLTTVFGNV
                     MREAAGGGKAWISSITKRASAGVSLDIPLAHKDALYVRSHYDGITITLADAPLPNEIA
                     IICAYANRGRPHARLGGLAAKDIKGEDGLT"
     gene            2667..>2838
                     /locus_tag="VM910_30585"
     CDS             2667..>2838
                     /locus_tag="VM910_30585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI66890.1"
                     /translation="MKPAKFDYVVPRTLEEATAALATANGDGKILAGGQSLVPMLNFR
                     LLKPALLVDINRI"
BASE COUNT          519 a          860 c          881 g          578 t
ORIGIN      
        1 gctcgtcgaa atcggtgcga gtgagtcccg gctcgagaat gaccgaaccg gcgatcacac
       61 gcatgccgcc cggccggaaa tcggcgaagc agcgctgcgc ggcaatggca agcgccttca
      121 cgccttcgac atcgcgtggc cggccgggca cgtgcacttc ggatgctgaa atggccgtgg
      181 tgacgccgcc gtgcagataa ctcccgagat aaccaaccgt atgctggcgc ggcgtgtagt
      241 cgccgaaggt aatgtgcaca tgtgaatcaa tcaggcctgg gatcgcggtc atcccgtcgg
      301 catcgattac cacgtcggcg tcgctgacgg tctcctcgct cgccccgcca acgtcgctga
      361 tcctaccatt gtcgatcaga attgcgtcgc cggcagcgag cgggctgtgc cagtcgcctg
      421 atacgatcac acccagattg atgacggcga tcttcattcg agtcgatcct gccgccgctt
      481 gcggagctca tcgtcgaggc cgtaggagtg cagcagcgca tcgaccagac cggtggctcc
      541 gtcccagtcg taagtccggt agctgtgccc ggtggtgacg aagcgcgcgc ccgcgtagaa
      601 catctcgtac tgggtgtggc gggagccgaa ctcggagccg atcgcgtccc acgctgcctt
      661 gagaaacttg accttctcct ccggccccat ctttgccgcg cgttgcgtca tgttgatgat
      721 cctccgcagc ttctcgtcct cgaagtcgaa aacggaggag ggaagcatga tcagcgcgcc
      781 gcccgaaaga tcgcgcagcg tattgacgac acgcgggtag agatcctgcg taagcacttg
      841 cgccgaatac atgtaatgcc tgttaggcac gtaatactta ccgcgcatcg agccttctgc
      901 ctccatgccg gcgagcatgg cgttcaccat gctggcctgc gccgcgagga agcctagctg
      961 ctcgcgcacc gacggcatat tggtggtgcc gattgcttcg gcgatccggt gggcgagccc
     1021 ggccaggaac ttgatcttca cgctcagacg gatttgcgat tgatagtttt gatagacgtg
     1081 cccggcggta tcgtggaatt gggcgcggca catgtcggtg tcgcgatgga cgaacacgcg
     1141 ttcccacgga accttgacgt cgtcgaagta gatcagcgcg tcgttttcgt cgaagcgcga
     1201 ggagatcgga ttgtcgaaca ccgaaaccgc tgccgcctcg taggatttgc gggaaagaac
     1261 gcgcaggccc ttagtgccca ttggcagtgc gcaagagaag gctagcgcct cttcgccggg
     1321 cttgagtggc tgcaagttgg caacgaaaac ctcgtttgcc atgatcgagc ttgtaccaag
     1381 catcttggcg ccgcggatgg ttataccgcc ggagtcctcg tcgacgatac gggcaaccaa
     1441 ctcctcgacc tgttcgcccc aatccttcgc gcgttcagcc tgcgggttaa tgatcacgta
     1501 agtgaggaaa taatcgttgc gggttgcttc atcgaaatag ttgcggaagg cctgcgcgcg
     1561 ggcagggccg tggcgctcga atacgtcgag tccgatccgc tgcccgacca gcgacgacgc
     1621 aagatggtcg ggcgagcgtc ccatgaagcc gtaggacagc gccgcccagg cctgtagcgc
     1681 tttgcgccga tagaccagct cctggtaatt gcgcgggatt tgccaggcgc ggttcacgcg
     1741 gcgattggcg ccgttcggca cgaaggtcat cagctcgatg ttttccggcc gtgactgaaa
     1801 atcgtagagt agggcggccg atctgaccgt gttgcggaat gccggatgct cggtgacgtc
     1861 tccaaccagc ttgccgtcga gatagaccgt gcgaccgtcc ttgagcgaat tgatgtgatc
     1921 cgcgccggtt ttgcaggtgg tcatggtagg ctccgtcgca aggttgctcc tttgatggca
     1981 gcttacgtca gcccgtcctc gcctttgatg tctttcgcgg ctagcccgcc gaggcgcgca
     2041 tggggccgcc cgcgattggc gtaggcgcag atgatcgcga tttcattcgg caacggcgca
     2101 tccgccagtg tgatcgtgat gccgtcgtaa tgcgagcgta catagagtgc gtctttgtgt
     2161 gcgagcggga tatcgaggct gacgccagcg ctcgcgcgct tggttatcga cgagatccac
     2221 gcctttcccc cgcccgccgc ctcgcgcatc acgttgccga acacggtcgt gagcatggca
     2281 ttgcagtgct cctgttcgcc gttgacgccg acgatggcgc ccttcccata gctttgcggc
     2341 ttgtgcggtg cgagaagcgc gatcgccagc ttgcagattt cccggccgat ttgttcactc
     2401 gcctttacaa gcggagaaag gtcgctctca aaacgtccgg cgaacggatt gtcgatcacg
     2461 ccggcgacgg cgaccttgcg cagcgcaggc tcgatgatct cgccggcatc gctggtcact
     2521 tcatcgacga agcacagcgt gcgcttgatc aacatgtttc cccgctacgc agcacatttg
     2581 tcggcttgca gccgcgcctt tttcgtcagc attggttagt ctagtaatgc atgtcaacag
     2641 ctgtcgccgt ggcagccgga gccgggatga agcctgccaa gttcgattac gtcgttccca
     2701 ggacgctcga ggaagcaacc gcggcgctgg caacggccaa cggggacggc aaaatcctgg
     2761 ccggcgggca aagcctcgtt cccatgctca atttccggct gctgaaaccg gcgctcctgg
     2821 tcgacatcaa tcggatcc
//
LOCUS       DATLJT010001332         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1259075,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001332 DATLJT010000000
VERSION     DATLJT010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259075"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VM910_30590"
     CDS             <1..189
                     /locus_tag="VM910_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HVI66891.1"
                     /translation="GQIVGRTAYLQPAHVMASPDIIGEILPVRLESLERYSLLGELAA
                     PAPSRTDRSTASSITIGA"
     gene            194..1246
                     /locus_tag="VM910_30595"
     CDS             194..1246
                     /locus_tag="VM910_30595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVI66892.1"
                     /translation="MPKSASDSPSLAPSRKFDRDMQVPPETQVVIDFDDNRAASALVG
                     PYGQNLAQIERRLGVVVDSRGNHITIAGSRDGCDAARRVLEALYAQAVQGHDLTQGDV
                     EGAIRAVIAQGSLFEYDAKTARSTFETINLRKRPVRARTAAQDSYIRALKRYELVFGV
                     GPAGTGKTWLAVAHAAQLFERKEVDRIILSRPAVEAGERLGFLPGDLREKVDPYLRPI
                     YDALYDLMDARIVERALQTGEIEIAPLAFMRGRTLTNAAIILDEAQNTTSMQMKMFLT
                     RLGENSRMIITGDPSQVDLPFGQTSGLAEAARLLDGVDGIAQVHFTSEDVIRHELVAR
                     IVAAYEGAPRKPATSK"
     gene            1297..1806
                     /gene="ybeY"
                     /locus_tag="VM910_30600"
     CDS             1297..1806
                     /gene="ybeY"
                     /locus_tag="VM910_30600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVI66893.1"
                     /translation="MLHLTLPMTEVLVVADCWQAAPEAESVIQRAIATAAQAVNADVA
                     GAELAIMLTNDSGICDLNRKFRGVDKPTNVLSFPPPQPAGPREAHDTPRVLGDIAIAY
                     ETMHREANEEGKPFAHHLSHLAVHGFLHLIGYDHETEDDAEAMEALEVEILARLGIPD
                     PYGDRERMD"
     gene            1808..>2139
                     /locus_tag="VM910_30605"
     CDS             1808..>2139
                     /locus_tag="VM910_30605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt efflux protein"
                     /protein_id="HVI66894.1"
                     /translation="MADSEPTHDNPRNMRNLPAIVPPGEVARPASENWLLRAVRTLFG
                     WKAGSVRDDLQVVLDASQPDEVGFTAVERTMLRNILSLHERRLADVMVHRADIIAVKR
                     DIPLGELM"
BASE COUNT          405 a          720 c          653 g          361 t
ORIGIN      
        1 ggccagatcg tgggacggac cgcttatctg cagccggcgc atgtgatggc ctctcccgac
       61 atcatcggcg aaatcctgcc ggtccgcctg gaaagcctcg aacgctacag cctgctcggc
      121 gaactcgccg cgcccgcccc atcgcgcacc gaccgctcga ccgcttcgtc catcaccatt
      181 ggagcctgaa cccttgccca aaagcgcatc ggattcgcct tcgctcgctc ccagccgcaa
      241 gttcgaccgc gacatgcaag tcccgcctga gacgcaggtc gtcatcgact tcgacgacaa
      301 tcgcgcggcc tccgcattgg tcggccccta tggacagaac ctcgcacaga tcgagcggcg
      361 cctcggcgta gtcgtcgatt cgcgtgggaa tcacattacg attgccggct ctcgcgacgg
      421 ctgcgacgcc gcacgacggg tgctggaggc gctgtatgca caggcggtcc agggtcacga
      481 tctaacgcag ggcgacgtcg agggcgcaat ccgcgcagtg atcgcgcagg gctcgctgtt
      541 cgaatatgat gcgaaaacgg ccaggtccac cttcgagacc atcaatctgc gcaagcgccc
      601 ggtccgggcg cgcaccgcgg ctcaggactc ctacattcgc gccctcaaac gctacgaact
      661 cgtgttcggc gtcggtcccg ccggcaccgg caagacctgg ctcgccgtgg cgcatgcggc
      721 gcaattgttc gagcgcaagg aggtcgatcg catcatcctg tcgcgaccgg cggtcgaggc
      781 cggcgagcgc cttggttttc tgcccggcga tttgcgcgaa aaggtagatc cctatctcag
      841 gccgatctat gacgcgctgt acgacctgat ggatgcgcgg atcgtcgagc gtgcgctgca
      901 gaccggcgaa atcgagatcg cgccgctcgc tttcatgcgc ggacgcacgc tgaccaatgc
      961 cgccatcatc ctggacgagg cgcaaaacac cacgtccatg caaatgaaga tgtttttgac
     1021 ccggctcggc gagaacagcc gcatgatcat caccggcgac ccatcccagg tcgacctgcc
     1081 gttcggccag acctccggtc ttgccgaagc tgcgaggctg ctcgatggcg tggacggcat
     1141 cgcgcaggtc cactttacgt ccgaagacgt gatccgccat gaactggtgg cgcgcattgt
     1201 cgcggcttac gaaggggcac cacggaagcc cgcgaccagc aagtaactag gagtggccgc
     1261 gccacggcgc gcctggatcg aattcctgaa acaacgatgc tgcatctcac ccttcccatg
     1321 actgaggtcc tcgtggtcgc cgattgctgg caggccgcgc cggaggccga atccgtcatc
     1381 cagcgcgcga tcgcaacagc cgcccaggcg gtgaacgccg acgtcgcagg ggccgaactt
     1441 gcgatcatgc tgaccaacga ttcggggatc tgcgacctca acaggaaatt ccgcggcgtc
     1501 gacaagccaa ccaacgtgct gtcctttccg ccacctcagc cggcggggcc gcgcgaggcg
     1561 catgatacac cgcgcgtgct tggcgacatc gcgatcgcct atgagaccat gcaccgggaa
     1621 gccaatgagg aaggcaagcc gtttgcgcat cacctgagcc atcttgcggt ccacggtttc
     1681 ctgcatttga tcggctacga tcacgaaacc gaagacgacg ccgaggcgat ggaagcactc
     1741 gaagtagaga ttctggcgcg actcgggatt cccgatccct atggggatcg ggagcggatg
     1801 gattgagatg gcggactccg aaccgaccca cgacaatccg cgcaacatgc gcaacctgcc
     1861 cgcgatcgtg ccgccgggcg aggtcgcacg tcccgcctct gaaaactggc tgctccgcgc
     1921 ggtccgcacc ctgttcggct ggaaggccgg atcggttcgc gacgatctgc aggtcgtgct
     1981 tgacgcctcg cagcccgacg aagtcggctt taccgccgtc gagcgcacca tgctgcgcaa
     2041 catcctgtcc ctgcacgagc ggcgccttgc ggacgtgatg gtgcatcgcg ccgacatcat
     2101 cgcggtcaag cgcgacattc cgctcggcga attgatgga
//
LOCUS       DATLJT010001333         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1027498,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001333 DATLJT010000000
VERSION     DATLJT010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027498"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1683
                     /locus_tag="VM910_30610"
     CDS             1..1683
                     /locus_tag="VM910_30610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI66895.1"
                     /translation="MLLPAGVTVQDEDGNLLLVNDVAAAQLGVSQSEAAEPSPQLIQR
                     RSQGLELLLAGRAAVTEESTGDGQLKQVLLTSHRPVRIDGRNLLLSCSADISEQKAFE
                     DQLFRSAYYDELTDLPSRRVIEHRVNGLLQRSAANEKFALAFLDIDNFKHINDYYGHA
                     VGDALLTGLAKRLGRELRETDMLSRISGDEFLLLLCPVQGSHEVIEFIEQTLQRLRAP
                     FFIDGSEIFASTSIGISLYPDHGTNYEVLRQNADLAMYRVKNDGKGAAAFFDAGMEQE
                     ALVRTRIEQSLRLAILEKRFCCAFQAKVDIRTQEIKGIEALVRLRDDESVIQAPGSFI
                     DLAVELGLIDDLTHLVLAEIVKSIDLINETFGSDTTISINVAAKQAGNLEFMRSFAGA
                     LEATGYPNRFMIEVTEDAFVTRTHFQDEIVPILRKIGVGISIDDFGIGYSSLSALADL
                     TADEIKIDRSFITDIHERPRSQGILRAIESLSEALGMTVVAEGIESFEELAYLQAATK
                     IRYAQGFYFSRPVFLEELKPAFPLASEARVNLASRPVQETRPAYSRGRAYGR"
     gene            1839..>3041
                     /locus_tag="VM910_30615"
     CDS             1839..>3041
                     /locus_tag="VM910_30615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HVI66896.1"
                     /translation="MISSIQDRSSKAASAPWRRLAFGQRRDPIFWLIASGCVLVAAIC
                     VGTMIMIGEFRERALKNGERELENTVMLLTRHFDQQFEDSEAIAAEVIGQMKTAGIDS
                     SEIFKRRMSTLEMHQVLHSKVSALSYIGDIAMFDADGTLLNWSRSFPPPPVSIAERPY
                     FKIFKTDPQSEPIVAEPARSYVDGNWTTIVAHRLNASDGTLLGVVTRRIDPANYERFF
                     ASLALGEGAPISMFHSDGTMLARYPHVDALIGRKFKDAPLVNRALAAGGQQTLRVKSP
                     VDGLDRLGAAAQLGDFPIVVVATNTVAAALTDWRAQTRFLVTAAALSASLIACILFLI
                     IRQIIQHNRDAQQRLEAEKHRLDTALNNMTQGLVQYDASARIVTFNQRYVDMYKLSTD
                     VVKPGLHYY"
BASE COUNT          612 a          961 c          897 g          571 t
ORIGIN      
        1 atgctcctgc cggccggtgt cacggtgcag gacgaagatg gcaacctgtt gctggtgaac
       61 gatgtcgcgg cggcccagct cggggtgtcc caaagcgagg ctgccgagcc ttcgccgcaa
      121 ctgatccagc gccgcagcca aggcctggag ctgctcctcg ccggacgcgc cgccgtcacc
      181 gaggaatcaa ccggcgacgg ccagctcaaa caggtcctgc tcacgagcca ccgccctgtt
      241 cgcatcgacg gccgcaatct gctgctgtcg tgctcggccg atatcagcga acagaaggcg
      301 ttcgaggacc agctcttccg ctcggcctat tatgacgaac tgacggacct tccgtcgcgg
      361 cgggtgatcg agcatcgcgt caacggcctg ttgcagcgta gtgcggcgaa cgagaaattc
      421 gcgctcgcct ttctcgacat cgacaatttc aagcatatca acgactatta cggccacgca
      481 gtcggcgatg cgctgctgac cggccttgcc aagaggctgg ggcgcgagtt gcgcgaaacc
      541 gacatgctgt cgcggatttc cggcgatgaa tttctgctgc tgctctgccc ggttcagggc
      601 agtcacgaag tgatcgaatt catcgagcag accttgcagc ggcttcgagc gccgttcttc
      661 atcgacggct cggaaatctt cgcctcgacc tcgatcggga tcagcctgta tccggatcac
      721 ggcacaaatt acgaggtgct gcgccagaac gccgaccttg cgatgtaccg cgtcaagaac
      781 gacggcaagg gcgcggccgc cttcttcgat gccggcatgg agcaggaagc gctcgtgcgc
      841 acaagaatcg agcagtcgtt gcggcttgct atcctggaaa agcggttctg ctgcgccttc
      901 caggccaagg tcgacatcag gacgcaggag atcaagggca tcgaggccct ggtacgactg
      961 cgcgacgacg aaagcgtgat ccaggcgccc ggctccttca tcgatcttgc agtcgagctc
     1021 ggcctgatcg acgacctcac gcatctggtg ctggccgaga tcgtcaaatc gatcgacctg
     1081 atcaacgaga cgttcggaag cgacaccacg atcagcatca atgtcgccgc caagcaggcc
     1141 ggcaacctcg agttcatgcg ctccttcgcc ggggcgctgg aggcgaccgg ctatcccaac
     1201 cgtttcatga tcgaggtcac cgaggacgct ttcgtcacca gaacccattt ccaggatgaa
     1261 atagtgccaa tcctcaggaa gatcggagtg ggaatctcga tcgatgattt cggcatcggc
     1321 tattcgtccc tgtcggcgct ggccgacctc accgccgacg agatcaagat cgaccgctcc
     1381 ttcatcaccg acattcatga gcggccgcgc agccagggca tcctgcgcgc gatcgaatcc
     1441 ttgagcgaag cactcggcat gacggtggtc gccgagggca tcgaatcctt cgaggagctt
     1501 gcttacttgc aggccgcaac caaaatccgc tacgcacagg gcttctactt ttcccggccc
     1561 gtcttcctgg aagagctgaa gccggccttc ccgctcgcca gcgaagcgcg tgtcaacctt
     1621 gcaagccggc cggtgcagga aacccggccc gcctattcgc gcgggcgcgc ttacggccgt
     1681 tagagcatga tccggaaaag tggtcgcctt tcgggcgtta agtctgagtt aactcaaact
     1741 ctccgcacaa aataccgatt aaatcgaaag taaccggatt tcctaacggc ttatgccatt
     1801 tcggcatcgt attcatcccc ttcgagggga aatacgcgtt gatttccagc attcaggacc
     1861 gatcgtcaaa agccgccagc gccccgtggc ggcggctcgc attcgggcag cgccgcgacc
     1921 ccatcttctg gttgatcgcc agcggctgcg tcctggttgc ggcgatctgc gtcggtacaa
     1981 tgatcatgat cggcgaattc cgcgagcgcg cgctgaagaa cggcgagcgc gagctggaaa
     2041 acaccgtaat gctcctgacg cggcacttcg accagcagtt cgaggattcg gaggcgatcg
     2101 cggccgaagt catcggacaa atgaagaccg cgggcatcga ttcatctgaa atattcaagc
     2161 ggcggatgtc cacgctggag atgcatcagg tgctgcattc gaaggtcagt gcgctgtcct
     2221 acatcgggga tatcgcgatg ttcgatgccg acgggacact gctcaactgg tcgcgaagct
     2281 tccccccgcc gcccgtcagc atcgccgagc ggccctactt caagatattc aagacagacc
     2341 cgcaatcgga gccgatcgtc gccgaaccgg cgcgcagcta cgtcgacggc aattggacga
     2401 ccatcgtggc ccaccggctg aacgcgtcag acgggacgct gctcggcgtg gtaacgcggc
     2461 gcatcgatcc tgccaattac gagcggttct tcgcttcgct tgcacttggc gaaggcgccc
     2521 cgatttcgat gtttcacagc gacggaacca tgctcgctcg ctatccgcac gtcgatgctc
     2581 tgataggacg gaaattcaag gatgcgccgc tggtaaacag ggcgctggcc gccggaggtc
     2641 agcagacgct gcgcgtgaaa agccccgtcg acggactcga ccggctcggc gcggcagcgc
     2701 agctcggcga tttcccgatc gtggtggttg cgaccaacac ggttgccgct gcgttgaccg
     2761 actggcgcgc gcaaacccgg ttcctggtga ctgccgcggc gctgtccgcc tcgctgattg
     2821 catgcatcct gtttttgatc atccgccaga tcattcagca taaccgcgac gcacagcagc
     2881 ggctggaagc cgagaagcac cggctcgaca ccgccctcaa caacatgacg cagggtctcg
     2941 tgcagtacga cgcgtcggcg cgcatcgtca ccttcaacca gcgttacgtc gacatgtaca
     3001 agctgtcgac tgacgtggtg aaacccggct tgcattatta c
//
LOCUS       DATLJT010001334         4418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1719272,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001334 DATLJT010000000
VERSION     DATLJT010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719272"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VM910_30620"
     CDS             <1..179
                     /locus_tag="VM910_30620"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI66897.1"
                     /translation="GLANYYRNNVIGGAGAFVAVAENFNSFGNAITKKLIAEIAQAEP
                     QQQENSQKRPNLRN"
     gene            568..1005
                     /locus_tag="VM910_30625"
     CDS             568..1005
                     /locus_tag="VM910_30625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI66898.1"
                     /translation="MTAIGGSSLCGGVKFEITGRLSSPLNCHCSQCRKQHGAPFRSRV
                     RVQVEDFRWLQGEHLIKYYESAGGYLRGFCRECGSPIINRTGPNWKRAAQFPCAKSQY
                     GIPLAILDDPPVRPACHVFVGSKAPWFEITDDLPQYAEYAPPG"
     gene            1291..1524
                     /locus_tag="VM910_30630"
     CDS             1291..1524
                     /locus_tag="VM910_30630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66899.1"
                     /translation="MTPNILENISTWLVDWSLITIMSNTPPPPDPNDENDEDDEDDED
                     DKNDEDDKDDDEEDENDDDERQDEPAVVREPDE"
     gene            1799..2572
                     /locus_tag="VM910_30635"
     CDS             1799..2572
                     /locus_tag="VM910_30635"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVI66900.1"
                     /translation="MLDTTAPTPLQQVDNLLVKRDDLFKIAGVCGGKARTCWAMGQGA
                     PGLVTAVNRSSPQGDVVAHVAKALGVPCRVHVPTGKLGPELVAAQAAGAVVIRHSPGH
                     SSVINARARDDACERGWRVIPFGMECIEAVEQTAAQARSTVAQMYNQQAGALRVVVPV
                     GSGMSLAGILHGLAQAAFTVPVVGVVVGADPVKRLDKWAPKHWRNLCQLVQAGIDFHK
                     PAPVVRLGDMELDPIFEAKCLPFLQPGDLFWVVGIRQGP"
     gene            2704..2967
                     /locus_tag="VM910_30640"
     CDS             2704..2967
                     /locus_tag="VM910_30640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66901.1"
                     /translation="MSRKLVFVEQFDDLTFSVPLFVLFGWRMSQLSYVFSSHEETSEM
                     PLSSSTVLRRFRPHRTKKQRGRSGERCAAFIGKRAHFLIVTRS"
     gene            3320..4114
                     /locus_tag="VM910_30645"
     CDS             3320..4114
                     /locus_tag="VM910_30645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HVI66902.1"
                     /translation="MASKITIRSFIAWSILPLLLMIAAGAWFALGTGFRSGHRSAILS
                     ASMPKDEFEQRIQAYLLDHPEVIVESLNRMEERQRASAETEVQAIIQSRADELFRDVD
                     GPIAGNPNGNATLVEFFDYNCPYCRQMVPVMTQAESADPQLRIVYKEFPILGPNSKLA
                     AKAALAAHKQGKYLAFHKALFQVRGTVDPSKVAEVAAAVGLDMDRLKADVEDPAIAAL
                     IEKNLALAQALHIDGTPGFVAGRHVLRGAVDLKALQGFLQEARSNP"
BASE COUNT          945 a         1279 c         1368 g          826 t
ORIGIN      
        1 gcgggcttgc aaactactat cgcaacaacg tcatcggcgg cgccggcgcg ttcgtcgcgg
       61 tcgcggagaa cttcaattcc ttcggcaacg ccatcaccaa gaagctgatc gcggagatcg
      121 cgcaagccga gccgcaacag caggagaatt ctcaaaaacg tcccaatttg aggaattgag
      181 accgggccgc aagagaacga gcaattacgg gcgcgtgcaa gcgtcaaagt cgctgcgtcg
      241 cggagaactt caattccttc ggcaacgcca tcaccaagaa gctgatcggg ccacacgacc
      301 gctgactgat tcctatcgat tattctattc ttactctcgt cactcgtggg gatttggaga
      361 tcagatgcaa ttcgatcagt tgaaatggcg tgaatttatt gcagcacttg gtggcgctgc
      421 cgcgtggccg ctcgctgcgc aagcacaaca agcagaccga cgtgcccagc actccgccag
      481 ggcttgaagg aaactggcca catccaggga tgaacaggga ggagcttgcc ttcctaggat
      541 ggcgaagttc acgaaaagga gatcggcatg acagcgattg gcggaagctc cctttgcggc
      601 ggcgtaaaat tcgaaatcac cgggcggcta tcaagcccgc tgaactgcca ttgttcgcaa
      661 tgtcgcaagc agcatggcgc gccgttccgc agccgcgttc gggtgcaggt cgaagacttc
      721 cggtggctgc agggcgagca cctgatcaag tattacgaga gcgcgggcgg ctacctgcgc
      781 gggttctgcc gtgaatgcgg gtccccgatc atcaaccgga cgggaccgaa ctggaagcgc
      841 gccgcccagt tcccctgcgc aaagtctcaa tacggcatcc cgttggccat tctggacgat
      901 ccaccggtgc ggccggcatg ccacgtcttc gtcggcagca aggcgccgtg gttcgagatc
      961 accgacgatc tgccgcaata tgcggaatac gcgccgccgg gctaaaacct cggcagatcg
     1021 ggatgcggca agcggccggc atggacatgc aaccggccga ggtcggcgca gcggtctgag
     1081 tcgcgtccct caggaacctt ctttgcttgt tccaggttat ctcggcaagt ggcgctctcc
     1141 gggaagtaaa atcagcccac ggaggcaatc gagagtagcc gcggggagcc ccggatcata
     1201 ccaccggggc tctcccaatt tcctagctcc gccccctcgg gcggttccat aacccgtcgc
     1261 gaatgtcgcg acacccaaac aggaggcgac atgaccccga atatcctcga gaacatttcc
     1321 acttggttgg tcgactggag cctcatcacc atcatgagca acacgccgcc gccgccagac
     1381 cccaacgatg agaacgacga ggatgacgag gacgacgagg acgacaagaa cgacgaggac
     1441 gacaaggacg acgacgagga ggacgagaac gacgacgacg agaggcagga cgagccggcg
     1501 gtcgtcagag aacccgacga atagccgccg cgcgcgcctc aacacagcgc gcgcctcggc
     1561 ttcccgcgcc tctccgcgaa tggaacgacg acgacttcgg cgccatagtc ggcggcatca
     1621 tgaaggtcac tgcgttaccg gacgatgccc tgagggtgta ggatggtcag gtctcacgcc
     1681 ggcgccgggg cggccatgac cgggaatggg ttgcccccat gcttggtggg atgaaggtag
     1741 acgcgcaatt atcgggcgct aaaactgacg gccaccgcgc ggaatggatg attcaaacat
     1801 gctcgatacg acggcgccga cgcctttgca gcaggtagac aatctcctgg tcaagcgcga
     1861 cgatttgttc aagatcgcag gtgtgtgcgg cggcaaagcg cgcacctgct gggccatggg
     1921 ccaaggcgct ccgggcctgg tgacggctgt caaccgctcc agcccgcagg gcgacgtcgt
     1981 agctcacgtc gccaaggcgc tcggcgtgcc atgccgggtt cacgtgccca caggcaaact
     2041 cgggccggaa ctggtggccg cccaggctgc cggcgcagtc gtaattcggc attcgcccgg
     2101 gcatagcagt gtgatcaacg cccgggcccg cgacgatgca tgcgagcgcg gttggcgggt
     2161 gattccgttc ggcatggaat gcattgaggc agtcgagcaa acggccgccc aggcgcggtc
     2221 gacggttgct cagatgtaca atcagcaagc gggggcactc cgggtggtcg tgccagtggg
     2281 ttccggcatg tcgttagcgg gaattctgca cgggctcgcg caggcggcct tcactgtgcc
     2341 cgttgtcggc gtcgtggtgg gcgccgaccc tgtcaagcgc ctggataagt gggcgcctaa
     2401 gcattggcgc aacctgtgcc aactggtcca agccggcatc gactttcaca aaccggcgcc
     2461 cgtcgttcgg ttgggcgaca tggaactgga cccgatcttt gaggccaagt gcctgccgtt
     2521 ccttcagccc ggcgatctct tctgggtagt cggcattcgc caggggccgt aagagaatat
     2581 gcgtttgcta ctctgccaat ttcgaaaaaa tacggatcga attttgggct atagggatgc
     2641 tctgttgaga ttgcgacaat taaaacggtg agaccatgat cggaattggc gcgtgatgtg
     2701 acaatgtcgc gtaagctggt gtttgtagag caatttgacg atctcacctt ttcagtgccg
     2761 ctgttcgtgc tctttggttg gcggatgagc cagctcagct atgtgttctc gtctcatgag
     2821 gaaacgagcg agatgccgct cagttccagc accgtgctgc ggcgcttcag gccacatcgg
     2881 acgaagaaac aacgcggtcg atctggtgag agatgcgctg ctttcatagg caagcgagcg
     2941 cattttctca ttgttactcg atcttgagaa aaaggggtat gctttctgtt cggaccacca
     3001 atgcagacat tcggcggacg caggcccgtg ttgcattggg cagagatagc cctcgggagt
     3061 tgaggatgac cccgagccga gtgcgccagg aatgcggttt cagatggtcc gcagacaatc
     3121 tcgatcgttg gttgactgat cccggcaagt tcttggccgg aacgagaatg ccggtccacg
     3181 tgctggatgc ttcggctcgg cgggacatca ttgcctatct gcagaaagaa agccgccaca
     3241 cgggcgacgc gtccgagccg aaatcgatcg cacaacgctc atccgaacga tctcggaagc
     3301 agtgagagag ggctggggaa tggcatctaa aatcacgatt cgatctttca tcgcatggag
     3361 cattttgccg ctgctcctga tgatcgcggc cggcgcatgg ttcgcgttgg gcacgggatt
     3421 ccgctcggga catcggagcg cgattctttc ggcaagcatg ccgaaagacg aattcgagca
     3481 acggatacaa gcctatcttc tggatcatcc cgaggtgatc gtcgaatccc tcaatcggat
     3541 ggaggaacgc caacgcgcgt ctgcagaaac ggaagttcaa gcgatcattc agagtcgcgc
     3601 cgacgaattg ttccgcgacg tcgacggccc gatcgccggt aatcccaacg gcaacgccac
     3661 gctcgtcgaa ttcttcgatt acaactgccc ctattgccgg cagatggtgc cggtgatgac
     3721 gcaggcggag agcgcggacc ctcagctacg catcgtctac aaagagttcc cgatcctcgg
     3781 acccaattcc aagcttgcgg ccaaggctgc gcttgcggcc cataagcagg gcaaatacct
     3841 cgcgtttcac aaagcccttt ttcaagttcg cggaaccgtt gatccgagca aggtcgcgga
     3901 agtcgccgcc gcggtcggcc tcgacatgga tcgactgaaa gccgacgtgg aggatcccgc
     3961 gatcgcggcg ctgatcgaga aaaatctagc gctcgcgcag gcgctgcaca tcgacgggac
     4021 cccgggattc gtggccggcc gccacgtcct tcgcggtgcg gtcgacctga aagcgcttca
     4081 ggggttcctc caggaagcga gaagcaaccc gtgatggacc aagtatggcc ggcaggctca
     4141 gcaacgctta gggggtctaa taccagcctg cggagacgtg actatcggcg ccatcgtctg
     4201 cgccgagcgc ggcgccaata atcgcgaccg gcttttagag ttttagagtt ttcccgacgt
     4261 atagacagct gcttctacgc cacccatctg aaattgctgg aggctatgga cagcattatc
     4321 gaaagattcg gtcatgcaac aatggtaagg ccgttaggcg aaggtgtgca gaccatcggc
     4381 gattatgatt ttcactgacg acggcggcgc tcggtgga
//
LOCUS       DATLJT010001335         1684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4244618,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001335 DATLJT010000000
VERSION     DATLJT010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4244618"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VM910_30650"
     CDS             complement(<1..972)
                     /locus_tag="VM910_30650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVI66903.1"
                     /translation="MIWPALWSVAGLLALAPAAIPLAMYKRGSAVVYGASLILTSTLC
                     MVAAHNLLNLSHLAWSVTLPVGLPWLGAHFRLDALASFFLIVVNLGGAAASLFGLGYG
                     RHEQSPGRVLPFFPAYLAGMNVVVLADDAFSFLVAWEFMSLTSWALVVSHHREAENVR
                     AGYVYLLMASFGTLALLLAFGVLVGGSGQYDFAAIRGAHPTANVASAVLLLALIGAGS
                     KAGLMPLHAWLPLAHPAAPSHVSGLMSGVMTKVAVYGFVRIVFDLLGPPEWWWSMVVL
                     GVGGPSATLGVLYALMQRDMKRALAYSTIENIGIVFVGLGLALAFKAE"
     gene            complement(1013..1279)
                     /locus_tag="VM910_30655"
     CDS             complement(1013..1279)
                     /locus_tag="VM910_30655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI66904.1"
                     /translation="MITATQLRAARALLGIDQRELAQRCGLSLPTIQRMEASEGVIRG
                     TVDSLVKLVDALAGAGIELIGEGATSSGGGRGVRLKAPNRKRAT"
BASE COUNT          359 a          540 c          513 g          272 t
ORIGIN      
        1 ttccgccttg aaggcgagcg cgaggccgag cccgacgaag acgatgccaa tgttctcgat
       61 cgtggaatag gccagcgcgc gcttcatgtc gcgttgcatc agcgcataga gcactcccag
      121 cgtcgccgaa ggaccgccga cgccgagcac gaccatgctc caccaccatt ccggcggccc
      181 gagcaggtcg aacacgatgc gcacaaagcc gtaaaccgcg accttggtca tcacaccgct
      241 catcaggccg gagacgtggc ttggcgcggc cgggtgcgcg agtggcagcc aggcatgcag
      301 cggcataagg ccggccttgg agccggcgcc gatcagcgcc aacagcagca cggcgctcgc
      361 gacatttgcc gtgggatggg caccgcggat cgcggcgaaa tcatactggc cgctgccgcc
      421 aaccagcacg ccgaaggcga gcaacagtgc cagcgtgcca aagctcgcca tcaagagata
      481 gacatagccg gcgcgaacat tctccgcctc gcggtgatgc gagacgacga gcgcccagga
      541 ggtcagcgac atgaactccc aggcgacgag aaaggagaat gcgtcgtccg ccagcaccac
      601 gacgttcatt ccggcgagat aggcaggaaa aaacggcagc acgcggccgg gcgactgctc
      661 atggcggcca tagccgagcc caaacaggct tgccgcggcg ccaccgagat tgacgacaat
      721 caggaagaaa gatgccagcg catcgagccg aaaatgcgca ccgagccagg gcaagccgac
      781 cggcagggtc acgctccagg cgaggtgcga caggttgagc agattgtgtg ccgccaccat
      841 gcacagcgtg gaggtgagga tgaggctcgc accatagacg accgccgaac cgcgcttgta
      901 catcgcaagt gggatcgcgg ccggcgccag cgccaagagt cccgccaccg accacagcgc
      961 aggccagatc atggtcgccc ccctgtctcg tttgcaggtt gcgcgctcat ggtcatgtcg
     1021 ctcgtttgcg gttcggcgcc ttgagccgaa cgccgcggcc gccaccgctc gaggtcgcgc
     1081 cttcgccgat caattcgatg cccgcgccag ccagcgcgtc gaccagtttc acgagtgagt
     1141 cgacagtgcc gcggatcacg ccttcggagg cttccatgcg ctggatggtc ggcagcgata
     1201 gcccgcagcg ctgcgcgagt tcgcgctgat cgatcccaag gagtgcacgg gcggcgcgca
     1261 gctgcgtggc agtgatcatg ggattggctg atttataaac tgtgagagaa tgcaacaata
     1321 tcatatctat gccgatgtac aagacatcag atacgatgct tttaacatca atcggcgccc
     1381 gtaccacggc aggagcacac ggccctaaca gcgtctgcgc gggccatccg cttcggccgc
     1441 aatgcccgct atggtcagga ttacattctc cggcttgatc gcctcgtcga tggtgtcacc
     1501 agaggtactt cgccgcgagc gcgactacga cgccggccgc gacaaccaat atgaccattt
     1561 gcacccaaag attattggcc acgccaccta gcttcctgtt ttcctgcata gccatggtgt
     1621 ttctccccta gagattttcc tgatgttcgc aatcgccgca atttcgtaat gaacagcgat
     1681 tgtg
//
LOCUS       DATLJT010001336         3915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_108256,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001336 DATLJT010000000
VERSION     DATLJT010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108256"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VM910_30660"
     CDS             complement(<1..1672)
                     /locus_tag="VM910_30660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66905.1"
                     /translation="MIIAEKPLEGAMSLVAPDGSIAAKSAERHGGAPYFWFAEVAEPA
                     AGTWHANLALDHAPPDCSTITRAIPVSTRKTDPVSIPSGAFWQVRNSWNHATETLFSA
                     WIAKLFDAPPDQDLNWKAWNEVLRDRSRNFLFNYLGRGEDNVQSGLRPDCADFGYFLR
                     AYFAYKMGLPFGYSNCTRGSGGRAPKCYQWFDMEHPEFTRPPPPPEQEIASATLATAT
                     PPSQPPTIMNLFSRSQSAEPPVLAPMPMAPPKPRRPANFSEYLRDVGDVVHSGTVRVA
                     ASDENTDFYTVALTQEALRPGTVYGDPYGHVMMLVHRVPEVDGKPGVFLAVDAEPDGS
                     ITRKRFWRGNFLFVHDPALGSPGFKHFRPVVRDKNGSLRRLTNAEITGNPQYGDFSLE
                     QSQISTEEFYDRMDDVMSPEPLDPVKAMTDAIASLNEQVKVRVTSIENGRKYQEKQPG
                     EIGMPNGPSIFQTSGAWEDYSTPARDFRLLIAIDVVRGYPDRVARRAAHYAMPNGKSM
                     EDLKRDLQAVLASELASRKITYTRSDGSPWTLSLKEVIDRSADFEMAYN"
     gene            1671..1946
                     /locus_tag="VM910_30665"
     CDS             1671..1946
                     /locus_tag="VM910_30665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66906.1"
                     /translation="MTRSGIPFQGAKGEGKTWISAASPHGPGAASAGEPTVAKMNAAV
                     AAAANRLAKSILTTFLKEPGTAPPPLPTDRTGPDRRIPESNRPKQQI"
     gene            complement(2022..3620)
                     /locus_tag="VM910_30670"
     CDS             complement(2022..3620)
                     /locus_tag="VM910_30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66907.1"
                     /translation="MVHALNWKRSLIASSMLALAAISAPQALAAGKTITAVMHSDLRI
                     IDPGFTTAYITRDHGYMVYDTLLATDSNFKVQPQMADWKVSDDKLTYTFTLRDGLKWH
                     DGTPVTAEDCVASLKRWGRNDNMGQKLMDFTASIEAADAKTIVLKLKEPYGLVLESIA
                     KPSSYVPFMMPKRLAETPPGQQIPEQIGSGPFKFVKAEFQPGVKAVYEKNTDYIPRKE
                     PASWTSGGKVVKVDRVEWITMADAQTAVNALQSGDIDFMENPSFDILPVLEANPDLKV
                     QTLNKFGFQTLGRMNFLLPPFDNVKVRRAALLAMNQKDVLDALVGNSKYYQVCGAFFV
                     CGTPLETEVGADALVKGGNIEAAKKALAESGYDGTPIVIMAPGDVVTLTAQPVVAAQL
                     LRKAGFKVDLQATDWQTVVSRRASQKPVSEGGWNMFFTNWVAPDVMNPVVNFSIGGRG
                     KNGGWFGWSEDPTIEKLKDQFARSSSLEEQKKIAADIQKEAYEQVIYIPLGQFLIPSA
                     WRKSLSGVLDGPATPIFWNIDKSE"
     gene            complement(3730..>3915)
                     /locus_tag="VM910_30675"
     CDS             complement(3730..>3915)
                     /locus_tag="VM910_30675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66908.1"
                     /translation="PASMASESKGRLAGFMMSRFQASGRDEGRFTRMSAMNLESARLH
                     ATCLIRIGQIAIDAAGA"
BASE COUNT          712 a         1226 c         1211 g          766 t
ORIGIN      
        1 ggttgtaggc catctcgaag tccgcgctcc ggtcgatgac ctccttgagg gacagcgtcc
       61 acggcgagcc atcgctgcgc gtgtaggtta tttttcgcga ggcgagctcg gatgcaagca
      121 ccgcctgcaa gtcgcgtttt aagtcctcca tgctcttgcc gttgggcatc gcgtagtgcg
      181 cggcgcgcct tgcgacgcga tcgggatatc cgcgcacgac gtcgatcgca atcaacagac
      241 ggaaatcgcg cgccggagtc gagtaatcct cccaggctcc cgatgtctgg aagatcgagg
      301 ggccattggg catcccgatc tcgcccggct gcttttcctg atattttcgc ccgttttcga
      361 tcgaggtgac gcgcaccttc acctgctcgt tcaggctggc gatggcgtcg gtcatcgcct
      421 tgaccggatc gagcggctcg ggcgacatca cgtcatccat gcgatcgtaa aactcctcgg
      481 tactgatctg cgactgctcg agcgaaaaat cgccgtactg cggattgccg gtgatttcgg
      541 cgttggtcag ccgccgcaag gaaccgttct tgtcgcgcac cacgggccga aagtgcttga
      601 agccggggct gccgagcgca ggatcgtgca cgaacaggaa attgccgcgc cagaaacgct
      661 ttcgcgtgat cgacccatcg ggctcggcat cgacggcgag aaagacgccc ggtttgccgt
      721 ccacctccgg cacgcgatgc acgagcatca tcacgtggcc gtaggggtcg ccgtacaccg
      781 tgcccgggcg gagcgcttct tgcgtcagcg cgactgtgta gaaatcggta ttttcgtcgc
      841 tcgcggcgac gcgcacggtg ccgctatgga cgacgtcgcc gacgtcccgc aaatattcgc
      901 tgaaattcgc cggccgcctt ggcttcggcg gggccatcgg catcggggcc agcactggcg
      961 gctcggccga ctgcgatcgg gaaaacaggt tcatgatcgt cggcggttgc gagggcggcg
     1021 tggcggtggc aagcgtcgcc gatgcgatct cctgttcggg gggcggcggc ggacgcgtga
     1081 actccgggtg ctccatgtcg aaccactggt agcacttggg tgccctgccg ccggagccac
     1141 gtgtgcaatt cgaatagccg aaaggcagcc ccatcttgta ggcaaaatat gcgcgcagga
     1201 agtagccgaa atcggcgcag tcgggacgca gacccgactg cacattgtcc tcgccgcgac
     1261 ctaggtagtt gaacaggaaa ttgcgcgacc ggtcgcgcag gacctcattc caggctttcc
     1321 aattcagatc ctgatcgggc ggcgcgtcga acagcttggc gatccacgcc gagaacaagg
     1381 tctcggtcgc atggttccag ctattgcgca cctgccagaa ggccccggac ggaatggaca
     1441 cgggatccgt cttgcgcgtg ctcaccggga tcgcgcgtgt aatcgtactg caatccggcg
     1501 gcgcgtggtc gagcgctagg ttggcgtgcc atgtccccgc ggccggctcg gcaacctctg
     1561 cgaaccagaa atacggcgcg cctccgtgcc gctcggccga tttggccgcg atgcttccgt
     1621 cgggcgcgac cagcgacatc gcgccctcga gcggcttttc ggcgatgatc atgacgcgga
     1681 gcggcatccc cttccagggc gcgaagggcg agggcaagac ctggatttcg gccgcatccc
     1741 cgcacggccc gggtgccgct tcggccggcg agcccactgt tgcaaaaatg aacgcagctg
     1801 ttgctgcagc agcaaaccgg ctggcgaaaa gcattttgac aacctttctg aaggaaccgg
     1861 gaaccgcccc tccgcccctg cccacggacc ggaccggacc ggaccgcagg atacccgagt
     1921 caaacaggcc caagcagcaa atttgaggca ccatgcaccg aaaggcgcca tgccggggtt
     1981 ccagcccgga gggcaggttc cagcagcctg ggccgcctgc cctactccga tttgtcgatg
     2041 ttccagaaaa tcggcgtcgc cgggccgtcg agcacgccgg aaagcgattt ccgccaggca
     2101 cttgggatga gaaattgccc gagcggtatg tagataacct gctcgtaggc ttccttctgg
     2161 atgtcggccg cgatcttctt ctgctcctcg agggaggagg atctcgcaaa ctgatccttc
     2221 agtttctcga tcgtcgggtc ctccgaccag ccgaaccagc cgccgttctt gcctcttccg
     2281 ccgatcgaga agttgacgac ggggttcatc acgtcggggg cgacccagtt cgtgaagaac
     2341 atgttccatc cgccctcgct cactggcttc tgactggcgc ggcggctcac cacggtctgc
     2401 cagtcggtcg cctgcaggtc gaccttgaaa ccggccttcc tcaggagttg cgcggccacg
     2461 acaggctggg ccgtcaacgt gacgacgtcg ccaggcgcca tgatcacgat cggcgtgccg
     2521 tcatagccgg actcagcgag cgccttcttc gccgcctcga tattgccgcc cttgaccagc
     2581 gcatctgcgc cgacctcggt ctcgagcggc gtgccgcaca caaagaacgc gccgcagacc
     2641 tggtaatatt tggaattgcc gaccagcgcg tcgagcacgt ccttctggtt catcgccagc
     2701 agcgccgcgc ggcgaacctt gacgttatcg aagggcggca acaggaaatt catacgcccg
     2761 agcgtctgga agccgaactt gttcaaggtc tgcaccttga gatccggatt ggcctccagc
     2821 accggcagta tgtcgaacga cgggttctcc atgaaatcga tgtcgccgga ttgcagcgcg
     2881 ttaacggcgg tctgcgcgtc cgccatggtg atccattcga cgcgatcgac cttcacaacc
     2941 ttgccgccgg aggtccagct tgccggctcc ttgcgcggaa tgtagtcggt gttcttctcg
     3001 tagaccgcct tcacgcccgg ttggaactcg gccttgacga acttgaacgg gcccgagccg
     3061 atctgctccg ggatctgctg gccgggcggc gtttcggcga gccgcttcgg catcatgaac
     3121 ggcacatagg acgacggctt tgcaatcgat tccagcacga gcccataggg ctccttcagt
     3181 ttcagcacga tggtcttggc gtcggcggcc tcgatcgagg cggtgaagtc catcagcttc
     3241 tggcccatat tgtcgttacg gccccagcgc ttcagggagg cgacgcagtc ttccgccgtc
     3301 accggcgtgc cgtcatgcca tttcaatccg tcgcgcagcg tgaaggtgta ggtcaacttg
     3361 tcgtcggaga ccttccagtc cgccatctgc ggctggacct tgaaattgga atcggtcgca
     3421 agcagcgtgt cgtagaccat gtagccatgg tcgcgcgtga tataggccgt ggtgaagccg
     3481 ggatcgatga tgcgcaggtc agagtgcatc acggcggtga tggtcttgcc ggcggcaagc
     3541 gcttgcggcg cgctgattgc ggcgagcgcg agcatcgacg aggcgatgag tgaacgtttc
     3601 cagttcaagg cgtggaccat cgttgtctcc tgacgaaaaa ccggtcaaag caccggtcat
     3661 tgcttatgca tatcgaccgt tgtttgggct cactaacacg ctgtaaatcc tgtcattttg
     3721 agacttgcat caagcgcctg cggcgtcaat cgcgatttgg ccgatgcgaa tgaggcaagt
     3781 tgcgtgcaat cttgcgcttt ccaaattcat cgcagacatt ctcgtaaagc gcccctcatc
     3841 ccgcccggaa gcttgaaagc gagacatcat gaacccggcc aaccttccct tcgattccga
     3901 ggccatgctg gcagg
//
LOCUS       DATLJT010001337         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4244756,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001337 DATLJT010000000
VERSION     DATLJT010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4244756"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VM910_30680"
     CDS             <1..424
                     /locus_tag="VM910_30680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVI66909.1"
                     /translation="RPRSYAQLAYHIFNIADAFLEHEVQDLPLKEGAYGRVPPPEMNT
                     KAKILAYGQDVLGRFRAWWDGPGQTTDFSRKAHVYYGNVTLLEYLERTTWHSGQHVRQ
                     LVMVLEMQGIEPDRPPTKETFAGLPMPDKVWDDERVIA"
     gene            complement(491..1447)
                     /locus_tag="VM910_30685"
     CDS             complement(491..1447)
                     /locus_tag="VM910_30685"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66910.1"
                     /translation="MAKLRIGLLALILAISTSAAAQEWPNRPIRFIVPFPAGGSTDVG
                     ARLVGDYLSRSLRQQIVVENKSGANGNIGMESAAKSAADGYTVLVTTDALSSNPHAYK
                     MNIDPLKELAPVVQLSRQPIVLAAHPSLGVASLAELIALAREQPGLRYATGSGLGAQH
                     MVVQWFASIAGIKLEHVPYRGGTPALNDLIAGHVKLGSLGSTPLVPHYKAGTLRLLAQ
                     TTEKRSPSLPDVPTYQEAGIKGLVLDQWVGVFVPAGTTPAIGMRLNTEINKALAEAAI
                     RDGFLQSGQEPVGGSADAFSQLVHGDFETYARLVKELNIKAD"
     gene            1754..>2240
                     /locus_tag="VM910_30690"
     CDS             1754..>2240
                     /locus_tag="VM910_30690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HVI66911.1"
                     /translation="MPFSTYLPEHMGSIRLTPSGPFVAGAYAELTLTYTAGTFGIDDT
                     GMVKISWRTTSDMSKPQFDKPAAPNFTTVEASNGAKLDVWFDRLNIRPYANTLLIRVG
                     RGYLRAGDTLTVRLGDRRQGSPGYRLQTNCEARVELRTSIDAFATYEFTELPEQPAFA
                     LV"
BASE COUNT          390 a          724 c          710 g          416 t
ORIGIN      
        1 tcggccgcgc agctacgccc agctcgccta tcacatcttc aacatcgccg atgccttcct
       61 cgagcacgag gtgcaggact tgccactcaa ggaaggcgcg tacggccgtg tgccgccgcc
      121 ggagatgaac accaaggcca agattctagc ctacggacaa gacgtgttgg ggcgattccg
      181 agcctggtgg gatggcccag gccagacgac cgatttttcg cgcaaggccc acgtctacta
      241 cggcaacgtc acgctgctcg agtatttgga gcggaccacc tggcattcgg gtcagcacgt
      301 gcgccagctc gtgatggtgc tcgagatgca gggcattgag ccggataggc cgccgaccaa
      361 ggaaaccttt gccggcctgc ctatgcccga caaggtctgg gatgacgagc gggtcatagc
      421 ctgatgtggg cacaacaacg acgctggccg cgcttgcggc ccgatgtctc gcctggacat
      481 ttttgtaggc ctaatcggct ttgatgttga gctccttcac caggcgtgcg tacgtctcga
      541 aatcgccgtg aacgagttgc gagaatgcgt cggcgctgcc gccgaccggt tcctggccgg
      601 attgcaggaa gccgtcgcgg atcgccgcct cggcgagcgc cttgttgatc tcggtgttga
      661 gacgcatgcc gatcgccggc gtcgttcccg ccggcacgaa caccccgacc cattgatcga
      721 gcaccagccc cttgatccct gcttcttgat aggtcggcac gtcgggcagg ctcggcgaac
      781 gcttctctgt tgtttgtgcg aggagccgca gggtgccggc cttgtagtgc gggacgagcg
      841 gcgtcgaccc gagcgatccg agcttcacgt gcccggcgat gagatcgttg agcgccggcg
      901 tgccgccgcg atagggcacg tgctcgagct tgatgccagc gatcgaggcg aaccattgca
      961 ccaccatatg ctgggcgccc aatccgcttc cggtcgcgta gcgcagccct ggctgctctc
     1021 tggcaagtgc gatcaactcg gcaagcgagg caacgcccag agaggggtgg gccgcgagca
     1081 cgatgggttg gcgcgagagc tggacgaccg gtgcgagttc cttcagtgga tcgatgttca
     1141 tcttgtaggc gtgcggattg ctgctcaagg cgtcggtcgt aaccaggacc gtgtagccat
     1201 ccgccgcgct cttggccgcg ctctccatgc cgatattgcc gttggcaccg gacttgttct
     1261 cgacgacgat ttgctgacgc aggctgcgcg acagatagtc gccgacgagg cgcgcgccca
     1321 cgtcggtcga gcccccggcc gggaacggca cgatgaagcg aatcggccgg ttcggccatt
     1381 cctgcgcggc tgcgctcgtc gaaatcgcga ggatgagagc cagcaggccg atgcgcaatt
     1441 tcgccattgg aaaccgatcc cgtgcttctc ggtaccactg cgctgaaaag tgacgtcgca
     1501 ccttttatta gagccgatgc tctgccgaca agctcggtcg gctccctctc ccatagggag
     1561 aggggtgggg tgaggggtta aggtctctcg gtgggcttcg aaccccctca ccccaaccct
     1621 ctccccaacg gggagaggga gcgcaccgcg tccgcggtgc gagtgcgggc ttgtactgta
     1681 tcagcgaatt gcaaatatcc tatcgatcct tcttccgtcc gactagcagc gatgccgcga
     1741 tcgaggtccg cgcatgccgt tctccaccta tctccccgag cacatgggct cgatccggct
     1801 cacgccatct gggccctttg tcgccggcgc gtacgccgag ctgaccctga cctacaccgc
     1861 gggcacgttc ggcatcgacg ataccggcat ggtaaaaatc tcgtggcgga cgacctccga
     1921 catgagcaag ccgcaattcg acaagcctgc ggcgccgaac ttcaccacgg tcgaggccag
     1981 caacggtgcc aagctcgacg tctggttcga ccgtctcaac atccgcccct acgcgaacac
     2041 gctgctgatc cgcgtcggcc gcggctatct gcgcgccggc gatacgctca ccgtgcgcct
     2101 gggcgaccgc cggcagggct cgcccggata ccggctgcag accaattgcg aagcgcgcgt
     2161 cgagctcagg acgtcgatcg atgcgtttgc gacctatgaa ttcaccgagc tgccggagca
     2221 gccggcgttt gcgctggtgc
//
LOCUS       DATLJT010001338         6563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2177460,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001338 DATLJT010000000
VERSION     DATLJT010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2177460"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VM910_30695"
     CDS             complement(<1..320)
                     /locus_tag="VM910_30695"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66912.1"
                     /translation="MLVPSPKWLDSGNNAWQLAAATFVGLQSIPGLAVLYAGYVKQKW
                     AINSAFMCFYAFASVLVVWVLFDYNMAFGEQWFPFLGKPALATSATFITGQAVIPAAA
                     SGMP"
     gene            561..965
                     /locus_tag="VM910_30700"
     CDS             561..965
                     /locus_tag="VM910_30700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66913.1"
                     /translation="MRAWLFNKPGFGYGSAHSDVDATMFQNPVLAARIAGLVAGLAIP
                     TVNADAQESKTAITCINPMSGTSWQIAIDFDRATVNSNRAEITAAKIAWFDPTDGSNY
                     TLDRKSGALTAIVASSTGGYFRHARCSLEKSR"
     gene            1052..2758
                     /locus_tag="VM910_30705"
     CDS             1052..2758
                     /locus_tag="VM910_30705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVI66914.1"
                     /translation="MATVETDVRASVGFASKTGPRKANEDFAGAVSGAELPEPRQEVI
                     AAIADGIGGAKGGRVAAETAVRGFLDGFCDLPETMEVQRAGARIVSALNGWINTMGRQ
                     DPKLAGMGCTLTALVLRGRLAHLLHVGDTRAYRLSGNRLTCLTTDHVRQDGRGRSHTL
                     NRALGVEAEVRLDYATQPVTLHDRFLLCSDGVHGYLTEERIADILRERSAPEDTAGAL
                     VTAALQYGSTDNCTALVLDVVALPTAGSADVSAAILHLPVIPVPIVGETIDGFVLSAL
                     ISDGRYTRLFGAQDQIEGGTVALKFPKPQIASVEAYHAAFVREAWVGARVHNPWVGRV
                     IELPPGRQTCLYTVMPLYQGELLETRLARSPALGLEEGRNITVKLARAVAALHRVGII
                     HRDIKPDNVILESGGSLKLIDLGVVRVPGLEEFPPENIPGTAAYMAPEMFTGEPGNEA
                     TDIYALGVTMFRAFTGEFPYGNLDATSPPRRNRPRDFSALRPDLPAWLQTALARASAV
                     DPASRFHDMAEFAFEMEAGPARAPIENRRPRTLYERAPLQFWQGVAALLALALLVSLL
                     VR"
     gene            complement(2902..4173)
                     /locus_tag="VM910_30710"
     CDS             complement(2902..4173)
                     /locus_tag="VM910_30710"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI66915.1"
                     /translation="MNKLKKGIINGAAAAWVVLGAGATVALADDQPAPSGEQPPPPAE
                     QPAAPTAMTTPALTGPLVANPNPISFDAGFLGPVYYNGFVSGLGMWQNNVFPGDQHVL
                     ASLSNGQFSFQKTDGLFQYYVQVGAYTIPDLGVPYFNVLTTTGDFFGPIPIAWAKLAP
                     TDNFNIQGGQIPTLIGLENTFSFQNMNIERGLLWNQEPAISRGGQLNYTQGPLALALS
                     WNDGFYSNNFTWLTGSATYTLDPANSFVLAAGGNYSHTTKVTTTTPFFQTTATPLLQN
                     NETLFTLGYTYNSAPWTVTPYFQFTHVPASSSPPISTFSSGSTIGGAILANYSFGDDS
                     PLPGFSLPVRFEYIGSTGSLANGAPNLLYGPGSNAWSITFTPTYQYKIFFARAEISHV
                     GTGSTTPGLIFGPNGTNTTQTRGVLEVGILF"
     gene            4727..5962
                     /locus_tag="VM910_30715"
     CDS             4727..5962
                     /locus_tag="VM910_30715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVI66916.1"
                     /translation="MPAPGGVNTGDTAWMLTSTALVLMMTIPGLALFYGGMVRKMNVL
                     ATVMQSFAVTCLVTVLWMVVSYSLAFTEGSSFVGGLSRFLLLGMTLDSVNDLARTIPE
                     SVYMTFQMAFAIITPALICGSFADRMKFSALLWFIGLWAVLVYAPIAHWVWGPDGFLN
                     DAGVLDFAGGTVVHVNSGVAGLMAALVLGKRRGFGTEPMAPHNLVLSVIGASLLWVGW
                     FGFNAGSATAANGNAGMAMAVTQIATATAALAWMFAEWIVKGKPSVLGIISGAVAGLV
                     AITPASGFVDPRGALIIGIAAGVVCYWGTTGLKHALGYDDSLDVFGVHGVGGAVGAFL
                     TGMLAVAWVGGEGKSGLIDGNPHQVITQLYGITVVAVYDAIASLILLKLVDVTIGLRV
                     DTEIEREGLDLAIHGEAVQ"
     gene            6080..6418
                     /locus_tag="VM910_30720"
     CDS             6080..6418
                     /locus_tag="VM910_30720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVI66917.1"
                     /translation="MKKIEAIIKPFKLDEVKNALHEVGVTGMTVTEVKGFGRQKGHTE
                     VYRGAEYVIDFLPKLKVEVVLDDSLVERAVQAVQDAARTDKIGDGKIFVVPVEQAVRI
                     RTGEQGPDAV"
BASE COUNT         1301 a         1893 c         2073 g         1296 t
ORIGIN      
        1 tccggcatgc ccgaggccgc tgccgggatc accgcttggc cggtgatgaa cgtcgcagag
       61 gtcgccaacg ccggtttgcc gaggaacgga aaccactgct ctccaaacgc catgttgtag
      121 tcgaatagaa cccacacaac caaaaccgat gcgaaggcgt aaaagcacat gaaggcagaa
      181 ttgatcgccc atttctgctt cacatacccg gcgtagagaa ccgccagacc gggaatgctt
      241 tgcaggccga cgaaagttgc ggccgccaac tgccatgcat tgttgccgga atcgagccat
      301 ttgggtgatg gtacgagcat aagcttatcc cccgttattc tgttacgacg aacctcacaa
      361 ccaccggtcc ccaaccgtgg ggcttgttaa gcgcaccaag tgaggttgca gaggcaaaag
      421 ctatgccaga cgtaggaccc tgatcgcaag ccctggcgag acctccctat ttaaggaagg
      481 cgatgtcata tcgttgattg gcgctagcac caagtgcaca aaatctaggc agagctgtcg
      541 ccggctctta gatgagtgga atgcgcgcct ggcttttcaa caagccgggg tttgggtatg
      601 gatcggcgca tagtgatgtg gacgcaacca tgttccaaaa cccggttcta gcggcgagaa
      661 tagccggctt ggtagcgggt ttggccattc cgacggtcaa cgctgacgcg caggagagta
      721 aaaccgcgat cacttgcatc aatccgatga gcgggacgtc ctggcagata gcgatcgact
      781 tcgatagagc cactgtcaat tccaatcggg ctgagattac cgctgcgaag attgcctggt
      841 tcgatcccac ggacggcagc aactacacgc tcgaccgtaa atccggcgct ctcacggcga
      901 ttgtcgcgtc gagcaccggg ggctattttc gccatgctcg ttgcagtctg gagaaatcgc
      961 ggtgacggcg tgaatgtgcc actgcgccgc cgccccggcg ccactatttc gctacgaccc
     1021 agccaaccgc cgcccattcg gaattggtgc gatggcgacg gttgagaccg acgtacgggc
     1081 aagcgttgga ttcgcgtcga agaccgggcc gcgcaaggcc aacgaagatt tcgccggcgc
     1141 tgtgtcaggc gcggagttgc ccgagcctcg ccaggaggta attgccgcga tcgccgatgg
     1201 catcggcgga gcgaagggcg gtcgcgttgc ggccgagacc gcggtgcgcg gcttcctgga
     1261 cggattttgc gatctgcccg agaccatgga ggtgcagcgg gcaggtgcca gaatcgtcag
     1321 cgcgctcaat ggctggatca ataccatggg ccggcaagac cccaagctcg ccggaatggg
     1381 gtgcacgtta acggcgctgg tgctgcgcgg ccgcctcgcc cacctgctgc acgttgggga
     1441 cacgcgcgcc tatcggttga gcggcaaccg cctgacttgc ctcaccaccg accatgtgcg
     1501 ccaggatggc agggggcgct cgcatacgct caaccgcgca ctgggagtgg aggcggaagt
     1561 gcgccttgat tacgccacgc agccggtgac actgcacgat cgtttcctgc tgtgcagcga
     1621 cggcgtgcat gggtatctga cggaagagag aatcgccgac atcttgcgcg aacgctccgc
     1681 tcccgaggat accgccggtg cgttggttac agccgcgctc caatacggta gcaccgacaa
     1741 ctgcaccgcc ttggttctgg acgtggttgc gttgccgaca gcgggatcgg ccgacgttag
     1801 cgccgccatc cttcacctgc cggtgatccc ggtgccaatc gtcggcgaga cgatcgacgg
     1861 atttgtccta agtgcgctga tctccgacgg ccgatacact cggctattcg gcgcgcagga
     1921 ccagatcgag gggggtaccg tagccttgaa gttccccaag ccccagatcg ccagcgtcga
     1981 ggcgtatcac gcggcgttcg tccgcgaggc ttgggtcggc gcgcgagtgc acaatccctg
     2041 ggtaggacgc gtcatcgagc tgccgccggg acgccagacc tgcctgtaca cggtcatgcc
     2101 gctctatcaa ggcgaacttc tggaaacgcg gcttgcgcgc agtcctgcgc tgggattgga
     2161 ggagggtcgg aacatcaccg tcaagctggc ccgcgcggtg gcggcgctac atcgcgtcgg
     2221 catcatccat cgcgacatca aacccgacaa cgtcatcctc gaaagcggcg gatcgctgaa
     2281 gctgattgac ctcggggtgg ttcgcgtgcc tgggctggag gaatttccgc cggagaacat
     2341 tcccggcaca gccgcctata tggcgcccga aatgttcact ggcgaacctg gcaatgaggc
     2401 caccgacatt tatgcgctgg gagtcaccat gttccgcgct ttcaccggcg aattcccgta
     2461 cggcaatctc gacgccacca gtccgccgcg ccgcaaccgg ccacgggatt tttctgctct
     2521 gcgaccggat cttcccgcct ggctccaaac cgcgctcgcg cgcgcgagcg cggtcgaccc
     2581 tgcgagccgc tttcacgaca tggccgagtt cgcgttcgag atggaagccg ggccggctcg
     2641 cgccccaatc gaaaatcgcc ggccgcggac gctttatgag cgcgccccgc tccaattctg
     2701 gcaaggagtg gccgctttgt tggcgctggc acttctcgtg tccttgctgg tgcgttaagc
     2761 cgtagggctt tccgcgtcag cgcccggtca tggcgaggaa gttggacatt cgctcgcgca
     2821 gtcggggcga gcggacccga cgggcgccgt gcttcccccg gatgtgcccc gcgttaggcc
     2881 cgcggtggcc cactcgccgg ctcagaacag gatgcccacc tcgagcacgc cgcgtgtctg
     2941 ggtcgtgttg gtgccatttg ggccgaagat gagaccagga gtcgtgctgc cggttccgac
     3001 atgggaaatc tctgcacgag caaagaaaat cttgtattga taagttggcg tgaaggttat
     3061 cgaccacgcg ttgctgcccg gaccgtacaa aagattgggc gcgccattag ccaggctgcc
     3121 ggtcgagccg atatactcga agcgaaccgg caggctgaaa cccggcaaag gcgaatcgtc
     3181 gccaaagctg taatttgcca agatcgcccc gccaatcgtc gatcctgagg agaatgtgct
     3241 aatcggaggc gacgacgagg ccgggacatg ggtgaattgg aaataaggcg taaccgtcca
     3301 gggcgctgag ttatacgtat agcccaacgt aaagagtgtc tcattgttct ggagcaaagg
     3361 agtcgcagtt gtttgaaaga aaggagtcgt cgtcgttact ttagtagtat gagagtaatt
     3421 gccgcccgca gcgagaacga agctgttggc aggatcgagc gtataggtgg ctgagcccgt
     3481 tagccatgta aagttatttg agtaaaaacc gtcattccat gacagcgcca aggcgagagg
     3541 gccctgagta tagttcaatt gaccgccacg actgatcgcc ggctcttggt tccacaagag
     3601 accgcgttcg atattcatgt tctggaagga aaaggtattc tcaaggccga tcaaggtcgg
     3661 gatctggcct ccttgaatat tgaaattatc agtgggcgcc agctttgccc acgctattgg
     3721 gatcggtccg aagaagtcac cggtcgtcgt cagcacgttg aaataaggca cgccgagatc
     3781 gggtatcgta tacgcgccga cctgaacgta atattgaaag agaccgtcgg ttttctgaaa
     3841 agaaaattga ccgttgctca gactcgccag cacatgctga tcaccgggaa agacgttatt
     3901 ctgccacatt ccaagaccgc tgacgaagcc gttataatac accgggccga gaaacccggc
     3961 gtcgaaactg atcggattcg ggttcgccac aagcgggccg gtcagggcag gtgtcgtcat
     4021 agccgtcggg gctgccggct gttcggctgg aggcggaggt tgctcaccag agggtgccgg
     4081 ctgatcgtcg gccagcgcga cagtggcgcc agcgccgagc acaacccaag cggcggcagc
     4141 gccattgatg atgcccttct tcagcttgtt cattttgagg acctttcgaa atcaccgttc
     4201 ctgtggagca cacgatcctg accatggcgg tccgccaact tgaagcaagc ggcgtaccac
     4261 ccgcctcgcg gagacgacac attgcgaccg aaccggggag ttgcgcgccg acttacaagt
     4321 tctcgttctc ctcgcaagtt gggcacgtgt caactgtcgc tttggcgaga tcagaataga
     4381 ggatggctta cgcatgctat tttggattgc tgctcaaaag gactgctggt tgttgcagtg
     4441 tgactaaaga ttaggcaaga ttgagattga ccgaaggaga atcgcgatcg acgcggaatt
     4501 taggcatctt ggcacgattc ttgggttcgg acaggagact tccggacccg taccccctga
     4561 cgcaaaccgc gcctgcgtcg gatggtcggc atacctggtg tttggaggta tgggatcgat
     4621 gggtgaattt ctgcgccgaa cattgtttgc catgtcgatc acgttcctct gggctgtcac
     4681 ggcgctggcg cagctgacgc cggaggctgc aacacctgcg gtcgcgatgc cggcccccgg
     4741 cggggtcaac accggcgaca cggcgtggat gctgacctcg accgcgctcg tactgatgat
     4801 gacgattcct ggtctcgccc tgttctatgg cggcatggtc cgcaagatga acgtactggc
     4861 gacggtgatg cagagctttg ccgtgacctg cctcgtcacg gtcttgtgga tggtggtcag
     4921 ctacagcctc gccttcaccg aaggatcgtc gttcgtcgga gggttgagcc gtttcttgct
     4981 gctcggcatg accctcgact cggtcaacga cctggccagg acgatcccgg aatcggttta
     5041 tatgactttc cagatggcct ttgcgatcat caccccggcg ctgatctgcg gctccttcgc
     5101 agaccggatg aagttctcgg cattgctgtg gttcatcggg ctgtgggcgg ttcttgtcta
     5161 cgcgccaatc gctcactggg tatggggacc cgacgggttc ctcaatgacg ccggtgtgct
     5221 cgatttcgcc ggcggcaccg tggtgcacgt caattcgggg gttgccggac taatggccgc
     5281 actggtgttg ggcaagcggc gcggctttgg gacggagccg atggccccgc acaatctggt
     5341 gctcagcgtg atcggcgcct cgctgctgtg ggtcggctgg ttcggcttca atgcgggttc
     5401 agcgacggcg gccaacggca atgccggcat ggcgatggcg gtgacccaga ttgcgaccgc
     5461 gactgctgct cttgcctgga tgttcgccga gtggatcgtc aaaggcaagc cgagcgtgct
     5521 aggcatcatc tcgggcgcgg tcgccgggct ggtcgcgatc actccggcgt cgggttttgt
     5581 cgatccgcgt ggcgcactga tcatcggcat cgctgccggc gtcgtctgct attggggtac
     5641 caccgggctc aagcacgcgc tcggctatga cgacagtctc gacgtcttcg gcgtgcatgg
     5701 cgtcggcggt gccgtcggcg ccttcctgac cggcatgttg gctgtagcct gggtgggcgg
     5761 cgaaggtaag agcggcctga tcgacggcaa tccgcatcag gtgattacgc agctatatgg
     5821 cattacggtc gtcgcagtct atgacgcgat cgcctcgctg atcctgctca agcttgtaga
     5881 tgtgacgatc gggctccgtg ttgacaccga aatagaacgc gagggcctcg acctcgcgat
     5941 tcacggtgag gcggtacaat gaccggaggt ttagccatgt cgccgcttga gggggccgac
     6001 cgggcccgcc atttcaatgg cattgggcga aagtgcataa cccgaggcgg caccgagctc
     6061 aattcgagat ggtgagacca tgaaaaagat cgaagcaatc ataaagccct tcaagctcga
     6121 tgaggtgaag aacgcgttgc acgaagtcgg cgtcaccggc atgactgtta ccgaggtgaa
     6181 gggcttcggg cggcagaagg gtcacaccga ggtttatcgt ggggcagagt acgtgatcga
     6241 cttcctgccc aagctcaagg tcgaagtcgt gctcgacgat tctctcgtcg aacgcgccgt
     6301 gcaggcggtt caggacgccg cccgcacaga caagatcggc gacggcaaga tatttgtagt
     6361 gccggtcgaa caagctgtcc gcattcgaac cggggagcaa gggcccgatg ccgtttgaca
     6421 tcgggtttgc caaccagcca cgcgtccaac cgagctaagg gagcgctgat gacctaccac
     6481 tgcaagacgc ccgaggatgt actgaaggcg atcaaggacg acaagatcgc gatggtcgat
     6541 ctgcggttca ccgatctgcc ggg
//
LOCUS       DATLJT010001339         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_796616,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001339 DATLJT010000000
VERSION     DATLJT010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796616"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="terL"
                     /locus_tag="VM910_30725"
     CDS             complement(<1..1373)
                     /gene="terL"
                     /locus_tag="VM910_30725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HVI66918.1"
                     /translation="MNSNLTRRLINSPWYAGHWSSRFRLREDQNTKMQFDTDAGGSRL
                     ASSVGGSLLGLGGDVLIVDDPHNTESVESEAERETVLTWFKELSTTRLNDPKQAALVV
                     IMQRLHEADVSGHILSSGDGEFVHLMIPMEYEWHRHCVTSLGWHDPRGVSDDGEPLLE
                     MKADGPVPLDAQAERVLEQERNGALMWPERFGAAEVASMHAGLGPYLASGRLQQSPQP
                     RGGGLFRREWWQLWDPPDGKFPLCDLVIGSVDGAFTEKEENDPSAMTVWALFKHPERG
                     RRRIILIDAWRKFLPMHGNPTPRLDHEVIRPGDSLPVVRAKNARWRQRIGHEWGLVEW
                     VAETCRAWGVEKLLIEGKASGITAAQELQRLYGREPWSVQLINPKSDKIARALAVQPL
                     FANGQIYAPARDWSELVIDEMMMFPRGRYDDLTDTTSMALNYLRSVGEARTDVEVKAD
                     EIGTVMH"
     gene            complement(1544..>2221)
                     /locus_tag="VM910_30730"
     CDS             complement(1544..>2221)
                     /locus_tag="VM910_30730"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66919.1"
                     /translation="AAAWPLAARAQQPMAKLPTIGFLGAATPSAWSQRVAAFLQRMHE
                     LGWTEGRTVVVEYRWAEGRPERFLEIAAEFARLKVDVIIAAGTPAVLAAKQATSVVPI
                     VFVGAGDPVGTGLVASLPRPGSNVTGLSSQAVDTVGKRLELLRELMPGIRRLAIMGNV
                     DTSSVSFVPAVLSFKRLPPSGATTRRRSGWREASSILSRRHGHRSTPPSTSRAGRSMR
                     FVNICKP"
BASE COUNT          413 a          748 c          668 g          392 t
ORIGIN      
        1 ttgtgcatca cggtgccgat ttcgtcggcc ttcacctcga cgtcggtacg cgcctcgccg
       61 acgctgcgaa gatagttcaa ggccatcgaa gttgtgtcgg tcaggtcatc gtagcggccg
      121 cgggggaaca tcatcatctc gtcgatcaca agctcgctcc agtcccgcgc gggcgcataa
      181 atctggccgt tggcgaacag cggttgcact gccagcgcac gggcgatctt gtcggacttc
      241 ggatttatca actgcaccga ccagggctct ctgccgtata gccgttggag ttcctgcgcg
      301 gcggtgatgc cgctcgcctt gccttcgatc agcagtttct ccacgcccca ggcgcgacag
      361 gtctcggcga cccactcgac caagccccat tcgtgaccga tacgctgccg ccagcgcgcg
      421 tttttcgccc gaaccaccgg cagcgaatcg cccggccgga tcacttcgtg gtcgagacga
      481 ggcgtggggt tgccatgcat gggaaggaat ttccgccagg cgtcgatcag aataattcgg
      541 cggcgtccac gctctggatg tttgaaaagc gcccaaacgg tcatggcgct cggatcgttt
      601 tcctccttct cggtgaacgc accgtcgacc gagccaatca ccaagtcgca aagcgggaac
      661 ttgccgtcgg gcgggtccca caattgccac cactcccggc gaaacaggcc gccgccacgc
      721 ggctgcggac tttgctgcaa acgcccggaa gcaagataag gaccgaggcc agcgtgcatc
      781 gaggccacct cggcggcacc gaaccgctcc ggccacatca gcgcgccgtt gcgctcctgc
      841 tctagaaccc gctccgcctg cgcatcgagc ggcaccggcc catccgcctt catctcgagg
      901 aggggctcgc catcgtcgga aaccccgcgg gggtcatgcc agcccagcga cgtcacgcag
      961 tgccgatgcc actcatactc catcggaatc atgagatgca cgaactcgcc gtcgcccgaa
     1021 gacaagatgt ggccggatac gtcagcctca tgcagtcgct gcatgatcac cacgagggca
     1081 gcctgcttgg gatcgttcaa ccgcgtggtg gatagctcct tgaaccaggt gagcacggtc
     1141 tcgcgctcag cctccgattc gaccgattcg gtattgtggg ggtcgtcgac gatgagcacg
     1201 tcgccgccga ggccgagaag tgagccgccg acggagcttg cgagcctcga gccgcccgca
     1261 tcggtgtcga attgcatctt ggtgttctgg tcctcgcgca atctaaaccg cgatgaccag
     1321 tgccccgcgt accacgggct attgatcagc cggcgcgtca ggttgctgtt catcaacgac
     1381 agcgtgtgac tgtaactccc gcacaggaac ctgacctgcg gcccagatcg gtaggattgc
     1441 caacgtcgcg accatgtcca tgccggccag cacaccgagg tgaccagtgt ctttgcgcac
     1501 cgcggcggga agttgaccaa caatttcttg atctgtccgt cggttacggc ttgcaaatgt
     1561 tcacaaagcg catcgatcgc ccagcacgac ttgtactcgg cggcgtcgag cgatggccat
     1621 gccgcctcga caaaatcgat gaggcttccc tccagccgct gcgcctcctc gtcgtcgcgc
     1681 ccgagggcgg caatcgcttg aagctcagca cggcgggcac gaacgagacc gaggaggtgt
     1741 ccacattgcc catgatcgct aaccgacgga tgccaggcat gagctcgcgc aacaattcga
     1801 gccgcttgcc gacggtatcg acagcctggc tcgacagccc ggtgacgttg ctgcccggtc
     1861 gcggaagact agcgacaaga ccggtaccca ccgggtcccc cgcgcccacg aacacaatcg
     1921 gcacgaccga ggttgcctgc tttgcggcaa gaaccgccgg ggttccggct gcgataatga
     1981 catcgacctt gagccgggca aactcggcag cgatctccag gaaacgctcg gggcgtccct
     2041 ctgcccagcg atattccacc acgacggtgc gaccctcggt ccaaccgagc tcatgcattc
     2101 gctgcaggaa ggcggcgact cgctggctcc aggctgaagg cgtggccgcg cccaggaagc
     2161 cgatggtcgg cagctttgcc atcggttgct gcgcgcgcgc cgcgagcggc cacgctgccg
     2221 c
//
LOCUS       DATLJT010001340        10455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_796875,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001340 DATLJT010000000
VERSION     DATLJT010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796875"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..463)
                     /locus_tag="VM910_30735"
     CDS             complement(323..463)
                     /locus_tag="VM910_30735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66920.1"
                     /translation="MLKDFEPVSLLTTNPLLIVTNKAVPAKDLKELILWLKGQCISNA
                     GF"
     gene            complement(549..950)
                     /locus_tag="VM910_30740"
     CDS             complement(549..950)
                     /locus_tag="VM910_30740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66921.1"
                     /translation="MISMTFTGPPFLEKLSFFTIATAKTDTSIQNNSDLSQGLWSAIG
                     GTVDHKDGKHLDASWEPKGSTSMNMSVTEFVVPLAIVGRNRSVWREIIDTVMEGPPRT
                     AADEITEYLARHQYDLSPTLRIELERRHVCL"
     gene            1178..1402
                     /locus_tag="VM910_30745"
     CDS             1178..1402
                     /locus_tag="VM910_30745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66922.1"
                     /translation="MLTSDEVQKRNAFAACAAFRIEGLEASLDRAREIAFQGELDAVG
                     HHIEEAQGYLAQIRLLHQEALDEFCRAQGE"
     gene            1888..4269
                     /locus_tag="VM910_30750"
     CDS             1888..4269
                     /locus_tag="VM910_30750"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI66923.1"
                     /translation="MVSAKKKLALITRELKEARQQQAATTDVLKVISRSTFDLQVVLD
                     TLVESAVHLCDADHAWLFQREGEFFRWVASYGHATDVHERIRDYFRTLPVPVDRGSVT
                     GRTALEAKVVQVPDVLADPEYTWSEAQKLGGYRAALGVPLLRKGNVVGVIFVAKIVPR
                     PFTAKQIELVTTFADQAVIAIENTRLLSELRQRTDDLSESLEQQTAASQVLQVISSST
                     GELEPVFEAILASATRTCDAKFGLLYRIENGAARIISRLGIPPALAEYLKRGPHRPPL
                     NRLDPHTPVGRVVQSRQTVHVADYRTDQAYLNRDPLTVAGVELGGIRTLLVVPMIKND
                     ALIGAIVIFRQEVRPFTDKQIDLVQNFANQAVIAIENARLLNELRESLQQQTTTADVL
                     KVISRSSFDLQAVLDTLTVSAARHCEAQMAAIVRQKDAGSYYYATTYGISADSNNYLK
                     SVAIKVGRGTVVGRALLEGRIVQVPDVLADPEYTWTEAQERTGFRSVLAVPLLREAHP
                     IGALVLARSVTKPFTDKQVEVVTTFADQAVIAIENVRLFDEIQDKSRQLAEASQRKSL
                     FLASMSHELRTPLNAIIGLTEMMIKNAARFGTEKAQEPLQRVNRAGTHLLGLINQVLD
                     LSKIEAGKLELNPQTVQLAPLINEVIGTAGQLAEQNKNRLAVDTQENLGALTVDPMRL
                     RQILLNLLSNACKFTKAGEVKLVARKVSNGSNFVEFVVSDTGIGMMPEQQAKLFEEFS
                     QADAATAQKFGGTGLGLSITRKLARMMGGDVKVTSEAGKGSVFTVRLPAGERT"
     gene            4266..4436
                     /locus_tag="VM910_30755"
     CDS             4266..4436
                     /locus_tag="VM910_30755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66924.1"
                     /translation="MNAMKLPRRQFLHLAAGAAALPAVSPIARAQAYPARPVRAPPRG
                     DFTMAMLDQMNT"
     gene            complement(4717..5271)
                     /locus_tag="VM910_30760"
     CDS             complement(4717..5271)
                     /locus_tag="VM910_30760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66925.1"
                     /translation="MPTGMAPERELLPAQRRAANEVNAWHILMMEIAKPRHVAPRFLR
                     DAEDYVESVRQLAALQGGIVRKWVSPIYQLLCHAIELTLKAYLAASGVPITTLANHIG
                     HDLELAFRCAQKHGFTPSDARFWEVVQSLAPYHLDHSFRYGKPHGYVTHPEPSKAAEV
                     IANTIKGTVEPYVRRQYDDINQGR"
     gene            complement(6289..6951)
                     /locus_tag="VM910_30765"
     CDS             complement(6289..6951)
                     /locus_tag="VM910_30765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66926.1"
                     /translation="MFAVRMSSIASVTLVPAKLIPRNFVEDHRQMPAVTRIVCVVLIA
                     FLSAVTGAAAQTSVKGPVTVFDFTFPEEIAGAQRISFRDYRSIRPGLGYSAGYQQGNL
                     IGTVYIYDDGKQSIPDDPQSPLVKAQLEQSRRDVLGLRQGVRVKVDVKEDFTIEDAHG
                     RTRLICTAFAITGEDGRPRDSFSCVGVAKSKFLKFRISGVSQAEARRFANAWIDLLWP
                     PS"
     gene            complement(6955..7338)
                     /locus_tag="VM910_30770"
     CDS             complement(6955..7338)
                     /locus_tag="VM910_30770"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI66927.1"
                     /translation="MDTRDLHDKGLKLRREMFGRDAVEQRMNAFGAFGEPLQNMINAY
                     AYGDVWSRSALPLKTKSLAAIAMTAAIGRANELRVHVNGALTNGCTAEEIRDILLLVA
                     LYCGIPAAIEGHRIALDVLRERGAA"
     gene            complement(7352..8164)
                     /locus_tag="VM910_30775"
     CDS             complement(7352..8164)
                     /locus_tag="VM910_30775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI66928.1"
                     /translation="MGAAPSDLAVIVTGGARGLGRAMALGLAKDGVRVAVADLPSSQA
                     DMRELAELARAQQVEERIHPIDCDVTRWADCTAAVRNAVEHFGAVHGLVNNAGVGMQH
                     IGNVLVGARKKFYEVDADTWRNAIDVNFNGPFLMAKAIAPTLVAQGWGRIVNIETSSY
                     TMMMEGFSPYGPSKAALEAATVIWAKDLAGTGVSVNALAPGGPANTRMIPATEVADRS
                     TLIQPEVMIAPIVWLMSPRSDGVTGRRIIAKEWDAARLASAPAEKVGTPAGW"
     gene            8323..9393
                     /locus_tag="VM910_30780"
     CDS             8323..9393
                     /locus_tag="VM910_30780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI66929.1"
                     /translation="MKFGIFDYIDARGETLQKTYEDRLALLQAAEAAGFYGYHLTEHH
                     ATPLSMTPSPSVFLAAAARETRRIRLGTLLYLLPLYHPLRLLEELCMLDHLSGGRLDI
                     GVGRGISPMEFDAYGVDFEQAGADYEHVLNILYQGFTQERIDYRCDRFTFKDVPVVIR
                     PLQRPHPPLWYGLRGDQGPVFAARHGMHGVTLGPDEKVANMLATFRAHWRTYAADRAR
                     RQSPVETPHCGVMRAIFVADSDAEAERIARPAYKRWFDSLAWLWVERASFPPISISAD
                     YGQSKAAGTLVVGSPDTVGRILTAQAERIRQNYLVLMLAFGSLTHAQEMRSLALFKSE
                     IMPQLAAMNYEEASPAPALATA"
     gene            9447..>10455
                     /locus_tag="VM910_30785"
     CDS             9447..>10455
                     /locus_tag="VM910_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVI66930.1"
                     /translation="MASEPFDFAPLLAPSLPAPAAKWAGFPKYNFTGGNNDADHVPVD
                     RLIAAATAVLKREGSTLATYGLESGPLGYRPLREFLADKLKRTAGISCTADEILITSG
                     SLQALDLVNGALLARGDTVLIERESYQGALTRLARLGVNAVGIPLDRDGLRTDAVAAA
                     LDDLKRRGIRAKYIYTIPTVQNPTGTILSEQRRHELLRLSQDHHVPIFEDDCYADLIW
                     DGRRPPALYAMSGTGGVIHIGSFSKSIAPALRVGYIVARWDILSRILALKTDAGSGAL
                     EQMVLGEFCASHFAEHVPKLTRGLRAKLETLMEALAEQFGTSAEFDDPKGGIFLWVKL
                     PD"
BASE COUNT         2037 a         3264 c         3201 g         1953 t
ORIGIN      
        1 agcagctccc gaaactcggt accgtgggcg tttttaaatc ttgttgtaca acacgatcag
       61 gccgtatgcc ttacttggat actgccttcg ccgcgtggcc ggctgcgcta cgccgagcgg
      121 ctccgccggc cacgctagcg caacataccg tcaggagacg ttggacggca ttggcgataa
      181 gcggaaacct tatccgcgac ctaactaatc cgtgccaacc cactgcttat ttatgcccaa
      241 agccgaggac ggaaaccgaa gaaaaaacgt gctccagacc tgttggtgcg gttcacatga
      301 ccgctggcca taatggtccc gttcaaaagc ccgcattcga gatgcactgg cccttgagcc
      361 agaggatcaa ttccttcagg tctttcgcgg gcacagcttt gtttgtaaca atcagcaaag
      421 ggttggttgt gagcagcgag accggctcga aatccttcaa catgtcgtac tggagtgaat
      481 aagccgcacc ttcgcttggt cttcgacatt cggccgatct cgcgcgtcgt cgaagaggtc
      541 cgtctgcgct ataggcagac gtgacgccgc tcaagctcaa tccgcaacgt cggtgataaa
      601 tcgtactgat gccgtgcaag gtattcggtg atttcatccg cagccgttcg cggcggaccc
      661 tccatcaccg tatcgatgat ctcccgccag actgatcggt tgcgcccaac aatggccagt
      721 ggcaccacga attccgtgac cgacatgttc attgatgtgc tccccttcgg ctcccaagac
      781 gcatcaagat gcttaccgtc cttgtggtcg actgtgccgc caatcgcgga ccaaagtcct
      841 tgggacagat ctgaattgtt ctgaatcgag gtgtcggttt tggcggtcgc gattgtaaaa
      901 aacgacaact tttcaagaaa gggggggcct gtaaaagtca ttgaaatcat taggactccc
      961 caaattgctc tgaatcggcg tgtcgccgct cgctgtagcg gctcagcccg attcagaaca
     1021 attcagatct agcccaagga cctcaaggct actctggagg atgattgcag cagcccaaat
     1081 aggtcacgtc agcaatctgc ggaggcggtt gccgaatgga agggcaaagt tgacgcctcg
     1141 ctcaacggtt ggttctggca aacttgagga tagaaccatg ttaaccagcg acgaagtaca
     1201 aaagcgaaat gcctttgccg cttgtgctgc atttcgtatc gagggccttg aagcctcttt
     1261 ggatcgtgct cgcgagattg cattccaggg agagctggac gccgtagggc accacattga
     1321 agaggcacaa ggatacctgg ctcaaattcg gctcctgcat caagaagccc tagatgagtt
     1381 ctgcagagct cagggagaat aggactcttg ggcggtccgc aagcggcgat gggcgcaaag
     1441 gggggaccag tgactgtgcc gacgaggtga ccttgtctcg caaaggtgca aatagtcgaa
     1501 cgacgcgcgc ctgcaacgcc gcagatcgac gcggagctgt gcccattgat ccgccggatg
     1561 agccatcaaa atccgctgcg ggcacagcca cggccaagag ccaacgcgca tctttgcgtc
     1621 agagaaattc aggcagcggc cttaacgtga cggtgtgaac cagctcacac atgccttgga
     1681 aaaagttcgg tacaaaccaa atccgaggct gcaacaaata ttgcacccaa ccgcgggagg
     1741 ggaaccatgc ccgcaaacaa taagattcca atttgtccag cgttgacgac gaggcttctg
     1801 ctcaaaagtt catcgtcctt tggggacgac cgcaccaacg cagatcaacc taggcggtct
     1861 aagccccgtc gctcgcgaaa gggcgctttg gtttctgcaa agaaaaagct cgccctcatc
     1921 acgcgcgaac tgaaggaagc gcgacaacaa caggctgcca ccactgatgt gctcaaggtc
     1981 atcagccgct cgaccttcga tctgcaggtc gtgctcgaca cactcgtaga atcggctgtt
     2041 catctgtgcg acgctgatca tgcctggcta ttccaacgcg aaggcgagtt tttccgttgg
     2101 gttgccagct acggccatgc gaccgacgtg cacgagcgaa tcagggatta ttttcggacc
     2161 ctgccggtcc cggtggatcg aggcagtgtc actgggcgaa ccgcgctaga ggccaaagta
     2221 gttcaggttc ccgatgtgct ggcggaccca gagtatacct ggagtgaggc ccaaaagctt
     2281 ggcggctacc gagccgcgct cggcgttccg cttttgcgta aaggaaatgt cgtaggggtg
     2341 atcttcgtag cgaaaatcgt gccgcggcca tttactgcga aacagatcga gttggtgact
     2401 acctttgccg atcaggcggt catcgctatc gagaacaccc gcctactcag cgagttgcgg
     2461 caacgcaccg acgatctgag cgaatcgctg gagcagcaga ccgcggcctc ccaggtgttg
     2521 caggtaatct ccagttcgac cggcgagttg gagccggttt tcgaagccat tttggcaagc
     2581 gcgacgcgga cctgcgacgc gaaatttgga ttgctatacc ggatcgaaaa tggcgctgcc
     2641 cggattatct cgagactcgg cataccccct gcgttggcgg agtacctaaa gcgtgggccg
     2701 caccggccac cgctgaatcg gcttgatccg cacactccgg tcggccgtgt cgtccaatcc
     2761 cgtcagacag tccatgttgc cgactatcgt acagatcaag cctaccttaa ccgcgaccca
     2821 ttgacagttg caggcgttga actcggcggc atccgcacat tactcgtggt accgatgatc
     2881 aagaacgacg cgctgatcgg cgcgatcgtc atttttcgcc aggaggtccg cccgttcacc
     2941 gacaagcaga tcgacctggt tcagaatttc gccaaccaag ccgtcatcgc catcgagaac
     3001 gcgaggctac tcaacgagct ccgcgaatcg ctgcagcagc agaccaccac cgccgacgta
     3061 ctcaaggtca ttagccgctc gagtttcgat ctccaagcgg tgctcgacac attgacggtg
     3121 tcggcggcgc gacattgtga agcgcaaatg gccgcgattg tgcgccagaa ggacgcgggg
     3181 tcgtattact acgcgacgac ctacggcatt agcgccgact ccaataacta cttgaagagc
     3241 gtcgcgatta aggtaggccg agggaccgtg gtggggcggg ccttgctgga aggcaggatt
     3301 gtccaagtcc ccgatgtttt ggctgatccg gaatacacat ggactgaggc ccaggaaaga
     3361 actggctttc gctccgtgct cgcggttccg ctgctccgcg aagcacaccc gatcggcgcg
     3421 ctcgtgctgg cgcgttcagt cacaaagccg ttcaccgaca agcaggtcga ggtggtcacc
     3481 acgttcgccg atcaggcggt gatcgcgatc gagaacgtac gtctattcga tgaaattcag
     3541 gacaagagcc ggcagctagc ggaggcgagc cagcgcaagt cgctgttcct cgccagcatg
     3601 agccacgagt tgcgcacgcc gctcaatgcc atcatcgggc tgaccgaaat gatgatcaaa
     3661 aacgcggcgc gcttcggcac ggaaaaagcg caggagccgc tgcagcgagt gaaccgcgct
     3721 ggtactcacc ttcttggcct catcaaccag gtactcgacc tgtcgaagat cgaggctgga
     3781 aagctcgagc tcaacccaca gaccgtgcag ctagcgccgc tgatcaacga ggtcatcgga
     3841 accgcgggac agcttgccga gcagaacaag aatcgcctcg ccgttgacac gcaagagaac
     3901 ctcggcgcat tgaccgtgga ccccatgcgg ctgcggcaga tcctgctcaa tctcctcagc
     3961 aatgcgtgca agttcaccaa agcgggcgaa gtcaagcttg tggcacgtaa agtgagcaat
     4021 ggcagcaact tcgtcgagtt cgtcgtctcc gacaccggca tcggcatgat gcctgagcag
     4081 caggcgaagc tgttcgagga gttcagccag gccgatgccg cgaccgcgca gaagttcggg
     4141 gggacgggac tcggcctttc catcacccgc aaactcgcgc gcatgatggg tggcgacgtg
     4201 aaggtaacga gcgaggcggg caagggctcg gtgttcacgg tgcgcttgcc ggccggagaa
     4261 cgcacatgaa cgccatgaaa cttccccgcc gacaatttct gcatctggcg gccggcgccg
     4321 ccgcgttgcc ggccgtgtcg cccatcgcgc gggcacaagc ctatccggcg cggccggtgc
     4381 gggcgccgcc gcgcggcgac ttcacgatgg ccatgctcga tcagatgaac acttagtcag
     4441 gcgggcacgc cgaacaccgc gcactgaccg atgcgtcggc ggccgctcct ctccgcgctg
     4501 aatcgcgtgc tcgacgcgct caacgaaccg cgcgtccatc tgctcgagtc gctcgcgcgg
     4561 ccatgcgccc tcctgctcgt cgcccggctc gggcgaaccg atacgaagac gacgcagcgg
     4621 cgacccgcgc gagcacggtg atgaccatcg gaacagtgaa aacagagcgg cggccgaaat
     4681 gtaaactcgt agtcagggcc ccgcagctcc tcgctgtcac cgcccttgat tgatgtcgtc
     4741 gtactggcgg cgaacgtagg gctcaacagt ccctttgatt gtattggcga tgacctctgc
     4801 tgcttttgat ggctccggat gagttacata cccatggggt ttgccatagc gaaaagagtg
     4861 gtcgaggtgg tacggcgcga gcgattgcac gacttcccaa aaacgagcgt cagaaggcgt
     4921 gaacccatgt ttttgcgcgc accgaaacgc cagttccaag tcgtggccga tgtggttggc
     4981 aagtgttgtt attggcactc ccgaagcggc cagataggcc tttaacgtca actcgatcgc
     5041 gtggcaaagc agctggtaga ttggtgacac ccatttgcgg acgatgccgc cctggagcgc
     5101 ggcaagctgc cgcacgcttt cgacgtagtc ctcggcgtcc cgtaggaacc tcggagccac
     5161 gtgccgaggt ttggctattt ccatcatcag tatatgccag gcattcacct cgttagccgc
     5221 gcgccgttgc gcaggcaata gctcccgctc gggagccatc cccgtgggca tgtgccgttc
     5281 ggcgcctggc gatggcggat gatgccgtgc agaatgaacc gctcgcgatg tagccgtgcg
     5341 ggcacgagcc gcccggcccc cgtggcttcg gcacgggcaa cagctccacg accgtcgtcg
     5401 tcgtgacgag cgcgagcacg cgcgaaaagg ctgttttcat tgggtcgatc tctgtttggg
     5461 acactattgg gacactggca gcgattggcc ttgaaatcat ttcgttattt ccgccagtag
     5521 ttcgctgcgt catgggacgc aaggcgcctc gcaagcgcct ggtgtgccgc gtcataagcg
     5581 cgttcacgcg cgtctttgac gcaagatggc acgcctcacg ggtgttccgc cgagcacccg
     5641 aacgtttctc ggcgctccgc ccacccctcg attcggggtg agtgaagcga aggggcaaaa
     5701 cccggacgca aagtgcgccg cgggaacggg atgggctgtt tgacatcgtg agatgggaat
     5761 taccgaaaat ggttcggcaa cgagccgcca gctcggccgt cctcatcctg aggagcgcgc
     5821 gtgccggaag gtttgcgcaa actcgaacgg acgtgcgcgc gtctcgaagg atgaggacgg
     5881 caccgatctc gggtttaccc gagatcggca gaattaaatg cccaagtcgg ctatagccga
     5941 cttgggtggg catggccctc atgcttcgag acgcatcgca gcgcatttgg gctgtggaag
     6001 tgcccgcgct cgcgttcggc tgcgacgctc ctcagcatga gggcgacggt gcgcggcgca
     6061 ttttggcaag ccaagcggca gcgcgggcgc gaaaaaccaa cctgcgggtg taggaaatga
     6121 tcgccggccc agatccattg ttcagggatt gttatttaca atggatggcg caactccaac
     6181 gtgtcgagcc gtcagcgcgc ggcccggtcg cgcgggtggc gaggctgctg ggattccgcg
     6241 gcagcaggcg accaaagtta actcccctct tcgccgcacg acgccggatc aggatggcgg
     6301 ccacagcaga tcgatccatg catttgcaaa ccgcctggcc tcggcttgcg agacgcctga
     6361 tatgcgaaac ttgaggaact tgctttttgc tacgcccacg cacgagaaag agtcccgcgg
     6421 acggccatct tccccggtga tggcgaatgc tgtgcaaatc aatcgtgtcc ggccgtgagc
     6481 atcctcaatc gtgaagtcct ccttgacatc gactttaaca cggaccccct gcctcagacc
     6541 caggacgtct ctccgcgact gctcgagctg tgccttgacg agcggcgact gtgggtcgtc
     6601 gggaatcgat tgcttgccgt cgtcgtagat gtagacggta cctatcaggt ttccttgctg
     6661 gtagccggcg ctgtagccca gtcccggccg gatgctccgg tagtcgcgga aactgatgcg
     6721 ctgggcgccc gcgatttctt ccggaaaggt gaaatcgaaa actgtcactg gcccctttac
     6781 cgacgtttgc gcggcggcgc ccgtcactgc agaaaggaag gcgatcagaa cgacgcacac
     6841 aatgcgagtt actgctggca tctgccggtg gtcctcaacg aagtttcgtg ggatgagctt
     6901 ggccggaaca agggtaacac ttgcgatact tgacatgcgg acggcgaaca cggctcacgc
     6961 cgctccgcgc tcgcgcagca cgtcgagcgc gatgcgatgg ccctcgatcg ccgccggaat
     7021 cccgcaatag agcgcgacca gcagcagaat gtcgcgaatc tcttccgccg tgcagccgtt
     7081 ggtgagcgcg ccgttgacgt ggacgcgaag ctcgttcgcg cgcccgatcg ccgccgtcat
     7141 cgcgatcgcg gccagcgatt tggtcttgag cggcagcgcg gaccggctcc agacgtcgcc
     7201 ataggcataa gcgttgatca tgttctgcag cggctcgccg aacgcgccga acgcgttcat
     7261 gcgctgctcg accgcgtcgc ggccgaacat ctcccggcga agcttcaacc ctttgtcgtg
     7321 caggtcgcgt gtatccatcg ctctttctcc ctcaccaccc cgccggcgtt ccgaccttct
     7381 ccgcaggcgc gctcgccagc cgagccgcgt cccattcctt ggcgatgatc cggcggccgg
     7441 tgaccccgtc cgagcgcggc gacatcagcc agacgattgg cgcgatcatg acctcgggct
     7501 ggatcagggt cgagcggtcg gccacttcgg tcgccgggat catgcgcgtg ttggccggcc
     7561 cgccgggcgc gagcgcgttg acgctcacgc cggtgccggc gagatccttc gcccaaatca
     7621 cggtcgcagc ctcgagcgcg gccttcgacg gcccataggg cgagaaccct tccatcatca
     7681 tggtgtagct gctggtctcg atattgacga tgcgtcccca gccttgcgcc accaatgtcg
     7741 gggcaatggc cttggccatc aggaacggcc cgttgaagtt gacgtcgatg gcgttgcgcc
     7801 aggtgtcggc gtcgacctcg tagaattttt tgcgcgcgcc gacgagcacg ttgccgatat
     7861 gctgcatgcc gacgccggcg ttgttgacga ggccgtgcac ggcgccgaag tgctcgaccg
     7921 cgttgcgcac ggccgcggtg caatcggccc agcgcgtgac gtcgcagtcg atgggatgga
     7981 tccgctcctc tacctgttgc gcgcgggcga gctcggcgag ctcacgcatg tcggcctggc
     8041 tcgatggcag atcggcgacc gccacgcgca cgccgtcctt ggcgagacca agcgccatgg
     8101 cgcgcccgag gccgcgcgcg ccgccggtca cgatcacggc caggtcggat ggcgcagcac
     8161 ccataaagga taataccgtg ttcttcggct cgacatcaca ttgtggccgt cgatgcggtc
     8221 cgcgggaagg gcttagacgg cgtgccgcac cgatgctagg ctttgcgttc gccggcatcg
     8281 gttccggcca aaaatccggc ttaaggtcgg gggaaacgcg acatgaagtt cggcatcttc
     8341 gattacatcg acgcgcgcgg cgagacgctg cagaagacct acgaggatcg gctcgccttg
     8401 ctgcaggcgg ccgaggccgc tggcttctac ggctatcacc tcaccgagca ccacgcgaca
     8461 ccgttatcga tgacgccgtc gccgagcgtg tttctggccg ccgccgcgcg cgaaacgcgg
     8521 cgcatccgcc tcggcacgct gctctatctc ctgccgctct accacccgct gcggctgttg
     8581 gaagagctct gcatgctcga tcatctcagc ggcggccgcc tcgacatcgg cgtggggcgc
     8641 ggcatctcgc ccatggagtt cgacgcctac ggcgtcgatt tcgagcaagc gggcgccgac
     8701 tacgagcacg ttttgaatat tctctaccag ggcttcaccc aggagcgcat cgactaccgc
     8761 tgcgatcgct tcaccttcaa ggatgtgcca gtcgtgatcc ggccgctgca gcgcccgcat
     8821 ccgccgttgt ggtacgggct gcgcggcgat cagggtccgg tgtttgcggc gcgccacggc
     8881 atgcacggcg tgacgctcgg ccccgacgag aaggtcgcga acatgctggc gaccttccgc
     8941 gcgcactggc ggacctacgc ggccgaccgc gcgcgccgtc aatcgccggt cgagacgccc
     9001 cattgcggcg tgatgcgcgc gatcttcgtt gccgattccg acgccgaggc cgagcgcatc
     9061 gcgcggccgg cctataagcg ctggttcgac agcctcgcct ggctgtgggt cgagcgcgcc
     9121 tcgttcccgc cgatctcgat ctcggctgac tacggccagt cgaaggccgc gggcactttg
     9181 gtggtcggaa gccccgacac ggtcgggcgc attctcaccg cgcaggccga gcgcatccgg
     9241 cagaattatc tcgtgctgat gctggccttc ggctcgctca cgcacgcgca ggaaatgcgc
     9301 tcgctcgcgc tgttcaaaag cgagatcatg ccgcagctcg cagccatgaa ttacgaagag
     9361 gcttcacctg cgccggcgct cgcgaccgca tgacccatga cgcgacgctc gtccgcgatg
     9421 caatgagcta aagaaagaag gacggcatgg cctccgaacc gttcgacttc gcgcctctgt
     9481 tggcaccctc cttgccggcg ccggcggcca agtgggcagg gtttcccaaa tacaatttca
     9541 ccggcggcaa caacgatgcc gaccacgttc cggtcgatcg tctcattgcg gccgcgactg
     9601 cggtgctcaa gcgcgaaggc tcgacgcttg cgacctacgg cctcgagagc ggaccgctcg
     9661 gctaccgtcc gttgcgggaa tttctggcgg acaagctcaa gcgcaccgcg ggcatctcct
     9721 gcacggccga cgaaatcctg atcacgtcgg gctcgctgca ggcgctcgat ctcgtcaacg
     9781 gcgcgctgct cgcgcgcgga gacaccgtgc tcatcgagcg ggaaagctac cagggcgcgc
     9841 tgacgcggct cgcgcgcttg ggtgtcaacg ccgtgggcat cccgctcgac cgcgacggcc
     9901 tgcggaccga tgccgtggcc gccgcgctcg acgacctcaa gcgccgcggc atccgcgcca
     9961 aatacatcta caccatcccg accgtgcaga atccgaccgg cacgatcctg agcgaacagc
    10021 gccgtcacga gctgttgcgc ttgtcgcaag accaccacgt gccgattttc gaggacgatt
    10081 gctacgccga cctcatctgg gacggccggc ggccgcccgc gctctacgcc atgagcggga
    10141 ccggaggcgt catccacatc ggttcatttt ccaaatcgat cgcgccggcg ctgcgcgtcg
    10201 gctacatcgt cgcgcgctgg gacattctct cccgcattct cgcgctcaag accgacgctg
    10261 gctcaggcgc gctggagcag atggtgttgg gcgagttttg cgcgagccat ttcgccgaac
    10321 acgtgccgaa gctcacgcgc gggctgcgcg ccaagctcga gacgctgatg gaggcgctgg
    10381 ccgagcaatt cgggacgtcg gccgagttcg acgaccccaa gggcggcatc ttcttgtggg
    10441 tcaagctgcc ggacg
//
LOCUS       DATLJT010001341         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_797294,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001341 DATLJT010000000
VERSION     DATLJT010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797294"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VM910_30790"
     CDS             complement(<1..241)
                     /locus_tag="VM910_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66931.1"
                     /translation="MASFVGNFPRLIALTAVFGLLMDASLATLSADPISWRLEWPRTD
                     FSKHSVPFAGIKSGGPPKDGIPAIDAPRFERLADGK"
     gene            complement(288..746)
                     /locus_tag="VM910_30795"
     CDS             complement(288..746)
                     /locus_tag="VM910_30795"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HVI66932.1"
                     /translation="MVKWPALIAAIVVIPSLAWTGNDRMVVKKSSHSVAATLDRLSEA
                     LKTRGIGITARVDHAAAAEKVGQTLKPTQLLIFGNPKLGTPLMQSNRKIGVELPMKVL
                     AWEDDSGQVWLAYVKPEVLKSEYSVDGHDDTFREMGQALEKLADEAIKPN"
     gene            complement(778..1464)
                     /locus_tag="VM910_30800"
     CDS             complement(778..1464)
                     /locus_tag="VM910_30800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HVI66933.1"
                     /translation="MTMETEKLIERLAEVVEPVRPLARPWIRTAAWLLVAIPYIALVM
                     FVVSPRADLIAKASEWRYVIEQLAALATGITAATAAFATVIPGYDRKFLFLPALPLAI
                     WLGSLGDGCVEDWIHFGPDGLSLQPDWFCFPAIVLVGAVPAIAMAVMLRRGAPLTPHI
                     TSALGGLAAAGLGNFGLRLFHSQDASLMVLVWQVGTVFILTIMLAWAGQYLLNWRSIV
                     ATARRRVLIR"
     gene            complement(1461..2027)
                     /locus_tag="VM910_30805"
     CDS             complement(1461..2027)
                     /locus_tag="VM910_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI66934.1"
                     /translation="MKGQLDDHALATLMRAAQGGNTQAYVLLLKEITPRLRQIVRAQR
                     RFLKIADIEDLVQDVLLSLHAVRATYDPRRPFMPWLMAITRHRLADAARRHTRRAAHE
                     VQVENVPVTFSDEGTNIDGDEYRDPEALRQAIEELSPAQRAAIEMLKLREMSLKEAAA
                     ARGTSIGALKVSVHRAMATLRKALIKES"
     gene            2170..2354
                     /locus_tag="VM910_30810"
                     /pseudo
     CDS             2170..2354
                     /locus_tag="VM910_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848063.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            3003..3215
                     /locus_tag="VM910_30815"
     CDS             3003..3215
                     /locus_tag="VM910_30815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66935.1"
                     /translation="MSTDTMIAVAAALVLVAGTAAVAMINYEANAPLAAFAPQNDPAA
                     VSAQNPVAGCDTDEAPIFQGQWPLHY"
     gene            3407..>3636
                     /locus_tag="VM910_30820"
     CDS             3407..>3636
                     /locus_tag="VM910_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVI66936.1"
                     /translation="MHSKIRTTLTLRALHVLLGLTLCALAAAVLLAFSVVGHAAQPFD
                     DQSFKQAQAAGKTILVDVAAPWCPTCRRQRPIV"
BASE COUNT          765 a         1120 c         1013 g          738 t
ORIGIN      
        1 ccttgccgtc cgccagccgc tcgaaacgtg gcgcatcaat cgccggaatt ccatccttcg
       61 gagggccacc ggatttgatc ccggcgaacg gcacgctgtg cttggagaaa tccgtgcgcg
      121 gccattccaa acgccagctg atcggatcag ccgacaaggt tgcgagactg gcgtccatca
      181 acaggccaaa gaccgcggtt agcgcaatca atcttgggaa attgccgaca aacgacgcca
      241 ttgcaatctc acgccatctt tcggtcgacc cacagagctg cccgaggtca gttcggcttg
      301 atcgcttcgt cggccaactt ctcgagcgcc tgacccattt ccctgaatgt gtcgtcgtga
      361 ccatcgaccg aatattccga cttgagaact tccggcttga cataggccaa ccacacctgc
      421 ccgctgtcgt cctcccacgc gagcaccttc atcggcagtt cgactccgat ctttcgattc
      481 gattgcatca agggggtacc caactttggg tttccgaata tcaagagttg ggtgggcttg
      541 agtgtctggc caactttctc tgcggctgcc gcatggtcaa ctcgagctgt aatcccgatg
      601 cctcttgtct tgagcgcctc ggatagccga tccagtgtcg ccgcgaccga gtgggagctc
      661 ttctttacga ccatgcggtc gttgccggtc catgcaagcg agggaataac gacgatggca
      721 gcaatcagcg cgggccattt gaccatgatg gcactccttg tcgtattttg atttggatta
      781 tcggattagc accctcctgc gagcggtggc gacgatcgaa cgccaattca gcaggtattg
      841 ccctgcccat gcgagcatga tcgtcaatat gaatacggtt ccgacctgcc aaacgagaac
      901 catcaggctc gcgtcctggg aatgaaacag ccgcagcccg aaattaccga gtccggctgc
      961 cgccaggccg ccgagggcgg acgttatgtg cggggtaaga ggtgcgccgc ggcggagcat
     1021 aacggccata gcgattgccg gcactgcgcc gacgagaacg atcgcaggga agcagaacca
     1081 atccggctgc agcgataagc catcaggacc aaagtgtatc caatcttcga cacagccgtc
     1141 gcccaagctt cccaaccaaa ttgcgagcgg caacgccgga aggaagagaa atttgcgatc
     1201 gtaccctggg atgaccgtag cgaaagccgc agtcgccgca gtgattccgg tggcaagcgc
     1261 agcgagctgc tcgatcacat agcgccattc cgacgccttc gcgatcaagt ccgcacgcgg
     1321 cgaaacgacg aacatgacga gcgcgatata agggatggca accaatagcc aggctgccgt
     1381 tctgatccat ggacgcgcaa gcggccgaac aggctcaaca acttccgcca accgctcgat
     1441 gagtttctcg gtctccatgg tcatgattct ttgatcaagg ccttgcgaag ggtagccatt
     1501 gcacgatgaa ccgaaacctt aagcgccccg atgctcgtcc ccctcgcagc ggcggcttct
     1561 ttcagcgaca tttcgcgtaa tttcaacatc tcgatggcgg cccgctgcgc gggagaaagc
     1621 tcctcgatcg cctgcctcag cgcctccgga tcacggtatt catcaccatc tatgttcgtt
     1681 ccttcatcgg aaaaggttac aggcacattc tcaacctgta cctcgtgggc tgctcgccgg
     1741 gtatgccgcc gcgccgcatc ggccaaccga tgccgggtaa tcgccatgag ccaaggcatg
     1801 aacgggcgtc gcgggtcgta ggtggcccgg accgcatgca gagatagcaa tacatcctgc
     1861 acaagatctt caatgtccgc aatcttcaga aatctgcgtt gtgcgcgaac gatctgccgc
     1921 aagcgaggcg tgatctcttt cagcaataga acataggctt gcgtgttgcc tccctgtgcg
     1981 gcccgcatta gcgtcgcaag agcgtgatca tccagttgcc ccttcattgc caattaatac
     2041 gtccgatcgt cgcggtgggc cgaaacactt cgtcgtgaat ggggcgactc tacttctcca
     2101 ttcaccagcg cgaattcagt tgaatgtgag tcatgacgct caccccgaag taaaaaggca
     2161 cgcgcaatca ttcgcaggtg tctggagcga ttgtcaccct catcgtgaag tgatgaatct
     2221 cgtctattac caagacaatc gaagaggtag ccgagatcgt tggcgctccg acaaacaccg
     2281 tcaagacgcg catgttctac gcgcgtcacc aactggcgga gctgcttcgt gaagcgggcg
     2341 tggatgggct ttgattgtgc agcgcgaaag gcctcggaca cctattcgtg attcgatgga
     2401 aggtaaccgt ttctctccgc aactcgaact cgatagcggg tgagcaaatc tgctcactgt
     2461 agatgcgtta aatggagaga caccatgaaa acgctaatga ctgctgtcgc gcttgctacg
     2521 gtgtttgctg caccggcttt cgcgcagtcc gtgaacgcct ggggatggaa acgagcggtc
     2581 gccgctccgg ccagcgccaa gtccgcgtac gccgccgcgt cgttcaacgc tcgccgcggc
     2641 gctctgaccg gtcgccgcag cgctctgacc ggccgccgcg gcgctccggc gggttggacc
     2701 tacgctcgta actgcaacat ttccccggcg agcccgtcgt accggactaa ctgcaactag
     2761 cgtcgatcgg caacccagcg agagttcctg aatggggctt actccacggg gtactttgcc
     2821 gtcgatgcgg ccggcgttcg cgctcggccg caacttcgtt gggcggccgc aagctctcgt
     2881 tggtgcaaac ttggcaacgg gtcgagcacg tcgtcgtgaa ttagacgaac gtaacctctc
     2941 tgctcattta cgcgaactta aattgcaggt gacgcgctca tctcgaacta ggagccacga
     3001 cgatgtctac cgacactatg atcgcagttg ccgcagcgct cgtccttgtc gcaggtacag
     3061 cggcggtggc gatgatcaat tacgaagcca acgcccccct tgcggcgttc gccccgcaaa
     3121 acgatcccgc cgcggtctct gcccaaaacc cggtggcagg ctgcgacacc gatgaagcac
     3181 cgatttttca aggacaatgg ccgcttcatt actgacacga gcctgccttc gctaaagcag
     3241 caatcacgct gctagctgtg cgcccatcac ataactcatc ggcggggttt tgaggcctta
     3301 ggccggaata tttcacatcg aacgtaacct tttccgaccc tgcgacgaac gtagtgtcac
     3361 gcgggccgtt atggcccgac ggggatgggg cggaaacaag cagaccatgc attccaaaat
     3421 tcggacaacg ctgaccctgc gtgccttgca tgtcctgctc gggctgactc tgtgtgcgct
     3481 ggccgcagcg gtcctgctcg ccttctcagt agtgggccac gctgcgcaac cattcgacga
     3541 ccagtccttc aagcaggccc aggcagccgg caaaacgatc ctcgtcgatg tagctgcccc
     3601 ctggtgtccg acctgcaggc ggcagcgccc gatcgt
//
LOCUS       DATLJT010001342         1827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3326380,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001342 DATLJT010000000
VERSION     DATLJT010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3326380"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VM910_30825"
     CDS             complement(<1..402)
                     /locus_tag="VM910_30825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66937.1"
                     /translation="MNHIDGRMAGALLMMVSLGACVMPPTTSTIPVAPGPNKTFEVFA
                     AEQSYCQQYATAQTAPQAYAATNRAVATAVLTTALGAALGGAIGGGHAAGIGAASGAA
                     FGTVVGAGGSGFAQMSLQQQFDITYGQCMYAH"
     gene            579..1532
                     /locus_tag="VM910_30830"
     CDS             579..1532
                     /locus_tag="VM910_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI66938.1"
                     /translation="MDVETKADRFVGIDVSKARVDVHVRPDGIAFGCATDPEGLGALG
                     ARLKPLLPCLVVLEASGGYEGAVAAALVEAGLPVAIVNPRQVRKFAGAIGRLAKTDAI
                     DAAVIAHFAQAVRPPARPLPDDLSVRLAELMARRRQLVVMINAEKQRLAKARDPIAQS
                     SFKAVLKRLQAERARIDKAIDRLIEVSPLWCAKQDLLKSVPGIGDVVARTLIAELPEL
                     GRVDRHQIAALAGVAPINRDSGRYRGKRKIQGGRVEVRAPLYMACLVAIQHNPVLKSF
                     YRRLREAGKPARLALVAAMRKLLILLNAILRDQRPWAHAQT"
BASE COUNT          326 a          592 c          596 g          313 t
ORIGIN      
        1 gtgcgcgtac atacattgcc cgtacgtgat atcgaattgc tgctgcaggg acatttgtgc
       61 aaaacccgaa ccgcctgcgc caactaccgt accgaacgcc gcccccgacg cagcgccgat
      121 cccagcggcg tgaccgccac cgatagcacc acccagggca gcgccgagtg ccgttgtgag
      181 aaccgcggtc gcgacggctc ggttggtcgc agcataggcc tggggtgcgg tctgcgcggt
      241 ggcatattgc tggcaatacg attgttcggc agcgaagact tcgaatgtct tgttcggccc
      301 tggggcgacc gggatggttg atgtcgtggg cggcatgacg cacgcgccga gagagaccat
      361 catcagcaga gcgccagcca ttcgcccatc gatgtgattc atcctgcctc ctatctcggc
      421 attgcttggt ttgttgagct acgctggcta accgagcccg gccgatagcc cgaatggttc
      481 gaggtctttg aggccgcagg ggagcgtgtg gcgccggccg gcatccgatg aacccgaaca
      541 gtcgtctggg cacgagcccg catcgaggag gctgggtgat ggatgtcgag accaaggccg
      601 atcgctttgt cggcatcgac gtatcgaagg cgcgggtcga tgtgcatgtc aggccggatg
      661 gcattgcgtt cggctgtgct acggacccag aaggactggg cgcattgggc gcacgattga
      721 aaccgctgtt gccctgtctg gtggtgctcg aggcgagcgg cggctatgaa ggcgcggttg
      781 ccgctgcgct ggttgaagcc gggctgccgg tggccatcgt caatccgcgc caggtgcgca
      841 aattcgccgg ggctattgga cgattggcca agaccgacgc gatcgacgcc gcggtgattg
      901 cccacttcgc ccaagctgtg cgcccaccgg cacggccact gcccgatgat ctctcggtgc
      961 ggctcgccga attgatggcg cggcggcgcc agctggtggt catgatcaat gccgaaaagc
     1021 agcggctcgc caaagctcgg gacccgattg cgcaaagctc gttcaaggcc gtgctcaagc
     1081 gcctccaagc cgaacgggcg cgcatcgaca aggcgatcga caggctgatc gaagtcagcc
     1141 ccctgtggtg cgccaaacag gatctgctta aaagcgtgcc cggcatcggc gatgtcgtcg
     1201 ctcgtactct gatcgccgaa ctgcccgagc tcggccgggt cgatcgccac cagatcgccg
     1261 ccctggccgg cgtcgccccg ataaaccggg atagcggccg ataccgcggc aagcgcaaga
     1321 tccagggcgg ccgcgtcgag gtccgagccc cgctctacat ggcctgcctt gtcgccatcc
     1381 agcacaaccc ggtgctcaag tccttctacc gccgtctgcg cgaggccggc aaacccgcac
     1441 gcctcgccct cgtcgctgcc atgcgcaagc tgctgatcct tctcaatgcc atcctgcgcg
     1501 atcaacggcc ctgggcacat gcccagacct gagcgtcgaa gccatggctg ccactccggc
     1561 tgcggcgagc gccggctcaa ggccggccgc cgccggaggc gggagcgcga cagcgttcgc
     1621 cttgaaccgg tgttcgacgt ggccgaccct tcagccatca cttcgacacc tgaccttcaa
     1681 gacagtcgct cccctgagga ggagacggag tacgcgtgca ccatgggccc tgccgctcgg
     1741 ctgatcgaag agaagaagcc ggacgaggct atgcgcgcga cgatcaggcg agagatgatc
     1801 gaggcgtttg cggtctatgc ccaagcc
//
LOCUS       DATLJT010001343         2454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1260328,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001343 DATLJT010000000
VERSION     DATLJT010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260328"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VM910_30835"
     CDS             <1..847
                     /locus_tag="VM910_30835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI66939.1"
                     /translation="AQRTRRLRFGALVYVLPAHHPLRLAEEICMLDHLSGGRVEVGIG
                     RGASPHELAYWGIDPDGAQATYVEAYHVIMQALTRDAVNFAGKHYRFENVPIELKPLQ
                     RPYPPLWYAVPVPEGADWPAQNRINIVCGGPLPRVREITERYRAAWAAAGHSSEQPPL
                     LGITRFVVADDSDHAAMALGRRAWPVFYRSFMKLWKLHGTQPRYARIPEDFDTLVDYG
                     GAIAGSPATIRERVRHMTEEAGANYFISQFSFGDLSQEEVLHSVSLFAREMIPGAHER
                     VARAV"
     gene            996..2204
                     /locus_tag="VM910_30840"
     CDS             996..2204
                     /locus_tag="VM910_30840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI66940.1"
                     /translation="MTDTAPNGPLAGLRVLDISTVVAGPFASTLLADLGAEVLKVEMP
                     GSGDALRRLAPHKDGIPLWWKVTNRNKRGITLDLRKPEGKALLGRLIESYDVLVENFR
                     TGTLDDWGITRDWLQATNPRLVILRVTGFGQTGPYRNNPGFARVFEAMSGLTRMCGEE
                     GGTPLHLGYPISDAIGGLFGAIGILAELYRLKDDKTARGQEIDCAVTEAMMRTLDFLA
                     IEYDQLGSVRTQTGNRSQYAAPGNIYGTSDGKWASIAASTQSIFERFCKALGMPHLIT
                     DPRFIDNPSRVKNWQAIDRIVADAISKLTLDELRALLHKYEVGFSPIYDAADIFADPH
                     FIARESIVSVADEELGPVRMQCVVPRFSETPVAVRRAGPALGQHNDEIFAALGLSASD
                     IARLKANGTI"
     gene            2215..>2454
                     /locus_tag="VM910_30845"
     CDS             2215..>2454
                     /locus_tag="VM910_30845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI66941.1"
                     /translation="MPRALVAVVALALMFADARAAELTRILVGFPPGQATDLIARILA
                     ERLAPMLGETVIVENRVGQGGSIALAALAQAPSDGH"
BASE COUNT          424 a          779 c          791 g          460 t
ORIGIN      
        1 tgcgcaacgc accagacgcc ttcgcttcgg cgccctggtc tatgtgctgc cggcgcatca
       61 cccgctgcgg ctcgccgaag aaatctgcat gctcgatcat ttgagcggag gccgcgtcga
      121 ggtcggcatc ggccgcggcg cctcgccgca cgaactggct tattggggaa tcgatcctga
      181 cggggcgcag gcaacctatg tcgaggccta tcacgtcatc atgcaggcgc tgacccgcga
      241 tgcggtgaat tttgccggca agcattatcg cttcgagaac gtgccgatcg agctgaagcc
      301 gctacagcgt ccgtatccgc cgctctggta tgcggtcccc gtgccggaag gcgcggattg
      361 gccggcgcag aaccgcatca atatcgtgtg cggcgggccg ttgccgcgcg tgcgcgagat
      421 caccgagcgt tatcgtgctg catgggccgc cgccggacat tcgtccgaac aaccgccgct
      481 gctcggcatc acccgctttg tggtagctga tgacagcgac catgcggcga tggcgctcgg
      541 ccgccgcgcc tggccggtgt tttaccgaag cttcatgaag ctctggaagc tgcacggtac
      601 ccagccgcgc tatgcccgca ttcccgaaga tttcgacacg ctggtcgatt acgggggtgc
      661 gatcgcagga tcccccgcca ccattcgcga gcgcgtgcgt cacatgacgg aagaggccgg
      721 cgcaaactat ttcatcagcc agttttcgtt cggcgatctc agccaggagg aagtgctgca
      781 ttcggtcagc ctgttcgccc gcgagatgat accgggggca catgaacgcg tggcgcgcgc
      841 cgtctgatgc agccgaccag tgacgggtct acttctctgc gcgctggcgt gctgcgagtt
      901 gagcatcagc cgatcccggg ttatgccgat acggtaaatc tgatttatct gatttgacca
      961 tatcgcatag gcgccgggac cggtagcatc ggcccatgac cgataccgcc cccaacgggc
     1021 cgctcgccgg cctgcgtgtc ctcgacattt ccaccgtggt cgccggcccc tttgcctcga
     1081 ccctgcttgc cgacctcggc gccgaggtct tgaaggtcga aatgcccgga agcggcgatg
     1141 cgctgcgccg gctcgccccg cacaaggacg gcattcccct gtggtggaag gtgacgaacc
     1201 gcaacaagcg cgggatcacg ctggatttgc gaaagccgga aggaaaagcg ctgctcggcc
     1261 ggctcatcga gagctacgac gtgctggtgg aaaatttccg taccggcacg ttggacgact
     1321 ggggcatcac gcgcgactgg ttgcaggcaa ccaacccgcg gcttgtcatc ctgcgcgtca
     1381 ccggttttgg ccagaccgga ccctaccgca acaatcccgg ctttgcccgc gtgttcgagg
     1441 cgatgagcgg gctgacgcgc atgtgcgggg aagagggcgg cacgccgctg catctcggct
     1501 atcccatttc ggacgcgatc ggcggactgt tcggtgcgat cggcattctg gccgagctct
     1561 atcgtctgaa ggacgacaag actgcacgcg ggcaggagat cgattgtgcg gtgacggaag
     1621 cgatgatgcg cacgctcgat ttcctcgcca tcgaatacga ccagctcggc tcggtgcgca
     1681 cccagaccgg caaccgcagc caatatgccg cgcccggcaa tatctacggg acatccgacg
     1741 gcaagtgggc ctcgatcgcc gcgtcaacgc aaagcatctt cgagcgcttt tgcaaggcgc
     1801 tcggcatgcc gcatttgatc accgatccgc ggttcatcga caatccctcg cgggtgaaga
     1861 attggcaggc aatcgatcgc atcgtggccg acgcaatttc gaagctgacg ctggatgagt
     1921 tgcgtgcatt gctgcacaag tacgaggtcg gcttttcgcc gatctatgac gccgccgaca
     1981 tctttgccga tccgcatttc atcgcgcgcg agtcgatcgt cagcgtcgcc gatgaggagc
     2041 ttggccccgt taggatgcaa tgcgtggtcc cgcgcttctc cgagaccccg gtcgcagtgc
     2101 gtcgggccgg gccagcgctc ggccagcaca atgatgaaat ttttgccgcg ctcgggttat
     2161 cggcgtcgga tattgcgcgg ctgaaagcca acggtacgat ctagggaggt gcgcatgccg
     2221 cgagcgcttg tcgctgtcgt cgctcttgcg ttgatgttcg cggacgcgcg ggccgccgag
     2281 ttgacgcgca tcctggtcgg cttcccgccg ggacaggcga ccgacctcat cgcgcgcatc
     2341 cttgccgaac ggcttgcgcc gatgctgggc gagacggtga tcgttgaaaa ccgcgtgggg
     2401 cagggcggca gcatcgcgct tgcggcgctg gcgcaggcgc cgagcgacgg ccac
//
LOCUS       DATLJT010001344         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_797725,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001344 DATLJT010000000
VERSION     DATLJT010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797725"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..751)
                     /locus_tag="VM910_30850"
     CDS             complement(17..751)
                     /locus_tag="VM910_30850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66942.1"
                     /translation="MLRLENLSCGYGAIRAVEDLSIDVPAGSALALLGPNGAGKTSTI
                     MAIMGQTTIHAGRILFEGSDITQKPAVTRGALGIALVPEGRRLFSELTVEENLTVGGY
                     CRSVGHDSINRERVYDLFPRLYERRKQLAESMSGGEQQMLAIGRALMAEPKLLLIDEL
                     SLGLMPKMVDLCFQAIEQLRRGGLTLVLVEQSTARALEIAESVCVLVSGSTVFFGPAA
                     IARADTALFESFLSPRPHDPMAQQER"
     gene            complement(738..1502)
                     /locus_tag="VM910_30855"
     CDS             complement(738..1502)
                     /locus_tag="VM910_30855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI66943.1"
                     /translation="MTAALEVRAVAIRFGGVQAVAGVSCSIARGDLVGLIGPNGAGKT
                     TLLKIIAGVLAPDVGRVMIAGADVTDLPTELRVRKGLAITNQIVRPFRSMTVLDNVTL
                     AAGHRLTGNPFGALTRYRRDAQEVRAREILEEVGLAGEGRKWVSELPLGHLKRLEVAR
                     ALAVDPILVVLDEPLADLNQREAAQQADVIVAINAKGITTVLIEHNLGEVMRVSRRLL
                     VLDQGRLIADGAPDAVIADTRVREAYLGNAQNHVAA"
     gene            complement(1499..2329)
                     /locus_tag="VM910_30860"
     CDS             complement(1499..2329)
                     /locus_tag="VM910_30860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI66944.1"
                     /translation="MSAYVTSVLAVIGINVMLAVSLNMITGFCGQFSLGHAAFYGAGA
                     YGAAVLAAADASPPLCLLAGILVAGLLGLIVGFASLRLRHDFLAVTTIGVSFLFVGFV
                     RKQSWLGAEMGISKIPPSGLGPLGNTLLIVACAVATIALSLYVNRSWMGFGFRAVGDD
                     EDTARTVGIPVSNYKLAAFTLGTGIAGLAGGLYAYFTQFGFIVSVALMAMVVIGGSGS
                     TLGVSAAAIILTLLPEMFRFVNEYRLLIFGGLLVVVMRFAPGGLAGVARAILVKARGP
                     "
BASE COUNT          502 a          790 c          737 g          385 t
ORIGIN      
        1 aaatcgaagt tgctcatcac ctctcctgct gcgccatagg atcgtgcggc cgggggctca
       61 gaaagctctc gaacaaggcc gtatccgcac gcgcgatcgc ggctggcccg aagaataccg
      121 tcgagccgga gaccagaacg cacacgcttt cggcaatttc gagggcacgc gccgtgctct
      181 gctccacaag cacaagcgtc agcccgcccc gccgtagctg ctcgatcgct tggaagcaca
      241 gatccaccat cttcggcatt agacccagtg aaagctcatc gatcaacagg agcttcggct
      301 ccgccatgag cgctcggccg atcgcgagca tctgttgctc gccgcccgac atcgactcag
      361 ccaattgctt gcggcgctcg tacagccgcg ggaataggtc gtacacccgc tcgcgattga
      421 tgctgtcgtg ccccacactg cggcagtagc cgcccactgt gaggttctct tcgactgtaa
      481 gctccgagaa cagcctgcgc ccctcgggga ccagtgcgat gccaagtgct ccccgcgtca
      541 ccgccggttt ttgggtgatg tcgcttccct cgaacaagat tcgtccggcg tggatggtcg
      601 tctggcccat gatcgccatg atggtggagg tcttgccggc gccgttgggg ccgagcaagg
      661 ccagcgccga accagcgggc acgtcgatgc tcaggtcctc gaccgcgcgg atcgcgccgt
      721 atccacagct gaggttctca agccgcaaca tggttttgcg cattgcccag ataggcctcg
      781 cgcacgcgcg tgtccgcgat gacggcgtca ggcgcgccgt cggcgatgag cctaccctgg
      841 tcgaggacga ggagccgacg gctcacacgc atgacttcgc ccagattgtg ctcgatcagc
      901 accgtcgtga tgcctttggc attgatggcg acgatgacat ccgcctgttg cgcagcttcg
      961 cgttgattca agtccgccag cggctcgtcc aaaacaacga ggatcggatc gaccgccagc
     1021 gcacgtgcga cttcgagccg cttgaggtga ccaagcggca gttccgagac ccacttgcgt
     1081 ccctcgcccg caagcccgac ctcttcgaga atctcgcgcg cgcgtacctc ctgcgcgtcg
     1141 cgtcgataac gcgtcagggc gccgaaggga ttgccagtaa ggcgatggcc ggcggcgagc
     1201 gtcacgttgt ccaatacggt catggagcgg aacgggcgca cgatctggtt ggtgatcgcc
     1261 aaacctttgc gcacgcgcag ctcggtcggc aagtccgtca catcggcccc cgcgatcatg
     1321 acccgaccca cgtccggcgc aagcacaccg gcgatgatct tgagcagcgt ggtcttgccg
     1381 gcgccattcg ggccgatcag gccgaccagg tcgccgcggg caatcgagca gctcaccccg
     1441 gcaacagcct ggacgccgcc gaagcgaatc gcgacagccc tgacttccag tgccgccgtc
     1501 atggcccgcg cgccttcacg agaatggcgc gggcaacacc cgcgagtccg cccggcgcaa
     1561 agcgcatgac gacgacaaga aggccgccga agatcaacag ccgatattcg ttgacgaagc
     1621 ggaacatttc gggaagcagc gtgaggatga tcgccgcggc gctcacgccc aaagtggatc
     1681 cactgccgcc gatcacgacc atcgccatga gagcaacgga aacgatgaag ccaaactggg
     1741 tgaaatacgc ataaagtccg ccggcgagtc cggcgatccc cgtgccgagc gtgaaggctg
     1801 cgagcttgta gttggacacg ggaataccga cggtgcgggc ggtatcctcg tcatctccga
     1861 ccgcccggaa gccgaaaccc atccagctgc gattgacata gagactcagc gcgatggtgg
     1921 cgacggcgca tgcaacgatc aacagggtat tgcccagcgg cccaagacct gacggcggaa
     1981 tcttgctgat tcccatctct gctcccagcc atgattgctt acgcacgaag ccgacgaaca
     2041 gaaagctcac gccgatggtg gtcaccgcca aaaagtcgtg acgcaagcgg agtgaggcaa
     2101 agccgacgat gagaccgagc agaccggcga cgaggatccc ggcaagcaga caaagcggag
     2161 gtgaggcgtc ggcagccgct aaaacagcgg cgccataggc gccggcgccg taaaaggccg
     2221 cgtgccccag gctgaactgc ccgcaaaaac ccgtgatcat gttcaggctc acggccagca
     2281 tgacattgat gccgatgacg gcaaggacgc tggtgacata ggcgctcatg ccgctttcat
     2341 tccgccaatg ccttgcggcc gggcgagcag cacggcgatc aggaacagaa acgcaatagc
     2401 gtcacgatcc agaa
//
LOCUS       DATLJT010001345         3660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_340546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001345 DATLJT010000000
VERSION     DATLJT010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..680
                     /locus_tag="VM910_30865"
     CDS             243..680
                     /locus_tag="VM910_30865"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HVI66945.1"
                     /translation="MTGFRTLLFCAVSVVAASASAENLIEPGQWKVTGMTAVNGAVSP
                     PQAKARCVTPEQAEDVAKTFGPVSGTINSTCEAPAVEIADKTLKWRLVCRGQLDMEVT
                     GAFNFDTPRHYTATITSQGRMAGALISDVKTQLEGERIGECQQ"
     gene            complement(714..2963)
                     /locus_tag="VM910_30870"
     CDS             complement(714..2963)
                     /locus_tag="VM910_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVI66946.1"
                     /translation="MPPAHRAPLLRSTGLSTVLSIILATSLTVALGWSTSAAPAPEKP
                     KIPLPKPRPIARNVVPKATLAVATTTAARQAPPAGPQVSSSLAGPAPPARQRVTAPTP
                     ARKPVTASAVAATTATSQADTEALENVIELIRRHRPDDASQVEATISDPVARKLAEWI
                     ILRSDNNNATVERYSAFLTTNPSWPSQSFLRKRLEAALWDDKREDQAVWSWFENESPL
                     TAKGRFALAKVMLARGDRANAERLVREAWRNDPMSEDTESIALEMFGPLVTAGDQKAR
                     MDLFLYTTDNEAAAMRAAKRLGSGYVALAKARLAAVGRASNAKALLEAVPHELRGDVG
                     YLFSRIQLLRRDEKFAEAAQLMLSAPRDPAHLHNLDEWWVERRLLSRKMIDAGEYRTA
                     YQIARDAALPTRDIYKTEQEFTAGWIALRFLNDPGLASQHFARIGVGSLNPTTLARAG
                     YWQGRAAEAAGRSQEARAAYAAAAEQSTSYYGQLARARLGLPQLELKEAPRTRGAERL
                     EIVRAVQLLFDLDERDIAIPIFADVGENGDADALAGLGEIAARHTYARGQLLVGKAAL
                     NRGLPFDLHAYPVNGIPRYQSIGPEVEHSVVFAIARQESAFNPGDVSPAQAYGLMQVT
                     PDAGRYVCKRAGIAFDLSRMKTDPAYNVTLGAAELGGLLEDYRGSYVMTFAAYNAGRG
                     SLKKWVDRYGDPRDPKVDAVDWVELIPFAETRNYVQRVMENLQVYRARFGGGARLQIE
                     ADLHRGKVE"
     gene            3218..>3660
                     /locus_tag="VM910_30875"
     CDS             3218..>3660
                     /locus_tag="VM910_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI66947.1"
                     /translation="MAAKTKFRGSFTALVTPFKNGSLDEAAFRALVNWQISEGTHGLV
                     PVGTTGESPTLSHDEHKQVVEWCIDEAKGRVPVIAGAGSNSTKEAIELAEHAEKAGAN
                     AVLVVTPYYNKPTQEGLYQHFKAINDAIGIPIIIYNIPPRSVIDMS"
BASE COUNT          662 a         1123 c         1198 g          677 t
ORIGIN      
        1 ccatgcttgc gttccgcgaa cccttccaat gtaaccccca atgtaacctt cgagtggcgc
       61 tttgggtagc ggaggatggg gagcggtgga gtacaatcgt tgtaaatgtt atagatttta
      121 cgctgacatt cacgctatcg gaggatgata gaggaaggta cggcggactc cgtctccgcc
      181 acccgcctct gccagcgact gtccgccgcg caatagaaac agctttctca agggatcggc
      241 gcatgactgg gttcaggaca ttgttgtttt gcgcggtttc ggtggtggcg gcgtctgctt
      301 cagccgaaaa cctgatcgag cccgggcaat ggaaggtgac cgggatgacg gcggtaaatg
      361 gcgccgtttc gcccccgcag gcgaaggcgc gctgcgtcac gcctgagcag gcagaggacg
      421 tcgccaagac gttcgggccc gtgtcgggca ccatcaactc gacctgcgag gcccctgcag
      481 tcgaaatcgc cgacaaaact ttgaaatggc gccttgtgtg cagggggcag ttggatatgg
      541 aggttacggg cgccttcaac ttcgatacgc cgcgccacta cacggcaacc atcacctcgc
      601 agggtcggat ggcgggcgcg ttgatctcgg acgtcaagac gcaattggaa ggcgagcgca
      661 tcggcgaatg tcagcagtga ggggatgaag cggcgaaagg agagggacgc tggctattcc
      721 actttgccgc gatgcagatc ggcttcgatt tgcaggcgcg cgccgccgcc aaaccgcgca
      781 cggtagacct gcaggttctc catcactcgc tgtacgtagt tgcgagtctc cgcgaacgga
      841 atgagctcca cccaatcgac cgcatcgacc ttgggatcgc gcggatcgcc gtagcggtcg
      901 acccatttct tcaggctgcc gcggccggca ttgtaggccg caaacgtcat gacgtacgag
      961 ccgcggtaat cctcgagcaa gccgccaagc tcggctgcgc caagcgtcac attgtaagcc
     1021 ggatccgtct tcattcgcga caggtcgaag gcaatgccgg cccgcttgca gacatagcga
     1081 ccggcatccg gcgtcacctg catcagcccg taggcctgcg ccggcgagac gtcgcccgga
     1141 ttgaatgcgc tctcctgtcg cgcgatggca aaaacgacgc tgtgctcgac ctccggcccg
     1201 atcgattggt agcgcggaat gccgttcacc ggataggcgt gcagatcgaa cggcaatccg
     1261 cgattgagcg cagccttgcc gaccaggagc tggccgcggg cataagtgtg gcgcgcggcg
     1321 atttcgccga gacctgccag cgcgtcggcg tcgccgttct cgccaacgtc ggcgaagatc
     1381 gggatcgcga tgtcgcgctc atcgagatcg aacaggagct gcaccgcgcg cacgatctcc
     1441 agccgctcgg ccccccgcgt ccgcggcgct tccttcaact cgagctgagg caggccgagc
     1501 ctggcgcgcg caagctgacc gtaatagctg gtcgattgct cggcggccgc tgcatatgcc
     1561 gcccgggcct cctgggaacg accagccgct tccgcggcgc gtccctgcca ataaccggcg
     1621 cgcgcgagcg tggtcggatt gaggctgccg acgccgattc gcgcgaagtg ctgcgaagcc
     1681 aacccaggat cattgaggaa gcgcaacgcg atccagcctg cggtgaattc ctgctcggtc
     1741 ttgtagatgt cgcgcgtggg aagcgcagcg tcgcgtgcga tctgataggc cgtgcggtac
     1801 tcgccggcgt cgatcatctt gcgcgacaat aaccgccgct cgacccacca ttcgtcgaga
     1861 ttgtgcaggt gcgccgggtc tcttggcgcg ctgagcatga gttgagccgc ctcggcaaac
     1921 ttctcgtcgc ggcggagcaa ttgaatcctg ctgaagaggt agccgacatc gccacgcagc
     1981 tcgtgcggaa ccgcctcgag cagggctttc gcattcgacg ccctcccgac ggcggcaagc
     2041 cgcgcctttg ccagggcgac atagcctgag ccgagccgtt ttgccgcgcg catggcggca
     2101 gcttcgttat ccgtggtgta caggaatagg tccatccgcg ctttctgatc gcctgccgtc
     2161 accagcgggc cgaacatctc gagcgcgatg ctctcggtat cctcggacat cggatcgttg
     2221 cgccaggctt cccgcaccag gcgttcggcg ttggcgcggt cgccgcgcgc gagcatgact
     2281 ttcgccagcg cgaagcggcc ctttgcggtc agcggtgatt cgttctcgaa ccacgaccag
     2341 acggcctgat cctcgcgctt gtcgtcccac agcgccgcct ccagccgctt tcgcaggaat
     2401 gattgcgagg gccagctcgg attggttgtc aggaaagcgc tgtagcgctc gacggtcgcg
     2461 ttattgttgt cgctgcgcag gatgatccat tcggcgagct tcctggcgac gggatcggag
     2521 atcgtcgctt cgacctggct cgcatcgtca ggtctgtgcc ttcggatcaa ctcgatgacg
     2581 ttttcgagcg cttcggtatc ggcctgcgac gtggcggtcg tcgccgcgac cgcggaggcc
     2641 gtgaccggtt tgcgtgcagg ggtcggcgcc gtgacgcgct gacgggccgg cggcgcgggt
     2701 ccggcgagcg aggaagaaac ctgcggacca gccggtggcg cttgccgggc tgcggtggtc
     2761 gttgcgaccg cgagagtcgc cttcggcacg acattgcgcg caatcggtcg cggttttggc
     2821 agcggaattt tcggtttctc cggcgctggc gcagccgagg tcgaccagcc gagcgccacg
     2881 gttaggctcg tcgccaggat gatggacagc acagtcgaca agcccgtcga ccgcaggaga
     2941 ggtgctcggt gagcaggggg cacggcggtc ctttgcggcg aatcgcgcga tcggtcttgc
     3001 tgtagttcac tgaaactacg gacaaatacc agactttatg gagcgccggg gttttgcgcc
     3061 gtcaccacgg caaaatcggg gctggagcgc agcgagttcc cagttggagg gcgggtcttt
     3121 cgccgcggcc acagacccga tatgaatcgt cttttcaggc agatgcgacg ccaatgaggc
     3181 agatgcgacg ccaaaatcaa gcgttgggag gagttttatg gccgccaaga ccaaattccg
     3241 gggttcgttt accgccttgg tcacgccgtt caagaacggc tcgctcgacg aggcggcctt
     3301 ccgcgcgctg gtgaactggc aaatttccga gggcacccac ggcctggttc cggtgggcac
     3361 aacaggcgag agcccgacgc taagccatga cgagcacaag caggtggtcg aatggtgcat
     3421 cgacgaggcc aaggggcgcg tgccggtgat tgccggcgcc ggctccaatt cgaccaagga
     3481 ggcgatcgaa ctcgccgagc atgccgagaa ggcaggcgcc aacgccgtgc tggtggtgac
     3541 gccctattac aacaagccga ctcaagaggg tctgtatcag cacttcaagg cgatcaatga
     3601 tgcgatcggc attccgatca tcatctacaa cattccgccg cgttctgtga tcgatatgtc
//
LOCUS       DATLJT010001346         3670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2408445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001346 DATLJT010000000
VERSION     DATLJT010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2408445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="VM910_30880"
     CDS             <1..1687
                     /locus_tag="VM910_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HVI66948.1"
                     /translation="VSILALFGALVAAFGNNLPLTLRARVLAVQAWVAGAFYLFILTT
                     SNPFLRLPDPPIEGRDLNPVLQDIGLAVHPPMLYLGYVGFSISFSFAIAALLEGRIDA
                     AWARWVRPWTLMAWIFLTLGIAMGSYWAYYELGWGGWWFWDPVENASLMPWLAGTALL
                     HSALVMEKRDALKVWTILLSILTFSLSLLGTFLVRSGVLTSVHAFASDPTRGVFILLI
                     LCVFIGGSLTLFAGRASALKQGGLFAPISREGALVLNNLFLTTACATVFIGTLYPLAL
                     EVLTGDKISVGAPFFNLTFAPLFIPLMVAVPFGPMLAWKRGDLLGAMQRLLAAGIAAL
                     LAIAVAWAWARGGSALAPLAIGLAVFVIAGALSDLAERVGLFRLPLASSASRAMGLPR
                     SVWGTAFAHAGIGVALIGIVCETTWNSEYIGTMKPGDAISVAGYQMTLEGLIQQQGPN
                     YRELVAPFTVRLDGERLAVMTPSKRTFTTRGMSTTEAALLTRGASQLYISLGDPTADG
                     GITARIYHKPLVLLIWFGPVLMAFGGLLSLSDRRLRVGAPKPAKAVRGLQAAE"
     gene            1691..2173
                     /locus_tag="VM910_30885"
     CDS             1691..2173
                     /locus_tag="VM910_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HVI66949.1"
                     /translation="MKRVVLAACLVALMGLSPARAVLPDEVMADPAKEARARLLSREL
                     RCMVCQNQSIDDSEAPLARDLRLLVRERIAAGDSDSQVIDFLVARYGEFVLLRPRFER
                     QTLLLWLVPPLLLVGGGLALWVYTRRRVKDGEAGKALTAQEQARLAALLSAETPSKEP
                     "
     gene            2514..>3670
                     /locus_tag="VM910_30890"
     CDS             2514..>3670
                     /locus_tag="VM910_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVI66950.1"
                     /translation="MTDRPTDLSSFPSYRQRGSLLSARKFALMASVVAGLGAAVYGFG
                     PSSGPASIFSSPAQAQVGNEVRKVAQPIGFADIVERVKPSVMSVKVNINEKITKDDSG
                     DDSPFQPGSPMERFFRRFGGPDGLPPGMRPRGHGVVTGQGSGFFISADGYAVTNNHVV
                     DGADKVEVTTDDGKTYSAKVIGSDARTDLALIKVEGGSNFQFAKLSDGKPRIGDWVLA
                     VGNPFGLGGTVTAGIVSAMGRDIGNGPYDDFIQIDAPVNKGNSGGPAFNTDGEVMGVN
                     TAIYSPSGGSVGIAFSIPAATVKSIVAQLKDKGSVSRGWIGVQIQPVTSEIADSLGLK
                     KAEGALVAEPQANGPAAKAGIESGDVITAVNGESVKDARELARTIGGLAPGT"
BASE COUNT          573 a         1163 c         1214 g          720 t
ORIGIN      
        1 ggtctcgatc ctggcgttgt tcggcgcgct cgtcgctgcc ttcggcaaca atctgccgct
       61 gacgttgcgc gcgcgcgtgc tggcggtgca ggcctgggtc gccggcgcct tctatctctt
      121 cattctcacg acctcgaatc cgttcctgcg tcttcccgat ccgccgatcg aggggcgcga
      181 ccttaacccc gtgctgcagg atatcggatt ggccgtgcat ccgccgatgc tctatctcgg
      241 ctatgtcggc ttctcgatct ccttctcctt tgcaatcgcg gcgttgctcg aaggccgcat
      301 cgatgcggcc tgggcgcgct gggtgcgtcc gtggacgctg atggcctgga tcttcctgac
      361 gcttggaatc gcgatgggtt cgtactgggc ctattacgag ctcggctggg gcggctggtg
      421 gttctgggac ccggtcgaga acgcgtccct gatgccgtgg cttgccggca ctgcgctgct
      481 gcattcggcg ctggtgatgg aaaagcgcga cgcactgaaa gtctggacca tcctgctttc
      541 gatcctgact ttctcgctgt cgctgctcgg cacgttcctc gtgcgctccg gcgttctgac
      601 ctcggtgcat gcgtttgcca gcgatccgac gcggggcgtg ttcatcctcc tgatcctctg
      661 cgttttcatc ggcggcagcc tgaccttgtt tgccggccgc gcctcggcgc tgaagcaggg
      721 cggcctgttc gcaccgattt cgcgcgaggg cgcgctggtt ctcaacaatc tcttcctgac
      781 gacagcctgt gcgaccgtct tcatcggcac gctctatccg ttggcgctgg aggtcctgac
      841 aggcgacaag atttcggtgg gcgcgccgtt cttcaacctg accttcgccc cgctgttcat
      901 cccattgatg gtcgcggtgc cgttcgggcc gatgctggct tggaagcgcg gcgatctcct
      961 gggagcgatg cagcggctgc tcgcggccgg catcgcagct ttgcttgcga tcgcggtggc
     1021 gtgggcctgg gcgcgcggcg gcagcgcgct ggcgccgctt gcgatcggac tggccgtgtt
     1081 cgtcattgcc ggcgcgctca gcgatcttgc cgagcgtgtc ggccttttcc gcttgccgct
     1141 tgcgagctcg gcgagccgcg caatgggctt gccgcgctcg gtctggggaa cggccttcgc
     1201 ccacgccggt atcggcgtgg cgctgatcgg tatcgtctgc gagaccactt ggaacagcga
     1261 atacatcgga acgatgaagc ccggcgacgc catcagcgtc gcgggatatc aaatgacgct
     1321 ggagggcctg atccaacagc aggggccgaa ctatcgcgaa ttggtcgcgc cgttcaccgt
     1381 gaggctcgac ggcgagcggc tcgcggtcat gaccccgtcg aagcggactt tcaccacgcg
     1441 cggcatgtcg acgaccgaag cggcgttgct gacgcggggc gcaagccagc tctacatctc
     1501 gctcggcgat cccacggccg atggcggcat caccgcacgt atctaccaca agccgctggt
     1561 gctcctgatc tggttcgggc cggtgctgat ggcgttcggc gggctgctat cgctgtcgga
     1621 tcggcggctg cgcgtcggcg cgccgaaacc ggcgaaggcg gtgcgcggtc tgcaggcggc
     1681 ggagtgaaac atgaagcgcg tcgtcctggc cgcgtgcctt gttgctctga tggggttgtc
     1741 gccggcgcgc gcggtgctgc cggacgaggt catggccgat cccgccaagg aagcgcgcgc
     1801 cagattgctg tcgcgtgaac tgcgttgcat ggtctgccag aaccagtcga tcgacgattc
     1861 cgaagcgccg ctcgcgcgtg acttgcggct gttggtgcgt gagcgtatcg ccgccggcga
     1921 cagtgattcc caggtgatcg atttcctggt cgcgcgctac ggggagttcg tcctgttgag
     1981 gccgcgcttc gagcggcaga ccttgctgct ctggctcgtg ccgccgctgc tgcttgtggg
     2041 cggcgggctc gcgctatggg tttacacaag acggcgcgtc aaagacggag aagcaggcaa
     2101 ggcgctgacc gcgcaggagc aagcgcggct ggcggcgctg ctatcggccg aaacgccgtc
     2161 gaaagagcct tgaaaggttc cgttcgtaac gggcggccgg caagaaaccg cgccgcgcaa
     2221 tggcgccggc acacttcgct gctgaccttg aaacacaagc tcgaaggctt catgttaagc
     2281 ttcaaggtga gctgagcgac gccccgattg tgggctatgg aacctgcagc aaatcaaaga
     2341 cttcggccct gttggtcggg cctgcgataa actgccgcat tcgatccctg cttcattaca
     2401 aaagtttaac tccgccgcca gcgcgccgta aggctgcaaa ccccatgttg atcggtgaaa
     2461 atgggaaaat cccaaaaaac tgattcgatt ccaatgccct ggagacacgc tgaatgaccg
     2521 accgtccgac cgatctttcg tcctttccct cctaccggca gcgcggttcg ctgttgtccg
     2581 cgcgcaaatt cgccctcatg gcctccgtcg tggcggggct tggtgcggcc gtctacggct
     2641 ttggcccctc gtcggggccg gcgagcatct tctcgagccc cgcgcaggcg caggtgggta
     2701 atgaggtacg caaggtcgct cagcccatcg gttttgccga catcgtcgag cgcgtcaaac
     2761 cctccgtgat gtcggtgaag gtcaacatca acgagaagat cacgaaggac gatagcggtg
     2821 acgactcgcc gttccagccc ggctcgccga tggagcgctt cttccgccgc ttcggtggac
     2881 cggacggcct gccgccgggt atgcgtccgc gcgggcatgg cgtggtgacc ggtcaggggt
     2941 ccggcttctt catttcggcc gatggctacg ctgtgaccaa caaccacgtg gtcgacggtg
     3001 ccgacaaggt cgaggtcacc accgacgatg gcaagaccta cagcgcaaag gtgatcggca
     3061 gcgatgcccg caccgacctt gccttgatca aggtggaagg tggctcgaac ttccagtttg
     3121 caaaactctc ggacggcaag ccacggatcg gcgactgggt gctggcggtc ggcaacccgt
     3181 tcggcctagg cggcacggtg acggccggca tcgtctcggc gatgggccgc gacatcggca
     3241 acggtccgta tgacgacttc atccagatcg acgcccccgt caacaagggc aattcgggtg
     3301 gtccggcctt caacaccgac ggtgaggtga tgggtgtcaa caccgcgatc tattcgccct
     3361 ccggcggcag cgtcggcatt gcgttctcga tccccgcggc gacagtgaag agcatcgtcg
     3421 cccagctcaa ggacaagggc tcggtcagcc gcggctggat cggcgtgcag attcagccgg
     3481 tcacgtccga gatcgccgac agcctcggct tgaagaaggc cgaaggtgca cttgtcgccg
     3541 agccccaggc caacggtccg gcggccaagg ccggcatcga gtccggcgac gtgatcaccg
     3601 cagtcaacgg cgagagcgtc aaggatgcgc gcgaattggc gcgcaccatc ggcggccttg
     3661 cgccgggcac
//
LOCUS       DATLJT010001347         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2868906,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001347 DATLJT010000000
VERSION     DATLJT010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2868906"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VM910_30895"
     CDS             complement(<1..309)
                     /locus_tag="VM910_30895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66951.1"
                     /translation="MKRRAFITLLGGAAAAWPLAARAQQPAMPMVGFVTGLSSNYVTG
                     RKPAFRRGLREVGYVEGQNVLVEYHSVEGQSDRLSGVVTDLIERKVAVIVAVGGTDPA
                     K"
     gene            668..1351
                     /locus_tag="VM910_30900"
     CDS             668..1351
                     /locus_tag="VM910_30900"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVI66952.1"
                     /translation="MTAVTPEPILKVAMGFMAAKHLFVASEVGLFEALAAGPATIEEL
                     ASQTGVPSRTIAIATAAMVSLGFIEQHGHHYQNGEQVAAFLAGKPGPDLRPMLRFFDQ
                     ISYPAWQKLADAVRTGNGQAQFGKFDKRQQKIFSAGVESFSGPGAAALAANYDFGRHH
                     KVLDVAGGTGSFLVAVLKRHAKLRGTLFELPGPCAVARQKLANLPEGSRIDIVEGDVF
                     KDALPAGRL"
     gene            1314..1658
                     /locus_tag="VM910_30905"
     CDS             1314..1658
                     /locus_tag="VM910_30905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66953.1"
                     /translation="MFSRTRCLPAGYDALIVANTAHVFSVPHNIELLRKMRTGVQPGA
                     RLLLVDLWTDLSHTQPTSAALISGEFLVISGEGQAYSEQEADTWLRQTGWKKIEKTPL
                     AGPTSLIVAEAV"
     gene            complement(1681..2025)
                     /locus_tag="VM910_30910"
     CDS             complement(1681..2025)
                     /locus_tag="VM910_30910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66954.1"
                     /translation="MAFGCSTSLARRSRISAHQIEFFVGQSFRSVLDCKFFRHSRGDD
                     LQIRRRLFQAHNFYHPGAVLGEVLAQRLEEIAAELVTRALFPAGSQVTKVKYPDFTLA
                     DDLVSQRAAGTQ"
     gene            2049..2540
                     /locus_tag="VM910_30915"
     CDS             2049..2540
                     /locus_tag="VM910_30915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66955.1"
                     /translation="MTEKPLTEAEQLDAALEKLIAERTRRRLATGKWVMPVVVIDNGE
                     QVSASSAEESPPQWKNPPELMEIHVIHPRPVVELPTEQWEQPVDARDVTPPRPPTSPR
                     SARSLPPAPGAGALSVDPRNVNIPPSIYSAEQRRTRNFNDDTWGDPAGWPIRYPRGNR
                     SGW"
BASE COUNT          559 a          826 c          837 g          521 t
ORIGIN      
        1 tttcgccgga tcggtgccgc ctactgccac gatcaccgca accttgcgct caatcaaatc
       61 ggtcacgacc ccggacagcc gatcggattg cccctcgacc gagtgatatt cgaccaagac
      121 gttttggcct tcgacatagc ctacctcccg caagccccgg cgaaacgccg gtttgcggcc
      181 cgtgacgtag ttggatgaga ggccggtgac gaacccgacc atgggcatcg ccggctgctg
      241 cgcgcgtgcc gcgagcggcc aagccgcggc cgcgccgccg agaagggtga tgaacgcgcg
      301 tcgtttcact gtccgatgcc tcccgtgctt ccgaacgaaa ggatagcaca ctcagctacg
      361 gcagaagact actgcgctgc gggatttcgg tccggccgat gtcagcttcg gtcaaagtcg
      421 gtcattcgac ggtgtctgcc ccatgtccgc ctttgccccc gaaagcggaa gtccgcccgc
      481 gatcttgcta tgtcacaaga gtgcagggag ccgacatcag tgatggtatg ttcattacgg
      541 tgccgtagtc ctcattcgtt ttcgacttgg cccttccgga tcgccttagt tttagcgggt
      601 ggagttgagg gccgactaca caagccgcaa tgtcgaaata cctagccgac cattggggcg
      661 atcaaccatg accgctgtca ctcccgagcc catcctaaag gtcgctatgg gcttcatggc
      721 tgccaagcac ctgtttgtgg ccagcgaagt cggtttgttc gaagcgcttg cggccgggcc
      781 ggccacgatc gaggagcttg cgagccagac cggtgttccg tcgcggacga tcgccatcgc
      841 gaccgccgcg atggtcagtc tcgggttcat cgaacaacac ggacatcact atcaaaacgg
      901 cgagcaggtg gcggcctttc tggccggcaa gccaggtccc gatctgcgac caatgctacg
      961 ctttttcgat cagatcagtt atccggcatg gcaaaagctt gcggacgcgg tgcgaaccgg
     1021 caatggccaa gcgcaattcg gcaaattcga taagcgccaa cagaagattt tctcagctgg
     1081 cgtggaatcc ttttccggcc cgggcgcggc agcgttggca gcaaattacg acttcggccg
     1141 ccaccataag gttctcgatg tcgctggcgg caccggctcg tttctcgttg cggtcttgaa
     1201 gcgccacgcc aaattgaggg gaacgctgtt cgaattgccg ggtccctgtg ccgtcgcgcg
     1261 gcagaagctc gcgaacttgc ccgagggttc gaggatcgac attgtcgagg gcgatgtttt
     1321 caaggacgcg ctgcctgccg gccggctatg atgcgctaat cgtcgcaaac accgcccatg
     1381 tcttctcggt accgcataac atcgaactgt tgcgcaagat gcggacaggt gtgcagcccg
     1441 gtgcacggct gttgctcgtc gatctgtgga ccgatctgag tcacacgcag ccgacttcgg
     1501 cagctttgat atctggagag ttcctcgtca tctctggtga gggccaagcc tatagcgagc
     1561 aggaagccga cacatggctc agacagaccg gatggaagaa gattgaaaaa acgccgctgg
     1621 cgggcccgac cagtctgatc gtcgccgaag cggtctaacg aaagtcacat cgtaacgtct
     1681 ttactgcgtg cccgccgcac gttggctgac gaggtcatcg gccaatgtga agtcggggta
     1741 tttgacttta gtgacctgtg agccggcagg aaaaagggct cgcgtgacaa gctcggccgc
     1801 gatttcttcc aggcgttgag cgaggacttc gccgagcacg gccccggggt gataaaaatt
     1861 gtgcgcctgg aagagccggc gacgtatttg aagatcgtcg cctcgactat gccgaaagaa
     1921 tttacagtcg agcacgctgc ggaagctctg accaacgaag aactcgattt gatgagccga
     1981 tatgcgcgaa cgccgcgcca aacttgtcga acaaccgaaa gccattcccc tgatcgaggg
     2041 caagaagaat gaccgagaaa ccgctcactg aagctgaaca gctcgatgcc gcgcttgaaa
     2101 agctcatagc tgagcgaaca cggcgccgtc tggcgacagg caaatgggtg atgcccgtgg
     2161 tagtcatcga taatggggag caggtatctg cgtcgtccgc cgaggaatcg ccgccgcagt
     2221 ggaaaaatcc gcccgagctg atggagatcc atgtcatcca tccgcgcccg gttgtcgagc
     2281 ttccgaccga gcaatgggag cagccggtgg acgcgcgcga cgtgacgccg ccgcgtccgc
     2341 cgacatcacc acgctcggcg cggtcgctgc caccagcgcc gggtgccggg gcgctttccg
     2401 ttgatccgcg caacgtgaac ataccgccct cgatctattc ggccgagcaa cggcggacga
     2461 gaaatttcaa tgacgatacg tggggcgatc cggctggatg gcccatcaga tatccgcgtg
     2521 gcaatcgcag cggatggtga gggccgcccg gtggcgtgcc gagcgacaga aagcgtttga
     2581 cctaggttca gcggcgggcg gcccgaccct gtggggctgg gcaatccttc ataaagccgt
     2641 aaccccgatt gagatcgaca tgctctacgc acttattgcc acggcgcatc ggaccgggac
     2701 cggcctgcca ctcccacggt gcctgcgctc cgtacggccc gga
//
LOCUS       DATLJT010001348         1966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1261383,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001348 DATLJT010000000
VERSION     DATLJT010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261383"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VM910_30920"
     CDS             <1..268
                     /locus_tag="VM910_30920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66956.1"
                     /translation="SSGADNVATFQKTSLSQRQYCKKCGGHLMTNHPPLGLIDVFAAT
                     LPDLAFKPGVHVNYAETVLPMRDGLPKLKDFPKEFGGSGEMLSE"
     gene            1152..1484
                     /locus_tag="VM910_30925"
                     /pseudo
     CDS             1152..1484
                     /locus_tag="VM910_30925"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT          380 a          643 c          609 g          334 t
ORIGIN      
        1 ctcatcgggc gccgacaacg tcgcgacgtt ccagaaaacg agcctgagcc agcggcaata
       61 ttgcaaaaag tgcggcgggc acctgatgac caatcatccg ccgctcggcc tgatcgatgt
      121 tttcgccgca acgctgcccg acctagcctt caagccaggc gtgcacgtca actatgccga
      181 gacagttctg ccgatgcgcg acggcttgcc gaagctgaag gactttccga aagagttcgg
      241 cggatcgggc gagatgctca gcgagtgatc aaagagcgat accggctcac gccggtgtca
      301 cctctcgaaa atccgcaggc ttcatatagc cgcacgccgg tctttccgca gggaccaccg
      361 acagcgcgtc aactgtcggc gcgatgggcg cggcagtcct gtccgcaaac ggcacgctca
      421 gaccacgcga aatcccgatc cgcaaaagcc tcgcctatca tcgccgcaat cggaccaacg
      481 cgacttccct aacgactttg tgaaacggct cgcagcgaac aacaaaatcc tgtgtttcct
      541 tgaacctgga caccttgtcg gcgaccaacg caggggaatt gcgtcgcctg accggggttt
      601 gcagagcgac gacggcgtcg atcggcgatc gtgcgcaatg ttctggagac gtgatgacga
      661 gcggggaagc ctatgtcgaa gccgtcgaag cgatctatgc gtgcggcacc ggggagggca
      721 acctgtccga cgcgctgatg gcgaccagcc gactgttggg cgcgcgtggg gcaacgcttg
      781 aagtcatcga caaatccggc ggcggcctga tctggttttg cgcagccgga cttcccgcac
      841 tcggccgctc aaaatattcg cggcaatttg ccgcgctcaa tccgcgcatt ccgccgatac
      901 tccgtcaacg cgccggcgac gtgagctggg actacaagct catggacgag gagacgatga
      961 ggcgggacgc cttctattcc gaattcctgc ccagcatggg gctgcgttat ttcatctcgg
     1021 cggtgctgga tcagacgccc gaccggatcg cggtcgtttc ggtgcagaga tcacgtcgaa
     1081 aaggtcatgt cacgcgtcgc gagatcgcgt tgatgcaccg cctttgcccg cacctccaaa
     1141 gagcctacag gatgagaacg ctgcttgcac tcgatgcccc tccgcgcgaa gccgtgtttg
     1201 aagatgcgct cgatctgatc gccgacggca tcgcgctgtt gcggcgggat ggccggatca
     1261 tctatgccaa cgaggtgctg cggcggcttg ccgagcgcag caccggcttc cggatcggcg
     1321 gcgagacgat cgaattcctg aacccggacc tgcgcgaccg cctggccagc gccttgagcg
     1381 cggtcgatcg cgtccgcgat ccggccgcag ccttcctcgc caccgatttt gcggttccgc
     1441 gcgaggacgg cttgccgccc tacacggtgt cgctgcggcc gttaccgcgc gccggagacc
     1501 gagcgagcca tccggacgcc gcggcgatgc tgttggtgca cgatccgctc gagcggagtc
     1561 tcgccgccgg gaaaatgctg caggaactct atggcctgac caatgccgag gcgcaacttg
     1621 tgcaggcgct cagcaccggc atgacggcgg tcgcctacgc gcagagccgc ggcgtcagca
     1681 tcaccaccgt ttacactcat ctgcggcgga cccgcgagaa gacgggatgg aagagcgtcg
     1741 ccgaactgac gcgccgcttc cacgagctga acgtcgcgct gcgcgccact gaagcggccg
     1801 caattcaccc ttcgccacgc aagcaagacg cgaacgcgtc gatgcgcgcc tgcaacggct
     1861 ctcgcacccg atcgagcacg cttgcgtaat cgcggcgttc atcctgccgg aacagccgca
     1921 cgctcggata ccaggggctg tcgtcgcggt tgagcaacca gcgata
//
LOCUS       DATLJT010001349         7746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3327813,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001349 DATLJT010000000
VERSION     DATLJT010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3327813"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VM910_30930"
     CDS             <1..1029
                     /locus_tag="VM910_30930"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HVI66957.1"
                     /translation="AEEVPVQLAVKSKAERGYTLIAGEAPQAVKAAPVALTPDCSRQA
                     AFQAIARACLRQLVANQPATLGGDPEGLHQMRVSLRRLRAAVSLFADMLVDPQTEEMK
                     TQFKWITQELGPARELDVFIKRVVRPVTDGKPNGPGVAVLAKDLQRRREEAFARACAA
                     VESTRFRGLVLDVAGWIEAGDWTRSTDDFARLLREQPIVPAAADELRRRWKKILKAGA
                     QLDELDPQRRHKMRIQAKKLRYASEFFGGAFPGKKAMRRRKGFVAGLAKLQDALGDLN
                     DIAVHEGLSERIVDAQDASGKQREGRAKKAFAAGRLSGREEARIASVLKDAQLAYRAF
                     TKAKPFWS"
     gene            complement(1657..1998)
                     /locus_tag="VM910_30935"
     CDS             complement(1657..1998)
                     /locus_tag="VM910_30935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66958.1"
                     /translation="MVAEAGGERYYFGDRINGYLVQQSGEYAYPLWGFIAAAALQAGM
                     PEKDMPDVREMFAHVAKTIGTLATHEGAVEQRRRRPEGFERPACQRPDRPRIGARGTD
                     KRPRGALAWRG"
     gene            2016..2411
                     /locus_tag="VM910_30940"
     CDS             2016..2411
                     /locus_tag="VM910_30940"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HVI66959.1"
                     /translation="MASQEDRSSETLHSAPQSRAEDPRARHMRAGVEKLSKEMGEPRW
                     YEILQAVVRAMAPYARHGVNVNVDFYSGVVYHLLGIKRDLFVPIFAVGRVPGWVMQVL
                     EQMENNILIRPLTLYCGPQPRSYRPISAR"
     gene            2558..3514
                     /locus_tag="VM910_30945"
     CDS             2558..3514
                     /locus_tag="VM910_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI66960.1"
                     /translation="MHVVRKLALLSVVACAGSALAAVPVRAAYPERPIELIVPWGPGG
                     GADQLARLVSKLAEPLLSQGIPVVNVPGATGGTGMAKLLAAPADGYSAAIYIADSHVL
                     LAGKNPRWSMSDIVPVAVMIEGPSFIFVAQDSRFKTWQDFENEAKANPGKPKIATLGF
                     GSVDDLSISVIERSKGLKFVQVPFSKPSERYVSILGGHADALYEQAGDVASFLNNKQM
                     RPLLIFGKQRLPAFKDTPSSYELGIPIALPQFRAIVVRAGTPADIIERLSKTLADIAH
                     TDEYKKFLEEQFAVPTSFKDASMAPAYVSQQLEEMKKAMAAQ"
     gene            3537..4079
                     /locus_tag="VM910_30950"
     CDS             3537..4079
                     /locus_tag="VM910_30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI66961.1"
                     /translation="MDKALFRSIAPYVVGLAVAGALYIYSGTFEYTPRPGALGPEVWP
                     RLAILLIAASCLFELTRRLIIGGKEATGFMEAFDREPQTEQAQPVYPRLVIGGVVLMA
                     FYAVLVPILGFILATFLFLAAFMYVGGYRAHGAVWGASAAVTVFCGILFLRIAYVSLP
                     RGIEPFDRLTDAFFAVPSLW"
     gene            4084..5586
                     /locus_tag="VM910_30955"
     CDS             4084..5586
                     /locus_tag="VM910_30955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI66962.1"
                     /translation="MIDIVSQLAAGFVHCLTPLNMAMLLIGIVIGLLIGVLPGLTLVM
                     GVTLALPFTYKMDVTASIVLLTAMYVSGTYGGAFTAILFRIPGEPIDVPMLWDGYTMG
                     RKGRPAKALGWTLVAALGGGLLSAVVMVALTQPLARFALRFSSPEYFVIVMFGLLSVV
                     AIGRGSLANAFISMSVGILISSVGVDPVYGTYRFTFGSPILADGIEFLVVMVGAYGVG
                     EVLTRLETGFAAKPLDKISNARTELPTLKELKEIKGMFLRSSVIGDLIGLLPGAGATI
                     ASFVSYGIEARFGKRRDQMGTGVAEGIVAPQSAATASVGGAMVHLLALGIPGSGATAV
                     ILGAFMLHGIQPGPQVLVKSADMVYTVFASLFLGLALMCAIGYFAIRPLVKILDFPEA
                     VVSAYVLILCFIGALSIRNNVTDLWLIIGFGILGYGFERLKFPISPLVLGAILGPIAE
                     EAFMNTMISFSNDWTVFFTRPISGTIVALTVIVLLLPFVQRLMRGRLART"
     gene            5895..6191
                     /locus_tag="VM910_30960"
     CDS             5895..6191
                     /locus_tag="VM910_30960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66963.1"
                     /translation="MLSTAWVQRPIQKRDAKKWPWSGILHMAGFFPSVPTQVNFELAY
                     APVGGRWRLFALSVSFGQAAPAAPQPPPSAPQAAASAAPHPPAAPAKTNPAPKP"
     gene            6248..7027
                     /locus_tag="VM910_30965"
     CDS             6248..7027
                     /locus_tag="VM910_30965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66964.1"
                     /translation="MLPVSALKSMRVMFATPCYISAVSMNYVASIFSLSLDCAHVGLP
                     CILHLHSESLITRGRNKMVIKFLSEDFTHLFWIDSDIVFGSQSVFRLLLADRDVAAGV
                     YPMKNFKWPAEGLPAGMTQKQFENTYGEYPFNPIDHGASRVGRYVDSEGFIEVAEAPT
                     GFMVIKRQVFPMMMKHYPELNYVPDGPPNHPQAHLHWRFYDCMVDPESGRYLSEDYTF
                     CRRWRDMGGKIWVDLDCKLMHLGQHLFRGDLAESLRVQGRW"
     gene            7074..7157
                     /locus_tag="VM910_30970"
     CDS             7074..7157
                     /locus_tag="VM910_30970"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66965.1"
                     /translation="MINLKTAKSLGLEIPATVLARADEVLE"
     gene            7235..7411
                     /locus_tag="VM910_30975"
     CDS             7235..7411
                     /locus_tag="VM910_30975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66966.1"
                     /translation="MGEVTTIGLDLAKHVFQVHGVDSRSLFDAVAVMTGLTPLVSELE
                     ARLAADVEFKGVDA"
BASE COUNT         1396 a         2362 c         2425 g         1563 t
ORIGIN      
        1 gccgaggaag tgccagttca gcttgcggtc aagagcaagg ccgagcgcgg gtacacgctc
       61 atcgccggcg aggcgcccca ggcggtaaag gcggcaccgg tcgcgctcac gcccgactgc
      121 agccggcaag ccgcatttca agccattgcc agggcgtgtc tgcgccaact cgtcgccaat
      181 caacccgcca cgctcggcgg agaccccgag ggattgcacc aaatgcgggt ttctcttcgg
      241 cgtttgcggg cggcggtctc gctgtttgcc gacatgcttg ttgatccgca gacggaggag
      301 atgaagaccc agttcaagtg gatcactcag gaactaggcc cggcgcgcga acttgacgtg
      361 ttcatcaagc gcgttgtgag gccggtcacg gatggtaagc cgaatggacc gggagtggcg
      421 gttcttgcca aggacctcca gcgaagacgt gaggaggcgt ttgcccgcgc ctgcgcggcc
      481 gtcgaatcca cacgctttcg tggtcttgtt ctggacgttg cgggctggat cgaggccggc
      541 gactggacgc gcagcactga cgatttcgcc cgcttgctgc gagagcagcc gattgtgcct
      601 gctgccgccg acgagttgag gcggcgctgg aagaagattt tgaaagctgg cgcgcagctc
      661 gacgagctcg acccacagcg tcgacataag atgcgcattc aagccaagaa actgcggtac
      721 gcctcggagt tctttggcgg cgcgttcccc ggcaagaagg ctatgcggcg acggaagggt
      781 ttcgtggccg gattggcgaa attgcaggac gccctcggtg acctcaacga catcgcggtg
      841 catgagggac tgagcgaacg gatcgttgat gcgcaggacg ccagcggcaa gcagcgcgag
      901 ggccgtgcca agaaggcatt cgctgccggt cgtctttctg gccgtgagga ggcgcgcatc
      961 gcatccgtgc tcaaggatgc gcagcttgcc taccgggcct tcactaaggc gaagccattt
     1021 tggtcgtgat ttcgaggcca agcttgctgc cgccgcgtcg agacggtgac gaccctcgtt
     1081 cgcggagagg atatgattga ggcgggagcg acaccgcctt gttcatccac agcgcgccga
     1141 cgaggggtta tctggcgccg gtttattttt gagggcgaca gcggtcccgt agagaccgat
     1201 attatcttag aacctgatcg agataggccg aagctgccgc gaaggaggcg attgagcgcc
     1261 tcagacgaat gccgccaaga gcgcatttcc ctaaaatggt ccccggagca gttggccaca
     1321 acgaccacga ggaccacgcg gcctcctaag gggttccttt gctaggcaaa aatcgcgata
     1381 cgtccggcaa tcgctgtctg attgcctctc tgtggcactg gttggcgcag cagttcatca
     1441 ggatggcgaa ggacaccctc gatccgcgcc tggcgttttc cttggtcatg gaacccgcga
     1501 tcatgatgtc gaaggtcgat ccgaagagca ttccacagac gcgcccgtcg gatggctgag
     1561 atcaggcgcg ccgagcaata cgaccgtcta cgtgaggcca tggaggccaa tgccgctatc
     1621 accgtcgttc gagccgaagc cgtacggcac gcgatattat ccacgccaag ccaatgcgcc
     1681 tcggggacgc ttgtccgtcc ctcgggcgcc gattcgaggc ctgtcaggcc tttgacaagc
     1741 agggcgctcg aagccttctg gtcgccgtcg gcgttgctca acagcacctt cgtgcgtggc
     1801 gagtgtcccg attgttttgg cgacatgggc aaacatctcg cggacatcgg gcatgtcctt
     1861 ttccggcatc ccggcctgaa gcgccgctgc ggcgataaat ccccacagcg gataggcgta
     1921 ttcaccagac tgctgcacca ggtaaccgtt gatgcgatcg ccgaagtagt aacgttcgcc
     1981 gcccgcctcg gccaccacga ttgcgccgtt tggtattggc gtcccaggag gaccgatcga
     2041 gcgaaacgct gcattctgcg ccgcaaagcc gcgcagaaga tccgcgcgcc cgacacatgc
     2101 gcgccggggt ggagaagctg tcgaaggaga tgggcgagcc gcgctggtac gaaatcctgc
     2161 aggcggtcgt gcgggcaatg gcgccgtacg cgcgccacgg cgtcaacgtg aatgtcgatt
     2221 tctattccgg cgtcgtctac cacctgctcg gcatcaaacg ggatctgttc gtgccgatct
     2281 tcgccgttgg ccgcgtgccg ggctgggtga tgcaggtgtt ggagcagatg gagaacaaca
     2341 ttctgatccg gccgctcacg ctctattgtg gcccgcagcc gcgcagctac cggccgatca
     2401 gcgcgcgcta gcttggtgcg gtatctcgct ggctgagaag ctgcgtcgca ttgcgcgttg
     2461 cgggcttgtc ccgttctgct taaagctttg caaaagacag tctcgtggaa caacaagtgg
     2521 ccaccgccac agaattcggc ggtccaggga ggataacatg cacgttgtgc gcaaacttgc
     2581 tctgctgagt gtcgtcgctt gcgccgggtc ggcgcttgca gcggtcccgg tacgcgcagc
     2641 atatcctgaa cgccctatcg aattgattgt cccctggggt cctggcggcg gtgcggatca
     2701 acttgcgcgc ctggtcagca agctggccga gccgctgctc agccagggca ttccggtcgt
     2761 gaacgtgccg ggcgcgaccg gcgggaccgg catggccaag ctcctggcgg ctccggccga
     2821 cgggtattcc gccgcgatct atattgccga cagccacgtg ctgctcgccg gcaagaaccc
     2881 acgctggagc atgagcgaca tcgtacccgt cgccgtcatg atcgaaggcc cctcattcat
     2941 ctttgtcgcc caggacagcc gcttcaagac gtggcaggac ttcgagaacg aagcgaaggc
     3001 caatcccggc aagccgaaga ttgcaacgct cggctttggc agcgtcgacg acttgtccat
     3061 cagcgtgatt gaacgcagca aagggttgaa gttcgtccag gtgccctttt ccaagccgag
     3121 tgaacgctat gtctcaattc tcggcgggca cgccgacgcg ctctacgagc aggcgggcga
     3181 tgtcgcctcc tttctcaaca acaagcaaat gcggccgctg ctcatattcg gcaagcagcg
     3241 cttgcctgct ttcaaggaca cgccatcctc gtacgagctg ggcattccga tcgccttgcc
     3301 gcagttccgc gcgattgttg tgcgcgccgg cacgccggcg gacattatcg agaggttgtc
     3361 caaaacgttg gccgatattg cgcacaccga cgaatacaag aagttcctgg aggagcaatt
     3421 cgccgttccg accagcttca aagatgcctc catggctccg gcgtacgtga gccagcaact
     3481 cgaggagatg aaaaaggcga tggcggccca ataaggtgcg gatcgagcaa gcaggcatgg
     3541 acaaggcgct gtttcgctca atcgctccct atgtggtcgg cctggccgtc gccggcgccc
     3601 tctacattta ctcaggaacg ttcgaataca cgccgcggcc gggcgcgctc ggccccgagg
     3661 tgtggccgcg ccttgccatt ctcctgatcg ctgcgtcctg cctgttcgag ctgacgcggc
     3721 gcctgatcat cggcggcaag gaggcgaccg gcttcatgga agcattcgac cgagagccac
     3781 aaacggagca agcacagccc gtctatcctc gcttggtcat aggcggtgtc gtgctgatgg
     3841 cattttatgc ggtgctcgtt ccaattctcg gcttcatcct ggcaaccttc ctcttcctcg
     3901 ccgccttcat gtacgtcggc ggttatcgcg cgcatggcgc cgtttggggc gccagcgccg
     3961 cagtgacggt cttctgcggc attctgttcc tgcgcattgc ctatgtgtcg ctgccgcgcg
     4021 gcatagagcc attcgatcgc ctcaccgacg cgttctttgc agtccccagc ctgtggtgac
     4081 cgcatgatcg acatcgtcag ccaactcgcg gccgggttcg tccactgcct gacgccgctg
     4141 aacatggcaa tgctgctcat cggcatcgtc atcggcctgc tgatcggcgt gctgcccggc
     4201 ttgacgctgg tgatgggcgt cacgctggcg ctgcctttca cctacaagat ggacgtcacc
     4261 gcctcgattg tcctgctgac ggcgatgtat gtctccggca cctatggtgg cgccttcacc
     4321 gccatcctct tccgcattcc gggcgagccg atcgacgtgc ccatgctgtg ggacggctac
     4381 accatgggcc gcaaaggcag gccggcgaag gcgctgggct ggacgctggt tgccgcgctg
     4441 ggcggcggct tgttgtcggc agtggttatg gtcgcattaa cacagccgct cgccaggttc
     4501 gcgctgcgct tttcctctcc ggaatatttc gtcatcgtca tgttcggctt gctcagcgtg
     4561 gtggccatcg gcagaggttc gctggccaac gccttcatca gcatgagcgt cggcatcctg
     4621 atctcgtcgg tgggcgttga tccggtctac ggcacctatc gtttcacctt cggctcgccg
     4681 atcctcgccg acggcatcga attcctcgtg gtcatggtcg gcgcctacgg cgtcggcgaa
     4741 gtgctcacgc ggctggagac cggatttgcc gccaagcccc tcgacaagat ctccaacgcg
     4801 cgcaccgagt tgcccactct gaaggagttg aaggagatca agggcatgtt cctgcgctct
     4861 tcggtgatcg gcgatctcat cgggctgctg cccggtgccg gcgccaccat cgcttcgttc
     4921 gtgagctacg gcatcgaggc gaggttcggc aagcgacggg atcagatggg caccggcgtg
     4981 gcggagggca tcgtcgcgcc gcagtcggcg gcaacggctt ccgtcggcgg ggccatggtt
     5041 cacctcctgg cgctcggcat tccgggcagc ggcgcgaccg cggtcatcct cggcgccttc
     5101 atgctgcacg gcattcagcc cggaccgcag gtgctggtga agtcggctga tatggtctac
     5161 accgtgttcg cctcgctttt cctcggtctc gcgctgatgt gcgcgatcgg ctatttcgcc
     5221 attcgcccgc tggtgaagat tctcgatttt cccgaggctg tcgtatccgc ctatgtgctt
     5281 atcctctgct tcatcggcgc cttgtcgatc cgcaacaacg tcaccgattt gtggctgatc
     5341 atcggcttcg gcatcctcgg ctacggcttc gagcggttga agtttccgat ctccccgctg
     5401 gtgcttggcg cgattctcgg accaatcgcc gaagaagcct tcatgaacac gatgatcagc
     5461 ttctcgaacg attggaccgt atttttcacg cggccgatct ccgggacgat cgtggctttg
     5521 accgtgattg tgctgttgct cccctttgta cagcgcctga tgcgggggcg tttggcgagg
     5581 acttgaaggt ggcggaaagc gccggtattg accccgtccg gggatgatgt cttttgaaat
     5641 tcccagtctt ataaaatgag aattttgccg cgctaagcct tcgctaagtc tgtagaacgc
     5701 ggcgcgtcca cgagcctgca ccagcctcga cgcgaggcgt cagcgatcat ctatgctagc
     5761 ggtccagaaa atcgatttgt gcgaaaatcc cgaattgatt ggccggccac cgtcaatgaa
     5821 aatgggtact attgcctcgc cgtggcgata tgatgcaggg gctggcgcta tgatgcaggg
     5881 ggcccgccag ggtcatgttg tcgacggctt gggtccagcg acccatccaa aaacgagacg
     5941 ccaaaaagtg gccctggagc ggcattctgc atatggcggg cttctttccg tcggtgccca
     6001 cacaggtgaa tttcgaactg gcctatgcgc cggtcggcgg gcgctggcgg ttgttcgcgc
     6061 tctcggtctc gttcggccag gcggcgccgg ccgcgccgca accgccgccc tccgccccgc
     6121 aggcggcggc atccgccgcg ccgcacccgc ccgccgcgcc ggcaaagacg aatcctgcgc
     6181 caaagccttg acatgcggta cgccgcaccg ctcaaccgct ctggcaccac gacgaggcga
     6241 ccatcccatg ctgcccgtgt cagctctgaa atcgatgcgc gtgatgttcg cgaccccctg
     6301 ctacatctcg gcggtcagca tgaactatgt cgcgagcata ttcagcctga gtctcgattg
     6361 cgcgcatgtg ggccttcctt gcatcctgca tctccacagc gagagcctga tcacgcgcgg
     6421 ccgaaacaag atggtgatca agttcctgtc cgaggacttt acccacctgt tctggatcga
     6481 ttcggacatc gtattcggct cgcagtcggt ctttcgcttg ttgcttgcag acagagacgt
     6541 cgccgccggc gtttatccga tgaagaattt caagtggccg gccgaaggtc tccccgccgg
     6601 catgacgcag aagcaattcg aaaacaccta tggcgaatac ccgttcaatc cgatcgatca
     6661 cggagcgagc cgcgtcggtc gatatgtcga tagcgagggc ttcatcgagg tcgccgaagc
     6721 gccgaccggt ttcatggtga tcaagcgcca ggtgttcccg atgatgatga agcactatcc
     6781 ggagctcaac tacgtgccgg acggcccccc gaaccatccg caggcgcacc tgcactggcg
     6841 gttctacgac tgcatggtcg atccggagtc gggcagatac ctgtccgagg actatacctt
     6901 ctgccgtcga tggcgcgaca tgggcgggaa gatctgggtc gatctcgact gcaagctcat
     6961 gcatctcggg cagcatctgt tccgcggcga tctcgccgaa agcctgcgcg tgcagggtcg
     7021 ttggtgagaa gccggccgac ctgccggtgc aggcggcgac caagtacgag ctggtgatca
     7081 acctaaagac cgctaagtcg ctcggcctcg aaatcccagc tactgtgctc gcccgcgccg
     7141 acgaggtgct cgagtgatgg ggtggatggc ccccttgcgg catctgagtg ccaaactgct
     7201 gtctgatcga atcagcagcg aatgggagcc atccatgggc gaggttacta cgatcggatt
     7261 ggacttggcg aagcatgttt ttcaggtgca cggggtcgat tcacggtcgc tgttcgatgc
     7321 ggtcgcagtc atgaccggcc tgacgccgct ggtaagcgag ctcgaggccc gactcgcggc
     7381 cgatgtagag ttcaagggcg tcgacgcgta gcatcacgca ggcgcggggc cggcgaccca
     7441 cgccgttttc ctcgaggcat catctttcag gacaggagcc agactatgct cggacccaac
     7501 aaagaaatgg cgagggtggc acgtaactta ataaagatgt tcgactaacc gtgcaggcgc
     7561 ttgaggcgcg cggggtgctg agacgcaaag cagaactaga gatcgggagt gcgtttctcg
     7621 gatgtctttg ggaggcacgg aatggaatgc ccgatcggtg gcccgcggtg cttcgagggt
     7681 tgcgcgaagg gaaatctagc gctgaattat tccccgatga gctatacgac ggaaaggccc
     7741 tcggtt
//
LOCUS       DATLJT010001350         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_799059,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001350 DATLJT010000000
VERSION     DATLJT010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799059"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VM910_30980"
     CDS             complement(<1..544)
                     /locus_tag="VM910_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVI66967.1"
                     /translation="MTKVLMRSVCVGAILAVTGISAASAQQTIAPASATPVPGPMPDI
                     LQKYAPVTTERLKKPENGNWLMFRRTYDGWGYSPLEEINRDNVSRLQPVWSFATGQTE
                     GHQAPPIVNNGVMFVATPGNQLLAIEAKTGNVLWRYKRPFPEDMTPLHPTNRGVGLYG
                     DKVYFAAAEAILVALDAKTGK"
     gene            complement(645..1244)
                     /locus_tag="VM910_30985"
     CDS             complement(645..1244)
                     /locus_tag="VM910_30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVI66968.1"
                     /translation="MGKAGQAADRACNGLRIEASTRRGFLTVVSVLGLDLAALPAWAQ
                     PASERPKEEDFLVSIESDTPDALGLKDIPIGGPPVLAWPMDPASNTVRKDSRLNKILL
                     VRLDPATLVGSTKDRAADGVVAYSAICPHAGCEVNGWVAEQQILECSCHYSHYNPREG
                     AAVMDGPASRALPALPLKLVDGKLAVAKPFTSRVGIVPS"
     gene            1567..>2280
                     /locus_tag="VM910_30990"
     CDS             1567..>2280
                     /locus_tag="VM910_30990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI66969.1"
                     /translation="MNEAFFAELSEWLTQAGLAGTSETDILSGFCEHCVAAGLPLARA
                     LMFIDTLHPVHEGRLFRWGYGPTEAPLLEYGRTSPDALAASGSAPQDVELAERWRNSP
                     HYKMLQTGDSFLRRRLNAKAEAEFPVLSDFRAAGMTDYVSIITRFAPQGVIGEMDGVY
                     SSWATGAPDGFSDDQVTALERIAPYLALALKSVSLARMTGTLMETYLGRDAGRRVLGG
                     RIVRGIAERIDAVVWFSDLR"
BASE COUNT          386 a          747 c          717 g          430 t
ORIGIN      
        1 ccttgccggt tttcgcatcg agcgcgacca ggatcgcctc cgccgccgcg aaatagacct
       61 tgtcaccgta aagaccgacc ccgcgattgg tcggatgcag cggcgtcatg tcttcgggga
      121 aggggcgctt atagcgccac aggacgttgc cggtcttggc ctcaatggca agcagctggt
      181 tcccgggcgt cgccacgaac atcacgccgt tgttgacgat cggaggcgcc tgatggcctt
      241 ccgtctgccc ggtggcaaaa ctccagactg gctgcaaccg gctcacgttg tcgcggttga
      301 tctcttcgag cgggctgtag ccccagccgt cataggtgcg gcggaacatc agccagttgc
      361 cgttctccgg ctttttcagt cgctcggtgg tgaccggcgc atacttctgc aggatgtccg
      421 gcatcggtcc cggcaccggc gtcgccgagg ctggtgcgat ggtctgctgc gcggacgctg
      481 cggatatgcc cgtcactgcg aggatagcgc ccacacacac gctgcgcatg agaaccttgg
      541 tcatgacgtt tcctccttgt cccaagcaag actccaccgg gttttgtctg aattgctgcg
      601 ttgcgactgt cccctccggg tttttcgagc gccgagcgac ggcatcaact cggaacgatg
      661 ccgacgcggc tggtgaacgg tttggccacc gcgagcttgc cgtcgacgag cttaagcgga
      721 agcgccggca aggcgcgcga ggcgggaccg tccatcaccg ccgcgccctc gcgcggattg
      781 tagtgcgaat aatgacacga gcactcgaga atctgctgct ctgccaccca cccattcacc
      841 tcgcaaccgg cgtgcggaca aatcgcggaa taggcaacga cgccgtccgc cgcgcgatcc
      901 ttggttgatc cgacgagcgt cgccggatcg agccgcacca ggaggatttt gttgaggcgc
      961 gaatccttgc gaacggtatt gctggcggga tccatcggcc acgccagcac cggcggcccg
     1021 ccgattggaa tgtctttgag ccccagcgcg tccggcgtgt cgctctcaat tgagacgagg
     1081 aaatcctcct ccttgggccg ctcgctcgcc ggctgcgccc aggcggggag ggccgcgaga
     1141 tcaagtccaa ggaccgatac caccgtcaga aagccgcgcc tggtcgacgc ctcgatacga
     1201 aggccattgc acgcgcgatc ggccgcttgc cctgccttgc ccatcgccca gctcccaccg
     1261 cggcagctcg aactgtcggt gggccctacc tcggcggcgc actgccgcga ttgagatttg
     1321 atcgccggag tcccgtgctc gtaccgcagt ctttcggcac cggtagtcaa gcctgacggt
     1381 catcaaactg caagtcgctc gccgacgaca accaggtcag ccatatggac cgattattcg
     1441 gactttgcgg attcgattcg atatccgagg cacgtctgct tggcgccgaa agcggacgca
     1501 aattgaaagg ccaaggggca tgtcgccaac tgccatcacc gggcgttgtg gctgtataat
     1561 cgcggtgtga acgaagcctt cttcgccgaa ttgtcggagt ggctgaccca ggctggtctg
     1621 gccggcacgt cggaaaccga catcctgtcc ggattctgcg agcattgcgt cgcggccgga
     1681 cttccgcttg cgcgcgcgct catgtttatc gacacgctgc atccggtcca cgagggccgc
     1741 ctctttcgct ggggctatgg tcccaccgag gcgccgctgc tcgaatatgg ccgcaccagc
     1801 ccggacgcgc tcgccgcatc gggctccgcg ccgcaggatg tcgagttggc cgagcgctgg
     1861 cggaacagcc cacactacaa gatgctgcaa accggcgact cgtttctgcg ccggcgcttg
     1921 aacgccaaag ccgaagccga gttcccggtg ttgtcggact tccgggcagc gggcatgacc
     1981 gactacgtct cgatcattac ccgctttgcc ccgcaaggtg tcatcggcga gatggatggc
     2041 gtgtactcgt catgggcaac gggggcgccc gacgggttca gcgacgacca ggtgaccgcg
     2101 cttgagcgca tcgcgccata tctcgcgctc gccctcaaat cggtgtcgct cgcgcgcatg
     2161 accggaacgc tgatggaaac gtatctcggt cgcgacgccg gccggcgcgt gctcggcgga
     2221 cgcatcgtgc gcggaattgc cgagcgcatc gacgccgtcg tttggttcag cgatttgcgc
//
LOCUS       DATLJT010001351         7474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3100425,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001351 DATLJT010000000
VERSION     DATLJT010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3100425"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VM910_30995"
     CDS             <1..895
                     /locus_tag="VM910_30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVI66970.1"
                     /translation="TVALVADSVDVVKEGDALKQVPDNKIYVIDMTANPPKLAATLTG
                     GKQPSGLSFSPDGKMALVANRGDNSVSVLSVNGTDVKITDTVSLPDSVAHVVFTPDGK
                     RALAVRFPAHKVSVLDIADGKVTYNKVDLATGQWPYNVVVTPDSKLALTADNGAAGSS
                     DGSVDTTSVIDLEANPPRIIDRVVIADGPEGLAMSPKGDLAVAAIQRGSNMKKAFFHE
                     KNGSLSVLKIDGKKVTKTQDIELGGLPEAVMFTPDGKYILAGNFMTDDFSILKVDGTK
                     VTDTGKRFKVPGHPASARMGH"
     gene            complement(1110..2006)
                     /locus_tag="VM910_31000"
     CDS             complement(1110..2006)
                     /locus_tag="VM910_31000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVI66971.1"
                     /translation="MRRIRPTRLCRSWLFLEGANEPVLQAAALSGADVLIQELEDFTS
                     PAQRPAARALAADLFAAWRAAGVVVAVRVNPLDRDGMEDLAAVMRGRPDIVALPKVAE
                     PAHIVRLDEAVTRFERDYGIAEGSTALLPNIELARGLLQTHAIAKASPRTVACLMASE
                     DLAADLGAERGPDGAELAYCRQRFIVECVAAGVVAVDCPYTFSDGEGVERDTRWAHRL
                     GYAAKSLVDPAHAGVVNGVLTPSEQELSRAREIVEAFEAARAEGRSRAELKGSLVEVP
                     TYANAKRLIARAEALQQWARGS"
     gene            complement(2046..2528)
                     /locus_tag="VM910_31005"
     CDS             complement(2046..2528)
                     /locus_tag="VM910_31005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66972.1"
                     /translation="MNHVMKFCLSIALSFVTLVSFARAEELFKLSHNERIACSRGLAP
                     GKLNTATCKSYAYVFNVKTSEYFRCQVSLALTRDNKEVINVQADGSCAKKPRVFETDS
                     NYTFDATETEPPNTNSFFGQGGYAMWVADTTAQKVRGCITISSGLGSDVSKCLDMTFQ
                     "
     gene            2724..3029
                     /locus_tag="VM910_31010"
     CDS             2724..3029
                     /locus_tag="VM910_31010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66973.1"
                     /translation="MTRNSIPTLLLVAALGGLTTSAFAQAAGPTPWELKPDTGYAYDK
                     EGKTFVYKMGTSNAGLLLKGAKKVPKGTLFFIGQNGQLYMRSGPYLEGDGRFMFGSN"
     gene            complement(3130..4329)
                     /locus_tag="VM910_31015"
     CDS             complement(3130..4329)
                     /locus_tag="VM910_31015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66974.1"
                     /translation="MLTKAIRRHLTILALLLPFASTRAGDVLPMAVAEAEASPQAATW
                     KEYLAAREAFEAERDAYWTSIADKRRARNTKRRNQQQITLDDYVLTQPPVYTGPARPP
                     GPSPEPLPEHARPQPHRPIPVVADLVQAASEYFQWAPQRPASEIDFKRAYARYASAAG
                     LTREQAVRVYAFETGGDGTHASQSGFHGSHAISSAIGYNQLLTTNTVELLAEQGDEFV
                     KELGERAAGLTGPARKALEHKIAVLKKMVALATSVPDDWSLHEKIGDTPQGWGMHAMV
                     LDIDVGPMLQTHKLLTSVIFARQKGYMRPLTAAELEMMNLTGDGTGLDMVTMPLAMRE
                     QVPTSNFFQRRGYERNPVAINHNTVAKLLAVTDSRMDFNSNKVGAKELAMVFESGAGQ
                     ASARGDQ"
     gene            complement(4355..4978)
                     /locus_tag="VM910_31020"
     CDS             complement(4355..4978)
                     /locus_tag="VM910_31020"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HVI66975.1"
                     /translation="MRPFDDAMRRNIADRCDAFMRVPPAAAGELKRAAVAIALTEASE
                     TRETALLLTRRAAGLRSHRAQWALPGGRCDAGETQIEAALRELKQELGLELASGAVLG
                     MLDDYPTRSGYLITPVVVWTDLNQRIAPNPAEVASVHFIPLHLIEAENAFDFTDIPES
                     RHRVIRFHYEDRLIHAPTAAMIYQFREVLAGRDTRVADLEQPVFAWK"
     gene            complement(5019..5978)
                     /locus_tag="VM910_31025"
     CDS             complement(5019..5978)
                     /locus_tag="VM910_31025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI66976.1"
                     /translation="MRRKILILVYPQAQPIDIAGPLQAFATADDEVGGGAYEVRVTAL
                     DRGSVTLAGGLTTSASALPQRSPDTLLVVGGPGVHAARANASLVRAVRRLATDARRVC
                     SVCTGAFLLASSGLLDGREAATHWRACGQLAVEFPRVRVRADPIWVRDGRVWTSAGVT
                     AGIDLALALIEDDLGAAVAIRVARRLVVPMRRAGGQSQHSDLLALQSASQFGPLLEWM
                     AKNLSRALTTEVLADQAGMAPRSFHRHFLARTGVTPAAAVERLRLDRARTLLEMKRLS
                     LTEVSAQSGFGSADRLRRAFRRRFGVPPRNYDNRASNSQKPRS"
     gene            complement(6055..6606)
                     /locus_tag="VM910_31030"
     CDS             complement(6055..6606)
                     /locus_tag="VM910_31030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66977.1"
                     /translation="MKASAICAVGFAMMTLTMNAAPLSLQQCGFSLAASSMVLQVHFA
                     LMYLPSLFNPSLVSWIGLRGLIAAGIIAGAAGCLLTALTEQTLAIYIVELGLSGMAWN
                     FIFNGGTLLLANTYPAFGKVRAQGLNSLFVYSANLLASFSAGALMAFFGWEVVNIACL
                     PLLAVVAIALRSRPLAKAVVSSG"
     gene            complement(6603..7229)
                     /locus_tag="VM910_31035"
     CDS             complement(6603..7229)
                     /locus_tag="VM910_31035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66978.1"
                     /translation="MPSRDILVLAVGQALTGTVMSLLTSVSSLSGAYLTPSPSLSTLP
                     VTATVLGTLLMIYPASALMGRLGRRGGFMFKAGIGVVGGAVCFLGLASGNFVILVVGT
                     FLLGLFSAFGQYYRFAAIDAARSPQERTSAVAIVTGAGVVGGIAGPFLGSHFAEVFAS
                     VPYAGAFAALSFVCICLAASLCCPLISGAMRAGRHLPPRRASLSISIS"
BASE COUNT         1433 a         2402 c         2391 g         1248 t
ORIGIN      
        1 aaccgtcgcc ctggtcgccg actccgtcga cgtggtcaag gagggcgatg cgctgaagca
       61 ggtgcccgac aacaagatct acgtgatcga catgacggcc aatccgccga aactcgccgc
      121 cacgctcacc ggcggcaagc aaccgtcggg cctgagcttc agcccggacg gcaagatggc
      181 cttggtcgcc aaccgcggcg acaattccgt cagcgtgctc tcggtcaacg gcaccgacgt
      241 caagatcacg gacaccgtga gtcttcccga cagcgtcgcc catgtggtgt ttacgccgga
      301 cggcaagcgc gcgctcgccg tgcgtttccc ggcgcacaag gtctcggtgc tcgatattgc
      361 cgacggcaag gtgacctaca acaaggtcga tctggcgacg gggcaatggc cgtacaacgt
      421 cgtggtcacg cccgacagca agcttgcgct caccgcggac aatggcgcag ccggctcctc
      481 cgacggcagt gtcgatacca ccagcgtcat cgacctcgag gccaatccgc cccgcatcat
      541 cgaccgcgtg gtgatcgccg atgggccgga gggactggcg atgagcccga agggcgatct
      601 cgcggtcgcc gccatccagc gcggctccaa catgaagaag gcgttcttcc acgagaagaa
      661 cggcagcctg tcggtgctca agatcgacgg caagaaggtg accaagaccc aggacatcga
      721 actcggcggg ctgccggagg ccgtcatgtt cacgccggac ggcaagtaca tcctggcggg
      781 caacttcatg accgatgatt tctcgatcct gaaggtggac ggcaccaaag tcaccgacac
      841 cggcaagcgc ttcaaggtcc caggccatcc ggcatcggcg cgcatggggc attagagcgt
      901 gatagcttta tgtcgaatcg ccatcgcgct ctagtttagt atttgagcat gatctccgcg
      961 caaacgcgtt ccgcgtttgt cgcgtgggaa aaccggtgcc cacttttccg gatcatgctc
     1021 tagcaaccat ggaccagcga tagctgggct gcctcccgcg cgcttgcggg ggaggcaagc
     1081 tcattttcgc ggggcagccg atttccgaat tagctgccgc gcgcccattg ctgcagcgcc
     1141 tcggcgcggg cgatcaggcg cttcgcattc gcataggtcg gtacctcgac gagcgagccc
     1201 ttcagttcgg cgcgcgagcg gccctcggcg cgcgcggcct cgaaagcttc cacgatctcg
     1261 cgcgcgcggg agagctcctg ctcgctgggg gtcaggacgc cattgacgac cccggcatgc
     1321 gcgggatcga cgaggctttt cgcggcatag ccgagccggt gcgcccagcg cgtatcgcgc
     1381 tccacgcctt ctccatcgct gaacgtatag gggcaatcga ccgcgaccac gccggccgcc
     1441 acgcattcga caatgaagcg ctgccggcag taagcgagtt cggcgccgtc gggaccacgc
     1501 tccgcgccaa gatcggccgc gagatcttcc gacgccatca ggcaggccac cgttcgcggg
     1561 ctcgccttgg cgatcgcatg tgtttgcaac agcccgcgcg cgagctcgat attgggcagc
     1621 agcgcagtcg agccttccgc gatgccgtag tcgcgctcga accgcgtcac cgcctcgtcg
     1681 agacggacga tgtgggcggg ctctgcgacc ttcggcaacg cgacgatgtc gggacggcca
     1741 cgcatgacgg cggcgagatc ctccatgccg tcacgatcga gcggattgac gcgaaccgcg
     1801 accacgacac ccgccgcgcg ccaggcggca aagaggtcgg ccgccaatgc gcgcgccgcc
     1861 gggcgctggg cgggcgacgt gaaatcctcg agctcctgga tcagcacgtc ggcgccgctg
     1921 agcgccgcgg cttgcagcac cggttcattg gcgccctcca ggaacagcca ggagcgacat
     1981 agccttgtgg gacgaatccg gcgcatgtgg ccctccccgg ccgggatcag gtcacgggag
     2041 gccgcctact gaaaggtcat gtccagacat ttggacacgt cggagcccag gcccgatgag
     2101 atcgtgatgc agccgcgcac cttttgcgcc gtggtgtcgg ctacccacat cgcgtagccg
     2161 ccctgaccga agaaggagtt ggtgttcggc ggctcggtct cggtggcgtc gaaggtataa
     2221 ttggaatcgg tctcgaatac gcgcggcttc ttggcgcaac taccgtcggc ctgcacgttg
     2281 atcacttcct tgttgtcgcg cgtcagtgcc agcgagacct gacagcggaa atattccgac
     2341 gtcttgacgt tgaagacgta ggcgtaggat ttgcaggttg cggtgttgag tttgccgggg
     2401 gcaaggccgc ggctgcaggc gatcctctcg ttgtgggaga gcttgaacag ctcctcggcc
     2461 cgagcgaacg aaaccagcgt cacaaacgat agcgctatgc tcaagcaaaa tttcatgacg
     2521 tggttcatcc gaatcatccc tatgactgct tttgtagaat tggttctaaa cggaaaccgg
     2581 aagatagtcg cgaagcgcgc ggcgggaaat cacaaagtgc tgggccgcgc gtgatgcggc
     2641 ggcggaagca cgagccgagg gccttgttga cgatacgaac gcgttcgtga tttgttcaag
     2701 aaacgctggt ggagattaag acgatgacta gaaattcgat tccgacgctg ttgctggttg
     2761 ctgcgctcgg ggggctgacc acatccgcct tcgcacaagc ggccggcccg acaccgtggg
     2821 agctgaagcc cgatacgggt tacgcctacg acaaggaagg caaaaccttc gtctacaaga
     2881 tggggaccag caatgcgggg ctgttgttga aaggcgccaa gaaggtgccg aaaggcacgc
     2941 tgttcttcat cggccagaac ggacagctct atatgcgcag cgggccctat ctcgagggcg
     3001 acggcaggtt catgttcggc tcgaattgag ccggcagccg gctgccgagc catccttttc
     3061 ccccgagcgc gaattttgat tgcgcgggtc ggacctgcac gacttgcggg ggaagacgat
     3121 gatgtggggc tattgatccc cgcgcgcgct cgcctggccg gcgccggact caaacaccat
     3181 ggcgagctcc tttgcaccga ccttgttgct gttgaagtcc atccgactat cggtgactgc
     3241 aagcagcttg gccacggtgt tgtggttgat cgcgaccggg ttgcgctcgt aaccccggcg
     3301 ctggaaaaaa ttcgaagtcg gcacctgctc gcgcatcgcc agaggcatcg tcaccatgtc
     3361 aaggccggtg ccgtcgccgg tcaggttcat catctcgagt tcggccgcgg tcagcggacg
     3421 catatagccc ttctgccgcg cgaagatcac cgaagtcagt agcttgtgtg tctgcaacat
     3481 cggcccgacg tcgatatcga ggaccatggc atgcatcccc cagccctgcg gcgtgtcgcc
     3541 gatcttttcg tgcagcgacc agtcgtccgg cactgacgtc gccagcgcca ccatcttctt
     3601 gagcaccgcg atcttgtgct ccagcgcctt gcgtgcggga ccggtgaggc ctgcggctct
     3661 ctcgccaagc tccttcacga actcgtcgcc ctgctcggcc agcagttcga cggtgttggt
     3721 ggtcagcagt tgattatagc cgatcgcgct cgagatcgcg tgcgagccat ggaagcccga
     3781 ctgcgatgcg tgcgtgccgt cgccgccggt ctcgaacgca tagacccgca ccgcctgttc
     3841 gcgcgtcaat cctgcagccg aggcgtaacg cgcataggcg cgcttgaaat cgatctcgct
     3901 cgcgggccgt tgcggcgccc attggaaata ttcggaggcc gcctgcacga gatcggcgac
     3961 cacggggatc ggtctgtggg gctgtggcct ggcgtgctcc ggcagaggct ccggcgatgg
     4021 gcccggcggc cgcgcgggcc cggtatagac cggcggctgt gtcagcacgt agtcatcaag
     4081 cgtaatctgc tgctgattgc gtcgcttggt attgcgggcg cgccgcttat cggcaatcga
     4141 ggtccagtag gcgtcccttt ccgcttcaaa ggcctcgcgc gccgccagat attccttcca
     4201 cgtggcagcc tgcggcgaag cctccgcctc ggcgacggcc atcggcagca cgtcgcccgc
     4261 gcgcgtcgag gcgaacggca acaggagcgc caggatggtc aagtgacggc gaatcgcttt
     4321 ggtgagcatt ccgcacttgt acccgaaaac acggctactt ccaggcaaac accggctgtt
     4381 ccagatcggc gacgcgggta tcgcgcccgg ccagcacttc gcggaattga tagatcatgg
     4441 cagccgttgg cgcgtggatc agccggtctt cgtagtgaaa gcggatcacg cggtgcctgc
     4501 tctccgggat atcggtgaaa tcgaacgcgt tttccgcctc gatcagatgc agcgggatga
     4561 agtgcaccga ggcgacttcg gccgggttcg gcgcaatcct ctggttgaga tcggtccaca
     4621 ccacgaccgg cgtgatcaga taacccgagc gggttggata atcgtcgagc atgccgagca
     4681 cggcgccaga ggcaagctca agaccgagtt cttgcttcag ctcgcgcagc gccgcctcga
     4741 tttgcgtctc gccggcatcg cagcgaccgc ccggcagcgc ccattgcgcg cgatgggacc
     4801 gcaggcctgc ggcgcggcgt gtgagcagca gcgccgtttc cctggtttcg ctcgcttccg
     4861 taagggcgat cgcgaccgcg gcgcgcttca gctcgcccgc cgccgcaggc ggcacgcgca
     4921 tgaacgcgtc acagcggtcg gcgatattcc gccgcatggc atcgtcgaaa ggtctcatga
     4981 cgctcgaatg actgccgtgg acttccacgc ttcactactc aacttcgagg tttttggcta
     5041 ttggaagccc gattgtcata gttacgcggc gggacgccaa agcggcgcct gaaggcccgc
     5101 cgcaaccgat cggccgatcc gaaccccgat tgtgccgata cctccgtaag cgacagccgc
     5161 ttcatctcga gaagcgtgcg cgcgcgatcc agccgcaggc gctcgaccgc tgcagcgggc
     5221 gtgaccccgg ttcgcgccag gaaatgacga tgaaagctgc gaggggccat gccggcctgg
     5281 tcggccagga cttccgtggt gagtgcgcgc gacaaattct tcgccatcca ctccagcagc
     5341 ggtccgaatt gcgaagcgct ctgcaacgcg agcaggtcgc tgtgctgcga ttgcccgccc
     5401 gcgcgtcgca tcggcaccac gaggcgacgc gcgacgcgga tcgcaaccgc cgcaccaaga
     5461 tcatcctcga tcagcgccag cgccagatcg atgccggccg tgaccccggc agacgtccac
     5521 accctgccgt cccgcaccca gatcgggtcc gcccgcaccc gcacgcgcgg gaactccact
     5581 gcgagttgac cgcaggcgcg ccagtgcgtt gccgcttcgc ggccgtcgag caagcccgat
     5641 gacgccagaa ggaaggcgcc cgtgcacacg gagcaaacgc gacgcgcatc ggtggcaagc
     5701 cgacgaaccg ctctgacaag cgacgcattt gcgcgcgcgg catgaacgcc gggaccgcct
     5761 accaccagca gggtgtcggg cgatctctgc gggagtgcag acgcggatgt ggtcagaccg
     5821 ccggcgagcg tgacggagcc gcgatcgagc gccgtcaccc gcacctcgta agcgccgccg
     5881 ccgacttcat catcggcggt ggcgaacgcc tgaagcgggc cggcgatgtc gatcggctgc
     5941 gcctgcggat agaccaggat caggatcttg cgtcgcattg gcgtatcctg ccggatcggc
     6001 acgctatctg ccaacagttt cctgcgcgaa gcccgtggcc ttgcgcggga acgatcagcc
     6061 ggacgaaacc actgctttcg ccagcggccg cgatcgcagc gcgatggcga cgacagcaag
     6121 cagcggcaaa caggcaatat tgacaacttc ccagccgaag aacgccatga gcgcaccggc
     6181 cgagaacgat gccagaagat tggcgctgta aacgaacagc gagttcaagc cctgggcccg
     6241 caccttcccg aacgccgggt aggtgttggc aagcagaagc gttccaccat tgaagatgaa
     6301 attccatgcc atccccgaca acccaagctc gacgatatag atggcgagtg tctgttcggt
     6361 cagagccgtc agcaggcacc cggccgcgcc ggcaatgatc ccggccgcga tcagccctcg
     6421 caatccgatc catgacacca ggctcggatt gaacaaagac ggcaggtaca tcagggcgaa
     6481 atggacctgg agcaccatcg acgatgctgc caatgagaag ccgcattgtt gcagcgagag
     6541 cggggcggca ttcatggtca gggtcatcat cgcgaagccg acggcgcaga tcgcgctcgc
     6601 tttcatgaaa tcgatattga gagtgaagcg cggcgcggcg gcaagtgccg gcccgcccgc
     6661 atcgcgcccg agatcagcgg acagcagaga gacgccgcca gacagatgca gacgaacgac
     6721 agcgccgcga atgcaccggc atacggcacc gaggcgaaga cttcagcgaa atggctgcca
     6781 aggaatggac cggcaatgcc gccgaccaca cctgcgcccg tcacgatcgc aaccgccgag
     6841 gtgcgttcct gcgggcttcg cgcggcgtcg atggcggcga aacggtagta ctggccgaaa
     6901 gcgctgaaaa ggccgagcag aaaggtgccg acgacgagga tgacgaaatt tccgctcgca
     6961 aggccgagaa agcagaccgc accaccgacg acgccgatgc ccgccttgaa catgaaccca
     7021 ccgcggcggc caaggcggcc catcagcgcg gacgccgggt aaatcatcag cagcgtgccg
     7081 agcacggtcg ccgtcacggg caacgtgctg agcgagggag acggcgtcag ataggctccc
     7141 gacaggctcg ataccgaggt gagaagcgac atgaccgttc ccgtcaaggc ctgcccgacg
     7201 gccagcacca ggatatctct cgagggcatt gcagatgact ccgatgttgc cgttgaacgg
     7261 ccgcggccgg ccgagccgtg cccgcccctc cgctttcggg aaatggagag tcgtgagcgg
     7321 acccgatttg tcccaattcg cgctttggca gcaatgacat ttatccggca ttttctgcca
     7381 aagccggtgt cagccgatcg cctgcggcgg ccttcatcgc ttgactaggc caggcaagca
     7441 gccgatgaaa tgacggcgca ttcaggagtg gacg
//
LOCUS       DATLJT010001352         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1030547,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001352 DATLJT010000000
VERSION     DATLJT010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030547"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VM910_31040"
     CDS             complement(<1..459)
                     /locus_tag="VM910_31040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66979.1"
                     /translation="MLLAAENLSATCKFDSMFKSRLQIAAEALVIVALSASAAAENPQ
                     APIGTLPDLEAALQACWVPPPMEQSWPGMQITVLMSFKRNGELFGEPRITFQSREASE
                     AERSAYRIAVAQALKRCASLSFTEALGNAVAGQPLTMIFIDDREQSPDSSR"
     gene            735..1145
                     /locus_tag="VM910_31045"
     CDS             735..1145
                     /locus_tag="VM910_31045"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HVI66980.1"
                     /translation="MLRLASRPTPVLAWLLATAYAAHAGEATDAASAAADQFKLMVGS
                     ALMTDYIYRGLSYSAHQPSVGTYVDAQQGWRYVYTNFNSVRFSTSPAVEVTMAVGARP
                     TLGPFEFDIGAAYYYYPGELGPELSNYWEAHATV"
     gene            1293..1772
                     /locus_tag="VM910_31050"
     CDS             1293..1772
                     /locus_tag="VM910_31050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66981.1"
                     /translation="MSLDVGRWLFGPTSSGGGVSAAGGGLPLPDFTNWRAGLTFTYRV
                     FKLGLNYTDTNLSKENCYVLTGDLGALPGGVANPGPNPFGFALGPVRRDVLGNAWFRH
                     QSCDARPLTTSCASRFSARTPIGRDGFAAPTHRPRCERGRLRAGRFAPEIQSGTFGC"
     gene            complement(2147..>2593)
                     /locus_tag="VM910_31055"
     CDS             complement(2147..>2593)
                     /locus_tag="VM910_31055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HVI66982.1"
                     /translation="ISVDAIFNEFRGRFIGKASPVHFFWGTFDLAVTRFSGRRAPEIP
                     GADRITREAYSHEVSSVGWWPGDAMVKEPAFFSYAAPQPSGFSKAPVRPAAASYNTDL
                     LQFLLRYDDVRGAASPRQALLDFCQSTYEAAATLGKWNRAELEHSA"
BASE COUNT          467 a          849 c          794 g          483 t
ORIGIN      
        1 tcttgatgag tcaggggatt gttcccgatc atcgatgaag atcatggtga gcggctggcc
       61 ggccacggcg tttccgagcg cctccgtaaa cgacaaagac gcacatcgtt tgagcgcttg
      121 cgccacggcg atgcgatatg ccgagcgttc cgcctccgac gcctccctcg attgaaatgt
      181 gatccttggc tctccgaata gctcgccgtt gcgcttaaag ctcatcagca ccgtgatctg
      241 cattcctggc cacgactgct ccatcggcgg cggaacccag catgcctgca gcgccgcctc
      301 gagatccggg agcgtaccga tgggcgcttg cgggttctca gcggcggcgc ttgccgaaag
      361 cgccacgatc accaacgcct cagcggcaat ttgcaagcga gatttgaaca tcgagtcaaa
      421 cttacaggtg gcagacaaat tttccgctgc aaggagcatc gagcaatacc tgcgagcggt
      481 cggccgtgat gtagctcggc accggggttt tcgctggggg cgtgttgctc gcagccggct
      541 cgcatagaca ggcgcggata aggtaactgg cgcaaacgat ggagacaacg cgccgccagc
      601 agtcagcagc aacgctcccc gctgcatctt ttcacccgca ttcgtactga ctgtggcgga
      661 cggatcacgg tctactgggt ggttgtgaga tagcttacga cgcttgagcc gcgccaaccg
      721 caacgaagcc cgccatgctg cgtcttgcct cgcgcccaac gcctgtcctc gcctggcttc
      781 tcgccacggc gtacgccgcg cacgccggcg aggcgacgga tgccgcgagc gcagcggcgg
      841 accagttcaa gctcatggtc ggctcggcgc tgatgaccga ttacatctat cgcgggctca
      901 gctattcggc gcaccagccg tcggtcggga cctatgtcga cgcccaacag ggctggcgct
      961 acgtttatac gaacttcaac agcgtgaggt tttcgaccag cccggccgtc gaggtgacga
     1021 tggcggtcgg cgcgcgcccg acgctcggtc cgttcgagtt cgatatcggc gccgcctatt
     1081 actattatcc cggcgagctc gggccggagc tgtccaacta ctgggaggcg cacgccacgg
     1141 tatagtacaa gctgaccgac aagatcacgc tggcaccgac cctcgcctac gcgcccaacg
     1201 tctggcagac cggcgcctcg ggcacctatg cagccggcac gctctcgttc gacctgccga
     1261 gcgagctcct tcccgccgac gtgggctggg cgttgtccct cgacgtcggg cgctggctgt
     1321 tcggtcccac ctcgagcggc ggcggcgtga gcgcggcagg cggcgggctt ccgctgccgg
     1381 actttaccaa ctggcgcgcc gggctgacgt tcacctaccg cgtgttcaag ctcggcctca
     1441 attacaccga cacgaacctc tcgaaagaga actgctacgt gctgaccggc gacctcgggg
     1501 cgctaccggg cggcgtggcc aatccgggcc ccaatccctt cggatttgcg ctcgggcctg
     1561 tgcggcgcga cgttctcggc aacgcttggt ttcgacatca atcctgcgac gctcggccgc
     1621 tgacaacgag ttgcgcgtct cggttttccg caaggacccc gatcggccgc gatgggttcg
     1681 ccgcgccaac ccatcgtccc agatgcgagc gcggccggct ccgcgccggc cgcttcgcgc
     1741 ccgaaataca atcagggact ttcggttgct gatggcaggt tgtctcgatt agtaacaccc
     1801 gaatgacgag ctcgccgggg acactggcgc cgcgttcaca ggctgggtaa acgtcgggac
     1861 cgcgatcaac gcggccagcg cgagagcagc gataagcgat ttcatgacac gtctccatgt
     1921 tggactattc gtcctagatt tgcgcagcga cgaggccact cggccagcgc ctacgtgagg
     1981 gaacatgtag gctccggtcc cgccgcgctg tgatagccat cacatcttca ggacacttca
     2041 gcgtgattca gatcttttgc gcgctcacct cggcgtgatg atctgtcgtt aaatcattga
     2101 tgcagccgca tcgcgcgcga catctgtgcc cgtggatcac gccccgctag gcgctgtgtt
     2161 ccagttccgc gcgattccat ttgccgagcg tcgccgcggc ttcgtaggtg ctctggcaga
     2221 agtcgagcag ggcttgccgc ggcgaagcgg cgcctcgcac gtcgtcatat ctcagtagga
     2281 actgcaagag atccgtattg taggacgccg ccgccgggcg aacgggcgct tttgaaaatc
     2341 ctgatggctg cggcgcagcg taggaaaaga aggccggctc cttcaccatc gcgtctcccg
     2401 gccaccagcc cacgctgctc acctcgtgcg aataggcctc ccgcgtgatc cgatcggcgc
     2461 cgggaatttc cggcgcgcgg cggccggaaa agcgcgtcac cgccaagtcg aaggtgcccc
     2521 agaagaaatg caccgggctg gccttgccga taaaacgtcc gcgaaattca ttgaaaatcg
     2581 catccaccga aat
//
LOCUS       DATLJT010001353         3111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2180463,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001353 DATLJT010000000
VERSION     DATLJT010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180463"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..649)
                     /locus_tag="VM910_31060"
     CDS             complement(206..649)
                     /locus_tag="VM910_31060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66983.1"
                     /translation="MRYLVFALALLGATATSARAQDAIPDLKGTWSGKGKSIVFGTNP
                     HHPGSQTPADPPRVRDIEATYVVDGQEGRLAWGRSWSTAANTQEPFAWAIANDNKTIV
                     GADMDGYFRITLISPDSIEKCYTHNGTSPTHAIVATCHVMNRVKR"
     gene            1230..1454
                     /locus_tag="VM910_31065"
     CDS             1230..1454
                     /locus_tag="VM910_31065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66984.1"
                     /translation="MSTNDDVQKRNAFAACAEFRIEGLEASLQHAREIAVREELGVVG
                     HYIAEAQGYLAQIRLLHQEALDEFSRAQGE"
     gene            complement(1658..1731)
                     /locus_tag="VM910_31070"
     tRNA            complement(1658..1731)
                     /locus_tag="VM910_31070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1697..1699),aa:Gln,seq:ctg)
     gene            complement(1866..>3111)
                     /locus_tag="VM910_31075"
     CDS             complement(1866..>3111)
                     /locus_tag="VM910_31075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI66985.1"
                     /translation="SRPKTTMAQAAELLTPARASETKLVAGVCFAHLVSHYYMLMLAP
                     LFAFIRADFGVTYTELSLALTTFNVVSAVSQTPVGFFIDRTGARINLIGGLLLGSAAI
                     AIAGAAHSFWVFIAMFAVLGLANTVYHPADYTLLSERVSPRRMTQVFSFHTCAGMIGS
                     AIAPVSLLFMQSAVGWRGAFVCSALLGVVAALVLAVQGEPPVVHPAHPSKPRGGEAAS
                     AASDGWQLLLSPPILLNLLFFVVLSMVGGGLNQYLVVGLGALYGTPPALANTALTGLL
                     TMSAIGVLLGGALASRTVRHNLVACTGLVVTGSASTFIAFVDPGALLLVLIASLSGFA
                     SGMTMPSRDMIVRSVTPPGSFGKVFGFVTTGFHIGGMVAPLIFAQFLDHGYPRGVFLY
                     VAACALVAIATVAFGMSGRRTA"
BASE COUNT          643 a          955 c          993 g          520 t
ORIGIN      
        1 aacgatcgat ttatatcggc gggaaccaaa ccgctcgccg aacgataacc ttgtggcgtt
       61 cccggaaggc cccactgccc cccaagagcc aacgggagcg ctaaggcccc ggtgaaaacc
      121 tcgccggggc cttcctcatg acgctattgg cgctggccgt cacgacctgg gcgcgccgct
      181 actttcgcgg caggcgcgac aagtcctacc gtttcacgcg attcatcacg tggcaggtgg
      241 cgacgatcgc atgggtcggg ctggtgccgt tgtgggtgta gcacttttca atactatccg
      301 gcgagatgag ggtgatgcga aaatagccgt ccatatcggc gccgacgatg gtcttgttgt
      361 cgttcgcaat cgcccacgcg aacggctctt gggtattggc ggccgtggac cacgagcgac
      421 cccacgcaag tcggccctct tggccatcca cgacgtaagt cgcctcgatg tcgcgcacgc
      481 gcggcggatc ggcaggggtc tgcgaaccgg gatgatgggg attggtgccg aacacgatcg
      541 acttgccctt tcccgaccac gtgcccttga gatcgggaat ggcgtcctgt gcgcgcgcgg
      601 acgttgctgt ggcgccgaga agcgccaacg cgaagacaag atatctcatc acttcctccc
      661 gtgctgatgt ggatcgctca tcgaggtgat tggctgacgg cccgcgcgcg agtaatgacc
      721 caatatcagc agcggcccaa tgaaaacgtc accggtgcgg ccattcgaat cgccgacgcg
      781 ctcaattgtt ggctcagcaa gggtcaaaaa ccgccacatc tgctccgatg cggcgcgcgg
      841 gtcttccgcg ccgagttaat gaattgccgc cgtctcatgc gctccccaag ccgaggccac
      901 tgtgggttga gagttctatc acatgccaga cgcgttgtgc gttacagccc ggcaatgtcc
      961 gagttaggtc aaaaaaccaa cccgtgccgt cacttgccgg gacctcgggc gcggagcggc
     1021 agccttccgc cggttcgaag gcattcagcc cggttcagaa aaattcagat ctagcccaag
     1081 gacctttaag gcaactctgg agaattattc cagcagccca aggcaggtca cgtcagcaat
     1141 ccgccgaggc cgtggcgatc cggaggggca aaacaaaaag attgacgccc cgctcaacgc
     1201 caggttccgg caaatttggg gatagagcca tgtcgaccaa cgacgacgtt cagaagcgaa
     1261 atgcctttgc cgcttgtgct gagtttcgca tcgagggcct tgaagcctct ctgcagcatg
     1321 cgcgcgagat tgcagtccgc gaagagctgg gtgtcgtagg acactacatt gcagaagcgc
     1381 aaggatacct ggctcaaatt cgcctcttgc atcaagaggc gctggatgag ttcagccgag
     1441 cgcaggggga ataagcctgt tgggcggtcc gccgaccgat gtgcctcctg tttgctgcgc
     1501 tttggatagg cttctacgta gctcactgcg atcagggacg gcaccggttc gcccacctaa
     1561 ccgccacagg acagaccgcg ccgcatctcg ctgacacgcc ggaatgtcgg gatgattttt
     1621 tcagagttag gcaggcgcca atggagacca aactggctgg ctgggggact aggattcgaa
     1681 cctagattaa cggagtcaga gtccgctgtt ctaccattga actatccccc aatcgggaaa
     1741 agcaatcctc gagtgtaacc ggtcccgtcg gctcgttgcg ttgtcccggt cggcgtaggg
     1801 ctcgaacgtc gacacatcta ggacatcttg cgcgcctttc caagccgcaa cagcgcggac
     1861 gccgctcacg ccgtgcgccg gcccgacatg ccgaatgcca cggtggcgat ggccaccagc
     1921 gcacaggcgg cgacgtagag aaataccccg cgcgggtacc catgatcgag gaactgcgcg
     1981 aagatcaagg gcgccaccat gccgccgata tgaaagccgg tggtgacgaa gccgaacacc
     2041 ttgccgaacg agcccggcgg ggtgaccgag cgcacgatca tgtcgcgcga cggcatagtc
     2101 atgccgctgg cgaaaccgct cagcgacgcg atcaggacca acagcagcgc gcccggatcg
     2161 acgaacgcga tgaacgtgct ggcgctgccc gtcaccacca atcccgtgca cgccacgagg
     2221 ttgtgccgta cggtgcggct ggcgagcgct ccgccgagca agaccccgat ggcgctcatg
     2281 gtcagaagtc cggtgagcgc ggtattggcg agcgcgggcg gcgttccgta gagcgcgccg
     2341 agcccgacca ccagatattg gttgagaccg ccgccgacca tcgagagcac gacgaagaag
     2401 aggaggttga gcaggatcgg cggtgacagc agaagctgcc agccgtcgct ggccgcgctc
     2461 gctgcctctc cgccgcgcgg cttcgacgga tgtgccggat gaacgaccgg cggctctccc
     2521 tgcaccgcca gaacgagggc cgcgacgacc ccgagcagcg ccgagcagac aaaagcgccg
     2581 cgccatccca ccgcgctctg catgaatagc aggctcaccg gcgcgatggc tgaaccgatc
     2641 atgccggcgc aggtgtggaa cgagaacacc tgggtcatgc ggcgcggcga gacgcgctcg
     2701 gacaataagg tgtagtcggc gggatggtag accgtattgg cgaggcccaa gacggcgaac
     2761 atcgcgatga acacccagaa cgaatgcgcc gctcccgcga tcgcgatcgc ggcggagccc
     2821 aacagcaggc cgccaatcaa gttgatgcgc gcgccggtgc ggtcgatgaa gaagccgacc
     2881 ggcgtctgcg acaccgccga gaccacgttg aaggtggtga gcgcgaggct cagctcggta
     2941 taggtcacgc cgaagtccgc gcgtatgaaa gcgaacagcg gcgcaagcat cagcatgtaa
     3001 tagtggctga ccaggtgggc gaagcacacg ccggcgacga gcttggtctc gctcgcgcgg
     3061 gcgggggtga gaagctcggc agcttgggcc attgttgttt ttggtcgtga c
//
LOCUS       DATLJT010001354         4154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2641750,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001354 DATLJT010000000
VERSION     DATLJT010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641750"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..381)
                     /locus_tag="VM910_31080"
     CDS             complement(88..381)
                     /locus_tag="VM910_31080"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI66986.1"
                     /translation="MQSASFAMVLPMITSSQCRAARGLLDWTQQELADRAGVGIVTVR
                     HFEGGLNEPRRATLEVIRRALEAAGVEFIQQNGGGPGVRLRKRHRARRGGAHR"
     gene            465..1292
                     /locus_tag="VM910_31085"
     CDS             465..1292
                     /locus_tag="VM910_31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HVI66987.1"
                     /translation="MGQSTAVIYSNRQCFIGGSDARIIMGRDGAALLRLWREKRGEVG
                     PEDLSGNLIVQLGKVTEDLNRSWYERNTGRHVTDVQRRVRHSAIPWMVATLDGIVEAT
                     GAVFESKFMLPWSFSEETAAEKYMAQLQHNMWVTHLRASVLSIITGGGKWVEITIPMD
                     PLYLSVLVSAEKKFWRCVQSGEPPHLINAEPPRPLIEAIRIVDMSSSNSWAEFAALFR
                     NTRSAFLDHERAKSELKALMPEDAKEAIGHGVKARRSKSGAVSFDVLEMEVTHAPVQ"
     gene            1318..2505
                     /locus_tag="VM910_31090"
     CDS             1318..2505
                     /locus_tag="VM910_31090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HVI66988.1"
                     /translation="MAKAQAELINPEKSLTATIRTGRPGDGERSFRYAPLSSGLDIVR
                     KTLGQHEIAILQTTAIDQTAGMVNLTTTLAHASGEWFASDWPVCPIAETANPQRMGAA
                     LTYARRYALFTLVGIAGEDDLDAPDNCVPSPVTGASTTGPSTDGLRFPPQTHGNGKIR
                     GAAKTASRTVLLTDESTALRDRLLSEIAGLASQDSAATWAGEALPAKNTLTTADAKLL
                     EDAFERKLSQLSELPAETVESPIIEAQPAAVEGSQGINRGTTVNQPKGIDKSVLAVSA
                     PRRYRNKEHLRYVTLQPCLLCDRKPSDAHHLRFVQPRALGRKASDEFAVPLCRIHHRE
                     AHRASNERAWWKAAGIDPLKIARKLWNDTRANKSQDRSNLSSEAARTDRPFTSDGIDS
                     KAQ"
     gene            2508..3950
                     /locus_tag="VM910_31095"
     CDS             2508..3950
                     /locus_tag="VM910_31095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66989.1"
                     /translation="MAFPLALPCPAPCLFHDPNGTAFADLTIEGHRETWPIRSRRFRH
                     WLRRRYYEETGSAASAETIRSAVDLLEARAQFDAPERTVHVRVAEHDGRTYLDLADKD
                     WRAVQIGPDGWQVVTSPPVRFRRAAGMLPLPIPQRGGSIEALAAFLNLPAPDEFVLVV
                     AWLLAALQRGGPYPVLVIAGEQGAAKTMLTKILRALLDPNAAPTRAPPREERDLMIAA
                     NNGHILAFDNLSSLPSWLSDALCRLASGGSFAIRQLYTDCDEVLFQAARPAILNGIED
                     VVCRSDLADRALFLALEPIADERRRTESQLWLEFELVRPRLLGALFDAAAHGLRRLSR
                     VRLERSPRMADFALWATACETALWPPGTFLRAYDANRRAAIEGAIEADPVAAFVREIM
                     AVRSTWAGRASDLLRARIAASEGAPDTTAGWPRNPRALAGRLRRCQTFLRTVGIDIAF
                     GREGRTGSRMIRMTSLAKSPRISVRNGREA"
BASE COUNT          813 a         1299 c         1242 g          800 t
ORIGIN      
        1 cggtccacag ccggacatag ccagcgcccg gcgttgctcg tgcgcaatct ttatcgaacg
       61 agatgaattg agaattctat tgcgccttta gcggtgcgca cctccgcgcc tagcccgatg
      121 acgctttcgg agccgcacgc ccgggcctcc gccgttttgc tgaataaatt cgacgccggc
      181 ggcttccaga gcgcgcctaa ttacttccag agttgctcta cgaggttcat tcaatccgcc
      241 ctcgaagtga cgaacggtca cgatgccgac tccagctcga tcggcaagct cttgttgtgt
      301 ccagtcgagg agtccgcgcg ctgccctgca ttgtgacgag gtgatcatcg gcagaaccat
      361 ggcaaaagac gccgattgca tcaaatctat ctaaaatcga ttgacattct gaaatatcta
      421 ttttagatat ttatgccgta attagataga caaggattgt gcccatggga cagtcaacgg
      481 cagtcattta ctcaaacagg caatgcttta ttggaggctc cgacgcgcgg atcatcatgg
      541 ggcgcgacgg ggctgccctc ctgcgcctct ggcgcgagaa gcggggagaa gtcggcccgg
      601 aggacctatc cggcaacctc atcgtgcagc tcggaaaggt gaccgaggac ctcaaccgca
      661 gctggtatga gcgcaacacc ggccggcacg tcaccgacgt ccaacgccgg gttaggcact
      721 cggccatccc ctggatggtg gcgaccctcg acggcatcgt agaggcgacg ggagccgtct
      781 tcgaatccaa gttcatgctg ccctggtcgt tttccgagga gacggccgcc gaaaagtata
      841 tggcccagct ccagcacaac atgtgggtca cccatttgag ggcttcggtg ctgtcaatca
      901 tcaccggcgg tggcaagtgg gtggagatca caattcccat ggacccgctc tatctcagcg
      961 tgctggtctc ggcggaaaag aagttctggc gctgcgtgca atccggcgaa ccgccccatc
     1021 tcatcaatgc cgagccgccg cggccgctca tcgaggccat tcgcatcgtc gacatgagtt
     1081 catcgaactc gtgggccgag ttcgccgcgc tcttccgcaa tacccggagt gcatttctcg
     1141 accacgaacg ggccaaaagc gagctcaaag ccttgatgcc cgaggatgcc aaggaggcca
     1201 ttgggcacgg cgtgaaagcc aggcgctcga aatcgggggc ggtgagcttt gatgtgctgg
     1261 agatggaggt gactcatgcc ccggtccagt gaaacggtcg cggcgctggc ctcggcgttg
     1321 gccaaggccc aggcagaact catcaatccg gaaaagtcgc tcacggccac catccgaacc
     1381 ggccggccag gagacggcga acgcagcttc cgctacgcgc cactctcaag cgggctcgac
     1441 atcgtgcgca agacgctggg gcagcacgag attgccatct tgcagacgac cgccatcgat
     1501 cagaccgccg gcatggtcaa tttgaccacc acgctcgccc acgcctccgg cgagtggttc
     1561 gcttcggatt ggccggtctg ccccattgcc gaaaccgcca atccccagcg catgggcgcg
     1621 gcgctgacct atgcccgccg ctatgctctc ttcacactgg ttggcatcgc cggcgaagat
     1681 gatcttgatg cgcccgacaa ctgcgtgccg tcgccggtga ccggggcgtc aacgacaggg
     1741 ccgtcgacag atggattgcg gttccctcca caaacacacg gaaacggcaa gattcgtggc
     1801 gcggcaaaga ctgcgagccg aacggttctt ctcaccgatg aatccactgc tcttcgcgac
     1861 cgtcttctga gcgagatcgc cgggctcgct tcgcaggaca gtgctgccac ctgggcaggc
     1921 gaggcgcttc ctgccaagaa cacactaacc acggctgacg caaagctgtt ggaagacgcg
     1981 ttcgagcgga aactatccca gttgtcggaa ttgccagctg aaaccgtcga gtcgccaatt
     2041 atcgaggcgc agccagctgc ggttgaaggc tctcagggca tcaacagggg cacaactgtc
     2101 aatcaaccca aggggatcga caagagcgtg ctcgcggtaa gcgcgccacg tcgctaccgc
     2161 aataaggagc atcttcgcta cgtcaccctc cagccatgcc tcctgtgcga ccgcaagcct
     2221 tccgatgcgc atcacctgcg gttcgtgcaa ccgcgcgcgc ttggtcgtaa ggcgagtgat
     2281 gaattcgcgg taccgctctg ccgtatccat caccgcgagg ctcatcgcgc tagcaatgag
     2341 cgggcctggt ggaaggccgc cggcatcgat ccgctcaaga ttgcgcgcaa actttggaac
     2401 gacacgcgtg caaacaagag tcaggatcgg tcgaatctgt catcggaggc ggctcgaacc
     2461 gatcgaccat tcacttccga tggtatcgac agcaaagcac aataacgatg gcctttcccc
     2521 ttgcgcttcc ctgcccggcc ccatgtttgt ttcacgaccc gaacggcacc gccttcgccg
     2581 atctcacgat cgagggccat cgtgaaacgt ggcctattcg cagcaggcgg tttcggcatt
     2641 ggctacgacg tcgatactat gaggagaccg gtagcgccgc gagcgcggag acaattaggt
     2701 cggcggtcga tctgctcgag gcgcgcgccc agtttgatgc cccggagcgc acggttcacg
     2761 tccgggtcgc tgaacacgac ggccgcacct atctcgatct tgccgacaaa gattggcgag
     2821 ccgtccagat agggccggac ggatggcagg tcgtcacctc tccaccggtt cggttccgtc
     2881 gtgccgccgg aatgctgccg cttcctattc cgcagagagg cggatcgatc gaagccctcg
     2941 cagcattcct caatcttcca gcaccagatg aattcgtgct ggtggtggcg tggctgttgg
     3001 ccgccctgca gcggggcggt ccctatccag tgttggtgat cgctggcgag cagggggcgg
     3061 caaagaccat gctcacgaaa atcctgcgcg cgctgcttga tcccaatgca gctccaacgc
     3121 gagcgcctcc acgcgaagag cgcgacctca tgattgccgc caacaatggc catatcctcg
     3181 ccttcgacaa tttgtccagc ttgccctcct ggctctccga tgccctttgc cgactcgcca
     3241 gtgggggtag cttcgccatc cgccagcttt ataccgactg tgatgaggtg ctcttccaag
     3301 ccgctcgccc ggccatcctc aacggcattg aagatgtcgt ttgccgctca gatctggctg
     3361 accgagcact ctttcttgcc ttggagccca tagcggatga gcgccgccgc acggagagcc
     3421 agctttggct cgagttcgaa ttagtacggc cgcggctcct cggcgctctg tttgacgctg
     3481 cggcccatgg gctgcggagg ttgtctcgcg tccgccttga acgctcgccg cgcatggctg
     3541 atttcgcgct gtgggcaact gcctgtgaaa cggcgctttg gccgcctggc acctttttgc
     3601 gcgcctatga tgccaatcgt cgggctgcga tcgagggcgc cattgaggcc gaccccgtag
     3661 ccgcttttgt gcgggagatc atggccgtgc gcagcacatg ggcagggagg gcctccgatc
     3721 ttctgcgcgc ccgtattgcc gcaagtgagg gtgcgccgga cacaaccgca ggatggccaa
     3781 gaaatccgcg cgcactggct ggtcgattgc gtcgctgcca gacgttcctg aggaccgtcg
     3841 gcattgatat tgccttcggt cgcgaaggcc ggacgggcag ccggatgatc agaatgacct
     3901 cgctggcaaa gtctccgcgc atcagcgtac gcaacggtcg ggaggcttga gtgagggggc
     3961 ccgtcactga cgcaactgac gcaaatgaca caaccgacga attgcgggct tatgccgagc
     4021 tgagattgcc cttatgtcgc gaagggtcga aaaggcaact ggagcatcca ccgcgtgcgt
     4081 gagcggttgg tcagtcagcg caccggcatc atcaatcaga tccgcgcctt cctattggag
     4141 cgcggtgttg ccgt
//
LOCUS       DATLJT010001355         1509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_111690,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001355 DATLJT010000000
VERSION     DATLJT010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111690"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VM910_31100"
     CDS             complement(<1..1306)
                     /locus_tag="VM910_31100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66990.1"
                     /translation="MRALYALVVPVLLASSGFVTAGYIDDNTTFSLAISVLRGAVGPH
                     ARALRIEADVNGIEIHAQDAHNRSHINGWRYGTVTYMGVPLRRLTGPEPIDPALVNPD
                     IEANLFDLDSIDFAATPKLIHDSIARANLQDPAAVTRMEIARRSFILPQPTGGEVRWT
                     VHVDSGREQADIFADAQGAIVGADVSGTQRAKTLNIPNEPGLAAEAAAAFRDGFSGDR
                     VLTNVAIEKKTMSFSTNIGDKSLGKLVTNMPVTATFTRDLNGLQRRLGAIDINATMGA
                     PPPAAFAIADVDWAILGKLEERAIDKAAVLHGLGNRVSVAISADQLGGPVLIWAVEVT
                     DPTGEVTTIIADAKGAITRVILPESRRPKIDWRQPAALAGAIGRVEAIFGGTAKIASI
                     VADNQQARITVEDPAHGGQPATFEFSADGVSRAAIVFSLDSMG"
BASE COUNT          287 a          476 c          455 g          291 t
ORIGIN      
        1 gtcccatcga atcgagcgag aagacaattg ccgcgcgact gacgccatcg gccgaaaatt
       61 cgaacgtcgc cggctgtccg ccatgcgcag gatcctcgac cgtgatgcga gcttgttggt
      121 tatcagcgac gatcgacgca atcttcgcgg tcccgccgaa aatcgcttcc actcgcccaa
      181 tcgcgccggc aagcgctgcc ggctgccgcc aatcgatttt gggacggcgc gactccggca
      241 atatgacgcg ggtaatggcc cccttcgcat cagcaatgat cgtggtcacc tcgccggtcg
      301 gatcggtcac ctcgacagcc cagatcagaa caggtccacc tagctgatcc gcggagattg
      361 caacactgac acgattgccc agtccatgga ggacggcggc tttgtcgatc gcgcgctctt
      421 cgagttttcc gaggatcgcc caatcgacgt cggcaatagc aaaagcggcg ggcggcggcg
      481 cacccatcgt cgcattaata tcgatcgcgc cgaggcgtcg ctgcaaacca ttgaggtccc
      541 gggtgaaagt cgccgtcacc ggcatgttgg tgaccagctt gccgagggac ttgtcgccga
      601 tgtttgtgct gaaggacatc gtctttttct cgatggcgac gttggtcaga acacgatcgc
      661 cgctaaaacc gtcgcgaaac gccgcggccg cttcggccgc aaggccgggc tcgttcggga
      721 tattgagcgt tttggcgcgc tgggtgccgc tgacgtcagc gccaacgata gcgccttgcg
      781 catcggcaaa aatatcggcc tgctcgcggc cgctgtcgac atgcacggtc catcgcacct
      841 ccccgccggt cggctggggc aagatgaaac tgcgtcgcgc gatctccatc ctcgtgacgg
      901 ccgctggatc ctgcaaattc gcgcgcgcga tcgagtcatg aattagtttc ggcgtggcgg
      961 cgaaatcgat cgagtcgaga tcaaataaat tggcttcgat atcgggattg acgagggccg
     1021 gatcgatcgg ctccggcccg gttaagcgcc gtaatggcac gcccatgtaa gtaacggttc
     1081 cgtagcgcca accgttgatg tgactgcgat tgtgtgcgtc ttgcgcatgg atttcgatgc
     1141 cattgacgtc ggcttctatc cgcaatgcac gtgcatgcgg accgaccgcg ccgcgcaata
     1201 ccgagatcgc caggctaaag gtcgtattgt cgtcgatata gcccgccgtc acgaagccgc
     1261 tcgatgcaag cagcaccggg acgaccagcg cgtaaagtgc cctcatccgc tactccatct
     1321 gcgctggccg tcaggcggca agccgaggcg cgccccgggt tagtctggtg tgctcaattc
     1381 ccggcccggc aatcgcgcaa aatttccggc ctgaaggcaa gaccgtggcc ggggcgttcc
     1441 ggggcggtga tcacgccctt caccaccggc accggctcga cccagatatc gtccgcgagt
     1501 cccatgtct
//
LOCUS       DATLJT010001356         1857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1722670,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001356 DATLJT010000000
VERSION     DATLJT010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722670"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VM910_31105"
     CDS             complement(<1..500)
                     /locus_tag="VM910_31105"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66991.1"
                     /translation="MKRRAFISLLGGAAAGWPLAARAQERMRHIGVLMGYAESDREGQ
                     ANFAAFQEGLQKLGWTEGRNIRIDTRWASADADLVQRFAKELVALQPDLIVTQNTPIT
                     AAMLQRTRTIPIIFANVSDPVGSGFVAGLPRPGGNVTGFIDMEGSMAGKWLELLKEVA
                     PRVTRVA"
     gene            complement(497..1501)
                     /locus_tag="VM910_31110"
     CDS             complement(497..1501)
                     /locus_tag="VM910_31110"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI66992.1"
                     /translation="MQFGQLQRREFITLVGYAAAWPLAARAQQRERMRRVGVLVSSLA
                     ADDPEWQARGNAFVQGLQERGWADGRNVRIEYRWGLGDQDRQRKYAAELVALAPDVIL
                     AAGPPAVASLQQSNRSVPIVFANVSDPVGNAFVASLARPGGNTTGFMQFEYGLSAKWL
                     ELLKEIAPRVTRAAVLRDPAISSGLGQFGAIQAVAPSLGLDVSPVDVRDVRAIERAVG
                     AIARQPNGGLIVTGSASAVIHRDLIITLAAQHKLPAVYFQRHFVASGGLISYGADLID
                     EYRLAAGYVDRILKGEKPADLPVQNPTKYELVINLKTAKALGLDVPPTLLARADEVME
                     "
BASE COUNT          357 a          607 c          555 g          338 t
ORIGIN      
        1 gcgaccctgg tgacgcgcgg cgcgacctcc tttagcagct ccagccactt gccggccatc
       61 gagccctcca tatcgatgaa gccggtgacg ttgccgcccg gtcgcggcag gcccgcgacg
      121 aagccgctgc cgaccggatc ggaaacgttc gcgaaaatga tggggatggt gcgcgttcgt
      181 tgcagcatcg ccgcagtgat gggtgtgttt tgcgtaacaa tgaggtcggg ctgtagcgcg
      241 acgagttcct tcgcaaatcg ttgcaccaaa tccgcgtcgg ccgacgccca gcgcgtgtcg
      301 atccggatgt tgcggccctc cgtccacccg agcttttgga gtccctcctg gaacgccgcg
      361 aaattggcct gtccttcccg gtcgctctca gcatacccca tcagcacgcc aatgtgccgc
      421 atccgctcct gcgcacgcgc cgcgagtggc cagccagccg ccgcgccgcc aagaagcgat
      481 atgaacgcgc gtcgcttcat tccatcactt cgtcggcgcg agcgagcagc gtcggtggca
      541 cgtcgaggcc gagcgctttg gcggtcttga ggttgatcac cagctcatac ttggtggggt
      601 tctgcaccgg cagatcagcc ggcttctcgc ccttgagaat gcgatcgacg tagccggccg
      661 cgagccggta ctcgtctatc aagtcagccc cgtaagagat caagccaccg ctggcgacga
      721 aatgacgttg gaaatagacc gcaggtagct tgtgctgcgc cgcaagtgta atgatcagat
      781 cgcgatgaat aaccgccgat gcgctcccgg tcacgatcag gccgccgttt ggctgacgcg
      841 cgatggcccc gacggcgcgc tcgattgcac gcacatcgcg cacgtcgacc gggctcacgt
      901 ccagtcccag cgatggcgcc acagcctgaa tggcgccgaa ctggccgagc ccagaggata
      961 ttgcgggatc gcggaggacc gccgcgcgcg tcacgcgcgg cgcgatctct ttgagcagtt
     1021 ccagccactt cgcgctcaat ccatattcga attgcatgaa gccggtggtg ttgccgcccg
     1081 gccgcgccag gctcgcgacg aaggcgttgc caaccgggtc gctaacattc gcgaacacaa
     1141 taggcaccga acggtttgat tgttgtaacg acgccaccgc ggggggcccc gcagccaaga
     1201 ttacgtccgg cgcgagcgca accaattccg ccgcgtattt tcgctgtcga tcttgatcgc
     1261 ctaaacccca gcgatactcg atacgcacgt ttcggccgtc agcccagcct cgctcctgca
     1321 ggccttggac gaaggcgttg ccgcgagcct gccattccgg atcgtccgca gccaagctgc
     1381 tcaccagcac cccgacacgc cgcatccgct cgcgctgctg cgctcgcgct gcaagcggcc
     1441 aagccgccgc gtagccaaca agggtgatga actcacgcct ttgcagttga ccgaattgca
     1501 taggccacct ctgcgtccgt gacagcataa cggattgacg aggatcaagt caggggcttg
     1561 ctgcaatgcg ggatttcgac tggattaccg ccgcttcggg tcagtctcgg tcattcggcg
     1621 atgttggcct caatgtccgg tttgcccgaa agcggactca agtcggacat ctcccgaggt
     1681 ccgagaagtg ccaattccgg actcctggaa cgcagcaaac tgtttgcgag ggaccatctc
     1741 cggaaaaacc gatgcacaac gcccaaggca ggcctggaaa gtgttgcgga ggtggatatc
     1801 tatcaacagg caacccgccg ccaaagaaag atgaagccgt atttgtcgaa taccgcc
//
LOCUS       DATLJT010001357         1870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_111956,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001357 DATLJT010000000
VERSION     DATLJT010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111956"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VM910_31115"
     CDS             complement(<1..567)
                     /locus_tag="VM910_31115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66993.1"
                     /translation="MRRLLLAMMTLAAVISMAAPVSAQTVGVVLMHGNTDTPYGTIAS
                     LATALEGAGYPVERPEMCWSYRRRRDRPLLDCLSELEAPIERLSGRGVRAIVVAGMSV
                     GGLGAIAFGARRQGLVGIIALAANGSPERLVRLFPQIAQSVAEAQAMVASGRGDERAS
                     FTDGNIRGLFPINTTAAIYLSFFDPTGPA"
     gene            892..1236
                     /locus_tag="VM910_31120"
     CDS             892..1236
                     /locus_tag="VM910_31120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI66994.1"
                     /translation="MHFHANYVSTSVAGDYYQAMFEAEQDSDDLDSPYLLIQRQFEDP
                     DDESCYIETHDQKYCGHFLLRRVDFTPQKLSIELDRPRDKFVSVTFAMVATEFAEASQ
                     VVKIISGAIEPP"
     gene            complement(1386..1520)
                     /locus_tag="VM910_31125"
                     /pseudo
     CDS             complement(1386..1520)
                     /locus_tag="VM910_31125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
     gene            1721..>1870
                     /locus_tag="VM910_31130"
     CDS             1721..>1870
                     /locus_tag="VM910_31130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI66995.1"
                     /translation="MKKLSGKTALVTGSTDGVGRLVARKLGQAGARVLVHGRDAERGA
                     RVVADI"
BASE COUNT          374 a          587 c          564 g          345 t
ORIGIN      
        1 ggcgggtccc gtcggatcga aaaagctcag atagatcgcc gccgtcgtat tgatcgggaa
       61 cagtccgcgg atatttccgt cggtaaagct ggcgcgttcg tcgccgcggc cggaggcgac
      121 catcgcctgc gcctcggcga cgctctgcgc gatctgggga aaaagacgca ccaggcgctc
      181 cggcgacccg ttcgcggcga gcgcgatgat gccgaccaga ccctgccgcc gcgcgccgaa
      241 ggcgatcgct ccgagcccgc ccacactcat accggcgacg acgatggcgc ggaccccgcg
      301 accgctcagc cgttcgatcg gcgcctccag ttcgctcagg cagtcgagaa gcgggcggtc
      361 acgcctgcgc cgatacgacc agcacatctc aggacgctcg accggatagc cagcaccctc
      421 caatgccgtg gcgagcgagg cgatagtccc atacggggtg tcggtgttgc catgcatcag
      481 gaccacgccg acggtctgcg ccgagacagg cgcggccatg ctgatgactg ctgcgagtgt
      541 catcatcgcc aggaggagtc gcctcatgtc caacctcgct agaccgaatc aaacggattg
      601 taccctgcgc gcttaagtcc atctggaaat ccgctctcgg cgcaacctgg ccggtcagcc
      661 atgcttcgac gaaggtcggg tttccaccat cacggccgct cgccgtagtt gtcgcgaagg
      721 acagcgtcgc gtcacgatgg atcgaaggcc caccgacgct tccggtcaac ccgtacaacc
      781 aagccacgcc gtccgccgct gtgcgccgtg gggaaacctc ggtgacgctg tttcaaatca
      841 accgggaccg cgataggctt tcttgacccg gccgtgtagt tgaggggaag catgcacttt
      901 cacgccaact atgtctccac ttctgttgcg ggcgactact accaagccat gttcgaagct
      961 gagcaagatt cggatgacct tgacagcccc tatttattga tccaaaggca attcgaagat
     1021 ccagatgacg agtcttgcta tatcgaaacg catgatcaaa aatattgtgg tcattttcta
     1081 ttacgccgcg tcgatttcac gccgcagaag ttgtcaatcg agctcgatcg tccgagggac
     1141 aaatttgtca gcgtgacctt tgcgatggtg gccacggagt tcgccgaagc atcacaggtg
     1201 gtaaagatca tcagtggcgc gatcgagcct ccctgatgcg cccgcagacc aaatcaaatg
     1261 tccggatcgg gtgaatggcg gcggttgctg ccccgagcag cagagtcagc cttaccccag
     1321 tgagccgacc accttccact cggagtcaat catctcttgt gtgccaattg ccgacatctg
     1381 ccgagcccga gggcaccgcc aacgacgacg aggcttgcct gcatgatgca gccccgcttg
     1441 taggccggct tccactcggc gaggagccag cgatcgtcaa gttgaaggcg cgccggctgc
     1501 tcagcgatgt taatgtagat agcagctcga cgatgtcgag gacttccgtg ctgtcccgac
     1561 gcccttcacg ccggcttgtg atggacacgc cttggcgccg ataactgggc cgatcgtcgt
     1621 gcgcggcgcc aaacctggcg atgtcgtctc aatcgaatga cttcgagctc gcgggatccc
     1681 aaagtaatct ctttcgtcga gccacgtgag gagacctgag atgaaaaagc tgagcggaaa
     1741 gacggcactg gtcaccggct cgacagacgg ggtcggccgg ctcgtcgctc gcaagctcgg
     1801 gcaagccggc gcgcgcgtgc tggtgcatgg ccgtgatgcg gagagaggtg ctcgcgtcgt
     1861 tgccgacatc
//
LOCUS       DATLJT010001358         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2870595,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001358 DATLJT010000000
VERSION     DATLJT010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870595"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VM910_31135"
     CDS             <1..662
                     /locus_tag="VM910_31135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HVI66996.1"
                     /translation="MGGRIGYSAATGVMVILLSWLGIISVLLALVPVVAILPILLYIG
                     MLIGAQAFQTTPVKHAPAIVLALTPHLAAWAKLLIDTGMASMTNAAQAVGGLSPEKMA
                     EVKAAAIAALPQQGLVYHGLEVMGGGSILTGLVLGAIGVFVIERDFIKASAFALAGAV
                     LTYFGFMHGEAVGMGGGLGVSPAVALSYAVVAIGLFALGRLGTSEHYASHPEMSAAPA
                     E"
     gene            complement(738..1517)
                     /gene="soxA"
                     /locus_tag="VM910_31140"
     CDS             complement(738..1517)
                     /gene="soxA"
                     /locus_tag="VM910_31140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HVI66997.1"
                     /translation="MNLLRAIAVATLLFSATPLLAADIPPNERRSGYSFMAEDTKTIQ
                     DDDTANPGMLWVLDGEALWNRKTGNAGKACAECHGDGRESMKGVAARYPAFDKSLGRP
                     VDLEQRINLERTQRQGAPPLPYESRDLLALTAFIANQSRGMAISAGDDPQLKPFVEKG
                     RELFTLREGQLNLACANCHDDNWDKHLAGSAITQAHPTGYPEYRLEWQTLGSLQRRLR
                     SCMTGIRAQAYDYGAPELVELELYLMSRARGMPIETPAVRP"
     gene            complement(1514..1834)
                     /gene="soxZ"
                     /locus_tag="VM910_31145"
     CDS             complement(1514..1834)
                     /gene="soxZ"
                     /locus_tag="VM910_31145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HVI66998.1"
                     /translation="MTAALINVPRKARRGDIIEIKTLMSHVMEPGYRRTAAGDLLPRD
                     IITSFACRYNGEEIFRADFFPAIAANPFLSFFTAAKESGKFEFEWTGDNGFSETASSS
                     ITVE"
     gene            complement(1834..2313)
                     /locus_tag="VM910_31150"
     CDS             complement(1834..2313)
                     /locus_tag="VM910_31150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HVI66999.1"
                     /translation="MRHPPTTTRRKFLGLAGSAAVAGAVPIATMRSVDATPAILNAAI
                     RNLVGEAPVHAGKVKLDIPPLVENGNTVPMTVSVASPMTVEDHVKSIHVFNEKNPQPN
                     VGNYYIGPRAGRAQISTRIRLADSQKVVAIAQLSDGSFWSATADVVVTLAACTDEVI"
     gene            complement(2330..2782)
                     /gene="soxX"
                     /locus_tag="VM910_31155"
     CDS             complement(2330..2782)
                     /gene="soxX"
                     /locus_tag="VM910_31155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HVI67000.1"
                     /translation="MARSRAHIAWFFAALFALPFGAHAQALAPYRIVGDGIPDSLTGS
                     PGDAERGRALVLDRTSTCILCHSGPFPETRFQGDLATGLAGAGNRWSEAQLRLRLVDA
                     AHFNPATIMPSYYRVDGLARVGTNWRGKPILSALQIEDIVAYLATLRE"
     gene            complement(2775..4052)
                     /locus_tag="VM910_31160"
     CDS             complement(2775..4052)
                     /locus_tag="VM910_31160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HVI67001.1"
                     /translation="MDALRPPTRRDAIFGMTASAASLAFPSVLRAQASARVLVVGGGF
                     GGTACARALKRSDATLEVTLLEPNRTFVACPFSNEVIAGMRDIAAQQFGYDRIVAGGI
                     SVIPQEATKIDPLARNVMLGDRTSLSYDKLVLSPGIDLRFDTLEGYDEAASRPMPHAW
                     KAGEQTLLLRRQIEAMDDGGLVAIAVPANPSRCPPAPYERASLIAHYLKTKKSRSKVL
                     ILDAKDSFPQQRLFESAWKELYGDMIERVALSQGGRVTSVDPKTMTIVTEFGNYSAEV
                     ANVIPPQKAGAISGIAGAADHTGWCPIDPTTFESKLVPDVHVIGDACLGGGIPKSAAA
                     ASAQGKACAAAIINLLSGKPPEMPKLTGVCYNTVAPGYGFSLAGIYQPKDDIFAEVEG
                     GGTSPVDAPRSLREREAGDAQNWFQTITADSFG"
BASE COUNT          704 a         1304 c         1393 g          796 t
ORIGIN      
        1 cgatgggcgg acggatcggc tattccgccg caaccggcgt catggtgatc ctgttgtcct
       61 ggctcggcat catctcggtg ctgctcgcac tcgtgcctgt cgtcgcgatc ctgccgatcc
      121 tgctctatat cggcatgctg atcggcgcac aggcgttcca gaccacgccg gtcaagcacg
      181 cgcccgcgat cgtgctggcg ctgacaccgc atcttgcggc ctgggcgaaa cttctgatcg
      241 acaccggcat ggcttcgatg acgaatgccg cgcaggcggt cggcgggctc tcgcccgaga
      301 agatggccga ggtgaaggcg gcggcgatcg cggccctgcc gcagcagggc ctggtctatc
      361 acggcctgga agtcatgggc ggcggctcga tcctgaccgg tctcgtgctc ggcgcgatcg
      421 gcgtgttcgt catcgagcgt gacttcatca aggcgtcggc gtttgcgctg gccggcgcgg
      481 tgctgaccta tttcggcttc atgcacggcg aggctgttgg tatgggcggc ggtctcggtg
      541 tgagcccggc ggtcgcgttg tcctatgcgg tggtcgcaat cgggctgttc gcgctcggca
      601 ggctcggcac cagcgagcat tatgcctcgc atcccgaaat gtcggcggcg ccggcggagt
      661 agggcggctt ccgccattca aggcttcgaa cgggccgcac atggcggccc gttctgcgtc
      721 ttctaaagcg cgatggctta aggccttacc gccggcgtct cgatcggcat gccccttgcc
      781 cgcgacatta gataaagttc gagctcgacc agttccggcg cgccgtagtc gtaggcctgc
      841 gcccggatgc cggtcatgca actgcgcagg cggcgctgca gcgagccgag cgtttgccac
      901 tccagccggt actcgggata gccggtcggg tgtgcctggg tgattgcgga ccccgcaaga
      961 tgcttgtccc aattgtcgtc gtggcagttg gcgcaggcca gattgagctg cccttcgcgc
     1021 agcgtgaaaa gttcgcggcc cttctcgaca aacggcttga gctgggggtc gtcgccggcg
     1081 gaaatcgcca tcccgcgcga ctggttggcg atgaaggcgg tgagcgcgag cagatcgcgg
     1141 ctctcgtaag gcagtggtgg cgcgccctga cgctgcgtgc gctccagatt gatgcgctgc
     1201 tcgagatcga cgggacggcc gagcgacttg tcgaaggccg gatagcgcgc agcgacgccc
     1261 ttcatgcttt cgcgcccatc gccgtggcac tcggcgcagg cctttccggc attgccggtc
     1321 ttcctgttcc acagcgcttc accgtcgagc acccagagca tgccgggatt agccgtgtcg
     1381 tcgtcctgaa tcgtcttggt atcctcggcc atgaaactgt agccggaccg gcgctcgttc
     1441 ggcgggatgt cggccgcgag caggggcgtt gcggaaaaca gcagtgtcgc gaccgctatc
     1501 gcgcggagca aattcattcg accgtgatcg aggaagacgc ggtttccgaa aatccgttgt
     1561 cgccggtcca ttcgaattcg aacttgccgc tctctttcgc ggccgtgaaa aacgacagga
     1621 atgggttggc ggcaatcgcc ggaaagaaat cggcccggaa aatctcctcg ccattgtaac
     1681 ggcaggcgaa gctcgtgatg atatcgcgcg gcagaaggtc gccggccgcg gtgcggcgat
     1741 agcccggctc catgacgtgc gacatcagcg tcttgatctc gatgatgtcg ccacgcctgg
     1801 ccttgcgcgg aacgttgatc agggccgcgg tcatcagatc acctcgtccg tgcaggccgc
     1861 gagcgtcacc acgacgtcgg cggtcgccga ccagaacgat ccatccgaca actgcgcgat
     1921 cgccacgacc ttctgactgt cggccagtcg aatccgggtc gaaatctgcg cccggccggc
     1981 gcgcggcccg atgtagtaat tgccgacatt gggctgcggg ttcttttcgt taaagacgtg
     2041 aatgctcttg acgtgatcct ccaccgtcat cgggcttgcg acgctgaccg tcatcggcac
     2101 ggtgttgccg ttctcgacca gcggcggaat atcgagcttg accttgccgg cgtgcaccgg
     2161 cgcctcgcca accaggttgc ggatcgcggc gttgagtatc gcgggcgttg cgtccaccga
     2221 acgcatggtc gcgatgggga ccgcgcctgc aacggcggcg ctgcctgcga ggccaaggaa
     2281 cttgcggcgc gtggtcgttg gtgggtggcg cattcgcatt tatgtcgtcc tattcacgaa
     2341 gcgttgcaag gtaggccacg atatcttcga tctgcaaagc ggagaggatc ggcttgccgc
     2401 gccagttcgt cccgactcgc gcaagaccgt ccacgcgata ataggagggc atgatcgtgg
     2461 ccggattgaa atgcgcggcg tcgaccagcc gcagccgaag ctgggcctcg ctccagcggt
     2521 tgcccgcgcc cgcgaggccg gtcgcaaggt cgccctggaa cctggtttcg ggaaaaggtc
     2581 cggagtggca gagaatgcag gtgctcgtgc gatcgagcac cagcgcgcgg ccgcgctccg
     2641 catctcccgg cgaacccgtg agcgagtcgg gaatgccgtc gccgacgatt cgataggggg
     2701 caagcgcctg tgcgtgcgcg ccaaatggca gcgcaaataa cgcggcgaaa aaccaggcga
     2761 tgtgagcgcg cgatctagcc aaagctgtcc gccgtgattg tttgaaacca gttttgcgcg
     2821 tcgccggctt cgcgctccct caagctccgg ggcgcatcga ccgggcttgt gccgccgcct
     2881 tcgacctcgg caaagatgtc gtcctttggc tgataaatgc cggcaagcga aaagccgtag
     2941 ccgggagcga ccgtgttgta acagacgcca gtgagcttag gcatctccgg cggcttgccg
     3001 gaaagcaggt tgatgatcgc cgccgcacag gccttgccct gtgcggacgc tgcggcagcc
     3061 gattttggaa tgccgccgcc caggcaagcg tcgccgatga catggacatc gggcacgagc
     3121 ttcgattcaa atgtcgtggg atcgatcgga caccagccgg tatggtcggc cgcgccggcg
     3181 atgcctgaga tcgcaccggc cttttgcggt ggaatcacat ttgcgacctc ggcgctgtaa
     3241 ttgccgaatt cggtgacgat cgtcatggtc tttggatcga cggaggtgac gcggccgccc
     3301 tgcgacagcg ccacgcgttc gatcatgtcg ccgtaaagct ccttccaggc actctcgaac
     3361 aggcgctgct gcggaaagct gtccttggcg tcgaggatca ggaccttcga gcgcgacttt
     3421 tttgttttga gataatgcgc gatcaggctt gcgcgctcgt agggcgccgg cggacagcgc
     3481 gaggggttcg cgggcaccgc aatcgcaacg agacccccat cgtccatcgc ttcgatttgc
     3541 ctgcgcagca acagcgtctg ctcgccggct ttccaggcat gcggcatcgg gcgcgacgcc
     3601 gcttcgtcat agccttcgag ggtgtcgaag cgcagatcga tgccgggcga aagcacgagc
     3661 ttgtcgtaag agagcgatgt acgatcgcca agcatgacat tgcgcgccag cggatcgatc
     3721 ttggtcgctt cctgcgggat cacactgatc ccgcccgcaa cgatccggtc gtagccaaat
     3781 tgttgtgccg cgatgtcgcg catgcccgcg atcacctcgt tcgagaaggg gcaagcgacg
     3841 aatgtccggt tcggttcgag cagcgtcacc tcgagcgtcg catcgcttcg cttcagggca
     3901 cgcgcgcagg ctgtgccgcc aaagccgccg ccgacgacga gaacgcgtgc gctcgcttgc
     3961 gcgcgcagaa ccgacggaaa ggccagcgat gccgcggacg cggtcatgcc aaatatggcg
     4021 tctcgccgtg tcggcgggcg taacgcgtcc atcaacatga tccggaactg tgcggcggcc
     4081 ttttcaggcc gccgcctgct tcgtctcagg caaacgtgat gttctgctcc cgcaagggaa
     4141 cagatcggat gcgcttgccg gtcgctgcga aataggcgtt gagcaccgcg ggtgccg
//
LOCUS       DATLJT010001359         1854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4479274,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001359 DATLJT010000000
VERSION     DATLJT010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4479274"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1854
                     /locus_tag="VM910_31165"
     CDS             <1..>1854
                     /locus_tag="VM910_31165"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI67002.1"
                     /translation="SGSPGELDPVFRAVLENATRLCDAKFGTLNLYHGEIFRNVALYN
                     VPSAYLETELHEVIRPHPKSGHAHIVRTKQVLHIEDLTAMPPYREGDPAATALADLGG
                     ARTIVVVPMLKEDELIGTIGIYRQEVRPFTQKQIELVSNFAKQAVIAIENTRLLNELR
                     ESLQQQTATADVLKVISRSAFDLQAVLDTLVESAARLCEAQTANIFRPKDGFYEIVAS
                     YGFSSAHYEYMQRHPIPISPGTVTGQAALEGRTIHVHDVLAEPDYKLIEAQRIGGYRT
                     VLCVPLLREGMPIGVIALSRPVVRPFTDKHIELVTTFADQAVIAIENVRLFDEVQART
                     RDLSEALERQTATSEVLQVVSSSPGELKSVFDAMLRNAVRICGARYGMLSLCKGEEAR
                     SVAMYGVEPALVEKLQGKLRRPGPNTAAGRLMRTKTAVHIADVRTEPGFFDTPPGFTG
                     PELAIHAGARTLLAVPMLKESELVGYITIYRQEPEPFTEKHIELVTNFAAQAVIAIEN
                     TRLLNELRESLQQQTATADVLKVISRSTFDLKSVLQTLVESAARLCDADNAAITRQQG
                     GMFFRAEAYGYSPEYIEYIRTVPVEPERGSVMGRALLERRVIHVPDVLADP"
BASE COUNT          395 a          554 c          548 g          357 t
ORIGIN      
        1 cagcggctcg cctggcgagc tcgatcccgt attccgggca gtgctggaga acgccacgcg
       61 gctctgtgac gccaagttcg gcacgctcaa cctataccac ggggagatat tccgaaacgt
      121 cgcgctctac aatgtgccgt cggcatactt ggaaacggaa ttgcacgagg tgatccgtcc
      181 tcatccaaaa tccggtcatg ctcacattgt ccggaccaag caggtcctac acatcgaaga
      241 cctaacagcg atgccacctt atcgcgaagg cgatccggcg gccaccgcgc ttgccgacct
      301 gggcggtgcg cgcacgatcg tggtcgttcc aatgctcaag gaggacgagc tgattggtac
      361 catcggcatt taccgccagg aggtacgacc atttacgcag aagcaaattg aacttgtcag
      421 caatttcgcg aagcaagccg tcatcgccat cgagaacacg cggctgctca acgagcttcg
      481 cgaatctctg cagcagcaga ccgccaccgc cgacgtgctc aaggtgatca gccgctcggc
      541 gtttgatcta caggccgtgc tggacacgct ggtcgagtcg gcggcgcggc tgtgcgaggc
      601 gcaaacggca aacattttcc gtccgaaaga cggattttac gaaatcgtcg ccagctacgg
      661 cttctcgtcc gcccactacg agtacatgca acgtcacccg attccgataa gtccaggaac
      721 ggtcaccggg caagcggcgc tggaaggcag aacaattcat gttcatgatg ttctcgctga
      781 gccggattac aaattgatcg aggcccagag gattggcgga tatcgcacgg tgctttgcgt
      841 cccgctgttg cgggaaggaa tgccaatagg cgtcattgcc ctgtcacggc cagtcgtgcg
      901 gccattcacc gacaagcaca tcgagctagt gacgaccttc gcagaccagg cggtcattgc
      961 catcgagaac gtgcggctat tcgacgaggt gcaggcgcgc acgcgtgacc tttccgaggc
     1021 gctggaacgg cagacggcga cctcggaggt gcttcaggtt gtttcaagct cgcccggcga
     1081 gcttaagtcc gtgttcgacg cgatgctgcg aaacgcggtg cgtatctgtg gcgctaggta
     1141 tggcatgttg tctctctgta agggagaaga agctcggtca gtcgcgatgt acggcgtaga
     1201 gcccgccctt gtcgagaaac tgcagggcaa actgcgtcgt cccggaccca acacagccgc
     1261 tggtcgcctg atgcgtacga aaacggcggt tcacatcgcc gatgtgcgaa cggaaccggg
     1321 tttcttcgat acgccgccag gattcaccgg cccggagttg gcgatacacg ctggtgcgcg
     1381 gaccttgtta gccgttccga tgctgaaaga gagcgagctg gtcggctata tcaccatcta
     1441 tcggcaggaa ccggagccgt tcaccgaaaa gcacattgaa ctggttacga acttcgccgc
     1501 ccaggccgtt atcgccatcg agaatacgcg cctgctcaac gagctgcgcg aatcgctgca
     1561 gcagcagacc gccaccgctg acgtgctcaa ggtcatcagc cgctcgacct tcgatctcaa
     1621 gtcggtgctg caaacgctcg ttgaatcggc tgcccggctt tgcgacgccg ataacgccgc
     1681 aataacccgg cagcagggcg gaatgttctt tcgcgctgag gcctatgggt actcgcccga
     1741 atatatcgaa tacatcagaa ccgttccggt cgaacctgaa cgcgggagcg tcatggggcg
     1801 agccttgctg gaacgacggg ttattcatgt ccccgacgtg cttgccgatc cgga
//
LOCUS       DATLJT010001360         7993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1031296,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001360 DATLJT010000000
VERSION     DATLJT010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031296"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VM910_31170"
     CDS             complement(<1..575)
                     /locus_tag="VM910_31170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67003.1"
                     /translation="MRVSIITGGGSGIGAAVARRLAAGDQCLMLHGQGADAAGRERLE
                     AVAGECRRAGASVEWRASDLATEGTATELVRAARATFGAVDAVVHAAGFADRRGFLEL
                     PREGLQRSFAVMAAAFHELIGAAMADLARGANGRVVAISSFVAHRFLADATYPASAAA
                     KAALEALVKCLAIELAPSGATANIVMPGYTR"
     gene            839..1636
                     /locus_tag="VM910_31175"
     CDS             839..1636
                     /locus_tag="VM910_31175"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67004.1"
                     /translation="MSADAAEAFWRQLLTEGHRPLRMTHLLFRHLPSPPRCKVCHNPF
                     DGFGGRLVGLFGFTRSRKNPNLCARCCDAMPPGGAEVDIAVLFADVRGSTAMAEKLGP
                     SAFAARMNRFYLTATEVLIRHDAIIDKLVGDEVMALFIPGICGPSYRRRAAEAAFALL
                     RTVGEQLSPASHVPIGVAVNSGLAYVGNVGSQDVVDFTALGDTVNTAARLASSAAAGE
                     VLLSETIHAALGETYADLDKRTLALRGKAAPVTVRAARPCPPPASSA"
     gene            complement(1587..1868)
                     /locus_tag="VM910_31180"
     CDS             complement(1587..1868)
                     /locus_tag="VM910_31180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67005.1"
                     /translation="MNDYDPLRDYLKTQTRPELVLSFEEIEEIIGGSLPRAAQRASWW
                     DSLRSPQERMPQREACLAAGYVATRMPDGRSVRFRRMKQGAGTAAPRAR"
     gene            2018..3514
                     /locus_tag="VM910_31185"
     CDS             2018..3514
                     /locus_tag="VM910_31185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVI67006.1"
                     /translation="MRFGGRKDTAKQLWRQSAKLLIACSVATVIGFSAICASVMLDMR
                     HGEEELARQSLENLAIGIEADISRNIELYDLSLRNVASNIVAPDIKNVSRPVLHLILF
                     DHAATANHFGAIQVFDAEGRLTVDASTLDPLPENRAGEDYFTVHRDQPDVGLYINRPM
                     LHRGSYAIVLSRRISGPDGRFLGVVAGSIRFSYFHELFGRLNLSPGDIITVLRRDRTI
                     IMRTPFDLDVIGKQVRERRDGIVLTAQPSGWFSDVSPVDSTYRLYVWSGGLKPLVVVV
                     GKPWDSILRLWRIELIRIGSIMLTLIMFVLAATLFLAREIDRRALAEDKLEELATTDA
                     LTGLKNRRKFDATIESEWRRAMRYRTQLSLLMIDADHFKAFNDTYGHPAGDQVLVGIA
                     ICISDSVKRAGDCAARYGGEEFAVLLPGQSEREALGIAETIRAKVQEWSDDPAITTVS
                     IGIASVTPTAALDWPQLVHAADQALYAAKAAGRNCCALAKLPDLLLAA"
     gene            complement(3583..4464)
                     /locus_tag="VM910_31190"
     CDS             complement(3583..4464)
                     /locus_tag="VM910_31190"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HVI67007.1"
                     /translation="MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDKPLIQYVVDEA
                     KEAGIEHFIFVTGRNKGVIEDHFDRMFELDATLAARGKKTEQQILARDQPEAGAMSFT
                     RQQAPLGLGHAVWCARDIVGDEPFAVVLPDELVLHTPGCLAQMVAAAKHLPEKSNLLA
                     VQEVPADQTHQYGICGVGKRHAKANMFEVDGMVEKPAKGKAPSNFSITGRYILQPEIF
                     AILSKHERGAGDEIQLTDAMIGLAKKQKFYGVEFAGERHDCGSKAGFLRANIAFGMAR
                     SDLRDGLRADMKRYSGK"
     gene            4629..6011
                     /locus_tag="VM910_31195"
     CDS             4629..6011
                     /locus_tag="VM910_31195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI67008.1"
                     /translation="MRQPGRPKALTAVAMVAAGLLMAGEARAQSSGGGLSTLFSNIFS
                     GPKSDNQAPPQGAPAATGSIPWSGEDGASGHPLMTAAAIRQAAANFGNCVGAMWPDAA
                     RRNVSRENFERFTAGLTPDLRIMDLMDAQPEFTKSVWDYLDILVNDNRLAKGKEILAK
                     YKAQFDATEKAYGVDRYIIAAIWGIESNYSTQIGDRSVLQSTATLACIGRRQGYFKEE
                     FLSALEILNRGDLRPEQMHGSWAGAFGPTQFMPTAFKRYAVDADGDGRRDVVDDAPDL
                     IASTANNLKKDGWQSGQSWGYEVTVPQGFNYMLADRAKVMTAAQWEHLGLKRANGQPF
                     PHPADKGYLLAPAGAEGPGFLMLPNFRVIMKYNPAEAYALAIGHFADRLRGSQPFVQP
                     WPRQERELSRAERLELQQLLVQRGFYRGTPDGQFGGETRQALRNFQASIGAPADGFAS
                     AQMLDRLRGN"
     gene            6157..7872
                     /locus_tag="VM910_31200"
     CDS             6157..7872
                     /locus_tag="VM910_31200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HVI67009.1"
                     /translation="MFKRGLLLKVLTEPGPSIVLLVVIALLVGIVGPASAEFFNFGNL
                     GGGWGRPASRSGGGWFGSDFFTPFQQQAPKRVYQDFSKAPPPEKREAVPERNVMVMGD
                     GMADWLASGLEDAYSEQPDMGVIRKAKSSSGLIKYQPKGDPADWTAAAKSILAAEKAD
                     IIVVMLGLNDRVAMREPAGEKTEKPAEKKSDKKDARAKGDAKGDAKAAEPKSDKAETA
                     VKPEEKAVDTELPPDDADNAEAPPAIVPGKSTRAPNGLYEFREDRWVELYSKKIEVMI
                     AVLKSKGVPVVWVGLPAIRGPKGSQDMLFLDSLYREAAAKAGITYVDVWDGFVDERGL
                     FLQKGPDFEGQIRQLRSYDGVYFTKPGARKLAHYVEREISRLIAARSAPITLPTEPAT
                     PDANAVPGQPAPRPLAGPVLPLVASNVGTDQLLGGPGTRPAAVDALAARTLVKGEPLT
                     PPPGRADDFTLPRREVGYEPAKGDTPVASTTPGSGATPGGGATPATPGVAALPQQRQQ
                     PPLQQRRPLQQNPAQQNPFGNFFGFGNSSPRQWSPQPRPFTPGILRPPAPIGRSAAAS
                     SNPTR"
     gene            complement(7874..>7993)
                     /locus_tag="VM910_31205"
     CDS             complement(7874..>7993)
                     /locus_tag="VM910_31205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67010.1"
                     /translation="PLRRLGTPSDMAGAALFLASPLSSYIIGQTIVVDGGLIL"
BASE COUNT         1440 a         2673 c         2506 g         1374 t
ORIGIN      
        1 tttcgcgtat agccgggcat cacgatgttg gcggtcgcgc ccgacggcgc aagctcgatc
       61 gcaagacatt tcacgagggc ttccagcgcg gcctttgccg ctgcggaggc cggataggtt
      121 gcatcggcca gaaagcgatg cgcgacaaag ctcgagatcg ccacgacccg gccgttcgcg
      181 ccgcgcgcca ggtccgccat cgctgcgccg atcagctcgt gaaacgccgc ggccatcacc
      241 gcgaacgagc gctgcagtcc ttcgcgcggc agctcgagga aaccgcgccg atccgcaaaa
      301 cctgcggcgt gcacgacggc atcgaccgcg ccgaacgtgg cgcgcgcggc acggacgagt
      361 tcggtggccg tgccttccgt cgcaagatcg ctcgcacgcc attcgacgct cgccccggcg
      421 cgccgacatt ccccggcgac tgcttccaat cgctcgcgcc ccgccgcatc ggcaccctgc
      481 ccgtgcagca tcaggcactg atcgcccgcc gccagccggc gcgccaccgc tgcaccgatc
      541 ccggacccgc cgccggtgat gattgagact cgcatcgtcg ttctgctaag tgcccctgag
      601 cgagcgacta taggtcccgc ggtttgaaat gccaacaacg ccacgcggca ccgtgcgcgc
      661 cgcgaaattc gatcaccagg aacgtctcgc gagcggacaa cgcttccgct ccagcggcca
      721 aatcaagcgc gccaccgcgc cactctctgg ccgcgcaccg acgggagggt ctatactcga
      781 aaaatcggac gcgttgcctt ggatccggac cgggcctgtc acgtcagaaa agcctgagat
      841 gtccgccgat gctgctgagg cgttttggcg ccagctcctg accgaggggc accggccact
      901 gcgcatgacc cacctgttgt tccggcatct gcccagcccg ccgcggtgca aggtctgcca
      961 caacccgttc gacggcttcg gcgggcggct ggtcggattg ttcggattca cgcggtcgcg
     1021 caagaatccc aatctctgcg cgcgctgctg cgacgccatg ccgcccggcg gcgccgaggt
     1081 cgatatcgcc gttttgtttg ctgatgtgcg cgggtcgact gcgatggccg agaagctcgg
     1141 cccgagcgca ttcgcggcgc ggatgaaccg gttctacctg accgcaaccg aggtcctcat
     1201 ccgccacgac gccatcatcg acaagctcgt gggcgacgag gtgatggccc tgttcatccc
     1261 gggcatttgc ggtccctcct atcgtcggcg cgccgcggaa gcggcgtttg cgctcttgcg
     1321 cacggttgga gagcaactct cgcccgcgtc gcatgtgccg atcggcgtcg ccgtgaactc
     1381 cgggcttgcc tatgtcggca acgtcggaag ccaggacgtc gtcgatttca ccgcactcgg
     1441 cgataccgtc aacaccgcgg caagactggc ttcgagcgcc gccgccggcg aggtcctgct
     1501 gagcgagacc atccatgccg cgcttggcga aacttacgcc gatctggaca agcgcacact
     1561 cgcgctgcgc ggcaaggcag cgcccgtcac cgtgcgcgcg gcgcggccgt gcccgccccc
     1621 tgcttcatcc gcctgaaccg cacgctcctg ccgtccggca tgcgggttgc gacatagccc
     1681 gcggcaagac aggcctcgcg ctgcggcatc cgctcctgcg ggctgcgcag actgtcccac
     1741 caggacgcgc gctgcgcggc gcgcggcaac gaaccaccga tgatctcctc gatctcttcg
     1801 aagctcagca cgagttcggg acgcgtttgt gtcttcaggt agtcgcgcag ggggtcgtaa
     1861 tcgttcacgg ccgttccgtc gttggtgcgt acataaaccg gacgcgcggc ttttggcgtc
     1921 aacggcaacc aaccgttaac tgattaacgc atcgttgtcc tcacttaagc cgccagcaat
     1981 gtttctggcg catccagaga gatcgggagc gaaacggatg cgatttgggg ggcgcaagga
     2041 cacggccaaa cagctttggc ggcaatcagc aaagcttttg atcgcctgct ccgtcgcgac
     2101 cgtaatcggc ttctccgcaa tctgcgccag cgtcatgctc gacatgcggc acggcgagga
     2161 agagctggcg cgccagtcgc tggaaaacct cgcgatcggc atcgaggccg acatcagccg
     2221 caacatcgag ctctacgatc tctcgctgcg aaacgtggcc agcaacatcg tggcgcccga
     2281 tatcaaaaac gtctccaggc cggtgctgca cctgatcctg ttcgatcacg ccgcgaccgc
     2341 aaaccatttc ggcgctatcc aggtgtttga tgcggaaggg cgactgacgg tcgacgcctc
     2401 gacgctggat ccattgccgg aaaatcgcgc cggcgaggac tatttcacgg ttcaccgcga
     2461 ccagcccgat gtgggcctct acatcaaccg cccgatgctt catcgcggct cctacgccat
     2521 cgtgttgagc cggcgcatct ctggtcccga cggccgcttt ctcggcgtcg tcgcgggctc
     2581 gattcgcttc agctatttcc acgaattgtt cggccgtctc aatctgagcc ccggcgacat
     2641 catcaccgtg ctgcgtcgcg atcgcaccat catcatgcgc accccgttcg acctcgacgt
     2701 gatcggcaag caggtaaggg agcggcgtga cggcattgtc ctgacggcac agccgagcgg
     2761 atggttttcc gacgtcagcc ccgtggattc cacctatcgc ctgtacgtct ggagcggcgg
     2821 cctgaagccg ctggtcgtgg tggtcggcaa gccctgggac agcatcctgc ggctctggcg
     2881 catcgagttg atccggatcg gctccatcat gctgacgctg atcatgttcg tgctcgccgc
     2941 gacgctgttt cttgcgcgcg aaatcgaccg ccgtgcgctt gcggaggaca agctcgagga
     3001 gctggccacg acggatgcgc tgaccggcct caagaaccgc cgcaagttcg acgccacgat
     3061 cgagagcgaa tggcggcgcg caatgcgtta caggacgcag ctctctttgc tcatgatcga
     3121 tgccgaccat ttcaaggcct tcaacgacac gtacggtcat ccggccggcg accaggtgct
     3181 ggtcgggatc gcgatctgca tttccgactc ggtgaaacgg gccggcgact gcgccgcgcg
     3241 ctatggcggc gaggaatttg ccgtgctcct gccggggcag tcggaaaggg aggcgctcgg
     3301 cattgccgag accatccggg cgaaggttca ggagtggtcg gatgatcccg ccatcacgac
     3361 cgtcagtatc gggatcgcaa gcgtcacccc gacggccgcg ctcgactggc cgcagctcgt
     3421 gcatgccgcc gaccaggcgc tctatgcggc aaaggctgca gggcgcaact gctgcgcgct
     3481 cgccaagttg cccgacctgc tgctcgcggc ttgacacctt cgttgcggac ggcgcgcaca
     3541 gcgcgatccg gaatgacgac aaggacgcaa gcgaccttgc cgttacttcc ccgaatatct
     3601 cttcatatct gcgcgcaggc cgtcgcgcag atccgagcgc gccatgccga aggcgatgtt
     3661 ggcgcgcaga aaaccggcct tcgagccgca atcatgccgt tcgcctgcaa actccacacc
     3721 atagaatttc tgcttcttcg ccaacccgat catggcatcg gtgagctgga tttcgtcgcc
     3781 tgcgccgcgc tcatgtttgg acaggatcgc gaaaatctcc ggctgcagaa tgtatcgccc
     3841 ggtgatggag aaattggacg gcgccttgcc tttcgccggc ttctccacca tgccgtcgac
     3901 ctcgaacatg ttggctttgg cgtgacgctt gccgacgccg cagatgccgt attggtgcgt
     3961 ctgatccgcg ggcacttcct gcaccgcgag caggttcgat ttctccggca gatgtttcgc
     4021 cgccgcgacc atctgcgcaa ggcaacccgg cgtgtgcagc accagttcgt ccggcaaaac
     4081 gaccgcgaac ggctcgtcgc ccacgatgtc gcgtgcgcac cacacggcat gtcccaggcc
     4141 gagcggcgcc tgctggcggg taaagctcat ggcgcccgct tccggctggt cgcgtgccaa
     4201 aatctgctgc tcggtctttt tgccgcgcgc cgccagcgtg gcgtcgagct cgaacatccg
     4261 gtcgaaatgg tcctcgatca cacccttgtt gcggccggtg acgaagatga aatgctcgat
     4321 gccggcttcc ttggcctcat cgacgacata ttggatcaag ggcttgtcga cgatggtcag
     4381 catttccttc ggcatcgcct tggtggcagg caggacacgg gtgccgagac cggcgacggg
     4441 aaagacagct ttgcggattt tcatgggatt tgccgggcat aattttttgg ggaaggcgga
     4501 aaacggccgc cttggtagcc ggttttgaac cgggaacaaa ggcggaggga aaacttcact
     4561 gttgttaagc gattggaaac cgtaaacgag cctcctgaaa gccttaaatt tagggcagcg
     4621 ggtggaacat gaggcaaccg ggcagaccga aagcgttgac agccgtcgcg atggtagcgg
     4681 ccggcctgct tatggccggc gaagcgcggg cccagtcctc cggcggcggg ctttcaacct
     4741 tgttcagcaa catcttttcc ggtccgaaat ccgataatca ggcgccgccg cagggtgcgc
     4801 ccgccgcgac cggatcgatc ccctggagcg gcgaggacgg tgcctccggc catcccctga
     4861 tgacggcggc cgcgataagg caggcggctg cgaatttcgg caactgcgtc ggcgcgatgt
     4921 ggcccgatgc cgcgcgacgc aacgtctcgc gtgagaactt cgagcgcttc acggccgggc
     4981 tcacgcccga cctgcgcatc atggacctga tggacgcgca gccggagttc accaaatcgg
     5041 tctgggatta cctcgacatc ctggtcaacg acaaccgcct ggccaagggc aaggagatac
     5101 tcgccaaata caaggcgcag ttcgacgcaa ccgagaaggc ctatggcgtc gaccgctata
     5161 tcatcgccgc gatctggggg atcgaatcca actattcgac ccagatcggc gatcgcagcg
     5221 tgctgcaatc gaccgcgacg ctggcctgca ttggccgccg gcaaggctat ttcaaggagg
     5281 agttcctctc cgcactcgaa atcctcaatc gcggcgatct ccgcccggag cagatgcacg
     5341 gctcctgggc cggcgccttc ggcccgaccc agttcatgcc gaccgcattc aagcgttatg
     5401 ccgtcgatgc cgatggcgac ggccgccgcg atgtggtcga cgatgcgccc gacctgatcg
     5461 catcgaccgc gaacaacctg aagaaggacg gctggcagag cggccagtcc tggggctacg
     5521 aggtcacggt cccgcagggt ttcaattaca tgctggccga ccgcgcgaag gtgatgacag
     5581 cagcgcaatg ggaacatctc ggcctgaagc gcgcgaacgg ccaaccgttc ccgcaccccg
     5641 ccgacaaggg ctatctgctg gcaccggccg gcgccgaagg gccgggcttc ctgatgctgc
     5701 cgaacttccg cgtgatcatg aaatacaacc cggccgaggc ctatgcgctc gcgatcggcc
     5761 atttcgcgga ccgcttgcgc ggctcccaac ccttcgtgca gccctggccg cgacaggagc
     5821 gtgaattgtc gcgcgccgaa cggctggaac tgcaacagct tctggtccag cgcggattct
     5881 accgcggcac cccggacggc cagttcggcg gcgaaacgcg acaggcgctg cgcaatttcc
     5941 aggcctcgat cggggcgcca gccgacggat ttgcctcggc acagatgctg gaccggctgc
     6001 gcggcaactg aagcgcggcc atttgcgctg taaacggccc cggaaaccac atttcctcaa
     6061 gctgcttgac gggccgggga tagcccggtt tttctgcccg cttgcggccc gattccgcta
     6121 ttatcgactg ccgccaacca tttgcgaccg caccggatgt tcaaacgagg ccttcttctc
     6181 aaggtgttga ccgagcccgg tccctcgatc gtgctcttgg tcgtcattgc ccttctggtc
     6241 ggcatcgtgg ggcccgcgtc tgcggagttc tttaatttcg gcaatctcgg gggcggatgg
     6301 ggtcggcccg catcgcgcag cggcgggggc tggtttggca gcgatttctt cacccccttc
     6361 cagcagcagg cgccgaagcg cgtctaccag gacttttcga aggccccgcc gccggaaaag
     6421 cgcgaagcag ttccggagcg caacgtcatg gtgatgggcg acggcatggc ggactggctg
     6481 gcttccggcc tggaggatgc ctacagcgaa cagcccgata tgggcgtgat ccggaaagca
     6541 aaaagcagtt ccggcctgat caaatatcag cccaagggcg atccggccga ctggacggcg
     6601 gccgccaaat ccattctggc cgccgagaaa gccgatatca tcgtcgtaat gctcgggctg
     6661 aatgaccgcg tggcaatgcg cgagcctgcc ggcgagaaga ccgagaagcc tgccgagaag
     6721 aagagcgaca aaaaggacgc ccgcgccaag ggcgatgcga agggtgacgc caaggccgcg
     6781 gaacccaagt cggacaaggc cgaaaccgcg gtgaaaccgg aagagaaggc ggtcgatacc
     6841 gaactgccgc cggacgatgc cgacaacgcc gaagcgccgc ccgctatcgt gcccgggaag
     6901 agcacgcgtg cgccgaacgg cctctacgag tttcgcgagg atcgctgggt cgaactctac
     6961 tcgaagaaga tcgaggtgat gatcgcggtg ctgaagtcca agggcgtacc ggtggtctgg
     7021 gtcggccttc ccgcgatccg cgggcccaag ggttcccagg acatgctgtt tttggactcg
     7081 ctctatcgcg aagccgccgc gaaggccggc atcacctatg tcgacgtctg ggacggcttt
     7141 gtcgatgaac gcgggctgtt cctgcaaaag ggtccggact tcgagggcca gatccgccag
     7201 ctccgctcct atgacggcgt gtatttcacc aaacccggcg cgcgcaagct cgcgcactat
     7261 gtcgagcgcg agatcagccg cctgattgct gcgcgctcgg cgccgatcac gctgccgacc
     7321 gagccggcga cgccggatgc caatgccgtt cccggtcagc ccgcgcctcg gccgctcgca
     7381 ggaccggtcc tgccgctggt cgcctccaat gtcggcaccg atcaactgct cggcggtccg
     7441 ggaacgcggc cggccgccgt cgacgcactt gccgcgcgca ccctcgtcaa gggagagccg
     7501 ctcacgccac cgcccgggcg cgctgacgat ttcaccttgc cgcgacgcga ggtcgggtac
     7561 gagcctgcca aaggcgacac accggttgcc tccacgacac ccggcagcgg cgcaacgccc
     7621 ggaggcggcg cgacgccggc aacgccgggc gtcgcagccc tgccgcaaca gaggcagcag
     7681 cctcctttgc agcagaggag gcccttgcag cagaaccccg cgcagcagaa cccattcggt
     7741 aatttcttcg gcttcggcaa ttcatcgccg cgccagtggt cgccgcagcc gcgcccgttc
     7801 acgccgggaa tattgcggcc gccggcaccg atcggccgct cggccgcagc gtcaagcaat
     7861 ccgacgcgat aggctacagg atcagcccgc catcgaccac gatggtttgg ccgatgatgt
     7921 aggatgacaa aggcgacgcc agaaacagcg cggcgcccgc catgtcgctt ggcgtcccca
     7981 accgcctcag cgg
//
LOCUS       DATLJT010001361         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2410672,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001361 DATLJT010000000
VERSION     DATLJT010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410672"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VM910_31210"
     CDS             <1..789
                     /locus_tag="VM910_31210"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVI67011.1"
                     /translation="GERVSQPDKLDAGIYGHQIRTTPGNQTAILVARGNNAAGGKPED
                     PGALKLFGFKRGALTNLASIAPGTGLGFGPRHLDFHPSQPWVYVSIERQNKLYTYKLQ
                     SDGALGHDPIFVKDTLADPANVKPAQGAGPIHVHPSGRFVYLTNRNQGEVEFEGKKVF
                     NGGENNVAVFAIDGQTGEPSLVQTVEGHGIHLRTFGIDPSGQLLVAASIRPLAVRDGN
                     AIKTLTAGIMVYRIAGDGRLSFVRKYDVDTGKGQQFWSGMVTLT"
     gene            complement(925..1012)
                     /locus_tag="VM910_31215"
     tRNA            complement(925..1012)
                     /locus_tag="VM910_31215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(977..979),aa:Leu,seq:taa)
     gene            complement(1138..1587)
                     /locus_tag="VM910_31220"
     CDS             complement(1138..1587)
                     /locus_tag="VM910_31220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67012.1"
                     /translation="MDAVTPKSQKSAEQHSHVVRPAQMEWQKTRFPGCEVKTLLFDRE
                     TGLVTALMRFAPGAVLPDHEHVKIEQTYVLEGKLVDKEGPAAGLEVQAGEFVWRETGS
                     RHVAWTPEGGLMLAMFQIPNKFYERDGRVTDVAGQDWETVWGRALKQ"
     gene            complement(1825..>2170)
                     /gene="gpt"
                     /locus_tag="VM910_31225"
     CDS             complement(1825..>2170)
                     /gene="gpt"
                     /locus_tag="VM910_31225"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HVI67013.1"
                     /translation="ELGIRLIETVCVSSYNHITQGELNVLKDVARNIVGIGGGRGKGV
                     LIVDDLVDTGRTANVVRELLPEAHFATVYAKPMGRPMVDTFITEVSQDTWIYFPWDTG
                     LAFQPPIRDGGL"
BASE COUNT          396 a          708 c          685 g          381 t
ORIGIN      
        1 ggcgagcggg tgagccagcc ggacaagctc gatgccggca tctacgggca tcagatccgc
       61 acgacgccgg gaaaccaaac cgcgatcctg gtcgcgcgcg gcaacaacgc cgccggtggc
      121 aagccggagg atcccggcgc gctcaagctc ttcggattca agcgcggtgc gctgacgaat
      181 ctggcttcga tcgcgcctgg aaccgggctc ggcttcgggc cgcggcatct cgatttccat
      241 ccctcgcagc cctgggtcta cgtttcgatc gagcggcaaa acaagctcta cacctacaag
      301 ctgcaatcgg acggtgccct cggccacgac cccatcttcg tcaaggacac cttggccgat
      361 cccgccaacg tcaaaccggc gcaaggcgcg ggcccgatcc acgtgcatcc gagcggccgc
      421 ttcgtttatc tcaccaaccg caaccagggc gaggtcgagt tcgaaggcaa gaaggtcttc
      481 aacggcggcg agaacaacgt tgccgtgttc gccatcgatg ggcagaccgg ggagccgagc
      541 cttgtccaga ccgtcgaagg ccacggcatc catctgcgaa ccttcggcat cgacccgagc
      601 gggcagctgc tcgtggcggc gagcatcagg ccgctcgcgg tgcgcgacgg caacgcgatc
      661 aagacgttga ccgccggcat catggtctat cgcatcgccg gcgacggcag gctctcgttc
      721 gtgcgcaagt acgacgtcga caccggcaag ggccagcaat tctggagcgg catggtcacg
      781 ctgacatagc cgaacacgag gcggggcggc gcccgcggtc gtgcggcgga tttttggaat
      841 tcggaacgga attggtgccc acggccgacg gttcgctggt cgcgctgttg ggaacctcgc
      901 ccagccgacc tgaagcacgc ggggtggtgg ccgcggccgg attcgaaccg gcactccggt
      961 ttcccagaag cagattttaa gtctgctgtg tctaccgttc caccacgcgg cccaggcgca
     1021 agggcttcta gcacggcagg cgcacgcacg tcgggtttac ccgacgtcgc tctttaagag
     1081 cccgctttgg gggcaggacg ggaaatcggt tgtgccggag gcggccgggg atgtcgctta
     1141 ctgcttgagc gcgcggcccc agacggtttc ccagtcctgc ccggcgacgt cggtcacgcg
     1201 gccgtcgcgc tcgtagaact tattgggaat ctggaacatc gccagcatca gcccaccctc
     1261 gggcgtccaa gcgacgtggc ggctgccggt ctcgcgccag acgaattcgc cggcctggac
     1321 ctcgaggcca gcagcgggcc cctccttgtc cacgagcttg ccctcgagca cgtaggtctg
     1381 ctcgatcttc acatgctcgt ggtcgggaag aacagctccc ggcgcaaaac gcatcagcgc
     1441 ggtgacgagg ccggtctccc ggtcgaacaa cagcgtcttg acctcacagc cggggaagcg
     1501 ggtcttctgc cattccatct gggcgggccg caccacgtgg gagtgctgct cggcggattt
     1561 ttgcgacttc ggcgtgacgg cgtccatggc cactcctcct cgggccgcga tgtcgcggcg
     1621 ttgtgcgcgc aatcatagcc gaacgcgggg ctagtgtccc gattccgaag ttcgcaaacg
     1681 ctcgcggcac cctctgatgc gaacttcgga atcgaaggga cactagcaac tttatgactc
     1741 cagggcggtt ttggatttga agttcctaaa cgagctccta gccacaatga taggaacttc
     1801 aaatccaccg cccctagcgc gcctctacaa tccaccgtcc cggatcggcg gctggaaggc
     1861 aaggccggtg tcccagggaa aatagatcca ggtgtcttgc gatacttcgg taatgaacgt
     1921 atcgaccatg ggtcggccca tgggcttggc gtagacggtc gcgaaatgcg cctcgggcag
     1981 caactcgcgc accacgtttg ccgttctgcc ggtatcgacg agatcatcga cgatcaacac
     2041 gcccttgccg cgcccgccgc cgatgccgac gatgttgcgt gccacgtcct tgagcacgtt
     2101 gagctcgccc tgcgtgatgt ggttgtagct cgagacgcac accgtctcga tcagccgaat
     2161 gccgagctcg
//
LOCUS       DATLJT010001362         3188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2870881,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001362 DATLJT010000000
VERSION     DATLJT010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870881"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM910_31230"
     CDS             complement(<1..200)
                     /locus_tag="VM910_31230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI67014.1"
                     /translation="MNEVVKLERHDVIGIVTVNNPPVNALSAAVRGGILDCMKKAIAD
                     PEVKAIVLTCAGRTFIAGADIT"
     gene            382..1254
                     /locus_tag="VM910_31235"
     CDS             382..1254
                     /locus_tag="VM910_31235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVI67015.1"
                     /translation="MKRSGKKVATDRSFVVALSRGLDVLRAFQPNDGLLGNQEIAART
                     NLPKPTVSRLTYTLTKLGYLTPVPRFEKYQLAPSAMALGYAALANLGLRHLSEPFREE
                     VMRATGGAVAVGGRDRQSMIYFGQSRNGLTLGVQLDVGSRIPIATTAMGRAYIWALPP
                     DERSALLRELREHYGSRWPKIRDGIERSGEIVAKRGFAVSAGEWQSDVAAVGVALKLN
                     DGTGPYAFNCGAPAFRFTEDRLLNDIGPRLVAMVRNIEAALGGVAPQPTQSKKESKKL
                     KSGGKVARVAEGIR"
     gene            1315..2112
                     /locus_tag="VM910_31240"
     CDS             1315..2112
                     /locus_tag="VM910_31240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67016.1"
                     /translation="MTQTALAPGDDPLLAVRDVSVVFGGIVALNGVSFDMRQGQILGL
                     IGPNGAGKTTLFNCLSRLYQPSAGDILMEGVSILSRPPHRIAEIGIGRTFQNVALFPN
                     LSVLDNVRVGTHSRTRSDIVSDSLKLAWVRRSETAVNKKVHEILKYLDLDDVAHTVVS
                     GLPFGTQKRVELARALAADPKILLLDEPAGGLNHEEVHVLGDLIRKIRDDRKITVLLV
                     EHHMGLVMSIADHVVALNFGRKLAEGTPAQVQSDPDVIKAYLGSKDQ"
     gene            2109..2822
                     /locus_tag="VM910_31245"
     CDS             2109..2822
                     /locus_tag="VM910_31245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67017.1"
                     /translation="MTTMLNVKDLRAYYGHVQALHGLSFSLNEGSLTTLLGANGAGKT
                     TTLRAICNMVRSTGAIEFEGQPLSGRSTENIVRLGIAHVPQGRGTFTTMTVEENLQLG
                     AISRKDKSGIVADIERMYEHFPVLKQRYTQQAGTLSGGEQQMLAVARALMLRPRLMLL
                     DEPSFGLAPLIVRDLFKILGKINREDKVTILVVEQNAQLALELADQAYVIETGRIVMS
                     GGAKEIANNEDVRKSYLGY"
     gene            2979..>3188
                     /locus_tag="VM910_31250"
     CDS             2979..>3188
                     /locus_tag="VM910_31250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI67018.1"
                     /translation="MELFTNQILAGISTGAIYACMALAVVMIYQAIDHLNFAQGEMAM
                     FSTFVSWQLMQWGIPYWWAFLLTLAF"
BASE COUNT          590 a          997 c          995 g          606 t
ORIGIN      
        1 tcggtgatgt cggcgccggc gatgaaggtg cgccccgcgc aggtgagcac gatggccttc
       61 acctcgggat cggcgattgc ctttttcatg cagtccagaa tgccgccgcg cacggcggcg
      121 ctcagcgcgt tgacgggagg attgttgacg gtgacgatgc cgatgacgtc gtgacgttcg
      181 agcttgacca cttcgttcac gctacgctcc ttggagttgt tcttggtttc ttgacttgcg
      241 ttgagtagct gcccggcctt gcgatgagga gctacgtgtc aatctctggg tttcgcactg
      301 cggaatttaa ttccacatct tgacagcgag ggttattttg aagcaggtcc gttgtcaacg
      361 aggtcctgac aacggcggtc catgaaacgc tcgggcaaga aagttgcgac cgatcgcagc
      421 ttcgtcgtcg cgctctcgcg cggtctcgat gtgctgcgcg cgttccaacc caacgacgga
      481 cttctgggca atcaggagat cgcggcgcga accaatttgc caaagccgac cgtctcacgg
      541 ctaacctaca ccttgaccaa gctcggttat cttacgccgg ttccgcgctt tgagaagtat
      601 caactggccc cttcggcgat ggcgctcggc tatgcagcgc tggcgaacct cggactgcgt
      661 catctgtccg agccgttccg cgaggaggtg atgcgcgcca ccggcggcgc ggtcgcggtc
      721 ggcgggcgcg accgccaaag catgatctat ttcggccaga gccggaacgg cctgacgctg
      781 ggggttcaac tggacgtggg ttcgcgaata ccgatcgcaa ccaccgccat gggacgcgcc
      841 tatatctggg cgctgccgcc ggatgaacgg tccgctctgt tgcgcgaact gcgcgagcat
      901 tacggcagcc gctggcccaa aatacgtgac ggcatcgaac ggtccggcga aatcgtcgcc
      961 aagcgcggct ttgcggtgtc ggccggcgag tggcagagcg acgtcgcggc ggtcggcgtt
     1021 gcactaaagt taaatgacgg gacagggcct tacgcgttca attgcggtgc gccggcattc
     1081 cgctttacgg aagatcggct gctcaacgac attggacctc gcctcgtggc gatggtaagg
     1141 aacatcgaag cggcccttgg gggcgtggcg ccgcagccga cacaatcaaa aaaagaaagc
     1201 aagaaattga aatcaggagg gaaagttgca cgtgtcgccg aggggatcag ataggctcta
     1261 tcgtcaggac cggcgaaaga tgccgggtcg gtccggccgc gcgggccgga ccacatgacg
     1321 caaacagcgc ttgcgccggg ggatgacccc ctgctcgcgg ttcgcgacgt cagcgtcgtg
     1381 ttcggcggca tcgtcgcgtt gaacggcgtg tccttcgaca tgcgtcaggg ccagatcctc
     1441 ggcctgatcg gtccgaacgg cgccggcaag accacgctgt tcaattgcct ctcccgtctc
     1501 tatcagccga gcgccggcga catcctgatg gagggcgtga gcatcctgtc gcggccgccg
     1561 caccgcatcg ccgagatcgg catcggccgc acgttccaga acgtcgcgct gttccccaat
     1621 ctttcggtgc tcgacaatgt tcgcgtcgga acccattctc gcacccggag cgacatcgtc
     1681 agcgactccc tgaagctcgc ctgggtgcgc cgcagcgaga ccgcggtcaa caagaaggtc
     1741 catgagatcc tgaaatatct cgatctcgac gacgtcgcgc acacggtcgt gtccgggcta
     1801 ccgttcggaa cccagaagcg cgtcgagctg gcgcgtgcgc ttgcggccga tccgaagatc
     1861 ctgttgctcg acgaacccgc cggcggcctc aaccatgaag aggtccatgt gctcggcgac
     1921 ctcattcgca agatccgcga cgaccgcaag atcacggttc tcctggtcga gcatcacatg
     1981 ggtctcgtga tgtcgatcgc ggaccacgtg gtcgccttga atttcggtcg caagcttgca
     2041 gaaggcacgc cggcgcaggt gcagtccgat cccgacgtca tcaaggccta tctcgggagc
     2101 aaggaccaat gacgacgatg ctcaacgtca aggatctgcg cgcctattac ggccacgtgc
     2161 aggcgctgca cgggctgagc ttttccctga acgagggatc gttgaccacg ctgttggggg
     2221 ccaacggcgc cggcaagacc acgacgcttc gcgcgatctg caacatggta cgctcgaccg
     2281 gcgccatcga gttcgagggc cagccgctct ccggccgctc gaccgagaac atcgtccggc
     2341 tcggcattgc ccatgtgccg cagggccgcg gcaccttcac gacgatgacg gtcgaggaga
     2401 acctgcagct cggggcgatc agccgcaagg acaagtccgg catcgtcgcc gatatcgagc
     2461 ggatgtacga gcatttcccg gtgctgaagc agcgctacac gcaacaggcc gggacgctgt
     2521 cgggcggcga gcagcagatg ctcgcggttg cccgtgcgct gatgctgcgt ccgcgcctga
     2581 tgctgctcga cgagccgtcg ttcgggcttg cgccgctcat cgtacgcgac ctcttcaaga
     2641 tcctcggcaa gatcaaccgg gaggacaagg tgaccattct ggtggtcgag cagaatgccc
     2701 agcttgcgct cgaactcgcc gaccaggcct atgtgatcga gaccggccgc atcgtgatgt
     2761 cgggcggcgc caaggagatc gccaacaacg aagacgtccg caaatcgtat ctcgggtatt
     2821 gaggtgagat gatgccgtcg atcaccgtgc accccaccac gtctcgcatc ccgcttcgcg
     2881 cgatgctcgc cgaccctctc cctccagggg agggtggtgg cacctccgcg ttctcaacgg
     2941 ttcaatgcga actcatcgcg ccttagggag ccgacggcat ggaacttttc accaatcaaa
     3001 tcctggccgg catctccacc ggggcgatct atgcctgcat ggcgcttgcg gtggtcatga
     3061 tctaccaggc gatcgaccat ctcaatttcg cgcagggcga aatggcgatg ttctcgacct
     3121 tcgtctcctg gcaattgatg caatggggca tcccctactg gtgggctttc cttctcaccc
     3181 tcgccttc
//
LOCUS       DATLJT010001363         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3790701,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001363 DATLJT010000000
VERSION     DATLJT010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3790701"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VM910_31255"
     CDS             complement(<1..1164)
                     /locus_tag="VM910_31255"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVI67019.1"
                     /translation="MRNFHLAGRSTVHALNAMVATSHPLAALTAIDVLREGGTAADAA
                     VAASALLAVIEPQSTGIGGDCFALIQPKGEGKITAYNGSGRAPAAARVEWYLERNIHA
                     VPLTSAHAVSIPGAVDAWQVILRDHGKFGLDRLLQPAIKAAEEGYVVAPRIAFDWKNQ
                     FEKLRKGTNTERYLLPHGKPAVAGDVIRQPELGKTLRAIAKDGRDAFYKGPIAQDMVE
                     TLRSIGGLHTTDDFAAHTTEVTSPIGTDYKGLEAWQCPPNGPGITMLVMLNILSHFDL
                     TKFAPLSVERFHLEAEAARVAYMMREQYIGDPAQVDVDVPRILAKNFAADYVGKIRMD
                     ALLDLPNVAPPMNPSTVYITVVDRDRNVCSFINSVAHAFGAAIVSNKTGILLQN"
     gene            1323..2063
                     /locus_tag="VM910_31260"
     CDS             1323..2063
                     /locus_tag="VM910_31260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HVI67020.1"
                     /translation="MPYKVAAFYQFAGLPDFRALREPLRAICARLSLKGSVLLAHEGI
                     NGMLAGDEAGIAGLVAELRHGDLFGGRLNNLELKFSSAEAIPFGRLKVRLKKEIVTLG
                     DGTADPTRQVGIYVAPRDWNALISSPDTLLLDTRNAFEVSMGTFAGACDPQLASFSQF
                     KDFAAKHLDPLEHRRIAMFCTGGIRCEKASSHLLARGFAEVYHLKGGILKYLEEVPET
                     ESRWRGECFVFDERTALGHGLRERGARP"
     gene            2060..2272
                     /locus_tag="VM910_31265"
     CDS             2060..2272
                     /locus_tag="VM910_31265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HVI67021.1"
                     /translation="MSEQQLSNRIDTLEARLMFQDVTIETLNATVTAQWQEIDLLKRQ
                     IADLNERLREAEADTPAPANERPPHY"
     gene            complement(2376..2627)
                     /locus_tag="VM910_31270"
     CDS             complement(2376..2627)
                     /locus_tag="VM910_31270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67022.1"
                     /translation="MAEKFNPAPHDKHAADPREAARADAEMHARLEAGLIDTFPASDP
                     VSAAQPDPTKHDRRRKAPAARDSAPQTPSLWEKVKAAFR"
     gene            2820..>3732
                     /gene="hemE"
                     /locus_tag="VM910_31275"
     CDS             2820..>3732
                     /gene="hemE"
                     /locus_tag="VM910_31275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVI67023.1"
                     /translation="MMRQAGRYLPEYRDVRARAGGFLDLCFTPELAAEVTLQPIRRFG
                     FDAAIIFSDILVIPYALGRAVRFEAGEGPRLDPLDTPKKIKTLASRADFAKLEPVFEA
                     LRRVRSALAPEVALIGFCGAPWTVATYMVAGQATPDQAPARIMAYSHPDAFAKIIDAL
                     VENSIEYLLGQLEAGADALQIFDTWAGVLSPREFARWSIEPTRRIVAGVRAKAPGAKI
                     IGFPRGAGAHLPDYVEATGVDAVSIDWAAEPRLIRERVQAKVAVQGNLDPLVLIAGGA
                     ALDAAIDDVLSNYAQGRLVFNLGHGILP"
BASE COUNT          666 a         1150 c         1214 g          702 t
ORIGIN      
        1 gttctgcagg aggatgccgg tcttgttgga gacgatcgcc gcgccaaagg cgtgcgcgac
       61 cgagttgatg aaggagcaga cgttgcgatc cctgtcgacg acggtgatgt agacggtcga
      121 cggattcatc ggcggcgcca cattgggcag atcgaggagt gcgtccatcc ggattttgcc
      181 gacatagtcg gcggcaaaat tcttcgccaa aatcctggga acgtcgacgt cgacctgagc
      241 gggatcgccg atatattgct cgcgcatcat gtaggcgacg cgcgcggctt ccgcctcgag
      301 atggaagcgc tcgacgctca aaggcgcaaa cttcgtcaga tcgaaatgcg agaggatgtt
      361 gagcatgacc agcatggtga tgccgggccc gttcggcggg cactgccatg cctccagtcc
      421 cttgtagtcg gtgccgatcg gcgaggtgac ctcggtggtg tgggcggcga aatcgtccgt
      481 cgtatgcagg ccgccgatcg atcgcagcgt ctcgaccatg tcttgcgcga tcggtccctt
      541 gtagaaggca tcccggccgt ccttcgcgat cgcgcgcaag gtcttgccaa gctccggctg
      601 gcggatgaca tcgccggcca ccgcaggctt gccgtgcggc aacagatagc gctcggtgtt
      661 ggtgcctttc ctcaacttct cgaactggtt cttccagtcg aaggcgatgc gaggggcgac
      721 gacatacccc tcctcggcgg ccttgattgc cggctgcagc aaacggtcga ggccgaactt
      781 gccgtggtcg cgcaggatca cctgccaggc gtcgaccgcg ccgggaatcg aaaccgcgtg
      841 cgcggaggtc agcggcaccg catggatgtt gcgctcgaga taccattcga ccctggccgc
      901 cgccggcgcg cggccggaac cgttataggc cgtgatcttg ccctctccct tcggctggat
      961 cagcgcaaag caatcgccgc cgatgccggt cgattgcggc tcgatcaccg caagcagtgc
     1021 agaggccgca accgccgcgt cggcagcagt gccgccttcg cgcagcacgt cgattgcagt
     1081 gagcgcggca agcggatgcg aggtcgccac catcgcattc agggcgtgga ccgtggagcg
     1141 gccggcgaga tggaaattcc tcatgcgaaa agctcacttc ttccctggcg gaaccagttc
     1201 cgggcgggcg ggggttacat gacatattga tgagggactc ggcaatgggc tggtatacca
     1261 gcggcccgca tgtcgtcccc gtgaaagttg agtggctcgc ccgcccctga tagaccgcag
     1321 ccatgcctta caaagtcgcc gctttttatc aattcgccgg cctgccggac tttcgcgcgc
     1381 tgcgagagcc gctgcgtgcg atctgtgcac gactcagcct gaaaggcagc gtgttgctcg
     1441 cccacgaggg catcaacggc atgcttgcag gcgacgaagc cggcatcgcc ggattggttg
     1501 cggaattgcg gcatggcgac ctcttcggcg gccggctgaa caacctcgaa ctgaagttct
     1561 cttccgcaga agcaatcccg ttcgggcggc tgaaagtgcg tctgaaaaag gagatcgtca
     1621 cgctcggcga cggaacggct gatccgacgc ggcaggtcgg catctatgtc gcgccgcgcg
     1681 attggaatgc gctgatttcc tcgcccgaca cgctgctgct cgacacccgc aatgccttcg
     1741 aagtgagcat gggcacgttt gcgggcgctt gcgatccgca acttgcaagc ttcagccaat
     1801 tcaaggactt cgccgcaaaa catctcgacc cgctcgaaca ccgcaggata gcgatgttct
     1861 gtaccggcgg catccgctgc gagaaagcga gcagccattt gctcgcgcgc ggctttgccg
     1921 aggtttatca cctcaagggc ggcatcctga aatatctgga agaggtgccg gagaccgaga
     1981 gccgctggcg cggcgagtgc tttgtgttcg acgagcgcac ggcgctgggc catggcctgc
     2041 gcgagcgggg tgcgcgccca tgagcgagca gcagctcagc aatcgtatcg atacgctcga
     2101 agcgcgcttg atgtttcagg acgtgaccat cgaaacgctg aatgcgaccg tcaccgcgca
     2161 atggcaggag atcgacttgc tgaaacggca gatagcggat ttgaacgagc ggctgcgcga
     2221 ggcggaagcc gatacgccgg cgcctgcaaa cgaacggccg ccgcattatt aagacacgaa
     2281 agttctctct gcccgcaagc ggagcgaggt gaaaaacgag ttcattggac acagcaattc
     2341 ggaaggcgat tgtgccggcc accgcacgtc gtcgtttagc ggaacgccgc cttcaccttt
     2401 tcccaaagtg agggtgtctg cggcgcgctg tcccgggcag ccggtgcctt tcggcgacga
     2461 tcatgcttgg tcgggtccgg ttgcgcggca ctgacgggat ccgatgccgg aaaagtgtcg
     2521 atcagcccgg cttccaacct tgcatgcatc tccgcgtcgg cccgcgccgc ttcgcgcgga
     2581 tcggccgcat gcttgtcgtg aggcgcagga ttgaatttct cagccatgca agctttcctc
     2641 tcgaagcagg taacgctcca cggctcatgg cggttccccg attggccaag acctccgcgg
     2701 cggtgtatca ggaaccaatt ctttctcgtc aggaacccga tcgcgtgacc ccgaaaatcg
     2761 cagcaaatcc cttcctcgac gtgctgtcgg gccgaagacg ggcagtgccg ccgatctgga
     2821 tgatgcgtca ggccgggcgc tatcttcccg aatatcgcga cgtgcgcgcc cgggccggcg
     2881 gctttctcga tctttgcttc acgccggaat tggcggcgga agtgacgctg cagccgatcc
     2941 gccgcttcgg tttcgatgcc gcgatcatct tctccgacat cctggtgatc ccgtatgcgc
     3001 tcgggcgcgc ggtgcggttc gaagccggcg aaggcccgcg gctcgatcca ttggatacgc
     3061 cgaaaaagat caagacgctt gcttcgcgcg ctgactttgc caagctcgaa ccggttttcg
     3121 aggcgttgcg gcgcgtgcgt agcgctcttg cgccggaggt cgcgctgatc ggcttttgcg
     3181 gcgcgccctg gacggtcgca acctatatgg tcgcgggcca ggcaacgccg gaccaagcgc
     3241 cggcgcggat catggcgtat tcccaccccg acgcctttgc gaaaatcatc gacgcgctgg
     3301 tcgagaattc gattgagtat ctgctggggc aactcgaggc cggcgccgac gccttgcaga
     3361 tcttcgacac ctgggcgggc gtgctgtctc cgcgcgaatt tgcgcgctgg tcgatcgagc
     3421 cgacccgccg cattgtcgcg ggcgtgcgtg cgaaggcgcc gggcgcaaag atcatcggct
     3481 ttccccgcgg ggcgggcgcg catctgcccg attatgtcga ggcaaccggc gtcgatgcgg
     3541 tcagcatcga ctgggccgcg gagccgcgcc tgatccgcga gcgcgtgcag gcgaaagtgg
     3601 cggtgcaagg caatctcgat ccgctcgtgt tgatcgccgg aggtgctgcg ctcgatgccg
     3661 cgatcgacga tgtgctgtcg aactacgcgc aaggccggct ggtcttcaat ctcggacacg
     3721 gcattttgcc gg
//
LOCUS       DATLJT010001364         1572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2181566,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001364 DATLJT010000000
VERSION     DATLJT010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181566"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VM910_31280"
     CDS             complement(<1..478)
                     /locus_tag="VM910_31280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVI67024.1"
                     /translation="MIGAVPRLALAADESTAEKPDFTLRIGTGLVELSPDHIVSTTLY
                     NGQFPGPLLRFTEGQRVVVDIHNDTDTPELVHWHGQMIPSDVDGASEEGSPFVPAHGM
                     RRISFLPKPSSFRFYHTHVVAGGDLNRGTYTGQAGPVYIEPKDNPGAYDREIFLVMK"
     gene            complement(953..1477)
                     /locus_tag="VM910_31285"
     CDS             complement(953..1477)
                     /locus_tag="VM910_31285"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HVI67025.1"
                     /translation="MTQPLKALRTRAANLEDAVAIAEIYNQGIADRVATFETEPRTPA
                     QIAEWFKAGHLIMIAEADGTGAVAFAAAFPHSSRPCYAGIGEFSVYVARAHRGHRAGR
                     AVLSALVEAATGRGFHKLVSRVFPENAASRVLLNRLGFEEIGTHRRHGQLDGVWRDCV
                     IVERLLEHGTAPDL"
BASE COUNT          311 a          489 c          493 g          279 t
ORIGIN      
        1 ccttcatcac cagaaaaatt tcgcggtcat aggcgcccgg gttgtccttt ggttcgatat
       61 agaccggccc agcctggccg gtgtaagtgc cgcggttcag gtcgcctccg gccacgacat
      121 gggtgtggta aaaccggaag ctcgagggct tcggcaagaa cgagatccgg cgcattccgt
      181 gcgccggcac gaacggcgag ccttcctcgg aggcgccgtc gacatcgctg gggatcatct
      241 gcccgtgcca gtgcaccagc tcaggcgtgt cggtgtcgtt gtggatatcg acgacgaccc
      301 gctgcccttc cgtgaaccgc agcagcggcc cggggaactg gccgttatag agggtggtcg
      361 agacgatatg gtccggcgac agctcgacca gtcctgtgcc gatgcgcaac gtgaagtccg
      421 gcttttccgc ggtgctttcg tcggcggcca acgcgaggcg cggcaccgca ccgatcaacg
      481 gaaccagtcc ggcatatgtg agaaaagagc gccgatcgat cagcgcaacc tcactccttc
      541 cgccgccacg attcacagga tgagcgcgag ccaaaggaga cgctgcttct ccctgagctc
      601 agggagaatc cgcgaaaaaa ttcgaaaatg attggtctca acggtaatgc tctcatccaa
      661 tcaataggtt atgagagcgc cctgcgcatc gagcagggag gcgagcaggg agccgaattg
      721 cggctctgag ggagcgagca gggagaggga tggggcactc ccttccggta ggttcctccc
      781 tgtaacgata gagagcatac gttttgatct tgatgacaag atggcgatgc agatgccggt
      841 tggtggtgcg cccggctgtc atccaagcga ccgtgagcca ctcaaggcaa aacgcctctc
      901 cgaccagcgc tacccgtcca actcgtgatg aaccagcgcc ggcaatcttc gctcacaagt
      961 ccggcgcggt gccgtgctcg agtagacgct cgacaatcac gcaatcgcgc cagaccccat
     1021 ccaattggcc gtgcctgcga tgtgtcccga tctcctcaaa cccgagccta ttcaggagca
     1081 cccggcttgc tgcgttttcc ggaaatactc ggcttacgag cttgtggaaa cccctgccgg
     1141 tggcggcctc gaccagggcg gagagcaccg cccggccggc tcgatgcccg cgatgtgccc
     1201 gcgcgacgta taccgagaac tcgccgattc cggcatagca gggccggctg ctatggggga
     1261 acgctgcggc gaaggcgacg gctccggtcc cgtcggcttc agcgatcatg atgaggtgcc
     1321 ccgccttgaa ccactcggcg atttgcgccg gcgtccgcgg ctcggtctcg aaagtggcca
     1381 cccgatcggc gatgccctga ttatagattt cggcgatcgc gacggcgtcc tctaagttcg
     1441 ccgcacgcgt acgcagggct ttgagaggct gcgtcaatcg ctcgacccct gacgacgcaa
     1501 tagcggcagc gcgagtgcca aggcaagaag ctgtgcgaga ataaagcccg gcgcatcact
     1561 cgggcggatg cc
//
LOCUS       DATLJT010001365         4335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1952199,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001365 DATLJT010000000
VERSION     DATLJT010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952199"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1359)
                     /locus_tag="VM910_31290"
     CDS             complement(130..1359)
                     /locus_tag="VM910_31290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67026.1"
                     /translation="MDGQIIQQRSSYRQGLVLGLTMAEIMILLVFCLLIAMATFLKRE
                     ETRREAAETQLQQQQLQSKRDREIVAALRQDAALAEKIKSASGLSDPQAIDQYWRELV
                     DGRTVAQEIEKSGLSLKQLQDGLSTAANLRNNGIDVDRAIRNSRLVEAIYRAMAKPGE
                     PQVSTQAILDTIERGKTGVAANGHQWPPIITMSEAGGYYFESGSAELSPVFHYTLLNE
                     TPQKILDNIRKYDVDVIEVVGHTDERPIGFRASSSNLDRDLTAVLKNDAAVASLVPAD
                     NAGLGLARAVSVVSVLRQSPLLSGYKMIPLSGAQLVNTDETLALWGSPGDIQERRRIE
                     IRLRKSVPHEATAGLGGNLGTGLGTNPGTGLGAGAGAGIGPAPIMVPAPVTAPAHRTG
                     TPPPPWMAKPRSFSAPY"
     gene            complement(1384..2379)
                     /locus_tag="VM910_31295"
     CDS             complement(1384..2379)
                     /locus_tag="VM910_31295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67027.1"
                     /translation="MALNRPGLDLPSDLPPGLMNGLLFFAVVVVGSSYIIASKLNNFG
                     AFAVTAVPVLIMIAYAVVLGGVRLFHLRDDQSGDNLYYMGFLFTLTSLAVSLYQFSAA
                     GSAEQIVQNFGIAIASTIAGIALRILFNQMRRDPIEVEATARLELAEASRRVKRELES
                     TILEFSYFRRMTQQSVTDALDEMKEGIGAAGKPLEEASRKSGETLDTLNERMVRTLEA
                     VSEQLVQEGELLSRSSAHIVGAIDAVVTKLASLQAPEQAVENKLAPLIESLTAAVASF
                     GESAEAQARNMESNLKHTQTVVLAITQLLQEVRAAGITHPAAGAAGAWSSRHLER"
     gene            complement(2385..3221)
                     /locus_tag="VM910_31300"
     CDS             complement(2385..3221)
                     /locus_tag="VM910_31300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67028.1"
                     /translation="MARRKPRRSREPGFGWAGLGGLLLLLLAIASAGALAYFYFSAPA
                     RPVLDAQSLCPVDGPQGITVVLVDTSDDLPETTRREVLGELDDLITTLPPYHKLDIRV
                     LDIAGVKSRSLFSKCNPGDGAGLSEWTDNPRLARLRWIENFRKPAAEAVKSSVASAKA
                     ASSPIMAAIQDIAIEQFSSNASQNVRKTLYVISDMIEYTRDYSQYPRAGDLTYQRYRQ
                     SPAYLKFRTDLHGATVIIRYVTRQTNGQPLLDGVKHMEFWKAWIEDNRGTFGGAKRLQ
                     GA"
     gene            complement(3214..>4335)
                     /locus_tag="VM910_31305"
     CDS             complement(3214..>4335)
                     /locus_tag="VM910_31305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67029.1"
                     /translation="PEAVGDFRAEATVGRDELFVLRRRLNESEIERERFRARHRIERP
                     ARLASPGKIILKIGVLAILFITEVVINGSFLAKANVGGLLGGAAQAVTFAALNILASF
                     FWGMVLIRLINRRNFFLKLIGLLSFLAYLAFAIALNLTLSHLREIPPAVAVSGDVGEV
                     VLHRLLTAPYALTDINSWVFFSIGLIFSLIAMADGLMFFDPYMGYAGLERRWVEATKQ
                     FASARSELIERLRDIREDATEVMNEAARDLSVRRSEYDSLLQGRGRLAQRFSQHQNQI
                     EQAGRALLEIYREANRRARATPAPDYFAKAYAMDRIVYSGGDPDPSARENLRKMIADT
                     QELLTGQIKAIHEAFDSAVRTYREIDELLPEKKPEKKGG"
BASE COUNT          766 a         1381 c         1399 g          789 t
ORIGIN      
        1 cagcgcctgc accgccgccc gtcgccgcgc gatgtccgct ggcgggcggc ggctgccgcg
       61 tgaaaaggcg aactcgccga gaacttccgg cctccgcgag ggcgcctgga agcgctgttc
      121 ggcccgacgt cagtagggcg cgctgaagga ccgcggcttc gccatccacg gagggggcgg
      181 cgtgccggtg cggtgtgccg gcgcggtgac cggggccggg accatgatcg gcgctggccc
      241 gatgcctgca ccggcgccgg cgccaaggcc cgtgccagga tttgtgccga gaccagtgcc
      301 gagatttccg ccgagacctg cggtcgcctc gtgcggcacc gacttgcgca agcggatttc
      361 gatgcgccgc cgctcctgaa tatcgccggg cgaaccccaa agcgccagcg tttcgtcggt
      421 gttcacgagc tgcgcgccgg acagcgggat catcttgtat cccgaaagca gcgggctctg
      481 ccgcagcacg ctgaccacgg acaccgcgcg cgcgagccct agcccggcat tatcggcggg
      541 gacaagactc gcaacggcgg cgtcgttctt cagcaccgcg gtcaggtccc gatcgaggtt
      601 ggagctcgac gcacgaaagc cgatcggccg ctcgtcggtg tggccaacga cctcgatgac
      661 atcgacgtcg tatttcctga tgttgtcgag aatcttctgc ggcgtctcat tgagcagcgt
      721 atagtggaag accggcgaca actcggcgct cccgctttcg aaataatagc cgccggcctc
      781 gctcatggtg atgatgggcg gccattgatg gccgttcgcc gcgacgccgg tcttgccccg
      841 ttcgatggta tccaggatcg cctgcgtcga cacttgcggc tcgcccggct tcgccatcgc
      901 gcgatagatc gcctcgacca gccgtgaatt gcgaatggcc cggtcgacgt cgatgccgtt
      961 gttgcgcagg ttggcggcgg tcgaaagacc gtcctgaagc tgtttcagcg acagcccgct
     1021 cttctcgatc tcctgcgcca cggtcctgcc gtcgacgagc tcgcgccaat actgatcgat
     1081 cgcctgcgga tcgctcaggc cggatgcgct cttgatcttc tccgccagtg cggcgtcctg
     1141 gcgcagcgcg gcgacgatct cgcggtcgcg cttgctctgc aattgctgct gctgcaattg
     1201 cgtttccgcc gcctcgcgac gggtttcttc ccgcttcagg aaggtcgcca tggcgatcaa
     1261 aaggcaaaac accagaagga tcatgatctc ggccatggtg agaccgagca ccaggccttg
     1321 ccggtaggaa gagcgctgct gaatgatctg tccgtccatg ggaggcctcg atgaaatcag
     1381 ggactaacgc tcgagatgtc gcgaactcca ggcgcctgcg gcaccggcag ccgggtgcgt
     1441 aatcccggcg gcgcggactt cctgcaaaag ctgggtgatc gcgagcacga cggtctgcgt
     1501 gtgcttgaga ttgctttcca tattcctcgc ctgcgcctcg gcgctttcgc cgaacgaagc
     1561 aaccgcggcg gtaaggcttt cgatcagcgg cgcgagcttg ttttcgacgg cctgctcggg
     1621 cgcctggagc gaagcgagct tggtcacgac ggcgtcgatc gctcccacga tgtgcgcgga
     1681 gctgcgcgaa agcagctcgc cctcctgcac caattgctcc gacaccgcct cgagcgtccg
     1741 aaccatccgt tcgttcaggg tgtcgagcgt ttcgcccgac ttccgggacg cctcttccag
     1801 cggcttgccc gcggcaccga tgccttcctt catctcgtcc agcgcgtcgg tgaccgattg
     1861 ttgggtcatg cggcggaaat agctgaactc caggatcgtg ctctcgagct cccgcttcac
     1921 ccgccgcgat gcctcggcga gctccagcct tgcggtggct tcgacctcga tcggatcgcg
     1981 ccgcatctgg ttgaacaaga tgcgcagcgc aatgccggcg atcgtggaag cgatggcgat
     2041 gccgaaattc tggacgattt gctcggccga gcccgccgcg gagaactgat agagcgagac
     2101 ggcaaggctc gtcagcgtga acaggaagcc catgtagtac agattgtcgc cggactggtc
     2161 gtcgcgcagg tgaaacaggc gcacgccccc gagcaccacc gcataggcga tcatgatcag
     2221 aacggggact gcggtcaccg caaaggcgcc gaagttgttc aacttcgagg caatgatgta
     2281 gctgctgccg acgacgacca cggcgaagaa caacagcccg ttcatgagcc ccggcggcaa
     2341 atcggacggc aggtccagtc ctggtcggtt cagcgccacg cgagtcaagc cccctgcagc
     2401 cgcttggcgc cgccaaacgt gcctctgttg tcctcgatcc aggccttcca gaattccatg
     2461 tgtttcacac cgtcgagcaa gggctggcca ttcgtttgcc gggtcacata gcggataatg
     2521 acggtcgcac catggagatc ggtccggaac ttcaggtagg ccggcgactg cctgtagcgc
     2581 tgatacgtga ggtcgccggc acggggatat tggctgtaat cccgcgtata ctcgatcata
     2641 tccgatatca cgtagagcgt cttgcggacg ttctggcttg cattgctgga aaactgctcg
     2701 atcgcgatgt cctggatcgc agccatgatc ggggagcttg cggccttggc ggatgcgacg
     2761 ctgcttttca cggcctccgc tgccggtttt cggaaattct cgatccatcg aagccgcgcg
     2821 aggcgcggat tgtcggtcca ttcgctcagg ccggcgccat cgccgggatt gcatttggag
     2881 aacaacgaac ggcttttgac gccggcgatg tcgagcaccc ttatgtcgag cttgtgatag
     2941 ggcggcaatg tggtgatgag gtcgtcgagc tctcccagca cctcgcgccg tgtggtctcg
     3001 ggcagatcat ccgacgtgtc gaccaggacg accgtgatgc cttgcggacc atcgaccggg
     3061 cagaggctct gggcgtcgag caccggccgc gcgggagcgg agaaataaaa ataggcgagt
     3121 gcgccggcag aggcgatcgc gagcaggagc agcagcaacc cgccaagccc cgcccagccg
     3181 aagcccggct cgcgcgaacg tcgcggcttg cgtctagcca ccctttttct ccggcttctt
     3241 ctccggcagg agctcgtcga tctcgcgata ggtgcgcacc gcgctgtcga aagcctcgtg
     3301 aatggccttg atctgcccgg tcaggagctc ctgggtgtcc gcgatcatct tgcgcaggtt
     3361 ttcccttgcg ctcgggtcgg gatcgccgcc gctgtagacg atgcgatcca tcgcgtaagc
     3421 ctttgcgaaa tagtccgggg ccggcgtcgc gcgagccctc cggttcgcct cccgatagat
     3481 ttcgagcaag gcgcggccgg cctgctcgat ctggttctgg tgctggctga agcgctgggc
     3541 aaggcgcccg cgcccctgca acagcgaatc atattcgctg cgacgaacgg aaagatcgcg
     3601 cgccgcctcg ttcatgacct cggtcgcatc ctcgcgaatg tcgcgcagcc gctcgatcag
     3661 ttcgcttcgc gccgaggcga attgcttggt cgcctcgacc cagcgccgct caagtcccgc
     3721 atagcccatg tagggatcga agaacatcag accgtcggcc atcgcgatca gcgagaagat
     3781 caggccgatc gagaagaaga cccaggagtt gatgtcggtg agcgcgtagg gcgcggtcag
     3841 caggcgatgc agcacaacct cgccgacgtc gccgctgacg gcgacggccg gcggaatttc
     3901 gcgcagatgc gatagcgtga ggttgagcgc gatcgcaaag gcaagatagg cgaggaacga
     3961 caacagaccg atcagcttca ggaagaagtt gcggcgattg atcaggcgga tcagcaccat
     4021 gccccagaag aaactcgcaa ggatgttaag cgcggcaaag gttaccgcct gcgccgcgcc
     4081 gccgagcaag ccgccgacat tggctttcgc aagaaagctg ccgttgatca ccacttcggt
     4141 gatgaagagg atggcgagca cgccgatctt caggatgatc tttccggggc tcgcaaggcg
     4201 cgccggccgc tcgatgcgat ggcgcgcgcg aaagcgctcg cgctcgatct cgctctcgtt
     4261 gaggcggcgg cgcagcacga acaattcgtc acgcccgacg gtcgcctcgg cgcggaaatc
     4321 gccgacagcc tcggg
//
LOCUS       DATLJT010001366         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_112852,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001366 DATLJT010000000
VERSION     DATLJT010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112852"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VM910_31310"
     CDS             complement(<1..917)
                     /locus_tag="VM910_31310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HVI67030.1"
                     /translation="MVTSRRTRSNKKSTRPASGRKAAARHRVRASKASSDKTKWVYRF
                     GNGKAEGRADMRNLLGGKGAGLAEMANLGLPVPPGFTITTAVCSHYYENGKTYPKNLE
                     HQVAAALAEVGRITGKTFGDSGNPLLVSVRSGARASMPGMMDTVLNLGLNDATAEALA
                     KKSGDRRFAYDSYRRFITMYSDVVLGLGHHHFEEILDDHKDKNGYTLDTDLSADDWVK
                     LVARFQERIEEEHGEPFPQDPQQQLWGAIGAVFGSWMNQRAITYRRLHHIPESWGTAV
                     NVQAMVFGNMGETSATGVAFTRNPSTGDK"
     gene            complement(1010..1165)
                     /locus_tag="VM910_31315"
     CDS             complement(1010..1165)
                     /locus_tag="VM910_31315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HVI67031.1"
                     /translation="MTITVAHVTPLVALIAGILILLVPRLLNYVVAIYLIIVGLVGLN
                     GIYHFIR"
     gene            complement(1221..>2226)
                     /gene="glyS"
                     /locus_tag="VM910_31320"
     CDS             complement(1221..>2226)
                     /gene="glyS"
                     /locus_tag="VM910_31320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVI67032.1"
                     /translation="SGQTLSPDDLQREQDAYRRQVGRAARLAKADLLTEVVGEFPELQ
                     GLMGKYYALAQGEDASVAAACEEHYKPLGPADRVPTDPVSVAVALADKIDTLVGFWSI
                     DEKPTGSKDPYALRRAALGVIRIVLTSNLELSPRGMFGMHVRRFRPEMSDTEISNAGD
                     SFVDFFDERLKVQLREQGARHDLVDAVLYLRGTEASIVSIVRRVEALGKFLDTEDGKT
                     LLAGYKRATNIIRIEERKDGRTYSGWPEPSLYREKEEKALASAIDTAKGEAAAAVARE
                     DFEAAMRAMAKLRPHVDAFFDKVTVNVEDKQLRENRLNLLNEIRAATGTVADFSCIEG
                     "
BASE COUNT          400 a          680 c          712 g          434 t
ORIGIN      
        1 cttttgtcgc cggtggacgg attgcgggtg aacgccacgc ccgtggccga ggtctcgccc
       61 atattgccga acaccatagc ctgcacattg accgcggtgc cccagctctc cgggatatgg
      121 tgcaggcgcc gataagtgat ggcgcgctgg ttcatccagg agccgaacac ggcgccgatc
      181 gctccccaca attgctgctg cgggtcctgc gggaatggct cgccgtgctc ctcttcgatc
      241 cgctcctgaa agcgggcgac cagcttgacc cagtcgtcgg ccgaaagatc ggtgtcgagc
      301 gtgtagccgt tcttgtcctt gtgatcgtcg aggatttcct cgaagtggtg gtgaccaagg
      361 ccaagcacca cgtcggaata catggtgatg aagcggcggt acgaatcata agcgaagcgc
      421 cgatccccgg atttcttcgc cagcgcctcg gcggtggcgt cgttgaggcc gagattgagc
      481 accgtatcca tcatgccggg catcgacgcg cgcgcccccg aacgcaccga gaccagcagg
      541 ggattgccgc tgtcgccgaa ggtcttgccg gtgatgcggc cgacctcggc gagcgcggcg
      601 gcgacttggt gctcgaggtt cttcgggtag gtcttgccgt tctcgtaata gtggctgcag
      661 accgcggtgg tgatggtgaa gcccggcggc accggcaagc cgagattggc catctcggca
      721 aggccagcgc ctttgccgcc gagaaggttg cgcatgtcgg cgcggccctc ggccttgccg
      781 ttgccgaagc ggtagaccca tttggttttg tcggaagatg ccttcgaggc gcgcactcga
      841 tggcgcgccg cggccttgcg gccggatgca gggcgggtcg attttttgtt cgatcgagta
      901 cggcgcgacg tgaccatgga accacgaact tcaatgcgcc cggcccctaa tgatcaccgc
      961 cgcgggcgcc gacgcaaggg cgcagtttgg tcggccggcg gcgggcgtat cagcggatga
     1021 agtgatagat gccgttcaat ccaacgaggc cgacgatgat gaggtagatg gccacaacgt
     1081 agttcaacag ccgcggcacg agcaaaatca gaatgccggc gatgagggca acgagcggcg
     1141 tgacatgggc aacggtgatg gtcatgagga tctccttggg cgggtagatg aactacaaaa
     1201 cgcggtgaca gttccgagaa tcacccctcg atgcacgaga aatcggcaac cgtgccggtc
     1261 gccgcgcgaa tttcgttgag caagttgagg cggttctccc gtagttgctt gtcctcgacg
     1321 ttgaccgtca ccttgtcgaa gaacgcatcg acgtgcgggc gaagcttcgc catggcgcgc
     1381 atggcggcct cgaagtcctc gcgtgcgacg gcggcggccg cctccccttt cgcggtgtcg
     1441 atcgcgctcg ccagcgcctt ttcttccttc tcacgataaa gcgatggctc aggccagccg
     1501 gaatatgttc ggccgtcctt cctctcctcg atgcggataa tgttggttgc gcgtttgtag
     1561 ccagcgagca gagtctttcc gtcctcggtg tcgaggaatt ttcccagcgc ctcgacgcgg
     1621 cgcacgatcg aaacaatgct tgcctcggta ccgcgcagat aaagcacggc gtcgacgaga
     1681 tcgtgtcgcg ctccttgttc acgcaactgt accttgaggc gctcgtcgaa aaaatcaaca
     1741 aaactgtcgc ccgcgtttga gatttcagta tcggacattt ccggccgaaa ccgcctcacg
     1801 tgcatcccga acataccgcg cggtgagagt tcaagattgc tggtcaatac tatgcggatc
     1861 actcccaagg cggcgcggcg gagggcgtag ggatctttcg aacccgtcgg cttctcgtcg
     1921 atcgaccaaa acccgaccag cgtgtcgatc ttgtcggcga gcgcgacggc gaccgagacc
     1981 ggatcggtcg gcacgcggtc agcggggccg agtggcttgt aatgctcctc gcatgccgcg
     2041 gcgacgcttg catcctcgcc ctgcgccagc gcgtaatatt tccccatcag cccctgcagc
     2101 tcggggaatt cgccgaccac ctccgtgagc aaatccgctt tggcgagacg cgcggctcgc
     2161 ccgacctgcc ggcggtaagc atcctgctcg cgttgcagat cgtcgggcga aagtgtttgc
     2221 ccggag
//
LOCUS       DATLJT010001367         3901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1263943,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001367 DATLJT010000000
VERSION     DATLJT010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263943"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VM910_31325"
     CDS             complement(<1..1394)
                     /locus_tag="VM910_31325"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HVI67033.1"
                     /translation="MAPTVDPAWILVDEGFSLAREHELESLFAIGNGYAGSRGSLAEG
                     SALSAPATFLAGVFDSQVCSVTGLVSTADWTRLSATVNGRLLRLDQGHSLEHRRVLDM
                     RQGILWREWRHQNAAGEITRLKMLRLASLADRHLLVQCATITPENYSGSVSIDAALTG
                     TVTQVTGSGTTVAMAVARRVVDSAGHCMSSTEVVVGQLSLEVRLGETYRLDRVVAVHT
                     SRDTDEPREMAGRQVKHAIEDVNGVIAEHREAWLTRWKASDLRIEGDAAAQRALRFAI
                     YHLSSASNPEDERVSIGARGLSGTAYKGHVFWDTDIFLLPFFILTYPEAARALVMYRY
                     YTLAGARAKAARLGYRGALYAWESADTGEEMTPPFVVSPDGGIVRILTGEQEQHISAD
                     VAFGVWKYWEATGDEGFLVDAGAEILIETARFWASRAELEEDGRYHIRGVIGPDEYHE
                     KVDDNAYTNGLAQW"
     gene            2235..2594
                     /locus_tag="VM910_31330"
     CDS             2235..2594
                     /locus_tag="VM910_31330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HVI67034.1"
                     /translation="MAIQSEPTANRLTAIAGILGAIAASSCCVLPLLFASLGISGAWI
                     GNLTALAPYQPLALTVAVLGYGFYSVYWRPRQACSDGTCERPLPSRNVKLGLWLGTIL
                     VAVALIFPRIALVLAGY"
     gene            2615..2896
                     /locus_tag="VM910_31335"
     CDS             2615..2896
                     /locus_tag="VM910_31335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HVI67035.1"
                     /translation="MKKLIVVAAMTALGTGAAQAAERTVKFSVDKMYCEACPLTVSKA
                     MQGVKGVKSAKIDYKTKTAVVVFDDSAATVDAIAAASTNAGYPARPAAN"
     gene            3020..3268
                     /locus_tag="VM910_31340"
     CDS             3020..3268
                     /locus_tag="VM910_31340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HVI67036.1"
                     /translation="MILESVITCPHCATAKAETMPTDACQFFYTCTGCGATLRPKPGD
                     CCVFCSYGSVPCLPIQAERSGEMGAASRCATWPSWQTI"
     gene            3754..>3901
                     /locus_tag="VM910_31345"
     CDS             3754..>3901
                     /locus_tag="VM910_31345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67037.1"
                     /translation="MKTYDLVIIGSGTAAQVASSRVRAAGWSVALVDHRPFGGTCALR
                     GCDPK"
BASE COUNT          776 a         1174 c         1161 g          790 t
ORIGIN      
        1 ttccactgcg ccagaccatt ggtgtacgcg ttgtcatcta ccttctcgtg gtattcgtcg
       61 ggtccaatga ctccacgaat atggtagcgg ccatcttctt cgagctcggc gcggcttgcc
      121 cagaagcgtg cggtctcgat caggatctct gcgcccgcat ccacaagaaa gccctcgtcg
      181 ccagtagctt cccagtactt ccacacgcca aaggcgacgt ctgcgctgat gtgttgctcc
      241 tgctctcccg tgagaatccg cacgatcccg ccgtcggggg agacgacgaa tggaggcgtc
      301 atctcttcgc cggtatcggc cgactcccat gcgtagaggg cgccgcgata cccaagccga
      361 gcggccttgg ctcgggctcc agcaagcgtg tagtagcggt acatgacaag cgcccgcgcc
      421 gcctcgggat aagtgagtat aaagaacggc agcaagaata tgtcggtgtc ccagaacacg
      481 tgtcccttgt atgcagttcc gctcaggcca cgggcgccaa tggacacgcg ttcgtcctcc
      541 gggtttgacg cactcgacag atgataaatg gcaaagcgca acgcgcgctg agccgcagca
      601 tcgccctcga tccgaagatc agacgctttc catcttgtca gccaggcctc acggtgctcg
      661 gcgatgacgc cattcacgtc ctctattgcg tgcttgacct gcctgcccgc catttcgcgc
      721 ggctcgtccg tgtccctgga agtgtgcacg gcgaccaccc gatcgagccg gtatgtttcg
      781 cccagcctga cttcgaggct tagctggcca acaaccactt cagttgaact catgcagtgg
      841 ccggcggaat ccacgactcg gcgcgcaacc gccatggcga ccgtcgtgcc gctacccgtg
      901 acctgggtta cggtgccggt cagtgcggcg tcgatagata cacttccgct gtagttttcg
      961 ggcgtgattg tcgcacactg aaccaggagg tgccggtcgg ccaaagaggc gaggcgtagc
     1021 attttcagcc gcgtgatttc accggctgcg ttctgatgcc gccactcccg ccagaggatg
     1081 ccttgccgca tgtcgaggac gcggcgatgc tccaggctat ggccctgatc gagccgaagc
     1141 aggcggccgt tgaccgtggc ggacagacgg gtccagtccg ctgtcgacac aaggcccgtt
     1201 acggaacaaa cctgagaatc gaataccccc gccaagaaag tcgcaggtgc tgagagcgcg
     1261 ctgccttccg caagcgagcc acgcgatcct gcatagccat tgccgatcgc gaacaacgac
     1321 tccagctcat gctcgcgcgc gagcgaaaag ccttcatcga cgaggatcca cgcaggatcg
     1381 acggtaggtg ccaccggcaa atccgcggga tgcagcgcag ccgggccagc attcgattgc
     1441 ctaggccccc cgccgacagg atcgaccata tccttccacc ctggcgtgtc tgtcaggccc
     1501 ccgaccgaaa cgcgactggc gttgcgcgtc aaggccagca gaagattggc gcagctcgca
     1561 atacagacaa catgaccgtc gctgacggtt cccgaacgag tatcaaggaa ttttttttcg
     1621 aggaacaggg aattaataga gggagcagcg agaccggccg aaccaatttt ggggcgccct
     1681 cgaggggtga cgtaacgaga tagtcttctg agcgcgcgcc ggaggggtta tcacgcagcg
     1741 cgagaagcgg atcatatatg ccacgcagcg ccgccagcgg atcatagcag cgccgagcgc
     1801 cgccaaacga aaatccaatg gcgcatcatt ttccatggga cttccccttg ggactttctt
     1861 aagcccgagg ggcttgtgtg gtttgtgcgg cgttgcaagc attcgtttga tcgctgattc
     1921 gcggcgacgc tgaatctctt caggggagag ctcttcggta ggtcgcttca tgaaaaaaac
     1981 cgttgtcccc gcagttcgag gcgctgaaaa tatatacgaa cgccgagagg gaccgcgtct
     2041 actataagcc ctcgcgactg gaggcacggc ggcgcctatt ccgcatattt cggcagatcg
     2101 tcggtgctca acaagaatat cgtcgtgcaa gaatggcttc gttgaatagc tcgggtttgt
     2161 ggaccgcgag gacgaacgcc attcccggcg gtagcggttg agaatattcc gggtgccaat
     2221 aaggagcctt ggctatggcc attcaatccg aacctacggc gaaccgactg acggcgattg
     2281 caggcatctt gggtgcgatt gccgcttcgt catgctgcgt gctgcctctg ctcttcgcaa
     2341 gtctcggtat aagtggcgcg tggatcggca acctgacggc gctcgcccca taccagcccc
     2401 ttgcgctcac cgttgctgtg cttggctacg gcttctactc cgtctactgg cgaccgcgac
     2461 aggcctgttc tgatggtacg tgcgaacggc cgctaccgag ccgtaacgtg aagcttggct
     2521 tgtggctcgg aacgatcctc gttgcggtcg ccctgatctt cccgcgcatc gccctcgtgc
     2581 ttgcaggata ttgaaacaaa gcaaggagaa caccatgaaa aagctgatcg tagtcgcagc
     2641 catgacggcc ctcggaacgg gcgcagctca agccgcggaa cgcacagtga agttcagcgt
     2701 cgacaagatg tactgtgaag cctgccccct cactgtcagt aaagccatgc aaggggtgaa
     2761 gggcgtgaag tcagcaaaga tcgactataa gaccaagacc gccgtcgtcg tctttgacga
     2821 cagcgcggcc actgtggacg cgatcgcagc ggcaagcacc aatgctggtt atcccgcccg
     2881 gccggccgca aactgaaagc ggttcaggtc aagcaaggcg acgcaggcgc cgtggctacg
     2941 atcaacttct acgatgtggt agcgagcgtg gctatccgac cgaagcctcc gatatcggct
     3001 gcatgcttta ggaagaacca tgatccttga atctgtcatt acatgtccac attgcgccac
     3061 cgcgaaagcg gagaccatgc ccaccgatgc ctgccagttt ttctatacct gcaccggctg
     3121 cggcgcgacg ctgcggccga agccgggcga ttgttgcgtg ttctgttcct acggctccgt
     3181 tccgtgcctg ccgatccagg ccgagcggtc gggtgagatg ggcgccgctt cgcgctgtgc
     3241 cacatggcca tcatggcaaa cgatatagtc caaagctcgc acgactggct cagcagccct
     3301 cgcacgagcg cgttggcatt gtggattccg aaagcgggca tcgttgcggc gcttttcttt
     3361 cctccgccgg cgcgaaccag gatgtggatc atcgcgctcg tctggatggg cactgcgtgc
     3421 attctgaatg caaggcggtg cgggcggacc cactgccgct acacggcccc ttattatctc
     3481 gcgatgatcg caccggtgct cgtgcttgct tcgggtgtcg tttccgtcga cttctatgca
     3541 tggctttcat tggcagttct cattcttggc ggaagcatga tcatttggtg ggccacggaa
     3601 agggcgtggg gaaagttcct cgcggcgcga cgatagcttt tgatcatcag gtcaatagcc
     3661 gaaaactaat gtacatactt cggtactgtt cctcataatc gtccagcggg agccacgcag
     3721 cgcattgaac gtgtgatcgg aggagaccac gttatgaaga cctacgacct tgtgatcatc
     3781 gggtctggca ctgccgcgca ggtggcaagc tcgcgagtgc gtgccgcagg gtggtctgta
     3841 gcattggtcg atcatcgtcc tttcggcggg acttgcgccc tgcgcggctg cgatccgaag
     3901 a
//
LOCUS       DATLJT010001368         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2411885,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001368 DATLJT010000000
VERSION     DATLJT010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411885"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VM910_31350"
     CDS             <1..337
                     /locus_tag="VM910_31350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67038.1"
                     /translation="AGIRDFVRNDVAYLRRPPVMNNIESLEPSAEAAVNNVNTLIQRV
                     AGTSLSEIEHLISELESLREVLHAEGQRVQREISGYAQLSQAAMKSTRMIAENVAQWK
                     RTTDGLRNS"
     gene            487..624
                     /locus_tag="VM910_31355"
     CDS             487..624
                     /locus_tag="VM910_31355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67039.1"
                     /translation="MDSIQQEKPAPLPLRPAPPRVGTLMLRFVGLLFLAIAAMALVYM
                     R"
     gene            complement(668..811)
                     /locus_tag="VM910_31360"
     CDS             complement(668..811)
                     /locus_tag="VM910_31360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67040.1"
                     /translation="MADRSGLKLVGFIFATVTVAVMLTAAMVVKGYADGAYELEAAPI
                     ESQ"
     gene            1454..1747
                     /locus_tag="VM910_31365"
     CDS             1454..1747
                     /locus_tag="VM910_31365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HVI67041.1"
                     /translation="MFDFGDLFQWDRFITPTIIKTFYWLVIALICLLGLSGIFSGLAA
                     MAISPFGGFLVLLYSIASVIVGIVFSRIVAEFVLIVFRINEHLGAIRDQGGMR"
     gene            complement(1773..>2453)
                     /locus_tag="VM910_31370"
     CDS             complement(1773..>2453)
                     /locus_tag="VM910_31370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HVI67042.1"
                     /translation="SLEKRVDNLAKKAKGNDKEAKEQLDLVNRSLALLREGKPARLLE
                     RKAEEERSFAMLGLLTSKPVLYVCNVEEAAAKDGNAFSRQVFERAKEEGAVAVVISAK
                     IESEIATLSREERKEFLDTLGLEEAGLDRLIRAGYQLLDLITYFTVGPKEARAWTIHR
                     GTRAPAAAGVIHSDFEKGFIRAETIAYADYVALNGEAGARDAGKLRLEGKDYVVADGD
                     VMHFRFNT"
BASE COUNT          429 a          770 c          738 g          516 t
ORIGIN      
        1 ggccggtatc cgcgatttcg ttcgcaacga tgtcgcctat ctgcgccgtc cgccggtcat
       61 gaacaacatt gagtcacttg aaccgagcgc cgaggctgcc gtgaacaatg tcaatacgct
      121 gatccagcgc gtcgccggca cctcgctgtc ggagatcgag cacctgatct cggagctcga
      181 gagcctgcgc gaagtgctgc atgccgaagg ccagcgcgtc cagcgcgaga tctccggcta
      241 tgcccagctc agccaggctg cgatgaagtc cacccgcatg atcgcggaga atgtggcgca
      301 gtggaagcgc acgacggacg ggctgcggaa tagttgatcg gcgttgtcag ccgttcgatg
      361 ccgccgcccc gtgttcgcgg aggcggcggt ttttgattcg gccgaaacct gtgatttgaa
      421 cttgatcctc gttgaccgcg gccaacggca cgcgccttac tgtggcgtta ctatcagggg
      481 attttgatgg atagcatcca acaggaaaag cccgcacctt taccgctgcg cccggcgccg
      541 ccgcgcgtgg gcaccctcat gctccggttt gtcggactgc tgtttctcgc catcgccgcg
      601 atggcactgg tctatatgcg ctaagtcgcg gcgcggatga accgtttctg atttgaactg
      661 ttgctgatta ttggctttcg atcggcgccg cttccagttc ataggcgccg tcggcataac
      721 ccttcaccac catcgcggcc gtcaacatca ccgcaaccgt cacggttgcg aagatgaagc
      781 ccaccagttt caagccgctg cgatccgcca tcgtcatccc cgccgaatcg tttgcgcgcg
      841 atctccgcgc aaacgcgttt ctgcgcttgt tgcgagagag aaccggtccc cacctttccg
      901 gatcgagcgc gaacgggccg catcatggcc ccgacatgtt caaaatgcgt tagcaaaaaa
      961 atcgttccac gcactcgtgg gtatgattga acaaggaaac gattcggtaa ccttgatttg
     1021 cggttaccgc gtcaggcccg cgattcccaa atgcgcgata cgcggcgcgg ggcgaaggta
     1081 ccgaaagggc cgggtgaaag gtcttcgaag gtcagggtca tctacagcat tgcggcagcc
     1141 cggcccgatt gtggccgcac tttccggcaa tctcaggcga ctcggtcgca cggcccgtgc
     1201 tcgaatcttg ctggggacgg caccggccgg ccacgctgtg gcgtaacgga tagtccgatt
     1261 ccgacattcg cattcccatt gcggcgggca tctttgcgaa tgccggaaaa ggaccgctcg
     1321 caaatatcgc ttgccagtgg agttcggatt tgacattcgc ttttgccgag cctgtggacg
     1381 gacgcctggc gaacgtcaaa tccactccac tagtctaagc gcagcattcg acttgccggg
     1441 ggagagccga ccgatgtttg attttggcga cctgtttcaa tgggaccgct tcatcacgcc
     1501 gaccatcatc aagaccttct actggctggt gatcgccctg atctgcctgt tgggcctgtc
     1561 cggcatcttc tcgggcctgg ctgcgatggc aatcagccct tttggcggct tcctggtgct
     1621 gctctattcg atcgccagcg tcatcgtcgg catcgtgttc tcgcgcatcg tcgccgaatt
     1681 cgtgctgatc gtgttccgca tcaacgaaca tctcggcgcc atccgcgacc agggcggcat
     1741 gcgataggcg acattgtcat atcgggcgcg tatcaggtat tgaaccggaa atgcatgacg
     1801 tcgccgtcgg cgacgacata atccttgcct tcgaggcgga gcttgccggc atcgcgggcg
     1861 ccggcttcgc cgttgagcgc gacgtagtcg gcataggcga tggtctcggc gcgaataaag
     1921 cccttctcga aatcggagtg gatcacgccg gcggcggccg gcgccctggt gccgcggtgg
     1981 atggtccagg cgcgggcctc cttcggtccc acggtgaaat aggtgatgag atcgagaagc
     2041 tggtagccgg cgcgaatcag gcggtcgagg ccggcctcct ccagtcccag cgtgtccagg
     2101 aattccttgc gctcctcgcg cgacagcgtc gcgatttcgg attcgatctt ggccgaaatc
     2161 acgaccgcga ccgcgccctc ctctttcgcg cgctcgaaca cctgccgcga gaaggcattg
     2221 ccgtctttcg cggccgcctc ttcgacattg cagacgtaga gcacgggctt cgaggtcaat
     2281 agcccgagca tggcgaagct gcgctcctcc tccgccttgc gctcgagcag ccgcgccggc
     2341 ttgccctcgc gcagcagcgc cagcgagcgg ttgacgaggt cgagctgctc cttggcctcc
     2401 ttgtcgttgc ccttcgcctt cttggcgaga ttgtcgacgc gcttctcgag act
//
LOCUS       DATLJT010001369         1947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_344285,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001369 DATLJT010000000
VERSION     DATLJT010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344285"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1251
                     /locus_tag="VM910_31375"
     CDS             40..1251
                     /locus_tag="VM910_31375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HVI67043.1"
                     /translation="MFEGHDPYLVALSVVVAILGGYTGFALAARVRSAPGGSRRALLA
                     GAATFLALGIWTMHFVGMLAAPIPADTVYLVLPTMISFLICALVVGISLFFVSVGEPS
                     LLRVMSSAVLLGLGVTSMHYVGIHGLAGNFAIEHDWPVVLLSVVVAIVTAYGGLRAFL
                     ARHDGIRLIASAIAFGVAVSGMHYTATYGMHFVACSPEVAQAHLNRLAASPQILSIVV
                     SVLCFVIAAGFLLSLVPDPRRQMIASPAEVPVAVAGAALPLAAPVNLSSLRLMPAAPL
                     GGIGQPPRPAPAPRLPVEGVNGTHFIDAADVRSVRADAHYTLVHDGTRERMCPWSISQ
                     AEAQLDPGLFVRVHRSHIVAISHVSFVRREGDGAVIELDGPTPHRVPVSRAKIAEVKA
                     RLGLAKRHASH"
     gene            1554..>1947
                     /locus_tag="VM910_31380"
     CDS             1554..>1947
                     /locus_tag="VM910_31380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI67044.1"
                     /translation="MIPGSFNYHRPTSVADAVKLLSTLGEEARPLAGGHSLVPMMKLR
                     LATPGHLVDLHGIAGLKGILQDGNRIVIGAMTTQHELLQSDEIAKSLPILHEAAGLIA
                     DPQVRYCGTLGGNVANGDPGNDMPALMLT"
BASE COUNT          297 a          636 c          631 g          383 t
ORIGIN      
        1 ggaggacggc ggtccgtgga gacggacggc cgaaagatca tgttcgaagg acacgatccc
       61 tatcttgtcg cgctctcggt ggtggtcgcg atcctgggtg gatatacggg cttcgcactc
      121 gccgcacgtg ttcgtagcgc gccgggcggc agccgccgcg cgctgctggc gggggctgcg
      181 acgtttctcg ccctcggcat ctggaccatg catttcgtcg gcatgcttgc cgcgccgatt
      241 ccggccgaca ccgtctatct cgtgctgcca acgatgatct cgttcctgat ctgtgcgctg
      301 gtggtgggca tttccctgtt tttcgtcagc gtcggcgagc cgtcgctgtt gcgggtgatg
      361 tcgtcggccg ttctgctcgg gctcggcgtt accagcatgc attatgtggg aattcacggg
      421 cttgcgggca atttcgccat cgaacacgac tggccggtag tgctgttgtc ggtcgttgtc
      481 gcgatcgtca cggcctatgg tggtctgcgc gcgttcctgg cccggcatga tggcattcgc
      541 ctgattgcaa gcgcgatcgc gtttggcgtc gcggtgtcgg gcatgcatta caccgcgaca
      601 tacggcatgc atttcgtagc ctgctcgccg gaggtggcgc aggcccatct gaaccggctc
      661 gctgcatcgc cgcaaatcct gtcgatcgtg gtgtcggtgc tctgctttgt gatcgccgca
      721 ggctttttgc tgtccctggt gcccgatccg cgccggcaaa tgatcgcttc gcccgcggag
      781 gttccggtcg cggtcgccgg cgcggcattg ccgcttgccg ctccggtcaa cctgagttcg
      841 ctacggctga tgccggcggc gccgctcggc gggattggcc agcccccacg accagcgccc
      901 gcaccacggc ttccggtcga gggtgtcaat ggcacgcatt tcatcgatgc cgccgatgtt
      961 cggagcgttc gcgccgacgc gcactacacc ctcgttcatg atggtacgcg cgagcggatg
     1021 tgcccctggt cgatttcgca agccgaggcg cagcttgacc ccggcctgtt cgtgcgcgtc
     1081 catcgcagcc acatcgtggc gatttcccat gtctcctttg tccgcaggga gggtgacggc
     1141 gccgtgatcg agctcgacgg gccgacgccg catcgcgtgc ccgtgagccg cgccaagatc
     1201 gccgaggtca aggcacgcct tgggctcgcc aagcgccacg cttcacacta gccgggcgga
     1261 tttgacgttc gcagccgccc gcgaattagg tcggaagacc tgacgcaatt cgtgcgctgc
     1321 cggccgctct tcgtgcgaaa attcgaaatc cgtgagggcg ctgcctgcat cgccttgcgc
     1381 acgcaagctt ccgggcagag cgtccccgcc aacgccccgg cacgcaggcc gcagcgggtc
     1441 ccgggctttg gggccagaat gcgcgccaaa acacgcgcgc gtattccgca aaagtgggaa
     1501 ccggttttgc ggtcagaaca cgcgccacaa caacaagacg gatggaggag acgatgattc
     1561 ctggttcgtt caactaccat cggcccacaa gcgtggccga cgccgtcaaa ctgctgtcga
     1621 cgctgggcga ggaggcgcgt ccgctcgccg gcggccacag ccttgtcccg atgatgaagc
     1681 tccgcctcgc aacccccggc catctggtcg atctgcacgg gattgcggga ctgaagggca
     1741 tcctccagga cggcaacagg atcgtgatcg gcgcgatgac cacccagcac gagcttctgc
     1801 agtccgatga gatcgccaaa tcgcttccga tcctgcacga agcggccggc ctgatcgccg
     1861 atccgcaggt gcgctattgc ggcaccctgg gcggcaatgt cgccaatggc gatcccggaa
     1921 acgacatgcc ggcgctgatg ttgacct
//
LOCUS       DATLJT010001370         4064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_113512,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001370 DATLJT010000000
VERSION     DATLJT010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113512"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1452
                     /locus_tag="VM910_31385"
     CDS             169..1452
                     /locus_tag="VM910_31385"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVI67045.1"
                     /translation="MARPVFIIDGSRTPFLKARSGPGPFTPVDLAVQCGRPLLARQPF
                     PLDAFDQVILGCVNVIADEMNPARVAALRLGMGESMVAFTVQINCGSGMQSIDTGYRY
                     IREGISDLILAGGTEALSYAPLVWPQGGVRWFAGLAGAKGVGAKILALLKARPKDFKP
                     IIGLERGLTDPITELNMGQTAEVVGHLFGITREQSDAYAAESHKRLANAQAQGWLKNE
                     IEIAFARDGKFYDHDDGVRPDSTPETLAKLKPVFERPWGQVTAGNSSQITDGASWVIL
                     ASEDAVAKHGLQPKAAIVDSEWAALDPSIMGLGPVLSATALLKRNELAVQDVETWELN
                     EAFATQVLGCLASWNDDKFCREILDLPGAAGEIDRTKLNVDGGAISLGHPVGTSGNRI
                     VLHLINAMRRLGTRRGIATECIGGGLGGAMLIETV"
     gene            1455..3551
                     /locus_tag="VM910_31390"
     CDS             1455..3551
                     /locus_tag="VM910_31390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVI67046.1"
                     /translation="MDSRIMDVLADRVLALGPMPAASGAYHNFKLTRDGDGIAWLLFD
                     REGASANTLSADVMEEFDRILAELESQRPAGLVIRSAKKSGFIAGADVNEFRGVSDSS
                     IVEAAIGRAHAVIDRLEALRVPSVAVIHGFCLGGGLEVALACQSRIAIDNARFGFPEV
                     LLGLHPGLGGTVRFTHLVNPVQAMSLMLTGKTIDARRAKSLGLVDAICQERHVGNAVK
                     DAVFGRLKRARPGLSNTVLNSGPARGFLASRMRREAAKAAPPQHYPAPYGLIDLWEKH
                     GGDKEAMLAAERSSFAHLMVTPTAQNLIRVFFLREEMKKLAGSGNKIGHVHVIGAGAM
                     GGDIAAWCANEGMQVTLADMKAEPIAGAVKRAADLCGKIIRKPTEVRDALDRLMPDME
                     GEGVRNADLIIEAVPEKLELKQKVYASLEQKMKGSAILATNTSSIPLQDLRTNLQRPE
                     RLLGLHFFNPVSRLQLVEVVSHDGNDPVLLKEALAFVGAIDRLPLPVKSSPGFLVNRA
                     LTPYMLEAMVMLDERIDQRLIDAAAEQFGMPMGPIELADQVGLDICLDVGDMLRSKFG
                     DLLPPTPAWLREKVAKGELGRKSGKGFYVWKDGKADKQPLPETGPRPSDEMIDRLILP
                     MSNVCVACLREGIVDNADVVDGAMIFATGYAPFRGGPLNYARTRGVANVVSAMETLSQ
                     RFGGRFTPDAGWESFK"
     gene            3652..>4064
                     /locus_tag="VM910_31395"
     CDS             3652..>4064
                     /locus_tag="VM910_31395"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVI67047.1"
                     /translation="MTDTQIHASPSTDTEPCGDLCIRTLAMPADTNQNGDIFGGWLLS
                     QMDVAGGVFAAKTTKSRTVTVAIEAMNFRKPVYVGDLVSVYANLVRVGRTSVTVHLEA
                     WVLRRKESQSMILVTDGNFTYVSIDEQGKPQAVPKV"
BASE COUNT          752 a         1269 c         1319 g          724 t
ORIGIN      
        1 cgtcatcacc cagtccgaag ccgagaggct cgcagccgcg catgaggccg tcgcgaaagt
       61 gatcgatgtc gacgattttg ccccagaggc actgtcgccg atttacagga agcccgccga
      121 cgtgcatcag ttcttccagg aattgggcga gcagagggcg gcgagctgat ggcgcgacct
      181 gttttcatca tcgacggcag ccggacgccg ttcctgaagg cgcgctccgg tcccgggccg
      241 ttcacgcccg tcgatctcgc cgtccaatgc ggcaggccgc tgctggcgcg gcagccgttt
      301 ccgctggatg ccttcgatca ggtgatcctc ggctgcgtca atgtcatcgc cgacgaaatg
      361 aacccggcgc gcgtcgcagc actcaggctc ggcatgggcg aatccatggt cgccttcacc
      421 gtgcagatca attgcggttc tggcatgcag tcgatcgata ccgggtatcg ctacatccgc
      481 gaaggcattt ccgacctcat ccttgcggga ggcaccgaag cgttgagtta tgctccgctg
      541 gtctggccgc agggcggcgt gcgctggttt gcaggccttg ccggcgccaa gggcgtcggc
      601 gcaaagattc tggcccttct gaaagcgcgt ccgaaagatt tcaagccgat catcgggctc
      661 gagcgcgggc tgaccgatcc catcaccgaa ctcaacatgg ggcagaccgc cgaagtcgtc
      721 ggccacctct tcggcatcac gcgcgagcaa tcggatgcct acgcggccga aagccacaag
      781 cggctcgcga acgcgcaggc gcagggttgg ctcaagaacg agatcgagat cgcgtttgcc
      841 cgtgacggaa aattctacga tcatgacgac ggtgtgcggc cggactcgac gcccgaaacg
      901 cttgcgaagc tcaaaccggt gttcgagcgt ccatggggac aggtcaccgc cggcaattcc
      961 tcgcagatca ccgacggtgc gtcctgggtg atcctggcct ctgaagacgc ggtggcaaag
     1021 cacggcttgc agccgaaagc cgcgatcgtc gacagcgaat gggcggcgct cgatccctcg
     1081 atcatgggcc tgggccccgt gttgtcggcg accgcgcttt tgaagcgcaa cgagctggcg
     1141 gttcaggacg tcgagacctg ggaattgaac gaagcctttg ccacgcaggt gctcggctgc
     1201 ctcgcctcct ggaacgacga caagttctgc cgggagattt tggatcttcc cggcgccgcc
     1261 ggcgaaatcg atcggacaaa gctcaacgtc gacggcggtg ccatcagcct cggtcatccc
     1321 gtcggcacat caggcaatcg cattgtgctg catctcatca atgcgatgag gcggcttggc
     1381 acgcggcgcg ggatcgccac tgaatgcatc ggcggcgggc tgggcggtgc gatgctgata
     1441 gagacggtgt gatcatggat tcgaggatca tggacgttct cgccgaccgt gtgctcgcgc
     1501 tcggacctat gcccgcagcc agtggcgcct atcacaattt caagctgacc cgggacggcg
     1561 acggcatcgc ctggctcctg ttcgaccgcg aaggggcaag cgccaacacg ctgtcggccg
     1621 atgtgatgga ggagttcgac cgcatcctcg ccgagctcga gagccagcgg ccggcgggac
     1681 tcgtcatccg gtccgccaaa aagtcgggct tcatcgccgg tgccgacgtc aacgaattcc
     1741 gcggcgtcag cgactccagc atcgtcgagg ccgcgatcgg ccgcgcgcat gcggtgatcg
     1801 accgcctcga agcgctgcgc gtgccgagcg tcgccgtcat ccacggcttc tgtctcggcg
     1861 gcgggctcga agtcgccctt gcatgccagt cgcggatcgc gatcgacaac gcgcgcttcg
     1921 gcttccccga ggtgctgctc gggttgcatc cgggccttgg cggaacggtg cgcttcacgc
     1981 acctcgtcaa tcccgtgcag gcgatgagcc tgatgctcac gggcaaaacc atcgacgcgc
     2041 ggcgggccaa atcgctcggg cttgtcgacg ccatctgcca ggagcggcat gtggggaatg
     2101 ccgtgaaaga tgcggtgttc ggtcgcctca agcgcgcgcg gcccgggctc tcgaacacgg
     2161 ttctcaattc cggcccggcg cgcggctttc tcgcctcgcg gatgcggcgc gaagcggcca
     2221 aggccgcgcc cccacagcat tatcctgcgc cttacggcct gatcgacctc tgggagaagc
     2281 acggcggcga caaggaagcg atgctggccg ccgaacggag ctcctttgca catctgatgg
     2341 tgacgccgac cgcgcagaac ctgatccgcg tgttcttcct gcgcgaggag atgaagaaac
     2401 tcgcagggag cggcaacaag atcggccatg tccatgtcat cggcgccggc gccatgggcg
     2461 gcgacatcgc ggcctggtgc gccaatgaag gcatgcaggt cacgctcgcg gacatgaaag
     2521 cggagccgat cgccggcgcg gtcaaacgcg ctgccgatct ctgcggcaag atcatccgca
     2581 aaccgaccga ggtccgtgat gcgctggatc ggctgatgcc cgacatggaa ggtgagggcg
     2641 tgcgcaatgc cgacctcata atcgaggcgg tgccggaaaa gctcgaattg aagcagaagg
     2701 tctatgcctc gctcgagcag aagatgaaag gcagcgcgat tctcgccacc aacacctcga
     2761 gcattccgct gcaggacctt cgcaccaatc tgcagcggcc ggaaagactt ctcgggctgc
     2821 acttcttcaa tccggtgtcg cggctgcaac tggtcgaagt ggtcagccat gacggcaacg
     2881 atcccgtgct tttgaaagag gcgctcgcct ttgtcggtgc gatcgaccgg ttgcccttgc
     2941 cggtgaagag ttcgcccggt ttcctcgtca accgtgcgct gacgccctac atgctggagg
     3001 cgatggtgat gctggacgag cggatcgacc agcgcctgat cgatgcggcg gccgaacagt
     3061 ttggcatgcc gatgggaccg atcgaactcg ccgaccaggt cgggctcgac atctgtctcg
     3121 acgtcggcga catgctgcgt tcgaaattcg gcgacctcct gccgcccacg cccgcctggt
     3181 tgcgcgaaaa agtggccaag ggcgaattgg gccgcaagag cggcaagggc ttctatgtct
     3241 ggaaggacgg caaggccgac aagcagccgc tgccggagac cggcccgcga ccatcggacg
     3301 agatgatcga tcgcctgatc ctgccgatgt cgaatgtctg cgtcgcctgc ctgcgcgagg
     3361 gtatcgtcga caatgccgat gtggtcgacg gcgccatgat ctttgccacc ggctatgcgc
     3421 cgttccgcgg cggtccgttg aactatgcgc ggacgcgagg cgtggcgaat gtcgtctcgg
     3481 cgatggaaac gctttcgcag aggtttggcg ggcgctttac tccggatgcc ggttgggaaa
     3541 gtttcaagtg aatttccgct atcgcaggat gggttgagca aagcgaaacg cgtcactgca
     3601 cgtcaatatc tgatgggttt tcgttcgacc catccagcac ggaaggaagg catgacagac
     3661 acccagattc acgcgtcgcc gagcacggac acggagccct gcggcgatct ctgcatccgc
     3721 acgctggcga tgccggccga caccaatcag aacggcgaca ttttcggtgg ctggctgttg
     3781 agccaaatgg acgtcgccgg cggtgtgttc gccgccaaga ccaccaagtc gcgcacggtg
     3841 acggtcgcga tcgaggcgat gaatttccga aagccggtct atgtcggcga tctcgtctct
     3901 gtctacgcca acctcgtgcg cgtcgggcga acctccgtca ccgtgcatct ggaagcctgg
     3961 gtgctgcggc gcaaggagag ccagtcgatg attctcgtga ccgacggcaa cttcacctat
     4021 gtctcgatcg acgagcaggg caagccgcag gcggttccca aagt
//
LOCUS       DATLJT010001371         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2872114,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001371 DATLJT010000000
VERSION     DATLJT010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872114"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VM910_31400"
     CDS             <1..388
                     /locus_tag="VM910_31400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI67048.1"
                     /translation="DQLEVYAPVASQVYQRNVEPGEYVSPGVPLITLIDLADVWIHFD
                     LREDLVKGLKVGDRFDVHIPALDDRRITVEVKLIATKGEYASWRATRASGDFDLRTFS
                     IRAYPVQPVPELRPGMSAYLDWQSRQ"
     gene            385..>2253
                     /locus_tag="VM910_31405"
     CDS             385..>2253
                     /locus_tag="VM910_31405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67049.1"
                     /translation="MSLASKPGFRLVVQRECRWLLRDRVALLLIFGVPLFAFVVLTTV
                     FSHPVIRGLGVTVVDEDKSDASRALVEYIAASPSLHIVDRAGTLSTAVHDIRSGKAIS
                     AVYIPPDFERDLKADRRPQVVGFYNQQFLTPSGIASSGLSDALSAAAAVAAPAKRAAP
                     APASIGTMTAETIALVNPQKNYAQFLLRALLPTIIHVVITLAAGYSVGSEFRRRDARA
                     WLESAGGNPVVALVGKLTPLFCIFVLIMLAEPFVLEGVLQIPFKGDVPLLVAAGSLLI
                     IAYLSLGALLQLLAGDLATGLGLAGLIASPAFGYAGVGFPTIGMNTFAQVWSSILPLR
                     WYMAVLLGQAARGLPVSDSAVPFAALAGLAVLFAILALLRMASLTRKGWFETPRPAEQ
                     GDSDPPPRGIGGAFASEWRRVLGTRSAFTLLFLAPLIYGIYYPQPYLNQILRKLPIAV
                     VDNDLSDLSRQIVETLDASGALRVAVRARTLAEARAAIDRGEAFAAVDIPSGTERDVL
                     KGITAHIPIYADATYLFIFRSTASGVATAIGTLTSNLVSRGARSDGSLIQAKLASMSP
                     ADVLLQPIFNPVGGYASYVVPAAFILILQQTLLIGAAMLTRAALAKGGGAFAGVFGR"
BASE COUNT          351 a          762 c          682 g          458 t
ORIGIN      
        1 cgatcagctc gaggtctatg cccccgtcgc ttcgcaggtc taccagcgca atgtggagcc
       61 gggcgaatat gtatctccgg gcgtgccgct cattaccttg atcgatctgg ccgacgtctg
      121 gatccacttc gaccttcgcg aggatctggt gaagggcctg aaggtcggcg atcgatttga
      181 cgttcacatt ccagcgctcg atgaccgccg catcacggtc gaagtcaagc tgattgccac
      241 caagggcgaa tatgcgagtt ggcgggccac gcgagcctcg ggcgatttcg atctcaggac
      301 gttttcgatc cgcgcctatc cggtccagcc ggtgcccgag ctgcggcctg gaatgagcgc
      361 ctatctcgac tggcagtcgc gacaatgagt ctggcatcca agcccggatt ccggctggtc
      421 gtgcaacgcg aatgccgctg gctgcttcgc gatcgagtgg cgttgctgct gatcttcggc
      481 gtaccgcttt ttgcgttcgt ggttctcacc accgtgttca gccatccagt gatccggggg
      541 cttggggtca ccgtcgtcga cgaggacaag tcggatgcat cgcgcgcgct ggtggaatat
      601 atcgcggcat caccgagcct gcatatcgtc gaccgcgccg gcacgctctc cacggccgtg
      661 cacgatatac ggtcgggaaa agcaatctcc gccgtctata tcccgccgga tttcgagcgc
      721 gacctgaaag ccgaccgccg gccacaggtc gtgggcttct ataaccagca gttcctgacc
      781 ccgtccggca ttgcatcctc gggcctgagt gacgcgctgt ccgccgcggc agccgtagcc
      841 gctcccgcca agcgcgccgc gccggcgccg gcctcgatcg gaacaatgac cgcggagacc
      901 atcgcactgg tcaacccgca aaagaactat gcgcagttcc tgctgcgtgc gctgctgccg
      961 acgatcattc acgtcgtgat cacgctcgcc gccggctatt ccgtcggctc cgaattccgc
     1021 cgtcgcgacg cgcgggcctg gctcgaaagt gccggaggca atccggtcgt cgcgctggtc
     1081 ggcaagctca cgccgctgtt ctgcatcttt gtcctgatca tgttggccga gccgttcgtt
     1141 ctcgaaggcg tactgcaaat ccccttcaag ggagacgtgc cgctgctggt cgccgccggc
     1201 tcgctcctga tcattgccta tctgtcgctt ggcgccttgc tacagctcct ggccggcgac
     1261 ctcgcgacag ggctcggact tgccggcctg attgcctctc ctgcattcgg ctacgcgggc
     1321 gtcggctttc ccacgatcgg catgaacacc tttgcgcaag tctggagctc catcctgccg
     1381 ctgcgctggt acatggccgt cctgctggga caagccgcgc ggggattgcc tgtctctgat
     1441 tccgccgttc cttttgctgc gctcgcgggc ctggcggtgc tgtttgccat cctcgcattg
     1501 ctgcgcatgg cgagcctcac gcgcaagggc tggttcgaga cgccgcgacc tgccgagcaa
     1561 ggcgacagcg acccgccccc acgcggcatc ggcggcgcct tcgcgtcgga gtggcggcgc
     1621 gtgctgggaa cgcggagcgc cttcaccctt ttgttcctcg cccccctgat ctacggcatc
     1681 tattatcccc aaccctatct gaaccagatc cttcgcaagc tgcccatcgc agtcgtcgac
     1741 aatgatctga gcgatctcag ccgccagatc gttgagacgc tggacgcgag cggcgcattg
     1801 cgcgttgcgg ttcgcgcgcg aacgcttgcc gaggcgcgtg ccgccatcga tcgcggggag
     1861 gcctttgccg ccgtcgatat cccttccggc accgagcgtg acgtgctcaa gggcatcact
     1921 gctcacattc cgatctacgc ggatgccacc tacctgttca tattcagatc gaccgcaagc
     1981 ggcgtcgcca cggccatcgg cacattgacc tccaatctcg tttcgcgggg cgcacgctcc
     2041 gatggaagcc tcattcaggc gaaattggcg agcatgagcc cggccgacgt tctcctgcag
     2101 ccgatcttca acccggtggg cggctatgcg agttacgtcg tgccggcggc gttcatactg
     2161 atcctgcagc aaaccctgct gatcggcgcg gccatgctga ccagagcggc tctggcaaaa
     2221 ggcggcggcg cattcgccgg cgtgttcggc cgc
//
LOCUS       DATLJT010001372        10524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1953542,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001372 DATLJT010000000
VERSION     DATLJT010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953542"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VM910_31410"
     CDS             complement(<1..608)
                     /locus_tag="VM910_31410"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HVI67050.1"
                     /translation="MNWLTNVVRPKIRNMLRRETPENLWIKCPDSGQLVFYKDVEANQ
                     FVIPGSNYHMRMGAVARLKSIFDNETWFDVALPEVAADPLRFRDEKRYADRVKDARAR
                     TGLNDAIKIGYGKLEGAAVVIAVQDFDFMGGSLGMAAGEAIVRGFELAVEKKAPFIVF
                     AASGGARMQEGILSLMQLPRTTVAAQMLREAKLPYIVVLTNPT"
     gene            complement(712..1548)
                     /gene="trpA"
                     /locus_tag="VM910_31415"
     CDS             complement(712..1548)
                     /gene="trpA"
                     /locus_tag="VM910_31415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HVI67051.1"
                     /translation="MTTRIDARFAQLKKERRSAFVTFLMAGDPDPVTSLAILKAMPSA
                     GADVIEIGMPFTDPMADGPSIQAAGLRALKAGMTLKKTLQMVRDFRRDDDTTPLVLMG
                     YYNPIYIYGVDRFLADAKSAGVDGLIIVDLPPEEDTELCLPALKAGLNFIRLATPTTD
                     DKRLPAVLANTSGFVYYVSITGITGAAAANAKAVADAVARIKRHTSLPVCAGFGIRTP
                     EAAQAIAERADGAVVGTALVDALRGSLDPDGRATVRTVNTVASLAASLAQGVKGARQA
                     AE"
     gene            complement(1545..2762)
                     /gene="trpB"
                     /locus_tag="VM910_31420"
     CDS             complement(1545..2762)
                     /gene="trpB"
                     /locus_tag="VM910_31420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HVI67052.1"
                     /translation="MTKALPNSFRSGPDEHGHFGIFGGRFVAETLMPLILDLETAYAA
                     AKADPAFQAEMNGHLRDYIGRPSPLYFAERLTEHLGGAKIYFKREELNHTGSHKVNNV
                     LGQIMLARRMGKKRIIAETGAGQHGVATATLCARFGLECVVYMGAVDVARQQPNVIRM
                     EMLGAKVLPVQSGTRTLKDAMNEALRDWVTNVHNTFYCIGTVAGPHPYPMMVRDFQSV
                     IGNETRKQMQEAEGRLPDSLVACIGGGSNAMGLFHPFLDDPSVEIFGVEAAGHGLTQL
                     HAASIAGGRPGVLHGNRTYLLMNDDGQIQDAHSISAGLDYPGIGPEHSWLHEIGRVKY
                     LSATDDEALAAFQLLSRLEGIIPALEPAHAIAKVMELAPARPKDHLMVINLSGRGDKD
                     VPQVGDILKAKKK"
     gene            complement(2759..3418)
                     /locus_tag="VM910_31425"
     CDS             complement(2759..3418)
                     /locus_tag="VM910_31425"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HVI67053.1"
                     /translation="MPLLVKICGLSTRETLEAALTASADMVGFVFFPPSPRHLSFEAA
                     RELAGVVKHRAIKVALTVDADDATLDGIIEALEPDILQLHGHETAARVAEIKQKFSRE
                     VMKALPVEGAADLASLPAYAAVADRILFDARPPEGATRPGGLGAAFDWHVLQNLDLRV
                     PFMVSGGLHAGNVAEAVRVTRAGGVDVSSGVERTPGVKDVEMIRAFIRAARASQELTV
                     R"
     gene            complement(3460..3849)
                     /locus_tag="VM910_31430"
     CDS             complement(3460..3849)
                     /locus_tag="VM910_31430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HVI67054.1"
                     /translation="MRKFLTTLIVILLGLILVVFAVANRHFVTVSLDPFNSANPSISV
                     SLPLFVLIILVAILGVVAGGCATWFRQRRWRRAARQHEADARMAKAQLAEFRAATPVR
                     GEPLRIPPASQAGLYGASGRDKQGAAL"
     gene            complement(3904..4203)
                     /locus_tag="VM910_31435"
     CDS             complement(3904..4203)
                     /locus_tag="VM910_31435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HVI67055.1"
                     /translation="MIKSELVQRIAEHNPHLYQRDVENIVNAILDEIVAALARGDRVE
                     LRGFGAFSVKHRPARAGRNPRTGAHVPVDQKSVPFFKTGKEMRERLNRDNPETST"
     gene            complement(4375..5355)
                     /gene="sppA"
                     /locus_tag="VM910_31440"
     CDS             complement(4375..5355)
                     /gene="sppA"
                     /locus_tag="VM910_31440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HVI67056.1"
                     /translation="MSLDSDVIVDRRRIRRKLTFWRVAAVAIAIAAIAVIGTLALPGG
                     RSTFTTSGSIARVSIDGLIRSDRDRVEALERLEKSQAAAVVVHINSPGGTTAGSEQLY
                     DSLIRLKAKKPLVVVVEGLAASGGYITAIAADHIVAQQSSLVGSIGVLFQFPNVTELL
                     KTVGVKVEEVKSSPLKAAPNGFEPTSPEARAALDALVKDSYAWFKGLVKERRGLDDEQ
                     LEKVADGRVFTGRQAVDLKLIDQLGDEKTAVAWLVEQKGVKKDLPVRDYKLSPRFGDL
                     TFLRTAVSLTLDTLGLGAIAHQVEQAGMAQAVDRLSLDGMLALWSPAVGN"
     gene            complement(5547..7262)
                     /gene="rpsA"
                     /locus_tag="VM910_31445"
     CDS             complement(5547..7262)
                     /gene="rpsA"
                     /locus_tag="VM910_31445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVI67057.1"
                     /translation="MASSTTANTYSPSRDDFAAMLDESFAGGNLQESSVIKGKVVAIE
                     KDMAVIDVGLKTEGRVALREFSGPGRDSELKVGDEVEVFLDRIENALGEAVLSRDKAR
                     REESWGKLEKAFQNNEKVMGVIFNQVKGGFTVDLDGAVAFLPRSQVDIRPIRDVAPLM
                     NNSQPFQILKMDRRRGNIVVSRRTVLEETRAEQRQELVQNLEEGQVIDGVVKNITDYG
                     AFVDLGGIDGLLHVTDIAWRRVNHPTEVLTIGQTVKVKIIKINHETHRISLGMKQLLD
                     DPWQGIEAKYPLNARFHGRVTNITDYGAFVELEPGIEGLIHVSEMSWTKKNMHPGKIV
                     STSQEVEVQVLEVDSVKRRISLGLKQTMRNPWEVFVEKFPVGSVVEGEVKNKTEFGLF
                     LGLEGDVDGMVHLSDLDWKLPGEQVIDNYKKGDMVKAVVLDVDVEKERISLGIKQLEG
                     DPFAEPGDVKKGAVVTCEVLEVKDAGIEVKIVGTDFTTFIKRSELARDRNDQRSERFA
                     VGEKVDARVIQFDKKARKVQVSIKALEVAEEKEAIAQYGSSDSGATLGDILGTALKNR
                     GGGDK"
     gene            complement(7508..8146)
                     /gene="cmk"
                     /locus_tag="VM910_31450"
     CDS             complement(7508..8146)
                     /gene="cmk"
                     /locus_tag="VM910_31450"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVI67058.1"
                     /translation="MIIAIDGPAASGKGTLGKRLAKHYGYRHLDTGVIYRAVAYALIQ
                     AGADVNDEMLAVSAALELDPEKFGNPILKTQRVGEAASVVSAIPKVREVLLNFQRQFA
                     ADPPGAVLDGRDIGTVICPHADVKIFVMADPKVRARRRTLEARARGEDADEAAVLEDI
                     LNRDERDKNRAVAPLKPAADAYLLDNSNLDIEAGVRAAIDIVEAVRAGRQRA"
     gene            complement(8143..9480)
                     /gene="aroA"
                     /locus_tag="VM910_31455"
     CDS             complement(8143..9480)
                     /gene="aroA"
                     /locus_tag="VM910_31455"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HVI67059.1"
                     /translation="MTHSDHPSPLEARCSGPLTGKARVPGDKSISHRALILGALSVGK
                     TRISGLLEGEDVLNTAKSMRALGAEVDRTGDFAWVVQGVGVSGFAEPQNPLDFGNSGT
                     GCRLVMGAVAGCPITAVFDGDASLRSRPMRRVLDPLELMGAKTRSSGEGGRLPLTLAG
                     ARDPVPITYRTPVASAQIKSAVLLAGLSAPGVTTVIEQEASRDHTELMLKHFGAEIVS
                     SKEGAHGRKIALTGQPELHGADVVVPADPSSAAFPIVAALIVEGSDIILPDVMTNPLR
                     TGLFATLREMGALLEETEVRGDAGEPMAQIRARASKLRGVEVPPERAPSMIDEYLVLA
                     VAAAFAEGTTIMRGLHELRVKESDRLAATAAMLRVNGVSVEISGDDLVVEGRGHVPGG
                     GLVATHMDHRIAMSALVMGLASDKPVKVDDTAFIATSFPDFIPMMRALGAELS"
     gene            9626..10039
                     /locus_tag="VM910_31460"
     CDS             9626..10039
                     /locus_tag="VM910_31460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HVI67060.1"
                     /translation="MAKSDLGTKRICPTTGKKFYDLNKNPVISPYTGEVVPIAPVAPT
                     RGRAEAARAAPVPTAEAAPEPMEAEELVSPEEADAEENTGKVKAVVPESEEDIEIDET
                     IEDDDDDDSTFIADEEEGDEDVTDIIGDVSGDEET"
     gene            10162..10237
                     /locus_tag="VM910_31465"
     tRNA            10162..10237
                     /locus_tag="VM910_31465"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10195..10197,aa:Ala,seq:ggc)
     gene            complement(10252..>10524)
                     /locus_tag="VM910_31470"
     CDS             complement(10252..>10524)
                     /locus_tag="VM910_31470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67061.1"
                     /translation="TLTPLLKRLEAAHLIKRARSAEDERQVLISLTSQGHALKEKARA
                     LPQAILAASACSVAELSAMKNELVALRDRLNAVVGGHAWSARPRHA"
BASE COUNT         1946 a         3386 c         3251 g         1941 t
ORIGIN      
        1 gtcggattgg tcagcacaac gatgtagggc agtttcgcct cgcgcagcat ctgcgctgca
       61 accgtcgtgc gcggcagttg catcagcgac aggatgccct cctgcatgcg cgcgccgccg
      121 gaagcggcaa acacgatgaa cggtgctttc ttctcgaccg caagctcgaa cccgcgaacg
      181 atggcttcgc ctgcggccat gccgagcgag ccgcccatga aatcgaaatc ctgcacggcg
      241 ataacgacag ccgcaccttc gagcttgccg tatccgatct tgatcgcgtc gttgagcccg
      301 gtgcgggcgc gcgcatcctt gacgcggtcg gcatagcgct tctcgtcgcg gaatctcagg
      361 ggatcggcag cgacctccgg cagcgcgacg tcgaaccagg tctcattgtc gaagatcgac
      421 ttcagccgcg ctactgcgcc catgcgcatg tggtagttcg agccgggaat cacgaactgg
      481 ttggcctcga cgtccttgta aaacacgagc tgcccggaat cggggcactt gatccacagg
      541 ttctccggcg tctcgcggcg gagcatgtta cggatcttcg gccgaacgac attggtgagc
      601 caattcatgt ttcgctccga atcgcggtta tcgcccgctg gggatatatg gccggtctgg
      661 gattaaccgg caagtcgccg cccaaaacct taatctgcgg ggatttgcgg cctattccgc
      721 ggcctgcctg gcgcccttga cgccctgcgc cagcgacgcc gcaaggctgg cgaccgtatt
      781 caccgtcctg actgtggcgc ggccgtccgg atcgaggcta ccgcggaggg catcgaccag
      841 ggcggtgccg accaccgcgc cgtccgcgcg ctccgcgatc gcctgcgccg cttccggcgt
      901 cctgatgccg aaaccggcac agaccggcaa actggtgtgc cgcttgatgc gggcgaccgc
      961 gtccgcgacc gccttggcgt tggctgctgc ggcgccggtg atgccggtga tcgaaacgta
     1021 atagacaaag ccggaggtat tggcgagtac cgcaggcaag cgcttgtcgt cggtggtcgg
     1081 ggttgcgagc cggatgaagt tgaggccggc cttcagcgca ggcaggcaaa gttccgtatc
     1141 ctcctccggc ggcaggtcga cgattatcaa tccgtcgacg ccggccgatt tcgcatcggc
     1201 aaggaacctg tcgacgccat agatgtagat cgggttgtag tagcccatca gcacgagcgg
     1261 cgtcgtatcg tcgtccctgc ggaagtcgcg caccatctgc agcgtcttct tcaacgtcat
     1321 gccggccttc agcgcgcgca ggcccgcggc ctggatcgac ggaccgtccg ccatcggatc
     1381 ggtgaagggc atgccgatct cgatgacatc ggcgccggcc gaaggcatcg ccttcaaaat
     1441 ggcgagcgag gtcacgggat ccggatcacc cgccatcagg aaggtgacaa aagccgaacg
     1501 gcgctctttc ttcagttgcg caaagcgcgc gtcgatgcgg gtggtcactt cttcttcgcc
     1561 ttcaaaatat cgccgacttg cggcacgtcc ttatcgccgc ggccggagag attgatcacc
     1621 atgagatgat ccttcggccg cgccggcgcg agttccatca ctttcgcaat ggcatgcgcc
     1681 ggctccagcg ccgggatgat tccttcgagc cgcgagagca attgaaacgc tgcaagggcc
     1741 tcgtcgtcgg tcgccgataa atatttgacg cgcccgatct cgtgcagcca ggaatgttcc
     1801 ggcccgatcc cgggataatc gaggcccgcc gaaatcgaat gcgcgtcctg gatctggccg
     1861 tcgtcattca tcaagagata ggtgcggttg ccatgcagca cgcccgggcg cccgccggca
     1921 atcgacgccg catgcaattg cgtcagcccg tggcccgccg cttccacgcc aaaaatctcg
     1981 accgagggat cgtcgaggaa gggatgaaac agccccatcg cgtttgaccc gccgccgatg
     2041 caggcgacca gcgaatccgg caggcgccct tccgcctcct gcatctgctt gcgcgtctcg
     2101 ttgccgatca cggactggaa atcgcgcacc atcatcggat agggatgcgg cccggcaacc
     2161 gtgccgatgc aatagaacgt gttgtgcacg ttggtgaccc agtcacgcag tgcttcgttc
     2221 atcgcatcct tcagcgtgcg cgtgcccgac tgcaccggca acactttcgc gcccagcatc
     2281 tccatgcgga tgacattggg ctgctgccgc gcgacgtcga cggcgcccat atagaccacg
     2341 cattcgagac caaacctcgc acaaagcgtc gcggtggcaa cgccgtgctg gcccgcaccg
     2401 gtctcggcga tgatgcgctt cttgcccatg cgccgcgcca gcatgatctg gccgagcacg
     2461 ttgttcacct tgtgggagcc ggtgtggttc agctcctcgc gcttgaaata gatcttcgcg
     2521 ccgccgagat gctcggtcag acgttccgcg aaatagagcg gcgagggccg gccgatatag
     2581 tcgcgcaaat ggccgttcat ttctgcctgg aacgccggat cggccttggc ggcggcgtag
     2641 gctgtttcca ggtcgaggat cagcggcatc agggtttccg cgacaaagcg tccgccgaaa
     2701 atgccgaaat gcccgtgctc gtcggggccg ctgcggaagg aattgggcaa agcttttgtc
     2761 atcggacggt caactcttgc gatgcgcgcg ccgcgcggat gaaggcgcgg atcatctcga
     2821 cgtccttgac gcccggcgtg cgctcgacgc cggaggagac atcgacaccg ccggcgcgcg
     2881 tcacgcgaac ggcttccgcg acattgccgg cgtgaaggcc gccggaaacc atgaacggca
     2941 ccctgagatc cagattttgc agcacatgcc aatcgaacgc cgcgccaagg ccgccgggac
     3001 gggtcgctcc ctcgggcgga cgggcgtcga acaggatgcg gtcggcgacg gcggcatagg
     3061 ccggcaagct tgcgagatcg gcggcgcctt cgaccggcag cgccttcatc acctcgcggg
     3121 agaatttttg cttgatctcg gcaacccgcg ccgccgtctc gtgaccatgc aactgcagga
     3181 tatccggctc gagcgcctcg ataatgccgt cgagcgtcgc gtcgtcggca tcgacggtca
     3241 gcgccacctt gatggcgcgg tgcttgacca cgcctgcgag ctcgcgagcc gcttcaaacg
     3301 aaaggtgcct gggcgagggc ggaaagaaca cgaagccaac catgtcggcg ctagccgtaa
     3361 gcgccgcctc aagcgtctcg cgcgtggaca ggccgcagat tttgacgagc agtggcatgg
     3421 tctcttcggg ccgccaaagc gtgactgacg gggcgggttc tacaacgccg cgccctgctt
     3481 gtctcgcccc gaagccccgt aaaggcccgc ttgcgaggcg ggcggaatcc gcagcggctc
     3541 gccgcgtacc ggcgttgcgg cacgaaactc cgccaattgg gccttggcca tccgggcatc
     3601 cgcctcgtgc tgccgggccg cccgacgcca gcggcgctgc cggaaccagg ttgcgcagcc
     3661 gcccgccaca acgccgagga tcgcgaccag gatgatcagc acgaacagcg gcagcgagac
     3721 ggaaatcgag ggattggccg aattgaacgg atcgagcgat accgtcacga agtgacggtt
     3781 ggccaccgcg aatacgacga ggatcagccc cagcagaatg acgatcaggg tcgtgagaaa
     3841 cttgcgcatc acggggctcg tgttggatcg atcgcgaacg cccctggtct ctttcgagac
     3901 cccttaggtg ctcgtctcgg ggttgtcgcg gttcagccgc tcgcgcattt ccttgcccgt
     3961 cttgaaaaac gggacgcttt tctgatcgac cggcacgtgg gcaccggtgc gcggattgcg
     4021 cccggcccgc gcaggccgat gtttcaccga aaacgcgcca aacccacgca actcaacacg
     4081 gtcgccgcgt gccagagcgg cgacaatctc atcgagaatg gcgttcacga tgttctcgac
     4141 atcccgctgg tagagatgcg ggttgtgctc ggcgatacgc tgaaccagtt cggatttgat
     4201 catcgagagg ttggatccgg aagcgtcact tatccatttc cgtgaaaata ccttgatctg
     4261 tcaagacgct aaatcaattt tgcgcagcgc gaaatggcgt gacaaacgcc ccaatcgggg
     4321 cttgtcacaa tacccgcagc gcgggagaag cggtcaaaat cggctcagcc ccgttcagtt
     4381 gccgaccgcc ggactccaca gtgccagcat tccatcgagc gagaggcgat cgaccgcttg
     4441 cgccataccc gcctgctcga cctgatgcgc gatcgcgccg agtccgagcg tatcaagcgt
     4501 cagggatacc gcggtccgca ggaaagtcag gtctccgaaa cgtgggctaa gcttgtagtc
     4561 gcgcaccggc aaatccttct tgacaccctt ctgttcgacc agccaggcga ccgccgtttt
     4621 ctcgtcgcca agctggtcga tgagcttgag atcgaccgcc tgacggccgg tgaagaccct
     4681 gccgtcggcg actttttcaa gctgctcgtc gtccaggccg cggcgctcct ttacgagccc
     4741 cttgaaccac gcataggaat ccttcaccag cgcgtccagt gcggcgcgcg cctccggact
     4801 cgtcggctcg aagccgttgg gcgctgcctt cagcggcgaa gattttacct cctcgacttt
     4861 cacgccgacg gttttcagga gttcggtgac attcggaaac tggaacagca cgccaatcga
     4921 gcccaccagc gagctctgct gcgcaacgat atgatctgcc gcgatcgcag taatgtaacc
     4981 gccggacgcc gccaatcctt cgaccaccac gacgaggggc ttcttcgcct ttaaccgtat
     5041 cagcgaatcg tagagctgtt cggagccggc ggtggtgccg cccggcgaat tgatgtgaac
     5101 gacgaccgcc gcggcttgcg atttttccaa ccgctccaag gcctcgacgc gatcccggtc
     5161 gctgcggatc aagccatcga tgctgacccg cgcaatcgaa ccggatgtgg tgaacgtgct
     5221 gcgtccgcct ggtaatgcga gtgtgccgat gactgcgatc gctgcgatcg cgatcgccac
     5281 cgccgccacg cgccagaatg tcagcttgcg gcggatcctg cgccgatcga cgatcacatc
     5341 tgaatcaagc gacatcggaa tctcctcctg agggcagggt cggcgggttt tgaagatgcg
     5401 gcgcgagcat gcggttatct aacatcataa aattgcgacg caatatgctc aaacgaaggt
     5461 cctccgactc gcccgcgtga gcgagacgaa acaaaaggcc ccggctgtga ccggggcctt
     5521 cgtttgtcga gcaggtgagc aacgacttac ttgtcgccgc ccccgcgatt cttgagtgcg
     5581 gtgccgagga tgtcgcccaa ggtcgcgccc gagtcggaag agccgtactg ggcaatggcc
     5641 tccttctctt cggcgacttc cagcgccttg atcgacacct gcaccttgcg cgccttcttg
     5701 tcgaactgga tcacccgcgc atcgaccttc tcgccgaccg cgaagcgttc ggagcgctga
     5761 tcgttgcgat cgcgggcaag ctcggagcgc ttgatgaagg tggtgaagtc ggttccgacg
     5821 atcttcacct cgatgccggc gtccttcact tcgagcactt cgcaggtcac gaccgcgccc
     5881 ttcttgacgt cgcccggctc ggcgaaggga tcgccctcga gctgcttgat cccgagcgag
     5941 atccgctcct tctcgacatc gacgtcgagc accacggctt tcaccatgtc gcccttcttg
     6001 tagttgtcga tgacctgttc gcccggaagc ttccagtcga gatcggacag atgcaccatg
     6061 ccgtcgacat cgccctcgag gccgaggaac agaccgaact cggtcttgtt cttgacctcg
     6121 ccctcgacca ccgaaccaac cgggaatttc tcgacgaaga cttcccaggg gttgcgcatg
     6181 gtctgcttga ggccgagcga gatgcggcgc ttgacgctat ccacctcgag cacctgcact
     6241 tccacctctt gcgaggtcga aacgatcttg ccggggtgca tgttcttctt ggtccacgac
     6301 atctcggaga cgtggatcag gccctcgatg ccaggctcta gttcgacgaa cgcaccgtaa
     6361 tcggtgatgt tggtgacccg gccatggaag cgcgcgttca aggggtattt cgcctcgatg
     6421 ccctgccagg gatcgtcgag gagttgcttc atgccgagcg agatgcggtg cgtctcgtgg
     6481 ttgatcttga tgatttttac tttcacggtc tggccgatgg tcaagacctc ggtcggatga
     6541 ttgacccgcc gccaggcgat gtcggtgacg tgcaacaggc cgtcgatgcc gccgaggtcg
     6601 acgaacgcac cgtaatcggt gatgttcttg acgacgccgt cgattacctg accctcttcc
     6661 aggttctgca ccagttcctg gcgctgttcg gcacgggtct cttccagaac cgtgcggcgc
     6721 gacaccacga tgttgccgcg gcgccgatcc attttcagga tctggaacgg ctgcgaattg
     6781 ttcatcaggg gcgccacgtc gcggatcgga cgtatgtcca cttgcgaacg cggcaggaag
     6841 gctacggcgc cgtcgaggtc gacagtgaac ccgcccttga cctggttgaa gatgacgccc
     6901 atgacctttt cgttgttctg gaacgccttc tccagcttgc cccagctctc ctcgcgccgc
     6961 gccttgtcgc gcgacagcac ggcttcgccg agcgcatttt cgatgcgatc gaggaacacc
     7021 tcgacctcgt cgccgacttt cagttcgcta tcgcggcccg ggccagaaaa ttcgcgcaat
     7081 gcgacgcggc cctcggtctt caggccgacg tcgatgacgg ccatgtcctt ttcaattgca
     7141 accaccttgc ccttgataac ggagctttcc tgcaggttgc cgccggcgaa ggactcatcc
     7201 agcatcgcgg cgaaatcatc gcggctggga ctgtaagtat tagcagtggt actcgaagcc
     7261 atatgttctc caattgcgaa gtcttgccgg ccatttcggt tgagggcgta tcgcgcgtgc
     7321 agtgtcggtg gtcgcaaagc caatgacgac cgcttgttgc gctcatcgcg caaaagcggg
     7381 ccggcagatg cctgcgcgtt cgatacgttg agtttaggtc cggataggga tcggtcccga
     7441 aaaaccagtt tcgagatatc gactctcaag ggcgggagcg ggctcttcct ccaatgacgg
     7501 caggggctca agcccgctgc cggcccgctc ggacggcctc aacaatatcg atggcggccc
     7561 ggacgccggc ctctatatcc aaattggaat tatctagcaa gtaagcatcg gcggccggtt
     7621 ttaaaggcgc aaccgccctg ttcttgtcgc gctcgtcccg attgaggata tcctcaagaa
     7681 cagccgcctc gtcggcatcc tcgcctcggg cgcgcgcctc cagcgtccgg cggcgggcgc
     7741 gaaccttggg atcggccatc acgaaaatct tcacgtcggc atggggacag atcacggttc
     7801 cgatgtcgcg cccgtcgagc accgcgcccg gcggatcggc cgcaaactgc cgctggaaat
     7861 tgagcagcac ctcccgcacc ttcggaattg ccgagactac cgacgcggcc tcgccgaccc
     7921 tctgggtttt caggatcgga ttgccgaact tctcggggtc gagctcgagc gctgccgaga
     7981 ccgcgagcat ctcgtcgttc acgtcggcgc cggcctgaat cagggcataa gcgacggcgc
     8041 ggtagattac cccggtatcg aggtgacgat agccgtagtg cttggcaagg cgcttgccga
     8101 gggtgccctt gcccgacgca gccggtccgt cgatcgcaat aatcatgaaa gctcagcccc
     8161 cagcgcgcgc atcatcggaa tgaaatccgg aaaactggtg gctatgaagg cggtatcgtc
     8221 gaccttgacc ggcttgtcgg atgcaagtcc catcaccagt gccgacatcg cgatgcgatg
     8281 atccatgtgg gtggcgacaa ggccgccacc cggcacgtga cccctgccct cgacaacaag
     8341 atcgtcgccg gaaatctcga cgctgacacc attgacgcgc agcatggctg ccgtcgccgc
     8401 gagccggtcg gactccttga cccgcaattc gtgcagaccg cgcatgatcg tggttccttc
     8461 cgcaaaagca gcggcgaccg ccagcaccag atattcgtcg atcatggagg gcgcacgctc
     8521 cggcggcacc tcgacacccc gcagcttcga ggcgcgggcg cggatctgcg ccatcggttc
     8581 gccggcgtcg ccgcgaacct ccgtttcctc gagcagggcg cccatttcgc gcagcgtcgc
     8641 gaacaggccg gtgcgcagcg gattggtcat gacgtcgggc aggataatgt cggagccttc
     8701 gacgatcaga gccgccacga tcggaaacgc ggccgatgat ggatcggcgg gaacgacgac
     8761 atcggcgccg tgcagttccg gttgtccggt gagtgcgatc ttgcggccgt gagcgccctc
     8821 cttgctggag acgatttcgg cgccaaaatg cttcagcatc agttcggtgt gatcgcggct
     8881 cgcttcctgc tcgatcacgg tggtcacgcc cggcgcggaa aggcccgcga gcaggaccgc
     8941 ggacttgatc tgggccgacg ccaccggcgt gcgataggtg atcggcacgg gatcgcgcgc
     9001 accggcgagc gtcagcggca agcgcccacc ctccccgctg gagcgggttt tggcacccat
     9061 cagttccagg ggatccagca cccgccgcat cgggcgactg cgcagcgaag cgtcgccatc
     9121 gaaaactgcc gtgattgggc aacccgccac cgcccccatg accagtcggc agccggtacc
     9181 ggaattgccg aaatcgagcg gattctgcgg ctcggcgaac ccgcttaccc cgacgccctg
     9241 cacgacccag gcaaaatcgc cggttcggtc gacctcggcg cccagcgcgc gcatggattt
     9301 ggcggtgttc aaaacatcct cgccctcaag caggcccgaa atccgtgtct ttccgaccga
     9361 aagcgcaccc aaaatcaggg cccggtgcga aatcgacttg tcgccgggca cccgggcctt
     9421 gccggtcaga gggccggagc agcgtgcctc aagcgggctc ggatgatcgg aatgggtcaa
     9481 gaatgtgtcc taagctcctg cggcaggtaa cacagggctg gggcggcgtc acggccctgt
     9541 ctttcatgca cacagcgcta ttgacagctg gccctcaact agccaagtga agcaccgctt
     9601 tttcagacat tccaggattc cgcacgtggc caaatccgat ctcggaacca aacgcatttg
     9661 cccgaccacg ggcaagaagt tttacgatct gaacaagaat ccggtgatct cgccctatac
     9721 cggcgaggtc gtgccgatcg ctcctgttgc gccgacgcgc ggtcgtgccg aagccgcgcg
     9781 cgccgctccc gtgccgacag cggaagccgc gccagagccg atggaggccg aggagttggt
     9841 gtcgcccgag gaagccgacg ccgaggagaa taccggcaag gtaaaggctg tcgttcccga
     9901 gtccgaggaa gacatcgaga tcgacgagac catcgaagat gacgatgacg acgattcgac
     9961 cttcattgcc gacgaggaag aaggcgacga ggacgtcacc gacatcatcg gcgacgtcag
    10021 cggcgacgag gagacttgag atcgaccctg atctgtgatc agggtctgtt cggctggcgc
    10081 gtcacccaga cgggccggca ggcatgatcg ggaaaagcgg tcggccgttt ttccgaagag
    10141 gtcatgcgaa agcgaggtta cggggccata gctcagctgg gagagcgctt gcatggcatg
    10201 caagaggtcg gcggttcgat cccgcctggc tccaccacgc ttcgccttgg gctacgcgtg
    10261 gcgcggcctc gcagaccaag cgtgccctcc cacaaccgca ttcagccgat cgcgcaaggc
    10321 gaccagttcg tttttcatcg ccgacagctc ggcgacggag cacgccgagg ccgcgaggat
    10381 cgcctgcggc agggccctgg ccttctcctt cagtgcgtgg ccctgcgacg tcagcgagat
    10441 caggacctgg cgctcgtcct ctgcgctgcg cgcgcgcttg atcaaatgcg ccgcctcgag
    10501 gcgcttgagc agcggcgtga gcgt
//
LOCUS       DATLJT010001373         3258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2183174,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001373 DATLJT010000000
VERSION     DATLJT010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183174"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1250..1858)
                     /locus_tag="VM910_31475"
     CDS             complement(1250..1858)
                     /locus_tag="VM910_31475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67062.1"
                     /translation="MTPLEHCKNVTADRFQWTRIYLILKRASASRSSGEWNDDDFDVL
                     ADGAVVGRNLKVHAAPVGSPWIWTLAYGHHEDRTQTHGYAETSRGRDGDVRKELAANL
                     NARLVSLQVSDFTRMRVELTVRVSGLLIKGHYPASRALLPCRLISSTFGQALAVRIVA
                     NLAPCRNDYGKALVSVDTTPVGLLCAAADRRWLGGRGEIHPS"
     gene            complement(2358..2435)
                     /locus_tag="VM910_31480"
     tRNA            complement(2358..2435)
                     /locus_tag="VM910_31480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2398..2400),aa:Arg,seq:tct)
     gene            2702..>3258
                     /locus_tag="VM910_31485"
     CDS             2702..>3258
                     /locus_tag="VM910_31485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI67063.1"
                     /translation="MIAVRRLGHATLTTPDLDRQVAYYTAVVGLTLVERDGKRAFLAS
                     RQGLEAIALEAGPPNALSRLAFQVAPGSDLAEVARELAKHGIKSERRSCISPGVAEAV
                     VFKDPKDTWIEIFSEYVFAKEDLSQVGIMPLKLGHVAYRVRDVQQVVKFYTDVLGFRV
                     SDWRDETFAFLRCGPDHHTVNFVVD"
BASE COUNT          628 a          950 c          962 g          718 t
ORIGIN      
        1 gcgggagctg gtcatgctct cggcactggc tgcgcttggt tgtggtcttg cggcggtgca
       61 gggcgacctg ttgttcgtcg ctatctttgg cgtgacctgc gcagtgacaa ttatcgaagc
      121 gatacgacgt ttgcagtgag agcatcgggc gcgcggagtc ccgatccgag ccttgcctga
      181 tttgtgcttt ggaatagtat cggatgtcta gtgagactca ggtccctcta gggtttctcc
      241 tttgttagag aactcgaggc cccggcaagc ccggggcctt ctttaactca gcctcgcggc
      301 ttcttcggcg gcagcttgtg tgcgctgtca tctaagcatc atgttcgtct tcttattatg
      361 cgagcggcgg tgcttgttgg ggcttcgctg ctcttagttg ctttccgctg agactgagcg
      421 cagagtaaac ggatcgggac ttgccacctg tgcctagggc ggttgccgat gaccaattca
      481 gcaacagcac gaatcagtcc tcggccacgt ctgtgagccg atcgagcatt tcatgttcga
      541 tgaggatggg ttcgccaagg aggtgttcca tgccggtaag attatccctt accctcgtca
      601 tcttgatcgt ctgcactacg tggtacgccc gggctcagag cgtcccccaa aagaacacgg
      661 tccacttcca gaatggcatg tccgttgatg tcgctgtgtc gtcgggtgac cccacgagca
      721 agcaggaaag cttgatcggc aacgttcctg ccggaggcgc cccattagtt gggaacgctt
      781 tgcccgggac ggaattccgc gtcaggttcg ctcgacaagc cgcttggatc ggcacgtata
      841 taacgaccga tgctcctcag caatttgtga acatcgactg gggcaccctg ccagcccctg
      901 ggacagtccc gaaccggcct gcccaaccca tccccgcgag cagttttgca gggagctgca
      961 agaacatctg ggttaccgca gacgggctct atgcagactg ctaccgcgcc gacgggtcta
     1021 ctctcaaccg ctcgtcgatc ctgattaggg ggattagaaa tagaaactcg gaaggtaacc
     1081 tgtatttcgt tgggatggac gagccaagtt cgttccagta tacctgtagc gatatgaggg
     1141 tcggctccgg tggtaacaca ctggaggcgg actgcaggcg gtcggacggt cgctacaaca
     1201 aaacttcgat cctcatccct ggcatcgcga acaataacgg cgttctggtc tatgaaggat
     1261 ggatctcccc ccgacccccc aaccaacgcc gatccgctgc agcgcagagc aagccaacag
     1321 gtgtcgtgtc gacagagacc aatgccttgc cataatcatt tcggcaagga gccaagtttg
     1381 ccacgattct taccgccagt gcctgtccga atgttgagga aattaggcgg cacggcaaca
     1441 atgctcgcga ggctgggtaa tgtcctttaa tcaataggcc ggaaactcgc acggtgagtt
     1501 ccacgcgcat acgagtgaag tcgctgacct gaagactcac aagacgagcg tttaaattcg
     1561 ccgccagctc tttgcgaacg tcgccatcgc ggcctcgcga agtctcagca tagccgtggg
     1621 tttgcgtgcg atcctcatga tgtccatagg caagcgtcca tatccacggc gaccctaccg
     1681 gggccgcgtg taccttcaag ttacggccga ccacggcgcc atcggcaagc acgtcgaaat
     1741 cgtcgtcatt ccactcgccg gatgatcggc tcgctgaggc acgcttgagg atcagataaa
     1801 tccttgtcca ttgaaaacga tccgccgtga catttttgca atgttcgagc ggcgtcataa
     1861 ttggcgtaag cttggcgtaa gcaaatagtt ttaggatgag attctttgaa cttcaacctc
     1921 tgccccggtc gccactgccc actctcgcga ccggggcatt catttcgccg ggactttcga
     1981 cacgtctagc ttggcatcca tgaatagggc gagttcaagc gccctgctaa ccgtctgcac
     2041 atcggttttc gcgaggatca gcttgcagac ccgctgccag tggctacgca gctcccgctg
     2101 cacacgcatc gcggatggtg gtcagcgtgc cgccgtcttt ggtcgggaga ggctggtgta
     2161 gctttttgga catgtacgca ggacgcaaca tgacggaagt tagatagtct cgcgcattca
     2221 gaatgtcacc ttatgtcgcg tccatgttgc gtggagccgc cgtgagtcta tggcaaccat
     2281 tgaaaatgct tgtaattatt tgattgcaac atggacgcga catggtccac tcttctggcg
     2341 taagtggttg atttaattgg cgtccccggc aggattcgaa cctgcgacct gcggattaga
     2401 aatccgctgc tgctatccag ctgagctacg gggacgcgac ggggaagctt ttaccccaag
     2461 atcgccgtca tctgccagcg ccgcaacgac cgggttctgt cggaccgccc gcggctcggc
     2521 ggcatgggcg ggccgccggc gatgctcgca cttttctcct tccgctcttt ccgctcggtg
     2581 cgaaactggc gtaatttcgc atcgccatga gggcaagcat ccacgcgaga aaggctcgcg
     2641 ccgcgtcgcc tggcacgatc ttggctcgca cgcgccggtg ccggcgctcg ggaggcaagc
     2701 aatgatcgcg gtgcgcaggc tcggccatgc cacgctgacc acgcccgatc tcgatcggca
     2761 ggttgcctac tataccgccg tcgtgggcct caccctggtc gagcgcgacg gcaagcgcgc
     2821 ctttctcgcg agcaggcaag ggctggaggc gatcgcgctc gaggccggcc cgcccaatgc
     2881 gctgtcgcgg cttgcgtttc aggttgcgcc gggaagcgat cttgccgagg ttgcccgcga
     2941 gctggccaag cacggaatca agagcgagcg gcggagctgc atttctccag gagtcgccga
     3001 ggccgtcgtc ttcaaggatc cgaaggatac ctggatcgag attttctccg aatatgtctt
     3061 cgccaaggag gacttgagcc aagtcggcat catgccgctc aagctcggcc acgtggccta
     3121 tcgcgtgcgc gacgtgcagc aggtggtgaa gttctacacc gacgtgctcg gctttcgggt
     3181 atcggactgg cgcgacgaga ccttcgcgtt tctgcgctgc gggcccgatc atcacaccgt
     3241 caatttcgtg gtggacga
//
LOCUS       DATLJT010001374         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3331576,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001374 DATLJT010000000
VERSION     DATLJT010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331576"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VM910_31490"
     CDS             <1..896
                     /locus_tag="VM910_31490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HVI67064.1"
                     /translation="HVTGVVVNIMSMARCAGALIIMAEFKAAEYLKVAARERITYTVM
                     VPAMYNLCLLQPDFDSYDLSSWRIGGYGGAPMPVATIERLAAKVPSLRLMNAYGSTET
                     TSPSTLMPGDLTASYSDSVGLPCPGARIVVMDGEGREVPRGEIGEIWIGGAQVIKGYW
                     NNPKATAESFTGGFWHSGDLGSIDADNFVRVFDRQKDMINRGGLKIYSAEVESVLAAH
                     DSVVESAIIAKPCPVLGERVHAVVVTRDNVTAEVLRAWCAARLSDYKVPETMALTAEP
                     LPRNANGKVLKRLLRETLGAA"
     gene            1074..1727
                     /locus_tag="VM910_31495"
     CDS             1074..1727
                     /locus_tag="VM910_31495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI67065.1"
                     /translation="MSELFDEVDEEVRREQLKKLWERYSVFIVGAALLIIAAVGGWRG
                     YQYLEAQKAAEAGAAFNKAIELSEQNKRSEAEAAFADLVAKAPSGYRMLARFRLAAEI
                     ANRDPKAGAKMFDDLAADGSIGAEQQELARIRAAGVLLDSTSYPEMLRRLESETAPGA
                     TFRHVARELLALSAFRANDATATRQWLDQIALDSETPPGLRSRAEALQALLPPVAKS"
     gene            1753..2328
                     /locus_tag="VM910_31500"
     CDS             1753..2328
                     /locus_tag="VM910_31500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67066.1"
                     /translation="MRRPQRLIAAAVLIAFSGVLSGCSGWDPTDLLDFLDTKKKLPGD
                     RKPVFPDGVPGLEQGVPKDLYKGAQQQQQDQQATAVAPAQAAPPPDQPPAKQAKSKSK
                     SKQPAASDTAATQQPAEPDGEAAVASQENAAPSPKPKKVARKRTTAPPPDQSDAPAAQ
                     PAQTAQPAPQSAQQQSPPPFPAPLPSGTFTR"
     gene            2384..3754
                     /gene="der"
                     /locus_tag="VM910_31505"
     CDS             2384..3754
                     /gene="der"
                     /locus_tag="VM910_31505"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVI67067.1"
                     /translation="MSFTVAIIGRPNVGKSTLFNRLVGQKLALVDDEPGVTRDRREGA
                     ARLGDLDFTVIDTAGLDEGGRGSLTARMQEQTEAAIKLADALMFVVDARAGLTPTDRA
                     FADLARRAGKPVLLVANKSEGKHGDAGAMEAYALGLGDPIQISAEHGEGMGELYDALR
                     ALMPEPLEDAEASEADEDEDSSRPIRVAIVGRPNAGKSTLINHLVGEERLLTSPEAGT
                     TRDSIAVDIDWKGHFFRLFDTAGLRRRSRIEEKLEKLSVADALRAVRFAEVVVLLMDA
                     QNRFEEQDLRIADLIEREGRALVIAVNKWDLMPGKPGQISALRADADHWLPQVAGVPI
                     VAVSGLMGEGIDRLMQAILDAYAVWNKRVPTSALNRWFEQAIAANPPPAVSGRRLKLN
                     YITQTKARPPSFVLFCSRADAVPQSYLRYLTNSLREAFALPGTPVRITLREKQNPFAH
                     KRKRLS"
     gene            3751..>4584
                     /locus_tag="VM910_31510"
     CDS             3751..>4584
                     /locus_tag="VM910_31510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67068.1"
                     /translation="MNDGLPKPVTEASAPRSAAVAFIFVTILLDTLALGVIIPILPKL
                     IESFVSNDTASAARVFGLFGTAWALMQFIFSPVLGSLSDRFGRRPVVLLSNFGLSADY
                     VLMALAPSLAWLFIGRLISGITSASISTAFAYIADGTPPEKRAEVFGRIGVAFGAGFI
                     LGPALGGLLGDIAPRLPFWTAAGFSLANGLYGLFILPESLPRERRAAFRWKSANPIGM
                     LPLLRSSRTLAGLQAANFFAQLAHVVLPSTFVLYASYRYGWDAKTVGLTLAIVGICAM
                     IV"
BASE COUNT          794 a         1511 c         1481 g          798 t
ORIGIN      
        1 gccatgtgac cggcgtcgtc gtcaacatca tgagcatggc gcgctgcgcc ggcgcgctga
       61 tcatcatggc cgagttcaag gcggccgaat atctgaaggt cgcagcgcgc gagcgcatca
      121 cctacacggt gatggtgccg gccatgtaca atctctgcct gctgcagccg gatttcgaca
      181 gttacgatct gtcttcatgg cggatcggcg gctatggcgg tgcgccgatg ccggtcgcaa
      241 ccatcgaacg tctcgctgcc aaagtcccaa gcctgaggct gatgaacgcc tatggctcga
      301 ccgagacgac gtcgccctcg accctgatgc cgggcgatct gaccgcctct tacagcgaca
      361 gcgtcggact gccctgtccg ggcgcgcgca tcgtcgtgat ggatggagag ggccgcgaag
      421 tgccgcgcgg cgagatcggc gaaatctgga tcggcggcgc gcaggtcatc aagggctatt
      481 ggaacaatcc gaaggccacc gccgaaagct ttaccggggg cttctggcat tccggcgatc
      541 tcggctcgat cgatgccgac aattttgtgc gtgtgttcga ccgccagaag gacatgatca
      601 atcgcggcgg cctgaagatt tattcggccg aggtcgaatc cgtgctcgcc gcccacgaca
      661 gtgtggtcga gagcgcgatc atcgccaaac cctgcccggt actcggcgag cgcgttcacg
      721 ccgtagtggt gacgcgcgac aacgtcaccg ccgaggtgct gcgcgcctgg tgtgccgcaa
      781 ggctgtccga ctacaaggtt ccggagacaa tggctctgac cgcggagcct ttgccgcgca
      841 acgccaatgg caaggtgctg aagcggctgc tccgcgagac gcttggggct gcctgagggc
      901 ttgctccacg ttttccaccg tccgtccggt atgcccacca gagccttgat ccccgcttac
      961 tttgccttgc cagcgccata ccgataggct atcgcggccg ccatgctggc gctggatccg
     1021 gtccgcgcca ccttggcggc tgcccgccat tcctggacat tggatcctcc taagtgtctg
     1081 aattatttga cgaagttgac gaggaagtcc gccgggaaca gcttaaaaag ctgtgggaga
     1141 ggtattctgt cttcatcgtt ggcgcggcgc tgttgatcat cgcggcggtc ggcggctggc
     1201 gcggctatca atatctggag gcccagaaag ctgccgaggc gggcgccgcc ttcaacaagg
     1261 ccatcgaatt gtcggagcag aacaagcgca gcgaggcaga ggcggccttt gccgacctcg
     1321 tggcaaaggc gccgagcggc tatcgcatgc tggcgcgatt ccgtctcgcg gccgagatcg
     1381 ccaatcgcga tcccaaggcc ggtgcaaaaa tgttcgacga tctggcggct gatggctcga
     1441 tcggtgccga gcagcaggag ctggcacgga ttcgcgcggc cggcgtgctg cttgacagca
     1501 cgagctatcc cgaaatgctg cggcggctcg agtccgaaac cgcgccgggc gcaaccttcc
     1561 gccatgtcgc gcgcgagttg ctggcgctgt cggcgttccg cgccaacgat gccacggcca
     1621 cgcggcaatg gctggaccag attgcgctcg attccgaaac gccgccaggc ctgcgctcgc
     1681 gcgccgaggc gctgcaggcc ctgctgccgc cggttgccaa gagctagaga aagagctgac
     1741 cgagattacg atatgcgccg tccgcaacgt ttgatcgcag ccgccgtcct catcgccttc
     1801 tcgggcgtgc tgagcggttg ctcgggctgg gatccgaccg acctgctcga ctttctcgac
     1861 accaagaaga agctgccggg cgatcgcaag ccggtcttcc cggacggcgt gccgggcctg
     1921 gagcagggcg tgccgaagga cctctacaag ggggctcaac aacaacagca ggaccagcag
     1981 gctaccgccg ttgcgcctgc gcaggcagcc ccgccgcccg atcagccgcc ggcgaaacaa
     2041 gccaaatcca agtcgaagag caagcagccc gctgcatcgg acacggccgc tacccagcag
     2101 cctgccgagc cggacgggga ggccgccgtc gcatcgcaag agaacgcagc gccgtcgccc
     2161 aagccgaaga aggtcgcgcg caagcgcacc accgcgccac cgcccgatca gtcggacgcg
     2221 ccggcggccc agcccgcgca gaccgcccaa cccgcccccc aatcggcaca acagcaatcg
     2281 ccgccgccgt tcccggcgcc gctgccgagc ggcaccttta cgcgctaatt atctcccggc
     2341 acagcatttg acatgcgcaa accaagatgc gctggaatga tccatgtcgt tcaccgtcgc
     2401 catcatcggc cggcccaatg tcggcaaatc gacgctgttc aaccgcctgg tcggacagaa
     2461 gctcgcgctg gtcgatgacg aaccgggcgt gacgcgcgac cgccgggagg gtgcggcccg
     2521 gctcggcgat ctcgacttca ccgtgatcga taccgctggt ctcgacgagg gcgggagggg
     2581 atcgctgacg gcgcggatgc aggagcagac ggaagcggcg atcaagctcg ccgatgccct
     2641 gatgttcgtg gtcgacgcac gcgccggcct cacgcctacc gatcgcgcct ttgccgattt
     2701 ggcgcgccgg gccggcaaac ccgtgctgct cgtcgccaac aagagtgagg gcaagcatgg
     2761 cgacgcggga gcgatggagg cctacgcgct cgggctcggc gatcccatcc agatttcggc
     2821 ggagcatggc gagggcatgg gcgaactcta cgacgccttg cgtgcgctga tgccggagcc
     2881 gctggaagac gccgaagctt cagaggccga cgaggacgag gattcctcgc gccccatccg
     2941 cgtcgccatc gtcggccggc ccaacgccgg taaatcgact ctcatcaatc acctggtcgg
     3001 cgaggagcga ctcttgacca gtcctgaagc cggcacgacc cgcgactcga tcgcggtcga
     3061 catcgactgg aagggccatt tctttcgact gttcgatacc gcgggcctgc ggcgccgctc
     3121 gcggatcgag gagaagctgg agaaactctc agtcgcagac gctttgcgcg cggtgcgctt
     3181 tgcagaggtc gtcgtgctgc tgatggacgc gcagaacaga ttcgaggagc aggacctgcg
     3241 catcgccgac ctgatcgagc gcgagggacg cgcactcgtg atcgccgtca acaaatggga
     3301 tctgatgccg ggcaagcccg gacagatttc ggcgctgcgc gccgatgccg accactggct
     3361 gccgcaggtt gcgggcgtgc cgatcgtcgc cgtatccggc ctgatgggcg agggcatcga
     3421 ccgcctgatg caagcgatcc tcgacgccta cgccgtctgg aacaagcgcg tgccgacctc
     3481 agcactcaat cgctggttcg agcaggcgat cgcggccaat ccgccgcccg ccgtgtcggg
     3541 acgacggttg aagctgaact acatcaccca gaccaaggcg cggccgccga gctttgtgct
     3601 gttctgctcg cgtgcggatg cggtaccgca atcctacctg cgctatctca ccaactcgct
     3661 tcgcgaggcg tttgcgctgc cgggcacgcc ggtcaggatc acgttgcgcg agaaacaaaa
     3721 tccgttcgcg cacaagcgga aacgtttgtc gtgaacgatg gcctcccgaa gcctgtaacc
     3781 gaggcatcgg cgccccgtag cgctgcggtc gccttcatct ttgtcaccat cctgctcgat
     3841 acgctggcgc tcggcgtcat cattccgatc ctgccgaagc tgatcgagag ctttgtctca
     3901 aacgacaccg cgagcgccgc gcgcgtcttc ggcctgttcg gcacggcctg ggcgttgatg
     3961 caattcatct tctcgcccgt gctcggcagc ctgtccgacc ggttcgggcg gcggcctgtg
     4021 gttctgttgt cgaatttcgg gctctccgcg gactacgtgc tgatggcgct cgcgccatcg
     4081 ctggcctggc tcttcatcgg ccggctgatc tcgggtatca cctcggcaag catctcgact
     4141 gctttcgcct atatcgcgga cgggacgccg ccggagaaac gcgcggaagt gttcggcagg
     4201 attggggtgg cgttcggcgc cggtttcatc ctggggccgg cgctcggcgg cctgctcggc
     4261 gacatcgcac cgcggctgcc attctggact gcggcgggct ttagcctcgc caacggactc
     4321 tatggcctct tcatcttgcc ggaatcgctg ccaagggaac gccgtgcggc gttccgctgg
     4381 aaaagcgcca acccgatcgg catgttgccg ctgttgcgct cgagccggac gctcgcggga
     4441 ctgcaggccg cgaatttctt tgcccagctc gcgcatgtcg tgctgccgtc caccttcgtg
     4501 ctgtacgcgt cctatcgcta tggctgggac gccaagacgg tgggactgac gctcgcgatc
     4561 gtcggcatct gcgcgatgat cgtg
//
LOCUS       DATLJT010001375         5630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3792204,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001375 DATLJT010000000
VERSION     DATLJT010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792204"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VM910_31515"
     CDS             complement(<1..818)
                     /locus_tag="VM910_31515"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVI67069.1"
                     /translation="MMTLAIHDQRLAKPGPLNAALRDYDSVRRAIAFISEHWRTQPTI
                     EAIADSAALTPDELHHLFRRWAGLTPKAFMQALTLDHAKALLRDSASVLDAALDSGLS
                     GPGRLHDLFVTHEAMSPGEWKHGGNGMMLRYGFHPSPFGTAIVMATSRGLAGLAFADP
                     GEEQAALADMKGRWPNATYVEDYLSTAPLAQRIFVPKLWRSDQPLRVVLIGTDFEVRV
                     WETLLKIPMGKAVSYSDIANKISAPKASRAVGAAVGKNPVSFVVPCHRALGKDG"
     gene            complement(873..1394)
                     /locus_tag="VM910_31520"
     CDS             complement(873..1394)
                     /locus_tag="VM910_31520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HVI67070.1"
                     /translation="MTRGNDFDRDAAEPVLFSALLTPHRSLNRTGFLLVMAFLSVISF
                     AAGLVFLLMGAWPVLGFFGLDVLAIYWAFKINFRRARACEEIWVTPSELRLRRVNHRG
                     HRTEWVLNPLWVRLETVAHEEFGIEKLYLVSRGQRVSIGSFLGPDEKASFSNALMTAL
                     HAARRGPTYNPLA"
     gene            1417..2220
                     /gene="nth"
                     /locus_tag="VM910_31525"
     CDS             1417..2220
                     /gene="nth"
                     /locus_tag="VM910_31525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVI67071.1"
                     /translation="MAKITRAQRASKAVVRSLPKKSAKAKSARTAKTAANAKPKASKS
                     PKAKSPKRQLLKPWTPEEVREAFSRFRKANPEPRGELEHLNPYTLLVAVVLSAQATDA
                     GVNKATRELFKVADTPQKMLALGEEKLRDYIRTIGLYRNKAKNVIALSEKLIKDFGGE
                     VPRTRAEIEELPGAGRKTANVVLNMAYGEHTLAVDTHVFRVANRTGLAPGKTPLEVEL
                     GLEKIVPPEFMLHAHHWLILHGRYTCLARKPRCEVCLINDLCRWPEKTV"
     gene            2348..3556
                     /locus_tag="VM910_31530"
     CDS             2348..3556
                     /locus_tag="VM910_31530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67072.1"
                     /translation="MRLQPALVLAFASLFVMPSPSRAQISDNVVKIGVLNDQSGLYAD
                     LGGPGSVTAARMAAEDAGGMVLGKPIEIMFADHQNKADVGVAIARRWFENESVDMAIG
                     FDNSSVALAVEQLAAEHNRIAIAGAVGSTAFTSKNCTRNEASWIYDSYALTTSLAKST
                     VAEGRDTWFFITVDYAFGHSLEADATAAVQAAGGKVLGSVRHPLNTSDFSSYLLQAQA
                     SGAKVVAFANGGGDMVNAVKQANEFGLTKKQSMVALLIFISDVHSMQLQAAQGLKFVT
                     AFYWDRDDDSRAWSKRFFDKQGRMPTMAQASIYSAVRHYIKAIAAAGTDEARAVMVKM
                     RELPVNDFYVKNGHVREDGRLVHDMLFAQVKTPAESGKPWDYYKVLNVIPGDQAFRPL
                     AEGGCPLVAR"
     gene            3856..4908
                     /locus_tag="VM910_31535"
     CDS             3856..4908
                     /locus_tag="VM910_31535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HVI67073.1"
                     /translation="MNHTAALVSLATTVPPHQFHQTQVLFAARGVLAHRFAEFETLSS
                     LFANTGIRHRYGVKPIEWYLEPRGWPERTSAFLEGAELLFLDAARKAIARADLSAADI
                     DAVVTVCSTGIATPTLEARVASQMGFRSDVSRIPVFGLGCAGGVSGLSIAAKLAQARP
                     GTNVLLVALELCTLAVRHDTLTKANIVAASLFGDGAAAVVLRAGDGGATRIEAAGEKL
                     WPDTLDVMGWNVDPQGFGVVFQRTIPDFISERFKPAVAEIVSQMDLSIDDIDRFICHP
                     GGTKVIMALEGALSLDQGTLDHERDIIADYGNMSAPTVLFVLERARAQGLPPRSLLTA
                     LGPGFTASCVALRHDA"
     gene            4905..5417
                     /locus_tag="VM910_31540"
     CDS             4905..5417
                     /locus_tag="VM910_31540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVI67074.1"
                     /translation="MTLASLILTLVTLERIAELMLARRNTKALLARGATEVGANHYPW
                     IVLVHAAWLAALWIFGRNQQVSLAALAIYIALQGCRIWILSTLGSRWTTRIIVLPGEP
                     LIASGPYRYLSHPNYVIVVAEIAVLPLALHLPLLALVFTLLNGAVLVVRIRTEAQALS
                     ACTERVSRAS"
     gene            5420..>5630
                     /locus_tag="VM910_31545"
     CDS             5420..>5630
                     /locus_tag="VM910_31545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67075.1"
                     /translation="MLQEQPADVRRIAPSLPPSFATWTAFLLMCLGMFMAILDIQVVA
                     TSLPTIQDALGISPEAMSWIQTAYLI"
BASE COUNT         1061 a         1768 c         1753 g         1048 t
ORIGIN      
        1 ttgccgtcct tgccgagcgc gcgatggcag ggcaccacga acgataccgg gttcttgccg
       61 actgcagctc ccaccgcgcg cgacgctttc ggcgcgctga tcttgttggc aatgtccgaa
      121 taggaaaccg ccttgcccat cgggatcttc aggagcgtct cccacacccg aacctcgaaa
      181 tcggtgccga tcagcacgac gcgcagcggc tgatccgacc gccacagctt tggtacgaaa
      241 atgcgctgcg ccagaggcgc cgtgctcagg taatcctcga cataggtcgc attcggccag
      301 cgtcccttca tgtccgccag tgcggcttgc tcctcgcccg gatcggcgaa ggcgagccca
      361 gcgagcccgc ggctcgtcgc catgacgatc gcggtgccga acggtgaggg atgaaagccg
      421 taacgcagca tcatgccgtt gccgccatgt ttccattcgc cgggcgacat cgcttcatgc
      481 gtcacgaaca gatcatgcaa ccggcccgga ccggagaggc cggagtccag tgcagcgtcg
      541 agcacgcttg cggaatcgcg gagtagagcc tttgcatggt cgagcgtgag cgcctgcatg
      601 aaggcctttg gcgtcaatcc ggcccagcgg cgaaacagat ggtggagttc gtccggcgtg
      661 agggcagccg aatcggcgat cgcctcgatg gtcggttgcg tccgccaatg ttcggagatg
      721 aaggcgatcg cgcggcgcac tgagtcgtag tcgcgcaacg cggcgttcag gggacccggc
      781 ttggccaggc gctggtcgtg aatggcaagt gtcatcatgc cttcgatgta ggacgctacc
      841 gcgggcgaaa ccacccgatt tccgattatc cctcaagcca gcggattata ggtcggaccg
      901 cgccgtgcgg cgtgcagcgc ggtcattaag gcgttggaaa aacttgcttt ttcgtcgggc
      961 cccaaaaagc tgccgatcga caccctttgc cctctcgata ccagataaag cttctcgata
     1021 ccaaattcct cgtgggcgac cgtctcgagc cgcacccaga gcggattgag tacccattcg
     1081 gttctatggc cgcggtggtt gacccggcgc aggcgcagtt ccgacggcgt cacccaaatt
     1141 tcctcgcagg ctcgcgcacg acggaaattg atcttgaagg cccaatagat cgcgagcacg
     1201 tcgagcccga aaaagcccaa cacgggccag gcgcccatca gcaggaacac caaccccgcg
     1261 gcgaagctga tgacgctcag aaacgccatc accagcagga agccggtccg gttgagcgaa
     1321 cggtgcggcg tcagaagcgc tgagaacagc accggctcgg cggcatctcg atcaaaatca
     1381 ttgcctcgcg tcatcgcgta tcagtatatc cggatcatgg cgaaaatcac ccgcgctcaa
     1441 cgcgccagca aagctgtcgt tcgatcgttg ccgaaaaaat cggcaaaggc caagtcggca
     1501 cgcaccgcaa aaacggcagc aaatgcgaag ccgaaggcgt ccaaatcgcc caaagcgaaa
     1561 tccccaaaac gccaactcct gaaaccttgg acgcctgaag aggttcgtga ggccttcagc
     1621 cgttttcgca aggccaatcc ggagcccaga ggtgaactgg aacacctcaa tccctatacg
     1681 cttctggtcg ccgtcgtgct gtcggcgcag gcaacggatg ccggtgtaaa caaggcgacg
     1741 cgcgaattgt tcaaggtcgc cgacacgccg cagaaaatgc tcgcgcttgg cgaagaaaaa
     1801 ttgcgcgatt acatcaggac gatcgggctc tatcgcaaca aggcaaaaaa cgtcatcgcg
     1861 ctgtcggaaa agctgatcaa ggatttcggc ggcgaggtgc cgcgcacgcg cgccgaaatc
     1921 gaagagcttc cgggcgcggg acgcaagacc gcgaatgtcg tgctcaacat ggcctatggc
     1981 gagcacactt tggcggtcga cacgcatgtg tttcgcgtcg ccaatcgtac gggcctggcg
     2041 ccgggcaaga cgccgctcga agtcgagctt ggccttgaga agatcgtacc gccggagttc
     2101 atgctgcacg cacatcactg gttgatcctg cacggccgct acacctgcct cgcccgaaag
     2161 ccgcgctgcg aggtttgcct gatcaacgat ctgtgccgct ggccggagaa gacggtctga
     2221 attgggcgcg cccatcgatg cggcgcggtg ccccggatag ccgcgcttgt gggcaagcga
     2281 cggcgggcat aggctcttcc ccaaggtccg ccaaggatcg caaaagaatc tgcccgggag
     2341 gtgcgtcgtg cgattgcagc ctgcgctggt tttggccttc gccagccttt tcgttatgcc
     2401 ttcgccctct cgcgcacaaa tttccgacaa tgtcgtgaag atcggcgtgc tcaacgatca
     2461 atccgggctg tacgccgatc tcggcggccc aggctcggtc acagcggcgc ggatggcggc
     2521 cgaggacgcc ggcggtatgg tgctcggcaa gccgatcgag atcatgttcg ccgatcacca
     2581 gaacaaggct gacgtcggag ttgcgattgc gcggcgctgg ttcgagaacg aaagcgtcga
     2641 catggcgatc gggttcgaca attcgtccgt ggcgcttgcg gtcgaacaac ttgccgccga
     2701 gcataaccgc atcgcgatcg caggcgcggt cggcagcacc gccttcacca gcaagaactg
     2761 tacgcgcaac gaagcgtcct ggatttatga cagctacgcg ctgaccacca gcctcgccaa
     2821 gtcgaccgtt gcggagggtc gcgacacctg gttcttcatc acggtcgact atgccttcgg
     2881 ccattcgctg gaagcggatg caacggcggc ggtgcaggca gccggcggca aggtgctcgg
     2941 cagcgtgcgt catccgctca atacctcgga cttcagctcc tatttgctgc aggcccaggc
     3001 gtcgggcgca aaggtcgtgg cctttgccaa tggcggcggc gacatggtca atgcggtgaa
     3061 gcaagccaat gaattcgggc tcaccaaaaa gcagagcatg gtagcccttc tgatcttcat
     3121 ctccgacgtt cacagcatgc agcttcaggc ggcgcagggg ctgaaattcg tgaccgcctt
     3181 ctactgggat cgcgacgacg acagccgcgc ctggtcgaag cggttcttcg acaagcaggg
     3241 ccggatgccg accatggcgc aggcaagcat ctattcggcg gtccggcatt acatcaaggc
     3301 gatcgccgcg gccggtaccg acgaggcgag ggccgtcatg gtcaagatgc gcgagctgcc
     3361 ggtcaacgat ttctatgtca agaacgggca tgtccgcgag gacggcaggc ttgtgcacga
     3421 catgctgttc gcgcaggtga agacgccagc cgaatccggc aagccgtggg actactacaa
     3481 ggtcctgaac gtcattcccg gcgaccaggc gttccggccg cttgccgagg gcggctgtcc
     3541 cttggttgcg cgctgacctt cgcgacgccg ctgttgcggc caatcgtggc cacatttcaa
     3601 cttatagtga acggcttgag ccggctgttg tgatggtggc tgaaatattt gcttgcgtgc
     3661 gcggttgata ggccggggca ccggtgcggt ggttcggaag tcctcattct tgctgcacgt
     3721 tcggcaagat accctcccga atcggaccat atcgggatca cggatgacgt cctccgagcc
     3781 cgaagtttgc aacgagagcc cgccgcaaaa tctggcgaac tttggcaccg ggacgtgaga
     3841 ggcagggaca ggcacatgaa ccacacggcc gcgttggttt cgcttgcgac cacggttcca
     3901 ccccatcagt tccaccagac gcaggtcttg ttcgccgcgc gcggcgtcct cgcgcatcgc
     3961 tttgccgagt tcgagacgct gtcgagcctg tttgccaaca ccggcattcg gcatcgctac
     4021 ggcgtcaagc cgatcgagtg gtatctcgaa ccgcgcggct ggccggagcg gaccagtgcc
     4081 tttctggaag gcgcggaatt gctgttcctt gatgccgccc gcaaggcgat tgcgcgagct
     4141 gatctgtcgg ccgccgacat cgatgccgtg gtcacggtct gctccacggg cattgccacg
     4201 ccaacgcttg aagcacgcgt cgcaagccaa atgggttttc gatcggacgt gtcacgcatc
     4261 ccggtctttg ggctcggatg tgcgggcggc gtctcgggac tgtcgatcgc agccaagctc
     4321 gcgcaggcgc ggcccgggac caacgtgctg ctggtcgcgc tcgagttgtg cacgcttgcg
     4381 gttcgccacg ataccctgac caaggccaac atcgtcgccg ccagcctgtt cggcgatggc
     4441 gccgcggccg tcgtgttgcg cgcaggcgac ggcggcgcta cgcgaatcga agcggcaggc
     4501 gaaaagctct ggcccgatac gctcgatgtg atgggctgga atgtcgaccc gcaggggttc
     4561 ggcgtggtgt tccagcgaac gatccccgat ttcatcagcg aacgctttaa gccggcagtt
     4621 gccgaaatcg tttcgcagat ggatctttcg atcgacgaca tcgaccgttt catctgccat
     4681 cccggtggta cgaaggttat catggcactc gaaggcgctc tgtcactcga ccagggcacg
     4741 ctcgatcacg agcgcgacat catcgccgac tacggcaaca tgtcggcgcc gaccgtactg
     4801 ttcgtgctgg aacgcgcccg cgcgcaaggc ctgcccccgc gctcgctgtt gaccgcgctt
     4861 gggcccggct tcaccgcaag ttgcgtcgcg ctgcggcacg acgcgtgacg ctggcctcgc
     4921 tcattctgac cctggttacg ctggagcgta ttgccgagct catgctcgcc cgccgcaaca
     4981 ccaaggcgct gctggcgcgc ggcgcgacag aggtcggcgc caaccactac ccctggatcg
     5041 tgctggttca tgcggcctgg ctcgcagccc tttggatctt cggccggaat cagcaggtga
     5101 gccttgccgc ccttgcgatc tacatcgcgt tgcagggttg tcgtatctgg atcttgtcga
     5161 cactcggatc gcgctggacc acgcgcatca tcgtgctgcc gggcgagccg ctgatcgcga
     5221 gcggtcccta tcgatacctg tcccatccga actatgtcat tgtggtcgcc gagatcgcgg
     5281 tactcccgct cgcgctgcat ttgccgctgc tcgcgctggt gttcacgctt ctaaatgggg
     5341 cggttctcgt cgtccgcatc cgcaccgaag cccaggcgct gtcagcgtgc accgaacgcg
     5401 tgagcagggc gtcatgacga tgttgcagga acagcccgcg gacgtccggc ggatcgcgcc
     5461 atcgctgccg ccttcttttg cgacctggac cgcgttcctg ttgatgtgcc tcggcatgtt
     5521 catggcgatc ctcgacatcc aggtcgtggc gacgtcgctg ccgacgatcc aggatgcgct
     5581 cggcatttcg ccggaggcga tgagctggat ccagaccgcc tatctgatcg
//
LOCUS       DATLJT010001376         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2872852,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001376 DATLJT010000000
VERSION     DATLJT010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872852"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="pqqE"
                     /locus_tag="VM910_31550"
     CDS             <1..1119
                     /gene="pqqE"
                     /locus_tag="VM910_31550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HVI67076.1"
                     /translation="AFGIPLAVLAELTHRCPLQCPYCSNPIELDRARSELTTEEWKKV
                     LSELAELGVLQVHFSGGEPTARKDLVELVKHANDVGLYTNLITSAVLLTREKLAELAD
                     AGLSHVQISFQGSEAASADRVAGLKDAHNKKIEVAKWTRELDLPLTVNAVMHRQNLHQ
                     LPDIIQMAVDLDADRLEVANVQYYGWALKNRAALMPSLAQIEETSRLVEDARTRLKGI
                     LAIDYVVPDYYALRPKKCMGGWGRQFFNISPSGKILPCHAAESITGLQFESVRSNHSI
                     AWIWQNSEAFNRYRGTGWMPEPCRSCEFREVDFGGCRCQAFALTGDAGNTDPACTLSP
                     MHETIFKQAEREAASGSDRFIYRNFSGGTPEAGGDHGA"
     gene            1109..2092
                     /gene="pip"
                     /locus_tag="VM910_31555"
     CDS             1109..2092
                     /gene="pip"
                     /locus_tag="VM910_31555"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HVI67077.1"
                     /translation="MAPDADAGKPRRADPFQPLTSEQLAVGDGHEIYVESVGSFGGVP
                     AVYLHGGPGSGCQPDHRRLFDPERFHAVLFDQRGAGKSRPKGGRDQNTLAHLIADMEL
                     IREKFGFERWMVVGGSWGATLALAYTEAHPDRVSGLVLRAAFLGTYAEVEQAFCEALP
                     RFYPALSEDFLSVLPKEERSQPLQAYWRRILDSDPQVHTAPAHAWHDTERILSEHAPA
                     RTRLDLASLGSSRALPATPFMEAHYFSNDCFMAPDLLLEDAAKIAGIAGIVVQGRYDL
                     LCPPSTSQRLAARWPASEIRVIEGAGHSLYDPGIRDAVMRAIADMASRVTR"
     gene            2104..2790
                     /locus_tag="VM910_31560"
     CDS             2104..2790
                     /locus_tag="VM910_31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67078.1"
                     /translation="MPLAGKGMLLTSMDIDPADEADFNRWYDREHLEERVAIDGFLEA
                     RRYVAHTGSPKYLCLYSTTTIDVLDSPAYRARLENPTDWSRKTMARFKNMIRAVARIT
                     SSRGQGRGAMLGIVRLRPQGDENALRTTLKKRLDPATLDGIISMHLIESDPKLSGPTT
                     EISAAAGAGASDWFILIDGTNASAISAVLASRFTGTAAPEPALQISSGIYDLMWDLAK
                     SDIRSDVLPR"
     gene            3050..>3733
                     /locus_tag="VM910_31565"
     CDS             3050..>3733
                     /locus_tag="VM910_31565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HVI67079.1"
                     /translation="MKPTDISAPDYFHKVVDCQWACPAHTPVPEYIRLIAEGRYSDAY
                     MINWKSNVFPGILGRTCDRPCEPACRRGRVEETPVAICRLKRVAADFKDDIKHRLPRP
                     AAKNGKRIALVGGGPASLAVARDLAPLGYHCTVFDSDPKAGGMMRTQIPKFRLPDSII
                     DEETDYILNLGLEFKGGHRIDSMKKLLADGYDAIFVGSGAPRGRELDVPGRKEAAAHI
                     HIGIDWLSSV"
BASE COUNT          736 a         1184 c         1133 g          680 t
ORIGIN      
        1 gccttcggca ttccgcttgc ggtgctggcg gagctcacgc atcgctgccc gctgcaatgc
       61 ccctactgct cgaatccgat cgagctcgat cgcgcaagga gcgagctcac caccgaggaa
      121 tggaagaagg ttttgtccga gctcgccgag ctcggcgtgc tgcaggttca tttctccgga
      181 ggcgagccga ccgcgcgcaa ggacctcgtc gaactcgtca agcatgcaaa cgatgtcgga
      241 ctctatacca acctgatcac gtcggcggtg ctgctgacgc gcgaaaagct cgccgaactg
      301 gccgacgccg ggctttctca tgtgcagatc agctttcagg gcagcgaagc cgcgagcgcc
      361 gaccgcgttg caggcctcaa ggacgcgcac aacaagaaga tcgaggtcgc gaaatggacg
      421 cgcgagctcg atctgccgct gaccgtgaat gcggtgatgc accggcagaa cctgcatcaa
      481 ttgcctgata tcatccagat ggcggtcgat ctcgacgcag accggctgga agtcgccaat
      541 gtgcagtatt acggctgggc gttgaagaat cgcgccgcgc tgatgccgag ccttgcgcag
      601 atcgaggaga ccagccgcct ggtcgaagac gcgcggacgc ggctgaaggg cattctggca
      661 atcgattatg tggtgccgga ttactacgcg ctgcggccga agaagtgcat gggcggctgg
      721 ggccggcagt tcttcaacat ttcgccatcc ggaaagatcc tgccctgcca tgccgccgag
      781 agcatcacgg ggctgcaatt cgaatcggtg cggtcgaacc attcgatcgc ctggatctgg
      841 caaaactccg aggccttcaa ccgctatcgc ggcaccggct ggatgcctga accgtgcagg
      901 agctgcgagt ttcgcgaggt cgatttcggc ggctgccgct gccaggcctt tgcgctgacc
      961 ggcgatgccg gcaataccga tcccgcttgc acgctgtcgc cgatgcatga gacgattttc
     1021 aagcaggccg agcgcgaggc ggccagtggc agcgatcgtt tcatctatcg caatttttcc
     1081 ggcggcacgc cggaggcagg cggcgaccat ggcgcctgac gccgatgccg gcaaaccacg
     1141 gcgcgccgat ccatttcagc cgctgacatc ggagcagctc gcggtcggcg acggtcacga
     1201 aatatatgtc gagagcgtcg gcagcttcgg cggcgttccc gcggtctatt tgcatggcgg
     1261 gcccggctcc ggctgccagc cggatcatcg caggctgttc gatcccgagc gctttcacgc
     1321 ggtgctgttc gaccagcgcg gcgcgggcaa aagccgcccg aaaggtggcc gcgaccaaaa
     1381 cacgctggcc cacctgatcg cggatatgga gttgatccgc gaaaaattcg gctttgagcg
     1441 ctggatggtg gtcggcggct cctggggcgc gaccctggcg cttgcttata ccgaggcgca
     1501 cccggaccgc gtttcgggcc tcgtgctgcg cgcggcgttt ctcggcacct atgccgaagt
     1561 cgaacaggcg ttctgcgagg cgctgccgcg cttctaccct gcgctgtcgg aagattttct
     1621 gagcgtgttg ccgaaggagg agcgcagcca accgctgcaa gcctattggc ggcggattct
     1681 cgactccgat ccacaagtgc ataccgcgcc cgcgcacgcc tggcacgaca ccgagcgaat
     1741 cctctccgag cacgcgccgg cacgcacccg gcttgatctt gcctcgctcg gctcaagccg
     1801 ggcgctaccc gcaacgccgt tcatggaagc gcattatttt tccaacgatt gcttcatggc
     1861 accggacctg cttcttgaag acgcagcgaa gatcgccggc attgccggca tcgtcgtgca
     1921 gggccgctac gacctgttgt gtccgccctc gacatcgcag cgactggcgg cgcgctggcc
     1981 ggcttcggaa attcgtgtca tcgaaggcgc aggccattcg ctctacgatc ccggcatccg
     2041 cgatgcggtg atgcgggcga tcgccgacat ggcttcgagg gtgacgagat aaggatcgaa
     2101 aaaatgccgc tcgctggaaa aggcatgctg ctgacatcga tggatatcga tcccgcagac
     2161 gaagccgact tcaaccgctg gtatgaccgc gaacatctcg aagagcgggt ggcaatcgac
     2221 ggcttcctcg aggcacggcg ctacgtcgca cacaccggca gcccgaaata tctctgcctc
     2281 tactcgacca caaccatcga cgtgctggac agtccagctt atcgcgcaag gctcgaaaat
     2341 cccaccgact ggtccaggaa gaccatggcg cgtttcaaga acatgatccg cgccgtcgcc
     2401 cgcatcacgt caagccgcgg acaaggccgc ggcgcgatgc tcggcatcgt ccgccttcgg
     2461 ccgcaaggcg acgagaacgc tctccgcaca acgttgaaaa agcgactcga tcctgcaacg
     2521 ctcgacggca tcatctccat gcatctgatc gaaagcgatc ccaaattgtc gggcccgacc
     2581 acggaaattt ccgctgcggc gggtgccggt gcatccgact ggttcattct gatcgacggg
     2641 accaacgcga gcgcgatctc cgccgtgctc gcatcgcgtt tcactggaac tgcagctccc
     2701 gagcccgccc tgcagatttc atcgggcatt tacgatctga tgtgggacct cgccaaaagc
     2761 gacatcagaa gcgacgtctt accgcgctga accaatgcac gaattcggga tgcgccgcat
     2821 gttgcagtgc cgcaatagct aaaaacatta agttatggca ttgcaccgtt gaaaattagc
     2881 gatgcagcaa atttgtcttt gtgcagcagg ttgattggct ctaataacgt aaccctatga
     2941 tccaaatcaa aaaccataag gatgccgccc cccagaatcg gttatcgggc gagcgttcgc
     3001 tagcttaaag gccgcgcttt gcccagggcg tcagcgcggg aaggtaggaa tgaaaccgac
     3061 tgatatttcg gcccccgatt actttcacaa agtagtcgac tgccagtggg cctgtccagc
     3121 gcacacgccc gtcccggaat acatccgctt gatcgccgaa ggccgctaca gcgacgccta
     3181 catgatcaat tggaagtcga acgtgtttcc cggaattctg ggacgcacct gcgaccgtcc
     3241 ttgcgagccg gcctgccgcc gtggccgggt tgaggaaact ccggtcgcga tctgccgctt
     3301 gaagcgcgtc gccgccgact tcaaggacga catcaagcac cggctgccga ggccggccgc
     3361 aaagaacggc aaacgcatcg cgctggtcgg cggcggtcct gcgtcgctgg cggtcgcgcg
     3421 cgaccttgca ccgctcggct atcactgcac ggtgttcgat tccgatccga aagccggcgg
     3481 gatgatgcgc acgcagattc cgaaattccg cctgccggat tcgatcatcg acgaggagac
     3541 cgactacatc ctgaacctcg gcctcgagtt caagggcggt catcgcatcg acagcatgaa
     3601 gaagctgctc gcggacggtt acgatgccat cttcgtcggc tccggggcgc cgcgcggccg
     3661 cgagctcgac gttcccggcc gcaaggaggc cgccgcccat attcacatcg gcatcgactg
     3721 gctctcgagc gtg
//
LOCUS       DATLJT010001377         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_576815,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001377 DATLJT010000000
VERSION     DATLJT010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576815"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VM910_31570"
     CDS             complement(<1..459)
                     /locus_tag="VM910_31570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI67080.1"
                     /translation="MDLAFTKEELAFREEVRAFFRDNVPPETRRKQQEGRHLSKEEMI
                     TWWRILNKKGWGVSHWPKEYGGTGWSSVQHYIFNEELQMHPAPAPLAFGVSMVGPVIY
                     TFANEKQKKYYLPRIANVDDWWCQGFSEPGAGSDLASLKTKAERKGDKYII"
     gene            complement(625..>2290)
                     /gene="pimA"
                     /locus_tag="VM910_31575"
     CDS             complement(625..>2290)
                     /gene="pimA"
                     /locus_tag="VM910_31575"
                     /EC_number="6.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate--CoA ligase PimA"
                     /protein_id="HVI67081.1"
                     /translation="YPEGVRWDDPIARGTLPDLLSQAARDYSARTVIEFRDRAISYAE
                     LEGLVEAAAAAYLRAGYGKGTSVALFLGNTPDHPINFFGALKAGARIVHLSPLDGEIA
                     LTHKLRDSNARVLVTSNLAALLPTALKFLEKGLLDRLIVCEDDRWGKVGTPQTPLPDH
                     PAIITYAKFADGAVRPAQWPLIDVEDVALLQYTGGTTGLPKGAMLSHGNLTAAVSIYD
                     IWGKPSRARRGDAIERVICVLPLFHIFALTVVMLSSIRRGDLISLHQRFDVEAVMRDI
                     EVKRASSFPGVPTMWIAIAALPDLDKRDFSSLVSCGSGGAPLPVEVAKIFERRVGMKL
                     RSGWGMTETCSPGTAHPQEGPDRPGSIGLMLPGIEMDVVSLDDPAKVLSNGEVGEIRI
                     KGPNVTKGYWNKPRETAEAFVGDRFLTGDIGYRDDDGYFYLVDRKKDMIISGGFNVYP
                     QMIEQAIYTHPSVHEVIVIGIPDGYRGEAAKAFITLREGEKPFSLDELRAFLTGKVGK
                     HELPAEVEFVAELPRTAVGKLSRHELRRQQPSQPQQSKQRSTGSRS"
BASE COUNT          384 a          735 c          734 g          437 t
ORIGIN      
        1 gatgatgtat ttgtcgccct tccgctcggc cttggtcttc agcgaggcaa ggtcggagcc
       61 cgcacccggc tcggaaaagc cctggcacca ccagtcgtcg acattggcga tgcgcggcag
      121 gtaatatttc ttctgctttt cattggcgaa ggtgtagatg acgggaccga ccatgctgac
      181 gccgaaggcg agcggcgcag gcgccgggtg catctgcagc tcttcgttga agatatagtg
      241 ttgcaccgaa ctccaccccg tgccgccata ttccttcggc cagtgcgaga caccccagcc
      301 cttcttgttg aggatgcgcc accaggtgat catctcctcc ttgctgagat gacgcccctc
      361 ctgctgcttg cggcgcgttt cgggcggtac gttgtcgcgg aaaaacgcgc gaacctcttc
      421 gcgaaacgcc agctcttctt tcgtaaaagc gagatccatc ggatcctcct tgttgaaatt
      481 ccaaccgcct ctctcttcgt cgtcccggct cgcgctcgct aacgcttgcc tggccgggac
      541 gacagcttta gtgcaccacc tcgaacaaac ctgccgcgcc ctcgccgatc ggcgcgcggg
      601 cggtggagac gatgacagcg tcggtcatga acggcttcct gttgatcgtt gtttgctttg
      661 ttggggttgc gatggctgct gcctgcgcaa ttcatggcgc gacaatttgc cgaccgccgt
      721 gcgcggcaat tcggcgacga actcgacctc ggccggcaat tcatgctttc cgaccttgcc
      781 ggtcaggaag gcgcgcaact cgtcgagcga gaacggcttt tcgccctcgc gcagcgtgat
      841 gaaggccttg gccgcttcgc cgcggtaacc gtcgggaatg ccgatcacga tcacctcgtg
      901 cacggacggg tgggtgtaaa tcgcctgctc gatcatctgc ggatagacgt tgaagccgcc
      961 ggagatgatc atgtccttct tgcggtcgac caggtagaaa tagccgtcgt cgtcgcgata
     1021 gccgatgtcg ccggtgagga agcggtcgcc gacaaaggcc tccgctgtct cccttggctt
     1081 gttccagtag cccttggtga cattcggtcc cttgatgcgg atctcgccga cctcgccgtt
     1141 cgacagcact tttgccggat cgtcgagcga cacgacgtcc atctcgatgc cgggcagcat
     1201 cagtccgatc gaaccgggcc ggtccggtcc ctcctgcgga tgcgcggtgc cgggcgaaca
     1261 cgtctcggtc atgccccagc cgcttctgag cttcatgccg accctgcgtt cgaagatctt
     1321 ggccacttcg accggcagcg gcgcgccgcc cgagccgcag ctcacaagcg aggaaaaatc
     1381 gcgcttgtcg aggtccggga gtgcggcgat cgcgatccac atggtcggca cgccggggaa
     1441 ggacgaagcg cgcttgacct cgatgtcgcg catcacggcc tcgacatcga agcgctggtg
     1501 caacgagatc aggtcgccgc gccggatcga ggacagcatc acgaccgtca gcgcgaagat
     1561 gtggaacagc ggcagcacgc agatcacgcg ctcgatcgcg tcgccgcgcc tggcccggga
     1621 cggcttgccc cagatgtcgt agatcgatac cgccgcggtg agattgccgt gactcagcat
     1681 cgcgcccttg ggcaggcccg tggtgccgcc ggtatactgc aggagcgcga cgtcctcgac
     1741 gtcgatgaga ggccactgcg caggcctcac ggcgccgtcg gcgaattttg cgtaggtgat
     1801 gatggcggga tgatcgggca gcggcgtctg cggcgtgccg accttgcccc agcggtcgtc
     1861 ctcgcagacg atcagccggt cgagcagacc cttctcgagg aatttcaggg cggtcggcaa
     1921 caacgcggcg agattgctgg tgaccaagac gcgcgcgttc gaatccctga gcttgtgggt
     1981 cagcgcgatc tcgccgtcga gcggcgacag gtgcacgatc cgtgcgcccg ccttcagcgc
     2041 gccgaagaag ttgatcgggt ggtccggcgt gttgccgaga aacagcgcga ccgacgtgcc
     2101 cttgccgtag cccgcgcgca aatacgccgc ggctgcggct tcgaccaggc cttcgagctc
     2161 ggcataggag atcgcgcggt cgcggaattc gatgacggtg cgcgcgctgt agtcgcgcgc
     2221 cgcctgcgac aacaggtcgg gcagcgtgcc gcgcgcgatc ggatcgtccc agcgcacgcc
     2281 ttcggggtag
//
LOCUS       DATLJT010001378         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2873112,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001378 DATLJT010000000
VERSION     DATLJT010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873112"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..544
                     /locus_tag="VM910_31580"
     CDS             173..544
                     /locus_tag="VM910_31580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67082.1"
                     /translation="MSGETYLLEHINGKCVPKHCPVETQWHDGKCVALSAVSPSSEPE
                     PKSKPSAKETPKESSDEDEHRHCGRGMVRTRSGCVAIHHKLPGIYSGAPPGLLAAPAP
                     PNLTKYYRNYQNQTSKMPPPN"
     gene            complement(670..2007)
                     /gene="serS"
                     /locus_tag="VM910_31585"
     CDS             complement(670..2007)
                     /gene="serS"
                     /locus_tag="VM910_31585"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HVI67083.1"
                     /translation="MHDIKSIRENPQAFDAALQRRGLAPVSASLLAIDEKRRAAILAS
                     EQAQARRNAASKEIGEAKKAKDEPRAAALMAEVADLKTRMPELEQAAKAADEELSKEL
                     AAIPNLPLPAPEVPDGTDEHGNVLRHVFGKARDYGFTPKPHDDLGGTLGLMDFEAAAK
                     LSGARFVVLKKQLARLERAIGQFMLDLHTNEHGYAEINPPLLVRNDVMFGTGQLPKFE
                     DDQFWAVKGELLASPDERLRSERLGLIPTAEVSLTNLVRESIVDEKELPMRLTALTPC
                     FRAEAGAAGRDTRGMIRQHQFIKVELVSITTPETSKGEHERMLACAEEVLKQLDLHYR
                     VMTLCAGDMGFSAQKTYDIEVWMPGQGEGGAFREISSCSVCGDFQARRMDARYRAGDG
                     KPRFVHTLNGSGTAVGRALIAVMETYQQQGGSIAVPDVLQPYMGGLKVIEHTP"
     gene            complement(2111..>2407)
                     /locus_tag="VM910_31590"
     CDS             complement(2111..>2407)
                     /locus_tag="VM910_31590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVI67084.1"
                     /translation="AFGIAFQLPVILTLLGRVGIITSKMLREKRRYFIVIAFVIAAVL
                     TPPDVLSQSSLALPLLALYEGSIIAVSMVEKKAAAARASTTGTTGAPPAANPAE"
BASE COUNT          469 a          763 c          744 g          431 t
ORIGIN      
        1 ctgcgaggga ggtacggtga gcaacggctt atgcctgtgc ccagccggct tcaacgtcat
       61 gccgacgagc gccaatgcga atggggggac ctgcgtcagg accaacgcgg agaattgcca
      121 gggcggcgaa atgaccgtca acgggacttg cctctgcaac ggtcaagtcg tcatgtccgg
      181 cgagacctac ctgctcgaac acatcaacgg caaatgcgtc ccgaaacact gcccggtgga
      241 aacgcagtgg catgacggta aatgcgtggc gctgtcggcc gtctcgccca gttccgagcc
      301 tgaaccgaaa agcaaacctt cagccaagga aacgccaaag gaaagttcgg acgaggacga
      361 gcatcgccat tgcggtcgcg ggatggttcg cacccgctcc ggctgcgtcg ccatacacca
      421 caagcttcct gggatctact caggcgctcc gcccggtctc ctggcggcac ctgcaccgcc
      481 caacctcacc aagtattatc gaaactacca aaaccagacc agcaagatgc caccgccgaa
      541 ttgatgacgt tggaggtcac catcatcggc ggatacgcca catcggccgt tctcgccgcg
      601 cacggacgcg aacatagaga ggaccggtgt tcgcaaacat cgaagggagg aggaggcgcg
      661 agaaaaactc tatggcgtgt gctcgatcac tttgagtccg cccatatagg gctgcagcac
      721 gtcaggcacg gcaattgagc caccctgctg ctgataagtt tccatgacgg cgatgagcgc
      781 gcggccgact gccgtgcccg aaccgttcag cgtgtgcacg aaccgcggct tgccgtcgcc
      841 ggcacgatag cgggcatcca tgcggcgcgc ctgaaaatcg ccgcacaccg aacagctaga
      901 gatttccctg aaagcgccgc cctctccctg ccccggcatc cagacctcga tgtcataggt
      961 cttctgcgcg gaaaagccca tgtcgcctgc acataatgtc atcacccgat agtgcagatc
     1021 gagctgcttc aagacttcct cggcgcaggc cagcatccgc tcatgttcgc ccttgctggt
     1081 ttcgggcgtc gtgatcgaca ccagctcgac cttgatgaac tggtgttggc ggatcatgcc
     1141 gcgcgtgtcg cggcccgcag cccccgcctc ggctcgaaaa cacggcgtca gcgcggtgag
     1201 ccgcatcggt aattcctttt cgtcgacgat cgactcgcgg acgaggttgg tgagtgagac
     1261 ttccgccgtc ggaatcagac ccagacgctc gcttctcaga cgttcgtcgg ggctggccaa
     1321 caactcaccc ttgaccgccc agaactgatc atcctcgaat ttcggcagtt gtccggtccc
     1381 gaacatcaca tcgttgcgca cgagcagcgg aggattgatc tcagcatagc catgctcgtt
     1441 ggtgtgcagg tcaagcatga actggccgat tgcgcgttca agccgcgcca gttgcttctt
     1501 caacacgaca aagcgcgccc ccgacaactt tgcggcggcc tcgaaatcca tcaggccgag
     1561 cgtgccgccg agatcatcat gcggctttgg cgtgaagcca tagtcgcgcg ctttgccgaa
     1621 cacatgacgc agcacattgc cgtgctcgtc cgtgccgtcg gggacttcgg gcgcgggcag
     1681 cggcaaattg gggattgccg cgagttcttt cgaaagctcc tcgtcggcgg ccttcgcggc
     1741 ctgctcgagt tccggcatcc ttgtcttcag gtccgcgact tcggccatca gcgcggcggc
     1801 gcgtggctcg tccttcgcct tcttggcctc accgatctcc ttggaggccg cgttgcggcg
     1861 cgcctgcgcc tgctcggagg ccaaaatcgc cgcgcggcgc ttttcgtcga tcgcaagcag
     1921 cgacgcagac accggcgcaa gcccgcgccg ttgaagcgct gcgtcaaaag cctgggggtt
     1981 ctcgcggatc gatttgatgt catgcatggc ggatcgtcct gaatcgggcg ggcatccagt
     2041 aacccgaatg ggtgggattt ggaacagccg gggtgtgccg atattcggca gcgtcggggg
     2101 cgaaaggcgc ctactcggcc gggttggcgg ctggcggggc gccggtcgtg ccggtcgtgg
     2161 aggcgcgcgc cgcggccgcc ttcttttcca ccatgctcac cgcgatgatc gagccctcat
     2221 agagggcgag caacggcagg gccagcgagg attggctcag gacatccgga ggggtcagca
     2281 cagcagcgat gacgaaggca atgacgatga agtagcggcg cttctcgcgc agcatcttcg
     2341 acgtgatgat gccgacacgc ccgagcaggg tcaggatcac cggaagctgg aacgcgatgc
     2401 cgaaggc
//
LOCUS       DATLJT010001379         6420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2183694,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001379 DATLJT010000000
VERSION     DATLJT010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183694"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VM910_31595"
     CDS             <1..922
                     /locus_tag="VM910_31595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HVI67085.1"
                     /translation="PLIDKTVGEQLDAVGARIRTDPTLERAARVQWAKACRSMPLQGS
                     DATASLPPLWLELKPIRAFAAQPHVDATAVTLSVGIEAETRITPTETKPDCPFPEKIS
                     IVPPSSTGVSIGVPIDMPFTELNKILETQLSGRTFPEDGSGPVDVTVKHAYVAAAGDR
                     LLISLLVHAKEKKSFFGFGADATVHIWGHPVLDQTQQTLRLTDMQLAVESDAAFGLLG
                     AAARTVIPHLQRALTEKATVDLKPFAVNAQKKIADAIADFQKNDNGVRVEANISSIGL
                     SDIAFDSKTLRIIAEAAGAMTVTVTKLPEL"
     gene            1059..2276
                     /locus_tag="VM910_31600"
     CDS             1059..2276
                     /locus_tag="VM910_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67086.1"
                     /translation="MKQLATAAALGLLLAASSGSARAQAISDDVVKIGVLTDMSSLYA
                     DATGKGSLAAVQMAVEDYGGKVKGKPVEVISADHQNKPDVGVSIARNWYDNEKVDAIF
                     DVPTSSVALPISALTREKNRININSGGGSSDITGIACSPNTVHWTYDTYALSNVAGKA
                     MVKRGEDTWFFLTADYAFGMALERDAANVVKESGGKVIGDVRHPLNSSDFSSFLLQAQ
                     SSKAKVIALANAGGDTQNALKQAAEFGITQGGQKMIALLMQITDAHALGTKSAQGLIV
                     TDAFYWDMNDETRAFSKKFMDRVGHMPTMIQAGLYSATMHYLKAIEAIGTDEAPKVME
                     QMRAMPINDFFAKGGKIRIDGRMVHDMYLFEVKKPGESKGEWDLYKLLATVPGDEAFR
                     PLDKGGCPLVKTN"
     gene            complement(2326..2541)
                     /locus_tag="VM910_31605"
     CDS             complement(2326..2541)
                     /locus_tag="VM910_31605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HVI67087.1"
                     /translation="MSESVYKVIELIGTSKDSWEKAAATAVGRASKTLRDLRVAEVVK
                     LDMQLDETGAVEAYRAKLNVSFKFEGT"
     gene            complement(2742..3218)
                     /locus_tag="VM910_31610"
     CDS             complement(2742..3218)
                     /locus_tag="VM910_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVI67088.1"
                     /translation="MLAKYITTGVVGSLLLASAAFAEPPTVPQSQLHGDWRTSKVVGL
                     NVYNDNNENVGSINELLMDKSGNIKAAVLSVGGFLGMGARYVAVPFDKIKFSNEPVSY
                     AATSAKPNADAKPQSTTTTGSATVSAKPNPWYPDHAVFNANKDELKSMPEFKFSND"
     gene            complement(3359..3742)
                     /locus_tag="VM910_31615"
     CDS             complement(3359..3742)
                     /locus_tag="VM910_31615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67089.1"
                     /translation="MTLDQNPEIAADCRRQSVARHDLRIVVQLIHGATKMRLLRQQSC
                     LFGVQEKPRNVKQGEHEMTGANSTFLPKQNPCAQCGKPIAMPEWIETSPGRAAYLWHC
                     WDCDYRFEAIAYFDESTEQKAPLAA"
     gene            complement(3720..5213)
                     /locus_tag="VM910_31620"
     CDS             complement(3720..5213)
                     /locus_tag="VM910_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67090.1"
                     /translation="MTISVTHTDTAAQRMRGLLPLWVAVGVYTLFLLAGNRLLIDPDT
                     LWQITVGQWILDHHAVPETDIYSFTMRGQPWISTQWLAQVVYAKAYALAGWSGPVVVA
                     ATAIATTFALLTRFLTRRLSESTTLVFVAAALALTVPHLLARPHVLALPVMVAWVAGL
                     VAAADERAAPSWRLLPLMALWANLHGGFVFGLLLIAPIALDAVLAADEASRRVLIARS
                     AGFAVLALIASCCTPYGWNALLAAQKILSLGGALPLIMEWRPADFSSVTPFELCLLFG
                     IGLVLHRGITLPPMRIVLLLGLLHMALAQGRAAENLALVAPLILAAPLARQIGGSDVF
                     TSSTQPMRGALVACVATVLVAGTLAYTSVHRFAPHMHGAPVAAVAELKKHNLSRVFND
                     YDFGGYLIANGVAPFIDGRTELYGEKFFVDHNAASGLMEPDNLFRLLDAYNIEATLMR
                     TQSAATKLLDHVDGWQRIYSDDVATIHLRKAGALHTREPAVDAGPKS"
     regulatory      5410..5542
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5530..>6420
                     /locus_tag="VM910_31625"
     CDS             5530..>6420
                     /locus_tag="VM910_31625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI67091.1"
                     /translation="MYQNNVRSGGESPVSIIGAGIAGAWQALLFAQAGHDVTLHERGD
                     AAMTDSTSHWAGGMLAPYCESEVSEPVISRLGLRALDLWRRELPDTPFNGSLVVAHAR
                     DRSDFERFARLTSGHRRLDAAGLAALEPSLENRFREALFYPAEGHVEPRHVLPKLHER
                     IVAAGGTIRFNSDVSADALDGIVIDCRGLAARDTQNELRGVKGEMILIETDEVKLSRP
                     VRLIHPRWPLYVIPRDGNRFMLGATSIEAEDTGVSVRSALELLGAAYAVHPAFAEARI
                     VEFGSGLRPAFPDNLPRIAIE"
BASE COUNT         1241 a         2022 c         2057 g         1100 t
ORIGIN      
        1 gccgttgatc gacaaaaccg tcggcgagca gctcgatgcg gtgggcgcgc gaatccgcac
       61 cgatccgacg ctcgagcgtg ccgcgcgcgt gcaatgggca aaagcgtgcc gttcgatgcc
      121 gctgcagggc tcggacgcga ctgcctcgct gccgccgctg tggctcgagc tgaagccgat
      181 ccgggcgttt gccgcacagc cgcatgtcga tgcgaccgcg gtgacgctga gcgtcgggat
      241 cgaggccgag acacggatca cgccgacgga gaccaagccc gactgcccct tcccggaaaa
      301 gatctcgatc gtgccgccgt cgtcgaccgg agtgagcatc ggcgtgccga tcgacatgcc
      361 gttcaccgaa ctcaacaaga ttctcgagac gcagctttcg ggtcgaacct tcccggagga
      421 cggctcgggc ccggtcgatg tcaccgtcaa gcatgcctat gtcgcggcag ccggcgaccg
      481 gctcctgatc tcgcttctgg tgcacgccaa ggagaagaag agcttcttcg gattcggcgc
      541 cgatgccacg gtgcatatct ggggccatcc ggtgctcgat cagacccaac agacgctgcg
      601 cctgacggat atgcaactcg ccgtcgaatc ggacgccgcg ttcggactgc tgggcgcggc
      661 ggctcgcacg gtgataccgc atctgcagcg ggcgctgacc gagaaggcca cggtcgatct
      721 gaagccgttc gcggtcaacg cgcagaagaa gatcgcggat gcgatcgccg acttccagaa
      781 gaacgacaac ggcgtgcggg tcgaggccaa catctcgagc attgggctct cggatatcgc
      841 ctttgattcc aagacgctgc gcatcatcgc ggaagccgcc ggcgcaatga ccgtcaccgt
      901 gaccaaactg ccggaacttt agagccggac gtccccttca tcccatcctc cccgcctctt
      961 cgcggggcga aagaagcgcg ctttgccgcg gcacgcggat atggcaatac ccactgctca
     1021 agcgcagaca caacaagaga agcacaggga gaacaaccat gaagcaactt gctaccgcgg
     1081 ccgcgctcgg cctgctgctc gccgcgtcct ccggcagcgc gcgcgcccag gcgatctcgg
     1141 acgacgtcgt caagatcggc gtgctcaccg acatgtccag cctctatgcg gatgccaccg
     1201 gcaagggatc gctcgccgcc gtgcagatgg cggtcgagga ctacggcggc aaggtgaaag
     1261 gcaagccggt cgaggtgatc agcgccgatc accagaacaa gccggacgtc ggcgtctcca
     1321 tcgcccgcaa ttggtatgac aacgagaagg tggatgcgat cttcgacgtg ccgacgtcct
     1381 ccgtggcgct gccgatctcc gcgctgacgc gcgaaaagaa caggatcaac atcaattccg
     1441 gcggcggatc gtccgacatc accgggatcg cctgctcgcc gaacaccgtg cactggacct
     1501 acgacaccta tgcgctgtcg aacgtcgccg gcaaggcgat ggtcaagcgc ggcgaagaca
     1561 cctggttctt cctcacggcg gactatgcct tcggtatggc gctggagcgc gacgccgcca
     1621 acgtggtcaa ggaaagcggc ggcaaggtga tcggcgacgt ccgtcatccc ctgaactcgt
     1681 ccgacttttc ctcgttcctc ctgcaggcgc agtcctcgaa ggccaaggtg atcgcgctcg
     1741 ccaatgccgg cggtgatacc cagaacgcgt tgaagcaggc cgcagagttc ggcatcacgc
     1801 aaggcgggca gaagatgatc gcgctcctga tgcagattac cgacgcgcac gcgctgggca
     1861 ccaagtccgc gcaagggctg atcgtcaccg acgccttcta ttgggacatg aacgacgaga
     1921 cccgcgcctt ctcgaagaaa ttcatggatc gggtcggaca catgccgacc atgattcagg
     1981 ccggcctcta ctcggcgacc atgcattatc tgaaggcgat cgaggccatc ggcaccgacg
     2041 aggcgccgaa ggtgatggag cagatgcgcg ccatgccgat caacgacttc ttcgccaagg
     2101 gcggcaaaat ccgcatcgac gggcgcatgg tgcacgacat gtacctgttc gaggtcaaga
     2161 agcccggaga atccaaagga gagtgggacc tctacaagct gctcgccacc gtgcccggcg
     2221 acgaggcatt ccgtccgctc gacaagggcg gctgtccgct cgtgaagacg aattaggcga
     2281 atgagcggtg ggcacggcgc gggcgtccgt gcccaccctc ggagatcaag tgccctcgaa
     2341 cttgaacgag acgttcagtt tggcgcggta ggcttcgacc gctcccgtct cgtcgagctg
     2401 catgtcaagc ttgacgactt ccgcgacgcg aagatcgcgg agcgtcttgg aggcgcggcc
     2461 gaccgcggtt gcggcggctt tttcccagga gtccttactg gtgccgatta attcaataac
     2521 cttgtagacg ctttcgctca tgacgtcctc ccagttcggt tatgaagcag caggtagcag
     2581 ggcgctccct gctgccgcag cctaacatcg aagcgaccgg cgccgaaagg atcgcgcttg
     2641 ccttcgcggg cgaacaaaag cggaaaacga caacgcgccc ggcaggaccg ggcgcgttgg
     2701 ctacccccgc tatacggggc aggctccttc cggatgatgc gctagtcgtt ggagaatttg
     2761 aattccggca tcgacttcag ttcgtccttg ttcgcattga acacggcatg atccggatac
     2821 caggggttcg gctttgcgga cacggtcgcc gacccggtgg tcgtcgtcga ttgcggcttg
     2881 gcgtccgcat tcggcttcgc agaggtcgcg gcgtaagaga ccggttcgtt cgagaacttg
     2941 atcttgtcga agggcaccgc gacgtagcgt gcgcccatgc cgaggaagcc gccgacgctc
     3001 agcaccgccg ccttgatgtt tccgctcttg tccatcaaga gctcgttgat cgagccgaca
     3061 ttctcgttgt tgtcgttata gacgttgagg ccgacgacct tcgacgtgcg ccagtcgccg
     3121 tgcaactggg attgcggaac cgtcggcggt tccgcaaatg cggcgctcgc aagcaacagg
     3181 gaaccaacca cacctgtcgt gatgtatttc gccaacatga gttctctcca tcctcatcaa
     3241 actttaggtg gagaaaactt tcggaatgcg gacgcagttc cgtcacgccg cgcgttttcg
     3301 ttgcggcaaa gcgcgcaggt ttcaggaact cgcagacgac gtgtggcgtt gacgaagctt
     3361 aggccgcaag cggcgctttt tgctcggtgc tttcgtcgaa ataggcgatc gcctcgaagc
     3421 gatagtcgca gtcccagcag tgccagagat aggcggcacg gcccgggctg gtctcgatcc
     3481 attccggcat cgcgattggc ttaccacact gcgcacaggg attttgcttg ggcaaaaaag
     3541 tggagtttgc tccggtcatc tcgtgctctc cctgctttac gtttcttggt ttttcctgaa
     3601 ctccgaagag acaggattgt tgtcgcaaca aacgcatctt agtcgcgccg tgaatcaatt
     3661 gcacgacaat ccgcaagtcg tgtcgcgcga cactttggcg cctgcaatcc gcagcgattt
     3721 caggattttg gtccagcgtc aacggccggc tcgcgcgtgt gcagcgcacc tgccttgcgc
     3781 aaatggatgg tcgcgacgtc gtcggaatag atcctctgcc agccatcgac atgatcgagc
     3841 agcttggttg cggcgctttg cgtgcgcatc aaggtcgctt cgatattgta cgcgtcgagc
     3901 aggcggaaca aattgtccgg ttccatcaat ccggacgcgg cattgtgatc gacgaagaat
     3961 ttttcgccgt agagctcggt gcggccgtcg atgaacggcg cgacgccgtt tgcgatgaga
     4021 tagccgccga aatcgtaatc gttgaagaca cggctgagat tgtgcttctt cagttccgca
     4081 acggcggcga ccggcgcacc gtgcatgtgc ggcgcgaagc gatgcaccga ggtgtaggcg
     4141 agcgtgccgg cgaccagcac ggtcgcaacg caggcgacca gcgcgccgcg catcggctgc
     4201 gtgctcgagg taaagacatc gctgccgccg atctgccgcg ccagcggtgc ggcgaggatc
     4261 aggggagcga ccagcgccag attttccgca gcgcgtcctt gcgcgagcgc catgtgcaac
     4321 aggccgagca gcagcacgat gcgcatgggc ggcagcgtga tgccgcgatg aagcacaagg
     4381 ccgatgccga agagcaggca cagttcgaac ggcgtgacgc tcgagaaatc cgcgggcctc
     4441 cattccatga tcagcggcag cgcgccgccg aggctcagga tcttttgtgc ggcgagcaga
     4501 gcattccagc catagggcgt gcagcaggag gcaatcagcg cgagaaccgc aaagcctgcc
     4561 gagcgcgcga tcaggacgcg gcgcgacgct tcgtcggcgg ccaggacggc atcgagcgca
     4621 atcggcgcga tcagcagcaa gccgaacacg aaaccgccat gcaggttggc ccacagcgcc
     4681 atcaacggca aaagccgcca ggacggggcg gcccgctcat cggcggcagc gaccaggccc
     4741 gcgacccagg ccaccatgac cggcagcgcc aacacatgcg ggcgcgccag aaggtgcggc
     4801 acggtcagcg ccaacgccgc cgcgacgaac accagcgtcg tgctctcgct gaggcggcgg
     4861 gtcaggaacc tcgtgagcag cgcaaaagtc gtggcgattg cggttgcagc gaccacgacc
     4921 gggccgctcc atccggcgag cgcataggcc ttggcgtaaa ccacctgcgc cagccactgt
     4981 gtcgaaatcc agggctgacc gcgcatggtg aacgaataga tatcggtctc cggcaccgcg
     5041 tgatggtcga ggatccactg gccgaccgtg atctgccaga gcgtgtcggg gtcgatcagc
     5101 aggcgattcc cggcaagcaa aaacagcgtg tagacgccga ccgcgaccca aagcggcagc
     5161 agtccgcgca tcctttgggc ggcggtgtcg gtatgggtga ctgaaatcgt catcggatgc
     5221 gtgcgggacc agctcgggcc tcaatcgaag cacgccagga cataatttga ggtaaacccg
     5281 ctcgcccgtg ccatgcagca agcttttgta aagcaaggtt cttgccacgc tgccgacgag
     5341 ttccggcccg gcaggcagtg cagagctgcc ccgggcgcgt tgacaccctg cccgatcggc
     5401 ataaattagc gatgttccga ggggagctcc gaggaggagc tgagataccg caccctgctt
     5461 gccgcacgtg gcaagagtcc gcggtgaccc tttgaacctg atccgggtca tgccggcgaa
     5521 gggacaggga tgtaccagaa caacgtcagg tcagggggag aatctcccgt ttccatcatc
     5581 ggggcgggga tcgccggcgc atggcaggca ctgctgtttg cgcaggccgg gcacgatgtc
     5641 accctgcacg aacgcggcga cgccgcgatg acggattcca ccagccactg ggccggcggc
     5701 atgctcgcgc cctactgcga gagcgaagtg tccgagccgg tcatcagccg gctcggcctg
     5761 cgcgcactcg acttgtggcg gcgcgaattg ccggacacgc cattcaacgg ctcgctggtg
     5821 gtggcgcatg cgcgcgaccg cagtgatttc gagcgctttg cgcggctgac ctccggtcat
     5881 cggcggctgg acgcggcggg cctggccgcg ctcgagccgt cgctggaaaa ccgttttcgc
     5941 gaagcgctgt tctatcccgc cgagggccat gtcgagccgc gccacgtgct gcccaagctg
     6001 cacgagagga tcgtcgcagc cggcggcacc atccgcttca acagcgatgt ttccgccgat
     6061 gcgctcgacg gcatcgtgat cgactgccgg gggcttgcgg cgcgcgacac gcaaaacgag
     6121 ctgcgcggcg tcaagggcga gatgatcctg atcgagaccg atgaggtgaa actgtcccgc
     6181 ccggtacggc tgatccatcc gcgctggccg ctctacgtga ttccgcgcga tggcaaccgc
     6241 ttcatgctgg gagcgacctc gatcgaggcg gaggacaccg gcgtcagcgt ccgctcggcg
     6301 ctggagctgc tcggcgcggc ctatgcggtg catccggcct ttgccgaagc gcgcatcgtc
     6361 gaattcggct cgggcctgcg gcccgccttt cccgacaatc tgccgcgcat cgcgatcgag
//
LOCUS       DATLJT010001380         7537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2645704,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001380 DATLJT010000000
VERSION     DATLJT010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645704"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VM910_31630"
     CDS             complement(<1..437)
                     /locus_tag="VM910_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HVI67092.1"
                     /translation="MIAESGHYALVLALGLALIQSIVPLVGARWRDPALMNVARSTAL
                     AQLGFVAASFAALVTLHVTSDFSVANVFENSHSLKPLIYKITGVWGNHEGSMLLWVSI
                     LALFGALVAAFGNNLPLTLRARVLAVQAWVAGAFYLFILTTSNP"
     gene            complement(494..928)
                     /gene="ccmE"
                     /locus_tag="VM910_31635"
                     /pseudo
     CDS             complement(494..928)
                     /gene="ccmE"
                     /locus_tag="VM910_31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
     gene            complement(960..2069)
                     /gene="ccmI"
                     /locus_tag="VM910_31640"
     CDS             complement(960..2069)
                     /gene="ccmI"
                     /locus_tag="VM910_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HVI67093.1"
                     /translation="MTLWFVFALMTAAAIFAVLLPLGLGSRTRAGGDETNVYKDQLAE
                     IERDVAAGLIGPSEAEAARVEISRRLLAAADQEGGRPVKPNAKLRRAAAVLGLVGLPV
                     VAVAMYLPVGSPRLGDFPLSARARTPDGTQPLENLVAQVERHLEQNPNDGRGWTVLAP
                     VLARIGRFDDAVRAFRNSITYAGESADRRADLGEVMTAAAGGVVTAEAKAEFERALAL
                     NAEETKASYYLGLAAEQDGRNADAAAIWHAMLDKAPADAPWRPLVQEALTRVGGGGTE
                     APALSDKTISAAKNMSENDRTAMIRGMVDRLATRLKQNGEDVDGWLRLVRAYLVMGDE
                     EKARRAGADARQAVGSDAERLRLLNEGLKNLGFDG"
     gene            complement(2229..3641)
                     /locus_tag="VM910_31645"
     CDS             complement(2229..3641)
                     /locus_tag="VM910_31645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67094.1"
                     /translation="MGQTSYERLKNYLAQLPPQSQALLMRQFERAIERGEDTAVATLV
                     LEQLRKLARGTEKETPPRAEDQARLLFRPLEPFLVDGNVPVRPGQVRRSSLLPIWQWL
                     SRDGAPDQVREYQAALAQSSGEDDDPPLEAALRKLQFAAAEAIFKIAGPASRGDNQRA
                     LGRIGSPEVIEDLLPIGSVLQSREALEGLGGRLPMYLRMFGDSQVASLCASLNVPTLQ
                     TPQMLPFALSLIIQRLASPWQVIRIALKMAASDDELRVAATPYGLAVSLALHDLFCLA
                     AGLRLDIRRGQFANVSENLKALHDGVRGLRTELDLRHDSTWGKQLASIRADISNTLQS
                     EIDSVPGRVRRILRQRADKDIPAGARVDASEVEEVASLIDFVAVCRTCASELAINEVT
                     LRTYSDLQQYVEQATESLVQSLRAGDARLRNYRQMQAEAAIRFCDVLFGREYSTLMKR
                     AVENALTVERNAERKPSRAG"
     gene            4275..5186
                     /locus_tag="VM910_31650"
     CDS             4275..5186
                     /locus_tag="VM910_31650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67095.1"
                     /translation="MFTWILILGACAWVALLLLILHDVTKNDNEGSAILIAGNNVMTL
                     VRWAALKTGELFRWAVPRAGGVLRRTVLKARELRKAPGVRFSQATVSKPRVSLKAAVE
                     TGSLSLKPVRQDARPVIEQMLATPTPKIEQMPAASLPKIEQTPAASPPKIEQTLAAAP
                     TKIEQPLAALPPKVEQPPAAPPARKWWSKDTRVAKTAEELELTITEAVQATPGCEAFV
                     GVIIQRTTPKSRLDANWELRGTKFGRTDRKIAKEALTPIVERMQREFRLPERPTKSPF
                     ARSKAAADVRPITARGSVSASVHPPMN"
     gene            complement(5235..6614)
                     /locus_tag="VM910_31655"
     CDS             complement(5235..6614)
                     /locus_tag="VM910_31655"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVI67096.1"
                     /translation="MRRNSLATRLFLSASAWVVVILVITGFVLSSVYRSATERAFDRR
                     LNLYLRTLIAEVATPDEPPDRQFQSLGEPLFELPLSGWYWQITRTDSEKADVRASRSL
                     WDKKLPKLEDRGIELTAAGIRQAYVDGPEGQSLRMVERPVDLGADGKFLVSVAGDATE
                     IFDETRSFDYYLGGTFAALGIVLLLTTIFQVRFGLAPLKRISESIADIRSGRAERLEG
                     VFPVEIAPLARETNALIDANREIVERARTHVGNLAHAIKTPLSVIVNEASAHTADPFA
                     DKVLEQADVMRDQVAHHLERARIAARVTVVGTVTEVAPTIEALQRTMEKIHRHRGIVI
                     QLKADARARFRGERQDLEEMVGNLVDNACKWATSQVFIEVLVERALKPDADAMLRIIV
                     DDDGRGLSADERVQVSRRGQRLDESKPGSGLGLSIVVDLAALYGGSLSLGSAPIGGLR
                     AELVLPAAQ"
     gene            complement(6868..>7537)
                     /locus_tag="VM910_31660"
     CDS             complement(6868..>7537)
                     /locus_tag="VM910_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI67097.1"
                     /translation="RLLVVEDDPDLNRQLTTALSDAGYVVDRAFDGEEGHFLGDSEPY
                     DAVVLDIGLPKMDGISVLEAWRRNKRAMPVLILTARDRWSDKVQGFDAGADDYVAKPF
                     HLEEVLARIRALLRRSAGHAQSELTCGPVSLDTRTNRVSVSGNPVKMTSHEYRLLAYL
                     MHHAGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRIRKKLDVDIIQTVRGLGYLLTPP
                     PAGA"
BASE COUNT         1414 a         2437 c         2377 g         1309 t
ORIGIN      
        1 ggattcgagg tcgtgagaat gaagagatag aaggcgccgg cgacccaggc ctgcaccgcc
       61 agcacgcgcg cgcgcaacgt cagcggcaga ttgttgccga aggcagcgac gagcgcgccg
      121 aacaacgcca ggatcgagac ccacaacagc atcgagccct cgtgattgcc ccagacaccg
      181 gtgatcttgt agatcagcgg tttcagcgag tgcgagttct cgaacacgtt ggcgaccgaa
      241 aagtcggagg tgacgtgcaa cgtcaccagc gccgcgaagg acgcggccac gaagccgagc
      301 tgcgcgagcg cggtcgagcg tgcgacattc atcaaggcgg gatcgcgcca gcgcgcaccc
      361 accagcggca cgatggactg gatcaacgcc agcccgagcg ccagcaccaa tgcgtaatgt
      421 ccgctctcgg cgatcactgg gtcactctcc gtccctcgca ggtcgcgccg cccacattct
      481 gggcgagctt cttgccgtaa tcatccttcc agtggccctg cttcttgagc gcgtccgcaa
      541 cgtctcttgg catgtaggtc tcgtcgtgct tggcgagcac cgtatcggcc ttgaacacgc
      601 cggacccgtc gagcgcgcct tcggcaacca cgccctgccc ctcgcgaaac aggtcgggca
      661 ggcttccgga aaaactgacc ggcagtttgg cgctgccgtc ggcgacctcg aagcttacgg
      721 tcttgtcgcc gcatttgagc gagccgggct gcaccaggcc gccgagccgg aaccgcttgc
      781 cgggcgcgat gtgctgttcg gccgccatcg atggcgtcga gaagaacacg atcgacgatc
      841 gcaatccgta caggacgaga ccggccgcga gcgcgagcac cgcgagcgag ccgccgatca
      901 tcgtcaatct ccgctgcttg cgcgtcatgc ctgctcgctc tcgccgctgt cgatcccgct
      961 catccgtcga aaccgaggtt tttcaagcct tcgttgagaa gacgcagccg ttcggcgtcg
     1021 ctgccaaccg cctgccttgc gtcggcgccc gcacgcctgg ccttctcctc atcgcccatc
     1081 accagataag cgcgcaccag gcgcagccat ccatcgacgt cctcgccatt ctgtttcagt
     1141 cgcgtcgcaa gccgatcgac catgccgcgg atcatggcgg tgcggtcgtt ctcgctcatg
     1201 ttctttgccg ctgatatcgt cttgtccgat agtgccggcg cctcggtccc gccaccgccg
     1261 acccgcgtca acgcctcctg aaccagcggc cgccacggtg catcggcagg cgccttgtcg
     1321 agcatggcgt gccagatggc ggccgcatcg gcattgcggc cgtcctgctc ggctgcgagt
     1381 cccagatagt agctcgcctt tgtctcctcg gcgttgagcg ccagcgcacg ttcgaactcg
     1441 gccttggcct ccgcggtgac gaccccgccc gcggcggccg tcatgacctc accaagatcg
     1501 gcgcggcgat cggcactttc gccggcatag gtgatcgaat tgcgaaaagc ccgtaccgcg
     1561 tcatcgaagc gtccgatccg cgccagcacc ggtgccagca ccgtccagcc gcggccatca
     1621 ttcgggttct gctccagatg ccgctcgacc tgcgccacca ggttctcgag cggttgggtg
     1681 ccatccggcg tacgggcgcg cgcggacagc gggaaatcgc cgagccgtgg cgagcccacc
     1741 ggcagataca tcgcaaccgc aacaacgggc agaccgacga ggccgagcac cgccgccgcg
     1801 cgacgcagtt tcgcatttgg tttcacgggc cggccgccct cctgatcggc agccgccaac
     1861 agacgacgac tgatctcgac gcgcgcagcc tccgcttcgg agggtccgat caagcctgcc
     1921 gcgacatcgc gttcgatctc ggcgagctga tccttgtaga cattggtctc gtcgccgccc
     1981 gcccgcgtgc ggctgcccag ccccagcggc agcagcacgg cgaaaatcgc cgcggctgtc
     2041 atcagcgcaa agacgaacca caacgtcatc aggcgagttc cggcgatgca gacaaaaggg
     2101 ctccgatgcg cgctttacac tacccccgcc aattgcggca attgacaatt atcaattcgc
     2161 cgtaagctga ggtttgctga aaaggccagc cacaatagtc cgttagccgt ccgtcgggag
     2221 gtgttactct agccggcgcg ggatggcttg cgttccgcgt tccgttcgac cgtcagtgca
     2281 ttctcgactg cccgcttcat cagagtggaa tactcgcgac cgaacaggac gtcgcagaag
     2341 cggatcgccg cttctgcctg catttgccgg tagttgcgga gcctggcgtc gccggcgcgc
     2401 agcgattgca ccagcgattc ggtggcttgc tcgacatatt gctgcagatc cgaataggtc
     2461 cgcagcgtga cctcattgat ggcaagctcg ctggcacagg tgcgacacac cgcgacgaag
     2521 tcgatcagcg aggcgacctc ttcgacttcg gaagcatcca ccctggctcc cgccggaata
     2581 tccttgtccg cgcgctggcg caggattcgc cggacgcggc cggggacgct gtcgatctcg
     2641 gattgcagcg tgttggagat gtcggccctg atcgaggcca gttgcttgcc ccacgttgaa
     2701 tcgtggcgca ggtcgagttc ggtccgcagg ccgcgaacgc catcgtgaag cgccttcaaa
     2761 ttctcgctga cattggcgaa ctggccgcga cggatgtcca gtcgcagccc cgccgcgagg
     2821 cagaacagat cgtgcagcgc gaggctcacg gcaaggccat agggggtcgc cgccacccga
     2881 agttcgtcgt cggatgcggc catcttgaga gcgatgcgga tcacctgcca gggcgacgcc
     2941 aaccgttgaa tgatcagcga cagcgcgaac ggcaacatct gtggcgtctg cagcgtcggc
     3001 acattgagcg aggcgcaaag ggaggcgacc tgcgaatcgc cgaacatgcg caaatacatg
     3061 ggcagccggc cgccaagccc ttccagcgct tcgcgcgact gcagcaccga tccaatcggc
     3121 aacaggtcct cgatcacctc cggcgaaccg atgcgcccca gcgcacgctg gttgtcgccg
     3181 cgcgaggccg gccccgcgat cttgaaaatc gcctcggccg ccgcgaattg cagcttgcgg
     3241 agagcggcct cgagcggcgg atcgtcatcc tcgcccgagc tttgcgccag ggctgcctga
     3301 tattcgcgca cctggtcggg tgcgccgtcg cgcgagagcc actgccatat cggcaacagc
     3361 gagctgcggc gaacctggcc cggccgcacc ggaacgttgc cgtcgaccag aaacggctcg
     3421 agcggtcgga acaacagccg cgcctgatcc tcggcgcgcg gcggcgtctc cttttcggtt
     3481 ccccgcgcca gcttgcgcaa ttgctcgagc acgagagtgg ccacggcggt gtcttcgccg
     3541 cgctcgatag cgcgctcgaa ctgccgcatc agcagcgcct gcgactgcgg cgggagctgc
     3601 gcgagatagt tcttcagccg ctcgtaagat gtctggccca tgcgccctgc agaattgctc
     3661 gcgagacggc aaaagcgtcc accccgcacc ataagtgccc cggcgttaag aagtcgttat
     3721 ccgaaagata cggaaaatca gcggcttatg ctgcctggcg ggcagggttg cgacacctga
     3781 aacgattgag aaattttccc ggctgccgtg gcttcacttc gcgcgcgact acgccgtcgg
     3841 cgaactggcg catgcaccgt gtggcgttgg cggaaacaga tggagcgatg agacggcctt
     3901 aaggccaatc gagaacgcgt cgcgtctgct ccgatcgacc agcccgccgg ttccggatga
     3961 cgtgctgcgc gggccgtacg gccgcaatct gtagcgcgtt gcatcctgat ctgttgttcc
     4021 gataatcagc gcagccgctc gccggcgatc agtgcagatc gaaggccgga cgacgttcgg
     4081 ttgtccgata cagaggatca gtttacctgc acagcccgtg gcaagcgcgg cgcagacgtg
     4141 aggccgggcg gaataaaaat ttagtcgtcg taatcgcgtg tcgtttgcga gctccggatg
     4201 gcgaggccgt ctgacttctg ttacgattgg agaataaaaa agagcaagcc ttccgaaagt
     4261 gaaggagtac ggtgatgttc acctggattt tgatcctggg tgcttgcgcc tgggttgcgt
     4321 tgcttctgct gattttgcat gacgtgacca aaaacgacaa tgaaggcagc gccattttga
     4381 ttgccggcaa caatgttatg acgcttgtcc gttgggccgc gctgaagacc ggagaattgt
     4441 ttcgttgggc cgtgcccagg gccggagggg tgcttcgtcg gaccgtgctc aaggccagag
     4501 agttgcggaa agctccaggc gtcaggtttt cgcaggcgac cgtatcaaag ccacgcgtga
     4561 gtttgaaagc ggcagttgaa accggctctt tatccttaaa gccagtccga caagacgcgc
     4621 gacctgtgat cgagcagatg ctcgctacgc cgacaccgaa gatcgaacag atgcccgctg
     4681 cctcgctacc gaagatcgag cagacgcccg ctgcctcacc accgaagatc gagcagacgc
     4741 tcgctgcggc gccgacgaag atcgagcagc cgctcgctgc gctgccgccg aaggtcgagc
     4801 agccgcccgc cgcgccaccg gcgaggaagt ggtggagcaa ggacactcgg gttgcaaaaa
     4861 cagccgaaga gctcgaattg acgatcaccg aagcggttca ggctacgcct ggctgcgaag
     4921 ccttcgtggg tgtgattatc cagcgcacaa cgcccaaatc gcgtctggat gcaaattggg
     4981 agcttcgggg gacaaagttc ggccggacgg atcgaaaaat agccaaggaa gctttgacgc
     5041 ccatcgtcga gcgcatgcag cgggaatttc gtcttcccga acgtccgacc aagagcccgt
     5101 ttgcgcgatc caaggccgcc gcggatgtac gcccgatcac tgcaagaggc agtgtgagcg
     5161 cctccgtgca tccccccatg aactagggtc gtgaactaat cttcgggctg ttgccggcaa
     5221 cgccgcccga gacgctattg cgccgcaggc aggaccagtt cggctcgcag gcctccgatc
     5281 ggcgcactgc cgagcgacag gctgccgcca tagagggcgg caaggtcgac cacgatcgac
     5341 agcccgagcc cggagcccgg cttggattcg tccagccgct ggccacggcg ggagacctgt
     5401 acgcgctcgt cggccgacag gccgcgcccg tcatcgtcca cgatgatccg cagcatcgcg
     5461 tcggcgtcgg gcttgagcgc ccgctccacc agcacctcga tgaaaacctg cgaggttgcc
     5521 catttgcagg cgttgtcgac gagattgccg accatctcct ccagatcctg ccgctcgccg
     5581 cggaaccttg cccttgcatc ggccttgagc tgaatgacga tgccgcggtg gcggtgaatc
     5641 ttctccatgg tccgctgcag cgcctcgatt gtcggcgcga cttcggtcac cgtgccgacg
     5701 acggtgacgc gcgccgcgat gcgcgcgcgc tccaggtgat gcgcgacctg gtcgcgcatc
     5761 acgtcggcct gttccaaaac cttgtctgcg aaagggtcgg cggtgtgcgc gctggcctcg
     5821 ttgacgatca ccgacagcgg cgtcttgatg gcatgcgcga gattgccgac atgggtacgc
     5881 gcgcgctcga cgatctcgcg gttggcatcg atcagcgcgt tggtctcgcg cgcgagcggc
     5941 gcgatctcga cgggaaatac gccctccagc cgctcggcgc ggccggagcg tatatcggcg
     6001 atcgattccg aaatgcgctt cagcggtgca aggccgaagc ggacctggaa gatcgtggtc
     6061 aacagcagca cgatgccgag cgcagcaaag gtgccgccga ggtaatagtc gaagctgcgc
     6121 gtctcgtcga aaatctcggt cgcgtcgccc gcgacgctca ccagaaactt gccgtcggcg
     6181 ccgaggtcga ccgggcgctc gaccatccga aggctctgcc cctccggccc gtcgacatag
     6241 gcttgccgga tgccggccgc ggtcagctcg atgccgcggt cctcgagctt cggcagtttt
     6301 ttgtcccaca gcgagcggga agcgcgcaca tcggcctttt cggagtcggt gcgggtaatc
     6361 tgccagtacc agccggacag cggcagctcg aacaagggct cgccgagcga ctgaaactgg
     6421 cggtcgggcg gctcgtcggg ggtcgcgacc tcggcgatca gcgtgcgcaa atagaggttg
     6481 agccggcgat cgaaggcgcg ttccgtcgcg cttctgtaga ccgacgacag cacaaagccg
     6541 gtgatgacga ggatcaccac cacccaggcg gacgcggaca agaacagccg ggtggcaagc
     6601 gagttgcggc gcatgatgcc gctagtgtcc cggttctgac attcgtatca tgtcgcagca
     6661 atccctttta cgaatgtcag aaccaaaggg acacgaataa aaacagtgat gctcgtgcgg
     6721 ccttggattt gacattcgct tcgcgattag cgggcacgaa gcagcgaatg tcaaatctgc
     6781 cgcaacagcg ccaatttgaa gtccatgcag tttgcgaacg acaggtgccg cgggccgatc
     6841 gcgaacttcg gcgagcgggc actcgtatca ggcacccgcg ggcggcggcg tcagcaaata
     6901 ccccaatcca cggacggtct ggatgatgtc gacgtccagc ttcttgcgga tgcggccgac
     6961 aaacacctcg atggtgttgg agtcgcggtc aaaatcctgg tcgtaaagat gctcgacgag
     7021 ttcggtgcgc gacaccacgc gcccggcatg gtgcatgaga taggcgagca gccggtactc
     7081 gtgcgaggtc atcttcacgg gattgccgga gacgctgacg cggttggtgc gggtatcgag
     7141 cgacaccggg ccgcaagtca gctcggactg ggcatggccg gcggagcggc gcaaaagggc
     7201 gcgtatccgc gccagcactt cctcgaggtg aaagggcttt gcgacgtaat cgtcggcacc
     7261 ggcatcaaag ccctgaacct tgtcgctcca gcggtcgcgc gcggtgagga tcaaaacagg
     7321 catcgcgcgc ttgttgcggc gccaggcttc gagcaccgag atgccgtcca ttttcggcag
     7381 tccaatgtcg agcacgacgg catcatacgg ctcgctgtcg cccagaaagt gcccctcctc
     7441 gccatcgaac gcacgatcta ccacataacc ggcgtcgctc agcgctgtgg tgagctgacg
     7501 gtttaagtcc ggatcgtcct caacgaccag caggcgc
//
LOCUS       DATLJT010001381         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2645977,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001381 DATLJT010000000
VERSION     DATLJT010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645977"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VM910_31665"
     CDS             <1..315
                     /locus_tag="VM910_31665"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HVI67098.1"
                     /translation="LAVTCSPLAPGQATLSDPVLVIEILSPSNQAKTWTNVWAYTSIP
                     SVREILVLRADRIAAELLRRSPQGEWPDRPIAIAEGELALESIGFGMALAELYARTGL
                     GA"
     gene            350..712
                     /locus_tag="VM910_31670"
     CDS             350..712
                     /locus_tag="VM910_31670"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HVI67099.1"
                     /translation="MAGLIKQNYSPQSTQRLRQGPPTTYPRLVVKNGCCDARALTYVS
                     CDHKVTYMEKTQVYLPREELEALRAAAARSGRSVAELIREAIRKTVLKPRSAGPVALW
                     DGEPKRTSIEHDSVHDEP"
     gene            750..1106
                     /locus_tag="VM910_31675"
     CDS             750..1106
                     /locus_tag="VM910_31675"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVI67100.1"
                     /translation="MDRPCSFTRPLTCAGERACARLYTSVPVVIETFTFLDRNASRDV
                     ALAWQEAIYKPGTVAILPCEPRDLEQSWEYFRHTDLYKLSAVDATSFAIMKRERIRLA
                     YAFDHHFAVVGFRLVT"
     gene            complement(1319..1525)
                     /locus_tag="VM910_31680"
     CDS             complement(1319..1525)
                     /locus_tag="VM910_31680"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HVI67101.1"
                     /translation="MEWIDQSEAYGEKRWNLLGMCGGVILHVTYTERGERIRIISARR
                     AERHEQDRYSSSTSQSIMPAREAT"
     gene            complement(1664..2047)
                     /locus_tag="VM910_31685"
     CDS             complement(1664..2047)
                     /locus_tag="VM910_31685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HVI67102.1"
                     /translation="MTSYIGLIRKEASSDFGVDFPDFPGCVSAGATLDEARRMAQEVL
                     ELHVSGMVEDGEELPAPSSLEAIMADPENGDAVAFLVTLREPADRTVRVNITLPERLL
                     RRIDERAKNRSAFLARAAEKALAES"
     gene            complement(2058..2193)
                     /locus_tag="VM910_31690"
                     /pseudo
     CDS             complement(2058..2193)
                     /locus_tag="VM910_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530010.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
BASE COUNT          415 a          689 c          668 g          421 t
ORIGIN      
        1 ctggcggtca cttgctcgcc tttggcgccc gggcaggcca ccctctccga cccggtgctg
       61 gtgatcgaga tcctttcccc gagcaaccag gcgaagacct ggaccaatgt ctgggcgtac
      121 acgagcatcc cgagcgttcg ggaaatcctg gttctccgtg ccgatcggat cgcagcggag
      181 ctgctgcggc gttcgccgca gggagaatgg cccgatcgcc cgatcgcgat cgccgagggc
      241 gaactggcgc tggagagcat cgggtttggg atggccctag ccgagcttta tgcgcgcacc
      301 gggttggggg cctgactgaa aatcgggctc gtcatcgacc acgaggatct tggccgggct
      361 cataaaacaa aactattcac cgcaaagtac gcaaaggctg cgccaaggac caccaaccac
      421 ctatccccgc ttggtcgtga agaacggctg ctgcgacgcc cgggccttga catatgtcag
      481 ttgcgaccac aaagtgacat acatggaaaa aacccaggtt tacctccccc gggaagaact
      541 ggaggcgctg cgcgcggctg ccgcccggtc ggggcgcagc gtcgccgagc tgatccgcga
      601 ggcaatccgc aagacggtgc tcaagccgcg atccgcaggg ccggttgccc tctgggacgg
      661 agaaccaaag cgcacctcga tcgaacacga tagcgttcat gacgagcctt gatacggcga
      721 agtgaggcgg ttttcgtcga cagcggagcg tggatcgccc ttgctctttc acgagacccc
      781 ttacatgtgc cggcgagaga gcatgcgcga ggctctacac atcggtaccc gtcgtcatcg
      841 agacattcac atttctcgat cgcaacgcca gtcgggacgt ggcgctggct tggcaagagg
      901 caatctataa gccggggacc gtggcgattc ttccctgcga gccgcgcgat ctggagcagt
      961 cgtgggagta ctttcgacac accgacctat acaagctgtc ggcggtcgac gccaccagct
     1021 ttgcgatcat gaagcgcgag cgaattcggc ttgcctacgc gttcgatcat cactttgccg
     1081 tggtcggttt caggctggtg acatagcagt atcggctgac gcaacagtgg atggcgatga
     1141 agaggcgacg gcaagaggtg ccgccggggc gactgctatt gcggggccgg cgcgccgacg
     1201 agcgcatcaa gattgtccgg ttcggcgatc agctgataga acctcggtga gctcagcaag
     1261 aaacgtcctc gccagcgccc gacgccaacg atctgctcgc ccgcggggtc tttgacagct
     1321 acgttgcctc tctcgcgggc attatggatt gggacgtgct gctggaatat ctgtcttgct
     1381 catgtcgttc cgcgcgacgc gctgagataa ttctgatccg ttcaccgcgc tccgtgtagg
     1441 tcacgtgcag gatcacgccc ccgcacatgc ccagtaggtt ccaacgtttc tcgccgtaag
     1501 cctcgctctg gtcgatccat tcgatagcga acgccgtaga tcggccagcg cgtcgtcgag
     1561 catcgccatt cgcctatcct caccgtcacc ggcgacgata cccttcctcc ccctgcctgc
     1621 gagcagaggt gcgcaaaagg aatcggctcg cggccttttt cgctcagctc tccgcgagag
     1681 ccttctccgc ggcgcgagcc aagaacgccg agcggttttt ggcacgctca tcgatacggc
     1741 ggagcaggcg ctcaggcagg gttatattca cgcgcacggt gcggtctgcg ggctcccgca
     1801 aagtgacgag aaaggcgacc gcgtcgccgt tttcggggtc ggccataatc gcttccaggg
     1861 aactcggggc gggcaactct tcgccgtcct cgaccatacc gctgacatga agctcaagca
     1921 cttcctgggc catgcggcgc gcctcatcga gcgtcgcgcc ggcgcttaca cagcccggga
     1981 agtccgggaa gtctacgccg aaatcgctcg aagcttcctt tctgatcaga ccaatatagc
     2041 tcgtcattgg ataatgccta tctcgtgggc atgagccgga gaccggcttg gcgttcgatg
     2101 cttttcaccg tgcctaggca ccgtcacccg gcctttgcgg aacgggtgtt taagctgaac
     2161 gtggctgccc tttcgagcga cctcgaacca ccc
//
LOCUS       DATLJT010001382         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2874143,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001382 DATLJT010000000
VERSION     DATLJT010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874143"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VM910_31695"
     CDS             complement(<1..525)
                     /locus_tag="VM910_31695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI67103.1"
                     /translation="MTSTDAPARVAPLTAKRESSPVDVKSSTRSPKDQSRPFIKPLLK
                     SHRELKRPPFDCIALVLQGGGALGAFQAGVYQGLAEANLHPDWVAGISIGAINAALIA
                     GNAPEARLEKLRAFWEQVTTQLLFDPFGVAHYWLRGDTGHVFLNRLRAGATLLEGAPG
                     FFKPRIPPPYFAPPG"
     gene            complement(628..939)
                     /locus_tag="VM910_31700"
     CDS             complement(628..939)
                     /locus_tag="VM910_31700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67104.1"
                     /translation="MLWTVADKVPSLDVESSCRAAAKMGDSLSLDATLRQCLADEKSA
                     RDQLEKQWTQFPAALRQRCVTTTETGGDPSYVEVLVCLQMGRDAAQTEDPLGGGRQDQ
                     K"
     gene            complement(1208..1837)
                     /locus_tag="VM910_31705"
     CDS             complement(1208..1837)
                     /locus_tag="VM910_31705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67105.1"
                     /translation="MMRARIAVAKLAMNTLRYFLPPALLAVSVAAPASAQYACTTNYC
                     ISTQSISFQTPPGWPAVEVETCCRTHWGALPSGIDWSNGAPIMICVGRPEYVTSFPNC
                     GDPQNVSQAFPLDYERPTFAIGVAWPNAKDYRYEMQSVPLVDGPDGTCAQYSSQPGQV
                     TNWWVHELKEPKRRTYVFTAAVSFQKGEKGYYAVLNSCKLSPPSSPPVR"
     gene            1952..>2335
                     /locus_tag="VM910_31710"
     CDS             1952..>2335
                     /locus_tag="VM910_31710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVI67106.1"
                     /translation="MRCVPKDLLRAAQARLLLLAGMGMLTVLAACGQENRFIAPPPPK
                     VTVQLPLQQTFTPYLEATGNAASVNTVKLLARVEGYVQEIKYQDGAAVKKGTPLFVIE
                     PEPYKVQLEQAQAAEDGAKATLINAE"
BASE COUNT          441 a          721 c          757 g          416 t
ORIGIN      
        1 gcccggcggc gcgaagtaag gcggcggaat tcgcggcttg aaaaagccgg gagcgccctc
       61 gagcagggtc gctcctgcgc gcaatcggtt gagaaagacg tgaccggtat cgccgcgcag
      121 ccagtaatgg gcgacgccga acgggtcgaa cagaagctgg gtcgtgacct gctcccaaaa
      181 ggcgcgcagc ttttcgagcc gcgcctcggg tgcgttgccg gcgatcaagg ccgcattgat
      241 cgcgccgatc gagattcccg cgacccaatc ggggtgcagg ttcgcctcgg ctaacccctg
      301 atacacgccg gcctggaagg cgccgagcgc gccgccccct tgcaatacca aggcgatgca
      361 gtcgaacggc ggccgcttca attcgcggtg gctcttcagg agcggcttga tgaaaggccg
      421 gctttgatct ttgggcgacc gcgtcgacga cttgacatcg acgggtgagg actcgcgctt
      481 cgcggttaaa ggcgcgacgc gggccggagc atcagtggag gtcatggcaa cgacgatccg
      541 tttttcacgt tccagcgctc tcgagttcga caggcaaagc ggattgatgg tcggtcgatc
      601 aaatcacgaa tacgtgcatg atttgtgtca cttctggtct tgccgaccgc cccccaaagg
      661 gtcttccgtc tgcgccgcgt cgcgccccat ctgcaggcag acgagaactt ccacatagct
      721 cggatcgccg ccggtttcgg tggtggtgac gcaacgctga cgcagggccg ccggaaactg
      781 cgtccactgc ttttccagct ggtcacgcgc gctcttctcg tccgccaggc attggcgaag
      841 cgtcgcatcc aagctcaagc tgtcgcccat tttcgccgcc gcacggcagc tagattcgac
      901 gtcgagggaa gggaccttgt cggcgacggt ccacagcaat gtaccaccga gcgcgagaag
      961 cgacgctgat ggaaacattc gggtctccag tcttcgcgcg cgccggctgc gcaatcgaaa
     1021 attgtgcgcc gacggtgaaa cgtcggcggt gcgccgcccc agccggccgg cagcggggca
     1081 tagcccgggg ccgcaggccg gtcaaccggc ttcgttgcat caaattagag catcgcgcag
     1141 cgctgtcgcc tcgtcgtgca aacgacgcgc actcggcaac tcgtgaccaa acgcgtttcg
     1201 cagcttgcta tcgtaccggg ggcgaagatg gcggactgag cttgcagctg tttagcacgg
     1261 cgtaatagcc cttctcgcct ttttggaagc ttaccgcggc ggtgaacacg taggtccggc
     1321 gcttgggctc cttgagctcg tgcacccacc aattcgtgac ttgacctggc tgactggaat
     1381 actgcgcgca ggttccgtcg ggtccgtcga cgagcggaac gctttgcatc tcgtagcgat
     1441 agtccttcgc atttggccaa gcgacgccga tggcgaacgt cggccgctcg taatccaagg
     1501 gaaaagcctg cgacacgttt tgcgggtcgc cgcagttcgg gaaggacgtc acgtattccg
     1561 gcctgccgac gcagatcatg atcggcgcgc cattgctcca gtcgatgccg ctgggcaggg
     1621 cgccccaatg ggtccggcag caggtctcga cctcaaccgc cggccatccc ggcggagttt
     1681 gaaacgatat gctctgggtg ctgatgcagt aattggtggt gcaggcatat tgcgcgctcg
     1741 ccggcgccgc gaccgagacg gcgagcaaag ccggcggcaa aaaatatcgc aacgtgttca
     1801 tagccagttt tgcaacagcg attcgcgcac gcatcatttt gtcctcatgc cggcccttgg
     1861 cgcttcggcg cgctgatgta cactaccaca tcgaatgcgc aaaggatcga gcagcgctcg
     1921 ctgcgcacgc acaggtggcg gagacgagga gatgagatgc gtccccaaag atctgttgcg
     1981 agcagcgcag gcgcggctgc tgcttctcgc cgggatgggg atgctcaccg tgctggcggc
     2041 gtgcgggcaa gagaaccgct tcatcgcgcc gccgccgccg aaggtgacgg tccagctgcc
     2101 cctgcaacag acgttcacgc cctatctgga ggcgaccggc aacgccgcct cagtgaatac
     2161 cgtcaagctg ttggcgcgcg tggaaggcta cgtgcaagag atcaagtatc aggacggcgc
     2221 cgcggtcaag aagggcacgc ctttgttcgt gatcgagccg gagccctaca aggtccagct
     2281 cgagcaggca caagcggcgg aggacggggc caaggcgacg ttgatcaacg ccgaa
//
LOCUS       DATLJT010001383         4718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2874218,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001383 DATLJT010000000
VERSION     DATLJT010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2874218"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..337
                     /locus_tag="VM910_31715"
     CDS             125..337
                     /locus_tag="VM910_31715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67107.1"
                     /translation="MQDDIPADTIDIIHARFPCHSPDLHFFGYRFRVILKGEFGDGDI
                     CWNMSTRQWSWRILPGQSLSRLNPRN"
     gene            1022..1627
                     /locus_tag="VM910_31720"
     CDS             1022..1627
                     /locus_tag="VM910_31720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67108.1"
                     /translation="MLDWAFNSASIEALAPFEELRGRHILALAIVGKFLRKTGARKDI
                     ADQFYALSAALNDLQRGIVHPMLAPKKPTGRLGDRSDIWRVRVNAGCGVECLMRSGVS
                     RDEAAQRAAKWFPALKNLLRTGTTLKTALLRWRDAVMRGPLNDVVARGAAQEFRDFLK
                     RVGHQLTREQFTALGEEYLKSASDRAATWLFASAPISADRS"
     gene            1743..3125
                     /locus_tag="VM910_31725"
     CDS             1743..3125
                     /locus_tag="VM910_31725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HVI67109.1"
                     /translation="MSMTPQSAMTSATQPTAIEHMPVASLRPYPGNARTHSKRQIRQI
                     ADSIRHFGFTNPVLVGENGEIIAGHGRVEAAKLLGLEKVPTVRLAHLNPAQRRAYVLA
                     DNKLALNAGWDRQLLGIELQGLIDIDFDVELTGFSSTEIDLVLDEAGEGSPNDPTDVE
                     DEIPLSTNDPATAITRTGDVWCLGRHRLICGDARDRVVFDLVMGHERADLIFTDPPYN
                     VPIDGHVTGLGRIRHREFAMGVGEMSADAFTSFLRQTLGHAAALACNGAIAFVCMDWR
                     HMGELLKAGQAVFSELKNLCVWNKTNGGIGTFYRNKHELVFVFEVGAAAHSNTFGLGD
                     RGHYRTNVWDYAGVKMLRVGRSEDLAMHPTVKPVALVAAAIKDCSKRGEIVLDPFGGC
                     GTTLIAAEKTGRLARLIEFDPAYCDTILHRYERVTGKQAKQAASGMSFEEIAKERAAS
                     LAAAEEAADR"
     gene            3149..3550
                     /locus_tag="VM910_31730"
     CDS             3149..3550
                     /locus_tag="VM910_31730"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HVI67110.1"
                     /translation="MLDSKRVGYGRPPVHSRFRKGQSGNPTGKRRHGEAERVEALIRQ
                     EVHRLVTVREGDTVTKMPALQAVIRSQIACAAKGNVTAQRALLKLVQNIEDARARTTG
                     QTANKKLHRDANEMSDKELMNILRAARNDKS"
     gene            3570..>4718
                     /locus_tag="VM910_31735"
     CDS             3570..>4718
                     /locus_tag="VM910_31735"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HVI67111.1"
                     /translation="MTAPEFGTLDVSPAVVAEILLKRRRVRGSLIEWCRHCGCEPAPH
                     HRLLIDRLERVARGEIKRLAVFMPPGSAKSTYGSILFPPYVMANAPKHAILAASHTTE
                     LAERWGRRVRNLIARHSATLGLRLSDDSHAAGYWELESGGEYYAAGVGAAIIGFRAHG
                     ALIDDPIRSREDADSPHVRDKIWEWYKSDLLTRLAPGGWVILIQTRWHEDDLAGRIIG
                     EMERGGERWDIVSLPAEAEAGDLLGREPGQWLWDDAYGYGDFLRHEKATQLPRNWSAL
                     YQQRPAPETGDYFKEEWFHTYARDPPRETLNIYGGSDYAVTSQGGDYTVHVVVGVDPE
                     NRMYLLDLWRGQTSSDVWIEAWCDLVCKWKPAFWAEEYGQIISGVGPYL"
BASE COUNT          968 a         1378 c         1494 g          878 t
ORIGIN      
        1 tcggcgctaa agcaaacatc gagcctcgac cacgcggggg agccgaccga tgcgccgcgc
       61 agcagcagtc cttgtggcat ttctgatatt gagcggatgc acccgtgagg aagcgagggc
      121 gcgtttgcag gacgatatcc cggccgatac catcgacatc atccatgcgc gatttccctg
      181 ccactcgccc gacctccact tcttcgggta ccgattccgc gtcattttga agggggaatt
      241 cggcgacggc gacatttgct ggaacatgtc gacgcggcaa tggagctggc gcatcctgcc
      301 ggggcagagt ctttcgcgcc tcaaccctcg caactagcgt ccgcgggtct tgagcgtttt
      361 tgatctttcc ggcgaccggg atggcatttg gaatccggct aggttgcgca aaatttagca
      421 aggatgagcg atttggccct ggctgaaaga tgttttgcat gtgacgtcac cgtcatccgc
      481 aattccctgc tagcagggaa tttagcaggg aattttttgg aaaagagccc ctgaggaacg
      541 attctcacgt ccaaagcgtg ggcagcatca aatggttacg ggcaaatcac ctgctacgga
      601 gcacggaatt tttattgacc gagcagggaa gttcatcgag ggagcaggga atgggaatgt
      661 cgcgccgggc gccgggcggc cgggcggatc ctaccctttg gttcgaacgg gacacggcgc
      721 tgataggcgg acagcgcatc atcgagggcg gcgcggttcg agagagaggg ccgagcatga
      781 ctgcggccca catcgatagg ccatcattgt caaagaaatg ccggtagact gaatcagcgc
      841 gcagttggcg ggccatcgcg atggtccttc acacatcacg tcgtgggcta tgaaaagctt
      901 ggtccgccga tcgcacgcga acccaaagcc ttccaagagg cgccgtggtg gcgccgcaaa
      961 gagccgcagg cacgctcgcc tggcgtcgag tagcgctgct gcccgcgctg aactttttcg
     1021 attgctcgat tgggcattta actctgctag catagaggct cttgcaccgt tcgaggaatt
     1081 gcgcggccgt cacattcttg cacttgcgat tgtcggtaaa ttcctcagaa agaccggcgc
     1141 tcgcaaggac atcgcggatc agttttacgc tttgagtgcc gcgctcaacg atcttcagcg
     1201 cgggatcgtt caccccatgc tggcaccaaa gaaaccaacc ggccgactcg gcgataggtc
     1261 tgacatttgg cgggtgaggg tcaacgcagg ttgcggagtg gagtgtttga tgcgcagtgg
     1321 cgtatcacgt gacgaagcgg cccagcgagc tgcgaagtgg ttccccgcac tcaagaacct
     1381 gctgcgaact ggtacgacgc tcaaaacagc actgcttcga tggcgggatg cggtcatgcg
     1441 cggtccgctg aatgacgtgg tggcgcgcgg cgctgcccaa gagtttcgcg atttcctgaa
     1501 gcgtgtcggc catcagttga ctcgggagca gtttacagct ctcggcgaag aatatctcaa
     1561 atcggcctcc gatcgtgctg ccacatggct ttttgccagt gctcccatat cggccgaccg
     1621 atcctgaaac gctcgcggcc aggtcgggcc gtcaaactat cccccgtatc ttagttcgac
     1681 ggcagtgctg gcatccttgg atgcctgaga cacaccgata cgaagcatta agaggcgcgt
     1741 gaatgtccat gaccccgcaa tccgccatga cgagtgccac ccaacctacg gccatcgaac
     1801 acatgccggt cgcgagcctt cggccgtatc ccggcaatgc ccgaacccac tccaagagac
     1861 agatccgaca gatcgccgat agcatcaggc atttcggctt cactaacccc gttctcgtcg
     1921 gagagaacgg cgagatcatt gccggccatg gccgggtcga ggccgcaaag ctgctcgggt
     1981 tggagaaagt cccgaccgtg cgcctggctc atctgaatcc ggcgcaacgg cgcgcctatg
     2041 tgctggccga caacaagctc gccctcaacg cgggctggga ccgacagctg ctggggatcg
     2101 agctgcaggg gctcatcgac atcgatttcg atgtcgagct caccggtttc tcgtccaccg
     2161 aaatcgacct cgtgctcgac gaagcgggcg agggctcgcc gaacgatccg actgacgtcg
     2221 aggatgagat ccccttatcc accaatgatc cggcgacggc tatcacgcgg acaggagatg
     2281 tttggtgtct cgggcgccat cgtttgattt gcggagatgc tcgagaccga gtcgtctttg
     2341 acctggtgat gggtcacgag cgggcggacc tcatattcac cgacccgccg tacaacgttc
     2401 cgatcgacgg gcacgtgaca gggcttggac gtattcgcca tcgcgaattc gccatggggg
     2461 ttggcgaaat gtcggccgat gcattcacaa gcttcctgcg acagacactc ggacatgccg
     2521 ccgcgcttgc gtgcaacggc gccattgcgt tcgtgtgcat ggattggcgc catatggggg
     2581 agctgctcaa ggcaggccaa gcggtctttt cggagctcaa gaacctctgc gtctggaata
     2641 agaccaacgg cgggataggg acattctacc gtaacaagca tgagctcgtg ttcgtgttcg
     2701 aggtcggcgc tgctgcccac agcaatacct ttgggctcgg ggatcgcggg cattaccgca
     2761 ccaacgtttg ggactatgcc ggggtcaaga tgctgcgagt cgggcgcagc gaagacttgg
     2821 cgatgcatcc gaccgtgaag ccggtggcat tggtcgccgc ggcgatcaag gactgctcca
     2881 agcgcggcga gatcgtgctt gatccgtttg gtggctgcgg cacgacgctg attgcggccg
     2941 agaaaaccgg ccgcctggcg cggctgatcg agttcgaccc cgcctactgc gacacgatcc
     3001 tacatcgtta tgagcgagtc accggcaagc aggcgaagca ggccgcgagc gggatgagct
     3061 ttgaggagat tgcgaaggag cgagcggcgt ccctggcggc ggctgaggag gcggccgacc
     3121 gatgagcaca aagaaaaagg aggcacccgt gctggacagc aagcgcgtcg gctatggcag
     3181 accgcctgtc cacagccgtt tccgcaaagg acaatccggc aatcctactg gaaaacgacg
     3241 gcatggcgaa gctgagcgtg tcgaggcgct catccggcag gaagtccatc gtttggtgac
     3301 cgtccgcgag ggcgacacgg tcaccaagat gccggcactg caggccgtca tccgcagcca
     3361 gatcgcatgc gctgcgaagg gcaatgtcac tgcccaacgc gccctcctca aactcgtgca
     3421 gaacatcgag gacgcccgag cacgcacgac cggacaaaca gccaacaaga aactccacag
     3481 ggacgcgaat gaaatgagtg acaaagagtt gatgaacatt ctcagagcgg cacgaaatga
     3541 caaatcctag tttcgcatac gaccaatcaa tgaccgcacc cgaattcggc acattggatg
     3601 tctcgccggc cgtcgtggcg gaaatattgc taaagcgccg ccgcgtccga ggaagcctca
     3661 tcgagtggtg ccggcattgc ggatgtgagc cggcgccgca ccacaggctg ttgatcgatc
     3721 gcctggagcg ggtggcgcgc ggcgagatca agcggctcgc agtgttcatg ccgccgggtt
     3781 cggcaaagtc gacctacgga tcgattttgt ttccacccta tgtgatggcg aatgccccga
     3841 agcacgcgat ccttgcggcg tcgcacacga ctgagctggc ggagagatgg ggccgcagag
     3901 ttcgcaacct gatcgcgaga cacagtgcca cgcttggcct gcggctttcg gacgacagcc
     3961 acgccgccgg ctactgggag cttgagagcg gcggggagta ctatgctgcc ggcgtcggcg
     4021 ccgccattat cggttttcgc gcccacgggg cattgatcga tgaccccatc cgctcgcgcg
     4081 aggacgccga ctccccacac gtgcgcgaca aaatctggga gtggtacaaa tcggacctcc
     4141 tgacgcgctt ggcgcccggc gggtgggtga tcctgatcca gacccgctgg cacgaggacg
     4201 accttgccgg gcgcatcatc ggcgaaatgg agcgcggcgg ggagcgctgg gacatcgtct
     4261 cgctgccagc cgaggccgaa gccggcgatc tcctcggtcg tgagccaggc caatggttgt
     4321 gggacgatgc gtatggctat ggagacttcc tgcgccacga gaaggcgacg cagctcccgc
     4381 gcaactggtc ggcgctctac cagcagcggc cggcgccgga aaccggcgat tatttcaagg
     4441 aagagtggtt tcacacctac gcgcgcgacc cgccgcgcga aacgcttaat atctatgggg
     4501 gatcagacta cgccgttaca tcgcagggag gtgactatac ggtgcatgtc gtggtcggcg
     4561 tcgacccaga aaaccgcatg tatctgctcg atctgtggcg gggccagaca tcctccgacg
     4621 tctggattga ggcttggtgt gacctggtgt gcaagtggaa gccggcgttc tgggccgagg
     4681 agtacgggca gatcatttcg ggtgtgggcc catacctt
//
LOCUS       DATLJT010001384         7147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1727040,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001384 DATLJT010000000
VERSION     DATLJT010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727040"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VM910_31740"
     CDS             complement(<1..353)
                     /locus_tag="VM910_31740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI67112.1"
                     /translation="MRHSEVPQAAQANSGDAELVRRALGRDEAAVRAILKANNQRLYR
                     LARGILRNDNEAEDVVQETYVRAFTHLADFRGASSLATWLSRIAMNEALGRLRRERPG
                     VELNALPQGTLEAQI"
     gene            complement(553..903)
                     /locus_tag="VM910_31745"
     CDS             complement(553..903)
                     /locus_tag="VM910_31745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI67113.1"
                     /translation="MSIQRFETGPRMSQVVVNGNTVYLAGVVANRAAGESVTKQTQDI
                     LNIIDGHLARAGTDKSKLLTATIYITDMRTFAEMNAVWDAWVSPGNTPARATVEARLA
                     APQYSVEIMVTAAK"
     gene            complement(1080..2027)
                     /locus_tag="VM910_31750"
     CDS             complement(1080..2027)
                     /locus_tag="VM910_31750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HVI67114.1"
                     /translation="MSRRTGNAQWLRASRSVVAGMLLFLACPAQAESISIDGVTRTFA
                     AQLSATKPAPLVIVLHGNMQSSADMASRTSWPVIARREQFSLLFPDGLNRAWEDLRPH
                     DKRAGRAPPDGTDDVAFIARLIEKYVADRTADPRRIYVTGLSGGGAMTMTLVCKRADL
                     FAAAASVIMNLTDEMAATCHPSRAVPILMMNGTADPLIPYEGGRGTSPFAIDGYWSTE
                     ETLLFWRRINGCEAGNAESVDLEDRDTKDQTTVTLVTSRCPHGRDLVMLYRINGGGHR
                     MPSSFPDTRFAGLINYMFGPQNHDIDGADVIWAFFKRFP"
     gene            2453..2920
                     /locus_tag="VM910_31755"
     CDS             2453..2920
                     /locus_tag="VM910_31755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67115.1"
                     /translation="MKLVTGLLAAVLLLAGVGASHAVVRIADDRGGRIGTYVDRYQGM
                     RTSGESVIIDGLCASACTIVLGAIPHDRICVTSHATLGFHAAWDFGANGRAITNPEAT
                     QMLYSMYPSAVRHWIARRGGLTPHMIFLRGKELQSMYRPCYLDAQASASKARW"
     gene            3005..3211
                     /locus_tag="VM910_31760"
     CDS             3005..3211
                     /locus_tag="VM910_31760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67116.1"
                     /translation="MAQPVSTQQTVSLVRPAGGGVRSAFIWGAVTLGLTLGVIVLLAA
                     LALWYHYGTRVFFETISAGISACF"
     gene            3225..3818
                     /locus_tag="VM910_31765"
     CDS             3225..3818
                     /locus_tag="VM910_31765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVI67117.1"
                     /translation="MDRTVARPLVIVTAFATSLLLGLLIMLWALGGVRGVPVPAAIGG
                     PFQLTDQSGTTVTDKSLQGRPSLIFFGFTHCPDVCPTSLFEISEVLRAMGKDADRVNA
                     YFVSVDPERDSKEAMKDYLSSFDPHLKGLTGDPEAIAKMLTAYRVYAKKVPLKDGDYT
                     MDHTALTYLMDRDGKFVAPFNLNRTPEEAAADLKRYL"
     gene            3912..4823
                     /locus_tag="VM910_31770"
     CDS             3912..4823
                     /locus_tag="VM910_31770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI67118.1"
                     /translation="MFHSLASVTSFSAAARRLAGRAAAACVVALALTGTGQAQTTPKP
                     AVEAAPQAVPGFWDPRRRPERPDLSRISVIRFLTETDYPPFNFTGADGNPAGFNVDLA
                     RALCEEIKVTCTIQMRRFETLLDALASNRGDAIIASMAVTPQLRTRVDFTDPYYRVPA
                     RFVSRRDAVMPEVRPEFLEGKKVGAIAGSAHEAYLKAMFTDGEVHSYPNDDALRLALR
                     RGEVDFIFGDAISLAFWINGTDSADCCAFSGGPFVESRFFGEGVGIAVRKGNDLLRQA
                     LNWALFRVWEKGRYTDLWLRYFSVSPF"
     gene            4965..6605
                     /locus_tag="VM910_31775"
     CDS             4965..6605
                     /locus_tag="VM910_31775"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVI67119.1"
                     /translation="MSVIDLAAKPGDLRALAEQSGAWPFEQAKAIVARLKKKPKDEVL
                     FETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKVKTRLIAFSDDMDGLRKVPDNV
                     PNKELLEKHLGQPLTTVPDPFGTHPSFGAHNNARLRAFLDTFGFDYEFASSTDYYKSG
                     KFDAALLRMLERLEKVMAIMLPSLREERAASYSPFLPICPRTGMVLYVPIVAHDAKAG
                     TVSYDDPDTNERVTLPVTGGHCKLQWKPDWAMRWYALGVDYEMAGKDLIDSVKLSGKI
                     CAALGGAPPDGFNYELFLDEKGQKISKSKGNGLTIEEWLRYAPPESLSLFMYREPKAA
                     KRLYFDVIPRNVDDYYQFLDAFPRQDARQQLGNPVWHIHAGHPPKADMPVTFQLLLTL
                     VSSSNAENAETLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPNKKFREPTES
                     ERAALQDLRDALSNLPATASAEDIQNVVYEIGRREPFLDPVKKGKDGRPGVSLDWFNM
                     LYQVLLGQEKGPRFGSFVAVYGIANAISMIDGTLARSA"
     gene            6750..>7147
                     /locus_tag="VM910_31780"
     CDS             6750..>7147
                     /locus_tag="VM910_31780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI67120.1"
                     /translation="MQFRVSPKTLSECSAEEWQARVDLAAAHRLAWMHGFSEGIFNHL
                     TLVVPGRSDRYYQIPFGMHWSEVTASSFMEVGIDDGEIKSGAGDVERSCYCIHAPIHK
                     ALPQAKAVFHTHMPFASALTRLEDPCIKEIG"
BASE COUNT         1251 a         2255 c         2238 g         1403 t
ORIGIN      
        1 atgatttgcg cctcaagcgt gccttgcggc agcgcgttca gctcgacgcc cggtcgctcg
       61 cgccgcaagc gcccaagcgc ctcgttcatc gcgatccgcg acagccaggt ggcaaggctg
      121 gacgcgccac ggaagtccgc cagatgggtg aatgccctga cataggtctc ctgaacgacg
      181 tcctcggcct cattgtcgtt gcgcaggatg ccgcgggcca gccgataaag cctttggttg
      241 ttcgctttga ggatggcgcg gaccgcggcc tcgtcacggc caagcgctcg ccggacgagt
      301 tcggcgtcgc ccgaattggc ctgggccgcc tgtggtactt ctgagtgtcg catggtggca
      361 tcgctgtcac ccaaaaaaat aggagcttaa gcgttggatg cggccgcggc aaaaaggttc
      421 ccgaagattg gtctcaatcg cttggcccgg caatggctcc gtcatttctg cccgacgcgc
      481 catgggcatg cgtgaaaaat gatgcggctg gcatcaagac agccgtagcc gcaccttccg
      541 aaacgccgcg gcttacttcg ccgcggtcac catgatctcg acgctatact gcggcgccgc
      601 cagtcgggcc tcgacggtgg cgcgcgccgg cgtgttgccc ggcgagaccc aggcatccca
      661 caccgcgttc atctcggcaa aagtcctcat gtcggtgatg tagatcgtcg cagtgagcag
      721 cttcgatttg tcggtgccgg ctctcgccag atggccgtcg atgatgttga ggatgtcctg
      781 ggtctgtttg gtgacgcttt cgccggccgc tctgttggcg acgacgcccg ccagatacac
      841 tgtgttgccg ttcacgacca cctggctcat gcgagggccg gtctcaaagc gctggatgct
      901 catgagatgc tccggttgaa tgcgacgcta acctggtgtc ccgattccga agttcggcaa
      961 cttgtcgatc cagtgcggcc ttggttcagg cgtccgcgtt gcgaatccgc ggcgaagtgt
     1021 ttgcggattt cgagccgcca cactagcgcg gcttgccgtc ccgagcaatc gcagcgcatt
     1081 cacggaaagc gcttgaagaa cgcccagatc acgtcggcgc cgtcgatgtc gtggttctgc
     1141 gggccgaaca tgtaattgat cagcccggca aagcgcgtgt cgggaaagct cgacggcatg
     1201 cggtggccgc ccccattgat gcggtagagc atgacgaggt cgcggccgtg cgggcaacgc
     1261 gaggttacca gcgtgaccgt ggtctgatct ttggtgtcgc gatcctcgag gtcaacggat
     1321 tcggcgttgc cggcctcgca gccgttgatg cgccgccaga acaaaagcgt ttcctcggtc
     1381 gaccagtagc cgtcgattgc gaaggggctg gtgccgcgcc cgccctcata gggaatgagc
     1441 ggatcggcgg tgccgttcat catcaggatc ggtaccgccc gcgagggatg gcaggttgcg
     1501 gccatctcgt ccgtgaggtt catgatgacg ctggctgctg ccgcgaacag gtcggcgcgt
     1561 ttgcagacaa gcgtcattgt catcgcaccg ccgccggaga gccctgtgac gtagatgcgc
     1621 ctgggatcgg ccgtgcggtc ggcaacatat ttttcgatca gcctggcgat gaaggcgaca
     1681 tcatcagtgc cgtcgggcgg cgcgcggccg gcgcgcttat catgcggtct cagatcttcc
     1741 caggcgcggt tgagcccgtc aggaaacagc agggaaaact gctcgcgtct tgcaatcaca
     1801 ggccatgacg tgcgcgaggc catgtcggcg ctgctctgca tgttgccgtg gaggacgatc
     1861 acgagcggcg cgggcttcgt tgccgaaagc tgtgcggcaa aggttcgtgt cacgccgtcg
     1921 atcgagatgc tctcggcctg cgccggacag gcgagaaaca ggagcatgcc cgcaacgacc
     1981 gatcgcgatg cgcgcagcca ttgcgcgttg ccggtccgcc tgctcatttt ctcgtcccgc
     2041 tgcctttgca ctgggcgccg gtcccgcctc gcctcgacta aaatccggcg tgccgagcgg
     2101 gtcaacggcg gatagcccag cccattgtca ggccatcgct ttccgttctc gttgtcgtgg
     2161 cgggcttagg cgatcgttaa cctgttccat tccggaacct ttttcgccgc cccaacagcg
     2221 ccccaaagcc atcgcaagtg cagcaggtct gcaacacctg acgtgaattt gttggggagc
     2281 atccgcgctg cctagacaaa ttcacgatct ggcaacaccc tctggcggtg tcccggtttt
     2341 gagcttttgc agtccgtttg cggcgggctc tcttgcgaat gctcgaaccg aaggacgcca
     2401 aaaggcatca cacggtcgga ctcgatttcg acggatcgaa ctggggtagg acatgaaact
     2461 ggtgacgggg ttgctcgcgg ctgttctctt gctggcgggc gtgggggcaa gtcatgcggt
     2521 ggttcgtatt gccgacgacc gcggtggaag gatcggaacc tacgtcgaca gatatcaggg
     2581 tatgcgcacc tccggcgaaa gcgtgatcat cgacggcctc tgtgcctcgg cctgcaccat
     2641 cgtcctcggc gccatcccgc atgacaggat ctgcgtgaca tcgcacgcga cgctcggctt
     2701 ccatgcggct tgggatttcg gcgcgaacgg acgagccatc accaatcccg aagccaccca
     2761 gatgttgtac tcgatgtatc catcggcggt gcgccactgg atcgcccggc gcggcggcct
     2821 gaccccgcac atgatcttcc tgcgcggcaa ggaattgcag tcgatgtacc ggccctgcta
     2881 cctcgacgcc caggcgtccg ccagcaaagc acgctggtag agcgcatttt catcagcatg
     2941 agttgatggg cagcacgcca gagcgtgctt gcccgcggcc gtctcgcacc ttaagtgagg
     3001 gggcatggcg caacctgttt ccacacagca gacggtctcc ctcgtccggc ccgcgggcgg
     3061 cggcgtgaga tcggctttta tttggggcgc cgtgaccctt gggctgaccc ttggagtgat
     3121 cgtcctgctg gccgcgctgg cgctctggta tcattacgga accagggtgt tcttcgagac
     3181 gatctccgcg ggcatttcgg cctgtttctg acgcagggac cagcatggat cggaccgtcg
     3241 cccgccctct cgtgatcgtc acggcctttg ccacgagcct cctgctcggg cttctcatca
     3301 tgctgtgggc gctcggcggc gtgcgtggag tgcccgtgcc ggccgcgatc ggcggcccat
     3361 tccagttgac cgaccagagc gggacgaccg ttaccgacaa gagcctgcag ggccgtccga
     3421 gcctgatctt cttcggcttc acccattgcc ctgatgtctg cccgacctcg ctgttcgaga
     3481 tttccgaggt gctgcgcgcc atgggcaagg acgccgaccg cgtcaacgcc tattttgtct
     3541 ccgtcgatcc cgagcgcgac agcaaggagg cgatgaagga ctatttgtcg agcttcgatc
     3601 cgcacctcaa aggcctgacc ggcgatcccg aggccatcgc caaaatgctc accgcctatc
     3661 gggtctatgc caagaaagtc ccgctaaagg acggcgacta cacgatggat cacaccgcgc
     3721 tgacctatct gatggaccgc gacggcaaat tcgttgcgcc cttcaacctc aatcggaccc
     3781 cggaggaagc cgccgccgac ctcaaacggt atctgtgatc cgcgctcgtt aaccgaacac
     3841 gggcctcgaa atcttgccgg gatggtctat aaggccgtcc aatccttatg cactcaccgg
     3901 ccagaccgtt gctgttccac tcgttagcca gtgtcacgtc gttttccgcg gccgcccgcc
     3961 gccttgccgg gcgggccgca gcggcatgcg tcgttgcgct tgcgctcacg gggacaggtc
     4021 aggcccaaac taccccaaaa ccagcggtcg aggcggcccc gcaggccgtg cccggcttct
     4081 gggatccgcg gcggcggccc gagcggccgg acctgtcgcg catctcggtg atccgcttcc
     4141 tgaccgaaac cgattacccg ccgttcaatt tcaccggcgc cgacggcaat ccggccggtt
     4201 tcaacgtcga tctggcgcgc gcgctctgcg aggagatcaa ggtcacctgc accatccaga
     4261 tgcgccgctt cgagacgctt ctcgatgcac tcgcaagcaa ccggggcgat gccatcatcg
     4321 cttccatggc ggtgacgccg cagcttcgca cccgtgtcga tttcaccgat ccctattatc
     4381 gcgtgccggc ccgcttcgtg tcgcggcgcg atgcggtgat gccggaagtg cggcccgaat
     4441 ttctcgaagg caagaaagtt ggcgccatcg cgggctccgc gcatgaggcg tatctgaaag
     4501 cgatgttcac ggacggcgag gtgcactcct atcccaatga cgacgcgctg cggcttgcgc
     4561 ttcggcgcgg cgaggtcgat ttcattttcg gcgacgccat ctcgctggcg ttctggatca
     4621 acggcacgga ttccgcggat tgctgcgcct tctccggcgg gccgttcgtc gaaagccgct
     4681 ttttcggcga gggcgtcggg atcgccgtgc gcaaaggcaa cgacctcttg cgccaggcgt
     4741 tgaactgggc gctgtttcgg gtttgggaaa aaggccgcta taccgacctg tggctgcgct
     4801 atttttcggt gagcccgttt tagcaaggcg tcgcctggcc cgctttcccg tcattgcgag
     4861 gcgcaaggcg acgaagcgat ccggcctttc ttcggagccc tgggttgctt cgctcgcaat
     4921 ggcggctgtg atagcttctg tttccttgga gaaccatacc tgacatgtcg gtcatcgatc
     4981 ttgcagccaa accgggcgac ctgcgcgcgc tcgccgaaca atccggcgcc tggccattcg
     5041 aacaggcgaa agcgattgtc gcgcggctga agaaaaagcc gaaggatgag gtgctgttcg
     5101 agaccggcta cggaccttcg ggcctgccgc atatcggaac attcggcgag gtcgcgcgca
     5161 ccacgatggt gcgccacgcc tttcgcgtgt tgaccgaaga caaagtcaag acgcgcctga
     5221 tcgccttctc cgacgacatg gacggcctgc gcaaggtgcc ggacaacgtg ccgaacaagg
     5281 aactcctgga aaagcatctc ggccagccct tgaccacggt gcccgatccg ttcggtacgc
     5341 atccaagctt cggcgcgcac aataatgcga ggttgcgcgc cttcctcgac acctttggct
     5401 tcgattacga attcgcgagt tcgaccgact attacaagtc cggaaagttc gatgccgcac
     5461 tcttgcgcat gctggagcgg ctcgaaaagg tgatggcgat catgctgccg agtttgcgcg
     5521 aggagcgcgc ggcaagctat tcgccgttcc tgccgatctg cccgcgcacc gggatggtac
     5581 tctatgtgcc gatcgtggcg catgacgcga aggccggcac ggtctcctat gatgatcccg
     5641 acaccaacga gcgggtcacg ctgccggtca ccggcggcca ctgcaagctg caatggaaac
     5701 cggactgggc gatgcgctgg tacgcgctcg gcgtcgacta cgagatggcc ggcaaggatc
     5761 tgatcgattc cgtgaaactg tcgggcaaga tctgcgcggc gctgggcggc gcgccgccgg
     5821 acggcttcaa ttacgagctg tttcttgacg agaagggcca gaagatctcg aagtcgaagg
     5881 gcaacgggct cacgatcgag gaatggctgc gctacgctcc gccggagtcg ctgtcgctgt
     5941 tcatgtatcg cgagcccaag gcggccaagc ggctttattt cgacgtgatc ccgcgcaacg
     6001 tcgacgatta ctatcagttc ctcgacgcct ttccgcgcca ggacgcgcgc cagcagcttg
     6061 gcaatccggt ttggcacatc catgccggcc atccgccgaa ggccgacatg cccgtcacct
     6121 tccagcttct tctgacgctg gtgtcgtcgt cgaatgcgga gaatgccgag acgctctggg
     6181 gcttcatcgg gcgctatcgc ccgggggtga cgccacaaac gcatccgaag ctcgatgcga
     6241 tggtgggtta tgccatcaac tactatcgcg acttcgtggc gccgaacaag aaattccgcg
     6301 agcccacgga aagcgagcgc gccgcgctgc aggatctgcg cgacgcgctg tcgaacctgc
     6361 cggcgaccgc atcggccgaa gacatccaga acgtggtcta cgagatcggc cggcgcgagc
     6421 cgtttctcga tccggtgaag aagggcaagg acggccgccc cggcgtgtcg ctcgactggt
     6481 tcaacatgct ctaccaggtg ctgctcggcc aggagaaggg tccgcgcttc ggctccttcg
     6541 ttgcggtcta cggcatcgcc aacgccatca gcatgatcga cggcacgctg gcgcggtctg
     6601 cctgaatttt ttctgtcgtc ccggctctgc gcttcgctcg ggcgggacga cggtgatatg
     6661 cgatggcgac aactctggcc cgctgacgaa ttgcgcaaaa ttgcgttata ctggtctttg
     6721 cataacgaca agtcagccgg gagcagcgca tgcaattcag ggtttcgcca aagacgcttt
     6781 ccgaatgcag tgccgaggaa tggcaggcgc gggtcgatct cgcggccgcg catcggctgg
     6841 cctggatgca cggtttctcc gagggcatct tcaatcacct gactttggtg gtgcccggcc
     6901 gcagcgatcg ctactaccag atcccgttcg gaatgcactg gtccgaggtg acggcatcct
     6961 ccttcatgga agtcggcatc gacgacggcg agatcaaaag cggcgccggc gacgtcgagc
     7021 gctcctgcta ttgcattcac gcgccgatcc acaaagcgct gccgcaggcc aaggccgtgt
     7081 tccataccca catgcccttt gcgagcgcgc tgacgcggct ggaggatccc tgcatcaagg
     7141 agatcgg
//
LOCUS       DATLJT010001385         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1496254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001385 DATLJT010000000
VERSION     DATLJT010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..545)
                     /locus_tag="VM910_31785"
     CDS             complement(210..545)
                     /locus_tag="VM910_31785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67121.1"
                     /translation="MGINRARQNKKTVAAVAFARRRRSRSDSLHQPVADENITTVNNS
                     IGKDDRTDEDLICHTLSFDALVPRNIGAAESLRTNALQLPAGTLDEPTPQIEPRWSEI
                     ADRKNQMEQ"
     gene            546..1727
                     /locus_tag="VM910_31790"
     CDS             546..1727
                     /locus_tag="VM910_31790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004108352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67122.1"
                     /translation="MHSRSQKGQEGFVWSTRSAAAGGVTTIVDMPYDDGCLIATAERF
                     GNKVSEANLQSRVDFALYATVDPQEGAARIGELVDAGTAAFKFSTFGTHPQRFPRIPP
                     QTLHACFSAIGPRGLFAGIHNESDEMVRAFTAEIQARGITDYRAHGLSRPPITESLAT
                     AEVYELGADTGCPVHIVHCSIGRGYELAAAYRAQGHAATIEACIHYLTLDEENDVRRL
                     AGKAKINPPLRSRREVEALWHHLAAGNVTVVSTDHVSWSEDRKSDPDMLKNASGVPGL
                     EVLYALLIKGLSERNLPLTHAARLLAHNPAALFRIAHVKGALAVGRDADVVLMRRDSY
                     RYRAADSGNNFVSWSPYDDIELPVRPVAAFLRGVCVAADGRVIAEPGHGRFVRPRLPA
                     H"
     gene            1793..>2835
                     /locus_tag="VM910_31795"
     CDS             1793..>2835
                     /locus_tag="VM910_31795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HVI67123.1"
                     /translation="MALAEITDPERPYTRRSFSPLFLQGRAWLAKRFADAGLAVRIDT
                     AGNLIGRLDGADPSLGVIAVGSHSDTVPSGGRFDGIAGLATGLEVVRALCDVGTRLGH
                     AIEVIDFLAEEPSEYGLSCVGSRGMVGALDAGMLDLTGPGGEKLREALRRVGGDPDRL
                     AASRRHDIKAFLELHIEQGIVLESQSLDVGVVTSIVGIRRIEIVFEGAADHAGTTPIT
                     LRHDALVAAANTVAAVRRIAEQLAAEGPDYFVATVGILSVEPGASNVVPARCRLVIDA
                     RSTNSALTRRFVEAVERDSAAYAAAAHVERTPLVTLSDAPPVACDPVLREALRRGAQE
                     LGLGETDLASGAG"
BASE COUNT          467 a          900 c          937 g          531 t
ORIGIN      
        1 tcaaaggata attggcctcg tccgccgcgg agaccgcgga agcggtcggc attagaagca
       61 gggctgctgt cagcaatctt gcggcaagcg tcactgcttc acgcatatat ccgccgatca
      121 aagggcatat ggcaggttgg cccacgggat tggtcgattc aacccctcgc caccaagcaa
      181 gccggcaagg caggctgcac atcattgcgt cactgctcca tttgattttt cctatcggca
      241 atttcgctcc atcggggttc gatttgtggg gtgggctcgt ctaacgttcc agcgggcaat
      301 tgcagcgcgt tggtccggag gctctcagcg gcgccgatgt tgcgaggcac caaagcgtcg
      361 aaggaaagcg tatggcagat caggtcttcg tcggtacggt cgtccttccc gatcgaattg
      421 ttgacggtgg ttatgttctc gtcggcgacg ggctggtgca ggctatcgga tcgggagcgg
      481 cgccgtcggg cgaacgccac ggcggcaacg gtttttttgt tctgcctggc gcgattgatg
      541 cccaagtgca cagccgtagc cagaagggcc aggagggctt tgtatggtcg actcgcagcg
      601 ccgccgccgg cggcgtgacg acgattgtcg acatgcccta tgacgacggc tgtctgatcg
      661 ccacggctga gcgcttcggc aacaaagtgt cagaggcgaa tctgcagtcg cgcgttgatt
      721 ttgcgctcta cgccacggtc gatcctcagg agggagcggc gcgcatcgga gagctggtcg
      781 atgcgggcac ggccgccttc aagttctcca ccttcggcac tcatccgcaa cgctttccac
      841 gcatccctcc gcagacgctc cacgcctgct tctccgcgat cggaccacgc gggcttttcg
      901 ccggcatcca caacgagagc gacgaaatgg tgcgcgcctt cacggccgag atccaggcac
      961 gcggcattac ggattaccgc gcacatgggc tgtcacgtcc gccaattacc gaatcgctgg
     1021 ccactgccga ggtgtacgag ttgggcgccg atactgggtg tcccgtgcat atcgtgcact
     1081 gctcgatcgg gcgaggatat gaactcgcag ccgcctatcg cgcgcagggg cacgccgcga
     1141 ccattgaagc ctgtattcat tatctcacgc tcgacgaaga gaatgacgtg cgtcgcctgg
     1201 ccggcaaggc caagatcaat ccgccgctga ggtcgcggcg tgaggtcgaa gcattgtggc
     1261 atcacctagc cgctggcaac gtcaccgtcg tctcgaccga tcacgtgagc tggtcagagg
     1321 accgtaagtc ggatccggac atgctcaaga acgcctccgg cgtaccgggc ctcgaggtgc
     1381 tctatgccct cctgatcaaa gggctcagcg agcgcaatct gccgctgaca cacgccgcgc
     1441 ggctgctggc tcacaatccc gccgcgctat ttcgtatcgc ccacgtgaaa ggtgcccttg
     1501 ccgtcggccg cgatgctgac gtcgtgctga tgcggcgcga ttcctatcgg tatcgcgccg
     1561 ccgacagcgg caacaatttc gtatcctgga gcccctatga cgatatcgaa ctgccggttc
     1621 ggccggttgc cgccttcctg cggggggtgt gtgtcgccgc ggacggccgc gtgatcgccg
     1681 agccgggcca cgggcgcttc gttaggccgc gcctcccggc gcattaaccg cggatccgaa
     1741 tttggcttcg accaacctgc cgcttgatgc cggtcgcctc tggaacgacg tgatggcgct
     1801 cgccgagatc acggacccgg agcgccccta tacccgccgc tccttcagcc ccctctttct
     1861 ccagggccgg gcctggcttg ccaagcggtt tgccgacgcg ggcctcgccg tgcgcatcga
     1921 caccgccggc aacctgatcg gacggctcga cggcgcggac ccctcgctcg gcgtgatcgc
     1981 ggtcggatcg cacagcgaca ccgtcccatc gggcgggcga ttcgacggga ttgcgggcct
     2041 cgcgacgggg ctcgaggtcg tgcgggcgct gtgcgatgtc ggcacacggc tcggccatgc
     2101 gatcgaggtc atcgactttc tcgcggaaga accaagcgaa tacggcctgt cctgcgtcgg
     2161 cagccgcggc atggtcggcg cgctcgacgc cgggatgctc gacctgacgg gaccgggagg
     2221 agagaagctt cgcgaggcgc tgcgccgtgt cggcggcgac cccgatcgcc tcgcggcgtc
     2281 gcggcgtcac gacattaagg cgtttctgga gttgcacatc gagcagggga tcgtgctgga
     2341 gtcgcaatcg cttgacgtgg gtgttgtcac ttcgatcgtc ggcatccgcc gtatcgaaat
     2401 cgtgttcgag ggtgcggccg atcacgccgg caccacgccg ataacgttgc ggcacgacgc
     2461 gctggtcgcc gcggccaaca cggtggccgc ggtgcgtcgt attgccgagc aattggccgc
     2521 tgaggggccg gactatttcg ttgccaccgt cggcattctc tcggtggaac cgggagcatc
     2581 gaatgtcgtg ccggcgcgct gccggctggt gatcgatgcg cggagcacca attcggcact
     2641 gacccggcgc ttcgtcgagg cggtggagcg cgacagtgca gcatacgcag cggccgcgca
     2701 cgtggaacgc acgccgctag tcacgttgtc cgatgcaccg ccggttgctt gcgatccggt
     2761 gttgcgcgag gcgttgcggc gcggggcaca ggagcttgga cttggcgaga ccgatctggc
     2821 cagcggtgca ggaca
//
LOCUS       DATLJT010001386         8416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1496360,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001386 DATLJT010000000
VERSION     DATLJT010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496360"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="VM910_31800"
     CDS             complement(<1..1513)
                     /locus_tag="VM910_31800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI67124.1"
                     /translation="MLRAASAVALAVLSIWIFGEPAFAGKRVALVIGNSAYRKVPLLA
                     NPARDADAMSEMFRNADFDVVQSKHDLGIGDMRRALRDFSDEASDADVAIVYYAGHGM
                     EVDGINYLIPVDAILERDIDAFDEAMPLDRVLTVIEPAKQLRLVILDACRDNPFRQTM
                     KRTVAARAVGRGLAMVEPINPNTLIAFAAKAGSTATDGDDKNSPFTAALLKHLPKPGL
                     DLRRAFGFVRDDVLKVTHNRQEPFIYGSLGGDDVALVPAPAVVDFNKAAKDDYELAQQ
                     INVISAWDSFIGKYPSGFYSDLARAQRDKLIAAKAAAEEADRLAAQKKALEETKAAEG
                     ERAKLAAQAKAAEDARILAEQAAAAEKAKAAEKAAEKAAAQARIEEAARVAGVLIENA
                     ATGKETKSDGPRVSAVCAGASTRLADISSRPAQTLSQAEECGLTPKEVFRECPNCPDM
                     VVVPAGEFLMGSSRNEIDNGLAAANEAPQHKVIINQPIAIGRFEVTRDQFAAFV"
     gene            1834..2016
                     /locus_tag="VM910_31805"
     CDS             1834..2016
                     /locus_tag="VM910_31805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67125.1"
                     /translation="MSAWSILVEGWLVLNAALFAALMFRRNQPALRDRLFRWVIYGSR
                     RTATFRHMPSHDSAET"
     gene            complement(2035..2334)
                     /locus_tag="VM910_31810"
     CDS             complement(2035..2334)
                     /locus_tag="VM910_31810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67126.1"
                     /translation="MIDQTPAAAPKRWWSKDSRISRTAEELELAITEAVKAAPGCEDF
                     VGVIIQGITPKSRLDVNWELRGTKFGHADRKIAREALTPIVERMQREFRLPDGPT"
     gene            complement(2345..2587)
                     /locus_tag="VM910_31815"
     CDS             complement(2345..2587)
                     /locus_tag="VM910_31815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67127.1"
                     /translation="MKFSRPVHYRACLPKAKEWSDVHLDFDPGRWRLACAAFADFAQI
                     FTESSNPWVFASDRIEASRESENDKNQLFVLGATST"
     gene            2982..3239
                     /locus_tag="VM910_31820"
     CDS             2982..3239
                     /locus_tag="VM910_31820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67128.1"
                     /translation="MDKEVVLVDWFTNESIGVRRLRPRQEPRKFDSRTAAVRFVMEEL
                     EKSGRHNVRMIVEGVEFSFVDIVRMYAARKKRLGYTSPSSA"
     gene            complement(3220..4575)
                     /locus_tag="VM910_31825"
     CDS             complement(3220..4575)
                     /locus_tag="VM910_31825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVI67129.1"
                     /translation="MSHRSGQYSEYAQHHPHDHGRPFVRRTHSHPQAATIAPSQSGYT
                     IVHAGKQVRFGPVVFWIVVGTVTLLGMWSAATATYFAFRDDVLTRLIARQAEMQYAYE
                     DRIADLRAKVDRTTSRQLLDQEQFDQKLEQIMRRQTALESRATALGTIPDVTGSIRPP
                     ARGAIAPEASPAAPKPSPISDTVIFVAPPDREARLESRAPTRAAPPQNQFAKIQGVDN
                     ILLRLQTSLDQVENRQMAALNSYEDGMESRVRRMRGVIGDLGLDMAQLEAATPRMPMG
                     GPFVPVKLAPDAGPFERQLYRINITRAQMERLNRTLALVPYRKPVVGEVEFTSGFGIR
                     SDPFLGRPAMHTGLDFRAQTGDPVRATANGKVVSSGWSGGYGRMVEIDHGNGLSTRYG
                     HLSEINVKVGDTVKIGQVIGEVGSTGRSTGPHLHYETRIDGEAVDPQKFLRAGVRLSS
                     G"
     gene            complement(4688..5380)
                     /locus_tag="VM910_31830"
     CDS             complement(4688..5380)
                     /locus_tag="VM910_31830"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI67130.1"
                     /translation="MSKKFRKKSPKSSRGRKARATAPPKPKAKAAEGRTSAAGKTRKN
                     TTSGPSHKAASKMLRSARLEASGGKSSVAGKTAGLAEGARAPTFRLPRDGGGSVALSD
                     FSGKKLVLFFYPKADTPGCTREAIDFTRLSKAFAESGTAVLGISADSVKRQETFRDKH
                     GLAVPLISDERHEMLEAYGAWGEKSMYGRTFQGILRTTVLIGTDGRVARIWRHVRVDG
                     HADQVLAAAREL"
     gene            5582..>8416
                     /locus_tag="VM910_31835"
     CDS             5582..>8416
                     /locus_tag="VM910_31835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HVI67131.1"
                     /translation="MSAQQRPLPVDGRGHGGDRFDNERQHQEAMARNSLPKGPYPGLS
                     AGVQPADWDDTDWDGDHDDVACHRARRLLSGSSSSIHRFGDKCAALRRWFVGERWVRR
                     LALVFAALVAIFVGCFFSLWWRLGAGPINLDMATPWLAAAIEENIGHDNTVEVGGTQI
                     ERAGRIRIAVRIRDIIVRDRDRAVVASAPKAEVKLSGAALLMGRLRAESLNLVDAELS
                     VRITPDGYVTVYTGDTAKPLATGVASKRLASDPSVSGRSPVPVPIPPPPAASPGGTAA
                     PPAKTDSTGSGLLAGLDWLDSLSLTGLDGQNLNEIGLKNGVLIVDDQQRGNKWRFENI
                     SLGLRRPSGGGVALSLGEEGAHPWWLRITVGPPENGVRSVDIRANNVPTGNILLALRL
                     KDLTYNADLPLTGELKGELGRDGLPTYFRGKLTAGAGSIIDTDTPDYPMEIDSAEINV
                     EWDSGRRVLVAPFKIISGANRVTLLAHLEPPNGSINDWQLGLSGGTIVLAGIDNEPPL
                     IFNRIAIGFRFDTARKQVLLTQADISNGEIGIAGTGSVDYSGEPLLKLGFAGTPMSAS
                     AMKRIWPTLIVPEVREWVIERIERGSVQRIEIGINTLVKNLPRKGPPIPDDGLSVNIV
                     ANGVAVHPVDGLPSVHDADLKAHVTGRTATVTIGQAVADTPGGRKLSISDFVFEVPDM
                     HPKPPPSRVKLRIEGPVPAAAEILASDRLSDLSATPVDPNTSKGTFSAIINMGMPVKG
                     ELTKENTTYNVTADLNGFAADKLVMSQKLEANSLKVVANNQGYQVKGDVKINGQAAAL
                     DYRKPAEGDADIRLQATLDDASRARLGLDLGPQVSGAIPIKLVGKISSDHDSKMGIEA
                     DLTQLKLDNILPGWVKLPGKSSRATFNVVPKTQSTRFEDVVIEGGGVSIKGSLEVDQN
                     GDLLNANFPTYSPSESDKTSLKAERGPDGVI"
BASE COUNT         1572 a         2577 c         2621 g         1646 t
ORIGIN      
        1 tcacgaatgc cgcaaactgg tcccgggtca cttcaaagcg gccgatcgcg atcggctggt
       61 tgatgatcac cttgtgctga ggagcttcgt tcgcggccgc caggccgttg tcgatctcat
      121 tgcggctcga tcccattaga aactcgcccg caggaacgac caccatgtca ggacaattcg
      181 ggcattccct gaagacttcc ttgggcgtga gcccgcattc ctctgcctgc gacagcgtct
      241 gcgccggtcg ggacgaaata tccgcaaggc gtgtagacgc gcccgcgcaa actgccgaca
      301 cgcgcgggcc atcactcttc gtctccttgc cggtggcagc gttttcgatc aggactccgg
      361 ccactcgcgc ggcttcctcg atcctggcct gggcggccgc tttttcagcc gccttttcag
      421 ccgccttggc tttttccgcc gccgcggctt gctcggccag aatcctggcg tcctccgcag
      481 cctttgcctg tgccgcgagt ttcgcccgtt caccctctgc ggccttggtc tcctccagcg
      541 cttttttctg cgctgccagg cgatctgcct cttcggcggc agccttcgcg gcgatcagct
      601 tgtcgcgctg cgccctcgct aggtcgctat aaaaaccgga ggggtatttg ccgatgaagg
      661 aatcccaggc cgaaatgaca ttgatctgct gggcgagttc gtaatcgtct ttcgcggctt
      721 tgttgaagtc gacgaccgcg ggcgccggca cgagcgcgac gtcgtcgccc ccaagcgagc
      781 catagatgaa aggttcctgt cggttgtggg tcaccttcag gacatcgtcg cgcacaaagc
      841 cgaatgccct gcgcaggtcg agaccgggct tcggcagatg cttcaacagc gccgccgtga
      901 acgggctgtt cttgtcgtcg ccgtcggtcg cggtcgagcc cgccttggcg gcgaaggcta
      961 tcagcgtgtt cggattgatc ggctcgacca tggccagccc gcgcccgacc gcgcgagcgg
     1021 cgaccgtacg cttcatggtc tggcggaacg gattgtcgcg gcatgcgtca agaatgacaa
     1081 gccgaagctg cttggctggc tcgatgaccg tcagcacgcg atcaagcggc atcgcctcgt
     1141 cgaacgcatc gatgtcgcgc tcgaggatcg cgtcgaccgg aatgagatag ttgatgccgt
     1201 cgacttccat cccgtgcccg gcatagtaga cgatggcgac gtcggcgtcg cttgcctcgt
     1261 cggagaaatc gcgcaaggcg cggcgcatgt cgccgatgcc gagatcatgc tttgactgga
     1321 cgacgtcgaa atcggcgttc ctgaacatct ccgacatggc gtcggcgtcc cttgccgggt
     1381 tcgcgagcag cggaaccttc cgataggcgg aattgccgat cacgagcgcg acgcgttttc
     1441 cggcgaacgc aggttcgccg aaaatccaga tcgacaaaac ggccaaagca acggctgagg
     1501 cggcacgcaa catgggtttg acccgtaaag tcccaaatat atcatagacc ttccggcgcc
     1561 cgacagttgt cggatggggg cttcgtgatt gcccgtgccc tggtctcgat gaccggctgg
     1621 tccattgcat tggtctggat cgcttacctg ttggtcggcg cccgttgtcc tcaaaaagac
     1681 actttgggca ggttccgtcg atggactcgg cagagcgatt ttgtcgccgt ctgttcctaa
     1741 tgccgaggcg cgtaaacctt gcccatcgcc cgcccgtctt gtgatcgcgt ctccaatggc
     1801 cgtgcccatc ttggcataaa aaggagaaca gccatgtcag cttggagcat cctcgtggag
     1861 ggctggctgg tgctcaatgc agctttgttc gcagcgctga tgtttcggcg caatcagccc
     1921 gcgctgagag atcgactatt ccgatgggtg atctatggca gccgtcggac tgccactttc
     1981 cgccacatgc cgagccacga tagcgcggaa acgtaagacc gcgcagacgg cctctcaggt
     2041 cggtccgtcg ggaagacgaa attcccgctg catgcgttcg acgatcggag ttaaagcttc
     2101 cctggctatc tttcgatccg cgtggccaaa cttcgtcccc cgaagctccc agtttacatc
     2161 caggcgcgat ttcggtgtta tgccctggat aatcacaccg acgaagtctt cgcagccagg
     2221 cgcagccttg actgcttccg tgatcgccaa ttcgagctct tcggctgtcc tcgaaatccg
     2281 gctgtccttg ctccaccatc tcttcggcgc cgcagcgggc gtttggtcga tcaaaggttg
     2341 cgcgtcaagt cgaagtggct ccgaggacga agagctggtt cttgtcgttt tcagattcgc
     2401 ggctggcttc gatacggtcg ctcgcgaaaa cccaagggtt ggagctttcc gtaaatatct
     2461 gtgcaaaatc agcaaaagca gcgcaagcca aacgccagcg cccgggatca aaatccaggt
     2521 ggacatcgct ccactccttc gctttcggaa ggcatgctct gtaatgcacc ggccttgaaa
     2581 acttcatttt tgatcttgag cgggcaaatc cccgctgcga agggcaaaaa tgggttcgtg
     2641 caagggaata gcggtttatt ttcgaagtcg ttgcatcact gcaacatttg aacagtgact
     2701 cgcagcacgg actactccgt tttcgctact gccagcccgg ggaaagagac ctacgctacg
     2761 gaccgggtgg cgggggtgcg tgaggtgccc tatgtcgaaa cgcggggaag agacgttcac
     2821 catgatcagc gcgcttgcag tctttgctgc gctctgtatt gcagcgctgc tgtggttgca
     2881 gtgattctac gccagcgtcg ctcgccagcc gccatcctgt atttgcgcgt gccccaatcc
     2941 cgccaagccc gaaagtctgc gtcgaccgaa tgagcacgcc aatggacaaa gaagtcgtgc
     3001 tggtggactg gttcacgaac gagtcgatcg gcgtcaggcg attgagaccg aggcaggagc
     3061 cgcgcaaatt tgacagccgc acggcagccg taaggttcgt catggaagag cttgaaaaaa
     3121 gcggacgcca caatgtccgg atgatagtcg aaggggtgga gttcagtttt gtcgatatcg
     3181 tgcggatgta cgccgcacga aagaagcgtt tgggctacac tagcccgagc tcagcctgac
     3241 gccggcacgc aggaatttct ggggatcaac cgcttccccg tcgatccgtg tttcatagtg
     3301 caggtgcgga ccggtcgagc ggcccgtcga tccgacttcg ccaatcacct gaccgatctt
     3361 cacggtgtcg ccgaccttta cattgatctc ggagagatgg ccgtagcgcg tcgacaggcc
     3421 gttgccatgg tcgatctcga ccatgcggcc gtaaccgccg ctccagcctg aggagaccac
     3481 cttgccattt gcagtggcgc gcacggggtc cccggtctgg gcgcggaaat cgaggccagt
     3541 gtgcatggca gggcggccga ggaacggatc gctgcgaatg ccaaagccgg aagtgaactc
     3601 gacctcgccg accacaggct tgcgataggg aacgagcgcc agcgtccggt tcagccgttc
     3661 catctgtgcg cgggtgatgt tgatgcgata aagctggcgc tcgaacgggc cagcgtcggg
     3721 cgcgagcttg accgggacga acggaccgcc catcggcatc cgtggggtgg cggcttcaag
     3781 ttgcgccatg tcgaggccaa gatcgccgat cacgccgcgc atgcgccgca cccgcgattc
     3841 catgccatcc tcatatgaat tgagcgccgc catctggcgg ttctcgacct gatcaagcga
     3901 ggtctgcagg cgaagaagaa tgttgtcgac gccctggatt ttggcgaatt ggttttgcgg
     3961 cggagcggcg cgcgtcggtg cccgcgattc caggcgggct tcgcgatccg gcggcgccac
     4021 gaagatcaca gtgtcgctga tcggcgaggg tttgggcgcc gccgggctcg cctccggcgc
     4081 gattgcgccg cgcgccggcg ggcggatcga gccggtcacg tccggaatcg tgccgagcgc
     4141 ggtcgcgcgc gactccaacg ccgtctgccg ccgcatgatc tgctcgagtt tctggtcgaa
     4201 ctgttcctgg tccagcaatt gccggctcgt ggtgcgatcg accttggcgc gcagatcggc
     4261 gatgcgatcc tcataggcat attgcatctc ggcttgccgc gcgatcagcc gcgtcaggac
     4321 gtcgtcgcga aaagcgaagt aggttgccgt cgcggcagac cacatgccga gcagggtgac
     4381 cgtgccgacc acgatccaga aaaccacggg accgaagcgg acctgcttgc cagcgtggac
     4441 gatagtatag cccgattgcg acggcgcgat cgtggccgcc tgcggatggg agtgcgtgcg
     4501 gcgtacaaag ggacggccat ggtcgtgggg atggtgctgg gcgtactcag aatactggcc
     4561 agaacgatgc gacatcggct ctcccgcgcc ggtcggatcc aggttccgta cggccaaatc
     4621 tgcgaaacgg atttgacagt cacatggtta attttccgac aacgcggact tgagcccaag
     4681 ggcgtcgcta gagctcgcgg gctgccgcga gcacctggtc ggcatggccg tcgaccctga
     4741 catggcgcca tatcctggcg acccggccgt cggttccgat cagcacggtg gtgcgaagga
     4801 tcccctggaa ggttctgcca tacatggact tttctcccca tgccccatac gcctcgagca
     4861 tctcgtgccg ctcatcggag atcaggggga ccgcgagccc gtgcttgtcg cgaaacgttt
     4921 cctgccgctt caccgagtcc gcggagatgc cgagcactgc ggttccgctt tccgcgaagg
     4981 ccttcgacag gcgcgtgaaa tcgatcgcct cgcgcgtgca cccgggagtg tcggccttgg
     5041 ggtagaagaa gagcaccagt tttttgccgg aaaagtccga aagggcgacg ctaccgccgc
     5101 cgtcgcgcgg cagacggaac gtaggtgccc ttgcaccttc agccagaccc gcagtctttc
     5161 ctgcaaccga actcttgccg cccgacgctt cgaggcgggc cgaccttaac attttcgatg
     5221 cggccttatg cgatggtccg gaggttgtat tcttgcgcgt tttgccggct gccgaagtgc
     5281 ggccctcggc ggcctttgct ttcggcttcg gcggcgccgt tgctctcgct ttgcggccgc
     5341 gcgacgattt gggggatttc ttgcggaatt tcttggacat acgccttcct ttcgtcgctt
     5401 tcggaagatc aatcattggg ctattgcggg tcccgtccgg cacgccggca aagatcggtg
     5461 gctgggcaag tctgacggcg ccggagtatg gttacacgga attcgatgcg gcgcctgtcc
     5521 tctcgttgaa tgagccaaaa gccggatgac gagcaacagg accaatcgag aattggcagc
     5581 aatgtcggca cagcagcgcc ccctacctgt cgacgggcgc ggccatggcg gcgatcgatt
     5641 tgacaatgag cgccagcacc aagaggcaat ggcaagaaat tcgctgccca aaggacctta
     5701 cccagggctg tccgccggtg tccaaccagc ggactgggac gataccgatt gggacgggga
     5761 tcacgacgac gtggcgtgcc atcgggcgcg tcggctgttg tcaggctcaa gctcaagcat
     5821 tcaccggttc ggcgacaagt gcgcagcctt acggcgctgg ttcgttgggg agcgctgggt
     5881 aaggcgactc gcgctcgttt tcgccgctct ggtcgcgatc ttcgtcggct gcttcttcag
     5941 cctgtggtgg cggctcggag cggggcccat caacctcgac atggcgacgc cgtggcttgc
     6001 cgccgcgatc gaggagaaca tcggtcacga caacactgtg gaggtcggcg gcacgcaaat
     6061 tgagcgggca gggcggattc ggattgcggt gcgcatccgc gacatcatcg tccgcgatcg
     6121 cgatcgcgcc gtcgtggcca gcgcgccgaa agccgaggtc aagctatcgg gcgccgcgct
     6181 gttgatggga cggctgcgtg ccgaaagcct caacctcgtc gacgccgagc tatcggtccg
     6241 catcacgccc gatggctatg tcaccgtcta taccggcgac acggcaaaac cgcttgcgac
     6301 cggcgtggca tcgaaacggt tggcgtccga tccgtccgtg tcgggtcggt cgccggtgcc
     6361 cgtgccgatc cctccaccgc cggcagcctc acctggtggc acggctgcgc cgcctgcgaa
     6421 aaccgactcg acgggcagtg gtctgttggc cggtctcgat tggctcgaca gtctcagcct
     6481 gacaggattg gacgggcaga acctcaatga gatcggtctg aaaaacggcg tcttgatcgt
     6541 cgacgaccag cagcgcggca acaaatggcg ctttgagaat atcagcctcg ggctgcgacg
     6601 gccgagtgga ggcggcgtcg ccttgagcct cggcgaggag ggcgcacatc cctggtggct
     6661 tcggatcacg gtcgggccgc ccgagaacgg ggtacgcagc gtcgatatcc gagcaaacaa
     6721 cgttccgacc ggcaacatcc tgctggcgct gcgactgaaa gaccttactt acaacgccga
     6781 tttgccgctg accggcgaac tgaagggcga attgggccgt gacggcttgc cgacttattt
     6841 ccgcggcaaa ctgacggctg gcgccggctc catcatcgat accgatacgc ccgattatcc
     6901 gatggagatc gattcggcgg aaatcaacgt cgaatgggat tccgggcgac gcgtccttgt
     6961 cgcgcctttc aagatcatat ccggcgcaaa ccgcgtcacg ctgctcgcac atcttgagcc
     7021 gcccaatggc agcatcaacg attggcaact cggcctttcc ggcggcacca tcgtactcgc
     7081 cggcatcgac aacgaaccac cattgatctt caaccgcatc gcgatcggct tccgcttcga
     7141 tactgccagg aagcaagtgc tcctgaccca ggccgatatc agcaacggcg aaatcggtat
     7201 cgccgggacc ggcagcgtgg actattcggg agaaccgttg ctcaagctcg gctttgcggg
     7261 aacgccgatg tctgcatcgg cgatgaagcg aatctggccg accttgatcg tcccggaggt
     7321 tcgcgaatgg gtgatcgagc gcatcgaacg aggctcggtg cagcgcatag agatcggcat
     7381 caatacgctg gtgaaaaatc ttccgcgcaa ggggccgccc attcccgatg acggcctttc
     7441 cgtcaacatc gttgcgaatg gcgtcgcggt gcatccggtc gacggcctgc cgtcggtgca
     7501 tgatgccgat ctcaaagccc atgtcactgg ccgcaccgcg accgtgacga tcgggcaagc
     7561 ggttgcggat acgccgggcg gccgcaagct cagcatttcg gattttgtct ttgaggtccc
     7621 agacatgcat ccgaagccgc cgccctcgcg ggtgaagctt cggatcgagg gcccggtacc
     7681 ggccgcggcc gaaatcttgg cctcggatcg tctcagcgat ttgtcggcga ccccggtcga
     7741 tcccaacacc agcaagggaa cgttctcggc catcatcaat atgggcatgc cggtcaaggg
     7801 cgaattgacg aaggaaaaca cgacctacaa cgtcaccgcc gacctcaacg gctttgcggc
     7861 cgacaagctg gtgatgagcc agaagctcga agccaactcg ctgaaggtcg tcgccaacaa
     7921 tcagggctat caggtcaagg gtgacgtcaa gatcaacggg caagcggcag cgctcgacta
     7981 ccgcaagccc gccgagggcg atgccgacat ccgcctgcag gcgacgctgg acgacgcgag
     8041 ccgcgcgcgg ctcggacttg atctggggcc acaagtgtcc ggtgccatcc cgatcaaatt
     8101 ggtcggcaag atctcaagcg atcacgacag caagatgggt atcgaggctg atctcactca
     8161 gctcaagctc gataatattc ttccaggttg ggtcaagctc cccggcaaat cgagccgcgc
     8221 gactttcaat gtggtgccga agacgcagtc gacccgcttc gaggacgtcg tcatcgaggg
     8281 tggcggcgtt tcgatcaagg gatcgctgga ggtcgaccag aatggcgatc ttctgaatgc
     8341 caacttcccg acctattcgc cgtccgaaag tgacaagacc tcgctgaagg ccgagcgcgg
     8401 cccggacgga gtcatc
//
LOCUS       DATLJT010001387         3281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4252627,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001387 DATLJT010000000
VERSION     DATLJT010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252627"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VM910_31840"
     CDS             <1..1050
                     /locus_tag="VM910_31840"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI67132.1"
                     /translation="DDVHPLIGHPTAAVLPAVLSIAEAKGRSGMDVLRAFIAGYEAAG
                     FVGSLVMRSHYERGFHSTATLGSFGAAAAAGLLLDLDESQMAVALGLAGTQAAGLKSM
                     FGTMAKPFHAGRAAANGVLAARLAARGFTANPGVLEVAQGFVATQSDGDPSVDVPLPR
                     PGSLVVQTLFKYHAACYLTHSSIEAIKLLRQRLSFSGPDVASIDIHVAPGHLSVCNIA
                     SPRSGLETKFSLRHTAALAACGRDTAAIQTYSDENALDPTLAMVRERVTVHGDRGAGR
                     DADVMIRTSGGQTAEATFDVAIPDRDLDRQGGRLVAKFHSLADPVIGAERASMLQRQI
                     AKLDEDHDIHVLMAA"
     gene            1265..2257
                     /locus_tag="VM910_31845"
     CDS             1265..2257
                     /locus_tag="VM910_31845"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67133.1"
                     /translation="MRRREFLGVLGSAGIAWPFAANAQQPDQMRTIGLLVAGTADDPE
                     YQARLGAFLQTLEQLGWTDGRNARIDTRWATTNADTLRKQAAELVALAPDVILAATGT
                     TTVAPLLEATRTVPIVFVLLIDPVGAGFVASLARPGGNATGFLMFEYGLSGKWLELLK
                     QIAPGVTRVAVLRDPAIASGIGQFGAIQSAAPTLGLEASPVNVRDAGDIERDIAAFAR
                     SSNGGMIVTASPRALLHRDLIVALAARHRLPAVYASRAYVTAGGLISYGPDILDQFRR
                     AAGYVDRILKGEKSADLPVQAPTKYELIINLRTAKALDFSFPPSLLARADDVIE"
     gene            2346..>3281
                     /locus_tag="VM910_31850"
     CDS             2346..>3281
                     /locus_tag="VM910_31850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67134.1"
                     /translation="MTPQIHGFLGPKSRSGVIVAGVMGAVLLLAAAWAAILWTRPEPV
                     RIAFASSLSGPSAPFGTESLVAAQLAIDEVNARGGINGRPIELVPFDDASSPETARAN
                     VQAIADSPCVAVLGHDLSSTSLAAGPAYKDARIPALTGGAAADDLTSGNEYYFRALSP
                     VSAQGRSIAEHLRALMKEPKVHLVHTRDSYGKNFLRGFAAAYPTEQLRAFGLDVAPGR
                     IGSMDEALDAAVQEPGPGIIVVGAATDFSADIVKALRRRGIKGTIVTPQGAAREGYLQ
                     NFANEPEEKAHPGFFSENLYAASSLIFDSAGGAAQV"
BASE COUNT          573 a         1036 c         1054 g          618 t
ORIGIN      
        1 gacgacgttc atccgctgat tggccatccc acggccgcgg tgctgccggc cgtactgtcg
       61 atcgccgaag caaaaggccg ttcgggaatg gacgtgctgc gcgctttcat cgcaggctat
      121 gaggcggccg ggtttgtcgg ctccttggtg atgcgctcgc actatgagcg cggctttcac
      181 tctaccgcta ctcttggctc gttcggtgcg gccgccgcgg ccgggctcct gctcgatctc
      241 gatgagtcgc agatggcggt tgcgcttggt cttgccggca cccaggcggc cgggctgaaa
      301 tcgatgtttg gcaccatggc gaagccgttc catgccggtc gcgcggcggc caacggcgtg
      361 ctggctgcac gccttgcggc gcgcggattc acagccaatc ctggcgtgct cgaggtggcg
      421 cagggtttcg tcgcgaccca gagcgatggc gacccttcgg tcgatgtgcc attgccgcgt
      481 ccgggcagcc tggtcgtgca gacgctcttc aaatatcacg cagcctgcta tctcacccat
      541 tccagcatcg aggcgatcaa gctgttgcgg cagcggctgt cttttagcgg ccccgatgtg
      601 gcgagcatcg acatccatgt cgcgccggga catctatcgg tttgcaacat tgcctcaccg
      661 cggagcgggc tcgaaaccaa gttcagcctg cgtcacaccg cggcacttgc agcctgcggc
      721 agagatactg ccgcgatcca gacttattcg gatgagaacg cccttgatcc gacgctcgcc
      781 atggttcgcg aacgggtgac cgtgcacggc gatcgcggcg caggccggga tgccgacgtc
      841 atgatcagga ccagtggcgg ccaaaccgct gaggctacct tcgacgtggc gatcccggat
      901 cgagatctgg accgccaagg cgggcgtctg gtggccaagt ttcatagcct cgccgatcca
      961 gtcattggag ccgaacgagc cagcatgctc cagcgccaaa tcgccaagct ggacgaggat
     1021 cacgacatcc acgtgctgat ggccgcatag atttcggagg gtgtccgctt cgatcagaat
     1081 ccaacagcgg cagcggtctg ccgttgagcg caaaccggaa aacatttgct cgcttttgca
     1141 ccttaccgct tttgaccccg gaatggacgt cggacagaat ccggtgagta gcgaagccgg
     1201 gttcggccac tacaaagagc tcatccgaag cgataagatg ctgtctcgtg ggccggaggc
     1261 ggacatgagg cggcgcgaat tcctcggtgt gcttggcagt gccggcatcg cgtggccgtt
     1321 cgctgcaaat gctcagcagc ctgaccagat gcgcaccatc ggcttgcttg tggcgggcac
     1381 tgcggatgat ccggaatatc aggcccgctt gggggcattc ctgcagacgc tggagcaact
     1441 gggttggacg gacggccgca acgcgcgaat cgatacccgg tgggccacga ccaatgccga
     1501 tacccttcgc aagcaagccg ccgaactggt cgcgctcgcg ccggatgtca tcctggctgc
     1561 taccggcacg acgaccgtgg cgccgttgct ggaagcgacc cgcacggtgc caatcgtgtt
     1621 cgtgcttctc atagatccgg tcggcgccgg tttcgtcgcg agcctggcgc ggccgggcgg
     1681 caacgccacc ggctttctca tgttcgaata cggtctgagc gggaaatggc tggaactgct
     1741 caagcagatc gcaccgggcg ttacacgagt ggccgttctt cgcgatcccg ccatagcctc
     1801 cggaatcggg cagttcggcg cgatccagtc cgcggcgccg acgctcgggt tggaggcaag
     1861 cccggtcaat gtgcgcgacg caggcgatat cgagcgcgac attgcggcct ttgcgcgttc
     1921 gtcgaatggc ggcatgatcg taacggcaag cccgagggct ttgcttcatc gcgatctgat
     1981 cgtcgcgctg gcggcccggc accgattgcc cgcggtctac gcctcccgcg cctacgtcac
     2041 cgccggcggc ctgatctcct acgggcctga tatccttgac cagttccggc gcgcggcagg
     2101 ttacgtcgat cgcatcctca agggcgaaaa gtcggcagac ctgccggtac aggcgccgac
     2161 caagtatgaa ctgatcatca acctaaggac tgccaaggct ctcgacttca gtttcccgcc
     2221 gtcgctactg gcacgtgcgg acgacgtcat cgaatgacac gcgtgctgtg ccgggctggc
     2281 tcaccggagg gaagccgaac ttgcacaaac accattcgat cagcactccc taagatgggc
     2341 gagcgatgac acctcaaatc catggttttt tgggaccgaa gtcacgctct ggagtaatcg
     2401 ttgccggcgt catgggcgcc gtcttgctgc ttgccgccgc gtgggctgcc atcctctgga
     2461 cccgcccgga gcctgttcgc atcgcgttcg ccagttcgct ctccggtccg tccgccccgt
     2521 ttgggacgga aagtctggtc gcggcgcagc ttgcaatcga cgaggtgaac gccaggggcg
     2581 gcatcaacgg acggccgatc gaactcgttc cgttcgacga cgcaagcagt ccagagacgg
     2641 cgcgcgcgaa tgtgcaagcg atcgccgaca gcccgtgcgt cgctgtgctc ggacatgatc
     2701 tcagctcgac ctcgctcgcg gcggggccgg catacaagga tgcacggatc ccggcgctca
     2761 cgggaggcgc ggctgccgac gatctgacat ccggcaatga gtactatttt cgtgcgctgt
     2821 cgccggtctc cgcgcagggg cgctcgattg cagaacatct gcgcgccctg atgaaggagc
     2881 cgaaagtcca tctcgttcat acgcgcgatt cctatggcaa gaattttttg cgcggatttg
     2941 cggcggccta tccgacggag cagttgcgcg cgttcggact ggacgtcgcc ccgggtcgga
     3001 ttggatcgat ggacgaggcg ctggacgccg ccgtacagga gcccgggccc ggcatcatcg
     3061 tcgtcggcgc ggcaaccgat tttagcgcag acatcgtcaa ggcgctgcgc cgccgcggca
     3121 tcaagggaac gatcgtcacg ccccagggcg cggcccgcga aggctatttg caaaatttcg
     3181 ccaacgagcc cgaggaaaaa gcgcatccgg gtttcttcag tgagaacctg tatgccgcat
     3241 cgtcgctgat ttttgacagc gccggcgggg cggcgcaggt t
//
LOCUS       DATLJT010001388         4005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1727749,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001388 DATLJT010000000
VERSION     DATLJT010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727749"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VM910_31855"
     CDS             complement(<1..592)
                     /locus_tag="VM910_31855"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67135.1"
                     /translation="MIDLYALTSPNVQKIYIMLEECGLPYKEHFVDVWKGEQYKPEFG
                     KINPNNKIPVIVDHDGPGGKPYTVIESGAILIYLAEKTGKFLPKDAAKKYDVLQWLMI
                     QLTGVGPMFGQWTHFKLFAPKGSGDYSLARYTSEIKRLYEVLETRLGKVPYLGGQDYS
                     IADMATFPWTRNHDAQGVKWEDNPNLARWFNAINERP"
     gene            1027..1752
                     /locus_tag="VM910_31860"
     CDS             1027..1752
                     /locus_tag="VM910_31860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HVI67136.1"
                     /translation="MTGRIAAENIKLMAVPRPPQGAVERLIALGDCTGVLSDTMDELG
                     IPCGVIGASVLRPTIAGKTIVGPALTVRNILQRINPIEGARQHVNRMAEFEAHNLAQK
                     GDVLVIQGVADVSNMGGISAQTGKRQGEIGAIVEGGVRDVAHSRALDYPVWASDITPV
                     TGKWRIETVEINGPVQIGEVRVAAGDLVVADDTGVCFIPRDVVLEVLDAAEQKAKAEA
                     VRCKAIDDGMPVPDIARATYGEK"
     gene            complement(1765..2229)
                     /locus_tag="VM910_31865"
     CDS             complement(1765..2229)
                     /locus_tag="VM910_31865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI67137.1"
                     /translation="MPRPPLPPFTAETAAQKARLAEDAWNSREPARVALGYTVDSVWR
                     NRAEFIHGREAIEAFLTRKWNRELDYRLIKEVWTYRDNRIAVRFAYEWHDDSGNWFRS
                     YGNENWEFDEDGLMRRRIASINDLPIKDGERKYHWPLGRRPDGHPSLSELGL"
     gene            2644..3615
                     /locus_tag="VM910_31870"
     CDS             2644..3615
                     /locus_tag="VM910_31870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67138.1"
                     /translation="MKLPRRKFLHLAAGAASISAVSRIAAAQAYPSRLVRIIVPFPAG
                     QATDTIARLMGQSLSERLGQPFVIENRTGAGGNIGTESVVRAAPDGYTLLLVGLSNAM
                     NATLYNKLNFNFIREIAPVASIGGAPYVMVINPSVPAKTVPEFIAYAKANPGKINMGS
                     SGSGSVSHVFGERFKMMTGINLVHVPYRGGYVPDLLSGQVQIVFGTISSCIQYIRGGM
                     LRALAVTTTTRSDVLPDIPTLAEFVPGYEANQWYGVGAPKDTPAEVIDKLNEEINAVA
                     ADPLIKARLASLGVDPMSMASAAFGKFIADEAEKWGKVIRAANIKSD"
     gene            3897..>4005
                     /locus_tag="VM910_31875"
     CDS             3897..>4005
                     /locus_tag="VM910_31875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HVI67139.1"
                     /translation="MTWSIIARDQATGQFGIAVASRFFAVGALVPRVKSR"
BASE COUNT          737 a         1235 c         1273 g          760 t
ORIGIN      
        1 cggggcgctc gttgatggcg ttgaaccagc gcgcgagatt gggattgtcc tcccacttca
       61 cgccctgggc gtcatggttg cgcgtccatg ggaaggtcgc catgtcggcg atggagtaat
      121 cctggccgcc gagatagggc accttgccga gccgcgtctc cagcacctcg tagaggcgct
      181 ttatttccga cgtgtagcgg gcgagcgaat agtcgccgct acccttgggc gcgaacagct
      241 tgaagtgggt ccattggccg aacatcggcc cgacgccggt gagctggatc atcagccact
      301 gcagcacgtc gtatttcttg gccgcgtcct tgggcagaaa ctttccggtc ttctcggcga
      361 ggtagatcag gatcgcgcca gactcgatca cggtataagg cttgccgccc ggtccgtcgt
      421 gatcgacgat cacggggatc ttgttgttgg ggttaatctt gccaaattcc ggcttgtact
      481 gctcgccctt ccagacgtcg acgaagtgtt ccttgtaggg cagcccgcat tcctcgagca
      541 tgatgtagat tttctgcacg ttggggctgg tgagcgcgta aagatcgatc atgaggattg
      601 gcctttgcta agagcgtttt ccgcgaaacg gactcgcggt tcgccgcgga caacgcatca
      661 acacgatgaa ctcgcgcgtt tccgattcca gtcatggaaa cgcgcgtccg agaggcggca
      721 acggtaccga gcggcgcgcg ccggagcaag cgccgaactg tacgatcaag cttacgagtc
      781 atgctcattt gtagggtggg cattgcgctc tcgcgctcgg ccatacaaag cggcagctcg
      841 gagcaatgcc cacgcgtcct gtcggcgcgg gcaaaatcgc cgcggcgtca cctgccatgc
      901 cttggccggc gattttgccc accccgcacc gcgctccttt ttcaccttcc gagatagata
      961 tagagtgcgc gacgcggcaa gctttagacc gggagcgcct ccgtgaacgc ggcagagaag
     1021 aagcggttga ccggccgcat cgcggccgaa aacatcaagc tgatggcggt cccgcgaccg
     1081 ccccagggcg cggtcgagcg cctcatcgcg ctcggcgatt gcaccggcgt cctctcggac
     1141 acgatggacg agctcggcat cccctgcggc gtcatcggcg cgtcggtgct caggccgacc
     1201 attgcgggca agaccatcgt cgggccggcg ctgacagtgc gcaacatcct ccaacgcatc
     1261 aacccgatcg aaggcgcgcg ccaacacgtc aaccgcatgg ccgagttcga ggcgcacaat
     1321 ctcgcgcaga aaggcgacgt gctggtgatc caaggcgtgg ccgacgtctc gaacatgggc
     1381 ggcatctccg cccagaccgg caagcgccaa ggcgagatcg gcgcgatcgt tgagggcggc
     1441 gtgcgcgacg tcgcccattc ccgcgcgctc gactatccgg tgtgggcgag cgacatcacg
     1501 ccggtcaccg gcaagtggcg gatcgagacc gtggagatca acggcccggt tcagatcggg
     1561 gaggttcggg tcgcggcggg cgacctggtg gtcgccgacg acaccggcgt ctgcttcatt
     1621 ccgcgcgacg tcgtgctcga agtgctcgac gcggccgagc agaaggcgaa ggccgaagcg
     1681 gtgcgctgca aggcgatcga cgacggcatg ccggtgcccg atattgcgcg cgcgacctac
     1741 ggggagaagt aggcggatag tcccttaaag gcctagctca ctgagcgatg ggtgaccgtc
     1801 gggacggcga ccgaggggcc agtgatattt gcgctcgcca tccttgatcg gcaggtcgtt
     1861 gatgctggcg atgcgccgcc gcatcaggcc gtcctcgtcg aactcccagt tctcgttgcc
     1921 atagctgcga aaccagttgc cggaatcatc gtgccattca tatgcaaagc gcacagcgat
     1981 ccggttgtcg cggtacgtcc acacttcctt aatcagtcga taatcgagct cgcgattcca
     2041 tttgcgcgtg agaaacgcct cgatcgcctc gcggccgtga atgaactcgg cgcggttgcg
     2101 ccagacgcta tcgacggtat agccaagggc gacgcgggcg ggctcgcggc tgttccaggc
     2161 atcttcggcg aggcgcgcct tctgcgcggc agtctcggct gtgaacggcg gcagcggtgg
     2221 gcggggcatc gactgactcc gacttgttca cgttcaagcg tccttgcgcc cagacctttc
     2281 tgcgtggaag tcggctcctg gccgcttcct gggtcaccca gcgaaaagat agcacatgac
     2341 aaccttggtg agagaccgct gcactcgcct tacctgccga cctctgcgag aggattaggt
     2401 gccacaaacg gcggattggt gctttctcca accactgtgg gcgcggtccc gcccggctct
     2461 agaaattgac gaaacgcttc caacccacat ggcggttggg gtgctcgccc ggcttagcat
     2521 cctgcgcctg caggcgcttg cacgaatggc ccgttgtgca tcacggcgaa attggcggcc
     2581 gatggcactg cggtaggtgc gacggcatga tactctgtcc ccgcgggaca gcctggggac
     2641 gccatgaaac ttccccgccg caaattcttg catctggccg cgggcgctgc ctcgatttcg
     2701 gccgtgtcgc gcatcgcggc ggcgcaagcc tatccgtcgc ggctagtgcg gatcatcgtc
     2761 ccctttcccg ccggccaggc aaccgacaca atcgcccggc tcatgggtca atccctgtcg
     2821 gagcggctcg ggcaaccatt cgtcattgag aaccgaacgg gtgcgggcgg caatatcggc
     2881 accgagagcg tcgtgcgggc agcgcctgac ggctatacgc tcctgctcgt cgggctgtcg
     2941 aacgcgatga acgcgacgct ctacaacaag ctcaatttca atttcatccg cgaaatagcg
     3001 ccggtcgcga gcattggcgg cgccccttat gtcatggtga tcaacccatc cgttccagcc
     3061 aagacggtcc cggagttcat cgcctacgcc aaggccaatc cgggcaagat caacatggga
     3121 tcgtcgggca gcgggtccgt gtcccacgtc ttcggcgaac ggttcaagat gatgaccggt
     3181 atcaacctgg tccacgttcc gtatcgcggc ggttacgttc ccgatctgct gagcggacag
     3241 gtgcagatcg ttttcggcac catatcctcg tgcatccaat acatcagggg cggcatgctg
     3301 cgcgcgctgg cggtgacaac cacgacccgt tcggatgtgc tgccggatat tccgaccctg
     3361 gccgaattcg tgcctggcta cgaggcgaac caatggtatg gggtcggcgc gccgaaggac
     3421 acgcccgccg aggtgatcga caagctcaac gaggagatca acgccgttgc ggcagatccc
     3481 ctcataaagg cgcggcttgc cagcttgggt gttgacccga tgtcgatggc atcggccgcg
     3541 ttcgggaaat tcatcgccga tgaagccgag aaatggggca aggtgatccg ggcggccaac
     3601 atcaagtccg attaggtgtg ccgcgctgat atttgggatg ttcgagaaac atcgatgtgc
     3661 ggcattgctg gcgagtggga ttggaaaggc aaaccgcgaa cgccgaacgt tctcgccgct
     3721 ataatcgcgg cagacctcgc gccgtcgcat ccaacgccgc accttgccgg cctgtgtgac
     3781 agaagaaacg ggatgcgata gactgacgtt gctacccaaa gggcgtgggt tacgtgcatc
     3841 gctgaatagc gcgcccagtt tgaacccttt gatcgccgta ccggtggatg gctggcatga
     3901 cctggtcgat catcgctcgc gatcaggcca ccggacaatt cggaattgcc gttgcgagtc
     3961 gtttcttcgc tgtgggcgcg ctcgttcctc gcgtcaaatc gcgca
//
LOCUS       DATLJT010001389         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1956159,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001389 DATLJT010000000
VERSION     DATLJT010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956159"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..403)
                     /locus_tag="VM910_31880"
     CDS             complement(8..403)
                     /locus_tag="VM910_31880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI67140.1"
                     /translation="MDLLTRLKAQPLPFAALLGIDLRSAAPEKIVGEMTVRPEFCTRP
                     AVLHGGAIMAFADTLGALATIANLKDGAGTTTIESKTNFIGAATVGSRVVGETTPVHR
                     GARTMVWQTRVTTTEGKLVAIVMQTQLLL"
     gene            complement(548..1177)
                     /locus_tag="VM910_31885"
     CDS             complement(548..1177)
                     /locus_tag="VM910_31885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVI67141.1"
                     /translation="MTSDEIEGEKLRPVHALQTAIARWLARAVPVAVAALMCASAALN
                     AAPLRIVAIGASNTHGFYVGNEGAYPAQLQALLRAKGIDAQVVNAGVPLETTGMMLRR
                     IDRDVPDGTNIVILEPGGNDRRFLVPPQWRAANIAEMERRLRARSINVIVYDEWIPLR
                     YRTLDFIHLTHEGHAMIAAALLPRVMEILDRRRNDVPPRRNASRPAPTR"
     gene            complement(1427..1717)
                     /locus_tag="VM910_31890"
     CDS             complement(1427..1717)
                     /locus_tag="VM910_31890"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI67142.1"
                     /translation="MKAYAIAAETIKDQAMFDAYRKVVPETLVPFGGQFIARGGNLTV
                     LEGEWPHPRLVIIEFPSRAAAEGWYRSAAYQKVISLRLNSSVGNLIIVDGSA"
     gene            complement(1763..2926)
                     /locus_tag="VM910_31895"
     CDS             complement(1763..2926)
                     /locus_tag="VM910_31895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67143.1"
                     /translation="MWRALTALAALALASANAVAEETVKVGLILPMTGPFTTTGKQVE
                     AGGRFYMQQNGATVDGRKIELILRDDAGVADNSRRIAQELLVSDKVNVLAGFGLTPIA
                     LAVAPLATETKTPMVVMAAATAIVTERSPYIVRSSFPQAAPVVIIADWEAKHGLKTVA
                     TVVSDFAPGHDSETYFKQTFTRAGGEVPLALRVPLLNPDFAPFLQRVRDAAPDGVFVF
                     IPAGQAAAMMRQFVERGLDKSGIRLFGAGDITDDDLLNNMGDVALGIETAYFYSAAHP
                     SEKNKAFVEGVKRANNGMRANFFGVSGYDGMHLIYEAIRKTGGKTDGDSFIGAVKGMS
                     WESPRGPMTIDPQTRDVIHNIYMRRVERVNRELWNVEFATVENVKDPVKATRK"
     gene            complement(3230..4063)
                     /locus_tag="VM910_31900"
     CDS             complement(3230..4063)
                     /locus_tag="VM910_31900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HVI67144.1"
                     /translation="MSEMAIEAALRRDRAIVAAALIILTALTWAYVWRLAADMDMGDM
                     DMSGFRIIPAGMGLMMPATAPWNTVEFAFVFAMWAVMMIGMMTPSATPMILIYARVGR
                     QAARQGTPLAASAYFAAGYLLSWIGFALVATSAQWALERAALLTPMMAGASDMFSGAV
                     LVAAGFYQWTPLKDACLRQCQAPWLFIQRHGGFRSDASGALALGARHGAYCIGCCWAL
                     MALLFVGGVMNVLWIAAIAILVLAEKVIPAGRVISRIAGAGLFAGGAWVLVQALPQLG
                     G"
     gene            complement(4070..4705)
                     /locus_tag="VM910_31905"
     CDS             complement(4070..4705)
                     /locus_tag="VM910_31905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HVI67145.1"
                     /translation="MADTKWTIKGREFVNCNCSYGCPCQFNGLPTHGHCQAVGGFEIE
                     QGHHGSTKLDGLKFVGIFRWPGAIHEGKGEAAVVIDERATEPQRAALLRILTGQDTEP
                     GKTIFNVFASTFEKLHDPIFAPIEFQVDVAGRKARLVVPGVTEGRGEPILNPVTGAQH
                     RVRIDMVDGFEYTLAEIGRGWNKTSRPIRLELADTYGQFAEIHLCQSGIVR"
BASE COUNT          912 a         1567 c         1518 g          923 t
ORIGIN      
        1 agcggcgtca tagaagaagc tgcgtttgca tcacgatcgc aacgagcttc ccctcggttg
       61 tcgtcacccg cgtttgccac accattgtgc gcgctccgcg gtggaccggc gttgtctcgc
      121 cgacaacgcg gctgccgacc gttgccgcgc cgatgaagtt ggttttgctt tcgatcgtgg
      181 tcgtgcccgc gccatccttc aaattcgcga ttgtcgcgag cgcaccgagc gtatcggcaa
      241 aggccatgat ggcgccccca tgcagaaccg ccgggcgcgt acaaaactcg ggccggacgg
      301 tcatttcgcc gacgatcttc tccggcgcgg cgctgcggag atcgatgccg agcagtgcgg
      361 caaaaggcag tggctgcgcc ttcagtcgtg tcagcaggtc cattcatcgc ctccttgcgc
      421 gtggccgaaa gacggctcct ctcaaatgct ccgtttcgac gcagcgagct tttgacgcct
      481 tgatgccgca gcgtgggcgc cgtccacggc atcaatcgcg ccggaccaac gcagccaggg
      541 gcttcgatta cctcgtcggc gcgggccggg atgcattccg gcgcggcgga acgtcattgc
      601 gtcgacgatc gaggatttcc atgacgcgcg gaagcaaggc ggcggcaatc atggcgtgac
      661 cttcgtgcgt gagatggata aagtcgaggg tgcgatagcg cagtgggatc cattcatcgt
      721 atacaatcac attaatcgag cgagcgcgca gccgccgctc catctccgca atgtttgccg
      781 cgcgccactg cggcgggacg aggaaacgcc gatcgttgcc gcccggctcc aggatcacga
      841 tgttcgttcc atccggcacg tccctgtcga tgcggcggag catcatcccc gtcgtttcga
      901 ggggcactcc ggcattgacc acctgagcat cgatgccctt ggctcggagc agggcctgca
      961 actgcgcggg atatgcgcct tcgttgccga cataaaagcc atgggtgttg ctggcgccga
     1021 tcgcaacaat gcgcagcggc gccgcgttga gggccgcgct tgcgcacatc agtgccgcga
     1081 ccgctacggg gaccgctctg gcgagccagc gcgcgatggc tgtttgcagc gcgtggacgg
     1141 ggcgcaattt ttccccttct atctcgtccg aagtcaaccg aacaatcctc tttcccttgg
     1201 cgaaacgaag acggccgtgc ctgcgcgcac gctgttccac aggaagctag cagccctcgg
     1261 ggcggatacc tcaaacgata gcacattagt ttgttgcaag tcagagaacc tctcactgcg
     1321 ccggggatgg cccttgcgtg agagaggacg cgcggctaag cgcgtcgccg ccttatctcg
     1381 cgaagcggac atcgcgagat gcgctccgtt gtgcgtgagg cttggctcag gcggacccgt
     1441 cgacaatgat caaatttccc acggaactgt tcagcctcag cgagattact ttttggtacg
     1501 cggctgacct gtaccaacct tctgcggcgg cgcgtgaggg gaattcgatg atcaccaacc
     1561 gaggatgcgg ccattctcct tcgagcacgg tcaggttgcc tccgcgggcg atgaattgcc
     1621 cgccgaatgg aacgagcgtc tccggcacca ctttgcggta tgcatcaaac atcgcttggt
     1681 ccttgatcgt ctctgcggcg atcgcatagg ccttcatgaa gaactccctc gctgacgtga
     1741 cccgtaccag ctccaccgca ggctatttcc gcgtcgcctt gaccggatct ttgacgttct
     1801 ccacggtcgc gaactcgacg ttccagagct cgcggttcac ccgttcgacc ctgcgcatat
     1861 aaatgttgtg aatgacatcg cgcgtttgcg ggtcgatggt cattggcccg cgcgggcttt
     1921 cccacgacat gcccttcacg gcgccgatga agctgtcgcc gtcggtcttg ccgcctgttt
     1981 ttcggatcgc ttcatagatg agatgcatgc cgtcgtagcc tgacacgccg aaaaaattcg
     2041 cccgcatgcc gttgttcgcc cgcttcaccc cctcgacgaa agccttgttc ttctcggagg
     2101 gatgggcggc ggaataaaag tacgcggtct cgatgccgag cgcgacgtcg cccatgttgt
     2161 tgaggaggtc gtcgtcggta atgtcgcccg ccccgaacag gcggatgccc gatttgtcga
     2221 gcccgcgctc gacgaattgc cgcatcatgg cggcggcctg gccggccgga atgaagacga
     2281 aaaccccgtc cggggcggca tcgcgcacgc gctgcaggaa cggcgcgaaa tccggattga
     2341 gcaaaggcac ccgcagcgcc agggggacct cgccgccggc gcgggtaaac gtttgcttga
     2401 aatacgtttc ggaatcgtgg ccgggcgcga agtcggaaac gaccgtggcc actgtcttga
     2461 ggccatgttt ggcctcccaa tcggcaatga tcacgaccgg cgcggcctga ggaaagctcg
     2521 agcgcacgat atagggcgag cgctcggtca cgatcgcggt ggccgccgcc atcaccacca
     2581 tgggcgtctt ggtctcggtt gcaagcggcg cgacggcgag cgcgatcggg gtgagcccga
     2641 aaccggcgag aacattcacc ttgtcgctga ccagaagctc ttgggcaatg cgcctgctgt
     2701 tgtcggcaac gccggcgtca tcgcgcagga tgagctcgat tttcctgcca tcaaccgttg
     2761 cgccattctg ttgcatatag aaccggccac ccgcttcgac ttgcttgccc gtcgtcgtaa
     2821 acggccccgt catcggaaga atgagaccga ccttgacggt ctcttcggcc actgcgttcg
     2881 ccgaggccaa cgcaagagct gccagagcgg tcagcgcccg ccacattgat gtgtcctcca
     2941 attttattcg tgatcagcgt aacgcatggc ggtttgctgc gccatcgcac atcgaggtga
     3001 cgacccagtc gcggagttca cggcgagttt gggcttgagc tgacggattt tgatgtcggc
     3061 gcccgccggc ggcgcctaag agctgtaggg tgggcaaaat cgtttgcggc accgtcgccg
     3121 cgtggggcca tcagggtgtt tacgcccatc ttcgccgggc tatgggcgcg tgcgcacgat
     3181 tttgcccacg cgagcagtcg agcaacgcgc ctttgcccac ccgacgcgac tatccaccca
     3241 attggggaag tgcttgtacc agcacccatg cgccgcctgc gaaaagcccg gcacctgcga
     3301 tgcgcgaaat cacacgcccg gcgggaatga ccttctccgc cagtaccagt atcgcgatcg
     3361 cggcgatcca cagcacgttc atcactccgc cgacgaacag taatgccatc aaggcccagc
     3421 agcagccgat gcaataggcg ccgtgacggg caccgagcgc gagcgcgccg gacgcgtcgg
     3481 agcgaaatcc gccgtggcgc tgaatgaaca gccagggcgc ctggcactgt cgtaggcagg
     3541 cgtccttgag cggcgtccac tgataaaagc cggcggcgac cagcaccgcg ccgctgaaca
     3601 tatcgctcgc gcccgccatc atcggcgtga gcaaagcggc ccgttccagc gcccattgcg
     3661 ccgacgttgc gacgagggca aagccgatcc agctgagaag atagccggcg gcaaaatagg
     3721 cgctcgccgc caatggcgtc ccctgtcgcg ctgcctgccg gccgacacgg gcatagatca
     3781 agatcatggg cgtcgccgat ggggtcatca tgccgatcat catgacggcc cacatggcaa
     3841 acacgaacgc gaactcgacg gtgttccagg gcgcagtggc cggcatcatc aggcccatgc
     3901 cggcagggat tatccgaaag ccggacatgt ccatgtcgcc catatccatg tccgcggcga
     3961 gccgccacac gtaagcccag gtgagcgcgg tcaggatgat gagggcggcg gctacgatcg
     4021 ctcgatcgcg ccgcagcgcg gcctcgatgg ccatctcaga catctcggct cagcgcacga
     4081 tgccgctttg gcagagatga atctcggcga attggccgta ggtgtcggca agctccagcc
     4141 gaatcggccg cgatgtcttg ttccagccgc gtccgatttc ggcaagcgtg tattcgaatc
     4201 cgtcgaccat gtcgatgcgc acgcggtgtt gcgccccggt caccggatta aggatcggtt
     4261 cgccacggcc ttcggtcacg ccgggcacca ccaggcgagc ctttcgcccg gcgacatcga
     4321 cctgaaactc gataggagcg aagatcggat cgtgcagttt ttcgaacgtg gacgcgaata
     4381 cgttgaagat cgtcttcccg ggctcggtgt cttgaccggt gaggatgcgc agcagcgccg
     4441 cccgctgcgg ctcggtggcg cgctcgtcga tcaccaccgc cgcttcgccc ttgccctcgt
     4501 ggatcgcacc cggccagcgg aagattccca caaacttgag gccatcgagc ttggtcgagc
     4561 cgtgatgacc ctgctcgatc tcgaagccgc cgaccgcttg gcaatgtcca tgcgtcggca
     4621 atccgttgaa ttggcagggg cagccgtagg agcagttgca attgacgaac tcacgtccct
     4681 tgatggtcca tttggtgtcc gccatggtca cctcctgacg ggaacgctcg gcgaggtcgt
     4741 atccactgag ccgtgatcga ggctgaatgc taccaagcca atgcctcatt caaaagcccg
     4801 aaagggatgt ccgctgaccc cgcaaagcga aggtgcgccg gctctcctcc actgtccgct
     4861 tcgcgccaaa aggcggcata ggcagcgcgt tcaaggagaa ggagatcaat ttcgaaccgg
//
LOCUS       DATLJT010001390         6037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1956331,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001390 DATLJT010000000
VERSION     DATLJT010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956331"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VM910_31910"
     CDS             <1..451
                     /locus_tag="VM910_31910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67146.1"
                     /translation="RQAGHFFFEPRGYDHINQATHEHKEEIKVGYNLRRKVVLMTIWA
                     LSPLVLYLDPTLFGFFSPWVTPADFVRQVAKIWLVVGIGGLLFRTVHLFFIRDVETGL
                     VWMTKIITDPFNDFKLYHKAPLALLRGELIDPGLDILDHQGLEEQHA"
     gene            complement(743..2407)
                     /locus_tag="VM910_31915"
     CDS             complement(743..2407)
                     /locus_tag="VM910_31915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI67147.1"
                     /translation="MDSSIPILSLAAGAVAATAAVAPKVKARIELSRAKHRSLAGHSK
                     MSRRMARLIPFYEFDIGDFFRSDGAPHEVATRRQDGFFRLAAQFQERFAKGRQQTSEA
                     AGQISDLQFTQAYRVPFQYSRLVRENLGAGSFMQSSAGVTLTDLDGNVLYDLTGSYGV
                     NIFGNDFYKECIEIGEKRAHALGPVLGSYHPVVAENVRRLCEISGLDEVSFHMSGTEA
                     VMQAVRLARYHTRRSHLVRFAGSYHGWWGDVQPGVGNPVSPHETYTLAEMSERTLLVL
                     RTRRDIACVLVNPLQALHPNANAPSDSALVDSSRGGGVDRAAYAEWLKRLREVCTERN
                     IVLIFDEVFVGFRLAPGGAQEYFGVRADMVTYGKSLAGGLPIGVVCGKKELMRRFRDD
                     RPADVCFARGTFNSHPYVMTAMDEFLSRLASPNFRIVYHGLDDIWNARAQKLNEMMEA
                     ADLPVRFVNLSSIWTVTYTIPSRYNWMLQYHLRAEGIALSWVGTGRLIFSLNYTDADF
                     AEVATRFVAAASRMKQDGWWWHTPALTNKGIRRQILKEMVASRFGR"
     gene            2442..3311
                     /gene="asd"
                     /locus_tag="VM910_31920"
     CDS             2442..3311
                     /gene="asd"
                     /locus_tag="VM910_31920"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797854.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVI67148.1"
                     /translation="MTVKSLIAAFTQQEDLNFLLTNRVPRAALTRFMGWFSKVENPLV
                     RDVSIALWKLFSDLDLSEARKTHFSSLHDCFTRELKAGLRPVDPDPAVVVSPSDAIIG
                     AFGTVRDGELYQIKGAPYSLSDLLGDPALVAAHRNGKFLTLRLTSSMYHRFHSPHDAH
                     IRRVTFVHGDTWNVNPIALKRIEKLFCKNERAVIETRLNNGDALTLVPVAAILVASIR
                     LHFLDVVLNAATRGPTVFPCDVQVKKGDELGWFEHGSTIILLAPEHYGFCDIVKEGAR
                     IRAGQALLRMATA"
     gene            3385..4077
                     /locus_tag="VM910_31925"
     CDS             3385..4077
                     /locus_tag="VM910_31925"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI67149.1"
                     /translation="MARLPVLAAGGIVLRREQPPRIGVVRLRKRDEWVLPKGKLDDGE
                     TARAAAWREVVEETGHNVTVHEFLGTLVYESGGRSKVVHYWRMEAGFEQAHELMNDVK
                     AVDWLPLDAAVERLSRGHERTFLENVGPLAIAALSRRLKARASAVKPPAIKAPAARRR
                     RDRVIVAPPPVFVAPEPQPLQPDLVLAATATDEAPRATGEPVDVADVQRAVEQGRRSF
                     AQKLRDWLGLAA"
     gene            complement(4099..5298)
                     /locus_tag="VM910_31930"
     CDS             complement(4099..5298)
                     /locus_tag="VM910_31930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HVI67150.1"
                     /translation="MVASYRLLLAMPLLGCLLLGGTGRVDAMLPSDQARLAPLLTDAS
                     PERTEFAQAQPAPSPTPAPQATPAPATSPAPATPDDPIGNVATLTGTATVIRNKDSIA
                     LKLKDDIFFNDTVQTSSGASLGITFNDATTFHLSENARVTIDSYVYEEGGSKNSGIFG
                     IGKGTVAFVAAALAKTGNMQINTPTATLGIRGTTGLVEVPEGAAATSANNVNIKLYPD
                     ADGRVGRIEVNDRQGARLGTLTQGASGFAIRTGAAGARAAAVPLTISPQQAARDQGIV
                     RQVHATQTVGRQIVTEQRAIRRANPPGAQPQQRPPGQPGRQQPGTTRPGQQQPGAPKG
                     PAQPGTPKGEKKTQDTPKGEKQTQGTPQTPAQPAMQPRPGGQQPGAQRPQQQRPKPPP
                     PPKKKKR"
     gene            complement(5605..>6037)
                     /locus_tag="VM910_31935"
     CDS             complement(5605..>6037)
                     /locus_tag="VM910_31935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HVI67151.1"
                     /translation="VPPAPPAAAPAPAPVTSYAPPAIAPAPAPQTPQVATVAPAPQAA
                     PVAAPTTPIGVWATEENKGNVRIELCGPNLCGYAEGSGEKVLINMKPSENNKWVGRIH
                     DPDGGGNYDSTIAMKGPNILKVQGCAFGGMFCGGQTWKRIS"
BASE COUNT         1019 a         1902 c         2004 g         1112 t
ORIGIN      
        1 gcgccaggcc gggcacttct tcttcgagcc gcgcggctac gaccacatca accaggcgac
       61 ccacgagcac aaggaagaga tcaaggtcgg ctacaatctg cgccgcaagg tggtcctgat
      121 gacgatctgg gcgctgtcgc cgctcgtgct ctacctcgat ccgaccctgt ttggcttctt
      181 cagcccctgg gtgacgccgg ccgacttcgt ccgtcaggtc gccaagatct ggcttgtggt
      241 gggcatcggc ggcctgctgt tccgcaccgt gcatctcttc ttcatccgcg acgtcgagac
      301 ggggctggtc tggatgacca agatcatcac cgatcccttc aacgacttca aactctacca
      361 caaggcaccg ctggcgctct tgcgcggcga gctgatcgat ccgggtctcg acatcctcga
      421 tcaccagggg ctggaagagc agcacgcctg agctttcgca atcgcaggcc tgaacgcacg
      481 tcatgcccgg ccttgtgccg ggcatcgacg tttttggaac atgatgatgt tgattgaggc
      541 tacggcaacg acggaagcct taccctcccc tggaggggga gggtcggtcc gcaatcgagc
      601 gaagcgagat aaggaccggg gtggggtggc agtcttgccg cgaccggacg tttgcgagcg
      661 gagggatcac cccaccccgg ctcatatttc gctgtgctca tgtgagccga ccctttccct
      721 ccaggggagg gtggcagcat ggttagcgcc cgaaccggct cgcaaccatc tctttgagaa
      781 tctgccggcg gatgcccttg ttggtgagcg ccggcgtgtg ccaccaccag ccgtcctgct
      841 tcatcctgct cgcagccgcg acaaagcgtg ttgcaacctc ggcgaaatcc gcatcggtgt
      901 agttcaggct gaagatcagc cgtccggtgc cgacccagct cagcgcaatc ccctcggcgc
      961 gcaggtggta ttgcagcatc cagttgtagc gggacgggat ggtgtaggtg acggtccaga
     1021 tcgaggacag gttgacaaag cgcaccggca ggtccgccgc ctccatcatc tcgttgagtt
     1081 tctgcgcgcg ggcgttccag atgtcgtcga gcccgtgata gacgatgcgg aaattcgggc
     1141 tggcgaggcg gctcaaaaac tcgtccatcg ccgtcatcac gtagggatgc gagttgaagg
     1201 tgccgcgcgc aaagcagaca tcggccggtc ggtcgtcgcg gaaacggcgc atcagctcct
     1261 ttttcccgca gaccacgccg atcggcaggc cgccggcaag gcttttgccg taggtgacca
     1321 tgtcggcgcg gacgccgaaa tactcctggg caccgccggg cgcgaggcga aagccgacga
     1381 acacctcgtc gaagatcaaa acgatatttc gctcggtgca gacctcgcgc agcctcttca
     1441 gccattcggc gtaggccgcg cggtcgacgc cgccgccgcg ggaactgtcg accagcgcgg
     1501 aatcgctcgg cgcattggcg ttcggatgca gcgcctggag cggattgacc aggacgcagg
     1561 cgatatcgcg ccgcgtccgc aacacaagca gcgtgcgctc gctcatctcg gccagcgtat
     1621 aggtctcgtg cggcgatacc ggattgccga cgcccggctg cacgtcgccc caccagccgt
     1681 gatagctgcc ggcgaagcga accagatgcg agcggcgcgt gtgatagcgc gcaagccgca
     1741 ccgcctgcat cacggcttcg gtgccggaca tgtggaagga gacttcgtcg agcccggaga
     1801 tttcgcaaag acgccgcaca ttctcggcca cgaccgggtg ataggagccg agcacggggc
     1861 cgagcgcatg cgcgcgcttc tcgccgatct cgatgcactc cttgtagaag tcgttgccga
     1921 agatgttgac gccgtaggag ccggtcaggt cgtagaggac gttgccgtcg aggtcggtca
     1981 gcgtcacccc cgcggacgat tgcatgaacg aaccggctcc gagattctcg cggaccagcc
     2041 ggctgtactg gaacggcacg cggtaggctt gcgtgaattg caggtcggag atctggcctg
     2101 ccgcctccga ggtctgctgc cgtcccttgg caaaacgctc ctggaattgg gccgcgaggc
     2161 ggaagaaacc gtcctgccgc cgtgtcgcta cctcgtgcgg cgcgccatcg gagcggaaga
     2221 aatcgccgat gtcgaattcg tagaacggga tgagccgcgc catgcggcgc gacattttgg
     2281 agtgtccggc gagcgagcgg tgcttggccc gcgacagctc gatccgcgcc ttgaccttgg
     2341 gcgcgaccgc agccgtggcc gcgaccgctc cggcggcgag agacagaatc ggtatcgacg
     2401 aatccataac cacagcgcta accagtcctg ctgacagatt catgacagtg aaaagcctga
     2461 tcgccgcctt cacccaacag gaagacctca acttcctgtt gaccaaccgc gtcccgcgcg
     2521 cggccctgac ccgattcatg ggatggttca gcaaggtcga gaaccccctg gtgcgcgacg
     2581 tttcgatcgc gctctggaag ctgttttccg acctcgatct ctcggaagcc agaaagacgc
     2641 atttttccag cctgcatgat tgtttcacgc gcgagctcaa ggcgggcctg cgtccggtcg
     2701 atcccgatcc tgccgtcgtg gtcagccctt cggacgccat cattggcgcg ttcgggacgg
     2761 tccgcgacgg cgagctctac cagatcaagg gcgcgcccta ttcgctgtcg gacctgctcg
     2821 gcgatcccgc gctggtcgcc gcgcaccgca acggcaaatt cctgaccttg cggctgacct
     2881 cgagcatgta tcaccgcttt cacagccccc atgacgcgca tatcaggcgc gtgacgttcg
     2941 tccatggcga tacctggaac gtcaatccga tcgcgctgaa gcggatcgaa aagctgttct
     3001 gcaagaacga gcgcgccgtg atcgagaccc gcctgaacaa cggcgatgca ctgacgctgg
     3061 tgccggtcgc agccattctg gtcgccagca tccgcctgca tttcctcgat gtcgtcctca
     3121 acgccgccac gcgcgggccg accgtgtttc cctgcgatgt gcaggtaaag aagggcgacg
     3181 agctcggatg gttcgagcac ggctcgacca tcattctgct cgcgccggaa cattacggct
     3241 tttgtgacat tgtgaaggag ggcgcgcgaa tacgcgccgg ccaggccttg ttgcggatgg
     3301 cgacggcgta gattcgtttt tgcttggcac ccgtcgtgtc gcgccgctat agagaacggg
     3361 aatccatttt cagaaagacg ttcgatggct aggttgccgg ttctggcagc gggcggcatt
     3421 gtgctgcggc gggagcaacc gccgcgcatt ggcgtcgtgc ggctgcgcaa gcgtgacgaa
     3481 tgggtgctgc cgaagggcaa gctcgatgac ggagaaacgg cgcgcgcggc agcctggcgc
     3541 gaggtggtgg aagaaaccgg gcacaacgtg accgtccacg aattcctggg cacgctggtc
     3601 tatgaatccg gcggccgctc caaggtggtg cattactggc gcatggaagc cggcttcgag
     3661 caggcccacg aattgatgaa cgacgtgaaa gcggtcgact ggttgccgct cgatgcggca
     3721 gtcgaacggc tgtcgcgcgg ccacgagcgc acctttctcg agaatgtcgg tccgctcgca
     3781 atcgccgccc tgtcgcggcg gctcaaggcc agggcttcgg ccgtcaagcc gccggccatc
     3841 aaggcacccg cggccaggag gcgccgtgat cgggtgatcg tggcgccgcc gcccgttttc
     3901 gttgcgcccg agcctcagcc gctgcaaccc gacctcgtgt tggcggcgac tgccaccgac
     3961 gaggctccgc gcgcgacggg cgagccggtg gatgtcgcgg acgtccaacg cgccgtcgaa
     4021 caggggcggc ggagctttgc gcaaaagctg cgcgactggc tcggcctcgc cgcgtgagtc
     4081 tctaaacgct ctcggtggct agcgcttttt cttcttcggc ggcggaggcg gcttgggccg
     4141 ttgctgttgc ggccgttgcg cgcccggctg ctgtccgccc ggtcgcggct gcatcgcagg
     4201 ctgcgccggc gtttgcggcg tgccctgcgt ctgcttctcg cccttcggcg tatcttgcgt
     4261 ctttttctcg ccctttggcg tgccgggttg ggccggaccc ttcggcgcgc cgggttgctg
     4321 ctggccgggg cgagtcgttc cgggttgctg ccgaccgggt tgaccgggcg gtcgctgctg
     4381 cggctgtgcg ccgggcggat tggcgcggcg gatcgcgcgc tgctcggtca cgatctggcg
     4441 accgaccgtc tgcgtcgcat ggacctggcg cacaatcccc tggtcgcgcg ccgcctgctg
     4501 cggcgagatg gtgagcggca ccgcggcggc tcgcgcgcct gccgcgcccg tgcggatcgc
     4561 aaagccgctc gcgccctgtg tcagcgtgcc gagccgcgcg ccttggcggt cgttgacctc
     4621 gatgcgtccg accctgccgt cggcatccgg atagagcttg atgttgacgt tgttcgcgct
     4681 ggtggcggcg gcgccttccg gcacctcgac gaggcccgtg gtgccgcgaa tgccgagcgt
     4741 tgcggtcggc gtgttgatct gcatgttgcc ggttttcgcc agcgcagccg cgacaaaggc
     4801 aaccgtgccc ttgccgatgc cgaaaatgcc cgagttcttg ctgccgcctt cctcgtagac
     4861 gtaggagtcg atcgtaaccc tggcgttctc cgagagatgg aaggttgtcg catcgttgaa
     4921 ggtgatgccg agcgaagcgc ccgacgaggt ttgcaccgtg tcgttgaaga agatgtcgtc
     4981 cttgagtttg agcgcgatcg aatccttgtt gcggatcacg gtggcggtcc cggtcagcgt
     5041 cgcgacattg ccgatcggat cgtccggtgt cgcgggcgca ggcgaggtgg cgggcgctgg
     5101 cgtagcttgc ggtgcagggg tcggcgaggg ggcaggctgt gcctgggcaa actctgttcg
     5161 ctccggactc gcgtcggtca agagcggcgc gaggcgggct tgatccgatg ggagcatcgc
     5221 gtcgacgcgt cccgtgccgc cgagcaggag acagccgagc agcggcattg ccagaagaag
     5281 ccgataggaa gccacgatga tgccccaacg cggaaatgca gaagacctgc cgatttcagc
     5341 cgcgtcttgc tgaatgccga atgaacgggg agcgccaagt cgtcgcttgc cgctcgccat
     5401 tcacctttct tttctttcat tcacaaaacc agcagggagc aaaacgatca agcgctgcgt
     5461 ccccttgatc aacaggacac gtgatcaaca ggacacggcg ctagagcaaa gcgtcggcag
     5521 ttgaagcctc agccgcgctc gcaaaagcgc gcgctttgca gaacgaccgc aaagcgcgcg
     5581 tgaacttgaa cgagcaacgc taaattagct gatgcgcttc caggtctgac cgccgcagaa
     5641 catgccgccg aaggcgcaac cctggacctt gagaatgttc ggtcccttca tcgcaatcgt
     5701 cgaatcgtaa ttgccgccgc cgtccggatc gtgaatccgg ccgacccatt tgttgttctc
     5761 cgacggcttc atgttgatga ggactttttc gccgctgcct tcggcatagc cgcagagatt
     5821 ggggccgcac agctcgatcc gcacattgcc cttgttctct tcggtcgccc aaacgccgat
     5881 cggcgtggtc ggggcggcaa cgggagcagc ttgcggcgca ggcgcaacgg ttgcgacctg
     5941 cggggtttgc ggggcgggag cgggagcaat ggccggtggg gcgtagctgg tgaccggtgc
     6001 gggcgcagga gcagcagcgg gcggcgccgg cggaacg
//
LOCUS       DATLJT010001391         1892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4253458,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001391 DATLJT010000000
VERSION     DATLJT010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4253458"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /gene="thyA"
                     /locus_tag="VM910_31940"
     CDS             complement(<1..312)
                     /gene="thyA"
                     /locus_tag="VM910_31940"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HVI67152.1"
                     /translation="MNQYHDLLERILADGVEKHDRTGTGTLSIFGHQMRFPLSAGFPM
                     LTTKRLPLKAIVHELLWFLKGDTNIKYLKDNGVTIWDEWADENGNLGPVYGSQWRSWP
                     AP"
     gene            complement(558..>1892)
                     /locus_tag="VM910_31945"
     CDS             complement(558..>1892)
                     /locus_tag="VM910_31945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI67153.1"
                     /translation="LIMLAGIYYGAQYGGSTTSILVNIPGEATSVVTTLDGFQMAKQG
                     RAGPALAIAAIGSFIAGCAATVLIAVLGAPLTKIALAFGPAEYFSLMVLGLIFAVVLA
                     KGSVLKAIAMIVFGLLLSMVGSDIETGASRMAFNIPELADGLEFATVAMGVFGFAEII
                     RNLDTGAEMDRELVQQKITGLMPTKKDMLASLPAIFRGTVLGSILGILPGGGAVIASF
                     AAYTFEKKVARDPKRFGRGAIEGVAAPESANNAAAQTSFIPLLTLGIPPNAVMALMVG
                     AMTIHGIVPGPQVMQKQPDLVWGMIASMWIGNLMLIIINLPLVGIWVRLLRVPYRLMF
                     PSIVVFCAIGIYSINNAPMDVILAGIFGLIGYWLVKHDFEPAPLLLGMVLGRLMEENL
                     RRAMLISRGDWTVFITRPLSAVLMAAAAALLVLAVLPTLRKKRDEVFAESEG"
BASE COUNT          398 a          601 c          574 g          319 t
ORIGIN      
        1 cggcgcgggc caggaccgcc actgcgagcc atagaccggg ccgagattgc cgttctcgtc
       61 agcccactcg tcccagatcg tgacgccgtt gtccttcaga tatttgatgt tggtgtcgcc
      121 tttgaggaac cacaacagct cgtgcacgat cgccttcagc ggcaggcgct tcgtggtcag
      181 catcgggaag ccggccgaaa gcggaaaccg catctgatga ccgaagatcg acaacgtgcc
      241 ggtgcctgtg cggtcgtgct tctccacccc gtcggcgagg atgcgttcga gcaggtcgtg
      301 atactggttc attgcgtcgc gcccgttgaa agaaggcgta tttaacggcc ggaggcgctg
      361 atcgacacgc cgattcggcc tgataaccca attatacccg gaaaaatgtg ttgatccccg
      421 ctaaagcgca atgcgcttag ctattgttta cgcaggatct tttcggaaaa accggttccc
      481 gcttttccca gtcatgccga aaacgacggg gagcggaacc cgtgttccct ccccgccccg
      541 aagcgccgca agccagctca gccctcggat tccgcaaaca cctcgtcgcg ctttttgcgc
      601 aaggtcggca gcacggcgag caccaggagc gcagccgcag ccgccatcaa cacggccgac
      661 agcggacggg tgatgaacac cgtccagtcg ccccgcgaga tcaacatggc gcgccgcagg
      721 ttttcctcca tcagccgccc aagcaccatc ccgagcaaaa gcggcgccgg ttcgaaatcg
      781 tgcttgacca gccagtagcc gatcaggccg aagatgccgg cgaggatgac gtccatcggt
      841 gcgttattga ttgagtaaat accgatcgcg cagaacacca cgatcgaggg aaacatcagc
      901 cggtacggca cccgcaacag acgcacccag atgccgacca gcggcaggtt gatgatgatc
      961 agcatcaggt tgccgatcca catcgaggcg atcatgcccc agaccagatc aggctgcttc
     1021 tgcatcacct gcggccccgg cacgatgcca tgaatggtca tcgcgccgac catcagcgcc
     1081 atcaccgcat tcggcgggat gccgagcgtg agcagcggaa tgaacgaggt ctgcgccgcc
     1141 gcgttattgg cgctttcggg tgccgccacg ccttcgatcg cgccgcgccc gaaccgcttc
     1201 ggatcgcgtg cgaccttctt ttcgaatgtg taggcggcaa aggaggcaat gaccgcgccg
     1261 ccacccggca gaatgccaag gatcgatccg agcacggtgc cgcggaagat cgccggcagc
     1321 gaggccagca tgtccttctt ggtcggcatc agcccggtga tcttctgctg aaccagctcg
     1381 cgatccattt cggcgccggt gtcgagattg cgaatgatct cggcaaagcc gaacacgccc
     1441 atcgccacgg tggcgaactc gagaccgtcg gcgagttcag ggatattgaa ggccatgcgc
     1501 gaggcgccgg tctcgatgtc ggaaccgacc atcgacagca acagcccgaa cacgatcatc
     1561 gcgatagcct tgagcacgct gcccttggcg agcaccacgg cgaagatcag gccgagcacc
     1621 atcagcgaga aatattcggc cgggccgaag gcgagcgcga tcttggtcaa gggcgcgcca
     1681 agcacggcaa tcaggacggt cgccgcgcag ccggcaatga acgagccgat cgctgcgatt
     1741 gcgagcgcag gcccggcgcg gccttgcttg gccatctgga agccatcgag cgtggtgacg
     1801 acggaggttg cctcaccggg aatattgacc aggatcgagg tggtcgatcc gccgtattgc
     1861 gcgccgtaat aaatgccggc gagcatgatc aa
//
LOCUS       DATLJT010001392         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_805613,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001392 DATLJT010000000
VERSION     DATLJT010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805613"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1634
                     /locus_tag="VM910_31950"
     CDS             480..1634
                     /locus_tag="VM910_31950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67154.1"
                     /translation="MFAGAAFSAAAGLSITSADTQTTPRTTIPPLTLAEHGYFFVGGQ
                     YVESGGKRLMSGQMYVEYLTPQNVTRPYPIIMIHGTAQTGTNFMGTPDGRPGWAHNFL
                     TRGYRVYVVDQVGRARSGADPQIYGPYMRFAVEQEESLFTAPEKYKLWPQATLHTQWP
                     GGPGVAGNPAFDQFFASQLESIASTVKTEELMFPADVALLEKIGPAIVLTHSQSGVFG
                     FKLADARPDLVKAHIAVEPNGPPFYDTDFRGGDDWYKDSDKIARPWGVARVPLAYDPP
                     AAEASELHLARQEQADETTLVRCWLQAEPARQLPKMKNVPTAIVTGEASFRATYDHCT
                     SKFLTQAGVRNTHLRLEKVGLHGNGHMMMLEQNSATIAEVIANWLDQNMK"
     gene            1804..2055
                     /locus_tag="VM910_31955"
     CDS             1804..2055
                     /locus_tag="VM910_31955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67155.1"
                     /translation="MKTVAMIFTLALVGSQALAQEQTRIYGPDGRSLGTAAPYGTGSV
                     RYRDSRGRTTFTTTTMNGVTRFYDSGGHLTATVIGSRRR"
     gene            2162..>2394
                     /locus_tag="VM910_31960"
     CDS             2162..>2394
                     /locus_tag="VM910_31960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67156.1"
                     /translation="MTVTIGRRQLLVALGGAAVAWPLAARAQQLAKVPTIGFLGSGTP
                     MTAGAWVVAFAQRLRELGWIEDRTIKIDLRWAEG"
BASE COUNT          472 a          723 c          748 g          451 t
ORIGIN      
        1 ttctggagct tgcgagagag caatgacgcg tgatggcaca gtcggtcgat ccccgacgca
       61 accgagccaa acctggcgtt tcatcgggac atcgatcccg tttgaatgat gaggactcgg
      121 ttcgcggacc ccatcagggc cggcagtcca cccggactgc accaacaggc cggacacatg
      181 actgcaagcg acctctgcgc tgcacgccaa atgatctctt gccaggcggg ggccatccac
      241 acatgaaaag gcgcgagttc atcacgctgc tcggcggcgc cactgtagcg tggccttgca
      301 acggattagc tcagccagcg cctaagcggc cgctctgctt attcatggta ttatggcgta
      361 agcacgggcg tgcgcgctga atggcttgga ccagttaaga ttaatcagtc ggagcacggc
      421 atgctgggcg tgcagacagg gaggaagaca tgaaaactcg gtgtgtaata gcattattgg
      481 tgtttgcagg cgccgcgttt agtgctgctg ccggactatc aatcaccagc gcggatactc
      541 aaacgacacc gagaaccacc atcccgcccc tcacgctcgc tgagcacggt tacttttttg
      601 tcggcgggca gtatgtggag agcgggggca aacgcctcat gtccgggcag atgtatgtgg
      661 agtatctgac gccccaaaac gtgacgcgtc cctatcctat catcatgatc cacggaaccg
      721 ctcaaaccgg taccaatttc atgggcacgc ccgatggccg gcccggttgg gcgcacaact
      781 tccttacccg aggctatcga gtctacgttg tggatcaggt ggggcgcgcg aggtcaggtg
      841 ccgaccccca gatctatggt ccgtatatgc gcttcgcagt ggaacaggag gagtcgctgt
      901 tcactgcgcc agagaagtac aaactttggc cacaagccac tttgcacacg cagtggccag
      961 gcggccccgg cgtcgctggc aatcctgctt tcgaccagtt cttcgcctca caactcgaga
     1021 gcattgcgag cacggtcaag accgaggagc tgatgtttcc cgctgatgtt gcactgctcg
     1081 aaaagatcgg ccctgcaatc gtgctcaccc attcacagtc cggcgtcttc ggcttcaagc
     1141 tcgctgatgc gcgccctgac ctcgtgaagg ctcatatagc cgtcgagccg aacggcccgc
     1201 cgttctacga cactgatttc aggggtggtg atgattggta caaggacagc gacaaaattg
     1261 ctcgtccctg gggcgtggct cgtgttccct tggcttacga tccgccagcc gccgaggctt
     1321 cggaactcca cttggcgcgg caggaacagg cagacgaaac gacactggtt cggtgctggc
     1381 ttcaagcgga gcccgcgcgg caattgccga agatgaagaa cgtgccgacc gcgatcgtga
     1441 ccggcgaagc gtcgttccgc gcgacttacg atcactgcac gtcgaagttc ctaacccaag
     1501 ccggcgtacg gaacacgcac cttcggttgg agaaagtcgg tctacacggc aatggacata
     1561 tgatgatgct ggaacagaat agcgcgacta tcgctgaggt aattgcgaac tggctcgacc
     1621 agaatatgaa gtaggttggg cgggatgccg ttgcctgctg cgtttcgttt tgcttcggcg
     1681 catgagtccg agtttggcac ttctcggacc tcgcgagatg tccgacttga gtccgcaaag
     1741 tgggctaaag cggaccttgg atcaggtcgc tgtggtggtg cccatccgca agcgaggaga
     1801 gcaatgaaga ccgtagcgat gatcttcacc ctggccctcg tcggctccca ggcgctcgcc
     1861 caggagcaga cccggattta cgggccggat ggccggagcc tcggcacggc cgcgccctac
     1921 ggcactggca gcgtccggta ccgcgactcg cgcggccgca ccacgttcac gacgacgacg
     1981 atgaacggtg tgacgcggtt ctacgactcc ggcggccacc tcacggccac ggtcattgga
     2041 tcgcgtcgcc gctgatggcc aggtcgagtc gcccctgccc gccgggggac gcgccgttat
     2101 aggacagcgc gctcgtggcg tggtatcgtt acatttacgc accctcgcac gggaggggcg
     2161 catgacagtc accatcggac ggcggcaatt gctggtcgca ctcggcggcg cggctgtggc
     2221 gtggccgctc gcagcgcggg cgcagcagct ggcgaaggtg ccgaccatcg gcttcctagg
     2281 ctcgggcacg cccatgacgg caggggcatg ggtcgtcgcc tttgcgcagc ggctccgcga
     2341 actcggctgg atcgaggacc gcaccatcaa gatcgacctg cgctgggcag aagg
//
LOCUS       DATLJT010001393         2076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4025974,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001393 DATLJT010000000
VERSION     DATLJT010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4025974"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VM910_31965"
     CDS             <1..1272
                     /locus_tag="VM910_31965"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVI67157.1"
                     /translation="VMLYGGSIASGHTVGQGGMFCGRNLTIQDIYEALGAYNAGKITE
                     SELKDVEDHACPGAGACGGQFTANTMATAYQMLGISAMGSNDIPALDPLKADAARESG
                     RLAVKLVNEGVTPRSLVTRRSFENAITGVLATGGSTNAVLHLLATANDFGIPLSIDDF
                     DRLSRRTPVLADLRPWGTYTAPEMYAAGGMAVVGKRLLEAGLLHPEEKTVTGRSLGEE
                     IVRAREPKGQDVIKRLDHALKKEGGIAILRGNLAPGGCVMKVSGQTKRVHRGAARVFE
                     REEDAFIAIKEGRIQPNDVMVLRNEGPKGGPGMREMQLVTGALQGAGLGESVALITDG
                     RFSGATRGFVIGHIVPEAVERGPIAALVDGDTITIDVERRRLDVALSDTQVSDRLSKW
                     RAPRSPYLRGVLAKYARLVTDASHGAITDLT"
     gene            complement(1356..>2076)
                     /locus_tag="VM910_31970"
     CDS             complement(1356..>2076)
                     /locus_tag="VM910_31970"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI67158.1"
                     /translation="DTKFVPVYATGINPDQPEEFAVARYREGHRAFKLKIGFGREIDV
                     RNLANMRKALGNDAKLMCDANQALSLAAAIALSREIAPSNLSWLEEPLRVDAPADDWQ
                     ALASASPIELAGGENFQGDQFDGAVASHVYGVLQPDVTKWGGITGNIRVARAAVAAGK
                     RYCPHYFGGGIALLASLHLLAAAGGEGLLEFDCHPNAGREIVVGPLLPVHEGRVPVPT
                     TPGLGSIPDLRAFARYRTWPQ"
BASE COUNT          391 a          632 c          673 g          380 t
ORIGIN      
        1 gtgatgctgt acggaggctc gatcgcatcc ggccataccg tggggcaggg cgggatgttc
       61 tgcggccgta acctcaccat tcaggacatc tacgaggcgc tcggcgccta caacgccggc
      121 aagatcacgg agtcggagtt gaaggatgtg gaggatcacg cttgccccgg agccggggcc
      181 tgcggaggtc agttcacagc caataccatg gccaccgcct atcagatgct cggcatctcg
      241 gccatgggct cgaacgatat tccggccctc gatcctctga aggcggacgc cgcacgcgag
      301 agtggccggc ttgccgtaaa gctcgtcaac gaaggcgtga ccccgcgctc tcttgttacg
      361 cgacggagtt tcgaaaatgc aatcaccggc gtgctggcga cgggcggatc cacgaatgcc
      421 gtccttcacc ttctggcgac ggccaacgat ttcggcatcc cgctttcgat cgacgacttt
      481 gaccgcttgt ctcgtcgcac gccggtgctt gcggatttgc ggccatgggg gacgtatacg
      541 gcgcccgaga tgtatgcagc cggcggaatg gcggtcgtcg gcaagcggct ccttgaagcc
      601 ggtctgcttc atccggaaga gaagaccgtg acaggacgca gcctggggga ggagatcgtg
      661 cgagcgcgcg agcccaaggg gcaggatgtc atcaaacgac tcgatcatgc gctgaagaag
      721 gagggcggaa ttgccattct tcgcggcaat ctcgctcccg gcggctgcgt catgaaggtc
      781 tccggtcaga cgaagcgcgt gcaccgtggc gctgcgcgag tgttcgagcg cgaggaagac
      841 gcattcattg cgatcaagga aggtcgcatc cagccgaacg acgtcatggt gctgcgtaac
      901 gaaggcccca agggaggccc cggtatgcgc gagatgcaat tggtgaccgg cgctcttcag
      961 ggcgcggggc tcggcgaaag cgtggccctg atcaccgacg gccggttctc tggcgcaacg
     1021 cgcggcttcg tcatcggaca catcgtgccc gaagcggtcg agcgaggtcc gattgccgcc
     1081 ctggtggacg gcgacaccat cacaatagac gtggaacggc ggcgactgga cgtcgctttg
     1141 agcgacaccc aggtatcgga tcggctcagc aaatggcgag caccaagatc accttatttg
     1201 cgtggcgtcc tggccaaata tgcgcgactg gtgacagatg cctcgcacgg agccatcacg
     1261 gacctgacct gagcgttttg cgcagacctt gtgcaggaat aggcttagcc aagaagcttc
     1321 tcgccgatct cagtcatttg cttcgccggc aaggcctatt gcggccatgt acgatagcgc
     1381 gcgaacgcgc ggagatctgg gatgctgccg agccctgggg tggttggcac tggcacgcgt
     1441 ccttcgtgga ccggcagcaa aggaccaacc acgatttcgc ggccagcgtt gggatgacaa
     1501 tcgaactcga gcaggccttc tccgcctgct gcggcgagca gatgcaacga tgccagcagc
     1561 gcgataccgc cgccgaaata gtgcggacaa tagcgcttgc ctgccgccac cgccgctcgt
     1621 gctacgcgga tgttgccggt aatgccgccc cacttggtga cgtctggctg cagaacgccg
     1681 tacacgtggc tggcgaccgc gccatcgaac tggtcgcctt gaaagttctc gccgccggcg
     1741 agttcgatcg gcgaagcgct tgcaagcgcc tgccaatcgt ccgccggagc atctacccgc
     1801 aacggctctt ccagccagga taggttgctc ggtgcgatct cgcggctgag cgcaatggcg
     1861 gctgcaagag acagcgcctg gttggcatcg cacatcagct ttgcgtcgtt gcccagggcc
     1921 ttgcgcatat tggcgagatt cctgacgtcg atttcccgcc caaagccaat cttcagcttg
     1981 aaggcccggt gcccctcgcg gtaacgcgcc acggcaaact cctcgggttg atccgggttt
     2041 atgccggtcg cgtagaccgg gacgaatttg gtgtct
//
LOCUS       DATLJT010001394         1924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_805648,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001394 DATLJT010000000
VERSION     DATLJT010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805648"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1450)
                     /locus_tag="VM910_31975"
     CDS             complement(149..1450)
                     /locus_tag="VM910_31975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HVI67159.1"
                     /translation="MSVTARRVRPAPASDPKQPRPMRVLGPGLITGASDDDPSGIATY
                     SQAGAQLGFAISWIMLFTYPLIASIQQISARIGRTTGKGIAGNLRQHYPNWLLQGIVA
                     LLFVANTINIGADLGAMADALGLLIGGPSLIYVIAFGALCAILQVFMAYARYVAMLKW
                     LTLALFAYFGTVMVVDIPWAEAAHGFLIPTFLPDAAFWTTVVAVLGTTISPYLFFWQA
                     AQEVEDIRAEPERRPLVKAPHQGADAIQRIRLDTYVGMAFANLVALAIIVTTAATLHA
                     DGITDISTSSEAAQALRPVAGPFAFTLFALGIVGTGLLAVPVLAGSAAYAIGEARRWP
                     VGLNHKPQKAKAFYATLTIATMVGAALNFSPINPIKALYWSAVINGVVAVPVMVAMMH
                     MTANPKVMGKFPVHDGLRLVGWMATAVMAAAAVAMGVTAIV"
BASE COUNT          365 a          613 c          614 g          332 t
ORIGIN      
        1 ccttgccggc gacctccttg accttgccct tggcctgttg agccgaaccc ttgatgcgat
       61 ccttatccat tgcatgtctc cggtcgttgt gacgggtgaa cggcgatgcc gccgacaagg
      121 ttccgcgcgc agctaccagc cacgactgtc agacaatggc agtcacgccc atcgcgactg
      181 ctgccgccgc catgaccgcc gtcgccatcc aaccgaccag gcgcaggccg tcatggacgg
      241 gaaatttccc catgaccttc ggattggcgg tcatgtgcat catggcgacc atcaccggca
      301 ccgccaccac gccgttgatc accgcgctcc aatagagcgc cttgatcggg ttgatcgggg
      361 aaaagttgag tgcggccccc accatggtcg cgatcgtcag cgtcgcatag aacgccttgg
      421 ccttttgcgg cttgtggttg agaccgaccg gccatcggcg cgcctcgccg atggcgtagg
      481 cggccgatcc cgccaagacc gggaccgcga gcaggccggt gccgacgatg ccgagggcga
      541 acagcgtgaa cgcaaacggc ccggccacgg gccgcagcgc ctgcgctgct tcgctcgagg
      601 tcgagatgtc ggttattccg tcggcgtgga gcgtcgccgc ggtggtgacg atgatcgcga
      661 gcgccaccag gttggcgaac gccattccga cgtaagtgtc cagccggatt cgctggatcg
      721 cgtccgcccc ctggtgcggc gccttcacga gcggcctgcg ttcgggctcg gcccgaatat
      781 cctcgacctc ctgcgccgcc tgccagaaaa acaggtacgg gctgatggtg gtccccagca
      841 ccgcgaccac ggtcgtccag aacgcggcgt ctggcaggaa ggtcgggatc agaaaaccgt
      901 gcgccgcctc ggcccaggga atatccacga ccatcaccgt gccgaaataa gcaaacaagg
      961 caagcgtgag ccatttcagc atggcgacgt agcgggcata ggccatgaac acctgtagga
     1021 tcgcgcacaa cgcgccgaac gcgatcacgt agatcaagga aggtccgccg atcagcaatc
     1081 cgagcgcatc ggccatcgca ccgaggtcgg cgccgatgtt gatcgtgttg gcgacgaaaa
     1141 gaagcgcaac aatcccttgc agcaaccagt tgggatagtg ctggcgcaga ttgccggcga
     1201 tccctttccc cgttgtacga ccgatccttg cgctgatctg ctggatcgac gcgatcagtg
     1261 ggtaggtgaa cagcatgatc caagagatcg cgaaaccgag ttgggcgccg gcctggctgt
     1321 aggtcgcgat tccgctgggg tcgtcgtcgg aggctccggt gatgaggccc gggccgagca
     1381 cgcgcatggg ccgcggctgt ttcggatcgg acgcgggcgc cggacggacc cgccttgctg
     1441 tgacggacat gcaatgtttt cagcgccgct cggcgagaac gcgtggacgg agctcggcgc
     1501 cgtccctgat gacgagcaac gtacaagccg aggctaaagt tccttgcatc ccgcaatccg
     1561 aatcggtcgg cctgtgccga gggtaaacgt cgccgcgtct cccccgcgcg cgtcgaggtt
     1621 aacagccgct tcgtcgcgcc gaattcacgc aggcgtcggc ggcgatgagg cgcgcggcgc
     1681 cggccaatgg cctgacggtt gagcttttgc cattcggtct gcgcgagccg ttcttcccgc
     1741 aacgccgctg agacgcacga ggcaaatgag aaaccgtcgg gaacataccc acgctaaaaa
     1801 cgttctctcg ctgtggaatc ctaccggagg agcaaacgcc gctgcttggc gtttcggtta
     1861 ttccgtcggg aggcggatca ttcacaccat gctgaagaag aagtgtgaga tcgcagagaa
     1921 aatc
//
LOCUS       DATLJT010001395         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1268867,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001395 DATLJT010000000
VERSION     DATLJT010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268867"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VM910_31980"
     CDS             <1..400
                     /locus_tag="VM910_31980"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI67160.1"
                     /translation="QGHSATGLALMYAGRAAEALEAFAVAKRLDPHYASIVLHFMAQT
                     HFSLGQYETATKQLLERIARTPATDSSRMMLASCYGHLGRADDARAAWAELLKVNPGF
                     SLAQRERVLPYKNAADFQRIVEGLAKAGLP"
     gene            491..874
                     /locus_tag="VM910_31985"
     CDS             491..874
                     /locus_tag="VM910_31985"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI67161.1"
                     /translation="MKTCYALALAALTGVGIGGLAVQGIHAQAKPPAYHIAEIDVTNE
                     DLYNKEWAPKADATVRAAGGIYLVRGTNIERIEGAAPKRLLITKWDNIDKLTAWRESE
                     AYMKTLPILDKAVKSVRSYAVEGVN"
     gene            1119..1364
                     /locus_tag="VM910_31990"
     CDS             1119..1364
                     /locus_tag="VM910_31990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67162.1"
                     /translation="MPDNVSASTRYRRLARECLEVAHTFPGGERRTVLLQMAQVWQRL
                     ADEYADTSAPLLRPIESEQPTMQQQQQIQPKDDDKQG"
     gene            1494..1901
                     /locus_tag="VM910_31995"
     CDS             1494..1901
                     /locus_tag="VM910_31995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HVI67163.1"
                     /translation="MLRDDSGHGGGRPNTPRNVLGDRLEVCSINPMTGFFRDGCCDTE
                     QQDIGSHTVCAVMTAAFLAFSKSRGNDLSTPVPEFGFRGLKPGDRWCLCAPRWQEALE
                     AGQAPRVVLRATHEGALDYCSLADLKRFAVDLA"
     gene            1913..2569
                     /locus_tag="VM910_32000"
     CDS             1913..2569
                     /locus_tag="VM910_32000"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI67164.1"
                     /translation="MAVAYVLLGLVVVIGLVAVIWRVGSRRHSVPCPVWLRWLVELDS
                     PFTKTNRAAVIVERLDLQPGMAVLDAGCGPGRLAIPIARHVGPRGEAVAMDIQAGMLE
                     RAREKAAAANLANIRFLEGGLGEGKLGRNRFDRSVLVTVLGEVPDREAALREIFDALK
                     PGGILSVTEIIFDPHFQSRPTVARLARSVGFREQAFHGNRVAFTLNLEKPYDAAPQRA
                     "
BASE COUNT          509 a          864 c          890 g          464 t
ORIGIN      
        1 ccagggccat tcggcgacgg gactggcact gatgtatgca ggccgcgcgg ccgaggcgct
       61 cgaggcgttc gcggtcgcga agcggctcga tccgcactat gcgtccatcg tgctgcactt
      121 catggcgcag acgcatttca gtctgggaca atacgagacc gccacgaagc aactcctcga
      181 gcgcatcgcg cgcacccccg ccaccgattc gagccgcatg atgctggcgt catgctacgg
      241 ccatctcggg cgcgcggacg acgcgcgcgc ggcatgggcg gagcttctca aggtaaatcc
      301 aggcttctcg ctggcgcagc gcgagcgcgt cctgccgtac aagaacgccg ctgattttca
      361 gcgcatcgtc gaaggcctgg cgaaggccgg attgccgtag gcgcgcgtat tgctgcccaa
      421 tcgccgcccg aaaaaatttg agcgaggttt cgcgatcaaa cgtccagccc ccacagcgag
      481 gatcaatctc atgaaaacat gctacgcgct ggcgttggcc gcgctcacgg gcgtcggtat
      541 cggcggcctg gccgtccaag gcattcacgc gcaagccaag ccgccggcat atcatatcgc
      601 cgaaatcgac gtgaccaacg aagaccttta caacaaagag tgggccccaa aagccgacgc
      661 gacggtcagg gccgcagggg gcatctacct cgtgcgcggc acgaacatcg aaaggatcga
      721 aggagctgcg ccaaagcggt tgttgatcac caaatgggat aacatcgaca agctgacggc
      781 ttggcgtgaa tccgaggcgt atatgaagac gctgccgatc ctcgataagg cggtcaaatc
      841 cgtgcgctca tacgcggtcg aaggcgtaaa ctagcggctg tggcgcttcg cgcggcgttc
      901 gccgggcatg ttatgccggc cgcccgactt tggaaccgct gctcggacca gccgtttgcg
      961 cacgcgacca gcatcccgga gggaaccacg ggtttgcccg caggcttaat gccgtggcaa
     1021 ggtgccgatc atgcccaacg gatagcgggg gccggcacca cggacctttt gataaggcgc
     1081 atctatcgtg cgagcactgg taaaggagta gctcagccat gccggacaat gtctctgcga
     1141 gcacccgcta ccgtcgcctt gcgcgagaat gtctggaggt cgcgcatacc tttccgggcg
     1201 gggaacgtcg gaccgtcctg ctgcagatgg cgcaagtctg gcagcggctg gcggacgagt
     1261 acgcggacac cagcgcgcct ctgctgcggc cgatcgagag cgagcagccg accatgcagc
     1321 agcaacagca gattcagccc aaggacgacg acaagcaagg ataagccgcc gcagatgacg
     1381 gccgcccccg gtccgggcac gggatggcgc tgacccggca aaatgccaga gagtgtggac
     1441 gggttgcctt ttccggagta tcgcggaaca cgttcacgcg cgcggagacg gccatgctga
     1501 gggacgacag cggtcatggc ggcggtcggc cgaacacgcc ccgcaacgtg ctcggtgatc
     1561 ggctcgaagt gtgctcgata aatcccatga cggggttctt tcgcgacggc tgctgcgata
     1621 cggagcaaca agacatcggc agccacacgg tctgcgccgt catgaccgcg gcgtttctcg
     1681 cgttctccaa atcccgcggc aacgaccttt caacgccggt gccggaattc ggctttcgcg
     1741 ggctcaagcc cggcgaccgt tggtgcctct gcgcaccccg ttggcaagaa gctctcgagg
     1801 ccggccaagc gccgcgcgtg gtcttgcgcg ccacccacga aggcgcgctc gactactgct
     1861 cgctcgccga cctcaagcgc ttcgcggtgg acctcgcctg agtccggctg ccgtggcggt
     1921 cgcgtacgtc cttctcggct tagtggtcgt catcggtctc gtcgccgtca tatggcgcgt
     1981 cggctcccgc cgtcattcgg ttccctgccc ggtatggctg cgctggctgg tggagctcga
     2041 cagtcccttc acgaaaacca atcgggcggc ggtcatcgtc gagcgtctgg atctgcagcc
     2101 gggcatggcg gtgttggatg ccggctgtgg tcctggccgc cttgccatcc ccatcgccag
     2161 gcacgtcggg ccgcgaggcg aagccgtggc catggatatc caagccggga tgcttgagcg
     2221 cgcgcgcgaa aaagcagcgg ccgcaaacct cgccaatatt cgatttctcg agggcgggct
     2281 gggagagggc aagctcggcc gcaaccggtt cgaccgctcg gtgctggtga cggtcctggg
     2341 cgaggtcccg gatcgcgagg cggcgctgcg ggagattttc gatgctttga agcccggcgg
     2401 catcctgtcc gtgaccgaga tcattttcga tccccatttc cagtcgcgcc cgacggtcgc
     2461 gcgactggcg cgctcggtcg ggtttcgcga gcaagcgttc catgggaatc gcgttgcatt
     2521 cacgttgaac ttggaaaagc catacgatgc ggcgccgcaa cgagcgtgaa agattgcgac
     2581 gccctgcgct cattctcgcg ccagcgggaa tgcagaaaga gctgtccccg ctttcgcggg
     2641 gaccagcgga tcttgcgaat acaggtctcg cgggagctta gaacgcgcga cgagcgccgt
     2701 tagcggaacc ccaggcaaag aagttca
//
LOCUS       DATLJT010001396         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4026010,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001396 DATLJT010000000
VERSION     DATLJT010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4026010"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1811
                     /locus_tag="VM910_32005"
     CDS             129..1811
                     /locus_tag="VM910_32005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI67165.1"
                     /translation="MLLPAGVTVQDENGNLLLVNDIAAAQLGVSQNEAVEPSPQLIQR
                     RSQGLELLRAGRAAVTEDSTGDGPLKQVLLTSHRPVRIDGRNLLLSCSADISEQKAFE
                     DQLFRSAYYDELTDLPSRRVIEHRVNGLLQREAANEKFALAFLDIDNFKHINDYYGHA
                     VGDALLTGLAKRLGRELRETDMLSRISGDEFLLLLCPVQGSHEVIEFIEQTLQRLRAP
                     FFIDGSEIFASTSIGISLYPDHGTNYEILRQNADLAMYRVKNDGKGAAAFFDAGMEQE
                     ALVRTRIEQSLRLAILEKRFCCAFQAKVDIRTQEIKGIEALVRLRDDESVIQAPGSFI
                     NLAVELGLIDDLTHLVLAEIVKSIDLINESFGNNTTISINVAAKQAGNLQFMRSFAGA
                     LEATGYPNRFMIEVTEDAFVTRTHFQDEIVPILRKIGVGISIDDFGIGYSSLSALADI
                     TADEIKIDRSFITDIHKRPRSQGILRAIESLSEALGMTVVAEGIESFEELAYLQAATK
                     IRYAQGFYFSRPVFLEELKPALPLASEARVSLASRPVQESRPAHSRGRAYGR"
BASE COUNT          438 a          678 c          617 g          401 t
ORIGIN      
        1 taagagggaa ctgcccacaa tccgcggcca tgcggcgtca aatagtcaac gaattgcgga
       61 tttccgccac ggagggcgac ggcccctgcg cggtcgaggc agaactgaac gctctgaagg
      121 acgcgctcat gctcctgcct gccggtgtca cggtgcagga cgaaaatggc aacctgctgc
      181 tggtgaacga tatcgcggcg gcccaactcg gggtgtccca aaacgaggct gtcgagcctt
      241 cgccgcaact gatccagcgc cgcagccaag gcctggagct gctccgagcc gggcgcgccg
      301 ccgtcaccga ggactcaacc ggcgacggcc cgctcaagca ggtcttgctc acgagccacc
      361 gccccgttcg catcgacggc cgcaatctcc tgttgtcatg ctcggccgat atcagcgagc
      421 agaaggcgtt cgaggaccag ctcttccgct cggcctatta tgacgaactg accgaccttc
      481 cgtcgcggcg ggtgatcgag catcgcgtca acggcctgtt gcagcgtgaa gccgcgaacg
      541 agaaattcgc gctcgccttt ctcgacatcg acaatttcaa acatatcaac gattattacg
      601 gccacgcagt tggcgatgcg ctgctgaccg ggcttgccaa gcggctgggg cgcgagttgc
      661 gcgaaaccga catgctgtcg cggatttccg gggacgaatt tctgctgctg ctctgcccgg
      721 ttcaaggcag tcacgaagtg atcgaattca tcgagcagac cttgcagcgg ctcagagcgc
      781 cgtttttcat cgacggctcg gaaatcttcg cctcgacctc gatcgggatc agcctgtatc
      841 cggatcacgg cacaaattac gagatactgc gccagaacgc cgaccttgcg atgtaccgcg
      901 tcaagaacga cggcaagggc gcggccgcct tcttcgatgc cggcatggag caggaagcgc
      961 tcgtgcgcac caggatcgag cagtcgttgc ggcttgctat cctggaaaag cggttctgct
     1021 gcgccttcca ggccaaggtc gacatcagga cgcaggagat caagggcatc gaggccctgg
     1081 tacgcctgcg cgacgacgag agcgtgatcc aggcacccgg ctccttcatc aatcttgcgg
     1141 tcgagctcgg cctgatcgac gacctcacgc atctggtgct ggccgagatc gtcaaatcga
     1201 tcgacctgat caacgagtcg ttcggaaaca acaccacgat cagcatcaat gtcgcagcca
     1261 agcaggccgg caacctccaa ttcatgcgct ccttcgccgg ggcgctggag gcgaccggct
     1321 atcccaaccg cttcatgatc gaggtcaccg aggacgcttt cgtcaccaga acgcatttcc
     1381 aggatgaaat agtgcctatc ctcaggaaga tcggagtggg aatctcgatc gacgatttcg
     1441 gcatcggcta ttcgtccctg tcggcgctgg ccgacatcac cgccgacgag atcaagatcg
     1501 accgctcctt catcaccgac attcataagc ggccgcgcag ccagggcatc ctgcgcgcaa
     1561 tcgaatcctt gagcgaagca ctcggcatga cggtggtcgc cgagggcatc gaatccttcg
     1621 aggagctcgc ttacttgcag gctgcaacca aaatccgcta cgcgcagggc ttctactttt
     1681 cccgacccgt gttcctggaa gagctgaagc cggccctccc gctcgccagc gaagcgcgtg
     1741 tcagccttgc aagccggccg gtgcaggaaa gccggcccgc ccattcgcgc ggacgcgctt
     1801 acggccgtta gagtatgctc gggaagagtc gtcgccctcc gggcgttaag tctgagttaa
     1861 ctcaaactct ccgcacaaaa taccgattaa atcgaaagta actggatttc ctacggctta
     1921 tgccatttcg gcgtcgtatt catccccttc gaggggaaat gcgcgttgat ttccagcatt
     1981 cagggcaaat cgtcaaaagc cgccaccgcc acgtggcggc ggctcacatt cgggcagccc
     2041 cgcgacccca tcttctggct gatcgcgagc ggctgcatcc ttgttgcggc gatctgcgtc
     2101 ggcaccatga tcatgatcgg tgaattccgc gagc
//
LOCUS       DATLJT010001397         8645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3107007,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001397 DATLJT010000000
VERSION     DATLJT010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107007"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="VM910_32010"
     CDS             complement(<1..1283)
                     /locus_tag="VM910_32010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVI67166.1"
                     /translation="MTFKRPYGAGLAALAIGLFAATAAYAEPTVNKGDNAWMLTSTVL
                     VLLMTIPGLALFYGGLVRSKNMLSVLMQVFYTVCIVVVIWALYGYSLAFTGGSDFIGG
                     FSKAFLMGVTPDSKAATFSVDANISELVYVCFQMTFAAITPCLIVGSFAERTKFSAVA
                     LFIPLWVTVIYFPIAHMVWYWAGPDAISDAVKALAAATDDAAKAAAQAKLDEVNADAG
                     WIFKKGAIDFAGGTVVHINSGIAGLVGCLLVGKRTGYGKDLMAPHSLTMTMIGASLLW
                     VGWFGFNAGSNLEATGGAALAMTNSFVATAAAALAWMFAEWIIKGHPSVLGALSGAVA
                     GLVAVTPAAGYSGPMGAIVLGLVVGVVCLFFCTVVKNALGYDDALDVFGVHCVGGIVG
                     ALGTGILVNPALGGTGIMDYTTGKIADYDFVAQMTS"
     gene            complement(1321..1659)
                     /locus_tag="VM910_32015"
     CDS             complement(1321..1659)
                     /locus_tag="VM910_32015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVI67167.1"
                     /translation="MKIVMAIIKPFKLEEVRDALTAIGVHGLTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKIKIEVAIASDQVDKTIDAITSAAKTGQIGDGKIFVMNLDHAVRI
                     RTGEADAAAL"
     gene            complement(2034..2372)
                     /locus_tag="VM910_32020"
     CDS             complement(2034..2372)
                     /locus_tag="VM910_32020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVI67168.1"
                     /translation="MKLVVAIIKPFKLDEVRQALTAIGVHGMTVTEVKGYGRQKGHTE
                     IYRGAEYVVNFLPKLRIEIAVPSDLAEKAIDVITSNARTGQIGDGKIFVTPLDHALRI
                     RTGETDSDAL"
     gene            2583..3470
                     /locus_tag="VM910_32025"
     CDS             2583..3470
                     /locus_tag="VM910_32025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HVI67169.1"
                     /translation="MPERSWFFAAEGKQQGPYPDAQFRGFIARRMVQAETLVWTEGMS
                     GWQKAGDVPGLIASAGSPPTLPGSTGAVMSGGDHGGGSLSIDLEIWEFVWRCLVCFLG
                     LITVIAAPWAIVWYAKWLVSRVQVPGRPNLSFTGSALTIAAWYFGFIVLAIIVAMIES
                     QTLGDLLSIVQIALYWLFLRWFIANLASNGQPLGLSFSGSLWAYLGWSLLMVFSVITI
                     IGWAWVYTAQMRWICRNIQGTRRQVVFHGAGLEYLWRVIVALLLGSLIIPIPWVARWL
                     LNWQLSQTALVERHAYANA"
     gene            complement(3477..4337)
                     /gene="tesB"
                     /locus_tag="VM910_32030"
     CDS             complement(3477..4337)
                     /gene="tesB"
                     /locus_tag="VM910_32030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HVI67170.1"
                     /translation="MSKSLIDLISILDLEPIEVNLFRGNSPKTSWQRVFGGQVIGQAM
                     VAACRTVEGRLPHSIHCYFILPGDPQIPIIYEVERLRDGRSYSTRRVTAIQHGNAIFS
                     IMVSFHAEEKASFDHQDKMPDVPPPEKLTAEEVAKQPMFREMPDFIRRYYESDRPIEL
                     RPVELGRYFGQKIDDGRIHIWIRTAAKLPDDPALHMCALAYASDFSLLDAVTARYGRT
                     LFDKRMMPASLDHAMWFHRPFRADEWLLYAQDSPSAQAGRGLTRGLIFKPDGTLIASV
                     AQEGSVRERR"
     gene            4446..5666
                     /locus_tag="VM910_32035"
     CDS             4446..5666
                     /locus_tag="VM910_32035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis hydroxylase"
                     /protein_id="HVI67171.1"
                     /translation="MSARRSIVIGGGAIAGLALALALRQGLGADVAVIVADPALASRP
                     SRDPRATAIVAACRRLFETLGVWGEVADNAQPILDMVITDSRLEDATRPVFLTFAGNV
                     EPGEPFAHMVENRYLIDALAKHAEANGVELKATAVASYESHGDAVDVRLADGGAIEAS
                     LLIAADGARSKLRERAGIATNGWDYDQSGIVVTVGHEREHHGRAEEHFLPAGPFAILP
                     LTGKRSSLVWTEQRKEAARIVGLSDDEFHGELERRFGLHLGEVKALDKPRAFPLSYFV
                     ARSFIAERMALVGDAAHVIHPIAGQGLNLGLKDVAALAEVVVDAARLGIDFGQSDVLE
                     RYQRWRRFDTMAMGLATNSLNFLFSNKSTLLRTVRDIGLGLVDRTPPLKEMFIRQAAG
                     LSGEIPRLLKGEAL"
     gene            complement(5672..6607)
                     /locus_tag="VM910_32040"
     CDS             complement(5672..6607)
                     /locus_tag="VM910_32040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI67172.1"
                     /translation="MRAIATAVTYLVLVLAFAATARAGESKIRVGPTDAYLTVPPEIE
                     RPPVALLIAGSGSIDHDGNGPKVKPATLKKLAEQLAARGIATLRYDKRGAGGWKTEFG
                     DPKDFRFKDYVDDAAAMVGYLRDSKKFSKIILAGHSEGGLVAILTAHRVPVDGLVLLA
                     TAARREGDVVKAQLEKKLPPETFAPMAKAIDAIMAGQIVDPLPPGLAIPPALQPSIAS
                     VFTEDPIDPLSKLDTSTFIVAGGRDSQLARLDFLALGTAAPAAKTLWLPEMNHVLVDV
                     TDETDDLAAYDQPERPLDPALIDAVAAFILSGAAR"
     gene            complement(6609..6806)
                     /locus_tag="VM910_32045"
     CDS             complement(6609..6806)
                     /locus_tag="VM910_32045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HVI67173.1"
                     /translation="MTSSPERLENTVDPKLLEILVCPITKGPLEYDAARQELISRSAK
                     LAYPIRDGIPIMLPEEARKIA"
     gene            complement(6925..7461)
                     /locus_tag="VM910_32050"
     CDS             complement(6925..7461)
                     /locus_tag="VM910_32050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI67174.1"
                     /translation="MSDQLSARDWLDQGLKTLAANGFSALKAEPLAKAMGVSRGSFYW
                     HFADVGAFRTAILKHWREVAAERVIADLETAAAQENPLLLLLRRTFSSRLALEKAVRS
                     WASFDAEARAAVQAIDRRRVDYVAQLLRSNGLATGIAEARAQILYWTFVGYALSDKPL
                     AKAKQEALLDELVRIAMR"
     gene            7544..7894
                     /locus_tag="VM910_32055"
     CDS             7544..7894
                     /locus_tag="VM910_32055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67175.1"
                     /translation="MRDILLQSSGALAILVALIHGVLTETKVFPRVTIEPQRLRTLFR
                     LVWQIPTVAWIACGVLLIAAPSMASDVARHWMVVMFAVVFAFSAFGNAVGTRGKHFGW
                     MMLSAVTVLAVAGY"
     gene            complement(7910..8584)
                     /locus_tag="VM910_32060"
     CDS             complement(7910..8584)
                     /locus_tag="VM910_32060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HVI67176.1"
                     /translation="MPINIEYRGPADLPEVIPVFPLPGALLLPRGQMPLNIFEPRYLS
                     MVDDALRDGHRLIGMIQPDITHSQSEEKPVLFHVGCVGRITQLAESGDGRYILELTGV
                     SRFRVVEEMTVLTAYRQCRVDYFPFIDDFTARKGEDAVNRAALLRVLTDFLKANNLKV
                     DWADVESAPNEALVNALAMMSPYGPAEKQAMLEAPDLKTRAEILIAVTEMDLAKKRTS
                     GDPPLQ"
BASE COUNT         1523 a         2659 c         2813 g         1650 t
ORIGIN      
        1 gaggtcatct gcgcgacgaa gtcgtagtcg gcgatcttgc cggtggtgta gtccatgatg
       61 ccggtgccgc cgagcgccgg gttcaccagg atgccggtgc cgagcgcgcc gacgatgccg
      121 ccgacgcagt gaacgccgaa cacgtcgagg gcgtcgtcat agccaagggc attcttcacc
      181 acggtgcaga agaacaggca gaccacgccg accacgagac cgagcacgat cgcgcccatc
      241 ggaccggaat agccggccgc cggcgtcacc gcgacgaggc ccgcgactgc acccgacaat
      301 gcgccgagca cggacggatg gcccttgatg atccactccg cgaacatcca ggccagcgcc
      361 gcggctgcag tcgcgacgaa ggagttggtc atggcaagcg ctgcgccgcc ggtggcctcc
      421 aggtttgaac cggcgttgaa gccgaaccag ccgacccaga gcagcgaggc gccgatcatg
      481 gtcatcgtta gcgagtgcgg agccatcagg tccttgccgt agccggtgcg cttgccgacc
      541 agcagacagc caaccagacc ggcaatgccg gagttgatgt gcaccacggt tccgccggcg
      601 aagtcgatgg cgcccttctt gaagatccag ccggcatcgg cattgacttc gtcgagcttg
      661 gcctgggccg ccgccttggc cgcatcgtca gtcgccgctg caagcgcctt caccgcatcc
      721 gagatcgcgt ccgggccggc ccagtaccag accatatgcg cgatcgggaa atagatcacg
      781 gtcacccaaa gcgggatgaa cagggccacc gcagagaact tcgtgcgctc ggcgaacgag
      841 ccgacgatca ggcagggcgt gatcgccgcg aaggtcatct ggaagcagac atagacgagc
      901 tcggagatgt tggcgtcgac gctgaaggtg gcagccttgg agtccggcgt cacgcccatc
      961 aggaacgctt tcgagaagcc gccgatgaaa tcggagccgc cggtgaaggc aaggctgtag
     1021 ccgtagagag cccagatcac gacgacgatg cagaccgtgt aaaacacctg catcaagacc
     1081 gacagcatgt tcttggaccg aaccaggccg ccatagaaca gcgcgaggcc gggtatggtc
     1141 atcaacagca cgagcaccgt ggatgtcagc atccacgcat tgtcgccctt gttgacggta
     1201 ggctccgcat aggcagcggt cgcggcgaac agaccgattg cgagggccgc caatcccgcg
     1261 ccgtagggac gcttgaacgt cattttatat tactcctgga tgataagtgt gagcgcgaga
     1321 tcagagggcc gcagcgtcgg cttctccggt gcggatgcgc accgcgtggt cgaggttcat
     1381 gacaaagatc ttgccgtcgc cgatctgtcc ggtcttggcg gcggacgtga tggcgtcgat
     1441 cgtcttgtcg acttgatcgg aggcgatggc cacctcgatc ttgattttcg gcaggaaact
     1501 cacggcatat tcggcgccgc gatagatttc cgtgtggcct ttctggcggc catatccttt
     1561 gacttccgtc accgtgagcc cgtgaacgcc aatggcggtc agggcgtctc ggacttcttc
     1621 cagcttgaat ggcttgatga tcgccataac aattttcatg ggtcctgtcc ccgcttgggc
     1681 ccggccagag aaactgaccg ggcgtgttct cgactgaggt ttgccacgcg gagagagaag
     1741 atcactacgc ggccacagcc ggccccttag aatcaaatgc cgtgccagat cggccgcagg
     1801 ccctaacgga ctacaattgc ggatgttttt gcggttgacg aaacgggctg gaacctgcgc
     1861 tattcggtcg cgcctaaaat ctggacaggc ctgattaaat cctgtgctag tcgcgatatc
     1921 gacataatcc tgctcacgac gttggcagga ttttttccaa attcagttga ttgctgaggc
     1981 gcttggccgc gctggagagt gccgacgtcc gccggatgcg aaggcgagag cggtcaaagc
     2041 gcgtcgctgt cggtttcccc ggtccggatg cgcagcgcgt gatcgagcgg cgtgacgaag
     2101 atcttgccgt cgccgatctg gccggtgcgc gcattggagg tgatgacgtc gatcgccttc
     2161 tccgcgagat cggacggcac ggcgatctcg atgcgcaatt tgggcaggaa attcacgaca
     2221 tattcggcgc cgcggtagat ctcggtgtga cccttctggc ggccatagcc cttgacctcc
     2281 gtcaccgtca tcccatgcac gccgatcgcg gtgagggctt ggcgcacctc gtcaagtttg
     2341 aacggtttga tgatcgcgac gacgagcttc atggtcgggc ggttccttac ctggttgccg
     2401 attgagtgga ttctcggtcg gaggcagcca actctgacat ctttctgggc aaatggcaca
     2461 aagaatttgc agcacggctg gggattcttg gccggccgcc tcccaccggg cctgtacagg
     2521 ccgacgaggg gtccaccaca atggtttcgg cgagtcgcga aggcgagtca tggggaaacg
     2581 agatgccgga acgatcctgg ttctttgcgg ccgaaggcaa gcagcaaggc ccctacccgg
     2641 acgcgcagtt tcgcggcttc atcgcgcgcc gcatggtgca ggcggagacg ctggtctgga
     2701 ccgagggcat gtccggctgg caaaaagccg gtgacgttcc gggcctgatc gccagcgccg
     2761 gttcgccgcc cacgctgccg ggatcaaccg gcgctgtgat gagcggtggc gatcacggcg
     2821 gcggttcgct gtcgatcgat ctcgaaatct gggagttcgt ctggcggtgc cttgtgtgct
     2881 tcctcggcct catcaccgtg atcgcagcgc cctgggccat cgtctggtac gcgaaatggc
     2941 tggtgtcccg cgtccaggtt ccggggcggc cgaatctctc gttcaccgga agcgcgctca
     3001 ccatcgcagc gtggtatttc ggatttatcg tgcttgcgat catcgtggcc atgatcgaaa
     3061 gccagacgct cggcgatctc ttgagcatcg tgcaaattgc tctctactgg ctcttcctcc
     3121 gatggttcat agcgaacctc gcctcgaacg ggcagccgct gggcttaagc ttctcagggt
     3181 cgttgtgggc ctatctcgga tggtcgctgt tgatggtttt ttccgtcatc accatcatcg
     3241 gctgggcatg ggtttacacc gcgcagatgc gctggatctg ccgcaacatc caaggcacgc
     3301 gccggcaggt cgtcttccat ggcgccggcc tggaatatct ctggcgcgtc atcgtcgccc
     3361 tgctcttggg ctcgctgatc attccgatcc cctgggtggc gcgctggctg ctgaactggc
     3421 aactgtcgca aaccgcgctg gtggagcggc acgcttatgc aaacgcgtga acggatttac
     3481 cgcctttcgc gcacagagcc ttcctgcgcg acggaggcga tcagcgtgcc gtcaggcttg
     3541 aagatcaggc ctcgcgtcag tcctcggccg gcctgcgcgc tcggggaatc ctgcgcatag
     3601 agcagccatt cgtcggcgcg gaacgggcga tgaaaccaca tcgcatggtc aaggctcgcc
     3661 ggcatcatcc gcttgtcgaa caatgtgcgg ccgtagcgcg ccgtcacggc atcgagcagc
     3721 gaaaaatccg acgcataggc gagtgcgcac atatgcaagg ccgggtcatc gggcagtttt
     3781 gcggcggtgc ggatccagat atggatacgg ccgtcgtcga ttttctggcc gaaataacgg
     3841 ccgagctcca cgggtcgcaa ttcgatcggg cggtcggatt cataatagcg gcggatgaaa
     3901 tccggcatct cgcgaaacat cggctgcttg gccacctctt cggccgtaag cttttccggc
     3961 ggcggcacgt cgggcatctt gtcctgatga tcgaacgaag ctttttcctc ggcgtgaaac
     4021 gacaccatga tcgaaaagat cgcgttgccg tgctggatgg cggtgacccg ccgcgtgctg
     4081 tagctcctgc cgtcgcgcag acgctcgacc tcgtagatga tcgggatctg cggatctccg
     4141 ggcagaatga aatagcaatg aatggaatgc ggcaggcgcc cctcgacggt gcggcaggcc
     4201 gcgaccatcg cctgcccgat cacctggccg ccgaacaccc gctgccagct cgtcttcggg
     4261 ctgttgccgc ggaacaaatt cacctcgatc ggctcgagat cgaggatcga gatcaggtcg
     4321 atcaggcttt tggacatagc gcacctttcg aaccggccaa ggacggaact accgtcgtcg
     4381 gtcttgtttg gtgaacctgc ttcccccagc tataatatac gaagtgtacg agtaagcagg
     4441 ctctcatgtc agcacggcga agcatagtca ttggcggcgg cgcaatcgcc gggctggcgt
     4501 tggcactggc cctgcgtcag ggcctgggcg ccgatgttgc cgtcatcgtg gccgacccgg
     4561 cgcttgcgtc gcggccgagc cgcgatccgc gcgcaaccgc catcgtcgcc gcgtgccggc
     4621 ggctgttcga gacgttgggc gtgtggggcg aggttgcgga taatgcgcag ccgatcctcg
     4681 acatggtcat caccgactca aggcttgagg acgcaacccg tccggtgttt ttgaccttcg
     4741 ccggcaatgt cgagccgggc gagccgttcg cgcacatggt cgagaaccgc tatctgatcg
     4801 atgcgctcgc caagcatgcc gaggccaacg gtgtcgaact caaggctacc gcggtcgcat
     4861 cctacgagtc gcatggcgat gccgtcgatg tgcgccttgc cgatggcggt gcgatcgaag
     4921 cgagtctgct gatcgcagcc gacggcgccc gctcgaagct cagggagcgg gcgggcattg
     4981 cgaccaacgg ctgggattac gatcagtccg gcattgtcgt cacggtcggc catgagcgcg
     5041 agcatcacgg ccgcgcggag gagcatttcc ttcccgccgg tccctttgcg atcctgccgc
     5101 tcacgggaaa gcgttcctcg ctggtatgga ccgagcagcg caaggaggcg gcgcgcatcg
     5161 tcggcctgag cgacgacgaa tttcatggcg agctcgagcg gcgcttcggc ctgcatctcg
     5221 gcgaggtgaa agcgctcgac aagccgcgcg ccttcccgct gtcctatttt gtcgcacgct
     5281 ccttcatcgc cgagcgcatg gcactggtcg gcgatgccgc gcatgtgatt cacccgatcg
     5341 cgggtcaggg cctcaacctg ggcctgaagg atgtcgcggc gctcgccgaa gtcgtggtcg
     5401 atgcggcgcg gctcggcatc gacttcgggc agtcggatgt gctcgagcgc taccagcgct
     5461 ggcggcggtt cgacaccatg gcgatggggc ttgccaccaa ttcgctgaac ttcctgttct
     5521 ccaacaagtc gacgctgttg cgcacggtgc gcgacattgg gctcggcctc gtcgatcgca
     5581 ctccgccgct gaaggaaatg ttcatccgcc aggccgccgg actttccggc gaaatcccgc
     5641 gcctgttgaa gggcgaagcg ctgtaaacat gctaacgcgc ggcgcctgag aggatgaacg
     5701 ccgccaccgc atcgatcagc gcggggtcga gcggccgctc cggctggtcg taggcagcga
     5761 gatcgtccgt ctcgtcggtg acatcgacca gaacgtgatt catctccggc agccacagcg
     5821 ttttcgcggc gggcgccgca gtgccgagcg ccagaaaatc gagccgcgcc agttggctat
     5881 cgcggccgcc cgcgacgatg aatgtcgacg tgtcgagttt gctcaaggga tcgatcggat
     5941 cttcggtgaa gaccgaggcg atggagggct gcagcgccgg cgggatcgcg agacccggcg
     6001 gcaagggatc gacgatctgg ccggccatga tggcgtcgat cgctttcgcc atcggcgcga
     6061 acgtttcggg cggaagcttc ttctcgagtt gggccttcac cacgtcgcct tcccgccgcg
     6121 cagcggtcgc aagcagcacc aggccgtcga cgggaacgcg atgggcggtg aggatcgcga
     6181 cgagtccgcc ctcgctgtgg ccggccagga taatcttcga aaacttcttg ctgtcgcgaa
     6241 ggtagccgac catcgccgcc gcatcgtcga cgtaatcctt gaagcggaag tctttggggt
     6301 cgccgaactc cgttttccat ccgcccgcgc cgcgcttgtc atagcggagc gtcgcgatgc
     6361 cgcgtgcggc cagttgttcg gcgagctttt tcagcgtggc cggtttgacc ttggggccgt
     6421 tgccgtcgtg gtcgatggag ccggagcccg cgatgagaag cgcaacgggc ggccgctcaa
     6481 tttcgggcgg aacggtgaga taggcatccg tcgggccgac cctgattttg ctttccccgg
     6541 cgcgcgcggt cgcggcgaaa gcaagaacaa gaaccaggta tgtaaccgca gtcgcgatcg
     6601 cccgcatctt atgcgatctt gcgcgcctct tccggcagca tgatcgggat gccgtcgcgg
     6661 atcggatagg cgagcttggc cgagcgcgag atcagttcct gcctcgccgc atcgtattcc
     6721 agcggaccct tggtgatcgg gcagaccagg atttcaagga gctttggatc gacggtgttt
     6781 tcgaggcgtt cgggcgaact ggtcatacgc gcagtctccg gcttcacgca cgccccttag
     6841 catattcgcc gccggtggag atacctcttt tggtcattgt gctttctcca cgaggatcgg
     6901 tgcccgcctc gcttgaaagc gcctctagcg cattgcgata cgcacgagct cgtcgagcag
     6961 cgcctcctgc ttcgccttcg ccagcggctt gtccgagagc gcgtagccca cgaaagtcca
     7021 atagaggatt tgggcacgcg cctcggcgat gccggttgca agtccgttcg atcgcagaag
     7081 ctgcgcgaca tagtcgaccc gccgccggtc gatcgcctgc acggcggcgc gcgcctcggc
     7141 atcgaacgac gcccagctcc gcactgcctt ttccagcgcc aggcggctgg aaaaggtccg
     7201 gcgcagcagc agcagcaggg gattttcctg ggccgcggcg gtctcgaggt cggcaatgac
     7261 ccgctcggct gcgacttccc gccaatgttt caggatcgcg gtgcggaacg cgccgacatc
     7321 ggcaaagtgc cagtaaaagc tgccgcgcga cacgcccatc gccttggcca gcggctcggc
     7381 cttcagggcg gaaaaaccgt tcgcggccag cgttttgagg ccctggtcga gccagtcacg
     7441 ggctgagagt tgatcggaca tgatgtcgcg caccatacag gagtgtattg acgggagcca
     7501 gtatcggcgt atgctaccat acagcactgt atggaggttc gttatgcgcg atatcctgct
     7561 gcaaagttcg ggggccctcg cgatcctggt cgcactcatc cacggcgtgc tgacggaaac
     7621 caaggtgttc ccgcgggtga ccatcgaacc gcagcgcctg cggacgctgt ttcgcctggt
     7681 ctggcagatt ccgacggtgg cctggatcgc ctgcggcgtg ctgttgatcg cggcgccctc
     7741 gatggcgtcg gatgtggcgc gccactggat ggtcgtgatg tttgcggtcg tgtttgcttt
     7801 cagcgcgttc ggcaatgcgg tgggcacgcg aggcaagcac ttcggatgga tgatgctgag
     7861 cgcggtgacg gtgctcgcgg tcgcgggcta ctagagcgtc tgccgggcgt cactgcagcg
     7921 gcgggtcgcc gctggtgcgt ttcttggcga ggtccatctc ggtgacggcg atcaaaatct
     7981 cagcgcgcgt cttcaggtcc ggcgcctcca gcatcgcctg tttctccgcg ggaccgtagg
     8041 gcgacatcat cgccagcgca ttgaccagcg cctcgttggg ggcgctctcg acatcggccc
     8101 aatcgacctt gagattgttg gccttcagga aatccgtcag cacccgcagc agcgccgcgc
     8161 ggttgaccgc gtcctcgccc ttgcgcgcgg tgaaatcgtc gatgaaggga aagtagtcga
     8221 cccggcactg acggtacgcg gtcagcacgg tcatctcctc gaccaccctg aagcgggaga
     8281 ctcctgtcag ctccaggatg tagcggccgt cgccggattc ggcgagctgg gtgatgcgcc
     8341 cgacgcagcc gacatggaac agcaccggct tttcctcgct ctgcgagtgc gtgatatcgg
     8401 gctggatcat cccgatcagg cggtggccgt cgcgcagcgc gtcgtcgacc atcgagagat
     8461 aacgcggctc gaaaatgttg agcggcatct gcccgcgcgg caacaacagc gcgccgggca
     8521 gcggaaacac cgggatcacc tccggaaggt cggccggtcc gcgatattcg atattgatcg
     8581 gcatcagcgc gcggcctcga gtttcagcgt ggtatcccgc gaaccgctcg agcgcgtcgc
     8641 ggaag
//
LOCUS       DATLJT010001398         3191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2876185,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001398 DATLJT010000000
VERSION     DATLJT010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2876185"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1186)
                     /locus_tag="VM910_32065"
     CDS             complement(20..1186)
                     /locus_tag="VM910_32065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67177.1"
                     /translation="MLNTKVKKTNFFDMFSLLPLGGAPTACDISTTFSDSEAVAVYDK
                     CASYTCQTARFGKAFGPMMRITFVLALLLSTFGPSRAIAQNTPTWSPIRPVRIVIPFQ
                     AGGPIDSIGRIVGQQLSEQWGQSFIVENRTGGGGNIGMELVAKAPADGYTLGTASGGT
                     HGANATLYGSKLPFDPVKDFTPITMLAEMKNILVVHRSLSITNLQELVAYAKKAPGQL
                     TFGSSGTGTIQHLTGEMFKAAAKIGIVHVSYRGQAQALPDLLGGRISMMFLGAGDAAE
                     HVRSGLIVPIGIASAERSALLPNVLPLAEQGLPGFNAVTWFGLVGPSGIPANIANAYY
                     ENISASLGQPEIGVRLLQIGLDPLTMRSKEFGRFIAGEVEKWGSVIRSVGIRID"
     gene            1146..1982
                     /locus_tag="VM910_32070"
     CDS             1146..1982
                     /locus_tag="VM910_32070"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HVI67178.1"
                     /translation="MSKKFVFLTFVLSTLGMPSIGWAQAPVQLKYTRVVDLTLPIESN
                     MEGIPGLKSYAENPSRVTVIAAITEAQRELLRAEGMTLSNNLVVNGRSMISVLSILVH
                     NGTHIDAPRHMIDKGEPVDQVPLERVAKEAVLINLPNKGPNSSVSVKDILDTGVEFGP
                     NIIPVINTGWTEKTWGKPEFWQQMPYLEPGVGELMAKRNVPAVAMDVFPEKAIWRGIK
                     LDPGEVWVANHLALLEKGIPMIQFVTNLSQIGKNRFVMIAAPLKIKGGDASPARALAL
                     VE"
     gene            2178..>3191
                     /locus_tag="VM910_32075"
     CDS             2178..>3191
                     /locus_tag="VM910_32075"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI67179.1"
                     /translation="MLAPEDKLKLVPKPYLEATARFANGADRPRDLLERCLADLAALE
                     PKIGAFVHLNLERARAAADQSTERWREGRPRSPIDGMPLGIKDIIETADMPTENGSPL
                     FAGWRSERDAASVAALREAGAVIVGKTVTTEFAATEPRGTRNPHDLRRTPGGSSSGSA
                     AAVAAGMLSAALGTQVIGSTIRPASYCGCIGFKASVGALNRGGSYDGLSQSATGVLAA
                     TLEDVWQVAYEIAARAGGDPGYPGLFGQPRAPALRKPRRLAFLQTAGWATASAGAKAA
                     IAEALERIKSAGIEIITSQTHNRVAAVAAAIEDARPLSMRINAWEWRWPLNTYRERDR
                     GKLS"
BASE COUNT          698 a          913 c          964 g          616 t
ORIGIN      
        1 ctgtttttgg agttggacat cagtcgatgc ggatgccgac cgacctgatc acgctgcccc
       61 atttctcaac ctcgccggca atgaagcggc cgaactcttt cgagcgcatg gtgagagggt
      121 caagtcctat ttgtaacagg cgtactccaa tctcaggctg accgaggctc gcggagatat
      181 tttcgtaata tgcgttggca atgttggccg gaatacccga cggacctacg aggccaaacc
      241 atgttacggc attgaaaccc ggtaatcctt gctcggcgag cggcaacacg tttggcaaga
      301 gggcggaacg ctccgcggaa gcgattccaa tcggcacaat caaacctgac cgaacatgtt
      361 cagcggcatc tcccgcgccg aggaacatca tcgagatacg gcccccaagt agatcgggca
      421 gtgcttgcgc ctggcctcga taggagacgt gaacaatgcc gatcttcgcc gcggctttga
      481 acatctcgcc cgtgagatgc tggatcgtcc ctgttcctga agagccgaac gtcagttggc
      541 ctggtgcctt ttttgcataa gcaaccagct cctgtaggtt ggttatcgac agtgagcgat
      601 gcaccacgag gatgttcttc atctccgcga gcatcgtgat tggcgtgaaa tctttgacag
      661 gatcgaacgg caatttcgag ccatagagcg ttgcgttcgc accatgtgtt ccgcctgagg
      721 cagtgccaag ggtgtagcca tcggccggtg ccttcgcgac cagctccatg ccgatattgc
      781 caccgccacc ggtccgattt tccacgatga aagactgtcc ccactgctca ctgagctgct
      841 gaccgacaat tcgaccgatc gaatcgatcg gaccacccgc ttgaaacgga atcactatcc
      901 gaaccggtcg gatgggtgac catgtcggcg tgttttgagc aatcgcgcga gaaggtccga
      961 acgttgaaag aagcaaagcg agaacgaaag ttattctcat catggggcca aaagccttgc
     1021 cgaacctagc ggtttgacac gtatacgaag cgcacttatc gtacacagca accgcctcac
     1081 tgtcactgaa ggtcgtggaa atgtcgcatg ctgtgggtgc cccgcctaaa gggaggagcg
     1141 aaaacatgtc gaagaagttc gtctttttga cctttgtgtt gagcacgctc gggatgccat
     1201 cgatcgggtg ggcccaggca ccggttcagc tgaaatacac tcgggtggtc gatctgacgc
     1261 tgccaatcga aagcaatatg gaaggaatcc ctggactaaa atcctacgcg gagaaccctt
     1321 ccagagtgac cgtaatcgca gccatcaccg aggctcaaag ggagttattg cgggccgaag
     1381 ggatgacgct aagtaacaat ttggtggtca acggccggtc gatgatttcg gtcctctcca
     1441 tcctggtaca caacggcact cacatcgatg cgccccgaca catgatcgat aaaggagagc
     1501 cggtcgatca agttcccttg gagcgggttg cgaaagaggc agtgctgatt aacctgccga
     1561 ataagggacc aaattcgtcc gtgtccgtga aagacatcct agatactggg gtggagttcg
     1621 gtccgaatat tattccggta atcaatacag gttggacgga gaagacgtgg ggcaagccgg
     1681 agttctggca acagatgcct tatcttgaac ccggcgtggg tgagctaatg gcgaagagaa
     1741 acgtacccgc cgtggcgatg gatgtctttc cggaaaaggc aatctggagg ggaatcaaac
     1801 tcgatccggg tgaagtgtgg gttgcaaacc atctcgctct gctcgagaag ggcattccta
     1861 tgattcagtt cgtaaccaac ctttcccaga ttgggaagaa taggttcgtg atgattgcag
     1921 cgcccctcaa aataaagggc ggagacgcaa gtccagcacg tgcgctcgct ttggtagaat
     1981 gattctgacg ctatcgttca gagcgctgtg ccaaacgtac ttctcggcga gacggaacaa
     2041 gggacccgtc cttcgaagct cggtcaagcc gtgatcgaga tggttgtcca aatcggagcg
     2101 tggctgtctg ctctacaaga tccataggat tagggggagc aagacatagc ggcgctcaag
     2161 cagcggagcg catcaccatg ttggcgccgg aagataagct caagctcgtg ccaaagcctt
     2221 acctcgaagc cacggcacgg tttgccaatg gcgccgatag accccgcgat ctcctcgaac
     2281 gctgccttgc cgatctcgcc gcgctcgaac cgaagattgg cgcgttcgtt catctcaatc
     2341 ttgagcgcgc gcgtgcagcc gccgaccaat ccaccgagcg gtggcgcgag ggccggcctc
     2401 gctcgccgat cgacggcatg ccgctcggca tcaaggacat cattgagacc gccgacatgc
     2461 cgaccgagaa cggctcgccg ctgtttgcgg ggtggcgctc cgagcgcgac gctgcgagcg
     2521 tagcggcgct gcgcgaagcc ggcgcagtca ttgttgggaa gactgtgacc acggaattcg
     2581 cagccaccga gccgcgcggt acgcgcaacc cgcatgatct gcgacgcacg cccggcggat
     2641 cgagcagcgg ctcggcggcc gcggttgcgg cgggcatgct aagcgccgcg ctcggcactc
     2701 aggtgatcgg ctcgaccatt cgcccggcca gttactgcgg ctgcatcggc ttcaaggcga
     2761 gcgtcggcgc actcaatcgc ggcggtagtt atgatggatt gagccagagc gccacgggcg
     2821 tgcttgccgc cacgttggaa gacgtctggc aggtcgccta cgaaatcgcc gcgcgcgccg
     2881 gcggcgaccc tgggtacccc ggcctgtttg ggcagccgcg cgcgcccgca ctgcgcaagc
     2941 cgcgccgcct tgctttcctc caaacggcag gttgggccac ggcgtcggcg ggcgcgaaag
     3001 cggcgatcgc cgaggcgttg gagcggatca aatccgctgg gatcgagatt ataacgagcc
     3061 agacgcataa cagggtcgcc gccgtcgcag ctgcgatcga agacgcgcgt ccactctcca
     3121 tgcgcatcaa tgcgtgggaa tggcgctggc ccctcaacac ctatcgcgag cgggaccggg
     3181 gcaagctgag c
//
LOCUS       DATLJT010001399         6333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1269085,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001399 DATLJT010000000
VERSION     DATLJT010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269085"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            618..971
                     /locus_tag="VM910_32080"
     CDS             618..971
                     /locus_tag="VM910_32080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HVI67180.1"
                     /translation="MAEPKLEVPAELRDLAEKTIDQAEKAFGMFFDAAGKSISALPGA
                     GSDISKQALTFTEQNMKAAFEHARKLVHATDFQEAMRIQSEFLRSQFTNAGEHMRQIT
                     GQVMSAAKDASKSKF"
     gene            complement(1137..1457)
                     /locus_tag="VM910_32085"
     CDS             complement(1137..1457)
                     /locus_tag="VM910_32085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI67181.1"
                     /translation="MTASLKCLLILMMAAAAPLASRAMAADADHGADLAKRWCATCHV
                     VGSEQRQASADVPSFASIARRSDFTAEKIAFFLLDPHPKMPSFPLSRSEAADLAAYIG
                     SLKK"
     gene            complement(1563..2393)
                     /locus_tag="VM910_32090"
     CDS             complement(1563..2393)
                     /locus_tag="VM910_32090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67182.1"
                     /translation="MRRAFAILACLCALAAGEYLAISKLAIRHETLNLYDAARQRPVA
                     VDVAVRRDYEVKANLGLWKLPIAIISNGNTVKNTEYSFLANVLAARGYLVASIQQDLP
                     SDPPLMTKVGLPYVGRRGVYMRCEANILFVLGELQKLQPNAYYDHLTLVGHSNGGDVS
                     MYVAKQHPELVSKVITLDNLRVPFVLSNKLKILSFRSKDPNFKTDPGVLPTPTQAKTG
                     GIDIINTGALHTDLSDRGPDALKEKIQASLEKFLRDGGSSDLAPANTTQLMIANPGDY
                     "
     gene            complement(2708..2911)
                     /locus_tag="VM910_32095"
     CDS             complement(2708..2911)
                     /locus_tag="VM910_32095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67183.1"
                     /translation="MDTATDVSRTVGEVTASLKAAVERLSAVAAEVQSGRVIPAIRRA
                     TRRAPLTSLFGAFLLGVVVARRR"
     gene            3227..4204
                     /gene="rocF"
                     /locus_tag="VM910_32100"
     CDS             3227..4204
                     /gene="rocF"
                     /locus_tag="VM910_32100"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HVI67184.1"
                     /translation="MNNAFGGEPAQRIAVLGAPIDMGASQRGTLMGPAALRTAGLLTL
                     LESLGFDVADYGDLSIAAMADLADPPPKKANHYREIQRWTRALSRRGYELAQTGAIPI
                     FLGGDHSLSMGSVNAMARHWQGQGRELFVLWLDAHADYNTPTTTLTANMHGMAAAFLC
                     GEDGLETLLGEEPRASIGPDQLKLFGTRSIDKLEKDLLRARCVSVADMREIDEFGVGV
                     PIRRFIDKVRGRDGVLHVSFDVDFLDPAVAPGVGTTVPGGATYREAHLVMEMLHDSGL
                     VRSVDIVELNPFLDERGRTARTAVELIGSLFGQQITDRPTPSNAIEPGE"
     gene            complement(4226..5752)
                     /locus_tag="VM910_32105"
     CDS             complement(4226..5752)
                     /locus_tag="VM910_32105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI67185.1"
                     /translation="MASTEVLIVGAGPTGLVLALWLTKLGTRVRIVDKTAEPGTTSRA
                     LAVQARTLELYRQLDLADAVIARGHQVPAGNFWVKGKRRARISLNALGAGLTPYPFMQ
                     IFPQDEHEKLLISRLEALGVTVERETELLRFAEHDDGVTAHLRGPDGSEHDCSAIYLA
                     GCDGARSKARETIGAGFPGGTYPQVFYVADVDAAGPALNGELHVDLDEADFLAVFPLA
                     GQGRARLIGTMRGERAQRAETLTFADVSDRAINHMKVMVAKVNWFSTYHVHHRVTEHF
                     RRGRVFLAGDAAHIHSPAGGQGMNTGIGDAINLAWKLTAVLKGRGSDVLLDSYEAERI
                     GFAHRLVATTDRIFTFATGEGRLADFIRTRVAPVVIPAAFSFEAVRRFAFRTVSQTML
                     NYRGGPLSRGAAGQVHAGDRLPWVAVAGSDNFKPLTSMDWQVHVYGSAKGELSAWCAA
                     RNVPLHVFPWRDAHLSAGLARDALYLIRPDTYVALADESGHADALNRYFADLGIKPAP
                     "
     gene            5882..>6333
                     /locus_tag="VM910_32110"
     CDS             5882..>6333
                     /locus_tag="VM910_32110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67186.1"
                     /translation="MTITATAPAATSERTKINSIVFASCLGTIIEWYDFLIYATGAAL
                     VFNKAFFPTFDPLAGTLAALGSYAVGFLARPLGGALFGHFGDRLGRKSMLVLTLFIMG
                     LSTFSIGLLPTYERIGVLAPMLLFLLRIIQGIGLGGEWGGASLMVLEHA"
BASE COUNT         1202 a         1873 c         2011 g         1247 t
ORIGIN      
        1 aagaagctgc tcgcaatcag agttgccgcg tttgatctga agagaaaaag cactggattt
       61 atcggcacaa agtcgatggc agtctcgctg tcgtgatgca gtgaaatgca attctcccgc
      121 ttgaactcgg agagagtgga aattcgctgc gcgatgccgc gtttgaagta tgatcgtcat
      181 ctcttgagga gatggccgct gtgccagctt cgatcgagca cggttgcgcg cctcgacagc
      241 gaggagcgcc ggagacgtgg cgcgcccgac gccgtcgacc tcgatcgcgc caaatgcaca
      301 aaaatgtgaa cgttcacgcc tgccttcgga gccagacttt ccgatggtaa ccggccgctg
      361 ccgaaaggta tcggtgcttg tcccggtctt tcctgcggcc gtgcatgtac ctgcgcggcg
      421 gatggatcgc gaactgccgg tgaggccagt cgccgatcgg aaggcccgct caattgaccg
      481 cgctgcgtcc gtggtccggg atggctgcaa ggtcccggct tcgggcgatc cccgggtcga
      541 aaaagttgtg ttgcgccatc gcaaagacgc cgcccggcaa gtatggtaac gtcactggat
      601 acgggaggat gacgagcatg gctgaaccaa aactggaagt ccctgccgaa ttgcgcgatt
      661 tggccgaaaa gacgatcgac caggctgaga aggcgttcgg gatgtttttc gatgctgcgg
      721 gcaagtcgat ctccgccctc ccgggtgcgg gatcggatat ctccaagcag gcgctgacgt
      781 tcaccgaaca gaacatgaaa gccgccttcg agcatgcgcg aaaacttgtt cacgcgaccg
      841 attttcagga ggcgatgcgc attcagtcgg agttcctgag aagccagttc accaacgcgg
      901 gtgagcatat gaggcagatt acaggccagg tcatgtcggc cgccaaggac gcctccaaga
      961 gcaagttcta gggggatgct gcactgccca caaaatggtc ttgcgtggaa cggcgcggcg
     1021 gatttcaatg ggattgcagg tccatgacgg gccaccgggt ggattacccg ccgtctgtca
     1081 tcctttctgc gcatccctgt cggccaaaag ccggcaggga tttgcgcttg agggcattac
     1141 tttttgagcg acccgatata ggcggcgaga tcggccgcct cgctgcggct tagcggaaag
     1201 ctcggcatct tcgggtgcgg gtccagcaag aaaaacgcta tcttttccgc ggtgaaatcc
     1261 gacctgcgcg caatcgaggc gaaggacgga acatcggcac tggcctgtct ttgctcactc
     1321 cccacgacat ggcaggtggc gcaccagcgt ttggcgagat cggcgccgtg atcggcatcg
     1381 gcggccatcg ctcgcgaagc caaaggcgcc gcggcggcca tcatcagaat aagaaggcat
     1441 ttcaaacttg ccgtcatcag gcgatccccg tcagcgatgt tgcaaatcgt agcacatgct
     1501 aggtcattcg cccgggttcg cggttgatcg gcgtcaaaag ccgtttcgcg ggttgaaggc
     1561 tatcaatagt cgccggggtt ggcgatcatc agttgggtcg tgtttgccgg cgcgagatcg
     1621 ctggagccgc cgtccctgag gaatttttca agggaggcct ggatcttctc tttgagtgca
     1681 tcgggcccgc ggtcgctcag atcggtatgc agcgcgccgg tatttatgat gtcgatgccg
     1741 ccggtcttgg cctgggtcgg tgtgggcagg acgccaggat cggtcttgaa gttcggatcc
     1801 ttcgagcgaa aggagaggat cttcagcttg ttggagagca cgaacggcac gcgcaaatta
     1861 tcgagcgtga tcaccttcga caccagctca ggatgctgct tggcgacata catcgacacg
     1921 tcgccaccgt tggaatgtcc caccagtgtc aagtgatcgt agtaggcgtt cggctgcagc
     1981 ttctgaagtt cgccgagcac aaacaggata ttggcttcgc agcgcatata aaccccgcgc
     2041 cggcccacat agggcaaacc aaccttggtc atcagcggcg ggtcgctcgg cagatcctgc
     2101 tggatgctcg cgacgagata gccgcgtgcc gcaaggacat tggcaaggaa ggaatattcg
     2161 gtgttcttga cggtattgcc gttgctgatg atggcgatgg gaagcttcca gaggcccagg
     2221 ttggccttca cctcgtagtc gcggcgtacc gcgacgtcga cggcaacggg gcgttgccgc
     2281 gcggcgtcgt atagattgag cgtctcgtgc cggatagcca atttgctgat ggccaaatat
     2341 tcgccagcgg cgagcgcgca cagacaggcc aggatcgcaa atgcccttct catcgtttcc
     2401 aaacccgatc acttctattc aagagatggt gttggaacgc tcaaactgcg cccccggtat
     2461 ccgggtctcg aaattaaatt ttggaaagct cggtgggaac tgggaatatc cagctactga
     2521 ccaggatcgg ttaacgattc actgcagcgt ggcgtcgggc tcggttcggg cgggtacacc
     2581 cttcagatcg ttagcatgca agtgatttgc cgtccgggca gtcaggaatg ccaagtaaga
     2641 gccacgcgat gcgaaaattt gtttatttct ctttttcaga aatcaagatt ttcaacgccg
     2701 ttccgcgcta gcgccggcgt gccaccacga cacccagaag gaatgctccg aacaggctcg
     2761 tcagcggggc tcgacgggtg gcgcgccgaa tcgccgggat cacccgaccc gattgtacct
     2821 cggcggccac cgcgctcagc cgctcaacgg cggctttcag actggcagtg acctctccga
     2881 ccgtgcgcga cacgtcagtc gcagtgtcca cggctcgctc ggtcatgctc ggttgcagcg
     2941 tcggttccga tgcttcgaag tccactggcg atgccctcgc aagataatgc atgaggttgc
     3001 caacctttgg ctatcctcgc gtgcgcttgt ttttgaacag cgggtccaga ggacctttgg
     3061 ctatccgcga caggcgccgt ggtgaacggc gggttggcga cctcagggcg agaaatgccg
     3121 ggcggcttat tttgttcccc gattcacagg ccgtttcgac cggaccgaaa cggtcggtga
     3181 gaatctctgt tactttcgcg agccgcaaca tcgcaagggg cgagtcgtga acaatgcctt
     3241 cgggggggaa cccgcgcagc ggatagcagt gctgggggct cccatcgata tgggagcctc
     3301 gcagcgtggc accctgatgg gacccgcggc gcttcgaacg gcgggtcttt tgaccctgct
     3361 cgaaagcctc ggattcgacg tggccgacta cggcgatctg tcgatcgcgg ccatggccga
     3421 tctcgccgat ccgccgccca agaaggcgaa ccactaccgg gaaatccagc gctggacccg
     3481 cgcgttgagt cggcgcggct atgagctcgc gcaaacaggc gcaatcccga tctttctcgg
     3541 cggcgaccac tcgctgtcga tgggttcggt gaacgccatg gcgcggcatt ggcaggggca
     3601 ggggcgggag ctgttcgttc tgtggctcga tgcgcacgcc gactacaaca ccccgacgac
     3661 cacgctgacg gccaatatgc acggaatggc agcggcgttt ttgtgcggtg aagacggcct
     3721 cgagactctg ctcggcgagg agccgcgcgc ctcgatcggg cccgatcagc tcaagctgtt
     3781 cggtacgcgc tcgatcgaca agctggagaa agatcttttg cgcgcgcgct gcgtcagtgt
     3841 cgcagacatg cgggagatcg atgagttcgg tgtcggggtg ccgatccgcc gtttcatcga
     3901 caaggtgcgc ggccgggatg gcgtgctgca tgtcagcttc gatgtcgatt tcctcgatcc
     3961 tgccgttgcg cccggcgtcg gcaccacggt gccgggaggc gcgacctatc gtgaagcgca
     4021 tctggtgatg gaaatgctgc acgattccgg attggtccga tcggtcgaca tcgtggaact
     4081 caatcccttc ctggacgagc ggggccgcac cgcgcggacc gccgtcgagt tgatcggcag
     4141 cctgttcggt cagcagatca cagaccggcc gacgcccagc aatgcgatcg agccgggcga
     4201 gtaggccgat ggcgccaggc cttgatcacg gtgcgggctt gatgcccaaa tcggcgaaat
     4261 atcgattgag cgcgtctgcg tgaccgctct cgtctgcaag tgccacgtaa gtgtcgggcc
     4321 ggatcagata gagcgcatcg cgcgccagac ccgccgacag atgcgcgtcg cgccatggaa
     4381 aaacatgaag cggtacattg cgtgccgcgc accacgccga cagttcgcct ttcgccgaac
     4441 catagacatg aacttgccaa tccatgctgg tgagaggctt gaagttgtcg gagccggcga
     4501 ctgcgaccca tggcaggcga tctccggcgt gaacctggcc ggctgcacct cggcttaatg
     4561 ggccgccgcg gtagttcaac atcgtctgcg aaaccgtgcg aaaggcgaag cgccgcaccg
     4621 cttcgaacga gaatgccgcg ggaataacga cgggcgcgac gcgggtgcga atgaaatccg
     4681 caagccggcc ttcaccggtc gcaaaggtga agatgcgatc ggtggttgca acgagtcggt
     4741 gcgcaaagcc gatccgttcg gcctcatagc tgtcgagcaa gacgtcggag ccgcgtcctt
     4801 tgaggaccgc ggtcagcttc caggcgaggt tgatggcgtc accgatgccg gtgttcatgc
     4861 cctgtccgcc cgctgggcta tggatatgtg cggcgtcgcc tgcgaggaag acgcgacccc
     4921 tacggaaatg ctcggtgacg cggtggtgca catgataggt cgaaaaccag ttgaccttgg
     4981 cgaccatcac cttcatgtgg ttgatggcac gatcgctgac gtcggcgaaa gtgagtgttt
     5041 ccgcgcgctg agcgcgttct ccgcgcatcg ttccgatcag ccgtgcacgg ccctgtccgg
     5101 ccagcggaaa tacggccagg aaatccgcct cgtcgagatc gacatgcaac tcgccgttga
     5161 gcgcggggcc tgcggcatcg acgtcggcga cgtagaagac ttgcggatag gtgccgccgg
     5221 gaaagccggc gccgatcgtc tcccgcgcct tcgagcgggc gccgtcgcaa ccggcaagat
     5281 agatcgcgct gcaatcgtgc tcgctaccgt cggggccgcg caaatgggcc gtcacgccat
     5341 cgtcgtgctc ggcgaagcgg agcagttcgg tttcccgctc gacagtcaca ccgagtgctt
     5401 ccagtcggga gatcaggagc ttttcgtgct cgtcctgcgg gaagatttgc atgaaggggt
     5461 agggcgtcag ccccgcgccc agcgcattga gcgagatccg ggctcttcgt ttgcccttga
     5521 cccagaaatt gcccgccggg acttgatgac ccctcgcaat gacggcgtcc gcaaggtcaa
     5581 gctgccggta gagttcgagc gtgcgggcct gcaccgccag cgcgcgggaa gtggtcccgg
     5641 gctcggcagt cttgtccacg atacggaccc tggtgccgag cttcgtgagc caaagcgcga
     5701 gcaccaatcc ggtcggcccc gcaccgacga tcagcacctc ggtcgatgcc atctgtccct
     5761 ccattgacgt gggcctctca ataaacgcgc ggattcccgc ctcgatccat aatcggtcgc
     5821 ctccctccgg catggcgctt gcatcccgat atccggactt cttgcatccc gggagccatt
     5881 aatgaccatc acggcaactg cgccagccgc gacgagcgag cgtaccaaga tcaactccat
     5941 cgtctttgca agctgcctcg gcacgatcat cgaatggtac gatttcctga tctatgcgac
     6001 cggcgcagcc ctcgtcttca acaaggcgtt ctttccgacc tttgatccgc tcgcaggcac
     6061 gctggcggcg ctcggcagct acgctgtggg ctttctggcg cgaccgctcg gtggcgccct
     6121 gttcggccat ttcggcgacc ggttagggcg caaatccatg ctggtgctga cgctgttcat
     6181 catggggctg agcaccttca gcatcggcct gctgccgacc tacgaaagga tcggggtgct
     6241 cgcgccgatg ctgctgtttt tgcttcgcat catccagggc atcggtctgg gcggtgaatg
     6301 gggtggcgcc tcgctgatgg tgctcgagca tgc
//
LOCUS       DATLJT010001400         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_580214,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001400 DATLJT010000000
VERSION     DATLJT010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580214"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1704)
                     /locus_tag="VM910_32115"
     CDS             complement(46..1704)
                     /locus_tag="VM910_32115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HVI67187.1"
                     /translation="MVRLGFIIGLIALIGILLSGLAAYRVHEQELTIERIALARAIDV
                     HASLVQDRLTERELLARVAAGLFRMPSVIKANMLEPLRSSIYAFKTDFVVAGWVARLK
                     PNELDAARSELTNAGFPDPTIRNLDGKPLDARSLQQPIDVLMDVEPRNRQTVPFVGRA
                     LNDNPVFSTMLSLAMGEGKPYASDPLPLFRPDGQVGVVLAAPVTPPDATSPAGFVTFA
                     YELAPLMLANDDLSLFSVVLKDPRTLGGELLADDRGVVTTRVVGSDWPAPSSTRTVSF
                     GNRDWFLSYYAKTNAAKRAEQTAAIVGAIGLALTGIICGLFGYVASNNLRLSREIEVR
                     IGFERRLTAVIDELNHRVKNILAVIQSIVTRTLRHGADIDVARELLIGRIHAMSHVVS
                     LLSESQWQGVKLRGLFEARAIPHADRIAVSGPDIAVSARAAQSLSLLFFELASHSDEG
                     LSLVGKHPHIVANWDVTNSETDSTFTFRWEEFNTSEATRRPDSDFGLILLDRVAPEAL
                     GGTSKRYFTDVSYVYELTAPMETVVDMTERDRTEQFAAPVRPTR"
     gene            complement(2062..>2425)
                     /locus_tag="VM910_32120"
     CDS             complement(2062..>2425)
                     /locus_tag="VM910_32120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVI67188.1"
                     /translation="ADMRAKFPNLDLVLIESGGDNLAATFSPELADLTIYVIDVAAGD
                     KIPSKGGPGITRSDLLVINKIDLAPHVGASLEKMETDARRMRGERPFVMSNLKKSQGL
                     DRIIGFIEEKGGLVPRAA"
BASE COUNT          476 a          732 c          796 g          421 t
ORIGIN      
        1 agaccgtgcg cgcggtgatc ggcagcaacg gctagcgcgt gtgcgtcaac gcgtcggccg
       61 caccggtgca gcaaactgct cggtgcggtc gcgctcggtc atgtcgacga cagtttccat
      121 cggcgcggtg agttcataaa cgtaagagac gtcggtgaag tagcgcttgg acgttccgcc
      181 gagcgcttcc ggtgcgacgc gatccagcag gatcaggccg aaatcgctgt ccggtctgcg
      241 cgtcgcctcg ctggtgttga actcctccca gcggaacgtg aaggtcgaat cggtctcgct
      301 gttcgtaacg tcccagttgg cgacgatgtg cggatgcttg ccaaccagcg agagcccctc
      361 gtcggaatgc gacgcgagct cgaaaaacag cagcgacagg ctttgcgcgg cgcgagcgct
      421 gacggcgatg tcgggcccgc tcacggcgat gcggtcggca tggggaatgg cacgggcctc
      481 gaacaggccc ctcagcttca cgccctgcca ctggctttcg ctcaaaagcg acaccacgtg
      541 cgacatcgcg tgaatgcgtc cgatcaacag ttcgcgggcg acatcgatgt cggcgccgtg
      601 gcgcaaggtt cgcgtcacga tcgactggat cacggcgagg atgttcttga cccgatggtt
      661 cagctcgtcg atcacggcgg tgaggcggcg ctcgaaaccg attctgacct cgatctcgcg
      721 gctgagccgc aggttgttgg atgcgacgta gccaaacaac ccgcagatga tgccggtcag
      781 cgccagtccg atcgcgccga cgatggccgc cgtctgctcc gcgcgcttgg ccgcgttggt
      841 cttggcgtaa taggacagga accaatcgcg gttgccgaag ctcaccgttc gcgtcgacga
      901 cggcgccggc cagtcggatc ccaccacgcg cgtcgtgacc acgccacggt cgtcggcaag
      961 cagctcgccg ccgagcgtgc gcggatcttt cagcaccacg gagaacagcg acagatcgtc
     1021 gttggcgagc atcagaggtg cgagctcata agcaaaggtc acgaatcccg ccggcgaggt
     1081 ggcatcgggc ggcgtcaccg gtgcggcaag cacgacgccg acctgtccgt ccggacgaaa
     1141 cagcggcagc ggatcggaag cgtagggctt gccttcgccc atggcgagcg acagcatggt
     1201 gctgaacacg ggattgtcgt tgagcgctcg tcccacaaac gggacggttt gcctgttgcg
     1261 gggttcgaca tccatcagca cgtcgatcgg ctgctgcagc gaccgcgcat ccaatggctt
     1321 gccatccaga ttgcggatcg tcggatcggg aaagccggcg tttgtcagtt cgctgcgggc
     1381 ggcatcaagc tcgttcggct tcagccgagc aacccagccc gcgacaacga aatcggtctt
     1441 gaaggcataa atcgaggagc gcagcggctc cagcatgttg gccttgatca ccgacggcat
     1501 gcgaaacagg ccggcggcaa cgcgtgcgag cagctcgcgt tcggtcaggc gatcctgaac
     1561 cagactagca tggacatcga tggcgcgggc gagggcaatg cgctcgatcg tcaattcctg
     1621 ctcgtgcacc cgataggccg cgagcccgga gagcaaaatc ccgatgagcg caatcaatcc
     1681 tataatgaaa cccagccgaa ccacacgcct actcgagaat tggaggttgg cggaagagcg
     1741 ggaaaggcag gagagccatc gagagcccgc cgcaggggca atgggagaaa ccgcaatcag
     1801 gcccagcggg gcgatatgat ggtgatgcca tggtcggtac gcaacagccg tcggtgaggt
     1861 taaaccgcac tgcttaaggg gttcgggcgg cacaccccgg cacggattaa ccgccgtgga
     1921 gcgattttgt tccgggaggg gtgcaaagat tttcctcggc cgagcggccc ggcgttcata
     1981 atcgttaacg caaggatccg gaaagcatga tccggaagat aggcaccggt attccctcgc
     2041 tacaagcgcg gagatcttgc tctaggcagc gcgggggacg agtccgccct tctcctcgat
     2101 gaagccaatg atacggtcga gcccctggct tttcttgaga ttggacatga cgaacggccg
     2161 ctcgccgcgc atccgccgcg catcggtctc catcttctcg agcgacgcgc cgacatgggg
     2221 cgcaaggtcg atcttgttga tgaccagcag atcggaccgg gtgattccgg gcccaccctt
     2281 ggatgggatt ttgtcgccgg ctgccacgtc gatgacgtag atcgtcagat cggccaactc
     2341 cggcgagaag gtcgccgcca ggttgtcgcc gccggattcg atcaatacca ggtcgagatt
     2401 cgggaatttt gcgcgcatgt cggcg
//
LOCUS       DATLJT010001401        10417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_580239,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001401 DATLJT010000000
VERSION     DATLJT010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10417
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580239"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="VM910_32125"
     CDS             complement(<1..2026)
                     /locus_tag="VM910_32125"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI67189.1"
                     /translation="MQELTALLEGLEHRGADSVALGSIRSAVNRLQLNLEALDKLIAD
                     RMVVSELRRGHVRNALITHGDSQSLLIPWLQIVEGEIAQSRRIVDDTRLGADQRAAAG
                     SRLVASTASYHALQRVQFLITSISDRLQQIAAAEDADTLRVQVFRIQQSLREARGTTV
                     NLDSRLQPLLTGKLDEFGGQLAGISSIPELRLQELSIIANATQHLTENAALSRDLTQA
                     ADRLVSLATYDIAQANEDALSVQRFSATVLIAAVALSLLSSILIVWLYVGRSIVSRLT
                     ALSRSMLAIAEGNLTASLPTGGSDEIAEMGRVVEVLRKNTLERDELLIERAQAADRLE
                     KQVQERTAELAQSVKELRALGEVSQAVNSTIDLQTVLSTIIATAVQLSGTEAGTIYVF
                     DEASQEFQVHASYGMDEDLIAAVKGRRIRLGETMVSQAALQRKSMQLPDAEQDASSLV
                     LDVIQRAGFRALLTIPLLGADRIVGALVVRRKKPGEFPQGTIDLLETFAAQSVLAIQN
                     ARLFHEIEEKSHELEAASEHKSRFLASMSHELRTPLNAIIGLTEMMVTNTARFGTEKA
                     LEPLNRVHRAGSHLLGLINQVLDLSKIEAGKLELSPELVNLAPLIDEVIGTARQLADQ
                     NKNRLVVDAQENLGVLTVDPMRLRQILLNLLSNACKFTKEGEVKLAARKVA"
     gene            complement(2407..3501)
                     /locus_tag="VM910_32130"
     CDS             complement(2407..3501)
                     /locus_tag="VM910_32130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI67190.1"
                     /translation="MDRRQFVAGAGVGAAAAASAFPMPAIAQGVRELKMVTIWPKTLP
                     GLQSGAERVAEAITSLSGGRLNVKVFAAGQLVGAFESFDAVSSGLADMYHASEITWAG
                     HSPAFSYFCNVPFGFTPPEINAWIYHGGGQALWDELSAGFGLKPFMCGNTGPQMGGWF
                     TKELTGVESYKGLRYRMPGVGGEVLRRLGAVVVNLPAGEIIPSLRSGAIDASEFAGPW
                     VDMALGLHKAAKYYYYPGFHEPGAAISLAVNKQLWDGLGRSERSVIATAAAAENAVSY
                     ADFTANDAIALQQIANDPAIEVRKLDDSILQALGRISGEVLYETSRKDDLTRRVYDGF
                     IKFRISALRWGDISERAFLNARALPYPFGG"
     gene            4172..5848
                     /gene="ilvD"
                     /locus_tag="VM910_32135"
     CDS             4172..5848
                     /gene="ilvD"
                     /locus_tag="VM910_32135"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HVI67191.1"
                     /translation="MAKLNKRSQLITAGIARSPNRAMLRAVGFEDGDFEKPIVGVANG
                     HSTMNPCNAGIQPLVDRVMVGLKEAGAMPQVFGFPTVTDGIGMGTEGMKYSLVSREVI
                     ADAIEVAVNGQRMDGVVAVGGCDKNMPGAMIAMARMNVPGIFVYAGTIKPGLWKGEKL
                     TIVSPFEAVGAFTAGKMSREDFDGIEKNACPSVGACGGQYTANTMSSSFEAMGMSLLG
                     SSQMASPAREKADSAAESARVLVEAIRADLKPRDIITRKSIENAVALVMATGGSTNAV
                     LHYLAIASAAGVEWTIDDFERVRRKVPVLCNLKPSGQYVATDFHSAGGVPQVLKILLI
                     NGVLHGDCMTVHGKTMAEMLRDVPSEPRKEQKVIHPWSSPVYKQGHLAILKGNLSPEG
                     CVAKITGLKQTSITGPARVFDSEPECLRAILDKKIKSGDVVVIRYEGPKGGPGMQEML
                     APTSALVGQGLGASVGLLTDGRFSGGTWGMVVGHVAPEAYEGGLIALVREGDSITIDA
                     KKRLIQLNVPAKELARRKRLWKAPKPRHTTGVLGKYIKLVSTASRGAITD"
     gene            complement(6072..7115)
                     /locus_tag="VM910_32140"
     CDS             complement(6072..7115)
                     /locus_tag="VM910_32140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI67192.1"
                     /translation="MATGAAFSFPAPAIAQGLRQLKMVTAWPAGSPGLQSSAERLAQR
                     ISTASDGRINIRVFPAGAFVGPFETFDAVGTGVADMYHASDYYWEKKSPALNFFAAVP
                     FGFTANELFAWVQYGGGQELWDALSGQFNIKPLLCLNTGSQMGGWCTREVPSPDAFRG
                     LRYRMPGLGGEVLRRLGASVVTVPGGEIVQSLESGAIAGGEWVGPWLDMDLGLHKVAV
                     YYYYPGFDEPGTGSTLGINKRLWESFDASDRRLIEDATAAEYTRSLAEFDFNNALWLR
                     RLRDEGNVKILKFDDSVLKELYRISKDVVAEAGSGDDLSKKIYTSYQQSFSLIMDWSH
                     IAEGAVLNSRRLT"
     gene            complement(7371..7496)
                     /locus_tag="VM910_32145"
     CDS             complement(7371..7496)
                     /locus_tag="VM910_32145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67193.1"
                     /translation="MMRRRAFITLFGGTAVAWPLAAARATASAAGDRVLEHPVIR"
     gene            7746..7925
                     /locus_tag="VM910_32150"
     CDS             7746..7925
                     /locus_tag="VM910_32150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67194.1"
                     /translation="MTRGKGDKPVHTHDEQWARPHEERLHALLNERLEGYVDVGVGAG
                     FEYDQSPADCASSVL"
     gene            8266..8490
                     /locus_tag="VM910_32155"
     CDS             8266..8490
                     /locus_tag="VM910_32155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67195.1"
                     /translation="MATTGIDICSPYGMACTECNDLLVAPKWSAYVSKREVRHFWSCE
                     NCGHEIEMTVNPRINTASKLSQGVEWSLVG"
     gene            complement(8763..9392)
                     /locus_tag="VM910_32160"
     CDS             complement(8763..9392)
                     /locus_tag="VM910_32160"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVI67196.1"
                     /translation="MLERERREDQVDRLVIMPSATGRSGRGDQSRFAGRIMKSPREVL
                     IRPIALSLAAILLTTALVAEAYSHATALHIRDPEDLALAFLLPTIFITVFFDSTLAVA
                     SSFVCGLAAAYVIYPPQTSIWIDDPTHVAELGFIVLLAITASKAVGVIVDGKPLGSSG
                     ARAESQQCSLACNPGNAFLRSPSHRAHHVGTSHSRRTGPRDRMSAVPFD"
     gene            complement(10218..>10417)
                     /locus_tag="VM910_32165"
     CDS             complement(10218..>10417)
                     /locus_tag="VM910_32165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67197.1"
                     /translation="VPVEFFFEGAPNASAPHGSDGSTLSMAEINDFISDSDGLRLIRA
                     FMRIDNRPAAQDRDTRARNCR"
BASE COUNT         2128 a         3147 c         3104 g         2038 t
ORIGIN      
        1 tggccacctt gcgtgccgca agcttgacct cgccttcctt ggtgaacttg cacgcattgc
       61 tgaggaggtt gagcagaatc tgccgcaacc gcatgggatc gacggtcaat acgccgaggt
      121 tctcttgggc atcgacgacg agcctattct tgttctgatc cgcgagttgc cgcgccgtgc
      181 cgatgacctc atcgatgagc ggcgccaaat tcaccaactc ggggctgagc tcgagcttgc
      241 cggcctcgat cttcgagagg tcgagcacct gattgataag tccgagtagg tgcgagccgg
      301 cgcggtgcac gcgattgagc ggctctagcg ccttttcagt gccaaaacgc gccgtattag
      361 tgaccatcat ttcggtcagc ccaatgatgg cattcagcgg cgtgcgcagc tcatggctca
      421 tgctcgcgag gaaccgcgat ttatgctcac tcgccgcctc gagctcgtgg cttttctcct
      481 caatctcgtg gaacagtcgc gcgttttgga tcgccaacac ggattgtgca gcaaaggtct
      541 ccagcaaatc gatggtgcct tggggaaatt cacccggttt cttgcggcgg accaccagcg
      601 caccgacgat ccgatccgcg ccgagcaatg ggatggtcaa gagggcgcgg aagccagcgc
      661 gctgaatgac atcgaggacg agtgacgagg catcctgctc ggcgtccgga agttgcatcg
      721 atttgcgctg gagcgccgcc tggctgacca tcgtctcgcc aagccggatg cgtcgaccct
      781 tgactgccgc gatcaaatct tcatccatcc catagctggc atgcacttgg aattcctggc
      841 tggcttcatc gaacacatag attgtaccgg cttccgtccc cgaaagctgg accgccgtgg
      901 caatgatggt ggagagcacg gtctgcaagt cgatcgtcga gttgaccgcc tggctgacct
      961 caccgagcgc tcgcaactcc ttgaccgatt gggccagctc agctgtgcgc tcttgcacct
     1021 gcttttcaag acggtcggcc gcttgtgctc tctcgatcag caactcgtca cgttccaagg
     1081 tgtttttgcg caagacctca acgactcgcc ccatttctgc gatctcgtcg ctaccgccgg
     1141 tcggcaggct tgccgtaaga ttgccctcgg caatcgccag catgctgcga ctgagcgctg
     1201 tcagccgaga gacgatgctg cgccccacat agagccagac gatcaggatt gagctcagca
     1261 gactgagcgc gacagccgct atcaggacgg tcgcgctgaa tcgttgaacc gagagtgcat
     1321 cttcattggc ctgcgcgatg tcgtacgtgg caagtgagac caggcgatcc gccgcctgag
     1381 tgaggtctcg cgacagcgca gcgttctcgg tcaaatgctg cgtcgcattc gcgataatgc
     1441 taagttcctg caatcgcaac tcggggatac tgctgatgcc cgcgagctgt ccaccgaatt
     1501 cgtcgagctt gccggtcaag agcggctgca atctcgaatc caggttgacc gtcgtcccgc
     1561 gcgcctcgcg cagggattgt tggattcgaa acacctgtac gcggagggtg tcggcatcct
     1621 ctgcggcagc aatctgctgc agcctgtcgc ttatggacgt gataaggaac tgcacgcgct
     1681 gcaatgcatg atacgatgct gtcgaagcga ccaatcggct gccggccgct gctcgttggt
     1741 cggcacccag ccgagtgtcg tcgactatcc tacgcgactg tgcgatttcg ccctcaacga
     1801 tctgcagcca cggtatcaaa aggctttggc tatcgccgtg ggtaatgagc gcgttgcgca
     1861 catggcctcg ccttagttcg ctcacgacca tgcgatccgc gatcagcttg tcgagcgctt
     1921 caagattgag ttgcagacgg ttgacggccg atcgtatcga gccaagcgca acgctgtcgg
     1981 cgccgcgatg ctctagaccc tccaatagtg cggtaagttc ctgcatctcg gccgcgattt
     2041 tccgagagct ttcggcatga tcggccgccg tcgcggcggc gagcaaggcg ggagcggctg
     2101 aagcgattcg ctcagcttgt cgcgatactt cctgcaaggc cagtgctgca gggacccgcc
     2161 tcgccgtgat gcgatcaagg acatcgccga tttcgcgaaa cgagtacatc gcgacacccg
     2221 caccgatcac tcctaatgcg ctaatcccga ggaaagccaa aagcaatcgg ctcgccacac
     2281 cgagccgcga tgatgcccat gcccgcgctt tcggggtctg ggacgccgcc ggaaggctag
     2341 acctcaccag gttcatgtct gcgcctttgg caccgacgtt tgacgaacgc cggtcgcgta
     2401 caggcatcat cctccgaacg ggtaaggtag ggcgcgcgca ttgagaaagg cgcgttccga
     2461 aatgtctccc cagcgcaggg ccgagatccg gaacttgatg aagccgtcgt agacccgccg
     2521 cgtcagatcg tccttccggc tggtttcgta gagcacttcg ccggatatcc tgccaagcgc
     2581 ctgcagaatc gaatcgtcga gcttgcggac ctcaattgcc ggatcattgg caatctgctg
     2641 gagcgcgatg gcgtcgttgg cggtgaaatc cgcatatgaa accgcgttct cggcggccgc
     2701 cgccgtcgcg atgacgctgc gctcgctacg accgaggccg tcccacagct gcttgttgac
     2761 tgcgagcgaa atcgccgctc ccggttcgtg gaagcccggg tagtagtagt atttggcagc
     2821 cttgtgcagg cccagcgcca tgtccaccca gggcccggcg aattcgctcg catcaatcgc
     2881 gccggaccgt agcgacggga tgatttcccc tgccggcagg ttgaccacta cggcgccgag
     2941 ccgccgtagc acctcgccgc ccacgccggg catgcgatag cgcaggccct tgtaggactc
     3001 cacgcccgtg agttccttgg tgaaccaacc gcccatctgc ggaccggtat tgccgcacat
     3061 gaacggcttg aggccgaacc ctgcgctcaa ctcgtcccac agcgcctgcc ctccgccgtg
     3121 atagatccac gcgttgattt ccggtggcgt gaagccgaac ggaacgttgc aaaagtacga
     3181 gaatgcgggg gagtgcccgg cccaggtgat ttccgaggcg tggtacatgt cggcaaggcc
     3241 ggacgagacc gcatcgaacg attcgaacgc gccgaccagc tgaccggctg cgaatacctt
     3301 gacgttcagc cgaccaccgg agagcgacgt aatggcctcc gcaacacgct cggcgccaga
     3361 ctggaggccc ggcaaggtct tgggccagat ggtgaccatc ttgagttcgc gcacgccttg
     3421 ggcgatcgcg ggcattggaa aagcggatgc tgcggccgca gccccgacgc cagcgcctgc
     3481 aacgaattgg cggcgatcca tgtttctctc cccgttagtt gtcaggcctg tcctttagtt
     3541 tctctgaaag aggcattcgc agcaaaataa cgtgcgtgtc gccactcgga tggaggggtc
     3601 agctggcacg cctctgagtg tgatcggcga agcggttgtg ccccgccgac aggattaggc
     3661 cccctctttc atgcatgatt caggacacga tactgacccg ataaaaggat caagcttata
     3721 aataccatat ctcgagccgc gggtcttcaa agtacttcgc actcgaaggc gcccaatggt
     3781 ggcgatgtgg cacaactttg aagtgtctcg gagcacaaac gaattctggt ttcagcagtt
     3841 ggtttagcgc ggctccccta tttgggtgtg taatttactt cacacatctc aacgagcaat
     3901 ttttggacga gttctgtccg tcacgccggc acccccgcgg aaggccctgc gcgtcaatga
     3961 gtacatggcc tagtcggcac tttgcgccgg atgtaccctc gcctgtgcgg ttcactccag
     4021 gcgtggagcg cattcgccga atgttagctc ggcaccactg gcgtccctca ccacttgggc
     4081 gatgtgcgcg gcatgcatga ttgttaataa ggggtcgaca ggcgacaaat ctagagttct
     4141 tgtcgttttg caattctggg agggatatga ggtggcaaaa cttaacaagc gttcccagct
     4201 catcaccgcg ggcatagccc gctcacccaa ccgcgcgatg ttgcgcgccg tgggcttcga
     4261 agacggtgac tttgaaaaac ccattgtcgg tgtggcgaac ggccactcta cgatgaaccc
     4321 ttgcaacgca ggcattcagc ctctcgtcga ccgcgtgatg gtgggcctca aggaggcggg
     4381 agcgatgccg caggtcttcg gctttccgac ggtgaccgac ggcatcggca tgggcaccga
     4441 aggcatgaag tattcgctgg tatcgcggga agtcattgcc gacgcgatag aagtcgcggt
     4501 caacggccaa cgcatggacg gtgtggttgc ggtcggtggt tgcgacaaga acatgccagg
     4561 cgccatgata gcgatggcac gcatgaacgt gcccggtatc tttgtatatg ctggtaccat
     4621 caagcctggg ctctggaaag gcgagaaact cactatcgtg agcccgttcg aggcggtggg
     4681 cgccttcact gcgggtaaga tgtcacggga ggacttcgac ggcatcgaaa aaaacgcctg
     4741 cccgtctgtg ggcgcctgcg gcggccagta caccgcaaac acgatgtcct catcgttcga
     4801 agcgatgggc atgagtctgc tcggctcatc gcagatggca tcgcctgccc gcgagaaggc
     4861 agactctgca gccgagtctg ctcgtgtgct tgttgaggcg atccgggcag acctgaaacc
     4921 gcgcgacatc atcactagaa aatcgatcga gaatgcagtc gcgcttgtca tggcgactgg
     4981 cggctcgact aacgcggtgc tccactatct ggccatcgca agcgccgcag gcgtggaatg
     5041 gaccatcgat gacttcgaac gcgtgcggcg caaagtgccg gtgctatgca atctcaaacc
     5101 gtcgggccag tacgtagcga cggatttcca ttctgccggt ggcgtcccgc aggtgctgaa
     5161 gatcctgctc ataaatggcg tcttgcatgg tgactgcatg actgtgcatg gcaagaccat
     5221 ggccgagatg ttgagagatg ttccttcaga gccgcggaag gaacagaagg tgatccatcc
     5281 gtggtcgagt ccggtttaca agcaagggca tcttgcgatc ttaaagggca acctttcgcc
     5341 cgaaggctgc gtcgccaaga tcacggggtt gaaacaaaca tcaatcaccg gccctgcacg
     5401 cgtgttcgat tctgaacccg aatgcctgcg ggcgatcctg gataagaaga tcaagtcggg
     5461 cgatgtggtc gtgatccgct acgagggccc aaagggcggg cccggcatgc aggaaatgct
     5521 tgcgccaacg tctgccctgg tcggacaggg gcttggcgca tcggtaggcc ttctcaccga
     5581 tggacggttt tccggcggaa cctggggaat ggttgtcggt catgtcgcac cggaagctta
     5641 cgagggggga ttgatcgcac tagtgaggga aggtgattcg atcacgattg acgctaagaa
     5701 gcgccttatt cagctcaacg ttccagcaaa agaacttgcc agacgaaaga gactatggaa
     5761 ggcgcccaag cctcgccata ctactggcgt gctcggcaag tatatcaagc tcgtctcgac
     5821 cgccagccgc ggggcgatta ccgactgatt attcgtccct gcccattagt atcgatgatt
     5881 gttgcccgcc cgccgcgagc ggccacaccg ccgagcagcg tgatgaactc gcggcatccg
     5941 tgtactcaaa aatccgcttc ctgtgagcac ggctcagtca atcttcgctc tgaaatcgac
     6001 cgaagtgctg ttgccacgca ctgaccgcga tccggttccg atgttgctgc tgatgatgcg
     6061 ataaaaaccg attacgtcag ccgccggcta ttgaggacgg cgccttcagc gatgtggctc
     6121 caatccatga tcaacgagaa agactgctga tagctcgtgt agattttctt ggaaaggtcg
     6181 tcgccggagc cggcctcggc gaccacgtcc ttgctgatgc ggtacaactc cttcagcacg
     6241 gagtcgtcaa acttcaggat cttgacgttg ccttcgtccc gcagtctgcg cagccacagc
     6301 gcattattga agtcgaactc cgcgagggag cgcgtgtatt cggccgcagt tgcgtcttcg
     6361 atgagccgcc ggtcactcgc gtcgaaactc tcccacaagc gcttgttgat gccgagtgtt
     6421 gagccggtgc ctggctcatc gaatccggga tagtaatagt agacagcgac cttgtggaga
     6481 cccagatcca tgtcgagcca gggccccacc cattcgccgc cagcgatggc gccggactcg
     6541 agcgactgca cgatttcgcc tcccggcaca gttaccacgc tggcgcccag ccttcggagg
     6601 acctccccgc cgagccccgg cattcggtag cgcagacctc tgaacgcgtc tggcgagggg
     6661 acttcgcggg tacaccagcc gcccatctga gaaccagtat tgaggcagag aagcggctta
     6721 atgttgaatt gcccgctgag tgcgtcccac agctcttgac caccgccata ctgcacccac
     6781 gcgaacagtt cattcgcggt gaagccgaac gggacagctg cgaagaaatt gagtgccggc
     6841 gacttctttt cccaatagta atcgctcgcg tgatacatat cagcgacacc ggtcccgacg
     6901 gcgtcgaacg tttcgaacgg gccaacgaac gccccggccg ggaacacccg gatgttaata
     6961 cggccgtcgc ttgcggtgct gatccgctgc gccagacgtt ccgcgctgga ttgaaggcct
     7021 ggcgaccctg cgggccaggc ggtcaccatc ttcagctgtc gtagtccctg agcgatcgca
     7081 ggcgccggaa agctaaaggc cgcgcccgtg gccaatgatc ccgcaccggc caaaatatcc
     7141 cgccgcctca tagctgcctc ccatctgtac ctgctggtgc ccttaaaatg tggcgcgaca
     7201 ccaaaaacat tcgaccatag gccgaggttt atcgcaaacg gcgaccccaa atgcccgaat
     7261 cggtcatttt cgccagatcg gccggctgtt gacgctctcg gcatgtccgc ttccttcggc
     7321 gaagccgcgt tcggctaagc tctggcggtg agactaggag ctgctcatcc ctatcggatg
     7381 accggatgct caagaacccg atcaccggca gcgctggctg ttgcgcgcgc cgccgcaagt
     7441 ggccacgcga ccgcagtgcc gccgaacagc gtgatgaacg cgcgacgcct catcattcga
     7501 tcacctcgtc ggcgctggcg agcagcgtcg gcggaacatc aaggccaagg gctcgggcgg
     7561 tcttgaggtt gatcaccaac tcaaagcggg tcgctgcacc accagaagct cggatggttt
     7621 ttcgcccttc aggatacggc ccgcgtagat gccaatctgg cgatagcctc ggccgtgctc
     7681 gacgcgtagc tgacgaggcc gcctgcacca accagctcgc gacgggagta catggcgggg
     7741 agtgaatgac gcgcggcaaa ggcgacaagc ctgtccatac gcatgacgaa caatgggcca
     7801 ggccccacga ggagcgcctg cacgcgctgt tgaatgaacg cctcgaaggc tacgtcgatg
     7861 tcggcgtcgg tgctggcttt gagtatgatc agtcgccggc cgactgcgcg tccagcgtcc
     7921 tttaactgca atacggaagg tctcggggcc gatgagagcg ccgatcaaga cggcgttggg
     7981 cgccagctcg gccaattcct tgacagggcc gaatgcactg aatttccgtt gctccgcaat
     8041 cgcggccttg agccgctcgc cgtgacgcat tgcctattgc actcgcactg ccaatggcac
     8101 ggcagcgcaa gcgggagcgg cggccgcctc tttttcatcg ctggcgactg gcaggatttc
     8161 cgtcttttgc ggcagcgcac cattcatcta caagttgacg ctcaaagtgt cataatgctt
     8221 ttatctcccc acgccataag aaaccaaaaa taaagggagg tggccatggc gaccactggg
     8281 attgacattt gctcgcccta tggaatggcg tgcaccgaat gcaacgacct gctggttgca
     8341 ccgaagtggt cggcgtacgt gagcaaacgc gaagtccgcc acttctggtc ttgtgaaaat
     8401 tgcggtcacg agatcgagat gacggttaat ccgcgtatta acaccgcatc gaaactcagc
     8461 cagggcgttg aatggtcgct cgtcgggtga gcagtcacgc tcggcggtga gcgttcagga
     8521 acccgatcac cggcatcgcc gagtagcgcg aggaacgcgc gggggtcagt tggacattga
     8581 ccttcctcct tcccgctctg gcggcaagag agaccagata gatgcgccgc catgcgctcg
     8641 gcttacatcg acggtaagcc aagctcaccg cacccggaac cggccaagaa aaagccgcgc
     8701 cctgaggagc gcggcgcaaa aagagccccg ccggggcgag gctcagattg gcaggtgacc
     8761 acttaatcga acggtaccgc actcatgcgg tcacgcggcc ccgtccgacg actgtgacta
     8821 gtaccgacgt ggtgtgcgcg atgtgatggc gatctcagga atgcgttccc ggggttacac
     8881 gcgaggctac attgctgcga ttcagcacgg gctcccgatg accccaatgg cttgccgtcc
     8941 actatgacac cgaccgcctt gcttgccgtt atcgccaaca acacgatgaa tcctagctcg
     9001 gcaacgtgag tcgggtcatc aatccaaatg ctcgtttgcg gcgggtagat aacgtaagca
     9061 gcggcgagtc cgcatacgaa ggaacttgcg accgcgagcg tgctgtcaaa aaatactgtg
     9121 atgaagatcg tcggcaacaa aaaggcaagt gctaaatcct ccggatcgcg gatatggagg
     9181 gccgtcgcat gggaatacgc ctcagctaca agcgctgtcg taagcaggat cgcggccaag
     9241 gacagcgcaa tggggcgaat caacacctcg cgcgggcttt tcatgatccg accggcaaaa
     9301 cgcgattggt caccgcgccc gctacggccc gtagcgctcg gcatgatcac cagcctgtca
     9361 acttgatctt cacgccgctc gcgctccaac atcgccagcc cgtgccgtcc caacgattta
     9421 gactgtacat gagcaggagc ggcgaagcga ggcggcgcgc tcaaaatggc gcgttttgct
     9481 ggcgccgaaa gggccacgcc gccagatgct cgagcgccgg caccatctgc ctaacaatgg
     9541 gcagcacaga aagaagcccc accggcgagc ggggcttaag gcaataccgc agtctcccct
     9601 taacgtcaca gattgataaa cggccgcccg tgacgtgccg aagggcttgt ctttcgccaa
     9661 aacgagaacc ccgcccgggc gtgtagggca tggcgccggt tgcccgcacc actttgaacc
     9721 tggtgcgctc aacaggcgac caacgggccg aggggacggc tctgagaagc ggagcgcatt
     9781 atgtctgatg agttcgacgg cctggacgcg accgagaagg ctggcattgg agccgcgctg
     9841 attaggtgtc gcgccctcgc ggccaagatt gcgaatgaac tccgtaccaa gaggcccgag
     9901 cacttcgtcg acaagttgtc gctggcagac gttcacgcct cccttgagcg cgctgccgat
     9961 gtccttagca cgatgaagag ctcgcaggcg cggggtcgtc agtggcaccg ataattgaga
    10021 ggattttcgt ctacccacgc tttcgcgggc gtgacatggg cctttcgaga gcagaagcgg
    10081 caaactcaag ataaaacaga agctctgccg ccgatgagac attgacgatg ggcttttcgg
    10141 cagaaagccc ggcattccag cgtcgatttc cttgagcacg attgcagccc tcagcaagct
    10201 tcccctagtt gccgtcgtca ccggcaattt cttgcacgag tgtcgcgatc ctgcgccgca
    10261 gggcgattat caattcgcat gaacgctctg atcagcctta agccatctga atcggagatg
    10321 aagtcgttaa tctcggccat cgataacgta cttccatcag agccgtgtgg cgctgacgcg
    10381 ttcggcgcgc cctcgaagaa gaactccacc ggcacct
//
LOCUS       DATLJT010001402        10991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1497999,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001402 DATLJT010000000
VERSION     DATLJT010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497999"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VM910_32170"
     CDS             <1..868
                     /locus_tag="VM910_32170"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HVI67198.1"
                     /translation="KLRKLEYIIPDAIASNADTLVSIGGVQSNHTRLVAAVAAKLGMK
                     CCLVQESWVPHEDAVYDRVGNILLSRVMGADVRLVDEGFDIGIRRSLEEAIADVKARG
                     GKPYGIPAGASVHKYGGLGYVGFAEEVRAQERELGFAFDYIVVCTVTGSTHAGMLVGF
                     ARDGRERRVIGIDASFTPAQTKAQVLAIAQNTARLVELGREIVADDVVLLEDYAHPAY
                     GVPSDATKEAIRLCARLEGMITDPVYEGKSMQGMIDLVRKGFFPDGSKVLYAHLGGAP
                     AINGYAYTFRNG"
     gene            complement(1055..1807)
                     /locus_tag="VM910_32175"
     CDS             complement(1055..1807)
                     /locus_tag="VM910_32175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI67199.1"
                     /translation="MRTPWPGFKARVSNRLARHFRSTPLRLSGKAPMVSFTFDDVPRS
                     AATVGAPMLEEYGGRATFYVAGSLVGQRSEHGDGIGPDEIVALHHRGHEIACHTFSHR
                     RAVELDATSMAAEIAQNRRCLEGLDASIRLENFAYPYGLASIARKAQLSSTFRSSRGI
                     LPGVNSGIIDLQFPRATPLINRDIDDDGVERAFAETEATGGWLIFYGHDVAATPSPYG
                     CTPQLLRHALEAARRRKLPIVTVADALRKVGA"
     gene            complement(1816..2823)
                     /locus_tag="VM910_32180"
     CDS             complement(1816..2823)
                     /locus_tag="VM910_32180"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HVI67200.1"
                     /translation="MTLLFDKSRSIASPALAPAQDASGHACQNELTIALINNMADSAL
                     KATERQFMRLLKAAAGKRRVQFHCFSLPSVARSQAARNHIDREYGDIADLGDLRIDGL
                     IVTGAEPVARDLPEERYWHELTEIIDWAKTNTRSTIWSCLAAHAAVLQLDGIERRRLE
                     RKCSGVYDCAKVTDDWLTNGLPASLKVPHSRHNEIRERDLVAHGYQILTRSNVAGVDV
                     FARQFQSRFIFFQGHPEYDASSLQREYMRDLARFLAGDRDDYPEIPAGYFDPTTVEQL
                     TSFESRARTQRDPLLAAELPGLTLRHDIAPGAAAGVIFANWLAFLGEDARAKQHATSR
                     R"
     gene            complement(2858..4144)
                     /locus_tag="VM910_32185"
     CDS             complement(2858..4144)
                     /locus_tag="VM910_32185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HVI67201.1"
                     /translation="MRNETIAIHAGYDPDPTTHAVAVPIYQTVAYAFDSADHGAALFN
                     LEAEGFRYSRIANPTTSVLEKRIAELEGGVGALAVATGQAALHFAFVNLADGGGNIVS
                     VPQLYGTTHTLLGHVLPRQGIAGRFAESDQPDAIEKLIDENTKAVFAETIGNPAGNIC
                     DIEALAKVAHRHGVPLIVDNTVATPILLKPFDYGADIAVHSLTKFLGGHGTTLGGAIV
                     DSGSFPWDRHANRFPAFNQPDASYHGLVYAERFGPKAFIERARSVYQRTMGSVLSPFS
                     AFLLLQGIETVALRVERHVKNAREVAEFLRNDPRIAWVNYAGFPDSPYYALAQKYLAG
                     NASSLFTFGIDGGLEAGKTFYDSLKLITRLVNIGDAKSLACHPASTTHRQMSSEQQRM
                     AGVLPETIRLSIGIEHVSDIIEDIDQALAKACGRPS"
     gene            complement(4485..5420)
                     /locus_tag="VM910_32190"
     CDS             complement(4485..5420)
                     /locus_tag="VM910_32190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67202.1"
                     /translation="MPLDPIAKRLLAMMAAAAPESRTRPSPGERRDSLKKLMQFARAE
                     AASVTTVDGTMPGPAGEIAYRLYAPAVEAASAAPGFVFFHGGGMVAGSIQTHDRIAAA
                     LAEATGCRLISVDYRLAPEHKFPAAVEDAIAATEYVARNAEAFGIDAERLVVGGDSAG
                     ATLAAVVCQHVQRHAGLRISAQCLICPVLDFEETSPSREAFAENYLIDRTTLQADLAD
                     YLPDNADPADVRISPLRALNLKGLPTAIIHTAEFDPMRDEGNAYARKLLAAGVVVEHI
                     CHDGMIHNFHAMGAVLPQARLILSQIGEQIRRAVS"
     gene            5574..6461
                     /locus_tag="VM910_32195"
     CDS             5574..6461
                     /locus_tag="VM910_32195"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HVI67203.1"
                     /translation="MSYIVKALSYSQTWTYFEGDWHDGNAPIMGPRTHAAWLGSTVFD
                     GARAFEGVAPDLDRHCARVNWSAGNFGLKPVVDVESWIALAREGIARFAPNAELYIRP
                     MYWAQHGSGGGVLFDPETTNWCLCIYEAPMPQPTGSAITLSPFSRPTMECAPVDAKAA
                     CLYPNNSRALIEAASRGFNNCLMLDMLGNVAEFGNANVFMAKDGVVFTPVPNGTFLNG
                     ITRQRVIDLLRGDGVTVIEATLRYDDFKSADEIFSTGNFAKVAPVIRIDDRQLQPGRF
                     YTRARKLYWDFAHAMKLAA"
     gene            complement(6492..7373)
                     /locus_tag="VM910_32200"
     CDS             complement(6492..7373)
                     /locus_tag="VM910_32200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA acyltransferase"
                     /protein_id="HVI67204.1"
                     /translation="MTALKPLIRCREIGESDIDRVADLLTRGFFGRSRDYWMQGLLRQ
                     SRREVPQGYPRFGYMLDNDGAPVGALLLLYSLREVGGEMAVHCNLSSWCVEPAYRNYA
                     PMLTKIAQRHNEVSYFNISPANWTWPIIEAQGFCAYCKGLFFSFPLLSSVAPGTTVEI
                     VPADATEVDGLAPCDVRLLARHAAYGCLSLVCRTRNGVFPFILQQIRIRRGWFAPPAM
                     QLIYCRDVADFKACAGAIGRLLLSRGKISVLLDANGRVPGLIGLYTEKRGRKYFRGPH
                     RPRLADLTDTELVVYGP"
     gene            complement(7398..8378)
                     /locus_tag="VM910_32205"
     CDS             complement(7398..8378)
                     /locus_tag="VM910_32205"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HVI67205.1"
                     /translation="MNTPIRTESPELAPQAADPLDHLAEILFYKMGSDGVYARTALYE
                     DVVERLAALISRHREPDTEVMRFPPVMNRAQLEKSGYLKSFPNLLGCVCGLHGTEREI
                     NAAVSRFDAGGDWTGSLSPADLVLSPAACYPVYPIAASRGTLPAGGLRFDVAADCFRR
                     EPSRHLDRLQSFQMREYVCIGSPDDVSAFRGRWMARAQAIARDLGLTFRVDYASDPFF
                     GRVGQMKAVSQKQQSLKFELLVPLRSEDKPTACMSFNYHRDHFGTTWDIRDADGAPAH
                     TGCVAFGMDRLAVATFHTHGTDPSRWPSRVRELLGIEPHASVDQHVEGWR"
     gene            complement(8417..9640)
                     /locus_tag="VM910_32210"
     CDS             complement(8417..9640)
                     /locus_tag="VM910_32210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI67206.1"
                     /translation="MNVQETNFLIADDRGHALLANHGPSFQERTATVASVAAAEADDV
                     DQKARFPKAAIDAARERKILGAQIPVAQGGFGASIFDVAEMCYALGRACASTAMIFAM
                     HQTKVACLVRHGSGSRFHEALMRRVASEQLLLASSTTEGQNGGNVRSSAAAIERGDAQ
                     ISLVRSATVISYGAEADGIVTIARRAGDAASSDQVLLAVTRDHYTLERIQGWETLGMR
                     GTCSAGFELKINGPAEMIFPEAYEKIHAQTMTPVAHLCWSSAWAGVAAAAVERAQRFV
                     RKAARGAGGQMPPGAAHFTAAKMSLAKLRAMIATNLDSYAAREYDERALTSLDFQSSI
                     NLLKVEASELAVQTAMSAMRACGLAGYRNDGEFSVGRHLRDVLSSPLMINNERILGNI
                     ATASLMSGIPTSLRD"
     gene            9948..10220
                     /locus_tag="VM910_32215"
     CDS             9948..10220
                     /locus_tag="VM910_32215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HVI67207.1"
                     /translation="MQSQNADVRTRITALVKVILEQNAISAEVTPEALLVDVGLTSVD
                     MVNLMLGVEAEFDFTIPQDEITPENFQSVETLQRMVLREMGAVQAA"
     gene            10384..>10991
                     /locus_tag="VM910_32220"
     CDS             10384..>10991
                     /locus_tag="VM910_32220"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160353.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     5'-deoxy-5'- methylthioadenosine (MTA) to adenine and
                     5-methylthio-D-ribose-1- phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HVI67208.1"
                     /translation="MTKAVLGIIGGSGIYDLPGLQDVREEAIKSPWGEPSSPVRHGTI
                     AGLPIVFLPRHDKGHRLSPSDINYRANIDVLKRAGVTELVSLSACGSFKEELPPGMFV
                     LVDQFVDRTYKRESSFFGKGCVAHVSMAHPVSPRLRIHLAAAAEAEGIAIARAGTYVC
                     MEGPQFSTYAESMTYKNLGYSVIGMTNMPEAKLAREAEICYAT"
BASE COUNT         2078 a         3248 c         3610 g         2055 t
ORIGIN      
        1 caagctgcgc aagctcgaat acatcatccc ggacgcgatc gcctccaacg ccgacacgct
       61 cgtgtcgatc ggcggcgtgc agtccaacca tacccgcctg gtcgcggcgg tcgcggcgaa
      121 gcttggcatg aaatgctgcc tcgttcagga aagctgggtg ccgcatgagg acgccgtgta
      181 cgaccgcgtc ggcaatattc tgctgagccg tgtgatgggc gccgatgtcc gcctcgtcga
      241 tgaaggtttt gacatcggca ttcgcaggag tttggaagaa gcaatcgcgg acgtcaaggc
      301 caggggcggc aagccctatg ggattccggc cggcgcgtcc gtccacaaat atggcgggct
      361 cggctatgtc ggtttcgccg aagaggtgcg ggcgcaggag agagagctcg gctttgcgtt
      421 cgattacatc gtggtctgca ccgtcaccgg ctcgacccat gccggcatgc tggtcggctt
      481 cgcccgggac gggcgcgagc gccgggtgat tggcatcgac gcgtcgttta cgccggccca
      541 aaccaaggcg caggtgcttg caatcgcaca aaatacggcg cgcctggtcg aactgggcag
      601 ggagatcgtc gccgatgacg tggtgctgct cgaagactat gcccatccgg cctacggcgt
      661 tccgtccgac gcgaccaaag aagcgatccg gctttgcgca cgactcgagg gcatgatcac
      721 cgacccggta tatgagggaa aatcgatgca gggcatgatc gatctggtga ggaagggctt
      781 ctttccggac ggatcgaagg tgctctacgc ccatctcggc ggcgcaccgg cgatcaacgg
      841 ctatgcctat accttccgca acgggtgagc gcctccggcc ggtcgcgaac gcaataacac
      901 cgaggttgtg aggatcgacg ccgcaccaac ctttttcatc aacggcagga tatacaaggg
      961 caatatgccc tttgatcaga tggagggaat catcaggtcg cagctacgac gatagtcgcc
     1021 gggccaccgg aatctgacgg acgatggagc aaccttacgc tccgactttt cgcagggcgt
     1081 ccgcgacggt tacgatcggc agcttgcgtc gcctggctgc ttccagcgcg tggcgcaaga
     1141 gctgtggcgt gcatccgtag gggctcggcg tcgccgcgac gtcgtgaccg tagaagatta
     1201 gccagccgcc ggtcgcttcc gtctccgcga aggcgcgttc gacgccgtca tcgtcgatat
     1261 cccgattgat cagcggcgtc gcgcgcggga attgcaggtc gatgatgccg gaattgacgc
     1321 cgggcagaat gccgcgcgac gagcggaaag tgctgctcaa ttgcgccttg cgcgcgatcg
     1381 aggcgagccc ataagggtaa gcgaaattct caagccggat cgaggcgtcg agtccctcga
     1441 gacaacgccg gttttgtgcg atttccgctg ccatgctcgt tgcatcgagc tcgacggcgc
     1501 gccgatgcga aaaggtgtgg caggcgatct cgtgtccccg atggtgaagg gcaacgatct
     1561 catcgggccc gatcccgtcc ccgtgttccg agcgttggcc gaccaggctg cctgcgacgt
     1621 aaaaggtggc gcgtccgcca tactcctcca gcatcggtgc gcccacggtc gccgcactcc
     1681 tgggaacgtc gtcgaaggtg aagctcacca tcggagcttt gccggacagt cgaagcggcg
     1741 tgctccggaa atgccgggcg agcctgttgg aaacgcgcgc tttgaacccc ggccacggcg
     1801 tgcgcatgcg atgggtcacc ttcgtgacgt ggcgtgttgt ttggcgcgcg cgtcttcgcc
     1861 gaggaaggca agccagttgg cgaagatcac accggctgct gcgccgggtg cgatgtcgtg
     1921 gcggagtgtg aggcccggaa gctcggccgc cagcagcgga tcgcgctgcg ttcgtgcgcg
     1981 gctttcgaaa ctggtgagct gctcgaccgt cgtcgggtcg aaatagcctg cgggaatttc
     2041 gggatagtcg tcgcggtcgc cggcaagaaa tcgcgcgaga tcgcgcatat attcccgctg
     2101 cagcgacgag gcatcatatt ccggatggcc ctggaaaaag atgaagcggc tttggaactg
     2161 ccgggcgaag acatcgacgc cggcaacatt ggaccgcgtc aatatctgat agccatgcgc
     2221 gacgaggtcc ctctctctga tctcgttgtg tcgcgaatgt ggtaccttga gcgaggcggg
     2281 caggccattg gtcaaccagt catcggtgac cttggcgcaa tcgtagacgc cggagcattt
     2341 ccgctcgagc cgccgtcgct cgatcccgtc caactgcagc accgccgcat gggctgccag
     2401 acatgaccag atcgtcgacc gcgtatttgt cttcgcccag tcgatgattt cggtcagttc
     2461 gtgccaatac cgctcttccg gcaaatccct tgcgaccggc tcggcaccgg tcacgatgag
     2521 gccgtcgatg cgcaggtcgc cgagatcggc gatatcgcca tattccctgt cgatgtgatt
     2581 tcttgccgcc tgcgaacgag ccaccgacgg tagcgagaag cagtgaaact gaacgcgccg
     2641 tttgccggct gccgctttca ggagacgcat gaactgccgc tcggtcgcct tcaaggcgct
     2701 gtcggccatg ttgttgatca gggcaatggt cagttcgttt tggcaggcat gcccggacgc
     2761 atcctgcgcg ggcgccagcg cggggctcgc aatcgaccgg cttttgtcga acaggagcgt
     2821 catgccgatc cagtccgatc tattccgccg cctcgagtca cgacggccgg ccgcaagcct
     2881 tggcgagggc ctggtcgatg tcctcgatga tgtcggagac gtgctcgatg ccgatcgaca
     2941 ggcggatcgt ttccggcaga acgcctgcca tgcgctgctg ttcgctcgac atctgccggt
     3001 gcgtggtgga cgccggatgg caggccagcg acttggcatc gccgatattg acgagacgtg
     3061 tgatcagctt tagcgagtcg tagaaggtct tgcctgcctc caggccgccg tcgatgccga
     3121 aggtgaatag cgaggacgcg ttgccggcca gatatttttg ggccagcgcg taataggggc
     3181 tgtccgggaa gcccgcataa ttgacccagg cgatgcgcgg atcgttgcgc agaaactcgg
     3241 cgacctcacg cgcattcttg acatggcgct cgacgcgcag cgcgaccgtt tcgatgccct
     3301 gcagcagcag gaatgcgctg aagggcgaca gcaccgaacc catggtgcgt tggtagacgc
     3361 tgcgcgcgcg ctcgatgaag gccttcggtc cgaagcgctc ggcgtagacg aggccgtgat
     3421 atgacgcgtc gggttgattg aaggcgggga agcggttggc atgccgatcc cagggaaagc
     3481 tgccgctgtc gacgattgcg ccgcccagcg tggtgccgtg accgcccaga aacttggtca
     3541 gcgaatgcac ggcaatgtcg gcgccgtagt cgaacggctt gagcaggatc ggggtcgcga
     3601 cggtgttgtc gacgatcagc ggcacgccgt gccggtgcgc aaccttcgca agcgcctcga
     3661 tgtcgcaaat gttgcccgcg ggattgccga tcgtttcggc gaaaactgcc ttggtgttct
     3721 cgtcgatcag tttctcgatg gcatcgggtt ggtcgctttc ggcgaaacgg ccggcgatgc
     3781 cctgacgcgg caggacatgg ccgagcagcg tatgcgtcgt gccgtagagt tgggggacgg
     3841 aaacgatgtt gccgccaccg tcggcaaggt taacgaaagc gaaatgcagg gcggcttggc
     3901 ccgtcgcaac cgcgagcgcg ccgacgccgc cttcgagctc ggcaatgcgc ttttccagga
     3961 cggaggtggt ggggttggcg atgcggctgt aacgaaagcc ttcggcctcg agattgaaca
     4021 aggcggcgcc atggtcggcg ctgtcgaaag cataggcaac ggtctgatag atcgggacgg
     4081 cgacagcgtg ggtggtcgga tcggggtcgt agccggcgtg aattgcgatc gtctcgttgc
     4141 gcatcgtcga cctccagcca gcgccgccaa ttcgaagttc ctgcagcgca agctgcaaat
     4201 tgctgcaagg gagcacgaac ttcggaaagc gggaccaggc gcaccgtttt ttgcgttttg
     4261 cgccgcagtc ttgattagag gcgaggtgtg aagcgagcaa taattcgcgc caacatcgat
     4321 gaaataaccc taatgaaagg ccgccgattg attaaagttt gatcgatttt ccagatgcgt
     4381 catggcgagc gcaagtgaag cgatgcatgg ccacgaagaa gccggattgc ctcgtcgccc
     4441 gcgttcctcg ctatgacaac acgagacgat gcgacattcc gtcgtcaact cactgcgcgc
     4501 ctgatctgct cgccgatctg cgacaggatc agcctggcct ggggaagcac ggcgcccatg
     4561 gcatggaaat tgtggatcat gccgtcgtga catatgtgct ccacaacgac gcccgccgcg
     4621 agcagcttgc gggcataggc attgccctca tcgcgcatcg gatcgaactc ggcggtgtgg
     4681 atgattgcgg tcggaaggcc cttgagattc agcgcgcgga gcggtgaaat cctgacgtct
     4741 gcggggtcgg cgttgtcggg caggtagtcg gcgagatcgg cttgaagcgt cgtgcggtcg
     4801 atcaggtagt tctccgcgaa ggcctcgcgc gagggcgagg tctcctcgaa atcgagcacc
     4861 ggacagatca ggcattgtgc cgatatcctg aggcccgcat gccgctgcac atgctggcag
     4921 accacggcgg ccagtgttgc accggcggaa tcgccaccga ccacgagcct ttcggcatcg
     4981 ataccgaaag cctctgcgtt gcgtgcgacg tactcggttg ccgcgatcgc atcctcgacc
     5041 gctgccggga acttgtgctc gggcgcaagg cgatagtcga ccgagatcag ccggcagccg
     5101 gtcgcttccg ccagcgccgc ggcgatgcgg tcatgggtct gaatgctgcc ggcgaccatg
     5161 ccgccgccat ggaagaacac gaagcccggg gcggcggatg ccgcttcgac ggccggcgca
     5221 taaaggcgat aggcgatctc gccggccggg cccggcatcg tcccatccac ggtcgtcaca
     5281 ctggcggcct cggcacgcgc gaattgcatc agcttcttga gcgagtcgcg ccgttcgccg
     5341 ggagagggtc gtgttcgcga ttccggcgcg gccgcggcca tcatggcaag gagacgtttg
     5401 gcaatcggat cgagcggcat ggcggttccc ggttaaccaa ccgagcttac catggctgcg
     5461 gatcaaccgt gaaccggccg cgacggtccg cccgaggtga aatcatttaa gaaattgcgg
     5521 caatcatgcg cggttggaag cggcctccgt cggccgcggc atggatggcg gacatgtcct
     5581 atatcgtcaa agccttaagc tactcgcaga cctggaccta tttcgaaggc gattggcacg
     5641 acggcaatgc gccgatcatg gggcctcgca cgcatgcggc ctggctcggc tcgaccgttt
     5701 tcgacggcgc ccgcgcgttc gaaggcgtcg cgcccgatct tgatcggcac tgcgcgcggg
     5761 tcaattggtc cgcgggcaat ttcgggctga agccagtcgt cgacgtggaa tcctggatcg
     5821 ccctggcccg cgaaggcatt gcgcgatttg cacccaatgc cgagctttac atccggccga
     5881 tgtattgggc gcagcacggc agcggcggcg gcgtattgtt cgatccggaa actacgaact
     5941 ggtgtttgtg catctacgag gcgccgatgc cgcaaccgac cggcagcgcg atcacgctct
     6001 cgccgttcag ccggcccacg atggaatgcg cgccggtgga tgccaaggcg gcgtgcctct
     6061 atcccaacaa ttcgcgcgcg ctgatcgagg cggcctcgcg aggcttcaac aattgtttga
     6121 tgctcgacat gctcggcaat gtcgccgaat tcggcaatgc caacgtgttc atggcaaaag
     6181 acggcgtggt tttcacgccc gttcccaatg gcacattcct gaacgggatc acgcggcagc
     6241 gggtgatcga ccttctgcgg ggcgacggcg taaccgtgat cgaggcaacc ctgcgctatg
     6301 acgatttcaa gagtgccgac gaaatcttct cgaccggtaa ttttgccaag gtcgcacccg
     6361 tgatccggat cgacgaccgg cagcttcagc cgggacgctt ctatacgcgc gcgcggaaac
     6421 tctattggga ttttgcgcat gccatgaaat tggccgcgta aggtccgtca ttcccggata
     6481 ttggtacgcc gctacggccc gtagaccaca agctcggtgt cggtgaggtc ggcaaggcgc
     6541 ggccggtgcg gccccctgaa gtatttgcgg ccccgctttt ccgtatagag accgatcagg
     6601 ccgggcacgc gtccgttggc gtcaagcagg acggaaatct tgccacgcga cagcagcagc
     6661 cggccaattg cgccggcaca ggctttgaag tcggcgacat cgcggcaata gatcaattgc
     6721 attgcgggcg gtgcgaacca gccgcggcgg atgcggatct gctgcaggat gaacggaaag
     6781 acgccgttcc tggtgcggca caccaggctg aggcagccat aagccgcatg tcgcgccaga
     6841 aggcggacat cacaaggtgc cagaccgtcg acctcagtgg catcggccgg aacgatttcg
     6901 accgtcgtgc cgggcgcaac cgacgacagc agcggaaacg aaaagaacaa tcccttgcaa
     6961 taggcgcaaa aaccctgggc ttcgatgatg ggccaggtcc agtttgcggg gctgatattg
     7021 aaatagctga cttcgttatg gcgctgcgcg atcttggtca gcataggcgc atagttgcgg
     7081 taggccggct cgacacacca gctcgacaga ttgcagtgga cggccatctc gccgccgacc
     7141 tcgcgcaagg agtagagcag aagcagcgcg ccgaccggcg cgccgtcatt gtcgagcatg
     7201 tagccgaagc gcgggtaacc ctgcggcacc tcgcgtcgtg actggcgcag caggccctgc
     7261 atccagtaat cccgcgagcg gccgaaaaag ccgcgagtca ggagatctgc gacgcgatcg
     7321 atatcggatt cgccgatttc gcgacaccgg atgagtggtt tgagtgctgt catggcgata
     7381 gatcgcttga ggtgtggcta ccgccagcct tcgacgtgct ggtcgacgct cgcgtgcggc
     7441 tcgatgccga gcaactcgcg caccctggat ggccagcggg acgggtccgt gccgtgggtg
     7501 tggaacgtgg cgaccgcgag ccggtccatg ccgaaagcca cgcaaccggt atgggccggc
     7561 gcgccatcgg cgtccctgat gtcccaggtc gtgccgaaat gatcgcggtg gtagttgaag
     7621 ctcatgcagg cagtcggctt gtcctccgag cgaagcggca cgaggagctc gaatttaagg
     7681 gattgctgct tctggctcac ggccttcatc tggccgaccc ggccgaagaa cgggtcgctc
     7741 gcataatcga cgcggaaggt cagaccgaga tcgcgggcga tcgcctgcgc gcgagccatc
     7801 cagcgcccgc ggaaggcgga aacgtcgtcg gggctgccga tgcagacata ttcgcgcatc
     7861 tggaacgatt gcagccgatc gagatggcgc gaaggttcgc ggcggaagca atcggccgca
     7921 acgtcgaagc ggaggccgcc agccggcaac gtgccgcggc tcgccgcgat cggatagacg
     7981 ggatagcagg ccgccggcga cagcacgaga tcggcgggtg agagcgagcc ggtccagtcg
     8041 ccgccggcgt cgaagcggct gacggcggcg ttgatctcgc gttccgtgcc atgcaggccg
     8101 cagacgcagc cgagcaggtt cgggaaactc ttcaggtagc cggatttctc gagctgtgcg
     8161 cgattcatca ccggcggaaa gcgcatgacc tcggtgtccg gttcgcgatg ccgggagatc
     8221 agcgcggcga gccgctcgac cacgtcttca tagagagcag tgcgggcgta aacgccgtcg
     8281 gagcccatct tgtaaaaaag gatttcggcg aggtgatcca gcgggtcggc ggcttgcggg
     8341 gcgagttcgg gcgattcggt tcggattgga gtgttcatgt cgagcctctt ttgtatccgc
     8401 cgatacggtg ctcttgtcag tcgcggaggg atgtggggat gccgctcatc aggctggccg
     8461 tcgctatatt gccgagaatg cgctcgttat tgatcatcag cggcgaggaa agcacatcgc
     8521 gcagatgccg gccgacggaa aactcgccgt cgttgcgata gccagcgagg ccgcaggcgc
     8581 gcatcgcgct catggctgtc tggacggcga gctcggaagc ctcgactttg agcaggttga
     8641 tggaggactg gaaatcgagc gaagtgagcg cgcgttcgtc gtattcgcgt gccgcgtagc
     8701 tgtcgagatt ggtcgcgatc atcgcccgca attttgccag cgacatcttc gcggcggtga
     8761 aatgcgccgc acccggcggc atctggccgc cggcgccgcg tgcggccttg cggacgaagc
     8821 gctgcgcgcg ttcgacagcg gcggctgcaa cgccggccca ggccgacgac cagcagagat
     8881 gcgcgaccgg cgtcatcgtc tgcgcgtgga ttttttcgta cgcctccgga aagatcatct
     8941 cggccggacc gttgatcttc agctcaaagc ccgcgctgca ggtgccgcgc atgcccagcg
     9001 tctcccatcc ctggatgcgc tcgagcgtgt aatgatccct ggtgacggcc agcagcacct
     9061 gatcggaaga agccgcatcg ccggcgcgtc gcgcgatggt gacgatgccg tcggcctcgg
     9121 ctccatagga gatcacggtg gcgctgcgca ccagcgaaat ctgggcgtcg ccgcgctcga
     9181 tcgcggcggc gctggagcgg acgttgccgc cgttttggcc ttccgtggtc gaggacgcca
     9241 gcagcaattg ctcggaagcc acgcgccgca tcagcgcctc atgaaaacgg ctaccgctgc
     9301 cgtgtcggac caggcaggcg accttggtct gatgcatggc aaagatcatc gcggtcgagg
     9361 cgcaggcgcg gccgagcgcg tagcacatct cggccacgtc gaagatcgac gcgccgaagc
     9421 caccctgtgc gaccggaatc tgagcgccta gaatcttccg ctcgcgcgcc gcgtcgatcg
     9481 cggccttcgg aaaacgggcc ttttgatcga catcatcggc ctctgccgcg gcgacggacg
     9541 cgacagtggc tgttcgttcc tgaaaggagg gaccgtgatt tgccagcaaa gcgtggccgc
     9601 gatcatccgc gatcaaaaag tttgtttcct ggacgttcat gaccgacacg acctccgagg
     9661 ttttgtcgcc gcgagaacac gcgcttcgcg ttcagcgatc cttccgattg ttttcggatt
     9721 ggaatcggag gttatggatt taattcaaat gcgtcgatcg gattcagggt aaatgacttc
     9781 atttcgaaat cgacggttaa tctgtcgtat ttcgacagca tcgatttttc gcgaccgcgt
     9841 tagcgttaaa agcaaaagcg caatttgcgt cgaagccgta agacaattct tcaacggagt
     9901 ggcggttata gtcgtgccgg ctgtgatgcg agaagaaagg tttgaggatg caaagccaga
     9961 atgccgacgt tcgtacccgg atcactgctc tggtgaaggt tattctcgaa cagaacgcga
    10021 tatcggccga agtcacgccc gaagcgttgc tcgtcgatgt cgggctgact tcggtggaca
    10081 tggtcaatct gatgctcggt gtcgaagccg agttcgactt caccatcccg caggacgaga
    10141 tcacaccgga aaatttccag tcggtcgaaa cgctgcagcg gatggtactt agagagatgg
    10201 gcgcggtaca ggcggcgtga tgcaatgcgg tcattccggg atgtcggaag gaccggaccg
    10261 ggaatctcga gattgcgggt tcaatgctga cgcttcgtcc ggaacgcatc ctccccccat
    10321 ttcatggcat tccaaatctt cgctaaaact ggcatgactt agcccgaacg aagcggggtg
    10381 agcatgacaa aggcagtgct tggcatcatc ggcgggtccg gcatctacga tctgccggga
    10441 cttcaggacg tacgcgagga agcgatcaaa agcccatggg gtgaaccttc ctcacccgtg
    10501 cgtcatggta ccatcgcggg acttccgatc gtgttcctgc cgcggcatga caaggggcat
    10561 aggctgtcac cttccgacat caactatcgg gccaatatcg acgtgctgaa gcgtgccggc
    10621 gtgaccgaac tggtctcgct gtcggcctgc ggttcattca aggaagagct gccgcccggc
    10681 atgttcgtgc tggtggacca gttcgtcgat cgcacctaca agcgcgaaag ttccttcttc
    10741 ggcaaaggct gcgttgcgca tgtgtcgatg gcgcatccgg tgtcgccgcg gctgcgcatc
    10801 catcttgcgg cggcggccga ggcggaaggc atcgcgatcg cgcgcgcggg aacctatgtc
    10861 tgcatggagg ggccgcaatt ttctacctac gccgaaagca tgacctacaa gaacctcggt
    10921 tattccgtga tcggcatgac caacatgccc gaggcaaaac tcgcgcgcga ggcggaaatc
    10981 tgctacgcca c
//
LOCUS       DATLJT010001403         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2187112,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001403 DATLJT010000000
VERSION     DATLJT010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187112"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VM910_32225"
     CDS             complement(<1..445)
                     /locus_tag="VM910_32225"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67209.1"
                     /translation="MRRREFIRLFSSTVVAWPLTARAQQPPGKMRRLGVLLPGAPPDR
                     LVEAMQGRLGELGYSEGRGVAFEFRWAEGKLERLTQLATELADLKVDVITAFSTPAAV
                     AAQKATTTIPIVFTLVGDPVGTGLVSNLARPGGNVTGLSLLATELA"
     gene            1418..1930
                     /locus_tag="VM910_32230"
     CDS             1418..1930
                     /locus_tag="VM910_32230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67210.1"
                     /translation="MSDKKPSLIIDGTSKWIGPMWAQIDDNMILRYTPTKTQFTSGAQ
                     VVLDVSTLPMVVEELAKVPPEARRGPLIVNPRTGLPYRNSYFGEVWRRVRKIGGISKE
                     VWNRDMRAAAVTEARQAAAPTDDVAKLAGHSNKRTTARIYDRDRLQASRRVAQARVAH
                     RDTDGEQGVS"
BASE COUNT          405 a          652 c          635 g          433 t
ORIGIN      
        1 cagccaactc cgtagccagc agtgacaagc cggtcacatt tccgcccgga cgggcaagat
       61 tcgacacgag tccggtccct actgggtctc cgaccaacgt gaaaacgatc ggaatggtcg
      121 tggtcgcttt ctgcgccgct acggcggcgg gcgtgctgaa tgcggtgatt acatccactt
      181 ttaagtcggc gagttccgtt gccaactggg taagccgctc gagctttccc tccgcccacc
      241 gaaattcaaa ggcaacgccc cgcccttcgc tgtaaccaag ttcacccaat cttccttgca
      301 tcgcctcaac gagccggtca ggcggcgctc ctggcagcaa gacacccagg cgccgcatct
      361 tccccggtgg ctgctgcgct cgcgcggtca gcggccatgc aactactgtg ctgctgaaaa
      421 gtctgatgaa ctcgcgccgc ctcatgggat ttactcccct ggccgagaac caccttcgta
      481 aaagtctaat acgatcttgg agggaaaacc atgcaccgca tttctggacg atttgctgcc
      541 ttcgctgttc gggaagccat tgctgagcga ccagcccaat cgaagtcaaa tcgcgtggca
      601 ttgacggccg cccggaggtc ggacattcag tcttattcga ttatcgacga tacccgtcgc
      661 aatccggcgc ggcttcgtgt gtggtggcgc aggtggcttt tggtcaacca atgcccagct
      721 gatgcatcgg gaattcggca accctgcgag cgcaatggta gcaatggtag ctggagtgtt
      781 aacggccaag tctcttcgct ttgaacgctg tcactatccg agccgcgcca cgatgcgccg
      841 ccgtgcgctc ccgcttggtt gggcgcgggc cgagctttga agcgatagct gcttcaatct
      901 tcttgcggtc ttttggcgtg atgcggttgc tgaggcgatc aagacgctca agcatatgct
      961 cccaggtata gacccgctgg gctgaactct ttgcttgcct cgtcgagaag ctgccgagat
     1021 aggcgacaag gctctgtcgt ggcttgccgt tgacgcggac gctctcaacc aaaatggcat
     1081 tccaggtggt gtcgccaacc tggcgtagcg cccagtcgtt tcgctcggct cgtactctgc
     1141 tttgccatct gacgaacatg cgtagtgtat aatcagggta tcattgagtc gcaagttgta
     1201 cactacgcgg ccttaggtca aggcggtact gcgtgatatt ctcgccaaca ttcgttttgc
     1261 tgggccgcgc ccgcgcgtcg aggcgccgac cgcgaccgag atcatcgccg cgcgaaatgc
     1321 cgcgcacgga ttgggccacg cgcctgccgc gctcgcttat gcgctccaat ttgagggcgc
     1381 gatgcgccaa tgggacgtgg tcggtaaatg ggtgccgttg tccgacaaga agccgtcttt
     1441 gatcatcgac ggcacgtcta agtggatcgg cccgatgtgg gcgcagatcg atgacaacat
     1501 gatcctgcgc tatacgccga ccaagacgca gttcacctcc ggcgcgcagg tggtcctcga
     1561 cgtcagcacg ctgccgatgg tggtcgagga actggccaag gtgccgcccg aggcgcgccg
     1621 gggaccgcta attgtcaatc cgcgcaccgg cctgccgtac cgcaactcgt atttcggcga
     1681 ggtctggcgg cgagtgcgca agatcggtgg catcagcaaa gaggtatgga atcgcgacat
     1741 gcgcgccgct gcggtcactg aggcgcgcca agcggcagcg cccaccgacg acgttgccaa
     1801 actcgccggc catagcaaca agcgcaccac cgcgcggatc tatgaccgcg atcgcctcca
     1861 ggcctctcgg cgcgtggctc aggcccgcgt cgctcaccgt gatacagatg gcgaacaggg
     1921 tgtgtcgtaa tgtgtcgcgc cgcacaacaa aaacaatggc ttaaagtggt tccacattta
     1981 cagagcgtta accttccgta tcgcttaatc ggtacgatgc cgcttcgtgc atcggagcat
     2041 cgcgctccgg ccagcggcgg gggaagctgg tgggcaccgt catcaaattt ccggacggcg
     2101 agggacgcat tgtgcgcttc ggccg
//
LOCUS       DATLJT010001404         1707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4026697,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001404 DATLJT010000000
VERSION     DATLJT010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4026697"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VM910_32235"
     CDS             <1..355
                     /locus_tag="VM910_32235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67211.1"
                     /translation="TAFARLGNSGLIVTASPSSVVHRELIVALAARHKLPAVYWERFF
                     VTAGGLISYGPDQADQYRGAAGYANRILKGEKPADLPVQAPTKYELVINLNTAKALGL
                     DMPGSVLARADEVIE"
     gene            374..1351
                     /locus_tag="VM910_32240"
     CDS             374..1351
                     /locus_tag="VM910_32240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67212.1"
                     /translation="MKLPRRNFLHLAAGAAALPAVSRFAWAQAYPTRPVHIIVGFAPA
                     GGTDIMARLIGQWLSERLGQQFVIENRPGAASNIGTEMVVNAPPDGYTLLVATFVNAV
                     NASLYNKLNFNFIRDIAPVASIAREPFAIEVHPSVPVKTVPEFITHAKANPGKINMAS
                     AGIGSGNHVAGELFKMMTGVNLVHVPYRGAGPALVDLLGGQVQVMFASMSSSIEYVRA
                     GKLRALAITIATRSPVLPDLPTVAEFVPGYEFSFWTGIGAPRNTPAEIVDKLNTEINA
                     ALADPKIKARLAEWGATALPGSAADFGKLIAAETEKWGKVVKFAGVKAD"
     gene            complement(1521..>1707)
                     /locus_tag="VM910_32245"
     CDS             complement(1521..>1707)
                     /locus_tag="VM910_32245"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HVI67213.1"
                     /translation="MIEKRDGSYYVRDLRSTLGTIVNGEPIGDHFRGDDAPLRAGENE
                     VIAGGVDSPFVFSVFIG"
BASE COUNT          338 a          545 c          508 g          316 t
ORIGIN      
        1 cacggccttt gcgcgcctgg gaaatagcgg cttgatcgtg acggcgagcc cgtcgtcggt
       61 cgttcatcgc gaactgatcg tagcgcttgc ggcccggcac aagttgccgg cggtctactg
      121 ggaacgcttc ttcgtcaccg ccggaggcct gatctcctac gggcctgatc aggccgacca
      181 gtaccgtggc gcggccggct acgccaatcg catcctcaag ggcgagaaac ccgccgacct
      241 gccggtgcag gcgccgacca aatacgaact ggtcatcaat ctcaataccg ccaaggcact
      301 cgggctcgac atgccgggga gcgtgctcgc ccgcgccgac gaggtgatcg agtgatgtga
      361 agggaggttc aaaatgaaac ttccccgccg aaattttctc catctggccg caggcgctgc
      421 cgcgctgccg gccgtctcgc gcttcgcttg ggcgcaagcc tatccgacgc ggccggtgca
      481 catcattgtc ggatttgccc ccgctggtgg caccgacatc atggcacgcc tgataggaca
      541 atggctgtcg gagcggctcg gccagcaatt cgtcatcgag aaccgaccag gagctgccag
      601 caatatcggc accgagatgg tcgttaatgc gcccccggac ggatatacgc tccttgtggc
      661 cactttcgtg aacgcggtca acgccagcct ctacaacaag ctcaatttca atttcatccg
      721 cgacatcgcg ccggtcgcga gcattgctcg cgagcctttt gccatagagg tgcacccctc
      781 ggtgccggtc aagacggttc ccgagttcat tacccacgcc aaggccaatc ccggcaagat
      841 caacatggcg tccgccggca tcggttccgg aaaccatgtc gctggtgaac tgttcaagat
      901 gatgaccggc gtgaacctcg tgcacgtgcc ttatcgcggc gcgggccctg cgcttgtcga
      961 tctgctcggc ggacaggtgc aggtcatgtt cgccagtatg tcctcatcga tcgaatacgt
     1021 cagggccggc aaacttcgcg ccctggcaat aaccatcgcg acacgctcgc cggtgctgcc
     1081 ggacctcccg acggttgccg aattcgtgcc gggctatgag tttagcttct ggaccggcat
     1141 cggcgcgccg cgcaacacgc cagccgagat cgtcgacaag ctcaacacgg agatcaacgc
     1201 ggcccttgcc gatcccaaga tcaaggcgcg gcttgccgag tggggcgcca ccgcacttcc
     1261 aggctcggct gccgacttcg gcaagctcat cgccgccgag accgaaaagt ggggcaaggt
     1321 ggtgaagttc gcgggcgtca aagcggattg atcgggcaat cccggcaaat cttccataat
     1381 gtttcgttcc gcgaatcgcg gcgacgaagt gatggagtga agaggcgcga attcatcgtg
     1441 cgggtctgtt aggattgatt ggcaaaacga accagcggga ggagcgacag aaacccaaag
     1501 cctgccgccg aaccggcttc tcagcctatg aacacggaga agacaaacgg tgaatccacg
     1561 ccgcccgcaa tcacttcatt ctctcccgct cgcagtggcg catcgtcacc gcggaaatga
     1621 tcgccgatgg gctcgccgtt gacgatcgtt ccaagcgtgc tgcggaggtc gcgcacataa
     1681 tagcttccgt cgcgcttctc gatcatg
//
LOCUS       DATLJT010001405         5581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1957914,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001405 DATLJT010000000
VERSION     DATLJT010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957914"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1341
                     /locus_tag="VM910_32250"
     CDS             109..1341
                     /locus_tag="VM910_32250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HVI67214.1"
                     /translation="MTNSARLSLHIPEPKVRPGDQPDFSDLKIPAAGSVPRPDIAVRA
                     KELGAYADTLIRVLDDKGKAVGPWDPKLDPETLRRGLRAMMLTRAYDDRMFRVQRQGK
                     TSFYMKCTGEEAVAVGQALALENTDMCFPTYRQQGLLITRKWSMVDMMCQVFSNSRDR
                     LKGRQMPVFYSSKQAGFFSISGNLGTQYCQAVGWAMASALKGDTRIAAGWIGDGATAE
                     GDFHYALTFASVYRVPVILNIVNNQWAISTFQGVAGGEQATFAARALGYGLPGLRVDG
                     NDLLAVYAATKWAADRARANHGATVIELYTYRASAHSTSDDPSKYRPAKEEEKWPLGD
                     PIERLKQHLIVRGEWSADRHSAQEKEVVEEVTAAAREAESYGTLGNGPTHSASTMFED
                     IFKEPTWNLREQRQQLGV"
     gene            1345..2358
                     /locus_tag="VM910_32255"
     CDS             1345..2358
                     /locus_tag="VM910_32255"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HVI67215.1"
                     /translation="MPVMNMIQALNSAMDVMLARDSNVVIFGEDIGYFGGVFRATEGL
                     QKKYGLTRIFDAPIAEGGIMGAAVGMAAYGLRPVVEIQFADYIYPATDQLVSEAARLR
                     YRSGGDFTAPLTVRSPYGGGIFGGQTHSQSPEAIFTHVAGLKTVIPSNPYDAKGLLIA
                     AIEDDDPVIFFEPKRLYNGPFEGYQDKPVVPWSKHPLSEVPADYFTVPLGKAKIVQEG
                     SDLTILAYGTMVHVSTAAARDANVDAEIIDLRTLLPVDIDTIETSVRKTGRCVIVHEA
                     TRTGGYGAELSALVQEKCFYHLEAPITRVTGWDTPYPHVLEWAYFPGPKRVAEALRET
                     VEA"
     gene            2360..4033
                     /locus_tag="VM910_32260"
     CDS             2360..4033
                     /locus_tag="VM910_32260"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HVI67216.1"
                     /translation="MGHYVFKLPDVGEGTAEAELVAWHIKPGDLVEEDQPVVDVMTDK
                     ATVEIPAPATGKVVSVKGTPGEMLAVGSAILVLEVDGPGNAGTEDVELSATPPPAIPE
                     QKVTNAAPAAPAVINDVSLSAAGGNYVFKLPDVGEGTAEAELVAWHIKPGDMITEDQH
                     ICDVMTDKATVEIPSPTAGKVISTKGTAGQMLAVGSEILVLQVEGPGNMGATPQQQAP
                     SPPKPASPPAKPPPAKAASPAVKPAPSATERRPAAQVASKPGFATRTPGEKPIASPAV
                     RRRAWEAGVELQFVPGTGPGGRISQSDLDSYIESGGSIGQVPATGLRKKVGTEDIKVI
                     GLRRTIAMRMQDAKRRIPHFSYIEEVDVTDLEELRAKFNARWGKTRGKLTLLPFLAKA
                     MINALQDYPQINARFHDDDGFVRRYEGVHLGIATQTPTGLVVPVIQHAEALTLWECAT
                     EIARLAAAAREGKATRDELSGSTISITSLGVLGGVATTPVINYPEVAIVGVNKILERP
                     MVQDGKIVIRKMMNLSSSFDHRVVDGWHAAEFVQQIRDQLETPAMLFVD"
     gene            4037..5449
                     /gene="lpdA"
                     /locus_tag="VM910_32265"
     CDS             4037..5449
                     /gene="lpdA"
                     /locus_tag="VM910_32265"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HVI67217.1"
                     /translation="MTNEIRTKVLVVGSGPGGYVAAIRAGQLGLDTTIVENERLGGTC
                     LIRGCIPSKALIHVAGKFEAMQHATNGGSLGIRLREAPILDLPKTIAWKETIVDKLNS
                     GVGALLKRVKVKVVQGWATFSDAKTCVVNGSGDPVTITAEHVILANGSEPVELPTLPF
                     GGMVLSSTEALSLQEIPEQFVVVGAGYIGLELGIAFRKMGSNVAVVEALDRILPLYDE
                     QLTDPVRKWLARAGVTVHLGAKAKGLTGDTNRRSLQVELKDGQKLDIPADKILVTVGR
                     RPKTKGWGLEKMAVDMVGPFVKVDDQCKTSVKNVWAIGDLVGEPMLAHKASAQAEMVA
                     EIIAGKPRRFRPATIPAVCFTEPEIVTAGMLPAEAKAAGNDVITGLFPLSANGRALSM
                     EGGSEGGFIRIVAQRDNHRVLGAQAVGTHVSELSGEFVHAIEMGAVLEDIAGTIHVHP
                     TLTEAFHESALRALGHAIHI"
     gene            5462..>5581
                     /locus_tag="VM910_32270"
     CDS             5462..>5581
                     /locus_tag="VM910_32270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HVI67218.1"
                     /translation="MQQITTIEELRVIAKKRVPRMFYDYVDSGAWTGSTYEANE"
BASE COUNT         1145 a         1678 c         1768 g          990 t
ORIGIN      
        1 acgggaggac gacgaatgat cggcgccgcc gacccgccgc atcgtatgat gctctccgcg
       61 catgagatct gatctccaaa tcgccaagcg ctctgattga gggagcaaat gacaaattcg
      121 gctcggctaa gtctgcatat cccggagcca aaagttcgcc cgggcgacca gccggacttc
      181 agtgatctga agatcccggc ggccgggtcg gtgccccgac ccgatatcgc agttcgggct
      241 aaagagctgg gcgcttatgc ggatactttg atccgcgtgc tcgacgacaa gggcaaggct
      301 gtcggtccgt gggatcccaa gctcgatccc gaaacgctgc ggcgcggtct gcgcgcgatg
      361 atgcttacgc gcgcttacga cgaccggatg ttccgggtcc agcggcaggg caagacgtcg
      421 ttctacatga aatgcacggg tgaggaagcc gtcgctgtgg ggcaggcgct tgcgcttgaa
      481 aacaccgaca tgtgctttcc gacttatcgt cagcagggcc tgctgatcac ccgcaagtgg
      541 tcgatggtcg acatgatgtg ccaggtcttt tcgaactcac gggaccggct gaagggtcga
      601 cagatgccgg tcttctattc gagcaagcag gccgggttct tcagcatctc cggcaatctc
      661 ggcacgcaat actgccaggc ggtcggctgg gcgatggcgt cagcgctgaa gggcgatacg
      721 cgcatcgcgg ccgggtggat aggcgacggc gctacagccg aaggcgactt ccattacgcc
      781 ctgacctttg cgtcggtgta tcgggtcccg gtcatcctga acatcgtcaa taatcagtgg
      841 gcgatctcca cgtttcaggg ggtcgcgggc ggcgagcagg ctacatttgc ggcgcgtgca
      901 ctgggctacg gacttccggg cttgcgagtg gacggcaatg atctgcttgc cgtgtacgcg
      961 gcgaccaaat gggccgcgga tcgcgcccgc gccaatcatg gcgcgacagt tatagagctc
     1021 tacacgtata gggcttccgc ccattcgacg agcgacgatc cctcgaaata tcggccggcc
     1081 aaggaagagg agaaatggcc tctcggcgat ccgatcgagc ggctcaagca acatctgatc
     1141 gtccgcggcg agtggagtgc ggaccgccac agcgcccagg agaaagaagt cgtggaggaa
     1201 gtgacggcgg cggcgcggga ggccgaaagc tacggcacgc tcgggaacgg gcccactcac
     1261 agcgccagca caatgttcga agatattttc aaggagccga cctggaatct ccgcgaacaa
     1321 cgtcagcagc taggggtttg accgatgccc gtcatgaaca tgatccaggc cctgaattcg
     1381 gccatggacg tcatgctggc gcgtgattcc aacgtcgtca tcttcggcga agatatcgga
     1441 tattttggcg gcgtgtttcg cgcaaccgaa ggtctgcaaa agaaatatgg tcttacccgc
     1501 atcttcgacg cgccgatcgc cgagggcggc atcatgggtg cggcggtcgg catggccgca
     1561 tacggcttgc gacctgtcgt cgagatccag ttcgccgatt acatctatcc ggcgaccgac
     1621 caactcgtat ctgaagccgc gcgccttcgc tatcgttcgg gcggtgactt taccgcgccg
     1681 ctgaccgtgc gctcgcctta tggcggcggc attttcggcg ggcagacgca tagccagagc
     1741 cccgaagcga tctttacgca tgtcgcggga ttgaagacgg tgattccatc caatccgtac
     1801 gatgcaaaag ggctgctgat cgcggcgatc gaggacgacg accccgtcat cttcttcgag
     1861 ccgaagcgcc tctataacgg acccttcgag ggttatcagg ataaacccgt cgttccctgg
     1921 tcgaagcatc ctctgagcga agttccggcg gactacttca ccgtgccgct tggcaaggcg
     1981 aaaatcgtcc aggagggcag tgacctcacc attctcgcct acggcacgat ggtccatgtg
     2041 tcgaccgcgg ccgcgcgcga cgccaatgtg gatgcggaga tcatcgacct gcgcacgctg
     2101 ctgccggtcg atatcgacac gatcgaaaca tcggtaagaa agaccgggcg ctgcgtcatc
     2161 gtgcacgaag cgacacgcac cggtggatac ggcgcggagc tttccgcgct ggtgcaggag
     2221 aagtgttttt atcatctcga agcgccgatc acgcgcgtca ccggttggga cacgccttat
     2281 ccccacgtcc tggaatgggc ctatttcccg ggaccgaagc gggtagccga agcgctgcgc
     2341 gagaccgtgg aggcctgaga tggggcacta cgtcttcaag ctgccagatg tcggggaagg
     2401 caccgccgaa gccgaactcg tcgcgtggca catcaaaccg ggcgacttgg ttgaggagga
     2461 tcagcccgtc gtggacgtca tgacggacaa ggcgacggtc gaaatccctg cgccggcgac
     2521 cggaaaagtc gtctccgtca agggcacgcc aggcgaaatg ctggctgtcg gctcggcgat
     2581 cctcgtgctc gaggtcgatg gtcccggcaa tgccggaacc gaagacgttg aactgtcggc
     2641 tacgccgccc ccggcgatac cggagcagaa ggtcacgaat gccgcgcccg ccgcgccggc
     2701 cgtaatcaac gacgtgtcgc tgagcgccgc cggcggcaat tacgttttca aactgcccga
     2761 tgttggtgag ggaacggcgg aagccgaact cgtcgcatgg cacatcaagc cgggcgacat
     2821 gatcaccgaa gatcagcaca tttgcgacgt gatgacggac aaggccacgg tcgagattcc
     2881 gtcgcccacg gccggcaagg tcatctcgac caagggtacg gccgggcaaa tgctcgcggt
     2941 cggctcggaa atccttgtcc ttcaggtgga gggtcccggg aacatgggcg cgacgccgca
     3001 acagcaggca ccgtcgccgc ccaaaccggc gtctccaccg gcaaaaccac cgcctgcgaa
     3061 agccgcaagc ccggccgtaa agcctgcgcc gtccgcaaca gagcgcagac ccgccgcgca
     3121 ggttgcgtca aagccggggt ttgcaacgcg aactccgggc gagaagccca tcgcctcgcc
     3181 ggccgttcgg cgacgcgcct gggaggccgg cgtcgaactt cagttcgtgc caggcaccgg
     3241 cccgggcggc cggataagcc aatccgacct cgactcatac atcgagtccg gcggcagcat
     3301 tgggcaagtc ccagccaccg gccttcggaa gaaggtcggg acagaggaca tcaaggtcat
     3361 tggccttcgc cggactatcg ccatgcgcat gcaggacgcg aagcgccgca tcccgcattt
     3421 ctcctatatc gaagaggtcg atgtgaccga cctcgaggag ttgcgcgcga aattcaatgc
     3481 gcgatggggc aagacccggg gtaaattgac cctgctgccg ttccttgcga aggcgatgat
     3541 caatgccctg caggattacc cgcagataaa cgctcgcttc catgacgacg acggtttcgt
     3601 gcgccgctat gagggcgtgc atctcggaat cgccacgcag acaccgacgg gcctcgtcgt
     3661 gcccgtcatc cagcatgccg aggcgctgac cttatgggaa tgcgcgacgg agatcgcacg
     3721 acttgcggcg gcggcgcgcg aaggcaaagc gacgcgcgat gaactgtcgg gctcgacgat
     3781 cagcatcaca agtctcggcg ttctgggtgg tgtcgcgacg acgccggtga tcaactatcc
     3841 cgaagtcgca atcgtcggcg tcaacaagat tttggaacgc ccgatggtgc aggacggcaa
     3901 gatcgtgatc cgcaagatga tgaacctgtc atcgtctttc gaccatcgtg tcgtggacgg
     3961 ctggcacgcc gccgagttcg tgcagcagat ccgcgatcag ctcgaaacac cggcgatgct
     4021 tttcgtggac tgaaccatga ccaacgaaat caggacgaaa gttcttgtcg tcggcagcgg
     4081 gccaggcggc tatgtggcgg cgatccgtgc gggacagctt ggcctcgaca cgacgattgt
     4141 cgagaatgag cggctcggcg gcacctgcct catccgcggc tgtatcccct cgaaagcgct
     4201 gattcacgtt gcaggcaaat tcgaggcgat gcagcacgcg acgaatggcg gttcgcttgg
     4261 tatccgcctt cgcgaggcgc cgatcctcga tctacccaag accatcgcgt ggaaggagac
     4321 gatcgtcgac aagctcaaca gcggcgtcgg cgcgcttctc aagcgcgtca aggtgaaggt
     4381 cgtacaggga tgggctacgt tctcggatgc aaagacctgt gtcgtcaacg gtagcggaga
     4441 tccggtgacg attaccgccg aacatgtgat cctggcaaac ggctccgagc cggtcgagtt
     4501 gccgacactg ccgttcggcg gcatggtgct gtcttccacc gaagcgctgt cgcttcagga
     4561 gattcccgaa cagttcgtcg tagtcggggc cggctatatt ggtctcgaac tcggaatcgc
     4621 tttccgcaag atgggctcga acgtcgcggt cgtcgaagcg ctggaccgca tcctgccgct
     4681 gtatgacgag cagctcaccg atcccgttcg gaaatggctc gcccgtgccg gcgtcactgt
     4741 ccatctcgga gcgaaagcga aaggactcac cggcgatacc aatcgcagga gtttgcaggt
     4801 cgagttgaag gacggtcaaa aactcgacat tccggccgac aagatcctcg tcaccgttgg
     4861 ccgccggccg aagacgaaag gttggggcct tgaaaaaatg gccgtggaca tggtcggacc
     4921 cttcgtcaag gtagacgatc agtgcaaaac ttcggtgaag aacgtctggg ctatcggcga
     4981 tctcgtcgga gagccgatgc tcgcgcacaa agcgtcggcg caggcggaaa tggttgccga
     5041 aatcatcgcc ggcaagccac ggcgatttcg gccggcaact atccccgccg tctgtttcac
     5101 cgaacccgag atcgtcacgg ccggcatgct gccggccgaa gccaaggccg ccggcaatga
     5161 tgtaatcacc gggcttttcc ccctgtcagc aaacggcagg gcactgtcga tggaaggcgg
     5221 cagcgaagga gggttcatcc ggatagtcgc gcaacgcgac aatcatcgcg tcctcggcgc
     5281 gcaggcggtc ggcacacatg tgtccgaact ctccggcgaa ttcgtccatg cgatcgagat
     5341 gggcgcggtg ctcgaggata ttgccggcac aattcacgtg cacccgacgc tgactgaagc
     5401 cttccacgaa tcggctttgc gggctttggg ccatgcgatc catatctgac gcgacctgac
     5461 gatgcagcaa atcaccacca tcgaggaact gcgggtcatc gcgaagaaac gcgttccgag
     5521 gatgttctac gattatgtcg attcgggcgc gtggactggc agcacctacg aggcgaacga
     5581 g
//
LOCUS       DATLJT010001406         1579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3335923,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001406 DATLJT010000000
VERSION     DATLJT010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335923"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VM910_32275"
     CDS             complement(<1..272)
                     /locus_tag="VM910_32275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI67219.1"
                     /translation="MNRSSVDKTVAEPGPRPEERAGRPILDVTGLRTYFFTRLGIIKA
                     VDDLSFTLRRRETMAIVGESGCGKSMTALSVMRLIPDPPGRIVAGA"
     gene            complement(274..900)
                     /locus_tag="VM910_32280"
     CDS             complement(274..900)
                     /locus_tag="VM910_32280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67220.1"
                     /translation="MIYGARISLAVGIGATALGSGFGIVFGLTSGYLGGWVDLIVQRI
                     MDILQALPLLVLALVMAAALGPSLQNTIIAISIPLIPYVARVIRSNTLALRELPFVEA
                     AKAVGMSELRIAARHILPNTLAPLIVLATAQLGSTILTEAALSFLGLGVPEPYPSWGR
                     MLSQSAAEYVRTAPWLVIFPGIAISLAVFGTNLLGDALRDILDPRQRS"
BASE COUNT          346 a          475 c          494 g          264 t
ORIGIN      
        1 atcgcgccgg caacgatccg gccgggcgga tcgggaatga gccgcatcac ggagagcgcc
       61 gtcatactct tgccgcagcc ggattcaccg acgatcgcca tcgtctcgcg ccgacgcaga
      121 gtaaacgaga ggtcgtcgac cgctttgatg atcccgagcc gggtaaagaa gtacgtccgc
      181 agcccggtta catcgagaat gggccgtccc gctctttcct cggggcgcgg tcccggctcg
      241 gccactgttt tgtcgacgct cgaccggttc atttcaactg cgctgccttg gatcgagaat
      301 gtcgcgcagt gcatcgccga gaagattggt gccgaacacc gcgaggctga tggcgatgcc
      361 ggggaagatg acgagccacg gcgccgtgcg gacatattcg gcggccgatt gcgacagcat
      421 gcggccccag gatggatacg gctcggggac accgagaccg aggaaggaca gtgccgcttc
      481 ggtgagtatc gtcgagccga gctgggcggt tgcgagcacg atcagtggcg caagggtgtt
      541 gggcaggatg tggcgggccg caatgcgcag ctcgctcatg cccaccgcct tcgcggcctc
      601 gacgaagggt agctcgcgca acgccagcgt attggagcgg atcacgcgcg ccacataggg
      661 gatcagcggg atcgagatgg caatgatggt gttttgcagc gacgggccaa gcgccgccgc
      721 catcaccagc gcaagcacca acagcggcag agcctgcagg atatccatga tgcgttggac
      781 gatgagatcg acccagccac cgaggtaacc ggaggtgagg ccgaagacga tgccgaaccc
      841 gctccccagc gccgtcgcac cgatcccgac cgcgagcgaa atgcgcgcgc cgtagatgat
      901 ccgcgcgtaa acgtcgcaag gtttttcccg tcaataaaag ggcggccctg gtggtggtgg
      961 aacatcaaca gagcggtcgc ccgaacgtag atggtcgtcc cttccgcacc aggacgggtg
     1021 caggggaatc tcgacccgag ctcgatatcc ccaagccgtt caacccgcca aagcctcgcg
     1081 aagatgagcc agggaagatt tttcaaggaa cagggaattt tcacgccaga tgcttcgatt
     1141 ttgaattttg agcagccaca ggtggaggga gcgccccggg tcgcaagcca gcgcaggttc
     1201 aattcgcggg ccgcgcgacg ccttcatacg aagagccctc agccattgtg cagaacacaa
     1261 catgaacata tcttgcatga gaaatccgaa tcggtccaac tgccgcggcc tcgacccttg
     1321 atacagatcg cagcgtcgcc cgttttagac cgttctgaac acgcgatttt gggtgccgat
     1381 tatgcgaaaa atgaacgacc ggctttcgcc gacgcacggt attaataagt gccactcaaa
     1441 aacggagtgg cactgatgtc cccgcaagcg tttagtgatc tcgagcctat cagcaaggcc
     1501 gccaagcggc tcagaaagca cccgcgcaca ctgatgcgct ggacggagaa agcccgacgg
     1561 cctgccatac atccggctt
//
LOCUS       DATLJT010001407         3430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_118688,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001407 DATLJT010000000
VERSION     DATLJT010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118688"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..730)
                     /locus_tag="VM910_32285"
     CDS             complement(23..730)
                     /locus_tag="VM910_32285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HVI67221.1"
                     /translation="MIDLYYWTTPNGHKITMFLEEVGLPYKIFPINIGKGEQFKPEFL
                     TVAPNNRIPAMVDHEPKGGGRPISIFESGAMLLYLAEKTGKFIPSDIYGRYDVIQWTF
                     WQMGGLGPMAGQNHHFRNYALDKIPYAIDRYVNETNRLYGVLNKRLSDREYIGGDYSI
                     ADMACYPWVVPYKNQGQEIEQFPHLKRWLETIKARPATERAYAKAKEVNPNFGQPAIR
                     TEEERKILFGQTSAVVR"
     gene            complement(868..1104)
                     /locus_tag="VM910_32290"
     CDS             complement(868..1104)
                     /locus_tag="VM910_32290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67222.1"
                     /translation="MTETADPLVLDFVEWVAREPRLYAEVVGTWKTSCPRLTIWEDAA
                     DKGYVARETVVGTGLVVTVTEDGAKLLRANGRIA"
     gene            complement(1117..1878)
                     /locus_tag="VM910_32295"
     CDS             complement(1117..1878)
                     /locus_tag="VM910_32295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI67223.1"
                     /translation="MSALMIAALGLLMVATAFLSGLFGMAGGLILIGVLLALMPLPTA
                     MVLHAITQMASNGWRAFLWRAHIRWRPVSVYLIGCALALGLWSITRYVPDKPIALLLL
                     GITPFMARMTPKDLKPNPDSVVQGTIYGSICMGLMLMTGVSGPLLDTFFLGGKFDRRA
                     IIATKATCQVASHFTKLIYFGGIIDQAATLDPVLAFVAVGASMLGTTLSRRVLEAMSD
                     AQFRRWANHLITTVASYYILYAGWLFFARTSVAFY"
     gene            1971..2879
                     /locus_tag="VM910_32300"
     CDS             1971..2879
                     /locus_tag="VM910_32300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI67224.1"
                     /translation="MRRLLFLNGIKAFEAAARGGSFAAAGSELNVSAAAISRMVHLLE
                     QRLGVALFERKANKLALTSAGRAYQSGLTPIFDALASLTAQVTAPTSLRVLTIGVGPT
                     FAMRWLIPRLADFRKQEPDIDVRITAGGVLAPFGDDWSCGIKLGDGAWPGLVAEPLFA
                     ADLLPVCAPRLAAALKRPSDLKGPTLLRVAHASEDWPLWLKAAGVTRITARGTEFQYY
                     GQALQAAVDGLGIAMGIRPYIDDDLAAGRLVAPFPLTVPKGTHWYLVYRSFQAEQRDF
                     AAFRRWIVRAAAAPALHKARHRSARL"
     gene            2937..>3430
                     /locus_tag="VM910_32305"
     CDS             2937..>3430
                     /locus_tag="VM910_32305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67225.1"
                     /translation="MTREASTQLPQPIFHEPIFGEGGALPDPTGFSTRHGSDEALYKQ
                     IGDLAKKDIVAIPKSRSPDEALFSLACAYGHHGAQITQKIEKAGRIIFHALGDSGASN
                     SGKYPDELRVADQVTLDCRASAEADRAAFLFHLGDIVYNFGEARYYYDQFYEPFRNYP
                     APIFA"
BASE COUNT          648 a         1083 c         1056 g          643 t
ORIGIN      
        1 tgacgttgcg caagaatacg ccctatcgca ctaccgcgga ggtctgtccg aacaggatct
       61 tgcgctcttc ctcggtgcgg atcgcgggct gcccgaaatt cggattgacc tccttcgcct
      121 tggcgtaggc gcgctcggta gcgggacgcg ccttgatcgt ctcgagccag cgcttgagat
      181 gcggaaactg ctcgatctcc tggccctggt tcttgtaggg aacgacccag ggatagcagg
      241 ccatgtcggc gatggaataa tcgccgccga tatattcgcg atccgacagg cgcttgttga
      301 gcaccccgta aaggcggttg gtctcgttga catagcggtc gatcgcgtag gggatcttgt
      361 ccagcgcgta attgcggaag tgatggtttt gcccggccat cggcccgagc ccgcccatct
      421 gccagaacgt ccactggatc acatcgtagc ggccgtaaat atccgacggg atgaacttgc
      481 cggtcttctc ggccagatag agcaacattg cgccggactc gaagatggag atcggtctgc
      541 cgccgccctt cggctcgtga tcgaccatcg cggggatgcg gttgttcggt gcaaccgtca
      601 gaaactcggg cttgaactgt tcgcccttgc caatgttgat cgggaagatc ttgtagggaa
      661 ggccgacctc ttcgaggaac atcgtgattt tgtggccgtt cggcgtggtc caataataaa
      721 ggtcgatcat ggcggacgtt tcctgtgcga cgtgcgaggg gatatggggt cgcaaaaaga
      781 tgcgattgca tgaattttgc gcggcccgcg ggtcaagtca atggcatcac gcgcgaaagt
      841 gatcgcgccg cgcattgccc gcaacggtca ggcgatgcgg ccgttggcgc gcaacaattt
      901 ggcgccgtcc tcggtgacgg tgacgaccag gcccgtgccc acgaccgtct cgcgcgcgac
      961 atagcccttg tcggcggcgt cctcccagat cgtcagtcgc gggcacgagg tcttccaggt
     1021 tccgaccacc tccgcgtaaa gccgcggctc gcgcgcgacc cattcgacga aatcgagcac
     1081 cagcggatct gcggtttccg tcatcgctgt tctccttcaa tagaaagcga ctgaagttcg
     1141 tgcaaagaac agccaacccg catagaggat gtaatagctc gcaaccgtcg tgatcaggtg
     1201 gttcgcccag cggcgaaact gcgcatcgct catcgcttcc agcacacggc gcgacagcgt
     1261 cgtgccgagc atcgaggcac cgaccgcgac gaacgccagc acaggatcga gcgttgcggc
     1321 ctgatcgatg ataccgccga aataaatcag cttggtgaaa tggcttgcaa cctggcaggt
     1381 tgccttggtc gcgatgatgg cgcggcggtc gaatttgccg ccgaggaaga aggtgtcgag
     1441 cagcggcccg gagacgccgg tcatcagcat caaccccatg cagatcgagc cgtagatcgt
     1501 gccctgcacc acgctgtcgg gattgggctt caagtccttc ggtgtcatcc gcgccatgaa
     1561 cggggtgatc ccgagcagaa gcagcgcgat cggcttgtcc ggcacgtagc gggtgatcga
     1621 ccatagccca agcgccagcg cgcagccgat caggtagacc gaaaccggcc gccagcggat
     1681 atgcgcgcgc cagaggaaag cgcgccagcc gttggaagcc atctgcgtga tcgcgtgcag
     1741 gaccatcgcg gtcggcagcg gcatcagcgc gagcagcacg ccgatcagga tcagaccgcc
     1801 cgccatgccg aacaggccgg acaggaaggc ggtcgccacc atcaacagcc cgagcgcggc
     1861 aatcatcagc gccgacatgc gaaacctccg tcgatagtag tgccctcctt gtgtcgtccg
     1921 cgcaaacgga gttatcttgc tctcagcatc agttttcctg agggtgggat gtgagacgtc
     1981 tcctcttcct caacggcatc aaggcgttcg aggccgcggc gcgcggcggc agcttcgccg
     2041 ccgcgggaag cgaactcaac gtctccgctg ccgcgatcag ccgcatggtg catctgctcg
     2101 agcagcggct tggcgtcgcg ctgttcgagc gcaaggccaa caaactggcg ctgacatcgg
     2161 ccgggcgcgc ctatcagagc gggctgacgc cgatcttcga tgcgctggcg agcctgaccg
     2221 cgcaggtgac ggcgccgaca agcctgcgcg tcttgaccat cggcgtcggc ccgacctttg
     2281 ccatgcgatg gctgatcccg cggcttgccg attttcgcaa gcaagaaccc gacatcgatg
     2341 tgcgcatcac cgcaggcggc gtgttggccc ccttcggcga tgattggagt tgcggcatca
     2401 agctcggcga cggggcctgg ccgggactgg ttgccgaacc gctgttcgcg gccgacctgt
     2461 tgccggtctg tgccccgaga cttgccgccg cgttgaagcg cccgagcgat ctcaaagggc
     2521 caaccctgtt gcgcgtcgcg cacgcatcgg aggactggcc gctctggctc aaggccgcgg
     2581 gcgtcacccg tatcaccgcg cgaggcaccg aatttcaata ttacgggcaa gcgctgcaag
     2641 cggccgttga tggcctgggt atcgcgatgg gcattcgccc ctatatcgac gacgaccttg
     2701 cagcaggtcg cctggtcgca ccatttccgc tgaccgtgcc gaagggcaca cactggtatc
     2761 tggtctatcg cagttttcag gccgagcagc gcgattttgc cgcctttcgc cggtggatcg
     2821 tgcgcgccgc ggcggcgccg gccctgcaca aggctcgcca tagatccgct cgtctttaga
     2881 ttgtaaactt ggtgctgtat atatttctgc atttgaagaa ggccggagcg attgccatga
     2941 cccgcgaagc ctcgacccaa ttgccgcaac cgatcttcca cgaaccgatc ttcggcgagg
     3001 gcggcgcgct tcccgacccg accggtttct ccacccgtca cggcagcgac gaagcgctct
     3061 acaaacagat cggcgatctc gcgaaaaagg atatcgtcgc gatcccgaag tcgcgatcgc
     3121 ccgacgaagc actgttcagc ctggcttgcg cctatggcca tcatggcgca cagatcacgc
     3181 agaagatcga aaaggcgggc cggatcatct tccacgcact cggcgattcc ggcgcctcca
     3241 attcgggaaa ataccccgat gaattgcgcg tcgccgacca ggtgacgctc gactgccgcg
     3301 cgtcggcgga agccgatcgc gccgctttcc tgtttcacct cggcgacatc gtctacaatt
     3361 tcggcgaggc gcgctattac tacgaccagt tctacgaacc cttccgcaac tatcccgcac
     3421 cgatcttcgc
//
LOCUS       DATLJT010001408        23410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2877570,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001408 DATLJT010000000
VERSION     DATLJT010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 23410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23410
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877570"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VM910_32310"
     CDS             <1..723
                     /locus_tag="VM910_32310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67226.1"
                     /translation="GELAGRADGRCWAEHTTEHDDLRRVAAIEFQPGLDCYKALCDAV
                     DPDDEFSEMEFKRYLFGDESAVLTNEYLAAFIEGHDNSSWKGRPRSNCAAGTAMPPNF
                     VLLDATLPDVTRLVAAMVQARDAVERQSWWHPQEPTSDEWWADVLADPRARKRERRTD
                     LAVKQAFYRLADEKREIILVACSKCDWRAAFSRDDLIATHGADYAMPNLLNRLAAPTC
                     SRLGSHWDRCGVHYVEPIEGAR"
     gene            986..1384
                     /locus_tag="VM910_32315"
     CDS             986..1384
                     /locus_tag="VM910_32315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI67227.1"
                     /translation="MKATRSVPVVAKLVVVIDDDPLILEATEGLLRSWGCCVVTAQSC
                     DEALVRLAETDAGRRPDVIVCDYQLSRGMTGVDAIERLRSAFPIPALPPIPALLISGD
                     ARCEVGSRACRLLYKPVNPERFHAALVDAS"
     gene            1629..1856
                     /locus_tag="VM910_32320"
     CDS             1629..1856
                     /locus_tag="VM910_32320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67228.1"
                     /translation="MTVTIGRRELLAALGGAAAAWPPAARAQQQAMPVIGFGDALPRN
                     SFEGQVDLCIRAGSQFLNLQVERARSLPQVA"
     gene            complement(1940..3058)
                     /locus_tag="VM910_32325"
     CDS             complement(1940..3058)
                     /locus_tag="VM910_32325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67229.1"
                     /translation="MSVFRLAMYPASEGDTLMLTWGEAARPRHALVDLGRTKNYRALK
                     PLLKEIANVDLFIITHIDADHIEGAVPLFKEAPPPFAARHVWFNGYAQLETANERLPP
                     ATRVTLGAGQAEKVTAGIVKSGWRWNDQFASGVVSINSDEAKQPIDFDDGLSVTLLAP
                     TDRKLADLMPVWADELKAAGLRTTDPDEVAEALAEGRVRLGGINVEDLAKARFKEDAT
                     KPNGASIVFIAEYRGKRVLMGADAHPGVMEASLRALGASESNPYRLDCLKVSHHGSKA
                     NTSPGLLKVLDCTCFAFSTDGSHHGHPDPETIARILRADSVRKKTLIFNFRQDSTDVW
                     DDDDLMARWNYECFFPDDRRDGVEIDLYPSLRRRSARS"
     gene            complement(3061..5475)
                     /locus_tag="VM910_32330"
     CDS             complement(3061..5475)
                     /locus_tag="VM910_32330"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI67230.1"
                     /translation="MPSPEKILKADNTGRKVGLVYNDDTLSAGPAAHVLVVGVAAYQS
                     PKYKKVLQTATISARAVADWFVDGAKARFANPACGLGSVALLLSETAGVANALYAGGE
                     IPRATFANAKAALRDWVERINTHKDNLAILYVASHGESFLNRTAFLLEDYGIDSLDAT
                     AGMVEIEQLVGALENAIPVAQLLLFDCCRDATSAELPWNEAIGNKLIALKRRPDDHGE
                     PRKQWVICSTSLGRYAGGLATGATLFNMALIEALNGVASDTSTEGWPVRPGLLVDKID
                     RILALHRLPDERAQTPAGRMAGSFDITFPGELKEVPIYITLNDPAEWPGSTINVRING
                     IPAEPIKGAEGQSPFHVSRVPELAVVEVEAARQNTNLGRAKAKAHPPAMFLAVEKNPA
                     AFSAEVGRLPRSRGLATRAKLVIDVQSQTLIRKGAVASIMRRDQPRASAREVTVDIGG
                     ETPVDFAPGDLTIALRTPDGRTQTRDVTLANYQIVRVQFATQQSPQPWLSAAAIAGAI
                     REPSAEFAATGAASGRNLAVDVVGGINMDLTVSSLADVRMRVMAGPDDGRFARYDVQD
                     ERESRFVREMSPSAPPLFARVTMGDRVEFAVIPSLGGAGQYTVGGWTPFLLVDRRAAL
                     NEPLTAAIVQDRKWSGLLGFLASREIAAGAKLLDSGLSQAAVAAMAEKVSNPLAAVAG
                     ALIAVAASNPDIEKTWDPWLENIDNWFMDIPDGPIVLGRRLLMRARTEKQVAEARRWF
                     IMGFQRGVPFYSLSVDWLAHGLESIPGDDEDRDLARMQRAARHLSGRIDPTHTFSVIR
                     ASDGAA"
     gene            complement(5724..8930)
                     /locus_tag="VM910_32335"
     CDS             complement(5724..8930)
                     /locus_tag="VM910_32335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI67231.1"
                     /translation="MNRPAVAACERPDLVLQTGHTGGVAAVAFSPEDRYLASSSRDNT
                     VKIWDLNKGFELRTLRGHTDSVNAVSFSPDGELLASGSADNTVKLWDVATGRQLFSMP
                     TGNHIMGMVDVAFRSDGKTLISLNQDRTVTRWDVASGRQIGSFSAGGLTGTAWFTRIT
                     RDAHWVISDVRDKNTQAQFIELWDATTGKRVFSCPAGAGDYNNPTLSPDGRWMASNDQ
                     HAAVRMWDVAKGVMIYSLYGDPRKQSNMALGFAFSPDGKILAVGNSERETLQFLEVDT
                     GRMIRSVDGTFTENLRFNSDGSRIAAIRGSEITILNPATGETLQFLRGNTRAISSLAI
                     SRDGRLLAAGGEDNQTRVWDLVMGREIRRVGGPLTRYDLGATAVAFSPDGLWVATNLN
                     EANDEGFLVNRAGIWEVSTGRVVHKLEGFGNGVTSIAFSPDGSLIAAGDYIGRAQIWE
                     THSWNVLGSLKGGSIREGQLWPASSLAFSPDGRWLALSAERGVALWNATTRQWSRSIE
                     EAGYVQALAFSPDSKLLATVNLHSHHIEVWDLEAATCVLSIPEQPAEIGSLKFSADGS
                     RLAAPGLDHTIHAWDAVSGRHLTTLSGHSGEVKAVAFTQDGRWLVSASIDGSVRLWDL
                     SAGSATVLLATISGSDDWVTVTTDGLFDGSTDGTQKLVAWRIGNTLYPADRFYASNYT
                     PRLLGRVLVGEKLASELKLDALRLPPVVRISNPPVGGKSKSGQAQVVVEATDQGGGVA
                     EVRLFQNGKLIAKAPGDSGGSYRFDVDLVAGENVLKASALSQERVEANDDQVRVTNDA
                     DTADPVPARSALHLLVTGVSQYEDSALDLDYARPDAESIAEFFRQNKLFSSVHSYKLY
                     DQGASKIAIQEALEKLVERAQANDVVIIYFAGHGVGVSEQFYFLPHDMRLEDDDVAAI
                     RKYGIPASALADALMRVKALKQVLILDTCESEGALPLLAKSMMYRSRGVSVAEEKAVK
                     MLARAYGVYLIAASARQQDAYEVPELGHGILTYALLRGLGENGEPQAPMLTPGIVTVG
                     SLIEYVYAQVPELTEKYRHQKQYVVTSTTGTNFPLWSN"
     gene            complement(10111..10521)
                     /locus_tag="VM910_32340"
     CDS             complement(10111..10521)
                     /locus_tag="VM910_32340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67232.1"
                     /translation="MPANTLLPIELDPNALAHLDFAEFCDKLIEKLSDIKTQRFAWYD
                     NKRDEQSKWVNGSRRVLATLGSVAILLTAMGTTLRIVDVPHKYEAAGLGCCVGAILGR
                     SGCRAIVAISVAEHLLRVTIQMWKNKTFLVASSS"
     gene            complement(10588..12078)
                     /locus_tag="VM910_32345"
     CDS             complement(10588..12078)
                     /locus_tag="VM910_32345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67233.1"
                     /translation="MQAPASGVKVRMYRQGHGDCFLLAFPRPNGQPAYVLIDCGYKPG
                     SNTRFGLADISNIVANIRESTGGHLDLVIITHEHQDHVNGLPNNFKNFEIGEAWFAWT
                     EDPDDEVANELRKRHHDQLLGLVAARNRLAASSIQAAAWLDSLLQLELGIDNPTEFAA
                     AAKKDPADSINKQGMSLIKKKAGKKNTKYVRPHQEIMSIPGVKGVRVFAFGPPRDPDL
                     ISDEDPEGNEAFPGHGISAFASFYAAARAADGEPEQNTLTFPARLSVPLKEAFRHPEC
                     GKFFSDHYGHDKTPRAIGDESADDASWRRIDQDWLYSAEDLALVLNRGINNNSLVLAF
                     ELLNTKKVLLFIGDTQRGNWVSWTQGSWKDGQESISVKDLMSRTVLYKVGHHGSHNAT
                     LHGTEADAYPNLSWMGHGKYGSEFAAMITAVNKWALAVKPKPWVHPLPSIKKALMEKC
                     GGRVFQTDTPEVTRPEGVSAAEWQEFERRTNIDPAGLYFEYEVHDA"
     gene            complement(12285..14363)
                     /locus_tag="VM910_32350"
     CDS             complement(12285..14363)
                     /locus_tag="VM910_32350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67234.1"
                     /translation="MMLLSILDKPPVRRLRVYALDPSLAQKLQTAPLQEIVLPIRWEQ
                     NPKTGRLDVGPIGEYIEVVDVDPASGVVYPPVDLDDPNLLAQDGLPPSEGYPQFHQQM
                     VYAVAMATIERFEQALGRVALWAPRQVTPRDNPEGQWKHEFVRRLRMYPHALRDQNAY
                     YSPDKKAILFGYFPVRTNDARNTPGTTVFACLSHDVIAHEVTHALLDGVHPRFNEQSN
                     PDVPAFHEAFADIVALFQRFSYTDVVRDEIARTRGRLENENMLAKLAQQFGSATGRGS
                     ALRDALGMEVDGKWQRRPPDTTILEKTMEPHERGAILVAAVFGAFLLVYGRRTEDLYR
                     IATQGSGVLPEGEIHPDMARRLAEEAAACAGNILRMCIRAIDYCPPVNITFGEYLRAV
                     MTADINIDPEDELRYRVAFVESFRQWGIYPRGTRSMSVEALTWPTGAEVIAESAQQLK
                     LRHGKTARISTKKAVAAALKQSQEDIKVFFDQAQVVNGSRAQSMRWDLDSDRLEIWKH
                     MQENRRAVWGWLHRSRGRKYCKAFGLVIGKDAPATVYRKKTGEPTVEVHSVRTALRRD
                     AHGSIVTDLVVEITQRRRGYFKKEDQEKADKRSTPFSAKEKGDFKYRAGCTIIIDTKQ
                     NVFRHIIRTPGPIDDDIELERIRQFPTGDAGPSANAFAGMRAVSLRERAYRGLNEPFA
                     LLIGIQGSES"
     gene            complement(14399..14716)
                     /locus_tag="VM910_32355"
     CDS             complement(14399..14716)
                     /locus_tag="VM910_32355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67235.1"
                     /translation="MTVTIGRRELLAALGGAVAWPLAARAQQREKMRHIGVLVPAASN
                     DPAFQARVGAFLQGLAVLDWSIGRNVRVDARWAIDAAEIRRQAAGLVALAHDWALGSR
                     REA"
     gene            complement(15006..16364)
                     /locus_tag="VM910_32360"
     CDS             complement(15006..16364)
                     /locus_tag="VM910_32360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67236.1"
                     /translation="MARASHTKSTRTPHRPTRAPANPGWRAKVRMYRQGLRDCFLVSL
                     PRAGSRPYSIMIDCGVILGTPADGRRPQMKEVVDNIVATTQGEIDLLVVTHEHWDHVS
                     GFVQAKESFRPLKVHALWLAWTEDPEHELARKLRRERDAALSALRLGLSQLQLAGAKG
                     AVEIAGVLEFLGAAKGTSTSDALAHARSLVRKPRYCLPAEAPTELPDVGARIYVLGPP
                     PCEKRLRKIRPSMREKETYGLGLEALPLFMAGIGAALEGGDAGRPFDRKFEIPKSTAA
                     RLPFFRDHYCNDDRRRIEGDWLMGIPDIALQLDSLTNNTSLVLAIELADGDVLLFAAD
                     AQVGNWLSWQDLAWPVGGRTVTGPDLLNRTILYKVGHHGSHNATLRHYGLELMKRLRV
                     AMLPVDEEMAKRKGWTQMPLPELVTALEQRASGGVLRVDRPSPVKKEQVIEDRLFFEV
                     VF"
     gene            complement(16374..18347)
                     /locus_tag="VM910_32365"
     CDS             complement(16374..18347)
                     /locus_tag="VM910_32365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67237.1"
                     /translation="MSRKQTKNRTRITRLRSKATKARPHIDRLGAANADLKKKPAEAL
                     ELDINVNVPPPRCRPLRVFAYDPSLAMRMDTVDINEATLLVPWEEIQPGPVGEYIEVV
                     DYDPASNCFYAPVDLNHPHILTQSGLTPSEALPQFHQPMVYAVAMKTIEYFERALGRV
                     ALWRHRVQVRDGGVRWEYVQRLRIYPHALRIRNAYFSPARMSLLLGYFKASDTMPGTV
                     FAATSHDIIAHETAHALLDGLRAQFFEPTNPDIRAFHEAFADIVALFQHFTLPEALRH
                     QIAATRGNLEQQNLLGLLAIQFGAATGRGALRDYIGEYVDVNDADGQRLVWRRRKPSQ
                     TDYRDATEPHDRGAVLVAAVFDAFLQIYKRRTRDLLRLATGGTEILAAGAIPADLVNR
                     LAQEASKVARHILDICIRALDYCPPVDMTFGEYLRALITADTDLVPVDEHRYRTAFIA
                     AFRDRGIYPGDAKHMSPDSLVWEPPTAPLRDIRRVLERMSIEWDLNIDRLNAELLSQR
                     NAKLFWQWLHDDREVSDKEVAALGLVRLDRPGPYRLGSHLGELSRIAVQSVRPARRAD
                     PGGVIHWDVVIELTQTFTPAEQPRVRFRGGCTLIINFGSALVRYVVRKRVGNALRFAE
                     QMAFGGAGEVANNYFTADAIGSEPFAALHGMYG"
     gene            complement(18380..19921)
                     /locus_tag="VM910_32370"
     CDS             complement(18380..19921)
                     /locus_tag="VM910_32370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67238.1"
                     /translation="MAKQTKRKPAAAAVSPLKPPPGGAVVRMYRIGHGDCFLIAFAGK
                     KPDKPAYVLIDCGYKPGSPAELANPTKVKDIGADIIATTGGFVDVAIVTHEHQDHVNG
                     LTAKNFPELKVGEAWFAWTEDGHDAIANKLRKKFNDRLLGLIDASSQLHRLALTEAAD
                     KVADFLEFELGEDPRGFRGTGLGAAGKDPASSVNKLAMKFLRDCADGDPVYLLPHEKP
                     VPIRGAASARAFALGPPHDIDKIDDLDPEGEESFGDDDHAQAVASGGVGTGGSARKSP
                     FPRRHVIPLEGAFTHPEFGAFFTVHFGNQNVADAADYADIPDNVPWRRIGADCAADAA
                     ALALAMSNATNNSSLVLAFELSKGGKVLLFAGDAQAGNWRSWSEQPFADGNAEVTAKD
                     LLARTVLYKVGHHGSHNATLHGELKSNYANLSWIAQGETRGEFVAMITAVEKWAHHKP
                     KPDWNHPFPAIKEALEEKAGGRVLQTDCDLQDNPPAGAKASEWRAFRKRLTQTPLFFD
                     LMIEG"
     gene            complement(19926..22001)
                     /locus_tag="VM910_32375"
     CDS             complement(19926..22001)
                     /locus_tag="VM910_32375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67239.1"
                     /translation="MTTIVRYPRPNARRLRIYSFDPALAARYDLVGTSSVTIEVPWED
                     NLRPGPIGGYVEVVDVDPAAGAVYGPVDLNTADLLATDGLQPSESDPRFHQQMVYAVT
                     MKTIEQFERALGRKALWSTYRYQTAERKYVENHVPRLRIYPHAMRQQNAYYSPQKKAL
                     LFGYFPASARNGANTAGTTVFTCLSHDIIAHETTHALLDGVHPRFNEPVNEDVLAFHE
                     AFADIVALFQHFTYPGVLRDQIARTRGDLLRESVLGQLAQQFALASGRGDALRSYLGG
                     KDASGKWQPKKPDPFLLAKTSEPHDRGAILVAAVFGAFMKVYIGRTRDLFRIASEGTG
                     IMREGDIHPDLVNRLAEEAQRCADRALQMSIRAIDYCPPVGITFGDYLRAIVTADLDM
                     YPADVENWRLAFIESFREWGISPRGVLSMAEDSLLWPTEEEVIAEQVTASTATPAAQD
                     LVHADLRMATARGSSAVKKMFLDLGAKPKDEYYASKVTPPASIVPGASEKVLTPVSLS
                     LSRLQIWLEGERQAALFHNWLLDEDAEWLANCLGLVVTPSSLPMTIRQRGGRPLVEIH
                     SVRTVMKRGALGWPEKHLVVVVTQTRIGYFDTETQVQKDAIPDAWKDPQPDFKYRAGC
                     TLLIDPERTQIKRVVRTPGTVADEREFDRLRSYFVRGLEPPNAFDGAAARLKPDAEPF
                     ALLHSRFGA"
     gene            22467..22988
                     /locus_tag="VM910_32380"
     CDS             22467..22988
                     /locus_tag="VM910_32380"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67240.1"
                     /translation="MPYRHHDAVPRPRKYSRYGHRDATAILIAYRHGLRASELCELTW
                     NMIELNSGRIHVRRAKNGVDSTHPLTGKEIRALRQLRRENLQSRYVFNTERGGPVTRA
                     WFLKMVRRTGELAKLPFPIHPHMLRHGCGFRLANDGVDTRALQHFLGHRNIMHTVRYT
                     ELRSDRFDGFWKD"
     gene            23018..23167
                     /locus_tag="VM910_32385"
     CDS             23018..23167
                     /locus_tag="VM910_32385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67241.1"
                     /translation="MLIVTQLAAVGHRGPHSDRDLVIYSWHAWVVVDGDEIAGFSLCG
                     SGDVR"
     gene            23221..>23410
                     /locus_tag="VM910_32390"
     CDS             23221..>23410
                     /locus_tag="VM910_32390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67242.1"
                     /translation="MKRTSLTHPLQIAVVTTGPKFGRVGITFCPGQYDRHAMSGYQDR
                     DLALDLDTVLDWGAAAVVT"
BASE COUNT         4359 a         7239 c         7027 g         4785 t
ORIGIN      
        1 ggcgagctcg ccggtagggc ggacggccga tgctgggcgg agcacacgac cgagcatgac
       61 gaccttcgca gggtggcggc aatcgaattt cagcccggat tggattgcta taaggctctc
      121 tgcgacgccg tcgaccccga cgacgagttc tcggaaatgg aatttaaaag atatctgttc
      181 ggggacgaga gcgccgtcct cacaaacgaa tatttggcgg cgttcattga agggcacgac
      241 aattcttcct ggaaggggcg cccaagatct aactgcgccg caggcacggc catgccgccg
      301 aacttcgtcc tgctcgatgc gaccttgccc gacgtcacga ggctggtggc cgccatggtg
      361 caagcgcgtg acgcagtcga gcgccagtcc tggtggcatc cgcaagagcc gaccagcgat
      421 gaatggtggg ccgacgtgct ggcagacccg cgcgctcgca agcgagagcg gcgcaccgac
      481 ttagccgtca agcaagcgtt ttatcggctc gccgatgaaa agcgcgagat catcctcgtc
      541 gcgtgcagca agtgcgactg gcgggccgcg ttttcgcgcg acgacctgat cgccacgcat
      601 ggtgcggact acgcgatgcc caacctactc aaccgactcg ctgcaccgac ttgcagcagg
      661 ctcggctccc attgggatcg ctgcggcgtc cactatgtcg agcccatcga gggcgcgcgg
      721 tagctaggaa gtgcggctgt gccggtagga cgcccgcagt tcgagcatca cgaaaaccgc
      781 acgccgacgc acggctacgc ggcgacgctc gaggccgcta acgcgtcgcc gtagagaatt
      841 caagtcctgg gacagactat cgtaagtacc ggcgcatata ttgttgaggg tgggacgaaa
      901 gcacctgccg cagatacttc tgatgacttt tttcggctgg agcgcgtcaa tatgattacg
      961 gagttttctt catcaggccc aggtcatgaa agcgacccgc agtgtaccag tcgtcgccaa
     1021 gcttgtcgtc gtaatcgacg acgatcccct gatccttgag gcaactgagg ggctgctgcg
     1081 ctcctggggc tgctgcgtcg tcaccgccca atcctgcgac gaggccttag tacgcctcgc
     1141 tgaaaccgac gcgggtcggc ggcctgacgt gatcgtgtgc gactatcaat tgtcacgagg
     1201 aatgaccgga gtcgacgcca tcgaacggct ccgcagcgcg ttcccgattc cagcccttcc
     1261 cccgattcca gcgctcctca tcagcggcga tgcgaggtgc gaggtcggct cccgagcatg
     1321 ccgccttttg tacaagcccg tgaatccgga gaggtttcac gcggcgctgg tcgacgcctc
     1381 ctagaaatat cgaatcgcga gcaaacgcgg caatcgcgat cagactgcgc actgtgcgtc
     1441 ggacggatca taaaggtggg ccgccgcgcc cgttcggtac gccgtggatg ttgacgctgg
     1501 cctttggcca aagcccacga cagaggtgaa agcaaccagc atcctgccct ccttgatcct
     1561 tgaacggcaa gactgcacgc tcgtggcgtg gtatcgttcc ctttacgcac ctcgcacggg
     1621 aggggcgtat gacagtcacc atcggacggc gggagttgct ggctgcactc ggcggcgcgg
     1681 cggcagcttg gccgcccgcg gcgcgcgcgc agcagcaggc gatgccggtg atcggatttg
     1741 gcgatgcgct gccgcgcaat agtttcgaag gccaggtcga tctctgcatc cgtgctggct
     1801 cgcaattctt gaacttgcaa gtcgagcgcg cgcgcagcct cccgcaggtc gcataactga
     1861 ccttcatagg atgggaagtt cgggtccatc ccgcagtttg gctcccttcg ccgtcgtcgg
     1921 cgagggttcg cgagaccact catgatcgtg cggagcgccg ccggagcgac ggataaaggt
     1981 cgatctccac gccatcccgc ctgtcatccg gaaagaagca ctcatagttc catcgcgcca
     2041 tcagatcgtc gtcatcccat acgtcggtgc tgtcctgccg aaagttgaag attagcgttt
     2101 tcttgcggac ggagtcggcc ctgaggatgc gagcgatcgt ttcggggtcg ggatgaccgt
     2161 ggtgcgagcc gtcggtcgag aaggcgaagc acgtgcagtc caggaccttg agcaggccgg
     2221 gcgaggtgtt ggccttgctc ccatgatgcg acactttcag gcagtcgagc cggtatgggt
     2281 tcgactcgct cgcgcccagc gcccggagcg acgcttccat gacgccggga tgggcgtctg
     2341 ctcccataag cacgcgcttt ccacgatact cggcgatgaa gacgatgctc gccccgttcg
     2401 gcttggtagc gtcctccttg aatctggcct tggcgaggtc ctccacgttg atcccgccga
     2461 gacgcacccg gccctccgcc agggcttccg ccacctcgtc gggatcggtc gtccgtaggc
     2521 cggcggcctt caattcatcg gcccagaccg gcatcaggtc ggcgagcttc cggtccgtgg
     2581 gtgccagcaa ggtcaccgac agtccatcat cgaagtcgat cggctgcttc gcctcgtcgc
     2641 tgttgatgga gaccacgccg ctggcgaatt gatcgttcca tcgccagccg ctcttgacga
     2701 tgccggcagt caccttctcc gcctgtccgg cgccgagcgt cacgcgggta gccggtggca
     2761 ggcgctcgtt ggcggtctcc aactgcgcat agccgttgaa ccagacatgg cgcgccgcga
     2821 acggcggcgg cgcttcctta aagagcggga cggcaccttc gatgtggtcg gcatcgatat
     2881 gggtgatgat gaagagatcg acgttggcaa tctccttcag cagcggcttt agcgcccggt
     2941 agttcttcgt tcgtccgaga tcgacgagag cgtggcgtgg ccgcgccgcc tcgccccagg
     3001 tcagcatcag cgtgtcgccc tccgacgccg gatacatggc gagcctaaag acggacacgc
     3061 tcacgctgct ccgtcgctag cgcggatcac gctgaaggta tgggtcggat ctattcggcc
     3121 ggacaggtgg cgcgcagctc gttgcatacg ggcaaggtca cggtcctcgt cgtcgcctgg
     3181 aatgctctca aggccgtggg cgagccagtc aacggacagc gaataaaagg gaacgccgcg
     3241 ctggaagccc atgataaacc atctgcgcgc ctcggcgacc tgcttttccg tgcgggcccg
     3301 catcagcagc ctgcggccga ggacgatcgg gccgtccggg atatccatga accaattgtc
     3361 gatgttctca agccagggat cccatgtctt ctctatatca ggattactgg ccgcgacggc
     3421 gatcagcgcg ccggcgaccg cggcgagggg attgctgacc ttttcagcca tcgcggcgac
     3481 cgcggcctgg ctcaagccgc tgtcgaggag cttcgcccct gctgcgatct cgcgtgaggc
     3541 caagaagccg agcaggcctg accatttcct gtcctggacg atcgcggcgg tcagaggctc
     3601 gttgagcgct gcccgccggt cgaccaggag gaaaggggtc cagccgccga ctgtgtattg
     3661 accggcgccg ccgaggctcg gtatcaccgc gaattctacc cggtcgccca tggtgacgcg
     3721 agcaaagagg ggtggggccg agggagacat ctcacgaacg aaccgcgact cccgctcgtc
     3781 ctgcacatca taccgcgcga accgcccatc atcggggccc gccatgacac gcattctcac
     3841 gtccgccagc gaactgacag tgaggtccat attgatcccg ccgacaacgt cgactgcgag
     3901 attgcgacca gaagcagcac cggtcgcggc gaattcggcc gagggctccc tgatcgcacc
     3961 agcgatcgcg gctgcgctca gccacggctg cggcgactgc tgcgtggcaa actgcacccg
     4021 gacaatttgg tagttcgcca gcgtgacgtc gcgggtttga gtgcggccgt ccggcgtgcg
     4081 cagcgcgatc gtgagatcgc ccggggcaaa gtcgaccggc gtctcgccgc cgatgtcaac
     4141 cgtgacctcc ctggcgctcg ctcttggctg gtcgcgcctc atgatgctgg cgaccgcgcc
     4201 ctttcggatc agggtctggc tctggacgtc gatgacgagc ttcgcgcgcg ttgcgagacc
     4261 acgcgaccgc gggagtcgtc cgacctcggc ggagaaggcc gcagggtttt tctccacggc
     4321 caagaacatc gcggggggat gcgctttcgc cttggcccgg ccaaggttag tattttggcg
     4381 tgcagcttcc acctcgacca ctgccagctc gggcactctg ctcacatgga atggcgactg
     4441 cccttccgcg cccttgatcg gctcggcagg tatgccatta atcctgacgt tgatcgtact
     4501 gcctggccac tccgccggat cgttcagcgt gatgtagatc ggcacctcct tgagctcgcc
     4561 ggggaacgtg atgtcgaagg agccagccat tcggcccgct ggagtctgag ccctctcatc
     4621 cggcaatcgg tgcagcgcta ggatgcggtc gatcttgtcc acgaggaggc cgggccggac
     4681 cggccagcct tcggtgctcg tgtcgctcgc cacgccattc aacgcctcga tcagggccat
     4741 gttgaacagg gtggccccgg tggcgaggcc gccggcatat ctaccgagcg acgtcgagca
     4801 gatcacccat tgctttcttg gctcgccgtg gtcgtccggc cggcgcttca gggcgatcag
     4861 cttgtttcca atcgcctcgt tccacggcag ctcagccgag gtcgcatcgc ggcagcagtc
     4921 aaacaggagc aattgagcga ccggaatcgc gttctccagc gcgccaacga gctgctcgat
     4981 ctccaccatg cctgcggtgg catcgagcga atctattccg tagtcctcaa gcaggaaggc
     5041 cgtccggttc aggaagcttt ccccatggct tgcgacgtac aggatggcca gattgtcctt
     5101 gtgcgtattg atccgctcca cccaatcccg gagggcagct ttcgcgttgg cgaacgtcgc
     5161 acgcggaatc tcgcccccgg cgtagagcgc gttggcaact cctgccgtct ccgaaagcag
     5221 gagcgcgacg cttcccaatc cgcaagcggg attggcaaag cgcgccttcg ctccatctac
     5281 gaaccaatct gctaccgcgc gcgccgagat cgtggcggtc tgcagcactt tcttgtattt
     5341 tggtgactga taggcggcca cgccgacgac cagcacgtgg gcggctgggc cggccgaaag
     5401 cgtgtcgtcg ttgtagacga gcccgacttt tctccccgta ttgtcggcct tcaaaatttt
     5461 ctcgggcgag ggcatcgcgt gtcctaaacc ctaatcaggc acgtcctttt ggtgtgccgt
     5521 taagacattt gatctcccgg ctgctgattt gcaatggctc gagccgatgg cagacaggac
     5581 ctcgtgttga agcaccagca attgcaccta caacccggaa acccgcagct ccaggatttt
     5641 atgatctgtc tcctcgtcct cgagggtcgt ggagaatggc actgcgcgtc gggacgttct
     5701 tgctgccctc cacctgattg ccctcagttt gaccacaagg ggaaattcgt gccggtcgtg
     5761 gaggtcacca cgtactgctt ctgatgccga tatttttctg tcagctcggg cacctgggca
     5821 tagacgtact cgatgagcga cccgacggtg acaattcccg gcgtcaacat tggcgcctgg
     5881 ggttcaccgt tttctcccaa tcctctaagg agagcgtagg tcagaatacc atgccccaac
     5941 tctggaactt cgtaggcatc ctgctgccga gcggaagctg cgatcaaata gacgccgtac
     6001 gcccgggcga gcatctttac tgccttttct tctgccacgc tgaccccgcg cgaccgatac
     6061 atcattgact tggcgagcag tggcaatgcg ccttccgatt cacaagtatc caggatcaac
     6121 acctgtttca gtgccttgac tctcatcagg gcatcagcaa gggctgaagc aggaatgcca
     6181 tatttgcgaa tggcggcaac atcatcatct tccaggcgca tgtcgtgcgg aagaaagtag
     6241 aattgctcgc tcacgccgac gccatgtccc gcaaaataga tgatgactac gtcgttggcc
     6301 tgcgcccgtt cgacgagctt ctccaacgcc tcctggattg cgatcttgga cgccccctga
     6361 tcgtagagct tgtaggagtg gacgctgctg aacagcttgt tctgtcggaa aaactcggca
     6421 attgattcgg cgtcaggtcg cgcataatcc agatcgagtg cggagtcctc atattggctg
     6481 acgccggtca cgaggagatg caaagctgac ctggcaggga cggggtcggc tgtatcagca
     6541 tcgttggtca cccgtacctg gtcgtcattg gcttccaccc tctcctgact gagcgccgag
     6601 gccttcagga cattttctcc ggcgacgaga tcaacatcga accgatagct gccgccgctg
     6661 tcgcccggcg cttttgcaat cagtttgccg ttctgaaaca gacgcacttc ggcgacgcca
     6721 ccgccttgat ccgtggcctc gaccaccact tgtgcctgcc cactcttcga tttgcctccc
     6781 accggcggat tgctgatacg caccaccggc ggaagtctga gcgcatccaa cttcaattcc
     6841 gacgcgagtt tctctcccac caacacgcgg cccaaaagcc gtggcgtgta attgctcgcg
     6901 tagaaccggt ccgctggata gagagtattt ccgattcgcc aggcgaccag cttttgcgtg
     6961 ccgtccgtcg atccatcgaa cagaccatcc gttgtcacag tgacccaatc gtcactgccg
     7021 ctgatcgtgg caagcaacac agtcgccgat ccggccgaca aatcccagag gcgcacgctc
     7081 ccatcgatgc tcgccgagac cagccaacgg ccgtcctgtg tgaacgccac ggctttgacc
     7141 tctcccgaat gaccagatag cgtcgtcaaa tgcctgccgc tgactgcgtc ccacgcatgt
     7201 attgtgtgat cgagtccagg agcggccagt cggctgccgt cagcactgaa tttcaaactc
     7261 ccgatttcgg ccggttgctc cggtatcgac aacacgcacg ttgcggcttc gaggtcccac
     7321 acttcgatat gatggctgtg cagattcacg gttgccagca gtttgctgtc cggactgaag
     7381 gcgagcgcct ggacgtagcc ggcctcttcg atgctgcgcg accattgacg agttgtggcg
     7441 ttccaaaggg caactccgcg ctcggcgctg agggcgagcc aacgcccgtc cggactgaag
     7501 gcaagagaac tcgcaggcca aagctgacct tctctgatac tgcccccttt gagactgccc
     7561 aagacattcc agctgtgtgt ttcccagatc tgcgccctgc cgatgtagtc tcccgcggcg
     7621 atcagcgagc cgtccgggct gaaagctatc gatgtaacgc cgttaccgaa gccctccaat
     7681 ttgtgcacga ctcgtccggt cgagacttcc cagatgccgg ctctgtttac cagaaaaccc
     7741 tcatcgttgg cttcgttgag atttgtcgcg acccagagtc cgtccgggct gaatgccacg
     7801 gcagttgccc ccaagtcata tctggtcagt ggtccgccga cccgccgtat ctcccgtccc
     7861 atgacgagat cccacaccct ggtctgattg tcttcgccgc ccgccgccag caaccggccg
     7921 tcgcggctga ttgccaggga gctgatagct cgagtgttgc cgcgcaaaaa ctgcagcgtc
     7981 tcgccggtgg ccgggttcaa gattgtgatt tccgagccac ggattgcagc gattctgctg
     8041 ccgtcgctgt tgaaacgcag gttttccgta aacgtaccgt cgaccgaccg gatcatgcgg
     8101 ccggtgtcga cctcaagaaa ctgcaatgtt tcccgctctg aattgccaac ggcaaggatc
     8161 ttgccatcgg ggctgaacgc aaacccgagc gccatgttcg actgttttcg cgggtcacca
     8221 tacagcgaat agatcatcac gcctttcgcg acatcccaca tccgtacggc ggcgtgttgg
     8281 tcgttcgacg ccatccagcg cccgtctggg ctcagcgtcg ggttgttata gtctcccgca
     8341 ccggcgggac agctaaacac tctctttccg gtggtggcgt cccaaagctc gatgaattgc
     8401 gcttgagtgt ttttgtctcg cacatcggag atcacccagt gtgcatcgcg ggtgatgcga
     8461 gtgaaccagg cagtgccggt gaggcctcct gcagagaaac taccgatctg ccgaccgctc
     8521 gcgacgtccc agcgggtcac cgtacgatct tggttcagcg aaatcagcgt cttgccgtcg
     8581 gaacggaacg cgacgtcgac catccccatg atgtggttgc ccgtcggcat cgaaaacaat
     8641 tgtcgccccg tcgcgacgtc ccataacttc actgtattgt cggcgctgcc cgaggcgaga
     8701 agctcaccat cagggctgaa agacacggcg ttcacggaat cggtatggcc gcgcagtgta
     8761 cggagctcga atcccttgtt cagatcccaa atcttgactg tgttgtcgcg gctcgacgat
     8821 gcgaggtatc ggtcctccgg gctgaatgca actgccgcga ctcctccggt atgacccgtc
     8881 tggagcacca ggtccgggcg ctcgcatgca gcaacagccg ggcgattcac gtctggtatt
     8941 cgcggcactg caacctcctg ttggacatca ttgctccccg gcgtgatggg cgcggctgat
     9001 ctggcggcag gttggcaaca gcttcggcga tttcttcaac tttcatgcgg cgcagtatag
     9061 cataggcgca cacgcttggt tgagcgacgg aatagcgatt gggagtccca acggtggtcg
     9121 agcaacccgg cacagccacg tttgaccccg aggagaagat acctggctgg aactatccaa
     9181 catggcggtt cttatattag aggtacttcc gccgtcgcag agcctcgcgg gtaagcatcg
     9241 tagatggcgg tgcacggtga aacaacagca gatgttgtgg gcaatgattg gatcaaaagc
     9301 gtccaacaga tcgtccaaca ttgattgctc acaccggaaa tgcgtaacga tccgattgcc
     9361 gtcagcaagg ctctttatgc gcacctcgag cgaactttgg attcaacacc gtgacgcgca
     9421 acagggaatc tcgaatttaa ttttcgcggg actccccgtg gctacgaggc tcttcgcatc
     9481 cgcccgcgcg caggtgcgat gccaaaaatc aacatggcgc gatcacctgc tgtcggtcgc
     9541 ggtctcggtc gcggccgtcc cggtcaatca cccccgagat cacgaatacg ctccctatag
     9601 acacgctcca gacaaccctg gctgttgccg cactcgttcc gacgcacgat ccacgcccgc
     9661 tccgaagcag tagacacatc actcctccga aacgtttcat tcaactgtct atcgagccta
     9721 cgaaggtcgt cgctgctgca gattgtcctc tccgcctcgt tgagctgcgc ttgactttca
     9781 caccagcgtt gtgcgttgac agacgaggac atcaccagta accctatgaa gccaccaaga
     9841 atcgcgatac ctttaatcat gattggtacc tcctttgtcc cagcgctaag accgtggcat
     9901 tttccgtttg tcgtagttca acattaccag cgcggcagcg catagtccga ccgacgcggg
     9961 gggactatct taggcatagc ttcacctcca tcagtctgga gtcaattcag gcttctatcg
    10021 tggattgtgc catccgtttt aaaatctcag tgtgacatac ttcacacaag ttttcgactc
    10081 tataaaaaag gctggcatgc cgggttttcg tcatgaactt gatgcaacca aaaaggtctt
    10141 gtttttccac atctgaatag ttacgcgcaa caaatgttca gcgacagaaa tggccacaat
    10201 tgctctgcag ccactgcggc cgagaatggc accaacgcaa cagccaaggc ccgcagcctc
    10261 gtatttgtgg ggcacgtcga caattcgaag cgttgttccc atcgcggtga gcaaaatcgc
    10321 gacgcttccc agtgtagcga gcacgcgccg agagccattg acccatttcg actgttcgtc
    10381 tcgcttgttg tcgtaccaag cgaaccgctg agttttaatg tcgctgagct tctcgatcag
    10441 cttgtcgcaa aactccgcaa aatcaaggtg cgcaagcgcg ttgggatcca gctcgatggg
    10501 cagcagagtg ttggcaggca ttagagatca ccctgccggc tcgatatgtg ctatggcgta
    10561 atatttaacg gacgtggaac tgcaagatca tgcatcgtgc acctcgtatt cgaaatacag
    10621 gccggcagga tctatatttg tcctcctctc gaactcctgc cactcagctg ccgatactcc
    10681 ttccggccgc gtgacctcag gcgtatcggt ttggaatacc ctgcctccac acttttccat
    10741 gagagccttt ttgatcgagg gcagcgggtg aacccacggt tttggcttta cggctaacgc
    10801 ccatttgttc accgctgtaa tcattgcagc gaactcgctc ccatatttgc cgtggcccat
    10861 ccagctgagg ttcggataag catcggcctc ggtcccgtgc agtgtggcgt tgtggctgcc
    10921 atggtgtcct actttgtaca aaacggtgcg cgacataaga tcctttaccg agatactttc
    10981 ctgaccatct ttccaacttc cctgggtcca cgacacccaa ttcccgcgtt gggtatcgcc
    11041 gataaaaagc agcacttttt tcgtatttaa aagctcgaat gccagcacga gactgttgtt
    11101 gttgatgccc ctgttcagga ccaaggccag atcttcagcg gagtacagcc aatcttgatc
    11161 gatgcggcgc caggaagcat cgtccgcgct ctcgtctccg atcgccctcg gcgttttgtc
    11221 gtgaccgtaa tgatcactaa aaaatttccc gcattcgggg tgccgaaatg cttccttgag
    11281 cggcacggat aaacgggccg gaaaggtcaa cgtgttctgt tccggttcgc cgtccgccgc
    11341 ccgggcggca gcatagaacg aagcaaaggc cgagattcca tgccccggaa aggcctcgtt
    11401 gccctccgga tcctcgtccg atatcaggtc aggatcgcgc ggtggtccga atgcaaaaac
    11461 gcgcacgcct ttgactcccg ggatgctcat gatttcctgg tgcggtcgaa cgtacttcgt
    11521 attcttcttt ccggcttttt tcttgatcaa gctcattcct tgcttgttga tcgaatctgc
    11581 cggatccttc tttgctgcgg cggcgaattc agttgggtta tcgatcccaa gttctagttg
    11641 aaggagacta tcgagccagg cggctgcttg aattgaagaa gcagccaatc gattgcgagc
    11701 cgccacaagt ccgagcaatt gatcatggtg gcgtttgcga agttcgtttg cgacctcgtc
    11761 gtctggatcc tcagtccatg cgaaccacgc ttctccaatc tcaaagttct tgaaattgtt
    11821 gggaagcccg ttcacatgat cctggtgctc gtgcgttatg atcaccaagt ccaaatgccc
    11881 gcccgtcgac tcgcgaatgt tggcaacaat gttagagatg tcggcaagac cgaaccgggt
    11941 gttcgaaccc ggcttataac cgcagtcgat gagaacgtat gctggctgcc cgttcggccg
    12001 cggaaatgca agcagaaagc aatcgccgtg gccttggcga tacatacgga ccttgacgcc
    12061 agaggccggc gcttgcatca gcccctccgt ctgatggttc acgcccctgg tcgcgccccg
    12121 cgaggattga tgcggacgct tccgttctcg tcgcggcctg gtcttgctgg agcgttgtgc
    12181 agtcctcccg cgtgtatgtg tggtttttcg ttttgccttg ggtggtctct tggacttgcg
    12241 cctggcgctg gatgcctttg acgaactttt tcgagccgcg cttgtcatga ctcactcccc
    12301 tgaatgccga tcagaagagc gaatggctca ttgagccctc gataagccct ttctcttagg
    12361 ctgactgctc tcatgccagc gaaggcattc gcgcttggac ctgcgtctcc ggtagggaac
    12421 tgacgaatac gttcgagctc aatatcgtcg tcgataggcc ccggggttcg gattatgtga
    12481 cgaaaaacat tctgtttagt atcgattatg atggtgcagc cagcgcgata cttgaaatct
    12541 cctttctcct tggcgctgaa tggtgtagac cgcttgtcag ctttctcctg gtcttctttt
    12601 ttgaaatagc cacggcgtcg ctgcgtgatc tccaccacga gatcggtgac aatcgatccg
    12661 tgcgcgtccc ggcgcagcgc cgtgcgaacc gaatgtacct ccaccgtggg ctcgcccgtt
    12721 tttttgcgat acacagtcgc tggcgcatct tttccgatca caaggccgaa cgccttgcag
    12781 tattttcgcc ctctgctgcg atgcagccag ccccacaccg ctcgccgatt ttcctgcata
    12841 tgcttccaga tctcgaggcg atcgctgtcc aaatcccatc gcatcgactg cgcccgagaa
    12901 ccgttcacca cctgcgcttg atcgaagaat accttgatat cttcctggct ttgcttcaga
    12961 gcggctgcaa cggctttctt ggttgagatg cgtgctgttt ttccatgcct gagcttcaac
    13021 tgctgcgccg actctgcaat gacctcggct ccggtgggcc acgtcaaagc ttcaaccgac
    13081 atgctgcggg taccacgcgg atagatgccc cattgtcgga agctttcaac gaatgccact
    13141 cgatatcgaa gctcatcctc tggatcaatg ttgatgtcgg cggtcatcac ggcgcgcaga
    13201 tattccccga aagtgatgtt gacgggcggg caatagtcga tagcgcggat gcacatgcgt
    13261 aagatatttc ccgcgcaagc tgccgcttcc tccgcaagcc gtcttgccat gtccggatgg
    13321 atctcgcctt ccggcagcac gccgcttcct tgcgtcgcaa ttcggtagag atcttcggtt
    13381 cgccgcccat agacgagcag aaacgcgccg aataccgcgg cgactaaaat agccccgcgc
    13441 tcatgcggct ccattgtctt ttcgagtatg gtcgtgtcgg gagggcggcg ctgccatttt
    13501 ccgtccactt ccattcccaa cgcgtcacgc agggcgctgc cgcggccggt tgcgctgcca
    13561 aattgctgcg cgagcttcgc aagcatgttc tcgttctcaa gacgaccacg cgtacgagca
    13621 atctcatcgc gcactacgtc cgtgtaagag aagcgctgaa acagtgcgac gatgtccgcg
    13681 aacgcctcat gaaaggcagg tacatcggga ttgctctgtt cgttgaagcg cggatgtacg
    13741 ccatcaagca gagcgtgggt aacctcgtgc gcgataacgt cgtgggatag acatgcaaaa
    13801 acggtggtcc ccggggtatt gcgggcatca ttggtgcgta ccgggaaata cccaaacaaa
    13861 atcgccttct tgtccgggct gtaataggcg ttttgatcgc gcagagcatg cgggtacatt
    13921 cgaaggcggc ggacgaactc gtgtttccat tggccttcag gattgtctcg aggtgtcacc
    13981 tgacgtggag cccacaaggc gactcggccg agcgcttgct cgaaacgttc gatggtcgcc
    14041 attgcgaccg catagaccat ttgctgatga aattgcggat acccttcaga aggagggagg
    14101 ccgtcctgcg ccaacaaatt tggatcgtcc agatcgactg gtggatacac aactccacta
    14161 gccggatcga catcaactac ttcgatgtat tcgcctatcg gtccaacatc gagacgcccg
    14221 gttttaggat tttgctccca ccgaatcgga agaacgattt cctgaagtgg tgccgtttgc
    14281 agtttctggg caaggctcgg atccaatgca tacactcgaa ggcgacgcac cggaggctta
    14341 tcaaggatag agagcaacat cattccccct gtctatcttg gccgagtttc catactgact
    14401 aggcttctcg tctgctgccg agtgcccaat cgtgcgccag cgcaaccagc cccgccgcct
    14461 ggcggcgaat ctcggcggca tcgatggccc agcgggcgtc gacccgcacg ttgcggccga
    14521 tcgaccaatc caacaccgcc aatccctgca ggaacgcccc gacgcgagcc tgaaatgctg
    14581 gatcgttcga agctgcgggc acgagcacgc cgatatgccg cattttctcg cgctgctgcg
    14641 cgcgcgccgc aagcggccat gccaccgctc cgccgagtgc ggccagcaat tcccgccgtc
    14701 cgatggtgac tgtcatacgc gcctcccgtg cgaggtgcgt aaagggaacg ataccacgtc
    14761 acgagcgttg atcgccccca atcttcctcg ccggtccccc gattgaggcc atttggccgg
    14821 ccacgcggca gccgcgccgc cgagcagcgt gatgaaggcg cggcgtcttg gatcgagcat
    14881 gggctcctcc ccggagcccg ctgtgccagc ttaccgcagg ctcaggatgc tgcggaagca
    14941 cccgcaagtc cttgggctgg acctgaatcg ttctgaacgg gcgggtacgc ggagcgtccg
    15001 gaccttcaaa aaacgacttc gaaaaacagt cggtcttcga tgacttgttc ctttttgacg
    15061 gggctcggcc gatccacgcg cagaaccccg ccgctcgcac gctgctcgag ggccgtgacg
    15121 agttcgggga gaggcatctg ggtccagccc tttctcttcg ccatctcttc gtccaccgga
    15181 agcatggcga cgcgcagccg cttcatcagc tccagcccat agtgcctcag cgtcgcattg
    15241 tggctgccgt gatggccgac cttgtagagg atagtgcggt tgagaagatc ggggccggtg
    15301 acggtcctgc cgcccacggg ccacgcaagg tcctgccacg acagccagtt gcccacttgg
    15361 gcgtccgcgg caaacaggag cacgtcgccg tcggcaagct cgatcgcgag cacgagactg
    15421 gtgttgttgg tgaggctgtc gagctgcagc gcgatgtcgg ggatgcccat gagccagtcg
    15481 ccctcgatgc ggcgccggtc gtcgttacag tagtgatccc gaaaaaacgg cagcctggcc
    15541 gcagtggatt tcgggatctc gaacttgcgg tcgaatggac ggccggcatc gccgccttcg
    15601 agtgcagcgc cgatgccggc catgaagagc ggcagcgcct cgagcccgag cccgtaggtt
    15661 tccttctcgc gcattgacgg cctgatcttg cgcagccgct tctcgcaggg cggtgggccg
    15721 agaacgtaga tccgggcgcc gacgtccggc agctcggtcg gagcctccgc cggcaagcag
    15781 taccggggct tgcgcaccag gctgcgcgcg tgggcgagcg cgtcggaggt cgaggtaccc
    15841 ttcgcggcgc ccaagaattc gaggacgccc gcgatctcga ccgcgccctt ggcgcccgca
    15901 agctgcagct ggctaagacc gagacggagc gcggagagcg ctgcgtccct ttcacgcctg
    15961 agctttctcg ccagctcgtg ttctggatcc tcggtccagg cgagccagag cgcgtgcacc
    16021 ttgagcgggc ggaacgactc cttcgcctgc acgaagccgg acacatgatc ccaatgctcg
    16081 tgggtcacaa ccaggaggtc gatctcgcct tgcgtggtgg cgacgatgtt gtcaacgacc
    16141 tccttcattt ggggccgccg gccatcggca ggcgtgccga ggatcacgcc gcaatcgatc
    16201 attatggaat agggccggct accggcgcgc ggcagcgaca ccaggaaaca gtcgcgcagg
    16261 ccctggcggt acatcctgac cttggcgcgc cagccggggt tggccggcgc gcgcgtcggc
    16321 cggtgcggcg tgcgagtgga cttggtgtgg gacgcgcgag ccaatgcggc cccctagccg
    16381 tacataccgt gcaaggccgc gaacggctcg ctgccgatcg cgtccgccgt gaaatagttg
    16441 ttggctactt cgccggcgcc gccgaacgcc atctgctcgg cgaaacgcaa cgcattaccg
    16501 acgcgcttgc ggacgacgta gcggaccagg gcggagccga aattgatgat gagggtgcag
    16561 ccgccgcgga agcggacacg gggctgctca gcgggcgtga aggtctgggt gagctcgatc
    16621 accacgtccc agtggatgac gcccccggga tcggcgcgcc gggccggacg caccgattga
    16681 accgcgatcc ggctcagctc tccaagatgg gagccgagcc gataaggccc gggccggtcg
    16741 aggcgcacga ggccgagggc ggcgacctcc ttgtccgaaa cttcgcggtc gtcatgcagc
    16801 cactgccaga acagtttcgc gttccgttgt gacaaaagct cggcgttcag tcggtcgata
    16861 ttgaggtccc actcgatcga catgcgttcg agaacgcgcc taatgtcacg caaaggagcc
    16921 gtgggcggct cccacaccag gctgtcgggc gacatgtgct tcgcgtcgcc cggatagatg
    16981 ccgcggtcgc ggaaggccgc aatgaaggcg gtgcgatagc gatgctcgtc gaccgggacg
    17041 aggtcggtat cggccgtgat gagcgcgcgc agatattcac cgaaagtcat gtcgaccggc
    17101 gggcaatagt cgagggcgcg gatgcagatg tctaggatgt gtctggcgac cttgctggct
    17161 tcctgcgcca ggcggttgac gaggtcggcc gggatggcgc ccgctgccag gatttcggta
    17221 ccgccggtcg ccaagcgcag aaggtcccgc gtgcgccgct tgtagatctg caggaacgcg
    17281 tcgaacaccg ctgcgactag gacggcaccg cggtcgtgtg gctcggtggc atcgcggtag
    17341 tcggtctggc tcggttttcg ccgccgccaa acaagccttt gcccgtcggc atcgttcacg
    17401 tcgacatatt cgccgatgta gtcgcgcaac gcgccacggc cggtcgcggc gccaaactgg
    17461 atggccagta gtccgaggag gttctgctgc tcgaggttgc cgcgcgtcgc cgcgatctgg
    17521 tgtctcagcg cctccggcag cgtgaagtgc tggaacaggg ccacgatatc ggcaaacgcc
    17581 tcgtggaacg cccggatgtc gggattggtc ggctcgaaga actgcgcgcg caagccgtcc
    17641 agcagcgcat gcgcggtttc gtgggcgatg atatcatgcg acgtggcggc gaataccgtc
    17701 cccggcatcg tatctgacgc cttaaaatag ccgagcagca gcgacatccg ggcggggctg
    17761 aaataggcgt tccggatgcg tagcgcgtgc ggatagatgc gcagccgctg cacgtattcc
    17821 catcgcacgc cgccgtctct cacctgcacc ctgtgcctcc acagggcgac gcggccgagc
    17881 gcgcgctcga aatactcgat ggtcttcatc gccactgcat agaccatcgg ctggtgaaac
    17941 tgcggcagcg cctccgacgg cgtgaggccc gattgggtca ggatgtgcgg gtgattgagg
    18001 tcgaccggcg cataaaagca attgctcgcc ggatcgtagt cgaccacttc gatatattcg
    18061 ccgaccgggc cgggctggat ttcctcccag gggaccagaa gtgtcgcctc gttgatgtcg
    18121 acggtgtcca tgcgcatggc gagggacggg tcgtaggcga agacgcgcag cgggcgacac
    18181 cgcggcggcg gaacattcac gttgatgtcg agctccagcg cctcggccgg tttctttttc
    18241 agatcggcgt tggctgcgcc aaggcggtca atgtgaggtc tcgccttcgt tgccttcgaa
    18301 cgaagacggg taatgcgcgt tcggttcttc gtctgtttgc gagacaagat cacctcgtcg
    18361 gcacggttga cgcaatccct cagccctcga tcataaggtc gaaaaacagt ggggtctgcg
    18421 tcaggcgttt gcggaatgcg cgccactcgg atgccttggc gcccgccggc ggattgtctt
    18481 gcaggtcgca atctgtctgc aacacccgtc cgcccgcctt ttcctccaaa gcttccttga
    18541 tcgcggggaa gggatgattc cagtcaggct tcggcttgtg gtgcgcccat ttctcgaccg
    18601 ccgtgatcat cgcgacgaac tcgcctctgg tctcgccttg tgcgatccag gagaggttgg
    18661 cgtaattcga cttgagctcg ccgtggaggg tcgcattgtg actgccgtga tgtccaacct
    18721 tgtagaggac cgtgcgggcg agcaggtcct ttgcggtcac ctccgcgttg ccatcggcaa
    18781 acggctgttc cgaccaggag cgccagttgc cggcctgcgc atctcccgcg aacagtagga
    18841 ccttgccgcc ttttgacagc tcgaacgcga gcacgaggct tgaattgttc gtggcgttgc
    18901 tcatggcgag cgccaacgcg gcagcgtccg cggcgcagtc ggcgcctatg cggcgccagg
    18961 ggacgttgtc cggtatgtct gcgtaatcgg cggcatcggc cacgttctga ttgccgaaat
    19021 ggacagtgaa gaaagcgccg aattcgggat gggtgaaagc gccttcgagc gggatcacat
    19081 gccgccgcgg gaacggcgac ttccttgccg atcccccggt ccccacgcct ccgctcgcaa
    19141 ccgcctgggc gtggtcgtcg tccccgaaac tctcctcgcc ctccgggtcg aggtcatcga
    19201 tcttgtcgat gtcatgcggc gggccgagcg cgaaggcacg tgcgctagcc gcccccctga
    19261 tcggcaccgg cttctcatga ggcagcagat aaacagggtc gccgtctgcg caatcccgca
    19321 gaaacttcat cgcaagcttg ttgaccgaag aagcgggatc ctttccggcg gcgcccaggc
    19381 ccgtccccct gaacccgcgg gggtcctcgc ccagctcgaa ttccaggaaa tcagccactt
    19441 tgtcggcggc ctcggtgagg gcgagcctgt gcaactgcga gcttgcgtcg atcaggccga
    19501 gcaaacggtc gttgaacttc ttccgcagct tgttggcaat tgcgtcgtgc ccatcctcgg
    19561 tccaggcgaa ccaggcctcg ccgactttca attccggaaa atttttcgcc gtcaaaccgt
    19621 tcacgtgatc ctgatgctcg tgcgtgacaa tggcgacatc gacgaagccg ccggtcgtgg
    19681 cgatgatgtc cgctccgata tccttgacct ttgtggggtt ggcgagctcg gccggcgagc
    19741 ccggtttgta gccgcaatcg ataagcacgt aggcgggctt gtcaggcttc ttgccggcga
    19801 aggcgatgag gaagcagtcg ccgtgaccga tgcgatacat gcgaactacc gccccgccgg
    19861 gcggcggctt gagtgggctc acggcagcgg ctgcgggctt gcgctttgtt tgtttcgcca
    19921 tcagtttacg ctccaaagcg agaatgcagg agcgcgaacg gctcggcgtc tggcttcaat
    19981 cgcgctgcgg ccccatcaaa agcgttgggc ggctcgagcc cgcgcacgaa atagcttcgc
    20041 aggcgatcga attcgcgctc gtcggcaact gttccgggcg tgcgcaccac gcgcttgatc
    20101 tgtgtgcgct cggggtcgat caaaagagtg cagcccgcgc ggtacttgaa atcgggttgc
    20161 ggatccttcc aggcatcggg gattgcatcc ttttgcacct gcgtttccgt atcgaagtaa
    20221 ccaatgcgcg tctgtgtcac gacgaccaca aggtgctttt ccggccatcc cagcgcgccg
    20281 cgcttcatca ccgtccgcac cgaatggatt tccacaagtg gacgaccacc ccgttggcgt
    20341 atggtcatcg ggaggctgga tggcgtgacc acaagaccca ggcaattggc aagccactcc
    20401 gcatcttcat cgagcagcca gttgtggaac aaggccgctt gcctctcgcc ttccagccag
    20461 atctggagtc gagacagcga caggctcacg ggcgtcagca ccttttcaga tgcgcccgga
    20521 acaatcgatg caggaggtgt gaccttgctt gcgtagtatt cgtccttggg cttggcgccc
    20581 agatccagga acattttctt gaccgcgcta ctgccgcgcg cggtagccat cctcagatcg
    20641 gcatgcacaa ggtcctgcgc ggcgggagtc gccgtactag ccgtcacctg ttccgcgatg
    20701 acctcctcct ccgtcggcca gagcaggctg tcctccgcca tggacaggac gccgcgcggc
    20761 gaaattcccc attcgcggaa gctttcgatg aacgcgagac gccagttctc cacgtcggcc
    20821 ggatacatgt cgagatcggc ggttacgatc gcgcgcagat agtcgccgaa agtgatgccc
    20881 accggcggac aataatcgat ggcgcggatc gacatctgca aagcgcggtc ggcgcagcgc
    20941 tgcgcctcct ccgccagccg gttgacgagg tcggggtgaa tatcgccctc gcgcatgatg
    21001 ccggtgcctt ccgaggcgat gcggaacaga tcgcgcgtgc ggccgatgta gaccttcatg
    21061 aaggcgccga acaccgccgc cacgagaatg gcaccacgat cgtgcggttc ggaggttttc
    21121 gcgagcagga acggatcggg ctttttcggc tgccatttgc ccgaggcgtc cttgccgccg
    21181 aggtagcttc gcagcgcgtc gccacggccg gaggcgagcg caaattgctg cgccagctgt
    21241 ccgagcacgc tctcgcgcag cagatcgccg cgggtccgcg caatctgatc gcgcagcacg
    21301 ccgggatagg tgaaatgctg gaacagcgcg acaatgtcgg cgaaagcctc gtggaacgcc
    21361 agcacgtcct cgttgaccgg ctcgttaaag cggggatgca cgccgtccag caatgcgtgg
    21421 gtggtctcgt gcgcgatgat gtcgtgggag aggcatgtaa atacggtcgt gccggccgtg
    21481 ttagcgccgt tcctcgccga ggccgggaaa tacccgaaaa gcagggcctt cttctgcggg
    21541 ctgtaatagg cgttctgctg gcgcatcgca tgcgggtaga tgcgcaggcg cggcacgtga
    21601 ttttcgacgt atttcctctc cgccgtctgg tagcggtacg tcgaccagag cgcttttcgg
    21661 ccaagagcgc gctcgaactg ctcgatggtc ttcatggtca ccgcatagac catctgctgg
    21721 tgaaagcgcg ggtcggattc ggaaggctgc agcccatccg tcgccagcag gtccgcggtg
    21781 ttgagatcga cagggccgta aacggcgccg gcggccggat ccacgtccac gacttcgacg
    21841 tagccgccga tgggacccgg gcgcaggttg tcctcccagg gcacctcgat tgtcacgctg
    21901 ctcgtgccga cgaggtcgta ccgcgcagcc agcgccggat cgaaggagta tatccgtaat
    21961 ctgcgcgcgt tcggccgcgg ataacgcacg atggttgtca tatcgacggc cccctccggt
    22021 gttgtttttc ctcgagatac cgcgacgggc cggcaatgag tgtgaactaa gtcacacttt
    22081 ctgcttggtt ccgccggtaa cgcgtaggtg tgtcgagaaa cgatcaggct tgtacgatct
    22141 tgcttgtaca gcgggcggga cccgtcgtct tgccggcgca gggaaccgtc cggaatggga
    22201 ctgcgggagg cgcggaccag ccgctggcag tactgtcgag atgtcgtcca gttggttctc
    22261 ggcagtagct aagacatcag ctcctgcaac tgcaagagca ggtgtgggga gcgaccaagc
    22321 gccgtgagac catccttgtc cggcgtgatg agtcaccgct gcgctagtcg gatgcgtgat
    22381 cccagtcctg ccatgcctgt ttcccggccc aaacgtgtag acgagccggg tatacccggc
    22441 aggacgcact ggcgccacgc ttcgggatcc catatcggca ccatgatgcg gtaccacgac
    22501 cccggaagta ttcccggtat gggcaccgcg acgcgaccgc aatcctgatc gcctaccgac
    22561 acggtcttcg cgccagcgaa ctctgcgaat tgacctggaa catgatcgag cttaatagcg
    22621 gccgaatcca tgtgcggcgg gccaagaacg gcgtcgatag cacgcatccc ctcaccggca
    22681 aggaaatccg ggcactgcgc cagctgcgac gcgagaacct gcaaagccgc tacgttttca
    22741 acaccgaacg cggcgggccg gtcacgaggg cctggttcct caaaatggtc cgccggactg
    22801 gggagttggc caaacttccc ttcccgattc atccgcacat gctgcgccat ggctgcggtt
    22861 tcaggttagc caatgacggg gtcgataccc gcgcgctgca acacttccta ggccaccgca
    22921 acatcatgca cactgtgcgg tataccgagc tgcgctccga ccgcttcgac gggttctgga
    22981 aggattgatc cattgtcgta ggatcgaggc cggcgatgtg ctgatcgtca cccagctggc
    23041 cgcggtcggg catcgaggtc cgcattctga ccgcgatctc gtgatttaca gctggcacgc
    23101 gtgggtcgtc gttgatggcg atgagattgc cggtttttcc ttgtgcggct caggcgatgt
    23161 tcgataagac catcccgctg gctaagctgc gtttcaaagc aacacctttt ggaattgcga
    23221 atgaaaagaa cctccttgac ccaccccttg cagatcgccg tcgtaaccac tgggcccaag
    23281 ttcgggcgtg tcggaattac cttttgcccg ggacagtatg accgacacgc tatgagtgga
    23341 tatcaggatc gggatctagc cctcgatctc gacacggtcc tcgattgggg agccgccgcg
    23401 gtggtcacac
//
LOCUS       DATLJT010001409         5058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_807193,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001409 DATLJT010000000
VERSION     DATLJT010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807193"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1441)
                     /locus_tag="VM910_32395"
     CDS             complement(74..1441)
                     /locus_tag="VM910_32395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HVI67243.1"
                     /translation="MDLNRRHILGASAAGMAGALAATDPARAAPLTSALGRDATQYGV
                     QPGSTDDQTRALQHAIDEAARSQNPLALPPGVYRTGSLHLQHGIQLVGVRGATKLQFT
                     GGTSLLVCEGADHIGISGITLEGGSIPLPARRGLIHCVGGRDLRIADCEIANSGGAGI
                     WLEQISGAVGGNIITNTAVTAIVSFDAQGLIVARNGIFGTNDNGIEILRTAIGDDGTL
                     VADNRIEDIKAGPGGSGQYGNAINAFRAGNVIVRGNRIRNCDYSAVRGNSASNIQITN
                     NSVSDVREVALYSEFSFEGAVISGNTVDGAALGVSVCNFNEGGRIAVVSGNIIRNLLA
                     KRPIGTAPDDDGGIGIYVEADTSVTGNVIENAPAFGIVAGWGKYLRDVAITGNVIRNA
                     FAGIGVSVVAGAGTAVVNNNMISETPRGAVVGLDHNSAITTDLSAEGAQRYAQVVIGT
                     NAMRR"
     gene            1614..2525
                     /locus_tag="VM910_32400"
     CDS             1614..2525
                     /locus_tag="VM910_32400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI67244.1"
                     /translation="MGSMRHRGQAASIVRRTLFEGAAIGISSFAAHPESDACGDVERQ
                     NRNVVVLPFSGVFAKHDAPGRHVVGTPSHAVFIASDTPYRVAFPGAVGDRAIILRFDD
                     ALLPEEVDRRRCGDALGSQGLLTVEAMMRRDLLRRRLTDTTAERLEIETLGLELLSLC
                     LLVLRHKHMPNRAAARQRRLRAVERVKEAVAVAPAEAWSVARLAAVANLSPFHLCHVF
                     RELVGASIHEYVLRERLAQALTAVLDSGDDLTGIALEAGFASHSHFTARFRNFFGCTP
                     SALRQKKGVGQISQLRKIVTARTSDRD"
     gene            2588..3484
                     /locus_tag="VM910_32405"
     CDS             2588..3484
                     /locus_tag="VM910_32405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67245.1"
                     /translation="MDIATFHARRRFAEVKSGRIAYVEDGEGPPALFVHGVPLNGYHW
                     RHVIDRVRHRRRCIAIDLMGLGYSEIAPAQDVSFTAQARMLAEVLDALDIERVDLVGN
                     DSGGAIAQIFAAHNPDRLNSFVLTNCDVHDGWPPPQVLPLMKHARAGTIASIFQPMIE
                     RPDLARQRFAGGESVPLFRSYADPGVLTDEVIRLYLQPLFSSPRRIDAFQRYWLGFDN
                     VHTVAIHDALKALQVPTLIVWGLQDFFFDKKWAYWLRDTIPGAREVIEVNDARLFFPE
                     DRPDSLTEPMLRFWNELSKAQG"
     gene            complement(3489..4481)
                     /gene="meaB"
                     /locus_tag="VM910_32410"
     CDS             complement(3489..4481)
                     /gene="meaB"
                     /locus_tag="VM910_32410"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HVI67246.1"
                     /translation="MVPQASSRFDIKTLAKTLRAGDRAGLARAITLIESRRSDHRAAA
                     RELVQTLLPATGKAIRVGITGSPGVGKSTTIDALGSFLIEGGHKVAVLAVDPTSARTG
                     GSILGDKTRMARLAASDSAYIRPSPSSGTLGGVAAKTREAMLLCEAAGFDVILVETVG
                     IGQSETAVSDMTDFFLALMLPGAGDELQGIKKGLVELADMIAINKADGDNIKRANLAA
                     ADYRSALHILQPRSEHWQPPVVTYSALTGAGIAGLWQKIVDHRNAMTASGDFAARRRQ
                     QQVKWMWSMLEQRVLVRLRADTSTRARVKKTEAEVAAGHLTPALGAEQIAGMLR"
     gene            complement(4578..>5058)
                     /locus_tag="VM910_32415"
     CDS             complement(4578..>5058)
                     /locus_tag="VM910_32415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085838.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVI67247.1"
                     /translation="AMSDKVEKLQALIDAFEEAEGRRPRILVAKIGQDGHDRGQKVIA
                     SAFADVGFDVDIGPLFATAEEAARQAVENDVHILGVSSLAAAHLTAVPELKAALKKHG
                     REDIMIIVGGVVPPQDYDALYKAGAEAIFPPGTVIADAAEELIHKLNARLGHSEAAE"
BASE COUNT          819 a         1619 c         1686 g          934 t
ORIGIN      
        1 tttccgccga cacggctgga cgtggtgacg atcgagcagc gatatccgct gttgcgcaat
       61 tccctttaag ggattaccgc cgcatcgcgt tggtgccgat caccacttgc gcgtagcgtt
      121 gggcgccctc ggccgacagg tccgtggtga ttgcgctgtt gtgatcgagc ccgaccaccg
      181 cgccacgggg cgtttccgaa atcatgttgt tgttgacgac cgcggtgccc gcgcccgcaa
      241 ccacggacac gccgatgccg gcaaacgcgt tgcggatcac attgccggtg atggcgacgt
      301 cgcgtagata tttgccccag cccgcaacaa tgccgaaggc gggagcattc tcgatcacat
      361 tgcccgtcac cgaagtgtcc gcttcgacat agatgccaat gccgccgtcg tcgtcgggcg
      421 cagtgccgat cgggcgcttc gcaagcaggt tgcggatgat gttgccggaa acgaccgcga
      481 tgcgaccgcc ctcgttgaaa ttgcagacgg acaccccgag cgccgcgccg tccacggtat
      541 tgcccgatat caccgcgccc tcgaacgaaa attccgagta gagcgcgacc tcgcggacgt
      601 cgctgacgct gttgttggtg atctggatgt tggaagcgga attgccgcgc acggccgaat
      661 agtcgcaatt cctgatgcgg ttgccgcgca cgatcacatt gcccgcgcga aacgcgttga
      721 tggcgttgcc atattgcccg gagccgccgg gacccgcctt gatgtcctcg atgcgattgt
      781 cagcgaccag cgtgccgtcg tcgccgatcg cggtccgcag gatttcgatg ccgttgtcgt
      841 tggtgccaaa aatgccgttg cgcgccacga tcaggccctg cgcatcgaac gagacgatcg
      901 ccgtcacggc tgtgttggtg atgatattgc cgccgaccgc gccggagatc tgttcgagcc
      961 agatgccggc gccgccgctg tttgcgatct cgcagtcggc gatgcgcagg tcgcgtccgc
     1021 caacacagtg gatcaggccg cgccgggccg ggaggggaat gctgccgccc tcgagcgtga
     1081 tgccgctgat cccgatgtgg tcggcgcctt cgcacaccag cagcgatgtg ccgccggtga
     1141 attgaagttt ggtcgcgccg cgcacgccga cgagttggat gccgtgctga aggtgcagag
     1201 agccggtgcg gtagacgccc ggcggcagtg ccagcgggtt ttgtgagcgc gccgcctcgt
     1261 cgatcgcgtg ctgcagcgca cgggtctggt cgtccgtgct gccgggctgc acgccatatt
     1321 gtgtcgcgtc gcgccccagc gccgatgtca gcggcgcagc acgtgcagga tccgtcgcag
     1381 caagggcgcc ggccatgccg gccgcggaag ccccgagaat atggcggcga ttgaggtcca
     1441 tgaaccgctt gctctttttt cgaaggcgct cgagccctat gaagtaccgc agaaatgcgg
     1501 gcgcgagcgg cgggcgcagg cgattgttgg ggggtagggt taatggttcg gcatgatatg
     1561 gcacgccgcg gcgtccttgc cggtccgttg cgccgtggct gagggcgtgc atcatggggt
     1621 ccatgaggca tcgaggtcag gctgcatcga ttgtccggcg cacgctgttc gagggcgccg
     1681 cgataggcat tagttcgttc gcggctcatc cggagtcgga tgcctgcggt gatgtagaac
     1741 ggcagaatcg gaatgtcgtg gtgctgccct ttagtggcgt cttcgccaag cacgacgcgc
     1801 ccggccggca tgtggtgggc acgcccagcc atgcagtctt catcgcatca gatacacctt
     1861 accgcgtcgc ctttccgggc gctgtcggcg atcgggcaat catcctccgg ttcgacgatg
     1921 cactgctgcc cgaagaggtt gatcggcggc ggtgcggtga tgcgttggga tcgcagggcc
     1981 tcttgacggt cgaggccatg atgcgccgcg atctgcttcg gcgccgcctg accgacacga
     2041 ctgcagagcg gctcgagatc gagacgctcg gcctcgaact cttgagcctg tgtctgctcg
     2101 tgctgcgcca taagcacatg ccgaatcggg ccgccgcgcg ccagcggcgg ctgcgtgcgg
     2161 tcgagcgcgt gaaggaggcg gtagcggtgg cgcccgccga ggcgtggagc gtcgccaggc
     2221 tggcggcggt tgccaacctt tcaccctttc atctgtgcca cgttttccgc gaattggtcg
     2281 gcgcctcgat ccatgagtac gtgctgcgcg agcggctggc gcaggcgctc accgcggtgc
     2341 tcgacagcgg cgacgatctc accgggatcg cgcttgaggc gggttttgcc agccacagtc
     2401 acttcacggc ccggttcagg aacttcttcg gttgcactcc atccgcgctg cgacaaaaga
     2461 aaggcgtcgg acaaatcagc caactccgca agatcgtgac agcccgcacg agtgaccgag
     2521 actagccttg cttgaggcag tcagattgga cggcgactcg ccggacattc gggtcacgag
     2581 gcacagaatg gacatcgcta cctttcatgc gcgccggcgc tttgccgaag tcaaatccgg
     2641 gcggatcgcc tatgtcgagg acggggaggg accgccggcg ctcttcgtgc acggcgtgcc
     2701 tcttaacggc tatcactggc gccatgtcat cgaccgggtg cggcaccgga ggcgctgcat
     2761 cgcaatcgac ttgatgggcc tcggctactc cgagatcgca cccgcgcagg acgtctcctt
     2821 caccgcgcag gcgcgcatgc tcgccgaggt gcttgatgcg ctcgacatcg aacgggtcga
     2881 tctggtcggt aacgacagcg gaggcgcgat tgcgcagata ttcgcagcgc ataatccgga
     2941 ccggttgaac tcattcgtgc tgaccaattg cgacgtgcac gacggctggc cgccgcctca
     3001 ggtcctgccg ctgatgaagc acgcgcgcgc cggcacgatt gcgtcgatct tccagccgat
     3061 gatcgagcgg cccgacctcg cgcgccagcg ctttgccgga ggcgagtccg tgccgctgtt
     3121 ccgcagctat gcagacccgg gcgtgctgac tgacgaggtg attcggctgt atctgcagcc
     3181 tctgttttcc tcgccacggc ggatcgatgc cttccagcgc tactggcttg gtttcgacaa
     3241 tgtgcacacc gtcgccatcc atgacgctct gaaagcgctg caggtgccga cgttgatcgt
     3301 gtggggcctg caggacttct tcttcgacaa gaagtgggcc tactggctgc gcgacaccat
     3361 ccccggagca cgcgaggtga tcgaggtcaa tgacgcccgg ctgttcttcc cggaagacag
     3421 gccggatagc ctcacagagc cgatgctgcg gttctggaac gagctgtcga aggcgcaggg
     3481 ctgatctgtc agcgcaacat cccggcgatc tgttctgcgc caagagccgg cgtgaggtgg
     3541 ccggcggcga cctcggcctc ggtcttcttg acccgcgcgc gggtcgaggt atccgcgcgc
     3601 agccgcacca gcacgcgctg ctccagcatc gaccacatcc acttcacctg ctgctggcgc
     3661 cgccgcgcgg caaaatcgcc ggaggccgtc atcgcgttgc gatgatcgac gatcttctgc
     3721 cagagtcccg caattcctgc accggtcaac gccgaatagg tgacgaccgg cggctgccag
     3781 tgctccgagc gaggctgcag gatatgcagc gcgctgcgat aatcggcggc ggcaagattg
     3841 gcgcgcttga tgttgtcgcc atcggccttg ttgatcgcga tcatgtcggc aagctcgacc
     3901 agccccttct tgatgccctg caactcgtcg ccggcacccg gcagcatcag cgcgaggaag
     3961 aagtcggtca tgtcgctgac cgcggtctcg gattgtccga tgccgacggt ctcgaccagg
     4021 atcacgtcga agccggcggc ctcgcagagc agcatcgcct cgcgcgtctt cgccgcgacg
     4081 ccgccgaggg tgcccgagga gggcgagggg cggatgtagg cggaatctga cgccgccagc
     4141 cgcgccatcc gcgtcttgtc gccgaggatc gagccgccgg tgcgtgccga ggtcggatcg
     4201 accgccagca cggcgacctt gtggccgcct tcgatcaaaa aactgccgag tgcatcgatg
     4261 gtggtggatt tgccgacgcc gggcgagccc gtgatgccga cgcggatcgc ctttccggtt
     4321 gcgggcaaca gcgtctgcac cagctcacgg gccgcagccc gatgatcgct gcgcctgctc
     4381 tcgatcagcg tgatcgctcg cgccaggcct gcgcgatcgc cggcgcgcag cgttttggcc
     4441 aatgtcttga tatcgaagcg ggagctagct tgggggacca tggcctgctt tacaacggca
     4501 ggccggaaca ggcgaggggc cgggccaagt atgccgctcc tggacgagcc cgatcccggg
     4561 gtcgaacaga cgttgcgtta ttccgccgcc tcgctgtggc cgagccgggc gttgagcttg
     4621 tggatcagct cttcggcggc atccgcgatc acggtgccgg gcggaaagat cgcttccgcg
     4681 ccggccttgt agagcgcgtc ataatcctgc ggcggcacca cgccgccgac gatgatcatg
     4741 atgtcctcgc ggccgtgctt cttcagcgcg gccttcagtt ccggcacggc agtgagatgc
     4801 gcggccgcaa gcgaggagac gccgagaata tgcacatcgt tctcgacagc ctggcgcgcg
     4861 gcttcttcgg cggtcgcaaa cagcggcccg atatcgacgt cgaagccgac atcggcgaag
     4921 gccgaggcga tcaccttctg gccgcggtcg tggccgtcct ggccgatctt cgccaccaga
     4981 atgcgcggcc gccgcccctc ggcctcctcg aaggcgtcga tcagcgcctg cagcttttcg
     5041 accttgtcgg acatggcg
//
LOCUS       DATLJT010001410         1512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1730471,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001410 DATLJT010000000
VERSION     DATLJT010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730471"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1399)
                     /locus_tag="VM910_32420"
     CDS             complement(53..1399)
                     /locus_tag="VM910_32420"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HVI67248.1"
                     /translation="MKTTHVEHEDWSEDIFEPDDAPTGDGKANGKAGASASGNAWPVL
                     DAAAYHGLAGEVVTTILPHTEADPVALLLQTHVSFGNVVGRQPYYFIEGAEHYPVLFA
                     LLAGPTAKGRKGTAANRIRPIFDVADCDWAHNNVTSGISSGEGILHAIHDDIYGTDKK
                     TGLQVLVEAGIEDKRLLLDEREFSAALDSMRREGNVVSRIIRDAWDCPRVLRTLTKHN
                     PTKVTCPHISVIGQITIDELQRKLDQSAMMSGFANRFLYACTRRSKLLPHGGTLHPDD
                     EIRLGRAMLAAVCAARTLTRVTMTPAAAELWNAIYPKLSADVPGLLGAATARGDAQTL
                     RLSMLYALLDRAPKIDAVHINAALALWNYCEASARYIFGDFTGDPVADVILRALRTAG
                     AAGMSRSDISHNLFSRNTDAAKMQAALGQLLAAGKVRCDKQKLNGPGRARELWFAV"
BASE COUNT          245 a          487 c          493 g          287 t
ORIGIN      
        1 tgaccaacgc tcgcgctcgg tgtctttcca ggccatgatc cttgcgcttc tttcacactg
       61 caaaccaaag ctcgcgcgcg cggccggggc cgttgagctt ctgcttgtca cagcggactt
      121 tgccggcggc gaggagctgg ccaagcgcag cctgcatttt ggcggcatcc gtattgcggc
      181 tgaacagatt gtggctgatg tccgagcggc tcatcccagc ggcaccagcg gtgcgcagcg
      241 ctcgtaggat gacgtcggcg acggggtcgc cggtgaagtc tccgaagatg tagcgcgcgg
      301 atgcctcgca gtaattccac agcgccaacg ccgcattgat gtgaaccgcg tcgatcttcg
      361 gcgcccggtc gagcaacgca tagagcatgg acagccgcag ggtctgcgca tcaccgcggg
      421 cagtggcggc gccgagcagc ccgggcacgt ccgccgagag cttggggtag atcgcgttcc
      481 acaactcggc cgctgccggc gtcatggtga cgcgcgtgag tgtgcgcgcc gcgcacaccg
      541 ccgcgagcat tgcacgaccg agccggattt catcgtcggg atgcagcgta ccgccatgcg
      601 gcagcagctt gctgcggcgg gtgcaggcgt agagaaaacg gttagcaaat ccgctcatca
      661 tcgcgctctg gtcgagcttg cgctgcaact catcgattgt gatctggcca atgacggaaa
      721 tgtgcggaca cgtcactttc gtgggattgt gcttggtcag cgtccgcagc actctggggc
      781 aatcccaagc atcgcggatg atgcggctga cgacgttgcc ttcacgccgc atgctgtcga
      841 gcgccgccga gaattcgcgc tcatcgagca gtaaacgctt atcctcgatg ccggcctcga
      901 ccaggacctg caggcccgtc ttcttgtcgg tcccgtagat gtcatcgtgg atcgcgtgca
      961 ggatgccctc gcccgagctg atgccgctgg tgacgttgtt gtgcgcccag tcgcagtcgg
     1021 cgacgtcaaa tatcgggcga atgcgatttg ctgcggtacc tttgcgcccc ttcgccgtgg
     1081 ggccggcgag cagggcgaag agcacgggat agtgctcggc gccttcgatg aagtagtacg
     1141 gctgccgccc gaccacgttc ccgaagctga cgtgagtttg cagcagcaac gccaccggat
     1201 cagcttcggt gtgcggcagg attgtcgtca ccacctcgcc ggcgaggccg tggtaggcgg
     1261 ccgcgtccag caccggccag gcattgccgc tcgcgctcgc accggccttg ccgttggcct
     1321 tgccgtcgcc ggtcggtgcg tcgtccggct caaatatgtc ctcgctccaa tcctcgtgct
     1381 cgacgtgcgt ggtcttcatg acatcaccac gtcgttaaaa tctttgaccc gcttggtcgt
     1441 gagcagccgc acgcggcaac cggcagcgac gtagcgcgcg gcgcacgcct cggcatcggg
     1501 gcgcgccacg cc
//
LOCUS       DATLJT010001411         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1270557,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001411 DATLJT010000000
VERSION     DATLJT010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270557"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VM910_32425"
     CDS             <1..302
                     /locus_tag="VM910_32425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67249.1"
                     /translation="QALVIGFMALAFFGKGIGALGWAVVSDTSPKEAGGVSGGLFNMF
                     GNLSSITTPIIIGYILAATGSFNGALVFVGANALMAAVAYLVIVGKIERVRLKTA"
     gene            418..1323
                     /locus_tag="VM910_32430"
     CDS             418..1323
                     /locus_tag="VM910_32430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI67250.1"
                     /translation="MFDLNQLRCFVTVAEELHFGRAAARLNMTQPPLSRQIQVLEHII
                     DATLLERTSRSVRLTPAGRSFLPEARRILKLAESASQVARRIAMGKTGSLKIGYTAAA
                     AYGFLPDLIAACRARLPEVDFSLKEMVSGEQLEALATGQIDAGLLRPPIARPEFATRR
                     VVAEPLLAAIPKKHPLASVEAIAIKDFDDQPFVMYSPYESRYFHDLVVALFTRADVLP
                     RYVQHVGQIHSILAMVRAGLGVAIVPAAAASLRIADVRLRPLKLRTQTSVELFMVWRR
                     DYENPLLPTLVEIGGELASIHGHDH"
     gene            1449..2393
                     /gene="kdgD"
                     /locus_tag="VM910_32435"
     CDS             1449..2393
                     /gene="kdgD"
                     /locus_tag="VM910_32435"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HVI67251.1"
                     /translation="MSKMTPQEMAAKIGSGLLSFPVTPFKADFSFDEATYRANMDWLC
                     GYEVAGLFAAGGTGEFFSLTPTEVAQIVAVAVDETNGRVPVLAGTGYGTAIAREIAVA
                     AEKAGADGLLLLPPYLTHSEQAGLAAHVEAVCASTRLGVIVYNRDNAILQPDTLARLC
                     ERCPNLVGYKDGIGDIELMTRVYSKLGDRLTYIGGLPTAETFALPYLDMGVTTYSSAV
                     FNFVPEFATRFYAAVRRRDHQAIHAGLKDFILPLIAIRNRKKGYAVSMIKAGMKVIGR
                     DSGPVRPPLTDLTEAEIGELAELVQNLPVAAWAKQAAE"
     gene            2484..>3442
                     /locus_tag="VM910_32440"
     CDS             2484..>3442
                     /locus_tag="VM910_32440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI67252.1"
                     /translation="MQVIPVAGHDSMLLNLSGAHAPFFTRNIVVLTDRSGHTGVGEVP
                     GGEKIRQTLQDARDLVVGQSIGALNNILASMRSAFADRDAGGRGKQTFDLRVMIHAVT
                     AVESALLDLLGQHLSLPVAALLGEGQQRKTVETLGYLFFVGDRRKTSLPYLAGENQTI
                     DWFRLRHQEAMTPEAVVRLAEATHDHYGFADFKLKGGVLPGEREIEAVTAIAKRFPKA
                     RVTLDPNGAWSLDEAIRLCKDMHGILAYAEDPCGAEAGFSGREIMAEFRRATGLPTAT
                     NMIATDWRQLAHALRLGAVDIPLADPHFWTMQGSVRVAQTCRD"
BASE COUNT          593 a         1143 c         1071 g          635 t
ORIGIN      
        1 cccaagcgct cgtcatcggc ttcatggcac tcgccttttt cggcaagggc atcggcgcgc
       61 tcggctgggc cgtggtctcc gacacatcgc cgaaagaggc gggcggcgtc tcgggtggcc
      121 tgttcaacat gttcggcaat ctctccagca tcacgacgcc gatcatcatc ggctacatcc
      181 tggccgcgac cggctccttc aacggtgccc tcgtgttcgt cggcgccaac gcgctgatgg
      241 cggcggtcgc ttatctcgtc atcgtgggca agatcgaaag ggttcggctg aagacagcct
      301 gagtgcactt ttacctctgt tctgcaaggc ggcggcgttc ggccgccgcc tttgttttgg
      361 gagccatcga tgctagaaat caggaacaga cgcctcgtcc attccgagct tgtatcgatg
      421 ttcgacctga accagctccg ctgttttgtc acggtcgcgg aagagctgca tttcggacgc
      481 gcggcggcgc ggttgaacat gacccaaccg ccgctcagcc ggcagatcca ggttctcgaa
      541 cacatcatcg acgcgacgct gctcgagcgt accagccgct cggtccggct gacgcccgcc
      601 ggccgcagct tcctgcccga agcgcggcgc atcctcaaac ttgccgagag cgcctcgcaa
      661 gtcgctcgcc gtatcgcgat gggcaagacc ggctcgctca agatcggata cacggcggcc
      721 gcggcctacg gcttcctgcc cgacctgatc gccgcgtgcc gggcgcggct gcctgaggtc
      781 gacttctcgc tgaaggagat ggtgtccggc gagcagttgg aagcgctggc gaccggacag
      841 atcgacgccg gtctgttgcg gccgccgatc gcccgtccgg agtttgcgac aaggcgcgtg
      901 gtcgccgagc ccctgctcgc ggcaatcccg aagaagcatc cgctcgcctc ggtcgaggcg
      961 atcgcgatca aggatttcga cgaccagccc ttcgtgatgt attcgcccta cgagtcgcga
     1021 tatttccacg atctcgtggt cgcgctgttc acgcgcgccg acgtcctgcc gcgctacgtg
     1081 cagcatgtcg ggcagatcca ctccatcctc gcgatggtgc gcgccggtct cggcgttgcc
     1141 atcgtgccgg ctgcagccgc aagcctcagg atcgcggatg tcaggctgcg gccgctgaag
     1201 cttcgcaccc aaacctcggt cgaactgttc atggtttggc ggcgcgacta cgaaaacccc
     1261 ctgctgccga cactcgtcga aatcgggggc gagctcgcgt cgatccacgg ccacgaccat
     1321 tgatgccaat tgcgcatcga tcgatccagc gtttggcttg gacgcgcatc gaaagagtca
     1381 atagaaatct cccatcccga cgcgatgaat gcgcgtcccc tgaccagaat atttagagga
     1441 gtcccgctgt gagcaagatg accccgcagg agatggccgc gaaaattggc agcggcctcc
     1501 tgtcctttcc cgtcaccccg ttcaaggcgg atttttcctt cgatgaggcg acctaccgcg
     1561 cgaacatgga ctggctctgc ggctacgagg tcgcgggcct gttcgccgcc ggcggcaccg
     1621 gcgagttctt ttcgctgacc cccaccgagg ttgctcaaat tgttgcggtc gcggtcgacg
     1681 agaccaacgg ccgcgttccc gtgctcgccg gcacgggata cggaaccgcc atcgcacgcg
     1741 agatcgccgt ggcagcggag aaggccggcg ccgacgggtt gttgctgctg ccgccttatc
     1801 tcacacattc cgagcaggcc ggccttgccg cccatgtcga ggcagtctgc gcgtccacca
     1861 ggctcggcgt catcgtttat aaccgcgaca acgccatcct gcagcccgac acgctggcgc
     1921 ggttgtgcga acgttgcccg aatctcgtcg gctacaagga cggcatcggc gacatcgagc
     1981 tgatgacccg cgtctattcg aagctcggcg accggttgac ctatatcggc ggcctgccca
     2041 ctgcggaaac cttcgccctg ccctatctcg acatgggcgt caccacctat tcctccgcgg
     2101 tgttcaattt tgtgccggag tttgcaacgc gcttctatgc ggcggttcgt cgccgcgatc
     2161 accaggcgat ccacgcgggc ttgaaggatt tcatcctgcc gctgattgcg atccgcaatc
     2221 gcaagaaggg ctatgccgtt tccatgatca aggccggcat gaaggtgatc ggccgcgatt
     2281 ccggaccggt ccgcccgccg ctcacggacc tgacggaagc cgagatcgga gaacttgcgg
     2341 aactcgtgca gaacctgccg gttgcggcat gggcgaagca ggcggcagaa taacggccgg
     2401 ccgcggccaa cgagccaagg gaggaacagg tgatccagga agcggttcgc gccggagttt
     2461 ccggtgcccc cgttgtcacc gagatgcagg tgatcccggt tgccgggcac gacagcatgc
     2521 tcctcaatct ctccggcgcg catgcgccgt tctttactcg caatatcgta gtcctcaccg
     2581 atcgttccgg ccacactggc gttggtgagg tgcccggtgg cgaaaaaatc cgccagacgc
     2641 ttcaggatgc tcgcgatctc gtcgtcggcc agagcatcgg cgccctcaac aacatcctgg
     2701 cgtcgatgcg cagcgccttc gccgaccgcg acgcaggcgg gcgcggcaag cagacgttcg
     2761 acctgcgcgt gatgatccat gccgtaacgg ccgtcgaatc cgcgctgctc gacctgctcg
     2821 gccagcattt gtcgcttccg gtcgccgcac tgctcggcga gggccagcaa cgcaagaccg
     2881 tcgagacgct cggctatctc ttcttcgtag gtgaccggcg caaaaccagc ctgccctatc
     2941 tcgcgggcga gaatcaaacg atcgactggt tccgcctgcg ccatcaggag gcgatgaccc
     3001 cggaagcggt ggtgcggctc gcagaagcta cgcacgatca ttacggcttc gcagacttca
     3061 agctgaaggg cggcgtgctg ccgggcgagc gcgagatcga ggcggtcacc gccatcgcca
     3121 agcgctttcc gaaggcgcgc gtgacgctcg atccgaacgg cgcctggtcg ctggacgaag
     3181 cgatccgtct ctgcaaggac atgcacggga tcctcgccta tgcggaagac ccttgcgggg
     3241 cggaagccgg tttctcgggc cgcgaaatca tggccgagtt tcgccgcgcc acgggactgc
     3301 cgacggcgac caacatgatc gcgaccgatt ggcgccagct tgcgcatgcg ctgcggttgg
     3361 gcgcggtcga catcccgctc gctgacccgc atttctggac catgcaaggc tcggtgcggg
     3421 tggcgcagac ctgccgcgac aa
//
LOCUS       DATLJT010001412         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3336722,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001412 DATLJT010000000
VERSION     DATLJT010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336722"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..214
                     /gene="rpmI"
                     /locus_tag="VM910_32445"
     CDS             14..214
                     /gene="rpmI"
                     /locus_tag="VM910_32445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVI67253.1"
                     /translation="MPKLKTKSGAKKRFKVTATGKVMHAQRGKRHGMIKRTKKQIRQL
                     RGTRVLFKTDGDNIKKYFLPNA"
     gene            298..657
                     /gene="rplT"
                     /locus_tag="VM910_32450"
     CDS             298..657
                     /gene="rplT"
                     /locus_tag="VM910_32450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVI67254.1"
                     /translation="MSRVKRGVTSHAKHKKVYKAAKGYFGRRKNTIRVAKQAVEKAGQ
                     YAFRDRKRKKRTFRALWIQRINAAVRPFGMTYSRFIDGLSKSGIVVDRKVLSDLAISE
                     PAAFQAIAEKAKAALAA"
     gene            772..1878
                     /gene="pheS"
                     /locus_tag="VM910_32455"
     CDS             772..1878
                     /gene="pheS"
                     /locus_tag="VM910_32455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HVI67255.1"
                     /translation="MSDLVTLEQKILGEIAAAGDEGALEAVRVAALGKKGSISALLST
                     LGKMSPDERKTQGAAINLAKDKVTEALAARRDVLRSAALDARLASETIDVTLPVREAP
                     VESGRIHPLSQVMDELTSIFADMGFAIAEGPDIETDDYNFTKLNFPVGHPAREMHDTF
                     FFNSKEDGGSPAETGGTRMLLRTHTSPVQVRTMLSQKPPIRVVCPGRTYRIDSDATHT
                     PQFHQVEGLVIDKGSHLGHLKWILHEFCKAFFEVDHINMRFRPSFFPFTEPSLEADIQ
                     CRRDKGEIRFGEGEDWLEILGCGMVHPNVLRACGIDPDAYQGFAWGMGIDRIAMLKYG
                     MHDLRAFFEADVRWLSHYGFRPLDFPTLAGGLSS"
     gene            1875..4283
                     /gene="pheT"
                     /locus_tag="VM910_32460"
     CDS             1875..4283
                     /gene="pheT"
                     /locus_tag="VM910_32460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVI67256.1"
                     /translation="MKFTLSWLKEHLDTDASVEQLADKLTMIGLEVENIEDKAKLLAP
                     FTIARVISAEQHPNADRLRVCMVDTGDGSAPVQVVCGAPNARAGLVSVFSAPGTYIPG
                     KNITLGVGTIRGVESRGMLCSEAELQLSENHDGIMELPADAPIGKRYAEWAGMGDPTF
                     EINLTPNRQDCAGVHGIARDLFAADMGKFKDPAIKPVKGEFPCPVKVTVDDATLCPGF
                     ALRLVRGVKNGPSPKWLQQRLTSIGLRPINALVDITNFMTYDRARPLHVFDAKKVKGN
                     LVVRRARDGETLLALDGRTYTLDNNVCVIADEHGVESLAGIMGGEASGCDENTTDVLI
                     ESALWNEINIAQTGRKLGISSDARYRFERGVDPAFMVPGLELATRMVMEICGGTPSES
                     VVVGKAHGDDRVIDFPLTEVKRLAGIEVPLPEMRGILTRLGFITAGNGPVMKVAIPSW
                     RSDVEGKADIVEEIVRIVGVDKVPETPFERGEEPRKPVLTPIQLRTRRAKRAAAARGM
                     VEAVTWSFISKQAAELFGGGKSELTLANPIASDLSDMRPSLLPGLIAAAQSNVDRGFA
                     DVALFEVGQIFEGDRPEDQLVAAAGVRRGFASSKGIGRHWSGSAQADAFDVKADALAV
                     LAAAGAPMQALQIVPGGASFLHPGRSGTIQIGPQNILGYFGELHPRTLQALRADGPVV
                     AFEVILDRIPPARQKPTRAKPPLELSLFQPVSRDFAFIVDRAVKAGDIVRTAQNVDKK
                     LISEVTVFDVYEGKGIDEGKKSIAIAVTMQPREKTMTDEEIDAVATKIVAEVTKKTGG
                     TLRG"
     gene            4280..>4679
                     /locus_tag="VM910_32465"
     CDS             4280..>4679
                     /locus_tag="VM910_32465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HVI67257.1"
                     /translation="MSLSGIIPADVSTSAAIAICAIAFVSATARGFSGFGSALIFMPL
                     ASSVASPRLVAALLLIIDFVAAAPLLPNAWTKADRKATGIIAVGALLGVPVGTYLLSR
                     LDPVTTRWIISFFVFALLILLLSGWRYRGKD"
BASE COUNT          881 a         1521 c         1467 g          810 t
ORIGIN      
        1 caaggagagc caaatgccca aactgaagac caagtcgggc gccaaaaagc gcttcaaggt
       61 gactgccacc ggcaaggtga tgcatgccca gcgcggcaag cgccacggca tgatcaagcg
      121 gacgaagaag cagatccgtc aactccgcgg cacccgcgtg ctgttcaaaa ccgacggcga
      181 taacatcaag aaatacttct tgccgaacgc ctgatcggac gcacccccgc tcaaacaaag
      241 ccgagccgcg ttgtttgcgc ggcgaacccg tcacctcaca cgtcaaggat atcagtcatg
      301 tctcgcgtca aacgcggtgt gacgtcccac gccaagcaca agaaagtcta caaggctgcc
      361 aagggctatt tcggccgccg caagaacacg atccgcgtcg ccaagcaggc cgtcgaaaag
      421 gccggccaat acgcgtttcg cgaccgcaag cgcaagaagc gcaccttccg cgcactttgg
      481 atccagcgca tcaacgccgc cgtccgcccg ttcggcatga cctacagccg gttcatcgac
      541 ggcctttcca agtccggcat cgtcgtcgac cgcaaggtgc tgtcggacct cgcgatctcc
      601 gaaccggcgg cgttccaggc gatcgcggag aaggcgaagg ccgcgctcgc agcctgaaac
      661 agggcggcgc gggcgggttc gcccgctcat ttccgaaggc cgcgcgtggc agtgggcgac
      721 ttctttccgg cagaaggcag tcgccgaacg ccacaaggag agatttgacc cgtgtccgac
      781 ctcgtaacgc ttgaacaaaa aatcctgggc gagattgccg ccgccggcga cgagggggcg
      841 ctcgaagccg tccgcgtcgc agcccttggc aaaaagggct cgatttccgc actgctttcc
      901 acgctcggca agatgtcgcc ggacgagcgc aagacgcaag gcgcggcgat caacctcgcc
      961 aaggacaagg tgacggaagc gctcgccgcg cgacgggacg tattgaggtc agccgcgctc
     1021 gacgcccgtc ttgcaagcga gaccatcgac gtcacgctgc ccgtacgcga ggcaccggtg
     1081 gaatccggga gaatccaccc gctcagccag gtgatggacg aactgacctc aatcttcgcc
     1141 gatatgggat ttgccattgc cgagggcccc gatatcgaga ccgacgacta caatttcacc
     1201 aagctgaatt tcccggtcgg ccatccggcg cgcgagatgc acgacacctt cttcttcaat
     1261 tcgaaggagg atggcggctc gccggcagag acaggcggca cgcgcatgct gctgcgcact
     1321 cacacctcgc cggtgcaagt gcgcaccatg ttgagtcaaa agccgccgat ccgcgtcgtc
     1381 tgcccgggcc ggacttaccg catcgattcg gatgcgaccc acacgccgca gttccaccag
     1441 gtcgaaggcc ttgtcatcga caagggttcc catcttggtc acctcaaatg gatcctgcac
     1501 gagttctgca aggcgttctt cgaggtcgac cacatcaaca tgcgcttccg gccgtcgttc
     1561 ttcccgttca ccgagccgtc gctcgaggcc gatatccagt gccgacgcga caagggcgaa
     1621 atccgcttcg gcgaaggcga ggattggttg gagattttgg gctgcggcat ggtgcatccc
     1681 aatgtgctgc gcgcctgcgg catcgacccc gacgcgtacc aggggtttgc ctggggcatg
     1741 ggcatcgacc ggatcgcaat gttgaaatac ggcatgcacg atttgcgcgc gttcttcgag
     1801 gccgacgtgc gctggctctc gcactacggc ttccggccgc tcgatttccc gacgcttgcg
     1861 ggagggctga gctcgtgaag ttcaccctct cctggctgaa ggaacatctc gacaccgacg
     1921 cctcggtcga gcagctcgcc gacaagctga ccatgatcgg gcttgaggtc gagaacatcg
     1981 aggacaaggc gaagcttttg gcgcccttca ccatcgcacg cgtgatttcc gccgaacagc
     2041 atcccaatgc tgaccggctg cgggtctgca tggtcgatac cggcgatggc agcgcgcccg
     2101 tgcaggtcgt ctgcggcgcg ccgaatgcgc gcgcaggtct tgtcagcgtg ttctccgcgc
     2161 ccggtacgta cattcccggc aagaacatca cgcttggcgt cggcaccatc cgaggtgtcg
     2221 agagccgcgg catgctgtgc tcggaggccg agttgcagct ttccgaaaac cacgatggca
     2281 tcatggagct gcctgccgac gcgccgatcg gcaaacgcta tgcagagtgg gcgggaatgg
     2341 gcgatcccac ctttgaaatc aacctcacgc cgaaccggca ggactgcgcc ggcgtgcatg
     2401 gtatcgcgcg cgacctcttc gcagccgaca tgggcaagtt caaggatccc gccatcaagc
     2461 ccgtcaaggg cgagttcccc tgcccggtca aggtaacggt tgacgacgcg acgctgtgcc
     2521 ccggtttcgc gctgcggctg gtgcgcggcg tgaagaacgg tccgtcgccg aaatggctgc
     2581 agcagcgcct gacctcgatc gggctgcggc ccatcaacgc gctggtcgat atcacgaact
     2641 tcatgaccta cgaccgggcg agaccgctgc acgtgttcga tgccaaaaag gtgaagggca
     2701 atctggtcgt gcgccgtgcc cgcgacggcg agacgctgct cgcgctcgac ggccgcacct
     2761 atacgctcga caacaacgtc tgcgtgatcg ccgacgagca tggcgtcgaa tcgcttgccg
     2821 gcatcatggg cggcgaggcg tccggctgcg acgagaacac caccgacgtg ctgattgaat
     2881 ccgcgctgtg gaacgagatc aacatcgcgc agaccgggcg caagctcggc atcagctcgg
     2941 acgcgcgcta tcgcttcgag cgcggcgtcg acccggcttt catggtgccg gggctggagc
     3001 ttgccacgcg catggtcatg gaaatctgcg gtggcacgcc atcggaaagt gtcgtggtcg
     3061 gcaaggccca tggtgacgat cgggtgatcg attttccgct gaccgaggtg aagcggctgg
     3121 ccggcatcga ggtgccgctg ccggagatgc gcggcatcct gacccgtctc ggcttcatta
     3181 cggctggcaa cgggccggtg atgaaggtcg caatcccatc ctggcgcagc gacgtggaag
     3241 gcaaggccga tattgtcgaa gaaatcgtcc gcattgtcgg cgtcgacaag gtgccggaga
     3301 cgccgttcga gcgtggcgaa gagccgcgca aaccggtgct gaccccgata cagctccgta
     3361 ctcgtcgcgc caagcgcgcg gccgctgcgc gcggcatggt cgaggcggta acctggtcgt
     3421 tcatctcgaa acaggcggcc gaattgttcg gcggcggcaa gagcgagctc acgctcgcca
     3481 atccgattgc ctccgatctt tccgacatgc ggccgagcct cctgcccgga ctgatcgcgg
     3541 ccgcacagtc gaatgtcgac cgtggttttg ccgatgtggc cctgttcgag gtcggccaga
     3601 tcttcgaggg cgatcggccg gaggatcaat tggtcgccgc cgcgggcgtc cgccgcggct
     3661 ttgcctcgtc gaagggaatc ggacggcact ggtcgggttc ggcacaagcc gatgcgttcg
     3721 acgtcaaggc cgatgcactc gcggtgcttg ccgccgccgg cgcgccgatg caggcgctgc
     3781 aaatcgtacc gggcggagcc agttttctgc atccaggacg ttccggcacg atccagatcg
     3841 gtccacaaaa tattctgggc tactttggcg agttgcatcc ccgcacgctc caggctctgc
     3901 gcgccgacgg tccggtggtt gcgttcgagg tcattctcga tcgcatccct ccggccaggc
     3961 agaagccgac ccgcgccaag ccgccgctcg agctctcgct gttccagccg gtgtcgcgcg
     4021 acttcgcctt catcgtcgac cgcgccgtga aagccggcga catcgtgcgc acggcacaaa
     4081 acgtggataa gaagctgatc tccgaggtca cggtgttcga cgtttatgag ggtaaaggca
     4141 tcgacgaggg caagaagtcg atcgcaatcg ccgtgaccat gcagccgcgc gaaaaaacca
     4201 tgaccgacga agagatcgat gcggtggcga caaagatcgt ggccgaggtg acgaaaaaga
     4261 ccggcggcac gttgcgcgga tgagtctgtc gggcatcatc ccggccgacg tcagcaccag
     4321 cgccgcgatc gcgatctgcg cgatcgcctt cgtctccgcc accgcgcgcg gattctccgg
     4381 cttcggttcg gcgctcattt tcatgccgct cgcaagcagc gtggcctccc cgcgcctcgt
     4441 cgccgcgctg ctcctgatca tcgacttcgt tgccgccgct ccgcttctcc cgaatgcgtg
     4501 gacgaaggcg gaccgtaagg ccaccgggat catcgcggtc ggcgcgctgc tcggagtccc
     4561 cgtcgggacc tacctcctga gccggctcga tcccgtgacc acgcgctgga tcatctcctt
     4621 tttcgtgttt gcgctcctga tcctgctgct gtccggctgg cgctatcgcg gcaaggacc
//
LOCUS       DATLJT010001413         1711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_119710,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001413 DATLJT010000000
VERSION     DATLJT010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119710"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..495)
                     /locus_tag="VM910_32470"
     CDS             complement(172..495)
                     /locus_tag="VM910_32470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67258.1"
                     /translation="MQNTAAMRVIRLHELPAFLGVRRSQINEAVRAGWLHPFTPIPGG
                     RGKVVTEAEVIALQQQAMAEAAAAAEAAKAPAAEPAPIEVKPATPAPVGRKRKPATAE
                     RALTA"
     gene            complement(616..1539)
                     /locus_tag="VM910_32475"
     CDS             complement(616..1539)
                     /locus_tag="VM910_32475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67259.1"
                     /translation="MKAPDKKSGKSTTSTAAPPARAASFTIEAQAEGAVEKALDKKSD
                     ESTSTAAPPAEAQAEGAVEKAPDKKRSTVGLTVRLSSRWLGSAPADKTFAYSEADWQS
                     IKASPGRVGIDADAVTVGDHWWAQPDPAAALAAALQRPLREQLQELAAAYRGLAAYCK
                     QGEALTPLQEAGEIQEKLKKLEAARAALDSRLLDFIPRTPDAREALTVVIAHAKRHVN
                     LLRAEGSRSSLNACKVHFEYWDKLTLLWQAITANQNRRPRGRGLNSFLLACSKSVFPD
                     ETSKSNIKNFRQNVRRKGVNDRRKSGVVLPF"
BASE COUNT          273 a          555 c          523 g          360 t
ORIGIN      
        1 caatgaaccg aggaaggtcc gaccgtcgta aaccgaaacc tcgcctgtgg gcgggccttg
       61 cgaagatctg cgaggtgtgc aactgaccat gacgcttgat ggtgccgttt tgggttgccg
      121 cccgattcgg agaagcagcg cgccgaccct ggcaggggtc ggcgcctgcg cttaagcggt
      181 cagcgcgcgc tcggcggtcg cgggcttgcg cttgcggccg accggtgcgg gcgttgcggg
      241 cttgacctcg atgggggcag gctcggccgc cggggccttc gctgcctcgg ctgcggcagc
      301 cgcctcggcc atcgcctgtt gctgtaaggc gatgacctcg gcctccgtca caacctttcc
      361 gcggccgccg gggattggtg tgaatggatg caaccagccc gcccgcaccg cctcgttgat
      421 ttgagacctt cgaacgccga gaaatgctgg caactcgtga agccgtatca ctcgcatcgc
      481 tgccgtattc tgcacgatgg attctcccaa aaactttgtc gtcgccggag ggaatgccca
      541 tccggctgtg gccggacgga tgtgtccggc gccctaaaaa ttatatggtc ggattcgcga
      601 tcgcgatgca cgcattcaaa agggtaaaac gacgccactt tttcgccggt cgtttacacc
      661 ttttcgacgg acgttctgac ggaagttctt gatgttgctt ttggatgtct cgtcggggaa
      721 taccgacttc gagcacgcga gcaggaatga attcaagcct cggccgcgcg gtcgccggtt
      781 ttgattggcg gtgatcgctt gccacaacag cgttagcttg tcccagtatt cgaaatgcac
      841 cttgcaagcg ttgaggctgc tgcggctgcc ctcagccctc agcaggttaa catgccgctt
      901 cgcgtgagcg atgaccaccg ttaacgcctc gcgggcgtcc ggcgtccgcg ggatgaaatc
      961 cagcagacgc gaatcgagcg cggcgcgagc ggcctccagc ttcttcagct tctcctgtat
     1021 ctcgccggcc tcttgtagcg gcgtcagcgc ttccccttgc ttgcaatatg ccgcaagccc
     1081 tcgataggca gcagcaagct cttgcaattg ctcgcgcagc ggtcgctgta acgccgccgc
     1141 cagcgcagcc gcaggatccg gctgcgccca ccagtgatcg ccgaccgtca ccgcgtcggc
     1201 atcgatgccg acacgaccgg ggctggcctt gatgctctgc caatcggcct cgctgtaggc
     1261 aaatgtttta tcggccggcg cacttccgag ccatctcgaa ctgagcctca ctgttaaacc
     1321 aactgtgctt cttttcttgt cgggcgcttt ctcgaccgcg ccttccgcct gagcctcagc
     1381 aggcggcgcg gccgtgcttg tgctctcgtc gcttttcttg tcgagcgctt tctcgaccgc
     1441 gccttccgcc tgagcctcaa tggtaaaaga tgccgcacgc gcaggcggcg cggcggtgct
     1501 tgttgtgctc ttgccgcttt tcttgtcggg cgctttcatg gacgcgcccg ccgaatcggc
     1561 accacatcgg cggcgtcgat cggtgggccg tagcagaagt ccgcccacat cgacatcaac
     1621 ttggcgcgct cggcgagcag atcggatcgc gcatacgacc tcgccgactt gctgccgacg
     1681 acgtgcgcca gcgtggcctc gcacacctcg a
//
LOCUS       DATLJT010001414         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1271004,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001414 DATLJT010000000
VERSION     DATLJT010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271004"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VM910_32480"
     CDS             <1..1557
                     /locus_tag="VM910_32480"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67260.1"
                     /translation="ASPVKAVRCAIEMQHGMVDRNADVPREQRIEFRIGINLGDVVVE
                     DGDIYGDGVNVAARLENIAEPGAVYISRAVRDFVDDHAEMTLEDLGEKSLKNIAKPVR
                     VFRIAAPDQAVAVPAPVGSPALPHKPSIAVLPFANMSGDAEQEYFSDGMTEDLITDLS
                     KISALFVIARNSSFAYKGRSVKVQEIGHDLGVRFVLEGSIRKAGNRVRITAQLIDAGS
                     GGHLWAERFDRDLTDIFATQDEVVEKIVGALAVKLTQGEERRLRRRGTSNVEAYETWL
                     RARELLSRSTRDGVAQARAKHLRAIELDPSFAEPHAGLSLAAISDYVSDWAADPAQAL
                     DEAERWARRALELDDQEPVSHMALGNVLLWRRNLEGALAEFRRMIELDPNFAQGHSAT
                     GLALMYAGRPAEALEAFAIAKRLDPHHASIVLHFRAQTHFSLGQYETAVKQLLERIAR
                     TPATDSSRMMLASCYGHLGRADDARAAWAELLKVNPDFSLAQRERVLPYKNAADFQRI
                     VEGLAKAGLP"
     gene            1713..>2069
                     /locus_tag="VM910_32485"
     CDS             1713..>2069
                     /locus_tag="VM910_32485"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HVI67261.1"
                     /translation="MKICYAVVVAALAGAGIGGLTVERLHAQAKPPAYYIAEIDVTNE
                     DLYNKEWAPKADATIKAAGGIHLVRGTNIERIEGAAPKRLLITKWDNIDKLTAWRESE
                     AYMRTLPILDKAVKSVR"
BASE COUNT          403 a          632 c          692 g          342 t
ORIGIN      
        1 gccagcccgg tcaaagcggt gcgctgcgcg atcgaaatgc aacacggcat ggtcgatcgc
       61 aacgccgatg tcccccgcga gcagcggatc gaattccgca tcggaatcaa tctcggcgac
      121 gtggtcgtgg aggacgggga catctacggc gatggggtga acgtggccgc gcgtctggaa
      181 aacatcgccg agccgggcgc ggtctacatt tcgcgcgccg ttcgcgattt cgtcgacgac
      241 cacgccgaga tgacattgga ggatttgggc gaaaagtcgc tcaagaacat cgccaagccg
      301 gtgcgggttt tccgcatagc cgcgccggat caagcggtgg ctgtgcctgc gccggtggga
      361 tcgccggcgc tgccacacaa gccgtcgatc gccgtgttgc cgttcgcgaa catgagcggc
      421 gacgccgagc aggaatattt cagcgacggc atgaccgaag acctgatcac cgatctctcc
      481 aagatctcgg ccctgttcgt catcgcgcgc aactcctcct tcgcctacaa gggcaggtcg
      541 gtcaaagtgc aggagatcgg gcacgacttg ggcgtacgct tcgtgctcga gggcagcatc
      601 cgcaaggccg gcaatcgtgt gcgcatcacg gcgcagctca tcgatgccgg cagcggcggg
      661 catctgtggg cggaacggtt cgatcgcgat ctcaccgaca tcttcgcgac ccaggacgag
      721 gtggtggaga agatcgtcgg cgcgctcgcg gttaagctga cgcaaggcga ggagcgacgg
      781 ctgcgccggc gcggcacctc caatgtggag gcatacgaga cctggctgcg cgcgcgcgaa
      841 ttgctgagcc gcagcacgcg ggacggggtc gcgcaagccc gggcgaagca cctgagggcg
      901 atcgagctcg atccgagttt cgccgagccg catgcgggac tgtcgctggc cgcgatttcc
      961 gactacgtca gcgactgggc ggccgatcct gcgcaggcgc tcgatgaggc ggagcgatgg
     1021 gcgcgccggg cgctcgaatt ggacgatcag gagccggtga gccacatggc gctcggcaac
     1081 gtgctgctgt ggcggcgcaa tctcgagggc gcgctggcgg agtttcgccg catgatcgag
     1141 ctcgatccca actttgccca gggccattcc gcgacgggac tggcactgat gtatgccggc
     1201 cgcccagccg aggcgctcga ggcgttcgcg atcgcgaagc ggctcgatcc gcaccatgcg
     1261 tccatcgtgc tgcacttccg ggcgcagacg catttcagcc tgggacaata tgaaaccgcc
     1321 gtgaagcaac tcctcgagcg catcgcgcgc acccccgcca ccgactccag ccgcatgatg
     1381 ctggcgtcct gctacggcca tctcgggcgc gcggacgacg cacgcgcggc gtgggcggag
     1441 cttctcaaag tgaatccgga cttctcgctg gcgcagcgcg aacgcgtcct gccgtacaag
     1501 aacgccgctg attttcagcg tatcgtcgaa ggcctggcga aggcgggatt gccgtaggcg
     1561 tcccgagcga ggaaggtcgt cggacgagcg tgaccgtcgc cgagatgata gaatgagtgc
     1621 gggggaattt attgctgccc aatcgcccct cgaaaaagct ttgaatgagg tttcgcgatc
     1681 aaaggtctag cccacagcta aggattgatc tcatgaaaat atgctacgcg gtggtagttg
     1741 ccgcgctcgc gggtgccggc atcggtggcc tgacggtcga gcgccttcac gcgcaagcca
     1801 agccgccggc gtattatatc gccgaaatcg atgtgaccaa cgaggacctt tacaacaagg
     1861 agtgggcccc caaagccgac gccacaatta aagccgcagg cggcatccat ctcgtgcgcg
     1921 gcacgaacat tgaacggatc gaaggagctg cgccaaagcg gctgttgatc accaagtggg
     1981 acaacatcga caagctgacg gcttggcgtg aatccgaggc ctatatgcgg acgctgccga
     2041 tcctcgataa ggcggtcaaa tcggtgcgc
//
LOCUS       DATLJT010001415         4787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1271130,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001415 DATLJT010000000
VERSION     DATLJT010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271130"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VM910_32490"
     CDS             complement(<1..717)
                     /locus_tag="VM910_32490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67262.1"
                     /translation="MSARFEPIVGRYMHLDLFGKPHRLYVEEAGQGTPLLCLHTAGSD
                     GRQYRALMNEKRITSRFRVIAFDMPWHGKSSPPAGWHNEEYQLTSAQYTTMVLSVMTA
                     LELDRPVLIGCSIGGRIALHLALEHPQAFRAIIGLQAGAHIDPYYDLNFLHRPDIHGG
                     EVCAAIVSGLVGPDAPEKERWETLWHYMQGGPGVFKGDLYFYKIDGDIRSRVADIDTG
                     RCPLFLLSGEYDYSCTPEETL"
     gene            complement(714..1082)
                     /locus_tag="VM910_32495"
     CDS             complement(714..1082)
                     /locus_tag="VM910_32495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67263.1"
                     /translation="MLEALKERVNADAALVRRGRYLTTTFLIEIGEMPWLISIFEGRI
                     VSVARGPFVMPSSSFELRASEAEWQKFFSAHPPPGSNDLMALIRRRVLKAEGDLKVFM
                     AHLRYFKEALAKLRPTGGLA"
     gene            1458..1712
                     /locus_tag="VM910_32500"
     CDS             1458..1712
                     /locus_tag="VM910_32500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67264.1"
                     /translation="MAYALFCNDAKLSKAYPTEADVWKLARQSGLVVDAVSEHGEPVP
                     QPILDNDYEIRVCLPDPHEDPVQNRADAEREAQHELQFTS"
     gene            complement(1767..2003)
                     /locus_tag="VM910_32505"
     CDS             complement(1767..2003)
                     /locus_tag="VM910_32505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67265.1"
                     /translation="MRIFASVLILCAVAIPSSAQTVLKSEPLVLAPYEVAFVQDATCP
                     VGKVLKVTGAIRGLHRKRACVALSTRASLAIATP"
     gene            complement(2149..2856)
                     /locus_tag="VM910_32510"
     CDS             complement(2149..2856)
                     /locus_tag="VM910_32510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HVI67266.1"
                     /translation="MKKLLLSAAVLAGTVLPASAADMPPRTYTAPAYTAPALIYNWTG
                     FYIGGHLGGAFGDDNGLLGSGGRFMGGVQGGFDYQFAPNWVAGVEAQYSWLNSNTTGV
                     LFPANTLVISRNDQIGSVTGRFGYTWGPALLYAKGGFAWRDNPNIGVTVGGTPVAFAT
                     TGGNNNGWTVGAGLEYMFAPNWSAKAEYQYYNFGTTTFATGPAPIVGSRFRDDDQTVK
                     VGVNYRFGWGGPVQPRY"
     gene            complement(3104..3463)
                     /locus_tag="VM910_32515"
     CDS             complement(3104..3463)
                     /locus_tag="VM910_32515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67267.1"
                     /translation="MDSDDTRATSFMEDSSGNQPFPSNYRTEVLAFMHTYLNNPVGVR
                     EAAMADPVQRTVNGRARFVSCLRFAEPHSDGTYRDARERAILFVNGRLDRMLQNASEV
                     CAGAVYAPFPELEKMTR"
     gene            complement(3719..4042)
                     /locus_tag="VM910_32520"
     CDS             complement(3719..4042)
                     /locus_tag="VM910_32520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67268.1"
                     /translation="MLKTISAALLAVSVLAAPALAATSGKTAQAPVSKTATDKATTAR
                     NATARNAIGKSNSVKPNVMNANAKMGKHHAKHARHHRHHSNVAAIKAKSKVGAKPVSS
                     TTKRG"
     gene            4372..>4787
                     /locus_tag="VM910_32525"
     CDS             4372..>4787
                     /locus_tag="VM910_32525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI67269.1"
                     /translation="MNKFSIRLLALAMFSMSLIAAPLATAYAAGDENPSPPASGKKDK
                     KKGTQLIPSGQSEDYAKFANGYHAAYSTVYDRQDYATAIDQLKTLGHDDNAAVANLIG
                     YSYRKLGDYKLSQVWYERALKADPNHVKTWQYYGLW"
BASE COUNT          924 a         1444 c         1489 g          930 t
ORIGIN      
        1 cagcgtctcc tccggggtgc aggaataatc gtactcgcct gacagcagaa acagcggaca
       61 gcgtccggtg tcgatgtcgg cgacgcggct gcggatatcg ccgtcgatct tgtagaaata
      121 gagatcgccc ttgaagacgc cgggaccacc ctgcatgtag tgccacagcg tttcccagcg
      181 ctccttttcc ggtgcatcag gcccgaccag cccggagacg atggcggcac aaacctcgcc
      241 gccatggata tccggccgat gcaggaaatt caggtcgtaa tagggatcga tatgcgcgcc
      301 ggcctgcagc ccgatgatgg cgcggaacgc ttggggatgt tcgagcgcca gatgcagcgc
      361 gatgcgcccg ccgatcgaac agccgatcag gaccgggcga tccagttcga gggccgtcat
      421 cacggacagc accatggtgg tgtattgcgc cgacgtgagc tgatactcct cattgtgcca
      481 gcctgccggc ggcgaggact tgccgtgcca gggcatgtcg aaggcgatca cccgaaagcg
      541 cgaagtgatg cgcttttcgt tcatcagcgc gcgatactgg cgtccgtcgc tgcccgcggt
      601 gtgcagacat agcagcggcg taccctggcc ggcttcctcg acatagaggc ggtgcggctt
      661 gccgaacagg tcgagatgca tgtagcggcc gacgatcggc tcaaatctcg cgctcatgcc
      721 aaacctcctg ttggacgaag ctttgccagc gcctccttga agtagcgcag atgcgccatg
      781 aagaccttga gatcgccctc ggccttcaac acgcgacgcc tgatcagcgc catcaggtcg
      841 ttggaaccgg gcggcggatg cgcgctgaag aacttctgcc attcggcctc gctggcgcgc
      901 agctcgaacg acgaggacgg catcacgaag ggaccccgcg caaccgagac gatgcgccct
      961 tcgaagatcg agatcagcca cggcatctcg ccgatttcga tgaggaacgt cgtggtcaga
     1021 taccggccgc ggcgaaccag cgcggcgtcc gcattgaccc gctccttcaa cgcctcgagc
     1081 atcgcgaagc ctccctcacc tgccggacct tggcggcctg tttgggtgtc atgatggaga
     1141 gatcggacgg ggcgtcaacc tgctaaagga aaccccatac cagcacgaca ttggccgtgc
     1201 aggcgatcag aagccccacg gcaagtcgaa actgcacccg ctcgctggct gtatggacca
     1261 tcgttcccat ggttgaaagc atccggcgat tctgggcggc gagtctcggg gattcttttt
     1321 tacggaattc atatcgcgtt tacgactcag gcacctcgca aaaggtccgt tccggtccga
     1381 ttctgcccgc cgcgattcca aggccgcaac cgagacccct gcttgcgcgt taagtcagtt
     1441 ctgacggagc aacgccgatg gcttacgccc tgttttgcaa cgatgccaag ctgagcaagg
     1501 cctacccgac cgaggccgac gtgtggaaac tcgcccggca aagcgggctc gtcgtcgacg
     1561 ccgtttccga gcatggcgaa ccggtgccgc agccgatcct cgacaatgac tatgagatca
     1621 gggtttgcct gccggatccg catgaggatc ccgtgcaaaa ccgcgccgat gccgaacgcg
     1681 aggctcagca cgagttgcag ttcacgtcgt gattcaaaaa ccggcctgat gcgctggtct
     1741 ggccgacttc gaggcgatgg caactctcaa ggagtcgcga ttgcaagcga agctcgggtg
     1801 gacagcgcga cgcatgctct cttgcgatgc aatcctctga tcgcgcccgt caccttgagc
     1861 accttgccga caggacaagt ggcgtcctgc acgaaagcaa cttcgtacgg cgcgagcacc
     1921 aggggttcgg atttgagaac ggtttgagca gagctaggga tcgccaccgc gcacaaaatc
     1981 agcaccgacg cgaaaatacg catgttcatt gtccacagcc cggcagctaa taatagccga
     2041 tctgcgtgac aagtttcgcg cgcggatcaa aatatttttt gcgctgcgcc cgacaacgaa
     2101 aaggccggca agctgccggc cctttcgctt tcagtttcct gatcggcgtc agtatctcgg
     2161 ttgtaccggc ccgccccagc caaagcggta gttcacaccg accttgacgg tctggtcgtc
     2221 gtcgcggaag cgcgatccga cgatcggcgc cggtccggtg gcgaaggtgg tggtgccgaa
     2281 attgtagtac tggtactcgg ccttggccga ccagttgggc gcgaacatgt attcaaggcc
     2341 ggcaccgacg gtccagccgt tgttgttgcc gcctgtggtg gcaaaggcaa ccggcgtgcc
     2401 gcctacggtc accccgatgt tgggattgtc gcgccaggca aacccgccct tggcgtagag
     2461 cagtgccggg ccccaagtgt aaccgaaccg gccggtcacg gacccgatct gatcgttcct
     2521 gctgatgacc agcgtgttgg ccgggaacag gacaccggtg gtgttgctgt tcagccagct
     2581 atactgggcc tcgacgccgg ctacccaatt gggcgcaaac tggtaatcga aaccgccctg
     2641 cacgccgccc atgaagcggc cgccgctgcc gagcagtccg ttgtcgtccc cgaaagcacc
     2701 gccgagatgg ccgccgatgt aaaagccggt ccagttgtag atcagggccg gcgccgtata
     2761 ggcgggcgcc gtataggtgc gcggcggcat gtcggcggcg gaagcgggta acaccgtgcc
     2821 ggccaggacg gctgcgctaa gcaacagttt cttcattgga attccccatt acgcggtcgc
     2881 ggcatcaacc aacaaattga cctcaatttg gttgcctcgc ggcaatgccg catctgtgat
     2941 gtcgcgaatg tgacgatgtt tttcggaccg tggtcctgct tgccctttgt tttgtgcccc
     3001 gcctatccct ttgggatttg agggcttttc acccgttgtt tggtcggggc ggcctgggca
     3061 ttggttcaag gctcgccaaa aagtgatcgc aggcgtcggc cggttaacgg gtcatctttt
     3121 ccagttcggg gaacggcgcg tagacagcgc cggcgcagac ttcgctcgca ttttgcagca
     3181 tgcggtcgag ccggccattg acgaacagga tcgcccgctc gcgcgcgtcc cgataggtgc
     3241 catcggagtg cggctcggca aaacgcaggc agctcacgaa gcgcgcgcgc ccattgacgg
     3301 tccgctgcac gggatcggcc atggcggcct cgcgcacgcc gaccggattg ttgagatagg
     3361 tgtgcatgaa cgccagcacc tccgtgcggt aattgctcgg aaatggctgg ttgccgctgc
     3421 tatcttccat gaaggaggtt gccctggtgt cgtcgctgtc catgcaggcg gacaagacaa
     3481 gcggcaacag caagatcgcc aggcacttcg ctttggtccc caaattcgac tcgtctcgta
     3541 aggtcgtcgc cgggggcggt tctagcccct cctcgcgcgg aagggaaagt gcatttctgc
     3601 aacgtttcga gagcggacac cggcccgcag caacaggggg caccattgcg tgcgagccgg
     3661 ccttccgccg acggcgcggt ttcggggcaa tgtggaacga gccgcgccga aggagttatc
     3721 agccgcgctt ggtcgtggac gaaacgggct tggcgccgac cttcgacttc gccttgatcg
     3781 cggccacgtt gctgtggtga cgatgatggc gcgcgtgctt cgcgtggtgc ttgcccattt
     3841 tggcgttggc gttcatcacg ttgggcttga ccgaattgct cttgccgatc gcattcctgg
     3901 cggtcgcatt cctggcggtc gtggccttgt cggttgcggt cttgctcacg ggcgcttggg
     3961 cagtcttgcc ggaagtcgcg gcgagcgccg gcgcggcgag aacggaaacg gcaagcaaag
     4021 ctgcggaaat cgtcttgagc atttgtgtct cctcttcaaa tgatcttgaa gtggccgtcc
     4081 gaatgatcgg tcacgtcgat cgtgctcgcg acgctaatcg ttccgcactg aaccggcggt
     4141 gaaacgctgt gccagacagc gttcatgtcc atgacttgtt gttcattccg gaaaatgccg
     4201 gcagaaggcg ctccagcatt gtgtggcgac aataagcacg catcgtgacg gcatgccggg
     4261 aatgttttgg ggtcgaaggt gttctagtcg aacggatcag gtgtaggatc acgcatggcg
     4321 cgcatggttg ccgcgtgggc ctgcgcccta ctgcaatcgg gagagaaatg catgaacaag
     4381 ttttcgatca ggctgctggc gctggccatg ttctcgatgt cgttgatcgc cgcacctctg
     4441 gccacggcct atgcggcggg tgacgagaat ccgtcaccgc ctgcctccgg caagaaggac
     4501 aagaagaaag gtactcagct aatcccgagc gggcagagcg aggactacgc aaaatttgcg
     4561 aacggctatc atgcggccta ttccaccgtc tacgaccgcc aggactatgc cacggcgatc
     4621 gatcagctta agactctcgg acacgacgac aatgcagcgg tcgccaatct gatcggctat
     4681 tcctatcgca aactcggcga ctacaagctg tcgcaagtct ggtacgaacg cgcgctgaag
     4741 gccgacccga accacgtcaa gacctggcag tattacggcc tctggca
//
LOCUS       DATLJT010001416         9164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1039444,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001416 DATLJT010000000
VERSION     DATLJT010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039444"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VM910_32530"
     CDS             <1..323
                     /locus_tag="VM910_32530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HVI67270.1"
                     /translation="NDAPALAAAYASMSPVSATHLSQSVADAVFLGERLAPVGLAIAI
                     SRKALLLMRQNLWLAVVYNALAVPIAIAGVVTPLIAAAAMSGSSLLVMLNALRARFHQ
                     KETF"
     gene            323..484
                     /gene="ccoS"
                     /locus_tag="VM910_32535"
     CDS             323..484
                     /gene="ccoS"
                     /locus_tag="VM910_32535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HVI67271.1"
                     /translation="MEVLVFLVPLAIMLGAIGLMGFLWSLKNGQYDDLDGAAWRAIAD
                     DEPAESSNG"
     gene            646..1317
                     /locus_tag="VM910_32540"
     CDS             646..1317
                     /locus_tag="VM910_32540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI67272.1"
                     /translation="MLSVATNSSMFVRPPMPTRTAGLPDGPLALMGAPMRFARNAEVY
                     GEDEPAEYLYQVISGAVRTYRMLDDGRRQVVAFYLPGDIFGVEAGDVHLSSAEAVSEA
                     QVLVAKRSAVLARAEREKQLSRQLWDLMVRELQRVQQHSFVLIKSADERVAGFLLEMA
                     GRSSGGASIELPMSRQDIADYLGLTIETVSRTFTQFVQSGLISLETSRRIQFRDRAAL
                     NRLNA"
     gene            1553..2809
                     /locus_tag="VM910_32545"
     CDS             1553..2809
                     /locus_tag="VM910_32545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HVI67273.1"
                     /translation="MTAPRRDLAASIDPAKLDRLAEVAVKVGLRLREGQDLLLTAPAA
                     ALPLVRKVAEHAYKAGAGVVTPILSDEEFTLARYRFGHEDSFDRAADWLYEGMAKAFA
                     QNTARLAIVGENPMLLSGEDPSKVARASKANSKAYQPALEKIVNFDTNWNIIAYPSPS
                     WAKLVFPDVAEEIAVAKLADAIFSASRVDEDGAIAAWDRHNAKLRERTEWLNGQRFSA
                     LHYSAPGTNLTIGLADGHEWEGGASTARNGITCNANIPTEEVFTTPHCRRVNGRVVSS
                     KPLSYQGTLIDNIQVRFEGGRIVEARASRGEEVLNKVLDTDDGARRLGEVALVPHSSP
                     ISKSGLLFFNTLFDENAASHIALGQCYSKCFIDGAKLTPQQIAAQGGNQSLIHIDWMI
                     GSDKTDIDGVHADGRRVPVFRKGEWA"
     gene            complement(2917..3774)
                     /locus_tag="VM910_32550"
     CDS             complement(2917..3774)
                     /locus_tag="VM910_32550"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67274.1"
                     /translation="MSIVLRAGTPADAAVCGPICFRAFKSINDTHNFPPDFPSPEIAT
                     GVLTMLLAHGGFYSVVAEKDGKIVGSNFLDERGPIAGVGPITVDPSIQNQTIGKSLML
                     AVLDRARARKAAGVRLLQSAFHNRSLCLYTKLGFDSRETVSKIDGAALNLKLPGYEVR
                     PARTADVAACNALCRRVHGHDRGGELEDAIKAGTGRVVEHLGEITAYASDIAFFAHAV
                     ADNNNGLKALIGAAQMFPGGGFLLPSRNSELFRWCLAHRLRLVHQMTLMTIGLYDEPQ
                     GAYLPSVLY"
     gene            3955..4578
                     /locus_tag="VM910_32555"
     CDS             3955..4578
                     /locus_tag="VM910_32555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67275.1"
                     /translation="MHIFSSRLILAVVVAALYPGGRIAHAQAGPMSYWTPGWPVGFSG
                     DFATGQGANTYANFPAFNGSDAGGFGYARYNFQNGWFVGSERGGMGLSTSGFNQIGAF
                     GNSLSYEGLQFGYNFQKSTGLPLTIYAGFDTLKYTTGIGSPFAPFDSMSGTLPGYSAR
                     AGVEFQPTSNVSLSLGFGYTQQSGRVDSDINSLSLPGAYQFPYAGRR"
     gene            4730..5182
                     /locus_tag="VM910_32560"
     CDS             4730..5182
                     /locus_tag="VM910_32560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67276.1"
                     /translation="MSRALIEFGNTEVELTPRPIERSWIIEGDPQASSCVLSQSADGL
                     ATTMVWHCTAGKFNWYYDFDETVLILDGGIVIESEGMKPTHYGPGDVIFFKDGAHAIW
                     HVEDHVRKLAFCRKTQPVWLGFAVRVFLKLKKIMMGPAAREPASLMAR"
     gene            complement(5231..6286)
                     /locus_tag="VM910_32565"
     CDS             complement(5231..6286)
                     /locus_tag="VM910_32565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67277.1"
                     /translation="MVVSTAPVSASGLYADPREDWLKQHTEEIIDPARPIVDPHHHLW
                     DRGGLRYLIEEITDDIASGHNVVATVYVEARSMYRAFGPEAFRPVGEVEFANGAAAMA
                     ASGAYGKALVCAGIVGHVNLLAGDGAKAVLEAEIAAGGGRFRGIRHSSAWDADKEAAG
                     IYATRPKGLLLDSTFRKGFACLAPLGLSFDAWLFHPQLGEFADLARAFPDTRICLDHC
                     GGIIGIASYANRRDEIFKTWKASIREVAKCENVVVKLGGLAMRLPGFDFDRRPKPPTS
                     EEAATAWKPYIETCIEAFGPQRCMFESNFPPDKGQCSYQVIFNAFKRIAAACSESDKN
                     ALFSRTAMEFYRLKLDA"
     gene            complement(6329..7144)
                     /locus_tag="VM910_32570"
     CDS             complement(6329..7144)
                     /locus_tag="VM910_32570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI67278.1"
                     /translation="MLRSVSLVFAFVGLLIAPAAAENAPRSECLAISKAPARATPVSL
                     RRAAANTDEVAITYGGHSTYFIDTPGGVRVATDYSGAYQTGRLPDVVTMNRAHSTHYT
                     LFPDPRIPHVLHGWGDDGDPAKISERIGDVFIRNVTTDIRRFFGDYSGVDMIKDGNSI
                     FIFEVAGLCIGHLGHLHHQLDDTHFAAIGRLDIVMVPIDGAYTMSLGGISDITKKLRA
                     SVVLPMHRFATPLEEFMERIGKEFEIDRRSERTLRISRETLPTTPTVIILDGV"
     gene            7252..8178
                     /locus_tag="VM910_32575"
     CDS             7252..8178
                     /locus_tag="VM910_32575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67279.1"
                     /translation="MMSRAASSQRLEPLRHVEAGVLKIAYFEAGPADGPVVLLLHGFP
                     YDIHSYVDLAPMLAERGCRVIVPYLRGYGPTRFLSAATPRSGEQAAIGADLMALMDAL
                     SVPRAVFAGYDWGGRAACIGAALWPERCFGLVSVNSYQIQDIANALSPARPEREVPLW
                     YQWYFQIERGRAGLAADRRGITRILWRQWSPNWQFDEAMLERTSVAHDNSDYVDVVIH
                     SYRHRYGLADGDPRYADIQRRLAALPPITVPAITLDGDADGVVPATDGSASAAKFSGP
                     RVHRVIPRAGHNLPQEAPEEFAVAVMELVKRS"
     gene            complement(8186..>9164)
                     /locus_tag="VM910_32580"
     CDS             complement(8186..>9164)
                     /locus_tag="VM910_32580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HVI67280.1"
                     /translation="RTRLIAQILAAIIGAGFVIALQIAAILSYGTLSRFAVLTSDAAA
                     ALAPDADSILWWPARAVLGDFVILLLLLSAGLVMLGLVMIVLAPRFVGTAANASAQSA
                     SARQGRVAKAFRGGSRQQALRRKEFLLLQRDPWLMSQTLMQLLYLVPPALFLWRSFSD
                     QSTAIVLITPVIVMAAGQLAGGLAWLTISGEDAADLVATAPLPPSRVTRAKVEVVLIV
                     IGVIFAPLVAGLAFVAPLQAVVTMAGVVVAATSATAIQLWFRVQAKRSQFRRRQTSSR
                     LATFAEAFSSIGWAATAALALAIPAAAMVSGLMTVVIVAATWKLSPRRD"
BASE COUNT         1689 a         2835 c         2942 g         1698 t
ORIGIN      
        1 tgaacgatgc gcccgcgctt gccgcagcct atgcctcgat gtcgccggtt tcggcaaccc
       61 atttgagcca gtccgtggcg gatgccgtgt ttctcggcga gcggctcgcg ccggtgggtc
      121 ttgcgatcgc catctcgcgc aaggcgctgc ttttaatgcg gcagaatctg tggcttgcgg
      181 tggtctacaa cgcgcttgcg gtgccgatcg cgatcgcggg cgtggtgacg cccttgatcg
      241 cggccgctgc gatgtccggc tcctcgctgc ttgtcatgct caacgcgctg cgcgcgcgat
      301 ttcaccagaa ggagactttc tgatggaagt tctggtgttt ctcgttccgc tcgccatcat
      361 gctcggcgcc atcggcctga tggggttcct ctggtcgctg aagaacggcc aatatgacga
      421 tctcgacggt gcggcgtggc gcgccatcgc cgacgacgag cccgccgaat catcgaacgg
      481 ttaggcagcc ggaagcttcg acctggctca acgggcggcg gcccgcaaag gcccgtgaaa
      541 ttacgggctc cttccggatt tatacttagg ggaacgaccc gaatttcgaa atttctggga
      601 acaggcagac ttgcggttac agacgatcga aaccggagtt tcgccatgct ctcagtcgcc
      661 accaattcct cgatgtttgt ccgtccgccg atgccgactc gcacggccgg actgcccgac
      721 ggcccacttg ctttgatggg cgccccgatg cgatttgccc gtaacgccga agtctatggc
      781 gaggatgagc cggctgagta tctctaccag gtcatttccg gcgcggtgcg aacctaccgt
      841 atgctggatg acggccggcg tcaggtcgtc gccttctatc tgcccggtga catcttcggc
      901 gtcgaggcgg gtgacgtgca tctgtcctcg gcagaagccg tcagcgaagc ccaggtgctg
      961 gtggccaagc gaagcgccgt gttggcccgt gccgagcgcg aaaagcagct ttccaggcag
     1021 ctttgggacc tgatggttcg cgagttgcag cgcgtgcagc agcattcctt cgtcctcatc
     1081 aagagtgccg acgaacgggt cgcgggtttc ctgctcgaga tggccggccg gtcttcgggc
     1141 ggtgcttcga tcgaactgcc gatgtcgcgt caggacatcg ccgactatct cggcctgacc
     1201 atcgagacgg tgtcgcgaac cttcacgcaa ttcgttcagt ccggcctcat ttcgctcgag
     1261 acatcgcgtc gcattcagtt ccgcgatcgc gccgccttga accggctgaa cgcttaaaga
     1321 gcacggcaat ccaatcgcga aaggccggct gcgcgaatcg cagccggcct ttcgcgtcag
     1381 tgcttaccat cgcgctggtg tccgccccgc atcaagcacg gggcgggctt ttttccggat
     1441 catgcgcaag tcgatcacaa gcgaatcaca gaaccgtcgt acaggatgta atccgctatt
     1501 gcgtgacggc gtgccgggtt taaatagagc cgcatcacat aaaggacccg ctatgaccgc
     1561 accccgacgc gacctcgccg catcgatcga ccccgcaaag ctcgaccgcc ttgccgaagt
     1621 cgccgtgaaa gtgggcttgc gcctgcgcga gggacaggat ctgctgctca cggcgcccgc
     1681 ggcggcgttg ccgctggtgc gtaaagtggc cgaacacgcc tacaaggcgg gcgccggggt
     1741 cgtgacgccg atcctgtcgg atgaggaatt cacgctggcg cgctatcgtt tcggccacga
     1801 ggacagcttc gatcgggccg ccgactggct ctatgagggg atggctaaag cgttcgcgca
     1861 aaacaccgcg cggcttgcga tcgtcggcga gaatccgatg ctgctgtcgg gcgaggatcc
     1921 ctcgaaggtg gctcgcgcca gcaaggccaa ttcgaaggcc tatcagccgg cgctggagaa
     1981 gatcgtcaac ttcgacacca actggaacat catcgcttat ccgagcccgt cctgggcgaa
     2041 gctggttttc cccgacgttg ccgaggagat cgcggtcgca aagcttgcgg atgcgatctt
     2101 ttccgcctcg cgcgtcgatg aggatggcgc gattgccgcg tgggaccggc acaatgcgaa
     2161 actgcgcgag cggacggagt ggttgaacgg gcagcgcttc agcgcactgc attattctgc
     2221 ccccggcacc aatctcacca ttggtctcgc cgacggccac gaatgggaag gcggcgcttc
     2281 gaccgccagg aatggtatca cctgcaacgc caatatcccg accgaagagg tcttcaccac
     2341 gccgcattgc cggcgggtca atggccgcgt cgtcagctcg aaaccgctat cctaccaggg
     2401 cacgctgatc gacaacattc aggtcaggtt cgagggggga cgcattgtcg aggccagggc
     2461 ttcgcgcggc gaagaggtct tgaacaaggt gctcgataca gatgacggcg cgcgccggct
     2521 gggcgaagtg gcgctggtgc cgcattcttc gccgatctcc aaaagcgggc tgttgttctt
     2581 caacacgctg ttcgacgaga acgcggcgtc ccacatcgcg ctcggccaat gctattcgaa
     2641 gtgcttcatc gacggcgcca aattgacgcc gcagcagatc gcggcccagg gcggcaacca
     2701 aagcctgatc cacatcgact ggatgatcgg ctccgacaag accgacatcg acggcgttca
     2761 tgccgatggc cgccgcgtgc cggtgttccg caagggtgag tgggcgtaaa caatccgccc
     2821 tctatctccc cgcaaagccc gcttcgcgcc gattggccgt gatgcgttcg ccaatgaccg
     2881 gcggttcgtg cggaatgttg gctgcgccgc tccgcgtcaa tacagcaccg acggcagata
     2941 ggcgccctgc ggctcgtcgt agaggccgat ggtcatcagt gtcatctggt gcaccaggcg
     3001 cagcctgtgc gcgaggcacc agcgaaacaa ttcgctgttg cggctcggca gcagaaagcc
     3061 gccgccgggg aacatttgcg ccgccccgat cagtgctttc agcccgttgt tgttgtccgc
     3121 gaccgcgtgc gcaaagaaag cgatgtcgct ggcgtaggcc gtgatctcgc cgagatgctc
     3181 gaccacccgg ccggtgcctg ccttgatggc gtcttcgagc tcgccgccgc ggtcgtggcc
     3241 gtgcacgcga cggcacagcg cgttgcaggc ggcgacatct gcggtgcgcg cgggccgtac
     3301 ctcgtaaccg ggcagcttca gattcaaggc cgctccatcg atcttcgaca cggtttcgcg
     3361 gctgtcgaag ccgagtttgg tgtagaggca gagcgagcga ttgtggaaag ccgattgcaa
     3421 cagccgcacg ccggcggctt tgcgcgcgcg ggcgcggtcg agcacggcca gcatcaggct
     3481 cttgccgata gtctggttct ggatcgaggg atcgaccgtg atcgggccga caccggcgat
     3541 cgggccccgc tcgtcgagaa aattgctgcc gacgatcttg ccgtccttct ccgcgaccac
     3601 cgaatagaag cctccatgcg ccagcagcat ggtcagcaca cccgttgcga tttccggcga
     3661 agggaaatcc ggcggaaaat tatgggtgtc gttaatggat ttgaaggcgc gaaaacagat
     3721 cgggccgcac acggcggcat ccgccggcgt gccggcacgc aacacgatgc tcatgcttga
     3781 aatcccctgc cgttatgaaa tatcggcgat gctaggcgaa cctccacaaa tttcaagcaa
     3841 gcggtaggcc tcatcgagcg agcccgcgta aaagtgcagg acattttttc cggatcatcc
     3901 gctggccttc ccgcgctttt gcgcgtagtt tcaggacact tggcggtggc acggatgcac
     3961 atattctcaa gcagattaat tctcgcagtc gtagtggcgg cgctttatcc gggcgggcga
     4021 atagcccacg cccaagccgg ccctatgtcc tattggactc caggctggcc ggtcggcttc
     4081 agcggtgatt ttgcgacagg acaaggcgcg aacacctacg ccaacttccc ggcctttaac
     4141 ggcagtgacg ccggcggctt cggctatgcg cgctacaatt ttcagaacgg ctggtttgtc
     4201 ggcagcgaac gcggcggcat gggcttgagc acgagcggct tcaatcagat cggagccttc
     4261 ggcaactcgc tctcttacga aggcttgcag ttcggctaca acttccagaa gtcgactggt
     4321 ttgccgctca cgatctacgc cggcttcgac accttgaaat acacgaccgg catcggtagt
     4381 cctttcgccc cattcgactc catgtccggc acgctgcccg gctacagcgc gcgtgcgggt
     4441 gtcgaattcc agccgacgtc gaatgtgagc ctgtcgctgg gattcggcta cacccagcag
     4501 tccgggcgcg tcgacagcga catcaattcg ctctcactac ctggcgctta tcagtttcct
     4561 tacgccggcc gccgctagtg cgcgcggtcg gacgcttggg atgaattgat tcctgacgga
     4621 caagtcagtt tgcgcttttg cgatgggcgc attgtcgcac cgcccataaa gaaatgctta
     4681 aaacgtggcg gctagcttgc cctcccattt ggccgaagag ggttttgcga tgtcgcgcgc
     4741 gctgatcgaa tttggcaaca ccgaggtcga actgacgccg cggccgatcg agcgatcctg
     4801 gatcatcgag ggcgatccgc aagcgagctc gtgcgtgctg tcgcagagcg cggacgggct
     4861 tgccacgacc atggtctggc actgcacagc gggcaagttc aactggtatt acgatttcga
     4921 cgagaccgtc ctgatcctcg acggcggcat cgtgatcgag agcgagggca tgaagccgac
     4981 ccactacggc ccgggcgacg tgatcttctt caaggatggt gcgcatgcca tttggcatgt
     5041 cgaggaccat gtcaggaagc tcgccttctg ccgcaagacc cagccggtct ggctgggctt
     5101 tgcggtgcgc gtattcctca agctgaagaa gatcatgatg gggcccgccg cacgcgagcc
     5161 cgcgagcctg atggcccgct aatgcatgat cgggaaaagc cgtcccgcac ttggatagcg
     5221 ggctggcgcc tcaggcatcc agcttcaggc gatagaactc catcgccgtc ctcgaaaaca
     5281 gcgcgttttt gtcgctttcc gagcaagccg cagcgatgcg cttgaaggca ttgaagatca
     5341 cctgatagct gcactggcct ttgtcgggcg ggaagttgct ctcgaacatg cagcgctgcg
     5401 ggccaaaggc ctcgatacag gtttcgatat agggcttcca tgcggtggcg gcttcttccg
     5461 atgtcggcgg ttttggccgt ctgtcaaagt cgaatccggg caagcgcatc gcaagcccgc
     5521 cgagcttcac cacgacgttc tcgcatttcg cgacctcgcg gatcgaggct ttccaggtct
     5581 tgaagatttc gtcgcgccgg ttggcatagc tggcgatgcc gatgatgccg ccgcaatggt
     5641 cgaggcagat tcttgtgtcc ggaaatgcgc gggcgagatc ggcgaactcg ccaagctgcg
     5701 gatgaaacag ccaggcatcg aagctgagac caagcggcgc aagacaggca aaacccttgc
     5761 ggaaggtgga atcgagcaac agccccttcg gccgcgtcgc atagatgccg gccgcctcct
     5821 tgtccgcgtc ccaggccgag gaatgacgga tgccgcggaa gcggccgccg ccggcggcga
     5881 tctcggcttc cagcaccgcc ttggcgccgt cgccggcgag cagattgacg tggccgacga
     5941 ttcccgcaca gaccaaggcc ttgccatagg cgccgcttgc agccatcgcc gcggcaccgt
     6001 tggcgaactc gacctcgccg acggggcgaa atgcctcggg cccgaaggca cggtacatcg
     6061 agcgggcctc gacataaacg gttgcgacga cgttgtggcc cgatgcgatg tcgtcggtga
     6121 tctcctcgat caggtagcgg aggccgccgc gatcccagag atgatgatgc ggatcgacga
     6181 tggggcgggc cggatcgata atctcttcgg tgtgctgctt gagccagtct tcgcgcggat
     6241 cggcatagag cccactcgcg gagacgggcg cggtgctgac aaccatggtg tttcttccct
     6301 gatgtctggt gtgggcttgt tcttgggatc agaccccgtc gaggataatc accgtcgggg
     6361 ttgtcggcaa tgtctcgcgc gaaatgcgca aggtgcgttc actgcgtctg tcgatctcga
     6421 attccttccc gatccgctcc atgaactctt cgagcggagt ggcgaagcgg tgcatcggca
     6481 acacgacaga ggcgcgcagc ttcttcgtga tgtcggaaat gccgccgagc gacatggtgt
     6541 aggcaccgtc gatcggcacc atgacaatat ccaggcggcc gatcgcggcg aaatgggtgt
     6601 cgtcgagctg gtgatgcaga tggccgagat gaccgatgca caggcccgcg acctcgaaga
     6661 tgaagatcga gttgccgtcc ttgatcatgt cgacgcccga atagtcgccg aaaaagcggc
     6721 ggatatcggt cgtgacgttg cggataaaga cgtcgcctat ccgttccgag atttttgcgg
     6781 ggtccccgtc gtcgccccag ccgtggagca cgtgcggaat gcggggatcg gggaacagcg
     6841 tgtaatgggt cgagtgtgcg cggttcatgg tgaccacgtc gggcagccgt ccggtctggt
     6901 aggcgccgct atagtcggtc gcgacccgca cgccgcccgg cgtatcgatg aaataggtgg
     6961 aatggccgcc ataggtgatc gcgacctcgt cggtgttggc ggccgcgcgc cgcaggctga
     7021 cgggagtcgc gcgtgccggc gctttcgata tcgccaggca ctcgctgcgt ggcgcgttct
     7081 ccgccgcggc gggtgcgatc aggagtccga caaaggcaaa aacgagcgaa acggagcgca
     7141 gcacgatcgg gcccatttgc gtgagcggcg actgtatcgc gtctttcgga acggaactat
     7201 gatgggcgcg atcaacagaa cgtcagaaaa aacataccgg gagctacagt catgatgtca
     7261 cgcgcagcgt cttcgcagcg gcttgagccg ctccgtcatg tcgaggccgg cgtgctcaaa
     7321 atcgcctatt tcgaggcggg acctgcggac ggcccggtcg tgctgctgct gcacggtttt
     7381 ccctacgaca ttcactccta cgtcgatctg gcgccgatgc tggccgaaag gggttgccgc
     7441 gtgatcgtgc cctatctgcg cggctacggc ccgacacgct ttctttctgc ggccacgcct
     7501 cgttccggcg agcaggccgc gatcggcgcc gatctgatgg cgctgatgga tgcgctttcc
     7561 gttccgcgcg cggtcttcgc aggttacgac tggggcggcc gcgcggcttg catcggcgcg
     7621 gcgctctggc cggagcgctg tttcgggctt gtctcggtca acagctacca gatccaggac
     7681 atcgccaacg cgctgtcccc ggcgcgtccc gaacgagagg tgccgctatg gtaccagtgg
     7741 tacttccaga tcgaacgtgg gcgcgcgggg cttgccgccg atcgccgcgg catcacaaga
     7801 atcctgtggc ggcagtggtc cccgaattgg cagttcgacg aggccatgct ggagcggacc
     7861 tcggtcgccc acgacaactc cgactatgtc gacgtcgtga tccacagcta tcgtcaccgc
     7921 tacggtcttg ccgatggcga tccgcgctat gccgacatcc agcgccggct tgcggcactg
     7981 ccgccgatca ccgtgccggc aatcacactc gacggcgatg ccgacggcgt cgttccggca
     8041 accgacggca gtgcgagtgc ggcgaaattc tcaggtcccc gcgtccatcg cgtcatcccg
     8101 cgcgccggcc acaatttgcc gcaggaagcg ccggaggaat tcgccgtggc agtgatggaa
     8161 ttggtgaaaa ggagttgaga cagctttaat cccggcgtgg gctgagcttc caggtcgcgg
     8221 cgacgatcac gaccgtcatc aagccgctga ccatggcggc cgcgggaatc gccaacgcga
     8281 gcgccgccgt ggccgcccag ccgatcgagg agaaggcttc cgcaaaagtc gcaaggcgcg
     8341 acgaggtctg gcggcggcgg aattggctgc gcttggcctg cacacgaaac caaagctgga
     8401 tcgctgtcgc ggaggtggct gcgaccacga caccagccat cgtcaccacc gcctgcaacg
     8461 gggcgacaaa ggcaagcccg gcaaccagcg gcgcgaagat gacgccgatc acgatcagca
     8521 cgacctcgac cttggcgcgc gtgacgcgcg aagggggcag cggcgcggtc gccacgagat
     8581 cggcggcgtc ttcgccggag atggtgagcc aggccaatcc tccggccagt tggcccgccg
     8641 ccatcacgat caccggtgtg atcagcacga ttgcggtcga ttgatcggag aagctgcgcc
     8701 acaagaacag cgcgggcggc acgagataga ggagctgcat caaggtctgc gacatcagcc
     8761 agggatcgcg ttgcagcagc aagaattcct ttcgccgcag cgcctgttgc cgtgagccgc
     8821 cgcgaaacgc cttggcgacg cggccctgtc gggcgctcgc ggactgcgcc gaggcgtttg
     8881 ccgcagtgcc gacaaagcgc ggcgcgagga cgatcatgac gaggccaagc attacgaggc
     8941 cggccgagag aagaagcagc aatattacga agtcgccgag cacggcacgc gccggccacc
     9001 acagaatgct gtcggcatcg ggagcgagcg ctgccgccgc atccgaggtc aggacagcaa
     9061 agcgcgacaa ggtaccgtag gacaggatcg cggcgatctg cagcgcgatc acgaaacctg
     9121 cgccgatgat cgccgccagg atctgcgcga tcagccgcgt gcgc
//
LOCUS       DATLJT010001417         2759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2189314,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001417 DATLJT010000000
VERSION     DATLJT010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189314"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VM910_32585"
     CDS             complement(<1..594)
                     /locus_tag="VM910_32585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI67281.1"
                     /translation="MSILTSLHHVTRYRYDRPVSLAPQVIRLRPAPHCRTRIESYSLK
                     VTPAQHFVNWQQDPNGNWLARFVFPEKTREFTITVDLIADMAVINPFDFFVEPFAETF
                     PFAYPAEFAEELAPYLDAEPAGPRLSAFLASIPRNRQNTVDFLVGLNQRLQREIRYLV
                     RMEAGVQTPEQTLAENAGSCRDSAWLLVQILRHLGLAA"
     gene            complement(707..1855)
                     /locus_tag="VM910_32590"
     CDS             complement(707..1855)
                     /locus_tag="VM910_32590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding peptidase"
                     /protein_id="HVI67282.1"
                     /translation="MKLFECQNCGQPLYFENTKCESCGLRLGYLPKREVVTALEAVDG
                     VWSALAAQGERYRFCANAEHDVCNWLVPADDSEIFCAACRHNRTIPDLANPENLAHWR
                     KIEYAKHRLFYTLLRLQLPLATRSEDPNGLAFDFLSNPGGGAQGQAPVMTGHSGGLIT
                     LNVAEADAPERERQRKSMGEPYRTLLGHFRHEIAHYYWDRLVADSPAIEEFRRLFGDE
                     REDYGASLQRHYADGPPPDWTEHFVTAYASSHPWEDFAETWAHYFHMVDTLETAFAFG
                     LRLRPRVAKGADLAAVIDFDPYAAEMDRIIDAWLPLTFAVNSINRSMGQLDLYPFVLA
                     PPAIWKLTFIHDQIHAVTADRPRDSEHNAIRAIIAGLKRAVGSPEVAG"
     gene            2111..>2759
                     /locus_tag="VM910_32595"
     CDS             2111..>2759
                     /locus_tag="VM910_32595"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HVI67283.1"
                     /translation="MSRRRSFRQALNRAVAERRLLVVPGAYDALSALLIERTGFDTMF
                     VGGFPVAGARYGVPDIGLKGFGDLAAAVRDILAACDVPVLVDIDDGYGDVKNAVHTVQ
                     TYERMGASAVFLEDQAWPKRCGHMAGKKVVPAEIMEAKIRATMAERLDAETLICVRTD
                     ARAVLGFDEALRRAERYINAGAEAIFVEAPESIEELERVARSFDVPQFANPLVGGR"
BASE COUNT          518 a          821 c          904 g          516 t
ORIGIN      
        1 ggctgcgagc cccagatgtc gcaggatttg caccagcaac catgcgctgt cccgacacga
       61 acccgcattt tccgcgagcg tttgctccgg cgtctgcacg ccggcctcca tgcggacgag
      121 atagcggatt tcgcgttgca gacgttgatt gagtccaacc aggaagtcga ccgtattttg
      181 acgattgcgg gggatcgagg cgaggaacgc cgacagccgc gggccggccg gctcggcatc
      241 gagataaggc gcaagctctt cggcgaactc ggcgggatag gcgaacggga aagtttccgc
      301 gaagggttcg acgaagaaat cgaacggatt tatcaccgcc atgtcggcga tgagatcaac
      361 tgtgatggtg aattcgcgag tcttctctgg aaagacgaag cgtgcgagcc agtttccatt
      421 cggatcctgc tgccagttca cgaaatgctg cgccggcgtg actttgagcg agtagctttc
      481 gatccgcgtg cggcaatggg gggccgggcg cagcctaatg acctgcggcg cgaggctgac
      541 cggccggtcg tagcggtagc gggtgacgtg gtggaggctg gtgaggatcg acatacgctg
      601 tcttgctata cgaagactgc ttccatatta cgcgattttg cgatgcaatt tctggaccga
      661 cttgcaccct ctcggccaag gggggcagcc gcggttgaaa atccgcttag cccgcgactt
      721 cgggcgagcc cactgcgcgt ttgagcccgg caatgatggc gcgtatcgcg ttatgctcgg
      781 aatcgcgcgg ccgatccgcg gtcaccgcgt gaatctgatc gtggatgaag gtcagcttcc
      841 agatcgccgg cggggccagc acgaacggat agagatccag ctgacccatg ctgcggttga
      901 tggaattgac ggcaaatgtc agcggcaacc aggcatcgat gatccggtcc atctcggcgg
      961 catacgggtc gaagtcgatc accgcggcca ggtccgcgcc cttagctacc ctgggccgca
     1021 gccgcagacc gaatgcgaac gcggtttcga gcgtatcgac catatgaaaa tagtgggccc
     1081 aggtttcggc gaaatcctcc cacggatgcg agctggcata ggcggtgacg aagtgctccg
     1141 tccagtcagg cggcggaccg tcggcatagt gtcgctgcag tgacgcccca tagtcctcgc
     1201 gctcgtctcc gaacagtcgc cggaactcct cgatcgcggg ggagtccgca accaggcgat
     1261 cccagtaata atgcgcgatc tcgtgccgga aatgaccgag cagcgtgcga tagggttcgc
     1321 ccatcgattt gcgctggcgt tcgcgttcgg gggcgtcggc ctccgccacg ttgagggtaa
     1381 tgaggccgcc actgtgccca gtcatgaccg gcgcctgacc ctgcgcgccg ccgcccgggt
     1441 tcgacagaaa gtcgaatgcg agaccgttgg gatcttccga cctggttgct agcgggagct
     1501 gcagccgcag cagcgtataa aagagtcgat gcttggcgta ttcgatcttg cgccaatgcg
     1561 cgagattctc gggatttgcg agatcgggga tggtgcgatt atggcggcag gccgcacaaa
     1621 aaatctcgct gtcgtccgca ggcacaagcc agttgcagac atcgtgctcg gcgttggcgc
     1681 agaagcgata gcgctccccc tgcgccgcga gcgcgctcca gacgccatcg actgcttcca
     1741 atgcagtgac gacttcccgc ttcggcagat aacccagccg caggccacag ctttcgcact
     1801 tcgtattctc gaaatagagc ggctgaccgc aattctggca ctcgaagagc ttcatgggtc
     1861 ccggtcactc tgatggtcgc tgcagattgg cagcgcggtc atggcataac gtttgagtat
     1921 agcggcttcg gacgcgacgc gtgaggcggg tggtctctac ttcaccgggc gatcattcat
     1981 ctccgatacg accctgcctg agcggctgat ccgtgagcga ttcggccgtg gtaatcatga
     2041 gctgcgcggg cccgccaagc cggcctatca taccaagtcg gcagccgttg ccggcgatta
     2101 ggagatagcc atgagccgtc gaagaagttt tcgccaggcg ctcaaccgag ccgttgcgga
     2161 gcggcgattg ctcgtcgttc ccggcgccta cgatgcactg agcgcgctgc tgatcgagcg
     2221 gaccggattc gacaccatgt tcgtcggtgg cttcccggtc gccggtgcgc gctatggcgt
     2281 gcccgacatc ggcctcaaag gcttcggcga tctcgcggca gcggtgcgcg acatcctcgc
     2341 ggcctgcgat gtgccggttc tggtcgatat cgacgacggt tacggcgacg tcaagaacgc
     2401 cgtgcacacc gttcaaacct acgaacgcat gggtgcgagc gcggtctttc tcgaggacca
     2461 agcttggccc aagcgctgcg gccatatggc cggcaagaaa gtcgttccgg cggaaatcat
     2521 ggaggccaag atcagggcca cgatggcgga gcggcttgat gccgagacgc tgatctgcgt
     2581 gcgcaccgat gcgcgcgcgg tgctgggctt cgacgaggcg ctgcggcgcg ccgagcgcta
     2641 catcaacgcc ggcgccgagg cgatcttcgt cgaggcgccg gagagcatcg aggaactcga
     2701 acgcgtcgcg cgcagtttcg acgtaccgca attcgccaat ccgctggtcg gcgggcgca
//
LOCUS       DATLJT010001418         5156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1959996,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001418 DATLJT010000000
VERSION     DATLJT010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959996"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VM910_32600"
     CDS             <1..1534
                     /locus_tag="VM910_32600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HVI67284.1"
                     /translation="VDQRRGRAGRTEPGICYRLWDEPQTASLPAFTQPEILSADLSSL
                     VLDLAQWGVNDPATLAFLDPPPAPALKEAKSLLHELGALDFDGRITAEGKRLRALAMP
                     PRLARMIVDAQDFDAAEAAAEIAAVLTERGLGGDSVDLDARLDQFRRDRSQRATSARE
                     LARRWASQVASSPARLASRGERSPARTDGLSTGVMLAFAYPDRVARNRGNGSFVLANG
                     RGAAVEQTSALARAPYIAVGELSGTASSGRILLAAPISQEEIEAHFADHIETSDEMSF
                     DRAALALRARRRKTLHAITLSERPLALKPSAETARILADGLIAAGLDSLPWSKSAKQW
                     RDRVMFLRKAEGESWSDLSDDALRSRREDWLVPALHDKTALKDLSAGELSDALMALLP
                     WDLRARLDREAPTHFEAPTGSMLAIDYEAEQGPTIAVRLQELFGLNAHPSIAKGRVPL
                     VLELLSPAHRPVQVTRDLPGFWRGSYAAVRADLRGRYPRHSWPENPAAALPTRRAKPR
                     GT"
     gene            1663..2181
                     /locus_tag="VM910_32605"
     CDS             1663..2181
                     /locus_tag="VM910_32605"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HVI67285.1"
                     /translation="MRNILITAAILAGLGTFLAQMADKIAPAPASATIAVTQAAPAAA
                     PAESGFRALNVPRDARGHFATEGRIDGQRINFMIDTGASVIALNESSAARFGLRPTPG
                     QYNATVTTANGTVKAARARLAMVEVGGLVVRDVDAMVLPDEALSENLLGLSFLSKLKR
                     FEYANGQMVLEQ"
     gene            2348..3847
                     /locus_tag="VM910_32610"
     CDS             2348..3847
                     /locus_tag="VM910_32610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HVI67286.1"
                     /translation="MFPKPKSVLLPNTSAFESEPMVKATGFREYDARWLFNKEINLMG
                     VQALGMGLGALIAELGARQEIVTGHDFRGYSASIKYALISGLLAAGCKVHDIGLALTP
                     MAYFAQFELDVPCVAMVTASHNDNGWTGVKMGANRPLTFGPDEMTRLKEIVLNADFKT
                     RAGGSYQFHENFPARYVADLTGRTRLKRKLKVVVACGNGTAGAFAPQVMEKIGCDVVP
                     LDTELDYTFPKYNPNPEDLEMLHAIRDAVLASKADVGLGFDGDGDRCGVVDNTGEEIF
                     ADKVGVMLARDMSAIHKDAQFVVDVKSTGLFLTDPVLQKQGAKASYWKTGHSYMKRRT
                     NELGALAGFEKSGHFFFNKPFGRGYDDGLVSAIAICEMLDRAPGKSMADLKNALPKTW
                     SSPTMSPHCADEVKYNVVDAVVKHFEAARQKGEKVAGQAIRDLVTVNGVRVTVEDGSW
                     GLIRASSNKPELVVVVESPVSEQRMRDMFAAMDSVLRTHPEVGEYNQKI"
     gene            complement(3861..4370)
                     /locus_tag="VM910_32615"
     CDS             complement(3861..4370)
                     /locus_tag="VM910_32615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HVI67287.1"
                     /translation="MSELVTQLPEPNNRRLDAIDRKILMVLQEDASLSVAEIGDRVGL
                     SSTPCWKRIQRLEADGVILRRVALVDQNKIGLGISVFVSVESADHSEAWLKKFADAVS
                     AMPEVMEFYRMAGDVDYMLRVVVADMQSYDIFYKRLIATVPLKNVASRFAMEKIKSVT
                     ALPVPAVAQ"
     gene            complement(4442..>5156)
                     /locus_tag="VM910_32620"
     CDS             complement(4442..>5156)
                     /locus_tag="VM910_32620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVI67288.1"
                     /translation="EQLAHEGIDTSHIVHMHGLVTPISNIMIDPSGERTIVTFRDPEL
                     WKVRLPPADELLKDCHAILTESRCAAFCTDLCAEACRRGIPVVVDVDHAMSLREGLLT
                     ASTHLVFSSEPLQETAGVGDDGEALRKLAKLTPSFLAGTRGPRGTIWLDEDGELQETP
                     AFPVHTVDTLGAGDVFHGAFTLAITEGQELREALRIASAAAALKCTRFGGAFAAPQRA
                     EVQEFLRQNAAPPAARSPQ"
BASE COUNT          928 a         1616 c         1655 g          957 t
ORIGIN      
        1 agtcgatcag cgtcgcggcc gcgccggtcg aaccgagccc ggcatctgct atcggctgtg
       61 ggacgagccg cagaccgcat cgctgcctgc cttcacccag ccggagattt tgagcgccga
      121 tctgtcgtcg ctggtgctcg atctcgcgca atggggtgtc aacgatcccg cgactttggc
      181 gtttctcgat cccccgcctg cgcccgcgct gaaggaggcg aaaagcctgt tgcatgaact
      241 tggcgcgctc gacttcgacg gtcgtatcac cgcggaaggc aaacggttgc gcgcgctggc
      301 catgccgccg cggctcgcac gcatgatcgt ggacgcgcag gatttcgacg ctgcggaggc
      361 tgccgccgag atcgccgccg tgctgaccga gcgcgggctc ggcggcgaca gcgtcgatct
      421 cgacgccaga ctcgatcagt tccgccgcga tcgctcgcag cgcgcaacga gtgcgcgcga
      481 gcttgcacgg cgctgggcgt cgcaggtggc ttcctctccc gcccgccttg caagtcgggg
      541 agagaggtct ccggcaagga ccgacggttt gagcacgggc gtgatgctgg cctttgccta
      601 tccggaccgt gttgcacgca accgcggaaa cggcagcttt gtgctcgcca atggccgtgg
      661 cgccgcggtg gagcagactt cggcgctggc gcgcgcgccc tatatcgcag tcggcgaatt
      721 gtcgggcacg gcttcgagcg ggcgcatcct gctggcggca ccgatctcgc aagaagagat
      781 cgaggctcac tttgccgatc atatcgaaac atcggacgag atgagtttcg atcgtgccgc
      841 actggcgctt cgcgcacgtc gcaggaagac gctgcatgcg atcacgctgt cggagcggcc
      901 gcttgcgctc aagccttcgg cggagacggc gcggattctg gccgacgggc tgatcgccgc
      961 cggcctcgac tcattgccgt ggtcgaaatc cgcgaaacaa tggcgcgatc gcgtgatgtt
     1021 cttgcgcaag gccgagggcg aaagctggtc cgatctgtcg gacgatgcgc tccgttcgcg
     1081 gcgcgaggat tggctcgtgc cggcgctcca tgacaagacc gcgctgaagg atctttctgc
     1141 cggcgaactg tcggatgcgc tgatggcact gttaccctgg gacttgcgtg cgcggctcga
     1201 tcgcgaggcg ccgacccatt tcgaagcgcc gacaggttca atgctcgcga tcgactacga
     1261 ggctgagcaa ggaccgacga tcgcggtcag gctgcaggaa ctgttcggcc tcaacgccca
     1321 tccctcgatc gccaaaggca gggtgccgct ggtcctggaa ctgttgtcgc cggcgcaccg
     1381 gcccgtgcag gtgacgcgcg atttgccggg tttctggcgc ggcagttacg ccgcggtgcg
     1441 tgccgatctg cgcggccgct atccgaggca ttcctggccc gagaacccgg cggcggcgct
     1501 accgacccgg cgcgccaagc cgcgggggac gtaaagcgag gggcgttaac acttcgctca
     1561 tccttttcgc aaaaatggca gctttcgcgc cggcggctta gtcgcggtgt ggtggcgcaa
     1621 gtggtgaaat tggccgccta aaatcacgtt gagtgtggcg tcatgcgtaa cattctgatc
     1681 accgcggcca tcctggccgg tcttggcacc ttcctggcgc agatggcgga caagatcgcg
     1741 cctgcgcccg cttccgccac cattgccgtc acgcaagccg cacccgccgc agcgccggcg
     1801 gaatctggct ttcgcgccct caacgttccg cgcgacgcac gcggtcattt cgcgaccgaa
     1861 ggccgtatcg acggccagcg catcaacttc atgatcgata ccggtgcctc cgtgatcgcg
     1921 ctgaacgaga gctccgcggc gcgctttggg ctgcgtccga cgcccggcca atacaatgcc
     1981 acggtcacca ctgccaatgg cacggtgaaa gccgcccgcg cgcggctggc gatggtcgag
     2041 gtcggcggtc tcgtcgtgcg cgatgtcgac gccatggtgc tgccggacga ggcgctgtcg
     2101 gaaaacctgc tcggcctgtc cttcctgtcg aagctgaagc gcttcgaata cgccaacggc
     2161 cagatggtgc tggagcagta gacgggttgg atcacctgct tttgcagccg atcgcgagtt
     2221 ttgcaacttt gtccgtgccg cccgaattat agtcaccaga acggcacaag ccgccttccg
     2281 ctggccgcgt ccattcgcta tggctgcgtg atagcgttct tttttgtgtt cccccacgag
     2341 gccttgcatg tttccgaagc cgaaatccgt gctcctcccc aacacctctg cctttgaatc
     2401 cgagccgatg gtgaaggcga ccgggttccg cgaatacgac gcgcgctggc tgttcaacaa
     2461 ggaaatcaac ctgatgggcg tgcaggcgct cggcatgggg ttgggcgcat tgatcgcgga
     2521 gctgggcgcc agacaggaga tcgtcaccgg tcacgacttt cgcggctatt cggcttcgat
     2581 caaatatgcg ctgatttccg gcctcttggc cgcgggctgc aaggtgcacg acattggact
     2641 ggcgctgacg ccgatggcgt atttcgcaca gttcgagctc gatgtcccct gtgtcgcgat
     2701 ggtgaccgcc tcgcacaacg acaacggctg gaccggcgtg aaaatgggcg ccaaccggcc
     2761 gctgactttc ggtccggacg agatgacccg gctgaaagag atcgtgctca atgccgactt
     2821 caagaccagg gccggcggct cctatcaatt tcatgaaaac tttccggcgc gctacgtcgc
     2881 cgatctcacc ggccgaacca ggctgaagcg caagctgaag gtcgtggtcg cctgcggcaa
     2941 cggcaccgca ggcgccttcg cgccgcaagt gatggagaag atcggctgcg acgtggtgcc
     3001 gctcgatacc gagctcgact acacctttcc gaaatacaat cccaacccgg aagatctgga
     3061 gatgctgcac gcgatccgcg atgcggtgct tgcgagcaag gccgatgttg ggctcggctt
     3121 cgacggcgac ggcgaccgct gcggcgtggt cgacaatacc ggcgaggaga ttttcgccga
     3181 caaggtcggc gtgatgctgg cgcgcgacat gtcggccatc cacaaggacg cgcagttcgt
     3241 ggtcgacgtg aagtcaaccg gcttattcct caccgatccc gtcttgcaga agcagggcgc
     3301 caaagcgagc tactggaaga ccgggcattc ctacatgaag cgccgtacca acgagctcgg
     3361 cgcgctcgcg ggcttcgaga aatccgggca cttcttcttc aacaagccgt tcggccgcgg
     3421 ctatgacgac ggcctggtgt cggcgattgc gatctgcgaa atgctcgatc gcgcgcccgg
     3481 aaaatcgatg gccgatctga agaacgcgct gccgaaaacg tggtcgtcgc cgaccatgtc
     3541 gccgcattgc gccgacgagg tgaaatacaa cgtcgtcgat gcggtggtga agcatttcga
     3601 agcggcgcgg caaaagggcg agaaggtcgc aggacaggcg atccgcgacc tcgtcaccgt
     3661 gaacggcgtg cgcgtgaccg ttgaggacgg aagctggggc ctgatccgcg cctcatccaa
     3721 caagccggag ctcgtggtcg tggtcgagag cccggtgtcg gagcagcgca tgcgcgacat
     3781 gttcgcggcg atggacagcg tgctgcgcac ccatcccgag gtcggcgaat acaatcagaa
     3841 aatttgagga gagacgctat ctattgcgcc accgcgggta cgggaagcgc ggtgaccgat
     3901 ttgatcttct ccatcgcaaa gcgcgaggca acgtttttca gtggcacggt cgcgatcagc
     3961 cttttgtaga agatgtcgta gctctgcata tccgcgacca cgacgcgcag catgtagtcg
     4021 acgtcgcccg ccattcggta aaactccatc acttcgggca tcgcgctgac ggcgtcggcg
     4081 aacttcttca gccaggcttc ggaatgatcg gcgctctcga ccgagacgaa caccgaaatg
     4141 ccgagcccga tcttgttctg gtccacgagt gcgacccggc gcaggatcac gccgtcagcc
     4201 tccaaccgct ggatgcgctt ccagcaaggc gtcgatgaca gtccgacccg gtcgccgatc
     4261 tcggcgactg agagggaggc gtcttcttgc agcaccatca gaatcttgcg gtcgatcgcg
     4321 tcgaggcggc gattgttggg ttcggggagt tgggtgacca attcactcat gagaagaact
     4381 ttgttccatt tgaggggata taaaccctca tatagagaaa attcttctat atcaagcccc
     4441 attattgcgg actacgtgcc gccggcggtg ccgcgttttg ccgcaaaaac tcttgaactt
     4501 cagcacgttg cggtgccgca aaggcgcccc cgaagcgggt gcatttcaag gctgcggccg
     4561 ccgaggcgat gcgcagggcc tcgcgcagct cctgtccttc ggtgatcgcc aaggtgaacg
     4621 cgccgtggaa cacgtcgccg gcccccagcg tatccaccgt atgtaccggg aaggccgggg
     4681 tctcctgcaa ctctccatcc tcgtcgagcc agatcgtgcc gcgcgggccg cgggtgcccg
     4741 ccagaaaaga gggcgtcagt ttcgcgagct tcctcaatgc ctcgccgtcg tccccgaccc
     4801 cggccgtctc ctgcaggggc tcgctggaaa acaccagatg cgtggaggcg gtcagaagtc
     4861 cttcacgcag cgacatcgcg tgatcgacat cgacgaccac cggaataccg cgccggcagg
     4921 cttcggcgca gaggtcggtg caaaacgccg cgcagcggct ttcggtcagg atcgcgtggc
     4981 agtccttcaa gagctcgtcg gcaggcggca gtctaacctt ccacaattcc ggatcgcgga
     5041 aggtgacgat ggtacgttcg ccgctcgggt cgatcatgat gttcgagatc ggcgtcacca
     5101 ggccgtgcat gtgaactatg tggctggtgt cgatcccctc atgtgccaac tgctcg
//
LOCUS       DATLJT010001419         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1500538,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001419 DATLJT010000000
VERSION     DATLJT010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500538"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..526
                     /locus_tag="VM910_32625"
     CDS             113..526
                     /locus_tag="VM910_32625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI67289.1"
                     /translation="MKRTPLRIEPISTYLERYRKGPNLRGPIHPVVVAGELVLVSGLP
                     PFDPETGEIARVPFTRQAELVLEQLKLCLETAGSSLTKVLKCNVYCTPDPTHFGAFNE
                     VYDRYFRSDAPARIFVHVPSWPGPFDVEIDCVAVI"
     gene            810..1919
                     /locus_tag="VM910_32630"
     CDS             810..1919
                     /locus_tag="VM910_32630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI67290.1"
                     /translation="MRLGLFMMPVHPPGCVLADTLAEDEEKALYAERLGFDELWLGEH
                     FSATTEPIPSPLMFMAGLVPRTKNLTFGTAVICLPNHHPVKVAAEVAQFDHMSRGRFM
                     LGIGIGGLYSDFELFDNGDRRVRERKVMESIGMIERIWAQDPPYELKGEFWNVQLKDA
                     IIPELGIGFMPKPYRKGGPPISMSVSSRNSPTARVAAQRGWGIISANNVPRPALPAHW
                     ETYSKACADAGKQARGENWRVARNVMVAASEAEARDRVFGEHGSNRYFYTYMREVLSR
                     VGLLGSLKPHAEMADEEATVDVIIEGSVLYGSAKTFLDKLVALRDEAGPFGTLLMTGL
                     DWSGPNRAWERESMRLLAQEVMSKFRQHVAAKAAE"
     gene            complement(2081..2195)
                     /gene="rrf"
                     /locus_tag="VM910_32635"
     rRNA            complement(2081..2195)
                     /gene="rrf"
                     /locus_tag="VM910_32635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2354..>2506)
                     /locus_tag="VM910_32640"
     rRNA            complement(2354..>2506)
                     /locus_tag="VM910_32640"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          492 a          762 c          768 g          484 t
ORIGIN      
        1 gggccggcaa ccaacgacag ttcggcacgc gccgaaccgt ttacgcgcga cagacggtgc
       61 ggccgccgct atcgctgaag tccgccacgc cccatgcccc ttggaggaca tcatgaaacg
      121 cacaccactt cgcatcgagc cgatctcgac ttacctggaa cgttaccgca aaggtccaaa
      181 tctgcgcggt ccgatccatc cggtcgtggt cgcgggcgag ctcgtccttg tctcgggtct
      241 accgccgttc gacccggaaa ccggcgagat cgcgcgcgtg ccgttcacgc gccaggccga
      301 gctggtgctc gaacagctca agctgtgctt ggagacagcc ggctcgtcgc tgacaaaagt
      361 cctcaagtgc aacgtctact gcacgccgga cccgacgcat ttcggcgcct tcaacgaagt
      421 ctacgaccgg tattttcgta gcgacgcgcc ggcgcgaatc ttcgtccacg tcccgtcatg
      481 gcccggaccg ttcgacgtgg agatcgattg cgtcgcggtg atctgaagtg ggcgtgtcgc
      541 accctcgaaa cgttcaggct tcggctgcta cgttcctaaa cgcgttcatg gatgttttct
      601 ccgcgactgg cgctcgccat tcgcgcgccc gccgccgcgt tgtcaaggta cataagggat
      661 cgtagctttg gttggttagg gcgtaatccg tcatcctgag gtgcgcgccg caaggcgcgc
      721 ctcgaaggat gcacggcctc ggccgtcgcc cttcgaggct cgctgcgctc gcgcctcagg
      781 gtgacgaaac aacagcggag cagaccccca tgcgcctcgg actgttcatg atgccggtgc
      841 atccgcccgg gtgcgtgctc gccgacacgc tcgccgagga cgaggagaag gcgctttatg
      901 ccgaacggct cggcttcgat gaattgtggc ttggcgagca tttctccgcg actaccgagc
      961 ccattccgtc gccgctgatg ttcatggcag gactcgtgcc gcgcaccaag aatctgactt
     1021 tcggcaccgc cgtgatctgc ctgccgaacc atcacccggt caaagtggcc gccgaggtgg
     1081 cacagttcga tcacatgagc cgcggccgct tcatgctcgg catcggcatc ggtggcctct
     1141 attcggattt cgaattgttc gacaacggcg atcgaagggt gcgcgagcgc aaggtgatgg
     1201 aatcgatcgg catgatcgag cgcatctggg cgcaggatcc gccctatgag cttaaaggcg
     1261 agttctggaa tgtgcagctc aaagacgcga tcattcccga gctcggcatc ggttttatgc
     1321 cgaaacctta ccgaaaaggc gggccgccga tctcgatgtc ggtgtcgagc cgcaattcgc
     1381 cgaccgcgcg ggtcgccgcg caacgcggct ggggcatcat ctcggccaac aatgtgccgc
     1441 gcccggcgct gccagcgcat tgggagacct acagcaaggc ttgcgccgac gccggcaagc
     1501 aggctcgtgg cgagaactgg cgcgtggcgc gcaatgtgat ggtggcggca agtgaggctg
     1561 aagcgcgcga tcgcgtcttc ggcgaacatg gttcgaaccg ctatttctac acttacatgc
     1621 gcgaagtgct ctctcgcgtc gggctgctcg ggtcgttgaa gccgcacgcc gagatggcgg
     1681 atgaagaagc caccgtcgac gtcatcatcg aaggctcggt gctctacggc tcggcgaaga
     1741 cgtttctcga caagctcgtc gcgctacgcg acgaggctgg cccgttcggc acgctattga
     1801 tgactggcct cgactggagc ggcccgaacc gcgcctggga gcgcgagtcg atgcggctgt
     1861 tggcgcaaga ggtgatgtcg aaattccgcc agcacgtcgc ggcgaaggcg gcggagtagt
     1921 ggccgtccac gtcttatctt ttcgtcactt aagacgtcat gcccggcacg aggccgggca
     1981 tgacgtttaa aagataattt gaaaaacaaa aagccccgcg gaaaacccgc ggggctttta
     2041 ctacagcgat tatcgtgaaa gaggatggcg tccttggcag gcctggcagc gtcctactct
     2101 tccaagcctt aaggcttagt accatcggcg cagaggactt tgacggccga gttcggaatg
     2161 ggatcgggta gaggctcctc gctatgacca ccaggccggc gaaagacgga aatcggaaga
     2221 cggatgtcgg atgacagaaa acattgcgcc atccaaacgt cttcaatgaa gcaagctgac
     2281 tttgatccga cctctgtcat ccgacatctg tcgcatgtcg tccaacaaag gaaatggaca
     2341 tcgactaatg agagcgttca agccaatcga gcgattagta ccggtaagct gaacgcgttg
     2401 ccgcgcttac acacccggcc tatcaacgtg gtggtctacc acggctctca agggagaact
     2461 cgtttcgagg tgggtttccc gcttagatgc cttcagcggt tatccc
//
LOCUS       DATLJT010001420         1848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3569758,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001420 DATLJT010000000
VERSION     DATLJT010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3569758"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(648..947)
                     /locus_tag="VM910_32645"
     CDS             complement(648..947)
                     /locus_tag="VM910_32645"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67291.1"
                     /translation="MHQKLVTEMAARHRLPSISADPDFPKDGGLMYYGARINTIDQFR
                     QAATYVDRILKGAKPGDLPVQLPDRYTLTINLKTAKALGLQIPDKLLALANEVIE"
     gene            1231..>1848
                     /locus_tag="VM910_32650"
     CDS             1231..>1848
                     /locus_tag="VM910_32650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HVI67292.1"
                     /translation="MAREAGLDPDGRIERPGQRSMPVWCTFRDAARKEHLAREAADAA
                     NTIAAEKPQAPQFQNSPLKVFGEHDHATVAQMRNCMSVGNVVAGVVCADGHLGYAQPV
                     GGVIAYEKQISISGVGFDIGCGNMAIRLDTPFNAVEDRVNKIIKDVSRTISFGVGRTN
                     EEQVDHELFDDAEAWAASDMESYRQKAAAQLGTVGSGNHYVDLLRD"
BASE COUNT          357 a          569 c          572 g          350 t
ORIGIN      
        1 gcggcggcaa gcagcctgcg acgactgagt tcaaggctcg attcgttgtc ccgcgtggcc
       61 atggccgcaa ggtcctgcgc aactattgcc ctgcgcgact ctatcacatg ccgagcagcg
      121 tgatgaactc gcgccgcttc aattgatcga attgcattcg aatctccgcc agccagggcc
      181 tgattgcaga atatcccgtc gggatgacgg ccgcgccgat cacgtcaggg gagcgcttct
      241 cacctcgggg gccttagggc atgtcgaatc cataaaagag cagcgatgag gccaagcccg
      301 cctagaacca tgaaatattc cgatcggtgt gcctcgcgtc cagtcagcca gctaattccc
      361 aaaccactgg ccagcaaaat gaacacaagg gcggcgccga cgaggataaa agtctgaatt
      421 cgttcaaggc gggccgttag ggcagacctt tcctagcccg ccgagtatgc tcccgttcgg
      481 tccattcgac tagatcgctc aaattcaatc tgctttccca gggtgtgtgg gccgccgcct
      541 gcaggtctct cgttagacta tcgccgattg ctgcgcgcgc gtcgccaccg gccacgcggc
      601 cgcgctgccg agcagcgtga tgaagtcgcg gcgtttgatc ttcgcggtca ttcgatgacc
      661 tcgttggcaa gagcgagcaa cttgtctgga atttggaggc cgagcgcctt ggcggttttg
      721 aggttgatgg tgagcgtgta cctgtctggc agctggacgg gcagatcgcc aggcttcgca
      781 cccttgagga tgcgatcgac gtaagtcgcc gcttgccgga attggtcgat cgtattgatt
      841 ctggcgccgt agtacatcag gccgccatct ttggggaaat caggatctgc gctgatcgac
      901 ggcaggcgat ggcgcgcggc catctcggtg accagcttct ggtgcaagcg cgtgaacgcg
      961 tcggtcggca ggatcagccc gccgttcgtc cggcatgcag gcgatcgccg ttccggttcg
     1021 cgctacccct gaaatcgagt ccgccttgga gagtttcgca cgccagccgc aacgccttcg
     1081 atcgtccgac cggccgcatc cgccaagctt tttgacaatg aagctctctt tcagcttcac
     1141 gaagccgacg caggcttgcg tcggcaggcg gcaatagcca agtgcaaggc tatggaccga
     1201 cccgatccat accaagagcg tgcgcgggaa atggcccgcg aagctggcct cgaccccgac
     1261 ggcagaatcg agcggccagg ccagcggtcg atgccggtct ggtgcacgtt ccgtgacgcc
     1321 gcgcgcaaag agcacttggc gcgggaggcc gcggacgccg cgaatacgat cgcagccgaa
     1381 aagccgcagg ccccccaatt ccagaacagc ccgctcaagg tgttcggcga gcacgaccac
     1441 gctacggttg cgcagatgcg caattgcatg tcggtcggca acgtcgtcgc cggcgtcgtg
     1501 tgcgccgatg gccatctcgg ttacgcacaa ccggtcggtg gcgtcattgc ctacgagaag
     1561 cagatcagca tttccggcgt cggcttcgac atcggctgcg ggaacatggc catccgtctc
     1621 gatacgccgt ttaacgcggt tgaggaccgc gttaacaaaa tcatcaagga cgtgtcgcgc
     1681 acgatttcgt ttggcgtcgg tcgcactaac gaagagcagg tcgaccacga gctcttcgac
     1741 gatgccgagg cctgggcggc atcggacatg gaatcatatc gccagaaggc ggcggcgcag
     1801 ctcggcaccg tcgggtccgg caatcactac gtcgatctcc tgcgcgac
//
LOCUS       DATLJT010001421         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2190251,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001421 DATLJT010000000
VERSION     DATLJT010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190251"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VM910_32655"
     CDS             <1..510
                     /locus_tag="VM910_32655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI67293.1"
                     /translation="ARGRSVFLERFIHAEIYRARPDVMAVVHTHSPGVIPFTVSKTPL
                     RPVFHNAAFLAAGAPVWDIAREFGQTDMLVRDNGLGKSLARTLSDKSVVLMRGHGDVT
                     VGPAVKIAVFRAYYTDVNARLQAQAIGLGTEVTYLSAEEGKSADTVNFAVIDRVWSLW
                     RRRAQETMK"
     gene            complement(533..2074)
                     /gene="garD"
                     /locus_tag="VM910_32660"
     CDS             complement(533..2074)
                     /gene="garD"
                     /locus_tag="VM910_32660"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HVI67294.1"
                     /translation="MGKREVIAEKPHYIKLNTRDNVAIVVNDFGLPVGTRFPCGLELR
                     SFVPQGHKAALVDIDEGAPILRYGETIGYAAAPIKAGEWVDEARIRMPDAPSLDSLEI
                     ATAVPAPQPPLEGYTFEGFRNADGSVGTKNILGISTSVQCVRGTLEYAVKRIRAELLP
                     KYPNVDDVVPVTHAYGCGVAINAPDAVVPIRTLQNIALNPNFGGEILVVGLGCEKLAP
                     ERLVPEGISDAIVRMQDEAFEGFGAIVDGIMAKADARLAVLNTRRRETCPASDLVIGL
                     QCGGSDAFSGVTANPAVGFAADLLVRAGATVMFSEVTEVRDAIQLLTRRAINEDVGRA
                     LVREMAWYDAYLARGGADRSANTTPGNKKGGLANIVEKSLGSIVKSGSSPIAGVLAPG
                     EKARQKGMLFAATPASDFICGTLQLASGMTLQVFTTGRGTPYGLAAAPVIKVATRSEL
                     ARRWKDLIDFDAGRIATGEMTIEETGWELFRLILDVASGRTKVWSERWGIHNDLTLFN
                     PAPVT"
     gene            complement(2102..>2891)
                     /locus_tag="VM910_32665"
     CDS             complement(2102..>2891)
                     /locus_tag="VM910_32665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVI67295.1"
                     /translation="AISFTGSVATGRKIAQACVTSEPMKKFQLEMGGKNPLVVLDDAD
                     LKVAVEAATNGAYFSTGQRCTASSRLIVTEGIHDRFVAAMTERLKALSVDDALKPGVQ
                     IGPVVDQSQLDQDLRYLKIGKDEGAKLHWGGELLNRETPGFYLQPALFTEATNKMRIA
                     REEIFGPVACVIRAKNYDEALAIANDTDFGLAAGVCTTSLKYASHFKRNSEAGMVMVN
                     LPTAGVDYHVPFGGRKGSSYGAREQGAYAREFYTTVKTAYTNPG"
BASE COUNT          480 a          902 c          964 g          545 t
ORIGIN      
        1 gcgcgcggtc gcagcgtttt tctggagcgc ttcatccatg ccgagatcta cagggcccgg
       61 cccgatgtga tggcagtggt gcacacgcat tcgcccggcg tcattccgtt caccgtgagc
      121 aagacgccgc ttcgtcccgt gtttcacaac gccgcattcc tcgcggccgg tgcgccggtc
      181 tgggacatcg cgcgtgagtt cggtcaaacc gacatgctgg tgcgcgacaa cggcctcgga
      241 aagtcgctcg cgcggacgct gtcggacaag tcggtcgtgc tgatgcgcgg ccatggcgac
      301 gtcacggtcg gccccgcggt caagatcgcg gtgtttcgtg cctattacac cgacgtcaat
      361 gcacgcctgc aggcgcaggc gatcgggctc ggcacggagg taacgtatct cagcgccgag
      421 gagggaaaga gcgcggacac ggtcaatttc gcggtcatcg accgtgtctg gagcctgtgg
      481 cggcggcggg cgcaggagac aatgaagtag cgcccgcgat tccgcgcgtt gttcaggtca
      541 ccggcgcggg gttgaacagc gtgaggtcat tgtgaatgcc ccagcgctcc gaccaaacct
      601 tggtgcggcc gctcgcaacg tcgaggatca gacggaacaa ctcccacccc gtttcctcga
      661 tggtcatctc gccggttgcg atgcggccgg cgtcgaagtc gatcaaatcc ttccagcgcc
      721 gcgcgagctc gctgcgggtt gcgaccttga tcaccggcgc cgccgcgaga ccataggggg
      781 tgccgcggcc ggtggtgaac acctgcagcg tcatgccgga ggcaagctgc agcgtgccgc
      841 agatgaagtc ggatgcaggc gtcgccgcga acagcatacc tttctgacgc gccttctcgc
      901 ctggcgcgag cacgcctgcg atcgggctcg atcccgattt gacgatcgag ccgagcgatt
      961 tctcgacgat gttggcaagc ccgcctttct tgtttccggg cgtggtgttg gcgctgcgat
     1021 cggcgccgcc gcgggcaaga taggcgtcgt accaggccat ctcgcgcacc agcgcgcggc
     1081 cgacgtcctc gttgatggca cggcgcgtca aaagctggat ggcgtcgcgc acctcggtca
     1141 cttccgagaa catcacggtc gcaccggcgc gcaccagaag atcggcggcg aagccgacgg
     1201 cgggattggc ggtgacgccg gaaaacgcat cgctgccgcc gcattgcagg ccgatcacga
     1261 gatcggacgc cgggcaggtc tcgcggcggc gggtgttgag gacggccagc cgcgcgtcgg
     1321 ccttcgccat gatgccgtcg acgatcgcgc cgaaaccttc gaacgcctcg tcctgcatgc
     1381 gcacgatggc atcgctgatg ccttcgggca cgagccgctc aggcgcaagc ttctcgcaac
     1441 cgaggccgac caccaggatc tcgccgccga aattcgggtt gagcgcgatg ttctgcagcg
     1501 tgcggatcgg cacgacggca tccggcgcgt tgatcgcgac accgcaacca taggcatggg
     1561 tcaccggcac cacatcgtcg acattgggat atttcggcag gagttcggcg cggatgcgct
     1621 tgaccgcgta ttccagcgtg cccctgacgc attggacgga ggtcgagatg cccaggatgt
     1681 ttttcgtgcc gacagagccg tccgcgttgc ggaaaccttc gaaggtgtag ccttcgagcg
     1741 gcggttgcgg cgcgggcacg gcggtcgcga tctcgaggct atccagcgag ggtgcgtccg
     1801 gcatgcggat tcgtgcttcg tcgacccatt caccggcttt gatcggagcg gcggcatagc
     1861 cgatggtctc gccataacgg aggatcggcg cgccttcgtc gatgtccacc agtgcagcct
     1921 tgtggccttg cggcacaaaa ctcctgagtt cgaggccgca ggggaaacgc gtgcccaccg
     1981 ggaggccaaa atcgttgacg acgatcgcga cgttgtcgcg cgtgttgagt ttgatgtagt
     2041 ggggcttttc ggcgatcact tccctcttgc ccatcgacgc aactccttca gaggttctga
     2101 tttagcccgg gttagtatag gccgttttga ccgtggtgta gaactcgcgc gcataggcgc
     2161 cctgttcgcg tgcgccgtag ctcgagcctt tgcgcccgcc aaagggcaca tgatagtcga
     2221 cgccggcagt cggcaggttg accatcacca tgccggcctc gctgttgcgc ttgaaatgcg
     2281 aggcatattt caggctggtg gtgcagacgc cggcggcgag gccgaaatcg gtgtcgttgg
     2341 caatcgccag cgcctcgtca taatttttgg cacggatcac gcaggcgacc ggtccaaaaa
     2401 tctcttcgcg cgcaatgcgc atcttgttgg tggcttcggt gaagagcgcc ggctggaggt
     2461 agaagccggg cgtctcgcga ttcaagagct cgccgcccca gtgcagcttt gcgccctcgt
     2521 ccttgccgat cttcagatag cgcaggtcct ggtcgagctg gctctggtcc accaccgggc
     2581 cgatctgcac gccgggcttg agcgcgtcgt cgaccgagag ggccttcagg cgttcggtca
     2641 tggccgcgac gaaacggtcg tggatgcctt cggtgacgat cagccgcgag gaggctgtgc
     2701 agcgctggcc ggtcgagaaa taagcgccgt tggtcgcagc ttcgacggcg accttgaggt
     2761 cggcgtcgtc gagcacgacc agcggattct tgccgcccat ctcgagctgg aattttttca
     2821 ttggttccga cgtcacgcag gcttgcgcga tcttgcggcc cgtcgcaacc gacccggtga
     2881 acgagatcgc c
//
LOCUS       DATLJT010001422         2863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1272436,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001422 DATLJT010000000
VERSION     DATLJT010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272436"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..492
                     /locus_tag="VM910_32670"
     CDS             334..492
                     /locus_tag="VM910_32670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67296.1"
                     /translation="MRELSAEARLHLYARSLVPSERTTTRMHLVALYSALAVIAAIVF
                     GTLSVHPF"
     gene            616..1311
                     /locus_tag="VM910_32675"
     CDS             616..1311
                     /locus_tag="VM910_32675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HVI67297.1"
                     /translation="MARAARLLDLVALLQSRRHAVTGTQLASVLSISRRTLYRDIQTL
                     KALGATIEGEAGVGYLLKPGFVLPPLMFTAEQLEAVALGASMVAHSADEPLALAARDA
                     LAKIAAVLPEDRRNAIEEIGLLSGPRPPARPDGVPLAKLRAAIRSEYRVVIDYADALD
                     RHTTRQIWPVELTFGVQVRLLAAWCELREDFRSFRVDRIIALTETASRYPKRRRSLLR
                     RWRESRGVSPGIL"
     gene            1405..2091
                     /locus_tag="VM910_32680"
     CDS             1405..2091
                     /locus_tag="VM910_32680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI67298.1"
                     /translation="MGNVVRVLSTFALKGAIERLAGQYQTTTETRIAADFAPTLALLK
                     RLREGEVTDVVILTREGLDELRAAGIVVAASCVDLARSYVGLAVKAGHPHPDIATEVS
                     LRKALLNARSVAYGRIGASGIFFAKLIERMGIAAEINAKATVTSGLTAERLITGDADL
                     AVQQISELKQVGGIEVVGPIPHSLQTPGVFSAGRMAASDRIAPAERLLKFLASQEVVP
                     VLRECGLEQP"
     gene            complement(2182..>2863)
                     /locus_tag="VM910_32685"
     CDS             complement(2182..>2863)
                     /locus_tag="VM910_32685"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HVI67299.1"
                     /translation="SVNVACGYHAGDADIMRKTVEMAKARGVSVGAHPGYRDLHGFGR
                     RPFPGMKAAEIENLVAYQIGALQAIATAAGHKVTHVKAHGALSNVACEDDTTAQAIAN
                     AIKAVDPNLVFVVLANSRLVQAGERANLPMVHEVFADRAYEDDGNLVSRRKPGAVLHD
                     ARTIADRVVKMVKDGAVVSITGKVIKTRVDTICIHGDTPGAVEIARGVRKALKDNGIE
                     VAPFKRAV"
BASE COUNT          498 a          881 c          913 g          571 t
ORIGIN      
        1 agcgcatggc caaggatgtg gagcgcgcgc tggtcgatat cgttgctcag ttcggcgcgc
       61 ggtcgacgga tgaagcaatc agcttcattg ccgacctcaa gaagaagggc cggttccagc
      121 aggacgttta ttagcgcgcg ccacgggaac ttggaatccc ctgaagtttc atcaaattcc
      181 gcctaataag attctccgca aaatcctcgg cggagaattt ttcactgcaa tttgaatccg
      241 tcaaagcgtc cgcatcacta tttccggcgc taccttgcag ccggggctga cgcgtatttc
      301 atgtcgggca cgatggggcg atggagaacc accatgcgcg aattgtcggc ggaagcacgg
      361 ctgcatcttt atgcgcgctc gcttgtgccg tcggagcgga cgaccacgag gatgcatctg
      421 gttgcgctgt acagtgcgtt ggccgtgatc gcagcgatcg tgttcggcac gctgtcggtt
      481 caccccttct gatctctctc aaaaccccga ggccggcggc tgccagggtg tttgcccagc
      541 atgcttgcag cagggtgagt ccgaagctag gctcgagcag caaattggct tgatcccctt
      601 cgccctggaa tctggatggc gcgcgctgcc cgactgctcg atttggtcgc gctcttgcaa
      661 agtcgccgac atgctgtgac cggaacgcaa ttggccagcg ttttgtcgat ctcgcgacgg
      721 accctctatc gcgatatcca gacgctgaag gcgctgggcg ccacgatcga aggcgaagca
      781 ggcgtcggct acttgctcaa gcccgggttt gtccttccgc ctctgatgtt caccgccgaa
      841 caactggaag ctgtcgcact tggggcgagc atggttgctc actcggctga cgagccccta
      901 gccttggccg cccgggacgc tcttgccaag atcgcggcag tcctgcccga agatcggcgc
      961 aacgcgattg aagaaatcgg gctgctctca ggcccgcggc cacccgccag gcccgacggc
     1021 gttcccttgg cgaagctgcg tgctgcgata cgctcggaat atcgcgttgt tattgattac
     1081 gccgatgcgc tcgaccggca tacaacgcga cagatctggc cggtcgagtt gacctttggc
     1141 gttcaagtgc ggcttctcgc ggcatggtgc gaactccgcg aggattttcg cagcttccgc
     1201 gtcgaccgca tcatcgccct cacggaaacg gcttcgcgct atccgaagcg cagacggtcc
     1261 ttgctgcgcc gttggcgcga gtcgcgtggg gtttcgccgg gcatcctcta agactggtga
     1321 caggaattgt caccttaccg atctactacg ctggacaggc cgatgcgccg ggcataccga
     1381 gagctaaccg atggagagtg accaatgggg aatgtggtcc gcgtgttgtc gaccttcgct
     1441 ttgaagggcg cgatcgagcg tctggccggg caatatcaga caacgacgga aacaaggatc
     1501 gcagccgact ttgcgccgac gctggcgctg ctaaagcggc tgcgtgaggg cgaggtcacc
     1561 gacgtcgtga tcctgacgcg cgaagggctg gatgaactgc gggccgccgg catagtggtt
     1621 gcggcaagct gcgtcgacct tgcgcgctct tacgttggcc ttgccgtcaa ggccggtcac
     1681 ccgcatcccg acatcgcgac cgaggtatcg ttgcgcaagg cgctgctcaa tgctcgctcg
     1741 gtcgcctacg ggcgcatcgg ggcaagcggc attttcttcg ccaaactgat cgagcggatg
     1801 gggattgcgg ccgagatcaa tgcgaaggct actgtgacct ccggacttac ggcggagcgg
     1861 ctgatcacgg gcgacgcgga ccttgccgtg cagcagatca gtgagttgaa acaggtcggc
     1921 ggcatcgagg tcgtcggccc gatcccacac tccttgcaga cgccgggagt cttttccgcc
     1981 gggcgcatgg cggcgtcgga tcggatcgcg ccggcagagc ggctgctgaa gttcctggct
     2041 tcgcaggaag tggtgccggt gttgcgtgag tgcggtttgg agcagccttg aatttgtcga
     2101 tcgggccgga cgggcggcgg atccaacccg gtcgcgatcg acggcgcgtt ggccgtgatc
     2161 gcggcgacgg tgttctgatc tctatacggc ccgcttgaac ggtgcgacct cgatcccgtt
     2221 gtccttcagc gccttgcgca cgccgcgcgc gatctcgacc gcgccgggcg tgtcgccatg
     2281 gatgcagatc gtatccacac gcgtcttgat aaccttgccg gtgatcgaga ccaccgcgcc
     2341 atccttgacc atcttcacca cgcgatcggc gatcgtcctg gcatcgtgaa gcaccgcacc
     2401 gggcttgcga cgggagacca gatttccgtc gtcctcatag gcgcggtcgg cgaaaacttc
     2461 atgaaccatc ggcaggttgg cacgctcgcc ggcctgcacc agacgcgaat tcgcgagcac
     2521 cacgaaaacc aggttcggat cgaccgcctt gatggcgttc gcgatcgcct gcgcggtcgt
     2581 gtcatcctcg caggcgacgt tggagagcgc gccatgcgcc ttcacatgcg tgaccttgtg
     2641 accggccgcg gtcgcgatcg cctgcagcgc gccaatctgg tacgcgacga ggttttcgat
     2701 ctccgcggct ttcatccccg ggaacggtct gcggccgaag ccgtgaagat cgcgatatcc
     2761 cgggtgcgcg ccgaccgata cgccgcgcgc cttcgccatt tcgaccgtct tgcgcatgat
     2821 gtcggcatcg ccggcatggt aaccgcaggc gacgttgacg gac
//
LOCUS       DATLJT010001423         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3570488,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001423 DATLJT010000000
VERSION     DATLJT010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570488"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VM910_32690"
     CDS             <1..268
                     /locus_tag="VM910_32690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HVI67300.1"
                     /translation="LDRMRVRAFPFHSSVASFIADHDFVYVVEQNRDSQLRSLIVNEN
                     GIDPVRLIPIVHYDGTPITARFIAKAIGDHQDHLKVTPLRKAVS"
     gene            265..1320
                     /locus_tag="VM910_32695"
     CDS             265..1320
                     /locus_tag="VM910_32695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HVI67301.1"
                     /translation="MTYIAKPKFHHPALKKNALGFTHRDYEGKISTLCAGCGHDSITA
                     SIIEACYELSIEPHRVAKISGIGCSSKTPDYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGFGQFAHSIRRGVNMTYIVENNGVYGLTKGQFSATADRGSRS
                     KKGVLNTDNAIDLVAIALQLGATYVARSFSGDKTQLVPLIAGAIQHKGAAFIDVISPC
                     VAFNNHAGSTKSFDYVREHNDAVNRLDVITGRDPITVDYAPGTVQVVEQHDGTKLALR
                     KIDADYDAHDRVGAMAFLAKHAAKGQIVTGLLYVDPESEDLHSHLNTVDAPLNSLEAE
                     DLCPGSATLDKINASLR"
     gene            complement(1493..>2081)
                     /locus_tag="VM910_32700"
     CDS             complement(1493..>2081)
                     /locus_tag="VM910_32700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HVI67302.1"
                     /translation="QPEFDDPRFSGFNPYALGFAIMQDIERIVTRPEDEDREWFPDIA
                     GSGDVMGVLRNVWANYRDESFIGQFLSPRLMRQFRMFHLHDDPEERAGIKVDAIHNER
                     GYRRVRRQLARQYDVGFTGANIEVVDVDLAGDRRLMLRHHVVNGSQLNETDAKRVLQH
                     LADLWSYDVVLTEVDTTDKVLKEFVLNPRALAAAA"
BASE COUNT          392 a          688 c          621 g          380 t
ORIGIN      
        1 cctcgaccgc atgcgggtcc gcgccttccc gttccattcc tcggtcgcca gcttcatcgc
       61 cgaccatgat tttgtttacg tggtcgagca gaatcgcgac agccagctcc gctcgctgat
      121 cgtcaacgaa aacggcatcg acccggtgcg gctgatcccg atcgtgcatt acgacggcac
      181 gccgatcacc gcgcgtttca tcgccaaagc catcggcgac catcaggatc atctcaaagt
      241 gacccccctc cgcaaggccg tgtcatgacc tacattgcaa aacccaaatt ccatcacccg
      301 gccctgaaga agaacgcgct cggtttcacc caccgcgact acgagggcaa gatctccacg
      361 ctctgtgcgg gctgcggcca cgactcgatc acggcctcga tcatcgaggc ctgctacgag
      421 ctgtcgatcg agccgcaccg ggtcgcgaag atttccggca tcggctgctc ctcgaaaacg
      481 cccgactatt tcctcggcaa ttcgcacggc ttcaactccg tgcacgggcg catgccgtcg
      541 gtgttgaccg gcgccaatct cgccaaccgc gacctgatct atctcggcgt gtccggcgac
      601 ggcgactcgg cctcgatcgg cttcggccag ttcgcgcact cgatccggcg cggcgtcaac
      661 atgacctata tcgtcgagaa caacggcgtt tacggcctga ccaagggcca gttctcggcg
      721 accgccgacc gcggctccag atccaagaag ggtgtgctca acaccgacaa cgccatcgac
      781 ctcgtggcga tcgcattgca gctcggcgcc acctacgtcg cgcgcagctt ctcaggcgac
      841 aagacccagc tcgtgccgct gatcgcgggg gcgatccagc acaagggcgc agctttcatc
      901 gacgtgatca gcccctgcgt cgccttcaac aaccatgccg gctcgaccaa gagcttcgac
      961 tatgtccgcg agcataatga cgcagtgaac cggctcgacg tcatcaccgg gagagacccc
     1021 atcaccgtcg attacgcgcc gggcacggtg caggtggtcg agcagcatga cggcacgaag
     1081 ctcgcgctgc ggaagatcga cgccgattac gacgcacatg accgcgtcgg tgcgatggcg
     1141 tttttggcca agcatgcggc caagggccag atcgtcaccg ggcttcttta tgtcgatccg
     1201 gaatcggaag acctgcacag ccatctcaac actgtggatg cgccgctgaa ctcgctggag
     1261 gccgaggatc tctgcccggg ctccgccacg ctcgacaaga tcaacgcgtc cttgcggtaa
     1321 gggatgttga ggtgaggttg aattgcgcgg cctgcactct ttcgccttga aaggcggggc
     1381 ctatcgcatt tgataccctg tggacgcgga cgccgacctt tccgcgtcgt catggccggg
     1441 cttggcgggt ctgtggagca atccgcgact taagtcgcac cgaacctgac gctcacgccg
     1501 ccgcggcgag cgcccgtgga ttcagcacga attccttcag gaccttgtcg gtcgtatcga
     1561 cctcggtcag cacgacgtcg tagctccaga ggtcggcgag gtgctgcagc acgcgcttgg
     1621 catcggtctc gttgagctgc gagccgttga ccacgtggtg gcgcagcatc agccggcgat
     1681 cgccagcaag atcgacgtcg acgacctcga tattggcgcc cgtgaagccg acatcgtatt
     1741 gacgcgccag ttgccggcgc acgcggcgat agccgcgctc attgtgaatg gcgtcgacct
     1801 tgatgccggc gcgctcctcg ggatcgtcgt gcaggtggaa catccggaac tgccgcatca
     1861 gccgtggact gaggaactgg ccgataaagc tttcgtcgcg gtaattggcc cagacgttgc
     1921 gcaggacgcc catgacgtca ccgctgccgg cgatgtccgg gaaccactcg cgatcctcat
     1981 cctcgggacg ggtcacgatg cgctcgatgt cctgcatgat ggcaaagccg agcgcgtaag
     2041 ggttgaagcc ggagaaacgc gggtcgtcga attccggctg g
//
LOCUS       DATLJT010001424         3483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4257905,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001424 DATLJT010000000
VERSION     DATLJT010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4257905"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VM910_32705"
     CDS             complement(<1..207)
                     /locus_tag="VM910_32705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67303.1"
                     /translation="MSEKTYDVPAEWAKRAWVDEAKYREMYARSVDDPKGFWAEQAKR
                     LDWMKTPTKIDNWSFAPGNISIKWF"
     gene            614..1759
                     /locus_tag="VM910_32710"
     CDS             614..1759
                     /locus_tag="VM910_32710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HVI67304.1"
                     /translation="MTDQQNFAAAQSDSADPAVALAKALGQWPKAEPAKPELNGKTSV
                     EELAGELGLKLGDQNELTIRRIRRGKGYSFVRANGAHIRCARTIRRLHAMAVPPAYRE
                     VRYAPDPNSHLQAVGRDAAGRLQYRYHADWEKVREHRKAHRLAKLVAALPKIRRNVSM
                     HLAGEEPTREFALSAVIELIARTAIRPGNESYARLNGTRGATTLLKSNVTLEDDSVVL
                     TFKAKGGKAVKKECNAAKLVRAIGILRTLPGKRMFQYRDAGGAVRTVSTTTVNGFLRE
                     IAGIKISLKDFRTLMASAVVLESLSRITPAASARGRRRQVLEAVRAAADELSNTPAIC
                     RKSYVHDTIVTAFEEGILERFAATMKGCRSQKKREQLLAEVVMAAAA"
     gene            complement(1808..2137)
                     /locus_tag="VM910_32715"
     CDS             complement(1808..2137)
                     /locus_tag="VM910_32715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67305.1"
                     /translation="MTERSRAQARSRPRQLLYQPETTNKCLKDPENQSRRSASYERRS
                     AAMIETGRGRELQNKIERYRALEREVTDSLAAALLRDIVRELEASVEKQASSAGGSAP
                     GERGLVS"
     gene            complement(2383..2796)
                     /locus_tag="VM910_32720"
     CDS             complement(2383..2796)
                     /locus_tag="VM910_32720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HVI67306.1"
                     /translation="MAYWLVKSEPSSWSWDQQVAKGGKGEAWTGVRNFTARQNLVNMK
                     KGDKAFFYHSNEGKEIVGIAEIIKEAYPDPTDKTGKFVCVDIKADKALPKPVTMVEIK
                     AEKKLAAMALVKYSRLSVQPVTADEWKLVCKMGGL"
     gene            complement(2800..>3483)
                     /locus_tag="VM910_32725"
     CDS             complement(2800..>3483)
                     /locus_tag="VM910_32725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVI67307.1"
                     /translation="TPVIACAKGIEHGTHKFMTEIIAEAAPHAMPAILSGPSFADDVA
                     RGLPTAVTLAAKDEALASELVQALGSSTFRPYHTTDVRGVEIGGAAKNVLAIAAGIVV
                     GKNLGASAQAALTTRGFSELARFGRALGARAETMVGLSGLGDLILTCASPQSRNFALG
                     LALGRGEPKPVGKLAEGEFTAPVLIELAASQNIDMPVSKAVAAILANKLTIDAAIEGL
                     LTRPFKAEE"
BASE COUNT          643 a         1121 c         1075 g          644 t
ORIGIN      
        1 gaaccacttg atcgaaatat tgccgggcgc gaacgaccag ttgtcgatct tggtgggggt
       61 cttcatccag tcgagacgct tggcctgttc ggcccagaag cccttggggt cgtcgaccga
      121 gcgggcatac atctcgcgat acttggcttc atcgacccag gcgcgcttgg cccattccgc
      181 cggcacgtcg taagttttct cggacatttg acctcctcca cacagttcat gccggcgaac
      241 gcgggacgct gacgccgtct tcgttgttcg cgcgattatg cgtcggcgcc accttcgccg
      301 acaagccgac aaaataaaac cttcgacgac atgtctgtgt tgagcgagat caagcgacgc
      361 ccgcagccca tcaatcggcc ttcggcgggg cagggctgtc tcaatttttc accaaaccgt
      421 ttggtcggac atcaacgatg ccgggtgaat gggtctcgtg aaaggtagta tataaccgtg
      481 gtcttaacag ttcggactcg caatagggag cataaccccc taaatgccct ccccgggccc
      541 ctccgcagtg gcggggaacg aaaggccgcg aagcggcatt gaccgagcga acaggagcaa
      601 ggcatctaac gccatgacgg atcagcagaa ttttgctgcg gcgcagtccg attccgccga
      661 tcctgccgtg gcgctggcaa aagcgctggg gcaatggccc aaggcggagc cggcgaaacc
      721 tgagctcaat ggcaagactt cggtcgaaga gctcgcaggc gagctgggtc tgaagctcgg
      781 cgaccagaac gaactgacca tccgccgcat ccgccgcggc aagggctatt ccttcgtgcg
      841 cgccaacggc gcccatatcc gctgcgcgcg taccattcgc cgcctccatg cgatggcggt
      901 gccgccggcc taccgcgagg tacgctacgc gccggatccc aattcgcatc tgcaggcggt
      961 gggacgcgat gccgcgggcc gactgcaata ccgttatcac gcggattggg aaaaggtgcg
     1021 cgagcatcgc aaggcgcatc ggctggccaa gctggtcgcc gccctgccca agatccggcg
     1081 caacgtttcg atgcatctgg ccggcgagga accgacccgg gagttcgcgc tttcagcggt
     1141 gatcgaactg atcgcgcgca ccgcgatccg gcccggcaac gaatcctatg cgcggctgaa
     1201 cggcacccgc ggcgccacca cgctcctgaa atccaacgtc acgctggaag acgacagcgt
     1261 cgtgctgacc ttcaaggcca agggcggcaa ggcggtgaag aaggaatgca acgcggccaa
     1321 gctggtccgc gcgatcggca tcctgcgcac gcttcccggc aagcgcatgt tccagtatcg
     1381 cgacgctggc ggcgccgtgc gcacggtgtc gaccacgacg gtcaatgggt tcttgcgcga
     1441 gatcgccggc atcaagatct cgctgaagga tttccgtacg ctgatggcat ccgccgtggt
     1501 actggaatcg ttgtcgcgga tcacgccggc ggcaagtgcc cgcggccgga ggcgccaggt
     1561 gctcgaagcc gtccgcgcgg cggccgacga gctctccaac acgcctgcga tctgccgcaa
     1621 gagctatgtg cacgacacca tcgttaccgc gttcgaggag ggcattctcg agcgtttcgc
     1681 cgcaaccatg aagggttgcc gttcgcagaa aaagcgcgag cagcttttgg cggaagtggt
     1741 gatggcggcg gcggcgtagc tctcccgccc tccaaaggag ggtggcgctc gctggcaata
     1801 acgcgcgtca agacacgagc ccgcgttccc ctggggctga cccgcccgca ctgctcgctt
     1861 gtttctcgac gctcgcttca agctcccgga cgatgtctcg cagcagggct gcggcgagcg
     1921 agtccgtcac ttcgcgctcc agcgcgcgat agcgctcgat tttgttttga agttctctgc
     1981 ctcgtccagt ctcgatcatg gccgcggacc tccgctcata cgacgccgag cggcggcttt
     2041 ggttctccgg gtcttttaga catttgtttg tggtttccgg ctggtaaaga agctggcgcg
     2101 ggcgcgaccg tgcttgcgcg cgagagcgtt cggtcaacgc ttggtcggcg atgccggagc
     2161 ctgcctcaaa agccgaacga gtgccggatt tgccgcagga ccgcgaaggc ggcatcggtg
     2221 taccgcccat cgtaaaaatg tctgtcgagc agcgaggcga tcaccacagc cacggctgca
     2281 ttgaaacacc ctttgaacat gctccaagag tgccagcgac cggtaaagga tgcgtagcgc
     2341 ggaccgagca cggacgcacg agttccgaaa acggaactgt gcctaaagcc cacccatctt
     2401 gcagacgagc ttccactcgt cggctgttac aggctgcacc gaaagccgcg agtattttac
     2461 cagcgccatg gcggcgagct tcttctccgc cttgatctca accatcgtca ccggcttcgg
     2521 cagcgccttg tcggccttga tgtcgacgca gacgaatttt ccggtcttgt cggtcggatc
     2581 gggataggcc tccttgatga tctcggcgat gccgacgatc tccttgcctt cgttggaatg
     2641 atagaagaac gccttgtcgc ccttcttcat gttcacgagg ttttgtcgcg cggtgaaatt
     2701 gcgcacgccg gtccacgcct cgcccttacc tcccttggcg acctgctggt cccacgacca
     2761 gctcgacggt tcggatttca ccagccagta cgccatcgct cactcctccg ccttgaaggg
     2821 acgcgtcaac aagccctcga ttgcggcatc gatcgtcaat ttgttcgcaa gaatcgccgc
     2881 gactgccttc gataccggca tgtcgatgtt ctgcgaagcc gccagctcga tcaacacggg
     2941 cgcggtgaac tcaccttcgg caagcttgcc cacaggcttc ggctcgccac gtccgagcgc
     3001 cagcccaagc gcgaaattgc gcgactgcgg gctcgcgcag gtcaggatca gatcgccgag
     3061 accggacagg cccaccatgg tttcggcacg tgcgccgagc gcacgaccga accgcgcgag
     3121 ctcggaaaag ccgcgcgtgg tgagggcagc ctgtgcggac gcgccgagat ttttgccgac
     3181 cacgatgccg gccgcgatcg ccagcacatt cttggccgcg ccgccgatct ccactccgcg
     3241 cacgtcggtg gtgtgatagg gccggaaggt cgacgagccc aaggcctgca cgagttcgct
     3301 cgcgagcgcc tcgtcctttg ccgccagggt caccgcggtc ggcaagccgc gcgcgacatc
     3361 gtcggcaaag ctcggacctg agaggattgc aggcatcgcg tgcggtgccg cctccgcgat
     3421 gatctcggtc atgaatttat gcgtgccgtg ctcgatgccc ttggcgcagg cgatgacggg
     3481 cgt
//
LOCUS       DATLJT010001425        10587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2652690,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001425 DATLJT010000000
VERSION     DATLJT010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652690"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..976
                     /locus_tag="VM910_32730"
     CDS             170..976
                     /locus_tag="VM910_32730"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI67308.1"
                     /translation="MLLSLIVALWSAAVAQPGVTPEHRPTPPDSDEQRIADLVVASRI
                     LVQEGVLDSFGHVTVRSLNNPNHYFMPRAMPPALVTAKDIVELNLDSVPIDANAPRTN
                     GERFIHGEIYRVRPDVGAIVHSHSPAVIPFSISPDRPLRPVLHMAGFLPARVPVFDHR
                     DLSKDDSSLRGKLMVNNAKLGASLAKTLGNDSVVLIRGHGNAVAGASLPWAVLRAVYT
                     QLNARVQMDAIALGGQVIHLDEDELKMHPVEVFDVDRPWQNLKSRLPKNP"
     gene            complement(1425..2717)
                     /locus_tag="VM910_32735"
     CDS             complement(1425..2717)
                     /locus_tag="VM910_32735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HVI67309.1"
                     /translation="MNSTTAAGEARSTKPFYRDMSVQVFAGMASGALIGWLWPQSAEW
                     MRTLGDLFIRLIAMFVGLIIFCAVVHGIATVREAKKVGRIAIKALIYFEVVTTFALVI
                     GLAMVNLLGPGRGMHVDLQSISGSAIDLYLKTAQQISGSGFLLSVVPHTAISAFTEGN
                     VLQVVFLSVLFAFGLHAIGADGSPMIEMIELTKRIVFKIISYIMWLAPIGAMGAIGFT
                     VGRFGIGSLVSLGAMVAEFYLTCALFICIVLWPIAYWNGIRLWRLARYIRTELLIVLG
                     TSSSESVFPQLVDKLTNLGCEESVVGLVLPTGYAFNHDGTCLYFAAASLFLAQAVGLE
                     LSLGQQLGLLGILLVTSKGGAGVAGSAIVVLVSTLAATGTIPVAAVGIILGVHRLMSS
                     GFVAVNILGNALATIVIASWEGAVDRKLLIERLELGPA"
     gene            complement(2862..3935)
                     /locus_tag="VM910_32740"
                     /pseudo
     CDS             complement(2862..3935)
                     /locus_tag="VM910_32740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            complement(3869..4726)
                     /locus_tag="VM910_32745"
     CDS             complement(3869..4726)
                     /locus_tag="VM910_32745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67310.1"
                     /translation="MMIIDAGVHVWRPEAPDRPWMPVRKAHLPEPLTYEKFSAMMKEA
                     GVEHAILVPPSWEGDRVDYSLEAAQKYPDRFAVMGRFPINKPEERSKLETWRDQRGML
                     GVRLTLHHEWDRTWMQDGTADWFWPAAERLGIPVMLNAPYTHKEIGEVAARHRRLRII
                     MDHLGARTTQKDDLLKAASESTATLAKHSNVYVKLTLVPVFSTAPYPYNNIHPYIRHL
                     VEAFGPKRCFWGTDASAMLERTTCSYGNCVKLFTEHMDFLSASDKEWIMGRGLAECLQ
                     WPQSENRQT"
     gene            complement(4741..5640)
                     /locus_tag="VM910_32750"
     CDS             complement(4741..5640)
                     /locus_tag="VM910_32750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67311.1"
                     /translation="MATATKLKLHTMLGNYPITKPLKSGAVRSDLVDFDFVEVKVANN
                     LFKQVVRDARYDVAELAIVTYLQAKAYGKPYVLLPAVVVSRGQHHTIAYNPQRGALKP
                     SELEGKRVGVRAYTVTTGTWVRGILASDYGLDINKVEWITFEDPHVAEYRDPAFVKRA
                     PQGKELTQMLLDGEIAAGIVGDKLPDPKLKHLIPDAEAAAQSWAQRHHGVPINHMVVV
                     RQGLSRSRPDVVKDIFRQLHASKRAAGLPAGGELDPYRFGVEACRPILEIIIDFCHQQ
                     TLIPRRMSVDELFDDTTRVLAAA"
     gene            complement(5656..6504)
                     /locus_tag="VM910_32755"
     CDS             complement(5656..6504)
                     /locus_tag="VM910_32755"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67312.1"
                     /translation="MPVIDIHPHIVSPDTQRYPLAPLGGTQSTWSSERPTTYQTLIKS
                     MDEAGVDKAAIVHSSTAYGYDNSYVADAVTAVPSRFTAVCSIDVLAPNAVKTFDYWLG
                     RGCTGMRLFTTGSTLPDQATWFVDPKTYPFWEHAAAKNIPVCMQMKQEGLPLLRQIMD
                     RFPKVTIILDHLARAPFADGPPYQAAAEFLELAKYKQVYLKITPVNVTPKSWGRGAPD
                     TFFGKVVDMFGASRIAWSSNFPNSVGTLSEILGAACKAFAFAKPSDQDWIFGKTALTL
                     YPALAK"
     gene            complement(6512..7522)
                     /locus_tag="VM910_32760"
     CDS             complement(6512..7522)
                     /locus_tag="VM910_32760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HVI67313.1"
                     /translation="MTQPPRLKIAIATYGHTKAIKDGTVAIAGVERDFVEVVPIIGAF
                     RRMVRDVEFDVCEMAPTTYMIARALGAPFIALPIFLMRRFHHGGFVVRPDSGIKVPKD
                     LESKKVGVRAYSVTTGVWTRGIFINEYGLDSSKVTWVVDDEEHVTALKLPPNVVHAPE
                     GKSLQGMMKTGEIQAGFTGPAGVGRAGPPISGWDKASEAAAAADTYPELITNVEQVEA
                     DWFRRSGIYPIHGLIVVKDEHIKRYPWLARSLMDAFVAAKKPYLDELKRGHGDSPEDK
                     RYCKFLSLMSDPLPYGMTANRPSIEALITYSLQQKLIPSRPQLGRVFVDIDPVPINRN
                     AE"
     gene            complement(7519..8535)
                     /locus_tag="VM910_32765"
     CDS             complement(7519..8535)
                     /locus_tag="VM910_32765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67314.1"
                     /translation="MCAHRLGFKNGSLRLLRLPIVLFLAAIVSTIAHDHARSQSSFPT
                     KPIHIFIPYGAGGVADLTMRLLAQKLTERTKQQVVIENRPGAAGLLAAKEVLKAPPDG
                     YTLDVIGNGQAIAMSLFKTRPYNVLTDFTFVSITARFEMLLAVKADSPLKNLQDVVAA
                     ARKNPGKLNFGAINPGSTQNLSAHLLKQVSGVDVAIVPFKTTPDLVTAVLRGDIDIGF
                     DFYAGLQSLISDNQVRIIATSGEERNPLLRDVPTAKESGFPDYIVTSWNGLASAAGMP
                     AQTLGVLSQQINAALADPTLQATASALGMDARGSTPEEMRDRMAADVKKWAAVIEKAG
                     IEKQ"
     gene            8869..10137
                     /locus_tag="VM910_32770"
                     /pseudo
     CDS             8869..10137
                     /locus_tag="VM910_32770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(10138..>10587)
                     /locus_tag="VM910_32775"
     CDS             complement(10138..>10587)
                     /locus_tag="VM910_32775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67315.1"
                     /translation="LELGFELRIPNAAAQTRIVSVARIRLDRRKSTQLFKGIICDDIS
                     EFESSHASQPVRSPPPNMGRPQKTAWYRGISQISLGLRVRSWVTAAPCSTGRQRMPHS
                     EKLAVRPRRIYEEPGRSDDELLAFDNVILTPHLAGHHVLMDSETSKI"
BASE COUNT         2129 a         3175 c         3112 g         2171 t
ORIGIN      
        1 cgcgggcctc gagcctgccc ccaacagtcc gagtgagatg cgcagcatca tcgcagcgga
       61 ctacaagaaa tggggtcagt tgatagaggc gacgggcctc aaggccgagt aatgcgaatc
      121 tatcgaagcc aggagtcgac acaccatgtg gcgcgcgcga aaagctttca tgttgctatc
      181 tctgatcgtc gccctgtgga gcgcagccgt ggcgcagccg ggcgtcacgc cagagcatcg
      241 accgacccca cccgattccg atgagcagcg cattgctgat cttgttgtcg cgagccgaat
      301 tctcgtccaa gaaggcgtgc tggacagctt tggacatgtg acggttcgct cgctgaacaa
      361 cccgaaccac tatttcatgc cgcgggcgat gccgcctgcg cttgtgaccg caaaggacat
      421 tgtcgaactt aatctcgata gcgtgccgat cgacgccaat gcgccgcgga cgaacggcga
      481 gcgattcatt cacggggaga tctatcgcgt tcgccccgac gttggggcca tcgtgcatag
      541 ccactcgcca gccgtgattc ccttcagcat ttcgcccgac cgtccccttc gaccggtgct
      601 gcacatggcc ggatttcttc ccgctcgcgt tcccgtgttc gatcaccgcg atctttcgaa
      661 ggatgattcg agcctgcgcg gcaagctgat ggtcaacaat gccaagcttg gcgctagctt
      721 ggcgaagaca ctgggaaacg attccgtcgt tcttatccgc ggacacggca atgccgtggc
      781 tggcgcctct ttgccatggg cggtgttgcg ggcggtgtat acacagctca atgcccgggt
      841 gcagatggac gcgatcgcgc ttggcggtca ggtcatccat ctcgacgaag acgaactcaa
      901 aatgcatccg gtcgaagtct tcgacgtgga ccgaccctgg cagaacctga aaagccggct
      961 tccgaaaaat ccctgattgc ctgtcttgcg gacctcgtca ggcgatgcga ccgtgccacc
     1021 cagcttcaca agctcttcgc gaagcgcagc gatctgcgaa cgaccctccg gatcggcctc
     1081 agggtagtcg tctgtcaacc tgcgccgcct gaaggtcagt caacctgtta acctgcccct
     1141 gttttacgtg ttaaccgcgg taaaccttat ttttcgagat gcgcggctgg cgccccctcg
     1201 cacggatgcc acccgcgccg cttttgccac caggaaggac ccgctcatga ttgccgctat
     1261 tcgtatcgcc tcatcgcaag gacgtgacac ggctggggat acttcgcatt ggttgcaagc
     1321 tcatcgagca gcacccttgc cagatgagca gcgtcatgtc ctatcacagc acggcggatg
     1381 ggtggatgaa tgtaggactc gggtacaatc ggatggaggc gcacctatgc gggtccgagc
     1441 tcgagtcgct cgatcaggag ctttcgatcc acggctcctt cccacgatgc gatcacgatt
     1501 gtcgcaagcg cattgcccag aatgttgacg gcaacaaatc ccgaggacat caagcgatgc
     1561 accccgagaa taattcccac ggcagctacg ggtatcgtgc cggtcgctgc cagagtcgac
     1621 accagcacca cgatcgccga ccccgcgacc ccggcacctc ctttagatgt gacgagcaga
     1681 attcccagca gaccgagttg ctgtcccagc gaaagttcaa gaccgaccgc ctgagcaaga
     1741 aagagcgagg cggctgcaaa atagagacag gtgccgtcat gattgaaggc atagccggtc
     1801 ggcagcacca accctaccac cgattcctcg cagccgagat tggtgagctt gtcaacgagc
     1861 tgggggaaca ccgattcgga ggaactcgtt ccgagcacga tcagcaactc ggtgcgaatg
     1921 tagcgcgcca aacgccacaa cctgatcccg ttccagtagg cgatcggcca aagaacgatg
     1981 cagatgaaca aagcgcacgt caggtagaat tcggccacca ttgcgccgag tgacacaagc
     2041 gagccgatgc caaatcgacc gacggtgaag ccgatcgcgc ccatggcacc gatcggcgcg
     2101 agccacatga tgtagctgat aatcttgaac acgatgcgct tcgtgagctc gatcatctcg
     2161 atcatcgggc ttccatccgc gccgatggcg tgtaggccga aagcaaacag caccgaaagg
     2221 aacacgacct gcagcacatt gccttcagtg aaggccgaga ttgcggtatg gggaacgacg
     2281 ctcaggagaa agccgctgcc gctaatctgc tgcgcggtct tcaggtaaag atcgatcgcg
     2341 gatccggaaa ttgactgtag atccacgtgc atgcctcgac ctggtccgag gagattgacc
     2401 atggctagac cgatgacgag cgcaaaggtc gtcacaacct cgaaataaat cagggccttg
     2461 atcgctatcc ggccgacctt cttcgcctcc cgcactgtgg caatgccatg caccaccgcg
     2521 cagaagatga tcagcccgac gaacatcgca atcagccgga tgaacagatc gccgagggtg
     2581 cgcatccatt ccgctgattg cggccacagc cagccgatca gcgctcccga agccatgccg
     2641 gcgaaaacct gcaccgacat gtcgcgatag aatggcttcg tgctgcgcgc ctctccggct
     2701 gccgtcgtcg agttcatcgc gagtctcttt caattcagag gttctgtagc gggatcaaat
     2761 caaaatcgtt tcaacgtcta gaccgcaatt ccgcgaagaa actgctgccc gaggcgcgcc
     2821 ctcttgacca catcgcttag gctttcttgc gccgtggtcg cctattgttt ttcgaggccc
     2881 ccttccgcaa tgacgcgggc ccatcgctca atttcggagc gcatacggtg gtcgagcggt
     2941 tctgtcgcca tcgggcgcgc atcgaatccg atctccttga acctcgcctt tacatccggc
     3001 tcttcaagaa tttccgttgc tgcccggccg agtacgtcta cagccgctac cggcatgtcc
     3061 ttcggaccgt aaagcgcgtt ccaagaaatc actacgaaat cgggaacgcc ggcttcctgc
     3121 atcgttggca cgtcgggtaa gtcgggatct cggctggccg aagttatggc aagtgcgcga
     3181 atttgtttgg cgccgatttg agatcgcaat ccgccatacg cgttgatgac cacgtctacc
     3241 tcattacgca gcaaagcgac acttagttcg gaggggccgc gataaggcac aacggtgaaa
     3301 tccaggtgtt agatcgatcg cattagcagc gcggcgagat aattagtcgt acctgggccc
     3361 gatgtgccga tggtcagggt gccgggcttt tcctttaccg ctgtcatcca ctgtcgcaga
     3421 gtgctgaacg gcgagttctc gcttgtgacg aagagatagg caaactcgct tactccgacg
     3481 atcggtctca gctccttgcg tgggtccacc ggctccttga ataggctcac gccgatagcg
     3541 gtgttgttgc cagcccataa gattgtgtag ccgtcagctg gcgcattggt gaccgcacgc
     3601 tccgcaatga cacccgctgc gcccggttgg ttttgcacaa gcacggggac acggaagcga
     3661 gcagccaatt tttcggcgag aatacgaagc gcgatatccg atgccgctcc tgcagccaaa
     3721 ggcgttacaa tgcggattgg gcgcgatgga taggacgctt gagcggttgc cggggtggaa
     3781 accaccgtgc cgcaccacag catcatcgct agcaacgcag cccagcgaag acgctttaac
     3841 attatcaatt gctgaagcat gcaaacgatc acgtctgccg attttcggat tgaggccatt
     3901 ggaggcattc ggcgagaccg cgccccatga tccattcctt gtccgaagcg gacaaaaaat
     3961 ccatgtgctc ggtgaacagc ttcacgcagt tgccatagct gcaagtggtg cgctccagca
     4021 tcgctgacgc gtcggttccc caaaaacaac gcttaggtcc gaaggcttcc accagatggc
     4081 ggatgtaggg atgaatattg ttgtaggggt agggcgcggt ggaaaacacc ggcaccaagg
     4141 tcagcttaac gtagacattg gaatgtttgg ccaaagtcgc cgtgctctcc gaggcggcct
     4201 tgagcaaatc atccttctgc gtggtgcgcg cgccaagatg gtccatgatg atgcgcagac
     4261 gccggtgccg tgccgcgacc tcgccgattt ccttgtgcgt ataaggcgca ttcagcatca
     4321 ccggaatgcc gagccgctcc gccgccggcc agaaccagtc ggcggtgccg tcctgcatcc
     4381 aagtgcgatc ccattcgtga tgcagcgtga gccgcacgcc aagcatgccc ctctggtcgc
     4441 gccaggtttc aagcttgctg cgctcctccg gcttattgat cggaaaacga cccattaccg
     4501 cgaaccgatc gggatatttt tgcgccgctt cgaggctgta atcgacgcgg tcaccttccc
     4561 atgaaggagg aacgaggatg gcgtgctcga cgccggcctc cttcatcatc gcgctgaatt
     4621 tctcatatgt cagcggctcc ggaagatgcg ccttgcgaac gggcatccag gggcggtccg
     4681 gcgcttcagg ccgccagacg tgaacgccgg catcgatgat catcatcacc ttttccccat
     4741 ctaagctgca gccagcacgc gcgtcgtgtc gtcgaacagt tcgtcaaccg acatgcggcg
     4801 cgggataagc gtttgctggt ggcagaagtc gatgatgatt tccaaaatcg gccggcaggc
     4861 ctcgacaccg aaacgatagg gatcgagctc cccgccggcg ggcaagccgg cggcccgctt
     4921 gcttgcgtgc aactgccgga aaatgtcctt caccacgtcg ggccgggagc gcgacaaccc
     4981 ttgcctgacc actaccatgt gattgatcgg gacgccgtgg tggcgctgcg cccagctctg
     5041 agcggccgcc tcggcatcgg gaataagatg cttgagtttc gggtcgggca gcttgtcgcc
     5101 gacgatcccg gcggcgattt cgccatcgag cagcatctgg gtgagttcct tgccctgcgg
     5161 cgcgcgcttc acgaaggcgg gatcgcgata ctccgccaca tgcggatctt cgaaggtgat
     5221 ccattccacc ttgttgatgt cgaggccgta atcgcttgcc agaattcccc taacccacgt
     5281 ccctgtcgtc accgtgtagg cgcgcacgcc gacacgcttg ccctcgagct cggagggctt
     5341 gagcgcaccg cgctgcgggt tgtaggcgat ggtgtggtgc tggccgcggc tcacgaccac
     5401 cgcgggcaac aacacatagg gtttgccgta ggccttggcc tgcaagtagg tcacgatggc
     5461 aagctcggcg acgtcatatc tggcatcgcg cacgacttgc ttgaacagat tgttggcgac
     5521 cttcacctcg acaaaatcga aatcaaccag gtccgaacga accgcaccgc tcttgagcgg
     5581 cttggtaatc ggatagttgc ccagcatcgt gtgcagcttg agctttgttg cagtcgccat
     5641 gagagcgttt ccttatcact ttgccaaagc gggatagagc gtgagcgcgg tcttgccaaa
     5701 gatccagtcc tgatcggaag gcttggcaaa ggcaaacgcc ttgcacgcgg cgccgaggat
     5761 ctcggacagc gtgccgaccg agttcggaaa gttcgagctc caggcgatgc gggaagcgcc
     5821 gaacatatcg acgaccttgc cgaagaacgt atccggcgca cccctccccc agctcttggg
     5881 cgttacattg acgggcgtga tcttcagata aacttgcttg tatttggcaa gctcgaggaa
     5941 ttccgctgcc gcctggtagg gtgggccatc cgcgaacggt gcgcgcgcca ggtgatcgag
     6001 gatgatggtg accttgggga agcgatccat gatctggcgc agcagcggga ggccttcctg
     6061 cttcatctgc atgcagaccg gaatgttctt cgccgccgca tgctcccaga atgggtaggt
     6121 cttcggatcg acaaaccagg tcgcctgatc cggcaaggtg ctgccggtgg tgaagagccg
     6181 catgccggtg cagccgcgcc cgagccagta atcgaacgtc ttcaccgcgt tgggcgccaa
     6241 gacgtcgatc gagcacaccg ccgtgaagcg tgacggcacc gccgtcactg cgtccgccac
     6301 atagctgttg tcgtagccat aggcagtgga ggaatgcacg atcgccgcct tgtcgacgcc
     6361 agcctcgtcc attgatttga tcaacgtttg ataggtggtc ggccgctcgc tcgaccaggt
     6421 ggattgcgtg ccgccaagcg gggccagcgg ataacgctga gtatcgggcg agacgatgtg
     6481 cggatgtata tctatgacag gcatggacgt ttcactccgc gttgcggttt ataggaactg
     6541 gatcgatatc gacaaacacc cgaccgagtt gcggacgcga cggtatcagc ttctgctgca
     6601 ggctgtaggt gatcagggcc tcgatgctgg gacgattagc cgtcatgccg tagggcagcg
     6661 gatcgctcat caaggacagg aatttgcaat agcgtttgtc ttccggactg tcgccgtgac
     6721 cgcgcttgag ctcatcgaga tacggctttt ttgctgccac gaaggcgtcc atgagcgaac
     6781 gcgccagcca gggatagcgt ttgatgtgct cgtccttcac gacgatgaga ccgtggatcg
     6841 ggtagatgcc actgcggcga aaccaatcgg cttcgacctg ttcgacattg gtaatcaatt
     6901 ccggataggt atccgctgcg gcagcggcct cgctggcctt atcccaacca ctgatcggcg
     6961 gaccggcacg gccgacaccg gcaggcccgg taaatccggc ctggatctcg cccgtcttca
     7021 tcatgccctg cagtgatttg ccttctggcg cgtgcaccac attcggcggc agcttgagcg
     7081 ccgtgacgtg ctcctcatca tcgaccaccc aggtcacctt tgaggagtcg agcccgtact
     7141 cgttgatgaa gatgccgcgc gtccacactc cggtggtcac cgaataagcg cgtactccga
     7201 cctttttgct ttccaggtcc ttgggcactt tgatgcccga atccgggcgg accacgaagc
     7261 cgccgtgatg aaagcggcgc atcaggaaaa tcggcagcgc gatgaacgga gcgccgagcg
     7321 cacgcgcgat catgtaggtg gtgggcgcca tctcgcagac atcgaactcg acatcgcgca
     7381 ccatgcgccg gaatgcccct atgatgggta cgacctcgac gaagtcccgc tccactcctg
     7441 cgatggcaac cgtcccgtcc ttgatagcct tggtgtgccc gtaggtggcg attgcaattt
     7501 tcagtcgcgg cggctgtgtc attgtttttc gatgcctgcc ttctcgatca ccgccgccca
     7561 tttcttgacg tccgctgcca tgcggtcgcg catttcctcg ggcgtgctgc cgcgggcgtc
     7621 catgccgagc gcggatgccg tggcctggag ggttggatcg gcgagcgcgg cattgatttg
     7681 ctgactgaga acgccgagcg tttgggccgg catgccggcc gccgaagcga ggccgttcca
     7741 gctggtcacg atgtagtcgg gaaaaccact ttccttcgcg gtcggcacgt cgcgcaggag
     7801 cggattacgt tcctcgccgg aggtcgcaat aattctgacc tggttatccg agatcagcga
     7861 ttgcagtccg gcatagaaat cgaatccgat atcgatgtcg ccgcgcaaca ccgccgtcac
     7921 caggtcgggc gttgtcttga agggaacgat cgcgacgtcc acgccgctca cctgcttcag
     7981 aaggtgcgcc gacaggtttt gcgtgctgcc gggattgatg gcgccgaaat tgagctttcc
     8041 cggatttttg cgggcggcgg cgaccacgtc ctgtaggttc ttcagtggcg aatccgcctt
     8101 gaccgcaagc aacatctcga agcgagccgt aatcgacacg aacgtgaagt cggtcagcac
     8161 attgtagggc cgcgtcttga acagcgacat agcgatcgcc tgcccgtttc cgatcacatc
     8221 aagcgtgtaa ccgtccggcg gcgctttgag cacttccttt gccgctagca ggccggcggc
     8281 tcccggacga ttttcgatga cgacctgctg ctttgttctc tcggtcagtt tctgcgcgag
     8341 cagccgcatg gtcaggtcgg ccacccctcc ggcaccatac gggatgaaga tgtgaatcgg
     8401 cttggtgggg aaacttgact gtgatcgagc gtgatcgtgc gcgatcgtgc tgacgattgc
     8461 cgccaagaaa aggacaatgg gcagccgcaa gagtcgcagg ctgccattct tgaagcccag
     8521 gcgatgcgcg cacatctcaa agcctccctg atctttcttt gcttgccgcc gggttacgcc
     8581 accggctttg taagctcgcc ggagagatga cgaccacggt gccaatccag gccaaagcga
     8641 tcggcgggca tccgacggga gggcgagccg ggacccgtaa ccacaggcca tccctcaagg
     8701 ataggccgtt ggttgtctcg ctgcgctggg ctgccggggg gatgacaccg tcgccctgtt
     8761 ccttatctca tagtcgcaca gtgcgggctt tcccgcgcaa ttgaaaccgc gcacggagcc
     8821 ataagttttt gctattcttt gacgcaaagt ccgctgagcg cgaaccccat gcgcacacga
     8881 cccgattctc cttcactgcg ccagcttcgg gcattcgagg cggttgctcg ctgcactagc
     8941 attggtggcg ccgcgaagga gcttggcttg tcgcagcccg cagtgacgca aatgattgcc
     9001 caactcgaga ccgtgctcgc cgtgagcttg ctggagcgtc gccgaagcgg atcgtttctg
     9061 acaccgctcg gatcggtgct tttaccgcgg gttcagcggc tgttcgcaca tattcaatca
     9121 gcgttgtgcg acccaactat cggcgcatcg ctttcaggtc gtgaaacgat caaatcggtg
     9181 gagaataaga ttacggacac gcatgtccgc gctctcaccg cgatcgcaga aagcggctcg
     9241 ttcgaggcgg cggcgcgcag gttcaacata tcccagccgg cgctgcatcg atcggctcgc
     9301 gatctcgagc gcgtggttcg cagaagcctg tatcagcgaa tagcgcgggg attggcgcca
     9361 accgatcagg cacgcgaatt cgcgcgccgg ctaaaggtcg ctctcaggga gctcgaatat
     9421 gggatcgagg aaattcgggc agcgcagggt gtttttacgt cacgaataac ggtcggcaac
     9481 ataccccatt ctggcaccca cctgctgtcg acgacgatca cccggtttct tgccatttat
     9541 ccgcgcgcat ctgttcacgt cgtcgatggc ccctacgagg tgctgctcga taccctgcgg
     9601 gccggcgatc tcgatctgct gttcggggta ctcagacgcc ccgcctgggc gaccgacgtc
     9661 agggaagaat ttttgttctc gaatccttac gccgttttcg tcggccgaca gcacccgctc
     9721 acccgcgttc ggaagataac gttatgagag ttggcccgct acggttggat tttgcccact
     9781 cgtggagctc cacgcaggga ggcattcgag cgtctttttg cgggctcggc gcattgcccg
     9841 actgtgcgta tcgaaaccac ctccttggag atctacaaat ccattcttgc gacaagcaac
     9901 atgatcgcgc tgctctcgac tgttgtggaa gcgcagctcg gcgaggcctt gacgatcctt
     9961 cctttcgact cttccgcgct ggagagaaat gatggtgtag caagccgagc agattggcag
    10021 ccgacccttt tgcatcaaca gtttcttgaa ttattgcgaa aggaagcgca gcagtatcaa
    10081 caagctcgaa ctgtaccggc cgacctcaat cgaagatcgc ttgcgcaagc cctgtaatca
    10141 gatcttcgat gtctctgagt ccattaaaac gtggtgcccc gctaagtgcg gcgtcaggat
    10201 cacgttatca aacgcaagca actcgtcatc agagcggccc ggttcttcat agatgcgccg
    10261 cggacgaacg gcgagctttt ccgagtgtgg catgcgctgt ctgccagtcg agcatggcgc
    10321 tgccgtcacc caactacgta cacggagacc aagcgatatc tgcgaaatgc cgcggtacca
    10381 cgccgttttc tgcggtcttc ccatgttagg tggtggagac cggactggct ggctggcgtg
    10441 agaggactcg aactccgaaa tgtcgtcgca aattatccct ttgaaaagtt gcgtagattt
    10501 gcggcgatcc agccggattc tggccacgga gactattcgc gtttgagctg cggcgttggg
    10561 gatacgcagc tcgaaaccta gttccag
//
LOCUS       DATLJT010001426         7587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2652834,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001426 DATLJT010000000
VERSION     DATLJT010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652834"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1344..2876
                     /locus_tag="VM910_32780"
     CDS             1344..2876
                     /locus_tag="VM910_32780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67316.1"
                     /translation="MREAEALRVRREKQQGLGRPIIAAKLRDHQMVAVGKRVHFSDKW
                     HTFHDFLRDHLFGLLGKEWGAVELAKPPEKRHRILRWFDQATEDARRTGTKIGDVYTA
                     PMSGATRAFVNLAYNIYLIAHHTEKASDAILKGYVDRLKSPRPDDFAGALFETYAAAA
                     FLKAGFTLAFENERDGSVSHVEFVALYPKTGKSFSVEVKARERGTGMAGAADVDDVKR
                     LRVANKLNKALGKKAAHTRVVMIEIDVPEVVTTLEGWPAAAMEQIRRNEQTDFPGGEK
                     KPSAYVFVTNHAFHSNLSAPDVGMQVLATGFHIPGFGPDAPHSSYKGVLETRVRHSEM
                     FALMKSMQTHYEIPSTFDGEMPQFAFQDSDGVPRLQFGRTYLVPTEDGRAVPGRLYDA
                     VVQEEQKQVVGCYQLATGVTVLARCPISDAELAAYRAYPDTFFGELRQPTRHAKTLVD
                     WCDFFYETYKDTPRDKLLEWMAGASDIERLQTLSQEELAIIICERWALNAFQTGNVKR
                     AS"
     gene            complement(3292..3579)
                     /locus_tag="VM910_32785"
     CDS             complement(3292..3579)
                     /locus_tag="VM910_32785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HVI67317.1"
                     /translation="MMKLKLSAISDDKPVRLTLELPATVHRDLVAYAEALGRQTGQKL
                     EPAKLVAPMLVRFMSTDRAFARLQRDKRATAQAPRPSITRPETASRDASER"
     gene            complement(3591..4775)
                     /locus_tag="VM910_32790"
     CDS             complement(3591..4775)
                     /locus_tag="VM910_32790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HVI67318.1"
                     /translation="MSEHGADLPSDLAAELRLRPERPPVMRLSRRVLMALVALATVSV
                     SGALIWALSQGQRKSTGATELYSTENKPTPDGLSALPRDYTGLAPNVPALGPPLLGDI
                     GHPVPNAQLGPASIDAEQQRVAQETEAARTSRLFATTNATERPVTPVSLPTASQPDQG
                     VTTQNSRAEVPPLDPDALLNMQDRKLAFLTAPVDRRTVSPDRLTNPPSPYVVQAGAVI
                     PAALITGIRSDLPGQVTAQVTENVYDSPTGRYLLIPQGSKLIGTYDSQISFGQDRVLL
                     VWTRLIMPNGRSIVLERQSGADTQGFAGLEDQVDYHWLRLFAAAALSTVLGVSSELGA
                     INNDNAIIQALREGSSNSLNQTGQQVVRRNLNIQPTITIRPGFPVRVIVNRDLVLAPY
                     RP"
     gene            complement(4772..5764)
                     /gene="trbG"
                     /locus_tag="VM910_32795"
     CDS             complement(4772..5764)
                     /gene="trbG"
                     /locus_tag="VM910_32795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HVI67319.1"
                     /translation="MRRHTLLLGLLLSITVAGCSTFKPPQISYDDARAEPAVLEAEPP
                     KLVHVVEVPKLLPLPGQLKPLAGRRPAPEPRDPKQRVNLANAAARVQPIRAGYINAVQ
                     VYPFAEGALYQVYAAPGDVTDIALENGEQLVGSGPVAAGDTVRWIIGDTESGAGSAKK
                     IHILVKPTRPDLVTNLVINTDRHTYHLELRSTERTYMASVSWIYPEDELIALHRQNAQ
                     ADAATPVATALDLNSLHFRYAIEGDNPPWRPVRAFDDGSKVYIEFPAGIGQGEMPPLF
                     IVGPEGKTSELVNYRVRGNHMIVDRLFAAAELRLGDGASEQRVRIVRSSGGLFW"
     gene            complement(5764..6444)
                     /gene="trbF"
                     /locus_tag="VM910_32800"
     CDS             complement(5764..6444)
                     /gene="trbF"
                     /locus_tag="VM910_32800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HVI67320.1"
                     /translation="MFRRPSVRYGRTLEPETPFQKAAQVWDERIGSARVQARNWRLIA
                     FSSLFLAAGLACGLLWQAARGTVTPWVVEIDKLGQAQAVAPAIGEYQPTDPQIAWHLA
                     RFIANVRSMSADPVIVRQNWLAAYDFVTDKGAVALNDYARANDPFSKIGKIEVAVEIS
                     SVIRASSDSFRVAWIERRYENASLAATEHWTAILTIVINTPHNAEHLRKNPLGIFVNA
                     INWSKELG"
     gene            complement(6447..7550)
                     /gene="trbL"
                     /locus_tag="VM910_32805"
     CDS             complement(6447..7550)
                     /gene="trbL"
                     /locus_tag="VM910_32805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HVI67321.1"
                     /translation="MNTGIIDRFTEVFTSYIDSGFGLLGGEVAFLASTLIVIDVTLAG
                     LFWAWAADEDVLAHLIKKTLYVGFFAFLIGNFNGLAKIIFNSFAGLGLKAAGSGLSPD
                     QFLQPGRIAQVGIDAGKPILQAAGQLMGFVSFFENFIQIFVLLVAWLVVVVAFFILAV
                     QLFVTLIEFKLTTLAGFVLVPFGLFGKTAFLAERVLGNVIASGVKVLVLAVIVGIGTT
                     LFGQFNQGFGGNQPSLEDALSLVLAAMSLLGLGIFGPGIATGLTAGAPQLGAGAAVGT
                     ALAAGGLGVAGAMGVRAGAAAVGRAAGAIAPARSVGAFAAAATQSPSGPPDWARRMRR
                     EQAISHGVSAAAHAVRSGDHGGGSTSVDLKEER"
BASE COUNT         1566 a         2208 c         2384 g         1429 t
ORIGIN      
        1 aaggattatt gctacaaacg tcacaccgtg cgtggccccc agaagggcgg tgcatatagc
       61 gagaaacttc gcctgtgcag ttgacgggat tatgtcgccg tagccgacgg tcgtgaaagt
      121 gataatctca tagtagaagt agtcaatcag aggtcggccc tgcgtaattg atggctctgg
      181 cgtttaggga agaagatcgt attcgatagc gaagactgac agaataccgc aaataaaaac
      241 ggaccacgcc agatacccaa tccaaacgta aaccggctct cgtcttgcgc gctgcaagaa
      301 agagaaaatg gcgcggatgg agaacccact ggtagcgctc gaaaggtcaa ctgacgatct
      361 cacttgcctt cctttcgccg aatctcgtaa gagccgccat tgaagcccgt acctcatggt
      421 ataggaacca ctcgattggc cgacctgccc gccgagtggt cgcggcagca ccggatgctc
      481 ggtctttcga accagtctcc aaaagaactg ctctctggtt ccgggaaacg gaattttgcc
      541 ggcagagaca aacgtgccgg aatggcccct aagacaaact tcgcatttga aggagacaaa
      601 tcacgcgcat gaaacgcggc tatttcgggt catcccgcca caaatgggga aatctctgtt
      661 tgcaccagac tgcgtggtgg ggctgggagg actcgaactt ccaaccaaac gattatcagt
      721 cgctagctct gaatattgag caaggggccc gcttgtggct agcgaagtgc ccaaggttac
      781 gtgtacgacc gctttgaaga acttctcccc aagctgtgag acgattctcg cagccaacgg
      841 atagcgaaaa gcaaaggctc gcgcggcccc tgggtggcat attcgtcgaa ctttgcagaa
      901 tgttgccagg aatgttgcca aattggcttt gcctctcatt ggagaagcac atctttggtc
      961 ctaaatgatt gattttattg gtgggcgcac aaggactcga accttggacc cgctgattaa
     1021 gagtaagctg ctcctggcgg cggacctatg accctcccgt gctgaccgtc aacgcggcga
     1081 gtcatgctcg gaaggacatg cacaaaagga aaggccttgt tttgccaaga atcggggcta
     1141 tggctgtatg tcagacgacg ttattgaaca aatgcatatt taaccgatcg ctgaatacgt
     1201 gatgcaatct catgcccaac attggaagaa atcagacttg cccgtgcggg agcggcaaaa
     1261 aatataaaag atgtcacggc gcctacccga tgcacgaatc gcgcttgcac gtccccgatt
     1321 cgcgggcggc cttcgctcag gggatgagag aagccgaggc gctgcgcgtc cggcgcgaaa
     1381 agcaacaggg attgggacgt ccaatcatcg ccgccaagct gcgcgatcac cagatggtgg
     1441 ccgtcgggaa gcgcgttcac ttttcggata aatggcacac gttccacgac ttcctccgcg
     1501 accacttatt tggccttctc ggcaaggaat ggggcgcagt cgaactggca aaaccgccgg
     1561 aaaagcgtca ccgcatactc cgctggttcg accaggcgac cgaggatgcg cggcggacgg
     1621 gcacgaagat cggcgatgtg tacacggcgc cgatgagcgg cgccacgcgc gccttcgtca
     1681 acctcgccta caacatctat ctcatcgccc atcacacgga gaaggccagc gacgcgatcc
     1741 tgaagggcta tgtcgatcgc ctcaagagtc cgcgtcccga cgacttcgcc ggagccctgt
     1801 tcgagaccta cgcggccgcc gcgtttctca aggcgggatt cacgctcgca ttcgagaacg
     1861 agagggacgg gtcggtgtct cacgtcgaat tcgtcgcgct ctatccaaag accggcaaga
     1921 gtttctccgt cgaggtgaaa gcccgggagc gcggaactgg catggccggc gccgcagatg
     1981 tcgatgacgt gaagcggctg cgggtcgcga acaagctcaa caaggcgctg ggcaaaaagg
     2041 ccgcgcacac gcgcgtggtg atgatcgaga tcgacgtgcc ggaggtggtc acgacgctcg
     2101 agggctggcc ggcagcggcg atggagcaga tacgccgcaa cgagcaaacc gactttcctg
     2161 gcggcgagaa gaagcctagc gcttacgtgt tcgtcaccaa tcacgcattt cacagcaatc
     2221 tctctgcgcc ggatgtcggc atgcaggtgc tggcgacggg atttcacatt cccggctttg
     2281 gacccgacgc gccgcattcg agttacaagg gcgttctcga aacgcgcgtc cgccatagcg
     2341 agatgttcgc tctcatgaag tcgatgcaaa cgcactacga aataccatcg accttcgatg
     2401 gcgagatgcc gcagttcgcg tttcaggatt ccgacggggt gccgcggctg caattcggcc
     2461 gcacatatct cgttcccacc gaggacggac gggcagttcc cggacggctc tacgatgccg
     2521 tcgtgcagga agagcaaaag caggtggtcg gctgctacca gctagcgaca ggcgtgaccg
     2581 tccttgcaag atgcccaatc tctgatgcgg aactggcggc ttatcgagcc tatccggaca
     2641 cgttcttcgg tgaattgcgc cagccaaccc ggcatgccaa gacgctggtc gattggtgcg
     2701 acttcttcta cgagacgtac aaagatacgc cgcgcgacaa gctgctcgaa tggatggccg
     2761 gggcatccga catcgagcga ctgcagacgc tgtcgcagga agaactcgcc atcatcatct
     2821 gcgagcgctg ggctcttaac gcgtttcaga cagggaatgt gaagcgcgca tcatgacgac
     2881 gccgaattga atatactagt gatgaacatg ttggatcgta atcatcggcg gcgtggctag
     2941 ctgatcgtct ttaggatctt tgccatgcag atgccatgca gattatccat ttctgcatgg
     3001 catacttctg gatacacgaa aagttattgt aaagtttaga cttctggcgc gcccgaagag
     3061 attcgaactc ctgaccccca gattcgtaat ttggtgcttc cccacacatc atcaacaacc
     3121 atctgattct atttgcatgt tcgagggcac cgggggaatc gatgccctcc atcacaaacc
     3181 agcttaaact cgaatctgtg gcacgcgcgc aagagcattc tcggcgcgtc tcggctccaa
     3241 tctcagatat gatcctgcac cgccagcctc gccccaagca aacggatgtg actaccgttc
     3301 tgaggcatct cgtgatgcgg tttcgggccg tgttatcgac ggccgaggcg cttgagccgt
     3361 tgctcgctta tcccgctgca agcgcgcgaa agctcggtcg gtcgacatga accgtacgag
     3421 catcggtgcc acgagtttgg ccggttcaag cttttgtccc gtctggcggc cgagagcttc
     3481 agcgtacgcg acaagatcgc gatgaaccgt agccggcagt tcaagggtga gcctcacagg
     3541 tttatcgtcg gagatcgcgg ataacttcag cttcatcatc tgatttcctc tcaaggtcga
     3601 tacggtgcca gcacgaggtc gcgattgacg atgacacgta ccgggaatcc tgggcgaatc
     3661 gtgatcgtcg gttgaatatt gagattgcgg cgcaccacct gctggccggt ttggttgagg
     3721 ctgttgctgc tgccttctcg cagcgcctgt atgatagcgt tatcgttgtt gatcgcgccg
     3781 agctcggaac taactcccag cacagtcgat aaagcggcgg ccgcaaacag ccgtagccaa
     3841 tggtagtcaa cctggtcctc gagccctgca aagccttggg tgtccgcacc ggattggcgc
     3901 tcaagaacta tcgagcggcc gttcggcatg atcagcctgg tccagaccag gagtacgcga
     3961 tcctggccga atgagatctg gctgtcgtag gtgccgatga gcttcgatcc ttgtgggatc
     4021 aggaggtacc ttccggtggg actgtcgtag acgttctcag tgacctgagc ggtgacctgt
     4081 ccgggaaggt cggaacggat gccggtgatg agcgctgccg gaatgaccgc gccggcttgc
     4141 acgacatagg gtgacggtgg gttcgtaagc cggtccggac ttaccgtcct gcggtcaact
     4201 ggcgcggtca ggaaggcaag cttccggtcc tgcatgttca ggagcgcgtc gggatcgagc
     4261 ggtggcacct cggcacggct gttttgtgtc gttactccct ggtcaggttg tgacgccgtg
     4321 ggcaatgaga ccggcgtcac gggacgctct gttgcattgg tcgtggcgaa cagacggctc
     4381 gtgcgggcgg cctctgtctc ttgtgcgacg cgctgctgct cagcgtcgat actcgccggc
     4441 cccaactgtg cattcggaac cggatggccg atatcgccca agagcggcgg gccgagagca
     4501 ggaacgttgg gggcaagacc tgtgtaatca cgtgggagcg cagagaggcc gtccggcgtc
     4561 ggcttgttct ccgtactgta gagctcagtc gcgccggtgg actttctctg tccttgggag
     4621 agcgcccaga ttagcgcgcc ggacacagaa actgttgcga gtgcgacgag cgccatcagc
     4681 accctgcgcg agaggcgcat caccggcggc cgctcgggcc gcaggcgcaa ctccgccgca
     4741 agatcgctgg gaaggtcggc cccgtgttcg ctcaccagaa cagccctcca ctgctgcgca
     4801 cgatgcgcac gcgctgttcc gaggcgccat cgccgagccg aagttcggcg gcggcaaaga
     4861 gccgatcgac gatcatatgg ttgccgcgga cgcggtaatt gacgagctct gaggtcttgc
     4921 cctctgggcc gacgataaag agcggcggca tttcgccctg gccgatgccg gcaggaaact
     4981 cgatgtacac tttgctcccg tcatcaaagg cacgaacggg tcgccacggc ggattgtcgc
     5041 cttcaatcgc gtaacgaaag tgcaggctgt tgaggtcgag cgcggttgcg acgggcgtcg
     5101 cagcgtcggc ctgggcgttc tgccgatgca gcgcaatgag ctcgtcctcc ggatagatcc
     5161 aggagaccga tgccatgtag gtcctttcgg tggagcgaag ctcgagatgg taggtgtgtc
     5221 ggtcggtgtt gatgacgagg ttggtgacaa gatctggccg ggtcggcttg acgaggatat
     5281 ggattttctt agcgctcccc gccccgctct cagtatcgcc gatgatccag cgcaccgtgt
     5341 cgccggcggc gaccggaccg gaaccgacga gctgctcgcc gttctccaga gcgatatcag
     5401 tgacgtctcc gggcgcggca tagacctgat agagtgcgcc ctcggcaaac ggatagacct
     5461 gcaccgcatt gatgtacccg gcccggatcg gctgcacacg ggccgccgca ttggcgaggt
     5521 taacgcgctg cttgggatcg cgcggctcgg gtgccgggcg ccggccggcg agcggcttca
     5581 gctggcctgg tagtggcaga agcttgggga cctcgacgac gtggaccagc ttcggcggtt
     5641 ctgcctccag cacagccggc tcggcacggg cgtcgtcata gctgatctgg ggcggcttga
     5701 aggtgctgca gccggccacg gtgatcgata acagcaggcc taataataat gtatggcggc
     5761 gcatcatccg agctccttcg accagttgat ggcgttgacg aagattccga gcgggttctt
     5821 gcgcaggtgc tcggcattgt gcggggtgtt gatcacgatg gtgaggatcg cggtccagtg
     5881 ctcggtcgcg gcgagacttg cgttttcata gcggcgttcg atccaggcga cacggaagct
     5941 gtcgctggag gcgcggatca cgctggaaat ctcgaccgcc acctcgatct tgccgatctt
     6001 gctgaaggga tcattggcgc gggcgtagtc gttgagcgcc accgcacctt tgtcggtgac
     6061 gaagtcgtag gcagcgagcc agttctggcg cacgatcacc ggatcggccg acatcgagcg
     6121 gacgttcgcg atgaagcgcg cgagatgcca ggcgatctgc ggatcggtgg gctggtattc
     6181 gccgattgcc ggcgccaccg cctgcgcctg gccgagcttg tcgatttcga cgacccacgg
     6241 agtgactgtg ccgcgggcgg cctgccagag gaggccgcag gcgaggccag ccgccagaaa
     6301 caagctgctg aaagcaatga gccgccagtt gcgcgcctgg acgcgggccg aaccgatgcg
     6361 ctcgtcccac acttgcgcgg ccttctgaaa cggcgtttcg ggctcgagcg tgcggccgta
     6421 acggaccgac ggacgtcgga acagcatcaa cgttcctcct taaggtcaac agatgtcgag
     6481 ccgccgccgt gatcgccgga gcgcaccgcg tgagcggctg cggacacccc gtgcgagatc
     6541 gcctgctcgc ggcgcatgcg gcgagcccag tcgggcgggc cgctcgggct ctgggtcgct
     6601 gcggccgcga acgcgccaac ggaccgcgcc ggcgcgatcg ctccggcggc gcggccaacg
     6661 gcggcagcgc cggcgcgtac gcccatcgct ccggcgacgc caagccctcc cgccgccagc
     6721 gccgtgccga cagcggcgcc cgcgccaagc tgcggcgcgc ctgccgtcaa gccggtggcg
     6781 atgcccggtc cgaatatgcc gaggccgagt agcgacatcg cggccagcac tagcgagagc
     6841 gcatcttcaa gtgacggctg attgccgccg aatccttgat tgaactggcc aaacagcgtg
     6901 gtgccgatgc cgacgatgac ggcaagcacc agcactttga cgcccgatgc gatgacgttg
     6961 ccgagcacgc gctcagcgag aaatgccgtc ttgccgaaga ggccgaacgg cacgagcacg
     7021 aagcctgcga gcgtcgtcag cttgaactcg atcagggtga cgaagagctg caccgccagg
     7081 atgaagaagg cgaccaccac cacgagccag gcgacgagca gcacgaagat ctggatgaag
     7141 ttctcgaaga aggagacaaa gcccatcagc tggcctgcgg cctgcaggat cggcttgccg
     7201 gcatcgatgc cgacctgtgc gattcgtccg ggctgcagga attgatccgg cgacaggcct
     7261 gatccagccg ccttgaggcc gaggccggca aaggaattga agatgatctt ggcaagacca
     7321 ttgaagttgc cgatcaggaa agcgaagaac ccgacgtaca gcgtcttttt gataagatgc
     7381 gcgaggacat cctcatcggc ggcccaggcc cagaacaggc ctgccagcgt gacgtcgatc
     7441 acaatcaggg tcgaagccag aaacgcgacc tcgccaccga gaaggccgaa gcccgagtcg
     7501 atgtaactcg tgaagacctc ggtgaaacgg tcgatgatgc cggtgttcat tgcggctgca
     7561 ctgtcctgtc gctcggcgag gaaaggc
//
LOCUS       DATLJT010001427         5306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_584732,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001427 DATLJT010000000
VERSION     DATLJT010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584732"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="VM910_32810"
     CDS             complement(<1..151)
                     /locus_tag="VM910_32810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HVI67322.1"
                     /translation="MAIFLQFVVGLIFGFGLMLSGMSNPAKALNFLDLGGIASGSWDP
                     SLTFVM"
     gene            complement(157..587)
                     /locus_tag="VM910_32815"
                     /pseudo
     CDS             complement(157..587)
                     /locus_tag="VM910_32815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
     gene            868..2070
                     /locus_tag="VM910_32820"
     CDS             868..2070
                     /locus_tag="VM910_32820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67323.1"
                     /translation="MVEIADTAMVRRASIKDATARRAQLEPAGAEPLTPLTAVEPSEW
                     QALADSAIEPNAYYLPGWELAVNASVPGRTGALALAARSDIRLIGLLPVVPMARALKI
                     PLPALVSAHPYGTLCTPLLTRMGAEDAAARLMEQAREAGARALLLREVALDGAAMKAF
                     KAVLRGEDIRPRVLHWHLRACLNATRSADELLQDALSGKKLKDLRRQRHRLAEHGAVG
                     FDVARTPRDVAAAIETFLQLEASGWKGQRGTALMQVEGDATFIRSATAALAKTGQCEI
                     VTLRAGKDPVASGIVLRHQGRAFFFKLGVDERFAKYSPGVQLTLELTRHLCADSKITS
                     ADSTASPDHPMINPIWRGRFAIGDVLIPLRRNDPVVTLIHAALVTRQSALAWARWGLR
                     TIRGRRKR"
     gene            complement(2085..2723)
                     /locus_tag="VM910_32825"
     CDS             complement(2085..2723)
                     /locus_tag="VM910_32825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVI67324.1"
                     /translation="MIYELRTYTVRPGTIGDMVKAASTVSRDIRGDRFGKLEGYWWTE
                     IGPLNQVMHMWSYSDFNERATLRAELAKNSRWAGEYIPLIRPLLMRQEVRLLNAIKPP
                     VAPASTGNVYELRNYRAKPLGAVRQWLEAFTAALPAREKYSKIVGLWQTEAGQPNEVC
                     HIWAYSSLNARAEARANAQKDPVWQEFLGKGPAFLEEMHSTIMLPASHSPLR"
     gene            complement(2804..3469)
                     /locus_tag="VM910_32830"
     CDS             complement(2804..3469)
                     /locus_tag="VM910_32830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HVI67325.1"
                     /translation="MSTLKPLKIDIVSDVVCPWCYIGKKRIENALALVPDVRVEVKFR
                     PFFLNPWVPREGISREDYLTTKFGSVDAYKGIAGRVVAAAGEEGLTYRPDLVKRQPNT
                     IDCHRLIHWAEAIGKAPEMKQRLMELYFRDGGDLTDVDVLVRAAADVGLDADDVRKRL
                     ATDEDIDLISGQAQEASDKGISGVPTFVFAQKYAISGAQAPELLARAIRQVSAEVNAQ
                     AAE"
     gene            complement(3616..4440)
                     /locus_tag="VM910_32835"
     CDS             complement(3616..4440)
                     /locus_tag="VM910_32835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI67326.1"
                     /translation="MNRHTPTAQTERARPIHIGDHLREWRQRRHLSQLDLAGDAEISA
                     RHLSFVETGRAAPSREMVLKLAERLDVPLRERNVLLVAAGYAPAFPQRTLDDPALKAA
                     RQAIDLVLKAHEPNPALAVDRHWNLVSANRMVAPLLEGVPQRLLGQPFNVLRLSFHPE
                     GLAPHTVNLAEWCAHLLERLHRQCEATADPELLKLYEDLKAFPIPARRAPLPTDNVAI
                     PFKMRHEGDVLSFFSTTMVFGTPVDITLSELALETFFPADDFTAERMRKIARSLDN"
     gene            complement(4605..>5306)
                     /locus_tag="VM910_32840"
     CDS             complement(4605..>5306)
                     /locus_tag="VM910_32840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HVI67327.1"
                     /translation="LDCRKKKDDAIENKYLFELNNRWTIDGSVRENIARYINHACKPN
                     AESDVKPRKRKVFIRAIKNIEPGEEINYDYGTDYFKAYLKPIGCKCGACEKKRRKKRA
                     EARAEREREKKRIERKAKRLAEKLARANAKLKKLNGKLKGANGKDASRKPANGKYANG
                     HNRAWRRDRSRRTTARRDLMARSAPAEQPPQQQERAQALAGAGFHGLHLRDHIDAGAL
                     DQQFGHLQRTREKQG"
BASE COUNT          958 a         1732 c         1642 g          974 t
ORIGIN      
        1 ccatgacgaa ggtgagactc ggatcccagc ttccggaagc gatcccgccg aggtcgagaa
       61 agttgagggc ctttgcggga ttcgacatgc ccgaaagcat gagcccgaaa ccgaagatca
      121 gtccgaccac aaattgcaga aagatcgcca tgatcgctaa cccagatgtc gcgcgacgaa
      181 gacggtcgca aagcccgttg ccatgaaggt gatcgtggca acgagcgcgc gcaccgatag
      241 tcgtgagagc ccgcacacac acaatggccg gaggtgcaac cgttgcccca caccgaaccg
      301 aagccgacca gaaatcctgc aagcaccagc actgccgcgc tggcctcgat cgtctgtgcc
      361 gggagccgtc cggtggcaat caggcaaacg aggggtgcgg cgaccaggcc ggcaacgaag
      421 gcgagccgcc cggcaaattc gcgatcctcg tagggcggca gcaggcgtgc ggcgatgccg
      481 ctgacgccgg caatgcgccc ggtgacggac atcaacatga cggcggcagc tccgatcagc
      541 gcgcctccga gggaggatgc gaccggcgtg aaggaagtga gcgtcattca acaaacctct
      601 ggtttcgcgt caacgtcatg gcggtttggc gcctgctcct cattgcggta tcgcaaagaa
      661 ccctgcatga aaccggcgtc cgcatccgaa attatatcat gagcgcccct gccgaatgcg
      721 gatgctgaat tttcccttaa tacgagccgt caaattgcac ggcgacgcca acagtatttt
      781 ccgatctttc cgctaggttg acccggcgaa ggcgaagatc gcggcctcag acaacgcgac
      841 gcgccctctc gcacggggac acagggcgtg gtcgagatcg ccgatactgc gatggtccgt
      901 cgcgcatcca tcaaggatgc cacggcgcgg cgcgcccagc tcgagccggc cggcgccgag
      961 ccgctgacgc cgcttacggc cgtcgagccg agcgaatggc aggcgctcgc cgattccgca
     1021 atcgagccga acgcttatta tcttcccggc tgggaattgg cggtgaatgc ctcggtgccc
     1081 ggccgcaccg gcgctctcgc cctcgctgcg cgcagcgata tccgcctgat cgggctcttg
     1141 cccgtggtgc cgatggcgcg cgccctgaaa attccgctgc cggcgctggt cagcgcccat
     1201 ccctacggca cgctttgcac gccgctgctc acccgcatgg gagccgaaga tgccgcggcg
     1261 cgcttgatgg aacaggcgcg cgaagccggc gcccgcgcgc tgttgctgcg cgaggtcgca
     1321 ctcgacgggg ccgccatgaa ggccttcaaa gcagtgctgc gcggcgagga cattcgtccg
     1381 cgcgtgctgc actggcacct gcgcgcctgc ctcaacgcga cgcgcagcgc cgacgaactg
     1441 ctccaggatg cgctgagcgg caaaaagctg aaggatctgc gccggcagcg ccaccggctg
     1501 gccgaacatg gtgccgtcgg ctttgacgtc gcgcgcacgc cgcgggacgt cgcagccgcg
     1561 atcgaaacct ttttgcagct cgaagcgagc ggctggaaag gccaacgcgg caccgcgctg
     1621 atgcaggtcg agggcgacgc aacgttcatc cgtagcgcca cggcggcgct cgccaaaacg
     1681 ggccagtgcg agatcgtgac cctgcgcgcg ggcaaggatc ccgtcgcctc gggaatcgtg
     1741 ctgcggcatc aaggccgcgc tttcttcttc aagctcggcg tcgacgagcg ttttgccaaa
     1801 tactcaccgg gcgtgcagct cacgctggaa ttgacccggc atctctgcgc cgactcgaag
     1861 atcacctcgg ccgactccac cgcaagtccc gatcatccca tgatcaatcc gatctggcgc
     1921 gggcgctttg caatcggcga cgtgctcatc ccgcttcggc ggaacgaccc ggttgtcacg
     1981 ctgattcatg cagcgctggt cacgcgccaa tcggccctcg catgggcacg ttggggcttg
     2041 cgcacgatcc gcggacggcg gaagcgctaa acctcctgcc agtcttatcg caagggcgag
     2101 tgagacgccg gcagcatgat ggtcgagtgc atctcctcga gaaacgcggg acccttgcca
     2161 aggaactcct gccacaccgg atccttctgc gcgttcgcac gtgcctcggc ccgcgcgttg
     2221 aggctggaat aggcccaaat atggcagacc tcgttcggct ggccagcctc ggtctgccag
     2281 aggccgacga tcttcgaata tttctcgcgc gccggcagcg ccgccgtgaa cgcctccagc
     2341 cattgcctca ccgcgccgag cggcttggcg cggtaattgc ggagctcata gacgttgccg
     2401 gtcgaggccg gggccactgg cggcttgatg gcgttcagga gacgcacctc ctgacgcatc
     2461 agcagcggac ggatcaatgg aatgtactcg ccggcccagc gcgagttctt ggcgagttcg
     2521 gcgcgcaacg tcgcgcgctc gttgaaatcg ctgtagctcc acatatgcat gacctgattg
     2581 agagggccaa tctcggtcca ccagtagcct tcgagcttgc cgaaacgatc gccgcggatg
     2641 tcacgcgaca ccgtgctcgc cgccttgacc atgtcgccga tggtcccagg cctcaccgtg
     2701 taggtccgca actcatagat catgatattc cccctttctt cttttcgatc tgaagacctg
     2761 tcagacgata gagcgaaacg ccatgcccgt cggcagcagc ggcctattcg gccgcctgcg
     2821 cgttcacctc tgccgaaacc tggcggatgg cgcgggcgag caactcgggt gcctgcgcgc
     2881 cggagatcgc atatttctgc gcgaagacga aagtcggcac gccggaaatg cccttgtcgg
     2941 atgcctcctg ggcctgcccg gaaatcagat caatgtcctc gtcggtggcg agccgcttgc
     3001 gcacgtcgtc agcatcaagc ccgacatcgg ccgccgcccg cacaagaacg tcgacatcgg
     3061 tcagatcgcc gccgtcgcgg aaatagagct ccatcagccg ctgcttcatc tcgggtgcct
     3121 tgccgatcgc ctcggcccag tggatcagcc gatggcaatc aatggtgttg ggctggcgct
     3181 tgacgaggtc cggccgatag gtcagcccct cctcgcccgc tgcggccacg acgcggccgg
     3241 caatgccctt gtaggcatcg accgaaccga acttggtggt gaggtaatct tcccggctga
     3301 tgccctcgcg cggcacccag ggattgagaa agaacggcct gaacttcacc tcgacccgaa
     3361 cgtccggcac gagcgccagc gcgttctcga tccgcttctt gccgatatag caccacgggc
     3421 acaccacgtc ggagacgatg tcgatcttca gcggctttag ggtgctcatc ggaccatcct
     3481 cgtagattga tggtctcagg ataagcccaa ccgggggcga cacaagcgcc agtgtggcag
     3541 ttcagaagtc cgcatcactc atggcccggc gagttcgcac tcccggattt ctgagccaaa
     3601 gccacacgag atcaattagt tgtcgagcga gcgggcaatc ttccgcatcc gctcggccgt
     3661 gaaatcgtcg gccggaaaga atgtttcgag cgccagctcc gacagcgtga tatcgaccgg
     3721 cgttccaaac accatagtgg tcgagaagaa gctcaagaca tcgccctcat gacgcatctt
     3781 gaaggggatc gcgacattgt cggtcgggag cggcgcgcgc cgcgccggaa tcggaaacgc
     3841 cttgaggtcc tcataaagct tcaggagttc cggatccgcg gtggcctcgc attgccggtg
     3901 cagccgctcc aggagatgcg cgcaccattc ggcaagattg accgtgtgcg gcgcgagccc
     3961 ttcgggatga aagctcaggc gcaacacatt gaagggctgg ccgagcagcc gctgcggcac
     4021 gccctcgagc agcggcgcca ccatgcggtt cgccgacacc aggttccagt gccggtcgac
     4081 cgctagcgcc ggattgggct catgcgcctt caggacgagg tcgatggcct gccgcgcggc
     4141 cttgagggcg ggatcgtcca gcgtccgctg cgggaaggcc ggcgcatacc ccgcggcgac
     4201 cagcagcacg ttgcgttcgc gcaggggcac gtcgagacgc tcggcgagtt tcaggaccat
     4261 ctcgcgcgac ggcgcggcac ggccggtttc gacaaaactc aagtgccgcg ccgagatttc
     4321 ggcatcgccc gccagatcga gctggctcaa atgacggcgc tgccgccatt cgcgcagatg
     4381 atcgccgata tgaatgggcc tcgcacgttc ggtctgcgcg gtgggcgtat gtctgttcat
     4441 ggcacaaaag ctagcacggg gatttcgccc catccattac ctctgaggta atcgattagc
     4501 ctcgcccacg gccgcatctc tccaggcaaa ggagaggacc gatgcgaact ttctggcgct
     4561 attttcccac ggcaacgagc ctgatgccgc ggcttctcag cctgtcaacc ctgcttctcg
     4621 cgcgttcgct gcagatgccc gaactgctga tcgagagcgc cggcatctat gtggtcgcgc
     4681 aaatgcaggc cgtggaaacc agcgccggca agggcctgtg cccgctcttg ctgttgcggc
     4741 ggctgttcgg ccggcgctga tcgcgccatg agatcccgac gagctgtcgt cctgcgcgac
     4801 cggtcacgcc gccacgcgcg attgtggccg ttggcgtatt tgccgttggc gggcttgcgg
     4861 ctggcgtcct tgccattcgc acccttcaat ttgccgttca gcttcttcaa cttcgcattg
     4921 gctctggcga gcttttcggc gagccgcttt gccttccgtt caatcctttt cttctcgcgc
     4981 tcgcgttccg cgcgcgcttc ggcccgcttc ttcctgcgct tcttctcgca ggcgccgcat
     5041 ttgcagccga tcggcttgag gtaggccttg aagtaatcgg tgccgtagtc gtagttgatt
     5101 tcctcgcccg gctcgatgtt cttgatcgca cggatgaaaa ccttgcgctt gcgcggtttg
     5161 acatcgcttt cggcgttcgg cttgcaggcg tgattgatgt agcgggcgat gttctcgcgc
     5221 accgaaccgt cgatggtcca gcggttgttg agttcgaaca gatatttgtt ctcgatcgcg
     5281 tcatccttct tctttcggca atcgag
//
LOCUS       DATLJT010001428         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1733781,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001428 DATLJT010000000
VERSION     DATLJT010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733781"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VM910_32845"
     CDS             complement(<1..1225)
                     /locus_tag="VM910_32845"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVI67328.1"
                     /translation="MAASSSIEIARHTLLSCGLILAVGTLAAFVAQRIRIPDVAIFLL
                     AGIALGPHGLSLVNITADSALNQIILLFGASYILFDGGAALRLNVLKRVWITILVIAT
                     LGVLITAAIVALAASYVFALPLGVAALLGAVLASTDPATLMPIFRQVKIKARVAQTVI
                     AESAFNDAMSAIITFAALALATGDGHVSFLQSLFDLLRQSALGIVAGLALGYLASLLI
                     AHERWAFLAEYAPVVTLVAVIAAYFAASDLQASGFMAVFVFGIVLGNQETFGFQMKVG
                     EAQKLAEFVLTTSFIMRLFIFILLGAQVDFAEVGRHWLGAVAVATVLMLVARPITVFL
                     CALPDRRARWRLPELLFMCWTRETGVIPAALAGLLLSSQAPGAHVIASVTFVAILMTI
                     LIQAPTTEWVARKLGL"
     gene            complement(1329..2270)
                     /locus_tag="VM910_32850"
     CDS             complement(1329..2270)
                     /locus_tag="VM910_32850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67329.1"
                     /translation="MAVVLDPDAVAVYKAFQEAGRPAYETLTPPEAREYYMQARLVAN
                     PEPPELLSAEPLAIPAPHGRIPARIYTPKKLRKADGLAPGLVFFHGGGWVIGNLETHD
                     VVCRKLAHEGEMIVMSVDYRLAPEHRFPAAVEDAVTATKWVAANAKGLGIDASRLVVG
                     GDSAGGNLAAVVSLHARENGPKLAGQLLVYPATDFSRKHPSHSEPETSILLTHTVVTW
                     FMNNYLGDADINDWRASPARARTLADLPPAYVLTAGADPLRDEGDEYAARLKEAGVAV
                     TYRHFPGQFHGFFTMGKLLNQANIAVGEIATWLKGLD"
     gene            complement(2310..>2541)
                     /locus_tag="VM910_32855"
     CDS             complement(2310..>2541)
                     /locus_tag="VM910_32855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67330.1"
                     /translation="NCVCPGLIDSRMLSAIFQGRFGGNAPPPMEKIVERIPARRLGLA
                     SEVASIVAFLASDEASYVSGSAYTVDGGRTAA"
BASE COUNT          492 a          795 c          846 g          408 t
ORIGIN      
        1 gcagcccgag cttgcgagcc acccactcgg tggtcggcgc ctggatcagg atcgtcatca
       61 ggatcgcgac aaaggtgacg gaggcgatga cgtgcgcgcc gggcgcctgg ctgctgagca
      121 ggaggcccgc gagcgccgcc gggatcacgc ccgtctcccg cgtccagcac atgaacagaa
      181 gttcgggcaa gcgccagcgc gcgcgccgat cgggcaaggc gcagagaaat acggtaatcg
      241 gtcgcgcgac cagcatcagc acggttgcga ccgcgactgc accgagccaa tgcctcccga
      301 cttcggcaaa atcgacctgg gcaccgagca ggatgaagat gaagagacgc atgatgaacg
      361 aggtggtcag cacgaattcg gcgagcttct gcgcctcgcc taccttcatc tggaagccga
      421 acgtctcctg attgccgagc acgatgccga acacgaacac cgccatgaag cctgatgcct
      481 gcaggtcgct cgccgcgaaa taggcggcga tcacggcgac cagggttacg acgggtgcat
      541 attcggcgag aaacgcccat cgctcgtgcg caatcaggag tgacgcaaga taaccgagcg
      601 cgaggcctgc gacgatgccg agcgcggatt gcctcaacag atcgaacagg ctctgcaaaa
      661 acgagacgtg gccgtcgccg gttgcgagcg ccagcgccgc gaacgtgatg atcgcgctca
      721 tcgcatcgtt gaacgcggat tcggcgatca cggtctgcgc gacgcgcgcc ttgatcttga
      781 cctggcggaa gatcggcatc agcgtagccg ggtcggtcga agccaacacc gcaccgagca
      841 gggccgcaac gccgagcggc agggcgaaga cataggacgc cgcaagcgcg acgatcgcgg
      901 cggtgatcag cacgccgagc gttgcgatca ccaggatggt gatccacacc cgtttcagca
      961 cgttgaggcg cagtgcggcg ccaccgtcga acaggatgta gcttgcgccg aacagcagga
     1021 tgatctggtt cagcgcggag tcggccgtga tgttgacaag ggaaagcccg tgcggcccga
     1081 gcgcgatccc tgccagcagg aagatcgcga cgtcgggaat ccgaatccgc tgcgcgacga
     1141 acgccgccag cgtgccgacc gcaaggatca gcccgcagga gagcagcgtg tgcctggcga
     1201 tttcgattga gcttgatgct gccaacgcgc gatcagtcct gaatgtcgat gatccggcgt
     1261 tttccggatc ttccccgagt cgaccgggcc aaggtaaccc ggatcgtagc ttccgatcgc
     1321 gtcgcgtttc aatccagtcc tttcaaccag gtcgcgatct cgcccacggc gatgttggcc
     1381 tggttcaaga gcttgcccat cgtgaagaag ccgtggaatt ggccgggaaa atgccggtag
     1441 gtcacggcaa cgcccgcttc cttcagccgc gcggcatatt cgtcgccttc gtcacgcaag
     1501 ggatcggcgc cggccgtaag cacataggcc ggcggcaggt cggcgagcgt cctggcgcgc
     1561 gccggcgaag cgcgccagtc gttgatgtcg gcatcgccta gataattgtt catgaaccag
     1621 gtgacgaccg tatgcgtcag taggatcgag gtttcgggtt cgctgtgcga cggatgcttt
     1681 cgggaaaaat cggtcgcggg gtaaaccagg agctggcccg cgagcttcgg cccgttttcg
     1741 cgcgcgtgga gcgagactac ggccgcgaga ttgccgccgg cgctgtcgcc gccgaccaca
     1801 aggcgtgacg catcgatgcc gagccctttc gcattggcgg cgacccattt ggtggcggtg
     1861 acggcgtcct cgacggccgc tggaaatctg tgttccggcg caagccgata gtcgaccgac
     1921 atcacgatca tctcgccctc atgggcaagc ttgcggcaca ccacgtcatg ggtttcgaga
     1981 ttgccgatca cccagccgcc gccgtggaaa aacacgagcc cgggtgcaag cccgtccgcc
     2041 ttccggagtt tcttcggcgt gtagatgcgc gcagggatcc tgccgtgcgg cgcggggatc
     2101 gcaagcggct ccgccgaaag cagctcgggc ggctccggat tggcgaccag gcgcgcctgc
     2161 atgtagtact cacgcgcttc gggcggcgtc agcgtctcgt aggcgggacg gccggcttcc
     2221 tggaacgcct tgtagacggc gacggcatcg ggatcgagaa caacggccat gctggaaaac
     2281 cttctgtcgt ttgaacgttg cacgattgct cacgccgcgg tgcgcccgcc gtcgaccgtg
     2341 taggcggagc ctgagacgta gctcgcttca tcggaggcca ggaacgccac aatggaggca
     2401 acctcggagg cgagtccgag acgccgcgcc ggaatccgct cgacgatctt ctccatcggc
     2461 ggcggcgcgt tgccgccaaa gcggccctgg aagatcgcgc tcagcatgcg gctgtcgatc
     2521 aggccgggac agacgcaatt g
//
LOCUS       DATLJT010001429         1517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2880887,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001429 DATLJT010000000
VERSION     DATLJT010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2880887"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..813
                     /locus_tag="VM910_32860"
     CDS             199..813
                     /locus_tag="VM910_32860"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67331.1"
                     /translation="MMTLYYAPGACSMAAHIVLEESGEKYQPQKVDLAGGEQRTDAYL
                     KINPQGRVPALRLDNGEPLAENTAILPYLGKRFGLWPSEALAEAKALSLIGFFAASVH
                     PAHAHVGRPERYAADPSAFPTLKETGLKTFHNYLKQIDGKLAGREWFGDRYSVVDPYG
                     FVFYTWGVRRELPMAELKNYTAFKDRMLKRPAVGRVVEDEKIKV"
     gene            complement(1298..>1517)
                     /locus_tag="VM910_32865"
     CDS             complement(1298..>1517)
                     /locus_tag="VM910_32865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67332.1"
                     /translation="LQRVTGTSVQEIDKLITELQTLRDTLQSEAARVQREIVEYATLS
                     QAALQSTKIIAESLTHWKKVPDAPSLRD"
BASE COUNT          241 a          499 c          481 g          296 t
ORIGIN      
        1 ggtgttggct gggaacgatg aattcacgct taaacgcgtt accacccgcc gcgtcgctgc
       61 gtgtgccgca aggactgcgt gcgcgatgag cgtgtcggcg ttcgcggtcg cgctggcatc
      121 gggcgaggcg gcggcctatc atcgtcgccg agctttctct cacgacacaa tcgctgcatc
      181 cacaaggagg gttttgcgat gatgacgttg tattacgccc cgggggcgtg ctcgatggcc
      241 gcccacatcg tgctggaaga gagcggcgaa aaatatcagc cgcagaaggt ggacctggcc
      301 ggcggcgagc agcgcacgga cgcctacctc aagatcaatc cgcaggggcg cgtgcccgcg
      361 ctgcggctcg acaacggcga gcccctggcc gagaacaccg ccattctgcc ttatctcggc
      421 aaacgcttcg gcctgtggcc gagcgaggcg ttggccgagg ccaaggcgct ctcgctgatc
      481 ggcttctttg ccgcgagcgt gcatcccgcg cacgcccatg tcggccggcc cgagcgctac
      541 gcggccgacc cgtcggcatt tcccacgctc aaggagacgg gcctcaagac cttccacaac
      601 tacttgaagc agatcgacgg caagctcgcc ggccgcgaat ggttcggcga ccgatattcc
      661 gtggtcgatc cctatggctt cgtcttctac acctggggcg tgcgccgcga gctgccgatg
      721 gcggagctca agaattacac cgcgttcaag gaccgcatgc tcaagcgtcc cgccgtcggg
      781 cgcgtcgtcg aggacgaaaa gatcaaggtc tgatcgcgcc gcgcccgtcc gctagagcat
      841 ctttgtttgt gcttgaagca aatgacgccg cgcactcggt gcactcccct cccccttgtg
      901 gggaggggtg ggggtggggg tccttcatcg gatccccgca cgacccccac cccggcgctt
      961 cgcgccgacc ctccccacaa gggggagggt aggaccgagt ttgccgcgcg cgctgattcc
     1021 atttcatgaa cgcgctctcc ggcgctgtgt gagctgcttc acgctcgact cggcaaatgc
     1081 cttctatcgt gatgcggtca ccgagcgatc ggtgatggcg gtaggtggtc gcctaccgcc
     1141 gcccctcttg ggcgtttcct ccctcgactc ggccccgttg gcttttgccg gcggggcctt
     1201 tttcatcgcg agtggttcgc gatcgcgggc tggtctttgc aaaaaaaaat gcgccggccg
     1261 aagccggcgc gacctggcat gtggtgacta tgcgccctca gtcccgcaga ctcggggcgt
     1321 cgggaacctt tttccaatgg gtcaggcttt ccgcgatgat cttggtcgac tgcaaggccg
     1381 cctggctcaa ggtcgcgtac tcgacgatct cgcgttgcac gcgcgcggcc tcgctctgca
     1441 gcgtatcacg caacgtctgc agctcggtga tgagcttgtc gatttcctgc accgacgtcc
     1501 ctgttacccg ctgcaag
//
LOCUS       DATLJT010001430         6607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3339811,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001430 DATLJT010000000
VERSION     DATLJT010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3339811"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..459
                     /locus_tag="VM910_32870"
     CDS             289..459
                     /locus_tag="VM910_32870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67333.1"
                     /translation="MPDVDAGVYAQITESRKAQLNIENIFNKGYWASADGNNNISPGQ
                     PRTLRFKVTARL"
     gene            complement(491..2005)
                     /locus_tag="VM910_32875"
     CDS             complement(491..2005)
                     /locus_tag="VM910_32875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67334.1"
                     /translation="MAAEFDGSTVTGTLNMEMLHVDKPLFMRNGQFGEVVLRDAHIGA
                     LELDGSKVSRTLNMEKLRVDSSVFMRHGQFSDVVLRNDRLGGPLEFDGATVTGTLNMN
                     MLNVDSSLFMRDGQFGEVVLRDAHIGALELDGSRVSRTLDMEKLRVDSSVYMRHGQFG
                     EVVLRSARISGTLELDGSKVSRTLNMDKLRVDSSLFLRNDAKFADVNLTSTRVGGQLN
                     LVHAKVGGNLQCYSLVVDEDGLLYDAQFSGRIDCQFAKFRSLYLDNSTFGGDVDLSSA
                     QLSGELALGEAVPQHSPQWSPGKTLILRNARAETIPILTDAWPAQVDVNGFTYKALRR
                     DIAVDHHRTPECPSDLFRCWFGKQQSFSPHAYEQLALAFQNQGHADDARAIRYDGRER
                     EVSESGGLRYAWLTTLNWAIGYGHHIELAMIWTLVLVILGVFVLGVSGEGPKNGMPVG
                     LSYSFDMLLPIIKLRDAHYKIDLMGWPRYYFYVHKIAGYVLASFLIVGISGLTK"
     gene            complement(1995..2738)
                     /locus_tag="VM910_32880"
     CDS             complement(1995..2738)
                     /locus_tag="VM910_32880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67335.1"
                     /translation="MLIDVAEERVGTSCFFLISLVLLGASFEALGEGRPAAPSADGKC
                     PVAADTQRWTKQEIFVWDRVCSGAIADFNEGTAYGGNLDPNGPEGLPESRILSSRFLE
                     TILLEDKYRSALPHRGVRIIGARFKERIDLVNAELKHELWLNRCLLEQGVDFSGAKSS
                     NLLSFDGSKVTGEFTMNGSRIDDSVFMPDARFADVAMGGTRIGRTLEFDGSTVTGTLN
                     MEMLHVNSFLFMRDGQFREVMLRNGRVGG"
     gene            complement(3300..4181)
                     /locus_tag="VM910_32885"
     CDS             complement(3300..4181)
                     /locus_tag="VM910_32885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67336.1"
                     /translation="MVVPFPAGGPTDTVGRIVAERMRLSLGQPIIIENVTGAGGTIGV
                     GRVVRAIPDGYTLSVGFLGTHVLNGAIYPLQYDVLNDFEPIALLASNPQLIVAKNATP
                     AKSLNELIAWLKANPGKASQGTAGVGSPAHVSGAYFQKETGTRFQFVPYRGAAPAMQD
                     LIAGQIDLMFDQALNALPHVRASKIKAYAVTAAARLASAPEIPTVDEVGLPRFYISIW
                     SGMWAPKGTPKNAVAKLNAAVVDALAHPTVRQHLADLGQEVPPRDQQTPEMLRAFQKA
                     EIEKWWPIIKAANIRAE"
     gene            complement(4533..5963)
                     /locus_tag="VM910_32890"
     CDS             complement(4533..5963)
                     /locus_tag="VM910_32890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67337.1"
                     /translation="MSTTDENNFIAFSHHGGDWPSGAVFWAFPGEATYAPDQGFLASG
                     IGNDPHVKIGGQFGGWFYGVRGSGGLSVDADAQHEVWPPVIGKSEFKDGPEVNDYSNF
                     AGVLGTGIFVTGVAGTSVNNFGVYGQTEEDADASIPQNLSAGVVGAATTGSGVVGWST
                     TWNGVEGWAYQGTAVLGVSDSGYGVQGASAWQPGVLGWSTNDTGVTGGSGPEGTEGPP
                     LPDGQPTIAGVVGTSDARPGVIGTSNTAAGVFGFSNIIGVAGQSTKRAPAGYAGYFLG
                     DLSVTGQIFAGVKDAIVPFPDGSKRVLHCMESPEHWFEDFGTAKLKRGRAVVRLDADF
                     AKAIKRSDYRVFPAPEGDCRGLYVRRKSAASFEVRELMGGKSNIAFSYRIVGRRKDIK
                     ERRRFAKLDTRLPLAAVAARRGRKAEPTAAGLRAFSARAEKDARARRPKRVEAARRAF
                     VVRAEKEARARPPIVPKLQPPRPERK"
BASE COUNT         1306 a         2077 c         1873 g         1351 t
ORIGIN      
        1 cgcaccgcct ccgggcggga agccaacaac gatgcgcacc ggccgcgtcg gataggtctg
       61 cgcccaagca atatgcgaca cggccggcag cgcggaagcg ccggcggcca gatgcagaaa
      121 tttccggcgg ggaagtttca ttttgaacct ctcttcacgt cgttcgatta cctcgtcatc
      181 ccaatctggc tccgcgtctg cgtcaattca ctcctaaaaa ttctcgtcga tgatcttggc
      241 tagtcgaatt cccgcgctca acagctgttg ctgcaccacc ggcagcgcgt gcccgatgta
      301 gacgccggcg tctatgcgca gatcaccgag agccgaaagg cgcagctcaa tatcgagaac
      361 atcttcaaca agggctattg ggcctcggcg gatggcaaca acaatatctc gccgggtcaa
      421 ccgcggacct tgcgcttcaa ggtcaccgcg aggttgtgag gacgcagcga aagcgttgat
      481 tgacgcctcg ctacttggtc aaaccggaaa tcccgacaat aaggaatgaa gcgagcacgt
      541 agcccgcgat cttgtggaca tagaaatagt agcgcggcca tcccatgaga tcaatcttgt
      601 agtgggcatc gcgcagcttg atgatcggca acagcatatc gaagctatag gagaggccga
      661 cgggcatgcc attcttgggc ccctctccgg agacccccag aacgaacacg ccaagaatga
      721 ccagaacaag agtccatatc atcgcgagct cgatgtgatg accgtagccg atggcccagt
      781 tgagcgtcgt cagccaggcg tacctgaggc cccccgactc gctgacctcg cgctcccggc
      841 cgtcgtagcg gatggccctg gcgtcgtcgg cgtggccctg gttctgaaaa gccagggcaa
      901 gctgctcgta agcgtgtggc gagaaggatt gttgttttcc aaaccagcac cggaacagat
      961 cggatgggca ctccggagtc cggtgatggt ccacggcgat gtcgcgacgt agcgccttgt
     1021 aagtgaatcc attgacatcg acctgggccg gccacgcatc cgtcaaaatc gggattgttt
     1081 cagcccgtgc atttcgcagg atgagcgttt tgcccgggct ccactgcggt gaatgctgcg
     1141 ggactgcttc accgagcgcc agttcgccgc tgagttgtgc gctgctgaga tccacgtcac
     1201 cgccaaatgt gctattgtca aggtagaggc tcctgaattt ggcaaactgg cagtcgatcc
     1261 taccgctgaa ttgggcgtca tacagaaggc cgtcttcgtc gaccactaaa ctgtaacact
     1321 ggagattgcc gcccaccttc gcatggacca ggttgagttg gccgccgacg cgggtactcg
     1381 taaggttgac atcggcgaac ttggcgtcgt tgcgaaggaa cagcgagctg tcgacacgca
     1441 gcttgtccat attgagtgta cgggacacct tcgagccatc taactcgagc gtcccgctaa
     1501 tgcgcgcgct tctgagcaca acctcgccaa attgcccgtg ccgcatgtac acagagctgt
     1561 cgacgcgaag cttctccata tcgagtgtac gggacaccct cgagccatct aactcgagcg
     1621 ccccaatgtg ggcgtctctg agcacaacct cgccgaactg tccgtcccgc atgaataaag
     1681 agctatcgac gttgagcatg ttcatattga gtgtaccggt caccgtcgcg ccatcgaact
     1741 caagcggccc gccaaggcgg tcgtttctga gcacaacgtc gctaaattgt ccgtgccgca
     1801 tgaacacaga gctgtcgacg cgaagcttct ccatattgag tgtacgggac accttcgagc
     1861 catctaactc gagcgcccca atgtgggcgt ctctgagcac aacctcgccg aactgtccgt
     1921 tccgcatgaa caaaggctta tcgacgtgga gcatctccat attcagtgta ccggtcaccg
     1981 tcgagccatc gaactcagcc gccaacgcgg ccgtttctga gcataacctc gcgaaactgt
     2041 ccgtcccgca tgaacaaaaa actattgacg tggagcatct ccatattgag tgtaccggtc
     2101 accgtcgagc catcgaactc aagcgtccgg ccaatgcggg tgcctcccat cgcgacgtcg
     2161 gcgaaccggg cgtcgggcat gaacacggag tcgtcgatcc gtgacccatt catggtgaac
     2221 tcgcccgtca ccttcgatcc gtcaaagctc aacaaattgc tcgactttgc tccgctgaag
     2281 tcgacgcctt gttccagcag gcagcggttg agccaaagct cgtgcttgag ctccgcattc
     2341 accaggtcga tgcgctcctt gaagcgggcg ccgatgatgc gcacgccgcg gtgcggcagc
     2401 gcgctgcggt atttgtcctc gagcaggatg gtctcgagaa acctggagct caagatgcgg
     2461 ctctcgggca ggccttccgg cccgttggga tcgaggtttc cgccgtaggc ggttccctcg
     2521 ttgaagtccg ctatggcccc actgcacacg cgatcccaaa cgaatatctc ttgcttggtc
     2581 cagcgctgag tatcggcggc aacggggcac tttccgtcgg cggacggggc tgccggccgc
     2641 ccttcgccga gggcctcgaa tgaagcgccg agcaacacga gcgagataag aaaaaagcac
     2701 gacgtaccga cccgttcttc ggccacgtca atgagcacac cccacgtccg cccatgtcag
     2761 tctctgcaat gccggccaag gaccatggcc agcgccgcca tagagttgct tcccgaacag
     2821 catagctgcg acttatgggt gtccgtgtga tctagttcac actctcaaat cagaggccgg
     2881 agaacagcat tcgcagtgcg gtgatctttt ttgtcacact taaccggtga tgcatttttt
     2941 ctgcgcgtga gcaccgcctt ggccgttttg agattgatga caagttcgaa tttggcgggg
     3001 cgggaacccc agagccttcc gggcgttcct cccttagcgc aaggcggcaa cgtttttgct
     3061 gtttccgttg cctggtcgct tgccgaagcg gaacagcgtc aggcaagtgg acgccgcgct
     3121 ccaaaccgga gctttgtgga ataccgtgct ctccttgcgc agaatcgccg gagcggcggg
     3181 cgcacagctt cgctcggctg tcctcgcgcg aagctttcta ggccagtctt ttcagctctc
     3241 tttcaactgc gtctgctgag tgtccgacgt tcttcacccg gcgccgtgct ctcacggatt
     3301 cactctgccc tgatgttcgc tgccttgatg atcggccacc acttctcgat ttcggccttt
     3361 tgaaaagcgc ggagcatttc cggcgtctgt tggtcgcgcg gcggcacttc ctgaccgagg
     3421 tcagcaaggt gctggcgcac tgtcggatgg gctagggcat caacgacagc agcattgagc
     3481 ttcgcaacgg cattcttggg tgtgcctttg ggcgcccaca tgccggacca gatagaaatg
     3541 taaaatcttg gaagcccgac ctcatccacg gttggaattt cgggcgccga cgctaagcgg
     3601 gcagcggccg tgacggcata tgctttgatc ttgctggcgc gcacgtgcgg cagggcattc
     3661 aacgcttggt caaacatcag gtcgatctgg ccagcgatca aatcctgcat tgccggagcg
     3721 gcaccgcgat aaggcacgaa ttgaaagcgc gtgccggtct ctttctggaa ataagcgcca
     3781 ctgacgtgcg ctgggctgcc cacgcctgca gttccttgag aggccttgcc tggattcgcc
     3841 ttcaaccacg cgatcaactc attcaagctc tttgcgggcg tagcgttctt tgcgacgatg
     3901 agttgaggat tgcttgcgag caacgcgatc ggctcgaaat cattcagcac gtcgtactga
     3961 agcgggtaga tagcgccgtt taggacatgc gtgcccaaga agccgacgct gagagtataa
     4021 ccatcgggta tcgcacgcac gacgcggccc acgccgatgg tccctccggc gccagttacg
     4081 ttttcaatga tgatgggctg gccgagcgag agcctcatgc gttcggccac aatgcgcccg
     4141 accgtatccg tcggcccgcc agcaggaaac ggcacaacca tcgtgatcgg gcgcgtcggg
     4201 taggcttgcg cctcctgcgc acgcactgcg accggccaag ccgcagctgc gctgccgacg
     4261 aacgtgatga attcgcgtcg tttcatcgga tcacctcgcg ccagaccagg actgtcctga
     4321 atcgaggtgg cgcgctcggc gcctccggcc cgctctaaaa attctcgtcg atgatctttg
     4381 ccagccgaat tctcgcgttc agcagctgtt gctgcaccac cggcagcgcg tgctcaatgt
     4441 agacgccggc caagccgtcg ccgttgcttt gccgcgaatg gacgcgacgt gccaggagcc
     4501 gacacccatg tgggtgattt tattgggata ctttattttc tctccggtct tgggggctgc
     4561 agcttgggta cgatcggagg cctcgcccgc gcctctttct ccgcacggac aacaaatgca
     4621 cgccgcgccg cctccacgcg cttcggcctg cgcgcccgcg catccttctc cgcacgggcg
     4681 gagaacgcgc gcagcccggc cgctgtcggc tccgccttgc gtcctcggcg tgcggcgact
     4741 gcagctagtg gcaagcgcgt atcgagcttg gcgaagcggc ggcgctcttt gatgtcctta
     4801 cgccgtccga cgatgcgata ggagaacgca atgttcgact tgccgcccat cagttcgcgc
     4861 acctcgaagc tggcggcgct cttgcggcgc acatagagtc cgcggcaatc gccctcaggg
     4921 gcggggaata cacgatagtc gctgcgcttg atcgccttgg cgaagtccgc atcgagcctg
     4981 accacggcgc ggccgcgttt gagcttcgcc gtgccgaaat cctcgaacca atgctccgga
     5041 ctctccatgc aatgcagcac gcgttttgaa ccgtcgggaa acggcacgat cgcgtccttg
     5101 accccggcaa atatctggcc agtgaccgag agatcgccaa ggaagtaacc ggcatagcct
     5161 gcgggtgcac gcttggtgga ctggccggcg acgccgatga tgttggagaa gccgaacacg
     5221 cctgcggccg tattcgatgt gccgatcacg ccaggccgcg catccgaagt gcccacgacg
     5281 cccgctatcg tcggctggcc gtcgggaagc ggcggccctt ccgtgccttc cgggccggac
     5341 cctcccgtga ctccggtgtc gtttgtcgac cagcccaaca cgccgggttg ccatgccgag
     5401 gctccctgca cgccatagcc actgtctgac acgcctaaaa cagctgtgcc ctggtacgcc
     5461 cagccctcga cgccgttcca agttgtcgac cacccaacca cgcccgaccc ggtggtcgcc
     5521 gcgccgacga cgccagccga gaggttttgc ggaatcgacg cgtcggcatc ctcttcggtc
     5581 tgtccataga caccgaaatt gttgacggat gtgcccgcga cgccggtgac gaatatcccc
     5641 gtgcctagca cgccagcaaa attcgaatag tcgttaactt ccgggccatc cttgaattcg
     5701 cttttgccaa tgaccggcgg ccaaacctcg tgttgagcgt ccgcgtccac gctcaagccg
     5761 ccagagccgc gaacgccgta gaaccagccg ccgaactggc cgccgatctt cacgtgtggg
     5821 tcattgccaa tgccactggc caagaagccc tggtcgggcg catatgtcgc ctcgcccgga
     5881 aacgcccaaa aaacggcccc ggacggccaa tcgccgccat ggtgactaaa ggcgataaag
     5941 ttattctcat cggtggtcga cattagcacc tcctatcgtg aataaagcga acaatgagca
     6001 gaccatctcg gcaggtccgc tgccgaacag gtcacgcgcc gtcccgcacg gccattacgc
     6061 ggcgcgcccg ctcctaaaaa ttctcgtcga tgatctttgc caagcgaatt cccgcagcaa
     6121 atagtccctc tcctcagcgc cgtcattctc ctgtccgtat tcctgaagca ctgacttgat
     6181 ctggtcgtag agaacggccc attcctgaca ctaccccgca tcgagttcac tggtcatgtg
     6241 cgcattcctc ttactcgctt ccccgcccgc cggttcgaag ccgatagaac acgcgcagcc
     6301 gccttatgac ttcacgatac aaccgtatac gaggatcttc cgattccgca attgccttca
     6361 gcactgcacg cgcatgatcc ggggtcattt gttcgggttc gctgacattt tgctctccca
     6421 tgaaacgctt taagagctct ccggtcgccc ggttgtattc ttggtgaaag ctatcaaatt
     6481 catgacgcct attgttaaag ccgagataca ggtatgcggt ctccaaaacg gcaagattcg
     6541 tacggcttga tctgtttcac ccaagtccgg taggctgaaa ttgcttttgg gacctgcgct
     6601 tgcagcg
//
LOCUS       DATLJT010001431         5184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1502529,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001431 DATLJT010000000
VERSION     DATLJT010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502529"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VM910_32895"
     CDS             <1..176
                     /locus_tag="VM910_32895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI67338.1"
                     /translation="SQLRSDLATHYLSDPNLPISEIAWLVGYQGVSAFSHGYKRWTGM
                     NPKKMRGKLLASH"
     gene            1636..2625
                     /locus_tag="VM910_32900"
     CDS             1636..2625
                     /locus_tag="VM910_32900"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67339.1"
                     /translation="MKRREFIMMLGGAASAWPLGARAQQIGRVRRIGMLNDYGEADSV
                     GQAQVAAFREELHKLGWTEGGNLQIDYRSAAADADLVRTYAAEMVARGPDVVMAAGAT
                     IVAALQRASRSVPIVFVNVTDPVGGGLVASLARPGGNATGFTQFEFGISAKWLELLKE
                     IAPGITRVAVIRDPTARTGGGQLGAIQAVAPSLGVDLSPIDPRDAVEIERGLTAFSRD
                     MKGGLIVTTSRLARAHRDLIISLAARHHLPAIYPFRVYVAAGGLICYGADSTAPYRRA
                     AGYVDRILKGEKAADLPVQAPTKYDLVINLKTAKALGIDVPPTLLARADEVIE"
     gene            2622..3608
                     /locus_tag="VM910_32905"
     CDS             2622..3608
                     /locus_tag="VM910_32905"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67340.1"
                     /translation="MIQRRHFITLLGGAAAAWPRVAHAQQPTIPTVGFLSTASAGSIA
                     PFVEGFRRGLGAAGFVEGRNVAVEYRWADNQHDRLPTLAAELVAQRVAVIATGGATAA
                     ALAAKAATSTIPIVFAIGADPVKLGLVDSMSRPSGNVSGATFLANDLVTKQLQLLQGL
                     LPPGSVIGVLVNPNNPNTAFDTEKTRAAAASLRLQVQLVNVAAERDIDAAFDNLARAR
                     SAALLVLPDTLFIDLRERLAEIAAARKLPAIYSNRLYVADGGLMCYGSSPIEAFREAG
                     IYAGRILRGENPSDLPVVQAVRFELVINLKAAKALGLDLPPTLLALADEVIE"
     gene            complement(3859..4572)
                     /locus_tag="VM910_32910"
     CDS             complement(3859..4572)
                     /locus_tag="VM910_32910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HVI67341.1"
                     /translation="MTWSIIARDGHTGQFGIAVATRFFAVGALVPHIKSRIGAIATQA
                     LVNPFYGTEGLRLLEQGRSARDVVSAITDADMGRDHRQVHVMDAQRGIAAHTGKACVH
                     WCGHLTGDDISVAGNMLLNPGVIEDTARAYERNNALSFPRRLIAALRAGEAAGGDKRG
                     KQSAALVICGDEEWPDLNLRVDDHTDPLGELERLESVSREYFVHVCRFLPNRRDRVGV
                     TDLALIDREAKALAAQDSS"
     gene            5002..>5184
                     /locus_tag="VM910_32915"
     CDS             5002..>5184
                     /locus_tag="VM910_32915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HVI67342.1"
                     /translation="MTTRRAACSCGQLHLTIEGEPSRISMCHCLECQRRTGAAISNQA
                     RFRREQVTFAGKSTAWM"
BASE COUNT          985 a         1596 c         1579 g         1024 t
ORIGIN      
        1 tcagtcaact ccgctccgat ctcgccacgc attacctgag tgaccccaac cttccgatct
       61 ctgagatagc atggctcgtt ggttatcagg gagttagcgc attctcccat ggctacaagc
      121 ggtggaccgg aatgaacccg aagaagatgc gcggcaaatt gctggcgagc cactagcgga
      181 gcttcggcca aagtacgagc cggcgattaa cctgaagact gctaagcgct cggtctcgaa
      241 gtgccgccga tgctccctgc ccgcgcccac gacgtgatcg aataagcggt tccttactgc
      301 ggtgcattaa ggcgggattt gcactaagca gacatgcgga tacgtctgct cgttgtccgc
      361 tttgggaggt aaagcggaca tggcttcgtg atgatgccat gtctgctttg accgtggcgc
      421 taatcgctcc tgccgacgag gtgaccgaat aacgaagtta tttgctgccg tgcgtgtgtc
      481 cgcttttggc acaaagcggc agagggcgat gccctcgcgg caatgtccgt tttcggcgtg
      541 gagcggacat gcctgggcgc gtggtttcgc tggtctgact cgaaaggaac gtcatcaagg
      601 tcagaattcg tcagtgcagc gacctcgctg ccgggcgtaa agcggacatc taatgctcag
      661 atcgagattt tccgcttttg acccaaagcg gacatgagcc tggtcgaaat cccgcagtgc
      721 agcggtctcc gatatcgcaa gaacgcgttc cgcttggttg ggctagacca atgggcgcaa
      781 tcattctgcg caaatggtca ctcggggtaa gctatcggac ctcttggctc tgcgacgtgt
      841 gcaatcgcgc ctcgttaagc tgcgcttgtt gcgactgact tgcagctttg agttttttaa
      901 ttgtcttgtt gcggcgcctg cgctttggca acaacgaaca aatctttggc gcaaagtaac
      961 aaatcctgca cgagcgaaaa cgacttaaga agagaggaac gggtatcggc agtgctacgg
     1021 cccggccggg ccagcatgct cagggatctt cgtcgacgca ctttccgtgc catcttagga
     1081 gagagtcaaa tgccaacgag gaccgagccc cgctctctta gcgagtacga ccgcgcgatg
     1141 ttacgtgcca cgctcgcctt cccgggcaca gcgccggcag tattcacgaa caaaaatgtt
     1201 cgtgggctgc tcggtgccga cgaaacctgc atgacctgtc cacgatgcgg aagtgccgca
     1261 cagggccgca acgatggtgg caagccctgc caagacttca aatgcgacgc atgctgctac
     1321 gtcttcgcga agagtacgtg agcaacgccg aagcctggct gtgcccttcg ccccgaggat
     1381 tgggtgccca aatgcgcaaa aaagtgacac agatcacaga ggatgcggcg ctgcctccag
     1441 tatatcccca acaaccaaga cactcgcttg gaagaagggg atacggtcat gacctggact
     1501 tcagacagca cgggcgtcct gctttacgca gttctcggtg cgtttcttat gctgtcgtcg
     1561 atatacgcgc cggcgtaagg cgttcggtgc gaccatccct ggggcgttcc tgctgcgcgc
     1621 ccgcgaggta atcgaatgaa acggcgcgag ttcattatga tgctcggcgg cgccgcgtcc
     1681 gcgtggccgc ttggcgcgcg cgcgcagcag atcggccgcg tccggcgcat cggcatgctc
     1741 aacgactatg gtgaggccga ctcggtcggg caggcgcaag tcgccgcatt ccgcgaggag
     1801 cttcacaagc tgggctggac cgagggcggc aacctgcaaa tcgattatcg atcggccgca
     1861 gccgatgccg acctggttcg gacgtacgcg gccgaaatgg tcgcgcgtgg gccggatgtg
     1921 gtcatggccg ctggggcaac aatcgtagcg gcattacaac gagcgagtcg tagcgtgccg
     1981 atcgtattcg tgaacgtcac tgatccggtc ggcggcgggc tagtcgcgag tctggcgcgc
     2041 ccgggcggca acgccaccgg tttcacccaa ttcgaattcg gcatcagcgc gaaatggctg
     2101 gagctgctca aagagatcgc tccgggaatc acgcgagtcg cggtcatacg cgatcccacc
     2161 gcgcgcaccg gtggcgggca gctgggcgcc atccaggccg tggcgccgtc gctcggagtc
     2221 gacctgagcc ccattgaccc tcgcgacgcc gttgagatcg agcgcggcct cacggctttc
     2281 tcgcgcgaca tgaaaggcgg actgattgtg acgacgagca gactggcgcg cgctcatcgc
     2341 gacctgatca tctcgttggc agcgcgccat catctgcctg cgatctatcc attccgcgta
     2401 tatgtcgccg ccggtggcct gatctgctac ggggcggatt cgactgcgcc ctaccggcgc
     2461 gcggccggtt acgtcgatcg tatcctcaag ggcgagaaag ccgccgactt gccggtgcag
     2521 gcgcctacca agtacgacct tgtgatcaat ctcaaaaccg ccaaggcgct cgggattgac
     2581 gtgccgccga cgctcctcgc ccgtgccgac gaggtcattg aatgattcaa cgccggcact
     2641 ttatcacgct gctcgggggc gcggcagcgg cgtggccgcg cgtggcgcac gcgcagcagc
     2701 cgactatacc gacggtcggg tttctcagca cggcctcggc cggcagcatc gcgccttttg
     2761 tcgagggctt ccgccgggga cttggtgcag cgggcttcgt tgagggcagg aatgtcgccg
     2821 tcgaatatcg ctgggcggac aatcagcacg atcgcttgcc gacgctcgcg gccgaactgg
     2881 ttgcccagcg ggtcgctgtc atcgccaccg gtggcgccac agccgcggcg cttgccgcca
     2941 aggccgcgac ctcgaccatt ccgatcgtgt tcgcgatcgg cgccgatcca gtgaagctcg
     3001 gccttgtcga tagcatgagc cggccaagtg gcaacgtcag tggcgcgact ttcctcgcca
     3061 acgatttagt gacgaaacaa ctgcagctgc tgcaggggtt gctgccgcct gggagcgtga
     3121 tcggcgttct ggtcaatccg aacaatccca atacggcgtt cgataccgag aagacgcgag
     3181 cggcggccgc gtcgcttcgg ttgcaggtcc aacttgtcaa tgtcgccgcc gagcgcgata
     3241 tcgacgccgc gttcgacaat cttgcccgcg cgcgatcggc cgcgctcctc gtcctcccag
     3301 acacgctgtt catcgatctg cgcgaacggt tggccgagat cgcagctgct cgcaagctgc
     3361 ctgcgatcta cagcaaccgc ttgtacgtcg cggatggcgg gctgatgtgc tacggcagta
     3421 gcccgatcga ggcgtttcgc gaagccggca tctacgctgg ccgcattctg cgaggcgaga
     3481 acccctccga cctgccggtg gtgcaggcgg tcagattcga gctggtgatc aatctcaagg
     3541 ccgccaaggc gctcgggctc gacctgccgc cgacgttgct cgcgctcgcc gacgaggtga
     3601 tcgagtaatc actgccgcca tgcacggcgt ttgctgcagt gcatgagtcc gcttttggca
     3661 ctaagcggac atgctcatcg tgtcggtcga tgtccgcttt cgggggcaaa gcggacatgg
     3721 cctcgtgacg gcgtgaggtc tgcttatgac ccaaagcgga catcgagcgg cacattaacc
     3781 gtatgtgttt tgggtccgcg atcatcgtga aagacgagtt tgactttccg atttcaagga
     3841 ccggaccgag cgcgcccctc acgacgagtc ttgcgcggca agggctttgg cttccctgtc
     3901 gatcagggcg agatcagtca ctccgacccg gtcgcggcgg ttcggaagaa atcgacacac
     3961 atgcacaaag tactcacggc tgacgctttc aaggcgttct agttcgccca gtggatccgt
     4021 atggtcgtcg acgcgcagat tgagatccgg ccattcctcg tcaccgcaaa tcaccagcgc
     4081 ggccgattgc ttcccgcgct tgtcgccgcc ggctgcctcg cccgcacgca acgcggcaat
     4141 gagccgtcga ggaaacgaga gtgcgttatt tctttcatag gcccgagccg tgtcttcaat
     4201 gaccccgggg ttgagcagca tattgcccgc gaccgagatg tcgtcacctg tgagatgccc
     4261 acaccagtgc acgcaggctt ttcctgtatg cgcggcgatg ccgcgctgtg cgtccatcac
     4321 atggacctgt cggtgatcgc gtcccatgtc ggcgtcggtt atcgcggata caacgtcccg
     4381 cgcggacctc ccctgttcca gcaatcgcaa gccctcagtg ccgtaaaatg gattgaccag
     4441 tgcctgtgtc gcgatcgcac cgatgcgaga tttgatgtgt ggaacgagcg cacccacagc
     4501 gaagaaacga gtcgcaacgg caattccgaa ttgtccagtg tgcccatcgc gagctatgat
     4561 tgaccaggtc atgccagcca tccgcagttg gggcgatcga actgtccgtt ctctcatcgc
     4621 caccttatta gactaacaga taaaagctca cgccgtgacg gctacgggtg gccctcaact
     4681 gccgatctgc cagtaatgcg cccaaccaag ttcgagtctc gcgatcaatc tcgaggccgc
     4741 gaaggagctt ggcctgacac tgccgcaaac tctgcaagtc gcggccgacg aggtgattga
     4801 ctaagagcat ctttagctgc aatgcgaatg tccgcttttg ggcacaaagc ggacctgcta
     4861 gtcgtgtcgc tcgaggtccg ctttcggggc gtgccagaag ccgacactag ctggtcatag
     4921 gattccgctg cccctgcccc tggtgcatcg tttcgaagtc cagcctacac tccgccatat
     4981 cgacagccac ggagccagtc catgaccacc cgacgcgccg cctgcagttg cgggcagctt
     5041 catcttacga ttgagggcga accttcgcgc atctccatgt gccactgcct ggagtgccaa
     5101 cgccgcactg gcgccgcgat cagcaaccag gcgcgcttcc gccgcgagca ggtcaccttc
     5161 gccggtaagt ctacggcgtg gatg
//
LOCUS       DATLJT010001432         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2653819,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001432 DATLJT010000000
VERSION     DATLJT010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653819"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VM910_32920"
     CDS             complement(<1..1052)
                     /locus_tag="VM910_32920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI67343.1"
                     /translation="MSALPLSGIRILDLTRVLAGPLSAQMLADLGADVIKIERPGGGD
                     DARAFGPPYLEDPEGKENNNNSFYLCANRNKRSVTVNIARAEGQEIIRELAKSCDVFM
                     ENYKVGDLKRYGLDYDSIRAVNPGIIYCSVTGFGQTGPYAPRAGYDAILQAMGGLMSV
                     TGHIDGEPGEGPMKVGPSIVDYMTGMNTSIAILSALYHRDANGGEGQRLDVCLFDTVI
                     ASLSHWLQIYLVSGKIPPRRGTWGNGGMPAGVFRCTDGELMVVVGNDAQFARTCQVLG
                     APELATDPKFLKNNDRVKHGKEIMAIYAGLFLKKPVAYWLDELEKAGVPSGPINDFAQ
                     VFADPHVRSRGMQIKV"
     gene            complement(1368..1514)
                     /locus_tag="VM910_32925"
     CDS             complement(1368..1514)
                     /locus_tag="VM910_32925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67344.1"
                     /translation="MTSKKAAAVLWTPVSHAIHIDGAELPVLQDVHLSVEPGEYWHQR
                     TTSK"
     gene            complement(1511..1903)
                     /locus_tag="VM910_32930"
     CDS             complement(1511..1903)
                     /locus_tag="VM910_32930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI67345.1"
                     /translation="MKREVIRVEPITSYLERFKAPASAVTRAGNMVFVSGLPPFDPDT
                     GEIVNAGIERQTEIVMEQMRRCLKAAGASLDNVMKCNIFCTSAKHFAAVNAIYARYFP
                     VDPRARIYVCIPEWTGSFDIEIDCIAIV"
     gene            complement(1988..2359)
                     /locus_tag="VM910_32935"
     CDS             complement(1988..2359)
                     /locus_tag="VM910_32935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HVI67346.1"
                     /translation="MHKIPEPGAPAAPQITFDQFLAVDIRVGTIVAVEAFPEARKPAF
                     KLTIDFGPILGAKRSSVQITQLYEAASLVGRQVAAVVNFPPRQIGKFMSEVLTLGFPD
                     EQGNVVLFAPDEKVPNGGRLF"
     gene            complement(2384..>4042)
                     /locus_tag="VM910_32940"
     CDS             complement(2384..>4042)
                     /locus_tag="VM910_32940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVI67347.1"
                     /translation="GEVLIPLDQDSLGEAIGAIKTSGATSVAICFLHSYRNPAHEIAA
                     RERLMRELPDVSVSCSSDVLPQIKEYERVSTTVVNAYVEPVVRHYLERLERRLIEAGL
                     KGALFIVLSHGGMAPVEEAARLAAATVLSGPAGGMSGCRRCAAMLGIPDLVAFDMGGT
                     STDISLVTGGRVTLSADGGLAGERIALRSLDIASLAAGGGSIASVDAGGIFRVGPESA
                     GAVPGPAAYGQGGTAATVTDANVVLGYLDAKAFMGGKRPLDVRAAEAAIDRVAATLNI
                     TRIEAAAGIFRLVNLKMADGIRLMTVRRGVDPRRFALLSFGGAAGLHAVELARELELT
                     RIIVPTTASVLSAWGMLTSDLRYEQSRTHFESGARTTAGEIRDMFAELETRATNRLRS
                     WFDGRIAVERSAEMRYGEQIFEIDVPLDGIDLNSPTLIADIEQRFHIRHEELYTYASR
                     DQEVVFVNARVAAIGGVAVGEPEAGAVPSSAPGVPRSRRQAFFGAWREVDVFAFSDLK
                     PGDRLSGPAIIEAETTTVLINEGDQLSVNALGWLDIRVSRARPA"
BASE COUNT          758 a         1315 c         1264 g          705 t
ORIGIN      
        1 tcgaccttga tctgcatgcc gcgcgagcgg acgtgcggat cggcgaagac ctgcgcgaaa
       61 tcgttgatgg gaccagaggg cacgccggcc ttctccagct cgtccagcca ataggcgacc
      121 ggcttcttca gaaacaggcc ggcgtagatc gccatgatct ccttgccgtg cttcacgcgg
      181 tcattgttct tgagaaattt tggatcggtc gcaagctccg gcgcgccgag cacctggcag
      241 gtgcgcgcga actgcgcgtc gttgccgacc accaccatca attcgccatc ggtacagcgg
      301 aatacgccgg ccggcatgcc gccattgccc caagtgccgc ggcgcggagg gatcttgccg
      361 ctgacgagat agatctggag ccaatgcgac agcgaggcga tcacggtgtc gaacaggcag
      421 acgtcgagac gctgcccctc gccgccattg gcgtcccggt gatagagcgc agagaggatc
      481 gcgatcgagg tgttcatgcc ggtcatgtag tcgacgatgg aggggccgac cttcatcggg
      541 ccttcgccgg gctcaccgtc gatgtgaccg gtgacgctca tcaacccgcc catcgcttgc
      601 aggattgcgt cgtagccggc gcgcggcgcg taaggcccgg tttggccgaa cccggtcacc
      661 gaacaataaa ttatgccggg attgacggcg cggatggagt cgtagtcgag cccgtagcgc
      721 ttgaggtcgc cgaccttgta gttttccatg aagacatcgc aggatttcgc gagctcgcga
      781 atgatctcct gcccttcggc gcgcgcgata ttgacggtca ccgatctctt gttgcggttg
      841 gcacagagat agaaggagtt gttgttgttc tctttccctt cgggatcttc gaggtacggc
      901 gggccgaagg cgcgcgcgtc gtcgccaccg ccgggccgct cgatcttgat gacatcggcc
      961 ccgagatcgg ccagcatctg cgcggacaag ggtcccgcca gcacccgcgt cagatcgaga
     1021 atcctgatgc ctgaaagcgg cagcgccgac atgaatcttc ctccgaaaaa ttaggggctt
     1081 ttggctcgca cgggatcaag cgtgactgtc gccggtgcga accccctgcg gatacaccat
     1141 ttttagcgcg caagcactgc atttcgggca tgcagccatc catcccgcag ccggcgcagc
     1201 ggcccgctgc agttccgcgc ctttggtggt gggggcggag accgagcgca tcgagccttc
     1261 cgccgcgacc gcacggcgcg cagtggagcg gatttgacgt tcgctaccga gccaaccgcg
     1321 agttcgtcag gcgaaccacg tcaaatgcgg aaccccactg gcatcaatca tttgctggtg
     1381 gttctttgat gccaatattc gccgggctcg accgagaggt gcacatcctg caacaccggc
     1441 agctcggcgc cgtcgatatg gatggcatgg ctaacaggcg tccagaggac ggccgccgcc
     1501 tttttcgaag tcacacgatc gcaatgcaat cgatctcgat gtcaaacgat ccggtccact
     1561 cggggatgca gacatagatc cgcgcccgcg gatcgaccgg aaaatagcgc gcatagatcg
     1621 cgttcaccgc ggcaaaatgt tttgccgagg tacagaagat gttgcacttc atcacgttgt
     1681 cgagggaagc gccggcggcc ttcaggcaac gcctcatctg ctccatgacg atctcggtct
     1741 gccgctcgat ccccgcgttg acgatctcgc cggtgtcggg atcgaacggc ggcaggcccg
     1801 agacgaacac catgttgccg gcgcgggtca cggcagaggc cggcgccttg aagcgttcga
     1861 gataggaggt gatcggctcg actcggatga cttcgcgttt catggggcac ctgctcgact
     1921 gtgaggactt aagcctagcc cagaagcgtt acatcatatt tcggaatcga tggaagcgtc
     1981 gcggcgatca gaacagccgt ccgccgttcg gcaccttctc atccggcgcg aacagcacga
     2041 cattgccctg ctcatcagga aagcccaagg tcaagacctc cgacatgaat ttcccgattt
     2101 gccgcggcgg gaagttcacg acggcggcaa cctgccgccc aaccaggctc gccgcttcat
     2161 aaagctgcgt gatctgcacg ctcgatcgtt tggcgccaag gatggggccg aaatcgatgg
     2221 tcagcttgaa cgccggcttg cgtgcctccg gaaatgcctc gaccgcgacg atcgtcccca
     2281 cccggatatc gacggcgagg aactgatcga aggtgatttg cggcgcggcc ggcgcgccgg
     2341 gctccggaat cttgtgcatg aaaaatactc catgaagttg cgcttatgcc ggccgcgccc
     2401 tggacaccct gatatcgagc cagcccagcg cgttgaccga gagttgatcg ccctcgttga
     2461 tcaggaccgt cgtggtctcg gcttcgatga tggcggggcc tgaaagccga tcgcccggct
     2521 tgagatcgct gaaagcaaag acgtcgacct cgcgccaggc gccgaagaag gcctgacgac
     2581 gggagcgtgg cacgccaggt gcggacgatg ggaccgcccc cgcctccggc tcgccgaccg
     2641 cgaccccgcc gattgcagcg acgcgcgcgt tgacaaacac cacctcctga tcgcgcgagg
     2701 cgtaggtgta gagctcctcg tgcctgatat ggaagcgctg ctcgatgtct gcgatcagcg
     2761 tcggcgaatt gaggtcgatc ccgtcgaggg gaacgtcgat ctcgaagatc tgctcgccgt
     2821 agcgcatctc ggccgagcgc tcgaccgcga tcctgccatc aaaccagctc cgcaagcggt
     2881 tcgtcgcccg cgtttcgagc tcggcaaaca tgtccctgat ctcgccggcc gtcgtgcgcg
     2941 cgccgctctc gaaatgcgtg cggctctgct catagcgcag gtcggaggtc aacatgcccc
     3001 acgccgacag cacggaggcc gtggtcggca cgatgatgcg cgtcagctca agctcgcgcg
     3061 cgagctcaac cgcatgaagc cccgcggctc cgccaaaact cagaagcgcg aagcggcgcg
     3121 gatcgacgcc gcgccgcacc gtcatcaggc ggatgccgtc ggccatcttc agattgacca
     3181 ggcggaaaat gccggcggcc gcttcgatcc gcgtgatgtt cagcgtagcg gcgacgcggt
     3241 cgatcgccgc ctctgcagcc ctgacatcga gcggtcgctt gccgcccata aaggccttcg
     3301 catcgagata gccgagcacg acattggcat cggtcacggt tgcagcggtc ccgccctgac
     3361 cgtaggccgc cggtcccggt acggcaccgg cgctttccgg accgacgcga aagatgcccc
     3421 ccgcatccac ggaggcgatc gagccgccgc cggcagccag gcttgcgata tcgaggctgc
     3481 gcagcgctat ccgttcaccc gcgaggcccc cgtcggccga cagcgtcacc cggccgcccg
     3541 tgaccagcga gatatcggtc gaagtgccgc ccatgtcgaa ggccacgaga tcggggatgc
     3601 ctagcatggc ggcgcaacgc cggcagcccg acatgccgcc cgcgggtccc gacagcacgg
     3661 ttgcggcggc aagccttgcc gcttcctcga ccggcgccat cccgccatgc gagaggacga
     3721 taaagagcgc tcccttcagg ccggcctcga tcaggcgccg ctcgagccgc tcgagataat
     3781 gccggacgac aggttcgaca taggcgttca cgaccgtagt cgacacgcgc tcatattcct
     3841 tgatctgcgg aagcacgtcg ctcgaacagg acacgctgac atcaggcagc tcgcgcatga
     3901 gccgctcgcg cgctgcgatc tcatgcgcgg gattgcggta ggaatgcagg aagcagatcg
     3961 cgaccgacgt tgcgcctgat gtcttgatcg cgccgatcgc ctcgcccagc gaatcctgat
     4021 cgagcggaat caacacctcg cc
//
LOCUS       DATLJT010001433         2934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2192969,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001433 DATLJT010000000
VERSION     DATLJT010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192969"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..919
                     /locus_tag="VM910_32945"
     CDS             41..919
                     /locus_tag="VM910_32945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HVI67348.1"
                     /translation="MSDDGLLSSLRYLFADFLDEEDQTPPFLPDGLPEGAAPIVSDGI
                     HLLMDYQGASYARLYVERLTRFVGKQGIGAAIFGEIARLMANRMAYEDAIRIAQLKLA
                     QYHDSGGAVRSTDIEKFRFDELVDALPAVAAEPVLMVLDKLGWEHRRVSIPFSTASKW
                     GIRRLKVEAWLRRWRMFSIRYLQERAWVERWLHMIDRCLAKQPQSVPAIVETATMVQG
                     FGDPYRQGLAAWHAIIDGLAKPTFDGVLPLTDLAGAIEEARAAAMPDPRQVALKRTIA
                     QIRARAQGAGANAAAG"
     gene            complement(959..2368)
                     /locus_tag="VM910_32950"
     CDS             complement(959..2368)
                     /locus_tag="VM910_32950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI67349.1"
                     /translation="MSGDIRKPPEQPRPRSQVARTNRRQVLLYVLLTLLLSLATVGAG
                     RVLLKDSETLTFAVGNPNGEDARFAAKLATVLKNTNSRFRLKIVTSADNAKALAQFDR
                     RAADLAVLRTDAKIPARARALAILDHDLVLLLSPGSKKIKSLDDLKKKKIAVWADSED
                     SLSFVRNIFGLTESPDTAPKVQLAPAGSTLDKLFASGFGVVIAVTHASKVMKDKSYEQ
                     YAKKGGFTLNAIESAKALARKIPGITEETIETGILSSSPEIPDDDLDTIGLEWLLVAQ
                     SRISTTTAANLARIIYENKSELVLDDGFASKIEPAATDKDAFIIAHPGAAEYINDNIK
                     SFMDRYSDMMYLGAGALSVIGSIFAAIYAKVTRIAPERASELATAILIVGEKIEHADT
                     LEALEELQNELERILRGAVIGLRDGTISGDGLDTFKLGYELVRNEIELRRDYLTRHPG
                     DKTPGAEDNVSIVKTARSA"
     gene            2717..>2934
                     /locus_tag="VM910_32955"
     CDS             2717..>2934
                     /locus_tag="VM910_32955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HVI67350.1"
                     /translation="MSFPAMSSSVPVGALVGWMVLLTVKHIIADFVLQTAWMAHGKDQ
                     KTGWALPLLVHCLIHLAVAMVLILIVAP"
BASE COUNT          529 a          892 c          898 g          615 t
ORIGIN      
        1 cgcttgcggc ggcgaagcca gcttgaggga aagcattcgc gtgtctgacg acggcctgtt
       61 gtcatcgctg cggtatctgt tcgccgattt cctcgacgag gaagaccaga cgccgccgtt
      121 cctgcctgac ggcctgccgg aaggtgctgc gcccatcgtc agcgacggca tccacctctt
      181 gatggattac cagggcgcaa gctacgcccg gctctacgtc gagcggctga cgcgcttcgt
      241 cggcaagcag ggtatcggtg ccgcgatttt cggcgagatc gcccgcctga tggccaaccg
      301 catggcctat gaggatgcga tccggatcgc ccagctcaag ctcgcccaat accacgacag
      361 cggcggtgcg gtccgatcca ccgacatcga aaagtttcgt ttcgacgagc tggtggacgc
      421 gcttccggca gtcgccgccg aacccgtgct catggtgctg gacaagctcg gatgggagca
      481 tcggcgcgtc tcaataccgt tctcgacggc cagcaaatgg ggtattcgcc gtctcaaggt
      541 ggaagcctgg ctgaggcgct ggcgaatgtt ctcgatccgc taccttcagg agcgcgcctg
      601 ggtcgagcgc tggttgcaca tgatcgaccg ctgcctcgcc aagcaaccac agtccgtgcc
      661 ggcgatcgtc gagactgcga ccatggtgca gggctttggc gatccctatc gccagggatt
      721 ggccgcctgg cacgctatca ttgacggact ggcgaagccg accttcgacg gcgtactgcc
      781 gctgaccgat ctcgccggcg cgattgagga agctcgcgcc gccgcaatgc ccgatccccg
      841 acaggttgcg ctcaagcgca ccatcgcgca aatcagggcc cgcgcgcagg gcgcgggcgc
      901 caatgcggcg gccggttaga gggacgattc tccctctccc gaaagagcac gccgaacctc
      961 aggcgcttcg cgccgtcttc acgatcgaga cgttgtcttc cgcacccggc gttttgtcgc
     1021 ccggatggcg cgtgagatag tcgcggcgca gctcgatctc attgcgcacc aattcgtaac
     1081 cgagcttgaa agtatcgagg ccgtcgccgg aaatcgttcc gtctctcagt ccgatcaccg
     1141 cgccgcgcag gattcgctcc aattcgttct gcagttcttc cagtgcctcg agcgtatcgg
     1201 catgctcgat cttctcaccg acgatgagga tcgcggttgc gagctcgctc gccctttcgg
     1261 gtgcaatccg cgtgaccttc gcgtagatcg cggcaaagat cgacccgatg acggaaagcg
     1321 caccggcgcc gagatacatc atgtcgctgt agcgatccat gaacgacttg atgttgtcgt
     1381 tgatatattc ggccgcgccc ggatgcgcga tgatgaaggc atccttgtcg gtcgccgccg
     1441 gctcgatctt ggaggcaaag ccgtcgtcga gcacaagctc cgacttgttc tcatagatga
     1501 tgcgggcaag gttcgcagcc gtcgtggtcg aaattctgga ttgagcgact aaaagccatt
     1561 cgagtccgat ggtgtcgaga tcgtcgtcgg gaatttccgg cgaggacgac agtatgccgg
     1621 tctcgatcgt ctcctccgta atccccggaa tcttgcgcgc cagcgccttt gccgattcga
     1681 ttgcgttcag ggtgaagccg ccctttttgg catattgctc gtagctcttg tccttcatca
     1741 ccttcgaagc gtgggtgacc gcgatcacca cgccgaagcc cgaggcaaat agtttatcca
     1801 gggtggaacc ggcaggcgca agctgcacct tcggggcggt gtcagggctc tcggtaagac
     1861 cgaagatgtt gcggacgaag gagaggctgt cctcgctgtc ggcccacacc gcgatcttct
     1921 ttttcttcag gtcgtcgagc gacttgatct tcttggagcc cgggctgagc aacagcacga
     1981 gatcgtggtc gaggattgcc agcgcccgcg cgcgtgccgg gatttttgca tcggtccgca
     2041 gcaccgcaag gtccgctgca cggcggtcaa actgcgcgag tgctttcgca ttgtcggcgc
     2101 tggttacaat cttgagacga aatctcgaat tggtgttttt cagaacggtg gccagcttgg
     2161 cggcaaaacg cgcgtcttcg ccgtttgggt tgccgaccgc gaaggtgagt gtctccgaat
     2221 ccttcagcag aacgcggccg gctccgacgg tggcaagcga cagaagcagc gtgagcagca
     2281 catacagcag cacctgcctg cgattggtcc ttgctacctg cgagcgcggg cgcggctgtt
     2341 cgggcggctt tcggatgtca ccgctcatgg ctctaccccg gaggcctgcc gtttcgccac
     2401 cttcaggtgg cgcccggtaa aatcgtaaac agtcgaaaaa gaatgataat tctgagcctt
     2461 gcgatgatat ccccaaccat gccgattgca aaccggcaac ggcggtaacc ttaccggcga
     2521 ccggcctccg gcatggcgcg ggtaggtcac actttgcgtt tacttcttat ttttcgggtt
     2581 cccggcaggt gcaatccggc cgcggaggtg tcataaaaga tcaacacggg gcgaattggc
     2641 cggtcaaaga agaaaagttg cgctgaacgc ctccaccagt ccccgtgcga tcctaggttt
     2701 ccagagggcg tcagttttgt ccttccctgc tatgtcgtcg tcggttccgg ttggtgcgct
     2761 ggtcggatgg atggtgctgc tcacggtcaa gcacatcatt gccgatttcg ttctgcagac
     2821 cgcctggatg gcgcacggca aggatcagaa gaccggctgg gcgctgccgc ttctggtcca
     2881 ctgcctgata cacttggcgg ttgcaatggt cctgatcctc attgtcgcgc caaa
//
LOCUS       DATLJT010001434         1592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2423546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001434 DATLJT010000000
VERSION     DATLJT010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VM910_32960"
     CDS             complement(<1..762)
                     /locus_tag="VM910_32960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67351.1"
                     /translation="MSDDLILSVDHLTMRFGGIVAVNDLSFAAERRKVTALIGPNGAG
                     KTTVFNCITGFYRPTGGTIRLKRDEANEIALERLNDFRISKTAKVARTFQNIRLFPGM
                     TALENLLVAQHNALMRASGLTFLGLIGAPSWRSAEKRAIDRAQYWLERIGLLARADDA
                     AGNLPYGDQRRLEIVRAMCSEPVLLCLDEPAAGLNARESAALSELLLSIRADHGTSIL
                     LIEHDMSVVMEISDHVVVMDYGVKIAEGSPADVRDD"
     gene            complement(759..>1592)
                     /gene="livM"
                     /locus_tag="VM910_32965"
     CDS             complement(759..>1592)
                     /gene="livM"
                     /locus_tag="VM910_32965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HVI67352.1"
                     /translation="AYSYALIATTFGLSFFECLPLAGFLAALWGVMLGFPVLRLRGDY
                     LAIVTLAFGEIIRLVIINWQSLTGGPNGVSGIPRPTLLGIPLTPGDDGLAARLGIPFS
                     PTHRIVFLFYLILALALITNWVTIRLRRLPIGRAWEALREDEVACRALGINTTTTKLT
                     AFATGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAALTM
                     IGGFELFRGLEQYRMLVFGVAMVLLMIWRPRGLIGHRAPTVYLTKAQAISSDLVKEGR
                     G"
BASE COUNT          312 a          500 c          538 g          242 t
ORIGIN      
        1 atcgtcacgc acgtcagccg gcgagccctc ggcgatcttc accccgtaat ccatcacgac
       61 gacatggtcc gagatttcca tcaccacgct catgtcgtgc tcgatcagga ggatcgaggt
      121 gccgtgatcg gcgcggatcg aaagcagcaa ttcgctcaac gccgcgctct cgcgcgcatt
      181 cagcccggcc gccggttcgt cgaggcagag cagcaccggc tcgctgcaca tcgcgcgcac
      241 gatttcgagc cgccgctgat cgccataggg caggttgccg gcggcgtcgt cggcgcgcgc
      301 gagcaacccg atgcgctcga gccagtattg cgcacgatcg atcgcacgct tctcggcgct
      361 ccgccaggaa ggcgcgccga tcaggccgag gaaggtcaac ccggaggcgc gcatcagtgc
      421 attgtgctgg gccaccaaca ggttttccag cgcggtcata ccgggaaaca gacggatgtt
      481 ctggaaggtg cgcgcgacct ttgccgtctt ggagatgcgg aaatcgttga gccgctccag
      541 cgcgatctcg tttgcttcgt cgcgcttgag ccggatcgtg ccgccggtcg gcctgtaaaa
      601 tccggtgatg cagttgaaga cggtggtctt gccggcgccg ttcggaccga tcagcgcggt
      661 caccttgcgc cgctcggcgg caaaggacag atcgttgacg gcgacgatac cgccgaagcg
      721 catggtgaga tgatcgacgg agagaataag gtcgtcgctc atccgcgtcc ctccttgacc
      781 agatcggagg agatcgcctg cgccttcgtc agatacaccg tcggcgcgcg atggccgatc
      841 agcccgcgcg gccgccagat catcaggagc accatggcga cgccgaacac cagcatgcgg
      901 tattgctcga gcccccggaa caactcgaag ccgccgatca tggtcagcgc ggcgagcgcg
      961 acgccgagtt gcgaacccat gccgccgagc acgacgatgg cgagcaccag ggccgattcc
     1021 tggaaggtga aggattccgg actgatgaaa ccttgccgcg tggcgaagaa ggcgccggca
     1081 aagccgccga acatcgcgcc ggtcgcgaac gccgtgagct ttgtcgtcgt tgtattgatg
     1141 ccgagtgcgc ggcaggcgac ctcgtcctcg cgcaaggcct cccaggcgcg gccgatcggc
     1201 agccgccgca ggcggatggt cacccagttg gtgatcagcg ccagcgccag gatcaggtag
     1261 aacaggaaga cgatgcgatg ggtcggcgag aaggggatgc cgagccgcgc agccagtccg
     1321 tcgtcgccag gcgttaaggg aatgccgagc agggtcgggc gcgggatgcc cgagacgccg
     1381 ttcggcccgc cggtcaggct ctgccagttg atgatgacga ggcggatgat ttcgccgaag
     1441 gcgagcgtga cgatggcgag atagtcgccg cgcagacgca ggaccggaaa gcccagcatc
     1501 acgccccaca gtgcggcaag aaatccggcg agcggcagac actcgaagaa cgagaggccg
     1561 aaagtggtcg caatcagcgc gtaggaatag gc
//
LOCUS       DATLJT010001435         5780 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2655660,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001435 DATLJT010000000
VERSION     DATLJT010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655660"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VM910_32970"
     CDS             complement(<1..192)
                     /locus_tag="VM910_32970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67353.1"
                     /translation="MLAQDQREQARKLFAYLDTQTTAIADTVYRNEVADYTCRKQFAR
                     ERELFFRRGPLFVGLSCLLP"
     gene            complement(241..483)
                     /locus_tag="VM910_32975"
     CDS             complement(241..483)
                     /locus_tag="VM910_32975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67354.1"
                     /translation="MRGFRHTMQISPRNPKGHYIPKRGLVQRVQVPAKFEKVINPKPQ
                     GRASLMTEGAQFVVPDGRRQRNGFSATFRSGYDADM"
     gene            535..2118
                     /locus_tag="VM910_32980"
     CDS             535..2118
                     /locus_tag="VM910_32980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI67355.1"
                     /translation="MSKASLLLVTVLTGLSLAQPVFAQSEDDKLGKVHFETSCNAEAQ
                     KLFDRGMLYQHSFWYRASQKMFEDTLKADPACSIAYWGTALSLLWNPHTAPPAKNLAE
                     GAAAIEKARTIGFKTDREKGYVDALAAMYTDYDKVDHHARVVAYAKAMEALAQRYPDD
                     DEAQIHYALALNTSASPADKTYANQLKGAAILEPIALRQPLHPGVAHYLIHLYDYPSI
                     ADQGIEAARRYAKIAPDAPHALHMPSHIFTRVGYWSESIASNVASARSAKGSSDFSDE
                     LHAMDYLVYAYLQLGQDDKAKAVVDEMGKVTGFSETFIPGPYAEAASPARYVLERGDW
                     KAAEALTVRPSPLANVQAISYFARALGAARAGDPEAAKPDIARLGELRDKLREAKDAY
                     WSEQVDIQARIASAWVLYAEGKKDDALKAMSDAADAEDRTEKHPVTPGTPKPARELYG
                     EMLLDNGRAAEALTAFEATLKKEPNRLGAYAGAARAAAQIGDTAKAREYFGKIVEQAG
                     SGDSTRTEVADARNYLAGR"
     gene            2090..2524
                     /locus_tag="VM910_32985"
     CDS             2090..2524
                     /locus_tag="VM910_32985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67356.1"
                     /translation="MRAIISPGAKSAPPKAHNATRATLSLALSLLAAPAGADSLQIFG
                     YSGYLGEWELTATLKEDGSTTPKNYSGPLSLKHVGICTQDGPEEKSGEIRVQMLSSSS
                     RLQATLSVDGVDCGYQGVLSDFYNGIMSCPGREAVPLKLWIK"
     gene            complement(2629..3156)
                     /gene="nuoB"
                     /locus_tag="VM910_32990"
     CDS             complement(2629..3156)
                     /gene="nuoB"
                     /locus_tag="VM910_32990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017968805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HVI67357.1"
                     /translation="MRRTLFESLIRGPLTEQPPAADDAAIAELAKNVEKAAQARLGRS
                     LSIRQVDAGSCNACELEIHALANAFYDLERFGLRFVASPRHADVLMVTGPVTKNMREA
                     LKRTYDATPDPKWVVAVGGCAADGGIFKGSYACVGGVSDVVPVDLHIPGCPPSPTALL
                     KGLLALLEHVTGERR"
     gene            complement(3164..4669)
                     /locus_tag="VM910_32995"
     CDS             complement(3164..4669)
                     /locus_tag="VM910_32995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HVI67358.1"
                     /translation="MSLLDLIPYQEAIATHRPWPRVLVGEQGWLKAIDLLAAGDVTLL
                     GFWGEVSAVHLVLLGATDEIATVSLECESGKFPSVAARHAPALRLERTIHDLFGLDAV
                     GATDTRPWLDHGVWGRSTPFGKPAAPRARGPYQFLSSEGEGLHQIAVGPVHAGIIEPG
                     HFRFAANGEHVVRLEERLGFVHKGIESLMADTSLDAAAKLAGRTSGDSTVAYSFAFAR
                     AVEAALQINVPPRAIYLRALMAELERLANHFGDIGAICNDASFALMHAQTGILRERVL
                     RAADTCFGHRLMMDVVVPGGVARDIAPEGPAAIRELSAEIRKNFPRLVELYDNTASLQ
                     DRTVATGIVKREYAQKFGAGGYVGRAAGRAFDARRNLAYAPYDELSFEVPVLEAGDVN
                     ARVWIRIREVEQSLALIDQILDRLPPGAFNVALKPGGAPCEGIALTEAFRGDVLVCLR
                     LHSEGRIDRCHLRDASWFQWPLLEAAIEDNIIADFPLCNKSFNCSYSGADL"
     gene            complement(4676..>5780)
                     /locus_tag="VM910_33000"
     CDS             complement(4676..>5780)
                     /locus_tag="VM910_33000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HVI67359.1"
                     /translation="AMNLALVANNIGLMWVAVEVATLTTVLMVGIYRTHEALEAAWKY
                     FILGSVGIALALFGTILVYMAARPVIGEGTDAMVWTVLVARATQFDPALLNVAFVFLL
                     LGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYALLRFKMIMAACEGAIA
                     PGPLMVVMGLISVIFAALMLYRRRDIKRMFAYSSIEHMGIIVFAFGMGGALANFAGLL
                     HMTLHSLTKSAIFFAVGHIAQVKGTQKIADIGGLTVTNPVLGWGLVLGVVAIVGLPPL
                     GIFMSEFLIVSSTFARQPWLTAILVLGILIALGGLFMRLNVVAFGEPKGLTKPAEASY
                     VPMFTHLALVLMAGIYLPPALVAGFSNVARLLG"
BASE COUNT         1198 a         1797 c         1800 g          985 t
ORIGIN      
        1 cggcaagagg cagctcaggc cgacgaacag cgggccgcgc ctgaagaaaa gctcgcgctc
       61 gcgggcgaac tgcttacggc aggtgtagtc ggcgacctcg ttgcggtaga ccgtgtccgc
      121 aatggcagtg gtttgcgtgt cgagataggc gaagagcttc cgcgcctgct cgcgctgatc
      181 ctgggccagc actcgttctc ctcccgccgg cgtcggctcg gtcgcgccgg ccgcttcatt
      241 ctacatatct gcatcatagc cggatcgaaa cgtagctgag aagccatttc gttggcgacg
      301 cccatccggg acaacgaatt gcgcgccctc cgtcatcagc gaagcgcgcc cctgcggttt
      361 cggattgatg accttttcga acttggctgg cacctgcacc ctttgaacca atccgcgctt
      421 tggaatataa tgcccttttg gatttcgtgg cgaaatttgc atcgtgtggc ggaaaccgcg
      481 catccggctt tccggtgcgc tgaagttccc ctgcgcgaga gcgggagatt gatcatgtcg
      541 aaggcatcac ttttgctggt gacggtattg accggcctat cactcgccca gccggtcttt
      601 gctcaatcag aagatgacaa gcttggcaaa gttcatttcg agacgtcatg caacgcggaa
      661 gcgcaaaagc tgttcgaccg cgggatgctg tatcagcact cgttctggta ccgcgcctcg
      721 cagaaaatgt ttgaggacac gctaaaggca gatcccgcct gcagcatcgc ctattggggc
      781 accgccctta gcctgttgtg gaatccgcat acggcaccac cagcgaagaa tctcgcagaa
      841 ggtgcggccg cgatcgagaa agcgagaacg atcggattca agacggaccg cgaaaaaggt
      901 tatgttgacg cgctggcggc gatgtatact gattacgaca aggtggatca ccacgcccgc
      961 gtcgtagcct atgcgaaggc gatggaggcc ttggcgcaac gctatccgga cgatgacgag
     1021 gcgcagatcc actatgcgct tgcgctcaat acgtcagcct ccccggccga caagacctat
     1081 gcgaaccagc tcaagggcgc ggccatcctg gagccaatcg ccttgcggca gccgctgcat
     1141 ccgggcgtgg cgcactatct gatccatctc tacgactatc catcgatcgc agatcaaggc
     1201 atcgaggccg cgcggcgcta cgccaagatt gcgccggatg cgccgcatgc cctgcacatg
     1261 ccctcgcaca tcttcacccg ggtcggctat tggagcgagt cgatcgcatc caatgtcgca
     1321 tccgcgcgat ccgcaaaggg atcgagcgac ttctccgacg aactgcatgc gatggactac
     1381 ttggtctacg cctatctgca gctcggtcag gacgacaagg ccaaggccgt ggtcgacgag
     1441 atgggaaagg tcacgggctt cagcgagacc ttcattccgg ggccttacgc agaagcagcc
     1501 tcgcccgcac gctatgtgtt ggagcgtggc gactggaagg ccgcggaagc gctgacagtg
     1561 cgtccaagtc cgctcgccaa cgtgcaggcg atcagctatt tcgcccgcgc actcggagct
     1621 gcgcgggccg gcgaccccga agctgccaag cctgatatcg cgaggctcgg tgagcttcgc
     1681 gacaagcttc gcgaggcaaa agatgcctat tggtccgagc aggtcgatat ccaggccagg
     1741 atcgcctccg cctgggtgct ctacgccgag ggcaaaaagg atgacgcgct gaaggccatg
     1801 agcgatgccg ccgacgccga ggatcggacg gagaagcacc cggtgacgcc cggcacgccg
     1861 aaaccggcgc gtgagctcta tggcgagatg ctgctcgaca acggtcgcgc cgcggaagcg
     1921 ctaacggcct tcgaggcgac gctcaagaaa gagccgaatc gtcttggtgc ctatgccggc
     1981 gcggcgagag ctgcggcgca aatcggcgac accgcaaagg cacgcgaata ttttggcaag
     2041 atcgtagagc aagcgggcag cggggatagc acccgcaccg aggtcgcgga tgcgcgcaat
     2101 tatctcgccg ggcgctaaga gcgccccgcc gaaagcacac aacgccaccc gcgcaacatt
     2161 gtcgcttgca ctgagcctgc ttgcggcacc cgctggggcc gactcgctgc agattttcgg
     2221 ttattccggc tatctcggcg aatgggagtt gacggcaacc ctcaaagagg acggttcgac
     2281 gacaccgaaa aactattccg ggccgctctc gctgaaacac gttggcatct gtacccagga
     2341 cggcccggaa gaaaaaagcg gtgagatacg tgttcagatg ttgtcgtcct cgtcgcgcct
     2401 gcaagccacg ctttcagtgg acggggttga ctgcggctat caaggagtct tgtccgattt
     2461 ttacaacgga atcatgagtt gtcccggccg ggaagcggtg ccgctgaagc tttggatcaa
     2521 gtagtcgcgg ggaacgcaat cgctttcata cgggcccgat ccacgacccg accgttattt
     2581 agcacccagg ctggtggggt gtcatggcac cataaggccg aggcaatttc aacggcgctc
     2641 ccctgtgaca tgctccaaca acgccagcag tcccttaagc agcgcggttg gcgatggcgg
     2701 acagccggga atgtggagat cgaccggcac gacgtcggac acaccgccga cgcaggcgta
     2761 gctgcccttg aagatgccgc catcggccgc gcagccgccc actgcgacca cccatttcgg
     2821 atcgggcgtc gcatcgtagg tgcgcttcag cgcctcgcgc atgttcttgg ttaccggccc
     2881 cgtcaccatc aacacgtccg catggcgcgg cgacgcgaca aagcgcaggc cgaaccgctc
     2941 aaggtcgtag aaggcgttgg caagcgcgtg gatttccagc tcacaggcat tgcaggagcc
     3001 ggcgtcgacc tgacggatag acagcgagcg gccgagccgc gcctgtgccg ccttctccac
     3061 attcttcgca agttcggcga tcgcggcgtc gtcggcagcc ggtggctgtt cggttagcgg
     3121 accgcggatc aggctctcaa acagggttct tcgcatcttg cactcagaga tcagcgccgg
     3181 aatacgagca gttgaatgat ttgttgcaga gcggaaagtc ggcgatgatg ttgtcctcga
     3241 tcgcggcttc caggagcggc cactggaacc aggatgcgtc gcgcaggtgg cagcggtcga
     3301 tgcggccctc gctgtgaagc ctcaggcaga ccagcacatc gccgcgaaac gcttcggtca
     3361 gcgcgatgcc ttcgcaaggc gcgccgcccg gttttagcgc cacgttgaac gcacccggtg
     3421 ggagacgatc gaggatttga tcgatcaagg cgagagattg ttcaacctcg cggatgcgaa
     3481 tccagacgcg cgcgttgacg tcgccggcct caagcaccgg aacttcgaac gacagctcat
     3541 cgtacggcgc gtaagcaaga ttgcgccgcg cgtcgaaggc gcggcccgcg gcgcggccaa
     3601 cataaccgcc cgcgccgaat ttttgcgcat attcgcgctt gacgatgccg gtcgcaaccg
     3661 tgcggtcctg caacgatgcc gtattgtcgt agagttcgac gaggcgagga aagttctttc
     3721 ggatttcagc cgacaattcg cggatcgcag caggcccttc gggcgcgata tcgcgcgcga
     3781 cgccgccggg cacaacgaca tccatcatca gccggtgtcc gaagcaggta tcggccgcgc
     3841 gcaacacgcg ctcgcgcaaa attccggtct gtgcatgcat cagcgcgaag gaggcgtcgt
     3901 tacagatcgc accgatgtca ccgaaatgat tggcgagccg ctcaagttcc gccatcagcg
     3961 cgcgcagata tatggcacgc ggcggaacat tgatctgcaa ggcagcctcc acggcgcgcg
     4021 cgaaagcgaa cgaataggcc accgtgctgt cgcccgaggt gcggccagcc agcttcgccg
     4081 ccgcatcgag cgaggtgtcg gccatcaacg actcgatgcc cttgtggaca aagccgagcc
     4141 gttcttcgag ccgaaccaca tgctcgccgt tggcggcgaa tcggaaatga ccgggctcga
     4201 tgatcccggc gtgcaccgga ccgaccgcga tctggtgcag cccttcgcct tcgctcgaca
     4261 ggaattggta aggcccgcgg gcgcgcggcg cagcgggttt gccgaacggc gtcgacctcc
     4321 cccacacgcc gtgatcgagc caaggccgcg tatcggtcgc gccgacagcg tcaagaccga
     4381 agagatcgtg gatagtgcgc tcaagccgca gcgccggcgc gtggcgcgcg gcgacgcttg
     4441 gaaattttcc ggattcgcat tcaaggctga cagtggcaat ctcatccgtt gcgccgagca
     4501 gcaccagatg cacagccgat acctcgcccc agaagccgag cagggtgaca tcacctgcgg
     4561 cgagcaggtc gatagctttg agccagccct gctcgccgac cagaacgcgc ggccacggcc
     4621 ggtgagtggc gatcgcctct tgatacggaa tgagatcgag caggctcata gcatctcacc
     4681 ccaacaacct tgcgacattt gagaagcccg ccaccagcgc gggcggcaga tagatgccag
     4741 ccatcagaac cagcgcgagg tgcgtgaaca tcggcacgta agacgcttcg gcaggtttgg
     4801 tcagcccttt cggctcgccg aacgccacga cattgagtcg catgaacagc ccgccgagcg
     4861 cgatcaagat gccaaggacc aggattgccg tcagccaggg ctggcgggca aaggtcgaac
     4921 tcacgatcag aaactcactc atgaaaatgc cgagcggtgg caggccgacg atggcgacca
     4981 cgccgagcac gagcccccag ccgagtacgg ggttggtcac cgtcagtccg ccgatatcgg
     5041 ctatcttctg tgtgcccttg acctgcgcga tgtggccgac ggcgaagaag atcgcagact
     5101 tggtgagcga gtgcagggtc atgtgcaaca ggccggcaaa attcgcgagc gccccgccca
     5161 tgccgaaggc gaacacgatg atgcccatgt gctcgatcga ggaataggcg aacattcgct
     5221 tgatgtcgcg ccgacggtag agcatcagcg cggcgaagat taccgatatc aggcccatca
     5281 ccaccatcag cggtcccggg gcaatcgcgc cttcgcaggc cgccatgatc atcttgaagc
     5341 ggagcagcgc atagagcgcg acgttgagga gcaagcccga caacacggcc gagatcggcg
     5401 tcgggccttc ggcgtgggca tcggggagcc aggcatgcag cggcgcaagt ccgaccttgg
     5461 tgccataacc gagcaagagg aatacgaagg cgacgttgag cagcgccgga tcgaactgcg
     5521 tcgcgcgcgc gactagcact gtccagacca tcgcatccgt cccctcgccg atcaccggcc
     5581 gcgccgccat gtagaccaag atggtaccga acaacgcgag cgctatgccg acgctgccaa
     5641 gaatgaagta tttccaggcc gcttccagcg cctcgtgggt gcgatagatg ccgaccatca
     5701 gcacggtagt tagcgtcgcg acttcgacgg cgacccacat caaaccgatg ttgttggcga
     5761 ccagcgcaag gttcatcgcg
//
LOCUS       DATLJT010001436         2446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_355987,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001436 DATLJT010000000
VERSION     DATLJT010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355987"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /gene="napA"
                     /locus_tag="VM910_33005"
     CDS             <1..1495
                     /gene="napA"
                     /locus_tag="VM910_33005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HVI67360.1"
                     /translation="FVSDYTLEKAAEMSGVPLNRLEALAELYADPKTKVVSFWTMGFN
                     QHTRGVWCNNLVYNIHLLTGKISEPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVV
                     NNKEHRDKTEAIWKLPEGTIPDKPGYHAVLQSRMLKDGLLNAYWVQVNNNLQAGPNIN
                     EETYPGFRNPDNFIVVSEAYPSVSALAADLILPTAMWVEKEGAYGNAERRTQFWHQLV
                     TAPGESRSDLWQLMEFSKRFKIEEVWPEELLAKKPEYRGKTLFDVLYKNGQVDKFPQS
                     DIEEGYANDESKAFGFYVQKGLFEEYATFGRGHGHDLAPFDTYHRERGLRWPVVNGQE
                     TKWRFREGSDPFVRAGTNVQFYGYPDGKARIFALPYEPPAESPDNEYPFWLSTGRVLE
                     HWHSGTMTRRVPELYRAFPEAVCFMHPDDAQDAKLRRGDEVKVISRRGFMRARVETRG
                     RDRPPRGLVFVPWFDESKLINKVTLDATDPISLQTDFKKCAVRIEKV"
     gene            1501..1971
                     /locus_tag="VM910_33010"
     CDS             1501..1971
                     /locus_tag="VM910_33010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HVI67361.1"
                     /translation="MIRQARIVLLVAAIAAGTGTLAAQTLTSGLRGATPLNDEGPASP
                     MTPTRNTSEREMRNYPEQPPVIPHSIDGYQIDLNSNKCLTCHARARTGESQAPMVSIT
                     HFMDRDGQFLASISPRRFFCTECHVPQNVTNPPVSNDFVDVDTMLSRAAPGGRR"
     gene            1968..>2446
                     /locus_tag="VM910_33015"
     CDS             1968..>2446
                     /locus_tag="VM910_33015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HVI67362.1"
                     /translation="MSVLEKEGARPTFWRRAWTFLRELWDVLIRPSSVFGLGVLVLAG
                     FAAGVIFWGGFNTALELTNTEKFCTSCHEMHDNVFQELRSTIHFTNRSGVRATCPDCH
                     VPHNWTDKIARKMQASKEVWGHLFGFIDTREKFVDKRLELALHEWARFKANDSLECR"
BASE COUNT          505 a          777 c          720 g          444 t
ORIGIN      
        1 attcgtctcc gactacacgc tggagaaggc ggcggaaatg tcgggcgtgc cgctcaatcg
       61 ccttgaggcg ctcgccgagc tctacgccga tccgaagacc aaggtcgtca gcttttggac
      121 catgggcttc aaccagcata cccgcggcgt ctggtgcaat aacctcgtct acaacatcca
      181 tctgctgaca gggaaaatct ccgaacctgg caacagcccg ttctcgctga ccggccagcc
      241 gtcggcctgt gggaccgcac gggaggtcgg cacgttttcg catcgcctgc ccgccgacat
      301 ggtggtgaac aacaaggagc accgcgacaa gacggaggcg atctggaagc tgcctgaagg
      361 caccattccc gacaagccgg gctatcatgc cgtgctgcaa agccggatgc tcaaagacgg
      421 cctgctgaac gcctattggg tgcaggtcaa caataatctg caggcgggtc ccaacatcaa
      481 tgaggagact tatccgggct tccgcaatcc cgacaatttc atcgtggtca gcgaagcgta
      541 cccgtctgtc agcgcgctgg cggccgatct catcctgccg acggcgatgt gggtggaaaa
      601 ggaaggcgcc tacggcaatg ccgaacgccg cacgcagttc tggcaccagc tcgtcaccgc
      661 gcccggcgag tcgcgctcgg acctctggca gctcatggaa ttttccaagc gcttcaagat
      721 cgaggaagtc tggccggaag agctgctggc gaagaagccc gaatatcgcg gcaagacgct
      781 gttcgacgtt ctctacaaga acggccaggt cgacaagttc ccgcagtccg atatcgagga
      841 aggctacgcc aacgacgaat cgaaggcgtt cggcttttat gtgcaaaagg gcctgttcga
      901 ggaatacgcc accttcggcc ggggccacgg ccacgacctt gcgcctttcg acacctatca
      961 tcgcgaacgc ggcctgcgtt ggccggtcgt caacggccag gaaaccaagt ggcgcttccg
     1021 cgaaggcagc gaccccttcg tcagggcggg caccaacgtg cagttctatg gctaccccga
     1081 cggcaaggca cgcatcttcg cgctgcccta tgagccgccg gcggaatcgc ccgacaacga
     1141 atatccgttc tggctctcga cgggacgtgt gctggagcac tggcattccg gcaccatgac
     1201 gcgccgcgtg cccgagcttt atcgtgcatt ccccgaggcc gtctgcttca tgcatccgga
     1261 cgacgcgcag gacgccaaac tgcgccgtgg cgacgaggtc aaggtgatct cgcgccgtgg
     1321 cttcatgcgc gcccgcgtcg agacgcgcgg acgcgaccgg cccccgcgag gccttgtatt
     1381 cgtcccctgg ttcgacgagt cgaaactgat caacaaagtg accctggacg ccaccgatcc
     1441 gatctcgctg caaacggact tcaagaaatg cgccgtgcga atcgagaagg tgtgacggcc
     1501 atgatcagac aagcgagaat tgttctcctc gtcgccgcca tcgcggcggg taccggcacg
     1561 cttgcggcgc aaacgttgac ctccgggttg cgcggcgcga cgccgctgaa cgacgagggt
     1621 ccagcctcgc cgatgacgcc gacgcgcaat acctccgaac gcgagatgag gaactatccc
     1681 gagcagcccc ccgtcatccc gcattcgatc gacggttacc agatagatct gaatagcaac
     1741 aagtgcctga cctgccacgc ccgggcgcgc accggggaat cgcaggcacc aatggtttcg
     1801 atcacacact tcatggaccg tgacggccag tttctcgcct caatctcgcc gcgacgattt
     1861 ttctgcaccg aatgtcacgt cccccagaac gtaaccaatc cgccggtcag caatgacttt
     1921 gtcgacgtcg ataccatgct cagccgcgcc gcacccggtg gacggcgatg agtgtgcttg
     1981 aaaaagaggg cgcgcgccca accttttggc ggcgggcctg gacttttctc cgcgaactct
     2041 gggatgtact gatccgcccg agctcggtgt tcgggctcgg tgttttggtg ctcgcgggtt
     2101 ttgccgccgg cgtgatcttc tggggcggct tcaatacggc actcgaattg accaacaccg
     2161 aaaaattttg cacgagctgc cacgagatgc acgataacgt gtttcaggaa ttgagatcga
     2221 cgatccactt taccaatcgc tccggggtcc gggccacttg cccggattgt cacgttccgc
     2281 acaactggac ggacaaaatc gcgcgcaaga tgcaggcctc caaggaggtc tggggccatc
     2341 ttttcggctt catcgacacc cgcgaaaaat tcgtcgacaa gcggcttgaa ctggcgctgc
     2401 acgaatgggc gcgcttcaag gccaatgatt cgctggaatg ccgcaa
//
LOCUS       DATLJT010001437         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3342480,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001437 DATLJT010000000
VERSION     DATLJT010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342480"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..374)
                     /locus_tag="VM910_33020"
     CDS             complement(75..374)
                     /locus_tag="VM910_33020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HVI67363.1"
                     /translation="MIYVVATLTVKPETRAELIAAATACIKETRKEKGNIAYDLHESV
                     TDPAKLVFVEQWENAEALVPHRSAEHMKAFGRVAVKCMAAPPRIEVITPEKIEVR"
     gene            complement(422..2137)
                     /gene="ureC"
                     /locus_tag="VM910_33025"
     CDS             complement(422..2137)
                     /gene="ureC"
                     /locus_tag="VM910_33025"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HVI67364.1"
                     /translation="MSVKIKRSVYADMFGPTTGDRVRLADTDLIIEVEKDFTIYGEEV
                     KFGGGKVIRDGMGQSQVTNKQGAADTVITNALIVDHWGIVKADVAIKEGYINAIGKAG
                     NPDIQPGVTIVIGPGTDVIAGEGKILTAGGFDSHIHFICPQQIEHALMSGVTSMLGGG
                     TGPSHGTYATTCTPGPWHMGRMIQSFDAFPVNLGISGKGNASRPASLVEMIKAGACAL
                     KLHEDWGTTPAAIDTCLSVADDHDIQVMIHTDTLNESGFVEDTVKAFKGRTIHAFHTE
                     GAGGGHAPDIIKIAGLKNVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPTIAEDLAF
                     AESRIRKETIAAEDILHDLGALSMMSSDSQAMGRLGEVIIRTWQTADKMKKQRGSLPQ
                     DKGKDNDNFRVKRYIAKYTINPAIAHGVSKLIGSVEKGKLADLILWQPAFFGVKPDCV
                     IKGGSIVAAPMGDPNASIPTPQPVHYQPMFAAFGRSLTASSVVFTSKAAAAAGIGRKL
                     GIAKQLYAVQNTRGKISKKSMIHNDATPTIEVDPETYEVRADGELLTCAPAEVLPLAQ
                     RYFLF"
     gene            complement(2183..2722)
                     /locus_tag="VM910_33030"
     CDS             complement(2183..2722)
                     /locus_tag="VM910_33030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVI67365.1"
                     /translation="MLPAIRLLSEAAELAAHRHNGMARKGRGSEPYINHLAEVANLLA
                     TASDGEDAELVAAGWLHDTIEDTATTREELARKFSERVASLVVECTDDMSLPKAERRR
                     LQVVDAPHKSPGAKLIKIADKVSNIRARIFPHASREQRNELAEYVAWAEQVVNGCRGV
                     NAVLDTLFDEAVANARSTL"
     gene            complement(2727..3032)
                     /locus_tag="VM910_33035"
     CDS             complement(2727..3032)
                     /locus_tag="VM910_33035"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HVI67366.1"
                     /translation="MIPGELFIKDGEIELNAGRKTVTLAVSNTGDRPIQVGSHYHFFE
                     TNPALKFDRKKARGMRLDIAAGTAVRFEPGQTRDVQLVQLAGKRTIYGFRGDVMGKL"
     gene            complement(3046..3348)
                     /locus_tag="VM910_33040"
     CDS             complement(3046..3348)
                     /locus_tag="VM910_33040"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HVI67367.1"
                     /translation="MNLSPREKDKLLISMAAMVARRRLERGVKLNHPEAVAIISDFIV
                     EGARDGRSVAELMQAGAQVLTRAQVMEGIPEMIHDIQVEATFPDGTKLVTVHEPIR"
     gene            complement(3376..4206)
                     /locus_tag="VM910_33045"
     CDS             complement(3376..4206)
                     /locus_tag="VM910_33045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HVI67368.1"
                     /translation="MRSIGASSATFTANRARGAVCFDVRRVDGVTRRGSLHESGSLRV
                     RFPSPEGEGLSAMFVNTAGGIAGGDRFDIDVTVAEGAKLTLTTAAAEKVYRAQDETSE
                     FNIALKAAVGAHLAWLPQETILFDHARMSRRFDIDLADGASLLLCEIVVFGRAAMGET
                     MRHGSFVDRWRVRRGGRLVFAETVRLDGDVGEKLARAVIAKGGTAIGTLLIVPGDETL
                     VARMREAPASIDCEIGLSAWNGFAMARFCAQDAARLRADMQAVLARATSAPLPRLWLH
                     "
     gene            complement(4293..>4866)
                     /locus_tag="VM910_33050"
     CDS             complement(4293..>4866)
                     /locus_tag="VM910_33050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI67369.1"
                     /translation="SLLRAMVGQYPIASGSIVFDGVDITGLKPYERARRGIGFVPQGR
                     EIFPLLTVEENLKTGFGPLKRDDRYIPDDVFSLFPVLQSMLGRRGGDLSGGQQQQLAI
                     GRALVMRPKLLLLDEPTEGIQPSIIKDIGRAISYLRSLGNIAIVLVEQYLDFACELGD
                     NFAVMDRGAVKYSCDRSNLDPGEISRQMAL"
BASE COUNT          841 a         1516 c         1537 g          972 t
ORIGIN      
        1 ggctctccgg cttcatctga gtggtggcga cgacatagat catgaaaacg ctctcccgtc
       61 attttttgat ttatctagcg aacttcgatt ttttcgggcg tgatgacctc gatcctcggc
      121 ggcgccgcca tgcatttcac ggcgacgcgg ccgaaagcct tcatgtgttc ggccgagcga
      181 tgtggcacca gcgcctcggc attttcccac tgttcgacga acaccaattt ggccggatcc
      241 gtcacgctct catggaggtc ataagcgatg ttgcctttct ccttgcgcgt ctccttgatg
      301 caggcggttg cagcggcgat cagctcggcg cgcgtttcgg gcttcacggt caacgtggca
      361 acgacgtaaa tcatggacaa tcctcccggc acctggtttt ggtttgatac cgggaccgac
      421 tttagaacag gaaatagcgc tgcgccaagg gcagcacttc ggccggcgcg caggtcagga
      481 gttcgccgtc ggcgcggact tcgtatgttt cgggatcgac ctcgatcgtg ggcgtcgcgt
      541 cgttgtggat catgctcttc ttggagatct tgccgcgcgt gttttgcact gcataaagct
      601 gcttggcgat cccaagcttg cgcccgatcc cggcagcggc ggcggccttg gacgtgaaga
      661 ccaccgagga cgccgtgagc gatctgccga atgccgcgaa catcggctga tagtgcacag
      721 gctgcggcgt cgggatcgac gcgttcggat cgcccatcgg ggctgcgacg atcgagccac
      781 ccttgatgac gcaatcgggc ttgacgccga aaaacgcggg ctgccacagg atgagatcgg
      841 cgagcttgcc cttctccacc gatccgatca gtttcgagac accgtgagcg attgcggggt
      901 tgatggtgta tttggcgatg tagcgcttga cgcggaaatt gtcgttgtcc ttgcccttgt
      961 cctgcggcag cgaacctcgc tgcttcttca tcttgtcggc ggtctgccag gtacggatga
     1021 tgacctcccc gagccggccc atggcctggg agtccgatga catcatcgag agcgcgccga
     1081 gatcgtgcaa aatatcttcc gccgcgatcg tctccttgcg gatgcggctt tcggcaaacg
     1141 cgaggtcctc ggcgatggtg ggatcgaggt ggtggcacac catcagcatg tccagatgct
     1201 cgtcgatggt gttgcgcgtg aaggggcgcg tcggattggt ggaggacggc aatacgttct
     1261 tcaagccggc gatcttgatg atatccggcg catggccgcc gccggcgcct tcggtgtgaa
     1321 aggcatggat ggtgcggccc ttgaaggcct tcaccgtatc ctcgacgaag cccgactcgt
     1381 tcagcgtgtc ggtgtggatc atgacctgaa tgtcgtggtc gtcggcgacc gagaggcaag
     1441 tatcgattgc agccggcgtc gtgccccagt cctcgtgcag cttcagcgca caggcgcccg
     1501 ccttgatcat ctcgaccagc gaggccgggc gcgaggcgtt gcccttgccg gagatgccga
     1561 gattgacggg aaaggcgtcg aacgactgga tcatgcgccc catgtgccag gggccgggcg
     1621 tgcaggtcgt ggcataagtg ccgtgcgagg ggcccgtgcc gccgccaagc atcgaggtga
     1681 cgccgctcat cagcgcgtgc tcgatctgct gcgggcagat gaaatggata tggctgtcga
     1741 agccgccggc ggtgaggatt tttccctcgc ctgcgatcac gtcggtgccc gggccaatga
     1801 cgatggtgac gcccggctgg atgtcgggat tgccggcctt gccgatggca ttgatatagc
     1861 cctccttgat cgcgacatcg gccttcacga taccccagtg atcgacgatc agcgcattgg
     1921 tgatgacggt gtccgcggcg ccctgcttgt tggtgacctg cgactggccc atgccgtcgc
     1981 ggatcacctt gccgccgccg aatttcacct cctcgccata gatcgtgaaa tccttctcca
     2041 cctcgatgat gaggtcggtg tccgccagcc gtacgcggtc gccggtggtc gggccgaaca
     2101 tgtcggcata gactgaacgt ttgatcttga cggacatcgg acccccactc gaaatttcag
     2161 ttgtgccagt cggcaatcgc cctcacagcg tgctcctggc attggcaacg gcctcgtcaa
     2221 agagagtgtc gagcaccgca ttgacgccgc ggcagccgtt cacgacctgc tccgcccagg
     2281 cgacatactc ggcaagctcg ttgcgctgct cgcggctcgc atgcgggaag atgcgcgcgc
     2341 gaatgttgct gaccttgtcg gcgatcttga tcagttttgc ccctggcgat ttatggggcg
     2401 cgtcgaccac ctgcagccgc cgacgctcgg ccttgggcaa gctcatgtcg tcggtgcact
     2461 cgaccacgag ggaggcgacc cgttcggaga atttccgtgc cagctcctcg cgcgtggtcg
     2521 cggtgtcctc gatcgtatca tgcagccagc ccgccgcgac aagttcggcg tcctcgccat
     2581 cgctcgctgt cgcaagcaga ttggcgacct cggcgagatg attgatatag ggttcgctgc
     2641 cgcgcccctt gcgtgccatg ccgttgtggc gatgtgcggc aagctcggcg gcttcggaca
     2701 ggaggcggat ggccggcagc atggcctcac agctttccca tcacgtcgcc gcgaaaaccg
     2761 tagatggtgc gcttgccggc gagctgcaca agctgcacgt cgcgggtctg tccgggctcg
     2821 aagcgcaccg cagtgccggc tgcgatgtcg agccgcatgc cgcgcgcctt tttgcgatcg
     2881 aacttcagtg cgggattggt ttcgaagaaa tggtagtgcg agccgacctg gatcggacgg
     2941 tcgccggtgt tggaaaccgc caaggttacg gtcttgcggc cggcgttgag ctcgatctcg
     3001 ccgtccttga tgaagagttc gcccgggatc atcttggctc ctgtgtcacc tgatcggttc
     3061 atgcacggtg acgagttttg ttccatccgg gaaggtcgct tcgacctgga tgtcgtggat
     3121 catttcgggg attccctcca tcacctgcgc ccgggtcaag acctgcgcgc ccgcctgcat
     3181 caattcggcg acgctacggc cgtcacgcgc tccctcgacg atgaagtccg aaatgatagc
     3241 gaccgcctcc ggatggttga gcttgacgcc tcgctccagc cggcgtcgcg ccaccatggc
     3301 cgccatcgaa atcagaagct tgtccttttc gcggggtgag agattcatga gggttttctc
     3361 ttgatttgag ccgatctagt gcagccataa ccgcggcagc ggcgccgacg tggcgcgcgc
     3421 aagcacagcc tgcatatccg cgcgcagtcg cgcggcatct tgggcacaga agcgcgccat
     3481 tgcaaatcca ttccaggcgg aaagcccgat ctcgcagtcg atggatgccg gcgcctcgcg
     3541 catgcgcgca accagggtct catcccccgg cacgatcagc agcgttccga tggcggtgcc
     3601 gcctttggcg atcacggcgc gtgcgagctt ctcaccgaca tcgccgtcaa gccggaccgt
     3661 ttcggcgaat acgagcctgc cgcctcgcct cacccgccag cggtccacga agctgccatg
     3721 ccgcatggtc tcgcccatcg cggcacggcc gaacacgacg atttcgcaga gcagaagcga
     3781 ggctccatcg gcgagatcga tatcgaagcg gcgcgacatc cgcgcgtggt cgaacaaaat
     3841 ggtctcctgc ggcagccatg cgagatgtgc accaacggcc gctttcaggg caatgttgaa
     3901 ctcggaggtc tcgtcctggg cgcgatagac cttttccgcc gccgctgtgg tgagcgtcag
     3961 cttcgcgccc tcagccacgg tgacatcgat atcgaagcgg tcgccgcctg cgattccgcc
     4021 tgcggtgttc acgaacatcg ccgacagccc ctcgccttcg ggcgacggaa atcgcaccct
     4081 gagcgagccc gattcgtgca ggctcccgcg ccgggtgaca ccgtcgacac gcctgacatc
     4141 gaagcaaacc gcgccacgag cgcggttggc cgtaaaagtc gccgacgacg cgccgatgga
     4201 gcggatttga ggttccctac ccatttgccg cgatcatcct aacgcccgtc cccgcccgcg
     4261 cagcgcagcc tcaagccggc cgtgccgaaa acctaaagcg ccatctggcg gctgatttcg
     4321 cccggatcga gattggagcg atcgcaggaa tatttcactg ccccgcgatc catcacggcg
     4381 aaattgtcgc cgagctcgca ggcaaagtcg agatattgtt cgacgagcac gatggcgatg
     4441 ttgccgaggc tgcgcagata ggagatcgcg cggccgatgt ccttgatgat cgacggctgg
     4501 atgccttcgg tcggttcatc gagcagaagc aattttggcc gcatcaccag cgcgcgcccg
     4561 atcgcaagct gctgctgctg gccgccggac aggtcgccgc cgcgccgccc gagcatggat
     4621 tgcagcaccg gaaacaggga gaagacgtca tcggggatat agcggtcgtc tcgcttgagc
     4681 ggtccgaaac ccgtcttcag gttttcttcc acggtcagca gggggaatat ctcgcggcct
     4741 tgcggcacga agccgatgcc gcgccgcgcc cgctcgtaag gcttcaggcc ggtgatgtcg
     4801 acgccgtcaa agacgatcga gccggaagcg atcgggtatt gaccgaccat ggcgcgaagc
     4861 agcgag
//
LOCUS       DATLJT010001438         5147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3342559,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001438 DATLJT010000000
VERSION     DATLJT010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342559"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(498..1772)
                     /locus_tag="VM910_33055"
     CDS             complement(498..1772)
                     /locus_tag="VM910_33055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67370.1"
                     /translation="MAIAAGATDLPVAWWKEPTKDQWLAWIAAWLGWTLDAFDFTIFL
                     LIMAPIAKEFGVSVTAVAAVLTFTLWLRFVGAVGSGWLADRIGRKTPLMLSILWYSAC
                     NFIAGFSPTFSFLFVVRALLGIGMGAEWPAGASLAMESWPARSRGLMSGVLQGSWSLG
                     FALSTVAYWLLFDAIGWRGLLWLGILPAFVCVYIRYYVKEPAVWVENRKRQREQRKEV
                     RAPLLTIFKPALLGNTLTACWWIASSLMVYYSIYALFATWLQTEFKLAPAVVATPILL
                     SNLASFGGMGFWGWASDRIGRRWAMIIPATIGCVLALAYLLTNNQNWIIVGFVIQGLF
                     LGAIYQFPSYLSERFPTEVRATGSAFCYHVGVICAGVVPPVISYFAVEQHMGFAMPML
                     ISTMVTATSVVIALLISPETKGKVFVSDLMRH"
     gene            complement(2005..>5147)
                     /locus_tag="VM910_33060"
     CDS             complement(2005..>5147)
                     /locus_tag="VM910_33060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67371.1"
                     /translation="GSTALSARLDPEDMRAVIDAYHAACARITRTYDGFLAEFRGDGI
                     LAYFGYPFAHEDDAERSVRAGLDIIAAVAQLETRAAEPLAVRIGIASGVVVVGDLSRE
                     GALREHAVVGETPNLAARVQALAEPGTVVVAASTRRLLGDLFRLRDLGRHEVKGIAEP
                     VAAWGVEGVSASESRFEAVRAARLTDLIGREDELGFLLERQRLAWKGEGQIVLISGEP
                     GIGKSRLAAALAERIASEPHTRLRYQCSPYRTNSALRPFIAQLERAAGFKADDTSEQR
                     LDKLEAVLAMSASRIEGVAPLFAALLSIPFGERYPPLALSPTQQRRRTLAALLDQFEG
                     LARRQPILLVFEDANWADATSLELLDLTVERVRQLPVLALFTFRPEFEPPWIGLPNVG
                     TLTLGRLDRNDVESMVTRVTGGRVPPPEVMKQIVAKTDGNPLFVEELTKTVLEAGILV
                     EDAEGYRLDGPLPPLAIPATLQDSLMARLDRLAPVKEISQIGAAIGREFSYSLLRTLV
                     GRDETALKGALAQLEHAEIVFRRGEPAEAIYSFKHALVRDAAYESLLKSRRQVLHGRI
                     AETLRNKSPAIADTEPEVIAHHLTHAGLAEAAVEWWGKAGERAMEGSAYMEAIAHLEK
                     GIGLAKGLADEPAQRLIRLRLQITYAYALLHARGQTMPKTIAAFARARELATGVEDIT
                     ERASAYYGLWAGSLLRADLPRMREVADVFVREAQRLSESPEIGVAHRVFGITCWFNGD
                     YVSARVHLEQAVAAYDHERDCHLASRFGYDPGVVAMFFLGLVLWSLGEVDRAARLAEE
                     ALSLALRSDTPTVACAHCYTCIFAAIRRKSDRSTEHASALLTLAREHEMPTWLMHGTF
                     LHGWARCCSSDREAGTEMREGLALARKLDIGLWLSLYGTLVAEVEADAGPVEAGLATL
                     DAQLLEVEQTEERWFVAEMHRVRGELLLKVQPPDVAAAESALLRALEIARSQHARTFE
                     LRAALSLAKLYQTTDRNHAACELLVAALAGFSASPELPEVEEAQRLLTETVQRDGASP
                     GLSATWWATTRKEHRA"
BASE COUNT         1054 a         1637 c         1560 g          896 t
ORIGIN      
        1 tcagaccgcg aatgagcttg gtgctccggt cgcttcttcc atacccgaac agttgccagg
       61 gcacgagccc gcatcagcaa ggaagggtta cgaagatgct ctcgttttgc gcaatcgacg
      121 acatcgtttg ggccgatcgt ctgagcggtc ggcatagcgt gacgaccgca cggtagccgt
      181 tacgctggtc gatttggccg agtcagggcc gtagccgcac gcagcgcggg ggcgcgaagc
      241 gccagccttg acgcggccaa agcgatcagc gatccccttg cgacaaaatc acagttcacc
      301 tcgtcggcgc gctgctgtag aaaccaaggc acgtcgaggc cgagcgcctt ggcggtcttg
      361 aggttgatga ccagctcagt accccatcct ccagttcagc ccgtcgctct cgctcaaaat
      421 gacaggggcc atttgcattc gaccatcgca tcgtccgatc taggaatcat ctattctgaa
      481 tagaattttt tcggacacta gtgcctcatc aagtcggaaa cgaacacttt gcctttcgtt
      541 tccggactga ttaacaatgc aatgacaacg cttgtagcgg taaccattgt gctgatcagc
      601 ataggcatcg caaaacccat gtgctgctcc actgcgaagt acgaaatcac cggcgggacg
      661 acccccgcgc agatcacgcc cacgtggtaa cagaacgcac tccccgtggc gcgcacctcg
      721 gttgggaagc gctcgctcaa gtagcttggg aactgataaa tggccccaag gaacaggcct
      781 tggatgacaa agccgacgat gatccagttc tggttattag tgagcaagta ggccagcgcc
      841 aagacgcaac cgattgttgc ggggatgatc atcgcccagc ggcgaccaat gcggtcggag
      901 gcccagcccc agaaccccat gccgccgaat gaggcaaggt tggatagcaa aatcggtgtg
      961 gcgacaaccg caggagcgag cttgaattcg gtctgtaacc acgtagcaaa cagcgcgtaa
     1021 atcgaatagt agaccataag ggagcttgca atccaccagc acgcggtcaa cgtgttaccg
     1081 agaagtgcag gcttaaagat ggtaagaagc ggcgcacgca cctcctttct ttgttcacgt
     1141 tgccgcttgc ggttctccac ccaaactgca ggctctttca cgtagtatcg gatgtacaca
     1201 catacgaacg ccggcagaat gccaagccaa agaagtccgc gccagccgat cgcgtcgaag
     1261 agcaaccaat aggccacggt agagagcgca aagccgagac tccacgaccc ctgcaagaca
     1321 ccgctcatca agccgcgtga gcgggccggc cacgactcca tcgcaagact tgcgccagcg
     1381 ggccattccg cgcccattcc gattccgagc aaggcccgaa cgacaaacag gaaagagaag
     1441 gttggtgaaa aaccggcaat gaaattgcag gccgaatacc acaagatgga gagcatcagg
     1501 ggtgtcttgc gaccgatccg gtcggccagc cagccggagc cgactgcccc gacgaaacgc
     1561 agccaaagcg tgaaggtgag caccgccgct accgcggtta ccgaaacgcc gaactccttg
     1621 gctatcggcg ccatgataag caggaagatt gtgaaatcga atgcgtcaag cgtccacccg
     1681 agccatgcgg caatccaagc caaccactga tccttggtcg gttctttcca ccaagcaaca
     1741 ggcaaatccg tagcaccggc agctatcgcc attgcatttc ctccctcgct ttgcggagat
     1801 caaagctggg taacctatga ctggtcccta cgacgtcccc agtctgcggc tgtcgcgtgc
     1861 catgcatcgg cctaacgtgc ttcctgtcgt ccgcgctata cgaacaggag ggcggagacc
     1921 ctgcaggcgc tggccgacgc ctcgagcgcg tggcatgccg acggcgcgcc ggacgctgac
     1981 acgcgatgac cgagcacaac ctgatcaagc gcgatgctcc ttacgggtag ttgcccacca
     2041 tgtcgccgac aaaccggggc tcgcaccgtc tcgttggacc gtctccgtca agaggcgttg
     2101 cgcttcctcg acctctggca gctccggact cgcgctgaag cccgcgaggg cggcgacgag
     2161 cagctcgcaa gcggcatgat ttcggtccgt ggtttgatag agttttgcaa gtgatagggc
     2221 tgcgcgcaac tcgaacgtgc gcgcatgttg gctgcgagca atctcgaggg cgcgcagcaa
     2281 cgccgattcg gcggccgcaa catcgggcgg ttgcaccttc agaagaagtt caccgcgcac
     2341 ccgatgcatc tcagccacga accagcgctc ctcggtctgc tcgacctcga gcagttgcgc
     2401 atccaaggtc gcaagcccgg cctccacggg gcccgcgtct gcctccacct ccgctaccag
     2461 ggtaccgtaa agcgagagcc acaatccaat gtccaactta cgtgccagcg ccaacccttc
     2521 gcgcatctct gtgcctgcct cacgatcgct gctgcaacag cgcgcccagc catgaaggaa
     2581 agtgccatgc atcagccatg tgggcatttc gtgctcgcgc gccagcgtga gaagagcgct
     2641 cgcatgttca gtggatcggt cggatttacg gcggattgcc gcgaaaatac acgtgtagca
     2701 atgcgcgcat gctacggtcg gagtgtcgct tcgcagggcc aagctgagtg cttcctcagc
     2761 aaggcgagcc gcccgatcga cttctcccag agaccagagc accagaccga ggaagaacat
     2821 ggcaacaacg ccgggatcgt agccaaagcg ggaggcaaga tggcaatcgc gctcgtggtc
     2881 atatgctgca acagcctgtt cgaggtgtac gcgcgcgcta acgtaatcgc cgttgaacca
     2941 gcaagttatg ccaaagacac gatgcgccac gccgatctct ggcgattcgg acaagcgctg
     3001 cgcttctctt acaaagacat ctgccacctc gcgcatgcgc gggaggtctg ctcgcaaaag
     3061 gcttccggcc catagaccgt aataggccga ggctcgttcc gtaatatctt cgaccccggt
     3121 tgcaagctcc cgggcgcgcg cgaaagcagc aattgttttt ggcatggtct gcccgcgagc
     3181 atgcagcagc gcataggcat aagtaatctg gagccgtaac cgtatgaggc gttgtgcagg
     3241 ctcgtcggcc agtcctttgg ccaggccaat ccctttttcg agatgcgcaa tggcttccat
     3301 gtaggcggac ccttccatcg cccgctcgcc cgcttttccc caccattcga ccgcagcttc
     3361 cgcgaggcct gcatgcgtca aatgatgcgc gatgacctcc ggctcggtgt ctgcaatcgc
     3421 tggggacttg ttgcgcaggg tctcggcgat gcgcccgtgc agcacttggc gacgactctt
     3481 gagcaggctc tcataggcag cgtcccgcac tagcgcgtgc ttgaagctgt agatggcttc
     3541 cgccggctcg ccgcggcgga acacgatttc cgcatgctcg agctgggcga gtgcaccctt
     3601 caatgcggtc tcgtcgcggc ccaccaacgt gcgcagcagg gaataggaga actcgcgccc
     3661 gatggcggca ccgatctgac tgatctcctt tactggcgcc agacggtcga gccgcgccat
     3721 cagagaatct tggagcgttg ccgggatggc gagcggcggc aggggtccat cgagccgata
     3781 gccctcggcg tcctcgacca ggatgcccgc ctccagcacc gtcttggtga gttcttcgac
     3841 gaatagcggg ttcccgtcgg tcttggcgac gatctgtttc atcacttcgg ggggcggcac
     3901 gcggccgccg gtcacccgcg tgaccatgct ttcaacgtcg ttgcggtcga ggcgaccgag
     3961 cgtcaaggtg ccgacgttgg gcagaccaat ccagggcggc tcgaactccg gtcggaaggt
     4021 gaacagtgca aggaccggta gttgacgcac ccgctcgacc gtcagatcaa gtagctcgag
     4081 ggaggtggca tccgcccagt tcgcatcctc aaacacgagc aggatgggct gccggcgggc
     4141 gagaccctcg aattgatcga gcagtgccgc gagcgtccgg cggcgctgct gcgtggggct
     4201 gagcgccagc ggcggatacc gctcgccgaa cgggatcgac agcagcgcgg caaaaagcgg
     4261 cgctacgcct tcaatccgcg atgcgctcat ggcgaggacc gcttccaact tgtcgaggcg
     4321 ttgctcggag gtatcgtcgg ccttgaaccc tgccgcccgc tccagctggg cgatgaacgg
     4381 acgaagcgcg ctgttggtgc ggtagggcga gcattggtaa cgcagccgcg tgtgcggctc
     4441 gctggcgatg cgctcggcga gcgccgccgc aagccgggat ttgccgatcc ccggctcgcc
     4501 cgagatcagc acgatctgcc cttcgccctt ccaggcgagg cgttggcgtt ccagcaggaa
     4561 accgagctcg tcctcgcggc cgatgagatc ggtcagacgc gccgcgcgga ccgcctcgaa
     4621 gcggctctcg gaagccgata cgccctcaac cccccatgcc gcgaccggct cggcaatacc
     4681 tttgacctcg tgccgcccga ggtcgcgcag acggaacaga tcgccgagca gccggcgcgt
     4741 ggaggcggcg acgacgaccg ttccgggctc ggccaaggcc tgaacgcgcg ctgcaaggtt
     4801 cggcgtctcc ccaaccacgg cgtgctcgcg caacgcaccc tcgcggctga ggtcgccgac
     4861 caccacgacg ccgctcgcga tgccgatgcg gaccgcgagc ggttccgcgg cgcgcgtctc
     4921 gagttgggca acggcggcaa tgatatcgag ccccgcccgc acggatcgct cggcatcgtc
     4981 ttcgtgcgcg aagggatagc cgaaataggc caatattccg tccccccgaa actcggcgag
     5041 gaagccgtca taggttcgcg tgatgcgggc gcaggcggcg tgataggcat cgatcacagc
     5101 tcgcatgtcc tccggatcga ggcgggccga tagggcagtc gagccga
//
LOCUS       DATLJT010001439         1829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2883620,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001439 DATLJT010000000
VERSION     DATLJT010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883620"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VM910_33065"
     CDS             <1..156
                     /locus_tag="VM910_33065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HVI67372.1"
                     /translation="SLKYRADPGAPPIPVGGPTNDLIAVAVGFIAYLALAFAFHPVVI
                     GVPVIGN"
     gene            160..984
                     /gene="panB"
                     /locus_tag="VM910_33070"
     CDS             160..984
                     /gene="panB"
                     /locus_tag="VM910_33070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVI67373.1"
                     /translation="MSVQSSIKRKTAPDIRARKGKEPIVVLTSYHAHTAVVVDRHCDV
                     ILVGDSLGNVMHGFETTVPVTLDMMILQGRAVMRASSQALVVVDLPFGSYEASREQAF
                     QSAVRVMKETQCGAVKLEGGARMAETIAFLVERGIPVMGHVGLTPQSINALGSFRAQG
                     RDEGSWEPIERDARAVSDAGAFAVVIEALAEPLARKITETISIPTIGIGGSAACDGQV
                     LVLEDMLGLSPRTPKFVRRYADLLPAIEAAVEGYARDVRARAFPGPEHVYGMKAKG"
     gene            989..>1829
                     /locus_tag="VM910_33075"
     CDS             989..>1829
                     /locus_tag="VM910_33075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVI67374.1"
                     /translation="MDWSNYTNPAMRLEARFGDRMVPVFCERPNGIWAMVEAAVARNG
                     DGEALICGAARMTWREVAQKSAQIAAGLRAKGLKRGDRIALLLGNRLEFVLAMFAAAH
                     QGLITVLLSNRQQKPEIAYVLENCGAKLLIHEASLSDRLPDARDVPDLEHRIAVDDDA
                     RLSRFAEIADRPPLAAATQVGEEDTAMILYTSGTTGRPKGAMLAHCNMVHSAMLYETC
                     MGLTPKDRSIAAVPLGHVTGVVVNIMSMARCAGALIIMAEFKAAEYLKVAARERITYT
                     VMVP"
BASE COUNT          321 a          561 c          610 g          337 t
ORIGIN      
        1 tcgctgaagt atcgtgctga ccccggtgcc ccgccaattc ccgtcggcgg ccccaccaac
       61 gacctgatcg cggttgcggt cgggttcatc gcctaccttg cgctcgcctt tgccttccac
      121 ccggtggtga tcggcgtgcc cgtcattggg aactgagaca tgtccgtgca atcttccatc
      181 aagcgcaaga ctgcgcctga cattcgcgct cgcaagggca aagagccgat cgtggtgctg
      241 acttcctatc acgcgcatac cgcagtagtg gtcgaccgcc attgcgatgt gatcctggtc
      301 ggggattcgc tcggcaatgt catgcacggc ttcgagacca cggtgccggt gacgctcgac
      361 atgatgatcc tgcagggccg tgcggtgatg cgcgcctcaa gtcaagcgct ggtcgtggtc
      421 gacttgccct tcggctcgta tgaagcgtcc agggaacagg cgtttcaatc ggcggtgcgg
      481 gtcatgaaag agacgcaatg cggcgcggtc aagctcgagg gcggcgcgcg gatggcggag
      541 acaattgcct ttctggttga gcgcggcatc cctgtgatgg gccatgtcgg cctgacgccg
      601 caatcgatca acgcgctcgg ctcgtttcgt gcgcaggggc gcgacgaggg aagctgggag
      661 ccgatcgaac gtgatgcccg tgccgtgtcg gacgcaggcg cgttcgccgt ggtgatcgag
      721 gctctcgccg agccgctggc gcggaagatc acggaaacga tctccattcc cacgatcggc
      781 atcggtggca gcgcggcctg cgacggacag gtcctcgtgc tggaggacat gctgggcctg
      841 tcgccccgta cgcccaaatt cgtgcgtcgc tacgcggacc ttttacccgc aatcgaagcg
      901 gcggttgaag gttatgcacg agacgtccgc gcccgtgcgt ttcccgggcc ggagcatgtc
      961 tacggcatga aagcaaaagg gtagcgagat ggactggtcg aattacacga atccggcaat
     1021 gcggctcgaa gcgcgcttcg gtgatcgtat ggtgccggtg ttttgcgagc gaccgaacgg
     1081 catctgggcg atggtggagg cggcagtcgc ccgcaacgga gacggcgaag cgctgatctg
     1141 cggcgcggcg cggatgacat ggcgcgaggt cgcgcaaaaa tccgcgcaga tcgcagccgg
     1201 cctgcgcgcg aaagggctca agcgcggcga ccgcatcgcg ctcttgctcg gcaatcgcct
     1261 ggagttcgtt ctggcaatgt ttgccgcggc ccatcagggc ctgatcacgg tgctgctttc
     1321 gaatcggcag cagaagcccg agatcgccta tgtgctggaa aattgcggcg caaagctcct
     1381 gattcacgag gcgtcgcttt cggaccgtct gcccgatgcg cgggatgttc ccgatctcga
     1441 gcaccggatc gccgtcgacg atgacgcgcg gctgtcgcga tttgccgaga tcgccgatcg
     1501 cccgccgctt gccgccgcca cgcaagtcgg tgaagaggac accgccatga tcctctacac
     1561 ctcgggcacg acagggcggc cgaagggcgc gatgctcgcg cattgcaaca tggtccattc
     1621 ggcgatgctc tacgaaacct gcatgggact gacgccaaag gatcgctcta ttgcagccgt
     1681 gccgctcggc catgtgaccg gcgtcgtcgt caacatcatg agcatggcgc gctgcgccgg
     1741 cgcgctgatc atcatggccg agttcaaggc ggccgaatat ctgaaggtcg cagcgcgcga
     1801 gcgcatcacc tacacggtga tggtgccgg
//
LOCUS       DATLJT010001440         1925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2194657,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001440 DATLJT010000000
VERSION     DATLJT010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194657"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..838
                     /locus_tag="VM910_33080"
     CDS             23..838
                     /locus_tag="VM910_33080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67375.1"
                     /translation="MPESLEEVVAVYGHTFFKLKVSGSADADLQRLIEIAAVLEGIAG
                     AYHVSLDGNEQYDDLNALLDLWRRMQASPRLKRLIESVLFIEQPINRKHALDRDVSAL
                     SGIKPVIIDESDAELGAFPSARALGYSGVSSKCCKGLYKSVLNAARCGMWNAEAGGVR
                     YFMSGEDLTTQAGLAVQQDLALVNLLGLTHVERNGHHYVNGMAGLPQREQDGFLAAHP
                     DLYEHRRGAARVKIRNGRLAIGSLASMGFASSAYPDWAMLRPLSLALQRTASA"
     gene            1060..>1925
                     /locus_tag="VM910_33085"
     CDS             1060..>1925
                     /locus_tag="VM910_33085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI67376.1"
                     /translation="MKIARVNIYLIASGRLHPVIVEILTDEGVSGIGEAAIAYGLGGT
                     AAAGMVKDLSERMLIGRDPFRIEEIWSEMYVHSFWAKGGGPIVFAAISAIEQALWDVK
                     GKALGIPVYEFLGGRMRDRVHVYANGWYGAATTPTELAKAAERPLRDGYDALKCYPLA
                     YPDANGLCHVSRRMVDRDFANLAFAKIKTLRDAVGPDIAIMLDLSGGLTTDETIRLCQ
                     RFEELNILWIEEPADPFDVGALKRISDRVDIPIAVGERIYTRHGFRKIFEPHAADIVQ
                     PDAGNTGGIMET"
BASE COUNT          362 a          591 c          618 g          354 t
ORIGIN      
        1 tccccgcaaa atcaacgacg gattgccgga atcgctggaa gaggtcgttg ccgtctacgg
       61 ccacactttt ttcaagctca aggtcagcgg ttcggccgac gccgacctgc agcgcctcat
      121 cgaaattgcg gcggtgctgg aaggcatcgc cggggcctac catgtctcgc tggacggtaa
      181 cgagcagtat gatgatctta atgccctgct cgatctgtgg cggcgtatgc aagcgtcgcc
      241 ccgactgaag cgcctgattg aaagcgtctt gttcatcgag cagcccatca accgcaagca
      301 tgcgctcgat agggacgtgt cggcactgtc gggcatcaag ccggtgatca tagacgagtc
      361 cgatgccgag ctcggcgcct tcccatctgc ccgcgcgctc ggctacagcg gagtgtcgag
      421 caaatgctgc aaggggctct acaaatccgt cctcaacgcg gcccgctgcg gaatgtggaa
      481 cgccgaggcc ggcggcgtgc gctacttcat gtccggtgag gatctcacca cacaggccgg
      541 gctggctgtg cagcaggatc tcgcactggt caatctgctc gggctgacgc acgtagagcg
      601 caacggccat cattacgtca acggcatggc cggattaccc caacgcgaac aagacggctt
      661 tctcgccgcg caccccgacc tctacgagca ccgccggggc gcggcgcggg tgaagatccg
      721 caacggacgg cttgcgatcg gctcactcgc gagtatgggg tttgcctcca gtgcgtatcc
      781 cgattgggcg atgctcaggc cgctctctct ggcactacag cggacggcaa gcgcatgacg
      841 acggagctcg cgcgcggagc gccggtgccc ggggagttct tgcaccgcgt ttgaccagtc
      901 cggtttgggc gccctacccg ttccataacc tgtcattctg cgaacggctt ggatgcacgt
      961 gccccgcctt gggcggcgct caggctgctt gtttctcctc gcgaagcgga ttgtggtgca
     1021 gagcttgaat tcaaagctag caactgccgg gaaccagcca tgaagatcgc gcgcgtcaac
     1081 atttatctga tcgcgagcgg aaggctgcac ccggtcatcg tcgagatcct taccgacgag
     1141 ggcgtcagcg gcatcggcga agcggccatc gcctacgggc tcggcggcac cgccgcggcg
     1201 ggcatggtca aggatttgag cgagcgcatg ctcatcggcc gcgatccgtt ccgtatcgag
     1261 gaaatctggt ccgagatgta tgtccactcg ttctgggcca aggggggcgg accgattgtg
     1321 ttcgccgcca tcagcgcgat cgagcaggcg ctgtgggacg tcaagggtaa ggcgctcggc
     1381 atcccggtct acgaatttct cggcggcagg atgcgcgacc gcgtgcacgt atatgcgaac
     1441 ggctggtacg gcgcggctac gacgcccacc gagcttgcca aggcggcgga acgcccgctg
     1501 cgcgacgggt acgatgcgtt gaagtgctac ccgctcgcct atccggacgc aaacggattg
     1561 tgtcacgtgt cgcgacggat ggtcgatcga gattttgcca atcttgcgtt tgccaagatc
     1621 aagactttgc gcgacgcagt gggtcccgac atcgccatca tgctcgacct gagcggtggc
     1681 ctcaccacgg acgagacgat ccggctgtgc cagcggttcg aggagttgaa catcctatgg
     1741 atcgaggaac cggccgaccc gttcgacgtc ggcgccttga agaggatctc agaccgggtg
     1801 gacatcccga tcgcagtcgg tgagcggatc tatacccggc acggctttcg caagattttc
     1861 gagccgcacg cggccgacat cgtgcagccg gacgccggaa atacaggcgg aatcatggag
     1921 acgaa
//
LOCUS       DATLJT010001441         8396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1044917,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001441 DATLJT010000000
VERSION     DATLJT010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044917"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VM910_33090"
     CDS             complement(<1..1459)
                     /locus_tag="VM910_33090"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67377.1"
                     /translation="MAPSTADERERPPEIDVAFDAVVVGAGFAGLYMLYRLRGLGLTA
                     RVYEAGGGVGGTWYWNRYPGARCDVESMQYSFSFSEELDQQWDWSEKYAPQPEILAYA
                     NHIADRFDLRQNIVFNTRVTAATFDETAKRWRVETDRGDRVVARFCIMAIGCLSATNH
                     PAFTDREAFRGPIYHTGEWPHEGVDFAGLRVGVIGTGSSAIQAIPIIAQQASALTVFQ
                     RTATYSVPAWNEKLSPEYRKSIKADYPALRAKARARPTGFYFPFNMQPALEASPKERE
                     RQYEEAWKRGGLPFLGAYGDLLFDKAANDTIADFARRKIRGIVKDPATADLLCPDNVF
                     GCKRLCVDTGYFETYNLPHVKLVDVSKTPIERFTTQGIEVNGTEYHLDAIVCATGFAA
                     MTGSFEKIRITGRQGLTLAEKWQAGPRAYLGVASAGFPNLFTITGPGSPSVLASMIQA
                     IEQHVDWMADCIAHMRDIGAATVEPVEKYEDEWIEH"
     gene            complement(1606..2487)
                     /locus_tag="VM910_33095"
     CDS             complement(1606..2487)
                     /locus_tag="VM910_33095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67378.1"
                     /translation="MAAAGGADSIPAIPEEDASGDIATLYADLRATLGVPFVNLIWRH
                     LATIQGGLAWAWTLVKPLYVSGELVSRAAETSALVRLPSLAPLPDFVWDGAAILRADR
                     QVIVKLIDSYNHANGVNFLALLVAISALREGLPMPVGTERVAPKATGHGAEADALPAL
                     PRLSELSPTVLALARRLDEFGRLGPSDAIASLYRHLAHWPAFLSIAYVALEPLHRSGE
                     LARAQESVIAFGRRQSVELANLVDCDLPAITPESRSAVLSALDEFTRLMIGRMIVTGK
                     AMRSLIPPDGEAQYERH"
     gene            complement(2489..3679)
                     /locus_tag="VM910_33100"
     CDS             complement(2489..3679)
                     /locus_tag="VM910_33100"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI67379.1"
                     /translation="MSALKGIRVLDLGTFIAGPHCATILGEFGAEVIKVEPPKTGDSL
                     RRLGTNTECGDTLVWLSEARNKKCVTLDLRSKRGRELLRKLAAKCDVIVENFRPGTLE
                     KWGLSYEELKKLNRAAILVRISAYGQDGPMRSQPGFARIAHAFSGLTYLAGMPGGVPV
                     VPGSTSLADYMSGMYGAIGALVALRARETTGEGQCVDLALYESVFRVLDEIAPAYQKF
                     GYVRERMGADTVNVCPHSHYQTADGKWIAIACTSDQMFVRLAETMGQPELASAERYGP
                     KDLRLAARDEVNAIVARWVASLDLETALARCARGGVPASLIYSIADIFEDPQYSARGN
                     IKMTDSRAGAIAVPDVVPRLSATPGEIRWLGEGLGAQNEDVFENLLGLEGNEVQRLRE
                     DGVI"
     gene            complement(3676..4635)
                     /locus_tag="VM910_33105"
     CDS             complement(3676..4635)
                     /locus_tag="VM910_33105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HVI67380.1"
                     /translation="MISKSTFRSLLAVPATSPRFLEKAAQGPADAIFIDLEDAVIASL
                     KPKARREAIAALNQLDWGSRYVAVRVNGFGTSWCCRDILDVVEACPRLDYILLPKCEE
                     PADVHAVEVMIQSAEQAAPRQREVGIMGLIETPRGVANVEAIARSGGRLRALVFGGGD
                     YQLDLGSLQRAVGAPSADYFVLTDDDGRGARERHWNDLWHFATARIANACRAFGIIPI
                     DGPFSGIRDVEGLRAATKRASVLGFECKMAIHPSQIEIIHEVLTPTADQIAWAKEILE
                     AMAAAEREGRGAVKDKNGEMIDLMHIKVANKLLERAARIGCKS"
     gene            complement(4632..5591)
                     /locus_tag="VM910_33110"
     CDS             complement(4632..5591)
                     /locus_tag="VM910_33110"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVI67381.1"
                     /translation="MALSLPRKVVVAEVGPRDGLQSFPRFIDTDTKVAMIDKLSDLGF
                     PVIEVSSFAHPNIVPHLRDAEEVFERIKRRPGTVYRALVPNARGAERGAKARVDEMLG
                     LITISATYTRKNQNMTIDQAIAQNLTSFKIADTSGIPFVMALGMAFWCAYEGQIPEEN
                     VIAVVQRFHDGGIRRFYLAGSLGMEDPSHVNRLFHRLAELFPSASFGFHIHNLSGMAT
                     ANILAALDAGAEWLEGAICGIGGGIAMPTRLGSVGNFPTEDLVAMLAEMGIETGIDPE
                     RTVTVSREIAAMLGIDPQSHRANGATRKAVAELATHSPNMRYS"
     gene            complement(5601..6308)
                     /locus_tag="VM910_33115"
     CDS             complement(5601..6308)
                     /locus_tag="VM910_33115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HVI67382.1"
                     /translation="MHEKLSPALAASAVDDAIRTRQSVRAFLPTPVERATVEELLALA
                     SRSASGSNIQPWRVRVVAGEVKERLTRAIFDAIARDGFEPYTREWNYYPVNWREPFLS
                     RRRKIGWDMYGLLGVGKGDFEGTQRARMQNFEFFGAPVGMIFTLDEDLEIGSWLDLGI
                     YLGALMIAARGRGLHTCPQAAFADFHRIIRPLLGIPEKEIIVCGMALGRIDPHAPVNA
                     LVTERAPPSDFATFEGL"
     gene            complement(6301..7065)
                     /locus_tag="VM910_33120"
     CDS             complement(6301..7065)
                     /locus_tag="VM910_33120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67383.1"
                     /translation="MTDEAAPLALKDVTSGYGDVAVIRRVSMTFPKGIITTIVGSNGA
                     GKSTAIKVAAGLLRAWKGSISVNGVDITREGPDRRVHRGIAYVPQGRIVVPEMTVRDN
                     LMIGAHILGNNRAAVDAATERVLQLFPVLKPRMKQQADTMSGGEQQMLAIGRSLMTSP
                     EIIMLDEPSLGLSPKFVDIVFEKLLSLRNEGMTVVMVEQKASHALSVSDRGYVMHLGQ
                     VAIEGAAQELLSNDDVKRLFLGEIPESMKALSFFDA"
     gene            complement(7073..8242)
                     /locus_tag="VM910_33125"
     CDS             complement(7073..8242)
                     /locus_tag="VM910_33125"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI67384.1"
                     /translation="MIRRLLLAATAAALSLAAPAYAADPVRIGVALSQTGNLADSAAP
                     YFKGLDLWREQANARGGLAGHPIEFVVYDDRSDPATAARLYEKLITDDKVNLVISSLG
                     SATAATGSAVAEKHKVIMINGGGAAEAIQQRGFKYVFQTAARISSYADGIVPMIEKYK
                     IKSMALVSRDYAAARDISKTIKEQIKEKDVKVVMDEYFPAGTADFSSQIAKGQQLQPD
                     LWIGLLYPSEAIETVRQFHSMNYMPKFFIANGVSQDDFITSAGKDAEYALGMSLYEPS
                     LPSEGNKEFVKTYHDKYNSDPGYYAAFGFVAGTVLEAAVKKAGSIDTEKVREVLTTLK
                     LGTVMGKHEVDPTTYMQIGVRGLVVQVQNGKREVVWPEEYRTAEPKLPIPAWDKR"
BASE COUNT         1580 a         2550 c         2583 g         1683 t
ORIGIN      
        1 cgtgctcgat ccattcgtcc tcgtatttct cgacgggctc gacggtggcc gcaccgatat
       61 cccgcatatg agcgatgcag tccgccatcc aatcgacgtg ctgttcgatg gcctggatca
      121 tgctcgccag tacggacggg ctgcctggac cggtgattgt aaaaaggttg ggaaagcctg
      181 ccgacgccac gcccaaatag gcccgaggtc ccgcttgcca tttctcagcc agcgttaacc
      241 cttgacgtcc tgtgatccga attttctcga acgatcccgt catcgctgcg aagccggtcg
      301 cgcaaacgat tgcgtccaga tggtattcgg taccgttcac ctcgatgcct tgcgtggtga
      361 aacgttcgat cggcgtcttc gacacgtcga ccagcttcac atggggaagg ttgtaggttt
      421 cgaagtatcc ggtatcgacg cacaggcgct tgcagccgaa gacattgtcc gggcagagca
      481 gatcggctgt tgccggatcc ttgacgatgc ctcgaatttt gcgccgcgca aagtcggcga
      541 tcgtgtcgtt ggcagctttg tcaaacagaa ggtcgccata agctccaagg aagggcaggc
      601 caccgcgttt ccaggcctct tcatattgtc gctcgcgctc ttttgggctc gcctccaaag
      661 caggctgcat attgaacgga aaatagaagc cggttggccg cgcacgcgct ttggcacgca
      721 gtgccgggta gtcggccttg atggactttc gatactccgg cgacagcttc tcgttccaag
      781 ctggcacgga ataggtcgcc gtgcgctgga atacggtgag ggctgaggct tgctgcgcga
      841 taattggaat agcttgaatt gccgaggagc ctgtaccaat gacgccaacg cgaagcccgg
      901 cgaagtctac cccttcatgg ggccattcgc cggtgtgata gattgggccg cggaaagcct
      961 ctcgatccgt aaaggccgga tggttcgtcg ccgaaagaca gccgatcgcc atgatgcaga
     1021 atctggcgac gacgcgatcc ccacggtcgg tttcgacacg ccagcgcttg gcagtttcgt
     1081 caaaggtagc cgccgttacg cgggtattga acacgatgtt ctgacggaga tcgaaccggt
     1141 cagcaatgtg gttggcataa gcgaggatct cgggttgcgg cgcgtatttt tcagaccagt
     1201 cccattgttg gtccaactcc tccgagaagg agaaggaata ttgcatgctc tcgacatcac
     1261 aacgggcgcc cgggtaccga ttccagtacc aagttccgcc cacgccgccg ccggcttcat
     1321 agacccgcgc cgtaaggcca aggccgcgaa ggcgatagag catgtaaagg ccggcgaaac
     1381 ccgcgcccac gacaacagcg tcgaacgcaa catcaatttc cggcggacgc tcgcgttcat
     1441 ctgcggtgct gggagccatg ggtgttgtct tccaaactaa tgacgtcgcc tattttcaat
     1501 gtgggccacg ggttggttcg gcgcgaccgg gcgaacgcag cagcgatttg ccgattagat
     1561 cagccgaaag cagatcggta gtcaattgcg ttataaatcg gtgtttcagt gtctttcata
     1621 ctgcgcttca ccgtcaggcg gaataaggga acgcatggcc ttacccgtga cgatcatgcg
     1681 cccgatcatc aggcgggtga attcatcgag ggcgcttaga acggcgcttc tgctctccgg
     1741 cgtaatagcg ggaaggtcgc aatcgacgag gttcgcaagt tcgacgcttt gtcggcggcc
     1801 aaaagcaatt acgctttctt gcgcgcgcgc aagttcgccg ctgcgatgga gaggctcaag
     1861 cgctacgtag gcgatggaca ggaatgctgg ccagtgcgcg aggtggcggt acaggcttgc
     1921 gatggcgtcg ctcggtccga gccgaccgaa ctcgtcgagc cggcgcgcca aagccagcac
     1981 ggtgggcgac aattcagaaa gcctgggcag tgcgggcagc gcatccgctt cggcaccatg
     2041 cccggtggcc ttcggtgcga cgcgttcggt tcccacaggc atcggcaggc cctcgcgaag
     2101 cgccgatatg gcgacgagga gcgcgaggaa gttgactccg ttcgcgtgat tgtagctgtc
     2161 gatcaacttc acgatcacct gccggtcagc gcgaaggatc gcggcgccgt cccagacaaa
     2221 atccggcaat ggagcgagcg aggggagacg gacaagagcg gatgtctcgg cggcacgtga
     2281 aacaagttca ccgctcacgt aaagcggctt caccagcgtc caggcccatg caaggccacc
     2341 ctggatggtt gcgagatgac gccagatcag gttgacgaag ggaacgccga gcgttgctcg
     2401 caaatccgcg tagagggtcg cgatatcgcc cgacgcatcc tcttcgggga tcgctggaat
     2461 cgaatcggcg ccgcctgctg ccgccatcct agatcactcc gtcctcgcgc aatctctgaa
     2521 cctcgttgcc ctcgagcccg agcagattct caaatacatc ctcgttttgc gcaccaagcc
     2581 cttcgccgag ccatcgaatc tcacccgggg ttgcggacag gcgaggcacg acgtcgggaa
     2641 ccgcgatggc gcctgcgcgc gagtccgtca tttttatatt gccgcgtgcg ctgtattgcg
     2701 ggtcttcgaa aatgtccgcg atgctgtaga tcaggctcgc cggcacgcca cccctagcgc
     2761 aacgcgcaag tgccgtttcg agatcgagcg aagcaaccca cctggcgacg attgcattga
     2821 cctcgtcgcg cgcggcaagc cgcaagtcct tcgggccata tcgttcagcc gaagcaagtt
     2881 cgggctgccc catcgtttct gcaagccgaa cgaacatctg gtcgctggtg caggcgatcg
     2941 cgatccactt gccgtccgcg gtttgatagt ggctgtgcgg gcagacattg acggtgtccg
     3001 cgcccatacg ctcgcgcaca tagccgaatt tctgataagc gggtgcaatc tcgtcgagca
     3061 cgcgaaatac ggactcgtaa agcgcgaggt cgacgcactg gccttcgccg gtcgtctcgc
     3121 gtgctcgcag cgcaaccaga gcgccgatcg ctccatacat tccggacatg tagtcggcaa
     3181 gcgacgtcga gccgggcacg accggcactc cgccgggcat tcccgcaaga taggtgagac
     3241 cggaaaatgc atgcgcgatg cgggcaaagc cgggctgcga ccgcatcggt ccgtcctgtc
     3301 cataggcgga tatccggacc aggatagcgg cgcgattgag cttcttcagc tcctcatagg
     3361 agaggcccca cttctccagt gtgccgggac ggaaattttc gacgatgacg tcgcatttcg
     3421 cagccagctt gcgcagcaac tcgcggccgc gtttgcttcg caaatcgagg gtgacgcatt
     3481 tcttgttacg ggcttccgac agccagacca gcgtgtcgcc acactcggta ttggtgccga
     3541 gccgcctgag ggaatcgccg gtcttcggcg gctcgacctt gatcacttcg gcgccgaatt
     3601 ccccgagtat ggtggcgcaa tgcggtcctg cgatgaaggt gccgaggtcg aggacgcgga
     3661 ttcccttcag cgcactcatg atttgcaccc gatccgcgcg gcgcgttcga gcagcttgtt
     3721 ggcgaccttg atgtgcatga gatcgatcat ctcgccgttc ttgtccttga ccgcgcctcg
     3781 cccttcgcgc tcggccgcgg ccatggcctc caggatctcc ttggcccagg cgatctgatc
     3841 cgccgtcggc gtcagcacct cgtgaatgat ctcgatctgg gatgggtgga tcgccatctt
     3901 gcattcgaag ccgagcacgc tggcgcgctt tgttgctgca cgcagtccct ccacatcgcg
     3961 gatgccggag aatggcccat cgatcgggat aatgccaaaa gcgcggcatg cattggcgat
     4021 ccgcgccgtg gcaaaatgcc agagatcgtt ccagtgtcgc tcccgcgcgc cccgaccgtc
     4081 gtcgtctgtc agcacgaaat agtccgccga gggcgcgccg accgcgcgct gaagactgcc
     4141 gaggtcgagc tggtagtcgc cgccgccgaa caccagggct cgcagccggc cgcccgaccg
     4201 tgcaatcgcc tcgacattgg caacgccccg tggtgtctcg ataagtccca tgatgccgac
     4261 ctcgcgctgg cgtggcgcag cctgctcggc ggattggatc atgacttcca cggcatgcac
     4321 gtcggcgggc tcttcgcatt tcggcaacag gatgtaatcg agccgagggc aggcctcgac
     4381 gacgtcaagg atatcccggc agcaccagga tgtgccgaat ccgttgacgc gaacggccac
     4441 atagcggctg ccccaatcaa gctggttcag ggcggcgatg gcttcccgac gcgccttcgg
     4501 cttcaaggac gcgatgaccg cgtcctcgag gtcgatgaag atggcgtcgg cgggaccttg
     4561 cgcggccttc tccaggaagc gcggcgaggt ggcgggaacg gcaagcaatg accgaaatgt
     4621 cgatttggat atcatgaata tctcatgttt gggctatggg tggcgagctc tgcgaccgcc
     4681 ttgcgtgttg cgccgttggc gcggtgactt tgcggatcga taccaagcat ggcggcgatc
     4741 tcgcgcgaca cagtgaccgt ccgttccggg tcgatcccgg tctcgatgcc catctcggca
     4801 agcatcgcca ccaggtcttc ggtcggaaaa ttgccgaccg agccaagcct tgttggcatg
     4861 gcgatgccgc cgccgatgcc gcaaatcgcg ccttccagcc actctgcgcc ggcatcgagc
     4921 gccgcaagga tgttggcggt cgccatcccc gacaggttgt ggatgtggaa cccgaaagat
     4981 gcgcttggga agagttcggc aagccgatgg aacaggcgat tgacgtgcga ggggtcttcc
     5041 atgccgagcg agcccgccag atagaagcgg cgaatgccgc cgtcatgaaa tcgctgcacg
     5101 acggcgatga cgttttcttc cggaatttgt ccctcatagg cgcaccagaa ggccatgccc
     5161 agcgccatca cgaagggaat gccgctagtg tccgcgattt tgaaggatgt cagattctgc
     5221 gcgatcgctt ggtcgatcgt catgttctgg ttcttgcgcg tgtaagtcgc gctgatcgtg
     5281 atcagcccga gcatttcgtc gacccgtgct ttcgcgcccc gctcggcgcc tcgtgcattc
     5341 ggcacgagcg ctcgatagac cgtcccgggc ctgcgtttga tccgctcgaa gacctcttcg
     5401 gcatcgcgca aatgcggaac gatgtttgga tgagcgaagc tcgacacttc gatgactgga
     5461 aacccgagat cggatagctt gtcgatcatc gcgaccttgg tgtccgtgtc gatgaagcgc
     5521 ggaaagctct gcagcccgtc gcgcgggccc acctcggcaa cgacaacttt cctgggaagg
     5581 ctgagtgcca tcaggtttcc tcacaaccct tcgaacgtcg cgaaatcgct gggtggcgcg
     5641 cgctcggtta ccagcgcgtt gaccggcgca tgtggatcga tacggccgag ggccatgccg
     5701 caaacgatga tttccttttc ggggatacca agcagcggtc ggatgatcct gtggaagtcg
     5761 gcaaacgcgg cctgcgggca ggtgtgaagt ccgcgtcctc gcgcggcaat catcagcgct
     5821 ccaaggtaga ttccgagatc gagccagctt ccaatctcaa ggtcttcgtc gagtgtgaag
     5881 atcattccga ccggagcgcc gaaaaactcg aaattttgca tccgagcgcg ttgcgtgcct
     5941 tcgaaatcac ccttgccaac gccaagcagg ccatacatgt cccagccgat cttgcggcgc
     6001 cgggacagaa acggctcgcg ccagttgacg ggataatagt tccattcgcg cgtgtagggc
     6061 tcgaagccat cccgcgcgat ggcgtcgaag atcgcccgcg tcagccgctc cttgacttct
     6121 cccgcgacga cgcggacgcg ccacggctgg atgttgctgc cgcttgcgct tcggcttgca
     6181 agcgcgagca gttcctcgac cgtggcccgc tcgacggggg tcggcaggaa ggcccgtacc
     6241 gattgacggg tcctgattgc gtcatcgacg gcggaagccg caagggctgg cgaaagtttc
     6301 tcatgcatcg aagaagctca gcgctttcat gctttcggga atctctccga ggaacagccg
     6361 tttgacatcg tcattggaaa gaagttcttg cgctgcgccc tcgattgcca cctggccgag
     6421 atgcatcacg tagccgcggt cggaaacgga gagtgcatgc gaggccttct gttcgaccat
     6481 gaccaccgtc atgccctcgt tgcgcagcga cagcagtttc tcaaacacaa tgtccacgaa
     6541 tttgggcgac aagccaagcg acggctcgtc cagcatgatg atctccgggc tcgtcatcaa
     6601 cgagcggccg atcgcgagca tctgctgttc gccgccgctc atcgtgtcgg cctgctgctt
     6661 catgcgcggc ttaagcacgg gaaacagttg cagcacgcgc tcggtcgccg cgtcgaccgc
     6721 ggcacggttg ttgccgagaa tgtgcgcacc gatcatgagg ttgtcgcgta ccgtcatttc
     6781 ggggacgacg atgcggcctt gcgggacata ggcgatcccc cgatgaaccc gccggtcagg
     6841 tccttcccgt gtgatatcca ctccgttcac ggagatgctg cctttccagg cgcgcagcag
     6901 gcccgccgcg actttgatcg cggtcgactt gccggcgccg ttggagccga cgatggtcgt
     6961 aatgataccc ttggggaagg tcatgctgac gcggcggatc accgcgacgt cgccgtaacc
     7021 cgacgtcacg tccttcagcg ccagcggtgc cgcttcgtcc gtcattctgg tctcagcgct
     7081 tgtcccaggc cgggatcggc aatttcggct cggcggtcct gtactcctcc ggccagacga
     7141 cttcgcgctt gccgttctgg acctgcacca ccagcccgcg cacgccgatc tgcatatagg
     7201 tcgtcggatc gacctcgtgc ttgcccatga cggttccaag cttcagcgtg gtgaggacct
     7261 cgcggacctt ctcggtatcg atcgagccgg ccttcttgac cgcggcttcc agcaccgtgc
     7321 ctgcgacgaa gccgaaggca gcgtagtagc cgggatcgga gttatatttg tcgtgatagg
     7381 tcttcacaaa ttccttgttg ccttcgctcg gcaacgacgg ctcgtacaag gacatgccaa
     7441 gcgcatattc ggcgtccttg cccgccgacg taatgaaatc gtcctgtgat acgccattgg
     7501 caatgaagaa ttttggcatg tagttcatcg agtgaaactg gcggacggtc tcgatggctt
     7561 cgctgggata gagcaggccg atccagaggt cgggctgcaa ttgctgaccc ttggcgatct
     7621 gcgacgagaa gtccgcggtg ccggccggga aatattcgtc catcacgacc ttgacgtcct
     7681 tctccttgat ctgctccttg attgtcttgc tgatgtcgcg ggcggcggca tagtcacgag
     7741 agaccagggc catcgacttg atcttgtatt tctcgatcat cggtacgatg ccgtccgcat
     7801 aggaggagat gcgcgccgcg gtctggaaga catatttgaa gccgcgctgc tggatggcct
     7861 ccgccgcgcc gccgccattg atcatgatga ccttgtgctt ttcggcgacg gccgatccgg
     7921 ttgcggcggt cgccgaaccg agcgaggata ttacgaggtt gaccttgtcg tcggtgatca
     7981 gcttttcata aagccgcgcg gccgtcgcgg ggtcggagcg gtcgtcatag acgacgaact
     8041 cgatcgggtg accggccaac ccgccacggg cgttggcctg ctcgcgccag aggtcgagcc
     8101 ccttgaaata gggagctgcg gagtccgcga gattgccggt ctgggagagc gccacgccga
     8161 tccgaacggg atcggcggcg tacgcgggag ccgccagcga aagtgcggcc gccgttgcag
     8221 ccaggagaag tcgtcttatc atgttgcacc ttcctatgat gacaaatcag aaataggcct
     8281 gcagcacttt ggcgtcgttg cgcaccgtct gcgtgtcgcc gcgcgcgatc agcttgcctt
     8341 cggcgagcac atagacaaga tcgcactccc tgagcacgat ccgcatttcg tgatcg
//
LOCUS       DATLJT010001442         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3343362,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001442 DATLJT010000000
VERSION     DATLJT010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343362"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1238..1591
                     /locus_tag="VM910_33130"
     CDS             1238..1591
                     /locus_tag="VM910_33130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67385.1"
                     /translation="MPGTRRTPINRQHKPPVSELAIRLFMKMQRCRNCDRWWDLHTEL
                     RREVGAKLWERPCIEDPHAGNPEKPGTYNYERWKPDEKARARWRALEQGARELRRRER
                     EVRRAAKAIPEHEPA"
     gene            1588..1788
                     /locus_tag="VM910_33135"
     CDS             1588..1788
                     /locus_tag="VM910_33135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67386.1"
                     /translation="MSPIALTDTQLRELMQAANMVPWDPRDAFLERVAAEIRGREILG
                     DGTVHRVACEVARTIAWDAESP"
     gene            complement(1944..2264)
                     /locus_tag="VM910_33140"
     CDS             complement(1944..2264)
                     /locus_tag="VM910_33140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67387.1"
                     /translation="MSDHSRIIPFPQDGRPPPPEHLSPEEAETWKATVGARKAGFFGP
                     EIFPLLEAYCATAMICDRIAAQLRELDEIDHEAYETYDRMTRSLADLAKALCLLPEGE
                     RTDA"
BASE COUNT          602 a          954 c         1039 g          652 t
ORIGIN      
        1 gccagctcct cgccgccggc aaagtccgct gtgacaagca gaagctcaac ggccccggcc
       61 gcgcgcgcga gctttggttt gcagtgtgaa agaagcgcaa ggatcatggc ctggaaagac
      121 accgagcgcg agcgttggtc acccgagctc gcttgctgcg tccgccggat cacctacgac
      181 tttgagcggg gacgggccgg ctcgacatgc cgcctgtgac ctgctgcgac atggaaggct
      241 gcatcaacct cttccgcgcg atcgacctcg gggtgcgccg gatcgaaacc tacgccggcg
      301 gtcagcgcga tacgttttat tggcgcgacg accgagatcg gtggtgcgcg tcgaaccact
      361 gcggacgtcc acttcggctg ggcgccaatt cgcctacagc aatgacgcgt gaccggcagc
      421 tcgcgagact acaactctaa attttgtcgt ttaattctta attcgtacgg cctttgcgtg
      481 ggaaatgcgc tcgtttgcga tcctagcccg tttaatctaa attctgcaat aatatcaata
      541 ctcaagttct tgactctcaa ttcgtcggag tgcggtttta aagggcaccg ccgacgaact
      601 aattaattcg gcatctgccg tcggtggagc ctgttcgtcg tcaggttgta ttgagcgggg
      661 cgtcaaggag actccccgcg cgcggccatc gggtcccgtg ccggccaacg ggtcctccga
      721 ggctggccaa aaatttagtt cttgggggct taggggtggt cattggaccg ctgcaactac
      781 cccagtgcgc cctccaagga atggtcgacc ggttgggctg ttgtgcggtt cgcgacatcg
      841 agttcgttag cctcgccata ctgcggcgcc cgcgcagcag gtcaaaaccc aaggtgccca
      901 accgcgcggt ggaaaaggca ttccacaccc acattctcgc tcgtctacgg ctgaatcctt
      961 ggctgtaggg ctacgcccgc gaaagctgct gctccgagct cgatcggaga gggtgagggc
     1021 gcccaaaact tctcaagcaa ttccgaaaag ggaggggggc gcgaaaattt tcaaacgatg
     1081 acctggtttt cgagcgcagt cgcggtcggc ctgaatcgcg aataatccgg tcttggacct
     1141 gaagttttgt tgcgaaaatc gccaaatcgc ccccgggctt gccggtgaag ccgcatgggg
     1201 ggcagccgcg cactattggc tggaccggag aacgctgatg cccggcaccc gccgcacgcc
     1261 gatcaaccgc cagcacaagc caccggttag tgagctggcg atccggctat ttatgaagat
     1321 gcagcgctgt cggaactgtg atcggtggtg ggatttacac accgaactcc gtcgcgaggt
     1381 gggcgccaaa ctgtgggaac ggccatgcat cgaggatccg catgccggca atcccgaaaa
     1441 gcccggcacg tacaactacg agcgctggaa gccggacgag aaggcacggg cgcgatggcg
     1501 cgcgctcgag caaggtgccc gtgagctgcg gcgccgtgag cgcgaggtgc gacgggctgc
     1561 taaggccatt cccgagcacg agcccgcatg agcccgattg ccctcaccga cacgcagtta
     1621 cgtgagctca tgcaggcggc gaacatggtg ccgtgggatc cccgtgacgc gttccttgag
     1681 cgggtggcgg ccgagatccg cggccgggag atcctcggcg acgggaccgt ccaccgcgtg
     1741 gcttgcgagg ttgcccgcac gatcgcatgg gacgccgaga gcccgtgatg ttctgcgacc
     1801 gcaacgtaca cgccagagag tggccgccag cgcgcctaac ccgtcgccgc cgtccgcccg
     1861 gcgtccatcg cgtagatccg gtggtaccat ccgcgccgct tgcattagct cgtggagctg
     1921 ggcgtcggta aaccgaatca gcgtcatgcg tccgtccgtt cgccctcggg tagcaggcac
     1981 agcgccttcg cgaggtccgc gagcgatcgt gtcatccggt cgtacgtttc gtaagcctcg
     2041 tggtcgattt catcgagctc acgtaattgc gctgcgatgc ggtcgcaaat catcgcggtt
     2101 gcacagtatg cttcgagcag cgggaagatc tctggaccaa agaacccggc cttccttgcc
     2161 ccgacggtcg ccttccaggt ctcggcttct tcaggagaca gatgctccgg cgggggtggt
     2221 cgtccgtctt gggggaaggg aatgatcctc gagtggtcgg acatcgctat cgctcccaaa
     2281 gtctgggctt gcgaggggtg tcatcctcgt agggaaaatc ccatggtttc ggtcctatgg
     2341 tgcggtgcgg atcgcggccg tcggcgcggc tctccaaatt agatttgggc gtgagcctta
     2401 atgcgcgtgc gaatgccagc gcggtggagg ctgtcgagtt gagccgttgt gatagccggt
     2461 cgtaactcgg gagcccgacg ccgatacggt caagctccgt ttcgagcctc tcgcattcag
     2521 ccatggcgtg acagtagcgg gcgaggagcg cgtgcgtctc cttgctgaag tggcgcgctt
     2581 tcatacttga aactgcatct ctccagacgg cagcggcggc ctcgggcatt ccctgcggtg
     2641 ggtcggggcg ttcatgcgtc cacgggacag cggggctatt ggcaaggtgg ttgatttcgt
     2701 tggcggattt tcggggcacc agagccttcc tcgttgggtt atagaaaatc gcctgaaccc
     2761 cggccgaaat agcattctct gagccaaaaa tctatcaaaa agcgcataat tcagctggtt
     2821 tctcaatggc ttgcagtgtg gctttttgtc cggacctttt gttggaatag gtagtccgtt
     2881 gcaaagatcg agcgcagccg cgcccggagg gtggcctggt cttgaaaaaa atcgacgccc
     2941 cccgccatcg aaaagcgccg aaggtctgcc gcgttggaat tttggcgcgg gcgagcgtcg
     3001 gatatcgatt cgcacatcgc ttgccctgcg caattccttc gttgaatggc tgacggcggg
     3061 actggatagt gcgcatgatt ccgcagccgg aaccgtcgac ccaggaagtg ctggcgggcc
     3121 tcgtcgagcg agtaacctac cacaacgccg agaatggttt ttgcgttctg cgcgccaggg
     3181 cgcgcgggca tcgtgacgtc gtgaccgtgg ttggctatgc cgcaaccatt tccgccggcg
     3241 agtggat
//
LOCUS       DATLJT010001443         4201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1278617,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001443 DATLJT010000000
VERSION     DATLJT010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278617"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VM910_33145"
     CDS             complement(<1..240)
                     /locus_tag="VM910_33145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HVI67388.1"
                     /translation="MLKLPGHTRYAYSPIVERADYSWPGGKRLAFYVALNIEHFAFGA
                     GLGMDPVRSGQQTTRNFAWRDYGNRIGNWRLFEILD"
     gene            complement(616..2325)
                     /locus_tag="VM910_33150"
     CDS             complement(616..2325)
                     /locus_tag="VM910_33150"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HVI67389.1"
                     /translation="MKTLEEASSTSSSSPSDRRHIRPDVTLRAVRQWARERRIGRARL
                     THAYQAAFLVAVSRLLQAIFRERHAPHARLWQSDARQWFLDLGREPMLRAPVSGPLPF
                     RRLELTGFPWKIVAGRRRLLRSTRAFLNALRPCVGPSQLAQHFDRLIADFDNSFANLV
                     MNHLIGERLDAGAQAIEPVYEGHHYYPFPALRLGPSLRQVVECSNLCREPIDLTLVAA
                     RPCLFDSTVYADHRTCFRAWAGIPLPRNADVVIPLHPWQLELSPVVRELLKRRWIAVL
                     DRRIKAVPLASQRTCRILRSGFDVKLPIAVTLTGEDRLLYPLNRANATAFSSLARILL
                     RASGESTLDFQYDVASIAHAESLIGTHLAVIVRAPVRSRGAEIVVPALNLWCGPRWAR
                     TMLDLRRREHAHAFFRIYCRVLMRGMVDFYARGMAFEPHLQNVYVALRDGMPSRMILR
                     DLDSTILDPLRIRPVARANGVRFALGTWKHMPDFATGGRRLAHAMMYGHLGQVMSYLA
                     RAAQADLAKLSAAVEETWDELIAQAPSAACRGPVRDLRKQADTVGAVLWRRITRADHT
                     AFR"
     gene            complement(2276..3040)
                     /locus_tag="VM910_33155"
     CDS             complement(2276..3040)
                     /locus_tag="VM910_33155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67390.1"
                     /translation="MNIAAGLTLEALRPHGVAAWPHYRDALVTACRKAPPPFGTKGYG
                     KIYRDVAKDPDWMANSLIQNAQGEGEGSGHLWDLAASTPDPRIAAQVKAHAIDESRHS
                     KAYVAMLDLAFPDLIDNNELHAQLASLSPGYSQRSPLEAHKGSAYASAITLDDLIQMN
                     IAEIRTLVHHILQRPMLLGYCAPERRHRLTLLHDRLRLDEVRHIAYTAALIEEFAQRG
                     EAGAVKRLMQERMSDFNAITNEDLGRGVFDFELEPL"
     gene            complement(3066..3296)
                     /locus_tag="VM910_33160"
     CDS             complement(3066..3296)
                     /locus_tag="VM910_33160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67391.1"
                     /translation="MAKRAKRGTYSHATQALAGARVPKQEFGQFHPDGTLKIDLKKLE
                     ALKKKLGKTTWRKVRFVALNAPFKRRSPISPA"
     gene            complement(3454..>4201)
                     /locus_tag="VM910_33165"
     CDS             complement(3454..>4201)
                     /locus_tag="VM910_33165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HVI67392.1"
                     /translation="ELGVFDEGRDHGEEAVRLAEALDHRFSLIWACLNLGRLEGLRGE
                     FIRAIMLLERAVALSTERNIAYLTPIALAALGHVYALSGRVEEGVSWLQQALAGYASA
                     GIGYLRSMSMVQLGEAHLLAGRVEEAWDFGTRAVVLAREREERGHQAWAHRLLGETVS
                     HRDRPDVAAAEAHYATSTALASELGMRPLVAHCRFGLGKLYGRAGDRRATEHLTTAMS
                     LFHETGMRFWFEKAEAEMQALEVAVAPRRG"
BASE COUNT          776 a         1362 c         1359 g          704 t
ORIGIN      
        1 gtccaggatt tcgaacaggc gccagttccc aatccggttg ccgtagtcgc gccaggcgaa
       61 attgcgcgtg gtctgctggc cgctgcgcac cggatccatc cccagcccgg cgccgaaggc
      121 gaaatgctcg atgttgagcg cgacatagaa ggcgagccgc ttgcctcccg gccaactgta
      181 gtccgcccgc tcgacgatcg gcgaataggc atatcgggta tggccgggaa gtttcagcat
      241 cgggatgctc cgtgttggct cggtcggcta gcaacttgcc gatattttgg caaacgaacg
      301 cccgagcccc cgattacgaa gtttaacaaa acgaggtctc ggtgctcggg aatcctcact
      361 aaaattaaga cccccgccgc gataacctgt ccaccgtcga aagatgaatt gaataagcgt
      421 attttgggtt gcgcgcctgg atcgaccaga tcgtcagtca accagaacag caaggctcgc
      481 tcgcggttgc gatttcacaa ctcgatccga cataccctcg atagcctgat gcggcttgcg
      541 agttacgacc ggcccaccgg gtcgcaagcc aacgtggccg tgcaaaccaa cgggaaactt
      601 gacctgtcgc cgtgatcagc ggaaggccgt gtggtccgcc ctcgtgatcc ggcgccatag
      661 gacagccccg acggtatccg cctgcttgcg caagtcgcgc acgggccccc ggcaggctgc
      721 cgacggcgct tgggcgatca gctcgtccca cgtctcttcc accgcagcac tcaacttggc
      781 cagatcggcc tgcgcggccc gggccagata ggacatcacc tggccgaggt gtccatacat
      841 catggcgtga gccagtcgcc gaccaccggt ggcaaagtcc ggcatgtgct tccacgttcc
      901 gagcgcgaac cgcaccccgt tcgcccgcgc tactgggcgg atccgaaggg gatcgaggat
      961 cgtgctgtcg aggtcgcgca ggatcatgcg tgacggcatg ccatcgcgca gcgcaacgta
     1021 cacgttctgc aggtgtggct cgaaggccat gccgcgcgca tagaagtcga ccatgccgcg
     1081 catgagcaca cggcagtaga tgcggaagaa cgcgtgtgcg tgctcccgac gacggagatc
     1141 caacatcgtg cgcgcccacc gggggccgca ccagaggttc agcgccggca ccacgatttc
     1201 cgcgccgcgc gagcgcaccg gcgcccggac gatcacggcg agatgcgtcc caatcaatga
     1261 ctcggcatgg gcgattgatg ccacgtcgta ctggaagtcc agagtactct ctccgctggc
     1321 ccgcaagaga atgcgggcga gcgacgagaa agcagtcgca ttcgcgcgat tgagaggata
     1381 gagcaaccgg tcttcacccg tcagtgtgac ggcgatcggc agcttgacgt cgaagccgga
     1441 tcgcagaatc cggcacgtcc gctgcgacgc gaggggaaca gccttgatgc gccggtcgag
     1501 gaccgcgatc catcgccgtt tcagcagctc acggacaacc ggcgagagct cgagttgcca
     1561 agggtgcagc gggatcacga cgtcggcgtt tcgaggaagg ggaatgccgg cccaggcgcg
     1621 aaagcacgtc cgatgatccg cgtacacggt ggaatcgaag aggcacgggc gagcggcaac
     1681 caacgtcaaa tcgatcggct ctcggcagag attcgagcac tcaaccacct gccggagcga
     1741 cggcccaagc cggagcgccg ggaacggata gtaatggtgg ccctcgtaca cgggctcgat
     1801 ggcctgcgcg ccagcatcga gccgctcccc gatgagatgg ttcatcacca gattggcgaa
     1861 gctgttgtcg aaatccgcga tgagccgatc gaagtgctgg gcgagttgcg agggccccac
     1921 gcaggggcga agggcgttca ggaaggcccg ggtgctgcgt agcaggcgcc gtctgcccgc
     1981 gacgatcttc cacggaaatc cggtcagctc gagccgtcga aagggcagcg gaccactcac
     2041 cggcgcccgc agcatcggct cgcggccgag atcaagaaac cactggcggg cgtcgctctg
     2101 ccacaaccgc gcgtgcggcg cgtggcgttc ccggaagatg gcttggagca gccgcgacac
     2161 cgcgacgaga aacgcggcct ggtaggcatg ggtgagccgc gcccggccaa tccggcgctc
     2221 gcgggcccac tgtcgcacag cccggagcgt cacgtcaggg cggatgtggc ggcgatcaga
     2281 ggggctcgag ctcgaagtcg aagacgcctc ttccaaggtc ttcattcgtg attgcgttga
     2341 agtcgctcat ccgctcttgc atgagccgct tcaccgcccc ggcctcacca cgctgcgcaa
     2401 actcctcgat cagtgcggcg gtgtacgcga tgtggcggac ctcgtcgagc cgcagcctgt
     2461 cgtgcaggag cgtcaagcga tgccgccgct cgggggcgca gtatccgagc agcatcggcc
     2521 gctgaaggat gtgatgcacg agcgtccgga tctccgcgat gttcatctgg atgagatcgt
     2581 ccagagtgat cgcgcttgcg tacgcagagc ccttatgggc ttcgagcggg cttcgctggc
     2641 tatatccggg ggacagcgaa gcgagctggg catggagctc gttattatcg atgaggtccg
     2701 ggaaagcaag atccagcatc gcgacatacg ctttgctgtg tcgagactcg tcgatcgcgt
     2761 gcgccttgac ctgggcggct atgcggggat ccggggtact ggccgccaag tcccacagat
     2821 gcccggagcc ttcgccttcg ccctgggcat tctggatgag cgagttcgcc atccagtccg
     2881 ggtccttggc cacgtcgcgg tagattttgc cgtagccttt ggtcccgaac ggcggcggcg
     2941 ccttccggca ggcggtgacc agcgcgtcgc gatagtgagg ccacgccgcc acgccgtgcg
     3001 ggcgaagggc ttccagggtc agcccggcgg cgatgttcac aaccgcgccc cgatcgcgcg
     3061 gacgtctagg ccggggaaat tggcgatcgc ctcttaaacg gcgcattgag cgccacgaag
     3121 cggaccttac gccaagtcgt ttttcccaac ttctttttca atgcctcgag ctttttgagg
     3181 tcgatcttga gtgtgccgtc cgggtgaaac tgaccgaact cctgcttagg gaccctcgcg
     3241 cctgccagag cctgggtggc atgactgtac gtgcccctct ttgctcgttt cgccatcggt
     3301 gaatccctcc tgtcgggcgc gggcctatcg accatcgcgc gcggcattta tcaggcgccg
     3361 cgcgcatcga ccgaggctat ggtggcgaca ccgcaatgtc aaccgtgggt agtgtctcag
     3421 cttgaacccg ccccacaata tcaaactaag gcactacccg cgccgggggg ccacggcgac
     3481 ctccagcgcc tgcatctcgg cctcggcctt ctcgaaccag aaccgcatgc ccgtctcgtg
     3541 gaacaggctc atcgccgtcg tgagatgttc cgtcgcccgc cgatcgcccg ctcgcccgta
     3601 aagcttgccg aggccgaagc ggcagtgcgc gacgagcggg cgcatgccga gctccgaggc
     3661 caaagccgtg gaggtggcgt agtgggcttc ggccgccgcc acgtccgggc ggtcgcgatg
     3721 tgacaccgtc tctccgagga ggcggtgggc ccaagcctgg tgaccgcgtt cctcgcgctc
     3781 gcgggcgagc accacggcac gggtcccaaa gtcccacgcc tcctccaccc gacctgcgag
     3841 gaggtgcgcc tcgccgagct gcaccatgct catcgagcgc agatatccaa tcccggcgga
     3901 tgcatagccg gccaaggcct gctgcagcca ggagacgcct tcctcgacgc gtcccgacag
     3961 cgcgtagacg tgccccagag ccgccagcgc gatcggagtc aagtacgcga tgttccgctc
     4021 ggtggagaga gcgaccgcac gctcgagcag catgatcgca cggatgaatt ctccgcggag
     4081 gccttcgagg cgtccgaggt tcaagcaggc ccagatgagg ctgaagcggt gatcgagcgc
     4141 ttcggcgagt cggacagcct cctctccatg gtcgcgtccc tcgtcgaaca ctccgagctc
     4201 g
//
LOCUS       DATLJT010001444         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_358607,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001444 DATLJT010000000
VERSION     DATLJT010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358607"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VM910_33170"
     CDS             <1..607
                     /locus_tag="VM910_33170"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67393.1"
                     /translation="ERCYAFGQALARRVSAMGLNTVVLASGGMSHYPGTERYAAPDLA
                     WDQKTLERIAAGNLKSLIGYDSAELDAAGNVELRCWACAAGALGERKPDIVSLEPSWH
                     HNYASLGWFSPLEKPSELHYPSIKPELVELTVALHGLAHDEASRATFLADAAGYADRF
                     RLTSEQRAALIALDTSAIVAMGAHPLVPFLANMQVERQRRG"
     gene            699..1160
                     /locus_tag="VM910_33175"
     CDS             699..1160
                     /locus_tag="VM910_33175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI67394.1"
                     /translation="MEDQFRRPSLGSGVFYRDPWAAMDWLEKAFGFERSMVITDQDGR
                     LGHAEMRFGDGYIMPGSEWAEFIASPASVGGKNTQSIRVQLQDGIDAHCERARAAGAA
                     IVQEPADQFYGARTYRARDPEGHVWTFAQTVRHVSREEAEQASGLKIEGWD"
     gene            1157..1489
                     /locus_tag="VM910_33180"
     CDS             1157..1489
                     /locus_tag="VM910_33180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI67395.1"
                     /translation="MSQPADLDRILAALADPHRRRIVELLRDRPLRAGEIAEAVGLNP
                     PALSRHLRTLKANGLIAEAHPAFDARVRIYTLRPAPMTHLKAWLEQTEQLWAAQLSAF
                     KTYLERGP"
     gene            1486..1998
                     /locus_tag="VM910_33185"
     CDS             1486..1998
                     /locus_tag="VM910_33185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI67396.1"
                     /translation="MTSRVLVSLRVSATPERAFGVFVDDVGAWWRPNGLFRFTARSPG
                     VLAFEPRLGGRFTETSGTGEVFEIGRITVWEPGLRVAFTWRQASFTPEQVTQVEVRFE
                     PVGDETRVTVEHFGWDTVPQEHVARHGFPDAIFLRRHAEWWQSLLAAMARSVSMTGLR
                     RVPGSTVPQR"
     gene            2006..2326
                     /locus_tag="VM910_33190"
     CDS             2006..2326
                     /locus_tag="VM910_33190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HVI67397.1"
                     /translation="MSTSADLRRIALSLEGTTEAPHFDRTAFRARRIYVTLAADGKTA
                     NFMFTADQQEFKCMMASEAFAPVPNAWGKRGATTAILSKLTTAELEDALRTAWRRAVS
                     KKAW"
     gene            2528..>3061
                     /locus_tag="VM910_33195"
     CDS             2528..>3061
                     /locus_tag="VM910_33195"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67398.1"
                     /translation="MKIVNVSAIPMSAPVPEEKRHRTDLGTKVKSDATLIRVETDVGL
                     TGIGAALGTPPIVAAIVEHELAAECVGEDPMFSERIFEKMYNGSRSRPALERGITQAD
                     ESRRRGVIMEAIAGIDIAVWDVKAQALGIPLYQALGAARSSVRGYASGGWAPGEEAEA
                     ELTGYAAKGFTAAKMRVV"
BASE COUNT          548 a          958 c         1024 g          531 t
ORIGIN      
        1 ggagcgctgc tacgcctttg gtcaggccct ggcgcgcaga gtcagcgcga tggggctgaa
       61 tacagtcgtg ctggcgagcg gcggcatgtc gcattatcca gggactgaac gctatgcggc
      121 acccgacctt gcctgggacc aaaagacgct agaaaggatt gccgccggca acctcaaatc
      181 actgatcggt tatgactcgg ccgaactcga cgccgccggc aatgtcgaac ttcgctgctg
      241 ggcttgcgcc gcgggggcac tcggtgagcg caagcccgac atcgtctcgc tggagccgag
      301 ctggcaccac aattatgctt cgctcggctg gttcagtccg ctggaaaagc cgtccgaact
      361 gcactacccg tcgatcaagc ccgaactcgt cgaattgacc gtcgccctgc acggtctcgc
      421 ccatgacgag gcaagccgcg ccacttttct tgccgacgcc gccggctacg ccgatcgctt
      481 ccgcttgacg tcggagcagc gcgcggcgct gatcgcactc gacacaagcg ccatcgttgc
      541 gatgggcgcc catccgctgg tgccgtttct cgccaatatg caagtcgagc ggcaacgccg
      601 cggctgagag cctcgacatg gcttgccgct gaggaatctt cttgacatat atttcacggt
      661 ctggtgaaat attcgctcta gggagaagga ggtgtgccat ggaggatcag tttcgccgtc
      721 caagcctcgg ttcgggggtg ttctaccggg acccttgggc tgccatggac tggctggaaa
      781 aagcgtttgg cttcgagcgc agcatggtga tcacggacca ggacggccgg ctgggccatg
      841 ccgagatgcg gttcggcgac ggctacatca tgcccggttc cgaatgggcc gagtttatcg
      901 cgagcccggc ctcggtcggc ggcaagaaca cgcagtcgat ccgcgtccag cttcaggacg
      961 ggatcgacgc gcattgcgag cgcgcgaggg ctgccggtgc agcaatcgtc caggaaccgg
     1021 ccgaccagtt ctatggcgcc cgcacctatc gcgcgcgcga tccggaaggt catgtgtgga
     1081 ccttcgccca gaccgtccgt cacgtgtcac gcgaagaggc agagcaggcg agcgggctca
     1141 agatcgaagg ctgggattga gccagcctgc cgatctcgac cggatactgg cggcgctggc
     1201 cgacccgcat cgccgccgga tcgtcgaact gttgcgggat cggccgcttc gggcgggcga
     1261 gatcgccgag gccgtgggcc tcaatccgcc ggcgctgagc cggcacctga ggaccttgaa
     1321 ggcgaacggc ctgatcgcag aggcgcatcc ggcttttgac gcgcgtgtgc ggatctatac
     1381 cctgaggccg gcgcccatga cccatctcaa agcgtggctc gagcagaccg agcagctttg
     1441 ggctgcacaa ctcagcgcct tcaagacgta tctggagcgg ggaccatgac ctcgcgcgtt
     1501 cttgtttcgc tgcgtgtctc cgcgacgccg gaacgtgcgt tcggcgtgtt tgtcgacgac
     1561 gtcggcgcat ggtggcggcc gaacggcctc tttcgtttca cggcacggtc gcccggcgtg
     1621 ctcgccttcg aacccaggct gggtggccgg ttcaccgaga cgtccggtac cggcgaggtc
     1681 ttcgagattg gtcgcatcac ggtctgggag ccgggcctgc gcgtcgcctt cacttggcgg
     1741 caggcgagct tcaccccgga gcaggtcacc caggtcgagg ttcggttcga gccggtcggc
     1801 gacgagactc gagtgactgt cgagcacttc ggctgggaca cggttccgca ggaacacgtc
     1861 gcccgacatg gttttcccga tgccatcttc ttgcggcgtc acgcggaatg gtggcaatcg
     1921 ttgctcgcgg cgatggcgag atcggtgtcg atgacggggt tgcgccgagt accggggtca
     1981 acggtaccgc agcggtgagg gcccgatgtc caccagcgca gacctccgcc gcattgcgtt
     2041 gtctctggag gggacgaccg aagccccaca tttcgaccgc accgcctttc gggcgcggcg
     2101 catctacgtc accctcgccg cagacgggaa aacggcgaac ttcatgttca ctgcggatca
     2161 acaagagttc aaatgcatga tggcgtctga agcatttgcg ccagtgccca acgcctgggg
     2221 caagcgaggt gcgacgacgg ctatcctgtc gaagctgacg acagcggaac tggaagacgc
     2281 ccttcgcacg gcctggagac gggccgtgtc caagaaagcg tggtaagctc acaattatgc
     2341 ttcggctggc cggttcaggg cgccgccgag cggagcgacg aaacaatttc gaaggacgga
     2401 gcgcgacctg gcgcgagatt gcgtcccccg gaatgcccgc cctggcaggc ggccatccgg
     2461 gggtcgcgat gaagcctcct tttgaccaca ggcatttcgc gttcactaca ccagggagga
     2521 acgacccatg aaaatcgtca atgtgtccgc gattccgatg tcggcgcccg ttccagagga
     2581 aaagcgtcac cgcaccgacc tcggaaccaa ggtcaagagc gacgcaacgc tgatccgcgt
     2641 cgaaacagac gttgggctta ccggcatcgg ggcggccctc ggcacaccgc cgatcgtcgc
     2701 cgccatcgtc gagcatgagc tcgcggcgga atgcgtcggc gaggatccga tgttctccga
     2761 gcgcatcttc gaaaagatgt acaacggctc gcgcagtagg ccggcgctcg aacgcggcat
     2821 cacccaggct gatgaaagcc ggcgccgcgg tgtcatcatg gaagccattg ccggcatcga
     2881 catcgcggta tgggatgtca aggcgcaggc gctcggcatt ccgctctacc aggcgctggg
     2941 tgccgcgcgc agctcggtgc ggggctatgc gagcggcggc tgggcgccgg gcgaggaggc
     3001 cgaggcggaa ctgacgggct acgccgcgaa gggcttcacc gccgccaaga tgcgggtggt
     3061 g
//
LOCUS       DATLJT010001445         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_127592,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001445 DATLJT010000000
VERSION     DATLJT010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127592"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VM910_33200"
     CDS             <1..213
                     /locus_tag="VM910_33200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67399.1"
                     /translation="GHNRKITFSDFSYTEMVLEIEPGADAITLDSDPYVIIRPEGDWD
                     RPGVLDVSAGRVVTSPLRDATATARA"
     gene            227..784
                     /locus_tag="VM910_33205"
     CDS             227..784
                     /locus_tag="VM910_33205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVI67400.1"
                     /translation="MFEEDVPPILICADLQSEYLAAGRSHAISDPEAIMAGCLELMTL
                     WRENLWPVMHLKRIAQAAWFNPASNLTNWIAELKPKPGELAFEHPLPSAYSSARFAEY
                     MSNIRNIPCVMLGFSLDETILATVVDGFHRSHRYSVVSDAVACRCAGVGDAASYKAAV
                     VRVIGNFAGIRRSTDLIGASRRIPV"
     gene            781..978
                     /locus_tag="VM910_33210"
     CDS             781..978
                     /locus_tag="VM910_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67401.1"
                     /translation="MSTTPYRAGSAVEDDLRELAGDLATAIAKARALNLPTSTYLLRM
                     ALAEVSQTLSAEADENGGPAR"
     gene            complement(981..2129)
                     /gene="rnd"
                     /locus_tag="VM910_33215"
     CDS             complement(981..2129)
                     /gene="rnd"
                     /locus_tag="VM910_33215"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HVI67402.1"
                     /translation="MDLITTTSELAAACERLARHPVITVDTEFLRETTYYPLLCVVQM
                     ASPDEALVIDALADGIDLKPFFALMANEKVLKVFHAARQDIEIVWHRANIVPHPIFDT
                     QVAAMVLGYGDSIAYDQLVERITGHRPDKTHRFTDWSRRPLTKEQIHYAISDVTHLRD
                     VFIALDADLKKRSRSDWVSEEMEILTSPKTYDFHPERAWERLKTRVRKPKELAVLMEV
                     AAWREQEAQSRDVPRSRVLKDDAVGDIATHAPTTIERLANLRSLPKGFDRSKWGTDIV
                     AAVQRGLARDPATLPKLDKPRNNANGGAIVELLKVLLRMTSERHAVASKVIATVDDLE
                     QIAADDQADVAALHGWRRELFGEAALSLKHGKLALAIERGRVIRFDRR"
BASE COUNT          438 a          763 c          783 g          452 t
ORIGIN      
        1 ggccacaacc gcaagatcac gttttccgat ttctcctaca ccgagatggt gctggagatc
       61 gagccgggcg ccgacgcgat cacgctcgat agcgacccgt atgtgatcat ccgtcccgag
      121 ggcgactggg acaggcccgg cgtcctcgat gtttcagccg gccgtgtggt gacgtcgccg
      181 ctgcgcgacg cgacggcgac cgcgcgagca tgatccggtg cgcaagatgt ttgaagaaga
      241 cgtgccgccg atcctgattt gtgcggacct gcagtccgaa tatctcgctg ctggccgcag
      301 tcacgccata tcggatcccg aggcgatcat ggcgggctgc ctcgaactga tgacgctctg
      361 gcgcgagaat ctctggccgg tgatgcattt gaagcggatt gcgcaggctg cctggttcaa
      421 tccggcctcc aatctgacca actggatcgc cgaactgaag ccaaagccgg gcgagcttgc
      481 cttcgagcat ccgctgccat cggcctacag ctcggcgcgg tttgccgaat acatgtcgaa
      541 catcaggaat attccctgcg tgatgttggg cttttcgctg gacgaaacca ttctggcgac
      601 agtcgtcgac ggctttcacc gcagccaccg ctactccgtg gtcagcgacg ccgtggcgtg
      661 ccgctgtgct ggcgtcgggg atgccgcgtc ctacaaggcg gcggtggtca gggtgatcgg
      721 aaatttcgca ggcatccggc gctctaccga cctgatcggg gcaagccgca ggatcccggt
      781 atgagcacga cgccgtatcg cgccgggtcg gccgtcgagg acgacctcag ggagctcgcg
      841 ggagacctcg caaccgcgat tgcgaaggcg cgcgcactga acctgcccac cagcacctat
      901 cttctgcgga tggcgcttgc ggaagtatcg caaaccctga gcgctgaggc ggacgagaac
      961 ggcggtcccg cgcgatagcc tcagcggcga tcgaacctga tcacccggcc tcgttcgatg
     1021 gcgagtgcaa gcttgccgtg cttcagagag agcgcagctt cgccgaacag ctcgcggcgc
     1081 cagccatgca gggcggcgac gtcggcctga tcgtcggccg cgatctgctc gaggtcatcg
     1141 acggtggcga tcaccttgct tgcgaccgca tgacgctccg acgtcatgcg cagcagcact
     1201 ttaagcagct ccacgatcgc gcccccgttc gcgttgttgc gcggcttatc aagcttcggc
     1261 agcgttgccg ggtcgcgcgc caggccgcgc tggacggcgg ccacgatatc cgtgccccat
     1321 ttcgagcggt caaaaccctt tggcaacgaa cgcagattgg cgagccgctc gatcgttgtc
     1381 ggcgcgtggg ttgcgatatc gccgaccgcg tcgtccttca gcacacggct gcgcggcacg
     1441 tcgcggcttt gcgcctcctg ctcgcgccac gccgccacct ccatcagcac ggccaattcc
     1501 ttcggcttgc gtacgcgcgt cttcagccgc tcccaggcgc gttcggggtg aaagtcgtag
     1561 gtcttgggcg aggtcagaat ctccatctcc tcgctgaccc aatcgctgcg gctgcgcttt
     1621 ttcaggtcag catcgagggc gatgaaaacg tcgcgcagat gggtgacgtc ggaaatggcg
     1681 taatggatct gttccttggt cagcggccgg cgcgaccaat ccgtgaaacg gtgggtcttg
     1741 tccgggcggt gaccggtgat gcgctcgacc agttgatcgt aggcaatcga gtcgccatag
     1801 ccgagcacca tggcagccac ctgggtatcg aagatcgggt gcgggacgat gttcgcgcgg
     1861 tgccagacga tttcgatgtc ctgccgagcg gcatgaaaca ccttcaggac cttctcgttc
     1921 gccatcagcg cgaagaacgg tttgagatcg atgccgtcgg ccagtgcatc gatcacgagc
     1981 gcctcgtcgg ggctcgccat ctggaccaca cagagcaggg gatagtaggt ggtctcgcgc
     2041 aggaactcgg tatcgacggt aatgaccggg tgccgggcga ggcgttcaca cgccgcagcg
     2101 agttccgaag tggtggtaat caggtccatg aacgatccat gactctgccc acagccgttc
     2161 cgccgaaagg gctgatatgt caagggtctc ggcgtgaatt cgtggcttgc atttggccga
     2221 gaccgaaaga cgcagccagc cgtccgactc acgtcggcgt cgcggtgcgc cggtgggctt
     2281 tgcgatcagt aacggcgagc gcgcccggac ggcttttggc ttgagggaac tgcgatcacg
     2341 acgacgcaga gcgccagcac cgtcagtccc atgaactcga acacgagagc gtccacgaca
     2401 acatccccaa caaaccggta gctttgtcgg gagcgc
//
LOCUS       DATLJT010001446         4110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4494628,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001446 DATLJT010000000
VERSION     DATLJT010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4494628"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1969
                     /locus_tag="VM910_33220"
     CDS             113..1969
                     /locus_tag="VM910_33220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HVI67403.1"
                     /translation="MPAQAKRAVAQRAPKSPPKSRPPSRLGVLPEWNLADLYGGIDDP
                     QVKRDLDRAAAESLAFEEAYKGKLAALVERPEAGSALVAAVKRYEALDDLLGRLTSYA
                     GLLHAGDTVDPARAKFYADVQERVTAASTHLLFFTLELNRLDDAKLDAAMQDPALAHY
                     RPWIEDVRKDKPYQLEDRVEQLFHEKSVTGYSAWNRLFDETIASLRFKVGSKSLAIEP
                     TLNLMQDPSAATRKAASKALAKTFKENLRTFALITNVLAKDKEISDRWRGFADVADAR
                     HLANRVEPEVVEALVEAVRAAYPRLSHRYYALKARWFGKKRLPHWDRNAPLPAVPTRT
                     IGWSEAQATVLGAYGAFSPEMAAIAERFFKNDWIDAPVRPGKAPGAFSHPTVPSAHPY
                     LLLNYQGRPRDVMTLAHELGHGVHQVLAAPNGALMAPTPLTLAETASVFGEMLTFKKL
                     LANADHKQRKAMLAAKVEDMINTVVRQIAFYTFERKVHSERKSGELTAERLSALWLEV
                     QQESLGPAIELKPGYETFWAYISHFVHAPFYVYAYAFGDCLVNSLYAVYERAAEGFAE
                     RYLQMLAAGGTKHHSELLAPFGLDARDPTFWQGGLGVIERMIVELEGLGQPP"
     gene            2092..>4110
                     /locus_tag="VM910_33225"
     CDS             2092..>4110
                     /locus_tag="VM910_33225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI67404.1"
                     /translation="MTVTRTYRFHEVFEEEVEAVNARRRRFGRAEVALELEPDLNSQP
                     NAHAEGEPIMRPTTGSGLAGLALSGGGIRSASFCLGALQALDNAGVLKSIDYLSTVSG
                     GGYIGTSLSAAMTHAKGDFPFTSSLTQDEPYPVQHIRNHSNYLFPHGSINVFYNVAIY
                     LRGLLANALLILPWLLVLAAITIFFKPDSESLHVSIRGTFPAAFNADHFGATLIALCV
                     FGLLLLIWGLWRSLDITSWEAEIDIGSPWTLVSAIVLILLLFIVFCELQPFVLDGIFK
                     SAARPGGIFSSFVGWLQALAAVLAPFSAAVAFFSRSFGRLLKQGAERPSLAALASRAA
                     GRAAVYIAGAAIPFLLWMAYLYLCFAGIKDVNISGYHAPLWLSDVSQRWFGDKPPFGT
                     PIAWFYLAIGIVLFLLSLLLAPNANSLHRLYRDRLSKAFLFDPTRIEGRRSGTGFKRE
                     SVSVSGLALTDRLKYEDFELAPIDRFKLSQISWIDTPFPLINTALNIQGSKYANRRGR
                     NADFFVFSPKFIGSGATQYVKTKEFEDDVPELDLATAMAVSGAAASSNMGASSIKALT
                     PTLAILNIRLGYWVANPRQLAQDRKPSSVFASVFDQFYFLQELLGLMRESSGTIYLTD
                     GGHIENLGIYELLRRRCELIIAVDAEADPEMSFRSLVALQRYARIDLGV"
BASE COUNT          726 a         1353 c         1209 g          822 t
ORIGIN      
        1 gccggccccg ttgacccgcg gcgatctcgc gccatatcca tcatccatgc gcccggtcgc
       61 gggcgtttct tgctcaccac gtctcgaaat catctctcct tcgcaacctg ttatgcccgc
      121 acaagcaaaa cgcgctgtcg ctcaacgagc gcccaagtcc ccgcccaagt cccgtccccc
      181 gagccgcctc ggcgtgctgc cggaatggaa cctcgccgac ctctatggcg ggatcgatga
      241 tccgcaggtc aagcgcgacc tcgaccgcgc cgccgccgaa agcctggcgt tcgaggaggc
      301 ctacaaaggc aagctcgcgg cgctcgtgga gcgacccgag gcgggatcgg cgctggtcgc
      361 ggcggtgaag cgctacgagg cgctcgatga tcttctcggc cggctcacgt cctatgccgg
      421 cctcctccac gccggcgaca ccgtcgatcc cgcccgcgcg aaattttacg ccgacgtgca
      481 ggagcgcgtc accgccgcct ccacgcatct cctgttcttc acgctcgagc tcaatcgcct
      541 cgacgacgcc aagctcgacg ccgccatgca agatccggcc ttggcgcact accggccgtg
      601 gatcgaagac gtccgcaagg acaagcccta tcagctcgag gaccgcgtcg agcagctctt
      661 tcacgagaag tcggtcaccg gctattcggc gtggaaccgc ttgttcgacg agaccatcgc
      721 cagcctgcgc ttcaaggtcg gctcgaagtc gctcgcgatc gagccgacgc tcaatctcat
      781 gcaggatccg agcgcggcga cgcgcaaggc ggcatcgaag gcgctcgcca agacgttcaa
      841 ggagaacctg cgaacgtttg cgctgatcac caatgtgctc gccaaggaca aggaaatctc
      901 cgaccgttgg cgcggcttcg ccgatgtggc ggacgctcga cacttggcga atcgggtcga
      961 acccgaagtg gtggaggcgc tggtggaagc ggtgcgcgcg gcctatccgc gcttgtcgca
     1021 ccgctattac gcgctcaaag cgcgctggtt cggcaagaag cggctgccgc actgggaccg
     1081 caacgcgccg ctgcccgcgg tccccacgcg caccatcggc tggagcgagg cgcaggcgac
     1141 cgtgctcggc gcctatggcg cgttctcacc cgagatggcg gcgatcgccg agcgcttctt
     1201 caagaacgac tggatcgacg cgccggtgcg cccgggcaag gcgcccggcg cgttctcgca
     1261 tccgaccgtg ccctcggcgc acccctatct gctgctcaac taccagggca ggccgcgcga
     1321 cgtgatgacg ctcgcccacg agctcggaca cggcgtgcat caggtgctgg ccgcgccgaa
     1381 cggcgcgctg atggccccga ccccgctcac gctcgccgag accgcgagcg tcttcggtga
     1441 aatgctcacc ttcaagaagc ttctcgccaa tgccgaccac aagcagcgca aggccatgct
     1501 cgccgccaag gtcgaggaca tgatcaatac ggtggtgcgc cagatcgcgt tctatacgtt
     1561 cgagcgcaag gtccatagcg aacgcaagag cggcgagctg accgccgagc gcctcagcgc
     1621 gctctggctc gaggtgcagc aggagagcct cggtcccgcc atcgagctca agcccggcta
     1681 cgagaccttc tgggcttata tctcgcactt cgtccacgcc ccgttctacg tctacgccta
     1741 tgccttcggc gattgcctgg tgaactcgct ctatgcggtc tacgagcggg cagccgaagg
     1801 cttcgccgag cgctatctcc aaatgctcgc ggccgggggc accaaacacc actccgagct
     1861 gctggcgccc ttcggtcttg atgcccgcga ccccacgttc tggcagggcg ggctcggcgt
     1921 gatcgagcgc atgatcgtcg agctggaggg gctgggccaa cccccgtaag ctgatagata
     1981 atcattttat cgccgagaac cgctcatccg gcgcctctct tgagcggcta atgcggccgt
     2041 gagtaatttg gtaatctact aaccgtcgag aacggctcag tgggggctgc catgacggtc
     2101 accagaacct accgttttca cgaagttttc gaagaagaag ttgaggcggt caatgcccgt
     2161 cggcggcggt tcggcagagc cgaggttgcg ctcgagctcg agcccgatct gaattctcag
     2221 ccgaacgcgc atgccgaggg cgaaccgatc atgcggccga caacgggctc gggtctcgcg
     2281 ggcctggcgt tgtcgggtgg cggtattcgt tcggcgtcgt tttgtctggg ggcgttgcag
     2341 gcgctcgaca acgcgggggt tctcaagagc atcgactatc tctccacggt gtcgggcgga
     2401 ggttacatcg ggacctcact atccgctgcc atgacccacg cgaagggcga ctttcccttt
     2461 accagcagcc tgacccagga cgaaccgtac cctgtccagc acatccgtaa tcattcgaat
     2521 tacttgttcc cgcacgggag catcaacgtc ttctacaatg tcgccatcta cctccgtggg
     2581 ctgctcgcca acgctctact cattctgccg tggttgctgg tcctagcggc catcaccatc
     2641 ttcttcaaac cggattcgga aagcctgcac gtttccatac gcggcacgtt tcccgctgcg
     2701 ttcaatgccg atcatttcgg tgcgacgctg atcgcgctgt gcgtatttgg gctgttgttg
     2761 ctcatctggg ggctctggcg ctcgttggac attaccagct gggaggccga aatcgacatc
     2821 ggttcgccct ggaccttggt gagtgcgata gtgctcattc ttctgctatt tattgtgttt
     2881 tgcgaactgc agccgttcgt gttggatggc attttcaagt ccgccgcccg cccgggcgga
     2941 attttttcaa gctttgtcgg ctggctgcaa gccttggctg ccgtattggc gccattctcg
     3001 gccgcagttg ccttcttcag ccgctcgttc ggccgtctgc tgaagcaggg tgccgagaga
     3061 ccgagcctcg ctgccctcgc gtcgcgcgca gccggacgag cggcggttta catagccggc
     3121 gccgcgatcc cgtttctgtt gtggatggcg tacctctacc tgtgtttcgc gggaatcaag
     3181 gacgtgaaca tcagcggcta ccatgcgccg ctttggctca gtgacgtttc ccagcgttgg
     3241 ttcggagaca agcccccttt cggaacccct atcgcatggt tctatctggc aatcggtatc
     3301 gtactattcc tcctttctct cttgctcgca cccaacgcaa attccctcca ccgtctctat
     3361 cgcgatcggt tgagcaaggc tttcctgttc gatcccacca ggatcgaagg ccggcgttcg
     3421 gggacggggt tcaagcgcga atccgtatcg gtgagcggtc tggcgctaac cgatcggtta
     3481 aaatacgagg attttgaact cgctcccatc gaccgcttca agttgagcca aatctcttgg
     3541 atcgacacgc cgttccctct catcaacacg gccttgaaca ttcaaggatc gaaatacgcc
     3601 aaccgccgcg gtcggaacgc ggacttcttc gttttcagtc ccaaattcat cggcagcggt
     3661 gcgacccaat acgtgaagac caaggaattc gaagacgacg tcccagagct cgacttggcc
     3721 acggccatgg cggtctcggg ggctgcagcg tcgtcgaaca tgggcgccag cagcatcaag
     3781 gcgctgacgc cgacgctcgc tattctcaac atcaggctgg gatactgggt cgccaatccg
     3841 aggcagctcg cccaggaccg caagccttca tccgtattcg cctccgtatt cgaccagttc
     3901 tacttcctgc aggaattgct gggcctgatg cgtgaaagct ccggtacgat ctatctcacg
     3961 gatggcggcc acatcgaaaa tctcggcatc tacgaactcc tgcgacggcg ctgcgagttg
     4021 attatcgcag tcgacgccga ggccgatccc gagatgtcct tccgctcgct cgtggctttg
     4081 cagcgttacg cgcgtatcga tctcggcgtg
//
LOCUS       DATLJT010001447         5082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1047478,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001447 DATLJT010000000
VERSION     DATLJT010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047478"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VM910_33230"
     CDS             complement(<1..191)
                     /locus_tag="VM910_33230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67405.1"
                     /translation="MADKNWQAGGKNLIPARAFLCLVAVATPCHRAFALPLKFASASY
                     IGSLDPDVLWEMLIGGIVV"
     gene            395..1498
                     /locus_tag="VM910_33235"
     CDS             395..1498
                     /locus_tag="VM910_33235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67406.1"
                     /translation="MDRVDCVVVGAGVIGLAVARRLAQAGREVVVLEAAEGIGTVTSS
                     RNSEVIHAGIYYKAGSLMARTCVAGKKMLYRYCADHGVPHKNCGKLIVATTPKEAEKL
                     QSIRAHAEANGVLDMQMLSGEAARALEPALNCDAALLSPSTGIIDSHAFMLALRGDAE
                     AAGAALAFHTPLLRARAVAGKIELDAGGDAPMTLECRLLVNSAGLNAPAVARGIDGMP
                     LASIPRAYLAKGNYFSCSRKAPFSHLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWVD
                     TIDYAVDPARAARFYPAIRKYWPALPDGALMPSYSGMRPKIVPPAVASQDFVIQGPKD
                     HGVAGLINLFGIESPGLTSSLAIADYVGELAGA"
     gene            complement(1627..1815)
                     /locus_tag="VM910_33240"
     CDS             complement(1627..1815)
                     /locus_tag="VM910_33240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HVI67407.1"
                     /translation="MAIQAHLVELERKHKSLENELHEALIHLSTDDLQIAELKRRKLM
                     VKDQIERLKHAAADETVH"
     gene            2116..2322
                     /locus_tag="VM910_33245"
     CDS             2116..2322
                     /locus_tag="VM910_33245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HVI67408.1"
                     /translation="MTDEDERELHAELARLQQEHRDLDAAIDALHHSPAPDLLRLQRL
                     KKRKLQLRDRIAFIEDQITPDIIA"
     gene            2487..2699
                     /locus_tag="VM910_33250"
     CDS             2487..2699
                     /locus_tag="VM910_33250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67409.1"
                     /translation="MSSTSSPFDNSHYEKACDQAIAMCDGNLRSTIKALIMANEYLEA
                     ELLELQAAISSGCAPLPSSGCESSAA"
     gene            2712..2963
                     /locus_tag="VM910_33255"
     CDS             2712..2963
                     /locus_tag="VM910_33255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67410.1"
                     /translation="MADITYYVAMPFLEDDNGCPIAGVAEECQSPATALRRAEILSRG
                     AGHIGAVAFSRTGDPMVGEFGDAKLIRTFGNVPDDLSGL"
     gene            complement(2950..3657)
                     /locus_tag="VM910_33260"
     CDS             complement(2950..3657)
                     /locus_tag="VM910_33260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVI67411.1"
                     /translation="MFMKKKTGAGTRGAFKRPRRGATASKTERPRVAPRPSLSADHAK
                     ATIRRLRSDLTQALKRIDELQASAETDFLLGIPNRRGFERELHRAIAYIKRYRANGAL
                     IVLDVDRLKPINDAFGHAAGDQVLKAIADVLARQVRASDVVGRLGGDEFALLLWNLSE
                     TDARAKAASLEEAIDRLMFVFDGRDVTAGASAGVAVLGSNAEATRALEEADRAMYVRK
                     AQRRHELEDAAQQLTAR"
     gene            3909..4397
                     /gene="purE"
                     /locus_tag="VM910_33265"
     CDS             3909..4397
                     /gene="purE"
                     /locus_tag="VM910_33265"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVI67412.1"
                     /translation="MTAPVAIIMGSQSDWETMRHAVDTLTELGVACDTRIVSAHRTPE
                     RLYAFAKGAKGEGFKVIIAGAGGAAHLPGMTAAMTELPVLGVPMESQAMSGVDSLYSI
                     VQMPAGVPVGTLAIGKAGAINAALLAASVLALSDAALGERLATWRRQKSEAVAERPEG
                     SA"
     gene            4394..>5082
                     /locus_tag="VM910_33270"
     CDS             4394..>5082
                     /locus_tag="VM910_33270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVI67413.1"
                     /translation="MTASNRVKLKPGDTIGILGGGQLGRMLAMAAARLGLRCQVFSPD
                     PDSPAFDVVLNATCAEYADVEALELFANDVDVITYEFENVPAAAAMILAARRPVLPDR
                     KILETTQDRLAEKDFIKRLGIGTADYADVSTTAGLLAAVARIGLPAVIKTRRFGYDGK
                     GQALIRAGDDLDRVWTDLGTKSAILEAFIPFEREISVIAARSADGHVECFDVTENEHR
                     DHILKTSRVPAA"
BASE COUNT          938 a         1592 c         1569 g          983 t
ORIGIN      
        1 cagacgacaa tcccgccgat cagcatctcc cagagcacgt cgggatcgag cgaaccgata
       61 tagcttgcgg acgcgaattt gagcgggagt gcgaaggctc gatggcatgg cgtcgcgacg
      121 gcgaccaggc acaagaaggc ccgcgcagga atcagattct tgccgcctgc ctgccaattc
      181 ttgtcagcca tcagtcaccc gcggatttca acctccgagt gtctggcttc acgcgtttgg
      241 atcaggtaaa cgcgtaccct tgcggcccaa aaatccggct caaattaagg caaatggcct
      301 gacatgatta ccgcttcgtt aatggcaatt ctgcctggga gggccttctc catcggacga
      361 tctccaaagc tccgcacgag gcgccggaac acgcatggat agggtcgatt gcgtggttgt
      421 cggggccggc gtgatcggtc ttgccgtggc gcgtcggctc gcgcaggccg gccgtgaagt
      481 ggtcgtgctg gaggccgccg agggcatcgg caccgtgacc tcgtcccgca acagcgaggt
      541 gatccatgcc ggcatctact acaaggccgg aagcctgatg gcgcggacgt gcgtcgccgg
      601 caagaaaatg ctctaccggt actgcgccga ccacggcgtg ccgcacaaga actgcggcaa
      661 gctgatcgtc gccacgacgc cgaaggaagc cgagaagctg caatcgatcc gcgcgcacgc
      721 cgaggccaac ggcgtgctcg acatgcagat gctttccggc gaagccgcgc gcgcgctcga
      781 accggcgctc aattgcgacg cggcgctatt gtcgccttcg accggcatca tcgacagcca
      841 cgcattcatg ctggcgctac gaggcgatgc agaggccgca ggcgcggccc tggcctttca
      901 tacgcctctg ctgcgcgcca gagctgtcgc gggcaagatc gaactcgacg ccggcggcga
      961 tgcgccgatg acgcttgaat gccggcttct cgtcaattcc gccggcctga acgcgccggc
     1021 cgtggcgcgc ggcatcgacg gcatgccgct tgcgtcgatc ccccgcgcct atctcgccaa
     1081 gggcaactat ttcagttgca gcaggaaggc tccgttttca catctgatct atccggtgcc
     1141 cgaaccgggc ggccttggcg tgcacctcac cctcgacatg gcgggacagg ctcgcttcgg
     1201 ccccgatgtc gaatgggtcg acaccattga ctacgcggtc gatccggcgc gggccgcgcg
     1261 tttctatccg gcaatccgca agtactggcc ggcgctgccc gatggcgcgc tgatgccgag
     1321 ctattcgggc atgcggccaa agatcgtgcc gcccgcggtc gcctcgcagg atttcgtcat
     1381 tcagggtccg aaggaccacg gcgttgccgg actgatcaac cttttcggaa tcgagtcgcc
     1441 ggggcttacg tcatcgcttg caattgccga ttacgtcggc gagctcgcgg gcgcatgaca
     1501 acctgcggcg cggtcttgcg cggccctgga ttcgaacccg tggaaaagcc ccgccaaaat
     1561 gggcgcaagc gcgcccatca ggcgattatg gcaacgtgac agtctgactg actgatttag
     1621 tgggggctag tgcaccgttt cgtcggcggc ggcgtgcttg agccgctcga tctggtcctt
     1681 aaccatcaat ttccggcgct tcaactcggc tatttgcagg tcgtccgttg aaaggtggat
     1741 gagcgcttca tgcagttcgt tttcgaggct cttgtgtttt cgttccagtt caacgagatg
     1801 tgcctgaatg gccattcgat acctcctcgg ctaagttcaa cctcagattt gatccggaag
     1861 aatgagtgta cacctccgac cgaatctgtc gatggttaag cgaatcggca ccgtcatttt
     1921 ttgacggcct tatgtaacga accgtgagca gggaacccat gatcttcagc atccgccggg
     1981 cggtgagttt gcctgccgct gcagtgattt ggacgcactt tgcccgcttg cgccgcgcct
     2041 cgtcagggac gatatttccc acagggtttc cacagccctt cgcaatctca tcgcgtgttt
     2101 cggctaacgt acatcatgac cgatgaagat gagcgcgagc tgcatgccga gctggctcgg
     2161 ctgcagcagg agcaccgcga tttggacgcg gcgatcgatg cgctgcatca ttcgcccgca
     2221 ccggatcttt tgcggttgca gcggttgaag aaacgcaagc tgcagctacg cgaccgcatc
     2281 gctttcatcg aagaccagat cacgcctgac atcatcgcct agttcatgtg tgccgcgcgt
     2341 tgctgtgcgg cgtgaaaatc cggccgggcg gtcgtgaaat ctgcttgact cgaagggaac
     2401 aaaaagagaa catcggagca cgtcacgact gtaaacctct tcgctgtaaa cccatcgccg
     2461 ccttcaaacc gcatcgggat tgcgtcatgt cctcaacctc atctccgttc gacaacagcc
     2521 attacgagaa ggcctgcgac caggcgattg cgatgtgcga tggcaatcta cgctcgacca
     2581 tcaaggcgct gatcatggcc aatgaatatc tggaagcgga gcttctggaa ttgcaggctg
     2641 cgatctcgtc cggctgcgcg ccgctgccct catccggctg cgagagcagc gccgcgtgaa
     2701 gagaaaaaat catggcggac atcacatact acgtggcgat gccgtttctt gaagacgaca
     2761 acggctgtcc catcgcaggc gttgcggaag aatgccaaag tccggcgacc gcgctgcgcc
     2821 gagccgagat cctgtcgcga ggcgcggggc atatcggcgc cgtcgctttc agccgcaccg
     2881 gcgatcccat ggtcggcgag ttcggagatg caaagctgat caggacattc ggcaatgtac
     2941 cggatgatct cagcgggctg tgagctgttg cgcggcgtct tccagttcgt gccgccgttg
     3001 agccttgcgt acatacatgg cgcggtcggc ctcttcgagc gcgcgagtag cctctgcgtt
     3061 cgaacccaga accgctacgc ctgcggaagc acctgccgtc acatcgcgtc cgtcgaacac
     3121 gaacatgagc cgatcgatcg cctcctccaa tgacgccgct ttggctctcg catcggtttc
     3181 gctgagattc cacaacagaa gggcgaactc gtcgccgccc agccgcccca ccacgtcaga
     3241 ggcgcgcacc tgacgggcga gcacgtccgc aatcgccttg agcacctgat cgccggcagc
     3301 atgcccgaag gcatcgttga tcggcttcaa gcggtcgacg tcgagcacga tcagggcgcc
     3361 gttcgcccgg tagcgcttga tatacgcaat cgcacgatgg agttcgcgct cgaagccgcg
     3421 ccggttcggg atgccgagca ggaaatcggt ttccgccgac gcctgcagtt cgtcgatgcg
     3481 ctttaacgct tgcgtcaggt cgctccggag ccggcggatg gtggctttgg cgtggtcggc
     3541 cgacagcgac ggccggggcg caacgcgcgg ccgctccgtt ttgcttgccg tcgcgccgcg
     3601 ccgcgggcgt ttgaaggccc cccgcgttcc cgccccggtt ttctttttca tgaacatcct
     3661 tgttgaggcc cgtagtgtcc cgaatccgaa gttcgcatca tcttgcgtct tttgcgaagt
     3721 tcggattcga aaggacacgc aaaacttgtg attccggtgc ggttttggtt cggaaccgcc
     3781 acaccaggct tgccggattt aaccaagaca ggatagtgca ttcctattcc cttgccacgc
     3841 cttgccgccg tcaggcccga aactataatt gggcctatgc ttctggattc ccgagaagaa
     3901 ttgagcttat gaccgcaccc gtcgccatca tcatgggaag ccagtcggac tgggagacca
     3961 tgcgccatgc ggtggacacg ctgaccgagc tcggcgtcgc atgcgacaca cggatcgtct
     4021 cggcccatcg cacaccagag cggctttatg ccttcgccaa gggcgccaag ggcgagggct
     4081 tcaaggtcat catcgccggt gccgggggcg cggcccatct gccgggaatg accgctgcaa
     4141 tgacggaact gccggtactc ggcgtgccca tggaatcgca ggcgatgtcg ggagtggatt
     4201 cgctttattc gatcgtgcag atgccagcgg gtgtaccggt cggaacgctt gccatcggca
     4261 aggccggtgc catcaatgcc gccctgcttg ccgcgagcgt cctggcgctc agcgatgcag
     4321 cgctcggcga gcggctcgcc acctggcgcc gacaaaaaag cgaagcggtc gccgagcgcc
     4381 cggaggggtc ggcgtgacag catccaaccg ggtgaagctg aagccgggcg acaccatcgg
     4441 aattctcggc ggcgggcaat tgggccggat gctggccatg gcggcggcac ggcttggctt
     4501 gcgctgtcag gtgttctcgc cggatccgga ttcgccggct ttcgacgtgg tgcttaacgc
     4561 gacctgcgcg gaatatgccg atgtcgaagc gctcgaattg ttcgcaaacg acgtcgacgt
     4621 catcacctat gagttcgaga acgttcctgc cgcggcggcg atgatcctcg ccgcccggcg
     4681 ccctgtgctg cccgaccgca agattctcga aacgacccag gaccggctcg ccgaaaagga
     4741 tttcatcaag cggctgggca tcggcaccgc cgactatgcc gacgtgtcga cgacagccgg
     4801 cctgctcgcg gcggtggcgc gtatcgggct gccggcggtg atcaagacgc gccgcttcgg
     4861 ctacgacggc aagggccagg cactcatccg tgcgggcgac gatctggacc gcgtctggac
     4921 agatctcggc accaagtctg cgatcctcga ggccttcatt cccttcgagc gcgagatttc
     4981 ggtgatcgcg gcgcgctcgg ctgacggcca tgtcgaatgt ttcgacgtta ccgagaacga
     5041 gcaccgtgat cacattctga agacctcgcg cgtgccggcg gc
//
LOCUS       DATLJT010001448         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2427332,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001448 DATLJT010000000
VERSION     DATLJT010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427332"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1543)
                     /gene="fumC"
                     /locus_tag="VM910_33275"
     CDS             complement(137..1543)
                     /gene="fumC"
                     /locus_tag="VM910_33275"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVI67414.1"
                     /translation="MSNTRIESDALGNIEVPADKLWGAQTQRSLKYFSIGRELMPREM
                     ITAYAVLKKACANANFSGKRLPAEIHKLIVQACDEILGGKHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCCQLAGTPLGSKKPVHPNDHVNMSQSSNDNFPSAMYIAAATNTVQRL
                     IPSVSALQEAIAARAVEWDDIVKIGRTHLQDATPITLGQEWSGYAGMLSDNLARINDA
                     LRDVYQLALGGTAVGTGINAAPGFAEMAAAEIAALTRLPFVSAPNKFVVQGAHDALVQ
                     LSGTLRTLAGSLYKIANDIRLLASGPRAGLAELILPENEPGSSIMPGKVNPTQAEALT
                     MIAVQVMANDVAIGFGGAGGHLEMNVYKPLMINGIMQSITLLADGCDNFRKFLIDGTR
                     PNTRKIEEHVKRSLMLVTALAPVIGYDKASKIAHHAAEHDLTLKEAALQLGFVDEATF
                     DRVVDPKKMVRPYVASGR"
     gene            1789..2445
                     /gene="bluB"
                     /locus_tag="VM910_33280"
     CDS             1789..2445
                     /gene="bluB"
                     /locus_tag="VM910_33280"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HVI67415.1"
                     /translation="MVRETRHPPQFDAAFRAQFAELVRWRRDVRRFRSACVPPELVSQ
                     LLELASHAPSVGLSQPWRFVLVETPERRGAIRDNFARANKAALDGYQGERQARYARLK
                     LEGLSQAPVHLAVCADEATAQGAGLGRQTMPETIRYSVVAAIQTLWLAARAEGLGVGW
                     VSILDPAEIKRTLDLPADWSFIAYLCLGWPEEEHADPELERHGWEQRRCEASVKPLLR
                     "
     gene            complement(2442..>2710)
                     /locus_tag="VM910_33285"
     CDS             complement(2442..>2710)
                     /locus_tag="VM910_33285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVI67416.1"
                     /translation="PIAKPGLAVAFILAVIFSWNNFVFGIVLAGRETRTLPVAVYNMI
                     SFDQLSWGPLAAAALIVTFPVLLLTMFAQRQIVAGLTAGAVKGG"
BASE COUNT          478 a          805 c          890 g          537 t
ORIGIN      
        1 gcgggagcgc agagtcgtaa ctcgcggcgt ttccttcgaa acggggcctg cggccgtccc
       61 tcctcatgga gaggaggtgt cgaaccatgc ggtccgagtg gctgtttgac attcaaatca
      121 gaacggtgtg tggccgctaa cggccgcttg ccacatacgg ccggaccatc ttcttcggat
      181 caaccacgcg atcgaaggtg gcctcgtcga caaagcccaa ctgcagcgcg gcctccttca
      241 gcgtgagatc gtgctcggca gcgtgatggg cgattttcga ggccttgtcg tagccgatca
      301 caggtgcgag cgcggtgacg agcatcagcg atcgcttcac gtgctcctcg atcttccggg
      361 tgttcggtct cgtgccatcg atgaggaatt tgcggaagtt gtcgcagccg tcggcgagca
      421 gcgtgattga ctgcatgatg ccattgatca tcagcggctt gtagacgttc atctcgagat
      481 ggccgccggc gccgccaaac ccgatcgcga catcgtttgc catcacctgc acggcgatca
      541 tggtgagtgc ttcggcctgc gtcgggttga ccttgcccgg catgatcgag gagccgggct
      601 cgttctccgg cagtatcaat tcagccaggc ccgcgcgcgg accggacgcc agcaggcgga
      661 tgtcgttggc gatcttgtag agcgagccgg cgagggtacg taacgtgccg gagagctgca
      721 ccagcgcatc atgagcgccc tgcacgacga acttgttggg cgcgctgaca aagggaagcc
      781 ttgtcaatgc ggctatctcg gctgcggcca tctccgcaaa gcccggcgcg gcattgatgc
      841 cggttccgac cgcggtgccg ccgagtgcga gctggtagac atccctcaat gcgtcgttga
      901 tccgtgcgag attgtccgaa agcatgccgg cataacccga ccattcctgg cccagcgtga
      961 tcggggttgc atcctgcaga tgagtgcggc cgatcttgac aatatcgtcc cactcgacgg
     1021 cccttgcggc gatggcctcc tgcagagcac tcacggacgg gatcaggcgc tgtacggtat
     1081 tcgtcgccgc ggcgatatac atcgccgatg gaaagttgtc gttcgacgac tgcgacatgt
     1141 tgacgtgatc gttcgggtgc accggcttct tgctgccgag cggcgtgccg gcgagctggc
     1201 agcagcggtt ggagatcacc tcgttgacgt tcatgttgaa ctgtgtgccg ctgccggtca
     1261 tccagacatg cagcgggaac atgtcgtggt gcttaccccc caggatttcg tcgcaggcct
     1321 ggacgatcag cttgtgaatc tcggcgggga gccgtttgcc cgaaaagttg gcgttggcgc
     1381 aggctttttt caggacggcg taggccgtga tcatttcgcg cggcatcaat tcccggccga
     1441 tcgagaaata tttgagggaa cgttgcgtct gcgcgcccca caatttgtcg gcggggactt
     1501 cgatattgcc gagggcgtcg gattcgatgc gggtattgct catgtcgctg gtcctgttgg
     1561 caggtaattg gaacgagggt accacggtgg ggtttgcttg acaggctggt gcggcggatt
     1621 tgacattggc ttccgtccgc cgcaaatcct tgtggcaaat ggcaaatcca aagccacatg
     1681 agaatcaaat atttgctagt gtccctttgg ttccaggatt cgcaaaagtg cccgccgcga
     1741 aatcatgcga atgttagaac ctccggctga acggtgtttt ggcgaagcat ggtgcgagaa
     1801 acccggcatc caccgcagtt cgacgccgcc ttccgcgcgc agtttgccga actcgtgcgc
     1861 tggcggcgcg acgttcgccg gtttcggtcc gcctgcgtgc cgcctgagct cgtttcgcaa
     1921 ttgctggagc tcgcctcgca tgcgccgtcc gtgggcttga gccagccttg gcgcttcgtc
     1981 ctcgtggaaa cgcccgagcg gcgcggagcg atccgcgaca attttgcgcg cgccaacaag
     2041 gcagcgctcg acggctatca aggcgaacgc caggcgcgct acgcgcggtt gaagctagag
     2101 ggcctttcgc aggcgcccgt gcatcttgcc gtctgcgcgg atgaagcgac cgcgcaaggt
     2161 gccggcctcg ggcgccagac catgccggaa accatccgct attccgtggt cgcggcgatc
     2221 cagacgctat ggcttgcggc gcgcgcggaa gggcttgggg tcggctgggt gtcgatcctc
     2281 gatcccgcgg agatcaagcg cacgctcgat cttccggccg actggagttt tatcgcctat
     2341 ctctgcctcg gatggcccga ggaggagcac gcggatcccg agctcgagcg gcacggctgg
     2401 gagcagcggc ggtgcgaggc ttcggtcaag cccttgctgc gctagccgcc cttgacggcg
     2461 ccggccgtga ggcccgccac gatctggcgc tgcgcaaaca tcgtgagcag caggaccgga
     2521 aatgtcacga tcagcgccgc ggcagcgagc gggccccagc tcaactgatc gaaggagatc
     2581 atgttgtaga cggcaaccgg cagcgtccgg gtctcgcggc cggcgagcac gatgccgaac
     2641 acgaaattgt tccaggagaa gatcacggcg agaatgaagg ccaccgcaag accgggcttt
     2701 gcgatcggca
//
LOCUS       DATLJT010001449         5079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_359110,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001449 DATLJT010000000
VERSION     DATLJT010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359110"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VM910_33290"
     CDS             complement(<1..279)
                     /locus_tag="VM910_33290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67417.1"
                     /translation="MTAPLTPIDRAVALLFDAAVSYASGDALALAVTRGKLVAIAGTI
                     EDFEAQLTTAVVELIAPRCPNIPHDEIEHAVLVDVRRHVSRSVFLAEFP"
     gene            complement(320..895)
                     /locus_tag="VM910_33295"
     CDS             complement(320..895)
                     /locus_tag="VM910_33295"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI67418.1"
                     /translation="MRARIPIATTPTERRVRREARSETLRELALALRQDGWTYAHIGK
                     ALGVSLERARRVIKKAERLRDQPRWHDKLPVRALNFLLKHELESLPEIDAARAVALLS
                     RRELLGFPNLGVGTCAAVVAWLSHHGLKLQPPQSSRRLVAQCVAERDAASPDHENEAG
                     TPMGCPSVSNDPFSADRNDEQAPCLYPPPAT"
     gene            complement(964..1521)
                     /locus_tag="VM910_33300"
     CDS             complement(964..1521)
                     /locus_tag="VM910_33300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67419.1"
                     /translation="MSGRGRTMRRRGRPKGTTVPLDRDPQRFEIAVWHGIHLDGHGPY
                     TSAHWAAWVMSRKPIKLEDVEGLLTVAGTEIEFTASSLDKHIDRLARKAESIPVDGDP
                     WLHMSALAIRAFILAQRTRNMEMACYLLDVLIGLGWREVIERLCARIDDMLKSNIPPR
                     EGKLGREGQALLNWLRSVTKGEKPE"
     gene            complement(1755..2084)
                     /locus_tag="VM910_33305"
     CDS             complement(1755..2084)
                     /locus_tag="VM910_33305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67420.1"
                     /translation="MANVATLPPRDPIFALAPELRLHQSKLDGQIAVNSREVARVFLR
                     DDHRQLICHIMWSIGNRPMQPNYHDEFQQRPDGTVDITSRGLLSALCHWGFEYEETER
                     HREFMHA"
     gene            complement(2077..2556)
                     /locus_tag="VM910_33310"
     CDS             complement(2077..2556)
                     /locus_tag="VM910_33310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67421.1"
                     /translation="MVSPLHIATVGGHPLRFFCTPLDDGRPDLPWVAIDDLGRCLGLS
                     RAHRRIYLAVFYRVWKEAVRTVATPDGRVSIVPHTMARASVEALIEDNGWAPASARDE
                     YVSASAGAMMKLPRGPFHSAEALRAWVNAAMNRWSISGHTVSMAELFPFITTDRADG"
     gene            complement(2556..3197)
                     /locus_tag="VM910_33315"
     CDS             complement(2556..3197)
                     /locus_tag="VM910_33315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67422.1"
                     /translation="MDAGLIRQVGERRDDDTGKMRPVYQFIKTAENKSKWIETLCALP
                     SDVLDAFENSLGEGAVDATDETVDWFEGKPECRRTDMVDKAEVSRQLRALVEERLFGN
                     GPWRSDDRAVNQAVHDKLCAWGLQTVDLTQTIRTTELGSELQVELMTVFMAHHVPYEA
                     PDILRSFGLITPMECDELTTRFNEDGERPEDVLPPLLRRLWRQYFNRDAPCEQ"
     gene            complement(3289..3555)
                     /locus_tag="VM910_33320"
     CDS             complement(3289..3555)
                     /locus_tag="VM910_33320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67423.1"
                     /translation="MFIMHMPESFKIGDSADCRINGEPTRLTWRDKNTLVIGENDART
                     IVTTHIENGLRCFICGDADNTRYGVEHVPGGEGFIVFAKPDNKR"
     gene            complement(3595..4020)
                     /locus_tag="VM910_33325"
     CDS             complement(3595..4020)
                     /locus_tag="VM910_33325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67424.1"
                     /translation="MSDPFDLSYKPTPDLPDDEGKALLKHLETLWAEIDRAFSDKKLK
                     RIRNSLAPLRQDETTLRKALLDAAVRFSTHENFTTWLCRNHCEHIEAYDPLRMTNEVL
                     DAIHELRWKMQCLEDNMEGVDKIIKALVEERLSKPKQQE"
     gene            complement(4207..4635)
                     /locus_tag="VM910_33330"
     CDS             complement(4207..4635)
                     /locus_tag="VM910_33330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67425.1"
                     /translation="MTTHVPTSCRDGDLPPDLAEFVRQHGDWRRTPAEQWATYTPEQQ
                     DLMRRCGAYSLVTAEEWREWDRLNAEWQDRRRRIRPPANEFLRQLPKPEPSPFETCAT
                     CGREAQFGYRDAASGGLVWFCAEHRRAKWWADARRDPETF"
     gene            4828..4986
                     /locus_tag="VM910_33335"
     CDS             4828..4986
                     /locus_tag="VM910_33335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67426.1"
                     /translation="MVLAGLFLVFPSLLEALMEAVIGRDIDYTATFGLAIGAAVLLRQ
                     RMTLATPR"
BASE COUNT          906 a         1553 c         1548 g         1072 t
ORIGIN      
        1 cggaaactcc gccaaaaaga ccgagcggct aacgtgccga cggacatcga ccaggaccgc
       61 atgctcgatc tcgtcgtgcg gaatgttcgg gcagcgaggc gcaatcagtt caacgaccgc
      121 cgtcgtaagc tgagcttcga aatcctcgat agttccagcg atggcgacta gcttgccgcg
      181 cgtgactgcc agggccaatg cgtcgccgct ggcgtaactc accgccgcat caaacagcag
      241 cgctacggca cggtcgattg gggtgagggg cgcggtcatg acgccccctg cggcagagcg
      301 ttcgggtcga ttacttgcgt caggtcgctg gtggcgggta tagacatggc gcttgctcat
      361 cattgcggtc cgctgagaac ggatcgttgg aaacggacgg gcaccccata ggggtgcccg
      421 cttcgttttc gtggtcaggg gatgctgcat cgcgctcggc gacgcattgc gcgactagcc
      481 gtcggctgct ttgaggtggt tgcagcttta gcccgtggtg tgagagccaa gcgacgaccg
      541 cagcgcaggt gccaaccccg agattcggga agcccaatag ctcgcggcgc gagagaagcg
      601 cgaccgcacg tgccgcgtcg atctcgggca agctctccag ttcgtgcttg agtaagaaat
      661 ttagcgcccg caccggcagt ttgtcgtgcc agcgcggctg gtcacgcagc cgttcggcct
      721 ttttgatgac ccggcgggct cgttcgagcg agacaccgag cgccttgccg atatgggcgt
      781 aagtccaacc gtcttggcgc agcgccagcg ccagctcgcg cagcgtttcg gagcgtgctt
      841 cgcggcggac gcgccgttcg gtgggagtcg tcgcgatggg gatgcgggcg cgcatcctca
      901 ttgtaagaca gaacgcaaaa aatttctcta cccgtaaata attgggtagg taaccctcgc
      961 gaactactcg ggtttttcgc cctttgttac gctgcgtagc cagttgagga gcgcttgccc
     1021 ctcgcgtcca agcttgccct cacgcggtgg aatattgctt ttcagcatgt cgtcgatgcg
     1081 agcacacaaa cgctcaatca cctcacgcca gccgagccca atcaggacat cgagcaggta
     1141 gcatgccatt tccatatttc tggtgcgctg cgcgaggatg aacgccctga tcgccagggc
     1201 cgacatgtga agccagggat cgccgtcaac cgggatgctc tcggccttgc gtgcgagccg
     1261 atcgatgtgc ttgtcgaggc ttgacgccgt gaactcaatc tcggttcccg cgaccgtcag
     1321 taatccttcg acgtcttcga gcttaatcgg tttacggctc atcacccatg ccgcccagtg
     1381 ggcactcgta taaggtccgt gaccgtcgag atgaatgcca tgccacactg cgatctcaaa
     1441 cctttgcggg tcgcgatcga gcggaacagt cgtgcccttc gggcggccgc gcctcctcat
     1501 ggtccgcccg cgaccgctca tcgacgcctc cactcacgga acgcctcggg ctccgcgacc
     1561 gtggcccaga gttcgtcgtg cagaattgga cctgctggta agggctgggt gcaatcctga
     1621 cagcattcac gcaattcgct tccaacgtac ttgaagcaac gcaaaggacc gaagagttcc
     1681 tgcatctttt gatgcatggg attgacgccg gtcttggctt cgatttcgcg catcctcgcg
     1741 gagatcaggt cgacctaagc atgcataaac tcacgatgac gctcggtttc ctcgtactcg
     1801 aatccccaat ggcagagcgc cgacagcaag ccgcgcgatg tgatgtcgac ggtgccatcc
     1861 gggcgctgct ggaattcatc gtgatagttc ggctgcatcg gacggttccc gatgctccac
     1921 atgatgtggc atatcagttg ccgatggtcg tcacgaagga acacgcgcgc cacctcgcgg
     1981 gaattgaccg cgatctggcc gtcgagtttg gactgatgca gccgcaactc cggtgcgagc
     2041 gcgaagatgg gatcgcgcgg aggtaaggtg gcgacgttag ccatcggcgc ggtccgtggt
     2101 tatgaaagga aatagctcgg ccatagaaac ggtgtgtccg gagatgctcc aacggttcat
     2161 cgcagcgttc acccacgccc tgagtgcctc ggcactatgg aagggccccc ggggaagctt
     2221 catcatcgcg ccagcgctgg cgctgacata ttcgtcgcgt gcgctcgcgg gtgcccaccc
     2281 attatcttcg atcagggcct cgaccgaggc ccgcgccatc gtgtggggaa caatgctgac
     2341 gcgaccgtct ggcgtcgcga ccgttcgaac ggcctccttc cagactcgat aaaacacagc
     2401 cagataaatt cgccgatgag cgcgactgag cccgaggcat cggcccaaat catcaatcgc
     2461 aacccacggc agatcgggcc gcccgtcgtc gagcggcgtg cagaagaagc gcagtggatg
     2521 cccgccgacc gtggcgatgt gcagcggtga gaccatcatt gctcacacgg agcgtcgcgg
     2581 ttgaaatact ggcgccagag ccggcgcagc agcggcggca gcacgtcctc gggccgctcg
     2641 ccatcctcgt tgaagcgcgt ggtcaactcg tcgcattcca tcggggtaat cagaccaaag
     2701 ctccgcaaga tatctggcgc ctcgtatggc acgtggtgcg ccatgaaaac ggtcatgagc
     2761 tcgacctgta gctcgctgcc gagctcggtc gtgcggatgg tttgcgtgag gtccaccgtc
     2821 tgcagccccc aggcgcacag cttgtcgtgc accgcctggt tgaccgcccg gtcgtcgctg
     2881 cgccacgggc cgttcccgaa aaggcgctcc tccaccagcg cgcgtagctg gcgactgact
     2941 tctgctttat ccaccatgtc cgtcctcctg cattctggtt ttccctcgaa ccagtccact
     3001 gtctcgtcgg tggcatcgac ggcgccttcg cccagactgt tttcgaaagc gtcgagcacg
     3061 tcgctgggga gggcgcagag cgtttcgatc catttgctct tgttctcggc ggtcttgatg
     3121 aactgatata ccggcctcat cttgccggtg tcgtcgtctc gccgctcacc gacttgccgg
     3181 atcaggccgg cgtccaagag agcgtccgcc gcgcgcgcaa ggcgcggatc gatatcattc
     3241 aaaatcgcgg caacgtgttt ttcccgcgtc caagtcatag cttccccctc agcgcttgtt
     3301 atcgggcttg gcaaaaacga tgaagccttc gcccccaggg acgtgttcga cgccgtatcg
     3361 ggtgttgtcg gcatcaccgc aaatgaagca acgcaatcca ttttcgatat gcgttgtcac
     3421 aatggtgcgg gcatcgttct cgccgatgac caaggtgttc ttgtcgcgcc aggtcagccg
     3481 cgtcggctcg ccattgatgc ggcagtcggc gctgtcgccg atcttgaagc tctccggcat
     3541 atgcatgatg aacatggtct cctcctcacg ttgttttggg gcgctcgcga cggttcattc
     3601 ctgttgcttc ggtttagaaa gccgctcttc gaccagtgct ttgataattt tgtccacacc
     3661 ctccatgttg tcttccaggc actgcatctt ccatcgcagc tcgtgaatcg catcgagcac
     3721 ttcgttggtc attcgcagcg ggtcgtaggc ctcgatgtgt tcgcagtgat tgcgacagag
     3781 ccaagtcgtg aagttttcat gggtgctgaa acggacggcg gcgtcgagca aggcctttcg
     3841 cagggtcgtt tcatcctgcc ttagcggcgc aagtgaattc cggattcgct ttagcttctt
     3901 gtcgctgaag gcccggtcta tctcagccca taacgtttca agatgtttca gcagcgcctt
     3961 gccttcatcg tccggcagat cgggcgtcgg cttgtaagat agatcgaacg gatcactcac
     4021 cttgtcactc ctttggttgc gcctcggcgc ggcggcggtc gcggctggac gcggatgaag
     4081 gcgagctgtc gaatcagctc gcgatcgcgc ggcggcgtta gagcgaattc gtccataacg
     4141 agcctcgcca ttgcgtcgtg gcgatgatcc catccgagcc gatagcgcag cgcttccggt
     4201 acgatttcaa aacgtttctg ggtctcgtct tgcatctgcc caccatttcg ctcggcgatg
     4261 ctcggcgcag aaccacacga gcccaccgga tgcggcatcg cgatagccga actgagcctc
     4321 ccgcccgcat gtggcgcagg tttcgaacgg cgagggctcg ggtttcggca gttgtctgag
     4381 gaattcattc gcgggcggac ggatgcgtcg gcggcggtcc tgccattcgg cattgagccg
     4441 gtcccactcg cgccattctt cggcggtcac gagcgagtag gcgccgcaac ggcgcatcag
     4501 atcctgttgc tctggtgtgt aggtcgccca ttgttcggct ggcgtgcggc gccaatcgcc
     4561 atgctgacgc acgaattcag ctaagtccgg gggcagatcg ccatcgcggc aggacgtggg
     4621 gacgtgagtc gtcatcacgc gcccccgcga acagagggcg aatcgtcacc agttttttca
     4681 ccagtcttcg gtggcgttct gtagtttttg gtgctcttcc gttcccttgt cgcgcctgcc
     4741 gtggaattta tcgactgtcg ataaactccc atttcaccgg catttctgcg cgctcgcgcc
     4801 gggtccgaaa aataggaacg cctgctgttg gtactggcgg gcctatttct ggtatttccg
     4861 agcctgctcg aggcgttgat ggaagcggtc atcggccgtg acatcgacta caccgcgaca
     4921 tttggcttgg cgatcggcgc cgcggtcctg ctaagacagc gcatgacgct ggccacgccg
     4981 cgctgacccg cggcgggcgc gccataggga gaggacgacc atgaaacgca aaggactcgt
     5041 gatctgcgcg ctcgcgatgg gcgcggcggc gatggccgg
//
LOCUS       DATLJT010001450         2957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3577426,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001450 DATLJT010000000
VERSION     DATLJT010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577426"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VM910_33340"
     CDS             complement(<1..382)
                     /locus_tag="VM910_33340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI67427.1"
                     /translation="MSHKSAPPTVSTIGIDLGKNSFHLVGLDQRGAIVWQLKFSRAQL
                     ERRLANIPCCLIGMEACSGAHYIARQLAALGHDVCLMPAQYVKPFLKGHKNDYRDAEA
                     IAEAVQRPTMHFVAIKTPEQMDSAG"
     gene            complement(535..963)
                     /locus_tag="VM910_33345"
     CDS             complement(535..963)
                     /locus_tag="VM910_33345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67428.1"
                     /translation="MQLFNAYESGVGVDLGYQDFATELATLPGKYATPAGELLLARDS
                     RGKPLGCVGLRPIKPHGCCEMKRRYVSPKARGLALGRALIAAIIEEAVRIGYNEMRLD
                     TLPTMGAAISVYKQVGFVPIEPYYETPIAGTLFFARPLAT"
     gene            complement(1152..1724)
                     /locus_tag="VM910_33350"
     CDS             complement(1152..1724)
                     /locus_tag="VM910_33350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HVI67429.1"
                     /translation="MPSQLSMFADESARPEGLHYRPDFISAQEEAALIGHIRVLPLEP
                     FQFGIYEGKRRVVFFGSRYDFTHQRLEAAEELPAWLVPLAGRVERFAALPSGSIKHAL
                     CTEYDVGAGIGWHRDKKHFDQVFGLSLASACKFRFRRKARNKWERFTLNAQPRSLYVM
                     TGASRHVWEHSIPPVESPRYSITFRTMAQA"
     gene            1901..2536
                     /locus_tag="VM910_33355"
     CDS             1901..2536
                     /locus_tag="VM910_33355"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVI67430.1"
                     /translation="MCNRIRGLKEWSEIPRSLTRSLINFEYNPNVAPTEQVPAFLAER
                     GKPLATRMARFGINLASSGGKRRPPLLNARTDSLRRGSFKIMLANKRCVIPAEGFYEW
                     REEHGKKQPYFFARKDGKPVMFAGVWDYSDVKGEAVPSFAILTDEPNGLVAPYHDRMP
                     VVLSDVERWLNLDTTLDDVDPLGPERFTVRAVNPAVNKVSEKNIDAIERAA"
     gene            complement(2722..>2957)
                     /locus_tag="VM910_33360"
     CDS             complement(2722..>2957)
                     /locus_tag="VM910_33360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67431.1"
                     /translation="TGLPPTLIQVGSDEILLDDSVRMAERMRAAGCQVELEIWPRMPH
                     TWQVWARVLPEARAAIERIGAFVQSKVEPQAVR"
BASE COUNT          571 a          925 c          924 g          537 t
ORIGIN      
        1 agcctgccga gtccatctgc tccggcgtct tgatcgccac gaagtgcatg gttggtcgct
       61 gcacggcttc ggcaatcgcc tcggcatcgc ggtagtcgtt cttgtgaccc ttgaggaacg
      121 gcttcacgta ttgcgcaggc atcaaacaaa cgtcgtgccc aagcgcggcc agctggcgag
      181 cgatgtaatg cgctccggaa catgcttcca tcccgatcaa gcagcacgga atgttggcga
      241 gccggcgctc aagttgagcg cgtgagaatt tgagttgcca aacgatcgcc ccgcgctgat
      301 cgagaccgac aagatggaag ctattcttac cgagatcgat gccgatcgtg ctgacagtgg
      361 gtggtgcgga cttgtgtgac atgggacgtg ctccttgtgc ctcttaatcc cccgcaagac
      421 cttacgtctt gcagggcggg agcacggccg ggccatccca ttagcggaca ttgcctcaga
      481 tgcgctgaag accggtaagt gccaatttgc ggcgtgggat gcgcgtccgc cggatcaggt
      541 tgcgagtggt ctcgcgaaga ataacgtgcc cgcgataggt gtttcgtagt agggctctat
      601 cggtacgaac cccacttgct tgtaaacgga gatcgccgcg cccatggtcg gcagagtatc
      661 cagccgcatt tcgttgtacc cgatccgcac cgcttcctcg atgattgcag cgatcagcgc
      721 cctgccgaga gcaaggccac gcgccttagg tgagacgtag cgccgcttca tctcacagca
      781 cccgtgaggc ttgatcggcc taaggcctac gcatcccaac ggctttccac ggctgtcgcg
      841 cgcaaggagc aattcaccag ccggcgtcgc gtatttgccg ggtagggtcg ccagttccgt
      901 ggcaaaatcc tgatagccaa gatcgacccc aactccggat tcgtatgcat tgaacaactg
      961 cactgtggct tcaagatcag cggccgatct gactggtttt atttggaaca aggtcacctc
     1021 gtcggcaggc cgcaggtcat gaatcgaggc ggggcgctgt cggcgtctcc ggcgcttgca
     1081 acggaaaggg tagcacacct cgatgtggca ggagactgct gcgctgcggg atttcaagtc
     1141 gaatccgatg ctcaggcttg ggccatcgta cggaaggtga tcgaatagcg cgggctctcg
     1201 accggcggga tgctgtgctc ccagacatgg cgggaggcgc cggtcatcac gtagagcgag
     1261 cgtggctgcg cgttgagcgt gaaacgttcc cacttgttgc gggctttgcg gcggaaacgg
     1321 aatttgcagg ccgaagcgag ggaaagcccg aagacctgat cgaagtgctt tttgtcgcga
     1381 tgccagccga tgccggcgcc gacatcatac tccgtgcaga gcgcatgctt gatgcttccc
     1441 gaaggcaagg cggcgaagcg ttcaacccgt ccggccagcg gcaccaacca ggccggcagc
     1501 tcctcggccg cttccagccg ttgatgcgtg aagtcgtagc gcgagccgaa aaacaccacg
     1561 cgccgcttgc cttcgtagat gccgaactgg aacggctcca agggaagaac gcggatatgg
     1621 ccgatgagcg ctgcttcttc ctgcgcgctg atgaagtcag gccggtagtg cagcccctcg
     1681 ggccgcgcgc tttcatcggc gaacatgctt agctgggaag gcaaggggag gagcgctcct
     1741 tcaggctgat cccgacccgc gcagcaaaag cgcgcgcttc aacggcgtcg cgcgggcagg
     1801 ggtcgcgcac actattccaa ggctgccgca gcgcccatat atgtctttaa gttccgccgc
     1861 gcccatgccg cgaggggaga cagagtcagc catacctgcc atgtgcaacc gtatccgcgg
     1921 cctcaaagag tggagcgaaa tcccgcgcag cctcacgcgc tcgctcatca acttcgaata
     1981 caatcctaac gtcgcgccga ccgagcaggt gccagcgttc ctcgccgagc gcggcaagcc
     2041 gctggcaacc aggatggcgc ggttcgggat caacctggcc agcagcggcg gcaagagacg
     2101 cccacccttg ctcaacgccc gcaccgactc gctgcggcgc ggctcgttca agatcatgct
     2161 cgcgaataag cgttgcgtga tcccggccga gggcttctat gagtggcgcg aagagcacgg
     2221 caaaaagcag ccttatttct tcgcgcgcaa ggacggcaag cccgtcatgt ttgccggcgt
     2281 ctgggattat tcggacgtca aaggtgaagc ggttccgtcc ttcgcgatcc tgaccgacga
     2341 gccgaatggg ctggtcgcgc cctatcacga ccgcatgccc gtggtgctca gcgacgtgga
     2401 acggtggctt aacctcgaca ccacgctcga tgatgtcgac ccgctgggac ccgagcgttt
     2461 caccgtgcgc gcggtcaatc cggcggtcaa taaggtctcc gaaaagaaca tcgacgcgat
     2521 cgagcgcgcc gcctgaaccc ccgagcctca aaagccgcac gagccgctcg ccatgtgcaa
     2581 tcctgctctg gtgggcacat gggaccggct cacgcccgga gtcagaacga ttcaggtctg
     2641 acccaaggac gtgccagcct ggccgtggca ggctgagcgt gaaaccgacc tgcgccccac
     2701 caggccaggg gcgcgaccgt tctagcgcac cgcttgcggc tctaccttgc tctgcacgaa
     2761 ggcgccgatg cgctcgatgg ccgctcgcgc ttccggaagg acccgcgccc agacctgcca
     2821 ggtgtgcggc atccgcggcc aaatttcgag ttcgacctga catccggcgg cgcgcatccg
     2881 ctccgccatg cgcacggaat catcgaggag gatctcgtcg ctgccgactt ggatgagcgt
     2941 cggcggcagt ccggtcg
//
LOCUS       DATLJT010001451         3491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_591050,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001451 DATLJT010000000
VERSION     DATLJT010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591050"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..693)
                     /gene="upp"
                     /locus_tag="VM910_33365"
     CDS             complement(58..693)
                     /gene="upp"
                     /locus_tag="VM910_33365"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HVI67432.1"
                     /translation="MATANVHLVNHPLVQHKLSLMRERDRSIKSFRELLNEIGMLLCY
                     EVTRDLPLELVDIETPLAPMRAPKIAGKKLTFAPILRAGVGFLDGMLALVPSARVAHI
                     GLYRDPETLEAVEYYFKAPQDLSDRTVIMMDPMLATGNSACAAAKLLKARGARDIRFV
                     CLLAAPEGIAQFQKLHADVPVWTAAVDERLNDHGYILPGLGDAGDRMFGTK"
     gene            complement(797..>3491)
                     /locus_tag="VM910_33370"
     CDS             complement(797..>3491)
                     /locus_tag="VM910_33370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI67433.1"
                     /translation="LRANPSTPRTLIDKGNVDAAIGSAARPMQRTYVWPYQMHASIGP
                     SCAVADYGDGQIRVWSGTQNPHLLRSDLALLMERPEAEIEVIRLEAAGCYGRNCADDV
                     TADALLLSRAVGRPVRVQLTREQEHAWEPKGAAQLMEVNGGLNADGTVAAYDFATRYP
                     SNGAPTLALLLTGRVAPVPEVFEMGDRTAIPPYDYENLRVVVNDTPPIVRAAWLRGVS
                     ALPNTFAHESYIDELATDADVDPIEYRLRYLKDKRAIDLVNAVAERAGWKPRPIREMK
                     AAEDDVLRGRGFAYALYVHSKFPGYGAAWSAWIADVAVNTKTGGVSVTRVVAGQDSGL
                     MINPDGVRHQIEGNVIQSTSRALMEEVAFDRTSVTTREWGAYPIIGFPDLPDIDVLML
                     PRQDQPPLGVGESASVPSAAAIANAIFDATGVRFREPPFTPERILKGLHGEDVAASGA
                     LPAPASTLAPTRDSWSPFARRRGAFASMVALCTAAIGVGMALLPWRAMAPIARPDASV
                     FSAATIARGAQLAALGNCAVCHTNARGAVNAGGRPIETPFGVIYSTNITPDVETGIGA
                     WSYPAFERAMREGIHRDGRRLYPAFPYTHFARASDADLQALYAYLIAQPSARAKAPAN
                     ELRFPFNFRPLLAGWNALFHGDNSFKPDPARSDVWNRGAYLVEALGHCSACHSPRNAL
                     AAEVSNAYLAGGFAEGWEAPPLASLSLAPIPWDEDELYAYLRTGESRFHGTAAGPMAP
                     IVRDLAALPDEDVRAMAVYLASFGDETKESTKQALAKQLEDSTRTTLASSTGARLYQG
                     ACAVCHEVGGLPLFGSRPSLALNSNLHSHVPDNLIQVILHGIAEPPSSDLGYMPAFKD
                     SMNDMQIAELVSYLRRQFAPDKPAWSDVVSAVSRIRRSRVN"
BASE COUNT          623 a         1062 c         1175 g          631 t
ORIGIN      
        1 gcatttttta ttgatagctt cgatcgcaat aaatgatgcg gcgcgcgggc gccgcggcta
       61 ctttgtgccg aacatccggt cgccggcgtc gccgaggccc ggcaggatat agccgtgatc
      121 gttcagccgc tcgtcgaccg ccgccgtcca gaccggcacg tcggcatgca gcttctggaa
      181 ctgcgcaatg ccttcagggg ctgcaagcag gcagacgaac ctgatatcgc gggcgccgcg
      241 tgctttcaga agcttcgctg cggcgcaggc ggaatttccg gtcgcgagca tcgggtccat
      301 catgatcacc gttcgatccg acagatcctg cggcgccttg aaatagtatt cgacggcttc
      361 cagggtttcg gggtcgcggt aaaggccgat atgggcgacc cgcgcggatg gtaccagcgc
      421 cagcatgccg tcgagaaagc cgacgcctgc gcgcaggatc ggcgcaaagg tcagcttttt
      481 gccggcgatc ttgggcgccc gcattggcgc aagcggcgtc tcgatgtcga ccagttcgag
      541 cggcaggtct cgcgtcactt cgtagcagag cagcatgccg atctcgttga gaagctcgcg
      601 aaagcttttg atcgagcgat cccgttcgcg catcagcgag agcttgtgct gaaccagcgg
      661 gtggttgacg agatgaacgt tagcggttgc catgcggagc gttatccttc gagcgttccc
      721 ggaggttttc tctacaccag ttttgaagca aactggagtg ttggcgggga cgaaacgagg
      781 cgatttgggc gcctgatcag ttgacgcgcg atcgacggat ccggctgacc gcagaaacca
      841 catcgctcca ggccggcttg tcgggcgcga attgccgccg cagataagag acgagttcgg
      901 cgatctgcat gtcgttcatg ctgtccttga aagccggcat gtagccgaga tcgctcgacg
      961 gcggttcagc gatcccgtgc agtatcacct ggatgagatt gtcgggcacg tggctgtgca
     1021 ggttgctgtt cagcgcaagc gatggccggc tgccgaacag cggtaggccg cccacttcat
     1081 ggcagaccgc acaggcgcct tgatagagcc gcgcgccggt cgacgaagcg agtgtcgtgc
     1141 gcgtgctgtc ttcgagttgt tttgccagcg cctgctttgt ggactccttg gtctcgtcgc
     1201 cgaaggaagc gagataaacc gccatcgcgc gaacatcctc gtcgggcagc gccgcgagat
     1261 ccctgacgat cggtgccatc ggtccggcgg ctgtgccgtg aaagcgggac tcgccggtgc
     1321 gcaagtaggc atagagctcg tcttcgtccc aagggatcgg tgccagcgaa agcgaggcga
     1381 gaggaggggc ttcccagcct tcggcgaagc cgccggcgag atacgcgttc gatacctccg
     1441 cggccagcgc attgcgcggc gagtggcacg cgctgcaatg accgagagcc tcgacgagat
     1501 aggcaccgcg attccagacg tcggatcttg ccggatcggg tttgaacgaa ttgtcgccat
     1561 ggaacagcgc gttccagccg gcgagcagcg gacgaaaatt gaacggaaat cggagctcgt
     1621 ttgccggcgc tttggcgcgc gcggaaggct gagctatgag gtaggcgtaa agcgcctgca
     1681 gatcggcatc gctcgctctt gcaaaatgcg tgtagggaaa cgccggataa aggcgtcgac
     1741 cgtcccggtg aatgccttcg cgcatcgcgc gctcgaaggc cggataggac caggcgccaa
     1801 tcccggtttc gacgtcgggg gtgatgttgg tgctgtagat gaccccgaat ggcgtctcga
     1861 tcgggcggcc tccggcattt accgcgccgc gggcgttggt gtggcagacc gcacaattgc
     1921 cgagtgcggc aagttgggcg ccgcgcgcaa tcgtcgcggc actgaagacc gaggcatccg
     1981 gccgcgcgat cggcgccatg gcgcgccacg gcaacagcgc catgccgacg ccgatcgcgg
     2041 ccgtacacag cgcgaccatg ctggcgaacg cgccacgccg cctggcaaac ggactccagc
     2101 tatcccgcgt cggcgctagc gtagatgcag gtgcgggcag ggcgcccgaa gccgcgacgt
     2161 cttcgccatg cagccccttc agaatacgtt ctggcgtgaa cggcggttcg cggaagcgca
     2221 cgcccgtggc gtcgaaaatg gcgttggcaa tcgcggccgc gctcggcacc gatgccgatt
     2281 cgccgacgcc aagcggcggc tggtcctgcc gcggcagcat caggacgtcg atatcaggca
     2341 gatcgggaaa gccgatgatg gggtaggcgc cccattcacg cgttgtcacg gaggttcggt
     2401 cgaacgcaac ttcctccatc agcgcgcggc tggtggattg gatgacgtta ccctcgatct
     2461 ggtgccgtac gccatccggg ttgatcatca gcccggagtc ctggcccgcg acgacgcgcg
     2521 tgacgctgac accgcccgtc tttgtgttga cagcgacgtc ggcgatccag gccgaccatg
     2581 ccgcaccgta acccggaaat ttgctgtgga cgtaaagcgc ataggcaaag ccgcgaccgc
     2641 gcaggacgtc gtcttcggca gctttcattt cgcgaatcgg acgtggtttc cagccggcac
     2701 gctcggcgac ggcgttgacg aggtcgatcg cgcgcttgtc cttcagatag cgcagccgat
     2761 attcgatcgg gtcgacgtcg gcgtcggttg caagctcgtc gatataggat tcatgcgcaa
     2821 aggtattcgg cagcgccgag acgccgcgca gccaggccgc gcgcacgatc ggtggcgtgt
     2881 cgttgacgac gacgcgcagg ttttcgtagt cgtagggcgg gatcgcggtg cgatcgccca
     2941 tctcgaagac ttcggggaca ggcgcgaccc ggccggtgag gagcagcgcc agcgtcggtg
     3001 cgccgtttga gggatagcgt gtggcgaaat cgtaagcggc aaccgtgccg tccgcgttca
     3061 gcccgccatt gacctccatc agttgcgcgg cgcctttcgg ttcccaggcg tgctcctgct
     3121 cgcgcgtcaa ctgcacgcga acgggacgac cgacggcacg cgacaacagg agcgcatcgg
     3181 cagtcacgtc gtcggcgcag ttgcggccat agcagccggc agcttccagt cggatcacct
     3241 cgatctcggc ctccggacgc tccatcagga gggcgaggtc gctgcgcaac agatgcggat
     3301 tttgtgtgcc ggaccacacc ctgatctgac cgtccccata gtcggcgacc gcgcaggagg
     3361 gaccaatcga ggcgtgcatc tggtaaggcc agacgtaggt gcgttgcatc ggccttgccg
     3421 cacttccgat cgcggcatcc acattgccct tgtcgatcag ggttcgcggc gtcgacggat
     3481 tggcgcgcag c
//
LOCUS       DATLJT010001452         1601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3118328,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001452 DATLJT010000000
VERSION     DATLJT010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118328"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(516..1250)
                     /locus_tag="VM910_33375"
     CDS             complement(516..1250)
                     /locus_tag="VM910_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67434.1"
                     /translation="MFSIAPLTSLSAMTPPVDPDPIASFRDLVAVPPDTAREAPNPRR
                     MRTLVDRGVVEYASVKTDGDRAKGAGLIQTAALVGYPPARALLARNYPQSEAVRSVVP
                     AEDVIRYALGPVMDAAATGEDSKQIFLALGQHFALQGQVDLFATQILSSLRGDSRPQL
                     GYRIDTLLDLLARVPGACGAVARLIPGEREAADRECAFGQNLRKHIETTAPAREEEES
                     KRRGLLMLSHPSGRWRGVVRGWSAAS"
BASE COUNT          324 a          500 c          504 g          273 t
ORIGIN      
        1 ttggatttcg gcagtcgcac tgtgacattc aatgatccac tgttagggcc ggccaacatc
       61 agtgtcagtg agcgcatcag cgaggtgaag ctcggcgtca actatcgctt tggctccccg
      121 cttccgacat attgatcacc aggtcgggcc gctcttgccg gccggttgcg gcgactttca
      181 aggggagcgt cgcgagcggt gtgaagtggt cgcagcgttt gcgcgcgacg agcagcgcag
      241 cggcccaaaa ggtcggcgac aggcggccct aagctcgcga tgaacgttac tggctgctgg
      301 cgcggtcagc cgaaaggccg cagcgtaatg tcagcttgct tgcatcgtcc accaccaccc
      361 atatggacca gcggcgtggg ccaaagtagt tgatgctcac gggaacgaag ttgcggcttc
      421 gccatcgcaa gtcgcagttt gccaaatgtc cttctcgaag aaatcttgca cgcaagacgg
      481 ctacgtagat tgaggcgcca cacgcctggc aatattcaac ttgcagccga ccagccccga
      541 acgacgccgc gccagcgtcc gctggggtga ctcaacatga gcaaaccgcg gcgcttcgac
      601 tcctcctcct cacgggccgg cgccgtcgtt tcgatgtgct tgcgcagatt ttggccgaaa
      661 gcacattcgc ggtcggcagc ctcgcgctcc cccggtatca gccgggcgac cgcgccgcag
      721 gctccgggca cgcgcgccag caagtccagc aaagtgtcga tccgataacc gagctgcgga
      781 cgcgagtcgc cgcgtagtga actgaggatc tgggtggcga acagatccac ctgcccttga
      841 agcgcgaaat gttgtccgag cgcgaggaat atttgcttcg aatcctcgcc ggttgccgcc
      901 gcatccatca cggggcccag cgcatagcgg atgacatcct cggcaggaac gaccgaacgc
      961 accgcttcgg attgcggata gttgcgcgcg agcaaggcgc gcgccggagg gtagcccacc
     1021 aaggcggcag tttggatcaa gcccgcgcct ttggcccggt ccccatcggt tttgaccgac
     1081 gcgtattcga cgacgccgcg atcgaccagc gtgcgcatcc tgcggggatt gggcgcttca
     1141 cgcgcggtat cgggcggcac cgcaacgagg tcgcgaaagc tcgcaatcgg atcgggatcg
     1201 accggcggcg tcatcgccga caacgacgtc agcggcgcga tggagaacat gatcgcgctc
     1261 tgtgcgttgc tcaatccctc gctaccggag ggatcgatgt aagcgtctct tgggacctcg
     1321 atgacgatgt tgatgaccgg cccactgtaa ccgcttcggc cggattagcc gacgaaacat
     1381 gattgcgata tgacgcccag aatagggagg cgacgagcag gggagccaga atgacagcga
     1441 gacagctcgc agccgatggc aactttcgct cacccagcat cggaagctct ccgaaagcgg
     1501 cggcgacaaa gtcacgagcg aatcttcacc accaccagcc gggacgcaga tatggccggc
     1561 gatgaacccg ctgtacagcg tgaacggaca cgcgccggcg c
//
LOCUS       DATLJT010001453         1533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2428057,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001453 DATLJT010000000
VERSION     DATLJT010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428057"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VM910_33380"
     CDS             <1..138
                     /locus_tag="VM910_33380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HVI67435.1"
                     /translation="PHPRWSQATEELIGRNERRPTLIFNGYGDHVADLYRGLENERLW
                     A"
     gene            complement(258..>1533)
                     /locus_tag="VM910_33385"
     CDS             complement(258..>1533)
                     /locus_tag="VM910_33385"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI67436.1"
                     /translation="PGEAASVMTCIDGYAMAQKGRAGAALCIAAVGSWIAGTFGVIML
                     TLIAPPLASFALRFGPPEYTALLVLGLVFLAYMSSTSLPRTLLMACFGLLLGTIGIDN
                     MTGHFRYSFDIAELGDGIGIVPVAIGLFGLGEILSTPSANVISKVVAPKLRQLLPNRR
                     EWRQSAMPIARGSVLGFLIGLTPGSAHIISSFLSYAIERRVSKHPEEFGKGAVAGVAG
                     PESANNAASTGAFVPMLALGLPTGPITAVLIAALMIHGVPPGPTLVNEHPNIFWGFVA
                     SMYVGNLMLLALNLPLVGIFVNVLRIPYTYLYPLVIMFCIIGVYEVSHSIVDVWIMLI
                     MGIIGYVLRKFGFDPAPLVLGLVIAPTLEMSLRQSLIMSNGNWAIFLQRPISLTLLAV
                     CAALLALAALSLIRSRKDWRSQLAEAETGENQ"
BASE COUNT          314 a          469 c          520 g          230 t
ORIGIN      
        1 ccgcatccgc gctggagcca ggccacggaa gagctgatcg ggcgcaatga acgccggccg
       61 acgctcatct tcaacggcta cggcgaccat gtcgccgacc tttaccgcgg gctcgagaac
      121 gagcgcctgt gggcatgagc gtgcgcggcg ttgcgcgtgc cggccggaag aagcggattg
      181 atccggtagc aagtcctccc tgcgcgaggt gatcgttggt ggttttcccg gcgcgggatc
      241 gctgcgccgc gaacacgcta ctggttttcg ccggtttcgg cctcggccag ctgactgcgc
      301 cagtccttgc ggctgcggat gagcgacagc gcggcgagcg ccagtagcgc cgcacagacg
      361 gcgagcagcg tgagcgagat cgggcgctgg aggaagatcg cccagttgcc gttcgacatg
      421 atcagcgatt ggcgcagcga catctcgagg gtcggcgcga tcaccaagcc gagcaccagc
      481 ggcgccggat cgaagccgaa cttgcgcaag acgtagccga tgattcccat gatcagcatg
      541 atccacacat cgacgatcga atggctgacc tcgtagacgc cgatgatgca aaacatgatc
      601 accagcggat aaagataggt gtaggggatt cgcaatacgt tcacgaagat gccgaccagc
      661 ggcaaattga gcgcaagcaa catcagattg ccgacgtaca tcgatgcgac gaacccccag
      721 aagatgttgg gatgctcgtt caccaacgtc ggccccggcg gcacgccgtg gatcatcagc
      781 gcggcgatca gcacggcggt gatcggaccg gtaggaaggc cgagcgccag catcggcacg
      841 aaggcgccgg tcgaggccgc gttgttcgcc gattcgggtc cggcaacgcc ggcgactgca
      901 cccttgccga attcttccgg gtgcttggag acgcgtcgct cgattgcata ggacaggaag
      961 ctcgaaatga tgtgggcgga acccggggtg agcccgatca gaaagccgag aaccgaaccg
     1021 cgggcaatcg gcatggccga ttgccgccat tcccggcggt tcggcaacag ctggcgcagc
     1081 ttgggcgcga ccactttgct gatcacgttt gccgacggcg tcgacaggat ctcgccgagc
     1141 ccgaacagcc cgattgcgac cggaacgatg ccgatgccgt cgccgagctc ggcgatgtcg
     1201 aaggagtagc ggaagtggcc ggtcatgttg tcgatgccga tagtgccgag caggagcccg
     1261 aaacacgcca tcagcagcgt gcgtggcagc gaggtcgacg acatataggc gaggaagacg
     1321 aggccgagca cgagcagcgc ggtgtattcc ggcggcccga accgcaacgc gaaggaggcg
     1381 agcggcggcg caatcaatgt cagcatgatc accccgaacg tgccggcgat ccacgagccg
     1441 acggcggcga tgcacaacgc cgcgcccgcg cgcccttttt gcgccatggc gtagccgtcg
     1501 atgcaagtca tgaccgaggc ggcttcgccc gga
//
LOCUS       DATLJT010001454         3858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3118407,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001454 DATLJT010000000
VERSION     DATLJT010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3118407"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VM910_33390"
     CDS             <1..338
                     /locus_tag="VM910_33390"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HVI67437.1"
                     /translation="RGIDVRLIADKTTPCERNSAVGPLAAAGVPIWIDDQARIAHAKT
                     MVIDGAITLTGSYNWTRGAAANSENLNLISSPAVAAAYAAHWRQRLAVSIRFDRKEDW
                     CRVATTEAR"
     gene            complement(562..825)
                     /locus_tag="VM910_33395"
     CDS             complement(562..825)
                     /locus_tag="VM910_33395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67438.1"
                     /translation="MLGKHVTIVVGRMLILALIVATANTGRAAAASGVDPFQTSLNGS
                     MPPRTEGLNLSFTVPAGKTLVIEYVSGNCFVPAGQARVLSLIT"
     gene            complement(1230..1454)
                     /locus_tag="VM910_33400"
     CDS             complement(1230..1454)
                     /locus_tag="VM910_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67439.1"
                     /translation="MRALSDRVLSRTGPTQFPESLRGVPGDLIVALSDGDFISFGMRW
                     PSLEKALVAIKRDRFWPLSLPNVARKRDRS"
     gene            complement(1536..1790)
                     /locus_tag="VM910_33405"
     CDS             complement(1536..1790)
                     /locus_tag="VM910_33405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67440.1"
                     /translation="MNNARNAGLALWRRIRRFRPSRAMAGHLVLGLALLPPLPCQAQA
                     PAHSAAGGMVAPWMRVPAVVVVGANANDARLPLVEDAVAF"
     gene            complement(1858..2496)
                     /locus_tag="VM910_33410"
     CDS             complement(1858..2496)
                     /locus_tag="VM910_33410"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI67441.1"
                     /translation="MAHPIVYGPAFSSYVWSARLALAEKGVTHELVDVPFGAQREEPH
                     LSRQPFAKVPAFEHDGFVLYETQAIIRYVDERFAGTPLQPEDVHQWSRMNQIIGIVDA
                     YAWPSIAGAILFNRVLVPRFLGGTPDEAAIAAALPRARLCLAEIERLMEDHRFLAADF
                     VSLADLMVIPLLYYFGNVPDGRAPIAEHPKLQSWMRHMETRQSFQVTKPPPL"
     gene            complement(2512..>3858)
                     /locus_tag="VM910_33415"
     CDS             complement(2512..>3858)
                     /locus_tag="VM910_33415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI67442.1"
                     /translation="RERMKEILDQLKRRGVFTVVGGPWVTVNEDDFAGLADVIFIGEA
                     EQTWPRFLNEWGEGRHRIRYEQAERTDMATVPVPRLDLLRMRDYAFGSVQFSRGCPFE
                     CEFCDIIVTFGRRPRIKSSAQVLAELDALVAAGKPAAFVVDDNLIGNKKAIKPILRDI
                     VAWQEQHGFPVTLVTEASIDLADDAELMELMVEANFVSVFVGIETPNEASLRETKKLQ
                     NLRSRGGTMLEKVHRLQDAGIEVWCGIIVGFDNDEAGIFAAQNRFVRASRIVNAMVNM
                     LVAIPRTPLYNRLAGEGRLDLSEEWAGYGGFGTNVVPLRIGREALYAGYIGLMRELYS
                     TDAYFGRLDELFFSGRFQLSKARKRYLRRHPINWFKLNVRLFFEAVGMFVMLMRGVPD
                     RALRREYRRRLLRAAWRSWDADMIWNYALKCAMHYHIDRIVQELLSRSQPSTRLAA"
BASE COUNT          777 a         1206 c         1185 g          690 t
ORIGIN      
        1 agcgcggcat cgacgttcgg ctgatcgccg acaagacaac accctgcgag cgcaacagtg
       61 ctgtgggacc gctcgccgcc gctggcgtgc cgatctggat cgatgatcaa gctcggattg
      121 cgcacgccaa gaccatggtc atcgacggtg ccatcaccct gacgggctcc tacaactgga
      181 cccgtggcgc cgcagcgaac tcggagaacc tgaacctgat ctcctcccct gccgtcgcgg
      241 cagcctatgc cgcgcattgg cggcagcgtc ttgcggtttc gatccggttc gacaggaaag
      301 aggattggtg ccgggtcgcg acgacggaag cgcgttgaca ggcgatgaca aaccctattg
      361 tagatgacct gaaaggctca tcacgatggg cgtggcggag ccggttgcag agttgcgatc
      421 ggctctgagc gtcacggtct taccgccatc ggcgtagagc agtacctgct ggccgaccct
      481 ccacagcaca tttccgccgc cgaaggctcc gacgccggcg gttggcaggt tgaactgagt
      541 cccggtcgtg gcgccgttga cttaggtaat gagcgagaga acccgcgcct gaccggccgg
      601 cacaaagcag ttgccggaga cgtattcgat gaccagcgtc ttgccggccg gtactgtgaa
      661 cgagaggttc agcccttctg tcctgggcgg catcgagccg ttcaaacttg tttgaaacgg
      721 atcgacgccg gaagcggcag ccgcgcgccc tgtgttggcc gtcgcaacga tcagcgccaa
      781 aattagcatt cgacctacaa cgatggtgac atgtttaccg agcatgtgtc gtttcctttt
      841 cctcaagcgg gaagttgctc tggagcagca tagaactggc ttgcctcgaa atgtgtgagc
      901 ctactcacac tctgcctttg ctcgcgaagt acgggtggtc ccggcgaagt accgagagct
      961 cgggacgggc tgtccactgc ggttttgcaa gaaattcacc cgctcagcgt cagcgggcgc
     1021 accagcgtta gcaaagcgca aatggggagg gactaccgag cccccaatcg gcggggtaaa
     1081 ggctcagcag cagcgctttt tccgctgcgg tcagcggaaa ataacgctcg gttggagaag
     1141 cgaaagcatc cggccggcac ggagcaggac ggccgcacat caatgccgtt gcatcgctgt
     1201 tgtggccaag gccgatcgca tgcccgattt catgagcgat cacgtttgcg ggcaacgttg
     1261 ggcaagctga gcggccagaa ccggtccctc ttgatcgcca caagggcctt ctcgaggctg
     1321 ggccagcgca taccaaacga gatgaaatcg ccgtccgaca gcgccacgat cagatcgccc
     1381 gggacgccgc gcaggctctc cgggaactgc gtcgggccgg tgcggctcaa aacccggtcg
     1441 ctcagcgcgc gcaactcgtc ggctggggtc gccgcagtgg cttgggttaa cggccccagt
     1501 cggaaggcgg agccgagttc ggcgaaactg cggttctaga acgcaaccgc gtcctccacc
     1561 agcggcagcc gcgcatcatt cgcgttcgcg ccgacgacaa ccaccgccgg aaccctcatc
     1621 cagggcgcga ccatgccgcc ggcagcagaa tgggctggcg cctgcgcctg acacggcaga
     1681 ggcggcagca gcgccaaacc caggaccaaa tgcccggcca tcgcccgact tggtcgaaaa
     1741 cggcggatac gacgccacag cgccaggcca gcattgcggg cgttgttcat ggcgctcgaa
     1801 gagcgggtca aaagcgccgt cgcgccgtac cgttcgtcgc ggcgcccgga caccttgtta
     1861 cagcggcggc ggcttcgtca cctggaagct ctgccgcgtt tccatgtggc gcatccagct
     1921 ctgcaatttc gggtgttcgg cgatcggcgc ccggccgtcc ggcacgttgc cgaaataata
     1981 gagcagcggg atcaccatca gatcggcgag gctgacgaaa tcggccgcga gaaaccggtg
     2041 atcctccatc agccgttcga tctcggcgag gcagaggcgg gcgcgcggca gcgccgcagc
     2101 gatcgccgcc tcatccgggg tgccgccgag aaagcgcggc accagcacgc gattgaatag
     2161 gatcgcgccg gcaatgctgg gccaggcata ggcgtcgacg atgccgatga tctggttcat
     2221 tcgcgaccat tggtgcacgt cctccggctg caacggcgtc cccgcgaagc gctcgtcgac
     2281 atagcgtatg atcgcttggg tttcgtagag cacaaacccg tcatgttcga aggcgggaac
     2341 cttagcgaaa ggctggcgcg acaggtgcgg ctcctcacgc tgggcgccga acggcacgtc
     2401 gacaagctcg tgcgtcacac ctttttccgc cagcgcgagc cgggcactcc acacatagct
     2461 gctgaaggcc gggccgtaga cgatcggatg agccattgtt tctccggtga ctcaagcggc
     2521 gaggcgggtc gaaggctgtg accgagagag cagctcttgg acgatcctat cgatgtgata
     2581 gtgcatcgcg catttaaggg cgtaattcca gatcatgtct gcatcccaac tgcgccacgc
     2641 cgctcgtagc aagcggcggc gatattcgcg gcgcaatgcc cgatcgggca cgcctcgcat
     2701 caacatcacg aacattccga ccgcttcgaa aaaaagccgt acattgagct tgaaccaatt
     2761 gatcgggtga cggcgcaaat atcgtttgcg tgcttttgac agctgaaagc gtccactgaa
     2821 gaataactcg tctagccgtc caaaataggc gtcggttgag taaagctcgc gcatcagccc
     2881 gatatagccg gcgtagagcg cctcgcgacc aatcctaagc ggcacaacat ttgtgccgaa
     2941 gcctccatag cccgcccact cctccgaaag gtcgagcctg ccttcgcctg caagacgatt
     3001 gtagagggga gttctcggta tcgccacgag catgttgacc atcgcattaa cgatccgcga
     3061 cgcgcgcaca aagcgattct gggcggcaaa aatgcccgcc tcatcattgt cgaacccgac
     3121 aatgatgcca caccagactt ctattcccgc atcttggagc cggtgcacct tttcgagcat
     3181 ggtgccaccg cggctgcgca ggttttgcag cttcttggtc tcacgcagcg aggcttcatt
     3241 gggagtctcg atcccaacga agacgctgac gaagttcgcc tcgaccatca gctccatcag
     3301 ctccgcgtcg tcggccagat cgatcgaggc ttcagtcacc aatgtcaccg gaaagccgtg
     3361 ctgttcttgc caagcgacga tatcgcgcag tatcggcttg atcgccttct tgttgccgat
     3421 cagattgtcg tcgacgacaa aggcagcagg cttgcccgcg gcaacgaggg cgtcgagttc
     3481 ggcgagtacc tgggccgaac tcttgatccg cggacggcgg ccgaaagtca cgatgatgtc
     3541 gcagaactcg cactcgaaag ggcagccgcg cgagaactga acgctgccga atgcgtagtc
     3601 gcgcattcgc agcagatcga gacgcgggac cggaacggtc gccatgtcgg tccgctcggc
     3661 ctgctcgtag cggattcgat ggcgtccctc gccccactcg ttgaggaacc gtggccaagt
     3721 ctgctcggcc tcgccaataa atatgacatc ggcgagaccg gcgaagtcgt cctcgtttac
     3781 cgtaacccat gggccgccga cgaccgtgaa gacgccgcgg cgcttgagct gatcgaggat
     3841 ttccttcatc cgctcgcg
//
LOCUS       DATLJT010001455         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_817063,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001455 DATLJT010000000
VERSION     DATLJT010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817063"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VM910_33420"
     CDS             <1..462
                     /locus_tag="VM910_33420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67443.1"
                     /translation="RGAGRILDDMGVSGAVQFSGSGPIRRVRLPGNTLQIRGQAVCAS
                     LRGMPFEPCFNLEKKDERSFRGSVSGMSFAYCDFRHQGAAQMLMARAVARPRSMQRIE
                     QTGPTDVPHAEIAVRVETPPVESSKIAPVKSEPQAAPVKSESAPELRRSTE"
     gene            591..824
                     /locus_tag="VM910_33425"
     CDS             591..824
                     /locus_tag="VM910_33425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67444.1"
                     /translation="MPLYYFRISHGRYCGASDQPYEFENREAAWTEMTALCANLVGGI
                     SRSLTQNAEWHMELLDETKKPVFRIRLVGESLD"
     gene            complement(841..>2091)
                     /locus_tag="VM910_33430"
     CDS             complement(841..>2091)
                     /locus_tag="VM910_33430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HVI67445.1"
                     /translation="ALYARIAHLDRSIPFAILAVVLAAAFGGATEVLARRDSRPGLMI
                     STALFATGTLGALALALTFALEKGWLTIALALMSMGTSWISLQRPIPFLRTLAAVLAG
                     IVVLRVGYELRIAGDAVGDTPIFNWLLWGYGIPAASFWIGSYFLRRRGDDAPLRAVEA
                     AAILFTVLLAFLEIRHAIYGGGRFYRAVPQLSEFAADVCVALALAIGLERLRVRTGSI
                     VHNIGAILLTVFAGLVGVFGLLILENPTITRMDISGQFVNLLLLAYALPAVLMLLLSY
                     AVAGRRSTLYANTIAAGALVFALAYVTLEIRRLYHGPNMIEGLTGDAEQYTYSIAWLT
                     FGVVLLGIGLLVNSERARLASAAVIALTILKAFVIDMSALTGVYRALSFMGLGLVLVA
                     IGWLYQKILFRRRAPPPVPASPNS"
BASE COUNT          422 a          654 c          694 g          321 t
ORIGIN      
        1 cgaggcgccg gccgcatcct cgacgacatg ggagtttcgg gcgcagtgca gttctcgggc
       61 tcgggcccga tccgtcgcgt tcgcctgccg ggtaacacgc tgcagatccg cggtcaggcg
      121 gtgtgcgcct cactgcgtgg aatgccgttc gaaccctgct tcaatctcga gaagaaagat
      181 gagcgcagct tccgcggctc ggtttccggc atgagcttcg cttattgcga tttccgccac
      241 cagggcgcag cacagatgtt gatggcgcgc gcggtggcgc ggccccgctc gatgcagcgt
      301 atcgaacaga ccggcccgac ggacgtgccg cacgccgaga tcgccgtgcg ggtcgagacg
      361 ccgccggtcg aaagctccaa gatcgcaccg gtgaagtccg agccacaggc ggcgccggtg
      421 aagtccgaga gcgcgcccga attgcgccgc tcgacagagt gacctggcgg acgctccagc
      481 ggtaatatcg cgccgcatgc cgtagtctta cggatgctca agttcatgcg ttggtcgtac
      541 tcccgctcca gtgtgggacg agggggtagg tccactgcat ggtttagaga atgccgctgt
      601 attacttccg gatcagccat ggtcgctatt gcggcgcatc cgaccagcca tatgagttcg
      661 agaaccgcga ggccgcctgg accgagatga ccgcgctctg cgctaatctg gttggcggca
      721 tttccaggag cctgacgcaa aacgccgagt ggcacatgga gctcctcgac gaaaccaaga
      781 agccggtgtt tcgcatccgc cttgtcgggg agtcgttaga ctagcgggcg cagggtgccc
      841 tcatgagttc ggtgacgcgg gcaccggcgg cggtgcgcgc cggcggaaca ggattttctg
      901 atacagccag ccgatcgcga ccagcaccag accgaggccc atgaaggaca gggcgcgata
      961 gacgcccgtc agcgccgaca tgtcgatgac aaacgctttg aggatggtca gcgcgatcac
     1021 agccgcggag gcgagccgtg cgcgctctga attgaccaac aggccgatgc caagcaacac
     1081 cacaccgaaa gtcagccagg caatcgaata ggtatattgc tcggcgtcgc cggtcaggcc
     1141 ctcgatcatg ttcggaccgt gatagagccg gcggatttcc agcgtcacat aggcgagcgc
     1201 gaagaccagc gcgccggctg caatggtgtt ggcatagagg gtgctgcgtc ggccggccac
     1261 ggcataggac aacaacagca tcagcacggc cggcagcgca taggcgagca acagcaaatt
     1321 gacgaattgg ccgctgatgt ccatccgggt gatcgtggga ttttcgagga tcaacagccc
     1381 gaatacgccg accagcccgg caaagaccgt gagcaggatc gcgccaatat tgtgcacgat
     1441 gctgccggtc ctgacgcgca gccgctccag cccgattgca agcgcgagcg ccacacagac
     1501 gtcggccgcg aattccgaaa gctgcggcac ggcgcggtag aatcgtccgc cgccgtaaat
     1561 cgcatggcgg atttccagga atgccagcag cacggtgaac aggattgcgg ctgcctcaac
     1621 cgcgcgcagc ggtgcgtcat cgccgcggcg gcgcaggaag tagctgccga tccagaacga
     1681 tgcagcggga atgccgtaac cccacaacag ccagttgaag atcggcgtgt cgcccaccgc
     1741 atcgcccgcg atccgcagct catagccgac gcgcaggacg acgatgccgg cgaggactgc
     1801 cgcaagcgtg cgcaggaacg ggatcggtcg ttgcagcgaa atccagctcg tgcccatcga
     1861 catcaacgcg agtgcgatgg tcagccagcc cttctcgagc gcaaacgtca gggccagcgc
     1921 cagcgcgccg agcgtgccgg tcgcgaacaa agcggtcgag atcatcaggc ctggccggct
     1981 gtcgcgccgg gcgagcacct ccgttgcgcc gccgaaagcg gctgcgagca ccacggcgag
     2041 gatcgcaaac gggatcgacc ggtcgagatg ggcgatgcgg gcgtagagcg c
//
LOCUS       DATLJT010001456         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1969142,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001456 DATLJT010000000
VERSION     DATLJT010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969142"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..438
                     /locus_tag="VM910_33435"
     CDS             220..438
                     /locus_tag="VM910_33435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67446.1"
                     /translation="MASKQDQEKRRKLPRKERVLDKCGSTPGRGMEMPTRPAGLDPIV
                     AEIIEALARTIIRDEDRRTTLLFMNKNE"
     gene            1669..2088
                     /locus_tag="VM910_33440"
     CDS             1669..2088
                     /locus_tag="VM910_33440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67447.1"
                     /translation="MIRGTISEAEGDRIIPELRRQRDRAKAALASAAKPPKVIELHPP
                     SVDEYLRAIERLADYANGQLAKSDDELAQSIRRFIDSVTVMPAGSGDEPEIKVAGGLA
                     SLLPPESRGSGLPGEAVAGARYSRCKSPVDGAFVFAA"
BASE COUNT          569 a          712 c          762 g          456 t
ORIGIN      
        1 ggtgacgcat acgcccgtgc ccacctgatg catctaaatg agcgtggtgg cagcaggctg
       61 cgagtccgcc ggcagcgatt ctaacagtgg tcgatcgatc aggtctgtcc catttgcgcg
      121 ctacagcgcg caggagttag cgaccgcatc agagagcctt ttcgtcggca cctttggcga
      181 cgtagatcga cgcaccacgg gcaatgggct gcgtcgccga tggcgtcgaa acaagatcag
      241 gagaaacgcc gcaagctacc gcggaaggag cgcgttctgg acaagtgtgg atcgacacca
      301 ggccgcggta tggaaatgcc aactcgcccg gcgggtcttg atccgattgt ggcggagatc
      361 atcgaagcac ttgcacgaac catcatcaga gatgaagatc gtcgaaccac cttgcttttc
      421 atgaacaaga acgaataggc ccgaaccatg cgtaagcgcg ccgccattta cgcccggttt
      481 tcatctgacc gacagaggga ttgatcgatc gacgatcagg tcgcgctatg ccaagcgctt
      541 gcggatcgga actcttgggc cgtcgccatg atctacgcgg attttgccgc ctccggatcg
      601 accgtgcacc gtcggccgga attctctcgg atgctgacgg acgcagagga tcaccagttc
      661 gatatcatca ttgccgaaga catcgacagg ttggcgcgag gagaaggtga tgcacccaaa
      721 ctgcgacagc ggttggaatt tctgggaatc gagatgcaca cgtgtaccga cggccatatc
      781 accaaactgc acgctggcct aaagggtcta atgagttcgc tcttcctgga taacctcata
      841 gcacacacga agcgggggat ggccggtgta gtccgtgacg gccgacaccc gggtggaaaa
      901 atatatgggt acagaacgat agcgggccgc cgaggcgagc ttgagatcgt tgaggaagag
      961 gcagcagtcg tacgacagat tttcgagcgc tatcttgcag gagccacgcc gcgccagatc
     1021 gctggccagc tcaatgatga ccgcgtgttg cccccgcgag gcggcgagac ttggcgcgca
     1081 tcaacgatca acggcaacac gcggcgtgct aacggaattc tccagagcga gatttacaga
     1141 ggggttattg tctggaatcg gctgaagatg atacgcgacc catctactgg gcgacggatc
     1201 agccgtccga acgatcgtag cgaggtccgg cgcgtttcag cccctcacct gcggatcatc
     1261 tccgacgaaa cgttccggga cgcacagaag cgcaagtcgg aaagagctca catccaaccc
     1321 agcatacgaa aacgtgcgaa gcggttgctg tccggcctcc tgcgctgcgg ctgttgcggc
     1381 ggtgggatgt cggccgcaag caccggggga cgcacgcgga ttgtgtgtac gaggatgcac
     1441 gagtccggaa cctgcgagaa ccgaaggtac tactaccttg atggaatcga gcgtcgtgtt
     1501 atcgacggtc tacactccaa atttgggacc cgcgaggcaa tcgcctattt cgtcgaagtc
     1561 tacaatgctg agcgcaagag ggcgtcgaag gccggcagga gacagtcggt cgatctcgaa
     1621 cgcaagctcg cgcttgcgga gcgcgatctg aagcgagcca tcgatgcaat gatccggggc
     1681 acgatctcgg aggctgaagg cgatcggatc atccccgaat tgcgccgcca gcgagatcgt
     1741 gcgaaagccg ctctcgcatc ggccgccaaa ccaccaaagg tcatcgagtt gcacccacca
     1801 tcggtcgatg agtacctgcg ggcgatcgaa agactggccg attacgccaa tggtcaactg
     1861 gccaagagtg atgacgagct tgcgcaatcc atacgcaggt tcatcgacag cgtcacggta
     1921 atgccggcag gatcgggcga tgagccagaa atcaaggttg cgggcggcct cgcctcgctg
     1981 ttgccccccg aatcgagggg ttcagggctg ccgggagagg cggtagcggg ggcgcgctac
     2041 agccgttgta aatcgcctgt tgatggggct tttgtattcg cggcctaaaa taggcttaac
     2101 cgtcggtcct tccagcaaaa gtcgctaagc tcacctttcg gccaggtcga tccattcaat
     2161 ctccccagtc gtcatataga catcccgggc atcagacggg agtagccgcc tctattcccc
     2221 tcttgagagc aaattgggcg gccaatgcaa gcccgaacgg ccaaacgaag caggagagcg
     2281 gcgacaatcg caacccctag ttggagacga gtacatgacc caggcttgtg ccgttctgga
     2341 tcctgcacag gttcagcccc gcgcccgtca gtcataggcg atgccggagg catagacgcc
     2401 cacatagagc gaagccgtca atgcgaggac gtatacgatc caatatcgca agggggtttt
     2461 cgcccgccgc atgtcttctg agctcgatgc cgaaacgtc
//
LOCUS       DATLJT010001457         2909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2659634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001457 DATLJT010000000
VERSION     DATLJT010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VM910_33445"
     CDS             complement(<1..161)
                     /locus_tag="VM910_33445"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666447.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HVI67448.1"
                     /translation="MTVRSVVLGCGSYLPKRILTNAELATRIDTSDEWIVQRTGIRQR
                     HIAADDEFTS"
     gene            complement(158..1219)
                     /gene="plsX"
                     /locus_tag="VM910_33450"
     CDS             complement(158..1219)
                     /gene="plsX"
                     /locus_tag="VM910_33450"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HVI67449.1"
                     /translation="MPQKVRIALDAMGGDVGAPVVVPGAAISLSRHPDAEFLLFGDRT
                     LIERELAKYPDLKAASRIIHTDVAVSMKDKPSQALRRGRKTSSMWLAIDAVKKGEADV
                     AVSAGNTGALIAMARFHLRTLPGIDRPAIAGLWPTMRGDSVVLDLGATIGGDAHHLVA
                     LAVMGSAIARVLFNLERPTVGLLNIGVEEIKGHEEIREAAEMLRAMNLRELDYVGFIE
                     GDRIGKGAADVIVTEGFSGNIALKAAEGTARQISEYLRNAMARTWRSRIGYLFARNAF
                     RALRDKMDPGKSNGGVLIGLRGVVVKSHGGADAEGFAYAVDVGYEMVRYDLLTKINQM
                     LNRDGGALASAPTAREAVS"
     gene            complement(1484..2053)
                     /locus_tag="VM910_33455"
     CDS             complement(1484..2053)
                     /locus_tag="VM910_33455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HVI67450.1"
                     /translation="MRRTNAADPWRAPVIVAQIPEIGLSRNLEADKPTRDAMAKIAGL
                     REILSAHAEFDVKPESDGRVRVAGRLVARIGQTCVVTLDPIENNIDETIDLIFAPPEQ
                     IPDRADDDADEGPGAEDQMEPIENGIIDLGRLATDVLYLAIDPYPRKPGAVFEPRVEA
                     ADPEDHPFAALKTLKLDPSRSSPGRPKGK"
     gene            complement(2050..2601)
                     /locus_tag="VM910_33460"
     CDS             complement(2050..2601)
                     /locus_tag="VM910_33460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HVI67451.1"
                     /translation="MLWPFNHFRKPPLVSPGTIEAIYGTIVTQAREPLFYRELGVPDT
                     VNGRFDLLVLHLWMVLRRSRPMAGGGELSQALFDRFCEDLDANLREMGVGDLAVAKRM
                     QAFGEAFYGRSAAYDLAVSDGREALAQALCRNILNGVDMENARRLAAYVETVIAVLGG
                     YDDTTLTNASWTFPLPYSKDLPS"
     gene            2722..>2909
                     /locus_tag="VM910_33465"
     CDS             2722..>2909
                     /locus_tag="VM910_33465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HVI67452.1"
                     /translation="MTETNATREYLPRGLSARWRGLRTAVAAAVVCAALGACTGEQFQ
                     KGYILPPGALEQIPIGAS"
BASE COUNT          566 a          942 c          853 g          548 t
ORIGIN      
        1 gaggtgaact cgtcgtcggc cgcgatatgg cgttggcgga ttccggtccg ctgtacgatc
       61 cactcgtcgg aagtatcgat ccgtgtcgcc agttcggcgt tggtcaatat ccgcttcggc
      121 agataggaac cgcagccgag caccaccgaa cgtacggtca cgagacagcc tcccgagcgg
      181 tcggcgccga tgccagcgcc ccgccatcgc gattgagcat ttgattaatc ttggtcagga
      241 gatcgtagcg gaccatctca tagccaacat cgacggcata ggcaaagcct tctgcatcgg
      301 ccccgccgtg gctcttgacg acgacgccac gcaagccaat cagcacgcca ccattggact
      361 tgccgggatc catcttgtcg cgcagcgccc ggaaggcgtt tcgcgcaaac aggtagccga
      421 tccgtgaacg ccaggtgcgg gccatggcgt tgcgtaaata ttccgagatt tgccgcgcgg
      481 ttccctcggc ggctttgagg gcaatgttgc cgctgaagcc ctcggtcacg atgacgtcgg
      541 cggcaccctt tccgattctg tcgccctcaa tgaaacccac ataatcgagt tctcggaggt
      601 tcatcgctcg cagcatttca gccgcctcgc ggatctcttc gtgccctttg atttcctcga
      661 ccccgatatt gagcaggccg acggtcggcc gctcgaggtt gaacagaacg cgcgctatcg
      721 cgctgcccat gaccgcaagc gccaccagat gatgtgcgtc gccaccgatc gtagcaccga
      781 ggtccagcac gacggagtcc ccgcgcatgg tcggccaaag ccccgcaatc gccgggcggt
      841 cgatgccggg caaggtccgc aagtgaaagc gtgccatcgc gatgagcgca cctgtattgc
      901 cggcagacac ggcaacatcg gcctcgccct tctttacggc atcgatcgca agccacatcg
      961 atgaggtctt gcggccgcgc cgcagggcct ggctcggctt gtccttcata ctgaccgcca
     1021 catcggtatg gatgatgcgg gaggccgcct taaggtccgg atatttggca agctcccgtt
     1081 cgatcagcgt gcggtcgcca aacagcagaa actctgcatc gggatgtcgt gacaacgaaa
     1141 tcgcggcgcc gggaacgaca acgggcgcgc cgacatcgcc acccatggcg tcaagcgcga
     1201 ttcgaacctt ttgaggcatg aatgtcccgg aaacctgatg tcgagcggct gccgctgccg
     1261 gcggcaatcg gccagacgcg ctgccgcccg gccgggccgc gacaatagcg tgtccccgcc
     1321 ccgacacaac ctcttgaccc ctgtcttttg ttgcgaggga accgaggaca gggaatcgcg
     1381 tctgcgccgc aaactgaaca aatccaatat cttaggggat ggcttcgaag tgtcccgaaa
     1441 ccggcctcgg gcgaccaggc tgcaaaggcg tgcgagatgt acttcacttg cctttcggcc
     1501 ttcccggact tgacctgctc ggatcgagct tgagcgtttt gagggccgca aaaggatgat
     1561 cctccggatc agcagcttcg acacgcggtt cgaagacagc acccggctta cgtggatagg
     1621 gatcgatcgc gagatacagc acgtcggttg cgagccgccc gagatcgatg atgccgtttt
     1681 cgatcggctc catctggtcc tcggcgcccg ggccctcatc cgcatcgtca tctgcacgat
     1741 ccggtatttg ttccggcggc gcgaaaatca aatcgatcgt ttcgtcgata ttgttctcga
     1801 tcggatcaag cgtcaccaca catgtctgtc caatccgcgc cacgagccga cctgcgacac
     1861 gtaccctgcc atcgctctcc ggtttgacat cgaactccgc gtgcgcagag agaatttcgc
     1921 gcaggcccgc aatcttggcc atcgcatcgc gggtgggctt atcagcctcg agattccggg
     1981 aaagtccgat ttccggtatc tgcgccacaa tcacgggagc gcgccacgga tcggctgcgt
     2041 ttgttcgtct catgaaggca agtccttgct gtacggcaga ggaaaggtcc acgacgcatt
     2101 cgtcagcgtc gtatcgtcgt agcccccgag gaccgcgatc accgtctcga cataggcggc
     2161 aagtcgccgc gcgttctcca tgtccacgcc gttcaagata ttccgacaca gcgcttgcgc
     2221 cagggcctcg cggccatcgc tcacggcgag atcatacgca gcagaccgcc catagaaggc
     2281 ttcgccgaac gcctgcatcc gtttcgccac ggcgagatcg ccgacgccca tttcgcggag
     2341 attcgcgtcc agatcttcac aaaagcggtc gaacagcgcc tgagagagct cgcctccgcc
     2401 agccattggc cgtgagcgcc gcaataccat ccacaaatgc agcaccagca ggtcaaaacg
     2461 gccgttaacc gtatccggca cacccaactc ccggtaaaat aacggttctc gcgcctgcgt
     2521 cacgatcgtg ccatagatgg cctcaatggt gccgggtgag actagcgggg gtttcctgaa
     2581 gtgattgaac ggccaaagca ttatgggttc cacaagcgag cccctgcacc tggcgggcgc
     2641 gcgcatcttg caatccgcac cactatcccg ataggtcagc ggcaggctgg ccggttctag
     2701 gaggacggga cacgttccgc aatgactgag acgaatgcga cccgcgagta tctgccccgc
     2761 ggcctttccg cgcgctggcg tggcctgcgc acggccgtgg ccgcagcggt cgtttgcgcg
     2821 gctctgggcg cttgcacggg cgagcagttc cagaagggct atatcctgcc gccgggtgca
     2881 ctcgaacaga tcccgattgg tgcaagcca
//
LOCUS       DATLJT010001458        10165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2887489,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001458 DATLJT010000000
VERSION     DATLJT010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887489"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VM910_33470"
     CDS             complement(<1..642)
                     /locus_tag="VM910_33470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI67453.1"
                     /translation="MPWDDRTKRRLKLRDLDVLLAVVETGSMGKAAKRLNISQPAVSK
                     AVVELEDALGVRLVDRSRRGVVPTLYGLALAKHSVAIFNDLRQGVQDIDFLSDPTKGE
                     IRIGTTEPIATAIVLPAIDRLSRKYPQMFFHVVPGDTSSLYKDVSERNIEFAICRMIG
                     RLPDELAADVLFYDVFSIVTSAKNPLTRRRKLTLADLRNEPWALLPFDSFFGSV"
     gene            804..1781
                     /locus_tag="VM910_33475"
     CDS             804..1781
                     /locus_tag="VM910_33475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67454.1"
                     /translation="MNRREFLAGAAAIGCGGAAFGSAFAQSFPSNVIRILVPASASTP
                     PDILARIIASALSEGEGWKVVVENKPGAVMTIGTMEALNQPADGHTLLSVTAPIAAVP
                     GLVPNAQFHLETDFAPLIQTGTGYNVLVVNPSIPVHSVPELIAFLKKDPGKHTFSSGG
                     FGTPAHLLGELFKLETGVQATHVPYVQFPQAIGDLVNGVNTYQFITVLPVVQLIKTGK
                     LRALAVMGHKRVAALEDVPTIVEAGYPKLASEDWAGILVKSGTPAPVIAQLNAAINKA
                     IKTDKVRDSLAKLGVDPGGGSPEAFGTLVHTEIAHWSKVIKDAGIKINS"
     gene            1778..2698
                     /locus_tag="VM910_33480"
     CDS             1778..2698
                     /locus_tag="VM910_33480"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI67455.1"
                     /translation="MTSSDFRVTLLGTGVPIPSSDRFGPCTLVEAGEQKFLIDAGRGA
                     TIRLHQLKIPIGRIDMQLLTHYHSDHTSGIPDVWLTGWLQSYFGTRKTPYRVIGPTGA
                     RALMENLERAYALDIKIRAADEKLPLSGIATDIVEFDRDGVVYEKDGVTVVAFEVDHG
                     DVIKPCYGYRFEYAGRTAVFSSDTRYNQNVIKYGAGADLLVHEVASARPELMKEAYVQ
                     RIIGHHTTPREAGRVFAQAKPKLAAYTHIVLVGSERIPPPTIDDVLAETRQTYRGPLA
                     VGEDLMAFEIGETVTIRRPQLPSRPATVQA"
     gene            2871..4487
                     /locus_tag="VM910_33485"
     CDS             2871..4487
                     /locus_tag="VM910_33485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVI67456.1"
                     /translation="MHDFNRRDFLRGSTIAAAASTFGLLVPADAASAPTLPSSPQWDA
                     GRVRHLLPTVSDNRVLVKASFRSPLMEAPMLRVGGTTVRGRMTDTRGEFWQFHAADLK
                     PGRPYTLALVGGRRRALCEPWSLTTFPAPDEKPQAFRVLFYTCAGGHEAFGFLPAAVR
                     NRLLRRALSFAPSAMVAIGDQVYWDLLAPENGPKMGASPAGERIAGKFNRSALVLGSD
                     NETVLKRAVEPQLIEVYGTDFRSTPVFFMQDDHDYFDNDEATDEVITFPPPYFSLQLA
                     RATQSLFYPEFLPDRARPLGLPWSSAGDRFGGVSETFGTVRYGRLAEILLYDIRRTQT
                     LAGPSAVYLDLEVEKWLKARTATREVTHVVHVPSNPPGWTAGKWGEWYPDVLGPDGKL
                     TTAKPKPYWQPGWLKQHDRLMESISAVRERVPLVMSGDLHAVAIGRMMRSGKLDFSVN
                     PINAVLTGPISTRPSGWPSARRGTGALPPAHLDMDEEVKPIEQHGFTIADFAPDKIVL
                     RMFKWDVKTQSVEAIDNLDPFHTAVLTRGA"
     gene            complement(4531..5580)
                     /locus_tag="VM910_33490"
     CDS             complement(4531..5580)
                     /locus_tag="VM910_33490"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVI67457.1"
                     /translation="MMKRRTFLGGALTTISAGSLGARSATAQAVVPAPAEPLVWPVVT
                     KVPPGIRALAGHTDTVPDIVGRIGTPPSLVVFTEGNHLMALLSDDILGAFPPWAKSQP
                     RYADLDLDNIVVVTVPQPIVVQMIRTGGLALGNLLLEVNRKSGFYPDIVMAGPEPLRQ
                     LRNLGAIEPQARFFSRNRGFALLVRKGNPLGIHGLVDVARRGARIALPEAASEPQARA
                     RYRAAVDDLIGKPAADELFAAETPHFPGRIGIVHCDLPEMVARGYADVALTQYHLISY
                     WTRIFPNHFALVPVSGAERFFVKIAFGRVVNPLRPRALMAFEEFFFSQARDVYPRYDF
                     ARMNDDEYAAPLALD"
     gene            complement(5727..6209)
                     /locus_tag="VM910_33495"
     CDS             complement(5727..6209)
                     /locus_tag="VM910_33495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67458.1"
                     /translation="MKKLIIPALALTVVQSTALAAPGTASGPSALALAAVIAQHSPAV
                     RAFDKRVIARLFRGDTRFGFTPNTKISVDADTVVCRVSNVDITSRSCELSFGARKRTL
                     TGREANEIGATAAAAGIPSEGAAGSSIESVSKLRCTIDPNEIMQKAGGGAQCSFETGQ
                     "
     gene            6585..7064
                     /locus_tag="VM910_33500"
     CDS             6585..7064
                     /locus_tag="VM910_33500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67459.1"
                     /translation="MRWKTKTFAFVFVLIGSLGYTTASRGEDSRDPSHFTYARLYCAP
                     DGNTHFQDMTVELRKTNFAPPAPPIHIGSDFATSRAFFGGFDAGWGAQDLENRLNHPT
                     PATQFGIVLQGTFSVTTTDGETRRLRPGSVFRLEDTSPCKGHITVVGEEPGFLMFVR"
     gene            7235..8464
                     /locus_tag="VM910_33505"
     CDS             7235..8464
                     /locus_tag="VM910_33505"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67460.1"
                     /translation="MNEASFAELSEWLTQAGLAGTSETDILSGCCDRCIAAGLPLARA
                     HMFVDTLHPVHEGRLFRWGYGDSPLLEYGRTSPDALAASGSAPRDVEAARVWRDSPHY
                     KMMQTGDSFLRRRLNATAKDEFPVLADFVAAGMTDYVAIISRFAPEGVIGEMDGVYSS
                     WATSVPEGFSDGQVAALERIAPYLALAIKSVSLARMTATLMETYLGRDAGRRVLGGRI
                     VRGIAERIDAVVWFSDLRGFTRITDTAPEHAIPLLNDYSDAIVSAIHEHGGDVLKLIG
                     DGTFAIFTAEDRAHACGAALSAAIAARARVEELNKSRAADGKPITHMYLGLHVGEVFY
                     GNVGSRERLDFTVIGPAVNEASRIAAMCRSVDQPVLMSSAFADVGGINRRLVSVGRYA
                     LRGVAHPQELFTLDPDG"
     gene            8522..9493
                     /locus_tag="VM910_33510"
     CDS             8522..9493
                     /locus_tag="VM910_33510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67461.1"
                     /translation="MHRMIAAAAMVMALGAHAAAQDWPTRPITLIVPFAAGGGIDVSA
                     RIQAQRMGELLGQSIVIENVGAAAGMAGGVRVAKAPPDGYTLLIGNTGTHAFNQSLYK
                     KPLYNAATDFEPVGLVSESPRILIARKDLPADNLTQFIAYLKANEKTAQFGSAGVGSG
                     THLPCVLLNLAIGVAPTHVPYRGEGPVTQDLIGGRIDYLCSTIQNGAAQANQGAVKAI
                     AVMAQKRVPIIPDVATTGEQGLPGVEASVWNAFFFPKGTPEPIVRKLNKAMSDTLDDP
                     AIRRRLEELGLEIVPPERRAPDYLAKYLPAEIERWAKPIHAAGISAD"
     gene            9585..>10165
                     /locus_tag="VM910_33515"
     CDS             9585..>10165
                     /locus_tag="VM910_33515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67462.1"
                     /translation="MLRANAVAAVAFLLGLSATAGAQGWPSRAVTMVVPYAAGGPVDT
                     LGRILAARLSEILGQQVVVENVAGAGGMTGSSRVAKAPPDGYTVLLSGSAVLAINQTL
                     YKKPLYNAVTDFEHVALFSDSARVLITRKDLPVNTLSEFVAYAKANQGKMQYGSAGAG
                     SGMHVCAVLLDGAMGTKITHVPYRGSAPAMQDL"
BASE COUNT         1873 a         3256 c         3213 g         1823 t
ORIGIN      
        1 caccgacccg aaaaaactat cgaacggaag gagcgcccat ggttcgttgc ggagatcggc
       61 aagcgtgagc ttgcggcgcc gcgtcaaagg attttttgct gaagtgacta ttgaaaaaac
      121 gtcgtagaag agcacatctg cagcaagttc gtcgggcaat cgaccgatca tccggcaaat
      181 cgcaaattcg atgtttcgtt cactgacgtc tttgtagagc gacgacgtat cgccggggac
      241 gacgtgaaag aacatctgcg ggtatttgcg cgatagcctg tcgatcgcgg gcaagacaat
      301 tgccgtcgcg attggttcgg tcgttccgat gcggatttcg cccttggtcg gatcggaaag
      361 aaaatcgatg tcttgaaccc cttgtcgcag gtcgttgaag atcgcgacgc tgtgtttcgc
      421 gagcgcaaga ccgtagagtg tcggcacgac gccgcgccgg ctgcggtcca cgagtcgaac
      481 gccgagtgcg tcttccagtt cgacgacggc cttggaaaca gcgggctggg agatgttgag
      541 gcgctttgcc gcctttccca tgctgccggt ttcaaccacg gccaacagaa catcgaggtc
      601 acgcagtttc aggcgacgct tggtgcgatc gtcccacggc atgaaatcgc tcattcccga
      661 atgttatgag acgataccat aataatatta ttccataatg aacgctcggg gtagtctttc
      721 cctcgagcct cggacgggtg cagtctcaaa cactcactcg agaggcgtcg cggacaggca
      781 gccagcaaac gaagggaagc gccatgaata gacgagaatt tttggcggga gcggcggcga
      841 ttggttgcgg cggcgctgcc tttggatcgg catttgcaca atccttcccg tcgaacgtca
      901 ttcgtatcct ggtgccggcg tcggcgagca cgccgcccga cattctcgcg cgcatcatcg
      961 ctagcgccct gtcggaggga gaagggtgga aagtggtggt cgagaacaag cccggcgccg
     1021 tgatgaccat cggcacgatg gaggccctca atcagcccgc tgacggtcat accctgttgt
     1081 cggtcaccgc cccgattgcg gctgttccgg ggctcgttcc caacgcacag ttccatctgg
     1141 agacagactt cgcgccgctg atccaaaccg gcactggcta taacgtgctg gtggtcaacc
     1201 cgtcgattcc ggtccattcg gtgcctgaac tgatcgcctt cctcaaaaaa gatcccggca
     1261 agcacacgtt ctcatcggga gggttcggta cgccggcgca tctgctcggc gagttattca
     1321 agctcgaaac gggggtacag gcgacgcacg tgccctacgt gcagtttccg caagcgatcg
     1381 gcgaccttgt caacggcgtc aacacctatc agttcataac cgtgctaccg gtcgtgcagc
     1441 tcatcaagac cggcaagttg cgtgcgctcg cggtcatggg gcacaagcgt gttgctgcgc
     1501 tcgaggatgt gcccaccatt gtcgaggcag gctatccgaa gctcgcaagc gaggactggg
     1561 ccggcatctt ggtaaagtcg ggaacgccgg cgccggtgat cgcgcagctc aatgccgcca
     1621 tcaacaaggc gatcaagacc gacaaggtgc gcgactctct cgccaagctc ggcgtcgatc
     1681 cggggggcgg ctcgcccgag gcgttcggca cgctggtgca caccgaaatt gcgcattggt
     1741 ccaaggtcat caaggacgcc ggtatcaaga tcaattcatg acgagttccg acttccgggt
     1801 gacactgctc gggaccggcg tgccgattcc gagctcggac cgtttcggcc cctgcacact
     1861 ggtcgaggcc ggcgagcaga aattcctgat cgatgccggc cgcggcgcga ccatccggct
     1921 gcatcagctc aagattccga tcggccggat cgatatgcaa ctgctcaccc actatcactc
     1981 cgaccacacc tcgggcatcc cggacgtatg gctcaccggc tggctgcagt cgtatttcgg
     2041 cacgcgcaag acgccgtacc gcgtcatcgg accgaccggc gcgcgcgcgc tgatggagaa
     2101 cctggagcgg gcctacgcgc tcgatatcaa gattcgcgcc gccgacgaaa agctgccgct
     2161 ttcgggaatc gcgacagata tcgtcgagtt cgatcgcgac ggcgtggtct acgagaagga
     2221 cggcgtgacg gtcgtcgcgt tcgaggtcga ccacggggac gtcatcaagc cctgttacgg
     2281 ttatcgcttt gagtacgcag ggcgcaccgc agtattctcc agcgacacgc gctacaacca
     2341 aaatgtcatc aagtatggcg ccggcgccga cttgctggtg catgaggttg cgagcgcacg
     2401 gccggagctc atgaaggaag cctacgtcca gaggatcatc gggcaccata cgacgccgcg
     2461 cgaggccggc cgcgtcttcg ctcaagcgaa acccaagctt gcagcctata cgcacattgt
     2521 cctggttggc agcgaacgaa ttccgccgcc gaccatcgac gatgttctcg ccgaaacgcg
     2581 acaaacctac cgcgggccgc tggccgtggg cgaagatttg atggcattcg agattggcga
     2641 gacggtcacc attcggcggc ctcaactgcc ctctcggcca gcaaccgtgc aagcgtagca
     2701 taactggcgt gtcggccgat gcgattgctg ctccggggca ttcgccgagc gacccgctgc
     2761 cggccgcgct ggcttccact tgcgctgtgg cgcggtgtaa tctcatcgca gagcccgctc
     2821 ctgcaaagcg cggaaatgcc aggccgatcg aacgggctta cgggaactga atgcacgact
     2881 tcaatcgccg cgatttcctg cgcgggtcga cgattgcggc agccgcctca acgttcggcc
     2941 tgttggtgcc cgccgatgcc gcatcggcgc caaccttgcc gtcgtcgcct caatgggacg
     3001 ctgggcgcgt gcggcatctc ctgccgacgg tgagcgacaa ccgcgttctg gtgaaggcgt
     3061 ccttccgttc cccgctgatg gaggcgccga tgctgcgcgt cggcggcacg acggtccgcg
     3121 ggcgaatgac cgacacgcgc ggcgagtttt ggcagttcca tgccgcggac ctcaaacccg
     3181 gccgccccta cacccttgca ctcgtgggcg gtcgccgccg cgctctctgc gagccatgga
     3241 gcctcacaac ctttccggct cccgacgaaa agccgcaggc gtttcgcgtg ctgttctata
     3301 cctgcgcggg cggccatgag gctttcggtt tcttgcctgc agccgtacgc aaccgactgc
     3361 tgcgccgcgc tttgtcgttt gcgccgtcgg ccatggtggc gatcggtgat caggtctatt
     3421 gggatttgct tgcgcctgag aacggtccaa aaatgggcgc gtctcccgcg ggggaacgca
     3481 tcgccggaaa attcaaccgc tcggcgctgg tgctcgggag cgacaacgag accgtgctga
     3541 aacgagccgt cgaaccgcag ttgattgaag tttacggcac cgacttccgc tcaactccgg
     3601 ttttcttcat gcaagatgat cacgattatt tcgacaacga cgaagccacc gacgaggtca
     3661 taacgtttcc tccgccgtat ttttccctgc agctcgcccg cgccacgcaa agtcttttct
     3721 atcccgagtt tctgcccgat cgagcgcgcc cgctcggcct accatggtcc tcggccggcg
     3781 accggttcgg cggcgtgtcg gaaacattcg gcacggtccg ctacgggcgg cttgccgaga
     3841 ttttgctgta cgacattcgg cgcacccaga cgctcgccgg cccgagcgcc gtctatctag
     3901 atctggaagt cgagaagtgg ctcaaggctc gcaccgcaac acgcgaggtc acccatgtgg
     3961 tgcatgtgcc gtccaatccg cccggctgga ctgcgggcaa atggggcgaa tggtacccgg
     4021 acgtgctggg gcccgacggc aagctcacga ccgcaaagcc caagccttat tggcaaccag
     4081 gctggctcaa gcagcacgat cgcctcatgg aatcgatatc ggccgtgcgc gaacgcgtgc
     4141 cactggtgat gagcggcgac ctgcatgccg tcgccatcgg ccgcatgatg cgcagcggca
     4201 agctcgattt ctccgtcaat ccgatcaatg ccgtgttgac cggtccgatc agcacgcgtc
     4261 caagcggctg gccgtcggcg cgccggggca ccggcgcgct gccgcctgcc catctcgaca
     4321 tggacgaaga ggtaaagccg atcgagcagc acggcttcac catcgcggat tttgcgcccg
     4381 acaagatcgt gctgcggatg ttcaagtggg atgtgaagac gcaaagcgtc gaggcgatcg
     4441 acaacctcga tccattccac acggcggtgc tgacgcgtgg agcgtaaagc gagccccaga
     4501 ccggcttgag cctggccgcg ctgcctccaa tcagtcgagc gccaagggcg ccgcgtattc
     4561 gtcgtcattc atgcgcgcga aatcatagcg cggatagacg tctctcgcct ggctgaagaa
     4621 gaactcctcg aaggccatga gcgcgcgcgg acgcagcgga ttgacgacgc ggccgaaggc
     4681 gatcttcaca aaaaaccgct cggcgcccga aacgggtacg agcgcgaagt ggtttgggaa
     4741 tatccgcgtc caataggaaa tgaggtgata ctgcgtgagg gctacgtcgg cgtagccgcg
     4801 ggccaccatc tccggaagat cgcaatgcac aatgccgatg cggcccggga agtgcggtgt
     4861 ctcggcggcg aagagctcgt cggccgccgg ctttccgatc agatcgtcga ccgcggcgcg
     4921 gtacctggcg cgagcctgcg gctcgctcgc cgcctccggg agcgcgatcc gcgcgccgcg
     4981 acgcgcgaca tcgacgaggc catgaattcc gagtggattg cctttgcgaa caagcagcgc
     5041 gaagccgcga ttgcgtgaga agaagcgcgc ctgcggctcg atcgcgccga ggttgcgcag
     5101 ttggcgcaaa ggctcgggcc cggccatcac gatatcgggg tagaagccgg acttgcggtt
     5161 gacctccagc aggaggttgc caagcgctag tcctccggtg cggatcatct gtacgacgat
     5221 cggctggggg acggtcacca ccacgatatt gtcgagatcg agatcggcgt atcgcggctg
     5281 cgacttcgcc cacggcggga acgcgccgag gatgtcgtcg ctgagcagcg ccatcaggtg
     5341 attgccttcg gtaaagacca ccaggctcgg cggcgttccg atccggccga caatgtcggg
     5401 cacggtgtct gtgtgaccgg ccaacgcgcg aatgccgggc ggcaccttcg tcacgaccgg
     5461 ccacacgagc ggctcagccg gtgcgggaac gaccgcttgc gccgttgccg atcgcgcgcc
     5521 gagacttccc gcgctgatgg tcgtcaatgc gccgccgaga aatgtgcgcc gcttcatcat
     5581 gtcgggcctc ccatcgaggc atggtcgtca gaaatttaac aatatggccg agcatccgca
     5641 tcacgtccgt tgcgagtcat tgcgtcgaaa gcgatcgagc gcgtcgatct gtctgaacgc
     5701 gcggcgccga cggagaaacc ggttggttac tgccccgttt cgaaactgca ctgcgccccg
     5761 ccgcccgcct tctgcatgat ctcgttcggg tcgatcgtgc accgcagctt ggagacgctt
     5821 tcgatgctcg agcccgcggc tccctcggag ggaatgcccg cggccgccgc ggttgcgccg
     5881 atttcgttgg cttcgcgccc ggtcagcgtg cgtttgcgcg ctccgaagct gagctcgcag
     5941 ctgcgcgacg tgatgtcgac attgctgacg cggcagacca cggtatccgc atcgacggaa
     6001 attttcgtgt tgggggtgaa gccgaatctg gtgtcgccgc ggaacaggcg cgcgatgacc
     6061 cgcttgtcga acgcgcgcac cgcgggcgaa tgctgcgcga tgaccgcggc caacgccaag
     6121 gcgctcggcc cgctcgccgt gcctggcgca gccaacgcgg tgctttgcac gacggtgagc
     6181 gccagtgccg gaataatcag cttcttcatc tgtgcagcct ccgttcggaa ccctactgcg
     6241 ctgggcaatc gcgcgcctac ggtgctcgat cgatgcgcct tcccttggcc ccatgcggcg
     6301 aaaccggaat ggcgccgcgt ggaaccaacg agcctcgact cctgccagtt tgcaggattc
     6361 ttgctttggc ctgaatccac aattctgtga tggatcgcga ccggcgaacg cgcgggtgaa
     6421 cataacgctc cgcttcgtgt cgattggacc ggctgccccc tcaggccccg cggagaaagc
     6481 atcgtaacgg cgaagaaata ggacgctgga agaggtagcg ggctgctata gaagccttga
     6541 agggttcgat gtaaacgccg gccaaagcca aggagagtca cccgatgcgc tggaagacca
     6601 agacgttcgc gttcgttttc gttctcatag gctcgttggg gtacaccacc gcatcccgcg
     6661 gcgaggacag ccgcgatcct tcgcatttca cctacgcacg gctctattgc gcgcccgacg
     6721 gaaatacgca ctttcaggac atgacggtgg agctgcgcaa gacgaacttc gcgccgccgg
     6781 cgcctccaat ccacatcgga agcgactttg cgacctcgcg cgcattcttt ggaggatttg
     6841 acgcgggctg gggcgctcag gacctcgaaa accgcctcaa ccatcctacg cccgcaactc
     6901 aattcggcat cgttctgcaa gggacctttt ccgtcaccac gaccgacggt gagaccaggc
     6961 gactgcgtcc cggcagcgtg tttcgattgg aggacacctc gccctgcaag ggacacatca
     7021 ccgtcgtagg cgaagagccc ggattcctca tgttcgttcg gtgagtgccc agcggcgagc
     7081 gccgtccgtc gccgctgggg gtcatccacg gtcgcgggcg tcgaagccgc acgacgtgca
     7141 tgtccgcttc gctccgaaag cgagcgagat cgctgcgctg ccgcgattcg acgcgaggtg
     7201 ccaaaaccgg gcgttgtggc tgtataatcg cggcgtgaac gaagcttcct tcgccgaatt
     7261 gtcggagtgg ctgacccagg ctggcctggc cggcacgtcg gaaaccgaca tcctgtccgg
     7321 atgctgcgac cgttgcatcg cggccggact tccgcttgct cgcgcgcaca tgtttgtcga
     7381 cacgctgcat ccggtgcacg agggccgcct gtttcgctgg ggctatggcg actcgcctct
     7441 gctcgaatat ggccgtacca gcccggacgc gctcgccgcg tcgggctccg cgccccggga
     7501 tgtggaggcg gcccgagtct ggcgcgacag cccgcactac aagatgatgc aaactggcga
     7561 ctcgtttctg cgccgccgcc tcaacgccac cgccaaagac gagtttccgg tgttggcgga
     7621 cttcgtggca gcggggatga ccgactacgt cgcgatcatc agccgctttg cccctgaagg
     7681 tgtcatcggt gagatggacg gcgtgtactc gtcatgggca acgagcgtgc ccgaagggtt
     7741 cagcgacggc caggtcgccg cgcttgagcg catcgcgcca tatctcgcgc ttgccatcaa
     7801 atcggtatcg ctcgcgcgca tgaccgcaac gctgatggaa acgtatctgg gtcgcgacgc
     7861 cggccggcgt gtgctcggcg gacgcatcgt gcgcggaatt gccgagcgca tcgacgcggt
     7921 cgtttggttc agcgatttgc gcggcttcac gcgcatcacc gacaccgcgc cggaacacgc
     7981 gattccctta ctcaacgact actccgatgc aatcgtctcg gccattcacg agcatggcgg
     8041 tgatgtcctc aagctgatcg gcgacggcac atttgccatc ttcacggcgg aagaccgcgc
     8101 gcacgcatgc ggcgcggcat tatccgcagc gatcgccgcg cgcgcgcgcg tcgaagaatt
     8161 gaataaaagc cgggcggccg acggcaaacc gataactcac atgtatctcg gcttgcacgt
     8221 cggcgaagtt ttctatggca atgtcggcag ccgggagcgg ctggacttta ccgtcatcgg
     8281 gccggcggtg aacgaggcaa gccgcatcgc agcgatgtgc cgttcggtcg atcagcccgt
     8341 tctgatgtcg tcggcgtttg ccgatgtcgg cggcatcaac cgccgcctgg tttcggtggg
     8401 acgctacgcg ctgcgcggcg tcgcacaccc gcaggagttg tttacgctcg atcccgatgg
     8461 gtaagcagcc ttgatcgcgg cggcgctggg cgcgaatctg cggcgcgaga aggagacggg
     8521 catgcacagg atgatcgcgg ccgcggcgat ggtcatggca ctcggcgctc atgcagccgc
     8581 gcaggattgg cccacccggc cgatcacgct catcgttccc ttcgccgccg ggggtgggat
     8641 cgacgtcagc gcgcgcatcc aggcgcagcg catgggcgag ttgctggggc aatcaatcgt
     8701 gatcgagaac gtgggcgccg ccgccggcat ggcgggcggc gtgcgcgtcg ccaaggcgcc
     8761 gcccgacgga tatacgctgc tcatcggcaa taccggcacg cacgccttca accagagcct
     8821 ctacaagaag ccgctctaca atgccgcaac cgatttcgag ccggtcgggc tggtatcgga
     8881 atcgccgcgc atcttgatcg cgcgcaagga tctgccggcg gacaacctga cgcaattcat
     8941 cgcctacctg aaagcgaacg agaaaacggc ccagttcggc tcggccggcg tcggctccgg
     9001 gacgcatctt ccctgcgtgc tgctcaacct cgcgatcggg gttgccccca cgcacgttcc
     9061 ctatcgcggc gaggggccgg tgacgcagga cctgatcggc gggcgcatcg actatttgtg
     9121 ctccaccatc cagaacggcg cagcgcaggc caaccaaggc gccgtcaaag ccatcgccgt
     9181 aatggcgcag aagcgggtgc cgatcattcc cgatgtggca accaccggcg agcaaggctt
     9241 gccgggcgtg gaagccagcg tctggaacgc cttcttcttc cccaaaggca cccccgagcc
     9301 gatcgtgcgc aagctcaaca aggcgatgag cgacaccctc gacgacccgg ccatccgccg
     9361 gcggctggag gagctcggcc tcgagatcgt cccgcccgag cgccgcgcgc ccgattatct
     9421 cgccaaatat ctgccggccg agatcgaacg ctgggcgaaa ccgatccacg ccgccggcat
     9481 cagcgccgat tgaaatcgtc tcggttcggc aacacaatcg ctcgcgcaaa cacgatcatt
     9541 cgcaagcgac gcgcaaagcg ttcccgagtc cgcaggagga aagcatgctg agggcgaatg
     9601 ccgtcgccgc ggtcgcgttc ttgctggggc tgtccgcgac ggcgggcgcg cagggctggc
     9661 cgagccgggc ggtgaccatg gtcgtgccct acgccgccgg cggcccggtc gatacgctcg
     9721 ggcgcattct cgcggcgcgg ttgagcgaaa tcctcggcca gcaggtggtg gtcgagaacg
     9781 tcgccggcgc cggcggcatg accggctcga gccgcgtcgc caaggcgccg cccgacggct
     9841 acaccgtcct gctctccggc agcgccgttc tcgccatcaa ccagaccctc tacaagaagc
     9901 cgctctacaa cgcggtcacc gacttcgagc acgtcgcgtt gttctcggac tcggcgcggg
     9961 tgctgatcac gcgcaaggat cttccggtca atacgctttc cgaattcgtc gcctacgcca
    10021 aagccaacca gggcaagatg caatacggct ccgccggggc cggctcgggc atgcacgtgt
    10081 gcgcggttct gctcgacggc gccatgggca ccaagatcac gcacgtgccc tatcgcggct
    10141 cggcgccggc gatgcaggat ttgat
//
LOCUS       DATLJT010001459         1806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1740799,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001459 DATLJT010000000
VERSION     DATLJT010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740799"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VM910_33520"
     CDS             complement(<1..1010)
                     /locus_tag="VM910_33520"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67463.1"
                     /translation="MLARADTVATIAESWLAQFEAALAAPGRPGLERLFHADSHWRDV
                     LALSWHIKTVSGSDAIVRELTTHAGRACPTGCKIDLNRTAPRAVRRAGTDAIEAIFGF
                     ETAQGRGSGVLRLTPDANDGNTFKAWTLLTALDEIKGHEERLGRSRPQGKAYSRDFRG
                     PNWLDLRKAVAEYGDRDPAVLVVGGGQAGLSIAARLAQLGLDTLIVDREARVGDNWRK
                     RYHALVLHNQVHVNHLPYMPFPPNWPTYIPKDKLAAWFEAYVESLELNYWTGTEFEGG
                     GYDEKAKRWSVVLRRADGTQRQMHPRHVVMATGVSGIPSLPDIPSLRDFGGKVLHSSQ
                     YD"
     gene            1130..1543
                     /locus_tag="VM910_33525"
     CDS             1130..1543
                     /locus_tag="VM910_33525"
                     /inference="COORDINATES: protein motif:HMM:NF022833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HVI67464.1"
                     /translation="MGGTVILAWESSQGKVWGTSSRRSSPHVGNFDRQGLGGQADRGY
                     IRSGIHFKTEAFMARLPQRWAPLIYGVIQAAITTGVATAIATLQLTDFKLRFVEEWLF
                     AWGLAFLTMLPVVIFVSPLIQRCVLALTFSPSGEV"
     gene            1555..>1806
                     /locus_tag="VM910_33530"
     CDS             1555..>1806
                     /locus_tag="VM910_33530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67465.1"
                     /translation="MRTKRIFLTAIVAVGIGVAGASLASAQSYPDRLIKIVVPYPPGG
                     PADVAARLVTQPLSARLGQSVIVENQSGGGGRTGAKFVAH"
BASE COUNT          310 a          562 c          558 g          376 t
ORIGIN      
        1 tcgtcgtatt gactggagtg cagaacttta ccgccgaagt cgcgcaagct cggaatatcc
       61 ggaaggctcg gaatgccgct cacgccggtg gccatcacga cgtggcgcgg gtgcatctgc
      121 cgctgcgtgc cgtcggcccg acgcagcaca acggaccatc gttttgcttt ttcgtcgtag
      181 ccgccgcctt cgaattcggt gccggtccaa taattcaact ccaagctttc cacataggcc
      241 tcgaaccagg cggccagctt gtctttggga atgtaggtcg gccagtttgg cggaaagggc
      301 atgtacggca ggtggttgac gtgcacctgg ttgtgcagga ccaacgcgtg atagcgcttg
      361 cgccagttgt cgccgacgcg cgcttcgcgg tcgacgatca gcgtatcgag ccctaattgc
      421 gcgaggcgag cggcgatcga aagcccggcc tgtcccccgc cgacaacgag cacggcgggg
      481 tcgcggtcgc catattcagc gacggccttg cgcaggtcga gccaattggg tccgcgaaaa
      541 tcgcgtgaat aggctttgcc ttgcggccgt gatcgtccaa gccgttcctc gtgtcccttt
      601 atctcgtcaa gtgccgtcag cagcgtccat gccttgaacg tgttgccgtc attggcatcg
      661 ggagtgaggc gcagcacgcc gctgccgcga ccctgcgccg tttcaaagcc aaagatggct
      721 tcgatcgcat cggtgccggc gcgcctcacg gcgcggggcg cggtgcgatt gaggtctatc
      781 ttgcaacccg tcggacacgc gcggccggca tgcgttgtaa gctcgcgcac aatcgcatcc
      841 gagccgctga ccgtcttgat gtgccagctc agggcgagca catcgcgcca atggctgtcg
      901 gcatgaaaca gccgctcgag gccagggcgg cctggcgctg cgagtgctgc ttcgaactgc
      961 gccagccaac tctccgcaat cgtcgcgacg gtgtccgctc tggccagcat tggtgctatc
     1021 tcggttggcg tcggggaaca gtcgggtttg gattgaaact agcatatccg ccgaaggctg
     1081 ccccagatcc agcatcccgg attctgcttc cctcatcggg atgacttgct tgggcggcac
     1141 ggtcatcctg gcgtgggaat cctcgcaagg aaaagtctgg ggcacttcct ctcggcggtc
     1201 atccccacat gtcggtaact ttgatcggca aggccttgga ggccaagcgg accgcggcta
     1261 cattagatcc gggattcatt tcaaaaccga agccttcatg gcacgacttc cgcagcgatg
     1321 ggctccactg atctatggtg ttatccaggc ggcgatcacg acgggcgtgg caaccgcgat
     1381 cgcaaccctc cagttaactg atttcaagct gcgattcgtg gaggaatggc tattcgcttg
     1441 gggactggcc ttcttgacca tgttgccggt tgtgatcttc gtttcaccgc tcattcagcg
     1501 ctgtgtgttg gctttgacct tctctccttc cggcgaagtt tgaactgggc aacgatgcgc
     1561 acgaagcgaa ttttcctcac cgcaatcgtc gccgtcggca tcggcgttgc gggcgcgagt
     1621 ctcgccagcg cccaatccta ccccgaccgg ctgatcaaga tcgtcgtgcc ctatcctccg
     1681 ggcggccctg ccgacgtggc ggcgcgcctc gtgacgcagc cgctctccgc gcgacttggg
     1741 caaagcgtca tcgtcgaaaa tcaatcgggc ggtggcggac ggaccggcgc caagttcgtg
     1801 gcgcac
//
LOCUS       DATLJT010001460         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_591919,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001460 DATLJT010000000
VERSION     DATLJT010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591919"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..938
                     /locus_tag="VM910_33535"
     CDS             6..938
                     /locus_tag="VM910_33535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HVI67466.1"
                     /translation="MIPSRAFVHRVRRHFKAGVDGSLGWLAVGMLRTIRATNRKHMAD
                     FAASFMRTLGPRLKEHKIGRANLAAAFPEKSPAEIETILRGVWDNLGRVAAEFAHIDR
                     LQMFDPDPQEMGDIVYSSEVDKRFRQLRRDGRPALVFAAHLANWELPALIAAKYRLET
                     TVLYRQPNITAVSDAIIQIRKGSMGTLVPTSLDAPFKLLRVLEAGGHVAMIVDQHYVN
                     GVDVTFFGRRCKANPFIARLARHVDCPIHGTRIIRLPDRHRFRVDLTEAIEPPRDADG
                     KIDIEGTMQAITSVVEGWVREHPEQWLWVHRRWR"
     gene            complement(963..2021)
                     /locus_tag="VM910_33540"
     CDS             complement(963..2021)
                     /locus_tag="VM910_33540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI67467.1"
                     /translation="MAITPAAAQPKQRVVNVYNWSDYMAPGVVEDFSKETGIKVRYDT
                     FDSNDTLETKLLAGKSGYDVVVPTAYFLERQIKAGVFQKLDKAKVPNLANMWPEISQR
                     LAAYDPGNQYAVNYMWGTTGIGYNVKKARELLGANGKIDSWDIVFKPENLAKFKDCGV
                     HLLDSSDDILAAALHYLGLDPNTSNEADLQKAADLVSKIRPYVRKFHSSEYLNALASG
                     EICLVVGFSGDVKQSQKRAAEAKNRVEIAYAIPKEGAQLWFDNLGIPRDARNAAEADD
                     FINYLQKPEVAAKNTNFISYANGNLASQKFIDKSILDDKTIYPDDATMRKLYTISAHD
                     PKTQRLMNRLWTRIKTGK"
     gene            complement(2150..2485)
                     /locus_tag="VM910_33545"
     CDS             complement(2150..2485)
                     /locus_tag="VM910_33545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI67468.1"
                     /translation="MSAQLGRENVHDLTPEEVARGLAEGKVLLVDVREPNETEVERYP
                     QAFYLPMSEFDSAAIPDPQGRRVVFACRSGNRSVTASLMAQAAGLPYDAHLAGGIKAW
                     KEVGLPTES"
BASE COUNT          489 a          865 c          841 g          547 t
ORIGIN      
        1 agcccatgat cccgtcgaga gctttcgtcc atcgggtccg ccggcatttc aaggcgggcg
       61 ttgacggctc gctcggttgg ctggcggtgg ggatgttgcg gaccatccgc gcaaccaacc
      121 gcaagcacat ggccgacttc gccgccagct tcatgcgcac gcttggtccg cgactcaagg
      181 agcacaagat cggccgcgcc aacctcgccg ccgcctttcc ggaaaaatcc cccgccgaaa
      241 tcgaaacgat cttgcgcggc gtttgggaca atctcggccg cgttgccgcc gagttcgccc
      301 atatcgatcg cctgcaaatg ttcgatcccg acccgcagga gatgggcgac atcgtctaca
      361 gcagcgaagt cgacaaacga tttcggcaat tacggcgcga cggcaggcct gctctggtgt
      421 tcgccgccca tctcgcgaat tgggaactgc cggcgctgat cgccgccaag tatcgtctcg
      481 agacgacggt gctgtaccgc cagcccaata tcaccgccgt gagcgatgcg atcatccaga
      541 tccgcaaagg cagcatgggc accctcgttc cgaccagcct cgacgcgccg ttcaagctgt
      601 tgcgcgtgct cgaagccggc gggcatgtcg ctatgatcgt cgaccagcac tacgtgaatg
      661 gcgtggacgt gaccttcttc gggcgccgct gcaaagcgaa ccccttcatc gcccgcctgg
      721 cgcggcacgt cgattgcccg attcacggta cgcgcatcat ccgcctgccc gaccgccatc
      781 gctttcgcgt cgatctgacc gaagccatcg agccgccacg cgatgccgac ggcaagatcg
      841 acatcgaggg caccatgcag gcgatcacct cggtggtcga aggctgggtg cgcgagcacc
      901 ccgagcaatg gctgtgggtg catcggcgct ggcggtgacg agaacgtcgc cgcccctcgc
      961 tattacttgc cggtcttgat cctggtccac agccggttca tcagccgctg tgtcttcgga
     1021 tcgtgggcgc tgatggtgta aagctttctc atcgtcgcgt cgtccggata gatggtcttg
     1081 tcgtcgagga tcgatttgtc gatgaatttc tggctcgcca gattgccgtt ggcataggaa
     1141 atgaagttcg tgtttttcgc ggcgacctcg ggcttctgca gataattgat gaagtcgtcc
     1201 gcttcggctg cgttcctggc atcgcgcggg atgccaaggt tatcgaacca caattgcgcg
     1261 ccttccttcg gtatggcgta agcgatctcg accctgttct tggcttccgc ggcgcgcttt
     1321 tgcgactgct tgacatcgcc ggaaaagccc accacgagac agatctcacc ggaggcgagc
     1381 gcgttgaggt actccgatga gtggaacttg cgcacgtagg gcctgatctt gctcacgaga
     1441 tcggcggcct tttgcagatc ggcctcgttg ctggtgttgg ggtcgagccc aagatagtgc
     1501 agcgccgccg ccagaatgtc gtcggaagaa tcgagcaaat gcacgccaca gtccttgaac
     1561 ttggccaggt tctcgggctt gaaaacgata tcccagctgt cgatcttgcc gttcgccccc
     1621 agcagctcgc gcgccttttt gacgttgtag ccgatgccgg tcgtgcccca catgtaattg
     1681 acggcgtact ggttgccggg atcgtacgcc gccaatcgct gtgagatctc cggccacatg
     1741 ttggcgaggt tcggcacctt cgccttgtcc agcttctgga aaacaccggc cttgatctgg
     1801 cgctcgagaa aataggccgt cggcaccacc acgtcgtagc cggatttgcc ggccagaagc
     1861 ttcgtctcga gcgtgtcgtt ggaatcgaat gtgtcgtagc ggaccttgat cccggtctcc
     1921 ttgctgaaat cctcgacgac gcccggagcc atgtagtcgg accaattgta gacgttgacg
     1981 acgcgctgtt tcggctgcgc ggcagcaggc gtgatcgcca tggtggcggc tgcaacgagg
     2041 gcaaaccgga ttgctcggcg catggacggc tccttcagga ccatcgaatg aaaagatcgg
     2101 ggcaggttcg ggagatggcg gaccggctcg cgcgtcgccg caacgcgctt cagctctcgg
     2161 tcggcaaacc gacctccttc cacgctttga tcccgccggc gagatgggcg tcgtagggca
     2221 gccccgcggc ctgcgccatc agcgaagcgg tgaccgaccg gttgcccgag cggcaggcga
     2281 atacgacccg gcggccctgg gggtcgggaa ttgcagccga atcgaactcc gacatcggca
     2341 gatagaaggc ttgcggatag cgctcgactt cggtctcgtt cggctcgcgg acgtcgacga
     2401 gcaggacctt gccttcggcg agcccccgcg cgacctcttc cggggtcaga tcatgcacgt
     2461 tctctcggcc aagctgagcg gacatgggac ttactcctca attcgggcgc ggcgtcgccg
     2521 ctcttgcaaa caagttgcgc gtcgcaatcc aagaatcaag agacccgcga gattttgctg
     2581 ccggccggtg tcccgtctga accgccacac tagcgtcaga tcgtcacctg cgtgcccact
     2641 tccaccaccc gcccggtggg gatctgaaaa tagtcggtcg cgtcgttggc gatgcgcgtg
     2701 agcatgatga acaggcgatc ctgccagcgc ggcatgccgg aa
//
LOCUS       DATLJT010001461         5092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4496061,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001461 DATLJT010000000
VERSION     DATLJT010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4496061"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VM910_33550"
     CDS             complement(<1..948)
                     /locus_tag="VM910_33550"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVI67469.1"
                     /translation="MTQGIGARIQRKEDARHLHGRGNFVSDMILPGQSEVAFLRNPIA
                     HGRIRRVVKPPGTENQIFIRADLGDVAAMVAPTTVPGYKLSEWHPLAYEKVRFVGEAI
                     AMCVAPTRAEAEDLSEQIEVEFDELPVLVEAQAAGMESTVRVHEQWNDNIFLTLNYES
                     GFEVKAKGAAVVIQREIALARQAMVPMEGKAVLAHWDDRSDQLVVYASTQVPHIIRVG
                     LAQFLGIDQGRVRVISPDVGGGFGYKCILQPEELCVAWLALKFRRPFRYIEDRREHLV
                     VGANSRQHHYRLTAHADERGRLLALDADITIDGGAYSAWP"
     gene            complement(1015..2106)
                     /locus_tag="VM910_33555"
     CDS             complement(1015..2106)
                     /locus_tag="VM910_33555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI67470.1"
                     /translation="MRRRAFLMRASAGVAATAIARPAIAQTGAPVRWRLATSWPKSLD
                     TLYGGVDAMCQRVGQMTDRKFQIQCFAGGEIVPPLQVWDATQNGTVECGHTLTSFNIG
                     KNPAVAFDSGLAFGLNTRQQQAWMNYGGGLELIRALFKKDRILPIPCGNVGVQMGGFY
                     RKEINSVDDLKGLKFRIGGLGGMILAKLGVVPLQIPTADIYPSLERGAIDAAEWIGPY
                     DDEKLGLHKVAKYYYYPGWWEGSAQVTLLVNLQHWEALPESYRAPLEAACAETTVMMM
                     AKYDTRNPEALRRLVAAGVQLRQFPRPVLDACYKAAFETYDELAGRNADFKLIYESWQ
                     KFLADSNLWFRIAENTLDNYRFAMSAQPR"
     gene            2392..2868
                     /locus_tag="VM910_33560"
     CDS             2392..2868
                     /locus_tag="VM910_33560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI67471.1"
                     /translation="MRADTVHSDMPLWGRPGYLLRRLHQIHYALFFEECAGFDITPVQ
                     YGLLTTLSVNPDLDQNSLARELGIDRTNVADVLNRLARRGLLERRRSPDDGRMVLARL
                     TRAGERLTRKMYRPMRRAQKRLLEPLLAQERNALIITLIRLINGNNHLGRTIFQPS"
     gene            complement(2921..3565)
                     /gene="maiA"
                     /locus_tag="VM910_33565"
     CDS             complement(2921..3565)
                     /gene="maiA"
                     /locus_tag="VM910_33565"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVI67472.1"
                     /translation="MSRDLYTFFRSSTSYRLRIALAYKRLEYTPHYVSLPKMEHRTPS
                     YRALHAQGLVPFLIDGDRSFIQSMAIIEYLDEVYPEPPLMPADVHGRHYVRAVSQIIG
                     CDIHPLNNVRVLKHIQTQFGADDAATKTWYRHWIAEGLGGLETYVVSTGLARRFCYGD
                     TVTMADICLVPQIFNAQRFDCPLDDYGTLTAIFERCMAVDAFRTTQPNTQADAF"
     gene            complement(3570..3944)
                     /locus_tag="VM910_33570"
     CDS             complement(3570..3944)
                     /locus_tag="VM910_33570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67473.1"
                     /translation="MAQEPLATKGNFLHIYDFRVAKGKEEEFVRSFEKFDYSDGNPMH
                     KSRAQVKDGVLCRDTQDPQRFFLIAEWSDIEEHARIRKILANEIKPEFIKYIEGGTFI
                     PKYVEVVSSTPDEILNKAAAAE"
     gene            complement(4067..4687)
                     /gene="leuD"
                     /locus_tag="VM910_33575"
     CDS             complement(4067..4687)
                     /gene="leuD"
                     /locus_tag="VM910_33575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HVI67474.1"
                     /translation="MDKFIRLTATACPLDVANLNTDQLLPARFLKVPRSAGLATALLR
                     DLRFDSDGRERPDFPLNRPAWRDARIIVGARNFGCGSSREAAVYALFDYGIRCVIAPS
                     FGEIFAGNAVQNGLLTAVASDDEAAALVAMLGENPNRLVTVDLEQQMIQCGDRSFHFA
                     IEPVRRTRLLNGWDNIALTGSYIDKIAAFRRADRARRPWAAPAKPE"
     gene            complement(4695..>5092)
                     /locus_tag="VM910_33580"
     CDS             complement(4695..>5092)
                     /locus_tag="VM910_33580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HVI67475.1"
                     /translation="IDRIFIGSCTNARIEDLRAAAAVLAGRVSKVPGLVSPGSSAVKR
                     QAEEEGLDRTFRDAGLEWGESGCSMCVGINGDVVAPGERCASTTNRNFRGRQGPGART
                     HLMSPAMVAAAAVTGKLTDVRPLLSGRKV"
BASE COUNT          951 a         1564 c         1596 g          981 t
ORIGIN      
        1 cggccacgcc gagtacgcgc cgccgtcgat cgtgatgtcc gcatcgagcg ccaacaacct
       61 gccgcgttcg tcggcatggg cggtcagccg gtagtgatgt tgtcgtgagt tggcgccgac
      121 caccagatgc tcgcggcgat cctcgatgta gcggaacggc cgtcggaatt tcagcgccag
      181 ccacgccacg cacagctctt ccggctgcag gatgcatttg tagccaaagc caccaccgac
      241 atccggcgag atcactcgca cgcgcccctg gtcgatgccg agaaactgcg ccaagccgac
      301 acggataatg tgcgggacct gggtcgaggc atagacgacg agctggtcgc tgcgatcgtc
      361 ccagtgggcg agcacggctt tgccttccat cggcaccatg gcctgacgcg caagcgcgat
      421 ctcgcgctgg atcacgaccg ctgcgccttt ggccttcacc tcgaaaccgc tctcgtagtt
      481 gagcgtgaga aagatgttgt cgttccattg ctcgtggacg cggacagtcg actccatccc
      541 tgccgcctgc gcctcgacga ggaccggcag ctcgtcgaat tcgacctcga tctgttcgct
      601 aagatcctcg gcctcggccc gcgttggggc gacgcacatt gcgatcgcct cgcccacgaa
      661 gcgcaccttc tcgtacgcca acgggtgcca ctcggataat ttgtagcccg gcacggttgt
      721 cggtgcgacc atcgccgcga catcgcccag atccgcgcgg atgaaaatct ggttttcggt
      781 gccgggcggt ttgacgacac gccggatgcg gccatgcgcg atcgggttgc gcaggaaggc
      841 aacttcgctc tgcccaggaa ggatcatatc tgagacgaag ttgccgcgac cgtgcagatg
      901 gcgggcgtcc tccttgcgct ggatccgggc tccgatccct tgcgtcatgc ttgatctcga
      961 tgcaaaggcg accgccgcca acttgcggcc cgtcgcgcgg gcgctgtggt ggcgctatcg
     1021 cggctgcgcc gacatggcga agcgatagtt gtcgagcgtg ttctcggcga tccggaacca
     1081 aagattggag tccgccagga acttctgcca cgactcgtaa atcagcttga aatccgcgtt
     1141 cctgccagcg agctcgtcgt aggtctcgaa cgcggctttg taacatgcgt ccaaaaccgg
     1201 acgtggaaat tgccgcagct gcacgccggc cgccacgagc cggcgcaacg cctcggggtt
     1261 tctggtgtcg tacttggcca tcatcataac ggtcgtctcg gcgcacgccg cctcgagggg
     1321 ggctcgatag gactccggca gggcctccca atgctggaga ttgacgagca gcgtgacctg
     1381 agcgctacct tcccaccagc cgggatagta gtagtacttg gccaccttgt gcaggccgag
     1441 cttctcgtcg tcgtaaggac cgatccactc ggcggcatcg atcgcgccgc gttcgagcga
     1501 tggataaata tcggcggtgg gaatttgcag cggcacaacg ccgagcttcg ccaggatcat
     1561 gccacctagc ccgccgatgc ggaacttgag ccctttgagg tcgtcgacgg agttgatctc
     1621 cttgcggtag aagccaccca tctgcacgcc cacattgccg cagggaatgg gcaggatacg
     1681 gtccttcttg aacaacgcgc ggatgagctc cagccctccg ccgtaattca tccaggcctg
     1741 ctgctggcgc gtgttgagac cgaacgcgag cccggagtcg aacgcgaccg cagggttctt
     1801 gccgatattg aacgaggtga gggtgtggcc gcactcgacg gtgccgtttt gcgttgcatc
     1861 ccaaacttgc agcggcggca cgatttcccc gccagcgaag cactggatct ggaatttcct
     1921 gtcggtcatc tgccccacac gttggcacat ggcgtcgaca ccgccgtaaa gcgtatcgag
     1981 gctctttggc cagctcgtcg cgagccgcca acgcaccggg gcgccagttt gcgcgatcgc
     2041 cggccttgcg atcgcggtcg ccgcgacgcc cgcgctcgct cgcatgagaa acgcacgcct
     2101 tcgcattttt tgtctcctgc cgagatataa ctgcgagaac cggcacggcc ttcggagtcg
     2161 cagtttcttt tttgccggtt gcatctgcgc gcagcgtgga taaaagccta acatagtgcc
     2221 cggtgcgtgc aagtatgacc gtatactgaa tatttcctgc tgcgcaggag gcgagactgg
     2281 tgcgacgagg aggaaagcct aatggcggca agaatgaaag cggcacggaa atacgcgccg
     2341 agacttgcca gcaaaaggaa agcgcgcaga cgcaacgcca agaaaaggcc attgcgtgcc
     2401 gacacggttc attccgacat gccgctgtgg gggcggccgg gttatcttct gcggcgcttg
     2461 catcaaatcc attacgcgct gtttttcgag gagtgcgcgg gattcgacat cacaccggtt
     2521 cagtacggat tgttgacgac gctctcggtc aatcccgatc tcgatcaaaa ctcattggcg
     2581 cgcgaactcg gaatcgaccg caccaatgtc gccgatgtcc ttaaccgcct cgcgcgacgc
     2641 gggctcctcg agcgccgccg cagcccggac gacggacgca tggtgcttgc gcgattgact
     2701 cgcgccggcg agcgattgac gcgaaagatg tatcggccga tgcggcgtgc ccagaagcgt
     2761 ctactcgagc cgcttctcgc gcaggagcgt aacgcgctta tcataacgct gatccgcctc
     2821 atcaatggca acaatcatct tgggcggacg atctttcagc ccagttgagg tcaatgaccg
     2881 agaacgggcg ggtcttgccc gtcccgcggc gcagtccggt tcagaatgcg tcagcctgag
     2941 tgttgggctg ggtcgtgcga aaggcatcga cggccatgca tcgctcgaag atcgcagtca
     3001 gggtgccgta gtcgtcgagc ggacaatcga agcgctgggc gttgaaaatc tgcggcacga
     3061 gacaaatgtc ggccatggtg acggtgtcgc catagcagaa gcgacgggcg aggcccgtgc
     3121 taaccacgta agtctccaga cctcccaatc cctcagcgat ccaatggcga taccacgtct
     3181 tggtcgctgc atcgtcggca ccgaactgcg tctggatgtg cttgagcacg cgtacgttgt
     3241 tgagagggtg aatatcacag ccgatgattt gcgacaccgc tcgcacgtag tggcgtccat
     3301 gcacgtccgc cggcatcagc ggcggctccg ggtagacctc gtccagatac tcgatgatgg
     3361 ccatggactg aatgaacgat cgatcgccat cgatcaggaa gggcactaat ccctgggcat
     3421 gcagcgcccg atatgagggt gttcggtgct ccatttttgg caggctgacg taatgcggcg
     3481 tgtactcgag ccgcttgtag gcgagtgcaa tgcgaagccg gtatgaggtg gaggatcgga
     3541 agaacgtata taaatcgcgc gacaaacgtc tactccgccg cggcggcttt gttgagaatc
     3601 tcgtctggcg tcgacgagac cacctcgaca tatttgggaa tgaacgtccc cccttcgata
     3661 tatttgatga attccggctt gatctcgttg gccagaatct tgcggatgcg cgcgtgctct
     3721 tcgatgtcgg accactccgc gatcagaaag aagcgttgcg gatcctgggt gtcgcggcac
     3781 agcacgccgt ccttcacttg cgcccgtgac ttgtgcatgg ggttgccgtc cgagtagtcg
     3841 aacttctcga acgagcggac gaattcctcc tccttgcctt tggccacgcg aaaatcatag
     3901 atgtgcagaa agttgccctt cgttgcgagg ggttcttgcg ccatgacgtc ctcctgttcc
     3961 tgatcgatac gagcgtacgt tggtcgattt ttgaaagcaa ccgcgccgga gtatgcggag
     4021 agcatgcgtc cgctaactgc cggcggcggc ggcgagatgc aatgcgttat tcaggtttcg
     4081 ctggcgcggc ccatgggcgc cgcgcgcgat cggccctcct gaaggctgcg atcttgtcga
     4141 tgtagctccc ggtcaacgcg atgttatccc aaccgttgag caggcgcgtg cggcgaaccg
     4201 gctcgatggc gaagtggaag cttcgatcac cgcattggat catctgctgc tcgagatcga
     4261 cggtcaccag gcgattcggg ttttcgccca gcatcgcgac gagcgccgcg gcctcatcgt
     4321 ctgaggcgac cgcagtcaat agtccgttct gcacggcgtt gcccgcaaag atctcgccga
     4381 aggatggcgc gatcacgcat cggatgccgt aatcgaagag cgcatagacc gccgcctcgc
     4441 gcgaggaacc acaaccgaaa ttgcgagcgc cgacgatgat cctggcatcg cgccaagccg
     4501 ggcggttgag cggaaaatcc ggccgctcgc gcccgtcgct atcgaagcgc agatcgcgca
     4561 gcagcgcggt tgcgagcccg gccgagcgcg gcaccttcag aaagcgtgcc gggaggagtt
     4621 gatcggtgtt gagattggcg acgtcgagcg ggcaggcggt cgcagtcagc cggatgaatt
     4681 tgtccatgcg cgcctcacac cttccgtccg gagagcagtg gccgcacgtc ggttagcttt
     4741 ccggtcaccg ctgctgccgc gaccatggcc ggagacatca aatgggtgcg cgcgcccggt
     4801 ccctgccgtc cgcggaaatt gcggttggtg gtggaggcgc agcgttcgcc tggcgccacc
     4861 acgtcgccgt tgatgccgac gcacatggag cagccggatt cgccccattc gagacctgcg
     4921 tcgcggaagg tccggtcgag cccttcctcc tcggcctggc gcttgacggc ggacgagcca
     4981 ggcgacacca acccggggac cttgctcacc cggcctgcca ggaccgcggc tgctgcgcgc
     5041 agatcctcga tgcgcgcatt ggtgcaggag ccgatgaaga tgcggtcgat cg
//
LOCUS       DATLJT010001462         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4496103,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001462 DATLJT010000000
VERSION     DATLJT010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4496103"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VM910_33585"
     CDS             complement(<1..621)
                     /locus_tag="VM910_33585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67476.1"
                     /translation="MILRNTSGTYEIYDIGNNAILGGCRLGQVGTDWGYVTVGGFFGS
                     DTTDLLLRNGRTGAFEVYDVSNNNITNAASLGAVGLNRQVMGFGNFSSRGENDMILRN
                     SSTGGVEVYDLANNQITGAAFMGTVGLNWQFSGIGNFSGAGESDMLLRNANTGGLEVY
                     DIANNAITGAAFIGTIGLDWQFSGVGNFSGVPGETDLLLRNSRTGGL"
     gene            768..1106
                     /locus_tag="VM910_33590"
     CDS             768..1106
                     /locus_tag="VM910_33590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67477.1"
                     /translation="MITLLAVGPENDKTVAFTPSYVSVPATPYVSPSGPRIDAIAAAV
                     LVGSTQNWNPNPLALPGTNVSCPAWYSILPVLRAEYATVTDPGETVSASRIQVPVTAL
                     FWIEVLKAVV"
     gene            1502..3064
                     /locus_tag="VM910_33595"
     CDS             1502..3064
                     /locus_tag="VM910_33595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVI67478.1"
                     /translation="MIYRFDDCALDTDRRELRRGGVPCPLEPQVFDLLEFLIRNRDRV
                     VSKEDVFNSVWRGRIVSEAVLHTQLSAVRRAIGDSGSGQRLVRTLRTKGFRFVGAVRE
                     ETRTKRTTRLAEESRNRALLADHPTIAVLPFANISGDPALESLADGMTEDLITALSKV
                     GWLYVATRASSFACKGQVFGTAQAARKLGVRYLLAGALRQEVGRRRITVQLIDAIADH
                     QIWAERYDRDFIDRLAVQDEICEHVVAAIEPQLYIAEQVQVQRKSPANLNGWECIVRA
                     LSLMNSRDRKNVALAQALLRKAVSIDPNSAQGHSLLSIATTLRVHMSWADRQTVVPLA
                     LASAHKALALNPDEPWAHAALGYALIWKRPEDAIVPCQSAVKLDPDFAAGHYFLALGS
                     AYAGCHDRVFPHAAMAERLARRDLLARAYAGAHQNVRATGSFAIERYSEGAEFARSAA
                     VYIPNSPTAYRALLMNLALAGETDEAKHALQTLRRLAPGMSQNWISQNAVWASADTMK
                     RYVEAFRIAGLK"
BASE COUNT          582 a          992 c         1025 g          633 t
ORIGIN      
        1 caacccgccg gtcctgctgt tgcgcaacaa gagatcggtc tcgccgggca cgccgctgaa
       61 attgccgacg cccgagaact gccaatcgag gccgatggtg ccgatgaagg cggcgccggt
      121 gatcgcattg ttggcgatgt catagacctc caatccgccg gtattggcgt tgcgcagcag
      181 catgtcggat tcgccggcac cgctgaaatt gccgatgccc gagaactgcc agttcaggcc
      241 gacggtgccc atgaaggccg cgccggtgat ttgattgttc gcgagatcgt agacctcgac
      301 cccgccggtg ctcgagttgc gcaggatcat gtcattttcg ccgcggctcg aaaaattgcc
      361 gaagcccatg acctgcctat tcaacccaac cgcaccgagc gaggcggcgt tcgtgatgtt
      421 gttgttgctg acgtcgtaga cctcgaatgc cccggtcctg ccgttgcgca gcaacaggtc
      481 ggtcgtgtcg ctgccgaaaa agccgccgac cgtcacatag ccccaatcgg ttccgacctg
      541 acccaacctg cagccgccca ggatcgcatt gttgccgatg tcatagatct cgtacgtgcc
      601 gctcgtgttg cgcaggatca tgtcggccgt cgtcccggcg ggggggcgct ggatttgggg
      661 ctggcggcgg cggcggagca gtcagcaccg tgctgaattc caggatctcc gcgctgctgg
      721 gatagcttgc ggactcgtgg agaatgaggc cggtggatgt ttgataaatg attacactgc
      781 tggcagtggg gcctgagaac gacaagacgg tcgcattcac tccgtcatat gtttcggttc
      841 cagccacacc atacgtttcg ccttccggac cgcgaatcga cgcgatcgcg gccgccgtgc
      901 tcgtcggatc cacccagaac tggaatccaa atccacttgc gcttccgggt acgaacgtgt
      961 cctgtcctgc ctggtattct atattgccgg tgcttcgggc ggagtatgcg acggtgaccg
     1021 atcccggtga gaccgtcagc gcgtccagga tccaagtccc agtcacggcg ctgttctgga
     1081 tcgaggtgct aaaggcagta gtgtaaacca gagtgaaccc cggcacgacc cagttgggta
     1141 tggtcatcgc ctgctctcct tcgctgccat tcggtaaggt gcgattcggt acggtgcgac
     1201 cgtcgggggg atggccccca aggcgctacg gatcgatctc gattttataa gtgcgcgctg
     1261 gctcaagcgt gctgaggcga tgggagccgg tgtcgaagtc gttcgatcaa gtgagccatg
     1321 ttgttcttgc catctggttt tcttgcgcga agcagtcgcg tcgctgtgat cccagccgga
     1381 atcgaggcta accgaaaacg cgcgcgcgta ccttaaggcc agctaaaggc cggcttgaga
     1441 tttcgataat ttcaactatt gtttgcgcgc cggcgcatgc tcaattcgga cgaacattga
     1501 cgtgatttac cggtttgatg actgcgccct cgataccgat cggcgtgaat tgcgtcgcgg
     1561 cggcgtgccg tgtccgctcg aaccgcaggt gttcgatctt ctggaattcc tgatccgcaa
     1621 tcgggatcgc gtcgtcagca aggaagacgt gttcaactcg gtctggcgcg gccgcatcgt
     1681 gtccgaggcg gtgctgcaca cgcagctgag cgcggtgcgg cgcgcgatcg gcgacagcgg
     1741 ctctgggcag cgcctcgtcc ggacgctgcg taccaagggg tttcgctttg tcggcgcggt
     1801 tcgcgaggaa acgcggacca aaaggacgac gcgcctcgcc gaggaaagcc gcaatcgcgc
     1861 cttgctggcc gatcatccga cgatcgcggt tctccccttt gccaatatca gcggcgatcc
     1921 cgccctggaa tcgctcgccg acggcatgac agaggatctc attacggcgc tctccaaagt
     1981 cggctggctc tacgtggcga cgcgcgcctc ttctttcgcc tgcaagggtc aagtattcgg
     2041 gactgcgcag gccgcccgca agctcggtgt ccgctatctg ctggcgggcg cgcttcgcca
     2101 ggaagtgggt cgccgacgca ttaccgtgca actgatcgac gcgatcgccg atcatcaaat
     2161 ttgggccgaa cgctacgacc gagatttcat cgaccggctc gcagtgcagg acgaaatctg
     2221 cgaacacgtc gtagctgcga tcgagccgca gctctatatc gccgagcagg tccaagttca
     2281 gcgcaagtcg cccgcgaatt tgaacggctg ggaatgtatc gtgcgcgcgt tgtcattgat
     2341 gaacagccgc gaccggaaaa atgtcgcgct tgcgcaggcg cttctgcgaa aggccgtttc
     2401 gatcgatccc aactcggcgc agggtcacag cttgctgtcg attgcgacca cgctgcgcgt
     2461 gcatatgagc tgggcggatc gccagaccgt cgtcccgctc gctctggcgt ctgcgcacaa
     2521 agcgcttgca ctcaacccgg acgagccctg ggcgcacgcc gcgctcggct acgccttgat
     2581 atggaagcgg ccggaggacg cgattgtgcc gtgtcagagc gccgtgaagc tcgatccaga
     2641 cttcgccgcc ggccactact tcctggcgct cggctcggcc tatgccggtt gccacgatcg
     2701 tgtctttccg catgccgcga tggcggaacg cttggcgcgt cgggatttgc tggcgcgcgc
     2761 ctatgccggc gcgcaccaga acgtgcgggc aaccggctcg ttcgcgatcg agcgttacag
     2821 tgagggcgcg gagttcgccc gcagcgccgc cgtgtatatt ccaaattcgc cgacagctta
     2881 tcgtgcgctg ctgatgaatc ttgccctcgc cggagaaacc gatgaggcca agcacgcgct
     2941 gcagacgctg agacgcctgg ctccggggat gtcgcagaat tggatcagcc aaaacgccgt
     3001 gtgggcctcc gccgacacaa tgaaacgcta tgtagaggcg tttcgcattg cgggcctcaa
     3061 gtaaaaagcg acgacgcgtg cacgtcttcg acgccgagcg tctgcgcacc tacgctgggc
     3121 gcgccgcccg cgcgcgccac gccatgacgg cgacgccgac gagaaggacg gcgtcgaggg
     3181 cgagagcgct caggtaggcg gcgtcgaagg cggcgtacac ggcctcggtc aa
//
LOCUS       DATLJT010001463         2913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3347127,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001463 DATLJT010000000
VERSION     DATLJT010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347127"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VM910_33600"
     CDS             <1..295
                     /locus_tag="VM910_33600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI67479.1"
                     /translation="KGKGVNTIAIVSVNDAFVMNAWKRDTDQRDEATFLADGNAEFTK
                     AIGMELDASANGLGIRSKRYSMLVDDGVVKKLNLEPAPGKVEVSGGDALLAQL"
     gene            complement(463..927)
                     /gene="rnhA"
                     /locus_tag="VM910_33605"
     CDS             complement(463..927)
                     /gene="rnhA"
                     /locus_tag="VM910_33605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HVI67480.1"
                     /translation="MSALPLVTIFTDGACSGNPGPGGWGAILKFGDVEKELKGGEPHT
                     TNNRMELMAAISALEALKKPCRVDLYTDSQYVRQGITGWIHGWKRNGWRTADKKPVKN
                     AELWQRLDEALKPHEVHWHWVRGHAGHAENERADQLARDGLAMARLRERVEK"
     gene            complement(924..1901)
                     /locus_tag="VM910_33610"
     CDS             complement(924..1901)
                     /locus_tag="VM910_33610"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HVI67481.1"
                     /translation="MAVYTDVAADELAEFLSQYDIGELLSYKGIAEGVENSNFLVHTS
                     NGAYILTLYEKRVGIDDLPYFLSLMAHLAERGVSCPQPAKTRKGEVYATLAGRPAAVI
                     NFLEGMWPRRPNATHCSGVGQALARMHLAGADFPLYRANPLSVAGWRPLFNLAASRAD
                     SVQAGLSDFIARELDQLEASWPADLPVGVIHADLFPDNVFFLGNRVSGLIDFPFSCND
                     ILAYDIAICLNAWCFETDHSFNVTKARALLNAYGRERKLSAAEESALPLLARGAALRF
                     LLTRLVDFLNVPPGALVKPKDPLEYARKLRFHQNVASVRDYGLAAGVVA"
     gene            complement(1911..2873)
                     /gene="ispH"
                     /locus_tag="VM910_33615"
     CDS             complement(1911..2873)
                     /gene="ispH"
                     /locus_tag="VM910_33615"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HVI67482.1"
                     /translation="MAAKPDLRIVLCSPRGFCAGVVRAIDTVERALTIYGAPVYVRHE
                     IVHNKYVVDGLKKKGAIFVEELSEIPDNTNAPVVFSAHGVPKSVPAEARSRNFFSLDA
                     TCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPVGAVTLIETAHDAQTFA
                     PRDPNNLAFVTQTTLSIDDTADIVAILKGRFPNISGPHKEDICYATTNRQLAVKKVAP
                     VVDALIVVGAPNSSNSQRLREVAEREGCPIAVLAQRAGDIDWERFEGIRSLGITAGAS
                     APEVIVEEIMGAFAERFQLHVETVSAAEENEFFPLPRSVRPDAA"
BASE COUNT          538 a          930 c          923 g          522 t
ORIGIN      
        1 caagggcaag ggcgtgaaca cgatcgcgat cgtttcggtc aacgacgcct tcgtcatgaa
       61 cgcctggaag cgcgacaccg accagcgcga cgaggccacc ttcctcgccg acggcaacgc
      121 cgagttcacc aaggcgatcg gcatggagct cgatgcctcc gccaacgggc tcggcatccg
      181 ttccaagcgt tattccatgc tggtcgacga cggcgtggtg aagaagctca acctcgagcc
      241 tgcgcccggc aaagtcgagg tttcgggcgg cgatgcgctg ctcgcgcagc tctgagccga
      301 ccattctccg ctgctgtgcc cggcttgccg ccttcgcttc ggcgtgcccc gccgaagcct
      361 tggcgtaggc gggaccgggc aacccagcac tgaaatctcc gggatactgt gtcgcccgct
      421 ttcgcgggcg atgacagggg agtgtgtgga cgacggctcc catcacttct ccacccgctc
      481 ccgcaaccgc gccatcgcga gaccgtcgcg tgccagttga tcggcgcgct cgttctcggc
      541 gtgcccggcg tgacccctga cccagtgcca gtgtacctcg tgcggcttta gcgcttcatc
      601 caaccgttgc cacagttcgg cgttcttgac cggcttcttg tcggcggtgc gccaaccgtt
      661 ccgcttccag ccgtggatcc agccagtgat gccctgacgc acatactgac tgtcggtata
      721 gaggtcgacc ctgcacggct ttttcagtgc ttccagcgcc gaaatcgcgg ccatcagttc
      781 catacgattg ttggtggtgt ggggctcgcc gcctttcaat tctttttcga cgtcgccgaa
      841 tttcaggatt gcgccccagc cgccgggccc gggattgccc gagcaggcgc cgtcggtgaa
      901 gatcgtgaca agcggcaggg cgctcacgca accacaccgg cagcaagacc gtagtcgcgc
      961 acgctcgcga cattctggtg aaagcgcagc ttgcgggcat attcgagcgg gtccttcggc
     1021 ttcaccagcg cccccggcgg tacattgaga aaatcgacca gccgcgtcag gaggaagcgc
     1081 aaggcggcgc cgcgtgcaag cagcggcagc gcgctctcct cggcagccga taatttgcgc
     1141 tcgcggccat aggcgttgag cagcgcgcgc gccttggtga cgttgaagga atgatccgtc
     1201 tcgaagcacc acgcattgag gcagatcgca atgtcgtagg cgagaatgtc gttgcaggag
     1261 aacgggaagt cgatcaggcc cgacaccctg ttgccgagaa agaagacatt gtcgggaaac
     1321 aggtcggcat ggatgacgcc gaccggcagg tcggccggcc agcttgcttc gagctgatcg
     1381 agttcgcgcg cgatgaagtc ggagagcccc gcctgcacgc tgtcggcgcg cgaagcggca
     1441 agattgaaca gtggccgcca gccggcaacc gacagcggat tcgcgcggta cagcggaaaa
     1501 tccgcgcccg ccagatgcat cctcgcgagc gcctggccaa cgcccgagca atgggtcgcg
     1561 ttcggtcgcc gcggccacat gccctcgaga aaattgatga ccgcggccgg ccgccccgcc
     1621 agcgtcgcat agacctcgcc cttgcgggtc ttggccggct gcgggcagga gacgccgcgc
     1681 tcggcaagat gcgccatcag cgacagaaaa tacggcagat cgtcgatgcc gacgcgcttt
     1741 tcatagagcg tgaggatgta ggcgccgttc gaggtgtgca ccaggaagtt ggaattctcc
     1801 acgccctcgg cgatgccctt gtacgacagg agttcgccga tatcgtattg ggagaggaat
     1861 tccgcaagct cgtcggcggc tacgtcggta tagaccgcca tgacaagtct ctacgccgcg
     1921 tcgggtcgca ctgaacgcgg cagcgggaag aactcgtttt cctcggcggc cgagaccgtc
     1981 tccacatgca gttgaaagcg ctcggcgaat gcgcccatga tctcctcgac aatcacttcc
     2041 ggcgcagacg cccccgcggt gatgccaagg ctcctgatgc cttcgaaacg ctcccagtcg
     2101 atatcgccgg cgcgctgcgc cagcacggca atggggcagc cctcgcgttc ggcgacctcg
     2161 cgcagccgct gcgagttcga ggagttgggc gcaccgacca cgatcagcgc gtcgaccacc
     2221 ggcgccacct tcttcaccgc aagctggcgg ttggtggtgg cgtagcaaat gtcttccttg
     2281 tgcggcccgg aaatgttcgg gaaacgcccc ttgaggatcg caacgatatc ggcggtgtcg
     2341 tcgatcgaga gcgtggtttg cgtgacgaag gcaagattgt tgggatccct cggcgcaaac
     2401 gtctgggcat cgtgggcagt ctcgatcagc gtcactgccc ccaccggcaa ttgcccgagc
     2461 gttcccacca cctcggggtg atgggaatgg ccgatcagca ggatctcgcg gccgcgcttg
     2521 aaatggatgg ccgcctcccg gtgcaccttg gtgaccagag gacaggtggc gtcgagcgag
     2581 aagaaattgc gggagcgggc ctcggccgga accgatttcg gaaccccgtg ggcggaaaat
     2641 accaccggcg cgttggtatt atcggggatt tccgaaagtt cctccacgaa aatggcgccc
     2701 ttctttttca agccgtccac gacatatttg ttgtgaacga tctcgtgtcg gacatagaca
     2761 ggggcaccat agatggttag cgcccgttcc acagtgtcga tggcccgtac caccccggcg
     2821 caaaagcccc gcggcgaaca aagcacgatt ctgaggtctg gttttgcggc cataggagcg
     2881 atctctggac cgaatcaccc ctcgccggcg ggc
//
LOCUS       DATLJT010001464         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3119290,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001464 DATLJT010000000
VERSION     DATLJT010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119290"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VM910_33620"
     CDS             <1..915
                     /locus_tag="VM910_33620"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HVI67483.1"
                     /translation="GTYSDAFTSAGISGVKIEMTEERGWTRSQSHGHRMARRIPTVVG
                     RIAWKGPQNADDFRFLKSLTTRTPKITLPGPCYIHYRAGRANISRDVYPDLDAFWSDL
                     VEAYVQEMRSLADAGCRYLQIDETSLVKLGDPRVRQLLAERGDDWRDLLRVYVDVVNA
                     VVAGAPEGMTIGIHVCRSQDPSWQADVGYDPIAEPLFNAMKVDTYFLEYDNARAGTFA
                     PLRCVPKEKFVVLGLVASKVSELESVDVLKRRIEEASRYVKLEQLGLSPQCGFATSAA
                     EHVTVTEEIERAKLARIVEVARQVWGSV"
     gene            complement(945..1619)
                     /locus_tag="VM910_33625"
     CDS             complement(945..1619)
                     /locus_tag="VM910_33625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HVI67484.1"
                     /translation="MSSTKLFSRQAWDKNASIYEVIRTMPFNAQLASGTLSEARFKHY
                     ITQDAHYLIGFGRALTLAAAKAPNPDRIVQFAKAAEGALVVERALHGSFFKQYGITPR
                     AFAQTPLSPACHHYVSYLLATAYADPYEVLLGALLPCFWIYAEVGRDIHARARAANPY
                     QAWIDTYAGEEFHAAVRAVIAATDEAAADVSPSLRARMHAAFTRATQLEWAFWDSAFR
                     LETWPV"
     gene            2808..3389
                     /locus_tag="VM910_33630"
     CDS             2808..3389
                     /locus_tag="VM910_33630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI67485.1"
                     /translation="MTVYGYARVSTNGQTLASQDAELHAAGAAKVYAEKMSGARTDRP
                     ELGKLLKRLDAGDVLLVTRLDRLARSTRDLLNILDLIGKRDAGFKSLADPWADTTTPH
                     GRLMLTVLGGLAEFERALILARTSDGRTRAKAKGVKFGRPAKLTAHQRREAMQRLAEG
                     AVQADLARSYGVSQATISRLAAPGPFAASAVVV"
BASE COUNT          792 a         1246 c         1373 g          762 t
ORIGIN      
        1 ggcacctatt ccgatgcctt caccagcgcc ggcatttccg gcgtgaagat cgaaatgacg
       61 gaggagcgcg gctggaccag gtcgcaaagc cacggccatc ggatggcgcg gcgcattcca
      121 acggttgtcg gccgcatcgc ctggaaggga ccacagaacg ccgacgactt tcgctttctc
      181 aagtcgctga cgacgcgcac gcccaagatc acgctcccgg gcccctgcta cattcattat
      241 cgcgcggggc gcgccaatat cagccgcgat gtctatcccg acctcgacgc tttctggtcc
      301 gacctggtcg aagcctacgt ccaagaaatg cgctcgctcg cggacgccgg ctgccgctat
      361 ctgcagatcg acgaaacgtc gctggtgaag ctcggcgatc cgcgcgtgcg tcagttgttg
      421 gcggagcgcg gcgatgactg gcgcgatctc ctgcgcgttt acgtcgatgt cgtcaacgcc
      481 gtcgtcgcgg gcgcgccgga gggaatgacc atcgggattc acgtttgccg cagccaggat
      541 ccgagctggc aagccgacgt cggttatgac cccatcgcgg agccgctgtt taacgcaatg
      601 aaggtcgaca cctattttct cgaatacgac aatgcgcgcg ccggcacatt cgcaccgctg
      661 cgatgcgtac cgaaggaaaa attcgtggtg ctcggcctcg tcgcctccaa ggtttccgaa
      721 ctggagagcg tcgacgtcct caagcggcgg atcgaggagg caagccgcta cgtcaagctc
      781 gagcaactcg gcctgagccc gcaatgcggg ttcgcgacta gcgccgccga gcacgtcacg
      841 gtgaccgaag agatcgaacg ggcaaagctt gcccggatcg tcgaggtggc gcgccaggtt
      901 tggggaagcg tttgatcgac gacgctgtgc caacgacgct ctgactagac cggccaggtc
      961 tccaatcgga aggccgaatc ccagaacgcc cactcgagct gggtcgcgcg cgtgaaggca
     1021 gcatgcatcc gcgcgcgcag ggatggcgag acgtcggcgg cggcttcgtc cgtggcggcg
     1081 atgacggcgc gcactgcggc gtgaaactcc tcgccggcat aggtgtcgat ccaggcctga
     1141 tacggattgg ccgctcgggc gcgggcgtgg atgtcgcgcc ccacctccgc atagatccaa
     1201 aagcagggca gcagcgcccc gagcagcact tcgtatgggt ccgcataggc ggtggcgagc
     1261 agatacgaaa cgtaatggtg acacgccggc gagagcgggg tctgcgcaaa cgcgcgcggc
     1321 gtgatgccgt actgcttgaa aaacgagccg tgcagcgccc gctcgacgac cagcgccccc
     1381 tccgctgctt tggcgaactg cacgatccga tccggattcg gcgccttcgc ggccgcgagc
     1441 gtcaaagctc gtccaaagcc gatgaggtag tgcgcgtctt gcgtgatgta atgcttgaag
     1501 cgcgcctcgc tcaacgtgcc ggacgcgagc tgcgcgttga agggcatcgt gcggatgacc
     1561 tcgtagatgg aggcgttttt atcccaggct tggcgcgaga agagtttcgt gctgctcatg
     1621 gtcgagggtg ttcctggaac caaacgacga actccacgac gtacggaaac atggtgggcc
     1681 cgggagtagt gacacacgct aatctggtca atggcttgcg tgtcaaacag ctccaaaagg
     1741 cccctttgaa cgcaaacggg tttctcagca agtgtcaaaa ttggagcgat acgtttgtcg
     1801 ggggacgttg tcgccaatct tgttcaaggg gatgccccta tgactgacgc cagcttcgcc
     1861 gaacacctca agtttctgcg cgggttcaga aaagagcgcg gctatgaccc tcatggacta
     1921 agagaggcac agatccagcg gcgcgccttg ggcggcttgt cgacggcact ggaggccacc
     1981 tgggggtggc gtgggcgcat ctccgatgaa ggaggagcag ggcaagctgc cgtggagcaa
     2041 gcccaccctg gtggctgtgc caagggagaa ctatgacccc acgagtcttc gcgctttcga
     2101 cccgcaagcc gctaggacag ccaagcggtt ttgtcggctc gccggtggca ggcggctcgg
     2161 aatggcaata aacggaggca atggcagcga tgttttatcc gggcgaagga aaaaagggcc
     2221 agcccaaggc gggcgattac gtcttggccg cagagcaaac gagagcggaa gcggcggcta
     2281 gtccgcaccg aaacacggcg gttgatttag cacgcgcgga caagtgggag gggcaagcgc
     2341 gcacaaaacc actgccgtcc actcgaaccg aaatcggagc gggtcgcgag ctgatagacg
     2401 ttgaaaacag gttcgcgctt ctgacactga aatatggccc acgtctgtaa cctgtccccg
     2461 tccgccgacg ccgtccctcg atcgcggccc ggttcagcgt cgggttcggc gcgcgttcat
     2521 cgccacgggc aagccggtgc tatcgacaag ccagatcgtc gagtggtcac atcctcgccc
     2581 cggcaccgat tggcaccatg cgcggcgttc tgtccgtcgt gtctgtgagc gctactgcgt
     2641 ccgcgtcggc cgcagcgaca gcggtagcgg tcggccgatc ctgtggcgat tgcgcaacag
     2701 cggggactag tttcatgcaa aactcacaag ttatgcatag aaatattgaa actgatttcc
     2761 cgttgtgatt tttatgcata tgttgcttta gaaagtggag gctcgaaatg acggtttacg
     2821 gctatgcgcg cgtcagcacc aacggccaga ccctggccag ccaggacgcc gagctgcacg
     2881 ccgcgggtgc agccaaggtt tacgccgaga agatgagtgg cgctcggaca gatcggcctg
     2941 agctgggcaa gctcctcaag cgcctcgacg ccggcgatgt gctgctggtg acgcggctcg
     3001 atcggctcgc tcgatctacg cgcgatctgc tcaacatcct cgatctgatc ggcaagcgcg
     3061 acgccggctt caagtcgctc gctgatcctt gggccgatac gacgacgcca cacggccggc
     3121 tgatgctaac cgtgcttggc ggtcttgctg agttcgagcg tgcgctgatc ctcgctcgca
     3181 cgagtgatgg cagaacacga gcgaaggcca agggtgtgaa gttcggccgt cctgccaagc
     3241 tcactgcgca tcagcgacgg gaagcgatgc agcggcttgc cgagggtgca gtgcaggctg
     3301 atcttgctcg ttcgtatggt gtgagccaag cgaccatcag caggctggca gctcctggcc
     3361 ctttcgcggc aagcgcggtc gtcgtgtgaa acgccgccgc gattaagttt ctgagaaatc
     3421 gagacccccg ggagggtgga ggttaaaacg gtgccatcga ggagtcccat tgtgcttggt
     3481 cctccccatc ccctgtgtgg actcgcgccg cacaagcggg gccgcaaggc cgaaaccggt
     3541 gcattcgtca tccgacgccc tgtcggtcgc cgtgtgggga gggtatgaat gggcgcaggt
     3601 tcccggcttg ctcgctgggc cgggcatcga cagaggtcac tgggggtcaa gggcgaaggc
     3661 tatggctcaa accgttcgcg agaaggcccg aatacgtcaa gcccgcaaag atggtcggcc
     3721 gagcgaggac gacatcgcgc aggaccgcct cggctggcgc ggtcaaaagg gccggccgag
     3781 taccgcgacg atgacggagg acagcaggtt gcaaacccca ttccacctcg atcccggcca
     3841 tacggcttga tgtcggcgga tgcgccgggt cgcgtccgtg tgggctgttt tctgcgcgct
     3901 tggtgtggag tgttttctgc gcgggcggcc gtgcgatcga ggaccggggc caggatgtcg
     3961 attgctgact cagcggcggc cggtggtgcg tttttttacc tcgtcggccg tcgagaggga
     4021 ttcgccgaac gggatttatg gaaagtctgc ccgatcttcg gtcgagactt catccctgcg
     4081 gcttgatgtt ggccgcccgg atcaccttgc cccatgtttc gagttccgtc gcgattcgcc
     4141 gcgcgaattc ctctggcgtg cttccgaccg ggt
//
LOCUS       DATLJT010001465         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2888385,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001465 DATLJT010000000
VERSION     DATLJT010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2888385"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..506)
                     /locus_tag="VM910_33635"
     CDS             complement(267..506)
                     /locus_tag="VM910_33635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67486.1"
                     /translation="MSRAYLSRKLTRPLPTKDGGTLRTVLDARAYMFRLSKDWERSAR
                     WQRAAQLLLAKANVGAVSRQLELALVHDGKLDLAQ"
     gene            complement(718..1092)
                     /locus_tag="VM910_33640"
     CDS             complement(718..1092)
                     /locus_tag="VM910_33640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67487.1"
                     /translation="MVGGIMDLAGMPRRVADYPDAFADWNYISSIGSYISALGLIAFG
                     VVASSARMVASFRAFIPAPSIHATNGGKPQHMAPLTGRPEPACSSCCGGCRLCLPDGC
                     QWCRSLRGRLDFGFGELAAQVC"
     gene            complement(1639..>2880)
                     /locus_tag="VM910_33645"
     CDS             complement(1639..>2880)
                     /locus_tag="VM910_33645"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI67488.1"
                     /translation="LLRRYWHPIAASDEMTSRPTKAVRILGETLVLFRDALGNFGLIG
                     DRCPHRRASLLFGMPDGDGLRCAYHGWRFDRAGQCTDMPGEPPRAAEKRRVSITSYPV
                     EELGGLIFAYLGPGPTPRLPRWELLAESGLPRDIVTIDVPCNWLQIMENSIDRLHFEW
                     LHIRFHNFALAQSGQEPRADPSREIASHRLADTYEETEYGFAKRTSFRRPDGLERSAI
                     LPFVFPTGGQTGLPRAPCLQLRTPIDDDNTRQYFYTLYDLEIEMKARLRSQLGDEILD
                     GKHVQHLQIPPPPFGEPNYSLLLSNVYQDVSIVTSQGRIHDRTQEQPGSSDKGVMLYR
                     SLLRRELAKLSDGKDPINVFRGPFPNDRIELPRRSKDGVSSLYPRAAGRPSGNVQLWS
                     PFLSQIERLGIDLNEVLFGAG"
BASE COUNT          550 a          887 c          896 g          547 t
ORIGIN      
        1 gggcgaccga atgcacgaat tcgacgctcg caccgatgga gaagttcgat tcctcgggca
       61 agtttcataa ggcgatcggc gctgacaggt tcgcggttcc gcacggtttc tatgtggatc
      121 gcgagggcgg tgtgtggggc aggtgtctag gaggctaaac agttcctcat ggacctaaag
      181 ccccggcacg tacgggcttt ctttaactca gcctcgcggc gtcttcggcg gcagcttggc
      241 cgaggcccgt ttgagtagca gcaccgtcat tgcgcaaggt cgagcttgcc gtcatggacc
      301 aacgcgagtt cgagttgcct gctgacagcg ccgacattgg ccttggcaag gagtaattgg
      361 gccgcccttt gccaccgggc gcttcgctcc caatccttcg acagccgaaa catgtaggcg
      421 cgggcatcta ggaccgtgcg cagcgtgccg ccgtctttgg tcgggagggg gcgggtcagc
      481 tttcgggaga ggtatgcccg tgacacggcc ggaagcataa gcgccccggg aggggaatgc
      541 gagttagcgg cgtgctgctg gcgcggcggg tcggggctcg gcctcgtcaa cacagccgtt
      601 gctgatgatc cgacattgcg tcccggggat atcgtcgcga ccaatactgg tctgatggcc
      661 tatagcgccg ggccaaacgg ctcgaccttt atgcccatca gcgcctatgc gggcctctca
      721 gcagacttgc gccgccagct caccgaaacc aaaatcgagc cggccgcgga gggacctgca
      781 ccactggcac ccgtccggca ggcagaggcg acatccgccg cagcacgaag aacaagcggg
      841 ctcaggtcga ccggtaagcg gcgccatgtg ctgaggcttc ccgccgtttg tcgcgtggat
      901 gctgggtgcg ggaatgaatg cgcgaaagct cgcgaccatc cgtgcgctgc ttgccaccac
      961 gccgaatgcg atcaggccca aggccgagat gtaggatccg atcgacgaga tgtagttcca
     1021 gtcagcaaaa gcatcgggat aatctgcaac ccgtcgcggc attccggcca aatccatgat
     1081 gccgccgacc atggcgccga agtcgtttcg ttccattcgc ggagaggcgc gggaagccga
     1141 ggcgcgcgct gtgttgaggc gcgggcggcg gctattcgtt aggttctctg acgaccgccg
     1201 gctctcctgc ctctcgtcgt cgtcgtgctc gtcgtcctcg tcgtcgtcct tgtcgtcctt
     1261 gtcgtcttcg tacgcctcgc agtcagcgcg gccgaaacgt cagcccgacg cggtgagatt
     1321 cgctcgctcg attgcgttat agcgaagccg ccaagcatgt cgccaaggcg gctggcgtgt
     1381 ttcctccgaa cggcgcttac gaggacaagc ggattagcta cctcctgata aggtgtcgcg
     1441 tcctcgcggt caaaattgcg aacgaactcc gtaacaagag gcctgtttga aaggcatccg
     1501 cccacttgat ggcggcagct tcggatcagc gcataggacc ggctgtggct cccagcccaa
     1561 acgataaaac tcgacagcga aagctctgtt gtaagccgat tggtgttcgt gtcgcccaca
     1621 ccttcgtcag ggcggtggct agccagctcc gaaaagcact tcattcagat cgattccaag
     1681 acgctcgatt tgcgacagaa acggactcca cagctgcacg tttccggacg gccgacctgc
     1741 ggcgcgcgga tagaggctgc tgacgccgtc cttggacctg cgcggtagct cgatacggtc
     1801 gttcgggaat ggcccgcgaa agacgttaat cggatctttt ccatcgctga gcttggccag
     1861 ttcgcgccgg agcaagctgc gatacagcat gacgcctttg tccgaacttc cgggctgctc
     1921 ctgagtgcga tcatgaatgc gtccctggct cgtcacgatc gagacgtcct gatacacgtt
     1981 ggacaacagc aacgaataat tgggctcgcc gaaaggcgga ggcggaatct gcaaatgctg
     2041 gacatgtttc ccgtcgagga tttcgtctcc gagctgcgat cgcagccgcg ccttcatctc
     2101 aatttccaga tcgtagagcg tgtagaaata ctgccgcgtg ttgtcatcat caatcggcgt
     2161 tcgcagctga agacacggag cccgcggcag ccccgtctgc ccacccgtcg gaaacacaaa
     2221 aggcagaatc gcggagcgtt ctaacccgtc gggtcgtcga aaagacgtgc gcttggcgaa
     2281 gccgtactcc gtctcctcat aagtatccgc gagccggtgc gatgcgatct cgcgactggg
     2341 gtccgcgcgc ggctcctgtc cgctctgcgc gagcgcgaag ttgtgaaagc gaatgtgcag
     2401 ccattcaaag tgcagccggt caatggagtt ctccatgatc tgcagccaat tgcacggcac
     2461 atcgatggtg acgatatccc tcggcaagcc cgactctgcg agcagttccc acctcggtag
     2521 ccgcggcgtc ggcccgggcc cgaggtaggc gaagatcagt cctccaagct cctcgaccgg
     2581 atagctcgtg atcgaaacgc ggcgcttctc cgcggcgcgc ggcggctcgc ccggcatatc
     2641 cgtacactga ccagcgcgat cgaagcgcca cccgtgatag gcacagcgca gtccatcgcc
     2701 atccggcatt ccaaacagca gcgatgcgcg ccgatgcgga cagcgatctc cgatcagacc
     2761 gaagtttcca agcgcgtcgc gaaacagcac cagggtctca cccagtatgc gcacggcctt
     2821 ggtcggccgg gacgtcatct cgtcgctggc ggcgattggg tgccagtagc gacgcagcag
//
LOCUS       DATLJT010001466         1758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4266821,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001466 DATLJT010000000
VERSION     DATLJT010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266821"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VM910_33650"
     CDS             complement(<1..597)
                     /locus_tag="VM910_33650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67489.1"
                     /translation="MSIASTSAATSTPYYLPVRQDWLDRRKEPILGPDLPIVDPHHHL
                     WDRPGWRYLLDELLADTASGHNIVATVFVQARAMTRAAGPVEMRPVGETEFVNGVAAM
                     SVSGVYGKTRHCAGIVGHADLTLGSRVEPVLAAHIRAGGDRFRGIRHISAWDADASIR
                     NPAYSPPRGLLGDKNFREGFAVLGRLGLSFDAWLYHPQI"
     gene            702..1049
                     /locus_tag="VM910_33655"
     CDS             702..1049
                     /locus_tag="VM910_33655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67490.1"
                     /translation="MPISSKYLFVVTMDVDADKEALFNEVYDTEHIPNLLKVPGVRAV
                     TRMAGEPFALSIGGAEKKVPHDGPRYSAIYEIDGPQVLVSPAWAKAVEEGRWPGQVRP
                     FTHNRRHALYKVR"
     gene            1243..>1758
                     /locus_tag="VM910_33660"
     CDS             1243..>1758
                     /locus_tag="VM910_33660"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HVI67491.1"
                     /translation="MRRQILPSLLVGAAMLVSSELCAQQPAAPNLDAVPEKMPFSTAY
                     GSPIGAKRANAVIEAAAAEASKKGWPMNIAVVDSGANLVAFLRMDDAQLASIAIAEHK
                     ARAAAKYRRPTKVFEDVVQKFDLKYILSLDDVVASRGGIPLIEDGKIIGAIGCSGGTG
                     SQDEVICTAGAA"
BASE COUNT          326 a          541 c          596 g          295 t
ORIGIN      
        1 gatctgggga tggtagagcc aggcgtcgaa ggagaggccg aggcggccga gaacggcgaa
       61 cccttcgcga aagtttttgt cgcccaagag accgcgcggc gggctgtagg ccggattgcg
      121 gatcgacgcg tcggcgtccc aggcactgat gtggcggatg ccgcggaagc gatcgccgcc
      181 ggcgcgaatg tgggcggcga gcaccggctc gactcggctg cctaacgtca ggtccgcgtg
      241 gccgacaatg ccggcgcaat gcctggtctt gccgtagacg ccgctcacgc tcatggcggc
      301 gacgccgttg acgaactcgg tttcgcccac ggggcgcatt tcgaccgggc ctgcggcgcg
      361 cgtcatcgcc cgcgcctgca cgaacacggt ggcgacgatg ttgtggccgc tcgccgtatc
      421 cgccaacaat tcatcgagca gataacgcca gccgggccgg tcccacagat ggtggtgagg
      481 atcgacgatg ggcagatcgg gcccgaggat cggctccttg cgccgatcga gccaatcctg
      541 gcgcacgggc agatagtagg gggtcgacgt tgcggccgac gttgaggcga tcgacacggc
      601 gggttccctc caggcggtag catcgcagca gcacgtcacc tacgatagca cgatccccgt
      661 gcgggcgtga cgcaagcgca agataagcga ggcaggtcct catgccgatc agctccaagt
      721 acctgtttgt agtgaccatg gatgtcgatg ccgacaaaga ggcgctcttc aacgaggtct
      781 acgacaccga gcacatcccc aaccttctca aggtgccggg cgtgcgcgca gtgacgcgga
      841 tggcaggaga gcccttcgcc ctgagcatcg gcggcgccga gaagaaggtc ccgcatgacg
      901 gcccgcgcta cagcgcgatt tacgagattg acggccccca ggtgcttgtc agtccggcgt
      961 gggccaaggc ggtagaagaa ggccgctggc cgggccaggt gcggcccttc acgcacaatc
     1021 gccgacacgc gctttacaag gtgcgctagc gcattgccgt cgaaagccct cgtggctcaa
     1081 aactgtcgcg gtagccctgc cgaattctgg atcgcgcgga ggctgcgctt gcgggttgag
     1141 gcgtaagggc cggttctata aagtcactcg cgcgctgctt tcaacggcgc ggtcaacgct
     1201 catgcgatag tggtcttcaa cgacagtctt gggaggttat ccatgcgaag gcagattttg
     1261 ccgtcactgc ttgtcggtgc ggcaatgctg gtcagttccg agttgtgcgc ccagcaaccc
     1321 gctgcgccca atctcgacgc cgttcctgaa aagatgccgt tcagcaccgc ctatggatcg
     1381 ccgattggag cgaaacgcgc caacgcggtg atcgaggccg ccgccgcgga agccagcaag
     1441 aagggctggc cgatgaacat cgcggtcgtc gactcgggcg ccaatcttgt cgccttcctg
     1501 cgcatggacg atgcgcaact cgcctcgatc gcaatcgccg agcacaaggc gcgcgccgcg
     1561 gccaagtacc gccgtccgac caaggtgttc gaggacgtgg tgcaaaagtt cgatctcaaa
     1621 tacatcttga gcctggatga cgtcgtcgcg tcgcggggcg gcatcccgtt gatcgaggac
     1681 ggcaagatca tcggcgccat cggttgttcg ggcggcaccg ggtcgcagga cgaggtgatc
     1741 tgcacggcag gtgcggcg
//
LOCUS       DATLJT010001467         2033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2429313,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001467 DATLJT010000000
VERSION     DATLJT010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2429313"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VM910_33665"
     CDS             complement(<1..874)
                     /locus_tag="VM910_33665"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI67492.1"
                     /translation="MNAESAAELSREELIALVAALRAHIAELERRLGLNSSNSGKPPS
                     SDGLKKPARVSSLRERSGKKPGGQKGHKGETLRQVIDPNEVVNHYPPACSTCGVGLDP
                     KTSVGYSARQVFDLPQPQPLVVTEHRAHDCECAACGAKTRALFPDSVNAPVQYGARIT
                     AFVIYLLHYQLLPENRLATLMADLFGVKVATATIARMSRACAERLHGFVETVRDLVAG
                     APVKHMDETGFRIGGKTQWLHVASTAVLTFYRVCAKRGSLLANVVGIVVHDHWKPYYT
                     IEGVLHALCNAHHLR"
     gene            complement(1473..1619)
                     /locus_tag="VM910_33670"
     CDS             complement(1473..1619)
                     /locus_tag="VM910_33670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67493.1"
                     /translation="MIERTPTADDDDTRPVLSGTAKLIIAVIVFAFGMLAGAAIAAWI
                     MPPS"
BASE COUNT          410 a          617 c          593 g          413 t
ORIGIN      
        1 cgcgcagatg atgagcattg cacaaggcgt gcagcacacc ttctatcgtg taatatggct
       61 tccaatgatc gtgaacgaca attccgacga cattcgccaa gagactgccg cgcttggcgc
      121 agacacgata gaaggtcagc acggcggtgg acgcaacatg cagccattgt gtcttgccgc
      181 caatgcgaaa ccccgtctcg tccatgtgct tcaccggcgc gcccgccacg agatcacgca
      241 ccgtctcaac aaaaccatga agacgttcag cgcaagcccg gctcattctc gcgatcgtgg
      301 cggtggccac tttgacaccg aagagatcgg ccatcagtgt ggccaggcgg ttctcgggca
      361 gcaactgata gtgcagtaga tagataacga aggccgtaat tctggcgccg tattgcaccg
      421 gcgcgttgac gctgtctgga aacagcgcgc gcgtctttgc gccacaggcg gcacattcgc
      481 aatcgtgcgc ccgatgttcg gtcacgacga gaggctgcgg ctggggcaga tcaaacacct
      541 gacgggcgct atagccaacg ctcgttttcg gatcgaggcc cacgccgcac gtcgaacagg
      601 ctggcggata atgattgacg acttcgttgg gatcaatgac ctgtcgcagg gtctcgcctt
      661 tgtgaccctt ctgtccaccc ggtttcttgc cggaccgttc gcgcaaactc gacacccgcg
      721 ccggcttctt caacccatcg ctcgacggcg gcttgccgct gttcgaactg ttcaatccga
      781 gccgacgctc aagttcggcg atatgggctc tcagcgccgc aaccagcgca atgagttctt
      841 ctcgcgatag ttctgccgcc gattcggcat tcatcgaatc cttgaatcga agtcagattc
      901 cgctgtcgag aaaattctgc acggttgtct gcgggaaaaa atgcagccta acctggccag
      961 ttacattgta ttaacagtta attggccccg gttttctcct cacggatatg gtcctcaccg
     1021 acgtttttcc gcatcgctgt ggacgcgaac atgtccctcc tctcggcgtg tccgccacgc
     1081 aacaggaagc aggaaaagta gtggcaaagg aatgcatgga ggtcgtttcg cttgccgcgg
     1141 tgcctgtaca gggctaacga tagcgaatgt tgtggcgggg tgtcttcgca gaacttgctg
     1201 tgatcgcggt ggcagccgcc gcgggcgctg ctgccttcct ggttcccact cttgtggtgc
     1261 ccatcttctg atcagtcacg ccggctcatt cagcggccag tgcgccaagg ccaatatcgg
     1321 cgcatctcgc ctccgccaaa gctggctgcc tcgccgactg catacaggcc cgggacgatg
     1381 tcgcggtcgc taccgaacag tctccattca tcccagccac aaggtcttgg ccgggcaagc
     1441 gccacaggta ccgcgtagtg cgttgcctcc actcaggaag gcggcatgat ccacgctgcg
     1501 atggctgcgc cggcaagcat gccaaacgcg aacacgatga ctgcgataat cagcttggcc
     1561 gtaccgctga gtaccggccg ggtgtcgtca tcgtcggctg tgggcgttcg ttcgatcatg
     1621 gggcgccaca atagtcagtt gctcgattat ctttcctagt tttccgtccc agaagagacg
     1681 atggatcgcc cctacaatcg aagtgttgcg gaaacatggc ctgtgtcgat cgggggcact
     1741 tattcagcag gcgttgcaaa tcaaccgcat gcgcgtggta aaaggtccgt ccgaccgatg
     1801 tctttgacgc cgctgctcgc tccgcgaaaa cctccaattg acgaagggca ttgcggctgc
     1861 ggtgatgcgg ccggatgccc ctcggccgcg cgatgccaat tagctgccgg ttggcgggca
     1921 cccgcacaag gcccgggagt atcagcgcag gtggcgggcc gcgaacaaga acaagacccg
     1981 tgaggagagg ctcaccaagc gggccgccgt cagctgcgca aggacaggat caa
//
LOCUS       DATLJT010001468         4932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_818549,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001468 DATLJT010000000
VERSION     DATLJT010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818549"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VM910_33675"
     CDS             <1..138
                     /locus_tag="VM910_33675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVI67494.1"
                     /translation="AANPFERRFRDMHTIAQQIQARDTHYEDAGRAILSARLQKPPTA
                     R"
     gene            293..682
                     /locus_tag="VM910_33680"
     CDS             293..682
                     /locus_tag="VM910_33680"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67495.1"
                     /translation="MPVVGCLSSSNAGFATFLAAFREGPSEQGFVEAKNVRIEYRSVD
                     ESRYDRLPALAKELVEHHVSVIFANPIPAAPAAKAAAGATPVVFAVGSDPVGTGLVSS
                     INRPGGNIIGVSFLTVALAATSIFRRA"
     gene            880..1833
                     /gene="mepA"
                     /locus_tag="VM910_33685"
     CDS             880..1833
                     /gene="mepA"
                     /locus_tag="VM910_33685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HVI67496.1"
                     /translation="MNARLILIPLLMMMLARACPALAQDKGTVDAKPLPPLANPNDPK
                     IGAKQLFARKLLPSSGPPKVIGAYNKGCVAGAAKMPLNGDNWQVMRLSRNRYWGYPGT
                     IALLKRLAANVHKDTGWPGILVGDIGQPRGGPALSGHASHQIGLDADIWLTPMPDHRL
                     SREEREEMSATMMVREDRLDVDPKAWTPQHWMLLRDAAQEPGVQRIFVNAAIKKALCR
                     EAKGDRSWLAKIRPWYGHDYHFHIRMRCPAGSSECKGQSDQGGGEGCNASDLAYWFKD
                     SIIHPKPEPEPKEPPKPKVGITLAQLPKACRAVLNAPDAKR"
     gene            1994..2200
                     /locus_tag="VM910_33690"
     CDS             1994..2200
                     /locus_tag="VM910_33690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007702758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HVI67497.1"
                     /translation="MRISARNQIRGTVIEVKKGATTSHVRVDIGGNVVTSSITNEAVD
                     ELGIKAKDSVIVVIKASDVMIGVD"
     gene            2203..2709
                     /locus_tag="VM910_33695"
     CDS             2203..2709
                     /locus_tag="VM910_33695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67498.1"
                     /translation="MRPPLLIALALLGAAPAFAAEEPSGCDKFKWNIDHERAALTSTD
                     QQMLASGTELTALPARGVILALVAPAEAKLPSPPERGPKDGTLAGFASFKTAPKTGLY
                     TISLSAGAWVDVVQDGHFLKPRAFSGATDCEGIRKTMKYELAASPFVLQISGTPEQAI
                     SIAILPSD"
     gene            2885..3670
                     /gene="modA"
                     /locus_tag="VM910_33700"
     CDS             2885..3670
                     /gene="modA"
                     /locus_tag="VM910_33700"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI67499.1"
                     /translation="MQRLAGVLLSLALLCAAALSPAVAEEKTLTVFAAASMKNALDDI
                     DAAFTAKTGVKITASYAASSTLAKQIEQGAPADIFVSADTDWMDYAAARKSINEATRI
                     NLLGNSLVLIAPKDSKIGDVTIGAGFDLAKLAGDGRIATGDVKAVPVGKYAKAALEKL
                     GAWQAAEPKFAMADSVRAALTLVARGEAVLGIVYATDAKVEPGVKVVGTFPADSHPPI
                     IYPVAATAMAKPEANQYLNYLHSSAAKAVLEKYGFVYLIKPTS"
     gene            3768..4463
                     /gene="modB"
                     /locus_tag="VM910_33705"
     CDS             3768..4463
                     /gene="modB"
                     /locus_tag="VM910_33705"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HVI67500.1"
                     /translation="MFDITTTEWTAILLSLRVAIVATLVATPIGIALAWLLARRNFWG
                     KSFVDALVHLPLVLPPVVTGYLLLLTFGRRGPLGAWLADHLGIVFAFRWTGAALACGV
                     MAFPLLVRPIRLSIEAVDRRLEQAAETLGAPPWRVFLNVTLPLSLPGVLAGMVLGFAK
                     AIGEFGATITFVSNIPGETQTISSAIYSLIQTPDGDAAALRLVVVSTAIAFAALIASE
                     WFARRATQRLHGN"
     gene            4466..>4932
                     /locus_tag="VM910_33710"
     CDS             4466..>4932
                     /locus_tag="VM910_33710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI67501.1"
                     /translation="MLRVDVQKQLGEFSLSAAFASEGRVTGLFGASGAGKTSLVNMIA
                     GLMRPDRGTISIDDEILDDTEKAIHVPAHRRRIGYVFQDARLFPHLDVAQNLDYGRRM
                     NGLPDDGAHKTRVMALLDIGHLLNRRPGKLSGGERQRVALGRALLSKPRLLLL"
BASE COUNT          894 a         1633 c         1543 g          862 t
ORIGIN      
        1 gcggcaaatc ccttcgagcg gcgtttccgc gacatgcaca cgatcgcgca gcagatccag
       61 gcccgcgaca cccattacga agacgccggg agggcgatac tctcggccag gctgcagaag
      121 ccgccgacgg cgcggtagcg ccacccgaga caatcggtcc gagaggctaa agctgcgtct
      181 gcgctgacag gcaccaggca gccggatatt ctgcccgcct gttggcttgg gtgcgatcgg
      241 cgggctcggt accgcagtca tgctcccccg cgcggctcgt gcgcaggcca cgatgccggt
      301 cgtcggatgt ctcagcagct caaatgcagg gtttgcaacc tttctggccg cgttccggga
      361 gggaccgagc gaacaaggtt tcgtcgaagc aaagaacgtg aggatcgaat atcgatcggt
      421 cgacgaaagt cggtatgacc gcttgccggc gctggccaaa gaattggtcg agcatcatgt
      481 gtccgtcatc ttcgccaatc caattcctgc ggcgcccgcg gccaaagccg cggccggcgc
      541 aacccccgtg gtttttgccg tcggcagcga tcccgttggc accgggctgg tctcaagcat
      601 caaccggccc ggcggcaaca ttatcggcgt cagcttcctg acggtagcgc tcgccgccac
      661 aagtattttt cggcgagcct gattcacgcc acgatcccga cgcgatttcc ggcttcggcg
      721 gcattcgcct cacattactc gccttcagga gctaagcgac ctccgtattg ggtgcgcttg
      781 acacgcacca acaggactgc cgcatgcgca aatcaaccca ttgcgagggc cgcgcgctcc
      841 ctctttcctg atcctgcgtt cgccgcttag agtgagatca tgaacgcccg actgattctc
      901 atcccgctcc tgatgatgat gcttgcccgc gcctgcccgg cgctcgcgca ggacaagggc
      961 accgtcgacg ccaagccgct gccgccgctt gccaatccca acgaccccaa gatcggcgcc
     1021 aagcaactct tcgcgcgcaa gctgttgccg tcgtcaggac cgcccaaggt gatcggcgcc
     1081 tacaacaaag gctgcgtggc gggcgcggcg aagatgcccc tcaacggcga caactggcag
     1141 gtgatgcggc tgtcgcgcaa ccgctattgg ggatatcccg gcacgatcgc attgttgaag
     1201 cggctcgccg ccaatgtcca caaggacacg ggctggccgg gcatcctggt cggcgacatc
     1261 ggccagcccc gcggcggacc cgcgctgtcc ggccatgcca gccaccagat cggactcgat
     1321 gccgacatct ggctgacccc gatgccggat catcgcctgt cgcgcgaaga gcgcgaggaa
     1381 atgtcggcga ccatgatggt gcgcgaggac cgtctcgacg tcgatccgaa ggcatggacg
     1441 ccgcagcact ggatgttgct gcgcgacgcc gcgcaggagc ccggcgtgca gcggatcttc
     1501 gtcaatgccg caatcaagaa ggcgctgtgt cgcgaggcca agggcgaccg gagctggctc
     1561 gcaaagatca ggccatggta tggccacgac taccacttcc acatccgcat gaggtgccca
     1621 gccggcagca gcgaatgcaa gggccagtcg gatcagggcg gcggggaagg ctgcaatgcc
     1681 tccgacctcg cctactggtt caaggattcg atcatccacc ccaaaccgga gccggaaccg
     1741 aaggagccgc cgaaaccgaa ggtggggatc acgctggcgc aactgccgaa ggcttgccgc
     1801 gcggtgctga acgcgccgga cgccaagcga taagctggac tatccctgca cggccgagtt
     1861 gcgctaggat cattccgcgg gacgccgtaa gcgccccgcg tcctgcggac gatggtccca
     1921 atctcctcac ttcattcaaa cgtcgcacgc gcgtggcaaa accgcgcgcg gctcaaccgt
     1981 gcgagacaga cagatgcgca tcagtgcacg caaccagatc aggggcaccg tgatcgaagt
     2041 gaaaaagggc gcgaccactt cgcatgtccg cgtcgacatc ggcggcaatg tcgtgacctc
     2101 ctccatcacc aacgaggccg tcgatgagct cggcatcaag gcgaaggatt ccgtgatcgt
     2161 ggtgatcaag gcatccgacg tgatgatcgg ggtggactag ccatgcgtcc accactgttg
     2221 atcgcgcttg cgctgctcgg cgccgctccc gcgttcgcgg ccgaggagcc gagcggctgc
     2281 gacaagttca aatggaatat cgaccatgag cgcgccgcgc tcacgtcaac cgatcagcag
     2341 atgctcgcct cggggaccga actcaccgcg ctgcctgcaa ggggcgtgat cctggccctg
     2401 gtcgcgccgg ccgaggcgaa gctgccgtcg ccgcccgaac gtggcccgaa ggacggcacg
     2461 ctcgccggct tcgcgagctt caagacggcc cccaagacgg gcctctacac catcagcctg
     2521 tctgccggcg cctgggtcga cgtggttcag gacggccact tcctgaagcc cagagctttc
     2581 agcggcgcga ccgattgcga gggcatccgc aagaccatga agtatgaatt ggctgcgagc
     2641 cccttcgtcc tgcaaatctc cggcaccccg gaacaggcga tctcgatcgc gatcctgccg
     2701 tcggactagg gcgggccctt tacccgacgg cccaagctgt tatctttggt gcgcgatatc
     2761 cttgttcggc cgtaagcccg atgggacacg cggaacggcg cttccgcatt cgtcgcactt
     2821 caataccagc aacgcctgat ctgcgcgcgt tcgacgcccg cgcgaccggg catggagcgc
     2881 catgatgcaa cgtcttgccg gagttctgct gagcctcgcg ctcctgtgcg cggcagcgct
     2941 ttcgcctgcc gtggccgagg aaaagaccct gaccgtattc gccgcagctt cgatgaagaa
     3001 cgcgcttgac gacatcgatg cggccttcac ggcaaagacc ggcgtcaaga tcaccgccag
     3061 ttatgccgcg agttcgacgc tggcaaaaca gatcgagcaa ggcgcgccgg cggacatctt
     3121 cgtgtccgcc gacaccgact ggatggacta tgcggcggcc aggaaatcca tcaacgaagc
     3181 gacgcgcatc aatctgctcg gtaacagcct cgtgctgatt gcgccgaagg actcgaagat
     3241 cggcgacgtg acgatcggcg cgggcttcga tctggcaaaa ctggccggtg acggccggat
     3301 cgcaaccggc gacgtcaagg ccgtcccggt cggcaaatac gccaaggccg cactggaaaa
     3361 gctcggggcg tggcaggccg cagagcccaa attcgcgatg gccgacagcg tgcgcgccgc
     3421 gctgacgctg gtcgcgcgcg gcgaggctgt gctcggcatc gtctatgcga ccgatgccaa
     3481 ggtcgagccc ggcgtcaagg tcgtcggcac ttttccggcc gactctcacc cgccgatcat
     3541 ctatccggtg gcggcgaccg cgatggcaaa gccggaggcc aaccaatatc tcaattatct
     3601 gcattcgtcg gccgcgaagg ccgtgctgga aaaatacggc ttcgtctatc tgatcaagcc
     3661 gacatcctag atgagctgtg ccttggtggc atcgaactgt ccgcgggctc acttaccctc
     3721 cccaggaggg gaaggtgagc agttttacca agcgccagtc ttctgaagtg ttcgacatca
     3781 caaccacgga atggactgcg atcctgctct cgctcagggt tgccatcgtt gcgacgcttg
     3841 tcgcgacgcc gatcggaatc gcgcttgcgt ggctgctggc gcggcgaaat ttctggggca
     3901 agtcgttcgt cgacgcgctg gtgcatctgc cgctggtgct gccgccggtc gtgaccggct
     3961 atctcctgct tctcaccttc ggccggcgcg gcccgctcgg cgcgtggctg gccgatcatc
     4021 tcggcatcgt cttcgcgttc cgctggaccg gcgcggcgct cgcctgcggc gtgatggcgt
     4081 ttccgctcct ggtccgcccg atccgtctct cgatcgaggc cgtcgaccgc cgcctcgaac
     4141 aggccgcgga gaccttgggc gcgccgccgt ggcgggtgtt cctcaacgtc acgctgccgt
     4201 tgtcgctgcc gggcgtactt gccggcatgg tgctcggctt tgccaaggcg atcggcgagt
     4261 tcggcgccac tatcaccttc gtctccaaca tacccggcga gacgcagacc atttcatccg
     4321 cgatctattc gctgatccag acgcccgacg gcgatgccgc ggcattgcgg ctggtcgtgg
     4381 tatcgacggc gatcgcgttt gcggctttga tcgcctcgga atggttcgcg cgacgcgcga
     4441 cccaacgtct gcacgggaat tgacgatgct gcgggtcgac gtacaaaagc agctcggcga
     4501 gttttcgctg tcggccgcct tcgcaagcga aggccgggtc accgggctgt tcggtgcatc
     4561 cggtgccggc aagacctcac tggtcaacat gatcgcaggg ctgatgcggc ccgaccgcgg
     4621 caccatctct atcgacgacg agatcctcga cgacaccgaa aaggccattc atgtcccggc
     4681 ccatcgtcgc cgcatcggtt atgtgtttca ggatgcacgg ctgtttccgc atctcgatgt
     4741 cgcgcagaac ctcgactatg ggcggcgcat gaacggcctt cccgacgatg gcgcgcacaa
     4801 gacgcgcgtc atggcgctgc tcgatatcgg ccatctgctc aatcgccggc caggcaaatt
     4861 gtcgggcgga gagcgccagc gtgtcgcact cggacgcgcg ctgttgtcga agccgcggct
     4921 gttgctgctc ga
//
LOCUS       DATLJT010001469         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4039304,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001469 DATLJT010000000
VERSION     DATLJT010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039304"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VM910_33715"
     CDS             <1..244
                     /locus_tag="VM910_33715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HVI67502.1"
                     /translation="VLVLDTAKGGVATAWMLHEMKSRGIVPLALVLNSVNPILAQGAA
                     LGDVAMLAGFDEDITAAVSHGAEVEVVPQQSLLRVI"
     gene            253..651
                     /locus_tag="VM910_33720"
     CDS             253..651
                     /locus_tag="VM910_33720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVI67503.1"
                     /translation="MDARDVITIGMSAERRLVVPPEQTVGHLLAGMPMVFATPMMILQ
                     MELASGDAIRTHLHPGWVTVGTEVDIKHLAASPVGATVRTTSKVIAVERRVIRFEVAS
                     FDGERKIGEGRHARGLVNIQAFNKRLGGAG"
     gene            complement(684..1514)
                     /gene="proC"
                     /locus_tag="VM910_33725"
     CDS             complement(684..1514)
                     /gene="proC"
                     /locus_tag="VM910_33725"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVI67504.1"
                     /translation="MTPLNNSNALQNIAGTIALAGAGKMGGAMLTGWLAQGLDAQRVV
                     VFEPAPSAEIAALAARGVRLNPKDAGAISTLVIAVKPQSFREAGAALKSHVAPATLVV
                     SIMAGTTIKSLQETVGGAVVRAMPNTPAAIGRGITVAVADKGVSAAQRATADALLRAT
                     GAVEWVDDEKLMDAVTAVSGSGPAYIFLLAEELARAGVAAGLPEELAARLARETVAGS
                     GELLHRSDLAAAALRQNVTSPGGTTAAALEVLMGKDGLQQLMIRAIAAATARSKELAK
                     "
     gene            complement(1679..2179)
                     /locus_tag="VM910_33730"
     CDS             complement(1679..2179)
                     /locus_tag="VM910_33730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HVI67505.1"
                     /translation="MSMLEGIVESRSHPIAVVEDVASDNNWSFERSGEDEITIVTRGE
                     WTDYQLSFTWMGEIEALHLACAFDMKVPPVRRAEVQRLVAAVNEQLWIGHFDLWTHTG
                     LIMHRQALVLPGGITASSAQCENMLVGAIHACERYFPAFQFVVWAGKSAAEAMNAAMF
                     DTAGEA"
     regulatory      2595..2755
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2832..>3134
                     /locus_tag="VM910_33735"
     CDS             2832..>3134
                     /locus_tag="VM910_33735"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVI67506.1"
                     /translation="MNQMSQEPVQESQSQDAEAQSAASPATPEPRPRPAHPRFAKPQR
                     VAFVQSAWHREVVAECRMAFLDEAEARNIPRAAIDVFEVPGSFEIPLHVQILAKTRR"
BASE COUNT          550 a          986 c         1015 g          583 t
ORIGIN      
        1 ggtcctggtg ctggacacgg caaaaggcgg cgtcgcgacg gcatggatgc tgcatgagat
       61 gaaatcgcgc ggcatcgtgc cgctggcgct cgtgctgaac tcggtcaacc cgatcctcgc
      121 gcaaggtgcg gcgctcggcg acgtcgcgat gctcgcagga ttcgacgagg acatcaccgc
      181 agcggtttct cacggcgccg aggtggaagt tgttccgcaa caaagcttgc tcagagtgat
      241 ataggttttg gcatggacgc acgcgacgtc ataacaatcg gcatgagtgc ggagcgcagg
      301 cttgtggtgc cgccggagca gacggtcggc catctgctcg cgggcatgcc gatggtgttt
      361 gcgacaccga tgatgattct gcagatggag cttgcctccg gcgatgccat ccggacgcat
      421 ctccatccgg gctgggtcac cgtcggcact gaggtcgata tcaagcatct cgcggcttcg
      481 ccggtcggcg cgacggtgcg cacgacctcg aaggtgatcg cggtggagcg ccgcgtgatc
      541 cgtttcgagg tcgcttcgtt cgatggcgaa cgcaagatcg gcgaggggcg tcacgcccgc
      601 ggactggtca atatccaagc tttcaacaag cggctgggcg gggcggggta agtactccgc
      661 tcgtcatgcg ctgttgacgg gttttacttc gccaattcct tcgagcgtgc ggtggccgcc
      721 gcgatcgcgc ggatcatgag ttgctgcaag ccatccttgc ccattagcac ctccagtgct
      781 gcggccgtgg tcccgcccgg cgaggtcacg ttctggcgca gagccgccgc ggcaagatcg
      841 gagcgatgca agagctcgcc cgagccggcg acggtttcgc gcgcgagcct tgccgccagc
      901 tcctcgggca agccggctgc gacgccggcg cgcgccagtt cttccgccag caggaagatg
      961 taagcgggac cggagccgga gacggcggtt accgcatcca tcagtttttc gtcatcgacc
     1021 cactcgaccg ctccggtcgc gcgcagcagc gcgtcggcgg tcgcgcgctg cgccgcgctg
     1081 acacctttgt cggcgaccgc aacggtgatg ccgcgcccga tggcggccgg cgtgttgggc
     1141 atcgcccgca ccacggcgcc gccgacggtt tcctgcaacg acttgatcgt cgtgcccgcc
     1201 atgatcgaga ccacgagcgt tgcgggcgcg acgtgcgatt tcagtgcggc gcccgcctcg
     1261 cggaacgact gcggcttgac cgcgatcacc agcgttgaga tggcgccggc atccttcggg
     1321 ttgagccgga cgccgcgcgc ggcaagcgct gcgatttccg ctgacggcgc aggctcgaat
     1381 accacgacgc gttgtgcatc gaggccctgc gccagccagc cggtcagcat cgcgccgccc
     1441 atcttgcccg cgccggcgag tgcgatggtg ccggcgatgt tttggagagc gttgctgttg
     1501 ttcaaaggcg tcaccacaaa tacagccctc gtcccggcgg aagccgggat ccataaccac
     1561 aggagcggtt ctatttgatc gcgatatcgg cgacaagcac cgtgcgatgc aactggcgtc
     1621 gcgcggtatt gatcccggat cgcgctcgct ttcgctcgct tgtcggggat gacggcggtt
     1681 aggcttcgcc cgcggtgtcg aacatcgcag cgttcatggc ttcggccgcc gacttgcccg
     1741 cccacaccac gaactggaac gccggaaaat agcgctcgca ggcgtggatc gcgcctacca
     1801 gcatgttctc gcattgcgcc gaggaggctg tgataccgcc cggcagcacc aaggcctggc
     1861 ggtgcatgat caagccggtg tgggtccaga ggtcgaaatg gccgatccac aattgctcgt
     1921 tgacggcggc gaccagccgc tgcacctcgg cgcggcgcac gggcggcacc ttcatatcga
     1981 aggcgcaggc cagatgcagc gcctcgatct cgcccatcca ggtgaaggag agttgatagt
     2041 cggtccactc tcccctggtg acgattgtga tctcgtcttc gccggagcgc tcgaacgacc
     2101 agttgttatc agatgcgaca tcctcgacca ccgcaatcgg gtggctgcgg gattcgacaa
     2161 tgccttcgag catggacatg ccgtctcaac ccttattttc tttgttcgtt gatacgaact
     2221 caaaacccgg cacgcgcagc aatgtgggca gcgtggctac cggatgcgac cccgaggagg
     2281 tcccgggctt gccgggatca actgatgtga tttgcggaat ctgcgcaacc acggccagag
     2341 tccgtccaca tcattgcagc gcgctgtcca caacagatcg tgctccaaat ttgttaacgg
     2401 gagaacaaac cggaatcgga tcggctctta agacatcgtt aactttttcg tcgatgcgat
     2461 tcgaggcgaa gttgtgggaa atcttcagag ctttctcgcg acccgcatgg tgcgtatgtc
     2521 ggttccgagg gcaaagcagc ttgcccgcct gtcggccttt ggccatagtt gttaacggct
     2581 atccatgtcg gatggcctgt tctcagggcg gggtgaaatt ccccaccggc ggtaagggcg
     2641 acgaatcgga aagcggcccg agcccgcgag cgccttcttt cgcgaggagg aggggtcagc
     2701 agattcggtg caattccgaa gccgacggtg acagtccgga tgaaagagaa cggtccgcgc
     2761 ggccagcgtc tttgcgcgca ggcttcgctg tcccgtgtgc cctggttctg gtcctgacga
     2821 agaggcaaac catgaatcag atgtcgcaag aaccagttca ggagtcccag tctcaagacg
     2881 ccgaggctca atccgccgcc tcgccggcaa cgccggagcc gcggccaagg cccgcgcatc
     2941 cgcgatttgc caagccgcag cgggtcgcct tcgtacaatc cgcctggcac cgcgaggtcg
     3001 ttgcggagtg ccgcatggcc tttctcgacg aggccgaagc gcgcaacatc ccgcgcgctg
     3061 caatcgacgt cttcgaggtg ccgggctcat tcgagattcc gctccacgtg cagattctcg
     3121 ccaagacgcg ccgc
//
LOCUS       DATLJT010001470         8546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_819002,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001470 DATLJT010000000
VERSION     DATLJT010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819002"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..553
                     /locus_tag="VM910_33740"
     CDS             158..553
                     /locus_tag="VM910_33740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVI67507.1"
                     /translation="MTKEPINWMLSEAIGSLARAERLRQQFFNLQSTGGPCWEPPIDV
                     LETDGELLIFVALPGVDPDQVEALIDNGVLVISGNRTLPAELRNARIHRLELPQGRFE
                     RRIGLPPGRYAISRHSVNGCIVFRLAKSG"
     gene            558..2954
                     /gene="lon"
                     /locus_tag="VM910_33745"
     CDS             558..2954
                     /gene="lon"
                     /locus_tag="VM910_33745"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HVI67508.1"
                     /translation="MTTDNEQSNAAASATGQKPVQLPDDSLIIVAVRNIVLFPGVVAP
                     ITINRPKSIAAAQQALREQRPIGILLQRNAEVEDPTPDDLYRVCTVANLVRYITAPDE
                     THHIVCQGVQRARVLDFFPGTPFLVARILRIPEPTTSSPEIEARFLNLQRQAIEAIQL
                     LPQTPPELLAAFQSTTSPAALADLATSYMDIKPQEKQEILETVDLSKRMEKVSQYLAE
                     RLEVLRLTNEIGQKTKAAFDERQREAILREQMATIQRQLGEGDGKAQEVAELTEAIAK
                     AQMPSEAESQARKELRRYERMPDAAAEAGMVRSYLDWLIELPWALPPEKPIDIAAARR
                     ILDEDHFGLEKIKSRIIEYLAVRKLAPQGKAPILCFVGPPGVGKTSLGQSIARAMDRP
                     FVRVSLGGVHDEAEIRGHRRTYIGALPGNIIQAIKKAGARNCVMMLDEIDKMGRGIQG
                     DPSAAMLEVLDPEQNATFRDNYLGVPFDLSRVVFIATANMLDTIPGPLQDRMEIISLP
                     GYTEEEKLEIARRYLVRRQLEANGLSPEQAEIDPEALRLTIKGYTREAGVRSLEREIG
                     RALRHAAVQIAEGSTAKVVIAPKDLSAILGPPRFEGEIALRTSVPGVATGLAWTPVGG
                     DILFIEATRVPGKGGLILTGQLGDVMRESVQAALTLVKSRASQLGIDPAVFERSDIHV
                     HVPAGATPKDGPSAGVAMFTALASLLTNRTVRHDTAMTGEISLRGLVLPVGGIKEKVV
                     AAAAAGLSRVMLPARNRRDFEEIPKSAREKLTFIWLEQVDDAIANALAEAKPPAAAAE
                     "
     gene            3043..3435
                     /locus_tag="VM910_33750"
     CDS             3043..3435
                     /locus_tag="VM910_33750"
                     /inference="COORDINATES: protein motif:HMM:NF014968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HVI67509.1"
                     /translation="MARTAALATVLVTTLLAGCWSTDRPVAESASMGSVAAAPPINLA
                     GRWTLSSTGSGSCTMTFGAQPEASEGTIAPAGGCPFNFFTSRKWSYTTTGLTIRDHNA
                     LALVQLVPAGSDRFEGKTSAGQDVVLSR"
     gene            complement(3424..4248)
                     /locus_tag="VM910_33755"
     CDS             complement(3424..4248)
                     /locus_tag="VM910_33755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI67510.1"
                     /translation="MNMAGKTVLITGSTDGVGRYVAGALAKLGAELLIHGRDAPRAKS
                     LIDDIAASGHAPPTFYQADLSSLDSVRKLASTIASRHKRIDVFISNAGIGSMNGGPLR
                     QVSADGYELRFAVNYLSGFLLAYLLLPLIKAAAPARIVNVASLGQHPIDFDDVMIVKN
                     YSGSRAYAQSKLAQIMFTIDLANELEGTGVTVNSLHPATYMNTTMVRAGGITPISTVE
                     EGGAAILHLATGDDVAGKSGLFFNGMNETRANPQAYDAEARRRLRRLSLELTGLSG"
     gene            complement(4266..4985)
                     /locus_tag="VM910_33760"
     CDS             complement(4266..4985)
                     /locus_tag="VM910_33760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HVI67511.1"
                     /translation="MTTSAAAPLPASVLEALKRYDTPTICNAMEVVAPERRLIGYTTK
                     PLVCPFPDLPPIVGYARTVAIRSVIKSGLSVAEQAARRIAYYEYVGTGFGPRISVIQD
                     IDGADVGYGAFWGEVQSNVHKALGCLGVVTDGSIRDIPQWAVGFQALAGSIGPSHAWV
                     HAENFGGEVRVAGMTVKSDDLIHADRHGAIVIPYDLAAKLPEACELCGRRETPILEIA
                     RAKDFSLEKLKEALKRSAEIH"
     gene            complement(5109..5990)
                     /locus_tag="VM910_33765"
     CDS             complement(5109..5990)
                     /locus_tag="VM910_33765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HVI67512.1"
                     /translation="MPFDPRYADLRRRRFRPIPVRMLVPNVITLLAICAGLTAIRLST
                     EGRMELAVAAIVFAAVLDGIDGRIARMIKGQSKFGAELDSLADFVNFGVAPGLMLYFW
                     QLHELHNGGWIAAMVFAISGGLRLARFNASIDDPNKPSFAANYFTGVPAPAGAVTALL
                     PIYLANLGVFKSPATLTAAYTLLIAFLMVSRLPVFSGKSVRMRVPPELVLPLFVCVIF
                     FIALLVGYPWYILSIGTVLYLLSLPLGWKSYRDQARAAGLTAVPAANDVQPPVSAPAF
                     TSAVTDSSEDDRPERLH"
     gene            complement(6089..6790)
                     /locus_tag="VM910_33770"
     CDS             complement(6089..6790)
                     /locus_tag="VM910_33770"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HVI67513.1"
                     /translation="MSIANSIRAQIPPIHPEGYPFIGGFALASLILFWIWGPLGWIGT
                     LLTVWCALFFRDPVRVTPVRDGIVVAPADGRISMITQAIPPPELGLGDKALPRISIFM
                     SVFNCHINRSPVAGRIDRIAYRPGAFINAELDKASEDNERNSLVIDTPNGRIGVIQIA
                     GLVARRIVSFVKEGQSIGAGERFGLIRFGSRLDVYLPEGTKALVCEGQTAIAGETILA
                     DFRLNDGLTPAFRSA"
     gene            complement(6936..>8546)
                     /locus_tag="VM910_33775"
     CDS             complement(6936..>8546)
                     /locus_tag="VM910_33775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HVI67514.1"
                     /translation="QWRDGIFARVAMHAVRKLAYITFVHMHELSLRFHLERKTGGLTR
                     ILERGRNGIETIVRMVILQLIPTIVEVSLLMVVLFWQFDWRYVLATLITVVVFMYFTY
                     AATEWRIAIRRKMNDSDTEANTKAIDSLLNYETVKYFGAETREAQRYDRSMERYEQAS
                     VKTYTSLAVLNTGQAVIFTFGLTATMLMCAIGVRQGRNTVGDFVLINAMMIQLYQPLN
                     FMGMVYREIKQAIIDIEKMFNVLEQRPDIKDVPGAKPLVVTAGSLRFDDVRFAYEPER
                     PILKGLSFEVPAGKTVAIVGPSGAGKSTISRLLFRLYDVSSGTISIDGQDVRSVTQAS
                     LRAAIGMVPQDTVLFNDTIRYNIRYGRWDATDAEVERAAQLAQIDGFIRMSPKGYETQ
                     VGERGLKLSGGEKQRVAIARTVLKTPPILVLDEATSALDSHTEHEIQEALDRVSRNRT
                     SLVIAHRLSTIVSADEIIVLDQGLIVERGTHGELLAKGGLYASMWNRQREAEAARERL
                     ALVDDTNDAPNRELPPPVDDTPVAAAAE"
BASE COUNT         1680 a         2714 c         2640 g         1512 t
ORIGIN      
        1 aagacgcgta aaggttaagc agcgaccttt cgcaaccggt aaggcgcacc gttggaggca
       61 ggcgaaaaca cgccatggtg agcacggccc ccggccttcc tgacaccgcc tcaaccttct
      121 gctaaccata acgaacggga gagccaccga ggaacaaatg acaaaagaac ccatcaactg
      181 gatgctgtcg gaggccatcg gctcgcttgc ccgtgcggag cggctgcgtc agcagttttt
      241 caacctgcaa tcgacgggcg ggccgtgctg ggaaccgcct atcgacgtcc tggaaaccga
      301 cggggaactg ctcatcttcg tcgcgctgcc gggggttgat cccgatcagg tcgaggcact
      361 gatcgataat ggcgtgctcg tgatcagcgg caacaggacg ctccccgcgg agctgcgcaa
      421 cgcccgtatc catcggctcg aactcccgca aggccggttc gagcgccgca tagggctgcc
      481 tccgggacgc tacgcgatca gccgccattc cgtgaacggt tgcatcgtct tccgcttggc
      541 gaaatcaggt tagtcccatg acgactgaca acgaacaatc aaatgctgct gccagcgcaa
      601 ccggtcaaaa acccgttcag cttccggacg atagtttgat cattgttgca gtccgcaaca
      661 tcgtgctgtt tcccggtgtg gtcgcaccga ttaccatcaa ccggccgaaa tcgattgcgg
      721 ccgcccagca ggcgctgcgc gaacaacggc cgatcggaat ccttttgcag cgaaacgccg
      781 aagtggaaga tcccacgccg gacgacctct atcgtgtctg cacggtcgcc aatctcgtgc
      841 gctacatcac cgcaccggat gaaacccatc acatcgtctg ccagggcgtc cagcgcgccc
      901 gcgttctcga cttttttccc ggtacgccgt tcctcgttgc gcgcatcctg cggattcccg
      961 aaccgacgac aagctcaccc gagatcgaag cgaggtttct caacctgcaa cggcaggcaa
     1021 tcgaggcgat acagcttctg ccgcagacgc cacctgagct tctggccgcg ttccagtcga
     1081 ccacgtctcc cgcagcgctc gccgacctcg ccacctccta catggacatc aagcctcagg
     1141 agaagcagga aatcctggag accgtcgacc tttccaagcg aatggagaaa gtttcacagt
     1201 accttgcgga gcgactggaa gtcctgagac tgacgaatga gatcgggcag aagaccaaag
     1261 cggcattcga cgagcgccaa cgtgaggcca tcctgcggga gcagatggcg accatccagc
     1321 gccagctcgg cgaaggcgac ggcaaggccc aggaggtcgc ggagctgacc gaagccatcg
     1381 ccaaggcgca gatgccttcc gaagctgaaa gccaggcacg caaggagcta cgccgttacg
     1441 aacggatgcc cgatgccgcg gccgaagccg gtatggtccg cagctatctc gactggctga
     1501 tcgaactgcc ctgggcactg ccgccggaaa agccgatcga cattgccgcg gctcgccgca
     1561 ttctcgacga ggaccatttc gggctggaaa aaatcaaaag ccggatcatc gaatatctgg
     1621 ccgtgcgaaa gctggcgccg cagggcaagg cgccgatcct ctgcttcgtc ggcccgcccg
     1681 gcgtcggcaa gacgtcactc ggacaatcga ttgcacgcgc catggaccgg ccgttcgtca
     1741 gagtgagtct tggcggcgtc catgacgaag cggaaatccg cggccatcgt cgcacctata
     1801 tcggtgcgct acccggcaac atcatccagg cgatcaagaa ggccggcgcg cgcaattgcg
     1861 tgatgatgct cgacgagatc gacaagatgg ggcgcggcat tcagggcgat ccgtcggcgg
     1921 cgatgctcga ggttctcgat cccgagcaga atgcgacctt ccgtgacaat tatctgggcg
     1981 tgccgttcga cctgtcgcgt gtcgttttca tcgcgaccgc aaacatgctc gataccattc
     2041 cgggccctct gcaggatcgc atggagatca tcagcctgcc cggctacacc gaggaagaaa
     2101 agctcgagat cgcgcgccgc tatctggtac gacgccaact cgaggccaac gggctttccc
     2161 ccgagcaagc cgagatcgat cccgaggcgt tgcggctcac tatcaagggt tacacgcgcg
     2221 aggccggcgt gcgctcgctc gagcgcgaaa tcgggcgggc gctgcggcat gcggcggtgc
     2281 agatcgccga gggcagcacc gcgaaggtgg tcatcgcgcc gaaggacctc agtgcgatcc
     2341 tcgggccgcc gcgctttgag ggtgagatcg ccttgcgcac cagtgttcca ggcgtagcaa
     2401 ccgggctcgc gtggacgcct gtcggcggcg acatactgtt catcgaggcg acacgcgttc
     2461 ccggcaaggg cggactgatt ttgactggcc aactcggcga cgtcatgcgc gagagcgtac
     2521 aagccgctct cacgctggtg aagagccgcg cctcccagct cggcatcgat cctgcggtct
     2581 tcgaaaggag cgacattcac gttcacgtgc cggcgggcgc gacgccgaaa gatggtccga
     2641 gcgcgggcgt tgccatgttc accgcgctgg cttcgctgct gaccaaccgc accgtgcggc
     2701 atgatacggc catgaccggg gaaatctcgc tgcgcggcct ggtgttgccg gtcggtggca
     2761 tcaaggagaa ggtcgttgct gccgccgccg ctggcctgag ccgcgtcatg ttgcctgcac
     2821 gcaatcgccg ggatttcgag gagatcccga aaagcgcgcg ggagaaacta acattcatct
     2881 ggctcgaaca ggtcgacgat gcgatcgcca acgcccttgc ggaggccaag ccgccggctg
     2941 cagccgccga gtgatcgtgg cgggcagttt cgcgctgatc ctgacttgaa cgcccctttc
     3001 tgctaattca tcctcgcagc tcgctgcttc atctgctgat acatggcccg taccgctgcg
     3061 ctcgcgaccg ttctcgtcac caccctgctc gctggctgct ggagcaccga tcgtcccgtg
     3121 gcggagagcg cgagcatggg ctcggtcgca gccgcgcctc cgatcaatct tgcaggccgc
     3181 tggacgctgt catcgacggg ttcgggatca tgcacaatga ccttcggcgc gcagcccgag
     3241 gccagcgagg gcacgattgc gccggcaggc ggctgccctt tcaatttctt caccagccgg
     3301 aaatggagct acaccacgac cggcctcacc attcgcgacc acaatgcgct ggcgctggtt
     3361 caactcgttc cggcaggatc cgaccgtttc gagggcaaaa ccagcgcggg ccaggacgtg
     3421 gtgctatcca gatagcccgg tcagttcgag gctaagcctg cgcagccgtc ggcgcgcctc
     3481 agcgtcataa gcctgcgggt tggcgcgcgt ctcattcatg ccgttgaaga acagcccgct
     3541 cttgcccgcg acatcgtcgc cggtcgccag gtgcaggatc gccgccccac cctcctcgac
     3601 cgtcgagatc ggcgtgatgc cgccggcgcg caccatggtg gtattcatgt aagtcgccgg
     3661 atgcagcgaa ttgacggtca cgccggttcc ctccagctcg ttcgccaggt cgatcgtgaa
     3721 cataatctgc gcgagcttgc tctgcgcata ggcgcgcgag cccgagtagt ttttcacgat
     3781 catcacgtcg tcgaaatcga tcgggtgttg accgagcgaa gcgacattga cgatgcgcgc
     3841 gggcgcggcc gccttgatca gcggcagcag gagataggcc agcaggaaac ccgaaagata
     3901 gttcaccgca aagcgcagct cgtagccgtc ggcgctgact tgtcgcaggg gcccgccgtt
     3961 catcgatccg atgccggcat tgctgatgaa gacgtcgatc cgcttgtgcc gggatgcaat
     4021 agtgctcgcg agcttccgca cgctgtcgag cgacgacagg tcggcctgat agaaggttgg
     4081 cggggcatgg ccgcttgcag ctatgtcgtc aatcaacgac tttgcgcgtg gcgcatcacg
     4141 gccgtgaatc aacagctccg cgcccagctt tgcgagtgcg cctgcgacgt agcggccgac
     4201 gccgtcggtc gagcccgtga ttaggaccgt cttacctgcc atgttcatgg ctttgaacca
     4261 tcgcactaat ggatttcagc cgagcgcttg agtgcctcct tcagcttctc cagcgaaaaa
     4321 tccttggctc gcgcaatctc gagaatcggc gtctcgcgcc ggccgcagag ttcgcaagcc
     4381 tcgggcagct tggctgcgag gtcgtagggg atcacgattg caccgtgccg gtcggcatgg
     4441 atcagatcgt cagacttcac agtcatgccg gcaacccgaa cctcgccgcc aaaattttca
     4501 gcgtgcaccc aggcatgcga cggcccgatg gagccggcga gtgcctgaaa accaaccgcc
     4561 cattgcggaa tatcacggat cgagccgtcg gtaacgactc cgaggcagcc gagcgccttg
     4621 tgcacgttgc tctgcacctc accccaaaac gcgccgtatc cgacgtccgc gccgtcgatg
     4681 tcctggataa ccgagatgcg tggcccaaaa ccggtgccga catattcgta gtaggcgatg
     4741 cgccgcgccg cctgttcggc cacggaaagg ccggacttga tcaccgagcg gatcgctacc
     4801 gtgcgagcat agccgactat cggcggcaga tcgggaaacg ggcagaccaa aggttttgtc
     4861 gtgtagccaa tcagccgtcg ctccggcgcc acgacctcca tggcgttgca gatggtcggg
     4921 gtatcgtatc gcttgagcgc ttcgaggacg gatgcgggaa gcggcgcagc agcggaagtg
     4981 gtcacaggcg tttctccgga ttttggtgtg gcgagacaat cgtcgcccat tgacagccct
     5041 atagcggagt tctcgcgcga acccaactca ccagcagtcg catcggaaaa agatcgcgct
     5101 cgcaggcttc agtgcagccg ttccggccgg tcatcctcag agctatcggt gaccgccgac
     5161 gtgaatgccg gcgccgacac cggcggctga acgtcattcg cggccggtac ggccgtcaag
     5221 cctgccgcgc gcgcctggtc gcgataggat ttccacccca acggcaggct caggagatag
     5281 agcaccgtgc cgatggacag gatgtaccag ggatagccga ccaacagcgc gatgaagaag
     5341 atcacgcaga cgaacagcgg caacaccagc tccggcggta cgcgcatgcg cacgctcttg
     5401 cccgaaaaca ccggcagccg cgacaccatc aggaaggcga tcaaaagcgt ataggccgcc
     5461 gtcagcgtcg ccggcgactt gaacacgccg aggttggcga gatagatcgg caacagcgcg
     5521 gtcaccgcac ccgccggcgc cggcacaccg gtaaaatagt ttgccgcaaa cgacggcttg
     5581 ttcggatcgt cgatcgatgc gttgaagcgc gccagccgca agccgccgga gatcgcaaac
     5641 accatcgcgg cgatccagcc gccattgtgc agctcgtgca gttgccagaa ataaagcatc
     5701 aggccggggg cgacgccaaa attgacgaag tcggcgaggc tatcgagctc ggcaccgaat
     5761 ttcgactgtc ccttgatcat gcgcgcgatg cggccgtcga tgccatcgag cacggccgcg
     5821 aacacgatcg cggccaccgc gagttccatt cgcccttcgg tggacagccg gattgccgtc
     5881 aaccccgcgc agatcgcaag cagcgtgatg acattgggca ccagcatacg caccggaatc
     5941 ggacgaaacc ggcggcggcg caagtcggcg tatcggggat cgaagggcat gcgctcaata
     6001 tagcaagtgc tgtcggcttg cgccattgcg gcggcgcgac gcgacagagc acattaccaa
     6061 tggttaattc gtgcggcgcg gcaacgcgtc atgcgcttcg gaaggctggc gtcaggccat
     6121 cgttcaggcg gaaatccgcc aggatggttt cgcccgcgat cgcggtctgg ccttcgcaga
     6181 ccaacgcctt ggtgccctcg ggcagataaa cgtcgaggcg cgacccaaaa cggatgagtc
     6241 cgaagcgctc gcctgctccg atcgactgtc cctccttcac gaaggacacg atgcgccgtg
     6301 ccacgaggcc ggcgatctgg atcacgccga tccggccgtt cggagtgtcg atgacaagcg
     6361 aattgcgctc attgtcttcg ctcgccttgt cgagttcggc attgatgaac gcgcccggcc
     6421 gataggcgat gcgatcgatc cggcccgcga ccggggagcg gttgatgtgg cagttgaaga
     6481 cgctcatgaa gatcgagatg cgcggcagcg ccttgtcgcc gaggccgagt tcgggtggag
     6541 ggatcgcctg cgtgatcatc gatatgcgtc cgtccgccgg cgccaccacg atgccgtcgc
     6601 gcacgggggt gacccggacc ggatcacgaa agaacagcgc gcaccagacg gtcagcaggg
     6661 tgccgatcca tccaaggggt ccccagatcc agaacaagat cagacttgcc agcgcaaagc
     6721 cgccgatgaa cgggtacccc tccgggtgga ttggcggaat ttgcgcgcgg atggaattgg
     6781 caatggacat caggttttgc tgatcgacgt taaagttcta gctcactctt cgccctgagg
     6841 gcggcgcgac gcgcaacggc tcgccgcggc aactcattat tccagaagcg tcgcgtgcga
     6901 aacccctgat ctcgcagctt cccccaaggc agcaatcact ccgcagccgc cgcaaccggc
     6961 gtgtcatcga ccggtggcgg cagctcacgg ttgggcgcat cgttcgtgtc gtcgaccagc
     7021 gccagccgct cgcgtgctgc ctcggcctcg cgctgcctgt tccacatgct ggcataaagc
     7081 ccgccttttg ccagaagctc accatgggtc ccgcgctcga cgatcagacc ctggtcgagc
     7141 acgatgatct cgtcggcgct gacgatggtg gagagccgat gcgcgatgac gagagaggtg
     7201 cgattgcgcg aaacccgatc cagcgcctcc tgaatctcgt gctcggtatg gctgtcgagc
     7261 gccgaggtcg cctcgtccaa caccaggatt ggcggtgtct tcaagacggt gcgcgcgatc
     7321 gcaactctct gcttttcccc gcccgacaat ttcaggccgc gttcgccgac ctgggtctca
     7381 tagcccttcg gcgacatgcg aatgaagccg tcgatctgcg caagctgcgc ggcccgctcg
     7441 acctcggcat cggttgcgtc ccagcggcca tagcggatgt tgtagcgtat ggtgtcgttg
     7501 aagagcacgg tatcctgcgg caccatgccg atcgcggcgc gcagcgaggc ctgcgtgacg
     7561 ctcctgacgt cctggccgtc gatcgagatc gtcccgctcg agacgtcata aaggcggaac
     7621 aggagccgcg aaatggtcga tttgccggcg cccgaaggac cgacgatcgc gaccgtcttg
     7681 ccggccggca cctcgaaact caggcctttg aggatcggcc tctcaggctc gtatgcgaag
     7741 cgcacgtcgt cgaagcgcag gctgcctgcg gtgacgacga gaggtttcgc gcccgggaca
     7801 tccttgatgt ccgggcgctg ctccagcacg ttgaacatct tctcgatatc gatgatcgcc
     7861 tgcttgatct cgcgatagac catgcccatg aaattcaacg gctggtagag ctgaatcatc
     7921 atggcgttga tcaggacgaa gtcgccgacc gtgttgcgtc cctggcgcac gccgatcgca
     7981 cacatcagca tggtcgcggt caatccaaac gtgaaaatca ccgcctgtcc ggtgttgagc
     8041 accgcgaggg aggtataggt cttcacgctt gcctgctcgt agcgctccat cgagcggtcg
     8101 tagcgttggg cctcgcgcgt ctcagcgccg aaatatttga ccgtctcata gttcagaagc
     8161 gagtcgatcg ctttcgtgtt cgcttcggtg tcggaatcgt tcatcttgcg gcggatggcg
     8221 atccgccact cggtcgcggc ataggtgaag tacatgaaca ccacgaccgt gatcagcgtt
     8281 gccagcacgt agcgccagtc gaactgccag aacagcacga ccatcagcag cgaaacctcc
     8341 acgatggtcg gaatgagctg caggatcacc atccgcacga tggtttcgat gccgttgcgg
     8401 ccacgctcca ggatgcgtgt cagtccgccg gtcttgcgtt cgaggtgaaa gcgcagcgac
     8461 aattcgtgca tatgcacgaa ggtgatatag gctagcttgc gcactgcgtg catggcgacg
     8521 cgggcgaaga tgccgtcgcg ccattg
//
LOCUS       DATLJT010001471         1800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_819009,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001471 DATLJT010000000
VERSION     DATLJT010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819009"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VM910_33780"
     CDS             <1..256
                     /locus_tag="VM910_33780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67515.1"
                     /translation="GLPTAFQRRENVEAGGLLSYGPNLMDQWRQAAVYVDRILKGALP
                     AEMPVEQPTKFELAINLKTAKALGLDVPLHLQQLANEVIE"
     gene            385..1419
                     /locus_tag="VM910_33785"
     CDS             385..1419
                     /locus_tag="VM910_33785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI67516.1"
                     /translation="MQITTIGLDLAKSVFQVHGIDAAGQVVVRKSVRRAQMLAFFSKL
                     PPCLVGIEACGTSHYWARELIRLGHEVRLMPPAYVKPYVKRGKTDAADAEAVCEAVTR
                     PTMRFVPVKSPEQQAALSMHRARDLLVKQRTQLINMLRGLLAEFGVDIPKGLERALLM
                     ARQVIEGNAPEVPIEAARIATTLSQQALDIHVRLRQIDRDLAIWLRGNDVARRLMTIP
                     GIGTVGATALVASVTDPHQFRSGRQFAAWLGLTPLQKSSGGKERLGRISKMGDKYLRK
                     LLIVGMTSLVRRAKYAPDKIDPRLADLLARKPTRVATVAMANKTARVIWAIMARGETY
                     RTGHQPALAA"
BASE COUNT          398 a          504 c          551 g          347 t
ORIGIN      
        1 ggggttgccg accgcgttcc aacgccggga gaacgtcgaa gcgggtggcc tcctatcata
       61 tgggccgaac ttgatggacc agtggcgtca agcggctgtc tatgtcgatc gtatcctgaa
      121 aggagcgttg ccagcggaaa tgccggtaga gcaacccacc aaatttgaac tggcgatcaa
      181 tctcaaaacc gcaaaagccc tcggcctcga cgtaccgctg catctccaac agctcgccaa
      241 cgaggtgata gaatagaatt tgctgcggtg catgagtccg cttatggcct aagcagatga
      301 ggtggatggc tcccgctcac ggcatctagg tgccaaggtg tgaatgccat caggcgttca
      361 cgagcaaaaa gggagccatc cgttatgcag attaccacga tcggcctgga cttggccaag
      421 agcgtttttc aggtgcacgg aattgatgcg gccggccagg ttgtggttcg caagtcggtt
      481 cggcgggcgc agatgctggc attttttagc aagttgcctc cttgcctggt cggcatcgag
      541 gcatgcggta cgtcacatta ctgggcgcgc gagctgatca ggctcggtca tgaggtgcgt
      601 ctgatgcctc ctgcttacgt aaagccgtat gtgaagcgcg ggaagaccga cgcggccgat
      661 gcggaagcgg tttgcgaggc agtgacacgg cctacgatgc ggttcgtgcc ggtcaagtcg
      721 ccggagcagc aggcggcgct atcgatgcac cgggcgcgcg atctgctggt caagcagcgc
      781 acgcagctga tcaacatgct ccgtggtcta ctggccgaat ttggcgtcga catccccaag
      841 gggctggagc gggcgctgct gatggcgcgg caggtcatcg agggcaatgc gcccgaggta
      901 ccgattgaag ctgccaggat cgccactacg ctgtcacagc aggcgctcga catccacgtt
      961 cggctccgcc agatcgatcg cgatctcgct atctggctcc gaggcaacga tgttgcacgt
     1021 cgtctcatga ctatccccgg catcgggacg gtcggcgcaa cggcgcttgt cgcatcggta
     1081 accgatccgc atcagttccg ctcgggacga cagttcgctg cctggttggg attgacgcca
     1141 cttcagaagt cgagcggtgg caaagagcgg ctgggtcgta tcagcaagat gggcgacaaa
     1201 tacctgcgca agctgctcat tgttggcatg acctcacttg tacgccgtgc aaaatacgct
     1261 cccgataaga tcgatccacg gctggcagat ctgctggcgc gaaagccgac gagggtcgct
     1321 accgtcgcaa tggcgaacaa gaccgcgcgg gtcatctggg cgatcatggc acgtggcgaa
     1381 acctatcgca ccggccacca gccggcattg gcagcataac aaccatacag gatccagctg
     1441 aagaggagat cagcgacacg aggttgcgag cgcgatgcga tgtgatggca gaccggtcag
     1501 accgggaaca gggacaaccc aagtgatgtc caaggcatca aagcctgata agcagaatgg
     1561 gaccctgttc gcggactcca tcagggccag cagtctcaaa cgactgcatc gacaggccgg
     1621 acagaagact gcacccgacc aatgccaata cgtcaattta gcccttgcaa tgcgggagcc
     1681 atccacagag gacattcatc ggggattccg ttcatttctc gttcgtcaaa acgcgagtca
     1741 atcgatgctg gtcagcgttg attcaagtca aggttcgtta ccagctcttt agttatcaat
//
LOCUS       DATLJT010001472         1886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1742597,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001472 DATLJT010000000
VERSION     DATLJT010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742597"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VM910_33790"
     CDS             complement(<1..191)
                     /locus_tag="VM910_33790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HVI67517.1"
                     /translation="MAKTKFRPLHDRVVVKRIDAEEKTKGGIIIPDTAKEKPSQGEIT
                     AVGPGGRDETGKLIPIDVKV"
     gene            673..1182
                     /locus_tag="VM910_33795"
     CDS             673..1182
                     /locus_tag="VM910_33795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67518.1"
                     /translation="MISLGYARTGVRLAVVSLLTAFLGACSSFSPSGSSPSVSSDPGA
                     GPEMPATIRADELVGRWGLASYQNPNDRARTEKQAQAQCKQPYVITAGTSGGVVMHLA
                     DQATPQELRIKGSQSGKNYIGPAGPPGGEQDREIVSFDGRVLVTRFIDKDAATRYGNM
                     VYVRCAPRA"
     gene            complement(1248..>1886)
                     /locus_tag="VM910_33800"
     CDS             complement(1248..>1886)
                     /locus_tag="VM910_33800"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HVI67519.1"
                     /translation="RAQMGMPGRSATGIEGQVAREAAEAHVLLETYRAQIEQCEKLKV
                     ELDLIHDAINRTKREIAVLHGKSFDGQEMAKVNGELGAVVGGTEQATQQILEAAESID
                     QAASALSRVNSPEQQKLLSEEIQERIVSIYEACNFQDLTGQRISKVMATMKFIESHIN
                     VMMEIWGGVDAIKAHAPAILDTREGDARLLNGPKLDGDAGHASQDDIDALFD"
BASE COUNT          308 a          577 c          595 g          406 t
ORIGIN      
        1 accttgacgt cgatcgggat cagcttgccg gtttcgtcgc ggccgcccgg gcccaccgcc
       61 gtgatctcgc cctgcgaggg tttttccttg gcggtatcgg ggatgatgat cccgcctttg
      121 gtcttttcct cggcgtcgat acgcttgacc acgacgcggt cgtgcagcgg acggaatttg
      181 gttttggcca tgagatttcc ttttggaggc tcgcttcgga tcggggctgt tcaacgaagg
      241 ccgatcggag tgctgccatc accggcaccg gggcggcgcc aaaaaaggcc aacatccttg
      301 gcaatcgtgc cttcggagtg ctaatcgagc gcccgaaata tggcttggcg ggggtcctgt
      361 caagcaacgg ggtttaaggc gttcttgacc ctgttttaga atggtgcagc acgaaaccgg
      421 atcgaacggt cgggcgtagt tgttgctccg acagcggttt tgggcttggc tgtaggacag
      481 gtgcggccgc tcatgcggcg gttgagaagt ccgcatcggt attggcgcga gcttgccatg
      541 cggacttctg aacaaggcca aacttagaat aggtttattc gtgtccttcg aacccgaagt
      601 tcgcaaaaga gcctgccgca cgatcgggcg aactttaggt gggggacagg ggtcttgttt
      661 cgggggaatg ccatgatcag tctgggctac gcgcgcacgg gtgttaggct tgccgttgtc
      721 tcgttgctga cggcctttct cggggcctgc tccagcttca gcccgtccgg ttcgtctccg
      781 tcggtttcgt ccgacccggg cgcgggcccc gaaatgccgg cgaccatccg cgccgatgaa
      841 ctggtcggca gatggggcct ggcgtcctat caaaatccga acgaccgcgc ccgcaccgag
      901 aagcaggcgc aggcgcagtg caagcaaccc tacgtcatca ctgccggaac ctcgggcggt
      961 gtggtcatgc atctcgccga ccaggcgacg ccccaggaac tcaggatcaa gggcagccag
     1021 agcggaaaga attacattgg tccggccgga ccgcccggcg gcgagcagga ccgcgagatc
     1081 gtgtcgttcg acggccgcgt gctggtcacc cgtttcatcg acaaggacgc cgcgacccgc
     1141 tacggcaaca tggtctatgt gcgctgcgcg ccgcgcgcgt aggaaagacg gtttaacaag
     1201 aacgccggca tgcgccggcg tctttcattt ccggtctgtt cgagccgtca gtcgaacagc
     1261 gcgtcgatat cgtcctgcga agcgtgaccg gcgtcgccgt cgagcttcgg tccgttcaaa
     1321 agccgcgcat cgccttcgcg cgtatcgagg atcgccggtg cgtgcgcctt gatcgcgtcg
     1381 acgccgcccc agatttccat catcacgttg atgtggctct cgatgaactt catcgtggcc
     1441 atgaccttgc tgatgcgctg cccggtcagg tcctgaaaat tgcaggcttc gtaaatcgag
     1501 acgatgcgtt cctggatttc ctcggacagg agcttctgct gctccggcga attcaccctg
     1561 gagagcgcgc ttgccgcctg atcgatcgat tcggcagcct cgaggatctg ctgggttgcc
     1621 tgctcggtgc cgccgaccac ggcgccgagc tcgccgttga ccttggccat ctcctggccg
     1681 tcaaagctct tgccgtgcag caccgcgatc tctcgcttgg tgcgattgat ggcgtcatgg
     1741 atcaggtcca gctcgacctt gagcttctcg cactgctcga tctgggcgcg ataggtctcc
     1801 agaagcacat gcgcttccgc ggcctcgcgc gctacctggc cttctatgcc ggttgcgctt
     1861 cttcccggca tgcccatctg cgcgcg
//
LOCUS       DATLJT010001473         1902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3810039,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001473 DATLJT010000000
VERSION     DATLJT010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3810039"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VM910_33805"
     CDS             <1..304
                     /locus_tag="VM910_33805"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67520.1"
                     /translation="LVNFNLDRIIKSSSAARLPTLFNVGEYVKAGGLISYGPSFLDMF
                     RRAAEYTDKILRGAKPGELPIEQPTKFELVINLATAKLLGITIPGTVIARADDVIE"
     gene            525..1517
                     /locus_tag="VM910_33810"
     CDS             525..1517
                     /locus_tag="VM910_33810"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67521.1"
                     /translation="MRRREFITLLGGAAAAWPLAARAQQAEHMRRVGVLVNSVEADPA
                     VQGRIAAFRQALERLGWLEGRNIHIELRFSAANFERLPQLAQEMVALNPDVIFASTTP
                     AIKALQATTHTIPIVFVYVSDPAGAGVVASLARPGGNTTGLLLYEESITGKWLGMLKE
                     ISPRLTRAALVGNPKGFTYAYFVRSSKAIAPALGIEVAPAPIENDAAAIEQRIEDFAR
                     APNGGLFVPPDITTDQHRDLIIALAARYRLPAVFAFRHFVTAGGLMSYGVEMLEQHRQ
                     AASYVDRILRGANPADLPVQAPTKYETVLNLKTAKALGFDVPPTLLVRADEVIE"
     gene            1705..>1902
                     /locus_tag="VM910_33815"
     CDS             1705..>1902
                     /locus_tag="VM910_33815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymethylenebutenolidase"
                     /protein_id="HVI67522.1"
                     /translation="MHRLIFALSVSLVASVAFAAPPNDMAARTELHAIDTLTLSDAQF
                     LTGDANARATVTTGVLRIPRGD"
BASE COUNT          364 a          591 c          566 g          381 t
ORIGIN      
        1 actcgtgaat ttcaacctgg accgcataat aaaatcgtcg agcgcggcgc gactgccaac
       61 gcttttcaat gtaggtgagt acgtcaaagc cggaggcttg atatcttatg gtccaagttt
      121 tttggacatg ttccggcgcg cggccgagta caccgacaag attcttcgcg gcgcgaaacc
      181 gggtgaatta ccgatcgagc aaccgacgaa gtttgaactt gtcatcaatc tcgcaaccgc
      241 gaagctgctg ggcataacca taccgggaac ggtcatcgcc cgcgccgacg acgtgatcga
      301 ataacaatat cttttgctgc ggcgcaggag tccgcttttg gcacaaagcg ggcatcttga
      361 agtgtccgct atttgtccgc tttcgggggt aaagcggaca tcgctccagt tacgacgcta
      421 cgtctgcttt tgacccaaac cggacatgag cgggccgctt ttgctgcgat gcacggccct
      481 gacctgctat actccccacg atcgttgggt ttgaggtcag cgcgatgagg cggcgcgagt
      541 tcatcacgct ccttggcggc gctgcagccg cgtggccgct cgcagcgcgc gcgcagcagg
      601 ccgagcacat gcggcgcgtc ggcgtgctag tgaacagcgt ggaagctgac ccagccgtcc
      661 aagggcggat cgcagcattc cggcaggcgc tcgaacggct cggatggttg gaaggccgaa
      721 acatccatat tgagttgcgt ttctctgccg ccaatttcga gcggctccca caattggcgc
      781 aggagatggt ggcgctcaat cccgatgtca tcttcgccag cacgacgcca gccatcaaag
      841 cgttgcaggc gaccacccac acgatcccga ttgtcttcgt atacgtgtcc gacccggccg
      901 gcgccggcgt cgtggccagt ctggcgcggc caggcgggaa caccaccggc ctactgttgt
      961 atgaggagag catcaccgga aaatggttgg ggatgctcaa ggagatttcg ccgcgtctga
     1021 cccgagcagc gctggtcggc aatccaaagg ggttcactta cgcctatttc gtgcgctcgt
     1081 cgaaggcgat cgcgcccgcg ctcggaattg aagtcgcgcc cgcgcctatc gagaatgatg
     1141 ccgccgccat cgaacagcgc atcgaggatt tcgcgcgtgc gccgaacggt ggtctcttcg
     1201 tgccaccgga catcacgacg gatcagcatc gcgatctcat catcgcgctc gcggcgcgat
     1261 accgtctgcc tgccgtgttt gctttccgcc acttcgtcac cgccggcggg ctgatgtcct
     1321 atggcgtcga gatgctcgag cagcatcgac aggcggcatc ctacgtcgat cgcatcctgc
     1381 gcggcgccaa tccggccgat cttcccgtgc aggcgccgac caagtacgaa acggtcctta
     1441 acttgaagac ggcgaaggcc ctcggcttcg acgtgccgcc gacgcttctg gtccgcgccg
     1501 acgaggtgat cgagtagact cactttattg cagtgcgttc ggtcgctatt ggcacaaagc
     1561 ggacaattcg taacgtctgc ctgtttgtct gcttttgggg gtaaagcggc tgcacgtcac
     1621 gaccggcggc ctttagccgc tcgacataca ccttgcacag ggctgctatc ctcggctcag
     1681 aacgaccgaa cgagggagta accgatgcac cggctcatct tcgcgctatc cgtatcttta
     1741 gtggcatcgg tcgcgttcgc cgcgccgccc aacgacatgg cggcgcgtac cgaactgcac
     1801 gccatcgaca cgctcacttt gtcggacgcg cagtttctga ccggcgacgc caacgcaagg
     1861 gcgacggtca ccaccggtgt attgcgcatt ccaaggggcg ac
//
LOCUS       DATLJT010001474         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1742781,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001474 DATLJT010000000
VERSION     DATLJT010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742781"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VM910_33820"
     CDS             <1..909
                     /locus_tag="VM910_33820"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVI67523.1"
                     /translation="GLQAIAVAARSVMLDGVEIAIGGGIESISLVQNEHMNTFHAVDE
                     ELMALKPEMYMSMLETAEVVAERYKIGRDKQDEYSLECQRRVGAALQGGRFNDEIVPI
                     TTKMAVVNKDTKEVSYQPVTLTKDEGPRPDTTAEGLAKIKPVFEGKTISAGNASQLSD
                     GASACVIMSDKIAAQKGLKPLGIFRGFVAAGVEPDEMGVGPVAAIPRLLKRHNLKIDD
                     IDLWELNEAYAVQVIYCRDKLGIDPDKLNVNGGSIAIGHPHGMTGSRLTGHLLIEGRR
                     RKAKYGVVTMCIGGGMGAAGLFEIVH"
     gene            931..1941
                     /locus_tag="VM910_33825"
     CDS             931..1941
                     /locus_tag="VM910_33825"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HVI67524.1"
                     /translation="MKTAITELFGIQHPIIQGGMHYVGFAELAAAVSNAGGLGIITGL
                     TQKTPERLAKEIARCRDMTNKPFGVNLTFLPSFSAPPYPEYIAAIREGGVKVVETAGR
                     SPEQYMPALKAAGIKVIHKCTSVRHSLRAEKIGCDAVSVDGFECGGHPGEDDIPNMIL
                     LPRAADELKVPFVASGGMADARSLVAALAMGAAGMNMGTRFIATKEAPVHPNVKKALV
                     EATELDTRLVMRALRNTERVLKNKGVDDLLEIEREKGATLRIEDIHDQVAGVYPKVMV
                     DGDMDAGAWSCGMVVGLINDIPTVKELVDRIMAEAERIIRQRLTGFLDGVEVQKPARA
                     VA"
     gene            1953..2723
                     /locus_tag="VM910_33830"
     CDS             1953..2723
                     /locus_tag="VM910_33830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI67525.1"
                     /translation="MTEHVKIEIADGIMTLTLRRPEKKNALTGAMYDALSDALTRAES
                     NTSVRVVLFQGDGDSFTAGNDLADFASQSRGESAVDSPAHRFIETISKVSKPLVAAVQ
                     GNAVGVGTTMLLHCDLVYLADNARLITPFVNLALVPEAASSWLLPLRIGHARAYAMFA
                     LGEPMDAAAALACGLANAVVPQAELRKKAHDTAVALTKRPAGSLSLTKKLMREHERIA
                     AQIAEEGQLFKERLTTPEAREAFAAFAERRPPDFGKVA"
     gene            2778..>3019
                     /locus_tag="VM910_33835"
     CDS             2778..>3019
                     /locus_tag="VM910_33835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI67526.1"
                     /translation="MAAALRIFSYLPNPRVWKATIAARFCGVDVEIRGAPPKELRDWI
                     WDYDARPLDEQERQSLLSLARTGRVGLAGAKLFKTDA"
BASE COUNT          599 a          903 c         1004 g          513 t
ORIGIN      
        1 ggcctgcagg cgatcgcggt cgccgcccgc tcggtgatgc tcgacggcgt cgagatcgcg
       61 atcggcggcg gcatcgagtc gatcagcctg gtgcagaacg agcacatgaa cacgttccac
      121 gccgtcgacg aagagctgat ggcgctgaaa ccggaaatgt acatgtccat gctcgagacc
      181 gccgaggtgg tcgccgagcg ctacaagatc ggtcgggaca agcaggacga atactcgctc
      241 gagtgccagc gtcgtgtcgg cgccgcgctg cagggcggcc gcttcaatga cgagatcgtg
      301 ccgatcacga ccaaaatggc cgtggtcaac aaggacacca aggaggtcag ctaccagccg
      361 gtgacgctga ccaaggacga aggcccgcgg cccgatacca cggccgaagg gctggcaaag
      421 atcaagccgg tgttcgaagg caagaccatc agcgccggca acgccagcca gctctcggac
      481 ggtgcctcag cctgcgtgat catgagcgac aagatcgctg cgcaaaaggg cctgaaaccg
      541 ctcggcatct tccgcggctt cgtggccgcc ggtgtcgagc cggatgagat gggcgttggt
      601 ccggtcgccg cgatcccgcg gcttctgaag cgccacaatt tgaagattga tgacatcgac
      661 ctctgggagc tcaacgaggc ctatgcggtg caggtgatct attgccgcga caagctcggc
      721 atcgatcccg acaagctcaa cgtcaacggc ggctcgatcg cgatcggtca tccccatggc
      781 atgaccggct cgcggctcac cggccacctc ctgatcgagg gtcggcggcg caaggcgaaa
      841 tacggcgtgg tgaccatgtg catcggcggc ggcatgggcg cggcgggtct gtttgaaatc
      901 gtccactgat cggaaacgca ggagatcttc gtgaagacag caatcactga actgttcggc
      961 atccagcatc cgatcatcca gggcggtatg cactatgtcg gcttcgccga gcttgcggcg
     1021 gcggtatcga atgccggcgg tctcggcatc atcaccggcc tgacccagaa gacgccggaa
     1081 cggctcgcaa aggagatcgc gcgctgccgc gacatgacca acaagccgtt cggtgtgaac
     1141 ctaaccttcc tgccgagttt cagtgcgccg ccctatccgg aatacatcgc ggccatcagg
     1201 gaaggcgggg tcaaggtcgt ggagacggcg ggccgcagtc ccgagcaata catgccggcg
     1261 ctgaaggcgg ccggcatcaa ggtaatccac aaatgcacct cggtacggca ctcgctgaga
     1321 gctgagaaga tcggctgcga cgccgtcagc gtcgacggct tcgagtgcgg cggccatcct
     1381 ggcgaggacg acatcccgaa catgatcctg ctgccccgcg cggccgatga gctgaaggtg
     1441 ccgttcgtgg cctccggcgg catggcggat gcgcgtagcc tggttgcggc gttggcgatg
     1501 ggcgcggccg gaatgaacat gggcacgcgt ttcatcgcca ccaaggaagc gccggttcat
     1561 cccaacgtga agaaggcgtt ggtggaggca acggagctcg acacccgatt ggtgatgcgt
     1621 gccttgcgca acactgagcg cgtgctcaag aacaagggtg tcgacgatct gctcgagatc
     1681 gagcgcgaga agggcgcgac gctgaggata gaggacatcc atgaccaggt tgcgggcgtc
     1741 tatccgaagg tcatggtcga cggtgatatg gacgcgggcg cctggagctg cggcatggtg
     1801 gtcggcctga tcaatgacat cccgacggtc aaggagttag tcgatcgcat catggcggag
     1861 gccgaacgga tcatccgcca gcgcctgacc ggcttcctcg acggcgtcga ggttcagaaa
     1921 cctgcccgcg cggtcgcttg acggagaaag ccatgaccga gcatgtcaaa atcgagatcg
     1981 ctgatggcat tatgacgctg accttgcggc gtccggagaa gaagaacgcg ttgacgggcg
     2041 ccatgtatga tgccttgtcc gatgcgctca cgcgggcgga gagtaatacg tccgtccgtg
     2101 tggtcctgtt ccaaggcgat ggcgacagct tcaccgccgg caatgatctc gccgacttcg
     2161 caagccagtc gcgcggcgaa agcgcggtcg acagcccggc ccaccgcttc atcgagacca
     2221 tcagcaaggt aagcaagcct ttggttgccg ccgtgcaggg caatgcagtc ggcgtcggca
     2281 cgaccatgct tctgcattgc gaccttgtct atctcgctga caacgcgcgg ctaatcacgc
     2341 cctttgtaaa tctcgcgcta gttcctgagg cggcgtcgag ctggctgttg ccgttacgga
     2401 taggtcacgc gcgcgcgtac gcgatgtttg cgcttggcga gccgatggat gcggccgctg
     2461 cgctggcatg cggcctcgcc aacgccgtcg tgccgcaggc agaactgcga aagaaggcgc
     2521 atgacacggc agttgcgctg accaaacgtc cggcaggttc gctcagccta accaagaagc
     2581 tgatgcgcga gcacgagcgg attgctgctc agatcgctga ggaagggcag ctgttcaagg
     2641 agcggttgac gactcccgaa gcacgcgagg catttgctgc attcgccgaa cggcggccgc
     2701 ctgacttcgg caaggtcgcc tgactttcgt ggacgtggaa gtcgttttca ggaagtctcg
     2761 cctaggagcg atcgaagatg gccgccgctt tacggatttt ctcttacctg cccaacccgc
     2821 gcgtgtggaa ggcgaccatc gccgcgcggt tctgcggcgt ggacgtcgag atcaggggag
     2881 ccccgcccaa ggaattgcgg gactggatct gggactatga cgcgcggccg cttgacgagc
     2941 aggaacgcca atcgctgctg tcgcttgccc ggaccgggcg ggtgggcctg gccggcgcaa
     3001 aactcttcaa gaccgatgc
//
LOCUS       DATLJT010001475         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1511273,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001475 DATLJT010000000
VERSION     DATLJT010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511273"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VM910_33840"
     CDS             complement(<1..182)
                     /locus_tag="VM910_33840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67527.1"
                     /translation="MKRRAFIMLLGAAVAWPLAARAQQPERMRRIGVLAGTTAESNSE
                     AQAVPAVFRQALQQLG"
     gene            complement(179..1171)
                     /locus_tag="VM910_33845"
     CDS             complement(179..1171)
                     /locus_tag="VM910_33845"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67528.1"
                     /translation="MTRRNFITLLGGAAAAWPLAVRAQQPERMKRIGVLMGVPESDAE
                     YQAFLAAFREGLQELGWAEDRNIRIDTRWAGFDVQSMQQNAKELIALQPALIVSQSTP
                     TTAALKQQTSTVPIVFVNVVDPVGSGFVASFPRPGGNVTGFILMEPTMAGKWLELLKE
                     IAPRVNRVVLLFNPATAPYAEYYLNPFRAAAASLAVEAIVAPVRDTSELESVVATQAR
                     EPNSGLIVMPSSFMNVHRTEIISLAARDRLPAVYAFRFFAELGGLLSYGNDFLNNYRR
                     AATYADRILKGEKPNDLPVQAPVKFQLVINLKTAKALGLEVPPTLLARADEVFE"
     gene            complement(1168..2151)
                     /locus_tag="VM910_33850"
     CDS             complement(1168..2151)
                     /locus_tag="VM910_33850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67529.1"
                     /translation="MKRRAFIMLLGAAVAWPLAAGAQQPAVPVIGLVNPGSREASASR
                     VAAFRKGLSEIGYVEGQNVAVEYHWLGGQYDRLPTLMADLVGRRVAVIATPGSTPAAS
                     AAKAATTTIPIVFAVGEDPVKLDLVSSLARPGGNATGTNVFLWEIAAKRLGLLHELVP
                     RAVRVAVLINPANVPSAEATLRDLPEAARAIGLQIQILNASTSGEIEAAFATLVRDRA
                     DVLFVAADSFFISRRIQFATLAARHGIPAACSTREEVEAGGLMSYGTDISDMWRQVGL
                     YAGQVLKGAKPADLPVVQSTKFEFVINLVTARALGLEVPPALLARADEVIE"
     gene            complement(2148..>2803)
                     /locus_tag="VM910_33855"
     CDS             complement(2148..>2803)
                     /locus_tag="VM910_33855"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67530.1"
                     /translation="RSVPIVFVLVVDPVSSGFVDSLARPGGNITGFAWAEYSIGGKWL
                     ELLKEIAPGMTRAAVIRDAALTAGGGQLGVIQAVAPSVRAMTPVNVRDAGEIERAITA
                     FARSPNGGLIVTGSTLAAVHRDLIVTLAARHKLPAIYFARYFVVGGGLISYGPGLVDQ
                     FRHAASYVDRVLKGEQPADLPVQAPVKYELAINLKTAKALGLEVPPTLLARADEVFE"
BASE COUNT          514 a          890 c          915 g          484 t
ORIGIN      
        1 caacccaatt gctgcaacgc ctgccggaat acggcaggaa cggcttgagc ttcggaattg
       61 gattcggcgg tagtacccgc gagcacaccg atccgccgca tccgctcggg ctgctgcgcg
      121 cgcgccgcga gcggccacgc cacggcggcg ccgaggagca tgatgaacgc gcgccgcttc
      181 actcgaacac ctcgtcggcg cgggcgagca gcgtcggcgg cacttcgagg ccgagtgcct
      241 ttgcggtctt gaggttgatg accagttgga acttgaccgg cgcctggacg ggaaggtcgt
      301 tgggcttttc gcccttcagg atgcgatcgg cataggtcgc cgcgcgtcga taattattga
      361 ggaaatcatt cccgtaggac agtaggccgc caagttcagc gaagaaacgg aacgcataga
      421 cggcagggag tcggtcgcga gcggccagcg atatgatctc cgtgcgatgg acgttcatga
      481 aggaacttgg catcacgatc aggccgctat tcggctcgcg cgcttgtgtg gcaacaacgg
      541 attccagctc ggacgtgtcg cgaacaggcg cgacgatcgc ttccaccgca agggacgcag
      601 ccgcagctct gaaggggttc agataatact cggcgtaggg cgcggttgcc ggattaaaca
      661 ggaggactac ccggttaacg cgtggagcaa tctccttaag cagctccagc cacttgccag
      721 ccattgttgg ctccataagg atgaaaccgg tgacgttgcc accgggtcgc gggaagctcg
      781 cgacgaagcc gctgcccacc ggatcgacaa cgtttacgaa gacgatcggg acggtgctgg
      841 tttgttgctt taatgccgcc gtggtaggtg tgctttgcga aacaatcagg gcgggctgca
      901 gcgcgatgag ttcctttgcg ttttgctgca tcgactgcac gtcgaagccc gcccagcgag
      961 tatcgatccg gatgttgcgg tcctccgccc atcccagttc ctggaggcct tcccggaaag
     1021 cggcgagaaa ggcttgatat tccgcgtcgc tctcgggcac ccccataagc acgccgatgc
     1081 gcttcatccg ttccggctgc tgcgcgcgca ctgccagcgg ccacgccgcc gcggcgccgc
     1141 cgagcagcgt gatgaagttg cgccgcgtca ttcgatcact tcgtcggcgc gggcgagcag
     1201 cgccggtgga acttcgagtc cgagcgctcg cgcggtcaca agattgatga cgaactcgaa
     1261 cttggtggac tgcacgaccg gcaggtcggc aggcttggcg cctttgagga cttgaccggc
     1321 atagaggccg acctgccgcc acatgtccga aatatcggtc ccatagctca tcagcccgcc
     1381 ggcttcgacc tcctcgcgag tggaacaggc cgcgggaatc ccgtggcgcg ccgcaagcgt
     1441 cgcaaactgg atgcgccggc tgatgaagaa cgagtcggcg gcgacgaaca ggacatcggc
     1501 ccggtcgcgc acgagggtgg cgaatgctgc ctcgatctcg ccgctggtgc tggcgttgag
     1561 gatctgaatt tgcagtccga tcgcgcgagc agcttccggc aggtctcgca aggtggcctc
     1621 ggcgctcggt acgttggccg gattgatcag cacggcaacg cgaacggccc tgggcaccag
     1681 ctcgtgcagc agacctagcc gcttggccgc gatctcccag aggaaaacgt tggtacccgt
     1741 cgcattgccg cccggccggg caaggcttga caccagatcg agcttcacag gatcttcgcc
     1801 gaccgcgaac acgatcggga tcgtcgtggt cgcggctttg gccgcactcg cagcaggcgt
     1861 gctaccgggc gtggcgatta ctgctacgcg gcggccgacg aggtcggcca tcagcgtcgg
     1921 cagccggtcg tattgaccgc ccaaccagtg gtactccacc gccacgtttt ggccttcgac
     1981 gtagccgatt tcgctgaggc ctttgcggaa ggcggctacc cgggacgctg acgcctcgcg
     2041 cgagccggga ttgacaaggc cgatgactgg gacagccggc tgctgcgcgc ccgccgcgag
     2101 cggccacgcc acggcggcgc cgaggagcat gatgaacgcg cgccgcttca ctcgaacacc
     2161 tcgtcggcgc gggcgagcag cgtcggcggc acttcaaggc cgagcgcctt ggcggtcttg
     2221 aggttgatcg ccagttcata cttgaccggc gcctgcaccg gcaggtcggc cggctgctcg
     2281 cccttgagga cgcgatccac gtagcttgcc gcgtgccgaa actggtcgac gagtccaggc
     2341 ccataggaga tcaagccgcc gccaacgacg aagtagcgtg cgaagtaaat cgcgggcaat
     2401 ttgtgtcggg ccgccagcgt gacaatcaaa tcgcgatgaa ccgccgccaa agtgcttccc
     2461 gttacgatca ggccgccatt aggggaacgc gcgaaggccg tgatagcgcg ctcgatctcg
     2521 ccggcgtcac gcacattgac cggggtcatt gcccggaccg atggcgctac ggcctggatg
     2581 acacccaact ggccaccccc cgcggttaat gcggcgtccc gaatgactgc cgcccgtgtc
     2641 atgcccggtg cgatctcttt gagcagctcc agccattttc cgccgatgct gtattcggcc
     2701 catgcaaagc cggtgatatt gccgcccggc cgcgcgagac tatcgacgaa gccgctgctg
     2761 accggatcaa cgaccagcac gaacacgatt ggcacgctgc ggg
//
LOCUS       DATLJT010001476         2682 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3348840,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001476 DATLJT010000000
VERSION     DATLJT010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3348840"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..477)
                     /locus_tag="VM910_33860"
     CDS             complement(337..477)
                     /locus_tag="VM910_33860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67531.1"
                     /translation="MDWRDAMPRTEQVRATPEDETAYAAGWTISGLATIGTIIAVWVF
                     AI"
     gene            664..867
                     /locus_tag="VM910_33865"
     CDS             664..867
                     /locus_tag="VM910_33865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67532.1"
                     /translation="MRKLIIATAALAFLSSTAFAQDKGAATPPSGSDSMTKGDMTKDT
                     MSKSKKKTKSAKKKTGDDTMTKQ"
     gene            complement(1004..1519)
                     /locus_tag="VM910_33870"
     CDS             complement(1004..1519)
                     /locus_tag="VM910_33870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67533.1"
                     /translation="MQTELNKVIAALTEFFTREAFLLDKDCGERALTHRLAVHLERQF
                     SGWDVDCEYDRLGERTLRLPRGTIVSTGDHLAKSVFPDIVVHQREIPNNLVAIELRKD
                     SNHQPIEHDRHKLVALTDPHIWFAYWIGLLLILSRNNVTGCEIYVAGNLDRPMTVWFA
                     EQLKLAGLGGG"
     gene            complement(1588..>2682)
                     /locus_tag="VM910_33875"
     CDS             complement(1588..>2682)
                     /locus_tag="VM910_33875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HVI67534.1"
                     /translation="QIATLILPADTAWNEADGIAQVPPPGQKPGFSTEAVETAAKVLH
                     GDAAHTLLLVTGSALTEQGLALAQQISGKTGCRVMGQTYHPRMARGRGRFSIDRIPYV
                     IELALPILKDFKHIVLVEANDPVAFFAYPNKPSHLKPAGCEVHRLTAWGENSVAALEA
                     LADALSAKPHDVQPQKLVELNKPTGPLTYPSIAQAIACAIPENAIIVDESITTGRGFF
                     PPTAAAAPHDWLQNMGGSIGFSTPVATGAAVACPDRKVICMVGDGSAMYTIQSLWTQA
                     REGLDVVTIVFANRIYQILRGEFDGVGAGEPGQRALDMLKIDRPTIDFVSLARGMGVE
                     ARAVTSADELNKALAEAIAEPGPRLIEVQM"
BASE COUNT          503 a          862 c          825 g          492 t
ORIGIN      
        1 tgcaccgcaa ggtctttttc gattcggcgt atggtctgct atggtcgcct tggagccgtg
       61 gttcttcctt cacacgggag aggtccaagg ttcgatccct tgtgcgccca ccatgcttcg
      121 cccttcgggc tacgcatggc actgccagcc aggaagctta cggcgaagca tgtccgccgt
      181 agcttgagca aagcgagagc gaagacggac agagaaaggc tggccgctga agcctttggc
      241 gaaggccggc ttcctccgcg agcttctgct ggcaagccac tttcgccctt gcgggctgcg
      301 cccggcgcaa ccggccaagg ccatcggcgc gaagcgtcag attgcaaaca cccagaccgc
      361 aatgatggtg ccgatcgtcg caagaccgct gatggtccag ccggcggcat aagcggtttc
      421 gtcttcgggt gtagcgcgca cctgttcggt ccgcggcatc gcatctcgcc agtccatgac
      481 ctttctccga aaaggcctgc caccaacgaa taatccgcgc ggccatcgcc ctgttccggc
      541 gcatttgcgg aaaaaggccg attccatgac ttgcgctctc acgaataact gatcagcgcg
      601 gcggaaccgg aatcgccgag gttccgttat ggtgcgccag ccaagaccaa cggaggtttc
      661 cgcatgcgca aactgatcat cgctaccgct gctctcgcct ttctctcttc gacagctttt
      721 gctcaagaca agggcgccgc cacgccgccg tccggcagcg acagcatgac caagggcgac
      781 atgaccaagg acacgatgag caagtccaag aaaaagacga agtccgccaa gaagaagacc
      841 ggcgacgaca ccatgaccaa gcaataggac accaagtcac aagaccgagt ccgcaaagca
      901 tgactggccg cccggtgggg cggcctttcc ttttcaagtg gatcgcgcgc tacttcgcgg
      961 cctgttcgca ctgccgcctt gtgaagatgc aagcctccac tctctagccg ccgcccaacc
     1021 cagccagctt cagttgctcc gcgaaccaga cggtcatcgg acggtcgagg ttacccgcca
     1081 catagatctc gcagccggtc acgttgtttc tggacaggat caaaagcagg ccgatccaat
     1141 aggcgaacca aatatgcgga tcggtgaggg ccacgagctt gtgccggtcg tgctcgattg
     1201 gctggtggtt cgaatccttg cggagttcga tcgccacgag attgttcgga atctcgcgct
     1261 gatgcacgac gatgtcggga aataccgatt ttgccaggtg atcgccggtc gagacgatcg
     1321 tgccgcgcgg cagccgcagc gtgcgttcac cgagccggtc gtactcgcaa tcgacgtccc
     1381 agcccgaaaa ctgcctttcg agatgaaccg ccagacgatg cgtcagcgcg cgctcgccgc
     1441 aatccttgtc gagcagaaag gcctcgcgcg tgaaaaactc ggtcagcgca gcgatgacct
     1501 tgttcagctc ggtctgcatc ctgcctccga gaggcctgtc atcgggaccg gcattcgcgg
     1561 acccagcggc tcgcaatgtc ccggccccta catctggact tcgatcagcc gcggcccggg
     1621 ctcggcaatc gcttccgcca gcgccttgtt gagttcgtcc gcgctggtca ccgcgcgtgc
     1681 ttccacgccc atgcccctgg cgagcgagac gaaatcgatg gtcggacgat cgatcttcag
     1741 catgtccagc gcgcgctgtc cgggctcgcc ggcgccgacg ccgtcgaact cgccgcgcag
     1801 aatttgataa atgcggttgg cgaacacgat ggtgacgacg tcgagaccct cgcgcgcctg
     1861 cgtccacagg gactggatcg tgtacatggc gctgccgtcg ccgaccatgc agatcacctt
     1921 gcgatccggg catgcgaccg ccgccccggt tgcaacgggc gtggaaaagc cgatcgagcc
     1981 gcccatgttc tgcagccagt cgtgcggggc ggccgcggcc gtgggcggga agaagcctct
     2041 gccggtcgtg atggactcat cgacgatgat cgcattctcg gggattgcgc aggcaatcgc
     2101 ttgtgcgatc gagggatagg tcaagggccc ggtcggcttg ttcagttcga cgagcttctg
     2161 cggttgcacg tcgtgcggct tggcggagag cgcatccgcc agcgcttcca gcgccgcgac
     2221 cgagttttca ccccacgcgg tcagtcggtg cacctcgcag cctgccggct tcaaatggct
     2281 cggcttgttc ggataggcga agaaggcgac gggatcgttg gcctcgacca gcacgatgtg
     2341 cttgaaatcc ttgaggatcg gcagcgccag ctcgatcacg taaggaatcc ggtcgatcga
     2401 gaaacggccg cggccgcgcg ccatgcgtgg atgataggtc tggcccatca cccggcagcc
     2461 ggtcttgccg gagatctgct gcgccaacgc cagtccctgt tcggtgagcg cgctgccggt
     2521 gacgagcaga agggtatgcg cggcatcgcc atgcaacacc ttggccgccg tctcgacagc
     2581 ttccgtggaa aagcccggct tctggcccgg cggcggcacc tgcgcgattc catcggcctc
     2641 gttccacgcg gtatcggccg gcaggatcag ggtagcgatc tg
//
LOCUS       DATLJT010001477         6240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1511448,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001477 DATLJT010000000
VERSION     DATLJT010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511448"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VM910_33880"
     CDS             complement(<1..594)
                     /locus_tag="VM910_33880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HVI67535.1"
                     /translation="MALSKEEIRVAAESLHRAEKTRTQIRQFSLEYPAITVEDAYAIQ
                     KNWIEIKTAEGRTVRGHKIGLTSKAMQSALNIDEPDSGILLDDMFFTDGGVVPSDRFI
                     ATRVEAELAFVISSRLAGPGCTLFDVLNATDFVVPALEILDTRIERVDPVTKATRKIY
                     DTIADNAANAGIVLGGRPIRPLEADLRWIGALCFRNGQ"
     gene            788..1252
                     /gene="hpaR"
                     /locus_tag="VM910_33885"
     CDS             788..1252
                     /gene="hpaR"
                     /locus_tag="VM910_33885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HVI67536.1"
                     /translation="MREFSRSLPMSLLRAREAVMRQFRPSLRQHGLTEQQWRILRALA
                     AIKAIEVTALARTAFLLGPSLSRILRDLEARRLIERKTAKTDLRRGVVSISEKGLRLM
                     EVVAPSSEAIYAAITRRYGAKKLAELQEMLGALEASLSGLEVAGEREAEGYE"
     gene            1414..2499
                     /locus_tag="VM910_33890"
     CDS             1414..2499
                     /locus_tag="VM910_33890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HVI67537.1"
                     /translation="MVIIKLENLIDFVADVFAHADSSKEEARRIATYLTTANLTGHDS
                     HGVIRVPVYIRWKTMGSVVPNQTPEVVVDTPSLAVVDGKFGYGQTVTPQAVRIGIEKC
                     KKVGLAAVALRNAGHIGRVGDWAEMAAAEGLVSVHFVNAAGSLLVAPYGGVEKRLSTA
                     PYCVGIPREGQDPIVLDFATSIVAEGKVLVASRGGKKLPKGALIDADGSLSEDPAVLY
                     GPYTPDGPRDHAKGTGAIRAFGEHKGSGLAFICELLGGALTGTGATAPNRRFANGMLA
                     FYIDPDVVDPANFFDGEIARYVDFIRATKPIAGVEQVLIPGDPERKMRAERTRNGIPL
                     PDDTWAAIVNTAREVGVSEVSIQRATS"
     gene            2536..3312
                     /locus_tag="VM910_33895"
     CDS             2536..3312
                     /locus_tag="VM910_33895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HVI67538.1"
                     /translation="MPNKVKQIWASGKTVVNAWLAIPSGFSAEMIAQCGFDSVTVDMQ
                     HGVQDYLSMVQCFQAMHGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTPQEAK
                     NLVAYSKYPPKGVRSNGPIRAGMYGTAGTYQKAANDEIVLLPMMETKTAVESMEAILD
                     VEGIDGVYIGPSDLGFSYGLVPKLDREEPEILKIYEKIVKECGKRGLNPGIHCSGADG
                     AVRAINMGFKLVTLSNEVGLMSTYARMQVNQTREKSGGKA"
     gene            3410..4933
                     /locus_tag="VM910_33900"
     CDS             3410..4933
                     /locus_tag="VM910_33900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HVI67539.1"
                     /translation="MTPAPVIRLHPDDGVLIARSSLPPGIVVADGVTTVERIPAGHKV
                     AIKSIAAGEPVRRYGQIIGFATQPIAPGQHVHTQNCGMGDFAKDYAFGVDVKPVPNFD
                     LPATFDGIRRPDGRVATRNYIGILTSVNCSAHVAGLVADVFKKNPFTGDNPLADFPHV
                     DGVVALTHKTGCGMTQDEPLKLLRRTLGGYARHVNFSAVVVLGLGCEVNQIGGLMEEQ
                     KLAGRLRSMDIQEKGGTRKTVEAGIAFVKEALTDANKVKREPVPASELIVALQCGGSD
                     GYSGVSANPALGAASDLLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVSLMR
                     WWEDYTVREGAEMNANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVLHYAEPVTKK
                     GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNTPMYKRMED
                     DMDVNCGTILEGEETVQQCGQRIFDLMLKTASGQPTKSESFDFGGAEFAPWVLGATM"
     gene            5085..>6240
                     /locus_tag="VM910_33905"
     CDS             5085..>6240
                     /locus_tag="VM910_33905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI67540.1"
                     /translation="MSIYVALHHVTHYRYDRPIDLGPQTIRLRPAPHTRTPILSYSLK
                     VTPSNHFVNWQQDPQGNWLARYVFPEKATELKIEVDFTAQMTVVNPFDFFVEPYANSF
                     PFQYTEVLKAELAPYLEAIELGPLFAAYLRSIPREADLTVNFLVELNANLQKKIKYII
                     RMEPGVQTPEETLSASAGSCRDSAWLLIQTLRHLGLAARFVSGYLIQLRPDIDPIEAP
                     REVENDFTDLHAWAEVYLPGAGWIGFDVTSGMLTGEGHIPVAATPHYRSAAPISGTAG
                     HANVDFSYEMSVKRIREAPRITKPFSDQAWAELDRLGERIEADLKAQDVRLTMGGEPT
                     FVSIDDMESPEWNIAAVGPTKRGLADELIRRLRSRFAPGGLLHYGQGKWYP"
BASE COUNT         1211 a         1880 c         1968 g         1181 t
ORIGIN      
        1 ttgcccgttg cggaaacaga gcgcgccgat ccagcgcaga tcggcctcga gcggacggat
       61 cggccgtccg ccgagcacga tgccggcatt cgccgcgttg tcggcgatgg tgtcgtaaat
      121 tttccgcgtc gccttggtga ctggatcgac gcgctcgatg cgcgtgtcta ggatttccag
      181 tgccggaacg acaaaatccg tcgcattcag tacatcgaac agtgtgcagc cgggtccggc
      241 aaggcggctg ctgatgacga aggcgagttc cgcttccacg cgcgtggcga tgaaacggtc
      301 cgacggcacg accccgccat cggtgaaaaa catgtcatcg agcagaatcc cggagtctgg
      361 ttcgtcgatg ttgagcgcgc tctgcatcgc cttcgaggtc aggccgatct tgtggcctct
      421 cactgtgcgc ccttcggccg tcttgatctc gatccagttc ttctggatcg cataggcgtc
      481 ctcaacggtg atcgcgggat attcgagcga gaactgtcgt atctgggtgc gcgttttctc
      541 ggcccggtgc aggctttcgg cggcgacgcg aatctcttcc ttggaaaggg ccatgactac
      601 gcaaaaagca gatgacgggg atttgcttaa catgttaagt gaagccgatc aaaccgaatg
      661 acgcttgctg cggtgcaaac ttttcgtgat gacacgagcg atgaaaatgg cgcgatcggc
      721 caaaggtttg aacaagggtt tgagcaaggg ttccaattcc gccgcggcgc ctgcgcggcg
      781 cctaccgatg cgcgagttct cccgctcgtt gccaatgtcg ctgctccgtg cacgcgaagc
      841 ggtgatgcgg cagttccgac cctcgctgcg ccagcatggg ctgaccgagc agcaatggcg
      901 cattcttcgc gcgctggcgg cgatcaaggc catcgaggtc accgcgcttg cgcgcactgc
      961 ctttcttttg ggcccgagcc tgtcgcgcat cctgcgcgat cttgaagcgc gccgcctgat
     1021 cgagcgcaag accgcaaaga ccgatctgcg gcgcggcgtg gtgtcgattt ccgagaaggg
     1081 attgaggctg atggaggtgg tcgcgccatc gtcggaggcg atttatgccg cgatcacccg
     1141 gcgctatggc gcgaaaaaac tcgcggaact gcaggagatg cttggtgctc tggaggcgag
     1201 cctttcaggg ctcgaagtcg ccggcgagcg agaggccgaa ggttacgagt gactacaatt
     1261 ttgcatgatg ctacccggct tcatgccatg gacatgcgcg cgattattcg ctcaaagtgc
     1321 cgaccaagct gatgatcaaa atcagctacg gagacgggaa cgctgcactg atcccctcgg
     1381 tgcattgaca agtcagaggc tcaaaacctg cacatggtca tcatcaagct tgaaaacctc
     1441 atcgatttcg ttgcggacgt ttttgcccat gcggactcct cgaaggagga ggcgcggcgg
     1501 atcgcgacct atctcacgac cgccaatttg accggtcatg acagccacgg cgtgatccgg
     1561 gttcccgtct atatccgctg gaagacaatg ggctcggtgg tgccgaacca gaccccggag
     1621 gtcgtcgtgg atacgccctc tcttgcggtg gttgatggca agttcggtta cgggcaaacg
     1681 gtaactccgc aggccgtcag gatcggcatc gagaaatgca agaaggtggg ccttgccgcg
     1741 gtcgcattgc gcaacgccgg ccatatcggg cgcgtcggtg actgggccga gatggcggcg
     1801 gccgagggcc tggtctcggt gcatttcgtc aacgccgcgg gctctttgct ggtggcgcct
     1861 tacggcggcg tcgagaagcg gctgtcgacc gcaccttatt gtgtcggcat tccacgagag
     1921 gggcaggatc cgatcgtgct cgatttcgcg acctcgatcg ttgcggaagg caaggttctg
     1981 gttgcaagcc gcggcggcaa gaagctgccg aagggcgcgc tgatcgatgc cgacggcagc
     2041 ttgagtgaag atccggccgt gctgtacggg ccgtatacgc ctgacggacc gcgcgaccat
     2101 gccaagggca ccggggcgat ccgggctttc ggcgagcaca agggatcggg gcttgcgttc
     2161 atctgcgaat tgctcggcgg cgccttgacc ggaactggtg cgaccgcgcc gaaccggcgg
     2221 ttcgccaacg gtatgctggc gttctatatc gaccctgatg tcgtcgaccc cgccaacttc
     2281 ttcgacggcg agatcgcgcg ttacgtcgat ttcatccggg caaccaagcc gattgcaggt
     2341 gtcgaacagg tgctgatccc tggcgatccc gagcgcaaaa tgcgcgccga gcgcaccagg
     2401 aatggcatcc ccttgcctga cgatacctgg gcggcgatag tgaacaccgc gcgcgaggtc
     2461 ggtgtcagcg aagtgagcat tcagcgggcc acgagctaat tccactccag cgaaacggca
     2521 aaggaaacgc aatcaatgcc aaacaaagtc aagcagatct gggcctctgg caaaaccgtc
     2581 gtgaacgcct ggcttgcgat cccgtcaggc ttttccgccg agatgatcgc gcaatgcggc
     2641 ttcgacagcg tgaccgtcga catgcagcat ggcgtgcagg actatctgtc catggtgcaa
     2701 tgcttccagg caatgcacgg ccatccggtg acgccgatgg tgcgcgtgcc ctggaacgag
     2761 ccgggcatca tcggcaaggt gctggacggc ggcgcctatg gcgtgatttg cccgatggtc
     2821 aacacgccac aggaagcgaa gaatctcgtc gcatattcca aatatccgcc gaagggcgtg
     2881 cgttccaacg gcccgatccg cgccggcatg tacggcactg ccggcaccta ccagaaggct
     2941 gcgaatgacg agatcgtgct gctgccgatg atggagacca agaccgcggt agagagcatg
     3001 gaagcgatcc tcgatgtcga aggcatcgac ggggtctata tcggcccctc cgatctcggc
     3061 ttctcctatg ggctcgtgcc gaagctcgac cgtgaggagc cggagattct gaagatctac
     3121 gagaagatcg tgaaggagtg cggcaagcgc ggcttaaacc ccggcattca ctgcagcggc
     3181 gccgacggcg cggtacgcgc catcaatatg ggcttcaagc tcgtgacgct ctcgaatgaa
     3241 gttggattga tgtcgaccta cgcaaggatg caggtcaacc agacgcggga aaaatccggc
     3301 ggcaaggcgt gattttgtct tctccctctc cactgctctt gcggggagag gttggtcaaa
     3361 ggcgatgcga cctctccgta ggggaggtga agcgacgaag gaggctccca tgaccccggc
     3421 acccgtcatc cgcctgcatc ccgacgacgg cgtgctgatc gcgcgctcga gcctgccgcc
     3481 cggcatagtg gtggcggatg gggttaccac ggtcgaacgg ataccggcgg gccacaaggt
     3541 tgcgatcaag tcgattgcgg caggcgaacc tgtccgccgc tacggccaga tcatcgggtt
     3601 tgcgacgcag ccgattgcgc cgggtcagca cgtacatacg cagaactgtg gcatgggcga
     3661 tttcgcaaag gattacgcct tcggcgtcga cgtgaagccg gtgccgaatt tcgatctgcc
     3721 cgcgacattc gatggtatcc gccgccccga cgggcgcgtg gcgacgcgca actatatcgg
     3781 catcctcacc tcggtgaatt gcagcgcgca tgttgccgga ctcgtcgctg atgtcttcaa
     3841 aaagaatccg ttcaccggcg acaatccgct cgccgatttt ccccatgtcg atggggtggt
     3901 cgcgctcaca cacaagaccg gctgtggcat gacgcaggac gaaccgctga agctgcttcg
     3961 ccgcacgctc ggcggctatg cgcggcatgt gaatttctcc gccgtggtcg tgctcggctt
     4021 gggctgcgag gtcaaccaga tcggcggcct gatggaagag cagaagctcg ccggccggtt
     4081 gcgttcgatg gacattcagg aaaagggcgg cacgcgcaag accgtggaag ccggtattgc
     4141 cttcgtgaag gaggcattga ccgacgcgaa caaggtgaaa cgcgagccgg ttccggcaag
     4201 cgaattgatc gtggcgctgc aatgcggcgg ctcggatggc tattccggcg tctcagccaa
     4261 tccggcgctg ggcgcggcca gcgatctttt ggtccggcac ggcggcaccg tgattctttc
     4321 cgagaccccg gaaacctatg gtgccgaaca tctgctgacg cgacgcgcgg tcagccgcga
     4381 ggtcggcgag aaactggtct ccctgatgcg ctggtgggag gactataccg tgcgcgaggg
     4441 cgcggagatg aacgccaatc caagcccggg caacaaggcc ggcgggctca ccaccattct
     4501 cgaaaaatcg ctgggcgcga tggcgaaggc gggcagcacc aatctggtcg atgttctgca
     4561 ctacgccgaa cccgtgacca agaagggctt cgtctttatg gatacgcccg gctacgaccc
     4621 ggttgccgcg accggtcagg tcgcaggcgg cgccaatctc gtctgcttca ccacgggtcg
     4681 cgggagcgtg ttcggctgca agcccgcgcc ctcgatcaag cttgccacca acacgccgat
     4741 gtacaagcgc atggaagatg acatggacgt caattgcggc accatcctcg aaggcgagga
     4801 gacggtacag caatgcggcc agcgcatctt cgacctcatg ctgaagacgg cctcgggcca
     4861 gccgaccaag agcgagagct ttgatttcgg cggcgccgag ttcgcgccct gggtgctggg
     4921 cgcgacgatg tagctttccc tcgcgcgctg ccgcgacggc cgcccaaccg ggaccggaca
     4981 gcgaaatttc gctcgccgca taggcatctg ctgcccgttg ttaatgcttg atccgcgccg
     5041 ggacaggtgt tctgcttaaa aaagctgctg gagtagcccg tcccgtgtcg atctacgtcg
     5101 cactgcatca cgtcacgcat tacagatacg accgtccgat cgacctcggt ccacaaacca
     5161 ttcgcctgcg acccgcgccg cacacgcgta ccccgatcct gtcctattcg ctcaaggtca
     5221 cgccctcgaa ccatttcgtg aactggcaac aggacccgca gggcaactgg ctcgcacgtt
     5281 atgtcttccc cgagaaagcg accgaactga aaatcgaggt cgacttcacg gcgcagatga
     5341 cggtggtcaa tccgttcgat ttcttcgtcg agccctatgc caattccttt ccatttcagt
     5401 acaccgaggt gctgaaggcg gagcttgcgc cttatctcga ggccatcgag ctcggaccgt
     5461 tgttcgccgc ttatttgagg tcgatccccc gcgaggcgga tttgaccgtc aattttctgg
     5521 tcgagctgaa tgccaatctg cagaaaaaga tcaaatacat cattcgcatg gagcccgggg
     5581 ttcagacgcc ggaggagacg ctgtccgcgt ccgccggatc ctgccgcgac tcggcgtggc
     5641 tattgatcca gaccttgcgg catctcggcc tcgcggcgcg tttcgtctcc ggttacctga
     5701 tccagcttcg tcccgatatc gacccgatcg aggcaccacg cgaagtcgaa aacgatttta
     5761 ccgacctgca cgcctgggcc gaggtctatc ttccaggagc tggctggatc ggctttgacg
     5821 tgacctcggg catgctgaca ggcgagggac atatccccgt cgccgcgacg ccgcattacc
     5881 gttcggcggc accgatctcc ggcacggccg gacatgccaa tgtcgacttc tcttacgaga
     5941 tgagcgtcaa gcgcatccgc gaagcgccac ggatcacgaa gcctttttcc gaccaggcgt
     6001 gggccgaact cgaccggctc ggcgagcgga tcgaggccga tctcaaggcg caggacgtgc
     6061 ggctgaccat gggaggtgag cccacctttg tgtccatcga cgacatggag tcgccggagt
     6121 ggaatatcgc agccgtcgga ccaaccaaac gcggccttgc cgatgaactg atccggcgcc
     6181 tgcgcagccg ctttgcgccc ggcgggctgc tgcattacgg gcagggcaag tggtaccccg
//
LOCUS       DATLJT010001478         6191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1051176,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001478 DATLJT010000000
VERSION     DATLJT010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051176"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VM910_33910"
     CDS             complement(<1..615)
                     /locus_tag="VM910_33910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI67541.1"
                     /translation="MTISDKDESSLTGCFAGLRVLDFSTTIAGPHCARMLADMGAEVI
                     KIETAEGETMRTRPPVRNGCSTTFGQLNVGKKSVVLDLKSPEGREVVRRLVATVDILV
                     ENFRPGVMHRLRLDYETLSPLNPRLIYCSISGYGQTGPSAELPAYAPVIHAASGYELA
                     HLAYQPGRERPDYCGIYHADVLTGTYAFGAISAALYQRHQTQRGQ"
     gene            791..2206
                     /gene="glgA"
                     /locus_tag="VM910_33915"
     CDS             791..2206
                     /gene="glgA"
                     /locus_tag="VM910_33915"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HVI67542.1"
                     /translation="MKVLFVTTEMEDFVRVGGLAAVSAALPRALRPWADTRILLPGYA
                     DVVEQLTHIQIVGECAARAEMPPCRLGRSATRDGLPVYVLLCPQLYDRPGNPYGDESG
                     RDWPDNDIRFGRLASAAAELAAGTLDKNWAADLVHANDWQAALVPAYLAWSGAKVPSI
                     LTIHNLAYQGLFPRESLRRIGAPESAFHIDGVEFYDKLSFLKGGIVYASHLTTVSATY
                     AREITTPELGCGLEGLLRMRSDASQLTGILNGIDESWDPRFCAQLAQPFGAGDWEGKH
                     ANAEYVRKQFGLAVSRGPMFALVARLVPQKGIDLVLSAADEIIDACGQIVVTGSGEPA
                     FEEALVDAHRRRPKAFGVAIGFNDGQARRIFAGSDFTLMPSRFEQCGLSQMYAQRFGS
                     LPIGHQTGGLAETITDGETGFLFPQPSIESFLGGVRRAFSTFVSKDRLDSMRRSAMAR
                     TFSWDLSAACYSALYRKTVSP"
     gene            complement(2271..3500)
                     /locus_tag="VM910_33920"
     CDS             complement(2271..3500)
                     /locus_tag="VM910_33920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67543.1"
                     /translation="MPAPSQQSLRGLDWFIFFLADVQTGFGPFIAVYLTTQKWTQAEI
                     GLVLSIGSIVALIGQMPGGAIVDAARSERLVAGLAVATIGGAALCYATWPIFPVVVAA
                     ATLHAAASCVLGPAIAAISLGLVGPLKISERLGRNARFASLGNGVAAAVMGTCGYLLS
                     SRSVFLVTFALAIPTLLSLARIREDEVDIARAHGAVQRETADPRTTSVVALVCRRPLL
                     IFAGCILLLQLSNAAMLPLMAGIVTTRSSQWAPVLIAVCIVVPQAIVALMSPSVGRKA
                     QAWGRRPLLLIGFAALALRGVLFATVRDPYLLVAVQLLDGITASVFSVLVPLTVADIA
                     FGSGHFNLAQGIVGTATGIGASLSTVLAGFASDTFGSNVAFLGLAAVAALGLGVISLL
                     MPETRRSDGSDPATIVV"
     gene            complement(3617..4093)
                     /locus_tag="VM910_33925"
     CDS             complement(3617..4093)
                     /locus_tag="VM910_33925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HVI67544.1"
                     /translation="MRVRGWFILAIVLAVAGAAAWGAENDSQKNPPAATTQEPAPPPP
                     PSVTIIEPREAHGVLGRDVRSPANEDMGHIVDVIVDRSGTVRAAVIDFGGFLGVGSRK
                     IVVDWNALHFGRIADKRDSITLELTKAQVAAAPEYKEDASIVVLGASGSLQPLEDR"
     gene            complement(4124..>6191)
                     /locus_tag="VM910_33930"
     CDS             complement(4124..>6191)
                     /locus_tag="VM910_33930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HVI67545.1"
                     /translation="VLDSHGDIGASAGGPDGLFHADTRYLARLQLLFDDLQPLLLGSN
                     LRDDNSALTVDLTNPDIYHQERLVLPKDLLHVVRTIFLWRGTAYQRIGLQNHGDRRAS
                     FDLTLLFDNDFADLFEVRGEKRARRGIGSSKLQSPVDVLFDYRGLDDVTRRTALHFEP
                     RPTRLSVNAATWHLDLAPHGTASLFLAVSCNKQAETRPAKFFPGLLAHRREMREATAG
                     VTSIETSNDILNEVLCQSMADLNMLMTETPQGRYPYAGIPWYSTTFGRDGLITALQML
                     WIDPRVARGVLRRLAHYQATVTDPPSDAQPGKILHEMRGGEMAALHEVPFAQYYGSVD
                     ATPLFVLLAGLYGERTGDDEMLEELWPAIEAALRWIDGPGDPDRDGFVEYLRATEKGL
                     ANQGWKDSHDAIFHADGRLAEGLIALAEVQGYVFAAKRLAARSALRLGRREVARRLEV
                     EAHLLAERFEERFWCEELGTYAIALDGNKRPCKVRSSNAGQVLFSGMVREHRAHLVAA
                     DLLRPHFFAGWGIRTIALGEARYNPMSYHNGSIWPHDNAMIALGFARYGLKHSVEVLF
                     KGLFDAASYMDLRRLPELFCGFRREKNRGPTLYPVACAPQAWASATPFTLLEAALGLE
                     FDTERSEIRLRNPRLPAFLNEVILRGLQLGSSTVDLRISRHEDDVSMEVLRRRGKIQV
                     SIVLAH"
BASE COUNT         1117 a         2021 c         1988 g         1065 t
ORIGIN      
        1 ctggccgcgt tgcgtctgat ggcgctgata gagcgccgcc gaaatcgcgc cgaatgcata
       61 cgtgccggtg aggacgtcgg cgtgatagat gccgcaataa tcgggacgtt cgcggcccgg
      121 ctgataggcg agatgcgcca attcgtagcc ggaggctgcg tgaatgaccg gcgcgtaggc
      181 cggcagttcg gcggaggggc cagtctgccc gtagccggag atcgagcagt agattagcct
      241 cggattgagc ggggaaagcg tctcgtagtc cagccgcaag cggtgcatca cgccagggcg
      301 aaaattctcg accaggatgt ccacggtcgc gaccaggcgt cgcaccacct cgcgcccctc
      361 aggcgatttg aggtcgagca cgacgctctt cttgccgaca ttgagctggc caaacgtggt
      421 cgaacagccg ttgcgcaccg gcggacgagt ccgcatcgtc tcgccttccg cggtctcgat
      481 cttgatgacc tcggcgccca tgtcggcgag catgcgtgca caatggggac cggcgatcgt
      541 tgtcgaaaaa tccagcacgc gcaggccggc aaagcaccct gtcaaactgc tctcatcctt
      601 gtcggatatt gtcattctcg aaagctccct tcgcgacctg cgctcttggt ggtgcatcgt
      661 ccgaccctag attggggcga cgcgacaggg aagtgtttcg ccaagcgggt ttttacgggg
      721 tcgcctcgtc tcggaaattg gacccattct tgcatagcag caaagcgggc tctcgcgagg
      781 gcggcgtttt ttgaaggtct tgttcgtcac gacggagatg gaggatttcg tccgcgtagg
      841 cggactggcc gccgtttctg ctgccttgcc tcgggcgctc cggccctggg ccgatacccg
      901 tatcctgcta cccggctatg ccgacgtcgt cgaacaactc acgcatattc aaatcgttgg
      961 cgagtgtgcc gcgcgcgccg agatgccgcc ttgccggctc gggcgatctg cgacgcggga
     1021 cggcctgccg gtctacgtcc tgctgtgtcc gcaactttac gatcggccgg gcaaccccta
     1081 tggcgacgag tccggccgcg actggcccga caacgacatc cgtttcggcc gtctggcatc
     1141 ggccgccgcc gaacttgccg cgggcacttt ggacaagaat tgggcagccg atctggtcca
     1201 cgccaatgac tggcaggccg ccctggtacc ggcctatctc gcctggagcg gcgcgaaagt
     1261 cccctcgatc ctcaccattc acaacctcgc ctatcaggga cttttcccgc gggagtcgct
     1321 gcggcggatc ggtgcgccgg aaagtgcgtt tcacatcgat ggcgtcgaat tctacgacaa
     1381 gctctccttc ctcaagggcg gcatcgtcta cgcctcgcac ctgaccacgg tcagcgcgac
     1441 ctatgcgagg gagatcacca cgcccgagct cggctgcggg ctggaaggtc tgttgcgcat
     1501 gcgctcggac gcctcgcagc tcacgggcat cctcaacggc atcgatgaaa gctgggaccc
     1561 gcgattctgc gcgcagcttg cgcaaccgtt cggcgccggc gattgggagg gcaagcacgc
     1621 caacgccgag tatgtccgca agcagttcgg gcttgcggtc tcgcgcgggc cgatgttcgc
     1681 cctcgtcgcc cgcctggtgc cccagaaggg gatcgatctc gtgctctccg ctgcagacga
     1741 gattatcgat gcctgcgggc agatcgtggt cacgggctcc ggcgagccgg cgttcgagga
     1801 agcactggtt gatgcgcacc gcaggcggcc gaaggcgttc ggggtcgcga tcggcttcaa
     1861 cgacggccag gcgcggcgca ttttcgctgg gagcgatttc acgctgatgc cgtcgcgctt
     1921 cgagcagtgc gggctgagcc agatgtatgc gcagcggttc ggctcgctgc cgatcggtca
     1981 ccagaccggc gggttggctg agacaatcac cgacggcgaa accggcttcc tgttcccgca
     2041 gccttcgatc gaatccttcc tcggcggcgt gcggcgcgca ttctcgacct tcgtgtcgaa
     2101 ggaccggctc gattccatgc gccgcagcgc gatggcaaga acctttagct gggatttgtc
     2161 ggcggcctgc tacagcgcgc tctatcgcaa gactgtctcg ccctgaagcg caacggccat
     2221 cgcgcttcag gtttttgctg aagcatgacc ttgtcggaaa gctggtacgc ttaaaccacg
     2281 atcgtcgcag ggtcgctgcc gtcgctccgc cgtgtctccg gcatcaggag cgagatcacg
     2341 ccgagtccaa gcgccgccac ggcggcaagt ccgaggaaag cgacattgct gccgaaggtg
     2401 tcgctggcga aaccggcgag cacggtcgaa agcgaagcgc cgataccggt cgcggtgccg
     2461 acgatcccct gtgctaggtt gaagtgaccg ctgccgaacg cgatgtcggc gaccgtcagc
     2521 ggcacgagca cgctgaacac cgaggccgtg atgccgtcga gcaactgcac cgcgaccaga
     2581 aggtaggggt cgcggacggt cgcaaacaac accccgcgca gggcgagcgc ggcgaagccg
     2641 atcaacaaca acggccgcct gccccaggct tgcgccttgc ggccgacgct cggcgacatc
     2701 agggcgacga tcgcctgcgg cacgacgatg caaacggcaa tcagcactgg cgcccattgg
     2761 ctcgagcggg tcgtcacgat ccccgccatc agcggcaaca tggccgcatt ggaaagctgc
     2821 agcagcagta tgcagccggc aaagatcagg agcggccgcc ggcataccag agcgaccacg
     2881 ctggtcgtcc gagggtccgc ggtctcgcgc tgcaccgcgc catgcgcgcg cgcaatatcc
     2941 acttcatcct cgcgaatgcg cgccagcgac agcagggtcg ggatcgccag cgcgaaggtg
     3001 acgagaaata ccgaacggct ggagagcaga tagccgcatg tgcccatcac ggccgccgct
     3061 acgccgttgc caagcgacgc aaatcgggcg ttgcgtccca accgctcgct gatcttgagc
     3121 gggccgacca ggccgaggct gattgcggcg atcgcaggcc ccaacacgca gcttgcggcg
     3181 gcgtgcaagg tcgcggccgc caccacgacc ggaaagatcg gccaggtcgc gtaacacagc
     3241 gcggcgccgc cgatggttgc gaccgcaaga cccgcgacca gccgctccga tcgcgcggcg
     3301 tcgacgatgg cgccacccgg catctggccg atcagtgcga cgatgcttcc gatcgacagc
     3361 accaggccga tctcggcctg agtccatttc tgcgtggtca gatacactgc aatgaacgga
     3421 ccgaagccgg tctgaacgtc ggccaggaag aagatgaacc agtcgagccc gcgcaggctt
     3481 tgctgcgaag gggccggcat gctgccgccg gcttgcggca caaagtcgct gccgccggct
     3541 ggctcttcgc gccgatcgcc gtcactccgc cgccgcgaat ggattcggcc tgcaaccagg
     3601 aggcttctcc ctaattctag cgatcttcca aaggctgcag acttccgctc gcacccagca
     3661 ccacgatcga tgcgtcctcc ttgtattcgg gtgccgcggc gacctgcgcc ttggtcagtt
     3721 cgagtgtgat gctgtcgcgc ttgtcggcga tgcgcccaaa atgcagggca ttccagtcga
     3781 cgacgatctt gcggcttccg acaccgagaa agccgccgaa atcgatgacg gcggcgcgga
     3841 cggttccgct acggtccaca atgacgtcga cgatgtgtcc catgtcctcg ttggcaggac
     3901 tacggacatc gcgaccgagc acaccgtgcg cttcgcgcgg ttcgatgatg gtgacggacg
     3961 gcggtggcgg cggcgccggc tcctgcgtcg tcgcggccgg cggatttttt tgcgaatcgt
     4021 tctcggcacc ccatgccgca gcccccgcga ccgcaagaac gatggccaag atgaaccagc
     4081 ctcttacgcg catacgacct cctttttggt cgaaccgacc ttactaatgc gcgagtacga
     4141 tcgacacctg gatcttgccg cgcctgcgca gcacctccat cgagacgtcg tcctcgtggc
     4201 gactgatccg aagatcgacg gtcgaggagc ccaactgcag cccgcgcagg atcacctcgt
     4261 tgaggaatgc gggaaggcgc gggttgcgca accggatctc gctgcgctcc gtatcgaatt
     4321 cgagtcctag tgcggcttcc agcaacgtaa agggcgtcgc gctcgcccag gcttgcggcg
     4381 cgcaggccac gggatagagc gtcggtccgc gattcttctc gcggcggaaa ccgcagaaca
     4441 gctcggggag ccgccgcaaa tccatgtagg aagcggcatc gaacagaccc ttgaacaaca
     4501 cttcgaccga atgtttgagg ccgtagcgcg caaacccgag cgcgatcatc gcattgtcgt
     4561 gcggccagat cgatccattg tgataggaca tcggattgta gcgcgcttcc ccgagcgcga
     4621 tggtccggat gccccagccc gcgaagaagt gcgggcgcaa gaggtcggcg gcaaccaggt
     4681 gagcacggtg ctcgcgaacc atgccgctga acaacacctg tccggcgttc gacgagcgca
     4741 ccttgcatgg ccgcttgttg ccgtcgagcg caatcgcgta ggtgccgagt tcctcgcacc
     4801 agaacctctc ctcgaaacgt tcggcgagca gatgcgcctc gacctcgagc cggcgcgcga
     4861 cctcccgcct gccgagcctg agcgcgctgc gtgccgcgag ccgcttggcg gcgaacacgt
     4921 agccctgcac ttcggcaagc gcgatcaggc cctccgcgag acggccgtcg gcgtgaaaga
     4981 tcgcatcgtg ggaatccttc cagccctgat tggcgaggcc cttctccgtc gcgcgcagat
     5041 actcgacgaa accgtcacga tccggatcgc cggggccgtc gatccagcgc agcgccgcct
     5101 cgatcgcagg ccagagctct tccagcattt cgtcgtcgcc ggtgcgctcg ccgtaaaggc
     5161 ccgcgagcag cacgaacagc ggcgtagcgt cgacgctgcc gtaatattgc gcaaacggta
     5221 cctcgtgcag cgccgccatc tcgcccccgc gcatctcgtg caggatcttg ccgggctggg
     5281 catccgacgg cggatcggtg accgtggcct ggtagtgcgc aagacgcctg agcacgccac
     5341 gggcgacgcg cggatcaatc cacagcatct gcagtgcagt gatgagaccg tcgcgtccga
     5401 acgtggtcga ataccaggga atgccggcgt aggggtatcg tccttgcggc gtctccgtca
     5461 tcagcatgtt gaggtcggcc atcgactggc acaacacctc gttgaggatg tcgttcgagg
     5521 tttcgatgct ggtgacgccc gcggtggcct cgcgcatctc gcggcggtgt gccagcagtc
     5581 ccggaaagaa tttggccggc ctggtttcgg cctgcttgtt gcatgacacc gccaggaaca
     5641 gcgacgcggt cccatgcggc gcaagatcaa ggtgccaggt tgcggcattg acggaaagac
     5701 gcgtcgggcg cggctcgaag tgcagcgccg tgcgccgcgt cacatcgtcg agccccctgt
     5761 agtcgaacag cacgtcgacc gggctctgca gcttgctcga gccgatgccc cggcgcgcac
     5821 gcttttcccc gcgtacctcg aacaggtcgg cgaaatcatt gtcgaacaac agcgtcagat
     5881 cgaagctcgc ccggcggtcg ccatggttct gcaatccgat ccgttgatac gccgtgccgc
     5941 gccacaggaa aatcgtgcgc acgacatgca agagatcctt cggcagcacg agccgttcct
     6001 gatgatagat gtcgggattg gtgaggtcca cggtcagcgc cgaattgtca tcgcgcagat
     6061 tggagcccaa aagcagtggc tgcaggtcgt cgaacaggag ctgcaatcgc gccagatagc
     6121 gcgtatcggc atggaacagc ccgtccgggc cgccggcgga cgcgccgatg tcgccatgac
     6181 tgtcgagcac g
//
LOCUS       DATLJT010001479         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3349241,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001479 DATLJT010000000
VERSION     DATLJT010001479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349241"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1251
                     /locus_tag="VM910_33935"
     CDS             232..1251
                     /locus_tag="VM910_33935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI67546.1"
                     /translation="MIETTLRQLRYFDALAGHSHFGRAAAACGISQPALSMQIKELEE
                     ALGAVLIERGARQVRLTKFGEEAALRVRDILRSVDELGDFARASRGWLAGQLHIGMIP
                     TIAPYLLPTVIGDLTRMHPDLDIHVREALTSRLIQELVEGRLDTAIVALPVSEPSLTE
                     VALFAENFLLVRPGEDEGTPVPSSEMLREMRLLLLEEGHCFRDQALSFCNMQSSPPRE
                     ALDASSLSTLVQMVSAGMGVTLIPEMAVTVETRSASVSVARFKDPQPSRTIGMIWRKT
                     SPLAGQLLQLSEVVCSSADALREQQDGHGAISGPKRRSGRKYSLKRRAPRQKRSAVNS
                     RLARR"
     gene            1293..1667
                     /locus_tag="VM910_33940"
     CDS             1293..1667
                     /locus_tag="VM910_33940"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVI67547.1"
                     /translation="MTQERIRELTKTMLRKKLYVVLSKGGVAPEKIAEHLPRHLEYMI
                     GLEKKGVLFASGPLAEADGKTRGDGLTILRAASAQAAREIAAADPFVVHGLRSFELRE
                     WTVMEGSLGIKVNFSDQSLELA"
     gene            1700..>2377
                     /locus_tag="VM910_33945"
     CDS             1700..>2377
                     /locus_tag="VM910_33945"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI67548.1"
                     /translation="METVGLVGVGKIGLPIGENLIKSGYRVVGYRRSSLAEFEKVGGI
                     AGRSAADVGAQADVVFTCLPSSQALEDVVQGPNGLVESARRGQIIVELGSHPVPDKQR
                     HVAVLAAKGASFLDGEVSGTPGMVSARKAVIYLAGDKEACVKAERFVGGFSDSCLYFG
                     PFGAASRVKLVNNLLVAINIAGTAEAMALGLKAGVDVDLMIKAIATGSGGSTQFGIRA
                     PWMAQRRF"
BASE COUNT          430 a          707 c          776 g          464 t
ORIGIN      
        1 gaccttggag gtccagctgg ttcggccacc agtctcggtt cgtgtgtctg cgcgtctcgc
       61 ccgtgaacgg gcatttggtc acgtcatcca tcactttcct cctgtcgcgg tttgcatgat
      121 ttgtaatcgg ttccgtggct gaatttgcaa ttggttccgt ggctgcaatt ttacccgacg
      181 cattccatca ggtaaaattg actttaatga ttgctgtgat aagatattct gatgatagag
      241 acaacgctga ggcagcttcg ctatttcgat gcgctggcag gtcatagcca cttcggacgc
      301 gcggcagcag cttgtgggat ctcgcagccc gctttgtcga tgcagatcaa ggaacttgag
      361 gaggctttgg gcgccgtgct catcgagcga ggcgcacgac aagtgcggct tacgaagttc
      421 ggagaagagg ccgccctacg cgtccgcgac atcctgcgct cggtcgatga actgggagac
      481 ttcgcgcggg cgtcacgcgg ctggcttgcg ggccagttgc acatcggcat gatcccaacg
      541 attgcaccct atctgctgcc tacggtcatt ggggacctta cgcgaatgca tcccgacctt
      601 gacattcacg tacgcgaggc gctgacgtcg agactcatac aagagctcgt ggaaggccgc
      661 cttgacacgg caatcgttgc tctgccggtg tccgaacctt cgcttaccga ggtcgcgctg
      721 tttgcggaaa atttcctgct ggtccggccg ggcgaggatg agggaacgcc ggtgcccagt
      781 agcgaaatgc tgcgcgagat gaggctgctc ctgctggaag aggggcactg ttttcgcgat
      841 caggccctat cgttctgcaa tatgcaatcg tcgccgccgc gcgaggcgct ggacgcgagc
      901 tctctatcta cacttgtcca aatggtcagc gccggcatgg gtgtaacctt gatcccggaa
      961 atggccgtga cagtggagac acgctcggcg tccgtgtctg tcgcccggtt caaggatccg
     1021 caaccctcgc gaaccattgg catgatctgg cgcaagacga gccccctggc cggacagctc
     1081 ctgcagcttt ctgaagtggt ttgtagctca gccgatgcgt tgcgcgagca gcaggatggt
     1141 catggcgcca tctctggacc caaacggcgc tccgggcgaa aatattcgct caagcgccgc
     1201 gcgcctcgac agaagcgctc ggccgtgaat tcccgacttg cgaggcgata aacggcgcgc
     1261 gacaagccgc cgggacggag gggaggatac ccgtgacgca agagcgcatc cgcgagctca
     1321 ccaagaccat gcttcgtaaa aagctctacg tcgtcctctc caagggcggc gtggcgccgg
     1381 aaaagatcgc cgagcatttg ccgcgccacc tcgaatacat gatcggcctg gagaagaagg
     1441 gcgtgctgtt tgcgtccggt ccgctggcgg aggccgacgg caagacccgc ggcgatgggc
     1501 tcaccatcct acgggccgcg agcgcgcagg cggcgcgcga aatcgccgcg gccgatccgt
     1561 tcgtcgtgca cgggctgcgc agcttcgagc tgcgggaatg gacggtgatg gagggctcgc
     1621 ttggcatcaa ggtgaatttt tccgaccagt cgcttgagct ggcgtaaggt gcacgactgg
     1681 tacagtgaag ggtgagagca tggaaacggt cggcctcgtt ggcgtgggca agatcggact
     1741 gccgatcggc gaaaacctaa tcaagagcgg ctaccgagtg gtgggttacc gccgcagctc
     1801 gctcgccgag ttcgagaaag tcggcggcat tgccggccgc tcggcggccg acgtcggcgc
     1861 gcaggcggac gtcgtgttca cgtgcctgcc gtcttcgcaa gcgctcgagg acgtggtgca
     1921 aggtccgaac gggctcgtcg aatcggcgcg ccgcggacag atcatcgtcg agcttgggtc
     1981 ccatcccgtc cccgacaagc agcgtcatgt cgctgtgctc gcggccaagg gcgcgagctt
     2041 tctcgacggc gaggtgagcg gaacgccggg aatggtatcg gcgcgcaagg ccgtgatcta
     2101 tctcgccggc gacaaggagg cctgcgtcaa ggccgagcgc ttcgtcggcg gattttccga
     2161 ctcctgcctc tatttcgggc cgttcggcgc cgccagccgc gtgaagctcg tcaacaatct
     2221 tctcgtggcc atcaacattg ccggcaccgc ggaagcgatg gcgctgggct tgaaggccgg
     2281 cgtcgacgtc gatctgatga tcaaggccat cgccaccggc agcggcggct cgacccagtt
     2341 cggcatccgc gcgccttgga tggcgcagcg gcgcttt
//
LOCUS       DATLJT010001480         3922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2890919,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001480 DATLJT010000000
VERSION     DATLJT010001480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890919"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VM910_33950"
     CDS             <1..1413
                     /locus_tag="VM910_33950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVI67549.1"
                     /translation="RERLGVTRGDRVAVLALNTTDTLEVQFACGRLGAIFVPLNVRLT
                     VPELQFIVGDAAPKVMAYDADLAETALSVAKLCNVASTLLFGAGGSYETAIAAARPLG
                     AFEEVTLEDISTIMYTSGTTGQPKGATITHGMTFWNCVNLGGPAYISPSSVLLTVLPL
                     FHTGGLNCYTNPVLHAGGTVLIMRAFEPGLALKLIGDPEQGINVFFGVPAIYQFMAQH
                     PAFETADLSRLIIGGVGGAPMPVPLLNVWEARGVALQQGYGMTETSPAVLVLDREDAT
                     RKAGSAGKPVLHTEVRVVRPDGTDVAAGELGELWVKGPNITPGYWNRPDANSSSFTDG
                     WLHTGDATRVDEEGFYYIVDRWKDMYISGGENVYPAEVENVLHQITAIAEAAVIGVPD
                     VQWGEVGMAIVAMKPGQSLTETEIMAHCAKNLARFKCPRLIRFVDALPRNATGKIHKP
                     TLRKDFSVASATDLAKAKAS"
     gene            1457..2680
                     /locus_tag="VM910_33955"
     CDS             1457..2680
                     /locus_tag="VM910_33955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67550.1"
                     /translation="MKNKKLVLTAAAAAFSLLASQSAFAQKRYDTGASDTEIKIGNIM
                     PYSGPASAYGIIGKTEEAYFKMINEAGGVNGRKINFISYDDGYSPPKAVEQARKLIES
                     DEVLLIFNALGTPSQTAVQKYHNAKKVPQLFVATGASKWNDPKDFPWTMGFQPSYRVE
                     ARIFAKYILKEKPNAKVAIFYANDDFGKDYLAGIKDIFGSKASNLIVAEESYETTEPS
                     IDSHIVKLKGTGADVFVNISTPKFAAQAIKKMAELEWKPTHVLTDVSVSIGAVMKPAG
                     LEASEGILSAQYMKDASDPQWKDDEGMKKFMAFIDKYMPGANVADLNLAYGYAAAQTM
                     VQVLKQCGDDLTRENVMKQAASLKDFAPDTLIPGIKVSTSPTDFGPIEQLKMMQFKGG
                     KWELFGDIISAETGG"
     gene            complement(2786..3550)
                     /locus_tag="VM910_33960"
     CDS             complement(2786..3550)
                     /locus_tag="VM910_33960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI67551.1"
                     /translation="MSTPDKQLSAEQSRQVAETVREEIARRRISRQSLAEQAKLSLST
                     LEKVLGGRRPFTLATTVRLEQALGVSLRKGLDGPPAAQLANGGIAPDSLGAYSRRAVS
                     WLEGDYVTLRPSFGDKESIYAYRTEIKWDAAASSLVFREGERLDAAYTQWGEVAVPNQ
                     SGFIYLVTNRNGQHRLITISRPTISGEMYGIITTLLAGRGSVLTPIAAPIAFLPGKNV
                     ASPSVGRISANDPNYKLYREHLTRTTEEPFALFLKG"
BASE COUNT          805 a         1228 c         1172 g          717 t
ORIGIN      
        1 cgcgagcggc tcggcgtcac cagaggcgat cgcgtcgcag tgctggcgct gaacacgacg
       61 gatacgctgg aggtccagtt cgcatgcggc aggctgggcg cgatcttcgt gccgctgaac
      121 gttcgtctca ccgttcccga gctacagttc attgtcggcg atgccgcgcc gaaggtcatg
      181 gcctacgatg cggaccttgc cgaaacagcg ctttcggtgg cgaaactgtg caacgtcgcc
      241 tccacgcttc tttttggagc cggcggctcc tacgagaccg caatcgcagc ggcaaggccg
      301 cttggtgcgt tcgaggaggt cacgctcgag gatatctcga ccatcatgta tacctcgggc
      361 accacgggtc agcccaaggg cgcaacgatc acgcacggca tgacgttctg gaattgcgtc
      421 aatctcggcg gtcccgccta tatctcgccc tcctcggtgc tgctcaccgt gttgccgctg
      481 tttcacaccg gcggactcaa ttgttacacc aatccggtgc tgcatgccgg cggcacggtt
      541 ctgatcatgc gcgccttcga gccgggactg gctctaaaac tgatcggcga ccccgagcag
      601 ggcatcaacg tattcttcgg cgtgccagcg atctaccagt tcatggcgca gcatccggcg
      661 ttcgagaccg ccgatctcag ccggctgatc atcggcggcg tcggcggcgc gccgatgccc
      721 gtgccgctgc tcaacgtgtg ggaggcccgc ggcgtggcgc tgcagcaggg ctatggcatg
      781 accgagacgt cgcctgcggt tctcgtgctc gaccgcgagg atgccacgcg caaggcggga
      841 tcagcgggaa agcccgtgct gcacacggaa gtccgcgtcg tgcgtccgga cggcacggac
      901 gtcgcggcag gcgagctcgg cgagctctgg gtcaagggcc cgaacatcac gcccggctat
      961 tggaacaggc ccgacgccaa cagctcgtcc ttcaccgacg gctggctgca caccggcgac
     1021 gccacgcgcg tggatgagga aggtttctac tatatcgtcg accgctggaa ggacatgtac
     1081 atctccggcg gcgaaaatgt ttatccggcc gaggtcgaga acgtgctgca ccagattacc
     1141 gcgattgcgg aagccgcggt gatcggagtt cccgacgtcc aatggggcga ggtcggcatg
     1201 gcgatcgtcg cgatgaagcc gggccaatcc ctcacggaaa ccgagatcat ggcgcattgc
     1261 gcgaaaaacc tcgcgcggtt caaatgcccc cgcctgatcc gcttcgtcga tgcgttgccg
     1321 cgcaacgcca ccggcaagat ccacaaacca accctgcgaa aggatttctc cgtcgcctcg
     1381 gccaccgacc tcgccaaagc caaagcctcg taacttgcgt cactttctcc aatcccgaag
     1441 aagccaagga acccccatga agaacaagaa actcgtcctc accgctgccg cagcggcttt
     1501 ctcgctgcta gcttcccaaa gcgcgttcgc gcaaaagcga tatgatacgg gcgcttccga
     1561 caccgagatc aagatcggca acatcatgcc gtatagcggg ccggcgtccg cctacggcat
     1621 catcggcaag accgaggaag cctatttcaa gatgatcaac gaggccggcg gcgtgaatgg
     1681 ccgcaagatc aacttcatct cgtacgatga cggctatagc ccgccgaagg cggtcgagca
     1741 ggcgcgcaag ctgatcgaaa gcgacgaagt gctcctgatc ttcaacgcac ttggcacgcc
     1801 gagccagacc gcagtgcaga agtatcacaa tgccaagaag gtgccgcagc tcttcgttgc
     1861 gaccggcgcc agcaagtgga acgacccgaa ggattttccc tggaccatgg gctttcagcc
     1921 cagctatcgc gtcgaggcgc ggatctttgc gaaatacatc ctgaaggaaa agccgaacgc
     1981 caaggttgcg atcttctacg ccaacgacga tttcggcaaa gactatctcg cgggcatcaa
     2041 ggatatcttc ggcagcaagg catcgaacct gatcgtggcc gaagagagct atgagaccac
     2101 cgagccctcg atcgattccc acatcgtcaa gctcaagggc accggcgcgg acgtgttcgt
     2161 caatatctcg acgccgaagt tcgcagcaca ggcgatcaag aaaatggcgg agctcgaatg
     2221 gaagccgacg cacgtgttga ccgacgtgtc ggtctcgatc ggggcggtca tgaagccggc
     2281 cggcctcgag gcttccgagg gcatcctgtc tgcccagtat atgaaggatg cttccgatcc
     2341 gcagtggaag gacgatgaag gcatgaagaa attcatggcc ttcatcgaca aatacatgcc
     2401 gggcgccaac gtcgccgatc tcaatctcgc ctacggctat gccgccgcgc agaccatggt
     2461 gcaagttctg aagcagtgcg gcgacgatct cacgcgtgaa aacgtcatga agcaggccgc
     2521 gagcttgaag gattttgcgc cggacacctt gatccccggc atcaaggtct cgaccagccc
     2581 gaccgacttt gggcccatcg agcagctcaa gatgatgcag ttcaaaggcg gcaagtggga
     2641 gcttttcggc gacatcatca gcgccgaaac cggcggctag ctacgttgcc gttccggatc
     2701 gcgcgaaccg gaatggcaat ctacgatctc gagattcgcc atcggcgcat ccgggtcggc
     2761 ttagccgagt tggatgcgcc gtcgtctatc ccttcaagaa cagcgcgaac ggctcttcgg
     2821 tcgtgcgcgt gaggtgttcg cgataaagct tgtagttcgg atcgttggcg gaaatccgcc
     2881 ccacgctggg gcttgcgaca ttcttgcccg gcaggaacgc aatgggagcg gcgatcggcg
     2941 tcaacaccga gccgcgcccc gccaatagcg tcgtgatgat gccgtacatt tcgccggaga
     3001 tggtcggccg cgatatggtg atcagccggt gttgcccgtt gcggttggtc acgagataga
     3061 tgaatccgga ttggttcggg accgccactt cgccccattg ggtgtaggcg gcatccagcc
     3121 tttcaccttc gcggaagacc agcgacgacg ctgccgcgtc ccattttatc tccgtgcgat
     3181 aggcatagat cgattccttg tcgccaaagg acggacgtaa cgtcacataa tcaccctcca
     3241 gccaggacac cgcgcggcgc gaataggcgc cgaggctgtc gggcgcgatg ccgccattgg
     3301 cgagttgcgc cgcgggcgga ccatccaatc ccttgcgcaa ggagacgcca agcgcctgct
     3361 ccagccgcac cgtcgttgca agcgtgaacg gacgccggcc gcccagcact ttctcgaggg
     3421 tcgaaaggct gagcttcgcc tgctcggcga gcgactgccg ggagatgcgg cgacgcgcga
     3481 tctcctcgcg cacggtttcc gccacctgac ggctttgctc ggcggaaagc tgcttgtccg
     3541 gcgtcgacat gattaaccct gctccaatcc ggccattcta gcgtcccgac tgcgaagttc
     3601 gagttcgcga caataggatg tggatttctg aaccgccaca ctagcagacg cacaatccag
     3661 cacaaaaccg cacaaaaccc cacaccactc agccgcctcg ggacggagcg aggccaaaca
     3721 ttgccaatca ttctgctcgc gccacggacg gcagttcgca aaatccgcaa acgcgaacca
     3781 tcgctttggg agaagccaaa atgtccacct gcgagatcga ggccagcagt tcatcattct
     3841 ggtcgcggtt atttactctg ctgctgtttc ttctgctgca aggcgtcgtc gtgctgtggg
     3901 tcgccttcgt ggcattgatg gt
//
LOCUS       DATLJT010001481         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3811477,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001481 DATLJT010000000
VERSION     DATLJT010001481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3811477"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VM910_33965"
     CDS             complement(<1..1242)
                     /locus_tag="VM910_33965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVI67552.1"
                     /translation="MANATTATPAMMPAQAAPSERMEPRRVVAFIIMVFGMFMSILDI
                     QVVSASLTDIQAGLSASSTEVSWVQTAYLIAEVIAIPLSGFLSRALGTRLLFAISAAG
                     FTVASLLCGFASSSAEMILWRAIQGFLGAGMIPTVFASAYTIFPRSKQYIIAPIIGLV
                     ATLAPTVGPTVGGFITDAMSWHWLFFINVPAGIIITIGVLALVDFDQPNFALLERFDW
                     WGLIFMAGFLGALEYVLEEGPQYEWLQDTSVAVCAAISFVSAIAFFWRVLTAGEPIVD
                     LRAFTNRNFAVGCVLQFCIGIGLYGLTYVYPRYLAEVRGYSALMIGQTMFVSGITMFL
                     MAPIVGRLLQRYDLRYIIAFGLITFAMGSYQMTWITRDFDFYELLVPQILRGIGMMCA
                     MVPTNNIALGTLPPDRVKNASG"
     gene            complement(1287..2612)
                     /locus_tag="VM910_33970"
     CDS             complement(1287..2612)
                     /locus_tag="VM910_33970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVI67553.1"
                     /translation="MAAARDQAARFLRSEPGQGEAKADREASAAIAEQLRSHLAEESR
                     RRPAETPSSPATDDPAIEPARPADPVMAVTATARKSGKRRKVLMGVVALLALAGGGYG
                     VYYTLVGRFYVSTDDAYVRANNTILGARVSGHISAIFPGDNTLVRKGDVIFRIDDGDY
                     KIAVEAARTRIATQEATIERIGRQVAAQVSAVEQAQANLASAEAGLKRAGLDYERQQT
                     LSTKGFASHATFEQSEAARDQGVAGVKAAQAAYDNARDMVAVTKAQQVESQAQLAELK
                     TQLARAERDLEFTYVRAPVDGTFGNRMINVGDYISVGQRLGNVVPLDDVFIDANFKET
                     QLKRIRPGQPVTIKVDAYGFRKFTGTVDSISPAAGSVFTLLPPDNATGNFTKIVQRLP
                     VRIRVPKDVARQNLLRAGMSVYATVDTNKDADDADSTADLDSPLMTHPE"
BASE COUNT          555 a          908 c          925 g          483 t
ORIGIN      
        1 gccggaggcg ttcttcacgc ggtcaggcgg cagcgtgccg agcgcgatgt tgttggtcgg
       61 caccatcgcg cacatcatgc cgatgccgcg caggatctgc ggcaccagca gctcgtagaa
      121 atcgaagtcg cgcgtgatcc atgtcatctg ataggagccc atcgcgaagg tgatgaggcc
      181 gaaggcgatg atgtagcgca ggtcgtaccg ctgcagcagg cggccgacga tcggcgccat
      241 caggaacatc gttatgcccg agacgaacat ggtctggccg atcatcagcg cgctgtagcc
      301 ccgcacttcc gcgagataac gcggatagac gtaagtgagg ccatacagcc cgatgccgat
      361 gcagaactgc aagacgcagc cgacggcgaa attgcggttg gtgaaggcgc gcaaatcgac
      421 gatcggctcg cctgcggtca ggacgcgcca gaaaaaggcg atcgcggaaa cgaagctgat
      481 ggccgcgcag accgccaccg aggtgtcctg cagccactcg tattgcgggc cttcctcgag
      541 cacatattcg agcgcgccga gaaaacccgc catgaagatc aggccccacc agtcgaaccg
      601 ttcgagcagc gcaaaattcg gctgatcgaa atcgaccagc gccagcacgc cgatggtaat
      661 gatgatgccg gccggcacgt tgatgaagaa cagccagtgc cacgacatcg catccgtgat
      721 gaagccgccg accgtcggac ccacggttgg cgccagcgtt gcgaccagtc cgatgatcgg
      781 cgcgatgata tattgcttgg agcggggaaa tatcgtgtag gcagaggcga acacggttgg
      841 gatcatgccc gcgcccagaa agccctggat cgcgcgccac aagatcatct ccgcgctcga
      901 agacgcgaag ccgcacaaca ggctcgcgac ggtgaagccc gcggccgaaa tcgcaaacaa
      961 cagccgcgtg ccgagcgcgc gcgacaggaa tccggacaac ggaatcgcga tcacttccgc
     1021 gatcagatag gcagtctgta cccaggacac ctcggtcgaa cttgccgaga gccccgcctg
     1081 gatatcggtc agcgatgccg agacgacctg gatgtccagg atcgacatga acatcccgaa
     1141 caccatgatg atgaaggcaa cgacgcgccg cggctccatg cgctcggagg gggcggcctg
     1201 cgctggcatc atggccggcg ttgcggtggt ggcgttcgcc ataactcaaa ttctcgtcaa
     1261 acggcgcgcc gccgggcgca ccgagtttat tccggatggg tcatgagcgg cgagtcgaga
     1321 tcggcggtcg agtcggcatc gtcggcatcc ttgttggtgt cgacggtggc atagaccgac
     1381 atgccggcgc gcagcaggtt ctgccgggcg acatccttcg gcacgcggat gcgaaccggg
     1441 agccgctgca cgatcttggt gaaattgccg gtcgcgttgt cgggcgggag cagcgtgaac
     1501 accgagcccg ccgcgggcga aatgctgtcg acggttccgg tgaatttgcg gaagccgtag
     1561 gcatccacct tgatcgtcac cggctggccc ggacgaatgc gcttgagctg cgtctccttg
     1621 aaattggcgt cgatgaagac gtcgtcgagc ggcacgacat tgccgagccg ctggccgacc
     1681 gagatataat cgccgacatt gatcatgcgg ttgccgaacg tgccgtccac gggcgcgcgc
     1741 acataggtga attcgaggtc gcgctcggcc ctggcaagct gggtcttgag ctccgcaagc
     1801 tgcgcctgcg attccacctg ctgcgccttc gtcacagcga ccatgtcgcg cgcattgtca
     1861 taggccgcct gcgccgcctt gacgccggca acgccctggt cgcgggcggc ttccgactgc
     1921 tcgaacgtcg cgtgcgaggc aaagcccttg gtgctcaggg tctgctggcg ctcgtaatcg
     1981 aggccggcgc gcttcaatcc ggcttcggcg gaggcaagat tggcctgcgc ctgctcgacg
     2041 gcggagacct gcgcggcaac ctgacggccg atccgttcga tggtcgcttc ctgcgttgcg
     2101 atcctggtgc gcgcggcctc gaccgcgatt ttgtagtcgc cgtcgtcgat ccggaagatc
     2161 acgtccccct tgcgcaccaa agtgttgtcg ccggggaaga tcgccgagat atggcccgac
     2221 acgcgcgcgc cgagaatcgt gttgttggcg cggacatagg catcgtcggt ggaaacatag
     2281 aagcgtccga ccagcgtgta atagacgccg tagccgccac ctgccagcgc gagcagcgcg
     2341 acaacgccca tcagcacctt tctgcgcttg ccggatttcc gcgcggtggc cgtcactgcc
     2401 atcacgggat ccgcgggccg ggcgggttcg atcgccgggt cgtcggtggc aggcgaggat
     2461 ggcgtctccg cgggccgacg cctggattct tcggcgaggt gcgagcgaag ctgctcggca
     2521 atcgcagccg aagcctcgcg gtccgccttg gcctcgccct gccccggttc ggaccgaaga
     2581 aagcgcgcgg cctggtctct cgctgcggcc atatggcctc cccacttcaa tcgcgtcgcg
     2641 acacgcggca gccgaccggc cccacggcct actagcccaa aaatatcatt gaccgaacgg
     2701 ttcggtcaag ctatgtaaca acccgccgag cctagactgc ggacctttcg agacggttcc
     2761 gttgtccaaa tccttccaga gacacttaat caatggttgc agccgagccc gcgatccttc
     2821 agcccgccgc agacgaggtc aattcgaagc gccgccagat tctcgatggt g
//
LOCUS       DATLJT010001482         9202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2432041,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001482 DATLJT010000000
VERSION     DATLJT010001482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432041"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VM910_33975"
     CDS             <1..173
                     /locus_tag="VM910_33975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67554.1"
                     /translation="ADEPTGNLDEDTADDVLALARDLVARSGCGFLMVTHSARLAATL
                     DRRVTLHAGLIS"
     gene            170..2632
                     /locus_tag="VM910_33980"
     CDS             170..2632
                     /locus_tag="VM910_33980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67555.1"
                     /translation="MRRAIWTLAVLLSHWRRHPLQLATLLIGLIAATALWSGVQALNQ
                     QARNAYDRAAAVFGGTRTAMLVGSNSATVPQALFAKLRRAGWPVSPVLEGRVQIGGHA
                     YRLLGIEPVTLPSQVGNAPILGRSEFMSFITPPGRTLVSPETLSDLDLAEGASPATSG
                     GATLPPLVAQPQLVPGVLVVDIAVAQKLLDKRDQLSRLLVGTFKGTAEPLERVAGDQL
                     RLVQPDAETDLERLTDSFHLNLTAFGLLSFFVGLFIVNSAIGLAFEQRLPVLLTLRAC
                     GISARLLNVTLVVELVALAIVAGAVGMICGYLIAATLLPDVAASLRGLYGAPVGGELN
                     LRPEWWLAGLVISITGALAAAASGLVKAIRLPVLSVAQPQAWLQAQHRWLIVQSACAM
                     IAFAAAGSLVWFGNSLIAGFAVLAALLLGAALILPALLEALLQLGERTSDGALSVWFW
                     ADGRQQLSGLSLALMALLLALAVNVGVATMVETFSRTFLTWLDGRLAADVYLSAKDDA
                     QATEIVGWLAGHPEVEAIMRGGRAETQTDGMPIEILGLPDHATYREHWPLLQVADDAW
                     RMLAAGDDCFVSEQLSRRNNLAVGDQLDLPTPVGNWPLRVAGIYADYGNPKGQVAVNA
                     AALFRHFPDTPRTRLALRVTPAGIPALITQLSDKFGLNDRNLLDQTTLKAESTRIFNR
                     TFAVTAALNTFTLLVAGIALLTSLLTLANSRLPQLAPLWAVGLTRRNLAAIELTRTMS
                     VALITALLALPLGLLVAWCLIAIVNVKAFGWRLPFHVFPLQLAKLLGVAFVTSFLASL
                     LPVLRLARMQPAELVKIFANER"
     gene            2622..3710
                     /locus_tag="VM910_33985"
     CDS             2622..3710
                     /locus_tag="VM910_33985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVI67556.1"
                     /translation="MSADPRISRRAFASGLAAIGLAPRCALAQGFAGLGERADDYAQV
                     TPGKIFSFPADHGPHLDFRIEWWYVTANLVDGDGAAYGAQWTLFRQALTPGERSEGWD
                     NQQIWMAHVAVTRADTHRFAETYARGGVGQANVSANPFEAFIDSWAMRGEDGFDANTV
                     SPLELTAQAKDFSYLLRLSADRPLILQGDRGYSKKSEREQASYYYSQPFFKARGQLAI
                     DDRSHEVTGLAWMDREWSSQPLASDQTGWDWLALHCNSGEKLMLYRMREKSGPNYISG
                     NWILPDGTARQLAPGDIDMTPGATSDIAGHTLPLQWQVKIPSLSLSIASTALNPKAWM
                     GTSFAYWEGPISFHGSHTGVGYLELTGY"
     gene            complement(3800..4987)
                     /locus_tag="VM910_33990"
     CDS             complement(3800..4987)
                     /locus_tag="VM910_33990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67557.1"
                     /translation="MKSGLLVAVAAGAVLLAAPASAQVKIGILNDQSGVYADYGGKYS
                     LEAARMAVEDFGGEVLGKKIEIVTADHQNKPDLASAIARRWYDAEGVDMITELTTSSV
                     ALAVQELSKEKKKIDIVVGAATSRITGDACTPYSFHWAYDTRALAVGTGGALVESGGD
                     TWFFLTADYAFGYALEKDTSDIVTAKGGKVLGSVRVPLNSSDFSSFLLQAQSSKAKIV
                     GLANAGLDTTNSIKQAAEFGIVKGGQKLAGLLMTISEVNGLGLEAAQGLILTEGFYWD
                     HDDKTRAFSERFIKRTGHMPSMIHAGTYSATLSYLKAVKAAGTKDSEAVAKKLKELPV
                     DDAFAQGKVLENGRMVHDLYLFEVKKPSESKKPWDYYKQIAVVPGDKAFFTAKESGCP
                     LTK"
     gene            complement(5085..8261)
                     /locus_tag="VM910_33995"
     CDS             complement(5085..8261)
                     /locus_tag="VM910_33995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVI67558.1"
                     /translation="MNLGRLSVNQPILAMVLSIVLLIVGALAYTTLPVSEYPEVVPPT
                     VVVTAQYPGASAQTISDTVAAPIEQQINGVEDMLYMYSQATSNGQLTITITFKLGTDL
                     DKAQVLVQNRVAIATPQLPDEVQRNGVVTRKNSPDILMVVFMLSPDDSLDQLYISNYA
                     LLQVRDQLLRLDGIGDIQIFGARDYSMRLWLDPDKIATLGLTASDVIAAIRAQNLQIT
                     GGQLASPPISDRAFQPNLTFTGRLKDVAQFEDIVVKAGEEGRTVRLRDVARVELGALD
                     YSTNSFELRKTAVAMLVTQRPGSNALATAKGISTTMARLKAGFPKGLDYDIGYNPTEF
                     IRQSISELIKTIYEAMALVVIVVLVFLQGWRPAIIPIAAIPVSLVGTFAAMAALGYAI
                     NNLTLFGLVLAVGIVVDDAIVVVENVERHLEHGMSRRDAALKTMEEVGGALVSIALVL
                     CAVFVPTAFLGGITGQFFQQFAVTIAVATAISCFCSLTLSPALASQILVPHQEKRPPA
                     AWNLIARAWEAFVGVFNRGFDWASRSYADVANFVIQHRAVMLVIYVVLICSAGWLIAT
                     TPQGYIPAQDRGYVIISAQLPGAASLARTTEIVRRIESIALDTPGISHIAVFAGLSGA
                     TRTQASNSAALFPVFDEPEVRLKKGLNAQVITNELRKRLAAIEGAFIIVIPPPPIPGI
                     GTGGGFTMRIQDRQGRGPELLAAATDELVAAARKAPGLTSVFSPFSANTPQVFVDIDR
                     IKAQKLGVPIQNVTDAIETYFGSTYVNDFNILGRTYHVTAQADLRFRKETADLARLRT
                     RNANGDMVMLGSVVDFRDISGPDRVARYNLYPAAELQGEGLPGTSSATAIATMKRLAD
                     ETLPSGFSYEWTDLSYQQVTGGNTGLFVFPICVLFVYLVLAAQYGSWSLPFAVILIVP
                     MCLLAATVGVRIMGQDVNILTQIGFVVLVGLAAKNAILIVEFARDIELEGRERLEAVI
                     EACRLRLRPILMTSFAFILGVLPLVVSSGSGSEMRQAVGVAVFFGMIGVTLFGLVFTP
                     IFYMVVRNLADRRAAKKPVPSRGEMAAK"
     gene            complement(8258..>9202)
                     /locus_tag="VM910_34000"
     CDS             complement(8258..>9202)
                     /locus_tag="VM910_34000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI67559.1"
                     /translation="FVTNVEFKDGDMVHAGDLLYLIDSRPFEAVAAQADGQLADARAK
                     VELAKRELDRGLNLVQTSAVSEQVVDQRRQALQAAHAAEIVAEGALKAAQLNVEFTHV
                     PAPISGRVSRHLVSVGNLVSGSDTGTSTLLTTIVSLDPIYIYFDVDEATYLKNNKLWF
                     EGKRPSSRDTPNPVQVALTGEIKPSHEGRMDFIDNRLDVSTATLRSRAVIPNHDLSIL
                     PGQFGRVRLIGSSPYEALLIPDTAVATDQSRKIVFVIKDDDTVEARPVVLGPLDEGLR
                     VVREGLQAEDKVIIDGIQRARVGAKVNPRAGTIGGSKS"
BASE COUNT         1589 a         2954 c         2953 g         1706 t
ORIGIN      
        1 tcgccgacga gccaacagga aatctcgacg aagacaccgc cgatgacgtg ctggcgctgg
       61 cgcgcgacct ggttgcgcga agcggttgcg gctttctgat ggtgacccac agcgcgcggc
      121 ttgccgcaac gctggaccgg cgggtcaccc tgcatgcggg actcatctcg tgagacgcgc
      181 gatctggacc ctggcggtcc tgctcagcca ttggcggcgg caccccctgc agcttgcaac
      241 gctcctgatc gggttgatcg cggcgaccgc gttgtggagc ggcgtgcagg cgctgaacca
      301 gcaggcgcgc aacgcctacg atcgcgcagc cgccgtgttc ggcggcacgc gaacggcgat
      361 gctggtcggc agcaacagtg cgacggtgcc gcaagcactg tttgcaaaac tgcgccgcgc
      421 aggctggccg gtgtcgccgg tcctggaggg ccgcgtgcag atcggcgggc acgcctatcg
      481 gctgctcggc atcgagccgg tcaccctgcc ttctcaagtc ggcaatgcgc cgatactcgg
      541 acgatccgag ttcatgtcct tcatcacgcc gccggggcgc acgctggtct cgccggaaac
      601 gctgtccgac ctcgatctcg cagaaggcgc ttcgccggcg acgagcggcg gcgccacgct
      661 gccgccgctc gtggcacagc cgcagctcgt tcccggcgtg ctcgtggtag acatcgccgt
      721 cgcgcaaaaa ctcctcgaca aaagggatca gctttctcgc ctgctggtcg gcaccttcaa
      781 gggcacggcc gaaccgctcg aacgcgtcgc aggcgatcaa ttacgtctgg ttcagccgga
      841 cgccgaaacc gatctcgagc ggctgaccga cagctttcat ctcaacctga cggcattcgg
      901 cctgttgtcg ttctttgtcg gtctcttcat cgtcaactcg gcgatcgggc ttgccttcga
      961 gcagcgcctc ccggttttgc tgacgcttcg cgcctgcggc atctctgcaa gactcttgaa
     1021 tgtcacactt gtggtcgagc tggttgcgct ggcgatcgtc gcaggcgcag tcggcatgat
     1081 ctgcggctat ctgatcgcgg ccacgctgtt gcccgacgtt gcggcatcgc ttcgcggcct
     1141 ctacggcgcg cctgtcggcg gcgagctcaa tctcaggccc gaatggtggc tcgcgggtct
     1201 tgtgatctcc atcaccggtg cgctcgccgc ggccgcgagc ggcctggtca aggcgatccg
     1261 cttgccggtt ttgtcggtcg cgcagccgca ggcctggcta caagcgcagc accgctggct
     1321 gattgtccag agcgcctgcg caatgatcgc ctttgccgcc gccggatcgc tggtgtggtt
     1381 cggcaattcc ctgattgcgg gctttgccgt gctggcggca ctgctgctcg gcgcggcact
     1441 gatcctgccc gcgcttctcg aagcactgct gcagcttggc gagcgcacgt cggacggcgc
     1501 gctcagcgtg tggttctggg ccgatggccg ccagcaactg tccggcctgt cgctggcgct
     1561 gatggcgctg ctgcttgcgc tcgcggtcaa tgtcggcgtc gcaaccatgg tggagacgtt
     1621 cagccggacg ttcctgacct ggctcgacgg acggctcgcg gccgatgtct acctctcggc
     1681 caaggacgat gcgcaggcaa ccgagattgt gggctggctc gccgggcatc ccgaggtcga
     1741 agcgatcatg cggggtggcc gggcggaaac gcaaacagac ggcatgccga ttgaaattct
     1801 ggggctgccg gatcacgcga cctatcgcga gcactggccg ctgttgcagg ttgcggacga
     1861 tgcctggcgc atgcttgccg ccggcgacga ctgcttcgtc agcgagcaac tttcgcgccg
     1921 caacaacctt gcagtcggcg atcaactgga tttgccgacg cccgttggca actggccgtt
     1981 gcgggttgcc ggcatctatg ccgactacgg taacccaaag ggacaggtcg ccgtcaatgc
     2041 cgccgccttg ttcaggcatt ttcccgacac gccgcgcacg cggcttgcgc ttcgcgttac
     2101 gcccgcgggc atccccgcgc tgatcactca gctttcggac aaattcggcc tcaatgatcg
     2161 caatctgctg gaccaaacga cgctgaaggc ggaatcgacg cgcatcttca accgcacctt
     2221 tgcggtaacc gcggcgttga ataccttcac gcttctggtt gcgggaatag cgctcctgac
     2281 cagcctgttg acgctcgcca attcgcgcct gccgcaactt gccccgctct gggcggtcgg
     2341 gctgacgcgg cgcaatctcg ccgcgatcga gctcacgcgg accatgtcgg ttgcgctgat
     2401 caccgcgctg ctggcgctgc cgctcgggct cttggtcgca tggtgcctga tcgccattgt
     2461 aaatgtgaag gcattcggct ggcggctgcc gtttcacgtc tttcccctgc agctcgcaaa
     2521 gctgctcggc gtcgccttcg tcacctcatt cctcgcctcg ctcctgccgg tgttgcggct
     2581 cgcgcgcatg cagccggccg agctcgtcaa gatttttgcc aatgagcgct gatcccagga
     2641 tctcccgccg cgcttttgca agcggtctcg ctgcgatcgg cctagctccg cgatgcgcgc
     2701 tggcgcaggg cttcgccggg ctcggcgaac gggcggacga ttacgcccag gtcactcccg
     2761 gaaaaatctt ctcgtttccg gcagatcacg ggccccacct cgattttcgc atcgaatggt
     2821 ggtacgtgac cgcgaacctc gtcgatggcg atggcgcagc ctatggcgcg caatggacgc
     2881 tgttccggca ggccctgacg cctggcgagc ggagcgaagg gtgggataac cagcagatct
     2941 ggatggcgca cgtggcagtc acgcgcgccg acacgcaccg ctttgccgag acttacgcac
     3001 gtggcggggt cggacaggcg aacgtcagcg caaatccgtt cgaggccttt atcgattcat
     3061 gggcgatgcg cggcgaggac ggatttgacg ccaatactgt ctccccactc gaactcaccg
     3121 cacaggcaaa ggacttcagc tatttgctgc gcctttcggc cgaccggccg ctgattcttc
     3181 agggcgatcg tggctacagc aaaaaatccg agcgcgagca ggcctcttat tactacagtc
     3241 aaccgttctt caaggcgcga ggacagctcg cgatcgatga tcgctcgcac gaggtcaccg
     3301 ggctcgcctg gatggatcgg gagtggagca gtcagccgct cgcgtcagat caaaccggat
     3361 gggactggct cgcgctgcac tgcaattcgg gcgagaagct gatgctgtac cggatgcgcg
     3421 agaaaagcgg ccccaactat atctccggca actggatact gcccgacggg acggcgcgac
     3481 agcttgcacc gggcgatatc gacatgacgc ccggcgcaac aagcgacatc gcaggacaca
     3541 cgcttccgct gcaatggcag gtgaagatcc cgtccctgtc gctatctatc gcatccacgg
     3601 cgctcaatcc gaaggcgtgg atggggacaa gcttcgccta ttgggaaggc ccgatcagct
     3661 ttcacggcag tcacacgggc gtcggatatc ttgaactgac gggatattga ttcgccgcgc
     3721 gccggcgcgg cgacattgtt cggttcgccc ggcgcaacgc tgcgcgctac ccggagatcg
     3781 cttggccgcg acgacgacgc tacttcgtca gcgggcagcc gctttctttc gccgtgaaga
     3841 acgccttgtc gccgggaacg acggcgatct gcttgtaata gtcccacggc ttcttcgatt
     3901 cggagggctt cttgacctcg aacaaataaa gatcgtggac catgcggccg ttctccagca
     3961 ccttgccttg cgcgaaggca tcgtcgaccg gcagctcctt cagcttcttg gccaccgcct
     4021 cgctgtcctt cgtgccggca gccttcaccg ccttcagata gctcagcgtc gccgaatagg
     4081 taccggcatg gatcatgctc ggcatgtggc cggtgcgctt gatgaagcgt tcggagaagg
     4141 cgcgcgtctt gtcgtcatga tcccagtaaa agccttcggt gaggatcagg ccctgggctg
     4201 cctccaggcc caggccgttt acctcggaaa tcgtcatcag caggcctgcg agcttctgac
     4261 cgcccttgac gatgccgaac tcggccgcct gcttgatcga gttggtggtg tcgagaccgg
     4321 cgttggcgag gccgacgatc ttggccttcg agctctgtgc ctgcaacagg aaggacgaga
     4381 agtccgacga gttcaggggc acccgcaccg aacccagcac cttgccgcct ttggcggtga
     4441 cgatgtcgct ggtgtctttt tccagcgcat agccgaaggc gtagtccgcc gtcaggaaga
     4501 accaggtgtc gccgccggac tcgacgagcg cgccgccggt tccgaccgcg agcgcccgcg
     4561 tatcgtaggc ccagtggaaa ctgtagggcg tgcaggcatc gccggtgatg cgcgaggtcg
     4621 cggcgcccac cacaatgtcg atcttcttct tttccttgga tagctcctgc accgcgagcg
     4681 ccaccgagga cgtcgtgagt tcggtgatca tgtcgacgcc ctcggcatca taccagcgtc
     4741 gcgcaatcgc gctcgcaaga tcaggcttgt tctggtgatc ggcggtgacg atctcgatct
     4801 tcttgcccag cacttcgccg ccgaaatcct cgaccgccat gcgggctgct tccagcgaat
     4861 atttgccgcc ataatcggcg tagacgccgg actgatcgtt caggatgccg atcttgactt
     4921 gtgccgaggc gggcgccgcg agcaacactg cgcctgccgc caccgccacc aacaatcccg
     4981 atttcatgct ttttgctccc agcaagtttt tgattccgcg cggatgttat tgaataaccc
     5041 tgcgcctgcc catctatttc gcttaggcca aaagtcggaa tggctcactt ggcggccatt
     5101 tccccgcgcg agggcacggg cttcttggcc gcgcggcggt cggccagatt gcgcaccacc
     5161 atgtagaaga tcggcgtgaa gaccagcccg aacagcgtga cgccgatcat gccgaagaac
     5221 acggcgacgc cgaccgcctg ccgcatttcc gagccggagc cggaggacac caccagcggc
     5281 agcacgccga gaatgaaggc gaacgaggtc atcaggatcg gcctcaggcg caaccggcag
     5341 gcctcgatca cggcctcgag ccgctcgcgg ccctcgagct cgatgtcgcg cgcgaattcg
     5401 acgatcagaa tggcgttctt cgccgcgagc ccaaccagca ccacgaagcc gatctgggtc
     5461 agtatgttga cgtcctgccc catgatgcgc acgccgacgg tggcggcgag caggcacatc
     5521 ggcacgatca ggatgacggc aaacggcagg ctccaactgc catattgcgc ggccagcacg
     5581 aggtagacga acagcacgca gatcggaaac acgaacaggc cggtattgcc gccggtgacc
     5641 tgctgatagg agaggtcggt ccattcatag gagaagccgc ttggcagcgt ctcgtcggca
     5701 agccgcttca tcgtcgcgat cgcggtcgcc gagctcgttc ccggcagccc ctcgccctgc
     5761 agttcggccg cggggtagag attgtagcgc gccacgcgat ccggccccga gatgtcgcgg
     5821 aagtccacga cgctgccgag catcaccatg tcgccattgg cgttgcgcgt gcgcagccgc
     5881 gcaaggtcgg cggtttcctt gcggaagcgc aggtcggcct gcgccgtgac gtgataggtg
     5941 cggccgagga tgttgaagtc gttgacatag gtcgagccga aataggtctc gatggcgtcg
     6001 gtgacgttct ggatcggcac gcccagtttc tgtgccttga tgcggtcgat atcgacgaac
     6061 acttgcggcg tattggcgct gaatggcgag aacaccgagg tgagcccggg cgctttccgc
     6121 gccgccgcaa ccagctcgtc cgtcgccgcc gcgagcagct ccggcccgcg cccctgacgg
     6181 tcctggatcc gcatggtgaa gccgccgccg gtgccgatgc ccgggatcgg cggcggcgga
     6241 atgacgatga taaatgcgcc ttcaatggca gcgagccgct tgcgcaactc gttcgtgatc
     6301 acctgtgcgt tcagcccctt cttcaaccgc acttccggct catcgaacac agggaaaagc
     6361 gccgccgagt tgctggcctg ggtgcgggtc gctcccgaca ggcccgcaaa gaccgcgatg
     6421 tgggagatgc cgggcgtatc gagcgcgatg ctttcaatcc tgcgaacgat ctcggtggtc
     6481 cgcgccagcg aggccgcgcc cggcaattgc gcggatatga tcacgtagcc gcgatcctgc
     6541 gccggaatat agccctgcgg cgtggtggca atcagccatc cggcactgca gatcagcacg
     6601 acatagatca cgagcatcac tgcgcggtgc tggatcacga aattggcgac atcggcatag
     6661 gaacgggacg cccagtcgaa gccgcggttg aagacgccga cgaaggcctc ccaggcgcgg
     6721 gctatcagat tccatgccgc gggcggccgt ttttcctgat gcggaacgag gatctgggaa
     6781 gccagcgccg gcgacagcgt cagcgagcag aagcaggaga ttgcggttgc gactgcgatg
     6841 gtgaccgcga actgctgaaa gaactgcccg gtgatgccgc cgagaaacgc ggtcggcacg
     6901 aacaccgcac acaagaccag cgcgatcgag accagcgcgc cgccgacctc ctccatcgtc
     6961 ttcagcgcgg catcccgcct gctcatgcca tgttcgagat gccgctcgac attttcgacg
     7021 acgacgatgg cgtcgtcgac gacgatgccg accgcgagca cgagaccgaa cagcgtgaga
     7081 ttgttgatgg catagccgag cgccgccatc gccgcaaacg tgccgaccag cgacaccggg
     7141 atcgccgcga tcgggatgat cgccgggcgc cagccctgca ggaacaccag caccacgatg
     7201 acgacgaggg ccatcgcttc atagatggtc ttgatcagct cgctgatcga ctgccggatg
     7261 aactcggtcg gattgtagcc gatgtcgtag tcaagcccct tcggaaagcc ggccttcagc
     7321 ctggccatgg tggtggagat gcccttcgcg gtcgcaagcg cattcgatcc gggacgctgg
     7381 gtcaccagca tcgcgaccgc ggtcttccgc agctcgaagc tgttggtgga atagtcgagc
     7441 gcaccgagct cgacccgcgc cacgtcgcgc agccgcaccg tgcggccctc ttcgccggcc
     7501 ttgacgacga tgtcctcgaa ctgggcgacg tccttcaggc ggccggtgaa cgtcaggttg
     7561 ggctggaagg cgcggtcgga gatcggcggg cttgcaagct ggccgccggt gatctgcagg
     7621 ttttgcgccc ggatcgcggc gatgacgtcg ctcgccgtca ggccgagcgt cgcaatcttg
     7681 tcgggatcga gccataaccg catcgagtag tcgcgcgcgc caaaaatctg gatatcgccg
     7741 ataccgtcca gccgcagcag ttggtcgcgc acctgcaaca gcgcgtagtt ggagatatag
     7801 agctggtcga gcgagtcgtc cggcgacagc atgaacacga ccatcagaat gtcgggcgag
     7861 ttcttgcgcg tgacgacgcc gttgcgttgc acttcgtcgg gcagttgcgg ggtcgcgatc
     7921 gcgacgcggt tctggaccag cacctgcgcc ttgtcgagat cggtgccgag cttgaaggtg
     7981 atcgtgatcg tgagctggcc gttcgaggtt gcctggctgt acatgtacag catatcctcg
     8041 acaccgttga tttgctgttc gatgggagcc gcaaccgtat cggaaatggt ctgcgccgag
     8101 gcgcccggat attgcgccgt gaccacgacc gtcggcggca ccacttccgg atattccgag
     8161 accggcaggg tggtgtaggc gagcgcgccc acgatcagca gcacgatgga caggaccatt
     8221 gccaggatgg gctgattgac ggaaagccgg ccgagattca tgacttgctg ccgccgatcg
     8281 tgcccgcgcg cgggttcacc ttggcaccga cgcgggcgcg ctgaatgccg tcgatgatca
     8341 ccttgtcttc ggcctgcagg ccctcgcgga ccacgcgcag cccctcgtcg agcggaccga
     8401 gcacgaccgg cctcgcttcg accgtgtcgt catccttgat cacgaacacg atcttgcgcg
     8461 actgatcggt cgcgaccgca gtatccggga tcaacaacgc ctcgtagggc gagctgccga
     8521 tcagcctgac gcggccgaac tggcccggca gaatcgaaag gtcgtggttc ggaataacgg
     8581 cgcggctgcg cagcgttgcc gtggacacgt cgagccggtt gtcgatgaaa tccatgcgtc
     8641 cctcatgcga gggctttatc tcgccggtca gcgcgacctg cacgggattg ggcgtatcgc
     8701 gcgagcttgg acgcttgccc tcgaaccaga gcttgttgtt cttcaggtaa gtcgcctcgt
     8761 ccacgtcgaa atagatgtag atcggatcga gcgacacgat ggtggtgagc agtgttgacg
     8821 tgccggtgtc gctgccggac acgagattgc cgacgctgac gagatggcgg ctgacgcggc
     8881 cgctgatcgg cgccggcaca tgggtgaact cgacattgag ctgggccgcc ttcagcgcgc
     8941 cttcggccac gatctcggcg gcgtgcgcgg cctgcagcgc ctggcggcgc tgatcgacga
     9001 cctgttcgga gactgcgctg gtctggacca ggttcaggcc gcggtcgagc tcgcgcttgg
     9061 caagctccac cttggcacgc gcgtccgcaa gctggccgtc ggcttgcgcg gcgactgctt
     9121 cgaacgggcg ggagtcgatc aggtaaagca ggtcgccggc gtgaaccatg tcgccgtcct
     9181 tgaattcgac gttggtgacg aa
//
LOCUS       DATLJT010001483         2673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4041520,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001483 DATLJT010000000
VERSION     DATLJT010001483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041520"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..118
                     /locus_tag="VM910_34005"
     CDS             <1..118
                     /locus_tag="VM910_34005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI67560.1"
                     /translation="IFKSYDDLVDHCCAAWNKLVDQPWRIMSIGLRQWAHGF"
     gene            complement(416..685)
                     /locus_tag="VM910_34010"
     CDS             complement(416..685)
                     /locus_tag="VM910_34010"
                     /inference="COORDINATES: protein motif:HMM:NF041373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HVI67561.1"
                     /translation="MKGNHKRNTGPMLSSLRCGAKTRSGKPCKSPAAHGKKRCRMHGG
                     ASGSGAPRGNKNALKHGRYTRDAIAERAHIRELVRQARKLILQIE"
     gene            complement(682..1464)
                     /locus_tag="VM910_34015"
     CDS             complement(682..1464)
                     /locus_tag="VM910_34015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67562.1"
                     /translation="MPGRETKQHNKEEKTVHKVTPAERIAVNKHLARREAKSQVRLKI
                     SKNRSDPQIELDHPDKRVGQALIMDVFASADGDFVNGIMSQLADASLHGQDISDHGLN
                     FMLSVIKGIEPRDQLEAMLAAQMAAVHVASMTFARRLAHVEEIPQQDSVERAFNKLTR
                     TFAMQMEALKRYRSGAEQKVTLQHVSVAEGGQAIVGNVTQVPRENRQEQPAQEQPAQE
                     KAAREKGATPPPPLPDTNVVPMTIMGKSKQHDPVAVRRKSNK"
     gene            complement(1831..>2673)
                     /locus_tag="VM910_34020"
     CDS             complement(1831..>2673)
                     /locus_tag="VM910_34020"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI67563.1"
                     /translation="STEAAGEVRMSQDLVLYETDAKVGFVTLNRPEKLNALSVDLRRE
                     LAAALTRADEDPATSVVVLRGAGRSFCVGYDLASGSGSEAWRHDALKYHERLGTSLAL
                     ELMPWYMRKPVLASVQGHALGAGCELAMFCDLTIAADDAMFGEPEVRYSQAGPGFVMP
                     WIIGFKKARELLYLGDMIDAPTALELGMVNRIVPRDDLSKATLKFARRMTLIASEALA
                     TTKLAINRGADAAGFRNAMQAGIDVAAPLYAAQTEVATQFRELARKEGLGAALKWRKA
                     QFET"
BASE COUNT          453 a          842 c          779 g          599 t
ORIGIN      
        1 catcttcaaa tcctacgacg atctcgtcga ccattgttgt gcggcatgga acaagctcgt
       61 cgatcagcct tggcgcatca tgtccatcgg actgcgccaa tgggcccacg ggttctgatc
      121 aatgggattt ggtattagcc gttggatgcc gccgcaggat aactccactg tcgatatcga
      181 cgatcatcgc cacgccggcg tgggtccctc tgcagaaccc tacgttatgg cggcagtgcg
      241 tgctgacggt gctgacgcaa aagacctgaa cccatcaggc ctttgcgtgc caacccgcag
      301 cacagctgga tcgcccggta gatccgaccc cacgttcgtt cgaaatcccc gtgctttggt
      361 ctcggatgcc ggattgcggg gttcccgatc actgcccgct ggtcagcgac gcaggttact
      421 ctatttggag gatcagcttg cgcgcctgcc gcaccagttc tcgaatgtgc gcgcgttccg
      481 caatagcgtc gcgagtatag cggccatgct taagcgcgtt cttgttgcct cgcggggcgc
      541 ctgatcccga cgcgccccca tgcatgcggc agcgcttctt gccatgggcc gccggtgact
      601 tgcagggctt gcctgaacga gtcttagcgc cgcagcggag actggaaagc atcggccccg
      661 tgttgcgctt gtgattgccc ttcatttgtt tgacttgcgc cgcacagcaa cagggtcgtg
      721 ctgcttgctc ttgcccatga ttgtcatcgg cacgacgttc gtatcgggaa ggggcggcgg
      781 gggcgtcgca cccttctccc gcgcagcctt ctcctgcgca ggctgctcct gtgcaggctg
      841 ctcctgccgg ttctcgcgtg gcacctgtgt gacgttgccg acaatggcct ggccgccttc
      901 ggcgactgaa acatgttgta gtgtgacctt ctgctcggcg ccgctccggt aacgtttcag
      961 tgcctccatc tgcatcgcaa acgtgcgggt gagcttattg aaagcgcgct ccacactgtc
     1021 ctgctgagga atctcttcca catgggcgag gcggcgcgcg aacgtcatgc tcgccacgtg
     1081 cacggccgcc atctgggccg caagcattgc ctcgagttga tccctcggct caataccttt
     1141 gatgactgac agcatgaagt tgagcccgtg atcgctgatg tcctgaccgt gcaaactagc
     1201 gtcggcgagt tggctcataa tgccgtttac aaagtccccg tcagccgacg cgaagacgtc
     1261 catgataagc gcctggccga ctcgcttgtc cggatggtcc agctcaattt gcgggtcgct
     1321 ccgattcttg gagatcttga gtctgacctg cgactttgct tcacgtctgg caagatgctt
     1381 gtttactgca atgcgctccg ccggcgtcac cttatgaact gtcttctctt ctttattgtg
     1441 ctgtttcgtc tctcttcctg gcatctgtgc gtctccaagc gaccccgcgt cgcaatacgt
     1501 gaggcgatcg tgcttccgaa agtgtgcctc ttaaaatcgg tctgaaggca tcccgcatcc
     1561 tagggtgatt cggaggatca ataatcctca ctaatttcgg ccaaggaact actttgctag
     1621 gggttgtttt tcaaaatttt ttattccgtg ggagttcgaa acatagcgcc ggtcttgggg
     1681 gctgaccgtt tcaaaatcga tggggcagcg agatgccttg atccgagcgt tttttctacc
     1741 aatggccgcc gcgcctacgc aaagtccctc gcgccggttc aagctttcat tgctccttca
     1801 tgcacgccgg cgccaatcag tcctttgcga tcaggtttcg aactgagcct tgcgccattt
     1861 caacgcggcg cccaatcctt ccttgcgggc gagctcgcgg aactgggttg cgacctcggt
     1921 ctgcgcggca tagagcggcg cggccacgtc gatgccggcc tgcatcgcgt tgcgaaatcc
     1981 ggcggcgtcg gcaccgcgat tgatggcgag cttggtggtg gcgagcgctt cggaggcgat
     2041 caacgtcatg cgccgggcaa acttcagcgt ggccttggat agatcgtcac gcggcacgat
     2101 gcggttgacc atgccgagct cgagcgccgt cggcgcgtcg atcatgtcgc cgagatagag
     2161 aagctcgcgc gccttcttga agccgatgat ccacggcatc acgaagccag gcccggcctg
     2221 cgagtatctc acttccggct cgccgaacat cgcatcgtcc gcagcgatcg tgagatcgca
     2281 gaacatcgcg agctcgcagc cggctccgag cgcgtgcccc tgcaccgagg caagcacagg
     2341 cttgcgcatg taccagggca tcaactcgag agcgaggctg gtgccgagcc gctcgtgata
     2401 tttgagcgca tcgtgtcgcc aggcctcgct gccggaaccg ctggcgaggt cgtagcccac
     2461 gcaaaagctg cggccggcgc cgcgcagcac caccacgctg gtcgccggat cctcgtccgc
     2521 cctggtcagc gccgcggcaa gctcgcggcg cagatccacg ctcagcgcat tgagcttttc
     2581 cgggcgattc aacgtgacga aaccaacctt cgcgtcggtc tcgtagagca ccaggtcctg
     2641 gctcattctt acttcccccg ccgcctccgt gct
//
LOCUS       DATLJT010001484         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3812213,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001484 DATLJT010000000
VERSION     DATLJT010001484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3812213"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VM910_34025"
     CDS             <1..910
                     /locus_tag="VM910_34025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI67564.1"
                     /translation="QIGIFSIAALGLNILVGFTGQISIGHAAFFLFGAFSSAYINNNF
                     PIPVFFAIPLAGVVTALVGLIFGVPAARLKGLYLVIATLAAQYILLDFFSRAEWFSGG
                     SVPASANPFSIFGYTLRGDRQYFYVVLAYLLVSFILVTNLMRTRDGRALVAIRDHYLS
                     AEIMGINLTKYRTLSFALAAFFAGIAGALYAHYQLVVSQEGFGIERSVLFLAMIIIGG
                     TGSIMGTLMGTAFVVLVPESMEWLSNQLKGSAFDHALGLNNNLTFLREITIGVIIIGF
                     LMFEPDGLAHRWRQIKAYWKLYPFSH"
     gene            964..2244
                     /locus_tag="VM910_34030"
     CDS             964..2244
                     /locus_tag="VM910_34030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67565.1"
                     /translation="MKLTSLLSTASLALMIGTASAGAQQIAIGHLEDLSGGTADVGTP
                     YGQAVVDTFAWVNKNGGVGGKQLSVDSNDYGYKVPQAIALYKKWSAPDNKVAAIMGWG
                     TADTEALTGFLAEDKIPDMSGSYAAALTDPEGTSGKAKPAPYNFFYGPTYSDAMRAML
                     TWAAEDWKAKGKPGKPKFVHMGANHPYPNAPKAAGEALAAELGFEVLPPLVFALTPGD
                     YSAQCLSLKSSGANYAYLGNTAASNISVLKACKTAGVDVQFLGNVWGMDENAMKTAGD
                     AADGVIFPLRTAVAWGGNAPGMKTVMEISKMSDPTGKVYRPVHYIAAVCSAMYMKEAL
                     DWAAAHGGATGENVAKGFYQKKDWVPAGMEGVCNPSTWTEKDHRGSMKVDIYRAKVAG
                     ATDGDINDLMAKGTIKLDKVKTVELPRKAEWFGW"
BASE COUNT          414 a          723 c          684 g          425 t
ORIGIN      
        1 ccagatcggc atcttttcga tcgcggccct cggcctcaac atactggtcg gcttcaccgg
       61 gcagatctcg atcgggcatg ccgcgttctt cctgttcggc gcgttttcgt ccgcctacat
      121 caacaacaat ttcccgatcc ccgtgttctt cgccattccg ctcgccggcg tcgtcaccgc
      181 gctggttggc ctgatcttcg gcgtgccggc ggcgcggctc aagggccttt acctcgtgat
      241 cgcgacgctt gccgcgcaat atatcctgct cgattttttc tcccgcgccg aatggttttc
      301 cggcggctcg gtgccggcca gcgccaaccc gttctcgatc ttcggctaca cgctgcgcgg
      361 cgaccggcaa tatttctatg tcgtgctcgc ttatctgctt gtcagcttca tcctggtcac
      421 caacctgatg cgaacccgcg acggccgcgc gctggtcgcg atccgcgatc actacctctc
      481 cgcggaaatc atgggtatca atctcaccaa ataccgcacg ttgtcgttcg cgcttgccgc
      541 tttcttcgcc ggcatcgccg gcgcgctcta tgcgcactat cagctcgtgg tctcgcagga
      601 gggattcggc atagagcgct cggtgctgtt tctggcgatg atcatcatcg gcggcaccgg
      661 atcgatcatg ggcacgctga tgggaaccgc tttcgtcgtg ctggtaccgg aatcgatgga
      721 gtggctcagc aaccagttga aaggcagcgc cttcgatcat gcgctcgggc tgaacaacaa
      781 ccttaccttc ctgcgcgaga tcacgatcgg cgtgatcatc atcggctttc tgatgttcga
      841 gcccgacggg ctcgcgcatc gctggcggca gatcaaggcc tactggaagc tctatccgtt
      901 ttcgcattga ggttggaaaa cgaccggcta caattgctat caacgaacaa taggaggaaa
      961 accatgaagc taacgtctct tttgagcacg gcgtcgctgg cgctcatgat cggcaccgcg
     1021 tcggcgggcg cgcagcagat cgcgatcggc catctcgagg acctctcggg cggcacggcc
     1081 gacgtcggca cgccctacgg ccaggccgtt gtcgatacct tcgcctgggt caacaagaac
     1141 ggcggcgtcg gcggcaagca gctctcggtc gacagcaacg actacggcta caaggtgccg
     1201 caggcgatcg cgctctacaa gaaatggtcc gcgccggaca acaaggtcgc ggcgatcatg
     1261 ggatggggta ccgccgatac cgaggcgctg accggcttcc tcgcggagga caagataccg
     1321 gacatgtcgg gctcctatgc cgcggcgctg accgatcccg agggcaccag cggcaaggcc
     1381 aagcccgcgc cctacaattt cttctacggt ccgacctatt cggacgcgat gcgcgcgatg
     1441 ctgacctggg ccgccgagga ctggaaagcc aagggcaagc ccggcaagcc gaaattcgtg
     1501 cacatgggcg ccaaccatcc ctatcccaac gcgccgaagg ccgcaggcga ggcgctcgcc
     1561 gccgagctcg gcttcgaggt gctgccgccg ctcgtgttcg cgcttacgcc cggcgactac
     1621 agcgcacagt gcctctcgct gaagagctca ggtgccaatt acgcttatct cggcaacacc
     1681 gcggcctcca acatctccgt gctgaaggcc tgcaagaccg ccggcgtcga cgtgcagttc
     1741 ctgggcaatg tctggggcat ggacgagaac gcgatgaaga ccgcgggcga tgctgccgac
     1801 ggcgtgatct tcccgcttcg taccgccgtc gcctggggcg gcaatgcgcc cgggatgaag
     1861 acggtgatgg aaatttcgaa aatgtccgat cccaccggca aggtctatcg ccccgtgcac
     1921 tacatcgcgg ccgtctgctc ggcgatgtac atgaaggaag cgctcgactg ggcagccgcg
     1981 catggcggtg ccaccggcga gaacgtcgcc aagggcttct accagaaaaa ggattgggtg
     2041 ccggccggca tggagggcgt ctgcaacccc tcgacctgga cggagaagga ccatcgcggc
     2101 tcgatgaagg tcgacatcta tcgcgcgaag gtcgccggcg cgaccgatgg cgacatcaac
     2161 gacctgatgg ccaagggcac gatcaagctc gataaggtca agaccgtcga gctgccgcgc
     2221 aaggcggaat ggttcggctg gtgagt
//
LOCUS       DATLJT010001485         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1973589,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001485 DATLJT010000000
VERSION     DATLJT010001485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973589"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VM910_34035"
     CDS             complement(<1..880)
                     /locus_tag="VM910_34035"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HVI67566.1"
                     /translation="MRDRLRLMLGVAVVLTMALSAERAPAEDPNAAPNPYRAVESWAK
                     LPEGRAWGQAIGVDVDRDGTSVWVFDRCGAKTCEGSNIAPIQKFDASGKLVTSFGAGM
                     INWPHGLFADRDGNVWVTDGRGGNGKGHTVIKFSSDGKVLMTLGKPGVAGDGPDTFNG
                     PSDVLVAPNGDIFVADGHGEKTNARIVKFSKDGRFIKAWGKAGKGQGEFDVPHALAMD
                     SSGRLFVADRSNDRIQIFDQDGKFLAEWRQFGRPSGLYIDRNDILYAADSQSGEKFNM
                     SFRQGIRIGSVKDGKVT"
     gene            complement(943..1791)
                     /locus_tag="VM910_34040"
     CDS             complement(943..1791)
                     /locus_tag="VM910_34040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67567.1"
                     /translation="MPAERRLIVDAQVHLWKAESPDWQWVPGLKPQLPEPFTIERLVP
                     MMDEADVDRAVIVPPSWPGDRNDYALEAVKRYPERFRVMGRIPLQDPKSADLLPKWKQ
                     QPGMLGVRVIFNNPRTIPWLTDGTADWFWPAAEKAGLPVMCFAPGRTAAFGQIAQKHP
                     QLTLIIDHMGVNMAMADDGRLEGAIGETVALAKYPNVSVKVSASPGISREPYPFRDVT
                     VHLKRVFDAYGPQRSYWGTDLTNSFAKASYRQRITQFTQELSFLSESDKDWVMGRAIM
                     ERLKWT"
     gene            2399..4480
                     /locus_tag="VM910_34045"
     CDS             2399..4480
                     /locus_tag="VM910_34045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67568.1"
                     /translation="MACAALLAVTPPRPDLVLMTARICLKLRRPRRACDAVAGMNAID
                     ARQVADPDRTAGEITNIAAALRTRGLRAEAKDVLRSAIALAPRAATPRSALILLLEED
                     LRAAGGNISLEQWAELENLLRKSVIESPHLAAPRLALAACLRQEARYRDAQTAPARVV
                     APRLRAEAENLLREAVAEEPRQAAPHLALAEFLLENLRYAEAETVAVAAVQRCPASAE
                     LRLVLARIQHQLLRREDAKNTIEALIAIAPENAWAWFEYGRILWNSFEPADGAFERAA
                     DVSGNDIALLAGVARHFLYDLDYEKAAKYYERLLDLHPSMWDNFVICGQYATCLKKIA
                     RAQEAAQIIATALDRCRLAAERAKGEGLELIKREEALLPLQAGRADKSFATLRSMQDI
                     AAPTPRYDRVEYLPRTPERLQRLAAIVDSRDVFVLLQGPSFATFAARLHEFAGFEFAI
                     ATLNSFPPIERELRRINRCADILLFTQPGSIRSWHPELMEFLARSSPNLVVANRYALS
                     GLSEFGVSEREFVARHDKRLLLVHSDGPPLPSRPLHFDNGPSVSWLIPLLLFARPRRI
                     FLFGADGGSNPSFSKRPYFYYDDYDADVEPREFLNRPGMISFKELPRKLDEYNRRQHI
                     NAVNGDRVIDFAFRSLEANFGIQVPPIFNVCPHSTHRIFPRIDIDDALAKLADDRARS
                     APERRAKRASN"
     gene            complement(4613..>5159)
                     /locus_tag="VM910_34050"
     CDS             complement(4613..>5159)
                     /locus_tag="VM910_34050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVI67569.1"
                     /translation="LWGGKDWPPVAYSPQTRLLYIPANDNLCTELTGEAPKYVQGERF
                     MGVAKNVMKMVADADHVGELQAWDLDAGKKVWTTNLPSFNWGPVLATGGGLLFAGGTN
                     DRMFRAFDAKTGKILWEFPTGSGVAAVPVSFEVDGKQYIAVQSGWGVDPAKMQARMNL
                     IFPGKYPDVPQGGAIWVFAVK"
BASE COUNT          922 a         1668 c         1550 g         1019 t
ORIGIN      
        1 ccgtcacctt gccgtctttg acgctgccga tccggatgcc ctgccggaac gacatattga
       61 atttctcgcc cgactgggaa tcggccgcgt agagaatgtc gttgcggtcg atgtagaggc
      121 cgctcggccg gccgaactgc cgccattcgg cgagaaattt tccatcttgg tcgaagatct
      181 gaatgcggtc gttcgagcgg tcagcgacga acagtcgccc gctcgagtcc atggcgagag
      241 catggggcac gtcgaattcg ccttgtccct tgcccgcttt gccccaggcc ttgatgaatc
      301 ttccgtcctt cgagaacttc acgatgcggg cgttcgtctt ctctccgtgt ccgtccgcga
      361 cgaagatgtc gccattgggc gcgaccagga catcggacgg cccattgaag gtgtcgggcc
      421 cgtcgcccgc gaccccgggc ttgcccagcg tcatcagcac ctttccatcg gaactgaact
      481 tgatgaccgt gtggcccttg ccgttgccgc ccctgccgtc ggtcacccag acgttgccgt
      541 cccggtccgc gaacaggccg tgcggccaat tgatcatgcc ggcgccgaag ctcgtcacca
      601 acttgccgga ggcgtcgaac ttctgaatcg gcgcgatatt cgagccctcg caggtctttg
      661 cgccgcaccg atcaaagacc cagacgctgg tgccatctcg atcgacgtcg actccgatcg
      721 cctgccccca cgcgcggcct tccggaagct tcgcccaact ctctaccgcg cgatagggat
      781 tgggcgcggc attgggatcc tcggcgggcg ctcgctccgc cgacaaggcc attgtcaaaa
      841 caacggcgac gccgagcatc aatctcagac gatcacgcat gtcacgtcct ccctttgcag
      901 attgtacttg cgctgttctc ggacagcttg aaccgacgtt cctcacgtcc acttcaggcg
      961 ctccatgatc gcgcggccca tcacccagtc cttgtcgctc tcgctgagga agctcagctc
     1021 ttgcgtgaac tgggtgatgc gctggcgata gctcgccttg gcgaacgaat tggtgagatc
     1081 ggtgccccaa tagctgcgtt gcgggccgta ggcgtcgaac acgcgcttca aatgcaccgt
     1141 gacgtcgcgg aacggatatg gctcgcgcga gataccgggc gaggccgaca ccttgacgct
     1201 gacgttgggg tattttgcga gcgcgacggt ctccccgatc gctccctcga gccttccgtc
     1261 gtcggccatg gccatgttca cgcccatatg gtcgatgatc aacgtgagct gcggatgctt
     1321 ctgggcaatc tggccgaacg ccgcggtgcg gccgggcgcg aaacacatga cgggaagtcc
     1381 ggccttttca gccgccggcc aaaaccagtc cgcggtgccg tccgtgagcc aagggatcgt
     1441 gcgcgggttg ttgaagatca cgcgcacgcc caacatcccc ggctgctgct tccatttcgg
     1501 taggagatcc gccgatttgg gatcctgcag cgggatgcgg cccatcacgc ggaaacgctc
     1561 gggataacgc ttgaccgcct cgagcgcata gtcgttacgg tcgcctggcc acgacggcgg
     1621 cacgatcacc gcgcgatcga cgtccgcttc gtccatcatc ggcaccagcc gctcgatggt
     1681 gaacggctcc ggcaattggg gcttgagccc gggaacccat tgccagtccg gcgactccgc
     1741 cttccacaga tggacctgcg catcgacgat caggcgtcgc tcggccggca tggattgatc
     1801 ctccctcgtt cgcggcggcg gcgttcagcg ccggcgccgt gcacaaccga ttgtgcgacg
     1861 ttccgacccc cggccacaac gggattgaac gcaagggcag ccttgaccgg cataggcggc
     1921 cgcgccttgc ggcgcctcga atctgcgagc gcacgagcaa caggcgcggg tcacctggtt
     1981 gtcgccggcg aagcacagct gcgccgaatt tcgacgagtc tcgccgctgc tgttctcact
     2041 tcgatttatg gagaattaac tcgccgtgcc cttgttatag gcaaacgttt ttcatcgctg
     2101 gtattcgcct gcctcgcgtc gcctcgcaaa ccgcgcgcca ttgcatcaac tcgcgcgtcg
     2161 ttctgacgaa gagggaagtc gcattgtttt tgcgacgctg cagcccagcg atttacacgc
     2221 cgtttttatc cgccgccggt ttggaaagtt aatgctgcgg ccatgttctg gaaagcctca
     2281 aagagagagc gtcacgaatc ggacgacgag cattttgggg tggggccgta tcgtgcgctt
     2341 cggggcaggt cgcatcgttt ggcaaagttg tcgcgcagcg gccggcgcgg cgatcaatat
     2401 ggcctgtgcg gcgttgctgg ccgtcacgcc gcctcgcccc gacctggtgc tgatgacagc
     2461 ccgtatctgc ctgaagctga ggcgtcctcg gcgagcctgc gacgccgtcg cagggatgaa
     2521 cgcgatcgac gctcggcaag ttgccgaccc tgatcgcacg gccggagaga tcacgaacat
     2581 agcggcggcg ctccgcacgc gcggcctgcg agcggaggcg aaggatgtgc tgcgcagcgc
     2641 cattgctctg gcgccgcgcg ccgccactcc ccggtccgca ctgatcttgt tgttggagga
     2701 ggatcttcgc gccgccggcg gcaacatatc gttggagcaa tgggcggaac tcgagaacct
     2761 tttgcgaaaa tcggtgatcg agtcgccaca cctcgccgct ccccggcttg cactcgccgc
     2821 ctgtctgcga caggaggctc gataccggga cgcgcagacg gcgccagcga gggtcgtcgc
     2881 accgagactg cgcgcggagg ccgaaaatct gctgcgggag gcggtggcgg aagaaccgcg
     2941 ccaggccgct ccccacctcg cgcttgccga gttcttgctc gagaaccttc gatatgcgga
     3001 ggctgaaacc gttgctgttg cggcggtgca gcgttgcccg gctagcgcag aactgcgctt
     3061 ggttcttgcg agaattcagc accaactcct acgtcgcgag gatgccaaaa acacaatcga
     3121 agcgctcatt gccatcgctc ctgaaaatgc atgggcctgg ttcgaatacg gcaggatttt
     3181 gtggaattcc ttcgagccgg ccgacggcgc cttcgagcgc gccgcggatg tgtcgggaaa
     3241 cgatatcgct ctgctggcag gggtcgctcg acattttcta tacgatctcg attacgagaa
     3301 agccgcgaag tattacgaac gacttctcga tcttcatccg agcatgtggg acaattttgt
     3361 gatctgcgga caatacgcaa cctgcttgaa gaagattgcc cgcgcacagg aggccgccca
     3421 aatcatcgca acggccctcg accgctgtcg actcgcggct gaacgcgcaa aaggagaggg
     3481 tctggagctt atcaagcgcg aggaggcact cctgcctctc caggccgggc gcgcggataa
     3541 gtcctttgcg acattgcgat cgatgcagga catcgccgcc ccgactccac gctacgatcg
     3601 ggtcgagtat ctgccccgaa ctccggagcg gctgcagcgg ctggcggcaa tcgtggactc
     3661 gcgggacgtt ttcgtgttgt tgcagggccc atcattcgcg acgttcgcgg cacgcctgca
     3721 tgaatttgcc ggcttcgaat tcgccattgc cactctcaat tcttttccgc cgatcgaacg
     3781 ggaactgcga cgcatcaatc gttgtgcgga tatattgctt ttcactcagc ctgggtccat
     3841 tcgctcctgg catcccgaac tcatggaatt tctcgcccgc tcgtctccaa acctggtcgt
     3901 ggcgaaccgt tacgcgctct ccgggctctc cgagttcggg gtaagcgaga gggaatttgt
     3961 cgcgcggcac gacaaacgac tgcttctcgt acattccgat ggcccgccgc tcccgtcccg
     4021 gccgctgcac ttcgacaacg gacccagcgt ctcgtggctc atcccgcttt tgctgttcgc
     4081 ccggccccgg cggattttcc tgtttggggc cgatggcggt tcgaacccaa gcttcagcaa
     4141 acgtccctat ttctactatg acgactacga cgccgacgtc gaaccgcggg aattcctcaa
     4201 cagaccgggc atgatctcgt tcaaggagct gccgcgcaag ctcgacgagt acaatcgccg
     4261 ccagcatatc aatgccgtca atggcgacag agtcatcgac ttcgcttttc gtagcctcga
     4321 agccaacttc gggattcaag tcccgcccat attcaatgtg tgtccacaca gcacccatcg
     4381 aatctttccg cgcatcgaca tcgacgacgc gcttgcaaag ctggcggacg atcgcgcgcg
     4441 ctccgccccc gagcggcggg cgaagcgcgc atcaaattga ggcccacccg cggacgcctg
     4501 gcgcaacgtt gtccgtttcg gagatcgacc ggatgctttg cgaataccgg gaataaggta
     4561 cggggcccgg acgacgacgc gccggagccc cgatcgatct tcagccaagc ctttacttca
     4621 cggcgaagac ccagatcgcg ccgccttggg gcacgtccgg atatttgccg gggaagatca
     4681 gattcatgcg agcctgcatt ttcgcgggat cgacacccca gcccgactgg accgcgatat
     4741 attgcttgcc gtcgacctca aacgataccg gcaccgcggc cacgcccgac ccggtcggga
     4801 attcccacag gatcttgccg gtcttggcat cgaaggcgcg gaacatgcga tcgttggtgc
     4861 cgcccgcgaa caggaggccg ccgccggtgg cgaggacggg gccccagttg aacgacggca
     4921 agttggtggt ccacaccttt tttccagcgt cgagatccca cgcctgcagc tcgccaacgt
     4981 ggtcggcgtc ggcaaccatc ttcatcacgt tctttgcgac acccatgaag cgctcgcctt
     5041 gcacatattt gggagcttca ccggtcagtt cggtgcacag gttgtcgttg gcgggaatgt
     5101 agagcaggcg cgtctgcggg ctgtaggcga ccggcggcca atctttgccg ccccacagg
//
LOCUS       DATLJT010001486         3728 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3122814,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001486 DATLJT010000000
VERSION     DATLJT010001486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122814"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VM910_34055"
     CDS             complement(<1..693)
                     /locus_tag="VM910_34055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67570.1"
                     /translation="MQIIVRPARAEELQAAEELVVRSINDLTVRHGFGAMASLRPADF
                     QQFSLNDDPDGLWVAEADGELAGFAFSWISGNFWFLAELFVSPDHQGRGIGDTLLKRT
                     FEHAAKADADNKALITFTFNTVSQGLYIRRGLYPRLPVYFFKAEARSSRQRSAEILDH
                     AEIGNTDRDRARLAEFDLNALGFARDKHHGFLSGNAGITGVFLYAGTDCVGYAYIDSG
                     GHIGPLAVAEPGL"
     gene            complement(794..2932)
                     /locus_tag="VM910_34060"
     CDS             complement(794..2932)
                     /locus_tag="VM910_34060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVI67571.1"
                     /translation="MAVRWGHGKIPGFLRRGWGVRGSLFAAFAAIAGMAIVISAGAGF
                     VLRHLGDTMVDLSGQDIPRLAASLQLSTQAATLAAQGPAVLASQNEDALNQRIKKMKE
                     IQEVTQEKLGEIIELGVDKRVISALSETIKNIQEAAQSLISAARERVTLAAEHDNQYD
                     ALRSAQAAFVAAASPAMLDAQTRITAILGSASLSAADAIEAAQTVDQLGNVVASGNLA
                     AADLIAALSAGSSDKLDDIAREYKSAQGRVRSNLDLLPSNRGTNALREAAAKLLALGE
                     GKGGVFSLRQKELDAEDYGQTVLEETRKLNVGLDVSVQQLVDDVQKGTAASTFMTRQQ
                     ISFATTIMLVMGAATLIGSVLFVWRYVGGNILRRIGSLQRSMQLLSDGDLESEIYQSH
                     QKDEVASMANALQVFRESMIEARALSAEQDRDRAAKIERTSRIEARIAEFEATVRCAL
                     DNLQDAANSMQSTAQSMSTTADQSNALVSTVVSAAEETSANVQTVSVGTEELSSSITE
                     IGRQVVSSSEIARKAVAEASATDATMQGLADNASRISVVVDLIQVIASQTNLLALNAT
                     IEAARAGEAGRGFAVVASEVKSLANQTAKATEEIRLQIANMQAVTTSAVNAIRNIGQT
                     IGEINDVTTAIAAAVEEQGAATREIARNIQHAAGGTSQVSSNIVGVSTASADAGVAAN
                     EVLKASAALRREADGLRSEIDAFLNNIRAA"
     gene            complement(3092..>3728)
                     /locus_tag="VM910_34065"
     CDS             complement(3092..>3728)
                     /locus_tag="VM910_34065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI67572.1"
                     /translation="YNRTPAYVAPVYNWTGFYIGAHVGGAFSSDNNFSGLAIGNNSNG
                     RVLGGLQAGYDWQFNPNWVVGVEGQYSWLSGGVAAVFPGGFIYNNNQRGLGSLTGRVG
                     YTWGPGMVYAKGGYAYSDNNESVTLGGIPVAFATAGDHTNGYTIGAGVEYVFAPNWSV
                     KAEYQYYNFGDTHFVAPAALFATGNFTTNDNVFRAGVNYRINWGGPLVGRY"
BASE COUNT          624 a         1169 c         1218 g          717 t
ORIGIN      
        1 caggccgggc tccgctaccg ccagcggacc gatgtggccg ccggaatcga tgtaggcata
       61 accgacgcaa tcggttccgg cgtagagaaa gacgcctgtg atgccggcat ttcccgacag
      121 gaatccatga tgcttgtccc gtgcaaaccc gagcgcgttc aggtcgaact ccgcaagcct
      181 tgcccgatcc cgatcggtgt ttccgatctc ggcatgatcc aggatctcgg cgctgcgttg
      241 gcggcttgac cgcgcctcgg ccttgaagaa gtagaccgga agccgggggt agaggccgcg
      301 ccggatgtag agtccctgcg aaacggtgtt gaacgtgaaa gtgatgagcg ccttgttgtc
      361 ggcgtcggcc ttggcggcgt gctcaaaggt tcgtttcaaa agcgtatcgc cgatcccgcg
      421 accttgatgg tcgggagaga cgaacaattc tgcgaggaac cagaagttgc cgctgatcca
      481 gctaaacgca aagcccgcta attcgccgtc ggcctcggcg acccacaggc catccggatc
      541 gtcgttcagg gagaattgtt gaaagtccgc cggtcgcagg ctcgccatcg cgccaaagcc
      601 gtggcggacg gtgagatcgt tgatgctgcg cacgacgagc tcttccgccg cctgcagttc
      661 ctcggcgcgc gcgggccgga cgatgatttg catggcggga gcttatcgtc aaagcccgcc
      721 gaaacaagtt gccgcgcttt gtcctgccct cgatgcggct gcgtcaaacc ggcgcgcttg
      781 tcgcggtccg gtctcaggca gcgcggatgt tgttcaggaa tgcgtcgatt tcggaccgca
      841 gcccatcggc ctcgcggcga agcgcggcgg atgccttcaa gacctcgttc gccgcaacgc
      901 ctgcatctgc cgaagcggtc gagacgccga cgatgttgga tgacacctgg ctggtgccgc
      961 ctgcagcgtg ctggatgttg cgggcgatct cgcgcgtcgc cgcgccctgc tcttcgaccg
     1021 ccgccgcgat cgcggtcgtg acgtcgttga tctcgccgat ggtctggccg atatttctga
     1081 tggcgttgac ggcggaggtc gtcacggcct gcatgttggc gatctgcaag cgaatctcct
     1141 cggtcgcctt tgcggtctgg ttcgccaggc tcttcacttc ggaggcgacc acggcaaagc
     1201 cgcgtccggc ttcgccggcg cgcgccgctt cgatggtggc attcagcgcg agcaggttgg
     1261 tttgcgacgc gatcacctgg atgaggtcga ccacgaccga gatgcgactc gcattgtcgg
     1321 cgagcccctg catggtggcg tcggtcgcgc tggcttcggc gacggccttg cgcgcgattt
     1381 cggacgaact cacaacctgc cggccgatct cggtgatcga cgacgacagt tcctcggtgc
     1441 ccacggagac ggtctggaca ttggccgagg tctcttcagc cgcggagacg acggtgctca
     1501 ccagcgcgtt cgactggtcg gcggtggtcg acatgctctg cgccgtgctc tgcatggaat
     1561 tggcggcgtc ctgcagattg tcgagcgcgc agcgtacggt cgcctcgaat tcggcgatcc
     1621 gcgcttcgat gcgcgaggtg cgttcgatct tggcggcgcg gtccctgtcc tgctcggccg
     1681 agagggcgcg cgcctcgatc atgctctcgc ggaagacctg cagcgcgttc gccatcgagg
     1741 cgacctcgtc cttttggtgg ctctgataga tctcggattc gaggtcgccg tcggacaaaa
     1801 gctgcatcga gcgttgcagg ctgccgatcc gccgcaggat gttgccgccg acataacgcc
     1861 agacgaacag gaccgaaccg atcagggtcg ccgctcccat caccagcatg atcgtggtcg
     1921 cgaacgaaat ctgctggcgc gtcatgaagg tcgatgccgc ggttcccttc tgcacgtcgt
     1981 cgacgagctg ctgcacgctg acgtcgagac cgacgttgag cttgcgggtc tcttccagca
     2041 cggtctggcc gtaatcctcg gcatcgagct ccttctggcg cagcgagaat acgccgccct
     2101 taccctcgcc gagcgcgagc agttttgccg ctgcctcccg cagggcgttg gtcccgcggt
     2161 tactcggcag cagatcgagg ttgctcctca cgcgcccctg cgccgacttg tactcccttg
     2221 caatgtcgtc gagcttgtcg ctcgaacccg ccgacagcgc cgcgatcaga tcggccgcgg
     2281 cgagatttcc gctcgcgacc acattgccga gctggtcgac ggtttgagct gcctcgatag
     2341 cgtcagcggc ggagagactg gccgagccga gaatggcggt gatccgggtc tgggcgtcca
     2401 gcatcgcagg acttgcggcg gcgacgaaag ctgcctgcgc gctgcgcagc gcgtcatatt
     2461 ggttgtcgtg ctcggcggcg agcgtcactc gttcgcgggc ggccgagatc aggctctgcg
     2521 ccgcttcctg gatgttcttg atggtttcgc tgagcgcgga gatgacccgc ttgtcgacgc
     2581 cgagttcgat gatctcgcca agcttctcct gggtgacctc ctggatttcc ttcatcttct
     2641 tgatgcgttg gttcagcgca tcctcgtttt gcgaggcgag caccgcagga ccctgggccg
     2701 ccagcgtcgc ggcttgcgtc gacaattgca ggcttgcggc aaggcgggga atgtcctggc
     2761 cggacaggtc gaccatggtg tccccgaggt ggcgcagcac gaagccggcg ccggccgaga
     2821 tgacgatcgc catgccggcg atcgcggcaa aggcggcaaa caggctgccc ctcacgcccc
     2881 aaccccgccg caagaagcca gggattttgc cgtgacccca ccgaaccgcc atgcttccgc
     2941 ccccgcgacc cgaagatgaa ttgcgggcgg aagtatcgcg gtacgcggtt aacaaaatcg
     3001 ggaaagtctt accggttcgt tcgattttaa actcaaaaag ccgaagggcc ggtattccgc
     3061 cggcccttcg gacttgaacc ggaaagcggg atcaatagcg gccaacgagc gggccgcccc
     3121 agttgatccg gtaatttacg ccggccctga agacgttgtc gttggtcgtg aagttgccgg
     3181 tcgcaaagag cgcggcgggc gccacgaaat gcgtgtcgcc gaaattgtaa tattgatatt
     3241 ccgccttgac cgaccagttc ggcgcgaaca cgtattcgac gccggcgccg atggtgtagc
     3301 cgttcgtatg atcgcccgcg gtcgcgaacg cgaccggaat gccgccgagc gtcacgctct
     3361 cgttgttgtc ggaataggcg taaccgcctt tcgcatagac catcccgggg ccccaggtgt
     3421 agccgacgcg gccggtcagc gagcccagcc cgcgctggtt gttgttgtag atgaatccgc
     3481 cggggaagac cgcggcgacg ccgccggaca gccagctata ctggccctcg acgccgacca
     3541 cccagttggg gttgaactgc cagtcgtaac cggcctgcaa gccgccgagg acgcggccgt
     3601 tggaattgtt gccgatggca aggccagaga aattgttgtc gctggagaac gcgccgccga
     3661 cgtgagcgcc gatatagaag ccggtccagt tgtagaccgg cgcgacatag gcgggtgtcc
     3721 tgttgtaa
//
LOCUS       DATLJT010001487         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3122843,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001487 DATLJT010000000
VERSION     DATLJT010001487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3122843"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VM910_34070"
     CDS             complement(<1..200)
                     /locus_tag="VM910_34070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVI67573.1"
                     /translation="MRVASIGECMVEFSQHRDGLFARGFGGDTLNTALYLSRLGIATS
                     YVTALGDDGLSEAMLASWRDEG"
     gene            complement(211..1242)
                     /locus_tag="VM910_34075"
     CDS             complement(211..1242)
                     /locus_tag="VM910_34075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HVI67574.1"
                     /translation="MRAVVIHAPKDLRIDNFSDTAPAAGEVRVKIATGGICGSDLHYY
                     HHGGFGTVRIREPMALGHEIAGVVAEVGAGVLHLAPGTRVAVNPSRPCGVCIHCRGGM
                     RNQCLDMRFMGSAMRNPHVQGGFREHVTVEAAQAIPIAERLSLAEAAMAEPLAVCLHA
                     ANQAGPLMGKRVLVTGCGPIGALMVLVARCSGAAEIVVTDVAEAPLAVARQLGASHAI
                     NVTVQAAALDPFRAGKGVFDVLFEASGNQAALRLAFDLLRPGAVIVQLGLGGEMTLPV
                     NSIVTKELELRGTFRFDPEFELALRLMDAGLLDVKPLITASLPFEHAVDAFELATDRS
                     KSMKVQLTF"
     gene            complement(1421..2188)
                     /locus_tag="VM910_34080"
     CDS             complement(1421..2188)
                     /locus_tag="VM910_34080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67575.1"
                     /translation="MTLSLFDLSGRTALVTGSSRGLGRAIAEGMAKAGARLIVNGTDP
                     ARVETAIAEFRSAGHLAEGVAFDVTDEAAVVRAFEGLDAKGIAIDVVVNNAGIQMRKP
                     LIEFTSAEWRKVIETNLTSAFIIGREAAKRMIPRKRGKIINIGSLGSELARPTVGPYT
                     AAKGGIKTLTRSMAVEWAASGIQANAIGPGYMLTDMNQALVNNADFNNWLMSRIPAKR
                     WGRPDELIGAAIFLASSASDYVNGQIIYVDGGMLAAM"
     gene            complement(2198..>2498)
                     /locus_tag="VM910_34085"
     CDS             complement(2198..>2498)
                     /locus_tag="VM910_34085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI67576.1"
                     /translation="TEGRKIAAMAETYHRPFAPHDCIGPVGFIAAIHMSFSQPNTLIQ
                     ESVRAFYKGWYNELVTTMPVIKDGYVFPMEGPGLGVDLLPAVFKRPDLTVRRTNA"
BASE COUNT          488 a          792 c          766 g          452 t
ORIGIN      
        1 atgccttcgt cgcgccatga ggcgagcatg gcttcgctca agccatcgtc gccgagtgcc
       61 gtcacatagg aggtcgcaat gccgaggcgc gacagataga gcgcggtatt gagcgtgtcg
      121 ccgccaaacc cgcgcgcgaa cagtccgtcg cggtgttgcg agaactcaac catgcattcg
      181 ccgatcgagg ctacgcgcat cgaatgtttc tcaaaacgtc aattgcacct tcatcgactt
      241 cgagcggtca gttgccagct cgaaggcgtc gacagcatgc tcgaacggca aggaggccgt
      301 gatcaggggt tttacgtcaa gcagtcccgc atccatcagc cgcaaagcca gctcgaattc
      361 gggatcgaac cggaaagtgc cgcggagttc gagctctttg gttacgatgc tgttgacagg
      421 cagcgtcatt tccccgccga gcccgagttg gacgatgacc gcgccaggcc gcaatagatc
      481 gaatgcgagg cggagcgcgg cctggttgcc cgacgcctcg aacagcacgt cgaacacccc
      541 cttgccggcg cggaagggat cgagtgcggc agcttgcacg gtgacgttga tcgcgtgact
      601 ggcgccgagc tggcgggcca ccgcaagcgg cgcttccgcg acatcggtca ccacgatttc
      661 cgccgccccc gaacagcgcg caacgagcac catcagcgcg ccgatcggac cacagccggt
      721 caccagcacg cgcttgccca tcagtggtcc agcctgattg gcggcgtgca ggcagacggc
      781 gagcggctca gccatcgcag cttccgcaag ggaaagcctt tccgcgatcg ggatcgcctg
      841 cgccgcttcg accgtgacat gctcgcgaaa accgccctgc acatgggggt tgcgcatcgc
      901 gctgcccatg aagcgcatgt cgagacattg gttgcgcatc cctcccctgc agtgaatgca
      961 aacgccgcaa ggacggcttg gattgactgc gacgcgcgtg ccgggcgcga gatgcaggac
     1021 accagctccg acctccgcga cgacgcccgc gatttcatga ccgagcgcca tcggctcgcg
     1081 gatacgcacg gtgccgaagc cgccatgatg gtagtaatgc aggtcggagc cgcagatgcc
     1141 gcctgtcgcg atcttcacac gaacttcgcc ggcggccggc gcggtgtccg aaaaattgtc
     1201 aatccgcaga tctttcggtg catgaatgac aacggcgcgc atgacaatcc tcccaccagc
     1261 cccggttccg aagtttgcat tgtctcgcaa catcccgttt gcgacttcgg acataagaca
     1321 ttagcaactt tataatccag tacggcgttg gttcagaagt ccgcacgacg gatttgcggc
     1381 cattgtgatg cggacttccg aaccgccgga ctggccgcag tcacatcgca gccagcatgc
     1441 ccccgtcgac gtaaatgatc tgcccgttga cataatcaga tgcggacgaa gcgagaaaga
     1501 tggctgcgcc aatgagctca tccggacggc cccaacgttt cgcggggatc cgcgacatca
     1561 gccaattatt gaaatcggca ttgttgacaa gcgcctggtt catgtcggtc agcatatagc
     1621 cgggcccgat cgcattggcc tggattccgc tcgccgccca ttcgaccgcc atcgagcgcg
     1681 tcagcgtctt gatgccgcct ttggctgcgg tatagggccc gaccgtcggg cgggcaagct
     1741 cgctccccag cgaaccgata ttgatgatct tgccgcgctt gcgcggaatc atgcgctttg
     1801 ccgcctcgcg gccgatgata aaggcgctgg tgaggttggt ctcgatcacc ttgcgccatt
     1861 cggcgctggt aaattcgatc aggggttttc gcatctggat gcccgcattg ttgacgacaa
     1921 cgtcgatcgc gatgcccttc gcgtccaagc cctcgaaggc tctgacgacg gctgcctcgt
     1981 cagtcacgtc aaaggcgacg ccttcggcca ggtgaccggc cgatcgaaat tccgcaattg
     2041 ccgtctcgac gcgcgcggga tcggtgccgt tgacaatgag ccgcgcaccg gccttggcca
     2101 tgccttcggc gatggcgcgg ccgagaccac gcgaggagcc agtcacgagc gccgtgcggc
     2161 cggagagatc gaacagcgag agcgtcatgg caatccctca agcgttggtg cggcgtacgg
     2221 tgagatcggg gcgcttgaac accgccggca acaggtcgac gccgaggccc ggcccctcca
     2281 tgggaaaaac atagccatcc ttgatcactg gcatcgtggt gacaagctcg ttgtaccagc
     2341 ccttgtagaa ggcgcgcacc gattcctgga tcagcgtgtt cggctggctg aacgacatgt
     2401 ggatggcggc gatgaagccg accggcccga tgcaatcatg cggcgcaaac ggccggtgat
     2461 aggtctcggc cattgccgca attttgcggc cctcggtc
//
LOCUS       DATLJT010001488         6703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_134034,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001488 DATLJT010000000
VERSION     DATLJT010001488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134034"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VM910_34090"
     CDS             complement(<1..221)
                     /locus_tag="VM910_34090"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI67577.1"
                     /translation="MPLWKIYHPAGAYTAEDKKKLSQNITKVYTSIPIPAFYVVTIFE
                     DIAKDSCFVGGEPHNKFVRFKIDQIARTLP"
     gene            complement(360..1571)
                     /locus_tag="VM910_34095"
     CDS             complement(360..1571)
                     /locus_tag="VM910_34095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI67578.1"
                     /translation="MSAAPLQGLRVVDFTRVLAGPHCTKALRDLGADVAKIEPPSGDL
                     GRVGLPYVNGIGLYYVQQNAGKRNLSLDLNYAEAREIVTKLCRDADVIVENFRPGTLA
                     RFGFGYEDIKAYNPGIIYVSLSGYGQSTSWRNRPAFAPTVQAETGLTAIVQDHFGEAL
                     CEPRGDSCSHADVYTGLQGVIAVLAALQHRNRTGEGQHVDVSMAATMLSVNERAGAML
                     SEIDTDGEPIALSANESHIYEMADGTRLTIAGSPIYSPMFMRYCAMMRRSDLLTDPRY
                     ATAKLRRENLASLLAEIRAWLATFNDLDQLQAQVSEAGLAVGRVRTVNEFAKSDWVKE
                     WNAVVEVDDRAGGTLRMPGNPWIFSRSELPPPGAPAYQGEHNEEILKGLQIPEDRIRD
                     LEGRNIILNRR"
     gene            complement(1574..2341)
                     /locus_tag="VM910_34100"
     CDS             complement(1574..2341)
                     /locus_tag="VM910_34100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI67579.1"
                     /translation="MTTVNHEIDGKVGLVTLAKPPHNLLDDKLIGDLVAAYQAVVAEG
                     CRAILLRSSMRHFCAGAEMASWGTGTRIHTDQAKFEAMLRELEDVGVPTVAAVNGGAL
                     GGGLEVALTCDIIIAANTAFLGQVEVAVGLLPLLGGTQRLSQRAGVARAKEIAMLGRR
                     HTPEAFERWGIVNLVVPESELGTVSLSWARQLAAGPTQVIKGIKMQANLQARGGIAAA
                     DARQVEINDMIWEAKDRQRGAEAFFATGPATAVFQGD"
     gene            2528..3586
                     /locus_tag="VM910_34105"
     CDS             2528..3586
                     /locus_tag="VM910_34105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI67580.1"
                     /translation="MKPIKLVTPISGDPDAPTQNLLGLGALAAELAAEGVSVRDLFAR
                     TGVDASQLEDAHARISHRQRLALYRNAKKLAKRSDIGLLAGARQRISDYGIYGYAMVS
                     SRTFGDALLFSLDHVTMAGPAVRQISFRMEGTTAILRSHGLDTLGDLLPFAAEFWRSS
                     MTALFSRVLEAPFPSKRMIFPFPPPVHWRNYERLFNCSIDFNADRMEWHFDAKVLELA
                     CPNANPITAKICQQVCDVVMTERPGESEFVRRIRAACLNSPSRFPAAAEIASELGLSL
                     RTFHRRLADENLSYQSIVDEMRRSLATELLENTHMGIDQVAERVGFADATSFRKAFKK
                     WTNHAPSDFRPSERSRGT"
     gene            complement(3583..4914)
                     /gene="fahA"
                     /locus_tag="VM910_34110"
     CDS             complement(3583..4914)
                     /gene="fahA"
                     /locus_tag="VM910_34110"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVI67581.1"
                     /translation="MTKTTGLDETHDPARRSFVEAANRPETDFPIQNLPLGVFSTKAD
                     SSLRIGVAIGDQVLDLKRASGRSERLTGSTREALQQTTLNRLFALGSGALRDLRRTVA
                     DMVDRGASGNETRRHAADLLTPMEVCSFRLPTEVCNYTDFYAGIYHARAAGALLTPEN
                     PLPQNYKWVPIAYHGRASSVQVGRGTIRRPLGQRPPSAAGAPPSFGPCERLDFELEMG
                     FYLAGGNRLGRPIPIAEASQEIAGFTLLNDWSARDIQRWEMYPLGPFLSKSFATSVAP
                     WVVTADALAPFRVGALPRPDGDPRPLPYLSDAADQASGGLDVHLQVLLSTAQMRRANE
                     PAVEILTSNAKYLYWTPAQMVAHHTVNGCNLQPGDLIGTGTISGPSPAELSSMLEFTT
                     AGTKPVMLANGEKRGFLEDGDEVTFRGRCSREGFAAIGFGSCTGRIEGAAF"
     gene            complement(4949..5488)
                     /locus_tag="VM910_34115"
     CDS             complement(4949..5488)
                     /locus_tag="VM910_34115"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HVI67582.1"
                     /translation="MALAPITGSLTASTTERLEITSTPLPVEHIIIKTMKPYSEVKAG
                     IEKLGRLDDSIRSLLTKNDIEGLRTALKRIAGEDGLAIHYVALHGDLLALKGERRPLT
                     AYYIGNVLSAVEMTKVNPASGLYAPLRVVVYANVEGGTTIEYDRPSSMFGQFKNREID
                     AMAQSLDQRLMKFLKKVSM"
     gene            complement(5578..>6703)
                     /gene="actP"
                     /locus_tag="VM910_34120"
     CDS             complement(5578..>6703)
                     /gene="actP"
                     /locus_tag="VM910_34120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822922.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HVI67583.1"
                     /translation="LMILYVTVGGMQATTWVQITKAALLLCGGTLLGITVLWHFDFSF
                     AELFAAASAIHPKGAAILAPGGLFSEPVSAISLGLALVFGTAGLPHILMRFFTVKDAA
                     GARKSVFYATTCVAYFCVVIPVLGFGAVAILMSDPAYFHVGASGQFNKIADLIGGPNM
                     AAIHLAHAMGGPILFGFMAAVAFATILAVVAGLTLAGASAVSHDIYGQVIARGQASDT
                     REVWVSKLAAIAIGIIAVLLAYAFENQNVAFMAGLALSVAASCNFPVLVMAMFWRGTT
                     TRGAMAGGLVGLAASVVLVLLSKTVWVAVFHFPTAIFPYENPALFSMPLAFLFAWLFS
                     VTDRSGRAVRERAAFDAQFVQSEIGAAQTIDSSKLAAAAP"
BASE COUNT         1262 a         2054 c         2098 g         1289 t
ORIGIN      
        1 ggaagcgtgc gcgcgatctg gtcgatcttg aaacgcacga atttgttgtg cggttcgccg
       61 cccacaaagc aggagtcctt ggcgatgtcc tcaaagatgg tcacgacgta gaacgccggg
      121 attgggatgc tggtgtagac cttcgtgatg ttctgggaga gtttcttttt gtcctctgcg
      181 gtgtaggcgc cggccgggtg gtagattttc cataagggca tgatgtttcc tcaaaagttt
      241 ggtgattttt ttagttgggc gaatagttgt ctctgatgtg ccgtttccat caatccatgg
      301 cgcctccctc ggttgaataa gcaatcggcg atcagcgcgc gacaagtcga tcggggcggt
      361 caccgcctat ttaaaatgat gttgcgccct tccaggtctc tgatcctgtc ctcggggatc
      421 tgtagtcctt tgaggatttc ctcattatgt tcgccctgat aggctggcgc accgggaggc
      481 ggcagctcgg atcggctgaa gatccagggg ttgcccggca tgcgcaaggt gccgcccgcg
      541 cgatcgtcga cctccacgac cgcgttccac tccttgaccc aatcggactt cgcgaactcg
      601 ttgacggttc gcacccgacc aaccgcgagg cctgcttcgc tcacctgcgc ctgcaactgg
      661 tcgagatcgt tgaacgtcgc gagccacgca cggatctcgg ccagcaatga tgcgagattt
      721 tcccggcgca gtttcgccgt cgcatagcgc ggatcggtga gtaggtcgct tcgccgcatc
      781 atggcgcagt agcgcatgaa catcggcgaa tagattgggc ttccggcgat ggtcaatctt
      841 gtgccgtcgg ccatttcgta gatgtgggac tcgttggccg acagcgcgat cggctcgccg
      901 tcggtgtcga tctccgatag catggcgccg gcgcgttcgt tcactgacag catcgtggcg
      961 gccatcgaca cgtcgacatg ctggccctcg ccggtgcggt tacgatgctg caaggccgcg
     1021 agcacggcga tgacgccttg cagcccggtg tagacatccg catggctgca ggaatccccg
     1081 cgcggttcgc aaagcgcctc gccgaaatga tcctgcacga tcgcggtcag gccggtttcg
     1141 gcctgcacgg tcggcgcgaa ggcgggacga tttctccatg aggtcgattg tccatagccg
     1201 ctcagggaga catagatgat gccgggattg tacgccttga tgtcctcgta gccgaagccg
     1261 aaacgggcaa gcgtgccggg acggaaattc tcgacaatga cgtcggcgtc gcggcagagc
     1321 tttgtaacga tctcgcgcgc ttcggcgtag ttgagatcga ggctgagatt cctcttgccg
     1381 gcgttttgct ggacgtaata gagcccgatg ccgttgacgt aaggcaggcc cacgcggccg
     1441 aggtcgccgg acggcggctc gatcttggcc acgtcggcgc cgaggtcgcg cagcgccttg
     1501 gtgcaatggg ggcccgccag cacgcgggta aaatcgacca ccctcaatcc ctgaagcgga
     1561 gcagcggaca tgatcaatct ccctgaaaaa ccgcggtcgc ggggccggtg gcaaagaagg
     1621 cctcggcgcc gcgctgccgg tccttggctt cccagatcat gtcgttgatc tcgacctgcc
     1681 gggcatccgc cgccgcgatc cccccgcgcg cttgcagatt ggcctgcatc ttgatgccct
     1741 tgatcacctg tgtgggtccc gccgcaagct gccgcgccca ggacagcgac accgtgccga
     1801 gttcggattc gggaacgacg agattgacga tgccccagcg ctcgaacgct tcgggggtgt
     1861 gacggcggcc gagcatggcg atttccttgg cgcgcgcgac gccggcccgt tgcgacagcc
     1921 gctgggtgcc gccgagcagg ggcaacagtc cgaccgcgac ctcgacctga cctaagaacg
     1981 cggtgttggc ggcaatgata atgtcgcagg tgagcgcgac ctcgaggccg ccgccgagcg
     2041 ctccgccatt gacagctgcc accgtcggca cgcctacatc ctcgagctcg cgcagcattg
     2101 cctcgaattt cgcctgatcg gtatggatgc gcgtaccggt tccccatgac gccatctcgg
     2161 cgccggcgca aaaatgccgc atcgaactcc gtagcaggat cgcccggcag ccctcggcca
     2221 cgaccgcctg ataggcggcg acgagatcgc cgatcagttt gtcgtcgagc agattgtgcg
     2281 gcggctttgc cagcgtgacc aagccgactt tgccatcgat ttcgtggttg actgtcgtca
     2341 tcgtgatccc ttggtttcct cgttttgctt tgggcttcgg gctatttgcc gacgcgtgca
     2401 agcttggccg cattcaaaaa aggtgacaac gtcaagcgct gccaattata atgtcaaaga
     2461 ctgccacaaa gacaaaagcg gccggccttg gcaggtcaat ctgtgccaag gtgtctggga
     2521 ggacgagatg aagccgatca agctcgtgac gccgatctca ggcgatcctg acgcgccgac
     2581 gcagaacctt ctgggcctag gagcgctggc cgccgagctt gcggcggagg gggtttcggt
     2641 ccgcgatctc tttgcgcgaa ccggcgtcga tgccagccag ctcgaggatg cgcatgcgcg
     2701 catctcgcac cgacagcggc tggccctcta ccgcaacgcc aagaagctcg ccaagcgctc
     2761 tgacatcgga ctgctcgccg gagcgcggca gcgcatcagc gactacggca tctacggcta
     2821 cgccatggtc tcgagccgaa ccttcggcga tgcgttgttg ttctcgctcg atcatgtcac
     2881 catggcaggc cccgctgtcc gccagatcag tttccggatg gaggggacta cggcgatcct
     2941 gcgcagccat ggcctcgaca ctctcgggga cctcttaccc tttgcagccg aattctggcg
     3001 aagctcgatg accgcgctgt tcagccgtgt cctggaagcg ccattcccta gcaagcggat
     3061 gatctttcct tttccgccgc cggttcattg gcgcaactac gaacggctgt tcaactgctc
     3121 gatcgatttc aacgccgacc ggatggaatg gcattttgac gcaaaggtcc tcgagctggc
     3181 ctgcccgaac gcaaacccaa tcacggcaaa aatctgccag caggtctgcg atgtggtgat
     3241 gactgagcgg cccggcgaat ccgagttcgt gcgcagaata cgtgccgcgt gcctcaacag
     3301 ccctagcagg tttccggccg cagccgagat cgcgtccgaa ctaggcttgt cgctgcggac
     3361 ctttcatcgg cggttggccg acgagaacct gtcgtatcag tccattgtcg acgagatgcg
     3421 ccgctcgctt gcgaccgagc ttctggaaaa tacacatatg ggtatcgacc aggttgccga
     3481 gcgggtcggc tttgccgacg cgaccagttt tcggaaggcg ttcaagaagt ggaccaacca
     3541 tgcgccctcg gatttccgtc cctcggagcg cagtcggggc acctagaatg ccgccccctc
     3601 aatcctgccc gtgcaggatc cgaagccgat ggccgcaaag ccttcgcggc tgcatctgcc
     3661 gcggaaggtg acctcgtcgc cgtcctcgag gaagccgcgc ttttccccgt tcgcaagcat
     3721 tacgggtttc gtcccggcgg tcgtgaactc gagcatgctg ctgagctcgg ccgggctcgg
     3781 ccccgaaatg gtgccggtgc cgatcagatc gcccggctgc agattgcaac cgttcacggt
     3841 gtgatgggcg accatctgcg cgggggtcca gtagagatac tttgcgttcg aggtgaggat
     3901 ttcgacggca ggttcgttcg cacggcgcat ctgtgcggtc gacagcagaa cctggagatg
     3961 aacgtcgagc ccgccgctcg cctggtcggc cgcatcggag aggtagggaa gcggccgcgg
     4021 atcgccatcc ggacgcggca gcgcgccaac gcggaagggc gcgagcgcgt ccgcggtaac
     4081 gacccagggt gcaaccgagg ttgcaaagct tttgctcagg aagggtccga gcgggtacat
     4141 ctcccagcgc tggatgtcgc gcgcagacca gtcgttcaac agcgtaaagc ctgcgatctc
     4201 ctgcgaggcc tccgcaatcg ggatcggcct gccaagtcga ttgccgccgg cgaggtagaa
     4261 tcccatctcg agctcaaaat ccagccgttc gcatgggccg aaactcggcg gcgcgccggc
     4321 cgcactcggc ggtcgttgcc caaggggccg ccgtatcgtg ccgcgcccga cttgcaccga
     4381 ggaagcgcga ccgtgatagg cgataggcac ccatttgtag ttttgcggca gcgggttttc
     4441 tggcgtcaat agagcgccgg cggcgcgcgc atgatagata ccggcataga aatccgtgta
     4501 attgcaaacc tccgtcggca gacggaacga gcatacctcc atcggcgtca agaggtcggc
     4561 ggcatgccgt cttgtctcgt tgccgctggc gcccctgtcg accatgtcgg cgacggtgcg
     4621 gcgcaaatcg cgcagcgcgc cggagccgag cgcgaaaagc cgattgaggg ttgtttgttg
     4681 aagggcctcg cgcgtcgatc cggtcaagcg ttcgctgcgt ccgcttgctc gtttgagatc
     4741 caggacctgg tcgccgattg ccacgccaat gcgcaggctg ctatcggcct tcgtgctgaa
     4801 cacgccgagc ggtaggttct ggatcggaaa atccgtctcc ggccggtttg ccgcttccac
     4861 aaagctgcga cgcgccggat cgtgtgtctc atcgagacca gtagttttgg tcatggcgtc
     4921 tccgatcgac cgccgtcgcc tggcggtcct acatgctgac tttcttcagg aacttcatca
     4981 atcgctgatc gagcgactgt gccatggcat cgatctcccg attcttgaat tgtccaaaca
     5041 tcgagcttgg ccggtcatat tcgatcgtgg tgccgccttc gacgttagcg tacacgacca
     5101 ctcgtagcgg cgcatagaga ccggacgcag gattgacctt ggtcatctcc accgcggaca
     5161 gcacattgcc gatatagtag gcggtgagcg gccggcgttc tcccttcagt gccaaaaggt
     5221 cgccgtgaag tgcaacataa tggatcgcaa ggccatcctc gccggcgatc cgcttgagcg
     5281 cggtgcgcaa tccctcgatg tcgtttttcg tcagcaacga gcggatgctg tcgtcaagcc
     5341 ggccgagctt ctcgatcccg gcctttactt ccgaataagg cttcatcgtc ttgataatga
     5401 tgtgctcgac cggaaggggt gtcgacgtga tctcgaggcg ctcggtcgtc gaggcggtga
     5461 gggagccggt tattggagca agggccatgg ccaaaagtcc tcccaagatc gagccgcgaa
     5521 gccgtcgaac gctgcgtgtc atcgctagtc ctctctgttg atggttagcg cgtgctgtca
     5581 gggtgcagcg gcagccagtt tggaactgtc gatcgtctga gctgctccga tttcagactg
     5641 gacgaactgc gcatcgaacg ccgcacgttc gcgaaccgca cgcccgcttc ggtccgtcac
     5701 cgagaacagc caggcgaaca ggaaggccaa tggcatcgaa aacagcgcag gattttcata
     5761 gggaaagatc gcggttggga aatgaaacac ggcgacccaa accgtcttgc taagcagcac
     5821 gaggaccacg gaggcggcga ggcccaccag gccgcccgcc atggcgccgc gggtagtggt
     5881 gccgcgccag aacatcgcca tcaccagcac cggaaagttg cagcttgcag caaccgacaa
     5941 tgcgagcccg gccatgaacg ccacgttctg gttttcgaag gcataggcga gcagcaccgc
     6001 gataatgccg atcgcaatgg cggccagctt cgagacccaa acctcgcggg tgtccgaagc
     6061 ctgtcccctc gcaatcacct ggccatagat gtcgtggctg accgcagacg caccggcaag
     6121 ggtcaacccg gcgaccaccg cgaggattgt cgcgaaggcg acggcggcca tgaagccgaa
     6181 caggatgggg ccgcccatcg cgtgcgccag atggatcgca gccatgttcg ggccgccgat
     6241 cagatcggcg atcttgttga actgcccgga ggcaccaaca tggaaatagg cggggtcgga
     6301 catcaggatg gctacggcgc caaatcccag aacggggatg acaacgcaga aataggccac
     6361 gcaagtggtg gcatagaaca ccgatttgcg tgcgcccgca gcgtccttca cggtaaagaa
     6421 gcgcatcagg atgtgtggga gacctgccgt tccgaacacc agcgcgagcc ccagcgaaat
     6481 cgccgatacc ggttcggaga acaagccgcc aggcgcaaga atcgccgcgc ctttcggatg
     6541 aatcgcgctg gctgcggcga acaattcggc gaagctgaaa tcgaaatgcc acaatacggt
     6601 gatgcccagc agtgttccgc cgcagagcag cagcgcggcc ttggtgatct gcacccacgt
     6661 cgtggcctgc attccgccga ccgtgacgta gaggatcatc agc
//
LOCUS       DATLJT010001489         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1285938,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001489 DATLJT010000000
VERSION     DATLJT010001489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285938"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VM910_34125"
     CDS             complement(<1..440)
                     /locus_tag="VM910_34125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67584.1"
                     /translation="MSDLDKALADISAIRSQLAAGTAFRGYGPAAIAATSAVALITAI
                     LQYLFLDDPTHNPLTFLFGWAAAAALSGAMIWTEMVARTRRHHSGLADAMLYQALEQF
                     LPAGIAGVLLAVTLWKFAPETLWLLPGLWQVLTSLGVFASVRSLP"
     gene            680..1924
                     /locus_tag="VM910_34130"
     CDS             680..1924
                     /locus_tag="VM910_34130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVI67585.1"
                     /translation="MFRGVIVACLFQAAVASMAHAAGPFGKIHVGNWSGGAFSDDNTG
                     GFSHCAASSGFASGVILVVGQNAANSWLLGFASPAFHLKKGDTYPIDVTFDGQSEAKL
                     FANATSDIMLTAILPPGVARTFQKASLMVAVAGPTTLQFSLTSTGPLLAVLATCVSKI
                     KAEGLNAAGDFTAAKAVAAKPGATTDAPGASSKAAKSVSKSGTGFVISASGHIVTNQH
                     VIDGCTDIKGNLGGQPPATLRVVSSDETNDLAVLQGPAATFKDFAKIRDRPIRSGDSV
                     VAIGYPFHGLLSSDFTVTTGIVSSLSGTMNDTRQLQISAAIQPGNSGGPLLDTGGLIV
                     GVVAAKLDALRVARVTGNIPENINFAIKTGAIRDFLDNSVVAYETASPVGELKTAEIA
                     NNARAFTLLITCTAKEPVEVKR"
     gene            complement(1968..2719)
                     /locus_tag="VM910_34135"
                     /pseudo
     CDS             complement(1968..2719)
                     /locus_tag="VM910_34135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083667.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
BASE COUNT          502 a          888 c          840 g          489 t
ORIGIN      
        1 ggcaacgagc ggaccgacgc gaacacgccg agcgaggtga gcacctgcca caggccgggc
       61 aacagccaca gcgtctccgg cgcaaatttc cagagcgtca ccgcaagcag gacacccgcg
      121 atgcccgccg gaagaaactg ctcgagcgcc tgatacagca tggcatcagc gagacccgaa
      181 tgatgccggc gcgtgcgcgc caccatctcg gtccagatca tcgcacccga caatgctgcc
      241 gccgcggccc agccgaacag gaatgtcagc ggattgtgag tgggatcgtc gaggaagaga
      301 tattgcagga tggcggtgat gagcgcgacg gcactggttg ctgcgatcgc cgcgggcccg
      361 tagccgcgga atgccgtgcc ggctgcgagc tggctacgga tggcgctgat gtccgccagc
      421 gccttgtcga gatcgctcat cgcgccgcct gcctcccctg gctttgcgtc gcaaagtata
      481 gggaaaggcg aggcaaaggc aagcagacgc tcccgcgttt tgcaccggtt ccctcgaata
      541 gacctcttcg cacgcccaaa tcctccagat attgcgcatg ttaacaatac aaccgcaatg
      601 tcttgaaact accactggtt gctttgtggg cgcggggaac cgtagaatgc cccgccttgg
      661 gctggggagg aagcgtgcaa tgttccgggg ggttatcgtc gcgtgcttgt ttcaagccgc
      721 ggtcgcgtcg atggcgcacg ccgcaggccc gttcggcaag atccatgtcg gaaactggtc
      781 cggcggcgcc ttcagcgacg acaacacggg cggcttctcg cactgtgcgg cgtcgtcggg
      841 tttcgcaagc ggcgtcatcc tcgttgtcgg ccagaatgcc gcaaattcct ggttgctcgg
      901 ctttgccagc ccggcttttc acctgaagaa gggcgataca tatccgatcg acgtgacctt
      961 cgacggacag tcggaagcga aactgttcgc gaacgccacg tccgacatca tgctgacggc
     1021 gatcctgccg ccaggcgttg cgcgcacctt tcagaaggcg agcctgatgg tcgccgtcgc
     1081 aggaccaacc acgcttcagt tcagtctgac gtcgaccgga ccactgcttg ccgtgctcgc
     1141 cacctgcgtc agcaagatca aggccgaagg cttgaatgcg gcgggcgatt tcaccgctgc
     1201 gaaggccgtc gcagccaagc cgggcgcaac gacggatgca cccggcgcct cgtcaaaggc
     1261 cgcaaagagc gtgagcaaaa gcggcaccgg ctttgtgatc agcgcgagcg gccacatcgt
     1321 caccaatcaa catgtcatcg atggctgcac cgacatcaag ggcaatcttg gcggtcagcc
     1381 gcctgcgacg ctgcgggtcg tgtcgagcga cgagaccaat gatctcgccg tgctgcaggg
     1441 accggcagcc accttcaagg attttgcgaa aatccgcgat cgtccgatcc gctcggggga
     1501 ttccgtggtc gcgatcggct atcccttcca cggcctgttg agttcggatt tcacggtcac
     1561 caccggcatc gtcagttcgc tgagcggcac catgaacgat acgcggcagc tccagatcag
     1621 cgccgcgatt cagccgggca acagcggcgg cccgctgctc gataccggcg ggctcatcgt
     1681 cggcgtcgtc gcggcgaagc tcgatgcctt gagggtcgca cgcgtcaccg gcaacattcc
     1741 ggaaaacatc aatttcgcga tcaagaccgg cgcgatccgc gatttcctcg acaacagtgt
     1801 cgtcgcctac gagaccgctt cccccgtcgg cgagctcaag accgccgaga tcgcaaataa
     1861 cgcccgcgcc tttacgctgc tgatcacctg caccgccaag gagccggtcg aggtcaagcg
     1921 gtagcattga tcttgagccc cggcggcagg cggccccgct tgtcggatca ctcgaccttg
     1981 agcccggcga actccaccac cttccgccac ttctcggtct cggccttgat catgccgccg
     2041 aacgcctccg gcgactggat cagcggctcg ccgccgagct cgaccaggcg ctgcttcatc
     2101 tcgggctcgg ccagcaccgc attgatctcc gcgttgagcc tgccgacgat ctctctcggc
     2161 gtcttcgcgg gcgcgcccat gccgaacagc gcacttgcct catagcccgg gacggtctca
     2221 gcgatcgcct gcacgtcggg caattgcggc gaccgctccg ccgtcgtcac cccgagcgcg
     2281 cgcaacgcgc cggagcggat gtgctggatg atcgagggca tgttgtcgaa gatcacctgc
     2341 acctggccgc cgagcaggtc ggtgatggcg ggcgccgcgc cgcgataggg cacgtgctgc
     2401 atctggcagc cggtcatcgc catgaacatc tcgcccgaca gatgcaccga ggtgccgttg
     2461 ccggaggacg ccatgttgat cttgcccgga tttttcttca cgtaatcgat gaactcggcc
     2521 accgtcttcg ccggcacgtc cttgttgacc gtcatcacgt tgggcacgcg attgaacgat
     2581 gccacgggcg cgatgtcgtt gacgaaattg aacttgaggt tggcgtagag cgaggtgttg
     2641 atgtattcgc cgggttcacc agcagcaccg tatagccgtc gggctcggcg ttcaccgcag
     2701 cctcggtgcc gatattgtt
//
LOCUS       DATLJT010001490         5172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_822578,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001490 DATLJT010000000
VERSION     DATLJT010001490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822578"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VM910_34140"
     CDS             <1..1184
                     /locus_tag="VM910_34140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67586.1"
                     /translation="EQTSGEPGSVGSGSTVPTSPDPLIDTVMASMREASTTETAVITS
                     DHEAPPKEIPAKEDPPKAESPAIEIPAIEPPKMEATNVEATSVEATSVEATSVEATSV
                     EATKAEMPKIEVKAETPRTANKVLIMSSGRRTWHEEPQTSTAQAEPAQAEPAQAAPRQ
                     RRLVGMVALVALASAAGAIGGALATAGLAHVSDAAATASGNSTLDAAIARIDADIVAL
                     KAGLEQTSKIGMSQFNKTSDRLDKMEKAQTEPAAKLAKLSEAVEKLRAASAAPVVTAA
                     APVAPVAAAAPAAAPAAPKDGTGMITTPAGTAALPTPAASPAPAPSASPKPDVARLPK
                     VEGWVLRDVAHGGALIEGRNGLYEVYAGDPVPGLGRIDAIRKQDGHWVVVTSRGLVVA
                     R"
     gene            1312..2856
                     /locus_tag="VM910_34145"
     CDS             1312..2856
                     /locus_tag="VM910_34145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67587.1"
                     /translation="MSMLLVCGFSPAHGGEGAALERGTAIIDPAALRELDHGRFGLRR
                     IMAPMQSADVPLTSGDLFALPEMAPVKKALDEDFERYIERHKAELPTESIGVGQGFDF
                     QLFDRTQLSSADTRFVLSGIVNRMDRAYVSEKDCGEIRLIYRLTQTGAGAIGEAAVSP
                     RLPMTLNVVLKAKGAIPVDGAGVTITCAEIARRWLAAGQLAASGAELAGKLTATGGAL
                     DLVRPENIERIETNLQIAHAPKSAVRDFRTDYLMKVFDYDAASETFREAPLENQIDRA
                     RLLADENLSHDFKAWLLDLAHFAELDKGTILIPERFLVKSAVAATPVGFAPLELQPAF
                     GLVASEDGPTNPVFAESDVVGALKRATEHGAALQNIRSVAGFERRLNDMTCTGCHQTR
                     GIGGFHFPGVDWLAAKPSNVTVVPASPHFFGDQVRRRDILAAFRDGKRVDFSRGFASR
                     PQTRGSSELAGSEYENGWGAHCYVQHAKMSENDASFRSWTCADGLACQAVSPATRMGM
                     CFVKGR"
     gene            2934..4169
                     /locus_tag="VM910_34150"
     CDS             2934..4169
                     /locus_tag="VM910_34150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HVI67588.1"
                     /translation="MDEIIIVGAGIGGLTLGLALHQAGIACRIYESVPDVKAIGVGIN
                     LLPHATKELAALGLEDALARVAVKTRDATFFNRFGQLIYQEPLGRAAGYEHPQFSIHR
                     GDLQMILLEEFRSRTGGDRIVTNSHCVGVAQDEAGVTVTFSDGPGGKNRFAVRGRVAI
                     ACDGINSAVRKQFFPGEGEPRYSGVNMWRGVTRWKPMLSGASMVRAGWLSHGKMVIYP
                     IRDADTDGLQLINWVAEIETPNYRKRDWNRAGSIEDFIGAFADWHFDWLDVPAFVRAA
                     DLVLEFPMVDQDPLPRWSFGRVTLLGDAAHPMVPRGSNGAGQAILDVRALTTALREHA
                     DPVAALAAYEAQRLEATTRVVLTNRTNPPDAILREVYERTRDRPFAAIGDVISHEELV
                     ALSEGYKRIAGYSKDALRG"
     gene            4239..>5172
                     /locus_tag="VM910_34155"
     CDS             4239..>5172
                     /locus_tag="VM910_34155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67589.1"
                     /translation="MANTQADNSPAIDLVEFIDRQPVGGFQIRLLLLCAAVLFLDGFD
                     TQAIGYVAPAIAKEWGLTKGALGPVFSAGLFGLMIGALVFGPLADHIGRKKIIIFSTA
                     AFGVGALVTAFVHDVSALLVIRFLTGLGLGGAMPNTVAMTSEFNPQRRRATMVMIMFC
                     GFSVGAALGGLLAADLIPRFGWRAVFIVGGIAPLLMLPILVWRLPESVRFLATFGHAP
                     ERVAELLKRISPNTAFAPEARFVVHETHLAGLPVKHLFREGRSLATSLLWVVFFMSLL
                     DIYFLANWLPTVLNDLGASVSEAVLIGSLLQIGGV"
BASE COUNT          889 a         1636 c         1732 g          915 t
ORIGIN      
        1 ccgagcagac cagcggcgaa cccggttccg tcggtagcgg gtcgaccgtt cccacttccc
       61 ccgatccact gattgatacg gtgatggcat cgatgcgaga ggccagcacc accgaaacgg
      121 cggtgatcac ctccgaccac gaggccccgc cgaaagagat tcccgcgaag gaggatccgc
      181 ccaaggcgga gtcacccgcg atcgagatac ccgcgatcga gccgcccaag atggaggcga
      241 ccaacgtgga agcgacctcc gtggaagcga cctccgtgga agcgacctcc gtggaagcga
      301 cctccgtgga agcgaccaag gcggaaatgc cgaagatcga ggtcaaggcc gagacgcccc
      361 gcacagccaa caaggtgctg atcatgtcgt ctggtcgccg cacctggcat gaggagcctc
      421 aaacttcgac ggcgcaagcg gaacctgcac aagcggaacc tgcacaagcc gcgccgcgcc
      481 aacgccggtt ggtcgggatg gttgccctgg tggcgctcgc gagtgcggcg ggcgcgatcg
      541 gcggtgcgct ggcgaccgcg gggcttgcgc atgtttcgga cgccgcagcg acagcttcgg
      601 gcaacagcac tctcgacgcg gcgattgcgc ggatcgatgc cgacatcgta gcgctgaagg
      661 ccggcctcga gcagacctcg aagatcggga tgagccagtt caacaagacg agcgaccgtc
      721 tcgacaagat ggagaaagcc cagaccgaac ccgctgccaa gctcgcgaaa ctctcggaag
      781 ccgtcgagaa gcttcgcgcg gcgtcagccg cgcccgtcgt aacggcagcc gcaccggttg
      841 cgcctgttgc cgccgctgcc ccggccgcag caccggcagc gccgaaggac ggcaccggca
      901 tgatcacgac gcctgcgggg acggcggccc tgcctacgcc ggcggcttca cccgcgcctg
      961 caccatcggc ttcgccgaag ccggacgtgg cacgcctgcc caaggtcgaa ggctgggtgc
     1021 tgcgcgacgt cgcccacggc ggcgcgctga tcgaaggacg caacggcctc tacgaggtct
     1081 atgccggcga tcccgtgccc ggtctcgggc ggatcgatgc gatccgcaag caggacggcc
     1141 actgggtggt cgtgacctcg cggggccttg tggtcgcgcg ctagaggcac aaacaagtcc
     1201 tcgcggaatt ctttgaggcg tctcaccgca atgtgaggcg cctttctcct tgaataggcc
     1261 tcgttgcgcg gtgttagtgg gggcatgagc cgcgtgctgc gatcgctttt catgtccatg
     1321 cttctggtgt gcggcttctc gccggcgcac ggcggtgaag gcgcggcgct ggagcgcggc
     1381 accgcgatta tcgatcccgc ggcgttgcgc gagctcgatc acggccggtt cggcctcagg
     1441 cgcatcatgg cgccgatgca gtccgccgat gttcccttga cgagcggcga cctgttcgcg
     1501 ctgccggaga tggcgccggt gaaaaaggcg ctggatgagg atttcgaacg ctacatcgag
     1561 cgccacaagg ccgagcttcc aaccgaaagc atcggcgtcg gccaaggctt cgatttccaa
     1621 ctgttcgacc gcacccagct ctcttccgcc gatacgcgtt ttgtgctgtc cggcatcgtc
     1681 aaccggatgg accgcgccta cgtgtcggaa aaagattgcg gcgagatcag gctgatctat
     1741 cgcctgaccc agacaggggc gggggcgatc ggcgaggccg cggtgtcgcc gcggttgccg
     1801 atgacgctca acgtcgtgct gaaggccaaa ggcgcgatcc ccgtcgacgg cgccggcgtc
     1861 acgatcacct gcgccgagat cgcgcggcgg tggcttgccg cgggccagtt ggcggcaagc
     1921 ggggcggaac tcgccggcaa gctgacggcg accggcggtg cgctcgatct ggtcagaccg
     1981 gagaacatcg agcgcatcga aaccaatctg cagattgcgc acgcgccgaa gtcggccgtg
     2041 cgggattttc gcaccgacta cctgatgaag gtgttcgact atgacgcggc gtcagagacc
     2101 ttccgggaag cgccgctgga aaaccagatc gatcgcgcgc ggctgcttgc ggacgaaaac
     2161 ctgtcgcacg atttcaaggc ctggctgctc gacctcgcgc atttcgcgga gctcgacaaa
     2221 ggcacgatcc tgatcccgga acgattcctg gtgaagagcg cggttgccgc aaccccggtg
     2281 ggatttgccc cattggagct gcaacccgcc ttcggcctgg tcgccagcga ggacgggccg
     2341 acaaatcccg tcttcgcgga gagcgatgtc gtgggtgcgt tgaagcgcgc gacggagcac
     2401 ggcgcggcac tgcagaacat ccgctcggtg gcaggcttcg aacgtaggct caacgacatg
     2461 acctgcaccg gatgtcatca aacgcgcggc atcggcggtt ttcattttcc gggcgtggac
     2521 tggctcgcgg caaagccgtc caatgtgacg gtggttccgg cgtcgccgca ttttttcggc
     2581 gaccaggtcc gtcgccgcga cattcttgcg gcctttcgtg atggcaaaag ggtcgatttc
     2641 tcgcgcggct ttgccagccg accgcaaacg cgcggcagct ccgagcttgc cggcagcgag
     2701 tatgagaatg gctggggcgc gcattgctat gtgcagcatg caaagatgag cgagaacgac
     2761 gcgagcttcc gctcctggac ctgcgccgac gggctcgcct gccaggcggt cagcccagcg
     2821 acgcggatgg gcatgtgctt cgtgaaaggg cgctgacgct ttgcgggcga gattgacggt
     2881 agacttgcca atattgtttc atcgcttgga agaaggcttt gcgggacgga aacatggacg
     2941 agatcatcat cgtcggcgcc gggatcggtg gcctgacgct cggactggcg ctccatcagg
     3001 cggggattgc ctgccgcatt tatgaatccg ttcctgacgt gaaggcgatc ggcgtcggca
     3061 tcaacctgct accgcacgcg accaaggaac tcgccgcact cggactggaa gatgcgttgg
     3121 cgcgcgtggc ggtcaagaca agggatgcga cctttttcaa ccgcttcggg caattgatct
     3181 accaggagcc gctcggccgc gcggccggct atgagcatcc gcagttctcg atccatcgcg
     3241 gcgatctgca gatgatcctg ctcgaggaat ttcgatcccg cacgggaggt gaccgcattg
     3301 tcaccaacag ccattgcgtc ggcgtcgcgc aggacgaggc cggcgtgacc gtgaccttct
     3361 ccgacggtcc cggcggaaaa aatcgcttcg ccgtccgtgg ccgcgttgcg atcgcctgcg
     3421 atggcatcaa ttcggcagta cggaaacagt tctttcccgg cgagggtgag ccgcgttatt
     3481 ccggcgtcaa tatgtggcgc ggcgtgacgc gctggaaacc gatgctgtcg ggcgcgagca
     3541 tggtgcgcgc gggctggctc tcgcacggca agatggtgat ctatccgatc cgtgacgccg
     3601 atactgacgg cctgcagctc atcaattggg tcgccgaaat cgagacgccc aattatcgca
     3661 agcgggactg gaacagggcc ggctcgatcg aggatttcat cggtgcattc gccgactggc
     3721 atttcgactg gctggacgtg ccagcctttg tccgcgccgc cgacctcgtg ctggagtttc
     3781 cgatggtcga ccaggacccg ctgccgcgct ggagctttgg ccgggtgacg ctgctcggcg
     3841 atgccgcaca tccgatggtg ccgcgcgggt ccaacggcgc cggccaggcg atcctcgacg
     3901 tgcgggcgct gaccacggcg ctccgcgagc acgccgatcc ggtcgcggcg cttgctgcct
     3961 atgaggcgca gcggctcgag gctacgacgc gcgtcgtgct gaccaaccgc accaatccgc
     4021 cggacgcgat cctgcgcgag gtgtacgagc gaacccgcga ccggccattc gctgcgattg
     4081 gcgatgtcat cagccacgag gaacttgtgg cgctgtccga aggctacaag cggatcgcgg
     4141 gttattcgaa ggatgccttg cgcgggtgac aaagggcggt aggctgaccg cacaaggcgg
     4201 tcggcgagaa ccgcgccagc caggggaagg ggacgatcat ggccaatacg caggcggaca
     4261 attcgccggc gatcgacctc gtcgagttca tcgaccgcca gccggtcggc ggattccaga
     4321 tccggctgct cctgctttgt gcggccgtgt tgttcctcga cggtttcgat acgcaggcca
     4381 tcggttatgt cgcgccggcg atcgccaagg aatggggact aaccaaaggt gcgcttggcc
     4441 cggtgttcag cgccggcctg ttcggcctca tgatcggcgc actggtgttc ggcccgcttg
     4501 cggaccatat cgggcgcaag aaaatcatca tcttctcgac ggccgctttc ggcgtcggag
     4561 cgctggtgac ggccttcgtc cacgacgtca gtgcgctcct cgtaatccgt ttcctgaccg
     4621 ggcttggttt gggcggtgcc atgccgaaca cggtcgccat gacctcggag ttcaacccgc
     4681 agcgccgccg cgccaccatg gtcatgatca tgttctgcgg cttttccgtc ggtgcggcgc
     4741 tcggcggtct gctcgcggcc gatctgattc cgcgcttcgg ctggcgcgcg gtgttcatcg
     4801 tcggcggcat agctccgctc ctgatgttgc cgatcctggt ctggcggctg ccggaatcgg
     4861 ttcgcttcct tgcaaccttc gggcacgcgc ccgaacgcgt cgcagaattg ctgaagcgga
     4921 tcagccccaa cacggcgttc gcgccggagg cgcgcttcgt cgtgcacgag acccatcttg
     4981 ccgggctgcc ggtcaagcac ctgtttcgcg agggacggag ccttgcgaca tccttgctgt
     5041 gggtggtgtt cttcatgagc ctgctcgata tctattttct ggcgaactgg ctgccgacgg
     5101 tgctcaacga tctcggcgcg tcggtctcgg aagccgtgct gatcggctcg ctgctgcaga
     5161 tcggtggcgt ag
//
LOCUS       DATLJT010001491         3809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3813456,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001491 DATLJT010000000
VERSION     DATLJT010001491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813456"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1091
                     /locus_tag="VM910_34160"
     CDS             462..1091
                     /locus_tag="VM910_34160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI67590.1"
                     /translation="MKRRSAFAAALAVFLCASAHAEDKAVSPHELKAKTDYCKTCHGL
                     SGQGFRGSFPMPRLAGQSPEYLQNQLQAFIDRRRTNPVMFNVAHVLNPSMQKALADHF
                     RDLNPRPLGGAPRELVGEGKRIYQEGVPAANVPPCASCHGPEAKGADAFPRLAGQLND
                     YIIKKLTNWDKERGQQKDQPDNSAIMQPIAHDLTEAQIRAVAAYVSYLE"
     gene            complement(1125..3419)
                     /locus_tag="VM910_34165"
     CDS             complement(1125..3419)
                     /locus_tag="VM910_34165"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVI67591.1"
                     /translation="MFERALRWALVFIAVLGLAAGGIAYARHRGDLADLSFTLATVPV
                     VVALAISILRDFLAGRLGVDAIALVSMSAAIALGQPLAGAVVALMYSGGNVLEDIAVA
                     RAEHDLRSLVDRAPREAHRHTGDRFEDVPISDVSVGDRLMVRAGEIVPVDGVVSSAAA
                     TIDESALTGEPIPVIRTRGAAVLSGSLNAGETFEMSVAAEAGESTYAGIVRLVTAAQT
                     AKAPFVRLADRYALIFLPVTIAVATIAWLISGDLIRSLAVLVAATPCPLILAAPVAFI
                     AGVAQAARRGILVKGGGPLEALARAHTVLFDKTGTLTVGGARLLSVEVAPGETANDVL
                     MFGASLEQASHHVLANAIVAASAARGLKLKVPEQVRETMGSGLVGVIDGRQVSAGARD
                     KILPPGHLAPWASRAIRRASWRSALVVFVAIEGKPIGALLLADELRSDTPRAIRLLRD
                     AGIARMVMVTGDRAAAAQAIGAALDLDAVLADRVPSDKVDAVRSEQQLHPTIMVGDGI
                     NDAPALASADVGIALGARGASASSEAADVVILADRLDRVGEAILIAQRARRIAVESIV
                     AGMGLSLAAMAAATLGWLAPVPAAIVQEVIDVAVILNALRALAPAYAHAGKSMTVEDG
                     DRLRRGHQALIAHLDRLRSIVDALDDVAPEKGATLIAEANRLVQEQVVVHERDDEGSI
                     YPRVVKLLRESHSLSAMSRAHREILHLARLLARVADDLPSEKVDRYFIRDAQRIIEAI
                     ETLVRMHTAQENDIYEAVAARTAA"
     gene            complement(3543..>3809)
                     /locus_tag="VM910_34170"
     CDS             complement(3543..>3809)
                     /locus_tag="VM910_34170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67592.1"
                     /translation="AAATGFTRSLAREVGKLGITVNAIAPGFIATELTQSLSDEARQR
                     IAGRSALRRLPEADDVARMVEFLLGEGGRNITGSVLTVDAGNTA"
BASE COUNT          746 a         1259 c         1184 g          620 t
ORIGIN      
        1 aggcttgggc tggcagcgga aagcctatcg ccagataggc tacgagagcc gcaacgattt
       61 cgaacgcagc aaggagtctg ctggttctca agcttcgaac aaaacgctcg cctcgcattg
      121 cgcaccaatc ccgccgcggc gatcgaggca aatacgaatc tcgatgtgtc gccgcttgcg
      181 aagcactagg cccggactgg catgcgtcgc caagtgcgtg cagcaggaat tgcttcgatt
      241 tcggagaaag cgaggcattt aagcaacatt agttgcattt gcaactaatt ctcactcggc
      301 aaaaaccggg gtttcgccgt tcggtgattc gcgcaatcct gcatcagatg tgcgtcgtcg
      361 catcgcgtct ttgacctgga tcaaatttgt caaaaaaaga tcagctggat cggcatgcga
      421 gacaaagcgt atttagattc gggctaacgg gtggattgac catgaagcga cgatcggctt
      481 ttgcggccgc attggctgtt ttcctgtgtg cgtcggcgca cgcggaggac aaggccgtct
      541 cgccacacga gctcaaggcc aagactgatt attgcaagac ctgccacggc ctttcggggc
      601 aaggctttcg cggatcattt ccgatgccgc ggctggctgg acagtcgccg gagtatctgc
      661 agaaccagtt gcaggcgttc atcgaccgca ggcgcacgaa cccggtcatg ttcaacgtgg
      721 cgcacgtcct taatccatcg atgcaaaagg cgctcgccga tcatttcaga gacctcaatc
      781 ccaggccgct gggcggagct ccaagggagc tcgtgggcga aggcaagagg atttatcaag
      841 aaggcgtgcc ggccgccaac gttcctccct gcgcctcctg tcacggaccc gaggccaagg
      901 gagccgatgc cttcccgcgc ctcgctggcc aactcaacga ctacatcatc aagaagctga
      961 cgaactggga caaggaacga ggacagcaaa aggatcagcc ggataactcg gcgatcatgc
     1021 agccaatcgc ccatgatctg actgaagcgc aaatcagggc tgtcgctgcc tatgtcagtt
     1081 atctggaata aggtcctgta cacgttggcg cgaaggaagg ccggtcaggc cgccgtccgc
     1141 gccgcaaccg cctcatagat gtcgttctcc tgcgcggtgt gcatccgcac cagagtctcg
     1201 atcgcctcga tgatccgctg tgcgtcgcgg atgaaatagc gatcgacctt ctccgacggc
     1261 aggtcgtccg ccacgcgcgc cagcaggcgg gcgagatgca ggatctcgcg atgcgcgcgg
     1321 ctcattgccg agaggctatg gctctcgcgc agcagcttca cgacgcgcgg atagatcgag
     1381 ccttcgtcgt cgcgctcatg cacgacaacc tgctcctgca ccagcctgtt cgcctccgcg
     1441 atcagcgttg cccccttctc cggcgccaca tcgtcaagcg cgtcgacaat cgatcgcagc
     1501 cggtcaagat gagcgatcag cgcctgatgg ccgcgccgca accggtcgcc atcctccacc
     1561 gtcatgctct tgccggcatg agcataggcc ggcgcaagcg cacgcagcgc gttgaggatc
     1621 acggcgacat cgatcacttc ctgcacgatc gcggccggta ccggcgcgag ccagccgagg
     1681 gtcgcggccg ccatcgctgc gagcgaaagt cccatccccg caacgatgct ttcgaccgca
     1741 atccgccgcg cccgctgcgc aatcaggatc gcctccccca cccggtcgag ccggtccgcc
     1801 aggatcacca catcggccgc ttccgaactg gcgctggcgc cgcgggcgcc gagcgcgatc
     1861 ccgacatcgg cagacgccaa tgccggcgca tcgttgatgc cgtcgccgac catgatggtc
     1921 ggatgcaact gttgctcgga acgcaccgcg tcgaccttgt ccgagggaac gcggtcggcg
     1981 agcacggcgt cgagatcgag cgctgcgccg attgcctgag ccgcggcggc gcggtcgcct
     2041 gtgaccatca ccatgcgtgc gatgccggcg tcgcgcagca gacggatcgc gcgcggcgta
     2101 tcggaacgaa gctcgtcggc aagcagcagg gcgccgatcg gcttgccctc gatggcgacg
     2161 aacacgacca gcgccgagcg ccacgacgcc cgccggatcg cacgcgacgc ccacggcgcg
     2221 aggtggcctg gcggaaggat cttgtcgcgg gcgcctgcgc tgacctgccg cccgtcgatg
     2281 acgcccacga gccccgagcc catggtctcc ctgacctgct cgggcacttt gagcttgagg
     2341 ccgcgcgcag cgctggcggc gacgatcgcg ttggcgagca catggtgcga ggcctgttcg
     2401 agcgatgcgc cgaacatcag cacgtcgttg gccgtctcgc cgggcgcaac ctcgacggaa
     2461 agcagccgtg cgccgccgac cgtgagcgtg ccggtcttgt cgaacagcac ggtatgggcg
     2521 cgcgccagag cttccagcgg accgccgccc ttgaccagga tgccgcgcct tgccgcctgc
     2581 gcaacaccgg cgatgaaggc cacgggcgcg gccagaatca gcgggcacgg cgtcgccgcg
     2641 accaaaacgg cgaggctgcg gatcaagtcg cccgagatca gccaggcaat cgtggcaacg
     2701 gcgatggtca ccggcaggaa aatcagggca tagcggtcgg ccagccggac gaagggcgct
     2761 tttgcagtct gcgctgcagt caccaggcgc acgatgccgg cataggtgct ctcgcccgct
     2821 tccgccgcca cggacatttc aaaggtctcc ccggcattca gcgaaccgga gagcacggca
     2881 gcaccgcgcg tcctgatcac cggaattggc tcaccggtca gcgccgattc gtcgatggtc
     2941 gccgcggcgg atgaaacgac gccatcgacg ggcacgatct caccggctcg caccatgagg
     3001 cgatccccga ctgagacgtc cgatatcggc acatcctcga agcgatcgcc ggtgtggcga
     3061 tgcgcttcgc gcggagcgcg gtcgaccagc gaccgcaaat catgttcggc ccgcgcgacc
     3121 gcgatgtcct cgagcacatt gccgcccgaa tacatcaatg cgaccacggc gcccgccaat
     3181 ggctggccaa gtgcgatcgc tgcactcatt gataccagcg cgatcgcatc cacgccgagg
     3241 cgtccggcca ggaaatcgcg gagtatcgaa atcgcaagcg cgacgacgac gggaacggtc
     3301 gcaagcgtga agctgagatc ggcaagatcg ccacggtgcc gcgcataggc gataccgccc
     3361 gcagccaggc ccagcactgc aataaagacg agcgcccacc gcagcgcgcg ttcgaacatc
     3421 gatttatcct gctttttatc tacgccggtg aaacttgggc gaaaagccgc ccgcgacgtt
     3481 tgaggaagat caaaatcggt aggcggtctg cgacctgcga acaggacgca aatgcagcat
     3541 cgtcacgcgg tattgccggc gtctaccgtc agaacgctgc ccgtgatatt gcggccgcct
     3601 tcgccgagaa gaaattccac catgcgcgcg acgtcatcgg cttccggcag ccgccgcagc
     3661 gcgctccgac cggcgatgcg ctggcgcgct tcgtccgaga ggctttgcgt caattcggtc
     3721 gcaatgaaac ccggcgcgat cgcattcacg gtgatgccga gcttgccgac ctcccttgcg
     3781 agcgagcgcg tgaaaccggt cgcagccgc
//
LOCUS       DATLJT010001492         1565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1054217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001492 DATLJT010000000
VERSION     DATLJT010001492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1404
                     /locus_tag="VM910_34175"
     CDS             112..1404
                     /locus_tag="VM910_34175"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVI67593.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLV
                     INGETYKLALLPSGVLRPSKLAVIGNGVVFDPQAFLDEIAKLKSQGVAVSPDNLRIAE
                     NVTLILPLHRELDALRESSNAVTAIGTTRRGIGPAYEDKVGRRAIRLMDLADLDTLPH
                     KIDRLLAHHNALRRGLNLKEIDGKGILRELTELAPKLLPYAETVWRLLDLRRREGKRI
                     LFEGAQGALLDVDHGTYPYVTSSNTVAAQAATGTGMGPGGVGYVLGICKAYTTRVGQG
                     PFPTELNNEIGEEIGRRGREFGVNTGRKRRCGWFDAMLVRQTVRTCGINGLALTKLDI
                     LDGFDTIEVCIGYRLDGKEIDHLPAGEGAQARVVPIYEKIEGWKQPTANARSWAELPA
                     QAIKYVRRVEELVGCPIALLSTSPEREDTILVQNPFEA"
BASE COUNT          289 a          480 c          520 g          276 t
ORIGIN      
        1 tttcggccgg gtgtatgatc gggacaaggc ctgtgtcgga cgtgttttgg ggcgcgccgg
       61 ctttccgaac cgatagctcg agtgacgagg tttcagggga ctagatcgcg aatggccaac
      121 gttgtcgtcg tcggcgccca gtggggcgac gaagggaagg gcaagatcgt cgattggttg
      181 tcggagcagg ccgatatcgt tgtgcgcttt cagggcggcc acaatgccgg ccacacgctc
      241 gtcatcaacg gcgaaaccta caagcttgcc ctgctgccat caggcgtgct gcggccctcg
      301 aaactggccg tgatcggcaa cggcgtggtg ttcgatccgc aggcgttcct cgacgagatc
      361 gcgaagctga agtcccaggg cgtcgccgta agcccggaca atcttcgcat cgcggagaac
      421 gtgactctga tcctgccgct gcatcgcgaa ctcgatgcct tgcgcgaatc ctccaatgcc
      481 gtcacagcga tcggcaccac gcggcgcggc atcggcccgg cctatgagga caaggtcggc
      541 cgccgcgcca tccgtctgat ggatcttgcg gacctcgata cgctgccgca caagatcgac
      601 cggctgttgg ctcatcacaa cgcgttgcgg cgcgggctca atctcaagga gatcgacggc
      661 aagggcattt tgcgcgagct gactgaactg gcgccaaagc tcctgcctta tgccgaaacg
      721 gtgtggcgtc tgctcgactt gaggcggcgc gagggcaagc gcatcctgtt cgagggagcc
      781 cagggcgcgc tgctcgatgt cgaccacggc acttacccct acgtcacatc ctccaacacg
      841 gtggcggcgc aggcggcgac cggaaccggc atgggaccgg gcggggtcgg ctatgtgctc
      901 ggcatttgca aggcctacac gacccgggtc ggccaggggc cgttcccgac cgagcttaac
      961 aacgaaatcg gcgaagagat cggccgcagg ggccgggagt tcggcgtcaa caccgggcgc
     1021 aagcgacgct gcggctggtt cgacgcgatg ctggtgcgcc agacggtcag gacctgcggc
     1081 atcaacgggc tggcgctgac caagctcgat attctcgacg gcttcgacac gatcgaggtc
     1141 tgcatcggct accggctgga cggcaaggag atcgaccatc tgccggcagg cgagggggct
     1201 caggcccggg tggtgcccat ctatgagaaa atcgagggct ggaagcagcc caccgccaat
     1261 gcccgctcct gggcggaatt accggcccag gccatcaaat atgttcgccg cgtcgaggaa
     1321 ctggtgggtt gcccaattgc cttgctttcc accagcccgg aacgcgagga tactattctg
     1381 gtccagaatc ccttcgaggc ttaacgagat attaccggga cgtgcgcata gctgagttga
     1441 tatggccgac tactacccgc tgattgcccg cgctatcgct ggattggacc ccaatgctcc
     1501 cggcgagagc aggcgtgcgc tctacgagcg ggcgcgcgcg gcgttgattg cgcagctccg
     1561 cagcg
//
LOCUS       DATLJT010001493         1937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2893358,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001493 DATLJT010000000
VERSION     DATLJT010001493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893358"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..650
                     /gene="maiA"
                     /locus_tag="VM910_34180"
     CDS             18..650
                     /gene="maiA"
                     /locus_tag="VM910_34180"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVI67594.1"
                     /translation="MKLHGYFRSSASYRVRIALNLKGLRVDHLPHHLRKGEQFAPAYL
                     AINPQGLVPTLENDAGAILSQSLAIIEWLDETHPTPPLLPKEPLRRAKVRAFAQVLAC
                     DTHPVQNLKVLARLRELGLPEEKVTAWAGWANREGLSACDKLIATEAGPFCFGDAPTM
                     ADLCLVPQLANARRFGVDVSVFPRLLQAEAAAKALPAFADAAPDKQPDAE"
     gene            640..1149
                     /locus_tag="VM910_34185"
     CDS             640..1149
                     /locus_tag="VM910_34185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI67595.1"
                     /translation="MPSSKTPITMDAVYTAPGYLFRRMQQIAVAIFVEECRAFDLTPV
                     QYAALIAIHTHPGIDATRLSAVIAFDRSTLGSVIERLEAKELIERKPSPDDRRVKLLH
                     LTKAGAALLRDIKPSVDRAQVRMLQPLKPADRKTLMALLTQLVDLNNEASRVPLRAEE
                     VLERLGRLG"
     gene            complement(1211..>1937)
                     /locus_tag="VM910_34190"
     CDS             complement(1211..>1937)
                     /locus_tag="VM910_34190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVI67596.1"
                     /translation="ADGASNADFSSVRLSVSAAEVLSSEVFNGWKALTGLEIVEGLGS
                     TEVLHIYLCNHEDRKKPGAAGLRVPGYEIALRDKDGREVGDNEEGILWVRGDSSTPLY
                     WNRPDKTAELVREEGWIYTGDRFVRDSDGFHFFRGRADDLVKISGQWVYPLEVELCLI
                     EHPDVRECAVFAAELPDRRMTLKAVVVMNKGGYDERDATKRLQDYVKEKLLPYKYPRE
                     IKFIGELPKTGTGKIDRQAIMRM"
BASE COUNT          342 a          638 c          598 g          359 t
ORIGIN      
        1 tgcaggggag gtgtgcgatg aagctgcacg gctatttccg cagcagcgcg tcctaccgcg
       61 tcagaatagc cttgaacctg aagggtttgc gcgtggacca tctgccgcac catctgcgca
      121 agggcgagca gttcgcgccg gcctatctcg cgatcaaccc gcagggtctg gtgccgacgc
      181 tggagaacga tgccggggcg atcctcagcc agtcgctcgc catcatcgaa tggctggacg
      241 agacccatcc cacgccgccg ctgttgccga aagagccgct gcgccgcgcc aaggtgcgcg
      301 cctttgcgca agtgctcgcc tgcgacactc atcccgttca gaacctcaaa gttctggcgc
      361 ggctgcgcga gctcggcctg cccgaggaaa aggtcaccgc atgggccgga tgggccaatc
      421 gcgaagggct ttcggcctgc gacaaactga tcgcaactga ggccggcccg ttctgcttcg
      481 gcgacgcccc gaccatggcc gatctctgcc tggtaccgca gcttgcgaat gcccggcgtt
      541 tcggcgtcga cgtttcggtt ttcccgcggc tgttgcaggc ggaggccgcg gccaaagcct
      601 tgccggcatt tgccgacgcc gccccggaca agcaacccga tgccgagtag caagacgccg
      661 atcacgatgg acgcggtcta taccgcgcca ggctatctgt tccgccgcat gcagcagatc
      721 gcggtcgcga tcttcgtcga ggagtgcagg gcgttcgacc tgacgccggt gcaatatgcg
      781 gcgctgatcg cgatccatac ccatcccggc atcgacgcga cgcggctctc cgccgtgatc
      841 gcattcgacc gctcgacgct tggcagtgtg atcgagcggc tcgaagcaaa ggagctgatc
      901 gagcgcaagc cgtcgccgga cgacaggcgg gtcaagcttc tgcatctgac gaaggccggc
      961 gcggcgctgc tccgcgatat caaaccctcg gtcgatcggg cgcaagtgcg catgctgcaa
     1021 cccctgaagc ctgccgaccg caagacattg atggcgctgc tgacgcagct cgtcgatctc
     1081 aacaacgaag cctcgcgcgt gccgctgcgc gcagaagagg tgttggagcg gctggggagg
     1141 ttgggctgag ggcgagcctg gctgagattc tgaattgttt cgtttcgctt cgtaatgacg
     1201 ggcaagctca ctacatccgc atgatcgcct ggcggtcgat cttgccggtc ccggtcttcg
     1261 gcagttcgcc gatgaacttg atctcgcgcg gatatttgta aggcagaagc ttctccttga
     1321 cgtaatcctg cagtcgcttc gtcgcatcgc gctcgtcgta tccgcccttg ttcatcacca
     1381 ccacggcctt cagcgtcatg cggcggtccg gcaattcggc ggcgaacacg gcgcattccc
     1441 tgacgtcggg gtgctcgata aggcaaagtt cgacctccag cggatagacc cactggccgg
     1501 agatcttgac caggtcatcg gcgcggccgc gaaagaagtg aaagccgtcg ctgtcgcgca
     1561 caaaacgatc gccggtgtag atccagcctt cttcgcggac cagttccgcg gtcttgtccg
     1621 gccggttcca gtaaagcggc gtcgaggaat cgccgcgcac ccataaaatg ccctcctcgt
     1681 tgtcgccgac ctcgcggccg tccttgtccc gcagcgcaat ctcatatccg ggtacgcgaa
     1741 gcccggccgc gcccggcttc ttcctgtcct cgtggttgca cagatagatg tgcagcacct
     1801 cggtcgagcc cagcccttcg acgatctcga gaccggtcag tgctttccag ccgttgaaga
     1861 cttcgctcga cagcacctcg gccgcggaga ccgaaagacg caccgaggaa aagtccgcat
     1921 tgctcgcgcc gtcggcc
//
LOCUS       DATLJT010001494         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2894093,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001494 DATLJT010000000
VERSION     DATLJT010001494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894093"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VM910_34195"
     CDS             complement(<1..609)
                     /locus_tag="VM910_34195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HVI67597.1"
                     /translation="MPLRIEDYGLIGDCETAALVGRDGSIDWLCWPAFDSDACFAAIL
                     GDARNGRWLIAPAAKVTKSSRHYWDNTLILETRFETADGIVALVDFMPPRGTASDVVR
                     LVRGVKGRVKMRMELVIRFGLGADIPWVRRTEDRSAVLAICGHDMTVLRTPVETRGEN
                     MTTVADFVVSEGETVPFVLTYGPSHLAVPEPINPAVALDETEA"
     gene            complement(632..>2248)
                     /gene="malQ"
                     /locus_tag="VM910_34200"
     CDS             complement(632..>2248)
                     /gene="malQ"
                     /locus_tag="VM910_34200"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVI67598.1"
                     /translation="AVSEEVPLVVAPARAFAGDFDRGWLLAVQLYGIRSARNWGIGDF
                     TDLKGLIELAAHLGADGVGLNPLHVLFDDRPGDCSPYSPNSRLFLNPLYIDVEQIPEY
                     QGGPDAEALARLQRAVTVDYIAVAEQKWRAMRSAFAAFMTGANAARRQDFEEFRTEQG
                     SLLAHFACFEVLRHRFDKPWWEWPEEWQRPDDAKCADLRAGHDKREIEFVEFVQWTAD
                     RQLRSCKALADSLGMKVGLYLDVAVGVQSNGFDAWNEQEAISRHLAVGAPPDPLNVLG
                     QNWGLAGFNAAGLEIKHFAPFRDMLRASMRYAGAIRLDHVLGLKRLYLVPHGFPAKNG
                     AYVQMPLEALFAVTALESVARGCVVIGEDLGTVPEGFREQMADWGIWSYQVMMFGRDD
                     EARFFGIEHYWPNALITFNTHDLSTYAGWRSFSDLKMKRSLGIDPGETDDARWHALGM
                     LDEVLRRYGIHHHDLYSVAGFLARTKSRLMALSMEDLLGVVDQPNIPGTIDEHPNWRQ
                     RLPVALDAIGATIDVGALRAATGERSRLED"
BASE COUNT          431 a          741 c          702 g          374 t
ORIGIN      
        1 cgcctcggtc tcgtcgagcg ccactgccgg attgatcggc tccgggaccg caagatgcga
       61 cggaccgtag gtgagcacga agggaacggt ttcaccttcg cttacgacga aatcggccac
      121 cgtggtcata ttctcgccgc gggtctcgac cggcgtgcgc agcaccgtca tgtcgtggcc
      181 gcagatcgcg agcaccgccg agcggtcttc ggttcgccgc acccatggaa tgtccgcgcc
      241 gagaccgaat cggatgacga gctccattcg cattttgacg cggcccttga cgccgcgcac
      301 caggcggacg acgtcggaag ccgtgccgcg cggcggcatg aaatcgacca gcgcgacgat
      361 gccgtcggcg gtttcgaacc gggtttccag gatcagcgta ttgtcccaat agtggcgtga
      421 ggatttcgtc accttcgcgg ccggcgcgat caaccagcgg ccgtttcggg catcgccgag
      481 gatcgccgca aaacaggcgt cggaatcgaa ggccggccag caaagccagt cgatcgaccc
      541 gtcacggccg accagggctg cagtctcgca gtcgccgatc aggccatagt cttcgatccg
      601 caagggcaat gttcatcacc cgctcaaatt ttcagtcctc aaggcgagac cgctccccgg
      661 tcgcagcccg cagcgcgccg acgtcgatcg tggcgccgat tgcgtcgagc gcaaccggca
      721 ggcgctgccg ccagttcgga tgctcgtcga tggtgccggg aatgttgggt tgatcgacca
      781 caccgagcag atcctccatt gataacgcca tcagacgcga tttggtccgc gccaggaagc
      841 cggcgacaga ataaagatcg tgatgatgga tgccgtaacg gcgcaacacc tcgtccaaca
      901 tgccgagcgc gtgccagcgc gcatcgtcgg tctcgccggg gtcgatgccg agcgagcgct
      961 tcatcttcag gtcggagaac gagcgccagc cggcgtaggt cgaaagatcg tgcgtgttga
     1021 acgtgatcag cgcattcggc caatagtgct cgatgccgaa aaaccgagcc tcatcgtcgc
     1081 gcccgaacat catcacctgg tacgaccaga tgccccaatc ggccatctgc tcgcgaaagc
     1141 cttccggcac cgtgccgaga tcctcgccga tcacgacgca accgcgcgcg acgctttcca
     1201 gcgccgttac cgcaaacagc gcttccagcg gcatctgcac ataggcgccg ttcttggcgg
     1261 ggaagccgtg cggaacgagg taaagccgtt tcaggcccag cacgtgatcg agccggatcg
     1321 cgccggcata acgcatcgat gcgcgcagca tgtcgcgaaa cggcgcaaag tgcttgatct
     1381 cgaggcctgc agcattgaag ccggcgagcc cccagttctg ccccagcacg ttcagcgggt
     1441 cgggcggcgc gccgaccgca agatggcgcg agatcgcctc ctgttcgttc cacgcatcga
     1501 agccgttgga ctgcacgccg acggcaacgt cgagataaag cccgaccttc atgccgagac
     1561 tgtcagcgag cgccttgcag gaccgcaatt gccgatcggc ggtccattgc acgaattcga
     1621 cgaactcgat ctcgcgcttg tcgtggcctg cgcgcaaatc cgcgcatttg gcatcgtcgg
     1681 gccgctgcca ttcttccggc cattcccacc atggcttgtc gaagcgatgc cgcagcactt
     1741 cgaaacaggc gaagtgggcg agcagcgatc cctgctctgt gcgaaactcc tcgaaatcct
     1801 ggcggcgggc cgcgttcgcg cctgtcatga acgccgcaaa cgccgatcgc atcgcccgcc
     1861 atttctgctc ggcgacggcg atataatcga cggtgacggc gcgttgcagc cgcgcgaggg
     1921 cctcggcgtc cggcccgccc tgatattccg ggatttgctc gacgtcgatg taaagcggat
     1981 tgaggaaaag ccggctgttc ggcgaatagg gactgcaatc gccgggccgg tcgtcgaaca
     2041 ggacgtgcag cggattgagg ccgacgccgt cggcgccgag atgcgcggca agctcgatca
     2101 aacctttcag atcggtgaaa tcgccgatcc cccaattgcg cgcggagcgg atgccataga
     2161 gctgcaccgc cagaagccag ccgcggtcga aatcgccggc gaaggcgcgc gctggcgcga
     2221 cgaccagcgg cacctcttcg ctcaccgc
//
LOCUS       DATLJT010001495         2377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1055181,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001495 DATLJT010000000
VERSION     DATLJT010001495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055181"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..109)
                     /locus_tag="VM910_34205"
                     /pseudo
     CDS             complement(1..109)
                     /locus_tag="VM910_34205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            233..1507
                     /locus_tag="VM910_34210"
     CDS             233..1507
                     /locus_tag="VM910_34210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HVI67599.1"
                     /translation="MTRDSTAAIRRSNGPVDEAFEDVRKSFERFCLAAGIETLGTMME
                     ADVEAACGARHARTPARRAHRWGRTQGWLGFHGGKIAVERPRVRDLGGREVAIPSWET
                     AAAEDWLGRWAMNQMLINVSTRKFARSVRLPEGDVAAGPGTGLSKSAASRHFVALSAD
                     KLEEFMAADLSRLDLLAVQIDGLHVSDGLVLVAAIGIDGEGDKHPLALVEGATENTAT
                     VQALIDNLIERGLDPTAPRLFIVDGAKALSKAIRASFGKDVAIQRCQIHKSRNIMDRL
                     PKDLHASTRRVLRQAWELDDAAKAETLIRNLARSLDRDRPGVAASILEGLDEILTVAR
                     LKLPKELRRSLACTNIAENMMGTIRRVTRNVKRWQDGRMALRWVAAGMMEATKGFRRL
                     KAYKQLPALRAALQVHRELLTEKPVVLPNRAA"
     gene            2068..2202
                     /locus_tag="VM910_34215"
                     /pseudo
     CDS             2068..2202
                     /locus_tag="VM910_34215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
BASE COUNT          492 a          695 c          763 g          427 t
ORIGIN      
        1 atttggaata atcaaggaat gccgataccc gagcgcttag caggcgcctg aatgggtaga
       61 gcgtccacag ggcaatgtcg ggtccgtcga tttctcccca gtgcaccaaa actaaacgtg
      121 tacggctttt caccggggga tgtcccgggg gctgttgaaa atgggagggc ggcgttgctt
      181 gccttgagcc gttaggctcg gggtgctgat tccacgaaag gaaagcaacg ccatgacgag
      241 agatagcaca gcggcaatac ggcggtcgaa cgggcctgtg gacgaggcct ttgaggatgt
      301 ccgcaagagc ttcgagcggt tctgccttgc ggcaggaata gagacgctcg gcacgatgat
      361 ggaagccgat gttgaagcgg cctgcggcgc gcgccatgcc cggacgccgg cacgtcgggc
      421 tcatcgatgg gggcggacgc aaggttggtt gggtttccac ggcggcaaga tcgcggtcga
      481 acggccgcgg gtgcgcgacc tgggcggccg tgaggtcgcg ataccgagtt gggagaccgc
      541 cgcggccgaa gactggctcg gtcgctgggc gatgaaccag atgctgatca acgtctcgac
      601 acgcaaattc gcccgttcgg tgcggctccc tgagggcgac gtggcggccg gccccggaac
      661 gggcctgtcg aaatcggcgg cgtcgcggca cttcgtggcc ctgtcggccg acaagctcga
      721 agagttcatg gccgccgatc tctcgaggct tgatctgctc gccgtccaga tcgacgggct
      781 tcatgtcagc gacggtctgg tgctggtggc ggcgatcggc atcgatggtg agggcgacaa
      841 acatccgctg gcgctcgtcg agggcgcgac cgagaacacg gcgactgtcc aggcgctcat
      901 cgacaatctc atcgagcgcg ggctcgaccc gacggcgccg aggctcttca tcgtggatgg
      961 tgcgaaggcc ctgtcgaaag caatccgggc gagcttcggc aaggacgtgg cgatccagcg
     1021 ctgccaaatc cacaagtcgc gcaacatcat ggatcgcttg ccgaaggacc ttcatgcctc
     1081 gacccgccgc gtgctgcgtc aggcttggga actcgatgat gccgcgaagg cagagacgct
     1141 catccgcaac cttgcccgga gcctcgatcg ggaccggcca ggcgttgccg cgagcattct
     1201 cgaggggctc gacgaaatcc tcactgtcgc gcggctcaag ctgccgaaag agctgcgccg
     1261 ctcgctcgcc tgtaccaaca tcgccgagaa catgatgggc accatccgcc gcgtcacccg
     1321 caatgtgaag cgctggcagg acggcaggat ggcgctcaga tgggtcgctg ccggcatgat
     1381 ggaggccacc aaaggcttcc gaaggctcaa agcctacaag caacttcccg ctcttcgagc
     1441 cgcgcttcaa gtccatcggg agcttctcac cgaaaagcct gttgtcctgc caaacagggc
     1501 agcctaacct tcaactcggc agcgccggcc cgctcaagtt caacaccaga cggggcatcc
     1561 ccgcgcgcta tcgcgcagcg atttagtgca ttgtccctgg gcagacattc gatgagcggc
     1621 agatctcatg gcgacaagcg tagcttgaac acgggccggc gtgttgtgtc gcgctgcgct
     1681 caatcgtgag attcgggcga attccgcgaa ttcattgcac gaaatcatga gacgactttc
     1741 gacttccgtc tcataattgc agcaattgtg agacggtacg aaggatatag atagggcctc
     1801 tatcgagccc ttagctcgac accggacagc tagcgagcgg cgtctttgcg cctgcaatcc
     1861 ttcccccaat cgacggcata gtcggagccg ggctaagtcc gttcatgagg cgaatcgtag
     1921 ttcaacgtag ggagcccgga gtctcgcgtt aagtgcaata gaccaagcga aaatctcacg
     1981 attgaacgca atgtccaaaa ttaagtgcaa cgataactaa ttgaaagagc acaatctggc
     2041 gtctcacgat aaagcgcagc gtgacgctcc cagcgggaga tcgaacgcgt ggtgcgcgag
     2101 ggaaaggtga aagggtcgac cttgcgcctg aagatgacgc tgacatccgc ggatgcgacc
     2161 tcgctcaatc atgtggtcga gcagacaatc gagctgaaat gattccgacg tggcgcccct
     2221 caagctcgat gccgggaaag tgacggctgg gggaccacct tgcgttcccg ctcggtgcga
     2281 aacaggcttt cggcgccctt gcggcgctcc tcctcggtca gcatcggccg ctgtgaagta
     2341 gtggcggcca ggtctataag cagcgcagcg gattatt
//
LOCUS       DATLJT010001496         8228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1515901,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001496 DATLJT010000000
VERSION     DATLJT010001496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515901"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..831)
                     /locus_tag="VM910_34220"
     CDS             complement(58..831)
                     /locus_tag="VM910_34220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HVI67600.1"
                     /translation="MTTDEDLRYDVSDRIAEISLARAPVNALSLSLLEQLIAALRRAA
                     ADENVRAVVLTSAVPRRFSAGLDLRLMLGKSATDVRRLLDKLYVELADVQYNLGKPSI
                     AAVGGSARGGGMTLAISCDVLVAGESATFGYPEIDLGLVPAIHFVHLPRIVGRHRAFE
                     LLFSGRTFGAEEAFALGLVSRIVPDERVRDEARAMARVFATKPQTAMRLGRAAFVRVN
                     DLDYRRGIGEAVENFCNVAATADAQEGLRAFVEKRPPNW"
     gene            complement(882..1847)
                     /locus_tag="VM910_34225"
     CDS             complement(882..1847)
                     /locus_tag="VM910_34225"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HVI67601.1"
                     /translation="MSAAVLEVADLKKHFPVKRGIIRRTVGQVYAVDGVSFEVREGET
                     LGLVGESGCGKSTVARTVLRLLEPTSGTIKLGGRDITRLSKAELRPHRRGMQMIFQDP
                     YSSLNPRMTAGDIVGEPLEVHELAGRRQRTDHVAALFAQVGLSAAQMKSFPHEFSGGQ
                     RQRISIARALALNPKLIVADEPVSALDVSIQAQVINLMMDLQREKKLSYLFIAHGLAV
                     VEHISHRVAVMYLGKIVEYAEKKTLFLDPLHPYTEALLAAVPIPNPKLRRAKRPLGGD
                     VPSPVNPPPGCPFHTRCGYAQERCRREVPQLREVAPGHWVSCHLR"
     gene            complement(1844..2914)
                     /locus_tag="VM910_34230"
     CDS             complement(1844..2914)
                     /locus_tag="VM910_34230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67602.1"
                     /translation="MDRSGVNKAVAERGARPEGRPERPILDVAGLRTYFFTRLGIIKA
                     VDDLSFTLRPRETLAIVGESGCGKSMTALSVMRLIPDPPGRIVGGSIELDGRDLLELD
                     EAQMRAVRGNDISMIFQEPMTSLNPVLTIGAQISETARLHQDLTKAAAWEKAVEMLRR
                     VRIPEPAQRANEYPHQLSGGMRQRAMIAMALACNPKVLIADEPTTALDVTIQAQILDL
                     MLALQQEFGTAIILITHDLAVVAETSQWVIVMYAGKKVEEADVDALFEAPLHPYTLGL
                     LASIPRLTSMGGGPAGSAGRLKEIPGVVPPLYRLPEGCAFAPRCPFADDKCRASYPPL
                     EQKRPRHWAACWHSDRLAGAAP"
     gene            complement(2916..3809)
                     /locus_tag="VM910_34235"
     CDS             complement(2916..3809)
                     /locus_tag="VM910_34235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67603.1"
                     /translation="MSTVDFDAELVKAGAGAHGRLARLRYLGWRNPLGAAGAVIMGLF
                     VFAAVFANVLTSYDPLSTNAALSLAPPSSDHWLGTDPFGRDVYARIIYGARISLAVGI
                     GATMLGSGLGIIFGLASGYLGGWVDLIVQRVMDILQALPLLVLALVMAAALGPSLQNT
                     IIAISIPLIPYVARVIRSNTLALRELPFVEAAKAVGMSELRIATRHILPNTLAPLIVL
                     ATAQLGSTILTEAALSFLGLGVPEPYPSWGRMLSQSAAEYVRTAPWLVVFPGIAISLA
                     VFGTNLLGDALRDILDPRLRS"
     gene            complement(3831..4781)
                     /locus_tag="VM910_34240"
     CDS             complement(3831..4781)
                     /locus_tag="VM910_34240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67604.1"
                     /translation="MGAYLVRRLFLMLLTLFGISVIIFALLRVMPGNIVDILYDAAGY
                     VNPREKAKIEAELGLDKPIVVQYVAWIGGLAQGDLGYSYVSERPALEEIVPRIPVTAK
                     LAGLALCFAVVIGVPLGVISAVHQNSGLDYLLRVLSLTGLSMPSFWLGLLILMAFVAY
                     FDTIPIYTAPPETWWDELLLLSVPAAAVGFRSSALILRLTRSSMLEVLRQDYIRTARA
                     KGASERSVNYHHALRNAILPVVTVIGIEAAFLMGGLIVTETVFNVPGVARYLVTALTQ
                     RDYPIVQNLVMFVATIVVLINFAVDMLYAVLDPRIKYAES"
     gene            complement(4939..6540)
                     /locus_tag="VM910_34245"
     CDS             complement(4939..6540)
                     /locus_tag="VM910_34245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67605.1"
                     /translation="MRGVHRAVAASSLLIAAVSFTAAAADEPKHGGILKIYHRDSPPS
                     ASIHEEATYSTVIPFMPIFNNLVMYKQDVAQNSFQSIVPDLASSWSWSEDKTELTFKL
                     RQGVKWHDGKLFTANDVKCTFDLLMGKSKDSLRKNPRQDWYKNVAEVKVNGEDEVAFH
                     LRRPQPALLALLASGYSPIYPCHVSARDMRVKPIGTGPFKFVEFKQNESIKITRNEDY
                     WKKDRPYLDGIDFTIIPNRSTAILAFVAGKFDMTFPTEVTIPLLKDVKSQAPQALCSL
                     VPINVSTNLIVNRDKPPFDNADLRRAMALTLDRKAFVDILSEGKDDIGGSMLPPPGGV
                     WGMPPEVLKTIPGYGPDIKANRAQARKLMEKLGYGPDKRLPVKVSTRNIAIYRDPAVI
                     LIDQLKDIYIDGELEVVETSNWHSKVTRKDYAVGLNLTGSSVDDPDQNFYENYGCGSE
                     RNYTDYCNKELEKQFDQQSAETDLEKRKQLVWEIDKKLQEDVARPIILHSRRATCWSP
                     RVKGVTIMTNSSYNGWRFEDVWLDH"
     gene            complement(6874..7404)
                     /locus_tag="VM910_34250"
     CDS             complement(6874..7404)
                     /locus_tag="VM910_34250"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HVI67606.1"
                     /translation="MNSALLQNLMAGAGSSSQFIGGLVVALLYVVIGLLSAIGSILIF
                     RRIFQGRWEQIFWASFLVVIAAFYLSFAAYFGASTHAWQTEAVGVAVFLVCAAIGLFS
                     RFAIAVGYVMHGLWDLSHCLSGASLAGLSITEIPLGYGLFCSTYDFTVACYLMVSDTA
                     WHEPGKLDLYFWRHRA"
     gene            complement(7488..>8228)
                     /locus_tag="VM910_34255"
     CDS             complement(7488..>8228)
                     /locus_tag="VM910_34255"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI67607.1"
                     /translation="EKGLAWEDKHLVLRAAEHQQPWYLKLNRRAVVPTLVHDGHVVPE
                     SNVILEYLEDVFPQPGLRPNDPYHRSKMRLWIKQLDEDVHDACAAILSFGIAFRHQYL
                     ERAEHGKAMLERIPNIFKRQRRRDVVENGTRSQHFVSAVQRMLQLLDEMEEALAEHRW
                     LACEEYSLADVAFTPYLARLEHLNIFAMVSDRNRVAEWYGRCKARPSFEAGIKKWENP
                     SYIELMSRRGAEHWPEVQSIMAGLKSAA"
BASE COUNT         1612 a         2550 c         2546 g         1520 t
ORIGIN      
        1 agtgccagac tagcacatca gcacgcgcgt gcacgctgaa gggccatttg ccgccaacta
       61 ccaatttggc gggcgttttt cgacgaacgc ccgcagtcct tcctgagcgt cggcggttgc
      121 agcgacgttg cagaaattct ccaccgcctc accaatgccg cgccggtaat cgagatcgtt
      181 gacccgcacg aaagcagcgc gccccagccg catggctgtt tgcggcttcg tcgcaaaaac
      241 acgggccatc gcccgtgctt cgtcccgcac ccgctcgtcc ggcacgatcc ggctgacgag
      301 gccgagagcg aaggcttctt cggcgccgaa agtgcggccg ctgaacaaga gctcgaaggc
      361 gcgatggcga ccgacaatgc ggggcaggtg cacaaaatga attgctggaa cgaggccgag
      421 atcgatttcg ggataaccaa acgtcgcgct ctccccggca accaacacgt cacaggagat
      481 ggccagcgtc atgccaccac ctcgcgcaga gccgccgacg gcggcgatcg acggcttgcc
      541 gagattgtat tgcacgtcgg ccaattccac gtacagcttg tcaagcaatc gccgcacgtc
      601 ggttgccgac ttgccgagca tcagacgcaa gtccaacccc gcgctgaatc gacgcggcac
      661 cgcgctcgtc agtacgacgg cgcgaacgtt ctcgtcggcg gcggcccgcc gcagcgccgc
      721 tataagttgc tcgagcaacg aaaggctcaa cgcattcacc ggcgcccgcg cgaggctgat
      781 ctccgcgatc cggtcggaaa cgtcgtaccg caagtcttca tccgtcgtca ctgccggcct
      841 cctgatgtag cgaaaagcgg tccgtgcggt aggtgctgga gctagcgaag atgacatgaa
      901 acccagtgtc cgggtgcgac ttcacgcaac tgcgggactt cccgacggca gcgctcctgc
      961 gcgtagccgc agcgcgtgtg gaatgggcag ccgggcggcg ggttcaccgg acttggcacg
     1021 tcgccgccaa gcgggcgctt ggcccgtctg agcttgggat tggggatcgg caccgcggca
     1081 agcaaggcct ccgtgtaggg atgcaatgga tcgaggaaca gcgtcttttt ctccgcatac
     1141 tcgacaatct tgccgagata catcaccgcg acgcggtggc tgatatgctc gaccaccgca
     1201 agtccatggg cgatgaacag gtatgagagt tttttctccc gctgcaggtc catcatcagg
     1261 ttgatcacct gcgcctggat cgacacgtcg agcgcggaaa cgggctcatc ggcgacgatc
     1321 agcttggggt tcagcgcaag cgcgcgcgca atcgaaatcc gctggcgctg accgccggag
     1381 aactcgtggg ggaagctctt catctgcgcg gccgagaggc cgacttgcgc gaacagcgcc
     1441 gcgacgtggt cggtcctttg ccttcggccg gcaagctcat gcacctcgag cggctccccc
     1501 acgatgtctc ctgccgtcat ccggggattg agcgatgaat acggatcctg gaagatcatc
     1561 tgcatcccgc gccgatgcgg gcgcaactcg gccttgctca gacgcgtgat gtcacggccg
     1621 ccgagcttga tggttccgct cgtcggctcg agcagccgca gcacggtacg tgccaccgtc
     1681 gacttgccac agccgctctc gccgaccagc cccagggttt cgccttcgcg gacttcgaag
     1741 ctgacgccat cgaccgcgta gacctgcccg acggtgcgac ggatgattcc tcgcttgacg
     1801 ggaaaatgct ttttgaggtc ggcgacctcg agcacggcgg cactcacggg gcagctccag
     1861 cgagccgatc cgagtgccag caggcggccc agtgtcttgg ccgtttttgc tcgagcggcg
     1921 gatagctcgc gcggcatttg tcgtcggcga atgggcagcg gggcgcgaag gcgcatccct
     1981 cgggaagacg gtagagcggc ggcacgacgc ccggaatctc tttgaggcgc cctgcgctgc
     2041 ccgcgggccc gcctcccatg ctggtcagac gtggaatcga agccagaagt ccgagcgtat
     2101 atggatgcag cggcgcctcg aacagtgcat cgacatccgc ctcttcgacc tttttgcccg
     2161 catacatcac gatgacccat tgggaggtct cagctacgac cgcgaggtca tgcgtgatca
     2221 ggatgatggc agtcccgaat tcttgctgca gggcgagcat gagatcgagg atctgcgctt
     2281 ggatggtgac atcgagcgcg gtcgtgggct cgtcggcgat gaggaccttg ggattgcatg
     2341 cgagcgccat ggcgatcatc gcgcgctggc gcatgccgcc cgacagctga tgcgggtatt
     2401 cgttggctcg ctgcgccggt tcgggaatgc ggacacggcg cagcatctcc acggcctttt
     2461 cccaggccgc agctttcgtc aggtcttgat gaaggcgtgc cgtctcgctg atctgcgcgc
     2521 cgatcgtcag caccgggttg agagacgtca tcggctcctg gaagatcatc gagatatcgt
     2581 tgccgcgcac tgcgcgcatt tgcgcttcgt cgagctcgag cagatcccgg ccatcgagct
     2641 cgatcgagcc gccgacgatc cgcccgggcg gatcgggaat gagccgcatc acggagagcg
     2701 ccgtcatgct cttgccgcag ccggattcgc cgacgatcgc cagcgtctcg cgcgggcgca
     2761 acgtgaacga gaggtcgtcg accgctttga tgatcccgag ccgggtaaag aagtatgtcc
     2821 gcagtccggc gacgtcgagg atgggccgtt ccggccgtcc ctcgggacgc gccccccgct
     2881 cggctaccgc tttgttgacg cccgaccgat ccatgtcagc tgcgcagcct cggatcgaga
     2941 atgtcgcgca gcgcgtcgcc gagaaggttg gtgccgaata ccgcgaggct gatggcgata
     3001 ccgggaaaga cgacgagcca cggcgccgtg cgtacatatt cggcggcgga ttgcgacaac
     3061 atgcggcccc aggatggata cggctcgggc accccgagac caaggaacga cagcgcggct
     3121 tcggtgagga tcgtcgaacc gagctgggcg gttgcgagca cgatcaacgg tgcaagggta
     3181 ttgggcagaa tgtgccgcgt tgcgatgcgc agctcgctca tgccgacggc cttcgcagcc
     3241 tcgacgaagg gtagctcacg caacgcaagc gtattggagc ggatgacgcg cgccacatag
     3301 ggaatgagcg ggatcgagat ggcaatgatg gtgttctgca gcgaggggcc gagcgccgcc
     3361 gccatcacga gtgcgagcac caggaggggc agcgcctgca ggatatccat cacgcgttgg
     3421 acgatgaggt cgacccaccc gccgaggtaa ccggacgcga ggccgaagat gatgccgagt
     3481 ccgctcccga gcatcgtcgc gccgatcccg accgcgagcg aaatgcgcgc gccgtagatg
     3541 atccgcgcgt agacgtcgcg cccgaatggg tcggtgccca accagtgatc gctgctcggc
     3601 ggcgcgagcg agagcgctgc attggtcgac aagggatcgt agctggtgag gacgttggca
     3661 aacacggcgg cgaacacgaa cagtcccatg atcaccgcgc cggccgcccc cagcggattg
     3721 cgccaaccaa gataacgcag ccgcgccaat cgtccgtgcg cgccggcgcc ggccttgacg
     3781 agttccgcat cgaagtcgac ggtgctcatc gtgcgctcct ggccgagacc tcagctctcc
     3841 gcgtatttga tgcgggggtc gaggacggca taaagcatgt cgaccgcgaa attgatgagc
     3901 acgacgatcg tggccacgaa catcacgaga ttttgcacga tcgggtagtc gcgctgggtg
     3961 agcgccgtca cgaggtaacg cgcgaccccg ggtacgttga agacggtctc ggtgacgatc
     4021 aagccgccca tgagaaatgc cgcctcgatg ccgatcacgg tgacgaccgg caaaatggcg
     4081 ttccgcaacg cgtgatgata gttgacgctg cgctccgatg ctcccttcgc tcgggcggtg
     4141 cggatataat cttggcgcag cacctccagc atggaggagc gcgtgaggcg cagaatcaag
     4201 gcggagctgc gaaacccgac tgccgcggcc ggaacgctca gcaacaacag ctcatcccac
     4261 cacgtctcgg gcggcgccgt gtagatcgga atcgtgtcga aataggcgac gaacgccatc
     4321 aggatcagca ggccgagcca aaatgacggc atggagaggc cggtcaggct gagcactcgc
     4381 agcaggtagt ccaggccgga attctgatgc actgcgctga tgacgccgag cggaactccg
     4441 atcacgaccg caaagcagag cgcgagtccc gcaagcttgg cggtgaccgg aatgcgcggc
     4501 acaatctcct cgagcgcggg tcgctcggat acgtaggaat agccaaggtc accctgcgcg
     4561 agcccgccga tccaggcgac gtattggacg acgatcggct tatcgaggcc gagctccgcc
     4621 tcgatcttcg ccttttcccg cggattaacg tagccggccg cgtcgtagag gatatcgacg
     4681 atattgccgg gcatcacccg caggagcgcg aagatgatca ccgatatccc gaacagcgtc
     4741 aggagcatca gaaagaggcg ccgcaccagg taagcaccca agggctctat cctttcatcc
     4801 gtgctgcggc cgatcgaatt tgtcgctcgt catggcggtt cgcaaacacg cccggggccg
     4861 aggtctcgtg cggttcccgc ggtctggcgc gtcgggccgc acgtccggtc aagcaagcat
     4921 gctcgcgcgc cgttcgaatc aatgatcgag ccagacgtcc tcgaaacgcc agccgttgta
     4981 cgagctgttg gtcatgatcg tgaccccctt gacccgcggt gaccagcagg tcgcccggcg
     5041 cgaatgcaag atgatcggtc gcgctacgtc ctcctgcaac ttcttgtcga tctcccaaac
     5101 cagctgcttg cgcttttcca gatccgtttc cgccgactgc tggtcgaact gcttctcgag
     5161 ctccttgttg caatagtcgg tgtaattgcg ctccgatccg cagccgtaat tctcgtagaa
     5221 attctggtcc ggatcgtcga ccgagctgcc ggtgaggttg agccccaccg catagtcctt
     5281 gcgcgtgacc ttgctgtgcc agttgctcgt ttccaccact tcgagctcgc cgtcgatata
     5341 gatgtccttg agctgatcga tcaggatgac cgcgggatcg cgatagatgg cgatatttcg
     5401 agtcgagacc tttaccggaa gccgtttgtc gggaccgtaa ccgagcttct ccatcagctt
     5461 gcgcgcctgc gcgcggttcg ccttgatgtc gggcccgtaa cccggaatcg tcttgagaac
     5521 ctcgggtggc atcccccaca cgccccctgg cggtggcagc atggagcccc cgatgtcgtc
     5581 tttgccttcg gagaggatgt cgacaaatgc cttgcggtcg agcgtaagcg ccatggcgcg
     5641 gcgtagatcg gcattgtcga acggaggctt gtcgcggttg acgatgaggt tggtgctgac
     5701 gttgatcgga acaagagaac acagggcttg cggcgcttgc gattttacgt ccttgagaag
     5761 cggaatggtc acctcagttg ggaaggtcat gtcgaacttg ccggcaacga aggcaagaat
     5821 cgcggtcgac cgattgggga tgatggtgaa atcgatgcca tcgagatagg ggcggtcctt
     5881 cttccagtaa tcttcatttc gcgtaatctt gatcgactcg ttctgcttga actccacgaa
     5941 cttgaacgga ccggtgccga tcggcttgac gcgcatgtcg cgcgccgaga cgtgacacgg
     6001 gtagatcggc gaatatccgg aggcaagcag tgcaagcagc gccggctgcg gccgccgcag
     6061 atgaaaggcc acctcatctt cgccgttgac cttgacctcg gcgacgttct tgtaccagtc
     6121 ctggcgtgga ttcttgcgca gcgaatcctt cgacttgccc atcagcaggt cgaaggtgca
     6181 cttgacgtca ttggctgtga acagcttgcc gtcgtgccac ttcacgccct gccgcagctt
     6241 gaacgtcagc tcggtcttgt cctcgctcca cgaccagctg cttgccaggt cgggcacgat
     6301 cgattggaag ctgttctggg cgacgtcctg tttgtacatg acgagattat tgaagatcgg
     6361 catgaacggg atcaccgtcg agtacgtcgc ctcttcgtga atcgacgcgc tcggcggact
     6421 atctcggtga tagatcttca atatgccgcc gtgtttgggc tcgtcggcag cggccgccgt
     6481 gaaggaaact gcggcaatca gcagcgaact cgccgccacc gctcggtgta ccccacgcat
     6541 ggcaaatcct ccctattcgc cccggttgcc gtcgcacagg ttttccggtg cgcgaggccc
     6601 tggacggagt ttcgaatcga cgatcccggc gccccgcgcg gagtgcgggg atcgacggcc
     6661 gaagcgccgc aatcatgggc ccgcccctca tggcccgtgc aacaaacttc tccgcaaatc
     6721 tccccgatgg caaggaattt tgacgcgtct tgtcgacgcg tccatcagtg ttctactgcg
     6781 ctcaaggcgg ccgaaccgct gcggacatcc tcgccgggtg aacaaatggg ccgatccgcg
     6841 acgtccggct ctgtccgttc atgaggtgcg agatcaagcg cgatgtcgcc agaagtaaag
     6901 gtcaagcttg cccggctcat gccatgcggt gtcacttacc atgagatagc acgcgaccgt
     6961 aaaatcgtac gttgagcaaa agagaccgta acccaacgga atttccgtta tcgacaggcc
     7021 cgcaagggaa gcgccggaca gacaatggga tagatcccag agcccatgca tgacatagcc
     7081 aaccgcgata gcaaacctgg aaaatagccc aatcgcagcg caaacaagga acacggcaac
     7141 gccgactgct tccgtttgcc acgcatgcgt tgaggccccg aaataggcag cgaagctcag
     7201 gtagaaggca gcgatcacga caaggaaaga cgcccagaat atctgttccc atcgtccttg
     7261 gaagattcgt cgaaagatga gaatgcttcc aatcgcgctg agcaaaccga taacgacata
     7321 caacagagcc accaccagcc cgccgatgaa ctggctgctc gatccagctc cggccatcag
     7381 attttgtagt agcgctgagt tcattgttgt tgctccgact ctaccgtccc caccccacgg
     7441 ggcactggac tttccatgaa tctcgctcca cgaacctgtg gaggaattca cgcggctgat
     7501 ttcaagccgg ccatgatgct ctgcacttcg ggccaatgtt ccgccccgcg gcgagacatg
     7561 agctcgatat agctcgggtt ttcccacttc ttgatgcccg cttcgaagct tgggcgcgcc
     7621 ttgcagcgcc cgtaccactc ggccactcga ttgcgatcgc tgaccatggc gaaaatgttc
     7681 aggtgctcga gccgagccaa atacggcgta aaggcaacat ccgccaagct gtattcctca
     7741 caggcgagcc agcgatgctc tgcgagcgcc tcctccatct cgtcgaggag ctgtagcatg
     7801 cgctggacag ccgaaacgaa atgttgcgag cgggtgccgt tctcaaccac gtcacgccgg
     7861 cgctggcgtt tgaagatgtt tggaattcgt tcgagcattg cttttccgtg ctcggcgcgc
     7921 tcgagatact gatgacggaa ggcgatcccg aaactgagga tcgccgcgca agcatcatgg
     7981 acatcctcat ccagctgctt gatccatagg cgcatcttcg acctgtggta tggatcgttc
     8041 ggtcgcagcc ccggctgtgg aaacacgtcc tcaagatact cgagaatgac attggactcc
     8101 ggaacaacgt gtccgtcgtg aacgagcgtc ggcaccacgg cgcggcggtt gagcttgagg
     8161 taccacggct gctgatgctc cgccgcccgc agcaccaagt gcttgtcctc ccaggcgagt
     8221 cctttctc
//
LOCUS       DATLJT010001497         1940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2205774,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001497 DATLJT010000000
VERSION     DATLJT010001497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205774"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VM910_34260"
     CDS             complement(<1..218)
                     /locus_tag="VM910_34260"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HVI67608.1"
                     /translation="MVAALVGAAAAVAAVATIPADAQPRRVQVGTLACSISAGVGLVV
                     ASQRNVSCNFQPDGGPPEAYTGTMTRVGV"
     gene            complement(326..1261)
                     /locus_tag="VM910_34265"
     CDS             complement(326..1261)
                     /locus_tag="VM910_34265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67609.1"
                     /translation="MTTGKNSRGMIAVDKIGTRVLFVNPQTYATEVVIDGFPRTVHEL
                     LVVPETGRAYVPIFGDGIHGRNPNPQHHLCVFDLNKRGLVETIDLRPYIAPHTLKLGP
                     DGLIYITCENSAVVAVIDPNSNKVVDAIDSGSTNGHRLIIAPDGQRLYTENEEDGTVS
                     VIDLPERKLLGKIKTPRPLAGIAIAPDGDTVIAVDDAQPTLFLIDTGAGRVREELVLK
                     DVPKPAQIARYAPDNSLLAVTSLNSDTVSLIDPSFREQTAIKVGSQPMDMAFRDGELF
                     VACQGDGSVHIIDIPNKRAKGCFSAGKGCESLGFF"
     gene            1519..>1940
                     /locus_tag="VM910_34270"
     CDS             1519..>1940
                     /locus_tag="VM910_34270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67610.1"
                     /translation="MTAVASRNMKYVSRTDQGGRGDGVQVMVNRGFAYIGHGFSNGIT
                     VIDVRDAKHPKPVNFLPCPPNTRAHHLQTHDDLLLAVNGPSVWTMQVSQEAYFSGTSA
                     AALEGQKFTGGLRIFDISRGDAPREIAFLPLGGLGPHR"
BASE COUNT          358 a          605 c          595 g          382 t
ORIGIN      
        1 acgccgaccc gggtcatggt gccggtatag gcctcgggcg ggccgccatc cggttggaaa
       61 ttgcagctca cattgcgctg cgaggccacg accaggccga cgcccgccga aatcgagcag
      121 gcgagcgtgc cgacctgcac gcgccgcggc tgcgcatcgg ctggaatagt tgcaaccgcc
      181 gctactgctg ccgccgcccc gacgagcgcg gctaccatac ctctacacgc catatctgta
      241 cttgctccct cacaccacga gtcctgccat tattctaggg gagaacgccc ccggcgtctc
      301 gccccgtttt gcgcaaaatg cgttcctaga agaatcccag ggactcgcag cccttgccgg
      361 ctgaaaaaca gcccttggcg cgtttgttcg ggatatcgat gatgtgcacc gagccgtcgc
      421 cctggcaggc gacgaacagt tcgccgtcgc gaaatgccat gtccatgggc tgcgacccga
      481 ccttgatcgc ggtctgctcg cgaaatgaag gatcgatcaa actgaccgtg tcgctgttga
      541 gactggtcac ggcaagaagg ctgttgtccg gcgcgtaacg cgcgatctgc gccggcttcg
      601 gtacatcctt gagcacgagt tcctcgcgca cgcggccggc cccggtatcg atcaaaaaca
      661 aggtcggctg ggcgtcgtcg actgcgatca cggtgtcgcc atccggcgca atcgcgatgc
      721 cggcgagcgg tcttggcgtt ttgattttgc caagcaattt gcgctccggc aggtcgatca
      781 ccgaaaccgt gccgtcttcc tcgttctcgg tatagagacg ctggccgtcc ggcgcgatga
      841 tcagccgatg gccgttagtc gagccggaat cgatggcgtc gacgactttg ttgctgttgg
      901 gatcgatgac cgccacaacc gccgaatttt cgcaagtgat gtagatcaag ccatccggcc
      961 cgagcttaag cgtatgcggc gcgatatagg ggcgcagatc gatggtctcg acaagaccgc
     1021 gcttgtttag atcgaacacg cagagatggt gctgcggatt gggattgcgg ccgtgaatgc
     1081 cgtcgccgaa gatcggcaca taggcgcgac cggtttctgg caccaccagc aactcatgca
     1141 cggtgcgcgg aaagccatcg atcaccactt ccgtcgcata ggtctgcgga ttgacaaaca
     1201 ggactctcgt tccgatcttg tcgaccgcga tcatgccgcg tgagtttttg ccggtggtca
     1261 ttgtttttcc gtgggatggg atcagtagtc tactccgtca tgcccgcgct tgtcgcgggc
     1321 gggtcattcc ggggccgagc caacgggccc gacgcgaagt cgggcggccc gatgacgggc
     1381 tccgcgagga gcccggaatc catagcccct gtatcggaat ccgcaacgga gcgtgtttta
     1441 taacctcgag attcgtgatt atggattccg ggcccgcggg cacagcccgc gtcccggaat
     1501 gacgctcgga ggaatgtgat gaccgccgtt gccagccgga acatgaaata tgtcagccgc
     1561 accgaccaag gcggtcgcgg cgacggcgtt caggtgatgg tcaatcgcgg ctttgcctat
     1621 atcggccacg gtttctccaa tggcatcacc gtgatcgacg tgcgcgacgc caagcatccc
     1681 aaaccggtga atttcctccc ctgcccgccg aatacgcgcg cgcatcacct gcagacccac
     1741 gacgacctgc tgctcgcggt caacggcccc agcgtctgga ccatgcaggt cagtcaggaa
     1801 gcgtattttt ccggcacgtc ggcggccgca ctggaaggac agaagtttac cgggggcttg
     1861 cgcatcttcg atatttcgcg tggggatgcg ccgcgcgaaa tcgcgtttct gccccttggc
     1921 ggcttgggac cgcatcgcat
//
LOCUS       DATLJT010001498         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1748324,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001498 DATLJT010000000
VERSION     DATLJT010001498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1748324"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VM910_34275"
     CDS             complement(<1..442)
                     /locus_tag="VM910_34275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67611.1"
                     /translation="MSRADEQGPLVDLGHERPQLPARARNLSPIVPRASIAGRALVAV
                     VAIMTFLASITTGTVLLVSASAAEWQSDVASEITIQVRPQAGRDVERDTAAVADAIRG
                     QPGIIEVRPFTREESAKLLEPWLGSGLSLDELPVPRVIVARVAPG"
     gene            complement(435..1094)
                     /gene="ftsE"
                     /locus_tag="VM910_34280"
     CDS             complement(435..1094)
                     /gene="ftsE"
                     /locus_tag="VM910_34280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HVI67612.1"
                     /translation="MVRFENVGLRYGLGPEILRDLNFLIPAHSFQFLTGPSGAGKTSL
                     LKLLFLSLRPTRGLVNLFGQDVSLLRKDDIANLRKRIGIVLQDFRLLDHMTTYENVAL
                     PFRVIGRDESSYRKEVIDLLRWVGLGERMDALPPILSGGEKQRAAIARAVISRPQLLL
                     ADEPTGNVDPTLGRRLLRLFIELNKSGTAVVIATHDITLMDQYDARRLVLHQGRLHIY
                     E"
     gene            1345..2199
                     /locus_tag="VM910_34285"
     CDS             1345..2199
                     /locus_tag="VM910_34285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HVI67613.1"
                     /translation="MHIVCPHCTTSYAINSATLGTTGRTVRCSRCKEVWLARPEDAIE
                     PVALQPAMAEASQSDEGAALAAEGTIALREEATDHTPVVESPSIAADWQADASTDPDW
                     PSKARSVAGDDNTGRRARRGFLGKLMARPAGRHTKSSRSLPTVCAAMGALAVALVVWR
                     ADVVRLLPQTASFYKTVGLDVNLRGLSFRDVKVTTETVEGKQVLVIEGAIVGETHKAV
                     ELPRLRFSVRDAQGTEIYAWNTVLDQTVLKPGDHASFKSRLASPPAEGRSIDIRFFNR
                     RDVAGGSV"
     gene            2252..2626
                     /locus_tag="VM910_34290"
     CDS             2252..2626
                     /locus_tag="VM910_34290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI67614.1"
                     /translation="MARVLIADDEDSMRKLVARAITLDGHEAIAAQDGAEALEILVRE
                     EGGFDLLLTDIQMPVMDGIALALSAARDFPQMTILLMTGFAEQRERASNLDAIVHDVI
                     TKPFSVADIRTAVADALAARRP"
     gene            complement(2697..>2995)
                     /locus_tag="VM910_34295"
     CDS             complement(2697..>2995)
                     /locus_tag="VM910_34295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HVI67615.1"
                     /translation="EALRKGAQSMAEAMQQGDGEQGDGPGERAGRQQAGPNSTDPLGR
                     PLRSHEYDELSKMIPGEIDVQRVRRILEELRRRLADPSRPQIELDYIERLLKDY"
BASE COUNT          504 a         1001 c          984 g          506 t
ORIGIN      
        1 caccgggcgc aacccgggcc acgatcacgc gcggcaccgg aagctcgtcg agggacagcc
       61 cgctgccgag ccagggttcc agcaacttgg cggactcctc cctggtgaac ggcctgacct
      121 cgatgatgcc gggctggccg cggatcgcgt ccgcaaccgc cgcagtgtcg cgctcgacgt
      181 cgcggccggc ttgcggccgc acctggatcg tgatctcgct tgcgacatcc gactgccatt
      241 ccgccgccga cgcgctcacc aggagcacgg tgccggtcgt gatcgaggcc aggaaggtca
      301 tgatggcgac gacggcaacc agcgcgcggc ccgcgatcga ggcgcgcggc acgatcggcg
      361 acaggttgcg ggcgcgcgcc ggcagttgcg ggcgctcgtg accgagatcc accagcggcc
      421 cttgctcgtc ggctctactc atagatgtgc aagcgtccct gatgcaggac caggcgccgc
      481 gcgtcgtact ggtccatcaa ggtgatgtcg tgggtcgcga tgacgaccgc ggtccccgac
      541 ttgttgagct cgatgaacag ccgcagcaag cggcgtccaa gcgtcggatc gacattgccg
      601 gtcggctcgt cggccagcaa cagttgaggc cgcgagatca cggcacgcgc gatcgcggcg
      661 cgctgtttct cgccgcccga caggatcggc ggcagcgcgt ccatccgttc tcccaggccc
      721 acccacctga gcaggtcgat cacctccttg cgatagctcg attcatccct gccgatgaca
      781 cggaacggca gcgcgacatt ctcgtaagtg gtcatgtggt cgagcaggcg aaaatcctgc
      841 agcacgattc cgatccgttt gcgcaggttg gcaatgtcgt ccttgcgcag cagcgagacg
      901 tcctggccga acaggttgac cagcccgcgc gtcggccgca gcgacagaaa cagcagtttg
      961 aggagcgacg tcttgcccgc accggacggt ccggtgagaa actggaagga atgggccggg
     1021 atcaggaaat tgaggtcgcg caaaatctcc ggcccgaggc cgtaccgcag tccaacgttt
     1081 tcgaaccgaa ccaagctcta gctccgttcg acatgggccc gccccggaaa aggggaaccg
     1141 ggggcgcgcg agggctcgac gccggtaccc gctctccgga gctcgcgctc catttccgca
     1201 ccgaacttcc acgaacttcg gaaagtcatg ggcgccggca aattagccgg ttttgcgtcc
     1261 gttatggttt ccgattcgtt aacggtcgcc ttgtagcatt tggcagttcc gaatccgaat
     1321 agcgtcatgg cagagagcgg ctccatgcac attgtttgtc cccattgtac aacctcttac
     1381 gccatcaatt cggccacgct cggtacgacc ggccgcaccg tgcgctgctc ccgctgcaag
     1441 gaggtctggc tggcgcggcc ggaggatgcg atcgagccgg tcgcgctgca gcccgcgatg
     1501 gcggaagcga gccaatcgga cgaaggggcc gccctcgctg ccgaagggac cattgccctc
     1561 cgcgaggagg ccaccgatca cacccccgtg gtcgagagcc cgtcgattgc cgcggattgg
     1621 caggccgatg cctcgaccga tcccgactgg ccgtcgaagg cccgttccgt cgcgggcgac
     1681 gataacaccg gacgccgcgc gcgccggggc tttctcggca agctcatggc gcggcccgcg
     1741 ggccggcaca cgaaatcctc ccgcagcctg cccaccgtct gcgccgccat gggcgcgttg
     1801 gcggtggcgc tggtggtctg gcgcgccgac gtggtgcggc tgctaccgca gaccgcttcg
     1861 ttctacaaga cggtcggtct cgacgtgaac ctgcgcggcc tttcgttcag ggacgtcaag
     1921 gtgacgacgg aaaccgtgga gggcaagcag gtcctggtga tcgaaggtgc catcgtcggc
     1981 gaaacgcata aagcggtcga gttgccgcgg ctgcgcttct cggtgcgcga cgcccagggc
     2041 accgaaatct atgcctggaa taccgtgctg gaccagaccg tgctgaagcc cggtgatcac
     2101 gcatcgttca agtcgcgcct cgcctcgccg cctgccgaag ggcgcagcat cgacatccgc
     2161 ttcttcaaca ggcgagatgt ggccggcggc agtgtatgat gcggggccgc gttccctgct
     2221 ttccgcgatt cgcgaacccg agaccgagcc catggcgcgc gttctgatcg ccgacgacga
     2281 ggattccatg cgcaagctgg tcgcgcgcgc gatcaccttg gacggtcatg aggcgatcgc
     2341 cgcgcaggac ggcgccgagg cattggagat cctggttcgc gaggaaggcg gcttcgatct
     2401 cctgctcacc gacatccaga tgccggtcat ggacggaatt gcgctggcgc tgtccgccgc
     2461 gcgcgacttt ccgcagatga cgatcctgtt gatgaccggc tttgccgagc agcgagaacg
     2521 cgcgtccaat ctggacgcca tcgtccatga cgtgatcacg aagccgttct cggtggctga
     2581 catccgcacc gcggttgcgg acgcgctggc ggcgcggcgg ccgtagcgga gtttcgttat
     2641 tgcgaggggc gcaaacggcg ccaacgcggg actgcttcgc tcgcaatgac tgcgagctaa
     2701 taatccttca gcaaccgttc gatatagtcg agctcgatct ggggccgcga cggatcggca
     2761 agacggcgcc gcagctcttc gaggatgcga cggacccgct gcacatcgat ttcgcccggg
     2821 atcatcttcg acagctcgtc atattcgtgc gaccgcagcg gacgcccgag cgggtcggtt
     2881 gagttgggac ccgcctgctg ccggccggcg cgctcgcccg gaccgtcgcc ctgctcaccg
     2941 tcgccctgct gcatcgcctc cgccatgctc tgggcgccct tgcgcagggc ctcca
//
LOCUS       DATLJT010001499         2345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3354837,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001499 DATLJT010000000
VERSION     DATLJT010001499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354837"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VM910_34300"
     CDS             complement(<1..696)
                     /locus_tag="VM910_34300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI67616.1"
                     /translation="MSRVGWIRIAVVAIAIGLLEFCCRLGLIDHRVVIPPSEMADALV
                     RLLILGQANDQIVRTFGIVVVAALISVAAGFVLGVVIHALPRVRQALDPFFATYYAVP
                     IFIFYPVMIVIFGLSVMPIVLMGVATAIVAMIIATLNGLDRIPQVLSKVGRVHRMGTV
                     ATALLLKLPAAAPHLFTGVKLAVSYAFIGVIASEFILAPAGLGRVISDAYTDFNNRQM
                     YALMLLLIIVATVV"
     gene            complement(693..1520)
                     /locus_tag="VM910_34305"
     CDS             complement(693..1520)
                     /locus_tag="VM910_34305"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67617.1"
                     /translation="MTAAISDQDIHASLSGVTRIYPAAAGTQAVHALGQVDLDLKRGE
                     FFAVVGPSGCGKSTLLEVLAGLQRPTAGTVTFEGRPVTGKVPDGIGVVFQEDASFPWL
                     TVWDNAAFGLRRNGVADAEIRRRVDDALAFMGLTDFARAYPAQLSGGMRQRVCISRTL
                     VLQPRLILLDEPFGALDAQTRLLMGDEVLRLWRRTGATILLITHALDEAAMLSDRIGA
                     MSARPGRFLDIVVTGWPRERDSKIVSEAAFGEVTGRLWENLRSESMKSLGYSGVSAE"
     gene            complement(1517..>2345)
                     /locus_tag="VM910_34310"
     CDS             complement(1517..>2345)
                     /locus_tag="VM910_34310"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67618.1"
                     /translation="FFQKHGVDITGIISGAGGGSSVRNAMASDLGYGDVTAAPVIAAA
                     EQGQDVKIVSFTSRSLADLVIIVMPDSPLKTAADLKGKKFGISNPKSLGEMMGVLVME
                     KAGLKQGDVQMTALGSLSGALTALENGVVDVTSIPIILFRTRGGESKYRVLVSPKDLP
                     LIPSQLGMATGQAIKERPGELRAIQAARREGTKFIYEHTDEAIQILSKLYAPLPPKEV
                     GIMVKELVEAKFWTEGPIEMPLLEQTVHAMKGVGMLQNDVDLKKIVDGSFLPPDLQK"
BASE COUNT          462 a          761 c          728 g          394 t
ORIGIN      
        1 gacgaccgtg gctacgatga tcaacagcag catcagcgcg tacatctgcc gattgttgaa
       61 gtcggtatag gcgtcggaaa tgacgcgccc cagtcccgcg ggtgcaagga tgaactcgct
      121 ggcgatgacg ccgatgaaag catagctgac ggcgagcttg acgccggtaa agagatgcgg
      181 cgccgccgcc ggtaatttca ggagcagcgc cgtggcgacg gtccccatgc gatggacccg
      241 ccccaccttg gagagcacct gcggtatgcg gtcgagcccg ttcaatgttg cgatgatcat
      301 cgccacgatc gcagtcgcca ctcccatcag cacaatcggc atgaccgaga gcccgaagat
      361 cacgatcatc accgggtaga agatgaagat cggaaccgca taataggtgg cgaagaacgg
      421 atcgagcgcc tgccgcacgc gcggcagcgc gtgaatgacg acaccgagca caaagcccgc
      481 cgccacggaa atcaaggccg ctacgacgac gatgccgaat gtgcggacga tctggtcatt
      541 ggcctgcccc aagatcagaa gacgaaccag cgcgtcggcc atctccgagg gcggaatcac
      601 gacgcgatga tcgatcaggc caaggcggca gcagaattcg agcagcccga tcgcgatcgc
      661 aacgaccgca atcctgatcc agccgacccg actcattcgg ccgacacccc ggaatagccg
      721 agcgatttca tcgattcgct gcggaggttc tcccacagcc ggccggtgac ctcgccgaac
      781 gctgcttcgc tgacgatctt gctgtcgcgc tcgcgcggcc agcccgttac gacaatgtca
      841 aggaatcgcc ccggccgggc cgacatcgca ccgatgcggt ccgacagcat cgcggcctcg
      901 tcgagcgcgt gcgtgatcag gagaatggtc gcgccggtgc gccgccacag gcgcagcacc
      961 tcgtcgccca tgagcagcct tgtctgtgca tcgagggcgc cgaacggctc gtcgagcaga
     1021 atcaggcgtg gctgaagcac gagcgtgcgg gagatgcaga cgcgctgccg cataccgccc
     1081 gaaagctgcg cgggataggc gcgggcgaag tccgtcaatc ccatgaaagc cagcgcatca
     1141 tcgacgcgac gacgaatctc cgcgtcggct acgccgttac gacgcaagcc gaacgcggcg
     1201 ttgtcccaca cggtcagcca cggaaagctg gcgtcttcct gaaagacgac gccgatgccg
     1261 tccggcacct tgccggtgac cggacggccc tcgaacgtca ccgtgccggc ggtgggccgc
     1321 tggaggccgg ccagcacctc caggagcgtg gacttgccgc agccggacgg tccgacgacg
     1381 gcgaagaatt cgccgcgctt gaggtcaaga tcgacctgtc cgagcgcatg aaccgcttgc
     1441 gtcccggcgg ccgccggata gatccgcgtt acacccgaga gggaggcgtg gatgtcctga
     1501 tccgaaatcg cggccgtcat ttttgcagat cgggcggcag gaatgagccg tcgacgatct
     1561 tcttgaggtc gacgtcgttc tgcagcattc cgacgccttt catcgcatgc acggtctgct
     1621 cgagcagcgg catctcgatc gggccctcgg tccagaactt cgcctccacg agctccttga
     1681 ccatgatgcc gacctccttc ggtggcagcg gcgcatagag cttgctgagt atctggatag
     1741 cctcgtcggt atgctcgtag atgaacttgg tcccttcgcg acgcgccgcc tggatggcgc
     1801 gcagctctcc cggcctctcc tttattgcct ggccggtggc catgccaagc tgcgacggaa
     1861 tgagcggcaa gtctttcgga ctgacgagca cgcgatactt gctctcgccg ccgcgggtcc
     1921 gaaacagaat gatggggatg gacgtgacgt ctaccacgcc attctccaac gcagtgagcg
     1981 cgccgctgag actgccaagc gccgtcatct gcacgtcgcc ctgcttgagt cccgccttct
     2041 ccatcaccag cactcccatc atctcaccaa gcgatttcgg gttgctgatg ccgaacttct
     2101 tgcctttgag atcggccgca gtcttgagcg gtgaatcggg catgacgatt atcaccaggt
     2161 cggccagcga gcgcgaggtg aaactgacaa tcttgacgtc ctgcccctgc tcggccgcgg
     2221 cgatgaccgg ggcagccgtg acgtcgccgt agccgagatc gcttgccatg gcattgcgca
     2281 ccgaactgcc gccgcctgcg ccggagataa tgccggtgat gtcgacaccg tgcttctgga
     2341 agaag
//
LOCUS       DATLJT010001500         5743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_137756,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001500 DATLJT010000000
VERSION     DATLJT010001500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137756"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VM910_34315"
     CDS             complement(<1..831)
                     /locus_tag="VM910_34315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVI67619.1"
                     /translation="MLEKFSRRHFVQLAGLSGMAIAAPRALAQERQTAPALAPDAAGA
                     FPNGFIWGTATSAYQIEGAVNEDGRGPSIWDIFSHTPGKIGDQTNADRANDHYHLYKD
                     DVRLIKELGVKAYRFSIAWPRVFPQGDGPSNPKGLDFYNRLIDELLANGIEPYATLYH
                     WDLPQSLQERFGGWQSSTTSKAFADYAGYVTERLSDRVKNFFTLNEAGRFLNFGYGWG
                     IDAPGLKLPTGELNQARHHVVLGHGLAVQAIRARARAGTKLGLAENIAACVPAIDTPD
                     N"
     gene            complement(878..1852)
                     /locus_tag="VM910_34320"
     CDS             complement(878..1852)
                     /locus_tag="VM910_34320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HVI67620.1"
                     /translation="MLKHLTIAFLLATLAPAFAQDDLGAAPSALSLQSTRDELGGGCR
                     RVDRLDSAQFAPVALHRTFHDDFDEHPLKSGRWVPHYSGGAAWPEARYWGGDGSELKR
                     QTKSNGEQQIYVDPRYTGRASTPLGLDPFRIRDGVLSIVASRTPAELKSVLFDNPYVS
                     GILTSQASFAQKYGYFEIRAKIPHGTGVWPAFWLLANDGGWPPEIDVMEGRGQRPGDL
                     VMTTHWRIPTTQKIESCGFDFLLPDASMDFHDYGILWVPDRLIYFIDRKPVSDIKVPV
                     GYDDPMYMIVNLAMGSKWFGGVGVVDGMSPEMVEFEIDRISVYQIDPN"
     gene            complement(1903..2682)
                     /gene="cobS"
                     /locus_tag="VM910_34325"
     CDS             complement(1903..2682)
                     /gene="cobS"
                     /locus_tag="VM910_34325"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HVI67621.1"
                     /translation="MADTIHTMRRLLADLRIGISLCTRVPLAPAAPVDDGEVARASWT
                     FPIAGFVVGLVGAFVYWLAVRLHVPPEPAAVLALTATVLVTGAMHEDGLADTADALGG
                     STRERKLQIMRDSRIGTFGTAALALSFLLRWTALAEIAEMADARAIAIALIVAHTASR
                     AVLPAFMYLVPPARADGLSTSAGRPPQQSVVVSLGLGILCLLFAFGPKLATIALLMLA
                     LAALVLARLAVRQIGGQTGDVLGAQEQIGEAILLLIASTMI"
     gene            complement(2685..3188)
                     /gene="cobU"
                     /locus_tag="VM910_34330"
     CDS             complement(2685..3188)
                     /gene="cobU"
                     /locus_tag="VM910_34330"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HVI67622.1"
                     /translation="MAIILITGGARSGKSVRAEARARAFAGKPVYIATAEALDAEMAD
                     RIARHRARRGNEWLERETPLELVAALDETDGGGARLVDCLTLWLSNLLHAGRDWTSAT
                     AALADALARQRSPVVLVTNEVGLGIVPDNALARRFRDAAGIMNQTIARAADEVEFVVA
                     GLPVRIK"
     gene            3321..4259
                     /gene="cbiB"
                     /locus_tag="VM910_34335"
     CDS             3321..4259
                     /gene="cbiB"
                     /locus_tag="VM910_34335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HVI67623.1"
                     /translation="MGFAGAMAVAMVLEGLLGWPNSLVARIGHPVTWLGRLIDALDAR
                     WNREKAAPSIRRAAGVAAALVVLGLAAGLGWLLQSLISPWDRVVLPGILAMPFIASRS
                     LYEHVVAVARPLEAGDIASARSAVAMIVGRDPARLDETGIARTALESLAENASDGIVA
                     PVFWGALFGLPGIIGYKAVNTLDSMIGHRTARYEAFGWASARIDDVANAVPARLTGLL
                     FALISARPGNAFACMFRDARKHRSINAGWPEAAMAGGLAVRLCGPRFYDGELADEPWL
                     NEGARDPNAADITEGLRLYIRAMASITALLVLLAFV"
     gene            4270..5235
                     /gene="cobD"
                     /locus_tag="VM910_34340"
     CDS             4270..5235
                     /gene="cobD"
                     /locus_tag="VM910_34340"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HVI67624.1"
                     /translation="MQDHGGNLDVALARFGGRFEDWIDLSTGVNRRPYPVGELPPHRL
                     SALPSRSDLTSLHDAARQAYGTTAPALAVAGAQAAIQLLPRLADPARARVLAPTYSEY
                     LPTLKAAGWQADEVSDLDALAGSDLAVVVNPNNPDGRRHEPARLLALLPRVGRLVVDE
                     SFVDAVPGRSLAAAAGRPGLLILRSFGKFYGLAGLRLGFVLGCERDVAAFAALAGPWP
                     VSGVAIEIGRRALCDCDWAAETTARLAYDSLRLDDLACDAGWTLVGGTPLFRLYETGD
                     AHSAQERLARAHIWSRIFRDAPGWLRLGLPGDETEWARLAAALAS"
     gene            complement(5279..>5743)
                     /locus_tag="VM910_34345"
     CDS             complement(5279..>5743)
                     /locus_tag="VM910_34345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVI67625.1"
                     /translation="NLSFGYVAYDAGVGYTYFDPKTGHEFSVVGGLTYNLINPDLQYQ
                     NGIDFHLDWAASQFVSKNVQIGLAGYFFQQLTGDSGPGATLGPFKGRTVGIGPQIGFI
                     FPVSEGYQGYLNLRGYKDLAVENRPEGWTAWLTFAVSPAPPEAPSKPLVRKY"
BASE COUNT          972 a         1824 c         1917 g         1030 t
ORIGIN      
        1 attgtcgggc gtatcgatcg cgggcacgca ggccgcgatg ttttcggcaa gccccagttt
       61 cgtgccggcg cgcgcgcgag cgcggatcgc ctgcaccgca agcccatggc ccagcacgac
      121 gtgatgacgc gcctgattca attcgcccgt cggcagcttg agaccgggtg catcgatccc
      181 ccagccatag ccgaagttga gaaaacgtcc ggcttcgttg agcgtgaaga aatttttgac
      241 gcgatcgctc aaccgctccg tcacgtagcc ggcatagtcg gcgaacgcct tcgacgtcgt
      301 gctcgactgc caaccgccaa agcgctcctg cagtgattgc ggcagatccc aatgataaag
      361 cgtggcgtag ggctcgatgc cgttggcgag aagctcgtcg atcagacggt tgtagaaatc
      421 gaggcctttc ggattggacg gcccgtcgcc ttgcggaaat acacgcggcc aggcgatcga
      481 gaagcgataa gccttcacgc ccaattcctt gatcaggcgg acgtcgtcct tgtagagatg
      541 atagtgatcg ttggcacgat cggcgttggt ttggtcgccg atcttgcccg gcgtgtggct
      601 gaagatgtcc cagatcgagg gaccgcggcc gtcctcgttg acggcccctt cgatctgata
      661 ggccgaggtc gcggtgcccc agatgaaccc gttcggaaac gcccctgctg cgtccggcgc
      721 gagcgccgga gccgtctggc gttcctgcgc gagcgcgcgc ggggccgcaa ttgccatgcc
      781 ggacagccct gcaagctgga cgaagtgacg acgcgagaat ttttcgagca tcgactggtc
      841 tcgacaggtt cgctgtgacc ggcactcgat aatagagcta gttcggatcg atttggtaga
      901 cggaaatccg gtcgatctcg aattccacca tctcgggcga catgccgtcg accacgccga
      961 cgccgccgaa ccatttcgat cccatcgcca gattgacgat catgtacatg ggatcgtcgt
     1021 agccgaccgg caccttgata tcggacaccg gcttgcggtc gatgaaatag atcaggcggt
     1081 ccggcaccca gagaatgccg taatcgtgga agtccatcga ggcgtccggc aacagaaagt
     1141 caaaaccgca ggattcgatt ttctgggtcg tggggattcg ccaatgcgtc gtcatcacga
     1201 ggtcgccggg ccgttgccca cgcccctcca tcacgtcgat ctccggcggc cagccgccgt
     1261 cattggcaag cagccagaag gcgggccata cgccggtccc gtgcggaatt tttgcgcgga
     1321 tctcgaaata tccgtatttc tgcgcgaacg aggcctggct ggtcaggata cccgacacat
     1381 agggattgtc gaacaacacg gatttcagtt cggcgggcgt gcggcttgcg acgatcgaca
     1441 gcacgccgtc gcgaatccgg aacggatcga gtccgagcgg cgtcgaggcg cggcccgtgt
     1501 agcgcggatc gacgtagatc tgctgctcgc cattggattt ggtctgtcgc ttcagttcgg
     1561 agccgtcgcc gccccaataa cgcgcctccg gccacgccgc gccgccggaa taatgcggca
     1621 cccatctgcc gctcttcagc ggatgctcgt cgaaatcgtc gtgaaaggtg cgatgcaggg
     1681 cgacgggcgc aaactgcgcg gaatccagcc ggtcgacgcg gcggcagccg ccgccgagct
     1741 cgtcgcgggt cgattgcagc gagagcgctg aaggcgctgc tccgagatca tcctgcgcga
     1801 aagcgggtgc aagcgtcgca agcaaaaagg cgatggtcag atgtttcagc atgcatcatt
     1861 tccgatgccc ccggccgttt ttccgatgcg ccccctgtca gtttagatca tcgtcgatgc
     1921 gatcagaagc aggatggctt cgccgatctg ctcctgcgcg cccagcacgt cgccggtttg
     1981 gccgccgatt tgccggaccg caaggcgcgc cagcacgagc gcggcgagcg cgagcatgag
     2041 cagcgcgatg gtcgcaagct tcggtccgaa cgcaaacagc aggcagagga tgccgagccc
     2101 aagcgatacg acaacgcttt gctggggcgg ccgccccgcg ctggtcgaca atccgtcggc
     2161 gcgggcagga ggaaccaggt acatgaaggc cggcaacacg gcgcgcgagg ccgtgtgcgc
     2221 cacgatcagc gcaatcgcga ttgcgcgggc atcggccatc tcggcgattt cggcgagcgc
     2281 ggtccagcgc agcaggaacg acagcgcaag tgcggccgtg ccgaaggtcc cgatccggct
     2341 gtcgcgcatg atctgaagct ttcgctcgcg cgtgctgccg ccgagcgcgt cggcagtgtc
     2401 ggcgagcccg tcctcatgca tcgcgccggt gaccagcacg gtcgccgtca gcgccagcac
     2461 ggcggcaggc tccggcggaa cgtgcaagcg gaccgcaagc caatagacga aggcgccgac
     2521 gaggccgacc acgaaccccg caatcgggaa cgtccagctc gcgcgcgcaa cctcgccgtc
     2581 gtcgaccggc gctgcgggcg caagcggcac gcgggtgcaa agcgaaattc cgatacggag
     2641 gtcggcgagc aggcgccgca ttgtatggat ggtgtcggcc atcgctactt tatccgcacc
     2701 ggcaggcccg cgaccacgaa ttccacttcg tccgcggcgc gggcgatcgt ctggttcatg
     2761 atgccggcgg cgtcacgaaa gcggcgcgcc agcgcattgt cgggcacgat gccgagtccg
     2821 acttcgttgg tgacgagcac gaccgggctc cgttgccggg caagcgcgtc ggcgagtgcg
     2881 gcggtcgcgc tcgtccagtc gcgcccggca tgcaagaggt tggagagcca aagggtcagg
     2941 cagtcgacga gccgcgcgcc gccgccgtcg gtctcatcga gcgctgcgac aagctccagc
     3001 ggcgtttccc gctcgagcca ctcgttgccg cgccgcgcgc ggtgacgcgc gatccggtcg
     3061 gccatctcgg catcgagcgc ttcggcggtt gcgatataga ccggcttgcc ggcaaaggca
     3121 cgcgcgcggg cttctgctcg cacgctcttt cccgatcgcg ccccgcccgt gatgaggatg
     3181 atggccatgg ccgctccgat tcgccatgcg cactaatcat caattcgcgg caatgacaaa
     3241 cggcgaaatc gtctcctcaa agggcttgcg cccgccgttc attttcgcgc atcaaaatgg
     3301 gacaaccacg gaggttttca ttgggctttg caggggcgat ggccgttgcg atggttttgg
     3361 aggggctgct cggctggccg aacagcctcg tcgcgcgcat cggtcatccc gtgacctggc
     3421 tcggccggct gatcgacgcg ctcgacgcac gctggaaccg ggaaaaggca gcgccatcca
     3481 tccgccgcgc cgccggggtc gccgccgcgc ttgtcgtgct cggactcgcg gcaggtctcg
     3541 gatggctcct gcagagcctg atctcgccct gggatcgcgt ggtcctgccc gggatcctcg
     3601 cgatgccttt catcgcctcg cgctcgctct acgagcatgt cgtcgcagta gcacgcccgc
     3661 tcgaggcggg cgatatcgca tcggcgcgat cggcggttgc catgatcgtc gggcgcgatc
     3721 cagcaaggct cgacgagaca gggatcgcgc gcaccgcgct ggagagtctg gcggaaaatg
     3781 cgtccgatgg catcgttgcg ccggtgttct ggggcgcatt attcgggctt cccggcatca
     3841 tcggctacaa ggccgtcaac acgctggatt ccatgatcgg ccatcgcacc gcacgctacg
     3901 aggcgttcgg ctgggcctcc gcgcgcatcg acgatgtcgc gaatgcggtg ccggcgcggc
     3961 tgaccggcct gctgttcgca ctgatcagcg cccgcccggg gaatgcgttc gcctgcatgt
     4021 ttcgcgatgc gcgaaaacac cgttcgatca atgccggctg gccggaagcc gcgatggcag
     4081 gcgggcttgc tgtgcggctg tgcgggccgc gcttctatga cggcgaactt gccgatgagc
     4141 cctggctgaa cgaaggcgcg cgtgatccga acgctgctga tatcacggag ggcttgcggc
     4201 tctatatccg cgcgatggcg agcatcacgg cgcttcttgt gctgctggcg ttcgtctaaa
     4261 gagataacga tgcaggacca tggcggaaat ctcgatgtcg cgctcgcccg ctttggcggg
     4321 cggttcgagg actggatcga tttgtcgacc ggcgtcaacc ggcggcccta tccggtcggc
     4381 gagttgccgc cgcatcgcct gagcgcgctg ccctcgcgct ccgatctgac gtccctgcac
     4441 gacgcggcgc ggcaggccta tggaacgacg gcgccggcat tggccgtcgc cggggcgcag
     4501 gctgcgatcc agttgctgcc gcgcctggcc gatccggccc gcgcacgcgt tcttgcgccg
     4561 acctatagcg agtacctgcc gacgttgaag gccgccggct ggcaggccga cgaggtttcc
     4621 gatctcgacg cgctggctgg aagcgatctt gcggtcgtcg tcaatcccaa caatcccgac
     4681 gggcgtcgcc acgagccggc gcgcttgctg gcactgttgc cgcgcgtcgg ccggctcgtc
     4741 gttgacgaga gttttgtcga cgcggtgccc ggaaggtcgc tggcggccgc tgcgggacgg
     4801 cccggccttc tgatcctgcg ctccttcggc aaattttacg ggcttgccgg gctgcggctt
     4861 ggcttcgtgc tcggttgcga gagggatgtg gcggcgttcg ccgcgctcgc cggtccctgg
     4921 ccggtctcgg gcgttgcgat cgagatcggc cggcgcgctt tgtgcgattg cgactgggca
     4981 gcggagacga cggcgcggct tgcgtacgat tcgctgcggc tcgacgatct cgcctgcgat
     5041 gcgggctgga cgctggtggg cggcacgccg ctgtttcggc tttacgaaac gggcgatgcg
     5101 cacagcgcgc aggagcggct cgcccgtgca cacatctggt cgcgcatctt cagggacgcg
     5161 ccgggatggt tgcgcctggg tttgcccggg gatgaaacgg agtgggcacg gctcgccgcg
     5221 gcgcttgcga gctagattag ccagggcgtg tacgcaaatc cgtatcggct tgcgactttc
     5281 aatatttgcg aacgagcggc tttgacgggg cctccggcgg cgccggcgaa accgcgaacg
     5341 tcaaccatgc ggtccatcct tcggggcggt tctccacggc aagatcctta taacccctca
     5401 gattgagata gccctgatag ccctcgctga cgggaaagat gaaaccgatt tgcgggccga
     5461 tgccgacagt ccggcccttg aacgggccca gcgtggcgcc cggcccactg tcgcccgtaa
     5521 gctgctggaa gaagtagccg gccaatccaa tctggacgtt cttgctcacg aactgcgaag
     5581 ctgcccagtc gaggtgaaaa tcgatgccat tctggtattg cagatcggga ttgatgagat
     5641 tgtaggtgag gcctccgaca accgagaact cgtgtccggt ctttggatcg aaataggtgt
     5701 agccgacgcc ggcatcgtac gccacgtagc cgaagctcag att
//
LOCUS       DATLJT010001501         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4274634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001501 DATLJT010000000
VERSION     DATLJT010001501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4274634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VM910_34350"
     CDS             complement(<1..300)
                     /locus_tag="VM910_34350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67626.1"
                     /translation="MARKGANSPTGGRKLRSTGTKARGHVSNGPNPIKLKKQLEARTR
                     ELAEARGQLSEALEQQTATSEVLQVISRSPGELQPVFESMLANATRLCGAKFGTLN"
     gene            complement(410..1411)
                     /locus_tag="VM910_34355"
     CDS             complement(410..1411)
                     /locus_tag="VM910_34355"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67627.1"
                     /translation="MNRRRIISLIGGAAAAWPLAALAQQRATAQAQEPETPVIGFLGA
                     VSPDGFTERVRAFRQGLKEGGFVEGENVGIEYRWAENQLNRLPALAADLVRKRVTVIF
                     ANGGTAPAIAAKAATTTIPIVFAIPEDPVSLGLVASLSRPGANLTGVNFFFGELVPKR
                     LELLRELVPALTQVAVFVNPANPSRAEFLVREAESAGRTMDLQIQVFNTGTVSEINTA
                     FTTLANHRPDALLVTPDPFFIGRRAQIATLAARHGVPTSFPARDYVEAGGLMSYGPNI
                     NDAFHQAGVYTGRVLKGAKVADLPVVQSAKFELVINAQTARMLGLTVPDKLLATLTR"
     gene            complement(1499..2587)
                     /locus_tag="VM910_34360"
     CDS             complement(1499..2587)
                     /locus_tag="VM910_34360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI67628.1"
                     /translation="MKRREFLKIAATGMAASAVAAPAIAQSTPAIRWRLTASWPKSLD
                     TLYGACETFAKYVSEATDSKFQVQVFAAGEIVPALQVLDAVQTGTVEIGHSATYYFIG
                     KDPTWALFCAVPFGLNTRQLNAWFYDGDGMKLMIEFSKKFSVYPLVGGNTGAQMGGWF
                     RKEIKAVEDLKGLKFRIGGWAGKTLAKLGVVPQQIAGGDIYPALEKGTIDAAEWVGPY
                     DDEKLGFYKIAPYYYYPGWWEGSAAAHFLFNEAKWNELPKTYQTTVTAAAGFANLEMT
                     GRYDARNPVALKRLVAAGAQLRPFPQPVMEACLKASNEVNAETSATNADYKKVLDSMQ
                     AFRNDEYLWWQVAEYTYDSFMIRTRTRT"
     gene            complement(2584..>2782)
                     /locus_tag="VM910_34365"
     CDS             complement(2584..>2782)
                     /locus_tag="VM910_34365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67629.1"
                     /translation="THLAREGRMTVTIGRWELLAALGGVAAAWLLAAAGDAGGWVPQR
                     QRARRHATTPAVTPVNEEIDP"
BASE COUNT          492 a          894 c          848 g          548 t
ORIGIN      
        1 gttcagcgtg ccaaacttcg caccgcagag ccgagttgcg tttgccagca tggactcgaa
       61 aacaggctgc agctcgccgg gcgagcggga gatgacttgc agtacctccg aggtcgccgt
      121 ctgctgctcc agcgcctcgg aaagctgccc ccgcgcctcg gcgagctcac gggtgcgcgc
      181 ttcgagttgc tttttcaact tgatcggatt gggcccgttg ctaacgtgcc ctctcgcctt
      241 cgttccggtc gaacgaagct tgcggccgcc cgttgggcta tttgcgcctt tgcgagccaa
      301 ggtcacctct tcggcacagc cactggtccc cctttcagtc cgcccagcca aggatcgagc
      361 ctatcaatcg taacagaacc gcatgatgaa ctcgcgcctc ttcactcgat cacctcgtca
      421 gggtcgcgag cagcttgtcc ggcacagtga ggccgagcat cctggccgtc tgagcgttga
      481 tcaccagctc gaacttggcc gactgtacga ccggcaaatc cgcgaccttc gcgcccttga
      541 ggacgcggcc agtgtagacg ccagcttgat ggaaggcgtc gttgatgttg ggtccgtagc
      601 tcatcagtcc gcccgcctcg acatagtcgc gcgccggaaa cgacgtagga accccatgac
      661 gtgccgccag cgtggcaatt tgtgcgcgtc tgccaatgaa gaagggatcc ggggtgacca
      721 acagcgcgtc gggccggtgg tttgcaagtg ttgtgaacgc cgtgttgatc tcgctgacag
      781 tgcctgtgtt gaagacttgg atttgcagat ccatcgtgcg tcctgcggat tccgcctccc
      841 tcacgaggaa ctccgcacgc gaaggattgg ctggattgac gaagaccgcg acctgggtca
      901 atgcgggaac cagctcgcgc aggagctcca gccgcttcgg caccaactcg ccgaagaaga
      961 agttgacgcc ggtgaggttg gctcccggcc gggaaaggct ggcgacaagc ccaaggctaa
     1021 cggggtcttc cgggatcgcg aaaacgatcg gaatagtcgt tgttgccgcc ttggctgcaa
     1081 tcgccggagc ggtaccgccg ttcgcgaaga tgactgtgac ccgcttgcga accagatcgg
     1141 ccgccagcgc cggtaaccgg ttgagttggt tctcggccca gcggtattca atcccgacgt
     1201 tctcgccctc gacgaagccc ccctccttga ggccctgccg aaatgcgcgc acacgctccg
     1261 taaagccgtc aggcgatact gcgccaagga atccgatcac cggcgtttct ggctcctgcg
     1321 cttgcgccgt ggcccgctgc tgtgcgagcg ccgcgagcgg ccacgcagct gcagcgccgc
     1381 cgatgagcga tatgatcctg cggcggttca ctcgatcacc tcgtccactg tagcgagcat
     1441 aacggagtcg accgtcgggc gcggcctcgt ggtggggctg caaccgatgc gcgcgcggct
     1501 acgtccgcgt ccgcgtgcgg atcatgaagc tatcataggt gtattccgcg acctgccacc
     1561 acagatactc gtcgttgcgg aatgcctgca tcgagtccaa caccttcttg taatcggcgt
     1621 tggtcgccga ggtctcggca ttgacctcgt tcgacgcctt caggcacgct tccatgacgg
     1681 gttggggaaa tggacgcagt tgcgcaccgg ccgcgactag gcgcttcaac gccaccgggt
     1741 tgcgggcgtc gtatctcccc gtcatttcaa gattggcgaa acccgcggcc gcggtgaccg
     1801 tcgtctgata ggtcttcggc aattcgttcc attttgcctc attgaatagg aagtgcgccg
     1861 cggcgctgcc ctcccaccaa ccgggataat agtagtatgg cgcaatcttg tagaacccga
     1921 gcttctcgtc gtcatacggg ccgacccact cggcggcgtc gatggtcccc ttctccagcg
     1981 ccggataaat gtcgccgcca gcgatctgct gcggcacgac cccgagcttc gcgagcgtct
     2041 tgccggccca gccgccgatg cggaatttga gacctttgag atcctcgaca gccttgattt
     2101 ccttgcggaa ccagccaccc atctgcgcgc cggtattgcc tccgaccagc gggtacacgc
     2161 tgaatttctt gctgaactcg atcatcaact tcatgccgtc gccgtcgtaa aaccaggcgt
     2221 tcagctggcg ggtattgagg ccgaacggca cggcgcaaaa cagcgcccag gtcgggtcct
     2281 tgccgatgaa atagtaggtg gcgctgtggc cgatctcgac ggtgccagtt tgaaccgcgt
     2341 cgagcacctg caacgcaggc acgatctcgc ccgcggcgaa gacttgaacc tgaaacttgc
     2401 tgtcggtcgc ctcggaaaca tacttggcga acgtttcgca agcgccgtag agggtgtcga
     2461 gcgattttgg ccagctcgcc gtcaatcgcc atcggattgc cggcgtcgac tgggcaatgg
     2521 cgggcgcagc caccgctgat gcggccattc cggtggcggc aatcttgaga aattcgcggc
     2581 gcttcatggg tctatttcct cattcacagg cgtaactgca ggagtcgtgg cgtgtcgccg
     2641 ggcgcgctgg cgctgaggaa cccagccacc ggcatcgccg gctgctgcga gcagccacgc
     2701 cgccgccacg ccgccgagtg cggccagcaa ttcccatcgt ccgatggtga ctgtcatacg
     2761 cccctcccgt gcgaggtgcg ta
//
LOCUS       DATLJT010001502         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1057324,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001502 DATLJT010000000
VERSION     DATLJT010001502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057324"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VM910_34370"
     CDS             <1..1068
                     /locus_tag="VM910_34370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67630.1"
                     /translation="QTIEDLKFNNYKKEMFLDSDTKISLISSAPSDIEQDWFLTNEQM
                     AQARKQINDEAGSRRVFCHAIFTPGQPGWLDKLDAALALKPESCKGYTIGDNTHKDIS
                     RYPWRLDDEKIAYKGYEKMVNAGIKNVCVHKGLFPPGIEKQFPNLRGFADVADVGQAA
                     KDWPQLNFVIYHSAYRHVGGDPKVALAEFNRTGRIAWTSDLADIPAQYGVSNVYGDVG
                     QLFATTLVAEPNVCAALMGTLIKGLGADHICWGTDALWTGAPQWQIEGLRRLEIPEDM
                     QKKFGYAPLGPADGPVKTAIFGDNNARLYGIQPKRAMLELKGDRFALMKAKYEKAGAE
                     PSNTRYGYVVPNGPIDHRVFA"
     gene            complement(1149..1886)
                     /locus_tag="VM910_34375"
     CDS             complement(1149..1886)
                     /locus_tag="VM910_34375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI67631.1"
                     /translation="MTLVRYESAERVATITMDRREKHNALNGALCEELRQAWLTFRDS
                     DDRVAVLASAEDAYFSVGADVKDFPVNMWHAVPGLGVELDKPVIAATSGWVVGGGFVL
                     VQMADMCIASETTRFTYPEAKIGTTAGGISSVVARMPHKIAMEFLLVGEELSVERAYQ
                     IGFVNRIAPKGQHIVQAQEMAAKIAANAPLVVQSLKKLARDVMPKGPLETVAEVRRLL
                     DAVRESDDFREGVKAFGEKRKPQFKGK"
     gene            complement(2017..>2425)
                     /locus_tag="VM910_34380"
     CDS             complement(2017..>2425)
                     /locus_tag="VM910_34380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67632.1"
                     /translation="VPSTPGMDRKAAINFARVGAQKLWAGTVTIKPDAKTGAHHHGHL
                     ESIIYVVKGKARMRWGEHLQFTAEAGPGDFIYVPPYVPHQEINASRDEVLECVLVRSD
                     GEAIAINLDIEPVEKPETVLWVDPVHRHPDEKK"
BASE COUNT          446 a          785 c          743 g          451 t
ORIGIN      
        1 cagaccatcg aggatttgaa gttcaacaat tacaagaagg agatgttcct cgattccgac
       61 accaagatct cgctgatctc gtccgcgccg tccgacatcg agcaggactg gttcctgacc
      121 aatgagcaga tggcgcaagc gcgcaagcag atcaacgacg aggcgggctc gcgacgggtg
      181 ttctgccacg cgatcttcac gcccggccag ccgggctggc tcgacaagct cgacgccgcg
      241 ctcgcgctga agccggaatc ctgcaagggc tacacgatcg gcgacaacac gcacaaggac
      301 atcagccgct atccctggcg gctcgacgac gagaagatcg cctacaaggg ctacgagaag
      361 atggtgaatg cgggcatcaa gaatgtctgc gtgcacaagg ggctgtttcc gcccggcatc
      421 gaaaagcagt tcccgaattt gcgcggcttt gccgacgttg ccgacgtcgg acaggccgct
      481 aaggactggc cgcagctcaa cttcgtcatc taccattcgg cctaccggca tgtcggcggc
      541 gatccgaagg tggcgcttgc cgaattcaat cgcaccggcc gcattgcctg gaccagcgac
      601 ctcgccgaca ttcccgcgca atatggcgtg agcaacgtct atggcgacgt cggccaattg
      661 ttcgcaacga cgctggtcgc ggagccgaat gtctgcgcgg cgctgatggg cacgctgata
      721 aagggccttg gcgccgatca tatctgctgg ggcaccgacg ccttgtggac cggcgcaccg
      781 caatggcaga tcgagggatt gcggcggctc gaaattccgg aggacatgca gaagaagttc
      841 ggctacgcgc cgctcggccc cgccgacggt ccggtcaaaa ctgcgatctt cggcgacaac
      901 aatgcgcggc tctacggcat ccagcccaag cgcgcgatgc tcgagctgaa gggcgaccgc
      961 ttcgccctga tgaaggcgaa atacgagaag gcaggtgccg aaccgtcgaa cacgcgttac
     1021 ggctatgtcg tgccgaacgg acccatcgac caccgggtgt ttgcgtaaag ccccgcgacg
     1081 tcattggaag gcaacgggtc ggtgcgaagc gccgccccgt ttggctcctc gcaatgacga
     1141 tgtggcggtt attttccctt gaattgcggt ttgcgtttct cgccgaacgc cttgacgcct
     1201 tccctgaaat cgtcgctctc gcggacggca tcgagcagcc ggcgcacctc ggcgaccgtc
     1261 tccagcggtc ccttcggcat gacatcgcgc gcgagcttct tcagggactg cacgaccagc
     1321 ggcgcgttgg cggcgatctt cgccgccatt tcctgcgcct gcacgatatg ctgtcccttc
     1381 ggcgctatcc tgttcacaaa gccgatctga tacgcgcgct cgaccgacag ctcctcgccg
     1441 accagcagga attccatggc gatcttgtgc ggcatgcgcg ccaccaccga cgagatgccg
     1501 ccggcggtgg tcccgatttt tgcctcggga tacgtgaagc gcgtggtctc cgacgcgatg
     1561 cacatgtcgg ccatctgcac gagcacgaag ccgccgccga ccacccagcc ggaggttgcg
     1621 gcaatcaccg gcttgtccag ttcgacgccg agccccggca cggcgtgcca catgttcacc
     1681 gggaaatcct tcacgtcggc gccgaccgaa aaataggcgt cctcggcgga ggcaagcacc
     1741 gcaacgcgat cgtcgctgtc gcgaaaggtg agccaggcct ggcgcagctc ttcgcacagc
     1801 gcgccgttca gcgcgttatg tttctcgcga cgatccatgg taatggtggc gacgcgctcg
     1861 gcactctcgt agcggacaag ggtcatggat ttcctcacgc ggcgttctct tgattgttgt
     1921 tttccgtcat agcgagcaaa gcaagccggc atctgcccgc ggaaacagtc cggatcgcgt
     1981 cgtcgctcgc gcttctcgca acgacggcag cgaccgttat ttcttctcgt ccgggtgacg
     2041 gtgcaccgga tcgacccaca gcaccgtttc cggtttctcg accggctcga tatcgagatt
     2101 gatcgcgatc gcctcgccgt cgcttcgcac cagcacgcat tccagcactt cgtcgcggct
     2161 cgcgttgatc tcctgatgcg gcacataggg cggaacgtag atgaagtcgc cggggccggc
     2221 ttccgcggtg aattgcaggt gctcgcccca gcgcatccgt gccttgccct tcaccacata
     2281 gatgatgctt tcgagatggc cgtggtgatg cgcgccggtc ttggcgtcgg gcttgatggt
     2341 caccgtgccg gcccagagct tctgcgcgcc gacgcgggca aagttgatcg cggccttgcg
     2401 gtccatgccc ggcgtcgacg gcacg
//
LOCUS       DATLJT010001503         9750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_138251,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001503 DATLJT010000000
VERSION     DATLJT010001503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138251"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VM910_34385"
     CDS             complement(<1..1037)
                     /locus_tag="VM910_34385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67633.1"
                     /translation="MAILIRDYETRSTLDLPDVGAWRYSQHADTDIWCCGYCVDDGEI
                     KLWLPGDPVPAEFVEAAAKPDWLVCAFNDQFERLLEQHILGPRYGFPTIPIERHRCLQ
                     AAALARALPGSLDGAASALKLPQQKDVIGRRVMLQMAKPRKPRKGEDPKAILWFDDPG
                     RRQQLYAYCKQDVTTERELHRQVPFLDGAEQALWILDQAINDRGVYIDRQLLDAGLRI
                     AEQGRTEIDVELAKITEGEIDSVHQVARLLEWLNAHGAKLKSINKENLEKALTNGLPD
                     TTRQVIQLRLDGAHAATNKLYTMRSWLNGDDRARGTLKFHGASTGRWSSYGIQLQNLK
                     RPVVDDIDHAMA"
     gene            complement(1104..1775)
                     /locus_tag="VM910_34390"
     CDS             complement(1104..1775)
                     /locus_tag="VM910_34390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67634.1"
                     /translation="MTDDVHNLPAVQFDDEEGYDDRLIKGEIAKCVDARWSVRDGTDL
                     KDTEWFLVAGTTQALQRFTDNVPEFILKEPGKPLPDVDELNEQIPQDEWEEGLNGEPR
                     PPWQRVYVVYLIRLHDASILTFISGTAGARIAYNALTSKVYNMKVLRGTSVAPIVKLS
                     SKPMKTKFGTKMRPDFEVVEFREIGGSSVPQIESPKTGGGGEQIGKPVKPMSIQEELN
                     DNLPF"
     gene            complement(2198..2587)
                     /locus_tag="VM910_34395"
     CDS             complement(2198..2587)
                     /locus_tag="VM910_34395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67635.1"
                     /translation="MRTHTYTATASPVNSLAPWSIRTISARSIAKADWKPRRRALEAA
                     RWVAGHTLIKPVTATLASRVFGVSAQLISEALKQERGKHNGNGTAGSSILNQAWDQAT
                     PAERAALGHAVGVDAIWDAAVAPNLAP"
     gene            complement(2713..2919)
                     /locus_tag="VM910_34400"
     CDS             complement(2713..2919)
                     /locus_tag="VM910_34400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67636.1"
                     /translation="MSITSIGFEGRLDRVRSLKEFAAHVGLSFKTLKRLIAVGQGPRV
                     TQLSARRIGIRDSDGQAWLEGRSR"
     gene            complement(2997..3527)
                     /locus_tag="VM910_34405"
     CDS             complement(2997..3527)
                     /locus_tag="VM910_34405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67637.1"
                     /translation="MQPFKSKPDAATNPQVYWDAADAEFATEEDPEPSAQAFLISKLR
                     DGSLPFFVHDAAGKIVKGDSKLHLRTLAAIQRVLIGARDLERTLAEGSAAPQQVHRIS
                     NEQSAERFADGYIKSEKEAGRTPTQRGVEQAAKNAGIHGGRQLLRNAYNRLQRSAGIA
                     VERGHPKNSPNQFAKK"
     gene            complement(3741..4934)
                     /locus_tag="VM910_34410"
     CDS             complement(3741..4934)
                     /locus_tag="VM910_34410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI67638.1"
                     /translation="MRLTATSIKALALPPGTSDKTFFDDELHGFGVRIRSGGTARWVV
                     QYDIGGKTKRVTLGTTAMLNASVARDKAKDLLAAIRLGADPAAEKREARLRAVETFGA
                     LLPRYLAYQRARRRPRSYTQLERHLVKYARELHPRPLAAIDRRTIAKLISELTEESGA
                     ATAKVTRDSLSQYFTWLQREGVLEANPVTFTNVPTMNGARTRLLSDEELRKIWSALGD
                     GDYSDVVKLLIFTGARRLEIGDLSWHEVDLEAAEIRLPAERCKNKRPHTILLSEPALS
                     ILAKRPRNERGYVFGRGAFGFQGWSHCKEMLNARVPNVKDWVLHDLRRLISTAMHDRL
                     GVPPHVVEAVLGHVGHRSGVAGTYNKALYVDERRRALARWGEHVLGIVTGKAVKAKVV
                     NLRKR"
     gene            5390..5557
                     /locus_tag="VM910_34415"
     CDS             5390..5557
                     /locus_tag="VM910_34415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67639.1"
                     /translation="MKTLITALALAALIAATQSAAAEPGDRRDIGQPGQGYEGTYQGY
                     PLSDWYRTDCW"
     gene            5670..5873
                     /locus_tag="VM910_34420"
     CDS             5670..5873
                     /locus_tag="VM910_34420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67640.1"
                     /translation="MKTIVKTIATRYAMTVGLAGALALTAASYSFAQIRTGAEGRTTV
                     SQYCAPPDEESPDARRFYCRSGV"
     gene            6033..7001
                     /locus_tag="VM910_34425"
     CDS             6033..7001
                     /locus_tag="VM910_34425"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67641.1"
                     /translation="MPRFFIASFFAVVSLALCATAPAQDYPTRPLRLIIPLGAGGGGD
                     VFTRALADELSKALRQPVVVENRPGGALNIGTRACAEAAADGYTVCVLSSEPMVYNQF
                     AFKSLPFDPERDFEPISLLFFNTLALVTNRSLKVRTIPELVALAKAKPGTLSYGTFSF
                     PLAHFMEKLKQETGADIVRIPFRSGNEVVNAVLSGSTPVAILALSNMVPQLQSGHITA
                     LVVTGKTRSPLFPDIPTLAEVRGGENYPATWFGLFAPAGTPKPIIAHLAHEVARIVDD
                     RDFRQRMYIDRAVEPSGVRLEEFAHFIRDEREAAERMVRESGLQPK"
     gene            complement(7077..7634)
                     /locus_tag="VM910_34430"
     CDS             complement(7077..7634)
                     /locus_tag="VM910_34430"
                     /inference="COORDINATES: protein motif:HMM:NF040428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HVI67642.1"
                     /translation="MSERSRHFDMRVRKSMKSRKEPLVERRALPTPERLRRAGKDFER
                     GDTGQITMRDSPLERAFARNALTPEQYSAGQKYRHHWYHAGLADRLESIDLNRVFAID
                     VGAYSGMAKTENQLFHRQRYREAAQAVGKIGSHVLEWAVCREIALEQVGQTLGWSSRP
                     QAYAAAVERMKTALDELCKLWGIRS"
     gene            8389..8991
                     /locus_tag="VM910_34435"
     CDS             8389..8991
                     /locus_tag="VM910_34435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVI67643.1"
                     /translation="MAREKRFKSARLAGKAMGIPVSTYGAHERAQNPGGRDYGPEDAK
                     KYARRFGVTAEWLLTGREPFPTGADESPPAPKVQVVGYVGAGSAAYLYDVAQPDLDEV
                     GGPADAAEDTVAVEIRGDSLGPFFNRWYIFYDDVHRPMTADLIGQLCVVGLADGRVLV
                     KQVQRGRAEGLYNLHSATEKPIADVKIEWAARVNSISRRS"
     gene            9088..9405
                     /locus_tag="VM910_34440"
     CDS             9088..9405
                     /locus_tag="VM910_34440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67644.1"
                     /translation="MAAKHQDQTRTPEARRPADRASRFIARVDAHLPTLANDGDRRAF
                     LDRQLDGWEHRYACFIATDGASEFAASRADPPQAADFLLTIIGLAARRIALGRDAKAD
                     DHG"
BASE COUNT         1742 a         3042 c         3021 g         1945 t
ORIGIN      
        1 gccatcgcgt ggtcgatatc gtcaacgaca ggcctcttga ggttctgtag ctgaatcccg
       61 taggacgacc agcgtccggt cgaggcgcca tgaaacttca aggtgccgcg ggcgcggtca
      121 tctccgttga gccacgagcg catggtatag agcttgtttg tcgcggcgtg agcgccatcc
      181 aagcgaagct gaatcacctg cctggtcgtg tccggcagcc cgttggtcaa agccttttcc
      241 aggttttctt tgttgatgct cttgagcttg gcgccgtggg cgttgagcca ttccaatagc
      301 cgtgcgacct ggtgcacgct gtcgatctca ccctcggtga ttttcgccag ctcgacatcg
      361 atctcggtgc gcccctgttc tgcgattcgc aatccagcgt cgagaagctg gcgatcgata
      421 tagacgccac ggtcattgat cgcctggtcg agaatccata gcgcctgctc ggcgccgtcg
      481 aggaagggta cttggcggtg caattcgcgc tcggtggtca cgtcctgctt gcaataggcg
      541 tagagctgct gccggcgccc agggtcgtcg aaccagagga tggccttagg gtcttcaccc
      601 ttccgcggct tcctcggctt ggccatctgc agcatgacgc gccggccgat gacgtctttt
      661 tgttgtggca gtttgagtgc gctggcggca ccgtcgagac tgccgggaag ggcgcgggct
      721 aacgctgcgg cctgcaggca ccgatgacgt tctatcggaa tcgtcgggaa gccgtaacgc
      781 ggacccagga tatgctgctc aagcagccgc tcgaattggt cattgaaggc gcagaccagc
      841 caatcgggct tagcggctgc ttctacaaat tctgcgggaa cgggatcgcc gggaagccaa
      901 agttttattt caccatcatc aacacaatag ccgcagcacc agatgtcggt gtcggcatgc
      961 tgcgagtagc gccaggcgcc cacatcaggc aggtcgagcg tggatcgcgt ctcataatcg
     1021 cgaataagaa tggccatgac ggacccgaga agggagaagc tcggctgccg ggaaacagcc
     1081 gagccaaggg tgagcgcatg acgttagaac ggcaggttgt cgttcaactc ttcttggatc
     1141 gacatcggct tcaccggctt gccgatctgc tctccgcctc caccagtctt tggagactcg
     1201 atctgcggca ctgagctacc gccgatctca cggaactcga caacctcaaa gtccggcctc
     1261 atcttggtgc cgaacttggt cttcatcggc ttgctggaaa gcttgacgat cggcgcgacg
     1321 ctggtgccgc gcagaacctt catgttgtag actttgctgg tcagcgcgtt gtatgcgatc
     1381 cgtgcaccag cggtgccact gatgaaggtc aggatcgagg catcgtgcag gcggatcaga
     1441 tagaccacgt atacacgttg ccagggcgga cgtggctcgc cgttgagccc ctcttcccat
     1501 tcgtcctggg ggatttgttc attcagctcg tccacatcgg gcagcggctt gccaggttct
     1561 ttcagaatga actcgggcac gttgtcggtg aaacgctgca gcgcttgagt ggtgccagcg
     1621 acaagaaacc actcagtatc tttcaaatcg gtgccgtcgc gtacactcca gcgggcatcg
     1681 acacatttcg cgatttcacc cttgatcagt cgatcgtcgt agccctcctc atcatcgaat
     1741 tgaacagcag gcagattgtg aacatcatca gtcatgggga tgcctcattg tttccctgtt
     1801 tcgccggatt tgagtgcgtc tcggccgcat tttcgcatgg tgcgaaaact gagaatttcg
     1861 acttcacacg tgctcctctg aggcttgctc acgctcaatt tcgatcacac ggaagcggaa
     1921 ccgacggagc atcagtttca acatgatcct taggcggcgg atgtcatcgt ggtcggaacc
     1981 gatcgaccgc aggcgcattc ggtaaatctt cgtgggaggt ttcaccccca cgcgactctc
     2041 ccttctgctg cttgggttgt gctgataaac cgcggtcatg gatccaggca ttccgttcaa
     2101 attcttcggc gggttcgtca ccgatctact tctctccccg gctctcgtgg gagccgggct
     2161 gcgatcgctc acagcccggc gccgcacgcg cagtccttca gggagcaagg ttcggggcga
     2221 ccgcggcgtc ccatatggcg tccaccccga cggcgtggcc aagcgctgct cgctcggccg
     2281 gcgttgcctg gtcccacgcc tgattcagaa tcgaagagcc tgctgtcccg ttgccgttgt
     2341 gcttaccgcg ctcctgcttg agcgcttcgg agatcagctg cgccgaaacg ccgaacactc
     2401 tggaggcgag tgtggccgtc accggcttaa tcagggtgtg accggcgacc cagcgggcgg
     2461 cttcaagtgc tcggcgccga ggtttccagt ccgcctttgc gatcgatcga gcggagatcg
     2521 tcctgatcga ccagggcgcg agggaattga cgggtgaggc cgtcgcagtg taggtgtgag
     2581 tacgcacttt agatgtctcc ttcttctaac ggcctcggcg gtgatgcgct tgaggccgtt
     2641 tgccttgccg cccgtgttga aaccgcgatc cgacgccgaa cggcggcacg ccggagcggg
     2701 agcttgtcgc gtttaacgcg atcgcccctc gagccacgct tgtccgtcgc tgtcacgaat
     2761 gccgatgcgg cgcgcagaca gctgcgtcac gcgcggtccc tggccgacgg cgatgagtcg
     2821 cttcagggtc ttgaaactca atccgacgtg cgcggcgaac tcttttagtg accgcacccg
     2881 atcgagcctg ccttcgaaac cgatgctggt gatggacata agcgctccaa ctggggtcgt
     2941 tggagcgaca agagcacaga cgcgaaagga gagccctatt tcgcaattcg ccgcaattat
     3001 tttttggcga attgattcgg cgaattcttt ggatggcccc gttccaccgc aatgccagcc
     3061 gaacgctgca ggcggttata ggcattgcgc agcagctgcc ggcctccatg aattccggcg
     3121 ttcttcgcag cctgctcgac tccgcgctgt gtcggcgtgc gtcctgcttc tttttcgctt
     3181 ttgatgtagc cgtccgcgaa acgctcggct gactgttcgt tactgattcg gtgaacttgt
     3241 tgtggcgccg cactcccctc agcgagtgtg cgttcgagat cgcgggcacc aatcagcaca
     3301 cgctggatcg ccgctagtgt acgcaaatgc aacttagaat cccctttaac gatcttgccg
     3361 gcagcgtcgt gcacgaagaa aggcaaactc ccatcacgaa gcttgctaat cagaaacgct
     3421 tgcgccgacg gctcaggatc ttcttccgtt gcgaattcgg catccgcagc atcccagtaa
     3481 acctgaggat tcgttgcagc gtcaggtttg cttttgaagg gttgcaagcg ttcgtcccga
     3541 cgctttttcc attctgcgtg cagggcggct acggcctgct cgaccggagg gtgcgcttgt
     3601 tcgaacgtta gtagggtttg tcttgtggct tgtggtcgcc gcgaccggcg actatttttg
     3661 gtgggcattc cgcacctgtg ccttaggtgg tggattgagg cccggcggca gttttgaacg
     3721 gctccgccgg gcctcgcttt tcaacgtttc cgcagattca cgaccttagc cttcaccgcc
     3781 tttccggtca cgatcccgag cacgtgttcg ccccagcgcg ccagcgcgcg acggcgctcg
     3841 tcgacataga gcgccttgtt gtaggtgcca gcgaccccgc tgcggtgccc gacatgcccg
     3901 agcacggcct cgaccacgtg tgggggcacg ccgagtcggt catgcatcgc agtcgagatg
     3961 agccgtctca gatcatgcag gacccaatcc ttcacgttcg ggacgcgcgc gttgagcatc
     4021 tccttgcagt gcgaccagcc ttggaaaccg aaggcgccac ggccgaacac atagccccgc
     4081 tcgttgcgcg gccgctttgc caggatggag agcgcaggct ccgacaacag gatcgtgtgc
     4141 gggcgcttgt tcttgcagcg ctctgcaggc agacgaatct cggctgcctc caggtccacc
     4201 tcgtgccatg acaggtcgcc gatctcgagc ctccgcgcgc cggtgaagat gagcagctta
     4261 acgacatcgg agtagtcgcc gtcacccagc gcggaccaga tcttgcgcag ctcctcgtcg
     4321 ctgagcaggc gcgtgcgtgc gccattcatg gtggggacat tggtgaaggt gaccgggttg
     4381 gcttcgagca cgccctcgcg ctgcagccac gtgaaatatt gactcagact atcgcgcgtg
     4441 accttcgcag tcgcggcccc gctctcctcc gtcagctccg agatcagctt ggcgatggtg
     4501 cggcgatcga tggcggcgag cgggcgggga tggagctcgc gtgcatattt caccaagtgg
     4561 cgttccagct gcgtgtagga gcgcggtcgt ctgcgagccc gctgataggc gaggtagcgt
     4621 ggcagcagcg cgccgaaggt ttcgaccgca cgcaagcgtg cctctcgctt ctccgctgct
     4681 ggatcggcgc cgagtcggat ggcggcgagc aggtccttgg ccttgtcgcg cgctacgctc
     4741 gcgttgagca tggcggtggt gccgagcgtt acgcgcttgg tcttgccgcc gatgtcgtac
     4801 tgcaccaccc agcgcgccgt gccgccgctg cgtatgcgga cgccgaagcc gtggagctcg
     4861 tcgtcgaaga acgtcttgtc gctggtgccg ggcggcagtg cgagcgcctt gatgctggtc
     4921 gctgtgagcc tcattcgatt cctctctggg caggccatgg gcaggttggg cgtgtccctt
     4981 ggcggcccct cttggccctc tcgtgacctg gttatatagc gggtatactc gcaaggctgg
     5041 gaggttacaa gcgacagacg aggtccacgc ggggtcacct ggggtcaccg gaaactagcg
     5101 gcgctatcgg gagtaaagcg agcatcattt gcccgtttcg agcatttccg cgcttgaccc
     5161 aaagccgact tgaccgtgaa gcttgggcca gagcgctgta aaaattgggc taggtctctg
     5221 aaattgctga attgatacat atagttctga atcgtctcca agtgtgactt gcatcacagg
     5281 actgtgacac cgccggtggc atcctccatt tcgcaggcgc cggcctgtcg gcccatgcgc
     5341 tgctgcacga accgacggac gatggtccgt cacacggatg gaagcaacca tgaagacact
     5401 catcaccgcg ctggctctgg ccgccttgat tgcggccacc cagtccgcag cggcagaacc
     5461 cggcgatcgc cgcgacatcg gtcagccggg acaaggctat gaaggtacct atcaggggta
     5521 ccccctgagc gattggtaca ggactgactg ctggtaaagt caaatcagat cgccacgaag
     5581 gcgtcggggc ttcgcagcga gccgctcttg ggcggccggc tcgcgggccg gccgcgcaac
     5641 ctcgaaacac tcgagcagga ggtgcggcca tgaaaacgat cgtgaagacg atagcgacac
     5701 gctatgcgat gacagtcgga ctggccggcg cgctggcgct gaccgcggcg agctactcgt
     5761 tcgctcaaat tcgaacggga gcagaggggc gcaccaccgt gtcccagtat tgcgcgccgc
     5821 cggacgagga gagccccgac gcccgcaggt tctattgtcg cagcggggta tgactgcgca
     5881 gcggcttttc cgcctacggt gcaccgccgc cggcctgcct aagccgcgcg cgcaagcaaa
     5941 tcgatacgcc cgcgtgacgc gttgccatcc gctttgcgcc gcgatggccg gcggcctatc
     6001 ctcaacaaaa ataaagaaca tggagggagg tcgtgccgcg attttttatc gcgtcgttct
     6061 tcgccgtcgt ttcgctcgcc ctgtgtgcga ccgcgccggc ccaggactat cccacccgac
     6121 ccctcaggct catcatccct ctgggcgccg gcggaggggg agacgtgttc acgcgagcgc
     6181 tcgccgacga gctgtcgaag gcgttgcgcc agccggttgt ggtggagaac aggcccggcg
     6241 gcgcgctgaa catcggcacg cgggcgtgcg ccgaagcggc tgcggacggc tacaccgtct
     6301 gcgtcctttc gagcgagccg atggtctaca atcaattcgc gttcaagagc cttccgttcg
     6361 atcccgagag ggatttcgag ccgatcagcc ttttgttttt caacacgctg gcgctggtca
     6421 ccaaccgctc cctcaaggtg cggacaattc cggagctggt ggcgttggcg aaggccaagc
     6481 ccggcacgct gagctacggc acgttttcgt ttccgctcgc gcatttcatg gagaagctca
     6541 agcaggagac cggcgccgat atcgtgcgca tcccgttcag gagcggcaac gaggtggtca
     6601 acgcggtgct gtccgggtcg accccggttg cgattctggc gctctccaac atggtcccac
     6661 agctgcaaag cggccacatc accgccctcg ttgtcaccgg caaaacccgc tcgccgcttt
     6721 ttcccgatat tcccacgctg gcggaggtcc gcggcggcga aaattatccc gcgacgtggt
     6781 tcggcttgtt cgcgccggcc gggacgccga agccgatcat cgcccacctc gcgcatgagg
     6841 ttgcccgcat cgtcgatgat cgcgacttcc ggcaacgaat gtacatcgat cgtgccgtcg
     6901 aaccgtcggg ggtcagattg gaggaattcg cgcacttcat tcgcgacgag cgcgaggccg
     6961 ccgaacggat ggtcagagag tcgggactgc agccgaaata agcgtgcgga attcgagctc
     7021 gagccgcagg agcagcatcc gtgcgaagct ggagtcggcc tggcccacgc agagtctcag
     7081 ctcctaatcc cccacagctt gcacagctca tccaaagccg ttttcatccg ttcgacagca
     7141 gccgcatagg cttgcggccg actgctccag cccaatgtct ggccgacctg ctccagcgca
     7201 atttcccggc acaccgccca ttccaatacg tgcgatccga tcttgccgac ggcttgcgcg
     7261 gcctcccggt atctttgccg atggaagagt tggttttccg tcttcgccat ccccgaatag
     7321 gcgccgacgt cgatggcaaa cacgcgattg agatcaatcg attccaatcg gtcggcaagg
     7381 ccggcgtgat accagtgatg gcgatatttc tgcccggctg aatattgctc tggcgtgaga
     7441 gcgttgcgcg cgaaagcgcg ttccaatggg ctgtcccgca tggtgatctg gccggtgtcg
     7501 ccgcgctcga aatccttccc ggcgcgacga agccgttccg gcgttggcag cgcgcgccgc
     7561 tcgaccagcg gctcctttcg gctcttcatc gatttccgta cccgcatgtc gaagtgtctt
     7621 gatcgctcgc tcattttttc tctccggtcg atcggcgtag actggcagtt tcgctgctag
     7681 gcatgaggga cctatctgcg tcgcgggtgg cgtgccgcgg gcgcgacgtc gtgtcgactc
     7741 cccccgccgc cgataggcgc gcgcggagtg ctcggcgcag tacggcttgt tgcgaagcgg
     7801 ttgcgctcca cagaagaaga agcgagagga cctcggatcg ccgaccggcc aacggcaggt
     7861 atgctcgctc aattcgagca gcgagcggcg ctggcggcgc gggatacgcg cgtccacacc
     7921 cttagtctcg acatagggct tggcgttgaa gacccaggcc ggcggcggtc ctttccggaa
     7981 ccaccaggcg gcgcgctgtg cgtgtacggg cctatcgccg ggacgcgtgt tggctgtgta
     8041 gcgtctgccg ggcgcgccgc cgtaaggcga aagcgtggaa atcccgaggc gatggatctt
     8101 ggagatgacc gcgttgcagg tgatgccatg acccaactcc gcggcgatct cgcgcacgcg
     8161 ggcgcctcgt cgccagcgtc gccgcaacag ccggatgcgc tctgccgtcc acggcgaggc
     8221 gcgccgctgc ggcgttggca gaacccctct cgataatggc aacatcacgc ctccggatca
     8281 ttcttggaag ggacttgact acaatttgtc tccgatgtca accaccaaac gtggttataa
     8341 tacaagtagt attcgcctat ggctacgact cgcggagagc ggctacgcat ggcgcgcgag
     8401 aagcgcttca agtcggcgcg ccttgccggc aaggcgatgg gcattccggt gtcgacctac
     8461 ggcgcgcacg agcgagcgca aaatcccggc ggccgagact atggcccgga ggatgcgaaa
     8521 aaatatgcgc ggcgcttcgg ggttacggcg gaatggctgc tgaccggccg cgagccgttc
     8581 ccaacgggcg cagacgagtc gccaccggcg cccaaggtcc aggtggtcgg ctatgtcggg
     8641 gccggctcgg cggcgtatct ttacgacgtg gcgcaaccag atctcgacga agtgggcggc
     8701 ccggccgacg ccgccgagga cacggtcgcg gtcgaaatcc gcggcgacag tctggggccg
     8761 ttcttcaacc gctggtacat tttctacgac gatgtgcacc gtccgatgac ggccgacctg
     8821 atcggccagc tctgcgtcgt cggtctcgcc gacggccgtg tcctcgtcaa acaggtgcag
     8881 cgcggccgcg ccgagggcct gtacaacctg cattccgcca ccgagaagcc catcgccgac
     8941 gtcaagatcg aatgggcagc gcgggtaaac agcatttccc gtcgcagctg aacttaatcg
     9001 aaattactgc aaaatgtagt tgacaagatc tgctacataa tgtagccatc ttctccacag
     9061 cctgaggagg gactgtcgga gaacaagatg gcggcgaaac accaagacca aacgcggacg
     9121 ccggaagccc gccgccccgc cgatcgtgca agccgcttca tcgcgcgcgt cgacgcccac
     9181 ctgccgactc tggcgaatga cggggaccgc cgtgccttcc tcgaccgcca gctcgacgga
     9241 tgggagcacc gctacgcctg cttcatcgcg acggacggcg cgtcagagtt cgccgccagt
     9301 cgtgccgatc cgccgcaggc cgccgatttc ctgctcacca tcatcggcct cgcggcgcgg
     9361 cgcatcgccc tcggccggga tgcaaaggcg gacgatcatg gctgacgaca acgggtccct
     9421 cagcagcgcg attctgtcgc tcttggtcgc tgccgaccag cggtgtcccg caatcatcgg
     9481 gcaagcgcat cttctctacc acgctcgcgc gaacgatccg ccagagaatg ccgcgaaaac
     9541 actcacgcaa ttgaagcggg acgctgatga tctcactcga gccatcgcca acgtcgaggc
     9601 cgccatgaaa cgagccgggt cggacccgac ataactatct cgcggtcggt cagaccaatc
     9661 cggttcgcgc accaagcgtg gtatggagga cattgcgcga cgggttcaca gcatcaaatc
     9721 cttgttctcg aggccgaaca tcacgccgcc
//
LOCUS       DATLJT010001504         1533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_826510,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001504 DATLJT010000000
VERSION     DATLJT010001504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826510"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VM910_34445"
     CDS             complement(<1..523)
                     /locus_tag="VM910_34445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HVI67645.1"
                     /translation="MAKSVYRRVVVKVSGEALMGPDSFGIHQPTLERIAADLMASQRL
                     GVAIAVVVGGGNIFRGVNVSEKGVARATGDMMGMLATVMNALALESAIEKAGLAARTM
                     SALTMPDVCEAYERGRALRHLEQGRLVLLAGGTGNPFFTTDTTAVLRAAEIGAEAVLK
                     ATNVDGVYSADPKK"
     gene            complement(606..1529)
                     /gene="tsf"
                     /locus_tag="VM910_34450"
     CDS             complement(606..1529)
                     /gene="tsf"
                     /locus_tag="VM910_34450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HVI67646.1"
                     /translation="MANITAQMVKELRERTGAGMMDCKGALTETGGEMEQAVDLLRKR
                     GLAKAAKKAGRIAAEGLIGLAVDGSQGVLVEVNSETDFVARNELFQRLVKTIANVALT
                     VGSDVETIKAAKMGDITVADCIAESIAKIGENISLRRASSLSVANGVVGSYVHNSVDE
                     GLGKIGVIVALESTGTGDELKRFGRMLAMHVAASNPQAVDPSGLDPDVVRREKEVLAD
                     KYKAQGKPANVIDKIVESGLKTFYKEVCLVDQAFIHDPDKSVSQAIKDVEGKVGAPIK
                     VTGFVRYVLGEGIDRPDGDFGGDVAALIKPN"
BASE COUNT          264 a          532 c          436 g          301 t
ORIGIN      
        1 ccttcttcgg atcggcgctg tagactccgt cgacattggt ggccttgagc accgcctcgg
       61 cgccgatttc ggccgcccgc agcacggcgg tggtatcggt ggtgaagaac ggattgccgg
      121 tcccgcccgc gagcaggacc agccgcccct gctcgagatg gcgcagcgca cgcccgcgct
      181 catacgcctc gcagacgtcg ggcatggtga gcgccgacat ggtacgagcc gccaatcccg
      241 ccttttcgat cgcgctttcc agcgccagcg cattcatgac ggtcgcgagc atgcccatca
      301 tgtcgccggt cgcacgcgca acgcctttct ccgacacgtt cacgccgcga aagatattgc
      361 cgcccccgac cacaacagca atcgccacgc ccagccgctg ggaggccatc agatcggccg
      421 cgatccgttc caacgtcggc tgatggatgc cgaagctgtc gggacccatg agagcctcgc
      481 cggaaacctt caccacgacg cgccggtaga ccgattttgc catggcactc ctgcgctttg
      541 ccgccggcgc gagcggctcc acacggctat cgcatgggtc gaacgctcaa taccgcttgg
      601 acgactcagt tcggtttgat caaggccgcg acatcgcccc cgaaatcacc atccggccgg
      661 tcgatacctt cgccgagcac gtagcgcaca aaccccgtca ccttgatcgg cgcgccgacc
      721 ttgccctcca cgtccttgat cgcctgtgaa acgctcttgt ccggatcgtg gataaaggct
      781 tgatcgacca ggcacacctc cttgtagaag gtcttgagcc ctgattcgac gatcttgtcg
      841 atcacattcg caggttttcc ctgcgctttg tacttgtcgg caaggacctc cttctctcta
      901 cgtacgacat ccgggtcgag gccggaggga tcgaccgcct gcggattgga ggccgcaaca
      961 tgcatcgcaa gcattcggcc gaaacgcttg agctcgtcgc ccgtgccggt cgattccagc
     1021 gccacaatga ctccgatctt gccgaggcct tcgtcgacgg aattgtgcac gtagctgccg
     1081 acgacgccgt ttgcgaccga caaggaggat gcccgtcgca gcgatatgtt ctcaccgatc
     1141 ttggcgatgc tctcggcaat gcaatcggcc acggtgatgt cgcccatttt cgccgcctta
     1201 atcgtctcga catcactacc gacggtcagc gcgacattcg cgatcgtctt caccaggcgc
     1261 tggaacaatt cgttgcgggc gacgaaatcg gtctcggaat tgacctcgac caagacgccc
     1321 tgcgaaccat cgacggcgag tccgatgagg ccttcggcgg cgatgcggcc ggccttcttg
     1381 gcggccttgg cgagcccgcg cttgcgcagc aaatcgacgg cctgctccat ctcgccgccg
     1441 gtttcggtca gcgccccctt gcagtccatc atgcctgcgc cggtgcgttc gcgcagctcc
     1501 ttcaccattt gcgccgtgat gttggccatg gct
//
LOCUS       DATLJT010001505         1896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_369443,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001505 DATLJT010000000
VERSION     DATLJT010001505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369443"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            515..1225
                     /locus_tag="VM910_34455"
     CDS             515..1225
                     /locus_tag="VM910_34455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI67647.1"
                     /translation="MTDIDILPVQRLHFQVFSLPWPFAADRRTEIEDYFTHLRREKPQ
                     LWNGRVLLLREFEISGATLQGTMFETDYASFIAWRDWGFPDRDVHACFAMGALLSRDG
                     AFLLGVMNAHTAGAGGIYFPSGVLDPEDVVCGRVDFERNVRREIAEETGLSLEDVTAD
                     PWLLVLSQPRVALIKVFRFRQSASEMRASILDHLQKETLSELSDVHIVRDPSDFKPSM
                     PPFVSAFLLHFWACERER"
     gene            1310..1531
                     /locus_tag="VM910_34460"
     CDS             1310..1531
                     /locus_tag="VM910_34460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67648.1"
                     /translation="MALVVVSIYLASVVFCVGTTAWILRAWTRRRYWHKSPSDLHYVV
                     AGTALLLSAGIGVAVASQIVLALGYPPAP"
     gene            1648..>1896
                     /locus_tag="VM910_34465"
     CDS             1648..>1896
                     /locus_tag="VM910_34465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67649.1"
                     /translation="MSELVQFLCKGDHPIEARLREKTRDALKESIASGHVRIKFTDTR
                     GGTELGVAIDRSRSDLRAIESDNGSAEIRVVGDLTLDYV"
BASE COUNT          349 a          543 c          586 g          418 t
ORIGIN      
        1 gatgatttcg cgcatcgacg aggcgcattg ttcggcagtt ttcaggtctt caaagattat
       61 cgtcagcgag ggagaccggc cgtcttgttg gatcgtgaac ccgggactgg gaatgaagcc
      121 cgacccgatg tcgatgttct ctaccttccc cactctccaa atcattcgat tgctccgtca
      181 agtcgatcca attctcgcct cggcccgttg ccttgcagcc gagcgcagtc cgtgatctcg
      241 agcggctcgc aagcctgtct tggatcgaag ccgaggtcat gcgcctcgtg tcgaacgtcg
      301 ctgcgccgac tgagccgagg caccctgggg gtcgctggtc gatgggttga tcgtcgcaac
      361 atgaaactcg catcgatcac ggttttcggt caaatgcgat aggatcggcg tccgaggacg
      421 ttacacggga aaggttcttc cgagcttcga gcgggcgctg accaagccgg cggcgcattc
      481 ctgcggagca gcctgccagc gccgaaagtg caaaatgacg gacatcgata tccttccggt
      541 tcaacgcctg cattttcaag ttttttccct gccatggcca ttcgctgcgg accgccgcac
      601 cgagatcgaa gactatttta cgcatttacg gcgcgaaaag ccccaattgt ggaacggacg
      661 tgtcctcctc ctgcgcgagt ttgagatttc gggcgccacg ctgcagggca ctatgttcga
      721 gactgattac gcaagtttca tcgcctggcg cgactggggc tttcccgacc gggacgtcca
      781 cgcgtgcttc gcgatggggg cgttgctatc ccgcgatggt gcgttcttgt tgggcgtgat
      841 gaatgcccat accgccggtg ccggcggcat ttactttccg tccggcgtgc tcgatccgga
      901 agatgtcgtc tgcggccgtg tcgatttcga acgcaacgtc cggcgggaga ttgccgagga
      961 gaccggtctc agccttgagg atgttacagc tgatccctgg ctccttgtgc tctcgcagcc
     1021 gcgagtcgcg ctcatcaagg tattccgctt ccgccagtcg gcgagcgaga tgcgcgcgag
     1081 cattctcgat catctgcaaa aagaaaccct atccgaactt tccgacgttc acattgttcg
     1141 cgacccctcc gatttcaaac cgagcatgcc tccattcgtg agcgcatttc ttctgcattt
     1201 ttgggcttgc gagcgtgagc gatgacgtcg gtgtctgctc ggggcgacgg catggccgcg
     1261 gctttcgctg cgatgtaaca tctgagcaga ttcgcctggt ctgcgcggca tggcccttgt
     1321 ggttgtttcg atctatctcg cctccgttgt cttctgcgtg gggacgacgg cttggatttt
     1381 gcgagcgtgg acccgcagac ggtattggca taagagcccg agcgatctgc attacgtggt
     1441 cgcggggacg gcgctgctgc tgagcgcggg aattggagtg gcggtcgcgt cacagatcgt
     1501 gctggcgctc ggctatcccc cagcaccctg agggcttgag ctaccgcgca ggatcaaggt
     1561 aggttgaccg cggagcccga atggcgccaa ttggcgcgag caggaacgaa cattggcgtc
     1621 attgcaagat caacgaggag gggtggagtg agcgagctgg tgcaatttct ctgcaagggc
     1681 gatcatccga tcgaagcgag gctgcgcgaa aaaacgcgcg acgcgttgaa ggaatccatc
     1741 gcgagcggtc atgttcgcat caaattcacc gacacgcgag gaggcaccga actcggcgtc
     1801 gcgatcgacc gcagccgctc cgacttgcgc gccatcgaaa gcgacaatgg gtctgcggag
     1861 atcagagtag tgggagacct gacgctcgat tatgtt
//
LOCUS       DATLJT010001506         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1978877,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001506 DATLJT010000000
VERSION     DATLJT010001506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978877"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="VM910_34470"
     CDS             complement(<1..2273)
                     /locus_tag="VM910_34470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI67650.1"
                     /translation="MNASSWFGTGGDNTLEALWKDGERVFCRRWENGAEGCRPAVLAV
                     LPAAEHPTPDSLTRLTHEYGLKDELDSTWAVRPLELVRERGQTMLMLEDPGGEPLARL
                     LGGPMEMGDFLRLAVGIAAALGKLHQRGLVHKDLKPAHILVHCADGQVRLTGFGLTSR
                     LPRERQAPEPPETIAGTLAYMAPEQTGRMNRSIDSRSDLYAFGVTLYQMLTGVLPFTA
                     ADPMEWVHCHIARKPVPPCELVETVPVAVSHIVMKLLAKTAEERYQTAAGAEHDLQHC
                     LADWERRHRVDAFPLGEHDTPDRLLIPEKLYGREREVDTLVATFDRIVASGPPELVLV
                     SGYSGIGKSSVVNELHKVLVPPRGLFASGKFDQYKRDIPYSTLVQAFQGLVRPLLGKP
                     DTELASWRDALLEALEPNARLMTDLIPELKLIIGEPPPVPTLEPQQAQRRFMLVFRRF
                     ISVFARAEHPLALFLDDLQWLDSATLDLLEDLLTHPDLRHLMLVGAYRDNEVDATHPL
                     TRKLQAIRNAGGRIDEITLAPLACEHIGQLIAEALHCEPAHSAPLAQLVHDKTGGNPF
                     FVIQFLHALVEEDLLTFDHDARQWCWDPDRIHAKGYTDNVVDLMVGKLSRLPAGTRHA
                     LQQLACLGNVAGITTLSTVLGIPEAQVHAALWEAMRQELVERLESSYRFVHDRVHEAA
                     YSLIPEASRTSAHLRTGRLLAKAMPPEKREEVIFEIVGQLNRGAALITEQEEREQLAG
                     FNLLAGQRAKASTAYASA"
BASE COUNT          406 a          730 c          762 g          403 t
ORIGIN      
        1 gccgaggcat aggctgtcga cgccttggcg cgctggccag cgagcaggtt gaacccggcg
       61 agctgctccc gttcctcttg ttcggtgatc agcgcggcgc cgcgattgag ctgaccgacg
      121 atctcgaaga tcacctcctc ccgtttttcc gggggcatcg ctttggcgag tagccgcccg
      181 gtccgcagat gggccgaggt ccgcgaggct tcggggatca gtgaataggc ggcttcatga
      241 acacggtcgt gcacaaacct gtaagagctc tccagccgct cgaccagttc ctgacgcatc
      301 gcctcccaca acgccgcgtg gacctgtgcc tccggaatgc caagaacggt cgacagtgtc
      361 gtgatgccgg cgacgttccc gagacaggcg agctgctgca acgcgtgccg cgtcccagcc
      421 ggcagccggc ttagcttgcc gaccatcagg tccacgacgt tgtcggtgta acccttggca
      481 tggatgcgat cgggatccca gcaccactgc cgcgcgtcat gatcaaaggt gagcaaatct
      541 tcttcgacaa gcgcgtgcag aaactggatg acgaagaacg gattgccacc cgtcttgtca
      601 tggaccagtt gcgccagggg ggcgctgtgc gcaggctcgc agtgaagcgc ctccgcgatc
      661 aactggccga tatgctcgca ggcgagggga gccagtgtga tttcatcgat gcggccgccg
      721 gcgttcctga tggcctggag cttgcgggtc agcggatgcg tggcatcgac ttcgttgtca
      781 cggtaggcgc cgaccagcat caggtgccgc agatccgggt gggtcagcag gtcctcgagc
      841 aggtcgagcg tcgcagagtc gagccactgc aagtcgtcga gaaagagcgc aagcggatgt
      901 tccgctcgcg caaatacact gatgaaacgc cggaacacga gcatgaaacg gcgttgcgcc
      961 tgttgcggct caagggtcgg gacgggtggc ggctcaccga tgatcagctt cagttcgggg
     1021 atgaggtccg tcatgagccg cgcattgggc tccagcgcct ccagcaaggc gtctcgccag
     1081 ctcgccagct ccgtgtcggg cttgccgagc agcgggcgca ccaggccttg aaatgcctgg
     1141 accagcgtcg aataggggat gtcgcgcttg tactgatcaa acttgcccga cgcgaagagc
     1201 cctcgcggcg gcaccagcac cttgtgcagt tcattgacga ccgaggattt gccgatgccg
     1261 gagtagccgg agaccagcac cagctcaggc gggccgctcg caacgatccg atcgaacgtg
     1321 gcgaccaggg tgtcgacctc gcgctcccgc ccatacagct tctcgggtat cagcaaccgg
     1381 tcgggcgtgt cgtgttcgcc cagtgggaag gcatcgaccc ggtgccgacg ctcccagtcg
     1441 gcgaggcaat gctgcagatc atgctcagca ccggcggccg tctggtaacg ctcttcggcc
     1501 gtcttggcga gcagcttcat gacgatgtgg gagaccgcga cagggacggt ctccaccagt
     1561 tcgcacggcg gcaccggctt tctggcgata tggcagtgca cccattccat ggggtcagcg
     1621 gccgtaaacg gcagcacgcc cgtcagcatc tggtagagcg tgacgccgaa cgcatagagg
     1681 tcgctgcgcg aatcgatcga gcggttcatc cggccggtct gctcgggggc catgtaggcg
     1741 agcgtgcccg cgatggtctc gggcggctcg ggcgcctgcc gctcgcgcgg caggcgcgag
     1801 gtgaggccga atccggtgag ccggacctgt ccgtcggcgc aatgtacgag gatatgcgcg
     1861 ggcttgagat ccttatggac gaggccgcgc tggtgaagct tgcctagcgc cgcagcgatg
     1921 ccgacagcga gccgcaaaaa atcgcccatc tccatgggcc caccgagcag ccgcgcgagc
     1981 ggctcgccac cggggtcctc gagcatcagc atggtctgcc cgcgctcgcg cacgagctcc
     2041 agcggccgca ccgcccacgt gctgtcgagc tcgtccttta gcccgtattc gtgtgtaaga
     2101 cgtgtgaggc tgtcaggcgt cgggtgctcg gcggcgggaa gcacggccag aacggcgggt
     2161 cggcaccctt cggcgccatt ttcccacctt ctgcagaaga cgcgttcacc gtccttccag
     2221 agcgcctcta aggtgttatc cccaccagtg ccgaaccaag aggatgcatt catctgccgg
     2281 aattcccccg tcaggtcgtg a
//
LOCUS       DATLJT010001507         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4047288,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001507 DATLJT010000000
VERSION     DATLJT010001507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4047288"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VM910_34475"
     CDS             <1..168
                     /locus_tag="VM910_34475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI67651.1"
                     /translation="ACETAVLTLGGMGYSKEFHVERYLREVMIPRIAPITPHMILNFI
                     AEKILGLPKSY"
     gene            265..1161
                     /locus_tag="VM910_34480"
     CDS             265..1161
                     /locus_tag="VM910_34480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVI67652.1"
                     /translation="MTLSRRMAPVIALALLLGQSAWHCAWAEGPGGPAKKLARSACDH
                     AAFRVVVDVGHTAESPGAPSARGFWEYDFNLRLATLIKQKLLAAGFAKTVLLVTDGPA
                     RKSLFARAARANQLSADLFLSIHHDSVPDSFLERWEYEGHEHGFSDRFKGHSIFFSNE
                     NVDPDASLMFGQLLGEQLQARGLQYTAHYIEKFMGHRQRLLVDAKVGVYRYDQLIVLR
                     TTQMPAVLLEAGSIINRAEELVMNSPERQSLIGAAVTDAVEGFCAIRRPRNPDRVAGP
                     PRAAAKLAPDVAPQPASPAGAQ"
     gene            1372..2376
                     /locus_tag="VM910_34485"
     CDS             1372..2376
                     /locus_tag="VM910_34485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI67653.1"
                     /translation="MSIPKPVYNPPFNVTRASHSVLTVKDLAVSRNFYVDLLGFIVSD
                     EDSDALYLRGVAEACHHSLVLKRARGEPQAERVGMRVFSDEDLEKAKAFFDKAGLPAT
                     WVEVAHQGRTLHVVDANGAPIEFCATMEVKPRLVVAFEHHHGAVPQRIDHFQLLVPDV
                     QRACEFWMSLGFRLSEYISPDGSEELLFVFLQRKGNPHDIVFAPGAGPRLHHAAFSIP
                     ESYHFFYVCDLAAEMGFAANIEFGPGRHGPGHALFVYLRDPDGHRIELFNTHYQVMDI
                     ENEPVRWDASHAMKRRWQLPARRQWFVEASRFAGIEPREPARKGDPMSLEKFIAAGLR
                     "
BASE COUNT          451 a          827 c          796 g          472 t
ORIGIN      
        1 gcctgcgaga ccgccgttct caccctcggc ggcatgggtt attcgaagga attccacgtc
       61 gagcgctatc tgcgggaggt catgatcccg cgaattgccc cgatcacgcc acatatgatc
      121 ctaaatttca tcgcagagaa gattctcggc ctgccgaagt cctactagga gggctgccgg
      181 cgggatgagc ccgggcgcga agacaagctc ggtctgctcc ctcttccact tgcgggggga
      241 ggggtggggt gggggtaatt ggaaatgact ttgtcccggc ggatggctcc ggtcatcgcc
      301 ctggcgctgc tgctggggca gagcgcatgg cattgcgcgt gggccgaagg ccccggtggt
      361 ccggcgaaga aattagcacg ttcggcctgc gaccatgcgg cctttcgcgt cgtcgtggac
      421 gtcgggcata cggccgagtc ccccggcgcc cccagcgcgc gcggcttctg ggagtatgac
      481 ttcaaccttc ggctcgcgac gctgatcaag caaaaattgc tcgctgccgg ctttgccaag
      541 accgtcttgc tggtcaccga tgggccggcc aggaaaagcc tgttcgcgcg cgcggcgcgc
      601 gccaatcagt tgtctgccga cctcttcctc tcgatccatc acgactcggt gccggattcg
      661 tttctggaaa gatgggagta cgaaggccac gagcacggct tcagcgaccg gttcaaaggc
      721 cactccatct tcttctccaa cgagaatgtc gatcctgatg ccagccttat gtttggccag
      781 ctgctcggcg agcagttgca ggcccgcggc ttgcaataca ccgcccacta catcgaaaaa
      841 ttcatgggcc accggcaaag gctgctggtg gacgccaagg tgggtgtgta cagatacgat
      901 cagctgatcg tgctgaggac gacgcagatg cctgcggtct tgctcgaggc cggctcgatc
      961 atcaatcgcg ccgaggaatt ggtaatgaac tcgccggagc gccaatcgct catcggcgcg
     1021 gccgtgaccg acgcggtcga aggcttttgc gccatacggc ggccgcgaaa tcccgatcgc
     1081 gttgccggcc cgccgcgcgc ggccgccaaa ctcgctcccg acgtggcgcc gcagccggca
     1141 agccctgccg gagcccaata agcacttaat acgccggccg aataccggcc aatagcagca
     1201 ctgcccggcg atcccgatca ccaccgtgtc gcgcaccggt gtccttcctc cgcgctatgg
     1261 aagctgaaca tatggcagcg gccagaccga gctgcgccca cgccgattcc atttcacggg
     1321 aacgtgctct agaacggtct ccgattgaac atcctcgatt ggagcgcgcc aatgagcatt
     1381 ccaaagcccg tctacaatcc gcccttcaac gtcacgcgcg cgagccattc ggtgctcacc
     1441 gtgaaggacc tggcggtgag ccgaaacttt tacgtggatc tcctcggctt catcgtcagt
     1501 gatgaagaca gcgatgccct ctatttgcgc ggcgtcgccg aggcgtgtca tcacagcctc
     1561 gttctcaagc gcgcgcgcgg cgagccgcaa gccgagcgcg tcggcatgcg tgtcttcagc
     1621 gacgaggatc tggagaaggc caaggcgttt ttcgacaaag ccggcctgcc ggcgacatgg
     1681 gtcgaggttg cgcaccaggg ccgcacgctg catgtcgtgg acgcaaacgg cgctccgatc
     1741 gagttctgcg cgaccatgga ggtcaagcca cgcctcgtgg tcgcgttcga gcaccaccac
     1801 ggcgcggtgc cgcagcggat cgatcatttc cagctcttgg ttcccgacgt gcagcgcgca
     1861 tgcgaattct ggatgagctt gggctttagg ctctcggaat acatctcgcc ggacggaagc
     1921 gaggagctgc tgttcgtgtt cctgcagcgc aagggcaacc cccacgatat cgtgtttgcc
     1981 cccggggcag ggccgcggct gcatcacgcc gcgttctcga tcccggaatc ctatcacttc
     2041 ttctatgtct gcgatctcgc cgccgagatg ggctttgccg ccaatatcga gttcgggccg
     2101 ggtcgccatg gccccggcca cgcgctcttc gtctacctgc gcgatccgga cggccaccgc
     2161 atcgagctct tcaataccca ttaccaggtg atggacatcg agaacgagcc ggtgcgctgg
     2221 gacgcgagcc atgccatgaa gcgccgctgg caacttccgg cgcggcggca atggttcgtc
     2281 gaggcgagcc gctttgcagg catcgagccg cgcgagcccg cgcgcaaggg cgacccgatg
     2341 agcttggaaa aattcatcgc cgccggcttg cggtgagcac atcactgccc cagcaacccg
     2401 gcgctcgcgg ccgcggcgtc ccagatcctg gcgtcttcct cgatcatggc ggcgaacgcg
     2461 gaaggcgtgt tggcgatggt ctcgagcccg ttgtcgccga gaacgcgctg cgcctccgcg
     2521 gagttgagga tgcgcacgac ctcgtc
//
LOCUS       DATLJT010001508         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4275560,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001508 DATLJT010000000
VERSION     DATLJT010001508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4275560"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VM910_34490"
     CDS             complement(<1..1035)
                     /locus_tag="VM910_34490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HVI67654.1"
                     /translation="MTRRTRSALTRRQLLIRSASLVTLAGTSSLAKPYLSRAADRPQI
                     AGGIQSGDVDGDRAIVWARADRAARMRLECSTSDSFKTILCAAFGDAVPDRDFTAKLP
                     VENLPGGQEIFYRVQFDDIASNLAGETRIGRFRTPSRTRKSIRFLWSGDTAGQGWGID
                     PARGGYRTYRTMLENRPDFFIHSGDHIYADCTIPAEQGLPNGEIWRNITTEAKSAVAH
                     TLQQFRGNYQYNMLDENFRAFHAQVAMLAQWDDHEVTDDWSPRGSVDADGYDDNGTSR
                     LVSRARRAFFEYMPIRETRAQAGRIYRRISYGPLLDIFLIDMRSYRDSTWNKGDDRDS
                     FILGATQLAWL"
     gene            complement(1162..1590)
                     /locus_tag="VM910_34495"
     CDS             complement(1162..1590)
                     /locus_tag="VM910_34495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67655.1"
                     /translation="MALKHLFAASLGAALICASASMAADYSSDEFFGLDLSKAVMSPK
                     PLGPPATFEPVPVEAKSDRASEPASARDELKTEPRKVAVETAKARHRRHVAAAVSQKS
                     KASGRTHLAHRHRNPLDAQAMDTRIQTWPCKSGGICNWRQ"
     gene            complement(1753..3204)
                     /locus_tag="VM910_34500"
     CDS             complement(1753..3204)
                     /locus_tag="VM910_34500"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI67656.1"
                     /translation="MYRDIRNSSKIGAVLRATVLCVGMVSLVSASGSAAAADALTPSQ
                     ALAHDIYKELLEIDTTTATGDTKAAADAMAARLKAAGFTDADVQVFQPAPRKGNLVAR
                     LHGSGKRKPILLVAHLDVVPALREDWSVDPFKLTEQDGYFYARGSLDDKYMAASFVTN
                     LIRYRQEGFKPDRDIIVALETDEEIFDRDGLGIQWLLKNHRDLIDAEFALNEGGGVAV
                     KDGKPIRNSVQTTEKVAVTFQLDVRDRGGHSSVPRKDNAIYRLAEGLVKLSKFSFPMR
                     LNETTRAYFERTAQFESEQTAADIRAVLSDKPDPSALSFVRLAANPVYNAQLRTTCVA
                     TMLQGGHAENALPQLASAKVNCRIMPGEPVEEVKATLGRVLDDDQITVTQLAGAVVSA
                     PSALHEEIVGTIEKLTQEFFPGAVVLPIMSSGATDGSYLRNAGIPTYGHSGMAVDINE
                     SRVHGRDERAPQKSFYQGNEYLYRLVKMLSGGA"
     gene            complement(3306..>3732)
                     /locus_tag="VM910_34505"
     CDS             complement(3306..>3732)
                     /locus_tag="VM910_34505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI67657.1"
                     /translation="EVIRHPIPNSTFPIAQAVEVRGNVTTYYVSGQVPPVVNKDADPS
                     TSAAYGDTKTQTVGVLTRIKGILEGFGLSMADVVKMQVFLVHGAAAPMDFKAFMEGYT
                     QFFGSSQPDLPARSVVGVASLANPGFLVEIEVIAVKDAK"
BASE COUNT          665 a         1168 c         1185 g          714 t
ORIGIN      
        1 cagccaggca agctgcgtcg cgccgagaat gaagctgtcg cggtcgtcgc ccttgttcca
       61 ggtcgaatcg cgatagctgc gcatgtcgat caggaagatg tcgagcagcg ggccgtagga
      121 aatccggcgg tagatgcggc cggcctgcgc tcgtgtctcg cggatcggca tatactcgaa
      181 aaaggcgcgc cgggcgcgcg aaacgagccg cgacgtgccg ttatcgtcat agccgtccgc
      241 atcgacactg ccgcgaggcg accagtcgtc cgtcacctcg tggtcgtccc actgcgcgag
      301 catcgccacc tgcgcatgaa aagcgcgaaa attctcgtcc agcatgttgt attgataatt
      361 gccgcggaat tgctgaagcg tatgggcgac cgcggatttt gcctcggtcg tgatgttgcg
      421 ccagatttca ccgttcggca ggccctgctc ggccggaatc gtgcagtcgg cgtagatgtg
      481 gtcgccggag tgaatgaaaa aatcgggccg gttctccagc atcgtcctgt aggtgcgata
      541 tccccctcgc gccggatcga ttccccagcc ctggcccgcg gtatcgcccg accagagaaa
      601 gcgaattgac ttacgcgtcc gcgagggggt gcggaaacgg ccgatccgcg tctcgcccgc
      661 tagattggat gcgatatcgt cgaactgcac gcgataaaag atctcctgtc cgccgggcag
      721 gttttccacg ggcagctttg ccgtgaaatc gcggtccggc acggcatcgc cgaatgcggc
      781 gcacaggatg gtcttgaaac tatcgctggt cgagcattcg aggcgcatgc gcgccgcccg
      841 gtcggcgcgg gcccacacga tcgcacgatc gccgtcgaca tcgcccgact ggatgccgcc
      901 cgcgatctgc ggccggtccg cggcgcggct gagatagggt tttgcaagag agctcgtgcc
      961 cgcgagcgta accagggagg cggagcggat cagaagctga cgtctggtca gggccgaccg
     1021 ggtgcgtcgt gtcattcgtc gggcaacctt catcgaatcg cgatgaagcg tgcccgtcga
     1081 gtttgactct gggccgaagg tttcttgact cgtgctttaa ggttttacga cggagaagtg
     1141 actccggccg aaccgaaccg ctcactgcct ccagttgcag atgccgccgg acttgcaggg
     1201 ccaggtctgg atgcgcgtat ccatcgcctg cgcatccagc ggattgcggt gtcggtgtgc
     1261 gagatgggtg cggcccgacg ctttcgattt ttggctcacg gccgcggcaa catggcgccg
     1321 atgcctcgcc ttggccgtct cgaccgcgac ctttcggggt tccgtcttca gctcatcgcg
     1381 cgccgaggcc ggctcgctgg cgcgatcgct cttcgcttcc acgggaaccg gctcgaatgt
     1441 cgcgggcggg ccgagcggct tcggcgacat cactgccttc gacaaatcga gtccgaagaa
     1501 ctcgtccgag ctgtagtcgg ccgccatcga cgcgctggcg caaatcagcg ctgcgccgag
     1561 cgaggcagca aaaagatgtt tcaaggccat tctggcctcc caaaagtccc aagaaaaaac
     1621 cacttccctc acgatctatt tagcagttcg tcggttcggt gccagcggca aatgctttaa
     1681 tagttaccgc tcgagacctc gccggcgaat tcgcccgaaa atcgttccag agcgagccgc
     1741 gcgcgggcgg ccttaagccc cacccgatag catcttgacc agccggtaga gatattcgtt
     1801 gccctgatag aacgattttt gcggagcccg ctcatcccgg ccgtgaactc ggctctcgtt
     1861 gatgtcgacg gccatgccgg aatggccgta ggtgggaatc ccggcgttgc gcagatagct
     1921 cccgtcggtc gcgccggaac tcatgatggg caacacgacg gcgcccggaa agaattcctg
     1981 cgtcagcttc tcgatcgtgc ccacgatctc ctcatgcagg gcagacggcg cgctgaccac
     2041 cgccccggcg agctgggtga cggtgatctg atcgtcatcg agcacgcgcc ccagggtcgc
     2101 cttgacctcc tcgaccggtt cgcccggcat gatgcggcaa ttgaccttgg cgctcgcaag
     2161 ctgcggcagc gcgttctccg catgtccgcc ctgcaacatg gtcgcgacgc aggtggtgcg
     2221 tagctgcgcg ttgtagacgg gattggcggc aagccggaca aaggacagcg cggatgggtc
     2281 cggcttgtcc gacagcaccg cacggatgtc ggcggcggtc tgctcactct cgaattgcgc
     2341 agtgcgctcg aaataggcgc gcgtcgtctc gttgagccgc atcgggaaag agaatttcga
     2401 cagtttcacg aggccttcgg cgaggcgata gatcgcgttg tccttgcgcg gcaccgagga
     2461 gtggccgccg cggtcgcgaa cgtcgagttg aaaggtcacc gcgacctttt cggtggtctg
     2521 cacgctgttt cgtatcggct tgccgtcctt caccgcgacg cccccgccct cgttgagggc
     2581 gaactcggcg tcgatcaggt cgcgatggtt cttcaaaagc cactggatgc cgaggccgtc
     2641 gcggtcaaaa atctcctcat ccgtctccag cgcaacgatg atgtcgcggt cgggcttgaa
     2701 gccttcctgc ctgtagcgga tcagattggt gacgaaggat gccgccatgt atttgtcgtc
     2761 gaggctgccg cgcgcataga aataaccgtc ctgctcggtc agcttgaagg gatcgaccga
     2821 ccagtcctcg cgcagggccg gaaccacgtc gagatgcgca accaaaagga tcggcttgcg
     2881 cttgcccgag ccgtgcagcc gcgccaccag attgcccttg cgcggcgccg gctggaacac
     2941 ctggacgtcg gcatccgtga atcccgccgc cttgaggcgc gcggccatcg cgtccgccgc
     3001 agccttggtg tcgcccgtcg cggtcgtggt gtcgatttcg agcagctcct tgtagatgtc
     3061 gtgcgcgagc gcctggctcg gcgtgagcgc gtcagcagcg gcggcgctgc cgctcgcgct
     3121 caccagcgag accatgccga cgcaaagcac ggtcgcgcgc agcaccgccc cgatcttgct
     3181 cgaatttctg atgtcccgat acatcccgca ctccagggaa ttctgacccg tttcgattga
     3241 tcaagacagg cgtcgggcct tccggcaagc ctcggagccc gtcgatggaa gcgcatctga
     3301 cagcgctatt tcgcatcctt cacggcgatc acctcgatct cgaccagaaa gccgggattg
     3361 gcaagcgagg cgacgccgac gacggagcgt gccggcagat cgggctggct gctgccgaaa
     3421 aactgggtgt agccttccat gaacgccttg aagtccatcg gcgcggcggc gccatgaacc
     3481 aggaacacct gcatcttgac gacgtcggcc atggagagtc caaagccctc caggatgccc
     3541 ttgatccggg tcagcacgcc gacggtctgg gttttggtgt cgccatacgc cgcggaggtt
     3601 gagggatcgg catccttgtt gacgacgggt ggcacctgac cggaaacata ataggtcgtg
     3661 acattgcccc tgacctcgac agcctgcgcg atcggaaatg tcgagttcgg aattggatgt
     3721 ctgattactt cg
//
LOCUS       DATLJT010001509         8483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2669503,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001509 DATLJT010000000
VERSION     DATLJT010001509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669503"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VM910_34510"
     CDS             <1..153
                     /locus_tag="VM910_34510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HVI67658.1"
                     /translation="AYRVMMTAHRRGVCVVAVYTRDVAETKATRGTEAGRRKGYPLQF
                     TTEPEE"
     gene            320..1588
                     /locus_tag="VM910_34515"
     CDS             320..1588
                     /locus_tag="VM910_34515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67659.1"
                     /translation="MARTIRDAALETRAARSRLKPNGQPYYRGLEPGLHLGYRKPRAG
                     AGKWLARLNVEGKYNYHPLGTADDFSDPDGAIILSYKQAQDAARKVQVGRGIGTVGDA
                     VEAYIRSLEAEGRARTSLAVLRCSANAHILPALGHVELAKLTSEQLQRWRDDLARKSA
                     HVRTRSGEPQRHRARSDDTDVSRARRASANRVRAMLFRALNLAYEHGHVASDQAWRRV
                     KPFKGVTKARLRYLTMAEAKRLINAACPEFRPLVQAALLTGARYGSLGQLTASDLSLD
                     AGTLRLRTRKGDGSERVFHVHLTDEAWEFFQAACAGRRGNDLIFTRTDGRSWGKSQQQ
                     ALMREASERAGIAPAVNFHATRHTFASHAVMNGAPLLVVAQALGHMGTTMVEKHYGHL
                     APSYAADAIRKAAPRYGIRPGNVRPLHGKE"
     gene            1700..2548
                     /locus_tag="VM910_34520"
     CDS             1700..2548
                     /locus_tag="VM910_34520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67660.1"
                     /translation="MSENSQRRHKPQYSSAFNARSLPEQESVSGAFVAVEGADAATFA
                     GQVDELAEGPTLPLDSSNWLTTGYVYQSLCKLPRYVGYSGLALADLYAKVRSGDVPAM
                     RQCFLYDCNRKLPGPARERLKRSHWRAHRFLGQPDGRLEVIEGPFHSAVRPVIYGPVR
                     GYAYFVWKPAVAKHWPGVFASTPLPPALTNPVDGKQAPGGKQAPQRRRGPVTTHDWCS
                     IWGEIACRCIDPKSQRLRIPKSERKLARDMLTWCQTTYGQEPAESEMREAVKGVCAAL
                     RRGGSK"
     gene            2631..3089
                     /locus_tag="VM910_34525"
     CDS             2631..3089
                     /locus_tag="VM910_34525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67661.1"
                     /translation="MARPEITGRKVADAEGWVVAHTIRESDLVLKKGTLIGKAEIAAL
                     KAAGIAEIVVVRIKPKTQIKPKRPARTKKRQRARFRNLQPGAYTIPSFCVAHDLSESF
                     YHKLRSLGLGPREMRVERRVLISHEAASDWRKEREEATPRALPPPSPEAA"
     gene            3181..4551
                     /locus_tag="VM910_34530"
     CDS             3181..4551
                     /locus_tag="VM910_34530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67662.1"
                     /translation="MLLLAERAQKVKFDSATASATVSWRDNLLIHPAVELFPPMSSDE
                     LRALGEDIVKNGFTSPIVLWRARPNERAILLDGRSRLDATEMATGRAVEVDGSSVMAG
                     EFLATNKVIVLDGRSVDPWAYAISANIRRRHLTIEDTDRLTVELLKADPTKSSRQVAK
                     IVGASRPHVAKVRRQAEQAGHVETVTTTVDTKGRGQPAKRPRNQTRAVRAVAVSEMGA
                     PRFAADHDGGDSADHGDARGGGRGDKHAEQPAISMQARIDELTHEVRVRDIRIHALEG
                     EIADLKTAAAEACQQSADLVTAWNNATDAELAAGLTAIGLSAVLRVMPGWKTETVRRI
                     TDWPQGRPAGHNPLTAALRSALSHLAAATAPQADVQAEELAALNALRGITRLNPNYHT
                     LTITVQKERRPSRRQCRAAYAESSDGPPHPLRDKEKAQRFRRWASEANMEPSRGCISD
                     CDQAHY"
     gene            4514..5011
                     /locus_tag="VM910_34535"
     CDS             4514..5011
                     /locus_tag="VM910_34535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67663.1"
                     /translation="MGAFQTAIKPTTNGSNSSAVSTVMPAGATALPANSKALVPVKHQ
                     RHYQARGRRFRHGVCAAALRAVTAARMLLRGEFSSLIEAADGCGSCPQYVVYGRTILE
                     SGNPALLEDVLAGNVPLREAAEREWNVVQLQLAFRRASAAERIRFGRREGVGTLWDEC
                     IAPAL"
     gene            5254..5826
                     /locus_tag="VM910_34540"
     CDS             5254..5826
                     /locus_tag="VM910_34540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI67664.1"
                     /translation="MFALDATMSREWAWDASVKLQADMFAEAAKLGGLEMQLVWYRGL
                     DECKASHWTIDARALATAMSGIKCVAGLTQIGRLLTHARREHERQKVNAVIFVGDAVE
                     EKPHELYDLAGGLGVPVFLFQEGNDATVEKIFAQLARLTNGAHLKFAPGSLHELGELL
                     RAVAVFSVGGTAALADLRTNAAHKLLGQLK"
     gene            5826..6440
                     /locus_tag="VM910_34545"
     CDS             5826..6440
                     /locus_tag="VM910_34545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67665.1"
                     /translation="MGKHGDEYPRVPRDDYPTPAWVIDALAEHVELRGLTILEMACGK
                     EGRMSEALKAAGCARVYSSDIVNGYAGQDEVLDFLSGRLPGLPHCDAAVTNPPFGPRG
                     KTAEAFITRSLQLLTSGYTDFVALLLPHDFDSAKTRTHLFERCPQFIGKITLRRRVKW
                     FEHSDLVRRKRHPKENTDWYLFKRGSQRDRRPPFILYAPGSHKG"
     gene            6444..6749
                     /locus_tag="VM910_34550"
     CDS             6444..6749
                     /locus_tag="VM910_34550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67666.1"
                     /translation="MARLTLTPNLTSGHSDELAQQIRATYEGQAYFANTGPFGATCHE
                     CIFWGYYQQRRDRAGNSTKTTFRKGCAKYHALTGKHGAVVPATAPACRYFERKEGGR"
     gene            6786..6968
                     /locus_tag="VM910_34555"
     CDS             6786..6968
                     /locus_tag="VM910_34555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67667.1"
                     /translation="MTDTSPVIDRKDLSDDLESLSDQIASLERAIKSLRCELDEVEFA
                     LAGVNTAFEDVVKNIK"
     gene            7131..7748
                     /locus_tag="VM910_34560"
     CDS             7131..7748
                     /locus_tag="VM910_34560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67668.1"
                     /translation="MPINLNDAGPQQGNHDLLPAGPYHFGAIVIPGGAGLDGFLRREK
                     KNPRVLMMEVECKVTDGDYANRRVWDYIPVEIDQSTDGNLAPIPADQLSKIQSRVRRG
                     RAKLRALLDSAFELQPNDDSDAAQKKRELDSYGAVHGLKFYAQVEEQAGSAGYGPSNR
                     IDFIITPDLPDYPRQPITAVTSTVAPPKRSLPFGDDEIPFAPNFD"
     gene            7766..7939
                     /locus_tag="VM910_34565"
     CDS             7766..7939
                     /locus_tag="VM910_34565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67669.1"
                     /translation="MGEHEPCIGATSEWFTPPEYFQALDLIFDLDPCSPGPGHWVPAE
                     KVYTIEDDGLRQP"
     gene            7936..8097
                     /locus_tag="VM910_34570"
     CDS             7936..8097
                     /locus_tag="VM910_34570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67670.1"
                     /translation="MIEHIKALEKKYPGLDRISVSLSVGVPEKVALEQLDRFAKEVMP
                     AFKEANRRC"
     gene            complement(8250..>8483)
                     /locus_tag="VM910_34575"
     CDS             complement(8250..>8483)
                     /locus_tag="VM910_34575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67671.1"
                     /translation="RKLLDRNGDAQSKAPGFVSRQTLIALADPAKITTLVVWTTNDIY
                     EAWGVSPQRTMAMSEADRLWAKPPESERFQLVG"
BASE COUNT         1743 a         2620 c         2579 g         1541 t
ORIGIN      
        1 gcctatcgcg tgatgatgac cgcccatcgg cgcggcgtat gcgtggtcgc cgtctatacc
       61 agggacgtcg ctgagaccaa ggccacgcgc gggaccgagg ccggccgccg caagggctat
      121 ccgttgcaat ttaccaccga gccggaggaa taggtgggca aaatccgctc caccgggctc
      181 catggggctc ttgcaaaacg ccgatgaaat gggcatttcc tccattggag tagatagtgc
      241 tcgacagagc tccacccaca cattcttcac atattcttca catgcgtacg accgtacgag
      301 ctccacaggg taaattcaca tggcccgcac aattcgtgac gccgctctcg aaacccgcgc
      361 cgcccgcagc cggctgaagc cgaacggcca gccctattac cgcggcctcg agcccggact
      421 tcatctcggt taccgcaagc cgcgcgccgg cgccggaaag tggctcgccc gcctcaacgt
      481 cgaaggcaaa tacaactacc atccgctcgg cacggccgat gacttcagtg accccgacgg
      541 cgcgatcatc ctctcctaca agcaggcgca ggacgccgcc cgtaaggtcc aggtgggccg
      601 cgggatcggc acggtgggtg acgcggtgga ggcgtacatc cgttcactgg aggccgaggg
      661 tcgcgcacgg acatcgctcg cggttctgcg ttgcagtgcc aatgcccaca tcctgccagc
      721 gctcggacac gtcgagcttg ccaagctcac ctccgagcag ctgcagcgct ggcgcgatga
      781 tcttgcccgc aagtcggcgc acgtgcgtac ccggagcggt gaaccgcagc gccatcgtgc
      841 tcgcagtgat gacactgacg tcagccgcgc ccgtcgggcc agtgcgaacc gggtccgcgc
      901 catgcttttc cgcgcgctga atcttgctta cgaacacggc cacgtggcga gcgatcaggc
      961 ttggcgtcgg gtcaagccgt ttaagggtgt caccaaggcg aggctgcgct acctgacgat
     1021 ggcggaagcc aagcgcctca tcaatgccgc ctgtccggag tttcgcccgc tggtgcaggc
     1081 ggcgctgttg accggcgcgc gctatggctc gcttggccag ctcacggcgt cagatctcag
     1141 ccttgatgct ggtacgctgc gcctgcgcac caggaagggc gacgggagtg agcgcgtctt
     1201 ccacgtgcac ttgaccgacg aggcgtggga gttctttcag gcagcctgtg ctgggcgccg
     1261 cggtaacgat ctgattttca cccgcaccga cggccggtca tgggggaagt cgcagcagca
     1321 ggctctaatg cgggaagcct cagagcgcgc cggtattgcg ccggcggtca atttccacgc
     1381 tactcggcac accttcgcca gccacgccgt gatgaacggt gccccgttgc tcgtggtcgc
     1441 gcaggcgctc gggcatatgg gtacgacaat ggtggaaaag cattacggcc atcttgcccc
     1501 cagctatgcc gccgacgcga tccgcaaggc ggctccgcgc tacggcatcc ggcccgggaa
     1561 cgttcgaccg ctgcacggca aggagtgaag gttgcagccg aggttgtgat gagctgagaa
     1621 tgtgagagcc gggtaccgag tggttcgttc gtacccggcc ctcgaaagca tgttcccacg
     1681 ccaagcgggt ccacgctgca tgagtgaaaa tagtcaacgc cgacacaagc cgcaatattc
     1741 ctccgccttt aatgcccgct cgctgccaga gcaagaaagc gtttccggcg cgttcgttgc
     1801 cgtcgaaggt gccgacgccg ctacatttgc tggacaggtc gacgagcttg ctgagggccc
     1861 gacactgccg ctcgacagct ctaactggct aacgactggc tacgtttatc aatcgctgtg
     1921 caaactaccc cgttatgtcg gctattctgg tttggcttta gcagatctgt acgcgaaggt
     1981 gcgcagcggt gacgtgcccg cgatgcgtca atgctttcta tatgattgca atcggaagct
     2041 gcccggtccg gcgcgtgaac ggcttaaacg ctcgcactgg cgcgcgcaca gattcttggg
     2101 ccagccggac ggtcgcttgg aagtcatcga aggtcccttc cacagcgctg tgcgcccggt
     2161 catctacgga ccggtcaggg gctatgctta tttcgtctgg aagcccgcgg tcgcaaagca
     2221 ttggcccggt gtgtttgcat cgacgccatt acctccggcg ctgacgaatc cggttgacgg
     2281 taaacaggct cctgggggta agcaggctcc ccaacgtcga cgtggaccgg tcacgacgca
     2341 cgattggtgc agtatctggg gcgaaatcgc ttgccgctgt atcgacccaa aaagtcaacg
     2401 gttgcgaatt cctaaaagtg agcggaagct tgccagagac atgcttactt ggtgccagac
     2461 cacatacgga caagagcctg ccgaaagcga aatgcgtgaa gccgtgaagg gcgtctgcgc
     2521 ggcgctgcga cgcggagggt ccaagtaatt ccggccaata ccggccaatg ccggccacct
     2581 gaaaacttgc gctagcgtat cgtgctccat cgtctctgat ggaggccgga atggctcggc
     2641 cagagatcac cggtcgcaag gtggccgacg ctgaaggctg ggtcgtagcc catacgatcc
     2701 gcgagagcga cctcgtgttg aagaagggca ctctgatcgg taaggccgag atcgccgcgc
     2761 tcaaggctgc aggcatcgcc gagatcgtgg tggtgcggat caagcccaag acgcagatca
     2821 aacccaaaag acccgcccgc actaagaaga gacaacgcgc ccgcttccgt aacctgcagc
     2881 ccggtgccta tacgatcccg agtttctgcg tcgctcacga tttgagcgag tccttctacc
     2941 acaagctccg gtctctcgga ctcggcccgc gcgagatgcg ggtcgagcga agggtgctga
     3001 tctcacacga ggccgccagc gattggcgca aagaaagaga agaggccacg ccgcgcgcat
     3061 tgccaccacc ctctccggag gccgcatgag aacttggtga gtggtgggcg ccgctaggaa
     3121 cggcgcgcca ccctagaagg aagattgtcg gaatacccag tccaaaggag gactaaagcg
     3181 atgcttctac tcgcggaaag agcccaaaaa gtcaagtttg atagcgccac cgcgtccgcc
     3241 accgtcagct ggcgcgataa tctcctaatc catccggccg tcgagctttt cccaccgatg
     3301 tcgtccgacg agctgcgcgc gcttggggag gacattgtga agaacggctt caccagcccg
     3361 atcgtcctct ggcgcgctcg accaaacgag cgagcaatcc tgctcgacgg caggagtcgg
     3421 ctcgacgcaa ctgagatggc tacgggtaga gcggtcgagg tggatggttc aagcgttatg
     3481 gcgggcgaat tcctcgccac caacaaggtg atcgttctcg acggccggtc ggtcgatccc
     3541 tgggcttacg cgatctctgc caacattcgc cgtcgacatc tcacgatcga ggacacggac
     3601 cgacttaccg tcgagttgct caaagccgat ccgacaaagt cgagccggca agtagcaaag
     3661 atagtgggtg ccagccgtcc gcatgtcgcc aaagtgcgca ggcaagcgga acaggccggt
     3721 cacgtggaaa ccgttaccac gactgtcgac accaaaggtc gcgggcaacc ggcgaagcgc
     3781 ccgagaaacc agactcgcgc cgtgcgtgcg gtcgctgtca gcgaaatggg tgcgccaagg
     3841 ttcgccgctg atcatgatgg gggcgatagt gctgatcacg gcgacgctcg cggtggcggt
     3901 cgcggcgata aacatgctga acaacccgcc atctcaatgc aggcgcggat tgatgagctc
     3961 acgcacgagg tccgtgtccg tgacatccga attcatgccc tagagggtga gatcgcggat
     4021 ctaaagacag ccgcagccga ggcgtgccag caatcagccg atctggtcac agcctggaat
     4081 aatgcgacgg acgcggaact ggctgcggga ttgaccgcca ttggcctcag cgccgttctc
     4141 cgcgtcatgc cgggctggaa aaccgaaacg gttcggcgga tcaccgactg gccgcaaggg
     4201 cgtcccgccg gtcacaatcc gctaacggcc gccttgcgaa gcgcactttc tcaccttgcc
     4261 gcggcaactg ctccgcaagc cgacgtccag gcggaagaac tcgcagcctt gaacgccttg
     4321 cgcggcatca cgcggctcaa tccgaactat cacaccctaa caatcaccgt tcagaaggaa
     4381 cgccgccctt cgcggcgcca gtgtcgtgca gcttacgctg agtcatccga cggtccgcct
     4441 cacccgcttc gggacaaaga aaaagcccag cgtttccggc gctgggccag tgaggcaaac
     4501 atggagccct cacgtgggtg catttcagac tgcgatcaag cccactacta atggcagcaa
     4561 ctcgtcagcc gtctccaccg tgatgcctgc gggcgccacc gcgttgccgg caaactcgaa
     4621 ggcgctcgtg ccggtcaagc atcagcgtca ttaccaggcc cggggacggc gatttcggca
     4681 tggggtatgc gcggccgccc ttcgcgctgt gactgcggcc aggatgctcc tgcgtggcga
     4741 attctcgtcc ttgattgagg ccgccgacgg ctgcggatcc tgtccgcaat acgtcgtgta
     4801 cgggcgcacc atcctggaaa gcgggaaccc cgcgctgctg gaggacgtgc tggcaggcaa
     4861 cgtccctttg cgtgaggccg cggagcgcga gtggaacgtc gtacagctgc aattggcgtt
     4921 tcggcgggct tccgctgccg agcgcattcg cttcggtcgc cgagagggtg tgggtactct
     4981 ttgggatgag tgcatcgcac cggccctcta acgcgtgtcg aggagattga ggttcaagga
     5041 acccaaggac gctggctacc tccgagccgg ctagtggcgg ggtcgggcac cgaaagacag
     5101 cctgcggtgc gcccttggga gagttcactc agccgtacaa ggttgtcggc cgccacactc
     5161 attcagaggt tcctcatgac gcagactctt ccatcgtcca gcgctcgcgt gggcgaattt
     5221 ttgaagaaaa tgcaaacggc gcgagggcgc ctgatgttcg cgctcgacgc cacaatgagc
     5281 cgagagtggg catgggacgc ctcggtgaaa ttgcaggcgg atatgttcgc ggaggcagcc
     5341 aagctcggcg ggctcgaaat gcagctcgtc tggtaccgcg gattagacga atgcaaggcg
     5401 agccattgga ccatcgatgc acgcgcgctc gccaccgcga tgagcggaat caagtgcgtg
     5461 gccggtctta cccagattgg cagattactg acgcacgctc gcagagagca tgagcggcag
     5521 aaagtgaacg ctgtcatttt tgttggcgat gcggtggagg aaaaaccaca cgagctttac
     5581 gaccttgcgg gcggactcgg cgtgccagtc tttttgtttc aggaaggcaa cgatgcaacc
     5641 gtcgagaaga tcttcgcgca gctcgccagg ttaaccaatg gcgcgcatct caagttcgcg
     5701 cccggttcgt tgcacgagct cggcgaattg ttgcgcgcag tcgccgtgtt ttccgtcggc
     5761 ggcacggcgg cgttggctga tctacgcacg aatgctgcac acaagttgct tggacagctg
     5821 aaataatggg taagcacggc gacgaatatc cgcgcgtccc gcgcgatgat tatcccaccc
     5881 cagcctgggt gatcgatgcg cttgccgagc acgtcgagct gcgcgggctg actatcttgg
     5941 agatggcgtg tggcaaggaa ggacgcatgt cggaggccct caaggccgcc ggctgcgcgc
     6001 gtgtctattc cagcgatatc gtcaacggct acgccggtca ggacgaggtg ctcgacttcc
     6061 tctcagggcg gcttcccggc ctgccgcatt gcgatgcagc ggtaaccaat ccgccgttcg
     6121 gcccgcgcgg aaagacggcg gaagcgttca ttacgagaag tctgcagctg ctcaccagcg
     6181 gctacaccga ttttgtcgcg ctcctacttc cacacgattt tgatagcgcc aaaacgcgca
     6241 cacatctgtt tgagcggtgc ccgcaattca tcggcaagat cacattgcgg cgacgcgtga
     6301 aatggttcga gcattcggac cttgttcggc gcaagcgcca tcctaaagaa aacacggatt
     6361 ggtatctgtt caaacgcgga tcacaacgcg atcggcgacc acctttcatc ttgtacgcac
     6421 caggtagtca caaaggctga accatggcgc gcctcaccct cacaccgaat ctgacctcgg
     6481 gccattccga cgagctcgct cagcagatcc gcgccaccta tgagggccag gcgtatttcg
     6541 ccaacaccgg accgttcggc gcgacctgcc acgaatgcat tttctggggc tactaccaac
     6601 agcgtcgcga tagggccggc aactccacca aaaccacgtt tcgaaaaggc tgcgccaagt
     6661 accatgcgct caccggcaaa catggtgcag tcgtgccagc aaccgcgcca gcgtgccgat
     6721 atttcgaacg caaggaaggc ggcagatgac caaccgcttt accctcgaaa cacaaggaga
     6781 tgactatgac tgacacgtcc cccgtcatcg atcgcaagga tctcagcgat gacctcgaat
     6841 cgcttagcga ccaaatcgct tcgctcgagc gggcgatcaa gtcgctacgt tgcgagctcg
     6901 atgaagtcga gtttgcactc gctggcgtga ataccgcgtt cgaggacgtg gtgaagaaca
     6961 tcaaataacc ctgccaatgg cgcgcccaac gttttgtact gcgcgtgcgt ggcgaaccgg
     7021 gcaccgatgt aattcgaagt ttaagaggtt ggctcaaaca aggtttgcgt gagtttggtt
     7081 tgcgctgcct cgacgtccac caggaaagcg aaactcagga ggaggtatct atgccgatca
     7141 acttaaacga cgcgggcccg caacagggca atcatgatct gctgccagcg gggccctacc
     7201 acttcggggc tatcgttatc cctggcgggg ctggcctcga tggcttcttg cggcgagaga
     7261 agaagaaccc gcgtgttctg atgatggagg tggagtgcaa ggtcaccgac ggcgactacg
     7321 caaacagaag agtatgggat tacatcccag tcgaaatcga ccaaagcacg gatggcaatt
     7381 tagcgccgat ccccgccgat caattaagca aaatccagag ccgagtgcgg agggggcgcg
     7441 caaaattacg tgcccttctc gattccgcct ttgagctcca acccaatgac gattccgatg
     7501 ccgcgcaaaa gaagcgcgag ctcgacagct acggcgccgt ccacggcctg aaattctacg
     7561 cccaggtcga agagcaagct ggcagcgccg gttacggacc atccaaccgc atcgacttca
     7621 tcatcactcc cgatctgccg gattacccac ggcaaccgat aaccgcggtg acgagcacgg
     7681 tggcgccgcc taaacgctcg ctgccgttcg gcgacgacga aatccccttc gcccccaact
     7741 tcgattgagt atcggaggtt cggagatggg tgagcacgaa ccctgcatcg gtgcaacttc
     7801 ggagtggttt acaccgccgg agtatttcca ggcgctcgat ctcatcttcg atctggatcc
     7861 ctgctcaccg ggtcccggcc actgggtgcc cgcggagaaa gtctacacca tcgaagacga
     7921 tggcctacgc cagccttgat cgagcacatc aaggcgctcg agaagaaata cccagggctc
     7981 gaccgcatct cggtaagcct ctcggtcggc gtgccggaga aggttgcctt ggagcagctc
     8041 gatcggttcg cgaaagaggt gatgcctgca ttcaaagaag ccaatcgtcg gtgctgaccc
     8101 gcgcaaccgt cacatcctga gcccgccgcc gcgacacctg gcatcaggtg caaaccgacg
     8161 catccgtcac tatccgcccg ctggctagcg gtccggaccg cgctattcgg gccattgcgg
     8221 aatggtagtg cctcgccacc gagcgacgct taaccgacca gctggaagcg ctcggactcc
     8281 ggaggcttgg cccagagccg gtcggcctcg ctcatcgcca tcgttcgttg cggactgacc
     8341 ccccaggctt cgtagatgtc gttggtcgtc cacacgacca gggttgtgat tttcgcgggg
     8401 tccgcgagtg cgatgagggt ttgacggctc acaaagcccg gcgccttact ctgtgcatcg
     8461 ccattgcgat ccaacagctt tcg
//
LOCUS       DATLJT010001510         6103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1750824,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001510 DATLJT010000000
VERSION     DATLJT010001510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750824"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VM910_34580"
     CDS             <1..969
                     /locus_tag="VM910_34580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI67672.1"
                     /translation="SALINEIGFAENDRRLAADALAAAETAMAETDRAAKASLEALSS
                     AREATARAEERMEGARRRLADVEREIRDMLEIEPEGVAALAEIEPGAELPPLAEIEEN
                     LEKLRRDRERLGAVNLRAEEELHEVEAQHTKLTTERDDLVEAIKRLRQGIQSLNREAR
                     ERLLASFEIVNGHFKRLFVELFGGGEAALHLIESDDPLEAGLEIIAKPPGKKPQTLSL
                     LSGGEQALTALALIFAVFLTNPSPICVLDEVDAPLDDHNVERFCSLLHEMTGSTETRF
                     IIITHNPITMARMNRLFGVTMAERGVSQLVSVALEEAVKILDQNVA"
     gene            complement(979..2355)
                     /locus_tag="VM910_34585"
     CDS             complement(979..2355)
                     /locus_tag="VM910_34585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HVI67673.1"
                     /translation="MNPQPLPPGPDPRVQITEAYARLVARDAFMWAWPLANVYNRRLT
                     FKDLPEAGLMGGIAPVGPPNRLAMLTDYVQPEERLVACPNQDVVYGFGLLALDQSPLV
                     IQVPDFGDRFWVYQIVDLRTDSFADLGKMYGSRPGFYLLVGPNWKGKVPKGITAVFRS
                     TTNTGVVIPRVFQDDTPEDKKAVQAVIDGIELYPLADFDGKMKRRDWAKLPKFPSQSQ
                     GDAETKWVPPEKIFDELPAVLKDAPPLPGEAARYEQVLAVIDAANKNPDLKKAMIDEA
                     GKADEDLVDPLFEFRNWGIKLPNNWSTVDNGAAFGTDYFTRTAVAKSNIFVNKRNETK
                     YFYQDLDASGGRLNGNNRYTVTFAKGQLPPVRGFWSLTLYNQHHFFSPNDLKRYSLGT
                     KNKNLKYNPDGSLTLAVQADPPADDQRSNWLPAPKGEDFSLYVRSYWPKAEIIDGSWT
                     PPPVVRAN"
     gene            complement(2738..3502)
                     /locus_tag="VM910_34590"
     CDS             complement(2738..3502)
                     /locus_tag="VM910_34590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI67674.1"
                     /translation="MKKFWLGTVGVLALGMAAPASAADLAARPYTKAPPPPVVAPIYD
                     WSGFYIGANGGWGSAHNCWDVTPFGGVLFNDGCVNRSGGVIGGQVGYRWQANQFVFGL
                     EGQGDWASLRGSHVSLLDPTLTERTRVDGIGLFTGQIGYAWNAALLYLKGGAAVTSNR
                     FDLLGTLTGLGLASVDTTRWGGTVGVGFEYGFAPNWSLAFEYDHLFMGNANDTFNNTV
                     LVGHPFFNALNRISEDVDMVTVRFNYRFGGYGVSRY"
     gene            4027..5001
                     /locus_tag="VM910_34595"
     CDS             4027..5001
                     /locus_tag="VM910_34595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HVI67675.1"
                     /translation="MTPPSLPAELIAALDARLQGVSRHELATRAEKISKTYREGGRSM
                     TITSATDALAYALVRMPATYAAVAACLNAVQEIMPDFAPRDLLDVGAGPGTASFAAAD
                     AFSSLGNFTLLDANPALRELALGLLRDNKRLHPLKYEHGQVQALLADREAADLVVASY
                     VIGELGEAERRTLAELLWDRTRDTLLIVEPGTPAGYARILAARAQLIASGAHVVAPCP
                     HDGECPLAAPDWCHFAQRLPRSRAHKAVKAADLPFEDEKLTYIALSRKPAPQHFARLL
                     APPHLNKVEATAKLCTADGLVLAKVPRRDKSAYAAARRWRWGDLVQSV"
     gene            complement(5036..5188)
                     /locus_tag="VM910_34600"
     CDS             complement(5036..5188)
                     /locus_tag="VM910_34600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67676.1"
                     /translation="MTTLVRARSYVSGTDIETEPLKIIAMFCGAGLLVSLLFASYGLD
                     LSAGFF"
     gene            5642..>6103
                     /locus_tag="VM910_34605"
     CDS             5642..>6103
                     /locus_tag="VM910_34605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI67677.1"
                     /translation="MVDRPTDEIGQTADDLDFVLIDEVRPPPRIMMARERLLLCRLYV
                     GLIRTLNDDYGAAFAANSDSCTLRTIGIYVLLRTLMCTPVRASTIAQALKLPRVTVNR
                     RLQEMMKYGYVERVGNAYRMTDKVNIPDLQDRLQRRIDMIVETAGELSKLRA"
BASE COUNT         1209 a         1912 c         1830 g         1152 t
ORIGIN      
        1 agcgccctga tcaacgagat cggctttgcc gagaacgacc gccgcctcgc cgccgacgcg
       61 ctcgccgccg ccgaaaccgc catggcggaa accgatcgcg ccgccaaggc gtcgctcgag
      121 gcgctgtcga gcgcgcgcga ggccaccgcc cgagccgaag agcgcatgga gggcgccagg
      181 cgccggcttg ccgatgtcga gcgcgaaatc cgtgacatgc tcgaaatcga gccggaaggg
      241 gtcgccgcgc ttgccgagat cgagccgggc gcggagctgc ctccgcttgc cgagatcgag
      301 gagaacctcg agaagctccg acgcgatcgc gagcggctgg gcgcggtcaa tctgcgcgcc
      361 gaggaagagc tgcacgaggt cgaagcccag cacaccaaat tgaccaccga gcgcgacgat
      421 ctcgtggaag cgatcaagcg cctgcgccag ggcatccaga gtctcaaccg cgaggcacgc
      481 gagcggctgc tcgcctcttt cgagatcgtg aacggccatt tcaagcgcct gttcgtcgaa
      541 ctgttcggcg gcggcgaggc tgccctgcat ctgatcgaaa gcgacgatcc gctggaagcc
      601 gggctcgaga tcatcgcgaa gccgccgggc aagaagccgc agacgctctc gctgctctcg
      661 ggcggcgagc aggcgctgac ggcgctcgcg ctgatcttcg cggtgttcct gaccaacccc
      721 tcgccgatct gcgtgctgga cgaggtggac gccccgctcg acgaccacaa tgtggagcgc
      781 ttctgcagcc tgttgcatga gatgacgggt tcgaccgaaa cgcgcttcat catcatcacg
      841 cacaacccga tcaccatggc gcggatgaac cggctgttcg gcgtcaccat ggccgagcgc
      901 ggcgtctcgc aactggtctc ggtcgcgctt gaggaggccg tgaagatcct cgaccagaac
      961 gtggcgtgag ttcctaattt agttcgcgcg gacgacgggc ggcggcgtcc acgaaccgtc
     1021 gatgatctcc gcctttggcc aataggaacg gacgtagagc gagaaatcct cgcccttggg
     1081 cgccggcaac cagttactgc gctggtcgtc cgccggcgga tcggcctgga cggcaagggt
     1141 gagggagcca tcagggttgt atttcaggtt cttgttcttc gtccccagcg aatagcgctt
     1201 gaggtcgttc gggctgaaga agtggtgttg gttgtagagc gtcaacgacc agaagccgcg
     1261 aaccggtgga agctgtccct tggcgaaggt tacggtgtag cggttgttgc cgttgagacg
     1321 gccgccactc gcatcgaggt cctggtagaa atatttcgtc tcgttccgtt tgttgacgaa
     1381 gatgttcgac ttcgcaacgg cggtccgggt gaagtagtcg gttccgaaag cggcgccgtt
     1441 atcgacggtg ctccagttgt tcgggagctt tattccccaa ttccgaaact cgaacagcgg
     1501 atcgaccaga tcctcatcgg ccttcccggc ttcgtcgatc attgccttct tcaaatcagg
     1561 attcttgttg gcggcgtcga tgacggccaa aacctgctcg taacgcgccg cttcacccgg
     1621 aagcggaggc gcgtccttca gcacggccgg cagctcatcg aatatctttt ccggcggcac
     1681 ccatttggtt tcggcgtcgc cttgcgattg cgacgggaat tttggcagtt tcgcccagtc
     1741 gcgccgcttc atcttcccgt caaaatccgc gagtgggtaa agctcgatgc cgtcgatgac
     1801 ggcctggacc gccttcttgt cctccggcgt gtcgtcctgg aatacgcgcg ggatcacgac
     1861 accggtgttc gtcgttgagc gaaatacagc cgtgatgcct ttcggcactt tgcctttcca
     1921 attcggaccg accagcagat agaagccggg cctggagccg tacatcttgc ccagatcggc
     1981 aaagctgtcg gtgcgcagat cgacgatctg atagacccag aagcgatcgc cgaagtcggg
     2041 tacctggatc accaaagggc tttgatcgag tgcaagaagg ccgaagccgt acacgacgtc
     2101 ctgattggga cacgcgacca gccgctcttc cggctgaacg tagtccgtca gcatggcgag
     2161 gcggttcggt ggaccgaccg gcgcgatgcc gcccatcaat cccgcctctg gcaggtcctt
     2221 gaaggtcaat cggcggttgt agacgttcgc cagtggccac gcccacatga acgcatcgcg
     2281 cgcgaccagt cgtgcatagg cctcggtaat ctgcacgcgc gggtccgggc ctggcggcag
     2341 aggttgcgga ttcaactctt gggctcgagc gagagtcgca agacttgcac ttgccagcag
     2401 cgaacatgtc accatcgaaa caaaaacacg gttcatggct tgatttcctc gcttgcgaga
     2461 cgtgatttcg atgtcgagcc tcagtcgaca cagggatcga cggaaggagt cgcacagtcc
     2521 cgttcgtcgg gcgatcgcga accacgaact cggtgcatgg cgcgtaacta agccaagtgg
     2581 cgtgaagcgc gctattggac cgaggtgcaa catcgccccg cgcgggctgc tccgggttga
     2641 gcaactcgcg acggcgctgc aggagtcctt ggactcatga acaaaaacaa aaaggccccg
     2701 gacgagccgg ggccgaagtt tgccgaagct ttgaaaatca gtatctcgaa acgccgtagc
     2761 cgccaaagcg gtagttgaag cgcaccgtga ccatatcgac gtcttcgctg atccgattga
     2821 gcgcattgaa gaagggatga cctacgagca ccgtgttgtt gaacgtgtcg ttggcgttac
     2881 ccatgaacag gtggtcgtat tcgaaggcga gcgaccagtt cggcgcaaag ccgtattcga
     2941 atccgacgcc cacggtgccg ccccagcggg tggtgtctac cgaggcgaga ccaagtccgg
     3001 ttaacgtgcc aagcaggtca aagcgattgc tcgtgaccgc cgcgccgccc ttcaggtaaa
     3061 gcagtgccgc gttccatgca tagccgattt ggccggtgaa aaggcctatg ccatcgactc
     3121 tggtccgttc cgtgagcgtt gggtctaaca ggctgacatg cgaacctctg aggctcgccc
     3181 agtcgccctg gccttccaag ccgaatacga actggttggc ttgccagcgg tagccgacct
     3241 gtccgccgat cacaccaccg gaccgattga cgcatccgtc attgaacagc acgccgccga
     3301 atggagtcac gtcccaacaa ttgtgggcgc ttccccagcc gccgttcgcg ccgatgtaga
     3361 aaccgctcca gtcgtaaatc ggagccacca ccggcggcgg cggcgccttg gtgtacggtc
     3421 ttgccgccaa atctgcggcc gacgcggggg cggccattcc gagtgcgagt acaccaacag
     3481 tacccagcca aaacttcttc atcttgcttt ctccatccag tcgcttgtga cttgtccccc
     3541 attgcgaccg tcgattcatt gttcttgatt ctaggtccca cttcccgccg caacactagg
     3601 cgattggccc ccggattact gtgccgcatc ggcaactatc cgtttttcaa ctgtgacgag
     3661 aatgcgtcct cgggaactcc cggaagtcaa taccgttggt ggttccagtg gttaggacgg
     3721 gttccaacga ccgctatgag ccaaatctca cgcccagctt actctatcgg cgagtgccgc
     3781 aactcgcgca gcgacgtgca ccacaatgcc acgcccatcg ctttcgccga cttcgatctc
     3841 acgggttaac tcatcccagt agagttcgat gttcgaaaca aatccggaaa gaaaacgaag
     3901 tcgacgtcgc cgtcaccgaa gacctgacag gcgacgagaa cgttgcgaac catgccggtg
     3961 cttccaggat gttgggctct tgctgtcctg gcttcgctcg ggcaccctct ccgacaaaga
     4021 gcagcaatga cacccccttc ccttcccgcc gaactgatcg ccgcgctcga cgccaggctg
     4081 caaggcgtct cgcgccacga attggcgacc cgcgccgaaa agatctcaaa gacctatcgc
     4141 gagggcggcc gctcgatgac gatcacatcc gccaccgacg cgctcgccta tgcactcgtg
     4201 cggatgcctg cgacctatgc cgcggtggcc gcctgcctca atgccgtgca ggagatcatg
     4261 cccgacttcg cgccgcggga cctgctcgat gtcggcgccg gaccgggaac ggcaagtttt
     4321 gccgcggcgg acgcgttttc ctcgctcgga aacttcaccc tgctcgatgc caatcccgcg
     4381 ctgcgcgagc tcgcgctcgg gcttttgcgc gacaataagc gcctgcaccc attgaaatat
     4441 gagcacggcc aggtgcaagc cttgcttgcc gatcgggagg cggctgacct tgtggtcgcg
     4501 agctatgtga tcggcgaact cggcgaagcc gagcgccgta cgcttgccga attgctctgg
     4561 gacaggaccc gcgacacgct tctgatcgtc gaacccggca cgcctgcagg atacgcgcgc
     4621 atcctggctg cgcgtgcgca gttgatcgca tcaggcgcgc atgttgtagc cccctgcccg
     4681 catgacggag aatgcccgct cgccgcgccc gactggtgtc acttcgccca acgcctgccg
     4741 cgctcgcgtg cacacaaggc ggtcaaggcc gcggacttgc cgttcgagga cgagaagctc
     4801 acttatatcg cgctgtcacg gaagcccgcg ccacaacatt tcgcgcggct gctggcgcca
     4861 ccgcacctca acaaggtcga ggcgacggcc aaactctgca cggccgacgg acttgtgctc
     4921 gcaaaagttc ctcgccgcga caaatccgcc tatgccgcgg cgcgccgctg gcgctggggc
     4981 gacctggtgc agtcggtttg aagtccccgt acaactgcgc gcggccaccg ggccgttaga
     5041 agaagcccgc gctcagatcc aggccatagc ttgcgaagag cagcgagacg agcaaaccgg
     5101 caccacaaaa catggcgatt atcttgagtg gttcagtctc aatatccgtt cctgaaacgt
     5161 aggaacgcgc gcgcacaagc gtagtcattt gcgacctccg aatagaacaa cctccgttga
     5221 ggcgaacctt ttgatcgtga tcgcgattcg tttccgccta tcttcgcccc ggagaaaaat
     5281 agcttcgcag tcgtttcgtg gaaccctgcc aacgaagtcg gctcacgcat ctgcgagccg
     5341 ggagtgaccg tggtcctgcc gcgcggcttg gtctgatgga cggccgcacc cttgatttgg
     5401 atcaaacgtg tcactcgatg cggcgatagt cacaaaatga ctgcctgccg gattgcgacg
     5461 acctctgtgc gatgttcgaa aacatgcaat cggtgctagc tgaccatctt tacgtcagca
     5521 agccgcacgg aaagcgactt atgatgcgag acgcaatggc acgacacctg gagtcatgag
     5581 atccaaggtc ggccgtccgc ctgccgaacc tgagagacat atgccaacag cgagggttgc
     5641 catggttgac cgcccgaccg acgagatcgg ccagaccgcc gacgatctcg attttgtcct
     5701 gatcgacgaa gtccggccgc cgccacggat catgatggcg cgtgagcgat tgctgctttg
     5761 ccgactgtat gtgggtctga tccgcaccct caacgacgac tatggggccg cgtttgcggc
     5821 caacagcgat agctgcacac tgcgtacgat cggtatctat gtccttcttc gcaccctgat
     5881 gtgcacgccg gtccgcgcca gtacaattgc ccaagcgctc aagctgccgc gagtgacggt
     5941 caacaggcga ctgcaggaaa tgatgaaata cggctatgtc gagcgcgtcg gcaatgcgta
     6001 ccgcatgacg gacaaggtca acatcccgga tttgcaggac aggcttcaac gccgcatcga
     6061 tatgatcgtg gagaccgcgg gcgaattgtc aaaattgcgc gct
//
LOCUS       DATLJT010001511         1527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3818552,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001511 DATLJT010000000
VERSION     DATLJT010001511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3818552"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..612
                     /locus_tag="VM910_34610"
     CDS             232..612
                     /locus_tag="VM910_34610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67678.1"
                     /translation="MVRDTVWELAWRGRRKSWHNVPGQSVLCIGCLEKRIGRSDFTDA
                     PVNNPDKGEISDRMRARLAATESVERAGRRYAASIGEVYVEDPFKRPPHQGGYAHITS
                     EEWAEFDRRKADWEGRRRAFLQRM"
     gene            1048..>1527
                     /locus_tag="VM910_34615"
     CDS             1048..>1527
                     /locus_tag="VM910_34615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67679.1"
                     /translation="MASHIGRRKFLATLGGAAAAWPLAARAQQATMPVIGVLSAEWPN
                     LFSDRLRAFHDGLRETGYVEGRNLAIEYRWAEGRNDRLPELAAELVRRQVTVIVSTST
                     PAVLAARAATTTVPIVFFVAVDPIQLGLVTSLSRPGGNLTGVVTLNVEVAAKRLQLLH
                     "
BASE COUNT          281 a          443 c          515 g          288 t
ORIGIN      
        1 cggctactca agtgccagtg tgcgacgtgc ggctacaccg ttcgcgtcac acgcaagtgg
       61 ctcggcctcg cggggccgcc gatctgtccg acggacaatg ttcggatgat gttggtcggc
      121 gcgccggcca cgccgttggc ggcgtaggag gctgcgataa tgatgtggcc cacgatcgtg
      181 actcggtgct gcggctgtgg cgtcggcacc aacgtgctcg gtgagtggta catggtgcgc
      241 gataccgtgt gggagctggc ctggcgcggc cgccgcaaga gctggcacaa cgttcccggc
      301 cagtcggtgt tgtgcattgg gtgtctcgaa aagcggatcg gccgcagcga tttcaccgac
      361 gcgccagtga acaatccaga caagggggaa atctccgatc gcatgcgcgc tcgcttggcc
      421 gcgaccgaat cggttgagcg cgcgggccga cgttatgcag cgtcgattgg tgaggtgtac
      481 gtcgaggacc cgtttaaacg gccgccgcat caaggcgggt atgcgcacat cacctcggaa
      541 gagtgggccg agttcgatcg gcgaaaggcg gactgggaag gtcgtcggcg ggcgtttttg
      601 cagcgaatgt gagcggccga ccgaggcggc tcaagcgaca aaaggctttt tcggcaagtg
      661 tcaaaaccgg aacagcttga aattccggta ggcctcccgc gcgaacgcgc gttcgtctgg
      721 catcatcggc ggcatgggaa ttccgctgag gcccgcctca gacgagatta tccgcatcga
      781 gcgtcgtgct ctgctgcggg ccgtgaccgc gaaggtgctt cgcgaaacca atcgagaggt
      841 agttcgtact cgttgacgcg cagatcggat gtccgatttg gggagcaaac cggtaaaaca
      901 aatgctcgtt cagagcttta ccggttgacc tcccgtggtc attgggaagc attcattcga
      961 tttgcctatc ggccttagtc gcacaccgcg cgatcgaagc gtggtatcat tccccattgc
     1021 atgaatgacc cccaaccgga gggtcagatg gcaagccaca tcggacgccg aaaattctta
     1081 gccacgctcg gcggcgcggc ggcagcgtgg ccgctcgcgg cgcgcgcgca gcaggccacg
     1141 atgccggtga tcggggtgct cagcgccgaa tggcccaacc tgtttagcga ccgtttgcgc
     1201 gcgtttcacg atggactgcg ggaaaccggc tatgtcgagg gccggaattt ggcgatcgag
     1261 tatcgctggg cggagggccg aaacgatcgc ttgccggaac tggcggccga attggttcgt
     1321 cgtcaggtga ccgtcattgt ctcgacgagc actcctgcgg tgctcgcggc acgggcggca
     1381 accaccacgg ttccaatcgt gttcttcgtc gcagtggacc cgatccaact tggtctggtc
     1441 accagcctca gccgaccggg aggcaatctc acgggcgtgg tcaccttaaa cgtcgaggta
     1501 gccgcgaagc ggctgcagct gctgcac
//
LOCUS       DATLJT010001512         1591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3357235,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001512 DATLJT010000000
VERSION     DATLJT010001512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357235"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VM910_34620"
     CDS             complement(<1..694)
                     /locus_tag="VM910_34620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVI67680.1"
                     /translation="MTPTLTERIIATVRVSDVPNLIWSDRGRAPSGYAKGMAVAFARV
                     YAKWKIGNSAALVMAAANSGDDTRDALSWHNSRFQPTAMDNTKAGADTLRHLFVLLFG
                     LGMRESRGRYCEGRDRSARNISADSAEAGLFQMSWDAHTADAEILRLFGEYRASQDDG
                     LLAIFREGVTAHQYDLESYGSGDGRAFQDLCKSKPLFAVECAAVGLRTIRRHWGPINR
                     HEAVIAPSVDRLF"
     gene            complement(871..1173)
                     /locus_tag="VM910_34625"
     CDS             complement(871..1173)
                     /locus_tag="VM910_34625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67681.1"
                     /translation="MKRRFAVAVQNMTLTEDTAFVEGIRTKNLGWWHWIPGFWLITTK
                     SGGVSTEDIRNQLRKITGRKDIMVLEIQKPMTWSGYGPDTRERDMFKWIRGTWAED"
     gene            complement(1170..>1591)
                     /locus_tag="VM910_34630"
     CDS             complement(1170..>1591)
                     /locus_tag="VM910_34630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67682.1"
                     /translation="CRRPNAVPSAKILTMPDLLRLVSALQEQMNATLSRSDALKPLAW
                     LIVTLTTTSVLLAYVAGPVWLLIVAAGLLAAATALYFIAFSFCLIKDRDALRSETYSL
                     HKIAIEHGLTEDSITGVITVGAEDRRLLTQHKSENQP"
BASE COUNT          317 a          495 c          439 g          340 t
ORIGIN      
        1 cgaataaacg atcgacgctc ggcgcgatca cggcctcgtg ccgattgatc ggcccccagt
       61 ggcgccgaat ggtgcgcaat ccgactgccg cgcattcaac cgcgaacagc ggctttgatt
      121 tgcataagtc ctgaaacgcc cggccgtcac ccgacccata gctttcaaga tcgtattgat
      181 gcgccgtcac cccctcgcgg aagatcgcga gcaggccgtc gtcctgcgat gcgcggtatt
      241 ccccgaacag ccgcagaatt tccgcgtcgg cggtatgcgc gtcccagctc atctggaaca
      301 atccggcttc cgccgaatcg gcactgatat ttcgtgccga gcggtcacgg ccctcgcaat
      361 agcgtcctct gctttcgcgc atgccaagcc cgaacagtag cacgaagaga tggcgcagcg
      421 tgtcggcgcc agccttggtg ttgtccatgg ccgtcggctg gaaccgcgaa ttatgccaag
      481 acagggcatc gcgagtgtcg tcaccgctgt tcgctgcggc catcacgagc gcggccgagt
      541 tccctatctt ccatttggca tagacgcgcg cgaacgccac tgccatgccc ttggcgtagc
      601 cgctcggcgc acgcccgcga tcggaccaga tgaggttcgg cacatcggat accctcacgg
      661 ttgcgatgat ccgctcggtc aatgtcggcg tcacatctgc gatcgataac ggtgtatcag
      721 cgtccggcgc tggccggcgc catccgattg acctgatcgc gcagcgtcaa cagacttcgt
      781 gctacccgcc agctcatggc cgttttcctt ctccagaatc acgccccgcg cgtatcgtcg
      841 agccgcgcgc gagctgtcga ggatgcgcca tcaatcttct gcccacgttc cgcgaatcca
      901 cttgaacatg tctcgctctc gcgtatcggg tccataaccc gaccaagtca tgggcttttg
      961 gatctccagc accatgatgt ccttgcgacc ggttatcttc ctcagttgat ttcgtatgtc
     1021 ttcagtgctg acgccgccgg acttggtggt gattagccaa aatccaggaa tccagtgcca
     1081 ccatcccaaa ttttttgtcc tgatcccttc aacgaaggcg gtgtcttcgg tcaaagtcat
     1141 gttttgcacg gccaccgcaa atcgacgttt catggctggt tctcgctctt gtgctgagtc
     1201 agcaagcgtc gatcttcggc tcccacggta atgaccccag tgatgctgtc ttcggtcaaa
     1261 ccgtgttcga ttgctatttt atgaagagag taagtctccg aacgaagagc atctcgatcc
     1321 ttgatgagac aaaaactgaa tgcgatgaaa tacagcgcgg tcgctgccgc cagcaggccg
     1381 gcagccacga tgagcagcca taccgggcca gcgacataag ccagcagaac ggaggtcgtg
     1441 gtcagggtga cgatcaacca agccagcggt ttgagggcat cagaccgtga cagggtggca
     1501 ttcatctgct cctgcaacgc ggatactagt cgcaaaagat cgggcatcgt caaaatcttt
     1561 gcgcttggaa cggcgttagg gcgtcggcat c
//
LOCUS       DATLJT010001513         4493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1059033,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001513 DATLJT010000000
VERSION     DATLJT010001513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059033"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..879
                     /locus_tag="VM910_34635"
                     /pseudo
     CDS             1..879
                     /locus_tag="VM910_34635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673375.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(893..1120)
                     /locus_tag="VM910_34640"
     CDS             complement(893..1120)
                     /locus_tag="VM910_34640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67683.1"
                     /translation="MFLSCMMVPFQATIIPAFRSPAVAVPRSLIDAARIDGLAEWRIP
                     ARRALRLHRISYGAGFTLAKARPIRSEVRVR"
     gene            complement(1232..2059)
                     /locus_tag="VM910_34645"
     CDS             complement(1232..2059)
                     /locus_tag="VM910_34645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI67684.1"
                     /translation="MSPVERKGTVMTRTYFAEGQILFREGDAADGVFRLLSGYVDVLR
                     ELDGDPVLLGTVGAGQFIGEMGVVENRPRSATARAASEVEVEFLNPTEFLDQIAGSPR
                     TARELIQRLSQRLREADDRIVNDEKRSGRAHGDWKDADDQTAVPSVNNAYLAAKNPWL
                     QRQFHTPLGLGDLPFVVGRRPVIGEGPPPWQPNLKLDDTAPFRLSRNHFIIEKRARGY
                     HVRDLRSTLGTIVNGEPIGNHFRTDDAPLRAGENEVIAGGVDSPFAFSVFIGSSSRT"
     gene            2214..2375
                     /locus_tag="VM910_34650"
     CDS             2214..2375
                     /locus_tag="VM910_34650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67685.1"
                     /translation="MVSALNQGSPDRGYGPWEPGLDENGPPHLVRMTAQGEGPGTVQV
                     TARDPAVRG"
     gene            2617..3639
                     /locus_tag="VM910_34655"
     CDS             2617..3639
                     /locus_tag="VM910_34655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI67686.1"
                     /translation="MIKKLWVLTLVGAMTALGGAARAGVTLDKVKAAGQISCGVPTGV
                     AGFGAPDSQGRWAGFDVDICRALSAAIFGVADKVKYVPLTAQQRFTALQSGEVDLLSN
                     NTTDTLQRDTELGLNFAPVVFYDGQGFLVPKKLGVKSAKELNGATICVQPGTTTELNL
                     ADYFRANKMEFKPVVIEKRDEVLSAFFSGRCDVYTNDSSALNADRLSQAPNPDDYVIL
                     PERISKEPLAPVVRHGDDQWDDIVRWVVYALIEAEEKGITSKNVDEKLKSDEPYIKRM
                     LGVTPGMGKALGLDEKWAYNEIKLVGNYGEIFDRTLGKDTELKFERGLNNLWTHGGLM
                     YAMPIR"
     gene            3663..>4493
                     /locus_tag="VM910_34660"
     CDS             3663..>4493
                     /locus_tag="VM910_34660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01726.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI67687.1"
                     /translation="MATDTATASPRPARPSTWHDPRIRGIAYQILFVGIVAAFGAFLI
                     HNTLVNLRRQNIASGLGFLDREAAFGIGESLIAYSPADTYARAFLVGLLNTLYVAGLG
                     IVLATILGTMMGIARLSSNWLIRKLAQVYVETFRNIPLLLQLFFWWALLRESAPGPRQ
                     AWELLPGVLVSNRGVVFPVPVADPVHRWMLLAFALGILAAVGVSRWSKRRQALTGAQF
                     PMGWVGTGLVLGLPSVVFLAAGAPLQLDWPELKGFNFAGGNAVSPEFAALLIGLTVYT
                     G"
BASE COUNT          826 a         1392 c         1402 g          873 t
ORIGIN      
        1 cgtttaccac tcgcgtcggc tcgatcaccg accggtcttg cgtcatggat tgtatgatcg
       61 gtgtgcggac cgtcttccac acggccacgc tgcacaaacc gcatgtcgca acccatcgtc
      121 ggcaggactt cgtcgacacc aacgtcaccg gcacactcaa tctgctggaa gtggccgtgg
      181 cggcgggtgt caggtcgttc gtctatacga gcacgacgag cgtctttggc gacgccctcg
      241 tgccgcccgc cggggctccg gccgcatggg tgaccgagga ggtgacgccc atccccagaa
      301 acatttatgg cgtcaccaag gccgcagcgg aagacctttg ccagatcttc aataggaacc
      361 acggccttgc atccatcgtg ctcagaacat cgcggttctt ccccgaagag gacgacgaca
      421 gcgcagtgcg ggaagcttat gcggacgcca acatcaaggt gaatgaatac ctctaccggc
      481 gtgtggatct cgaagatgtt gtcagtgcgc atctgctcgc cgccgaacga gcgcccgcac
      541 tcggttttcg tcgctacatc atcagtgcga ctacgccctt tgcgcgcagc gacctcccgg
      601 acctgcgggc cgccgccccg cgcgccgcgc gccgtcgcgt tcctttttta tgaggcagaa
      661 tacgcgcgcc ggggctggag gatggtcccg agcatcgatc gcgtctatgt caacgaacgg
      721 gcctgcgatg agctcggctg gcagccgcgc cgcgatttca agttcctcct cgaccgccta
      781 agagccgatg aagacgtcag aagtcctctg gcccggctcg ttgggtcgaa gggatatcat
      841 cccgagactt tcgcagaagg gccgtatcca gtcgaatgat gctctcgccc tatcaacgca
      901 cccgcacttc cgacctgatc gggcgtgctt ttgcgagggt gaaacctgcg ccgtatgaaa
      961 tccggtggag ccttagcgcg cgtcgtgccg ggatgcgcca ctcggcaagg ccgtcgatcc
     1021 gcgccgcgtc gatcagcgag cgcggcaccg cgacggccgg tgatcggaag gcgggaatga
     1081 tcgtcgcttg gaacggcacc atcatacagg aaagaaacaa caccagctcc tagcgcgtca
     1141 ttcatctccc catcgatctt ttcccagcag ctgaaagcct tcggatgttg ccgtcgtcgt
     1201 gtcgctggga ggggacgccg gtagcgctgc attacgtgcg ggaagacgac cctatgaaca
     1261 cggagaaggc aaaaggtgaa tccacgccgc cggcaatcac ttcattctct ccggctcgca
     1321 gcggcgcatc atcggtgcgg aaatgattgc cgatgggttc gccgttgacg atcgttccaa
     1381 gtgtgctgcg gaggtcgcgc acatgatagc ctcgagcgcg cttttcgata atgaagtggt
     1441 tgcgagatag cctgaacggc gcagtatcat cgagcttgag gtttggttgc cacggcggcg
     1501 gcccttcccc aataaccggc cgacgaccaa cgacgaacgg cagatcgcct agaccgagcg
     1561 gagtgtggaa ctgacgctgc aaccacggat tcttggcagc aagatacgcg ttattgactg
     1621 atggaacggc cgtttgatcg tcggcatcct tccagtcccc gtgtgctcgg ccgcttcgct
     1681 tttcatcatt gacgatgcga tcgtcggcct cgcgcagccg ctgactgagg cgctgaatca
     1741 actcgcgagc ggtccgcggc gacccagcga tctggtcgag gaactcggtc gggttgagga
     1801 actccacctc gacctcgctg gcggctcgag cggtcgcact gcgcgggcgg ttttccacga
     1861 cgcccatttc cccgatgaac tgcccggcgc caaccgtgcc gagaaggacg gggtcgccat
     1921 ctagctctcg gagtacgtcg acgtaaccgc tgagtaagcg aaaaacaccg tccgctgcat
     1981 ctccctctcg gaagaggatt tgcccttcgg cgaagtacgt gcgagtcatt acagtccctt
     2041 tcctttcaac cggcgacaag atcccccatc acggcagatt gcgggtgcgc ggatttacga
     2101 actaaaagtc atggcgaacg gaatctcgac actcaagacc tgaaactaca cgagatcctt
     2161 cgtgaccgga tgcttctttg cacggcttcg gtctagattt tgaggaggaa atcgtggttt
     2221 cagcattgaa ccaagggtca cccgatcgcg gctacggccc ctgggagccg gggctcgacg
     2281 aaaatggacc accgcatttg gtgcgaatga cggcccaggg cgagggaccg ggcaccgtgc
     2341 aggtgacggc gcgtgatcca gccgttaggg gctgatatct tggtgttctt cgaactcggc
     2401 ggccgcgagg ccgtctgtcg tgttccgccc cggacggtgc gttagaccgg cgaacgcgtc
     2461 accctgttca tcgatacggc gtgcctgctc tcttttgatc cggtcaccga accggcaata
     2521 taccccactg gtgagcccga ccgcgttgct ggctgcgggg cggctatgat accctgacgt
     2581 gcatcgggag aggccgcggc gttcgggagg cgagacatga tcaagaagct gtgggtgctg
     2641 acgctcgtcg gcgcgatgac cgccctaggc ggtgcggcgc gggccggggt cacgctcgac
     2701 aaggtcaagg cggccgggca gatcagctgc ggcgtgccga ccggcgttgc cgggtttggc
     2761 gcacccgaca gccagggccg ctgggccggc ttcgacgtcg atatctgccg ggcattgtcg
     2821 gccgcaatat tcggcgtcgc cgacaaagtg aaatacgtgc cgctgactgc gcagcagcgt
     2881 ttcaccgctc tgcagtcggg cgaagtcgac ctgctgtcga acaacaccac cgacacattg
     2941 cagcgcgaca ccgagctggg gctgaatttc gcgccggtcg tgttctatga cggccagggg
     3001 tttctcgtgc cgaaaaagct cggcgtcaag agcgccaagg agctgaacgg cgccacgatc
     3061 tgcgtgcagc cgggcacgac gaccgagctg aacctcgccg attacttccg cgccaacaag
     3121 atggagttca agccggtcgt catcgagaag cgcgacgagg tgctgagcgc cttcttctcc
     3181 gggcgctgcg atgtctacac caacgattcg tcggcgctca acgccgaccg actgagccag
     3241 gcgccgaacc cggacgatta cgtgatcctg cccgagcgca tctcgaagga gccgctcgcg
     3301 ccggtcgttc gccacggcga cgatcaatgg gacgacatcg tccgctgggt ggtctatgcc
     3361 ctgatcgagg ccgaagagaa agggatcacg tcgaaaaacg tcgacgagaa gctgaagagc
     3421 gacgagcctt acatcaagcg tatgctcggc gtcactcccg gcatgggcaa ggcgctcggg
     3481 ctcgacgaga aatgggccta taacgagatc aagctggtcg gcaattacgg tgagatcttt
     3541 gatcgcacgc tgggcaagga caccgagctc aaattcgagc gcggcctcaa caatctgtgg
     3601 acccacggcg ggctgatgta cgcaatgccg atccgctgag tagagcggct tcggggtcgg
     3661 gcatggcgac cgacacggcg accgcctctc cgcggcccgc tcggccatct acctggcacg
     3721 accctcgtat ccgaggtatc gcctatcaga tcttgttcgt cgggatcgtc gcagcgtttg
     3781 gcgctttttt gatacacaat acgcttgtca acctgcgccg ccagaacatc gcgagcggct
     3841 tgggctttct tgaccgcgaa gccgcctttg gcatcggaga gtcgctgatc gcctattcgc
     3901 cggccgatac ctatgcgcgc gccttcctcg tcgggctgtt gaacacgctt tatgtcgcgg
     3961 ggctcggcat cgtcttggcg acaatcctcg gcacgatgat ggggattgcg cggctgtcga
     4021 gcaactggtt gatccgcaag ctcgcccagg tctatgtcga aaccttccgc aatatcccgc
     4081 tgttgctgca gctgttcttc tggtgggcgt tactgcgcga gagcgcgccg gggccgcgcc
     4141 aagcctggga gctgttgcca ggcgtcctcg ttagcaatcg cggcgtcgtt ttcccggtgc
     4201 cggtcgccga tccggtgcat cgctggatgt tgctggcgtt tgccctcggc atcctcgcgg
     4261 cagtcggggt aagtcgctgg tcaaagcgcc gtcaggcttt gaccggtgcg caatttccaa
     4321 tggggtgggt ggggactggt ctcgttctcg gtctaccgtc tgtggtgttc ctcgccgccg
     4381 gcgcaccgct ccaactcgat tggccggagc tcaaagggtt caactttgcc ggcggaaacg
     4441 cggtttcccc cgaattcgcc gcgctgctga tcgggctcac cgtctatacc ggc
//
LOCUS       DATLJT010001514         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4048591,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001514 DATLJT010000000
VERSION     DATLJT010001514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4048591"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..242)
                     /locus_tag="VM910_34665"
     CDS             complement(87..242)
                     /locus_tag="VM910_34665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HVI67688.1"
                     /translation="MLVGVLVTFLVVILILYLINMLPLDGRARQICRVIVIILGVLSL
                     LKYLAVY"
     gene            complement(381..1016)
                     /locus_tag="VM910_34670"
     CDS             complement(381..1016)
                     /locus_tag="VM910_34670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HVI67689.1"
                     /translation="MNGRTVTLTTCVVLALSAFGLARSAQAGDLFSELFGAFRRSAPP
                     PMPLPFANSFAPAFEVPRARSGFGGGQAWCVRTCDGRYFPVQGPDRESRASSCSNFCP
                     AANTEVVYGGDIDSAVTDGGKTYSDLPNAFRYRDELVAGCTCNGKEPAGLAQVPVQSD
                     PTLRRGDIVASENGLVVTRRAADRHAAANFSPVPESVRTRYGRAPIVASGR"
     gene            1225..1596
                     /locus_tag="VM910_34675"
     CDS             1225..1596
                     /locus_tag="VM910_34675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HVI67690.1"
                     /translation="MSITNGETAMKDMASQATYQRLEKDISAVKNDIAALTDQITDAI
                     NTLANAAKKQARRGYKDARANVDSAIDDASARGGAMLDAAQDAAVSLEETLEDVITQR
                     PLAAVGLALGIGFLIGVTWRR"
     gene            1752..2198
                     /locus_tag="VM910_34680"
     CDS             1752..2198
                     /locus_tag="VM910_34680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67691.1"
                     /translation="MSLRLIDDFKASTHAAVLQTELAAAAVFAFLVAIAFLCAACFII
                     VREQYGPVEACLAGAGLFLVVALIVIGIYAARRRQIEARAAARAKSTAQTLLSDPALL
                     AAGIQIVRMVGVKRLIPILAVGGLALGLMAGRSQANAPAGEQAPAE"
     gene            complement(2217..2606)
                     /locus_tag="VM910_34685"
     CDS             complement(2217..2606)
                     /locus_tag="VM910_34685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67692.1"
                     /translation="MSYTVNGILGVVAVVATFGAVQFASGHDLTVGMRSSDTPLQEEV
                     NRTVKADRAAVVAAMPAQARTISIRLERLPNTSIVVRLPAAREARSTGPALFIKSGER
                     AAAIACEPMVSVLTEVVRQLQPGRCVT"
BASE COUNT          617 a          948 c          978 g          527 t
ORIGIN      
        1 gacgactggt tcaagacgct gaagtagttc gtatcaccgc aacaaaagcc ccggccaaag
       61 ccggggcttt ttcattgagg cgaacctcag tacaccgcga gatatttcaa cagcgagagc
      121 acgccgagga tgatgacgat cacgcggcaa atctgcctgg cgcggccgtc cagcggcaac
      181 atgttgatga gatagagaat gaggatgacc acgagaaacg tgacgagaac gccgaccagc
      241 atcaaagacc ccttaaggct tcaaaacgaa acgccaatgg cacctcaacg cgccggccgc
      301 ccgccgggtt ccactctcgg gaaccatggt cgaaaaagct ccgctaagtg cctggaaaag
      361 cgcagcaacg gtcagctggc ctatcgcccc gatgccacga tgggagctcg cccatagcgc
      421 gtgcgcaccg actccggcac gggcgagaag ttcgctgccg catgcctgtc ggcggcgcgc
      481 ctggtgacca cgagtccgtt ctcgctggcg acgatgtcgc ccctgcgcaa ggtcggatcg
      541 ctttggacag ggacctgcgc gagcccggcg ggctccttgc cgttgcaagt gcaccccgca
      601 accagttcgt cgcgataacg gaacgcgttg ggcaaatccg aataggtctt tccgccgtcg
      661 gtcaccgcac tgtcgatatc gccgccatag accacctcgg tgttggctgc cgggcagaaa
      721 ttgctgcacg aggacgcgcg gctttcccgg tcgggcccct gcaccgggaa atagcggccg
      781 tcgcaggtcc gcacgcacca ggcctgaccg ccgccgaagc ctgagcgcgc ccgcggcact
      841 tcaaaggccg gagcaaagga gttggcaaac ggcaacggca tcggcggcgg cgcggatcga
      901 cggaaggcgc cgaacaactc ggaaaagaga tccccggctt gcgccgatcg cgcaaggcca
      961 aaggcggaaa gcgccaaaac gacgcaggtg gtcagggtga ccgttcgccc gttcatggcg
     1021 gcctccgaca cgtttcgggt caagacgttg caggatccag gacgttgcgt gtctcggaca
     1081 ttgcagtggc gcaccgacaa acgccgtcga cgcttgcgcg gttcccctga aacggagtcg
     1141 aagcagccgc ggcaaaggtt caacacgccg gaacttccga caccaccaaa cgtttccacc
     1201 gcgaatgaat tcgaggagcg ttcgatgtcg ataacgaatg gcgaaaccgc gatgaaggac
     1261 atggcgagcc aagccacata tcaacgcctt gagaaggata tatcggccgt gaaaaacgat
     1321 atcgctgccc tgaccgacca gattaccgat gcgatcaaca cccttgcgaa cgcagccaaa
     1381 aagcaggcgc ggcgcgggta caaggacgct cgcgcaaacg tggattcggc gatcgacgac
     1441 gcttcggcgc gcggcggcgc catgcttgac gcggcacagg acgctgccgt gtcgctcgag
     1501 gagacgctgg aggatgtcat cacgcagcgg ccgctcgcgg cggtcgggct ggcgctcgga
     1561 attggttttc tgatcggggt gacgtggcga cggtaatttg cgcgggtcag aacgaccagg
     1621 cacttgtgtc ttgaggagcc aacgacggag agcagcgatg gtctccaaaa catccaatct
     1681 tctgcaccag tggcgacgat ggtgggtaac ggttgccttc gtggacgccc tctacaaatc
     1741 gaggcgggca gatgtccctg cggctaattg atgacttcaa ggcttctacc catgcggcgg
     1801 tgctccagac ggagctagcg gcggcagccg ttttcgcctt cctggtcgcg atcgcctttc
     1861 tgtgcgccgc atgcttcatc atcgtgcgcg agcaatacgg gccggtcgag gcttgcctgg
     1921 cgggcgccgg gctgtttctc gttgtcgcat tgatcgtcat cggcatctac gcggcacgca
     1981 ggcgccagat cgaggcgcgc gccgcggcgc gtgcgaagtc caccgcgcaa accctgctgt
     2041 ccgaccccgc tttgctcgcg gccggtatcc agatcgtccg gatggtgggc gtcaagcgac
     2101 tgatcccgat cctcgcggtc ggcggattgg cgctcgggct gatggctggc cgcagtcaag
     2161 cgaacgcccc tgctggcgag caggcaccgg cggaatagaa caatcagtca agaaggtcag
     2221 gtgacgcagc ggcccggctg gagttgcctg acgacctcgg tcagcacgct gaccatgggc
     2281 tcgcaggcga tggcggctgc gcgctcgccg gacttgatga agagagcggg tcccgtgctg
     2341 cgcgcttccc gcgctgccgg gaggcgcacc acgatcgagg tattgggtag tcgctccagc
     2401 cgaatggaga tcgtgcgggc ttgcgccggc atcgcggcga ccacagccgc ccggtctgcc
     2461 ttgaccgtgc gattgacttc ctcctgcaag ggcgtgtcag agcttcgcat ccccacggtc
     2521 aggtcgtggc cggaagcaaa ctgcaccgca ccgaacgtcg ccacgacggc gaccacaccc
     2581 aaaattccgt ttactgtgta cgacattact agaccccctc gccccctgtg gcgatcggta
     2641 ctccaacgtt agtcgcggga acccggttcg cggtcgggtc gatcacataa ccgtgtgcaa
     2701 aaaaatttat tgagcgcggg aacgagttcg gcagcccgga gtcttctctg cagccggtta
     2761 ttccccccct gccccattga ccggcgacgt agggcgcggc tgtggtgatg ccagccgtgc
     2821 cctacttttt tgttctgaaa tcattgacga agttggcgat tttcaatcgg ggtcgcgcgc
     2881 ggcgctgtcc actagctcgg cagcttccgt gcgagttgac ggatcctggc aacttcggcg
     2941 cgcgcctatt gcgggaccga tcgatcggct cggtattgct gtttgggaaa gccaagaccc
     3001 agagcaggac cggagaggag ctggagcggc gatcgaagtt aacccctgga aatgggcaca
     3061 aatcgatagt
//
LOCUS       DATLJT010001515         3637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_370785,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001515 DATLJT010000000
VERSION     DATLJT010001515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370785"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1589)
                     /locus_tag="VM910_34690"
     CDS             complement(225..1589)
                     /locus_tag="VM910_34690"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI67693.1"
                     /translation="MLKAPMLLAAVLVLCVASSADARRRHHHGYYGYGERTQSGFDDW
                     RRARAAQDRDKVPQQTHDQNSQQSHDQDPQQGRDPDSQQNRAQDPGQPRGQDRAQDQN
                     SGQDRTRDSHDRRRARYDDWRRARDERRDWRRSRDDDSRRAREREDWTRRREAERSNA
                     ELRRGRGPFGAVVEKLIRGCGQQGADFGNWPFDALARIAGADEDQRTALTALRDSAKK
                     PAERLAADCPQDVPAAPSTRLEAVEQGIDAMLAAFDRVEPALQAFYGALDDEQKARLY
                     RDMMAAPAAGNAAETTGRRDTREDARQANSSRRHRSRAAYASAGRAGAERAAAARDTS
                     GPGRSAMCEALATVLRGWPVREIERDVRLSSAQRVAFYELVTSSLKAADTLGGACPAE
                     TALTPVGRMEAMRQRLSARARRDRRDPPRARALLRGARPGPEAALRGNELRAVPFPPL
                     SRRI"
     gene            2396..>3637
                     /locus_tag="VM910_34695"
     CDS             2396..>3637
                     /locus_tag="VM910_34695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67694.1"
                     /translation="MPSDPRIGTIRSDCQQSPSGSKYVVAGLSLGSRVQFDSSDYREY
                     KCSPSEQFGGFTWCQKTRQERGFANVTYSILHSQDGTLVYVNRFQEPAFFGRNEADEA
                     IKQYSTRIGAKPQINRLPPRRGIPEGIIATWGGVVLEPLDNDSIKMLAEGRSPKKGYL
                     IDFIGNFARSAKEGLPIYRISGGAGFLWLASFDPIGRGILRFAAVDPSASHASPLPNE
                     ARDGKVQVTPDTHPAGAETASTDANAGRQEAERAAEEAKADAQVARNEAETAKRDAQL
                     AKAEVERLSAEAAKLNSALERLQTEKNEARMMEFVVDGGIIILIALIAIISSILFINR
                     EKAIAGKADGVGPEAKLSGMIGDSPVVETQRASDGDASEPTKASDTILASPETAQFLP
                     NATAPKSQNGSGASDAISTSEE"
BASE COUNT          714 a         1044 c         1158 g          721 t
ORIGIN      
        1 atgtatggtc cggccgtgcg ttgcaagaga actttcatcg agttggcgga tgtgcggtct
       61 tgcatcaatg tatccggcct ttgattggag cctcttgtgc tccgggccat catgggtatc
      121 agcgcgcgtg cgatctcatt accggaaagg cctcaagggg ccatttggag cgattttgcc
      181 aagggtctcc tcccggcccg cattcgcctg agcgtgtcga agacttagat gcgcctggat
      241 aagggcggga agggaacggc cctcaactca ttcccgcgaa gcgcagcttc tggccctggt
      301 cgagcgcctc gtagaagtgc gcgagcgcgg ggcggatcgc ggcggtcgcg gcgcgcacgg
      361 gcggacaagc gctggcgcat cgcctccatg cggccgaccg gagtgagcgc ggtctcggcg
      421 gggcaggcgc ccccgagcgt gtcggcggcc ttgagcgagg aggtgacgag ctcgtagaag
      481 gcgacgcgct gcgccgagct taaacgcacg tcgcgctcga tctcgcgcac cggccaaccg
      541 cggagcaccg tcgcaagcgc ttcgcacatg gcgctccggc cggggccgga ggtatcgcgt
      601 gcggcggccg cgcgctcagc acccgcgcgc ccggcgctgg catacgcggc gcgggagcga
      661 tggcgccggg acgaattcgc ctgacgcgcg tcctcgcggg tgtcgcgccg acccgtcgtc
      721 tccgccgcat ttcccgcggc cggcgccgcc atcatgtcgc ggtagaggcg cgccttctgc
      781 tcgtcgtcca gcgcgccgta gaacgcttgc agcgcgggct cgaccctgtc gaacgccgcg
      841 agcatggcgt cgataccctg ctccaccgcc tcgagccgcg tcgacggcgc cgcgggcacg
      901 tcctgcgggc agtcggcggc gaggcgctcg gccggctttt tggcgctgtc gcgcagcgcc
      961 gtgagcgccg tgcgctggtc ctcgtcggcg ccagcgatgc gcgccagcgc gtcgaacggc
     1021 cagttgccga aatcggcgcc ctgctggccg cagccgcgga tgagcttctc gaccacggcg
     1081 ccaaacggac cgcggccccg gcgcaactcg gcattgctgc gctcggcttc gcgccggcgc
     1141 gtccaatcct cgcgctcgcg ggcgcggcgc gaatcgtcgt cgcgtgagcg gcgccaatca
     1201 cggcgctcat cgcgtgcccg ccgccaatca tcgtagcggg cgcgccggcg atcgtgcgaa
     1261 tcgcgcgtgc ggtcttgtcc gctattttga tcttgcgcgc gatcttggcc gcgcggttgt
     1321 cccggatcct gcgcgcggtt ttgctgtgaa tctgggtcgc ggccttgctg gggatcttgg
     1381 tcgtgacttt gctgcgaatt ttgatcgtga gtttgttgcg gaactttgtc gcgatcttgg
     1441 gccgcgcggg cacgccgcca gtcgtcgaag ccagattggg tacgctcgcc gtagccgtaa
     1501 tagccgtgat gatggcggcg gcgggcgtcg gcgctcgagg ccacgcacaa caccagcacc
     1561 gccgcaagca gcatgggtgc tttcaacatg ggtcacgccc cccgttgatc ctcgcccata
     1621 acaacgcgcc aaacaccgtt tggttcgcca cacacttcgc cacccactcg gcgctttctt
     1681 atggctggag gtttcttacg ggtgggggtc gtcagatggg cgaagtgctt gcagtgccat
     1741 aggtcggcgg ccgcagcgcg ggcaagggcg actgtgcctc gcgctagcga ccagctaacg
     1801 tattcatctc gttccaactt gcccgtcaga gtacaaacaa aggcgtccaa tgacacaatt
     1861 ggcagaggac agcgcgcgcg ccattcttcg ggttttcttt gctcgttcgt tccatgtcga
     1921 tagttgagtg gttggtttgc tgagcgggcg gcaaaagaat tggtcgtgcg cagccccaac
     1981 ctactcaaac cattagggcc gagatgagag gcaggggatg agcggcgttt cccggataag
     2041 ccaacacgtg gctgttggtc aggtgctcaa cctattgagc ggcttgaaaa tttctagcgc
     2101 attagaaaag ccgcttttat gcccgtaact gcagcgcttg ggggctcaag atggggagaa
     2161 agccgtgtca agattgagga tggttgtccc agttctccta ttggccggca cctcgactgc
     2221 gtttgcgaaa actccgaacg atttgaccag gatgctcggc gctccgcagc gtctcagcgt
     2281 ccaagcagca acgcgatccg aatggaataa aatttcgcgc cctgagatcg ggtgcataaa
     2341 cgacgcatta cgccaacacg gtgctaggat tgaaactctg attcaacgag gcgtgatgcc
     2401 gtcggacccg agaattggca ctattagatc ggattgtcag caatcgccat cgggttcgaa
     2461 atatgtggtc gctggtttga gtctcggctc acgggttcag ttcgatagtt cagactatcg
     2521 agaatataag tgcagcccga gcgagcaatt cggtggattt acgtggtgcc aaaagacgcg
     2581 tcaggagcga ggatttgcca acgtcacata ctcaatcctg cactcgcagg atgggacgct
     2641 tgtttacgtc aatcgtttcc aagagccagc tttctttggt cgaaatgaag ccgacgaggc
     2701 cattaagcaa tattccacta ggatcggtgc caagccgcaa ataaataggc tgccgccccg
     2761 acggggtatt ccggaaggca tcattgccac atggggcggg gttgtattag agcccctcga
     2821 caacgacagc atcaagatgt tggcggaagg gagaagtccc aaaaaagggt acttgataga
     2881 cttcattggc aactttgctc ggtctgccaa ggaaggttta ccgatatacc ggatcagcgg
     2941 aggcgcagga ttcctttggc ttgccagctt tgatccgata ggtcgcggta ttctgcggtt
     3001 tgcggcggta gacccttcag cgtctcatgc aagtccgctg ccaaacgagg cacgggatgg
     3061 caaggtgcag gttacgccgg atactcatcc tgcaggagcc gaaactgcga gcacagatgc
     3121 caacgctggg cgtcaggaag ctgaaagggc agcagaagag gcaaaagcgg atgcgcaggt
     3181 tgcgcgtaat gaggccgaga cagcaaaacg cgatgcgcaa ctcgctaagg ctgaggttga
     3241 gagattgagc gcagaagcgg cgaagctgaa ttccgcactt gagcgattac aaacggaaaa
     3301 aaacgaagct cgtatgatgg aatttgttgt tgatggtggg atcatcattt taattgcgct
     3361 tatcgcaatc atctcatcaa tcctctttat aaatcgggag aaagcaatag caggcaaagc
     3421 ggatggggtt ggacctgagg ccaagctgtc agggatgatt ggagattctc cagttgttga
     3481 aacacaacgc gcatcagatg gtgacgcctc ggagcctaca aaggctagcg acactatttt
     3541 ggcttcgccc gaaacagcac aatttttgcc caatgccact gctccaaaaa gccagaacgg
     3601 gagcggtgca tcagatgcaa tttccacttc cgaagag
//
LOCUS       DATLJT010001516         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3819091,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001516 DATLJT010000000
VERSION     DATLJT010001516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3819091"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VM910_34700"
     CDS             complement(<1..429)
                     /locus_tag="VM910_34700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67695.1"
                     /translation="MGVEFDYRPSDVGLVDEDGRQWRDRFSVVELPSRQCVEAPMSCC
                     DETTARVPATDVSRRSLFKGAALVASVIPAARPSAAEAQGKQIKLAYCSQLLCGVPYE
                     VARSGGHFKAHGLDVQLVYTRGGNAAMQALVGGAVDYAATA"
     gene            608..1540
                     /locus_tag="VM910_34705"
     CDS             608..1540
                     /locus_tag="VM910_34705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI67696.1"
                     /translation="MRRLRTFVAVAEMGTVSKAALRLRIGQPALSRQISDLQRELGLR
                     LFDRVGRGLVLTAEGEQFLGDCRAVLGHVDVLGERVELLRRRDRGVLKVAAPPQTIES
                     VLSTFLPRYAERFPNVHVKLTEALGRDQTPMLERGEVHVGIRHDPGDPRFESLVLPAD
                     EVLAACTPSLELGHAGMIDIGRLASYPLLLLESGYSIRRLFNAACRLADVEPNIVLES
                     RAPHTLLALAEAGQGVAIIPSVLRTDRHTLRIVRVTHRRKPLRERYAIQWDKRRPMPP
                     YAESFCEALAKHMRHVLPVTRPSIEQSTAARKQR"
     gene            1741..2157
                     /locus_tag="VM910_34710"
     CDS             1741..2157
                     /locus_tag="VM910_34710"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67697.1"
                     /translation="MPAQPRKVVHPDIKPENTPELASRYLDVPNMPWEPTKFAGIRIK
                     VLYSDDSGITTALFKLDPGAIVPLHEHTALEQTFVLEGTLEDHEGACGPGQFVWRPAG
                     NRHEAVAPNGAVILGFFLKPNRFARGERFFTEDEAQ"
     gene            2289..2711
                     /locus_tag="VM910_34715"
     CDS             2289..2711
                     /locus_tag="VM910_34715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67698.1"
                     /translation="MARETSRAVLGCVLILLLAAVAGCSDFDFWGKPEKANDPNAYPE
                     NYKKDVLTYVKTHPRELLNAREASISTPALKQFGTQSRYFACLRVNGPDWRKEKMLVF
                     YSGEINQFIDAEGDQCGAVAYQPFPELVTEISEFKGKK"
BASE COUNT          546 a          925 c          873 g          524 t
ORIGIN      
        1 cgcggtagcg gcgtaatcga ccgcgccgcc gaccagcgcc tgcatggcgg cgttgccgcc
       61 gcgggtatag acgagctgca cgtcgagccc gtgggccttg aagtgtccgc cggagcgcgc
      121 gacctcgtag ggcacgccgc agagcagctg gctgcaatag gcgagcttga tctgcttgcc
      181 ctgcgcctcg gcggcgctgg gccgcgccgc cggaatgacg cttgcaacca gcgccgcgcc
      241 cttgaacagc gagcgccgcg acacgtcggt ggcggggacg cgggcggtgg tttcatcgca
      301 gcagctcatg ggagcctcca cacattggcg cgagggcagt tcgacgaccg aaaatctatc
      361 gcgccactga cggccgtctt cgtccacgag cccgacatcg ctcggccggt aatcgaattc
      421 gacacccaat caagctcaac ttcgcgtgaa agctggagtt ccagcggtca atcctcgccg
      481 gcatcgcgct cctggctaca atcgtcgacc agcgctcatg gcaagatatg ccgttgatca
      541 gcctgatgtc caatgcatag ttcttctagc atttcatgcc gatctggcat agattgaagg
      601 catggacttg aggcgcctgc gcacctttgt cgccgtagcc gagatgggca cggtgtcgaa
      661 agcagcgctg cgcctgcgca tcggacagcc ggccctttcg cggcaaatca gcgatttaca
      721 gcgcgaactg gggctcaggc tgtttgaccg cgttgggcgc ggcctcgtcc tgaccgcgga
      781 aggcgagcag ttcctcgggg attgccgcgc cgtgctgggt cacgtcgacg tgctcggcga
      841 gcgggtggaa ctactccggc gtagggatcg gggcgtgctg aaggtcgccg cgccgccgca
      901 gacgatcgaa agtgtcctat cgactttcct gccccggtac gccgagcgct ttcccaacgt
      961 gcacgtgaaa ctgaccgaag cgcttggccg cgatcagact cccatgctgg agcgcggcga
     1021 ggttcatgtc ggcatcagac acgatccggg cgatccacgt ttcgagagcc tcgtattgcc
     1081 agcggatgag gttctggccg cctgcactcc atcgctcgag cttggccacg ccggcatgat
     1141 cgatatcggc cgcctggcat cctatccgtt gctgctattg gaatcgggtt actcgattcg
     1201 gaggttgttc aatgcggctt gccgtctcgc agatgtcgag ccaaatatcg tgctcgagag
     1261 ccgcgcgccg cataccttgc tcgcgctcgc cgaggccggc cagggggtag cgatcattcc
     1321 gtcggtcctg cgaacggatc gccatacgtt gagaatcgtc cgcgtaaccc atcggcgcaa
     1381 accgcttcga gagcgatacg ccattcagtg ggacaagcgg cgtcccatgc cgccctatgc
     1441 cgaaagcttt tgcgaggcgc tcgccaaaca catgcggcac gtgcttccgg ttacgcggcc
     1501 atcgatcgag cagtccactg ccgcacgcaa gcaacgatga cgtccttgca cacgcacggt
     1561 atgcacgatg agctcgccga cggcatgagt cgcttccggt tcccccactg gagacgcgct
     1621 ggctcgtcac tggcttcacg aacggtccgc ttcgctcaaa acattcgcgt cattttgtgc
     1681 caacgtatat acttccgctc cagccgaacg gactcttcac cgccgatgaa ggaggcctcc
     1741 atgccagcgc aacctcgcaa ggtcgtgcat cccgacatca agcccgagaa cacgccggag
     1801 ctggcctcgc gctacctcga cgtccccaac atgccgtggg aaccgaccaa gttcgcgggg
     1861 attcggatca aggtgctcta tagcgacgac agcggcatca ccacggcgct gttcaagctc
     1921 gatccgggcg cgatcgtgcc gctgcacgag cacaccgcgc tggagcagac gttcgtgctc
     1981 gagggcaccc tcgaggatca cgagggcgcg tgcggaccgg gtcagttcgt ctggcggcca
     2041 gctggcaacc ggcatgaggc ggtggcgccc aacggcgccg tcatcctcgg cttcttcctc
     2101 aagccaaatc gctttgcccg cggcgagagg ttcttcaccg aggacgaggc gcagtagaag
     2161 cggccgcggc gaacgcgacc gaggctagcc gtgggcataa agttcgcatc aatccggcgc
     2221 cgtttgcgtc acctttgacg tgcgataaat tggaccgaat cggcagccct gaggcctgcg
     2281 aggacaccat ggcgcgcgag acctcgagag cagtcttggg ctgcgtctta attctgcttc
     2341 tcgccgccgt ggccggctgc tccgacttcg atttctgggg gaaaccggaa aaagccaacg
     2401 atcccaacgc ttatccggaa aactacaaaa aggacgtgct gacatacgtg aaaacccacc
     2461 cccgcgagct gctgaacgcg cgcgaagctt cgatctcgac gcccgcgctg aagcaattcg
     2521 gaacgcaaag ccggtatttc gcctgcctgc gagtcaacgg tcccgattgg cgcaaggaga
     2581 agatgctggt cttctactcc ggcgagatca atcaattcat cgacgcggag ggcgaccaat
     2641 gcggcgcggt cgcgtatcaa ccgttccccg aactggtgac ggagatcagc gagtttaagg
     2701 ggaagaaatg acgggaaatc ctttttccct tcgcccacga gagcatcccg gtaacgtacg
     2761 cttcgcgact catcgccgcg aacttgcacg ctaagttgcc gcgtgcaaat gtcgccgccg
     2821 ccacaaccct gacctcgcga aatcatgcgc tttggcacat gctttgcc
//
LOCUS       DATLJT010001517        11287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2439139,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001517 DATLJT010000000
VERSION     DATLJT010001517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439139"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VM910_34720"
     CDS             <1..211
                     /locus_tag="VM910_34720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI67699.1"
                     /translation="IDRARPRTHLSFGFGIHRCIGMRLAELQLKIIWEEIFKRFDRIE
                     VVEEPKRVYSSFIKGYETLPVRIAA"
     gene            251..1525
                     /locus_tag="VM910_34725"
     CDS             251..1525
                     /locus_tag="VM910_34725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI67700.1"
                     /translation="MNVQTAIRSEKAERLRLAREEAYATPLKDFHPGAPKLFQDDTLW
                     PWFERLRKEEPVHYCTNAPIEPYWSVVKYNDIMHVDTNHGTFSSDAALGGISIKDAPP
                     GYDWPSFIAMDQPKHAAQRKTVSPMFTPGHLDELAVLIRQRSAKVLDSLPRNETFNFV
                     ERVSIELTTQMLATLFDFPFEERRKLTRWSDVSTALPKSGIVESPEQRRQEMDECREY
                     FARLWNERVNAEPRNDLLSMMAHSDATRHMDPDNLMGNIILLIVGGNDTTRNTMSASV
                     LALNENPDEYRKLHENHKLIDSMVPEVIRWQTPLAHMRRTALIDTEIAGKTIKKGDRV
                     VMWYVSGNRDEEVIERPNEFIIDRARPRIHLSFGFGIHRCVGMRLAELQLRIVWEEIL
                     KRFERIEVVGEPRRLYSSFVRGIEYLPVRIPS"
     gene            1522..1959
                     /locus_tag="VM910_34730"
     CDS             1522..1959
                     /locus_tag="VM910_34730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI67701.1"
                     /translation="MSAQLNSPGLAETVNAKGFTRAAGFRIVKVEPGYAELAVTRRDD
                     LVQFFGHFHGGVITALADQAAGVAVTSGLPKGKIGVTVELKVNFLTPADRAELVARAK
                     TLQMSGSIGVATVELFARDEKSERLCAFCTATMRALDLPAEYR"
     gene            1956..3125
                     /locus_tag="VM910_34735"
     CDS             1956..3125
                     /locus_tag="VM910_34735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI67702.1"
                     /translation="MTAQDQHDELSDIRDAVAKLCAQFPGEYWRKLDREMAYPSAFVD
                     ALTQAGYLSVLIPEEYGGAGLKLSAATAILEEIHRAGCNAGACHAQMYTMGTVLRHGS
                     AEQKAQWLPQIASGKLRLQAFGVTEPTSGTDTSSLKTVAKREGNASYIVNGQKIWTSR
                     AEYSDLMILLARTTPKEQVRKRTDGLSVFLVDMREARKAGLSIRPIRTMMNHSTTEVF
                     FTDMRVPAENLIGDEGKGFRYILSGMNAERILIAAECIGDSKWFIAKATNYAKERAVF
                     GRPIGQNQGIQFPIAKAYASMRAAELMVKEATRKYEAGLDCGAEANMAKMLAADASWE
                     AANACIQTHGGFGFAEEYDVERKFRETRLYQVAPISTNLVLSFIAEHVLGMPRSY"
     gene            3135..4262
                     /locus_tag="VM910_34740"
     CDS             3135..4262
                     /locus_tag="VM910_34740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVI67703.1"
                     /translation="MLPLEGITVIAVEQAVAAPFCSSRLADAGALVIKVERPEGDFAR
                     GYDAAAKGQSSYFVWLNRGKESVVVDLATKDGRQQLEELIAGADVLLQNLKPGSMDKL
                     GFSRERLRRDYPALICCTITGYGDEGPYAHRKAYDLLIQAESGLASITGGPEGPSRVG
                     LSIVDIATGATAHASILEALFARSRTGQGADIRISMFDVMADWMAVPLINSESGNPPQ
                     RIGLAHPSIAPYGVFRSKDGKDILISIQSEREWKKLCAEVLGQPELPSDPRFSNMIER
                     VRNRTLTDKAVADMFATLSRNDLLQRLADADIAFAEVNTMADLAVHPHLRRIEVATPK
                     GVVAYPAPAAIFVGEPRRYGAVPAIGEHAERPKSSRVRTSS"
     gene            4259..5113
                     /locus_tag="VM910_34745"
     CDS             4259..5113
                     /locus_tag="VM910_34745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67704.1"
                     /translation="MTEKLDLDHLRQWIGRSTEASDVVTAQLVKGLRATLFQEIGEPK
                     NGDAAPFTVHWCLAQPVFPMSQLGPDGHPTRGGFLPPVPLPRRMWAGGELQFFDALRV
                     GDESTRKSRISDVTVKTGSTGSLCFVAVEHEVTTPRGLAVRERQDIVYREMSPAQAAG
                     PAKAASPPPAAKHRETHMADSVLLFRYSALTFNGHRIHYDRNYVTKVEGYPGLIFHGP
                     LQAAFIVEFAAKLRGGKPPKRLSYRGLQPLFEGSEFSVNANDNGGAMELWTANSDGQP
                     TMKGTATW"
     gene            5307..6926
                     /gene="mdlC"
                     /locus_tag="VM910_34750"
     CDS             5307..6926
                     /gene="mdlC"
                     /locus_tag="VM910_34750"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HVI67705.1"
                     /translation="MQKKSQTTKPLTVKQATFDLLRAFGIKKVFGNPGSTELPFLSDW
                     PDDIDYVLGLQEASVVGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQTPMV
                     ITAGQQARSILPLQAFLQAERASEFPRPYVKYSVEPARAEDVPPAIARAYYTAMQPPC
                     GPTFVSIPIDDWNHPTQAVHARKVSREIGPDKETMKALVTALAQSRNPALVVGPGVDR
                     AAAVDLMVKVAEKSKAAVWVSPFSARCSFPEAHPQFCGFLHASPGQLSDALRSHDLVV
                     VIGAPVFTFHVEGHAAIFDGAVPIFQITDDPTAAAVTRSGTSIIATMKPALTMLLELL
                     PESKRPAPAGRKIPPAPAAGDPIPVEFLLHSLFQAMPEDAALVEEVPSHRPAMQKFMP
                     MRGQDSFYTMTSGGLGYSLPAAVGMALGRGDRRTVCLIGDGSAMYSIQALWTAAQREV
                     PLTVVVINNAGYGAMRSFSQVMQVRNVPGLDLPGIDFVKIAEGHGCDAARVTKAAELQ
                     PALKRALAHKGASLVEVVVDSTVPLLYTQKN"
     gene            7069..7878
                     /locus_tag="VM910_34755"
     CDS             7069..7878
                     /locus_tag="VM910_34755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVI67706.1"
                     /translation="MTATDKLFAGSIPELYDRLLVPLIFEAYAQDLAERIAITRPQAV
                     LETAAGTGVLTSAMASRLPAQSRIVASDLNQPMLDRAATKRGLEGRITWRQADALALP
                     FEDRAFDVVACQFGVMFFPDKVKGYSEARRVLRPGGRFFFSAWDRISENEFGETVNDA
                     VAAFLPNDPPAFMARTPHGYHNVEKIREDLTAAGFTDISVTTIDHVSKAASPRDVAVG
                     FCQGTPLRNEIEARGASLEAATKAAAAALAKRFGNGPIEGRISAHIVAATA"
     gene            complement(7930..8724)
                     /locus_tag="VM910_34760"
     CDS             complement(7930..8724)
                     /locus_tag="VM910_34760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67707.1"
                     /translation="MQVAGKVVVVTGGANGIGKALCEAFHRAGAAKVVVVDVDGPAAR
                     AVAASINGAAFKCDVAKEKDVLHVIEETERQFGPIALFCSNAGVGGGFDPLSVNAGGD
                     SDEPFARSWAIHVMAHVYAARHLIPRYKVRGGGYFLNTISAAGLLSQVGSPAYSTTKH
                     AAVGFAENLAISHRADGIKVSILCPQGVDTNMLRAIPKGPQSGDGDLSPEQVARDALA
                     GVEQETFVILPHPQVLGYMRSKTENYDRWIAGMAKIQAKMREAHGK"
     gene            complement(8895..9590)
                     /locus_tag="VM910_34765"
     CDS             complement(8895..9590)
                     /locus_tag="VM910_34765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HVI67708.1"
                     /translation="MTIFKLKQLASTSIHAATGAIRWNYDRAELIADGVVHGIGILAG
                     VVAATVLVVLTAIYASARDILGVSIYVAGLLSMLVLSATYNLWPVSPTKWVLRRLDHS
                     AIYVLIAATYTPFIMELKDSVFAIALLVGVWCVAIFGIVLKLAWPGRFDRLSVGLYLA
                     LGWSGMMLYDSVIAALPQMALWFVLAGGALYSLGVIFHAWQRLRFQNVIWHCFVLLGA
                     ACHYTAVLNLVLA"
     gene            9769..11238
                     /locus_tag="VM910_34770"
     CDS             9769..11238
                     /locus_tag="VM910_34770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HVI67709.1"
                     /translation="MAPGLSDIIEEARLSARALLDFGEHLFNPTVRLGVTGLSRAGKT
                     VFITALVHGLTRGGRFPVFEAFASGRIARAYLAPQPDDAVPRFDYENHLRTLIEERRW
                     PDSTVDISELRLVIEYQRANGADRTLTLDIVDYPGEWLLDLPLLNKTYEQWARESLAL
                     SRETPRAHLAAEWHAHLATLAPEAAEDEQATRTAAKLFTDYLRACRDERFAMSLLPPG
                     RFLMPGSLAASPALTFAPLDVSVDGPAPPGSLWAMMARRYEAYKDAVVRPFFRDHFAR
                     LDRQIVLVDALAAFNSGPDALHDLEAALLGILDCFRVGRSTFLSSLLRPRIDRILFAA
                     TKADHLHHLSHDRLEAVLRRAVSGAVARAETTGAMIDVVALAAVRATREATVARGRDK
                     LPSITGTPVPGESAGDETFDGDTEVATFPGDLPADPEVLFDGESAFRGLSTAPADKSD
                     FRFLRFRPPRLVREGESEPVLPHIRLDRALQFLIGDRLQ"
BASE COUNT         2134 a         3736 c         3439 g         1978 t
ORIGIN      
        1 catcgaccgc gcgcggccgc ggacgcacct gtcgttcggc tttggcatcc accgctgcat
       61 cggcatgcgg ctcgccgagt tgcagctcaa gatcatctgg gaagaaatct tcaagcgctt
      121 cgacaggatc gaggtggtcg aggagccgaa gcgggtgtat tcgagtttca tcaagggcta
      181 cgagaccctg cccgtccgca tcgccgcttg atcgctcccc gcgcgttcgt tagtgggagg
      241 aaaaaccacc atgaatgtcc agaccgccat ccgctccgaa aaggccgaac gactgcgcct
      301 cgcgcgggaa gaagcctacg cgacgccgtt gaaggatttt catcccggcg caccaaagct
      361 cttccaggac gatacgctgt ggccgtggtt cgagcggctg cgcaaggaag aaccggtgca
      421 ttactgcacc aacgcgccga tcgagcccta ttggtcggtc gtcaaatata acgacatcat
      481 gcatgtcgat accaatcacg gaactttttc ttcggatgcc gcgctcggcg gcatctcgat
      541 caaggacgcg ccgcccggct atgactggcc gagtttcatc gcgatggacc agccgaagca
      601 cgcggcgcaa cgcaagaccg tgtcgccgat gttcacgccg ggacatctcg acgaactcgc
      661 cgtcctgatc cgccagcgct cggcgaaagt actggacagc ctgccgcgca acgaaacctt
      721 caatttcgtc gagagggtct cgatcgagct gacaacgcag atgctggcca cgctgttcga
      781 cttcccgttc gaggagcggc gcaagctgac gcgctggtcc gacgtctcga ctgcgctgcc
      841 caagagcgga atcgtggaat ccccggagca gcgccgtcag gagatggacg agtgtcgcga
      901 atatttcgca agactctgga acgagcgggt caatgccgag ccgcgcaacg atctgttgtc
      961 gatgatggcg catagcgatg ccacacggca catggacccg gacaatctga tgggcaacat
     1021 catcctgctt atcgtcggcg gcaacgacac cacgcgcaac accatgagcg cttccgtgct
     1081 ggcgctgaac gagaaccccg acgaataccg gaagcttcac gaaaatcaca agctgatcga
     1141 ttccatggtg ccggaggtaa tccgctggca gacacccctg gcgcacatgc ggcgcaccgc
     1201 gctgatcgat accgagatcg ccggcaagac gatcaagaag ggcgatcgcg tcgtcatgtg
     1261 gtacgtctcc ggcaatcgcg acgaggaggt gatcgagcga ccgaacgagt tcatcatcga
     1321 tcgtgcccgc ccgcgcatcc atctctcgtt cggcttcggc atccaccgct gcgtcggcat
     1381 gcgacttgcc gaattgcagc ttcggatcgt ctgggaggaa atcctgaagc gtttcgagcg
     1441 gatcgaggtg gtcggcgagc ccaggcggct ctattccagc ttcgtacgcg gcatcgaata
     1501 cctgccggtg cggatccctt cgtgagcgcg caactcaact cgccgggtct tgcggaaacc
     1561 gtcaacgcaa agggtttcac gcgcgcggcg ggctttcgca ttgtcaaggt cgagccgggc
     1621 tatgcggaac tcgcagtgac ccgccgcgac gatctcgtgc agttcttcgg ccatttccat
     1681 ggcggggtca tcaccgcgct ggccgatcag gccgccggcg tcgccgtcac ctcgggtctg
     1741 cccaagggga agatcggtgt caccgtggaa ctcaaggtca atttcctgac cccagccgac
     1801 cgcgcggagc ttgtggcgcg cgcaaaaacc ctgcaaatgt ccggctcgat cggcgtcgcc
     1861 accgtcgaac tctttgcccg ggacgaaaag tccgaacgcc tctgcgcatt ctgcaccgcc
     1921 acgatgcgcg cccttgatct gcccgccgag taccgatgac cgcacaagac caacacgacg
     1981 aactttccga catccgcgac gcggtggcaa agctctgcgc ccagttcccc ggcgaatatt
     2041 ggcgcaagct cgaccgcgag atggcctacc cctccgcctt tgtcgatgcg ctgacgcagg
     2101 ccggctatct ctcggtactg atcccggaag aatatggcgg cgccggcctg aagctgtcgg
     2161 ccgcgacggc gatcctggag gagatccatc gcgccggctg caacgccggt gcctgccacg
     2221 cccagatgta cactatgggg acggtactcc gacacggcag tgccgagcag aaggcgcagt
     2281 ggctgccgca gatcgcgagc ggcaagttgc ggctgcaggc gttcggcgtc accgagccga
     2341 cgtcgggcac cgatacctcc tcgctgaaaa ccgtcgcaaa gcgcgagggt aacgcgagct
     2401 atatcgtcaa tggccagaag atctggacca gtcgcgcaga atattccgat ctgatgatcc
     2461 tgctcgcgcg caccacgccg aaggagcagg tgaggaagcg caccgacggc ctgtctgtct
     2521 tcctcgtcga catgcgcgag gccagaaagg ccggactttc gatccgcccg atccgaacca
     2581 tgatgaatca ctcgacgacg gaagtattct tcaccgacat gagggtgccg gcggagaacc
     2641 tgattggcga cgaaggcaag ggctttcgct atatcctctc cggcatgaat gcggagcgca
     2701 tcctgattgc ggccgagtgc atcggcgatt caaaatggtt catcgccaag gccaccaact
     2761 acgccaagga gcgcgcggtg ttcggccgcc ccatcggcca gaatcagggg atccagtttc
     2821 cgatcgcaaa ggcctacgcc tcgatgcgcg cagccgaact gatggtgaag gaagcaaccc
     2881 gcaagtacga ggccggactc gattgcggcg ccgaagccaa catggccaag atgctcgcgg
     2941 ccgacgcctc ctgggaggct gcgaatgcct gcatccagac ccatggcggt ttcggctttg
     3001 ccgaggaata tgacgtcgag cgtaagttcc gcgagacgcg gctctaccag gtcgcaccga
     3061 tctcgaccaa tctggtgctg tcgttcatcg ccgagcatgt gctcggcatg cctcgctcct
     3121 actgagacaa acccatgctg ccgctcgaag gaattaccgt catcgccgtc gaacaggcgg
     3181 tcgcggcgcc cttttgctcg tcgcgcctgg cggatgccgg cgctctggtt atcaaggtcg
     3241 agcgtcccga gggcgatttt gcccgcggct atgacgccgc ggcgaagggg caaagcagct
     3301 atttcgtctg gctcaaccgc ggcaaggagt ccgtcgtggt cgacctcgcg accaaagacg
     3361 ggcgccaaca gctcgaagag ctgatcgcag gcgccgacgt gctgctgcaa aatttgaaac
     3421 cgggctcgat ggacaagctt ggcttctcgc gggagcgcct cagacgcgat tatcccgcgc
     3481 tgatctgctg caccattacc gggtatggcg acgagggccc ctacgcccat cgcaaggcct
     3541 atgacctcct gatccaggcc gagagcgggc ttgcctcgat taccggcggc ccggaggggc
     3601 cgtcgcgcgt gggcctctcg atcgtcgata tcgcgaccgg tgcgaccgcc catgcctcta
     3661 tcctggaagc gttgttcgcg cgctcgcgta ccggccaggg cgccgatatc cgcatctcga
     3721 tgttcgacgt gatggccgac tggatggccg tgccgcttat caattccgag tccggcaatc
     3781 cgccgcagcg catcggcctc gcccacccgt cgatcgcgcc ctatggcgtg ttccgctcga
     3841 aggacggcaa ggacatcctg atctcgatcc agagcgagcg tgaatggaaa aagctctgcg
     3901 cggaagtgct ggggcagcct gagctgccga gcgatccacg cttctccaac atgatcgagc
     3961 gcgtgcgcaa caggaccctc accgacaagg cagtggccga catgtttgcc acgctctccc
     4021 gcaatgacct gttgcagcgg cttgcggacg ccgacatcgc ctttgccgag gtgaacacga
     4081 tggccgacct tgcggtgcat ccgcatctcc gccgcatcga ggtggcaacc cccaagggcg
     4141 tcgtggctta tccggcaccg gccgcgatct tcgtcggcga accgcgccgc tatggtgcgg
     4201 tgcctgcaat cggcgaacat gccgaacgac cgaaatcgtc ccgcgtccgg acctcgtcat
     4261 gaccgaaaag ctcgacctcg accatttgcg acagtggatc ggacgctcga ccgaagcatc
     4321 cgacgtcgtc accgcgcaac tcgtcaaagg cctgcgcgcg acgctgttcc aggagatcgg
     4381 cgaaccgaag aacggcgatg ctgcgccctt cacggtgcat tggtgcctgg cgcagcccgt
     4441 atttccgatg tcgcagcttg gccccgacgg gcacccgacc cgcggcggct ttctgccgcc
     4501 ggtgccgctg ccgcggcgga tgtgggccgg cggcgagctg cagtttttcg acgcgctgcg
     4561 cgttggagat gaatcgacgc gcaaatcgcg catctcggat gtgaccgtga agaccggctc
     4621 gaccggcagc ttgtgcttcg tcgccgtcga gcacgaggtg acgacgccgc gcggcctcgc
     4681 cgtccgcgaa cggcaggaca tcgtctatcg cgagatgtcg cccgcgcagg cggcgggacc
     4741 ggcgaaggcc gcatcgccgc ctccggcggc aaagcatcgc gaaacccaca tggccgattc
     4801 ggtgctcttg ttccgctatt cggcgctgac cttcaacggc caccgcatcc attacgaccg
     4861 taactacgtc accaaggtcg aaggctatcc gggactgatc tttcacgggc cgctgcaggc
     4921 ggcgttcatt gtcgaattcg cggccaagct tcgcggcgga aagccgccga agagactctc
     4981 ctatcgcggc ctacagccct tgttcgaagg cagcgagttc tccgtcaacg ccaacgacaa
     5041 tggcggcgcc atggagctgt ggaccgcgaa ctctgacggc cagcccacga tgaagggcac
     5101 ggcgacgtgg tgaggtccgt tgcggccacg atatcggtcc actccctcgc cccggtcgcg
     5161 ggggagggtt ggggtgagga aagtttccac atggcgaacc tcgctgacag cccccctcac
     5221 ccggatcgca tctaacgatg cggtccgatc tctccccgca agcggggcga ggcgaagctt
     5281 gtgccgacag cgaaggacga ttgatcttgc aaaagaagtc gcaaacaacc aaacccctca
     5341 ccgtcaagca ggcgactttc gacctgttgc gcgccttcgg catcaagaag gtgttcggca
     5401 atcccggctc gaccgagctg ccgttcctct ccgactggcc cgacgacatc gactatgtgc
     5461 tgggcctgca ggaggcttcc gtggtcggca tggccgacgg ctacgcgcag gccacgcgca
     5521 atgccggttt cgtcaacctg cattcggcgg ccggtgtcgg caacgcgctc ggcaacatct
     5581 ataccgctca tcgcaaccag acgccgatgg tgatcaccgc ggggcaacag gcccgctcga
     5641 tcctgcccct gcaggctttc ctccaggccg agcgcgcatc cgaatttccg cggccttatg
     5701 tcaaatattc cgtcgagccg gcgcgtgccg aagatgtgcc ccccgcgatc gcacgcgcct
     5761 actacaccgc aatgcagccg ccctgcggcc caacgtttgt gtcgatcccg atcgacgact
     5821 ggaaccatcc gacgcaagcg gtgcacgcgc gcaaggtcag ccgcgagatc gggccagata
     5881 aggaaacgat gaaggcgctg gtcacggcgc tcgcccaaag caggaacccc gcactcgtcg
     5941 ttggccccgg cgtcgaccgc gccgcggcgg tcgatctgat ggtcaaggtt gcggagaaga
     6001 gcaaagccgc ggtctgggta agcccgttct cggcgcgctg ttcgtttccg gaagcccatc
     6061 cgcaattttg cggcttcctg cacgcctcgc cgggacagct ctcggatgcg ctgcgaagtc
     6121 acgacctcgt ggtcgtgatc ggcgcgcctg tcttcacctt ccatgtcgaa ggccatgccg
     6181 cgatcttcga cggcgcagtc ccgatcttcc agatcactga tgatccgacc gcggccgcgg
     6241 tgacgcgctc gggcaccagc atcatcgcta ccatgaagcc cgcgctcaca atgctgctcg
     6301 aactccttcc ggaatcgaag cgcccggcgc ctgcgggccg aaagatcccg cccgcgcccg
     6361 cagccggcga tccgattccg gtggaattcc tgctgcattc gcttttccag gcgatgcccg
     6421 aggatgccgc tctggtcgag gaagtgccct cgcaccggcc ggcaatgcag aaattcatgc
     6481 caatgcgcgg ccaggacagt ttttacacca tgacgagcgg cggcctcggc tacagtctgc
     6541 cggccgcggt cggcatggcg ctcggacgcg gtgatcgccg caccgtctgc ctgatcggcg
     6601 acggttcggc gatgtattcg atccaggccc tgtggacggc cgcgcagcgt gaggtgccgc
     6661 tcaccgtggt ggtcatcaac aatgcaggct atggcgcgat gcgctccttc agccaggtga
     6721 tgcaggtgcg caatgtgccc gggctcgatc tgcccggaat cgactttgtg aagatcgccg
     6781 agggtcacgg ctgcgatgcg gcgcgtgtga ccaaggcggc cgagcttcag cctgcgctga
     6841 aacgtgccct cgcgcacaag ggcgcgagcc tggtcgaggt ggtcgtggat tccaccgtgc
     6901 cgctgctcta cacgcagaaa aactagagga tgagccggaa aagtcgcggc ccgaccggcg
     6961 acgcgatgcg tcaaaaggtg acagtcacat cggatcaaaa tcacatttct cctgaagcat
     7021 ccgtttcata attctcgcgg ggaaagaacc acgcaccgga gctagtcgat gaccgcgacc
     7081 gacaaacttt tcgcaggatc catccccgaa ctctacgacc ggttgctggt tcccctcatc
     7141 ttcgaggcct atgcgcagga tctcgcggag cgaattgcca taacacgacc tcaggccgta
     7201 ctggagacgg ccgccggcac tggcgttctc acgtccgcca tggcatcgcg tctgccggca
     7261 caaagccgga ttgtggcaag cgatctcaat caacccatgc tggatcgcgc cgcaacgaag
     7321 cgcggtctcg aaggccgtat cacgtggcgg caggcggacg cactggcctt gccgtttgaa
     7381 gatcgcgctt tcgacgtcgt cgcctgccaa ttcggcgtca tgttctttcc ggacaaggtc
     7441 aaaggttaca gcgaggcccg ccgcgtgctc aggccgggcg gccgcttctt cttcagcgcc
     7501 tgggatcgta tctccgaaaa cgagttcggc gaaaccgtca atgacgcggt ggccgccttc
     7561 ctgccgaacg atccgccggc attcatggcg cgtaccccgc acggctatca caacgtcgaa
     7621 aagattcgcg aggacctgac ggccgccggc tttacggata tttctgtgac aaccatcgat
     7681 cacgtcagca aggctgcttc gccgagggat gtggccgtcg ggttctgtca gggcactccg
     7741 ctccgaaacg aaatcgaagc gcgcggcgca tcgcttgaag ccgcaaccaa agctgcagca
     7801 gccgcactgg cgaagagatt tggcaatggc ccgatagaag gccggatcag cgcgcacatc
     7861 gtcgcggcca ccgcctgagt aggcgtcatt cccggtagct cgcagagctg accctggtca
     7921 gcctgcgggt catttcccat gcgcttcccg cattttggcc tggatcttgg ccatgcccgc
     7981 gatccagcgg tcgtaattct cggtcttgct gcgcatataa ccgagcacct gcgggtgggg
     8041 caggatgacg aaggtctcct gctcgacgcc ggcaagcgcg tcccgagcca cctgctccgg
     8101 cgacagatcg ccgtccccgg attgcggacc tttcggaatc gcgcgcagca tgttggtatc
     8161 aacgccctgc gggcacagga tcgacacttt gatgccgtcg gccctgtggg agatcgcgag
     8221 attttccgca aatcccaccg ccgcatgctt ggtcgtcgaa taggccgggc ttccgacctg
     8281 cgacaacagg cccgccgcgg aaatcgtgtt gaggaagtag ccgccgccgc gcaccttgta
     8341 gcgcgggatc aggtgtcgcg cggcatagac atgcgccatc acatggatcg cccaactccg
     8401 cgcgaacggt tcgtcggagt cgccgccggc attcaccgaa agcgggtcga acccgccgcc
     8461 aacaccggcg ttggaacaaa acagcgcaat aggcccgaac tgccgctcgg tttcctcaat
     8521 gacgtgaagc acgtcctttt ccttggcgac atcgcatttg aaagcggcgc cattgatcga
     8581 ggctgcgacc gcccgcgcgg ccgggccgtc gacatcgacc accaccacct tggcggcgcc
     8641 cgcacgatga aacgcctcgc acagcgcctt gccgatgccg ttggcaccgc cggtgacaac
     8701 cacgaccttg ccggccacct gcatcgtgac ctcccgctgt tatttgttga ctgatgtcct
     8761 caacccgaaa cgcgcaagac ttcgcagcaa gctctttctg cgacttcggt tcaaagggac
     8821 actagcaaat ttacgatcct ggtgtggttt tggttcggaa gttcttttga cgaaccctca
     8881 gccccaccac ggccctaggc cagaaccaga ttgagcacgg cggtatagtg gcacgccgct
     8941 cccagcaaca cgaaacaatg ccagatgacg ttctggaagc gcaaccgctg ccaggcgtga
     9001 aaaatcacgc ccaggctata gagcgcgccg cccgccagca cgaaccacag cgccatttgc
     9061 ggcaatgcgg caatgaccga atcgtaaagc atcatgccgc tccagccgag cgccagataa
     9121 agcccgaccg aaagacggtc gaaacggccg ggccaggcga gcttgagaac gatgccgaag
     9181 atcgcgacgc accacacgcc gaccagaagc gcgatggcga agacgctgtc cttcaattcc
     9241 atgatgaacg gcgtgtaggt cgcggcgatc agcacataga tcgccgaatg gtcgagccgc
     9301 cgcagcaccc atttggtggg cgaaaccggc cagagattgt aggttgccga cagcaccagc
     9361 atcgacagca ggcccgcgac atagatcgag acgccgagga tatcgcgcgc gctggcatag
     9421 atcgcggtga gcaccaccag cacagtcgcg gcgacgaccc ccgccagtat cccgatgccg
     9481 tgcaccaccc cgtccgcgat cagctccgcg cggtcgtagt tccagcggat tgcgccggtt
     9541 gccgcatgga tggaggtcga agccaactgc ttgagtttga agatcgtcat gggccacccg
     9601 cagggctatc ggaatgatag aataacatag gcgccaggag gtcaatgaag cattgaaaat
     9661 ccgtgtcgcc tgcaatcgcg acgaaatgtg aagcttgatt tcatccctac aattgccact
     9721 ttcggattgt tcccgcccct ctcgccacct gcccagcttg ctgcccccat ggcccccggt
     9781 ctatccgaca tcatcgaaga ggcgcgcctg tcggcgcggg cgctccttga ctttggcgag
     9841 cacctcttca acccgacggt ccggctcggc gtcaccggcc tgtcgcgggc gggcaagacc
     9901 gtattcatca ccgccctggt ccacggcctt acgcgcggcg gccgcttccc ggtgttcgaa
     9961 gccttcgcgt cgggccgcat cgcgcgcgca tatctcgcac cgcagccgga tgatgcggtg
    10021 ccgcgcttcg actatgagaa ccacttgcgc actttgatcg aggagcggcg ctggccggac
    10081 tcgaccgtcg atatcagcga gctgcgcctt gtcatcgaat accagcgcgc caatggcgcc
    10141 gaccgcacgc tgacgctcga tatcgtcgac tatcccggcg agtggttgct cgacctgcct
    10201 ttgctcaaca agacttacga gcagtgggca cgcgagagcc ttgcgctctc gcgcgagacg
    10261 ccgcgcgccc atcttgcggc ggaatggcac gcccatctcg caacgctggc gccggaagca
    10321 gccgaggacg agcaggccac ccgcacggcg gcaaagctct tcaccgacta tttgcgggcg
    10381 tgccgcgacg agcgtttcgc catgagcctg ttgccgcccg gccgcttcct catgcccggc
    10441 agtctcgctg cctcgccggc gctgaccttc gcgccgctcg atgtatccgt tgacgggccg
    10501 gcgccgccgg gctcgctatg ggcgatgatg gcgcggcgct acgaagccta caaggacgcg
    10561 gtagtccgcc cattcttccg cgatcatttt gcgcgcctcg accgccagat cgtgctggtc
    10621 gacgcgctcg cggcgttcaa ttcgggacct gacgcgctgc acgatctcga ggcggcgctc
    10681 ttggggattc tcgactgttt ccgggtcggc cgcagcacct ttctgtccag cctgttgcgg
    10741 ccgcgcatcg accgcatcct gttcgcggcc accaaggccg accatttgca ccatctcagc
    10801 cacgaccgac tggaagccgt gctgcgccgt gccgtctcgg gcgccgtggc gcgcgcggaa
    10861 acgacgggtg ccatgatcga cgtggtggcg ctcgccgcgg tgcgcgcgac gcgggaagcc
    10921 accgtcgcgc gcggccgcga caaattgccc tcgatcacag gcacacccgt ccctggcgag
    10981 agtgccggcg acgagacatt tgacggcgat acggaagtag cgacctttcc cggcgatctt
    11041 cccgccgatc cggaggtgct ctttgacggt gaaagcgcgt tccgcggtct ttccacggcg
    11101 cctgcggaca agagcgattt ccgcttcctg cgcttccggc cgccacggct tgtgcgcgag
    11161 ggcgagagcg agcccgtgct gcctcacatc cgccttgacc gggcgctgca gttcctgatc
    11221 ggagaccgat tgcaatgaac gaacgaggaa agcaccggcg gccggcgacc ttccggctcg
    11281 acgattc
//
LOCUS       DATLJT010001518         2683 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3589476,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001518 DATLJT010000000
VERSION     DATLJT010001518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3589476"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..678)
                     /locus_tag="VM910_34775"
     CDS             complement(271..678)
                     /locus_tag="VM910_34775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67710.1"
                     /translation="MRRDRAILRGLGVARWDGAKPATPFGADFDRMAPFRIGATVCGG
                     ASRLVRLVSGCELQGIADALVIRSALSIEANSDPADREWSGSASATIKALQQSGMTIG
                     VATLGTIVSTGAVLSLTHAWSGANFPRAAAIGR"
     gene            complement(1075..>2683)
                     /locus_tag="VM910_34780"
     CDS             complement(1075..>2683)
                     /locus_tag="VM910_34780"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67711.1"
                     /translation="QPHEPTPGSPLSRLLGGERIVQITDMAELAKQHPDDLRAQAGAE
                     YGLRTVLFVPLRKDGALLGHITAGRLDEVRPFSDKQIALLQNFAAQAVIAMENARLLT
                     ETREALDQQTATAEVLGVINSSPSDLAPVFDAMLDKATRLCEADFGVLLTYAEDHYRA
                     AAFHNVPSAYVSALQEPIRPSAKMALGRIARGDLVAQIEDAAADEELLSHPTVRGGVE
                     LAGFRTVLAVALRKDGALLGAITIHRQEARLFSDKQSALLQNFAAQAVIAMENARLLG
                     ELRQRTDEVAELNRGLEARVAEQVDELGRVGRLKRFLAPQLAELIVSQGDEKILESHR
                     RDIVVVFCDLRGYTAFTETAEPEEVLNFLREYHGALGPLVSQFEGTLDQFSGDGIMVF
                     FNDPVPCPDPAERAVKMAMAMREGAGKLIAAWRRHGSELGFGAGIAQGYATLGQIGFS
                     ERSGYTAIGTVCNLAARLCAEAKDGQILVSSRIAEAVEAVATLEDLGNLELKGLRRPV
                     AAFNVVQSTSPAEARPNLTVVARGPEA"
BASE COUNT          477 a          903 c          808 g          495 t
ORIGIN      
        1 aggccgcctc agatggacta acggcgtgaa gtgcctcaac gtcgaacgac cgaacgcggt
       61 gcttctcgcc cgttgccccg cgttcccgcg aaggtccgtt ggctgaaccg acaacggccg
      121 ctcagccttg gcggcgggaa ctggtgctca tgccccaagg cgacattcgt ccgcgttccc
      181 gcctgaagca tggccgtgtt tcccgcacgc tcatcagccc ctggcaattt ccctttcact
      241 cgctgcgccg tacgttcctg tcgagttgtt ttatctacct atcgccgccg ctcggggaaa
      301 attggcgccg gaccaggcat gggttaggga taagacggcg ccggtactca cgatcgtgcc
      361 gagtgtggcg acgccaatcg tcatgccgct ttgttgcagt gctttgatgg tcgccgaagc
      421 cgacccactc cactcccgat cggccggatc tgaattggcc tcgatcgaca gtgcactgcg
      481 gatcaccagc gcgtcggcaa tgccttgtag ctcacaaccg gaaacaagcc ggacgagccg
      541 cgacgcgccc ccgcaaaccg tcgcaccgat cctgaaaggg gccatgcggt cgaagtcagc
      601 gccgaaaggc gtcgccggct tggcgccatc ccatcgcgct acgccgagac cccgtaggat
      661 ggctctgtcg cgccgcacgc cgcggcgcgg cgcactcacc atccggtacc tgtcgccctc
      721 ggataaagtc caggcttcgc ctggagttct ccgcgctact ctgccgaggc cggtcggcgc
      781 ttgcgccgcg ccaccccaag ggatagcttt ccacgtaaac gccgcggcgc aaaaaacgcg
      841 gccccgatgc aggcagggag cccggagcta tgggtgcagt acgttttccg ccgccaccga
      901 ctctacgcaa ccccgtcgtt gcggcgcgtt ccggcggtcg tctcctttct gaatcctcag
      961 ccgacactca gccttggcgg cgggaaccgc tcttcatgcc ccttaagaga ccttcgcggt
     1021 cggctcggga attagctcag ttcggtggtc cttgaggcct cgcgttgtct aagatcaggc
     1081 ctccggtccc ctcgcgacga cggtcaagtt gggacgagcc tccgccggac tggtgctctg
     1141 cacgacgttg aatgccgcaa ccggacggcg aagacccttt agctccagat ttccgagatc
     1201 ctccaacgtc gcaaccgcct cgaccgcttc cgcgatgcgg ctgctgacca ggatctgccc
     1261 gtccttagcc tcggcacaca gccgggcggc gagattgcat accgtgccaa tcgcggtgta
     1321 gccggagcgt tcggaaaagc cgatctgacc cagggtcgcg tagccctgcg cgatacccgc
     1381 gccaaaacca agttcgctgc cgtggcgacg ccaagcggca atcagcttgc ccgccccttc
     1441 acgcatcgcc atcgccatct tgaccgcgcg ttccgccggg tcggggcatg gcaccggatc
     1501 gttgaagaac accatgatgc cgtcgcccga gaactggtcg agcgtgccct cgaactggga
     1561 gacgagcggc cccaaagcac catggtattc gcgcaagaaa ttgaggactt cctcgggctc
     1621 ggcggtctcg gtaaacgcgg tgtagccgcg tagatcgcag aacacgacga caatatcgcg
     1681 ccgatgactt tcgaggatct tctcgtcgcc ctgtgagacg atcagctcag caagctgagg
     1741 tgccagaaac cgcttcaacc ttcccacccg gcccaactcg tcgacctgct cggcgacgcg
     1801 tgcctcaagc ccacggttta attctgccac ctcgtcggtg cgctggcgca attcgcccaa
     1861 gagccgcgcg ttctccatcg cgatgaccgc ctgggccgcg aagttctgca acagcgcgct
     1921 ctgcttatcg gaaaacaggc gcgcctcttg gcggtggatc gtgatcgcgc ccagtagcgc
     1981 tccgtccttg cgtagcgcga cggcgaggac ggtacggaag cccgcaagct ctaccccccc
     2041 acgcactgtt ggatgtgata acaactcttc gtctgctgcg gcgtcctcga tttgagcgac
     2101 cagatcgccg cgggcaatgc ggccaagcgc catctttgca ctgggccgta tgggttcctg
     2161 caaggcagac acgtaagccg acggtacatt gtggaaggcg gcagcccgat agtggtcctc
     2221 tgcataggtc aacaaaactc cgaagtcggc ctcgcacagc cgtgtcgcct tatccagcat
     2281 cgcatcgaac accggggcta ggtcgctggg cgaagagttg atgactccca agacctccgc
     2341 ggtcgcggtc tgctgatcca aggcctcgcg cgtctcggtc aacagccgcg cattctccat
     2401 ggcgatgacc gcctgcgcgg cgaagttctg cagtagcgcg atttgcttgt cagagaaggg
     2461 ccgcacctcg tcgaggcgac cggctgttat gtggccgagc agagcgccgt ccttgcgcag
     2521 tggcacgaag agcacggtgc ggagaccgta ttcggcaccc gcttgggctc tcagatcgtc
     2581 ggggtgttgc ttagccaatt cggccatatc ggtgatttgg acgatccgtt cgccgccgag
     2641 caggcgcgaa agaggcgatc ccggagtcgg ctcgtgcggc tgg
//
LOCUS       DATLJT010001519        10579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4048919,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001519 DATLJT010000000
VERSION     DATLJT010001519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4048919"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1206)
                     /locus_tag="VM910_34785"
     CDS             complement(178..1206)
                     /locus_tag="VM910_34785"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVI67712.1"
                     /translation="MPNLRRIALVGEPFERQPFRRHFSQELASLAAEVEFIDLLGLPT
                     AELRTRVATLPDDSAIYYTAIYSITGIPRDAVPMLAEVANRPIVSDTVTHIGYGSTGG
                     IVVLPEPIAQETARRALRILDGEPAARIRPAGGDFTKPVFDWRQLQRWKVSERALPPG
                     SEIRFRQPGLWEQYHWVVIAALTVVLIQAAMIFWLLFERRRRRLAELDSRRRIFEVAH
                     LNRAAAAGALSASIAHELNQPLGAILSNAEAGELLLRANPLDVGQLKEILADIRQADQ
                     RAADIITHLRQLLKKRSEIDLQEFNVNDAIADAIHVLEPEAMKRQVALSVDPAPRPLP
                     VRADPVHL"
     gene            2171..4630
                     /locus_tag="VM910_34790"
     CDS             2171..4630
                     /locus_tag="VM910_34790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVI67713.1"
                     /translation="MKFTEKLSLGTLTLLAAVTACSIGHAQQVTGTPGSPSATTTIEG
                     NQLPAPPRKFGGVIEESAKDSRPWWPPRVVPPKGAPNILLIMTDDQGYGVSGTFGGVI
                     PTPASDRIAKAGLRYTEFNSTALCSPTRAALITGRNHHSVGFGVITELSTGYPGYDSI
                     IGPENATLGTILRDNGYATSWFGKNHNTPAFQYSLAGPFDQWPSGMGFNYFYGFMGGE
                     TDQWTPYLFRDHSQIFPWVGKSDYNLITDMADEAIKYMRELNANAPDQPFFLYYVPGG
                     SHSPHNPKKEWVEKFKGKFDMGWEKLREQIFANQKRLGVIPANAQLTPWPDDLPKWET
                     LSADEKKLFARQAEVFAGYTAYTDNEIGRVIQEVENLGKLDNTLIIYIVGDNGTSPEG
                     SKLGTPNQYTAYNGILDVPIDAQLQFYDVWGSSATYPHMAVAWSWAFDTPFKWTKQVA
                     SHFGGTRQGLAISWPARIKDVGGIRHQFHHIIDIVPTILEVTGIKAPEYVNGIKQKPI
                     EGVSMAYTFDKANANAPSTHKTQYFEMASNRGIYHDGWYANTTPPVPPWVLNAPMPPV
                     NEYKWELYNLTEDYTQYNDLAAKMPDKLKEMQALFVQEASKYNVFPLQNSTFARAIDP
                     RPSDTAGQTVFTYSGMMPGIPSGNAPSILNRSFTITAEVDVPQGGGNGMIVTEGGRWG
                     GFGLYLLNGKPVFSYNLLMLLYPRWTGNEPLTAGKHTIVFDFKYDGPGIAKGGTGVLK
                     VDGREVHTLSIPKTIPFLLPADETFDVGVDTRTGVNDLDYKVPFQFNGTINKVTFNLG
                     PVQLTSDEHQAIRHARAKAND"
     gene            4734..5018
                     /locus_tag="VM910_34795"
     CDS             4734..5018
                     /locus_tag="VM910_34795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67714.1"
                     /translation="MTKSIGAAVCAMALISAGGVAAQGMLLEFAADKVIKKYESATCE
                     QLKVQKNEPRTEREKMAIDFLRHDSQARVAFIDKIAAPVLNKLFECDLIP"
     gene            5079..5927
                     /locus_tag="VM910_34800"
     CDS             5079..5927
                     /locus_tag="VM910_34800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67715.1"
                     /translation="MSQSHFTLRFPLKSSADAKAVAQELPPLMPDLFRAEDAIGTVHY
                     SRFTVLSDRTLLFLGDFDGDFGPLMRELGRSTGQVFDKIFKHVSDPPPTPVASNPDAF
                     TEWAAEHLLSPAYVFSAYPGPTVKEIKAMAAAAGVEGRTQLLPFLVILPARGRVAFAE
                     MQLILRTMAMTGKVDRDMTSVGTPHFAQFVPLEDQQLGFFTVYDGAFDKYIMDFTRNI
                     GEVFDVIFKFTKDPPPSPCRKHVQEFIDFAAGASRAPIGFYQAYPGLSNQDIKALIAD
                     SGTKAA"
     gene            5955..7514
                     /locus_tag="VM910_34805"
     CDS             5955..7514
                     /locus_tag="VM910_34805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67716.1"
                     /translation="MSQDSNTRSPAGERREGLFHHLFERIEGRVHSTAAAKTALDLED
                     IQGLILRGYRMPMVRHFLLKVTAPAAARTLIGRLVSGDEADAPQITTAKDWHVGFEPG
                     PGDDPASVPRCKPDYCLNVGITWPGLLALEVKDRIPNLSFKSFKAFMAGAAARAELVG
                     DTGANGPQHWIGGFGTGNDHVLVTLHAISPEAMRSYSDRLSELLSQGAAFEEIWRGDG
                     MALMEMQDGKPVPTTKVHFGYTDGISMTTIRGGPEHYTRDHQRPCEPWLFVLLDEAEN
                     YVLPGPKQLSRNGSFAVFKMIRTDVVGFENFLQSHKDRIDPELLAAKLCGRWRNGVPL
                     MLSPDTDKPAGGLAPDALNDFEYVNADGSGDPKGLRCPVGAHMRRINPRGQPVIGQGL
                     PGGSNNTHRLIRRGMPYGPTYDPTKPYDGMERGLLGYFINSSIENQYEFVLSQWVNDA
                     EFAGSVRLPPKSRDPIIGTQNPEESIFVIPQANGAEPIKITGFSSFITTRAAAYCFLP
                     SISAMKLIAGL"
     gene            7565..8680
                     /locus_tag="VM910_34810"
     CDS             7565..8680
                     /locus_tag="VM910_34810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67717.1"
                     /translation="MSFLTRLLGLTVSDLERSLATEPAVIAEISEKIILMQKNAAAEQ
                     RRPICRGTHAKGVCARAQFEVFDVSVGRDRALTARLAKGIYAKPGIYPAVVRFANADP
                     KVNSDFKSDVRSLSFSVELAPGGAAAWGAKISCQDYSMQNATTLPLNDAHAFLVAMEV
                     LTASSPAKAIHSLPWRDKLIFARTMILAQLQAHQTVKPYQQLRYWSTVPFRHGPADVV
                     KYSATPSVDNPARPLQKKYPNALQDEFARHLNEDAKMSSFDFALQFLDTERMTYWGKR
                     RDAGFWIENASVEWKETQAPFHRVARLSLVPKSKLEQDVSESLYFDVTGNSLPDSTPL
                     GSINRARWPAELASRKARQTHVQSAMKRLAHQSGNLP"
     gene            8677..9729
                     /locus_tag="VM910_34815"
     CDS             8677..9729
                     /locus_tag="VM910_34815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HVI67718.1"
                     /translation="MTPRSPLICCVGAASLMFGTFCLVQKGAAHAELGSCGPYSGLPS
                     DDTDTAGMVFIRGGTFVMGSERHRREERFTHVVRVDGFWIDRHEVTNAQFREFVTATG
                     YVTLAERGLDPATRPEMSKELLAPGSVVFIPPANVASGGRITQWWQYVAGANWREPTG
                     PGSSIAGKDNHPVVHIAYEDALAYARWRGRELPTEAQWEFAARGGRDGEDDWSSAFDA
                     EGKPIANTWQGVFPVYNSNEDGYVGTAPVGCFKPNGYGLYDMIGNVWEWTSDWYRPGH
                     PREAAVNPSGPELQDIPLARGQSPSRVIKGGSYLCASNYCSRYRPAARQPQEIDLGAA
                     HLGFRTVSNRSDSETR"
     gene            complement(9877..>10579)
                     /locus_tag="VM910_34820"
     CDS             complement(9877..>10579)
                     /locus_tag="VM910_34820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVI67719.1"
                     /translation="IGIYGRADTFLAGSVTGALTTPLGTLPFSRFDSISDSVSGFGDV
                     FPLATLRWNNGVHNYMTYVMGDVPVGAYDSTRLSNIGIGHSGIDAGAGYTYLNPQTGH
                     EFSGVLGFTYNFTNQSTQYQNGVDMHFDWGASQFLTKQVQVGVVGYVYRQLGCDTGAG
                     DRVGCFQSQVVGAGPQLGFIIPLTTETQGYLNLKSYWEFANQNRPDGWNAWVTFVISP
                     AEQTPSAPPRRMITK"
BASE COUNT         2141 a         3282 c         3270 g         1886 t
ORIGIN      
        1 gaaagtttcg atgacctttt tcaatttgtc cctctgaagg ccgggttccg agtcgcaaat
       61 cgagacctcg acctcggatc tctcgttcaa ggcggtttgc agggccaatt gacgctcagc
      121 cggcggagaa gtggccatag catccattcc gttcatcgcc agattcaata tcacctgcta
      181 caggtgtacc gggtctgcgc gcacgggcaa cggccttggt gccggatcga cgctaagcgc
      241 cacctgcctc ttcatcgcct cgggctcaag gacgtggatc gcgtccgcaa ttgcgtcatt
      301 gacgttgaac tcctgcaaat cgatctcgct tcttttcttc aataattgac gcaggtgcgt
      361 gattatgtct gccgcgcgtt ggtccgcttg gcggatatcg gcgagaattt ccttgagctg
      421 cccgacgtca agtggatttg ccctgagcag gagttcgcct gcctcggcgt tgctcaggat
      481 ggcgcccagg ggttggttga gctcatgggc gatcgaggcc gagagcgccc ccgcggcggc
      541 ggcccggttc aaatgagcga cttcgaagat gcggcgccgg gagtccagtt ccgcgaggcg
      601 acggcggcgg cgctcgaata gcaaccaaaa aatcatcgcc gcttgaatca ggacaacggt
      661 gagcgccgct atcaccaccc agtgatattg ctcccacaag ccgggctgcc ggaaacgtat
      721 ttcgctgccc ggtggcagcg cgcgttcgct gaccttccag cgctgcagtt gacgccagtc
      781 gaagacgggc ttggtgaaat ctccgccggc agggcggatc cgcgcggcgg gctcgccgtc
      841 gagaatgcgc agggcacgcc gcgcagtctc ctgtgcgatc ggttcaggga gaacgacgat
      901 tccgccggtg ctgccatatc cgatatgggt caccgtatcg ctcacaatcg gtcggttagc
      961 cacttccgcc agcatcggaa ccgcatcacg cggaatgccc gtgatgctgt agatggccgt
     1021 gtagtaaatg gccgaatcgt ccggcagcgt agccacgcgt gttctgagtt ccgccgtcgg
     1081 caagccgaga agatcgatga attcgacctc ggcggcgagt gacgcgagct cctgggagaa
     1141 atggcgccgg aaaggctgac gctcgaatgg ttcgcctacc agggcaattc gcctgaggtt
     1201 cggcacgagt gcccgcgccg cggtaaccgc gttgcgcatg gtcaatcgaa aggtcgttcc
     1261 ggtgacgtcg ggcggaaggc gcgggagatt ctgtgagtcg acggcaccga agacgaccgg
     1321 caccgatgac caaagctccg cgcggaagcg caaaaggatt tcgagtgccg ttgcgccgtg
     1381 ggcgatgatg acgccgattg gcttgccctg atacttctcc cgcaaataag tccgtagaag
     1441 ctctgcgtat tgcgggctgt tgaatcgacc gagatcaagg atttccgaat atatgaccag
     1501 agaggtcggc gaacctgctt tcagcgcgga cacaagcgct gccctgaact ccaagcccca
     1561 cggcgtgttt gagtcggatt gatcgaagat cagcaccgag cgcggcaatg gctcggcagc
     1621 agccgggatc tccgggacca ttgctgccag cagaacgaat gctcgcagta acagcttcaa
     1681 ataatgcacg agttggcgtc ccaactgcgc aagtgcggat tctctccctt accgaaatac
     1741 cattccgcgg tagggagcca tcaaaatcaa gatacagcat gcaaggtttt gcaagctcaa
     1801 atcattgaca tggatcatgg cgctcgcatt cgcagggcgc tgattgtcat aaaagttcca
     1861 cataaaactc tggtcgctcg cgctttggcg tcagcgtgcg gtcgtcttgc aactgcgctt
     1921 acgtcgtccc gaggccacgt gcctgcaagc cgttcaagaa gtcagcgagt caccataccg
     1981 cgcgatcgca caaacgcagc gtgcgatatc caagctgcac ctaaggacaa tatcgctcaa
     2041 tcgatgaggg cgccaaactt acaatccggc tggacgtgaa cccgacggat cagagctgcc
     2101 attgcgatcc gctacgtcca cgtgatgacc ggcgtccgcg gcggcgccca ctgctcagtg
     2161 gagggatgcg atgaaattca cggaaaagct ttcgcttggt acgctcacat tgctggctgc
     2221 cgtgacggcg tgcagcatag gccatgcgca gcaagtcacc ggcacgcccg ggtcgcccag
     2281 tgcgacaacc accatagagg gcaaccagct cccggcaccc cctcggaaat tcggcggagt
     2341 gatcgaggaa agcgctaagg actctaggcc ctggtggccg ccgcgcgtgg tgccgcccaa
     2401 gggcgcgccc aatattctgc tgatcatgac tgacgaccag ggctatgggg tcagcggcac
     2461 gttcggcggc gtgatcccga cgccggcgtc ggatcggatc gccaaggcgg ggctgcgcta
     2521 caccgagttc aactcgaccg cgctgtgctc acccacgcgg gcggcgctga tcaccggccg
     2581 caaccaccac tcggtgggct tcggcgtgat cacggagctc tccacgggct acccgggcta
     2641 tgactcgatc atcggtccgg agaacgcgac cctcggcacg atcctgcgag acaacggcta
     2701 tgcgacatcg tggttcggca agaaccacaa cacgccggcc ttccagtaca gcctggcggg
     2761 ccccttcgat cagtggccat cggggatggg cttcaactac ttctacggct tcatgggcgg
     2821 cgagactgat caatggacgc cgtatctgtt ccgcgaccac tcccagattt ttccgtgggt
     2881 cggcaagtcc gattacaacc tgatcaccga catggcggac gaggcgatca agtacatgcg
     2941 ggagctcaac gccaacgcgc ccgaccagcc gttcttcctc tattacgttc ccggcgggag
     3001 ccactcgccg cacaatccga agaaggagtg ggtcgagaag ttcaagggca aattcgacat
     3061 gggctgggaa aagctgcgcg agcagatctt cgccaaccag aagcggttgg gggtcattcc
     3121 agcgaatgcg cagctcaccc cgtggcccga tgatttgccg aaatgggaga cgctgtcggc
     3181 cgacgagaag aagctatttg ctcgacaggc ggaagtgttc gccggctaca ccgcctatac
     3241 cgataacgag atcgggcgtg tgatccagga ggttgagaac ctgggcaagc tcgacaacac
     3301 cctgatcatc tacatcgtcg gcgacaacgg cacgagtccc gaaggctcga agctcggcac
     3361 gcccaaccag tacaccgcgt acaacggcat tctggacgtc ccgatcgacg cgcaactgca
     3421 gttctacgac gtctggggct cgtcggcgac ctatccgcac atggcggtgg cgtggtcatg
     3481 ggcattcgac acgccgttca agtggaccaa gcaggtggcg tcgcatttcg gcggcacgcg
     3541 gcagggcctg gcgatctcgt ggccggcccg catcaaggac gtcggcggca tccgccacca
     3601 gttccaccac atcatcgaca tcgtgcccac gatcctcgag gtgacgggga tcaaagcacc
     3661 ggaatacgtc aacggtatca agcagaagcc gatcgaaggc gtgagcatgg cctatacctt
     3721 cgacaaagca aacgccaacg cgccctcgac gcacaagacc cagtacttcg agatggcgag
     3781 caaccggggg atctaccatg acggctggta cgccaacact acgccgcctg ttccgccgtg
     3841 ggtgctcaac gcgccgatgc ctcccgtgaa cgagtacaag tgggaactct ataatctgac
     3901 cgaggactat acccaataca atgatctcgc ggcgaaaatg cccgacaagc tcaaggagat
     3961 gcaggcgctg tttgttcagg aggcgagcaa gtacaacgtc tttcccctcc agaactccac
     4021 gttcgcccgt gcgatcgatc cgcgaccgag tgacaccgcg ggacagaccg tcttcacgta
     4081 ttcgggtatg atgcccggca tcccaagcgg caacgcgccg agcatcttga accgatcgtt
     4141 cacgatcacc gccgaggtcg acgttccgca aggcggcggc aacgggatga tcgtcaccga
     4201 gggcgggcgc tggggcggct tcggcctcta cctgctcaac ggcaagccgg tgttctccta
     4261 caacctgctc atgctcctct atccccgctg gacaggcaac gagccgctga cagcgggcaa
     4321 gcacacgatc gtgttcgact tcaagtatga cggccccggc atcgcgaaag gcggcaccgg
     4381 cgtgctgaag gtggacggac gggaagttca cacgctgagc attccgaaga cgattccgtt
     4441 cctgctgccg gcggacgaga ccttcgatgt cggggtcgac acccgcacgg gagtgaacga
     4501 cctcgactac aaagtaccgt tccagttcaa cggcacgatc aacaaggtga cgttcaatct
     4561 cgggccggtc caactgacga gcgatgagca tcaggccatt cggcacgctc gcgccaaagc
     4621 gaacgactag gaagcaaggc ggattggcga tggcgcctgc gtgcgggcat cgccaaacac
     4681 ggaccacgcc cttgcagagg cacctgaatc agtcagctcg aggaggaatg cagatgacca
     4741 aatcaattgg tgcagcagtg tgtgccatgg cgttgatctc ggccggcggc gtcgccgcgc
     4801 agggcatgct gttggaattt gcggccgaca aggtgatcaa gaaatacgag tcggcgactt
     4861 gcgagcagtt gaaggtgcag aagaacgagc cgcgaacaga gagggaaaag atggccatcg
     4921 atttcctgcg ccacgattcg caagcacgcg tggccttcat cgacaagatc gcggcgccgg
     4981 tcctgaacaa gctgtttgaa tgcgacttga tcccgtgaac gggtcagcgg gatcgggagc
     5041 aggcgcttct gtcagcaatc gaccgaggag atgatgcgat gagccagagc cacttcaccc
     5101 tgcgctttcc tttgaaatca tcggcggacg cgaaagcagt ggcgcaggag ctgccgccgt
     5161 tgatgccgga tctgttccgg gcggaagacg cgatcggcac ggtgcactat tcacgtttca
     5221 cggtgctgag cgaccgtacg ctgctcttcc tcggcgactt cgacggcgat ttcggtccgc
     5281 tcatgcgcga gctggggcgg agcaccgggc aggtgttcga caaaatcttc aagcatgtga
     5341 gcgatccgcc accgacgccg gtcgcgagca acccggacgc attcacggaa tgggccgcgg
     5401 aacatctgct cagtcccgcc tatgtcttca gcgcctatcc cggcccgaca gtcaaggaga
     5461 tcaaggcgat ggctgccgcc gccggcgtcg agggccggac gcaattgctt ccgttcctgg
     5521 tgatcctgcc cgcgcgcggc cgcgtggcgt tcgccgagat gcagctgatc ctgcggacga
     5581 tggcgatgac cggcaaggtc gaccgcgaca tgaccagtgt cgggacgccg catttcgcgc
     5641 agttcgtgcc gctcgaggat cagcagcttg gcttcttcac cgtctatgac ggcgcattcg
     5701 acaaatacat catggatttc acccgcaaca tcggcgaggt gttcgatgtc atcttcaaat
     5761 tcaccaagga cccgccgccg tcgccgtgcc gaaagcacgt gcaggagttc atcgacttcg
     5821 ccgcgggcgc aagccgagct ccgatcggct tctaccaggc ctatcccggg ctgagcaatc
     5881 aggacatcaa ggcgctgatc gccgacagcg ggacgaaggc ggcttgagcg ccgcacgcat
     5941 cgtggagggg tgccgtgagc caggacagca acacgcggtc gcccgccgga gagcggcgcg
     6001 aagggctgtt tcaccacttg tttgaacgga tcgagggccg cgtccactcg acggccgccg
     6061 ccaagaccgc cctcgacctg gaggacatcc aggggctcat tctgcgcggc taccgcatgc
     6121 cgatggtgcg acatttcctg ctgaaggtga cggcgccggc ggcggcccgc acgctcatcg
     6181 gtcgcctcgt cagcggcgac gaagccgatg cgccgcagat cacgacggca aaggactggc
     6241 atgtcgggtt cgaaccgggt cccggcgacg atccggcgtc ggtcccgcgc tgcaagcccg
     6301 actattgcct caacgttggc atcacctggc cgggcttgct ggccttggag gtgaaggatc
     6361 gcatcccgaa cctctcgttc aagtcgttca aagcgttcat ggccggcgcg gccgcgcggg
     6421 ccgagctggt cggcgacacc ggcgccaacg ggccgcagca ttggatcggc ggcttcggca
     6481 ccggcaacga tcacgtgctg gtgacgctgc acgccatcag cccggaggcg atgaggagct
     6541 acagcgatcg cctgtccgag ctgctctcgc agggcgccgc cttcgaggaa atctggcgcg
     6601 gcgacgggat ggccttgatg gagatgcagg acggcaagcc ggtgcccacg accaaggtgc
     6661 atttcggcta caccgacggc atcagcatga ccacgatccg cggcgggccg gagcactaca
     6721 cgcgcgatca tcagcgcccg tgcgagccct ggctgttcgt gctgctcgac gaggccgaga
     6781 actacgtcct tccggggccc aagcagctga gccgcaatgg cagcttcgcc gtgttcaaga
     6841 tgatccgcac cgatgtcgtc ggcttcgaga acttcctgca gtcgcacaag gatcgcatcg
     6901 atccggagct gctcgccgcc aagctgtgcg gacgctggcg caacggcgtg ccgctcatgc
     6961 tctcgcccga caccgacaag cccgccggcg ggctcgcgcc cgacgcgctc aacgacttcg
     7021 agtatgtgaa cgccgacggg tccggcgatc ccaagggttt gcgctgtccg gtcggcgcgc
     7081 acatgcggcg gatcaatccg cgcggccagc cagtcatcgg ccagggcctg cccggcggca
     7141 gcaacaatac gcaccggctc atccgtcgcg gcatgcccta cgggcctact tacgatccca
     7201 ccaaaccgta tgacggaatg gaacgcgggc tcctaggcta cttcatcaat tccagcatcg
     7261 agaaccagta cgaattcgtg ctaagccagt gggtgaacga cgccgaattt gccggatccg
     7321 ttcgcctgcc tcccaaatcg agggatccga tcatcgggac gcagaatccc gaggagagca
     7381 tttttgtcat tccgcaagcg aatggcgcag agccgatcaa gatcaccggc ttttcgagct
     7441 tcatcacaac gcgagcggcg gcttactgct tcttgccgag catcagcgcg atgaagctca
     7501 tcgcgggtct ctgattcgga gctggcgctc attgccttcg ggggcgagca tcgaggcagc
     7561 tatcatgtcc ttcctgacca ggctcttggg actcaccgtc agcgacctcg agcgcagcct
     7621 cgcgacggaa ccggccgtga tcgcggagat cagcgaaaag atcattttga tgcaaaaaaa
     7681 cgcggcggcg gagcaacgtc ggccaatctg ccgcggaacc cacgccaaag gcgtgtgcgc
     7741 acgggctcaa ttcgaagtat tcgacgtcag cgtcggacgc gaccgcgcgc tgacggcacg
     7801 gcttgccaag ggcatctacg cgaagccggg aatctatccc gctgtcgtgc ggtttgcgaa
     7861 tgcggatccg aaggtcaatt ccgatttcaa gtcagacgtc cggtccttgt cgttctccgt
     7921 cgagctcgcg ccgggcggcg cagctgcctg gggcgcgaag atttcgtgcc aggactactc
     7981 gatgcagaac gcgaccacgc tccccctcaa cgacgctcac gcgtttctcg tggcgatgga
     8041 ggtactcacg gcgtcgagcc cggccaaggc cattcattcg ctgccctggc gggacaaatt
     8101 gatattcgcg agaaccatga tcctcgccca gctgcaggca catcaaacgg tcaagcccta
     8161 tcagcaattg cgctactgga gcacggtgcc gttccgtcac ggaccggcag atgtcgtcaa
     8221 gtattcggcg acgccgagtg ttgataatcc cgcgcggccc ttgcaaaaga agtatccgaa
     8281 cgccctgcaa gatgagttcg ctcgacacct gaacgaggat gccaaaatga gctcgttcga
     8341 tttcgcctta cagttcctcg acaccgagcg gatgacctac tggggcaagc gtcgcgacgc
     8401 cggcttctgg atcgagaacg cgagtgtgga gtggaaagaa acgcaagcac cgtttcacag
     8461 ggttgcacgg ctcagcctag tgccaaaatc aaagctcgag caagacgtca gcgagtcctt
     8521 gtacttcgat gtgaccggaa attccctgcc cgacagcacg cccctgggga gcatcaatcg
     8581 cgcgcgatgg cctgcggagc tggcgagcag aaaggcgcgc caaacgcatg tacaatctgc
     8641 gatgaaaaga ttggcgcatc aatcgggaaa cctgccatga caccgcgcag cccgctgatc
     8701 tgttgtgtcg gcgcggccag tctgatgttc ggcacgttct gcctcgtgca aaagggcgcg
     8761 gcgcatgcgg agctcggaag ttgcggcccc tattccggcc tgccatcgga cgacaccgac
     8821 accgccggga tggtgttcat ccgcggcggg acattcgtca tgggctccga gcgccaccgt
     8881 cgggaagagc gcttcaccca cgtcgtgcgc gttgacgggt tctggatcga tcgccacgag
     8941 gtgaccaacg cgcagtttcg ggaatttgtc accgcgacgg gatacgtgac gttggccgag
     9001 cgcggccttg accctgcgac gcgccccgaa atgtccaagg agctgctcgc ccccggctcg
     9061 gtggtcttca tcccgcccgc caacgtcgcg agcggcggcc ggatcacgca gtggtggcaa
     9121 tacgtggcgg gcgcgaattg gcgtgagccg acaggtcccg gcagctcgat cgcgggcaag
     9181 gacaatcatc cggtcgtgca tattgcctac gaagatgcgc tcgcctacgc gcgctggcgc
     9241 ggacgcgagc tgccgaccga ggcgcaatgg gaattcgccg cgcgcggcgg gcgcgacggg
     9301 gaggatgact ggtcgagtgc tttcgatgcc gagggcaagc cgatcgccaa cacctggcaa
     9361 ggcgtcttcc cagtctacaa ctcgaacgag gacggctacg tcgggaccgc gccggtcggc
     9421 tgcttcaagc cgaacggcta cgggctctac gacatgatcg gcaatgtctg ggagtggacc
     9481 agcgattggt atcggccggg ccacccgcgc gaggcggccg tcaacccgag tggacccgaa
     9541 ttgcaggaca ttccgctggc gcggggccag tcaccgagcc gtgtcatcaa gggcgggtct
     9601 tatctgtgcg cctccaacta ctgctcgcgc tatcgacccg ccgcgcgtca gccgcaggag
     9661 atcgacctcg gcgcggcaca tctgggcttt cgaacggtct caaatagatc agacagcgag
     9721 acacgctagc gggacagttc gtctaccagt ggcacttctc ggaccccagg cccgtccgct
     9781 ttcgcaccgc tattcgggct aagaccgcgt caacccgcgc gttacgttgt tccgatttat
     9841 gagtacacgc cctagggcgt cccgcagcga cgttggttat tttgtaatca tccgtcgcgg
     9901 cggggcgctc ggcgtctgtt ccgccggcga gatcacgaag gtgacccagg cattccagcc
     9961 atccggccgg ttctggttgg cgaattccca gtagcttttg agattgagat agccttgcgt
    10021 ctcagtagtc aggggaatga tgaatccgag ctgcggcccg gcgcccacca cctgcgactg
    10081 gaagcaaccc acccggtcgc cggcgccggt atcgcaaccc aactgtctgt agacatagcc
    10141 gacgacgcca acctggactt gcttggtcaa gaattgcgac gcaccccaat cgaaatgcat
    10201 gtcgacgccg ttctgatatt gcgtcgattg attggtgaaa ttgtaggtga atccgagcac
    10261 gcccgagaat tcgtgcccgg tctgcgggtt gagataggtg tagccggcgc ccgcgtcgat
    10321 tccgctgtgc ccgatgccga tgttcgacag tcgtgtggaa tcgtaagccc cgaccggcac
    10381 atcccccatc acataggtca tgtaattgtg aacgccgttg ttccagcgca gcgtcgccag
    10441 cggaaacaca tcgccgaagc cgcttactga gtcgctgatg ctgtcgaagc gcgagaatgg
    10501 gagtgtgccg agcggagtag tcaacgcgcc tgtaaccgac cccgctaaga aggtgtcagc
    10561 gcggccatat attccaatg
//
LOCUS       DATLJT010001520         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1980643,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001520 DATLJT010000000
VERSION     DATLJT010001520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980643"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VM910_34825"
     CDS             <1..274
                     /locus_tag="VM910_34825"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67720.1"
                     /translation="QGKRMERASKRAGISPAVNFHALRHTFASHLAMAGCPLMVIAEA
                     LGHNDVTMVTRVYGHLSPSFSAAQIREKAPRYGIKPGNVRPLARST"
     gene            360..1028
                     /locus_tag="VM910_34830"
     CDS             360..1028
                     /locus_tag="VM910_34830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67721.1"
                     /translation="MAKDSESPFDPQRDCVYNALWGDERLLNHSTWGLYWILLHSPEV
                     LAQIKASLGRFFALHNLTRMLLEMRICSAARGLSKEGEIVFLLPWEFWKDVTLQEEAG
                     CIRILKASDPDFARLAMDVFVLREQFNNHFRTKPVVVPSESNVIAMTPAATVVPYLDP
                     DEAGIPYSWREIRRAVIKQWPTTIEGIPTPVIVRHLQTVGVLSGYGDHRPTVERALDR
                     RPRK"
     gene            1150..1509
                     /locus_tag="VM910_34835"
     CDS             1150..1509
                     /locus_tag="VM910_34835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67722.1"
                     /translation="MARGEVTGRKPAVSAERSKRDIPNKFKTKAKGQFEATPHAQPDP
                     IASRQRPPARAPPTCLFTIKSFCLGHGISESFYHELRKQGLGPKEMRLGARVFITHEA
                     AAEWRAEREAACSTTAE"
     gene            1671..2195
                     /locus_tag="VM910_34840"
     CDS             1671..2195
                     /locus_tag="VM910_34840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67723.1"
                     /translation="MHHMASATANRKAEFKRKHKPRINNDGICLAAKRGMRAARAYLA
                     GTFDTLKEAAEAHGTCVPYVESGIILVKFERLTGDWSLTWEVVHGWRSMLEAAEQAQT
                     LVRLLDAFELAPSATKDAFFTITGTADLSTPEKCLEYGKKIGPGRVWDLMVSPLVGID
                     SNGKDVHEPGVVGT"
     gene            2266..2592
                     /locus_tag="VM910_34845"
     CDS             2266..2592
                     /locus_tag="VM910_34845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67724.1"
                     /translation="MTNQETITDDASPDDEAEQGWLRVLDRIKKLRTAIDGLADEVEE
                     VSQRTAARYRALGLPFLTFGGWIWIPKREGREWIASRVKRRNPRRQRRQAIPASLNII
                     APRAPP"
     gene            2703..3353
                     /locus_tag="VM910_34850"
     CDS             2703..3353
                     /locus_tag="VM910_34850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67725.1"
                     /translation="MVTHPSTTISPSSAAGATAPGSLLARYISAHVRKGEQVEERANQ
                     AREKAEQHFIAADARQIIENVTGFFSTLHEWARAEISRAGNHKTRIGTSLREHTPGAT
                     PIRRKSARINPTAQKEWSMHLKALADRAHLLRAWRRWHREQLKEALAGVHGAVLERLM
                     AKLKDLRSARELVAFIEAQDWACVDSDTRAIALFEIDRAIVALRERTDQGRADRAR"
BASE COUNT          743 a         1110 c         1081 g          628 t
ORIGIN      
        1 ccaggggaag cggatggagc gcgcctcgaa gcgcgccggc atctcgcccg cggtcaactt
       61 ccacgcgctc aggcacacct tcgcctcgca tctggcgatg gccggctgtc cgctgatggt
      121 catcgccgag gcgctggggc ataacgatgt gacaatggtc acgcgcgtct acggccattt
      181 aagtccgagc ttcagcgccg cacagattcg cgagaaggcg ccgcgctacg gcatcaagcc
      241 cgggaacgtc cggccgcttg ctcgatcgac atgactgggc tgagattgat ggcccgggta
      301 ccttaggagc gccccggggc caatgtgcgt tttcccaccg ccaagcaggt ccacagcaca
      361 tggccaagga tagtgagtca ccattcgatc cgcaacgcga ctgcgtttat aacgcgctct
      421 ggggtgacga gcggctgctc aatcactcga cctggggcct gtactggata ttgctccact
      481 cccccgaagt tctcgcccag atcaaggcct ccctagggcg cttcttcgcc ctccataatc
      541 tcacgcggat gttgctcgaa atgcgcatat gctcggcagc gcgcggccta tccaaggagg
      601 gggagatagt tttcttgctg ccttgggagt tctggaaaga tgtcacactg caggaggagg
      661 ccgggtgcat ccgcatacta aaggcctcgg atccggactt cgctcgccta gcaatggacg
      721 tgtttgttct gcgcgaacag ttcaacaatc acttccgcac caagcccgta gtggtgccat
      781 cggaatcgaa cgtgatcgcg atgacgccgg cagcgaccgt cgtgccctat ctcgatcccg
      841 acgaagccgg cattccgtac agctggagag aaatcaggcg ggccgtcatc aagcagtggc
      901 ccaccacaat tgagggcatc ccaacacccg tcatcgtaag gcacctgcaa accgtggggg
      961 tgctctccgg atacggagat catcgcccta ccgtcgagcg cgccctagat cgccgccctc
     1021 gcaagtgacc tggtcatttt tggtcattct tggtcaccgg gcgcgccccg ttcttgacct
     1081 aacttgcaca tcgcttcgga aagcctctgc gatcatatgt agctccactt cgaacggtgg
     1141 agcactgcaa tggcccgcgg agaggtaact ggtcgcaagc cggccgtcag cgctgagcgc
     1201 agcaagcgcg acataccgaa caaattcaag accaaggcca agggccaatt cgaggcgaca
     1261 ccgcacgccc agcctgatcc gatcgcgagc aggcagcgcc cgccggcgcg agctccgccg
     1321 acgtgcctat tcacgatcaa gagcttctgc ctgggtcacg gaatttcgga atctttctat
     1381 cacgagctgc gtaagcaagg tctcggcccc aaggagatgc gcttgggagc gagggtcttc
     1441 atcacccacg aagctgcagc cgaatggcgc gccgaacgcg aagcggcgtg ctcgaccact
     1501 gcggaatagg gggcgagcag cgctccgaag aacgctgtcg ctgagtcaaa ggagttcgag
     1561 agttccgggg atctcccggg aaaccacggc ggggttggcc cgaccgccgg cggaatgggg
     1621 tctcagcgat gcaagcgctg tcgccctggt ggccttagag gagtatgacc atgcaccaca
     1681 tggcatctgc gaccgccaat cgcaaggcgg aatttaagcg gaagcataag ccccgcatca
     1741 ataatgacgg catctgcctc gcagccaagc gcggcatgcg cgccgctcgg gcctatctcg
     1801 cgggcacctt cgacaccctc aaggaagcgg ctgaggcaca cggaacgtgt gttccctacg
     1861 tcgagtccgg aatcattctc gtgaaattcg agaggctgac cggcgattgg tcgctcacct
     1921 gggaggttgt gcacggctgg aggtcaatgc tcgaggcagc ggagcaggcg cagaccctgg
     1981 tcaggctcct cgatgccttc gagctggcac cgtcggctac caaggatgcg ttcttcacca
     2041 tcaccggtac cgccgacctc agcacgcccg agaaatgtct cgagtacggc aagaagattg
     2101 gtcccggccg cgtctgggac ctgatggtct cgccgctggt cggcatcgat tccaacggca
     2161 aggacgtcca cgagcctggc gtcgtcggca cgtaattgcc gagagtgtgt gtgactggcg
     2221 ccgttcgcgc caatgcgggc ggcgctcttt ctggaggatt agaccgtgac gaaccaagag
     2281 accattacag acgatgcatc cccggacgac gaggctgagc agggatggct aagagttctt
     2341 gatcgcatca agaaactcag aaccgccatc gacggtctcg ccgacgaggt ggaggaggtt
     2401 tcgcagcgca cggcggcgcg atatcgagct ttgggcctgc cgtttttaac atttggcggc
     2461 tggatctgga ttccgaagcg cgaggggcgt gagtggatcg ccagccgcgt gaaacggcgc
     2521 aacccccgtc gccaacggcg gcaagcgatt cccgcttcgc tcaacatcat cgcgccccgc
     2581 gcgccgccct aaaagttgcg cgccgaccct ggcaagaggc cggcgcgcga taggggctac
     2641 ttcggcaaaa gagagaaccg accagtagcc cgcttcgaac ttcttagtca agcgaggttt
     2701 ccatggtcac ccacccttcc acaacaatca gtccctcctc agccgccggc gctaccgcgc
     2761 ctgggtcatt actcgcccgc tacatcagtg cgcacgtgcg gaagggcgag caagttgagg
     2821 aacgggctaa tcaggcgcgc gaaaaggccg agcaacactt cattgccgcc gacgcccgac
     2881 aaataatcga gaacgtcacc ggcttcttct cgacccttca cgaatgggcg cgtgccgaaa
     2941 tttctcgcgc cgggaatcac aaaactcgaa tcggaaccag cctgcgggag cacacaccgg
     3001 gggcaacacc gatccgtcgc aagagcgcac ggattaaccc aaccgcacag aaggagtggt
     3061 cgatgcacct gaaggcgctc gccgaccgcg cccatctcct gcgcgcctgg cgtcgctggc
     3121 accgcgagca attgaaggaa gcgctcgccg gtgtgcatgg cgctgtgctc gagcggctga
     3181 tggccaaact caaagatctg cgcagcgccc gcgagctcgt cgccttcatc gaggcgcagg
     3241 attgggcctg cgtcgacagc gacactcgcg cgatcgcgct gtttgagatc gaccgcgcaa
     3301 tcgtcgcttt gcgcgagcgc accgatcaag gacgcgctga tcgagctcgg tgagcctttg
     3361 accgtatttc aggtgatccg cgggatcatc accaagtttc ccgtcgtagc gggaaaaccc
     3421 gccgagtcag ttcggtgaaa cataggagcg tgaaatggaa aacgtacaat gtaagctgcc
     3481 ggctctgccg acactcgatg tcgagcaggc tatacacgga caacgtatag agttcgtgga
     3541 caaggaatgg ttcttgaacg ac
//
LOCUS       DATLJT010001521         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_603281,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001521 DATLJT010000000
VERSION     DATLJT010001521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603281"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VM910_34855"
     CDS             complement(<1..1284)
                     /locus_tag="VM910_34855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVI67726.1"
                     /translation="MSNKRPEPKIVKPEDIDPQHDWNRPLMALGQMAVDFEERVDFRR
                     LHKYRLGRTRQALARSGLGAILTFDQHNIRYISSTVIGEWARDKMTRYCLLTGPGDPY
                     VWDFGSAAKHHRIYAPWLEDDHCRAGLLGLRGAIHPEAGLFRKAAGEIKAILEAEGVA
                     DTPLGVDMIELPMLFELQRLGIEVRDGQQMMLDARELKSQDELMLLNTSAAMVDGTYQ
                     RIAEALKPGVRESQIVALANHDLYSKGSDCVEAINAISGERCAPHPHNFTDRLIRPGD
                     QAFFDIIQSYMGYRTCYYRTFNVGRATDAQRDAYNRAREWIDEAIAMIRPGVRSDEVA
                     ACWPTADKIGFTSEMEAFGLEFGHGLGLGLHERPIISRLNSMEDPMEIKAGMVFALET
                     YCPATDGISAARIEEEIVVTGTGCKVITLYPAETLP"
     gene            complement(1287..1583)
                     /locus_tag="VM910_34860"
     CDS             complement(1287..1583)
                     /locus_tag="VM910_34860"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67727.1"
                     /translation="MSATELYNAPQSTCSQKVRLTLAEKGPSFKEHRLKLFAGDQLKP
                     EYLKLNPNGVVPTLIDDGTPIIDSSVIIGYLDEVFPSPQLSPSDPAEAAPARNG"
     gene            complement(1681..2280)
                     /locus_tag="VM910_34865"
     CDS             complement(1681..2280)
                     /locus_tag="VM910_34865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI67728.1"
                     /translation="MHEQFRCPHPSPIPRGVHHLALCTEDMKATIDFYVDVLGMPLVH
                     AMKVPPGLGTGSGNRGNPPYERVRHYFFDMGDDSLLAFFEIPKGKEPAGNRNAIGAMQ
                     HCAFVVTAERFREVEARLVSRRIDYIGPIPQLPGLLGIYFYDPNGIRLEFACQPDDGE
                     APSVVPCVMQTRAEARSELASLPGVTPEWIEKRLAALPD"
     gene            complement(2312..>2573)
                     /locus_tag="VM910_34870"
     CDS             complement(2312..>2573)
                     /locus_tag="VM910_34870"
                     /inference="COORDINATES: protein motif:HMM:NF024975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI67729.1"
                     /translation="IADKAFDSDERVIRPLQSAAKSAVIPPRKHRLASRDFDRDLYKT
                     RHLIENFFCKLKQFRAIATRYDKTARNFLAALHLAAASIWLN"
BASE COUNT          456 a          751 c          837 g          529 t
ORIGIN      
        1 cggcaaggtc tcggccggat agagcgtgat caccttgcag cctgtcccgg tgaccacgat
       61 ctcctcttcg atgcgtgcgg cggaaatgcc gtccgtcgcc ggacagtagg tctcgagcgc
      121 gaacaccatg ccggctttga tttccatcgg gtcttccatg gagttgagcc ggctgatgat
      181 cggccgctcg tgcaacccca gcccgagccc gtggccgaac tcgagtccga acgcctccat
      241 ctcgctggtg aaaccgatct tgtcggccgt cggccagcac gccgcaacct cgtccgagcg
      301 cacgcccggc ctgatcatcg cgatcgcctc gtcgatccac tcgcgggcgc ggttgtatgc
      361 atcccgctgc gcgtcggtag cgcgcccgac gttgaaggtc cggtaataac aggtgcgata
      421 acccatgtaa gactggatga tgtcgaagaa tgcctgatcc cccggccgga tcaaccggtc
      481 ggtgaagttg tgcgggtgtg gcgcacagcg ctcgcccgag atcgcgttga tcgcctcgac
      541 acagtcggag cccttgctgt agaggtcgtg gttggccaag gcgacgatct ggctctcgcg
      601 cacaccgggt ttgagcgcct cggcgattct ctgatacgtc ccgtcgacca tcgccgccga
      661 cgtgttgaga agcatcagtt cgtcctggct tttgagctcg cgcgcatcga gcatcatctg
      721 ctggccgtcg cgcacctcga tgccgagacg ctgcagctcg aacagcatcg ggagctcgat
      781 catatcgacg ccgagcggcg tgtcggcgac accttcggcc tctagtatag ccttgatctc
      841 accggcggcc ttgcggaata ggcccgcctc cggatgaatg gcgccgcgca gaccgagcag
      901 gccggcgcgg caatgatcgt cctccagcca tggcgcgtag atgcggtgat gcttggccgc
      961 cgagccgaag tcccacacat acggatcgcc gggaccggtc aacaggcaat accgagtcat
     1021 cttgtcgcgc gcccactcgc cgattaccgt cgaagaaatg tagcggatgt tgtgctgatc
     1081 gaaggtgaga atggcgccga ggccggatct ggcaagcgct tggcgcgtgc gtccgagccg
     1141 gtacttgtgc agccgccgga aatcgacgcg ttcctcgaaa tcgaccgcca tctggccgag
     1201 cgccatcagc ggcctgttcc agtcatgctg cggatcgatg tcttctggtt ttacgatttt
     1261 cggctcggga cgcttgttgc tcatcgctaa ccgttcctgg ctggtgcggc ttcggcggga
     1321 tccgatgggg agagctgcgg cgacggaaac acttcatcaa ggtatcctat gatcaccgac
     1381 gagtcgatga tcggcgtccc gtcatcaatc aatgtcggga cgaccccgtt tggattgagc
     1441 ttgagatatt cgggtttgag ttgatcgcct gcgaagagct tcaggcggtg ctctttgaat
     1501 gacggcccct tttctgcgag cgtgagccgt accttctggc tgcatgtcga ctgaggcgcg
     1561 ttgtacagct cggttgcgga catcgtgaca gctctttaag ctcgagcagc agcatttatg
     1621 cgagggcgcg cttcgccccg agacgtgggc gagatcggcg ccgcatgcgg atcagttcag
     1681 tcagtctggc aaggcggcga ggcgcttttc gatccactca ggcgtgacgc ccggcaagct
     1741 agcgagttcg gagcgcgcct ccgccctggt ctgcatgacg cacgggacca ccgagggcgc
     1801 ctcgccgtcg tctggctggc aagcaaattc gaggcggata ccgtttgggt cataaaaata
     1861 gattccgagc agccccggga gctgtgggat cggcccaata tagtcgatac ggcgcgatac
     1921 cagccgcgcc tcgacctcgc ggaagcgctc ggcggtgacg acgaatgcgc aatgctgcat
     1981 ggccccgatc gcgttgcggt tgccggcggg ttccttgcct ttcgggatct cgaagaacgc
     2041 cagaaggctg tcatcaccca tatcaaagaa gtaatgacgc acccgctcat agggtgggtt
     2101 gccgcgattg ccggacccgg tgccgagacc gggcggcacc ttcatggcat gaacgagcgg
     2161 catgcccagt acgtcgacat agaagtcgat cgttgccttc atgtcctcgg tgcagagtgc
     2221 caggtgatgg acgcctcgtg gaatgggtga cgggtgcgga caccgaaatt gttcgtgcat
     2281 ggcgtttcct cggtttggct aaggcgtgtc gtcaattgag ccagattgaa gcggcggcga
     2341 ggtggagggc ggcgaggaaa tttctcgcgg ttttgtcgta gcgcgtggcg atggcacgga
     2401 actgtttgag tttgcagaag aagttctcga tgaggtggcg cgtcttgtag aggtcgcgat
     2461 caaaatcgcg gctggcgagg cggtgttttc ggggcgggat cacggcggat tttgcggcgc
     2521 tttgtagggg tcggataact cgctcatcgg aatcaaaggc cttgtcggcg atc
//
LOCUS       DATLJT010001522         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3589884,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001522 DATLJT010000000
VERSION     DATLJT010001522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3589884"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VM910_34875"
     CDS             <1..153
                     /locus_tag="VM910_34875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67730.1"
                     /translation="VDKILRGAKPADIPVEQPTKFDFVINLTTAKALGLKIPEAVLLR
                     ADEVIE"
     gene            150..1127
                     /locus_tag="VM910_34880"
     CDS             150..1127
                     /locus_tag="VM910_34880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67731.1"
                     /translation="MRRRAFITLIGAAAAWPLTARAQQPAIPVIGYLSVGSPESDASR
                     LTGLRRGLNQTGYVEGRSLVIEYRWAGNQADRLPALAADLVQLQVTAIVAAGVLPALA
                     AKAATASIPIVFIVAADPVQFGLVASLNRPGSNLTGFNDFAGEVGAKGLALLHELLPG
                     TATIGVLGNPNNPIFELTTRDLLAAAAIIGLKVEVLKAGTDSEIDAAFTSAVQAQTGA
                     LLVEADPLFITRIERVIALAARHGIPTMYSFREYVVAGGLISYGTSLIEDWRQIGLYT
                     GRILKGEKPADLPVIQATKIQLIINLKTAKTLGLEVPATLLALADEVIE"
     gene            1124..2128
                     /locus_tag="VM910_34885"
     CDS             1124..2128
                     /locus_tag="VM910_34885"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67732.1"
                     /translation="MSANMKRREFITLLGGAAAAWPLAAGAQQPSSTVRRIGFLSPGV
                     ARTMAVRGLVEAFRQGLKEYGWVEGQNISVEYRFAEGKEEALAGIAAALVQSRLDVIV
                     ADGTAAIQAAKNVTQTVPIVMAVSNDPVGTGLVASLNRPGGNITGLSILTGELVSKWL
                     QLLTEIVPGLARVAVLSNPLNPSSAPLLEQTKAAAQSLGVEIHVAEVQGPDRFESAFA
                     AIKAARAGALIVLPDGMLYSQHPRIVTFSAASHLPALFGQKEVVEAGGLIAYGPNIPA
                     SFRRSAAYVDKILQGAKPADLPVEQPTIFELVVNLETARAIGLTVPASILLRADEVIE
                     "
     gene            2125..>2433
                     /locus_tag="VM910_34890"
     CDS             2125..>2433
                     /locus_tag="VM910_34890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67733.1"
                     /translation="MKRRKFITLLGGAMAAWPLAARAQQPRERMRRIGVLMHLAADDP
                     EGQSRVAAFLQGLQEAGWAVGRNVNIDVRWAAGEADRFRKYAMELVALAPDVILTSAP
                     P"
BASE COUNT          482 a          702 c          782 g          467 t
ORIGIN      
        1 gtcgacaaga ttctgcgcgg agcgaaacct gccgacatcc cagtcgagca gccgaccaag
       61 ttcgatttcg tcatcaatct caccaccgcc aaggcgctcg ggctcaaaat accggaagcc
      121 gtcctgctgc gcgccgacga ggtgattgaa tgaggagacg cgcgttcatc acgctcatcg
      181 gcgcggcggc cgcgtggccg ctcacggcgc gcgcacaaca gccggccatt ccagtgatcg
      241 ggtatttaag cgtcgggtcg ccggagtccg acgcctcccg gctcaccggc ctccggcgag
      301 gtctgaacca aaccggctac gttgaaggcc ggagcttggt gatcgaatat cgttgggcgg
      361 gaaaccaagc tgaccgactg cctgcgttgg cggccgatct ggtccaactt caagtgactg
      421 cgatcgtcgc ggcgggggtg cttcctgctc ttgcggcgaa ggctgcgacc gcaagcattc
      481 caattgtgtt catcgtggcc gctgaccccg tgcagtttgg gcttgttgcg agcctcaatc
      541 gaccgggaag caacctaacg ggcttcaacg acttcgccgg tgaagtcggg gccaaggggc
      601 tcgcgctgtt gcacgagttg ctgccgggca ctgcgacgat cggcgttctt gggaatccga
      661 acaatccgat atttgagcta acaacgagag acctgctggc agcggctgct atcatcgggt
      721 taaaggttga ggtattgaaa gcaggtaccg acagcgaaat cgatgccgcc ttcacgagtg
      781 cggtccaagc acaaaccggg gcgctgctcg tcgaagctga ccctcttttc atcacccgga
      841 ttgagcgggt cattgcactc gcggcacgcc atgggattcc gaccatgtac tcgtttcgag
      901 agtacgtagt ggcgggcggg ctgatcagtt acggaaccag cctgatagag gattggcgac
      961 agatcggact ttacacgggt cgcattctca agggtgaaaa gcccgccgac ctgccagtca
     1021 tacaggccac aaaaatccag ttaatcatca atctcaagac cgccaagaca ctggggctgg
     1081 aagtgccagc aacgctgctc gcgctcgccg acgaggtgat cgaatgagcg caaacatgaa
     1141 gcgccgcgag ttcatcacgc tgctcggcgg cgcggcggcg gcgtggccgc tcgcggcagg
     1201 ggcgcagcag ccatcttcga cagttcgccg tattggtttt ctctcgcccg gggttgcacg
     1261 cacgatggcg gtgcggggtt tggtggaggc atttcgtcag ggattgaagg aatacggttg
     1321 ggtcgagggg cagaacatca gcgtcgaata tcgttttgcc gagggtaaag aggaggcgtt
     1381 ggcggggatc gccgccgcgt tggtccaatc acggctggat gtcatcgtgg cggacggtac
     1441 cgcggcaatc caggcggcaa agaatgtgac gcaaaccgtg ccaatcgtaa tggcagtgag
     1501 caacgatcca gttggaactg gcttagtcgc gagcctcaac cgacccggcg ggaacataac
     1561 cgggttgagt atcctcacgg gggaactggt cagcaagtgg ctccaactcc tgaccgaaat
     1621 tgttcccggg ctcgcacgag tggccgtttt gtcgaatccg ttaaatccga gtagcgcccc
     1681 gttgctggag caaaccaaag cagcagcgca atcgcttggc gttgagattc atgtcgcgga
     1741 agtgcaggga ccagaccggt ttgagagcgc gttcgccgcg atcaaggcag cgcgcgctgg
     1801 cgctttgatc gtcttgccgg acggcatgct ctacagtcaa cacccgcgta tcgtcacgtt
     1861 ctcagctgcg tcgcacctac cggcgctctt tgggcagaag gaggttgtgg aggctggcgg
     1921 tctcatcgcc tatggcccga atattcccgc tagtttccgg cgctcggctg cctatgtgga
     1981 taaaattctc caaggcgcca agcctgccga tttgccggtc gaacagccga ccatattcga
     2041 gcttgtggta aatctcgaga ctgccagagc gattggtctc acagttccag catcgatact
     2101 gttgcgcgcc gacgaggtga tcgaatgaag aggcgcaagt tcatcacgct gctcggcggc
     2161 gcgatggcag cgtggccact cgcggcgcgc gcgcagcagc cgcgcgagcg tatgcggcgc
     2221 atcggcgtgc ttatgcactt ggccgctgac gatccggagg ggcaaagccg cgtcgccgca
     2281 tttctgcagg gtctacagga ggcgggctgg gcagtcggcc gcaacgtcaa catcgacgtg
     2341 cgatgggcgg cgggtgaggc cgatcgcttt cgtaagtatg caatggaact ggtcgcgctc
     2401 gcgccggatg tcatcctgac atctgcccca ccg
//
LOCUS       DATLJT010001523         1564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_371356,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001523 DATLJT010000000
VERSION     DATLJT010001523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371356"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VM910_34895"
     CDS             <1..711
                     /locus_tag="VM910_34895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HVI67734.1"
                     /translation="VELVLAVDVSYSMDPDEQALQREGYIAGFTSREFMQALRSGQHG
                     KVAVTYFEWAGPYDQKIVVPWRLIDGPESADAFVNEIARASYRRASRTSISSALNFAK
                     PLFDGSGYRGFRRVIDVSGDGANNSGPFVTIARDDVLAAGMTINGLPIMLKRPNSFTM
                     DIDNLDIYYEDCVIGGPGAFVIPIREREQFKEATRTKLVLEIAGRMPERRVMPAQARA
                     PRISCTIGEQMWRDRWGN"
     gene            945..>1564
                     /locus_tag="VM910_34900"
     CDS             945..>1564
                     /locus_tag="VM910_34900"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVI67735.1"
                     /translation="MQTKRLLLGMGVGLAVTLVPAALVRAQSQPPAALTGHVTSEAQG
                     AMEGVVVSAKKAGSTVTVSVVSDAQGRYSFPANRLSAGKYSLKIRAIGYDLTAPATAD
                     VAEEQTATVDLKLRKAKNVASQMSNAEWMMSLPGTEDQKAFLLNCVGCHTLERIVRSS
                     HDADEWTQVIVRMNGYGPVSQPIKPQRMLDPARSGSPEQFRKQAEYL"
BASE COUNT          308 a          484 c          492 g          280 t
ORIGIN      
        1 gtcgagctcg tattggccgt cgacgtgtcc tactcgatgg acccggatga gcaggcgctg
       61 cagcgcgagg gctacatcgc cggcttcacg tcgcgcgagt tcatgcaagc gctgcgtagc
      121 ggccagcacg gcaaggtggc ggtcacctat ttcgagtggg cgggacccta cgatcagaag
      181 atcgtcgtgc catggcggct catcgacggc ccggagagcg ccgatgcatt cgtgaacgag
      241 attgcgcgcg cgtcttatcg tcgtgcctcg cgcacctcga tttcgagcgc gcttaatttt
      301 gccaaacccc ttttcgacgg cagcggctat cgcggctttc gacgcgtcat cgacgtctcg
      361 ggcgacggtg ccaacaacag cggcccgttc gtcaccatcg cgcgcgacga cgtgcttgct
      421 gccggcatga ccatcaacgg cctgccgatc atgttgaagc ggccgaactc gttcaccatg
      481 gatatcgaca acctcgatat ttattatgag gattgcgtaa tcggcggccc cggtgccttc
      541 gtcatcccca tccgcgagcg cgagcaattc aaggaagcga cgcgtaccaa actggtgctg
      601 gagatcgctg gccgcatgcc cgaacgacgc gtgatgccgg cgcaagcgcg agcccctcgc
      661 atctcctgca ccatcggcga gcagatgtgg cgggatcgct ggggaaacta ggccgcggaa
      721 ttcctcaagc tgcgattgac gaccggcgtt cgtgccgagc gggcaagcgc gcattgacta
      781 gagcggttgc tggcgaggtt tcggcgccgc ccgcggagag agatcgcgtc cacagcttcg
      841 tgcaagcctg agcaggcact ggatttcgat aaccacgcat tctacgatgg tccatctggg
      901 aggtgtgtcc tcgaattgtg acccaactcc gtcgggagga atcgatgcaa acaaaacgtt
      961 tactgctggg catgggcgtc ggtttggccg tgacgctcgt gcccgcggcg cttgtgcgag
     1021 cgcaaagcca accgccggcc gcccttaccg gacatgtcac ctccgaagcg caaggcgcga
     1081 tggagggggt ggtggtcagc gccaagaagg cgggctcgac ggtcaccgtc agcgtcgtca
     1141 gcgatgcaca aggccgctac agctttccag ctaatcgatt gagcgccggt aagtattcgc
     1201 tcaagatccg cgccatcggc tacgacctca ccgcgccggc aaccgcggac gtcgccgaag
     1261 aacagaccgc caccgtcgat ctcaaattgc gcaaggcgaa gaatgtggcc tcgcagatga
     1321 gcaacgccga atggatgatg agcctaccag gcacggagga tcagaaagcc ttcctgctca
     1381 attgcgtcgg ttgccatacg ctcgagcgca tcgtgcgctc gagccacgat gcggatgaat
     1441 ggacccaggt catcgtgcgc atgaatggtt atgggccggt gagccagccg atcaagccgc
     1501 agcgcatgct cgatccggcg cgctcgggca gccccgagca attccgcaag caggccgaat
     1561 acct
//
LOCUS       DATLJT010001524         6296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4277665,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001524 DATLJT010000000
VERSION     DATLJT010001524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277665"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VM910_34905"
     CDS             <1..927
                     /locus_tag="VM910_34905"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67736.1"
                     /translation="ARAQQGERIRRVGMISGIADEAGTRARLAAFLPALQRLGWTEGR
                     NVQFEYRWGNGDTETLRKDAAELAALAPDVIVATGGATTTYMLQATHTVPIVFVIVPD
                     PVGSGFVKSLSRPGGNATGFMQFEYSLSGKWLELLKQIAPNVTRAAVLWDPSVPAGIG
                     QYAVIQSVAPALGTDVFPIKSTNTGDMERDVADFAQTANGGLIVTANPSTVFHREQIA
                     ALAARHGLPAVYSTRDFVVSGGLASYSADFPDQYRRAADYVDRILKGEKPGDLPVQAP
                     TKYEMVINLKTAKNLGLEIPPTVLARADEVIE"
     gene            945..1925
                     /locus_tag="VM910_34910"
     CDS             945..1925
                     /locus_tag="VM910_34910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67737.1"
                     /translation="MKLPRRNFLHLAASAAALPALSRLARAQAYPSRAARIIVPFAPA
                     GSTDIVARLMGQWLSERLSQQFVVENRPGAGSNIGTEAVVAAPADGYTLLLAGPANVI
                     SMTLYDKLNFSFTRDIAPIASIMRFPFVLEVHPSVPAKTVPEFMAYAKANPGKLNMAS
                     AGTGTATHLTGELFKMMTGLNILHVPYRGAGPALIDMIGGQVQLMFDTVPGSIEHIRA
                     GRLRALAVTGAARSDVLPDIPTVGDFVPGYESSGWHGVAAPKNTPTEIIDKLNKEINV
                     ALTEPRIKERIADLGGTVFAGSPSDFSKLIVEETKKWAKVIKFSGAKPEG"
     gene            2170..>6296
                     /locus_tag="VM910_34915"
     CDS             2170..>6296
                     /locus_tag="VM910_34915"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI67738.1"
                     /translation="MKKQLEVRTRELAEALDQQTATSQVLSVISSSPTDVQAVLDAVV
                     ASAARLCEALDAVIVLRDGDVVVNRAHSGPLGAANLGQRLPLNRRWVTGRAVLEARTI
                     HVPDLLDSDEYPEGKEMALRWGHRATLVVPLLRDATAIGAILVRRREARPFTDKQIEL
                     VNNFAKQVVIAIENTRLLNELRESLQQQTATADVLKVISRSTFDLQAVLDTLVESAAK
                     LCEADMGIIDRQQGDVYRAVSFFGHSSEMVGFMQDHPLEISRGNVAGRAILERRTIQI
                     PDVQVDPEFTFVEAARIAGVRTNLGVPLLREGSPVGVIVLQRRKVAPFTDKQIELVET
                     FADQAVIAIENVRLFDEVQARTEELSESLQQQTATADVLKVISRSTFDLPTVLSTLVE
                     SAARLCEADKAAILRPTERDASYYVAASYGHTPEFDGHVKTQTFAPGRGGVVGRVLLE
                     RKSVQIPDVLADPEYAFRDLARIGGFRTILGVPLLREGMPIGLLLMHRAAVRPFTQKQ
                     IELVETFADQAVIAIENVRLFDEVQARTRDLSESLEQQMATAEVLKVISRSKFELQPV
                     LDTLVESATRLCDAERTAILLRDGNVYRIAARYGFSLELEEYQKQHPIAPGRDTLTGR
                     VAQTGAVVHIPDALADPEYTYFEAQKLGGWRAMLGVPLLREGSCVGVMAMTRDTPGPF
                     TDKQIELVTTFADQAVIAIENVRLFDEVQARTRELTESLEQQTATSEVLQVISTSPGE
                     LEPVFQALLANAARICEGKFGSLYLYDGGKFRVGALHNAPAAFAEFRRREPVFDPPSG
                     TGLAQIVATKRTVHTPDITLEKGYIDRNPIIVASVELAGFRTVLAVPMLKDDSLIGCV
                     NIYRQEVRPFTDKQIELVTNFARQAVIAIENTRLLNELRESLEQQTASSEVLQVISSS
                     PGELEPVFQAMLENATRLCKAKFGTLLLSEGDTLRMVAMHNVPPAFAEKRRREPVIRP
                     HPETALARVARTKQAVQIADARAGPYSSDLVDLAGARTILGVPMLKDEELVGAIGIYR
                     QEVRPFTDKQIALVTNFARQAVIAIENTRLLNELRESLQQQTATADVLKVISRSTFDL
                     QVVLDALTESAARFCEAQMSAIARQKDAGSYCYATSYGVSADSGAYLKSVVIRAGRGT
                     VVGRALLEGRIVQVADVLADTEYTWTEAQERTGFRTVLAVPLLREGIPIGVFVLARSV
                     VRAFNDKQIELVTTFADQAAIAIENVRLFDEIQDKNRQLAEASEHKSQFVSSMSHELR
                     TPLNAIIGLTEMMVKNASRFGTEKAQEPLQRVNRAGTHLLGLINQVLDLSKIEAGKLE
                     LNLQTVQLKPLINEVIGTAGQLAEQNKNRLVVDAHENLGALTVDPMRLRQILLNLL"
BASE COUNT         1282 a         1884 c         1939 g         1191 t
ORIGIN      
        1 gcgcgcgcgc agcagggcga gcgcatccgg cgggtcggca tgatctcggg tattgcagac
       61 gaggctggca cgcgggcgcg cctcgcggca tttctgccgg cgctgcagcg attaggctgg
      121 actgagggtc gcaatgtgca gttcgaatat cgctggggca atggcgatac tgagaccctg
      181 cggaaagatg cggcggaatt ggcagcactt gctccggacg tgatcgtagc aacgggcggc
      241 gcgaccacga cgtatatgct tcaagctacc catactgtgc cgatcgtatt cgtgattgtc
      301 cctgatccag tcggctccgg tttcgtcaag agtctgtcgc ggccaggcgg caatgccacc
      361 ggcttcatgc agtttgaata cagtttgagt ggaaaatggt tagagttgct taaacagatt
      421 gcgccaaacg tcacgcgcgc ggcggtcctg tgggatccct cggtcccggc tgggatcggc
      481 cagtacgccg tcattcagtc ggtggcaccc gcgctcggga ccgacgtctt tcccatcaag
      541 tcgaccaaca ctggcgatat ggaacgtgac gtcgcggact tcgcacagac cgcgaacggc
      601 ggtttgatcg tgacggccaa cccgtcaaca gtttttcatc gcgagcaaat agccgctctt
      661 gcggctcgac acgggctccc cgccgtttac tccactcgcg attttgtggt cagcggcggc
      721 ctggcgtcct attcggccga ttttcccgat caatatcggc gcgcggccga ttacgtcgat
      781 cgcatcctca agggtgagaa gccgggtgac cttccggtgc aggcgccgac caagtacgag
      841 atggtgatca acctcaagac cgcgaagaac ctcggcctcg aaatcccgcc gacggtactc
      901 gcgcgcgccg acgaggtgat cgaatgatgt gagggaggtt caaaatgaaa cttccccgcc
      961 gaaattttct gcatctggcc gccagcgcgg cggcgctgcc ggccctgtca cggctcgcga
     1021 gagcgcaggc ctatccatcg cgggcggcgc gcatcatcgt gccgttcgct ccggccggtt
     1081 cgaccgacat cgtcgcgcgc ctgatgggtc aatggttgtc ggagcggctc agccagcaat
     1141 tcgtggtcga gaaccgcccc ggtgctggca gcaacatcgg cactgaggca gtcgtggctg
     1201 cgcctgcaga tggttatacg cttctcctgg cagggccagc gaatgtgatt agcatgacgc
     1261 tctacgacaa actcaacttt agtttcacgc gcgatatcgc gccgatcgcc agcatcatgc
     1321 gttttccttt cgtcctggaa gtgcatccct cggtgcccgc caaaaccgtt cctgagttta
     1381 tggcctatgc caaagccaac cccggcaagc tgaacatggc atcggcaggg acgggaaccg
     1441 caacccattt gactggcgaa ctcttcaaga tgatgacagg tctcaacata ctccacgtgc
     1501 cgtatcgcgg tgcggggccc gccctcatcg acatgatcgg cggacaggta cagctcatgt
     1561 ttgatacggt gccggggtca atcgagcaca tcagggctgg caggttgcgc gcgttggccg
     1621 tgaccggcgc agctcgttcg gacgtgttgc cggacatccc gacagtgggc gacttcgttc
     1681 ctgggtacga atcgagcggc tggcatggcg tcgccgcgcc caagaacacg ccgactgaaa
     1741 tcattgacaa gctcaacaag gaaatcaacg tcgcccttac cgaaccccgg atcaaggaac
     1801 gaatcgccga tcttggcggt acggtgtttg cgggctcacc gtccgacttc agcaaactca
     1861 tcgtcgagga aacgaagaaa tgggccaagg tgatcaagtt ctcgggcgcg aagccggaag
     1921 gatgactgga ccgcgacata ttccataacc acccgttcag cgaatggcgg cgacggggtg
     1981 atcgagtgag ccgcgtgcgg ttgtgttaag attgccgggc tcgaggattg gcgggccgac
     2041 gggaagacct gtgcgcgtgc cgacgaggtg atcttgtctc gcaaaggcgc aaatagccca
     2101 acgcggcttc gttcgagtgg aacgaaggcg agagcgcgcg ttagcaacgg gcccaattca
     2161 ctggtcgagt tgaaaaagca actggaagtg cgcacccgtg agctcgccga ggcactagac
     2221 cagcagacgg cgacttccca ggtattgagc gtcatctcga gctcgccgac cgacgtccaa
     2281 gccgtgctcg acgcggtcgt cgcgagtgct gctcgtctct gcgaggcatt ggacgccgtg
     2341 atcgtgcttc gcgacggcga cgtcgtagtg aaccgtgcgc actccgggcc actcggtgct
     2401 gctaacctcg gccaacggct accgctcaac cgccgctggg tgaccggccg ggcggtgctc
     2461 gaagcgcgga cgatccatgt cccagacctc ctcgatagcg acgagtatcc tgagggcaaa
     2521 gagatggctc tccgctgggg tcatcgagcg accctggtgg tgccattgct ccgcgacgcg
     2581 accgctattg gcgctatcct cgtacgccgc cgggaagccc ggccatttac cgacaagcag
     2641 atcgaacttg tgaacaattt tgccaagcag gtcgtcatcg ccatcgagaa cacgcgcctg
     2701 ctcaacgagc tgcgcgaatc gctgcagcag caaaccgcca ccgccgacgt gctcaaggtc
     2761 atcagccgct caaccttcga tcttcaggcg gtgctcgata cgctagtcga gtcggcagcc
     2821 aagctgtgcg aagccgatat ggggatcatc gaccggcagc agggcgacgt ttatcgggca
     2881 gttagtttct tcgggcactc atccgaaatg gtagggttca tgcaagatca tccgcttgag
     2941 attagccgcg gaaacgtcgc agggcgggcc attctcgagc gacgcaccat ccagatccca
     3001 gacgttcagg tcgatcccga attcaccttc gtggaagcgg cgcgcatcgc cggcgttcgc
     3061 accaatctcg gcgttccatt gctgcgggaa ggatcgccgg tgggcgtgat cgtcttgcag
     3121 cgccggaaag tcgcgccgtt caccgacaag caaatcgaac tggtcgagac cttcgcggac
     3181 caagcggtca tcgccatcga gaacgtgcgc ctattcgacg aggtgcaggc gcgcacggag
     3241 gaactgagcg agtcactcca gcagcagacc gccaccgccg acgtgctcaa ggtgatcagc
     3301 cgctcgacct tcgatttgcc gacggtgctc agcacgctgg tcgaatcggc cgcgcggttg
     3361 tgtgaggcgg ataaggcggc gatcctccgt ccgaccgaga gagacgcgag ctactacgtc
     3421 gcggcgagct acggtcatac gcctgagttc gatggacacg tgaaaaccca gacgtttgcg
     3481 ccgggacgcg gcggcgtcgt cgggcgagtc ttgctggagc gcaaatctgt tcaaatcccc
     3541 gatgttcttg ccgatcccga atacgccttc cgtgacctcg cgagaatagg cggctttcgt
     3601 acaattctcg gagttccgct cctgcgagag ggaatgccga ttggccttct cctcatgcac
     3661 cgtgctgccg tgcgtccgtt cacgcagaag caaatcgaat tggtcgagac cttcgcagac
     3721 caggccgtga tcgcaatcga gaacgtgcgg ctgttcgacg aggtgcaggc acgcacgcgc
     3781 gatctctccg aatcgctgga gcagcagatg gcgaccgcgg aggtgctgaa ggtcatcagc
     3841 cgctcgaagt tcgagctcca gccggtgctc gacaccttgg tcgagtcggc aacacgtctt
     3901 tgtgatgcgg agcggaccgc catccttctt cgcgatggca atgtctatcg tatcgcggcg
     3961 cgttatggct tttcgctcga actggaggaa tatcaaaaac agcatcccat tgccccaggc
     4021 cgagacacac ttaccggtcg ggtcgcgcag accggcgcag tggtacacat tcctgatgca
     4081 ctggccgatc cagagtacac ctatttcgaa gcccaaaaac tgggtggttg gcgtgcaatg
     4141 ctgggcgtgc cgttgttgcg agagggaagc tgtgtcggcg tcatggccat gacaagggac
     4201 acgcccgggc catttacaga caaacagatc gagcttgtaa cgacgtttgc cgatcaggcg
     4261 gtgatcgcca tcgagaacgt gcgtttgttc gacgaggtgc aggcgcgcac gcgcgaactc
     4321 accgaatcgc tggagcagca gacggcgacc tctgaggtac tgcaagtcat ctccacttcg
     4381 cctggtgagc tagagccggt gttccaggcc ctgctggcga acgcagcgcg catctgcgaa
     4441 ggcaagtttg gctcacttta tctctacgac ggaggcaaat ttcgcgtcgg cgcactacac
     4501 aatgcgccgg ccgcctttgc ggagtttcgc aggcgggaac cggtatttga tcccccatcg
     4561 gggacgggcc ttgcacagat cgtcgcaaca aagcggacgg ttcatactcc cgacataacg
     4621 ctggagaagg gctatattga tcgcaatccg atcatcgtcg ccagtgtcga actagccggc
     4681 tttcggaccg tgttggccgt tccgatgctc aaggacgaca gtctgatcgg ctgcgtcaac
     4741 atctaccgcc aggaggtgcg cccgttcact gacaaacaga tcgagctggt cacgaacttc
     4801 gcgcggcagg cagtcatcgc catcgagaac acgcgcctgc ttaacgagtt gcgcgaatcg
     4861 ctggagcagc agacggccag ctccgaagtc ctgcaggtga tctccagctc gcccggcgag
     4921 cttgagcccg tgttccaagc catgttggag aacgcgacgc ggctctgcaa ggccaaattc
     4981 ggaacactgc tgttaagtga aggggacaca ttgcggatgg tcgcgatgca caatgtgccg
     5041 cctgcgtttg ccgaaaaacg gcggcgtgaa ccggtcatcc gtcctcatcc ggaaactgcc
     5101 ctggctcgtg tcgccagaac aaagcaggcg gtccagatcg ccgatgcaag ggccgggcca
     5161 tattcctcag atcttgttga cctcgccggc gctcggacga ttctcggcgt cccgatgctg
     5221 aaggatgagg agctagtcgg ggctatcggt atctaccgcc aggaagtgcg gcccttcacc
     5281 gacaagcaaa ttgcgctagt cacaaacttc gcccgccagg cggtcatcgc gatcgagaat
     5341 actcgtctgc tcaatgagct acgtgaatcg ctgcagcagc agaccgccac cgccgacgtg
     5401 ctcaaggtca tcagccgctc gacgttcgat ctgcaggtcg tgctcgatgc gctgacggag
     5461 tcggcggcgc gattttgcga ggcgcaaatg tccgcgattg cgcgccaaaa ggatgcaggc
     5521 tcttattgct acgctacgag ctacggcgtc agtgctgact ccggtgccta cttgaagagc
     5581 gtcgtgatca gggcggggcg gggaaccgtt gtagggcggg cgctgctgga aggcaggatt
     5641 gtccaagttg cagacgtttt ggccgatacg gagtacacat ggactgaggc ccaggaaaga
     5701 acgggttttc gcactgtgct cgcggttccc ctgttgcgcg agggaattcc gattggcgtg
     5761 ttcgtactgg cgcgttcagt cgtacgagcg tttaacgaca aacagatcga gctagtgacc
     5821 accttcgccg atcaggcggc tattgcgatc gagaacgtgc ggctgttcga cgagattcaa
     5881 gacaagaatc ggcaactggc ggaggcgagc gagcacaagt cgcagttcgt ctccagcatg
     5941 agccacgagc tgcgcacgcc gctcaacgcc atcatcggac tgaccgagat gatggtcaaa
     6001 aacgcgtcac gcttcggcac ggaaaaggcg caggagccgc tgcagcgcgt gaaccgcgct
     6061 ggtactcacc tccttggcct catcaaccag gtgctcgacc tgtcgaagat cgaggccgga
     6121 aagctcgaac tcaacctaca aaccgtgcag ctaaaaccgc tgatcaacga ggtcatcggc
     6181 accgccgggc agcttgccga gcagaacaag aaccgcctcg tcgttgacgc ccacgagaac
     6241 ctgggcgcat tgaccgtgga ccccatgcgg ctgcggcaaa tcttgctcaa ccttct
//
LOCUS       DATLJT010001525         4545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3130748,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001525 DATLJT010000000
VERSION     DATLJT010001525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130748"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1613
                     /locus_tag="VM910_34920"
     CDS             195..1613
                     /locus_tag="VM910_34920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HVI67739.1"
                     /translation="MIAAFDEMKRADGKVRPAYGELVRWLAETTADVLHHRRREAELL
                     FRRIGITFAVYGDAEARERLIPFDIIPRIVSAAEWRLLEKGLSQRVKSLNLFLRDVYG
                     AREILRAGVVPEDLIFRNPSFRPEMSGQQVPHDIYIHIAGIDIIRVEADTFYVLEDNV
                     RTPSGVSYMLENREIMMRLFPELFARHRIAPVENYPDVLLATLKSAAPASAARDTTVV
                     LLTPGIYNSAYYEHSFLADKLGVELVEGRDLFVKDEIVYMRTTQGPRQVDVIYRRIDD
                     DFLDPLTFYPDSALGVPGLMSAYQAGNVTLANAVGTGIADDKAIYSFMPEIVKFYLGE
                     QPLLKNVPTWRCREDDHLTYVLGHLNELVVKEVHGSGGYGMLIGPTASKAEVEQFRAK
                     LKTAPQNFIAQPTLALSTCPTCVEEGVAPRHVDLRPFVLTGRDRTHIVPGGLTRVALK
                     KGSLVVNSSQGGGTKDTWVLDG"
     gene            1728..2669
                     /locus_tag="VM910_34925"
     CDS             1728..2669
                     /locus_tag="VM910_34925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HVI67740.1"
                     /translation="MLSRTADNLFWLSRYVERAEFLARILDATQRLTTLPLAYVGSSN
                     EWESAVATAGCATAFFKAYDEANEENVTDFLAFSAANQTSIRGCFELARSNARAVRTA
                     LTVEMWDAINSTWLELKRYGNGHTSREEFSRFLRWVQESSLHYDGSAYRTMLRNDAYW
                     FSRLGVYIERADNTARILDVKYHLLLPEHERVGGPLDYFQWAAILRSVSALTAYHWVY
                     RDSLKPWLIADLLILKDEMPRSLASCYEAIVRNLDAIAESYGRQGPAQRQARGIRTRL
                     QNSRMDGVFQRGLHEFIGEFISENNRLGFAITEQFLV"
     gene            2720..3547
                     /locus_tag="VM910_34930"
     CDS             2720..3547
                     /locus_tag="VM910_34930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI67741.1"
                     /translation="MRIRILHTTSYCYDAPPKGVTQMLRLTPRNHDGQYVVRWRIELS
                     EDCRLHQREDAFGNITHSFTAEGLFNELSIAVEGEVDVHDTNSVVRGAVERFPPSLFL
                     RETALTQPDAAIIDFAQSVRAGAGSETLPLLHRLTVATNREITFDTDPTHSATTAAEA
                     FTLRRGVCQDLTHIFIAAARQLGIPARYVGGHFYRADGVTAQDAGHAWAETYVEHLGW
                     VGFDPANGIGTTEAHVRVAVGLDYLGAAPVRGVRYGGNGETLRVAVHVDQADRQSQN"
     gene            3617..4366
                     /locus_tag="VM910_34935"
     CDS             3617..4366
                     /locus_tag="VM910_34935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HVI67742.1"
                     /translation="MTYCCGILVRDGFVMIADTRTNAGLDNVSTFRKLHVYTKAGERI
                     LALASSGNLSLSQSVRSTLIEGVENPETGELETLMNAPTMFQAAQRIARVVRSIQNAE
                     GKAVEAAAIGHDVAFLFGGQIQGRRTRLFMIYSAGNFIECTTDTPYLQIGEHKYGKPV
                     LDRAISFNTDLYDALKIGLISMDSTMRCNLGVGLPIDILVTHCDSYTAEVNYRIEPGE
                     PYFQDLRERWSAALRAAQIAIPRPPYRSPVE"
BASE COUNT          882 a         1391 c         1330 g          942 t
ORIGIN      
        1 catccaaatc ggcaattttc tgccctttgc gaaaccgtat ttgcctatag tttaggcggc
       61 cggccgttct actcagacgt aactctgcaa ttttgctgag tttttgttgg cgtaaagctt
      121 gcagcttttt agtcggagct gccgatggcg acatacccat tgagaagtgg tgcccgacag
      181 ccggggttcc ggagatgatt gccgccttcg atgagatgaa gcgagccgac ggaaaggtgc
      241 gtccggccta tggtgagctc gtgcgctggc tggccgagac gacggccgac gtgctccacc
      301 atcgccggcg cgaggccgag ctgttgtttc gccgcatcgg catcaccttc gcagtctacg
      361 gcgatgccga agcacgggag cggctgatcc cattcgatat cattccccgc atcgtgtccg
      421 ccgccgagtg gcggctgctc gaaaagggtc ttagccagcg cgtcaaatcg ctcaatctgt
      481 ttctccggga tgtctacggc gcacgcgaga ttttgcgggc cggcgtggtg cccgaggatc
      541 taatcttccg taacccctcg tttcgtccgg agatgagcgg acagcaagtg ccccacgaca
      601 tctacatcca catcgccggc atcgatatca tccgcgtgga ggccgacact ttctacgtgc
      661 ttgaggataa tgtgcgcacg ccgtccgggg tctcgtacat gctggagaac cgcgagatca
      721 tgatgcggct gttccccgaa ctgttcgcgc gccaccgcat tgcgccggtg gagaactatc
      781 cggacgtctt gctggctacc ctcaaatcag cggcgccggc ctcggccgcg cgcgacacga
      841 ccgtggtgtt gcttacaccc ggcatctaca actcggccta ttacgagcat tcgttcctgg
      901 ccgacaaact tggcgtcgag ctggtggaag ggcgtgactt attcgtaaag gacgagatcg
      961 tgtacatgcg tacgacccag gggccgcgcc aggtcgacgt gatatatcgg cgcatcgacg
     1021 acgattttct cgatcccctc acgttctatc ccgacagcgc gcttggcgtg cctggcctga
     1081 tgtccgccta tcaggccggc aatgtaacgc tcgcgaatgc ggtcggtacc ggcatcgctg
     1141 acgacaaggc tatctacagt ttcatgccgg agatcgtgaa gttctatctc ggcgagcagc
     1201 cgttgctgaa gaatgtgccg acgtggcggt gccgggagga cgaccatctc acctatgtgc
     1261 tcggccatct gaatgagctg gtcgtgaagg aagtgcacgg ctccggtggc tacggtatgc
     1321 tgatcgggcc gactgcatcg aaagctgagg tcgagcagtt ccgcgccaaa ctgaaaaccg
     1381 caccgcagaa tttcattgcg cagccgacgc tcgcgctatc cacgtgcccg acttgcgtcg
     1441 aggagggcgt agcgccacgc cacgtcgact tgcgtccgtt cgtgctgacc gggcgtgatc
     1501 gcacccatat cgtgccgggc ggcttgacgc gtgtcgcgct caaaaagggt tcgctggtag
     1561 tcaattccag ccaaggcggg gggaccaagg acacttgggt tttggacgga tgagggcggc
     1621 aagtctcgca aaagccttgg ccgcgaacag tagagatccc gcgtcggatc gagagactgt
     1681 ggactactgt ttccgcgcgc gcctcgccac catgatagga aaaaggcatg ctctcgcgaa
     1741 ccgccgataa cctgttttgg ctttcgcgtt acgttgagcg ggcagaattt ctcgcgcgaa
     1801 ttctcgacgc gacccaacgg ctcaccacgc tgccgctcgc ctatgtcggg agcagcaatg
     1861 aatgggaatc agcggttgcg actgccggct gtgcgaccgc ctttttcaag gcttatgacg
     1921 aagccaacga ggaaaatgtc accgactttc tcgccttctc cgccgccaat cagacctcga
     1981 tccgcggctg ctttgaactt gcgcgtagca acgcgcgtgc ggtgcggacc gcgctgacgg
     2041 tggaaatgtg ggacgccata aacagcacct ggctcgagct caagcgttac ggcaatggac
     2101 acacctcgcg cgaggagttt tcgcgtttcc tgcgctgggt gcaggaatcc tcgctgcatt
     2161 atgacggctc cgcgtacaga acgatgctgc gcaatgacgc atattggttc tcgcggctcg
     2221 gcgtctatat cgagcgcgcc gataacaccg cgcgcatcct cgacgtgaaa taccacctgc
     2281 tcttgccaga gcatgaacgc gtgggcgggc cgctcgacta tttccagtgg gcggcgatcc
     2341 tgcgctccgt ctccgcgctt accgcctatc attgggttta tcgcgacagc ctcaaaccct
     2401 ggctgatcgc cgatctcctc attctgaaag atgaaatgcc gcgctcgctc gcttcttgtt
     2461 acgaggcgat cgtgcgcaat ctcgatgcca tcgccgagtc ctacggccgg cagggtccgg
     2521 cccagcgaca ggcccgcggc atccgcacca ggctgcagaa cagccgcatg gacggagtct
     2581 tccaacgcgg gttacacgaa ttcatcggcg aattcatcag cgagaacaac cgtctgggtt
     2641 ttgccattac cgagcagttc ttggtatgaa ccaacgtagt gccgttctgg tgcatgctct
     2701 gtgctagcgt cgcacgacca tgcggattag gattctgcac accaccagtt attgctacga
     2761 cgcgccaccc aaaggcgtga cgcagatgct ccgccttacc cctcgcaatc acgatggaca
     2821 atatgtggtc cgctggcgaa tcgagctctc tgaggattgc cggctgcatc agcgcgagga
     2881 cgcgttcggc aacatcaccc attcgttcac ggctgaggga ctcttcaatg aattgtcgat
     2941 cgcggtcgag ggtgaggtcg atgtccatga taccaacagc gttgtgcgtg gcgcggtcga
     3001 gcgctttccg cccagcctat tcctgcgcga gaccgcgctg acacagcctg acgcggcgat
     3061 catcgacttc gcccaaagcg tgcgcgcagg cgcgggaagc gaaacgctgc cgctgctgca
     3121 tcgcttgact gtcgcgacca atcgcgaaat cactttcgac accgatccga cccattccgc
     3181 gaccacagcg gcggaagcct tcacactacg tcgcggcgtg tgccaggatc tcacgcatat
     3241 tttcatcgca gcggcgcgcc agctcggcat tccagcgcgc tatgtcggcg gccatttcta
     3301 ccgcgctgac ggcgtaaccg cacaggacgc cggtcacgcc tgggcggaaa cctatgtcga
     3361 gcatctggga tgggtcggct tcgatccggc caatggcatc ggcaccacgg aggcccacgt
     3421 gcgggtggcg gtgggtctcg attacctcgg cgccgcgccg gtgcgcggcg tccgctacgg
     3481 cggtaacggc gaaacgctca gggtcgccgt tcacgtcgac caggcggacc ggcagtcgca
     3541 aaactagcgg tttcttgccg gacccggtgc tgcgccgcta taagattgac agctcggtta
     3601 tacccccctc ctttccatga catattgctg cggaattctg gtgcgtgacg gctttgtaat
     3661 gatcgcggac acccgcacca atgcaggcct cgacaacgtc tcgacattcc ggaagctgca
     3721 cgtctacacc aaggcgggcg aacgcattct ggcgctcgcc tcctcaggta atctgtcgct
     3781 cagccagtcg gtgcgttcga cgctcatcga aggcgtggaa aatccggaaa ccggcgaact
     3841 cgagacgctg atgaatgctc caaccatgtt ccaagccgcg cagcgcatcg cccgcgtcgt
     3901 ccgcagcatc cagaacgccg aaggcaaagc ggttgaagcc gccgctattg gccacgatgt
     3961 cgccttttta ttcggcggtc agattcaagg ccgccgcact aggctgttca tgatctactc
     4021 ggccggcaat ttcattgaat gcaccaccga cacgccttat ctgcagattg gtgaacataa
     4081 atacggcaag ccagtgcttg accgcgccat cagcttcaac accgatcttt atgacgccct
     4141 caagatcgga ctgatctcaa tggattctac gatgcgatgc aatctcgggg tcgggctgcc
     4201 gatcgacatt ttggtgacgc attgcgacag ctataccgcc gaggtgaact accgcattga
     4261 gcccggggag ccttatttcc aggatttgcg cgagcgctgg tcggctgcct tgcgcgcagc
     4321 tcaaatcgcc attccacgtc cgccctaccg tagcccggtc gaatagtccc gcaaatctga
     4381 tgtctgctta tggcaacaaa gctgacattg agcgagcgcc cttgctgtaa tgcaacggtt
     4441 cccattcgat atggtgggct ggcgtcgccg ggcttatgga ggctcatgaa gcgacgagtt
     4501 tatcacgcgc cgacgaggcg atcgaatagc ttgtcatttt gcaac
//
LOCUS       DATLJT010001526         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4050100,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001526 DATLJT010000000
VERSION     DATLJT010001526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050100"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VM910_34940"
     CDS             <1..518
                     /locus_tag="VM910_34940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI67743.1"
                     /translation="GALLGVPVGTYLLSRLDPVTTRWIISFFVFALLILLLSGWRYRG
                     KDHVALSVGIGALAGFCSGLAQTGGPPIVGYWLGRPIASVVARANIVLFFGASDFFSL
                     VSYAVSGLITRDAILFALVVGPVYALGIKFGVSLFGRASEALFRAICYALIAFAVVFG
                     PPVLDGVLRAG"
     gene            554..1495
                     /locus_tag="VM910_34945"
     CDS             554..1495
                     /locus_tag="VM910_34945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI67744.1"
                     /translation="MVDRRDVLKLGLSAAAALTAPAAFAQAAKPRTRIVFLGTKGGPR
                     VGLGRSNPANLVVVNDTPFVIDCGMGVSRQLVAAGVPLETVRYIFISHHHSDHNLEYG
                     NLVYNAWATGLSTPIHSFGPKGIEAMTKTFFELNRFDIETRIEDEGRPDLRKLLIAKD
                     ITEDGVVLQTADVKVTAFRTPHPPITDNFAYKFETPDGVVVFSSDTNYNPQLAEFAKR
                     ADVLVHEALYLPWVDRLVTRVKNGATLKKHLLESHTAAEDVGRIADAANVKVLVMSHL
                     VPDDPDVTDDDWRSEAAKNFKGRIVVARDLMELRLPL"
     gene            complement(1517..2917)
                     /locus_tag="VM910_34950"
     CDS             complement(1517..2917)
                     /locus_tag="VM910_34950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67745.1"
                     /translation="MTMTRRGFLSASAALASAPAWSGGAFSAALPRDADIVVIGAGAA
                     GIAAARRIMAANRKVIVVEAADRVGGRCITDTSSFEVPFDRGARWMHNPDSNPMIRLA
                     RGAGFEIVTAPGQKIRIGRRNARAGETEDFLATLVRANRAIDDASRRLDGSCAAALPK
                     DIGDWAGAIEFTLGASFAGKDLKELSTVDKARAGDRGSVFACRQGLGTLIAKLSEQVP
                     VTLSTPVNRIVWSTRDVGVETPAGKITARAAVITVSTNVLAAGNIKFNPDIPKRALDA
                     AAKLTLGSYDHIALVMPRNPLGLSRDDVIIEQSTSARTALLYANIGGSSLCSVDVAGS
                     FGRDLSAEGEAAMVAFALEWLTKLFGSDAAAAVKKSGATRWNASPYVLGAISAAAPGA
                     QSSRKALLEPIGSLFLAGEATHETLWGTVDGAWESGERAAEAALRRIGALKDTEPLAG
                     APEPRRRQRRGAPQIN"
BASE COUNT          480 a          966 c          937 g          556 t
ORIGIN      
        1 tcggcgcgct gctcggagtc cccgtcggga cctacctcct gagccggctc gatcccgtga
       61 ccacgcgctg gatcatctcc tttttcgtgt ttgcgctcct gatcctgctg ctgtccggct
      121 ggcgctatcg cggcaaggac catgtcgcgc tgtccgtcgg catcggcgct ctcgccggct
      181 tctgttcggg tctggcgcag actggggggc cgccgatcgt cggctactgg ctgggacgtc
      241 cgatcgcttc ggtcgtcgcg cgcgccaaca tcgtgctttt cttcggtgcg tccgatttct
      301 tttcgctcgt gagctatgcc gtgagcgggc tgatcacgcg cgatgcaatc ctgttcgcac
      361 tggtcgtcgg cccggtctat gcgctcggca tcaagttcgg agtctcatta ttcgggcgcg
      421 cgagcgaggc cctgttccgc gcgatctgtt acgccttgat tgcgttcgcg gtggtcttcg
      481 gtccgccggt tctcgacggt gtgcttcgcg cgggctaggc ttgcagcaaa cgaaaatccg
      541 aggggaggat gtgatggtcg atcgccgcga tgtattgaaa ctggggcttt ccgccgccgc
      601 ggcgctgact gcgccggcgg cgtttgcgca agccgcaaag ccgcgcaccc gcatcgtctt
      661 cctgggaacg aaaggtgggc cgcgtgtcgg actcgggcgc tcgaatcccg caaatctcgt
      721 ggtcgtgaac gacacgcctt tcgtgatcga ttgtgggatg ggcgtcagtc gccagcttgt
      781 cgccgcgggc gtgccgctgg aaacggtgag atacattttc atcagccatc accattccga
      841 tcacaatctc gaatacggca atctcgtcta taatgcctgg gccacgggac tttcgacgcc
      901 gatccattcg ttcggcccga agggcatcga ggcgatgacg aaaacgtttt tcgaactgaa
      961 caggttcgat atcgaaaccc gcatcgagga cgaaggccgt cccgacctgc gcaagcttct
     1021 gatcgccaaa gacatcaccg aagatggcgt ggtgctgcag acggctgacg tgaaagtcac
     1081 cgcgtttcgc acgccgcatc cgccgataac ggataacttc gcttacaagt tcgagacgcc
     1141 ggacggcgtc gtcgtgttct ccagcgatac caattacaat ccgcagctcg ccgaatttgc
     1201 caagcgcgcc gacgtgctcg tgcacgaggc gctgtatctg ccctgggtcg atcgcctcgt
     1261 cacccgggtc aagaacggtg cgacgctgaa gaagcatctt cttgaaagcc ataccgcggc
     1321 cgaagatgtc ggccgcatcg ccgacgccgc caacgtcaag gtgctggtga tgagccacct
     1381 ggtgcccgac gacccggacg tcacggatga cgactggcgc agcgaggcag ccaaaaactt
     1441 caagggccgg atcgtcgtcg caagagacct gatggagctg aggctgcctc tctgacgcga
     1501 tggatctgcg tcaggatcag ttgatctgcg gcgcgccccg gcgctgccgc cttcgcggct
     1561 ccggcgcacc cgccaagggc tcggtgtcct tcagcgcacc gatccggcgc agcgcagcct
     1621 ccgcggcgcg ctcgccgctc tcccaggcgc cgtcgacggt gccccacagc gtctcatggg
     1681 tcgcttcgcc ggcgaggaat agcgatccga tcggctcgag cagggccttg cgcgatgatt
     1741 gtgcgcccgg ggctgccgcc gagatcgcgc ccagcacgta cggtgatgca ttccatcggg
     1801 tggcgccgga tttcttcacc gcggccgccg cgtcgctgcc gaacagcttg gtcagccatt
     1861 ccagcgcgaa cgcgaccatg gctgcctccc cctccgccga gagatcgcgc ccgaaggaac
     1921 cggcaacatc gaccgagcac aacgacgatc cgccgatatt ggcataaagc agcgcggtcc
     1981 gcgccgacgt gctctgctcg ataatcacgt cgtcgcgcga cagcccgagc ggattgcggg
     2041 gcatcacgag cgcgatgtgg tcgtagctgc cgagagtgag ctttgctgcg gcgtccagcg
     2101 ctcgtttggg aatgtcgggg ttgaacttga tgttgcccgc agccaggaca ttggtcgaca
     2161 ccgtgatgac cgcggcgcgt gcggtaattt tgccggcggg tgtttcgacc ccgacgtcgc
     2221 gggttgacca gacgatccgg tttacgggtg tcgacagcgt gaccggcacc tgctcggaaa
     2281 gcttcgcgat cagcgtcccc agcccctggc ggcacgcgaa taccgagccg cgatcgccgg
     2341 cacgcgcctt gtcgaccgtg gagagttcct tcaggtcctt gcccgcgaaa ctggcgccga
     2401 gcgtgaactc gattgcgccc gcccagtcgc cgatatcctt cggcaatgcg gccgcacagg
     2461 acccgtcgag cctgcgcgag gcgtcgtcga tagcgcggtt ggcgcggacc agggtcgcca
     2521 aaaaatcctc ggtctcgccg gcgcgtgcgt tgcggcggcc gatccggatt ttctgtccgg
     2581 gcgccgtcac gatctcaaac ccggctcccc gcgcgagcct gatcatcggg ttggaatccg
     2641 gattatgcat ccagcgcgcg ccgcgatcga acggcacctc gaagctcgat gtatcggtga
     2701 tgcatcggcc cccgacgcga tctgccgcct ccaccacgat caccttgcgg ttggcagcca
     2761 tgatgcgccg cgctgcggca attccggcgg cgcccgcgcc gatcaccaca atgtcggcat
     2821 cgcgcggcag ggccgcgctg aaggcgcccc ccgaccaggc cggagcggat gccagtgccg
     2881 cggacgccga aagaaagccg cggcgcgtca ttgtcatggc atggtttccg aaatctttg
//
LOCUS       DATLJT010001527         1713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4050254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001527 DATLJT010000000
VERSION     DATLJT010001527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..186)
                     /locus_tag="VM910_34955"
     CDS             complement(25..186)
                     /locus_tag="VM910_34955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67746.1"
                     /translation="MTRPEGTTGFGGRSAPVASLRLIDAVAASLAFGALSLCVAVAFT
                     VLSIKSGLA"
     gene            complement(325..975)
                     /locus_tag="VM910_34960"
     CDS             complement(325..975)
                     /locus_tag="VM910_34960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI67747.1"
                     /translation="MYKLYSMQRSGNSYKVRLALALLNAPYQAIEVDILRGESRTPEF
                     LAKNPSGQVPLLEVEEGRYLAESNAILWYVCGGTPLAPESRVERAEALQWMFFEQHAL
                     EPNIGAAYFWLALVKGGRDLQTHALEDWMERGYGALKVMENHLKTHPYFAAKQLTVAD
                     IALYGYTHVANRCDFDLATFPSIRTWLRRVEQTPGFVTMDWRPDEVDDSASIAAGA"
     gene            complement(1160..>1713)
                     /locus_tag="VM910_34965"
     CDS             complement(1160..>1713)
                     /locus_tag="VM910_34965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HVI67748.1"
                     /translation="AISFLTLGSGVWHAFADSTNFTQTIVLEQGNTGWEKIQSVFSAA
                     RNWGADIKTAYAIQGTLALMLASSIAWLWQSDAAFELKASALATASLLATPYVLDYDL
                     VVLAVAIAFLARHGFSRGFRDYEVSLLAANWIVPLLARGVAGISGFPLGLVVMLALYG
                     FTLHCAMKERANAASSPARIAQA"
BASE COUNT          346 a          521 c          545 g          301 t
ORIGIN      
        1 gacgcgggga tgaaccgctg tcgctcaggc aagcccggac ttgatcgaca gcaccgtgaa
       61 ggcgacagcg acacaaagcg agagcgcgcc gaacgcgagc gaggccgcaa cggcgtcgat
      121 cagtctgagc gaggcaacgg gggcgcttcg gccgccaaag cccgtggtgc cttctggccg
      181 tgtcatggtc ggaagtccct ggagcgcgtg tgcgaatcac ccgcgccggc gaacgattgc
      241 aatatgggcc ggcaatattg gggcgggtca tcccgcggat ttgccgcaag cgttaacggt
      301 tgttaacgaa tgcggtctcg gccatcacgc gccggcggcg atgcttgcgg aatcgtcgac
      361 ctcgtccggc cgccagtcca tcgtcacgaa gcccggcgtt tgctcgacgc gcctgagcca
      421 ggtgcggatc gacgggaatg tcgccaggtc gaagtcgcag cggttggcaa catgggtgta
      481 gccgtacagc gcgatgtcgg cgacggtgag ctgtttggcg gcaaaatacg gatgcgtctt
      541 gaggtgattt tccatcacct tcagcgcgcc ataaccgcgc tccatccagt cctcgagcgc
      601 atgcgtctgc agatcccggc cgcccttgac cagcgccagc cagaaatacg cagcgccgat
      661 attgggctcc agcgcatgct gctcgaagaa catccactgc agcgcttcgg cgcgctcgac
      721 gcgcgattcc ggtgcaagcg gcgttccgcc gcaaacgtac cagaggatag cgttggactc
      781 ggcgaggtag cgtccctctt cgacttcgag caacggcacc tgcccgctcg gatttttcgc
      841 aagaaactcg ggtgtgcggc tctcaccgcg caaaatatcc acctcgatcg cttggtaggg
      901 cgcgttcaac agcgcgagcg caaggcgaac cttgtagctg ttgcctgagc gctgcatcga
      961 ataaagtttg tacattcgcc tgatcgccga atcgtcgaaa cgcgcttgtc cgatgggatt
     1021 gccatgttgc cccacaacgc ggctaaacaa tgatgctgaa gtgcgtggac cgcaagtatc
     1081 gtgacgtgga aaaggttgaa aaccgctacc gcactgcagc gccgtagaac ttccttccaa
     1141 cacgctttca caattcggtt cacgcttgcg cgatgcgtgc gggagaggag gccgcgttcg
     1201 cgcgctcttt catggcgcag tgcagcgtga agccataaag cgcgagcatc acaacgagcc
     1261 ccaatggaaa ccccgagatt ccggcgacgc cgcgcgcaag cagcggcacg atccagttcg
     1321 ccgcaagcag gctgacttcg tagtcgcgga aaccgcggga aaaaccgtgc cgcgcgagaa
     1381 aggcgattgc gacggcgagc acgacgaggt cgtaatcgag cacgtagggc gtggcgagca
     1441 ggcttgcggt cgcaagagct gaagccttca gctcgaacgc tgcgtcgctc tgccacagcc
     1501 aggcgatgct cgaagccagc atcagcgcga gcgtgccctg gatagcgtag gcggttttga
     1561 tgtcggcgcc ccaattgcgc gccgccgaga agacggactg aatcttctcc cagcccgtgt
     1621 tgccctgttc cagcactatc gtttgcgtga agttcgtgga gtccgcgaag gcatgccaga
     1681 cgccgctgcc gagcgtcagg aagctgattg cga
//
LOCUS       DATLJT010001528         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3591314,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001528 DATLJT010000000
VERSION     DATLJT010001528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3591314"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VM910_34970"
     CDS             complement(<1..191)
                     /locus_tag="VM910_34970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67749.1"
                     /translation="MPFEHVGVADHDRIAGVAHGGIERSLEADLRSDARRIARGNGNS
                     RLVAHAWTPVLYIGGIERMR"
     gene            159..1058
                     /locus_tag="VM910_34975"
     CDS             159..1058
                     /locus_tag="VM910_34975"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVI67750.1"
                     /translation="MVGDANVLERHAAACAIAAELCVIARAADADWSNRRVNVLGCAQ
                     PDLAALDFGTSGAASGRAALAFAGAAIKLALAGEVDAVVAAPQNETSIALAGIAFDGY
                     PSFVARATGADESDVYLMLCFDDVKIVHATLHRSMRQAIEMITRDNIARVIAATDRAL
                     KRLGTAAPKLAVGGLNPHAGEGGLFGREEIEVIKPAIEAAVAQGIAVTGPFGADTMFH
                     RRGVDAFIVMLHDQGHIAAKLMARNAVAALTIGSPILFSSVAHGSAHDIAGKGAADPR
                     AMIEAVLRLSKANQRLGLQPAPA"
     gene            1093..1401
                     /locus_tag="VM910_34980"
     CDS             1093..1401
                     /locus_tag="VM910_34980"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HVI67751.1"
                     /translation="MPTKIELCNASDVDVGAVLKVEKDDLTLAVFNLDGEFFVTDDHC
                     THGPGSLSEGFVEGDVVECNFHGGQFNIKTGAIVGPPCMVPIKTYPTVVEDGKVFIEV
                     "
     gene            1540..1773
                     /locus_tag="VM910_34985"
     CDS             1540..1773
                     /locus_tag="VM910_34985"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HVI67752.1"
                     /translation="MATQLMIYETAVPVSHARHGTWSLEVGTDYAFCRNINSVPLMAA
                     EFASAALEYAVVFGGTGDAVMPVTVLGVGRSCR"
     gene            1754..2308
                     /locus_tag="VM910_34990"
     CDS             1754..2308
                     /locus_tag="VM910_34990"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HVI67753.1"
                     /translation="MGVRADENLYVTKEGGWQAKYIPAFVRRYPFVFFSRDDSKTFTL
                     CIDEAFPGFNRSGRGERLFGDDGKPTPYVENVLKFLGQYQREFQRTQAFCKKLKDLNL
                     LEPMQAQIKLGSGERMALGGFSVVDRARLNTLSGNVLAQLVQSDELELIYAHLVSMRN
                     FATLRDRLAGAQPAHSAGDGPLPH"
     gene            2863..>3301
                     /locus_tag="VM910_34995"
     CDS             2863..>3301
                     /locus_tag="VM910_34995"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI67754.1"
                     /translation="MVLLDVGTPGGLAALLALRQALPDLKIVAIAVSDIEQVVVACAE
                     AGVSGFVSRDGSIQDLLTAIHCAMRNELVCSPRIAGMLFRRVAAIGSERSHAPDNSAL
                     TRREHEIVSLMTQGLSNKEIARQLRIQNATVKNHIHSILAKLHV"
BASE COUNT          647 a         1019 c         1013 g          622 t
ORIGIN      
        1 cgcattcgct cgatgccacc gatatagagg acgggtgtcc acgcatgcgc gacaagacgc
       61 gaattgccat tgccgcgggc gatccggcgg gcatcggacc ggaggtcagc ctcaaggctg
      121 cgctcgatcc cgccgtgcgc aacgcctgca atccgatcgt ggtcggcgac gccaacgtgc
      181 tcgaacggca tgccgccgcc tgcgccatcg ccgccgagct gtgcgtaatc gcgcgcgccg
      241 ccgatgccga ctggtccaac cgccgcgtca acgtcctcgg ctgcgcgcag ccggacctcg
      301 ccgcgctcga cttcggtacc agcggcgccg cgagcggccg cgccgcgctc gccttcgccg
      361 gcgcggcgat caagctcgcg ctcgcgggcg aggtggatgc ggtcgtcgcc gcgccgcaga
      421 acgagacatc gatcgcgctc gccggcatcg cgttcgacgg ctacccgtcg ttcgtcgcgc
      481 gcgcgaccgg cgccgacgag agcgacgtct acctgatgct gtgcttcgat gacgtgaaga
      541 tcgtccacgc cacgttgcac cgcagcatgc gccaggcaat cgagatgatc acgcgcgaca
      601 atatcgcgcg cgtgatagcg gccaccgacc gcgcgctcaa gcgcctggga acggcggcgc
      661 cgaagctcgc ggtcggcggc ctcaatccgc acgcgggtga agggggtttg ttcgggcgcg
      721 aggagatcga ggtcatcaag ccggcgatcg aggcggcggt cgcccaaggc atcgcggtca
      781 ctggcccgtt cggcgctgac accatgtttc acaggcgagg cgtcgacgcc ttcatcgtca
      841 tgctgcacga tcagggacac atcgccgcga agcttatggc gcgcaatgcc gtcgcggcgc
      901 ttacgatcgg cagcccgatc ctgttctcct cggtcgcgca cggcagcgcc catgatatcg
      961 ccggcaaggg cgcggccgat ccgagggcga tgatcgaagc ggtgctgcgg ctgtcgaagg
     1021 cgaaccaacg tctcggattg cagccagcgc cggcgtgaac gcatggttct gccaattcaa
     1081 tcaggatagt tgatgccgac caagatcgaa ctctgcaacg cttctgacgt ggacgttgga
     1141 gccgtgctca aggtcgagaa ggacgatctg acgctcgccg tcttcaacct cgacggcgag
     1201 ttcttcgtga ctgacgatca ctgcacgcac ggcccgggct cgttgtcgga aggcttcgtc
     1261 gaaggcgacg tggtcgaatg caattttcac ggcgggcaat tcaatatcaa gaccggcgcc
     1321 attgtcgggc cgccatgcat ggttccgatc aagacttatc ccacggtcgt cgaagacgga
     1381 aaggtcttca tcgaggtgtg agcaacgttt ttgcacatgg cctcgtgggg gcgacgcgcc
     1441 gacgtgtcgc ctctaggtgc gccatcgcgt gttagaagcg ttattatgaa caaaaaaagt
     1501 gttataattg acaaatttgt cgcgaaggaa aggcgagaca tggccacgca actgatgatc
     1561 tatgaaactg cggtgcccgt gtcccacgct cggcacggca cctggtctct tgaagtgggg
     1621 actgattatg cattctgccg aaacatcaat tcggtgccgc tcatggcggc ggagtttgcg
     1681 agcgcggctt tggaatacgc cgtcgtgttc gggggcactg gcgacgcggt gatgccggtc
     1741 accgtgctgg gcgttgggcg ttcgtgccga tgagaatctt tacgtcacca aggaaggcgg
     1801 ctggcaggct aagtacatac cggcgttcgt ccgccgctat cccttcgtct ttttcagccg
     1861 cgacgacagc aagacgttca cgctttgcat tgacgaggcc ttcccgggat ttaatcggag
     1921 cggccgcggc gagcgactgt ttggcgatga tggcaagccg actccctacg tggagaatgt
     1981 gctcaaattc ctggggcaat accaacgcga attccagcgc acccaggcat tttgcaaaaa
     2041 gctgaaggat ctgaacctgc tcgagccgat gcaggcgcag atcaagctcg gttccgggga
     2101 acgcatggcg ctaggcgggt tctcagtcgt ggaccgcgca cggctcaaca ccttgtcggg
     2161 caatgtgctc gcgcagttgg tgcagtcgga cgaactcgaa ctgatctatg ctcacctcgt
     2221 atcgatgcga aacttcgcga cgctgagaga tcgactcgcc ggggcgcaac cagcgcatag
     2281 cgcgggcgac ggcccgctac cccattagcg cggccagccc tggaactgct caggtgttgg
     2341 cataccaatc gcagggtgtg gccgaaggtc cgaacctttt cagctcgtga aatgctcacg
     2401 aaaaaatgcg caatcaacca tgtgaaggca gggagcatgc ttgtgtcaaa gagctatcct
     2461 cgaacctaac acaaaacgat aatgaaccgt tatgatactg ttacctcata ctgttacata
     2521 acggactctt cttgcggata ccgggaaaat atggcataat acaacccctc gttgaggcgg
     2581 cagtgtgatc attaagaact tcacacgtct gcctcgattc gagctgttca agttcacgag
     2641 ttccatcggg gtgggtcatg gaaacgcagg gcgcggatac aattgttggg tcaccaccgc
     2701 aaactctacc aagatcaaat ctccagcatc cgggaccgac ggtcctcatt gtcagcgacc
     2761 tgcgcctgct acgcgacggg ctcgtcctgt cgctttccca gcagccctct ctggccgtcg
     2821 cggactcgac ggacctgaca atatcgccct gcccgtcccg atgtggtgct gctcgacgtc
     2881 gggacgccag gcggtttggc ggcactgctc gcccttcgcc aggcactgcc cgacctgaag
     2941 atcgtcgcca ttgcggtctc cgacatcgag caggtggtcg tcgcctgtgc cgaagccggg
     3001 gtatcaggat tcgtctcccg cgacggctcg attcaagatt tgctgacggc gatacactgc
     3061 gccatgcgca acgagctggt ttgttcgccg cgcatcgccg gtatgctgtt tagacgcgtc
     3121 gccgccatcg gctccgaacg aagccatgcc ccagacaaca gcgcgctgac gcggcgcgag
     3181 cacgagatcg tctcgctgat gacgcaaggc ctttccaaca aggagatcgc gcgccagctg
     3241 cgcatccaga acgcaacggt caagaatcat attcacagca tcctcgccaa gctgcatgtg
     3301 c
//
LOCUS       DATLJT010001529         1568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2441008,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001529 DATLJT010000000
VERSION     DATLJT010001529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441008"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VM910_35000"
     CDS             complement(<1..738)
                     /locus_tag="VM910_35000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI67755.1"
                     /translation="MPNSSAAVAQYLPFLRRYARALSGNQTSGDAYVAATLEALIADR
                     AVIEDPRGPRVALYHLFTKIWNSVSVNGAATPGEGVLPGEQKLANITPLPRQAFLLVA
                     LEGFSEPDAARVLDIDVMKLRGLVEESGRELAAEIATDVLIIEDDTFIAMELETLVEG
                     LGHRVLGVARTHAEALALTKTKRPGLILADIQLADGSSGLEAVNELLASFEAPVIFIT
                     AYPERFLTGERPEPAFLIAKPFQPATVS"
     gene            892..1239
                     /locus_tag="VM910_35005"
     CDS             892..1239
                     /locus_tag="VM910_35005"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HVI67756.1"
                     /translation="MNERKPVRAAIAGEKAAPPDAMQHQPPRSEATMHTEAQVRKAGG
                     RLSREDQRRLGDILQRVYDEVLRQGVPDRFKTLLDQFDRDVSAAETMRGQAGEDDDHL
                     VRAKAFDNKGSRS"
     gene            1236..>1568
                     /locus_tag="VM910_35010"
     CDS             1236..>1568
                     /locus_tag="VM910_35010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVI67757.1"
                     /translation="MTMRSSVRDAMLAAVPSLRAFAISLCGNVDRADDLVQETLLRAM
                     ANIDSFQPGTNMSAWLFTILRNLFRSEYRKRRREVEDTDGSYAESLKSHPEQHSRVEF
                     EEFRVALGK"
BASE COUNT          322 a          468 c          515 g          263 t
ORIGIN      
        1 cgagaccgtc gccggctgga acggcttggc gatcaggaac gccggctcgg gccgctcacc
       61 ggtcaggaag cgctcgggat aggcggtgat gaagatcacc ggcgcttcga aagatgcgag
      121 caactcgttg accgcctcga gtcccgaact gccatcggcg agctggatgt ccgcgaggat
      181 cagccccggt cgcttggtct tggtcagcgc gagagcctcc gcgtgagtgc gcgcaacacc
      241 gagcacccgg tgaccgaggc cttccaccag ggtttcgagc tccatcgcga tgaaagtgtc
      301 atcctcgatg atcagaacgt cggttgcgat ctcggccgcg agctcgcgac cggattcttc
      361 gaccagtccg cgcagcttca tgacgtcgat gtcgagcacg cgcgccgcat ccggctccga
      421 gaagccttcg agcgcgacca gcagaaatgc ctggcggggc aggggagtga tgttggcgag
      481 cttctgctct ccggggagca cgccttcgcc gggagtggcg gcgccattga ccgacaccga
      541 gttccagatt ttcgtgaata ggtgatagag ggcaacccgc ggccccctgg ggtcttcgat
      601 cacggcgcgg tccgcgatca aggcttccag cgtggcagcg acgtaggcgt ccccggacgt
      661 ttggtttccg ctcaaggctc tcgcatagcg tcgcagaaat ggcaggtatt gggctaccgc
      721 agcggacgaa ttgggcacgg aaccctcccg ttcaattgct aagcttacgc ggcaaaaact
      781 ggcctgtggg aaaaaagttc catgacacgg aacgaaccgt tctgcgtcac gttatgctga
      841 gataaaagca ctgaggggcc cgggcggggg catttcgccc gggaacaaca aatgaacgag
      901 cgcaagccgg ttcgggcggc catcgcgggg gaaaaggctg cgccgccgga cgcgatgcaa
      961 caccagcccc cgaggtcaga ggcgaccatg cataccgagg cgcaagtgag aaaggctggg
     1021 gggcgcctca gccgcgagga tcaacgaagg ctgggcgaca tccttcagcg cgtctacgac
     1081 gaggtgttgc gccaaggcgt ccccgatcgg ttcaagaccc tgctggacca gttcgacaga
     1141 gacgtcagcg ccgccgagac catgcgcggg caagccggag aggacgacga tcatctcgtg
     1201 agagccaagg cattcgacaa caaggggtcg agatcatgac gatgcgatca tccgtacgtg
     1261 acgcgatgct cgcagcggtg ccgagcttgc gcgcgtttgc catttcgctc tgcggtaacg
     1321 tggatcgcgc cgacgatctg gtgcaggaaa cgctgctgcg tgccatggcg aatatcgatt
     1381 cgttccaacc tggaacgaac atgtcggcgt ggctgttcac cattctccgc aacctgttcc
     1441 gctccgaata tcgcaagcgc cgtcgcgaag tcgaggacac cgacggaagc tatgcggaga
     1501 gcctgaaatc gcatcccgaa cagcatagtc gcgtggaatt cgaggaattc cgcgtggcgc
     1561 tcggcaag
//
LOCUS       DATLJT010001530         4090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1753474,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001530 DATLJT010000000
VERSION     DATLJT010001530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753474"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1579
                     /locus_tag="VM910_35015"
     CDS             344..1579
                     /locus_tag="VM910_35015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI67758.1"
                     /translation="MSGNARELAASFDLEKLTPEFYVNPYPTYRALRENEPIKRMPNG
                     SYFLTRYDDLVTAYKNTKAFSSDKKKEFAPKYGASLLYEHHTTSLVFNDPPIHTRVRR
                     LIMGALSPRAIAEMEPNLIRLVDGLLDRIAAKDRFDLIGDYAAAIPVEVIGNLLDVPH
                     EEREPLRDWSLAILGALEPVIGEAAFARGNKAIKDFLAYLEVLVERRRAKPGNPNQDV
                     LTRLIQGEENGERLSAKELLHNCIFLLNAGHETTTNLIGNGLVALTEHPAEKRRLIEH
                     PDMIKTAVEEMLRYESSNQLGNRMTVEEVQLGGLTLPAGTPITLCIGAANRDPAQFPD
                     PERFDIGRTPNRHLAFATGAHQCAGMALARLEGAIAISRFLARFPHYALAGEPVRGGR
                     VRFRGFLSVPCVTTGPGSV"
     gene            complement(1633..2496)
                     /locus_tag="VM910_35020"
     CDS             complement(1633..2496)
                     /locus_tag="VM910_35020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HVI67759.1"
                     /translation="MRDFSADHRWLSLNTATVREQGDLLQIIEACARHGIRAIDPWRD
                     QVAAVGLERAARAVRDAGLELSGYCRGGMFTSDAGRRNEVRDDNRRAVDEAKALRAPC
                     VVLVVGGLPQYSRAGSAASRDIAAARAQVHDAIAEMLDYARQANMPLAIEPLHPAYAA
                     DRACVNTTKQALDLCDALDPARTGTLGVALDVYHIWWDPELMAQIGRAGKDRLLAFHV
                     CDWLVPTKDILNDRGMMGDGVIDIKSVRAALEAQGYAGYSEIEIFSNHWWARPMDEVL
                     RTCIARHRSVV"
     gene            complement(2496..3689)
                     /locus_tag="VM910_35025"
     CDS             complement(2496..3689)
                     /locus_tag="VM910_35025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVI67760.1"
                     /translation="MNKPVMPVSSYQLKLPKADGSLETYRLAASRSFPARLEGTLNRV
                     AFSAAHVVANPLADVDPWLAAAVDWDRTIAFREHVWDLGLGVAEAMDTAQRGMGLDWP
                     TSLELITRSVRAAKARGNALVFSGAGTDHLAPDDARTIDDVIRAYEEQIADIEKVGGR
                     IILMASRALARVGRNAEDYAKVYDKVLSQVREPVIIHWLGDMFDPALSGYWGTADLDK
                     AMDTALGIINANAAKVDGVKVSLLDKQREIDMRRRLDARVRMYTGDDFNYAELIAGDG
                     QGFSHALLGIFDAIAPAASYALSRLAAGDLAGFHDVLGPTVPLSRHIFRAPTRFYKTG
                     VVFMAYLNGHQDHFTMIGGQESARSTVHLAELFRLADQAGLLADPEIATRRMTSVLSV
                     RGVEP"
     gene            complement(3703..>4090)
                     /locus_tag="VM910_35030"
     CDS             complement(3703..>4090)
                     /locus_tag="VM910_35030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVI67761.1"
                     /translation="VIAHINMSWVTRVYRDDLVTFQVDGTHGSAVAGLTDCVIQARQA
                     TPRPVWNPDEKRLHDFYGDWQKLPDNVACDNGFKEQWELFIRHVCEGAPYKYTLLEGA
                     KGVQLAECALQSWRERRWIDVAPIKL"
BASE COUNT          769 a         1334 c         1276 g          711 t
ORIGIN      
        1 ggttcgccga gcacgcgatt gatcaattgc gaatggccgg catccggaac gtaaggcacc
       61 tggcggactt cgaaacgctt gagaagcgca aagatgtcgt cgggccagga ggccgcttgt
      121 tcggcgggag tatcggagcg cgcgtgcata tgtccatcgg tcgtttggaa cgcagaatgc
      181 ggccgaactg tagacggtgc cgcagtctcc atcaaagcgc aaaaaccgca tacagccctg
      241 cgagaaggat cgcctttacc ggaacacgct tcggaattgg gttgcgcgca accggcagcg
      301 ggcttacgat agttgcaggt gagactaatt tgagactggc gggatgagtg gaaacgcacg
      361 agagctggcg gcgagcttcg acctggagaa actgacgccg gaattctacg tcaatcccta
      421 cccgacctat cgtgcgctcc gggaaaacga gccgatcaag cggatgccga acggttcata
      481 ctttctcacc cgctacgacg acctggtcac cgcctacaag aacaccaagg cgttctcgtc
      541 ggacaagaag aaggaatttg ctccgaaata cggcgcgtcc ctgctctacg agcaccacac
      601 cacgagcctt gtcttcaacg acccgccgat ccacacccgc gtgcggcggc tgatcatggg
      661 cgcgctgtcg ccgcgcgcca ttgcggagat ggagcccaac ctgatccggc tcgtcgacgg
      721 gctgctcgat cgcatcgccg ccaaagaccg cttcgatctg atcggcgatt atgccgccgc
      781 gatcccggtc gaggtgatcg gcaatctgct cgatgtgccg catgaggagc gcgaaccgtt
      841 gcgcgactgg tcgctcgcga ttttgggcgc gctggaaccg gtgatcgggg aagctgcgtt
      901 cgcgcgcggc aacaaggcga tcaaggactt tcttgcttac cttgaagtct tggttgagcg
      961 ccggcgcgcc aaaccgggca acccgaacca ggacgtgttg acgcggctga ttcagggtga
     1021 agagaacggc gagcggttgt cggccaaaga actactgcac aactgcatct tcctgctcaa
     1081 cgccgggcat gagaccacca ccaatctgat cggcaacggt ctcgtcgcgc tcacggagca
     1141 tccggcagag aagcggcggc tgatcgaaca ccccgatatg atcaagaccg cggtcgagga
     1201 aatgctgcgc tacgaaagct cgaaccaact cggcaaccgc atgacggtgg aagaggtgca
     1261 actcggcggc ctgacgctgc cggcggggac gccgattacg ctctgcatcg gggcggccaa
     1321 ccgcgaccct gcgcaatttc ccgaccccga acgcttcgac atcggcagaa cgccgaaccg
     1381 gcatctggcc tttgccacgg gcgcgcacca atgcgccggc atggcgctgg cgcgactcga
     1441 aggcgcgatt gcgatctcgc gctttctggc gcgctttcca cattacgcgc tcgcgggcga
     1501 accggtgcgt ggcgggcgcg tgcgcttccg cggattcttg agcgtgcctt gcgtgaccac
     1561 aggccccggt tccgtttagg ctgaattttc gttgcccgtc gcaacgagtg cagccgccgt
     1621 tgcagcgacc gatcaaacca ccgaccgatg ccgcgcaata caggtgcgca atacctcgtc
     1681 catcggcctt gcccaccagt ggttggaaaa aatctcgatc tcggaatagc cggcgtaacc
     1741 ctgggcttcg agcgccgcgc gcaccgattt gatatcgatc acgccgtcgc ccatcatgcc
     1801 gcggtcgttg agaatgtcct ttgtcggcac cagccagtcg cagacatgaa acgccagcag
     1861 acgatctttc ccggcgcggc cgatctgggc catcaattcc gggtcccacc agatgtgata
     1921 gacatcgagc gcgacgccca gagtgccggt ccgtgccggg tccagcgcgt cgcagagatc
     1981 gagcgcctgt tttgtggtgt tcacgcaggc acgatcggcc gcataggccg gatgcagcgg
     2041 ctcgattgcg agcggcatgt ttgcctgcct cgcatagtcg agcatctcgg cgatggcgtc
     2101 gtgcacctgc gcccgcgccg ccgcgatatc cctggaggct gcgctgccgg cccgcgaata
     2161 ttgcggtaga ccgccgacga cgagcacgac gcagggcgcg cgcagcgcct tcgcctcatc
     2221 gaccgcacgg cgattgtcgt cgcgcacctc gttccggcgc ccggcatccg acgtgaacat
     2281 gccgccgcgg caatagccgg acagttcgag gcctgcatcg cgcaccgcac gcgcggcgcg
     2341 ctccagccca acggctgcga cctgatcccg ccagggatcg atggcgcgga tgccgtgccg
     2401 cgcgcaagcc tcgatgatct gcaggagatc gccctgctcg cgcacggttg cggtgttgag
     2461 cgacagccag cggtgatcgg cagaaaaatc ccgcatcacg gctcgacacc gcgcacgctc
     2521 agcacggacg tcatccgtcg cgtggctatt tccggatcgg caagcagtcc ggcctggtcg
     2581 gcgagacgga acagttcggc aagatgcacc gtggagcgcg cactctcctg cccgccgatc
     2641 atggtgaaat gatcctgatg accgttgaga taggccatga acaccacgcc ggtcttgtag
     2701 aaccgcgtcg gcgccctgaa gatgtggcgc gacagcggca ccgtcggtcc cagcacgtcg
     2761 tggaagcctg ccaggtcgcc cgcggcaagc cgcgacagcg cgtaggatgc ggccggcgcg
     2821 atcgcatcga aaatgccgag caaggcatgg gagaagccct gcccgtcgcc cgcgatcagt
     2881 tctgcatagt tgaaatcgtc gccggtatac atcctcacgc gcgcatcgag ccgccggcgc
     2941 atgtcgatct cgcgctgctt gtcgagcagc gacaccttga cgccatcgac cttggcagcg
     3001 ttcgcgttga tgatgcctaa agccgtatcc atcgccttat caaggtccgc cgtgccccag
     3061 tagcctgata gcgcggggtc gaacatgtcc ccgagccaat gaatgatcac gggctcgcgg
     3121 acctgcgaca acaccttgtc gtaaaccttc gcgtagtcct cggcgttgcg gccgacccta
     3181 gccagcgcgc gcgacgccat caggatgatc cgcccgccga ccttctcgat atcggcaatc
     3241 tgctcctcat aggcccggat gacgtcgtcg atcgtcctgg catcgtcggg cgcgagatga
     3301 tcggtgccgg cgccggaaaa caccagtgca ttgccgcgcg ctttcgccgc ccgcacggag
     3361 cgcgtgatca actcgagcga ggtcggccag tcgagcccca tgccgcgctg cgcggtgtcc
     3421 atggcttctg cgacgccgag gccgaggtcc cagacatgct cgcggaacgc gatcgtcctg
     3481 tcccaatcga ccgcggcggc gagccacgga tcgacgtcgg cgagagggtt cgcaaccacg
     3541 tgggctgcag agaacgccac ccggttcaac gtgccttcca gtcgtgccgg aaagctgcga
     3601 gaggccgcga gccgataggt ttcgagcgag ccgtccgcct tcggcagctt cagttgatat
     3661 gacgaaacag gcatgaccgg tttgttcatg gccgctctct cctcacagct tgatcggcgc
     3721 cacgtcgatc cagcggcgct cgcgccagct ttgcagcgcg cattcggcaa gctgcacgcc
     3781 ctttgcgcct tccagcagcg tgtatttgta gggcgcgcct tcgcagacgt ggcgaatgaa
     3841 cagctcccat tgctccttga agccgttgtc gcaggcgaca ttgtccggca gcttctgcca
     3901 gtcgccataa aaatcgtgca gccgcttctc gtcgggattc cagaccggtc gcggcgtcgc
     3961 ctggcgggcc tggatcacgc aatcggtgag gccggccacc gccgagccgt gagtgccgtc
     4021 gacctggaag gtgacgagat cgtcgcggta gacccgcgtc acccagctca tgttgatatg
     4081 cgcgatcacg
//
LOCUS       DATLJT010001531         6546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_373135,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001531 DATLJT010000000
VERSION     DATLJT010001531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373135"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..233
                     /locus_tag="VM910_35035"
                     /pseudo
     CDS             150..233
                     /locus_tag="VM910_35035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493892.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
     gene            complement(234..662)
                     /locus_tag="VM910_35040"
     CDS             complement(234..662)
                     /locus_tag="VM910_35040"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI67762.1"
                     /translation="MLELYHSINSVCAQKVRIALEEKGQQAKDHIMTLRGDQYEPAYL
                     KLNPNSVVPTLVHNGEPIVESSLILYYIDDAFPEPSLMPKEPYQCHRVRMYNKLIDEY
                     VHNSCTILTFATAFRPAFLKMAREAWRAEINKTPLKRRAE"
     gene            complement(1162..1305)
                     /locus_tag="VM910_35045"
     CDS             complement(1162..1305)
                     /locus_tag="VM910_35045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67763.1"
                     /translation="MAHAVPLEAVQIEMQSNPSRMLVRALAGGHFAVEDNLDYTASNM
                     ERF"
     gene            complement(1407..1841)
                     /locus_tag="VM910_35050"
     CDS             complement(1407..1841)
                     /locus_tag="VM910_35050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67764.1"
                     /translation="MAGATLVGVSVSAAGECPAGKSKADVRMPVAHAGKGVTDTVLAA
                     IDLEKEPANIKERQLRFRKLTIEPGGIVPWHSHADRPAIIYIAEGEIVEYASNCADPI
                     VHQAGDIRPETSGTAHWWQNLGSKTVILFVGDVLHDKTDHNM"
     gene            2248..2955
                     /locus_tag="VM910_35055"
     CDS             2248..2955
                     /locus_tag="VM910_35055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HVI67765.1"
                     /translation="MTSKPLPDALEEAFRYCRDMEASLGERLDSFARAVRALSPPFAD
                     AVDHLVTRLQKSGVGQNAPQVGEPMPPFHLPDEAGRVVSLDRLLSEGPIAVTFHRGHW
                     CPYCRLNTKALAQAQTEVERNGGQIVAIMPDRQQFAEKFKTESQARFPILTDMDNGYA
                     LSLNLVIWVGAEMEQMIAAAGRDVPSYQGNTSWMLPIPATFVVGTDARVKARFVDPDY
                     RKRMGVEALLTALKSAR"
     gene            complement(2952..3839)
                     /locus_tag="VM910_35060"
     CDS             complement(2952..3839)
                     /locus_tag="VM910_35060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI67766.1"
                     /translation="MEMHQVRYFLATVSERNFTKAAETCNVTQPSLTRAIKQLEEELG
                     GDLFRRERPHAQLTELGQRMYPLLKQCYESALGARSLASAIKSGELGTLKLALSRTIN
                     LDLLTSHLAELDRLFNRLELKLLRGTAPEIVELLKAGDAELAIAADLGDAWERLDRWP
                     LFTEEFALILGAKHRLASRRAIEIDDLRQERWLRRAYCEHFEGFMSLIHGHSLDVDQG
                     HELTSERDLITLLESDFGIAFAPRSISCPDTLKQTAVNGLEFRRTIYLYGVAGRERTA
                     AASAILKMLRAANWSRYAS"
     gene            3947..4771
                     /locus_tag="VM910_35065"
     CDS             3947..4771
                     /locus_tag="VM910_35065"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HVI67767.1"
                     /translation="MNVAVKPEKINVEAPLAGKLSLVTGSTSGIGLGIARALAAAGSD
                     IVLNGFGKPDDIAQAQANIASDFKVRVAYFKADMSKPLAIREMIEGVLQSSGRLDILV
                     NNAGIQHVAPLQDFPPEKWDAILAINLSSAFHTTRLVLPAMLQNKWGRIINIASTHGL
                     VASPFKSAYVAAKHGIIGLTKVTALETAEDGITCNAICPGYVYTPLVEAQIEGQAKAH
                     GIPREQVIRDVLLAQQPNKRFAAVEELGALTVFLASAAAASITGVALPVDGGWTAH"
     gene            4825..5970
                     /locus_tag="VM910_35070"
     CDS             4825..5970
                     /locus_tag="VM910_35070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI67768.1"
                     /translation="MERGKERAERKPPQEQRQVVLVFQGGGALGAYQAGVYQALHESG
                     IEPDWIIGTSIGAINASLIAGNEPDNRVERLEEFWRRMRRKDFWGLANWPGLADTMSY
                     WSTLLGGIPGFFEPNPLAFLGAHYPLGRDKAGFYSTAPLQKTLTELIDFSLIPRCTPR
                     LTVGAAHVRTSEMRYFDSRETAITARHVMASGALPPAFPAVRIDGELYWDGGILSNTP
                     TEAIFDDNPRRNSLIFAVHMWNPMGPEPETIWEVLHRHKDIQYSSRVASHIARQRQVH
                     RLRHVISELVKLIPEGARKDPLVKDLAEYGCLTRMHVVRLLAPRLDNENHTKDIDFSP
                     TGIRLRWEAGYADTIRALRRSPWDDACDPLEGVILHEPLPDVAVAIE"
     gene            6086..>6546
                     /locus_tag="VM910_35075"
     CDS             6086..>6546
                     /locus_tag="VM910_35075"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HVI67769.1"
                     /translation="MSLGLRAAAVTDLFASLARLLSPVLDVLIRLWLAEGFLVADVMQ
                     HMLGDQTTERDHLPPSALLYASLAATGFGIFVQTICPVLLAAGLFTRLAALALLLQVL
                     VLQMPGHAQLAPYSVALLGWIVVLGPGPLSLDGMFRPGIGAAALPLVGLVSR"
BASE COUNT         1231 a         1984 c         2008 g         1323 t
ORIGIN      
        1 gtccagtctg cctccgacat gcggtcgaag atagctgcct tgaccgaggc ccgcgcgcgc
       61 atgcgcatcc accagtcagc gatggcgggg tagcgatccc acaatgcgct gagtttcagc
      121 agttccagcc gcaggatata agcacgctaa tgagcatcga aaggaaggtt gcggtcatca
      181 ccggtgcctc gcagggcatc ggtgcagcgc tgataaaggc ctatcgcgcc cgcttactcc
      241 gcgcggcgct tgagcggggt cttgttgatt tcggcccgcc aggcctcgcg cgccatcttc
      301 aggaaggctg ggcggaatgc ggtggcgaag gtcagaatag tgcacgagtt gtgcacatat
      361 tcgtcgatca gcttgttgta catgcgcacg cgatggcact ggtacggctc tttcggcatg
      421 agagacggct cgggaaaggc gtcgtcgatg taataaagga tcagcgatga ttcgacgatc
      481 ggctcgccgt tgtggacgag cgtgggcacc acgctgttcg gattgagttt caggtacgcg
      541 ggctcgtact ggtccccgcg cagcgtcatt atgtggtcct tggcctgctg tcccttttcc
      601 tccagcgcga tgcgcacctt ttgggcgcag accgaattga tgctgtgata gagctccagc
      661 acgtcatcct ccttaactaa atcgcaagcc gctcgatcgc acctatttgc atagcaaccg
      721 ccagacgatg tttcttactg acctgaacga gaatctccct ctggtgatat caatacctcg
      781 cttgatgaaa tcgcgcgaaa ttttgtcgac gatgtcctcg ggtcccgttt ccagaaggcc
      841 agtggctata acttccaatg ggtagattgc tcggctttcc ctcgacaacc ctagctgttc
      901 cccggcccac agccctacca acatgtcgcg tcggccgatc aagtgatctc gctcttaacg
      961 cacggcgaaa tcatgttgca ttgatccaac tttcgacgcg ccaagttcct tgcgcattgc
     1021 atttgattac atcgttgtca gaccaataga gcgcaggaac gagtcggcgt gaaataccaa
     1081 atcgacatag attatatagc ttccatctat ggatcgggtt ttcatttgat cttggtcaac
     1141 gaattgaagc ggtacttccg tctagaaacg ctccatattg cttgcggtgt agtcgagatt
     1201 gtcctcgact gcgaaatggc cgccggcgag ggcgcgcact agcatccggg aagggttgct
     1261 ctgcatctca atctgtaccg cttccaaagg tacagcgtgc gccatgttta gaagctcaat
     1321 tctggaacga gaggcgatgt gacgccccgc cggggcactc caagcttttg ggcgcacata
     1381 cggtttcaaa ggacgccggt gcgacatcac atgttgtggt cggtcttatc gtgcagtacg
     1441 tcgcccacaa acaagatgac cgtcttgctc ccgaggttct gccaccagtg cgcggtgccg
     1501 ctcgtctccg gcctgatgtc gccggcctgg tgcacgatcg gatcggcgca gttgctggcg
     1561 tattcgacga tctcgccctc agcgatgtag atgatcgccg gacggtcggc gtggctgtgc
     1621 cagggcacga tgccgcctgg ctcgatcgtg agcttgcgga atcgcagctg gcgctccttg
     1681 atgttggccg gctccttctc gaggtcgatg gcggcgagca ctgtgtcggt gacgcccttg
     1741 ccggcatggg cgaccggcat gcgcacgtcg gctttggact tgccggcggg acattcgccg
     1801 gcggcggata ccgacacgcc gacgagcgtc gcgcccgcca ttatcccggc gagggcaagg
     1861 cttcggccga acgcgcggcc acttgcaatg cgattgaaca tggtcgtggt ctcctctgtt
     1921 acgccccagc cgcagcgagg ccgtaacggt gcgcagcatt catcggacgg acggagaacg
     1981 gaaatgccga ttggctctgg actcgatagc ggactgctat gggaagaagg ctccagctga
     2041 tggagcgggt ctcgaattac ttcgccgtct ggacggcaaa gtgcggtttc ctcggatcgg
     2101 acaatagcgc ccaggcatgg gctcgatagc gattcgatat ttcccggcga acgctgcgta
     2161 tccaatgatc ccgtcggcga cgcggtggac gttctgataa gattacccca acgggttgtc
     2221 ggcgccccgc tcccctggag tctttgaatg acatcaaagc cattaccgga cgctctcgag
     2281 gaggctttcc gctattgccg cgatatggaa gcatctttgg gtgagcggct tgatagcttt
     2341 gcccgggcgg tgcgggcgtt gagcccgccc tttgccgatg ccgtggatca tctggtgacg
     2401 cggctgcaaa aaagcggggt cggtcagaat gcccctcagg tcggcgagcc gatgccgccg
     2461 tttcatctgc ccgacgaggc gggacgtgtc gtcagccttg accggttgtt gagcgaaggt
     2521 cctatcgcgg tgacgttcca tcgtgggcac tggtgtcctt actgccggct gaataccaag
     2581 gccttggcac aggcgcagac cgaggtcgaa cgaaacggtg ggcagatcgt cgcgatcatg
     2641 cccgaccggc agcagtttgc tgaaaaattc aaaaccgaat cgcaagcgcg gtttccaatc
     2701 ctgacggaca tggacaacgg ctacgcgctt tcgctcaatc tagtgatttg ggtcggggcg
     2761 gaaatggagc aaatgattgc agcggcagga cgggatgtcc cgagctacca aggcaacacc
     2821 tcttggatgc ttcccatccc tgcgaccttc gtggtcggca cggatgcaag ggtcaaggcg
     2881 cgctttgtcg atcccgacta ccgcaagcgc atgggggtcg aagccctgct caccgcattg
     2941 aaaagcgcac gctagctagc gtaacgcgac caattcgccg cccgcagcat tttcagaatg
     3001 gccgatgctg ccgcagttcg ttcacggccg gcgacgccgt aaagataaat cgtgcgacga
     3061 aactcgagac cattgaccgc ggtctgcttc aaagtgtcgg gacatgaaat gctccgcgga
     3121 gcaaacgcga tgccgaaatc cgattcgagc aacgtgatca aatcccgttc cgaggtcagc
     3181 tcgtggcctt ggtccacatc aagactatgg ccgtgaatga gtgacatgaa cccttcgaag
     3241 tgctcgcagt aggcgcgccg caaccagcgc tcttgacgca ggtcgtcaat ctcgatggcg
     3301 cggcgcgatg cgaggcgatg cttggcgccg aggatgagag cgaactcttc ggtgaacagc
     3361 ggccagcgat cgagacgctc ccaggcatcc cccaggtccg cggcgatggc gagttcggcg
     3421 tcgcccgctt tgagaagctc gacgatttcg ggcgccgtgc cgcgcaacaa tttcagctcg
     3481 aggcgattga acagccggtc gagctcggcc aggtgcgacg tcagcaggtc gagattgatc
     3541 gtgcgggaaa gggcaagctt aagggtgccg agctctccgc tcttgatggc ggaagccaac
     3601 gagcgcgcgc cgagcgcgct ctcgtagcac tgcttgagca gcggatacat gcgctggccg
     3661 agctcggtca gctgggcatg cggccgttcg cggcggaaga gatcgccgcc gagctcctct
     3721 tcgagctgct tgatggcacg agtcagcgac ggctgcgtca cgttgcacgt ctccgccgcc
     3781 ttggtgaaat tgcgctcgct gaccgtcgcc aggaaatatc tgacctgatg catttccata
     3841 gcggaattct atcaagctgt tagccaattg gcattggcgc tgcacggccc cggtccggca
     3901 gataggcacg aaagatcgag cgtcgctgca acggagtgca tgggccatga acgtcgccgt
     3961 caagccggaa aagatcaatg tcgaagcgcc ccttgcgggc aagctgtcgc tagtcaccgg
     4021 ctcgaccagc ggtatcgggc tgggcatcgc tcgggcgctc gccgccgccg gctccgacat
     4081 cgtgctcaac ggcttcggca agccggacga catcgcgcag gcgcaggcca acatcgcctc
     4141 ggatttcaag gtgcgcgtgg cctatttcaa ggccgatatg tcgaagccgc tcgctatccg
     4201 cgagatgatc gaaggagtcc tgcagtcgtc cggccggctt gacattctcg tcaataacgc
     4261 aggcatccaa cacgtggccc cattgcagga tttcccgccc gaaaagtggg atgccatcct
     4321 cgccatcaat ttgtcgtccg cgtttcacac cacacgactc gtcttgcccg cgatgcttca
     4381 gaacaaatgg ggtcgcatca tcaatatcgc ctcgacgcac ggtcttgtgg cctcgccgtt
     4441 caaatcggcc tacgtcgccg cgaagcacgg gatcatcggc ctgaccaagg tcacggcgct
     4501 cgagactgca gaagacggca tcacctgcaa cgccatctgt ccgggttacg tgtacacccc
     4561 gctggtcgag gctcagatcg aggggcaggc gaaggcgcac ggcatcccgc gggaacaggt
     4621 tatccgcgac gtgctactcg cgcagcagcc gaacaagcgt ttcgctgccg tggaagagct
     4681 gggcgcgctc accgtgtttc tcgcaagcgc ggccgcggcc tcgatcaccg gcgtcgcttt
     4741 gccggtcgat ggcggttgga ccgcgcacta accgagagcg cagcctggga gggaagcatg
     4801 ctggacgttc cgagtacgcg ctacatggag cgcggtaaag aacgggctga gaggaagcca
     4861 ccgcaagagc agcggcaggt tgttctcgtg tttcagggcg gcggcgcgct cggcgcctat
     4921 caggcgggcg tctaccaagc tttgcatgaa tcggggatcg agccggattg gatcatcggc
     4981 acctcgatcg gcgccatcaa cgccagcctg atcgccggca acgagcccga caatcgcgtg
     5041 gagcggctgg aggaattctg gcggcgcatg cggcgcaagg acttctgggg cctggcgaat
     5101 tggccggggc tcgccgacac gatgtcgtac tggtcgaccc tactcggcgg cattccggga
     5161 ttcttcgagc ccaatccgct ggcatttctc ggcgcgcact atccgctcgg acgcgacaag
     5221 gccggcttct actcgacggc gccgctgcag aagactctga ccgagcttat cgatttttcc
     5281 ctcattccgc gttgcacccc acggctcacc gtcggcgccg cgcatgtgcg cacgagcgag
     5341 atgcgctact tcgacagccg cgagaccgcg attacggccc ggcatgtcat ggcgtcgggc
     5401 gcgctgccgc cggcctttcc ggcggtccgc atcgatggcg aactctactg ggacggcggc
     5461 attctctcca atacgccgac cgaggcgatt ttcgacgaca acccgcgccg caattcgctg
     5521 atcttcgccg tgcatatgtg gaatcccatg ggaccggagc ccgagacgat ctgggaagtg
     5581 ctgcaccggc acaaggacat tcagtactcg agccgcgtcg cgagtcacat tgccagacaa
     5641 cgtcaggtgc acaggctgcg ccacgtcatc tccgaattgg tcaaattgat ccccgagggc
     5701 gcccgcaagg atccgctcgt gaaggatctg gccgagtacg gctgcttgac gcgcatgcat
     5761 gtggtgcgac tgctcgcacc tcgcttggac aacgaaaacc acaccaagga tatcgatttc
     5821 agcccgacgg gcattcgcct gcgctgggaa gccggttatg ccgacacgat ccgtgcgctg
     5881 cggcgctcgc cctgggacga tgcatgcgac ccgctcgaag gcgtcattct gcacgagccg
     5941 ctgcccgatg ttgcggttgc cattgaatga tgacttcccg aatctctcca tgtagtgcca
     6001 ttccatggtg aaatcccgaa cagcgcacgg tcccatgact gcgggcacga gtgagataca
     6061 ggccaggcac gagacgccgg tctttgtgtc tctcggactc agggccgctg ccgttaccga
     6121 cctgttcgca tcgctcgcgc gactgctcag tccagtgctc gacgtgctga tacggctctg
     6181 gcttgccgaa ggttttctcg tggccgacgt catgcagcat atgcttggcg atcaaaccac
     6241 cgagcgcgat catctcccac cttcggcgtt actgtacgcc agcctcgccg ccacgggctt
     6301 tggcattttc gtacaaacga tttgcccggt gttgctggct gccggactgt tcacgcgcct
     6361 tgccgctcta gcgctgttgt tgcaggtgct tgtcttgcaa atgcccgggc acgcgcaatt
     6421 ggcgccctac tcagtggcgc tgctcgggtg gatcgttgtg ctcgggccgg ggccgctctc
     6481 gcttgacggc atgttccgcc ctggcatcgg tgccgcggcg ctgccccttg ttggccttgt
     6541 cagccg
//
LOCUS       DATLJT010001532         1534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_373410,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001532 DATLJT010000000
VERSION     DATLJT010001532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373410"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..690)
                     /locus_tag="VM910_35080"
     CDS             complement(286..690)
                     /locus_tag="VM910_35080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67770.1"
                     /translation="MCGYSLHSVRTRPAKVGEKLVTHNFGTGTRGFAAPEDCSVAVCL
                     LPGTELAFETPVKYQTALLFRTNTVHTVAIFRQINKSVLHINHDALEFPDGKTVLLTR
                     LCEGQKATVLTLPTQSKTANKEEARRRVAHVG"
     gene            complement(838..>1534)
                     /locus_tag="VM910_35085"
     CDS             complement(838..>1534)
                     /locus_tag="VM910_35085"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HVI67771.1"
                     /translation="LYFLPTVRQSELAPDGHPKRGGFLPPVPLPRRMWAGARFEFHRP
                     LHIGEAIVRKSAIADVSTKDSRVGPLIFVLVRHEITGPAGLALVEEHHIVYREAPGSS
                     EVAPQPPAAPTGAVWRRDIHADEVMLFRYSALIFVGHRIHYDRRYATEVEGYPGLVVH
                     GPLIATLLADLLRRHSSAPPKSLHFRAISPLFDNAPFAVCGMPEPDGRASLWAQDAEG
                     ALAMKAEAVMHSA"
BASE COUNT          299 a          451 c          485 g          299 t
ORIGIN      
        1 tgggcccaag cgtgtttgcc attccgattt catcgccggg cggccatggc ggacgccgaa
       61 tgtcagtctg cgcctcggcc gccgtgatca ctgcgctcgc ggccgcgagc gcacagataa
      121 gtcgcaggat gatcattgtc gtacctcggt tgtcctgatt gcgcatcaat gtattgcgtg
      181 tccgcactag tttccggcat tcgagagtgt cccgtgctct gcgggaagga ggccgcccga
      241 gagggagcgg cctgaactct tggccgttgt acacgacaac gaatgtcagc cgacgtgggc
      301 gacgcgccgc cgggcttcct ccttgttagc cgtcttcgat tgcgtgggaa gcgtgagaac
      361 agttgccttc tgtccttcgc agagccgcgt cagcagcacg gtcttgccat cggggaactc
      421 gagcgcgtca tgattgatgt ggagcacgct cttgttgatc tggcgaaaga tcgcaacggt
      481 gtggacggta ttggtacgaa acaggagggc ggtttgatac ttgactggcg tctcgaacgc
      541 gagttcggtg ccaggaagta agcagacagc caccgaacaa tcttccggcg cggcgaatcc
      601 tcgtgtgccg gtgccgaagt tatgtgtgac gagtttctcg cccactttgg ctgggcgtgt
      661 tcgcacgctg tgcagactgt agccgcacat atcaaggaac tcctctcttg gaagcccccg
      721 atagtgcaat attatcagaa aagacgccgc gacgcatcaa ttcgcgaaca gtgccctggc
      781 cttctggccg caatcaagtg atcacgtcat cgctacgttg cgaaaagcgc gacgtcgtta
      841 tgctgagtgc atgaccgcct cggccttcat tgcaagagca ccttcagcat cttgcgccca
      901 caggcttgcc ctgccgtctg gttctggcat gccgcatacc gcaaacgggg cattgtcgaa
      961 cagcggactt atggcgcgga aatgtaaaga ttttggcggc gcgctggagt ggcggcgcag
     1021 caggtccgcg agcaacgtgg cgatcagtgg tccatgcacg acgagcccag gatagccctc
     1081 cacctcggtc gcgtagcgcc gatcataatg gatgcggtga ccgacgaaga ttagcgcgga
     1141 atagcgaaac agcatcacct catcggcatg gatatcacgc cgccagaccg cgcccgtcgg
     1201 cgctgcagga ggctgcggcg ccacttcgct ggagccgggc gcctcgcgat acacgatgtg
     1261 gtgctcctcg accaaggcga gacctgcggg ccccgtgatc tcatgcctca ccaacacgaa
     1321 gatcaatggc ccgacgcgac tatctttcgt ggaaacatcg gcgatcgcgg acttgcggac
     1381 gatagcttca ccaatgtgca acggccgatg gaattcgaac cgcgcgccgg cccacatacg
     1441 gcgtggcagc gggacgggcg gcaggaagcc gccgcgcttg gggtgtccgt cgggtgcgag
     1501 ctccgactgg cgcacggtgg gcaggaagta aagc
//
LOCUS       DATLJT010001533         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_830864,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001533 DATLJT010000000
VERSION     DATLJT010001533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830864"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1748)
                     /locus_tag="VM910_35090"
     CDS             complement(<1..1748)
                     /locus_tag="VM910_35090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HVI67772.1"
                     /translation="MIGLVRAAAICVTLALGLVQAQAADKAFKRDDLADAAIKLEAQI
                     KSEAGPVSKSAATLRTDADVAFRRNDFRSGMQILGQIVAAAPEDSGNWLRLAQSILQI
                     KPSTTSEQTFFLERASTAAFVAYLRATNPQDEADALAVLGRALSERKLWRPALDALRL
                     SLDLREVADVRGQYENLRDEHGFRLLDYTVDSDSASPRACFQFSEDLAKRTDFTPFVA
                     LAGTDKPALSSEDKQLCVEGLKHGERYNINLRAGLPSTVREGLPKSAEFNIYVRDRKP
                     FVRFTSRAYVLPHTGQRGIPVVSVNTSAVAVNVFRIGDRNLINTVIDSDFQRPISRYQ
                     LSDLGDERGVKVWTGELATATTLNQEVTTAFPVDQALGELQPGVYVMTAVPKTPGSDD
                     DYQLATQWFIVSDLGVSAFSGNDGIHVFVNSLASTDAVPHAEVRLIARNNEILATRKT
                     DDAGHVLFETGLARGEGGSSPAMLTVAGEKTDYAFLSLKSNAFDLTDRGVAGRAVPAG
                     ADAFVYAERGVYRTSETVYLTALLRDGQGNALTGSPLTLVVERPDGVEFRRIVLPDQG
                     AGGRSLSVALNPAVPSG"
     gene            2153..2229
                     /locus_tag="VM910_35095"
     tRNA            2153..2229
                     /locus_tag="VM910_35095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2187..2189,aa:Arg,seq:tct)
BASE COUNT          427 a          688 c          721 g          393 t
ORIGIN      
        1 ccgctgggaa cggcgggatt aagggcgacg gacaatgaac ggccgcccgc gccctggtcg
       61 ggcagcacga tacgccgaaa ctccacgcca tcaggacgct cgaccaccag cgtcagcggc
      121 gagccggtca gcgcgttgcc ctgcccgtcc cgcagcagcg ccgtgagata gaccgtttcg
      181 ctggtgcggt agacgccgcg ctcggcatag acgaaggcat cggcaccggc cggcaccgcg
      241 cggccggcga caccgcggtc ggtcagatcg aaggcgttcg atttgaggct caggaaggca
      301 tagtcggtct tttcaccggc aaccgtcagc atcgccggcg acgacccgcc ctcgccgcgc
      361 gccagtcccg tctcgaacag tacgtgaccg gcatcgtcgg tcttgcgggt cgccagaatc
      421 tcgttgttgc gcgcgatcag ccgcacttcc gcatgtggaa cggcgtcggt cgacgccagc
      481 gaattgacaa agacatggat gccgtcgttg ccggaaaatg cgctcacacc gagatcggag
      541 acgatgaacc attgcgtcgc gagctggtag tcgtcgtcgc tgccgggcgt cttcggcaca
      601 gccgtcatca catagacgcc gggctgcagc tcgccgagcg cctggtcgac cggaaatgca
      661 gtggtgactt cctggttcag cgtggtcgcg gtggcgagtt ccccggtcca gaccttgacg
      721 ccgcgctcat cgccgaggtc ggagagctga tagcggctga tcggccgttg gaaatctgaa
      781 tcgatcacgg tgttgatcag gttgcggtcg ccgatccgga acacgttgac ggcgaccgcc
      841 gacgtattga cgctgaccac cggaatgccg cgctggccgg tgtgcggcag cacataggcg
      901 cggctggtga agcgcacaaa tggcttgcgg tcgcgcacat agatgttgaa ctcggccgat
      961 ttcggcaggc cctccctcac cgtcgatggc agaccggcgc gcagattgat gttgtagcgt
     1021 tccccatgtt tcaacccctc cacgcaaagc tgcttgtcct ccgaggaaag cgccggcttg
     1081 tcggtgccag cgagcgcgac gaacggggtg aaatcggtgc gcttggcgag gtcttcggag
     1141 aactggaagc aggcgcgcgg tgaggccgaa tcggaatcga cggtgtaatc gagcagccgg
     1201 aagccgtgct cgtcgcgcag gttttcatac tggccgcgaa cgtctgcgac ctcgcgcaaa
     1261 tcaagcgaca agcgcagcgc atcgagcgcc ggccgccaca gcttacgctc cgacagcgcc
     1321 cggccgagca ccgcaagcgc gtccgcctcg tcctggggat tggtggcacg cagataggcg
     1381 acgaacgccg cggtcgatgc gcgctccagg aagaaggtct gctcgctcgt ggtcgagggc
     1441 ttgatctgca aaatgctctg cgccaatcgc agccagttgc cgctgtcttc cggcgctgcg
     1501 gccacgatct ggccgaggat ctgcatgccg ctgcggaaat cgttgcgcct gaaggcgacg
     1561 tccgcatcgg tgcgcagcgt tgcagcagac ttcgacacgg gccccgcctc gctcttgatc
     1621 tgggcctcca gcttgatcgc ggcgtcggcg agatcgtcgc gcttgaacgc tttatccgcc
     1681 gcctgcgcct gaaccagacc aagcgccagc gtgacgcaaa tggctgcggc gcgaaccaaa
     1741 ccgatcatgg ggagctcctt acgcgcaacc gacgtcgcgt ggtcgataaa ggtgcgcgaa
     1801 atggtgacgg gctgatggcg gcatcaagaa ggtagcagaa agtcgcatgc gacatggcgc
     1861 agccaaaagc ttaaccgcgc cggcagtcgg ccacgcggca atgattgacc ggcggaccga
     1921 aaatgaacgg gtcgccgtcc taactttgtc gtttcggcgc aggaaacggt tcgccgcgaa
     1981 tgcccgattt taatgttcac aagcgatctc cggaggggct cctgcaaatc ttaaaatcaa
     2041 ggggcactcg caaatatcca actccaatgc ggttttggat ttgacattcg tccgacttgg
     2101 cgaaaaagca gatttttcat atctgcagtg ctatgaaggc ggccgccgag acggtcccgt
     2161 agctcaactg gatagagtag cggatttcta ctccgcgggt tgcaggttcg agtcctgccg
     2221 ggatcgcca
//
LOCUS       DATLJT010001534         3636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2212541,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001534 DATLJT010000000
VERSION     DATLJT010001534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212541"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VM910_35100"
     CDS             <1..607
                     /locus_tag="VM910_35100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI67773.1"
                     /translation="LRKVSKFNDAVQAWMFNPDALAPTFPESAITKPFPFNGYYDVED
                     APEVDGKTWKLEVRGLVDNKKSWTLDELYQLPQVKQVTRHICVEGWSAIGSWTGTPLR
                     DFLKLIGADMRAQYVWFQCADSEGYNSPLDMRTALHPQTQMTFKFADEILPRAYGYPM
                     KIRVPTKLGFKNPKYVVSMEVTNDYKGGYWEDQGYNSFSGN"
     gene            complement(666..1853)
                     /locus_tag="VM910_35105"
     CDS             complement(666..1853)
                     /locus_tag="VM910_35105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HVI67774.1"
                     /translation="MHAPQASSSATADPARLILTLSVGSIVGIGICRFAYALVLPDMR
                     DSLHWSYSAAGFMNTVNAAGYLIGALLSSRLIRRFGWSASIRGGTLACLASLAACATS
                     DNFIILSLARLVAGLGAAAAFIGGGALAAHLAQAHPERANFLLSLFYAGPGLGIVASG
                     LIAPSVLQAFGPGSWWIVWWALTALAVVLTVPLLLARIESKTGLTGTGHASFAIAPIL
                     IYLGGYFLFGAGYIAYMTFMIAYVRDAGGGPAAQAGFWSLIGLGAFVTPWVWRGVLGL
                     DRGGLATAIILSVNTIAVALPMFGHSALLLAISALVFGVAFFAVVSSTTAFVRYNYPP
                     AVWPTAIAAMTIAFGIGQTLGPIVVGAVSDALGSVAYALNVSAALLGLGAIASACQRK
                     LSK"
     gene            1982..3094
                     /gene="leuB"
                     /locus_tag="VM910_35110"
     CDS             1982..3094
                     /gene="leuB"
                     /locus_tag="VM910_35110"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HVI67775.1"
                     /translation="MATHKLLLLPGDGIGPEVMAEVKRLLEWLNESGIARFETELGLV
                     GGSAYDAHKVSITDGTMGRAKAADAVIFGAVGGPKWDGVPYEARPEAGLLRLRKELGL
                     FGNLRPAVCYPALADASSLKREAVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAV
                     DTQVYDTYEIERIGRVAFDLARKRRNKVTSMEKRNVMKTGVLWHEVMTQLHAREYRDV
                     VLEHQLADSGGMNLVKSPKQFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDA
                     KSKRRKALYEPVHGSAPDIAGKGLANPIAMIASFGMALRYSFDMGALADKLDAAIAAV
                     LAKGLRTADIRSEGTTPVSTAQMGEAILGELKKLHA"
     gene            complement(3210..3593)
                     /locus_tag="VM910_35115"
     CDS             complement(3210..3593)
                     /locus_tag="VM910_35115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67776.1"
                     /translation="MTAKFLAVRHFSLLLAAGAMMAASLATEAGAQSAPQYDSRGRPI
                     YGSGGPNYSYQQGPHTRIYVTKRSWLDGGTEVLPGDRKFMDYAFPSEYGLPDFARENN
                     NRPIDRQPTNPPSDMGGFPTRFPLY"
BASE COUNT          756 a         1121 c         1178 g          581 t
ORIGIN      
        1 gctgaggaag gtttcgaaat tcaacgacgc cgtgcaggcc tggatgttca atccggacgc
       61 gctggcgccg acctttccgg agagcgccat cacaaagccg ttcccgttca acggctatta
      121 cgatgtcgaa gacgcgccgg aagtcgacgg caagacctgg aagctcgaag ttcgcggcct
      181 cgtcgataac aaaaagtcct ggacgttgga cgagctctac caactcccgc aggtcaagca
      241 ggtcacacgc catatctgcg tcgagggctg gagcgcgatc ggtagctgga ccgggacgcc
      301 gctccgcgat ttcctgaaac tgatcggcgc cgatatgcgt gcccagtatg tctggttcca
      361 gtgtgccgac agcgaagggt acaattctcc gctcgacatg cgcacggcgc tgcatccaca
      421 gactcagatg acgttcaagt tcgccgacga gattctgccg cgggcctatg gctatccgat
      481 gaagatccgg gtgccgacaa aactcggatt caagaatccg aaatatgtgg tctcgatgga
      541 agtcaccaac gactacaagg gtggctactg ggaggaccag ggttacaatt cgttcagcgg
      601 gaattgacca tactacgcag tcacgtcagg cgatatatcg catcgcgccc cgggaacgat
      661 gttggctact tgctcagctt tctctgacac gccgatgcaa tcgcgccgag ccccagcagg
      721 gccgcggaaa cgttaagcgc ataagcgacg ctgccgagcg catcgctgac agcgcccacc
      781 acgatcgggc cgagcgtctg accgatcccg aaggcgatcg tcatcgccgc gatcgcggta
      841 ggccacaccg cgggcggata gttgtaccgc acgaaggcgg tggtcgagct cactaccgcg
      901 aagaaggcga cgccgaacac cagtgcggag atcgcaagca agagagcgga gtgaccgaac
      961 atgggcagag cgacagcgat cgtgttgacc gaaagaatga ttgcggtcgc aagtccgccg
     1021 cggtcgagcc cgagcacgcc tcgccacacc caaggcgtga cgaatgcacc caagccgatc
     1081 aagctccaga aaccggcctg cgccgccggg ccgccgcccg catcgcgcac atacgcgatc
     1141 atgaaggtca tgtaggcgat gtagcccgcg ccgaacagga aatagccgcc gagatagatc
     1201 aggatcggcg caatcgcaaa gctcgcgtga cctgtcccgg tcagacctgt cttgctctcg
     1261 atccgcgcca ggagaagcgg gacagtcagg acgacggcaa gcgccgtcag cgcccaccat
     1321 acgatccacc aggagcccgg tccgaatgcc tgcagcacgg agggcgcgat caggccggat
     1381 gcaacgatgc cgagcccggg accggcgtag aacaggctca acaggaagtt tgcccgctcg
     1441 gggtgcgctt gcgcgaggtg tgcggcgagc gcgccgcccc cgatgaacgc cgccgctgct
     1501 ccgagccccg cgaccagccg ggccagactc aggatgatga aattgtccga ggtcgcgcag
     1561 gctgccagcg aggcgaggca ggccagcgtg ccgccacgaa tcgaggccga ccagccgaag
     1621 cgccggatca ggcgcgagga cagcagtgcg ccgatgagat aaccggcagc gttgacggta
     1681 ttcatgaaac ccgccgccga gtaggaccaa tgcagcgaat cccgcatgtc cggcagcacc
     1741 agcgcgtaag cgaaacggca gatcccgatg ccgacgatcg agccgacgga cagcgtgagg
     1801 atcagccgcg caggatcggc tgtcgcggaa gaggaagctt gaggggcgtg caagggatgc
     1861 tcccgcggca aatattctga caggctcctg cgtccttgca caggcgcgtc atcggcaatg
     1921 gtgtcttgcc aatcgcgcaa cgcggcatta gcactccgcg cgaccagaga ggaaacctgc
     1981 catggcgacc cacaaactgc tgcttcttcc cggcgacggc atcggccccg aagtcatggc
     2041 ggaggtgaag cgcctgctcg agtggctgaa tgaaagtggc atcgcgcggt ttgagaccga
     2101 actggggctg gtcggcggct ccgcctatga cgcgcacaag gtatccatca ccgacgggac
     2161 gatgggccgc gcgaaggcgg ccgacgccgt catctttggc gcggtcggtg gaccgaaatg
     2221 ggacggggtg ccctatgagg cgcggcccga ggccggcctg ttgcggctgc gcaaggaact
     2281 tggcctgttc ggcaatctgc gccccgcggt gtgctatccg gcactggcgg acgcctccag
     2341 cctgaagcgc gaggcggtcg aggggctcga catcatgatc gtgcgcgagc tcaccggcgg
     2401 cgtctatttc ggcgagccga agaccatcac cgatctcggc aacggccaga agcgcgccgt
     2461 cgatacgcag gtctacgata cctacgagat cgagcgaatc gggcgcgtcg ccttcgatct
     2521 cgcgcgcaag cgccgcaaca aggtgacctc gatggagaag cgcaacgtca tgaagacggg
     2581 cgtgctctgg cacgaggtga tgacgcagct ccatgcgcgc gaatacagag atgtcgtgct
     2641 ggagcatcag ctcgccgatt ccggcggcat gaacctcgtc aaatctccga aacagttcga
     2701 cgtgatcgtc accgacaatc tgttcggtga catgctgtcc gacatcgccg cgatgctcac
     2761 cggttcgctc ggcatgctgc cgtccgcctc cctcggcgag gtcgacgcga agagcaagag
     2821 acgcaaggcg ctctatgagc cggtccatgg ctccgcgccc gacatcgcgg gcaaaggcct
     2881 cgccaatccg attgcgatga tcgcgtcgtt cggcatggcg ctgcgctatt ccttcgacat
     2941 gggggcgctc gccgacaagc tcgatgccgc gatcgcggcc gtgctcgcga aaggtctgcg
     3001 cacggcagat atcaggtccg agggcactac gccggtctcg actgcgcaga tgggcgaggc
     3061 gatcctgggc gagctaaaga agctgcacgc atagcaacag ccgtcatgtc ccggctggca
     3121 ggatcaacct gactttcatg cggagcaggc caatggaccc ctgagacagg ctcacgtcca
     3181 cgctgacgtc ggcattgtgt tgtgatcgct caatacagcg ggaatcttgt cggaaagccg
     3241 cccatatccg atggcggatt ggtgggctgg cgatcgatcg gacggttgtt gttctcgcgt
     3301 gcgaaatccg gcaacccgta ctcggacggg aaggcgtagt ccatgaattt gcggtcgccc
     3361 ggcaacacct cggtgccgcc atccagccag gagcgcttgg tcacgtagat gcgggtgtga
     3421 ggcccctgct gataggagta attgggtcct cccgagccgt aaatgggacg gccacggctg
     3481 tcatattgcg gggcagattg cgcgccggcc tcggtggcca ggcttgcggc catcatcgcg
     3541 ccggccgcca acaacagcga gaagtgcctc acggcgagga attttgcggt cattagatcc
     3601 tcatttgggc tggcaagaag gccaggcgcg atctcg
//
LOCUS       DATLJT010001535         3416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1754584,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001535 DATLJT010000000
VERSION     DATLJT010001535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754584"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VM910_35120"
     CDS             complement(<1..471)
                     /locus_tag="VM910_35120"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67777.1"
                     /translation="MSSQAERRQLTVMFCDLVGSTELSARLDPEDLREVIGAYHRTVA
                     AAVTRFDGFVAKYMGDGVLAYFGYPQAHEDDAERAVRAGLGLIDAVARLDIRSVDLQA
                     RVGISTGLVVVGDLIGEGSAQEQSVVGETPNLAARLQALAAPDQIVIAENTWRQV"
     gene            959..1387
                     /locus_tag="VM910_35125"
     CDS             959..1387
                     /locus_tag="VM910_35125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67778.1"
                     /translation="MLDETNWTIDLALAGNWPPVTDAEIEAVAWGWWSNFFTTRLREL
                     RRTGRACDDRSEWALANEAELRRSVEAFIAGPHPLDTFGQPKGDLAKVLDDPRRAVSL
                     LSNKDAIARLLHECRENHQEHAGNFADRAARAQLRNGARS"
     gene            1605..2345
                     /locus_tag="VM910_35130"
     CDS             1605..2345
                     /locus_tag="VM910_35130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67779.1"
                     /translation="MIRKPSGFVGFDDLARLTRQNVEDWIELLMETEVAPGEKMAPKT
                     VEQHLIQLKALANFAVDKAIISVNPAGKVRFSARVRTKIRAFTNAEARVVLEAARHET
                     TDYKRWLPWICCFTGCRLDEPAGAAVPDIERVGSYWVLNVRLDHRHEGASIKNDSSVR
                     RVPLHPKLWGRARLCPKPVRERAVVPVATARQVRLEGWQRYETDRPDRARAGRCHAIA
                     RRQTLIAEPLMATPVDRGMPSDSYPPGH"
     gene            2302..2481
                     /locus_tag="VM910_35135"
     CDS             2302..2481
                     /locus_tag="VM910_35135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67780.1"
                     /translation="MIEECRRIPIRQDIEDALTGHSQEGSGPGYGEFAINDMLGPAIY
                     QTRSPFDVELSAAAK"
     gene            2729..3097
                     /locus_tag="VM910_35140"
                     /pseudo
     CDS             2729..3097
                     /locus_tag="VM910_35140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          680 a          995 c         1101 g          640 t
ORIGIN      
        1 gacttgtcgc caagtgttct ccgcgatcac gatttgatca ggagcagcga gcgcctgcag
       61 ccgggcggcg aggttggggg tctcgccgac gaccgattgc tcctgcgccg agccctcgcc
      121 aatcagatcg cctaccacca ctagcccggt ggagatcccg acgcgcgctt gaaggtcgac
      181 ggatctgata tcgaggcgag cgacggcgtc gatcagcccc agccccgctc ggaccgcgcg
      241 ctcggcatcg tcctcatgcg cttggggata cccaaaatag gccagaacgc cgtcgcccat
      301 atatttcgcg acgaagccgt cgaacctggt cacagccgcc gcgacggtac gatgataggc
      361 gccgattacc tcacgcagat cctcaggatc gagccgggca gagagctcgg ttgagccaac
      421 aagatcgcag aacatcacgg tcagctggcg gcgctccgcc tggctggaca cggcgtcggc
      481 cggaactggt cccgccgcca ttagtgggtt agggccatac ctactccgat atggagcgac
      541 ggcgggaact ctcgtcccac aaattagccc aataaatctt ggtggtacga taggcaaatt
      601 gcttgggcaa atgactcccg atatctcaag taacctattg atttgtatga gatgatgatt
      661 gcgtagtgcc agtccgcccc tgggcaccac gcttaacgaa accctttaga ttccctttag
      721 atattccgga tcaatcattt gggagtcacg accggagtca caaagggagt cacaaatggc
      781 cggaaggacg gctcgctcgt gtggaataga gcggtatctg cattggcgtc ctgaaaaggg
      841 acgatggtgg ttccgccgtc gcgtgtccgg cgatctcgtc gcaatcatcg gaaaggccga
      901 gtggcgtcag acgcccgcag cgcgaagccg gcccgaggcc gagcgtgagg ctatgcggtt
      961 gctcgacgag accaactgga ccatcgatct cgctttggcc ggcaactggc ctcctgtgac
     1021 cgatgcagag atcgaggcgg tagcttgggg ttggtggtca aatttcttca ccacccggct
     1081 gcgcgaactc cgtcgaactg gccgggcctg tgatgatcgt tcagaatggg cgctcgccaa
     1141 cgaggctgaa ctgcggcgat cggttgaagc gttcatcgct ggcccacatc cgctcgatac
     1201 cttcggtcag cccaaaggcg atctcgctaa ggtgctcgat gatccgagac gtgcagtgag
     1261 tttgttgagc aacaaggatg cgatcgcacg gctgctacac gaatgtcgtg agaaccatca
     1321 ggaacacgct ggcaacttcg ccgaccgtgc ggcgagagcg caacttagaa acggagcgcg
     1381 ttcttgaggc gatccgcgta ggcgcgatcg aaccgcgcac gctggtcaac ataatcgatc
     1441 gtcccgtgtc atcggtgttc caagcatccg cgcccgtcat cggtgtggct cccataccgt
     1501 ttaacgaccc ggatggtggc gacgacctga tagcgaagtg ggcgaagtgg ccggggaaaa
     1561 ggggcaaacc gccgggagcg aagaccgtct acgaggcccg gcggatgatc cgcaaacctt
     1621 cggggttcgt cggttttgac gatctcgcac gtctgacacg gcagaatgtc gaagactgga
     1681 tcgaattgct catggagacc gaggtcgctc ccggcgagaa gatggctccg aagactgtcg
     1741 agcagcattt gatccaactc aaggcgctcg ccaacttcgc cgtcgacaag gcgatcatct
     1801 cggtcaatcc agccgggaag gtcagattct cggcacgggt taggacgaaa atccgcgcct
     1861 tcacgaacgc tgaagccaga gtcgtgctgg aagccgcgcg gcacgaaacg accgactaca
     1921 aacggtggct gccatggatc tgctgcttca ccggctgccg gctcgacgaa cccgccgggg
     1981 cagctgtccc tgacatcgag cgggttggct catattgggt gttgaacgtc cggctcgatc
     2041 accggcacga aggcgcatca atcaagaacg actcgtcggt gcgccgcgtg ccgttacatc
     2101 cgaagctgtg ggggagggct cgattatgtc cgaagcctgt ccgagaacgg gccgttgttc
     2161 ccgtcgctac ggcccgacaa gttcggctcg aagggtggca acgctacgaa acggatcggc
     2221 ccgatcgtgc gcgggctggc cggtgtcatg ccatcgcccg cagacaaaca cttatcgccg
     2281 aaccactcat ggcgacaccg gttgatcgag gaatgccgtc ggattcctat ccgccaggac
     2341 attgaggacg cgctgaccgg ccattctcaa gaaggcagcg ggccgggcta tggcgaattc
     2401 gccatcaacg atatgctcgg cccggcgatt tatcagacac gctctccctt cgatgtggag
     2461 ctttcagcag ccgccaagtg aaggctctgc gagcgccgaa gcgacggcat cacgctttac
     2521 cggcgggatt tgcacccatg tcgggccgcg acggcgatct cgtctagcag tcccgcatcg
     2581 ttgttcctga aattgccgac ccgcctatcg accagatggg cgtctatcaa ctcgttcgga
     2641 aacggtagca ggatggcgag caactgatcg gcgggccttg tcaggataat cgcatggcag
     2701 gggagagagg tgcgagtagg ctgaccggat gatggagctc tcctaccgtc gtcaccgctt
     2761 tccgcccgtt gtcatccagc atgccgtgtg ggtctacctg cgtttcacgc gcagctaccg
     2821 agacgtcgag gatctccccg ccgagcgcgg cctcgacatc tcctacgaga ccgtccggag
     2881 ctgggtgttg aagttcggac cagtgatcgc gcggcggtta acgcggcgcc gacctcgccc
     2941 gagcgatcga tggcatctgg acgagatggt cgttcggatc cccggcaagc ggatgtacct
     3001 gtggcgagcc gtcgaccacg agggcgaggt cctcgacatg ctggtccaga gtcgccgcga
     3061 cagccgggcg gcgttgcgcc tgatgcgcaa gcttcttagt ggcttacggt cgaagtgttt
     3121 gcatcttgtg agcagattgg tcagacgagg gccggaataa tggtgtgttc ggtgaggcgc
     3181 cgaccggcag cgatggcggc gcggccgggg cgagtgagat agtaacgatg gtgccggtga
     3241 cgcgtttaat gatgccgagc tggcgcaggc gtgcgagttg tcgggtcagg gtagcgggag
     3301 agcgcccggc aagcattggc aggaggttgg cgcggcggag tccggcgatt cgtgggccct
     3361 catggaaccg gtgttccgtc tcaacgggcg tggcgcggtc gttcgatgat tgatag
//
LOCUS       DATLJT010001536         4422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1523163,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001536 DATLJT010000000
VERSION     DATLJT010001536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523163"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VM910_35145"
     CDS             <1..107
                     /locus_tag="VM910_35145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HVI67781.1"
                     /translation="LDVGEIVVADAGSSLHDGDQVKTMFADELDRQVR"
     gene            107..3271
                     /locus_tag="VM910_35150"
     CDS             107..3271
                     /locus_tag="VM910_35150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI67782.1"
                     /translation="MAINISAWSIRNPLPSVVFSIILLVLGWVSFTKLAVTRLPSADI
                     PVISVAVSQFGAAPAELESQVTKTIEDGVSGVEGVRHISSSITDGLSLTTIQFALETN
                     TDRALNDVKDAVTRVRANLPQNVNEPLIQRVDVIGLPIVTYAAISPGKTPEQLSYFVD
                     DVVKRALQGVRGVAQVERIGGVEREILVSLDPDRLQAAGLTAVNVSQHLRGTNEDLAG
                     GRAEIGKNDQAIRTLAGAKTLNDLAGTMIPLFGGGEVRLDDLGTVTDTIADRRTFARF
                     NGEPVVALGIKRSKGASDVVVAGLVQKRIDALKAQYPDVDLKMIDTSVKFTKGNYEAA
                     ISTLFEGAILAVIIVLLFLRDIRATIIAAISLPLSIFPAFWAMDILGFSLNLVSFLAI
                     TLSTGILVDDAIVEIENIVRHMRMGKSPYRAALEAADEIGLAVIAISLTIIAIFAPAS
                     FMSGIAGQFFKQFGITVSVQVFFSLLAARFVTPVLAAYFLKPHTHDEPPPGRILMSYH
                     RLVSWSVRHHFITVLIGIAIFAASIWSIKLLPQGFLPAQDTARSLLAMELPPGSQLSY
                     TEQVTEDIVARLRKRPEVKSVFVDGGRVPPGTLEVRRAALIINYTPKNTRSVTQRELE
                     LSISNELENVPDIRFWFLDENGLRAISLVVTGTDSNIVNNVANELATQMKRIPIISNV
                     TPETSLDRPELRIRPRADLAARLGVSTEILSQTIRVATIGDVGPALAKFDAGDRLVPI
                     RVQLEDSARSNLRTLQQLRVPLGEHGEKGGVPLEVVAEIKLDQGPTSINRYDRERQAT
                     VAADLVGSAALGDATKKIYDLPVMKSLPKGVKVSPSGDAESLNELSQGFATAITAGLM
                     MVYAVLVLLFGTFLQPITILFSLPLSIGGAIAALLLTGKQLTTPVWIGILMLMGIVTK
                     NAIMLVEFAIESIHSGQTREDAMIDAGMKRARPIVMTTIAMIAGMTPSALAVGAGGEF
                     RSPMALAVIGGLLFSTVLSLVFVPAMFMVMDDIGSLIWRFGKKLIVSSADAEHGGDHH
                     AAPPGGQTQTPPAPPPKIVSPAAE"
     gene            complement(3286..4221)
                     /locus_tag="VM910_35155"
     CDS             complement(3286..4221)
                     /locus_tag="VM910_35155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HVI67783.1"
                     /translation="MNRLRRSGRAHSSARAASAPGSGPTSHIVTVGDCLGVLAKIPSA
                     SIQLVICDPPYNINVASWDDHAHYVDWAGKWLGEVERVLKPSGNFVLFGGLQYQGEAG
                     SGDLLGLMSWMRRHSRMGLANLIIWNYPNGMSAQRFFANRHEEIAWFARTPKYYFDLD
                     AVREPLDAKRLKTYKRDKRLNHDSLDKGINPTNVWKVPRLNGNSKERVGHPTQKPREL
                     IARLVRALSYPGSTVLDFFAGSCVTARVAIETRRHSISSDVDKTLRTYLARQLKQVGN
                     DCARPADYTLIEGQDWSGHPVFQPARNAKTEPVSM"
     gene            complement(4294..>4422)
                     /locus_tag="VM910_35160"
     CDS             complement(4294..>4422)
                     /locus_tag="VM910_35160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI67784.1"
                     /translation="GAARESVNRQLQAWRKDGILDLQRGRILLQNLTRLTAIARNE"
BASE COUNT          774 a         1469 c         1340 g          839 t
ORIGIN      
        1 gcctcgacgt cggcgaaatc gtcgtggccg atgccggctc ttcgctgcat gacggcgacc
       61 aggtcaagac catgttcgcc gacgaactcg accgacaggt acgctgatgg caatcaatat
      121 ctcggcttgg tcgatccgca acccgcttcc ctcggtggtc ttctcgatca tcctcctggt
      181 gctcggttgg gtcagcttca ccaagctcgc ggtgacccgc ctgccctccg ccgacattcc
      241 ggtcatctcg gttgcggttt cgcaattcgg cgcggcgccc gcggaactgg aatcgcaggt
      301 cacaaagacg atcgaggacg gcgtttcggg cgtcgaaggt gttcgccata tttcctcctc
      361 gatcaccgac ggactgtcgc tgacgacgat ccagttcgcg ctggaaacca ataccgaccg
      421 cgcgctgaac gacgtcaagg acgcggtcac gcgggtgcgc gcaaacctgc cgcagaacgt
      481 caacgagccg ctgatccagc gcgtcgacgt gatcggcttg ccgatcgtca cctatgctgc
      541 gatctcgccc ggcaagacgc cggagcagtt gtcctatttc gtcgatgacg tggtcaaacg
      601 cgccctccag ggcgttcgcg gcgtcgctca ggtcgagcgc atcggcggtg tcgagcgcga
      661 aatcctggtg tcgctcgatc cggaccggtt gcaggccgcg gggctgacgg ccgtcaatgt
      721 cagccagcac ctgcgcggca caaacgagga cctggccggc ggccgggccg aaatcggcaa
      781 gaacgatcag gcgattcgca cgctcgccgg cgccaagacg ctgaacgatc tcgccggcac
      841 catgatcccg ctgttcggtg gcggcgaagt ccggctcgac gatctcggca ccgtcaccga
      901 caccatcgcc gaccggcgca ctttcgcccg cttcaacggc gagccggtgg tggcgctcgg
      961 catcaagcgc tcgaagggcg cttccgacgt cgtggttgcg ggcctcgtgc agaagcgcat
     1021 cgatgcgctg aaggcgcaat atcccgacgt cgacctgaag atgatcgaca cttcggtgaa
     1081 attcaccaaa ggcaattacg aggccgcgat ctcgactttg ttcgaaggcg ccattctcgc
     1141 cgtcatcatc gtgcttttgt tcctgcgcga catccgcgcc accatcatcg ccgcaatctc
     1201 gctgccgctg tcgatcttcc ccgccttctg ggcgatggac atcctcggct tctcgctgaa
     1261 cctcgtctcc ttcctcgcca tcacgctgtc gacgggtatc ctcgtcgacg acgccattgt
     1321 cgagatcgag aacatcgtcc gtcatatgcg gatgggcaag tcgccttacc gcgcggcgct
     1381 cgaagcggcc gacgagatcg ggctggccgt catcgcgatc agtctcacca tcatcgcgat
     1441 cttcgcaccg gcaagcttca tgtccggcat cgccggccag ttcttcaagc agttcggcat
     1501 cacggtgtcg gtgcaggtgt tcttctcgct gctcgccgcg cgcttcgtca ccccggtgct
     1561 cgccgcatac ttcctcaagc ctcataccca cgacgaaccg ccacccggcc gcattttgat
     1621 gagctaccac aggctcgtga gctggtcggt gcggcaccac ttcatcaccg tcctgatcgg
     1681 catcgccatc ttcgcggcgt cgatctggag catcaagctt ttgccccagg gcttcctgcc
     1741 ggcgcaggac accgcgcgct cgctccttgc gatggagctg ccgcccgggt cccagctttc
     1801 ctacaccgaa caggtcaccg aagacatcgt ggcgcgcctg cgcaaacggc cggaggtgaa
     1861 gagcgtgttc gtggacggcg gtcgcgtccc gccaggcacg ctcgaggtcc ggcgcgccgc
     1921 gctgatcatc aattacacgc cgaaaaacac ccgatccgtc acccagcgcg agctcgagct
     1981 gtcgatcagc aacgagctcg aaaatgttcc ggacatccgc ttctggttcc tcgacgaaaa
     2041 cggtctgcgt gcgatttcgc tggtcgtcac cggcaccgac agcaacatcg tgaacaacgt
     2101 cgccaacgag ctggcgacgc agatgaagcg aattccgatc atctccaacg taacgcccga
     2161 gacttcgctc gaccgccccg agcttcgcat ccggccgcgg gccgaccttg cggcccggct
     2221 cggcgtctcc accgagatcc tgtcgcaaac catccgcgtc gcgacgatcg gcgacgtcgg
     2281 accggcactt gccaagttcg atgccggcga tcgtctggtg ccgatccgcg tccagctcga
     2341 agattcggca cgcagcaatc tgaggacgct gcagcaattg cgcgtgccgc tgggcgagca
     2401 cggcgaaaag ggcggcgtgc cgctcgaggt ggtcgccgaa atcaagctcg atcagggccc
     2461 gaccagcatc aaccgctacg accgcgagcg gcaggcgacg gtcgcggccg acctggtcgg
     2521 ctccgccgcg ctcggcgacg ccaccaagaa gatctacgat ttgccggtca tgaagagcct
     2581 gcccaagggc gtcaaggtga gcccgtcggg cgacgccgaa agcctcaacg aattgtcgca
     2641 aggcttcgcc accgcgatca cagccggcct gatgatggtc tacgcggtgc tggtgctgct
     2701 gttcggcact ttcctgcagc cgatcaccat cctgttttcg ttgccgctct cgatcggcgg
     2761 cgcaatcgcc gcactgctat tgaccggcaa gcaactcact acgccggtct ggatcggcat
     2821 cctgatgctg atgggcatcg tcacgaagaa cgcgatcatg ctggtcgagt tcgcgatcga
     2881 atcgatccac tctggccaga cgcgggaaga tgcgatgatc gatgccggca tgaagcgcgc
     2941 tcgcccgatc gtgatgacca cgatcgcgat gattgccggt atgacgccga gcgcgctcgc
     3001 ggtcggcgcc ggcggcgaat tccgctcccc gatggcgctc gccgtgatcg gcggcctgtt
     3061 gttctcgacg gtcctgtcgc tggtgttcgt gcctgcgatg ttcatggtga tggacgacat
     3121 cggctccctg atctggcgtt tcggcaagaa actcatcgtc tcctcggccg atgccgagca
     3181 cggaggcgac catcatgcgg caccgccagg aggtcagacg cagactccac ccgcgccgcc
     3241 gccgaagatc gtctcgcctg ccgcggagta ggcagcacgc gtcattcaca tcgagacagg
     3301 ctccgtcttc gcgttcctgg ccggctggaa cacgggatgg cctgaccagt cctgaccttc
     3361 gatcaaggtg tagtcggcag gcctcgcgca atcgttgccc acctgcttaa gctgccgcgc
     3421 gagataggtc cggagcgtct tgtccacgtc ggaggagatc gaatgccgcc ttgtttcgat
     3481 cgcgacgcga gcggtcacgc agctgccggc gaaaaagtcc agcaccgtcg atcccggata
     3541 cgacaacgcg cggacgaggc gcgcgatcag ctccctcggc ttctgcgtcg gatgaccaac
     3601 gcgctccttt gaattgccgt tcagccgcgg cactttccag acattggtcg ggttgatccc
     3661 cttgtcgaga ctatcgtgat tgaggcgctt gtcgcgctta taggttttaa gccttttggc
     3721 atcgagcggc tcgcgcaccg catcaagatc gaaatagtat tttggtgtcc tcgcaaacca
     3781 cgcaatctct tcatgccggt tggcgaagaa gcgctgcgcg ctcatgccgt tcggataatt
     3841 ccagatgatg agattggcga gacccatcct gctgtggcgg cgcatccagc tcatcagacc
     3901 gagcagatcg ccgctgccgg cttcgccctg atactgcagg ccgccgaaca gcacgaaatt
     3961 gcctgacggt ttcaggacgc gctcgacctc gccaagccat ttcccggccc aatcgacata
     4021 gtgcgcgtgg tcgtcccagc ttgcgacatt gatgttgtag ggcgggtcgc agatcacgag
     4081 ctggatcgat gccgatggga tcttcgccag cacgcccagg caatcgccga ccgtgacgat
     4141 gtgcgacgtg ggaccactgc ccggtgccga cgctgcgcgc gcggaggaat gcgcgcgccc
     4201 gctcctgcgc aggcgattca tgccgatgtg cccggtgttg cgatgggatc ggttcggcat
     4261 cttggaacag gtgataaggc gctcgcgccg atgctattcg ttgcgcgcga tcgcggtgag
     4321 ccgggtcagg ttctgcagga ggatgcggcc gcgttgcaag tccaaaatgc cgtccttgcg
     4381 ccacgcctgc agttggcggt tgacgctttc gcgcgccgcg cc
//
LOCUS       DATLJT010001537         3879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_831228,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001537 DATLJT010000000
VERSION     DATLJT010001537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831228"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VM910_35165"
     CDS             <1..833
                     /locus_tag="VM910_35165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67785.1"
                     /translation="TKTPMVVMAAATAIVTERSPYIVRSSFPQAAPVVIMADWAAKHG
                     LKKVATVVSDFAPGHDSETYFKQTFTRAGGEVPLALRVPLLNPDFAPFLQRVRDAAPD
                     GVYVFIPAGQAATMMRQFVERGLDKSGIRLFGAGDITDDDLLNNMGDVALGIETAYFY
                     SAAHPSEKNKAFVEGVKRANNGMRANFFGVSGYDGMHLICEAIRKTGGKTDGDSFIGA
                     VKGMSWESPRGPMTIDPETRDVIHNVYMRRVEKVNGELWNIEFATVENVKDPVKAARK
                     "
     gene            957..1493
                     /locus_tag="VM910_35170"
     CDS             957..1493
                     /locus_tag="VM910_35170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67786.1"
                     /translation="MQSKDEEVTPRYFNPTWAPTLSDEARKAVNAAFDAMSAWRIETA
                     SNNEKNSEQVIEKLAAAARALGWPEQIVDATRAQMQGITKMQIQTMDQMMDAWEEQIR
                     SPNAMTGSPSAMLSKLKTLPGFSPAGSWPSADALAATNPMEFWMQWAEQWQKACTDAM
                     ALWDKAGQPGYGVGPRRR"
     gene            1525..1980
                     /locus_tag="VM910_35175"
     CDS             1525..1980
                     /locus_tag="VM910_35175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67787.1"
                     /translation="MRLFAVALTLSLAAATAASAQTATPDSENGRYSFNPVADGVLRL
                     DTRTGQVSQCSRSDAGWACKVVPDERSALETEIVRLQGENATMKKELLSRGLPVPGVS
                     APSGAKPGEGELKLPSDAEIDKVVAFLEKVWRRLIEMGKNVQKDVEKKN"
     gene            2429..3064
                     /locus_tag="VM910_35180"
     CDS             2429..3064
                     /locus_tag="VM910_35180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVI67788.1"
                     /translation="MFVSGKYVNTLEPDDLKKRNRAWAARMTSEDPDFFRRLERQQAP
                     HYLWIGCSDSRVPANTIVGLDPGELFVHRNVANLASPQDANYLSVLQFAVDVLRVQHI
                     MVVGHYGCGGVSAAVDGRRRGLVDHWLHPIRELSRRHSEELEALRDEHSRLDRLCELN
                     VVQQVKNVASDVFVLDAWARGQNLSVHGWVYSIGNGLIKDLNVTVSAPGQC"
     gene            3064..3225
                     /locus_tag="VM910_35185"
     CDS             3064..3225
                     /locus_tag="VM910_35185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67789.1"
                     /translation="MAAAMGASPIACSKVVRKIGLSWMRSLWTAPAKTGVNALNDALW
                     RNPGYHATR"
     gene            3353..>3879
                     /locus_tag="VM910_35190"
     CDS             3353..>3879
                     /locus_tag="VM910_35190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67790.1"
                     /translation="MLTLRTLAIVIAGVGVFAGLPLTPAQACDDDRYPCPIRVPQEVD
                     APAQATPSVQPQKKASHAARPSEKAQAKREAPPAAARAKPSKPAAPEQAADPIAQKAA
                     EAPPALVPSPPADQPLNAASRGESPVAAAGTAWPVLPTTEDAGASAPGAAPAATSADA
                     TEAANTTAVTTAVTNA"
BASE COUNT          769 a         1170 c         1279 g          661 t
ORIGIN      
        1 agaccaagac gcccatggtg gtcatggcgg cggccaccgc gatcgtgacc gagcgctcgc
       61 cctatatcgt gcgctcgagc tttccccagg ctgcgccggt cgtgatcatg gccgattggg
      121 cggccaaaca tggcctcaag aaagtggcta cggtcgtttc cgacttcgca cccggccacg
      181 attccgaaac gtatttcaaa caaacgttta cccgcgccgg tggtgaagtc cccctggcgt
      241 tgcgggtgcc tctgctcaat ccggatttcg cgccgttcct gcagcgcgtg cgcgatgccg
      301 ctccggacgg ggtttacgtc ttcattccgg ccggccaggc cgcgaccatg atgcggcaat
      361 tcgtggagcg cgggctcgac aaatcgggca tccgcctgtt cggggcgggc gacatcaccg
      421 acgacgacct cctcaacaac atgggcgacg tcgcgctggg catcgagacc gcatactttt
      481 attccgccgc ccatccctcc gagaagaaca aggcattcgt cgagggggtg aagcgggcga
      541 acaacggcat gcgcgcgaat tttttcggcg tgtcgggcta cgacggcatg catctcatct
      601 gcgaagcgat ccgaaaaaca ggcggcaaga ccgacggcga cagtttcatc ggcgcggtga
      661 aagggatgtc gtgggaaagc ccgcgcgggc cgatgaccat cgacccggaa acgcgcgacg
      721 tcattcacaa cgtctatatg cgcagggtcg aaaaggtgaa cggcgagctc tggaacatcg
      781 agttcgcgac cgtggagaat gtcaaagacc cggtcaaggc ggcgcggaaa tagcgtgcgg
      841 tggagttggt gccatggcgg gacggcgggg cggccgagcg acatgagcac gacgcagcaa
      901 actccgctcc tgccactgcg agcgggtcgt tacaatcagt gagaggagac gcggccatgc
      961 agagcaagga cgaagaggtg accccgcgct atttcaatcc gacgtgggcg ccaacgcttt
     1021 cggatgaggc gcgcaaggcg gtgaacgcgg cctttgacgc gatgtccgcc tggcgcatcg
     1081 agaccgccag caacaacgag aaaaacagcg agcaggtgat cgagaagctg gctgccgccg
     1141 cgcgggcgct gggctggccg gagcaaatcg tcgatgccac ccgcgcgcaa atgcagggca
     1201 tcaccaaaat gcagatccag acaatggatc agatgatgga cgcttgggag gagcagatca
     1261 gatcacccaa cgcgatgacc ggctcgccgt cggcgatgct ctcgaagcta aagaccttgc
     1321 ccggcttcag cccggccggc agttggccaa gcgcagacgc gttggcggcg acgaacccca
     1381 tggagttttg gatgcaatgg gcggagcagt ggcagaaagc gtgcacagac gcgatggcgc
     1441 tttgggacaa ggccggtcag ccgggttatg gcgtgggacc gcgccgccgc tgaacctcgg
     1501 aactgtgtgg agcgaggtga cgtcatgcgc ctcttcgccg ttgcgctcac gctatcgctc
     1561 gccgccgcca cggccgcatc cgcgcagacg gcgacgccgg atagcgaaaa tggccgctat
     1621 agcttcaatc ccgtggccga cggcgtgctg cggctcgaca cccgcaccgg ccaggtctcg
     1681 cagtgcagcc gcagcgatgc cggctgggcc tgcaaagtgg tgcccgacga gcgctcggcg
     1741 ctggagaccg agatcgtccg cctgcagggc gagaatgcga cgatgaagaa ggagctcttg
     1801 tcgcgtgggc tgccggtgcc cggcgtttcg gctccgtcag gcgcaaagcc cggcgaggga
     1861 gagctcaagc tgccgagcga tgccgagatc gacaaggtgg tcgcgttcct cgagaaggtt
     1921 tggcgccgtt tgatcgagat gggcaagaac gtgcagaagg acgtcgagaa gaaaaattga
     1981 aggagctggc gggcgtgacc atcggcggtg ccggcatcgc cgcgatgaga cgggtcaggt
     2041 gttgcgtccg tccaacattt ttccaaccgc gtaaggctcc cgtaagggag cgcgtataga
     2101 ctcgagcgtc gtcaaaaccg tcactcattt agcgacgatg ccccggcggg agcccactgc
     2161 cggggcattt ttttaagtaa gtcgcgctga tcgcgaatgg attggaggac acattcatgt
     2221 ggtcggcact ggccggccgc tcgcggctcg ccccgaactc ttcaccaacc ctcgcttttg
     2281 gccgacggtg ccactgggat ggaccttccg aagccgattg ccgcgggcga attcatttgc
     2341 tctgttctcg atcgcgccac aaactgatgg ccttcgctcg tagaactcgg tagcagtatt
     2401 cacggcgaac taaatacgta acgcgataat gtttgtttcc ggcaagtacg tgaatactct
     2461 ggaacctgac gacctgaaga aaaggaatcg cgcctgggca gcgcgtatga cctcggagga
     2521 tcctgacttc ttccggcgcc tcgaacgcca acaggcgccc cattatctct ggatcggctg
     2581 ttccgatagc cgcgtgcccg caaatacgat tgtcggactc gaccccggcg agttgttcgt
     2641 ccaccgcaat gtggccaatc tcgcttcgcc gcaggatgcc aactatttat cagtgctcca
     2701 gtttgccgtc gacgtactca gagtgcagca catcatggtg gtcggacatt atggctgcgg
     2761 cggtgtcagt gccgccgtcg atggcagacg tcgaggtctt gtcgaccatt ggctgcatcc
     2821 aattcgcgag ctgtccagaa ggcacagcga ggaactcgaa gcgctgcggg acgagcacag
     2881 ccgtctggac cgtctgtgcg aactcaatgt ggtgcagcaa gtcaagaacg tggcctccga
     2941 cgtcttcgtg ctcgacgcct gggcacgcgg gcagaatctc tcggttcatg gctgggtgta
     3001 ttcgatcggg aatggtctga tcaaagattt gaacgtgacc gtgagcgctc cggggcaatg
     3061 ctgatggcgg cggccatggg ggcctcgccg atcgcttgtt ctaaggtggt gcgcaagatc
     3121 ggcctgtcgt ggatgcgttc gctgtggacg gcgcccgcga agacgggcgt gaacgccctt
     3181 aacgacgcgc tatggcgaaa tccgggatat cacgcaacgc gctgaacgct gtccccggaa
     3241 ccgtcagcat ttccgcccct ggattgcgcg aacaatgccc catttgcgcc aatgcggcgc
     3301 cattgtggcg gcgcggtata cgtccatcca cctctgggga caggaggatg caatgctcac
     3361 gctacgtact ttggcaatcg taatagctgg ggttggggtg ttcgcgggtc tgccattgac
     3421 gcctgcgcag gcgtgcgatg acgatcgcta tccctgcccc ataagggtgc cacaagaggt
     3481 cgatgcgccg gctcaggcca cgccgtcggt gcagccgcaa aagaaagcga gccacgctgc
     3541 gcgcccaagt gagaaggcgc aagcaaaacg cgaggcgcca cccgccgccg cccgcgcgaa
     3601 gccgagcaaa ccggctgcgc cggagcaggc ggccgatccc atcgcgcaga aggcggcaga
     3661 ggctcctccc gcgctggtgc cgtcgccgcc cgcggatcaa cctctcaatg ccgcaagccg
     3721 gggcgaaagt ccggtggcgg ctgcggggac ggcttggccg gttctgccaa cgaccgagga
     3781 cgcgggcgct agcgcgccag gagccgcgcc tgcggcgacg agcgcggatg cgacggaagc
     3841 cgccaacacg actgcggtga cgaccgcggt gacaaatgc
//
LOCUS       DATLJT010001538         1870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3132615,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001538 DATLJT010000000
VERSION     DATLJT010001538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132615"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VM910_35195"
     CDS             <1..190
                     /locus_tag="VM910_35195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE/phosphotransferase"
                     /protein_id="HVI67791.1"
                     /translation="IMSAQRNTRLLGTFARLNRRDGKPQYLKHLPRIWTYLSRSLAHP
                     ELDPVRAWYEAHVPAPAS"
     gene            288..593
                     /locus_tag="VM910_35200"
     CDS             288..593
                     /locus_tag="VM910_35200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVI67792.1"
                     /translation="MASKGDRVTFERGIPEHMMGIDGTWRRDCTMEDVSETGAKLTIE
                     GSVEGLHLKEFFLLLSSTGLAYRRCELSWVNGDQIGVTFLKQGDRKRKAGRRAPEEA"
     gene            724..1446
                     /locus_tag="VM910_35205"
     CDS             724..1446
                     /locus_tag="VM910_35205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVI67793.1"
                     /translation="MPVSPTKAMVLAAGLGLRMRPLTDHLPKPLVRIAGRPLLDHVLD
                     KLAAAGVAEAVVNVHYLPDQIIDHVASRKKPHVIISDERNQVLGTGGGVVKALPLLGP
                     APFFHVNSDTMWIDGVQSNLARLAHVFDPARMDILLLMAPTASSIGYSGRGDYAMLPD
                     GALRKRREHQVVPFVYAGAAILSPKIFAEAPKGEFSLTKMFDAANEQERLFGLRLDGV
                     WMHVGTPDAIAAAEEAFLESVA"
     gene            1633..>1870
                     /locus_tag="VM910_35210"
     CDS             1633..>1870
                     /locus_tag="VM910_35210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67794.1"
                     /translation="MRVFSVPLSAPFLHSVIAALIDGRLVEGFEARTNPAKLADATLY
                     LPTRRAVRVAREVFLEELKTDAAVLPRITPLGDID"
BASE COUNT          336 a          621 c          571 g          342 t
ORIGIN      
        1 catcatgtcg gcacagcgca acacgcggct gctcggcacc ttcgcgcggc tgaaccgccg
       61 cgacggcaag ccgcaatatc tcaagcacct gccccggatc tggacctatc tcagtcgttc
      121 gctggcgcat ccggagctgg acccggtgcg ggcctggtat gaggcccatg tgcccgcacc
      181 cgcttcctga aaggacgaat ttaccgcctg ttagtcgacc gcccattagt ctgcgggagg
      241 ccgcgctgcc gacgcaccgg ccatctgcga gaggctggag cgaagacatg gcgagcaagg
      301 gcgaccgcgt caccttcgag cgcgggattc ctgagcatat gatgggaatc gacggcacct
      361 ggcggcgcga ttgcacaatg gaggacgtgt cggagaccgg cgcgaaactc actatcgagg
      421 gatcggtcga aggcctgcac ctgaaggaat ttttcctgct cctgtcgtcc accgggctcg
      481 cctatcgccg ttgcgagttg tcctgggtca atggcgacca gatcggcgtc accttcctca
      541 agcagggcga caggaagcgg aaggcgggac gccgcgcgcc ggaggaggcc tgaaacctgc
      601 cggatccccg cgcgtcgcac agccgtcatc cgacaccgct atggcgtcca ggccatgcgt
      661 ccgccgaatc gccgtgatat gattcgcggc aagcagaaga caggttcgag acagttttgg
      721 aaaatgcccg tcagccccac taaagccatg gttcttgccg ccggcctcgg cctgcgcatg
      781 cgtcccctga ccgaccattt gccgaaacct cttgtgcgca tcgcaggccg ccccctcctc
      841 gaccacgtcc ttgacaagct cgccgccgca ggcgtcgccg aagccgtggt caacgtgcat
      901 tacctgcccg accagatcat cgatcacgtg gcctctcgca aaaagccgca tgtcatcatt
      961 tcggacgagc gcaaccaggt gctcggaacc ggcggcggtg tggtcaaggc cctgccgctg
     1021 ctgggcccgg cgccgttctt tcacgtcaac tcggacacga tgtggatcga cggcgtgcag
     1081 tccaatcttg cacggcttgc gcacgtcttc gatcccgcgc gcatggacat ccttttgctg
     1141 atggcgccga ccgcgagcag catcggctat tccggccgcg gcgattacgc catgctgcct
     1201 gatggcgcgc ttcgcaagcg gcgcgaacat caggtggtgc cgttcgtcta tgcgggcgcg
     1261 gcgatccttt cgccgaaaat cttcgcggaa gcgccaaagg gcgaattttc cttgacgaaa
     1321 atgttcgacg ccgccaacga gcaggagcgg ctgttcgggc tcaggctcga cggcgtctgg
     1381 atgcatgtcg gcacgccgga cgcgattgcc gccgcggaag aggcgttcct ggaaagcgtg
     1441 gcgtagcgcc ccctgtcgtc ccggccaagc ggcaggctcg cgacccataa cgtgcttaag
     1501 tggctgggtc accattttcc caacaggacg acattgtgga ttgtgggatg cgtgcgcaac
     1561 ccgaccctgc cccatgacca acctccaatc gctccctata ttggcgcctg actcttccga
     1621 atcaggcagc ccatgcgcgt tttcagtgtc cctctctccg cgccgttcct gcacagcgtg
     1681 atcgccgcgt tgatcgacgg cagattggtc gaaggatttg aagcccgcac caatcccgca
     1741 aaacttgccg atgcgacgct ctatctgccg acgcggcgcg cggttcgcgt cgctcgcgag
     1801 gtgttcctcg aggaattgaa gacggatgca gccgtgttgc cgcgcatcac gccgctcggc
     1861 gacatcgacg
//
LOCUS       DATLJT010001539         1556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4280525,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001539 DATLJT010000000
VERSION     DATLJT010001539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4280525"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VM910_35215"
     CDS             <1..316
                     /locus_tag="VM910_35215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI67795.1"
                     /translation="GISPLFVTQADKLVALAARHTMPVMYFRREFAAAGGLVSYGSGT
                     AEYYRQLGVYAGRILKGEKPADLPVVQSTKFELVINLKTAKAFGMTIPPGVLAIADEV
                     IE"
     gene            406..1293
                     /locus_tag="VM910_35220"
     CDS             406..1293
                     /locus_tag="VM910_35220"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67796.1"
                     /translation="MRTIGILASQPLPPIQRFTRKLQDYGYVQGQNLRLVSRFAEGRD
                     DRYPAMAVELAALPVDLIVTWGTPAALAAKQATRTIPVVMGAIGDPVSVGIVSNLARP
                     DGNITGFAAQNVDLEGKRLELLKDFLPHLSRVGILANSGNPLFDVSLQRLRPAAEQLR
                     VAVDVFDIHGSGGIEAALLGLDRTRPDGVLVAPDLVLLSQRRQIAAALANSKLPAVYP
                     FREYIDAGGLIIHGANLGILFERAAGYVDRILKGAKPADLPVQLATEFELIINLKTAS
                     IIGVTIPPTLIARADEVIE"
     gene            1290..>1556
                     /locus_tag="VM910_35225"
     CDS             1290..>1556
                     /locus_tag="VM910_35225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67797.1"
                     /translation="MRRREFIALLGAAAARPLGARAQQPGKLPTIGFLGSTTPAAQSQ
                     LTAAFVQRLRDLGWIEGRTVTIEYRWAEGRSERLAEFAAEFVRLK"
BASE COUNT          277 a          475 c          489 g          315 t
ORIGIN      
        1 cggcatcagt ccgcttttcg tcacccaagc ggataaactc gttgcgctgg cggcccgcca
       61 tacgatgccc gttatgtact ttcggcgcga gttcgccgcg gccggcggac tggtgagcta
      121 cggatcgggc actgcggaat actaccgcca actcggcgtt tacgccggcc gcattctcaa
      181 gggtgagaaa ccggccgatc tgccagtggt gcagtcgacc aaattcgagc tggtcatcaa
      241 ccttaagacc gccaaggcgt tcggcatgac aatcccgccg ggcgtactcg caatcgccga
      301 cgaggtgatc gaatgaggcg ccgcgagttc atcgcgctgc tgggcgccgc ggcggcgcct
      361 tcgctgcttt ggcagcgcgc ggcgcgcgcg cagcaggctg aacgggtgcg gaccatcgga
      421 atccttgcgt cgcaaccctt gccgccgatt cagaggttta cgcgcaagct gcaagactac
      481 ggctatgtcc aaggccagaa tctgcggttg gtatctcgct ttgcagaagg tcgtgacgac
      541 cgctaccccg ccatggcggt cgaattggct gcgcttccgg ttgatctgat cgtcacatgg
      601 ggcactccgg ctgccttggc ggcgaagcaa gcaacgagaa cgattcctgt cgtgatgggt
      661 gccattggtg atccggtcag cgtcggtatc gtctcgaacc tcgcgcgccc cgatggaaat
      721 attaccggct tcgccgctca gaacgtcgat ttggagggca agagactgga gctgctgaaa
      781 gactttcttc cgcatctctc tcgcgttggc atcctggcga attctggcaa cccgttgttc
      841 gatgtttcat tgcaaagact gcggcccgcc gctgagcaat tgcgcgtagc ggtcgatgtg
      901 ttcgacatcc acggtagcgg tggcattgag gccgctttgc tgggattgga ccggacgcgt
      961 ccggacggcg ttctggtcgc gcccgacctt gtcctacttt cccaacgaag gcagatcgcg
     1021 gctgccctgg cgaacagcaa gcttccggct gtctacccgt ttcgagaata tatcgatgct
     1081 gggggcctca taatccacgg tgcgaatctg ggcatcttgt ttgagcgggc agcgggctat
     1141 gtcgatagaa ttctgaaggg cgccaagcct gccgatctgc cggtgcagct ggcaaccgag
     1201 ttcgagctaa tcatcaatct gaagactgcc tccatcattg gcgtgactat tccgccgacg
     1261 ctcatcgccc gcgccgacga ggtgatcgaa tgagacgccg cgagttcatc gcgctgcttg
     1321 gcgccgcggc cgcgcggccg cttggcgcgc gcgcgcagca gccggggaaa ctgccgacca
     1381 tcggattctt gggctcgact acgcctgccg cgcaaagcca attgaccgct gcttttgtgc
     1441 agcggctgcg cgacctcggc tggatcgaag gacgcaccgt cacgatcgaa tatcgctggg
     1501 cggagggacg cagcgaacgc ctcgctgagt tcgcagccga gttcgtccgg ctgaaa
//
LOCUS       DATLJT010001540         1636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2442943,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001540 DATLJT010000000
VERSION     DATLJT010001540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442943"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VM910_35230"
     CDS             <1..607
                     /locus_tag="VM910_35230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HVI67798.1"
                     /translation="RLARFGTANEHADWETAHHVFTYANAIHQMLKRIGTADRDGHIE
                     AVHGVLHGAMALYLARYLNVPPARIPGEGSEPLDDLPANAEEIRVALLDVFDRQRQVD
                     LAARLVARHLTLGHSVQSLIAILARALLREDAGFHAYQVLDAGVRQFGEWGNTDEGRH
                     ILIAVVRFLAAHSPTERAELQTADIAQRLMRGGELHQDTTA"
     gene            complement(767..1090)
                     /locus_tag="VM910_35235"
     CDS             complement(767..1090)
                     /locus_tag="VM910_35235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67799.1"
                     /translation="MVKTPLAFEFSKTRTICDAFNDAWALLQGLGSDLTQAPKSLATQ
                     TILARRIIEMADQGLRDIPALRDDALAFLQHSTPSASRNGLGEAIKVQAAALAELENK
                     AIETA"
     gene            complement(1259..1450)
                     /locus_tag="VM910_35240"
     CDS             complement(1259..1450)
                     /locus_tag="VM910_35240"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI67800.1"
                     /translation="MRFGRPPKLNAHQPRRALARREKGETLMDLARSYGVSRREQCPA
                     RLQLAGILHPSGAFWHRSA"
BASE COUNT          312 a          494 c          498 g          332 t
ORIGIN      
        1 gaggttggca cgcttcggga ccgccaacga gcacgccgat tgggagacgg cccaccacgt
       61 tttcacttac gccaatgcca tccaccagat gttaaagcga attggcacgg ccgaccggga
      121 tggccacatc gaggcggtgc atggggttct acacggagcg atggccctct accttgctcg
      181 ctatctcaat gtgccgccag cccgcattcc cggcgaaggc agcgagccgc tcgatgacct
      241 gcccgccaat gcggaggaga tccgcgttgc tctgctcgat gtcttcgatc ggcaacgaca
      301 ggtcgatctc gccgcgcgtc tcgtcgcgcg gcatctcaca cttggtcact cggtgcagtc
      361 gctgatcgcc atcctggcgc gagcgctatt gcgcgaggat gcaggttttc atgcctacca
      421 ggtgctggac gccggcgtgc ggcagttcgg cgagtggggc aataccgacg agggccggca
      481 tatcctcatc gcggtcgtcc gctttctcgc tgcgcattcg cctacggaac gcgccgaact
      541 gcaaacggcc gacatcgcac agcggctcat gcgtggcggt gagcttcatc aggataccac
      601 agcctgaacg ttggacagcg tcgcttacgt aattttggct ctccagggaa aacgtccaaa
      661 acgctcggcg gtggtggcgg gcgttttcga ttcagccctt tcgaaattgt gaaggaccca
      721 ctcgttccgc cgcgtggaaa gggacccggc gaggccactc actcgctcaa gctgtctcaa
      781 tcgctttgtt ctctagctcg gcgagcgccg ccgcctggac tttgatggcc tccccgagcc
      841 cattgcgact agctgacggc gtactgtgct gaaggaaggc taaggcgtca tcacggagtg
      901 ctggaatgtc tctcagcccc tgatcggcca tctctatgat gcgcctggcc aggatcgttt
      961 gcgtcgctag cgatttgggt gcttgcgtga ggtcgctgcc aagaccctgc aagagcgccc
     1021 aggcatcatt gaacgcatcg caaatcgtac gtgtcttgct aaactcaaac gccagcggag
     1081 ttttgaccat gacgcgcccg acatgctgcc ggtgacagag cggacggacg ctcaaaaggg
     1141 tcccgtgatg aagtggattg caggcccggt cgctttgcga cagcggctcg atcacttgga
     1201 ggctaggccc gccacagtcg gaagtcgagc tacagtcttg tgacgatccg caaattgctc
     1261 acgcgctgcg gtgccaaaat gcccctgacg ggtgcagaat accggcgagt tgtagtctcg
     1321 cggggcattg ctcgcgccta ctgactccgt agctccgagc gaggtccatc aaggtttcgc
     1381 ccttctcgcg ccgcgccagg gccctccgag gttgatgggc gttgagcttt ggcggacgtc
     1441 caaagcgcac atcatagcgg cccgcacatc tgcgccacgt agccctggtt ctttgagaaa
     1501 gccgtgcgcc aagaacgtcg cccagccgta aagggaagat aacattttgg cgtgatctgc
     1561 cgaagagagg ggcatttctt aacttccccg atagaaactt agatttcggg aggggaagga
     1621 gtgctgttat ggaacc
//
LOCUS       DATLJT010001541         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1984369,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001541 DATLJT010000000
VERSION     DATLJT010001541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984369"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VM910_35245"
     CDS             <1..395
                     /locus_tag="VM910_35245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HVI67801.1"
                     /translation="VRFAGAAYLVWLGIKLIRAPVEGLSADELPPPRGGFFLQGFLVA
                     LSNPKLLLFFGAFIPQFMDMSKDHLSQVLLLGVTFMVLAGLTDATYALLAGRARKFFS
                     ARRTRLVSRISGGCMIGGGIWLALVRAR"
     gene            complement(431..1234)
                     /locus_tag="VM910_35250"
     CDS             complement(431..1234)
                     /locus_tag="VM910_35250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HVI67802.1"
                     /translation="MDSRPARATSLLLDSDEVSPVFEQNAGSRSPFLFASDHFGRLIP
                     NRLGNLGLPASELGRHIAFDVGIAGVAERLSFLLGAHLIAQRYSRLVIDCNRPPDVAS
                     SIPLISDATAIPGNEGLARDAAETRRREIFDPYHRHITAVIDRRLREAAPTVLVALHS
                     FTPVYDGVVRPWHIGTLYHRDTTLPPLLLSALRAEADLVVGDNEPYAVSDTTDYTIPV
                     HGEARGLINTGIEIRQDLIADETGQQQWAERLARILRGIETTLRRERLI"
     gene            1339..2241
                     /locus_tag="VM910_35255"
     CDS             1339..2241
                     /locus_tag="VM910_35255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI67803.1"
                     /translation="MPRLRIHHKTEYRYARPVAFGEHRVMLRPRDGHDLRVLDGRLDI
                     SPEPMRLRWIHDVFGNSVAIATFDERATRLTFVSEVTVEHNPADEFALTPDDHAYFYP
                     FLYDAEEYPDLQQYVTPHYADPNGELSAWARNFLDAEAPTPTFKILSGITHGIRESFV
                     YRKRHEQGTQHPLDTLQTGSGTCRDYALLMIEALRRLGIAARFVSGYLFTGDDARHVG
                     GGSTHAWVQVYLPSAGWIEFDPTNGIVGTRDLVRVAVARDPRQAIPLHGTYLGRKSAF
                     IDMKVNINVVSLDEEQELETEAGA"
     gene            2245..>2918
                     /locus_tag="VM910_35260"
     CDS             2245..>2918
                     /locus_tag="VM910_35260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI67804.1"
                     /translation="MDIKVGFEITYAAEQATPMVIMLSIHPSRLSDIVGGETTIAEPN
                     VPIGFYRDSFGNICGRLVAPRGGVTLRGNALVRDSGLPDVVNLAAQQLPIDQLPDELL
                     LYLMPSRYCETDKLTDTAWSLFGKTKPGWERVQAINDYVNEHVTFGYQHAHHMKSAHD
                     VYGQRNGVCRDFAHLALTFCRCMGIPARYCTGYLGDIGVPPEPEPMDFSGWHEVYLSG
                     QWYTFDA"
BASE COUNT          502 a          930 c          934 g          552 t
ORIGIN      
        1 gggtgcgctt tgcgggcgcc gcctatctgg tctggctcgg catcaagctg atccgcgcgc
       61 cggtcgaggg tttgagcgcc gacgagctgc cgccgccgcg cggcggcttc ttcctgcagg
      121 gctttctggt cgcgctgtcg aacccgaaac tgctgttatt tttcggtgcg ttcatcccgc
      181 agttcatgga catgagcaaa gatcacctct cgcaggtgct gctgcttggc gtcaccttca
      241 tggtgcttgc gggattgact gatgcgacct atgcgctgct tgcgggccgg gcgcggaaat
      301 tcttttcggc gcggcgcacg cggctggtgt cgcgcatctc gggcggctgc atgatcggcg
      361 gcggcatctg gctcgcgctg gtgcgggcgc gttagttaga ccatgatctt cgcgcaaagc
      421 ggatcatgcg ctagatcaac cgctcgcgcc gcagcgtcgt ttcgatcccg cgaagaatcc
      481 gcgcgagccg ttccgcccat tgctgctggc cggtctcgtc ggcaatcaaa tcctggcgga
      541 tctcgatgcc tgtgttgatg agcccgcgcg cttcgccgtg gacgggaatg gtgtagtcgg
      601 tcgtatcgct caccgcgtag ggctcgttgt cgccgaccac gagatcggcc tcggcccgca
      661 gcgcgctgag caacaaaggc ggcagcgtcg tgtcgcgatg gtagagcgtg ccgatgtgcc
      721 agggccgcac gacgccgtca taaacgggcg tgaagctgtg cagcgcgacc agcacggtcg
      781 gcgcggcctc gcgcaggcgg cggtcgatca ctgcagtgat atggcgatga tagggatcga
      841 aaatctcgcg ccgccgcgtc tccgcggcgt cgcgcgcgag gccctcattg ccgggaatgg
      901 cagtggcgtc actgatcagc gggatcgagc ttgcgacgtc cggcgggcgg ttgcagtcga
      961 tgaccagccg cgagtagcgc tgcgcgatca gatgcgcgcc cagaaggaaa gacagccgct
     1021 cggcgacgcc ggcgatcccg acgtcgaagg cgatgtggcg cccaagctcg cttgccggta
     1081 gcccgagatt gccgagccga ttcgggatca gccggccgaa atggtcgctg gcgaacagga
     1141 acggcgaacg gctacccgca ttttgctcga ataccggcga tacctcgtcg ctgtccagga
     1201 gcaaggatgt tgcgcgtgca ggccgggaat ccatatttcg ttgcctatcg aagcggcatg
     1261 tcgatctaga aatgagcagc gatctctata gattagacgc ccgggaatca cgacaataat
     1321 cacaaagatt gtgtgacgat gccgcgcttg aggattcatc acaagaccga atatcgctat
     1381 gcgcgcccgg tggcgttcgg cgagcatcgc gtgatgctgc gcccgcgcga cggccatgat
     1441 ctgcgcgtgc tcgacggccg gctcgacatc tcgcccgagc cgatgcggtt gcgctggatt
     1501 cacgacgtgt tcggcaactc ggttgcgatc gcgacctttg acgagcgcgc gacgcgcctc
     1561 accttcgttt ccgaggtgac cgtcgagcac aaccccgcgg acgagtttgc gctcacgccg
     1621 gacgaccatg cctatttcta tccgttcctc tacgatgccg aagaatatcc ggatctgcag
     1681 caatacgtca cgccgcacta cgccgacccc aacggcgagc tttccgcatg ggcgcgcaat
     1741 ttcctcgacg cggaggcgcc gaccccgacg ttcaagatcc tgagcggcat aacgcacggc
     1801 atccgcgagt ccttcgttta tcgcaagcgc catgagcagg gcacgcagca tccgctcgac
     1861 acgctgcaga cgggatcggg cacctgccgc gactatgcgc tgctgatgat cgaggcgctg
     1921 cgccggctcg gcatcgccgc gcgcttcgtc tcgggctatc tgttcaccgg cgacgatgcc
     1981 cgccatgtcg gcggcggctc gacgcatgcg tgggtgcagg tctacctgcc gagcgccggc
     2041 tggatcgagt tcgatccgac caacggcatc gtcggcacgc gcgatctcgt tcgcgtcgca
     2101 gtcgcccgcg acccgcgcca ggcgatcccg ctgcatggca cctatctcgg tcggaagtcg
     2161 gccttcatcg acatgaaagt gaacataaat gttgtttcgc tggacgaaga gcaggagctg
     2221 gaaacggagg ctggagctta agagatggac atcaaagtcg gcttcgagat tacctacgct
     2281 gccgagcagg cgacgccgat ggtgatcatg ctgtcgatcc atccctcgcg cctttccgac
     2341 atcgtcggcg gcgaaaccac catcgccgag ccgaacgtgc cgatcggctt ctatcgcgac
     2401 agctttggca atatctgcgg acgcctggtg gcccctcgcg gcggcgtgac gttgcgcggc
     2461 aacgcgctgg tgcgcgattc aggcctgccc gacgtcgtga atttggccgc gcagcagttg
     2521 ccgatcgatc agttgccgga cgaactcctg ctttatctga tgccgagccg ttattgcgag
     2581 accgacaagc tcaccgacac cgcctggtcc ctgttcggca aaaccaagcc cggctgggag
     2641 cgcgtgcagg ccatcaacga ctacgtgaac gagcacgtca ctttcggcta tcagcacgcc
     2701 catcacatga agtcggcgca cgacgtctac ggtcagcgca acggcgtgtg ccgcgacttc
     2761 gcgcatctcg cgctcacctt ctgccgctgc atgggaatcc cggcgcgcta ctgcaccggc
     2821 tatcttggcg acatcggcgt gccgccggag ccggagccga tggatttctc cggctggcac
     2881 gaggtctatc tctcgggcca gtggtacacc ttcgatgc
//
LOCUS       DATLJT010001542         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_144644,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001542 DATLJT010000000
VERSION     DATLJT010001542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144644"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VM910_35265"
     CDS             <1..233
                     /locus_tag="VM910_35265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67805.1"
                     /translation="EGFGAGGATDIVARLTGQALSERLGQSFVVENRAGASSNIATEA
                     VVRASPDGYTARRCRPTSSPRSPARFRSEPDR"
     gene            319..483
                     /locus_tag="VM910_35270"
     CDS             319..483
                     /locus_tag="VM910_35270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67806.1"
                     /translation="MAGEMFKMMAGVDLFHVPYRGYIRAGNLRALAVTTTTRSQILPD
                     VPVRFRRTLN"
     gene            complement(475..900)
                     /locus_tag="VM910_35275"
     CDS             complement(475..900)
                     /locus_tag="VM910_35275"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67807.1"
                     /translation="MRGFIYSNMLQRTLPAGFIAPCLPTKTDKLPSGSQWLHEIKHDG
                     FRIIARKDGPRVRLYSRPGNDLTHRFPLIVETLARLRSRSCIIDGEAVACDDNGVASF
                     NLIRYRRHDDSTFLYAFDLIELNGDDLQRTPRPILARSI"
     gene            complement(1794..2006)
                     /locus_tag="VM910_35280"
     CDS             complement(1794..2006)
                     /locus_tag="VM910_35280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVI67808.1"
                     /translation="MPSGRVKFFNTDRGYGFIAPDDGSSDVFVHVHDVEVAGMKILEA
                     GQMVAYEVGPARDGRSKAVNLRLLKP"
BASE COUNT          437 a          662 c          674 g          440 t
ORIGIN      
        1 tcgaaggctt cggcgccggc ggagcaaccg acatcgtcgc gcgattgacc gggcaggcgc
       61 tgtcggagcg acttgggcag tcattcgtgg tcgagaaccg agccggcgcc agcagcaata
      121 tcgccaccga ggcagtcgtg cgggcatccc cagacggtta cacagcgcgc cgatgccggc
      181 ccaccagctc gcctcgtagc ccggcacggt ttcgctcaga acccgatcga tgagagcagc
      241 gggccaaaat atacctgcgc ctcgccggcg agcagaggcc cgcgatcgga aggtgaggcc
      301 gccaaactga gcctcgccat ggccggtgaa atgttcaaga tgatggccgg cgtcgatctg
      361 tttcatgtgc cttatcgagg gtatatccgg gccggcaacc tgcgcgcctt ggcggtaacc
      421 acgacgacgc gctcgcaaat cctgccggac gttcctgtcc gattccggcg gacgctaaat
      481 tgatcttgcc aggattggcc ttggcgtacg ctgcagatcg tcgccattca gttcgatcag
      541 gtcgaaggcg tacaggaacg tgctgtcatc atggcggcgg tagcgaatga gattgaacga
      601 ggcaacgccg ttgtcatcgc aggcgacagc ctcgccatcg ataatgcaag agcgcgagcg
      661 caggcgcgcc aacgtctcga cgatcagcgg gaagcgatgg gtgaggtcgt tgcccggacg
      721 gctgtagagc cgcacgcgcg gaccgtcctt gcgggcaatg atacggaagc cgtcgtgctt
      781 gatttcgtgc agccattggc tgccggacgg cagcttatcg gttttggtcg ggaggcacgg
      841 cgcgatgaac ccggcgggga gggtacgctg caacatgttg gaataaataa agccgcgcaa
      901 tgtttgttgc aactatagcg gccagccatg ggtctagtca gttggtcatg agaggagcgc
      961 gcattatgtt tgatgagttt gacggcctgg acgcgaccga aaaggctggc attgggaccg
     1021 cgctgaatag gtgtcgcgcc ctcgcggcca aaattgcgaa tgaactccgt aacaagaggc
     1081 ccgagcactt cgttaacaag ttgtcactgg cagacgttca cgcctccctt gagcacgctg
     1141 ccgatgtcct cagcacagtg aagggctcgc gggcgcggcg ccgtcagcgg gatcgataat
     1201 tgaaagcgat cttcgtctcc ccacgctctc gcgggcgtgg tgcggccgtg ccgccgggtg
     1261 atccgggaaa agtcgcaatt tcgtgctcaa catgattcgc tgtcattgag tccccactca
     1321 tgacggcacc agcctttatc taaacttccg agtcctggcc gccctcccct ttgggcggcc
     1381 ttttttcgcc ccagcgctca cgcttgagcg aatgagtgcg ggttatttgc cccagtcctc
     1441 ctcctcttcc cgcttcacgg ccggtgcatc cgagttcttc attttgagcc aatcgggcga
     1501 gcgaccggaa cggtagccgg agcccttgcg cttcgagacg atgccttcga ggccaagctt
     1561 gcaggcatgg gcgaagacgg tcgggccgtt gcccccgaac cggtgaccta gtattgcaac
     1621 gcaagcgtgc ggattgtggg accgctagcc ctgtcattgc cgctctctgc taagttctgc
     1681 agcaaaatct tgtgcgagat cgagcgagcg acgaaattgc acaacgccca cgcgtccaat
     1741 tccggtggtc gattgaggaa catccgcatc gcaggagtga ggcaattcta acgctatggt
     1801 ttaagcaaac gaagattgac ggcttttgag cggccatccc gcgcgggacc gacttcgtat
     1861 gcgaccattt ggcctgcttc cagtatcttc atgccggcga cctctacgtc gtggacatgc
     1921 acaaaaacgt cggagctgcc gtcatcgggc gcgataaagc catatccgcg atctgtgttg
     1981 aaaaatttga cccgtccact tggcatgtcc aattaatccg atctcaacgc tcagcacccc
     2041 cgccgtcggt acgacggggc cggggcttcg cacaacgcgc aacctcagcg aggcgcgtca
     2101 tgcgagatgg gccaaatttt cctgtccttg tggtacaccg cgtagttccc gccaagctgg
     2161 cgacccgcaa atctgacggc ttcgtcctta gtggaaaacg ctcgcaccgg ctc
//
LOCUS       DATLJT010001543         3493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_607293,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001543 DATLJT010000000
VERSION     DATLJT010001543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607293"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VM910_35285"
     CDS             <1..1110
                     /locus_tag="VM910_35285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011992963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVI67809.1"
                     /translation="ALGLATPMAVMVGTGLGAMNGILFKNAGALEEATKLDVIIFDKT
                     GTLTVGQPEVVDVVISRRVATDTVLMTAAAVEQGSDHPLAQAIVRRAQGLAVPVQKGF
                     ENIEGMGARAEIDGEPVFLGNRRLMDTQKLDLGELAAEAQRLQGAGRTVVHVARAGKV
                     IGLIAIADAPRSRAAATVAKLRERGVQVAMLTGDNAGTAHRIARDLGLDIVLADVLPG
                     QKAGKVKELQAQGKKVGMVGDGVNDAPALTQADVGFAIGAGTDVAIESADVVLMKSDP
                     YDVVGAVELSHATLRKMHQNLWWAVGYNVIAFPLAAGVFYPFLLSPEIAALAMSGSSA
                     LVAINALMLKRTKLAGITRRSSVQTSPLRAAEVTA"
     gene            1107..1736
                     /locus_tag="VM910_35290"
     CDS             1107..1736
                     /locus_tag="VM910_35290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67810.1"
                     /translation="MSEELGRQLLAGRALFALLLGMFAITVGCGIVLPILRFLIERLT
                     ATADAAILSWHTGLLTGTYILAIFLFTPPWDMSETEHGEESSIRSLMPLFRYYAIVLS
                     LSLADSRRINPYSSAPIFFSSSHAAAAVIPPTMPMTKIGMSVLRIQCQSTPAMKLVRS
                     TVATVISNLCDGDTAFSPCASQCAPDGIFANDTARHERPPQTEKGISYP"
     gene            complement(1723..2484)
                     /locus_tag="VM910_35295"
     CDS             complement(1723..2484)
                     /locus_tag="VM910_35295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HVI67811.1"
                     /translation="MVAEASVTIYRRDFDAAIFDLDGVLTDTAGIHAAAWKAVFDAFL
                     RKRAERDGGSFRPFDVAADYLAYVDGRPRYDGVRNFLAARNIVVPEGSEHDPEDAETV
                     RAIGERKTRLFRQALQQGIEPASGAEALLKELRRVGIGIAVGSSSENCAAILRAARLD
                     RLIDVRVDGIDAEKLGLPGKPDPALFLEAARRLRVEPSRAVLFEDALAGVEAGRRGNF
                     GCVIGIDRGRQPDALRRHGADVVVESLLQVEVMDS"
     gene            complement(2777..3241)
                     /locus_tag="VM910_35300"
     CDS             complement(2777..3241)
                     /locus_tag="VM910_35300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI67812.1"
                     /translation="MLVEAAVTINRRDFDAAIFDLDGVLTDMAGVHAAAWKTVFDAFL
                     QRWAEQHGRAFQPFDIHSDYLRYVDQPQHEWPAWTSEFVKRLACVCPLGHLHVRLVLA
                     RGNPAAEIVRLAKKQSTDLVVLVWRGKWEVPHATTLKAILHKARCPIMVLRA"
BASE COUNT          674 a         1101 c         1040 g          678 t
ORIGIN      
        1 gcgcttgggt tggcgacccc gatggcggtt atggtgggca ccggccttgg cgccatgaac
       61 ggtatcctgt tcaaaaatgc cggggccctg gaggaggcga ccaagctcga cgtcatcatt
      121 ttcgacaaaa ccggcacgct caccgtcggt cagccggaag tcgtcgatgt tgtgatatcc
      181 agacgagtgg ccacggacac tgtgctgatg accgctgctg cggtcgagca gggttccgat
      241 cacccgcttg cgcaggccat cgtgcggcgt gcgcaggggc ttgccgttcc tgtccagaag
      301 ggcttcgaga acatcgaggg catgggtgcc cgcgccgaga tcgacggtga gcccgtgttc
      361 ctgggcaatc gccggctgat ggacacgcag aagctcgatc tcggcgagct cgctgcggag
      421 gcgcaacgac tgcaaggtgc cgggcgcacc gttgttcacg tagcacgcgc cggcaaggta
      481 atcggcctta tcgccattgc cgatgcaccg cggtcgagag cggcggctac tgtcgccaag
      541 ttgcgcgaac gcggggtcca agtcgccatg ctgactggcg acaacgctgg taccgcccac
      601 agaatcgccc gcgaccttgg gcttgacatc gtgctcgccg atgtcctgcc cggtcaaaag
      661 gccggcaagg tcaaggagct gcaggcgcaa ggcaagaaag tcggcatggt cggtgacggg
      721 gtcaatgatg cccccgctct gacgcaggcc gatgtcggct tcgccatcgg cgccggcacc
      781 gacgtcgcca ttgaaagcgc tgacgtggtg ctgatgaaga gcgatccgta cgatgttgtc
      841 ggcgccgtcg agctgtccca tgccacattg cgcaagatgc atcagaacct ctggtgggcg
      901 gtgggttaca acgtcatagc ttttccgctc gcggcaggcg tcttttaccc gttcctgctc
      961 agcccggaga ttgccgctct cgccatgtcc ggcagctcgg ccctggttgc gatcaatgcg
     1021 ctgatgctca agcgcaccaa gcttgccggc atcacgcggc gcagcagcgt ccagacgtcg
     1081 ccgttgcgcg ccgcggaggt gacggcgtga gcgaagaact gggccgtcag ttgctcgccg
     1141 ggcgcgcgct gtttgcctta ctcttgggga tgtttgcgat caccgtcggc tgcggcatcg
     1201 tgctgccgat cctgcggttc ctgatcgaac gacttaccgc cacagcggat gccgcgatcc
     1261 tgtcgtggca caccggcctg ctgaccggca cttacattct ggccatcttt ctattcacgc
     1321 cgccgtggga catgagcgaa accgaacatg gtgaagaatc gtcaattcgc tcattaatgc
     1381 cgctgttccg ctactacgcg attgtccttt cgttatcact ggccgattcg cggcgcatca
     1441 acccctactc ctccgcgccg attttcttta gcagctccca tgcggcggcc gccgtaatcc
     1501 ccccgacaat gccgatgaca aagataggga tgtcagtatt gaggatccaa tgccagagca
     1561 ccccggcgat gaagctcgtt agaagcacgg tggcgacagt gatcagcaat ctctgcgatg
     1621 gtgacaccgc attctcccct tgcgcttcac aatgcgcgcc ggatggaatt tttgcgaatg
     1681 acaccgcgag gcatgagcgg ccgccgcaaa ccgagaaggg aatcagctat ccatgacctc
     1741 cacctgcagt aggctttcga cgacgacatc agcgccgtgc cgtcgcagcg catccggctg
     1801 gcggccgcga tcgatgccga tcacgcagcc gaagttcccc cgccgaccgg cttcgacgcc
     1861 ggccaaagcg tcttcgaaca ggacagcgcg agacggctca actcgtaggc ggcgggcggc
     1921 ttccaaaaaa agcgccggat ccggcttgcc cggcagcccg agcttctccg catcgatccc
     1981 gtcgacacgg acgtcgataa ggcgatcaag cctcgcagcg cgcagaatgg cggcgcagtt
     2041 ctcgctggat gagccgacgg cgatcccgat gcccacccgc cggagttctt tcagaagcgc
     2101 ttcggcgccg gacgccggct caatgccttg ttgcagggct tgccgaaaca ggcgtgtctt
     2161 gcgttcaccg atggcgcgga ccgtctctgc gtcctcagga tcatgttccg atccttccgg
     2221 cacaacgatg tttcgcgcag ccaagaagtt ccgaacgccg tcgtagcgtg gtcgaccatc
     2281 gacgtaagcg aggtagtcgg ccgcgacgtc gaacggtcgg aacgacccac catcacgttc
     2341 tgcccgcttt cgaaggaacg catcgaacac cgctttccac gcggcagcgt ggatacccgc
     2401 ggtgtcggtc agcacgccat caaggtcgaa gatcgccgcg tcgaagtcgc gccgatagat
     2461 cgtgactgag gcctcggcga ccattgcttg ctcgcggatc gggcggccaa accatcaccc
     2521 atcagtcgcc agcgcgcact gcggtgctat acggccagct ccagttctgg atttccggca
     2581 tgtcctcgcc gtgctcgtca atatagttcc ggtgtcatgt caagcaacat ttccgcgaga
     2641 agctcatcga gcacgcacgc gtacggcgcc gcgttcacga tgcccgccga atccgctcca
     2701 gcaaggtaga cgagttgctt agcgcttgga cgagcattgc tttctttcgc gttagtctca
     2761 aaagcgtgca gaaatgtcac gcccgcagca ccattatggg acaccgcgcc ttatgcaaga
     2821 tggcctttag agtcgttgca tgcggtactt cccactttcc tctccagacg aggaccacga
     2881 ggtccgtaga ttgctttttg gccaagcgca cgatttccgc cgccggattt ccacgggcga
     2941 gaactagccg cacgtgcaag tgaccgagcg gacaaacaca tgcaaggcgc ttgacaaact
     3001 cgctggtcca ggcaggccac tcatgctgcg gctgatcgac ataccgaaga tagtcggaat
     3061 ggatatcaaa cggctgaaag gctcgaccgt gctgttccgc ccaccgctgg aggaatgcat
     3121 cgaagactgt tttccatgct gcggcatgaa cccctgccat atcggtcaaa acgccgtcaa
     3181 gatcgaagat cgccgcgtca aagtcgcgcc gatttatggt cactgctgcc tcgacgagca
     3241 ttgctttctt tcgcgttggt ctcaaaggcg tgatgtcagg cccgcagcac cattatggga
     3301 caccacgcct cacgcaagat tgcctttagt gtcgccgcat ggggtacttc ccactttcct
     3361 ctccacgcga ggaccacgag gtccgtagat tgttttttgg ccaagcgcac gatttcggcc
     3421 gccggatttc cacgggcgag aactagccgc acgtgcaagt gaccgagcgg acaaatacat
     3481 gcaacgcgct tga
//
LOCUS       DATLJT010001544         5442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_375316,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001544 DATLJT010000000
VERSION     DATLJT010001544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375316"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..698)
                     /locus_tag="VM910_35305"
     CDS             complement(48..698)
                     /locus_tag="VM910_35305"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVI67813.1"
                     /translation="MPTPTCGRSPRPISIAFRRALHGAGIPGILFLMLSNGGLTHVAE
                     AKRAPVQLLESGPAAGALAGAWFSRHAGLDRVLAFDMGGTTAKLALVDEGEPLVAWDF
                     EAARQKRFLRGSGLPIQIATVELIEIGAGGGSIARASTLGTLHVGPESAGAQPGPVCY
                     GQGGSEPTITDANLILGRLGADRFLGGEMRLDVEGAKRALERQARQIGLLPPLPEL"
     gene            1132..1254
                     /locus_tag="VM910_35310"
                     /pseudo
     CDS             1132..1254
                     /locus_tag="VM910_35310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1348..2292)
                     /locus_tag="VM910_35315"
     CDS             complement(1348..2292)
                     /locus_tag="VM910_35315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67814.1"
                     /translation="MAERNLSRNTQASYRDTLTLLFPFASARAGRPIDRITVEDLTPS
                     IVRQFLDHLERDRRCSGVTRNQRLAMIHSLARFIGTHSPAHLAWCSEVRSIPFKKTAK
                     SLIGYLEKDEIDALLKQPDQRTALGARDYVLLLFLYNSGARADEAAKLAIGNLQLGRS
                     SPSARILGKGNKIRMCPLWPTTTISLSRLVIGRGADEAVFLGRTNQPITRFGVHRLVT
                     HYAKLAAKSVPSIIGKRVSPHTIRHTTAVHLLRAGVDINTIRAWLGHVSLDTTHIYAE
                     VDMEMKAKALASVDISNLHHPPQRGGPLPSLMAFLKEL"
     gene            complement(2342..3283)
                     /locus_tag="VM910_35320"
     CDS             complement(2342..3283)
                     /locus_tag="VM910_35320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67815.1"
                     /translation="MLLSEVIDAYVLLQQSLGKRFQSARTLLRRFSREMGNPDIQEVA
                     AANVAAFLRGSGQLSATWTLRYRVLSGFYRFAISRGHVDTCPLPTSQPKLPPQQSPYI
                     YSTDELRRLLNATSALDLANSRLKAMYRTLLMLLYGAGLRIGEALALTLRDVDLTERI
                     ITVRNTKFFKTRLVPIGPRLARELAAHVKRRRQLPLPAGDDSPLFTTRTCKRWSYQHV
                     IRLFQHVRRAAGIGCPTEESRPPRLHDLRHTAAVHRVVAWYRSGRDVQRLLPLLATYL
                     GHVDLRSTQRYMQMTPELLQEASHRFARYSQWGNHRE"
     gene            complement(3274..4506)
                     /locus_tag="VM910_35325"
     CDS             complement(3274..4506)
                     /locus_tag="VM910_35325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI67816.1"
                     /translation="MFEQLFSSESACRRHRDAPLADERQRYLQHCAEQGATHGTLRMK
                     AKELLWLARHLGSDASVGVDMEDLQGIVRRRQSACRGTTTVRRLIDIARPWLKFLGWW
                     RAPVVEFQFQHQLDQYAAWMRDERGLSRQTVERWHAHAKAFLQWCEKTNQRLTTLRPS
                     DIDCYFVDEGAQRRWSRVSISHTATALRAFLRYAAMLGMCDTRLAVTVRRPTVYRHES
                     LPRGPEWPDVRRILTKTDTDEPRDIRNRAILMLLAIYGMRSGEVASLRLDQIDWRARA
                     LQLFRLKRRQAQVYPLLPSVAEALARYIDTVRPPVPYPEVFIRLQAPWRPIKSSSLYD
                     VANREFVALGIQTARHGPHAFRHACAAQLLANGLTFKEIGDHLGHRSTSATATYAKVD
                     IISLREVGDFDLGELQCC"
     gene            4661..5442
                     /locus_tag="VM910_35330"
                     /pseudo
     CDS             4661..5442
                     /locus_tag="VM910_35330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         1110 a         1637 c         1712 g          983 t
ORIGIN      
        1 ttccagtcgg ggcgcatggg cgagatcacg cgcacggccg aaagccgcta aagctcgggc
       61 agcggcggca agaggccgat ctggcgcgcc tggcgctcga gcgcgcgctt ggcgccttcg
      121 acgtccaatc gcatctcgcc gccgaggaag cgatcggcgc cgagccggcc gagaatgaga
      181 ttggcgtcgg tgatggtcgg ctcgctgccg ccctgcccgt agcagaccgg gccgggctgc
      241 gcgcctgcgc tctccggccc gacatgcaga gtcccgagcg tgctcgcccg ggcaatcgag
      301 ccgccgcccg cgccgatctc gatcagctcg accgtcgcga tctggatcgg cagtccgcta
      361 ccacgcaaga agcgcttctg ccgcgccgct tcgaagtccc aggcgactag cggttcgccc
      421 tcatcgacca gggcgagctt tgccgtggtg cctcccatgt cgaatgcaag cacacggtcg
      481 aggcccgcat gacggctaaa ccaagccccc gccagcgcac ccgccgccgg accgctttcc
      541 aggagctgca ctggcgcgcg cttggcctcg gcaacatgcg tcagcccgcc attggataac
      601 attagaaaca agatgccagg aatgccggcg ccgtgcagcg cccgcctgaa ggcgatcgag
      661 ataggtctcg gcgatcggcc gcacgtaggc gttggcaacg gtcgttacca tgcggggata
      721 ttcccgcatc tcgggcgcga tatcgctgga cagagaaagc gataggtctg ggaagcgccg
      781 cgcgatggcc gctgccgcgg cgcgctcatg ggccggattc gcgtaagcat gcaggaagca
      841 gatggcgaga cttcgcacgc cgtcggcgac cagtccttcg acctcgcgca agacggccgt
      901 cacgtcgagc ggaatttcga cgctgccgtc gggcgccaaa cgttccggaa cctcgcggcg
      961 ccagggtcgc ggcacgagag gcggcggcat ttcaaggaac agatcatatg cgttagctaa
     1021 aacttaatct gacagatggt gtcctggtcc cccaggcgaa tcgcaagccg aggcggggcg
     1081 cggagctggt cggggttgcg aagcgccccg cctcggcgat tggaggacac tatgaccacc
     1141 gagcagaaaa ttatccgcgc caaggttgga ctgctggagc ttgccaaaca gctcggcaat
     1201 gtcagccagg cctgcaagat gatgggctac agccgcgaca gcttctaccg ttatgttgcg
     1261 ccgggattta tgttgcgcca cgaggagcgg gcccgggatg gcgctctttt ccttggcttg
     1321 cgcgtcacat aacgagcgac ggtcgtccta aagttctttc aggaaggcca tcaatgaggg
     1381 aagcgggcca cctcgctgcg gcgggtggtg cagattcgag atgtcgacgc ttgctaatgc
     1441 cttggccttc atctccatat cgacctccgc atagatgtgc gtggtatcca gggagacatg
     1501 gccgagccag gcccggatcg tgttgatgtc gacgccggca cgcaaaagat ggacagctgt
     1561 cgtgtggcga atcgtgtggg ggctgacgcg cttgccgata atcgagggta ccgatttggc
     1621 cgccagcttt gcatagtggg tgacgagacg gtgtaccccg aagcgggtga tcggctggtt
     1681 tgttcgaccc aagaatactg cttcatccgc accccgccca atcaccaatc gcgacagcga
     1741 aattgtcgtc gtcggccata gcggacacat ccgaattttg ttccccttgc caaggatgcg
     1801 ggccgatggg gacgaccttc ccagttggag attgcctatg gccaatttgg ctgcctcgtc
     1861 ggcccgtgca ccactgttat acaaaaagag caaaagcaca taatcgcgag cgccgagtgc
     1921 cgtgcgctgg tccggttgct tcaacaaggc atcaatctcg tctttttcca agtacccgat
     1981 caacgatttg gcggtcttct tgaacgggat cgaccttacc tcgctgcacc aggcaaggtg
     2041 agccggcgag tgcgtgccga tgaaccgcgc cagagaatgg atcatggcaa gtcgctggtt
     2101 gcgcgtgacg ccactgcagc ggcggtcgcg ctccaggtga tcaaggaact gacgaacaat
     2161 ggatggcgtc aaatcctcga ctgttatccg atcgatgggg cgtccggcgc gggcgctggc
     2221 gaaagggaac agcagggtca gcgtgtcacg atagctggct tgtgtattgc gtgatagatt
     2281 gcgctcagcc acgaggtgct ccaggaggaa gcgtcgcacc cagggaccaa caagttgcct
     2341 gtcattcacg atggttcccc cattgcgagt aacgggcaaa gcggtggctc gcctcctgga
     2401 ggagttcggg tgtcatctgc atgtagcgct gagtagacct gagatcgacg tgaccgaggt
     2461 aggttgccag tagcggcaga agccgctgga cgtcccgccc agagcgatac cacgccacga
     2521 cccggtgcac ggcagcagtg tggcgtaggt cgtgaaggcg tggcggacga ctctcctcgg
     2581 tagggcagcc tatccccgcc gcccgtcgaa catgctggaa cagccggatc acatgctggt
     2641 aagaccaccg cttgcacgtg cgcgttgtga atagcggtga atcgtcacct gcgggcaacg
     2701 ggagctggcg ccgacgtttg acatgcgcgg ccaactcacg cgcgagcctc ggcccgatcg
     2761 gcactaatcg cgtcttgaag aatttggtgt tgcggacggt gataatccgc tcggtcagat
     2821 cgacatcgcg caaggtcagg gccagtgctt ctccaatgcg caagccagct ccgtacagca
     2881 acatgagcag tgttcgatac atcgccttca gacggctgtt ggcgagatcc aaagcagagg
     2941 tggcattgag cagccgtcga agctcatcgg tggagtagat atagggcgat tgttgcggcg
     3001 gcagcttggg ttggctcgtg ggcagtggac aagtgtcgac atggcctcgg ctgattgcga
     3061 acctatagaa gccggaaagc actctgtatc ttagcgtcca agtggcgctg agctgccccg
     3121 atccccgaag gaatgcggcg acgtttgcgg ccgcgacttc ctggatatcc ggattgccca
     3181 tctcgcgact aaatcgccgc agtagcgtcc gagctgattg aaagcgcttc cccagggatt
     3241 gctgaaggag cacgtaggcg tcgattacct cgctcaacag cattggagct cccccaagtc
     3301 gaagtcgccg acttcacgga gggatatgat gtccaccttg gcataggttg cggtcgccga
     3361 cgtgctacga tgccccaaat ggtcgccgat ctccttgaag gtaagcccat tggcgagaag
     3421 ctgcgccgcg caggcatgcc ggaacgcatg agggccatgg cgggcggttt ggatgccaag
     3481 cgcgacgaac tcgcgattgg caacgtcata gaggctgctg gattttatcg ggcgccacgg
     3541 cgcctgcagt cgtatgaaaa cttcgggata aggcactggc ggccgcaccg tatcgatata
     3601 gcgggccagc gcctccgcga cggacgggag gagcgggtag acctgcgcct gtcgccgctt
     3661 caggcgaaat agttgcaggg cccgagcgcg ccaatcgatc tgatcaagcc gcagagacgc
     3721 gacttcgccg ctgcgcatac cgtagatcgc gagcagcatc agaatcgccc ggttgcggat
     3781 gtcacgaggc tcgtcagtat cggtcttggt cagaatccgt ctcacatcgg gccactctgg
     3841 tccgcgtggc aaagattcgt gccggtaaac cgtgggtcgt cggaccgtca cagccaagcg
     3901 cgtatcacac atgccgagca tcgccgcgta gcgcaggaac gcccgtagcg ccgtcgcggt
     3961 atgggagatc gaaacgcgag accaccgtcg ctgtgcccct tcgtcaacga agtagcagtc
     4021 gatgtcgctc ggccgcaggg tggtcaatcg ctgattggtc ttctcgcacc attgaaggaa
     4081 tgccttggcg tgggcgtgcc atcgttcaac ggtctgtcgg ctcagtcctc gttcgtcgcg
     4141 catccacgcg gcgtactgat cgagttgatg ctgaaactgg aactcgacga cgggagcccg
     4201 ccaccagcct agaaacttca gccacgggcg cgcgatatcg atcagccgcc gcacggtcgt
     4261 cgttccccgg caagcggact gccgccggcg cacaatgcct tggagatcct ccatgtcgac
     4321 acctacggat gcgtcggatc ccagatgtcg agcgagccac aggagctctt tggccttcat
     4381 acgtagcgtg ccgtgggtcg caccctgctc tgcacaatgc tgaaggtagc gctggcgctc
     4441 atcggcgagt ggcgcatcgc ggtgccggcg acaggcggac tcgctggaga acaattgttc
     4501 gaacatggcg aacctccaaa acggaaatca ttcgccagtc agtcccgaaa attatgttgc
     4561 ggcaaggaag cagatcattg cgactcgccc gcccggaccc cgcgcgcaaa acataaatcc
     4621 cggcgcaaca taacggtagc tatgttgtgc acaacatagc taccgcttca aagaactcta
     4681 cgacaaaggc ggcgagctgg cactgcagga gatcagccgc aagaagccgg tgctgaagaa
     4741 ccgcctaccg caggagatcg aagatgccat cgtttcactc gccattgagc aaccggcctt
     4801 cggccaggtt cgcatcgcca acgaattgag gaagcgcggg ctcgcagtct cacctgccgg
     4861 cgtgcgctgc gtctggctgc gccacgatct tgagaccatg agcaagcggc tcaaggcact
     4921 cgaggccaag agcgccgagg aaggcttggt gctgaccgag gcacaggtcg tcgcgctgga
     4981 aaaggccaag accgagaagg aggcgcacgg cgaactgcgg cgcccaggac acgttctatg
     5041 tcggcaatct caagggcgtt ggacgcatct accagcagac cttcatcgac acctacacca
     5101 aggtaagctt tgccaactct acgaccgcaa gacgcccatc acggcggccg atctgctcaa
     5161 tgaccgtgtg ctgccgttct tcgagcagca cgatgtcaag cttctgcgcg tgctgaatca
     5221 ccgcggcagc gagtcctgcg gcaaccccga acggcacgag tatgagctgt atcttgcggt
     5281 cgaggacatc gatcactcgc gcaccaagac caagagcccg cagacgaacg gcatctgtga
     5341 cgcttccaca agaccgcgct caatgagttc tatcgtgttt cgttccgcaa aaaggtatat
     5401 cgttcaatcg acgaactgca ggccgatctg gacgcgtgga tc
//
LOCUS       DATLJT010001545         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3594075,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001545 DATLJT010000000
VERSION     DATLJT010001545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3594075"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1203)
                     /locus_tag="VM910_35335"
     CDS             complement(10..1203)
                     /locus_tag="VM910_35335"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HVI67817.1"
                     /translation="MVSVKSIISQAIHREERSHEADHPSSPVAKFGADQPLPLDCGVD
                     LAPFQIAYQTYGTLNAERSNAVLVCHALTGDQHVANVHPVTGKPGWWETMVGPGRPLD
                     PDRYYIICANVIGGCMGSTGPASINPATGKLWGLDFPIITIPDMVRAQAMLLDRLGID
                     TLFCVVGGSMGGMQVLQWTAAYPERVFSALPIACSTRHSAQNIAFHELGRQAVMADPD
                     WRGGRYGDQGTHPHRGLAVARMAAHITYLSDAALHRKFGRRMQDRDLPTFSFDADFQV
                     ESYLRYQGSSFVERFDANSYLYLTRAMDYFDIAADHNGALARAFRGIKTRFCVVSFTS
                     DWLFPTSESRALVHALNASSARVSFAEIETDRGHDAFLLDVPEFFDISRAFLQSAGKA
                     RGLGD"
     regulatory      complement(1293..1372)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1587..2438
                     /locus_tag="VM910_35340"
     CDS             1587..2438
                     /locus_tag="VM910_35340"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HVI67818.1"
                     /translation="MSKPPPAPPSLAELRKEIDSIDEGMHRLLMQRGDIIDRLIQVKQ
                     TQEVGSAFRPAREADMMRRLVERHRGILPLDTVESIWRVIISTFTYVQAPFSVHADVS
                     VSEPAMRDSARFHFGFTVPYVAHFSASAAVEAVAKSKGDLALVSAISSRTPWWLTLEA
                     DGAPKIIARLPFVERADHPAALPVFVVSRVADSAMVTEVETWSVRVSGWNAEIARALS
                     PLAEIVAVPDTAFDGAALLVSISGTNRLEKIKLALIQAGASVRSAALVGSHATRYTVL
                     PNGSPKP"
BASE COUNT          471 a          818 c          815 g          433 t
ORIGIN      
        1 ccgccatctt caatcgccca atccgcgcgc cttgccggcc gattgcagga aggcccggga
       61 gatatcgaag aactcgggca catcgagcag gaacgcgtca tggccgcggt cggtctcgat
      121 ctccgcaaaa gacacccgcg cgctcgaggc gttgagcgca tgcaccagcg cacgcgattc
      181 cgaggtcgga aacagccagt cggaagtgaa cgacaccacg caaaagcgcg tcttgatccc
      241 gcggaaggcg cgcgccagcg cgccgttatg gtcggcggcg atgtcgaaat agtccatcgc
      301 gcgggtcaga tagagatagc tgttggcgtc gaaccgctcg acgaaagacg agccctgata
      361 gcgcagatag ctctcgacct ggaaatccgc atcgaaggag aaagtcggca ggtcgcgatc
      421 ctgcatgcgc cggccgaatt tccggtgcag cgccgcgtcc gacagataag tgatgtgggc
      481 ggccatccgc gcgaccgcga gtccgcgatg cggatgagtg ccctggtcgc cgtagcggcc
      541 gccgcgccag tccggatcgg ccatcacggc ttggcggccg agctcgtgga aggcgatgtt
      601 ctgcgccgag tgccgcgtcg agcaggcgat cggcagagcc gaaaagacgc gctccggata
      661 ggccgcggtc cactgcagga cctgcatgcc gcccatcgaa ccgccgacca cgcagaacag
      721 cgtgtcgatg ccgaggcgat cgagcagcat cgcctgtgcg cgcaccatgt ccggaatggt
      781 gatgatcgga aaatcgaggc cccacaattt tccggtcgcg ggattgatcg aggccggccc
      841 ggtcgagccc atgcagccgc cgatcacatt ggcgcagatg atgtagtagc gatcgggatc
      901 gagcggccgg ccgggtccga ccatggtttc ccaccagccg ggcttgccgg tgaccggatg
      961 cacattggcg acatgctgat cgccggtcag cgcatggcag accagaacgg cgttggagcg
     1021 ctcggcgttg agcgtgccat aggtctgata ggcgatctgg aacggcgcca ggtcgacacc
     1081 gcaatcgagc ggcagcggct gatccgcgcc gaatttcgcg acaggcgaac tcgggtgatc
     1141 cgcctcgtgc gagcgctcct cgcgatggat cgcctgactt atgatcgact ttacgctcac
     1201 catcttttcg tccccgcgca acccgcgagg gctcctaaag caagactatc aggccattaa
     1261 aaaacccggc ctgaacaggg gttcggccgg gatttcatct gtccccggcc tgtttagcga
     1321 gttgtttaac gtggctgcaa gccggccggc tcaaatgacc acggaacgac acaaaattag
     1381 tcgctggccc ggtgctagtc aaggcaaggc gatggttaac agggggcagg aaccctcttg
     1441 gcgagcaggc gcgcagacaa gtttaggcag tccgcgtaga cttgactgct ccgccgtctc
     1501 aaaccatgag ggggaacgga cctccatcct tcgcgacgcg cttcacgctg cgcgggatga
     1561 agcgtgagat ttcactgggt tctcagatgt caaagccacc ccccgccccg ccgtcgcttg
     1621 ccgagttgcg caaggaaatc gacagcatcg atgaaggcat gcaccgcctg ttgatgcagc
     1681 gcggcgatat catcgatcgc ctgatccagg tgaagcagac ccaggaagtg ggctcggcgt
     1741 ttcgccctgc ccgcgaggcc gacatgatgc ggcgcctggt cgagcgtcat cgcggcatcc
     1801 tgcccctcga cacggtcgag agcatctggc gcgtcatcat ctcgaccttc acctatgtgc
     1861 aggcgccgtt ttccgtgcat gccgacgtct ccgtgagcga gcccgcgatg cgcgactcgg
     1921 cgcgctttca tttcggcttc accgttccct atgtcgcgca tttttccgct tcggccgcgg
     1981 tcgaggcggt cgcgaaatca aagggcgatc tcgccctggt ctcggcgatc tcaagccgca
     2041 cgccctggtg gctgacgctg gaagccgatg gcgcgccgaa gatcatcgcg cggctgccgt
     2101 tcgtcgagcg tgccgaccat ccggcggcgc tgccggtgtt cgtggtctcg cgcgtcgccg
     2161 acagcgcgat ggtgacggag gtcgagacct ggagcgtgcg cgtatccggc tggaatgccg
     2221 agatcgcgcg ggcactgtcg ccgctcgccg agatcgtcgc ggtgcccgac accgccttcg
     2281 acggcgcggc actcctggtg tcgatttccg gcacgaaccg gctggagaag atcaagctgg
     2341 cgctgatcca ggcgggcgct tcggtgcgct ccgcagccct cgtcggcagc cacgccacac
     2401 gctatacggt gcttcccaac gggtctccga agccgtaaaa gccgccccgt tacttccgga
     2461 gttgacgatg aaccgccccg tgccgaaccc cggcattctc gatattgcgc cctacacgcc
     2521 gggaaaaagc ccggtgc
//
LOCUS       DATLJT010001546         3107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_607473,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001546 DATLJT010000000
VERSION     DATLJT010001546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607473"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VM910_35345"
     CDS             complement(<1..1094)
                     /locus_tag="VM910_35345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVI67819.1"
                     /translation="MNEQSQSLRNFTINFGPQHPAAHGVLRLVLELNGEVVERVDPHI
                     GLLHRGTEKLIEQKTYLQAIPYFDRLDYVAPMNQEHAFCLAAEKLLGIEVPRRGQLIR
                     VLYCEIGRLLSHLLNITTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHAAFFRV
                     GGVHQDLPSKLIDDIEAFCDPFLKVVDDLDRLLTGNRIFKQRNVDIGVVTLKQAWEWG
                     FSGVMVRGSGAAWDLRKAQPYECYAEMDFDVPIGKNGDCYDRYCIRMEEMRQSVRIMR
                     QCIQKLRAPEGQGPVAIQDNKIFPPRRSEMKRSMEALIHHFKLYTEGVHVPAGEVYAA
                     VEAPKGEFGVYLVADGSNKPYKCKIRAPGFA"
     gene            complement(1100..1714)
                     /locus_tag="VM910_35350"
     CDS             complement(1100..1714)
                     /locus_tag="VM910_35350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVI67820.1"
                     /translation="MATSEILASLGEKIVSALPGAALGHAVAFDQLTVTVQAEKIVEV
                     AKYLRDTPDCRFVNITDITAVDYPGREKRFDVVYHFLSPTLNARIRVRAEADETTLVP
                     SIIDVFPGADWFERETYDLYGVIFTGHPDMRRILTDYGFEGHPLRKDFPLTGFVEVRY
                     DDQEKRVLYEPVRLNQEFRKFDFLSPWEGADYPLPGDEKAGPKA"
     gene            complement(1718..2047)
                     /locus_tag="VM910_35355"
     CDS             complement(1718..2047)
                     /locus_tag="VM910_35355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HVI67821.1"
                     /translation="MKMTDIPFGTTDWSSIEPTMHRGDSGVATWRTRQFGDIRVRLVE
                     YSPGYVADHWCQKGHILFCVDGELGTELADGRWFTLRPGMSYQVADGAEAHRSRTEKG
                     ARLFIVD"
     gene            complement(2052..2648)
                     /locus_tag="VM910_35360"
     CDS             complement(2052..2648)
                     /locus_tag="VM910_35360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HVI67822.1"
                     /translation="MGLNTNAATTGTGPVIAPAATGILDPSTGRPVGANDPYFLEVNH
                     ELSDKGFFVAAADDLIIWARTGSLMWMTFGLACCAVEMMQVSMPRYDVERFGFAPRAS
                     PRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCD
                     RIVPIDIYVPGCPPTAEALLYGVLLLQKKIRRTGTIER"
     gene            complement(2639..3004)
                     /locus_tag="VM910_35365"
     CDS             complement(2639..3004)
                     /locus_tag="VM910_35365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVI67823.1"
                     /translation="MAGILQNYLPLVVFIGVAAVIGLVLLIAPFVVAFQQPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVAYGKLGATGFWSMVVFL
                     AVLTVGFAYEWKKGALEWD"
BASE COUNT          622 a          978 c          930 g          577 t
ORIGIN      
        1 gcaaaaccgg gcgcgcgtat cttgcacttg tagggcttgt tgctgccgtc cgcgacgaga
       61 tagacgccga actcgccctt gggtgcttcc accgcggcat agacctcgcc ggccggcacg
      121 tggacgcctt cggtgtagag tttgaagtgg tggatcagcg cttccatcga gcgcttcatc
      181 tcgctgcgtc gtggcgggaa gatcttgttg tcctggatcg ccaccggccc ctggccctcc
      241 ggcgcccgca gcttctggat gcactgcctc atgatgcgca cggactgacg catctcttcc
      301 atgcggatgc agtaccggtc gtagcagtcg ccgttcttgc cgatcggaac atcgaagtcc
      361 atttcggcat agcattcgta gggctgcgcc ttgcgcaggt cccacgccgc gccggagccg
      421 cgcaccatca cgccggaaaa accccactcc caggcctgct tcagcgtaac tacgccgatg
      481 tcgacattgc gctgcttgaa gatacggttg cccgtaagca ggcgatcgag gtcatcgacc
      541 actttcagga aggggtcgca gaacgcctcg atgtcgtcga tcagctttga cggcaggtcc
      601 tggtgcacgc cgccgacacg aaagaatgcc gcgtgcatgc gcgagcccga cgcgcgctcg
      661 taaaacacca tcagcttttc gcgctcttcg aaaccccaca gcggcggcgt gagcgcaccg
      721 acgtccatcg cctgcgtggt gatgttgaga agatgcgaca agagacggcc gatctcgcag
      781 tagagaacgc ggatcaactg gccgcgacgc ggtacctcga tgccgagcag cttttcggcc
      841 gcaagacaaa acgcgtgctc ctggttcatc ggggcgacat agtcgagccg gtcgaaatac
      901 ggaatcgcct gcagataggt cttctgctcg atcagcttct cggtaccgcg atgcaagaga
      961 ccgatatgtg gatcgacgcg ctcgaccacc tctccgttca gttccaggac aaggcgcaaa
     1021 actccgtgag cggcgggatg ctgcggtccg aagttgatcg taaagttgcg cagactttgg
     1081 ctttgctcat tcatggcgat caggcctttg gtcctgcctt ctcatcaccc ggtagcggat
     1141 agtccgcgcc ttcccagggc gagaggaaat cgaacttgcg gaattcctgg ttaaggcgga
     1201 cgggctcgta aagcacccgc ttctcctggt cgtcgtaacg cacctcgaca aaaccggtga
     1261 gcgggaaatc cttgcgcaac gggtggccct cgaaaccgta gtccgtcagg atgcgccgca
     1321 tgtcgggatg gccggtgaag atgacgccgt agagatcgta ggtttcacgc tcgaaccagt
     1381 cggcgccggg aaagacatcg atgatcgacg gcaccagcgt cgtctcgtcg gcctcggccc
     1441 gcacgcggat gcgcgcgttc agcgtcggcg acaggaagtg atagaccacg tcgaaccgct
     1501 tctcgcgccc gggatagtcg actgcggtga tgtcggtgat attgacgaaa cggcaatcgg
     1561 gcgtgtcgcg gaggtatttt gcgacctcga cgattttttc cgcctgcacc gtcacagtga
     1621 gttggtcgaa cgccaccgca tgtccgagag ccgcgcccgg aagcgcgcta acgatcttct
     1681 ctccaaggct ggccagtatt tcgctcgtcg ccatgcatca atccacaata aacaggcgcg
     1741 cgcccttttc ggtgcgcgag cgatgcgctt cggccccatc ggcgacctgg tagctcatgc
     1801 ccggcctcag cgtgaaccaa cgaccgtcgg caagctccgt ccccagttcg ccatcgacgc
     1861 aaaacaggat atgccccttc tgacaccaat gatccgcgac atagcccggc gaatactcga
     1921 cgagccgcac gcgaatgtcg ccgaactgcc gggtccgcca ggtcgcaaca ccgctgtcgc
     1981 ccctgtgcat ggtgggctca atgcttgacc agtcggtggt cccgaacggg atgtcggtca
     2041 tcttcatcgc gttatcgttc gatggtgccg gtgcggcgga tcttcttctg cagcaacagt
     2101 acgccgtaga gcagcgcctc cgccgtcggc gggcaaccgg gcacgtagat gtcgattggg
     2161 acgatgcggt cgcaaccgcg caccaccgag taggaatagt ggtagtagcc gccgccattc
     2221 gcgcacgatc ccatcgaaat cacatagcgc ggctccggca tctgatcgta gaccttgcgc
     2281 agcgccggcg ccatcttgtt ggttagcgtc cccgcgacga tcatgacgtc ggactggcgc
     2341 ggcgaggcac gcggcgcaaa cccaaagcgc tcgacgtcgt agcgcggcat cgagacctgc
     2401 atcatctcca ccgcgcaaca ggccaacccg aaggtcatcc acatcaggga gccggtgcga
     2461 gcccagatga tgaggtcgtc cgcggccgcg acaaagaatc ccttgtccga gagttcgtga
     2521 ttgacttcga ggaaatacgg atcgttcgcg ccgaccggcc tgccggtcga aggatcaagg
     2581 attccggtcg ccgccggggc gatcaccgga cccgttccag tcgttgcggc gttggtgttc
     2641 aatcccattc cagcgcgcct ttcttccatt catacgcaaa gccgacggtc agcaccgcga
     2701 ggaaaaccac catcgaccag aagccggttg cgccgagctt tccgtaggcg accgcccaag
     2761 ggaacaggaa ggccacctcg aggtcgaaaa tgatgaaaag aatcgcgacc agatagaagc
     2821 ggacgtcgaa cttcatgcgg gcgtcgtcga aggcgttaaa tccgcactca tacgccgaca
     2881 gcttctccgg gtcgggttgc tggaacgcca ccacgaaggg cgcgatcaac agcaccagcc
     2941 cgatcacggc cgccacgcct ataaacacca caaggggaag atagttctgc aggatgccgg
     3001 ccatcggcgc ttaccttttc ccgcggtcgt cggagcccgc ggattcgtcg ctttggaatt
     3061 attctgaggc ttagcgcagc gcaacagagg gcgcaagaca agctatt
//
LOCUS       DATLJT010001547         1839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1756346,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001547 DATLJT010000000
VERSION     DATLJT010001547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756346"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /gene="purH"
                     /locus_tag="VM910_35370"
     CDS             complement(<1..897)
                     /gene="purH"
                     /locus_tag="VM910_35370"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HVI67824.1"
                     /translation="MSDHPRRVTRALLSVSDKAGLIDFARALAGHGVELVSTGGTAKA
                     IAQAGLQVRDVSDLTGFPEMMDGRVKTLHPKVHGGLLAIRDNKDHAQAMKMHGIAPID
                     LLVVNLYPFEATVDKGAGFEECIENIDIGGPAMIRAAAKNHDDVAVVVEAGDYQAVLD
                     ELKMYQGSTTLALRRRLAAKAYARTAAYDAAISNWFAAELKIEAPDFRSFGGRLIQTL
                     RYGENPHQSAAFYRLPDRRPGVSTARQLQGKELSYNNINDTDAAYEAVSEFDPKRTAA
                     CVIVKHANPCGVAEGKDLVSAYR"
     gene            1020..1457
                     /locus_tag="VM910_35375"
     CDS             1020..1457
                     /locus_tag="VM910_35375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HVI67825.1"
                     /translation="MHEPAMEFEGKVLSPQKEVVRIFFKELWDHADKSLIPKLFHADF
                     TFRGSLGRELRGHEQFAGYVDLVTTALGDYTSDILDMVEEGNKVWAKLRYHGYHRGEL
                     LGYPPTGKHVWWYGTPFFTFEGAKVRDLWVLGDIHGLIGRLSA"
     gene            complement(1503..>1839)
                     /locus_tag="VM910_35380"
     CDS             complement(1503..>1839)
                     /locus_tag="VM910_35380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HVI67826.1"
                     /translation="APGARVRESDFALRFHLHPSVKASRLSDARGVMLVLPNREVWTF
                     EALDDKVELEDSVFLAGSDGPRRTAQIVIRQDSRHASAVRWSFVRSQSSPAATSARRN
                     ARREPELPL"
BASE COUNT          317 a          573 c          598 g          351 t
ORIGIN      
        1 gcgataggcg ctgacgagat cttttccctc ggcgacgcca cagggattgg catgcttgac
       61 gatgacgcag gccgcggtgc gcttcggatc gaactcgctg acggcctcgt aagccgcgtc
      121 ggtgtcgttg atgttgttgt aggacaattc cttgccctgc aactgccgcg cggtggaaac
      181 gccggggcgc ctgtcgggca gccggtagaa ggcggcgctc tggtgcgggt tctcgccgta
      241 gcgcagggtc tggatcagcc tgccgccaaa tgagcggaaa tccggcgcct cgattttgag
      301 ttctgccgcg aaccagttcg agatcgcggc atcgtaggcg gccgtgcgcg cataggcttt
      361 ggcggcgaga cggcggcgca acgcaagcgt ggttgaaccc tgatacatct tgagttcatc
      421 aagcaccgcc tgatagtcgc cggcttcgac cacgaccgcc acgtcgtcgt ggtttttggc
      481 ggctgcgcgg atcatcgccg ggccgccgat atcgatgttc tcgatgcact cctcgaagcc
      541 tgcgcccttg tcgacggtcg cctcgaacgg atagaggttg acgacaagaa gatcgatcgg
      601 cgcgatcccg tgcatcttca tcgcctgcgc gtggtccttg ttgtcgcgga tcgcaagcag
      661 gccgccatgc accttcgggt gcagcgtctt gacccgtccg tccatcatct cgggaaagcc
      721 ggtcagatcg gagacgtccc tgacctgaag cccggcttgc gcgatcgctt tggcggtgcc
      781 gccggtcgaa acgagttcga caccgtggcc ggcgagagcg cgggcgaagt cgatcagacc
      841 ggccttgtcg gaaaccgata acagggcgcg ggtgacgcgg cggggatggt cgctcatgtc
      901 gagatcctgg ctgtcaaggc cgccgggtag catggttttg ccggcttcga aaccggccgc
      961 atgtggaaat ctccgaccgt ttcgggcatt tttcgtcgtg cacggtctgg agcatcgcca
     1021 tgcatgagcc agcgatggag ttcgagggca aggtcctgtc accccagaag gaagttgtcc
     1081 gcatcttctt caaggagctg tgggaccacg ccgacaagag cctgatcccg aagctgttcc
     1141 atgcggactt cacttttcgc ggctcccttg gtcgggagct tcgcggccac gagcaattcg
     1201 caggttatgt cgacctcgtc accacggcgc ttggcgacta cacctccgac attctcgata
     1261 tggtggagga aggcaacaag gtctgggcca agctgagata tcacggctat caccgcggtg
     1321 aactgctcgg ctatccaccg accggcaagc atgtctggtg gtacggcacg ccgttcttta
     1381 ccttcgaagg cgcaaaggtc cgagacctct gggtcctcgg cgacatccac gggctgatcg
     1441 gaagactgtc agcctgaagc gcgatgccgt tgggtcgaat cccgatcgcg ccggccaact
     1501 cgctacagcg gcaattccgg ctcacgccgt gcgttccgcc gggcgctggt cgcggccggc
     1561 gagctctgcg agcgcacaaa gctccagcgc accgcggagg cgtggcgcga atcctgccgg
     1621 atcacgatct gggcggtgcg ccgtggtccg tcactgccgg ccagaaatac actgtcttcg
     1681 agctcgacct tgtcgtccag cgcctcgaac gtccagactt cccggttcgg cagcaccagc
     1741 atgaccccgc gtgcatcgga aagccggctt gccttcaccg acgggtgcag gtgaaaccga
     1801 agcgcgaagt cggactcgcg gacgcgcgcg cccggggcc
//
LOCUS       DATLJT010001548         3929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4281921,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001548 DATLJT010000000
VERSION     DATLJT010001548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281921"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VM910_35385"
     CDS             complement(<1..1116)
                     /locus_tag="VM910_35385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67827.1"
                     /translation="MSALDASGEADLLASAKGARSAQAAQIANIAARLERLPLTSYQK
                     AIFAIIATAWFFDSMDLGALTFVLGSIKQTFQLSTAEAGLLSSLSFLGMFVGAASAGL
                     LADRFGRAKVFQASMIFWGLGSLACGLSSTVAMLGASRLLLGFGMGMEFPVAQSMVSE
                     IIPAERRGRYIAYLEGFWPLGFIASGLLIYVVLSVADWHWVFILQAIPALFVLVIRRY
                     VPESPRWLAAHGHSERAETVMQGIESKVAARLGGKALPSPRPQAIAAPARVNIATLFS
                     GIYARRTTMLWMLWFFALLGFYGLTTWLGALLQAKGFPVTKSVFYTILISLAGIPGFL
                     FSAWLVESWGRKGTLTLNLIGGAVACYFYGGAADQTQL"
     gene            1385..1876
                     /locus_tag="VM910_35390"
     CDS             1385..1876
                     /locus_tag="VM910_35390"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HVI67828.1"
                     /translation="MNGFLRSLDRLIGALVAAAKWLALPLVVLLFLQWPLRDLVRSFS
                     REANDLGQIAFALFVAVSVTAATRAGTHLAVDLVARHYSTRTRRMLARAGSALGLLPW
                     ALFVLIAGKNAVISSVRDLEAFQDSGNPGYFMIKIALWVMAAAVIGQCLVDTFRPLEA
                     DDK"
     gene            1876..3330
                     /locus_tag="VM910_35395"
     CDS             1876..3330
                     /locus_tag="VM910_35395"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVI67829.1"
                     /translation="MAWLGLALLVLVGFGIIFTGLPAAVILVASATLGAILGIVSGTI
                     EISTLGALPSRLVNLFENDLLQALPLYVTMGLLLDRLPVADALYRASNAALPRKPSAP
                     LVSGMVLGALLGPMNGSVGASVLGLSRVVAPRLAAEGIPAPSRQAMVAVASTLGVLVP
                     PSLVLILLSDAMLSAHTMAVTATGRNDRVVNTQDIFHAALLPGAIFLAMCLALSWFVG
                     RAATKLPEREPLTKGEGWLAAIALVSLVGLLGGVSLGYFYAVEAAAMGAFALLVAGLV
                     TGRLHGEVLNKLLNDAIALTGALFSLLLAATTFTMVLRLLGTADIVGRMVSSIPGGEI
                     AAVASVLAVIGLCAFVLDAFEIIFVIVPIVIPPLLIRVADARWVSVLVLLTLQSSFLL
                     PPFGYALMMVRGVLREPVAFRPFLRALAPYLAAQWLLLLVVLFFPQLTHMGHSAADAS
                     RAPAAPMSDAEFNKRLQEMIGPPEPGELGDPNRR"
     gene            complement(3351..>3929)
                     /locus_tag="VM910_35400"
     CDS             complement(3351..>3929)
                     /locus_tag="VM910_35400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVI67830.1"
                     /translation="KSIAEIESLAAATQRIDGVLQLIQAIAEQTNLLALNATIEAARA
                     GDAGRGFAVVAHEVKALAGQTAKATDEIGQNVSLIQSSTRNAVDAVREIGEAVREINK
                     ITSAIAGAIGQQDSATREISTNAQSAAEGNETLVSNIASLRDAIGETDTAAASVLTAS
                     GELTATAETLSREVEKFFQNLRASPAESRKAA"
BASE COUNT          613 a         1251 c         1301 g          764 t
ORIGIN      
        1 gagctgggtc tggtccgcgg cgccgccata gaagtagcag gcgacagcgc cgccgatcag
       61 gttcagcgtg agcgtgccct tgcggcccca ggattccacg agccaggccg agaacaggaa
      121 acccgggatg cccgcaagcg aaatcaggat ggtgtagaac accgatttgg tgaccggaaa
      181 accctttgct tggagcagcg cgccgagcca ggtcgtcaag ccatagaagc cgagcagcgc
      241 gaagaaccac agcatccaga gcatcgtggt gcgtctggca tagatgccgg aaaacagcgt
      301 ggcgatgttc acgcgcgccg gcgctgcaat agcctggggt ctcggtgaag gaagcgcctt
      361 gccgccgagc cgcgcagcga ccttggactc gatcccctgc atgaccgttt cagcccgctc
      421 ggagtggccg tgggcggcga gccagcgcgg cgattccgga acgtaacggc ggatcaccag
      481 cacgaacagc gccgggatcg cctgcaggat gaatacccag tgccagtctg cgaccgacaa
      541 cacaacatag atcagaaggc ccgaggcaat gaagccgagc ggccagaagc cttcgagata
      601 ggcgatgtag cggccgcgcc gttcggccgg gatgatctcc gacaccatgg attgcgcgac
      661 gggaaattcc atgcccatgc cgaagccgag caacagacgc gaggcgccga gcatggcgac
      721 tgtcgatgac aggccgcagg cgaggctgcc gaggccccag aagatcatgc tggcctgaaa
      781 aaccttggct cggccgaagc ggtccgccag gaggcctgca gaggctgcgc ccacgaacat
      841 gccgaggaag ctcaagctgg acaggaggcc cgcttctgcg gtcgaaagct ggaacgtctg
      901 cttgatcgag ccgagcacga aggtcaacgc gccaaggtcc atgctgtcga agaaccaggc
      961 ggtggcgatg atcgcgaaga tggccttttg ataggacgtc agcggcagcc gttcgaggcg
     1021 ggccgcgatg ttggcgatct gggcggcctg tgcgctgcgc gcgccctttg cggaggccaa
     1081 tagatcggct tcgccggaag cgtccagagc actcatgttt cgtctccccg aatgaccccg
     1141 ggctctttct ggctccggat tcagggaatc tcatatgagc acgactgata tgtcaataat
     1201 atatttgagc gcgacgcacc ggttgcgcgg atggcgctcc gatcggacgg atgccgctgg
     1261 tcgcgcggcc tggatgcgcg ctttatgatg cggcgcgggc ggctttgcgc atcgccgcta
     1321 gccagcgcgc ccgcgatctg gaacattgcg ttgcaagggc gagcctttgg ggagggtggc
     1381 cactttgaac gggttcctgc gcagtctcga tcgcctgatc ggcgcgcttg tcgcggccgc
     1441 caaatggctt gcgctgccgc tggtcgtgct gttgtttctg caatggccgc tgcgcgatct
     1501 cgtgcggagc ttttcgcgcg aagccaacga tctgggccag atcgcgtttg cgctcttcgt
     1561 cgcggtcagc gtcaccgcgg cgacgcgcgc gggcacacat ctcgcggtcg atctcgtcgc
     1621 caggcactac tcgacgcgaa cgcgaagaat gcttgcgcgc gccggcagcg cgctcggctt
     1681 gctgccgtgg gcgctgttcg tgctgattgc cggcaagaac gcggtgattt cgagcgttcg
     1741 cgatctcgaa gcgtttcagg actcgggcaa tcccggctac ttcatgatca agatcgcgct
     1801 ctgggtgatg gcggccgccg tcatcggcca gtgcctcgtc gacacgttcc gtccactcga
     1861 agcggacgac aagtgatggc gtggctggga ctcgcgctgc tggtgctggt cggcttcggc
     1921 atcattttca ccggtctgcc cgccgctgtc atcctggtgg cgagcgccac cctcggcgcc
     1981 attcttggca tcgtgagcgg caccatcgag atttcgacgc tcggcgccct gccaagccgg
     2041 cttgtcaatc tcttcgagaa cgacctgttg caggcgctgc cgctctacgt gacaatgggc
     2101 ctgttgcttg accgccttcc ggttgcggat gcgctctatc gtgccagcaa tgccgcgctt
     2161 ccgcgaaaac cgtcggctcc gctggtgtcc ggcatggtgc tcggcgcctt gctcggtccg
     2221 atgaacggct cggtcggcgc cagcgtgctg ggcctgtccc gcgtggtcgc gccgcgtctt
     2281 gcggccgaag gcatccccgc gccgagccgc caggcgatgg tggcggttgc gagcacgctc
     2341 ggcgtattgg tgccgccgtc attggtgctc attctcctga gcgacgccat gctgtcggcc
     2401 cacaccatgg cggtcaccgc gacgggacgg aacgaccgcg tcgtcaatac acaggacatc
     2461 tttcatgcgg cgcttctgcc gggtgcgatc tttttggcaa tgtgccttgc gctatcctgg
     2521 ttcgtcgggc gtgcggcaac aaaactgccg gaacgcgagc cgttgaccaa aggcgaggga
     2581 tggctcgccg caatcgcgct cgtctcgctg gtcggactgc tcggcggcgt cagcctcggt
     2641 tatttctacg ccgtggaagc cgccgccatg ggcgcgtttg cgctgctcgt cgccggcctc
     2701 gtgaccgggc gcttgcacgg cgaggtgctc aacaagctgt tgaacgacgc gatcgcgctg
     2761 accggcgcct tgttctccct gctgctcgcg gccacgacgt tcacgatggt gttgcggctt
     2821 ttgggcaccg ccgacatcgt cggcagaatg gtgtcatcga tcccgggcgg cgagatcgcc
     2881 gcggtcgcca gcgtccttgc cgtgatcggg ctttgcgcct tcgtgctcga cgctttcgag
     2941 atcattttcg tgatcgtgcc gattgtgatc ccgccgctcc tgatccgtgt tgcggatgcg
     3001 cgctgggttt cggtgctggt gctcctgacc ctgcaatcga gcttcctgct gccgcctttc
     3061 ggctatgcgc tgatgatggt gcgtggcgta ttgagggaac ccgttgcctt ccgtccgttc
     3121 ctgcgtgcgc tcgcgcctta tcttgcggcg cagtggctcc tgctgctggt cgtcctgttc
     3181 tttccgcaac tgacccacat ggggcattcc gccgccgacg cctcgcgcgc gccggctgcg
     3241 ccgatgtcgg acgccgagtt caacaagcgg ctccaggaga tgatcgggcc gcccgagccg
     3301 ggcgaactcg gcgatccaaa tcggcggtga tctgaaggca agcgaaagtg tcacgccgcc
     3361 ttgcggcttt cggccgggct ggcgcgcagg ttctggaaga acttttccac ctcgcgtgac
     3421 agcgtctctg ccgtggccgt caactcgccc gaggccgtca gcaccgaggc tgcggccgtg
     3481 tcggtttcgc cgatcgcgtc gcgcagcgaa gcgatgttcg acaccagcgt ctcgttgcct
     3541 tctgccgccg attgcgcgtt ggtggagatt tcgcgggtcg cggagtcctg ctggccgatc
     3601 gcgccggcga tggccgaggt gatcttgttg atctcgcgca ccgcctcgcc gatctcgcgc
     3661 acggcatcga ccgcattcct ggtcgaagat tggatcaagc tgacgttctg cccgatttcg
     3721 tcggtcgcct tcgcggtctg ccccgccagc gccttcactt catgggcgac cacggcgaag
     3781 ccacggcccg catcgccggc gcgcgcggct tcgatggtgg cgttcagcgc caaaagattg
     3841 gtctgctcgg cgatcgcctg gatcaattgc agcacgccgt cgatccgctg cgtcgcggca
     3901 gcgaggctct cgatttcggc gatcgactt
//
LOCUS       DATLJT010001549         4280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4053974,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001549 DATLJT010000000
VERSION     DATLJT010001549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4053974"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VM910_35405"
     CDS             <1..216
                     /locus_tag="VM910_35405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67831.1"
                     /translation="YEGRDRKTGEVKWTGTRADLIFGSHSQLRALAEVYACADSKDKF
                     VKDFVAAWTKVMNLDRFDLDSSRRKAL"
     gene            complement(312..1172)
                     /locus_tag="VM910_35410"
     CDS             complement(312..1172)
                     /locus_tag="VM910_35410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67832.1"
                     /translation="MKKLSSVLLLASLNLLAETVLMPVSAQQLAPPNETSQAGALLTP
                     RIERVPTPATRLHMGMTEADVEQTMGAAAEVATSENAGVKIRVLKYRLSPIPAKITIS
                     DGRLSGVVLDIAAADDRELPAHSRSVWPGMHRRTVLRMLGAPAEDRFHKSFGLKLEHM
                     IFARAGQTDLTVILIDDHVVTKKVGRALPPDIFAFSLPLATGEADQPVERPRSTRMHV
                     GMSMGDVQAAFGAPKLSVPFTFKGHPAEYRIHETDQNGSFACFDFIDEVLVGFSDGGK
                     IPLDQILSGG"
     gene            complement(1573..1920)
                     /locus_tag="VM910_35415"
     CDS             complement(1573..1920)
                     /locus_tag="VM910_35415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67833.1"
                     /translation="MALFKLPLSGDANQWINPITSWWSGNQISVNLGESGSPETEAEI
                     LRRVGTYGRQLGQITDAVVVLLQHLPKHTSLSAEESEAIKAFEEMANDIAKIKESHKL
                     TALRPRKSVAASS"
     gene            complement(2076..3242)
                     /locus_tag="VM910_35420"
     CDS             complement(2076..3242)
                     /locus_tag="VM910_35420"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI67834.1"
                     /translation="MNIQTRPDPVARARELGTEIAAAADEIERTRRIPEALLERFHDS
                     RLFRMLLPRSAGGDETEPAVYVAAIEELARHDASIAWNAFVANSSSLIAAYLDPGVNR
                     AIFADPRSIVAWGPPNASRARAVDAGYRLTGKWDFASGCRHARWLGAHCHVLEADGTL
                     RLNRFGRPAVRTLLFPAGEATLLDTWRTIGLCGTASDSYCVNDLFVSEAFSSTREDPT
                     LRREHGPLYAFTMQCLYATGVAAVAFGIARAMLSEFITLASRKAPRGLARLADNAVVQ
                     ADVARAEARLGSARAYLIETLTAIYAHADDVAPIEVADRARVRLACTNAIQGAAEVAD
                     FAYKGAGVDAIFTGSPFERRFRDMHTLSQQIQARGAHFESVGQVMLGVPPEVFL"
     gene            4021..>4280
                     /locus_tag="VM910_35425"
     CDS             4021..>4280
                     /locus_tag="VM910_35425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67835.1"
                     /translation="MAGVGIAMNGWFARSLGASDAAGWLFFATGVASDLVALVVPSCS
                     ARLWQARHRATSLAGWAVWAMTFVFAVTAGVGFASTNIADVTL"
BASE COUNT          816 a         1297 c         1341 g          826 t
ORIGIN      
        1 tatgaggggc gcgaccggaa gacgggtgag gtgaagtgga ccggcacccg cgcggacctg
       61 atcttcggct cgcactcgca gttgcgggca ctcgcggaag tctatgcctg cgcggactcg
      121 aaggataagt ttgtgaaaga cttcgtggcg gcgtggacca aggtgatgaa tctggaccgc
      181 ttcgacctcg actcatctcg tcggaaagcc ctttaagggc ttccggcggg ctgtcgaaga
      241 agatggtttg gcaagcgaaa gcgccggcca aagtgccggc gtttcgaaat tagttcataa
      301 gcttcgctcc ctcagccgcc gctcaggatc tgatcaagcg gtatcttgcc gccgtcggaa
      361 aagccgacca gcacctcgtc gataaagtca aagcatgcga acgatccgtt ctggtccgtt
      421 tcatgaatcc tgtattcggc gggatgtccc ttgaaggtaa agggcactga cagcttgggt
      481 gcgccgaacg ctgcctgcac atcacccatg ctcatgccga cgtgcattcg cgtgcttcga
      541 ggccgctcga ctggctgatc agcctcgcct gttgccagag gtaacgagaa agcgaagata
      601 tccggcggca gtgcgcgccc gaccttcttc gtcaccacgt gatcgtcgat caggatgacg
      661 gtgagatcag tctgtcccgc gcgcgcgaag atcatgtgct cgagcttcaa accgaagctc
      721 ttgtggaagc ggtcctcggc gggcgctccc aacatccgca gcacggtcct ccggtgcatt
      781 ccgggccaca ccgatcgact gtgagcgggc aactcgcggt catcagcggc ggcgatgtcg
      841 agcacgacgc ccgaaaggcg gccgtcggaa atcgtgattt tggcagggat cggcgagagc
      901 cggtatttga gcacgcggat cttgacgccg gcattttcgg acgtggcgac ttctgccgct
      961 gctcccatcg tctgctcgac gtcggcctcc gtcatgccca tatgcagtcg cgttgctggt
     1021 gtcggaacac gttcaatacg tggcgtgagc agtgcaccgg cttgcgaggt ttcgttcggg
     1081 ggagcgagct gctgcgccga tacaggcata aggacagttt cagccagcag gttcaggctc
     1141 gctagcagca ataccgaaga tagctttttc acggaatcct cctgtcgagc gttaatgcag
     1201 cttggaggtc ttcacctgct tcaatggcga acggcaggtt tgtgcgatgt agagcgccaa
     1261 cggctcttcg tcctgtaaaa tgttgcgcgc caaaacgcag ggcaatatta cggccgagac
     1321 cacaacgggc ctgtgaaatc gttcacacgc acgctgcgca ggatgcaaac gggacagata
     1381 gtacgtccgg tgactgccca catggaaggc gcaacatgaa tggcgaggcg cggctcgcgt
     1441 gtaaggctgg cgtaagccga ctggggacga gcccggaaat tatgccgaaa gtcgtccacc
     1501 ctcggaccgc aaaggccgac agcccctgga agcctctgga accggcgccc aacggcgatc
     1561 gtaacgggag cttcaggacg aagctgctac gctctttcgc ggacgcaacg ccgtgagctt
     1621 atgactctct ttaattttgg cgatatcgtt agccatctcc tcaaatgctt tgatcgcttc
     1681 gctctcttct gcggaaagag acgtgtgttt tggcaaatgc tgcaatagca cgaccacagc
     1741 gtcggtgatc tgtccaagct gccgtccata ggtgccgact ctgcggagga tttcggcctc
     1801 ggtctcgggc gagccggatt caccgagatt tactgaaatc tggttgccgc tccaccatga
     1861 agtgatcggg ttgatccact gattggcgtc acccgaaagc gggagtttga aaagcgccat
     1921 agaaatctcc tagacgtgac gaatgcccac ggaccggcca tccaaacctg aattgccggc
     1981 gtcgcaagga caatgaaaat tttcacattt ggcgggaagc actggaccca atgcgaggtg
     2041 caatcggtgc ctggttctgg cgaaccgtcg ccgctctaga gaaacacctc gggcggtacg
     2101 ccgagcatga cttgcccgac cgactcgaag tgggcgccgc gtgcctggat ctgttgagag
     2161 agcgtgtgca tgtcacggaa gcggcgctcg aacgggctgc ctgtaaagat cgcgtcgacg
     2221 cccgctccct tgtaggcgaa gtccgccact tcggccgcgc cctggatcgc attggtgcag
     2281 gcgagccgca cgcgggcccg atcggctacc tcgattggcg ccacgtcgtc ggcatgcgcg
     2341 tagatcgcgg tgagcgtctc gatcaggtag gcgcgagccg aaccgagccg cgcctccgcg
     2401 cgcgccacgt cggcctgcac gaccgcattg tcggcgagcc gggcaaggcc gcgcggcgcc
     2461 tttcgggacg ccagcgtgat gaactcggac agcatcgcac gcgcgatgcc aaacgccacg
     2521 gcggcgaccc cggtcgcata gaggcattgc atggtaaagg catagagcgg gccgtgctcg
     2581 cggcgcagcg tcgggtcctc gcgtgtggag ctgaacgcct cggacacgaa caggtcgttg
     2641 acgcaataag aatccgatgc cgtgccgcac agtccgatcg tccgccaggt gtcgagcagg
     2701 gtggcctcgc ccgccggaaa cagaagcgtg cgcactgcgg gccggccgaa gcggttgagc
     2761 ctgagcgtgc cgtccgcttc gagcacgtgg cagtgcgcgc ccaaccaacg ggcgtggcgg
     2821 cagccgctgg cgaaatccca cttgccggtc aggcgatagc cggcgtcgac tgctctcgca
     2881 cgcgaagcgt tgggaggtcc ccaagccacg atactgcgcg gatcggcgaa gatcgcgcga
     2941 ttgaccccag ggtccagata ggcggcgatc agggacgaac tgttggcgac gaatgcgttc
     3001 cacgcgatcg aggcgtcatg ccgggcgagt tcctcgatcg ccgccacgta gaccgcaggc
     3061 tccgtctcgt ccccgcccgc cgagcgcggc agtaacatgc ggaacagccg cgagtcatga
     3121 aaccgctcca gtaacgcctc ggggatgcgc cgcgtgcgct cgatctcgtc ggctgcggcg
     3181 gcaatctccg tcccaagctc gcgggcgcgg gcaaccgggt ccggacgcgt ctggatgttc
     3241 atgcgccacc tcaggctggc aggccattgc ggccgaatgc ccctgcgctg tcaaccgtct
     3301 tgtcaggttc ttgatggact tacgggcgcg tcgagcattc tgcgaggttc tctttcctcc
     3361 ggtcgggaag ggttgggtga aggcaaatcg gcgaagggca gggcgatgat gcctgttatt
     3421 tttggcactg ccttgcggcc gcttgatagc gtgatcgaca aggtcgcatc ggtgtgactg
     3481 ttcgaaattc tagtggcact tcggccactc gacagcatcg cgtgccattt cggtctcaac
     3541 atcaagggga taagtgggtt gcggccgatt gtgccgtacc gacttttgat attctcgtcc
     3601 caattagttt cggggcgaat gtcctcgata tttcccatgc acaaccggcg cgacgacgcg
     3661 cttgcaatat gccgccgcgc ccaacgatca caaccgaaag aagcggttcc aatggctgac
     3721 gagattgttg cgtctattat tcccctgcat caacccaaaa aaaaggccag gagcggtacc
     3781 caacgcgccc gcgcgcaaag gcagcgcaaa cggcaaaagg caaaggcacc tgcctcgcca
     3841 aattgcgaat ctccgtcttc cgaaccattg ataccggagg gcttttcgtc gacggacccg
     3901 gtttttgccg aaccgcccgc cgcgccgccc ccgaccgtca ccttgcgcgc cgccgcgcgt
     3961 gacgaagatg ctccgtcgcg aagcatttcc ccgatcctgt tgcgggccgc tgctctcgcc
     4021 ttggccggcg tcggcatcgc catgaacgga tggttcgcgc gatcgcttgg ggcaagtgat
     4081 gcggccggat ggttgttttt cgccaccggt gtcgcctctg atcttgttgc cctcgttgtc
     4141 ccctcttgtt cggccaggct ttggcaagcc cgacaccggg ccacttcgct cgcgggatgg
     4201 gcagtgtggg cgatgacatt cgtcttcgcc gtgacggcag gcgtcggttt tgccagcacc
     4261 aacatcgcag atgtcacgct
//
LOCUS       DATLJT010001550         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2444789,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001550 DATLJT010000000
VERSION     DATLJT010001550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444789"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VM910_35430"
     CDS             complement(<1..321)
                     /locus_tag="VM910_35430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI67836.1"
                     /translation="MTGGFELAEPTPPSPRSAVRRATFVTVAGILAIIGALAGAYYFA
                     MRPVTLKIAVGPANSDDLKVVQALTQAFTQNHAYIRLRPVQTDGASASADALALGKVD
                     LAVVR"
     gene            521..814
                     /locus_tag="VM910_35435"
     CDS             521..814
                     /locus_tag="VM910_35435"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVI67837.1"
                     /translation="MAERLSGAARQSALKELPGWTEVAGREAIAKTFVFKDFNEAFGF
                     MSRVALVAEKHDHHPEWRNVYKTVEVVLSTHDAGGLTSRDIELAKAMNAIAGH"
     gene            905..1279
                     /locus_tag="VM910_35440"
     CDS             905..1279
                     /locus_tag="VM910_35440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HVI67838.1"
                     /translation="MATDYSVGFDPADRLAHDRDSVRRRFWFKLKRVAARLPFVEDLL
                     AAYYCAFDKRTPRHVQGALLGAIAYFILPFDFIPDMLPVLGFTDDAAVLATAIRLVAN
                     YVTEDHREAARAALKRGIDDQA"
     gene            1289..2485
                     /locus_tag="VM910_35445"
     CDS             1289..2485
                     /locus_tag="VM910_35445"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI67839.1"
                     /translation="MPQPAGRGDTHPVIRWLMSDARKLADPGEFLEAFAGELRRSGID
                     VSRITTGVPILHPQIFSFSGLWELGKGTSERLYRAGPEPLQAISNSPIRTAYEGGGPV
                     RCDPTAPPTPGEYAILEDLRRDGYTDYVVHAVPFADGSFKALSLATRRRTGFTAEELG
                     LFDALIPALAFNLEVQALRRTARTLLDTYVGAQSGGRVLEGQIQRGTGETISAVIWLC
                     DLRGFTDLSERLPRDTLIDMLNGYFGPMCDAVLSHGGEVLKFIGDAMLAIFPIRGDPA
                     ATCKAALVAAESAQAVLAEDNRRRAAAGLPRIDYGLALHIGDVMYGNIGSDTRLDFTV
                     IGPAVNLTARIESLCRQLGRQLLLSSDFVRAGGISANPLGRFSLKGVAADQEIFAPVS
                     PDQVPG"
     gene            complement(2497..>3559)
                     /locus_tag="VM910_35450"
     CDS             complement(2497..>3559)
                     /locus_tag="VM910_35450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI67840.1"
                     /translation="AQTKQALNANTVTLITGTIGGTYVQIGADLASVLDDGNKLRVLP
                     IVGRGSVQSVADILFLQGVDVGIVRSDTLDYLEKKGFAKDIKRQFTFVTKLYNEEMTI
                     IAPKSVRTLKDLEGKKVSVDLPNGGTFVTALTVAERIGLKADFAYIEQRIAMEKLKKG
                     ELDGVIVVGGKPYKSVSTFQNDGRFHLVTVDYDRPLQGDYLPASLTAKDYPNLIAEGE
                     TVDTIAVPAVLAAYNWAPGSERYRKLSQFVDAFFSKFPTLQNPPFHPKWKEVSLAAPL
                     AGWTRLPYAQQWLDTHGIGATARSRFDDFMNQNPAASKTAPQSQTDREALFKQFQAWQ
                     ADQTAKEGARAQVGRGKQQ"
BASE COUNT          614 a         1136 c         1132 g          677 t
ORIGIN      
        1 gcgaaccacc gcgagatcga ccttgccaag cgcgagtgcg tcggcgcttg cggaggcgcc
       61 gtcggtctgc accgggcgga ggcggatata ggcgtggttc tgggtgaagg cctgggtcag
      121 cgcctgcacc accttgaggt cgtcgctgtt ggccggcccc accgcgatct tgagggtgac
      181 cggccgcata gcgaaataat aggcgcccgc gagcgcgccg atgatggcga ggatgcccgc
      241 caccgtaacg aacgtcgccc tccgcaccgc cgagcgtggc gatggcggcg taggctcggc
      301 aagctcaaaa cccccggtca tcgatctcgt aaacaatcgt ttgaggttca aattcagtgt
      361 ccagattgcg aaagttgctg acttccgaac cgccacactg gccggcatga ttatttgtat
      421 atgctagcaa atttcttgcc tgccagaggt tacatctgcg tcatggttac cggcgatgac
      481 cacagagagg gtaaagttcc aatgaaacgg gaggtttgag atggcagagc ggctatcggg
      541 agcagcgcgg caatcggcgc tcaaggaatt acccggctgg acggaggttg ccggccgtga
      601 ggcgatcgca aaaaccttcg tcttcaagga cttcaacgag gccttcggct tcatgtcccg
      661 cgttgcgctg gtggcggaaa agcacgacca tcacccggaa tggcgcaacg tctacaagac
      721 ggtcgaggtc gtgctttcga cccatgatgc cggcggcctc acgtcgaggg atatcgagct
      781 tgccaaggcg atgaatgcga tcgccgggca ttagccggac cttgcagcgg tcacgctgag
      841 tccccaattt cgggaagcag cccgcggcga tccgccgcgc ggcggatggg acagggagca
      901 agccatggcc accgactata gcgttgggtt cgacccggcc gaccggctgg cgcacgaccg
      961 cgacagcgtg cgcaggcgct tctggttcaa gctgaagagg gtggcggcca ggctcccctt
     1021 cgttgaagac ctgctcgcgg cctattactg cgccttcgac aagcggacgc cgcgccatgt
     1081 ccagggcgcg ctgctcggcg ccatcgccta tttcatcctg ccgttcgatt tcatccccga
     1141 tatgctgccg gtgctcggtt tcaccgacga tgccgcggtg cttgccaccg caatccggct
     1201 ggtcgcgaac tatgtcaccg aggatcaccg cgaggcggcg cgcgcagcgt tgaagcgggg
     1261 aatcgacgac caggcttgag ctgtcgccgt gccccagcct gccggccgag gcgacacgca
     1321 tcccgtgatc cgttggctga tgagcgacgc gcgcaagctc gcagaccccg gcgaatttct
     1381 ggaggccttt gcgggcgaat tgcgtagatc aggcatcgac gtgtcgcgca tcacgacggg
     1441 cgtgccgatc ctgcatccgc agatcttttc cttcagcggt ctctgggaac tcggtaaagg
     1501 caccagcgag cgcctttacc gcgccgggcc cgagccgttg caggccattt cgaacagccc
     1561 gatccgcact gcctatgagg gcggcgggcc ggtgcgctgc gatcccaccg ccccgccgac
     1621 gcctggcgaa tacgccattc tcgaggattt gcgacgcgac ggctacaccg actatgtcgt
     1681 ccacgcggtt ccctttgccg acggcagctt caaggcgctg tcgctcgcca cacggcgacg
     1741 aacgggcttc accgccgaag agcttggcct gttcgacgcg ttgatccctg cgcttgcctt
     1801 caatctcgaa gtccaggcgc tgcgccgtac tgcgcgtacg ctgctcgaca cctatgtcgg
     1861 cgcgcaatcc ggcggccgtg tgctcgaagg gcagatccag cgcggcaccg gcgagaccat
     1921 ctccgcggtg atctggctct gcgacctgcg cggcttcacc gatctgtcgg aacgtctgcc
     1981 gcgcgacacc ctgatcgaca tgctcaacgg ctatttcggg ccgatgtgcg atgcggtgct
     2041 aagccatggc ggcgaggtct tgaaattcat cggcgacgcg atgctcgcga tctttccaat
     2101 tcgcggcgat cccgcggcca cctgcaaggc ggcgctggtc gcggccgaaa gcgcgcaggc
     2161 cgtgctggcc gaagacaatc gacgacgcgc cgcggccggc ctgccgcgca tcgattatgg
     2221 tctcgcgctg catatcggcg acgtcatgta cggcaatatc gggagcgaca cccggctcga
     2281 cttcacggtg atcgggccgg ccgtcaatct caccgcacgc atcgaatcgc tgtgccggca
     2341 actcggacgt cagcttctgc tgtcgtccga tttcgtgcgc gcggggggta tttcggcaaa
     2401 tccgctcggc cgcttctcgc tcaagggcgt agcagccgac caggagatat ttgcgcccgt
     2461 cagcccggac caggtaccgg gctgacgggt cgtgacttat tgctgcttcc cccggccaac
     2521 ctgcgcccgc gcgccttcct tggccgtctg atcggcctgc caggcctgga attgcttgaa
     2581 cagcgcttcg cgatcggtct gcgactgcgg cgcggtcttc gacgcggcgg ggttctggtt
     2641 catgaaatcg tcgaaccggc tgcgtgccgt cgccccgata ccatgcgtat ccagccactg
     2701 ttgcgcataa ggaaggcgcg tccagccagc gagcggcgcc gccagcgaca cctccttcca
     2761 cttcggatgg aagggcgggt tctgcagcgt cgggaacttg ctgaagaacg cgtcgacaaa
     2821 ctgcgacagc ttgcgatagc gctcgctgcc gggcgcccaa ttataggcgg cgagcacggc
     2881 gggcaccgcg atggtatcga ccgtttcacc ctccgcgatc aggttcgggt agtccttcgc
     2941 cgtcaagctc gccggcaaat agtcgccttg cagcggcctg tcgtaatcga ccgtgacgag
     3001 atggaagcgg ccgtcattct ggaaagtcga gaccgacttg tacggcttgc cgccgacgac
     3061 gatcacgccg tcgagctcgc ccttcttcag tttctccatc gcgatgcgtt gctcgatata
     3121 ggcgaagtcg gccttgagcc cgatccgttc tgcgaccgtc agcgcggtta cgaaggtgcc
     3181 accattggga aggtcgacgc tgaccttctt gccctcaaga tccttgaggg ttcgcaccga
     3241 tttcggtgcg atgatcgtca tttcttcgtt gtagagcttg gtcacgaagg tgaattgccg
     3301 cttgatgtcc ttcgcaaacc ccttcttctc gagatagtcg agcgtgtcgg agcgcacgat
     3361 gccaacgtcg acgccttgca ggaacaggat gtcggcgacg ctctgcaccg aaccgcgacc
     3421 gacgatcggg agcacgcgca gcttgttgcc gtcgtcgagc acggaggcga ggtccgcgcc
     3481 gatctgaaca taggtgccgc cgatcgtgcc ggtgatcagc gtcacggtgt tggcgttcag
     3541 cgcctgcttg gtctgggcc
//
LOCUS       DATLJT010001551         3359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_376424,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001551 DATLJT010000000
VERSION     DATLJT010001551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376424"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VM910_35455"
     CDS             <1..435
                     /locus_tag="VM910_35455"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI67841.1"
                     /translation="HAEQTRIFRRFQASFRNYDLVLSPTVPVSPFPWTQLYLEELEGA
                     RLRNYYHWLALTYVVTLVTNPAISIPCGIDEHAMPFGLQVTGRFRGDRELLDAAEAME
                     QAFANISGLGRPRPDLGKLARPAVELKSIATHPPLQAATAES"
     gene            complement(512..742)
                     /locus_tag="VM910_35460"
     CDS             complement(512..742)
                     /locus_tag="VM910_35460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67842.1"
                     /translation="MEKLIGSEAFPNGSAIELKDPRKLDAAGRLLAESGYAGQPVTCM
                     AAQDLPHHNAWHDVTVDFLTRLGIMSTLDDAG"
     gene            complement(916..2016)
                     /locus_tag="VM910_35465"
     CDS             complement(916..2016)
                     /locus_tag="VM910_35465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI67843.1"
                     /translation="MRIVICGGGAIGAAIAYFTSRGGARSTVIERHAVAGAASGKSGG
                     FLALDWCRGSPLDRLARRSFELHAELSAELGDPWGYRRLTTYGGYAAEDDTARGAGGR
                     PWLADGVAITSRLGSPETTALVEPRAFTTGLMDAAQAHGAVLRYGTVVDLLRSPSGAV
                     RGVALAGGEVVEGDAVVIAMGPWSILAARWLPLPAVLGYKGHSLVFETGGTIPAEALF
                     LEYREASGELLTPELIPRADGTTWVCAISTIVPLPVDPNDVAADEGAHARIEALCRNI
                     SPALAAAPVKMRQACFRPVTEDGLPLIGAVPGIAGAYVATGHSVWGMLNAPATGEAMS
                     ELIMDGAARHVDLAPFAPGRLPPLDPARLRGS"
     gene            complement(2448..3092)
                     /locus_tag="VM910_35470"
                     /pseudo
     CDS             complement(2448..3092)
                     /locus_tag="VM910_35470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(3157..>3359)
                     /locus_tag="VM910_35475"
     CDS             complement(3157..>3359)
                     /locus_tag="VM910_35475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67844.1"
                     /translation="STRYRSKQPWRYCNTVKFFETAPRCGALGSSEFQIHRMFARTVA
                     ETDVAHDVHWPMRPTFALAVTD"
BASE COUNT          648 a         1118 c          991 g          602 t
ORIGIN      
        1 cacgccgagc agacgcgaat tttccggcgc ttccaagcga gtttccgcaa ctatgacttg
       61 gtattgtcgc cgaccgtgcc ggtatcgcca ttcccttgga cgcagctcta tcttgaggag
      121 ctcgaaggcg cccgactgcg caactattat cactggctcg ctttgaccta tgtggtcacg
      181 ctcgtcacca acccggcaat ctccattccg tgcggtatcg acgagcacgc gatgcccttc
      241 ggcttacagg tcaccggccg ttttcgcggc gaccgtgagc tcctggacgc ggccgaggcg
      301 atggaacagg cctttgccaa catctcgggg ctcggacgcc cgcgccccga ccttggcaag
      361 ctcgccaggc ctgcggtcga gctcaaatcg attgcgaccc acccgccctt acaggccgca
      421 accgctgaat cctgactttc ggtggtcaag ttctccggcg tcaagcgaac tagaccttga
      481 tccactcgct ctcgtccgtg cacggtgtgt gtcagccggc gtcgtccaaa gtcgacatga
      541 tgccgagccg cgtcaggaaa tcgaccgtca cgtcgtgcca agcgttgtga tgcggcaagt
      601 cctgcgccgc catgcaggtg acgggctgac cggcataacc gctctccgcc agcaagcgcc
      661 ccgcggcatc gagctttcgc ggatccttga gttcaatggc cgacccgtta ggaaatgctt
      721 cgctcccaat caacttttcc acagccggaa atctgcttcc ccactgcttc ccccggcctg
      781 ttccggggaa gacagctacc tcaggaaatg cccgccccat cgcaacaact tgggggaagc
      841 acagccgcag accgttgaac cctcaaagcg gacaaagcgc agacatgctg gcatcgcaag
      901 cagcttatcg ggccttcagg agccgcgcag acgcgccgga tcaagcggcg gcaagcgtcc
      961 gggcgcgaat ggagcgaggt cgacgtggcg cgccgcacca tccatgatca gttcgctcat
     1021 cgcctcaccg gttgcgggcg cgttgagcat gccccaaaca ctgtggccgg tcgccacgta
     1081 ggcaccggca atacccggca cggcgccgat aaggggaagg ccgtcttcgg tgaccggccg
     1141 gaagcacgcc tgccgcattt tgaccggcgc cgcggcaagc gccggcgaga tattccgaca
     1201 cagcgcttcg atccgcgcgt gcgcaccctc atcagctgca acatcgttag gatcgaccgg
     1261 cagcggcacg atggtcgaga tcgcacacac ccacgtggtg ccatcggcgc gcggaattaa
     1321 ctcgggggtc aggagttcgc cgctcgcctc gcggtactcg aggaacaggg cctcggccgg
     1381 aatggtccca cccgtttcaa acaccaagct atgtcccttg tagccgagca ccgccggcag
     1441 cggcagccag cgcgcagcca ggatcgacca cggtcccatc gcgatcacga cggcgtcgcc
     1501 ttcgacgacc tcgccgcccg cgagtgctac gccgcgcact gctccgctcg ggctgcgtag
     1561 caggtcgacg accgtaccgt accgcagcac tgctccatgc gcttgagcgg cgtccataag
     1621 accggtggtg aacgcgcggg gctcgacgag cgctgtcgtc tccggtgagc cgagccggct
     1681 cgtgatcgcc acgccgtcag ccaaccacgg ccgcccacct gcaccgcgcg cggtatcgtc
     1741 ctcggcggcg taaccgccgt acgtcgtcag gcgccgatag ccccaaggat cgccgagctc
     1801 ggcagaaagt tcggcatgga gctcgaagct ccgccgtgcc aatcgatcga gcgggctccc
     1861 gcggcaccag tcgagggcca gaaagccgcc cgacttgccg gaggccgcac cggcgacggc
     1921 atgacgctct atgacggtcg accgcgcgcc gcctcggctt gtgaagtagg cgatcgcggc
     1981 gccgatcgcg ccaccaccac agatcacgat gcgcatgccc gagctcatac cgctacggtg
     2041 tcaacatcgc cacctccggg cgatgtcgct gagccaaaca gcgaggaggc gacggcggcg
     2101 ggccacggaa cgcggtattc gcgctcatgc accgccagca tctctcctta ttatgagctg
     2161 acgctatctt cacagaagtg agggtcttgt tctgagcagg tgtgagtgtc cgagtctcat
     2221 cacttggtcc gtgcagcgta aaacagccac gctgtaagct gatacgttgg tcgcaataat
     2281 ctggacggac agcaatgcgt tgcacatgca aggccatgat cactatcgcg tgccggtgct
     2341 cgtcagtgaa cgcgcaaggt cgcccgatga atacccgtgc ggtccctgtg tgcggccgga
     2401 gtggtcgctg cacacgagcc gacgatcgcc tcagagccgt cgtggtctta tagagcgctg
     2461 gccgagacca ctcggttcga cccctgcccc ggtcccagca ccaccacagt cgcgccggtc
     2521 ttcactcgac tgtacagatc aatgatgtcc tcattggtca gtcagatgca gcccgacgaa
     2581 atcgcatgtc cgatccatcc aggctggttc gtgccatgga tgcgatacag cgtgtcctgc
     2641 ccgttggcat aaaggtagag cgcgcgggcg cccattggat tagctgcacc gccttcgaca
     2701 tagtccggaa gaggtcctag ccgccgcttc tcacccgccg ttggcgtcca tcccggccac
     2761 tcctccttgc ggccgaccgt cgccacgccg gaccaggctt ggccctgctc gcccacgatc
     2821 gcgccatagc ggaccgcctt gccttggcga agcacgtagt agacgtagcg tgcgtcggta
     2881 tcgaccacga tagttcctgg cgcttctttc cggtggtaat cgacaatctg acgctgatag
     2941 gcgggtggaa tccacgcctc ctgataaggc ggatgcgcca gttgctgtct gtcgcgaggc
     3001 gtgaaattgg cgtcaaacac tggaccggac tgcatgcagc cagcaagcac cagtccagtc
     3061 aaaacaaaca agatgcctcg cgcggatgac atcgctcccc cgaagcccaa atcagggtgt
     3121 taaagtttac cttaattgtg agagtccagt ctgtgactaa tcggtcacag ccaatgcaaa
     3181 tgtgggtcgc atcggccagt gtacgtcatg cgcaacatcc gtttcagcaa cagtgcgagc
     3241 gaacattctg tggatctgaa attcggaaga gcccagggcc ccgcagcgtg gcgccgtttc
     3301 aaaaaacttg acagtgttgc aataacgcca cggttgctta ctgcggtagc gagttgaag
//
LOCUS       DATLJT010001552        12502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_146006,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001552 DATLJT010000000
VERSION     DATLJT010001552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12502
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146006"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VM910_35480"
     CDS             <1..458
                     /locus_tag="VM910_35480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI67845.1"
                     /translation="EVVEEAVMLLRHEALRHGVTTELELEWGPLPVRGDRIQLQQVII
                     NLAVNGMQAMANVRDRDRVLVMRTQRQQSDRVLVAVEDAGVGIKPENTDRLFSAFYTT
                     KHDGLGMGLSICRSIIEAHGGRVWGFSNAGPGMTFQFTVSAYGAGSVLT"
     gene            complement(479..1414)
                     /locus_tag="VM910_35485"
     CDS             complement(479..1414)
                     /locus_tag="VM910_35485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HVI67846.1"
                     /translation="MHAIGFYVSPGFQMLDLAGPAGAFEAANDQLDTPAYRLHVLAAD
                     AGAVTNSLGMATAAASLDAAVLDTLIVIGGRIDPLLSAGTLSRIAQASSRCRRVASVC
                     TGAFTLAAAGLLEGRRATTHWRYAARLQREYPSIRVEADRIFCRDGNVWTSAGITAGI
                     DLALALIEEDHGFAIAKGVAQHLVVYHRRYGGQSQFAASIDLAPKSDRMAAAVGYARD
                     HIAEVLTVERLAEAVGMSLRQFSRAFRAQTGTTPARVVERLRADLARGRIETTAESVE
                     RIAEAVGFGEAENMRRAFIRIYGQPPQSIRRAIRS"
     gene            1513..2175
                     /locus_tag="VM910_35490"
     CDS             1513..2175
                     /locus_tag="VM910_35490"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVI67847.1"
                     /translation="MPVYDGVTQLDFTGPHQFLSRTPDLDVRVASLSGQSITADHLIF
                     SDLDDLTAIDTCDVLLVPGGGGCIAVIENELFIKEIARLGAAARYLTSVCSGSLILGA
                     AGFLTGRRAACHWAWRELLPIFGAIPDDGRVVRDGNILSGGGVTAGIDFVLALIAELR
                     GQRVAEMVQLGLEYAPQPPFNSGRPDTAPAEVLEAVNVRTGPSRAQRRARLEAWATAR
                     HA"
     gene            2320..2994
                     /locus_tag="VM910_35495"
     CDS             2320..2994
                     /locus_tag="VM910_35495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVI67848.1"
                     /translation="MSGSEKPDFNHVLAIGRALDLTLPDSHAVRDAYAQAEAESPPWL
                     FNHVVRSWLYGAKLTQHRALTPDAELVAVAVLLHDLGLARGGAPDRRFEVVGADVGRA
                     FALSHDMGERRAETIWDSIALHTTASIAQHKGTNVACCQHGIACDYGGVGYQELSDDN
                     KNAILSAYPRLDMKNQLTTCLCGIAKNHPNTTRDNFIADFGIKYIPGYTRVSSVDFLH
                     QAPFAE"
     gene            complement(3443..3901)
                     /locus_tag="VM910_35500"
     CDS             complement(3443..3901)
                     /locus_tag="VM910_35500"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI67849.1"
                     /translation="MQAKTRSELLTECACFDLRRATRAVSRMYDDFLRDAGLNVTQFS
                     LLRLICVEKELSISTLGRYMVMDRTSITRALAPLERDGLIHSRSGADKRIRIVSVTNK
                     GRKLVEGAEPKWRQAQEALIETIGEDRWRAMCTLLRDTTRMVRHRAVTAN"
     gene            4059..4337
                     /locus_tag="VM910_35505"
     CDS             4059..4337
                     /locus_tag="VM910_35505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67850.1"
                     /translation="MRLRTGLREQGTHVLGLHAGPIDTDMASELTLPKVKPVDVVRQV
                     LSAIEAGRDEVLADDLTRQVKAGLSNEVGINFDPGRTVAPATLELAQV"
     gene            4371..5561
                     /locus_tag="VM910_35510"
     CDS             4371..5561
                     /locus_tag="VM910_35510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HVI67851.1"
                     /translation="MTSQRSVVFAEPVRTAIGTFGGSLKDVPAPTLGSMAIGAAVARA
                     KLRPEEVETVVMGNVVQAGTKMNPARQAAVHAGLPVTIPALTVNRVCGSGAQAIVSAA
                     QEISLGNINVAVAGGMENMDQAPYLIARGRWGYRMGDGALYDSVLRDGLNDAFSDAHS
                     GWHTEDLVEKYQVTREAQDRWALRSQQRFAAAQADGRFKSQIVPVEVPGKKEPTQFDK
                     DEHNRPDTTLETLARLKPAFRPNGTITAGNAPGLNDAASAMVLADGAWADKRGLAPTA
                     RLVFYGIAAVEPGMFGLGPVPAVKQALQRAGWDIRSIERVEINEAFAAIAIVVARELG
                     LPEDVVNIEGGAVAHGHPIGATGAVLTTKLIHSMRRDGLTRGLVTLCIGGGQGIALAI
                     EAIA"
     gene            5604..6017
                     /locus_tag="VM910_35515"
     CDS             5604..6017
                     /locus_tag="VM910_35515"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI67852.1"
                     /translation="MPVAKIHVLEGRYDKARLGDVSRAVQNALISALDIPTEDFFQII
                     HVLPRGQFLHTPSFLGLNYSDDLIVLEITFISGRPKEKRLRLLKALNEAVVSAAGIAP
                     DDLLITLYETPGENISFGRGLAQRAHISAEDPKAA"
     gene            6130..6429
                     /locus_tag="VM910_35520"
     CDS             6130..6429
                     /locus_tag="VM910_35520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67853.1"
                     /translation="METQLWRPNVGIAELGEHNLYHLGADGLEPITMVMVAFRRGRPA
                     EKRLALGRAIVDVCVELLGVPRRSVMIEFTPHVGEEILRDGNWVADWTPAEAVAS"
     gene            6490..7521
                     /locus_tag="VM910_35525"
     CDS             6490..7521
                     /locus_tag="VM910_35525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017995646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVI67854.1"
                     /translation="MSEPVVKQIVLASRPQGLPTRENFRLQEAPMTELPQGGLLLRVL
                     YLSLDPYMRGRMDDRKSYAKPVGVGEVMTGESVCEVIASDQAGYAVGDIVLAHTGWRT
                     HAASAGVALRKLDPAVAPITTGLGVLGMPGFTAYAGLNLIGKPKPGETVVVAAASGPV
                     GSLVGQLAKLSGARAVGIAGGAEKCRSVTEEFRFDAAVDHRAPDFAEKLAAACPNGID
                     VYFENVGGAVWQAVLPLLNKFARVPVCGLIAQYNAAGPAPGPNLLPATMREVLSKSLT
                     LRGYINYEFAAEHFAAFLRTVGVAVADGRIRYREDITDGFENAPAAFIGMLEGRNFGK
                     ALVRVTPSV"
     gene            7634..8104
                     /locus_tag="VM910_35530"
     CDS             7634..8104
                     /locus_tag="VM910_35530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVI67855.1"
                     /translation="MAFTIKVNDKTHSVDVDGDTPLLWVLRDVLGMTGTKFGCGMALC
                     GACTVHLDGAAIRSCITPVDSIGSSKITTIEAIGATPAGAKIQKAWLEREVVQCGYCQ
                     SGQIMSATALLASNPHPTDSDIDNAMSGNICRCGTYLRIREAIKQAAQSSGQGG"
     gene            8113..10314
                     /locus_tag="VM910_35535"
     CDS             8113..10314
                     /locus_tag="VM910_35535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI67856.1"
                     /translation="MRHLESQAVGPDPSPSLNDLSRRKFLQVGAVAGGGLMLGLRLPF
                     ANGDVEAAGAHSFTPNAFVRIQPDGQIVLTMPYVEMGQGTYTSIPMLIAEELEVDLKQ
                     VRLEHAPPNEKLYGNPLLGGIQATGNSNAVRASWQPLRQAGAIARTMLVSAAAKRWNV
                     DPASCRAQSGEVLHPPSGRRAKYGELANDAARIPVPENLALKRPEDFKLIGTPAKRLD
                     ASGKVNGTAIYGIDARPPGVKIATLAQSPVFGGRVKSVDDAAAKAVKGVRQIVQLEDA
                     VAVVADHMGAAKQGLAALKIEWDDGPHAKLNTKDIADQLEKATLNAGAVAQNIGDTER
                     AMANAVTKVEAIYQVPFLAHATMEPMNCTVHLRKDGCEIWLGSQAMARVQAAAAKTAG
                     LPLDKVVVHNHLIGGGFGRRLEADGVIRAVQIAQHVDGPVKVVWTREEDIQHDMYRPY
                     WLDRLSAGLDAKGMPVAWHNRFAGSSVASRWASGWFKNGLDPDSTEGAIDLVYALPNL
                     HVEYVRVEPPGIPTAFWRSVGPSHNVFVTESFIDELAAAAKQDPVAYRLALLDKNPRA
                     KAVLQLAAEKAGWGQPLPARVGRGVSLQFVFASYMAHVAEVEVGKDGAVRVRRVVCAV
                     DCGTVVNPDTVCAQIQSGIIFGITAALHGDITLKNGRVEQTNFDTYQVMRMNEAPAIE
                     VHIVQNFEPPGGMGECGTSAIVPAVANAIFAATGKRLRKMPVDAAALKLPV"
     gene            10418..11218
                     /locus_tag="VM910_35540"
     CDS             10418..11218
                     /locus_tag="VM910_35540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HVI67857.1"
                     /translation="MFPILAPAEIERMRRFGKARSYRAGEALVEVGKLGSGLTIILDG
                     HVDITQHDETGRRRHIVTHGPGSFMGELAQLRGRPALVDAHAQGPVEALIIPPDQLRA
                     LLIAEAELGERVMRALILRRVGLLETGAGGPVIVGRPQNGDVLRLEGFLRRNGHPYQS
                     MNPEDDADAKALIERFHVDTGQLPIVLCPGGQLLRNPTEPELARCLGLLGPIDPDRLY
                     DVIVVGAGPAGLATAVYAGSEGLSVLVLDCRAFGGQAGASARRDGDTG"
     gene            11223..11963
                     /locus_tag="VM910_35545"
     CDS             11223..11963
                     /locus_tag="VM910_35545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67858.1"
                     /translation="MGLDRFDGAESGPFVLRLSNNERAKARSIVIATGARYRRLAVAG
                     LEAFEGSSVHYWASPLEAKLCGGQEVALVGAGNSAGQAAVYLASHVAKVWLLVRGPEL
                     AASMSRYLVDRIAGLSNVEVLTQTNVTGLEGRDGVLEAIRWRQKSAEEVRAPIQHLFL
                     FIGADPNTDWLSGSGIALDAKGFVLTGGDCNESRNLLETSRRGIFAVGDVRSGSIKRV
                     AAAVGEGAQVVATLHAALAAMESKLPVA"
     gene            complement(12298..>12502)
                     /locus_tag="VM910_35550"
     CDS             complement(12298..>12502)
                     /locus_tag="VM910_35550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67859.1"
                     /translation="HQFTIDRNGITHTPTGWRRDSSGTITKGTDRDFDLDQVAEMGRR
                     LWLKHLRQKKEQVSRKKRPRLPW"
BASE COUNT         2382 a         3809 c         3877 g         2434 t
ORIGIN      
        1 acgaggtcgt cgaagaggcc gttatgctcc tgcggcacga ggcgcttcgg catggggtta
       61 ctacggagct cgaactcgaa tggggcccgc tcccggtgcg cggcgatcga atccagctgc
      121 agcaagtcat catcaatctc gcagtgaacg ggatgcaagc catggcgaac gtgcgcgatc
      181 gcgaccgcgt gctcgtaatg cgcacacaac gccaacaatc cgatcgggta ttggtggcgg
      241 tcgaggacgc cggcgtcggt atcaagcctg agaatacaga ccgactgttc agcgcattct
      301 acacgaccaa gcatgatggc ttgggcatgg gactttcgat ctgccgctcg atcatcgagg
      361 cgcacggcgg gcgggtatgg gggttctcca atgccggccc aggcatgacg tttcagttta
      421 cggtttcggc atatggggcc ggatctgtct tgacttagaa tagttacggc ggccgcttct
      481 aacttctaat tgcgcgcctg atcgattgag gcggttgtcc gtagatacgg atgaatgccc
      541 gtctcatgtt ttccgcctca ccgaagccaa ccgcctccgc gatgcgttcg acagattcgg
      601 cagtcgtctc tatgcggcct cgggccagat cagcgcggag ccgttccacc acacgcgcgg
      661 gtgtggtgcc agtctgcgcc cggaatgcgc ggctgaactg gcgcaggctc atgccaaccg
      721 cctccgccaa acgctcgact gtgagcactt cggcgatgtg atcgcgagca tagccaacgg
      781 cagcggccat ccggtcactc tttggcgcca gatcgatgga agctgcgaat tgcgattgtc
      841 cgccatagcg gcgatggtag acgacgaggt gctgtgcgac gcctttggcg atagcaaaac
      901 catggtcctc ctcgatcaaa gccaacgcaa gatcgatacc ggccgtgatg ccagctgacg
      961 tccatacgtt gccgtcccga cagaagatgc gatctgcctc aacccggatg gatgggtact
     1021 ctcgctgaag gcgcgccgca tatcgccagt gcgtcgtcgc tcgacgccct tccaataatc
     1081 cagccgccgc gagagtaaac gcaccggtgc agacactcgc aacgcgccgg catcggctcg
     1141 aggcctgcgc aatacggctc agcgtgccag cggataagag cggatcgatc cgcccgccga
     1201 tgacaatgag agtatcgagc acggcagcat cgagcgacgc ggcagcagta gccatgccga
     1261 ggctgtttgt tacggcaccc gcatcagctg cgagcacgtg gagccgatag gcgggggtgt
     1321 cgagctggtc atttgccgct tcaaacgcgc cggccggacc ggcgaggtcc agcatctgaa
     1381 accctgggga aacgtagaaa ccgatcgcat gcatggccga attataggga agttttacct
     1441 ttcggtcata cagcctcggc tgtagcttgt ctaccaggag gacaggcgat gacacagcca
     1501 atcagcgtgg tgatgccggt gtacgacggc gtgacccagc tcgacttcac cggtccacac
     1561 cagttcctct cgcgcacgcc cgacctggat gttcgagtgg cttcgctgag cggccaatcg
     1621 atcacggcgg atcacctcat cttctcggac ctggatgacc taactgccat cgatacatgc
     1681 gacgtgcttc tggtccctgg cggcggcggt tgcatcgctg tcatcgagaa cgaactcttc
     1741 atcaaggaga tcgcccggct gggagcggcc gcgcgctatc tgacctcggt ctgctccgga
     1801 tctctcattc tgggtgctgc cggattcctc accggccgcc gcgcggcctg ccactgggcg
     1861 tggcgggagt tgcttccgat cttcggtgcc atccccgacg atggccgcgt cgtgagggac
     1921 ggcaatatcc tgtccggagg cggcgtcacg gcaggcatcg attttgtgct ggcccttatc
     1981 gcggagctgc gtggccagcg cgtggcggaa atggtgcaac tcggtttgga atatgcccct
     2041 cagccgccct tcaactccgg acgcccggac acggcgccgg cggaagtatt ggaagcggtg
     2101 aacgtgcgca cggggccgag ccgcgctcaa cgacgtgcac gcctcgaagc ctgggccacc
     2161 gcccgtcatg cctgaggtcg caggcgtgcg agaccgccgg gcctttctga ccttcgtcgg
     2221 aatgcaagtc gccgcatggt cggtcccggc ttccgccatc ccgatccatt ccggaatgta
     2281 gacgaaccac gcctgtttgt gacacccatg gagcgagatg tgagcggaag cgaaaagccc
     2341 gatttcaacc atgtgctggc aatcggccga gcgttggatt tgaccttgcc cgacagtcac
     2401 gccgtccggg acgcctacgc gcaggcggaa gctgaatcgc cgccttggct tttcaaccat
     2461 gtggtgcgct cgtggctcta tggtgccaag ctgacacagc atcgcgcgct cactccagat
     2521 gccgaactgg ttgcagtggc ggtgcttctg catgatctcg gcctagcgcg cggtggcgct
     2581 cccgatcgtc gcttcgaggt tgtgggcgcg gacgttggcc gcgccttcgc cctttcgcac
     2641 gatatggggg agcgccgcgc ggagacgatt tgggactcga tcgcgctcca caccaccgcg
     2701 tcgattgcgc aacataaggg caccaacgtg gcctgctgtc agcacggcat cgcatgcgac
     2761 tatggcggcg tcggctatca ggagctgagc gacgacaaca agaatgcgat cctgtcggcc
     2821 tatcctcgcc ttgacatgaa gaaccagctg acgacgtgcc tttgcggcat tgccaaaaat
     2881 catccgaata caacaaggga caatttcatt gcggacttcg gcataaaata cattccggga
     2941 tatacgcgcg tgtcatcggt tgactttcta caccaagcac cctttgccga gtaggcgatc
     3001 agcgacgtta gtgagaaagc aacgtcgaag ggcgccgcgc cgtgggatct cggtacgtca
     3061 tcgctgccgg gtatgcgaca aagcgtccgc tttcttcccg tctcgtgctt ccgcagtgct
     3121 tccgcggcgt ttgtgcgtgc aaactaccgt tctctaagtc tttgaatctg gcgcgcctga
     3181 agagattcga actcctgccc ccccagagtc gtagattgag caagaattcc attatgtaga
     3241 attgggaatt gcgaagataa tttttcgtag ggggctggac tccgcggaag aacataattt
     3301 ttcgtagcga gctggtacga cgtgtctctc ctcctgacgc ttcatttaga cgaaacgtca
     3361 gtcagttcac cggaagcaat taaaagatct gtctttcgga ttattatttc gtcagcaagc
     3421 cagtctggtc ggggtattgt ggctagttcg ccgtgacagc ccggtgccgt accatacggg
     3481 tggtatcccg caacagagtg cacatcgcac gccaacggtc ttcgccgatc gtctcgatca
     3541 gcgcctcctg cgcttggcgc cacttcggct cagcaccctc gacgagcttg cggcctttgt
     3601 tcgtgacaga aacgatacgg atacgcttgt cagcgccgga ccgactatgg attagcccgt
     3661 cccgctccaa cggagcgagc gcgcgggtaa tgctggtccg atccatgacc atgtaacggc
     3721 caagcgtcga gatcgacagc tccttttcga cacagatcag gcgaagcagc gagaactgcg
     3781 taacgttcag tcctgcatcc cgcaggaagt cgtcgtacat gcgcgacacg gcacgcgtcg
     3841 ccctgcgcag gtcgaaacac gcgcattcag ttagcaactc actgcgtgtc ttggcttgca
     3901 tccctcatcc ccgtctcgtc cgcccaggag cggcggcagg gggcctcaca aaaaagtgat
     3961 tatacaccta atatacacgt attgcgatat cttcgagggt gccaataata cgtgcgtaca
     4021 catgcattcg tcaagttgtg acacaaggag atttacctat gcggctacgg accggcttgc
     4081 gcgagcaagg tacgcacgtc ttgggtctgc atgccggtcc gatcgacacc gatatggcga
     4141 gtgaactcac gcttccaaag gtcaagcccg tagacgtcgt tcggcaagtg ctgagtgcaa
     4201 tcgaagctgg ccgtgatgag gtgctcgccg acgacctgac ccgacaggtc aaggctggcc
     4261 tgtcgaatga ggtgggcatt aacttcgatc cggggcgcac tgttgcgccc gcgactttgg
     4321 agctcgcgca agtctgagat gcaccatctc ctcctctcag gaggtttgcc atgacatcgc
     4381 agcgttcggt tgttttcgca gagccggttc ggacggcaat cggcaccttt gggggctcgc
     4441 tcaaggacgt tccggctccc actctcggtt ctatggcgat cggcgccgcg gtcgcgcgcg
     4501 cgaagcttcg ccccgaggag gtggaaaccg tcgtcatggg caatgtcgtg caagccggca
     4561 ccaagatgaa tcccgcccgg caagccgctg tccatgccgg gctgccggtc acgattccgg
     4621 cgctcacggt caaccgggtc tgcggctcag gggcgcaagc catcgtctcg gcagcgcagg
     4681 agatttccct gggcaacatc aatgtcgcgg tggcaggcgg catggagaac atggaccagg
     4741 cgccttatct catcgcgcgc ggtcgctggg gctaccgcat gggcgacggc gcgctctatg
     4801 acagcgtgtt gcgcgacggg ttgaacgacg ctttctcgga cgcgcattcc ggctggcata
     4861 ccgaggatct cgtcgagaaa taccaggtca cccgcgaggc gcaggaccgt tgggcattgc
     4921 gatcgcagca gcgcttcgcc gccgcgcagg ccgacggtcg tttcaaatcg cagatcgtgc
     4981 cggtggaagt acccggcaaa aaggagccaa cgcagttcga caaggacgag cacaaccggc
     5041 ccgatacgac gctcgaaaca ctggcgcggc tcaagccggc cttccgtcca aacggcacga
     5101 tcaccgccgg caacgccccc ggactgaacg atgcggcctc ggccatggtg ctcgccgacg
     5161 gcgcgtgggc cgacaaacgc ggtctcgccc cgacagcacg cctggtcttt tacggcatcg
     5221 ctgcggtcga accgggcatg ttcggattgg gacctgtgcc cgcggtcaag caggccttgc
     5281 agcgcgccgg ctgggatatt cgctccattg agcgcgtcga gatcaatgaa gccttcgcgg
     5341 ccatcgccat cgttgtcgcg cgtgagctcg ggctgcccga agacgttgtc aacatcgaag
     5401 gcggcgccgt ggctcatggc catccgattg gcgccaccgg cgcagtgctg acgaccaagc
     5461 tgatccattc gatgcgccgc gacggtctga cgcgtggcct tgtcacgctt tgcattggcg
     5521 gcggtcaggg catcgcgctg gccattgagg cgatcgcata gccgcaagga agttacaaac
     5581 tccaatgatc aaaggagccg cacatgcccg ttgcaaaaat ccacgtactc gaaggacgat
     5641 atgacaaggc acgactcggc gatgtgtcgc gcgccgtgca aaatgcgctt atcagcgcac
     5701 tggacattcc gacggaagac ttcttccaga taattcacgt tctcccgcgt ggccagtttc
     5761 ttcatacgcc atcgttcttg gggctcaatt attcggatga cctgattgtg ctggagatca
     5821 cgttcatctc cggccgtccc aaggagaagc ggctgcgatt attgaaggcc ttgaacgagg
     5881 ccgtggtatc ggccgccgga atcgctcccg atgatctttt gattacgctc tacgagacgc
     5941 ctggcgagaa catctcattc ggccgaggct tagctcaacg tgcccacatc tcggccgagg
     6001 acccaaaggc tgcctagtta ggccggcgag acgttcgtct gacagcggcc tgaaggagga
     6061 ttgccatgcc ttacctccac cttgatttac ccggcaccta tcccgtcgcg gtcaagcgcg
     6121 aattatatca tggagacgca gctgtggcgg cccaatgtcg gcatcgctga gctcggcgag
     6181 cataaccttt atcatctcgg cgccgacggc cttgagccca ttaccatggt catggtcgcg
     6241 tttcgtcgtg ggcgcccggc ggaaaagcgc ctggctttgg gtcgagcaat cgtcgatgtc
     6301 tgcgtcgagc tgctcggagt gccgcgtcgc tccgtcatga tcgagttcac accgcatgtc
     6361 ggtgaagaaa tcctccgcga tggaaactgg gtcgcagact ggacgccggc tgaggccgtc
     6421 gcgagttgac atgcacacag caagccattc gatcgtccgg tcggctaaac gcgttgtcgg
     6481 gagaggacca tgagtgagcc cgtggtgaaa cagatcgtgc ttgcgtcccg gccccagggg
     6541 ctgccgaccc gcgagaactt ccgcctgcag gaagcgccga tgaccgagct gccgcagggc
     6601 ggtttattat tgcgcgtgct ctacctgtcg ctcgatcctt acatgcgcgg ccgcatggac
     6661 gaccgcaaat catacgcgaa gccggtcggc gtcggcgagg tgatgacggg tgaaagcgtg
     6721 tgcgaggtga tcgcgtccga ccaagctggt tacgccgtgg gcgatatcgt gttggcacac
     6781 accggctggc gcacgcacgc ggcctcggca ggcgtggcct tgcgcaagct cgaccccgcc
     6841 gtggcgccga tcaccaccgg cctcggcgtg ctcggcatgc ccggattcac cgcctatgcc
     6901 ggtctcaacc tgatcggtaa gcccaagccg ggcgaaaccg tcgttgtggc ggccgcaagc
     6961 ggaccggttg gctcactggt tgggcaactc gccaagctct cgggcgcgag agcggtcggc
     7021 atcgccggcg gcgcggagaa atgccgctcc gtcaccgaag aattccgctt cgatgccgct
     7081 gtcgatcatc gcgcacccga ctttgccgaa aagctcgcag cggcctgccc gaacggcatc
     7141 gatgtttatt tcgaaaacgt cggtggtgcc gtgtggcaag ccgtgctgcc tttgctcaac
     7201 aagtttgcgc gcgtgccggt gtgcgggctg atcgcccaat acaacgcagc gggtcccgct
     7261 cccggaccaa atctccttcc tgcgaccatg cgtgaggtgc tgagcaaaag tctcacgctg
     7321 cgcggctaca tcaactacga attcgccgcc gaacacttcg cggcgttcct gcgcaccgtc
     7381 ggcgtcgccg tcgccgatgg ccgcatccgc tatcgcgagg acatcaccga cggtttcgag
     7441 aatgcgcctg ccgcgttcat cggcatgctg gaaggccgca atttcggaaa ggcactggtt
     7501 cgcgtcacac cttcagtgtg acatcgttcg aagatggtcg agttctaacg ggaataaaga
     7561 gcggagttcc tactttacac agaggacgct cccgctggga aattgcacat ctgcgtagaa
     7621 aggagaggat cccatggcgt tcaccattaa agtcaacgat aagacgcaca gcgtcgacgt
     7681 cgacggggac acccctctgt tgtgggtttt gcgcgacgtg ctgggcatga ccggcacgaa
     7741 gttcggctgc gggatggcgc tgtgcggcgc ttgcaccgtg cacctcgacg gcgctgccat
     7801 ccgttcctgc atcacgccgg tcgacagcat cggcagctct aagatcacga ccatcgaggc
     7861 gatcggcgca acgccggcag gcgccaagat ccagaaggcc tggctcgagc gcgaagtcgt
     7921 tcaatgcggc tactgccagt ccggccagat catgtccgcc acagcgctgc ttgcgagcaa
     7981 cccacatccg acggattccg atatcgacaa cgcgatgtcc ggcaatatct gccgctgcgg
     8041 gacctatctg cgcattcgcg aagcgatcaa gcaggcggcg caatcgagcg gacagggagg
     8101 ctgacaatga tgatgcgtca tctcgaatct caagccgtcg gcccggatcc gtcgccatca
     8161 ctgaatgatc tctcccggcg caagttcctg caggtggggg ccgtggcagg aggaggcctg
     8221 atgctgggtc tgcgcctgcc cttcgcaaac ggcgatgtcg aagcggccgg cgcccatagc
     8281 tttacgccca acgccttcgt gcgcatccaa cccgacggac agattgtcct gaccatgcct
     8341 tacgtcgaga tgggccaagg cacctatacc tccatcccga tgctgatcgc tgaagagctt
     8401 gaggtggatc taaaacaagt gcggctggag cacgctccgc ccaatgaaaa gctctacggc
     8461 aatcctttgt tgggcggcat acaggcaacc ggcaattcca acgcggtacg cgcatcatgg
     8521 cagccgctgc gccaagccgg tgcgatcgcg cgaaccatgc tcgtatcggc cgcggcaaag
     8581 cgctggaacg tcgatccggc atcctgccgc gcgcaaagcg gtgaagtgct tcacccgccg
     8641 tcgggaagac gggccaaata tggcgaactt gccaacgatg ccgcgcgcat accggtcccc
     8701 gaaaacctgg cgctcaagcg acccgaagat ttcaagctca tcggtacgcc ggcgaagcgg
     8761 ttggatgcat cggggaaggt caacggtacc gcgatctacg gcatcgatgc ccggccgccg
     8821 ggcgtgaaga tcgccaccct tgcgcaatcg cctgtgtttg gcggtcgtgt gaagagcgtg
     8881 gacgatgcgg ccgccaaagc cgtcaagggc gtgcgccaga tcgttcagct cgaggacgct
     8941 gtcgccgtcg tggccgacca catgggggcg gcgaagcaag ggctggcggc cttgaagatc
     9001 gaatgggacg acggtccgca cgccaagctc aatacgaagg acatcgcgga ccagcttgaa
     9061 aaagcgacgc tcaacgcggg ggcggtggcg cagaacatcg gcgacaccga gagggcgatg
     9121 gcgaacgccg tcaccaaggt ggaggcgatc taccaggttc cgttcctcgc gcacgcgacg
     9181 atggagccga tgaattgcac ggttcatttg cgcaaggacg gctgcgaaat ctggctcggc
     9241 agccaggcga tggcgcgagt ccaggcggca gccgcgaaaa ccgcagggct gccgttggat
     9301 aaggtcgtgg tccacaatca tctgatcggc ggtggcttcg ggcgacggct ggaggccgac
     9361 ggcgtcatcc gtgccgtcca aatcgcgcaa cacgtcgacg gcccggtcaa ggtcgtgtgg
     9421 acgcgcgagg aagatatcca gcacgacatg tatcggcctt attggctcga ccggctttcc
     9481 gccggtctcg atgcgaaagg aatgccggtc gcctggcaca atcgcttcgc cggctcctcg
     9541 gtcgcgtcgc gatgggcatc tgggtggttc aagaacggcc tcgatcccga cagcaccgag
     9601 ggcgcgatcg atctcgtcta cgcgctgccg aacctgcacg tcgagtatgt gcgggtggaa
     9661 cctccaggta ttccgaccgc gttctggcgc agcgtcgggc cgtcgcacaa tgtgtttgtg
     9721 accgaaagct tcattgacga gttggcggcc gcggccaagc aggatccggt cgcgtatcga
     9781 ctcgcgctcc tcgacaagaa cccccgtgcc aaagccgtcc tccagctcgc cgcggaaaag
     9841 gccggctggg gacagccttt gcctgcaagg gttggccgtg gcgtctccct gcagttcgtg
     9901 ttcgcgagct acatggcgca cgtcgccgaa gtcgaggtcg gcaaggacgg agcggttcgc
     9961 gtccggcgcg tagtctgcgc ggtcgactgc ggcaccgtcg tcaatcccga caccgtgtgc
    10021 gcgcagatcc aaagcgggat catcttcggc atcaccgcgg cgctccacgg cgacatcacc
    10081 ttgaaaaacg gacgcgtgga gcagacaaat ttcgacactt accaagtcat gcgcatgaac
    10141 gaggcgccgg ccatcgaggt ccatattgtg caaaatttcg aaccgccggg cgggatgggc
    10201 gagtgcggta cttcggccat cgttccggca gtggccaacg caatctttgc tgcgaccggc
    10261 aagcgtctgc gcaagatgcc cgtcgacgcc gccgcgttga aactgcccgt gtgacgaaga
    10321 catcgtgcca aataggtgat cggccgattg ttcaccccat gaccgccttt gcaatggctg
    10381 ctggccccat tccattatcg agactcggcg tcaccagatg tttccgattc tggcgccggc
    10441 cgagatcgag cgtatgcgcc gctttggcaa agcccgctcg tatcgtgcgg gcgaggcgct
    10501 ggtggaggtc ggcaaactcg gttccgggct caccatcatc ctcgacggac acgtcgacat
    10561 cactcagcac gacgaaacgg gccgccgccg gcacatcgtt acccacgggc cgggatcctt
    10621 catgggcgag ttggcgcagc tcaggggccg gcctgctctc gtcgatgcgc atgcgcaagg
    10681 gccagtcgag gcgctgatca tcccgcctga ccaattgcgc gcattactga ttgcggaagc
    10741 agagcttggc gagcgcgtta tgcgcgcgct gattctccgt cgcgtcggtc tgcttgaaac
    10801 cggcgccggc ggaccggtca tcgtgggacg tccgcagaat ggggatgtgc ttcggcttga
    10861 gggttttctc cgccgcaatg gacatccgta ccaaagcatg aaccccgagg acgatgcaga
    10921 cgccaaggca ctaatcgagc gtttccatgt cgacaccggt cagttgccta tcgtgctttg
    10981 tccaggcggg cagctcttgc gcaatccgac cgaacccgaa cttgcccgct gcctcggctt
    11041 gctgggacca atcgatccag accgcctcta cgacgtcatc gtggtgggtg cggggcccgc
    11101 gggtctcgcc actgccgtct atgcaggttc ggaaggactc tcggtgctcg tgctcgactg
    11161 tcgggcattc ggcggtcagg ccggagcatc cgctcgtcga gatggcgata ccggatgagg
    11221 cgatggggct cgatcgcttc gacggtgctg agagtggacc tttcgtccta aggctttcca
    11281 acaacgagcg ggcaaaggct cgctcgatcg tgatcgcaac cggcgcgcga tatcggcggc
    11341 tcgccgttgc gggtctggaa gcattcgagg gctccagcgt gcactactgg gcatcgcctc
    11401 tggaagctaa gctgtgcggc gggcaggagg tggcgttggt cggcgccggc aattccgccg
    11461 ggcaagctgc cgtgtatctc gcaagccatg ttgccaaggt gtggctgctt gtgcgcggac
    11521 cagaacttgc ggcgagcatg tcgcgctacc tggtcgatcg cattgccggc ctgtcgaacg
    11581 tggaagtgtt gacgcaaacg aacgtcactg gtctggaggg tcgcgacgga gtgttggagg
    11641 caatccgctg gcgacaaaaa tcagcggagg aagttcgcgc gcccattcaa caccttttct
    11701 tattcatcgg ggcagatccg aacactgact ggctgtcggg atcaggcatc gcattagacg
    11761 cgaagggctt cgttttgacc ggcggtgact gcaacgagag ccgcaacctg ctggagacca
    11821 gccgccgcgg catttttgct gttggtgacg tacgctcagg ctcaatcaag cgcgtcgctg
    11881 cggccgtcgg agagggcgcg caggtcgtag cgacgctgca tgccgctttg gctgcgatgg
    11941 agagtaagtt gccggtcgct taacacgcct gcgcgtcgca ggcgcggagg acctctccct
    12001 cgatgctcgc cccgtcattg gacattggac gcttgagcac ccctgcttaa attcgctcca
    12061 tcgttcgtag gttggaaatt tccctgttcc ttgaaaaatt ttccctgata cgcctttgga
    12121 aattccctgt tccgttgcgt agggaatttg gctgtaagct attgaatccg cgcgtcgatc
    12181 ggacacgaaa attccctgtt aatttccctg ttagcaggga atttggtgcg gagactggtt
    12241 cgcggctgac tgcatcgcag ccaaacagtc cggtctctgt gggccatgcc tgatctgtta
    12301 ccatggcagc ctcgggcgct tttttcggct cacctgctct ttcttttgtc gcaaatgttt
    12361 cagccaaagc ctgcggccca tctctgcgac ttggtcgaga tcaaaatcgc ggtctgttcc
    12421 ctttgtgatg gttccggaag aatcacgcct ccagccggtc ggcgtgtgcg tgatgccatt
    12481 gcggtcgatt gtgaattgat ga
//
LOCUS       DATLJT010001553         8188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1986876,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001553 DATLJT010000000
VERSION     DATLJT010001553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986876"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VM910_35555"
     CDS             <1..795
                     /locus_tag="VM910_35555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HVI67860.1"
                     /translation="ESTAWPQVSRPIEYGGLGFGYKWNMGWMHDTLKYIGKDPVHRKY
                     HHGDILFGLQYAFSENFILPLSHDEVVHGKRSILGRMPGDDWQRFANLRAYYSFMFGH
                     PGKKLMFMGCEFAQVKEWTHDHSLDWQLLEQPKHAGVQTLIRDLNRLYRSTPALHELD
                     CEADGFEWIITEDASHNMFAWLRKGADPRARCVVVVNFSPNVYHNYRVRVPFAGKWRE
                     VLNSDSAHYGGTNVGNVGEVHASGGSSPELNLTIPPLAAIFLIPES"
     gene            799..2874
                     /gene="glgX"
                     /locus_tag="VM910_35560"
     CDS             799..2874
                     /gene="glgX"
                     /locus_tag="VM910_35560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HVI67861.1"
                     /translation="MRLTAGTFSRLGASWDGRGTNFALFSANAQKVELCLFDNQGRRE
                     LERIELPEKTEDVWHGYLNDISPGQLYGYRVHGPYEPEHGQRFNPNKLLIDPYARRLA
                     GRLIWSDAHFAYRAGSSREDLSFDRRDNARGMPKAVVVDETFNWGRREVRPSIPWQDT
                     IIYEAHVKGLTQLRSDVPPNLRGTYGGLSSPAMIEHLKRLGVTTVELLPIHAFVDDRV
                     LVEKKLVNYWGYNTICYFAPEGRYAQDNPLDSFRTTVARLHDAGIEVMLDVVYNHTAE
                     GNHLGPTLSFRGIDNASFYWLNRENPRYYDDFTGCGSSVNLTHPRVLQMVMDSLRYWV
                     EVCHVDGFRFDLATTLARGPNGFDRNASFLTAIRQDPVLAGVKLVAEPWDLGMGGYQV
                     GGFPSQWSEWNDRYRSAMRRYWSGEGSLIGEVSSRMTASSDLFNHDGRTQRASVNHIT
                     VHDGFTLADLFSYNQKHNEANGEDNRDGTNDNHSNNCGHEGPTDDPAILALRRQLRKN
                     QLACLILAQGLPLVLAGDEVGNSQNGNNNAYCQDNEVGWVDWQGLGREDDDLTDFIGQ
                     MIELRRKFPQIRSQRWLDGRRPDGSYGVLWLRPDAEDMKEADWKFPEGRFLAYVLAPV
                     KPETVPIFIVLNAAPEEITFKLPKLSDYKSWRQVLNTADSKKSAAEFASGADTKAPPR
                     AVLAFAGAV"
     gene            2871..4637
                     /gene="treZ"
                     /locus_tag="VM910_35565"
     CDS             2871..4637
                     /gene="treZ"
                     /locus_tag="VM910_35565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HVI67862.1"
                     /translation="MSEQQFGPRLTRNGVSFRLWAPAAQRVELMLDGPQAMQRSEDGW
                     FTAAVSGVTAGVRYKFRIDGTVEVPDPASAFQPDDVFGPSEVIDHDGFAWRALDWRGR
                     PWADTVLIESHVGAFTSDGTYRAMIDKLDHLVQTGITALELMPLADFFGSRGWGYDGV
                     LWYAPDSSYGRPEELKTLIDEAHLRGLMVFLDVVYNHFGPEGDFLHRYAPSFFTEAHT
                     PWGSAIDYRVPEVRAFAIENALAWLRDYRFDGLRLDAVNHIRSEPGAISMLHDLSVAV
                     GKFAGEKGRHIHLVLENRDNIENLLDTAQEPPRGKYRAQWNDDYHHAWHVLLTGESQG
                     YYGDYQRSPRGDLARALSSGFVYQGEVSAFWGSKRRGEPSGTLSPTAFVNFLQNHDQI
                     GNRPFGDRLESFADATAIEAALAVTLLAPMTPMLFMGEEWGSTKPFPFFCDFKGELVQ
                     AVRIGRRREYRWAYEKYGDEVPDPFDATVFQSAKLDWETRQTPRGAQRLALVQRLLSV
                     RRRNIVPRLREARFGDAQAGDSGLLTARWRMGDGATLELIANLSPHEIARPEAPMEGT
                     PIWGGEPGDHLAPWSVFWRIGT"
     gene            complement(4678..4989)
                     /locus_tag="VM910_35570"
     CDS             complement(4678..4989)
                     /locus_tag="VM910_35570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HVI67863.1"
                     /translation="MTAPLIVSIPHRLGKAEAARRLKGGLSRAAASFPIVTVDEERWD
                     GDQMFFRVRALGQAASGNIDVAEDHVRVEVVLPWLLQRFAQAAQGVIRDRGRLLLTKR
                     D"
     gene            complement(5061..7688)
                     /gene="ligD"
                     /locus_tag="VM910_35575"
     CDS             complement(5061..7688)
                     /gene="ligD"
                     /locus_tag="VM910_35575"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HVI67864.1"
                     /translation="MPLKRLSTYRKKRDFEKTAEPSGEKKVASSKRRRFVIQKHAATR
                     LHYDLRLEFDGVFKSWAVTRGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPKGEYGGGT
                     VMIWDRGFWDSNDPERGFKKGDLKFTLDGEKLHGSWVLVRMRNRGGEKRTNWLLIKHR
                     DEYAREGEANTILDEDRSSASGRAMEQIAAGKGRAPRPFMTAKSRRMNADAIWHSYRG
                     IAADARAGRAISLAVAAPDARKVSSMPDFVAPELCTAVERPPRGEGWGHEIKFDGYRV
                     QLRVEDGEVSLKTRKGLDWTDKFSAIAKEGSALPDALIDGEIVALDHEGNPNFSSLQA
                     AIADGKTGQLIFFAFDLLFADGFDLRRLPLRERKARLKALLEARKGKGASIRYVEHFE
                     ADGEAVMDSARQLALEGIVSKKLGAPYRSGRSEAWTKAKARVGQEVVIGGWKTTGGKF
                     RSLMAGVYRGDHLVFVGMIGTGFGQDTVRRIMPALKANASEESPFRGENAPRKERDVH
                     WLKPELVAEIEFAGWTADGNIRQAAFKGLREDKPAREVKAERPAMTSVTQPAVAKTAV
                     KSGRASAGKSSEVMGVVISKPDKELWPDAGDGPVTKLDLARYFEAVGEWLIAHIKGRP
                     CSIIRAPDGIKGESFFQRHAMQGTPKYLELAKVSGDRKPYLQIDRVEGLAAVAQIGGL
                     ELHPWNCAPNAYDTPGRLVFDLDPAPDVDFADVVAAAREMRQRLADVGMESFCKTTGG
                     KGMHVVVPLLHGAKEKVSWKEAKAFAQGLCQWMAGDAPDRYLLNMSKQLRKGKIFLDY
                     LRNDRMATAVAPLSPRARDGATVSMPLTWAQVKGDLDPKRFTIHTVPALLARTKAWQG
                     YEEAASSIKSAMKQLEARS"
     gene            complement(7849..8034)
                     /locus_tag="VM910_35580"
                     /pseudo
     CDS             complement(7849..8034)
                     /locus_tag="VM910_35580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
     gene            complement(8102..>8188)
                     /locus_tag="VM910_35585"
     CDS             complement(8102..>8188)
                     /locus_tag="VM910_35585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HVI67865.1"
                     /translation="RQKIETDPERPQFVLTETGIGYRLRAAD"
BASE COUNT         1512 a         2666 c         2441 g         1569 t
ORIGIN      
        1 gaatccaccg cctggccgca agtgtcacgc ccgatcgaat atggcgggct cggcttcggc
       61 tacaaatgga acatgggctg gatgcacgat acgctgaaat atatcggcaa ggatccggtt
      121 caccgcaaat accatcacgg cgacatcctg ttcggcctgc aatatgcgtt ttcggagaac
      181 ttcatcctgc cgctgtcgca tgacgaggtc gtgcacggca agcgctcgat cctcggtcgc
      241 atgcccggcg acgactggca gcgtttcgcc aacctgcgcg cttattacag cttcatgttc
      301 ggccatccgg gcaagaagct gatgttcatg ggctgcgaat ttgcgcaagt gaaagaatgg
      361 acgcacgacc attcgctgga ctggcagctt cttgaacagc cgaagcatgc cggcgtgcag
      421 accctgatcc gcgacctcaa ccggctttac cgatctacgc ccgcgctgca cgaactcgat
      481 tgcgaggcgg acggatttga atggatcatc accgaggacg ccagtcacaa catgttcgcc
      541 tggttgcgca aaggcgccga tccgcgcgca cgatgcgtgg tcgtcgtcaa tttctcgccg
      601 aacgtctatc acaattaccg cgtccgcgta ccgtttgcag gcaaatggcg cgaggttctc
      661 aattccgact ccgcgcatta tggcggcacc aatgtcggaa atgtcggtga ggtccatgca
      721 tcgggcggct cctcgcccga gctcaacctg accatcccgc cgctggccgc gatatttctg
      781 ataccggaaa gctgacagat gcgcttgacc gccggaacct tctcgcgtct cggcgcaagc
      841 tgggacggcc gcggtaccaa ttttgcgctg ttctcggcca acgcgcagaa ggtggagctg
      901 tgtctgttcg acaatcaggg gcgccgtgag ctggaacgta tcgaactgcc cgaaaaaaca
      961 gaggatgtct ggcacggcta tctcaacgac atctcacccg gacagctcta cggctatcgc
     1021 gtccacggcc cttacgagcc ggagcacggc cagcgcttca atcccaacaa gctcctgatc
     1081 gatccctatg cccggcgcct ggcggggcga ctgatctgga gcgacgcgca ttttgcctat
     1141 cgcgccggaa gttcgcggga ggatctctcc ttcgaccgcc gcgacaacgc gcgcggcatg
     1201 ccgaaggccg tcgtggtgga cgagactttc aattggggcc gtcgcgaggt tcgtccctcg
     1261 atcccctggc aagacaccat catctacgag gcgcacgtca agggactgac ccaactgcgc
     1321 agcgacgtgc cgccgaatct gcgcggcacc tatggcgggc tgtcctcgcc ggcgatgatc
     1381 gagcacttga agcggctcgg cgtcaccacc gtcgagttgt tgccgatcca tgccttcgtc
     1441 gacgatcgtg tgctggtcga aaagaagctc gtgaactatt ggggttacaa cacaatctgt
     1501 tacttcgcgc cggaggggcg ctatgcgcag gacaatccgc tggattcgtt tcgcaccacg
     1561 gtggcgcggc tgcatgatgc gggcatcgaa gtcatgctcg acgtcgttta caaccatacc
     1621 gccgaaggca atcatctcgg tcccacgctg tcgtttcgcg gcatcgacaa tgcctccttt
     1681 tactggctga accgggaaaa cccgcgctat tacgacgact tcaccggctg cggctcttcg
     1741 gtcaacctga cccatccacg tgtgctgcag atggtgatgg actcgttgcg ctactgggtc
     1801 gaggtctgcc atgtcgacgg cttccgcttc gaccttgcca ccacgctggc gcgcgggccg
     1861 aacgggttcg accgcaatgc gtctttcctg actgcaatcc ggcaggaccc tgtgctggcc
     1921 ggggtgaagc tcgtcgccga accctgggac ctcggcatgg gcggctatca ggtcggtgga
     1981 tttccctcgc aatggtccga atggaacgat cgctatcgca gcgcgatgcg gcgttactgg
     2041 agcggcgaag gcagtctgat tggcgaagtc tcgagccgga tgaccgcctc ctccgacctg
     2101 ttcaaccacg acggccgtac gcagcgcgcc agtgtcaacc acatcactgt tcatgacggt
     2161 tttacgctgg ccgacctgtt cagttacaac cagaagcaca acgaagccaa cggcgaggac
     2221 aatcgcgacg gcaccaacga caatcacagc aacaattgcg gccacgaagg cccgaccgac
     2281 gatccggcaa ttctcgcgtt gcgccgccag ctcaggaaaa atcagttggc ctgcctgata
     2341 ctggcgcagg gcctgccgct cgttctcgcc ggcgacgagg tcggcaactc gcagaacggc
     2401 aacaacaacg cctattgcca ggacaacgag gtgggctggg tggactggca gggcctcggc
     2461 cgcgaggatg acgacctgac cgatttcatc ggccaaatga tcgagttgcg cagaaaattt
     2521 ccgcagatcc gcagccagcg ctggctcgat gggcggcggc ccgatggaag ttacggcgtg
     2581 ctctggctga gaccggacgc cgaggacatg aaagaggcgg actggaaatt tccggaggga
     2641 cgatttctcg cctatgtgct ggctccggtg aagccagaga cagtgccgat ctttatcgtt
     2701 ctcaacgcgg cccccgaaga aatcacgttc aaattgccga aactgtccga ctacaagagc
     2761 tggcgacaag tgttgaacac ggccgactcc aaaaagagcg cggccgagtt tgcctcgggc
     2821 gccgacacca aagcgccgcc gcgcgcggtg ctggcctttg ccggcgcagt atgagcgaac
     2881 agcaatttgg tccccggctg accaggaacg gcgtgtcctt tcggctgtgg gcgccggcgg
     2941 cacaacgcgt cgagctcatg ctggatggcc cgcaggcgat gcaacggagc gaagacggct
     3001 ggttcaccgc cgctgtttcc ggcgtaaccg caggcgtgcg ctacaaattc cggatcgacg
     3061 gtacagtcga ggtgccggac cccgcctccg catttcagcc cgatgacgtg ttcggtccga
     3121 gcgaggtgat cgaccatgac ggctttgcct ggcgcgcgct tgactggcgc ggccggccgt
     3181 gggccgacac cgtcctgatc gaaagccatg tcggcgcctt tacgtctgat ggtacctacc
     3241 gggcgatgat cgacaagctc gatcatctcg tccaaaccgg gatcaccgcg ctcgaactga
     3301 tgccgctggc ggatttcttc ggaagtcgcg gttggggcta tgacggcgtg ctgtggtacg
     3361 cgcccgacag cagctatggc cgtcccgaag agctgaagac gctgatcgac gaggcgcatc
     3421 tgcgcgggct catggtcttc ctcgacgtgg tctacaacca cttcggcccc gaaggagatt
     3481 ttctgcaccg ctatgcgccg agcttcttta ccgaagcgca cacgccatgg ggcagcgcca
     3541 tcgactaccg cgtccccgag gtgcgcgctt ttgccatcga gaacgcgctc gcctggctgc
     3601 gcgattatcg cttcgacggc ctgcggctcg acgcggtcaa ccacatccgc agcgaacccg
     3661 gcgcaatctc gatgctgcac gatctcagcg tcgccgttgg aaaatttgcc ggggagaagg
     3721 gccggcacat ccacctcgtg ctggagaacc gcgacaacat tgaaaatctg ctcgatacag
     3781 cgcaggaacc gccgcgcggc aagtaccgag cgcaatggaa tgacgattat catcacgcct
     3841 ggcacgtgct cctgacaggt gaaagccagg gctattatgg cgactatcag cgctcgccgc
     3901 gcggcgatct ggcgcgcgcc ctgtcctcgg gcttcgtcta tcaaggcgaa gtttccgcgt
     3961 tctggggcag caagcggcgc ggcgagccga gcggcactct ttcgcccacc gctttcgtca
     4021 actttctgca aaaccacgac cagatcggaa accgcccctt cggcgaccgg ctggaaagct
     4081 tcgccgacgc aacagcgatc gaagccgcgc tcgcagtcac tctgctcgct ccgatgaccc
     4141 cgatgctgtt catgggcgag gaatggggat cgacgaagcc gtttcccttc ttctgcgatt
     4201 tcaaaggcga actcgtccag gccgtccgca tcgggcgacg tcgggaatac aggtgggcct
     4261 acgagaaata tggcgacgag gtcccggatc cgttcgatgc gacggtcttt caatccgcaa
     4321 agctcgattg ggaaacacgg caaacaccgc gcggcgcaca gcggctcgcg ctggtgcaac
     4381 gattgctttc ggtccgccgg cgcaacatcg taccccggct tcgagaggcc agatttggcg
     4441 acgcgcaagc cggtgacagc ggtctcttga cggcacgctg gcggatgggt gatggcgcca
     4501 cgctggagct gatcgccaat ctgtcgccgc acgaaatcgc gcggcctgaa gcgcccatgg
     4561 agggaacgcc gatttggggc ggtgagcccg gcgatcatct tgcgccctgg tcggtattct
     4621 ggcgcatcgg aacatgacgt gccccgggcc atttccgatc gcctgcaacc acgtgactca
     4681 gtcccgcttg gtcagcagca gcctgccgcg gtcgcggatc acaccctggg ccgcctgcgc
     4741 aaagcgctgc aacagccagg gcagcacgac ttctacgcgc acatgatcct cggcgacgtc
     4801 gatgttgcct gacgcagcct gtcccagcgc acggacgcga aagaacatct gatcgccgtc
     4861 ccagcgctcc tcatcgacgg tcacgatggg aaagcttgcg gccgcccgcg acagacctcc
     4921 cttgagacgg cgcgccgcct ccgccttgcc gaggcggtgc gggatggaaa cgatgagcgg
     4981 tgcggtcatg tgactggccc tcaacccggt ctatggcatc cagtttctca cggcaaaagg
     5041 aaaccacgcg cgaccgaccg tcacgacctg gcctccaatt gcttcatcgc cgacttgatc
     5101 gatgatgccg cctcctcata gccctgccag gcctttgtcc tggcaagcag cgccggcacc
     5161 gtatggatgg taaaacgttt gggatcaagg tcgcccttca cctgcgccca ggtcagaggc
     5221 atcgagacgg tcgcgccatc gcgcgcccgc ggcgacagcg gggcgaccgc ggtcgccatc
     5281 cggtcgttgc gcagatagtc gaggaagatc tttcccttgc ggagctgctt cgacatgttg
     5341 aggagataac ggtcgggcgc gtcgccggcc atccattggc aaagcccctg cgcaaaggcc
     5401 ttggcttcct tccagctcac cttctccttc gcaccatgga gcagcggcac gacaacatgc
     5461 atgcccttgc cgccggtggt cttgcaaaaa ctttccatgc cgacatcggc aaggcgctgc
     5521 cgcatctccc tggcagcggc gaccacgtcg gcaaagtcga catccggagc ggggtcgaga
     5581 tcgaacacca gccttcccgg cgtgtcgtag gcattgggcg cgcaattcca gggatgcaac
     5641 tcgagcccgc caatctgcgc gaccgcagca aggccttcca cgcgatcgat ctgcagatag
     5701 ggcttgcgat cgcccgagac tttcgcgagc tcgagatatt tcggcgttcc ctgcatcgcg
     5761 tggcgctgaa agaaggactc gcccttgatg ccatcagggg cacggatgat cgagcaaggg
     5821 cgtcctttga tgtgcgcgat caaccattca ccaaccgctt cgaagtaacg cgcaaggtcg
     5881 agcttggtca ccggaccgtc gccggcatcc ggccaaagct ccttgtcggg ctttgaaatg
     5941 acaacgccca tgacttcgct ggatttgccg gccgacgctc tgccggattt gactgcagtc
     6001 ttcgcgacgg caggctgggt tacgctggtc atcgcgggcc tctctgcctt cacttcgcgc
     6061 gcgggcttgt cttcccgcaa tcccttgaac gccgcctggc gaatattgcc gtcggcggtc
     6121 cagccggcaa attcgatctc ggcgaccagc tccggcttca gccagtgcac atcgcgctcc
     6181 ttgcgtggcg cgttttcgcc gcggaacggg ctttcttccg aggcgttggc cttcaatgcc
     6241 ggcatgatcc gccgcaccgt atcctgaccg aagccggtgc cgatcatgcc gacaaagacg
     6301 agatgatcgc cgcgatagac gcccgccatc agcgaacgga atttgccgcc cgtcgtcttc
     6361 cagccgccga tcacgacctc ctgtcccacc ctggctttgg ccttggtcca ggcttcagag
     6421 cggccggaac ggtaaggtgc acccagtttt ttcgagacga tgccttcgag cgcgagctgg
     6481 cgcgcggagt ccatcaccgc ctcgccgtcg gcctcgaaat gttcgacata gcgaatgctg
     6541 gcgcccttgc ccttgcgcgc ttcgagcagc gctttcaggc gcgcctttcg ctcgcggaga
     6601 ggcagccggc gcaggtcgaa accgtcggcg aacaacaggt cgaaggcgaa gaagatgagc
     6661 tggccggtct tgccgtcggc aatcgcagcc tgcaggctgg agaagttcgg attgccctca
     6721 tgatcgagcg cgacgatctc gccgtcgatg agggcgtcgg gcagcgcgct gccttccttt
     6781 gcgattgcgg agaacttgtc ggtccaatcc agccccttgc gagtcttcag gctgacctcg
     6841 ccatcctcga cacgaagctg cacgcgatag ccgtcgaact tgatttcgtg gccccagccc
     6901 tcgccgcgcg gcggacgttc caccgccgtg cagagctccg gcgcaacgaa atcgggcatc
     6961 gaggagactt tccgcgcgtc aggcgcggcg accgccaagc tgatcgcccg tccggcgcgt
     7021 gcgtcggcgg cgatgccgcg gtaggagtgc catatcgcat ccgcgttcat ccttcggctc
     7081 ttagccgtca tgaacggcct gggtgcccta cccttgccgg ccgcgatctg ctccatcgcc
     7141 cggcccgatg cggacgaacg gtcctcatcg agaatcgtgt tggcctcgcc ctctcgcgca
     7201 tactcgtcgc gatgcttgat caaaagccag ttggtgcgct tttcgccgcc gcgattgcgc
     7261 atgcgcacca gcacccagct tccgtgcagc ttctcgccgt caagcgtgaa cttgagatcg
     7321 cccttcttga agccgcgctc cggatcgttc gaatcccaaa agccacgatc ccagatcatg
     7381 acggtgccgc cgccatactc gcctttcggg atagtgcctt cgaagtcgcc gtaatcgagc
     7441 ggatgatctt ccacctcgac cgccaaccgc ttgtcgtgcg gatcgagcga cgggccgcgc
     7501 gtcaccgccc aggacttgaa gacgccatcg aattcgagtc tgaggtcgta gtgcagccgg
     7561 gtcgccgcgt gtttctggat cacgaagcgc cgtcgctttg acgaggcgac cttcttctcc
     7621 ccggatggct ccgcggtctt ctcgaagtcg cgcttcttgc gataggtcga cagtcgcttc
     7681 agtggcacaa gcgtttccgg tccgttccaa tgggcgcacc ctatcacgcc ctcccggacg
     7741 cccggaacca aaacccccga gcatctgttg aagttccagg aattgacaaa gccccctcga
     7801 gcttctggag gggctcgccc tcgtcttccg ctacttggtg ctcttgcgct tcttcgaact
     7861 cttcctgggc accttggcgc ctcgttttcg cgcctccgac agtccgatcg caatcgcttg
     7921 cttgcggctc ttcaccttcc gcttcgagcg accgcttctc aatgtccccg ctttgcgttt
     7981 gcgtatcgcg cgaccaacgt tggcggatgc cttcctcgaa tatctgcgtg ccatgcaaat
     8041 cctcccgcta gcgagagtcc aatcttcgca gcatgccaaa gttccaggcc tgcaacttca
     8101 ctcaatccgc cgcgcgcagg cgatagccga taccggtttc ggtaagcacg aattgcgggc
     8161 gctcggggtc ggtctcgatc ttctgccg
//
LOCUS       DATLJT010001554        13161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4055182,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001554 DATLJT010000000
VERSION     DATLJT010001554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4055182"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VM910_35590"
     CDS             complement(<1..739)
                     /locus_tag="VM910_35590"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVI67866.1"
                     /translation="MTAGDHAHAEGNLDASYTISFARIPVGEITATVVFGQNEYAISA
                     RARAGSVLKALLVDGEASFSTDGTINGDYPVPTTFTSKIVSNAESSDVTMVLEEGNVK
                     ELAVTPPPSSDLVPVTKANRQGIADPLTALLFSAAAAGEGLSQEACRRTLPIFDGHQR
                     YDLKLAFKRMDKMTAEKGYAGPVVVRSVNYEPIAGHRANIPLVKYLSEGREIEIALAP
                     IAGTRLLAPFRLSVVSTLANLVIEANRF"
     gene            complement(1264..1950)
                     /locus_tag="VM910_35595"
     CDS             complement(1264..1950)
                     /locus_tag="VM910_35595"
                     /inference="COORDINATES: protein motif:HMM:NF042863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HVI67867.1"
                     /translation="MFVSPAVWKACGGPVGSLAGLMLYGGLDLSEVADLTALVLIGWR
                     DGKWHVQPTFWLPSEGLSEKATADRLPYDLWRTQGYLQTTPGRTVSYEHVADHLRGLF
                     RHYNIAKIGFDRWNMRHFLPWLLKAGFNEQFVKDHFVEFGQGMQSMSPALRDLEQVLL
                     EGHLAHGDHPVLSMCAANTVIAIDDAGNRKPSKKRSVGRIDGMVALAMAIGVAPLRAQ
                     PVIDIEALIG"
     gene            complement(2231..2818)
                     /locus_tag="VM910_35600"
                     /pseudo
     CDS             complement(2231..2818)
                     /locus_tag="VM910_35600"
                     /inference="COORDINATES: protein motif:HMM:NF015321.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2811..3236)
                     /locus_tag="VM910_35605"
     CDS             complement(2811..3236)
                     /locus_tag="VM910_35605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67868.1"
                     /translation="MPRRSAASLGVVPVLPGRGRPEPPEDLDELEQRIWREVVDALPG
                     HWLDTAGQVILRRLVAQAAVSERQELRLRQLRAQQQDDGEEAGALAAQHGVVAKNVAY
                     LLSQLRATPRSRVVPRAAGSRLEQAPDSRPWEIKAKFSA"
     gene            3420..3686
                     /locus_tag="VM910_35610"
     CDS             3420..3686
                     /locus_tag="VM910_35610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67869.1"
                     /translation="MARIDGPAIDADSRVKAPPFDALLAAGVTSSTERLQRSENELIR
                     IAVMRLDVISARGRNYLARGQAHPAERFKLQLQLGPSAPVFGSM"
     gene            complement(3847..4068)
                     /locus_tag="VM910_35615"
     CDS             complement(3847..4068)
                     /locus_tag="VM910_35615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67870.1"
                     /translation="MSPLRRGLILGFMRAKQQSRKELRTLAQSFDDELLALQHEFHEL
                     AVAHYRQCYAAAVDEALIERSARPGMLLH"
     gene            complement(4773..5174)
                     /locus_tag="VM910_35620"
     CDS             complement(4773..5174)
                     /locus_tag="VM910_35620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67871.1"
                     /translation="MPIEEQVIWECFVMRRDRDLERLWQKQQAGDSLAGKFFDGLGEW
                     RAKCRKGSDKAPQCLTCEHAFCDPAQPPLTFVVTHTEDPRVNQIILTGVCWRCAEKSD
                     TQLRKHSIALLAKYIEIRTLGECDPPTQITH"
     gene            complement(5189..6814)
                     /locus_tag="VM910_35625"
     CDS             complement(5189..6814)
                     /locus_tag="VM910_35625"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase family protein"
                     /protein_id="HVI67872.1"
                     /translation="MSEQYASKTSPDDFDGEAISRADREFLAKLSDHPLMRNPLYSVP
                     PPPLPDYAALALTASAAAVEAAQRACFVAALEWMLVNYAFCKSAFMGKGGVISLVDGE
                     LGSIASLRGFMLPYALTVEGPRGGRSRTISVVDDWMKHPLRVHIDKIQTRSDRPRPTF
                     EEEGLHIYNRYWPPAHPTSGGEIATFKAFFARLIPDDTEREWMWNYLAHKARKPWVPM
                     IAVIMVAEEFGTGRGTLFEILERLFGEDYVVPCTFGEITGTAAGARFNDRLANALIAT
                     VNEAADEDGHHQARRRLNYEALKNAIEPSPTARHRFEAKGQHAYAQRSARTIMIATNH
                     RDVVKLPPTDRRFCVIACGSKMTLIERADIRAWMGVPENIGALHRALLMRPAVPLDVF
                     DPYGDPPPFAGRLKMIGMGETRLEDAYGTAIDALDGCPLFTMTQMQRLIAYFGDFKTG
                     DWSDKARHTVAKNAYRLRERSEPNNRIKYRKRQEIIYARTNADQLRWRGVDTELIIRR
                     LDMAEERVTQVVNAERDVLADLIRTQSLNSSSE"
     gene            complement(6780..7772)
                     /locus_tag="VM910_35630"
     CDS             complement(6780..7772)
                     /locus_tag="VM910_35630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67873.1"
                     /translation="MKKPLQERTAFRLAMLANGFQPLLTDCKRPIEKGWPKRTVDEAE
                     VLSWDRSALLSTGMRLDGDLAVIDVDVADAGLVAALADALGKNFPALFEHGLVRHAGG
                     PKEAWIARVDEPFRRLASRRWYRGDDPDDPAVPKHQVECFGSLGTRQFAVDGPHARNQ
                     AGEVMDVYQFAGGASPATTSRTALPALPKAAYALACDLFDEIAAAAGLVAVSKGGRGE
                     AELRCFELDADTEIETRDHGVMTVAELECHMRARRNGAATMRCSGSFHDRTRVRTDSH
                     LINWGRHGLGIYDTMTETTWHRRERAPPELFTFLEQLRERNPFNERTVCLQDEP"
     gene            complement(7778..8248)
                     /locus_tag="VM910_35635"
     CDS             complement(7778..8248)
                     /locus_tag="VM910_35635"
                     /inference="COORDINATES: protein motif:HMM:NF040299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6011 domain-containing protein"
                     /protein_id="HVI67874.1"
                     /translation="MTAETRARSLATRRRRRERKIARITKDYLLGKHIGNGSECAICG
                     RALSDPLSIARAIGSECWPRFQDHLAREVPRCAANMEQIQQAIVEHEKHDLAYWQMLN
                     AQRYSSIASDKINEFAAYDLRQTRTLKRQLRDTELLLAAARKVSGNADVIVNRE"
     gene            complement(8440..9300)
                     /locus_tag="VM910_35640"
     CDS             complement(8440..9300)
                     /locus_tag="VM910_35640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HVI67875.1"
                     /translation="MPAAIITNGALWGRAFIPWQKRHFRPRDPFGREWVALPGAEAKI
                     AAAFGTVAMTVADADMPDLPPLNVVETHVALPAEVMARYREMARELFTTAEGRTIEAA
                     SPLIATGKLAQMANGFLYDAGNEDAVFVHDLKIQWLKELVESLEGEPLLIAYEFIEDL
                     RTIQRALKWWWEVPVLGGPTSAADSALLVEAWNAGRLPLLAFHFHPAAAGYGINLQYG
                     GSRMAWLSPSWSAELTQQAIARIYRPGQTRHVTIHVCVATRTVDEMKRNRVLGKMSAQ
                     EAFKQHLEQI"
     gene            complement(9339..9755)
                     /locus_tag="VM910_35645"
     CDS             complement(9339..9755)
                     /locus_tag="VM910_35645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67876.1"
                     /translation="MNAPFAARVLRSYQQRAATWLYEHDGAFMVAPLGAGKGAAALTA
                     LADLVRDGHRRHALVIAPKLVATTVWPLEVTLWPHLEHLRVAVLNGTPERRRALLATA
                     PEREVTVIGIDLTQWLVEELAAFPNGHPGASARAGA"
     gene            complement(9752..11896)
                     /locus_tag="VM910_35650"
     CDS             complement(9752..11896)
                     /locus_tag="VM910_35650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67877.1"
                     /translation="MFEADDLIWTDFEVYGGALDLKAAGTYRYVAEASTRAIVLAYAI
                     GNAPALTWHADGAILDWDHAPDELRAAFERNATFAAWNASFDAAVWNYATLGFPLLAP
                     ERVIDPMIQAGVSNLPTDLEHASRYLGGAGKQKDGKALIRMFCIEGASPREYPAEWER
                     FLAYARQDIEAMREVYGRTRPLPSEEWRQYWAFEHINQRGVAIDMPFVQHAATLAAQD
                     AIAINRRLVELTGGAVERVTQAGRLATWMCDMLVEAPMREVLIVGTSDDDDDAELELS
                     LTRDRVERVLAMLDAKRANGGPSPSEVKAHEAATLRVYGAGAAPKKFARLKAQQLDGM
                     LRGQYRFAGAYQTGRMTSRGAQIQNLTRDVLGEDGAAESPLVEAIAKGCDYATLVAAD
                     PVDMPATRKLALIVRPALIAELGKLFVWSDWSAIEARITPWLAASKGAENVLEIFRAN
                     DRDPTRPDIYTVAIADILHKDAHAIVKSERQIGKVATLALGFGGAVGALLAMALGYRI
                     HLETAEARHIVDAWRAANPWAQEFWSGLWEAAMSAWEIPGRITTAGRLAFIYRDDYLG
                     GALFMALPSGRLLTYPRLRWREVDVRKDGKPTGEKRTELSFRRAHGRARLWHGTLCEN
                     AVSGTAADILRATVTRIETNPALAFMPIRMTTHDEIVCEISAARADEAKAILRREMLT
                     LPNWADGLPLQSEEQSCRRYSKSKTTLKGEAS"
     gene            complement(11981..12739)
                     /locus_tag="VM910_35655"
     CDS             complement(11981..12739)
                     /locus_tag="VM910_35655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67878.1"
                     /translation="MSDDHDHDRDRDHANDNGEHERPGSKQVAPAPAAGGALTSLAAL
                     QTALAKINTTVILGRTGLPMMLFKSREGSGTWGFGQKRTIPEEGSRWAVNPLTFKYGC
                     ICFNGNQVVDERLVPIDQPKPLVAEMPDTGFPWQEEWAVNMKCLDGADAGVEVVFKAT
                     TVGAIQCLVQLLDQVRDRLNSGQHDSKIVPIEVLEKDSYQHVQYGRVWTPVMNTVGWM
                     SLDGPAPAPTPATPAPTSGSAASPTDQPRRRRVA"
     gene            complement(12736..>13161)
                     /locus_tag="VM910_35660"
     CDS             complement(12736..>13161)
                     /locus_tag="VM910_35660"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI67879.1"
                     /translation="AYLQLLADGLNLVDAVKDIHTALRDQAKRALEDGDLVPGYTLSA
                     GRAARCWLNNESTTIAALESLGLDRDDVVAKTLHSPKQVELRAKARGLKIPQELIVSN
                     RSGVSLVRVENVHAPTPGRVETARAFSEALKAFQGGRQA"
BASE COUNT         2402 a         4175 c         3945 g         2639 t
ORIGIN      
        1 cgaatcgatt ggcctcgatc actaagtttg cgagcgtgct cacaacggat agccgaaacg
       61 gcgccaatag gcgcgtgccg gcgattggtg cgagcgcgat ctcgatttcg cggccctcgg
      121 aaagatattt gacaagcgga atattcgcgc gatgcccggc gattggttca tagtttacgg
      181 aacgcacgac cacgggtcct gcgtatccct tttccgctgt catcttatcc atgcgcttga
      241 atgcgagctt caaatcatag cgttggtggc catcgaagat cggtagtgtg cgccggcacg
      301 cctcttgaga cagaccttcg cctgcggcag cggccgaaaa cagcaatgcg gtcaacggat
      361 ccgcgatacc ttggcgatta gccttggtca ctggcacgag atccgagctc ggcggcggtg
      421 tgaccgccag ctccttcaca ttcccttcct cgaggaccat cgtcacatcc gagctctcgg
      481 cgttcgagac gatcttagag gtaaaagtcg tcggcaccgg ataatcgcca ttgatggtgc
      541 cgtccgtgga gaacgaggct tcgccgtcca ccaacaatgc tttcaggacg ctaccagcac
      601 gcgcgcgcgc agaaattgcg tattcgtttt ggccgaagac cacagtggcc gtgatttcgc
      661 caactggaat gcgcgcgaac gagattgtat agcttgcgtc gaggtttcct tcagcgtgtg
      721 catggtcgcc ggctgtcacc gttatcaacg cgagtgcgaa gcctgcggcg agaatcgttg
      781 tttgagcggc gcgagaacga aacctcatct tggaatccgt cccttggcct acagccgatt
      841 atgctttgac ctaggcccct ccgaccgaaa ggatagcaca cctctgcaag gccacagact
      901 gctgcgctgc gggttttcgg tccggcctat gacggcttcg ggtccaaaac cggtagtcct
      961 ctggtcgagc acaagatttc cgcttcgtcc ccaaaacctg acatctgcgc gttaatgagt
     1021 acgcgcctta acacctcatc tcgcgtcggg agtggccctc ggctccgaca ctggcgcctc
     1081 aaaatattcc tgggcaacta gcggctccgc atcgatcgcc gacgactgct tgcaacaact
     1141 acgaggtaaa aagcgcagcc tccgatcgtc cggtccatgc cggcagcata aacagggaag
     1201 cagttggatc acgcgagtag ccatggcggc tccatagagt gacatagcgc ttgcccagcc
     1261 ccgttaccct atcaaagcct caatatcgat gacaggctgc gccctcaacg gtgccacccc
     1321 aatcgccatc gcaagcgcaa ccatgccgtc gatccgacct accgaacgct tcttcgacgg
     1381 cttgcgatta ccggcgtcgt caatcgcaat caccgtgtta gctgcgcaca tcgagagcac
     1441 cgggtgatcg ccatgggcga gatggccctc gagcaacacc tgctcgagat cacgcagcgc
     1501 gggcgacatc gattgcatcc cttggccgaa ttcaacgaag tgatccttga cgaactgctc
     1561 gttgaagccg gccttgagca accacggtaa gaagtgccgc atattccagc gatcgaatcc
     1621 gatcttcgca atgttgtagt gccggaatag gccacgcaaa tgatcagcga catgctcgta
     1681 cgaaacagtc ctgccgggcg tcgtctgcag ataaccttgc gtccgccaga gatcatacgg
     1741 aagccgatcg gctgtcgcct tttcgctcaa cccctccgat ggcaaccaaa atgtcggctg
     1801 cacatgccac ttgccatcgc gccatccgat taaaaccaac gcggtaaggt ccgcgacctc
     1861 ggataaatcg agaccaccgt agagcatcag acccgcaagc gaaccgaccg gcccgccgca
     1921 agctttccaa accgccggtg aaacgaacat gtgcgtgggt gggttcgacg cgctgattca
     1981 aaatcaagtt gcgatattca gcttcgcgcg ccggcatgcg cttggcagcc gctgccatag
     2041 caagaacttc ctgcgtgttc aggaacgtgc cgagtgccgg attagccaag gcgatgatgg
     2101 tttcgttgaa cgggtcgagg tcagcgggcg cagtatagag cttaactacc gtgtgcggat
     2161 cgtgtccggc caacgcgtca tcgatcaaca ccgagaacag gtcagcatcg gtcggtgcct
     2221 gcgttgagat gatgatcgag agcggattct cctgcgcgcc ggtcgccgtc tccagcgcct
     2281 catacaatgc agagcgcgga ccacggacct gacccagctc gtcatggatg accaattggg
     2341 gagatagtcc atacgccgtc gttgcgtccg ccgagagcgc gcgatagcgc gtgccgagct
     2401 cggtgcagat gagcgatttc gcggtttcct gaatcgtcac gatccgcgcc agcgccgggt
     2461 ttatgcgcac catcttcgca gccaaagaga agatgatgcc cgcttgatcg cgcgactgcg
     2521 ccgcggagaa cagctgagaa ttcggcttac tctttgcagg gggcccacac aaatgcgcga
     2581 gcaataggca cgcagccagg gtcgtttttg cattcttgcg gcccatgctg ataatcgcac
     2641 gacgggtgcc gtgcgggttg ttgtaaatca gacgaatcaa atctttctgc caatcgaaca
     2701 gctcgagccg cttgccgacg agcctgccct cggggatgaa gcagacggtc tcgatgaact
     2761 cgatgacgtc ggccgcagtg accttgtcgt cagccggctt ccgcttgcgt ctaggcactg
     2821 aacttcgcct ttatttccca cggtctggaa tccggagcct gctcgagccg cgaaccggcc
     2881 gcacgcggca ccacccgcga acgcggcgtg gcccgcagct ggctcagaag atacgcaacg
     2941 ttcttggcca ccaccccatg ctgggcagct agagcgccgg cctcctcgcc atcgtcctgc
     3001 tgctgagctc gcaactgccg cagccgaagt tcctgccgtt ccgagaccgc ggcctgcgcc
     3061 accaggcgac gaaggatgac ctggccggca gtatcgagcc agtggccggg cagcgcgtcg
     3121 acaacctcac gccaaatccg ctgctccagc tcgtcgagat cctccggcgg ctccggacga
     3181 ccacgcccag ggagcacagg cacaacgcca agcgaagcag cggatctgcg cggcatgtgg
     3241 ggcacattag cacagcccga aaatcatgca cgttagcggt gcccggccgc gccgctggtc
     3301 gggaattgcc gccctttcga atttttccca cccccctggc ccccggtttt cttacccagg
     3361 ggaaggaatc catgggtcgc gcttcaccgg cctccgcgcc cgggttaccg attggctgga
     3421 tggcgcggat cgacgggcca gccatcgacg ccgacagccg tgtcaaagcc ccgccgttcg
     3481 atgctctgct tgcggccgga gtgacaagca gtacagagcg actgcaacgg tccgagaatg
     3541 aacttattcg catcgccgta atgcggctcg atgtgatcag cgcacgtggc cggaactatc
     3601 ttgcccgcgg ccaggcacat ccggcagagc ggttcaagct tcaattgcag cttggccctt
     3661 cggcgccagt attcggttcc atgtagacgg cccatcgggt tcctcctgct gcgcctatgt
     3721 aacacttacc gagttagtca tccggctaac tcaggagcta ccggccgggc caaggtgggg
     3781 tcaacgcatc gaagccccca accccagccc cgaccgagcg gccgtgtgcg tttccggccg
     3841 ctatggctag tgcagcaaca tcccgggacg tgcgctgcgt tcgatgagcg cctcgtcgac
     3901 ggcggcggcg tagcattggc gataatgcgc gaccgcgagt tcgtggaatt cgtgctgcaa
     3961 agccagaagc tcgtcgtcga aggactgggc cagggtgcgc aattccttac ggctctgctg
     4021 cttggcgcgc atgaagccga gaatgaggcc acgccgcagt gggctcaatt gccccgtctc
     4081 atggttttgg cggctctttg gcgttaggtg ggccatcgcg cttaaccccc gacacgggag
     4141 aacctcgcag ctctagctcg attaagatat cgaatcagcg ccggcgcccc ttcgagcgtg
     4201 cccacacgct caatgctttt agcacaagcc cttagcccgc gcaccaggcc ctcggcatcc
     4261 tcaaagtcgg ggtcgccgcc aaactcgcgc tcaatccgat cggcatagac gcgcatttcc
     4321 gccgcgcgcc tcagaacgca ctccgcgatc agagggagga ggtcgacaac gttagactcg
     4381 tccatgcctt catctcagca cccgacagcg accctgcgtg gccactaaag tctgcggctt
     4441 gcacgttgcg cagggaaaag ccctacccta tacaactata caataagtaa tattatttcg
     4501 tattatctct cttcctctat tgatattggt tttagtgtgc attgtgcaaa cctcacttta
     4561 aaaattaagc gcgtacagaa cgttaacgcc cttgcacaca tcaattgtct gcgcaacact
     4621 gtgttcgcat cgacgctcac aaaagcccca gaaccccctc gcggggctga attttgcagt
     4681 agaacggcct tcacgcctgg cacacacctt gcacacgaaa gatcaattct gctcttgggg
     4741 gcgaaccgcc gcagcagtct ctggagctgc gactagtgcg taatctgtgt tggcgggtcg
     4801 cactcgccga gcgtgcggat ctcgatgtat ttcgccaata gcgcgatact gtgtttccgt
     4861 aattgcgtgt cgctcttttc tgcgcagcgc cagcatactc cggtcagtat gatctggttg
     4921 acccgcgggt cttcggtgtg ggtaactaca aacgtgagcg gcggctgcgc agggtcgcaa
     4981 aaggcatgct cgcaggtgag acactgcggc gccttatcgg accccttccg gcattttgcc
     5041 cgccactcgc cgagtccatc gaagaactta ccggcgagtg aatctcccgc ctgctgtttc
     5101 tgccacaacc gttctaggtc gcgatcacgc cgcatgacga agcattccca gatcacctgt
     5161 tcctctattg gcatcttatt cccttgtttt actctgacga cgaattgagg ctctgcgtgc
     5221 gtatgagatc ggcgagcacg tcacgctcgg cgttgacaac ctgcgtcacc cgctcctctg
     5281 ccatatcaag ccgcctgatg atcagctccg tatcgactcc gcgccagcgt aactgatcag
     5341 cattcgtgcg ggcatagatg atctcttgcc gcttgcggta tttgatgcgg ttgttcggtt
     5401 cgcttcgctc ccgcagccgg tacgcgttct tggcgacggt gtgccgggcc ttgtccgacc
     5461 aatcgccggt cttgaagtca ccgaaatagg cgatcaacct ctgcatctgc gtcatcgtga
     5521 atagcggaca tccatcaagt gcgtctattg ccgtcccgta ggcatcctca agtcgtgtct
     5581 cgcccatccc gatcattttg agccgcccgg caaagggcgg cgggtcgccg tagggatcaa
     5641 acacgtcgag gggcactgcc ggccgcatga gcagcgctcg atgaagcgca ccgatgtttt
     5701 ccggcacccc catccaggct cggatatcgg ccctctcgat tagcgtcatc ttgctgccgc
     5761 aggcgatgac gcagaaacgc cgatcggtgg gtggcagctt cactacgtcg cgatggttgg
     5821 ttgcgatcat tatggttctc gccgagcgct gcgcgtaggc gtgctgtcct ttcgcctcga
     5881 agcggtgccg cgccgttggc gacggctcga ttgcattctt tagcgcctcg taattcagcc
     5941 gccgccgtgc ctggtgatgc ccgtcctcgt cggcggcttc gttgacggtg gcgatgagcg
     6001 cattggcgag ccggtcgttg aagcgcgctc ccgccgcggt cccggttatc tcgccgaatg
     6061 tgcatggaac gacgtagtcc tcgccaaata agcgttcgag aatctcgaac agtgttccgc
     6121 ggcctgttcc gaattcctcc gccaccatga tgactgcgat catcggcacc cacggcttgc
     6181 gggccttgtg ggcgaggtag ttccacatcc actcccgctc cgtgtcgtcc ggaatgagcc
     6241 gcgcgaagaa agctttgaag gtcgcgatct cacccccact tgtcggatgc gccggtggcc
     6301 agtagcgatt gtagatgtgc aagccctctt cctcgaacgt cggccgcggc cgatctgacc
     6361 gggtctgaat cttgtcgatg tgcacccgca gcgggtgctt catccagtca tcaacgacgg
     6421 atatcgtcct gctgcgccca cctcttggcc cctcgactgt aagcgcatag ggcagcatga
     6481 agccacgcag gcttgcaatc gatcctagct cgccatcgac caacgaaatg acgccgccct
     6541 tccccatgaa ggcgctcttg cagaaggcgt agttcaccag catccactcg agagcggcga
     6601 cgaagcacgc tctctgcgcc gcctccactg cggccgccga ggccgtcaaa gcaagcgcgg
     6661 cgtagtcagg cagcggcggc ggcggcacgc tgtagagcgg gttgcgcatc agcgggtggt
     6721 cgctgagctt cgccagaaat tcccggtcgg cccggctgat agcttcgcca tcgaaatcgt
     6781 cagggctcgt cttggaggca tactgttcgc tcattgaatg ggttcctttc cctcaactgc
     6841 tccaggaacg tgaacagctc gggcggcgcc cgctcgcgcc gatgccaggt tgtttccgtc
     6901 atggtgtcgt agatgccgag gccatggcgg ccccagttga tgagatgcga gtcggttcgc
     6961 acccgcgttc gatcgtgaaa gctgcccgag cagcgcatcg tcgccgcacc attgcgccgc
     7021 gcgcgcatat ggcactccag ctcggcgacc gtcatcacgc catggtcgcg cgtctcgatc
     7081 tcggtatcgg cgtcaagctc aaagcagcgc aattccgcct cgccccgccc gcccttgctg
     7141 acggcgacga gccctgccgc cgcagcgatc tcgtcgaaca agtcgcaggc cagcgcatag
     7201 gctgcttttg gcagcgccgg cagggctgtt cgcgacgtcg tcgccggcga ggcgccgccg
     7261 gcgaactggt acacgtccat aacctcgcct gcctggttgc gcgcgtgcgg cccgtcgacc
     7321 gcgaactgcc gcgtcccgag cgagccgaag cattcgactt gatgcttcgg tacggcgggg
     7381 tcgtcgggat cgtcgccgcg ataccagcgc cgcgacgcga gccgccggaa cggctcgtcg
     7441 acgcgcgcga tccaagcttc cttcggcccg ccggcatggc gtacgaggcc atgctcgaac
     7501 agcgccggga agttcttgcc gagtgcatcc gccagggccg cgacgagacc cgcgtccgcg
     7561 acatcgacat cgatcaccgc cagatcgccg tcgagcctca tgccggtcga tagcaacgcg
     7621 ctgcgatccc acgacagcac ctcggcttca tcgacggtac gcttcggcca gcccttctcg
     7681 atcggccgct tgcaatcggt cagcagcggt tgaaaaccat ttgccagcat ggccaggcgg
     7741 aaggcggtgc gctcctgtaa cggctttttc attttgctca ttcgcggttc acgatgacgt
     7801 cggcgttgcc gctcactttg cgcgcggcgg caagcaacag ctcggtatcg cgtagctgcc
     7861 gcttcagtgt gcgggtttgg cgcaggtcat aagctgcgaa ttcgttgatc ttgtccgacg
     7921 cgatgctgct gtatctttgc gcattgagca tttgccagta ggctaggtca tgcttctcgt
     7981 gctcgacgat ggcctgctga atttgctcca tgttcgccgc gcagcgcggc acttcgcgcg
     8041 ctaagtggtc ttgaaatcta ggccagcatt cggagccgat cgcacgtgca atcgatagcg
     8101 gatcgcttaa agctcggccg cagatggcgc actcgctgcc gttaccgatg tgcttgccaa
     8161 gcaggtagtc cttggtgatt cgagcaatct tccgctcgcg gcggcgtctt cgcgtggcga
     8221 ggctgcgcgc acgcgtttcg gcggtcattc gatccagctc ggtgcggaga tattcgcgac
     8281 atccgtcgat tgtcgggaat gccgtgctcc aaccgcgcga ccttgctccg ttgagcctcg
     8341 cgtaagtcgc gtagaaggca ttctcgctgc gccgaataga gccgcgataa tggccgcaat
     8401 cgaattgttc tgtgatcggc aggtcgaaga tacgatgcat cagatttgct ccagatgctg
     8461 tttaaatgcc tcttgtgcgc tcatcttgcc gagcacgcga ttgcgtttca tctcatcgac
     8521 cgtgcgcgtt gcgacgcaga cgtgaatggt cacgtgccgc gtctgcccgg gccggtagat
     8581 gcgcgcgatt gcctgttggg tgagttcggc agaccagctc ggcgacagcc atgccatgcg
     8641 cgagccgccg tattgcaggt tgatgccata gccggccgcg gcggggtgaa agtgaaaggc
     8701 gagcagtggc aacctgcccg cattccaggc ctcgaccagt aacgccgaat ccgcagcgct
     8761 ggtcgggccg ccgagcaccg gcacttccca ccaccacttc agcgcacgct ggatagtccg
     8821 caggtcctcg atgaactcat aagcgatgag cagcggctcg ccttcgaggc tctcaaccaa
     8881 ttccttcagc cattggattt tcaggtcgtg aacgaagacc gcatcctcgt tgcctgcatc
     8941 gtagaggaag ccgttggcca tctgcgccag cttgccggtc gcgatcagcg gcgaggcggc
     9001 ctcgatcgtg cgcccttcgg cggtcgtgaa cagctcccgc gccatctcgc gatagcgcgc
     9061 catcacctct gccggcagcg cgacgtgcgt ctccacgacg ttgagcggcg gcaggtcagg
     9121 catgtctgca tcggcgacgg tcatcgccac ggtgccgaac gcggcggcga tcttcgcttc
     9181 tgctcccggc agcgccaccc attcgcgtcc gaacggatcg cgcggccgga aatgccgctt
     9241 ctgccatggg atgaaggcgc ggccccatag cgccccgttg gtgatgatgg cggccggcat
     9301 gaacaggtcc atgctcgagt tgggacgagg agtccccgtc aggccccagc gcgtgcggaa
     9361 gcgcccgggt gaccattggg aaaagcagcc agttcctcga cgagccactg cgttaagtcg
     9421 ataccgatta ccgtcacctc gcgctcgggt gcagtagcga ggagcgcccg tcggcgctcg
     9481 ggcgtgccgt tgagaacagc aacgcgaagg tgctcgaggt gtggccacag cgtaacctcg
     9541 agcggccaca cggtcgtggc gacgagcttg ggtgcgatca caagcgcgtg gcggcgatgg
     9601 ccgtcacgga cgaggtcggc gagcgctgtg agcgcagcgg cccccttgcc agcgccaaga
     9661 ggtgccacca tgaatgcgcc atcatgctcg tagagccacg tggcggctcg ctgctgatag
     9721 ctgcgcagga cgcgcgcggc gaagggcgcg ttcatgacgc ttcacctttc aacgtagttt
     9781 tgctttttga gtatctacgg caggactgtt cttcactctg aagcggcaag ccatcagccc
     9841 agttcggtag ggtcagcatc tcgcgccgca ggatcgcctt cgcctcgtcg gcgcgcgccg
     9901 cactgatttc gcagacgatc tcatcgtgcg tcgtcatacg gattggcatg aacgcgagcg
     9961 ccggattggt ttcgattcgg gtaaccgtcg cgcgcaggat gtcggcagct gtgccgctga
    10021 ctgcgttctc gcacagcgtg ccatgccaga gtcttgcgcg cccgtgcgca cgccggaacg
    10081 ataattcggt gcgtttctcg ccggtcggct tgccatcttt acgcacgtcc acttcgcgcc
    10141 agcgcagtcg cggataggtc agcaagcggc ctgacggcag cgccatgaac agcgcgccgc
    10201 cgaggtagtc atcgcgatag atgaaagcga gccggcctgc cgtcgtgatc cggccgggta
    10261 tttcccaggc gctcatcgcg gcctcccaca gccctgacca gaactcttgt gcccatgggt
    10321 tagccgcgcg ccaggcgtcg acgatatggc gcgcctcggc ggtctcgaga tggatgcgat
    10381 agccgagtgc catcgccaag agcgccccaa cagcgccgcc gaagccgagc gccaacgtgg
    10441 caaccttgcc gatctggcgc tccgatttca ctatcgcgtg cgcgtctttg tgcaggatgt
    10501 cggcgatggc gacggtgtag atgtcaggcc gcgtcgggtc gcgatcgttg gcgcggaaaa
    10561 tctcgaggac gttctcggcg cctttcgaag cggcgagcca gggcgtgatc cgagcttcga
    10621 tcgctgacca atccgaccat acgaacaact ttcccagctc agcgatgagg gcaggtcgga
    10681 caataagggc gagcttgcgc gttgccggca tgtcgactgg atcggccgcg accaatgtcg
    10741 cgtaatcgca acccttggcg atcgcttcga ccagcgggct ttccgcggca ccgtcctcgc
    10801 cgagcacgtc gcgcgttaag ttctggattt gcgctccccg gctggtcatg cgcccggttt
    10861 gataggcacc ggcaaagcgg tattggcccc gcagcatgcc atcgagctgc tgcgctttga
    10921 gacgcgcaaa cttcttcggc gcggctccgg cgccatagac gcgcaaggtc gcggcctcat
    10981 gcgccttcac ctcgcttggg ctagggccgc cattggcgcg cttggcgtcg agcatggcga
    11041 gaacgcgttc gacccgatcg cgcgtcaggc taagctctaa ttcagcgtca tcgtcatcgt
    11101 cggaagtgcc aactatcagc acttcgcgca tcggcgcctc gacgagcatg tcgcacatcc
    11161 aggttgcgag ccgccccgcc tgcgtgactc gttcgacagc gccgccggtt agttcgacca
    11221 gccgacggtt gatggcgatg gcatcttggg ccgccagagt ggcggcgtgc tgcacgaacg
    11281 gcatatcgat cgccacgcct ctctggttga tatgttcgaa cgcccaatac tgtcgccatt
    11341 cttcggacgg cagcggtcgc gtccggccat agacttcgcg catcgcttca atgtcttgac
    11401 gcgcataggc gagaaagcgc tcccactccg ccggatattc gcgcgggctc gcaccttcga
    11461 tgcagaacat tcgaattagc gccttgccgt ccttctgttt gccggcgccg ccgagatagc
    11521 gagaagcgtg ttcgagatcg gttggcaggt tggaaacgcc ggcttgaatc atcggatcga
    11581 tgacgcgttc cggcgcaagc aaaggaaatc cgagcgtggc gtagttccag acggcagcgt
    11641 cgaagcttgc gttccacgcg gcaaaggttg cgttgcgttc gaaggctgcg cgcagctcgt
    11701 ccggcgcgtg gtcccaatcg aggatcgcac catcagcgtg ccaggtcagc gccggcgcgt
    11761 tgccgatcgc ataggccagc acgatggcgc gcgtcgaagc ttcagcgacg tagcgatatg
    11821 tgccggcggc ctttagatcg agcgcgccgc cgtagacctc gaaatccgtc caaatgagat
    11881 cgtcggcttc gaacatcgca gcacccggaa cctgaaaagt caggcgaggc tgctgcgcag
    11941 tcagcctcgc cttgtcaggt gcgcgcgtac gcgcaccgcc ttacgcgacg cgtcgccgcc
    12001 gcggttgatc ggtcggcgac gctgccgacc ccgacgtcgg tgccggtgtc gccggcgtcg
    12061 gagccggcgc tggaccgtca agcgacatcc agccgacagt gttcatcacg ggggtccaaa
    12121 ccctcccgta ctgaacgtgt tggtaggaat ccttttcgag cacctcgatc ggcacgatct
    12181 tgctgtcgtg ctggccgctg ttgagccggt cgcgtacctg atcgagcagt tggacgaggc
    12241 attggatggc gccaacggtc gtcgctttga agaccacttc gacgccggcg tcggcgccgt
    12301 cgagacactt catgttgacg gcccattctt cttgccacgg gaatcctgtg tcaggcattt
    12361 ccgcgaccag aggcttcggt tggtcgatag gaacaaggcg ttcgtcaacc acctggttgc
    12421 cgttgaagca gatacatccg tatttgaacg tcaacggatt gacggcccat cgactgcctt
    12481 cttcgggaat ggtgcgcttc tgcccaaatc cccaagtgcc gctgccttca cggctcttga
    12541 acaacatcat cggcagaccg gtacggccga ggatgactgt cgtgttgatt ttggcgagcg
    12601 ccgtttggag cgccgccaag gaggtgagcg cacctcccgc cgctggcgcg ggggcgactt
    12661 gcttgctccc gggcctttcg tgttcgccgt tgtcgttggc gtgatcgcgg tcgcgatcat
    12721 gatcatgatc gtcactcatg cttgcctccc tccttgaaag gctttgagcg cctcggaaaa
    12781 tgcgcgcgca gtctcgaccc gtccgggcgt cggggcgtgc acgttctcga cccgcacgag
    12841 cgagacgccc gagcgattcg aaacgatcaa ttcttgtggg attttgaggc cgcgtgcttt
    12901 cgctcgcagt tcgacctgtt tcggcgagtg cagcgtcttc gccacgacgt cgtcgcggtc
    12961 gaggccgagg ctctccagcg cagcaattgt agtactttca ttgttgagcc agcagcgcgc
    13021 ggcgcggccg gccgagagcg tgtatccggg cacgaggtcg ccgtcttcga gcgcgcgctt
    13081 cgcctgatcg cgcaacgccg tgtgaatatc tttgaccgca tcgacgaggt tcagcccgtc
    13141 agcgagtagc tgcaaatagg c
//
LOCUS       DATLJT010001555         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2216505,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001555 DATLJT010000000
VERSION     DATLJT010001555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216505"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VM910_35665"
     CDS             complement(<1..507)
                     /locus_tag="VM910_35665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI67880.1"
                     /translation="MDNIESLLHGFTIAVTLPHLGLMLIGVLLGILVGVLPGLGAPNG
                     VSLLLPLTFGMQPVSAIILLSSMYWGALFGGSVTSILFNIPGEPSSVATTFDGYPMAR
                     AGRATTALATAFGAAAFGALVGVVLITFLASWVAQVALAFGPPEYFAVYFLAFASFVG
                     MGGAPPIKT"
     gene            complement(518..985)
                     /locus_tag="VM910_35670"
     CDS             complement(518..985)
                     /locus_tag="VM910_35670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI67881.1"
                     /translation="MISRRALELATAALTGTFGAAVIVSSIDNGIGWSTAGVDAGTFP
                     FLTGMIILVGSLWNLARGWLQSQDVIVSSSDLRHLTALFVPAVVFVAAIPLIGVYLAS
                     ACYMLGTLVLLHRFSLLRSLLIATATPLALYLVFERMFQVSLPHGALGDVIGF"
     gene            complement(998..1999)
                     /locus_tag="VM910_35675"
     CDS             complement(998..1999)
                     /locus_tag="VM910_35675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67882.1"
                     /translation="MGSAAKLMMLSAGAIVVLFVAPAAAEWRPSKPIEFVATAGPGGG
                     TDNLARTVQSIIVKYKLTDQPVVVVNKGGGSGAEGYTYGKALAGDPYKVIFGTSNVWQ
                     QPMVSRVAFKHTDLTPIAAMAQDEFLLWVKQDAPYKTVGEYLKAVAAKPGEFKMGGAQ
                     SKDTDEVLTRMIEKAANVKFTYIPFKSGAEAAVQLAGGHIDSHVNNPNESLGQWRGGT
                     QRPLCAFSPQRLPKGPNITATEGWHDVPSCVESGLNIPKFQQPRTVWLPGKVTPDQVA
                     FYVELMKKVQATPEWKEYIERTSQTDTFLTGADFDKFIKEDIEHVRKIAAEQGWLVN"
     gene            complement(2023..>2914)
                     /locus_tag="VM910_35680"
     CDS             complement(2023..>2914)
                     /locus_tag="VM910_35680"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI67883.1"
                     /translation="LIYCSLSGFGRTGPYKHRRGFDLVAQAMSGIMSFTGESPHGPPV
                     KCGAPLSDITAGILAAMGILAAYTHRLKTGQGQWVETSLFEAALVQTYWQSAIALATG
                     VAPRAMGSAHPLNAPYQAFEASDGWIVVGGANQKNWLRMLDALNAPELADDPRFQSGA
                     DRMAHLKELEAELGARFRTKPAQYWLEALEEKGVPCGPVYDMLQALVDPQTLAREMVV
                     AVEHSRLGAVKTLGLPVKFSATPGKVCTGAPLYGEHTRQVLHEYGFDDQQIDSFEKEG
                     AIVTSMPRRARERWPDAVTP"
BASE COUNT          567 a          892 c          905 g          550 t
ORIGIN      
        1 cgtcttgatc ggcggagcgc cgcccattcc gacaaagctc gcgaacgcga gaaaatagac
       61 cgcgaaatat tccggggggc caaacgccag cgcgacctga gcgacccaag aggcgagaaa
      121 agtgatcagc acgacgccga caagcgcgcc gaaggccgcg gccccaaacg cggtcgctag
      181 cgccgtggtc gctcggccag cgcgcgccat cggatagccg tcgaaggtgg tcgcaaccga
      241 tgacggttct cccggaatat tgaagagaat cgacgtgacg gacccgccga acagcgcgcc
      301 ccagtacatg ctggacagaa ggatgatcgc ggacaccggc tgcatgccga acgtcagggg
      361 caggagcagc gaaactccgt tgggggcacc cagccccggc agcacgccga ccaggatccc
      421 gagcagtaca ccgatcagca tcaacccgag atgaggaagc gtgactgcga tggtgaaccc
      481 gtgcagcagg gattcgatgt tgtccatcgc gtcctcgtca gaatccgatc acgtctccca
      541 gtgcgccatg cggcagcgac acctggaaca tgcgttcgaa taccagatag agcgcgagcg
      601 gggtcgccgt cgcgatgagg agtgatcgca gcaaagagaa ccggtgcagc aggaccagag
      661 ttccgagcat gtaacaggcc gaggcgagat acacgccgat cagcgggatc gcagcaacga
      721 acacaacggc cggaacgaat aacgctgtta ggtgccgcag gtcggacgaa ctgacgatga
      781 catcctggct ttgcagccag ccgcgggcaa gattccacag actgccgacg aggatgatca
      841 tgccggttag gaaggggaat gtcccggcat caactcctgc ggtcgaccag ccgataccgt
      901 tatcgatgct ggagacgata acggccgcgc cgaaggtccc agtcagagcc gcggttgcca
      961 gttccagagc gcgtcgcgag atcatcgacg ccgcggctca attgacgagc catccctgct
     1021 cggcggcgat cttgcgcacg tgctcgatgt cctcctttat gaacttgtca aagtccgctc
     1081 cggtcaaaaa agtgtcggtc tgtgacgtgc gctcgatgta ttccttccac tcgggcgtgg
     1141 cttgcacctt cttcatcagt tcgacgtaga aggccacctg atccggcgtg accttgccgg
     1201 ggagccagac cgtgcgcggc tgctggaact tcggaatgtt caggccggat tcgacgcagc
     1261 tgggaacgtc gtgccagcct tccgttgcgg tgatgtttgg gcctttcggc agtcgttgcg
     1321 ggctgaaggc gcacaacggg cgttgcgtgc caccgcgcca ctgcccaagg ctttcgtttg
     1381 gattattgac gtgcgagtca atatgaccac cggcgagctg cacggccgct tcagcaccgc
     1441 tcttgaacgg aatgtaggtg aatttcacgt ttgcggcctt ttcgatcatg cgggtgagaa
     1501 cttcgtcggt atccttcgat tgcgcgccgc ccatcttgaa ttctccaggc ttcgctgcca
     1561 cggcttttag gtactcgcca acagtcttgt acggtgcgtc ctgcttcacc cagagcaaga
     1621 attcgtcttg cgccatcgcc gcgattgggg tgaggtcggt gtgcttgaag gcaactctgg
     1681 ataccatcgg ctgttgccag acattcgatg tgccgaagat caccttgtag gggtctcccg
     1741 cgagcgcctt gccgtaggtg tacccttcgg ccccgctgcc gcctcctttg ttgacgacca
     1801 cgacgggctg atcggtcagt ttgtatttga cgatgatgct ctgaaccgtc cgagcgagat
     1861 tgtcagtgcc gccgccgggg ccggcggtag caacgaactc aattggcttg ctcggccgcc
     1921 attcggccgc tgcaggagcg acaaacagca cgacaatggc gccggcggat agcatcatca
     1981 attttgcagc ggagcccatg tttcctcctc ttcttcttta gcttatgggg tgaccgcatc
     2041 cggccaccgt tcgcgggcac gtcgaggcat cgacgtgacg atcgcgcctt ccttctcgaa
     2101 actgtcgatc tgctggtcgt cgaatccgta ttcgtgcagc acttggcgtg tatgctcgcc
     2161 gtagagcggc gcgccggtgc acaccttgcc aggtgtggcc gagaacttca cgggaagccc
     2221 gagcgtcttg acggcaccga gtcgcgaatg ctcgaccgcg accaccattt cgcgtgcgag
     2281 cgtctgcgga tcgacgagcg cttgcagcat gtcgtaaacg ggaccacaag gcacgccctt
     2341 ttcttcgagc gcctcgagcc agtattgcgc cggcttggta cggaatcgcg cgcccagttc
     2401 cgcctcgagc tctttaaggt gagccatgcg gtcggcgccg gactggaaac gcggatcgtc
     2461 cgcgagctcg ggtgcgttca acgcatcgag catgcgtagc caattctttt ggttggcacc
     2521 gccaacgacg atccatccgt ccgacgcttc gaatgcttga tagggggcgt ttagcggatg
     2581 tgccgagccc atcgcgcgtg gagcgacgcc ggtcgcaagc gcaatcgccg attgccaata
     2641 ggtctgcacc agtgcggcct cgaacagcga ggtttccacc cactggccct gcccggtctt
     2701 cagtcgatgc gtataggcgg ccaggatgcc catcgccgcg agaatgcccg cggttatatc
     2761 cgacaggggc gcgccgcatt tcacgggcgg cccgtgcggg ctctcgccgg tgaagctcat
     2821 gatcccgctc atcgcctgcg caacgagatc gaagccgcgc cgatgcttgt agggaccggt
     2881 gcggccgaac ccggagagcg agcagtagat caac
//
LOCUS       DATLJT010001556         2028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_377713,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001556 DATLJT010000000
VERSION     DATLJT010001556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377713"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..949)
                     /locus_tag="VM910_35685"
     CDS             complement(83..949)
                     /locus_tag="VM910_35685"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HVI67884.1"
                     /translation="MAHRPFASAPYVVAKSALAPAEPHIPAEESLCPIVSHDWVNSEY
                     KHLVVDASPKALAVKPGQFFNLLCPSPDAGELWLRRPQSVYRIDPQNRRVEFLYKCVG
                     RGTYGLATLEAGDELNMVGPLGVGFTIEPACKHIVVLGRGVGLATLAPISQLAGERGI
                     GVTAILSARSAEFVMADDLFGRVGEVIAVLDTDGTSAVEHVEAILERLIADRRADAFY
                     TCGSNRLLQLMKRVGKRRGIPGQVAMEQVMACGLGPCYVCVRTFEVDGKKELRRVCIE
                     GPVFDLQEAVGW"
     gene            complement(1071..>2028)
                     /locus_tag="VM910_35690"
     CDS             complement(1071..>2028)
                     /locus_tag="VM910_35690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVI67885.1"
                     /translation="STRVRPCHAFCHGQARARRRQNVQPVMAIRFDDIGNRLKAFRLG
                     SGLSADEIANRLGISRTALYRFEKGELAKIETLEKLAELLSVSVPTLLGVGVEYVASA
                     VSYFERMRQIEESCEHIIVLAGPISYLLASDAFDQALGDILRESIPDALPGRKRALDQ
                     VDEIIAVLRQRKDTYRRRQPGIVNLISAIEMEHFLQNGLVGRLGLPEKVRRERRALAR
                     AEAEHFVTLMQDEPIGVQIGIVPDTLPHASFQLFRQPDRQVLALSPFRLGEQPNIRVG
                     VAMITSAPEALALHEKMAKDLWQRALKGTAAVRLMRSLLERP"
BASE COUNT          369 a          661 c          651 g          347 t
ORIGIN      
        1 tgcgcaagat cggtcgagaa gcatcccgac gccggcatga tcgggttctt gagcgccacg
       61 ttcccgatct tcacgcgcat gtctaccatc cgactgcctc ctggagatcg aataccggcc
      121 cttcgatgca cacgcggcga agctctttct tgccgtcgac ctcgaaggtg cgcacgcaga
      181 cgtaacaggg gccgagccca caggccatga cctgctccat cgccacttgg ccgggaatgc
      241 cgcgcctctt gccgacgcgc ttcatcaatt gaagcagccg gtttgagccg caggtgtaga
      301 acgcgtcggc ccttcggtcc gcgatcagcc gctcgaggat cgcctcgacg tgttcgaccg
      361 cgctggtgcc gtcggtatcg agcacggcga tgacctcgcc gacccgcccg aacaggtcgt
      421 cggccatcac gaactcggcg ctgcgcgcgc tcaggatcgc ggtgacgccg atgccgcgct
      481 cgccggcgag ctgggagatc ggcgccaggg tggcaagccc gacgccgcgg ccgagcacga
      541 cgatgtgctt gcaagcgggc tcgatggtga agccgacccc gagcggcccg accatgttga
      601 gctcatcgcc tgcttccagc gtcgccagcc cataggtgcc gcggccgacg catttgtaga
      661 gaaactcgac gcgacggttc tgcgggtcga tgcgataaac gctctgcggg cggcgcagcc
      721 aaagctcgcc cgcgtccggc gacgggcaca gcagattgaa gaactggccc ggcttgaccg
      781 cgagcgcctt gggggaagca tcgacgacca ggtgcttgta ttcggaattg acccagtcat
      841 gggagacgat cgggcataag gactcctcgg ccggaatgtg cggctcggca ggcgcgagcg
      901 cgctcttggc cacgacgtag ggcgccgatg cgaacggccg atgcgccata gggaaaagcc
      961 cgtctggaaa acgcctttgc tgcccagccg gtacccgcga tctgtatcaa aatcaagatt
     1021 ttttccaaat tctgaacaag atggtcggaa gcctacctac catcctacct ctagggccgc
     1081 tcgagcagcg accgcatcag gcggaccgcc gccgttccct tgagcgcgcg ctgccagaga
     1141 tccttggcca tcttttcgtg cagggccaat gcttcgggag ccgaggtgat catcgccacg
     1201 ccgacccgga tattgggctg ctcaccgagc cggaacggac tgagtgccag cacctgccgg
     1261 tccggctggc gaaaaagctg aaagctcgcg tgcggcagcg tgtccggcac gatgccgatc
     1321 tgtaccccga tcggctcgtc ctgcatgagt gtgacgaaat gctcggcctc cgcccgcgcc
     1381 aacgcgcgcc gttcgcgccg caccttctcc ggcagaccga ggcgaccgac cagaccgttt
     1441 tgcaagaagt gctccatctc gatcgccgag atgaggttga cgatgccggg ctgacgccgg
     1501 cggtaggtgt ccttgcgctg gcgcaacacc gcgatgatct cgtcgacctg atcgagcgcg
     1561 cgcttgcgtc cgggcagggc gtccgggatg ctctcccgca agatgtcgcc gagggcctga
     1621 tcgaaggcgt cggaggccaa gaggtaggag atcgggccgg cgagcacaat gatgtgctcg
     1681 cagctctcct cgatctggcg catgcgttcg aaatagctga ccgcggaggc gacgtattcc
     1741 acgccgacgc ccagcaacgt cggcaccgac accgacagga gctccgcgag tttttccagc
     1801 gtctcgatct tcgcgagctc acccttctcg aagcgataga gcgccgtgcg cgaaatgccg
     1861 agccggttgg cgatctcgtc ggcgctcagc ccggagccta ggcgaaaagc tttgaggcgg
     1921 ttgccgatat cgtcgaagcg gatggccatg accggttgaa cattttgccg ccgtcgtgcg
     1981 cgggcttgtc catggcaaaa tgcgtggcat ggccgaacac gcgtcgac
//
LOCUS       DATLJT010001557         6232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_147235,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001557 DATLJT010000000
VERSION     DATLJT010001557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147235"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VM910_35695"
     CDS             <1..268
                     /locus_tag="VM910_35695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HVI67886.1"
                     /translation="PLVLHCYAGISRSTAGAYVAACALNPRRDEWVIARELRRASATA
                     TPNSRLVALADRMLGRDGRMVAAIDAIGRGEIAYEADPFRLELE"
     gene            713..1144
                     /locus_tag="VM910_35700"
     CDS             713..1144
                     /locus_tag="VM910_35700"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI67887.1"
                     /translation="MGASGRTLQSALALIVMLGAGALVPAISQQQTPATKVTTLLKEA
                     IAELPGREIIMVTLDIPPGGGSPPHRHPGHHIFGYVLEGTYKLKLDQGTETILTKGQT
                     FHEAPGQLHAVSANASLTEPAKVLAFIVAESGKPVTVPEKQ"
     gene            1379..2380
                     /locus_tag="VM910_35705"
     CDS             1379..2380
                     /locus_tag="VM910_35705"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67888.1"
                     /translation="MGQNSYSRGTTRRAFVVSLGPGMYVLGMAVANGALLKKVPTATG
                     IDALYVPFVVAAELKIFEKYGLETSYKPFDDGNVALDALLAGSSDIGATTELGGLSRW
                     DKGGKLYVTSYSSTSGQQIGLAGRDDIKRPEDLIGRTVAYPRASGGHLYFMRYVKKYN
                     LPVDKIKVKFMQAPEMVAALERRDIDAFFLWEPWLDKAAQLVQGARVLARSGDDGVFI
                     LTSYDYYSQGLIDDHPRAAAATRALIEATDFCNTNAVEAAKVAGKAFRIPEGDMKVYM
                     SRMKYRVEMPKDVVLGNFRGAAEVALGEGIIKKMPDWNDFVRPQLMKEVAPDRAVGW"
     gene            2430..3209
                     /locus_tag="VM910_35710"
     CDS             2430..3209
                     /locus_tag="VM910_35710"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67889.1"
                     /translation="MAEEFLVARDVSYRYAARRGVEAVPVLQRFSVSLTEGEFFCLLG
                     PSGCGKTTVLNLMAGFLEPVSGMLTLAGRPIRGPGIDRTVVFQGDDALFNWLRTIDNV
                     AFGLRMQGMSKERRHAVARDYLHLVHLDGQDNKYPGELSGGMKQRVQIARVLASDPKV
                     LLMDEPFGALDAQTRGRMQSELVEIWHKTGKTIVFITHDITEAVLLADRIGVMTKGPE
                     SRLDAIIPVDLPRPRRRSSPGFGEMWERINALIENEEANGP"
     gene            3206..4036
                     /locus_tag="VM910_35715"
     CDS             3206..4036
                     /locus_tag="VM910_35715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67890.1"
                     /translation="MTEVSPVRDARPADAVAPAPRLPLGGIKRDHLIYITSIIGLIML
                     WHLIATTFFKPQFFPPPLLVLSTGREMLESGELMEHVSISLQRILAGFLIGSAIAAPA
                     GLLMGSIPIVRAIFDPYVQFFRFVPSLAWLTPVVIWFGIGETSKVLIIVYTTIFIVLI
                     NTMVGVSHITPNKLRAAACLGATPLKAFIFVTLPATLPFILTGMRLAMGNSFATVVAA
                     EMIAADSGLGYLIFNSRLWMATDKIFIGIVCLGALGLITDRFFRYLIVRFAHQYGPIE
                     "
     gene            complement(4047..4826)
                     /locus_tag="VM910_35720"
     CDS             complement(4047..4826)
                     /locus_tag="VM910_35720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI67891.1"
                     /translation="MMEWRNQVAVVTGGGRGLGRATARLLAQRGAAVCVNYAAHADAA
                     AAVVAEIAAAGGRAIAVAADVADEAGVEAMIARTEAELGPVTILVNNAGVAWQGTLDT
                     YDREQVARMRQVNVDGVIHATRAVMGSMRARRYGRVVNVASIAAIGTALSGNAFYAAT
                     KAEVVMLTRRFALELGQHGITVNAVAPGFVRTDMTQRGRGAADWPGTEERFAARAMMG
                     RIGEPEDIANAVVFLASPESGWITAQVLTVDGGRMDYIGHG"
     gene            5070..6068
                     /locus_tag="VM910_35725"
     CDS             5070..6068
                     /locus_tag="VM910_35725"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI67892.1"
                     /translation="MEMRVYGRTGMQLSILGFGCGAVGGLMVRGDPLEQERTIARAIA
                     AGVNYFDTAVQYGDGESEKNLGRILQRLKPANVVVGTKVRLPSGDFGGIADAVAKSLE
                     GSLARLRLDRVDIFHLHNAITETGGGESLSVRQVLGDVVPAFERLRQQGKTRFLGLTA
                     IGETAALHQVIDARAFDSAQVVYNMLNPSAAVALPANYPAQDYERLFDHTGAAGVGVV
                     GIRALAGGALSGSTERHPIASPPPEPIGTAMSYDADVVRARRLMPLVKEGFAASLTEA
                     AIRFALSHPAMGTILVGMASPQQFEDALAAVENGPLPPAAFDRLTALRQAFAGEPR"
BASE COUNT         1135 a         1995 c         1959 g         1143 t
ORIGIN      
        1 gccgctggtg ctgcattgct atgccggcat cagccgctcg accgcgggcg cctatgtcgc
       61 ggcctgcgcg ctcaacccgc gtcgcgacga atgggtgatc gcgcgcgagc ttcgccgcgc
      121 ctcggcgacg gcgacgccca acagtcgcct cgtggcgctt gccgaccgca tgctcggccg
      181 cgacggccgc atggtggcgg cgatcgatgc gatcggacgc ggcgagatcg cctatgaggc
      241 cgaccccttc cggctcgaac tggaatagcg cgtctcgccg ctctcctcct gtaaataaca
      301 ggagagaaac aggacgcccg gcacaacctg gcgcctgtga cctccccgta agctccagga
      361 agcaaacagg aaacgcgcgc atcccggcgc cagttgaggc ggcccggcgt gccggcaaga
      421 gcaggacgga acaggacgaa aacaggcaat tgatgcaact tggccttgcg gctcgtgcgc
      481 gccttgggcg tccttcgccg tcattgcacg tttgctcgta cattctaagc atggcgaatc
      541 tatacatcaa cttcatgtcg tagtcaacac acaaatacga aatgtagcct agatcgaaac
      601 cctcatctga aggcgcgcga tgcaattgac ggcatgttcc cgtgcgcttg gagcgaccgg
      661 acctttctga cattctcgct gagagatcgc aaaacttgag ttggaggcaa ccatgggcgc
      721 atcaggccgc acgctccagt ccgcgctagc gctgatcgtg atgctgggcg cgggcgcact
      781 tgtgcccgcc atctcgcagc aacaaacgcc cgccaccaaa gtcacgacgc tgttgaagga
      841 ggcgatcgcg gaacttccgg gccgcgaaat catcatggtc acgctcgaca tcccgcccgg
      901 cggcggctcg ccaccgcatc gccatcccgg gcaccacatt ttcggctacg tgctggaggg
      961 gacgtacaag ctcaagctcg atcagggcac cgaaaccatc ttgaccaagg gccagacctt
     1021 ccacgaagcg ccgggccaat tgcacgccgt ttcagccaat gcaagcctga ccgagcccgc
     1081 caaggttctc gccttcatcg tggccgagag cggcaagccg gtcaccgtgc cggagaagca
     1141 gtagcaagcg cagtgcagcg gtctcctatc acagcgtcta tgtgctatcc ttgaaagcac
     1201 aggaggcggc aaggagcccg cctcgaggca gctcgggtca gtggcagcgc tgatagggca
     1261 caggggagga agttcccccg tcccgacctt aagcggcgtg agtgtcggcg cgcttggcat
     1321 cgtcgtggcg atggcgagca ttcggtcaat tcaccggtgc aatcggaagg agaaatatat
     1381 gggccaaaac agctattcgc gtggaacgac gcggcgggcg ttcgttgtgt cgttggggcc
     1441 aggcatgtac gtgctgggaa tggctgtcgc gaacggcgcc ctgctgaaaa aagtgccaac
     1501 cgcaacgggg atcgacgcgc tctacgtccc attcgtggtc gcggccgagc tcaagatctt
     1561 tgaaaagtac gggctggaga ccagctacaa gcctttcgac gatggcaacg tcgccctcga
     1621 cgcgcttctc gccggcagtt cggatatcgg tgccaccacc gaactcggcg ggctctcgcg
     1681 ctgggacaag ggtggcaagc tctacgtgac ctcctactcg tccaccagcg ggcagcagat
     1741 cggccttgct ggacgcgacg acatcaagag gccggaagac ctgatcggca ggaccgtcgc
     1801 ctatccacgc gccagcggcg ggcatctcta cttcatgcgc tatgtgaaga aatacaatct
     1861 gccggtggac aagatcaaag tgaagttcat gcaggcgccg gagatggtgg ccgcgctcga
     1921 acgccgcgat atcgacgctt tctttctgtg ggagccgtgg ctcgacaagg cggcgcagct
     1981 cgtgcaaggg gcgcgcgtgt tggcgcgatc gggcgatgac ggcgttttca ttctcacgtc
     2041 ctacgactac tactctcagg gactgatcga cgatcacccc cgtgccgccg ccgcgacgcg
     2101 cgcgctcatc gaagcgacgg atttctgcaa taccaacgcg gtcgaggcgg ccaaggtcgc
     2161 cggcaaggcg ttccgcattc cggaaggcga catgaaggtc tacatgagcc ggatgaagta
     2221 ccgcgtcgag atgcccaagg acgtggtgct gggcaatttc cgtggcgctg ccgaggtcgc
     2281 gctgggcgag ggcatcatca agaagatgcc ggactggaac gacttcgtgc gtccgcaact
     2341 catgaaggag gttgcgccgg atcgggccgt cggctggtag gcctttcgcg gccggcggcg
     2401 gcccgcaggc agtgggagcg cggcggtcag tggcagaaga attccttgtg gcgcgcgacg
     2461 tcagctaccg gtacgcggct cgccggggcg tggaagcggt accggtgttg cagcgttttt
     2521 ccgtgagcct cacggaaggc gagtttttct gtctgctcgg gccaagcggc tgcgggaaaa
     2581 caaccgtgct caacctgatg gcgggcttcc tggaaccggt cagcggcatg ctcaccctcg
     2641 ccggccggcc gatccgggga ccgggaatcg atcgcacggt ggtgtttcag ggcgatgatg
     2701 cgcttttcaa ctggctgcgc accatcgaca acgtggcatt tggcctgcgg atgcagggca
     2761 tgtccaaaga gcgccggcat gccgtcgcac gcgattacct tcatctcgtg catctcgatg
     2821 gccaggacaa caagtatccc ggcgaactgt ctgggggcat gaagcagcgg gtgcagatcg
     2881 cccgcgtgct tgcgagcgat cccaaggtgc tgctgatgga cgagcctttc ggcgcgctcg
     2941 atgctcagac ccggggacgt atgcagagcg agcttgtgga gatctggcac aagaccggca
     3001 aaacgatcgt gttcattacc cacgatatca ccgaggcagt cctgctggcg gatcgcatcg
     3061 gtgtcatgac caaggggccg gagtcgcgcc ttgatgcaat tattccagtc gacctgccgc
     3121 gaccgcgccg gcgcagcagt cccggcttcg gcgagatgtg ggaacgcatc aacgcgctga
     3181 tcgagaacga agaggcgaat ggaccatgac ggaggtcagc ccggtaagag acgccaggcc
     3241 cgctgacgct gtggcgccgg cgccgcgcct gccgctcggc ggcatcaagc gcgatcatct
     3301 catttatatc acctccatca tcgggttgat catgctgtgg cacttgatcg ccacaacgtt
     3361 cttcaaaccg cagttctttc cgccgccgct tctggtgctg tcgaccggac gggagatgct
     3421 ggaaagcggc gaactgatgg agcacgtcag catcagcctg caacgcatcc tcgcgggatt
     3481 cctgatcggc agcgcgatcg ccgctccggc tggtttgctg atgggctcaa ttcccatcgt
     3541 ccgcgcgatc ttcgacccct atgtgcagtt ctttcgtttc gtgccttcgc tggcatggct
     3601 gaccccggtg gtcatctggt tcggcatcgg ggagacatcg aaagttctca tcatcgtcta
     3661 cacgacgatc ttcatcgtgc tgatcaacac catggtcggc gtgtcgcata tcaccccgaa
     3721 caagctgcgc gccgcggcct gcctgggcgc cactcccctc aaggcattca tttttgtgac
     3781 gctgccggcg acgctgccgt tcatcctgac cggcatgcgc cttgccatgg gcaattcgtt
     3841 cgcaaccgtg gtcgccgccg agatgatcgc ggcggattcc ggccttggct atctgatatt
     3901 caattcgcgc ctgtggatgg caacggacaa gatcttcatc ggcatcgtct gcctcggggc
     3961 gcttggcctc atcacggacc gcttcttccg ctatctcatc gtccgcttcg cccatcaata
     4021 tgggccgatc gaatagccgt tcggctctaa ccgtgtccaa tgtagtccat ccgcccaccg
     4081 tcgaccgtca gcacctgcgc cgtaatccaa cctgactccg gcgacgccag gaacaccacc
     4141 gcattggcga tgtcctcagg ttcgccgatg cggcccatca tcgctcgtgc ggcgaagcgt
     4201 tcctccgttc ccggccagtc cgctgcgccg cgcccgcgct gcgtcatgtc ggtgcggacg
     4261 aagccgggcg ccacggcgtt cacggtgatg ccgtgctgtc ccagttccag ggcaaagcgc
     4321 cgcgtgagca tcaccacctc ggccttcgtt gccgcgtaga atgcattgcc cgacaacgcg
     4381 gtgccgatcg ccgcgattga ggccacattg acgacccgcc cgtagcgccg cgcccgcatg
     4441 cttcccatca ctgcgcgcgt ggcatggatc acgccgtcca cgttgacttg gcgcatgcgc
     4501 gcgacctgct cgcggtcgta agtgtcgagc gtcccctgcc aagccacgcc ggcgttgttc
     4561 accaggatgg tcaccggacc cagttccgct tcggtgcgcg cgatcatcgc ctcgaccccg
     4621 gcctcatcgg cgacgtccgc cgccaccgcg atcgcccgtc cgcccgcggc cgcaatctcc
     4681 gcgaccacgg ctgcggcggc atcggcatgc gcagcatagt tcacgcatac cgcggcgccg
     4741 cgttgcgcca ggagccgcgc cgttgctcgg ccgaggccgc ggccgccgcc cgtcacgacc
     4801 gccacttggt tgcgccattc catcatgttg ccttttcata ggccggatca gcatgccggc
     4861 tagtgtcccg attccgaagt ttgtaaacct ttgcggcagc ctctgatgcg aacttcggaa
     4921 tcaaagggac actagcaact ctaggattct agtgcccctt ttgaacccga agttcgcaac
     4981 tgcccttgca gcacgacgtg tgcgaacttc gggttcggga caccagtatt ctatcgcgat
     5041 atatggctca cgcaggcgga ggaacggtga tggaaatgcg ggtatacgga cgcacaggaa
     5101 tgcagctctc gatactaggc ttcggctgcg gcgcggtcgg cgggctgatg gtgcgcggcg
     5161 atccactcga acaggagcgt accatcgcgc gggccatcgc cgccggcgtg aattacttcg
     5221 acaccgcggt gcagtacggc gacggcgaat cggaaaagaa tctcggccgc atcctgcaaa
     5281 ggctcaaacc ggccaacgtg gtcgtcggca ccaaggtccg gctgccgagc ggggacttcg
     5341 gaggcattgc cgacgccgtg gcgaagtcgc tcgaaggcag tctggcgcga ctacgcctcg
     5401 atcgggtcga catcttccac ctgcacaatg cgatcaccga gaccggtggc ggcgaatcgc
     5461 tcagcgtccg acaggtgctc ggtgatgtag tgccggcgtt tgagcgactg cgccagcagg
     5521 gcaagacgcg gtttcttggg ctcaccgcaa tcggcgagac agcggcgctg caccaggtga
     5581 tcgacgcacg tgccttcgac agcgctcagg tggtctacaa tatgctcaat ccgtccgcgg
     5641 ccgttgcgct gccggcgaat tatccggcac aggattatga acggctgttc gatcataccg
     5701 gggcggccgg ggtcggcgtc gtcggcatcc gcgcgcttgc cggaggcgcg ctctccggat
     5761 cgaccgagcg acatccgatt gcgtcaccgc cgccggagcc gatcggcacg gccatgagct
     5821 acgacgccga tgtggtgcgc gcccgccgcc tgatgccgct ggtcaaggag gggtttgctg
     5881 cgagcctgac cgaagccgcc atccggtttg cgctctcgca tccggcgatg ggcaccatcc
     5941 tggtcggcat ggcgtcgcca caacagttcg aggatgcgct cgccgcggtc gaaaacggcc
     6001 cgttgccgcc cgccgcgttc gaccggctga cggcgctgcg gcaggcattc gccggcgaac
     6061 cacggtgatc gctgagattg ccgagggcga aacggcatcg acgtggcgat ttccgctctt
     6121 gccgtgcgca ataaaaagac gtttctcccg accgatgcca gtagcggatg gcggtaggaa
     6181 gtgcttattt attccaccat gagcgtgccg ttatccagaa tggtggccct cg
//
LOCUS       DATLJT010001558         5391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1066853,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001558 DATLJT010000000
VERSION     DATLJT010001558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066853"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VM910_35730"
     CDS             complement(<1..183)
                     /locus_tag="VM910_35730"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HVI67893.1"
                     /translation="MRFQQGQSGNPSGRPRGALNRATVLAQELLSARVESIAGKLIEL
                     AESGDMRAIRVCMERLM"
     gene            complement(564..1538)
                     /locus_tag="VM910_35735"
     CDS             complement(564..1538)
                     /locus_tag="VM910_35735"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI67894.1"
                     /translation="MNKPVDSWQGRPITVECSGVTVTRLGASLGAEVSGVELRQPLSD
                     EAFNVIQNALIEHELVIFRNQEITSENLIDFGRRFGELTVHPFAPHEEKAPVLIKFRN
                     DENNPPFATDVWHSDETFRAEPPMATILCAKVVPAIGGDTMFASMSAAFDGLSGRMQQ
                     FISGLEAIHDWKPFKRLFDNTEEDRKNRQHFELLYPPQLHPVVRVHPVSGRNILFVNP
                     QFTIAIKDMDERESRSLLDTLFHQALIPEYQFRLHWAPHTIAIWDNRSTQHYAVNDYY
                     PQRRFMERITIRGGPVIGVKTADPHSVRKAKRIVSPDIERYGGHRPHR"
     gene            complement(1663..2685)
                     /locus_tag="VM910_35740"
     CDS             complement(1663..2685)
                     /locus_tag="VM910_35740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67895.1"
                     /translation="MRGTLQVGRSPALHAAGWLLKAMVAAAIGVFGYALTKSAQSFAG
                     SWPEHTVRIITPFSPGISVDVAARALADALAKRWKQPVVVENRPGADTIIGTQAFLEM
                     RDGHALLFTTHSTFTVVPLLRARVPYDPIGDVKPISLAVEDFLSVVVTPRLEIGSLAE
                     FVKLAREQPTKLNFYAVPGAPYLAYLAFQQRVGMTTTFVAYNNPVSAISDLSEGRIHI
                     AVMPLALVLGAVQADKIKLLAVTNAVRTPAAPNVPTVGEAGYPEFTFGGFLGLFGPRD
                     MPADLQERIATDARLILNEPEIQQRLTNVGLIARGTTPAEFATVMDGQRAKWSAIARE
                     HKIEPQ"
     gene            2861..4579
                     /locus_tag="VM910_35745"
     CDS             2861..4579
                     /locus_tag="VM910_35745"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HVI67896.1"
                     /translation="MDTAVHRAFRFAGYTLDMTRGTLRNGEHAIELRPKSFEVLRYLV
                     ENAGRLISKDEIIQAVWSNVAVSDESLTRCISDVRAALADEHQRVIKTVPRRGYIFDV
                     PVLETGMPGAAAAGVGSPETSAPAEQASGRARKSWPWLAAAAALVLVLAVVAAAVWLW
                     RQPAGLPLPDRPSIAVLPFINMGMDPRQDYFSDGITEDIITNLSKFSDLFVIAHNSTF
                     AYKGKSIEPRQIGRELGVHYLLQGSVRRQPDQLRITAQLIDATNSRQIWAEHYDRVPA
                     DIFAIQDEVTQKLIVTLVARISKSEFDRVLRKPPQTFAAYDYYLRGNAMVEEAFRGKR
                     GENIAAARAFFEQAIASDPRYAAAVQALARTYLLAWLEPTAYDPIAREYRQQATLDRA
                     LSLAQEAVALDGNLPEAHTALAWVLHWHYRRAESMAEYQRAFELNPNLAERSFAPALY
                     HNGRTREAIDYMRRIMRLDPFHPTQLFVNLGAAFYLSGSYAEALGPLRTATDRMPDFR
                     PGFVWRAAAAAQLGLDEEARRAASGVLHLQPDFKISEWLRFLRLAKQEDADRLADGLR
                     KAGLPE"
     gene            complement(4980..>5391)
                     /locus_tag="VM910_35750"
     CDS             complement(4980..>5391)
                     /locus_tag="VM910_35750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67897.1"
                     /translation="QAVNDLAEDRLQVYSASYAILRPQREAGRITVLAQMGRERAPSL
                     PDIPTGIEAGFPELEMDGNVGLFGSKALSAELRERIGADVVAVSNDKAIDDKLNATAQ
                     TPSRGGAKEFAASIERQRAQIATIAKALGIQPTR"
BASE COUNT         1004 a         1674 c         1668 g         1045 t
ORIGIN      
        1 cataagccgc tccatgcaca cgcgaatggc ccgcatgtcg ccgctctccg cgagctcgat
       61 cagtttgccg gcgatgctct cgacgcgtgc cgacaacaat tcctgcgcca gcacggttgc
      121 ccgattgagc gctccgcgcg ggcgtccgga cggattcccg ctttggcctt gttgaaagcg
      181 catagctgcc ttctcctgta ttttcctgtt tttttttgca aaaaacgaga aacccgccgc
      241 gcggccgcag gcgcggtcgc gcaacaaaaa acccggcgcg gcatgccgcc cgggcttctg
      301 gcgtagtttc ggtgaatacg cttttctcga agattctcgt tacacgagtc aagcggaaaa
      361 tggagttccc gttaagtccc ctgagttagt acacggattg tcggccatgc cctgagaagc
      421 gggcaaaagc ggcggggatc tcgtacgtac gctatgtgcc aaaaggcagc cttacgcacc
      481 gcagcaaaaa aaagtaaccc tattcgatca cctcgtcgag gccagagcag attatccgac
      541 ggttgaactt gttccgattg tacttaacga tgaggcctat gcccgccata gcgttcgatg
      601 tcgggtgaga caatgcgctt tgccttgcga acgctgtgcg gatcggcggt cttgacgccg
      661 ataaccggcc caccgcgaat agttatgcgc tccatgaatc gtcgctgagg atagtagtcg
      721 ttgacggcat aatgctgggt cgacctgttg tcccagatcg caatggtgtg cggagcccag
      781 tgaagccgga actgatattc aggaatgagt gcctggtgaa atagcgtgtc gagcagggag
      841 cggctctcgc gctcatccat gtccttgatg gcgatggtga actgaggatt gacgaacagt
      901 atattgcgac ccgaaacagg atgaacgcgc accacagggt gcagctgtgg tggatataac
      961 agctcaaagt gctgccggtt cttgcggtct tcctcagtat tatcaaacag cctcttaaat
     1021 ggcttccaat cgtgaatggc ctcgagccca gatatgaatt gctgcatgcg cccgctgaga
     1081 ccgtcgaacg ccgccgacat gctcgcgaac atggtgtcgc cgccgattgc cggcaccacc
     1141 tttgcgcata ggatcgtcgc catcggcggc tcggcgcgga acgtttcatc cgaatgccat
     1201 acgtccgtcg cgaacggcgg attgttttcg tcatttctga atttgatgag gacaggcgcc
     1261 ttctcctcgt gtggggcgaa gggatgaaca gtcaattcgc cgaaccgccg gccgaaatcg
     1321 atcagatttt cagatgtgat ttcttgattg cgaaagatga ccagctcgtg ctcgatcaga
     1381 gcgttctgga tcacattgaa tgcctcgtct gataagggtt gacgaagttc gacaccgctg
     1441 acctcggcgc cgagactggc cccgagccgg gtcaccgtga caccgctgca ttcgacggtg
     1501 atgggccgac cctgccaaga gtccaccggt ttattcatga gcgtcctcct ctggaccgat
     1561 gtccgccccg tcgggaatga tctccggaga cgcgctgtcc cgtcggcgga tgcggttcaa
     1621 ggccttttta ccaccaaagc acgtggcatt gctctcgcct cttcactggg gctcgatctt
     1681 gtgctcgcgc gcgatcgccg accatttcgc gcgctgccca tccatgaccg tcgcgaactc
     1741 ggcaggcgtg gtgccgcgtg cgatgagccc cacattcgtc agccgctgtt ggatctccgg
     1801 ctcattgagg atgaggcgcg catcggtggc gatccgttcc tgcaggtccg ccggcatatc
     1861 ccgcggaccg aacagaccca ggaacccgcc gaacgtgaac tccgggtaac ctgcttcacc
     1921 aacggttgga acgttgggcg cggccggtgt gcgcaccgca ttggtgaccg cgagcagttt
     1981 gatcttgtcc gcttgcacgg ctcccagcac caacgccagc ggcatcacgg cgatgtggat
     2041 gcgaccttcc gacaggtcgg aaatcgcgct gaccggattg ttgtaggcca cgaaagtggt
     2101 tgtcattccg acgcgctgtt gaaatgcgag gtaagcgagg tagggcgctc cggggacggc
     2161 atagaaattg agcttcgtcg gctgttcgcg cgccaacttg acgaactccg cgagcgagcc
     2221 gatctccagc ctgggcgtga ccacgacgct caagaagtcc tcgaccgcga gcgagatcgg
     2281 cttgacgtcg ccgatcgggt cgtaggggac cctcgcccgc aacaacggaa cgaccgtgaa
     2341 cgtgctgtga gtggtaaaca gcagcgcatg gccgtcccgc atctcgagga acgcttgcgt
     2401 cccgatgatc gtatcggccc cgggccggtt ctcgaccacg acgggctgct tccagcgctt
     2461 ggcaagggcg tccgccagcg cccgcgctgc cacgtcgacc gagatgcccg ggctgaacgg
     2521 cgtgatgatt cggaccgtgt gttcgggcca actgccggca aaggattgcg ccgacttagt
     2581 gagcgcatag ccgaacacgc caatggcggc ggccaccatc gctttgagga gccatccagc
     2641 agcatggagc gctggagaac ggccgacctg taacgtcccg cgcatggcaa acctcctctg
     2701 caggccggag ctcgtccggc cgcgcaacac ggcgggcggc tcgggcgccg aagcttgacg
     2761 cgtcggagga caggcgtcgt gaaaaaacca tgcgggaggg ctgaaaaaat gctgaaatcg
     2821 acctgaattt cctgctcggg cggctggcca gagggcttcg atggataccg ccgtgcacag
     2881 ggcgttccgg ttcgcgggct acacgctcga catgacgcgc ggcacgctgc gcaacggcga
     2941 gcacgcgatc gagctgcgcc ctaagagctt cgaagtcctc cgctacctcg tggaaaatgc
     3001 cggccggctg atctcaaagg acgagatcat tcaagcggtc tggtcgaacg tggctgtttc
     3061 ggacgagtcg ctgacgcgat gcatcagcga cgtccgcgct gcgctcgccg atgagcacca
     3121 gcgcgtcatc aagaccgtcc cgcgccgcgg ctatatattc gacgtgccgg tattggaaac
     3181 cggcatgccc ggcgcggcag cggccggcgt cgggagtcca gagacgagcg cgccggccga
     3241 gcaggcgtcc ggccgggcgc ggaaatcgtg gccctggttg gcggccgccg ccgcgcttgt
     3301 cttggtgctc gcggtcgttg ccgcagccgt atggctctgg cgtcaaccgg ccggcttgcc
     3361 gctacccgac cgaccctcca tcgccgtcct gccgttcatc aatatgggca tggacccgcg
     3421 gcaggattat ttcagcgacg gcatcaccga ggacatcatc accaatctgt cgaaattctc
     3481 cgaccttttc gtcatcgccc acaactcgac cttcgcctat aagggcaaga gcatcgagcc
     3541 gaggcagatc ggtcgcgagc tcggcgtgca ctatctcctg caggggagcg tacgccgcca
     3601 accggaccag ctgcgcatca ctgcccagct catcgatgcg accaacagcc gacagatttg
     3661 ggccgagcac tacgatcggg tgcccgcgga tatttttgcc attcaggacg aggtcacgca
     3721 aaagctcatc gtcactctcg tcgcccgcat cagcaaatcg gagttcgatc gtgtgttgcg
     3781 caagccgccg caaaccttcg ccgcttacga ttactatctt cgcggcaacg ccatggtcga
     3841 ggaagctttt cgcggcaagc ggggagaaaa catcgcagcc gcgcgcgcgt tcttcgaaca
     3901 agccattgca tccgatccac gctatgcggc ggccgtgcag gctttggccc gcacctattt
     3961 gctcgcatgg ctggagccga ccgcgtatga cccgatcgcc cgcgaatatc gccagcaggc
     4021 cacgctcgac cgtgcgctat cgttggcgca agaggcggtc gcacttgatg ggaatctgcc
     4081 ggaggcccac accgcgctcg cgtgggttct gcattggcac taccgcagag ccgaaagcat
     4141 ggcggagtac cagcgggcgt tcgaattgaa cccgaacctc gccgagcgaa gcttcgctcc
     4201 tgctctctat cataacggtc gcacgcggga ggcgatcgac tacatgcgac ggatcatgcg
     4261 cctcgatccg tttcacccca cgcagctgtt cgtcaatttg ggcgctgcgt tctacctcag
     4321 cggttcgtac gccgaggcgc tcggcccgct ccgcaccgcc accgaccgca tgcccgactt
     4381 ccggccaggg ttcgtttggc gtgcagccgc cgcggcgcag cttggattgg acgaggaggc
     4441 gcgccgggcc gcgtccggcg ttctgcacct gcagcccgat ttcaagatct ccgaatggct
     4501 gcggttcctc aggctggcga agcaggagga cgccgatcgc ttggcggacg gcttacgcaa
     4561 ggccgggttg ccggagtgac tgcaccgttc gagatcgaat ctacgcgaga gatgtagagc
     4621 gggcggcaca cgatgccagt acctccgatc ttccccgtta tgtccttatt gggttacgag
     4681 ctggcctaat gctgtccttc ggcctgggtc cgcttcggcc ccagaagtgg acatgtcgag
     4741 gtgacctcgc gcatgtccgc tatgtgccct gcccaggacc ggactcatgc atcgcagcaa
     4801 cggaatttca atccggccta tgtcggctgg gtgtcaaaaa cggcagggca attgcgatcg
     4861 gacgccggtc taagtccgaa acgcccctaa gagcggacag aatttgctga gctcggcgat
     4921 cggtagatcg atccgcacgc aaagctgccg tgtggagcgt cgaaattgtc cgtgtgcggt
     4981 catcgcgtcg gctggatgcc gagcgccttg gcgatggtgg cgatctgggc gcgctggcgt
     5041 tcgatcgagg cggcgaactc ctttgctccg ccgcgagatg gcgtctgcgc ggtcgcattg
     5101 agcttgtcgt cgatggcctt gtcgttggac accgcgacga cgtcggcgcc gatgcgctca
     5161 cgcaattccg ccgacagggc cttgcttccg aacaggccga cattgccgtc catttcgagt
     5221 tcggggaaac cggcttcgat gccggtcggg atgtcgggca ggctcggtgc ccgctcgcgc
     5281 cccatctgcg ccagcaccgt gatcctgccg gcctcgcgtt gcgggcgcag gatggcgtac
     5341 gaggccgagt agacctgcag gcgatcctcg gcgagatcgt taacggcctg c
//
LOCUS       DATLJT010001559         2966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3827537,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001559 DATLJT010000000
VERSION     DATLJT010001559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827537"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1112
                     /locus_tag="VM910_35755"
     CDS             84..1112
                     /locus_tag="VM910_35755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI67898.1"
                     /translation="MSSSRTDMLKSALAERLWPRPPLWVGAFGAAALALLLAYPFIFT
                     TPFADHLMILILLYALMAQSWNVVAGFSGQISLGQAIFFGIGAYSSTVLFTKYGITPW
                     AGLIVGMLLSALAAVAIGIPTLRLRGHYFAIATLLIGASVQIVFQRWDWVGAASGLYV
                     PIERASPWFYLQFHTSKVPYYYLALGAAAAGYLAVWKLRRSRLGFRLQALRDEPDAAA
                     SLGIAIARHKVMAFMISAAMMSVAGTFYAQYVLVLDPERLFSAEISIIVLLMTVLGGS
                     GTLWGPALGAAILVPLSEYSRIWLGGTGRTIDLILYGILILLVCMWRPSGILSLLEER
                     IRRRAGTP"
     gene            1109..1837
                     /locus_tag="VM910_35760"
     CDS             1109..1837
                     /locus_tag="VM910_35760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67899.1"
                     /translation="MSPLLDVQGLTKRYGGLTANADITFDVNEGEIVGIIGPNGAGKS
                     TLFDLITGFQPPSAGRVLLDGRDITGLRPDRIGRLGLARTFQKLKPFVDLTVTENVMV
                     GALARTSDLKTARERALESLSFVDLLEKRNHFARELSTGQRKRLELARGLAMSPRLIL
                     MDEVTGGVDQRSIAGLVALVRELKQGGLTIVMIEHNMQVIMRLADRVLALHAGRAIAF
                     GAPEEVRSDTAVVDAYLGSIDHAA"
     gene            1827..2534
                     /locus_tag="VM910_35765"
     CDS             1827..2534
                     /locus_tag="VM910_35765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67900.1"
                     /translation="MLLEVRDLEVAYGDYQVVWGVSLSVAAGESVALLGPNGSGKSTV
                     VNTISGLLAAKAGEIRFDGASLKGRPAHVRASLGIAHVLERRRVFPHLTVLQNLTLGA
                     WHAAAKAARPRSLERIFTLFPRLSERKSQFARTLSGGEQQMLALGRGLMALPRLLMGD
                     EPFLGLAPRVVEQMKSVFQALQAEGIAILFIEQNVRLALSMAGRGYILESGHLAIEGD
                     APTLINSPEVRRIFLGR"
BASE COUNT          520 a          937 c          926 g          583 t
ORIGIN      
        1 tcctacaaga cgttgatcgt gttcgcactc tatcttgcgg tggtcatttt caggccgcac
       61 ggcctgttcg gccggttcta gcaatgtcca gcagccggac ggacatgctc aagagcgcgc
      121 tggccgagcg cctttggcca cggccccccc tctgggtcgg cgcattcggt gcggcggcgc
      181 tcgcgctatt gctcgcttat cctttcatct tcaccacacc gtttgctgat cacttgatga
      241 ttcttatctt gttgtatgcc ttgatggccc agtcgtggaa cgtggtggcg ggtttctcgg
      301 gccagatcag cctcgggcag gcgatctttt tcggaatcgg cgcgtactcc tcgaccgttc
      361 tcttcacgaa atacggcatc acgccttggg cgggattgat tgtcggcatg ctgctgtcgg
      421 cgctggccgc cgttgccatc gggattccga ccttgcgcct gcgtgggcac tatttcgcga
      481 tcgctacgct gctcatcggc gcgagcgtgc agatcgtctt ccagcgatgg gactgggtag
      541 gcgcagcgtc cgggctttac gtgccgatcg aacgcgcctc gccctggttc tacctgcaat
      601 tccacacgag caaggtgccg tactattacc tcgcgctggg tgcggcggcg gctggctacc
      661 tggcggtgtg gaagctgcgc cgcagccggc tcggctttcg cctgcaggca ttgcgcgatg
      721 aaccggatgc agccgcaagc ctcggcatcg cgatcgcacg ccacaaggtt atggccttca
      781 tgatctccgc cgcgatgatg tcggtcgcag gcacatttta tgcgcaatat gtgctggtcc
      841 tggacccgga acgcctgttc tcggcggaga tctcgatcat cgtcctcctc atgaccgtgc
      901 tcggcgggag tggcacgttg tggggaccgg cgctgggagc agcgatcctg gtgcccttgt
      961 cggaatacag ccggatctgg ttgggcggca ccggtcgcac gatcgatctc atcctctacg
     1021 gcatcttgat cctgctcgta tgcatgtggc gcccgagcgg cattctcagc ctgctggaag
     1081 agcgcattcg acggcgcgcg ggaacgccat gagcccgctg ctcgacgtcc aaggcctgac
     1141 gaagcgctac ggcgggttga ccgccaacgc cgacatcacc ttcgatgtga acgagggcga
     1201 gatcgtcggc atcatcggtc cgaacggcgc cggtaaatcg acgctgttcg acctgatcac
     1261 cggtttccag ccgcccagcg cgggtcgcgt cctcctagac ggccgcgaca tcaccggcct
     1321 gcgccccgac cgcatcggcc gcttggggct cgcccgcacc tttcagaagc tcaaaccctt
     1381 tgtcgacctc acggtcacgg aaaacgtcat ggtcggagcg ctcgcccgca cctcggacct
     1441 gaaaaccgcg cgcgagcggg cgctggagtc gctctccttc gtggacctgc tggagaagcg
     1501 caatcatttt gcgcgcgagc tctccactgg gcaacgcaag cggctcgagc tcgcgcgcgg
     1561 gctcgccatg agcccgcggc tcatcctgat ggacgaggtg accggcggcg tcgaccagcg
     1621 ctccatcgcc ggcttggtcg cgctcgtgcg cgagctcaag caggggggac tcaccatcgt
     1681 gatgatcgag cacaatatgc aagtgatcat gcgcctggcc gatcgcgtcc tggcgttgca
     1741 cgcggggcga gccatcgcct tcggcgcgcc ggaagaggtg cgcagcgaca ccgccgtcgt
     1801 cgacgcctat ctcgggagca tcgaccatgc tgcttgaggt ccgcgacctc gaagtcgcgt
     1861 acggcgacta tcaagtggtc tggggcgtgt cgctctcggt tgccgccggg gaaagcgtcg
     1921 cgttgctcgg ccccaacggg tccggcaaga gcaccgtggt caacaccatt agcggtctgc
     1981 tcgcggccaa ggcaggcgaa attcgcttcg acggcgcgag tctcaagggc cggcccgcgc
     2041 acgtgcgggc aagcctcggc attgctcacg tcctcgaacg ccgacgtgtt ttcccgcacc
     2101 tgaccgtgct gcagaatttg accttggggg cgtggcatgc ggcagcgaaa gcagcgcgcc
     2161 cgcgaagctt ggaacggata ttcacgctct tcccgcgtct ctccgagcgc aaaagccagt
     2221 tcgcgcgcac cctttccggc ggcgagcagc agatgcttgc tcttggccga ggcctgatgg
     2281 cgcttccgcg actgcttatg ggcgatgagc cgttcctcgg actcgcgcca cgcgtcgtgg
     2341 agcagatgaa gtcggtgttc caagcgcttc aggcagaagg gatcgcgatc ttgttcatcg
     2401 aacaaaacgt ccggctcgcg ctttcgatgg cgggacgcgg ttatattcta gaaagcgggc
     2461 acctcgcgat cgagggagac gcacccactc taatcaacag ccccgaggta cgacggattt
     2521 ttctcggtag atgagggaca agtgcgaacc acgcgcactc aggccgtgtg ttgagcgaag
     2581 cgggccttaa gccaacccag tctctggcga gttcgaactg agtctctgag ataagaacac
     2641 ggaaattgaa aattcgcgac cagagactag cgcactaatt tcgacgaacg cccccttgaa
     2701 acaagaaaaa tccgccacag agacccgcca ccgtctccgt aagccgcggg aatgtcacgc
     2761 aaactcacgc ggaggggaca tggctcccaa agaccggact gcctggctga cgatgcagtc
     2821 agctgcgaac cagtctccgc agcaaattcc ctgctatacc tcgactcaac gaagacccga
     2881 gaagctgtgt tgacactggg ggccaggtgg attcgggtgt ggtggatgat tcgtcctgga
     2941 tttttgtccg cagcggaacg aggcgc
//
LOCUS       DATLJT010001560         5422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2679298,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001560 DATLJT010000000
VERSION     DATLJT010001560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679298"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..1098
                     /locus_tag="VM910_35770"
     CDS             442..1098
                     /locus_tag="VM910_35770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67901.1"
                     /translation="MARLKFRTDGTTVLFDPSDDPFPPEALSQLPPTLPSQAAEQAQP
                     QLPTEIPPAQAQSHLPAVQSEAAADASSLALELTPALTAADEEFCPRTISFTIPGGGP
                     GNPGVQVTAVEHQENGVKTGTIDFTVDVLGDADLRGLFFQFNEAHLNGLKVTGGDGLI
                     TNTLIQANSVINLGQGAEMNGAASPFDVGIRWGHRDPIPTTSIFRCTSPSQMPRTTSP
                     "
     gene            1056..2984
                     /locus_tag="VM910_35775"
     CDS             1056..2984
                     /locus_tag="VM910_35775"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVI67902.1"
                     /translation="MHFTLSNAANNLTLDDIAHQQFGVKIGAAGGKDAKLTTIAPAAP
                     DAHDDAFAIFEDGAGGLGNPSHVPVGQLFEVIKNSDTDADGDTLTVTAFHAGPSHGTV
                     QIVDGSDADLLPGDAILYTPDADYSGPDSFVYCVSDGHGGQDNATVNVSVAAVADIPD
                     LTYSVALGATINEYLVTVTAKQTDLDSSEFIDRISWTVPGGTPADVSITPVGDVNPGD
                     EPDQIVQTFAVTVPLGQDTKFDLDITAVSKEVSNGDEEQATVAVPIELDFTHNTAQET
                     FSTTNQSIWDPSLPAAFNDDRFIGVGHLAPDGSVVPISDHTSVDIGVAEIGGSYSLAL
                     GLKSSLHATFGDIEATLPYNITIDTSYNVTTDSLLIDPSAALDPSAHFSTHGPGGSYN
                     LDFVFDATLSAFLHPVIGPNLDPTVGPLDLGFNLLSIDSATFTKPLDLPPPPDTVAHL
                     TLNWPHSMGASTLETNGAPVDADTLHSDGTSLDIVKLDVDVIALALAVLGISPNPLDL
                     GFVDLVELNVNGGVALAQHFDLNSVLDASLTLEDGTSLPFAFGSPLPIIPNASSHDAN
                     HDGQIDFGLHLTPDATLDNKTALDFHIGGDIGILDFNPGPTLFSVGADLDIGEIPIYH
                     PDAFTLGGFNSQDYHFQV"
     gene            3048..4835
                     /locus_tag="VM910_35780"
     CDS             3048..4835
                     /locus_tag="VM910_35780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HVI67903.1"
                     /translation="MSTKSNISGGGTKSNSASSKSELAAALKSCRGSFIGVGLVSSVT
                     NILMLTGPFFMLELYDRVLPSRSIPTLIGLGALAAVLFAFQGLLEWIRGRILVRIGGA
                     LDEILSLRVHDALVRAPLKGKVGGDGLQPLRDLDQVRSFLSSPGPMALFDFPWIPFYL
                     GICFLFHVLIGLAATIGGLILVFLTYLTEVRTRGPAKAAVTFAATRNALAEAGRRNAE
                     VLQAMGMAGRVGAVWARTNSEYMASQRRATDVAGGLGALSKVLRLALQSAVLGLGGYL
                     AIRQEATAGVIIASSILTARALAPVELAIANWKGFVAARQSWRRLTELLTLLPAREEP
                     MALAAPSAELLVEAVSLAAPGSRTFVVRDVNFALKAGNGLGIIGPSAAGKSSLVRGLV
                     GVWAPVQGTIRLDGAALDQWSCEDLGRHIGYLPQDVELFDGTVAENIARFHAQADPPA
                     IIAAAKQAGVNDLILRLPQGYETRIGEGGMALSAGQRQRVALARALYGDPFLIVLDEP
                     NSNLDHEGEEALTKAILGVRTRGGIVIVVSHRATALASVDLILVMKEGKSQAFGPRDE
                     VMAKIIRRFPAPVAPATPLRVVAESGQAS"
     gene            4922..>5422
                     /locus_tag="VM910_35785"
     CDS             4922..>5422
                     /locus_tag="VM910_35785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HVI67904.1"
                     /translation="MGGWAATVELSGAVITSGQVVVDSNVKKVQHPTGGIVGELRVRD
                     GDRVKEGDIVVRLDETQTKANLLIVTKSLDELAARQAREEAEREGAGQVLVPRELLAR
                     FDDPDVAHVVSEERKLFAIRREAREGQKAQLRERIAQLKEEIRGYEAQQVSKDEQIDW
                     IKKELAG"
BASE COUNT         1043 a         1668 c         1685 g         1026 t
ORIGIN      
        1 gcaccgcgcg attgctttcc ttttcttctt tttctctgag tatctagata tagcctttgt
       61 ttcggctgct ttagctgaca ctccttgaga tgcctggaac gacggtggcg cttcgcgcaa
      121 agcactggac tagatcccaa agcactggac tagatccaaa gcatagaact tccgatctct
      181 ccgccaatcc ctggaactcc cgatctctcc gccaggcacc tgattggtca cgctcttccg
      241 cctacgagcg cgtgaaccat gccgcgcagg agcggatatg tccgccggag tgccagacga
      301 gatctgcatc ttcatgtgga ccttcattgg atcgatcggc tcagtcgcgc cgacgcggcg
      361 cgtgccatac tcgagtctgg aaattcccgc gattgatgtt ctgctgcagg gcgctagccc
      421 tgcaaacggg agggcattgt catggcacgg ctgaaattca ggactgacgg aacaaccgta
      481 ctgtttgacc cttctgacga tccctttccg cctgaagcgc tgtcgcaatt gccaccgaca
      541 ctgccgtcgc aggccgcaga acaggcgcaa cctcagctgc cgacagagat accgccagct
      601 caagcgcagt cgcaccttcc agccgtccaa agcgaggctg cggctgatgc gtcgtcactc
      661 gcactggaat tgacgccagc gttgacggcg gccgacgagg agttttgtcc tcgcaccatc
      721 agtttcacga tcccgggcgg gggcccgggc aatccgggcg tgcaggtcac cgcggttgag
      781 caccaagaaa atggcgtaaa aactggcacg atcgacttca ccgtggatgt cctcggcgac
      841 gccgatctgc gtggcctgtt cttccagttc aacgaggctc acctcaacgg ccttaaggtg
      901 accggtgggg atggactgat caccaacact ctgatccaag ccaacagtgt gatcaacctc
      961 ggacagggcg ccgagatgaa cggcgccgcc agtccgttcg acgtcggcat caggtgggga
     1021 caccgggacc caatcccgac aacatcaatt ttccggtgca cttcaccctc tcaaatgccg
     1081 cgaacaacct caccttagac gatatcgccc atcagcaatt cggggtgaaa atcggcgctg
     1141 cggggggcaa agacgccaag cttacgacca tcgccccggc cgcgccggac gctcacgacg
     1201 atgctttcgc cattttcgag gacggcgccg gcgggcttgg caacccgtcc cacgtcccgg
     1261 tgggtcaact gttcgaggtt attaaaaaca gcgacacaga cgcggatggc gatacgctca
     1321 ccgtcaccgc tttccacgcc ggcccctcgc acggcacggt gcaaattgtc gacggaagcg
     1381 atgctgacct tctgcccggg gatgcaatcc tctacacgcc agacgcagat tacagcgggc
     1441 cggacagctt cgtctattgc gtctccgacg gacatggcgg ccaggacaac gccaccgtga
     1501 acgtgtcggt cgcagcggtc gctgacatcc ctgacctaac ctacagtgtc gctctaggcg
     1561 cgacgataaa cgagtatctc gtaaccgtca ccgcgaagca gaccgatctg gacagctccg
     1621 agttcatcga ccgcattagc tggaccgtgc cgggcgggac acccgccgat gtctccatta
     1681 cgcctgtcgg cgatgtcaat ccgggggacg aaccggatca gatcgtgcag acgttcgcgg
     1741 tgacagttcc gctcggccag gacacgaagt tcgatctcga tatcacggcc gtctcgaagg
     1801 aggtgtccaa cggcgacgag gagcaggcga ccgttgccgt cccgatcgaa ctcgacttca
     1861 cccacaacac ggcccaggag accttctcga cgacgaacca gagcatctgg gatcccagcc
     1921 ttccggccgc gttcaacgac gaccggttta tcggcgtcgg ccacctagcc ccggacggca
     1981 gcgtcgtgcc gatatcggat catacatccg tggacatcgg agtggccgag atcggcggca
     2041 gttacagcct ggcgcttggg ttgaagtcca gcctgcacgc gacgttcgga gacatcgagg
     2101 cgaccctacc gtacaacatc accattgaca cctcgtataa cgtaacgacc gactcgctgc
     2161 tgatcgatcc atcggccgcg ctggatccca gcgcccactt ctcgacccac gggccgggcg
     2221 gatcctacaa cctcgatttc gtgtttgacg ccacgctgtc ggcgttcttg caccccgtca
     2281 tcggccccaa cttggatcca actgtcgggc cgctcgacct tggcttcaac ctgctgagca
     2341 tcgacagcgc caccttcacc aagccgctcg atctcccacc cccgccggac actgtcgccc
     2401 acctcacgct caattggccc catagcatgg gcgcctcgac gttggagacg aacggagcgc
     2461 cggtcgatgc tgacaccttg cactcggacg gcaccagcct cgatatcgtc aagctggatg
     2521 tcgacgtgat cgccttggcc ttggccgtcc tcggcatcag tccaaatccg ctcgacctcg
     2581 gcttcgtcga tttggtggag cttaatgtga acggcggcgt agccttggcg caacatttcg
     2641 acttgaactc ggtcctggac gcctcgctca cgctcgagga tggaacatca ctgccgttcg
     2701 cgttcggcag cccgcttccc atcatcccga acgcgtcaag tcacgatgcc aaccacgacg
     2761 gccagatcga tttcggtctg cacctcacgc cggatgcgac gctggacaac aagaccgctc
     2821 tcgacttcca catcggcggc gacatcggca tactcgattt caatccgggt cccacactgt
     2881 ttagtgtggg cgcagacttg gatatcggag aaatccccat ctaccatccg gatgctttta
     2941 cgctgggcgg cttcaactcg caggactacc acttccaggt ctaggcgcgc agcgccggcc
     3001 gcgacacatt gtggtcgtgg ccggcggccc cggcgagaag gcgttaagtg agcacaaaaa
     3061 gcaatatctc cggaggcggt acgaaatcga actctgcgtc ctcgaaatct gagcttgcag
     3121 cggcgctcaa gtcgtgtcgc ggcagcttta tcggtgtcgg cctcgtgagc agtgtgacga
     3181 acatcctgat gctcacgggt ccattcttca tgctcgagct ctacgaccgg gttcttccga
     3241 gccggagcat acctacgctc atcggcttgg gcgcgcttgc ggcggtacta ttcgcgtttc
     3301 aagggctgct ggagtggatc cggggccgaa tcctggtgcg catcggcggc gcgctggacg
     3361 agattctcag cctcagagtt catgatgcat tggtgcgggc gccgttgaag ggcaaggtag
     3421 gcggggatgg ccttcagccg ctgcgggatc tcgatcaagt tcgttcgttc ctgtcgagcc
     3481 cagggccgat ggcgctcttc gattttccct ggataccctt ctacctcggt atttgcttct
     3541 tgtttcatgt gttgatcggc ttggccgcga ccataggcgg cctgatcttg gtctttctca
     3601 cctacctgac ggaggtccgc acccgcggcc cagccaaggc cgcggtgacc tttgcggcaa
     3661 cgcgtaacgc gctcgcggag gccggccggc gcaatgcaga ggtgttgcag gccatgggca
     3721 tggccggccg cgtaggcgcc gtatgggcac gtaccaatag cgagtatatg gcgagtcagc
     3781 ggcgggcgac cgatgtggcg ggcggcctcg gggctctgtc gaaggtgctg cggctcgccc
     3841 tgcaatcggc agttctgggg ctcggcggct accttgccat ccgtcaggag gccacggcgg
     3901 gagtcatcat cgcgagttcg atcttgacgg cgcgggcgct ggcaccggtc gaactggcga
     3961 tcgccaattg gaagggattt gtcgcggcgc gccaaagctg gcggcggctg acggagctat
     4021 tgacgctgtt gccggcgcgg gaggagccga tggcgctggc ggcaccgagc gccgaactct
     4081 tggtggaggc cgtgagtttg gcggcgccgg ggagccggac cttcgtggtg cgggatgtca
     4141 actttgcgct caaagcagga aacgggcttg gaatcatcgg gccgagcgca gcggggaagt
     4201 cgtcattggt gcgcgggttg gtgggagtat gggcgccggt acagggcacg atccggctgg
     4261 acggagcggc gctcgatcaa tggagctgcg aagatctcgg gcggcacatc ggctacctgc
     4321 cccaggacgt ggagctcttc gacgggaccg tggcggagaa cattgcccgt tttcatgcgc
     4381 aggcggatcc accggccatc atcgcggcag ccaagcaggc gggcgtgaac gatctgatcc
     4441 tgcggctacc gcaaggctac gagacacgga tcggggaagg gggaatggcg ctctcggccg
     4501 gccagcggca gcgcgttgcg cttgcgcgcg ccctctatgg cgacccgttc ctgattgtgc
     4561 tggatgaacc aaactcgaat ctcgatcacg aaggcgaaga ggcgttgacc aaggcgattt
     4621 tgggagtgcg gacgaggggc ggcatcgtga ttgtcgtctc gcaccgggcg accgcgctgg
     4681 cgagcgtcga ccttatcctg gtgatgaaag agggcaagtc tcaggccttc ggtccgcgag
     4741 acgaagtgat ggccaaaatc atccgccgtt ttcccgctcc agtggcgcct gcaacgccgc
     4801 tcagggtggt tgccgagagt ggacaggcat catgacgcgg aagatccaaa cgaagagcca
     4861 cgagtcgctt cgccgccacc tgctttccgc cgcggccctt accgtcattc ttgtctttgg
     4921 aatgggcggt tgggccgcca cggtggaact ttcgggcgca gtgatcacgt ctgggcaggt
     4981 agtggtcgat tcgaacgtca agaaggtgca acacccgacg ggaggcatcg tcggagagct
     5041 gagggtccgc gacggcgacc gcgtcaagga aggagatatc gtggttcgtc tcgacgaaac
     5101 gcagacaaaa gcgaacctgc tgattgtgac gaaaagccta gacgagcttg ctgcgcggca
     5161 agcacgagag gaagccgagc gggagggggc cggccaagtt ctcgttccgc gcgagctcct
     5221 cgcgcgcttt gacgatcccg atgtggcgca tgtggtgagt gaagagcgca aattgttcgc
     5281 gatccgtcgc gaggcacggg aagggcagaa ggcgcagctc agggagcgca ttgcccagct
     5341 gaaagaagag atccgtgggt atgaagcgca gcaggtctcc aaagacgagc agatcgattg
     5401 gatcaagaag gagctcgcgg gg
//
LOCUS       DATLJT010001561         1553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2218416,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001561 DATLJT010000000
VERSION     DATLJT010001561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218416"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VM910_35790"
     CDS             <1..832
                     /locus_tag="VM910_35790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI67905.1"
                     /translation="TADGYGRGRNEELIGEAVREHRGDYVIASKFGNLGLLGDPRFAD
                     GRPEYVREACEKSLGRLKTDVVDLYYIHRVDPTVPIEDTIGAMSELVREGKVRFLGIC
                     EAGAATIRRAHAVHPLAAVQIEYSLWTRDVESEILPLCEALGIGFVAYSPLGRGLLTG
                     SVTTADAFGASDVRRNMPRFQGDNLRRNLLLVEELKAHAQAENCTPAQLALAWLLAQK
                     PFVVPIPGTSHARRLEENAAATALSLSADTAEVVARVFAPGAAAGLRYPENHLARLGI
                     "
     gene            complement(1219..>1553)
                     /locus_tag="VM910_35795"
     CDS             complement(1219..>1553)
                     /locus_tag="VM910_35795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67906.1"
                     /translation="MPAPLEMPISLFRLGERECPVGAPLVLPGIDDDPGDAFDPDEVI
                     LDGFDARDIFGDDAERSRSLSSKTTPLRSTTPSFTVIERPSRGTQTTFASLASPDRWR
                     KRKVSRRQ"
BASE COUNT          275 a          484 c          505 g          289 t
ORIGIN      
        1 caccgccgac ggctatggac gcggccgcaa cgaggaactc ataggcgagg cggtccgaga
       61 gcaccgcggc gattacgtca tcgcgagcaa gtttggcaat ctcgggctac tcggcgaccc
      121 gcgcttcgcg gacggccggc ctgaatatgt gcgcgaagcc tgcgagaaga gcctcgggcg
      181 gctcaagacc gacgtcgtcg acctctatta catccatagg gtcgatccga ccgttccgat
      241 cgaggacacg atcggcgcga tgagcgagct tgtccgcgag ggcaaggtac gctttctcgg
      301 catctgcgag gccggggcgg ccaccatccg gcgggcgcat gcggtgcatc cgctcgccgc
      361 cgtgcagatc gaatattctc tgtggacgcg cgacgtggag agcgaaatct tgccgctttg
      421 cgaggcgctt ggcatcggct ttgtcgccta cagtcccttg ggtcgcggcc ttctgacggg
      481 aagtgtcacg accgccgacg cattcggtgc cagcgacgtg cggcgcaaca tgccgcggtt
      541 tcagggcgac aatctgcggc gcaacctcct cctcgtcgag gaattgaagg cgcacgcgca
      601 ggcggaaaac tgtactccgg cgcaattagc gcttgcctgg ctgttggcgc agaagccgtt
      661 tgtcgtgccg attccaggga cgagccacgc ccgccgcctt gaagagaacg ccgcggccac
      721 ggcactttcc ctttccgccg atacagcgga ggtggtcgcc cgggtgttcg cgccgggcgc
      781 cgcagccggg ctgcgctacc cggaaaacca cctcgcgcgc ctcggcatct gaacgcgggt
      841 cttgccaggt cccggaagga ccggttcgat caggctcccg gatgaccaca tcttggtacg
      901 catttgacgc gcgccaacgc gagagggacc gaaagttcgc agaaccgaag cctgtcaacc
      961 ggaattcgaa tccagttccc tccagcggcg agtctgcaaa ctttcggttc ctagccgaat
     1021 gataaagaaa ggtatacttg gatcgtccca tctcgatgcc atggtgaatg ccttggtgct
     1081 ggtgcccggc cggcgcgctc ggcgcggcaa ttgtcgctgg agtcctcttg acgttaccct
     1141 gagaagtgtc aacggtcttc ccttcgaatg cgcagcgccg cattggtcat cgccggcctc
     1201 gcgccgcgct cagtatcttc attgtctgcg acttactttt cttttccgcc agcgatcagg
     1261 cgacgcgagg ctggcgaaag tggtctgggt gccgcgggac ggcctctcga tcacggtgaa
     1321 ggatggcgtc gtggacctca atggcgtcgt tttggaggaa agagagcgcg agcgctccgc
     1381 gtcgtcgccg aaaatgtcgc gggcgtcaaa gccgtcgagg atcacctcgt ctggatcgaa
     1441 cgcgtctccg ggatcgtcat cgatccccgg caggacgagc ggggccccga ccggacattc
     1501 gcgttcgccg aggcgaaaga ggcttatcgg catttcgaga ggcgcgggca ttt
//
LOCUS       DATLJT010001562         2979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2447994,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001562 DATLJT010000000
VERSION     DATLJT010001562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447994"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VM910_35800"
     CDS             <1..259
                     /locus_tag="VM910_35800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HVI67907.1"
                     /translation="RGVIAPGYKADVNVIDYDNLHLHPPKVHYDLPVGGRRLLQQVDG
                     YEATIVSGHITRRAGSATGARPGRLVRGAQGEARQFGTAAG"
     gene            complement(341..805)
                     /locus_tag="VM910_35805"
     CDS             complement(341..805)
                     /locus_tag="VM910_35805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HVI67908.1"
                     /translation="MSVSVIQQAKIQAQILVPLVKALQTELGEARANALVRRALGDIY
                     RRYGEEFWRAKNETHLGKAMTSAFATYARDDALDYKVVEQSQDAFGIDVTGCRYADFY
                     KELGEPELGFLLVCTADFSVAEGFGGDIKLTRTQTIMQGASHCDFRYRRQKD"
     gene            complement(832..2154)
                     /locus_tag="VM910_35810"
     CDS             complement(832..2154)
                     /locus_tag="VM910_35810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67909.1"
                     /translation="MAAQATPVATPVDSVERTVGGTPRGAWKITFLLFLFMLVNFADK
                     IVVGLAGGPIMDELKISPQQFGDLGSSFFYLFSASAIIVGFIVNRVPTRWVLLVMGLV
                     WALAQFPMVGTIGLTTLFICRIILGAGEGPAFSVAVHAIYKWFPDEKRTLPTAILSQG
                     SAFGVIIAVPALNWLIVQFSWHYAFGALGVVGLLWVVAWLFLGKEGPLTAKTTTAADQ
                     ARVPYLQLLTSRTFIGCCAATFGAYWALSLGLTWFTPFIVKGLGFSQTNAGWISILPW
                     LFGACIVLLTGWLSQVLISRGYTTRVARGILGATPLVFGGAIIAVLPHVHSTGLQIAL
                     LVFGSGLCGSIYVVCPPMLGEFTPVSQRGAVLAIYGAIYTMAGIVAPSVMGRVIQSSA
                     NPLDGYMTGFTINAVILVSSGVLGLLLLWPNTERARLMAQERAQPKFA"
     gene            2434..>2979
                     /locus_tag="VM910_35815"
     CDS             2434..>2979
                     /locus_tag="VM910_35815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI67910.1"
                     /translation="MLSVQNLTKTYRTASETIAVLRGVNLEVARGERVALTGESGSGK
                     STLLHLIAGLDSADSGEIRLDETSVTALSDAGRAALRRDRLGLVFQQFNLIPSLNVAD
                     NLAFQARIAGRHDAAWHAELVERLGLASLLKRYPEQLSGGQQQRVAIGRALAPKPLLL
                     LADEPTGNLDEDTADDVLALAR"
BASE COUNT          587 a          966 c          953 g          473 t
ORIGIN      
        1 ccgcggcgtg atcgcgcccg gatataaggc cgacgtcaac gtcatcgact acgacaattt
       61 gcacctgcat ccgccaaagg tgcattacga tctgccggtc ggcggcagac gcctgctgca
      121 gcaggtcgac ggctatgagg caaccatcgt ctcgggccac atcacccggc gcgccggctc
      181 cgccacgggc gcacgccctg gaaggttggt tcgcggtgcg cagggcgaag cgcgtcaatt
      241 cggtacggct gccggctgat agcggctcac atcgcaccgt gtccaccgtt aagggccggg
      301 ttggggtggt gagtggctgc gtccgcgccc cgcctgccgc tcaatccttc tgccgcctgt
      361 agcgaaagtc gcaatggctg gcgccctgca tgatggtctg tgtacgcgtg agcttgatgt
      421 cgccgccgaa gccttctgca accgaaaaat ccgcagtgca gaccagcagg aatccgagtt
      481 ccggctcgcc gagctccttg tagaagtcgg cgtagcggca ccccgtcaca tcgatgccga
      541 aggcatcctg cgactgctcg acgactttgt aatcgagcgc atcgtcgcgg gcgtaggtcg
      601 caaatgcgga cgtcatggcc ttgccgagat gggtctcgtt cttcgctcgc cagaattcct
      661 cgccgtagcg gcgatagatg tcgccgagcg ctcttcgaac aagcgcgttt gcccgcgctt
      721 cgcccaattc ggtctgaagc gccttcacca gcggaaccag gatctgagcc tggatcttgg
      781 cctgttgaat gaccgatacg ctcacgctcg acctcgttag ggctggcggg ctcaggcaaa
      841 cttcggctgc gcgcgctcct gcgccatcag ccgcgcgcgc tcggtgtttg gccagagcaa
      901 caacaggccg agcactccgg acgaaaccag gatcacggcg ttgatcgtga agcccgtcat
      961 gtagccgtcg agcggatttg ccgacgactg gatgacccgg cccatcaccg acggcgcgac
     1021 gatgcccgcc atggtgtaga tcgcgccata gatcgcaagc accgcgccgc gctgcgagac
     1081 cggcgtgaat tcgcccagca tcggcgggca gactacgtag atcgagccgc acaggccgga
     1141 gccgaacacc agaagcgcga tctgcaggcc ggtgctgtgg acatggggca gcaccgcaat
     1201 gatcgcgccg ccgaagacca aaggagtcgc accgaggatg ccgcgcgcca cgcgcgtggt
     1261 gtagccgcgc gaaatcagga cctgcgacag ccagccggtg agcagcacga tgcacgcgcc
     1321 gaagagccag ggcaggatcg agatccagcc ggcattggtt tgcgagaagc cgagcccctt
     1381 gacgatgaac ggcgtgaacc aggtcaagcc cagagacagc gcccaatagg cgccgaatgt
     1441 cgccgcacag cagccgatga aggtgcgcga ggtcaaaagc tgcagataag gcacgcgcgc
     1501 ctggtccgcc gcagtcgtcg tcttggccgt caacggcccc tctttgccca ggaacagcca
     1561 cgccacgacc cacaacaggc cgacgacgcc gagcgcgccg aaggcgtaat gccaactgaa
     1621 ttgcacgatc agccagttca gcgccggtac cgcaatgatg acgccgaacg ccgaaccctg
     1681 cgacagaatc gcggtcggca gcgtgcgctt ctcgtcgggg aaccatttgt agatggcgtg
     1741 gaccgccacc gagaatgccg gtccctcgcc agcgcccagg atgatgcggc agatgaagag
     1801 cgtggtgaga ccgatcgtgc ccaccatcgg aaattgcgcg agcgcccaca cgaggcccat
     1861 gaccagcagc acccaacgcg tcggcacgcg gttgacgatg aagccgacga tgatcgccga
     1921 cgccgagaac aggtagaaga aggaggagcc gagatcgccg aactgctgcg ggctgatctt
     1981 gagttcgtcc atgatcggtc cgccggcgag gccgaccacg atcttgtcgg cgaagttgac
     2041 cagcatgaac agaaacagga gaaacgtgat cttccaggcg cccctgggcg ttccgcccac
     2101 cgttcgctca acgctgtcga ctggtgtcgc cacgggcgtc gcttgcgcgg ccatcgtttc
     2161 ccctccgatc cctttttaag ccctgttggt cgggcggccg gggatgctag gcatgccttg
     2221 cgacgcgacg caacggtgta tttcagcgca ggtcgccggc acggcagccg gtcagtaacc
     2281 tgggcacccc tcaaaaaaac gccctcgacg gatcacgttc gcgtcggcga tacgcgagaa
     2341 acagtgatcg gacgggatga ccttgctatg ctggcccaaa gcgacaatgg aggcctcatt
     2401 ggctggcccg gcaaggcaaa cggcaacgcg atcgtgctga gcgtacaaaa cctgaccaag
     2461 acataccgca cggcctccga aaccattgcc gtactgcgcg gggtcaatct cgaagtcgcg
     2521 agaggcgaac gcgtggcgct gacgggcgaa tccggttcgg gcaagagcac cctgttgcat
     2581 ctgatcgcgg ggctcgattc cgccgacagc ggcgagatca ggctcgatga gacgagcgtg
     2641 accgcgctct ccgatgcagg tcgggctgcg ctgcggcgcg accggctggg actcgtattc
     2701 cagcagttca atctgattcc gagtctcaat gtcgccgaca atctggcgtt ccaggcacgc
     2761 atcgccggcc ggcacgatgc cgcctggcat gccgagctgg tcgagcgact cgggctggcg
     2821 agcctgctca agcgctatcc cgagcagctc tccggcggcc agcagcagcg ggtagcgatc
     2881 ggccgtgcgc ttgcgcccaa gccgctgctt ctgctcgccg acgagccaac aggaaatctc
     2941 gacgaagaca ccgccgatga cgtgctggcg ctggcgcgc
//
LOCUS       DATLJT010001563         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4515463,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001563 DATLJT010000000
VERSION     DATLJT010001563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515463"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VM910_35820"
     CDS             complement(<1..889)
                     /locus_tag="VM910_35820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI67911.1"
                     /translation="MRGRTAMCFAVGVAAALAAPSARAADNWPSRPIHIVVPYPAGGI
                     VDIVARAVTEQVGRDLKQAIVVEARPGASSNIGTAAVARSEGDGYTWLVTGPAVLVNP
                     SLYKDAGWDPLKDFKCVGLAVWNQSVALVPPSFPVKTIGEFVALAREKPDQLNFGNPG
                     TGSSIDLSAQKLFQAADIKLTNVSYKGQPPALLDLMTGLIHFEIVSLALALPHIKEGT
                     VKPLAVFTEQRVADLPDVPTITEAGFAQAAYMPWYGIYVPSATPDALVEKIHDAIDKA
                     LENPDVQRQLAISDIPGKPM"
     gene            complement(905..1717)
                     /locus_tag="VM910_35825"
     CDS             complement(905..1717)
                     /locus_tag="VM910_35825"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVI67912.1"
                     /translation="MVSATTARPRPKTDQGDPSFATTLAHGLDVLAAFRNASGSLSNA
                     DLAERTGLSRPTVSRLTYTLTQLGYLKRDPKGRFELGLGVLATAYPLLSAIKVRQMAR
                     PLMRDFAAYAGGTVSIAMPLGLDFIYVETLRTTDAVAHNPDVGFSGSIAMTAVGRALL
                     SLYSKDELDAYVARVKAERPHEADAVERRTLPEMELCKQRGFAVSLGEWRREIFGVAA
                     PLYRTPTGDCLAVNCGIPSFRFNAEQIERECGPRILGLARSIRSLAGGQDAT"
     gene            1970..>2695
                     /locus_tag="VM910_35830"
     CDS             1970..>2695
                     /locus_tag="VM910_35830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI67913.1"
                     /translation="MSQPALSESPREKPKDATASVVARQAAVRKALPFSDIADFDDAS
                     RGFLGTIENAKVLSTSGRTVWSLEAYGFLSEAEAPATVNPSLWRQSRLNMNHGLFEVV
                     PGVYQVRGLDIANMTLVEGDSGVIVVDTLTSIEGARAAMELYFKHRGKKPVTAVIFTH
                     THTDHWGGARGVLDDATLASGKVPIIAPNLFMEHAVSENIIAGPAMLRRAQYQFGPFL
                     AKGPRGQVDCGLGKTMAAGGVTLL"
BASE COUNT          521 a          815 c          871 g          488 t
ORIGIN      
        1 gcatcggctt gcccggaata tcggagatcg cgagctgacg ctgcacgtcg ggattctcca
       61 gcgccttgtc gatcgcatcg tggatctttt cgaccagcgc gtcgggagtg gctgacggca
      121 cataaatgcc gtaccagggc atataggctg cctgggcaaa tccggcctcc gtaatagtcg
      181 gcacgtcggg aagatcggcg acgcgctgtt ccgtgaagac cgcaagcggc ttcacagtgc
      241 cctccttgat atgcggcagc gcgagcgcaa gcgacacgat ctcgaaatga atgaggccgg
      301 tcatcaggtc gagcagggcc ggcggttgtc ccttgtagct gacgttcgtc aacttgatgt
      361 cggcggcctg aaacagcttt tgcgcggaaa gatcgatcga tgagccggtg ccgggattgc
      421 cgaaattgag ctgatccggc ttttcgcggg cgagcgccac gaactcgccg attgtcttga
      481 ccggaaacga cggcggcacg agcgcgacgc tctggttcca caccgcgagg cccacgcatt
      541 tgaaatcctt cagcggatcc cagccggcat ccttgtaaag gcttgggttg accagcacgg
      601 caggtcccgt gacaagccag gtgtaaccgt cgccctcgct gcgtgcgacc gcagccgtgc
      661 cgatattgct gctggcgccg gggcgggctt ccaccacgat cgcctgcttc aaatcgcgac
      721 cgacttgttc ggtcaccgcg cgggcaacga tgtcgacaat gccgccggcg gggtagggca
      781 cgacgatgtg gatcggccgg ctcggccagt tgtcggccgc acgggcgctc ggcgctgcaa
      841 gcgccgcggc aacgccgacg gcaaaacaca tggcagttcg tccgcgcatt cttccctccc
      901 gactttacgt ggcgtcttgt ccgcccgcca gcgagcggat gctgcgggca agacccagga
      961 tacgcggccc acattcgcgc tcgatctgtt cggcgttgaa acggaaggat ggaatcccgc
     1021 aattgacagc caggcaatca ccggtcggcg tccgatagag cggcgctgcg acgccaaaga
     1081 tttcacgccg ccattctccc agagaaacgg caaagccgcg ctgcttgcag agctccattt
     1141 ccggaagtgt ccgccgctcg acagcgtcgg cttcgtgcgg acgttccgcc ttcacgcgcg
     1201 ccacataggc atcaagctcg tccttgctat acagcgacaa gagcgcgcgt ccgaccgcgg
     1261 tcatcgcaat cgagcccgaa aagccgacat cgggattatg tgccacggca tcggtggtgc
     1321 gcagcgtctc gacatagatg aagtcgaggc cgagcggcat ggcgatcgaa acggtgccgc
     1381 cggcataggc cgcgaagtcg cgcatcagag gccgcgccat ctgccgcacc ttgatagccg
     1441 acagcagcgg ataggcggtt gcgagcacgc caagtccaag ctcgaagcgg cccttgggat
     1501 cgcgcttgag gtagccgagc tgggtgaggg tgtaggtcag ccgcgagacg gtcggccgcg
     1561 acagtccggt gcgctcggca agatcggcgt tcgacagcga gccggaggcg ttgcggaacg
     1621 ccgccagcac atcgagccca tgcgcgagag tggtggcaaa ggaggggtcg ccttgatcgg
     1681 ttttgggcct tggtcgggcg gttgttgcac ttaccatgca gaaatatcga actaaaatcc
     1741 aattcttgtc aagatatcga aacaaatttg cgtttcgtat cgatatacga tagacgatca
     1801 tcaaagcggg aggaagccat ggacttcgag ctgtccgcgt agtcgcaggc gcggcgacga
     1861 aagctgcgtt gagtccgagg tgctgctgcg tcatcgccgc tatctcgctc atgccgctca
     1921 cccggcgtga tccagttcgt ttgttgcttg ccttgtgagg gaggttgtta tgagccagcc
     1981 ggcattgagt gaaagcccca gggaaaaacc gaaagacgca accgcctcag tggtggcgcg
     2041 gcaggcggcg gtcaggaagg cgttgccgtt ctctgacatc gccgatttcg acgacgcatc
     2101 ccgcggcttc ctcggcacga tcgagaacgc aaaggtgctg tcgacatcgg gccggacggt
     2161 ttggagtctt gaagcttacg gctttctctc cgaggccgag gcacccgcga ccgtcaaccc
     2221 cagtctgtgg cgacagtcgc gcctcaacat gaatcatgga ttgttcgagg tcgtgcccgg
     2281 cgtatatcag gtacgcgggc tcgatatcgc caacatgacg ctggtcgaag gcgacagcgg
     2341 cgtcattgtc gtggacacgc tgacctcgat cgagggcgcc cgcgccgcga tggagcttta
     2401 cttcaagcat cgcggcaaaa aaccggtcac cgccgtgatc ttcacccaca cgcacaccga
     2461 tcattggggc ggcgcgcgcg gcgtgctgga cgacgcgacg cttgcgagcg gcaaggtgcc
     2521 gatcatcgcg cccaatctct tcatggagca tgcggtatcg gaaaacatca tcgcggggcc
     2581 cgcgatgctg cgacgcgcgc aataccaatt cgggccgttc ctcgccaagg ggccgcgcgg
     2641 tcaggtcgat tgcggactcg gcaagacgat ggcggcaggc ggggtcacgc tgttg
//
LOCUS       DATLJT010001564         2667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1529790,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001564 DATLJT010000000
VERSION     DATLJT010001564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529790"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1109
                     /locus_tag="VM910_35835"
     CDS             300..1109
                     /locus_tag="VM910_35835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67914.1"
                     /translation="MQTLHVNGYDMAYLDVGEGPPLVCVHGSLCDFRTWGAVIGPLST
                     RHRVIAVSLRHFFPEHWDGVGNTYSIAQHIDDVIAFIEKLNAGPVDLMGHSRGGHVCF
                     RIAQRRGDLLRRLILAEPGGELDGTLDPDHKPRPSPLLEHFTISAQMIAAGDIDGGLA
                     FFMDTLEGAGTWPRLPGMSKQLLRDNATTLIGQVRDNRLPFSKADAEAITVPTLFIGG
                     ANTKGLLPKVLHSLAAHVANSKTEMIPGATHPMFEQAPQKYSEIVLDFLAG"
     gene            1112..1927
                     /locus_tag="VM910_35840"
     CDS             1112..1927
                     /locus_tag="VM910_35840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI67915.1"
                     /translation="MQTIRVNGYDMAFLEVGKGPPLVCVHGTLGDFRTWYAVLGPLSR
                     KHRVIAVSLRHFFPEHWDGVGDDYRMAQHVADLIAFIEQIEPRPLDLMGHSRGGHIAF
                     RVAQSRPDLLRKLVLAEPGGDLDASLDPAAKPGTPSPFAIRILASVNKVREGDVEGAL
                     QNFVDSIDGEGRWRRLPAAAKQQLRDNATTLLGQAHEARKPYSKADAEAIRTPTLLIG
                     GGDTQGSLATIWRMLAGRVAEARTAVIPGARHWMFEQDPQEYCRVVMEFLATP"
     gene            2102..>2667
                     /locus_tag="VM910_35845"
     CDS             2102..>2667
                     /locus_tag="VM910_35845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI67916.1"
                     /translation="MIPLEPAPNLTDQVYARILEAITDRTLLPGQRVTQNELAERLGV
                     SRQPVSHALHLLHRQGLLAESGRRGFEVTQLDPQRIRQLYEVRGALDALAARMAAGRV
                     RTDATGRAQLEAALRAGRAIDKKTPLARLVALDVDFHSAIYRLAGNPAIEEIIAPQWP
                     HMRRSMATVLAELDYRQSAWSEHETIAA"
BASE COUNT          483 a          860 c          843 g          481 t
ORIGIN      
        1 aaatcggcgc tccccctccc ggctgatgtg agcgagcacc cgtgcattgt tggcgtgcac
       61 cacccagcgc gccatgggca gccagtagaa caccgggtcg agcttgagca catgcgaggt
      121 gtgttcaacg atcgcggtgt cgaacaatgt aaaatgctgg tctgtcatgg ccggctccag
      181 aattgcacgc cccgctcttt aggtttcgga cggaatctag gcttggaaat tgttgcgatc
      241 cacccggatt ccgggaatct tgcacagccg gttccccacc atcgcgcgag tcaccctgca
      301 tgcagaccct tcacgtcaac ggttacgaca tggcttatct ggatgtcgga gaggggccgc
      361 cgctcgtttg cgtgcacgga tcgctctgcg acttccgtac ctggggggcg gtgatcggcc
      421 cgctctcgac gaggcatcgc gtgatcgcgg taagcctgcg gcatttcttt ccggagcact
      481 gggatggcgt cggcaacacc tattcgatcg cgcaacatat cgatgatgtg atcgcgttta
      541 tcgagaagct gaatgcaggg ccggtcgatc tgatgggtca ttcgcgcggc gggcacgtct
      601 gttttcgcat cgcgcagcgg cgaggcgacc tgctgcgacg actgattctg gccgagcccg
      661 gcggcgaact cgacggcacg ctcgatccgg atcacaagcc gaggccatcg ccgctgctgg
      721 agcacttcac gatctccgcg caaatgatcg ccgccggaga catcgacgga ggcctcgcct
      781 tcttcatgga tacgctggaa ggcgcgggca cctggccgcg cctgccgggc atgtcgaagc
      841 aactcctgcg ggacaacgcg accacgctga tcggtcaggt tcgcgataac cgcctgcctt
      901 tttcgaaggc cgacgctgaa gccatcacgg tgccgacgct gttcatcggc ggcgccaata
      961 ccaagggcct gctgccgaag gtgctgcact cgctggccgc tcatgtcgcg aattcaaaga
     1021 ccgaaatgat tcccggcgcc acgcatccga tgttcgagca ggcgccgcaa aaatattctg
     1081 agatcgtgct ggattttctg gcgggctgat catgcagacg atcagggtga atggatatga
     1141 catggccttt ctcgaagtcg gaaaaggccc gccactggtc tgcgtgcatg gaacgctcgg
     1201 ggatttccgc acctggtatg ccgtgctcgg gccgctgtcg agaaagcacc gggtgatcgc
     1261 ggtcagtctc cgccatttct ttccggaaca ttgggatggt gtcggcgacg actacaggat
     1321 ggcgcagcat gtcgccgatt tgatcgcctt catcgagcag atcgagccaa gaccgctcga
     1381 tctcatgggc cattcgcgcg gcgggcacat cgcgttccgc gtcgcgcagt cccgccccga
     1441 cctgttgcgc aagctggtgc tcgccgagcc cggcggcgat ctcgatgcga gcctcgatcc
     1501 cgcggccaag ccggggacgc cctcgccctt cgcgatacgg atcctggcct cggtcaacaa
     1561 ggtcagggag ggcgatgtcg aaggcgcgtt gcagaatttt gtcgacagta tcgatggcga
     1621 aggccgatgg cggcggctgc cggctgccgc caaacagcaa ctgcgggaca acgccaccac
     1681 cctgctcggc caggcgcatg aggcgcgcaa gccctattcg aaggcggacg cggaagcgat
     1741 ccgcacgccg acgcttctga tcggcggcgg tgatacccag ggttcgctgg ctacgatatg
     1801 gcggatgctg gccgggcgtg tcgcagaggc caggactgcc gtgatcccgg gtgcgcgcca
     1861 ctggatgttc gagcaggacc cgcaggaata ctgccgcgtc gtcatggaat ttctggcgac
     1921 gccgtaatcg gcaccacaac ccgctgttac gcccgtccgt aacccgcgac ggcaaccgcg
     1981 ctttgcggcc ctgccgcatt cgcctctcac ttttgacccc gacgacaaac cccttgacgg
     2041 gcctgccgag ccggtttagc gttccgaata cggaattccg tattccaaac ttgagacgaa
     2101 aatgatcccg ttggagcccg cgcccaatct gaccgaccag gtctatgccc ggattctgga
     2161 ggcgatcacc gaccgcaccc tgctgccggg gcagcgcgtc acacaaaacg agctcgccga
     2221 acggctcggc gtctcgcgcc agccggtgtc gcacgcgcta catctgttgc accggcaagg
     2281 cctgctcgcc gaaagcggcc gccgcggctt cgaggtcacg cagctcgatc cgcagcggat
     2341 ccgtcagctc tacgaggtgc gcggcgcgct cgacgcgctc gcagccagaa tggcggcggg
     2401 gcgggtcagg accgatgcca ccggccgcgc gcagctcgag gctgcattgc gggccggccg
     2461 cgcgatcgac aaaaaaacac cgctggcgcg acttgtcgcg ctcgatgtgg attttcacag
     2521 cgcgatctat cgcctcgccg gcaatcccgc gatcgaggag atcatcgcgc cgcaatggcc
     2581 ccatatgcgc cgctcgatgg cgaccgtgct ggccgagctc gattatcggc agagcgcctg
     2641 gagcgagcat gagacgatcg ccgcgca
//
LOCUS       DATLJT010001565         2704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1069358,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001565 DATLJT010000000
VERSION     DATLJT010001565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069358"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VM910_35850"
     CDS             <1..140
                     /locus_tag="VM910_35850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI67917.1"
                     /translation="MVVWALSDNDPATEFYRALGGRMVARSSEKFGPKSLDKVAFAWT
                     N"
     gene            221..751
                     /gene="ppa"
                     /locus_tag="VM910_35855"
     CDS             221..751
                     /gene="ppa"
                     /locus_tag="VM910_35855"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HVI67918.1"
                     /translation="MRIDAIPIGIDPPREVNVIIEVPVGGEPIKYEMDKEAGTLVVDR
                     FLYTAMRYPGNYGFVPHTLSNDGDPCDVLIANTRAIVPGAVMSVRPVGVLLMEDEAGG
                     DEKIIAVPSSKLTQRYDKVKSFGDLPEITLAQIQHFFEHYKDLERGKWVKVLRWGGPE
                     EAHKLIEDGIARARKK"
     gene            complement(824..1708)
                     /gene="folD"
                     /locus_tag="VM910_35860"
     CDS             complement(824..1708)
                     /gene="folD"
                     /locus_tag="VM910_35860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HVI67919.1"
                     /translation="MTARIIDGKAIAAGLRARVADEVARVKHAHGLTPGLAVVLVGND
                     PASEVYVRSKHRETQAAGMASSEHKVAADVSQDELLALIARLNRDAGVHGILVQLPLP
                     KSINAETVINAIDPAKDVDGLHPANAGRLAGGFAALAPCTPLGCIILTRSVHASLEGM
                     NAIVLGRSNLVGRPLLQLLLNENATVTIAHSRSRDLPKLCAQADLVYAAVGRPEMVRG
                     NWIKPGATVIDVGINRLPAGDGKMRLVGDVAFAEVSEVAGAITPVPGGVGQMTVACLL
                     VNTLRAACAIAGLPKPAV"
     gene            complement(1722..2042)
                     /locus_tag="VM910_35865"
                     /pseudo
     CDS             complement(1722..2042)
                     /locus_tag="VM910_35865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
     gene            complement(2079..2369)
                     /locus_tag="VM910_35870"
     CDS             complement(2079..2369)
                     /locus_tag="VM910_35870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HVI67920.1"
                     /translation="MRAVLDIVLIVLDLYIWLLIASAILSWLIAFNVVNTRNQFVSAV
                     AEFLYRITEPVLAPIRNFLPNLGGLDISPIILILIIMFIERVITYYIYPAVF"
     gene            complement(2604..2679)
                     /locus_tag="VM910_35875"
     tRNA            complement(2604..2679)
                     /locus_tag="VM910_35875"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2644..2646),aa:Ala,seq:cgc)
BASE COUNT          502 a          876 c          855 g          471 t
ORIGIN      
        1 gcatggtggt ctgggcactc tcggacaacg accccgccac cgagttctac cgcgctctcg
       61 gcggccgtat ggtggcgcgc tcctcggaga agttcgggcc gaaatcgctc gacaaggtcg
      121 cgttcgcctg gaccaattga ccgcagcgaa agccgcgccg cacgcgcgac ggacgacctg
      181 acacggtttg ttttgattta aggcctcacg gagccgtccc atgcgcattg acgccatccc
      241 gatcgggatc gatcccccgc gcgaggtcaa tgtcattatc gaagtcccgg tcggcggcga
      301 gccgatcaaa tacgaaatgg acaaggaggc cggaaccctg gtggtcgacc gcttcctcta
      361 taccgccatg cgctatcccg gcaattacgg cttcgtcccg cacacgctct ccaatgacgg
      421 cgacccctgc gacgtcctga tcgccaacac ccgtgcgatc gtccccggtg ccgtcatgag
      481 cgtgcggccg gtcggcgtgc tcctgatgga agacgaggcc ggcggcgacg agaagatcat
      541 cgcggttccc tcgtcgaaac tcacgcagcg ctacgacaag gtgaagagtt ttggtgacct
      601 gcccgagatc acgctggcgc aaattcagca tttcttcgag cactacaagg acctcgaacg
      661 cggcaaatgg gtcaaggtgc tgcgctgggg cggccctgaa gaggcgcaca agctgatcga
      721 agacggcatc gcgcgcgcga ggaagaaata ggcaggtcga cgagcgtcga cctctcctct
      781 tccccccgct tccgggcggg agagccgagc ggaatctgcc ggctcacacc gcgggcttgg
      841 gtaatcctgc aatcgcgcag gccgcgcgca gcgtattcac cagcaggcac gccaccgtca
      901 tctggccgac gccgcccgga accggcgtga tcgcgcccgc gacctccgac acttcggcga
      961 aggcgacgtc gccaaccagc cgcatcttgc cgtcacccgc aggcaggcga ttgatgccca
     1021 catcgatcac tgtcgccccc ggcttgatcc agttgccgcg caccatctcc ggccggccca
     1081 ccgcggcgta caccagatcg gcctgggcgc agagctttgg cagatcgcgc gagcgcgaat
     1141 gcgctatcgt aaccgtcgcg ttttcgttca atagcaattg caggagcggc cggccgacaa
     1201 ggttggagcg gccgagtacg atggcgttca tgccttcgag cgaggcatga acgctcctgg
     1261 tcaggatgat gcagccgagc ggcgtgcagg gcgcaagcgc ggcaaagccg ccggcgagcc
     1321 gcccggcgtt ggccggatgc aggccgtcga catccttggc gggatcgatc gcgttgatga
     1381 cggtttcggc gttgatcgat ttcggcagcg gcaactgcac caggatgccg tgcacaccgg
     1441 cgtcgcggtt gagccttgcg atcagcgcca acagctcgtc ctgcgacaca tcggccgcca
     1501 ctttgtgctc ggaggaggcc atgccggcgg cctgcgtctc tctgtgcttg ctgcgaacat
     1561 agacctcgct tgcgggatcg ttgccgacca gcacgaccgc cagccccggc gtcaggccgt
     1621 gcgcgtgctt gacgcgggcg acttcgtccg cgacgcgggc gcgcagacct gcggcaatcg
     1681 ccttgccatc gatgatacgg gccgtcatct aaatccttcg actgtttcgc ttttgcttgt
     1741 gtcagaaccc gcaacgcctc gccaaggcgt ttgggatcgc cgtccaccgc aatctgcttc
     1801 aggcgcgagg tcgcacccga tgcgagcttg accgccgatt tgggcacgcc gagcgccttt
     1861 gcgagaaatt ccgtcacggc gcgattggcc tcgccgttgt ccgcgatcgc gcgtacccga
     1921 attttcagca agctgcggcc gtcgctcagc gtttcgatcc cgtcaatgcc gtcgcggccg
     1981 ccgcgcggcg tcacccgcag gctaacgcag acgccgccgg ccgaggtgcg ccaagggtcc
     2041 atagagggtc catagagcgt ccgtcgcgcc cccgcggatc agaacaccgc gggatagatg
     2101 taataggtga tcacccgttc gatgaacatg atgatcagga tcaggatgat cggcgagata
     2161 tcgagcccgc cgaggttcgg caggaaattg cggatcggtg ccagcaccgg ttccgtgatc
     2221 cgatacagga actccgcgac cgccgagacg aactggttgc gcgtgttgac gacgttgaag
     2281 gcgatcagcc aggacaggat ggcggaggcg atcaacagcc agatgtagag atcaagaacg
     2341 ataaggacga tgtcgaggac ggcgcgcatt ggggactcgg ggctgagatc gcgatttggc
     2401 tccttcgcta gatatcggtt ccaccccaac caaacaaggg aactggtgca gcggattcga
     2461 cttcgctgcc gattttgccc agatcatcgg gcttgacgga ctttcccgtc gccatattgg
     2521 gcgatgcgaa cggtcttcgc tcattcgcta cgaaagactg gagcgtgacc tgccagccgg
     2581 aggctcgcga cgcgagccaa ggctggtgga gccaggcggg atcgaaccgc cgacctcatc
     2641 attgcgaacg atgcgctctc ccagctgagc tatggccccg tacccgccgc cttgtaaggc
     2701 ggct
//
LOCUS       DATLJT010001566         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3140035,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001566 DATLJT010000000
VERSION     DATLJT010001566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3140035"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VM910_35880"
     CDS             <1..780
                     /locus_tag="VM910_35880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVI67921.1"
                     /translation="PMLQLALKKAHDKGIVLIAAAGNAGPRSQPLYPAADENVIAVTA
                     IDADDRLLPQANQGRHIALAAPGVGVLEPAANAGYQLTTGTSVAAAHVSGVAALIIER
                     KPDIDFAALEKILFSTAKDLGPKGRDSQFGFGLVDPYRALDALADKVAADHPTAAAAT
                     SAATAVSETFRVSQLAPGGAPIPAPRSTQPEGDDVRASLEKKRLVCRQEGASKGMRGP
                     DLQDYVVICVAEARLACLKQAVAQKVRGAERRDFLNSCLGS"
     gene            complement(1003..2376)
                     /locus_tag="VM910_35885"
     CDS             complement(1003..2376)
                     /locus_tag="VM910_35885"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI67922.1"
                     /translation="MAVADEAKSAELIARLERLPFSRWHRSFFILAFFGIMFDAADFA
                     LFGAALPPIAREFGLGPAQSGLLATVGLVGAFLGALFWGTVSDYIGRRTSFQATVGIF
                     ALFTALVAASWNVASLAAFRFLSNFGLGGEVPVTSTLASEFSPGRIRGSMTGNIMAAF
                     PVGLIVAAVLSLSIIPTLGWRALFVVGVVPAVLLFFVRRYMPESVRYLLSKGRVADAE
                     KIVADIEQRALGRSLTVEEIRAAPKVKPEVAVPTGVTVFELFTPGRARTTVLVWIVSF
                     GFLWASNGILFMLPTILTQRGIPLTDAIAFQLVQAIGGFFGYSSCSFLIDRYGRRPVL
                     FLYYFVGAFFHLAFAMSTGFATYVTAFLVGWVNPGVYGATSVYVGELHPTHLRATAVG
                     WFFGIGRIGSFLAPSVVGLMLAYGLGGWTLYTFALSYLIVSIALIAVGVETKGRVLEE
                     ITQGKPA"
     gene            complement(2619..>3051)
                     /locus_tag="VM910_35890"
     CDS             complement(2619..>3051)
                     /locus_tag="VM910_35890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI67923.1"
                     /translation="AVEFLKGGKGMPRIVHIAIKVDDLEKATKFYEEVFGFRQTSTGY
                     ARGHVSRHLTDGNIDLALMVYDSEDVEEAQLSGPGPCIHHWGIEVEDRDSYVAKIEAN
                     GGTIISEPGEGALKFRAPDGTVAEIVGAGRYKKKKAEKAPA"
BASE COUNT          653 a          962 c          925 g          511 t
ORIGIN      
        1 ccgatgctcc agctggccct caagaaggcg cacgacaaag gcatcgtgtt gatcgccgcc
       61 gccggcaatg cggggccgcg ctcgcaaccg ctctatcccg ccgcggacga gaacgtgatc
      121 gcggtgacgg cgatcgacgc cgatgacagg ttgttgccgc aagccaatca gggacggcac
      181 atcgcactcg ccgccccggg ggtcggcgtt ctcgagccgg ctgcgaacgc gggttatcaa
      241 ctcaccaccg gcacttcggt cgccgccgct catgtcagcg gggttgccgc ccttatcatc
      301 gagcgcaagc cggacatcga tttcgcggcg ctcgaaaaaa ttttgttctc gaccgccaag
      361 gacctgggtc cgaagggacg cgacagccaa ttcggatttg gtctggtcga tccctatcgg
      421 gcactcgacg ccctcgcgga caaagtcgcc gcggatcatc caactgccgc ggcggcgacg
      481 tcggcggcca cggcggtatc cgaaacattc cgggtcagcc aacttgctcc tggcggcgca
      541 ccgatacccg cgcctcgcag cacgcagccc gagggagacg acgtgcgcgc cagcttggag
      601 aaaaaacgcc tcgtctgccg gcaggagggc gcgagcaaag gaatgcgcgg tcccgacctg
      661 caggactacg tcgttatttg cgtggcggaa gctcgcttgg cttgcctgaa gcaggcagtc
      721 gcgcaaaaag tccgtggagc ggaacgcaga gacttcctca acagctgcct gggatcgtag
      781 cgcgctttct gccggcgttt acggcattgg agcgtcatcc tcccacctcc acgatcgcgg
      841 aagccgattt gctcgcaaga tgtccgctct agcgacggaa ttggcgcgtc gcaacgaact
      901 gtgtcgatgt gccaatcgcg aggtcatgca actgcagtaa gcaaccgccc gtgcgatcac
      961 cgcgtcggcg cgggcgagca gcgtaggcca cgggtcatga cttcaagcgg gcttcccctg
     1021 cgtgatctct tcgagaacgc ggcctttggt ctcgacgcca actgcgatga gggcgatgga
     1081 cacgatcaga taggagagag caaacgtata aagggtccag ccgccgagcc cgtaagcgag
     1141 cataaggccg accaccgagg gtgccaggaa agagccgatg cggccgatgc cgaagaacca
     1201 gccgacggcc gtggcgcgca gatgggtcgg gtgcagttcg ccgacgtaga cgctggtggc
     1261 gccgtatacg ccggggttga cccagccgac caggaaggct gtgacgtaag tggcaaaacc
     1321 cgtcgacatc gcaaacgcca gatggaagaa agcgccgaca aaatagtaca ggaacagaac
     1381 cgggcggcgg ccatagcgat cgatcagaaa gctgcaactc gaatagccga agaagcctcc
     1441 gatcgcctgg accagttgga aagcaatggc gtccgtcagc ggtatcccgc gctgggtcag
     1501 gatcgtgggc agcatgaaca gaatgccgtt tgaggcccac aagaatccga atgagacgat
     1561 ccagaccagc acagtcgtgc gcgcgcgccc cggcgtgaac agttcgaata cggtaacccc
     1621 ggttggaacg gcgacctccg gctttacttt tggcgcggcc cttatctcct cgacggtcaa
     1681 ggaacggcca agggctcgct gctcgatgtc cgcaacgatc ttttccgcat ccgcgacgcg
     1741 acctttgctg agcagatagc gaaccgactc cggcatgtag cggcgaacaa aaaacaagag
     1801 cacggccgga acgacaccga ccacgaacag cgcgcgccat ccaagcgtcg gaatgatcga
     1861 caggctgagc accgctgcga cgatcagccc aaccggaaag gcagccatga tattgcccgt
     1921 catgctgccg cggatgcgtc ccgggctgaa ttcggaagcg agcgtcgacg tcaccgggac
     1981 ctcgccgccc aggccgaagt tcgagaggaa gcgaaaggcg gccagcgagg cgacattcca
     2041 ggaggcggcc acgagggcgg tgaacagtgc aaaaatgccg accgtcgcct gaaacgaggt
     2101 gcgccggccg atgtaatcgg aaacggtccc ccagaataat gcgccgagaa atgccccgac
     2161 caatccaacc gtcgccagca ggcccgattg cgcgggaccg agtccgaact cgcgtgcaat
     2221 gggcggcaat gcggccccaa acaacgcaaa atccgccgca tcgaacatga tgccgaaaaa
     2281 cgccaggatg aaaaaacttc gatgccaacg cgaaaacggc agccgctcca gccgtgcaat
     2341 aagttccgcc gactttgctt cgtccgcgac agccatggtt ttcctcgcgt taagatcagt
     2401 gctacttgca attcgatcgc cagtcttggc cgtcgacgta accagcttcg gctaagtgcc
     2461 ggctccatcc gctccgtcgg ttgggcgagc ctaaaccgag aacctgctca acggctgctg
     2521 cgcgcgccgc gagcggccat gatatagccc ccgccgagaa gcgtgatgaa gctgcggcgc
     2581 tgcattcgat cagctcgctc tccgcgtcat accaccggtc atgccggcgc cttctcggcc
     2641 ttcttctttt tgtagcgacc ggccccgaca atttctgcaa ccgtgccgtc cggtgcgcgg
     2701 aatttcaagg cgccctcacc gggttcggag atgatggttc cgccgttcgc ctcgatcttg
     2761 gcaacgtaac tgtcgcggtc ctccacttcg atcccccagt ggtggatgca cggaccggga
     2821 ccggaaagct gcgcctcctc gacatcttcg ctgtcgtaga ccatcaaggc gagatcgatg
     2881 tttccgtctg tgaggtgtcg cgaaacatgc ccgcgcgcgt atcctgtgct ggtttggcga
     2941 aagccgaaca cctcttcata aaacttggtt gccttctcca aatcgtcgac tttgatggcg
     3001 atgtgaacga tgcggggcat gccttttcct cccttcaaaa attcaactgc g
//
LOCUS       DATLJT010001567         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_381824,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001567 DATLJT010000000
VERSION     DATLJT010001567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381824"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VM910_35895"
     CDS             complement(<1..493)
                     /locus_tag="VM910_35895"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVI67924.1"
                     /translation="MPDTHVLAEHSSQGQLAAYDAIVIGAGISGLYMLYRLRELGMIA
                     RVFEAGTDVGGTWYWNRYPGARFDSESWTYGYSFSKELMREWDWKEHFSPQPDTLAYL
                     NYVADKFDLRRDIQFCSTVAAAHWDEAANRWSVTLESGEQATAQFLFTAIGILSAYTL
                     PAIP"
     gene            complement(929..1828)
                     /locus_tag="VM910_35900"
     CDS             complement(929..1828)
                     /locus_tag="VM910_35900"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI67925.1"
                     /translation="MRPTSNPIYRWRVGEIEIARVLEFEAALFEPTMLYPKATHEIIE
                     RHRTWLEPTLMDPASGLLSFAFYSTVIKTPRATILVDTCSGNDKERPHKLRYHRKNWP
                     YLANLAAAGFAPENIDYVLCTHLHADHVGWNTRLIDGRWTPTFPNARYLFARQEWEHW
                     RVAELRAEYTTDPYYEDSLLPIMESGQAELVATDYAFDDNVWIEPWPGHTPGHVCVVV
                     QCQGASVVLSGDIMHTALQCAEPQFNCCFCVDGEAARTTRRRLLETFADSPVMLIPAH
                     FPTPTAGWIRSRDGSFRFHFDAR"
     gene            complement(1996..>2326)
                     /locus_tag="VM910_35905"
     CDS             complement(1996..>2326)
                     /locus_tag="VM910_35905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVI67926.1"
                     /translation="IRDTWSPYGTADLLERAAIIGWKGDFRRDAEVEVAFDLISAAGA
                     QALGIGDYGITVGAAADLFTIAASCVPEAVAAHPPRKLVFFGGNVVARHGSFLPVPVT
                     LASEASA"
BASE COUNT          462 a          703 c          720 g          441 t
ORIGIN      
        1 ccggaatggc cggcagcgta taggccgaga ggatgccgat ggcggtgaac aggaactgcg
       61 ccgttgcctg ctcgccgctt tccagtgtca cgctccaccg gttcgccgcc tcatcccaat
      121 gcgcggccgc gaccgtgctg caaaactgta tgtcccggcg taggtcaaac ttgtcggcga
      181 cgtaattcag atatgccagc gtgtcgggtt ggggggagaa gtgctccttc cagtcccatt
      241 cccgcatcag ctccttggaa aaggaatagc cataggtcca gctttcggaa tcgaagcgcg
      301 cgccgggata gcggttccag taccaggtgc cgccgacatc cgtgccggcc tcgaagaccc
      361 gagcgatcat gccgagttcg cgcaagcggt agagcatata caggccggaa atcccggcgc
      421 cgatgacgat cgcgtcgtag gccgcaagct gaccctggct tgagtgttcc gccaagacgt
      481 gtgtgtccgg catgacctat tccttcgttg cgacacgcgg ccaaaattcg cgggccgccg
      541 gcgatgcgat cgccggggca atctaccgct tcaacgatca tcaacacggt agctcaaccc
      601 ccatccggta agacaagcaa atgccgaata cccgattggc gccgatcgag accgtaactg
      661 aaccggcgat cgccggatgc tctgcccgtt ggccgccagg atcgcggtga cgatcttgtc
      721 gttgatttgt tctgctcggc acgccattcc atcgcccgag ggagatggcg tcgtgctgac
      781 gatattcttc gaacctcctc atggctgtcc gaactccatt accagttacg gaacgaccac
      841 ggctgctaat caagaggagg ccatttaacc gagcgggtgc caagttttgt gctttgccca
      901 aatctggtga cgagccattg aagctgcttc accgggcgtc gaaatggaat cgaaacgatc
      961 cgtcacgcga tcgtatccaa ccggccgtcg gggtcgggaa gtgagccgga ataagcatga
     1021 cgggggaatc ggcgaaggtc tcgaggagac ggcgccgggt tgttcgcgcc gcctccccat
     1081 cgacacaaaa acagcaattg aactgtggct ccgcgcactg cagagccgta tgcatgatgt
     1141 cgccactcag gaccacggag gcaccttggc attgcaccac aacgcacacg tgaccgggcg
     1201 tgtgtcctgg ccacggctca atccagacgt tgtcgtcgaa ggcataatcc gtagcgacca
     1261 gctcggcttg accactttcc atgatcggca gcaggctgtc ttcatagtag ggatcggtag
     1321 tgtattcggc tcgcaattca gctacccgcc agtgctccca ttcctgacgg gcgaagaggt
     1381 accgcgcatt cgggaacgtc ggcgtccatc ggccgtcgat gaggcgcgtg ttccaaccga
     1441 cgtggtcggc atggagatgc gtgcacagca catagtcgat gttttcggga gcgaaaccgg
     1501 cggcggcgag gttcgcgaga tagggccagt ttttcctatg atagcgcagc ttgtgcggac
     1561 gctccttgtc gttgcctgag caggtatcga ccaggatggt agcccgcggc gtctttatga
     1621 ccgtgctata gaacgcgaag ctgagcagcc cgctggccgg atccattagc gtgggttcga
     1681 gccaggttcg gtgccgttca atgatctcgt gcgtagcctt gggatacagc atggttggct
     1741 cgaataacgc tgcctcaaat tcaagcacac gcgcaatttc gatctcgcct acgcgccagc
     1801 gataaatcgg attactggtg ggtctcaccg gctactcctg ctatgcttga caaggtgagg
     1861 cgctgctccg gcggcactcg aggcaatccg tcgtcgggca tgcggcgggg ccggttccgt
     1921 acggcctata ccggcccgga cgttgcgcaa aaaccttgcc ggctgagggg ccacatcaca
     1981 cgaaggaggc gtgtttcagg ctgacgcctc gctcgctagc gtgaccggaa ccggcaaaaa
     2041 cgatccgtgt cgggcgacaa cgttgccgcc gaaaaagacg agcttccgcg gcggatgggc
     2101 agcgacggcc tcagggacgc agctcgcggc gatcgtgaac agatcggcgg cggcgcccac
     2161 agtgatgccg taatcgccga tgccaagcgc ctgcgcgccg gccgcgctga tcaggtcaaa
     2221 ggcgacctcc acctcggcgt cgcgccggaa gtcccctttc cagccgataa tcgccgcccg
     2281 ttccagcagg tcggcagtgc cgtagggaga ccaggtgtcg cggatg
//
LOCUS       DATLJT010001568         3644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1991575,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001568 DATLJT010000000
VERSION     DATLJT010001568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991575"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VM910_35910"
     CDS             <1..463
                     /locus_tag="VM910_35910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI67927.1"
                     /translation="CRVIAQPLSVALKETVIVENRPGANGALAALYVARSAPDGHTLL
                     MATNSPLSVVPFLMKNVSYDPVKDFAPVTRVGSFTLMLVVNPSLPVKTVKELIEYAKA
                     NPGKLSFASANTSGIVAGETLMHWAEINVLHVPYKSVAGLHGTAYAFVGLA"
     gene            647..1786
                     /locus_tag="VM910_35915"
     CDS             647..1786
                     /locus_tag="VM910_35915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI67928.1"
                     /translation="MRIARGLSLGYRRIETAGPWIVRKATGKGDNWIKNFAHADDFED
                     SNADSVLTFFEAQALARTIARDGETNGGLITVAAAIDRYADDLAARGGRQYNAKLARL
                     HLPPALLGKPVGLLTVRDLKHWRDGLLKGRTAGTVNRTISTVRAALELAAATDPRIIN
                     SAAFKVGLKKLPDAARARNVVLDDAQVRELVRLAYEEDGAFGLLIEVLAVTGARRSQA
                     ARLTVADLQDARPDPRLMMPSSLKGKGVKRIDRRPVPIPAGLAAKLRQGAQGREGLLL
                     LKSTGLPWGSTDHRDPFQRIVVKAGLDPEIVTSYSLRHSSITRALLRGIPIRAVAYAH
                     DTSVPMIEKTYSASIGDHSDTLFRNALLDVDAPVEGNVVPLVRKG"
     gene            1789..2424
                     /locus_tag="VM910_35920"
     CDS             1789..2424
                     /locus_tag="VM910_35920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67929.1"
                     /translation="MVDDVVGGLTDEHRARIEAALPPDKTMSPEAWADLTEIVVGHHL
                     FEQRRTTYPIKKERARWKRLGHALTKGFAAELRQFREAERRQVRDKVPNPMRANRALV
                     ALWEIHCQVEARAAFHAIWSAFSGSKNPHREFLYWGIMRVWTDRLGGELRYSLSPEGL
                     LSGPLVRFLKACLEPVLGDKTPGAGIADIIDRERAARAWVEAEKRRWSDLL"
     gene            2516..2737
                     /locus_tag="VM910_35925"
     CDS             2516..2737
                     /locus_tag="VM910_35925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67930.1"
                     /translation="MTRPEITGRKAPLALSVGEFCDAHDISIGHFYAMLKKGVGPRVM
                     KVGTRTLISVEEAARWRAERTDASHQSEF"
     gene            2865..>3644
                     /locus_tag="VM910_35930"
     CDS             2865..>3644
                     /locus_tag="VM910_35930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67931.1"
                     /translation="MQYTSRKTPKANGFGALRSVLEAARLEIGCGLGDLTVLSAAVDP
                     YRLDTPSGHRDGAWLAKQLDRAIGKKRRIHWRGIHYVLVARGNVVKPNGQTYVNDDDN
                     WTWLINVAGKSARWLGYISFERITDNRNAPPIIHRRARVKVETFVSIGLDVAIPDAND
                     LKPQPIAEGFEPRQAYCFVIFGEKASLEDVLLPIARTKQADLYLPTGEISDTLLHQIA
                     KDANDDGRPLVLFTLADCDPAGHQMAVSIGRKLQAFRDLLFP"
BASE COUNT          673 a         1181 c         1130 g          660 t
ORIGIN      
        1 ttgccgcgtg atcgcccagc cgctcagtgt cgcactcaag gagactgtca tcgtcgagaa
       61 tcgcccgggc gcgaacggcg cgctcgcagc tctttacgtc gcgcgctcgg cgccagacgg
      121 ccatacgctg ctgatggcga ccaactctcc actttcggtc gttccattct tgatgaagaa
      181 cgtgagctac gatccggtga aggacttcgc gccagtgacg cgtgtgggga gcttcacgct
      241 gatgctggtg gtcaatccga gcttgcccgt caagacagtc aaggagctga tcgaatatgc
      301 caaggccaat cccggcaagc tgtcattcgc cagcgccaat acctccggca tcgtcgccgg
      361 cgagacgctc atgcactggg cggaaatcaa cgtgctgcat gtgccctaca agagcgttgc
      421 gggcctccat gggactgcgt acgccttcgt gggcttggcc taagtgcctg ccgcatcgag
      481 caatcccttc actggctttc atacgccctt atagccatct atacccatag ccgggacagt
      541 cccgggacaa cgttgggagg atggcatggc gcgtagtacc cgatcatcgg cgctggaaag
      601 ccgcagcgcg cggctcaagc tgcccaagcg cggcaagccc tactgggtgc ggatcgcccg
      661 cggcctgagc ctcggttacc gtcgcatcga gacagccggc ccgtggatcg tccgcaaggc
      721 gaccggcaag ggcgacaact ggatcaagaa ttttgcccac gccgacgact tcgaggacag
      781 caacgccgac agcgtgctga ccttcttcga ggcgcaggcg ttggctcgta cgatcgcacg
      841 cgacggcgag accaacggcg gcctgatcac ggtggcggcc gccatcgacc gctacgccga
      901 cgatctcgcg gcccgcggcg gccgccagta caacgccaag ctcgcccgcc ttcacctgcc
      961 gcctgctctg ctcggcaagc ccgtcggcct cctcaccgtc cgcgatctga agcactggcg
     1021 cgatgggctc ctgaaagggc gcactgctgg cacggtgaat cgtacgatca gcacggtgcg
     1081 cgcggcgctg gaactcgccg ccgcgaccga tccgaggatc atcaactccg cagcgttcaa
     1141 ggttgggctc aagaagctgc cggacgccgc gcgtgcccgc aacgtggtgt tggacgacgc
     1201 gcaggtgcgc gagctagtca ggcttgctta tgaggaggac ggcgcgtttg gcctgctgat
     1261 cgaggtgctc gccgtcaccg gcgcccgccg ctcgcaggcg gcgcggctca cggtggctga
     1321 cctgcaggat gctcgccccg atccgcggtt gatgatgccc tcgtcgctga agggcaaagg
     1381 cgtcaagcgg atcgaccggc gcccggtgcc gatcccggcc ggcctcgcgg ccaagctgcg
     1441 gcagggggca cagggacgcg aagggctgtt gctcctcaag tccacaggct tgccttgggg
     1501 ctcgacggac caccgcgatc cgtttcaacg catcgtcgtg aaggccggtc tcgatcccga
     1561 gatcgttacg agctactcac tgcggcacag ctcgatcacg cgcgcgctgc ttcgtggcat
     1621 tccgatccgc gctgttgctt atgcgcacga cacctcggtg ccaatgatcg agaagaccta
     1681 ctcggcttcg atcggtgatc actcagacac gctcttccgc aacgccctgc tcgacgtcga
     1741 cgcgcccgtc gaaggcaacg tggtgccgct ggtccggaag ggttgaccat ggtcgacgat
     1801 gtggtcggcg ggctcaccga cgagcaccgt gctcgcatcg aagcggcgct tccacccgac
     1861 aaaaccatgt cgccggaagc atgggccgat ctgacggaga tcgtcgtcgg ccatcacctc
     1921 ttcgagcagc gccggaccac ctaccccatc aagaaagagc gggcccgctg gaagcgcctg
     1981 ggacatgcgc tcaccaaagg attcgcggcc gagctgcgcc agttccgaga ggccgagcgg
     2041 cgccaagtcc gagataaggt gcccaatccg atgcgggcaa atcgcgcgct ggtggcgctg
     2101 tgggaaatcc actgccaagt cgaggcgcgt gcggctttcc acgccatctg gtccgccttc
     2161 agcggcagca agaatccgca tcgcgagttt ctctactggg gcatcatgag ggtctggacc
     2221 gaccgactcg gcggcgagct gcgttattcg ctgtcgcccg agggattgtt gagcggtccg
     2281 ttggtccggt ttcttaaggc ttgccttgag cccgtgctgg gcgacaagac ccccggcgcc
     2341 gggattgccg acatcatcga tcgcgagcgt gccgcccgtg cctgggtcga ggccgagaag
     2401 agacggtggt ctgacctatt atagtgttgt gaccaccaaa aaattttctc acagccccat
     2461 tttttcaacg cgccttcttt gacgttcact cgcctccaac gaaggaggcg agttcatgac
     2521 ccgtcccgaa atcactggaa ggaaggcgcc gctggcgctg agcgtcggcg aattctgcga
     2581 cgcacacgac atcagcatcg gccacttcta cgcaatgttg aagaagggcg tcggcccgcg
     2641 cgtgatgaaa gtcggcactc gcacgctcat cagcgttgaa gaggccgcgc gctggcgtgc
     2701 cgagcgcacc gatgcgtcac atcaatcgga gttttgatct gtcccgtcga ggcgcgctgt
     2761 gccgctcggc gcccgacgga tcgtgactag aagcagcagt cgagacccca ggcgccttcc
     2821 ctctcaaagg ccagcgccgg ggtctcaacc agacggtgga cgtcatgcaa tatacctctc
     2881 gaaaaacccc gaaggcaaac ggcttcggag ccctacgctc cgtgctcgaa gcggcgcggc
     2941 tcgaaatcgg atgcggcctc ggcgatttga ccgtcttgtc agcagcggtc gatccctacc
     3001 ggctcgacac gccatccgga catcgtgatg gcgcgtggtt ggcgaagcaa ctcgatcgcg
     3061 ccatcggtaa gaagcgccgc atccactggc gcggaatcca ctatgtcctt gtcgcccgcg
     3121 gcaacgtcgt caagccgaat ggccagactt acgtaaacga cgacgacaac tggacatggt
     3181 tgatcaacgt cgcgggtaag tcggcacgct ggctcggcta tatttccttc gagcgcatta
     3241 ccgacaatcg caacgccccg ccgatcattc atcgtagggc gagagtgaag gttgagacct
     3301 tcgtgtcgat cggtctcgac gtcgcgatcc ctgacgcaaa tgatctcaag ccgcagccga
     3361 tcgctgaggg cttcgagcca cgtcaggctt attgcttcgt gatttttggc gagaaggcta
     3421 gcctagagga cgtccttctt ccgatcgcgc gcaccaagca ggctgatctc tatctgccga
     3481 ccggcgaaat ctcggacacg cttctgcacc agatcgccaa ggacgccaac gacgatgggc
     3541 ggccgctggt gcttttcacc ttggctgact gcgatccggc tggccatcag atggcggtgt
     3601 cgatcgggcg caagctgcag gcgtttcgcg atctgctgtt tccc
//
LOCUS       DATLJT010001569         1585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3368832,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001569 DATLJT010000000
VERSION     DATLJT010001569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368832"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VM910_35935"
     CDS             complement(<1..1569)
                     /locus_tag="VM910_35935"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HVI67932.1"
                     /translation="MTQVEHSPMANAIRALAMDAVEKAKSGHPGLPMGAADIATVLFT
                     QFLKYDADHPDWPDRDRFVVSAGHGSMLLYALLYLTGNPDMTLDQIKHFRQLGSLTPG
                     HPENFHTKGVETTTGPLGQGIATSIGMAVAEKMLAAEFGKKIVDHHTYVLVSDGDLME
                     GVSQEAIALAGHWRLNKLIVLYDDNGISIDGPTSLADSVDQVKRFKSAGWAAELIDGQ
                     DQQAIAAALTRAQKSNKPSLIACKTTIGYGAPHKAGTAKAHGEALGADELKAAKENLG
                     ISLEPFSVPDDVLKSWREAGSRGAAARKAWEARLAEMGPRKRAEFERRLRHERPASLA
                     KALKEHKKALLVSPLNVATRKSSEAAIDAIAAAMPMEFLAGSADLTGSNNNKAKSAVS
                     FSAKTPKGRFIHYGVREHGMAAALNGIFLHGGFAPNGATFLIFSDYARPAMRLAALMG
                     AGVNYVMTHDSIGLGEDGPTHQPVEHLSALRAIPNMRVFRPCDAIEVAECWELALNRI
                     NGPTVLALTRQNVPQ"
BASE COUNT          249 a          507 c          533 g          296 t
ORIGIN      
        1 ttgcggcacg ttctggcggg tcagcgccag caccgtcgga ccgttgatgc ggttgagcgc
       61 gagttcccag cattcggcga cctcgatcgc gtcgcacggg cggaacacgc gcatgttggg
      121 aatggcgcgc agcgccgaaa gatgttcgac cggctgatgc gtcggcccgt cctcgccgag
      181 tccgatcgag tcgtgggtca tcacgtagtt gacgccagcg cccatcagcg cggcgagccg
      241 catggcgggg cgggcgtaat cggagaagat caggaacgtc gcgccgttcg gcgcaaagcc
      301 gccatgcagg aagatgccgt tcagcgcggc cgccatgccg tgctcgcgaa cgccgtagtg
      361 gatgaaacgg cctttgggcg tcttggccga gaacgacacc gccgacttcg ccttgttgtt
      421 gttggagccg gtcaggtcgg ccgagcctgc caagaactcc atcggcatcg cggctgcgat
      481 cgcgtcgatc gcggcttcgg aggatttgcg ggtcgcgaca ttgaggggcg agacgagcag
      541 cgccttcttg tgctccttca gcgcttttgc cagcgacgcc ggacgctcgt ggcgcaggcg
      601 gcgttcgaac tcggcgcgct tgcgcggacc catctccgcc aatcgcgctt cccacgcctt
      661 gcgcgccgcc gcgccgcggc tgccggcctc gcgccagctc ttcaggacat cgtcgggcac
      721 cgagaacggc tcgagcgaga tccccagatt ttccttcgcc gctttcaatt cgtcggcgcc
      781 gagcgcttcg ccatgcgcct tcgcagtgcc ggccttgtgc ggcgcgccgt agccgatcgt
      841 ggtcttgcag gcgatcagcg acggcttgtt ggacttctgc gcgcgcgtca gcgccgccgc
      901 gatggcctgc tgatcctgcc cgtcgatgag ctccgccgcc cagccggccg acttgaaacg
      961 cttcacctgg tcgaccgaat cggcaagcga cgtcggcccg tcgatcgaaa tgccgttgtc
     1021 gtcgtagagc acgatcagct tgttgagccg ccagtggccg gcgagcgcga tggcttcctg
     1081 cgacacgcct tccatcaaat cgccatcgga gacgaggacg taggtgtgat ggtcgacgat
     1141 cttcttgccg aactcggcgg cgagcatctt ttccgcaacc gccatgccga tcgaggtcgc
     1201 gatgccctgg ccgagcgggc ctgtggtcgt ctcgacgccc ttggtgtgga agttctcggg
     1261 atgccccggc gtcagcgatc cgagttgacg gaagtgcttg atctggtcga gcgtcatgtc
     1321 cggattgccg gtgaggtaca gcagcgcata gagcagcatc gagccgtggc cggccgagac
     1381 aacgaatcgg tcacggtccg gccagtccgg atgatcggcg tcgtacttga gaaattgcgt
     1441 gaagagcact gtcgcgatat cggccgcgcc catcggcagg ccggggtggc cggacttcgc
     1501 cttctcgacg gcgtccatcg cgagcgcacg aatggcgttg gccatagggg aatgttcgac
     1561 ctgcgtcatg atgaaaaatc cgctt
//
LOCUS       DATLJT010001570         2252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_839430,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001570 DATLJT010000000
VERSION     DATLJT010001570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839430"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VM910_35940"
     CDS             <1..447
                     /locus_tag="VM910_35940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686915.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HVI67933.1"
                     /translation="LYRQPNIAAVSDAIIQIRKGSMGTLVPTSLDAPFKLLRVLEAGG
                     HVAMIVDQHYVNGVDVTFFGRRCKANPFLARLARHVDCPIHGTRIIRMPDRHRFRVDL
                     TEAIEPPRDADGKIDIEGAMQAITSVVEGWVREHPEQWLWVHRRWR"
     gene            complement(472..1572)
                     /locus_tag="VM910_35945"
     CDS             complement(472..1572)
                     /locus_tag="VM910_35945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI67934.1"
                     /translation="MRRAIRFALVAAATMAIAPAAAQPKQRVVNVYNWSDYMAPGVVE
                     DFSKETGIKVRYDTFDSNDTLETKLLAGKSGYDVVVPTAYFLERQIKAGVFQKLDKAR
                     LPNLANMWPEISQRLAAYDPGNQYAVNYMWGTTGIGYNVRKTRELLGANGKIDSWDIV
                     FKPENLAKFKDCGVHLLDSSDDILAAALHYLGLDPNTTNEADLQKAADLVSKIRPYVR
                     KFHSSEYLNALASGEICLVVGFSGDIKQSQKRAVEARNGVEIAYAIPKEGAQLWFDNL
                     AIPRDARNAAEADAFINYLQKPEVAAKNTNLISYANGNLASQKFIDKSILDDKTIYPD
                     GATMRELYTISAHDPKTQRLMNRLWTRIKTGK"
     gene            complement(1665..2000)
                     /locus_tag="VM910_35950"
     CDS             complement(1665..2000)
                     /locus_tag="VM910_35950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI67935.1"
                     /translation="MSAQLGRENVHDLAPEEVARGIAEGKVLLVDVREPNETEVERYP
                     QAFYLPMSQFDPAAIPDPQGRRVVFACRSGNRSVTASLMAQAAGLPYDAHLAGGIKAW
                     KEVGLPTES"
BASE COUNT          402 a          700 c          684 g          466 t
ORIGIN      
        1 ctgtaccgcc agcccaatat cgccgccgtg agcgatgcga tcatccagat ccgcaaaggc
       61 agcatgggca ccctcgttcc gaccagcctc gacgcgccgt tcaagctgct gcgcgtgctc
      121 gaagccggcg ggcacgtcgc catgatcgtc gaccagcact acgtgaatgg cgtggacgtg
      181 accttcttcg ggcgtcgctg caaagcgaac cccttcctcg cccgcctggc gcggcacgtc
      241 gattgcccga ttcatggtac gcgcatcatc cgcatgcccg accgccaccg ctttcgcgtc
      301 gatctgaccg aagccatcga gccgccgcgc gatgccgacg gcaagatcga catcgagggc
      361 gccatgcaag cgatcacatc ggtggtcgaa ggctgggtgc gcgagcaccc cgagcaatgg
      421 ctgtgggtgc atcggcgctg gcggtgataa gaacgtcgcc gcgcctcgct attatttgcc
      481 ggtcttgatc ctggtccaca gccggttcat cagccgctgc gtcttcggat cgtgggcgct
      541 gatggtgtaa agctcccgca tcgtcgcgcc gtccggatag atggtcttgt cgtcgaggat
      601 cgatttgtcg atgaatttct ggctcgcgag atttccgttg gcataggaaa tgaggttcgt
      661 gttcttcgcg gccacctcgg gcttctgcag ataattgatg aacgcgtccg cttcggcggc
      721 gttcctggca tcgcgcggga tggcaaggtt atcgaaccac aattgcgcgc cttccttcgg
      781 tatggcgtag gcgatctcga ccccgttcct ggcttccacg gcacgctttt gcgattgctt
      841 gatgtcgccg gaaaagccca ccaccagaca gatctcgccg gaggcgagcg cgttgaggta
      901 ttccgatgag tggaacttgc gcacgtaggg cctgatcttg ctcacgagat cggcggcctt
      961 ttgcagatcg gcctcgttgg tggtgttggg gtcgagccca agataatgca gcgctgccgc
     1021 caggatgtcg tcggaggaat cgagcagatg cacgccacag tccttgaact tggccaagtt
     1081 ctcgggcttg aagacgatat cccagctgtc gatcttgccg ttcgccccca gcagctcacg
     1141 cgtctttctg acgttgtagc cgatgccggt cgtgccccac atgtaattca cggcgtactg
     1201 gttgccggga tcgtacgccg ccaatcgctg tgagatctcc ggccacatgt tggcgaggtt
     1261 cggcagcctc gccttgtcca gcttctggaa aacaccggct ttgatctggc gctcgagaaa
     1321 ataggccgtc ggcaccacca cgtcgtagcc ggatttgccg gccagaagct tcgtctcgag
     1381 cgtgtcgttg gaatcgaatg tgtcgtagcg gaccttgatg ccggtctcct tgctgaaatc
     1441 ctcgacgacg cccggagcca tgtagtcgga ccaattgtag acgttgacga cgcgctgttt
     1501 cggctgcgcg gcagcaggcg cgatcgccat ggtggcggct gcaacgaggg caaaccggat
     1561 tgctcggcgc atggacgggc tccttcagga ccatcggacg aaaagatcgg ggaaagacgt
     1621 gctcgggaga tggcggaccg gctcgcgcgt ctccgcagcg cgcttcagct ctcggtcggc
     1681 aaaccgacct ccttccacgc ttttatcccc ccggcgagat gggcatcgta gggtagcccc
     1741 gccgcctgcg ccatcagcga cgcggtgacc gaccgattgc ccgagcggca agcgaacacg
     1801 acccggcggc cctggggatc gggaattgcg gccggatcga actgcgacat cgggagatag
     1861 aaggcttgcg gatagcgctc gacttcagtc tcgttcggct cgcggacatc caccagcaga
     1921 accttgccct cggcgatccc ccgcgccacc tcttccggcg ccaaatcgtg cacgttctct
     1981 cggccaagct gagcggacat gaggtctact ccttaattca ggggcggcgt cgccgctgct
     2041 gaaaacaagt cgcgcgtcac actgcaagaa tcaagacccc ggcgattgcg gtgccggcta
     2101 gtgtcccaat tccgaagttc gcatcatttc gccgcggcct cgtttgcgaa ttttggaatc
     2161 gaaggacact agcaacttat tgatctagtg tcccgattcc gaagttcgca tcatttcgtc
     2221 gcggcctcgt ttgcgaactt cggaatcgag cc
//
LOCUS       DATLJT010001571         5691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3600982,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001571 DATLJT010000000
VERSION     DATLJT010001571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600982"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VM910_35955"
     CDS             <1..628
                     /locus_tag="VM910_35955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI67936.1"
                     /translation="IALMLFGTGLAFYLGKPLIEPTAPRLPAIDFGWWSDIPQVQAAL
                     RINVMFLIGVALAPTLYWAFRTTRWGLLIRTAGESSDAARAMGHSVLLIRLRATMVGG
                     FLAGIGGSFLSLFYPGSWNEGLSSGQGITAVALVIFARWDPMLCLWASLAFGGAAALG
                     PALQSVGVTSGYHLFNAAPYILTLAIMIITCSPKRTLTGAPAELSITR"
     gene            643..1326
                     /locus_tag="VM910_35960"
     CDS             643..1326
                     /locus_tag="VM910_35960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVI67937.1"
                     /translation="MPDRYIKSEPYAWPYNGDLRPQNTALIIIDMQTDFCGGGGYVDK
                     MGYDLSHTRAPIEPIKRVLKVMREKGYHVIHTREGHRPDLADLPANKRWRSRQIGAGI
                     GDPGPCGRILVRGEAGWEIIPDLSPLPGEPVIDKPGKGSFCATDLELMLRLRGIENIV
                     LTGITTDVCVHTTMREANDRGFECVLIEDCCGATDKSNHDHALKMIKMQGGVFGAVAK
                     SADLIGAIS"
     gene            1323..2855
                     /locus_tag="VM910_35965"
     CDS             1323..2855
                     /locus_tag="VM910_35965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI67938.1"
                     /translation="MIVGEAQPPKGAFGVDAIAMTMRFGDFLALDNVELKVRPGTFHA
                     LLGENGAGKSTLVKCIMGYYHPTEGDVLVGGREQAISNPKDAHALGLGMVYQHFTLVP
                     AMTVAENLVLARDDVPAVVDWAKEKKELARFLSRMPFKVPLDARVSDVSAGERQKCEI
                     LKQLYLKRRFLILDEPTSVLTPGEADEVLGMLRAMVVAGDLTILMITHKFREVMAFAD
                     EVTILRRGKLAGKGLVKDLTPDAMARTMIGAEELTIQPARTGEAGEPRLELEKVRALD
                     DAGAIAVRDVSLTVRGGEIVGIAGVSGNGQRQLVEVLAGQREAESGEIRVAGSAYHAR
                     REEMRSHKMSLLPEEPLKNACVGGMSVADNIAFREFDRAPFASGGWWLNRGAFRDDAR
                     RKIARYKIKTRTPDTPISALSGGNVQRAVLARELGGDVEVLIAANPCFGLDFAAVAQI
                     HAEIMAARNRGAAVLLVSEDLDELLELSDRLVVMFHGQFVYEARASEANLTEIGRHMA
                     GH"
     gene            2855..3355
                     /locus_tag="VM910_35970"
     CDS             2855..3355
                     /locus_tag="VM910_35970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HVI67939.1"
                     /translation="MSDTAGLGRVEFDKLFLRRSFEVARRSMTHGNHPFGAILVDQGG
                     KVLLEAENGYMPAQDGTAHAERLLATEACRTLNADVRAKATLYSSAEPCAMCAGAIYW
                     AGIGRVVYGLSEHRLRDITGNHPENPTLDLPCREVFKSGQRPTEIVGPMLEDEAAALH
                     EGIWTK"
     gene            complement(3441..4580)
                     /locus_tag="VM910_35975"
     CDS             complement(3441..4580)
                     /locus_tag="VM910_35975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67940.1"
                     /translation="MSIRKTAIAAAALAFAALASTGLARAADLPVKAQKPPPDLPFFL
                     LIDDRVTFSWMPKGTDPGVFSTNPNGSLNSTTAKQVYSFTHFDIWQYGTNFFTISMFK
                     SDQNDPANPCTNSGVITSPLAGFATVPATCEGATEVYGLFRSTFSWNDIFGTKAFTMG
                     PLHNISFEVGMDANTENNYLSPAKRDVVAGLQFQFDLPYKGFFNVAPLVYWEFANRNA
                     FTQCGAGWQLPAPAVPGVNCLVDGNRSFNPTWAVETNYYMDLGFLPPGFDFFSISGRA
                     AWYGKKGTENNPLPFNPFPVAAPFNSNTAVEFNSEPIRLTFDASKAMWGPKYTHFVDV
                     WVAYRYWQNKFGLDHKVSAVCNISPGISNNTCTEQTVYTGITVKF"
     gene            5159..>5691
                     /locus_tag="VM910_35980"
     CDS             5159..>5691
                     /locus_tag="VM910_35980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI67941.1"
                     /translation="MSFSTPSRQLPPTTPLLQTIGLTKRYGTFLANDSIDIDIWPQQI
                     HALLGENGAGKSTLVKAIYGLIQPSEGEMRWQGQPMVLSGPSEARARGIGMVFQHFSL
                     FENLTVAENVALGLDGKESFRDMSARLERVSRVYGLPLDPRREVWQLSVGERQRIEIV
                     RALMQDPKFLILDEPTAV"
BASE COUNT         1089 a         1735 c         1802 g         1065 t
ORIGIN      
        1 catcgcactg atgctgttcg gcaccggtct cgccttctat ctcggcaagc cattgatcga
       61 gccgacggcg ccgcggctgc ccgcgatcga tttcggctgg tggagcgaca tcccgcaggt
      121 ccaggcggcg ctgcgcatca acgtgatgtt tttgatcggc gtggcgctcg cgcccacctt
      181 gtattgggcg tttcggacaa cgcggtgggg ccttttgatc cgcaccgccg gcgaaagctc
      241 ggatgctgca cgcgcgatgg gtcattcggt gctgctgatc cgcctgcgcg cgaccatggt
      301 cggcggtttt cttgcgggga tcggcggatc gttcctgtcg ctattctatc cgggtagctg
      361 gaacgagggc ttgtcaagcg gacaaggcat caccgcggtc gcgctcgtca tcttcgcgcg
      421 ctgggacccg atgttgtgcc tgtgggcctc gcttgccttc ggaggcgccg ccgcactcgg
      481 accggcgctg cagtcggtcg gcgtcacctc cggctaccac cttttcaacg ccgcgcctta
      541 catcctgact ttggcgatca tgatcatcac ctgctcgccc aagcgcacgc tgaccggcgc
      601 gccggccgag ctttcgatca cccgatagtc aagagacccg acatgcccga tcgttacatc
      661 aagtccgagc cctacgcctg gccctataac ggcgacctgc gcccgcaaaa caccgcgttg
      721 atcatcatcg acatgcagac cgatttctgc gggggcggcg gctacgtcga caagatgggc
      781 tacgatctgt cgcacacgcg cgccccgatc gagccgatca agcgcgtgct caaggtcatg
      841 cgcgagaagg gctaccacgt catccacacc cgcgaagggc accgtcccga cctcgccgac
      901 ctgcccgcca acaagcgctg gcgctcgcga cagatcggcg ccggcatcgg cgaccccggc
      961 ccgtgcggcc gcatcctggt gcgcggcgag gccggctggg agatcattcc cgatcttagc
     1021 ccccttcccg gcgagccggt catcgacaag cccggcaaag gctcgttctg tgccaccgac
     1081 ctggagctga tgctgcggtt gcgcggcatc gagaacatcg tgctgaccgg catcaccacc
     1141 gacgtctgcg tccataccac catgcgcgaa gccaacgatc gcgggttcga atgcgtcctg
     1201 atcgaggatt gctgcggggc caccgacaag agcaaccacg accacgcgct gaagatgatc
     1261 aagatgcagg gcggcgtatt cggcgcggtc gcaaaatcgg cggacctgat cggagcgatt
     1321 tcatgatcgt cggcgaggcg cagccgccga aaggcgcctt tggcgtcgac gccatcgcca
     1381 tgactatgcg cttcggcgat tttcttgcgc tcgacaatgt cgagctcaag gtgcgtccag
     1441 ggacgttcca tgccctgctc ggcgagaacg gtgcggggaa gagcacgctc gtcaaatgca
     1501 tcatgggcta ttaccacccg accgaaggcg atgtcctggt cggcggccgc gaacaagcca
     1561 tctccaaccc caaggacgcc catgcgctcg ggctcggcat ggtctaccag cacttcaccc
     1621 tggtgccggc aatgacggtg gccgaaaacc tggtgctggc gcgtgacgat gttcctgccg
     1681 tggtcgactg ggccaaagaa aagaaggaac tcgcgaggtt tctttcgcgg atgccgttca
     1741 aggtaccgct cgatgcccgc gtgtcggatg tttccgcggg cgagcggcag aaatgcgaga
     1801 tcctgaagca gctctatctc aaacgacgct ttctcattct cgacgagccg acttcggtgc
     1861 tgacaccggg agaggccgac gaagttctgg gaatgctgcg cgcgatggtg gtggccggcg
     1921 atctcacgat cctgatgatc acccacaaat tccgcgaggt gatggccttt gccgacgagg
     1981 tcaccatcct tcggcgcggc aagctcgcgg gaaaaggcct ggtcaaggac ctaacccccg
     2041 atgcgatggc ccgcaccatg atcggcgcgg aggaattgac catccagccg gcacgcaccg
     2101 gcgaggctgg cgagccgcgg ctcgagctcg aaaaggtccg cgccctcgac gatgccggtg
     2161 cgatcgcggt ccgcgacgtg tcgctcaccg tccgtggcgg ggagatcgtg ggcatagccg
     2221 gcgtctccgg caacggccag cggcagctcg tcgaggtgct cgccggccaa cgcgaagcgg
     2281 aaagcggcga gatccgcgta gcaggcagcg cctatcatgc aaggcgcgaa gagatgcgca
     2341 gccataagat gtcgctgctg ccggaggagc cgttgaagaa tgcctgcgtc ggcggcatga
     2401 gcgtggccga caatattgcc tttcgcgagt tcgaccgcgc gccgtttgcc agcggcggct
     2461 ggtggctcaa ccgcggtgca ttccgcgacg acgcaaggcg caagatcgcc cgctacaaga
     2521 tcaagacgcg cacgcccgat acgccgattt cggcgctgtc gggcggcaac gtgcagcgcg
     2581 cggtgctggc gcgcgagctt ggcggcgatg tcgaggtgct gatcgccgcc aatccgtgct
     2641 ttgggctgga tttcgcggcc gtcgcgcaga tccatgccga gatcatggct gcgcgaaatc
     2701 gcggcgccgc cgtgctcctg gtcagcgaag atctggacga actcctggaa ttgtcggatc
     2761 gtctggtcgt gatgttccac ggccagttcg tctatgaggc gcgcgcgagc gaagccaatc
     2821 tgaccgagat cggccggcat atggcagggc attgatgagc gatactgccg gcctcgggcg
     2881 cgtcgagttc gacaagcttt ttctgcgccg ctccttcgag gtggcgcgcc gctcgatgac
     2941 ccacggcaac catcccttcg gcgccatcct ggtcgaccaa ggcggcaagg tgctgctgga
     3001 agccgaaaac ggttacatgc cggcgcagga cggcacggcg catgccgagc ggttgctcgc
     3061 gaccgaagct tgccggacct taaacgccga cgtgcgcgcc aaagctacgc tctattcgtc
     3121 cgccgaaccg tgtgcgatgt gcgccggcgc aatctattgg gccggcatcg gccgcgtggt
     3181 ttacggcctg agcgagcatc ggctgcgcga catcaccggc aatcacccgg agaatccgac
     3241 gctcgacctg ccctgccgtg aggtcttcaa aagcggccag cgaccgaccg agatcgtggg
     3301 cccgatgctg gaggacgagg cggcagcgct gcacgaaggc atctggacga aatagcggcc
     3361 ggcaattttt ccgcaaatga aaatggcgcc gcgggtacat cgcggcgcca ttctttttca
     3421 cctggaagtt aatcaggcga tcagaacttc acggtgatgc cggtgtagac cgtttgctcg
     3481 gtgcaggtat tgttgctgat cccaggcgag atgttgcaga ccgcgctcac cttgtgatcc
     3541 agaccgaact tgttctgcca gtagcggtag gccacccaga cgtccacgaa atgggtgtac
     3601 ttcggacccc acattgcctt ggaggcatcg aaggtcagac ggatcggctc agagttgaat
     3661 tcaaccgcag tgttgctgtt gaagggcgcg gcgacgggaa atgggttgaa cggaagcggg
     3721 ttattctcgg ttcccttctt gccgtaccag gcggcgcggc cgctgatcga gaagaagtcg
     3781 aagcccggcg gcaggaagcc gagatccatg tagtaattgg tttcgacggc ccaggtcggg
     3841 ttgaagctgc ggttgccgtc gaccagacag ttcacgccag gaactgccgg cgcgggcaat
     3901 tgccagccgg cgccgcactg ggtgaaggcg ttgcggttgg caaactccca gtacaccaat
     3961 ggcgcgacgt tgaagaagcc cttgtagggc aggtcgaact ggaactgcag accggcgacg
     4021 acgtcgcgtt tcgcgggcga gaggtagtta ttctccgtgt tggcgtccat gccgacttcg
     4081 aacgagatgt tgtgcagcgg ccccatcgtg aaagccttgg tgccgaaaat atcgttccag
     4141 ctaaatgtcg aacggaacag accgtagact tcggtcgccc cttcgcaggt cgccggaacg
     4201 gtggcgaagc cggccaacgg agaggtgatc acgccggaat tggtgcaggg gttggccgga
     4261 tcgttctgat ccgacttgaa catcgagatc gtgaagaagt tggtaccgta ctgccagata
     4321 tcgaaatggg tgaacgagta gacctgcttg gcggtcgtgc tgttgagcga accgttcgga
     4381 ttggtcgaga agacaccggg gtcggtacct ttcggcatcc acgagaaggt cacccgatca
     4441 tcaatcaaga ggaagaacgg aagatccggc ggcggcttct gggccttgac cggaaggtcg
     4501 gcggctcttg caagcccagt tgatgctagt gcggcaaatg caagcgccgc ggctgcaatt
     4561 gctgttttgc ggatagacat ttcaaaactc ccccaagttg acgaacgaac gatctaaggc
     4621 ccttgcagac gagattgcgc ggttcatcgg tggagagaag cctcatcttt ccacaggagg
     4681 ttgctgcaat ttttcgcaaa agacggagat cgcataccga gatgagcagc agcgacgatg
     4741 tttgctgcgc tgcctgcaat ccgcggcgac cgaaaatgct tctgtttaat ctctgttttt
     4801 cttgtgcttt tcgacggcct gcgcagcgcg cccacaattc aaatcggggc aagcgttcgg
     4861 tctacgcctc agccgcgaag ctactgataa cgctgcttaa cttcgcggca gggcagagaa
     4921 tggaactccg aattgttgcg atcgtcacaa ttttgcaaca gcttgtagcc gtccggtccg
     4981 cccctgccgc agcgttccga aagctccggc cggaacgatc ggtacgaatc gtacgaaaca
     5041 agcgggattt cgccaatgcc gaaaggttgg gcaagagctg gcgctttttc gctacttacg
     5101 cagacgtcta aagtggccca ctattgaggc atcccctctc gcctgcgtga cgctcagaat
     5161 gtccttttcg acgcccagca gacagcttcc tcccacaacg ccgctcctgc aaaccattgg
     5221 actgacaaag cgttacggca cttttctcgc caacgattcc atcgatatcg atatctggcc
     5281 gcagcaaatc cacgcgctgc tcggcgagaa cggcgcggga aaatcgaccc tggtcaaggc
     5341 catctacgga ttgatccagc cgagcgaggg ggagatgcgc tggcagggac agccgatggt
     5401 cttgtctgga ccatcggaag cgcgcgcgcg cggcatcggc atggtgttcc agcatttctc
     5461 gctgttcgag aacctcacgg tcgccgagaa cgtcgcgctc gggctggacg gcaaggagtc
     5521 cttcagggac atgtccgcgc gcctcgaacg agtctcgcgt gtctacggac tcccgctcga
     5581 tccgcggcgc gaggtgtggc aactgtcggt cggcgagcgc cagcgcatcg agatcgtccg
     5641 cgcgctgatg caggatccga aatttctgat cctcgacgag ccgaccgccg t
//
LOCUS       DATLJT010001572         3197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_382174,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001572 DATLJT010000000
VERSION     DATLJT010001572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382174"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..322
                     /locus_tag="VM910_35985"
     CDS             146..322
                     /locus_tag="VM910_35985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HVI67942.1"
                     /translation="MMWKTTFEPPEGRLPLYSLQPAGLVMAVKSAAAEREMSQAAVVR
                     EALMSYLAAIKEGH"
     gene            326..679
                     /locus_tag="VM910_35990"
     CDS             326..679
                     /locus_tag="VM910_35990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67943.1"
                     /translation="MPRFEPGNRLGGRPRGARNKLAKRFFEDMSEVWDEPTADGKSTR
                     GKAALRLMWRERPGEFAKLYAAVMPREFWVDNVAGELTDDEIDRAIEMLREQIRGERA
                     LELKADELKMIEYDQ"
     gene            669..1355
                     /locus_tag="VM910_35995"
     CDS             669..1355
                     /locus_tag="VM910_35995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67944.1"
                     /translation="MISDSEKQKLQAALEQLEIERQRRIEAKIESGEAVLRRDIAVVG
                     VARDNGGDGPIERDAEGREIYYLVGGPDGRGTYCTRPMTIVTGVPRSGRDTPDPDPPV
                     KFARQPSPTRKIRADNKAKRYAHPRAIRSAATGAAPAPEPVVREPKEIWIQLQAPDER
                     NLAGVIARGVYRHPDSLVRVYDTDGNLLGSAPLRPGDSAEVAAKRLLREKGAAGNFYR
                     PIRYPRGSVH"
     gene            2301..2708
                     /locus_tag="VM910_36000"
     CDS             2301..2708
                     /locus_tag="VM910_36000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVI67945.1"
                     /translation="MPRTAISGLVFTTFLVLLVFPAVPQEPAAKGADTESGQQTFNNA
                     CRTCHTTKEGDNRLGPNLHNIIGRKAGSLQNYGYSSAMKGADFVWDKDKLDRFIAKPD
                     EVVPGNNMKPYGGLTSAEDRAKVIAFLESPTTN"
     gene            2877..>3197
                     /locus_tag="VM910_36005"
     CDS             2877..>3197
                     /locus_tag="VM910_36005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67946.1"
                     /translation="MKVQTRREAVILMTAISLLSTDAVAQPDRLDALFNQPAENLSVP
                     SRVRQRYAILAYYIQLLNLQGPMRSRSEDIAAAIKTYQLVGESIARSDARFEGEVQAS
                     FVQSL"
BASE COUNT          704 a          910 c          978 g          605 t
ORIGIN      
        1 cgcggcgtcc tgcggacgga ttaccgtcca ttcggcgcct ctgctcaacg cgggcgtcct
       61 cgcaagccgg acaagctatc gaatgcggag aagcagcgcc gctaccggca aaggctgcgc
      121 gctaagcgaa tgcaggaaaa ctgagatgat gtggaagacg acgtttgagc cgcccgaggg
      181 ccgattgccg ctctattcgc tacagccggc gggtctggtt atggccgtca agtcggcggc
      241 tgccgaacgg gaaatgagcc aggccgctgt agtgcgggaa gcgctgatga gctacctcgc
      301 tgcgatcaaa gaggggcact agccaatgcc gcgctttgaa cccggcaacc gtcttggcgg
      361 ccgcccgcgc ggcgcccgca acaagctcgc aaaacgcttc ttcgaggaca tgtccgaggt
      421 gtgggacgag cccactgccg atggcaagtc aacgcgcggc aaggcagcct tgcgccttat
      481 gtggcgcgag cggccgggtg agtttgcgaa gctctacgcc gcggtcatgc cccgcgagtt
      541 ttgggtcgac aatgtcgccg gtgaattgac cgacgacgag atcgaccgcg cgatcgaaat
      601 gctgcgagag caaatccgcg gggaacgcgc gctcgaatta aaggccgacg aactcaagat
      661 gatcgaatat gatcagtgat tccgaaaagc agaagctcca ggcggcgctt gagcagctag
      721 agattgagcg gcagcgtcgg atcgaagcga agatcgagag cggtgaggct gtcctgcgtc
      781 gtgatatcgc cgtggtgggc gttgcccgcg ataacggtgg ggacgggccg atcgagcgag
      841 atgccgaggg gcgtgagatt tattacctcg tgggaggccc ggatggacgc gggacttatt
      901 gcacgaggcc aatgacgatt gttaccggcg tcccaagaag cgggcgggat actcccgacc
      961 ctgatcctcc cgtcaagttc gcaaggcaac caagtccgac acggaaaatc cgggcggaca
     1021 ataaggcgaa gcgctacgcc cacccgaggg cgatccgctc cgcggcgacc ggagccgcgc
     1081 cggcgccgga gccggtcgtg cgtgagccga aggaaatatg gattcagctt caggcacccg
     1141 atgagcgcaa tctcgctggt gtgattgcgc ggggtgtgta caggcacccc gacagtttgg
     1201 ttcgcgtcta tgacacggac ggcaacctgc taggcagtgc gcctttgcga ccgggagact
     1261 ctgccgaggt tgcggctaag cggctactcc gcgagaaggg cgccgcgggc aatttttaca
     1321 ggccaattcg gtatccccgc ggttcggtgc attaacgaaa agggccgctg ctgcggaaac
     1381 tgctccgaga gaggaaggta tgtaccagcg atattttggg cgttcgcgtc gctccagctc
     1441 atgacgagcc tcgcagcgag ccgaacaata gcgaacgtgg atccaccagc ggccattcca
     1501 caaattacgg gagcggacac ctaatccgag ttttccgtga cattgggcgc atcttttcat
     1561 tgctggtcct tctcagccac ccgtcgcctg ttggtcaccc gctgagagta agggttcaaa
     1621 tgcggaagac gaaatatggt ttcgtggaag gcaaaaaagg tttcaattgt gtttcgtgcg
     1681 gaactcgccc gggtggaaga gccggggcgt gccattcgcc aaaacgagaa ccccgcccga
     1741 ttcccaaggg cggggtctcg ggaaccctca tgcacgggct tggcgatacg gggccttttc
     1801 aagctagcaa aaaaggagct aaatttcaaa aatctaaatt tatagggagg ttgaccatgt
     1861 cgagttcaaa gttgccggtt tggcttatcg ctacgatgat gcttcttccg atgggcttcg
     1921 ttctttatcc tcagggctct gcaaatgctg ccaagtactg tgcgcagtta agaggggcaa
     1981 cagccgaagg acatcctgac tgctcatttt cgacacttga taagtgtcgc gcgcgcgtaa
     2041 ggcacagggg cggcggccac tgctacagat cgcactagat ctcccgagca aaattagcat
     2101 ttagagaagt cgtgacgccc gcgcggtgga gcgctggcct tgtcagacta cgcgcaggcg
     2161 ccggctgtcc tcgatcactc gcgcaccttg ccgcaaggtg atgggcgtgc cgggccaggt
     2221 gcccacaccg tggcaagaca tctcgccggg atcactggaa cgaatcgttg cgactgcgcg
     2281 ttcaatcgcc aggagcactt atgccccgca ccgcaatctc gggattggtc ttcaccacat
     2341 tcctagtgct tctggttttc ccggccgtgc cccaggagcc ggcggctaag ggcgccgaca
     2401 ccgagtcggg gcaacaaaca ttcaacaacg catgccggac ctgccacacg accaaggaag
     2461 gcgacaatcg cttgggtccg aatctccaca atattatcgg aaggaaagcc ggttcgctcc
     2521 agaactacgg ctattccagc gccatgaagg gcgcggactt tgtatgggac aaggataagc
     2581 tcgatcgctt catcgcgaaa cccgatgagg ttgtgcccgg caacaacatg aagccgtatg
     2641 gcggcctcac atcggccgag gaccgtgcca aggtgattgc gttcttggag tcgcccacta
     2701 ccaattgagc ggttacgtca cgttcccgat ccctgaaaag gccgcgatct gctaacctct
     2761 cccctaatcc cgggcgcgcg ccgtgtcgtt ctgcggcata gacaatcggc ccgagctgta
     2821 gataaaattt cagcgcacgg gacactccac atggctgacc ccagcacttc aggagcatga
     2881 aagtgcagac gcgacgagaa gccgtgatac tgatgactgc tatttcgctg ctatcaaccg
     2941 acgcggtcgc ccagccagac aggctggacg ctcttttcaa tcagccggct gagaaccttt
     3001 cagttccatc gcgcgtccgc cagcgttatg caatcctggc ttattacatt caactgctga
     3061 acctccaggg accgatgcgt tcgagatctg aagatattgc cgctgcaata aaaacttatc
     3121 aattagtcgg cgagagcatt gccaggagcg atgcaagatt tgagggagaa gtgcaagcat
     3181 cgttcgtcca gagccta
//
LOCUS       DATLJT010001573         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2221110,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001573 DATLJT010000000
VERSION     DATLJT010001573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221110"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VM910_36010"
     CDS             <1..1111
                     /locus_tag="VM910_36010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVI67947.1"
                     /translation="MRLILEMDGEIIERADPHIGLLHRGTEKLIEHKTYLQAIPYFDR
                     LDYVSPMCQEHAFALAVEKLIGITPPPRAQYIRVAFAEITRILNHLLCICAFGLDVGA
                     ITPFLWGFEERERLMEFYERVSGARMHANYFRPGGVHLDLPAGLADDILAFCDTFPQT
                     IDDIERLLTDNRIFKERTVDIGVVTAEQAADWGFSGPMLRGSNVAWDLRRAQPYDAYD
                     KIEFDVPVGRNGDSYDRYLVRIEEMRQSVRIIKQAIAQMPAGPVKVDDRKMAPPPRAE
                     MKRSMEALIHHFKLYTEGYHVPAGETYSAVEAPKGEFGVYLVSDGTNRPYRCKIRSPG
                     FAFLQATDFMTRGHMLADVVAIIGSMDIVFGEVDR"
     gene            1108..1710
                     /gene="nuoE"
                     /locus_tag="VM910_36015"
     CDS             1108..1710
                     /gene="nuoE"
                     /locus_tag="VM910_36015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HVI67948.1"
                     /translation="MSAPVDAAEIDQPADFDFTPENLEQAKAHIAKYPPGRQASAVLP
                     LLDLAQRQHAGWLPRAAMDRVAEMLGMAPIRVYEVATFYTMLNLRPVGRYLLQACTTT
                     PCWLRGSDEVVHACEKKLGVGIGGTTPDGMFTLVEVECLGACVNAPILQVNDDFYEDL
                     DGPATEALLDSLREGKVRPPGSVAGRHGSEPVGGRTTLLE"
     gene            1721..3013
                     /gene="nuoF"
                     /locus_tag="VM910_36020"
     CDS             1721..3013
                     /gene="nuoF"
                     /locus_tag="VM910_36020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HVI67949.1"
                     /translation="MLADGDRIFTNLYGRSDWRLAGAQRRGDWDGTKELILKGRDWIV
                     EQVKESGLRGRGGAGFPTGMKWSFMPKESDRPTYLVVNADESEPGTCKDRDILRNEPH
                     KLVEGCLLASAGMGVHDCYIYIRGEFYNEAQRLQAAIDEAYAAGLIGKDACGSGYRFD
                     LYLHRGAGAYICGEETGLLESLEGRKGQPRLKPPFPAQVGLYGCPSTVNNVETIAVVP
                     EILRRGATWFARIGRPRNTGTKIFCLSGHVNAPCNVEEAMSIPLRELIERHAGGVRGG
                     WDNLLAIIPGGSSVPVIPKSICETVLMDFDSLQAVKSALGTAAVIVMDKSTDIVKAIA
                     RLSKFYKHESCGQCTPCREGLGWVWRVMQRMVEGDASVTEIDMLLDVSYEIEGHTICA
                     LADGAAWPVQGLIRHFRPEMERRIAEYREAAARQRQAA"
     gene            3015..>3437
                     /locus_tag="VM910_36025"
     CDS             3015..>3437
                     /locus_tag="VM910_36025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI67950.1"
                     /translation="MPRLTIDGHEVEVPAGTTIFQACEHARIEVAHFCYHERLAIAGN
                     CRMCLVEVEKSPKPIASCAMPAADGMVVHTNSPKAVKAQKGVMEFLLINHPLDCPICD
                     QGGECDLQDQAMAYGFDRSRFEENKRAVRDNDFGPLVKT"
BASE COUNT          665 a         1023 c         1095 g          654 t
ORIGIN      
        1 gatgcggctc attctggaga tggatggcga gatcatcgag cgcgccgatc cgcatatcgg
       61 tctgctgcac cgcggcacgg aaaagctgat cgaacataag acgtatctgc aggcgatccc
      121 gtatttcgac cggctcgact acgtatcgcc gatgtgccag gagcatgcct tcgcgctcgc
      181 cgtcgaaaag ctgatcggga tcacgccgcc gccccgggcg caatacattc gtgtcgcgtt
      241 cgctgagatc actcggatcc tcaaccacct actctgtatc tgcgctttcg gcctcgatgt
      301 cggagccatc accccgttcc tatggggctt cgaggagcgc gagcggctga tggagtttta
      361 tgagcgcgtc tcgggtgcgc gcatgcatgc caattatttc cgccccggcg gagttcatct
      421 cgatctgcct gccggactcg ctgacgacat cctcgccttc tgcgacacct ttccgcagac
      481 gatagacgac atcgagcgat tgctgaccga taatcgtatc ttcaaggagc gcaccgtcga
      541 tatcggcgtg gtgaccgccg agcaggccgc ggattggggt ttctccggac cgatgctgcg
      601 cggttcgaac gtcgcctggg acctccgtcg agcacaacct tacgacgctt acgacaagat
      661 cgagttcgac gttccggtcg gacgcaacgg ggattcgtac gaccgctacc tcgtccgaat
      721 cgaggaaatg cgccagtcgg tgcgcatcat caagcaggcg atcgcgcaga tgcctgctgg
      781 cccggtgaag gtcgacgacc gcaaaatggc gccgccgccg cgcgccgaaa tgaagcgctc
      841 gatggaagcg ctgatccatc atttcaaatt gtataccgaa ggttaccacg ttccggctgg
      901 cgagacttat tcggcggtcg aggcgccgaa aggcgagttc ggcgtctatc tcgtttccga
      961 cggaaccaac cggccgtacc gttgcaagat cagatcgccg ggctttgcct ttcttcaggc
     1021 caccgatttc atgactcgcg gccatatgct cgccgatgtc gtcgcgatca tcggctcaat
     1081 ggacatcgtc ttcggtgagg tcgaccgatg agcgcaccgg tcgatgcagc cgagatcgat
     1141 cagcccgccg acttcgattt cacccccgag aaccttgagc aggcgaaagc gcacatcgcc
     1201 aaatatcctc ccgggcgcca agcgagcgcg gtgctgccgt tgttggatct ggcacagcgc
     1261 caacatgccg gatggctgcc tcgggcggcg atggatcgcg tcgccgagat gctcgggatg
     1321 gcgccgatcc gcgtctacga ggttgcgacc ttctacacga tgctcaatct gcggccggtc
     1381 ggacgctact tgctccaagc ctgcacgacg acgccgtgct ggctgcgcgg ctcggacgag
     1441 gtggtccacg cctgcgagaa gaagctcggt gtcggcattg gcggcaccac tccggacggt
     1501 atgtttacac tggtcgaagt cgagtgcctg ggtgcctgcg tcaacgcgcc gatcctgcag
     1561 gtcaacgacg atttctacga ggatcttgac gggccggcaa cagaggcgtt gctcgattca
     1621 ttgcgcgagg gaaaggtccg cccgccaggc tcggtagccg ggcggcatgg atccgagcca
     1681 gttggcggtc gcaccacctt gctcgaatga gaggaaggag atgctcgccg acggagatcg
     1741 catctttacc aacctctacg gccggagcga ttggcggctc gccggcgcgc agcgtcgcgg
     1801 tgattgggat ggcaccaagg agctgatcct caagggccgc gattggattg tcgagcaagt
     1861 caaggaatca gggctgcgcg gacgcggcgg cgccggcttt ccgaccggta tgaagtggtc
     1921 tttcatgccc aaggaaagtg accgtccgac ctatctcgtc gtcaatgccg atgagagtga
     1981 gccaggcacc tgcaaagacc gcgatatcct gcgcaacgaa ccgcataagc tcgtcgaggg
     2041 ctgtttgctc gcctcggcgg ggatgggtgt ccacgattgc tacatctaca ttcggggcga
     2101 gttctataat gaggcccagc gtctgcaagc agcgatcgac gaggcttatg ccgcggggct
     2161 gatcggaaaa gacgcctgcg gctccggcta tcgcttcgat ctctacctgc accgcggggc
     2221 aggtgcatac atctgcggcg aggagactgg ccttctcgaa agtctcgaag gacgcaaagg
     2281 tcaaccacgc ctgaaacctc ccttccctgc ccaggtcggg ctctacgggt gcccgagcac
     2341 cgtcaacaat gttgaaacga tcgcggtggt tccggagatc ttgcgccgcg gtgcaacctg
     2401 gtttgcccgg attgggcggc cgcgaaatac cggcaccaag attttctgcc tatccggcca
     2461 tgtaaatgcg ccgtgcaatg tagaagaagc catgagcata ccgcttcgcg agctgatcga
     2521 aaggcatgcc ggtggcgtgc gcggcggctg ggacaatctg ctggcgatca ttcccggcgg
     2581 ttcatcggta ccggttatac cgaaatcgat ctgcgagacg gtgctcatgg atttcgatag
     2641 cttgcaagct gtcaaaagcg cgctcggaac cgcggcggtt atcgtgatgg acaaatcgac
     2701 ggacatcgtg aaggcgatcg cgcggctttc gaagttctac aagcacgaga gctgcggcca
     2761 gtgcacgccc tgccgcgagg gtctaggatg ggtatggcgg gtcatgcagc gcatggtcga
     2821 gggcgacgcc tcggtaacgg aaatcgacat gctgctcgac gtctcctacg aaatcgaggg
     2881 ccacacgatc tgtgccttgg ccgatggggc cgcgtggccg gtgcaggggc tgatccgcca
     2941 cttccgcccg gagatggagc ggcggatcgc ggagtaccgc gaagccgccg cacggcaaag
     3001 gcaggcggcc tgacatgccc aggctgacga tcgacgggca cgaagtcgag gtgccagcgg
     3061 gcaccacgat ttttcaggcc tgcgagcacg cccggatcga ggtcgcgcat ttctgctacc
     3121 acgagcgtct ggcgattgcc gggaattgcc ggatgtgtct ggtcgaggtg gagaaatcgc
     3181 ccaagccgat cgcctcctgt gcgatgcccg cggccgacgg catggttgtc cacacgaact
     3241 cgccgaaggc ggtcaaggca caaaaggggg tgatggagtt cctgctgatc aatcatccgc
     3301 tcgattgccc gatctgcgat caggggggcg agtgcgatct gcaagaccag gcaatggcgt
     3361 atgggtttga tcgcagccgc ttcgaggaga ataagcgcgc cgtccgtgac aacgatttcg
     3421 gtccgctggt caagact
//
LOCUS       DATLJT010001574         1856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3369709,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001574 DATLJT010000000
VERSION     DATLJT010001574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369709"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VM910_36030"
     CDS             complement(<1..196)
                     /locus_tag="VM910_36030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67951.1"
                     /translation="MPTIRLKALGAIIAVVAAFVAALGQPAAAQDKVKVGVFPTASSL
                     PYFVAIERGFFKEQNIEPETI"
     gene            414..1100
                     /locus_tag="VM910_36035"
     CDS             414..1100
                     /locus_tag="VM910_36035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67952.1"
                     /translation="MHRLRQWRLVGSAVGGERHFWLERLIFGLDGMLRRWQSVIEYTH
                     DPTCILRIKFGRLDQDVVLSDGTAGHVGERFIDLHLWNEQIPAMPKEGASIAWAREMS
                     LCFQHSLRQLARYLAARSDLDDISLVRCTMEFAGRERNDQMVRLIRRYGFEFVASTTT
                     VTMGECARRLAENIFISLIVLARNQAALRRDTLRRGRTRVFMSRKVLEQRYGGGADSI
                     GPKPRPICPS"
     gene            1261..>1856
                     /locus_tag="VM910_36040"
     CDS             1261..>1856
                     /locus_tag="VM910_36040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI67953.1"
                     /translation="MLFRVGRTFAVAACVSVVAIEFAAAADMPYVTNAPPAALPPSWA
                     GFYLGGQVGYGADSVRWRNLGASAFFSPLNSVTRDHGSGVIGGGQLGYNFQYNRLVFG
                     IEGSASAADFDRSFASPYFPATDVWSSRLTWLTTVTGRVGYGFDNWLPYVKGGFAAGN
                     IETSIQTNPFGVISQGSAVHTGWTLGGGVEFKVAPQFSL"
BASE COUNT          318 a          571 c          598 g          369 t
ORIGIN      
        1 ggatggtctc aggttcgatg ttctgttcct tgaagaagcc gcgctcgatc gccacgaaat
       61 agggcagcga ggacgcggtc ggaaacacgc ccaccttcac cttgtcctgc gcggcggccg
      121 gctggcccaa ggcagcgacg aaggcggcga cgacggcgat gatcgccccc agcgccttca
      181 agcggatcgt cggcatcgca tcacctccgt cattccatcg cgccccaatg tctcgcccgc
      241 gcttgcctta gccgggggcg ggcggctcga tccatgagga cgagggcggc caacatccac
      301 caacttcagc gtcatggggc tattcgttgc tcgggggttg gtgcagattg ggagccggcg
      361 cagattaatc gattgtaata tcgcagcaag cgtgccgtta ggcatccgat gccatgcata
      421 ggttgcggca atggcggctg gtcgggtcgg ctgtgggggg agagcgtcat ttttggctgg
      481 agcggctcat cttcgggctg gacggcatgc tgcgccgctg gcagtccgtc atcgaataca
      541 ctcacgatcc gacttgcatt ttgcgcatca aattcggccg cctcgatcag gacgtcgtgc
      601 tttcggacgg cacggccggt cacgtcggcg agcgcttcat cgatctgcac ctgtggaacg
      661 agcaaatccc ggcgatgccg aaggagggcg catcgattgc atgggcgcgg gagatgagcc
      721 tttgcttcca gcactcgctt cggcaactcg cgcgctatct cgccgcgcgc tccgacctcg
      781 acgacatttc gcttgtgcgt tgcaccatgg agttcgctgg ccgcgagcgc aatgaccaga
      841 tggtgcgcct gatccgccgc tatggattcg agttcgttgc gtccacaacg acggtgacga
      901 tgggtgaatg cgcccgccgc ctggccgaaa acatcttcat ctccctgatc gtgttggcgc
      961 gcaatcaagc ggcgctgcgg cgagacacgc tacgccgggg ccggacacgg gttttcatgt
     1021 cacgcaaggt gctcgaacaa cgctatggcg gcggcgcaga tagcatcggt cccaagcctc
     1081 gcccgatctg cccatcctga ccgacgaagc gctgcctttg gatcggcgcg cggttcgagt
     1141 gaagagccac aacatttatt aaccttgtcg gaaaggaggc gcaccgaagc cgcgctttcg
     1201 ccaacgttgc cgacaagttt ccggaaaatc tggtgttggg ggcgaccagg gagaggccat
     1261 atgcttttcc gcgtcggtcg aacgtttgct gtcgctgctt gcgtgagcgt tgtggcgatt
     1321 gaattcgccg cagcagccga tatgccctac gtgacaaatg ccccgcccgc ggccctgcct
     1381 ccgagctggg ccggattcta tctgggtggc caggtcggct acggcgccga tagcgtgcga
     1441 tggcgaaatt tgggtgcgtc cgcgtttttt tcgccgctga attcggtgac gcgcgaccac
     1501 gggagcggag tgatcggcgg cgggcagctc ggctacaact ttcagtacaa ccggctggtg
     1561 ttcggaatcg agggatcggc atcggccgcc gatttcgacc gcagcttcgc cagtccgtat
     1621 ttccccgcga ccgacgtgtg gtcctcgcgg ctcacctggc tcaccacggt gaccggccgc
     1681 gtcggctacg gcttcgacaa ctggctgccc tatgtcaaag gcggcttcgc cgccggcaac
     1741 atcgagacca gcattcagac caacccgttt ggcgtgatct cgcagggatc ggcggtgcac
     1801 accggctgga cgctgggcgg cggggtcgag ttcaaagtcg cgccccaatt ttcgct
//
LOCUS       DATLJT010001575         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1303907,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001575 DATLJT010000000
VERSION     DATLJT010001575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303907"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VM910_36045"
     CDS             <1..1098
                     /locus_tag="VM910_36045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI67954.1"
                     /translation="GQKLNVALLIGGVSFGDQDSKLTRGVDVLIATPGRLLDHTERGG
                     LLLTGVELLVIDEADRMLDMGFIPDIERICKLVPFMRQTLFFTATMPPEIRRVTEAFL
                     HNPVKVEVSRPATAAVTVAQSQVPAGREPHEKRELLRRLLRGAKDLKNAIIFCNRKRD
                     VAVLHKSLQKHGFSVAALHGDMDQPARMAALDQFRRGELPLLVASDVAARGLDIPEVS
                     HVFNFDVPHHPDDYVHRIGRTGRAGRAGTAISIVTPLDQKSIAAIEKLIGQDIPRAEG
                     DFTTGADTSDDGDHRREQRSREGPRRGRKPQREARHGAGNRHHGTSTQSSASRHAPRN
                     ESRRAPRETEAEPADHSHLPAFLLRPVRDRV"
     gene            1283..2349
                     /locus_tag="VM910_36050"
                     /pseudo
     CDS             1283..2349
                     /locus_tag="VM910_36050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(2346..2771)
                     /locus_tag="VM910_36055"
     CDS             complement(2346..2771)
                     /locus_tag="VM910_36055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HVI67955.1"
                     /translation="MDRDFLIDLFSSFGPVTIRRMFSGYGISADGTNFALALRSGLYF
                     RADESTIPQFEAEGSKPFQYQARLKTVTVNSYWELPARLFDDSEELSVWARAALAAAQ
                     HAAMKKRTKARRPTKVAQQKPAATKAPAKVRRKKAANKR"
BASE COUNT          568 a          924 c          848 g          531 t
ORIGIN      
        1 ggccagaaac tcaacgtcgc gctcttgatc ggcggcgtct cgtttggcga tcaagactcg
       61 aaattgaccc gcggcgtcga cgttctgatt gcaactcccg gtcgcctgct cgaccacacc
      121 gaacgcggcg gccttttgct caccggagtc gagctgcttg tcatcgacga agccgatcgc
      181 atgctggaca tgggcttcat tcccgacatc gaacgcatct gcaagctggt gccgttcatg
      241 cgacaaaccc tgttcttcac cgcgacgatg ccgccggaaa ttcgacgcgt gacggaagcg
      301 ttcctccaca atccggtgaa ggtcgaggtg tcgaggccgg caaccgccgc cgtaaccgtg
      361 gcacagtcgc aggttcccgc gggccgcgag ccgcacgaga aacgcgagct gctgcgccgc
      421 ttgttgcgcg gcgcgaagga cttgaagaac gccatcatct tctgcaaccg caagcgcgat
      481 gtcgccgtat tgcacaagtc gctgcagaag cacgggttca gcgtcgctgc cctgcacggc
      541 gacatggacc agcccgcacg gatggccgcg ctcgatcaat tccgcagggg cgagttgccg
      601 ctattggtcg cttccgacgt cgcagcacgc ggccttgata ttccggaggt aagccacgtc
      661 ttcaattttg acgtccctca tcatcccgac gattacgttc atcgcatcgg ccgcaccggc
      721 cgcgcgggac gtgcgggaac ggcgatttcg atcgtcacgc cgctcgatca gaagtcgatc
      781 gccgcaatcg agaagctgat aggtcaggat attccccgcg ccgagggcga cttcacgacc
      841 ggcgccgata catccgacga tggcgaccac cggcgcgagc agcgctcgcg cgagggcccg
      901 cgccgcggac gcaaaccgca gcgcgaggca cgccacggtg ccggcaatcg tcaccatggc
      961 acgtcgacgc agtcgtctgc gtcacgacat gcgccgcgca atgaatcgcg tcgcgcgccc
     1021 cgcgagacgg aggcagagcc agccgatcat tcgcatttgc cggcattcct cttgcggccg
     1081 gttcgcgatc gcgtctagcg cggctttcgc tttgacgtgc ctgattgcag ctcgtcgttc
     1141 gcaatgcagg cgcttcaatc ggcaacatgc ttgcactgcg gaacccgttt accggtcatt
     1201 cattctcctc cactaacgtt ccccccgata attagagtta ttgctgcggc aggcggcccg
     1261 gcctgctatg gggacgtaat ccgtggtcaa ggtgctcgac gaacacgaac gcacgatggc
     1321 ctttgccgaa gtcgcgctcg ggcagatcag gtcgcttcgt caaaccgccg tgccccgcaa
     1381 ctacgaaatc tggtacatct acgcgacggg ttacaacgct ccgctcaaca agatcatcaa
     1441 cgagacgctg gcgcgcaacg gcaatttgac cgaagccgat ctcgagcaga tctacgagac
     1501 ctatctctcg cacatcaagg ccaccgatcg catcgacaag gtcggtgcgc gcgtgattgg
     1561 cgaaatcgac gatgtgatga gcctcctgac tgacgcactc ggcatgtcgg cgagttacga
     1621 tgccagtttg aacggcgcga gcgagaagct ttcggccgcc aagacccccg accaggtcaa
     1681 gggtgtcgtc gagtctctcc tgaggtcgac gcgcgagatg cgcgagacca acaaggctct
     1741 ggaagagcgg ttgatgctgt ccaagaacga aatcagcaat cttcagcaga gcctggaagc
     1801 gatccgcgcg gagagcctca ccgaccccct cacggggctc ggaaatcgca aagtttcgac
     1861 cgcatgatcg agacggctgt gcaggacgcg ctcgcgaccg gcgaaccgtt atcgcttctg
     1921 atgttcgaca tcgatcactt caagtcgttc aacgattcct acggccatct taccggcgac
     1981 caggtgctgc ggctggtcgg catgtccctg aagcagacga tcaagggtca ggacatcacg
     2041 gcgcgctatg gcggcgagga attcgcggta gtcttgccca gtaccgcgct gcgccaggca
     2101 cttaccgtcg ccgaccacat tcgccgcgcc gtgatggcga aggaattgaa gaagaaatcg
     2161 actggcgaaa tcctcggccg tgtcaccatt tcggtcggcg tgtccatgct gaagcccgga
     2221 gacgatcccg acgcgctgat cgagcgcgcg gacgcctgcc tctacgcggc caagcgcaac
     2281 ggccgtaacc gggtgatctg cgaggtcgac ccggaataca ccgccgaaat ccagagccag
     2341 gtggcttagc gcttatttgc agctttcttt cgtctcactt ttgcgggcgc tttcgtggcc
     2401 gccggctttt gctgggcaac ctttgtcggc cttcgcgcct tcgtccgctt cttcatagcc
     2461 gcatgctggg ccgcagccag tgccgccctc gcccagactg acagctcctc gctatcgtcg
     2521 aacagccgcg ccggcagctc ccagtaggag ttcaccgtga cggtcttcag gcgcgcttga
     2581 tattgaaacg gctttgatcc ttccgcctcg aactgcggaa tcgtcgactc gtcggcacga
     2641 aaatagagcc ccgatcgcag cgccagcgcg aaattcgtac cgtcagcgga aatgccgtag
     2701 ccggaaaaca tccggcggat ggtgaccggc ccgaaactcg agaacagatc gatcagaaaa
     2761 tcgcgatcca tggccgagcg cccgatgcca ctccctcata gtgaggagcg cttgcgcgcc
     2821 tcgaagcatg aggtcatatg cgcgggaacg gtgttagacc ttcgcaggct t
//
LOCUS       DATLJT010001576         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4289245,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001576 DATLJT010000000
VERSION     DATLJT010001576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4289245"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1409)
                     /locus_tag="VM910_36060"
     CDS             complement(30..1409)
                     /locus_tag="VM910_36060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HVI67956.1"
                     /translation="MNEHVHSDKTRVAAISVFASSGMAVAKLVVGLIIGSLALISEAL
                     HSSVDVVATVITWMVVRVSDLPADEEHHYGHGKFESISALGVIALLYVLAGGILVESY
                     SRLREGTNPPTISAVPFVVLLVDIAVNFWRARALHRAAQTTQSQALAADALHFASDVL
                     GSIAVIIGLALAGLGFWWGDAAAAIAVAIMIAVLGFRMARSTVETLLDRAPEGASEVA
                     RSAIRSVPGVVDVQRLRARKVGATYFIDSIVKVPRTYPIDRIDQIKQKAQAAVAAALG
                     DTDLTFTAAPVALDNESVRERIMVIARNSGLAVHHVTVHDLSGRLIVSIDLEVDGDMA
                     LTAAHDIAHNLERNIRDEFGADVEVDTHIEPLEPELPFGTDAPPDRIEIIRAALSRFA
                     ADTAIHDIHNVRVRDTEAGEIVNFHCRAAPSMSVIKVHETVDEIERALRRAFPTVKRV
                     ISHAEPLRA"
     gene            complement(1530..2498)
                     /locus_tag="VM910_36065"
     CDS             complement(1530..2498)
                     /locus_tag="VM910_36065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HVI67957.1"
                     /translation="MRAYVYGPNGPAITEVARPEAKGTQVLVKVKACGLNRADLGMTK
                     GHTHGAAGGVGTVLGMEWAGEVAALGPDARGVKVGDRIMGSGGAAFAEYTLADHGRLF
                     RSPSNMNFEEAATLPVALTTMHNAVVTNGALKAGQSVLIQGASSGVGLMAMQIAKLKG
                     ASLVIGSSTDAMRRSRLKEFGADLAVDSSDPGWVEQVLKATNGEGVDLIVDQVSGKVA
                     SQNLAATRIKGRIVNVGRLGGTRAEFNFDLHAARRITYVGVTFRTRTIEEIREIFDEV
                     KKDIWAAVESRRLQLPIDRVFSFDDIGRAFEHMEANKHLGKIVVTL"
     gene            complement(2525..3520)
                     /locus_tag="VM910_36070"
     CDS             complement(2525..3520)
                     /locus_tag="VM910_36070"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HVI67958.1"
                     /translation="MIRTRFTELAGVDHPIVQGGMQWVGRAELVAAVANAGALGFITA
                     LTQPTPEDLSKEIARCRDMTDKPFGVNLTILPAIKPPPYAEYRSAIIASGIRIVETAG
                     NKPQEHVTEFKKHGIKIVHKCTSVRHALSAERMGVDAISIDGFECAGHPGEDDTPGLI
                     LIPVAADKVKIPLIASGGFGDARGLVAALALGAEGINMGTRFMCTKESPIHQLIKERI
                     VANDERETELIFRTMRNTSRVAKNTISTKVVAMEKEGATFDQVRELVAGARGKMVYAT
                     GDADHGIWSAGQVQGLIHDIPSCAELVSRIMREAEAIIRSRLEGMMSAARREAAE"
     gene            complement(3613..>4054)
                     /locus_tag="VM910_36075"
     CDS             complement(3613..>4054)
                     /locus_tag="VM910_36075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HVI67959.1"
                     /translation="LDRIRDEIWLKLWGNVCFNPISALTHATLEEICTDPETRALSKA
                     IMLETQAIAESFGVKFRVDVERRIEGARKVGAHKTSMLQDLERGRPMEIDPLVTVVQE
                     MGRLTKIATPTLDLVLALVAQRGKTAGLYERIVRPAETKQPAFA"
BASE COUNT          680 a         1292 c         1312 g          770 t
ORIGIN      
        1 gaacgctgtg tgaccgccgc gttggcggct cacgcacgaa gcggctcggc gtggctgatg
       61 acgcgcttca cggtcgggaa ggcgcggcgc aatgcgcgct cgatctcgtc cacggtctcg
      121 tgcaccttga tcacgctcat cgaaggcgcg gcacggcagt ggaagttgac gatctcgccc
      181 gcctcggtgt cgcgcacccg cacattgtgg atgtcatgga tcgcggtatc ggcggcaaaa
      241 cgtgatagcg ccgccctgat gatctcgatg cggtcgggcg gagcgtcggt gccgaacggc
      301 aattccggct caagcggttc gatatgggtg tcgacctcga cgtcggcgcc gaattcgtcc
      361 cggatgttgc gttccaggtt gtgggcgatg tcgtgggccg cggtcagcgc catgtcgccg
      421 tcgacttcca gatcgatgct gacgatcagc ctgccggaca gatcgtgcac ggtgacgtgg
      481 tgtacggcga ggccggaatt gcgggcgatc accatgatgc gttcgcgcac gctctcattg
      541 tcgagcgcaa cgggcgcggc ggtaaatgtc agatcggtat cgccgagtgc ggcggcgacc
      601 gcggcttgcg ccttttgctt gatctgatcg atccggtcga tcgggtaggt gcgcggcacc
      661 ttcacgatcg aatcgatgaa ataggttgca ccgaccttgc gggccctcaa gcgttggacg
      721 tcgacgacgc cgggaaccga ccggatggcg cttcgtgcga cctcggaggc gccttcgggc
      781 gcgcgatcga gcagggtctc cacggtggag cgcgccatcc tgaaacccag cacggcgatc
      841 atgatcgcga ctgcgatcgc agcagcagca tcgccccacc aaaagcccag tccggcgagc
      901 gcaaggccga tgataacggc gatcgagccg agcacgtcgg aggcgaagtg cagcgcgtcg
      961 gcggcgagcg cctggctctg cgtcgtttgc gccgcgcggt gcagggcgcg cgcccgccag
     1021 aagttgacgg cgatatcgac cagcaagacg acaaatggca ccgccgagat cgtgggcggg
     1081 ttggttccct ccctcagtcg gctgtaggat tcgaccagga tacctccggc cagtacatag
     1141 agcagcgcga tcacgccgag tgcggagatg ctttcgaatt tgccgtggcc atagtgatgc
     1201 tcctcatccg cggggaggtc ggagacccga accaccatcc aggtgatgac ggtcgcaacc
     1261 acatcgaccg agctgtgcag tgcctcggag atcagcgcca gcgagccgat gatgaggccg
     1321 acgacgagct tggcgaccgc catgccggag ctggcaaaca ccgagatcgc cgcgacgcgg
     1381 gtcttgtcgc tgtggacgtg ctcattcatg agcgtttgct tagcagggcc aaggcccgca
     1441 cgaaagcgcg gaccggaacg cgctatcgcg gcccattcgc gggcatattg cgaattgttc
     1501 cgatttgaaa cgccttccgc gatccggcat caaagcgtca cgacgatctt gccgagatgc
     1561 ttgttggctt ccatgtgctc gaatgccctg ccgatatcgt cgaaggagaa caccctgtcg
     1621 atcggcagtt gcagcctgcg cgactccacc gcggcccaga tgtccttttt gacctcgtcg
     1681 aaaatctcgc ggatctcctc gatcgtgcgg gtgcggaagg tgacgccgac ataggtaatg
     1741 cggcgcgccg catggaggtc gaaattgaat tcggcgcggg tgccgccgag ccggccgaca
     1801 ttgacgatac gtcccttgat cctggtcgct gccaggttct ggcttgcaac cttgcccgag
     1861 acctgatcga cgatcagatc gacgccctcg ccattggtcg ccttcagcac ctgttcgacc
     1921 cagccgggat cggacgagtc gaccgcgagg tcggcgccga actccttcag gcggcttcgg
     1981 cgcatcgcgt ccgtcgagga gccgatcacc aacgaggcgc ccttgagctt ggcgatctgc
     2041 atggccatca gcccgacgcc ggagcttgcg ccctgaatca gaacgctctg gcctgccttg
     2101 agagcgccgt tggtgacgac cgcgttgtgc atggtcgtca gcgcgacggg gagggtggcg
     2161 gcctcctcga aattcatgtt cgagggcgag cggaacagcc gaccgtggtc ggcgagggtg
     2221 tattccgcga aggccgcccc gcccgaaccc atgatcctgt cgccgacctt gacgcccctg
     2281 gcatccggcc cgagcgcggc cacttcgccg gcccactcca tgccgagcac cgtcccgacg
     2341 cccccggccg cgccatgggt gtggcccttg gtcatgccga ggtcggcgcg attgagcccg
     2401 cacgccttca ccttcaccag cacctgcgtg cccttggctt ccggccttgc aacctccgta
     2461 atggcaggac cgttcggccc gtagacatag gctctcatgc gatcgttcct ttctcagcaa
     2521 aaggctattc cgcggcctct cgccgggccg ccgacatcat accttccagc cggctgcgaa
     2581 tgatggcttc ggcctcgcgc atgattcgcg aaacgagttc ggcgcagctc gggatgtcgt
     2641 ggatcaatcc ctgcacctga cctgccgacc agatgccgtg atcggcatcc ccggtcgcat
     2701 agaccatctt gccgcgcgcg cccgccacga gctcgcgcac ctggtcgaag gtggcgcctt
     2761 ccttttccat ggcgaccacc ttggtcgaga tcgtgttctt cgcaacgcgc gaggtgttgc
     2821 gcatggtgcg gaaaatcagt tcggtctcgc gctcgtcatt ggcgacgatg cgctccttga
     2881 tgagctgatg gatcgggctc tccttggtgc acatgaaacg cgtgcccatg ttgatgcctt
     2941 cggcgccgag cgcgagcgcg gccacgaggc ctcgcgcgtc gccgaatccg cccgaggcga
     3001 tcagcgggat cttcaccttg tcggcggcca cgggaatcag gatcaggccg ggcgtgtcgt
     3061 cttcgccggg atggccggcg cattcaaagc cgtcgatcga gatcgcatcg acccccatgc
     3121 gctcggccga cagcgcgtgg cggacgctcg tgcatttgtg cacgatcttg atgccgtgct
     3181 tcttgaattc ggtgacatgt tcctgcggct tgttgccggc ggtctcgacg atcctgatgc
     3241 cgctcgcgat gatggccgag cggtattcgg catagggcgg cggcttgatc gcgggcagga
     3301 tggtcaggtt gacgccgaac ggcttgtcgg tcatgtcgcg gcagcgggcg atctccttgg
     3361 aaagatcttc cggcgtcggc tgggtcagcg cggtaatgaa accgagcgca ccggcattgg
     3421 cgacggctgc aaccagctcc gcgcggccta cccattgcat gccgccttgc acgatcggat
     3481 ggtcgacacc ggcgagctcg gtaaatcgcg tcctgatcat tccgccctct gcttccctga
     3541 ggttttcagg ggcaggatgg cgtcacatca gccaaatgtc taccggccgc catggcggta
     3601 ggggcagggc catcatgcaa aagccggctg tttcgtttcc gccggacgga caattcgttc
     3661 atagagacca gccgttttgc cgcgctgggc gaccagcgcc agcaccaggt cgagcgtcgg
     3721 cgtggcaatt ttggtgaggc ggcccatttc ctgcacgact gtcaccaacg ggtcgatctc
     3781 catgggacgg ccgcgttcaa ggtcctgcag catcgaggtc ttgtgagcgc cgaccttgcg
     3841 cgcgccttcg atccgccgtt cgacgtcgac acggaatttg acgccgaagc tttcggcgat
     3901 cgcctgcgtc tccagcatga tcgcctttga cagcgcccga gtctcggggt ccgtgcagat
     3961 ctcctccagc gtcgcatggg tgagcgcgct gatcggattg aagcagacat tgccccagag
     4021 tttcagccag atctcatcgc gaatgcgatc gagc
//
LOCUS       DATLJT010001577         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3141837,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001577 DATLJT010000000
VERSION     DATLJT010001577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141837"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /gene="pyrH"
                     /locus_tag="VM910_36080"
     CDS             <1..231
                     /gene="pyrH"
                     /locus_tag="VM910_36080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455040.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVI67960.1"
                     /translation="NVDGIYSADPKKDRSAKRFDRLTHAQAIEGGYKVMDTTAFALAR
                     ETSLPIIVFSIAEPGSMGAILRGKGRGTIVAG"
     gene            284..847
                     /gene="frr"
                     /locus_tag="VM910_36085"
     CDS             284..847
                     /gene="frr"
                     /locus_tag="VM910_36085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVI67961.1"
                     /translation="MSTGKFDINELKRRMQGATQALKHELGGLRTGRASASMLEPVQV
                     DAYGAHMPLNQLATISVPEPRMISVQVWDKSMVKAVEKAIVDSNLGLSPATEGQVLRL
                     RIPELNQERRKELVKVAHKYAEAARVAVRHVRRDGLDAVKKLEKNHEISEDDQDRLAT
                     DVQKATDGMISEIDQMLAAKEKEILTV"
     gene            860..1618
                     /locus_tag="VM910_36090"
     CDS             860..1618
                     /locus_tag="VM910_36090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HVI67962.1"
                     /translation="MSNAAVPATQDHGRSDGPLHVAIIMDGNGRWAAARGLTRAQGHR
                     RGVEALRRVVRAAHELGIHYLTIFSFSSENWSRPASEIGDLFGLLRRFIRNDLATLHR
                     DGVRVRVIGEREGLEPDICALLNEAEELTRNNTSLNLVVAFNYGSRQEIAHAARRLAC
                     EVAEGRRDPASIDADTLGQYLDAPDIPDPDLIIRTSGEQRLSNFLLWQAAYSELVFVP
                     MHWPDFDKAALESAIAEYSRRERRFGGLAAKTAS"
     gene            1615..2460
                     /locus_tag="VM910_36095"
     CDS             1615..2460
                     /locus_tag="VM910_36095"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVI67963.1"
                     /translation="MSEGEAVPALAGPPSRNLLERILAAAVLVPLTLGAAYAGGVLWT
                     ALVTLAVIGLYVEWLTVAGAARERRIVATGVVALAVTGLSLSLSRLDAALVLGVLGLL
                     SVTFISAERRGWVVAGFVYAAVPLFAAVLLRADLQLGFIALIFVLLIVWTTDIGAYFA
                     GRGIGGPKLWPRVSPNKTWAGAIGGFAVSLLVAAGFAASGVSRILPLLIVGGALSIAG
                     QLGDLFESAVKRHFGVKDSGHLIPGHGGLMDRLDSLVAAIALAAILGILRGGVDGAGR
                     GLMVW"
     gene            2465..>2604
                     /locus_tag="VM910_36100"
     CDS             2465..>2604
                     /locus_tag="VM910_36100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVI67964.1"
                     /translation="MSAVPLRNNKAAVSAAERSVTVLGATGSIGDSTMDLLRGARDRY
                     RV"
BASE COUNT          450 a          784 c          845 g          525 t
ORIGIN      
        1 aatgtcgacg gcatctattc tgccgatccc aaaaaggacc ggtccgccaa gcggttcgac
       61 cgcttgaccc acgcgcaggc cattgaaggc ggctacaagg tgatggacac gaccgcattc
      121 gcgcttgccc gcgagacgtc gctgcctatc atcgtgttct cgatcgccga gcccggttcg
      181 atgggcgcga ttctgcgcgg caagggccgc ggaaccatcg ttgccggcta acagagccgc
      241 ggctttgcgc ctcgacggca agcttgcaga ggaggagagc gttatgtcca cgggtaagtt
      301 cgacattaac gaattgaagc gccgcatgca gggcgcgacc caggcgctca agcatgaact
      361 tggtggcctg cgcaccggac gcgcttctgc ttcgatgctg gaaccggttc aggtcgatgc
      421 ctatggcgcg cacatgccgc tgaaccagct cgcgaccatc agcgtgccgg aaccgcggat
      481 gatttcggta caggtctggg acaaatcgat ggtgaaggct gtcgaaaaag ccatcgtcga
      541 ttccaatctc ggcctgtcgc ccgcaacgga gggccaggtg ctccgcctgc gaatccccga
      601 gctcaaccag gagcgccgca aggagctcgt gaaggtcgca cataaatatg ccgaggctgc
      661 gcgtgttgcg gtccgtcacg tccgccgcga cgggctggat gccgtcaaga aactggaaaa
      721 gaatcacgag atttccgagg acgaccagga ccgtctggcc accgatgtgc agaaagcgac
      781 cgacggcatg atttcggaaa tcgatcagat gcttgcggcg aaagaaaaag aaatccttac
      841 ggtctgaagg gatcgaccga tgtcgaatgc cgccgtcccc gcaacgcagg atcacggtcg
      901 atccgatggc ccgctgcacg ttgccatcat catggacggc aacggccgct gggctgcggc
      961 gcgtgggttg acgcgcgcgc aaggtcatcg ccgtggtgtc gaagcactgc gccgcgtagt
     1021 ccgcgccgcg cacgaactgg gcattcacta tctgacgatc ttttcgttca gctcggaaaa
     1081 ctggtcgcga ccggcgagcg agatcggcga cctctttggc cttttgcgcc gcttcatccg
     1141 caatgacctg gcgacgctgc accgcgacgg cgtgcgcgtg cgcgtgatcg gcgagcgcga
     1201 aggtcttgag cccgacatct gtgcgctctt gaatgaagct gaggagctga cgcgaaacaa
     1261 taccagcctc aatctcgtag tcgccttcaa ctacggttcg cggcaggaaa tcgcgcatgc
     1321 ggcccggcgt cttgcctgcg aggtcgccga gggcaggcgt gatcccgcct cgatcgatgc
     1381 cgatacgctc ggacagtatc tcgacgctcc cgatattccc gatcccgatc tgatcatccg
     1441 gaccagcggc gagcagcggt tgtcaaattt cctgttgtgg caggcagcct acagcgaact
     1501 cgtgttcgtg ccgatgcact ggccggattt cgacaaggcg gctttggaaa gcgccattgc
     1561 cgagtattcg agacgcgagc gccgcttcgg cggccttgcc gcgaaaaccg catcgtgagc
     1621 gaaggtgagg ctgtgccggc gcttgcggga ccaccgtcgc gcaatctttt ggagcgcatc
     1681 ctcgccgctg cggtgctggt gccccttacg cttggcgccg cttatgcagg cggcgtcctg
     1741 tggaccgccc tcgtcacgct cgcagtcatc gggctctatg tcgaatggtt gacggtcgcg
     1801 ggcgccgcgc gcgagaggcg catcgtcgca acgggtgtgg tggctcttgc agtgaccgga
     1861 ttgagccttt cgctctcgcg attggacgcg gccctggtgc tgggcgtgct cggactgttg
     1921 agcgtcacct ttatctcggc ggagcgtcgc ggctgggtgg ttgcgggatt tgtctatgcg
     1981 gccgtcccgc tgtttgccgc ggtgctgctt cgtgctgatc tccagctcgg ctttatcgct
     2041 ctgatctttg tccttttgat cgtctggacc accgacatcg gggcctattt tgccggccgc
     2101 ggcatcggcg gtccaaaact ctggcctcgc gtcagtccga acaagacctg ggccggcgcg
     2161 atcggcggat ttgcggtgag ccttctggtt gcggccggat ttgccgcctc cggcgtgtcc
     2221 cggatattgc cgctcctgat cgtcggtggc gctttgtcga tcgcaggaca gctcggtgat
     2281 ctctttgaat ccgcagtcaa acgccatttc ggggtaaagg attccggcca tctcatcccc
     2341 ggacatggcg gactgatgga ccggctcgac agtctggttg cagcgatcgc gctggcggcg
     2401 attttaggaa ttttgcgcgg cggcgtggat ggcgccgggc gcggtcttat ggtttggtga
     2461 ggcaatgagc gctgttcctt tgcgtaacaa caaggctgcg gtatcggctg ccgagcgaag
     2521 cgtcaccgtg cttggcgcaa ccggttccat cggcgacagc acgatggatc tgctgcgtgg
     2581 cgcgcgcgac cgctacaggg tcga
//
LOCUS       DATLJT010001578         3628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1993108,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001578 DATLJT010000000
VERSION     DATLJT010001578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993108"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VM910_36105"
     CDS             complement(<1..384)
                     /locus_tag="VM910_36105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270522.1"
                     /note="allantoate amidohydrolase and N-carbamoyl-L-amino
                     acid amidohydrolase are very similar; the allantoate
                     amidohydrolase from Escherichia coli forms a dimer and
                     binds zinc ions for catalytic activity and catalyzes the
                     conversion of allantoate to (S)-ureidoglycolate and
                     ammonia; carbamoyl amidohydrolase from Bacillus sp.
                     converts N-carbamoyl amino acids to amino acids, ammonia,
                     and carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HVI67965.1"
                     /translation="MSRAASNLQIDSARLWGTIHETAQFGATAKGGVRRLTLSAEDKR
                     VRDWFRRACENASLEVHVDALGSMFGLRRGRDMSKPPVGLGSHLDTQPTGGKYDGVLG
                     TLAALEVVRTLNDAGIETEAPIVVCN"
     gene            674..1486
                     /locus_tag="VM910_36110"
     CDS             674..1486
                     /locus_tag="VM910_36110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HVI67966.1"
                     /translation="MVVFAAEQQIREPVLPSHKARLDCRFGMAAPTSGVANGYVQGNL
                     AILPEKQAADFHRFCQLNPKPCPIIGISDVGNPKIPSLGIDLDIRTDLPRYRVWRNGE
                     VLDEPTDIMAHWRDDLVAFVIGCSFSFEEALMAEGLPIRHIEREVRVPMYRTNIQCHS
                     AGPFAGPMVVSMRPFKPKDAIRAVQITTRFPSVHGAPVHLGHPHSIGIADIARPDYGD
                     PVPVADDEIPVFWACGVTPQAVIAAAKLPFAITHAPGLMLVTDLMNRDLAVL"
     gene            complement(1501..2181)
                     /locus_tag="VM910_36115"
     CDS             complement(1501..2181)
                     /locus_tag="VM910_36115"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HVI67967.1"
                     /translation="MTEAKAGETTIREDICRLGRSLFERGLTPGSSGNISVKLDDGGW
                     LVTPTNASLGFLDPARLSRLDASGRLLSGDAPTKEVPLHSALYQTRGGAARAVVHLHS
                     THSVALSMLPEIDPRAALPPMTAYYLMRCGATALVPYYRPGDPAVADAIKGLAGKYSS
                     VLLANHGPVVSGDTLEAAVFAIEELEETAKLYLLLRGLNPRYLSPEQVQDLAKVFGLS
                     LPEDHDHR"
     gene            2298..3218
                     /gene="ltnD"
                     /locus_tag="VM910_36120"
     CDS             2298..3218
                     /gene="ltnD"
                     /locus_tag="VM910_36120"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HVI67968.1"
                     /translation="MPTSAMPQIAVIGLGSMGFGMATSLKRKGFEVTGCDVSAEPVSR
                     FVKEGGKSARTPAEAAKAADIVISVVLNAAQTEAILFGKDGVAETLAKDAVFVSSATM
                     DPDFARKLAKQLEATGRHYLDAPISGGAQRAAQGELTILASGSAAAFARARPALDAMA
                     AKLYELGDSPGLGAAFKMVNQLLAGVHIVAAAEAITFAARQGLDIRKVYEVITASAGN
                     SWMFENRMLQVINGDYKPRSAVEIFVKDLGIVQDMARTARFPAPLASAALQMFLGASG
                     AGMGRDDDASVARLYARISGTALPGEPKQD"
     gene            3237..>3628
                     /locus_tag="VM910_36125"
     CDS             3237..>3628
                     /locus_tag="VM910_36125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVI67969.1"
                     /translation="MPRFAANLTMMFNEVPFLDRFEAAAKAGFTAVEFLFPYEHSPDD
                     VGKRLAANGLTQALFNLPPGDWAAGEKGFAALPDRFDDLKQSLHTALPYVKATGVKRL
                     HLMAGIASRDDRRAVEAFYKSVAWAAEF"
BASE COUNT          643 a         1160 c         1159 g          666 t
ORIGIN      
        1 gttgcagacg acgatcgggg cttccgtttc gatgccggca tcgttgagcg tgcgcaccac
       61 ttcgagcgcg gccagcgtgc cgagcacgcc gtcatacttg ccgccggtgg gctgcgtgtc
      121 gagatgcgag ccgaggccga ctggcggctt cgacatatct cgtcccctgc gcaggccgaa
      181 catcgagccg agcgcatcga catggacctc gaggcttgcg ttctcgcagg ccctgcggaa
      241 ccagtcgcgc acccgcttgt cttccgccga cagcgtgagc cgccgcacgc cgccctttgc
      301 cgtcgcgccg aattgggcgg tctcatgaat ggtgccccag agacgggcgg aatcgatctg
      361 cagattggac gcggcgcggc tcatgctagt attcccggct cgggggcggc aaatgggtag
      421 cgaacgtcat atccgctcca ctcgctgttc aatgccgcgc cggctccctt gcgtcaaccg
      481 cgccgccgga cttggcgatc tgtcggcaag gtggcaggtc gggtgataac cggaaatttc
      541 gcgacatgtg ggccgcgacg ggtccattgc cgaggcccgg cgaagggcta tatcttgatc
      601 aaacattgcg atcggacttt gatggcatag tcgggattga cggctttcgc agaggaatag
      661 gcaggtcact caaatggttg tctttgcagc agagcagcaa attcgcgaac ccgttcttcc
      721 cagccacaag gcgcggctcg actgtcgctt cggcatggct gcgcccacgt cgggagttgc
      781 gaacggctat gtccagggca accttgcgat cctccccgaa aagcaggccg ccgactttca
      841 ccgcttctgc cagctcaatc ccaagccgtg ccccatcatc ggcatctccg atgtcggcaa
      901 ccccaagata ccctcgctcg gcatcgacct cgacatccgc accgacctgc cgcgctatcg
      961 cgtctggcgc aacggcgagg tgctcgacga gccgaccgac atcatggcgc attggcgcga
     1021 cgatctcgtc gccttcgtga tcggctgctc attttcgttc gaggaggcgc tgatggcgga
     1081 ggggctgccg atccgtcaca tcgaacgcga ggtgcgcgtg ccgatgtatc gcaccaacat
     1141 ccagtgccat tcggcgggtc cgtttgccgg ccccatggtg gtgtcgatgc ggccgttcaa
     1201 gccgaaggat gcgatccgcg cggtacagat caccacgcgt tttccttccg tgcatggcgc
     1261 gccggttcac ctcggtcatc cgcattcgat cgggatcgcc gatatcgcca ggcctgatta
     1321 tggcgatccg gttccggttg cggacgacga gatcccggtg ttctgggcgt gcggcgtcac
     1381 gccgcaggcg gtgatcgcgg ccgcaaaact gccgtttgcg atcacgcacg cgccgggctt
     1441 gatgctggtg acggatctca tgaacagaga cctcgccgta ctttgatgca ggcgccccgc
     1501 ctagcggtga tcgtggtctt ccggtagcga aagcccgaac accttcgcca gatcctgaac
     1561 ctgctccggc gacagatagc gggggttcag accgcgcagc agcagataga gctttgcggt
     1621 ctcctccaac tcctcgatcg caaacaccgc ggcttccagc gtatcgccgg agaccaccgg
     1681 cccgtgattg gcgagcagca ctgacgaata tttccctgcg agtcccttga tcgcatcggc
     1741 gaccgcgggg tcgccggggc ggtaataggg cacgagcgcg gtcgcgccgc agcgcatcag
     1801 gtaataggcc gtcatcggcg gcagcgctgc gcgcgggtcg atctccggca gcatcgataa
     1861 cgcaaccgaa tgcgtcgaat gcagatgcac aacggcccgc gccgcgccgc cgcgggtctg
     1921 gtagagcgcg ctgtgcagcg gcacttcctt ggtcggggcg tcgccggaga gcagccgccc
     1981 ggacgcatcg agccgcgaca gccgcgccgg atcgaggaaa ccgagtgagg cgttggtcgg
     2041 cgtgaccaac cagccgccgt catcgagttt cacgctgata ttgcccgacg agcccggggt
     2101 cagcccgcgc tcgaacagcg agcggccgag gcggcagata tcctcacgga tcgttgtctc
     2161 gccggctttt gcctcggtca tggcgcttac tctattttct actttgtctc atgctttggc
     2221 agcgcggcca agccgtgata ggcaagagat caagagacaa agcaacaagc tccggatagc
     2281 agaccgaggg aagcgccatg ccgacatccg caatgccgca aatcgccgtg atcggcctgg
     2341 gctcgatggg ctttggcatg gcaacctcgc tgaagcgcaa aggtttcgag gtgaccggct
     2401 gcgacgtctc ggcggagccg gtcagccgct tcgtcaagga aggcggcaag agcgcccgga
     2461 cgccggcgga agctgccaaa gctgccgata tcgtgatcag tgtggttctc aacgctgcgc
     2521 agaccgaagc catcctgttc ggcaaggatg gcgtcgccga aacgctcgcc aaagatgccg
     2581 tgttcgtttc ctccgccacc atggacccgg atttcgcgcg caagctcgcc aaacagcttg
     2641 aagcaaccgg ccggcattat ctggatgcgc cgatctccgg tggcgcccag cgcgcggcgc
     2701 agggcgagct gacgatcctt gcctcgggca gcgcggcggc atttgccagg gcgcgccctg
     2761 cgctcgatgc catggcggcc aagctttacg aactcggcga cagtccgggt ctcggcgccg
     2821 cgttcaagat ggtcaaccag ctcctcgccg gtgtgcatat cgttgccgcg gcagaagcaa
     2881 tcacctttgc cgcgcggcag ggtcttgata tccgcaaggt ctatgaggtg atcaccgcgt
     2941 ccgccggcaa ttcctggatg ttcgaaaacc gcatgcttca ggtgatcaac ggcgactaca
     3001 agccgcgcag cgcggtcgag attttcgtaa aagacctcgg catcgtgcag gacatggcgc
     3061 gcacggcgcg ctttccggcg ccgctcgcct ccgccgcgtt gcagatgttt ctcggcgcgt
     3121 caggcgccgg gatgggccgc gacgacgatg cgtcggtggc gcggctctat gcccgcatca
     3181 gcggcaccgc cttgcccggc gagccgaagc aggactgaaa gacaaggatt tttccgatgc
     3241 cgcgttttgc cgccaatctc accatgatgt tcaacgaggt gccgttcctg gatcgcttcg
     3301 aggcggcggc aaaagcgggc tttaccgcag tggagttctt gtttccttac gaacattcac
     3361 ccgatgatgt cggcaagcgg ctcgccgcga acggcctgac gcaggcgctg ttcaacctgc
     3421 cgccgggcga ttgggccgcc ggcgaaaaag ggtttgccgc gctgccggat cgcttcgacg
     3481 atctcaagca aagccttcat acggcgctgc cttatgtcaa ggcgaccggc gtcaagcgcc
     3541 tgcacctgat ggcggggata gcgagccggg acgatcgaag agcggtggag gcattttaca
     3601 aatccgtcgc gtgggccgcc gaattctt
//
LOCUS       DATLJT010001579        11417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_840962,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001579 DATLJT010000000
VERSION     DATLJT010001579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840962"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VM910_36130"
     CDS             <1..358
                     /locus_tag="VM910_36130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI67970.1"
                     /translation="FIAEIVRSGIQAVPRGQWDAAVALGLRRSFVLRQIVLPQALRLI
                     VPPLTSQYLNLTKNSSLAVAIGYQDIVSIANTTLNLTGQAIEAIALIMLVFLTISLSI
                     SLFMNWYNARIALSER"
     gene            409..1485
                     /locus_tag="VM910_36135"
     CDS             409..1485
                     /locus_tag="VM910_36135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI67971.1"
                     /translation="MQSGSSVAGRALRWLRANLFSSISSSLVTLVLLVLLVEALLSFV
                     RWGILDAVWTVPGSDSSACRAARGLGACWAVIGEKFRFILFGTYPFDQQWRPALAILV
                     FIALFYLSTRRSLWRRELIYVWAAALVAIGVLMWGGVLGLPFVSQERWGGLPVTLILA
                     TFGLAFGFPLGILVALGRRSKLPAIRSLCVLYVELIRGVPLISLLFMASVMFPLFMPD
                     GFNIDKLLRAQIAIVLFAGAYLAEVVRGGLQAVPRGQHEAAEALGLSYWQKNALIVLP
                     QAIRHVIPPLVNTFIAFFKDTSLVLIIGIFDLLTTAKTAVIDPAWQQFSVEVYIFVAI
                     IYFAFCFAMSRYSRHLETQSGPAK"
     gene            complement(1518..2912)
                     /locus_tag="VM910_36140"
     CDS             complement(1518..2912)
                     /locus_tag="VM910_36140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HVI67972.1"
                     /translation="MPDLWAVFGIAGLSIAYSANRAAPYNSGMVVRRPPHQIIAKQAD
                     RPDRASVVLCRRLSFGALLAVLLMLPSPYAQSQTIDKPHDTTAPTDAAAAGAFKSCIA
                     EIAPDALRRVSQKTFDAATSGLEADPQILDQSQSQPEFNQPIWQYLDAIVNEDRIAAG
                     RVVLLGLRPLFNAVEEAFGADRFIIAAIWGVESNFGTDAGSYPVIRATATLACMGRRQ
                     AYFRNEFIAALEILERGDVAPDRFKGSWSGAFGGTQFMPTTFLRFAVDFDGNGRRDII
                     DDVPDILASTANVLRAYGWTRDKPCAVEVTLPPGFDYSLTSERKPIAEWTHLGVVPSS
                     EARPLDQRDEANLWLPAGHEGPAFLLSNNFEAIKKYNTAEAYALAVCQLADRLRGGGP
                     LSKPWPRDERVLSAAERLELQSLLKSQGFDVGPIDGMMGIQTRAAIRQFQTKSNLVPD
                     GFPSAMLLERLRHP"
     gene            3030..3770
                     /locus_tag="VM910_36145"
     CDS             3030..3770
                     /locus_tag="VM910_36145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI67973.1"
                     /translation="MARSSKPTIDRTSQIYRALRHAIMEQALSPGAKLPEDAIGERFG
                     ASRTIVRHALGQLAAEGLVELRRNRGAVVATPAWEEARDIFDIRLGLERLVMSRLAGR
                     LDQAQIAQLKAHVADEERARGENEPLSIRLATQFHIVLANMTGSAAIARYVSEVSSRF
                     GLILALYSRPHSSECAVSEHRAVIDALVKGDAEVAMKVMENHLEAVANRALIMPTIRK
                     NRELSDILDSYAGEAQPARMPPPKPRKR"
     gene            complement(3794..4216)
                     /locus_tag="VM910_36150"
     CDS             complement(3794..4216)
                     /locus_tag="VM910_36150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVI67974.1"
                     /translation="MPAAAPALSAQTSDDVAIVRDFLDASMKPDAERAASHIAADFKL
                     TFTGGRTFDHPSGSIGFNAKRYRWVKKAMDRFDVVPGTDETIVYSIGTLYGEWPDGTA
                     FKGNRYIDRFVVRGGKITRMDVWNDSAERILERNGIDA"
     gene            4444..5619
                     /locus_tag="VM910_36155"
     CDS             4444..5619
                     /locus_tag="VM910_36155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67975.1"
                     /translation="MSAFLRSLSRRALLVGAAIVFSVPALAEDTIKFGLVAAMSGQSA
                     KSGEAIMRGLSLAMDEINANGGVLGKKLELVVRDDESNPAKGVVAARELVQREKVAVL
                     FGGLDTPVSMAIVPFANQSKVPFMGVWAAGTAITRNGAADNYVFRVSAVDALVDRALV
                     DYAMKKYDIKKPGMILINNPWGESNEGGLKAALADKGLPFAGIEKFQDADVDVVPQLT
                     RLKDAGADALFLVANVAPSSQVVKSLDRMGWNVPVVSHWGPAGGRFTELAGPSAAKVH
                     FIQTFSFAGKTSPKAEAVFAALKKKYPEIKTLADVTPAVGIANAYDAMHLTAIAVAKA
                     GSTDGTKIRDGFLAIDHYDGLIKAYDKPFSEQNHDALSSGDYIFTHFVEGEIVPLTN"
     gene            5683..6552
                     /locus_tag="VM910_36160"
     CDS             5683..6552
                     /locus_tag="VM910_36160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI67976.1"
                     /translation="MLFSAIVSGVAVGSMYGLIALGYHVTYVVSNTVNFAQGSSVMLG
                     AVLGYVLAIRLGLPMPLAAAIALLLCGLFGLLVERALVRPFAERGSNAWLMATVAGGI
                     VVDNVVLFTFGKEPRGFPSFLAQKPIEIFGTGIFPLQLVIPLVGLGLAVVLQFAFQRT
                     SKGKALLAVVQNKRAAQLMGINVRSAIALSFALSTALAGLAGILIAPLFSVQSDMGTL
                     FGIKAFAAAILGGISSAWGVMLAGILYGLIEAMVTSFAGSSYTQIAAFAVVIAALAIR
                     PNGLLGRAGTRKV"
     gene            6590..9061
                     /locus_tag="VM910_36165"
     CDS             6590..9061
                     /locus_tag="VM910_36165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HVI67977.1"
                     /translation="MAIIAFAAGGIAVALTSESYTHFVLALVALTTIVGVGLNVLLGL
                     SGQVSLGHVGFYAIGAYVSAILLGKSVSFWLALPLAGALAGIVGILLALPALRVAGPY
                     LAMITIAFAFIVQHGTIEWKSLTGGQNGLMSFAAPTFFGRPFAERETAILAVLLAAAS
                     LYLFRRFSKSAWGRSMLAVRDSETAARSVGLNPVTIKTASFAISAVLAGLAGAVYSPL
                     MMFVAPDSFPFSQSILFLLAVIVGGAGSLFGPAVGAAITVLLPELLSQLAEYRLLFVG
                     ALLLAVLWAAPDGVIGLFTRFLPKSSGKDNAAHPDIDLLSFLSGTGSAEELVVKGIGI
                     SFGGVKAATDVSFVASPGRITSLIGPNGAGKSTVLNMIGGFYRPDTGSVTRAGVELAG
                     EPAWRVARNGIARTYQTTQLFGAMTVLDNILIALRRGRLGNPLAALARPDDEAIARSL
                     LAFTGYRGRTDLEARYLAHVDLRLVEIARALATRPRVLLLDEPAAGLLRSDKDQLGAL
                     LKKIAASGVAVILVEHDVQLVMAISDHIVVLDAGAVIASGEPGSVRNDPAVLKAYLGA
                     SDMQARPRPQAWQAPPDEVILSCLKLDAGYGPAPVLQNVSLAVRSGEMVALLGANGAG
                     KSTAMLSMSGLLRPVDGAIILDDERIDGLPAHLIAARGLALVPEGRQMFTELTVRDNI
                     LLGAYTRPDADARTEVEALLRRFPRLRDRVDSRAGLLSGGEQQMVAIARGLMAKPRIL
                     LLDEPSLGLAPAMISELFAVLADLRDEGVTILLVDQMAALALKVADRGYVLESGKIVH
                     EDSSEALAHDPALEAAYLGKMEAVH"
     gene            9064..10302
                     /locus_tag="VM910_36170"
     CDS             9064..10302
                     /locus_tag="VM910_36170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI67978.1"
                     /translation="MGDDFILRNARVAGRGGEIFDILSRDGAIAEIAPRIATDARSED
                     VDGRLVLGGFVDSHIHLDKSCILDRCHIRHGTLEEAIGQVAAAKRAFTEEDVYVRASR
                     TLEKAILQGTTAMRTHVEVDARIGLTSFNAIRKLRDDYAWAIDLELCVFPQEGMINDP
                     GTEEMLVAACEAGADLIGGCPYADSDRARHIEHIFGIARRFDLDIDFHLDFDLDPSWT
                     DMEEVCLQAEAHRYGGRVAIGHVTKLSSMPSAEFETWARRLADAGVAVTVLPSTDLFL
                     TGRDHDHNIPRGVAHAHRLSAQGVTCSLATNNVLNPFTPFGDCSLIRIANLYANVAQL
                     GDGPALCSCFDMVTRGPASLMNLSQYGVEVGNPADLVVLDCSEEAAAVAEIVQPLVAY
                     KRGRRSFSRAQPVLHRPNYG"
     gene            10424..10864
                     /locus_tag="VM910_36175"
     CDS             10424..10864
                     /locus_tag="VM910_36175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67979.1"
                     /translation="MTYKMNTALIASLSVVALMLAADDQTFARSGAGRGGVVSARAIS
                     HPFRHHPRNFGNFGGFGAFWPGDFANGSYGPNYGEPMLSPPQATSSDIRYTQTYDVPW
                     DWAHRFPPNVTPSDRPYVSSCPVETLTFHQHDGADKTVNVMRCY"
     gene            11014..>11417
                     /locus_tag="VM910_36180"
     CDS             11014..>11417
                     /locus_tag="VM910_36180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HVI67980.1"
                     /translation="MTDGPIDGGKAARPKLFQPFAIRGLTLKNRLVVPPMVHYRAEPG
                     NTCGTFHLVHLGRYALGGFGLVFVEATAVEEVGLINEHDLGIWNEAQAESFKPLIAFM
                     RRQNTAIGIQLAHGGRKSSSQTAMQGMGPLTE"
BASE COUNT         1955 a         3599 c         3633 g         2230 t
ORIGIN      
        1 cttcatcgcc gaaatcgtcc gcagcggcat tcaagccgtt ccgaggggcc aatgggacgc
       61 cgcggtcgcg ctgggcctgc gccgcagctt cgtgctccgg cagatcgtct tgccgcaggc
      121 gttgcggctc atcgtgccgc cgctgacgag ccaatatctc aatctgacca agaactcgtc
      181 gctcgcggtc gcgatcggct accaggacat cgtgtcgatc gcaaacacga cgctgaacct
      241 gacggggcag gccatcgaag cgattgcgct gatcatgctg gttttcctga ccatcagcct
      301 gagcatcagc ctgttcatga actggtacaa tgcgcgcatc gcgctgtcgg agcgctgatc
      361 gtgatctcgg ccgccgatac cccgcgaagc ccgcgctcat tcagcgatct gcaatcgggc
      421 tcaagcgtgg cgggccgcgc gctgcgctgg ctgcgcgcca acctgttttc gtcgatctcc
      481 tcgagccttg tcacgctcgt gctgctggtg ctgctcgtcg aggcgctgct cagctttgtg
      541 cgatggggca ttctcgatgc ggtctggacg gtgcccggca gcgactccag cgcctgccgt
      601 gccgcgcgcg ggcttggcgc atgctgggcg gtgatcgggg aaaaattccg cttcatcctg
      661 ttcggcacct atccgttcga tcagcaatgg cggccggcgc tcgcgattct ggtcttcatc
      721 gcgttgttct acctgtcgac ccggcgcagt ctttggcggc gcgagctgat ttatgtctgg
      781 gctgccgcgc tcgtcgcaat cggcgtcctg atgtggggcg gcgtgctcgg cctgcctttc
      841 gtgtcgcagg agcgctgggg cggcctgccg gtgacgctca ttctggcaac gttcgggctt
      901 gccttcggtt tcccgctcgg catcctcgtt gcactcggcc ggcgttcaaa acttcccgcg
      961 atccgctcgc tctgcgtgct ctatgtggag ttgatccgcg gcgtgccgtt gatcagtctc
     1021 ctgttcatgg cgagcgtgat gtttccgctg ttcatgcccg acggcttcaa catcgacaag
     1081 ctcctgagag cccagatcgc aatcgtgctt tttgccggcg cctatctcgc ggaggtggtg
     1141 cgcggcggct tgcaggcggt gccgaggggg cagcacgagg cagccgaggc gcttggcctg
     1201 tcgtattggc agaagaacgc cctgatcgtc ctgccgcagg cgatccgtca cgtcatccca
     1261 ccgctggtga acacctttat cgccttcttc aaggatacga gcctggtgct gatcatcggc
     1321 atcttcgatc tcttgaccac ggccaagacc gcggtgatcg accccgcctg gcagcaattc
     1381 agcgtcgagg tctacatctt cgtggcgatc atctatttcg cgttctgttt tgccatgtcc
     1441 cgctacagcc ggcatcttga gacgcagagc gggccggcaa agtgatgcct tctccatcgc
     1501 aagcccgggc ggccatctca tggatggcgg agacgctcga gcagcatcgc cgagggaaat
     1561 ccgtcgggaa caagattgga tttcgtctga aactggcgaa tcgcggccct ggtctggatg
     1621 cccatcattc catcgatcgg tccgacatcg aacccttgcg acttcagaag gctctgaagt
     1681 tcgagccgct cagcggccga gagcacccgc tcgtcgcgtg gccagggctt tgaaagcggt
     1741 ccgccgccac gaagccggtc ggcaagttga cacacggcaa gggcgtaggc ttcagcggta
     1801 ttgtacttct tgatggcttc gaaattgttc gacagcagga acgcgggccc ttcatgcccc
     1861 gcaggcaacc aaaggttcgc ttcgtcgcgc tgatcgagcg gtctcgcctc gctcgacggg
     1921 accactccaa ggtgcgtcca ttcggcgatc ggcttgcgct cggaggtcag ggaataatcg
     1981 aaccccgggg gcagggtgac ttcgacagcg caaggtttgt ccctcgtcca gccgtacgcg
     2041 cggagcacgt ttgccgtcga ggccaggata tcgggcacgt catcgatgat gtcgcgccgt
     2101 ccgtttccgt caaaatccac cgcgaaccgc aggaacgttg tcggcatgaa ttgcgtgccg
     2161 ccaaaagcgc cggaccacga gcccttgaag cgatcgggtg cgacgtcgcc tcgctccagg
     2221 atttcgagcg cggcaataaa ttcgtttcga aaataggctt gccgccgtcc catgcaggcg
     2281 agcgtcgcgg tggcacggat gacgggatac gaaccggcgt ccgtcccgaa gtttgattcc
     2341 acaccccaga tcgcggcgat gatgaagcga tcggctccaa aagcttcttc gacggcattg
     2401 aagagaggtc gaagacccaa cagcaccact cggcccgcgg caattctgtc ttcgttcacg
     2461 atcgcgtcca gatactgcca gatgggctgg ttgaactcgg gctgcgattg cgactgatcg
     2521 agaatctgag gatccgcttc cagcccggac gttgccgcat cgaaggtctt ctgcgaaacg
     2581 cgacgcagcg cgtcgggtgc gatctcggcg atgcaggact tgaaagcgcc ggccgccgct
     2641 gcgtccgtcg gcgctgttgt gtcgtgaggc ttgtcgattg tctggctctg cgcgtaagga
     2701 gagggaagca tgagaagcac cgcaagcagt gcgccgaagc tcaacctccg gcacaacacg
     2761 acggacgccc gatccggcct gtcagcctgt ttcgcgatga tctgatgcgg cggccgccgg
     2821 acaaccattc cggaattata gggtgccgcg cgattggcgc tatatgcaat cgacaagccg
     2881 gcaatgccga acaccgccca aaggtcgggc atcggttggc tccaggccgc gaagcgaccg
     2941 cacaaagtcg gggtcgccgt ccgaacgcta gtcttgacgc gcggcgcact tttgctcttg
     3001 tctgcccggc aaccagacca agcaacctca tggcgagaag ctcaaagccg accatcgatc
     3061 gcaccagcca gatctatcgg gcgcttcgcc acgcgatcat ggagcaggcg ctgtcgccgg
     3121 gtgcgaaact gcctgaggac gcgatcggcg agcgtttcgg tgcaagccgt accatcgtgc
     3181 ggcacgcgct cggacaactc gcggccgaag ggctggtcga gctgcggcgc aatcgcggcg
     3241 cggtggtcgc gacgccggcg tgggaagagg cgcgcgatat cttcgacatt cgcctcggcc
     3301 tggaacggct ggtgatgtcg cggctcgccg gccggctgga tcaggcgcag attgcgcagc
     3361 tcaaggcgca tgtcgccgac gaagagcgtg cgcgcggcga aaacgaaccg ctgtcgatca
     3421 ggctcgcgac gcaattccac atcgtgctcg cgaacatgac gggaagtgcc gcgatcgcca
     3481 gatatgtgag cgaggtttca tcgcgtttcg ggttgatcct tgcgctctac agccggcccc
     3541 attcctccga atgcgcggtc agcgagcatc gcgccgtgat cgacgcgctg gtcaagggcg
     3601 acgccgaggt cgcaatgaag gtgatggaaa accatctcga ggccgtggcc aaccgtgcct
     3661 tgatcatgcc cacgatccgg aagaaccgcg agctcagcga cattctggac agctatgccg
     3721 gcgaagccca gccggcgcga atgccgcctc cgaaaccgcg caagcgatag cagcccgatt
     3781 gcgcatgggc agtttacgcg tcgatgccgt tgcgctccag gatacgctcg gcgctgtcgt
     3841 tccagacgtc catgcgcgtg atcttgccgc cgcgaacgac gaagcggtcg atgtaacggt
     3901 tgcccttgaa ggcggtgccg tcgggccatt cgccgtagag ggtgccgatc gagtagacga
     3961 tggtctcatc cgtgcccggc acgacgtcga agcgatccat ggcctttttc acccagcggt
     4021 aacgtttggc attgaaaccg atgctgccgc tcggatggtc gaaggtacgt ccgccggtga
     4081 aggtcagctt gaagtccgcg gcaatgtggc ttgccgcacg ttcggcgtcc ggcttcatcg
     4141 atgcgtcgag aaagtcgcgc acgatcgcga cgtcgtcgga cgtttgagcg gaaagagcgg
     4201 gtgcggcggc cggcatagct ggtcctcctt cggggtttct gacctaagga tgcggttgtg
     4261 cgcgccgcaa ggaaagctaa aatcgcatgc acgattgcat acaatttgga cagtttttgc
     4321 acgcgaaaaa tcggctaacg gaaagcgcaa tctggtcaag cggctgagat cactgctatt
     4381 tccgatagtt cggccttggc acggcacttg ccacaagaat cccgtctaac ggaggaccac
     4441 ccaatgtcag ctttcctgcg atccctgtcg cgacgcgcgc ttctggttgg cgctgcgatc
     4501 gtgttttcgg tgccggcgct cgccgaggac accatcaaat tcggcctggt tgccgcgatg
     4561 tccggccaat cggccaagtc gggcgaggcc atcatgcggg gcctgtcgct tgcgatggac
     4621 gagatcaacg caaacggggg cgtgcttggc aagaagctcg agctggtcgt ccgcgacgac
     4681 gagagcaatc ccgccaaggg cgtggttgcc gcgcgcgaac tggtccagcg cgagaaggtc
     4741 gcggtgctgt tcggcggcct cgacaccccg gtgtcgatgg cgatcgtgcc gttcgccaat
     4801 cagagcaagg tgccgttcat gggggtgtgg gcggcaggca ccgcgatcac ccgcaacggc
     4861 gcggccgaca actatgtctt ccgcgtctcc gccgtcgacg ccctggtcga tcgggcgctg
     4921 gtcgactacg ccatgaagaa atacgacatc aagaagccgg gcatgatcct gatcaacaat
     4981 ccctggggcg aatccaacga gggcggcctc aaggcggcgc tggccgacaa gggtctgccg
     5041 tttgccggca tcgagaaatt ccaggacgcc gacgtcgacg tcgttccgca gttgacgcgc
     5101 ctgaaggatg ccggcgccga cgctttgttc ctcgtagcga atgtcgcgcc atcttcgcag
     5161 gtcgtgaaat cgctggaccg catgggctgg aacgtcccgg tcgtctccca ctggggtccg
     5221 gcaggcggac gcttcaccga actcgccggt ccgagcgcgg cgaaggtaca ttttatccag
     5281 accttcagct tcgcgggcaa gacgtcgccg aaggccgagg ccgtattcgc ggcgctgaag
     5341 aagaaatatc cggagatcaa gacgctcgcc gatgtcacgc ccgcggtcgg tatcgccaac
     5401 gcctacgacg ccatgcacct gacggcaatc gcggtggcga aggccggctc gaccgatggc
     5461 accaagatcc gcgacggttt ccttgccatc gatcactatg acggcctgat caaggcctat
     5521 gacaagccgt tctccgagca gaaccacgat gcgctgagtt ccggcgacta catcttcacg
     5581 catttcgtcg aaggtgaaat cgtgccgctc acgaattagg ctcgcatccg cgggacgttg
     5641 gatcgacctg cgtcccgcgg gcagcaagaa gcggtttcga tgttgttgtt ttcagcgatc
     5701 gtcagcggcg tcgccgtcgg cagcatgtac ggcctgattg cgctcggcta ccacgttacc
     5761 tacgtcgtct cgaacacggt caacttcgcg cagggctcct cggtgatgct cggggccgtg
     5821 cttggctacg tgcttgcgat ccgcctcggc ctgccgatgc cgctggcggc cgcaatcgcg
     5881 cttctgctct gcggcctgtt cggccttctc gtcgaacgcg cgctggtgcg gccgttcgcc
     5941 gaacgcggat cgaacgcgtg gctgatggcg acggttgcgg gcggcatcgt cgtcgacaac
     6001 gtcgtgctct tcaccttcgg caaggagccg cgcggctttc cgtcgttcct ggcgcagaag
     6061 ccgatcgaga tcttcgggac gggcatcttt ccgctgcagc ttgtgatccc gctggtcgga
     6121 ctagggcttg cggtagtgct gcagttcgcg ttccagagga caagcaaagg caaggcgctg
     6181 ctggcggtgg tgcagaacaa gcgcgcggcg caactgatgg gcatcaacgt gcgcagcgcc
     6241 atcgccctgt cctttgcgct ttcgacggcg ctggcgggac tagccggcat cctgatcgcg
     6301 ccattgttca gcgtgcagtc ggacatgggc acgttgttcg gcatcaaggc ctttgcggcg
     6361 gcgatccttg gcggaatcag ttcggcctgg ggcgtgatgc tggccggaat cctctacggc
     6421 ctgatcgagg ccatggtcac ctcgttcgcc ggctcgagct acacccagat cgcggccttt
     6481 gccgtcgtca tcgcagcgct tgccattcgc ccgaacgggc tgcttggccg tgccggaacg
     6541 agaaaggtat aacgtgacgc gcgcccggcc cctgccatcg atcgagacgc tggcgattat
     6601 cgcgttcgcg gccggcggca tcgcggttgc gctgacttcg gaaagctata cgcatttcgt
     6661 ccttgcgctc gttgcgctga cgacgatcgt gggtgtcggg ctcaacgtgc tgcttggcct
     6721 gagcggacag gtctcgctcg gccatgtcgg cttctacgcg atcggcgcct acgtctcggc
     6781 catcctgctc ggcaaaagcg tgagcttctg gctcgcgttg ccgcttgcgg gtgcgctcgc
     6841 cggcatcgtc ggcatcctgc tcgcactgcc ggccttgcgc gtcgcgggac cctatcttgc
     6901 gatgatcacc atcgccttcg ccttcattgt ccagcacggc acgatcgagt ggaagtcgct
     6961 caccggcgga cagaacggtc tgatgagctt tgcggcgccg acatttttcg gtcggccttt
     7021 cgcagagcgg gaaaccgcta tcctcgcggt gctgcttgcc gccgcatcgc tctatctttt
     7081 tcggcgattt tcgaaaagcg cgtggggccg ttcaatgctt gctgtacgcg acagcgaaac
     7141 ggcggcgcga tcggtcgggc tcaatccggt gacgatcaag accgcgtcat ttgcgatctc
     7201 ggcggtcctg gcaggacttg ccggcgccgt gtattcgccc ctgatgatgt tcgtcgcacc
     7261 cgactccttt ccgttttcgc aatcgattct gttcctgctc gcggtcatcg tcggcggcgc
     7321 gggctcgctg ttcgggccgg cggtcggtgc ggcgatcacg gttctgctgc ccgaactgct
     7381 gtcgcaactg gcggaatacc ggcttctctt cgtcggcgcg ctcctgctcg ccgtgctctg
     7441 ggcagcgccc gatggcgtca tcggtctgtt cacgcgattc ctgccgaaaa gcagcggcaa
     7501 ggacaacgcg gcacatccgg atatcgatct actctcattt ctctccggga ccggatccgc
     7561 ggaggaattg gtggtgaagg ggatcggcat ctcctttggc ggggtcaagg ccgcaaccga
     7621 tgtcagtttt gtcgcgagtc ccggccgcat caccagcctg atcggcccca acggcgccgg
     7681 caagtccact gtgctcaaca tgatcggcgg cttctatcgg cccgacaccg gcagcgtgac
     7741 gcgagccggc gtcgaactcg cgggcgagcc ggcctggcgc gtcgcgcgca acggtatcgc
     7801 gcggacctac cagaccacgc agttgttcgg cgccatgacc gtgctcgaca acatcctgat
     7861 cgctctgcgc cgcggccggc tgggcaatcc gctggccgcg ttggcacggc cggacgacga
     7921 ggcgatcgcg cgatctctgc tcgctttcac cggctatcgc ggccgcacgg atcttgaggc
     7981 gcgttacctc gcgcatgtcg atctcaggct tgtcgagatc gcccgcgccc tcgcgacgcg
     8041 cccgcgcgtg cttctgctcg acgagccggc ggccggcctc ctgcgatcgg acaaggacca
     8101 gctcggcgcg ctcctgaaga agatcgccgc aagcggcgtc gcggtcatcc tggtcgagca
     8161 cgacgtgcaa ctcgtgatgg cgatctcgga ccacatcgtg gtgctcgatg ccggcgccgt
     8221 cattgcgtcc ggcgaacccg gcagcgtccg caacgatccc gcggtgctca aggcctatct
     8281 cggtgcgagc gacatgcagg cgcggccccg gcctcaagcc tggcaggcgc cgccggacga
     8341 ggtgatcctg tcctgcctca agctcgacgc gggatatggc cccgcgccgg tcctgcaaaa
     8401 cgtctcgctt gcggtgcgct ccggcgagat ggtggcgctg ctcggcgcca atggcgccgg
     8461 caaatcgacg gcgatgcttt cgatgagcgg gcttttgcgt cccgtcgacg gcgcgatcat
     8521 tctcgatgac gagcgtatcg acggcctccc ggctcatctg atcgcggcgc ggggattggc
     8581 gctggtgccc gaggggcgcc agatgttcac cgaactcacc gtgcgcgaca atatcctgct
     8641 cggcgcctat acgcggccgg atgccgacgc gagaacggaa gtcgaagctc tcttgcggcg
     8701 atttccgcgg ctgcgcgatc gcgtcgacag ccgtgccggg ctgctttccg gcggcgagca
     8761 gcagatggtc gcgattgcgc gcggactgat ggcgaagccc agaatcctct tgctcgacga
     8821 accctcgctt ggcctcgcgc cggcaatgat cagtgagctg ttcgcggtgc tggccgacct
     8881 tcgcgacgag ggcgtcacca ttcttttggt cgatcagatg gccgcgctcg cgctcaaggt
     8941 cgccgaccgc ggctatgtgc tggaatcggg caagatcgtt cacgaggatt cgtctgaagc
     9001 gctggcgcac gatccggcgc tggaagcagc ctatctcggc aagatggaag cggttcattg
     9061 accatgggcg acgatttcat tctgcgcaac gccagggtcg ccggacgcgg aggagagatc
     9121 ttcgacatac tgagccgcga cggcgcgatt gccgagatcg cgccgcgcat cgcaactgat
     9181 gcgcgcagtg aggatgtcga cggtcggctt gtgctgggag gtttcgtcga cagccatatc
     9241 catctcgaca aatcctgcat tctcgaccgc tgccatatcc ggcacggcac gctcgaggag
     9301 gcgatcggcc aggtggcggc cgcaaaacgc gccttcaccg aggaggatgt ctacgtgcgc
     9361 gcctcccgca cgctggagaa ggcgatcctg cagggtacta cggcgatgcg cacccatgtc
     9421 gaggtcgacg cgcggatcgg cctcacaagc ttcaatgcga tcaggaagct cagggacgac
     9481 tacgcctggg cgatcgacct cgagctttgc gtgtttccgc aggaaggcat gatcaacgat
     9541 cccggcaccg aggaaatgct ggttgcggcc tgcgaggcag gcgccgacct gatcggcggc
     9601 tgtccctatg ccgacagcga ccgcgcgcgg catatcgagc acatctttgg gatcgcgcgc
     9661 cgcttcgatc tcgatatcga tttccatctc gatttcgatc tcgatccgtc ctggaccgac
     9721 atggaagaag tctgcctgca ggcggaggcc catcgctatg gcggccgggt cgcgatcggc
     9781 cacgtcacca agctgtcgtc aatgccgagc gcggaattcg agacatgggc caggcgcctc
     9841 gcagacgcgg gcgtcgcggt caccgtgctg ccgtcgaccg acctgttcct gaccgggcgc
     9901 gatcacgatc acaatattcc gcgcggcgtc gcgcatgcgc accggctttc cgcccaaggc
     9961 gtaacctgct cgcttgcgac caacaacgtg ctcaacccgt tcacgccgtt cggcgattgt
    10021 tcgctgatcc gcatcgccaa tctctacgcc aatgtcgccc agctcggaga cgggccggca
    10081 ctctgttcct gcttcgatat ggtgacgcga gggcctgcct cgctcatgaa cctcagccaa
    10141 tacggcgtcg aggtcggaaa tcccgccgat ctcgtcgttc tggattgctc ggaggaggcc
    10201 gcggcggtgg cggaaatcgt gcagccgctg gttgcatata agcggggccg gcgcagcttc
    10261 agccgggcgc agcccgtgct gcatcgaccc aattatggtt aacgcaacac tgttaatctt
    10321 acatcttaac cattgattaa ttatacgttt gcagggcgcg cgcggcccgg cctagcgtgc
    10381 cttggggggc gggttgcgca gcaacgagcg aatggtacgt gccatgacgt ataaaatgaa
    10441 tacggcgttg atcgcctctc ttagcgtggt cgcgctgatg cttgccgccg acgaccagac
    10501 ctttgcaagg tcgggagcgg gacgcggcgg ggttgtgtcg gcgcgcgcga tatctcatcc
    10561 cttccggcat cacccgagaa atttcggaaa ttttggaggt tttggggctt tctggccagg
    10621 ggactttgcc aacggatcat acggtccaaa ctacggcgag cccatgctga gtcctccgca
    10681 ggcaacgtcg agcgatatcc gttacaccca aacatacgat gttccctggg attgggccca
    10741 tcgctttccg ccgaatgtca ctccgtccga ccggccctat gtgtcgagct gcccggtgga
    10801 aactctcacc ttccatcagc atgacggggc ggacaaaacc gtcaatgtca tgcggtgcta
    10861 ctgaaacggc actggcgaca caacaaggcg tctgagagga gcagtctcga tcgaagactg
    10921 ctctccggtc gatctgcagg ccgcatcctc cgaggttgat ccactcccgc tttcgcggca
    10981 tctttgatgc ttgcgttcgc agggagatgc aaagtgaccg acggaccaat cgatggcggc
    11041 aaggcggcgc ggccgaagct cttccagcca tttgcgatcc ggggtctcac gctcaaaaat
    11101 cgcctcgtcg tgccgccgat ggttcattat cgcgccgagc ccgggaacac ctgcggcacc
    11161 ttccatctcg tgcacctcgg ccgctacgca ttaggcggtt tcggtctcgt ctttgtcgag
    11221 gcgacggcgg tggaggaggt cggcctcatc aacgagcacg atctcggcat ctggaacgag
    11281 gcacaggccg aaagcttcaa gccgctgatc gctttcatga ggcggcaaaa cactgcaatc
    11341 ggcattcagc tcgcccatgg cggccgcaag tcgtcgtcgc aaacggccat gcagggcatg
    11401 ggaccgctga ccgagga
//
LOCUS       DATLJT010001580         7648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1304964,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001580 DATLJT010000000
VERSION     DATLJT010001580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304964"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VM910_36185"
     CDS             complement(<1..1130)
                     /locus_tag="VM910_36185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67981.1"
                     /translation="MVGSNEEALLTVIDRIYESVEAPELWPETIYAVGELIGGRCHFW
                     GPDQDSSAGVARSPHELEIGCYGTFFLSRQDLQVLDEYAEEFSDLITRFLKIVFLSIL
                     RSQKEVGVREAIGLKITERYLDAVQPLSGTSTLPARLASRRLIAALWEDGRIFSGDKL
                     HYMRILAPHLERAVRLQMQWRAADLRTDILSGVLDSLAHGVVLVDHSGLPVSMNRRAK
                     EIMETSNGLRLSRTGIAGHRPCDTESIRDLINGAMSAGKQGLLAISRVDGLRPLLLIA
                     VPMKPNGGPGGSSQFAQAVVFICDPDRTDNPTVETLQRAFDLTYREAQMAIAIARGHG
                     LQAAADRMGVALTTARSQLQQAFAKTGTSHQAELAALVHRTLT"
     gene            1903..3891
                     /locus_tag="VM910_36190"
     CDS             1903..3891
                     /locus_tag="VM910_36190"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI67982.1"
                     /translation="MSARFSTSSDLDGLPLNSLIHDAFKIRGAETKLLELFAAGELNG
                     TVHTCVGQELFPIVLARYLRADDYVVSNHRGHGHYLSVTGDFEGLIAEVMGRTSGPSG
                     GYGGSQHLRTARFYSNGIQGGMTPIAVGLAQALKEESTRNLCVCFIGDGTLGEGVVYE
                     AFNLAAKWQVPVLFAIEDNGYAQSSSKRQTLAGSIAGRAAAFGIRYFEANIVDVRGLD
                     ETCGAATRYIRGELRPAIVHVHTTRLNSHSKGDDNRDCGEIQALRKRDIVNRYQEAGL
                     IDDVQASSIEQELVATVGRARSSAPLLEVNRNCPVHNTAVGFRTVVSRSERRVVEQTN
                     EALRRLLAQHSDLLFLGEDIEDGNEFTPGPYGGAFKVSRGLSHEYPGRVRNTPISEAG
                     FTGLAVGRALAGKKTVVEIMFGDFCTLIIDQLLQHADKFPTMYGTDVALPLMIRTPMG
                     GRRGYGPTHSQSIERLFMGMQNLVVLAVNHHLDIPALYSAALNGPPKPTFMIENKVLY
                     TIANTAMCPKGYAIEQSDERFPTVRIKPARHPPSFTIFTYGHGLALAEAALVQLMHDY
                     EVFGDIICPSALTPINLSPLIDSVSNTKRLLTVEEGSTFGALGAELISQLLEHGVALR
                     SIRRLGNGTILPCSAKAEARLLPSSQDIVQCIFEMTSK"
     gene            3888..4340
                     /locus_tag="VM910_36195"
     CDS             3888..4340
                     /locus_tag="VM910_36195"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HVI67983.1"
                     /translation="MSEIIEIKLDRLSVNDDEYTFVERLVEDGAEVAAGATIASVESV
                     KTVFEIEAPTAGFVFFLKAPKDQVAVGERIAVISPIQEFDKTRFSHSMDPSAPAPPVD
                     DPIYAKFTARALELAKGKRIDVEYFRDFDAVTSADVEAYLRHETSRAE"
     gene            4365..4763
                     /locus_tag="VM910_36200"
     CDS             4365..4763
                     /locus_tag="VM910_36200"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HVI67984.1"
                     /translation="MGLDLAILLMFQFAGEMMRAVTGLPVPGPVIGMVLLLVALLAKL
                     PVPAGIHATSRKLLAYLGLLFVPAGAGVVTRLPMIGAHWLPIVIAVVGSTIITMVITA
                     VVMRGLERLGTVNWRAIGFRKPSPSAEGST"
     gene            4760..5473
                     /locus_tag="VM910_36205"
     CDS             4760..5473
                     /locus_tag="VM910_36205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HVI67985.1"
                     /translation="MTLVWAQFTSSPAFALALTLGAYEIGRHLQQQFRSSPLINPVLI
                     AIIIVGCSLGAIGISYEQYFDGARLIHILLGPATVALAIPLYAHIEHVRRSTLAILVA
                     VCIGAATGAMSAIGLAWLFGAPDVIVKSLAAKSVTTPIAMALSEQFGGLPDLTAVLVI
                     ATGIFGAIVAAPLLDLIRIECWRARGIATGVAAHGIGTAQILSISELGGAFSGLGLGL
                     CGLATAALMPFINWLLWHR"
     gene            5820..6149
                     /locus_tag="VM910_36210"
     CDS             5820..6149
                     /locus_tag="VM910_36210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67986.1"
                     /translation="MSTRLSRRAALLTALVFAVPTVGPTIAEAQADASDAANLYPGRY
                     AAVCIPAPMFGCVCTTDPLGEPLMFTEIDSKANHHLKEIGDTEYLQMIAWLRRTCASL
                     APSVVPP"
     gene            complement(6616..>7648)
                     /locus_tag="VM910_36215"
     CDS             complement(6616..>7648)
                     /locus_tag="VM910_36215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI67987.1"
                     /translation="RDYPARASVGKRAGVSPAGSSNPWTAKVLGSRPASDFDNPRRRG
                     DRINRRAFITLLGGATAAWPLAARAQQSAMPVVGFLRSESPESEAFRLAAFRKGLNEA
                     GYTEGRNVAFEYRWAHGQYDRLAALASDLVRLQVSAIAAIGLTPAALAAKAATPAIPI
                     VFAVGGDPVGLGLVASLNRPGGNLTGVSFLVSMMAPKRLQILHEAMPDAAVMGFLVNP
                     NDVYAESETNEMEPAAAAIGQKLLVVKASVESELETAFAELMKQRVGALCVEADQFFV
                     HNPPTSDHSTHQGPGPAARRKDGPRPSAGLRRFATPWGWGMGRIESERISPAQFPQAI
                     KNLREPQAD"
BASE COUNT         1602 a         2227 c         2202 g         1617 t
ORIGIN      
        1 gtcagcgtac gatgcacgag agccgcgagc tccgcttggt gacttgtgcc cgtctttgcg
       61 aaggcttgct gaagttgact tcgggcggtg gtcagagcga cccccatcct atccgcggcc
      121 gcctgtagac cgtggccacg cgcgatcgct atcgccattt gcgcttcacg ataggtcagg
      181 tcgaaagctc gctgtagagt ttcgactgtc ggattgtcgg tccgatccgg atcgcagatg
      241 aaaacgacgg cttgcgcgaa ttgacttgag ccgccaggac cgccgttcgg cttcattgga
      301 acagcgatta gcaagagagg tcttaaacca tcgactcggc tgattgcaag caagccctgc
      361 tttccggcag acatcgctcc gttgatcaag tcgcgtatcg actcggtatc gcacggccgg
      421 tggccagcaa tgccggtacg cgacagccgt aagccatttg aggtttccat gatctcctta
      481 gctcgcctat tcatcgacac aggcagccca gagtgatcta ccaaaacgac accatgcgca
      541 agcgaatcga gaacaccaga taatatgtca gtccggagat cagctgcgcg ccactgcatc
      601 tgcaagcgca cggcacgctc gagatgcggc gcaaggatgc gcatataatg cagcttatca
      661 ccgctaaaga tccgtccatc ctcccaaagt gctgcaatca atcttcggga cgccagccgc
      721 gctggtaatg ttgatgttcc actcaacggt tggactgcgt ccagatagcg ctcggttatc
      781 ttcaagccga ttgcctcacg aacgcccacc tccttttgag accgaaggat gctcaggaac
      841 acaattttga ggaaacgagt gatcaggtca gagaattcct ccgcgtattc atcgagaacc
      901 tgcaggtcct gacgagaaag aaagaaggtt ccatagcagc caatctcaag ctcatgcgga
      961 cttcgcgcaa cgccggctga gctatcctgg tctggccccc agaagtggca tcgtccgccg
     1021 ataagctcgc caaccgcata gatggtctct ggccaaagct ccggcgcttc gaccgattca
     1081 taaatgcgat caataaccgt cagcaatgct tcttcgttgg agcccaccat tggcgcagcc
     1141 ctgactaagg aggtttcgcc acttggagcc tagctgaaag tatttcagaa tttttcatcc
     1201 gttcatagga agagcgacgg ccgcgggcct agtaatttcg atcaagacgg ccggcaggac
     1261 tttgccgggg aagaagcgcc gggagctaaa gatcccggcc gtgcggcctt cattgtccta
     1321 acacgcaccc aagttcgggc ggccgcgttt ttcgccaagt cggattcgaa aatcgccaac
     1381 cttggaaggc aacgtcaaga ggagaatccc cgatgagcac ggatcgccag tcggccagca
     1441 cgggacttcg cttttgaccg cacgctcttt cgcctttgtc cacgtcgccc ttgcttactg
     1501 tcactctcga tccgtttcca tcgaatctcc gaacctggtg gatcaggaat caggcagtgc
     1561 tgaaccatag tccatagcta tcgataagag gccaattcat gactttgcgc tccggtcgtg
     1621 ccttgagcag agcaatgcaa tttctctctt gatcgtgtga gccgcttcat agatcgcatc
     1681 gtaaatgcgt gcatgacttc gtttaatcca tcgagtgtac tcgattttgg ataggtgttc
     1741 ggtgggagga tggtaatgac ctcgcaagct ccgcaatgtg acgctcgaga ggaagagacg
     1801 ctcctggcta cgaacgatac cgcagctgca tccatcagcg tggacgggga acgctggcaa
     1861 caatccaccg tttgggcggg catgaagtgg ctggatcgtg caatgtctgc gcgcttttcg
     1921 acgagcagcg acctcgacgg cttgcctctc aattcgctta ttcacgacgc cttcaagatc
     1981 cgtggcgcgg agacaaagct gcttgagttg tttgcagccg gcgagctcaa cgggacggtt
     2041 cacacttgtg tcggccagga attgttcccg atcgttctcg cccgatattt gcgtgccgac
     2101 gactatgtcg tgtccaatca tcgcggacac ggacattatc tgagcgtgac cggcgatttt
     2161 gagggcttga tcgccgaggt gatggggcgg acaagcggtc cgtcgggagg atatggcggc
     2221 agccaacacc tcaggaccgc acggttctac tcgaatggaa ttcagggtgg catgactccg
     2281 attgcggtcg gccttgccca ggcgctcaaa gaggaaagca ctcggaacct atgcgtgtgt
     2341 ttcatcggcg acggcacgct cggcgagggt gtcgtctatg aagcattcaa cttggctgca
     2401 aagtggcagg tcccggtcct tttcgccatt gaagacaacg gatatgcgca gtcgtcaagc
     2461 aaacgacaga cgcttgcggg aagtattgcg ggccgcgccg cagcttttgg tatcagatat
     2521 ttcgaagcaa acattgtcga cgtccgtggt ctcgacgaga cgtgtggtgc agcgacccgc
     2581 tacatccgcg gcgagttgcg gccagcgatc gtacacgtgc atacgacgcg actgaactct
     2641 cactctaaag gagacgacaa ccgcgattgt ggtgaaattc aagcacttcg taaacgtgac
     2701 atcgtcaatc gctaccaaga ggccggtctc atcgacgatg tccaggcatc gagcatcgaa
     2761 caggagctgg tcgcgactgt cgggcgggcg aggtcatcgg ctccgctcct cgaggtcaac
     2821 cgcaattgcc ctgttcacaa cactgcggtc gggtttagga ccgtggtgag ccgttcggag
     2881 cgaagagtcg tcgaacagac gaatgaggca ttacgccgct tgctcgctca acacagcgat
     2941 ctcctctttc tcggcgaaga catcgaagat ggcaacgaat tcaccccagg gccctacggc
     3001 ggtgccttca aggtgtcgcg cgggctgtcg cacgagtatc cggggcgcgt gcgcaacacg
     3061 ccgatcagtg aagccggttt caccggtctc gccgtcggtc gggcgcttgc cggcaagaag
     3121 accgtagtcg aaatcatgtt cggcgatttc tgcacgttga tcatcgacca gctgctgcag
     3181 cacgccgata agttccccac gatgtatggg acggacgtcg cgctcccgtt gatgatcaga
     3241 accccgatgg gaggacgacg tggatacggc ccaacccact cgcaatcgat tgagcggctc
     3301 ttcatgggaa tgcagaacct cgttgtcctg gcggtcaatc accacctcga cattccggcg
     3361 ctctattctg ctgcactcaa tggtccgcct aagccgacct tcatgatcga aaacaaggtc
     3421 ctttatacca tcgccaatac ggcgatgtgc ccgaaaggct atgcaatcga gcagtcggac
     3481 gagcgcttcc caaccgtccg cattaaaccg gcacggcacc cgccgagctt caccattttc
     3541 acgtatggcc atggcttagc acttgctgaa gcggcgctgg tgcagctcat gcacgattac
     3601 gaagtatttg gggacatcat atgtccctcg gcgctcacac ccatcaatct ctccccgctg
     3661 atagactcgg tgtcgaatac aaagcgcctg ctgactgtcg aggaaggctc gacgtttggc
     3721 gcactcgggg cagaactcat ctctcagctg ctcgaacatg gcgtcgcgct gcggagtatc
     3781 cgcaggctgg ggaatggcac gattctgccg tgttcggcaa aggcggaagc gcgtctgctg
     3841 ccatcatcgc aagacattgt tcaatgcatc ttcgagatga cgagcaaatg agcgagatca
     3901 tcgagatcaa gctagaccgc ctgtcggtca acgacgacga gtacacattt gttgaacggc
     3961 tggtggagga cggggcagaa gttgccgccg gcgcaaccat tgcaagcgtt gaaagcgtca
     4021 agactgtatt tgagatcgag gcgccgactg ctggctttgt cttcttctta aaagcaccga
     4081 aggaccaggt ggctgtcggg gaacgcatcg cggtgatttc tccaatccag gaattcgaca
     4141 agacgcgatt ttcccactca atggatccca gcgcgcctgc gcctccggtc gacgacccca
     4201 tttatgcaaa attcacggcc cgcgcgctgg agctcgcgaa gggcaagaga atcgacgttg
     4261 aatatttccg ggatttcgac gccgtcacgt cagccgatgt ggaagcgtac ctgcgtcacg
     4321 agacctcacg cgcggagtga gaacgccgac agcggagcca ggagatgggt ctggatctgg
     4381 caatattgct gatgtttcag ttcgccgggg aaatgatgcg ggccgtcact ggcctgccgg
     4441 taccaggtcc ggtcatcggg atggtcctgt tgctcgtcgc cttgctggcg aagttgcctg
     4501 tgccggcagg cattcacgca acgtcccgca aactcctggc atatctcggc ctgctttttg
     4561 tgccggccgg cgccggcgtt gtgactcgct taccgatgat cggcgcgcat tggctcccga
     4621 tcgtaatcgc cgtcgtggga tcgacgatca tcaccatggt gataaccgcg gtggtgatgc
     4681 gcggattgga acgccttggg acggtcaact ggcgcgcaat aggatttcgc aaaccatccc
     4741 caagcgcgga agggtcgaca tgactcttgt ttgggcacaa tttacctcgt cgccggcgtt
     4801 cgcgctcgct ctgacgcttg gggcatatga aatcggccgt cacctacagc agcagtttcg
     4861 ttcatcgccg ctgatcaatc cggtcctgat tgcaatcatc atcgtcggat gttcccttgg
     4921 cgcgatcggg atatcttacg aacaatattt tgatggcgct cgtctgattc atatcctgct
     4981 cggcccggct actgtcgcgt tggccatacc gctttacgct catatcgagc acgtccggcg
     5041 atcgacactc gcaatcctag ttgcggtatg catcggtgcc gccaccgggg ctatgagtgc
     5101 gatcggactg gcctggctat tcggcgctcc ggacgtcatc gtgaaatcac ttgcggcaaa
     5161 atcagtgacg acgccgatcg ccatggcgct gagcgaacaa ttcggcgggt tgcccgactt
     5221 gaccgcggtc cttgtcatcg cgactggcat ctttggcgcc atcgtcgcgg cgccgctgtt
     5281 agatctcatc aggatcgaat gctggcgcgc acgcgggatc gcaaccggtg tcgcggcgca
     5341 cggcatcggt accgcgcaaa ttctctccat cagcgaactt ggcggggcat tttcgggcct
     5401 cggcttgggg ctttgtggtc ttgcgaccgc agcgctcatg ccgttcataa actggttgtt
     5461 gtggcatcgc tgagaggatt gcagttcggc atagcgacaa acgcgagaca cgcaagacgt
     5521 tgcacatgca gtacgacgaa cgatttcggg catgaatgat gtacgccctg tgtgtgtatc
     5581 gaacactcct gactcggacg tagttagcgt cctgtccggt gaacgggcga gagcctgcgc
     5641 agcagtgtgt gatcgatctc acatactagt cccagagtcg ctgttagatg gaatgccacg
     5701 aaacaaaact tgctcacagc agactccagt cagtctctgg ccgaaagcga cattcgaatg
     5761 aggcgagcca aacggaggaa tcggtgttca agatgttgtg cgccggagga ccgatctcga
     5821 tgtcgacgcg cctgtcgcgt cgggccgccc tgttgaccgc actggttttt gccgtaccga
     5881 cagttggacc aaccatcgcg gaggctcagg cggacgcgtc tgatgcggct aacctctatc
     5941 ccggtcgtta cgccgctgtc tgtattcccg caccaatgtt cggctgcgtg tgcaccacag
     6001 atccgttggg agagccattg atgtttactg aaatcgacag caaggccaat catcatctca
     6061 aggagatagg ggataccgaa tatttgcaga tgatcgcgtg gctgcgacgg acttgcgcct
     6121 cgctcgctcc atcggtggtt cctccgtgag ccggtactgc tcgtaggtcc atccaccaac
     6181 ctcatcttcg cagaacgtcg agtgcgcggc aacgcgacaa cagagaaagt cttgcagaaa
     6241 aacgtcgccg gacattgaaa caaatcgcga gagacgacaa tgacaatgca tgcttccttt
     6301 tcaatcttga atccccccgc ccatacagaa cgccgatcag ttctaaggca gtttattgac
     6361 gctgtgatcg aaggtcgccg atgcaaggca gcgcacgagc ttgcgttata ccttgagcac
     6421 cacaagcaca gtctaaaaga cgacgtcagg attgaactcc agaatcggct caggggtcga
     6481 tagagagacc cagatctcgc ggtcattcca tgcctgacat tcgtccagaa accattagcc
     6541 gcaaggaggg cgcgtataca agagaatgaa ggattcgcca ttatttggcg tgaggctcag
     6601 cgcaaacgtg gcgaattaat cggcttgtgg ttctctcaga tttttaatcg cctgtggaaa
     6661 ctgcgccggg ctaattcgtt cgctctcgat ccgtcccatc ccccatcccc acggcgtggc
     6721 gaacctacgc aagccggctg acggccgagg cccgtctttg cgcctagccg ctggacctgg
     6781 cccctgatgg gtgctgtggt cgctggttgg aggattgtgt acaaagaatt gatcggcctc
     6841 cacgcaaagg gcacctaccc gttgtttcat tagctcggcg aacgctgtct ccaactcgct
     6901 ctcgacgctc gctttcacga ccagcagttt ctgcccgatg gccgcagccg ccggctccat
     6961 ttcgttcgtc tcggactcgg catacacgtc gttcggattt actaggaagc ccatcactgc
     7021 tgcgtccggc atcgcctcgt gcaggatctg caatcgcttt ggcgccatca tgctgaccag
     7081 gaagctcacg ccggtgaggt tgccgcctgg ccggttgaga ctggcgacaa gccctaaccc
     7141 gaccggatcg ccgccgacgg caaacacgat ggggatcgcc ggagtcgccg ccttcgccgc
     7201 gagcgcggcc ggggttaggc cgatcgcggc tattgcggaa acctggaggc gaaccagatc
     7261 ggaagctaat gcggccagtc ggtcatactg tccgtgtgcc caccgatatt cgaacgccac
     7321 gttccggccc tcggtatagc cggcttcatt caggcccttc cgaaacgcgg ccaagcgaaa
     7381 cgcttcggat tccggcgact cgctgcggag gaacccgacc accggcatcg ccgactgctg
     7441 cgcgcgcgcc gcgagcggcc acgcggccgt cgcgccgccg agcagcgtga tgaacgcgcg
     7501 gcggtttatt cgatcgcctc gtcggcgcgg gttatcaaaa tcgctggcag gtcgagagcc
     7561 gagcaccttg gcggtccagg ggttcgatga gcccgcgggg ctcacgccgg cacgtttgcc
     7621 cacagaagca cgagcaggat agtcgcga
//
LOCUS       DATLJT010001581         5585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_616258,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001581 DATLJT010000000
VERSION     DATLJT010001581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616258"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VM910_36220"
     CDS             complement(<1..199)
                     /locus_tag="VM910_36220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67988.1"
                     /translation="MTRRHNSALVAVLACASLLAVSHYAAARTTHGGKGSSVATAKAA
                     GKTAKSTGKKAAARKSKDDKSA"
     gene            complement(981..1298)
                     /locus_tag="VM910_36225"
     CDS             complement(981..1298)
                     /locus_tag="VM910_36225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67989.1"
                     /translation="MSELVQFLCKGDHPVEASLREKTRAALKESIAGGHVRIKFTDTR
                     GGTELGVPIDRSRSDLRAIESDNGSAEIRVVGDLTLDYVKVTCVARIDLATLQGEGHL
                     EVR"
     gene            complement(1518..1739)
                     /locus_tag="VM910_36230"
     CDS             complement(1518..1739)
                     /locus_tag="VM910_36230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67990.1"
                     /translation="MSFVVVSIYLAAVVFCVGTTAWILRAWTRRRYWHKSPSDLHYVV
                     AGTALLLSAGIGVAVASQIVVALGYPPAP"
     gene            complement(1822..2532)
                     /locus_tag="VM910_36235"
     CDS             complement(1822..2532)
                     /locus_tag="VM910_36235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI67991.1"
                     /translation="MTDIDIIRVQRLDFLVSPLAWSFAADRRTEIEDYFTHLRRGKPQ
                     LWNGRVLLLRDFEISDTTLQGTMFETDYASFIAWRDWGFPDRRVRACFAMGALLSRDG
                     AFLLGVMNAHTAGAGGIYFPAGVPDPRDVVHGRVDFDRNVRREIAEETGLSLEGIAAD
                     PWLLVLAQQRVALIKVFRFRKSAADMRASILDHLQKEALPELSDVHIVRDPSDFKPSM
                     PPFVRAFLLHFWDCDSER"
     gene            2819..3016
                     /locus_tag="VM910_36240"
     CDS             2819..3016
                     /locus_tag="VM910_36240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67992.1"
                     /translation="MIWRVEKVENIDMGLGFIPSPGFTIQQDGRSPSLTIIFEDLKTA
                     EQCASSMREIIDKATAIRDRD"
     gene            3295..3636
                     /locus_tag="VM910_36245"
     CDS             3295..3636
                     /locus_tag="VM910_36245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67993.1"
                     /translation="MSAKPQQLGPPNIGTPASTIPLGLRVLAMALRALFIGALVVVTV
                     RVSSPQSETFSSVYETPGDLIRLALGFAVCLWIVIHLFMLPKDAEGYRTWVYLGLVVV
                     PLAVAVAFVLW"
     gene            complement(3757..4872)
                     /locus_tag="VM910_36250"
     CDS             complement(3757..4872)
                     /locus_tag="VM910_36250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI67994.1"
                     /translation="MRPLEGLFVLDFSTLLPGPLATLLLAEAGAEVVKIEPPRGEEMR
                     GRSPKWGHDSAGFALLNRGKRSVAIDLKNTAERARLQPLVERADVIVEQFRPGVMARL
                     GLDYVTVAKLNPAVVYCSISGYGQTGLKRDVAAHDLNYISDAGLLALSMGDPSRPVLP
                     PTLIADIAGGAYPAVVNILLALEERRRTGRGRHLDVAMAENLFPLMFWAIGHGLVAGT
                     WPGNGAGYVTGGSPRYGLYPTKDGRVVAAAPLEQKFWETFCDLIGLEGEFRDDGENPA
                     ATKARIGAIIASETADIWRSRFAGKDCCCCIVANLGDALTDPHFMARGVFHHVLANPE
                     GAGMPALPVPLDPAFRAAPAAAIAAPALGADNKRYLA"
     gene            5034..>5585
                     /locus_tag="VM910_36255"
     CDS             5034..>5585
                     /locus_tag="VM910_36255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI67995.1"
                     /translation="MRTRSNGDGMSKDNVTVGFVGLGTMGGRMAANVQRAGFRLLVHD
                     LHRQAASHHLADGAVWAESPCALAREADVIFSSLPEPSDVEAVALGPDGLVAGIKPGA
                     AYFDLSTNSPSVVKKLNAAFAERGAHMLDAPVSGGPAGAASRKLAIWVGGDENVFNRH
                     KAVLDAIGDKAAYIGPIGSATVAK"
BASE COUNT         1067 a         1793 c         1724 g         1001 t
ORIGIN      
        1 aggccgattt atcgtccttg gacttgcgcg ccgccgcctt ctttccggtg gacttcgcgg
       61 tcttacccgc ggctttcgcc gtcgcgacgg acgagccttt gccgccgtgc gtggtcctgg
      121 ccgcggcgta atggctaacg gcgagcagac tcgcgcaagc gagcacagca acgagtgctg
      181 agttgtgtcg tcgtgtcact tttgctgatc cccaatcccc agcggattac tgcgctcccc
      241 agcacggtaa tcacgcgccc cgatttgacg gcgcctatgc tacccacgat ttgggcggaa
      301 aagcgatgtc gagatggcaa ttcatggcct caagcccatg gcacaagcgc gatttatacc
      361 cgcaaatgct cgtcaatcca gcgtttgccg tctgacctca atacccgtcc gtgagcggac
      421 gacgcaggtt ctcagaagtg ctattgcgcg tctcgtgtta gcgtgtgcga gccaaccgag
      481 tgcggtccgg cacctagttg gtggcggacg cgacaggccc tagccggaaa gccccatgcc
      541 ccacgtgagg cggcgtgagt tcgtcctgct gctggcggcg cggcggacgt ccgcgcgctc
      601 gtggcgcgcg cgacggtccc tgcgcgacga tcgcggggtg gctgcagcca tgaatcgttg
      661 gcggccgacc gctgcgctcg cggccatggt cctgttcggc agtttgctgg cgtggggagc
      721 cgaggcccgc atcctggtat gctggtggga atatccgggc cggatgtgca cgatcgttaa
      781 gtcggccaag tccaccctac gaccacagtg ggtcggcaac ggtcatcgca gagcgccgcc
      841 cctatgcccg cgcggatttt gggccgtctg tggcgagtgc ccctgctagg cttcccacgc
      901 gaggcaacga tgtcagccat tgggatccgc gctgggggct gaagaacaac cgccctcgcg
      961 ccgcgccgga attcatgctc ctaacgcact tcgagatggc cctcaccctg gagcgtcgcg
     1021 agatcgatgc gggcgacaca ggtgactttc acatagtcga gcgtcaggtc tcccacgact
     1081 ctgatctcgg cagacccatt gtcactttcg atcgcgcgca agtcggagcg gctcctgtca
     1141 ataggcacgc cgagctcggt gcctcctcgc gtgtcggtga atttgatgcg gacatgacca
     1201 ccggcgatgg actccttcaa ggccgcgcgc gttttttcgc gcaggctcgc ctccaccgga
     1261 tggtcgcctt tgcagagaaa ttgcaccagc tcgctcactc cgctcctcct cgtcgatcga
     1321 tcgttttcgc ggctaccgtt tggaactgct tgcgcccgtt gatgtcaagc cgaccaggcc
     1381 atggcgcgtg gcgattgggg ggtcaagagc aacagatttc aagagcgaac aaaaattgaa
     1441 cgcagatcga tgggcgacgc ggattgacgg ccaccgaagc tgcccacggg atttgcaacg
     1501 aaggcaactc aggcggctca aggtgctggc gggtaaccga gcgccacgac gatttgtgac
     1561 gcgaccgcga ctccaattcc cgcgctcagc agcagtgccg tacctgccac cacgtagtgc
     1621 aggtcgctcg ggctcttatg ccagtaccgc ctgcgggtcc aagctcgcag gatccacgcc
     1681 gtcgtcccca cacagaagac aacggccgcg agatagatcg aaacaaccac gaagctcatg
     1741 ctgcgcagac cgagcgaatc tcgtcagatg gtacaccgca gcgaaagttg cgaccatgcc
     1801 gtcgcctggg cgaaacggac gtcatcgctc gctgtcgcaa tcccaaaaat gcagaaggaa
     1861 cgccctgacg aatgggggca tgctcggctt gaaatcggaa ggatcgcgaa caatgtgaac
     1921 gtcggaaagc tcaggtaggg cttctttttg cagatgatcg agaatactcg cgcgcatgtc
     1981 cgccgccgac ttgcggaagc ggaatacctt gatgagcgcg acccgctgtt gtgcgagcac
     2041 aaggagccag ggatcagctg caataccctc aaggctgaga ccggtctcct cggcaatctc
     2101 ccgccgaacg ttgcgatcga aatcgacacg gccgtggacg acatctctcg ggtcgggcac
     2161 gccggccgga aagtaaatcc ccccggcacc ggcagtatgg gcattcatga cgcccaacaa
     2221 gaatgcgcca tcgcgggata gcaaggcccc catcgcgaag cacgcccgga cgcggcggtc
     2281 gggaaagccc cagtcgcgcc aagcgatgaa acttgcgtag tcagtctcga acatagtgcc
     2341 ctgcagcgtg gtgtcggaga tctcaaaatc gcgcaggagc aggacacgcc cgttccacaa
     2401 ttggggcttt ccgcgccgca aatgcgtgaa atagtcttcg atctccgtgc gccggtccgc
     2461 agcgaacgac catgccaggg gagaaacaag aaaatccaag cgctgaacgc gaatgatatc
     2521 gatgtccgtc attttgcatg ttcggcgccg tgtggcgctc cgcagaaatt tcgattccag
     2581 cttggtcagc gcccgctcga agacccctac ggtgccggtg ccctgtcgcc gtccttctcg
     2641 cctgacgtcc ttcgctctcc gtcgaggaaa gaaattcgat cttatcgcaa ttgaccgaaa
     2701 aacgtgatcg atgccagtct cctgctgcga cgatcatccc gtcggctacg gatgcggccc
     2761 acggcgtctg ccttagctcg aatcggccca gggacgttcg acgcgaggga gcaatcacat
     2821 gatatggaga gtcgagaagg tcgagaacat cgacatggga ttgggcttta ttcccagtcc
     2881 cggattcacg attcaacaag acggccgatc accctcgctg acgataatct tcgaggacct
     2941 gaagactgcc gaacaatgcg cgtcctcgat gcgcgaaatc atcgacaagg cgacggcgat
     3001 cagggaccgc gattaacggc tctccccagg cgctgcgtcc aggacgcgga cccgagacgg
     3061 acgtccctcc taagccggat gtcagcggcc gagcgcgggg ccgatcagca cggcaagggc
     3121 gccgcgtatt tgatcaaacc gctcttttgc acgccacggc gcggtgcccg ggtagtcgtc
     3181 agcaatgccg ggaggatgcc cctgtcttgc gccgcggcat tgcggagggc gcgacggacc
     3241 ttacataagg ccctgagccg gaaacggagg gcagggatcc gccagattga cttaatgagc
     3301 gccaaaccgc agcagctcgg tccgcccaac atcgggacgc cggcatcgac catcccgctg
     3361 ggactgcggg tattggctat ggctttgcgt gcgctgttta tcggggcgct cgtggtcgtg
     3421 accgtgcgcg tgagcagccc ccagagcgag accttttcgt ccgtttacga gacccccggc
     3481 gatctcatcc ggctcgcgct gggatttgcg gtctgtctat ggatcgtgat ccatcttttc
     3541 atgcttccca aggacgccga gggatatcgc acctgggtct acctcggcct tgtggtcgtg
     3601 ccgctcgcag tcgcggtcgc cttcgtcctc tggtgatcgc accacgacac gcgcgtccct
     3661 ttcgaggaac aaagagcggt cacgcagcgc ggacctcgct gcaatgacta ggtttggccc
     3721 agaaccgaac ccgcgcgtgc tgcgtgctgc gacccatcag gctaaatacc tcttgttatc
     3781 ggcgcccagt gcaggcgcgg cgattgctgc tgcgggagct gcgcgaaagg ctgggtcgag
     3841 cggcaccgga agcgccggca tgccggcacc ctcgggattg gcgagcacgt ggtgaaacac
     3901 gccacgcgcc atgaagtgcg ggtccgtcaa tgcatcaccg aggttcgcaa cgatgcagca
     3961 gcaacaatct ttgccggcga accgcgaacg ccagatgtct gcggtctcgc tcgcaatgat
     4021 cgcgccgata cgcgctttcg tcgccgccgg attttcccca tcgtcacgaa actcgccctc
     4081 gagaccgata aggtcgcaga acgtctccca gaatttctgc tcgagcggcg cagccgctac
     4141 cactctgccg tccttggtgg gatagagacc atagcgaggc gagccgccgg tcacgtagcc
     4201 cgctccattt cccggccaag tgccagcaac gagaccatgg ccgatcgccc agaacatcag
     4261 cgggaacaga ttttccgcca tcgccacatc gaggtggcgc ccgcgtcccg tccggcgacg
     4321 ctcctctaac gccaatagaa tgttcaccac agccgggtat gcgccccccg cgatgtccgc
     4381 gatcagcgtc ggcggcagga ccggccgcga cggatcgccc atcgacaatg ccagcagacc
     4441 tgcgtcgctg atatagttga gatcatgggc ggcaacgtcg cgcttgagac cggtttgccc
     4501 atatcccgag atcgaacagt agacgaccgc gggattgagc ttggccaccg tcacgtagtc
     4561 gagcccaagc cgcgccatga cgcccggccg aaattgctcg acgatgacat ccgcgcgttc
     4621 gaccaggggc tgcagtcggg cccgttcagc agtgtttttg aggtcgatgg cgacgctgcg
     4681 cttcccgcga ttgagcagcg cgaagccagc gctgtcgtgc ccccatttgg gggaacggcc
     4741 gcgcatctcc tcgccgcgcg gcggctcgat cttgaccacc tccgcgcccg cctcggccaa
     4801 cagcagcgtc gccagcggcc ccggcagcaa ggtcgagaag tcgaggacga acagaccttc
     4861 aagcggccgc atcgcacgtt ccttgttctg gagagagggg cgatatttat cgtgcagatc
     4921 aattcgcttt ggaagggccg gcgcaagccc gctcttgaag cgagcatctc gcgttctgcg
     4981 ctgggcttcc gcaacctgag gcacacggct agcctgtacc gtaccgatcg gaaatcagaa
     5041 ctcgcagcaa cggtgatggg atgtccaagg acaacgtgac ggtggggttc gtcgggctgg
     5101 gcaccatggg cggccgcatg gccgccaacg tgcaaagagc cggcttcagg ctcctcgtcc
     5161 acgacctgca taggcaggcg gccagccatc atcttgccga tggcgccgtc tgggcggaga
     5221 gcccttgcgc cctcgccagg gaggccgatg tcatcttctc ctcgttgccg gaaccgagcg
     5281 atgtggaggc ggtcgcgctc ggaccggacg gcctcgtcgc cggcatcaag cccggcgccg
     5341 cctatttcga cctgtcgacc aattcaccca gcgtcgtgaa gaagctcaac gctgccttcg
     5401 ccgagagagg tgcgcacatg ctggacgcgc ccgtgagcgg cggcccggcg ggcgcagcat
     5461 cccgcaagct tgcgatctgg gtcggcgggg acgagaacgt cttcaaccgg cacaaggccg
     5521 tgctcgacgc gatcggcgac aaggccgcct atatcggacc gatcggcagc gccaccgtgg
     5581 ccaag
//
LOCUS       DATLJT010001582         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_842012,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001582 DATLJT010000000
VERSION     DATLJT010001582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842012"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..875
                     /locus_tag="VM910_36260"
     CDS             174..875
                     /locus_tag="VM910_36260"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI67996.1"
                     /translation="MRAIDVMTSHVVTATPQMTVQDVAKLMINNRISGIPIVDGDRQL
                     VGIVTEGDLLRRAETGTERQRSRWSEWFSSNSRLVAEYIKSHSGRLADVMTRDVVGVS
                     ELATLGEIADLMETRRVKRVPVLHDRKLVGIVSRADLLRVLVSSGANSPDDDRDGAIR
                     SRLLADLRKQRWVNCNESDIVVSDGVVHFWGVVGSEEERTALRVAAENIPGVRGVEDH
                     TISGPLRPGPLFPVI"
     gene            complement(1133..1708)
                     /locus_tag="VM910_36265"
     CDS             complement(1133..1708)
                     /locus_tag="VM910_36265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67997.1"
                     /translation="MHGKLATVIDRIEHGHHVFRAYFKHAFLKQYEKFATFLRNELVS
                     NNLADFHLKKGLVKIHDPRVIRLLEILLHGGSQVGGWTAKQINHAVLTTFQLSDRTYG
                     LNQLRYDLRKLKAHGLLQRDGSCYAYRLTPKGVHVALLFLFFHKRLCGPLANSRFHHC
                     PDTHHKPASNLEAAYHRADKAIQQIVDLLAA"
     gene            1661..1870
                     /locus_tag="VM910_36270"
     CDS             1661..1870
                     /locus_tag="VM910_36270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI67998.1"
                     /translation="MAVLDAVDDGGQLAVHRAVQARAKDLGNLVGGQPPQAEFAAAFE
                     QLVDGKVALEDEVAAILDLSDRIEA"
     gene            complement(2104..2913)
                     /gene="istB"
                     /locus_tag="VM910_36275"
     CDS             complement(2104..2913)
                     /gene="istB"
                     /locus_tag="VM910_36275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVI67999.1"
                     /translation="MSELQHQRIVELCQELRLSAMPNLYSGIAQSAAAKAASFADFLE
                     ETLRAERAARSARAREMFARVAGFPTIKTLDGFDFGFATGAPRPQIYELAGLAFIERA
                     ENVVFLGPSGVGKTHLAIALGYLATQHGHKVRFTTAADLVMTLETAQRQGRWKEALHR
                     TVSVYKLLIIDEIGYLPLGREQANLFFQVVAKRYEKGATILTSNLTFGSWDQAFAGDP
                     VLTAAMLDRLLHHSTVVSIQGESYRLKDKRRAGLLKTPAAASTWLAPVGPQ"
     gene            complement(3015..3156)
                     /locus_tag="VM910_36280"
                     /pseudo
     CDS             complement(3015..3156)
                     /locus_tag="VM910_36280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042551150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          613 a          908 c         1042 g          593 t
ORIGIN      
        1 gcgttcaacg ccgagctgaa cagcgaggat gtgcggcccg gacgaggccc gggttccggg
       61 tggattgatt ccagccgagt cgcctgtctc cagccaccaa ccaatccttg atccacatca
      121 atgacggtgc ggttttcgag actagcctcg ccggccgcac gaaggagagt ctcatgaggg
      181 cgatcgacgt aatgaccagc catgtcgtga cggccacgcc ccaaatgacc gtccaagacg
      241 tagcaaagct catgatcaac aatcgcatca gcggcatccc catcgttgac ggggatcggc
      301 agctcgtcgg gatcgtcacc gaaggcgact tgttgcgccg ggccgaaacc ggcaccgagc
      361 gccagcgctc acgctggagc gagtggttct cgtcaaattc ccgtttggtc gccgaataca
      421 tcaagtcaca ctccggacgg ttagcggacg ttatgacgcg cgacgtggtc ggcgtcagcg
      481 aattggcgac tctcggcgaa atcgccgatc tgatggagac gagacgcgtc aagcgcgtcc
      541 cggtcctgca cgaccgcaag ctcgtcggga tcgtcagccg cgccgatctc ttgcgagtat
      601 tggtcagcag cggagccaac tcacccgacg atgaccgaga cggcgcaata cgcagccgct
      661 tgctagcaga tctgcgcaag cagagatggg taaattgtaa tgaatcggac attgtggtct
      721 cggacggcgt cgtgcacttc tggggcgttg tcggatcgga ggaggaacgg acggccctgc
      781 gcgtcgccgc cgaaaatatc cccggtgtcc gaggcgtcga ggaccatacg atctcgggac
      841 cgctccgccc gggaccactg tttccggtaa tttgacctcc agcacagtgt gcagaactag
      901 agagtgtact catggtgtgg aggacaccat ggtcctggtg cactcgacaa ccaagggctt
      961 gacggcgatt gcccgcgtgc cgcaactcag tcgaccaacc ttgaagccga ctacttgcct
     1021 tcatccagtt gtaccatcag ggcccttgtc tgcgcgttaa tgagtccaag ctctctagat
     1081 tctagctcgg agaatcgtcg acaaaaagag ttcaacaatt gccgcggaaa ggtcaggcgg
     1141 cgaggagatc gacgatctgt tgaatggctt tgtcggcgcg atggtaggcg gcttcgaggt
     1201 tgctggccgg cttgtgatgg gtgtcagggc agtggtgaaa gcggctgttg gcgagcgggc
     1261 cgcagagccg tttgtgaaag aacaggaaga gcagggcgac gtgaacccct tttggggtga
     1321 gacgataggc gtagcaggat ccgtcacgct gcagcagccc gtgggctttg agcttgcgca
     1381 gatcgtagcg gagttggttg agcccgtagg tgcggtccga gagttggaag gtggttaaga
     1441 cggcgtggtt gatctgtttg gcggtccagc cgccgacctg actaccgccg tgcaagagga
     1501 tctcgaggag gcggatgacg cgggggtcgt ggatcttgac caatcccttc ttgagatgga
     1561 aatcggcgag gttgttggac accagctcgt tgcgcaggaa ggtggcgaac ttttcgtact
     1621 gctttagaaa cgcgtgtttg aagtaggcgc ggaagacgtg atggccgtgc tcgatgcggt
     1681 cgatgacggt ggccagcttg ccgtgcatcg tgcggttcag gcgcgtgcca aagatctcgg
     1741 caatcttgtc ggcggtcagc cgccacaggc cgaattcgca gccgcgttcg aacagcttgt
     1801 ggatgggaaa gttgcgcttg aagatgaagt tgcggcaata ctcgatctga gcgatcgcat
     1861 agaagcgtga gagattgagc tgctggcgct ccttggcgga gaacttaggc cccaggatca
     1921 gggtccagta gtcgaggcgt ttgcggtgtc aacgccggtc gaatcctgac ccactttgcc
     1981 ggatttcgcc gattgaaact tgacccagtc tcgttgctcg ggggcttgtc ccggtagtcc
     2041 atcggaggga cccgcgcgta gcgaagtgac ggccacgagt ctgacgcagc ggagcgcggg
     2101 aggtcactgt ggacccaccg gggcaagcca tgttgatgcc gctgccggag ttttcagcaa
     2161 gccagcgcga cgcttgtcct tgaggcgata gctctcgccc tggatgctga ccacggtcga
     2221 atgatgcagc agccggtcga gcatcgccgc agtcaacacc gggtcgccgg cgaaggcctg
     2281 atcccacgaa ccgaaggtca ggtttgaggt caggatcgtc gctcccttct cgtaacgctt
     2341 ggcgacgacc tggaagaaca gattggcctg ttcgcgcccc agcggcagat agccgatctc
     2401 atcgatgatc aacagcttgt agacactgac ggtgcggtgc agcgcctctt tccaacggcc
     2461 ctgtcgctgc gccgtctcga gcgtcatgac cagatcggcg gcggtggtga agcgcacctt
     2521 gtggccgtgc tgggtggcca ggtagccgag tgcgatcgcc aagtgcgtct tgccgacacc
     2581 ggatggaccg aggaagacga cgttctcggc gcgctcgata aaggccaggc cggcgagctc
     2641 gtaaatctgg gggcgcggcg cgccagtggc gaagccgaag tcaaagccgt cgagcgtctt
     2701 gatcgtcgga aagccggcga cccgagcaaa catctcgcgg gctcgggcgc tgcgcgcagc
     2761 acgctcggcc cgcagcgtct cttcaagaaa gtcagcgaag gaggccgctt tcgccgccgc
     2821 gctttgggcg atgccggaat acaagttggg catcgcgctc aaccgcagct cctggcacag
     2881 ctcgacgata cgctgatgct gcaactcgct catgacgcgc caccggcaaa cgcgtcataa
     2941 aacgacagcg ggtgctgcag gccgatgatc ggtactggca ccggcttctg gagcgagcgc
     3001 acgctgcggc cggcgtaagg cgtcggtacc ggctgcaact gcactctctc gatcgccagc
     3061 cgttcggccg ggatctcgcc ggtggtgccg tgcacgcgca cgttcgccac ctcgcgcagc
     3121 catcggccga ccgccaggtt cgcggtctcg cggtcc
//
LOCUS       DATLJT010001583         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2453419,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001583 DATLJT010000000
VERSION     DATLJT010001583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453419"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VM910_36285"
     CDS             complement(<1..241)
                     /locus_tag="VM910_36285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68000.1"
                     /translation="MFRSKRANRVKISAWDGPLNPWFKGDVVSMGAVADSKLKPAVNF
                     RIRGFQARACKRFGGTQIGRKGANGLNISARNLAEY"
     gene            complement(523..1592)
                     /locus_tag="VM910_36290"
     CDS             complement(join(523..1085,1085..1592))
                     /locus_tag="VM910_36290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553005.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI68001.1"
                     /translation="MSHAIPVRADYTAGEVRQLAQRAKDAAQARRLLAIAAVLDGASR
                     EEAAKIGSMDRQTLRDWVIRFNEQGADGLINIPSPGARPKLDARHTAFLARIVEEGPI
                     PAIHGVVRWRACDLIMRLYEEFGLSVSDDTVYRALKKLGFSHVSARPKAYKQNAEAME
                     AFKKTSPSVLAEIRAALAPGTPVEVWFQDEMRVGQKNKLTYRWARKGSRPRAAHDQRT
                     QSTYLFGAVCPERGAGAALLLPACNCEAMQLHLDEIATKVIPGAHAIVLLDQAGWHGA
                     KALVVPSNVSLVPLPPRAPELNGQENIWQFMRQNWLSNRIFKSFDDIVDHCCYAWNTL
                     IDQPWKIMSIARRDWAAVGHSI"
     gene            complement(1951..>2386)
                     /locus_tag="VM910_36295"
     CDS             complement(1951..>2386)
                     /locus_tag="VM910_36295"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68002.1"
                     /translation="SYGRNTAAPLMFHIFDLLPPEPILPIAAQPNAGGLARRVGAALK
                     RFIPSADVATTVANRTSPPRITFPPNGAHVVLARNGSSFAPLALEAMGGAPPYRWAVN
                     GQPLAPAAIGAPTAWMPDGPGFVRISVTDHHHKSITADVWIE"
BASE COUNT          486 a          717 c          713 g          470 t
ORIGIN      
        1 cgtactcggc gagattgcga gcggaaatat tcagaccgtt tgcgcctttc cgaccgattt
       61 gagtgcctcc aaatcgtttg caggcacggg cttggaagcc gcggattcga aaattgacgg
      121 caggcttgag ctttgaatcg gctaccgccc ccatcgacac gacatcgccc ttgaaccagg
      181 ggttcagcgg accgtcccaa gctgagattt ttacacggtt ggcccgtttg gagcggaaca
      241 cgcaatcgtt tgcgcatgct tgttaagcgg cgctggcccc gatcacaatc tcgatcaacc
      301 atccatctgg gcgcctcgac gcagtcagca cgtcggcggt tgggccagtt ccacgatcac
      361 agcgctgcgc atccgggcgc catctaacac ctctgcggcg ttaacggatg ccggtgtgta
      421 acggtgcaca ccgactcgca gctcgcgtga acggtttgaa ttttcagaca ggaaaagctt
      481 cgccacggag cggcgcagtc ttacaacgcc tcataccaat cctcagattg agtgaccgac
      541 tgccgcccaa tcgcgtcgcg cgatggacat gatcttccag ggttggtcaa tgagtgtgtt
      601 ccaagcgtag cagcagtggt cgacgatatc gtcgaaggat ttgaaaatcc gattagacag
      661 ccaattctga cgcatgaact gccagatgtt ttcctgaccg ttgagttcgg gtgcgcgcgg
      721 cggcagcggc acgagcgaga cgttgctcgg aaccaccagg gctttggcgc catgccagcc
      781 ggcctgatca agcagcacaa tggcgtgcgc cccggggatg actttggtcg cgatctcgtc
      841 gagatgtagc tgcatggctt cgcagttgca ggccggcagc aagagggcgg cgccggctcc
      901 gcgttcgggg cagaccgcac cgaacagata ggtcgattgc gtgcgttgat catgggcggc
      961 acgggggcgt gagcctttcc tggcccaacg gtaggtcagc ttgttctttt gacccacccg
     1021 catctcgtct tggaaccaga cctctaccgg tgtgccgggt gcgagtgccg cgcggatttc
     1081 cgccacacgc tcggcgaagt ttttttgaat gcctccatag cctcggcatt ctgcttgtag
     1141 gccttcggcc gggcgctcac atgcgagaag cccaacttct tgagggcgcg atagaccgtg
     1201 tcgtccgaga ccgatagccc gaactcctca tacagccgca tgatcagatc gcacgcccgc
     1261 cagcgcacca cgccatggat cgcagggatc gggccctcct ccacgatgcg ggcgagaaag
     1321 gccgtatgtc gggcgtcgag cttgggccgc gctcccggag aaggaatgtt gatgaggccg
     1381 tccgctccct gctcgttgaa ccggatcacc cagtcccgca gggtctgacg atccatacta
     1441 ccaatcttgg ccgcctcctc ccgcgaggcg ccgtcgagca cggccgcaat cgctaataac
     1501 cgtcgcgcct gcgcagcatc tttcgctcgc tgggcaagct ggcgaacctc gccggccgta
     1561 tagtccgccc gcactggaat agcgtggctc atggcaaacc ccctcagttt gccatcttga
     1621 atcatgccgc gatcgattcg gaaatcccca aagagtcagc cgctcagcgg cttggtatca
     1681 gtccgacgac cgatcaaggc ctcaggcggc aaacaccgct ctccgacatc agaatattgc
     1741 ttaggggttt gaagcaagtg gcgcgtactg actcaacgtc gaggccctaa ttttcctaga
     1801 taaccgtgga ccggcgattt tacacgcacc tgctcacatc ttagcgctaa taatttcaat
     1861 gggacgcatc tgattctaaa agcagatgca gcaggcggtg actggcaccc atcaattgat
     1921 ctggacctga actcgggctt gaccatcttt ctactcaatc cacacatcgg cggtgatgga
     1981 cttgtgatgg tgatccgtca ccgagatcct gacaaaaccc ggaccatccg gcatccaagc
     2041 agttggcgcc ccaattgccg caggcgccaa gggctggccg ttgacggccc aacggtaagg
     2101 cggcgcaccg cccatggcct cgagagcgag tggcgcgaaa gatgagccat tccgggcaag
     2161 cacgacatgt gcgccgtttg gaggaaaggt tatgcgcgga ggtgacgtgc gattggcaac
     2221 ggtggttgca acgtcagccg acgggatgaa acgtttcagg gcggcgccaa cgcggcgtgc
     2281 gaggccgccc gcgtttggct gagccgcaat cggaaggatt ggctccggcg gcaggagatc
     2341 aaagatgtgg aacatgagcg gagctgccgt gttgcgacca tacgac
//
LOCUS       DATLJT010001584         2216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3371932,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001584 DATLJT010000000
VERSION     DATLJT010001584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3371932"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1258
                     /gene="rho"
                     /locus_tag="VM910_36300"
     CDS             2..1258
                     /gene="rho"
                     /locus_tag="VM910_36300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVI68003.1"
                     /translation="MKLQELKAKTPAELVSFAEELEVENASTMRKQELMFGILKQLSA
                     RETDIVGEGVVEVLPDGFGFLRSPEANYLPGPDDIYVSPSQIRRFGLRTGDTIDGHIR
                     SPKEGERYFALLKVNSLNFEDPEKARHKVNFDNLTPLYPDEWLKMEYEDPTKKDLSAR
                     VIDIVAPVGKGQRALIVSPPRTGKTVLLQNIAHSITHNHPEVYLIVLLIDERPEEVTD
                     MQRSVKGEVISSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSELILDRKVADKRTFPAMDITRSGTRKEELLVPPDQLKKMYVLRRILNPMGT
                     MDAIDFLLDKLRNTKNNGEFFDSMNT"
     gene            complement(1347..>2216)
                     /locus_tag="VM910_36305"
     CDS             complement(1347..>2216)
                     /locus_tag="VM910_36305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVI68004.1"
                     /translation="REKLLSVRALRVRPGLDDKVLADWNGLMIAALANAGSMLDEPRW
                     LELASRAFDFVAQSMARGDRLGHSWRQGKLKFPGLASDYAAMVRAALALHEATGAASF
                     LDQALQWQQALDRDYINAENGTYYLTAANAEGLVIRPAATTDEATPNHNAVAAQNLIR
                     LAVLSGDDAWRDKADRLIAAVAPLIAENLYMHVAMLNAIDLRLRGTEIVVTGRGAGAD
                     ALLAAARRLPPLDRIVLHAPSAEAPRDKHSARDKLNASREPQAFVCVGETCSLPIRDA
                     AALGSAIEAMRRS"
BASE COUNT          455 a          699 c          668 g          394 t
ORIGIN      
        1 aatgaaactg caagaactca aggccaagac tccggccgag cttgtctcct ttgccgaaga
       61 attggaggtc gagaacgcct cgaccatgcg caagcaggag ctgatgttcg gcatcctcaa
      121 gcagctctcg gcgcgcgaga ctgacattgt cggcgagggc gtcgtcgagg tgctgccgga
      181 cggcttcggc tttttgcgat cgccggaagc gaactacctc cccggacccg acgacattta
      241 cgtctcgccg tcgcaaattc ggcgcttcgg actgcgcacc ggcgacacca tcgatggtca
      301 catccgcagt cccaaagagg gcgaacgcta tttcgcgctg ctcaaagtca attcgctcaa
      361 tttcgaagat ccggaaaaag cgcggcacaa agtcaatttc gacaatttaa cgccgctcta
      421 tccggacgag tggctgaaaa tggaatacga ggatccgacc aaaaaggatc tctcggcgcg
      481 cgtcatcgat attgtcgcgc cggtcggcaa gggacagcgc gcgttgatcg tgtcgccgcc
      541 gcgtaccggc aagacggtgc tgctgcaaaa catcgcgcac tcgatcactc acaaccatcc
      601 ggaagtctat ctaatcgtgc tgttgatcga cgagcgtccg gaggaagtca ccgatatgca
      661 gcgctcggtg aaaggcgagg tgatctcgtc gaccttcgat gagccagcgg tgcgccacgt
      721 ccaggtcgcc gagatggtga tcgaaaaagc aaagcggctc gtcgagcacg gccgcgatgt
      781 ggtgatcctc ctcgattcga tcacgcggct cggccgcgcc tacaacaccg tcgtgccatc
      841 gtcgggcaag gtgctcaccg gcggcgtcga cgccaacgcg ttgcagcggc cgaagcgctt
      901 cttcggcgcc gcgcgcaaca ttgaagaagg tggctcgctc acgatcatcg ccaccgcgct
      961 gatcgatacc ggcagccgca tggacgaagt gatcttcgaa gaattcaagg gcaccggcaa
     1021 ttcggagctc atcctcgacc gcaaagtcgc cgacaagcgc accttccccg ccatggacat
     1081 cacccggtct ggcacccgca aggaagagct gttggtgccg ccagatcagc tcaaaaagat
     1141 gtacgtgctg cgccgcatcc tcaatccgat gggaacgatg gacgcgatcg actttctgct
     1201 cgacaagctg cgcaatacca agaacaacgg cgagttcttc gactcgatga atacctaagc
     1261 gcggcttgcg ccgacgattc ctgttctcgc aacatggtcc cgaagggttc agctcgggtc
     1321 gcgacattgc gtcggagtcg acctttttag gagcgccgca tcgcctcaat cgcactgccg
     1381 agcgcggcag catctctaat cggcagcgaa caggtctcgc ccacacagac gaaagcttgc
     1441 ggctcgcgcg aggcatttaa cttgtcgcgc gccgagtgct tatctcgcgg cgcctcggca
     1501 gaaggggcgt gtagcacgat gcgatcaagc ggcggcaggc ggcgcgcggc agcaagcagc
     1561 gcgtcggcgc ccgcgccccg ccccgtcacc acgatttcgg tgccgcgcag ccgcagatcg
     1621 atggcgttga gcatcgccac gtgcatgtag aggttctcgg cgatgagcgg cgcaacggcc
     1681 gcgatcaatc ggtccgcttt gtcacgccag gcatcatcgc cggacagcac cgccagccgg
     1741 atgagatttt gcgccgcgac cgcgttgtgg tttggcgtcg cctcatccgt cgtcgcggcc
     1801 gggcgaataa cgagcccttc cgcgttggcg gcggtcagat aataggtgcc gttctccgca
     1861 ttgatgtaat cgcgatccaa cgcttgctgc cattgcagcg cctgatcgag gaagctcgcc
     1921 gcgcccgtcg cttcatggag cgcgagcgca gcgcggacca tcgcggcata atccgaagcg
     1981 agccccggaa atttgagctt gccttgacgc caagaatggc cgaggcgatc gccgcgcgcc
     2041 atgctttggg caacgaagtc aaaagcgcgg ctggcgagtt ccaaccatcg cggctcatcc
     2101 agcatcgatc cggcattggc gagtgcagcg atcattagcc cgttccaatc ggcgagcacc
     2161 ttatcgtcga ggccgggccg cacccgtagc gcgcgcacgg acaggagctt ttcgcg
//
LOCUS       DATLJT010001585         5371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_617127,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001585 DATLJT010000000
VERSION     DATLJT010001585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617127"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VM910_36310"
     CDS             complement(<1..854)
                     /locus_tag="VM910_36310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HVI68005.1"
                     /translation="MPRALMIQGAGSDVGKSLIVAGLARAATRRGLRVLPFKPQNMSN
                     NAAVTVDGGEIGRAQALQAQAAGVEPHTDMNPVLLKPETDIGAQIIVHGKRVATLHAR
                     DYAELKPSLLPAVLDSFERLKDRCDLVLVEGAGSPAEVNLRNNDIANMGFARSADVPV
                     VLVGDIERGGVIAQMVGIESVLEPEDAAMIVGFIINKFRGDPSLFEAGYKLIEARTGW
                     RGLGVLPYFPRARELPAEDALGLSEARKTGRQKVAFLALARIANFDDLDPLKLEPDVD
                     LVMVRPGEA"
     gene            complement(865..1485)
                     /gene="cobO"
                     /locus_tag="VM910_36315"
     CDS             complement(865..1485)
                     /gene="cobO"
                     /locus_tag="VM910_36315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVI68006.1"
                     /translation="MVSEQDPEEARHAAKMARKKAARDKIMSGKSGEKGLIIVHTGAG
                     KGKSSSAFGMVLRCVAHGFPCAVVQFIKGAWDTGERRLLTSHFGELCQFHAMGEGFTW
                     ETQDRARDIAAARAGWEKAKALIGDARLRMVVLDEINIALRYDYLDVNEVVDFLKTSK
                     PEMTHVVLTGRNAKQELIDVADLVTEMTLVKHPFRSGIKAQPGVEF"
     regulatory      1688..1906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1996..2370
                     /locus_tag="VM910_36320"
     CDS             1996..2370
                     /locus_tag="VM910_36320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HVI68007.1"
                     /translation="MATTLHVCITCRAGQTVAEGEPTPGKLLHQAIVAIGAPPGVTIV
                     PVECLSACNNGCSVALSAPGRWSYVYGRMSQANARDIVAGAAAYAAAPDGIVPWRSRP
                     EIFRKQSLARIPPIPALPEAAE"
     gene            2367..3401
                     /gene="cobW"
                     /locus_tag="VM910_36325"
     CDS             2367..3401
                     /gene="cobW"
                     /locus_tag="VM910_36325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HVI68008.1"
                     /translation="MTSLAKIPVTVITGFLGSGKTTLIRHLMTNADGKKLAVLVNEFG
                     SEGVDGEILKSCADANCPEENIVELANGCICCTVADDFIPTMQTLLARKVRPDHILIE
                     TSGLALPKPLLKAFDWPEIRSRITVDGVIALADAEAVAAGRFAPDPSAVEAQRAADES
                     IDHETPLSEVFEDQVSCADIVLLTKADLAGEAGLAAARRAIAAEAPRPVPMLAVTDGA
                     IDPQVILGLNAAAEDDLAARPSHHDGEEEHEHDDFASVVVELPEVTDVEALVAAIGRL
                     SREQNVLRAKGFLAVKGKPMRLLVQSVGERVRHQFDKPWGANPRQSKLVVIGEHGDID
                     EEAIRAGLGL"
     gene            3403..>5371
                     /locus_tag="VM910_36330"
     CDS             3403..>5371
                     /locus_tag="VM910_36330"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HVI68009.1"
                     /translation="MHVIFRESHGLEETATPVDLGQDGADLVVLSFSDSDLSAFAAGW
                     RRGRDRLPSLRLANLIALRHPLSVDTYLERTLSQARGILVRLIGGESYWAYGIAAVHQ
                     LAKARRIALAVLPADGRDDARLDALSTLPRSTLRRLRVLCDAGGAVAAQAALAQLALA
                     SGLYAGPVFGETQIADMGFYDPEKGPLATLPAADGRPHALVAFYRSYLTAADTGPVDA
                     LILLLRQKGFDAYGAFATSLKAPGVADWLKGHLTARPAAAIVNATAFSSIGDDGTTPF
                     DAAACPVFQVALSTARREDWASSLRGLSPTDLAMHVVLPEVDGRILAGVVSFKSAGER
                     DGDLQFAHLAHRADDERVAAAVERVSAWHRLATTRSRDKQLAVVLSSYPGRPHQIAHA
                     VGLDALASVEALLGDLARAGFDVEPVDALGQTLREETLTWSLADYCAALEKLPAALRK
                     NLQSSWGAPEDDPQCRDAAFHFAGLRRGKAIIAVQPERGEVDRRDADYHDLSRTPRHA
                     YVAFYLWLRQQGIDALIHMGAHGTLEWLPGKSVALSDSCWPEALTGDLPVIYPFIVND
                     PGEAAQAKRRIGAVTIGHLPPPLTQGALPQNLVRLEQLLDEYSSADGLDPVRRQRLVA
                     AIRDEARTSGVEDDLGLSATHTPAEAISRIDR"
BASE COUNT          897 a         1765 c         1723 g          986 t
ORIGIN      
        1 gcttcgcccg gccgcaccat gacgagatcg acgtcgggct cgagcttgag cggatcgaga
       61 tcgtcgaaat tggcaatgcg cgcgagcgca agaaacgcga ctttctgtct gcctgttttg
      121 cgcgcctcgg aaagcccgag cgcatcttcc gcaggcagtt cccgcgcgcg aggaaagtag
      181 ggcagaacgc cgagaccgcg ccagcccgtg cgcgcttcga tcagcttgta gccggcctcg
      241 aacagcgacg gatcgccgcg gaacttgttg atgatgaagc caacgatcat cgcagcgtct
      301 tcaggctcga ggaccgactc gatcccgacc atttgcgcga tgacgccgcc gcgctcgata
      361 tcgccgacga gaaccacggg cacgtcggcg ctccgcgcaa atcccatatt ggctatgtcg
      421 ttgttgcgca ggttcacttc ggcggggctg ccggcgccct cgaccagaac gagatcgcac
      481 cggtccttca gccgctcgaa gctgtcgagc acggccggca agagcgaagg tttgagctcc
      541 gcatagtcgc gcgcgtgcaa cgtcgcgacg cgcttgccgt ggacgatgat ttgcgcgccg
      601 atgtcggttt ccggcttcag cagcaccggg ttcatgtcgg tgtgcggctc gacgcccgct
      661 gcctgcgcct gcagcgcctg ggcgcggccg atctcgccgc catcgacggt gacggccgca
      721 ttgttggaca tgttctgcgg cttgaagggc agcacccgaa ggccacgccg cgttgcggcg
      781 cgcgccagcc ccgcgacgat cagcgatttg cccacgtccg aacccgctcc ctggatcatg
      841 agagcgcgcg gcatggcggg gagcctagaa ctcgacaccg ggctgagcct tgatgccgga
      901 ccggaagggg tgcttcacca gcgtcatctc ggtgacgaga tcggcaacgt cgatcaactc
      961 ctgcttggca ttgcggccgg tgagcacgac atgtgtcatc tcaggcttcg atgtcttcag
     1021 gaaatcgacg acctcgttca cgtcgaggta atcgtagcgc aacgcgatat tgatctcgtc
     1081 gagcacgacc atgcgcaagc gtgcgtcgcc gatcagcgct ttcgctttct cccagcctgc
     1141 ccgcgcggct gcgatgtcgc gggcgcggtc ctgcgtttcc caggtaaagc cttcgcccat
     1201 cgcatggaac tgacagagct cgccgaaatg tgaggtcagc agccttcgct cgccggtatc
     1261 ccaggcgccc ttgatgaact gcacgaccgc acagggaaag ccgtgcgcga cgcagcgcag
     1321 gaccatgccg aaggcggagg aggattttcc ctttcccgcg ccggtatgga cgatgatcaa
     1381 gcccttctcg ccgctctttc cggacatgat cttgtcgcgc gctgctttct tcctggccat
     1441 tttggcggcg tggcgggcct cctccggatc ctgttcggaa accataccag tgtccccggt
     1501 tctgacattc gtgttgctgg gaaacccttt tacgaatgtc agaaccaaag gaacactgga
     1561 aatctctggg attctcgtgc cgcccgggat ttgacatgcg ctttgaagga cttgcggcga
     1621 taccgcagcg aatgtcaaat ccgccgcatg aaattctcct gcggcttgac aaggtccacg
     1681 cttgggcaaa tggtgggccc gcgttggttc ctgtcccatg acaggcgaag agggaatgcg
     1741 atagggctcg aagtctccaa ggcttgagcc ccgaaaagca gccgcccccg cgaccgtgac
     1801 cggagagatc ccgaggcgcc actgaccgaa aggtcgggaa ggcggggatc ggaggagaaa
     1861 tcctgctccg caagccggga gacctgccga cgcaagtttt tcgaccggcg gacggggtgt
     1921 tccgcgacgg tgagcgaacc tccccgatgg ttcgtccacc tcatcgctcc caccgcaagt
     1981 ttcagggaag gggcgatggc caccacactt catgtctgca tcacgtgccg cgccggccag
     2041 accgttgcgg aaggcgagcc gacgcccggc aagctgctgc atcaggccat tgtcgccatc
     2101 ggcgcgccac ccggcgtgac gatcgtgccg gtggaatgcc tgtccgcctg caacaacggt
     2161 tgctcggtcg cgctcagcgc gccgggccgg tggtcctatg tctatggccg aatgtcgcaa
     2221 gccaatgcgc gggatatcgt cgctggcgcc gcggcctatg cggccgcgcc cgacggcatc
     2281 gtgccgtggc gctcgcggcc cgagattttc cgcaaacaat cgcttgcccg cattcccccg
     2341 attcccgcct tgccggaggc cgccgaatga cctcgctcgc caaaattccc gtcaccgtca
     2401 tcaccggctt tctcggatcg ggcaaaacca cgctgatccg gcatttgatg accaatgcag
     2461 acggcaagaa gctcgccgtg ctggtcaacg aattcggcag cgagggtgtt gatggcgaga
     2521 tcttgaaatc ctgcgcggat gccaactgcc ccgaggaaaa catcgtcgag ctcgccaatg
     2581 gctgcatctg ctgcacggtc gcggacgatt tcattccgac catgcagacc ctgctggcgc
     2641 gaaaagtccg ccccgatcac atcctgatcg aaacgtccgg gcttgcgttg ccaaagccgc
     2701 tgctcaaggc cttcgactgg cctgagatcc gctcgcgcat cacggtcgat ggcgtgatcg
     2761 cgctggccga cgcggaggcc gttgccgcgg ggcgttttgc gccggatcct tcggcagtcg
     2821 aggcgcagcg cgccgccgat gaaagcatcg accacgagac gccgctgtcg gaagtgttcg
     2881 aggatcaggt ctcctgcgcc gacatcgtgc tgctcaccaa ggccgatctt gccggcgaag
     2941 ccggtcttgc tgcggcaagg cgcgcgatcg cggcggaggc gccgcgtcca gtgcctatgc
     3001 tcgccgtcac cgacggcgcg atcgatccgc aggtgatcct cggcctcaac gccgcggcgg
     3061 aggacgatct tgccgcgcgt ccctcgcacc acgacggcga ggaggagcac gagcatgacg
     3121 atttcgcctc agtcgtggtc gagcttcccg aggtgaccga cgtcgaagcg ctggttgccg
     3181 ccatcgggcg gctctcgcgc gagcagaacg tgctgcgtgc caagggattc ctggcggtca
     3241 agggcaagcc gatgcggcta ttggtgcagt cggtcggcga acgtgtgcgc caccagttcg
     3301 acaagccctg gggtgcgaac ccgcggcaat caaagctcgt cgtgatcggc gagcacggcg
     3361 atatcgacga agaggcgata agggcgggat tgggtctttg agatgcatgt catcttccgc
     3421 gagagccacg gcctggaaga aaccgcgacg cctgtcgatc tcggtcagga tggcgccgat
     3481 ctcgtggtgc tttcgttctc cgacagcgat ctttcggcct ttgccgcagg ctggcggcgc
     3541 ggtcgcgatc gcttgccctc gctcaggctc gccaatctga tcgcgcttcg ccatccgctt
     3601 tcggtcgaca cctatctcga gcgcacgctg tcgcaagcgc ggggaatcct ggtacggctg
     3661 atcgggggag agtcctactg ggcttacggc atcgccgccg tgcatcagct cgcgaaggct
     3721 cgccgcattg cgctcgcggt cctgcccgcc gacgggcgcg acgatgcgcg gctcgatgct
     3781 ctctcgaccc tgccacgctc gaccttgagg cggctgcgcg tgctgtgcga tgccggcggc
     3841 gcggtggcgg cacaggcggc actggcccag ctcgcgcttg catccggact ttacgcaggc
     3901 ccggtgttcg gcgagacgca aattgcggac atgggctttt atgatccgga gaagggaccg
     3961 cttgcgaccc tgccggccgc cgatggccgg ccgcacgcgc ttgtcgcctt ctaccgctcc
     4021 tatctcacgg ccgccgacac cgggccggtc gatgcgctga tccttctgtt gcggcaaaag
     4081 ggctttgacg cctatggcgc gttcgccacc tcgctcaaag cccccggcgt cgccgattgg
     4141 ctgaaaggac atctcaccgc gcgcccggca gcggcgatcg tgaacgcgac cgcgttttct
     4201 tccatcggcg acgacggcac gacgccgttt gacgctgcgg cctgcccggt gttccaggtc
     4261 gcgctctcga ccgcgcgacg cgaggactgg gcctcctcgc tgcgcggcct gtcgccaacc
     4321 gatcttgcca tgcatgtggt gctgccggag gtggacgggc ggattcttgc cggcgtcgtc
     4381 agcttcaaat cggcaggcga gcgcgacggc gacttgcagt tcgcgcatct tgcgcatcgg
     4441 gcggacgacg aacgcgtcgc agcagcggtc gaacgcgtgt cggcgtggca tcgcctcgcg
     4501 acgacgcgtt cccgtgacaa gcagcttgcc gttgttctct cgagctatcc cggccggccc
     4561 caccagatcg cgcatgcggt cggactcgat gcgctcgcct ccgtcgaagc cttgcttggc
     4621 gatctcgcaa gggccggctt cgatgtcgag cccgttgatg cgctggggca aacgcttcgc
     4681 gaggagacat tgacctggag cctcgccgac tattgcgcgg cgctcgagaa acttcccgcc
     4741 gcacttcgaa agaatctgca aagctcatgg ggcgcgccgg aagacgatcc gcaatgccgc
     4801 gatgctgcct ttcatttcgc cgggctccgt cgcggcaagg caatcattgc cgtgcagccc
     4861 gaacgcggcg aggtggacag gcgggatgcc gattatcacg atctgtcgcg gacgccgcgc
     4921 catgcctatg tcgcgtttta tctctggctc cggcaacagg gcatcgacgc gctgatccac
     4981 atgggtgcgc atggcacgct ggaatggctg cccggcaagt cggtggcgtt gtccgattcc
     5041 tgctggccgg aggcgctgac cggcgatctg cccgtcatct atcccttcat cgtcaacgat
     5101 cccggcgagg cggcgcaggc caagcgccgc attggtgccg tcactatcgg ccatcttccg
     5161 ccgccgctga cacaaggcgc gctgcctcaa aatctcgtcc ggctcgagca gttgctcgac
     5221 gaatattcga gcgccgatgg tctcgatccc gtgcggcggc agcgtctcgt cgcggcgatc
     5281 cgcgacgagg cgcgcacgtc aggcgtggaa gatgatcttg gactgtcggc cactcacacg
     5341 cctgccgaag cgatttcgcg gatcgaccgt t
//
LOCUS       DATLJT010001586         9885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_154689,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001586 DATLJT010000000
VERSION     DATLJT010001586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154689"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1209
                     /locus_tag="VM910_36335"
     CDS             370..1209
                     /locus_tag="VM910_36335"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HVI68010.1"
                     /translation="MIRYVLSTTGAALLLATFAYAQESPPLRAAVDGTFAPHAFPNLS
                     GGYQGFNIDLANEIAKRLKRKIMIDAAQFSGLVPALQAGTYDFLAAPTTVTKERAESM
                     LFTEGYLNTDFQFLIRKGSPKVVQLEDLKGKTISVNKGSAYDSWARELEGKIGWKVES
                     FGTQTDAVQAVLVGRADANVAGNTVIAWAVKNNPQLELSYLYSTGLVWAAPLRKDSSE
                     LRKALDVGVECMKLDGTIAKMHEKWFGVAPAAGSAAVTVFPGYGVPDMPGYDPTAHTP
                     SCS"
     gene            1261..2007
                     /locus_tag="VM910_36340"
     CDS             1261..2007
                     /locus_tag="VM910_36340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVI68011.1"
                     /translation="MTTGAPPILEIRGLRKRFHDVEVLRGIDLVVLPQQLVFVIGPSG
                     SGKSTLLRCCNRLEEASAGSIRIDGVDITARSTDINAMRRQIGMVFQSFNLYPHMTAL
                     RNVTLALAKVLGKPRAEAERIGTAALARVGLADKARSYPGELSGGQQQRVAIARALAL
                     EPKVMLFDEPTSALDPELVGSVLNVMRELKRGGMTMLVVSHEMRFARDAADRVIFMDH
                     GLIVEEGPPQQMFSAPSEARTRAFIAELAR"
     gene            2015..2698
                     /locus_tag="VM910_36345"
     CDS             2015..2698
                     /locus_tag="VM910_36345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404523.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI68012.1"
                     /translation="MEAWSRFLDAFFNWDVIQRYFPAVMKGVVVTIEIAAAVVITGTL
                     LGLALAIVRTFRLLAVNALIVVFVDMFRALPPLVLVLLVYFGLPNIGINLPSFAVLWL
                     VLALVLAAFAEEIFWAGILSIPKGQWEAARSTGLGFLGTLAYIVLPQAIRLTVPPLTN
                     RTIAITKNTALGTVIGVGEILNEATTAQSFSGNATPLMMGAIAYVILFIPVVVLGRFL
                     ETRFVWHRV"
     gene            2698..3363
                     /locus_tag="VM910_36350"
     CDS             2698..3363
                     /locus_tag="VM910_36350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI68013.1"
                     /translation="METIVQQFFNLAIMERALPLVLAGLEQTIVICLIVIPLGLAGGL
                     AFAIVSLSRLRSVRWAAVAWIDFFRAIPPLVLLIFIYSGLPFAGLRPSPMTAVAIAFF
                     LNTSSYYGEIYRAGIESVGHGQWDAARSTGLRGYQTLAYVVLPQAVRNVLPDLVSNTV
                     EVVKLTSIASVVALPELLYSADMARSVTFNSSPIVLAAAIYLAMLWPIVRLISRLERR
                     VAA"
     gene            3413..4315
                     /locus_tag="VM910_36355"
     CDS             3413..4315
                     /locus_tag="VM910_36355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HVI68014.1"
                     /translation="MGPIHKADSRQAVVRRMLDLLDRAKAQKCDLVVYPELALTTFFP
                     RWYMTEQAEVDAWFEREMPSAATRPLFEQAADDRIGLSFGYAELTADGHHFNTSILVD
                     RDGKIVGKYRKVHLPGHSEFDPQRAFQHLEKRYFEPGDLGFPVWRTMGGIVGMCICND
                     RRWPETYRVMGLQGVELIVLGYNTPSVNSQKADEGPEKRMFHHRLTLQAGAYQNSTWV
                     VAVAKGGAEDGFPLLAGSIIVNPDGEIVAEAKTEDDELIVADCDLDATIFGKETIFDF
                     QRHRRIEHYGRITNQTGVMLPPQS"
     gene            complement(4429..5097)
                     /locus_tag="VM910_36360"
     CDS             complement(4429..5097)
                     /locus_tag="VM910_36360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVI68015.1"
                     /translation="MSDPLPRIRVVNPNSNEDVTRGLDAALEPLRFANGPEIVCSTLA
                     EGPFGIETQADVESVVMPLRRMVEADNSSDAFVIACYSDPGLHVCREGTTRPVFGIAE
                     CGVLTALARAGRFGVIAISQRSIARHRRYLLQMGLTDRLAGERPLEISVAETATGEQT
                     LRRMVDVGRQLKEQDGAEAVIMGCAGMARHRRPLEAALGIPVIDPTQAAVTMAFGALQ
                     FSRP"
     gene            5408..6646
                     /locus_tag="VM910_36365"
     CDS             5408..6646
                     /locus_tag="VM910_36365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI68016.1"
                     /translation="MKVCMFHLMPYRDLPADFERRYNSAYIDPVWFEVADSDKVGQFY
                     NATLDEMLYAAKAGLHGLCTNQHHQNVYGFMANPSIMGAVLARQTNGQNIAIIQLGST
                     LPSTTPPTRIAEEYAMLDCISGGRLVAGFPTGLPADATISNGVIPVEQRERFREALAL
                     VIKAWSARDIFAWNGRHYQLGMVNLWPRPIQQPHPPIWVPGSGISSTAEYVVGQDHCF
                     CHLSYYGAKNAEQVSDRYWELVARKGRDDNPYRFSFLQLIGVGETDAEAEDLYAEHAE
                     YFFHKLLYTPSYYQAIPGCLEYEALVQSLRHNPRASINLRELKAKDLFGRGFVVVGSP
                     KTVREQLIDGIKRLRMGHLLALLHFGSMPTTLCKKNIDLFAREVLPHLADLWDDKYED
                     RWWPERLKAKRPAAALAAAS"
     gene            6667..7467
                     /locus_tag="VM910_36370"
     CDS             6667..7467
                     /locus_tag="VM910_36370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68017.1"
                     /translation="MAAPEVRRLSLWQDRIQTEVEISGRGQPLVYLHGPWGLAPDRAF
                     VAGLAGGHTVYAPKFPGTTHGNPDAVHALDNWFDLILYYGELFDTLELSVPAVVGHSF
                     GGLVAAEFAASAPNSVGRLVLIDPVGLWRDDAPVKNWMILSDKVRRPSLFADSDGEAA
                     RRFFDVPSDPAERIDTLAQFIWAQACSGKFVWPIADRGFSKHAHRIAAPTLIVWGAAD
                     GIIPPVYAQEFARRIADAQVELIDHAGHLPHLEHPEAVTKVVRAFLGS"
     gene            7577..8857
                     /locus_tag="VM910_36375"
     CDS             7577..8857
                     /locus_tag="VM910_36375"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI68018.1"
                     /translation="MSTASTASVAVTPVTVSDRWTRYNATVLTICILGWAFDIYEATI
                     MQLVTPILIKEWGITPATMGTVTTISRWIGLIGTFAFPVLADLYGRKPALIWAILGYS
                     IFTGLTGFSTGWITLLIFSSITRIALAGENPVGMLMVTETAPTKWRATALGGLVGGYP
                     FGYMLCSLAALVVVPLWGWRSLYFLGILPALLVLWIRIGIKESPRYERVTAQMLKEGL
                     KKQLDILSPAREYPRETLIASLVYFFYLFTWLGWSAWMPFYLATEKKLGFQVMGSYLS
                     IWMFCAIFAYWICGWLCDVFGRRWVIPAFAVPAGALLIAMGHVDSPSSLFWVGLATNF
                     LITGSFGTGLGYTAELFPTQIRGTGVGASFTFGLGVASLAPMIMGWIATAHSVAAGLP
                     LLACSFLLLAPLFIWFAPDTTRKELTDFVGQKAG"
     gene            8921..9712
                     /locus_tag="VM910_36380"
     CDS             8921..9712
                     /locus_tag="VM910_36380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68019.1"
                     /translation="MTADPASSIVVNNVRVELIERGHGKPLLFLHPEIGIEPAAPVLD
                     QLASRMRVLAPVHPGFGRSEQPKAFDTVDDLAYFYLDLLEHLDLRQVTVVGVSFGGWI
                     AAEIAIKSTERLSHLVLANAVGIKVGDRETRDIVDIYAIPQSEYVQLAYFNPDVGTHD
                     YKTMPDARVLAAARNREATARYAWSPYMHDPKLLGRVHRIDIPTLVLWGANDRIVFES
                     YGRAYCAAITGARFELIERASHFPHLEQPEIFADRIFSFVGETSQ"
BASE COUNT         1720 a         3146 c         3027 g         1992 t
ORIGIN      
        1 ttcccagaac ggtcagcccc tgcacgccac attgcagata gaattcgacg aggttgtcga
       61 cactcggcca atcgatgtcg ccggtatcgg tgaacggcgt tgccgagatc gtgtacacgc
      121 ccttcgcgga cgcatcgagc aattggctca tggttctgcc ttccctcgga tccgctattt
      181 ttgagtttgc cgggaatgcc gtcgcaacaa atctatacct tgcatgtggc gcagggcaac
      241 cgatccgacg tggcctggat cggcatcgcg cacgcatctc ggctcattgc ctgcgcattc
      301 gtcttgtccg gtttgatccg gttgctatag tccaatgaat gctcgctggg cgggcttgag
      361 gggagctcca tgatccgata tgtgctttcg accaccggtg ccgcactgct tctcgccacg
      421 tttgcctatg ctcaggagtc gccgccgctt cgcgcggcgg tcgatggcac gttcgcaccg
      481 catgcctttc caaatctgtc cggcggttat cagggcttca atatcgatct cgccaacgag
      541 atcgccaagc ggctcaagcg caagatcatg atcgatgctg cgcagttctc cggcctcgtg
      601 ccggcgctgc aggctggaac ttacgacttt ctcgcggcgc cgacgaccgt gaccaaggag
      661 cgcgccgaga gcatgctgtt caccgagggc tatctcaaca ccgactttca gttcctgatc
      721 aggaaaggat cgcccaaagt cgtgcagctc gaagacctta agggcaaaac tatttccgtc
      781 aacaaggggt cggcgtacga ctcctgggcg cgcgagctcg agggcaagat cggctggaag
      841 gtcgaatcct ttgggacaca gaccgacgca gtgcaggcgg ttctggtcgg acgtgccgac
      901 gccaatgtcg ccggcaatac agtcatcgca tgggcggtca agaacaaccc gcagctggag
      961 ctgtcatatc tgtattcgac gggtctcgtc tgggctgcgc cgctgcgcaa ggactccagc
     1021 gagctgcgca aggcgctcga cgtcggcgtc gaatgcatga aactcgatgg cacgatcgcg
     1081 aaaatgcacg aaaagtggtt cggcgtggcg ccagcggcgg gttccgccgc ggtgaccgtt
     1141 tttcccggtt atggcgttcc cgacatgcca ggctatgatc caactgcgca cacgccctcg
     1201 tgcagctgaa gcgggcggcg tgatgccttg tcgcgggcgc cgtggacgca ccgattggcg
     1261 atgacgaccg gcgcgccgcc catcctggaa atccgcggcc tgcgcaagag attccacgac
     1321 gtggaggtgc tgcgcgggat agaccttgtc gtgctgccgc aacaactggt ctttgtcatc
     1381 ggaccgtccg gatcaggcaa aagcaccttg ctgcgctgct gcaaccgcct ggaggaggcg
     1441 tctgccggct cgatccgcat tgacggcgtc gatatcacgg cgcgttccac cgatatcaac
     1501 gccatgcggc gccagatcgg catggtgttc cagtcattca acctctatcc gcacatgacg
     1561 gcgctgcgaa acgtcacgct cgcactcgcc aaggtgctgg gcaagccacg tgccgaagcg
     1621 gagcgaatag ggacggcggc gctcgctcgc gtgggccttg ccgacaaggc gcgatcctat
     1681 cccggcgaac tttcgggcgg gcagcagcag cgggtggcga tcgctcgcgc gctcgcgctc
     1741 gaacccaagg tgatgctgtt cgacgagccc acctccgcgc tcgacccgga gctcgtgggc
     1801 agcgtactca acgttatgcg cgagctcaaa cgcggcggca tgactatgct ggtggtgagt
     1861 cacgagatgc gcttcgcgcg cgacgccgcc gaccgcgtga tcttcatgga tcacgggctc
     1921 atcgtggagg agggcccgcc gcaacagatg ttttcggcgc ccagcgaggc gcgcacccgc
     1981 gccttcatcg ccgagctcgc gcgctgagat cgccatggag gcttggtcgc gctttctcga
     2041 cgcgttcttc aactgggacg tgatccagcg ctatttcccc gccgtcatga agggcgttgt
     2101 ggtgacgatc gaaatcgcgg cggcggtggt gatcaccggc acgctgctcg ggcttgcgct
     2161 tgcgatcgtg cgaacgttcc gtctgcttgc ggtgaatgct ctgatcgtag tcttcgtcga
     2221 catgttccgg gcgctgccgc cgctcgttct cgttctttta gtctatttcg gcttgcctaa
     2281 catcggcatc aatctgccgt ccttcgcggt actttggctt gtgcttgcgc tggtgttggc
     2341 cgcctttgcc gaggagattt tttgggcggg catcctgtca atcccgaagg ggcagtggga
     2401 ggcggcgcgt tccaccggct tgggcttcct cggcacgctc gcctatatcg tgttgccgca
     2461 ggcaatccgc ctgaccgttc cgccactcac caaccgcacg atcgccatca ccaagaacac
     2521 ggcgctcggg accgtgatcg gggtgggtga aatcctcaat gaggctacca ccgcgcaatc
     2581 gttttccggc aatgctacgc cgctcatgat gggagcaatc gcctatgtaa tcctgttcat
     2641 cccggtcgtc gtgctcggcc ggttcctcga gacccggttt gtctggcaca gggtctgatg
     2701 gaaacgatcg tccagcagtt cttcaacctt gccatcatgg aacgagcgct cccgctcgtc
     2761 cttgccggac tcgagcagac gatcgttatt tgtctcatcg tcattccgct tggactggcc
     2821 gggggcctcg cattcgcgat cgtttcgctt tcgcgtttgc gcagcgtacg ctgggcggcg
     2881 gttgcatgga tcgatttctt tcgtgccatt ccgccgctcg tgctcctgat ctttatctat
     2941 tccgggttgc cgttcgccgg tctgcggccg tcgcccatga cggccgtcgc catcgccttc
     3001 ttcctcaaca cgtcgagcta ttatggcgag atctatcgtg ccggcatcga aagcgtcggc
     3061 cacggtcaat gggatgccgc gcgctcgacc ggcctacggg gctaccagac gctggcctat
     3121 gtcgtgctgc cgcaagcggt gcgcaacgtg ctgcccgatc tcgtgtccaa taccgtcgag
     3181 gtggtaaagc tcacgtcgat tgcgagcgtg gtggcgctgc ccgagctgct ctattcggcc
     3241 gacatggcgc ggtcggtaac cttcaattcg tcgccgatcg tgctggccgc agcgatctac
     3301 cttgctatgc tgtggccgat cgtgcgcctg atcagccggc tggagcggcg ggtcgccgcg
     3361 tgagccgcag gagagctccg tgagccgagt gcttcggatt ggcggcgccc agatgggccc
     3421 gatccacaag gcggacagcc ggcaggcggt cgtgcgccgc atgctcgatc tgctcgatcg
     3481 ggccaaagcc caaaagtgcg acctcgtggt ttatcccgag cttgcgctta ccaccttttt
     3541 cccgcgctgg tacatgaccg agcaagcgga agtagacgcc tggttcgaac gcgagatgcc
     3601 gagcgctgcg acgcgtccgc tgttcgagca ggccgctgac gatcgcatcg ggctgtcgtt
     3661 cggttatgcc gagctcaccg ccgatggcca tcatttcaac acctcgatcc tggtcgaccg
     3721 ggacggaaaa attgtcggca agtatcgcaa ggtgcatctg cccggccatt ccgagttcga
     3781 tccgcagcgt gccttccagc acctggagaa gcgttatttc gagccgggcg acctcggctt
     3841 tccggtctgg cgcaccatgg gcggcatcgt cggcatgtgt atctgcaacg accggcgctg
     3901 gcccgagacc tatcgggtca tggggttgca gggcgtcgaa ctgatcgtgc tcggctacaa
     3961 cacgccctcg gtcaattcgc agaaagccga cgaggggccg gaaaagcgca tgtttcacca
     4021 ccgactgacg ctccaggccg gcgcctatca gaactccacc tgggtcgtcg cggtggcaaa
     4081 gggcggcgcg gaggacggct ttcccctgct cgccggcagc atcatcgtca atcccgatgg
     4141 cgagatcgtc gccgaggcca agaccgagga tgatgaactg atcgttgccg actgcgacct
     4201 cgatgccacc attttcggca aggagacgat cttcgacttc cagcgccacc gccgcatcga
     4261 gcattacggc cgcatcacca accagaccgg cgtcatgctg ccaccgcagt cctgacatgg
     4321 ccgcgccgtt cgacgcggga tattcacggt gctgtcgcgc tggataggct tttgcctctg
     4381 atcgctggag agtggtgcgg cagactgagc ggcccgccgc aggcggcgtc aagggcggct
     4441 gaactgtagc gctccgaatg ccatggtcac cgcggcctgc gtcgggtcga tcaccgggat
     4501 gccgagtgcc gcttccagcg ggcgacggtg gcgcgccatt ccggcacagc ccatgatcac
     4561 cgcctccgcg ccgtcctgtt ccttcagctg gcgcccgacg tcgaccatgc gccggagcgt
     4621 ctgttcgccg gtcgccgtct cggcgaccga gatttcgagc ggacgttcgc cagcgaggcg
     4681 gtcggtgagc cccatctgaa gcagatagcg cctatgcctg gcgatcgagc gctggctgat
     4741 ggcaatgacg ccgaagcggc ccgcgcgcgc gagtgcggta agtacgccgc actcggcgat
     4801 gccgaatacc ggccgcgtgg tgccctcgcg gcagacgtgc aaccccggat cgctgtagca
     4861 tgcgatcacg aaggcgtcgg acgaattgtc cgcctcgacc atgcgtcgca acggcatcac
     4921 cacgctctcg acgtcggcct gggtctcgat gccgaacggc ccttccgcga gggtggaaca
     4981 cacaatctcc ggaccgtttg caaaccgcag cggctccaac gccgcgtcga gaccgcgagt
     5041 gacgtcctcg ttggaattcg gattgacgac gcgaatgcgc ggaagaggat cgctcatggc
     5101 tgctcgcgga cctgatctcg cctgcgacat caacacgatg ttggtcgtgt tgcaagcaat
     5161 gactgacgag cacgacgcga ttatgaccga cgtcgacgtc aaatctgcga aatcatcctc
     5221 ggacggtcgc aattctggcg cggtgctcgc gaggcctgca atccccggag cgagtttccc
     5281 gcgctatgcc tctacgcgcc atgactgtcg tcgaatatcg cagcagacgg tcggacgacc
     5341 agattgagcc gcggcacggc taagcgtagg attccacccg aacattgcca gaggacgagg
     5401 tgctgccgtg aaggtctgca tgttccacct gatgccgtac cgggacctgc cagccgattt
     5461 cgagcggcga tacaactcgg cctacatcga tcccgtgtgg ttcgaggtgg ccgattccga
     5521 caaggttggc cagttctaca acgcgaccct cgacgagatg ctctatgccg ccaaggccgg
     5581 cctgcacggg ctatgcacca accagcatca ccagaacgtc tatggcttca tggcaaatcc
     5641 aagcatcatg ggcgcggtgc tcgcgaggca gaccaatggc cagaatatcg caatcatcca
     5701 gctcggctcg acgctcccct ccaccacgcc gccgacgcgc atcgcggaag aatacgcgat
     5761 gctcgactgc atcagcggcg gacggctggt cgctggattt cccacggggc tgcccgccga
     5821 cgccaccatc tcgaacggcg tcatcccggt cgagcagcgc gagcgtttcc gcgaagcgct
     5881 ggccctagtg attaaggcct ggtctgcgag ggacattttc gcctggaacg gcaggcatta
     5941 tcagctcggc atggtgaacc tttggccgcg accaatccag cagccgcatc cgccgatctg
     6001 ggttccgggc tcaggcatct cctcgaccgc ggagtacgtg gttggccagg accattgctt
     6061 ctgtcatctg agctattacg gcgccaagaa cgcggagcag gtgagcgatc ggtattggga
     6121 gctggtcgcg cgcaaggggc gcgacgacaa tccctatcgc ttcagcttct tgcagctcat
     6181 cggcgtgggc gagaccgacg ccgaggccga ggatctatac gccgaacacg ccgagtattt
     6241 cttccacaag ctgctctata ccccgagcta ctatcaggcg attccggggt gcctcgaata
     6301 cgaggcgctc gtgcagtcgc tgcgtcacaa tccccgcgcc agcatcaatc tgcgcgaact
     6361 caaggccaag gatttgttcg gccgcggttt cgtcgtggtc ggaagcccga agacggtgcg
     6421 cgagcagctc atcgacggca ttaagcgctt gcgcatgggg cacctgctcg cgctcctgca
     6481 tttcggctcc atgccaacta cgctgtgcaa gaagaacatc gatctgtttg cgcgcgaagt
     6541 gctgccgcat ctcgcggacc tgtgggacga caagtacgag gaccgctggt ggcccgaacg
     6601 ccttaaggcc aagcgtcctg ccgccgcctt ggccgcagct tcgtagaagc tggcgggaga
     6661 gtggacatgg ccgccccgga ggtacgcagg ctcagccttt ggcaggaccg aattcaaacc
     6721 gaggtcgaga tcagcggccg cggtcagccg ctcgtgtacc tgcacgggcc gtgggggctt
     6781 gcccccgatc gtgcgttcgt cgccggcctt gccggcggcc atacggtcta tgcgccgaag
     6841 tttccgggga cgactcacgg caatcctgac gcggtacacg cgctcgacaa ctggttcgac
     6901 ctgatcctct attacggaga actgttcgac acgctggagc tatccgtgcc ggcggtggtg
     6961 ggccattcct tcggcgggct cgtcgccgcc gaattcgcgg cgtcggctcc gaactcggtc
     7021 ggccggctgg tgctgatcga tccggtcggc ttgtggcggg acgacgcgcc ggtaaagaac
     7081 tggatgatcc tcagcgacaa ggtgcgacgg ccctcgctct ttgccgacag cgacggagag
     7141 gccgcgcgcc gcttcttcga tgttccgagc gatccggctg agcgcattga tacgctcgcg
     7201 cagttcatct gggcgcaagc ctgttctggt aaattcgtct ggccgatcgc cgaccgcgga
     7261 ttcagcaagc acgctcaccg gattgcggcg ccgacgctca tcgtctgggg cgcggccgac
     7321 ggcatcatcc cgccggttta tgctcaggag tttgctcgac gaatcgccga cgcacaggtc
     7381 gagctcattg accacgccgg ccacttgcca cacctggaac atccggaggc cgtgacgaaa
     7441 gtggtgcgcg cctttcttgg cagctgaaag gcagcatcac tccaagagcc ctcgtcatac
     7501 cttgacggca tagccgccgc gagagcaggc ttgacgcacc cgcccgagag cggggccgta
     7561 gggaaggaag cggaagatgt ccaccgcctc gacagcgtcg gttgccgtga cgcccgtcac
     7621 ggtgagcgac cgatggactc gttacaacgc taccgtgctg acgatctgca tcctcggctg
     7681 ggcgttcgac atctacgaag ccaccatcat gcagctcgtc acgccgatcc tcatcaaaga
     7741 atgggggatc acgccggcga cgatgggcac ggtgaccacg atctcgcgct ggatcgggct
     7801 catcggcacg tttgcctttc cagtccttgc cgatctctat ggccgcaagc cggcgctcat
     7861 ctgggcgatc ttgggctatt cgatctttac cggcctcacc gggttctcga ccggctggat
     7921 cacgctcttg atcttcagtt cgatcacgag gatcgctctc gccggagaga atccggtcgg
     7981 catgttgatg gtcaccgaga cggcgccaac caaatggcgc gccaccgcgt tgggcggcct
     8041 cgtcggcggc tatccgttcg gttacatgct gtgctcgctt gccgcacttg tcgtggtgcc
     8101 gctgtgggga tggcgctcgc tctacttcct cggcatcctg cccgcgctgc tcgtgctctg
     8161 gatccgcatc ggcatcaagg aaagcccacg ctatgagcgc gtcaccgcgc agatgctcaa
     8221 ggaggggctg aagaagcaac tcgatatcct ctcgccggcg cgcgaatacc cgcgcgaaac
     8281 tctgatcgcc tcgctcgtct acttcttcta cctcttcact tggctcggct ggtcggcctg
     8341 gatgccgttc taccttgcga ccgaaaagaa gctcggcttc caggtcatgg gcagctacct
     8401 ctcgatctgg atgttctgcg ccatcttcgc ctattggatt tgcggctggc tgtgcgacgt
     8461 gttcgggcga cgctgggtga tcccggcttt cgcagtcccg gccggcgctc tcctcatcgc
     8521 gatgggccat gttgatagcc cctcgagcct gttctgggtg ggactggcaa cgaactttct
     8581 catcaccggc agcttcggca ccgggcttgg ctatacggct gagctattcc cgacccagat
     8641 tcgcggtacc ggcgtcggcg catccttcac cttcggcctt ggggtcgcct cgctcgcgcc
     8701 gatgatcatg ggctggatcg cgaccgccca ttccgtcgct gctggcctgc cgctgctggc
     8761 atgctcgttt ctgctgttgg caccgctctt catctggttc gcgccggaca cgacccgcaa
     8821 ggaactcacc gatttcgtcg gacagaaagc tggctgatcc cgtaattgca atgattggaa
     8881 cggcggtcta acggcgcatg acgaccgctg aatacagccg atgaccgccg atccagcgtc
     8941 ctccatcgtt gtcaacaacg tccgcgtcga gttgattgaa cgcggtcacg gcaagccgtt
     9001 gctgttcctg catcccgaaa tcggcatcga gccggccgcg ccggtgctcg atcagcttgc
     9061 gagtcgcatg cgcgtgctag cgcccgtcca cccgggcttt ggacgctcgg aacaaccgaa
     9121 ggccttcgat accgtcgacg atcttgcgta tttctacctc gatctgttgg agcatctcga
     9181 tttacgccaa gttaccgtcg taggcgtatc attcggcggc tggattgccg ccgagatcgc
     9241 gatcaagtct accgagcggc tatcacacct tgtccttgcg aatgcagtag gcatcaaagt
     9301 cggtgaccgg gagacgcgtg acatcgttga catctacgcg attccccaga gcgaatatgt
     9361 tcagcttgcg tatttcaatc cggatgtcgg cacccacgac tacaagacca tgccggacgc
     9421 ccgggttctg gccgcagcgc gcaaccggga agcgaccgcg cgctatgctt ggtcgcctta
     9481 catgcatgat cccaagcttc tgggacgcgt ccaccgtatt gacattccga cattggtgct
     9541 gtggggagcg aacgaccgca ttgtgttcga gtcctacgga agggcatatt gtgcagccat
     9601 taccggtgcg cgcttcgagt tgatcgaacg cgcgagccac tttccccatc tggagcagcc
     9661 cgagatcttt gccgatcgca tcttttcgtt cgtcggggag acatcgcaat gagggtgtac
     9721 caattcaccg agcagccata ccctgatgcc tggaaggacc atggggggtc attgcgtgtc
     9781 aatcttccca accgcaaatg cgacccgacg atcgccgccg atctgttcca tcgttattac
     9841 gacgagtggc tgctggccga tgaactcggc ttcgacatca tgctc
//
LOCUS       DATLJT010001587         1640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2453659,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001587 DATLJT010000000
VERSION     DATLJT010001587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453659"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VM910_36385"
     CDS             complement(<1..358)
                     /locus_tag="VM910_36385"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HVI68020.1"
                     /translation="MPAYEPPPKYSEFLQRMLVRARESLGEEFRGITADGIVRPGLFP
                     VRKTGVSLEPILRAARDFLATLDLRQQRRVSFNIQSDVWRSWSNVHPFLMRHGLGLHE
                     LTAAQREAALALVSSAL"
     gene            627..>1640
                     /locus_tag="VM910_36390"
     CDS             627..>1640
                     /locus_tag="VM910_36390"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI68021.1"
                     /translation="METAAQQRPGAQRSGTSPLYQVLAEDIVAMGVEAAFGLISDDTA
                     LLATALDTSGARFYGARHENCAIAMADGYAYAGGRLGVAVIGRGPALANGLHGAIYAS
                     RTGSPVLLIYGEAATISQPNGLGPDYKAIDGPGVLRTAGLRTFSATSPQAARAVLADA
                     VAAAMTGTAVSLHLPTNVQLAEIERPERPLLIRQVERKAAPAKPQSIAAAAALLSKAT
                     QPLIVAGLGAHRAGARKAIEALAEKIGALLVTTARGKDLFRGNLNNLGIIGSFSHSIA
                     RRMADQADCVLVFGASLNFLTTSFGTSLPQVPLIQVDALRTNIGRWFAADVGVVGDAR
                     LVAE"
BASE COUNT          316 a          507 c          521 g          296 t
ORIGIN      
        1 tcagcgcgct gctcactaac gcgagtgcgg cctcgcgttg cgcggcagta agctcgtgga
       61 gccccaggcc gtggcgcatg aggaatggat gcacattgga ccatgaacgc catacgtcgc
      121 tctggatatt gaacgagacc cgacgctgct gtcgcaaatc gagcgtcgcc aggaaatcgc
      181 gtgccgcccg caaaataggt tcgagtgata ccccggtctt gcgcacgggg aacagccccg
      241 gccggacgat tccatcagct gtgatgccac ggaattcctc gcccagcgat tcccgggccc
      301 gcaccagcat gcgctgcagg aattcggaat atttcggggg aggctcatac gccggcatcg
      361 gcaggcggcg ccgaagtggc gggtgctggc gggccggcgc tgggttgagc gaatgcgtgt
      421 aatcgtgact catgtcaacc caattaccta gaccccgcgt gtttcggatg gctcccacac
      481 cgacttctgc tcgaccggca tgaattgcgg acaggtacac acatcgagcg caacagacgg
      541 tctcggatat ggatcataga ctaccgccgc taattgcgcg attaggaata ctgtaaccac
      601 gaaaagcacc gcgacgggag agtgagatgg agacggcagc tcaacaacgt ccaggcgctc
      661 agaggtccgg cacgtccccc ctctatcaag tcctcgcgga ggatattgtg gcgatgggcg
      721 tcgaggcggc ctttggtctt ataagcgatg ataccgctct actcgcgaca gcgctggaca
      781 cttctggggc acggttctac ggcgcacgac atgagaactg cgccatagcc atggctgacg
      841 gatacgccta tgccggcggc cggttgggcg tggccgtgat cgggcgcgga cccgcgctcg
      901 ccaacggcct gcatggcgcg atttatgcga gtcgcacggg ctcccctgtg ctcctcatct
      961 acggcgaggc ggcgacgata agccaaccca acgggttggg cccagattat aaggcaatcg
     1021 atgggcccgg tgtgcttaga acggccggat tgcgcacctt ctcggccacc agtccacaag
     1081 cggcgcgcgc agtgctcgcc gacgccgtag cggctgccat gaccggcact gcggtgagct
     1141 tgcacttgcc gacaaatgtg cagctcgccg aaattgaacg gcccgagagg ccgctgctga
     1201 ttcggcaggt cgaacgaaag gcagctccgg ccaaaccgca atccatcgcg gcggccgcgg
     1261 cgctcctgtc aaaggccacg cagccactca tcgtggctgg gcttggggca caccgtgccg
     1321 gcgctcgcaa ggcgatcgag gcgctcgccg agaagatagg tgcgctcctc gttaccacag
     1381 cacgaggcaa ggatctgttt cgcggcaatc ttaacaacct cggcatcatt ggctcgttct
     1441 ctcattccat tgcgcgtcgt atggcggatc aggctgactg cgtactggtg tttggcgcga
     1501 gcctgaactt tcttaccacg agcttcggaa catcgctgcc gcaggtgccg ctgatccagg
     1561 tcgatgcgct acgcaccaat atcggccgct ggtttgccgc cgacgtgggg gtggtcggcg
     1621 acgcacgtct cgtggccgag
//
LOCUS       DATLJT010001588         3208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1994878,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001588 DATLJT010000000
VERSION     DATLJT010001588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994878"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>3208
                     /locus_tag="VM910_36395"
     CDS             3..>3208
                     /locus_tag="VM910_36395"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI68022.1"
                     /translation="MYLTEDDLKELGLTIGLRRRLLHAIEEGLRRPAAELVPPAPGGP
                     APSANRGAERRQLTILFSDLAASTELASRLDPEEMSVLLRAYQACCIAAIQKYGGQIS
                     RIFGDGILTYFCYPQAHEDDAERAVRAALDIVRDVPRLRPLGDIVLGVRIGIATGQVL
                     IGETIGEGESKQVDVVGETPNLASRLQAQARPNGIVISTSTHRLTEGLFECTSLGKLH
                     LKGFTEPVVVWQVHGESTAESRFDAMHSRALTPMVGRDRELGLLMQCWERVGAGDVQV
                     ALISGEAGVGKSRILNALRAMLNPKGPYILTLYCSPYHQASALHPVINLYERLAGMAH
                     DDSHAQKLAKLETLLVENGAVLEQAVPIVATLLSIPFGDRYATLKLTPEQLKEQTLQL
                     LIRRIGDMSLQKPVLFLVEDAHWIDPTTLELLSRMIPALKTSSVFLIVTFRTPTDNFK
                     LQHETPSLHLPLARLDRQQAEQLLRHNVGGRPMSPKVSAEIIARADGIPLFVEELSKA
                     VMESGLADVDGDDAAVRGASPGGVVPATLHDSLMARLDRMSLVKPVAQFAAVLGRVFT
                     VQLLKAAVPPHWPPIDGAITALASAEIILPVQLGANLAYQFKHALLQDVAYQSLLKTT
                     RRDYHARIAHVIAEQFGDIAETQPEIVARHFTEAELYDEAVAYWLKAGQRSTQRSSNL
                     DAISHFERGLALVELMGDLVERPRTEYKFCLALITPLIATKGYTAPELERLFKRALRL
                     SEEIGDTEEILPVLYSRQAYELVGGQFDRAAAHAEEAIRIAERNPLADSAAFAGRLFA
                     TLKLFQGEATTACEQLRQMLAQYDPVRHSASALRFGQDHFVACASYLTLGLWFLGFTD
                     EARRYSERAIEYARSLNHTHTLQFGLAYAGALFAANCRDVEFLESTTAELLEIGKELG
                     SPGWSAAVSGLRGKLLIERGRTSQGIAKLQAGIIAFRERRSPLWQPTFCTWLAEAHAT
                     CGEISQGLEALKIGRQAAAGGTHWMDAELHRAAGELLQVGEATNPACAETSFIEALAV
                     ARSQSSRTLELRAAMSLARLWKSQERDTEARALLQPATA"
BASE COUNT          599 a          975 c         1023 g          611 t
ORIGIN      
        1 taatgtacct gaccgaggat gatctcaagg agctcgggct tacaatcggt ctgcgccgac
       61 gcctgttgca cgcgatcgag gaaggtttgc gccggcctgc cgcggagttg gtgccgcctg
      121 cccctggtgg gcccgcaccg agcgcgaacc gaggcgccga acggcggcaa ttaacgatcc
      181 tattttcgga tctagcagca tcgacggaac tcgcgtcgcg gttggacccg gaggagatga
      241 gcgttcttct tcgtgcctat caggcctgct gtatcgccgc gatccaaaag tatggcggcc
      301 agatttcgcg gattttcggc gacggcatcc tcacatactt ttgctacccg caggcgcacg
      361 aggacgatgc cgagcgcgcc gttcgcgccg cccttgacat cgtgcgcgac gtcccgcgcc
      421 tacgcccgct cggcgacatc gtgcttggcg tgcggatcgg gatcgccaca gggcaggtct
      481 tgatcggcga gacgatcggc gagggcgagt ccaagcaggt cgatgtcgtt ggcgagacgc
      541 caaatcttgc ctcgcggctg caagcccagg cgcggccgaa cggcatcgta ataagtacaa
      601 gcacgcaccg cctcaccgag gggttgttcg agtgcacaag cctgggcaaa cttcatctga
      661 agggcttcac cgagcctgtc gtcgtgtggc aggtgcatgg cgagagcacg gccgagagcc
      721 ggttcgacgc gatgcattcc cgggcgttga ccccaatggt tgggcgcgac cgcgaacttg
      781 gcctgctgat gcaatgttgg gaacgcgtcg gcgccggcga tgtgcaagtg gcgctaatct
      841 cgggcgaagc gggtgtcggc aagtcgcgca tcctgaacgc gctgcgcgcc atgctgaacc
      901 ccaaagggcc gtacattctg acgctatact gctcgccata ccatcaggcg agtgcgctgc
      961 atccggtgat caacctttac gagcgcctcg cgggcatggc gcacgatgat tcccacgccc
     1021 agaagctcgc caagctggag accttgttgg ttgagaacgg ggcggtcctg gaacaggccg
     1081 tgccgatcgt ggcgaccctg ctgtcgattc cgttcggcga ccgttatgcg acgctaaagc
     1141 tgacgccgga gcagctgaag gaacagaccc tgcagctgct cattcgccgg atcggggaca
     1201 tgtcgctgca aaagccagtt cttttcctcg tcgaggacgc gcattggatc gatccgacga
     1261 cgttggaact gctctcgcgc atgatccccg cgctgaaaac atccagcgta ttcttgatcg
     1321 ttacgtttcg cacgcccaca gacaatttca aactgcagca cgagacgccg tcattgcacc
     1381 tgccactcgc ccggctcgac cgtcagcagg cggagcagct tttgcggcac aacgtgggcg
     1441 gccggccgat gtcgccaaag gtgagtgccg agatcatcgc gcgcgcagac ggcattccgc
     1501 tgtttgtcga ggaattgagc aaggccgtga tggagtcggg cctggcggac gtggacggcg
     1561 acgatgctgc ggtgcgcggc gcgtcgccgg ggggcgtggt tccggcgacc ctgcacgact
     1621 cgcttatggc tcgtctcgac cgcatgtcac tggtcaagcc ggtcgcgcag ttcgccgccg
     1681 tgctcgggcg tgttttcacc gttcagctcc tcaaggccgc agttcctccg cactggccgc
     1741 caatcgatgg cgcaatcacc gctctcgcct cggccgaaat catcctgccc gttcagctcg
     1801 gcgcaaacct cgcctatcag ttcaagcatg ccctgctgca ggacgtcgcc taccagtctc
     1861 ttctgaagac gacgaggcgc gactatcacg cgcggatcgc ccatgtgatt gccgaacagt
     1921 tcggcgatat cgccgaaacg caacccgaga tcgtcgcccg ccatttcact gaggccgagt
     1981 tgtacgacga ggcagtcgcc tattggctga aggcgggaca acggtcgacc cagcggtcat
     2041 ccaatctgga cgcgatatcg catttcgagc gcggcttggc gctggtcgag ctcatgggag
     2101 atttggtcga gcgtccacgg acagagtaca aattctgctt ggcactcatc acaccgctaa
     2161 ttgcaactaa gggttacacg gcgcccgaac tcgaacggtt attcaaacgt gcgcttcgac
     2221 tgagcgagga gatcggtgat accgaggaga tcctccctgt cctgtacagc cgacaggcat
     2281 acgagctcgt cggcgggcaa ttcgaccgcg cggccgcgca cgccgaggag gcgattcgaa
     2341 tcgccgagcg taaccccctc gccgattccg ctgcttttgc cggcaggctc ttcgcaacac
     2401 ttaagttatt tcagggggag gcgacaaccg cctgcgagca actgaggcag atgctcgccc
     2461 aatacgatcc ggtgcggcat agtgcatcgg cgcttaggtt cggccaggat cacttcgttg
     2521 catgtgcgtc ttatctgact ttagggctgt ggtttctcgg tttcaccgac gaggcgcgac
     2581 ggtatagcga acgcgccatc gagtacgcgc gatcgctgaa ccacacccac acgctccaat
     2641 tcggtcttgc atacgcgggc gccctatttg cagcgaattg ccgtgacgtg gagttcctgg
     2701 aatcgacgac cgcggaactg ctggagattg gcaaggagtt ggggtcgccg gggtggagcg
     2761 cggccgtcag cggattgcgt ggcaagctcc tgatcgagcg cggacggacg agccagggta
     2821 ttgccaaatt gcaggccggc atcatagcgt ttagggagcg tcgttcgcca ctctggcagc
     2881 ccacgttctg cacctggctt gcagaggctc atgcgacctg cggcgagatc tcgcaggggc
     2941 tcgaggcgtt gaagatcggc cggcaagctg cggccggagg aacccactgg atggatgcgg
     3001 aattgcatcg cgcggcgggc gaacttctgc aggttggaga ggcgactaat cccgcctgtg
     3061 cagaaacgag ttttattgag gctctcgcgg ttgccagatc gcaatcgtca cgaacgctgg
     3121 aattgcgtgc cgcaatgagc cttgcgcggt tgtggaaaag ccaggagcgg gacacagagg
     3181 cgcgtgcctt gctgcagcct gccacggc
//
LOCUS       DATLJT010001589         2214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2224217,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001589 DATLJT010000000
VERSION     DATLJT010001589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224217"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VM910_36400"
     CDS             complement(<1..191)
                     /locus_tag="VM910_36400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68023.1"
                     /translation="MAARPGGRADIAACACAAVRFARHRCASRPIEDPEVTTLNLDGE
                     IRDGRHHMQIRVYYEDTDFS"
     gene            complement(219..1262)
                     /gene="ruvB"
                     /locus_tag="VM910_36405"
     CDS             complement(219..1262)
                     /gene="ruvB"
                     /locus_tag="VM910_36405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVI68024.1"
                     /translation="MNQPSRIVTPERRNEDTGDTALRPQLLSEFVGQQQARANLAVFI
                     EAARKRGEALDHVLFVGPPGLGKTTLAQIVARELGVGFRATSGPVIAKAGDLAALLTN
                     LEERDVLFIDEIHRLSPAVEEVLYPAMEDFQLDLIIGEGPAARSVKIELAKFTLIGAT
                     TRAGLLTNPLRDRFGIPVRLNFYTEEELEKIVSRGARVLKVGITEDGANEIARRARGT
                     PRIAGRLLRRVRDFASAANAASIDRKIADHALSALEVDAAGLDAMDRRYLSTIAMNYG
                     GGPVGVETLAAALSEPRDAIEDIIEPYLIQCGYLQRTPRGRLLTSHAFRHLGLAEPAR
                     DPAQFGLFGDSED"
     gene            complement(1259..1876)
                     /gene="ruvA"
                     /locus_tag="VM910_36410"
     CDS             complement(1259..1876)
                     /gene="ruvA"
                     /locus_tag="VM910_36410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HVI68025.1"
                     /translation="MIGKLKGLIDSYGEDFLILDVGGVGYQVHCSARTLQALPAPGQA
                     AVLSIETYVREDQIKLFGFRSDSEREWFRLLQTVQGVGAKVALAVLGTLPPAELANAI
                     ALRDKAAVTRTPGVGPKVAERIVTELKDKAPAFANVDPAVVSLSGALDDQRAPRPVTD
                     AISALVNLGYGQPQAAAAIAAASRDAGEHAGVTQLIRLGLKELAR"
     gene            complement(1873..>2214)
                     /gene="ruvC"
                     /locus_tag="VM910_36415"
     CDS             complement(1873..>2214)
                     /gene="ruvC"
                     /locus_tag="VM910_36415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVI68026.1"
                     /translation="AVLRDFKPAEAAIEQTFVNKDGVATLKLGQARGVAMLTPAMFGI
                     QIAEYAPNLVKKTVVGVGHADKNQVAMMLKLLLPKAEPTSPDAADALAIAITHAHHRQ
                     SATLRLKVVGI"
BASE COUNT          373 a          720 c          733 g          388 t
ORIGIN      
        1 gaaaaatccg tgtcctcgta gtagacgcgg atctgcatgt ggtggcggcc gtcgcggatc
       61 tcgccgtcga gattgagtgt cgtcacctct gggtcctcga ttggcctcga agcgcatcgg
      121 tgtcgcgcaa atctgacggc cgcgcaagcg catgctgcga tatcggcgcg gccgccgggc
      181 ctcgccgcca cgggcatggc ccgggcggaa cgaaagcctc aatcttcgct gtcgccgaac
      241 aggccgaact gcgccggatc gcgtgcgggt tctgcgagtc cgagatggcg gaaggcgtgc
      301 gaggtcagga gacgtccgcg cggggtgcgc tgcaggtagc cgcactggat cagatacggc
      361 tcgatgatgt cctcgatggc atcgcgcggc tccgatagag cggcggccag tgtctcgacg
      421 ccgaccggtc cgccgccata gttcatcgcg atcgtcgaga ggtagcggcg gtccatcgca
      481 tcgagacctg ccgcatcgac ctcgagcgca ctgagcgcat gatcggcgat cttgcgatcg
      541 atcgaagccg cgttggcggc agaggcaaag tcgcgcacgc gtcgcaacag ccggccggca
      601 atgcgcggcg tgccgcgcgc gcgacgcgcg atctcgttgg cgccgtcctc ggttatcccg
      661 accttcagca cgcgggcacc gcggctgacg atcttttcca gctcttcctc ggtatagaaa
      721 ttgagccgca ccggaatgcc aaaccggtcg cgcagcggat tggtcagaag ccctgcccgc
      781 gtcgtggcgc cgatcagtgt aaacttcgca agctcgatct tgaccgagcg ggctgcgggt
      841 ccctcgccga taatgagatc gagctggaaa tcctccatcg ccggatagag cacttcttca
      901 accgccgggc tcaggcgatg aatttcgtcg atgaagagta cgtcgcgttc ctcgagattg
      961 gtgagcagcg cggcgagatc gcccgccttc gcgatcaccg gccccgaagt ggcgcgaaag
     1021 ccgacgccaa gctcgcgcgc cacgatctgc gcaagcgtgg tcttgccgag tccgggaggg
     1081 cccacaaaca gcacatggtc caacgcctcg ccgcgctttc gcgcggcttc gatgaagacg
     1141 gcgagattgg cgcgcgcctg ctgctggcca acgaactcgg acaagagttg cgggcggagc
     1201 gcggtatcgc cggtatcctc gttgcgtcgc tcgggggtca cgatgcggga gggttggttc
     1261 acctcgcgag ctccttaagg ccgagccgga tgagttgcgt cactcccgca tgctcgccgg
     1321 catcgcgcga ggcggccgcg atcgcagccg ccgcctgcgg ctgcccatag ccgagattga
     1381 ccagcgcgga gatcgcatcc gttaccggac gcggtgcgcg ctgatcgtcg agcgcgccgg
     1441 agagactgac aaccgcggga tcgacattgg cgaaagccgg ggccttgtcc ttcagttcgg
     1501 tgacgatgcg ctcggcgacc ttcgggccga cgccgggcgt ccgcgtcacg gcggccttgt
     1561 cgcgcagcgc gatcgcgttg gcaagttctg ccggcggcag cgttccgagc acggcaaggg
     1621 ctaccttggc gccgacgcct tgcaccgtct gcaacaggcg aaaccattcg cgctcgctgt
     1681 cgctcctgaa gccgaacagc ttgatctgat cctcgcggac ataggtctcg atcgagagca
     1741 ccgcggcctg cccgggtgcg ggcagcgcct gcagcgtgcg cgccgagcag tgcacctgat
     1801 agccgacgcc gccgacgtcg aggatgagaa aatcctcgcc gtaagaatcg atcagtccct
     1861 tgagcttgcc gatcatattc cgacaacctt cagccgcagc gtcgcgctct ggcgatggtg
     1921 ggcatgggtg atcgcgatcg cgagcgcgtc ggctgcgtca ggcgatgtcg gctcggcttt
     1981 cggcaacagc agtttcagca tcatcgcgac ctggttcttg tcggcatgtc ccacaccgac
     2041 cacggtcttc ttcaccaggt tcggcgcata ttcggcaatc tgaatgccga acattgcggg
     2101 cgtcagcatg gcaacgcccc gcgcctgccc gagcttcagc gtggcaacgc cgtccttgtt
     2161 gacgaaggtc tgctcgatgg cggcttcggc cggcttgaaa tcccgcaaca ccgc
//
LOCUS       DATLJT010001590         1591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4291283,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001590 DATLJT010000000
VERSION     DATLJT010001590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291283"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VM910_36420"
     CDS             <1..246
                     /locus_tag="VM910_36420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68027.1"
                     /translation="KLGFAVFEITDNGRGIDPKDHQRIFELFRRAGTQDRPGQGIGLA
                     HVRALVRRLGGTMSVSSELNCGSTFVVTLPLSWKFES"
     gene            267..707
                     /locus_tag="VM910_36425"
     CDS             267..707
                     /locus_tag="VM910_36425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68028.1"
                     /translation="MTTPVTIIMIEDDDGHARLIERNIRRSGVNNKIVPFTNGTEAVR
                     YLFGKDGSGAERKDDALLILLDLNLPDMTGIDILRQVKGNPYLKCSPVVVLTTTDDSQ
                     EIKRCYELGCNVYITKPVNYESFANAIRQLGLFFSVIQVPSTAK"
     gene            704..>1591
                     /locus_tag="VM910_36430"
     CDS             704..>1591
                     /locus_tag="VM910_36430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68029.1"
                     /translation="MTQPKPTLLYIDDDEALARLVERGLTRLGLNVVHAPGGAEGLDR
                     LKQGGIDVVALDQYMPGLDGLETLERILAIPNAPPVVFVTASQDSNIAVTALKAGAAD
                     YLVKDTLGDFVPLLQVAIDGALRQAELQRARDEAEAEVHASRDRYAALAAERELLLRE
                     VNHRVGNSLQIIASLLHLQANSSTQDDVKAALTNAMGRVAAVAQVHRRLYTSHDLKSV
                     LLNQYLDALLEDLRRSTEGERMSRLTLKAEPIEIDPDRAVAIGIIVNELVMNAVKYAY
                     TDCTGPIHVALTSDGDDLLL"
BASE COUNT          316 a          516 c          476 g          283 t
ORIGIN      
        1 aagctcggct ttgcggtgtt cgagatcacc gacaacgggc gcggcatcga tcccaaggac
       61 caccagcgca tcttcgagct gtttcgccgc gccggaaccc aggacagacc cggtcagggc
      121 atcggcctcg cccacgtgcg cgcgctggtg cggcggctcg gtggaaccat gtcggtctcg
      181 tcggaactta attgcggcag cacctttgtc gtaaccctcc ccctcagttg gaaatttgaa
      241 tcctgaacaa tcggaacggt acagtgatga ccacgcctgt cacaattatc atgatcgaag
      301 acgacgacgg ccacgcgcgc ctgatcgagc ggaatattcg ccgctccggc gtgaacaata
      361 aaatcgtgcc gttcaccaac ggtacagaag cggtgcgcta tctgttcggc aaggacggca
      421 gcggcgcgga gcgcaaggac gacgcgcttt tgatcctgct cgatctgaat ttgccggaca
      481 tgacgggcat cgacattctg cgccaggtca agggtaaccc gtacctgaaa tgcagtccgg
      541 tcgtggtcct caccacgacc gatgattccc aggagatcaa gcgctgctac gaactcggct
      601 gcaacgtcta catcaccaag ccggtcaact atgagagctt cgccaatgcc attcgccagc
      661 tcggactgtt cttctctgtg attcaggttc cttcgacggc caaatgacac agccaaaacc
      721 cacgctgctc tatatcgacg acgacgaggc gctcgcgcgc ctggtggagc gcggcctgac
      781 ccggctcggc ttgaatgtcg tgcacgcacc cggcggggcc gaggggctcg atcggctcaa
      841 gcaaggcggt atcgacgtgg tcgcgctcga tcagtacatg cccgggctcg acggcctgga
      901 aacactcgag cggatcttgg ccatcccgaa cgcgcctccg gtcgtgttcg tcacggcatc
      961 gcaggattcg aacatcgcgg tcaccgcgtt gaaggcgggc gcagccgatt atctcgtcaa
     1021 ggatacgctg ggcgatttcg tcccgctgct gcaggtcgcc atcgacggcg cgctccgcca
     1081 ggccgaattg caaagagccc gcgatgaggc ggaggccgag gtgcacgcct cacgcgaccg
     1141 ctatgcggcg ctcgccgccg aacgcgaact gctactgcgc gaggtcaacc atcgcgtagg
     1201 caactccctg cagatcattg cctcgctcct gcacctgcag gccaactcgt cgacccagga
     1261 cgacgtcaag gcggccttga ccaatgccat ggggcgcgtc gcggcggtgg cgcaggtcca
     1321 tcgccggctc tacacctcgc acgatctgaa gagcgtgctg ctgaaccagt atctcgacgc
     1381 gctgcttgaa gatctgcgcc gctcgaccga gggcgagagg atgtcgcgcc tgacattgaa
     1441 ggccgaaccg atcgagatcg atcccgaccg cgcggtcgcg atcgggatca tcgtcaacga
     1501 gctcgtgatg aacgcggtca aatacgctta caccgattgc accggcccga tccatgtcgc
     1561 gctcaccagc gatggcgacg acctcctgct g
//
LOCUS       DATLJT010001591         4205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2913598,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001591 DATLJT010000000
VERSION     DATLJT010001591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913598"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VM910_36435"
     CDS             <1..793
                     /locus_tag="VM910_36435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68030.1"
                     /translation="YVLATIAVFGFALLGIVAARSTAATLLAGAFGCLAIYLMINPSK
                     ASYSMAPTMVACALAGFLTAHWLKPGKHDYNIGLTLLLGFVLGLSVDFRLPNLFLSSG
                     YFLFFLVAFLTSRRASLVMQGALFALAFLTGIAPTLVSNAINAGSPLATTYGGQDVTA
                     PAFSFGIVWQYLADMQFVLLALSIVGTAYLLRSRDEGAWRVALLTAMNLAVNLAFFLS
                     HPVFTPYYTIPIAMLSLWSLSFAALIQPAEVVDDRLAGQAVRVRS"
     gene            942..1871
                     /locus_tag="VM910_36440"
     CDS             942..1871
                     /locus_tag="VM910_36440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVI68031.1"
                     /translation="MTSTSKRIAVLVPCFNEEAAVANVVTGFRKALPTAQIYVYDNNS
                     RDRTAAVARAAGAEVRSERRQGKGHVVRRMFADIDADVYVLVDGDATYDAPSAPRMIE
                     KLVDEHLDMVVGLRVDQEQAAWRRGHRIGNRMLTGFLSSVFGQAFKDILSGYRVFSRR
                     FVKSFPVLSDGFEIETELNVHALELAMPVAEVETPYYARPEGSVSKLNTYRDGFRILG
                     TILKLYRSEKPLRFFAVIGVFLALVSIGLAIPIVITYLEEGLVPRLPTAVLSTGLMIV
                     AVLSVSSGLVLDTVTRGRREMKLLAYLSQPPVS"
     gene            2064..2576
                     /locus_tag="VM910_36445"
     CDS             2064..2576
                     /locus_tag="VM910_36445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVI68032.1"
                     /translation="MNDLSITILPETANDAQAIERLHERTFGPGRFVLSAYRLREHVD
                     HLRDLSYTAHIGTLLVGSVRQLPVCIGETKALLLGPLTVEPPFRGRGVGNALLRRALD
                     DARARGHRLVLLIGDEQYYGRVGFKRVPKGRATMPGPVDYNRLLIAELVDGAFEGVSG
                     AIRPDWSMAK"
     gene            complement(2598..>4205)
                     /locus_tag="VM910_36450"
     CDS             complement(2598..>4205)
                     /locus_tag="VM910_36450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68033.1"
                     /translation="DIPANAAAVGFQAPVQALITGRKDERDRRIAISAAPRFGIVGQT
                     QTVTYRLDDQGVTGERARIVIRRDGEVISERTLTSGQTSSVDIDIKHAGPNIVEIEAS
                     PLAGELTPVNNRAVVAIDGVRDKLRVLLVSGEPHSGERTWRNLLKSDASVDLVHFTIL
                     RPPEKQDGTPINELSLIAFPTRELFQQKINEFQLIIFDRYARQGVLPIAYFDNIARYV
                     RQGGAVLVSAGPDYASSTSIWRTPLDSVLPAEPVGVTEKPFYAHLSDAGKRHPVTRGL
                     EGAATEPPHWSRFFRTVDTRNAIGPPVMTGADGKPLLLLSRFGEGRVALLLTDHIWLW
                     ARGYEGGGPHLDLLRRTSHWLMKQPDLDEEALRLQVQGKDLHVIRQTMADSVLPVTVT
                     SPSGATRELTLTAGEPGEWRATLPANELGLWQATDGTLKALINVGPTNPKEFSEVTST
                     TEMLKPLAQATGGDAKRVVDGGSVELPRIVGVRASSVFHGDGWMGVKMREASIVKGVG
                     VLPIFAGLIGLLLLLGAFAATWLREGR"
BASE COUNT          669 a         1332 c         1369 g          835 t
ORIGIN      
        1 ctatgtgctg gcgacgatcg ccgtgttcgg ctttgcgctg ctcgggatcg tcgcggcgcg
       61 atcgacggct gcaaccctgc tcgccggcgc attcggttgc cttgcgatct atctgatgat
      121 caatccgtcg aaggcaagct actcgatggc tccgaccatg gtggcgtgcg cgctcgcggg
      181 ctttctgacg gcacattggc tgaagccagg caaacatgac tacaacattg ggctgacctt
      241 gctgctggga tttgtgctcg ggctctcggt cgacttccgg ctgccgaacc tgtttctgtc
      301 gtcaggatat ttcctgttct tcctggtcgc gttcctgacc tcgcgcaggg cctcgctggt
      361 catgcagggc gcactgttcg cgctcgcctt cctcactggg atcgcgccga cgctggtttc
      421 gaacgcgatc aacgccggaa gtccccttgc caccacctat ggcggtcagg acgtgaccgc
      481 acccgccttc tccttcggca tcgtctggca gtatctggcc gacatgcagt tcgtgctgct
      541 ggcgctttcg attgtcggga cggcctatct gttgcgctca cgcgacgaag gcgcctggcg
      601 cgttgcgctg ttaacggcga tgaacctggc cgtaaatctc gccttctttc tgagccatcc
      661 cgtcttcaca ccctattaca cgattccgat cgccatgctt tcgctgtgga gcctgtcgtt
      721 tgccgcgctg atacagccgg cggaagtcgt ggacgaccgg cttgccgggc aggcggtgag
      781 ggtccgatca tgatatggac gccagggcgc ggtagcttct tgacgaatcg ccacagcgcg
      841 tcggctgatt gttttgcgca ggaactttcc ggaaagccgg tgcccgcttt tccggatcgt
      901 gcgctggagt cggcaggctt attcggacag gcgttttcct tatgacatcc acatcgaagc
      961 ggattgccgt cctcgtgccg tgcttcaatg aggaagctgc ggtggcaaac gtcgtcacgg
     1021 gtttccgcaa agcgttgccc acggcgcaaa tctacgtcta cgacaacaat tcgcgggacc
     1081 gcaccgccgc ggttgcgcgc gcggccggcg ccgaggtgcg cagcgagcgc cgccagggca
     1141 agggccatgt ggtgaggcgc atgttcgccg acattgatgc cgacgtctac gtgctggtcg
     1201 atggcgatgc gacctacgac gcgccgagcg cgccgcgcat gatcgagaag ctggtggacg
     1261 agcatctcga catggtggta ggcctgcgcg tcgaccagga gcaggctgcc tggcgccggg
     1321 gccaccgcat cggcaaccgc atgctgacgg gattcctgtc gtcggtgttc ggccaggcct
     1381 tcaaggatat tctgtccggc tatcgcgtgt tctcgcggcg tttcgtcaaa tccttcccgg
     1441 tgttgtcgga cggtttcgag atcgagaccg agctcaatgt tcacgcgctc gagctggcga
     1501 tgccggttgc cgaggtggag acgccctatt acgcgcggcc cgaaggttcg gtcagcaagc
     1561 tcaacacgta ccgcgatggc ttccgcattc tcggcacgat cctgaaactc tatcgttcgg
     1621 agaagccgct gcgtttcttt gcggtgatcg gggtgttcct ggcgctggtc tcgatcggcc
     1681 tcgcgatccc gatcgtcatc acctatctgg aggaaggcct cgtgccccga ttgccgaccg
     1741 cggtgctgtc gacaggcctg atgatcgtcg ccgtgctgtc ggtgtcttcg ggcctcgtgc
     1801 tcgacaccgt gacgcgcgga cggcgggaga tgaagctgct cgcctatctt tcccagccgc
     1861 cggttagcta aatttgatga gatcttgcgg gatgcgcagt acacgtcacg ttaagcagcc
     1921 ggctcatgtc ttcagccgaa ccacaccaga taccatctcg tgagcttcgg ttctggcttg
     1981 aatcggaagc gaagctctga tttttttgac gtgttttctt gaggcgaacc ggtacccgct
     2041 tcgcttggaa acgccatggg atcatgaacg acctttccat caccatcctg ccggagactg
     2101 cgaacgacgc ccaggcgatc gaacggctgc atgagcgcac gttcggaccc gggcgcttcg
     2161 tgctgagcgc ctatcggttg cgcgagcatg tggaccattt gcgcgatctt tcttacaccg
     2221 cgcacatcgg cacgctgctc gtgggatcgg tgcggcaact gccggtttgc atcggcgaaa
     2281 ccaaagcgtt gttgttgggg ccgctgacgg tcgagccgcc gtttcgcggc cgcggcgtcg
     2341 gcaacgcgct gctccggcgc gcactcgacg acgccagggc caggggccac cgcctcgtgc
     2401 tgctgatcgg tgacgagcag tattatggcc gcgtcggctt caagcgcgtg ccgaagggca
     2461 gggcgacgat gccggggccg gtcgattaca atcgcctgct gatcgccgag ctcgtcgacg
     2521 gagcgtttga gggggtctcc ggcgcgatcc gtcctgactg gagcatggcg aagtagcgca
     2581 tcatccggga atgcaggtta ccgcccttcg cgcagccagg tcgccgcgaa cgcgccaagc
     2641 aacagcaaga ggccgatcag gccggcaaag atcggcagca ctccgacgcc cttgacgatg
     2701 ctggcctcgc gcatcttcac gcccatccag ccgtcgccgt gaaacacgct ggaagcgcgc
     2761 accccgacaa tgcgcggcag ctcgacgctg ccgccatcga ccacgcgctt ggcatcgcct
     2821 ccggtggcct gcgccagcgg cttcagcatc tcggtcgtgg aggtgacttc cgaaaattcc
     2881 ttgggattgg tcgggccgac attgatcagc gccttgagcg tgccgtcggt tgcctgccaa
     2941 aggccaagtt cgttcgccgg gagggttgcg cgccattcgc cgggttcgcc tgcggtgagc
     3001 gtcagctcgc gcgtggcgcc tgacggcgag gtcaccgtca ccggcaaaac gctgtcggcc
     3061 atggtctggc ggatcacgtg gagatccttg ccctgcacct gcagccgcag cgcctcctcg
     3121 tcgagatccg gctgcttcat cagccagtgc gaagtgcgcc gcaacaggtc gagatgcggc
     3181 ccgccgccct cgtagccgcg tgcccacagc cagatgtggt cggtcaacag cagcgcgacg
     3241 cggccctcgc cgaaccgcga caacagcagg agcggcttgc cgtcggcgcc ggtcatgaca
     3301 ggagggccga tcgcgttgcg cgtatcgacg gtgcggaaga accggctcca atgcggcggc
     3361 tcggtcgccg ctccctcgag cccgcgcgtc accggatggc gtttgccggc atccgaaagg
     3421 tgcgcataaa acggcttttc ggtcacgccg accggttcgg ccggcagcac cgagtcgagc
     3481 ggggtacgcc agatcgaggt gctggaggcg tagtcgggac ccgccgacac caggactgca
     3541 ccgccctggc gcacatagcg cgcgatgttg tcgaaatagg cgatcggcag cacgccctgg
     3601 cgggcgtagc ggtcgaagat gatcaactga aattcgttga tcttctgctg gaacaattcg
     3661 cgcgtcggaa acgcgatcag cgacagctcg ttgatcggcg tgccgtcctg cttctccggc
     3721 ggacgcagaa tggtgaagtg cacgagatcg acgctggcat ccgacttcag cagattgcgc
     3781 caggtgcgct cgcccgaatg cggctcgccg gataccagca gcacccgcag cttgtcgcgt
     3841 acgccgtcga ttgcgaccac cgcgcggttg ttgaccggcg tcagctcgcc ggcgagcgga
     3901 gaggcctcga tctcgacgat gttggggccg gcatgcttga tgtcgatgtc gacgctcgag
     3961 gtctggccgc tggtcagcgt tcgctcgctg atcacttcgc cgtcgcggcg gatcacgatc
     4021 cgcgcgcgct cgccggtgac gccctggtcg tcgagccggt aggtgacggt ttgcgtctgt
     4081 ccgacgatgc caaagcgcgg cgcggcggaa atcgcgatgc ggcggtcgcg ctcgtccttg
     4141 cgcccggtga tcagcgcctg caccggcgcc tggaagccga cggcggcggc atttgcggga
     4201 atgtc
//
LOCUS       DATLJT010001592         2470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2685773,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001592 DATLJT010000000
VERSION     DATLJT010001592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685773"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VM910_36455"
     CDS             <1..320
                     /locus_tag="VM910_36455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68034.1"
                     /translation="ATLYDKLNHNFIRDIAPVAGIIVVPNVMVVNPSVPAATVPEFIA
                     YATSKPGKTTMASAGTGTPSHVTGELFKMMAGVNLVHVPYRGSVNREKMLRFQCAARE
                     VSQ"
     gene            complement(499..1467)
                     /locus_tag="VM910_36460"
     CDS             complement(499..1467)
                     /locus_tag="VM910_36460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68035.1"
                     /translation="MIRNSVICCGLAVALTVSGPAWGQDYPNRQITILVGIAAGGITD
                     VTTRQYAAVVSKNVDQNIIIENRPVAGGAVAAAAVQSAAPDGYTLLSVVGSQFSSVPA
                     MGPAPYDPVKGFAPISLTFRLPTLLVVPMDSPAKTVAELLAWGKTKPGGLLLGSPGAG
                     TPGHLLAAKIALATSTPIQYIHYRGGSPVMADLIAGRVDFSLASFNSARSNMHAKKLR
                     ALAVDAETRLPALPDIPTLIEAGLGAQRVADWAGVLAPVGTPTAIVDRLNREFVKAAH
                     TPELIAKLTQNGNIVASSTPQEMSRLVADEVKNMEQLIKTLGLKAQ"
     gene            complement(1632..2036)
                     /locus_tag="VM910_36465"
     CDS             complement(1632..2036)
                     /locus_tag="VM910_36465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68036.1"
                     /translation="MPDLLGGRLTMVFSSPVSALPLAREGKVRPLAVTSLTRAPSSPN
                     LPTMAEAGFPGFDATAWFAPLAPAGTPEPIIAKLHREAVRILVLPDVRKRFDELGMVP
                     MGTSPAQFAAAMASEARLWAKVIEASGAKLMD"
     gene            complement(2036..2269)
                     /locus_tag="VM910_36470"
     CDS             complement(2036..2269)
                     /locus_tag="VM910_36470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68037.1"
                     /translation="MSVSQPILDTLTDALDRTLDDDNVHRRPLDLGGNVPSKAKRGQQ
                     PLAALMKGEITCWTWKRMEAYGKRRFPTAASRQ"
     gene            complement(2266..>2470)
                     /locus_tag="VM910_36475"
     CDS             complement(2266..>2470)
                     /locus_tag="VM910_36475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HVI68038.1"
                     /translation="NSGWLVGAGIEWAFANHWSAKLEYDYLGLGSQTFTGPAGSPFLA
                     NDVFTVSNRNIQMVKVGINYLLN"
BASE COUNT          457 a          753 c          790 g          470 t
ORIGIN      
        1 acgcgacgtt gtacgacaag ctcaaccaca atttcatccg cgacatcgcg ccggtcgccg
       61 gcattattgt cgtgcccaac gtcatggtgg tgaatccatc ggtcccggcc gcgacggtcc
      121 ccgagttcat cgcttacgct acgagcaagc cgggtaagac caccatggcg tcggctggaa
      181 ccggaacacc gtctcatgtg accggcgagt tgttcaagat gatggccggg gtgaacctcg
      241 tgcacgtgcc gtatcgcggc tcggtaaatc gagaaaaaat gttgcgattt caatgcgctg
      301 cgcgtgaagt gtcccaatag tgtccagaac aacaaacaag ccctgtggac aagccgattt
      361 ttcctgtgcg cattaacgag ggccgcaaac gagcccgaca gaatggcatt cgcttcggcc
      421 gcaagacacc cctcattcgc ctgagtgtgc gtcgttgcgc tctccgagtg agttcgaggt
      481 ttcagagggt tgcggccatc actgcgcctt taggccaagg gttttgatca attgctccat
      541 gttcttcacc tcgtcagcca caagcctgct catctcttgc ggcgtactgc tggcgacaat
      601 gttgccgttt tgggtcaact tcgcgatcag ctctggagta tgggccgcct tcacgaattc
      661 ccgatttagc ctgtcgacga tggcggtggg ggttcccacc ggcgccaaca cgccagccca
      721 gtcggcgacc ctttgcgcgc cgagcccggc ctcgatcaag gtcggaatgt caggcagcgc
      781 gggaagccgc gtctcggcgt ccaccgcgag cgcccgtagc ttcttggcgt gcatattcga
      841 ccgggcgctg ttgaaggagg cgaggctgaa atccacgcgc ccggcgatga gatctgccat
      901 caccggcgag ccgccgcgat aatggatgta ttgaatcggc gtgctggtgg cgagcgcgat
      961 cttggcggcg agcagatgtc cgggagtgcc cgcgccggga gagccaagca gcaggccgcc
     1021 gggcttcgtt ttcccccagg cgagtagctc ggccacggtc tttgccgggc tgtccatggg
     1081 cacgacgagc agagtcggaa gccgaaatgt caagctgatc ggagcaaagc ctttcaccgg
     1141 atcgtaggga gcgggcccca tggccgggac tgaggaaaac tgcgagccga ccaccgacag
     1201 cagcgtgtat ccatccgggg ctgcactctg aacggcagca gcggcgaccg cgccgccggc
     1261 gaccggacga ttttcgatga tgatgttctg gtcgacattt ttcgacacga ccgccgcgta
     1321 ttgccgggtg gtgacgtcgg tgatgccgcc ggccgcaata ccgaccagga tggtgatttg
     1381 ccggtttgga tagtcctgcc cccaggcggg accggacaca gtcagggcga ctgcgaggcc
     1441 gcagcagatc acggaattcc gaatcatggt tttcctctct gcgaatgaga ctctggatct
     1501 tggcaattgc ggaagcgcat ggatgtgcaa agatttggcc ctgtccccgc ccgtcacgtt
     1561 gcgtcacgcc cggtccacga acacgcagga atcgcaggcg cgtgcgggcc gcgctgaccc
     1621 aaccttgcgc ctcaatccat cagcttggct cccgaggcct cgattacctt ggcccagagc
     1681 cgggcctccg aggccatagc ggcggcgaac tgcgcaggcg acgtgcccat cggcaccatg
     1741 ccgagctcgt cgaagcgctt gcgaacatcg ggcagcacca ggatgcgcac cgcctcgcga
     1801 tggagcttgg caatgatcgg ctccggcgtg ccggccggcg ccagcggtgc gaaccaggca
     1861 gtggcgtcga agcccgggaa gcctgcttcg gccatggtcg gcaagttggg cgatgacggc
     1921 gcgcgcgtga gcgaggtgac cgcgagcggg cgcaccttgc cttcgcgggc gaggggcagc
     1981 gccgagacgg gactggagaa caccatggtc aggcgaccgc cgagcaggtc cggcatcact
     2041 gccgcgatgc cgcggtaggg aaccttcgct ttccataggc ttccatgcgc ttccaggtcc
     2101 agcaggtaat ttcgcccttc atcagcgcgg cgagtggttg ctgacctcgt ttggccttgc
     2161 tcggaacgtt gccgccgaga tcgagagggc gcctgtgcac attgtcgtcg tcgagtgtcc
     2221 tgtcaagcgc gtcggtgagc gtgtcgagga ttggctgtga tacgctcaat tgagtaggta
     2281 gttgatcccc accttcacca tctggatgtt gcggttgcta acggtgaaaa cgtcgttggc
     2341 gaggaatgga gatccagccg gaccagtaaa tgtctggctg cccagtccca gataatcgta
     2401 ctcgagcttc gccgaccagt ggttcgcgaa agcccattcg atgcccgcgc cgaccagcca
     2461 accgctgttg
//
LOCUS       DATLJT010001593         1586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3604585,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001593 DATLJT010000000
VERSION     DATLJT010001593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3604585"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VM910_36480"
     CDS             <1..284
                     /locus_tag="VM910_36480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI68039.1"
                     /translation="STGIMLRLGRVYRGMMVNMPPTNAKLKRRAESMVARIAGCSEAK
                     AADALEETQGDIKIAALVVLGHDKAEAERILLRHNGNLRRVLAELNAGR"
     gene            323..1342
                     /locus_tag="VM910_36485"
     CDS             323..1342
                     /locus_tag="VM910_36485"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVI68040.1"
                     /translation="MAEPVSSMAQEICESADVVARILRDRDAVADVARRLDIASAPLG
                     VLCGRGSSGHVGVFLRYLIETRLHLPVSASAPSVITAFRKPLTLRNALFIVISQSGRS
                     PDLVAATRSARDSGARTVAIVNAAESPVADAAEFVIPIEAGAEHSVAATKTVIGSMAA
                     GAGLIAELAGDAALSSALDRLPERLRRALTLDWSAVAGDLAGASAVFVASRGLGLGSA
                     REIALKLAEILRLPALGFSAAELQHGPRAALSSRTPVILMRLMDETAATLDALAHELA
                     ESGIALHLCGGPRGTLPWLGDDDPATDAITMLVPAYRMIERAARAWGFDPDRPPQLSK
                     VTETF"
     gene            complement(1386..>1586)
                     /locus_tag="VM910_36490"
     CDS             complement(1386..>1586)
                     /locus_tag="VM910_36490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68041.1"
                     /translation="CCGFCWVCFTERTQFEPDAFFAGVLRVDFGSYLQAFILSGILCL
                     VAALLVLFVGGETKTSAQPSAA"
BASE COUNT          279 a          529 c          522 g          256 t
ORIGIN      
        1 tatcgaccgg catcatgctg cgcctcggcc gcgtctatcg cggcatgatg gtgaacatgc
       61 cgccgaccaa cgccaagctg aaacggcgtg cggagagcat ggttgcccgg atcgccggat
      121 gcagcgaagc gaaggctgcc gatgcgcttg aagagacgca aggggatatc aagatcgccg
      181 ccctcgtggt gctgggccac gacaaggcgg aagccgagcg cattcttctg cgccacaacg
      241 gcaatctccg ccgcgtgctc gctgagctca acgccggcag gtgacatcgg aaatctcggc
      301 aacgaaggga aagctctgaa ccatggccga gcctgtgtcg tcgatggcgc aagagatctg
      361 cgagagcgcg gatgtcgttg cgcgcatcct ccgcgaccgc gatgccgtgg ccgatgttgc
      421 aagacggctg gatatcgcca gcgcgccgct cggcgtgctg tgcggccgcg gcagctccgg
      481 gcatgtcggt gtgttcctgc gctaccttat cgagacacgg ctgcatctgc cggtatcggc
      541 cagcgctccg tcggtgatca cggcgtttcg caagccgctg acattgcgga atgcattgtt
      601 catcgtgatc tcccaatccg ggcgcagccc cgacctggtg gcggcaacgc ggtccgcccg
      661 ggactcgggg gcgcgcacgg tcgccatcgt caatgcggcc gaatcgccgg tcgccgacgc
      721 cgccgaattc gtcattccga tcgaagccgg agccgagcat tcggttgccg cgaccaaaac
      781 cgtgatcgga tcgatggcgg ccggggccgg gctgatcgcg gagctcgccg gtgatgcggc
      841 gctttcctcg gcgctcgacc ggcttccgga gcgtcttcgc cgcgcgctga cgctggactg
      901 gtccgcagtc gcgggcgacc tcgccggcgc atccgccgtg ttcgttgcct cacgcggcct
      961 cgggctcggc tcggcccgcg aaatcgcgct gaagctcgcc gaaatcctgc ggctcccggc
     1021 gcttggcttc agcgccgccg agctgcagca cgggccgcgg gctgcgctgt cttccaggac
     1081 gccggtcatc ctgatgcggt tgatggatga gacagccgcc accctcgatg cgctggcgca
     1141 cgagctcgcg gagagcggga ttgccctgca tctgtgcggc ggtccgcgcg gcacgctccc
     1201 ctggctgggc gacgacgatc ccgcaaccga cgcgatcacc atgctcgttc cagcctatcg
     1261 catgatcgaa cgggccgcgc gcgcctgggg attcgatccg gaccgtccgc cgcagctcag
     1321 caaggtcacc gagaccttct gaggacccgc agtcgaagcc ctgcccaacg tgatcggcat
     1381 cagcgtcatg ccgcagaggg ttgcgccgat gtctttgtct cgccgccgac aaaaaggacc
     1441 aacagcgcgg cgaccaggca caggattccc gacaggatga aagcctgcag atagctgccg
     1501 aaatcgacgc ggagcacgcc ggcgaaaaag gcatcaggct caaattgggt tcgttcggtg
     1561 aaacaaaccc aacaaaaacc acagca
//
LOCUS       DATLJT010001594         9055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_385778,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001594 DATLJT010000000
VERSION     DATLJT010001594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385778"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..506
                     /locus_tag="VM910_36495"
     CDS             165..506
                     /locus_tag="VM910_36495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68042.1"
                     /translation="MKPPESLSEAAKAEFIRIVMAEKADHFRKSDLSLLTQYCESAAL
                     AERAVRELQREDTRAGWLAKWEKANRNLVALSARLRICPQSRQPNNPKRPQPTSYYEQ
                     MRLEDGFDETD"
     gene            493..852
                     /locus_tag="VM910_36500"
     CDS             493..852
                     /locus_tag="VM910_36500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68043.1"
                     /translation="MKRIDRDALERCIATMRNSKEPSDRMQIERALAEDGWQEAADSA
                     AYHCQMNALRLRPWQSPPCWINTRDITSIIARGDDSIAGDYAAAKLLQKMLQASLSRF
                     EPDPVAALKRTNPPRAA"
     gene            complement(856..1539)
                     /locus_tag="VM910_36505"
     CDS             complement(856..1539)
                     /locus_tag="VM910_36505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVI68044.1"
                     /translation="MNQKLSNPATSAVDAFVNQVRTPSLEGAGGGRLIFAIDATASRQ
                     PTWDTACQLQVDMFQEAAAIGGLEVQLVYYRGSGECRASRWVSQPYHLAGLMERIHCR
                     MGQTQIVRILEHARREAERTKVAALVFIGDANEDLLDEVAREAAALGRAGVPAFVFQE
                     GSDEQVEQVFREIAMLTHGAYGRFDTGAAAQLRELLRAVAAFAAGGLKALASRSDTTT
                     QKLLAQLKR"
     gene            complement(1739..3790)
                     /locus_tag="VM910_36510"
     CDS             complement(1739..3790)
                     /locus_tag="VM910_36510"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HVI68045.1"
                     /translation="MTDVLALRQQLRESGYCPIPLYGKEPPIYGKNNKHKGLDGWQTL
                     NDITPEQIELWSKLWPDAQNTGILTQRVPTLDLDILNEEAAEGCENYTHEQYEDRGSF
                     LVRIGKAPKRAILFRTDEPFAKIVINLSAANGNSEKIEFLCDGQQVVVDGIHPDTQQP
                     YDWFGGQPGPISRDDLPYIREQEARVLVDALVDLLVRDFGYTRAAERPKKAGGNGVGA
                     PADWQYLTDNIQAGRELHDSLRDLAAKLITAGMAAGAAVNYLRALLTGSAAPRDKRWQ
                     ERYDDIPRLVRGAEELKEEKEEQARQAAQAAAATQAAQARPPSTLEQTLEVFDRWLIL
                     KDHTPIYAVLGALAANLLPGDPVWLGLVGPPSSAKTEILNSTSLVPNVVQASTLTAPG
                     LLSGVPRRQQTATAKGGLLRQIGSFGIIVLKDFGSILSMRPDLKAELLAALREIYDGA
                     WTRHIGSDGGRTLTWKGKVGLLFGSTGVIDVHYSVFGAMGDRFLLNRLAPIGKGQFEH
                     ALKHVGTGAAQMRKELAEAVANLFAGLGNQPQPMIDADEIYRLDRTLALVVKLRGSVE
                     RDRIKREIEAVHGAEGPARIGLMLERLLAGLSLIGVDRETALEVVETVAMDSVPPLRR
                     KAYECLALAHPRSISTTAVAKGVKLPTNTVRRGLEDLAAYAVAERLSQGQGKADLWRL
                     L"
     gene            complement(3787..4242)
                     /locus_tag="VM910_36515"
     CDS             complement(3787..4242)
                     /locus_tag="VM910_36515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68046.1"
                     /translation="MTDALAPIAEKIKRYVRLLASNRDGEVTAAARALNKTLQNASLD
                     MHALADSIGSANGKKFSEEEARQIYFRGVEDGKRQAEREAGPIFHGVGDDEPSWHEIA
                     VKCAAHPRLMHSDSERKFVHDMVHRTVHGGEPTQKQAAWLRKIYARTQR"
     gene            complement(4311..4907)
                     /locus_tag="VM910_36520"
     CDS             complement(4311..4907)
                     /locus_tag="VM910_36520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68047.1"
                     /translation="MTDDNGDWINDAGTQQREFDTLPADIYRVRAKVKPGGVVDPLCV
                     AKNGYTEHLKIENTVIEGPYKGAKIFDYVTLRYSKGGDDPDVPALTAQQIDNYETAVE
                     IGRVRLRAIVDSAHGLRHKDDSDQAKATRRAIAQDLLRVSGLTYWAHVDIEKGRNGYK
                     DSNRIDRIVTVDDDDWPGKKAQRGLVPRDKDLNDDIPC"
     gene            complement(4897..5127)
                     /locus_tag="VM910_36525"
     CDS             complement(4897..5127)
                     /locus_tag="VM910_36525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68048.1"
                     /translation="MTDHLRFVRDHQLRDLTERLDGALTTLTLCEPVSVPESDDLVAL
                     INRNGIIRIENAITEAVAFLHEMRMKGIHNDR"
     gene            complement(5124..5321)
                     /locus_tag="VM910_36530"
     CDS             complement(5124..5321)
                     /locus_tag="VM910_36530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68049.1"
                     /translation="MSKPRRRTWREAELREAIRVGLPYDRSDYVVYEDTIKRGTKIEI
                     ESVVTNIGHYLDNILKRRGRA"
     gene            complement(5318..5536)
                     /locus_tag="VM910_36535"
     CDS             complement(5318..5536)
                     /locus_tag="VM910_36535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68050.1"
                     /translation="MQSYIQGAAPKTTPAIHRKPHNLKVSKRISELERSLHSLCEYYL
                     QGENDLWTIETMGETAAELMALLKKSAA"
     gene            complement(5680..5898)
                     /locus_tag="VM910_36540"
     CDS             complement(5680..5898)
                     /locus_tag="VM910_36540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68051.1"
                     /translation="MTDTNTAGTTQPKKKKRNKRRVSWRHYAEARGVSVRTLDRWAAA
                     GIIPTPGYIRKRKYIDPETEPRRDADAA"
     gene            complement(6005..6592)
                     /locus_tag="VM910_36545"
     CDS             complement(6005..6592)
                     /locus_tag="VM910_36545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68052.1"
                     /translation="MAKLASKSSAVALISLSKARALFPDNIAWEIAWEKLVHGMAARG
                     VPYSPDDPDLGELWRKLSRGNPDITLPPRFEGDRVEFYIKGVRRRIGPIQIGLKAVVA
                     LQPKDARVPRPDTQEKPRRISPEMWILTEIARLKKEGKISNGISKTELARDFLEPRMR
                     EATKDPSTRLKALRWRTIVNMLANKPGLWPISAIE"
     gene            complement(6657..7643)
                     /locus_tag="VM910_36550"
     CDS             complement(6657..7643)
                     /locus_tag="VM910_36550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI68053.1"
                     /translation="MAQGRDPAAAKQATRRSAAAKTGDTIERWANAFVTRYAKKKTRP
                     NSWRQTEHVLNNIVLPAWKGRTVHDIKRKDVRELLEDLAETKPIMANRTHGVLSKFFK
                     WLMTKLDDADFVSPVIGVERPAEEHVGERVLNDDEIVKLWAACDALGGREGACVKLLL
                     LTGQRRSEISHLKWGEVGNDVLELPAPRMKGKRAHMVPLAAQAAAIIADMPKLVPQVP
                     KPDGCVFGESPVGHFHRIRDRLDEHMGDIPKWVIRDIRRSVASGMARIGIAVPVVEKI
                     LAHRKGTFAGIVGVYQKYGFLPEMTVAMQRWGDHVERLVTARDAKIVKLRKR"
     gene            complement(7700..7867)
                     /locus_tag="VM910_36555"
     CDS             complement(7700..7867)
                     /locus_tag="VM910_36555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68054.1"
                     /translation="MRPLTDIAIHNLRPGSKQYEKPDSAARGLRVVVHPTGRKSFVVR
                     ATPPAGHASSP"
     gene            complement(7938..8498)
                     /locus_tag="VM910_36560"
     CDS             complement(7938..8498)
                     /locus_tag="VM910_36560"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HVI68055.1"
                     /translation="MPSDTKLHVIPSTDLQRFASALFHAAGVAPPMADEWAKSLVWAN
                     LRGVDSHGVLRIPGYIERLNTKAINPAPDMRVEQRSGAVAVLEADRAPGAVAMARAME
                     EAIARAREVHVGWCAARNITHAGAVGYFALQAANTGMAGIVMSASGPMMAYHLGYQDT
                     AGHKRTPDPCFVPMASAYLARCPGLS"
     gene            8624..>9055
                     /locus_tag="VM910_36565"
     CDS             8624..>9055
                     /locus_tag="VM910_36565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HVI68056.1"
                     /translation="MIDETHSPARRSWVASAHQHADFPIQNLPLCVFTPPGGAARGGV
                     AIGDDIFDLAAALELGLFDGVAARAAEAARGATLNPLFALGREARVALRRRLGDILDA
                     DGRERAKVEALRSRLIHRADDCRLELPAAIGDYTDFFAGIHH"
BASE COUNT         1469 a         2848 c         2814 g         1924 t
ORIGIN      
        1 agccgccagc accggaggta tttgtccctg ccttcgccgc gtggccggct gcgctacgcc
       61 aaccggctcc accggccacg ctggcgcaac ggccagcctt aaactaacat tccacctggc
      121 ccactcagtg ggggccaatc accgtcgcct cggttcccca gcgcttgaaa ccgcccgaaa
      181 gcctgtcgga ggcggcgaag gccgaattta tccgcatcgt catggccgaa aaggccgacc
      241 acttccgcaa gtcggatctt tccctcctca cccaatattg cgagagcgcg gcacttgccg
      301 agcgagccgt gcgcgagcta cagcgcgagg acacccgcgc cggctggctg gcgaagtggg
      361 agaaggcgaa ccggaacctc gtggcactct ccgcccggct tcggatctgc cctcagtcaa
      421 ggcaacccaa caatccgaag cgaccacagc cgacatcgta ttacgagcaa atgaggctcg
      481 aggacggctt cgatgaaacg gattgaccgt gatgcgctcg aacgctgcat cgcgaccatg
      541 cgcaacagca aagagccgag cgaccgcatg cagattgagc gcgcgctcgc agaggacgga
      601 tggcaggaag ccgccgatag cgctgcctat cattgccaga tgaacgccct gcgcttgcga
      661 ccgtggcaat cgccgccgtg ctggatcaat actcgcgaca tcacgtccat catcgctcgc
      721 ggcgacgaca gcattgcagg cgattatgcc gccgccaagc tcttgcagaa gatgctccaa
      781 gccagtctct caaggtttga gccggatccg gtcgccgcgc tcaaacggac gaatccgcct
      841 cgcgcggcct aacccctatc gcttcaactg cgcgagcaac ttctgtgtcg tggtatcact
      901 gcgactggcc agcgccttca atccaccggc agcaaaggcc gcgaccgcac gcagaagctc
      961 gcgtagctga gccgccgcgc cggtatcgaa gcggccataa gccccatgcg tgagcatggc
     1021 gatctcgcgg aagacctgct cgacctgctc gtcgctgcct tcctgaaaga caaaggccgg
     1081 cacccctgca cgccccaagg cagccgcctc gcgtgccacc tcgtcgagca agtcctcgtt
     1141 ggcgtcgccg atgaatacca aggcagcaac cttcgtgcgc tcagcctcgc gccgggcatg
     1201 ctctaggata cggacgatct gcgtctgtcc catgcggcag tgaatccgct ccatcaaccc
     1261 cgccaagtga tagggctgcg acacccaacg tgaggctcgg cattcgcctg acccgcgata
     1321 atagaccaac tgcacctcaa gcccgccgat tgccgccgcc tcctgaaaca tgtccacctg
     1381 tagctggcag gcggtgtccc aggtcggctg tcggctcgcg gtcgcgtcaa tggcgaagat
     1441 aagccttcca ccccccgccc cttcgagcga cggtgtccgc acctgattaa caaaggcgtc
     1501 gacggcagag gtcgcgggat tgctgagctt ctgattcatg tgatgtgctg ccccctttca
     1561 cggcgccgtg gagaacagta ggtggcgtga aagaaatgcc caattttgtt gggctttttg
     1621 cacccccaag agggtgtacc cgcgtttatt ctctctctgg agaaggagaa gttataagta
     1681 acttctcctg tgcactctcc agcgattgtg tataacgcgg atgcccccct cgtcaggatc
     1741 acagaagtct ccataaatcg gcctttccct gcccctgaga taaccgttct gctaccgcgt
     1801 atgccgcgag gtcttcgagc ccgcgacgga cggtgttggt cggcagcttg acgcccttgg
     1861 cgacggcggt ggtgctgatg cttcttggat gggccagcgc caagcattcg tacgccttgc
     1921 ggcgcagcgg cggcacggaa tccatggcga cggtttctac cacttcgagc gccgtttccc
     1981 ggtcgacgcc gatgagggaa agaccggcga gcagccgttc cagcatgagt ccgatgcgcg
     2041 ccggcccttc ggcgccgtgg acggcctcga tttcgcgctt gatgcggtcg cgctcgacgc
     2101 tgccgcgtag cttgaccacc aatgcgagcg tccggtccag ccggtagatt tcgtctgcat
     2161 cgatcatcgg ttgcggctgg ttgcctaggc cggcaaacag attggcaacg gcctcggcta
     2221 gctccttgcg catttgggcg gcgcctgtgc cgacatgttt gagagcgtgc tcgaattggc
     2281 ccttgccgat gggggccaag cggttgagca agaaacggtc gcccatggcg ccgaagacgc
     2341 tatagtgaac gtcgatgacg ccggtcgagc caaacaagag cccgacctta cccttccaag
     2401 tgagcgttcg gccgccatcg ctgccgatgt ggcgagtcca agcgccgtcg taaatctcgc
     2461 gtaaggctgc cagcagttcg gctttgagat ccggtcgcat gcttaggatc gagccgaaat
     2521 ccttgagtac gatgattccg aagctaccga tctgtcgtag caggccgcct ttggcggtcg
     2581 ccgtttgttg gcgccttggc acgcccgaga gcaatccggg cgctgtcagg gtcgaggctt
     2641 gtacgacgtt cggtaccagt gatgtggaat tgaggatttc ggtcttggcg ctcgacggtg
     2701 gcccgaccaa cccaagccaa accggatcac ctggtaggag attggcggca agcgcgccga
     2761 gcacggcata gatcggcgtg tgatccttga gaatgagcca gcggtcgaac acttcgagcg
     2821 tttgctcaag cgttgacggc ggacgcgcct gtgcggcctg tgttgccgct gctgcttgtg
     2881 ctgcctgtct ggcttgttct tccttttcct ccttaagctc ttcggcgccc cggaccaggc
     2941 gcgggatgtc gtcgtagcgc tcttgccagc gcttgtcgcg cggggcggct gagccggtca
     3001 gcagcgcgcg cagatagttg acggcagcgc ctgctgccat gccggcggtg atgagcttgg
     3061 cggcgaggtc gcgcagcgag tcatgtagct cgcgaccggc ttggatgttg tcggtgagat
     3121 attgccaatc ggcgggggcg ccgacgccgt tgccgccggc tttcttcggc cgctcagcgg
     3181 cgcgggtata gccgaagtcg cgcaccagta agtcgactag ggcgtcgacg agcacgcgcg
     3241 cctcctgttc gcggatgtag ggcagatcgt cgcggctgat gggtccgggt tgcccgccaa
     3301 accagtcgta gggctgttgt gtgtcggggt ggattccgtc gacgacgacc tgctggccat
     3361 cacataggaa ttcgatcttt tcgctgttgc cgttggcggc actcaggttg atgacgattt
     3421 tggcgaatgg ctcgtcggtt cgaaagagaa tcgcgcgttt tggcgctttg ccgatgcgca
     3481 cgagaaagga tccgcgatct tcgtattgct catgcgtgta gttttcacac ccttctgcgg
     3541 cctcttcgtt gaggatatcg aggtcaagcg tcggcactcg ctgcgtgagg atgccggtgt
     3601 tctgtgcgtc cggccaaagc ttcgaccata gctcgatttg ttcgggggtg atgtcgttga
     3661 gcgtctgcca gccgtcgagc cccttgtgct tgttgttctt gccgtagatc ggcggttcct
     3721 tgccgtagag cggaatcggg cagtagccgc tttcgcgtag ctgctggcgt agagcgagga
     3781 catcggtcat cgttgtgtcc tcgcgtagat ttttcgcagc caggctgctt gcttctgcgt
     3841 cggctcgccg ccgtggacgg ttctgtgcac catgtcgtgt acgaactttc gctcgctatc
     3901 gctgtgcatc agccgcggat gtgctgcgca cttgaccgcg atctcgtgcc agcttggctc
     3961 gtcgtcccct acgccgtgaa atatcggacc agcctcgcgc tcggcttgac gcttgccgtc
     4021 ttcgacgccg cggaagtaaa tttggcgtgc ttcttcttcg gaaaatttct tgccgttggc
     4081 ggatccgatg ctgtcggcga gtgcgtgcat gtcgagactg gcgttctgta gggttttatt
     4141 gagcgcacgc gctgcggctg tgacctcgcc gtcgcggttg ctggctaaaa gccggacgta
     4201 ccgcttaatc ttctcggcaa tcggcgcgag cgcatcggtc atttgctcgt gtcgtcacgg
     4261 aagcgagggc accaacttgg tgggcggggc tctcgcttcc gtcctccttc tcagcaagga
     4321 atgtcgtcgt tgaggtcttt gtcgcgcggg actaggccgc gttgtgcttt cttgcccggc
     4381 caatcgtcat catcgaccgt gacaatgcgg tcgatgcggt tgctgtcttt atagccgttg
     4441 cggccttttt cgatgtcgac gtgtgcccaa taagttagac cgctgacgcg caaaaggtcc
     4501 tgtgcaatcg cgcgtcgggt cgcttttgcc tgatcgctgt catccttgtg acgaagccca
     4561 tgggcgctgt cgacgatggc gcgcaacctc acgcgtccga tctctacggc ggtttcatag
     4621 ttgtcgatct gctgtgccgt gagtgctggc acatccggat cgtcaccgcc cttgctgtat
     4681 cggagggtga cgtaatcaaa gatcttcgca cctttgtaag gtccctcgat gacggtgttc
     4741 tcaatcttga ggtgctcggt gtagccgttc ttggccacgc atagcggatc gacgacaccg
     4801 cccggcttta ccttcgcgcg cacgcggtag atgtccgccg ggagcgtgtc gaattccctt
     4861 tgttgtgtcc ccgcgtcgtt gatccaatcg ccgttatcat ctgtcattgt ggattccttt
     4921 cattcgcatt tcgtgcaaga aggcgacggc ttccgtgatc gcgttttcga tgcggatgat
     4981 gccgttccgg ttgatgagag cgacgagatc gtcgctctcg gggacgctga cgggctcgca
     5041 caacgtcagc gtggtgagcg ctccgtcgag tcgctcagtc agatcgcgca gttggtggtc
     5101 gcggacgaag cgaaggtggt cggtcatgcc cgcccccgtc gcttgaggat gttgtcgagg
     5161 tagtgaccga tgttggtgac gacgctctcg atctcgatct tggtgccgcg cttgatcgtg
     5221 tcctcgtaca cgacgtagtc gctgcggtcg tacggtaagc caacgcggat ggcctcccta
     5281 agctcggctt cgcgccaagt ccttcgtcgg ggcttgctca tgccgcgctc tttttcagca
     5341 gcgccatgag ttccgccgcg gtctctccca tcgtttcgat ggtccagaga tcgttttctc
     5401 cttgcaggta gtactcgcag agcgagtgca aggatctttc caactcgctg atccgtttgc
     5461 tgaccttcaa gttgtggggc ttgcggtgga tcgcgggggt tgtcttgggc gctgcgccct
     5521 gtatatatga ctgcatagac gtgcctttcc tcggctctgc aaagccggtg ttggatttca
     5581 gaggcggcgg gccttttcag cccgccgctt tcgcgttggt taccccactg tgtggaccgc
     5641 cttggccgcc gggggcggcg cagccgagac ggacccggct caggccgcgt cagcgtcgcg
     5701 tcgcggctcg gtctcgggat cgatgtattt tcgctttcgg atgtacccgg gcgtcggaat
     5761 gatgcctgcc gccgcccacc tatcgagcgt ccggacagaa acgccgcgag cctcggcata
     5821 gtgccgccat gacacgcgcc gcttgttgcg cttcttcttc ttcggctgtg tcgtgcccgc
     5881 tgtgttggtg tctgtcatgt ccgccctcgt catcactttt gcgatgaggg gcagcgaaca
     5941 ccgccacgac gcacattgcg agactccaaa atgtgcgttt gtgcgtgcgt ttgtgcgtcc
     6001 ggggttattc gatggctgag atcggccaga gcccgggctt gtttgcaagc atattcacga
     6061 tggtgcgcca gcggagcgct ttgagccggg tcgagggatc cttggtggcc tcgcgcattc
     6121 tgggttcgag gaaatccctc gcgagttctg tcttactgat gccgttggaa atcttgcctt
     6181 cttttttcag ccgcgcgatc tccgttagaa tccacatttc cggtgaaatg cggcggggct
     6241 tttcttgtgt atcgggccgc ggcacgcgcg cgtccttggg ctgcaacgcg accacagcct
     6301 taagcccaat ctggatcgga ccgatcctgc ggcgaacgcc cttgatgtaa aattccaccc
     6361 gatccccttc gaaacgcggc gggagggtta tgtccgggtt gccccggctc agtttcctcc
     6421 aaagctcgcc gagatcggga tcgtcgggcg aatagggcac gcctctcgct gccatcccgt
     6481 gcactagctt ttcccacgca atttcccacg caatattgtc ggggaacaac gcgcgggcct
     6541 tggacagcga aatcagggcg actgcgctag acttactcgc aagcttggcc aatggggccc
     6601 actcccatgg tcgggcgagg gctggcgagt gttgagcgca ccccgctgcc cgcttgttaa
     6661 cgttttcgga gcttcacgat tttagcgtcg cgggcggtga ccagccgctc gacgtgatcg
     6721 ccccaccgct gcatggcgac cgtcatctcg ggcaggaatc cgtacttctg gtagacgccg
     6781 acgatgcccg cgaaggtgcc cttgcggtgc gcgaggatct tctcgaccac cggcaccgcg
     6841 atgccgatcc gcgccatgcc ggacgcgacc gagcgccgga tgtcgcgtat gacccacttc
     6901 gggatgtcgc ccatgtgctc gtcgagccgg tccctgattc tatggaagtg tccgaccggc
     6961 gattcaccga agacgcaacc gtccggctta ggcacctgcg gcacgagctt cggcatgtcg
     7021 gcgatgatgg cggcggcctg cgccgccagc ggcaccatgt gcgcgcgctt gcccttcatg
     7081 cgcggggctg gcaattccag cacgtcgttg ccgacctcgc cccacttgag gtgcgaaatc
     7141 tcgctccgtc gttggccggt aagtagcagt agcttgacgc aagcgccttc ccggccgccg
     7201 agcgcatcgc aggccgccca tagcttgacg atctcgtcgt cgttgagcac gcgctcgccc
     7261 acgtgctctt cagccggacg ctcgacgccg atgacggggg aaacgaaatc agcgtcgtcg
     7321 agcttggtca tcaaccactt gaagaacttg ctcagcaccc cgtgcgtccg attcgccatg
     7381 atgggcttgg tctcggcgag gtcttccagc aactcgcgca catccttacg cttgatgtcg
     7441 tggacagtgc ggcccttcca cgcgggcagc acgatgttgt tgaggacgtg ctcggtctgc
     7501 cgccagctat tcggccgcgt cttcttcttg gcgtagcgcg tgacgaacgc gttcgcccac
     7561 cgctcgatgg tgtcgccggt cttggcggcg gcgctgcgcc tggtcgcctg cttggcggcg
     7621 gccgggtcgc ggccctgcgc cacctccaag agcgcatcgc cagcgagctt gcgggcggcg
     7681 gcgagcgtga tgccgggggt caaggtgagc ttgcgtgtcc ggccggtggc gttgcgcgca
     7741 cgacgaagct cttgcggccg gtcggatgca cgacgacgcg caagccgcga gcagcgctgt
     7801 ccggcttctc gtattgctta ctgcctggtc tgaggttgtg gatggcgatg tcggtgagcg
     7861 gtcgcatggg ttgatgatgt ccttccgagg taacgcatag gtaacaaaat tggctgtctg
     7921 cgcgtgtctt ggtctgtcta ggacaagccc ggacaacgtg ctagatatgc cgatgccatg
     7981 ggcacaaagc aagggtccgg tgtccgtttg tgtccggccg tgtcctggta cccgagatgg
     8041 taggccatca tcggtcccga cgccgacatc acgatgccgg ccatccccgt gttcgcggcc
     8101 tgcagcgcga agtagccgac ggcgcccgcg tgggtgatgt tgcgcgcggc gcaccagccg
     8161 acatggactt cgcgcgcccg cgcgatcgcc tcttccatag cccttgccat ggcaaccgct
     8221 cccggcgcgc gatccgcctc caaaaccgcg accgcgcctg agcgctgttc cacccgcatg
     8281 tcgggggcgg ggttgatggc cttggtgttc agccgttcga tgtagcccgg gatccgcaac
     8341 accccatgcg aatcaacgcc ccgcagattc gcccacacca acgacttcgc ccattcatcc
     8401 gccatgggcg gcgcaacacc cgccgcgtgg aacagcgcgc ttgcgaagcg ctggagatcg
     8461 gtgcttggaa tcacgtgcaa tttggtgtcc gatggcatct cggtaacctg caggaattct
     8521 cgcgaaggac tcaggacata agtcgcggac gatctctata cgaaccggtt catcaagtaa
     8581 caatcccgac tgccgctctg aggcagcgcg aaaagatcta gccatgatcg acgagaccca
     8641 ttcacccgcg cggcgcagct gggtcgcctc ggcccatcag cacgccgact ttccgatcca
     8701 gaacctgccg ctctgcgtct tcaccccgcc gggcggcgct gcccgcggcg gcgtcgcgat
     8761 cggggacgac atcttcgatc tcgccgcggc gctcgagctc ggcttgttcg atggggtagc
     8821 ggcacgcgcc gccgaggccg ctcgcggcgc gacgctcaac ccgctgttcg cgctcggccg
     8881 cgaggcacgc gtcgcgctcc gccgccgcct cggcgacatt ctcgatgccg atggccgcga
     8941 gcgcgccaag gtcgaagcgc tgcgatcgcg cctcattcac cgcgccgacg attgccgcct
     9001 cgagcttccg gccgcgatcg gcgactacac cgacttcttc gccggcattc accac
//
LOCUS       DATLJT010001595         1540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_843317,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001595 DATLJT010000000
VERSION     DATLJT010001595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843317"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VM910_36570"
     CDS             complement(<1..198)
                     /locus_tag="VM910_36570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68057.1"
                     /translation="MPRFAFAQAYPTRPVRIIVGFPAGLGPDITARLIAQLLSERFGQ
                     SFIVDNRPGAGSNIGTEIVVRA"
     gene            complement(284..1276)
                     /locus_tag="VM910_36575"
     CDS             complement(284..1276)
                     /locus_tag="VM910_36575"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68058.1"
                     /translation="MTVSIGRRELLAALGGAAAAWSFAARAQQPGKTPRVGYIRAGTP
                     HNDPFREEFVRGMHDLGYVEGRNIAYEFRHYGDDVESIPSLIGDLLRANVDIIVAGGT
                     VAVRAAQTATQSIPIVIGAAADPLGVGLIAGLARPGGNTTGFSVLSTELTVKRLELLR
                     EVMPQAARIAILQKPGNPAHPMFIREIEPTAGLLGFTFRNFEARGSEDFEPSFASMRA
                     WLADAIFVLDDPTFIASRAAMAEAAARHRLPLICGFREMTEAGCLFSYSASLKDIWYR
                     SATYVDKILKGAKPADLPVQQGTKFEFVINLKAARALGLDLSPILLARADEVIE"
BASE COUNT          300 a          474 c          463 g          303 t
ORIGIN      
        1 tgcacgcacg acgatttcag tgccgatatt gctgccggcg cctggtcgat tgtcgacaat
       61 gaaagattgg ccgaatcttt ccgatagcag ttgagctatt aagcgcgcgg tgatgtcggg
      121 tccgagccca gcagggaagc caacaatgat gcgtaccggc cgcgtcggat aggcttgcgc
      181 gaaagcaaag cgcggcatgg cctggagcgc ggcggcgccc gctgccagat gcagaaaatt
      241 tcggcgggga agtttcattt ttgaaccccc cttttgcttc acatcactcg atcacctcgt
      301 cggcgcgggc gagcaagatc ggcgagaggt cgagacccag cgctcttgcg gccttgaggt
      361 tgatcacgaa ctcgaacttg gtcccctgct ggaccggcaa atcggctggt tttgcacctt
      421 tgaggatttt gtcgacgtag gtggcggatc gataccagat gtctttgaga ctagccgaat
      481 acgagaacag gcaacccgcc tcggtcatct cccggaatcc gcagatcagc ggcagacggt
      541 ggcgcgccgc cgcctccgcc attgctgctc gagaggcgat gaaagtagga tcatccagta
      601 cgaagatggc gtcggccagc cacgctcgca tcgacgcgaa actcggctca aagtcttccg
      661 accctcgtgc ttcgaaattg cgaaaggtga atccgagtaa cccggcggtc ggttcgatct
      721 ctctgatgaa catcggatgc gccggattgc ccggcttctg gagaattgca atccgggccg
      781 cttgcggcat gacctccctg agcagttcta gccgcttcac ggtcagttcc gtggacagga
      841 cggagaaccc cgtcgtgttg ccgcccggac gcgccagacc tgcgatcaag ccgacgccca
      901 acggatcggc cgccgctccg atgacgatgg ggatgctctg tgttgcggtc tgcgcagcgc
      961 ggaccgcaac ggtaccgccg gcaacgatga tatcgacatt cgccctcagc aaatcgccga
     1021 ttagcgaggg tatggattcg acgtcatcgc cataatggcg aaactcatag gcaatgttgc
     1081 gcccttcgac ataaccgagg tcgtgcatcc cgcgcacgaa ttcctcacgg aagggatcgt
     1141 tgtgcggggt tcccgcccgg atgtacccga cacgcggcgt cttgcccggc tgctgcgcgc
     1201 gcgcagcgaa cgaccaagca gcggcagccc cgccgagtgc ggccagcaat tcccgccgtc
     1261 cgatgctgac tgtcatacgc ccctcccgtg cgaggtgcgt aaagggcacg ataccacgcc
     1321 acgagcgtgc agtcttcacg ttagcatgct ggaattcgtg cgtcgcgatt gctgacgcgc
     1381 caaatccccc tctgcttcgc gcgtcaccgc tggcgcattt gagggcctat tttcgacagc
     1441 actttctcgt gtgtccattt gcacggcgtg atcgtgtcct gcggatagat acacacatat
     1501 ggcccgcggg ttgcgtagat acgaacccgg cgccctgcac
//
LOCUS       DATLJT010001596         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2686101,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001596 DATLJT010000000
VERSION     DATLJT010001596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686101"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /gene="katG"
                     /locus_tag="VM910_36580"
     CDS             <1..1722
                     /gene="katG"
                     /locus_tag="VM910_36580"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HVI68059.1"
                     /translation="LAGNVALDSMGFKTFGFGGGRADVWEPEELYWGPEGTWLGDERY
                     SGERQLHEPFGAVQMGLIYVNPEGPNGKPDPVAAAKDIRETFFRMAMNDEETVALIAG
                     GHTFGKTHGAGDPSLMGPAPEGALIEDQGLGWKSKYGTGFGADAITGGPEVTWSQTPT
                     KWSNHFFENLFNNEWELTKSPAGAYQWKAKGTAATIPDAYDKSKKHVPTMLTTDLSLR
                     LDPAYEKISRRFYEHPDQFADAFARAWFKLTHRDMGPIHRYLGPLVPKEILIWQDPIP
                     AVDHPLVDDKDVATLKAKILASGLSVSQLVSTAWASASTFRGSDKRGGANGARIRLAP
                     QKDWEVNQPAELAKVLQKLAAIQKEFNASASGGKKISLADLIVLAGGAAVEKAAKDAG
                     SDVKVPFTPGRMDASQEQTDVESFAPLEPRADGFRNYIGGKRQFMLPEEALVDRAQLL
                     TLTGPELTVLIGGLRVLGANAGQSKHGVFTKRPGTLTNDFFVNLLDMNTQWQPAGSEG
                     VYEGRDRKTNELKWTGTRVDLIFGSHSQLRAFGEVYACSDSKEKFVKDFVAAWAKVMN
                     LDRFDLA"
     gene            complement(1834..>2597)
                     /locus_tag="VM910_36585"
     CDS             complement(1834..>2597)
                     /locus_tag="VM910_36585"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVI68060.1"
                     /translation="DQRMRDFALTVADINALDPAPDVIVHTGDIVHNGRQDEYAQAVA
                     TLAKARAPVYVLGGNRDNRANLRAAFSASGYLASDSDFIDYAIDDYPVRLIALDTLSS
                     GSNKGDFCAQRVRRLVELIDAETTKPIAVFTHHPPFEVLVGPDRLHFETTDMVSRLRQ
                     ALQHSGRVVAVFSGHVHRAAAGYVGSIPATVVQCIATTLRKGEYPPHMKIRPVYQLHR
                     FDPVWGFASETRIVDARPVAPGRCDSALPRRKRGR"
BASE COUNT          510 a          817 c          830 g          440 t
ORIGIN      
        1 ctcgccggca acgtcgcgct cgactcgatg ggcttcaaga cgttcggttt cggcggcggt
       61 cgcgccgatg tctgggagcc cgaagagctc tactggggtc ccgaggggac ctggctgggg
      121 gacgaacgct acagcggcga acgccagctg cacgaaccgt tcggcgcggt gcagatgggc
      181 ctcatctacg tcaatccgga aggaccgaac ggcaagccgg atccggtcgc cgcggccaag
      241 gacatccggg aaacgttctt ccgcatggcg atgaacgacg aggagacggt tgcgctcatt
      301 gccggcggcc acaccttcgg caaaacccac ggcgccggcg atccgtcact gatgggcccg
      361 gcgccggaag gcgccctcat cgaggatcag ggcctcggct ggaagagcaa gtatggcacg
      421 ggcttcggcg ccgatgccat taccggcggg ccggaggtca cctggtcgca gacgcccacg
      481 aagtggagca atcacttctt cgagaacctc ttcaacaatg aatgggagct gacgaagagc
      541 ccggccggcg cctatcagtg gaaggcgaaa ggcaccgctg ccacgatccc ggacgcctac
      601 gacaagtcga agaagcacgt gccgaccatg ctgaccacag acctctcgct gcggttggac
      661 ccggcctacg aaaagatctc gaggcgcttc tacgagcatc cagatcagtt cgcggacgcg
      721 tttgcccgcg cctggttcaa gctcacgcac cgcgacatgg gtccgatcca tcgctacctc
      781 ggcccgctcg tgccgaagga aatactgatc tggcaggacc cgatcccggc cgtcgatcat
      841 ccgctggtcg acgacaagga cgtcgccacg ctcaaggcga aaatcctcgc ctccggcctg
      901 tcggtgtcgc agctcgtgtc gacggcctgg gcgtcggcgt cgacgttccg cggctccgac
      961 aagcgcggcg gcgccaatgg cgcgcgcatt cgcctcgcgc cgcagaagga ctgggaggtg
     1021 aaccagccgg ctgaactcgc gaaggtgctt cagaaactcg cagcgatcca gaaggagttc
     1081 aacgcgtcgg cctccggcgg caagaagatt tcgcttgccg acctgatcgt actcgccggc
     1141 ggcgccgcgg tcgaaaaggc cgccaaggac gccggcagcg acgtgaaggt gcccttcacg
     1201 ccggggcgca tggatgcgtc acaggagcag accgacgtcg agtccttcgc gccgctcgag
     1261 ccgcgtgccg acggcttccg caactatatt ggcggcaagc ggcagttcat gttgcccgag
     1321 gaggcgttag tggatcgggc gcaattgctg acgctaaccg ggccggagct gaccgtgctc
     1381 atcggcgggt tgcgcgtgct cggcgcaaat gccgggcaat ccaagcacgg cgtcttcacc
     1441 aagcggcccg gcacgctaac gaacgacttc ttcgtcaacc tgctcgacat gaacacacaa
     1501 tggcagccgg ccggctctga aggcgtttac gaggggcgcg accgcaagac gaacgagctc
     1561 aagtggaccg gcacgcgcgt cgatctgatc ttcggttcgc attcgcagct gcgcgccttc
     1621 ggcgaagtct acgcttgctc ggactcgaag gagaagttcg tgaaggactt cgtggcggcg
     1681 tgggccaagg tgatgaatct cgaccgcttc gacctcgcct gatctcggcg gaaaggacgg
     1741 tgctggtcca gatcatcaag gggccgacgg gatagattcc ggttgctccg cggacacggc
     1801 gcgcgagggc agcacgtcgc ccgccatgca acgttagcgg ccccgctttc gccggggcag
     1861 tgcggaatcg catcttcctg gcgccactgg tcgcgcgtcg acgatccgtg tttcgctggc
     1921 aaagccccaa accggatcaa atcggtggag ctgatagacc ggacgtatct tcatgtgagg
     1981 cggatactcg cctttgcgca gtgtcgtcgc gatgcactgc accactgtcg ccggaatgct
     2041 gccgacataa cctgcagcag cacgatgaac gtggccgctg aaaacggcga cgacccgccc
     2101 ggagtgctgc aaagcctggc ggagcctcga caccatatcc gttgtttcga agtggagccg
     2161 atccggcccc actaaaacct caaaaggcgg atggtgcgtg aaaaccgcaa taggcttggt
     2221 ggtctcggcg tcgatcagct caaccaaacg tctgacccgc tgggcgcaaa agtcaccctt
     2281 gttgctgccc gaactcaggg tatccagcgc aatcagtctt accgggtaat cgtcaatcgc
     2341 atagtcgatg aagtcggaat cggacgcgag atatccggag gccgaaaagg ccgcgcgtaa
     2401 attcgccctg ttatccctat tgccgcccag gacatatacc ggcgcacgcg ctttcgcgag
     2461 cgtggctact gcctgagcgt attcgtcttg ccgtccgtta tgaacgatat ccccggtgtg
     2521 gacgatcacg tcgggggctg gatcgagcgc gtttatgtcc gcaaccgtaa gtgcgaaatc
     2581 gcgcattctc tggtcgg
//
LOCUS       DATLJT010001597         6113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_843427,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001597 DATLJT010000000
VERSION     DATLJT010001597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843427"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..1776
                     /locus_tag="VM910_36590"
     CDS             520..1776
                     /locus_tag="VM910_36590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HVI68061.1"
                     /translation="MPRRIWRRGGDLRRRLYEILEHGTIGDRTGLIVGRLIVVLIVTN
                     LVTMTLDSVPALQARYGPLFIAIELLSLVVFTIEYALRVWVAAERAPHRHSSERKARW
                     EFISSPLGVIDLLAVLPFWFAFVLPGDLRVLLVFRMVRFLKLARYSPAMRSLLDALYN
                     ERRALFGCFVILLGATLVAASVMHVIEGHAQPDKFGTIPDAMWWAIVTLGTIGYGDVV
                     PVTVLGRIVASVTIFVGLIMIALPIGIVATAFADEIHRRDFVVTWGMVARVPLFAELQ
                     ATDIADIMRLLRAQQVEAGDIIAKRGEPAHSMYFVAAGEVEIELKHERVRLGAGHFFG
                     EVAALRRARRSATVTALTRTSLLVLDAQDLHVLMEREPRIAEHIREVARTRLGGDVVT
                     RKGDLVVEELEEAEAIQRPAGSHGRS"
     gene            complement(1785..2645)
                     /locus_tag="VM910_36595"
     CDS             complement(1785..2645)
                     /locus_tag="VM910_36595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVI68062.1"
                     /translation="MATVNTTRSERSAGSEQATSFSTMMVHVDVEHDSKRRVELAIGL
                     ADRFQAALIGVAGCALWPAFTAGDVGLTKSSEYDVQKLMARFEQRGKTFRAQGRSLRH
                     IEWRSALESPGELLLREARAADLLVVGRRHSENDADPGVIVLRAGRPVLLVPDTVAAL
                     PLRHAVVAWKDTRESRRAVRDALPFLQQAKEVVLVEVGEDESQWQAKKNLADVAAYLV
                     RHRVIVAAEVWRRPRGPVAAELLHVVAEEKADLIVAGGYGHSRLGEWIFGGVTRELLT
                     ASPVCCMLSH"
     gene            complement(2661..3152)
                     /locus_tag="VM910_36600"
     CDS             complement(2661..3152)
                     /locus_tag="VM910_36600"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVI68063.1"
                     /translation="MSRVLVAIGVCVMTGGAPVAAQQSPSPSVDKDAIVLIPGVLTGP
                     GTMGPRGYKRLCTPRSVGLYEWQVRWIERVVRPTEAQRAALNDLQSASSKAIATMATA
                     CKSDVPATTKAQLQMMDNRLETMSQAVKSLRPTFDAFYDSLDSKQQQRLDVFGPRHRS
                     WRW"
     gene            complement(3352..3687)
                     /locus_tag="VM910_36605"
     CDS             complement(3352..3687)
                     /locus_tag="VM910_36605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI68064.1"
                     /translation="MRRQILHCLVTMMAMSVIAPVLAQGTGTPAGRGEFLLTTHCARC
                     HAVGRTGASPHPEAPPFRMLSRKYKIEGLAEALAEGLSTGHPDMPEFVFAAEDVGAII
                     DYLQSIQQQ"
     gene            complement(3690..4139)
                     /locus_tag="VM910_36610"
     CDS             complement(3690..4139)
                     /locus_tag="VM910_36610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68065.1"
                     /translation="MLLVATPCGHAQARAQAEAMKSQGEASNSGVVVIVVSDDLAVRN
                     SLKFWLEIEGLTVRGYVSATDLLDAGDLARCDCYLVDQKMPAMSGLDLIAQLRDRHLT
                     APAILIASHPSRLLREQAEKADIPIVEKPLLGNGLLDKIRDVVRGHD"
     gene            complement(4265..4633)
                     /locus_tag="VM910_36615"
     CDS             complement(4265..4633)
                     /locus_tag="VM910_36615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68066.1"
                     /translation="MSPQTSLRSDLLSSTGRALRYLVSGCSVLLAIVSIVIGIEVSVA
                     TASSGAAPSTEIINRTQKGDRLLLVPALHRNPVNRPPEVSLPRMHILAPDQDLADGCE
                     SSVSPLARSPLAQIAGRCLS"
     gene            complement(4744..5373)
                     /gene="fixJ"
                     /locus_tag="VM910_36620"
     CDS             complement(4744..5373)
                     /gene="fixJ"
                     /locus_tag="VM910_36620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HVI68067.1"
                     /translation="MPPDKSIVHVIDDDEAVRQALAFQLGSAGIEVRTHESAVAFLQV
                     APTVRTGCIITDVRMPELSGIDLLRRLRELKHPVPVIVITAHGDIPLAVEAMRLGAVD
                     FLEKPFEDEVLLASVRSALDQGDRDQKRQAERSNIEARLAALSNRERDVLKGLVAGLA
                     NKQIAYDLGISPRTIETYRANLMIKMQAASLSDLVRMALIAGVLDSNES"
     gene            complement(5363..>6113)
                     /locus_tag="VM910_36625"
     CDS             complement(5363..>6113)
                     /locus_tag="VM910_36625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68068.1"
                     /translation="EARLQELQSELVHVSRLTAMGQMASALAHELNQPLLAITGYMKG
                     SRRMLESGGEDRSGLLRDALDKAGDQALRAGQIIRRLREFVARGDSERRVESVKKLIE
                     EASALALVGTKDQGVRVRFQFDPAVDLVLVDKVQIQQVLLNLLRNAVEAMEASQRREL
                     VISTAAGDGEMVAVSVADTGSGIAQDLMSQLFQPFVTNKRHGMGVGLSICRTIVEAHG
                     GQITVEPNPDGGTIFRFTVRAASEEDFGDAA"
BASE COUNT         1116 a         1937 c         1876 g         1184 t
ORIGIN      
        1 gcgtgcgtat ccccgtgtgc catcgcggct ggaggcgagg cgggactttg gggtggagct
       61 ggcgcctgcg ccaccatggg ctgcgctgaa tgtgagtgct gttgcgccca ggcttgatgg
      121 gtgcatccca cgagagttgc cgcgaacaac agcgcgttcc acatccgcat cggattcatc
      181 ctttttgacc gctgcttcca ttgtcggcaa cggaacatca ccatccattt gggaatgatc
      241 cgccaatcag tcggaaggga acctgacata gatcaaatcg aggcgccgat aagtccgctg
      301 ccgccagggg tcggtcgggc ggaagcgcgg cctctgtgga gtggtcaatc aagctggtgg
      361 gcagccgcgt ggagccgcgg ctggcggctc gcatacgcgc atacgcatcg atttgccgcg
      421 tttgacaaat atcaaaggtc gcgatcgcca tatctgacaa cctgccacga gggtgagtcc
      481 cacaccttca aggtcgtgtc ggtcggatag agctcagcta tgccgcgacg tatctggcgc
      541 aggggcggag atctccggcg ccgcctgtac gagatcctgg agcacggcac gatcggagat
      601 cggacgggtc tcatcgtcgg ccggctcatc gtcgttctga tcgtcacgaa tctcgtcacc
      661 atgacgctcg actcggtgcc tgcgttgcag gcgcgatacg ggccgctatt catcgcgatc
      721 gagctgttat cgctcgtcgt tttcaccatc gagtacgcac tgcgggtttg ggtggcggcc
      781 gagcgcgcac cgcaccggca ttcgagcgaa cgcaaggccc gctgggagtt catttcgagc
      841 ccgctcggcg tgatcgatct tctcgcggtc ctgccgtttt ggttcgcgtt cgtcttgccg
      901 ggcgatctgc gggtcctgct ggtgtttcga atggtgcgct ttctcaagtt ggcgcgatac
      961 tcgccggcca tgcgctcgct gttggatgct ctctacaacg aacgccgcgc gttgttcggc
     1021 tgcttcgtca ttttactggg tgcgacgctg gtcgctgcca gcgtcatgca tgtgatcgag
     1081 ggtcatgcac agcccgacaa attcggcacc attcccgacg cgatgtggtg ggccatcgtc
     1141 accctcggca cgatcggtta cggcgatgtc gttccggtga ccgtgctggg gcgcatcgtc
     1201 gcatcggtta cgatcttcgt cggcttgatc atgatcgccc tgccgatcgg catcgtcgct
     1261 accgccttcg ccgacgaaat ccaccgtcgc gactttgtcg tcacttgggg catggtggcg
     1321 cgggtgccct tgttcgccga gcttcaagcc accgatatcg ccgacatcat gcggctcttg
     1381 cgcgcgcagc aagtcgaggc cggagacatc atcgccaagc gcggcgagcc cgctcattcc
     1441 atgtatttcg tcgccgccgg cgaagtggag atcgagctca agcacgaacg cgtccggctc
     1501 ggcgccggtc atttcttcgg cgaggtcgcg gcgctgcgcc gcgcacgcag atccgcgacg
     1561 gttactgcgc tcacgcggac aagcctgctc gtgctcgatg cccaagacct gcatgtgctc
     1621 atggagcggg aaccgcgcat cgcggaacat atccgcgaag tggctcgcac gcggctgggc
     1681 ggcgatgtcg tcacgcggaa gggcgatctt gtggtcgaag agctcgaaga ggcggaggcg
     1741 atccagcgcc cggcaggcag ccacggtcgc tcgtgacgcg aggctcaatg cgacaacatg
     1801 caacagaccg ggcttgcggt gagaagctcg cgcgtgacgc cgccgaaaat ccattcaccc
     1861 aatcggctgt gtccatagcc gccggcgacg attaaatctg ctttctcctc cgccacaacg
     1921 tgcagaagct cggccgcgac cggccctcgc ggccggcgcc agacttcggc tgccacgatc
     1981 acgcggtggc gcacgaggta ggccgcaacg tcggccagat tcttcttggc ctgccactgc
     2041 gattcgtcct cgccgacttc gacgagcaca acctccttgg cctgctgcaa gaacgggagc
     2101 gcgtcccgca ccgcgcggcg ggattcacgc gtatccttcc aagcgacgac cgcgtggcga
     2161 agcggcagcg ccgcgacggt gtcgggcacc agcagaacgg gacggcccgc ccgcagcacg
     2221 ataacccctg ggtcggcgtc gttctcgctg tgcctgcgac cgacgacgag gagatcggcc
     2281 gcccgtgctt cccgcaagag cagctcgccg ggagattcaa gggcgctgcg ccattcgata
     2341 tgccgcagcg agcggccctg ggcgcgaaac gtcttgcccc tctgctcgaa acgcgccatc
     2401 agcttttgaa cgtcgtactc gctggatttg gtcagtccga cgtctccggc cgtgaaggcc
     2461 ggccacagcg cgcaacccgc aacgccgata agggcggctt ggaagcggtc ggccagaccg
     2521 atcgccagtt caacccttcg cttcgaatcg tgctcgacgt cgacgtgcac catcatcgtt
     2581 gagaatgacg tcgcttgctc cgagccggcc gacctttccg atcgcgtggt gttcacggtg
     2641 gccacgagct ctgctccgct tcaccaccgc cagctgcggt gccttggccc gaatacatcg
     2701 agacgctgct gttgcttgct gtccagcgag tcgtaaaacg cgtcgaacgt cggtcgcaaa
     2761 ctcttcaccg cctgagacat tgtctcgagc cggttgtcca tcatctgcaa ttgggctttc
     2821 gtcgtcgcgg gcacgtcgga cttgcacgcc gtcgccatcg tcgcgatcgc cttgctcgag
     2881 gccgattgca gatcgttcag ggccgcgcgt tgggcttcgg tcggccgcac cacccgctcg
     2941 atccatcgca cttgccattc gtagaggccg accgaacgcg gcgtgcacag ccgcttatat
     3001 ccgcgcgggc ccatggttcc cggaccggtc aacacgccgg ggatgaggac gatggcgtcc
     3061 ttgtccaccg acggagaagg cgattgttgc gcggcgaccg gcgctccccc ggtcatgacg
     3121 cacacgccta ttgccaccag caccctgctc atccagttca tcatctgcgc gcacccttct
     3181 caggaaacca acgctgaaca atatcagttc gcaagacctt ccctatctaa agatgacacg
     3241 atgaagcagc cgaaagttga cgtggatcaa gggtggcgcc aaggctgcgc aacgcgtgcg
     3301 ggccggggct ggctgcaata tcgctcgagg ccgtctgcga aaggacagat gctactgctg
     3361 ctgaatggat tgaaggtaat cgatgatcgc gccaacgtcc tcagccgcga agacgaactc
     3421 cggcatgtcc ggatggccgg tggaaaggcc ctcagccaga gcctcggcaa ggccttcgat
     3481 cttgtatttg cgagagagca ttcggaaggg cggggcctcc gggtgcgggc tggcgccggt
     3541 gcggccgaca gcatggcaac gggcgcaatg ggtcgtcagc aagaattcgc cacgtcctgc
     3601 gggggtgccg gtcccttgtg ccaagacggg cgcaatgacg ctcatcgcca tcattgtgac
     3661 gagacaatgg aggatttgcc tacgcatccc taatcgtgtc cccttaccac atcgcgaatt
     3721 ttgtcgagca gcccattccc cagcagcggc ttctccacga tcgggatgtc ggctttttcg
     3781 gcttgctcgc gcagcaatcg gctggggtgg ctcgcaatca gaatcgcagg tgcggtgagg
     3841 tgtcggtcgc gcaactgggc gatcaggtcg agtccgctca tcgctggcat tttttggtcg
     3901 accaggtaac aatcgcagcg cgcgaggtcg cctgcgtcca gtagatcggt tgcggacacg
     3961 tagccacgga cggtcaggcc ttcgatttca agccagaatt tgagcgaatt tcgaaccgcg
     4021 agatcatcgc tgacgacgat gacgacgacc cccgagttcg aagcttcacc ctgacttttc
     4081 attgcttctg cttgcgcccg cgcctgcgca tggccgcatg gggtggcaac aagcagcaaa
     4141 atggagagtc ccgctttgac atggctcaaa tccgcgacgc gccggctggt ccgcgtcgga
     4201 cacagccccg gcgccctgca cgccagccga tcatggcgcc gcttaaactt atgaaatcct
     4261 tcacctaaga caggcagcgg cctgcgattt gcgccaacgg agaacgagcg agagggctga
     4321 ccgacgattc acagccgtca gccaaatctt gatcgggagc caatatgtgc atgcgtggga
     4381 ggctgacttc cggtggtcgg ttgaccggat tccgatggag tgcaggaacc agcagcaaac
     4441 ggtcgccctt ttgtgttcga ttgataattt ccgtggaagg tgcagcccct gaggaagccg
     4501 tcgcgaccga tacttcgata ccgatgacaa tagaaacgat cgcgagaagc acgctgcagc
     4561 cgctgacgag gtatctcaga gcccgacctg ttgatgacaa taaatcgctt cgaagcgatg
     4621 tctgcggcga catgacctga tccccccgat atgacttggt aattttagcg ggagcgccca
     4681 gacccgaaaa ttccgatatt gtccctccgg caagtcccgg aggctcgcga gctatcgcat
     4741 cgttcaagat tcgttcgaat cgagaactcc ggcgatcaag gccatgcgca cgagatcgga
     4801 caggcttgca gcttgcatct tgatcatgag attggcccga taggtctcga tcgttcgggg
     4861 gctgatgccg agatcgtagg cgatctgctt gttggcaagt ccggccacca atcccttgag
     4921 gacatcccgc tcgcggttcg acaatgccgc gaggcgggcc tcgatattgc tgcgctcggc
     4981 ctgacgcttt tgatcccggt ccccctggtc gagcgccgac cgcaccgacg cgaggagaac
     5041 ctcgtcctcg aagggcttct cgagaaagtc gacggcgcca agtctcatcg cttccacggc
     5101 gagcgggata tctccatgcg cggtgatgac gatgaccggc acggggtgtt tgagctctcg
     5161 caatcgtcgc aacaggtcga taccgctgag ctcgggcatc ctcacgtcgg tgatgatgca
     5221 gccggtccgc acggtgggcg caacttgcag gaacgcgacg gcggactcgt gcgttcgcac
     5281 ttcgatgccg gccgatccca gctggaaagc gagcgcctgg cgcaccgcct cgtcgtcatc
     5341 gatgacgtgg acgatggatt tgtcaggcgg catcgccaaa atcctcctcg ctcgcggccc
     5401 gtacggtgaa ccgaaagatc gttccaccgt cgggattggg ctccaccgtg atctgtccgc
     5461 cgtgggcctc gacaatggtg cggcagatcg acaagccgac ccccattccg tggcgcttat
     5521 tggtgacaaa gggttggaag agctgcgaca tcaggtcctg cgcgatcccg gaccccgtgt
     5581 cggccacgct gacggcgacc atctcgccgt cgccggcagc cgtggaaatg acgagctcgc
     5641 gtctttgcga cgcttccatg gcttcgacgg cgttgcgcaa caagttcaat aaaacctgct
     5701 gaatctgcac cttgtcgacg agaacgagat cgacggccgg atcgaattga aaccggacac
     5761 ggacgccctg atccttggtc ccgaccagcg ccaaggcgct cgcttcctca atgagcttct
     5821 tgacgctttc gacccgacgc tcgctgtcgc cccgcgccac gaattcgcgc aggcgacgga
     5881 tgatctgccc cgcgcgcaaa gcttgatcgc ccgccttgtc cagggcatcg cgcagcaagc
     5941 ccgagcggtc ctcgccacca ctttcgagca tacgtcgcga gcccttcatg taacccgtga
     6001 tggccagtaa cggctggttc aattcatgcg ccagcgcgga tgccatctgg cccatcgccg
     6061 tcaatcggga aacatgaacg agctccgatt gcagctcctg cagcctcgcc tcc
//
LOCUS       DATLJT010001598         1913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_618355,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001598 DATLJT010000000
VERSION     DATLJT010001598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618355"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VM910_36630"
     CDS             complement(<1..204)
                     /locus_tag="VM910_36630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI68069.1"
                     /translation="MTATVQDHIHASLSGVTRIYPATAGTRAVHALGQVDLDLKRGEF
                     FAVVGPSGCGKSTLLEVLAGLQQP"
     gene            complement(201..1166)
                     /locus_tag="VM910_36635"
     CDS             complement(201..1166)
                     /locus_tag="VM910_36635"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68070.1"
                     /translation="MNPRSIILIVAIGLALVRPVNAADLLVTQYKNDPSGAPYGIALE
                     KGFFQNHGLDITGIISGAGGGSSVRNAMASDLGYGDVTAAPVIAAAEQGQDVKIVSFT
                     SRSLADLVIIVMPDSPLKTAADLKGKKFGISNPKSLGEMMGVLVMEKAGLKQGDVQMT
                     ALGSLSGALTALENGVVDVTSIPIVLFRTRGGESKYRVLVSPKDLPLIPSQLGMATGQ
                     AIKEWPEKLRAIQVARREGTKFIYEHTDESIQILSKIYAPLPPKEVGIMVKELVEAKF
                     WTEGRIEMPLLEQTVHAMKGVGMLQNDVDLKKIVDGSFLPPDLQK"
     gene            complement(1470..>1913)
                     /locus_tag="VM910_36640"
     CDS             complement(1470..>1913)
                     /locus_tag="VM910_36640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68071.1"
                     /translation="IDMLHVPYKSSPPAIQDVLAGRVSMMFTDLTTGLPHVRSGALRA
                     LAVTRLQRSPLFPELPTLDEAGVTGFDMDSWAGIVAPAQTPPAIVTQLNRELRKIIDD
                     PDVKSKLGNVGFEAFSSSPKEFEGFVKVQLGKWGKMVKDAGIQPE"
BASE COUNT          350 a          606 c          575 g          382 t
ORIGIN      
        1 tggctgctgg aggccagcca gcacctccag tagcgtggac ttgccgcagc cagacggacc
       61 gacgacggcg aaaaactcgc cccgtttgag gtcaagatcg acctgtccga gcgcatgaac
      121 cgctcgcgtg ccggcggtcg ctggatagat ccgcgttaca cccgagaggg aggcgtgaat
      181 gtgatcctgg accgtggccg tcatttttgc agatcgggcg gcaagaatga gccgtcgacg
      241 atcttcttga ggtcgacgtc gttttgcagc attccgacgc ctttcatcgc atgcacggtc
      301 tgctcgagca gcggcatctc gatccggccc tcggtccaga acttcgcctc cacgagctcc
      361 ttcaccatga tgccgacctc cttcggtggc agcggcgcat agatcttgct gagtatctgg
      421 atagactcgt cggtgtgctc gtagatgaac ttggtgcctt cgcgacgcgc cacctggatg
      481 gcacgcagct tttccggcca ctcctttatt gcctggccgg tggccatgcc aagctgtgac
      541 ggaatgagcg gcaggtcttt cggactgacg agcacgcgat acttgctctc gccgccgcgg
      601 gtccgaaaca ggacgatcgg gatggacgtg acgtctacca cgccgttctc caacgcggtg
      661 agcgcgccac tgagactgcc aagcgccgtc atctgcacgt cgccctgctt gagtcccgcc
      721 ttctccatca ccagcactcc catcatctcg ccaagcgact tcggattgct gatgccgaac
      781 ttcttgccct tgagatcggc cgcggtcttg agcggtgaat cgggcatgac gattatcacc
      841 aggtcggcca gcgagcgcga ggtgaaactg acgatcttga cgtcctgacc ctgctcggcc
      901 gcggcgatga ccggggcggc ggtgacgtcg ccgtagccga gatcgcttgc catagcattg
      961 cgcaccgagc tgccgccgcc cgcgccggag ataatccccg tgatgtcgag gccgtgattc
     1021 tggaaaaagc ccttctcgag cgcgatgccg tatggcgcgc cggagggatc gttcttgtat
     1081 tgggtaacga ggagatcggc ggcgttgacc ggccggacca acgcaagacc aatcgccacg
     1141 atcaggatga tgcttctagg attcattatc atgtccgctc ctgagatgac ttcccgcgcc
     1201 cccggctgcg gctgcctgcc gtgatcgccc tagcgtagcc ggtgcagcgg ctgccgtata
     1261 ttggaacatt gacgcaggag cgtcagcgca tcgtactttg tagacacgcg tctactgaac
     1321 caagctaaca cgtcaagctg acacctaatg actttcaatc tgacttcgaa tacggcagtc
     1381 gaatttccgt tcacgtgagg tacggcagat tcaccacctg tgggccgctc ggcgcgaatc
     1441 tatgcggcga ggccaacgat gccctgctct cactccggct gaatgccggc gtccttgacc
     1501 atcttgcccc acttgccgag ctggaccttg acgaagccct caaactcctt gggcgagctt
     1561 gaaaacgctt cgaagccgac gttgccgagc ttcgacttga cgtccgggtc gtcgatgatc
     1621 ttgcgcagct ccctattgag ctgcgtcacg atcgccggcg gcgtctgggc cggcgcgacg
     1681 atcccggccc atgaatccat gtcgaagccg gttaccccgg cctcgtccag cgtcggcagt
     1741 tccgggaaca gcgggctgcg ctgcaggcgc gtcaccgcaa gcgcgcgtaa cgccccggat
     1801 cgcacatgcg gcagaccagt cgtgagatcg gtaaacatca tcgacacgcg gccggcgagc
     1861 acgtcctgga tggccggcgg tgagctcttg taagggacat gcagcatgtc gat
//
LOCUS       DATLJT010001599         5053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1996032,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001599 DATLJT010000000
VERSION     DATLJT010001599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996032"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VM910_36645"
     CDS             <1..168
                     /locus_tag="VM910_36645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HVI68072.1"
                     /translation="FATTGDADYHLRVVVSDMTAYNRFLDEFMFKLPGIRHVRTNVIL
                     KEIKYEVSLPL"
     gene            complement(174..752)
                     /locus_tag="VM910_36650"
     CDS             complement(174..752)
                     /locus_tag="VM910_36650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848430.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HVI68073.1"
                     /translation="MTQPTAKRFPTPAIDKLPDDIRTRLLAVQEKSGFIPNVFLALAY
                     RPDEFRAFFAYHDALMEKDGGLTKAEREMIVVATSAANNCEYCVIAHGAILRIRAKNP
                     LIADQIAINYRKADITPRQRAMLDFALKVALEGHLISEADFAVLAGHGFSDDDIWDIG
                     AIAALFALSNRMAHLTGMRANPEFYLMGRVPK"
     gene            complement(859..2595)
                     /locus_tag="VM910_36655"
     CDS             complement(859..2595)
                     /locus_tag="VM910_36655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HVI68074.1"
                     /translation="MTSLGRVISVRGSLARVGLLAKSQTPISEIRATVGRFVSLRCAA
                     STIVAMITDVTCEHLPTSDNYIATAGVDLLGEILNGDRPKFQRGVTNYPTIGDAVELI
                     SSQELRTIYTPNGSDQVDVGTLQQDRSVIAYVDIEEMLSKHFAVLGSTGVGKSTGVSL
                     LLNEILKARPNLRIFLLDVHNEYGRCFGDRSLVLNPRNLKLPFWLFNFEEIVDVLFAG
                     RPGVPEELDILAEVIPLAKGLYTQYQNADRMGLKRADPRAIGYTIDTPVPYRLVDLIS
                     LIDERMGKLENRSSRIIYHKLISRIETVRNDPRYAFMFDNANVGGDTMAEVISYLFRL
                     PANGKPMTVMQLAGFPAEVIDSVVSVLCRMAFDFGLWSDGVSPLLFVCEEAHRYAAAD
                     RNIGFGPTRKAVSRIAKEGRKYGVYLGLVTQRPAELDATIISQCNTLFTMRLANDRDQ
                     ALLRSAVSDAAANLLSFVPSLGTREVLAFGEGVALPTRLRFKEVPTHQLPRSEATIAT
                     SPSVTAGHDTHFVSAVLERWRGATSQRDVPNDPTVNQPSARTLSSYEAPMLQPSQGLD
                     PDRFSLLKKPLR"
     gene            2941..4563
                     /locus_tag="VM910_36660"
     CDS             2941..4563
                     /locus_tag="VM910_36660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HVI68075.1"
                     /translation="MDRSDVLIIGGGIAGITTALELLDGGKSVLLLDRDEESAFGGLA
                     RESFGGMFFVDSPEQRRQGIRDSAELAFSDWCSFAEFDPGDALPKAWAQAYVSRATAD
                     VYRWVRRYGVSFPPVVNWVERGEFHPGNSVPRFHVSWGTGKGLTEALMAALRAHPQAG
                     RLTLRFNRRVERLTRRGGRVDGAAGFDEKSGAPFEVAAEQVVVAAGGISGGDLSRVRQ
                     NWHRDWGTPPAELLSGSHRFADGKLHDATVEAGGTLTHLDRMWNYAAGVHHPRPRKPS
                     HGLSLVPPRSALWLNWRGERIGPQPLVSGFDTRRLVTDICRQERQYSWQLMNMRIALK
                     ELAISGAEHNPSIRDGKRLRFLRDITFGNRWLVNEMITNCRDFVAAGSLSELVDNMNA
                     LQGDKAVELATVQDAVARYDATILRGPDLMNDEQLRRIAALRKYRGDRMRICKFQPIN
                     DPKALPLIAVREFIISRKSLGGIKTDLESQVIDPAGQPIAGLYAVGEAAGFGGGGMHG
                     LRALEGTFLGGCILSSRIAAASIDGRTTFLKG"
     gene            4574..>5053
                     /locus_tag="VM910_36665"
     CDS             4574..>5053
                     /locus_tag="VM910_36665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI68076.1"
                     /translation="MKRTGAWLAVYALEQIGARFTFGIPGVHTTELYDALNSSNRITQ
                     VLVTHEGGASFMADAVGRLSDTIGVLTVVPAAGLTHAASGIGEAYLDGVPMIVISGGP
                     RTDTGHRYQLHQMDTHKFMGGLTKATFRVTRHDEVVPTLFKAYTIATSGEPGPVFVEM
                     "
BASE COUNT          962 a         1474 c         1670 g          947 t
ORIGIN      
        1 ttcgcgacca ctggcgatgc ggattatcat ctacgcgtgg tcgtcagcga catgaccgcc
       61 tataaccgct tccttgacga gttcatgttc aagcttccgg gcatccgtca tgtcaggacg
      121 aatgtcatcc tcaaggaaat caaatacgag gtgtccctgc cgctgtgaag ccgctatttc
      181 ggcacgcggc ccatgagata aaattccggg ttcgcccgca tgccggtcaa gtgcgccatc
      241 cggttcgaca gcgcgaacaa cgcggcaatc gcgccgatat cccagatgtc gtcgtcggaa
      301 aacccatggc ccgccagcac cgcaaaatcg gcttccgaaa tcaggtgccc ttcgagcgca
      361 accttcagcg caaagtcgag catcgcgcgc tgacgcggcg tgatgtcggc cttccggtag
      421 ttgatcgcga tctggtccgc aatcagcggg tttttcgcgc gaatgcgcag gatcgcgcca
      481 tgggcgatca cgcaatattc gcagttgttg gcggccgagg tcgcgaccac gatcatctcg
      541 cgctcggcct tggtcaggcc gccatccttc tccatcagcg cgtcatggta cgcgaaaaag
      601 gcgcggaact cgtccggccg ataggcgagc gccagaaaga cgttcgggat gaagccggat
      661 ttctcctgca ctgcgagcag acgcgtgcgg atgtcgtccg gcaatttgtc gatggcgggc
      721 gtgggaaagc gttttgctgt cggttgggtc atgaaaaggt tccggctcgg gccgcacagg
      781 cacggcctag gcagaacctt agtcgatgac gctgtccgtg caaggcgcac tggcgccttg
      841 ctggaatgct tgggaacgtc agcgcagcgg cttcttcaac agcgagaagc ggtcgggatc
      901 gagcccttgc gagggctgca gcatcggcgc ctcgtacgag gatagcgtcc gtgcggaagg
      961 ctggttgacg gtgggatcgt tgggaacgtc gcgctgcgaa gtggcgccgc gccagcgttc
     1021 cagcacggcg ctgacgaaat gggtgtcgtg gccggccgtc accgacggcg aggttgcaat
     1081 cgtcgcctcg ctgcgaggca gttgatgggt cggtacctcc ttgaagcgca gccgggtcgg
     1141 cagcgccacg ccttcgccga aggccagcac ttcgcgcgtg ccgagcgagg gaacgaagga
     1201 gagcaggttg gcggcggcat ccgagaccgc cgagcgcaac agcgcctggt cgcggtcgtt
     1261 ggcaaggcgc atcgtgaaca gcgtgttgca ttgggagatg atggtcgcgt cgagctcggc
     1321 gggccgctgg gtgacgaggc cgagatagac accgtatttg cgaccctcct tggcgatgcg
     1381 ggagaccgct ttgcgggtcg gtccgaagcc gatgttgcga tcggcggcgg cataacggtg
     1441 cgcttcctcg cagacgaaga gcagcggcga aacgccgtcg ctccatagcc cgaaatcgaa
     1501 cgccatgcgg cagagcaccg agaccacgga atcgatgact tcagccggga agccggcaag
     1561 ctgcatgacg gtcatcggct tgccgttggc aggcaaacgg aacagatagc tgatcacctc
     1621 ggccatggtg tcgccgccga cattggcgtt gtcgaacatg aaggcgtagc gcgggtcgtt
     1681 gcgcacggtc tcgatgcgcg aaatcagctt gtgatagatg atgcgcgatg agcggttctc
     1741 cagcttgccc atgcgctcgt cgatcagcga aatcaggtcg accaggcgat aggggacggg
     1801 cgtgtcaatg gtatagccga tcgccctggg atcggcgcgc ttcagcccca tgcggtcggc
     1861 gttctggtac tgggtataga ggcctttcgc cagcggaatg acctccgcca ggatgtcgag
     1921 ttcctcaggc acgccgggcc gaccggcgaa cagcacgtcg acgatctctt cgaagttgaa
     1981 tagccagaag ggcagcttga ggttgcgcgg gttaagtacc agcgagcggt cgccgaagca
     2041 gcggccatat tcattgtgca cgtcaagcag gaagattcgc aggttcgggc gtgctttgag
     2101 aatctcgttg agcagcaacg acacgccggt cgatttaccg acaccggtcg aaccgagcac
     2161 cgcaaagtgc ttggacaaca tttcttcgat atcgacgtag gcgatgaccg aacgatcctg
     2221 ctgcagcgtg ccgacatcga cctgatcgga cccgttcggg gtatagatcg tgcgcagctc
     2281 ctggctcgag atcagctcga cggcatcgcc gatggtcggg tagttggtga cgccacgctg
     2341 gaattttgga cgatcaccgt tgagaatttc gcccagcagg tcgacgccgg cagtggcaat
     2401 ataattgtcc gacgtcggca ggtgctcgca ggtaacgtcg gtaatcatgg ccacgatggt
     2461 ggacgcggcg cagcggaggc tgacgaagcg gccgacagtg gcacggattt cagagatcgg
     2521 cgtctggctc ttcgccaaaa gcccgacccg ggcgagggat ccacgcaccg aaatcacacg
     2581 tccgagggat gtcactttgg ttcctggtag ttcgcgagtg gggtcaggcc ctcactatgt
     2641 ccaaggtctt tggaaaaacg gttaagctgg tgaaacgggt actgcgtcaa atctgcgcca
     2701 ttttaggcgg gttgacctcg ggcggtcctg aggttctcga gaagtccccc aagaatcagg
     2761 cttttcgcaa acataccggc cgatcgcgct taaccgtcgg tttaccaaac ttgcgagcaa
     2821 gccagcggcc gttcggctag ccgaccctcg actccctcca gtggcccgat gccgatccac
     2881 atacggccaa aaccgtgtta ctcgcgggca gagagatgca aagagggaag cagggagcac
     2941 atggatcgca gcgatgtctt gatcatcggc ggcgggattg cgggtatcac cacggcgctg
     3001 gagctccttg atggtggcaa atcggtgctg ctgctcgatc gcgacgagga atcggccttt
     3061 ggcggtctgg cgcgcgaaag ctttggcggg atgttcttcg tcgattcacc cgaacaacgc
     3121 cgccagggca tccgcgattc cgcggaactg gcgttttccg actggtgcag ctttgccgaa
     3181 ttcgatcccg gggatgcttt gccaaaggcc tgggcccaag cctatgtgtc gcgcgcgacg
     3241 gccgacgtct atcgttgggt ccgccgctac ggcgtcagct ttcctcctgt cgtgaactgg
     3301 gtcgagcgcg gcgaattcca cccgggcaac tccgtgccgc gctttcatgt gagctggggc
     3361 acgggcaagg ggctcacaga ggctttgatg gctgcgctgc gcgctcatcc gcaggccggc
     3421 agacttaccc ttcgcttcaa ccggcgcgtc gagaggctca cccgccgcgg cggccgggtc
     3481 gacggtgcgg ccggcttcga cgagaaaagc ggggcgccgt tcgaagtggc ggcggagcag
     3541 gtggtcgtgg cagccggcgg tattagtggc ggcgaccttt cgcgcgtgcg gcagaactgg
     3601 caccgtgatt ggggcacgcc gccggcggag ctactgagcg gctcgcaccg cttcgccgac
     3661 ggcaagcttc atgacgcaac agtggaggcc ggcggcacgc tgacgcatct cgacaggatg
     3721 tggaactacg ccgcaggcgt gcatcatccg cgcccgcgca agccgtcgca cggtctatcg
     3781 ttggtgccgc cgcgctcggc actatggctt aactggcggg gcgagcgcat cgggcctcag
     3841 cctttggtgt cgggtttcga caccaggcgg ctggtaactg acatctgcag gcaggagcgg
     3901 cagtattcgt ggcagcttat gaacatgcgg atcgcgctga aggagctggc gatctcgggt
     3961 gccgagcaca atccctcgat tcgggacggg aagcgattga gatttctacg cgacatcaca
     4021 ttcggcaatc gctggctggt gaacgagatg atcacaaact gccgcgattt cgtcgccgcc
     4081 ggctcgctgt cggaactggt cgacaacatg aacgcgttgc aaggcgataa ggcggtcgag
     4141 ctggcgaccg tgcaagatgc ggtcgcgcgc tatgatgcga cgattttgcg cggtccggac
     4201 ctgatgaacg atgagcagtt gcgccgcatc gcagccttga gaaaatatcg gggcgatcgg
     4261 atgagaattt gcaaattcca gccgatcaac gaccccaagg cattgccgct gatcgcggtt
     4321 cgtgaattta tcatcagccg caagagcctc ggtggtatca agaccgacct ggagagccag
     4381 gtgattgatc cagccgggca acccatcgcg ggcctctatg ccgtcggcga ggctgccgga
     4441 ttcggcggcg gcggcatgca tggcttgcgc gcgctggaag gcacctttct cggtggctgc
     4501 atcctgtcca gccgcatcgc cgcggcctcg atcgacggcc gaacgacttt tctgaagggc
     4561 tagagggagc aatatgaaga gaacaggggc ctggctcgcg gtctacgcgc tggagcagat
     4621 cggggcgcgc ttcaccttcg gcattccggg cgtgcatacg acggagcttt acgacgcgct
     4681 gaacagctcc aaccggatca cgcaggtgct ggttacgcac gaaggcggcg catcgttcat
     4741 ggccgatgcg gtcggccgcc tgtccgacac gatcggcgtt ctgacggtgg taccggccgc
     4801 gggcctcact catgccgcaa gcggcatcgg cgaagcgtat ctggacggtg tgcccatgat
     4861 cgtcatctcg ggtggtccgc gcaccgatac cggacatcgc taccaattgc atcagatgga
     4921 cactcacaag tttatgggcg gattgaccaa ggcgaccttc cgcgtcacgc gtcatgacga
     4981 ggtcgtgccc acactgttca aggcctacac gattgcgacc tccggcgagc ctggacccgt
     5041 gttcgtggaa atg
//
LOCUS       DATLJT010001600         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_619010,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001600 DATLJT010000000
VERSION     DATLJT010001600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619010"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VM910_36670"
     CDS             <1..633
                     /locus_tag="VM910_36670"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI68077.1"
                     /translation="RGQHIQIAMRDAMLNYCRTPMSRQAARDDQLPRGGNTVPGTAPS
                     GLYKCAPEGLDDLCYIFTSRGNEEHWRRLLKAISREDLLDDPRMVDGVTRAKNREAVD
                     AAITEWTSKRTKHEVTRIIAGAGVPCGAVLTTLELMHDPDLHKRGMMQKIEHPLRGQV
                     IVPGWPLRMSDSKVPLKCAPILGADSEAIYGEWLGCSPDEVRDMRQAKVI"
     gene            complement(716..1687)
                     /locus_tag="VM910_36675"
     CDS             complement(716..1687)
                     /locus_tag="VM910_36675"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68078.1"
                     /translation="MRRRDFIAIVGGAATWPMAVSAQQAGKLPTIGFLGADPAGFAPW
                     TAAFVARLRELGWVDGRTVAIEYRWSEGRTERYAEIAAEFVRQKVDVIVTVGSAVPTV
                     RQATSIIPIVFAVAIDPVRSGLVSSLANPGGNVTGLSLQATNLAGKRLEILRELIPEL
                     RRLAIIFNGGNDQTMLEMSDTEAAARELKLDVALLEIWRMQDIAPAFEKLNSQADALY
                     VVVDQLIVTNYNRILTFALSTRLPMIYSTRDFVRSGGLMSYGPSYVDLFRRAGDYVHR
                     ILQGAKPADMPVEQPTRFELVLNLTTARALGLRVPEPFLLRADEVIE"
BASE COUNT          435 a          672 c          619 g          355 t
ORIGIN      
        1 cgcggccagc acatccagat cgccatgcgc gacgcgatgc tgaactactg ccgcacgccg
       61 atgagccgcc aggccgcccg cgacgaccaa ctgccgcgcg gcggaaacac ggtgccgggg
      121 accgcgccct ccggcctcta caaatgcgcg ccagaggggc tcgacgacct gtgctacatc
      181 tttacctcgc ggggcaacga ggagcattgg cgccgcctgc tgaaggcgat tagccgcgag
      241 gacctgctcg acgatccgcg tatggtcgac ggcgtcaccc gcgccaagaa tcgggaggcc
      301 gtggatgcgg ccatcactga atggacatcg aagcgcacca agcatgaagt aacccggatc
      361 atcgccggcg ccggcgttcc ctgcggcgcg gtcctcacca cgctcgaact gatgcacgac
      421 ccggatctgc ataagcgggg aatgatgcag aagatcgagc atcccctgcg tgggcaggtc
      481 atcgtgccgg gttggccgct gcggatgtcg gacagcaagg tcccgctgaa atgcgccccg
      541 attctcggcg cggattccga ggcgatctac ggcgagtggc tcggctgttc ccccgacgag
      601 gtccgggaca tgcggcaagc caaggtgatc tgaggactgc gatccgggcc gcgctgctgc
      661 cgccgacatc gcgcatactt gccatgggcc acgaacggac tcacgccgcc acgccctatt
      721 cgatcacttc gtccgcgcgc agcaagaacg gctcagggac acgaaggccg agggctcttg
      781 ccgtcgtgag attgaggacc agttcgaacc tcgtcggctg ctcgaccggc atatcggcgg
      841 gcttcgcacc ttgtagaatc cgatgcacgt aatcgcccgc acgccgaaac aggtctacgt
      901 agcttggccc gtaagacatg agtcctccgg atcggacgaa atcgcgggta ctgtagatca
      961 tcggcaatcg cgtgctgagc gcgaaggtca ggatgcgatt ataattggtg acgatgagct
     1021 ggtcgaccac gacatagagc gcgtccgcct gggagtttag cttctcgaat gcaggagcaa
     1081 tatcttgcat acgccaaatt tcgagaagag cgacgtcgag tttgagttcg cgcgccgcgg
     1141 cctcggtatc gctcatctcc agcatcgtct gatcattgcc gccattgaaa atgatggcca
     1201 atcgacgcag ctccgggata agctcgcgca atatttccaa tcgtttcccc gcgaggttgg
     1261 tggcctgcag cgacaatccg gtcacgttgc cgcccggatt ggccagactg gagacaaggc
     1321 cgctgcgcac cgggtcgatc gccaccgcaa atacgatggg gatgatactg gtcgcttgcc
     1381 ttaccgtggg aacggcgctt ccaacggtaa caatgacatc caccttctgg cggacaaact
     1441 ccgccgcgat ttccgcgtaa cgctcggtgc gcccttcgga ccatcgatac tcgatcgcaa
     1501 ctgtacggcc atcgacccag ccgagctcac gcaggcgcgc cacgaaagca gcggtccagg
     1561 gagcaaagcc ggcagggtct gccccgagaa atcctatcgt cggcagctta ccggcctgct
     1621 gcgcactgac agccataggc caagttgctg cgccgccgac gatcgcaatg aagtcgcgtc
     1681 gcctcatgcc cggccccagt actcacaccc cttaaactag cacaccggaa accggaagct
     1741 tgcgacatga tgtccgcctc ggggtcacgg cgtttgtcat ctccggtcag acccggcggt
     1801 gacgcgacgc tacttcaatc ccgttcccca gcgccggtgc aggaatcgtt cccagggaga
     1861 aaataggatc ttgtcggcca ttaatccgat cagcacgatc acaatcatca ccccgatcac
     1921 ctggtccatg gccgacaatt caggagatgg ccgagaccga atccggtcag gatggtgacg
     1981 aagatttcgg ccgccataag cgatcgccag gcgaacgccc aaccctgctt catgccgctg
     2041 accacataag gaagcgatgc cggcaggatc acgcgaatcc a
//
LOCUS       DATLJT010001601         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3836254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001601 DATLJT010000000
VERSION     DATLJT010001601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2103)
                     /locus_tag="VM910_36680"
     CDS             complement(<1..>2103)
                     /locus_tag="VM910_36680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018070860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68079.1"
                     /translation="AASKAQPLLWISSTFFEEAEYHFYGALAKAAWCECAPADERPQH
                     LDALATHYRQLQIWAKNCPENFADRAALVGAEIARIEGRVVDAEQLYEQAIHSAQESG
                     FVHGEALANELASRFYAARGFGTIAHVYMQDARYGYLRWGADGKVRQLEERYPYLRAE
                     EPAPGPTTTISTPVERLDLATVIKVSQAASGEIVLDKLIEMVMRTAIEQAGAERGVLI
                     LSDGGEERIAAEAATTGAAPKLEFRDVPVSTAMLPESILYHVLRTRESVFLDDAAAES
                     PFAADPYIRQHRARSILCFPLMNQAKLTGALYLENSLTAGVFSPARIAVLKLLASQAA
                     ISLENARLYRDLAEREAKIRRLVDANIIGIVVWNADGDILEANDEFLRMVGYEREDLV
                     SGRVSWRDLTPSEWLERDERALAEIAATGRAQPFEKEYIRKDGSRVPVMLGATASEAS
                     RKEGVAFVLDLSERKHAEEKVRESEQRYRAVQTELAHASRVATLGQLTASIAHEVRQP
                     VGATVTNAQAALRWLGAQPPNLQEVRQALTRIVKDGDRASEVVGRIHGFIKKTPSRKE
                     AFEINASILEVIALTGGEAVKHGISVRTQLAEGLPVIQGDRIQLQQVILNLMMNAVEA
                     MSGVPEGPRELLISTDKAESDGVFVAVRDSGPGLSPASRERAFEAFYTTKSGGLGMGL
                     SICRSIIEAHGGRLWSMA"
BASE COUNT          380 a          695 c          616 g          412 t
ORIGIN      
        1 ttcgccatag accataatcg tcccccgtgc gcttcgatga tcgaacggca gatcgacaga
       61 cccatcccca aaccaccaga cttggtggta tagaaggcct caaaggcacg ctcgcgactc
      121 gctggtgaga ggcccggacc cgaatcccgc accgcgacga acacgccgtc cgattcggcc
      181 ttgtcggtgc tgatcagaag ctctcgcgga ccttctggaa cgccgctcat tgcttcgacg
      241 gcgttcatca tcaagttcag gatcacttgt tgcaattgga tccgatcacc ttgaatgacc
      301 ggcaatcctt ctgcaagttg cgtccgtacc gagatgccgt gtttcacggc ttcgccaccg
      361 gtgagggcaa tgacctcgag gatcgatgcg ttgatctcaa aggcctcctt ccgcgagggc
      421 gtcttcttga tgaagccgtg gatccggccg acaacctcgc tggctcgatc accatctttg
      481 acgattcgag tgagcgcctg ccgcacttct tgcagattcg gcggttgggc gcccagccag
      541 cgcaaggcgg cctgcgcgtt ggtgacggtc gccccgaccg gctgtctgac ttcgtgagcg
      601 atcgaggccg tgagctgccc cagggttgcg acacggctcg cgtgtgccag ttccgtctgc
      661 accgcgcgat agcgctgctc gctctcgcgg actttttcct ccgcgtgctt gcgctcgctc
      721 agatcgagca cgaaagcgac accttccttc cggcttgctt cggaagcggt cgcaccgagc
      781 atgacgggca cgcggctgcc gtccttcctg atgtactcct tctcgaaagg ctgcgcgcgc
      841 cccgtcgccg cgatctctgc cagagcccgt tcgtcgcgct cgagccattc tgacggcgtc
      901 agatccctcc agctcacgcg acccgagaca agatcctccc gctcgtatcc caccatgcga
      961 agaaattcgt cattggcctc aagaatgtcg ccatcggcat tccagacgac gatcccgatg
     1021 atgttggcgt cgaccaggcg ccggatcttc gcttcgcgct ccgcaagatc gcggtacagg
     1081 cgggcattct ccagcgaaat cgcagcctgc gaggccaaca gcttcagcac ggcaatcctg
     1141 gccgggctga acacgccggc agtcagactg ttttcgaggt agagtgcgcc ggtgagcttc
     1201 gcctgattca tcaacgggaa gcagagaatt gaacgggcgc gatgctggcg gatataggga
     1261 tccgcggcaa acggggactc agccgcggcg tcgtcaagaa agacgctctc ccgggtgcgc
     1321 aggacatgat agaggatcga ctccggtagc atcgccgtgc taacgggaac atcgcgaaac
     1381 tccaacttcg gcgcggcgcc ggtggtcgcc gcttccgccg cgatccgttc ttcgccgccg
     1441 tccgacagaa tcaacacacc tcgttcggcg cccgcctgtt cgatggctgt acgcatgacc
     1501 atttcgatca gcttgtccag cacgatctcg cccgaggcgg cctgcgacac cttgatcact
     1561 gtcgcgaggt cgaggcgctc gaccggcgtt gaaatcgtgg tcgttggccc cggcgcaggc
     1621 tcttccgccc tcaagtacgg atacctctcc tcgagttgcc gcaccttccc gtcggcgccc
     1681 caacgcagat agccgtagcg ggcgtcctgc atatacacat gcgcaatcgt cccaaaaccg
     1741 cgcgccgcgt aaaagcggga cgcgagttca ttggcgagtg cctcaccgtg gacaaagccg
     1801 ctttcctgcg ccgagtggat ggcctgttca tagagctgtt cggcatccac tacgcggcct
     1861 tcgatccggg caatctcggc tccgaccagc gcggcgcgat ccgcgaaatt ctccgggcag
     1921 tttttcgccc agatctgaag ctgccggtaa tgtgtcgcga gcgcgtccag gtgttgtggc
     1981 cgctcgtcgg ccggggcgca ctcgcaccag gcggcctttg ccaacgcgcc gtaaaaatga
     2041 tattcggctt cctcaaagaa cgtggaagag atccagagca gcggttgcgc cttcgacgcg
     2101 gcg
//
LOCUS       DATLJT010001602         3650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4522264,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001602 DATLJT010000000
VERSION     DATLJT010001602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4522264"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VM910_36685"
     CDS             <1..199
                     /locus_tag="VM910_36685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68080.1"
                     /translation="TAKTPGLTVPPTLLARADEIIEQEICCNANVRFWHKADLARGHP
                     TCPLLGQSGHGATAAGVSTGR"
     gene            400..1401
                     /locus_tag="VM910_36690"
     CDS             400..1401
                     /locus_tag="VM910_36690"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68081.1"
                     /translation="MSGMGRREFITVLGGAAVAWPLAARAQQADRMRRIGVLSGLGEN
                     DPEGHARVAALREGLQKLGWTEDRNVRIDIRWGTPDVESMQRFASELVALQPDLIVTQ
                     NTPGTAAMLQQTRAIPIIFVNVVDPVGSSFVASFPRPGGNVTGFVSLEPTIAGKWLEL
                     LKQVQPRLNRVAFLFNPTTASYAEYYLNPFKVAALSFSVEAIAAPIRDMSELESAIAA
                     LARGPNGGLIVMPDSFLIAQRMAVTSAAARYRLPAVYPFRYFTEVGGLLSYGSDQLDN
                     YRRAASYADRILKGEKPSELPVQVPVKFEMTVNLKSAKALGLEVPPTLLAFADEVIE"
     gene            1398..2372
                     /locus_tag="VM910_36695"
     CDS             1398..2372
                     /locus_tag="VM910_36695"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68082.1"
                     /translation="MTRRAFITLLGGTAAWPLAARAQQPSKLPTIGLLGAGTRSGWTD
                     WVAAFVQRLQELGWIEGRTVAMEVRWAEGRSERYIEIAAEFVRLKVDVIVTVGSAVDA
                     VKQATSVIPVVFAAAVDPLGTGLVASLARPGGNITGLSMQSADLAAKRLELLREVLPD
                     LRRLAVMGNVNYPAAVLEIGEVQAAGRTLGVDVDKFEIRRGQDIAPAFEAFKGGSQAL
                     YLCADALLNAYQNRINILALGARLPTIYPSRNFLQSGGLISYGVNNADLFRRAADYVD
                     KILRGAKPSNLPVEQPTKFELVFNLTVAKILGLEIPPTLLARADEVIE"
     gene            2391..3368
                     /locus_tag="VM910_36700"
     CDS             2391..3368
                     /locus_tag="VM910_36700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68083.1"
                     /translation="MKLPRRNFLHLAAGAAALPAVSRFARAQTYPTRPVRIIVGFPAS
                     GGTDITARLIGQWLSERLGQQFVIENRPGAGTNIATEAVVRAPADGYTLLLVTASNAF
                     NATLYDKLNFNFIGDIAPVAGIMRVPSVMLVHPSVPAKTIPELIAYANANLGKINVAS
                     GSIGGPGHVAAELFKMMTGTDMPLVPYRGGGPALVDLLAGQVQVGFITAAASIEYIKT
                     GRLRALAVTTATRSETLPNIPTVGEFVPGYEASFWYGIGAPRNTPAKIIDTLNKQVNA
                     ALDDPKMNARLADLGGVALPGSPAEFGKLIADETEKWAKVIKFAGIKAD"
BASE COUNT          705 a         1133 c         1118 g          694 t
ORIGIN      
        1 gaccgccaag acaccgggcc tgaccgtgcc gccgacgctt ctcgcccgcg ccgacgagat
       61 catcgaacaa gaaatctgct gcaatgcaaa tgtccgcttt tggcacaaag cggacttagc
      121 gcgaggtcac cctacttgtc cgcttttggg gcaaagcgga catggcgcga ccgccgctgg
      181 cgtatcgacc ggtcgctgat gacccaaagc agacataggc cggatctaaa ttccgcagtg
      241 caacagtctc ctgccgtacc ttgacgtgct atccttcgag gggcacggca gatcaaacac
      301 gattcagaac atttcaggcc tggcccaagg acgtttcgac ctctctgcgg caggttgagc
      361 gtgaggtaga ccggcggcgc ccaggcggga ggaagcccga tgtcgggcat gggacggcgc
      421 gagttcatca cggttctcgg cggcgcggct gttgcatggc cgctcgcggc gcgcgcgcag
      481 caggccgacc ggatgcggcg aatcggggtg ctgagcgggc ttggcgagaa cgatccggag
      541 ggccacgccc gcgtcgcggc gttgcgggag gggctccaga agcttggctg gacggaagat
      601 cgcaacgtcc ggatcgatat ccggtgggga acgcccgacg tggagtcgat gcaacgattc
      661 gcgagcgaac tcgtcgcgct tcagcccgac cttattgtta cgcaaaacac cccgggcact
      721 gctgcgatgc tgcagcaaac gcgcgccatc ccgatcattt tcgtgaacgt cgtcgatccg
      781 gtcggcagta gcttcgtggc aagcttcccg cgcccaggcg ggaacgtcac aggtttcgtc
      841 agtctcgagc cgacgatcgc cggcaagtgg ctagaactgc tcaagcaggt tcagccgcga
      901 cttaatcggg ttgccttctt gttcaacccg acaacagcat catatgccga atattacctg
      961 aacccgttca aagtcgccgc gctatctttc tcggtggaag ccatcgcagc acccattcgc
     1021 gacatgtccg agctggaatc cgccatcgcc gccttggcgc gcggaccgaa tggcggcctt
     1081 atcgtgatgc ccgattcctt cttaattgca cagcgtatgg cggtcacatc agcagctgct
     1141 cgctatcgtc tccctgccgt ctacccgttc cgttacttca ctgaagtcgg cggcctgtta
     1201 tcctacggca gcgaccagct cgacaattat cggcgggcag cgtcttatgc cgatcgcatt
     1261 ctcaaaggcg agaagccgag cgagcttcct gtccaggttc cagtcaaatt cgaaatgacg
     1321 gtcaacctca agtccgcgaa ggcgctgggt ctcgaggtgc cgccgaccct gctcgccttc
     1381 gccgacgagg tgatcgagtg acgaggcgcg cattcatcac gctgctcggc ggcacggcgg
     1441 cctggccgct cgcggcgcgc gcgcagcagc catcgaaact accgaccatc ggactcttgg
     1501 gcgcgggcac gcgttcaggc tggaccgatt gggtcgctgc ttttgtgcag cgcctgcagg
     1561 aactcggctg gatcgaggga cgcaccgtgg cgatggaggt tcgctgggca gagggacgct
     1621 ccgagcgcta catcgagatc gcagctgagt ttgtccggct caaggtcgat gtcattgtca
     1681 cggtaggaag cgcagtcgac gctgtaaagc aggccacatc ggtcatcccc gtggtcttcg
     1741 cggcggctgt ggacccactt gggaccggtt tggtcgcgag tctggcgcga ccgggcggca
     1801 acatcaccgg cctgtcgatg cagtcagcgg atcttgctgc caagcgactg gaattactac
     1861 gcgaagttct acccgatctc cgccggttgg cggtcatggg caatgtcaat tatcccgcag
     1921 ccgtactgga gataggcgag gttcaggcag cgggccgcac gctcggcgtc gacgttgaca
     1981 agttcgaaat ccggcgcggc caggatatcg cgccggcctt cgaggcgttc aagggcgggt
     2041 cgcaagccct gtacctgtgt gccgacgcgc tgttaaacgc ctaccagaac cgcattaaca
     2101 tcctagcact aggcgcccga ctgcctacga tctaccctag tcggaatttt ctccaatcgg
     2161 gcggtctcat atcttatgga gtaaacaacg cggacttgtt tcgccgcgcc gcggactatg
     2221 tcgataagat tctgcgcggg gccaagccga gcaaccttcc tgtcgagcag ccgaccaaat
     2281 ttgaactcgt cttcaatctg accgtcgcca agatacttgg cctcgaaatt ccgccgacgc
     2341 tgctcgcccg cgccgacgag gtgattgagt gatgtgaagg gaggttcgaa atgaaacttc
     2401 cccgccgaaa ttttctgcat ctggccgcgg gcgctgccgc gctcccggcc gtgtcgcgct
     2461 ttgcgcgggc gcaaacctat ccaacgcggc cggtccgcat cattgtcggc tttcccgcca
     2521 gcggcggcac tgacatcacc gcgcgcctga tcggccaatg gctctcggag cggctcggcc
     2581 agcaatttgt tatcgagaac cggccgggcg ccggcactaa tatcgccacg gaggccgtgg
     2641 tgcgcgcgcc cgcggacggt tacacgctgc tcctcgtcac ggcgtcgaac gcgttcaacg
     2701 cgacactcta cgacaagctc aacttcaatt tcataggcga catcgcaccg gttgcaggca
     2761 tcatgcgcgt gcccagcgtc atgctggtgc atccatcggt tccggccaag acgatccccg
     2821 agctcatcgc ttatgccaac gctaatctag gcaaaatcaa cgttgcgtca ggctccattg
     2881 gaggcccagg ccacgtggcg gccgagctat tcaagatgat gacgggaacc gacatgcccc
     2941 tcgtgcctta tcgcggcggc gggcccgcgc tcgttgatct gctcgccgga caggtgcagg
     3001 tcgggttcat caccgctgcc gcatcgatcg agtatatcaa gactggcaga ctgcgcgcgc
     3061 tagcggtaac gactgcgacg cgctcggaga cgctgccaaa cattccgact gtgggcgagt
     3121 tcgtgccggg ctacgaggca agtttttggt acgggatcgg cgcacccagg aacacgcccg
     3181 ccaagatcat cgacacactc aacaagcagg tcaacgcggc tctcgacgac cctaagatga
     3241 acgcccgtct cgctgacctc ggcggcgtgg cgctgccggg atcgccggct gagttcggaa
     3301 agctgatcgc cgacgaaacc gaaaagtggg ccaaggtgat caaattcgcc ggcatcaagg
     3361 cagactgagg cgggaaggcg cggatattcc ataacgtccc gttacgcgaa tgacgcaatc
     3421 acgggattgc tctttcggag agtctaccga tgtccgctcg acgaccgctt tcgggcaaac
     3481 cggacattgc agccgaaatg gccgtagggc cgcttttgat gctgtggacg gcgcccactc
     3541 tgcggcatca aggtgccata gggtggcttc gtcgaaacga accactttga ggggagccgt
     3601 ccatgggcga ggttagcacg atcggtctcg atattgcgaa atcggttttt
//
LOCUS       DATLJT010001603         1623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_157560,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001603 DATLJT010000000
VERSION     DATLJT010001603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157560"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VM910_36705"
     CDS             complement(<1..561)
                     /locus_tag="VM910_36705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI68084.1"
                     /translation="MSDSMTPLDRARQAMIATGQWDASQAMGRSWPIGCVALEITQRC
                     NLDCTACYLSEHSQAVKDLPLEEVFRRIEMIRARYGAHTDVQVTGGDPTLRRTDELLA
                     IVRRIEGLGMRPSLLTNGIRASRELLVGLAEAGLVDVAFHVDITQRRKGYRTESELNA
                     LRRTYIERAGGLPLSVFFNTTVCDENF"
     gene            complement(713..1042)
                     /locus_tag="VM910_36710"
     CDS             complement(713..1042)
                     /locus_tag="VM910_36710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68085.1"
                     /translation="MFENRRDPGLSREFVGAWVVTLVVGVLGLIVTSHHVPDVRDRVV
                     VPQWYDPPVAGAEPSESGAPGPRGGAVTTSPADNLPPICDAVADSASVAPAPSPGTAA
                     NGSELPC"
     gene            complement(1118..1423)
                     /locus_tag="VM910_36715"
     CDS             complement(1118..1423)
                     /locus_tag="VM910_36715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68086.1"
                     /translation="MAVVSLSRDEVVKFVARFGDHFTPWELRRGPSQTHLSGSAFAVS
                     GEPQHRICGFSTVGHAGVVSHWDLPLTFGFLVHLSERETTAIAGTLLSARADHAIVT"
BASE COUNT          309 a          512 c          508 g          294 t
ORIGIN      
        1 gaagttttcg tcacagacag tcgtgttgaa gaacaccgag agcggcaagc cgccagcccg
       61 ctcgatatac gtccggcgga gcgcattgag ctcgctttcg gtgcgatacc ctttgcgcct
      121 ttgcgtgatg tcgacatgga atgcgacatc gaccaaaccg gcctccgcca gaccgacgag
      181 cagctcgcgc gaggccctga tgccgttcgt cagcaatgac ggccgcattc ccagcccctc
      241 gatgcggcgc acgatcgcca gcagctcatc ggttcggcgc aaggtcgggt cgccgccggt
      301 gacctggaca tccgtatggg cgccgtagcg ggcgcggatc atctcgatgc ggcgaaagac
      361 ctcttcgaga gggaggtctt tgaccgcctg ggaatgctcg gacagatagc aggcggtgca
      421 atcgagattg cagcgctgcg tgatctcgag tgcgacacag ccgatcggcc agctccgccc
      481 catcgcctgg ctcgcatccc attggccggt tgcgatcatc gcctggcgtg cccggtcgag
      541 cggggtcatc gaatcagaca tggccgaaat cggcggcagg ctcattcctc caacggtcga
      601 tcgaatgtca atagccggcg gttccttcga tcgcccgtgg agcttacgcc ttctggcccc
      661 gcccggggtc gagcactggc cacgcattct agcggacccg cttcgatgca gctcagcatg
      721 gtagttcaga cccattggcg gcggtcccgg gcgacggagc cggtgcaacg gacgctgagt
      781 cggcgactgc gtcgcagatc ggcggtaggt tgtccgccgg tgaggtggtc acagcgccgc
      841 cacgaggtcc tggcgcccca ctctccgacg gttccgcccc ggcaacgggc gggtcgtacc
      901 actgcggcac cacgacccga tcgcgcacat ccggcacatg atgcgaggtc acgataaggc
      961 cgagcacgcc gacaacgagg gtgaccaccc aggcgccgac gaactcacgg ctcaacccgg
     1021 gatctcgtcg attttcgaac atgacgccac tcccgatacg tccgcagctc ggcgaaatac
     1081 ttccgtcatc tatagattcg tccgaggagc cggcgggcta cgtcacaatc gcgtgatcgg
     1141 cgcgagcgct caggagagtc ccagcaatgg ctgttgtttc tctttcgctg agatgcacaa
     1201 ggaagccgaa tgtcagtggt aaatcccagt gggacaccac gccggcatga ccgacggtgc
     1261 tgaagccgca gatccgatgc tgaggctcgc ccgatacggc aaaggcagat ccgctcaggt
     1321 gtgtttgcga ggggcccctt ctgagctccc agggggtaaa gtgatctcca aatcgagcga
     1381 cgaattttac cacctcatcg cgcgaaagag aaacaacagc cattgctggg actctcctca
     1441 gcgctcgcgc cgatcacgct attgtgatgt agcccgccgg ctcctcggac gaatctatag
     1501 atgacggaag tatttcgccg agctgcggac gtatcgggag tggcgtcatg ttcggaaatc
     1561 gacgagatcc cgggttgagc cgtgagttcg tcggcgcctg gatggtcacc ctcgttgtcg
     1621 gcg
//
LOCUS       DATLJT010001604         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2916068,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001604 DATLJT010000000
VERSION     DATLJT010001604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916068"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VM910_36720"
     CDS             complement(<1..372)
                     /locus_tag="VM910_36720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HVI68087.1"
                     /translation="MSRVQDVYPEGRVSLREVGLRDGLQLVKTFPSTAAKQRWLREEY
                     AAGVRHFEVGSFLPAKTFPQFADVRDIVAAVAALPGAHGIALVLNERGANEALASGVA
                     EIASVVSATEEHSQANANRSRE"
     gene            complement(369..1541)
                     /locus_tag="VM910_36725"
     CDS             complement(369..1541)
                     /locus_tag="VM910_36725"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVI68088.1"
                     /translation="MEGSGLPLEGVRVVEMTHMVMGPTCGMILAQLGAEVIKVEPPAG
                     DKTRSLGGMGTSFFPLFNRGKRSVVLDFAREEDRKTMHRLLETADVFLENFRDGQLEK
                     QGMGAEELRKQHPHLIVAGHKGFLSGPYDHRPAHDEVVQMMSGLAAMTGTKEKPQRVG
                     SSANDIMGGMFGAISILAALYQKRGGAPSGADIRIGLFENCLFLVAQHMVEFEMTGNR
                     PRSMPEREHAWPIYDIFDTAGGNRIFIGVVTEGHWQSFCREFGLQEFLDDPSLRTTTD
                     RILARSRIIPRVADVIRCWNVAELSEKLDRLNICFSPINRPEDLFGDPHVLRPGGLVN
                     NFNADGAVFRVPALPVQWNGANIGEGLKVPPLGADTAAIRTELATKDNPKAERKRA"
     gene            1724..3133
                     /locus_tag="VM910_36730"
     CDS             1724..3133
                     /locus_tag="VM910_36730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI68089.1"
                     /translation="MTTVSVATGTDVAAASDGPSEISQRLERLPASSYLWRLVILLSL
                     GGCFEIYDLFLTGYIAPGLNRSGLLTTTTQAFFGFSGIGAFVAATFAGLFAGTFFLGF
                     LADRFGRRAIFTFALLGYSAASVIMACQTTSGGLLLWRFLAGIGIGVEIITVDAYITE
                     LVPSRMRGSAFAVNQAVMFIAVPIVAFLAWWLVPLTPHGIDGWRWVVLIGAAASMVIW
                     ILRLYLPESPLWLARHGRNSEAIDIVRKLEAATGADQRPGRAAEARASVAIKPAGFAD
                     LLRAPYLSLVVLFMVFNFCQAFGFYGFANWVPTLLVEKGITVTKSLEYSFIVAFAYPI
                     APLVAASFADRFERKWIICGACTAIIIFGLSFAQLVQPALLIAFGVLLTAANMTMSYA
                     YHAYQTEVFPTAIRARAAGLVYSMSRISATFSGFIVAFMLKEGGVGGVFGLITAAMLI
                     VIVTLAAFGPNVRGKPLDA"
     gene            3210..>3761
                     /locus_tag="VM910_36735"
     CDS             3210..>3761
                     /locus_tag="VM910_36735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HVI68090.1"
                     /translation="MIDAAVKALSQILSPPMRSILWRSIGLALVLITVLAVGLQRLLS
                     WFATYGEGWAETMLGPGFHTPLNVLAWIVSIAAGLGVVLGGIFLMPAITSLVASFFVD
                     DVADLVEREHYPAERPGVALPLGLAMSEGIRTALLTIVVYLVALPFVLVAGAGFLVFF
                     IATAWLLGKQYFELAAMRFRSPAE"
BASE COUNT          602 a         1202 c         1160 g          797 t
ORIGIN      
        1 ctcccgcgag cggttggcat tggcctggct gtgctcctcg gttgcggaga cgacggaggc
       61 gatttcggcg acgcctgagg ccagcgcttc gttcgcaccg cgctcgttca acacaagcgc
      121 aatgccatgg gcgccgggca gcgcggcgac ggcagcgacg atgtcgcgca cgtcggcaaa
      181 ttgcggaaag gtctttgccg gcaggaacga gccgacctcg aagtgcctga cgccggcggc
      241 atattcctcg cggagccagc gttgtttcgc cgccgtcgag ggaaaggtct ttacgagctg
      301 gaggccgtcg cgcaagccga cttcgcgcaa gctcacgcgg ccttcgggat agacgtcttg
      361 tactctgctc atgcgcgctt cctctcggct ttaggattgt ctttggttgc aagctcggtg
      421 cggatcgccg cggtgtcggc gccgagcggc ggcactttca agccttcgcc gatattggcg
      481 ccgttccatt gcaccggcag cgccgggacg cggaaaactg caccatcggc gttgaaattg
      541 ttgacgagcc cgcccggccg caacacatgc ggatcgccga acaggtcttc cgggcgattg
      601 atcggcgaga agcagatatt gaggcggtcg agtttttcgg aaagctcggc cacgttccag
      661 caccggatca cgtcggcaac ccgcggaatg atgcgcgagc gcgccaggat ccggtcggtg
      721 gtggtgcgca agctcggatc atccaggaat tcctgcagac cgaactcacg acagaagctc
      781 tgccagtggc cttcggtgac cacgccgatg aagatgcggt tgccgcctgc agtgtcgaaa
      841 atatcgtaga tcggccacgc atgctcgcgc tccggcatcg agcgtggcct gtttccggtc
      901 atttcaaatt cgaccatgtg ctgggcgacg agaaacaggc agttctcgaa caggccgatg
      961 cggatgtccg cgccggacgg tgcgccgccc cgcttctgat agagcgccgc gaggatggaa
     1021 attgcaccga acatgccgcc catgatgtcg ttggcggagg agccgacgcg ctgcggtttc
     1081 tccttcgttc cggtcatggc ggcgagcccc gacatcatct gcacgacctc gtcgtgcgcg
     1141 ggccgatggt cgtaggggcc ggacagaaag cccttgtgtc ccgcaacgat cagatgcgga
     1201 tgctgcttgc gcagctcctc cgctcccatt ccctgtttct caagctggcc gtcgcgaaaa
     1261 ttttccagga acacgtcggc cgtctcgagc agacgatgca tggtcttgcg gtcttcctcg
     1321 cgtgcaaagt cgagcacgac actgcgtttt ccccggttga acagcgggaa gaatgaggtt
     1381 cccatgccgc cgagagagcg ggtcttgtcg cctgcaggcg gctcgacctt gatcacttcg
     1441 gcgcccaact gcgcgaggat catgccgcac gtcgggccca tcaccatgtg ggtcatctcg
     1501 acgacccgca cgccctcaag cggcaacccg gatccctcca tgctgctctc ctttttttgt
     1561 caagtcacac taggctttca gcggccatct tgaaaatata atctttgcaa ccgcgcattc
     1621 ctgcttgcat aacgcgatgc gaaaacggcg cagccgcagt ggagcctggc ggtagtcgcc
     1681 gaggcaagcc gcccgagatc ggctgccgac acgggaggaa aggatgacga cagtcagcgt
     1741 cgcgactgga accgatgtcg cggccgcgtc cgacgggccc agcgaaatct cgcagcggct
     1801 ggagcgcctg ccggcgtcct cctatctctg gcggctggtg atcctgctct ccctcggcgg
     1861 ctgctttgaa atctacgatc tgttcctgac cggctatatc gcacccggct tgaaccgcag
     1921 cggcctgttg accacgacga cacaggcgtt tttcggcttt tccgggatcg gtgcttttgt
     1981 cgccgcgacc tttgccggct tgttcgccgg cacgttcttt ctgggcttcc tcgccgaccg
     2041 cttcggccgc cgcgcaatct tcacttttgc cctgctcggc tatagcgccg cctccgttat
     2101 catggcctgt cagaccacct ccggcggcct tctgttgtgg cgattcctgg caggtatcgg
     2161 catcggcgtc gaaatcatca cggtcgacgc ctacatcacc gaactcgtgc caagccggat
     2221 gcgtggtagc gccttcgcag tcaaccaggc cgtgatgttc atcgccgtgc ccatcgtggc
     2281 cttcctcgcc tggtggctgg tgccgctcac gccccatggc atcgatggat ggcgctgggt
     2341 cgtgctgatc ggcgcagccg ccagcatggt gatctggatc ctgcggctct acctgccgga
     2401 gagcccgctc tggctcgcac gccatggccg caacagcgag gcgatcgata tcgtacgcaa
     2461 gctcgaagcg gcgacaggcg ccgatcagcg tccgggacgc gcggcggaag cgcgcgcgtc
     2521 agtcgcgatc aagccagcgg gttttgccga cctgttgagg gcgccctatt tatcgctggt
     2581 cgtcctgttc atggtgttca atttctgcca ggcgtttggc ttctacggct ttgccaattg
     2641 ggtgccgacg ctattggtcg agaagggcat caccgtcacc aaaagcctcg aatattcgtt
     2701 catcgtcgct ttcgcctatc cgatcgcgcc gcttgtcgcc gcaagcttcg ccgaccgttt
     2761 cgagcgcaaa tggatcattt gcggcgcctg caccgcgatc atcatcttcg ggctctcgtt
     2821 tgcgcaactc gtgcagcccg cgctgctgat cgccttcggc gtgttgctga ccgctgccaa
     2881 catgacgatg tcctatgcct atcacgccta ccagaccgag gtattcccga ccgcgattcg
     2941 tgcgcgggcg gcggggctgg tctattcgat gagccgcatc agcgcgacct tttccggctt
     3001 catcgtcgcc ttcatgctca aggaaggcgg ggtcggcggc gtgttcggcc tgatcaccgc
     3061 cgccatgctg atcgtgatcg tcacacttgc ggcattcggt cccaacgtcc gcggcaaacc
     3121 gctcgatgct tgacgcattt gcagcccttg ccgaagcaag caggcgtatc taactctccg
     3181 tgggcaattg gttacgaggt aagacaatca tgatcgatgc cgccgtcaag gcgctttcgc
     3241 aaatcctgtc gccgccgatg cgttcgatcc tgtggcgatc gatcggactt gcgctggtgc
     3301 tgattacagt acttgcggtc ggcctgcagc gcctgctgag ctggtttgcg acgtatggcg
     3361 agggctgggc cgaaacgatg cttggtcccg gtttccacac gccgctgaac gtgctcgcct
     3421 ggatcgtgtc gatcgccgcc ggacttggcg tcgtgctcgg cgggatattc ctgatgccgg
     3481 cgatcacctc gctggtcgca agcttcttcg tcgacgatgt cgcggacctg gtcgagcgcg
     3541 agcattatcc ggccgagcgt cccggcgtcg cgctgccgct cgggcttgcc atgagcgagg
     3601 gcatccgcac cgctctgctg acgatcgtgg tctatctggt cgcgctgccc ttcgtgctgg
     3661 tcgccggcgc gggttttctc gtgttcttca ttgcgacggc gtggctgctc ggcaagcaat
     3721 atttcgagct cgcggccatg cgtttccgct cgcccgccga a
//
LOCUS       DATLJT010001605         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_845729,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001605 DATLJT010000000
VERSION     DATLJT010001605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845729"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VM910_36740"
     CDS             complement(<1..514)
                     /locus_tag="VM910_36740"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HVI68091.1"
                     /translation="MAQIVAGFGVPHTPIFPHFVKRDGPDCEIAKLFGAQKAQLAAER
                     PDLIVMFDTDHLNTFFLDGLPIFAVGIDSTFKAPNDEPRDVPNYIVPSMLDVAAYIRS
                     AVVAAGFDVGMTQNYSVDHSVIVPLHFLTPDMTVPVIPFFISGHVPPLPSAQRCHALG
                     RAVAHAIESWP"
     gene            complement(595..882)
                     /locus_tag="VM910_36745"
     CDS             complement(595..882)
                     /locus_tag="VM910_36745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68092.1"
                     /translation="MSIFAVNHLCREVLRDHAFRAAMKSDPAKALAPLDLSDDERRAL
                     LAGDVGTLYRMGVNAFLMNYLARFEVCGLNAKMYNQRMRAVKVDEIGQPVG"
     gene            complement(893..1789)
                     /locus_tag="VM910_36750"
     CDS             complement(893..1789)
                     /locus_tag="VM910_36750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVI68093.1"
                     /translation="MSQASPAPQEQDRYPYSAIVDRKPLRWPNGARVAVWVIPNIEHF
                     LFDRPSTSVTAVTASLVPDVLNYSWRDYGVRVGIWRMMEVMEKHGVKGTVALNSDVCR
                     RYPRIIEGGKQLGWEWMGHGNNNSTLINRQSEDEERELIKNVVDIIKASTGAQPRGWL
                     SPALSESHRTLDILAENGIQYVGNWVNDEQPYPMRVKSGSMISMPYSIEMNDIPVFLD
                     QGQAPETFGRMIRDQFDVLYEDGAKTGRVMSICLHPFLIGHPHRSKYFDQALAHIRSR
                     QEVWVTTGGEINDWYRKSYLNT"
     gene            complement(1810..2469)
                     /locus_tag="VM910_36755"
     CDS             complement(1810..2469)
                     /locus_tag="VM910_36755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVI68094.1"
                     /translation="MTERALQSDAEFFKQRGFGLKIGFGERPALLVIDMLKAFTNPQM
                     MLGANLDKEIEAIKPLLAVAHERDIPVIFSTVIYSDADLKDAGIWALKQKGVVTLKGG
                     SEGVKIDPRLDFRKTDSLLVKKYASCFFGTDLVSRLLSRRVDTLIIVGCTTSGCVRAT
                     AVDAVQTGLRPMVVREAVGDRSAAAHEQSLFDLNAKYADVVSLDETLQYLRTVGHNVN
                     A"
     gene            complement(2545..3486)
                     /locus_tag="VM910_36760"
     CDS             complement(2545..3486)
                     /locus_tag="VM910_36760"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HVI68095.1"
                     /translation="MSFLIPAGAGATPKGKKIAFLVTSPTHPFIATLSKTFVSRANSF
                     GMEVNTVSQHFDAALQAQQVDDAIARQFDMLAIIAGSEQAIVPALIRAKQAGVPVILV
                     NSPPRDGSEDLYLSFVGEDHVELGRITGVSVLQALKEGGRDGGQIALITGSLQEGVGP
                     RRVAGFRKAMKANPKAQIVAVEDAKWQTDLSERIAGQLFARFAAQGGLDVVYGMADNQ
                     AAAIVQAAKSAGIPLGVEKGKLIVVASNCLKQGIAGIKAGEQYSTGTQVPTRTGLKAA
                     EMIADHFNGKTLPKNEILPVETITKANVDKWEEACSY"
     gene            complement(4110..>4467)
                     /locus_tag="VM910_36765"
     CDS             complement(4110..>4467)
                     /locus_tag="VM910_36765"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68096.1"
                     /translation="IGIFAFSGLCAALSGVLLSYSLASASPAGLSDVIAPAAAGAILG
                     GISLGGGTGRPLGIAAGALTLAVLRSGLNAVGAPPFVNDIAMGAVLLTVAVLDGPDLA
                     RRLIVARRLARRSPAA"
BASE COUNT          821 a         1415 c         1418 g          813 t
ORIGIN      
        1 ccggccagga ttcgatggca tgggcgaccg cccggccaag ggcatggcag cgctgcgccg
       61 aagggagcgg tggcacgtgc ccgctgatga agaacggaat gaccggcact gtcatgtccg
      121 gcgtgaggaa gtgcaggggc acgatgacgg aatgatcgac cgagtagttc tgcgtcatgc
      181 cgacatcaaa cccggccgca acgacggccg agcgaatgta tgcggccacg tcaagcatgg
      241 acggcacgat gtaattcggg acgtctcgcg gctcgtcatt cggtgccttg aaggtgctgt
      301 cgatgccgac tgcgaagatc ggcaggccgt cgagaaagaa cgtgttcaga tgatcggtgt
      361 cgaacattac gatcaggtcc ggccgttccg cagcgagctg cgctttctgg gctccaaata
      421 gttttgcgat ctcgcagtcg ggaccgtcgc gcttcacgaa atggggaaag atcggagtgt
      481 gcggaacgcc gaagccggca acgatttgcg ccatggcacc aagcctaggt tggccctcgg
      541 ggcaatatta gacgcgcgca cgggatctgt gccaccggca gtttgagggc tgtgtcaccc
      601 taccggctgc ccgatctcgt cgaccttcac cgcgcgcatg cgctggttgt acattttcgc
      661 gttgagcccg caaacctcga agcgcgccag atagttcatg aggaacgcgt tgacgcccat
      721 tcgataaagg gtgccgacgt cgccggcgag cagcgcgcgc cgctcatcgt cgctgagatc
      781 gagtggggcg agcgcctttg ccggatcgga tttcatcgcc gcacggaaag catggtcgcg
      841 cagaacctca cgacagagat ggttgaccgc aaaaatggac atcgtcgcca cgctacgtgt
      901 tcagatagct cttccggtac cagtcgttga tctcgccgcc ggtagtcacc cagacctcct
      961 ggcgcgatct gatatgggcc agagcctgat cgaagtattt gctgcgatgc ggatgcccga
     1021 tcaggaacgg gtgcaggcag atcgacatga cccggccggt cttggcgcca tcttcataga
     1081 gcacatcgaa ctgatcgcgg atcatcctgc cgaacgtctc cggcgcctgg ccctggtcga
     1141 ggaacacggg aatgtcgttc atctcgatcg aatacggcat cgagatcatc gagccggact
     1201 tcacccgcat cggatacggc tgctcgtcat tgacccagtt tccgacatat tggatgccgt
     1261 tctcagccag aatgtcgagc gtgcggtggg attcgctcag cgcgggactc agccacccgc
     1321 gcggctgcgc cccggtactc gccttgatga tgtcgacgac gttcttgatc agctctcttt
     1381 cctcgtcctc ggactgccgg ttgatcagcg tggaattgtt gttgccgtgg cccatccatt
     1441 cccatcccag ctgcttgccg ccctcgatga tgcgaggata gcgccgacac acgtcggagt
     1501 tcagcgccac ggtacctttg acgccgtgct tctccatcac ctccatcatc cgccagatgc
     1561 cgacacgcac gccgtagtcg cgccaggaat agttgagcac gtcggggacc aggcttgcgg
     1621 tcaccgcggt gaccgacgtg gacggccggt cgaacagaaa gtgctcgata ttcgggatga
     1681 cccagaccgc cacgcgtgcg ccgttcggcc accgcagcgg cttgcggtcg acgatggccg
     1741 aataaggata acggtcttgc tcctgggggg ccggcgaagc ttgcgacata gctccgatcc
     1801 ttgtgcgctt tacgcgttca cgttgtggcc gacggtgcgc aggtactgca gcgtctcgtc
     1861 gagcgagacc acgtcggcgt acttggcatt gagatcgaac aggctctgct cgtgggcggc
     1921 cgccgatcgg tcaccgaccg cctcgcgcac caccatcggc cgcagcccag tctggacggc
     1981 gtcgaccgcg gtggcgcgca cgcagccgct ggtcgtgcag ccgacgatga tcaaggtatc
     2041 gacgcgccgg ctcaagagcc gcgacaccag atcggtgccg aagaagcagg aggcgtattt
     2101 cttcaccagc aggctgtcgg tcttgcggaa atcgagccgc gggtcgatct tcacgccctc
     2161 gctgccgccc ttcagcgtca ccacgccctt ctgcttgagt gcccagatgc cggcatcctt
     2221 gaggtcggcg tcgctgtaga tcaccgtcga gaagatcacc gggatatccc gctcgtgcgc
     2281 aacggccagc agcggcttga tcgcttcgat ctccttgtcg agattagcgc cgagcatcat
     2341 ctgcggattg gtgaaggcct tgagcatgtc gatgaccagc agcgctggcc gctcgccgaa
     2401 gccgatcttc agcccaaacc cgcgctgctt gaagaattcg gcgtcgctct gcaatgcccg
     2461 ctcggtcatg cttcggttgc ccctctacgc gccggacgtc gatggtccgc gcgcggccgg
     2521 ccacacggac aggcttctgt cgcctcagta ggagcaggcc tcctcccact tgtcgacatt
     2581 ggccttggtg attgtctcga ccggcaggat ctcgttcttt gggagcgtct tgccgttgaa
     2641 gtgatcggcg atcatctccg ccgccttgag cccggtgcgg gtcgggacct gcgtgcccgt
     2701 gctgtactgc tcgccggcct tgatcccggc aatgccctgc ttgaggcagt tactggcgac
     2761 gacgatcagt ttgcccttct cgacgccgag cggaatgccc gccgatttcg cggcctgcac
     2821 gatggcggcg gcctggttgt cggccatgcc gtagacaacg tcgagcccgc cctgggccgc
     2881 aaatcgcgcg aacaactggc cggcgatgcg ctcgctgagg tcggtctgcc acttggcgtc
     2941 ctcgacggcg acgatttgag ccttcggatt ggccttcatg gccttgcgga agccggcgac
     3001 ccggcgcggg ccgacaccct cctgcagcga gccggtgatg agcgcgatct ggccgccgtc
     3061 gcggccgccc tccttgagcg cctgcaatac cgatacccct gtgatgcggc cgagctcgac
     3121 gtggtcctcg ccgacgaagc tgaggtagag gtcctcggag ccgtcgcggg gcggggagtt
     3181 gaccaggatc accggcacgc cggcctgctt ggcgcggatc agcgccggca cgatagcctg
     3241 ctcggagccg gcgatgatgg ccagcatgtc gaactggcgg gcgatggcgt cgtcgacctg
     3301 ctgcgcttgc aaggctgcgt cgaaatgctg gctgaccgta ttgacctcca tgccgaatga
     3361 gtttgcgcgg gagacaaagg tcttgctcag ggtggcgatg aacggatgcg tcggggacgt
     3421 gacgagaaac gcgatcttct tgcctttcgg agtggcgccc gctccggcag ggatcaggaa
     3481 cgacaagcta agtgcagtga tcagcgcaac cgctagcttc accatatctg cctccctcga
     3541 agcacgacct cgccattcat ttgtaggacg cgcgccaagg cggcgcaacc gcccagccgg
     3601 gcgagtaatg cgctcctagc gcatcgcgag cagtgtatgc gcgagtatga gagtggttct
     3661 cgaccttctt tgagaacgcg ttagcgagcc gaataaagcg ctgcgcaagt gggcggcaat
     3721 cctcggcact cgccaacaaa atccgcaacg tctggggcat cttgcgcggc ggtcgcaact
     3781 tcgaggtcag aacgctcagg cccgcgctcc aagctggcgt gagtctaccg acatcgctag
     3841 aagcgtcatg tgagcccggc aatcactatt catcaccgag gtctgcgaga gaaatagaag
     3901 agatggagaa aaggccttcc agcgcatccg agaactgatg acccgaatag cccttcgagg
     3961 catggccggt aataaggttg gacgcgcgcc catttccatc atagtccgga gcatgagctc
     4021 caacacgagg cctgatacat tgatgcaaga ccgtactctc caggctcatc gttttctctt
     4081 gaacgcacgg cgggtccata catcccgggt caagccgccg gcgagcggcg tgccaatcgc
     4141 cgcgccacga tcagccggcg cgccaggtcg ggaccatcca gaaccgccac cgtcagcagc
     4201 acggcgccca tagcgatgtc gttgacgaag gggggcgcgc ccacagcatt gagcccggag
     4261 cggagcaccg cgagcgtcag cgcgccggcc gcgatgccga gcggccgccc ggtgccgccg
     4321 cccagcgaga tgccgccgag gatcgcgccc gccgcagccg gcgcgatgac atccgacagt
     4381 cccgccggtg acgccgacgc aaggctgtag ctgagcagaa ccccggacag cgcggcgcac
     4441 agcccggaga acgcgaagat gccgatg
//
LOCUS       DATLJT010001606         5044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2227242,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001606 DATLJT010000000
VERSION     DATLJT010001606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227242"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VM910_36770"
     CDS             <1..290
                     /locus_tag="VM910_36770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68097.1"
                     /translation="QVVRQRWVVIDEYGGTIPPLLITMVILWLAIIFASFGYRAPRNT
                     SVTASFFLSAARFLLGTFFAAERTVPNSYPKLDLLWDNAIAGPWGTPRKNV"
     gene            468..635
                     /locus_tag="VM910_36775"
     CDS             468..635
                     /locus_tag="VM910_36775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68098.1"
                     /translation="MPASLINDPEHWRKRADEARSLADDMKDEISKQMTLQIADDYEH
                     LAKRAEQGAKL"
     gene            1011..2279
                     /locus_tag="VM910_36780"
     CDS             1011..2279
                     /locus_tag="VM910_36780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVI68099.1"
                     /translation="MTETITPASLYVAANGNDNNNGKSPSAPFQTIAKINSLVLAHGS
                     SVLFNGGDTFAGNLLPNIDGNGDPNNPVIIGSYGTGQATLVAGVGGETGVVNIEQMSG
                     VTVRNLIIRGATVLTNMPRGGIRIGNNSATRRTGITVDNCDIGGITYFEATQGGNPSV
                     QGTNGFGIYLAGYPGAGFDVTITNNKVHGLNGPTSHDDVGIGGFGNGSNIGVTLNNNL
                     VYNIGGGPPGLASGVGYPPMGDGIAIDGSSGGLVQNCTVHDCGANYRNPAGGPAGFLS
                     ANSRNVKFYRCLAYNIAPSDFSLTQVDFVGFDLDNDSNGCTIDSCYAHDCYNSGFMLF
                     SNGDAGWNNNTITNCLAENNCRGGLPGFGEITINLPGASDPTITVQKNTTFNNRVYTG
                     QQFHNDNQGAVGIGSQAAACFKAPFRRTSS"
     gene            2276..2575
                     /locus_tag="VM910_36785"
     CDS             2276..2575
                     /locus_tag="VM910_36785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68100.1"
                     /translation="MTNVDIYGESTPLNARTNTSTFTPAVEISNNDWWQQSGGLDIWW
                     ANTQYFTLANWQTASDKGANTQLGNPNFSAPGPAGHTQMTFPGWGATPQSSYGST"
     gene            4452..>5044
                     /locus_tag="VM910_36790"
     CDS             4452..>5044
                     /locus_tag="VM910_36790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI68101.1"
                     /translation="MALLPLFRSREREAELEHRVYALQAALAQCKGVARTWWSMRVRF
                     TIAVALVAMSAGFALGVYRHSIKQAFVNSAVAVGLASPADGAAEAETAFQKTDYAKAL
                     RLARPLAEAGDPRAEAVLGSAFYRGRGVPQNDSEAAKWFRLAADKGNAAARFTLGVMY
                     GEGRGVPQDYAEAARWYRRAAEQGDAQAQYNLGLAYAR"
BASE COUNT          997 a         1573 c         1535 g          939 t
ORIGIN      
        1 gacaggtcgt acggcagcgt tgggtcgtga tagacgaata cggcgggacg atcccaccgc
       61 tgctgatcac catggtgatc ctctggctgg ccatcatctt cgccagtttc ggatatcggg
      121 ctccgcgcaa caccagcgtg acagcttcgt tcttcctgtc agccgcaagg ttccttctgg
      181 gtacattttt cgcggcagaa cgcacggtac cgaatagcta ccctaagctt gatctgctgt
      241 gggacaatgc gattgcggga ccgtggggaa ctccccgcaa aaatgtctga cagccagtcc
      301 cggcccgtca ccccgccatg cggcgggtcg ggacgcttac agaatgcgcg agctcacgat
      361 tcccgtcaac gaactctcta cctcgggtag tggtcaggtt gattaatggg gtgaaagcgt
      421 ggaggcgaag tgcgagcgac ctgcggcggt cgcgtgagag gaactttgtg ccggcctccc
      481 ttattaacga ccctgagcac tggcgcaaac gcgccgacga agcgcgcagt cttgccgatg
      541 atatgaagga cgaaatatcc aagcagatga cgcttcaaat cgcggacgat tacgagcacc
      601 tcgcaaagcg tgccgaacag ggggcgaagc tttagtcgtc gggcacgacc agcctggcgc
      661 cggctgccct cgaacggcgt atcattcgcc aaaacgagaa ccccgcccga gtccgagtta
      721 gggcgtggtc ttcgtgaacg ctcagcgact aacgcaagat cagtcgcggc gtcttatcct
      781 gcgaacactc ctgttatcgg tttgccggct atgcgccgat cgcgctcatc tcccgctcgc
      841 cgctcgttat cgacgaaagt cagatgctca gatgtgggac cagcattatg acgagccgtc
      901 gtaaatttat agccgtgctt agcgcgggag tgtcgtgggc agcgatagcg cgagcggcga
      961 cttttaccgg cagcggcgtc gatgaccaag gcaatacgat tacgtttacc ttgacggaga
     1021 cgatcacgcc tgcatcgctt tacgtcgcag ccaacggcaa tgacaacaac aacggcaaat
     1081 cgccgagcgc gccgtttcag acgattgcca agatcaactc gctggtgctg gcacacggta
     1141 gctcggtcct gttcaatggc ggcgatacct tcgctggcaa ccttttgccc aacatcgacg
     1201 gcaacggcga cccgaacaat cccgtcatca tcggcagtta cggcaccgga caagccactt
     1261 tggttgcggg tgtgggcggt gaaaccggcg tcgtgaatat cgagcagatg tcgggcgtca
     1321 ccgtgcgcaa tttgattatt cgtggcgcga ccgttctcac caacatgcca cgcggcggca
     1381 tcaggatcgg caacaacagt gcgacgcgcc gcactggcat caccgttgac aactgcgaca
     1441 tcggcggcat cacctatttc gaggcgacgc aaggcgggaa tccgagcgta caaggcacca
     1501 acgggttcgg catctatctc gcaggttatc ccggtgccgg cttcgatgtg acaatcacga
     1561 acaataaagt ccacggcctc aacggcccga cctcgcatga tgacgtgggc atcggcggtt
     1621 tcggtaacgg ctctaacatc ggcgtcaccc tcaacaacaa tttggtttac aacatcggcg
     1681 gcgggccgcc cggcctcgca tccggtgtcg gctatccgcc gatgggcgat ggcatcgcta
     1741 tcgacgggtc cagtggcggg ctggtgcaga attgcaccgt gcatgattgc ggagccaact
     1801 atcgcaatcc agcgggcggc cctgccgggt ttctctctgc caacagccgc aacgtgaagt
     1861 tctatcgctg cttggcgtat aacatcgcgc cctccgattt ctcgctcacg caagttgatt
     1921 tcgttggctt cgacctcgac aacgacagca atggctgcac gattgactcg tgctacgcgc
     1981 atgattgcta caacagcggc ttcatgctgt tctcaaacgg cgacgccggc tggaacaaca
     2041 acacgatcac taactgcctc gcggagaata attgccgtgg cggcctgcca gggttcggtg
     2101 agatcaccat caatctgcca ggcgcatccg accccaccat cactgtccaa aagaacacga
     2161 cattcaacaa tcgtgtctac acgggtcagc aattccacaa cgacaaccag ggcgcggtcg
     2221 gcatcgggtc acaggcggcg gcgtgtttca aggcaccatt tcgaagaaca tcatcgtgac
     2281 gaatgtggac atctacggtg agtccacgcc gctcaacgcg cgcaccaaca cgtcgacgtt
     2341 cacgcccgca gtagagatct cgaacaacga ctggtggcag caaagcggtg gcttggacat
     2401 ctggtgggct aacacccagt atttcacgct ggcgaactgg cagaccgcga gcgacaaggg
     2461 agcaaacacg cagctcggca accccaattt cagcgcgccg ggaccggcag gacacacgca
     2521 aatgacgttt ccgggctggg gcgcaacacc acaatcgagt tacggcagca cgtaaatgcc
     2581 caccgcagcc ctgcgcagcg acagccgcta ataatagtaa tatccgaggc gatgtgctct
     2641 tcggtgaacc cttcgattaa cgcctgcaac gctcgtcgca gttagcggtc gccctacccg
     2701 ggcatcagca ctgtcaaggg agagcgccac attcttttgc gaccattgaa gcgagaaggg
     2761 cgccgcactc aataacgcgg ctccaacgat aatcccaagc acacagagct tgttcatgat
     2821 ggctctccat gatgatgtcg cgcccctatt gaggtaacgc ggcgcgtgcg catagcgttc
     2881 ccctcaaacc cccacgaccg gaataccgtc gacataaaag aaagccccag acgtgaagcc
     2941 ggggcttccc gtcggcgagg acgtcgtagt cgtcgtcatt ccattcgcct gatgggcggc
     3001 tcgccgccgc gcgtttaaga atcagttggg ccatcggaga gagtgagcga aatctttgat
     3061 gatcggccat gcggcaatgc acgccgcaac tgccgccacg atggtccagc acagtaccca
     3121 gcgataccgg cgcgtttgct cttgttcgat tcgcgcttct tggtcgcgaa gccaccgctc
     3181 gacgtaaccc ttggtgatgt tttgttccgt gcccacgctg atgctcgcgg ttgacccgcc
     3241 ggggctttgc acaagacggg cgcgaacaac atggatgcca gcggtttcaa gcgcgcgcag
     3301 ccatcgtccc tccatcacgg ggtctgacgg acggtaatct tcttcgggag ccattcgggc
     3361 gcgctgtccc cagattggtg cggctgccgg acttgcaccg gcacctcccc ggccccaatg
     3421 tcgccgttgg tcaaacgaga cgactcggtc gaaagcgggg gcgtccttct tagacgacgc
     3481 cgcgccgtcc ctcctacgac gcgcgcgcga ttcgtgggga aattaccccc aacaatttcc
     3541 gacacgtgga taagttgccc cgatttggcg cgggcagatt gctcgccggg acactattgg
     3601 gacactatcg ccgtccatcg ttgaaatcgt tatgttattt cggcccgtat cttgctgcgt
     3661 cataggacgc aaggtgcctc actaggcgcc tggcctgccg cgtcatgtgc aggccccacg
     3721 ggtgccgctg cacccgaacg tttctcggcg ctccgcccac ccctcgattc gggtgagtga
     3781 agcaacgatg caaaaccccg ggcgcaaacg cgccgcggga acgagtagat ctgggctgtt
     3841 tgacatcgtg agatgggaat gaccgaaaac ggttcggcga cgagccgcgg actcggccgt
     3901 cctcatcctg aggagcgcgc ctgctggaag atcccgcaaa gcccgaacgg gcgtgcgcgc
     3961 gtctcgaagg atgagggcgg cagttacccc ctcatgcttc gagacggcgg ccatagcgcg
     4021 ttgaagacgc gcgtaaacgc gctttaggcc gcctcctcag catgagggag gagagcgcgg
     4081 aggatggggc cctcatgcct cgagacgcca cgctgcgcgc ggctcctcgg catgaggagt
     4141 tcacgatcgc gcgagcggga cgtcgtgcaa agccagggtt gtggcttttc cctgttttcg
     4201 tcctgttccg tcctgttatc gacggcagat cgccgcgatc cgagcgacgc cgccggcgcg
     4261 gatttctcgt tttgatcccg ccgcttacgg gaaacgagaa acgcagggtt gtggccgggc
     4321 cgcctgtttt tctcctgtta tttacaggga aatacagggc cgcgggcgtc gccgttgcac
     4381 gttgccgatg ccgcagtgga tcactacctc acgcggtgtc ggcgggggcg cggttgaggg
     4441 actgattgcg catggccttg ttgccactgt ttcgaagccg ggaacgggag gccgagctcg
     4501 agcatcgcgt ctacgcgctg caggcggcgc tcgcccaatg caagggcgtc gcccggacgt
     4561 ggtggagcat gcgcgtgcgc ttcaccatcg ccgtcgcgct ggtcgcgatg tcggcgggct
     4621 ttgcgctcgg cgtttaccgg cattcgatca agcaggcctt cgtcaacagc gccgtggccg
     4681 tggggctcgc gagcccggcg gacggcgcag ccgaggccga gaccgcgttc cagaagaccg
     4741 actacgccaa ggcgctgcgg ctcgcgcgcc cgctcgccga ggccggcgat ccccgcgccg
     4801 aggcggtgct gggctccgcc ttttaccgcg gccgcggcgt gccgcagaac gacagcgaag
     4861 cggcgaagtg gtttcggctt gccgccgaca agggcaacgc cgccgcgcgc ttcacgctcg
     4921 gggtgatgta cggggaaggc cgcggcgtgc cgcaggatta cgccgaggcg gcgcgctggt
     4981 atcgccgcgc cgccgagcag ggcgacgccc aggcgcagta caatctcggc ctcgcctacg
     5041 cccg
//
LOCUS       DATLJT010001607         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2457186,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001607 DATLJT010000000
VERSION     DATLJT010001607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457186"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VM910_36795"
     CDS             complement(<1..470)
                     /locus_tag="VM910_36795"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HVI68102.1"
                     /translation="MPLLEATRGGLLKVGTSTLTGILNRRGLRNMFMQDVNPLRVTQP
                     RMVGLAFTMRFIPSREDKAGPNAPNGGRTQVQAMEECPPGYVLVIDSRGDPRAASAGD
                     LYVGRLKARGCAGIVTDGGLRDSEGILKTGLPGYLRRPSSPPSPIVHQPVDLNLP"
     gene            complement(457..>2670)
                     /locus_tag="VM910_36800"
     CDS             complement(457..>2670)
                     /locus_tag="VM910_36800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI68103.1"
                     /translation="IVSIDVQAARAAPGVLAVYTGADYKADGLSMPKAVMPRKKADGS
                     PMFAPQRPALVIDRVRYVGDPVAMVIAETLMEAKDAAELIAVVYDELPSVTSTDEAAR
                     PDAARVWDENPDNISHTVQHGNKAATDAAFAQAAHVARQRYVITRVHAQYMEPRGAIG
                     SYDAFENRYTLYADVNYPHRVRNMLATSVFNVPESAVRVVCHDVGGGFGAKGWQYVEH
                     RLTLWAARKLGRPVKWTCERSEVILADEHARDNVGDIELALDKDGKFIGVRMHMLANL
                     GAYIGSDRQLLTPFGQIVTLTGVYNIPAAHVTIDAVLSNTNPTAPYRGAGRPEACYLI
                     ENIIEVAAREMNIDRIDLRQRNLIKPDAMPYRAALGPLYDCGDFPKNMAMALEHSDYA
                     GFEARRAASATAGKLRGIALVNAIEQAAGPVPEYAEVRFQPSGSAMILLGTKNHGQGH
                     ETAFKQILFDKLGIDPNEVQFIDGDTDRVAFGMGSNGSRSMVTGGTALVLAANKIIEK
                     GKLIAANLLEVGADDVTFADGRFSVVGTDRGLTLKQVVAASFLPARLPKGLPPGLIES
                     ATYSPEQSTYPNGCHVCEVEVDPETGAVELKSYLVVDDVGTVINPLTLAGQIHGGVAQ
                     GVGQILMEQVAYQPGSGQLLTASFMDYAMPRADTMCNIAIRSNPVPTATNLLGAKGAG
                     EAGTVGAMPAVMIALLNALAPVGVRTLEMPATSDRVWRAIQAARIAATGGTNATA"
BASE COUNT          467 a          882 c          820 g          501 t
ORIGIN      
        1 ggcagattga gatcgaccgg ctggtgcaca atcgggctcg gcggcgagct cggccgccgc
       61 aagtagccgg gcaggcccgt tttcaaaatg ccctccgaat cccgcaggcc gccgtcggtg
      121 acgatgccgg cgcagccgcg cgctttcagc ctgccgacat agagatcgcc ggcggacgca
      181 gcccttggat cgccacgcga gtcgataacg aggacatatc caggtgggca ttcctccatc
      241 gcctgcactt gtgtcctgcc gccattgggt gcgtttggac cggccttatc ctcgcgcgac
      301 gggataaagc gcatggtgaa cgcgagccca accatgcgcg gctgggttac gcgcaacgga
      361 ttgacgtcct gcatgaacat gttgcgcagc ccgcgccggt tgaggattcc ggtgagtgtc
      421 gacgtaccaa ctttgagcag accccctctc gttgcctcaa gcagtggcat tggttcctcc
      481 cgtcgcagca atgcgtgcag cctggatcgc tcgccagacc cggtcgctcg tggccggcat
      541 ctcgagagtg cgcacgccga ctggcgcgag cgcattcaga agcgcaatca tcacggccgg
      601 catcgcaccc accgtgccgg cctcgcccgc acccttggcg cccaacagat tggtcgcggt
      661 cggcacgggg ttgctcctga tcgcgatgtt gcacatcgta tccgcgcgcg gcatcgcgta
      721 gtccatgaag cttgcagtca ggagctggcc gcttcccggc tgataggcga cctgttccat
      781 caggatctgg ccgacgccct gtgcgacgcc gccgtgaatt tgcccggcca gcgtcaatgg
      841 attgatcacg gtgccgacgt cgtcgaccac gagatacgat ttcaactcaa ccgcgccggt
      901 ctcgggatcg acctcgactt cacagacgtg gcagccattc ggataggtcg actgctccgg
      961 cgagtaggtc gcgctttcga tcagtcccgg cggcaatccc tttggcagcc gcgccggcag
     1021 gaaagaggcc gcgacaacct gcttcagcgt gaggccgcga tcggtgccga caacgctgaa
     1081 cctgccgtcc gcgaaggtta catcgtccgc cccgacttcc agaaggtttg cagcaatcag
     1141 cttacccttc tcgataatct tgttggcggc gagcaccagc gcggtgccgc cggtcaccat
     1201 tgagcgcgag ccgttcgagc ccatgccgaa cgcaacgcgg tcggtgtcgc catcaatgaa
     1261 ctgtacctcg ttcggatcga tcccaagctt atcgaaaagg atctgcttga acgcggtctc
     1321 gtggccctgt ccgtggttct ttgtaccgag caaaatcatc gcgctgccac tcggctggaa
     1381 gcgcacctcg gcatattccg gcaccggccc tgccgcctgc tcgatagcgt tgaccagcgc
     1441 gatgccgcgc agcttgccgg ccgttgccga agcggcccgc cgcgcctcga acccagcgta
     1501 gtcggagtgt tcgagcgcca tcgccatatt ctttggaaaa tctccgcagt cgtaaagagg
     1561 cccgagcgca gcgcgatacg gcatcgcgtc gggcttgatc aggttacgct ggcgcaagtc
     1621 gattcgatcg atgttcattt cgcgtgccgc gacctcgatg atattctcga tcagatagca
     1681 ggcttccggc ctgcccgcgc cccggtaggg agcggtcgga ttggtgttcg acagaaccgc
     1741 atcgatcgtc acatgcgctg cgggaatgtt gtagacgccc gtcagcgtaa cgatttgccc
     1801 aaacggcgtc agcagctggc gatcggagcc gatataggcg ccgagattgg cgagcatgtg
     1861 catgcgcacg ccgataaact tgccgtcctt gtccagggcg agctcgatgt cgccgacgtt
     1921 gtcgcgggca tgctcgtcgg cgaggatcac ttcggatcgt tcgcacgtcc acttcaccgg
     1981 acgcccaagc ttgcgcgccg cccacaacgt gaggcggtgc tcaacatact gccagccctt
     2041 ggcgccgaaa ccgccgccga cgtcgtggca aacaactcgc accgcgctct cgggaacgtt
     2101 gaacacggaa gtcgcgagca tgttgcgcac acggtgcgga tagttcacgt cggcataaag
     2161 cgtgtagcgg ttctcgaagg cgtcatatga cccgatcgcg ccgcgcggct ccatgtactg
     2221 cgcgtgcacg cgggtgatga cgtagcgttg ccgcgccaca tgcgcggcct gcgcgaatgc
     2281 ggcgtcggtc gcggccttgt tgccgtgctg aaccgtatgc gagatgttat cggggttctc
     2341 gtcccacacc cgcgctgcat cgggtcgagc cgcttcgtcg gtcgacgtca ccgacggcag
     2401 ctcgtcatag acgaccgcga tcagctcggc cgcgtccttc gcctccatca aggtctcggc
     2461 gatcaccatg gcgacgggat cgccgacata gcgcacccga tcgattacca gcgccggtcg
     2521 ctgtggcgcg aacatcggcg atccatcagc ctttttgcgc ggcatcaccg ccttcggcat
     2581 gctcaagccg tcggccttgt aatccgcacc ggtgtagacg gcgagcacgc ccggagccgc
     2641 gcgcgccgcc tgcacatcga tcgatacgat
//
LOCUS       DATLJT010001608         1628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3838116,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001608 DATLJT010000000
VERSION     DATLJT010001608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838116"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VM910_36805"
     CDS             complement(<1..777)
                     /locus_tag="VM910_36805"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI68104.1"
                     /translation="MNILVFGAGVVGSLYAGRLAAAGQDVALLARGARLAQLRREGLT
                     LVDEASGEETSPRLRIVEEYLPDDIYDVVIVATRADQIAEVLPVLAANRRVSCVIFLQ
                     NCASGPANLIAALGRERVVLGFPGAGGAREDARVQYRLIPQQKTMLGEVSGCVTPRLH
                     RIASVLQAAGFPVAFSRRMDGWLKTHAVLVTAIAGAIYLAEGTCANVASGADGVPRLV
                     RAVRQGFHALRTAGVFIEPRKLAILFLWLPFSVPVTYWRRY"
     gene            complement(1277..1551)
                     /locus_tag="VM910_36810"
                     /pseudo
     CDS             complement(1277..1551)
                     /locus_tag="VM910_36810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196150.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
BASE COUNT          320 a          520 c          485 g          303 t
ORIGIN      
        1 gtagcgccgc cagtaggtga cggggaccga aaatggtaac cagaggaaca agatggcaag
       61 cttgcgcggc tcgatgaaca cgcccgcggt gcgcagagcg tggaagccct gtcgcactgc
      121 acggaccaag cgcggcaccc cgtccgctcc cgaggcgacg ttcgcgcaag tgccctctgc
      181 aagataaatc gccccagcaa tcgcggtgac caacacggca tgggttttca gccacccgtc
      241 catgcggcgg ctaaacgcca ccgggaaccc cgccgcctga agaactgaag cgatccgatg
      301 aaggcgtggg gtgacacacc cgctgacttc gcctaacatt gttttctgct gcggaattag
      361 ccggtattgc acccgtgcgt cctcacgcgc accgcctgca ccagggaacc ccaaaaccac
      421 gcgttcgcgt cctaacgccg ctatcaggtt ggccggacct gatgcgcagt tctgcaggaa
      481 tatgacgcaa gaaacgcgcc ggttcgcggc aagcacgggc aggacttccg ctatttgatc
      541 cgcacgcgtc gcgacgatga ccacgtcgta gatgtcgtcc ggcaggtact cttctacgat
      601 ccgcagccgc gggcttgttt cctcgcccga agcctcgtcg acgagcgtca atccctcccg
      661 ccgaagttgc gctagtcggg ctccgcgggc cagcagggcg acgtcctgcc ccgctgccgc
      721 gaggcggcca gcataaagac tgccaacaac gcctgcaccg aacactagaa tgttcacggc
      781 tcacttataa cgacgaaaga gaaagcgcga catgttggcg tttcaattcc tcatcatccg
      841 ggcggcatat tttcccgcgc gatgaagatc gacaacagcc ttgccggtta cgtcagacgc
      901 acagatgacc gctagtcccc gaagaccgct tacggggttg agctgccgct gttccaggtg
      961 ccgtgaagcg gcgagttgcc cccattgcgg ccgttgcgta tagccagtcc cagggtctca
     1021 gcagggtggg gttcagtcct tggctcacac acgagccaat ggcgaggtag cgccgacgcg
     1081 gttattggcc aatctcgccg gcggctcaga tcgaccggcg cagcgtgccc tcctcatcca
     1141 cgcgcaacag cgcccatgca ctcgtctggt ctggccgagg cggatcgaaa ctggtcaact
     1201 ggtccccagc gcggctgcgt gacggcgacg agggtccgca tgatctcttg tagccccggg
     1261 catgcggccg cgttcgttac cggtcgatgt cgcccatgcg gaggtatttg acttcgagga
     1321 attcctcgat gccgtgtttc gaaccttcgc ggccgatgcc gctctccttc atgccgccga
     1381 acggcgcggt ctcgatcgag atgatgcctt cgttgatgcc gacgatgccg tattcgagcg
     1441 cctcggcgac gcgccagatg tggccgaata ggcgagcgtc ggtcttgaag cggtagagcg
     1501 gcgcgacggg tccgaaaagt tttcctcctt cgtaatcacc atgtcgctcg tcacatcagc
     1561 aatttgccga tttcgatcga gccggttgaa cgggcagaaa ggtgtatcta catataacct
     1621 atgagatc
//
LOCUS       DATLJT010001609         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2689209,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001609 DATLJT010000000
VERSION     DATLJT010001609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2689209"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /gene="dnaE"
                     /locus_tag="VM910_36815"
     CDS             complement(<1..1856)
                     /gene="dnaE"
                     /locus_tag="VM910_36815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVI68105.1"
                     /translation="MPAEPAFVHLHVHSSYSLLEGALTIARLAELAKNDRQPALALTD
                     TDNMFGALEFSEKLASFGIQPIVGCAIAIDFGDIVNRSTEHSWPRLVLLATDESGYRS
                     LMRLCSRAYLETAAQERPHIKISWLDETIDGLIALSGGPNGPLDGVLVAGQHPVALSR
                     CQELQRLFGDRLYIELQRHGMAGERLAEPQLIDLAYSRGLPLVATNEPFFATRDDYEA
                     HDALLCIAEGKLVADGERRQLTPEHRFKTRAEMTALFADLPEALAATIEIAQRCVFRP
                     RTQAPILPRFAAGRSERAAANENGERDQQVQEAAEAEELRRAARSGLERRLQRYGTAP
                     GHSVEDYNERLEFELGVIVNMHYAGYFLIVADFIQWAKAQDIPVGPGRGSGAGSLVAY
                     ALTITDLDPIRFGLLFERFLNPERISMPDFDVDFCQDRRDEVIRYVQQKYGRDQVAQI
                     ITFGTLQARGVLRDVGRVLQMPYGQVDRLCKLVPQNPAHPISLGRAIEDEPRLQAARD
                     GEPIVRRAFDIAQKLEGLTRHASTHAAGIVIGDRPLTELVPLYRDPKSLMPVTQYNMK
                     WVEPAGLVKFDFLGLKTLTVLDRAVKLVRRRGIEIDLSNLPLDDPKTYEMLAR"
     gene            2488..>2735
                     /locus_tag="VM910_36820"
     CDS             2488..>2735
                     /locus_tag="VM910_36820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68106.1"
                     /translation="MAVVLIVEDEDQVRVLAESYLEEQGHQVLSAGTPAGALALLHQL
                     PAVDLLFTDLDLKGDTHAGIELAKAAVKLRPQLHVLYTT"
BASE COUNT          536 a          832 c          850 g          517 t
ORIGIN      
        1 cgcgcgagca tctcataggt tttggggtca tccagcggca aattggacag atcgatctcg
       61 atgccgcgcc ggcgcaccaa tttgacggcg cgatcgagca ctgtgagcgt cttcaagccc
      121 agaaaatcga acttcaccag acctgccggc tccacccatt tcatattgta ttgcgttact
      181 ggcatcagcg atttcggatc gcggtagagc ggcaccaatt cggtcagcgg ccggtccccg
      241 atcacaatgc cggcggcgtg ggtcgaggca tggcgggtga gcccctcgag tttttgtgcg
      301 atatcgaaag cgcgccgcac gatcggctcg ccatcgcgcg ccgcctgcag ccgcggctcg
      361 tcctcgatgg cgcggccgag cgaaatcgga tgggccggat tctgcggcac cagtttgcac
      421 aagcgatcga cctgaccgta gggcatctgc aacacccggc cgacgtcgcg caaaacaccg
      481 cgcgcttgca gcgtgccgaa ggtaatgatc tgcgcgacct ggtcgcgtcc atatttctgt
      541 tgcacgtagc gaatgacttc gtcgcggcgg tcctggcaga agtcgacgtc gaaatccggc
      601 atcgagatac gttccggatt aaggaagcgc tcgaacagaa gcccgaaacg gatcggatcg
      661 agatcggtga tagtcagcgc ataagcgact agcgagccgg cgcccgagcc gcgcccggga
      721 ccgaccggga tgtcttgcgc ctttgcccat tgaatgaaat cggcgacgat caggaaatag
      781 ccggcgtaat gcatgttgac gatgacgccg agctcgaatt cgagccgctc gttgtaatcc
      841 tcgacgctgt ggcctggcgc ggtgccgtag cgctgcaagc gccgctccaa tcctgagcgc
      901 gcggcgcggc gcaactcttc cgcctcggcg gcttcttgca cctgttggtc ccgctcgccg
      961 ttctcgttcg ctgccgcgcg ctcgcttcgg ccggcggcga agcgcggcag gatgggcgcc
     1021 tgcgtgcgcg ggcggaacac acagcgctgt gcgatctcga ttgtggcggc gagcgcttcc
     1081 ggcagatcgg caaacaaagc ggtcatctcg gcgcgggtct tgaagcgatg ctcgggcgtg
     1141 agctgacggc gctcgccgtc ggcgacgagc ttgccctcgg caatgcaaag gagcgcgtcg
     1201 tgggcctcgt aatcgtcgcg ggtggcgaag aagggttcgt tggtggcgac gagcggcagt
     1261 ccgcggctat aggcgagatc gatcagttgg ggctcggcga gccgctcgcc cgccatgccg
     1321 tggcgctgca attcgatgta gaggcgatcg ccaaacagcc gttgcagctc ctgacaccgc
     1381 gacagcgcga ctggatgctg tccggcgacc agcacgccgt cgagcggacc attggggccg
     1441 ccggacagcg cgatcaagcc gtcaatcgtc tcgtcgagcc aggatatttt gatgtgcggc
     1501 cgttcctgcg ccgcggtctc aaggtaggcg cgggagcaca gccgcatcag gctgcggtag
     1561 ccgctttcgt cggtcgcgag caaaaccagg cggggccaac tatgttcggt gctacggttt
     1621 acgatatcac caaaatcgat cgcgatcgcg catccgacga tcggctgaat tccgaagctc
     1681 gccagctttt ccgagaattc cagcgcgccg aacatattgt cggtatcggt caaggcgagc
     1741 gctggctgcc gatcgttctt ggcaagttcg gccagccgcg caatcgtcaa tgcgccttcg
     1801 agcagcgagt aggacgaatg aacgtgcaaa tgcacaaaag cgggctcagc tggcatcaaa
     1861 tggcgtccgt cgggtcaagc ccctgctaac gagtggccca tgattagaat ggcgatcgcg
     1921 attctcgccc gatcaaccat gcgggattgg aaacgacact gtccatcgtc ccgcacgcga
     1981 tgcggtgatc ggcaacgggt ttttccccaa tatgccccgg gcttaaccgg ttgcagttac
     2041 aagccaggcg gcgcggcttg cactcggcgc cattactgag ccgtcagtca aagcgccctg
     2101 ccgggatggc cagagctgct agagcatccc aagcacctgc gcccataccg ctacagtggc
     2161 aagaaacagg ctcaacgagg ctagcgcgac cgcttcttcc aagactgacc ggaccatgat
     2221 tgagaccata attgcacccg actgtttaga acataaagag aacatagttc ccttattgtt
     2281 cacggtcaag cgggaagccg ttgccttcac cctcggggca aaagccggtt aatcgaaact
     2341 taacgcccga ggcgcgcccc acatggcgcg ctttggatgc gcgagcggac ggcacgcgct
     2401 tttttcagga gcattttttg gacctatttc cgccatcagc aggaacccgc tggtcgcgcg
     2461 tcatttgaca tagtcagagg ctcgcacatg gccgtcgtgc tgattgtcga ggatgaggat
     2521 caggtccgcg tcctggctga atcttatttg gaagaacaag ggcaccaagt gctctcggcc
     2581 ggaacgccgg ccggcgctct cgcacttctg catcaattgc cggcggtcga tctcttgttc
     2641 acggatctcg atctgaaagg cgacacccac gccggcatcg aattggcaaa ggcggcggtc
     2701 aagctacgcc cgcaactgca tgtgctttat acgac
//
LOCUS       DATLJT010001610         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2917316,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001610 DATLJT010000000
VERSION     DATLJT010001610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2917316"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VM910_36825"
     CDS             complement(<1..286)
                     /locus_tag="VM910_36825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVI68107.1"
                     /translation="MAKNKAPRAVSGGPAKAENGAGPTAGGKAPTFRLPRDGGGTVGL
                     TDFAGRKLVLYFYPRADTSGCTQEAIDFSRLKGAFNRAGTDILGVSADPVA"
     gene            501..>2204
                     /locus_tag="VM910_36830"
     CDS             501..>2204
                     /locus_tag="VM910_36830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68108.1"
                     /translation="MHPRRRASLAAAHRGERWGDSRYLAAYRRLLARHRRLIRRLAIG
                     GGGLVGLLVLGCAGLWWRLSTGPIQVDAFSPWLVAAIEENFGSRERVEVGGTQIERTA
                     NGGAAVRVRDIVVRDPDGTVVASAPKAEIRVSGLSLLSGHMRAESLSLLGAELAVRIE
                     RDGNITVFASGADNHPIASAAPLVAGPLASASLTHSASQQPMPPDKASIGKPAAATLP
                     PGTAVPSRAATPHPAAADSIAAVLAWLDGIGQSGLDGHDLRELGLKDGTLTVDDQRTG
                     KRWSFHNITMSLERPHGGGITVTIGSENSEHPWGLTAAIKPTSDGHRSIALEARQVAA
                     NDLLLASRLDDGTMQVDLPLSASLRGEIGPNGVPQALAGRIVAHAGAISDGDSSDGRI
                     EIDRAEFKINWDAASRLLEVPFQIVSGNNRFTLLGQVEAPAQPQGSWGFKIAGGSVVL
                     TAPAASGEPLVLNRIDVIGRFDPTSKRLTIIHGDAGNADLGVALSGNADYPNGNLRVA
                     TGLAATRMSADTLKRLWPVFVAPKVRDWFNEHLAGGNLERLTIAVNAPFDTLKASGPP
                     LP"
BASE COUNT          388 a          702 c          722 g          392 t
ORIGIN      
        1 ccgcgaccgg atctgccgaa acgccgagaa tgtcggtacc agcgcggttg aatgcgcctt
       61 tgagccgtga aaaatcgatg gcctcctgag tgcagccgct ggtatcggcg cggggataaa
      121 agtacagaac gagcttgcgt ccggcgaagt ccgtaagccc gacggtcccg ccgccatccc
      181 gcggcagccg aaaagtcggc gctttgccgc cggcggtggg tcccgcgcca ttttcagctt
      241 ttgccgggcc gcctgaaacg gcgcgcggag ccttgttttt ggccatgtgc cttcctttcg
      301 acgctttcgg gtcagaattc ttcggctaat tcttggcttc tagaatacgc ccgccgacag
      361 gggtggtcga ttgcggggcg ttaatcctat cggctgcaat atcatcatcg tgacggcgcg
      421 acggacgagg ggacgcccgg gcgggtatgg ccaaccagga taggcagatc ggatcgcctc
      481 cgcgatgggg cgaaagcatc gtgcacccgc ggcgccgggc cagtcttgcg gcggcccatc
      541 gcggcgagcg ctggggcgac agccgttacc ttgccgctta tcgccgcctg ctggcgcggc
      601 atcggcggct tatcaggcgc ctcgcgatcg gcggcggcgg gcttgtcggc ttgctggttc
      661 ttggctgcgc tggactgtgg tggcggctct cgaccggacc tattcaggtc gacgctttca
      721 gcccgtggct ggtcgctgcc atcgaggaaa acttcggcag ccgcgagcgg gtagaagtcg
      781 gcggcacgca gatcgaacgc accgcgaatg gcggcgcggc cgtgcgtgtt cgcgacatcg
      841 tcgtgcgcga tcccgacggc accgttgtcg caagcgcgcc gaaagcggaa atccgcgtct
      901 ccggcttgag cctgctcagc gggcatatgc gcgccgagag tttgagcctg ctcggcgccg
      961 aacttgcggt gcggatcgag cgggacggca atatcaccgt atttgcctcc ggtgccgaca
     1021 accatccgat cgctagcgcc gctcccctgg tcgctggccc gttggccagc gcatcgctca
     1081 cgcactctgc gagccagcaa ccgatgccgc cggacaaagc gtcaatcggc aaaccggccg
     1141 cggcaaccct accgccgggc actgcagtgc cgtcgcgagc cgcgacgccg catccggcag
     1201 cggcggacag tatcgccgcc gtcttggcgt ggctcgacgg catcgggcaa tcggggctcg
     1261 acggccatga cttgcgcgag ctcgggttaa aagacggcac attgacggtg gacgatcagc
     1321 gcaccggcaa gcgatggagt tttcacaata tcacaatgag cctcgaacgg ccgcacggcg
     1381 gcggcatcac cgtgacgata ggttcggaaa attcggaaca tccctggggt ctgaccgctg
     1441 cgatcaagcc gacgagcgac ggccatcgca gcattgcact cgaagcccgc caggtggcgg
     1501 ccaatgatct tctgctcgcc tccaggcttg atgatggcac aatgcaagtc gatctgccgc
     1561 tttcggcgag cctgcgcggc gagatcgggc ccaacggtgt accgcaagcg ctggctggtc
     1621 gcatcgtcgc ccatgcgggc gcaatcagcg acggtgacag cagcgatggt cggatcgaga
     1681 tcgatcgcgc ggagttcaag atcaattggg atgcggcgag ccgtcttctc gaagtcccat
     1741 tccagatcgt ttccggaaac aatcgattca ctttgctcgg gcaagtcgaa gcgcctgcgc
     1801 agccgcaagg atcgtgggga tttaagatcg ccggcggcag cgtcgtgctg actgcgcctg
     1861 ccgcatccgg cgagccgctc gttctcaatc ggatcgacgt gatcggccgc ttcgatccca
     1921 ccagcaagag gctgaccatc attcatggag atgccggcaa cgcggacctc ggcgtggcac
     1981 tgtcaggcaa tgccgactat ccgaacggca atctccgcgt cgctacggga ctagccgcga
     2041 cacggatgag cgccgatacc ctgaaaaggc tgtggccggt cttcgtcgct cccaaggtgc
     2101 gcgactggtt caacgagcat cttgcgggcg gcaatctcga gcgcctgacg attgcggtga
     2161 atgctccatt cgacacgctc aaggccagcg gtccgccgct tccc
//
LOCUS       DATLJT010001611         2789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_846941,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001611 DATLJT010000000
VERSION     DATLJT010001611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846941"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VM910_36835"
     CDS             complement(<1..1114)
                     /locus_tag="VM910_36835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI68109.1"
                     /translation="MRVIFALLLTSLAAGLVNSEAFAADRKVALVISNAKYPDNDFVL
                     NDAINDARDVADELTRDGFEVERGNDLTADATRRALERFYGKIERGSAALIFFDGFGV
                     QSARQTYLLPVDAQIWTEPDVLRDGFALEAILDEMNNRGAAVKVALIDASRRNPFERR
                     FRRYSAGLAPAVTPSNTLVLYSTALGSVVSNSRNDHSLFVTELLRELRVPGINAEQIL
                     RNTQAGVVSASRGEQVPWLSSSLTTEFSFAGPLDPRSDTTIDKGAAKIGTAKLPCEAP
                     QPYPPPNADELAKDAKIDEMSRRIAADRSDKIAFYKRGQLYAIKRAFALAAKDFDETI
                     RLDPRDAEAYNNRCWIRAAVGDLRQALGDCNEALRLK"
     gene            1371..1901
                     /locus_tag="VM910_36840"
     CDS             1371..1901
                     /locus_tag="VM910_36840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HVI68110.1"
                     /translation="MTAYFRRQLADYVEYHRDPRNCAAHVLGIILLFLAAILPLTMWP
                     VPAFGGHITLAVLLALPVLIYWLALDAAVGLAIVVAAVLLFSIASFIVANVSVGAVWG
                     LAAVLAIVGVGSQVVGHKVFERRKPSMADHPTHFLLGPPFVMAKLMIALGFRDDLAAI
                     IGPAAQGGAGASSYPS"
     gene            1929..>2789
                     /locus_tag="VM910_36845"
     CDS             1929..>2789
                     /locus_tag="VM910_36845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVI68111.1"
                     /translation="MTPVLVTGGSGFIGQHLVSELVGRGRQVRVLDVRLPSRMLPAVD
                     YIQGSVLDTDLVQSALAGVGEVYHLAGLPGMWKPDRAEFHAVNYVGTENVLAAAQRCG
                     VARFLHCSTESILFYTPQGDDVAAEEASLSVDHMPGPYTRSKMLADQSALRAAGTGMH
                     VVVGCPTMPIGPHDHNLTPPTAMLQHFLGGGQLQMYLDFVVNLVDVRDAALGLILAME
                     KGRVGHRYIIGGDSLPLSHVLGLMAAVSGRRKYFVPVPGQLAELAASMIEFKANYLTR
                     RTPSATVEGVR"
BASE COUNT          472 a          828 c          879 g          610 t
ORIGIN      
        1 gtttgagccg caaggcttcg ttgcaatcgc cgagcgcctg tcgcaggtcg ccgacggcag
       61 ccctgatcca gcatcgattg ttgtaggctt ccgcgtctcg tggatcgagc cggatggttt
      121 cgtcaaaatc cttcgccgcc agggcaaagg cgcgcttgat cgcgtaaagc tggccgcgct
      181 tgtagaatgc gatcttgtcg ctgcggtctg ccgcgatcct gcggctcatc tcgtcgatct
      241 tggcgtcctt ggcgagttcg tctgcgttcg gcgggggata gggctgcggc gcctcgcaag
      301 gcagcttggc cgtcccgatc ttcgctgcgc ctttatcgat cgtggtatcg gagcgagggt
      361 cgagcgggcc ggcgaaggag aactcggtcg tcagcgagga cgacagccag ggaacctgct
      421 cgccccgact ggcgctgact acaccggcct gggtgttgcg cagtatctgt tcggcgttga
      481 tcccgggcac acggagttcc ctcagcagtt cggtgacgaa caggctgtga tcgttccgac
      541 tgttggagac caccgagccg agcgcagtcg agtacagcac cagcgtattg ctgggcgtca
      601 ccgccggcgc aaggcctgcc gaatagcggc gaaagcggcg ctcgaacgga ttgcgccgcg
      661 acgcatcgat tagcgcaacc ttgaccgccg cgccgcggtt gttcatttca tcgaggatgg
      721 cctcaagtgc aaatccgtcc cttagcacgt ccggctcggt ccagatctgc gcgtcgaccg
      781 gcagcagata ggtttgacgc gcggattgca cgccaaatcc gtcgaagaag atcaaggcgg
      841 cgctgccccg ctcgattttt ccatagaagc gctcgagcgc ccgccgcgtg gcgtcagcag
      901 tcagatcatt gcctctttcg acttcgaagc cgtcgcgcgt gagttcgtcg gccacgtccc
      961 gcgcgtcgtt gattgcgtcg ttcaggacaa agtcgttgtc gggatacttg gcgttgctga
     1021 tgacgagtgc aaccttgcga tcagcggcga aagcctcact attgacaaga cctgcagcaa
     1081 gactcgtgag caacaaagcg aagatgactc gcatgaactg ctcgtcggcg ctgaatccgg
     1141 agttcaggat atctggcagg accaatattt caatcgagaa ccggtcgttt ccgagcctgt
     1201 tcagggttgc atccccgact catcggttgt ggatttttca agacgaatcc ggtcaatttt
     1261 ccttgtctgt tcagcctttt tgacatgggc cggacgggta gcttaataag cagccctgat
     1321 cgcgtctgac cctgtcgtct tggccggacc aaacccggaa agggacccga atgaccgcct
     1381 attttcggcg ccagcttgcg gattacgtgg aatatcaccg cgatccgcga aactgcgccg
     1441 cgcatgtatt gggtatcatc ttgctgttcc tcgctgcgat tcttccgttg accatgtggc
     1501 ccgtcccggc tttcggcggc catatcacgc tggcggtgtt gctggccctg cctgtcctga
     1561 tctattggct tgcactcgac gcagccgtgg ggctcgcgat cgtcgttgcg gctgttctgt
     1621 tgttttcgat tgcgtcattc attgtcgcga atgtgagcgt aggagcggta tggggattgg
     1681 ctgctgtact ggcgatcgtc ggcgtggggt cgcaggtcgt tggacacaag gtattcgagc
     1741 gccgcaaacc gtcgatggcg gatcatccca cgcatttcct gctgggaccg ccattcgtga
     1801 tggcaaagct catgatcgcg ctcggatttc gcgacgatct cgccgcaatt atcgggccgg
     1861 ctgcgcaagg tggcgcgggg gcttcatctt atccgagcta actgtgagat gaaccgaagt
     1921 ctcgattcat gacacctgta ctcgttactg gcggcagcgg tttcatcggg cagcatctgg
     1981 tgtcggagct tgtcggtcgg ggccggcagg tccgcgtcct cgacgtccgc cttccgagcc
     2041 gcatgctgcc cgcagtggat tatatccagg gatcggtgct cgacacggat ctggtgcaga
     2101 gcgcactggc cggtgttggg gaggtttacc atctggccgg cctgcccggc atgtggaagc
     2161 cggaccgggc cgagttccac gccgtcaatt acgtcggcac agagaacgtt ctggcggctg
     2221 cgcagcgatg cggggtcgcg cgatttctgc actgctcgac cgaatccatc ctgttctaca
     2281 cgccgcaagg tgatgacgtc gcggccgaag aggcgtcatt gtcggtcgat cacatgcccg
     2341 gtccttatac gcgctcgaag atgctcgcag accagagcgc gttgcgggct gccggcacgg
     2401 gaatgcatgt cgtcgttggg tgtcccacga tgccgatcgg cccgcatgat cacaacctca
     2461 ctccgccaac cgcgatgctt cagcattttc tgggcggcgg tcagctccag atgtatctcg
     2521 acttcgtcgt gaacttggtc gacgtgcgcg atgccgcgct ggggctcatc cttgcgatgg
     2581 agaaggggcg ggtggggcac cgctacatca taggcggcga cagcctgccg ctcagccatg
     2641 tactggggct gatggcggcc gtcagcggcc gtcgcaagta ttttgttccg gttcccggcc
     2701 aactcgccga actggccgcg tcgatgatag agttcaaggc caactacctg acgcgccgca
     2761 cgccttcggc caccgtcgaa ggcgtccgc
//
LOCUS       DATLJT010001612         1646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_390434,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001612 DATLJT010000000
VERSION     DATLJT010001612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390434"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="VM910_36850"
     CDS             complement(<1..1004)
                     /locus_tag="VM910_36850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI68112.1"
                     /translation="MTIATDTDAGGTKARIDWTQPVLWLFAIVLIALILLPLSWLAVY
                     SLTDKTRHFTLQNFVTLFSDPDFLDPLLTTAIIAVTSSAICCAVAAPIGWLVSRTDMP
                     GRQTIRALVTASFVTPPFLGAVAWELLAAPNSGLLNQFYRTVTGAESDSYLFNIYSLP
                     GLIFVISCYTFPYVFVLVANALDTMPGELEDASAILGAKAWTTARRVTIPLALPALVA
                     GSLIAFLQAMTLFGSPAILALPAGFHTMTTKIWSLFQYPPKLELAAAAAVPLLLLTIL
                     LLQAQKFILGRRGYSVVGGKYGAPRRVEMKGWRWAALAFCLVVLLNPVFLPYFALLNA
                     A"
     gene            complement(1065..>1646)
                     /locus_tag="VM910_36855"
     CDS             complement(1065..>1646)
                     /locus_tag="VM910_36855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI68113.1"
                     /translation="LVKPEDAPKSFADLLDPKWAGKMVKGHPAYSGTIMTATFQLVRE
                     LGWEYFEKLAKQRVMQVQSSTDPPKKLSLGERAVMADGNEYGIVLLKEAGQPVEPVYP
                     TEGTPTISGPTGIFASAPHPNAAKLFQAWLHTRETQQFFVDFTAQYSVHAQVASKAGR
                     RKISDIKLMKEDAAGVEAMSEEIKTRYAKLFRV"
BASE COUNT          320 a          500 c          549 g          277 t
ORIGIN      
        1 gcggcattga gcagcgcgaa atagggcagg aagacggggt tcaggagcac gacgagacag
       61 aaggcaagcg ccgcccagcg ccagcccttc atctcgacgc ggcgcggcgc gccatatttg
      121 ccgccgacca cggaatagcc gcgacggccg aggatgaatt tctgcgcctg cagcagcagg
      181 atggtgagta gcagcagcgg caccgccgcg gccgcggcaa gctcgagctt tggcggatac
      241 tggaacaggc tccagatttt cgtggtcatg gtgtggaagc ctgcgggcag cgccaggatc
      301 gccggcgagc cgaacagcgt catcgcctgc aggaacgcga tcagcgagcc cgcgaccagt
      361 gcaggcagcg cgagcggaat ggtgacgcgc cgcgccgtag tccaggcctt ggcgccaagg
      421 attgcggagg cgtcttccag ttcgcccggc atggtgtcga gcgcattggc gaccagcacg
      481 aatacgtacg ggaaggtgta acaggagatc acgaagatga gccccggcag cgaatagatg
      541 ttgaagagat agctgtcgga ttccgcgccg gtgacggtgc ggtagaactg gttgagcagg
      601 cccgaattgg gcgctgccag aagctcccag gcgaccgcgc cgagaaacgg cggcgtgacg
      661 aacgacgccg tcaccagcgc gcggatggtt tgccgtcccg gcatgtcggt gcgcgagacc
      721 agccagccga tcggcgccgc gaccgcgcag cagatcgccg acgaggtgac ggcgatgatt
      781 gccgtcgtca gcagcggatc gaggaagtcg ggatcggaaa acagcgtgac gaagttttgc
      841 agcgtgaaat gccgcgtctt gtcggtgagg ctgtagaccg caagccacga cagcggcaac
      901 aggatcagcg cgatcagcac gatcgcaaac agccacagca caggctgcgt ccaatcgatg
      961 cgggctttcg tgccgccggc gtcggtgtca gtggcaatcg tcatgctgtc accgcgcggc
     1021 gtgctctggc tcctgaagga gagagcggtt tgcgatcggc atcatcacac ccgaaacagc
     1081 ttcgcatagc gcgtcttgat ctcctcgctc atcgcctcca cgccggccgc atcctccttc
     1141 atcagcttga tgtcggagat cttccgccgc cccgctttcg aggcgacctg cgcatgcacc
     1201 gaatattgcg cggtgaaatc gacgaagaat tgctgggtct cgcgcgtgtg cagccaggcc
     1261 tggaacagtt ttgcggcatt gggatgcggc gcagacgcga aaatgccggt cggccccgaa
     1321 atcgtcggcg tgccttcggt ggggtagacc ggttcgaccg gctgacccgc ttccttcaac
     1381 agcacgatgc catattcatt gccgtcggcc atcacggccc gctcgccgag cgacagcttt
     1441 ttcggcggat cggtggagga ctgcacctgc atcacgcgct gtttggccag tttctcgaaa
     1501 tactcccagc cgagctcgcg cacgagctgg aaggtcgcgg tcatgatggt gccgctatag
     1561 gcgggatggc ccttcaccat cttgccggcc catttgggat cgagcaggtc ggcaaaactt
     1621 ttcggcgcgt cctccggctt caccag
//
LOCUS       DATLJT010001613         4871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4296419,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001613 DATLJT010000000
VERSION     DATLJT010001613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296419"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VM910_36860"
     CDS             <1..999
                     /locus_tag="VM910_36860"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI68114.1"
                     /translation="WVKGVAEALKAGGLDVPALLGEAGFDAAALNDPDARFPTERVSL
                     LWQLAVARSGNPAIGLSTSNVVRPASFDVVAYTMMSSPNLLGILKRLARYVGIVSDAA
                     SVAVTEDHEGCRMTLELFGGGQPVPRQRFEFDLMTILSFCRWVTNHDLRPLALELRFP
                     PPADSQPYRDAFKCPLRFNAAVNGLLFAGADVIAPLPTAHPLLAEVHERLASEHLQRL
                     DHAQTCSRARAAILRRLAGGEPRRTEIARELEMSERTLQRRLQAEGTSFQRLLDDTRR
                     ELAQQYLGQTDLSLADACYLLGFRDLSSFFRTSKRWFGTSPRQYRLRLIRARRVGS"
     gene            1397..1783
                     /locus_tag="VM910_36865"
     CDS             1397..1783
                     /locus_tag="VM910_36865"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HVI68115.1"
                     /translation="MHDAAERLIALDSMASHWIHIVGIGIESFGVLVIVAGIAWSTFR
                     FSQRRMAEQHYDAYKIRIGRSLLLGLEVLVAADIVKTIALELTFTSLGLLAGLVLVRT
                     FLSWTLVLEIESRWPWQREKRPGASS"
     gene            1876..2964
                     /locus_tag="VM910_36870"
     CDS             1876..2964
                     /locus_tag="VM910_36870"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HVI68116.1"
                     /translation="MTDKEDWVDIGSAEDLARTPLKSCKAQAIAIAISFKDGQFGAVS
                     NVCNHVGGPLGEGRLDGDYITCPWHGWKFHRRTGVGEPGYEEDRVPAFPVKVESGRVL
                     VNVSAPSRRTKAPHQPHPLSRKPERAPGPLRLAGISTTVMDMTNPRFSGSDYLLDCAL
                     KSAVDLGAETRLIRLNDLKFRACEGYYSKAAQACTWPCSITQMDARDELDRVYDAFVH
                     WADAVIVASPIRWGTASSLYFKMAERMNCVQNQITIANRVLIRNKVAGFIIVGGQDNI
                     QMVAGQMLGFFAELGFIFPQFPFIAHSRGWSHEDMENNVAMVRNSKELADGACMLAKR
                     LLDLAARLIAQDEAPASIERGGRKAQRV"
     gene            complement(3064..3498)
                     /locus_tag="VM910_36875"
     CDS             complement(3064..3498)
                     /locus_tag="VM910_36875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68117.1"
                     /translation="MDKYLAQRPVFVTSGHAAVSNAIAAGEKLATFDSTSTTPRLKQA
                     GKPIEPVFSRADPTPVFLVGAAIFKHAPHPNAAKLYLTWYLAKEQQSRSGTFSPRSDV
                     APPRGLEPLSSYNIDRGYRALVSDEAGLRELRARFGSYIRRR"
     gene            complement(3499..4101)
                     /locus_tag="VM910_36880"
     CDS             complement(3499..4101)
                     /locus_tag="VM910_36880"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68118.1"
                     /translation="MSSRSTLLRIPALLAASLACCALQARAESMDALYEKAKLEKTVV
                     LYGAGPTGSHDRWIKDFEQRFPGVTVAFTGGLSTSLNQRIERQLASGPMETDLAILQT
                     IQDFGKWKKAGALLLFKPEGFDAIDSAYKDEDGAFTAVSVNTIVYAHNTNLVPSVDVP
                     TSALDFLKSRFQGGLITTDPTEDDAGLAVFTMIVGKYGWD"
     gene            complement(4443..>4871)
                     /locus_tag="VM910_36885"
     CDS             complement(4443..>4871)
                     /locus_tag="VM910_36885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68119.1"
                     /translation="AIGISDTSVSLAFYADILGMTQAYATINRGPAQEALDGTFNAVV
                     RITRLRPIAPDGPGIEFLDYRTPPTGRPAPADTASNDLVHIHLSLRVDALEQQVQSLD
                     GHRARFVSPGVVTLKDGRRAAMVRDPDGHALLLEQGIVDR"
BASE COUNT          865 a         1465 c         1573 g          968 t
ORIGIN      
        1 tgggtcaagg gagttgcgga ggcgttgaag gcaggggggc tcgatgtccc tgcgctcctc
       61 ggcgaggcag ggttcgacgc ggcggcgttg aacgatcccg atgcacgctt tccgaccgaa
      121 agggttagtc ttttgtggca acttgcggta gcgcgttcag gcaacccggc gatcgggctc
      181 tcaacctcga atgtcgtgag accggcgagc tttgatgtgg ttgcatacac tatgatgtcg
      241 tccccgaacc tgctcggcat cctgaagcgt ctggctcgtt acgtcggcat cgtgagcgat
      301 gcggccagcg tcgccgtaac ggaagaccat gaagggtgcc ggatgaccct cgagttgttc
      361 ggcggtggcc aacccgtacc gaggcagcgc ttcgagtttg accttatgac aattctctcc
      421 ttctgccggt gggttaccaa tcacgatcta cgtccgctcg cgctcgaact gaggtttcca
      481 ccgcctgccg attcgcaacc ataccgggat gcgttcaaat gccccctgcg cttcaatgcg
      541 gcggtgaatg gcttgctgtt cgccggcgcc gatgtaatcg cgcccctgcc aacggcgcat
      601 ccgctactcg ccgaggtgca tgagcggctc gccagcgaac atctccagcg gctggaccac
      661 gcccagacct gcagccgagc ccgcgccgcg atccttcgcc gcttggcggg tggtgaaccg
      721 cggcgcacgg aaatcgccag ggaattggag atgagcgaac gcaccttgca aaggcgcttg
      781 caagcggaag gaacctcctt ccagcgtctc ttggatgaca cgcgccgcga gttagcccaa
      841 caataccttg gtcaaaccga cttgtcgctc gcggatgcat gctatctgtt gggtttccgc
      901 gacttgagca gtttcttccg cacgtccaag cgctggtttg ggacttcgcc tcggcaatac
      961 cgtctccgcc tgatacgagc ccgccgcgtt ggttcgtagc tgttcgtggt gcggcgatgg
     1021 gccgtcgccg cgatgctgaa cttggttggg tgctcgttct cggcaacgtc caggtagcgt
     1081 gagagcgcgc cgcggatcgc cggacccgtc tgccgcgtga cgcgcgatca tctcgcggtg
     1141 atgttgggcg gctctgtcat acaatcaaat atggataagc ttttcggcac atacaacaga
     1201 attccgttta atacgaagcg cgcatcaatt cgatgcgaga acggcatttc cgtcgggatt
     1261 gcgcgcagca cacactatcg gtcgcattca ttcggttgtc ggatagggga gagcaccgaa
     1321 ttgccgaact tcaggagcgc ggctcgaccc gcacgaatat tccaggtctt agtcgtttcg
     1381 gatcactgat gccaccatgc atgatgccgc cgaacgtctg atcgccctcg attccatggc
     1441 cagtcattgg atccatatcg tcggtattgg aatcgagtcc ttcggtgttc tagtcatcgt
     1501 cgccggcatt gcctggtcaa cgtttcgctt ttcgcaacgg cggatggccg agcagcatta
     1561 cgatgcgtac aagatccgta ttggtcgatc cctcctgctc ggcctcgagg tcctggtggc
     1621 cgccgacatc gtgaagacga tcgcgttgga gctgacattc acgagtctcg gcctgctcgc
     1681 tggcctcgtg ctggttcgta cctttctcag ttggaccctg gtgcttgaaa tagaaagccg
     1741 gtggccgtgg cagcgggaaa aacgcccggg cgccagctct tgagctaacg gccggcggag
     1801 catcgcagta gactcggtga tggcatctat ccagcaccga gcctgcaaaa gaagcactaa
     1861 cattggggga acgtcatgac cgacaaagag gactgggtcg acatcggcag cgcagaggac
     1921 ctcgcgagaa ccccgctgaa gagttgcaag gcgcaagcca tcgccattgc gatctcgttc
     1981 aaggatggac agttcggtgc ggtatcgaat gtctgcaacc acgtcggtgg gccgctcggc
     2041 gaaggccgtc tcgacggcga ttacatcact tgtccttggc acggttggaa gttccatcgc
     2101 cgcaccggcg tcggcgagcc tggatacgaa gaggatcgcg tgccggcatt tccggtgaag
     2161 gtcgagagcg gtcgcgtgct cgtcaacgtc tcggcgccgt cgcggcgtac caaagcgccg
     2221 caccagccgc atccgctgtc gcgcaagccc gaacgtgcgc cggggccgct gcgtctcgcc
     2281 ggcatctcga cgaccgtaat ggacatgacc aacccgcgct tctcgggttc cgactatctg
     2341 ctcgattgtg cgctcaagtc ggcggtcgat ctcggcgccg agacgcggct cattcggctc
     2401 aacgatctca agttccgcgc ctgcgagggt tactattcga aggcggcgca ggcctgcacc
     2461 tggccttgct cgatcacgca gatggacgcg cgcgacgagc tagaccgcgt ctacgatgcc
     2521 ttcgtgcact gggcggacgc ggtcatcgtt gcctcgccga ttcgctgggg cacggcgtcg
     2581 tcgctctatt tcaaaatggc cgagcggatg aactgcgtcc agaaccagat cacgattgcc
     2641 aaccgtgtgc tcattcgcaa caaggttgcg ggttttatca tcgtcggggg ccaggacaac
     2701 atccagatgg ttgccggcca gatgctcggc ttcttcgccg agctcggctt catctttcct
     2761 cagttcccgt ttatcgctca ttcgcgcggc tggtcgcacg aggacatgga gaacaacgtc
     2821 gcgatggtgc gaaactcgaa ggagctcgcc gacggcgcat gcatgctcgc gaagcgcttg
     2881 ctcgatcttg cggcgcgcct gatcgcgcag gacgaggcgc cggcctcgat cgagcgcggc
     2941 ggccgcaagg cccagagggt gtagatcgga tcacctcacg ttcaaatgca cgggggctat
     3001 tcgtgcttcc gcggcggcgc ctagcggatg tgaacgggag caagatgcgg cagacggctt
     3061 gcctcaccga cgcctaatat aactcccgaa ccgcgcgcgc aattccctta gccctgcctc
     3121 gtccgacacc aaagcccgat agccgcggtc gatgttgtag gaggagagcg gctcgaggcc
     3181 ccgcggcggc gcaacatcgc tgcgcgggga gaaggtgcca ctgcggctct gctgctcctt
     3241 cgccagatac caggtgagat agagcttggc cgcgttgggg tgcggcgcgt gcttgaagat
     3301 cgcggcgccg accaggaaca ccggcgtggg atcggcccgc gagaacactg gctcgatcgg
     3361 cttgcccgcc tgcttgagcc gcggcgtggt cgaggtggaa tcgaaggtcg cgagcttctc
     3421 gcctgcggcg atggcgttcg agacggcggc gtgcccgctg gtgacgaaga cgggcctctg
     3481 cgccaggtat ttgtccattt agtcccagcc gtatttcccc acgatcatgg tgaagacggc
     3541 gaggccggcg tcgtcctcgg tcggatcggt ggtgatgagg ccgccttgaa accggctctt
     3601 gaggaaatcg agcgccgagg tcggcacatc gacggaagga acgagattgg tattgtgggc
     3661 gtagacgatg gtgttgacgc tgaccgccgt gaaggcgccg tcctcgtcct tgtaggcgga
     3721 gtcgatcgca tcgaagccct cgggcttgaa gaggagcagc gcgcctgcct tcttccactt
     3781 gccgaagtcc tggatggtct ggaggatcgc gaggtcggtc tccatcggcc cgctcgcgag
     3841 ctgccgctcg atccgttgat tgaggctggt actcaagccg ccggtgaatg cgaccgtgac
     3901 gccgggaaag cgttgctcga aatctttgat ccagcggtca tgggagccgg tcggtcccgc
     3961 gccgtacagc acgacggttt tttccagctt cgccttctca taaagcgcgt ccatgctctc
     4021 ggcgcgcgcc tgcaacgcgc aacacgcaag cgaggcggcg aggagggcag ggatgcgcag
     4081 gagcgtggag cgtgaactca cattgcttgt cccgatcaga gttgcgtctg cgcgcgctgc
     4141 ggaggccgtt caaaaaccaa acgccggccc agcaatgtcg cggggccggc gcagatttca
     4201 agcacaccat agtcgccatc atcctttggc tggtttggtt ttacggctag gaactgctat
     4261 gcccccaggc tccgaggtgc gaagcgcccc gccgatgtga tcggcgcggc cgtggtggtg
     4321 caggggcgag atcgaagaca cgcgcagcgc cgctgcggta ttctgtgccg tgttgattta
     4381 gcttcggacg gtccgcacga ataccggagc ggctcattgg ggtggctgct gccgtccgac
     4441 ggtcatcgat ccacgattcc ttgctccagc agcagagcgt ggccgtcggg gtcgcgcacc
     4501 atggctgctc gcctgccatc cttcagcgtt acgactccgg gagatacgaa tcgcgcacgg
     4561 tgcccgtcca gagattgcac ctgttgctcc aaggcatcga ctcgcaacga gaggtggatg
     4621 tggacgaggt cattgctggc ggtgtcagcc ggcgcgggcc tcccggtcgg cggcgtgcga
     4681 taatcgagga actcgatgcc gggtccgtcc ggtgcgatcg ggcgcaggcg ggtgatccgc
     4741 acgacggcat tgaaggtacc gtccaaggct tcctgcgcgg gaccgcgatt gatggttgcg
     4801 tatgcctggg tcatcccgag gatatcggca tagaacgcga gactgaccga ggtatcggag
     4861 atcccgatcg c
//
LOCUS       DATLJT010001614         1974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1539745,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001614 DATLJT010000000
VERSION     DATLJT010001614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539745"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VM910_36890"
     CDS             <1..367
                     /locus_tag="VM910_36890"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI68120.1"
                     /translation="GRLHVHRVKNGIPSVHPIRGDEMRALRKLRRDYPTDAHVFVSER
                     GGPISPIGFHRLIQRLGEAAQMPFPIHPHMLRHACGFKLANDGHDTRALQHYLGHKNI
                     QHTVRYTELSPDRFKDFWR"
     gene            complement(393..818)
                     /locus_tag="VM910_36895"
     CDS             complement(393..818)
                     /locus_tag="VM910_36895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68121.1"
                     /translation="MSLPRKRGKGNPHGNRHRTRHLLNQEFMQALLLHFRQHGKKAIE
                     KVARNQPAAYLKILALLVPREMKLEHSGGVKGMTEEQIVEAIEAIEGFLARRAGERAK
                     VIEGEAEVVPSLPAPGTPTTGNERSACSQTAATYRKRQE"
     gene            complement(1089..>1974)
                     /locus_tag="VM910_36900"
     CDS             complement(1089..>1974)
                     /locus_tag="VM910_36900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68122.1"
                     /translation="ARPVHLIVPIAPGGATDIVARLMGQWLSERLGQPFVVENRTGAA
                     TNLGTEAVVRAPADGYTLLLCSTVNATNATLYDKLNFNFIRDIAPVAGVLRGPHVLVV
                     NPSVPAKTVPEFIAYAKANPLKLNMASAGTGNGTHLAGELFKVMADVEMVHVPYRSGG
                     PALTDLLGGQMQVMFPTTVASIEYIRAGRLRALAVTAATRSDALPDTPTVAESVSGYE
                     ASYWFGVCAPKATPAEIVEKLNKETNAGLADRKIKARLADLGGDVLALSPADFEKLIA
                     DDTAKWAKVIRGANIKPE"
BASE COUNT          345 a          637 c          606 g          386 t
ORIGIN      
        1 gggtcgcctg cacgttcacc gggtgaagaa tggaatcccc agcgtccacc caatccgcgg
       61 tgacgagatg cgagcgctgc gcaagctacg ccgcgattac ccgacagacg cccacgtatt
      121 cgtttccgag cgcggcgggc caatcagtcc catcggtttc catcgactca tccagcgcct
      181 cggggaggcc gcccagatgc cgttcccaat ccatcctcac atgcttcgcc atgcctgcgg
      241 gttcaaactg gccaatgatg gccacgacac gcgcgccctg cagcactacc tcgggcacaa
      301 gaacatccag cacacggtca ggtacaccga gctttcgccc gaccggttca aggacttttg
      361 gaggtgacct ggggctcggt cagctcgctt attcactcct gccgcttgcg ataagtcgct
      421 gcggtttggg aacatgccga gcgctcgttt cccgtcgtgg gcgtcccggg cgccggtaag
      481 ctggggacga cttcagcctc gccctcgatc accttggcgc gctcgccggc ccggcgcgcc
      541 agaaaccctt cgatcgcctc gatcgcctca acaatctgct cctcggtcat ccccttgacg
      601 cccccgctgt gctcgagctt catctcgcga ggcaccagaa gcgcaaggat cttgaggtac
      661 gcggccggct ggttacgagc gaccttctcg atcgccttct tgccgtgctg acggaaatgc
      721 agcagcagcg cctgcatgaa ctcctgattg agcaggtgac gcgtgcggtg cctgttgccg
      781 tgagggttgc ctttgccgcg ctttcgggga agggacatga agcaattttt gaatttcacg
      841 cttcccccta ccggggggtt caccacggcg gcgtatctct ctggccctgc tccagccgac
      901 gcaaggtgac tcgacacggg cggcaactag gtggtgcgaa cgcatccgtg gtaggttcta
      961 gtttggccat cgggtttgtc ccgacgccct gactggattt cggcgctaag gggtagcttt
     1021 cagtagccgg gcgcgacagg gataggcaca cgccattcgc ggaacgggat ttatggaatg
     1081 tcgtgtggtc attcaggctt gatgttggcc ccccggatca cctttgccca ctttgccgtg
     1141 tcgtccgcga tgagcttctc gaagtcggct ggcgacagcg cgagcacgtc gccacctagg
     1201 tcggccagcc gcgccttgat cttgcgatcg gcaaggccag cgttggtctc cttattgagc
     1261 ttctcgacga tctcggccgg cgtggccttc ggtgcgcaga cgccgaacca ataactcgcc
     1321 tcgtagcccg acacggactc agccacggtc ggggtgtccg gtagagcatc cgagcgcgtc
     1381 gcggcggtta ctgcgagcgc gcgcagccta ccggccctga tatactcaat cgatgcgacc
     1441 gtggtgggaa acatgacctg catctgcccg ccgagcaaat cggtgagtgc cggtccgcca
     1501 ctacgatagg gcacgtgtac catttcgaca tcggccatca ccttaaacag ctcgccggcc
     1561 agatgtgtcc cgttgccggt tcccgccgac gccatgttga gcttgagggg attggccttg
     1621 gcgtaggcga tgaactcggg aaccgtcttg gccggaaccg atgggttcac caccaggacg
     1681 tggggacctc ggaggacgcc cgcgaccggc gcgatatcgc ggatgaaatt gaaattgagc
     1741 ttgtcgtaca acgtcgcgtt ggtcgcgttg acagtactgc acaggaggag cgtgtatccg
     1801 tcggcaggcg cacgtacaac cgcctcggtg ccgagattgg tggcagcacc cgtccggttt
     1861 tcaacgacga atggctggcc aagccgctcc gacagccact gacccatcaa gcgcgcgacg
     1921 atgtcggtcg cgccgccagg ggcgatcggc acgatcagat gcaccggccg cgcc
//
LOCUS       DATLJT010001615         6837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3609583,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001615 DATLJT010000000
VERSION     DATLJT010001615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609583"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            645..899
                     /locus_tag="VM910_36905"
     CDS             645..899
                     /locus_tag="VM910_36905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HVI68123.1"
                     /translation="MGLNIKNREAHLMAEELAKLTGESMTTAVTIALRERLARVRQQQ
                     GLSLADRLLAIGRDCAARLKEPFRSADHGDLLYDERGLPR"
     gene            896..1327
                     /locus_tag="VM910_36910"
     CDS             896..1327
                     /locus_tag="VM910_36910"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVI68124.1"
                     /translation="MIVHTSALIAILRDEPEALSCAQAIENSSVRRVSAANFVEAAVI
                     IDASRDPVASRRFDDLIKEAQIIIEPVTESQARIAREAYRDFGKGSSHPAKLNFGDCF
                     AYALAKFTGEPLLFKGDDFAHTDIKRQPIKRAVASDLRTKP"
     gene            complement(1440..1688)
                     /locus_tag="VM910_36915"
     CDS             complement(1440..1688)
                     /locus_tag="VM910_36915"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HVI68125.1"
                     /translation="MKKVSARQANHDFSKLLSWVERGEEILITKRSKPVALLSPYRPP
                     RLTPERQAAIEHAIKVMAQGLSWGTDVRAFTRDEMHER"
     gene            complement(1995..2420)
                     /locus_tag="VM910_36920"
     CDS             complement(1995..2420)
                     /locus_tag="VM910_36920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVI68126.1"
                     /translation="MTLYLDTSLFVAALTNETETRRMQAWLGEQEPSDLAISDWVITE
                     FSAAFSMKLRSGQIGAIHRADALAMFARLNVDSFTILLMSGLQFRTAARFADQHALGL
                     RAGDALHLAICADHGATLCTLDRRLSEAGPALGVKTMLL"
     gene            complement(2417..2659)
                     /locus_tag="VM910_36925"
     CDS             complement(2417..2659)
                     /locus_tag="VM910_36925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HVI68127.1"
                     /translation="MPNVNLADAKAHLSKLVDRAAAGDTVRITRRGKPVAQITAVQTP
                     RRRIDLSALRAMTDMMPPQRESARDLLRRLRNEDRY"
     gene            2989..3654
                     /locus_tag="VM910_36930"
     CDS             2989..3654
                     /locus_tag="VM910_36930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68128.1"
                     /translation="MVSEDLPRCGLSKESKKERRLDSALGESRQDSASQPKSRREDWR
                     STYSPSLKLYSKSRTLAGRSPQELELVTVPVSVLARLTTTRGSLPDHPIRPSIKTAEG
                     DEKLLGLLTVGVLVIRASDFEVWYRSERAKGKWPSQRSRLKISGGRPTKQTEPVRNAV
                     IGLVRDGKWSGKISIAKLHRLLIASSDDKVPSPDTLARLVDQLHAETGEAEFFRVKRA
                     QRK"
     gene            4254..5201
                     /locus_tag="VM910_36935"
     CDS             4254..5201
                     /locus_tag="VM910_36935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68129.1"
                     /translation="MYPLVGGPPVWSTENMEAYIELLNEFTQLLEPRDVMELIWTKEA
                     ADASWEAARKAREKNGLPERKYQQRLQVAAEVRRLNGAAETAAAKPASALDHSRGLEA
                     GFKHYQGLDIAQSRAMKRRDNALRLIARWRKGLAPKARALSDKFIAEEALAEHYDAAH
                     LLADAETDNNADEAMEAAPPLAPAGEAAPPLAPKSEAAEAAPPLAFKSEAAEAAPPLA
                     LKSEATEAARPLVPAEDGPINWVGWLTGAEKYEWLALSKGAQKDFKQPVTSKRWLVEK
                     LVVDRKVVRPDQVCPELAQYIPAIAEAAPTVAASAEAPQ"
     gene            complement(5772..6785)
                     /locus_tag="VM910_36940"
     CDS             complement(5772..6785)
                     /locus_tag="VM910_36940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HVI68130.1"
                     /translation="MPQGSPAVTERRVLVTGGAGFIGSHLCERLLERGDEVLCVDNFY
                     TGTRRNVQHLLSNPLFELMRHDVCFPLYVEVDEIFNLACPASPVHYQFDPVQTTKVSV
                     HGSINMLGLAKRVKAKVLQASTSEVYGDPTVHPQKEDYWGNVNPIGVRSCYDEGKRCA
                     ETLFFDYRRQHRLPIKVARIFNTYGPGMHPNDGRVVSNFIIQALRNEDITIYGTGNQT
                     RSFCYVSDLVDGLMRLMQTHDEVTGPVNLGNPVEFTIRQLAETVISLTGSSSKIAYRP
                     LPEDDPRQRCPDIGLAQNLLGWAPRVQLRDGLMKTVEYFERLLRQVPRDERLPARSAN
                     AAG"
BASE COUNT         1456 a         2038 c         2073 g         1270 t
ORIGIN      
        1 ggaccggctc ccccgcacga cgctgccgca acctcggagc catgtccaca gagcgagcac
       61 gtcagcgcca ccgcaaagcg ctcaccaggt cttcccggct aggcgctgga tgctcgagga
      121 ccgtgatgtt gtggatgcct aacgaaccca ccgacgaggt gtctcggatc ccggtctctg
      181 cccgccttgc gaattgccac gctgccagca gtaaggtgca cgagcccacc cgcattccag
      241 gtacgaggag atcgcgcgcc cggtgtgttt ttgcgagggg agccgaaaga gtggcgtagg
      301 cgaggatgtt ggtatcaaag cgaccatcat cgcgcgtgca ttatcctcgc gcgtgaaagc
      361 tcgtacggcc gcgccccagg gtagcccttg tgccatcaca catcgatggc tgcttgcctt
      421 ccgctgtcag ccgggacggc tgataaggcg acaataaaag ccacgtgttt gctccctcgt
      481 ggtcaggatt tcatcgcccc gctccaacgc gaagcaattg gagaacttgt gcttggctcg
      541 acccccagag cgcctgctct tgcgtctcgg ttcggagcct caccttgcgt tttcacgagc
      601 atgatggtag ataggtatat agtggtcggt agaaaggtag aaacatggga ctgaatatca
      661 agaatcgcga ggcccacctg atggcagagg aactggcaaa gttgaccggc gagagcatga
      721 caaccgcggt cacgattgcc cttcgcgagc ggttggctcg cgttcgtcaa cagcagggcc
      781 tcagcctcgc cgatcgactt ctggcgatcg gcagggactg cgcggcgcgc ctcaaggagc
      841 cgttccgttc cgccgatcat ggcgacctcc tttatgacga gcgcggatta cctcgatgat
      901 cgtccatacg tccgcgctaa tcgctatcct gcgcgacgag cccgaagcgt tatcctgcgc
      961 gcaagcgatc gagaacagca gcgtccgccg tgtgtcagcc gctaatttcg tggaagccgc
     1021 ggtcatcata gacgcgagcc gtgaccccgt cgccagccgg cggttcgacg acttgattaa
     1081 agaggcgcaa atcatcattg agcccgtaac cgaatcccag gctcgaattg cacgggaggc
     1141 ctatcgcgat ttcggcaaag gcagcagcca tcctgccaaa ctgaacttcg gcgattgctt
     1201 tgcctatgcg ctcgcaaagt tcacgggtga accgttgctg ttcaaaggcg acgatttcgc
     1261 gcacaccgac atcaaacggc aacccattaa gcgcgctgtt gcgtcagacc tccgcacgaa
     1321 gccgtgaagg tgtcgcggca aagaacgacg acgggagcta cagaggcgcg ccggtctgcg
     1381 gcagcaagat gtgttcatca aagacggcga gtcgaggatg ttggtatcaa agctgaccat
     1441 catcgctcgt gcatttcatc gcgcgtgaag gctcgtacgt ccgttcccca ggatagacct
     1501 tgtgccatca ccttgattgc atgctcgatg gccgcctgcc gttccggtgt cagccgcggc
     1561 gggcgatagg gggacaataa cgccacgggc ttgctccgtt tcgtgatcag gatttcctcc
     1621 ccgcgctcca cccacgacag caatttggag aagtcgtgat tggcttgacg cgcactgacc
     1681 tttttcatgg ccctacctca aaatgtctac ggagttgtac ggcgtcgagg cgagaaactc
     1741 tcactgggcc tcatcatcaa ggatctgcag ctagtttcgc cccagagatc ttggatccgc
     1801 aagaattccc gctgctcctc ggcgataact atctgagcta atgccagtgc atgagctacg
     1861 atgagcaagt cgcttgcacc gagggatcgt cagctgttgg tctcgccaag acggcgtcga
     1921 ttgacaccac tagccgggat cgcgattttt ggaaccctgt ccatgcaggc tctgcaagag
     1981 cggagtacac tcgctcagag cagcatcgtt ttcacgccca gcgccgggcc agcttcactc
     2041 agacgacggt ctagtgtgca cagggttgca ccgtgatcgg cgcagatggc gagatgcaag
     2101 gcgtcgccag cgcgcaagcc caacgcgtgc tggtccgcga accgtgccgc tgtgcggaat
     2161 tgtaagccgg acatcaacag aatggtgaag ctgtcgacgt tcagccgggc gaacatcgct
     2221 agcgcgtcgg cacggtggat ggccccgatc tgcccgctcc ggagtttcat ggaaaaggca
     2281 gcggagaact cggtgatcac ccagtcgctg attgcgaggt cgctaggctc ctgctcgccg
     2341 agccaagcct gcatgcggcg ggtctccgtc tcattagtca gggctgcaac gaacagcgac
     2401 gtatccaaat aaagcgtcaa taacggtcct cgttccgtag tcgacgaagg aggtcgcggg
     2461 cggattcacg ctgcggcggc atcatgtcgg tcatcgcgcg gagcgctgaa agatcgatcc
     2521 gcctgcgagg cgtttggacc gccgtgatct gagccaccgg cttgccgcgc cgcgtaatgc
     2581 gcacggtgtc gcctgcagcg gcccggtcga ccagcttgct cagatgcgct ttcgcatcgg
     2641 caaggttcac gtttggcatt tctagttcct gaccatttag ttggtcgctt atagcacgac
     2701 agggtggcga gccaagcatg gcatggccgc cggacccggc tcgcgcccga ccgagaacga
     2761 gaatggcgac ccgatcaaag ttgcccgcag gcactggaaa gagacgcacg gaataggccg
     2821 gcggcggcct caacgacccg cattacgtcg gccgcatggc gttgccacct cctctgaccc
     2881 aaagaacgac acgcagcgtt cgcgcaagac gacgtaaacc attgagcgtg cccgacggct
     2941 ttctgcgctt ttcggatgcc atcaatcgtt tggatgaggg catgtggggt ggtctccgag
     3001 gacctgcccc ggtgcgggct atcaaaagaa tcgaaaaaag agcgtcgatt ggattcggcc
     3061 cttggcgaga gcaggcagga cagcgcctca cagccgaagt caagaaggga agactggcgg
     3121 tctacgtatt cgccgagcct caagctctat tcaaaaagtc gcactctggc aggacgctcg
     3181 ccgcaggaac tggagctcgt gaccgtgccg gtgagcgttc ttgcccggct tacaaccacg
     3241 cgcgggagcc tccctgatca cccgatccga ccgtcgatca agactgcgga gggcgatgaa
     3301 aagttgctcg ggctactcac ggtcggcgtt ctcgttatcc gtgccagcga ttttgaggtt
     3361 tggtaccggt cggaacgcgc caaaggcaaa tggccctcac aacgctcgag attgaaaatt
     3421 agcggcggac gacccactaa gcagaccgaa ccagtgagga acgcggtgat cgggctggta
     3481 cgcgatggaa agtggagcgg caaaatcagc atcgcaaagc tgcatcggct gcttattgcc
     3541 tctagcgatg acaaggtgcc cagccctgac acgcttgcgc gtctagtcga ccaattgcac
     3601 gccgagacgg gcgaagcaga gttttttcga gtcaagcgcg cgcagcgcaa atagagcgtg
     3661 gacgctttac cgcaaaaccc tgttcctgga tctggcaggg atagctacgt cgggttgatt
     3721 taagcttctg atccccaatg actttctgaa ggtagtgggc cgcagtttgc ccactccggc
     3781 cagggggagg tacgtcacta cacttcgggt aataaataaa aaacggcgaa ggcacgcacg
     3841 cataccgcgg tatcgccgaa agccctacta aaccgaaagg aagtgtcatg attaaagtag
     3901 taaaacaatc cgagccgacg acccgatacg ggcggcagat cgcgtacgac agggagatcg
     3961 tcatctcaat ttgcagaagg ctcctcgagg gagaggatct ccgggatatc tgcgccaagc
     4021 ccccgatgcc catcggaggg gttttcctgt ggtggatcca ggagcacccg gaagcgcgcg
     4081 cgatctaccg ttccgtggag aactttgaat ccgatcgcaa gctggcgaaa gtactgcgtg
     4141 tccctatgga tgctcgtgtc agtgactggg agacccgaat tcgtgccaat tgcgagcgcg
     4201 gctggccagc ggactcagta gatcgacaat acatccgacc cgattggagc aaagtgtacc
     4261 ccctcgtggg cgggccgccg gtgtggagca ccgaaaatat ggaggcctac atcgagctgc
     4321 tcaacgagtt tacgcagctg ctcgagccac gcgacgtgat ggagctcatc tggacgaaag
     4381 aggcggccga cgcgagttgg gaggcggccc gcaaggcccg cgagaaaaac ggtttgcccg
     4441 agcggaagta ccagcagcgg ctgcaagttg cggctgaggt gcggcggcta aacggcgcag
     4501 ccgagaccgc cgcggccaaa ccggcgagcg cgcttgatca cagccgcggc cttgaagccg
     4561 gatttaagca ttaccagggc ctcgacatag cgcagtcgcg cgcgatgaag cgacgcgaca
     4621 acgccctccg cctgatcgcg cgctggcgca agggtctcgc cccaaaagcg cgcgcgctct
     4681 ccgacaagtt cattgccgag gaggcccttg ccgagcacta cgatgccgcg cacctccttg
     4741 ccgacgcaga gaccgacaac aatgcggacg aggcgatgga agcggcgccc ccgctcgctc
     4801 ccgcgggcga ggctgcgccc ccgctcgctc ccaagagcga agctgcggag gctgcgcccc
     4861 cgctcgcttt caagagcgaa gctgcggagg ctgcgccccc gctcgctctc aagagcgaag
     4921 ctacggaggc tgcacgcccg ctcgttcccg cggaagatgg gccgatcaac tgggtcggct
     4981 ggctcacggg tgccgagaaa tacgagtggc tcgcgttgtc gaaaggtgcg cagaaggact
     5041 tcaagcagcc cgtcacgtcg aagaggtggc tggttgagaa gctcgtggtg gatcggaagg
     5101 ttgtccggcc ggatcaggtt tgcccggagc tggcgcagta tattccggcg attgcggaag
     5161 ctgcgcccac agtggccgcc tccgcggagg caccgcaatg agctcagcga agcagatcgc
     5221 cgccaatcgg aaaaacgcgc aaaaaagcaa aggaccgcgc acagctgccg gcaaagcgcg
     5281 cgccagccgc aatagccgca aacacgcatt gtgcactatc agccgcaaca atcctcttta
     5341 tgcgccacgg attgaggcca ctgctcgggc gatctgttct gaggcgacca atgctgagct
     5401 ttgggaacag gcgatgattg tcggcgagtg cacgaccgtt ctggcctgcg cgcaggccga
     5461 acgcatcgcg ctcatcgagc agttgctcgg ggacccctgc gttcacatcg ctgatactca
     5521 gggcgcaggg ggagccgaac tgggggcgtc tcagtgcaag cccgccccac tccgggatga
     5581 gcatgaagcc atgagccttg ccgcacggga ccttaatcgg ctcgaacgct atgaacgccg
     5641 ggctttatca cgccgcaagg gggccatcga gactttcatg gcgatcagcc acataccgta
     5701 caaccaaatt tcaaattttg gcagaacgaa cccaaatcgt gcgggacaag ggccactccg
     5761 atgtttagga cttagcccgc ggcattggcc gagcgagctg gcaggcgctc gtctcttgga
     5821 acctgcctca acagccgttc aaaatactcg acggtcttca tcagtccatc gcgcagctgc
     5881 actcggggcg cccagccgag caggttctgc gctaggccga tgtctgggca gcgctgcctg
     5941 ggatcatcct caggcagcgg ccggtaagca atctttgacg acgacccggt gagggagata
     6001 accgtttctg caagttggcg aatcgtgaac tcgaccgggt ttccgaggtt caccggtccg
     6061 gtcacctcgt catgcgtctg catcaaccgc atgagcccat cgacgaggtc gctcacgtag
     6121 caaaatgagc gggtctggtt gcccgtgcca tagatcgtaa tgtcctcgtt gcgcaaggct
     6181 tggatgatga agtttgatac gacccgcccg tcatttggat gcatgcccgg accataggta
     6241 ttgaaaatcc gcgcaacctt gatcggcaga cgatgctggc gccgataatc aaagaacagc
     6301 gtttccgcgc accgtttgcc ctcatcatag caagaccgca caccgatcgg attgacgttg
     6361 ccccagtagt cctctttctg tgggtgaacc gtcggatcgc cataaacctc cgacgtcgat
     6421 gcctgcagga ccttggcctt cacgcgcttg gcgaggccca gcatgttgat cgaaccatgc
     6481 acgctgacct tcgtggtttg cactgggtcg aactgatagt gcacgggcga tgctggacag
     6541 gcgagattaa aaatttcgtc gacctcgacg tacagcggaa aacagacgtc gtggcgcatc
     6601 agctcgaaca acgggttcga gagcaaatgt tgcacgttgc gtcgggtccc ggtgtagaaa
     6661 ttatcgacac acaagacttc gtcgccgcgc tccagcagtc gttcgcacaa gtgtgaaccg
     6721 atgaaaccgg ctccgccggt tacaagaaca cgacgttccg tgaccgcggg cgagccttga
     6781 ggcattggca gcaatgtttc ggccatttcg atttaacctt ctccgtcgtg ttgagca
//
LOCUS       DATLJT010001616         1717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2229143,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001616 DATLJT010000000
VERSION     DATLJT010001616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229143"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..>1717
                     /locus_tag="VM910_36945"
     CDS             522..>1717
                     /locus_tag="VM910_36945"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HVI68131.1"
                     /translation="MQTGREFAQGSGGVVVASLLGAIICFTTIGALAEEPLKFPRSHL
                     EPIKWSELAGWTADDHLAAFAAYQTSCQALLKIRRTDERGELSAALSNVCRKAANLQP
                     QDTETARAFFEQNFQPLRIGRLGEVKGLLTGYFEPIVAGSRFPTAEFHVPLYRRPRDL
                     VAAGYKPGSVAFPNKGVRIGRRNENNELVPYHDRSAIEAGALDGQRLEICWLRDPFDL
                     LAIQLEGSGRVILEDGTPLRVSFDSHNGYPYSSIERVLIDRNLIPRNAISPQGIRDWM
                     AAHPDVAAKVRAANRSYVFFRVTGLTNEGEPVGAQGVPLTPGRSIAVDRVHEYGTPFF
                     IEADLPIEGRKLASPFHRLMIAQDTGSAIVGPARADLYWGAGDEAGRIAGRIRHPARF
                     VMLLPR"
BASE COUNT          308 a          542 c          528 g          339 t
ORIGIN      
        1 cggcggacgt tttagcgggg tatgtgtcca aaatgtggtc acaagcacta aattctttcg
       61 ctgatggatt cagggatgca aatcctaaag ttccaggttc cgtcgagtcg cttcgactga
      121 cgaagttcga ctgacgaaga ataatcgggt ccgatcacgc ctccccttta aaaaatcggg
      181 cgtgaatttt ttccggcctg gctcggagcg gctcgccgca ctggccgtcc gtcacgcggt
      241 gccgacggtc ttcgaaaacg cgagttcatc gcggccgggg gattgatggg ttatggtggt
      301 agcttcgctg acacttaccg tctggcaggc agccgatatc gggatggtcc atggcggaaa
      361 atagttaacg cgcgtcttgc tgaaaagttg cactcgaacg atttctgttt tttgccagca
      421 tcgcgatacc gggcgccagc gcggcaaagc cattcaaccg ctgccaaaga gatcgtcgcg
      481 cgcgtagtgg aaccattcct gtctgcggtg catttgttgg catgcagact ggtcgggagt
      541 ttgcgcaagg gtctgggggc gtcgtcgtcg caagcctcct tggcgcgatt atctgtttca
      601 cgaccatcgg cgcattggcc gaagaaccgt tgaaattccc gcgcagccat ctcgagccga
      661 tcaaatggag cgagctcgcg ggctggacgg ccgacgatca tctcgccgct ttcgcagcct
      721 atcagacaag ctgccaagcc ctgctcaaga tacgacgcac ggacgagcgc ggagagctct
      781 ccgccgcgct atcgaacgtc tgccgcaagg ccgcgaacct tcagccgcag gatacggaaa
      841 ctgctcgcgc cttcttcgag cagaactttc aacccctgcg catcgggcgt ctgggcgagg
      901 tcaagggact tctgaccggc tatttcgagc cgattgttgc gggctcgcgc tttccgactg
      961 ccgaatttca tgtcccgctc taccgccggc cgcgtgatct ggtcgcggcc gggtacaagc
     1021 ccggctccgt ggcatttccg aacaaaggtg tgcgcatcgg tcgccgcaac gagaacaacg
     1081 agctcgtgcc gtaccacgac cgcagcgcca tcgaggccgg cgcgctcgac gggcagaggc
     1141 ttgagatctg ctggctcagg gatccgttcg acttgctcgc gatccagctc gaaggctcgg
     1201 ggcgcgtgat cctcgaggac ggcacgccgc tgcgcgtcag ctttgattcg cacaacggct
     1261 atccctatag ctcgatcgag cgcgtgctca tcgaccgcaa cctcatcccg cgcaacgcaa
     1321 tctcgccgca gggtatccgt gactggatgg ccgcgcatcc ggacgtagcc gcaaaggtgc
     1381 gcgccgccaa ccgctcctac gtattcttcc gcgtcaccgg gctcacgaac gagggcgagc
     1441 cggttggcgc gcagggggtg ccgttgacgc cgggccgctc cattgccgtc gaccgggtgc
     1501 atgaatacgg cacgccgttc ttcatcgaag ctgatctgcc gatcgagggc cgcaagctcg
     1561 cctcgccctt ccaccgcctg atgatcgcgc aggatacggg atcggcgatc gtcggaccgg
     1621 cgcgcgccga tctctactgg ggcgccggcg acgaggccgg tcgcatcgcc ggccgcattc
     1681 gccatcccgc ccgcttcgtg atgctgcttc cgcgcga
//
LOCUS       DATLJT010001617         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_160635,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001617 DATLJT010000000
VERSION     DATLJT010001617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160635"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VM910_36950"
     CDS             complement(<1..1589)
                     /locus_tag="VM910_36950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68132.1"
                     /translation="MSQLVQYSEWLIPVPVLIAIAWVRFNSPPTNRSGTTFVMFSVGL
                     MLYCLLIGVLWLLVIIGLTQGSLGFNKLHGEFAQHAPLVAALVMVAATHVSWIRRMDD
                     AARAFCITFAAIPREADRLALELAQTAAFQPKTERLRAKVGKVITECVGPHALNFELD
                     GSLAARFTRAVGLYWLFIGPKSGGTPIEFANGNGKSAYTRIMQISEAKAKSAAARYHE
                     LMQAGCAYFATQNPAKELEEALNHSISEVAQLTCNLIARYVLYCDATKSKRLRRLSTM
                     GFDASGAIWSFGPDQWVTTIFAVIGLSAGMMAFTPGMLRLTGGQILTISIPFGLSIGV
                     AVIAAVWVAQRFLEHHDGEISTYPPTAELTAAALIVAGFSAGLRIATPLVPAIIEGSD
                     SALWDALAQFAERWPGVIIPFACTISLGLLCIYLGARPWSPLRVAVLGALANGLACMA
                     AAVVVAWLLQDAVLAKFYKDLEYARVLILLNSGLIGVAIGSMVLWQFKRSERARHDDA
                     EHAAERAREGIPRLAAVEPPEG"
     gene            complement(1902..2273)
                     /locus_tag="VM910_36955"
     CDS             complement(1902..2273)
                     /locus_tag="VM910_36955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68133.1"
                     /translation="MRARTGRTRARLIAMAMVAIFAGIAQAQEDRVEAECRNLLHRSV
                     FNCGCTAEFLEDHLGGEQADILVRLWTYGENGGNQHDEMLDLYRQYGSEKISEAVMNF
                     HRHRDDLRRYCAQGDGPTVSD"
     gene            complement(2480..>2969)
                     /locus_tag="VM910_36960"
     CDS             complement(2480..>2969)
                     /locus_tag="VM910_36960"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI68134.1"
                     /translation="NLQLMIDVLSLGRSWPSPRFHATTTDCYTGERLVLSQSSNIPIS
                     HAVCASASLPGVFGPTWIGDRLCMDGAMCSTSTHVDLIAGAKRALVVALTDKGPRFSN
                     IPNDIEQELRYVEAAGTKTLLIAADPGKVDLLSPAEIEPALTAGRDRAVREADRVKAF
                     WA"
BASE COUNT          565 a          926 c          951 g          527 t
ORIGIN      
        1 ccttctggcg gctcgacagc ggcgagccga gggataccct cccgcgcgcg ttcggccgcg
       61 tgctccgcgt catcatggcg ggcacgctcc gatcgcttga actgccacag caccatggag
      121 ccgatcgcga cgccgatcag accgctattg agcaatatta ggacgcgtgc gtactccagg
      181 tccttgtaaa acttagccag aaccgcgtct tgtagcagcc atgcgacaac cacagccgca
      241 gccatgcacg cgaggccgtt cgcgagcgct cccaacaccg cgacgcggag gggactccag
      301 ggccgtgcac ccagataaat gcagaggagc ccgagcgaga tcgtgcaggc gaatggaatg
      361 atcacgcccg gccagcgttc tgcgaactgc gcgagcgcat cccatagcgc ggaatcgctg
      421 ccctcgatga tggcaggcac gagcggcgtg gcaattcgca accctgcgga gaagccggcg
      481 acaatcagcg cggcggccgt cagctccgca gtcggcggat aggtcgaaat ctctccgtcg
      541 tggtgctcca agaagcgctg tgccacccag acggcggcga tcactgcgac gccgatcgaa
      601 aggccgaacg ggatcgagat cgtcaggatt tgaccgccgg taagccgcag catccccggc
      661 gtgaacgcca tcattcccgc gctgagcccg atgacggcaa aaatcgtcgt cacccattgg
      721 tccggtccaa agctccagat cgcgccgctc gcatcgaacc ccattgtgga cagccgccgg
      781 agccgcttgc tcttggtcgc gtcgcaatag aggacgtagc gcgcgatcag attgcaggtg
      841 agctgcgcaa cttcgctgat gctgtggttc agcgcctcct ccagctcttt ggcaggattc
      901 tgcgtcgcga aataggcgca gccggcttgc atgagctcgt ggtagcgcgc ggcggcgctc
      961 ttcgccttgg cttcgctgat ctgcatgatc cgcgtatagg cggattttcc gttgccgttc
     1021 gcaaactcga ttggcgtgcc gccgctcttg ggtccaataa acagccagta aagcccaacc
     1081 gcccgcgtga accgtgcggc gagcgagccg tccagctcga agttcagcgc atgcggcccg
     1141 acgcactcgg tgatgacttt cccgaccttg gcgcgaagcc gctcagtttt cggttggaag
     1201 gcggcggttt gcgcgagttc gagggccaac ctgtcagcct cgcgcgggat cgccgcgaac
     1261 gtaatgcaga acgcgcgtgc cgcgtcgtcc atccgtcgga tccaggagac gtgcgtcgcc
     1321 gctaccatga caagggccgc caccagcgga gcgtgttggg cgaattctcc gtggagcttg
     1381 ttgaaaccga ggctgccctg ggtgagcccg attatgacca gcagccacaa caccccgatc
     1441 aagaggcaat agagcatcaa cccgacggag aacatcacga aggtcgtgcc cgagcggttg
     1501 gtcggcggtg agttgaagcg tacccaggcg atcgcgatga gcaccggcac tgggatgagc
     1561 cattcgctgt actgcaccag ctgtgacaca agaacctccc gttcgagccg gaggtgttat
     1621 tgataattgt tatgcctccg gcgaggccgc gaaatgggaa ggaggcgggc gaaatcgaaa
     1681 tggctgtgcg ttcgcgcact gcccgcgact tccgacggca aaatatgccg aattgccgga
     1741 gcgctatgtg agccggttca cactcggtcg cgtgaaaaat tctgcgcatc ggcggcgacg
     1801 ccgccgatgc ggtcaagatt tacgcgtgca tgatgcggaa ttgtctcgcg tagctctcgt
     1861 atgccgcgcc ggacacagcg gacgaacacc gggttccgcg cttaatccga gaccgtgggt
     1921 ccgtcaccct gcgcgcagta gcggcgcaga tcatcgcgat ggcggtggaa gttcatcacc
     1981 gcctcggaga tcttctccga gccgtactga cggtagagat cgagcatctc gtcgtgctgg
     2041 ttgccgccgt tctcgccata agtccagagc ctgaccagga tatcggcctg ctcgccgccc
     2101 agatgatctt ccaggaattc ggccgtgcat ccgcaattga agacggagcg gtgcaagagg
     2161 ttgcggcatt cggcttcgac gcggtcctct tgcgcctgcg cgatgccggc gaaaattgcg
     2221 accatcgcca tggcaatcag ccgcgcgcgg gtgcgccccg tgcgggcacg caatcgcgcg
     2281 ccagcggggc ggtgtccgct tgtttgagtt tgcgccgatc gcgatgaacg cgcccaggcc
     2341 cgcagcatgc gggcggtcta ccagcgagcg gcgcgacaag cttgcaggcg gccccgagct
     2401 tccttggaga ttttctggag tttgtatcca acgcggcaat gaaataccgc tccgcgcgcg
     2461 gtcacggtgc tggcgaatat caagcccaaa acgctttgac ccgatcggcc tcgcgtacgg
     2521 cgcgatcgcg gcctgctgtc agcgccggtt cgatctcggc aggagacagc agatctacct
     2581 tgccggggtc ggcggcgatc aggagggtct tggtccccgc ggcttcgacg tatctcagtt
     2641 cctgctcaat atcgttcgga atgttggaaa accgcggacc tttgtcggtg agcgcgacca
     2701 ccaaggcacg cttggcgccg gcgatgagat cgacgtgggt gctggtggaa cacatggcgc
     2761 cgtccatgca cagccgatcg ccgatccagg tcggcccgaa gacgcctggc aacgacgcgc
     2821 tcgcgcacac cgcatgcgag atcggaatgt tgctcgattg cgagagaacg aggcgctcgc
     2881 cggtgtagca atccgtcgtc gtcgcgtgga accgcggact cggccaggag cgtccgagac
     2941 tgaggacatc gatcataagc tggaggttg
//
LOCUS       DATLJT010001618         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4525988,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001618 DATLJT010000000
VERSION     DATLJT010001618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4525988"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VM910_36965"
     CDS             <1..228
                     /locus_tag="VM910_36965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68135.1"
                     /translation="KRGTPIQSQNSHDGSSLIVVLKKSSAGQQHADTAHPLALLRPRR
                     KRPGSRAAEQRDEAAYFVVLPIRSRDPAGVA"
     gene            complement(235..>3042)
                     /locus_tag="VM910_36970"
     CDS             complement(235..>3042)
                     /locus_tag="VM910_36970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI68136.1"
                     /translation="LAVRIGIATGVVVIGDLSREGALREHAVVGETPHLAARLQALAE
                     PGTIVVAASTRRLLGDLFRLRDLGRHEVKGIAEPVAAWAVEGVSASESRFEAVRAAGL
                     TDLIGREHEINFLLERQRLAWKGEGQIVLISGEPGIGKSRLAAALAERITGEPHTRLR
                     YQCSPYHINSALRPFIAQLERAAGFKADDTPEQRLDKLKAVLAMDRSRIEAVVPLFAA
                     LLYPALALSPAQQRRRTLAALLDQFEGLARRQPILLSFEDAHWADATSLELLDLTVER
                     VRQLPVLALFTFRPEFEPPWVGLPNVGTLTLGRLDRDDVENMVAQVTRGRVLPGEVMK
                     QIVVKTDGNPLFVEELTKAVLETGLLVEDAEGYRLDGPLPPLSIPDTLQDSLMARLDR
                     LAPVREIAQIGAAIGREFSYPLVRALVGRDETALKDGLAQLEQAELVFRRGEPPDAVY
                     SFKHVLVRDAAYESLLRSRRHQLHGQIARALEQSFADIAASQPEIVAHHFTAAGLVEP
                     AIDYWLKAVQQAARRSANAEALNHLARGLELLPNIDDPMLRNKSELLLQTSLGNSLRA
                     TKGWSIDSVKHAYTRALQLCKESGFDEHTLPAVFGLWTWNFVRAALGEAQSLAERLVN
                     IAENVDDSVYKVLAHEALGFTLFARGKFAYAHAALERSISMCEDSKAAAYLDLSAQDP
                     RVHVRLYDGMALWFLGYPDQALRICDEARRYAETSQHPFSEAMARTISLRVHQFRGEG
                     AVVARQANAAIALCEEHGFVHYLAMALILRGWASAQQGEFEKGIAEAQEGLERERAID
                     ALLLESYTLGLLTDACIKNERYRQALDFLNQAQLRLDDENSERFYAAEIYRLLGETYL
                     RSRQDLDQAERCLCKGLEIAREQKAKSLELRLCVSIYDLYELRQNADKYRPQLGEIYG
                     SFIEGFDTTDLVRAKARLQNA"
BASE COUNT          605 a          948 c          889 g          600 t
ORIGIN      
        1 aagcgcggca caccgattca atctcaaaac tctcatgatg ggtcctccct tatcgtggtc
       61 ttgaagaagt catcagcggg gcagcagcat gccgatacgg cgcatccgct cgccttgctg
      121 cgcccacgcc gcaagcggcc aggcagtcgc gccgccgagc agcgtgatga agccgcgtat
      181 ttcgttgtcc tgccaatacg cagccgcgat ccggcagggg ttgcttaatc ctaatcaagc
      241 attttgcaac cttgctttgg ctctgactag atccgtggtg tcgaaaccct cgatgaaaga
      301 tccgtagatc tcgccgagtt gcgggcgata tttgtcggcg ttttgtctca gctcgtatag
      361 gtcataaatg ctcacgcaaa gtctaagttc gagggatttg gctttctgtt cgcgcgcgat
      421 ctcaaggccc ttacacaaac aacgctctgc ctgatccagg tcttgacgcg atcgcaggta
      481 tgtttctcca agcaagcgat atatttcggc cgcgtaaaag cgttcggagt tctcgtcatc
      541 gagcctcaat tgcgcctggt tcaaaaagtc caacgcttgc ctgtaacgtt cattctttat
      601 gcatgcatcc gtcagcaatc ccagagtata cgactcaagc aataaggcat cgatggcccg
      661 ttctctttcc aaaccttcct gagcctcggc aatacctttc tcaaactcgc cttgctgggc
      721 acttgcccat ccgcgcaaga tcaaagccat ggccagatag tgcacaaacc catgctcttc
      781 gcagagtgcg attgccgcgt tagcctggcg tgcgacaacg gcaccctcac cgcgaaactg
      841 atgtactcgg aggcttatcg ttcgtgccat tgcctcgctg aatggatgtt gagatgtctc
      901 tgcataacgg cgggcctcat cgcaaatccg cagcgcctga tcaggatagc cgagaaacca
      961 gagtgccatg ccgtcgtata accttacatg aactcgcggg tcttgcgctg acaggtcgag
     1021 atacgccgcg gccttgctgt cctcacacat gctaatgctg cgttctagcg cggcgtgggc
     1081 ataggcaaat ttgccgcgtg caaataaggt aaaccctaaa gcttcgtggg caagaacctt
     1141 gtagaccgag tcatctacat tctcggctat attcaccaaa cgttcggcaa gggattgagc
     1201 ttcacccagc gctgcgcgaa cgaaattcca ggtccataaa ccaaacaccg caggcaaggt
     1261 atgttcgtca aacccactct ctttgcacag ctggagtgct cgcgtatacg catgcttcac
     1321 actgtcaatg ctccagccct tagtcgcccg gagggaattg ccgagcgatg tttgaagcag
     1381 caactccgac ttgtttcgta acatcgggtc gtcaatattg ggaaggagct ccaaaccgcg
     1441 cgcaagatgg tttagtgcct cggcattggc cgagcgtcgg gctgcctgct gtacagcctt
     1501 gagccagtaa tcgatggcgg gttcaacaag gccggctgcc gtgaagtggt gcgccacgat
     1561 ttccggctgg ctcgccgcga tgtcggcaaa gctctgctcg agtgcgcgcg caatctggcc
     1621 gtgcaattga tgccggcggc tccggagcag gctctcataa gccgcatccc gcaccagcac
     1681 gtgcttgaag ctgtagacgg cgtccggcgg ctcgccccgg cggaacacga gttccgcctg
     1741 ctcgagttgg gcgagcccat ccttcaatgc ggtctcgtcg cgtccgacca acgcgcgcac
     1801 caatggatag gagaactcgc gcccgatggc ggcaccgatt tgggcgatct ccctcaccgg
     1861 tgccaggcgg tcgagccgcg ccatcagaga gtcttgaagc gtgtcaggga tggatagcgg
     1921 cggcagcggc ccgtcgagcc gataaccttc ggcgtcttcg accaggaggc ccgtttctag
     1981 caccgccttt gtaagttctt cgacgaacag cgggttcccg tcggtcttga ccacgatttg
     2041 tttcatcact tcgccgggca gcacgcgccc cctggtcacc tgcgcgacca tgttttcaac
     2101 gtcgtcgcgg tcgagtcgac cgagcgtcaa ggtgccgacg ttgggcagac caacccaggg
     2161 cggctcgaac tccggtcgga aggtgaacag tgcaaggacc ggtagctggc gcacccgctc
     2221 gacggtcaga tcgagcagct cgagggaggt ggcatccgcc cagtgcgcat cctcgaacga
     2281 gagcaggatc ggttgccggc gggcgagacc ctcgaattga tcgagcagtg ctgccagcgt
     2341 ccggcggcgc tgctgcgcgg ggcttagcgc cagcgccgga tacaggagcg cagcaaaaag
     2401 aggtactacg gcttcaatcc gcgagcggtc catagccagc accgctttca acttgtcgag
     2461 gcgctgctcg ggcgtatcgt ctgccttgaa cccggcggct cgctccaact gggcgatgaa
     2521 cggacgaagc gcactattga tgtgataggg cgagcattgg taccgcaggc gcgtgtgcgg
     2581 ctcgccggtg atgcgctcgg cgagagccgc cgcaaggcgg gacttgccga tccccggctc
     2641 gcccgagatc agcacaatct gcccttcgcc cttccaggcg aggcgttggc gttccagcaa
     2701 gaaattgatc tcgtgctcgc ggccaatgag atcggtcaga cccgctgcgc ggaccgcctc
     2761 gaagcggctc tcggaagccg acacgccctc gaccgcccac gccgcgaccg gctcggcaat
     2821 acccttgacc tcgtgcctgc cgaggtcgcg cagacggaac agatcgccga gcagccgacg
     2881 cgtggaggca gcgacgacga tcgtcccggg ctcggccaag gcctgaaggc gggctgcaag
     2941 gtgcggcgtc tccccgacca cagcgtgctc ccgcaacgca ccctcgcggc tgaggtcgcc
     3001 gatcaccacc acgccggtcg cgatgccgat gcgaaccgcg ag
//
LOCUS       DATLJT010001619         4438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3149789,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001619 DATLJT010000000
VERSION     DATLJT010001619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149789"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VM910_36975"
     CDS             complement(<1..864)
                     /locus_tag="VM910_36975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI68137.1"
                     /translation="MTERTPRPAPAADAVPVQAPKAEITALAANANLVPPLPWPPRRL
                     SLALQGGGSFGAFAWGVLERLLEEPGCEIGAISGASVGAVNAALVACGLVKGGREGAR
                     RKLTQFWNRITNEVSFRSLMLIGGFSPAGSSVAFGPTLRSGKFDPLDLDPLREQLKRH
                     IDFSAFREATCPRLLIAATRVRDGRLKIFGNRDITADVLLASSCPPLVHCAVEIEGES
                     YWDGGYAANPPVLGLVQAPESADVLVVQVTPSRDSYIPVTMAAIDRRLDQIAANAALN
                     AEIAGFELALGG"
     gene            complement(1320..1913)
                     /gene="plsY"
                     /locus_tag="VM910_36980"
     CDS             complement(1320..1913)
                     /gene="plsY"
                     /locus_tag="VM910_36980"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HVI68138.1"
                     /translation="MGPEAFLPVAFIIGYFFGSIPFGLILTRLAGTADLRSIGSGNIG
                     ATNVLRTGRKGLAAATLLLDMLKGTAAVVIAGYFGGPNSAMLAGLGAFLGHLFPVWLN
                     FRGGKGVAVYIGVLLGLFWPAAVVFCLIWLAVALSFRYSSLSALVASFATPLFLWWFG
                     HLALASLFAVLTVLLFYMHRENIKRLQAGTEWRIGQK"
     gene            complement(1946..3247)
                     /locus_tag="VM910_36985"
     CDS             complement(1946..3247)
                     /locus_tag="VM910_36985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HVI68139.1"
                     /translation="MLTDRRPILLANARVIDPSRDFDGPGDVLIADGVIRDLKRGIGA
                     AGVPEGTDVINCAGKIVAPGLIDMRAFVGEPGFGHRETFASASQAAATGGITTIICQP
                     GTQPVIDNSATVDFVLRRARDTAIVNIHPMAALTKGLAGEVMTEIGLLKAAGAVAFSD
                     GDKSVTNAQVMRRALTYARDFDALIVHHTEDPDLVGEGVMNEGEFAARLGLMGIPDAA
                     EAVMLERDIRLVALTGGRYHAASLSCIASLEILKRARDAGLAVSASVSINHVTLNEND
                     IGPYRTFLKLSPPLRSENDRKALVEALASGLIDVIMSDHNPQDVEVKRLPFAEAASGA
                     VGLQTMLPAALRLIHNGEMDWKTLIRAMSTRPAQLLGLQGGSLRPGAPADIIVIDADT
                     PWVLDPADLKSLCKNTPFDEARFSGRVVRTIVGGRTVYEHV"
     gene            complement(3412..4365)
                     /locus_tag="VM910_36990"
     CDS             complement(3412..4365)
                     /locus_tag="VM910_36990"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVI68140.1"
                     /translation="MTSTSKSTFVLGHRHLLGIEGLSAADITGLLDLSEEYVELNRQI
                     DKKRTSLRGRTQVNLFFEASTRTQSSFEIAGKRLGADVMNMSVSSSSMRKGETLIDTA
                     VTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
                     RIEGLIIAICGDVLHSRVARSNILLLNIMGARVRVVAPSTLLPPGIERMGVEVARDMR
                     EGLEGADIVMMLRLQRERMNGSFVPSSSEYFHYFGLDQKKLAYAKPDALVMHPGPMNR
                     GVEIDSLVADGAQSLIREQVEMGVAVRMAVLEALARNLPNA"
BASE COUNT          837 a         1415 c         1429 g          757 t
ORIGIN      
        1 ccccccaagc gccagctcaa agccggcgat ttcggcgttc agtgccgcat tggccgcgat
       61 ctgatcgaga cggcggtcaa ttgcggccat ggtgacggga atgtaactgt cgcggctcgg
      121 cgtgacctga acgacgagca catcggcgct ctcgggcgcc tgcaccaggc cgagcaccgg
      181 cggattggcg gcatatccgc cgtcccaata ggattctccc tcgatctcga ccgcacaatg
      241 caccagcggg ggacagctcg atgcgagcag cacatcagcg gtgatgtcgc gattgccgaa
      301 gatcttgagc cgcccgtcgc ggacccgcgt cgcagcgatc agaagccttg ggcaggtcgc
      361 ttcgcgaaac gcggaaaaat cgatgtgtct cttgagctgc tcgcgcagcg gatcgagatc
      421 caacgggtcg aacttgccgc ttcgcaaggt cgggccgaac gcgaccgagc ttcccgccgg
      481 cgagaagccg ccgatcagca tcagcgaacg gaacgaaact tcgttcgtga tccggttcca
      541 gaattgcgta agctttcgcc gcgctccctc gcggccgccc ttgaccagcc cgcaagcaac
      601 aagtgcggca ttgaccgcgc cgacgctcgc accgctgata gcgccgattt cgcaacccgg
      661 ttcttccaga aggcgttcca gcacgcccca ggcgaatgca ccgaaggagc cgccgccttg
      721 caaggcgagc gaaagtcttc gcggaggcca ggggagaggg gggacaagat ttgcgtttgc
      781 ggcgagcgcg gtaatttccg ctttgggcgc ctgcacaggg acagcatcgg ctgcgggcgc
      841 aggccggggc gtgcgctccg tcacgggctg cgattcccgg actggttcct ggattcgcct
      901 gccgctcgaa ctgaagaacc gtgcctccgc caaaagctct gacggtcgcc gcttgggtgc
      961 ggttttctgc gggctcgcgg ttttctcgga aatttccgcg ggagccttga cagctacgga
     1021 cgcttcggcc ggcgcagggg tctcctcggg cgccgaccag atttcggcgg ctgagagcag
     1081 caagctcgcc gcacttcgca gcggtgtagc cgcaggctct gccgcgcccg cccggtccaa
     1141 tccgtcattc gcgcctgatg aatcgctgtc gctcatccgc ggcaacccag acccacgcga
     1201 actaccccgc tcgcgaccat ggcaggcgcg tcaccgcttc gccagagtct ccgctgagtt
     1261 gcgaacagga tttgtgttgt gttggtgcag tcttggctcg agtgctgctc tgttgcgtct
     1321 tacttctgcc cgatccgcca ttcggtgccc gcctgcagcc gtttgatgtt ctcgcgatgc
     1381 atatagaaca ggagcacggt caacaccgcg aacaacgagg ccagtgcgag gtgaccgaac
     1441 caccacagaa acagcggcgt ggcaaagctt gcgaccagcg ccgacagcga ggagtagcgg
     1501 aaggagaggg cgaccgcgag ccagatcagg caaaacacca ctgcagccgg ccagaacagc
     1561 ccgagcagca cgccgatata gacggcgacg cctttgccgc cgcgaaagtt tagccagacc
     1621 ggaaagagat ggcccaggaa cgcgccgagg cctgccagca tcgccgagtt aggcccgccg
     1681 aagtaacccg cgatcacgac cgcggccgta cccttgagca tatcgagcaa gagcgtcgcc
     1741 gcggcgaggc ccttgcgacc ggtgcgcagc acattggtgg cgccgatatt gccggagccg
     1801 atcgaacgca gatcggccgt gccggcgagc ctggtcagga ttagaccgaa cggaatcgag
     1861 ccaaagaaat agccgatgat gaaggcgacc ggcaaaaagg cttcaggtcc catgggaggc
     1921 gcaccttccg catattttcc gcgcatcaga catgctcgta gacggtccgt ccgccgacga
     1981 tggtgcgcac cacgcgtccg gaaaatcgcg cttcatcgaa cggcgtattc ttgcagagcg
     2041 atttgaggtc ggcggggtcg agcacccagg gcgtatcggc gtcgatcacg atgatatcgg
     2101 cgggcgcgcc ggggcgtagc gagccgccct gcaagcccag cagttgcgcc ggccgggtgg
     2161 acatggcacg gatcagggtc ttccagtcca tctcgccatt atggatcaag cgcagggcgg
     2221 ccggtagcat ggtctgcagg ccgaccgcgc cggaagcggc ctccgcaaat ggcagccgct
     2281 tgacctcgac gtcctgcgga ttgtggtcgg acatgatgac atcgatgagg ccggaggcga
     2341 gcgcctccac caacgccttg cggtcgttct cgctgcgcaa cggcggcgac agcttcagga
     2401 aggttcggta ggggccgatg tcgttctcgt tcagcgtcac gtgattgatc gagaccgagg
     2461 ccgagaccgc aagccccgca tcgcgcgcgc gcttcaaaat ctccagagag gcgatacagc
     2521 tcagcgatgc cgcgtggtag cgcccgccgg tcagcgccac gagccgaatg tcgcgctcga
     2581 gcatcaccgc ctctgcggca tcggggatcc ccatcagccc aaggcgcgcg gcaaactcgc
     2641 cctcgttcat gacgccttcg ccgacaagat cggggtcttc cgtgtgatgc acgatcagcg
     2701 cgtcgaaatc gcgggcgtag gtcagggcgc gccgcatcac ctgcgcgttg gtgacgctct
     2761 tgtcgccatc ggagaaggcg accgcaccgg ccgctttcaa caacccgatt tcggtcatca
     2821 cctcgccggc gaggcccttg gtaagtgccg ccatcggatg aatgttgacg atcgcagtgt
     2881 cgcgggcgcg ccgcagcacg aaatccaccg tcgccgaatt gtcgatcacc ggctgcgttc
     2941 ccggctggca gatgatggtc gtgatgccgc cggtcgccgc ggcctggctt gcggaagcaa
     3001 aagtttcgcg gtgaccaaag ccgggctcgc cgacaaaggc gcgcatgtcg atcaggcctg
     3061 gcgcgacgat cttgccggcg caattgatga cgtcggtgcc ttcgggcacg ccggccgcgc
     3121 cgataccgcg cttcaggtcg cgaatgacgc catccgcgat cagcacgtcg cccgggccgt
     3181 cgaaatcgcg cgacggatcg atcacgcgcg cgttggcgag caggatcggg cggcggtcgg
     3241 tcaacatggg catctagtgt cccggctcta acgttcgcat cccctgtgcg gcgaacgttc
     3301 ttgcgaacgt cggaaccgaa gggacgccag cgaatcttat ccgacctggt gcacttgcga
     3361 agctccctgg agaactcgcg gagccactgt aggaacttcg cattccgcgc actaggcgtt
     3421 aggcaggttg cgggcgagcg cttcgagcac tgccatccgc accgccactc ccatctcgac
     3481 ctgttcccgg atcagcgact gcgcgccgtc ggcaacaagc gagtcgattt cgacaccgcg
     3541 gttcatcgga cccggatgca tcaccagcgc atcgggcttc gcgtaggcga gcttcttctg
     3601 gtcgagcccg aaataatgga aatactcgct cgatgacggc acaaaggaac cattcatccg
     3661 ttcgcgctgc agtcgcagca tcatgacgat gtctgccccc tcgagtccct cgcgcatgtc
     3721 gcgcgccacc tcgaccccca tccgctcgat gcccggcggc agcagggtgg agggcgcgac
     3781 cacccgcacc cgtgcgccca tgatgttgag cagcaggata ttggagcgcg cgacgcgcga
     3841 atgcaggaca tcgccgcaga ttgcgataat aagcccttcg atccggccct tgttgcggcg
     3901 gatggtcaag gcatcgagaa gcgcctgggt cggatgttcg tgagcaccgt caccggcatt
     3961 gatcacggaa ccgtcgacct ttcgtgccaa aagttccacc gcgcctgagg cgtggtggcg
     4021 caccaccagg atgtccgggt gcatggcgtt caacgttacg gcggtgtcga tcagggtctc
     4081 gcccttgcgc atcgaggacg aggagaccga catgttcatg acgtcggcgc cgagccgctt
     4141 tcccgcgatc tcgaaggagg attgggtacg cgtcgaggcc tcgaagaaca ggttgacctg
     4201 ggtgcggccg cgcagcgacg tgcgcttttt gtcgatctgg cgattgagct cgacatattc
     4261 ctcggagagg tcgagcaggc cggtaatgtc ggcagccgaa agcccctcga ttcccagcag
     4321 gtgccggtga ccgaggacga aggtcgattt cgatgtggat gtcattaaag ccagagctat
     4381 aggcggggat ttctggcagg gcaagggcga aagccggtcg aaaaagttat ccccgggt
//
LOCUS       DATLJT010001620         1575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4067530,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001620 DATLJT010000000
VERSION     DATLJT010001620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4067530"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..751)
                     /locus_tag="VM910_36995"
     CDS             complement(245..751)
                     /locus_tag="VM910_36995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HVI68141.1"
                     /translation="MSFEIERKFLMRGNDWEKLAVRRTSLRQAYLASNGKVSIRIRIK
                     DDRAATLTVKSRPADLRRLELEYDIPVLEAEALIKLRQGSIIEKVRHVIPCGDVVWEV
                     DVFSGDNFGLVIAEVELQHEHQHVELPPWVGAEITGQPQYYNSSLVQRPFCSWSSQEA
                     PMPIERRA"
     gene            complement(963..>1575)
                     /locus_tag="VM910_37000"
     CDS             complement(963..>1575)
                     /locus_tag="VM910_37000"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HVI68142.1"
                     /translation="LINGRIHLGAARLIYAGLVIVAISTGLLLGLALLGVSLPVDQAG
                     RAVPLWQDVIAAGVAIAAYSVFFSTPPNMLLWPVTVGMLAHALRWVAITVIGFGAAIG
                     TLLACLVVGLILTPVARRRHMPFAAIGFASVVSMLPGVLLFRMASGLVQIAGGAQTSL
                     ELISATIADGMSALISILAMSLGLITPKLVIDDLSERAKQIKS"
BASE COUNT          337 a          484 c          501 g          253 t
ORIGIN      
        1 cggcggctgg ggctttcagt tctgggaggg cggagatccc aagaagccgc aggtgaccga
       61 cgccgccaaa cagtgcttcg cgtgtcacca gccgaaaaaa gaccaggact acgtctattc
      121 cacctacatt ccgtgaagcg gagataagga ccgcggcgaa gtcgcgatac cgctcagcgc
      181 caacccgacg acgttctccc cacgccgcgc caaacggcgc gagcccacaa gcctactcac
      241 gcgttcacgc ccgcctttca atcggcatcg gcgcttcctg actcgaccac gagcaaaatg
      301 gacgctgcac caaggagctg ttgtaatact gcggctgtcc ggtgatctcc gcgccgaccc
      361 agggcggcaa ttcgacgtgc tgatgctcgt gctgcaactc gacttcggcg atgaccaaac
      421 cgaaattgtc gccggagaaa acatcgactt cccagacgac atcgccgcac gggatgacat
      481 ggcgcacctt ctcgatgatg gagccctggc ggagctttat cagcgcctcg gcttccaaca
      541 ccggaatatc atactccagc tccagccggc gcaggtccgc ggggcgagat ttgacggtga
      601 gcgttgcggc gcgatcgtcc ttgatgcgaa tgcggatcga tactttcccg ttgctcgcca
      661 aataggcttg tcgaagggag gtgcggcgga cggcgagctt ttcccaatcg ttgccgcgca
      721 tgaggaattt tcgctcgatc tcgaaactca ttgcctcccc tcgactgggc tatgattgca
      781 acaggcgggc tctggatgac gaccggcaga aacggattat gacggtcacg tgacactcta
      841 agacgctcga atatcaaaag aaaaagataa aagcaagcgg aggttgcgag gcgcggatgc
      901 gatcggcaag gccggagatc gatggcgtcg gatgcgtgag ggtcggcgta gtccttcgcc
      961 tgctatgact ttatctgctt cgctcgctcg ctcaggtcat cgatcacgag cttcggagtg
     1021 atgaggccca agctcatcgc caagatgctg atcagagcgc tcatgccgtc ggcgatggtc
     1081 gcgctgatca gctccagcga ggtctgcgcg ccgcccgcga tctgcacaag accgctggcc
     1141 atccggaaca ggaggacgcc cggaagcatc gacaccacgg aggcgaagcc gatggcggca
     1201 aacggcatgt gccgacggcg tgcgaccggc gtgaggatca gcccgacgac gagacacgcg
     1261 agcagggtgc cgatcgcggc gccgaagccg atcacggtga tcgcgaccca gcgcagcgcg
     1321 tgcgccagca tgccgaccgt aaccggccaa agcagcatgt tcggcggggt ggagaagaag
     1381 acgctgtaag cggcgatggc gacgccggcg gcaatcacgt cctgccacaa cgggacggcc
     1441 ctgccggctt gatcgaccgg cagggacacg ccgagcagcg cgaggccgag cagcaatccc
     1501 gtcgagatcg cgacgatgac gagccccgcg tagatcagcc gggcggcgcc gagatggatg
     1561 cggccgttga tgagg
//
LOCUS       DATLJT010001621         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2001652,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001621 DATLJT010000000
VERSION     DATLJT010001621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001652"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VM910_37005"
     CDS             complement(<1..1034)
                     /locus_tag="VM910_37005"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI68143.1"
                     /translation="MIIRNIRTHVLAAPLSQPFAYARAWYDTRTAMLVEIETDSGLVG
                     WGECYGPARVNSAVVKEIAPWLIGEDPLRTDHLWQSVYARLRDHGQKGVVIQGLSGID
                     IALWDIKGKHFGVPAHRLLGGPLRSEVRAYATGLYRRKSGDPGKYLAEEAASYVAEGF
                     GAVKLKVGFGIEEDAAMACAVRAAIGRDVALMVDANHAYDAVAAIRLGRMIEELDIGW
                     FEEPVLPEDIAGYRAVKAAITIPVAGGECEFTRFGFRDLLASRAIDIVQPDTCAAGGL
                     SECKKIADMAEAFSVRYNPHVWGHRDCDCRIFAASRRAAIAHADLVGAGRADARIRSD
                     RAPDPPVDPG"
     gene            complement(1080..2741)
                     /locus_tag="VM910_37010"
     CDS             complement(1080..2741)
                     /locus_tag="VM910_37010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVI68144.1"
                     /translation="MKNRITGRSAFLALLKSEGITHLFGNPGTTELPIMHALKDHPDL
                     TYVMAMQESLVVAIADGFSRASGKLVACNVHVAPGLGNALGSLFNAKFTGTPMILTAG
                     QQEQGHGLMEPVLYGPLVQMAEPLVKWAVEVTRLEDLPRIVHRAAKIATTPPTGPVFI
                     SLPGDILNTEAGIDLGRSTRVDTRVKPSDEARQALVQRILKAQRPVIVTGDEIVKSDA
                     LNEAAALAEALGAPAYQCSTPYGAHFLSESPCFMGALSRLQKQVREVLSPYDLMIVLG
                     ADPLRMSVYSEVDPMPDGLTMIQIGLVDWDLAKNYGAEIALKADVKETLRALVPALKA
                     AGGAALETRAKRGVAELASKNWTAKRKALIEQIAKAKDKSPIDPDYLTLGLVEAMPEN
                     AILVDEGLTSGRQILALRPHRDRYGYHALASGGIGWGVPASVGVSLANPARPVVCFSG
                     DGSAMYSIQALWTAAHHKLPLNVVIANNGGYRIIKQRLLAFHGDDHYVGMDFVDPPVD
                     FSGLARSLGLEAIRIGDPKELKPKLSDAFKRPGAKLIEVMVDGSV"
BASE COUNT          507 a          942 c          886 g          512 t
ORIGIN      
        1 tcaccaggat cgactggcgg atcgggtgct cggtccgatc gaattcgagc atcggctcga
       61 ccggcgccaa cgaggtcggc gtgtgcgatg gcagcacggc gagaagctgc aaagatgcgg
      121 caatcgcaat cccggtgccc ccaaacatgg ggattgtagc gaacactgaa agcctcggcc
      181 atgtcggcga tcttcttgca ttcgctgagc ccgcccgcag cgcaggtgtc cggctggacg
      241 atgtcgatgg cgcgcgatgc caagaggtcg cggaagccga agcgcgtaaa ttcgcactcc
      301 ccgccggcga cagggatcgt aatggctgcc ttcaccgccc gataaccggc gatgtcctcc
      361 ggcagcaccg gctcttcgaa ccagccgata tcaagctcct cgatcattcg gccgaggcgg
      421 atcgcggcga ccgcatcata ggcgtggttg gcatcgacca tgagcgcaac gtcgcgcccg
      481 atcgccgcgc gcaccgcgca cgccatcgcc gcatcttcct cgatgccaaa accgactttc
      541 aacttcacgg ccccaaagcc ttccgccacg tagctcgccg cttcctctgc gagatatttt
      601 ccggggtcgc ccgatttgcg gcggtaaaga cctgtggcgt aagcccgaac ctcgctgcgg
      661 agcggcccac ccagcagccg atgcgcggga acgccaaagt gctttccctt gatgtcccat
      721 agcgcgatgt cgatgccgct cagtccctgg atcaccacgc ccttctgacc gtgatcgcgg
      781 agtcgcgcgt aaacggattg ccagaggtga tcggtacgca gcggatcctc gccgatcagc
      841 cacggcgcaa tctccttgac cacggcggaa ttgacgcggg cgggaccgta gcactcaccc
      901 cagccaacca ggccgctgtc ggtctcgatc tcgaccagca tggcggtgcg cgtgtcgtac
      961 caggctcgcg cgtaagcgaa gggctgggac aacggcgctg cgaggacgtg ggtgcggatg
     1021 ttcctgatga tcatgcgtca gatccatttt ccgggtcaaa ccgacacatg gctagcttct
     1081 cacaccgacc cgtcgaccat cacctcgatc agttttgcac cgggacgctt gaatgcgtcc
     1141 gacagtttcg gcttcagttc ttttggatcg ccgatccgga tcgcctccag cccaagcgat
     1201 ctggcgaggc ccgagaaatc aaccggcgga tcgacgaaat ccatgccgac gtaatgatcg
     1261 tcgccgtgga aggcgagcag ccgctgcttg atgatgcgat agccgccgtt gtttgcgatc
     1321 accacattga gcggcagctt gtgatgcgcc gccgtccaca gcgcctggat cgagtacatc
     1381 gcgctgccgt cgccggagaa acacacgacc ggccgcgcgg ggttggcgag actgacgccg
     1441 accgaggccg gcacgcccca gccgatgccg ccggaggcaa gcgcatggta gccatagcgg
     1501 tcgcgatgcg gccgcagcgc caaaatctgc cgccccgagg tgaggccttc gtcgaccagg
     1561 atcgcgttct cgggcatcgc ctcgacaagc ccaagcgtca gataatccgg atcgatcggc
     1621 gatttgtcct tggccttcgc gatctgctcg atcagcgctt tacgtttcgc cgtccagttt
     1681 ttcgaagcga gttcggcaac gccccgcttt gcccgtgtct ccagcgcagc cccgccggcc
     1741 gccttgagcg ccggcaccag cgcgcgcaag gtctccttca cgtcggcctt cagcgcgatc
     1801 tcggcgccgt agtttttcgc aagatcccag tcgaccagac cgatctggat catcgtcagg
     1861 ccgtccggca tcggatcgac ctcgctgtag acggacatgc gcaggggatc ggcgccgagc
     1921 acgatcatga ggtcgtaggg cgagagcact tcgcgtacct gcttttgcag ccgcgacagc
     1981 gcgcccatga agcacggact ttccgaaagg aaatgcgcgc cataaggcgt cgagcattga
     2041 taggccggcg cgcccagcgc ctcggccagc gcggcggctt cgttcagggc gtcgctcttc
     2101 acgatctcgt cgccggtgac gatcaccggc cgctgcgcct tcagaatgcg ctgcacgagc
     2161 gcctgccgcg cttcgtccga cggtttgacg cgggtgtcga cgcgcgtcga gcgtccgagg
     2221 tcgatgccgg cttcggtgtt gaggatgtcg ccgggcagcg agatgaagac cgggccggtc
     2281 ggcggcgtgg tcgcgatctt tgccgcgcgg tgcacgatgc gcggcaaatc ttccagccgc
     2341 gtcacctcga cagcccattt caccaatggc tccgccattt gcaccagcgg gccatagagc
     2401 accggctcca tcaacccgtg gccctgctcc tgctggcccg cggtgaggat catcggcgtg
     2461 cccgtgaatt ttgcgttgaa gagcgagccc agcgcattgc cgaggcccgg cgcgacatgg
     2521 acgttgcagg caacgagctt gcccgaagcg cggctgaacc cgtcggcgat tgcgacgaca
     2581 aggctttcct gcatcgccat cacataggtg aggtcgggat ggtccttcag cgcatgcatg
     2641 atcggcaatt cggtcgtgcc gggattgccg aacaaatggg tgatgccctc gctcttcaac
     2701 agcgcgagaa acgccgagcg accggtgata cggttcttca tgtctcctcc ctccccgccg
     2761 ccggtggcgc gggctttacg gcatcatgct gatcccgatg ccgagatcaa tggaggagga
     2821 tcatggctgc cttgcgcggc gggccac
//
LOCUS       DATLJT010001622         9484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_391864,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001622 DATLJT010000000
VERSION     DATLJT010001622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391864"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VM910_37015"
     CDS             complement(<1..158)
                     /locus_tag="VM910_37015"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HVI68145.1"
                     /translation="MPQVKLSDLNACSKADFVAALANIFEYSPWIAEQAADARPLAGV
                     KALFEAMKA"
     gene            complement(165..1100)
                     /gene="puuE"
                     /locus_tag="VM910_37020"
     CDS             complement(165..1100)
                     /gene="puuE"
                     /locus_tag="VM910_37020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HVI68146.1"
                     /translation="MAEGRYPRDLRGYGRKPPDPRWPGDARVAVQFVVNFEEGGENNI
                     LHGDRASEAFLSDVLGAQPWPGKRHPNIESMFEYGSRAGFWRLWRIFTERRLPTTVFG
                     VATALQRNPEVVAAMKEAGWDIASHSLKWIEHKDMSEAEERREIAEAIRVHTEATGAR
                     PLGWYTGRTSINTIRLLMEAGGFLYLCDSYADDLPYWIKGPTEPQLIIPYTLDANDMR
                     FVNAQGFGGGDQFFTYLKDSFDVLYAEGATSPKMMSVGLHCRLVGRPGRAASLMRFLD
                     YVLKHERVWVPTRLEIAQHWHANLRHLAADAFDIG"
     gene            complement(1245..1658)
                     /locus_tag="VM910_37025"
     CDS             complement(1245..1658)
                     /locus_tag="VM910_37025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HVI68147.1"
                     /translation="MSQLIVRAGDFTFEARFEEQLAPKTVAAFRKAMPFESQAIHVRW
                     SGEGVWMPLGDLDFGVSYENHTSYPAPGQIILYPGGISETEILLAYGGVHFASKVGQL
                     AGNHFITLTSGLENLTAFGKTVLWKGAQKIRFEEV"
     gene            complement(1774..2064)
                     /locus_tag="VM910_37030"
     CDS             complement(1774..2064)
                     /locus_tag="VM910_37030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68148.1"
                     /translation="MKKQVVAILLMSSCIAATPSLAQTKQTPKASSRVDNCAPIGRTA
                     NGELVYSMKCETLPAPSPPPPQAEVKETPPPEPEVRRSGLFGWSYDRRQPDQ"
     gene            2299..3915
                     /locus_tag="VM910_37035"
     CDS             2299..3915
                     /locus_tag="VM910_37035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68149.1"
                     /translation="MDFTRLEINRRMALLTSAAIAANVIDPMRAFAQETPRKGGVFNV
                     HYGAEQRQLNPSLQASTGVYIIGGKIQENLVDLDSEAKPVGVLAESWEASPDGMTVTF
                     KLRKGITWHDGKPFTSADVAFTAMNMWKKILNYGSTLQLFLTSVDTPDPQTAIFRYER
                     PMPLNLLLRALPDLGYVSAKHLYESGDIRQNPHNLAPVGTGPFKFVQYERGQYVIAER
                     NPDYWRPNSPYLDRIVWRVITDRAAAAAQMEAGELHYSPFSGLTISDMARLAKDKRFV
                     VSTKGNEGNARTNTIEFNFRRKELSDIRVRRAIAHAINVPFFIDNFLGDFAKLGTGPI
                     PSTSTDFYPGPDTPQYPYDKAKAIALLDEAGYKPGAGGQRFSLRLLPAPWGEDISLWS
                     TFIQQSLSEVGIPIDIVRNDGGGFLKQVYDEHAFDLATGWHQYRNDPAVSTTVWYRSG
                     QPKGAPWTNQWGWEDATVDRTIDDAATEIDPIKRKALYARFVKEVNTELPVWMPIEQI
                     FVTVITAKARSHSNTPRWGSTSWHDLWLSA"
     gene            4016..4993
                     /locus_tag="VM910_37040"
     CDS             4016..4993
                     /locus_tag="VM910_37040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68150.1"
                     /translation="MRILSLAGRRLAASIPTLILILIGVFLLLQFAPGDTVDAMMAQM
                     GGGDAATARELRHFYGLDLSIPVQLGNYLWRLVRLDLGFSAIYGKPVATVILERLPPT
                     ILLMTAALSFAFFFGLLFGVAAARGVNKWPDTLISTLGLIFYATPSFWFGLMAIVVFS
                     VYLQWLPPGGFEDIGNVQTGIWRVIDIARHLILPALTLGLIFLAIYLRIMRASMLEVL
                     NLDYVRTARAKGLDETAVITRHVLRNALLPMVTLIGLQAGTMLGGSVVVESVFSLPGL
                     GRLAYESVVQRDLNTLLGIVFVSALLVIAVNFIVDLLYARLDPRITVDG"
     gene            4993..5823
                     /locus_tag="VM910_37045"
     CDS             4993..5823
                     /locus_tag="VM910_37045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68151.1"
                     /translation="MDAARRYFRSPAAVAGLILLLVVIAMAISATWLYPRDPLSLAGR
                     PLLWPFSNPRFPLGTDNSGRDIAAQIFYGARISLLIGGVATAIAIAIGILVGAFAGYY
                     GGRVDNVLMRITEAFQTLPNFILLLVLVAVFGSTLTTVTIAVGVVAWPAPARLTRAEF
                     LSLRNREFVQAGRTLGMSDIQLILGEILPNALPPVVVYASVVMAVAILLESALAFLHL
                     SDPNVASWGNLIGLGRDVLRVQWYVSAIPGVAILVTVLAVSLVGQGLNDALNPRLKGR
                     "
     gene            5820..7451
                     /locus_tag="VM910_37050"
     CDS             5820..7451
                     /locus_tag="VM910_37050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68152.1"
                     /translation="MSEDAPILSLEGLSVRLPAGADRSHALSNVSLSVAANEILCVVG
                     ESGSGKSVMANAIMRLLPPEVRISGGRMVFEGRDLAQATAAAMRAVRGEHISMIFQEP
                     MTALNPLRTIGDQIAEMFSVHTDLSKADIRAKVLSLLADVRIAEPAAAAKAYPHELSG
                     GQRQRAMIAMALALHPKLLIADEPTTALDVTTQAQILKLIRDLQQRRNTAVLFITHDF
                     GVVAEIADRVVVMQRGMIVEQGSASSVLNNPQHAYTRQLIAAVPPLKAPPPRKLAAED
                     ILTMSGLSKTYRTGGFLGRGARVTAAVKDVSLNLPRGATLGIVGESGSGKSTLARCIV
                     RLIDPDNGAIMLDGKDWAALSRKDVRRETRHIQMVFQDPFASLNPRRRAAELVAQGPI
                     VHGTPRTEAIAEARNLFTLVGLDPSAGDRFPHEFSGGQRQRIGLARALALKPDVLVAD
                     EPVSALDVSVQAQVLRLLSELRERFGLSIIFITHDLRVAAQICDLVAVMRDGAIVEHG
                     RTSDVFGNPQHPYTQALLASIPGGDFTRRRDAANA"
     gene            7638..7883
                     /locus_tag="VM910_37055"
     CDS             7638..7883
                     /locus_tag="VM910_37055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68153.1"
                     /translation="MRKTILTIFASALLAGTMIEAATAAEHHNVRRATRTAPVVVGEP
                     FRDSYAYDRNAYGSSYPDYSYWTSHLEGGAISAPAGH"
     gene            complement(7897..9201)
                     /locus_tag="VM910_37060"
     CDS             complement(7897..9201)
                     /locus_tag="VM910_37060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI68154.1"
                     /translation="MNSTNVHWPESLWAAVTPAGPELPELAGAQEADVVVIGGGFTGL
                     STALHLRDAGLDVAVVEAMEPGWGASGRNNGQVIPTLSRPDPEDIIAKHGAAGERFVA
                     LLRDSASILFDLTRRYNIPAEQEQTGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLS
                     RDQVRDMLGSDAWFGGFWNRSGGHINPLALSRGLAGVVVEKGARIFARSPATSIARRD
                     GKWLVKTALGQISGKALVVATNAYSDEFSKSLIPDLAREVVPVLSWQMATQPLSDNVR
                     KTIIPGRQAMSDTHGELYFARYDARNRLVTGGAVIGPGNKAERIKARVTERLQRLWPQ
                     IGDVSFDYVWNGYVGMTTDFLPRMHRLGPDAYGWTGCNGRAVALSIALGGELAKAVHG
                     AEERDLALPFTEPMPIPARSLLRRLAPLMLLVYRRRDAREMA"
     gene            9296..>9484
                     /locus_tag="VM910_37065"
     CDS             9296..>9484
                     /locus_tag="VM910_37065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68155.1"
                     /translation="MALKNVIGIDHAVVMVRDLDKAAENWKRLGFTVSPRGTHSAHMG
                     SGNYTIMLDPDYIELLGVL"
BASE COUNT         1750 a         3049 c         2822 g         1863 t
ORIGIN      
        1 gccttcatcg cctcgaataa tgccttgacg ccggcaagcg gacgcgcgtc ggccgcctgc
       61 tcggcaatcc atggcgaata ctcaaagata ttggcgagcg ccgcgacgaa atcggctttg
      121 ctgcatgcgt tgagatcgga aagcttcact tgcggcatgt caactcatcc gatgtcgaac
      181 gcgtcagcgg cgagatgcct gaggttggca tgccaatgct gcgcgatctc gagccgcgtc
      241 ggcacccaga ctcgttcatg cttcaggaca taatcgagaa accgcatcaa tgatgccgcg
      301 cggccaggcc ggccgaccag ccggcagtgc aggccgaccg acatcatctt tggcgaggtc
      361 gcgccttcgg catagagcac gtcgaaacta tccttgagat aagtgaagaa ctgatcgccg
      421 ccgccaaagc cctgcgcgtt gacgaagcgc atgtcgttgg catcgagcgt ataggggatg
      481 atgagttgcg gctcggtcgg acccttgatc cagtacggca gatcgtcggc ataggagtcg
      541 cacagataaa ggaagccacc tgcttccatc agaagccgga tcgtattgat agaggtgcgc
      601 ccggtgtacc agccgagcgg tcgcgctccc gtcgcctccg tatgcacgcg gatcgcttca
      661 gcgatctcgc gccgctcctc ggcctccgac atgtccttgt gttcgatcca tttcaggctg
      721 tggcttgcga tgtcccagcc cgcctccttc attgcagcaa ccacttcggg attgcgttgc
      781 aatgcggtcg caacaccgaa cacggtggtc ggcagcctgc gctcggtgaa gatgcgccac
      841 aagcgccaga agccggcgcg cgagccatat tcgaacatcg attcgatgtt cggatgacgt
      901 tttcccggcc aaggctgtgc gcccagcacg tccgacaaaa acgcctccga ggcgcggtcg
      961 ccgtgcagga tgttgttctc gccgccttct tcgaaattca caacgaactg cacggcaacc
     1021 cgtgcgtcgc ctggccagcg tggatcgggc ggcttgcggc cgtagccacg gaggtccctc
     1081 ggatatcgcc cctcagccat ccagtgtccc gatccgaagt tcgcttgatg ctgcggcatc
     1141 ctctcgagcg aacttcggac tcgaaaggac accggaaaac ttgagattcc agcggagctt
     1201 tgcatttgat gttcgcataa aggacgaacg tcaaatccgc tccgctagac ttcctcgaag
     1261 cggattttct gcgcgccctt ccacaacacg gttttgccga aggcagtcag gttctccagt
     1321 cctgaggtaa gcgtgatgaa gtgattgccc gcgagctggc cgaccttgct ggcgaaatgc
     1381 acgccgccat aggcgagaag aatttcggtt tcactgatgc cgccgggata aaggatgatc
     1441 tggccgggcg cgggatagct tgtgtgattc tcgtaggaga cgccgaaatc gaggtcgccc
     1501 agcggcatcc agaccccctc gccgctccag cgcacatgga tcgcctggct ctcgaacggc
     1561 atcgccttgc gaaatgcggc cacggtcttg ggcgccaact gttcttcgaa gcgggcctcg
     1621 aaggtaaagt caccggcgcg aacgatcagt tggctcatgc atctctctca tggtcgaagc
     1681 ggcttgtcgc tatccattgc agccaaacgc aatggaggca aggaccattc caacccttgc
     1741 cggggcaccc tcgccctccg acacaatctg ggcctattgg tcgggctgcc gtctgtcgta
     1801 ggaccagccg aacaggccgc tgcgacgcac ttcaggctcc ggcggcggcg tctccttgac
     1861 ctccgcctgc ggcgggggcg gcgacggcgc cggcagggtt tcgcacttca tcgagtagac
     1921 cagctcgcca ttggccgtcc ggccgatcgg tgcgcaattg tcgacgcgcg aactcgcctt
     1981 cggcgtctgc ttggtctgcg ccagcgacgg cgtggccgcg atacaggacg acatcaggag
     2041 gattgcgacg acctgcttct tcatcgttca aacccggtgt gtgcttaacc ccatcggctg
     2101 gcattgtatc agcatgaagg cggtttgggt gctcaatata tagccggaat cgtgctgccg
     2161 gcttgaccca acgcggaaca gatatgtcaa gcggggcgca attataaaat gactcaatat
     2221 tcgctgcatc gtagattgcc gccccatttg ccgttaccag cggcgtagtc gaacttgcat
     2281 tccggggaca cgtttacgat ggacttcacc cgccttgaaa tcaaccgccg catggcgctg
     2341 ctcacttcgg ccgcgattgc cgcaaacgtc atcgatccca tgcgggcctt tgctcaggag
     2401 acgccccgca aaggcggtgt gttcaacgtc cattatggcg ccgagcaacg ccagctcaat
     2461 ccgagcctgc aggcgtccac cggtgtctac atcatcgggg gcaagatcca ggagaacctg
     2521 gtggatctcg acagcgaagc caagccggtc ggcgtgctcg ccgagagttg ggaagcttcc
     2581 cccgacggca tgaccgtcac cttcaagctg cgcaagggca tcacctggca cgacggcaag
     2641 cccttcacct cagccgacgt cgcgttcacg gcgatgaaca tgtggaagaa gatcctgaac
     2701 tacggctcga cgctgcaact ctttctcacc tcggtcgata cgcccgaccc gcagactgcg
     2761 atctttcgct acgagcgtcc gatgccgctg aacctgttgc tgcgcgcgct gcccgacctt
     2821 ggctatgtct ctgcgaaaca tctctacgag agcggcgaca tccgccagaa cccgcacaat
     2881 ctggcgccgg tcggcacggg cccgttcaaa ttcgttcaat atgagcgcgg gcaatatgtc
     2941 atcgccgagc gcaatccgga ttactggcgg ccaaattccc cctatctcga ccgcatcgtc
     3001 tggcgcgtga tcaccgaccg cgctgccgcg gcggcccaga tggaggccgg cgagctgcac
     3061 tatagtccgt tctcaggcct caccatttcc gacatggcgc ggcttgccaa ggacaagcgc
     3121 tttgttgtct cgaccaaggg caatgaaggc aacgcgcgga ccaacaccat cgagttcaat
     3181 ttccgccgca aggagctgtc cgatatccgc gtccgtcgcg ccatcgcgca cgcgatcaac
     3241 gtgcccttct tcatcgataa ttttttgggc gacttcgcaa agctcggaac cgggccgatc
     3301 ccttcgacct cgaccgattt ctatccgggg cccgacacgc cgcagtaccc ttacgacaag
     3361 gccaaggcga tcgccctgct tgacgaagcc ggctacaagc cgggtgccgg cgggcagcgc
     3421 ttttcgctcc gcctgctgcc agcgccgtgg ggcgaggaca tttcgctgtg gtcgaccttc
     3481 atccagcaat cgctctcgga ggtcggcatt cccatcgaca tcgtgcgcaa tgacggcggc
     3541 ggcttcctga agcaggtcta tgacgagcac gccttcgacc ttgccaccgg ctggcatcaa
     3601 taccgcaacg atcctgcggt ctcgacaacg gtctggtatc gttccggcca acccaagggc
     3661 gcgccctgga ccaaccagtg gggctgggaa gacgccaccg tggacagaac gatcgacgac
     3721 gccgcgaccg agatcgatcc gatcaagcgc aaggcgctct atgcccggtt cgtcaaggag
     3781 gtgaacacgg agctgccggt ctggatgccg atcgagcaga ttttcgtcac ggtcatcacg
     3841 gcaaaagcgc gcagccattc caacaccccg cgctggggct cgacaagctg gcatgatctc
     3901 tggctttctg cttagagcat catccggaaa agaggacacc ggctttctgc tcaaaaaata
     3961 gagcgggagg gcgaaagcca tcgcgcttta tcccgatttc agtctagatg aacccatgcg
     4021 catcttgagc cttgcggggc ggcgcctcgc cgcctcgatt ccgacattga tcctgatcct
     4081 gatcggcgta ttcctgctgc tgcaattcgc gcctggcgat actgtcgacg cgatgatggc
     4141 gcagatgggt ggcggggacg ctgcgaccgc gcgggaattg cgacatttct acgggctcga
     4201 tctctccatt cccgtgcagc tcggcaatta tctctggcgc ctggtgcggc tcgatctcgg
     4261 cttctccgcg atctacggca aaccggtcgc aaccgtgatc ctggaacggc tgccgccgac
     4321 cattctcttg atgacggcgg cactttcgtt tgcgttcttc ttcggtcttt tgttcggcgt
     4381 cgcggccgcg cgcggcgtca acaagtggcc ggatacgctg atctcgacgc tgggcctgat
     4441 cttctacgca acgccatcat tctggttcgg attgatggcg atcgtggtgt tctccgttta
     4501 tctgcagtgg ctgccgccgg gcggtttcga ggatatcgga aacgtgcaga ccggcatctg
     4561 gcgcgtgatc gacatcgcgc gccatctgat cctgccggca ttgacgctcg ggctgatctt
     4621 cctggcgatc tatctgcgca tcatgcgtgc ctcgatgctc gaggtgctca atctcgacta
     4681 tgtcaggacc gcccgcgcca agggcctcga cgaaaccgca gtgatcacac gacatgtcct
     4741 gcgcaatgcc ttgctgccga tggtcaccct gatcggtttg caagccggca ccatgctggg
     4801 cggctcggtc gtcgtcgaga gcgtgttctc gctgcccggt ctcggacggc ttgcctatga
     4861 atccgtggtc cagcgcgatc tcaacacgct tttgggaatc gtgttcgtgt cggcgctgct
     4921 cgtcatcgcg gtcaatttta ttgtcgatct cctctacgcg cgtctcgatc cgcgcatcac
     4981 ggtagacggc tgatggacgc ggccaggcgc tacttccgca gtcccgccgc cgttgcaggc
     5041 ctgatcctgc tgctcgtcgt catcgcgatg gcgatcagcg caacctggct ctatccgcgc
     5101 gatccgctgt cgcttgccgg acgtcccctg ctctggccgt tttccaatcc gcggtttccg
     5161 ctcgggacgg acaattcagg ccgcgatatc gcggcacaga ttttctacgg cgcgcggatt
     5221 tcgcttctga tcggcggcgt ggccacggcg attgcgattg cgatcggaat cctggtcgga
     5281 gcttttgccg gctactatgg cggccgggtc gacaacgtct tgatgcggat caccgaggcg
     5341 tttcagacat tgccgaactt tatccttctc ctggtgctgg tcgcggtctt cggctcaacg
     5401 ctgaccacgg tgaccatcgc agtcggggtg gtcgcctggc cggcaccggc gcggctgacc
     5461 cgcgcggagt ttctatcgtt gcgcaaccgc gaattcgtcc aggctggccg cacgctcggc
     5521 atgtctgata ttcagttgat cctcggggag atcctcccca acgccctgcc cccggtcgtg
     5581 gtctatgcca gcgtggtgat ggcggttgcg atcctgctcg agagtgcgct ggcctttctg
     5641 cacctctccg atcccaacgt cgcctcatgg ggaaacctga tcggactggg acgcgatgtg
     5701 ctgcgcgtgc agtggtatgt ctcggcgatt ccgggcgttg cgatcctggt caccgtcctt
     5761 gcggtctcgc tagtcggcca gggcctgaac gacgcgctca atccgaggct gaaggggcga
     5821 tgagcgagga cgcaccaatt ctgtcactgg agggcctcag tgtacggcta ccggccggcg
     5881 cagaccgctc gcatgcgctg tcgaacgttt ccctgtccgt cgccgccaac gaaatcctct
     5941 gcgtggtcgg cgagtccggt tccggcaagt cggtaatggc caatgccatc atgcgcctgc
     6001 tgccaccgga ggtcaggatc agcggcggac gcatggtgtt cgagggacgt gaccttgcac
     6061 aggctacggc tgccgcgatg cgcgcggtgc gcggcgaaca catttcaatg atttttcagg
     6121 aaccgatgac ggcgctcaat ccgctgcgca ccatcggcga ccagatcgcg gagatgttct
     6181 ccgtccacac ggacctgtcg aaagctgata tcagggcgaa agtgctctcg ctgctcgccg
     6241 acgtgagaat cgcggagcct gcggcagcgg ccaaggccta tccgcacgaa ctgtcaggcg
     6301 gtcagcgtca gcgcgcgatg atcgcgatgg cgcttgcgct ccatccaaag cttttgatcg
     6361 ccgacgagcc gacgacggcg ctcgatgtga ccacgcaggc ccagatcctg aaacttatcc
     6421 gcgatctgca gcaacgcagg aacaccgcgg tactgttcat cacccacgat ttcggcgtgg
     6481 tcgcagaaat cgccgaccga gtcgtcgtga tgcaacgggg catgatcgtc gaacagggat
     6541 cagcctcttc ggtactcaac aatccccaac atgcctatac gagacagctc atcgccgcgg
     6601 ttccgccgct gaaggcgccg ccaccgcgca agctcgccgc ggaagacatc ctgaccatgt
     6661 cgggtctgtc caagacctat cgaaccggcg gcttcctcgg ccgcggcgcg cgcgtcacag
     6721 ccgcagtcaa ggacgtgtcg ctcaacttgc cgaggggtgc gacgttggga atcgtaggcg
     6781 aatccggctc cggcaagtcg acgctcgcgc gctgcattgt acggctgatc gatccggaca
     6841 acggcgcaat catgctcgac ggcaaggatt gggccgcatt gtcgcgcaag gacgttcgta
     6901 gggaaacacg ccatatccag atggtatttc aggacccctt cgcctcgctc aacccgcgcc
     6961 gcagggcggc ggaactcgtg gcgcagggtc cgatcgtgca tggcacgccg cgcaccgagg
     7021 cgatcgccga ggccaggaat cttttcacac tggtcggtct tgacccttct gcaggcgacc
     7081 gcttcccgca cgagttttca ggcggccagc gccaacgcat cgggcttgcg cgtgcgcttg
     7141 cactcaagcc ggatgttctg gtggcggacg agccggtttc ggcgctggac gtctcggtgc
     7201 aagcgcaggt gctgaggttg ctgagcgagc tgcgcgagcg cttcgggctc tctatcatct
     7261 ttatcaccca cgaccttcgc gtcgccgcgc aaatctgcga tctcgtggcg gtcatgagag
     7321 acggtgcaat cgtggagcac ggacggacaa gcgacgtctt tggcaacccg caacatccct
     7381 atacgcaggc gttgctcgct tcgatcccgg gcggcgactt cacgcgcagg cgggacgccg
     7441 cgaatgcatg atccttcctg tcatgcgaag ttgctgaata tttaggccaa agacacgccc
     7501 tgaccaaaaa gattgctcgc ctcttcgtga agccgcgcag cgtaataatc agcacgacct
     7561 gtcgttatct caatagccgc tcgcccccac gcatttggct tagcggcggc gcgcatagaa
     7621 atgccggaga aagaacgatg agaaaaacca tcctgacaat tttcgcttcg gccctgctgg
     7681 ctggaaccat gatcgaggcg gccacggccg ccgagcatca caacgtcaga agagcaaccc
     7741 gcacagcgcc ggtcgtggtc ggcgagccgt tccgcgattc ctacgcttac gatcgcaacg
     7801 cctatggctc gtcatatccc gactattcct attggacatc gcaccttgaa ggcggcgcaa
     7861 tctccgcgcc ggccggccac tgaacggcga gccctctcac gccatctcgc gggcatcgcg
     7921 ccgccggtag acgagcagca tcagcggcgc gagcctgcgt aacaagctcc gcgcaggaat
     7981 cggcatcggt tccgtgaagg gcagtgcgag gtcgcgttcc tcagcgccgt gcaccgcctt
     8041 cgccaattcg ccaccaagcg caatcgacag cgcgacggcg cggccattgc agccggtcca
     8101 gccataggcg tcggggccca gtcggtgcat gcgcggaaga aaatccgtag tcatgccgac
     8161 atagccgttc cagacgtagt cgaaggagac gtcgccgatc tgcggccata accgttgcag
     8221 ccgctcagtg acgcgggcct tgatccgctc ggccttgtta cccggtccga tcacggcacc
     8281 tcccgtcacc agccggttgc gcgcatcgta gcgggcgaaa tagagctcgc catgagtgtc
     8341 ggacatcgcc tgccggccgg ggatgatggt cttgcggaca ttgtcggaca agggttgcgt
     8401 cgccatctgc cacgacaaca ccggcaccac ttcgcgcgcg aggtcgggta tcagcgattt
     8461 cgaaaactcg tcgctatagg cgttggttgc caccaccaat gctttcccgc tgatctgtcc
     8521 caacgcagtc ttcaccagcc atttgccatc gcgccgggca atgcttgtcg ccggcgagcg
     8581 cgcgaagatg cgcgcgccct tctctaccac cacgcccgcc aagccgcgcg acagcgccag
     8641 cggattgata tggccaccgc ttctgttcca gaagccgccg aaccacgcgt ccgaaccgag
     8701 catgtcgcgg acctggtcgc gggacagcaa ttccacgggc gcaccgaatt tcgaccattg
     8761 ccgcacccgc cgctccgcga ttttgatccg gccgggcgaa tgcaccggct gcacccagcc
     8821 ggtctgttcc tgctcggctg gaatgttgta gcgccttgtc aggtcgaaca ggatggacgc
     8881 gctgtcgcgc aggagagcga cgaagcgttc gcctgcggca ccgtgctttg cgatgatgtc
     8941 ctcgggatcg ggacgtgaca gggtcgggat cacctggccg ttgttgcgcc ccgacgctcc
     9001 ccaccccggc tccatcgcct cgacaacggc aacatcgaga cccgcgtcac gcagatgcag
     9061 cgcggtggac agcccggtga agccgccgcc gatgacgacg acatcggctt cctgcgcgcc
     9121 ggctagttcg ggcaattccg gacccgccgg cgtgactgcg gcccataacg actccggcca
     9181 gtgcacattt gtgctgttca tcagcatcct ttccggctgg ccgcgagcca gcttgcgagc
     9241 ctctcactgt tctggttacc ttaagtgatg ctttaagccc aaaggaaata acagagtggc
     9301 actgaagaac gtcataggaa tagatcacgc cgtcgtcatg gtacgggacc tcgacaaggc
     9361 ggcggagaac tggaaacgcc tgggtttcac cgtctcgcct cgcgggaccc acagcgcgca
     9421 catgggatcg ggcaattaca cgatcatgct cgaccccgac tatatcgaat tgctcggcgt
     9481 gctt
//
LOCUS       DATLJT010001623         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2692371,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001623 DATLJT010000000
VERSION     DATLJT010001623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2692371"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VM910_37070"
     CDS             complement(<1..1658)
                     /locus_tag="VM910_37070"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HVI68156.1"
                     /translation="MARRRLLSDEQQAPFWAWASDERAIVRQYTLSPADLDLIAKKRS
                     ASNQLGFAVLMCGMRFPGRVLDVTETPPAAVLAFIADQVGVPPAQFATYRQRLTNRRE
                     HIAELMAILDCRAFDSDAYRELLALAVSLAQGTPRAERLVQSVIDEARSRHILLPTRR
                     AIDLLCHRARARSESLLQRALTTGLTDETKNGLDALLKIAPEMTTTRLTWLRNASQSP
                     APTNILGLIERVEFLRKLGIDRERQQAIPASAFDRIARGALKISAQHLAEAVAPRRHA
                     LLTAAALSLESGLTDATLLMFDKLMGSLSRKAERRSQEKAAKSARDLQEKLRALTGAC
                     TAMIRAREKHDDPFIAIERQMKMGWSQFVTFVTETEAIVAPDQTDPKAELLRRYATVR
                     KIAAPFLEAFTFKATPSAKPIIDALDVLKALYRTGQRPLPAKAPIRFIRRVWRSLVIQ
                     GGEIDRKAYELCVFFELRDRLRAGDIWVEGSRQYQDFESYLIPEPTFALLKAEGPLPV
                     AIDTDVESYLAGRRAGLDREIEEVAMLADQGKLDGVDLTGGELV"
     gene            1749..>2090
                     /locus_tag="VM910_37075"
     CDS             1749..>2090
                     /locus_tag="VM910_37075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI68157.1"
                     /translation="MGQRAALYCRVSTADQSCDRQERDLRAFAKRSGFEIVGVYKEKA
                     SGAKTDRIERKKVMALAQAREIEAILVTELSRWGRSTIDLVQTLQSLQAWDVSVLAVS
                     GLQFDLSTPHGK"
BASE COUNT          365 a          664 c          681 g          380 t
ORIGIN      
        1 atgaccagct ctccgccggt gaggtcgaca ccatcgagct tgccctgatc ggcgagcatc
       61 gccacctcct cgatttcgcg gtcgaggccc gcccggcggc cggcgagata gctttccacg
      121 tcggtgtcga tcgcgaccgg caatggcccc tcggccttga gcagcgcgaa ggtcggctcc
      181 ggaatcaggt agctctcgaa gtcctgatat tggcggctgc cttcgaccca gatatcgccg
      241 gcgcgcagcc gatcgcggag ctcgaagaag acgcacagtt cataggcctt gcggtcaatc
      301 tcgccaccct ggatgacaag agaccgccag acgcgccgaa tgaagcggat tggcgccttg
      361 gccggcagag gtcgctgccc cgttcggtaa agcgccttga gcacgtcgag cgcgtcgatg
      421 atcggctttg ctgacggtgt cgccttgaag gtgaacgctt cgaggaaggg cgccgcgatc
      481 ttgcgaacgg tcgcatagcg ccgcagcagc tcggccttgg ggtcggtttg gtcaggggca
      541 acaatcgctt ccgtctccgt cacgaaggtg acgaactggc tccagcccat tttcatctgc
      601 cgctcgatgg cgatgaaggg atcatcgtgt ttctcgcgcg cgcggatcat agcggtgcag
      661 gcgccggtga gcgcgcgcag cttctcctgc agatcacggg cggatttagc cgccttctcc
      721 tgactgcgcc gctcggcctt gcgggatagg ctgcccatca gcttgtcgaa catcagaagc
      781 gttgcgtcgg tcagcccact ctccagcgac aaagcggccg ccgtcagcaa cgcgtgccgg
      841 cgcggcgcta ccgcctcggc taaatgctgg gcagatatct tcagggcccc gcgcgcgatg
      901 cgatcgaacg cgctggccgg gatggcttgc tgtcgctcgc ggtcgatccc gagcttgcgc
      961 aggaactcga ccctctcgat caagccgagg atgttggtcg gtgccggcga ctggctcgca
     1021 ttgcgcaacc aggtgagccg cgttgtggtc atctctggtg cgatcttgag cagcgcatcg
     1081 agaccattct tcgtttcgtc cgtcaggccg gtcgtgagcg cccgctgcaa cagcgattcg
     1141 ctgcgtgcac gggcccgatg gcaaagaagg tcgatcgctc gccgtgtcgg caacaggatg
     1201 tgtcgggatc gtgcctcatc gatgacggac tgcaccagcc gctccgcgcg cggagtgccc
     1261 tgggccaacg acacggcaag agccagcagc tcgcggtagg catcgctgtc gaaggcgcgg
     1321 caatcgagga tcgccatcag ctcggcgata tgctcccgcc gatttgtcag gcgctgccga
     1381 taggtcgcga actgcgcggg cggcacgccg acctgatcgg cgatgaaagc cagcaccgct
     1441 gcgggcggtg tctcggtgac atcgagcacg cggcctggaa agcgcatgcc acacatgagc
     1501 acagcaaacc cgagttgatt ggacgccgac cgcttcttgg cgatgaggtc gagatcggcc
     1561 gggctcagcg tgtattgccg cacgatcgcc cgctcgtcgg aagcccacgc ccagaacggc
     1621 gcctgttgct cgtcggagag aagacggcgg cgtgccatcg gctgtccttt caaactagag
     1681 tatatttggt acgcattcga gccccggatt taccggggtt tcaagcgcct tcggccgagg
     1741 gggttcaagt gggacagcgt gcagccctct actgccgcgt ctccactgcc gatcaatcat
     1801 gcgatcggca ggaacgtgac ctgcgtgcct ttgccaagcg tagcggcttc gagatcgtcg
     1861 gcgtctacaa ggagaaagcc tccggtgcca agaccgaccg tatcgagcgc aagaaggtga
     1921 tggcgctcgc gcaggctcgc gagatcgaag ccatcctggt caccgagctg tcccgatggg
     1981 ggcgcagcac catcgacctc gtgcagacgc tgcaatcgct gcaggcgtgg gatgtgtccg
     2041 tcctcgccgt ctcaggcctg caattcgacc tgtcgacgcc gcacggcaag
//
LOCUS       DATLJT010001624         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1313380,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001624 DATLJT010000000
VERSION     DATLJT010001624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313380"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VM910_37080"
     CDS             complement(<1..177)
                     /locus_tag="VM910_37080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI68158.1"
                     /translation="MLIYALVAGVLFGLYFSLVGIGLNLVFGVMRIVNLAHGDVLMLG
                     AFVALGVVALAGIDP"
     gene            complement(380..1627)
                     /locus_tag="VM910_37085"
     CDS             complement(380..1627)
                     /locus_tag="VM910_37085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HVI68159.1"
                     /translation="MFKVTGAAVLASLVLSAQAFAADAPAEIKIGTLYASSGRFASIS
                     MPVHSALKLWVDQKNAEGGVYVKAFDKKLPIKLVAYDDQSNTATATTLYNQLITQDKV
                     DLLVADSGSVLTAPAVAIARDHKTFLFDQTGTGASFFSKDNPYIALMADPVSTVWPKP
                     VADFITHDGPALGIKKIAILYSTNEFTGTQANAFRKFVKDSGVPVEIVYDQGIPTETT
                     NYTVIINNINNTSPDAVIHFGYAPNDIAFLRNIQDVGVKFKMLFCIYPGLETELLEKN
                     VGEKGLEHVFTYVPPSELEYPVNFGMTMKEYKAAWDKTYPDGKIEFGFNAVAGYTTAL
                     VIEKTLSVATSLDQMELRRAVFSLSGELKTLDGTFALDETGGQVGELTPLGQLALDDQ
                     GKIKFISIYPHETATGKPVYPAP"
     gene            1815..2225
                     /locus_tag="VM910_37090"
     CDS             1815..2225
                     /locus_tag="VM910_37090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HVI68160.1"
                     /translation="MAEPARAKPKPTPETQHFWDGTKAGELRLQRCEACAHIYFPPRP
                     FCPSCASRKVSVFKASGRGKLYSYIIHHRPAPGFTPPYSIAVVELDEGPRMMTNIVDC
                     PQTPEALELDMRVEVKFEKLDDKITLPLFRPAKG"
     gene            2230..3369
                     /locus_tag="VM910_37095"
     CDS             2230..3369
                     /locus_tag="VM910_37095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HVI68161.1"
                     /translation="MRRNQVAIVGAAETTELGVIPNMSQIQLHADAALNAIADAGLKL
                     SDIDGFATAVETPQQICHYLGITPTWVDGTSVGGCSFMLHVRHAAAAIEAGLCKTVLI
                     THAESGKSNIGRTPRMIGADSLNGQFELPYGVTTPASMFPIPVLRYMKTHGITHEQIA
                     MVAVVQREWAAKNPRATMKEPITVADVLNSRMIAYPFRLLQCCLVTDGGGALILTSAD
                     RAKDFPRKPVYILGSGESVETPMVSQMQTFDSSRAFKVAGPLAFREAGITHKDVDHLM
                     IYDAFAHLPLFGLGDLGFMPHEETGKFIADGNTRPGGKLPLNTNGGGLSYMHSGMYGM
                     YALQESVRQMRGIAPAQVPNAKISVCHGVGGMFAASGTIIFTNER"
     gene            3396..>3629
                     /locus_tag="VM910_37100"
     CDS             3396..>3629
                     /locus_tag="VM910_37100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68162.1"
                     /translation="MAKSLEGKSIVVTGAGRGIGREIALLCAAEGAKVVVNDPGGSAD
                     GSGSSATPAEEVVEEIKKRGGTAVANFETVAEAI"
BASE COUNT          734 a         1113 c         1090 g          692 t
ORIGIN      
        1 gggatcgatg cctgcgagcg ccaccacgcc gagcgcgaca aaggcgccga gcatcaacac
       61 gtcgccatgc gcaaggttga cgatgcgcat gacaccgaac acgagattga ggccgattcc
      121 gaccaggctg aaatacagcc caaacaggac gccggcgacc aacgcataaa tcaacattca
      181 gggcccgccc ctcgtggagc ggatttgacg ttcgccccgc cgtaccgcga ttgtcggaca
      241 gcgaacgtca atccaaaact ccgctggcaa taacaatttg ccaatggtcc tttgattcca
      301 gcattcgcaa acgtgcctct ttaacggatg cgaatgtcag aaacggacca ttagcgtacc
      361 aattcccgcc gtcgccgcat cacggagccg gatagaccgg cttgccggtc gcggtctcgt
      421 gcgggtagat cgagatgaac ttgatcttgc cctggtcgtc gagcgcaagc tgtccgagcg
      481 gcgtcaattc gccgacctgc ccgccggtct cgtccagcgc gaaggtgccg tccagcgtct
      541 tgagctcccc tgacaacgaa aacaccgcgc gtcgcaactc catctggtca agggaggttg
      601 cgaccgacag cgtcttttcg atcacgagcg cggtggtgta gccggcgacc gcgttgaacc
      661 cgaactcgat cttgccgtca gggtacgtct tgtcccatgc ggctttgtac tccttcatgg
      721 tcatgccgaa attgactgga tattccagtt cggacggcgg cacgtaagta aagacgtgct
      781 caaggccctt ttcaccgacg ttcttctcga gcaactccgt ctcgagccct ggatagatgc
      841 agaacagcat cttgaacttg acgccgacgt cctggatgtt gcgcaggaag gcgatatcgt
      901 tcggcgcgta gccgaaatga atcaccgcgt cgggactggt gttgttgatg ttattgatga
      961 tgacggtgta gttcgtcgtt tcggtcggga taccctgatc gtagacgatc tcgaccggca
     1021 cacccgagtc tttgacaaac ttgcggaaag cgttggcctg tgtccccgtg aattcgttgg
     1081 tcgagtagag gatcgcgatc ttcttgatgc cgagcgcagg cccgtcatgg gtgatgaaat
     1141 ccgcaaccgg cttcggccaa acggtcgaga cggggtccgc catcagcgcg atatagggat
     1201 tgtcctttga gaagaagctt gcgccggtac cggtctgatc gaacaggaac gtcttgtggt
     1261 cgcgcgcgat cgcaactgca ggcgcggtca agaccgaacc ggaatcggcg accaggagat
     1321 cgaccttgtc ctgggtaatg agctggttat agagcgtggt cgcagtcgcc gtattgctct
     1381 gatcgtcata ggccacaagc ttgatcggca gcttcttgtc gaacgccttc acgtagacgc
     1441 cgccctcggc gttcttctga tcgacccaga gtttgagcgc gctatggacc ggcatcgaga
     1501 tcgaggcaaa ccgccccgac gatgcgtaga gcgtgccgat cttgatctcc gccggcgcgt
     1561 cggcagcaaa ggcctgcgcc gataatacca ggcttgcgag gacagccgca cccgttacct
     1621 tgaacatgga tccctccgga tttttctttg tctagcgcac cttctacgcg ccggatcaga
     1681 gggacacaag ccgcccggtc ttatccatta tgcgtgtggt ggaatatgcg ccatgcgtgc
     1741 gacgccgatg gaccgttgac gccggcccgt tcaatgtcgc atcaaggccg taacaggttt
     1801 ggaggaaacc cgacatggcc gaaccagcac gcgcaaaacc gaaaccgacg ccggaaaccc
     1861 agcatttctg ggatggcacc aaggcgggcg aattgcgtct gcagcgctgc gaggcctgcg
     1921 cgcatatcta ttttccgccg cgcccgttct gcccgtcctg cgcctcgcgc aaggtttccg
     1981 tgttcaaggc gagcggcagg ggcaagctct acagctacat cattcatcat cgcccggctc
     2041 ccggcttcac tccgccctat tcgatcgccg tggtcgaact cgacgaaggt ccgcggatga
     2101 tgaccaacat cgtcgactgc ccgcagacgc ccgaggcgct cgaactcgac atgagggtgg
     2161 aggtcaagtt cgaaaagctc gacgacaaga tcacccttcc cctgttccgt ccggcgaaag
     2221 ggtaacatga tgcgcaggaa ccaggtcgcc attgtcggcg cggccgagac cacagaactc
     2281 ggcgtcatcc cgaacatgtc gcagattcag ctccatgcgg atgcggcatt gaacgccatc
     2341 gcggacgccg gattgaaact gtccgacatc gacggtttcg ccaccgcagt cgagacgccg
     2401 cagcagatct gccattacct cggcatcact ccgacctggg tcgacggcac ctcggtcggc
     2461 ggctgctcct tcatgctgca tgtccgccac gccgccgccg cgatcgaggc cgggctctgc
     2521 aagaccgtgc tgatcacgca cgcggaaagc ggtaagtcga atatcggccg cacgccgcgc
     2581 atgatcggag ccgacagcct gaacggacag tttgagttgc cctacggtgt cacgacaccg
     2641 gcaagcatgt ttccgatccc ggtgctgcgc tacatgaaga cccacggcat cacccacgag
     2701 cagatcgcga tggttgcggt ggtgcagcgg gaatgggcgg cgaaaaatcc gcgcgccacg
     2761 atgaaggagc ctatcacggt cgccgacgtg ctgaactcgc gaatgatcgc ctacccgttc
     2821 cgcctgctgc agtgctgcct cgtcaccgac ggcggcgggg cgctgatcct gacttctgcc
     2881 gatcgcgcca aggattttcc gaggaagcct gtctacatcc tcggcagcgg cgagagcgtg
     2941 gagacgccga tggtcagcca gatgcagacc ttcgattcct ctcgcgcttt caaggtcgcg
     3001 gggccgctcg ccttccgcga agcgggcatt acccataagg atgtcgatca tctgatgatc
     3061 tacgacgctt ttgcgcacct gccgctgttc ggccttggcg atctcggctt catgccgcac
     3121 gaggagactg gcaaattcat cgccgacggc aataccaggc ccggtgggaa acttccgctc
     3181 aacaccaatg gcggtggcct ctcctacatg cattcgggca tgtacggcat gtacgcgctg
     3241 caggagagtg tgcggcagat gcgcgggatt gcgccggcgc aggtgccgaa tgccaagatt
     3301 tcggtgtgcc acggcgtagg cggcatgttc gcagcgtccg gcacgatcat ctttaccaac
     3361 gagcgctgaa gcgctgataa tcacgggaga gagctttggc caaatcactg gaaggcaaat
     3421 cgatcgtcgt caccggcgcg ggccgcggca ttgggcgcga gattgcgctg ctctgcgcag
     3481 ccgaaggggc caaagtcgtg gtcaacgatc ccggcggctc cgccgacggc tcgggctcga
     3541 gcgcaactcc ggccgaagaa gtggtcgagg agatcaagaa gcgcggcggt accgcggtcg
     3601 ccaatttcga gacggtcgcc gaagcgatc
//
LOCUS       DATLJT010001625         3520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1080741,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001625 DATLJT010000000
VERSION     DATLJT010001625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080741"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..875)
                     /locus_tag="VM910_37105"
     CDS             complement(105..875)
                     /locus_tag="VM910_37105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVI68163.1"
                     /translation="MISPRQIAFRNEYRSRILGWYDGYFHIFIIYAMGGAAFYIYVEH
                     IHDVTLLEWLTVPLTFFFTNIFEWAVHKFIMHRPVNIKGLRSIYERHTLNHHQFFTDE
                     EMRFRDHKDWRVTVFPPYALVAFILMSLPLAIILGNIFTSNVGWLFMCVTTGMYLVYE
                     FMHFCCHVEENRFVRNCPFVNSLRRHHTAHHNGRLMMEVNMNLTFPIADWLFGTSDLN
                     RGLLGHLFNGYDTRYLKQKLRGTPKRPDEAAAVPVGNY"
     gene            complement(977..1282)
                     /locus_tag="VM910_37110"
     CDS             complement(977..1282)
                     /locus_tag="VM910_37110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HVI68164.1"
                     /translation="MASTSKVYEGRRTIDGLVVTVDGNRLDEHYDVKRFTKFGFEWSY
                     EGASPQQLALAILVEHLGDKEHAIKLAEPFMKKVIANLDNDWRLTGGEIDAALTALQ"
     gene            complement(1325..2017)
                     /locus_tag="VM910_37115"
     CDS             complement(1325..2017)
                     /locus_tag="VM910_37115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI68165.1"
                     /translation="MTRHAPTSKVKIVSAPERPETLTEQAYKQIEELIVTLRLKPGHV
                     LSEQSLSASLGIGRTPIREALQKLALEGLVTILPRKGILVSEINPHSQLRVLEVRREL
                     ERLLSRASAERATEGEKERFLTIARGMEKAARTKNDIVFMRLDRELNQLIVEAAQNDY
                     AARAMRFLQGLSRRFWYMHYRMTADLPLCARLHANQALAIGRGDAKGAAQATDRLMDY
                     VESFTRATVQSR"
     gene            complement(2039..2977)
                     /gene="panB"
                     /locus_tag="VM910_37120"
     CDS             complement(2039..2977)
                     /gene="panB"
                     /locus_tag="VM910_37120"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVI68166.1"
                     /translation="MAKNEARARALSGPSGVAGKAAADGEKKREKRTITEIRESKRTG
                     EKMVYMSVPDYTSAKWAEMAGVDVAVVGDSLAMVAHGYPNTIPASMDMMIMHAQAVRR
                     GAPNTFVLGCMPYQSYNTIDRALVNATRFMQEALSDAVKPQGGKSQAHILRALVDAGI
                     PTASDIGLTPHTIAMFGGFKIQGRRAEAAMKILEDALAIEDAGCFMLEFEAVPAKIAK
                     LISEQLTIPTIGIGAGAGTDGQILLCYDLLGVFTDFKPKFTKRYAKLTEVAVAGVRQY
                     VEEVKQGVFPDDDHSYTVDDAEYEKFAAMVAKRRQV"
     gene            3212..>3520
                     /locus_tag="VM910_37125"
     CDS             3212..>3520
                     /locus_tag="VM910_37125"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HVI68167.1"
                     /translation="MAIYQSMQRVRFRSGGDYEQFKLIFAGVRHHLKTLPGFLHLTWW
                     THPDDPTWHNEVSFWTSFDALKDWHMNTYHKHAKQWAVRSGAIMEDIITNFELKSTRL
                     I"
BASE COUNT          697 a         1065 c         1025 g          733 t
ORIGIN      
        1 gcaacgatca gagttatgat cgacacgatg gctttaacgt cggccggcat gtcagcaacc
       61 cgcatcgtgg acgaggtgga actcgcgcgg catccagggc cgcactagta gtttccgacc
      121 ggaacggcgg ctgcctcatc gggacgcttg ggcgtgccgc gtaatttttg cttgaggtag
      181 cgcgtgtcat agccgttgaa gagatgaccc agcaggccgc ggttgagatc cgaggtcccg
      241 aacagccagt cggcgatcgg aaacgtgaga ttcatattga cctccatcat cagccgcccg
      301 ttgtggtgag cggtatggtg ccgccgcagc gaattgacga aggggcaatt gcgaacgaac
      361 cggttttcct cgacgtggca gcagaaatgc atgaactcgt agacgaggta catgccggtc
      421 gtcacgcaca taaaaagcca accgacattc gaggtaaaaa tgttgccgag aataatggcc
      481 agcggcagag acatgaggat gaacgccacg agcgcgtagg gcggaaagac ggtcacccgc
      541 cagtctttgt gatcgcgaaa ccgcatttcc tcgtcggtga aaaactgatg atgattgagg
      601 gtgtgtcgtt cgtagatcga gcgcaggccc ttgatattga ccggccggtg catgatgaat
      661 ttgtggacgg cccactcgaa aatattcgtg aaaaaaaacg tcagtggcac ggtcagccat
      721 tccagcagcg tcacgtcgtg aatgtgctcg acgtagatgt aaaacgcggc gccgcccatc
      781 gcatagatga taaagatgtg gaaatagcca tcgtaccagc cgaggattcg ggaccgatat
      841 tcgttgcgga aggcgatctg gcgcgggctg atcatcgcgg gcctctcgga tccaccatct
      901 aatatatcac aagtctatca cccggccgac cccgcgttca aggcccaaaa tggtcgacca
      961 aacctggcca accccgtcat tgcagcgccg tgagcgccgc gtcgatttcg ccgccggtca
     1021 gacgccagtc gttgtctaga ttggcgatga ctttcttcat gaagggttca gcgagcttga
     1081 tggcgtgctc cttgtcgccc agatgttcga ccagaatcgc cagcgcaagc tgttgcgggc
     1141 tcgcaccctc atagctccac tcgaatccga acttcgtgaa ccgtttgacg tcgtaatgct
     1201 cgtccagacg attgccatcc acggttacaa ccaggccgtc gatggttcgt ctgccttcgt
     1261 agaccttcga tgtggaagcc atcagcgcga ttcccggcgg ctcgattccg tggcggtgta
     1321 atatttatcg ggattgtacg gtcgcacgcg tgaagctctc gacgtagtcc atcaagcgat
     1381 ccgttgcctg cgcggcgcct ttcgcatccc cgcggccgat cgccagcgcc tgattggcgt
     1441 gcagcctggc acaaaggggt aaatccgcgg tcatgcggta gtgcatgtac caaaaacgtc
     1501 gcgacagacc ttgcaggaac ctcatcgcgc gcgccgcata gtcgttttgc gccgcctcga
     1561 caatcagttg attgagctcc cggtcgagac gcatgaagac gatatcgttt ttcgtccgtg
     1621 cggccttttc cattccacgc gcgatggtca ggaagcgctc cttttctccc tccgtcgcgc
     1681 gctcggcgct cgcgcggctc agcagtcgtt caagctctcg ccgcacctcc agcacgcgaa
     1741 gctggctgtg cggattgatc tctgagacca gaattccctt gcgtggcagg atggtgacga
     1801 ggccctcgag cgcaagcttt tgcagcgctt cccggatcgg cgtgcgcccg atcccgagcg
     1861 atgccgaaag cgactgctcc gagaggacgt gcccgggctt cagccgcagc gtgacgatga
     1921 gttcctcgat ctgcttgtaa gcctgctcgg tcagagtttc cggtcgctcg ggagccgata
     1981 caatcttcac tttgctcgtg ggcgcatgcc tcgtcatcgc gacctcggca aacccgccct
     2041 aaacctgacg acgcttggcg accattgccg caaatttctc gtattcggcg tcgtcgaccg
     2101 tataggaatg atcgtcgtcg ggaaaaacgc cttgcttgac ttcctcgacg tattgcctga
     2161 cgcccgcgac cgcgacctcg gtcagcttgg catagcgttt ggtgaatttc ggcttgaaat
     2221 cggtaaacac gccgagcagg tcgtaacaga gaagaatctg gccgtcagtg cccgcgccgg
     2281 cgccgattcc gatcgtgggg atcgtcaact gttcggaaat cagcttggcg atcttcgccg
     2341 gcacagcttc gaattcgagc atgaaacatc cggcgtcctc gatcgcgagc gcatcctcca
     2401 gaatcttcat cgcggcctcg gcccggcgac cctggatctt aaatcccccg aacatcgcaa
     2461 tggtgtgagg cgtaaggccg atgtccgagg ccgtcggaat gccggcatcg acgagcgccc
     2521 gcaggatgtg cgcttgcgac ttaccgcctt gcggtttcac cgcgtccgag agcgcctcct
     2581 gcatgaagcg cgtggcattc accagcgcgc ggtcgatcgt gttgtagctc tggtaaggca
     2641 tgcatcccag aacgaaagtg ttcggggctc cgcgccgaac ggcctgagca tgcatgatca
     2701 tcatgtccat cgaggcggga atggtgtttg ggtatccgtg cgcgaccatc gcaagactgt
     2761 cgccgacgac cgcgacgtcg acccccgcca tttcggccca tttggccgag gtgtagtcgg
     2821 gcacggacat gtagaccatc ttttctccgg tcctcttcga ctcccggatt tccgtgatgg
     2881 tccgcttttc ccgctttttt tcgccgtcgg cggcggcttt gccggcaacg ccagacgggc
     2941 cggacaaagc gcgcgccctc gcttcgttct ttgccatggc cagcccgacg atcaaggtga
     3001 tctgatatac ttgtcgtatg ttagatagga tgcgtcgcgt tggtcaagcc gaggcggctt
     3061 ctccaggcgc atggcgccgc ccagggcagc agcgcgctgg ttccgtcaga ttttcgccga
     3121 ctggctcttt tgctccgccc gtcggcgccg aagattgcac gctatgcgcg accggccccc
     3181 cgcccgcgcc atccgtgggg agaacgatat catggccatc tatcagagca tgcaacgcgt
     3241 gagattcagg agcggcggcg actacgagca attcaagctc atcttcgcgg gcgtacggca
     3301 ccatctcaag accctgcccg gctttttgca cctgacctgg tggacgcacc ccgacgaccc
     3361 gacctggcac aatgaggtca gcttctggac cagcttcgac gcgctcaagg actggcacat
     3421 gaacacctac cacaagcacg ccaagcagtg ggcggtccgt tcaggtgcga tcatggaaga
     3481 catcatcacc aatttcgagc tcaagagcac ccgccttatc
//
LOCUS       DATLJT010001626         3460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1774124,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001626 DATLJT010000000
VERSION     DATLJT010001626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774124"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..933
                     /locus_tag="VM910_37130"
     CDS             238..933
                     /locus_tag="VM910_37130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HVI68168.1"
                     /translation="MALQLYELVGLDAERPFSPFCWRTRMAVAHKGLDCGSIPWRFTE
                     KSTIAPYGSNKVPVLLHDERAVADSWTIANYLEDTFPDHPSLFGGVGGRSAGRLINSW
                     GDIAVIGGLFSLIVADIPAHLGPVDAEYFRKSRGARLGKTLEEAVSARDKSVIAFRRS
                     LEPLRLTLKSQPYLGGTTPNYADYIVFGGFQWARVVSPFRVLEDSDPIYAWRERLLDA
                     FDGLARKSPGYPL"
     gene            complement(969..1652)
                     /locus_tag="VM910_37135"
     CDS             complement(969..1652)
                     /locus_tag="VM910_37135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVI68169.1"
                     /translation="MTGKLNFEAVLLDMDGTLLDTERVYFKALVGALAAHGFTDDVGA
                     LCHAMVGLPGPECEAMLIARYGEAFPLAELSRSYAVKRDEILKAGLPLKHGAIELLNA
                     LQEAGFPMAIVTSSSRQTTEAYLTLAGIRGRFRTMLTRDDVARGKPHPDLYLAAAEQL
                     RMKPHLCVAVEDSNPGVASALAAGAITIMVPDFVPPSPESRERCVAVLPDLIAVLEKL
                     QLDIGGTHR"
     gene            complement(1649..1840)
                     /locus_tag="VM910_37140"
     CDS             complement(1649..1840)
                     /locus_tag="VM910_37140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68170.1"
                     /translation="MTNWTESQATRRINCSRCGAELTCNPTGPCWCAEENLRLPMPLA
                     GEDCLCRECLRKTVENTAK"
     gene            1997..>3460
                     /locus_tag="VM910_37145"
     CDS             1997..>3460
                     /locus_tag="VM910_37145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVI68171.1"
                     /translation="MARDSLTALVALNRFGFGARGGASGDFVSAASDPRGFVKADLAR
                     PSGVLLEVPGLQSTPALARVLFEYQEEVKQAKAAQPASEAAQPAEQKPRRSLSLSTVA
                     TDVAGQTPAMKTADNGGTAAPGAPGEAMQPNTMQPDAPKPPQPPPQPLNVIQKTFRAE
                     ALARLQRAVLTDCGFTERLVVFWSNHFCISANKGGLARIWAGSFEREAIRPHALGRFA
                     DMLKAVEQHPAMLFFLDNQQSLGPDSRAGQNRKRGLNENLAREIMELHTLGVGGGYTQ
                     DDVTSLARVITGWTFVGRQGILGVPGSFVFNANAHQPGAQRVLGKIYEAGGVDQGEAV
                     LSDIARHPSTAKFVATKFARHFVADDPPPPLVARLQDVFTRTDGDLKALATALVDSDD
                     AWRAPVTKMRSPYEYLIAIGRLLARVPDDPGLYLNGLNVLGQPLWSPAGPNGFADTNA
                     AWAAPEGMKLRLDIAAQVASRVGNAIDPRDLLELAFAD"
BASE COUNT          624 a         1099 c         1096 g          641 t
ORIGIN      
        1 ctaccgagcg cgcctacgcc aaggcgaagg aggtcaatcc gaatttcggg cagcccgcga
       61 tccgcaccga ggaagagcgc aagatcctgt tcggacagac ctccgcggta gtgcgatagg
      121 gcgtattctt gcgcaacgtc aaggcctggc ttgtcccggc catccacgtt tcggtaagct
      181 cgaaatagcg tggatgcccg ctacaggcgc gggcatgacc agcggagcgc aacaatgatg
      241 gctctccaac tctacgaact ggtggggtta gatgccgagc gtccgttcag cccgttctgc
      301 tggcgaacgc gaatggcggt ggcacataag gggctcgatt gcggctcgat cccgtggcgc
      361 ttcactgaaa aaagtacgat cgcgccttac ggatcgaaca aggtacccgt cctgctgcac
      421 gacgagcggg cggtcgccga ttcctggacg attgcgaact atctcgagga cacctttccc
      481 gaccatccgt ctctgttcgg tggtgtgggc ggccgcagcg ctggtcgact gatcaattcg
      541 tggggcgata tcgccgtgat cggcggtctg ttttcgttga tcgttgccga catccctgcg
      601 catctcggac cggtcgatgc cgagtatttc cgcaagtcgc gcggggcgcg gttaggcaag
      661 actctggaag aggcggtgag cgcgcgcgac aaatccgtca tcgcgttccg cagatcgttg
      721 gagccgttgc ggctgacatt gaagagtcag ccgtatcttg gcggcacgac gccgaattat
      781 gccgattaca tcgtgttcgg cggatttcag tgggcacgcg tcgtcagccc gttcagggtg
      841 ctggaggaca gcgatcccat ttatgcctgg cgtgagcggc tgctcgacgc gttcgacggg
      901 ctcgctcgca agtctccggg ctatccgcta tagagaaaga gcaagccatg cagcgcggga
      961 ttcgtgtgtc agcggtgcgt accaccgatg tcaagctgca gcttttcgag cacggcgatc
     1021 agatcgggca gcactgcgac acatctttcg cgcgactccg ggctcggcgg cacgaagtcc
     1081 ggaaccatga tcgtgatagc gcccgcggca agagccgagg cgacgccagg attggagtcc
     1141 tcgaccgcga cgcagagatg cggcttcatc cgaagctgct cggcggcggc cagatataaa
     1201 tccggatgcg gcttgccgcg cgcgacgtcg tcgcgcgtca gcatggtccg gaaacggccg
     1261 cggatgccgg caagcgtcag ataggcctcc gtcgtttgcc gcgatgagga ggtgacgatc
     1321 gccatcggaa atccggcttc ctgcaacgca ttcaacaact cgatcgcgcc gtgcttcagg
     1381 ggcaaaccgg ctttcaggat ttcgtcgcgt ttgacggcat aggacctgct cagttcagca
     1441 agcggaaacg cctcgccata gcgcgcgatc agcatggcct cgcactccgg tcctggaagt
     1501 ccgaccatgg catggcagag cgcgccgacg tcgtcggtga agccgtgagc ggccagggcg
     1561 ccgacgaggg ccttgaaata gaccctttca gtgtcaagca gcgtgccgtc catgtcgagc
     1621 agcacggctt cgaaattcaa cttgcccgtc acttcgctgt attctccacc gtcttgcgca
     1681 agcattcccg gcacaggcaa tcctcgccgg caagcggcat cggcagccgg agattctctt
     1741 ccgcgcacca gcacggcccc gtcggattgc aggtcagttc cgctccgcag cgggagcagt
     1801 taatgcgtcg cgtggcttgg ctttctgtcc aatttgtcat gaagcaagaa gcagtttcga
     1861 cctgtttttc atgtcgggtt catctcgggc ggcggtatag tcgcccatca cgagtgcggc
     1921 agaactgaag agaactttcg aaccaccact actagcgtag actatcgcaa agcaaatcca
     1981 agccaaggat cattcaatgg cccgcgattc gctgaccgct ctggtggcgc tcaaccggtt
     2041 cggtttcggc gcgcgcggcg gcgcctccgg cgatttcgtc agtgcggcct ccgatcctcg
     2101 cggcttcgtc aaggccgacc tcgcccgacc gagcggcgta cttcttgagg tgcccgggct
     2161 gcagtcgacg cccgcgttgg cgcgggtctt gttcgaatat caggaggagg tcaagcaggc
     2221 gaaggcggca cagccagcat ccgaggccgc gcagcccgcc gagcagaagc cgcggcgctc
     2281 gctctcactc agcacggttg cgacggacgt tgccggacaa acgccggcga tgaagacggc
     2341 ggacaatggt ggcaccgcag caccgggtgc tccgggcgag gccatgcagc ccaacacgat
     2401 gcagcctgat gcgcccaagc cgccccaacc gcctccgcag ccgctcaacg tcatccagaa
     2461 gacgtttcgc gctgaggcgc tggcgcggct gcaacgcgcc gtgctcaccg attgcggctt
     2521 caccgagcgc ctcgtggtgt tctggtccaa tcatttctgc atttccgcca acaagggcgg
     2581 cctcgcgcgg atttgggccg gctccttcga gcgcgaggcg atcaggcctc atgcgctcgg
     2641 ccgcttcgcc gacatgctga aggcggtgga gcagcatccg gcgatgctgt tcttcctcga
     2701 caaccagcaa tcgctcgggc ccgactcgcg cgcgggccaa aaccgcaagc gcggccttaa
     2761 tgagaatctc gcccgcgaga tcatggaact gcatacgctg ggcgtcggtg gcggctacac
     2821 ccaggacgac gttacctcgc ttgcgcgcgt gatcaccggt tggaccttcg tcgggcgaca
     2881 gggaattctg ggcgtaccgg gcagcttcgt gttcaatgcc aatgcacatc agcccggcgc
     2941 ccagcgggtg ctcggcaaga tctacgaggc gggcggtgtt gaccagggcg aagcggtgct
     3001 ctccgacatc gcgcgccatc catcgactgc gaaattcgtc gccaccaaat tcgcgcgtca
     3061 tttcgtggcc gacgatccgc cgccgccgct ggtggcgcgg ctgcaggatg tcttcaccag
     3121 aaccgacggc gatctcaagg cgctcgccac cgcgctcgtc gattccgacg acgcctggcg
     3181 cgcgccggtc accaagatgc gctcgcctta cgaatatctg atcgcgatcg gacgcctgct
     3241 tgcgcgcgtg cccgacgatc ccggcctgta tctcaatggt ctcaacgtgc tcggtcagcc
     3301 tttgtggtcg ccggcgggcc cgaacggctt tgccgacacc aacgcggcat gggctgcgcc
     3361 cgagggcatg aagttgagac tggacatcgc ggcgcaggtc gcctcccgcg tcggcaacgc
     3421 gatcgatccg cgcgacttgc tcgagcttgc gtttgccgat
//
LOCUS       DATLJT010001627         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3842577,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001627 DATLJT010000000
VERSION     DATLJT010001627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842577"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1843
                     /locus_tag="VM910_37150"
     CDS             5..1843
                     /locus_tag="VM910_37150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68172.1"
                     /translation="MTVLSIIRDCVDALLHPSARHDALTRARHRAFMAPRLIGSLAAF
                     AAFPVYLAMRGAPGALEVCAFAWLIAPILLSWFLSRTGHYEGAHVLSSLALAGLVMAI
                     AASTGGIQSFAAVWLIVLPMEAALSASRRVVAVAATLAVSCVALLILFGHLGALPTPD
                     DQASVRNILMGFGVTSAALYAAGLAFGAESFARIGVSLLSVEEDRYRLLARNMSDVIS
                     RHRRNGAVQFISPAAEAMLGTPIARLLGHGLFDRVHVADRPAYLTALSDAARGGDARS
                     VEFRLRRDLPQVGNPVGQIDFIWVEMRCRPLEPGSQAAASAEAEVVAVMRDVTDRKMQ
                     EQALELARSAAEQADAAKSRFLATMSHELRTPLNAIIGFSEMMVREDALKLDAPRRRE
                     YAQLIHDSGQHLLSVVNDILDMSKMESGNFEISPEPFAPRAALINCCNLLALKARENG
                     VDLAMDAPKDLPVITGDPRAFKQIVLNLVSNAIKFTERGGKVTVAAAVEGSRLALRIT
                     DTGVGIAPDDLERIGDPFFQAGKTYERRHEGTGLGLSIVKSLVGLHAGEMVVQSKVGE
                     GTTIAVTLPLALSKPAASPPPSKIATLNPALRSTAPDQTVQVKKSA"
     gene            1836..2519
                     /locus_tag="VM910_37155"
     CDS             1836..2519
                     /locus_tag="VM910_37155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HVI68173.1"
                     /translation="MPRKSRDDDEMPRRRRGGASAAIDEERGLAMRILLHSPKDTVAA
                     LLAFAAGSAVIANALFLQKGPHPAPMFSSFVTPAAMPANPLPRPRPAEADASSSEPRL
                     AEPKFAEPRMIEPKAPDSLANLVKATTTPLSSTPMAPPPPPANVVRPPEPIPPAPIPI
                     TGSHRVAAVQRVLTEYGYGQLKPTGTVGPDTRAAIQKFERERKLPVTGQVSDRLVREL
                     SAVIGHPIE"
BASE COUNT          400 a          824 c          818 g          482 t
ORIGIN      
        1 ggccgtgact gtgttgagta tcatccgcga ttgtgtcgat gcgctgctgc atccgtcggc
       61 ccgtcatgac gcgctgacac gtgcgcgaca tcgtgccttt atggcgccgc gcctgatcgg
      121 cagccttgcg gcgttcgcgg cgttcccggt ctaccttgcg atgcgcggcg ctcccggcgc
      181 actggaggtc tgtgccttcg cctggctgat tgcgcctatt ctgctgtcct ggtttctgtc
      241 gcgcaccggc cactatgaag gcgcgcacgt gctgtcgtcg ctggcgctgg ccggtctcgt
      301 gatggcgatc gccgcaagca cgggcggcat ccaatccttt gccgcggtct ggcttatcgt
      361 gctcccgatg gaggcagctc tgtctgcctc gcgtcgtgtg gttgccgttg cggccaccct
      421 ggcggtgtcc tgtgtcgcgc ttctgattct gttcggccat ttgggtgcgt tgccgacgcc
      481 ggacgatcag gcgtcggtgc ggaatatact gatgggtttc ggggtcacgt ctgccgctct
      541 ctatgctgcc ggcctcgcgt tcggcgccga gtcgttcgcg cgcattggcg tctcgctgct
      601 ctccgtggag gaagatcgct accgcctgct ggcgcggaac atgagtgacg tgatctcgcg
      661 tcatcgcagg aacggcgcgg tgcagttcat ttcgcccgcg gcggaagcca tgctggggac
      721 gccgatcgct cggttgcttg ggcacggctt gttcgatcgc gtccatgtcg ccgatcggcc
      781 ggcttatctg actgcgctct ccgacgcggc gcgcggcggc gatgcgcgca gcgtcgagtt
      841 caggttgcgg cgggacctgc cgcaggttgg caatccggtc ggccagatcg acttcatctg
      901 ggtcgaaatg cgatgccggc cgctcgaacc gggatcgcag gcggcggcat ccgccgaagc
      961 cgaggtcgtc gcggtcatgc gcgacgtcac cgaccgcaag atgcaggagc aggctcttga
     1021 actcgcacgg agtgccgccg agcaggcgga cgccgccaag agccgcttcc ttgccaccat
     1081 gagtcacgag ttgcgcacac cgctgaacgc catcatcggc ttctccgaga tgatggtgcg
     1141 ggaagacgcg ctgaaactcg acgcgccgcg gcgcagggaa tatgcccagt tgatccacga
     1201 ttccggccag catctgttgt cggtcgtcaa cgacatcctc gacatgtcga aaatggaatc
     1261 cggcaatttc gaaatctcgc cggaaccgtt cgcgccgcgc gccgcgctga tcaattgttg
     1321 caacctgctt gcgctgaagg cgcgggagaa tggcgtcgat cttgcgatgg acgcgccgaa
     1381 ggatctgccg gtcatcaccg gcgacccgcg cgctttcaaa cagatcgtgc tcaacctggt
     1441 ctccaacgcc atcaaattca ccgagcgcgg cggcaaggtg acggttgcgg ctgccgtgga
     1501 gggatctcgg ctcgcgcttc gcatcaccga cacgggcgtc gggatcgcgc ccgacgacct
     1561 cgagcggatc ggtgatccct tcttccaggc gggcaagacc tacgagcgtc gccacgaagg
     1621 cactggcctc ggcctgtcaa tcgtgaaaag cctggtcggt ctgcatgccg gcgagatggt
     1681 cgtgcaaagc aaggtcggcg aaggaacgac gattgccgtg acattgccgc tcgctctgtc
     1741 caaaccggcg gcctcgccac caccgagcaa gatcgcaaca ctgaacccgg ctttgcggtc
     1801 gaccgcgccg gaccagacag ttcaggtgaa gaagagtgcc tagaaagagc agagacgatg
     1861 acgagatgcc gcgccgccgc cgtggcggcg cctcggctgc gatcgacgag gagcgcggtc
     1921 ttgcgatgcg catcctgctg cactctccga aggacacggt tgccgctttg cttgcttttg
     1981 cagccggcag cgccgtgatc gccaatgcgt tgttcctgca gaaggggccg catccggcac
     2041 cgatgttttc gtctttcgtc acgcctgctg ccatgccggc caatccattg ccgcggccgc
     2101 gcccggcgga agccgatgcg tcgtcgtcgg agccgcggct cgccgagccc aaattcgccg
     2161 aacccaggat gatcgagccc aaggccccgg attccctggc taacctggtc aaggcaacga
     2221 caacgccttt gtcatcgacg ccgatggctc cgccgccgcc gccggcgaac gtggttcgac
     2281 cgccggagcc gattcctccg gcccccatcc cgatcaccgg ctctcaccgc gtagcagcag
     2341 ttcagcgggt gctcaccgag tatggctatg gccagttgaa gccgacgggc acggttggtc
     2401 cggacacccg cgccgcgatc cagaaatttg agcgcgaacg caagctaccg gtgacgggtc
     2461 aagtatccga ccgactggtg cgcgagcttt ctgccgtcat cggccatccc atcgagtagc
     2521 ggct
//
LOCUS       DATLJT010001628         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_850987,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001628 DATLJT010000000
VERSION     DATLJT010001628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850987"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..443)
                     /locus_tag="VM910_37160"
     CDS             complement(126..443)
                     /locus_tag="VM910_37160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HVI68174.1"
                     /translation="MEHVAQVVDPFVFRLSIFVLAVFVGYFVVWSVTPALHTPLMSVT
                     NAISSVIVVGALLAVGVGLVSSGSGWARGFGFVALIFACVNIFGGFLVTQRMLAMYKK
                     KAK"
     gene            complement(467..1597)
                     /locus_tag="VM910_37165"
     CDS             complement(467..1597)
                     /locus_tag="VM910_37165"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HVI68175.1"
                     /translation="MKIAVAKEIDPAEPRVAASPDTIKKFKALGVDVAVEPGAGIKSG
                     LPDSEFASAGATVSADAVKDADIVIKVKRPEASELGQYKRGALVIAIMDPYGNETALK
                     TMADAGVSAFAMELMPRITRAQVMDVLSSQANLAGYRSVIEAAEAFGRAFPMMMTAAG
                     TVPAAKLFVMGVGVAGLQAIATARRLGAIVTATDVRPAVKEQVESLGAKFIAVEDEEF
                     KQAETAGGYAKEMSKEYQAKQAALTADHIKKQDVVITTALIPGRPAPRLISLDMVKTM
                     KPGSVLVDLAVERGGNVEGVKNGETVEVDGVKIVGFPNLAGRVAASASGLYARNLLSF
                     IETLVDKSTKALKVNWDDELVKATALTKDGKVVHPNFQPKSS"
     gene            complement(1731..1883)
                     /locus_tag="VM910_37170"
     CDS             complement(1731..1883)
                     /locus_tag="VM910_37170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HVI68176.1"
                     /translation="MADHSEVAYTTADGNDYKAHEATYEGFIKLVKYGTSGVALILIL
                     MAIFLT"
     gene            complement(2060..>2436)
                     /locus_tag="VM910_37175"
     CDS             complement(2060..>2436)
                     /locus_tag="VM910_37175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVI68177.1"
                     /translation="KNGELTAEQIGQIWLSVQGESLGPAIEIKPGYENFWMYIPHFIH
                     SPFYVYAYAFGDCLVNSLYAVYEHAADGFADRYLDMLAAGGTKHYSELLQPFGLDAKD
                     PKFWDGGLSVISGMIDELETMG"
BASE COUNT          465 a          776 c          737 g          458 t
ORIGIN      
        1 ccgggctcga cagcccgcgc agcgacagga tgaacagcac acccgcgacg agataaagca
       61 gtgcagacag attggcgttc atctcaggtc ctcacttgtc cccgtcaccc tgaggtgcgt
      121 accgatcact ttgccttctt cttgtacatc gccagcatgc gctgggtgac aaggaagccg
      181 ccgaagatgt tcacgcaggc gaagatcagc gcgacaaagc cgaacccccg cgcccagccg
      241 ctgccgctcg aaaccaggcc gacaccgacg gcgagcagcg cgcccaccac gatcacggac
      301 gaaatcgcgt tggtcaccga catcaagggc gtgtgcagag ccggcgtcac cgaccacacc
      361 acgaaatagc cgacgaagac ggcaagaacg aagatcgaca gccggaacac aaagggatcg
      421 acaacttggg cgacatgctc catggcttct ctccttaggc ccggaattac gaagatttgg
      481 gctggaagtt cgggtggaca accttgccat ccttggtcag cgcggtcgct ttcaccagct
      541 cgtcgtccca gttgaccttg agcgccttgg tcgatttgtc gaccagtgtc tcgatgaacg
      601 acagcaggtt gcgcgcatag aggccggagg cggaggccgc gacgcggccg gcaaggttgg
      661 ggaagccgac gatcttcaca ccgtcgacct cgacggtttc gccgttcttc acaccctcga
      721 cattgccgcc gcgctcgacc gcaagatcga ccagcaccga gccaggcttc atcgtcttga
      781 ccatgtccag gctgatgagg cgcggtgccg gcctccccgg gatcagggcg gtcgtgatca
      841 cgacgtcctg cttcttgatg tgatcggcgg tgagcgccgc ctgcttggcc tgatactctt
      901 tcgacatttc cttggcgtag ccgccggcag tctcggcctg cttgaactct tcgtcctcga
      961 ccgcgataaa cttggcgccc aggctctcga cctgctcttt caccgccggc cgcacgtcgg
     1021 ttgcggtgac gatcgcgccg agccggcgcg cggtggcgat cgcctgcagg cctgcgacgc
     1081 cgacgcccat cacgaacagt tttgccgccg gcaccgtgcc ggcagcggtc atcatcatcg
     1141 gaaaggcgcg gccgaacgct tcggcggcct cgatcaccga gcgatagccg gcaaggttgg
     1201 cctggctcga gagcacgtcc atgacctgag cgcgggtgat gcgcggcatc aattccatcg
     1261 cgaaggcaga gaccccggca tcggccatcg tcttcagcgc ggtctcgtta ccgtaggggt
     1321 ccatgatcgc gatcaccagc gcgccgcgct tgtattggcc aagctctgac gcctcgggcc
     1381 gcttcacctt gatgacaatg tcggcatcct tgaccgcatc cgcgctgacc gttgctccgg
     1441 cagaggcaaa ttccgaatcc ggcaggccgg atttgatgcc ggcacccggc tcgaccgcga
     1501 catcaacacc caacgccttg aatttcttta tcgtgtcggg cgaagccgca acccgcggct
     1561 cggcgggatc gatttccttg gcgacggcaa tcttcatgag gcctccggcg gcggcgggtg
     1621 cgcacgcgat aagactagcg ctagtccgac atggatggat accggttttg cgacgagcgc
     1681 ttatgcaagt ttttccgtcg cagcgcgccg ttgcgtgcaa cggcgatcga tcaggtcagg
     1741 aagatcgcca tcaggatgag aatgagggcg acgcccgagg tgccatactt cacgagcttg
     1801 atgaagccct cataggttgc ctcatgggct ttgtagtcgt tgccgtcggc ggtcgtatag
     1861 gccacttcgc tatggtctgc cattgctgtc cccagtcgat agatcgttgc gaggaattag
     1921 cgcaaaccgg cagacagggc aacgctccga cgacacggtt gccgcggcct atccgctcat
     1981 ttgccgggcc tgtccttggc ttggcgggtc agcccctcct cgactacctc gcaggaagcg
     2041 cggggcggta cgggccgggt cagcccatgg tttccagttc gtcgatcatg cctgaaatga
     2101 ccgacaagcc accgtcccag aatttcgggt ccttggcatc cagcccgaac ggctgcaaca
     2161 gctcggaata gtgcttggtg ccgccggctg cgagcatgtc gagatagcgg tcggcaaatc
     2221 cgtcagcggc gtgctcatag accgcgtaga gcgagttcac caggcagtcg ccaaaagcgt
     2281 aggcatagac atagaagggc gaatggatga aatgcgggat gtacatccag aaattctcgt
     2341 agcccggctt gatctcgatc gccggcccga gactttcgcc ttgcacggac aaccagatct
     2401 ggccgatctg ctcggcggtc agttcgccgt tcttgc
//
LOCUS       DATLJT010001629         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2231806,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001629 DATLJT010000000
VERSION     DATLJT010001629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231806"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VM910_37180"
     CDS             complement(<1..633)
                     /locus_tag="VM910_37180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68178.1"
                     /translation="MDTQHRWPATFAGIVSVFVLGSAGVKAEDKYPSKPVRIVVSFSA
                     GGPTDTVARVMGAKMADLLGQQFVVENKVGAGGNIGADLVAKAPPDGYTLLMATVSTH
                     AINPGLYKNMPYDPVRDFAPIGQVGVTPTLLGAHPSIPATDVKSLVAVIKENPGKFTY
                     GSSGLGSILHLCGEQFKALAGGVNIVHVPYRGSAPMMSDLVGGQISMAFDA"
     gene            830..1921
                     /locus_tag="VM910_37185"
     CDS             830..1921
                     /locus_tag="VM910_37185"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HVI68179.1"
                     /translation="MASMQGLTLLFALLLVLLLAFLFTLLGSCVQSLSASAQTAQPAA
                     ADLAAYGGADRMEKLVAAAKKEGSVVVYSSAAMDDMAALIAAFEKKYGVKVRLWRASS
                     ENIVQRAVVEARGGRFDADVFETGATAMESMQRERLFQEIKTPALSALLPAAILPHRE
                     WIGTRFNIFVAAYNTGLIKDGLPKRYDDLLDRRWADKLGVEAEDSDWFGAVVSALGED
                     KGLKLFRDIVAANGISVRKGHTLLANLVVTGEVPLALTAYAYKAEQLRKSGAPIGWFV
                     IPPGVARFEGAGVARRASHPNAAILFFEFMLTDGQDILLARDFFPARANARSLPEGAL
                     LHFLDPAKGLDEYAKWSKQYRDVVTNPTH"
     gene            1960..>2445
                     /locus_tag="VM910_37190"
     CDS             1960..>2445
                     /locus_tag="VM910_37190"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI68180.1"
                     /translation="MGDKQHDPDKERVFVRALQGAYSLKDELARLRAMPRVIKGKDLK
                     LDGGPQHFSRHYVEPEDGRTQTLHIHLEEYAPGAKTQKHGHVNEAAFYILDGAGYEIH
                     DGTRYDWKAGDVAIVHNNCVHQHFNASAKEPARALVIKTKPMYLFMNMLFQHTVEKRP
                     KT"
BASE COUNT          469 a          748 c          788 g          440 t
ORIGIN      
        1 ggcgtcgaac gccatcgaaa tctggccgcc gaccaagtcc gacatcatcg gcgcagatcc
       61 gcgatagggc acatgcacga tgttgacgcc gcctgccagc gctttgaatt gctcgccgca
      121 cagatgcagg atcgagccaa ggccggagga gccgtaggtg aatttccccg gattttcctt
      181 gatcacggcg acgaggctct tgacgtcggt cgcgggaatc gaagggtgcg ctccgagcag
      241 cgtcggcgtc acgccgacct gaccgatcgg cgcgaaatcg cgcaccgggt cgtacggcat
      301 gttcttgtac agtcccggat tgatggcgtg ggtcgacacc gtcgccatca gcaaggtgta
      361 accgtcgggc ggcgcctttg cgaccagatc ggcgccgatg ttgccgccgg cgccgacctt
      421 gttctccacc acgaactgct gccccagcag gtccgccatt ttcgcgccca tcacgcgcgc
      481 gacggtgtcg gttgggccgc cggcggagaa ggaaacgacg atgcggaccg gtttggacgg
      541 gtacttgtcc tcggctttta cacctgcgct gccgagcacg aataccgaga caatgccggc
      601 aaaagtggcc ggccatcgat gctgcgtgtc catggcgttt ctcccagatt ttatgaacac
      661 gatagcatgt cgtcgaacgg ggatcatcaa gcgacttgca gtcttttaga cggcgggctt
      721 gactagcgac gcaggaaagc tgcggcgagt gccgaaggga tcgtgacgac gggagcggtg
      781 cggcgtgaga ccatctaggc aaaagcctac ggcatctcgg ccaaagccca tggcgtcgat
      841 gcaggggctc acgctcctgt ttgcgctctt gctcgtcctc ctgctcgctt tcctgttcac
      901 gctcttgggt tcgtgcgtac aaagcctgtc cgcatcggcg cagaccgctc aaccggccgc
      961 ggcggatttg gccgcctatg gcggcgcgga tcgtatggag aagctcgtcg cggcggcgaa
     1021 gaaggaaggc tccgtcgtcg tctacagctc ggcggcgatg gacgacatgg cggcgctcat
     1081 cgcggcattc gagaagaagt acggcgtcaa ggtccgcctg tggcgcgcca gctcggaaaa
     1141 catcgtgcaa cgcgcggtgg tcgaggcccg cggcggccgt ttcgatgcgg acgtgttcga
     1201 gaccggcgcc accgcgatgg aatcgatgca gcgcgaacgg ttgttccagg agatcaaaac
     1261 gcccgcgcta tccgcgctcc ttccggcggc gatcctgccg catcgggagt ggatcgggac
     1321 acgcttcaac attttcgtcg ccgcctacaa caccggcctc atcaaggacg ggctgccgaa
     1381 acgttacgat gatctcctcg atcgccgctg ggcggacaag ctcggcgtgg aggccgagga
     1441 cagcgactgg ttcggcgcgg tggtgagcgc cttgggtgag gacaagggcc tcaagctgtt
     1501 ccgcgacatc gtcgccgcca acggcatatc ggtacgcaag ggtcataccc tgctggcgaa
     1561 cctcgtggtc accggtgaag ttccgctcgc gctcacggcc tatgcctaca aggccgagca
     1621 gctgcgcaaa agcggcgcgc cgatcggctg gttcgtcatt ccgcccggcg tggcacgctt
     1681 cgagggcgcc ggcgtcgccc gccgcgcctc gcatccgaat gccgcaatcc tgttcttcga
     1741 attcatgctc accgatgggc aggatatctt gcttgctcgc gacttctttc cggctcgggc
     1801 gaacgcaaga tcgctgccgg agggtgcttt gctccacttt ctcgaccccg ccaaagggct
     1861 ggatgaatat gcgaaatgga gtaaacaata ccgcgatgtc gtcacgaatc cgacgcactg
     1921 agcgcgcgag tcttgagaca ggtcgcagag gaattatgca tgggtgataa gcagcacgat
     1981 ccggacaaag agcgcgtctt cgtgcgcgcg ctgcaagggg cctacagtct taaggacgag
     2041 cttgctaggc tgcgggcgat gccgcgggtg atcaagggca aggaccttaa gctcgacggc
     2101 ggtccgcagc atttcagccg gcattatgtg gagcccgagg acggccgcac ccagacgctg
     2161 cacattcacc ttgaggaata tgcgccgggc gcaaagactc agaagcacgg ccacgtgaac
     2221 gaggcggcct tctacattct cgatggcgcg ggctacgaga tccacgacgg cacgcgttac
     2281 gactggaagg ccggcgacgt cgccatcgtg cataacaatt gcgtgcacca gcacttcaac
     2341 gccagcgcga aggagccggc gcgggctttg gtcatcaaga ccaagccgat gtacctgttc
     2401 atgaacatgc tgttccagca cacggtggag aagcgcccga agacg
//
LOCUS       DATLJT010001630         1991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2921625,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001630 DATLJT010000000
VERSION     DATLJT010001630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921625"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VM910_37195"
     CDS             complement(<1..548)
                     /locus_tag="VM910_37195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI68181.1"
                     /translation="MSRIRKNPAGVDPELLVKDAAKLMPEPTRRLFLRGGASLGALAF
                     LTGCDIVDSASAEHVLMKISYFNDRAQAWLFNPNKLAPTYPDSAVRRPFPFNAYYPEE
                     DAPEVDKDDYKLEVGGMVDNKKSWTLDELYALPQETQITRLVCVEGWSAIGKWTGTPL
                     REFLRRVGADTRAKYVWFRCAEG"
     gene            complement(581..1216)
                     /locus_tag="VM910_37200"
     CDS             complement(581..1216)
                     /locus_tag="VM910_37200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVI68182.1"
                     /translation="MSIGATHDQALTPAAPAGAIIHPLWVRLTHWVNAVAMVVMIGSG
                     WEIYNASPLFPFVFPRGITLGGWLAGALLWHFAAMWLLAVNGLAYLVLGIATGRFRRK
                     LVPIRPSEVLADTKAAFTGKLVYDDLSVYNAVQKLLYLGVILAGIVIVLSGLAIWKPV
                     QLQELTAVFGGYDAARYVHFFAMAAIVGFFVLHVVMALLVPKSLRAMITGR"
     gene            1396..>1991
                     /locus_tag="VM910_37205"
     CDS             1396..>1991
                     /locus_tag="VM910_37205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVI68183.1"
                     /translation="MSKTGGGNFFEDFRIGQVIKHAVPRTITVGDVALYNGLFGPRFA
                     VQSSDVFARAIGYPCSPVDDLLVFHVVFGKTVPDISLNAVANLGYAACRVLAPVYPGD
                     TLNAESEVIGLKENSSRKTGIVFVRSTGCKPDGSKVLEYIRWVMVRKRDDAAPAPGDH
                     VPRLPEAVIPNTLGDACPRIDVAAYDFTLAGSPYRFGD"
BASE COUNT          383 a          625 c          616 g          367 t
ORIGIN      
        1 ccctcggcgc agcgaaacca gacatatttc gcgcgcgtat cggcgccgac gcgcctgagg
       61 aactcgcgca gcggcgtgcc ggtccattta ccgatcgcgc tccaaccttc cacgcagacg
      121 aggcgggtga tttgagtttc ctgcggcagt gcgtagagct cgtcgagagt ccacgatttc
      181 ttgttgtcga ccatgccgcc gacttcgagc ttgtaatcgt ccttgtcgac ttcaggcgca
      241 tcctcctccg gataataggc gttgaacgga aacggccgcc tgaccgcgct gtcgggatag
      301 gtcggcgcaa gtttgttcgg attgaacagc catgcctgcg cccggtcgtt gaaataggaa
      361 attttcatca gcacgtgttc cgccgacgcg ctgtcgacga tgtcgcagcc ggtgaggaaa
      421 gcgagcgcgc cgaggctggc gccgccccgt aggaacaggc ggcgcgtggg ttcgggcatg
      481 agtttcgccg catccttgac gaggagctca ggatcgacgc ccgctgggtt ttttcgaatg
      541 cgtgacatcc tcgtttgttc ctggactttg aacctcgccc ctatcggccg gtgatcatgg
      601 cgcgcagtga tttcggaacg agcaaagcca tgaccacatg gaggacgaaa aagccgacga
      661 tggccgccat ggcgaagaag tgcacgtagc gggcggcatc gtaaccgccg aacacggcgg
      721 tcagttcctg caattgcacc ggcttccaga tggcgaggcc cgacagcacg atgacgatgc
      781 cggccagaat gacgccgaga tacagtagct tctgcacggc gttgtagacc gagagatcgt
      841 cgtagacgag ctttccggtg aatgcggctt tggtatcggc caagacctcg ctggggcgga
      901 tcggaacgag cttgcgccgg aagcgcccag tcgcgatgcc aagcaccaga tacgcgagac
      961 cattcaccgc caggagccac attgccgcga aatgccaaag cagcgcgccc gcaagccagc
     1021 ctccgagcgt gattccgcgg ggaaacacga acgggaacag cggcgaggca ttgtagatct
     1081 cccaacccga gccgatcatg acgaccatgg ccacggcatt cacccaatgg gtaaggcgca
     1141 cccagagcgg atggatgatc gcgcccgccg gcgcggcggg cgtcaaggcc tgatcatggg
     1201 tggctccgat cgacatggcc cgccttttgc aattgagcag caaatgagct gcaattgagc
     1261 gacctatagc aaattcgcgc gggccggtcg tttcacgttc ccgtgccgcc gacgcccgca
     1321 tccgagctac gtgaaccaag gctggccgga cgcacggctc gatcggtccg gcccaacagc
     1381 gatctggtga gacatatgtc gaagaccggt ggcggcaatt tctttgaaga cttccgcatc
     1441 ggtcaggtga tcaagcacgc cgtccctcgc accatcacgg tcggagacgt ggccctctac
     1501 aatggcctgt tcggcccgcg ttttgccgtt cagtcctcgg atgtgttcgc acgcgcgatc
     1561 ggctatccgt gctcgccggt cgacgatctc ctcgtcttcc acgttgtctt cggcaagacc
     1621 gtaccggaca tctcgctcaa cgcggttgcc aatctcggct atgccgcctg ccgcgttctc
     1681 gcgccggtct atccaggcga cactctcaat gccgaatccg aggtcatcgg gctcaaggag
     1741 aattcgagcc gcaagactgg catcgtcttc gtgcgctcga ccgggtgcaa gccggacggc
     1801 agcaaggtgc tcgagtacat acgctgggtc atggtgcgaa agcgcgacga cgcggcgccg
     1861 gcgcccggcg atcatgtgcc gcggctgccg gaagcagtga tcccgaacac gctcggcgat
     1921 gcctgcccgc gcatcgacgt cgccgcctac gacttcacgc ttgcgggcag tccctatcgc
     1981 ttcggcgatt a
//
LOCUS       DATLJT010001631         1744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4070029,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001631 DATLJT010000000
VERSION     DATLJT010001631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4070029"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..377
                     /locus_tag="VM910_37210"
                     /pseudo
     CDS             267..377
                     /locus_tag="VM910_37210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            461..772
                     /locus_tag="VM910_37215"
     CDS             461..772
                     /locus_tag="VM910_37215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68184.1"
                     /translation="MDREAAQRVMAVLDEQEDQALAAQAVAAIAMRRVEPEPVVPQPR
                     HGDEQQKRRPRVEAAPYRISRRDERRHPVSDRARAPALTHPDGTSSSNTRGARRRRRT
                     T"
     gene            complement(830..>1744)
                     /locus_tag="VM910_37220"
     CDS             complement(830..>1744)
                     /locus_tag="VM910_37220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HVI68185.1"
                     /translation="LEPVRVYVGLRSADTPEIRAKLALEELKRVGGFERSVLIVVTPT
                     GTGWVDPAALDSVEYLHDGSVASVALQYSYLASWLSLLVEPGYGAHAARALFKEIYGY
                     WTTLPKDSRPRLYLHGLSLGAMNSEQSTDLIEVLGDPFDGALWSGPPYSSGLWRTLTD
                     RRNPESPAWLPRFRDGSFVRFMNQHGEYPYRSSEPNAGWGPMRVVYLQYASDPVTFYD
                     YRSLYREPDWMRSPRGPDVSPELRWYPVVTLLQLTLDMPMATTSPVGYGHVFAPAHYI
                     DAWIAVTDVHNWSPEEVERLKQYLSKKP"
BASE COUNT          335 a          533 c          579 g          297 t
ORIGIN      
        1 aaggacttta tggatcgtga agccgcgcag cgggtgatgg ccgtgctcga cgagcaggag
       61 gatcaggcgc tggccgctca agcggtcgcg gcaatcgcga tgcggcgcgt cgaaccgctc
      121 ggattgagaa aatattgatc cggcttgagt acagttgaaa agcccggcgc agcgcacctt
      181 gtagagcgtc ccgaatacgt ttgctttaaa tgccctgggc gctctaagtg tcgtttgtcg
      241 ctcacatctg cgcccgctgg tcgcgcgtgc gcatcctgtt gcgtgccgag gcggggtttg
      301 cccgcgaaca gctgaggtat tggtgccgcc acaacggcgt cgactacctc ttcggcttga
      361 ccaagaatgc gcgcctcatc gccgcgacgc cgccgaattg aacgctgcac ggcggcacgg
      421 cgcgcaaacc agcagaccgc cgcggcgctt caaggacttt atggatcgtg aagccgcgca
      481 gcgggtgatg gccgtgctcg acgagcagga ggatcaggcg ctggccgctc aagcggtcgc
      541 ggcaatcgcg atgcggcgcg tcgaaccgga gcccgtggtg ccgcagccgc ggcacggcga
      601 cgagcaacag aagcggcgcc cgcgagttga ggccgcgccg tacaggatct ctcgccgcga
      661 tgagcgacgt catccggttt ccgatcgagc gcgcgcgccc gccctgaccc atcctgatgg
      721 gacgtccagc agcaacacgc gcggcgcgcg gcggcggcgc cggacaacat gaccgacgaa
      781 gcgctgtcgc aaggcctcca tgacgggtga tcggaacagc ggtcgcgcct cacggcttct
      841 tggaaaggta ttgcttcagt cgctcgactt cttctggcga ccaattgtga acatccgtca
      901 ccgcgatcca tgcgtcgatg tagtgcgccg gggcgaagac atggccatag ccgaccggcg
      961 acgtggtcgc catgggcatg tccagggtca gttgaagaag agtcacaacc ggataccacc
     1021 gtaattccgg cgacacgtcc gggccgcgcg gcgagcgcat ccagtcgggt tcacgataga
     1081 ggctgcgata gtcgtaaaag gtcaccgggt cgctcgcgta ttggagatac acgacccgca
     1141 tcggccccca cccggcattc ggttccgagc ttcggtaagg atactctccg tgctgattca
     1201 tgaaccgcac gaacgatccg tcgcggaagc gggggagcca agcgggcgat tcggggttgc
     1261 gacggtcggt gagcgtgcgc cagaggccgc ttgaatacgg cgggccgctc cagagtgcgc
     1321 catcgaacgg gtcgccgagc acttcaatca ggtcggtgga ttgttccgag ttcatggcgc
     1381 caagactgag cccgtgcaga taaagtctcg gtcggctgtc cttggggagc gtggtccagt
     1441 atccgtagat ctctttgaaa agagcgcgcg cggcatgggc gccgtagccc ggctccacaa
     1501 gcagggacag ccaactggcg agatatgagt actgcagggc aacgctcgca acggagccgt
     1561 catgcagata ttcgacgctg tccaatgctg ccgggtcgac ccagccggtg ccggtgggcg
     1621 tcaccacgat aagcacggat cgctcgaaac cgccgacgcg cttcaattct tccaacgcaa
     1681 gtttggcgcg aatttccggc gtgtccgccg atcgcagtcc gacatagacg cgaacaggtt
     1741 cgag
//
LOCUS       DATLJT010001632         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_626084,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001632 DATLJT010000000
VERSION     DATLJT010001632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626084"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..666)
                     /gene="recR"
                     /locus_tag="VM910_37225"
     CDS             complement(61..666)
                     /gene="recR"
                     /locus_tag="VM910_37225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HVI68186.1"
                     /translation="MPAAVAGPEIERLIQLLARLPGLGPRSARRAALHLIKKREALMT
                     PLSAALQVAIDKIQVCKTCGNIDTQNPCTVCTDPRRDGSIIVVVADVADLWALERANA
                     TNGRYHVLGATLSPLDGVGPQDLTIDALVKRAHDAEVKEIILALNATVDGQTTAHYIT
                     DLLQDANVKVTRLAHGVPVGGELDYLDEGTLSAAMRQRTLF"
     gene            complement(670..996)
                     /locus_tag="VM910_37230"
     CDS             complement(670..996)
                     /locus_tag="VM910_37230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HVI68187.1"
                     /translation="MPDFLGMMKQAAQLQSKMQAMQEELGHVEVEGLAGGGLVAVRMT
                     AKMEVKGVKIDPSLIKAEEREILEDLLVTAFADARRKAETAMQEKMQSLAGGLGLPPG
                     LLGLGS"
     gene            complement(1016..2878)
                     /locus_tag="VM910_37235"
     CDS             complement(1016..2878)
                     /locus_tag="VM910_37235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HVI68188.1"
                     /translation="MTDAGAPPSKLDAGEQGGFDLGSPSDAAKPYRVLARKYRPSSFE
                     DLIGQEAVVRTVSNAFETGRIPQAWILTGVRGVGKTTTARILARALNYELPDGSVKAP
                     TIHMPVLGVHCRAIMESRHMDVLEMDAASHTGVDDVRQINDSVRYAPASARYKVYIID
                     EVHMLSTAAFNAFLKTLEEPPEHAKFVFATTEIRKVPVTVLSRCQRFDLRRVEADVLM
                     AHLSNIAKKEGVEIEPEALGIIARAAEGSVRDSLSLLDQAIAHAAGAVRADDVRQMLG
                     LADRARVIDLFESLVRGDIAGAFREFREQYDTGADPIVVLSDLAEFVNFVTRVKIVPA
                     TADNVAFSETERVRARDFAAKLSMRVLSRMWQMLLKGISEAQGATRPAAAAEMVLVRI
                     AYVADMPTPDEAIRMIEQNGGSAPVVAVTSPSRSVPAPTASASVSPQLRVPPAPRGSI
                     EANARPQMAAPVTDAQAAARRISSFPEIVALAAEKRDLLIKSALEAEVRLVRIEDGRL
                     EVALERNASRTLVNDLSRKLEQWTGKRWTVIVSNEAGQPTLREQNLVEKNQRERAAES
                     DPRVKEVLARFPGTKVIEVRKLAAEPPESDTGAIGLGDDDFGADGSSDGPDDDL"
     gene            complement(3036..3132)
                     /gene="ffs"
                     /locus_tag="VM910_37240"
     ncRNA           complement(3036..3132)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VM910_37240"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          557 a         1040 c          987 g          588 t
ORIGIN      
        1 caagcccagc gtctggcgaa tcctggtttc gtcatgttgc aaatgtccgt tttgggtgag
       61 ttagaagagc gtgcgctggc gcatcgccgc agatagcgta ccttcgtcca gataatcaag
      121 ctcgccgccg accggaactc catgagccaa tcgcgtcacc ttgacgttgg cgtcctgcag
      181 aaggtcggtg atgtaatgcg cggtggtctg cccgtcgacc gtggcattca gtgcgagaat
      241 gatctccttg acctcggcat catgggcgcg cttcaccagc gcgtcgatcg tgagatcctg
      301 cggacccacg ccatcgagcg gcgacagtgt cgcgcccagc acgtgatagc gcccgttggt
      361 cgcgtttgcg cgttccagcg cccagagatc ggcaacatcg gcgacgacga cgatgatgga
      421 accatcgcgg cgaggatcgg tacagaccgt acaaggattt tgcgtgtcga tattgccgca
      481 ggtcttgcag acctgaatct tgtcgatcgc aacctgcagt gccgcggaca gcggcgtcat
      541 cagcgcttcg cgcttcttga tcaggtgcaa cgcggcccgt cgcgccgagc gcgggccgag
      601 tcccggcaac cgcgccaaaa gctggatcaa acgttcgatc tcgggaccgg caaccgcagc
      661 aggcataatt tacgaaccga ggccgagcaa cccgggcggc aggccgagcc ctccggccag
      721 cgattgcatc ttctcctgca tggcggtttc cgccttgcgc cgcgcgtcgg cgaacgccgt
      781 gaccagcaga tcctcgagga tctcgcgctc ctctgccttt attagcgaag gatcgatctt
      841 gacgcccttg acctccattt ttgcggtcat gcgcacggca accaggccgc cgcccgcgag
      901 gccctcgacc tcgacatgac cgagttcctc ctgcatcgcc tgcatcttcg attgcagttg
      961 cgccgcctgc ttcatcatgc cgagaaaatc gggcatcgta gcgtcctttg atgttctaga
     1021 ggtcgtcgtc agggccgtca gaggagccgt cggcgccaaa atcatcgtcg cccaggccga
     1081 tggcgccagt atcggattcc ggcggctccg cggcaagctt gcgcacctcg atcaccttgg
     1141 tgccgggaaa ccgcgcgagc acttccttga cccgcggatc cgattcggcg gcccgctcgc
     1201 gctggttctt ctcgaccagg ttctgctcgc gcagcgtcgg ctgaccggcc tcgttggaga
     1261 cgatcaccgt ccagcgcttg cccgtccatt gctcgagctt gcgcgagagg tcgttgacca
     1321 gcgtgcgcga ggcattgcgc tcgagcgcga cttccagccg tccgtcctcg atacgcacca
     1381 ggcggacttc ggcctcgagg gcgctcttga tcagcagatc gcgtttctcg gcggcgagcg
     1441 ccacgatctc cggaaagctc gaaatccgcc gcgccgccgc ctgcgcatcc gttaccggcg
     1501 ctgccatctg cgggcgggcg ttggcctcaa tcgacccgcg cggcgcggga ggcacgcgca
     1561 actgtgggct gacactcgca gaagcggtcg gtgcgggcac gctgcgcgac gggctcgtca
     1621 cagcgacgac cggcgcagag ccgccattct gctcgatcat gcggatcgcc tcatccggcg
     1681 tgggcatgtc ggcgacatag gcgatccgca ccagcaccat ttcggccgca gccgccgggc
     1741 gcgtcgcgcc ctgtgcctcg ctgataccct tgagcaacat ctgccacatc cgcgacagca
     1801 cccgcatcga aagctttgcc gcgaaatccc gcgcgcggac ccgctcggtc tcgctgaagg
     1861 cgacattgtc ggccgttgcc ggtacgatct tcacgcgcgt cacgaagttg acgaactcgg
     1921 cgagatcgga aagcaccacg atcggatcgg cgccggtgtc gtattgctcg cggaactcgc
     1981 gaaaggcgcc ggcgatgtcg ccgcggacaa gcgactcgaa caaatcgatc acgcgtgcac
     2041 ggtcggcaag ccccagcatc tgccgtacgt cgtcggcgcg caccgcgccc gccgcatgcg
     2101 cgatcgcctg atcgagcaac gacagcgagt cgcggacgga accttcggcg gcgcgggcga
     2161 tgatgccgag cgcttccggc tcgatctcga cgccttcctt cttcgcgata ttcgaaagat
     2221 gcgccatcag cacgtcggcc tcgacccggc gcaaatcgaa ccgctggcag cgcgacagca
     2281 cggtgaccgg aactttgcgg atttcggtgg tggcaaacac gaatttggcg tgctctggcg
     2341 gctcctccag cgttttcagg aaagcgttga aggccgccgt cgagagcatg tggacttcgt
     2401 cgatgatgta gaccttgtag cgcgcgctgg ctggcgcata acgcacgctg tcattgatct
     2461 gacgcacgtc gtcgacgccg gtatgagatg cagcgtccat ctccaggacg tccatgtgcc
     2521 ggctttccat gatcgcgcga caatgcacgc cgaggacagg catgtgtatg gtcggcgcct
     2581 tcaccgatcc gtccggcaat tcgtagttca gtgcacgggc gaggatgcgc gcggtcgtgg
     2641 tcttgcccac gccgcgcacg ccggtcaaaa tccaggcctg cggaattcga cccgtctcga
     2701 aggcattcga gacggtgcgc accacggcct cctgaccgat caggtcctca aagcttgagg
     2761 gacggtattt gcgcgccagt acacgatagg gctttgcggc atcggacgga cttcccaaat
     2821 cgaagccgcc ctgctcgcca gcatccagct tggaaggggg ggcgccagcg tcggtcatcg
     2881 atcgatccgc aattctctat gtttcgggcc gggttcgatc gcttgcgcgt gctctgattg
     2941 caaaaccggt gcccactctt gcggaatacg cgccagatga tgagcctcgc ggagcgcgga
     3001 tcggcgctcg cagcgcgaat agctcgtaaa aacgggtagg agactgacga gcgacccgat
     3061 ccggacctcg ttagggctgc ttccttccgg atctgacccg gttggcgagt ggctcgtcca
     3121 ccgccaatct cccggtccct atttggggcc aaaaggggca gaaagcaagc gg
//
LOCUS       DATLJT010001633         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4300577,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001633 DATLJT010000000
VERSION     DATLJT010001633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4300577"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="VM910_37245"
     CDS             complement(<1..2251)
                     /locus_tag="VM910_37245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI68189.1"
                     /translation="MTALPHDTEADLRRRILELEQRLQSIVAQRDEAIERQTAAALVN
                     FRLQNELRATVDRQKAGAEILRTIAAAPTDAEQSLQRIAETTARFFNASSATIRISDG
                     NRWTRTIAVGRASERIVAAVPTDQLAIDGKNLPGKVYRENRQIHIPDLDKVDPSIADF
                     PGLAPARAGGTRTVSGTPLRRGHGAIGALIIHRDRLAPFTPDELALQQNFADQAVIAI
                     ENARLFNETNEALERQTATSDILKVIASSPSDAQPVFEAIANSANRLLGGFSSTVFLF
                     IDGMAHLGAFTPTTPEADEVLRSTFPRPVAEFAPIQMTQAGEVTQVPDTEDLTDDIKN
                     IARARGYRSMLFAPLMNKGVSIGFVAVTRVQPGHFADHHAQLLQTFADQAVIAIENVR
                     LFDEVQAKTRDLEEALRYQTGSANILRVIASSPTDVKPVLKAIVESACELCGAYDALV
                     RLKEGDGLAFGAHHGPLPVSLASVPITENSTAGLAVIGREPVHVHDLLSSDGERFPHA
                     QELARMHGERTILSVPLLREGESIGAIVLRRTEMQPFGSKQISLLQTFADQAVIAIDN
                     VRLFEQVQARTRELTQSLDDLRAAQDRLIQTEKLASLGQLTAGIAHEIKNPLNFVNNF
                     SALSVELTDELKDVLKQATLVDKIRTDVEELTGLLKDNLAKVVQHGKRADSIVKSMLL
                     HSREGSGEHRPTDVNALVDESLNLAYHGARAEKPQFNVTLMRNFDPEAGMIEVFPQEI
                     TRVLLNLISN"
     gene            complement(2408..>3455)
                     /locus_tag="VM910_37250"
     CDS             complement(2408..>3455)
                     /locus_tag="VM910_37250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HVI68190.1"
                     /translation="PVIYAGQGIHYARAWPQLKRLAERLAIPVTTSLGGKSSFPETHP
                     LSLGSGGLAVPRAVPKFLAEADVIFGIGCSFTETSFGIAMPKGKAIIHATLDPNHLNK
                     DVEAKIGLVGDAGLVLDALLEEIGKTVTTDRDTSAVAREIAASHKEWLEKWMPKLTSN
                     AAPLNPYRVLWDLQHTVDIRSTIITHDAGSPRDQLSPFWKPVEPLSYIGWGKTTQLGY
                     GLGLAMGAKLARPDKLCINVCGDAAIGFTGMDFETAVREKIPIMSILLNNFSMAIELK
                     VMPVSTEKYRSTDISGDYAAMARAFGGYGERITSAEDIVPAIRRGIQKTREGTPVLLE
                     FITSKETEVSRPGT"
BASE COUNT          596 a         1019 c         1143 g          697 t
ORIGIN      
        1 cgtttgagat caaattcagc agaacgcggg tgatttcctg cggaaacact tcgatcatgc
       61 ctgcctcggg atcgaaattt cgcatgagcg tgacgttgaa ttgcggtttt tcggcgcgcg
      121 cgccgtgata ggccaaattg aggctttcgt ccaccagcgc attgacatct gtcggacgat
      181 gttcgccgga accttcgcga gaatgcaaaa gcatgctttt cacgatggaa tcggcgcgtt
      241 tgccgtgttg cacgactttg gcgaggttgt ccttcaaaag gccggtcaat tcctcgacgt
      301 cggtacgaat tttgtcgacg agcgtcgcct gcttcaacac gtctttgagt tcgtcggtca
      361 attccaccga aagtgcagaa aaattattca cgaaattcag agggtttttg atttcgtgtg
      421 caatgcccgc ggtcaactgc ccgagcgagg cgagcttttc ggtctggatc agccggtcct
      481 gcgccgcgcg caggtcgtcg agcgattgcg tgagctcgcg ggtgcgggcc tgcacttgct
      541 cgaacaagcg cacgttgtcg attgcgatca cggcctggtc ggcgaaggtt tgcagcaggc
      601 tgatctgctt gctgccgaag ggttgcattt cggtgcggcg aagcacgatt gcgccgatgc
      661 tctcgccctc gcgcaacagc ggaacgctca agatggtgcg ctcgccgtgc atgcgcgcca
      721 attcctgagc atgcgggaac ctctcaccat cgctggaaag caggtcgtga acatggacgg
      781 gctctcgtcc gatcaccgca aggccagcgg tggaattctc ggtgattggc acactcgcga
      841 ggctcaccgg aagcggacca tgatgcgcgc cgaatgccag gccgtctccc tcctttagcc
      901 gtaccaacgc gtcataagcg ccgcaaagct cgcaggcgct ttcgacgatc gccttgagca
      961 cgggtttcac gtcggtcggc gaggaagcga tcacgcggag gatgttggcg ctgccggtct
     1021 gatatctcag cgcctcctcg agatcgcgcg tcttggcctg cacttcgtcg aacaggcgga
     1081 cgttttcgat cgcgatcacg gcctggtcgg caaacgtctg cagcagttgc gcgtgatgat
     1141 cggcaaagtg gcccggctga acgcgcgtta ctgcgacgaa accgatcgac acgcctttgt
     1201 tcatcagcgg cgcaaacagc atgctgcggt agccgcgcgc ccgcgcaatg ttcttgatgt
     1261 cgtccgtcaa gtcctcggtg tccggaactt gcgtgacttc gccggcctgc gtcatttgaa
     1321 tcggcgcgaa ttcggcgacc ggccgcggga aagtcgacct caagacttcg tcggcttccg
     1381 gagtggtggg cgtgaacgcg cccaggtgag ccatgccgtc gatgaaaagg aacacggtgc
     1441 tcgaaaaacc gccaagcaag cggttggcgc tgttggcgat cgcctcgaac accggttgcg
     1501 cgtcggacgg cgaactcgcg atcaccttca ggatgtctga tgtcgcggtc tgccgttcca
     1561 gggcttcatt ggtctcgttg aacagccgcg cgttctcgat ggcaatcacg gcctgatcgg
     1621 caaaattctg ctgcagcgcc agttcgtccg gcgtgaacgg ggcgaggcgg tcgcggtgaa
     1681 tgatcagcgc gccgatcgcg ccgtggccgc gccggagcgg cgtgccggac acggtgcggg
     1741 tgccgccggc gcgggccggc gcgaggccgg gaaagtcggc gatcgacggg tcgaccttat
     1801 cgaggtccgg aatgtggatc tggcggttct cgcgatagac cttgccggga aggttcttgc
     1861 cgtcgatcgc gagctgatcg gttggaacgg ccgcgacgat gcgctccgaa gcgcggccga
     1921 cggcgatcgt gcgcgtccac cgattgccgt cggagatgcg gatcgtggcg ctggaggcat
     1981 tgaagaaacg cgcggtggtc tcggcgatcc gctgcaagga ttgctcggcg tcggtgggtg
     2041 ccgccgcgat cgtgcgcagg atctcggcac cggccttctg gcgatcgacg gtcgcacgca
     2101 gctcgttttg cagccggaaa ttgaccagcg cggccgcggt ctgccgttcg atcgcctcgt
     2161 cgcgctgggc gacgatcgat tgcagccgct gctcaagctc caaaatcctg cgcctgagat
     2221 cggcttccgt gtcatgggga agcgcggtca ttccggtcaa atccaggtcg cccggaggac
     2281 gccgagcgtg ccgggctcaa atgttcacaa gccgttctgg gcgcacgatt attccattcc
     2341 ggcggctgtc aggccagcgc cggaggcgct tttttcggat cggggcgtta acgccgtcgg
     2401 ggcctgatca ggtccccggt cgcgaaacct cggtctcctt ggaggtgatg aattccagca
     2461 gcacaggcgt gccttcccgg gtcttctgga ttccgcgcct gatcgccggc acgatgtcct
     2521 ccgcgctcgt gatgcgctcg ccgtacccgc cgaaggcgcg cgccatggcg gcatagtcgc
     2581 cggagatgtc ggtcgaccga tatttctcgg tcgagacagg catcaccttc agctcgatcg
     2641 ccatcgagaa attgttgagc aggatggaca tgatcggaat tttctcgcgc accgcggtct
     2701 cgaaatccat tcccgtgaag ccgatcgcgg cgtcgccgca gacgttgatg cagagtttgt
     2761 cgggccttgc gagcttggcg cccatcgcaa gtcccagccc gtagccgagt tgggtggtct
     2821 tgccccagcc gatataggag agcggctcga ccggcttcca gaacggcgag agctggtcgc
     2881 gggggctgcc ggcgtcgtgg gtaatgatgg tgctgcggat gtcgacggtg tgctgaaggt
     2941 cccagagcac acggtaagga ttgagcggcg cggcattcga agtgagtttc ggcatccact
     3001 tttcgagcca ctccttgtgg gaagcggcga tctcgcgggc gactgcgctc gtgtcgcgat
     3061 ctgtcgtgac ggtcttgccg atctcttcca gcagcgcatc gagcacgagg ccggcgtcgc
     3121 cgaccaggcc gatcttcgct tctacgtcct tgttgaggtg attgggatca agcgtcgcat
     3181 ggatgatggc cttgcccttc ggcatggcga tgccgaacga ggtctcggtg aaggagcaac
     3241 cgatgccgaa gatgacgtcg gcctcggcaa ggaatttcgg cacagcgcgc ggcactgcca
     3301 atccgcccga gccgagcgac agcggatggg tctcgggaaa tgacgatttg ccgcccaggc
     3361 tcgtggtgac gggaatggcg agccgttcgg ccagccgctt caactgcggc caggcgcgcg
     3421 cgtagtgaat gccctggccg gcataaatca ccggg
//
LOCUS       DATLJT010001634         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_164555,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001634 DATLJT010000000
VERSION     DATLJT010001634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164555"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VM910_37255"
     CDS             <1..600
                     /locus_tag="VM910_37255"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HVI68191.1"
                     /translation="AVGQKIYVNGGAGIPQGMQLPDGLVGGGPVDLYSTLEVFDTDTN
                     TWTRLAPMPTPRNHHSIAYVDGKLYAIGGRVGSCYSGGWSSNVWMNEANDIATNTWAT
                     RAPMPTARSGTGIAVLDGKIYVLGGEGWIDDFGGVFRTNEVYDPKTNSWSRATRMITP
                     RHGFAVAQVGKAIYAVSGVNNAGGAGTLSVVNVNEIYEP"
     gene            753..1030
                     /locus_tag="VM910_37260"
                     /pseudo
     CDS             753..1030
                     /locus_tag="VM910_37260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508891.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            complement(1047..1463)
                     /locus_tag="VM910_37265"
     CDS             complement(1047..1463)
                     /locus_tag="VM910_37265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68192.1"
                     /translation="MRASDAPQATGIEVVATQPTKADRATLAMFESTERKTLPGAAYI
                     VKVRLKTLPEPTSRGFALYVGDLRIPKYWTYRQGIWFKVFDPQFFVEHKGEPIRFSLN
                     DTEFVKTRLKLTVPKMAKTKGGRTAARLPAQEDVLK"
     gene            complement(1498..>2109)
                     /locus_tag="VM910_37270"
     CDS             complement(1498..>2109)
                     /locus_tag="VM910_37270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68193.1"
                     /translation="VLRDHATAVAWMPAGATFVSQYQDPERVYLQHEQEAVAPPQISL
                     PVQGTAAADAMTATRVLKDLVNSFAPITTITLRLEWNAPGARLLLQVSPVTLPAERYK
                     VLALRVGQSTEPKNAPNRDQDFTIEVSSGSRTASFPGSSVHRLIYPDVVFGAGKIVMQ
                     TLRLPIASLVERGVDPNDIRSVEFLFDRRSSGTLYVGDVQVSN"
BASE COUNT          399 a          650 c          676 g          384 t
ORIGIN      
        1 gccgtcggcc agaagatata cgtgaacggt ggcgccggca ttccgcaggg tatgcagctt
       61 cccgatggcc tcgtcggcgg cggcccggtc gatctctaca gcacactcga agtgttcgat
      121 accgacacca atacgtggac aaggcttgca ccgatgccca ccccgcggaa tcaccacagt
      181 atcgcctacg tcgacggcaa gctctacgcc atcggtgggc gggtgggctc gtgctattcg
      241 ggcggttggt cgtcgaatgt gtggatgaac gaggccaatg acatcgccac caacacttgg
      301 gcgacgcgcg cgccgatgcc aacagcgcgc tcgggaaccg gcatcgccgt tctcgacgga
      361 aaaatttacg ttttgggtgg cgaaggttgg atcgacgatt ttggcggcgt gttccgaacg
      421 aacgaagtct acgatccgaa gaccaatagc tggtcgcgag cgacccgcat gatcacgcct
      481 cgccacggct tcgcggtcgc acaggttggc aaggccatct atgcggtgtc cggggtgaac
      541 aacgcgggcg gagccggcac gctctccgtt gtcaacgtca atgaaatcta cgagccctga
      601 caacgtcgcc tgttaagctg cctgcccgac caaagtctgc gagcacgctg acgtaccgcg
      661 tccgctcgct ctgttgcccg cgcgccgcga gcggccacgt tgcttccgcg ccgccgagcc
      721 gcgtgatgaa ctcgcgatgt ttcaattgat cgctatcgac ggcagctgag gacctgctgg
      781 ccgatgccac atggcaggac atcagctggc gcaccggaac gaaaggaaag ctcaaagctc
      841 gttttgctgc tgtccgcgtg cgggttgccg acggaccacc ggagcggatc agggacaagg
      901 gtcagcaaca tctgcccgag gacgaggctt ggctcatcgg cgaagacagg atgtcgggag
      961 agaagaaaac tatctcgcca acctgccggc gaacaccgac ctgcgcacct tagcggccac
     1021 catctcggcg gccgcgcgaa aacgcgctat ttgagcacgt cctcctgcgc tggcaatcgt
     1081 gccgcggtcc ggccgccctt cgtcttcgcc atcttcggca cggtaagctt gagccgcgtc
     1141 ttgacgaatt cggtgtcgtt cagcgagaaa cggattggct cgcccttgtg ttcgacgaaa
     1201 aactgcggat cgaacacctt gaaccagatg ccctggcggt aggtccagta cttcgggatt
     1261 cgcagatcgc cgacgtagag cgcaaagccg cgcgacgtcg gttcgggcaa ggtcttgagt
     1321 cgaaccttca cgatgtaggc agcgccgggc agcgtcttgc gctcggtgga ctcgaacatc
     1381 gccagcgtgg cacgatcggc tttggtgggt tgcgtggcga caacctcgat gccggtggcc
     1441 tgaggtgcgt cggaggctct catggcgcgg atcctcacat gctcgattca aattgcatca
     1501 attggatacc tggacatctc cgacgtacag cgtccccgac gaacgccgat cgaacaggaa
     1561 ctcgacgctg cggatatcgt tcgggtcgac gccgcgttcg accagcgacg caatcggcaa
     1621 gcgcagcgtc tgcatcacga tcttgccggc gccgaagacg acgtccggat agatgagccg
     1681 atggaccgag ctcccgggga atgacgcggt gcgtgatccg ctcgagactt cgatcgtgaa
     1741 gtcctggtcg cggttcgggg cgttcttagg ctcggtcgat tgtcccacgc gcagcgcgag
     1801 caccttgtag cgctccgccg gcaacgtcac aggtgaaacc tgcaagagca ggcgcgcccc
     1861 gggcgcgttc cattcgaggc gcagggtgat cgtcgtgatt ggcgcgaacg agttcaccag
     1921 atccttcagc acgcgcgttg cggtcatggc gtcggccgcg gccgttccct gtaccggcag
     1981 ggagatctgt ggcggcgcaa cggcttcctg ctcgtgctgc agatagacgc gttccggatc
     2041 ctggtattgc gacacgaacg tggcgcccgc gggcatccag gcgacggcgg tcgcgtggtc
     2101 gcgcaaaac
//
LOCUS       DATLJT010001635         5746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4530486,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001635 DATLJT010000000
VERSION     DATLJT010001635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4530486"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VM910_37275"
     CDS             <1..331
                     /locus_tag="VM910_37275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68194.1"
                     /translation="GRLKSWIGFRGGVLGRNLRVLVSLLGLAAPLMAQGGATPRDSGA
                     TLHIESRAVLVDVIVTDQHGNPVTGLKQDAFSVTEQGKPQPTAGSQSSRLATRCASTS
                     TWEAPTS"
     gene            244..528
                     /locus_tag="VM910_37280"
                     /pseudo
     CDS             244..528
                     /locus_tag="VM910_37280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
     gene            650..1381
                     /locus_tag="VM910_37285"
     CDS             650..1381
                     /locus_tag="VM910_37285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68195.1"
                     /translation="MSDRLKGKRAFVTAAAAGIGRACALAFAREGASVFATDIDETGL
                     AALINEGITEISKLDVRDSAAVNAIARRFGKVDILLNAAGFVHHGTVLDCSDEDFDFS
                     FDLNVKSMHRTIRAFLPAMLEGGGAIVNISSAAGVFKAAPNRYVYGATKAAVAALTRS
                     VAVDFISKGIRCNCICPGTIETPSMLQRAAAAGAGGREMFVSRQPMGRLGTAEEIASL
                     AIYLASDESAFTTGVAHVIDGGWTL"
     gene            1572..2318
                     /locus_tag="VM910_37290"
     CDS             1572..2318
                     /locus_tag="VM910_37290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68196.1"
                     /translation="MNKIDLNGRTAVVTGGAQGFGRAITERFVASGAKVAIWDHDQAL
                     AEKTAKEIGNAVSPFKVDVTAIGAVEKTRDATVSAFGKIDILVNNAGIAGVNKTVWET
                     DFEEWRKVLRINLDGPFICCKAIVPQMLKQKYGRIVNIASIAGKEGNPNAAHYSASKA
                     GLIALTKSLGKELATHDILVNAVTPAAAKTAIFDQMTKQHIDFMLSKIPKARFVLVEE
                     VAAMVAWLASEDCAFSTGAVFDISGGRATY"
     gene            2402..2737
                     /locus_tag="VM910_37295"
     CDS             2402..2737
                     /locus_tag="VM910_37295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVI68197.1"
                     /translation="MAKAPPSAKRDRIRNFTPALVDYTNDVLYGDLWERKGLSKRDRS
                     LITVAALVATYRPEQLVSHLTRAINNGVTQEEITEVITHMAFYAGWPAAMSAAQVAYT
                     VLVEGNKGD"
     gene            2741..3694
                     /locus_tag="VM910_37300"
     CDS             2741..3694
                     /locus_tag="VM910_37300"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007825113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68198.1"
                     /translation="MAEDIVVGFIGLGTMGGKMATNIQKAGYKVIVHDLHRQSASHHL
                     QAGAEWAGTPRALAEKSDVIFTSLPEPADVERVALGADGLIDGLKKGAAYFDLSTNSP
                     GVVKRIHDAFAAKGARMLDAPVSGGPSGAASRKMAIWVGGDKAAFDQYKAVLDAMGDR
                     PAYIGQIGSATVAKLVHNMSSYALACALAETFTMGVKAGMDPVALWEAVRQGVSGRRL
                     TFDGLLEQFLPGKYDPPNFALKLATKDVALATALGRELGVPMRICNLTYAEMIEACNR
                     GWEGRDSRAVMLLQQERAGVKVAADPERVKQAAERAKSGVT"
     gene            complement(3687..3986)
                     /locus_tag="VM910_37305"
     CDS             complement(3687..3986)
                     /locus_tag="VM910_37305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVI68199.1"
                     /translation="MRQRHGRTDGAALTFYLWAFALERTTPTRVAISVTVNPITASLF
                     GAVLLDEPLRWNLMVGIAAVFIGIFAATTAGRPRPKKGALFKIPDERSDSEVRIR"
     gene            4147..4827
                     /locus_tag="VM910_37310"
     CDS             4147..4827
                     /locus_tag="VM910_37310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVI68200.1"
                     /translation="MNISLYDISVWVLPLVIAITFHEAAHGFVAHQLGDDTAYKLGRV
                     SFNPLRHIDPFGTLVLPGMLLLSHSPFLFGYAKPVPVNFRALREPRVGMVLVALAGPA
                     TNILLALVAAASFHILGFVPANAAQWLAENLKNAVVINVVLAVFNMMPIPPLDGGRVA
                     VGLLPRPLAIPLARLEPFGMLILIGFLILLPLAGSQFGLNLDVISAILRRSTDLIIRF
                     ILVLTGNA"
     gene            4876..5286
                     /locus_tag="VM910_37315"
     CDS             4876..5286
                     /locus_tag="VM910_37315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68201.1"
                     /translation="MLIARRADTRARADFATWKMIAKLNGASSLPAEAQNFLAGYRTL
                     LAKMNEADATEATIRLMYKTYYSEMGGGGSPPEIRSSPTPPVADNVTAFRRPAANRPT
                     PAITFGQTGKSPLPVALIFICLVVVYVGIRYFWR"
     gene            complement(5404..>5746)
                     /locus_tag="VM910_37320"
     CDS             complement(5404..>5746)
                     /locus_tag="VM910_37320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVI68202.1"
                     /translation="SATSATIVPGNRVQLVPYNQFAWWNKYQINPVWAAALGIIYFSD
                     SYASSDDTVRLPGFVRFDAGLFATIDEHWRAQLNVENIFNKGYWASADGNNNISPGQG
                     RTIRLKATYRF"
BASE COUNT         1156 a         1748 c         1779 g         1063 t
ORIGIN      
        1 aggccggctg aagtcttgga ttggttttcg gggaggggtc ttgggacgca atcttagagt
       61 gcttgtgagc ttgcttggtt tggcggcgcc gctgatggcg cagggcggcg cgacgccgcg
      121 ggattcgggc gcgacgctgc acatcgagag ccgagcggta ctggtagatg tgatcgtcac
      181 agaccagcat gggaatccgg tgacggggct gaagcaggat gcgttcagcg tgacggagca
      241 ggggaagccg cagccgacgg cgggctcgca atcctcaaga ctagcgacaa ggtgcgcatc
      301 gacctcaaca tgggaagcgc caacatcctg atttcggacg aggaggtcaa acgacgccac
      361 accgaattga aagccagggg cggtttcccc tacccggcca accagacgcc ttggcaggag
      421 ctctatcgct cgaccgtcgg tcagcaatcc acgggcgcct gcatggagtt tgccacgcgc
      481 tatcacgaca tcgcgggcaa ggtcggcgtg gcgagggata atcactgatg ccgtcattcc
      541 gggagaagcg ccagcccgga acccagaact accgccagtg accgtgaatt ccaggcctgc
      601 gcctcatggc gcatccggga gtgacgaaaa caataaggag caaccaaaaa tgtccgaccg
      661 tctcaagggc aagcgcgcct tcgtcaccgc cgcggccgcc ggcatcggcc gcgcctgcgc
      721 gctcgctttc gcgcgcgagg gtgcaagtgt gttcgcaacc gacatcgacg aaacgggtct
      781 cgccgcactc ataaacgagg gtatcaccga gatttcaaaa ctcgacgtgc gcgacagcgc
      841 cgcggtcaac gcgatcgcca ggcgcttcgg caaggtcgat atcctgctca acgcggcagg
      901 ctttgtgcat cacggcaccg tgctcgattg ctcggacgaa gactttgatt tttccttcga
      961 cctcaacgtc aagtcgatgc atcgcacgat ccgcgcattc ctgccggcaa tgctggaagg
     1021 aggcggtgca atcgtcaaca tctcctccgc cgccggcgtg ttcaaggcgg ctcccaaccg
     1081 ttacgtctac ggcgcgacca aagccgcggt cgctgcgctg acgcgctcgg ttgccgtcga
     1141 cttcatcagc aaaggcatcc gctgcaattg catctgtccc ggcaccatcg agacgccgtc
     1201 gatgctgcaa cgggcagcgg ccgcgggcgc cggcggacgc gagatgttcg tttcgcggca
     1261 gccgatggga cggctcggaa ccgcggagga gatcgcgtcg ctggcaatct acctggcgag
     1321 cgacgagagc gcattcacga ccggcgtcgc ccatgtcatc gacggcggct ggacgctatg
     1381 attcttcacc tctcccgccc gcggggaaag gccgcgcaaa gcgcggatga atggcaaggc
     1441 acaagaccca tctcacctga agtgtccttg gatcgaaaaa gcccctcacc ccaaccctct
     1501 ccccgcaaga gcggaaagag ggagcagact tcagcctagg cagaaataga gttcgacgaa
     1561 aggaccttcc catgaacaag atcgatctga acggccgcac ggccgtcgtc acgggcggcg
     1621 cgcagggatt cgggcgcgcg atcaccgagc gcttcgtggc gtccggcgcc aaggtcgcaa
     1681 tctgggacca cgatcaggcg ctggcggaaa agaccgcgaa ggaaatcggc aatgccgtct
     1741 cgccgttcaa ggtcgacgtg accgctatcg gcgcggtgga aaaaacccgc gacgcaaccg
     1801 tgtccgcgtt tggcaagatc gacatcctcg tcaacaatgc gggcattgcc ggggtcaaca
     1861 agacggtgtg ggagaccgat ttcgaagaat ggcgcaaggt gctgcgcatc aatctcgacg
     1921 gtcctttcat ctgctgcaag gcgatcgtgc cgcaaatgct caagcagaaa tacgggcgta
     1981 tcgtgaatat cgcttccatc gccggcaagg agggcaaccc caacgccgcg cattattcgg
     2041 catcaaaggc gggcctgatc gcgctgacca aatcgctcgg caaggaactc gccacgcacg
     2101 acattctcgt caacgcggtg acgcccgcgg ccgcgaaaac tgcaatcttc gaccagatga
     2161 cgaagcagca catcgacttc atgctgtcga aaattccgaa agcgcgcttc gtgctggtcg
     2221 aggaagtcgc agcgatggtc gcttggctcg cgtcggagga ctgcgctttt tccacgggcg
     2281 cggtgttcga catttcgggc ggacgggcga cttattaacg ggctgaatga ccgttcggcc
     2341 attcggggaa agacggcatc agctcgcacc ggttaccaac acgaacgaca gggagcagca
     2401 agtggccaag gcaccgccat cagccaagcg ggaccgcatc aggaatttca cgcctgcact
     2461 ggtcgattac acgaacgacg tcctctacgg cgacctctgg gagcgcaagg gtctttcgaa
     2521 gcgcgaccgc agcctcatca cggtcgcagc gctggttgca acctaccgtc cggagcaact
     2581 cgtctctcat ctcacgcgtg cgatcaacaa cggcgtaacg caggaggaga tcaccgaggt
     2641 cattacgcac atggcgtttt acgcgggatg gccggcagcg atgtcggcag cgcaggttgc
     2701 ctacacggtg cttgtcgaag gcaacaaggg ggactgacca atggcggagg acattgtcgt
     2761 cggctttatc ggactaggca cgatgggtgg caagatggcc accaacattc agaaggccgg
     2821 atacaaggtc atcgtgcatg atctgcatcg gcagtcggcg agccatcatt tgcaggccgg
     2881 cgccgaatgg gcgggcactc cacgggcgtt ggcggagaag tccgacgtca tcttcacctc
     2941 gctgcccgag ccggccgacg tcgagcgggt cgcacttggc gccgatggac tgatcgatgg
     3001 actgaagaag ggagctgcct atttcgatct gtcgacgaat tctccgggcg tcgtgaaaag
     3061 gatccatgac gccttcgccg cgaagggcgc gcgtatgctc gatgcgccgg taagcggcgg
     3121 gccgtcgggc gcagcgtcgc gcaaaatggc gatctgggtc ggcggcgaca aggccgcgtt
     3181 cgatcaatac aaggcagtgc tcgacgcgat gggcgaccgt ccggcctata ttggccagat
     3241 cggaagcgca acggtcgcga agctcgtcca caacatgtcg agctacgccc tcgcttgcgc
     3301 actagccgaa accttcacga tgggcgtgaa ggccggtatg gatcccgtcg cgctgtggga
     3361 ggccgtccgt cagggcgtca gtggccggcg cctcacattc gacggactgc tcgagcagtt
     3421 cttgcccggc aaatatgatc cgccgaactt cgcgctgaaa ctcgccacca aggacgtcgc
     3481 actcgcaact gcgctgggac gcgagcttgg agtgccgatg cgcatttgca atctcaccta
     3541 tgccgagatg atcgaagcgt gcaatcgcgg ctgggagggg cgcgactcgc gcgctgtgat
     3601 gctgctgcag caggagcggg cgggcgtgaa ggtcgccgcc gatccggagc gcgtgaagca
     3661 ggctgccgaa cgtgcaaaat ctggcgtcac ctgatgcgaa cttcggaatc ggaacgctcg
     3721 tccgggattt tgaacaaagc tccctttttt ggccgcggcc gacccgctgt cgtcgcggcg
     3781 aaaatgccga tgaaaaccgc cgcgatgccg accatgaggt tccaacgcag cggctcatcg
     3841 agaaggaccg cgccaaacag cgatgcggtg atcggattga ccgtaacgga tatcgccacg
     3901 cgcgtgggcg tcgttcgttc cagcgcgaac gcccacagat agaacgtaag tgcggcgccg
     3961 tcggtccgcc cgtgtctttg ccgcatattc gctgcttgat ggaatgtgcc ggcaaagaca
     4021 gccttaaagt gagcggctga agtcaagcta tccccgtaac ggaagccgtc gcctatttga
     4081 ttaccgaggt ctcctgcaag gcgtggtaac ttcgatccaa ccgtacagtc ccactggaac
     4141 ccagccgtga acatctcgct ctacgacatc tccgtctggg ttctgccgct ggtcatcgcc
     4201 atcaccttcc acgaggcggc gcatggcttt gtggcgcacc agctcggcga cgacacggcc
     4261 tataagcttg ggcgcgtcag cttcaacccg ctccggcata tcgatccgtt cgggacgctg
     4321 gtgctgccgg gcatgctgtt gttgtcccac tccccgtttc ttttcggata tgcgaagccc
     4381 gttcccgtga atttccgtgc gctccgcgag cctcgcgtcg gcatggtcct ggtggcactc
     4441 gcgggtcccg ccaccaacat cctgctggcg ctcgtagcgg ccgcctcgtt tcacatcctc
     4501 ggcttcgttc cggcgaacgc ggcgcagtgg ctggcggaga acctcaaaaa cgcggtcgtg
     4561 atcaatgtcg tgctcgcggt cttcaacatg atgccgatcc cgccgctcga cggtggccgg
     4621 gtcgcggtcg gtcttctgcc ccgcccgctc gcaatccccc ttgcccggct ggaaccgttc
     4681 ggcatgctga tcctgatcgg atttttgatc ctgcttccct tggcaggttc gcagttcggt
     4741 ctaaatcttg atgttatctc cgcaatactg cgaagatcga ccgatttgat tattcggttc
     4801 attcttgttc tgacaggtaa cgcctagtca caaggcgtga agagacccga ggccgtcttg
     4861 gtcaaagctg ccgatatgct gattgcgcga cgtgccgaca ccagggcccg cgccgacttt
     4921 gcgacctgga aaatgattgc caagctcaat ggcgcctctt ccctgcccgc cgaggcccag
     4981 aatttcctgg ccggatacag gacgctgctt gcaaagatga acgaggcgga cgccaccgaa
     5041 gcgaccatcc gcctgatgta caaaacctat tattcggaga tgggcggcgg agggtctccg
     5101 ccggagatcc gatcgagccc gacgccgccg gtggcagaca atgttaccgc tttccggcgg
     5161 ccggcggcca atcggccaac gcccgccatt acgttcgggc agacaggaaa atcgccgctt
     5221 ccggttgcgc tgatctttat ctgcctcgtg gtggtgtatg tcggcatccg gtatttctgg
     5281 cgctaaccga aagggtttgc ggtcgggttt caactgctct tgccctgcac cttgaaagtc
     5341 gccggcaagg agaactcagc ccctcatcga caatgagcgg cgacataact ccgttagaat
     5401 ctatcagaat ctataagtcg ccttcaggcg gatcgtccgt ccctgccccg gcgagatgtt
     5461 gttgttgccg tcggcagacg cccaatagcc cttgttgaag atgttttcga cattgagttg
     5521 ggcgcgccaa tgctcgtcga tcgtcgcgaa cagacccgcg tcgaaccgga caaaacctgg
     5581 cagccttacg gtgtcgtccg acgacgcgta ggaatcggag aaatagataa tgccgagcgc
     5641 cgccgcccag accggattga tctgatattt gttccaccag gcgaactggt tgtagggcac
     5701 gagttggacg cgattgccgg gaacgatggt cgccgaggtg gcgctg
//
LOCUS       DATLJT010001636         1583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1776607,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001636 DATLJT010000000
VERSION     DATLJT010001636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776607"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..705
                     /locus_tag="VM910_37325"
     CDS             73..705
                     /locus_tag="VM910_37325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68203.1"
                     /translation="MDAIATPKPKVLFEADPFIPYLREQDFDARLRPVVEPYKKRMGF
                     LPNALKLYAYRPEIAETLWKLNSNIMRDPSSALDQLLKRKLAAVACATNGCAYCTAHS
                     CSMLKKPKGLEFEGWGLSEEELQDIITGAYEPKNEMERACFDYVRSASEDPTSVPDEI
                     LQRLKQHLTPQQIVELACVVGFWKFYNTVHDSLHIPVESTLLRDTGYVDL"
     gene            692..1297
                     /locus_tag="VM910_37330"
     CDS             692..1297
                     /locus_tag="VM910_37330"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HVI68204.1"
                     /translation="MSISKLPRTRRRAKRANAAAAYVLPPTVSSGALLDKGSDRRFRA
                     LVNDLFTIASRMEIVRAHLGRRMGLSGPQYSVLIAVAHLQGERGVRVGTVAQAMHVSS
                     AFIASETGKMARLGLLLKRPNPQDRRGVLLTLAPAGRLKIDRVSAEIRAINDTFFGAL
                     DGESFAALCVTGQALVKGSGKAVQYVNAVESEPLSALSAAE"
BASE COUNT          304 a          492 c          493 g          294 t
ORIGIN      
        1 gatttcgaac gcgcctggcc ccgcaactgg ggcaaggtga agctatccga ctacctctga
       61 aggagagcca tcatggatgc catcgctacg cccaagccga aggttctgtt cgaagccgat
      121 cccttcattc cctatttgcg cgaacaggat ttcgacgccc ggctcaggcc cgtggtcgaa
      181 ccctacaaga aacgcatggg tttcctgccg aacgcgctca agctctacgc ctatcggccg
      241 gagatcgcgg agacgctgtg gaagctcaac agcaacatca tgcgcgaccc aagttcggcg
      301 ctcgaccaat tgttgaagcg caagctcgcg gcagtcgcct gcgccaccaa tggctgtgcc
      361 tattgcacgg cccatagctg ctcgatgttg aaaaagccga agggcctgga atttgagggc
      421 tgggggctga gcgaggaaga gctgcaagac atcattaccg gtgcctacga gccgaagaac
      481 gagatggaac gcgcctgctt cgactacgtt cgctcggctt ccgaggatcc gacctcggtt
      541 ccggatgaga ttttgcagcg gctcaagcag catctgacgc cgcagcaaat cgtcgagctc
      601 gcatgcgtgg tcgggttctg gaaattctac aacaccgtgc acgacagcct gcacatcccc
      661 gtggagtcga cgctcctgcg tgataccgga tatgtcgatc tctaagctcc cgcgcacccg
      721 caggagggca aaacgggcga atgccgcggc ggcctacgtc ttgcccccca ccgtctcctc
      781 cggcgcgttg ctcgataagg gctcggaccg tcggtttcgg gcgctcgtca atgatctctt
      841 cacgatcgca agccgtatgg agatcgttcg tgcgcatctg ggccgacgca tgggattgag
      901 cggaccgcaa tacagcgtgc tgatcgcagt cgctcatctc cagggcgaac gtggcgtcag
      961 ggtcggcacg gtcgcccagg cgatgcacgt gtcgagcgcc ttcatcgcct cggagaccgg
     1021 caagatggcg cggctcggat tgctgctcaa gcggcccaat ccgcaggacc gtcgcggcgt
     1081 gctgctcaca ctggcgcccg ccggccggct caagatcgac cgcgtcagcg ccgagatccg
     1141 tgcgatcaac gacacgttct tcggcgcgct cgatggcgaa tcctttgctg ccttgtgcgt
     1201 caccgggcag gcgctggtga aaggctccgg caaagccgtg caatacgtca acgcggtcga
     1261 gagcgaacca ttgtcggctc tgagcgcggc cgaatgagag tcgtgcgaga tatgccgccg
     1321 gaatgatcaa gtgcggcgcg aaaatgccct tgcggtcttt cgccgatgtg ccaacgttga
     1381 gcgcggctcg cggcgccgcg cgtggtgact ttcaccgctt cccgccattc agcggcgaag
     1441 tgacgcatga ggccatgatc ggcaaggtgg tgttctgatc gagggcaagg agcgcccgcc
     1501 gggcgcgaga tcgagggcat cgccattttc tcccagcaga tcgtaaacgg cctgatgctc
     1561 ggctccatct atgcgatggt cgc
//
LOCUS       DATLJT010001637         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2005428,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001637 DATLJT010000000
VERSION     DATLJT010001637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2005428"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VM910_37335"
     CDS             <1..816
                     /locus_tag="VM910_37335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68205.1"
                     /translation="SEQHTVMVDFGYTPEALNNNVDYVGADPKKIEALIVSHGHFDHY
                     GGLIGFLDKHRAVLPKDLTLYAGGEDNFCMRYQGPPGQLAEFGALDRREIASRNVKTV
                     LCETPVVIGHAFTTGKIKRTSTEKVLPNTLVEFAMKDGLGCNASHYLPAELLGKIVPD
                     EHIHEHATCFNVKDRGLVVISSCGHVGIVNSARQAMEVSGVNKIHALVGGFHLGPAAP
                     EYINQVMTEIKALDPDVIIPMHCSGDNFIQAVRAQTPDKLVLATTGARVTFGA"
     gene            824..1453
                     /locus_tag="VM910_37340"
     CDS             824..1453
                     /locus_tag="VM910_37340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVI68206.1"
                     /translation="MKGLLLAGAFAGGIAVAAAGWALWPQAPTERSATELMEALMWGR
                     EPVGGPFALIDHNGQPRTDADYRGKLLLIYFGFTFCSDVCPTELQAMASAIDQLGPAG
                     DAIQPLFITVDPEKDTPEQLKGYVTLFHPRLIGLTGDPRVIKKVALAYKAYYAKSDPT
                     RNAGTEVDHSSFIFLVGPDGKYLGFAPPGTSPGRLAEIISHALAHKAIE"
     gene            1603..2001
                     /locus_tag="VM910_37345"
     CDS             1603..2001
                     /locus_tag="VM910_37345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68207.1"
                     /translation="MGSLVTSKAIPKDADILVTINGTMDLGELARAGRRLKGSAQAIN
                     LGADIFLADVTGRYLGRICHYRECHPRVACRAQHCGRREHLNDDLHVITLSKQLIAAP
                     PLDLWPSVIRRLTVSADVEALLLTELERVQ"
     gene            2068..2205
                     /locus_tag="VM910_37350"
     CDS             2068..2205
                     /locus_tag="VM910_37350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68208.1"
                     /translation="MQFDHLRRREFVTLLAGGAAWPPAAQAQSCPATAACISRDALKV
                     Q"
BASE COUNT          447 a          734 c          708 g          455 t
ORIGIN      
        1 agcgagcagc acactgtgat ggtcgacttc ggctacaccc cagaagcgct caacaacaac
       61 gtcgattatg tcggtgccga cccgaagaag atcgaggcgt tgatcgtgag ccacggccat
      121 ttcgaccatt acggtgggct catcggcttt ctcgataagc accgcgcggt gctgccgaag
      181 gacctcacgc tctatgccgg cggcgaggac aatttctgca tgcgctacca gggaccgccc
      241 ggccagcttg ccgaatttgg cgcgctcgac cggcgcgaga ttgcgtcgcg caacgttaag
      301 accgtgctgt gcgagacacc cgtggtgatc gggcacgctt tcaccaccgg caagatcaag
      361 cgcacctcga ccgaaaaagt gctgccaaac acgctggtcg agttcgccat gaaggacggt
      421 ctcggctgca atgctagcca ctatctgccc gccgaactgc tgggcaagat cgtgccggac
      481 gaacatatcc acgagcacgc gacctgcttc aatgtgaagg atcgcggtct ggtggtgatc
      541 agctcctgcg gccacgttgg catcgtcaac tcggcgcgcc aagccatgga agtgtcgggc
      601 gttaacaaaa tccacgcgct ggtcggaggc ttccacctcg gcccggccgc gcccgagtat
      661 atcaatcagg tcatgaccga gatcaaggcg ctcgatccgg acgtcatcat cccgatgcac
      721 tgcagcggcg acaacttcat ccaggcggtg cgcgcgcaga cgccagacaa gctggtgctg
      781 gcgacaaccg gcgcccgcgt cacgtttggc gcctgagggc gcggtgaagg ggcttctgct
      841 ggctggtgcc tttgccggcg gcatcgctgt cgccgccgcc ggctgggcgc tatggccgca
      901 agcgccgacg gaacgttcgg cgacggagtt gatggaagca ctgatgtggg gccgcgagcc
      961 ggtcggcggg ccgttcgcgc tgatcgacca caacggccag ccccgcactg acgcggacta
     1021 ccgcggcaag ctgctgttga tctactttgg ctttacgttc tgctccgacg tgtgcccaac
     1081 cgaattgcag gcgatggcgt cggcgatcga ccagcttggt ccggccggcg acgcgatcca
     1141 gccattgttc atcacagtcg atccggaaaa ggacacgcct gagcagctca agggttacgt
     1201 cacgctattt catccccggc tcatcggcct gactggcgac ccacgcgtca tcaagaaagt
     1261 cgcgctggcc tacaaggcct attacgccaa atccgatccg acgcggaatg ccgggaccga
     1321 agtcgaccac agcagcttca ttttcctggt cggcccggac ggcaagtacc tgggcttcgc
     1381 gccgcctgga acgtcgccgg gacgcttggc cgaaatcatt tcgcatgctc tggcgcacaa
     1441 agcgatcgaa taaggcgtga accagcatca agcgagatgc gtcgcagtcc atcccacggc
     1501 cctaggccct cggtttccga accgcggcgg gagcttctcc tcgctgtacg gtccttcgtg
     1561 tgggcggcat gtacttgtcc gggcgttctg cgtattgccc tcatgggctc gctggtgaca
     1621 agcaaagcga ttccgaagga cgccgatatc ctcgtcacga tcaacggtac gatggatttg
     1681 ggcgaacttg cgcgcgctgg tcgacgcctt aagggatctg ctcaagccat caacctcggc
     1741 gccgacatct tcctcgccga tgtgactggt cgttatcttg gtcgcatctg ccattatcgc
     1801 gagtgccatc ctcgtgtggc atgtcgcgct cagcattgcg ggcgccgcga gcacctcaat
     1861 gacgaccttc atgtcataac gctatcgaag cagctgattg ctgcgccgcc gcttgacctt
     1921 tggccaagtg ttatccgccg actcacggtg tcggctgatg tagaggcatt attgctgact
     1981 gagctcgaac gagttcaata gtacggcgca ttcgaatgcc ctgaacggat cgttcgcgac
     2041 gatcacggtt ttgctgacgg gaaggagatg cagttcgatc acttgagacg ccgcgagttc
     2101 gtcacgctgc tcgccggcgg ggcggcgtgg ccgccggctg cgcaagcgca gtcctgccct
     2161 gcgacggcag cctgcatctc ccgggacgcg ttgaaggtcc aataaatgac aactccctct
     2221 ttcacccgcc ggacagcaat cgtcgatgcg cacgctcatc tctacgacgg cggagccaac
     2281 cgctacggta tttttgcaag gaaagatccg ggatttgaag cttttgtcgg ggattactcc
     2341 gcgc
//
LOCUS       DATLJT010001638         1606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_165888,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001638 DATLJT010000000
VERSION     DATLJT010001638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165888"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VM910_37355"
     CDS             complement(<1..809)
                     /locus_tag="VM910_37355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVI68209.1"
                     /translation="MDKTVNQKAWFLVLPVFAVVAFSAVLPLMTVVNYSVQDTFGNNQ
                     FFWNGVGWFKELLDPSSDLGSRFFASLWRNLFFSAVILAIEVPLGIVIALSMPREGWQ
                     VAACLVILALPLLIPWNVVGTIWQIFGRGDIGLLGYVLNHIGINYNYVANEIDAWVTI
                     IVMDVWHWTSLVALLCYAGLKSIPDAYYQAAQIDGASRWAMFTAIQLPKMNRVLLIAV
                     LLRFMDSFMIYTEPFVVTGGGPGNSTTFISIELVKIALGQFDLGKAAALSLV"
     gene            complement(809..>1606)
                     /locus_tag="VM910_37360"
     CDS             complement(809..>1606)
                     /locus_tag="VM910_37360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68210.1"
                     /translation="MTVGQNLAFPLKNRGVSKGEIDKRVADIAKLLDLTPDLNRKATR
                     LTADAKQKISLGRGLVRSDVAAVLFDEPLTVIDPELKWQLRSKLKALHRELDLTMIYV
                     THDQTEALTFADTVVVIHDGRVVQSGTPAELFGKPAHTFVGYFIGSPGMNIVPAEVSG
                     REARIGGHVIALNRTYSGLNGAKIEIGVRPEFVDVATPAPDLLTATIERIDDLGRMRF
                     ARVRVGDAKFAARVPQGFSPSGDTAGLRFDPAHVHVYADSLLIEGTA"
BASE COUNT          336 a          499 c          488 g          283 t
ORIGIN      
        1 accaaggaca gcgccgcggc cttgccgagg tcgaactggc cgagcgcgat cttgacgagc
       61 tcgatcgaga tgaaggtcgt ggaattgccg gggccaccgc cggtgacgac gaacggctct
      121 gtgtagatca tgaaactatc cataaagcgc agcagcaccg cgatcagtag cacgcggttc
      181 atcttcggca gctggatcgc ggtgaacatc gcccagcgcg atgcgccgtc gatttgcgcg
      241 gcctggtaat aggcgtcggg gatcgacttc aacccggcgt agcagagcaa cgcgacgagg
      301 ctcgtccaat gccagacgtc catcacaatg atagtgaccc aggcgtcgat ttcgttggcg
      361 acataattgt agttgatgcc gatatggttc agcacatagc cgagcagccc gatgtcgccg
      421 cgcccaaaga tctgccagat ggtaccgacc acgttccacg ggatcaaaag cggcagcgcc
      481 aggatcacca gacaggccgc gacttgccag ccctcgcgcg gcatcgacaa agcgatcacg
      541 atgccgagcg gcacctcgat tgcgaggatc accgcggaga agaacagatt gcgccagagc
      601 gaggcaaaga agcggctgcc gagatcggac gagggatcga gcaattcctt gaaccagcca
      661 acgccgttcc agaagaactg gttgttgccg aacgtgtcct gtaccgaata gttcaccacc
      721 gtcatcagcg gaaggaccgc tgagaacgcg accacggcga acaccggcag cacaaggaac
      781 cacgccttct ggttgacggt cttgtccatc acgccgtccc ctcgatcaat aggctgtcgg
      841 cgtagacatg cacatgcgcg ggatcgaatc tgaggcctgc ggtgtcgccc gacggcgaga
      901 agccctgcgg cacgcgcgcg gcgaatttcg cgtcgcccac ccgcacgcgc gcaaagcgca
      961 tcctgccgag atcgtcgatc cgctcgattg tcgctgttag caggtccggc gcgggcgtgg
     1021 cgacatcaac gaattcgggc cgcacgccga tctcgatttt cgcgccgttc aacccgctgt
     1081 aagttcggtt cagcgcaatg acgtggccgc cgatccgagc ctcgcgcccg ctcacttcgg
     1141 ccggcacgat gttcatgccg ggcgagccga tgaaatagcc gacaaaggta tgcgcgggtt
     1201 ttccaaagag ttcggccggc gtgccgctct gcaccacacg cccgtcatgt atcacgacga
     1261 cggtatcggc gaaggtcagc gcttcggtct gatcgtgggt gacatagatc atcgttagat
     1321 cgagctcacg atgcagcgcc ttcaattttg agcgcagctg ccatttcaat tcgggatcga
     1381 tcaccgtcag cggctcgtcg aacagcacgg ccgcaacgtc ggagcgcacg aggccgcgtc
     1441 ccagcgagat cttctgcttg gcatccgcgg tgaggcgcgt cgccttgcga ttgaggtcgg
     1501 gcgtcaagtc caacagcttt gcgatgtcag cgacgcgctt gtcgatctcg cccttcgaca
     1561 caccgcggtt cttcagcggg aacgcgagat tctgaccgac cgtcat
//
LOCUS       DATLJT010001639         1958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2464799,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001639 DATLJT010000000
VERSION     DATLJT010001639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464799"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VM910_37365"
     CDS             <1..270
                     /locus_tag="VM910_37365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HVI68211.1"
                     /translation="VFFAWGEIYSLFPATCTDIYGRKFATTNYGMLYTAKGTASLLVP
                     LANVLTSATGSWHAVFYVAAILNIIAAVMALAVLRPMRRRAMEMG"
     gene            complement(369..527)
                     /locus_tag="VM910_37370"
     CDS             complement(369..527)
                     /locus_tag="VM910_37370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68212.1"
                     /translation="MSDLAARFVYSPLLSTPFKVVCLFCVLGLVLSAAIVPMIAPEYM
                     AWVLSHIE"
     gene            770..1525
                     /locus_tag="VM910_37375"
     CDS             770..1525
                     /locus_tag="VM910_37375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVI68213.1"
                     /translation="MSQGPRNPRPVSVPGPSAAHPAGAAISPRVEPIASPDSFKTRAY
                     AALKDAIVAMDVYRSRSDIRLDERRLALDFGISRTPVREAMAQLEREGFVRAVPRRGI
                     YVVRKTKREVIEMITAWAALESMAARLITENASADEIASLRTMFAKFENGELHARLDE
                     YSEVNIEFHQTIIRMSRNGVLISLAENLFTHMRMIRRRTIGEQDRADRSIRDHLSIIE
                     ALEARDTKRAEDLVRNHALGLAEHVEKYADYLD"
     gene            1557..>1958
                     /locus_tag="VM910_37380"
     CDS             1557..>1958
                     /locus_tag="VM910_37380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI68214.1"
                     /translation="MAEAAVKPKPADTAAQDAERELTDGFHLVIDALKLNGINTIYGV
                     PGIPITDLGRMAQAAGMRVISFRHEQNAGNAAAIAGFLTKRPGVCLTVSAPGFLNGLT
                     ALANATTNCFPMILISGSSEREIVDLQQGDYE"
BASE COUNT          375 a          637 c          619 g          327 t
ORIGIN      
        1 gtgttcttcg cctggggcga gatctactcg ctcttccccg cgacctgcac cgacatctac
       61 ggcaggaagt tcgcgacgac gaattacggc atgctttata cggcgaaggg gacggcatcg
      121 ctgctggtcc ccttggccaa cgtgttgacc agcgcaaccg gcagctggca cgccgtattt
      181 tatgtcgcgg cgatcctcaa catcattgcg gcggtcatgg cgcttgcggt gttgcggccg
      241 atgcgacgcc gcgcaatgga aatgggttga cggtgccggg aaacgcggac cgtccgcgcc
      301 gttgtcgcgc ggacggtccg tcgaactctg tggtcggcgc agcgaatgac gtcatggcaa
      361 cggaaacgtc attcgatgtg agacagcacc caagccatat actccggcgc gatcatcgga
      421 acgattgctg ccgagagcac caaacccaac acacagaaca ggcacacgac cttgaacggg
      481 gtcgaaagga gcggcgaata gacaaagcga gcggcaagat cggacatcgg ttcctcctcg
      541 gcatcgcacc gatgtcgtag cgctgcgcgc gcgtgcgccg tgtgaaccgg ttcacctgcc
      601 cggcgctttt ttcgctgctg ccgctcggtc gttgatcgca ggttggcgac cgctgcggga
      661 ggtcagcggt gcggatgcgg cgcgcgaccc ccactttgtg cgaatgccgg atggggagga
      721 aaacgcgtcg gttgcatcgg ttcaagccct ggaatattgt ataccagcca tgagtcaggg
      781 tcctcgtaac ccccgtcccg tcagcgtccc gggcccatcc gcggcgcacc ccgcgggtgc
      841 cgcgatatcg ccccgcgtcg agccgatcgc cagcccggac agcttcaaga cccgcgccta
      901 cgcggcgctc aaagacgcca tcgtggcgat ggatgtgtat cgcagccgct ccgacatccg
      961 cctcgacgag cggcggctcg ccttggattt cggcatcagc cgcacgcccg tgcgcgaagc
     1021 gatggcccag ctcgagcgcg aaggtttcgt gcgcgcggtt ccccggcgcg ggatttacgt
     1081 cgttcgcaag accaagcgcg aggtgatcga aatgatcacc gcttgggccg cgctcgagag
     1141 catggccgcg cggctcatca ccgagaacgc cagcgccgac gagatcgcgt cgctgcgcac
     1201 catgttcgcg aagttcgaga acggcgagtt gcacgccagg ctcgacgaat attccgaggt
     1261 caacatcgag ttccatcaga ccatcatccg catgagccgc aacggtgtgt tgatctcgct
     1321 cgcggagaac ctgttcaccc atatgcggat gattcggcgc aggaccatcg gcgagcagga
     1381 ccgcgccgac cgctcgatcc gcgaccatct cagcatcatc gaggcgctcg aggcgcgcga
     1441 caccaagcgc gccgaggacc tcgtgcgcaa ccacgcgctc ggcttggccg agcacgtcga
     1501 gaaatacgcc gactatttgg actgacgcgg aaaaaagttt cgagggagga ttcgccatgg
     1561 ctgaggcagc agtgaaaccc aagcccgcgg acacagccgc gcaagacgcg gagcgggagc
     1621 tgacggacgg cttccacctc gtcatcgacg cgctcaagct caacgggatc aacacgatct
     1681 acggcgtgcc cggcatcccg attacggatt tgggccgcat ggcccaggcc gcgggcatgc
     1741 gcgtcatctc gttccggcac gagcagaacg ccggcaatgc ggctgcgatc gccggcttcc
     1801 tgacgaaaag gcccggtgtc tgcctcacgg tgtcggctcc cggctttctc aacggtctga
     1861 cggcgctggc caatgccacg accaactgct tcccgatgat cctgatcagc ggctcgtccg
     1921 agcgcgagat cgtcgacctg cagcagggcg actacgaa
//
LOCUS       DATLJT010001640         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2924240,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001640 DATLJT010000000
VERSION     DATLJT010001640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2924240"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1161)
                     /locus_tag="VM910_37385"
     CDS             complement(55..1161)
                     /locus_tag="VM910_37385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68215.1"
                     /translation="MISPTRLTRRGFLYASGAAAAIAGTNLARPAIAQPAKVRYTLSW
                     LPTGQYAFLYCARQLGYFKQCGIELDIARGYGSMAAIQAVSTGQFEMGGAQTGANLLS
                     IMKGLDLRLLGTQGYDATLGVLALEKGPVKTPKDLEGRKIGVSAAGGDTPFLPAYCRL
                     VGVDFDKVTVVQLDSKILEQSAMSGVVDAIVVTGLSSIPNFVSEDVPFRMMPFSQAGL
                     QFYWVNTITTGSFLERNKELVDNVQMGILEGMKFMLLNPQEAMERHLKEHEELALGKN
                     GRLYVELGLGMTRAIMAAPESMEHGLGYTDLAKIDEQAKVVKQYMAAPKDPEPPKAET
                     FCRNNAVGKVILTPAQWGEVRSSTQKYMKLLGKA"
     gene            1301..2215
                     /locus_tag="VM910_37390"
     CDS             1301..2215
                     /locus_tag="VM910_37390"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68216.1"
                     /translation="MRRLLTYGVPTDRCGLQLRLGVEKGFFCEEGIDLELRVVFGGPE
                     IAAELDAGRLLVGELGAPPGLTAMANGARFKIVGSGVRRGAVLYFVARKQFSTFADLK
                     RARLGVLSKGSCSDWYMRELLCHRGLDPEAHVTIVGLGQRYPEVLTLLSDGDLDGALI
                     AEPHVSMGEAAGHFNVWWGVNACDFAPRMQWTIMVANDDLLLHEPDLIRRVLSACRRS
                     YRYAAKHREEWADFGARYFRIPRSTMMRSIERELPDLHFDCEVDMEGLAAAVALQQRL
                     GGVPASLRLNDIVDPRFTVIAAQPAAAG"
     gene            2242..>3157
                     /locus_tag="VM910_37395"
     CDS             2242..>3157
                     /locus_tag="VM910_37395"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI68217.1"
                     /translation="MSGHTPARTETHDRWHATADVVVVGYGYAGAVAAIEAHDAGADV
                     LLIEKMPDPGGISITSGGNVRFVEDVEEGFRHLQATSAGTTPDPVLRALAAGMAEIPA
                     YFGKLARVCRATINRRQADGNYPFPGTKTFGYVSIDHVPGFDAERTYPFVSSYVPIHR
                     AAGVRLFKVLQDNISSRKVRVELSAPATRLITGAHGEICGVMAQRDGRMFAIKARRAV
                     VLACGGFEANSDMQSQYWQEKPVLNAAYMGNTGDGILMAQAVGAALWHMWHYHGVYGF
                     KHPDPAYPFGIRPKRLPDWIPGENARADV"
BASE COUNT          567 a          949 c          961 g          680 t
ORIGIN      
        1 aagactttgc gtacccgatg gcagctgatt tccgcctgca tgcgtgctgc gcgcttacgc
       61 cttaccaaga agtttcatgt atttctgcgt gctcgagcgc acttcacccc attgcgcggg
      121 ggtcaatatc actttcccga ccgcattgtt gcggcagaat gtttccgctt tcgggggctc
      181 agggtccttc ggcgccgcca tgtactgctt caccaccttc gcttgctcgt cgatcttggc
      241 caagtcggta tagcccagcc catgctccat cgattcggga gctgccatga tggcgcgcgt
      301 catgcccagc ccaagctcga cgtagagtcg cccattctta cccagtgcga gctcctcatg
      361 ctccttgaga tggcgctcca tcgcctcctg cggattgagc agcatgaatt tcatcccttc
      421 caggatgccc atctgcacgt tgtccacaag ctccttgttc ctttcgagaa agctgccggt
      481 cgtgatcgtg ttgacccaat agaactgaag ccctgcttgc gagaagggca tcattctgaa
      541 tgggacatct tccgagacga aattcgggat cgagcttagt ccggtcacca caatcgcatc
      601 gacaacgccg gacatcgcgg actgctcgag gatcttgctg tcgagctgga caacggtcac
      661 cttgtcgaag tccacgccca cgaggcggca ataggccggc aggaatggcg tgtcgccgcc
      721 tgccgccgaa acgccgatct tcctgccctc gagatctttc ggtgtcttta cgggtccctt
      781 ttcaagcgcg aggaccccca acgtcgcgtc gtaaccctgg gtgccaagaa gccgtagatc
      841 gagacccttc atgatcgaga gcaggtttgc gccggtctgc gcgccgccca tctcgaattg
      901 accggttgac accgcctgga tggccgccat cgagccgtaa ccgcgcgcga tgtcgagctc
      961 gataccgcac tgcttgaaat atccaagctg ccgggcgcaa taaagaaacg catactgtcc
     1021 ggtcggcagc caggaaagcg tgtagcgaac cttcgccggc tgggcgatcg cgggcctcgc
     1081 taggtttgtt cctgcgatcg cggcagccgc gccgctcgcg tacaagaatc cgcgccgcgt
     1141 cagtcgtgtc ggtgaaatca tggttgttcc ctccgcttcc agctatattc gtggaagccg
     1201 cgggagactt gcaagtgcgt tatatgcgcg cggcggcggg tttcctgccg gctttttagg
     1261 cgtgggtcca gagcggttgt tttttgcaga ggctgcattc atgcgaagac tcctcaccta
     1321 cggcgtgccg acggatcgat gcggcctgca gctgcgactg ggagtcgaga agggtttctt
     1381 ttgcgaggag gggattgacc tggagcttcg cgttgttttc ggcggcccgg aaattgctgc
     1441 cgagcttgat gcggggaggc ttcttgtcgg tgagctgggc gcacccccag ggctgacggc
     1501 catggcaaac ggggcccgct tcaagatcgt aggcagcggt gtgcggcgtg gcgcagttct
     1561 ttattttgtc gcacgcaagc agttttccac ctttgcagat ctcaagcggg cgcggctcgg
     1621 cgtcctgagc aaaggcagct gcagcgattg gtacatgcgc gagctgttgt gccatcgggg
     1681 ccttgatcct gaggcccatg taaccatcgt cggtctggga cagcgctatc ccgaggttct
     1741 caccctacta tctgacggag atctcgacgg tgccctcatt gcggaaccac acgtcagcat
     1801 gggagaggcg gctggccatt tcaatgtctg gtggggggtc aacgcctgcg atttcgcccc
     1861 acggatgcaa tggacgatca tggttgcaaa cgacgatttg cttctgcacg agcccgacct
     1921 tatccgccgc gttctgtccg cgtgtcgtcg cagctatcgc tatgccgcga agcaccgcga
     1981 ggaatgggcc gactttggcg cccgatactt tcgtatccca cgcagcacca tgatgaggtc
     2041 tatcgagcgc gagcttcctg atctgcattt cgactgtgag gttgatatgg agggactcgc
     2101 agcggccgtc gcgcttcagc aaaggctcgg cggcgttccc gcttcccttc gattaaacga
     2161 tatagtggat ccgcgcttca ccgtgattgc cgcgcagccg gcggctgcgg gttgagcaag
     2221 cgcttggggt ctgacgcgcg aatgagcggg catacgccgg cacgtaccga aacgcacgac
     2281 cgttggcacg cgacggccga tgtggtcgta gtgggctatg gctatgccgg ggccgtcgcc
     2341 gccatcgaag cgcatgatgc tggcgccgac gtgctcctta tcgaaaagat gccggacccc
     2401 ggcggcatct ccatcacctc gggcggcaat gttcgctttg tcgaggacgt ggaagaaggc
     2461 ttccgtcatc tgcaggcgac cagcgcgggc acgacacctg atccagtgtt gcgagccctt
     2521 gccgcaggga tggcggaaat cccggcctac ttcgggaagc tggcacgcgt ctgcagggca
     2581 accatcaatc ggcgacaagc tgacggcaat tatccttttc ccggcacgaa aaccttcggt
     2641 tacgtcagca tcgatcatgt gccgggattt gacgccgagc ggacctatcc attcgtttcg
     2701 agctatgtgc cgatccatcg cgcggcaggt gtgcgccttt tcaaggtgct ccaggacaat
     2761 atttcctcgc gcaaggttcg ggttgagctt tcagcccctg caactcgctt gatcaccggc
     2821 gcgcatggcg aaatttgtgg ggtgatggcg caacgcgatg ggcgaatgtt cgctatcaag
     2881 gcccgccgcg ctgtcgttct cgcttgcggc ggcttcgagg caaactccga catgcagagc
     2941 cagtactggc aggaaaagcc tgtgctcaat gccgcgtata tgggcaacac cggagacgga
     3001 atcctgatgg cgcaggcggt cggggcggcg ctctggcaca tgtggcacta tcacggcgtc
     3061 tacggtttca agcaccccga tcctgcttat cccttcggca tccggccgaa acggctgccg
     3121 gactggatac caggggagaa tgcgcgcgcc gacgtca
//
LOCUS       DATLJT010001641         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1778120,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001641 DATLJT010000000
VERSION     DATLJT010001641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778120"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VM910_37400"
     CDS             <1..702
                     /locus_tag="VM910_37400"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HVI68218.1"
                     /translation="STIVPFTGAGISTECGIPDFRSPGGLWTRNRPISFDEFVASQKA
                     RDESWRRRFAMEETFKAAKPGRGHRALASLYRAGKVPAIITQNIDNLHQASGFAPDDV
                     IELHGNTTYARCIGCGRAYELAWVKQRLEENGMAPDCADCFEPVKTATISFGQAMPEE
                     AMQRASELAQHCDLFLAIGSSLVVWPAAGFPMMAKNCGAKLVIINREPTDQDDIADLV
                     IRHDIGETLGPFVGN"
     gene            927..1586
                     /locus_tag="VM910_37405"
     CDS             927..1586
                     /locus_tag="VM910_37405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVI68219.1"
                     /translation="MGSDGFESKKVGVPPGGTGPNRLGTNDYASGAARDSAMEAVSGI
                     GDTASLVEIPGVIKWFDASKGYGFIVPDNGWPDVLLHVTVLRRDGYQTAYEGARVVVE
                     CVQRAKGYQAFRILSMDESTAIHPAQMLPPRTHVTVTATSGLERAQVKWFNRLRGFGF
                     LTCGEGTPDIFVHMETLRRFGMTELRPGQYVLVRFGPGSKGMMAAEIHPETGSPGLSS
                     H"
     gene            1735..1811
                     /locus_tag="VM910_37410"
     tRNA            1735..1811
                     /locus_tag="VM910_37410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1769..1771,aa:Pro,seq:tgg)
     gene            complement(2008..2322)
                     /locus_tag="VM910_37415"
     CDS             complement(2008..2322)
                     /locus_tag="VM910_37415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVI68220.1"
                     /translation="MGSLAVAALLIDWPLNVAPASAANVDQGRRLASHYCARCHSIDK
                     TQPSPLAGAPPFRTLHERYPVDTLQEALAEGIVTGHPDMPEFRFDPTQIGDFLAFLKT
                     LE"
     gene            complement(2460..4283)
                     /locus_tag="VM910_37420"
     CDS             complement(2460..4283)
                     /locus_tag="VM910_37420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HVI68221.1"
                     /translation="MAIGALGVVYGDIGTSPLYTFKTAIEWAGGEATPAVALGILSLV
                     VWTLIITTSLKYVAIVMRADNDGEGGILALMSLIGIKHGERPFVIALGIIGAALLYGD
                     GAITPAISVLSALEGLKDPLPSFAPYVLPLSVLVLIAVFALQPQGTARIGALFGPIMT
                     IWFISIGLLGLGGIIDHPSVLAALDPRYGLSYLFGHGFTGFLVLGGVFLCATGAEALY
                     ADMGHFGARPIRLTWYGLVLPTLVLNYAGQTAILVEGKTASGSNPFFLLCPERLQLPL
                     VALATVATIIASQAIISGTFSMTRQAIQLGLCPRLYIKQTSSQGYGQIYVGFVNWTLM
                     TLTLALAVSFRSSDNLASAFGIAVALTMFLTSVLMFQAMREVWGWSFWLAAAVAGFFM
                     IVDLSFVAANMMKVMEGGWVPLVVGATIYFLMSTWHRGRLALREQLDRDTLPLVDFIG
                     SMRNKARVPGTAVYLTSRLDMVPVPLLHNLKHNKVLHQRIVLLNIDTAHIPRVPPERR
                     LTINDLGDNFYTVLAEYGFMQQPNLPRVLFRCGGNGLSFNMMDTSFFVGRVKIVPAGK
                     SRLGHFRCSLFEFMHRNAMAATDFFRIPAGRVIELGSQAEI"
     gene            4379..4765
                     /locus_tag="VM910_37425"
     CDS             4379..4765
                     /locus_tag="VM910_37425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68222.1"
                     /translation="MARQVPRRGPGISRSLRRRLNLGNARHAEMTAGSLMGCGFVKKE
                     AGLLADIHQQDDGEEQSDAKERRIGALRALRTLVQTAYPSARPVPDTNRGQLRNGKSH
                     DCLEACFGHNVLGRPFKIQIGRQLAL"
     gene            5175..>5956
                     /locus_tag="VM910_37430"
     CDS             5175..>5956
                     /locus_tag="VM910_37430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVI68223.1"
                     /translation="MNVFGYLLKHSRLAVIVVAAIGAAALLWSRGFVGRADGQATRKE
                     LVASARSEQTGSIGSGEESVDLSQKQAGSIKVGTLGARKFAILKTAVGTIDFNENMLV
                     QVFTQYQGKIIKAFYNVGDDVKQGDILFTIDSPDLLQAESTLLAAAGVLELQKKTLAR
                     VSGLLKTGGAAQKDVDQSTSDQQTAEGNFKAAKDAVRIFGRLDEEIDEILVNRRVNST
                     LKVPSPISGRIVTRNAAPGLLTQPGTPPPPYQMADVSTMWMVA"
BASE COUNT         1246 a         1703 c         1903 g         1104 t
ORIGIN      
        1 tccacgatcg tgcctttcac cggtgccggc atctccactg agtgcgggat tcccgatttt
       61 cgttcgccgg gcgggttgtg gacccgcaat cggccgattt cctttgacga gttcgtcgca
      121 agccagaagg cgcgcgacga atcctggcga cgtcgcttcg cgatggaaga gaccttcaag
      181 gccgccaaac ccgggcgcgg tcatcgcgcg ctcgcctcgc tctatcgtgc aggcaaggta
      241 ccggcgatca tcacccagaa catcgacaat ctgcatcagg cgtcgggatt cgcgcccgac
      301 gacgtcatcg agctgcacgg caacacgacc tatgcgcgct gcatcggctg cggacgcgct
      361 tacgaactcg cctgggtcaa gcaacgcctt gaggagaacg ggatggctcc cgattgcgcc
      421 gactgttttg agccggtcaa gaccgcaacc atctcgttcg gacaggcgat gccggaagag
      481 gcaatgcaac gcgcaagcga actcgcacag cattgcgacc tgtttctcgc gatcggatcg
      541 tcactggtcg tgtggcccgc tgccggtttc ccgatgatgg ccaagaattg cggtgccaag
      601 ctcgtgatca tcaaccgcga gcccaccgat caggacgaca tcgccgacct cgtgatccgc
      661 catgacatcg gggaaacact cgggccattc gtcggtaact aaagatcgcc ggcggatccg
      721 cacatcggcc gcgatcgatt cgcgcctgtg caaggttgtt cataggttcc ggcaattttg
      781 ttttttatct atgcgccgca atcgggagtg ttatcttttg attcgtggag attcgcgcgt
      841 catcaacaac aattgccgat gagcgtgcgc gtttcgggac cgtcgcaaac gcgacgaccg
      901 ttaaatgatg tgtggggtcc ggggttatgg ggtcggacgg atttgagtcc aagaaggtcg
      961 gggttccgcc gggggggacc ggaccgaacc gtcttgggac taatgattat gccagcggtg
     1021 ccgcgcgcga ctccgcgatg gaagcggtgt ccgggatcgg tgataccgcc agcctcgtcg
     1081 agattcccgg cgtgatcaaa tggttcgatg cttcgaaggg ctatggcttc atcgttcccg
     1141 acaatggctg gccggatgtg cttttgcacg ttaccgtgct taggcgcgac ggctaccaga
     1201 cggcttacga gggcgcccgc gtcgtggtgg agtgcgtaca gcgcgccaag ggctaccaag
     1261 ccttccgtat tctctcgatg gacgaatcga ctgcgattca cccggcgcaa atgttaccgc
     1321 cgcgaacgca tgtcacggtt accgcgacca gcgggcttga gcgcgcgcag gtcaaatggt
     1381 tcaaccggct gcgcggattc ggcttcctga cctgcgggga ggggacgccg gacatctttg
     1441 tccacatgga gacgctgcgc cggttcggga tgaccgaact caggccaggt cagtatgtgc
     1501 tcgtgcggtt cgggccgggt tccaaaggca tgatggcggc cgaaattcac ccggaaaccg
     1561 gttcgccagg tctgtcttcg cactagcgcg ccgccgatcc gcaaaaaatt tgggcacgga
     1621 cgtgatgatt cggcccaaat ccgcttacca taaggctttg aggcccgtcc ctttggtggc
     1681 gctgcttgct gctggcggct tgagcgtgta tcgacgacgt tattctgggg atatcggggt
     1741 atagcgcagc ctggtagcgc ggcagttttg ggtactgcag gtccgcggtt cgaatccgcg
     1801 tgccccgacc aagaaaatca agtccagcaa taaaaaccaa ggcccgatca ggccgcactc
     1861 gcttcgcgag atcgggccgg gcgctatggc accagccgaa ttccggtgat gaagttcgcg
     1921 aatcggcccg cgcgtcaact actgctttgc cggctacaag tattgatctc ccggatcctg
     1981 caacatcgct ttggagagcg cgcggcccta ttccagcgtc ttcagaaacg cgagaaagtc
     2041 tccaatctgg gttggatcga agcgaaattc cggcatgtcc ggatgaccag tgacgatacc
     2101 ttccgccaag gcttcctgca gggtatcgac aggatagcgc tcgtgcagcg tgcgaaacgg
     2161 cggcgcgccc gcaagcgggc tcggctgcgt cttgtcgatc gaatggcagc gtgcgcaata
     2221 gtggctcgcc agacggcggc cctggtcaac atttgctgcg gaggctgggg cgacgttcag
     2281 cggccagtcg atcagcaggg ccgcgaccgc caacgaaccc atgagggtgg tgtgagcgcc
     2341 ggagcttcgt cgggaaatgg acatagcggc aacatatcgg cgccgcatgc ggcattgatt
     2401 gacgcaagtc aacgcggaaa ggtgcctgca agaggctcag acaagctcag cgcacgtatt
     2461 caaatctccg cctggctgcc aagctcgatt acgcggcccg ctggaatgcg gaagaagtcg
     2521 gtcgcggcca tcgcgttgcg atgcatgaac tcaaacaggc tgcaacggaa atggccgagc
     2581 cttgatttgc ctgccggcac gattttgacg cgtccgacga agaacgaggt gtccatcatg
     2641 ttgaatgaca ggccgtttcc accgcagcgg aacagcacgc gcggcaggtt cggttgctgc
     2701 atgaagccgt attcagccag gacggtgtaa aaattgtcgc cgaggtcgtt gatcgtgagg
     2761 cggcgctcgg gtggtacgcg aggaatgtga gcggtgtcga tgttcaacag cacgatgcgc
     2821 tggtgcagca ccttgttgtg cttcagattg tgcaacagcg gcaccggcac catgtcgagc
     2881 cggctggtca ggtacacggc ggtgccaggc acgcgcgcct tgttgcgcat agagccgatg
     2941 aaatcgacga gcggcagggt atcgcggtca agctgctcgc gcagcgccag gcggccacga
     3001 tgccaggtcg acatcaggaa gtagatcgtc gcgccgacca cgagcggcac ccagccgcct
     3061 tccatgacct tcatcatgtt ggcggcgacg aaggagagat cgacgatcat gaagaagccc
     3121 gcgactgcgg cggctagcca gaagctccag ccccacacct cgcgcatggc ctgaaacatc
     3181 aggaccgacg tgaggaacat ggtcagcgcc actgcaatgc cgaatgcgga ggcgagattg
     3241 tccgaagagc gaaagctgac ggcgagcgcc agcgtcagcg tcatcagcgt ccagttgacg
     3301 aagccgacgt agatctggcc gtaaccttgc gaggaggtct gcttgatgta gaggcgcggg
     3361 cataatccga gttgaatggc ctgccttgtc atcgagaagg tgccggagat gatcgcctgg
     3421 ctcgcgataa tggtggcgac ggtcgcgagc gcgaccagcg gcaattgcag gcgctcggga
     3481 cacagaagga aaaacggatt cgatccggat gccgtcttgc cctcgaccag gatcgcggtc
     3541 tggccggcat agttcagaac cagcgtcggc agaacgagcc cgtaccaggt cagccggatc
     3601 gggcgcgcgc caaaatgacc catgtcggcg tagagcgctt ccgcgccggt cgcgcacaaa
     3661 aatacaccgc cgagcaccag gaagccggta aaaccgtgtc cgaagagata ggagagaccg
     3721 tagcgcggat cgagtgcagc gagcaccgac ggatggtcga tgatgccgcc aaggccgagc
     3781 aggccgatcg agatgaacca gatggtcatg atcggtccga acagcgcgcc gatgcgcgcg
     3841 gtgccctgcg gctgcagcgc gaatacggcg atcagcacga ggaccgacag cggcagcacg
     3901 taaggcgcga acgagggcaa ggggtccttc aggccctcca gcgcgctcaa cacggagatc
     3961 gccggcgtga tggctccgtc gccgtaaagc aaagccgcgc cgataatgcc gagcgcgatc
     4021 acgaaggggc gctcgccgtg cttgatgccg atcagcgaca tcagcgcaag aatgccgccc
     4081 tcaccatcgt tatcggcgcg catcacgatc gcgacatact tcagcgaggt ggtgatgatc
     4141 agcgtccaca ccacgagcga caggatgccc agcgcaactg ccggcgtggc ttcgccgccg
     4201 gcccactcga tcgcggtctt gaaggtatag agcgggctcg tgccgatgtc gccatagacg
     4261 acaccgagcg caccgatcgc catggtagcg agcgtaccgt gttccgggcc ggtctccgcc
     4321 ggcgtctccg cgtgcgaggg cgcttgcgcg cgttcaacgg cggtcatgat ccgctcccat
     4381 ggctcgtcag gttccgcgtc gcgggcctgg catcagccgc agtttgcggc gccggctcaa
     4441 tctaggcaat gcgcgccatg ctgagatgac agcgggtagc ttgatgggtt gcggtttcgt
     4501 gaaaaaggaa gcgggtttat tggccgatat tcaccaacag gacgatggtg aggagcaatc
     4561 cgatgcaaaa gagcggagaa taggggcctt gcgcgccctc agaactctcg tgcaaacggc
     4621 ctatccgtcg gcgcgcccgg tgccggacac caatcgtggt cagttgcgca atggcaaatc
     4681 tcatgactgc ctcgaggctt gtttcgggca taatgtgctt gggaggccat tcaaaatcca
     4741 gatcgggcgg cagctcgcat tataaaaatg tcataaagga aagccccggc gaggccgggg
     4801 ctttgggtta ctggtggccc aaagtcgccg acgtcgcgcg aagtgaaata gcgagccgcc
     4861 taatcttgta aatttttgag atgaaagact ccggcactct ggagtagcgg atacaaattt
     4921 ttctgtcttg atttcgattt atcctttttc gtcatcacgt caaggcatca aatcaacgat
     4981 gcaaaatcgt gcgctcgttc gccgtgagcg gatgatgcaa tcgttgcagg agcgaaaaaa
     5041 acgtgcgccc gggcggtgtg acaagcaatc tgtaaggtaa gcgtaagcgc agtgaattac
     5101 agatttgttg cgctgcatca cggagcgctt agagaaacct gtcggtcgac caacgatcgc
     5161 tggaggcagt cgaggtgaat gtgtttggat acctgttgaa acattctcga ttggcggtga
     5221 tcgtcgtcgc cgcgatcggg gccgcagcgc tgctgtggag cagggggttt gtgggccgtg
     5281 ccgacgggca agcgaccagg aaggagttgg ttgcatccgc cagatccgag cagaccggtt
     5341 cgatcggcag cggcgaggaa tcggtcgatc tctctcagaa gcaagcgggt tcgatcaagg
     5401 tcggtacgct tggcgcccgt aaattcgcca ttctgaagac ggccgtcggc acgatcgatt
     5461 tcaacgagaa catgctggtc caggtcttca cccaatatca gggcaagatc atcaaggcgt
     5521 tctataatgt cggcgacgac gtcaagcagg gcgacatcct gttcacaatc gatagccccg
     5581 atctcctgca ggccgagtcg acactgcttg cggccgccgg cgtactcgaa ctccagaaga
     5641 agacgctggc gcgggtgagc ggtttgttga agacgggcgg tgccgcgcaa aaggacgtcg
     5701 atcagtcaac gtcggaccaa cagactgcag aaggcaactt caaggcggcc aaagacgctg
     5761 tgcgcatctt cggcaggctc gacgaagaga tcgatgagat tcttgtcaat cgcagggtga
     5821 actcaacgct gaaggtgccg agcccgatct cgggaaggat cgtgacccgg aacgccgcgc
     5881 cgggtctcct gacccagcca ggcacgccac cgccgccata ccagatggcg gatgtctcaa
     5941 cgatgtggat ggtcgc
//
LOCUS       DATLJT010001642         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2465692,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001642 DATLJT010000000
VERSION     DATLJT010001642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465692"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1755
                     /locus_tag="VM910_37435"
     CDS             106..1755
                     /locus_tag="VM910_37435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVI68224.1"
                     /translation="MSSITTSAIETPSRRSVERTFDDLALCVLLAVGLIAGLTFRDYG
                     LGWDDYTHAEYADLLLKMYGSGFKDTGALSFANLYMYGGGFDMAAALLHKVIPLELFE
                     TRRLLGAIVGVIGLAVTWRLGRRVGGPLAGLVALLLLALCPTFYGHMFMNPKDAPFAV
                     AMAVLMLGLVRLAEEYPQASPRTILIIGLGSGLSIGSRILGGLALVYALIGFLPLFIE
                     EVRKQGARAAIGRFVHVLYTLLPGLVLGYLIMGLIWPWSIIELGNPFHALTYFSHFFE
                     KPWKEMFDGALVSVPDMPWSYLPTLFALQLPEVLLVLSLAGVVGTFIVLPRSDVTARR
                     KTILLMLTLAAAMPIAIAIVKRPALYNGIRHFIFVIPPMTVLAGLTFEWIMRWLKDNR
                     RSWQPAVLAVFVFGLLLPLGEMIRLHPYQYTHFNHLAGTVRGADDRFMLDYWGLAFKQ
                     ASDDLRDQLEEKHEVPPKGRKWKVAVCGPQRPAQVALGPDFTIGWDSHAADFAMTLGE
                     FYCKGLSAPIMVEIKRDDVVFARVYDIRGRSISSLLSIPAP"
     gene            complement(1908..>2217)
                     /locus_tag="VM910_37440"
     CDS             complement(1908..>2217)
                     /locus_tag="VM910_37440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVI68225.1"
                     /translation="VEFGDHSTAPAIALPWNMKPLAAARKALTEEWGKEALLIGSGAS
                     IPIVADFRKTLGLDTLLVGFGLDDDNIHSPNEKYDLKSFHKGIRSWARILAALAELPR
                     "
BASE COUNT          352 a          720 c          676 g          469 t
ORIGIN      
        1 ccataatgcg caattacttg gatttggaac tgagctatag gaaactgggg aacaaggccc
       61 accagcgcca gcggggccag aggggacgct gagttaccaa tagccatgtc atccatcacg
      121 acctctgcca tcgagacgcc ctctcgtcgc tcggtggagc ggactttcga cgacctcgcc
      181 ctttgtgtgc tgctggcggt ggggttgatc gcagggctaa ccttccgcga ctacggcctc
      241 ggctgggatg attacaccca cgccgaatat gccgatctcc tcctcaagat gtacggctcc
      301 ggtttcaagg acaccggcgc gctgtcattt gccaacctct acatgtatgg cggcggcttc
      361 gacatggccg cggcgctgtt gcacaaggtc attccgctcg agctcttcga aacgcgcagg
      421 ctgctcggcg cgatcgtcgg cgtgatcgga cttgcggtga cttggcggct cggccgccgg
      481 gtcggcggac cgctcgcggg tcttgtcgcg cttttgctgc ttgcgctgtg cccgactttc
      541 tacgggcata tgttcatgaa tccgaaggat gcgccgttcg cggttgcgat ggcggtcctg
      601 atgctgggcc tggtgcgcct tgcggaagaa tatccgcagg catcgccgcg gaccattttg
      661 atcatcggcc tcggctccgg cctgtcgatc ggctcgcgca tcctcggcgg actcgccctg
      721 gtctacgcgc tgatcggttt cctgccgctg ttcatcgagg aagtccgcaa gcagggtgcg
      781 cgcgcagcca tcgggcgctt cgtccatgtc ctctacacac tcctgcccgg gctcgtgctc
      841 ggctatctca tcatggggct gatctggccg tggtcgatca tcgagctcgg caatcccttc
      901 cacgcgctca cctacttctc gcacttcttc gaaaagccct ggaaggagat gttcgacggc
      961 gcgctcgtgt ccgtgcccga catgccctgg tcgtatttgc cgacgctgtt cgcgttgcag
     1021 cttccggaag tgctgctggt gctgtcactt gccggcgtcg tcggcacgtt catcgtgctg
     1081 ccgcgcagcg acgtcaccgc gcgccgcaag accatcctgt tgatgctgac gctcgccgcc
     1141 gccatgccga ttgcgattgc gatcgtgaag cggcccgcac tctacaacgg catccggcat
     1201 ttcatcttcg tgattccgcc gatgaccgtg ctggccggtc tcaccttcga atggatcatg
     1261 cggtggctca aggacaatcg gcggagctgg cagccggcgg tgctcgccgt ctttgtattc
     1321 ggcctgctgc tgccgctcgg cgagatgatc cgcctgcatc cctatcagta cacccatttc
     1381 aaccatctcg ccggcacggt gcgcggcgcc gacgaccgct tcatgctgga ttactggggc
     1441 cttgccttca agcaggcttc cgacgatctg cgcgaccagc tcgaggaaaa gcatgaagtg
     1501 ccgccgaagg gccgcaaatg gaaggtggcg gtctgcggcc cgcagcgtcc ggcgcaggtg
     1561 gcgctgggtc ccgatttcac catcggctgg gacagccacg ccgccgactt cgccatgacg
     1621 ctcggcgagt tctactgcaa gggcctgagc gccccgatca tggtcgagat caagcgcgac
     1681 gacgtggtgt tcgcgcgcgt ctacgacatc cgcggccgca gtatttcgag cctgctctcg
     1741 atccccgcac cgtaaggttc cgcaagcgcg accggatcgc tcccgagcga tcggattgaa
     1801 attttgcgat ttcggtgtag agtgctgcgc atacaccctc tacacgtcat ggaatgaaaa
     1861 cgccgcccaa ttccgggcgg cgtttttgct ttgacgacgt gccgggctca gcgcggcaat
     1921 tcggccagcg ccgcgagaat gcgcgcccag gaacgaatcc ccttgtggaa gctcttcagg
     1981 tcgtatttct cgttcggcga atggatgttg tcgtcgtcga gcccgaagcc gaccagcagc
     2041 gtgtccagcc cgagcgtctt cctgaagtcg gcgacaatcg ggatcgatgc cccggacccg
     2101 atcagcagcg cttcctttcc ccattcctcc gtcagcgcct tcctcgccgc ggcgagcggc
     2161 ttcatgttcc aggggagcgc aatcgcgggc gcagtggagt ggtcgccgaa ttcgacc
//
LOCUS       DATLJT010001643         1990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3155542,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001643 DATLJT010000000
VERSION     DATLJT010001643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3155542"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VM910_37445"
     CDS             <1..252
                     /locus_tag="VM910_37445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HVI68226.1"
                     /translation="LPLLQSIGANVLLLTSITEELTKLEGRVDDLHDIGLKELFLKHR
                     NANTMDFIVGAEIYDHLEKVADRFDDVANEINSIVIEQV"
     gene            262..1266
                     /locus_tag="VM910_37450"
     CDS             262..1266
                     /locus_tag="VM910_37450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVI68227.1"
                     /translation="MDATLGVPVLTGLIVVALAFDFLNGLHDAANSIATIVSTRVLRP
                     HYAVFWAAFFNFVAFLVFGLHVAQTIGTGIIEPSIVDAHVIFAALVGAIVWNLITWGL
                     GIPSSSSHALIGGLVGGGMAKAGISAAVWSGLSKTMAAIVLSPLVGFLLAMMLVAIVS
                     WASVRSTPFAVDRAFRILQFASASLYSLGHGGNDAQKTMGIIAVLLFSQGHLGSEFSV
                     PFWVVLACQASMALGTLMGGWRIVRTMGLRITKLTPMQGFCAETGGAATLFMATFLGV
                     PVSTTHTITGAIVGVGAARRVSAVRWNVASSIVYAWVITIPASAIVAALAYWGVLVFR
                     "
     gene            1345..>1990
                     /locus_tag="VM910_37455"
     CDS             1345..>1990
                     /locus_tag="VM910_37455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVI68228.1"
                     /translation="MTDLAVTTDSPSRSPLASEVARRRTFAIISHPDAGKTTLTEKLL
                     LFGGAINLAGQVKAKGERRNTRSDWMKIERERGISVVTSVMTFEFDGLVFNLLDTPGH
                     EDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFNNKMDRESRD
                     TFDLMDEIEKTLALDTSPMTWPLGRGRDFAGTFDIASGQMRLMGEDAEATLAPID"
BASE COUNT          333 a          642 c          604 g          411 t
ORIGIN      
        1 ttgccgctct tgcaatcgat cggcgccaat gtcttgctgc tgacgtcgat caccgaggaa
       61 ttgaccaagc tggaaggccg ggtcgacgat ctccacgaca tcgggctgaa ggagcttttc
      121 ctcaagcacc gcaacgccaa caccatggat ttcatcgtcg gcgccgagat ctatgaccat
      181 ctggaaaaag tcgctgatcg cttcgacgac gtggcgaacg agatcaactc catcgtgata
      241 gaacaggtct gaggacgcgc cgtggatgcc acgcttggtg ttcctgtact gaccggcctg
      301 atcgtggtcg cgcttgcatt cgactttctc aatggcctcc acgacgctgc caattcgatc
      361 gcaaccatcg tctcaacccg cgtgctgcgg ccgcattacg cggtgttctg ggcggcgttc
      421 ttcaatttcg tggcgttcct cgtgttcggc ctccatgtcg cgcagaccat cggcaccggc
      481 atcatcgagc cgtccatcgt cgatgcgcac gtgatttttg cagcgctcgt cggtgccatc
      541 gtctggaacc tgatcacctg gggccttggc attccgtcat cctcgtcgca cgcgctgatc
      601 ggcggcctgg tcggcggcgg catggcgaaa gccggcattt ctgccgcggt ctggagcggg
      661 ctgtcgaaga ccatggccgc gatcgtgctg tcgccgctcg tcggatttct gctggcgatg
      721 atgctggtcg cgatcgtgtc ctgggcctcg gtgcgctcga cgccgtttgc cgttgaccgc
      781 gcctttcgca ttctgcagtt cgcctccgcc tcgctctatt cgctcggcca tggcggcaac
      841 gatgcgcaaa agaccatggg tatcatcgcc gtcctgctct tttcgcaggg tcatctcggc
      901 agcgaattct ccgtgccgtt ctgggtggtg cttgcctgtc aggcctcgat ggcgctcggt
      961 actttgatgg gcggttggcg gatcgtccgc accatgggct taaggatcac aaaattgacg
     1021 ccgatgcagg gcttctgcgc cgagacgggc ggagcagcga cactattcat ggcgaccttt
     1081 ctcggggttc ccgtctcgac cacccatacg atcaccggag ccatcgtcgg cgtcggcgcc
     1141 gcgcggcgcg tctcggcagt gcgctggaac gtggcgagct cgatcgttta tgcctgggtg
     1201 atcacgatcc cggcgtccgc gatcgttgcc gcgctggctt attggggggt gctggtattt
     1261 cgctgacgca aggcgatgcc tatatcggca ggagggggaa atggccttga tcccccgcac
     1321 gttccctgcc aaacgctcac ccccatgacc gacctcgctg tcacgaccga ttcgccgtcc
     1381 cgctcgccgc tcgcctccga agtggcgcga cggcgcacct ttgcgatcat ctcgcatccg
     1441 gacgccggaa agaccacgct gaccgagaaa ctcctgttgt tcggtggcgc catcaatctc
     1501 gcagggcaag tcaaggccaa gggcgagcgg cgcaacaccc gctcggactg gatgaagatc
     1561 gagcgcgagc gcggcatctc ggtcgtcacc tcggtgatga ccttcgagtt cgacggcctc
     1621 gtgttcaacc ttttggacac gcccggtcac gaagactttt ccgaagacac ctaccggacg
     1681 ctgaccgcgg tcgactccgc cgtcatggtg atcgacgccg caaagggcat cgaggcgcgc
     1741 acgcgaaagc tgttcgaggt ctgtcgcttg cgcgacattc ccatcatcac cttcaacaac
     1801 aagatggacc gcgagagccg cgacacgttc gatctcatgg acgagatcga gaagacgctc
     1861 gcgctcgaca cctcgccgat gacctggccg ctcggccgcg gccgcgattt cgccggcaca
     1921 ttcgatatcg ccagcgggca gatgcggctc atgggcgagg acgcggaggc gacgctcgcg
     1981 ccgatcgacc
//
LOCUS       DATLJT010001644         2825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1778234,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001644 DATLJT010000000
VERSION     DATLJT010001644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778234"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1116)
                     /locus_tag="VM910_37460"
     CDS             complement(91..1116)
                     /locus_tag="VM910_37460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HVI68229.1"
                     /translation="MHYQFTLSRPLLRALLASAAIAAQMTLAGCGGGTPATSGRHMQP
                     LSERMLATLKAKNMEKDSPILMRIFKEEAELEVWKQDDSGRFALLRTYPICRWSGELG
                     PKIKTGDRQAPEGFYTITPGLMNPDSSQYLAINTGFPNAYDRANSRSGAFLMIHGGCS
                     SAGCYAMTDEQIAEIYALAREAFFGGQRSFQLQAYPFRMTPLNMARHRNSPHMAFWRM
                     IKEGHDHFEVTRLEPKVDVCEKRYIFDADSADRFSPAERCPAYQVREDIVVAVREKQH
                     RDEMETVELINRGTSTVPVTTGGDGGMNPTFLAAVKSNRDPRAVIRTASGTIPAYVDP
                     PAGDGED"
     gene            1279..1431
                     /locus_tag="VM910_37465"
     CDS             1279..1431
                     /locus_tag="VM910_37465"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI68230.1"
                     /translation="MDKILKGAKPGELPVEQPIRFELAINLRTARTLGIVVSSNVQQL
                     ADEVIE"
     gene            complement(1438..2208)
                     /locus_tag="VM910_37470"
     CDS             complement(1438..2208)
                     /locus_tag="VM910_37470"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVI68231.1"
                     /translation="MPMSLFDLLGRVAIVTGGNGGIGLGMAEGLASAGAIVVLAGRNA
                     KKGETAVQRIAQAGGQAEFTAVDVTSETSCRALIDKAAARHGRLDILVNNAGINIRKS
                     PDTLSAAEWRTVIDTNLTSAFISTHAAYPHMKKAGRGKIINIGSMMSIFGAGFAPAYA
                     ASKGGIVQFTRACATAWAKANIQVNAVLPGWVDTDLTRAARREVAGLNEQVLARTPAG
                     RWGDPKDFAGIAVFLASAASDFVTGAAIPVDGGYSVQG"
     gene            complement(2305..2694)
                     /locus_tag="VM910_37475"
     CDS             complement(2305..2694)
                     /locus_tag="VM910_37475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68232.1"
                     /translation="MKSVILECVLVVMAFAVLEETALARSYLNCLTKKVAIVDAPKGA
                     TSSSIEENFGFWIDEATKIVTLADGKKLNVRRFDERWISAVSGDVSYELDRQSGNLTY
                     AGSTMKDGIATTIIGAGQCTLAAVPAR"
BASE COUNT          519 a          877 c          875 g          554 t
ORIGIN      
        1 taacgacgcg ctatggcggg cgtctcgaag ggtgggggcg gcctcttgcg tcgagacgcg
       61 gccctgcggg ccgctcctca gtccagggaa ttagtcttcg ccatcgcccg ccggcggatc
      121 gacataggcc gggatggtgc ccgaggcggt gcggatcacc gcgcgtggat cgcggttgga
      181 cttcacggcc gcgaggaagg tcggattcat gccgccgtcg ccgccggtcg tgaccggcac
      241 agtggaggtg ccgcggttga tcagctcgac ggtttccatc tcgtcacggt gctgcttctc
      301 gcgtaccgcg accacgatgt cctcccgaac ctggtaggct gggcaacgct cggcggggct
      361 gaacctgtcc gcggagtctg catcgaaaat gtagcgcttc tcgcatacat caaccttcgg
      421 ttcgagacgt gtgacctcga aatgatcatg accctccttg atcattctcc agaacgccat
      481 gtgcggcgag ttgcggtggc gagccatgtt gagcggcgtc atccgaaacg gataggcctg
      541 aagctggaac gatctctggc cgccgaagaa cgcttcgcgg gcgagcgcgt agatttccgc
      601 gatctgctcg tcggtcatgg cgtagcagcc ggccgatgag cagccgccgt ggatcatcag
      661 gaacgcaccg ctgcggctgt tcgcgcgatc gtatgcattc gggaagcccg tattgatcgc
      721 gagatactgg cttgaatcag gattcatcag gccaggggtg atggtgtaaa agccctcggg
      781 cgcctggcgg tcgccggtct tgatcttggg gcccagctcg cccgaccagc ggcagatcgg
      841 atacgtgcgc agaagcgcaa agcggccact gtcgtcctgc ttccaaacct cgagctcagc
      901 ctcctccttg aagatgcgca tgaggatcgg agagtccttc tccatgttct ttgctttgag
      961 cgtggccagc atgcgttccg acagcggttg catgtggcgg ccgctggtcg cgggcgtgcc
     1021 gccgccgcac ccggcaagcg tcatctgcgc agcgatcgcg gccgacgcca ggagcgcacg
     1081 cagcagaggt cgtgaaagag taaattggta gtgcacgcca gccccaatcc cgaacatgac
     1141 ggatgctagg tcgggacttg ggttagatca cggcaaaggc caaccgacca tcaattcagc
     1201 tgcagttggc aaacaaaagc ttaccagact atttctgaag cggaatggcg ccccctggcg
     1261 cggcgtagcg cgatctacgt ggacaagatc ctcaagggcg ccaagcccgg cgagctgccg
     1321 gtggagcagc ccatcaggtt cgagctggcg atcaatctca gaactgccag aacgcttgga
     1381 atcgtcgtgt cgtccaatgt ccaacaactc gccgacgagg tgatcgagtg aagcgcgtta
     1441 gccctgcacg gaatagccgc catcgacggg gatcgccgcg cccgtcacga aatccgaggc
     1501 agcgctcgcc agaaaaaccg cgattccggc gaaatccttc ggatcacccc agcggcccgc
     1561 gggcgtacgc gcgagcacct gctcgtttag gccggccact tcgcggcggg cggcccgcgt
     1621 cagatcggtg tcgacccagc ctggcagcac cgcattgacc tggatgttcg ccttcgccca
     1681 ggcggtcgcg caggcgcgcg tgaactgcac gatgccgccc ttgctcgccg cgtaggcggg
     1741 cgcgaagccc gcgccaaaga tcgacatcat cgatccgata ttgatgatct tgccgcgccc
     1801 cgccttcttc atgtgcggat aggcggcgtg cgtggagatg aacgcgctgg tgagattggt
     1861 gtcgatcacc gtgcgccact cggcggcgct cagtgtgtcg ggcgacttgc ggatgttgat
     1921 gccggcattg ttgacgagaa tatcgagccg gccatgacgc gccgcggcct tgtcgatcag
     1981 cgcacggcaa gaggtttcgc tcgtcacgtc gactgcggtg aattcggctt gcccgccggc
     2041 ctgcgcgatg cgctgcacag cggtttcgcc ctttttggca ttgcgcccgg cgagtaccac
     2101 gatcgcgccg gccgacgcga gaccttccgc catgccgagc ccgatgccgc cattgccgcc
     2161 ggtaacaatt gcgacgcggc ctaaaagatc gaacaagctc attggaattc ccctgatacg
     2221 atgaggtacg tgcgccagtc tacgtgcggc ggtctggtca agcgcggcgt atcgatcggc
     2281 ggcaaatgat ccgaagcggt catcctaccg cgccggaact gccgcgagtg tgcattgacc
     2341 tgccccaatt attgtcgtcg caatgccgtc tttcatcgtt gaaccggcat aggtcagatt
     2401 gccgctttgg cgatcgagct cataggacac atcgccgctc acagcgctga tccagcgctc
     2461 gtcgaagcgg cggacgttga gcttcttgcc gtctgcgagc gtgacaattt ttgtcgcttc
     2521 atcgatccag aagccaaaat tctcttcgat gctcgatgaa gtggctcctt tcggcgcgtc
     2581 cacgatggcc actttttttg ttagacagtt aaggtagctg cgggctaacg cggtctcctc
     2641 caagacggcg aaagccatca caacgagtac gcattctaga ataacgcttt tcattggttc
     2701 tcccgtcgat ttcgatcgac caggagtcaa tgagctctag cacactcgcc tgcctaaatc
     2761 cgcgtcccgc ctatgtgctt gggctctcca agccggtctt ccagccctca gcacgatccc
     2821 gacgc
//
LOCUS       DATLJT010001645         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2007792,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001645 DATLJT010000000
VERSION     DATLJT010001645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007792"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..699
                     /locus_tag="VM910_37480"
     CDS             349..699
                     /locus_tag="VM910_37480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68233.1"
                     /translation="MFPSASFLALAGTLLLTVADKVPALDVESSCRAAAKMGDRLSLD
                     ATLSQCLADENSARDELEKQWTQFPVALRQRCVTTTETGGDPSYVEVLVCLQMGRDAA
                     QMEDPLGGGRQDKK"
     gene            915..2080
                     /locus_tag="VM910_37485"
                     /pseudo
     CDS             915..2080
                     /locus_tag="VM910_37485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            2125..>2441
                     /locus_tag="VM910_37490"
     CDS             2125..>2441
                     /locus_tag="VM910_37490"
                     /EC_number="4.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HVI68234.1"
                     /translation="MKEAEVKARAFAMPLTSPAYPPGPYRFINREFMVITYRTDPDKL
                     RAVVPEPLEVDEPLVKYEFIRMPDSTGFGDYTETGQVIPVRFRGRKGGYSHCMFLNDE
                     PPIA"
BASE COUNT          481 a          785 c          754 g          421 t
ORIGIN      
        1 acgaattggt gggtgcacga actcaaggag cccaagcgcg gcacctacgt gttcaccgcc
       61 gcggtaagct tccaaaaagg cgagaagggc tattacgccg tgctcaacag ctgcaagctc
      121 agtccgccat cttcgccccc gctgcgatag cacgccacgc atggcgttcg accaaaagcg
      181 ccgagtgtgc gtcgtttgcg cggcgaggcg acagcgctgc gcgatgctct aatttgatgc
      241 aaccgagccc gttgaccggc ctgtgccagc gggctatgcc ccgctgccgg ccgcctcggc
      301 gcacaatttc gattgcgcag ccggcgtgcg agaagacggg agacccgcat gtttccatct
      361 gcgtcgtttc tcgcgctcgc tggcacgttg ctgttgaccg tcgccgacaa ggtccccgcc
      421 ctcgacgtcg aatccagctg ccgtgcggcg gcgaaaatgg gcgacaggct gagcctggat
      481 gcgacgctga gccagtgcct ggcggacgag aacagcgcgc gtgacgagct ggaaaagcag
      541 tggacgcagt ttccggtggc gctgcgtcag cgttgcgtga ccaccaccga gaccggcggc
      601 gatccaagct acgtggaagt tctcgtctgc ctgcagatgg ggcgcgacgc ggcgcagatg
      661 gaagatcctt tggggggtgg tcggcaagac aagaaatgac acaaatcatg cacgtattcg
      721 tgatttgatc ggccgatcat caatccgctg tacctgtcga actcgagagc gctggaacgt
      781 gaaaaacgga tcgtcgctgc atgacctcca ctgatgctcc ggcccgcgtc gcgccttcaa
      841 ccgcgaagcg cgagtcctca cccgtcgatg ccaaaccgtc gacgcggtcg cccaaagatc
      901 aaagccggcc cttcatcaag ccgctcctga agagccaccg cgaactcaag cggccgccgt
      961 tcgactgcat cgccttggta ttgcaaggtg gcggcgcgac tcggcgcctt ccaagccggc
     1021 gtgtatcagg ggctcgccga ggcaaacctg catcccgaat gggtcgccgg aatttcgatt
     1081 ggcgcgatca atgcggcctt gatcgccggc aacgccccgg aggcacggct cgaaaagttg
     1141 cgcgccttct gggagcaggt cacgacccag ctgctgttcg atcccttcgg catcgcccat
     1201 tactggttgc gcggcgatac cggtcacgtc ttcctcaacc gattccgcgc cggagcgacc
     1261 ctgctcgaag gggctcccgg cttttttcaa gccgcgaatt ccgccgcctt acttcgcggc
     1321 gccgggtacg gtcgaggcca ccagctggta cgacaccgcg ccgttgcgca gcacgctgga
     1381 gcgcctcgtc gacttcgatc gcatcaacgc cggcgagatg cgcttcagcg tcggcgccgt
     1441 caacatccgc agcggcaact tcgtctattt cgattcgacc agccacaaga tcgggcccga
     1501 gcacatcatg gcgagcggcg cactgccgcc cggctgtccg gccgtcgagg tggacggcga
     1561 actctattgg gatggcgggc tcgtctccaa caccccgctg caatgggtgg tggcgggcga
     1621 tacccgccag gatacgctca cgttccaggt cgacctgtgg agtgcgcgcg gggacttgcc
     1681 gagcgatctc aatgatgtca gcgcgagaca gaaagagatc caatattcga gcaggacgcg
     1741 ggccaatacg gatcacttca agcagatcca gtgcgtgcgg gtggcattgg ccaacgtgat
     1801 ggaaacgatt ccgcgcgaaa tcctcgactc cgaggacggc aagctgctgc agtccgtcgc
     1861 ggatcgcaac gtctaccaga tcgtgcacct catttatcgc tcgaagaact atgaggttca
     1921 gtcgaaggac tacgagttct cccgcaacag catgatcgag cactggaacg cgggatatag
     1981 cgacgcgacc cgcacgttgc gccatccgga agcgctgcaa cggccgaccg atcagaccgg
     2041 ggtcgccaca ttcgacgtcg ccatcgacgg ccgcgaatga ggcggcgcga tcccgctact
     2101 cccacaaatc agaaggagca cagcatgaag gaagcagagg tcaaggcgcg ggcattcgcc
     2161 atgccgctga cgagcccggc ctatccgccg ggcccttatc gtttcatcaa ccgggaattc
     2221 atggtcatca cctatcgaac ggatccggac aagctgcgcg ccgtggtgcc ggagccgctc
     2281 gaggtggacg agccgctggt caagtatgag ttcatccgta tgccggactc caccggcttc
     2341 ggcgactata ccgaaaccgg acaggtcatt ccggtgcggt ttcgcggccg caagggcggc
     2401 tacagccact gcatgttcct caacgacgag ccgccgatcg c
//
LOCUS       DATLJT010001646         1733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_855566,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001646 DATLJT010000000
VERSION     DATLJT010001646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855566"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VM910_37495"
     CDS             complement(<1..430)
                     /locus_tag="VM910_37495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68235.1"
                     /translation="MSASTADSACVSLPWRATFAPSRTNSAALTFYRDLLGFRITDFM
                     GPPVSLYFMHVNTRHYSLAIAQGPRSRMHHLMMEFYSLDDVGQSYDIAQREDGVAVKF
                     GRHPNDFMTSFYMRTPSDFLIEHGWGGREVSPGWQPVELKS"
     gene            360..1040
                     /locus_tag="VM910_37500"
     CDS             360..1040
                     /locus_tag="VM910_37500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68236.1"
                     /translation="MREGAKVALHGRDTQALSAVLADIAQAGGQASYVTGDTTKFSDI
                     EAMRRRIEREFGPVDILVANAGGSFTTPGPIEQTSEEEWRASIDGNLTATFLTIKSFL
                     PRMKEGGTGTIITISSAAARRPYAMAPIAYAAAKAGIEILTQDLAGQVGPYGIRANCI
                     APETILTERNKTRIPSTQQDDLIELHPIKRLGTPEDVAHAALYLASDDAAWIAGVVLD
                     VAGGAVMV"
     gene            1296..>1733
                     /locus_tag="VM910_37505"
     CDS             1296..>1733
                     /locus_tag="VM910_37505"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVI68237.1"
                     /translation="MMFHPRVARTTILATGSALALITAALGDHAHAEGKLDASYTISF
                     ARIRVGGITATGVFGESQYTISARGHAGGIMKVLVDGAASFSTHGTIKDGYLVPTTFT
                     SKIVSDAETSDVTMVLDEGSVKELAAASPPKSDRVPVTQANRQG"
BASE COUNT          374 a          520 c          516 g          323 t
ORIGIN      
        1 cggatttcag ctcgaccggc tgccagccgg ggctgacttc gcggccaccc catccatgct
       61 cgatcaggaa gtccgaaggc gtgcgcatgt agaatgacgt catgaaatcg ttcggatgcc
      121 gaccgaactt caccgcgaca ccgtcttccc gctgggcaat atcgtagctc tgaccaacat
      181 cgtcgagaga gtaaaattcc atcattagat ggtgcatgcg gctgcgtggc ccttgggcga
      241 tggcgaggct ataatggcgg gtgttgacgt gcatgaaata cagcgacacc ggtggcccca
      301 tgaagtcagt gatccggaag cccagcaggt cccgatagaa tgtaagggcc gcgctgttcg
      361 tgcgcgaagg cgcgaaggtg gccctccacg gcagggatac gcaagcgctg tccgcagtgc
      421 tggctgacat cgcgcaggct ggcgggcaag ccagctatgt tacgggagac acgaccaagt
      481 tcagcgatat cgaagccatg cgccgccgga tcgaacggga attcgggccg gtcgacatcc
      541 tcgtcgccaa tgccggcggc agcttcacca ctcccgggcc catcgagcag acgagcgagg
      601 aggagtggcg cgcatcgatc gacggcaatc tgaccgcgac tttcctcacg atcaaaagct
      661 ttctgccccg catgaaggag ggcggaaccg ggaccatcat tacgatttca tcggccgccg
      721 cacgcaggcc atatgccatg gccccaattg catatgccgc cgccaaggcc ggtatagaaa
      781 ttctcacgca ggaccttgcc ggccaggtcg gtccatacgg aattcgggcg aactgcattg
      841 ctcccgaaac aatccttacc gaacgaaaca agacgcgcat cccttcgact cagcaggacg
      901 atctcattga attgcatccg atcaagcgcc tcgggacgcc tgaggacgtc gcacacgcgg
      961 cgctctatct cgcttccgat gacgcagcgt ggatcgctgg cgttgtcctc gacgtcgccg
     1021 gcggagcggt catggtctga agccgcgcgg cactcgctgg tcgctcggat cgacgacgct
     1081 cggcaacccg gaattcgctg ttccaccctc tgaccgatag gtcacggtgc gaaggcaaga
     1141 agaagtctcg ccggaaacgg cccgataagg ccgttaccaa atgaatggca ggaggcgata
     1201 cttcacgttt gcctgatagt cggtatagcc tggaaggttc tagagccctt gaacggcgcg
     1261 gcagcccgat gtagcgctca aaggacggat caaagatgat gtttcatccg cgcgttgctc
     1321 gaacgaccat tctcgccaca ggctccgcac tcgcgctcat tacggcggcg ctcggcgatc
     1381 atgcacatgc tgaaggcaaa ctcgacgcga gctatacgat ctcgtttgcg cgcattcgag
     1441 taggcggtat cactgcgact ggtgtcttcg gggaaagcca atacacgatt tctgcgcgtg
     1501 gccacgctgg tggcatcatg aaagtattag tggatggagc tgcctccttc tccacgcacg
     1561 gcaccatcaa ggacggttat ctcgtgccga caactttcac ctccaagatc gtctcggatg
     1621 ccgaaacctc ggatgtgaca atggtcctcg atgagggcag tgtgaaggaa cttgcggccg
     1681 catcgccgcc gaagtcagac cgcgtgccgg tgacccaggc taatcgtcaa ggt
//
LOCUS       DATLJT010001647         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1318905,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001647 DATLJT010000000
VERSION     DATLJT010001647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318905"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1776
                     /gene="istA"
                     /locus_tag="VM910_37510"
     CDS             241..1776
                     /gene="istA"
                     /locus_tag="VM910_37510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVI68238.1"
                     /translation="MPGQRLPMRKIRDVLRLRAGGTSKRKIAASLSIGATAAGDCVRR
                     ARRAGLSWPLPENLSDEALERLLYPPPLVAAKDRRAQPDWAEIHRELRRPGVTLQLLW
                     EEHRGIHPDGYGYSRYCELYRAWEGRLSPTMRQQHVAGERMFVDYAGTTLEVVDGATG
                     EVRVCQLFVAVLGASNYTYAEATFTQRLVDWIGSHVRTFTFFNGVTAQIVSDNLRSGV
                     TKACFYEPAVNRTYAEMAHHYGTAVVPARPRRPKDKSKVEVAVQVTTRWVIAKLRNRR
                     FFSLVELNAAIRELVTQLNDRVTRHLGTSRRALFDEIERPALKKLPVEPYLYAEWKEC
                     RVGLDYHVEVERHYYSVPHTLLREKVWARITARTIEVFHRGNRVAAHMRSSSDRRHTT
                     VREHMPSSHRRYADWTPERIRRQAGEIGRNTAALIETIMRERSHPEQGFRAAVGIIRL
                     VKSYGRDRLEAACSRALEIGARSFTSVNSILKNNLEAKRPAPAADGPPIAHSNIRGSR
                     YFH"
     gene            1787..2506
                     /gene="istB"
                     /locus_tag="VM910_37515"
                     /pseudo
     CDS             1787..2506
                     /gene="istB"
                     /locus_tag="VM910_37515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(2646..>3014)
                     /locus_tag="VM910_37520"
     CDS             complement(2646..>3014)
                     /locus_tag="VM910_37520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68239.1"
                     /translation="DKVAEKINAMAGRVVAAAHHSEHKLTSSEMAGFDVLVITHQAWA
                     YRRSRPPIPTDRDHLFRMIATSSEARVLTAPLDDGGDVSLLGVGQARRGAVPVVNRRD
                     PRTTRRALTADAAARAGGPC"
BASE COUNT          562 a          989 c          938 g          525 t
ORIGIN      
        1 ggactgctgc aacgaccggg tttgttctgc tgattacccc tccggcacgc cggccatgcg
       61 catgcctggg cgcaccggct aattgcgacc accgattacg attgattgcg accatttgtt
      121 ccgattgatc gcgaccaccc atccgggcga tcgcgaccag gctgtggacg ggtgtgacga
      181 caccgttggg tgatctgcga atcggcaatg cctcggacct ctttcaagag ggagcggggc
      241 atgcctggac agagattacc catgcgcaag atacgcgacg tgctacggct acgtgccggt
      301 ggcacgtcca aacggaagat tgcggccagc ctatcgatcg gtgcgacggc tgcaggcgac
      361 tgcgttcggc gtgcgcggcg ggccggcctg agttggccgc tgcccgagaa tctgagcgat
      421 gaggcgctgg agcggctttt gtacccgccg ccgcttgtcg cagcgaagga tcgccgagca
      481 cagcccgact gggcggagat ccatcgcgag ctgcgccggc cgggcgtgac gctgcagctg
      541 ttgtgggagg agcatcgcgg catccacccc gacggttacg gctacagccg atactgcgag
      601 ctttaccgcg cctgggaggg gcgcctttcg ccgaccatgc ggcagcagca tgtcgccggc
      661 gagcggatgt tcgtggacta cgccggaacg acgcttgagg tggtggacgg ggcgaccggc
      721 gaggtgcgcg tctgccagct gttcgtcgcc gtgctcggcg cgtccaacta cacctatgcg
      781 gaagccacct ttacgcagcg ccttgtcgac tggatcggct cgcacgtacg taccttcacg
      841 ttcttcaacg gcgtcacagc gcagatcgtt tccgacaact tgaggtcggg cgttacgaag
      901 gcgtgcttct acgagccggc ggtgaaccgc acatatgccg agatggcgca tcactacggc
      961 accgcagtag ttccagcccg tccgcggcgc ccgaaggata aatctaaagt tgaggttgcg
     1021 gttcaagtta cgactcgttg ggtcatcgcg aagctgcgca atcggcgctt tttctcgctg
     1081 gtcgagctca acgccgccat ccgggaactg gtcacgcagc tcaacgaccg cgtgacgcgg
     1141 catctgggga ccagccggcg tgcgctgttc gatgagatcg agcgccccgc gctcaagaag
     1201 ctgccggtcg agccctatct ttacgcggaa tggaaagaat gcagggtcgg cctcgattac
     1261 cacgtcgaag tcgagcggca ttattactcc gtgccgcaca cgttgctgcg cgagaaggtg
     1321 tgggcacgga tcacggcgcg caccatcgag gtcttccatc gcggcaatcg ggtcgcggct
     1381 catatgcgat cgtcatcgga ccggcggcac accacggtgc gtgagcacat gccgagcagc
     1441 catcggcgct atgccgactg gacgccggag cggatcagac ggcaggccgg cgagatcggg
     1501 cgcaataccg cggcgttgat cgagaccatc atgcgcgagc gctctcaccc ggagcaagga
     1561 ttccgggccg cggtcggcat catccggctg gtcaagagct atggccgcga ccggctggag
     1621 gcagcctgca gccgggcgct ggagatcggc gcgcgctcct tcacttccgt caattcgatc
     1681 ctgaagaaca acctcgaagc caaacggccc gcacctgccg cggacgggcc gccgattgca
     1741 cactccaaca tccgtggctc ccgttacttc cattgaggag aacacaatgc tcacccatcc
     1801 cacccttgac cagctcaagg cgttgaatct cgatggcatg gccgatgcct tcgtcgagtt
     1861 gcagaaccga gctcaagccg ccgagctctc acatgccgaa tggctggcgc tgttgctcga
     1921 tcgcgaggcg gccaatcgca acaccaggcg ctttcaaatc cggctgcgca gcgccaagct
     1981 gcgccacagc caagcggcca tcgaggacgt cgactaccgc acgccgcgac ggctcgacaa
     2041 ggcgctgttc cagcagctca cggcaggcaa atggatcgcc gagcaccgca atttgctcgt
     2101 aacggggccg tgcggtatcg gcaaatcgtg gttagcctgc gcactcgccc agaaggcatg
     2161 tcgggacggc tacgcggtgg tctacgctcg cgtgccgcgg ttgttcgccg atctggaact
     2221 cgcccacggt gatggtcgct tcacgcgcct gtttcgtacg ctggtcaagg ccgacttgtt
     2281 gatcctcgac gactggggac cggaccgact caccgccaac cagcggcgcg atctcatgga
     2341 aatcgtcgag gaccgccacg gcaacggctc cacgcttatc accagccaac ttccccgcga
     2401 caagtggcac gatgtcatcg gcgagccgac gttcgcggac gccatcctcg atcgcatcgt
     2461 ccacaatgcc tatcggctcg agttagacgg gccatcaatg cgcaagctcc aagccaacgc
     2521 acgcgccgaa cccgagccgc aacccgcgaa caaagcggcc ggaagcgtaa cttcacacgt
     2581 caccaaacca gcaaaaggag aacagaaatg accgccgtgc ccaacttccc gcccgccccg
     2641 gtgggtcaac agggacctcc cgcgcgcgcc gccgcgtcag ccgtgagggc acgccgcgtc
     2701 gtgcgcgggt ccctcctatt gactaccggg acggcccctc gccgtgcttg accaactccc
     2761 aggagactga catcaccacc gtcatccaac ggcgccgtca gcacccgcgc ctcactgctg
     2821 gtcgcgatca ttcggaacag gtggtcgcgg tccgtcggaa tgggtggtcg cgatcgtcgg
     2881 tatgcccagg cttggtgagt gatcacgagc acgtcgaacc ctgccatttc gcttgaggtt
     2941 agcttgtgct cgctgtgatg agctgcggcg acaacacgcc ccgccattgc attgatcttt
     3001 tctgcgacct tgtc
//
LOCUS       DATLJT010001648         1613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3156511,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001648 DATLJT010000000
VERSION     DATLJT010001648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3156511"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /gene="rplJ"
                     /locus_tag="VM910_37525"
     CDS             complement(<1..204)
                     /gene="rplJ"
                     /locus_tag="VM910_37525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HVI68240.1"
                     /translation="MDRAAKKELVTALNGVFKDTGVVVVAHYSGLNVAQMQTLRRQMK
                     HAGAAVKVAKNRLAKIALEGTDAA"
     gene            786..1316
                     /locus_tag="VM910_37530"
     CDS             786..1316
                     /locus_tag="VM910_37530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68241.1"
                     /translation="MQQRLGVTIAALVAAALVHVSSAAMAQGAGHSDVRQIELTEKQV
                     QGVIAAQKDVAAIFDKLQGPPPDELPPKVLAELDAAAKRHGFKDFNDYDEVVGNITMV
                     MTGIDPKTKAFTEPAAAIQKEIADVTADKTIPAQDKKQMLEELNEALKSAQPIQFPGN
                     IELVKKYYDKIDAGLN"
BASE COUNT          340 a          518 c          481 g          274 t
ORIGIN      
        1 ggctgcatcc gtgccttcga gcgcgatctt ggcgaggcgg ttcttcgcga ccttcaccgc
       61 cgcgcccgcg tgcttcatct gcctgcgcag cgtctgcatc tgggcgacgt tgaggccgga
      121 gtaatgagcc acgaccacga cgccggtgtc tttgaagaca ccgttgagcg ccgtgacgag
      181 ctctttctta gccgctcgat ccactgcaag ctctttccgg ttggcggccc gtcttcacat
      241 ccggaaaccg ccggttgcga cctgccgtcc tgaccttgcg cttaacaaac gccggcagga
      301 cagcgctgcg gaaatcctgc cccccaggcg cgggcgtacg ccgcaccggg ttctcgaggt
      361 tcgaaccggt cgcggggctc tcggccccaa aacggtcttc cccgtctgtg caggcggctg
      421 gcattaagcc gggatccggc acctgcagtc tcggacagga tggacttccg gaaactgccg
      481 gaagaccctg atgcgccaag aaatttcgcg gttggcctcc gctcctttca gataaatccc
      541 gcaggatgtc caaaaggaat gaggtcctga ccgccatgat ttccttgaag cgcgtaaagg
      601 cgcgccgaca aactgggccg gcacgcccta gacctcctat gtagaggttt ccacgggtat
      661 tgcaagcaat aatcacgccg attgcccctg cagggccacc ggaggtccaa ttttcgtcat
      721 gggcgagcgc tagcaaaacc gtcgtcgcga ctcgaatgtc gagccggcaa gcgggggctt
      781 tggggatgca gcagagatta ggtgtgacca tcgcggctct cgtggccgcc gcgctcgttc
      841 acgtctccag tgctgccatg gcccagggtg cggggcactc cgatgtgagg caaatcgagc
      901 tcaccgagaa gcaggtccag ggcgtgatcg ccgcgcagaa ggatgtggcg gccatcttcg
      961 acaagcttca ggggccgccg ccggacgagt tgccgccgaa agtcctcgcc gagcttgacg
     1021 ccgccgccaa gaggcacggc ttcaaggatt tcaacgacta tgacgaagtg gtcggcaaca
     1081 tcaccatggt gatgaccggc atcgacccca aaaccaaggc attcaccgaa cccgcggccg
     1141 cgatccaaaa agagattgcc gacgtcaccg ccgacaagac aattccagcg caggacaaga
     1201 aacaaatgct ggaagaactc aacgaggcgc tgaagtcggc gcagcccatt caatttccgg
     1261 gcaacatcga gctcgtgaaa aagtactacg acaagatcga tgcggggctt aactagcgcc
     1321 ccatcattct ctctcgcttt ggtagaacca aggacgagcg caagcctccg ccgatttgct
     1381 ccgccgccgc gctcacccgt tgctgcccgg acggtccgcg agtgataaga aggcgggcac
     1441 gcaagggaga ccgcgaggga gcaaaatggc agcgagcgcc aaccgcatcc tcaccaccca
     1501 cgtgggcagc ctggtgcggc ccccgcagct gatcgagttc ctgcacaaga tcgaggatcg
     1561 agccccctac gacaaaaccg cctacgaggc ctgtctgaag gacgcgatcg aca
//
LOCUS       DATLJT010001649         1947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1779861,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001649 DATLJT010000000
VERSION     DATLJT010001649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779861"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VM910_37535"
     CDS             <1..117
                     /locus_tag="VM910_37535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVI68242.1"
                     /translation="QLKAAGVGEELVRLSVGIEAAADIIDDLASALRASQKV"
     gene            121..903
                     /locus_tag="VM910_37540"
     CDS             121..903
                     /locus_tag="VM910_37540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68243.1"
                     /translation="MNVTVNGVRAFVSTGGRDFDPSLPSVVLIHGAGFDHSTWALHSR
                     YFAHHGFSMLAPDLPGHGRSAGPALRTIAEMADWIAALLAATGATKAHLIGHSMGSLI
                     ALEAAARHPDKVSALSLIGTAAPMTVGPDLLKAAEANDQAAIDMISIWGLGFSAELGG
                     SLAPGLWMHGSAQRVLQGCAPGVLFSDLSACNSYVDALAAAAKVKVPTTVILGERDMM
                     TPPRAGKTLAAAIAGARTVVVPGAGHMLMNERPDELLAALRG"
     gene            complement(1042..1164)
                     /locus_tag="VM910_37545"
     CDS             complement(1042..1164)
                     /locus_tag="VM910_37545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68244.1"
                     /translation="MTLDTIMVMIAVTMMFATLAIVLGWADRRTNGAVKSPVKR"
     gene            1321..1737
                     /locus_tag="VM910_37550"
     CDS             1321..1737
                     /locus_tag="VM910_37550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HVI68245.1"
                     /translation="MRATIFAVLLLAAVLGFASPARAADDIAAAQGVIRSQEQAFSRG
                     DAAAAHSYAAPAIRQLFPEASIFMSMVQHSYAPVYRHRSFDFGEAKSDGDLIAQRVHI
                     VDENGDGWEALYTLERQADGTLKITGCTLLKSGQSA"
     gene            complement(1744..>1947)
                     /locus_tag="VM910_37555"
     CDS             complement(1744..>1947)
                     /locus_tag="VM910_37555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI68246.1"
                     /translation="SEAATRLGIVIFASVIGMSFGGWVSGVIFDATGSYAAAFANGLA
                     WNALNIAIMLGLLLRARARLAMA"
BASE COUNT          334 a          638 c          632 g          343 t
ORIGIN      
        1 caattgaagg ccgcgggcgt cggggaagag ctggtgcggc tttccgtcgg catcgaagcg
       61 gcagccgaca tcatcgacga tctcgcaagc gcgctgcgcg cctcgcaaaa ggtttgagcc
      121 atgaatgtca ccgtcaacgg cgtgcgcgcc tttgtttcga ccggcggccg cgatttcgat
      181 ccgtcgctcc cttccgtcgt cctgatccac ggcgcgggct tcgatcattc gacctgggcg
      241 ctgcacagcc gctattttgc ccatcacggc ttctcgatgc tggcaccgga cctgccgggt
      301 catggacgct cggcaggacc cgcgctgcgc acgatcgccg agatggccga ctggatcgcg
      361 gccttgctcg ctgcaacggg agcgaccaag gcgcatctga tcggccattc catgggctcg
      421 ctgattgcgc tggaggccgc ggcgcggcac ccggacaagg tgtcagcact cagcctgatc
      481 ggaacggcag cgccgatgac cgtcggcccc gatcttctca aggcggccga agccaacgat
      541 caggcggcga tcgacatgat ctcgatctgg ggactggggt ttagcgccga actcggcgga
      601 agcctggcgc cagggctctg gatgcatggc agcgcgcaac gcgtgctgca gggttgcgcg
      661 cccggcgtgt tgttctcaga tctttcggcc tgcaattcct atgtggacgc gctggccgcg
      721 gcagcgaaag tcaaggtgcc gacgacagtg attctcggtg agcgcgacat gatgaccccg
      781 ccgcgcgccg gcaagacgct ggcggcagca attgccggcg cacgcacggt ggtggtgccc
      841 ggcgccggcc acatgctcat gaatgagcgg ccggatgagc tgctggcggc actgaggggg
      901 tgagccgact ctgcgcggcg caatttccgg gggatagcgc ctgcgccctg tgttcgcccg
      961 ataccgacga ggccggcgat tggcactcct ccgcgattcc agggccgcgt caccagatgc
     1021 ctccgggatt ggccgctggc cctatctttt caccgggctc tttaccgcgc cgttggtgcg
     1081 cctgtcggcc cagcccagaa cgatcgcgag cgtcgcaaac atcatcgtga cggcgatcat
     1141 caccataatc gtgtcgaggg tcatcgtcct tctccatgat ccatggaaaa gaacttaacc
     1201 cgcggacaag agtacgtttt tgcgctggat caaaaacgcg aaacgtcggg ccgacgcgcg
     1261 ttgcggctgc cgccgcgaag gcttacggtt gcttgcgatc gagactttgg aatgtcgagc
     1321 atgcgcgcca ccattttcgc tgtcctgctg ctcgccgccg tccttggctt cgcaagcccg
     1381 gcccgcgcgg ccgacgacat cgcagccgcg cagggcgtga tccgctcgca ggaacaggcg
     1441 ttttcccgcg gcgacgccgc ggccgcccat tcctacgccg cacccgccat ccgccagctt
     1501 tttccggaag cctcgatctt catgtcgatg gtgcagcact cctacgcgcc ggtctatcgg
     1561 caccggagtt ttgatttcgg cgaagcgaag tccgacggcg acctcatcgc ccagcgcgtg
     1621 cacatcgtcg acgagaacgg cgacggctgg gaagcactgt atacgctgga gcgacaggcc
     1681 gacggtactc tgaagatcac cggctgcacg ctgttgaagt ccgggcaatc ggcttgacgc
     1741 gggctacgcc atggcgagcc gcgcccgcgc acgcagcaat agtccgagca tgatcgcgat
     1801 gttcaacgcg ttccaggcaa gcccgttggc gaaggccgcg gcataggatc cggtggcatc
     1861 gaagatcacg cccgagaccc agccgccgaa cgacatcccg atcacggagg cgaagatcac
     1921 gatgccgagg cgggttgcgg cttcgga
//
LOCUS       DATLJT010001650         1797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3848207,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001650 DATLJT010000000
VERSION     DATLJT010001650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848207"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VM910_37560"
     CDS             complement(<1..345)
                     /locus_tag="VM910_37560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI68247.1"
                     /translation="MTTEVGLQKDGEIAVITVDNPPVNALKQVVRAGLLSTLTQARDD
                     AATRAIVLVGAGRTFIAGADITEFGKPSQPPSLPEIIGLIENSPKPVIAAMHGTPLGG
                     GLELTLACHFRIA"
     gene            691..1644
                     /locus_tag="VM910_37565"
     CDS             691..1644
                     /locus_tag="VM910_37565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68248.1"
                     /translation="MPLAARAQQPAMPVIGYLSARSPEDTAHLVEAFRRGLGEAGFFE
                     GHNVTIEYGWALGQADRLPELAADLVRKPVSVLVSTGGESAALAAKAATSTIPIAFII
                     GGDPVKLGLAASYRRPGGNATGISILTATLEPKRLELLRELVPHVSTIGALLDPNFRP
                     YEGQLRDLREAARTLDLQVEELRASTDVEIDLAFETLARQRITALTVVAGPFFDTRRE
                     KLVTLAARHAVPTMYHFREFPVAGGLISYGIDSRVTYRHIGVYAGGILKGEKPAELPV
                     LQPTKFELVINLKTAKTHGAAAGQHLALLAQPDRSRPRKVL"
BASE COUNT          339 a          552 c          572 g          334 t
ORIGIN      
        1 cgcaatacga aaatgacacg cgagcgtcag ctcgagcccg cccccgagcg gggttccatg
       61 catggccgcg atcaccggct tcggcgaatt ctcgatcagc ccgatgatct cgggcaggct
      121 cggcggctgc gacggcttgc cgaactcggt gatgtcggcg ccggcgatga aggtgcgacc
      181 cgcgcccacg agcacgatcg cgcgcgtggc cgcgtcgtcg cgcgcttgcg tcagcgtgct
      241 caacaggccg gcgcgcacga cctgcttgag cgcgttgacc ggcggattgt cgacggtgat
      301 gacggcgatc tcaccgtcct tttgtaagcc gacttctgtg gtcacaggca gtcagcctcc
      361 ccgttggtgc gacagcgatc gccgcagtgc ggaatcgatc ttcgcgcgcg gcgctggcgc
      421 attccacagc ggaacttcgt ctaattccgc agtgcggaaa ctgattttgc aatttgacgg
      481 cgacgctagt acccggaatc actggttcgc gaaacgtgct ttaagccgtt tctggtggac
      541 ctcggatttg atccaagcga acggcttggc gcgttcatta tagtccccga tgaaggcgtc
      601 gatatgggaa cgcaattgag gaagcacggg aggcatcggg cagtgagacg ccgcgagttt
      661 atcacgcttc tcggtggcgc ggcggcagcg ttgccgctcg cggcgcgcgc gcagcagccg
      721 gcgatgccgg tgatcgggta tctcagcgct aggtcccccg aggataccgc gcacctggtg
      781 gaggcatttc gacgaggcct gggcgaagcg ggcttcttcg agggccataa tgtgacgatt
      841 gaatacggtt gggcactcgg tcaggccgac cgactgccgg agcttgccgc agacctggta
      901 cgcaagcccg tgtccgttct cgtttccacg ggtggggaat cggcggcact ggcggccaag
      961 gccgcgacct cgaccatccc gatcgcattc atcatcggcg gcgatccggt caagctgggg
     1021 ctcgcagcga gctacagacg gccgggcgga aacgccaccg ggataagtat cttgaccgcc
     1081 acgttggaac ccaagcggct cgaactgttg cgcgagctgg tgccgcacgt ctctacgatc
     1141 ggcgcccttt tggacccgaa cttccggccc tatgaaggcc agttacgcga tctgcgcgag
     1201 gcggcgcgca ccctcgactt gcaggtcgaa gaattgcgtg ccagcacgga tgtggagata
     1261 gacctagcct ttgaaacgct tgcgcggcag cgtatcaccg ccctcacggt cgtcgccgga
     1321 ccgttttttg acacacggcg cgaaaagctt gtgaccttgg cggcccgcca tgcggtgccg
     1381 acgatgtacc atttccgcga attccctgtg gccggagggc taataagcta cgggatcgat
     1441 tctcgcgtta cctatcgcca tatcggagtt tacgcgggcg ggattctcaa gggtgaaaag
     1501 ccggccgaat tgccagtcct gcagccaacc aagttcgagt tggtcatcaa tttgaagaca
     1561 gcaaagacgc acggtgcggc tgccgggcaa cacctagctc tgttagccca gccagaccgc
     1621 agccggcccc gcaaagtgct ctaggttttt gggagcgcgg cgctcaaaat cgcgggaagg
     1681 aacgccatct cgtcgacgcc agccggcagc ctggcgatga gctgtttcaa gcggtcgtcc
     1741 aataacagca tcgcaaaacc gtgtaccaac gaccatgcga ccaccatttg agcagcc
//
LOCUS       DATLJT010001651         1709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1320022,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001651 DATLJT010000000
VERSION     DATLJT010001651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320022"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VM910_37570"
     CDS             <1..235
                     /locus_tag="VM910_37570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HVI68249.1"
                     /translation="YYIMAWRAGKTALLQSSLTRAVVFSAMTTATAFGSLWLSNHPGT
                     SSMGKLMALALVCTMAAAVLFQPALMGPPREKP"
     gene            complement(431..>1709)
                     /locus_tag="VM910_37575"
     CDS             complement(431..>1709)
                     /locus_tag="VM910_37575"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HVI68250.1"
                     /translation="TKDRLYRGLDHLLAHKAALEAHLSQRCGELFAIENEVLLYDVTS
                     TYFEGQAEANPQAQRGYSRDHRPDCKQVLIALVVTFDGFPLGYEVFAGNTHDARTLQT
                     IVATMEARHGMLGRVWITDRGMASAQNLAWLRQTGRRYIIGAPKSELKKFGCDLAHPD
                     GWRTVQEGVEVKLTRHPETDETVILCRSADRRSKEKAMHDKFSRRIEAALERLAARVV
                     RSRKRLDPAVVNRQIGRILQQNQRAAARFTIVLEPDSCPAGFRVRVTCNAAFDDWAAL
                     SEGAYLLRSNIADWSDQQLWKAYIQLTQAEAAFRIQKDQLKVRPIWHQREDRVQAHIL
                     VCFLAFVLWKSLEMWQQRAGLGNSPRTILEELARIQSHDVVLPTATHGQIRLRCVTQP
                     DPAQAALLDRLGIVLPKRMRLAEHELPAFARSA"
BASE COUNT          317 a          521 c          544 g          327 t
ORIGIN      
        1 ctactacatc atggcgtggc gggccgggaa gaccgcgctc ttgcaatcaa gcctcacccg
       61 cgcggtcgtc ttcagtgcga tgacaacggc gacggcgttc ggcagcctgt ggctgtcgaa
      121 ccatccggga acgtccagca tgggcaagct gatggcgctg gcgctggtct gcaccatggc
      181 ggccgccgtg ctgtttcagc cggccctgat gggcccaccg cgggagaagc cataacggcg
      241 cgcctgcgcc agcgacccgc gtcttgccgc ggtactctga gcacttgttt ggtcgcatga
      301 tcgggtccga aaagtctgca acttttcggg atcatgctct agcccaactt gcgcaaattc
      361 gggagtcaca cctaggcaaa tcaatggctt aaggcgagaa gtcgccacta catttcttgt
      421 gaagttggga tcaggcgctg cgtgcgaaag cgggcaactc gtgttcggcg agacgcatgc
      481 gtttcggcag gacgatgccg aggcgatcga gaagggcggc ctgggctgga tcgggttggg
      541 tgacgcaacg caagcggatc tggccatgtg tcgcggtcgg cagcacgacg tcgtgcgact
      601 gaatgcgcgc aagttcttcg agaatggtac gcggcgaatt tcccagccct gcacgctgct
      661 gccacatctc caaactcttc cacagcacga aggcaagaaa gcaaaccaag atatgcgcct
      721 gaacgcggtc ctcgcgctga tgccagatcg gccgcacctt gagctggtcc ttttggatgc
      781 ggaaagcggc ttcggcctgg gtgagttgga tataggcttt ccacagttgt tgatcgctcc
      841 aatcggcaat gttggagcgc aacagatagg cgccctctga gagcgcggcc cagtcgtcga
      901 atgccgcgtt gcaagtaacg cgaacgcgga agcctgccgg gcagctgtcc ggctccaaca
      961 caatggtgaa gcgcgcggcg gcacgctggt tctgctgcag gatgcggccg atctgccggt
     1021 tcaccactgc cggatcgagc cgtttgcgtg agcgcaccac acgagcggcg aggcgttcca
     1081 gcgctgcttc gatccgccgg ctgaacttgt cgtgcatggc cttctctttg ctgcgccggt
     1141 cggccgaacg gcacagaatc acggtctcgt cggtctcggg atggcgcgtg agtttgacct
     1201 cgacgccttc ttggaccgtg cgccagccat ccgggtgagc gaggtcgcag ccgaacttct
     1261 tcagctccga cttgggcgcc ccgatgatgt agcgtcggcc ggtctgccgc agccacgcca
     1321 gattctgagc actcgccatg ccgcgatcgg tgatccagac acgccccagc atgccatgcc
     1381 gcgcctccat ggtcgccacg atcgtctgca atgtccgcgc atcgtgggta ttgccggcaa
     1441 acacctcgta gccgagcgga aaaccgtcaa aggtgacgac aagcgcgatc aagacctgct
     1501 tgcaatcggg ccgatgatcg cgcgagtagc cgcgctgcgc ttgcggattg gcttccgcct
     1561 ggccttcgaa ataagtgctg gtgacatcgt aaagcagcac ttcgttctcg attgcgaaca
     1621 gctcaccgca gcgttgcgac agatgcgcct ccagcgcggc cttgtgggcg aggagatgat
     1681 caagcccgcg gtagagccga tccttggtg
//
LOCUS       DATLJT010001652         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1087172,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001652 DATLJT010000000
VERSION     DATLJT010001652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087172"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VM910_37580"
     CDS             <1..745
                     /locus_tag="VM910_37580"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HVI68251.1"
                     /translation="RGCQADKLLDFLRHYEADTIYLVGDIVDGWQLKSAWYWPQLHND
                     VVQKLLRQARKGVRLFYIPGNHDEFLRDYYGTHFGGIEVLEDAVHVAADGKRYLIIHG
                     DLFDVIIRHARWLALLGSNAYDLAIFINTYFNAIRRALGLTYWSLSKWAKLKVKNAMA
                     FIGEYERTLAAEARRRGGLDGVVCGHIHHAVIRDDLGLRYINCGDWVESCTAVVEHFN
                     GQFEIVRWVGPGSLPRERELAAVPLSSAI"
     gene            complement(789..1655)
                     /gene="asd"
                     /locus_tag="VM910_37585"
     CDS             complement(789..1655)
                     /gene="asd"
                     /locus_tag="VM910_37585"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259710.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HVI68252.1"
                     /translation="MTLRAHILRLLEQEDLNFLLTNRIPRRLATQFMGWFSQIEQPLV
                     RDLSIALWRFFSDLDLSEAKQPRFRSLHDCFIRELREGARPIDEDPKILVSPCDAIVG
                     ASGAIAGSSLYQIKGFPYTLADLFCDCALADAHRDGRYVTLRLTSSMYHRFHAPHDCR
                     VDQVTYISGDTWNVNPIALRRVERLFCKNERALLRTRLTATGHTITLVPVAAVLVASI
                     RLHFLDVLLGLKHRGPNVIACDAAFRKGDEMGWFQHGSTIIVFAPDGFSLHESVRENA
                     VIRVGGPLMRLP"
     gene            1691..>3144
                     /locus_tag="VM910_37590"
     CDS             1691..>3144
                     /locus_tag="VM910_37590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVI68253.1"
                     /translation="MEWASTTLAFYGLGAAALATSLVTLRRRLELSKAKHRTLTGHAR
                     MARRVAALVPFYDYSERRFFCSDDAPEEVVARRRAGFKRLSALYDTRFAETIRRTAEV
                     SDGISDLQFTDAYRVPFQYGRVVRQHLKSAAFVQSSAGVMVTDLDGNQFYDLTGSYGV
                     NVLGYDFYKQAIARAVERVGELGPVLGPYHPVIADNVQRLKALSGGDEVSFHMSGTEA
                     VMQAVRLARYHTRRSHLVRFCGAYHGWWGDVQPGIGNPLPAHETYTLKDMSEDALRVL
                     RTRRDIACVLVNPLQALHPNAAAPTDSSLVDGTRSAHFDKDAYADWLKRLRAVCTEQN
                     IVLIFDEVFVGFRLAVGGAQEYFGVRADLVTYGKTLGGGLPIGVVCGRKHLMKRFRDD
                     RPADICFARGTFNSHPYVMAAMNEFLRYLETPRARELYRELDRIWNERAQRLNQRLRE
                     EALPVEVANMSSIWTVSYLRPSRYNWMLQYYLRA"
BASE COUNT          549 a         1024 c         1010 g          561 t
ORIGIN      
        1 gcgcggctgc caggccgaca agctgctcga cttcctgcgc cattacgaag ccgacaccat
       61 ctatctcgtg ggcgacatcg tcgacggctg gcagctcaag tccgcctggt attggccgca
      121 acttcacaac gacgtcgtgc aaaagctgtt gcgccaggcg cgcaaggggg ttcgcctgtt
      181 ctacattccg ggcaatcacg acgagttcct gcgcgactac tacggtaccc actttggcgg
      241 catcgaggtg ctcgaggatg ccgtccacgt cgccgccgac ggcaaacgct acctcatcat
      301 tcacggcgac ctgttcgacg tgatcattcg gcatgcgcgt tggctcgcgc tgctgggcag
      361 caatgcctat gatctcgcga tcttcatcaa cacctacttc aacgcgatcc ggcgcgcgct
      421 cggcttgacc tattggtcac tgtcgaaatg ggccaagctg aaggtcaaga acgccatggc
      481 cttcatcggc gaatacgagc gcacgctcgc cgcggaagcg cggcggcggg gcggcctcga
      541 cggcgtcgtt tgcggtcaca tccatcacgc cgtgatccgc gacgatctcg gcttgcgcta
      601 catcaattgc ggcgactggg tcgaaagctg caccgcggtg gtcgaacatt tcaacgggca
      661 gttcgagatt gtgaggtggg tcgggcccgg ctcgttgccg cgcgagcggg agctcgcggc
      721 ggtgccgctc tcctccgcga tctaggggac aagcggaaac ggcgcctggc ggacgcccga
      781 aactgcattc acggcagacg catcagcggt ccgcccaccc gaatcacggc gttctcccgc
      841 acgctctcgt gcagcgagaa gccgtcgggg gcaaagacga tgatggtcga tccgtgctgg
      901 aaccaaccca tttcgtcgcc cttcctgaac gccgcgtcgc aggcgatgac attgggcccg
      961 cgatgcttga ggccgagcag gacatcgaga aagtgcaggc ggatgcttgc gacgagcacg
     1021 gccgcgaccg ggactagcgt aatggtgtgg ccggtggcgg tcagtctcgt ccgcaacagc
     1081 gcgcgttcgt tcttgcagaa cagcctttcg acccgccgca gcgcgatcgg attgacgttc
     1141 caggtatcgc ccgaaatgta agtcacctga tcgacgcggc aatcatgtgg ggcgtgaaag
     1201 cgatgataca tgctcgacgt cagccgcagc gtcacgtagc ggccgtcgcg atgagcatcg
     1261 gccagcgcgc agtcgcagaa caggtctgcc aacgtgtagg gaaatccctt gatctgatag
     1321 aggctgctgc cggcgatcgc gccggacgcg cccacgatcg cgtcacacgg gctgacgagg
     1381 attttggggt cctcgtcgat cgggcgcgcg ccctccctga gctcccggat gaagcaatcg
     1441 tgcaggctgc gaaaacgcgg ttgcttcgcc tcgctcaagt ccaggtcgga gaagaagcgc
     1501 cacagggcga tcgacaggtc gcgcaccagc ggctgttcga tctggctgaa ccagcccatg
     1561 aactgcgtgg ctaaccggcg gggaatccgg ttggtgagca ggaaattgag gtcctcctgc
     1621 tccagcaaac gcagaatgtg ggcgcgaagc gtcatcgtgc cgtaacctcc gtcgggtaag
     1681 gcacgattcg atggaatggg ccagcacgac tctggcgttc tatgggcttg gcgccgcggc
     1741 gctggcgacg tcgttggtga cattgcgccg gcggctcgag ctttcgaagg ccaagcaccg
     1801 gacgctgacc ggccatgcca ggatggcgcg gcgggtcgcc gcgctggtgc cgttctacga
     1861 ctacagcgag cgccggtttt tctgctccga cgatgcgccg gaggaggtcg tggcgcgccg
     1921 ccgcgccggc ttcaagcggc tctctgcgct ctacgacacg cgctttgccg agacgatccg
     1981 gcgcaccgcc gaggtgagcg acggcatctc ggatctgcaa ttcaccgacg cctaccgggt
     2041 gccgttccag tacggccgcg tcgtgcgcca gcacctcaaa tcggcggcgt tcgtgcaatc
     2101 gtccgcgggc gtcatggtca ccgatctcga cggcaatcag ttctacgatc tgaccggctc
     2161 ctacggagtc aatgtcctcg gctacgactt ctacaagcag gcgatcgcgc gcgccgtcga
     2221 acgcgtgggc gagcttggcc cggtgctcgg cccctaccat ccggtcatcg ccgacaacgt
     2281 gcagcgcctg aaagcgcttt ccggtggcga cgaagtgtcg ttccacatgt cgggcaccga
     2341 ggcggtgatg caggccgtgc ggctcgcgcg ttaccacacc cgccgatccc acctggtgcg
     2401 cttctgcggc gcctatcatg gctggtgggg cgacgtgcaa ccgggtatcg gcaacccgct
     2461 gccggcgcac gagacctaca cgctcaagga catgtcggag gacgcgctgc gcgtcctgcg
     2521 cacgcgacgc gacatcgcct gcgtgctcgt caatccgctg caggcgctgc atccgaacgc
     2581 ggccgcgccg accgactcct cgctggtcga cggcacgcgc agcgcgcatt tcgacaagga
     2641 cgcctatgcc gattggctca agcgcctgcg cgcagtctgc accgagcaaa acatcgtctt
     2701 gatcttcgat gaagtgttcg tcggatttcg cctcgccgtg ggcggggcac aggagtactt
     2761 cggcgtccgc gccgatctcg tcacctacgg caagacgctg ggcggcgggc ttcccatcgg
     2821 cgtcgtctgc gggcgcaagc acctgatgaa gcgcttccgc gacgatcgcc ccgccgacat
     2881 ctgcttcgcg cgcggcacgt tcaactccca tccctatgtc atggcggcga tgaacgagtt
     2941 cctgcgctat ctcgagacgc cgcgcgcgcg cgaactctat cgcgagcttg atcgcatctg
     3001 gaatgaaagg gcgcagcggc tcaaccagcg cctgcgcgag gaagcgttgc cggtcgaggt
     3061 cgcgaacatg tcctcgatct ggacggtctc ttacctgcgg ccgtcgcgct acaactggat
     3121 gctgcaatac tatctgcgcg ccga
//
LOCUS       DATLJT010001653         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4534895,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001653 DATLJT010000000
VERSION     DATLJT010001653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4534895"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1896..2126
                     /locus_tag="VM910_37595"
     CDS             1896..2126
                     /locus_tag="VM910_37595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68254.1"
                     /translation="MAKSPASSTLSAAELAALQALILRRPGPIFDPIDMEHVLQIDRT
                     LGKQLLAQRLETQAEVSRVVADASAKAAKLLK"
     gene            2133..3800
                     /locus_tag="VM910_37600"
     CDS             2133..3800
                     /locus_tag="VM910_37600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVI68255.1"
                     /translation="MDMSSPGERDTAAPDEPVRGCGHAEVVLIGYQDQGNLGIGYLAA
                     VLKRHGCVTETLDVREGGEKIAALLAGRQPLIVGFSLIFQFFLPQFRRVATHLREAGI
                     TSHFTIRGHYPSLCHDELLINFPEIDSVVRYEGEETLIELLDRLRAGEEWREISGIAY
                     ARGNEVVATPARPLVQDLDSLPFPQRPYEPQRIAGFPTLPLLAGRGCIRRCSFCSIHT
                     FYRTALGKVVRVRKPEKVIEEMLHLNRHHGVRVFLFQDDDFPLLGKKGRRWADELVAR
                     LHDRGLAETTVWKISCRAEYVEPELFASLREAGLFLVYMGIESGVESALDVLFKQMTV
                     EQNLDAVATLKRLDIVFAYGFMLFDPSSTFESIRGNIAFLRKIVGDGSAPAVFSRMLP
                     YGGTPIRDQLAKEGRLRGDLTHPDYNFLDLRINEYHRLLTRAVCPWIHNQGLSYSLSD
                     VADELETIERLAPGPRGGVEYRSTLRTMTAEANARLFALVEESSLAFERGDRSLLDPR
                     PVRDYCERMRARVLDLRQDFIADNLDALVENIVAHRGDGPILTPQIH"
     gene            3871..>4254
                     /locus_tag="VM910_37605"
     CDS             3871..>4254
                     /locus_tag="VM910_37605"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI68256.1"
                     /translation="MGTDPESFSGGAHCQWSSRPIPTRRVLEAVLWVLNTGAQWHMLP
                     QSYPNYKTVHRSFQAWCRDEILRRLLTDVANELRDRGALDEEECFIDATFVMAKGGGA
                     EVGATKRGKGMKIMAIVDRHGLPLSV"
BASE COUNT          810 a         1340 c         1282 g          822 t
ORIGIN      
        1 tcccggactg tcttttcagc gctctccggt atgcgcttgc tcgcttgtct catcgttgct
       61 cctcagcggg gctacgatga gccagcaatc ctctcttacg caatcagctc attctgtccg
      121 acaagtgctg acggcttaca aacctgatca agccgggcgg cttgaactcg ccctactggc
      181 tggtctttcc aaactttgac gtcaagaacc gggtcgatct caacttcaag tctgacgcac
      241 tcgcgggcac tccgcgtcgg cacgcgaagc cgcgtcgttg agatcgtgcg taaccctaat
      301 atgcgatcac gcccgattca gatcattcgc aaagcacgcg ccccctgaag acatgcggaa
      361 tgccacccgc gcactcgcag aagcatatgg atctcgcggg ttgtctccga atccttcgac
      421 aacgggcttc acagctaaga gcagcctgat gcacgatcga tagagatggg ccaagtcgac
      481 ggagagcccc gtcgtatagg gctattttgg acgacgattc ctgtatacac atgtccggcg
      541 caccgctgca ttcagcactt gcagcggtgg gaatagccaa tccgatcacc atcgctatag
      601 cgctcaacat gagtcgcatg gcgtacctcc atgataaacg cagttgtggg gttttgggcg
      661 tgctcgcttc cccttccggc gggcgaggtt gatttgccac tcgttgccac tcatggcgcg
      721 tagagcttac ataaaaagcg gcggctaaga cacccgatac ttcatgctcc ggcttcgcac
      781 gttgcggctg tgcacccatc aagggcttga cgcatctccc gccgcgacta gggatgcact
      841 ccttgccgcg cggatcggag gtctgctttc ggggcaaagc ggacgtgatg tgctcgtctt
      901 cggccgtgcg ttgcaagagg acttcgtcga gttagcggat gtgcgatctt gcatcaatcg
      961 tcggacgctg caccgcctcg gcgatcgcct ccgcgttgtt ggaggttgcc cctaaccgga
     1021 catgtggcgg acgccggagg ctcccgccta aaaacttcaa cccccgtcgg cggcctgtgt
     1081 tttccctgag ttgcgcgcgc cgctcgcccg gtcgcgtact gaccgcgagc gcgatacact
     1141 gcaacagctg acggcggatt ccgtcatcgc ccgtttgata taattggcga atctgacgcg
     1201 aatcgcttcg tacccacaaa aggacccacg acacgctaga gccggatcag ttgggacagc
     1261 gtgctctcta cgatcgtccg gaccgtctac cggggaacag agcgcgtcgc atccagcacg
     1321 atcatggacg cgctaggtgt cccagaggac caacagataa gacagaaagt cggaaaaagg
     1381 aaaccagcat cgctcgccgg acccggttat cgttgcgagc gagttcaccg aattctcgtg
     1441 tcgcagtcta tgcgtgcgcg ggtaaagtga tgccggcgca ccgtcgcttg agcctccgga
     1501 caccgaggaa aatcgaccac ttgcacatgc acgagatagg ccccgggcgg cacccggaca
     1561 cggaaattgc cgcgcctatt ggtgaccgcc gttcggcgtg gcgcaaccat aacgccgatc
     1621 tcgctgaatg tcccaaaaat acttttttgg agcactcagc agagtagcaa aaaaccgagg
     1681 ctttgacttg tgaagttgca ataaccgttg cccaaccgta tttagccaca tgttgcagcg
     1741 taccctccca gccgggttca ttccgccgtg ccttccaaca agaggtcgct gacacgcgga
     1801 tttgcccgcg ccgttttagg ttgcttcatt cctccacgca cgttaacggc gctcgcagtc
     1861 tgtcggcgga accgccactt catcgggagg tacgaatggc caagtcacct gcaagttcga
     1921 ccttgagcgc ggcagagctc gctgcattgc aggcattgat cctgcggcgg ccgggtccga
     1981 tcttcgaccc aatcgacatg gagcacgtgc tccagatcga tcgcactctc ggcaagcagc
     2041 tgctggccca acggctcgag acccaggccg aggtcagtcg cgtcgttgcg gacgcttcgg
     2101 ccaaggccgc caagctcctg aagtgaccgc cgatggatat gtcctcgccg ggcgagcgcg
     2161 ataccgccgc accggatgag ccggtgcgcg ggtgcggcca tgccgaggtg gtcctgatcg
     2221 gatatcagga ccaaggcaat ctcggcatcg ggtacctcgc ggccgtgctc aagcggcacg
     2281 gctgtgtcac ggagacgctc gacgtccgcg agggcggcga gaagatcgcg gcgctgctgg
     2341 ctggccggca gccgctgatc gtcgggttct cgctcatctt ccagttcttc ctgccgcagt
     2401 tccgccgcgt cgccacccac ttgcgcgagg ccggcatcac cagccacttc accatccgtg
     2461 gccactaccc gagcctttgc cacgacgagt tgctgatcaa ttttcccgag atcgacagcg
     2521 tcgtgcgcta cgaaggcgag gagaccctga ttgagctgct cgaccggctg cgcgccggcg
     2581 aggagtggcg cgagatcagc ggcatcgcct atgcgcgcgg caacgaggtc gtggccacgc
     2641 cggcgcgacc gctggtccag gatctcgatt cacttccgtt tccgcagcgc ccgtatgagc
     2701 cgcaacggat cgcgggtttt ccgacgctgc cgctgctcgc gggccgcggc tgcatccgcc
     2761 gctgctcgtt ctgctcgatc cacacgttct atcgcacggc gctcggcaag gtcgtgcgcg
     2821 tgcgcaagcc cgagaaagtc atcgaggaga tgctgcacct caaccggcat cacggcgtgc
     2881 gcgtcttctt gttccaggac gacgacttcc cgctgttagg aaagaaaggg cgccgctggg
     2941 ccgacgagct ggtcgcgcgg ctgcacgacc gcgggcttgc agagacgacg gtctggaaga
     3001 tcagctgccg cgccgaatat gtcgagcccg agctgttcgc gagcctgcgg gaggccgggc
     3061 tcttcctcgt ctacatgggg atcgaatccg gcgtcgagag cgccctcgac gtgctgttca
     3121 agcagatgac ggtcgagcag aacctcgacg cggtcgccac cctcaagcgg ctcgacatcg
     3181 tgttcgctta cgggttcatg ctgttcgatc cgtcgagcac gttcgagtcg atccgcggca
     3241 acatcgcgtt cctgcgcaag atcgtcggcg acggcagcgc gcccgcggta ttctcgcgca
     3301 tgctgccgta cggcggcacg cccatccgag accagctggc gaaggaaggc cgcctgcgcg
     3361 gcgatctcac ccatcccgac tacaatttcc ttgacctgcg gatcaacgag taccaccggc
     3421 tgctcacccg ggcggtgtgc ccgtggatcc acaatcaggg cctgtcgtat tcgctgagcg
     3481 acgtggcgga cgagctggag acgatcgagc ggctggcgcc cggcccgcgc ggcggcgtcg
     3541 aataccggtc cacattgcgg acgatgaccg ccgaagccaa cgcgcggctg ttcgccctgg
     3601 tggaggaatc gtccctcgcc ttcgaacgcg gcgaccggtc gctgctcgat ccgcggcccg
     3661 tgcgcgacta ttgcgaacgg atgcgagccc gcgtgctcga tctgcgccag gacttcatcg
     3721 ccgacaacct cgatgccctg gtggagaaca tcgtcgctca ccgcggcgac ggcccgatcc
     3781 tgacgccgca gattcactag aacctatctc aaaattgcgg gccgagtcga tccgctataa
     3841 atgtcggatg attcgactca cggatgagct atgggaacgg atccggaatc attttccgga
     3901 ggagcacatt gccaatggtc gtcccggccg atcccgacac gtcgcgttct tgaagcagtc
     3961 ctgtgggttc tcaacacagg tgcgcaatgg catatgctgc cgcaaagcta tcccaactac
     4021 aaaacggtac atcggagctt tcaggcttgg tgccgcgatg agattttgcg gcggctgctg
     4081 acggatgttg ctaacgagct tcgcgacaga ggcgcgctgg atgaagaaga atgcttcatc
     4141 gacgcgacgt tcgtgatggc caagggtggc ggggcagagg ttggggcaac gaaacgcgga
     4201 aaaggcatga aaatcatggc gattgttgat cgccatggat tgccgctatc ggta
//
LOCUS       DATLJT010001654         3989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2700783,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001654 DATLJT010000000
VERSION     DATLJT010001654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700783"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VM910_37610"
     CDS             complement(<1..421)
                     /locus_tag="VM910_37610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68257.1"
                     /translation="MISESDTDKYLAEILEILRERFDNGDKSALLYAIYHCLLMKRPL
                     PEWLRLAFLHTYEARARFEIRSWDEVFGRPVPKGMHLETEKRNSELRPLIIERVQKAE
                     RPIDKGLFEEIGRDLKLAGTTVSDIYYDERSRELYEMI"
     gene            complement(418..1698)
                     /locus_tag="VM910_37615"
     CDS             complement(418..1698)
                     /locus_tag="VM910_37615"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI68258.1"
                     /translation="MRQILTDALCRTKPPRNGRLEIADLRQAGLVLRITFNGARSFAY
                     RFRHPDTRKTLRATIGPYPATTLEAARKRAKDMAAQVADGTNPIEARNAEREAAPTRT
                     FQALADRYLKEHAERHKRPRSAEEDRRNLAVHVLPRWAKRDFRTVRRADVIELIEAIV
                     SAKKHTAANRVHSLISKVFSFAIDADLLEANPVARLRKRGVEKVGRRVLSDDEIRVFW
                     RGTVVSPISRPVGLALRLALLTAARASEIAGARKDEFQNLDQPEQAVWLVPSERAKNR
                     MTHLIPLAPLAVETVKNAIELSGDSEFVFPTRLSRDGPIDRHTLTVAMVRFTKSLKAP
                     TAKTWQQEIPTPHDLRRTVSTRLARMGIAKEIRDRALNHITSLRDPESRHYNLHEFQS
                     EKRDAFSRWGAELEGIIKPATVVPIRAASKGRRR"
     gene            1947..2423
                     /locus_tag="VM910_37620"
     CDS             1947..2423
                     /locus_tag="VM910_37620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI68259.1"
                     /translation="MSSPAEISMCTQQDYNQILDELHEFWGARDTRHLHHPFLVHEFG
                     NSAFVIRNGAQVVAYLFGFLSQTEPVGYVHAVAVRTSARRQGLAGELFGHFVEFARRR
                     GCSHVKAITTPANLGSIAFHKSLGMGLLGEADSDGIPVLPDYAGRGAPRVVFWKSI"
     gene            2913..>3989
                     /locus_tag="VM910_37625"
     CDS             2913..>3989
                     /locus_tag="VM910_37625"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVI68260.1"
                     /translation="MHGFRYDRILAGTALALILAIPMVGLAQGPSKMAPAPTTTSPPA
                     ASPVEEAATPAPAPAAASPAEQVSTPTLAADPAAVTEQAVAVTEQAAPADPLASLDPA
                     DRVIAEKIRDLLAATSDKIFASKKERAAAEAFYQNRNFLPLWLDKGIENARATSAVAR
                     LKHADADGLEVSDYKTPNFAGLGTDALAEAELKLTQSVLTYARHVQAGRFPYARVSRN
                     IELPQAVPEPADILGPIADAANAGKALDAFGPQHAEYQKLKAMLAEVRANSGGKKSEA
                     PRQVETTIIANMERWRWYPRDLGKAHVVVNQPDFTLRVMHEGAQVWTTRIVIGKPSMP
                     TPLLSETMKFITVNPTWHVPPSIVH"
BASE COUNT          742 a         1222 c         1220 g          805 t
ORIGIN      
        1 aaatcatctc gtaaagttcg cggctgcgct cgtcgtaata aatgtcgctg accgtggtgc
       61 ccgcaagctt aaggtcgcgg cctatctcct cgaaaagacc cttgtcgatt ggtctctctg
      121 ctttctgaac gcgctctatg atgagaggcc tcaattccga atttcgcttc tcggtctcca
      181 agtgcatgcc cttcgggact ggccggccaa atacttcatc ccatgacctg atttcgaatc
      241 gagcgcgagc ctcgtatgtg tgcaaaaagg ctagccgtag ccactccggc aatggccgct
      301 tcatcagcag gcaatgatag attgcataaa gcagcgctga tttgtcacca ttgtcgaatc
      361 tctcgcgcag gatctcaagg atttcggcga ggtatttgtc cgtgtcactt tcggaaatca
      421 tcgccgccgt cccttgctgg cggcgcgtat tgggaccacg gtggcgggtt taattattcc
      481 ctcaagctcg gcgccccagc gagagaacgc gtcccgcttt tcagattgaa attcgtggag
      541 gttgtaatgg cgactttcgg ggtcacgtag cgacgtaatg tggtttaagg ctcgatcgcg
      601 aatctccttc gcgattccca ttctggcgag cctcgtgctg acggtccgtc gcaagtcgtg
      661 cggtgtcggt atttcttgct gccatgtttt tgctgtaggg gctttcaggc tcttggtgaa
      721 gcgaaccatc gcgacggtta aagtgtgccg atcgattgga ccgtcgcggc tgagccgggt
      781 ggggaataca aactcgctgt cgccgctcag ctcgattgcg ttcttaacgg tttcgaccgc
      841 aaggggtgca agtggaatca aatgggtcat tctgtttttt gctcgctcgc ttggcacaag
      901 ccatacggct tgctcgggct ggtcgagatt ctgaaattca tccttgcgag cgccggcaat
      961 ttcgcttgcc ctagccgcag tcagtagcgc gagccgcaac gcgagcccga ccggacgaga
     1021 tattggcgac accacggtgc cgcgccaaaa cactcttatt tcatcgtcgg acaggactcg
     1081 ccgacctact ttttccacgc cgcgcttgcg tagtcgtgcg acgggatttg cttcgagcag
     1141 gtcggcatca atggcgaagg aaaagacctt gctgatgagg ctgtgaaccc ggttggctgc
     1201 ggtgtgcttt ttggcgctga caattgcctc gatgagctcg atgacatctg cgcgtcgaac
     1261 agttcgaaaa tcgcgcttgg cccatcttgg caggacatgg acggcgaggt tgcgccggtc
     1321 ttcctcggcc gagcgcgggc gcttgtgacg ctcggcatgt tctttgagat atcggtcagc
     1381 cagcgcttga aacgtgcggg taggggcggc ctcgcgctcg gcatttctgg cttcaatggg
     1441 gttggtgccg tccgccacct gggcggccat gtccttggcc cgcttgcgtg cggcttcaag
     1501 cgtcgtggcc gggtaggggc cgatggtggc gcgtaatgtc ttgcgggtgt cgggatgacg
     1561 gaagcgatag gcgaacgagc gggcaccatt gaaagtgatt cgcagaacga gtccggcttg
     1621 ccgcaggtct gcgatctcga gccggccgtt gcgcggtggc ttggtgcggc acagcgcatc
     1681 ggtcaaaatc tggcgcacgg ctgttccttt cgcagaaaaa gtctggtggt ctcacttccg
     1741 ggctggggac acaccgggga cgcaaaatcg ccgactgcaa cggaacccag ccgaacgcga
     1801 cgggacggaa aaccctcata gatctggcac tcgtaaggtg caaggccgtc cggttaaatc
     1861 cggttaagta cagctagacc agacttcgaa tctgggggtc gggggttcga atccctccga
     1921 gcgcgccatc atccagcgga gcgtcgatga gcagtcccgc agaaatttcg atgtgcaccc
     1981 agcaggacta caaccagatt ctcgatgagc tgcatgaatt ctggggcgcg cgggacaccc
     2041 gccacctgca ccatccattc ctcgttcacg agtttggaaa ctcggcattc gtcattcgca
     2101 acggagccca ggtcgtcgct tatttgtttg gctttctctc gcaaaccgaa ccggtcggat
     2161 atgtgcacgc ggtcgctgtg cggacgtccg cccgtaggca aggcctcgcg ggcgagcttt
     2221 tcggccattt cgtggaattc gctcggcggc gtggttgcag ccacgtcaag gcgataacca
     2281 cgccggcaaa tctggggtcc atcgcatttc acaaaagcct cggcatgggg ctcctcggcg
     2341 aagccgatag cgatggaatt ccggtgcttc ccgactatgc cggccgcggc gcgccacgcg
     2401 tggttttttg gaaatcgatc tagagggaca tcgtcaccaa gcatcggcgg atcggatgca
     2461 cgccgcttgg gcttcgatcg cgccccgatc cgggccggtc ccggtttacg gtcgaaatac
     2521 ctccagccgc taaccgtcac cttgggcggg caacccggtg acggcgcagg ttttcggccc
     2581 caaacgggtg gggtcgcgac ctcattaacc ttggttaacc ggcgcgacgc caagcctcct
     2641 cgctatcgcc tttgaaccaa ccgagccccc aaagcgacaa ataagtcgac agccaccgcc
     2701 ggcgagggaa ccggcactgt gggggtggtc gatcggtaac cggggagggg ggcactcatg
     2761 cacatacaag ctgggctcta acgacgagct cgccgacggc ggttcgggcc gtcgcagtgc
     2821 gcattccacg attttgcagc gtccggctca cccggcatcg cgcgcgtgcg cggcggggag
     2881 ccgtccacaa cactcttttg ggagaatctc tcatgcatgg cttccgttat gatcgtatcc
     2941 tggcaggcac ggccctggcc ctgatccttg ccatcccgat ggtcggccta gcgcagggtc
     3001 ccagcaaaat ggcacccgcg cccacgacca cttcgcctcc ggcagcttcg cctgtcgagg
     3061 aggccgcaac cccggcgccc gcgcctgccg cagcctcacc tgccgagcag gtttcaaccc
     3121 caacgcttgc cgccgatccc gccgctgtga cggagcaggc cgtcgctgtg acggagcagg
     3181 ccgccccagc cgatcctctg gcctcgctcg atcccgccga ccgtgtgatt gcggaaaaga
     3241 ttcgcgatct actggccgcg acatcggaca agatttttgc cagcaagaaa gagcgcgcgg
     3301 cagccgaggc gttctaccag aaccgcaact tcctgccgct gtggctcgac aagggcatcg
     3361 agaacgcgcg cgccacgtcc gccgtcgctc gcttgaaaca cgccgatgcc gacgggctcg
     3421 aggtcagcga ttataagacg ccgaatttcg ccggcctcgg aaccgatgcg ctggccgaag
     3481 ccgagctcaa gctcacgcag agcgtgctca cctatgcccg gcacgtgcag gccggacggt
     3541 tcccctacgc ccgcgtcagc cgcaacatcg agcttccgca ggcggtgccc gagccggctg
     3601 acattctcgg cccgattgcc gacgccgcga acgcgggcaa agcgctcgac gcgttcggtc
     3661 cgcagcacgc ggagtatcag aagctcaagg cgatgctggc ggaggtccgc gccaactcag
     3721 gcggcaagaa aagcgaggct ccgcgacagg tcgagaccac catcatcgcc aacatggagc
     3781 gctggcgttg gtatccgcgc gacctcggca aggcccacgt ggtcgtcaat cagccggact
     3841 tcacgcttcg ggtgatgcat gagggtgcgc aggtgtggac cacccgaatt gtcatcggca
     3901 agcccagcat gccgacgccg ctgctgagcg agacgatgaa attcatcacg gtcaatccca
     3961 cgtggcacgt gccgccgtcg atcgtgcat
//
LOCUS       DATLJT010001655         1831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_170851,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001655 DATLJT010000000
VERSION     DATLJT010001655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170851"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VM910_37630"
     CDS             <1..288
                     /locus_tag="VM910_37630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68261.1"
                     /translation="MTNPTLLLLDEPLEGLAPIIVEELAAAIRRMTADEGTAFILVEQ
                     HTELALSLTDDAIVLERGNIVHRARSRDLLKEQATLEHLIGLRLAEQPNAP"
     gene            complement(474..707)
                     /locus_tag="VM910_37635"
     CDS             complement(474..707)
                     /locus_tag="VM910_37635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68262.1"
                     /translation="MSAFEDAAYTMAAVTATVALFRALVRKGVLTRDEAVRVLLDEAV
                     SRAIQAEAPHEGEATNDVNRQCAEILKFIAEKL"
     gene            complement(1259..>1831)
                     /locus_tag="VM910_37640"
     CDS             complement(1259..>1831)
                     /locus_tag="VM910_37640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68263.1"
                     /translation="PIRKPEDLAGVPIAVGYQSGSHYSTIQALEQYMPADKINLTFAD
                     GMLFHRLDQLIDGKAPASALFSGPYYFAEQLGFRKVIDTTFMIATMVTGDPDPEDLRK
                     FFRALKRAQRDIDLRPDRYTHYYKKEFPTRYHAMMDTRRWGPGERLVFETYTKEVYEE
                     TFKWIADHGIFADSGMGSGNYEDAVIAAAR"
BASE COUNT          331 a          565 c          585 g          350 t
ORIGIN      
        1 atgaccaatc cgacgctgct gttgctcgac gagccgctgg aggggctcgc cccgatcatc
       61 gtcgaggagc tggccgcggc catccggcgc atgaccgcgg acgaaggcac agccttcatc
      121 ctggtcgagc agcacaccga gctcgccctg tcgttaaccg acgacgcgat cgtgctcgag
      181 cgcggcaaca tcgtgcaccg cgcgcgctcg cgagacttgc tgaaggaaca ggcgacgctc
      241 gagcacctga tcggattgcg cctggcggaa cagccgaacg cgccgtgacg tctcataccc
      301 aatccgagtc atcagctcat atactgtcgt ccccgcgtaa gcggggaccc ataatcaccg
      361 cctctggaat atgggcctcc agcataagcg cgtttacgcg cgtctttgac gcgctatgcg
      421 cggggacgac gcaatgtggt gccgagtcaa tcagcctgtc agcctgaaaa acgtcaaagc
      481 ttctccgcga tgaatttcag gatctccgcg cattggcggt tcacgtcgtt ggtcgcttcc
      541 ccctcatgcg gtgcttcggc ctggatggcc ctcgacaccg cttcatcgag gagaaccctc
      601 accgcctcgt cgcgggtgag gacgcccttt ctgaccagag cccgaaacag cgcgaccgtg
      661 gcggtgaccg ccgccatggt atacgccgcg tcttcgaatg cgctcatgtc acgctttccg
      721 gtgatggtcc aaatcgcagc gggatctcac atcgcttgcc ggggtcggat gcgtcaagac
      781 ggtggcgccg gttcgaagcg ctccggccgc tgcggaggtg agtggttgta tctcaagact
      841 agcacgaccg cgggatggtg cccgagatct gtgattgcaa tacaacactt cgccgcaaaa
      901 tcttgaccgc aagggctcat cctcttgcac cctcgccgca cggcatcaat agcgatggtg
      961 ggtcagtagc gctgctcgcg ttcctcaagc ggcgcgctgg ccgcgtgaga gcgtgtgatg
     1021 tcgtcgtatg tcatgtactg ccggacccag gccgccgagt gcgcgcggcg ggccagattg
     1081 gcgagttcgc ctgagatcgc cgccagccac cgcagtttgg ggctgcgatg gctcagactg
     1141 gcagagaaag cgcaggcggc acccttcggc cgaacgagca agccggagct cgctgcccgg
     1201 gccgcccgct cttagcgagc ttttcaacgc gccgcgactt cgaaacgcga atgatgcctt
     1261 agcgcgccgc ggcgatgacg gcgtcctcgt aattgcccga gcccatgccg ctgtcggcga
     1321 agatgccgtg gtcggcgatc catttgaagg tctcctcata gacctccttg gtgtaggtct
     1381 cgaacacgag gcgctcgccc ggaccccagc ggcgcgtgtc catcatggcg tggtaacgcg
     1441 tggggaattc cttcttgtag tagtgggtat agcgatcggg gcgcagatcg atgtcgcgtt
     1501 gcgcgcgttt gagcgcgcgg aagaacttgc gcagatcttc gggatcggga tcgccggtca
     1561 ccatggtcgc gatcatgaac gtcgtatcga tcactttgcg gaagccaagc tgttccgcga
     1621 agtagtaggg gccgctgaac agggccgagg ccggcgcctt gccatcgatg agctgatcga
     1681 ggcgatggaa cagcatgccg tcggcgaagg tcagattgat cttgtcggcc ggcatatact
     1741 gctcgagcgc ctggatggtc gaatagtggc tgcccgactg atagccgacg gcgattggaa
     1801 cgccggcgag atcctcaggc tttctgattg g
//
LOCUS       DATLJT010001656        14129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1550135,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001656 DATLJT010000000
VERSION     DATLJT010001656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550135"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VM910_37645"
     CDS             complement(<1..1339)
                     /locus_tag="VM910_37645"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVI68264.1"
                     /translation="MNAPFTARTLYGYQQRAATFFYERDAAFLVAPLGAGKGAAALTA
                     LAELIRDGHRRHALVIAPKLVAATVWPREVTLWPHLAHLRVAVLNGTPERRRELLAAA
                     PAREVTAIGVDLVPWLVGELAGLADDHPLFDVLIIDETSRLKDPSGKRARALLKAAGR
                     FRTRWGLTGTPRPNSSMDLFMPTAIVTDGTLWGRAFVPWQKRHFRPRDPFGREWAPLP
                     GAEERIAADFGTVAMTVADEDMPDLPPLNVVETHLQLPDTVMATYRRMARELFTTAEG
                     RTIEAASPMIATGKLAQIANGFLYGEGNNDVVFVHDLKIQWLKELVESLGGEPLLIAY
                     EFIEDLRTIRRALGEVPALGGATSAREALPLIAEWNEGALPLLAFHPAAAGHGLNLQY
                     GGSRMAWLSPSWSAELTEQAIARIYRPGQARHVTIHVCVAMGTVDEMKRNRVLG"
     gene            complement(1344..3554)
                     /locus_tag="VM910_37650"
     CDS             complement(1344..3554)
                     /locus_tag="VM910_37650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68265.1"
                     /translation="MFNPDDIVWFDFEAHGGALDLKAAGAFRYATAASTYANALAYAI
                     GTSPAQTWHADGKILDWDFAPDNLRAAFERGAIFAAWNATFDSCIWNFATLGFPFLAP
                     ERVIDVMVQAGVSNLPIDLESASRYLGGAGKQKDGKQLIRLFCIEGANPQQCCAEWER
                     FLAYARQDVTAMRDVYRKTRLLPQEEWQQYWAFEHINRRGVTLDMAFVRNAAALAAED
                     AVAIGVRLAVLTDGAANKVTQAKRIATWLHDNLTDAAMREILIVGVPTDDDDDGGGGD
                     GAADKSDEEEPPEFSLRRDRVARVLAMLDAKRANGGLNPDEVKAHEVATLRLYGAGAA
                     PKKFARLEAQQIDGVLRGQYRFAGAGQTGRLTSRGAQIQNLTRDVLGADGAAEGPLVE
                     AIANGCDYATLAAAGPVDVPPARKLALIVRPAIAAELGKLFAWSDWSMIEARLTPWLA
                     ASEGAERALDVFRANDRDSSLPDSYVIAAANVFHKDVRAITDWERSVGKVVILACGFS
                     GSVGALKAMALSYRIHFEDAEARRIVDAWRAANPWAEEFWGAHRDGESYGLWGAAMTA
                     IEVPGLVTRAGRLAFVFHSEYLGGTLFMVLPSGRLLTYPRPKWREVDVLDKDGNPTGE
                     KRTEMSIRRAHGRAKLWRGTLCENAVSGTAADILRITATRIETNPALAWMPIRMTTHD
                     EILCEVDAARADEAKAILRREMLTLPDWAEGLPLQSKESVGARYSKSKTALLKG"
     gene            complement(3642..4430)
                     /locus_tag="VM910_37655"
     CDS             complement(3642..4430)
                     /locus_tag="VM910_37655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68266.1"
                     /translation="MSDDHDHDRDRDRADDNGEHEESGSKQVAPVLTGSALAALNALA
                     TDLAKVNTAAIIGRSGKPMLLFKREGSGTWMFGQKKTIPEPDSHWAVNPLTFQWGYIC
                     FGDNNKKLGEHLVSVTQPKPLITDLPDLGFKWQEQWAVEMKCIDGADAGIEVIFKTNT
                     DGGVKALVILFDQVRDQLNRDLAKPPKERDGKIVAITKLGKESYVHKQYGTTWKPVLD
                     AIDWMPLSGPAPTPKEPEPTPPQSSGPGPNTAGAAEQPRRRRVA"
     gene            complement(4427..5683)
                     /locus_tag="VM910_37660"
     CDS             complement(4427..5683)
                     /locus_tag="VM910_37660"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HVI68267.1"
                     /translation="MSPQAHSPFGGSVAARVLRCPGSVDLISKVPPELHKSSDYADRG
                     TSMHMAMAALIAEDVHVPVESLAGKKFGDYTITHDDIENFLRPAYSFVEKLLDVPGAA
                     YYLDQRVIFPAIANTFGTLDLLIRIDHTVHVIDFKFGVGVRVLALTPDGDDDVLNSQL
                     LFYGAAARHSLPEFFTGIEDIVLTILQPVTTDADSEMVSTVTVTHAELDEFIAAYRVA
                     CAEALSEAPRLKAGPWCRFCSARPICKEHTKPLLDLAAFTVPAPWSTTRAFFGTPTAK
                     EEYLKVLAAGLELADAVKDTCTALHDQAKRALENGDNVPGYALSNGRAERYWRDEITA
                     QVALLSLGFEHDDLVEVEVMRSVKQIELRAKARGLKIPPELIISRRSGVSLKRSENVR
                     VPVLGRDELARAFSEALAVFTEEARQ"
     gene            complement(5680..6174)
                     /locus_tag="VM910_37665"
     CDS             complement(5680..6174)
                     /locus_tag="VM910_37665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68268.1"
                     /translation="MKILGIDPGINGAVAVIEMMDGVDGIAPKFLGVTDIPIVGAAAK
                     TRVDAIELRNWIETHNPDFAGIERAGSMPGQGVASSFKFGRATGTIEAVVACCGVPMV
                     LIEPSKWKRAFHLNADKEASRQLAIQLFPHAHGELNLKRWHNRAEAMLIALFTANNRT
                     GRAL"
     gene            complement(6171..6779)
                     /locus_tag="VM910_37670"
     CDS             complement(6171..6779)
                     /locus_tag="VM910_37670"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HVI68269.1"
                     /translation="MPAPISAPLATTNDTSQSDDETMRLALRAAVKAAGSQRALARMI
                     GVTHPAVALWNRAPAKHVINIERATGIPREQLRPDLYSQLTLPEAIKILKAAGYRVSK
                     PRPPKKFKRGKDRVGPTFVAEFADGAVTRMSTFTSVEKLDWDRGVRLSIAAYQSRWRL
                     ARAQYREWNGKRYLLHLPAAPVPPTIIAMQFEQDGKVLEVRP"
     gene            complement(6832..7428)
                     /locus_tag="VM910_37675"
     CDS             complement(6832..7428)
                     /locus_tag="VM910_37675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68270.1"
                     /translation="MTAAETTSGARPKNAHIFAKAEGLHYVEPEWVSSRLFEVEQFPG
                     LIWEPSAGWGRIAEAARAAGYTVRATDITDRGYPLDAVEDFLNIDHIDPKTSIVGNPP
                     FTDAIVQHAIKLNPIKMALVWPFARIVAAWPWLATAPLAHIWMLTPRPAMPPASYIAA
                     GKKPEGARVEHCWLIFERDYDGLPQLGWMHRDRNGHYA"
     gene            complement(7521..8165)
                     /locus_tag="VM910_37680"
     CDS             complement(7521..8165)
                     /locus_tag="VM910_37680"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68271.1"
                     /translation="MKTVFDYAAILATLQQVAPEAHIAGGAVRDTILQKEIADIDVFM
                     SDEHVEAAAALLRSSCSYVKVGEWKQYLGFSDPAMTRVAKFEKADEPIPVCIIGLKPE
                     FVNPHANIARFDFGICMVAFDGHEFIRTADFDQDAEEQTFTLYRADNEAQFVYSMSRF
                     EKLTANRYQGWTLAIPKEFEGLAKEHTFRKHWYHEFGKYFEGKNLLRPKERIAA"
     gene            complement(8235..8921)
                     /locus_tag="VM910_37685"
     CDS             complement(8235..8921)
                     /locus_tag="VM910_37685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68272.1"
                     /translation="MQNALLNIATSGGGQQPFVGDTSTALSNFDLKHLNQRDRAELVV
                     VDATIRAELNAVGHASTTALVHGFALGEALIALKKLLGRGFWMREGEAWVNAKYGLSA
                     RSARDYMRLASHREALEDQIGSSAANLSVRGALRLIGPAKTARKRVKPSGLKTANWRA
                     ATTDQRAAFVSAIPLTEWLEVIPADWRAEIGDRSNKHVTSLERQVAGLKKQVAESVPL
                     RLVKTTKKAA"
     gene            complement(9066..9359)
                     /locus_tag="VM910_37690"
     CDS             complement(9066..9359)
                     /locus_tag="VM910_37690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68273.1"
                     /translation="MAQFKIAERKRRSRVAACAQTLPTPPPFPTFPSGSLLAMTIPEF
                     ARLHGMSVAMFFKMQSQGEGPEMMAIGRRRMVSMESAARWRAAREKAATEAAE"
     gene            complement(9430..9903)
                     /locus_tag="VM910_37695"
     CDS             complement(9430..9903)
                     /locus_tag="VM910_37695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68274.1"
                     /translation="MQPRFGWRLRLVAAFDDKKHRWSAEIVSAVGEAWERPVYEDIWT
                     QEVVEGIEVYGEAVYRDVLRTITVKPPYQWEFDVDELVTLLPIGAGDKGGNWETHVIQ
                     EIEDLGRKTVSDLHKSKQLRPHLEAVLKRVNKRIPNDPKVLRTLILAFLRGKLNK"
     gene            10392..11936
                     /locus_tag="VM910_37700"
     CDS             10392..11936
                     /locus_tag="VM910_37700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68275.1"
                     /translation="MTRNISGGTGNGNGDNNDNIIHLPATEAERRQMRKLLRAQERQR
                     LVDVFIGEDGRQLFHDPNGVAYADPIIGGHRETWLVKSKEFRSALIGHLRRLHDRLVS
                     EQSILAFEIKAAMTKRAVNAQIDDFETRAIGSTSAVRTVCVRVAEHEGSIYIDLCNPN
                     WEAIRVNAGGWYIIADPPVRFRRTRGMLELPHPERGGKLEMLRPFLNVKTDADFIMCV
                     AWTLAAMRAHGPYTILNLYGEHGSAKTHLLQFLRSLFDPHTTATTRLPFGSRDLYIAA
                     RNSHAQMFANVSSISDAMSDDLCRLATGEGMRTRELFTDSGEALFGGERPIAIEGINR
                     SVIKPDLLSRSAVLEIPPLSGYLTAHALRARFEAQRAKIFGALLDTMVRGLAALPTVQ
                     LINPPRMADFAEWGVACGLPDFEAVYAANRREAISVMLDHDLLARALREFMTRRKQWR
                     GIAEELLTAIGPAAKVGSTQSLSDQLRRLAPALRTVGLNVVHEPRKNQRRPLLIEKVT
                     PVTIPL"
     gene            complement(12552..13757)
                     /locus_tag="VM910_37705"
     CDS             complement(12552..13757)
                     /locus_tag="VM910_37705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68276.1"
                     /translation="MSNGRPTMRNYLDRMHDQTLAHVASLSNSDILSKSLDEWVAQLV
                     QEFASPEIPVLDLTKISRTAEQGEVPAYAVPNLKFSTHVPRVQGLIHHIHVPFTGDRN
                     FFHCHPAGWVDEFPGAAVGADELILAIGGAWYTPPQIEDKIDSQIADIENALQGFRDE
                     AERFKVNFPDMVRPALEKRRQTAEAEAQTAAGLKYPIKPRPNAPQTYVVPAVRKKIAP
                     APMPAGPSPDPTLLEEHYRNILNIMDNMARVMERSPSAFEKIGEEDLRWHFLLQLNAQ
                     YDGVNGEAFNFQGKTDILVRQQNHNLFIAECKYWSGQKGLLETVDQLFKYVTWRDTKT
                     AVVMFSDRQQFTPVVKDAADTMAQHPRLVGKPKKEGETRFRYTLHIPNDKDRHITTTM
                     MLFNVPKPT"
     gene            complement(13917..>14129)
                     /locus_tag="VM910_37710"
     CDS             complement(13917..>14129)
                     /locus_tag="VM910_37710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68277.1"
                     /translation="SRAYPDIGGLMSYGASLVDAYRHIGVYTGRILKGAKPADLPVVQ
                     RGNLCEKFPPNWHFFRRVQKMRDIAG"
BASE COUNT         2543 a         4458 c         4391 g         2737 t
ORIGIN      
        1 tgccgagcac gcggttgcgc ttcatctcat cgacggtgcc catcgcgacg cagacgtgga
       61 tggtcacgtg ccgcgcctgc ccgggtcggt agatgcgcgc gatcgcctgc tcggtcagct
      121 cggccgacca gctcggcgac aaccacgcca tgcgcgatcc gccatattgc aggttgagcc
      181 cgtggccggc cgcggccggg tgaaaggcga gcagcggcag cgccccttca ttccactctg
      241 cgataagagg caacgcttcg cgcgccgacg tggcgccgcc gagcgccggc acctcgccca
      301 gcgcgcgccg aatggtccgc aggtcttcga tgaactcgta ggcgatgagc agcggctcgc
      361 cgccgaggct ttcgaccaac tctttcagcc actggatctt aaggtcgtga acgaagacca
      421 catcgttgtt gccctcgccg tagaggaagc cattcgcgat ctgcgccagc ttgccggtcg
      481 cgatcatcgg cgaggccgct tcgatcgtgc gcccctcggc ggttgtgaac agctctcgcg
      541 ccatcctccg gtaggtcgcc atcacggtgt ccggcagctg caggtgcgtc tcgacgacgt
      601 tgagcggggg caggtcgggc atatcctcgt cggcgaccgt catcgccacg gtgccgaaat
      661 cggcggcaat ccgctcctct gctcccggca gcggcgccca ttcgcgcccg aacggatcgc
      721 gcggccggaa gtgccgcttc tgccacggca cgaaggcgcg accccacagc gtgccgtcgg
      781 tcacgatggc ggtcggcatg aacaggtcca tgctcgagtt cggccgcggc gtgccggtca
      841 ggccccatcg cgtccggaat cgcccggcgg ccttgaggag cgcgcgggcg cgcttgccgc
      901 tcggatcctt gagccgcgac gtctcgtcga tgatcagcac gtcgaacagc gggtgatcgt
      961 cggcaaggcc ggcgagctcg cccaccaacc agggcacgag atcgacgccg atcgccgtca
     1021 cctcgcgcgc cggtgcggca gcgagcagct cccgccggcg ctcgggcgtg ccgttgagaa
     1081 cggcaacgcg cagatgcgcg agatgcggcc agagcgtgac ctcccgcggc cacacggtcg
     1141 cggcgacgag cttgggggcg atgacaagcg cgtggcggcg gtgcccgtcg cggatcagct
     1201 cggcgagtgc cgtgagcgcg gccgcgccct tgccggcgcc gagcggcgcc accaggaacg
     1261 cggcgtcgcg ttcatagaag aacgtcgcgg cacgctgctg gtagccgtac agggtgcgcg
     1321 cggtgaaggg cgcgttcata cttctacccc ttcaaaagcg cggttttcga cttgctgtag
     1381 cgggcgccca cgctttcctt gctttgcagc ggcaagcctt cggcccagtc cggcagcgtc
     1441 agcatctcgc gccgcagaat tgccttcgcc tcatcggcgc gcgccgcgtc gacctcgcac
     1501 aggatctcgt cgtgcgtggt catgcggatt ggcatccatg cgagcgctgg gttggtctcg
     1561 atccgcgtgg ccgttatgcg caggatgtcc gcggccgtgc cgctaacggc attctcgcag
     1621 agcgtgcccc gccacagttt cgcgcgcccg tgtgcgcgcc ggatcgacat ctcggtgcgc
     1681 ttctcaccgg tcggattgcc gtccttgtcc aggacgtcga cttcgcgcca ctttgggcgg
     1741 gggtaggtca gcaggcggcc cgatggcagc accatgaaca gcgtgccgcc gaggtattcg
     1801 gagtgaaaga cgaacgcgag gcggccggcc ctggtgacga gaccgggcac ctctatggcg
     1861 gtcatcgcgg cgccccagag gccgtagctc tcgccgtcgc ggtgcgcgcc ccagaactcc
     1921 tcggcccacg ggttagctgc gcgccaggcg tcgacgatgc gacgcgcttc cgcatcttcg
     1981 aagtggatgc ggtaggagag cgccatagcc tttagtgcgc cgacgctgcc tgaaaaacca
     2041 caagcgagga tcaccacctt gccgacacta cgctcccagt ccgttatcgc acgcacgtcc
     2101 ttgtggaaga cgttggccgc ggcgatgacg taactgtcgg gcagcgatga atcgcggtcg
     2161 ttagcgcgga agacgtcgag ggcacgctcg gcgccctccg aggcagcgag ccagggcgtg
     2221 agccgcgcct cgatcatcga ccaatccgac cacgcgaata gctttcccag ctcggcggcg
     2281 atggccggac gcacgatcaa cgccagcttg cgcgccggcg gcacgtcgac gggaccggcc
     2341 gcagccagcg ttgcgtagtc gcagccattg gcgatcgcct cgaccagcgg accctccgcg
     2401 gcgccgtccg caccgagcac gtctctcgtc aaattttgga tttgcgcccc gcggctggtc
     2461 aggcggccgg tctgcccggc gccggcgaac cgatactgac cacgcagcac gccgtcgatc
     2521 tgctgcgctt caaggcgtgc gaacttcttc ggcgcggcac cggcgccata gagccgcagc
     2581 gttgcgactt cgtgcgcttt gacctcatct ggattgagac cgccattggc gcgcttggca
     2641 tccaacatcg ccagtacgcg ggcaacgcga tcacgcctca gactgaattc tggtggctct
     2701 tcttcgtcgc tcttatcagc ggcgccatcg ccaccgccgc cgtcgtcgtc atcatcggtg
     2761 ggaacgccga cgataagaat ttcgcgcatc gccgcatcgg tgaggttgtc gtgcagccaa
     2821 gttgcgatcc gttttgcctg cgtgaccttg ttggcagctc catcggttag cacggccaac
     2881 cgaacgccaa tggcgacggc atcctcggcc gcgagcgccg cggcattgcg cacgaaggcc
     2941 atgtcgagcg tcacaccgcg gcggttgatg tgctcgaacg cccaatactg ctgccattcc
     3001 tcctgcggca gcagccgcgt cttacgatac acgtcgcgca tcgctgtaac gtcttgacgc
     3061 gcataggcga gaaagcgctc ccactccgcg cagcactgct gcgggttcgc gccttcgata
     3121 caaaacagtc gaatgagctg cttgccgtct ttttgcttgc cggcgccacc gaggtagcgc
     3181 gacgcacttt cgagatcgat cggcaggttg gagacgccgg cttgcaccat cacatcgatg
     3241 acgcgttctg gcgcaaggaa gggaaatcct aaagtggcga aattccaaat gcagctatcg
     3301 aaggtcgcgt tccaggccgc gaagatcgcg ccacgctcga aggcggcgcg caggttgtct
     3361 ggcgcgaaat cccagtcaag gatcttgcca tcggcgtgcc aggtctgcgc tggtgaggta
     3421 ccgatcgcgt aggccagagc gttggcgtac gttgatgctg cggtggcata gcggaacgcg
     3481 ccggcggcct tcaggtcgag cgcgccgccg tgggcctcga aatcgaacca gacgatgtcg
     3541 tcggggttaa acatcggaat ttccgagaac tcagaaaagt caggcgaggc cgccgctcgg
     3601 ccggcctcgc cttgatcggg tgcgcgcgta cgcgcaccgg cttaggcaac gcgtcgacgt
     3661 cgcggctgct cggcagctcc ggccgtatta gggcccggac ctgacgactg cggcggtgtc
     3721 ggctccggct ccttaggagt cggtgctgga ccgctcagcg gcatccaatc gatagcgtcc
     3781 agcacaggct tccaagtcgt cccatactgt ttgtgcacgt agctctcttt tccgagcttc
     3841 gtgatcgcta cgatcttgcc gtcacgctct ttcggaggct tggccagatc gcggttgagc
     3901 tgatcgcgta cctggtcaaa caagatgacg agggctttaa cgccaccatc cgtgtttgtt
     3961 ttaaagatca cttcgatgcc agcatctgcg ccgtcgatac atttcatctc gacggcccat
     4021 tgctcctgcc acttgaagcc tagatcgggc aggtctgtga tcagcggttt cggttgagtg
     4081 acagaaacaa gatgctcacc gagcttcttg ttgttgtcgc cgaaacagat atacccccac
     4141 tggaacgtca acggattgac ggcccagtgg ctgtctggct cggggatggt cttcttctgc
     4201 ccgaacatcc aggtgccgct gccttcgcgc ttgaacaaca acatcggctt gccgctacga
     4261 ccgatgatgg cggccgtatt gaccttggcg aggtcagttg cgagcgcatt cagggcagca
     4321 agcgcgctgc ccgtgagcac gggggcgact tgcttgctcc cggactcttc gtgttcgccg
     4381 ttatcgtcgg cgcgatcgcg atcgcgatcg tggtcatggt cgtcactcat tgtcttgcct
     4441 cctctgtgaa gactgcgagt gcctcggaga atgcgcgtgc aagctcgtcc cgtccgagga
     4501 ccgggacgcg cacgttctca ctccgcttca atgagacacc ggagcggcgt gaaatgatta
     4561 gttcgggtgg gattttgagg ccgcgcgctt tcgctcggag ttcgatctgt ttgaccgaac
     4621 gcatcacctc aacttctaca agatcgtcat gctcgaagcc gaggctcagc agagctacct
     4681 gtgcagtgat ctcatcacgc caatagcgct cggcgcggcc gttcgaaagc gcatagcctg
     4741 gtacgttatc gccgttctcc agcgcgcgct tagcctgatc atgcagcgcc gtgcaggtgt
     4801 ccttgaccgc gtcggcaagc tcgagtcctg ccgcgaggac tttcagatat tcttctttcg
     4861 ctgttggtgt gccgaagaac gcgcgagtcg tgctccacgg cgctggcacc gtaaaggcag
     4921 cgagatcaag aagcggtttc gtgtgctcct tgcagatcgg tcgcgctgaa cagaacctgc
     4981 accatggccc cgccttcagc cgtggtgctt ccgacaacgc ttccgcgcaa gcgacgcgat
     5041 aagctgcgat gaattcgtcc agctcggcat gcgtgaccgt aacggtcgag accatctcgc
     5101 tatcggcatc ggtcgttacg ggttggagga tcgtgaggac gatgtcctcg atcccggtga
     5161 agaattcagg cagcgagtgg cgcgccgcgg cgccgtaaaa cagcaactgt gaattgagaa
     5221 catcgtcgtc gccgtctgga gtgagcgcga gaacgcgcac gccgacaccg aatttgaaat
     5281 cgatcacatg aaccgtgtgg tcgatgcgga taagcaaatc gagcgtgccg aaggtgttgg
     5341 cgatcgcagg aaagatgaca cgttgatcga ggtagtaggc ggccccaggc acgtctaaga
     5401 gcttttccac gaagctgtat gccggccgca ggaagttctc gatatcgtca tgcgtgatcg
     5461 tgtagtcgcc gaacttttta cccgcgagac tctcgactgg gacgtgcacg tcctcggcga
     5521 tgagagcggc catcgccatg tgcatgctcg tgccgcgatc agcataatcg gatgacttgt
     5581 gcagctctgg aggcactttc gagatgagat cgactgaccc cgggcagcgc agaacccgcg
     5641 ccgcgacact gccgccgaac ggtgaatgcg cctgcgggct catagcgcac gccccgttcg
     5701 gttgttggcg gtgaacagag cgatcagcat cgcttcagcg cggttgtgcc agcgcttgag
     5761 gttcagctcg ccatgggcgt gcggaaacaa ttggatcgcg agttgcctcg aagcttcctt
     5821 gtcagcgttg aggtgaaacg cgcgcttcca tttcgaaggc tcgatcagca ccatcgggac
     5881 gccacagcag gcgactacgg cctcgatcgt tccggtcgcc cggccgaact tgaacgacga
     5941 cgcgacgccc tgccccggca tcgatccggc acgctcaatg ccggcaaagt cgggattatg
     6001 ggtttcgatc cagttacgca gctcgatggc gtcgacccgc gtcttcgccg cggcgccgac
     6061 gatcggaata tcagtgacgc cgaggaactt cggcgcgatg ccgtcgacgc cgtccatcat
     6121 ctcaatgact gcgaccgcgc cgttgatgcc gggatcgatg ccgaggattt tcacggccgc
     6181 acctccagca ccttgccgtc ctgctcgaac tgcatcgcga tgatggtcgg aggaactggt
     6241 gcggcaggaa gatgaagcag atagcgcttg ccattccact cgcggtattg agcgcgtgcg
     6301 agccgccagc gcgattgata cgccgctatc gacaagcgca cgccgcggtc ccaatcgagt
     6361 ttctcgaccg acgtgaacgt gctcatgcga gtgacagcgc cgtcggcaaa ttcggcgacg
     6421 aaggtcggac cgacgcggtc cttgccgcgc ttaaacttct tcggcgggcg cggcttgctg
     6481 acgcgataac cggccgcctt aagaattttg atggcttccg ggagtgtaag ttgagagtag
     6541 agatcaggcc gcagctgctc acgcggtatg ccggttgccc gctcgatgtt gatgacatgc
     6601 ttggcgggag cgcggttcca tagagcaacg gctggatgag tgacgccgat catgcgtgca
     6661 agcgcgcgct ggctgcctgc tgccttcact gctgcgcgca atgccagccg catcgtctcg
     6721 tcgtcggatt gcgaggtgtc gttcgtggtc gccaacggcg cgctgatagg agcgggcatg
     6781 gcgcccgctt cattcgtctt gatgagcgca agcagtggcg gggtgtccat cttacgcata
     6841 gtgtccgtta cgatcgcggt gcatccatcc gagttgcggc agcccgtcgt aatcgcgctc
     6901 gaagatgagc cagcaatgct cgactcgagc gccctcaggc ttcttgccgg cggcgatgta
     6961 ggacgcgggc ggcatcgccg gccgcggcgt cagcatccag atgtgagcga gcggtgcggt
     7021 cgcgagccat ggccacgccg cgacgatccg cgcgaagggc cagacgagcg ccatcttgat
     7081 cggattgagc ttgatcgcgt gctgcacgat cgcgtcggtg aagggcggat tgccgacgat
     7141 cgatgtcttg ggatcgatgt ggtcgatgtt gagaaagtct tcgacggcgt cgagcggata
     7201 cccccgatca gtgatgtcgg ttgcgcgcac cgtgtagccg gcggcgcgag cggcctcggc
     7261 gatccgtccc cagccggcgg acggctccca gatcagacca gggaattgct cgacctcgaa
     7321 cagccgactc gagacccact cgggttctac gtagtgcagg ccctctgcct tcgcgaagat
     7381 gtgcgcgttc ttcgggcggg cgccggaggt agtctctgcg gcggtcatca cacaaaccct
     7441 cgaagaaaaa ttgccaaaca ggaaatcgcg aactgagaaa aataggccgc gcatttacgc
     7501 gcggccgagg cgacgagagt tcatgccgct attcgctcct tcggccgcaa aaggttcttg
     7561 ccttcaaagt acttgccgaa ttcgtggtac cagtgcttgc ggaacgtgtg ctctttcgcg
     7621 agcccttcga attccttagg gatcgccagc gtccagccct ggtagcggtt agcagtgagt
     7681 ttctcgaaac gagacatcga gtagacgaac tgcgcctcgt tatcggcgcg gtacagcgtg
     7741 aaagtctgtt cctccgcgtc ctggtcgaag tccgcagtcc gtatgaactc atgaccgtcg
     7801 aacgcgacca tgcatatgcc gaaatcaaac cgtgcgatgt tggcatgcgg attgacgaac
     7861 tcgggcttca acccgatgat gcagaccggg atcggctcgt ccgccttctc gaatttagcg
     7921 acgcgcgtca tggccgggtc ggagaagccg aggtactgct tccactcgcc gaccttcacg
     7981 taactgcatg atgaccgcag cagcgcggcg gccgcctcga cgtgctcgtc gctcataaac
     8041 acgtcgatgt cggcgatttc cttctgcaag atcgtgtcac gcacggcacc gccggcgatg
     8101 tgcgcctccg gcgcgacctg ctggagcgtg gcgaggatcg ccgcgtagtc gaatactgtc
     8161 ttcatcaacg tctcctcttt gttatcgcgt agaaaaatag gaccgccgac gttgccgccg
     8221 acggcttgcg ttgttcatgc cgccttcttc gtcgtcttga cgagtctcag cgggaccgac
     8281 tcggcgacct gcttcttgag tccggcgacc tgcctctcaa gagaagtaac gtgcttattg
     8341 gagcggtcgc cgatctcagc ccgccagtcc gccgggatga cttccagcca ttcggtgagc
     8401 ggaatggcgc tgacgaacgc tgcacgctga tccgtcgtcg ccgccctcca gttcgcggtc
     8461 ttcaagccgg aaggcttcac ccgcttgcgc gccgtcttag cggggccgat caaccgcaag
     8521 gcgcccctga cgctcaaatt ggcagcactg ctgccaattt ggtcttcaag agcctcgcga
     8581 tgactcgcaa gccgcatgta atcgcgtgcg ctgcgagcgc ttaggccgta cttggcattg
     8641 acccaggcct ccccctcgcg catccaaaaa ccgcgcccaa gaagcttctt gagtgcaatc
     8701 agggcctcgc cgagagcaaa tccgtggacg agcgccgtcg tcgaagcgtg accgacagcg
     8761 ttcaactcgg ctctaatggt ggcgtccacg accacaagct cggcgcggtc tctctgattg
     8821 agatgcttga ggtcgaagtt ggaaagagcg gttgaagtgt cgccgacaaa aggctgttgc
     8881 cccccgccgc tcgttgcgat attgagaagc gcgttctgca tggaacgtag ctccgtcttt
     8941 ggcgaccggg ggctcttgcg aagaagtcac cccggcccgc cgccctccac accgttacgc
     9001 ttcaactcac gccccgccgc cgtgtggact cgagatcacg tcgagacgaa ttcgatctgt
     9061 tccgcctact ccgcggcttc cgtggctgcc ttctccctcg ccgcccgcca gcgcgccgcg
     9121 ctctccatcg agaccatgcg tcgccggccg atcgccatca tttctggacc ttcgccttgc
     9181 gactgcattt tgaaaaacat cgcgaccgac atcccgtgca accgggcaaa ttccgggatc
     9241 gtcatcgcga gaagagaacc cgacggaaaa gtcggaaaag gcggaggggt gggtaaagtc
     9301 tgagcgcaag ccgctacgcg gctgcggcgc ttacgctcgg cgattttgaa ttgagccacg
     9361 ttcggtctcc acgcagtttc atcgtggaga cctccaataa tcccgtggat gtggggtata
     9421 aaagggaaat tacttgttaa gtttcccccg caggaaagcc aagattaatg tgcgcaaaac
     9481 cttcgggtcg tttgggatgc gcttgttgac ccgcttcaaa acagcttcta gatgcggacg
     9541 taattgttta gatttatgta agtctgacac ggttttacga cctaagtcct ctatttcttg
     9601 aatgacgtga gtttcccaat tccccccttt gtcccccgcg cctatcggaa gcaatgtcac
     9661 cagctcatcg acgtcgaatt cccattgata tggcggcttc accgtgatcg tcctgaggac
     9721 atcccggtag acggcctccc cgtagacttc gatgccttcg acgacttctt gggtccagat
     9781 gtcctcgtag actggacgct cccacgcttc gccaaccgcg ctcacgatct cggccgacca
     9841 gcgatgtttc ttatcgtcaa atgccgccac gagccttaac cgccagccaa aacgcggctg
     9901 cactgcgacg tcatcgcaaa aaagtaaacc tggccgccgg cggaacgcct ccgtgatccg
     9961 atcagcggca tccgtcggtg agtattctga ccgcaagatg atagtgacag cctcaagcgg
    10021 cgagcatcgg ctagccatgc ctggcgtact acaccaagct ctatcgtgac gtccaccttg
    10081 tccaccagac agcggaaatg ctgttgattt atcggtcgga gtcaattacc acttgatcat
    10141 cgcgcgttag gtatctcgca cccgccagag cgcccaaaga ccaccgccag cgcccctcgg
    10201 gcccgcatcg gtcggcgcca agtcgaagtc gagccgcgat ttcccgaaac gattgcgtgc
    10261 agacgccacc gaaaggtgcg cttgcaacga ctcgatgggc tcaatcgagt caccgaagaa
    10321 gcgtgagcag caaagggttg cccggaccgt cgctacgaac agcggccggg caaaggacga
    10381 aggagtttgg gatgacccgc aacataagcg gcggcactgg caatggcaac ggcgacaaca
    10441 acgacaacat cattcacctc cctgcgactg aggcggagcg gcgccagatg cgcaagctct
    10501 tgcgggcgca ggagcggcag cggctcgtcg atgtcttcat cggcgaggat ggccgccagc
    10561 tctttcacga ccccaacggg gttgcctacg ccgacccgat catcggaggc caccgcgaaa
    10621 cgtggctggt caagtcgaaa gagtttcgca gcgcgctcat cggccacttg aggcgtctgc
    10681 acgatcgcct ggtcagcgag cagtcgatac tagcattcga gataaaggcc gccatgacca
    10741 agcgcgcagt caacgcgcag atcgacgact tcgagaccag ggccatcggc agtacgtcgg
    10801 ccgtccgcac tgtctgcgtg cgcgtcgccg agcacgaggg cagcatctac atcgacttat
    10861 gcaatccgaa ttgggaggcc atccgcgtca acgccggcgg ctggtacatc atcgccgatc
    10921 cgcccgtgcg gttccggcga acgcgcggca tgttggaact gccgcacccc gagcgcggtg
    10981 gcaagctcga aatgctgcgg ccgttcttga acgtgaaaac ggacgcggac ttcatcatgt
    11041 gcgtggcgtg gacgctcgcg gctatgcgtg cgcacggacc gtacacaatc ctcaaccttt
    11101 atggcgagca cggtagcgcc aagacgcatc tgctccagtt tctgcgcagc ctgttcgacc
    11161 cgcacacgac tgcgactacc cggctcccgt tcggcagccg cgatctatac atcgccgccc
    11221 gtaacagcca cgcccagatg ttcgcgaacg tttcgtcgat ttccgacgcg atgtcggatg
    11281 acttatgtcg gctggcgacc ggcgagggca tgcgcacccg cgaattgttc acggactcgg
    11341 gtgaggcgct attcggcggc gagcgcccga tcgcaatcga aggtatcaac cgctccgtca
    11401 tcaagcccga cctcctgagt cgctcagccg tcctagagat accgccattg tccggatacc
    11461 tgacggcaca cgcgctgcgg gcgcggttcg aggcgcagcg agcgaaaatt tttggtgcgc
    11521 tgctcgatac gatggtgcgc gggctcgcgg cgctgcccac ggtccagctt atcaacccgc
    11581 cgcgcatggc ggacttcgcg gaatggggcg tcgcttgcgg actgccggac ttcgaggcgg
    11641 tctatgcggc caaccgccga gaagccatca gcgtcatgct cgaccatgat ctgctcgcgc
    11701 gggcgctgcg agagttcatg acccgacgga aacagtggcg cggcatcgcc gaggaactgc
    11761 ttaccgcgat cggcccggcg gcgaaggtcg gcagcacgca gtcgctgtcg gaccagctac
    11821 gacgcttggc accagcgctg cggacggttg gcctcaacgt cgtgcacgag ccgcgtaaga
    11881 accagcgtcg gccgctgctg atcgagaagg tgacgccggt gacgatacca ctgtgagggg
    11941 tcgtcaccgg cgtcacgcta ttcagtgcga cacgcatgga gaggtcgtca ccggcgtcac
    12001 cgcggtcgcc cggagcggcg ggtcatgggg gtagaagtat cgcggtcgcg ggggtctagg
    12061 gggtagaagt atttttagcg ccgcgccggc cgcgcgcgcc gctcggccgg ccgcgcgtcg
    12121 cgagctcagc cgacacgccg gcgccctgat ggcgggcctc ctcggctggc tgcggcccgc
    12181 gctccacggc gcttttttgc tgccgcggac actctacggg caccggggcc ggaccgactc
    12241 gagaaaccct tgcggcatcg gcataacccg cgccaagcgc cgaattaggc caggcccttg
    12301 tagggctttt gcactctcca cgcagctcca ccgtgctcca ccgaacttcc ggaatcgctt
    12361 gcgttcgccg agagctcgac ttggtatcat cagagctgct ggaagagcat gagtcgctga
    12421 gaggaaggct ggtcgcccga gttcgcgttt cgttagtgac agtgcttgac gccggcctcc
    12481 acccccagcc ggcgacattc caccatcgcg tccgaatacc aggcagcgac ctctaagcgg
    12541 cggggtcctg actaggtcgg cttggggaca ttgaacagca tcatggtggt cgtgatgtgc
    12601 cggtccttgt cgttcggaat gtgtagcgtg tagcggaagc gcgtctcgcc ttccttcttc
    12661 ggcttgccga cgaggcgggg gtgctgcgcc attgtatccg cagcatcctt cacgacaggt
    12721 gtgaattgct ggcgatccga gaacatcacc acggcggtct tcgtgtctcg ccaagtcacg
    12781 tacttgaaga gctggtcgac cgtttcgagt aagccctttt gccctgacca gtacttgcat
    12841 tccgcgatga acagattgtg gttctgctga cgcaccaaga tgtcggtctt gccctgaaag
    12901 ttgaacgctt ccccgttcac tccatcatat tgagcgttga gctgcagcag gaagtgccag
    12961 cgcaggtctt cctccccaat cttctcgaag gcgctggggc tgcgctccat cacccttgcc
    13021 atgttgtcca tgatgttcag aatgttccgg tagtgctcct ccagcagcgt gggatccggg
    13081 ctcggtccgg ctggcatagg cgcgggcgcg atcttcttcc gaacagccgg gactacgtag
    13141 gtctggggtg cattcgggcg gggcttgatt gggtacttca ggcctgctgc tgtctgggcc
    13201 tcggcctcgg cggtctggcg ccgcttttcc agagcaggcc taaccatgtc ggggaagtta
    13261 accttgaacc gctcagcctc gtcccggaag ccttgcaggg cgttctcaat gtcggcgatc
    13321 tggctgtcga ttttgtcctc gatctgcgga ggcgtgtacc acgccccgcc gatagcaagg
    13381 atcagttcat cggcacccac tgcagcgcct gggaactcat cgacccagcc ggcgggatgg
    13441 cagtggaaga agttgcggtc gcccgtaaac ggcacgtgga tgtggtggat cagcccttgc
    13501 accctcggca catgagtgga gaattttagg ttcggcacgg cataggcggg cacctcgcct
    13561 tgctcggcgg tgcgactgat cttcgtcagg tcgagcaccg ggatttctgg gctggcgaac
    13621 tcctgcacta gctgtgcgac ccactcgtcc agcgatttcg acagaatgtc ggagttggac
    13681 aggctggcca catgcgccag cgtctggtcg tgcatccgat cgagataatt cctcatcgta
    13741 gggcggccat tcgacatctc aattatcccg gtggtgcccg cgacaattcc tcgggccggt
    13801 gacatagtgc gcgtcgccgc cttcgggcgg ctggtggacc gtgcgctcag cgcctgccca
    13861 tgaacggccc cggttctgga ggcaggtcgg gccgttcgca tgttcgggtc cgcgattcat
    13921 ccagcgatat cccgcatttt ttgcacacgg cgaaagaagt gccaatttgg cgggaatttc
    13981 tcgcacaagt tgccacgctg cacgaccggc aaatccgcag gcttcgcgcc cttgaggatg
    14041 cgcccggtgt acacgccgat gtggcgatac gcatccacca ggctcgctcc gtagctcatc
    14101 agcccgccga tgtcgggata ggcacgcga
//
LOCUS       DATLJT010001657         3232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1322288,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001657 DATLJT010000000
VERSION     DATLJT010001657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322288"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VM910_37715"
     CDS             <1..896
                     /locus_tag="VM910_37715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI68278.1"
                     /translation="MRSLNLDQLRAFVEVVARGSFTAAAKELNLTQPAVTHQVQELER
                     RFRVTLVERFGKRAYLTQAGEKLIVHARQLLEDDARAQMEMRKLNDGWLGRVRVGTSA
                     TVLMYALPPILRRLKTAHPQLEISLKTLLTATTLEMLKTNALDLGLCALPVEDPAFEV
                     TSLFEDELVAILPAEMTKIPKKVTPAFLSRCPLILVNEGSALRRTVGEWLAHAGPPPK
                     PLMEFDNVEPIKSVVAVGLGCSVVPRMCLGGGHVATARTLVRPLSPSVGRQVGVIRLR
                     GKRSTEGIDLAWAALLTLQRR"
     gene            complement(1261..1635)
                     /locus_tag="VM910_37720"
     CDS             complement(1261..1635)
                     /locus_tag="VM910_37720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68279.1"
                     /translation="MQFDELLRKLAGRHQSLDPPRVPVLIVLDARKQIGLAENADQLA
                     VGIDAFRQVTAAIAGGCVICAGLREEGLLVGFFPAPIAESKKDQRRAPAPEHPPMPTR
                     PPPRAGRRRARSTCNRRTWSDP"
     gene            1748..2056
                     /locus_tag="VM910_37725"
     CDS             1748..2056
                     /locus_tag="VM910_37725"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVI68280.1"
                     /translation="MSQNGKVVGIVSRSKLIQALASSKPAADSRRESDRTIRLELLNR
                     LGQQQWTDFGSRNVIVEDGVVHLWGLVGSESERKALIALAQEVPGVSDVADEMIPAYW
                     "
     gene            2181..2516
                     /locus_tag="VM910_37730"
     CDS             2181..2516
                     /locus_tag="VM910_37730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68281.1"
                     /translation="MVELQPNAVGDGFADNHWHRRYPRAEPRRQMIDKARVGAALGKK
                     AAAGAQALANNPRGCSALSAASTLAFRGVIHTKVNAVVLFYFIDKKIFQNVLPACWGE
                     RFFADSAMN"
     gene            complement(2771..>3232)
                     /locus_tag="VM910_37735"
     CDS             complement(2771..>3232)
                     /locus_tag="VM910_37735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68282.1"
                     /translation="TGVEVEWVGTDVDLGAMLERGEIDALISADAPKAILEGSPKVGR
                     LFEDYEAVERDYYRRTGIFPIMHTVVVTRELADEHPNLVKAVYRAFCTAKDRMQEQYV
                     KGMTFNNMSIMLPWFSNLIDEDRALLGDDWWPYGIQRYHALVLPPCLGAQT"
BASE COUNT          592 a          949 c         1066 g          625 t
ORIGIN      
        1 ccatgcgcag tctcaatctc gatcagctga gggcgttcgt cgaggtcgtc gcgcgtggca
       61 gtttcaccgc cgcggcgaag gagctgaacc tgacgcagcc cgcggtgacg catcaggtgc
      121 aggagctcga gcggcgtttc cgtgtcacct tggtggagcg cttcggcaag cgcgcctacc
      181 taacgcaagc gggtgagaag ttgatcgtcc atgcgcggca gcttctggaa gacgatgcgc
      241 gcgcgcagat ggagatgcgc aagctcaacg acggctggct cggccgggtc cgcgtcggta
      301 cgagcgcgac cgttctcatg tatgcgctgc cgccaatcct gcgaaggctc aagaccgccc
      361 atccgcaact cgaaatcagc ctcaaaacac ttttgaccgc gacgaccttg gaaatgctca
      421 agacgaacgc gctcgacctg gggctgtgtg ccctacccgt cgaggatccg gcgttcgagg
      481 tgacctcgct gttcgaggat gagctcgtcg ccattctgcc cgccgagatg acgaagatac
      541 cgaaaaaggt gacgccggca tttctttccc gctgcccgct gatcctcgtc aacgaggggt
      601 cggcgcttcg ccgcacggtg ggggagtggc tggcacatgc cggacccccg ccgaaaccgt
      661 tgatggagtt cgataacgtc gagccgataa agagcgtcgt ggcggtgggg ctcggttgtt
      721 cggtggtccc taggatgtgt ttgggcggcg gacacgttgc gacggcgcgt acgctcgtgc
      781 ggccgctcag cccgtccgtg ggccggcagg tcggagtcat tcggttgcgc ggaaagcgca
      841 gcaccgaggg catcgatctg gcatgggcgg ctctgttgac actgcagcgc cggtgaccaa
      901 cgcgcgcatg cgccagcggc acgggatgcc gcgcgcgtga tcgcgcgccg tgggcggaca
      961 cagagcgtgc cggctgccag gaggccaacg aagcgctcgg aaggccaagc tgatcgacta
     1021 ggccgcctca gttggcggcc tcatttgcag tcgtggcttt tggcagattg tgttgcaaaa
     1081 gtccaaaact gtccggtgat aaattttccg ctatcaccgc cgccctggtc gcggcgctga
     1141 tcacgccggc ggcggcccag tcacgtcttg tggttctgga tcggccgacg cttgcctgct
     1201 ctgaccagca ggcactggag acggcgctca cacttgcgcg gcttcacgat gaccccgcgt
     1261 tcagggatca ctccaggtcc tacggttgca tgtggatcga gcccgacgac ggcccgctcg
     1321 gggtggtggg cgggtcggca tggggggatg ttcaggtgct ggtgcccggc gctggtcttt
     1381 tttggattcg gcgataggcg cagggaagaa cccgaccagt aagccttctt cccggagccc
     1441 cgcgcaaatc acgcaaccgc cagcgatcgc cgcggtgacc tgacgaaagg cgtcgatgcc
     1501 gacggcgagc tggtcggcat tctcagcgag gccgatctgc ttgcgcgcgt cgaggacgat
     1561 gagcaccggc acccgcggtg ggtcgaggct ctgatgccgg ccggcaagct tgcgcaagag
     1621 ttcgtcaaac tgcacggcaa gaaggtttct gaggtgatgt cgaccaatgt cgtctcggcg
     1681 agcgaggaca cgccggtgtc cgaaatcgct gcgtcattgg agcgccatcg cattaagcgg
     1741 gtgccgattg tcgcaaaacg gcaaggtcgt cggcatcgtc agccgctcaa agctcatcca
     1801 agcccttgcc tcgtccaaac cggcggccga ctccagacgg gaatccgacc gcaccatacg
     1861 cctcgagctg ctcaatcgat tagggcagca gcagtggacg gactttggca gccgcaacgt
     1921 catcgtggag gatggcgtcg ttcatctatg gggcctcgtg ggatcggaat ctgagcgcaa
     1981 ggctttgatt gcgctggcgc aagaggttcc gggggtatct gacgtggccg acgagatgat
     2041 ccccgcatat tggtagcgcg ggaacgttgc cttcggctga tcgaacgttg ttcgcggccc
     2101 gcttggaggc tggagtcccg ccgggatttc gcgcgggcct ttcgcgggcg ggttctcggg
     2161 tgcgtccgtg gggcgcggtc ttggtcgagt tgcagccaaa cgcggtgggc gacgggtttg
     2221 cagacaacca ttggcatcgg cgctatccgc gtgcagaacc tcgtcggcag atgatagaca
     2281 aggcacgggt tggggccgcc ctcggtaaga aggcggcagc gggggcgcaa gcgttggcca
     2341 acaacccgcg aggctgctcg gcgctaagcg cggcttcgac gctcgccttt cggggggtca
     2401 ttcatacgaa ggttaatgcc gttgtgcttt tttactttat cgacaagaaa atatttcaga
     2461 acgtactccc cgcctgctgg ggcgaacggt tctttgcaga ctctgcaatg aattaggcgg
     2521 ctcggaagtt tggggtcggc acgcacacga acaagcttgt agcgtgatgt gcatcgtggg
     2581 cacataaaat cggcagcttc agtcatgacg gaccttagtt aagcaccaat aaacgaattc
     2641 gaccactgac catgctagcc tgccggtcgc tgcagcgcaa accgcaacga ttagagaaac
     2701 cgggatcact tgaggtatgg ccatgctact ggggtatggg tggccttccg tcgggccgct
     2761 ttcgtgccgc tcacgtctgc gctccgagac aggggggcag aacaagggcg tgatatcgct
     2821 ggatgccata gggccaccag tcgtcaccca gaagcgcgcg atcctcgtcg atcaggttgc
     2881 tgaaccaggg cagcatgatg ctcatgttgt tgaacgtcat tcccttcaca tactgctctt
     2941 gcatgcggtc cttggcagtg cagaaggcgc ggtagaccgc cttcacgaga ttaggatgct
     3001 cgtccgcaag ttcgcgggtc accaccacgg tgtgcatgat cgggaaaatg ccggtgcgtc
     3061 ggtagtaatc gcgctccacg gcttcataat cctcgaacag ccttccgacc ttcggcgagc
     3121 cttcgagaat tgccttaggg gcatcggctg agatgagcgc gtcgatctcg cctcgttcca
     3181 gcatggcgcc aagatcgaca tcagtgccga cccattcgac ttcgacgccg gt
//
LOCUS       DATLJT010001658         4870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3388445,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001658 DATLJT010000000
VERSION     DATLJT010001658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3388445"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /gene="htpG"
                     /locus_tag="VM910_37740"
     CDS             <1..1615
                     /gene="htpG"
                     /locus_tag="VM910_37740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HVI68283.1"
                     /translation="GIGMDRQELIDNLGTIARSGTKDFVAKLAEAKDGASLIGQFGVG
                     FYSAFMAADRIVVTSRRAGASEVWTWSSSGSSGFEVAPADEETAGRVARGTEIVLHLK
                     QEAAKYLEAHEIERIVGAYSDNIQFPIELVPEEGESRQINSASALWQRSKSELTPEDY
                     KQAYKSIANAFDEPAMTLHYRAEGRYSYAVLLFAPSTKPFDLFEPARKGKVKLYVRRV
                     FITDDADLLPAYLRFIRGVIDSEDLPLNISREMLQNNPQLVQIRKAVTGRVIGELETL
                     SEKEPDAFNKIWDAFGPVIKEGIYEDFERREKLLALSRFSTTAGEKRSLKQYVADFKP
                     NQTEIYFLAGDSIERLKSNPRLEAAAARGIEVLLLTDPVDAFWTSMRLDYDGKPLKSL
                     SQGDISFDLIPLVEEHKDEAEPAADEAATIAIIKASLGDRVSDVKASTRLTTSASCLV
                     AGSHGPDRELERILSQQARGARTRPILEINLRHPLVAAIAKKGGDAKVADDLSLLLLE
                     QAQILDGELPEDPAAFAARLNRLVLQGVA"
     gene            complement(1666..4809)
                     /locus_tag="VM910_37745"
     CDS             complement(1666..4809)
                     /locus_tag="VM910_37745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVI68284.1"
                     /translation="MNESISSLFIRYPIGTSLLMAGILFVGLVAYPLLPVAPLPQVDF
                     PTIQVSASLPGGSPETMAASVAQPLERQLAQIPGIAQMTSTSSLGSTAVTIQFDLNRN
                     IDGAANDVQGAINAASGQLPKSLPSPPTYRKVNPADSPILLLSASSDTLPLIKVSDAV
                     DAQLGQQISQISGVAQVFIGGQQKPAIRIQVDPAKLVAKGLSLEDVRSQVAISTVDSP
                     KGNIDGEKRAFTIYANDQLFEAHDWNNVIIAYRNGGPLRIRDIGQAIAGPEDMKTAAW
                     ADGKRGVFLVIFKQPGANVIDTVDRIKAQLPRLIAAIPPAIKIKIMSDRTLTIRAAVE
                     DVQITLLITIVLVIMVIFIFLRSFWATVIPAVTVPLALLGACALMWAFGYTLDNLSLM
                     ALTIAVGFVVDDAIVMLENITRYVEQGERPLAAAFKGSSEIGFTIVSISVSLVAVLIP
                     LLLMGGVIGRLFREFAVTLAMAIFVSMIVSLTLTPMMASRFLRAHSETRHGRFYQWSE
                     RAFERMLHAYERGLDLALRWSKTTLAIFFATIALSVYLFVIIPKGFFPQQDNGFLTGV
                     SEMSQDISFSDMKRKQEELSRIVQADPAVDSIAMFIGGGGTALNSGRMYITLKPHDER
                     NADAQQIIARLRPKLEKVEGARLYMQASQDVRLGGRATRTQFEFTLQDANLTELNEWA
                     PKILARMQKLPELRDVATDQQTEGTTLELKIDRDAAARYGIEPQLIDDTLYDAFGQRQ
                     VAQYFTQLNSYHVVLEILPEMQGRVDTLDKIYVKSPTTGDEVPLSVFCKWTTVPVRPL
                     AIAHQGQFPAVTISFNLGEGVALGQATSAVQKAMDTMGAPATLSSSFQGTAQAFQQSL
                     GTVPLLILAALVVVYLILGVLYESYIHPLTILSTLPSAGVGALAILMAFGFDFSLIAL
                     IGIILLIGIVKKNGIMMVDFAIVAEREEHLSPVESIRKAAILRFRPIMMTTMAALLGG
                     VPLMLGTGTGSEIRQPLGYAMVGGLLVSQALTLFTTPVVYLYLDTASNAISRWTRGRT
                     PPSEPPAEGEDIKHAAE"
BASE COUNT          946 a         1472 c         1574 g          878 t
ORIGIN      
        1 cggcatcggc atggaccggc aggagctgat cgacaatctc ggcaccatcg cccgttccgg
       61 caccaaagat tttgtggcga agcttgcgga agccaaggat ggcgccagcc tgatcggcca
      121 gttcggtgtc ggtttctatt cagccttcat ggcggcggat cgcattgtcg tcaccagccg
      181 ccgcgcgggc gcgagcgagg tctggacctg gtcgtcatcc ggcagttcgg gcttcgaggt
      241 cgcgccggct gatgaggaga cggccgggcg cgttgcgcgc ggcaccgaga tcgtgctgca
      301 tctgaagcag gaagctgcca aatatctgga agcccacgag atcgagcgca tcgtcggcgc
      361 ttattccgac aacatccagt tcccgatcga actcgttccg gaagaaggcg agtcgcggca
      421 gatcaattcg gcgagcgcgt tgtggcagcg ttcgaagtca gagcttacgc ctgaggatta
      481 caagcaggcc tacaagtcga tcgccaacgc cttcgacgag ccggcgatga ccctgcatta
      541 ccgcgccgaa ggccgttatt cctacgcggt gctgttgttc gcgccttcga caaagccgtt
      601 cgacctgttc gagccggcgc gcaagggtaa ggtcaaactc tatgtgcgac gcgtcttcat
      661 caccgatgac gccgacctgc tgccggctta cttgcgcttc atccgcggcg tgatcgacag
      721 cgaggacttg ccgctcaata tctcccgcga gatgctgcaa aacaatccgc agcttgtgca
      781 gatccgcaaa gctgtcaccg gccgcgtcat cggcgagttg gaaacattga gcgagaagga
      841 gccggacgcg ttcaacaaga tttgggatgc cttcggcccc gtgatcaagg aggggatcta
      901 tgaagatttc gagcgacgcg agaaattgct ggcgctgtcg cggttttcaa cgaccgccgg
      961 cgaaaagcgt tcgctgaagc aatatgtcgc ggacttcaag ccgaaccaga ccgaaatcta
     1021 ctttctggcg ggcgactcca tcgagcggtt gaaatccaat ccgcgactgg aggcggcagc
     1081 cgcgcgcggc atcgaagttc tgctgctgac cgatcccgtc gacgcgttct ggacgtcgat
     1141 gcggctcgat tatgacggca agccgttgaa gtcgttaagt caaggcgaca tcagtttcga
     1201 tctcatcccg ctcgtcgaag agcacaagga cgaggcggag cccgcggccg acgaagcggc
     1261 gacgattgcg atcatcaaag cgagcctcgg cgatcgcgtc agcgacgtga aagcgtcgac
     1321 gcggctgacc accagcgcat cgtgcctggt cgccggcagc catggacccg atcgcgagct
     1381 cgagcgcatc ctgtcgcaac aggcgcgcgg tgcgcgaacc aggccgatcc tcgagatcaa
     1441 tttgcggcat ccgctggtgg cggcgattgc gaagaagggc ggcgacgcca aggtcgccga
     1501 tgacctgtcg ctgctgttgc tggagcaggc gcagatcctc gacggcgagc tgccggagga
     1561 tccggcggcc ttcgcggcac ggctcaaccg tctggtgctg caaggcgtgg cataggagcc
     1621 ccgagggcct cggctgcgag catcgcgccg aacccgccgg cgcggctact cggccgcgtg
     1681 cttgatgtct tcaccctccg ccggcggttc ggaaggtggc gttctgccgc gggtccagcg
     1741 cgagatcgcg ttggaggcag tgtcgagata gagatagacg accggcgtcg tgaacaatgt
     1801 cagcgcctgg ctgacgagaa ggccgccgac catcgcatag ccgagcggct ggcggatctc
     1861 cgagcccgtg ccggtcccga gcattagggg cacgccgccg aggagggctg ccatcgtcgt
     1921 catcatgatc gggcgaaacc gcagaatcgc agccttccgg atcgattcca caggcgaaag
     1981 gtgctcctcg cgttcggcca cgatcgcgaa gtcgaccatc atgatgccgt tcttcttcac
     2041 gattccgatc aggagaatga tgccgatcag ggcgatcagg ctgaaatcga acccgaacgc
     2101 catcaggatc gcgagtgcgc cgacgccggc ggacggcagg gtcgagagaa ttgtgagcgg
     2161 atggatgtag ctctcataga gcacaccgag gatcaggtag acgacgacca gcgccgccag
     2221 gatgagcagc ggcaccgtgc cgagcgattg ctggaacgcc tgcgccgtgc cctggaagct
     2281 ggaactcagg gtcgccgggg cacccatggt gtccatcgcc ttctggacgg cgctggttgc
     2341 ctgtcccagc gccaccccct caccgagatt gaagctgatg gtgaccgccg ggaactggcc
     2401 ctggtgcgcg attgcgagcg ggcgcaccgg caccgtcgtc catttgcaga atacggagag
     2461 cggcacctcg tcgcccgtgg tcggcgattt gacatagatc ttgtcgagcg tatcgaccct
     2521 gccctgcatc tcgggcagga tctccagcac cacgtgatag ctgttgagtt gggtgaaata
     2581 ctgcgcgacc tggcgctggc caaacgcgtc gtagagcgtg tcgtcgatca gctgcggctc
     2641 gatgccgtaa cgtgccgcgg cgtcgcgatc gatcttgagc tcgagcgtgg tgccctcggt
     2701 ctgctggtcg gtcgcgacgt cgcgcagctc gggcagcttc tgcattcggg ccagaatctt
     2761 cggcgcccat tcgttgagct cggtgagatt ggcgtcctgc agcgtgaact cgaactgagt
     2821 tcgcgtggcg cggcctccga gtcgcacgtc ctgggaggct tgcatgtaaa gccgggcgcc
     2881 ctcgaccttc tcgagcttcg gacgcaaccg cgcgatgatc tgctgggcgt ccgcgttgcg
     2941 ttcgtcgtgc ggcttcagcg tgatgtacat gcgcccggaa ttgagcgccg ttccgccgcc
     3001 gccgatgaac atcgcgatgc tgtcgacggc tggatcagcc tgcacgattc ggctgagctc
     3061 ctcctgcttg cgcttcatat cggaaaacga gatgtcctgc gacatctcgg aaaccccggt
     3121 cagaaagccg ttatcctgct gcgggaagaa gcccttcgga atgatcacga acagatagac
     3181 cgacagcgca atggtcgcga agaagatcgc aagcgtggtc ttgctccagc gcagcgcgag
     3241 atcgaggccg cgttcgtagg cgtgcaacat tcgttcgaac gcgcgttcgc tccactggta
     3301 gaaccgcccg tgcctggttt cgctgtgggc gcgcagaaag cgcgaggcca tcatcggggt
     3361 cagcgtcagc gacacgatca tggatacgaa aatcgccatc gcgagcgtca ccgcgaattc
     3421 gcggaacaga cggccgatca cgccgcccat cagcagcaga ggaatcagca ccgcaaccag
     3481 cgagacgctg atcgagacga tcgtgaagcc gatctcgctg gagcccttga atgcggcggc
     3541 gagcggccgt tcgccctgct cgacataacg ggtgatgttc tccagcatga cgatggcatc
     3601 gtcgaccacg aagcccacgg cgatcgtcag tgccatcagc gacagattgt ccagcgtata
     3661 gccgaacgcc cacatcagcg cgcaggcgcc taacagcgct agaggcacgg tgacggccgg
     3721 aatgaccgtt gcccagaagc tgcgcagaaa aatgaagatc accatgatca ccagcacgat
     3781 ggtgatcaaa agcgtgatct gcacgtcctc gaccgcggcg cggatcgtca gcgtgcgatc
     3841 gctcatgatc ttgatcttga tcgcgggcgg aatggctgcg atcaatcgcg gaagctgcgc
     3901 cttgatgcga tcgacagtat cgatcacgtt ggcgccgggc tgcttgaaga tgacgaggaa
     3961 aactccgcgc tttccgtcgg cccacgcggc cgtcttcatg tcttcgggac ccgcgatcgc
     4021 ctgtccgatg tcgcggatgc gcaacggacc gccgttgcga taggcgatga tgacgttgtt
     4081 ccagtcgtgg gcctcgaaga gctggtcatt cgcgtagatc gtaaaagcgc gcttctcgcc
     4141 gtcgatattg cccttgggac tgtcgaccgt ggaaatcgcg acctggctgc ggacatcctc
     4201 gagcgaaaga cctttggcga cgagcttggc gggatcgacc tggatcctga tcgcaggctt
     4261 ctgctggccg ccgatgaaca cctgcgcgac acccgagatc tggctgatct gctgcccgag
     4321 ctgagcatca acggcgtcgc tgaccttgat caggggcaat gtgtccgagc tcgccgacag
     4381 caagaggatc ggcgagtccg ccggattgac tttgcgatag gtcggcggcg aaggcaggct
     4441 tttcggcaat tgtccgctgg cggcattgat cgcgccctgc acgtcgttcg cggcaccatc
     4501 gatgttgcgg ttgagatcga actggatcgt gaccgccgtc gagcccagcg aactcgtcga
     4561 cgtcatctgc gcaatgcccg ggatctgcgc aagctgccgc tcgagcggct gcgccacgga
     4621 ggccgccatg gtttcagggc tgccgccggg gaggctggcg gaaacctgga tggtcgggaa
     4681 gtcgacctga gggaggggag cgaccggcag cagcggatac gcgacaagac cgacgaacaa
     4741 aatcccggcc atcaacagcg atgtcccgat cgggtatcgg ataaatagcg aggagatgct
     4801 ttcgttcatg cccagatcga aacggctggc gcatcttttc ccgaaaaccg ggagccgcac
     4861 tcgaaagccg
//
LOCUS       DATLJT010001659         4737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4536399,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001659 DATLJT010000000
VERSION     DATLJT010001659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4536399"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1573)
                     /locus_tag="VM910_37750"
     CDS             complement(242..1573)
                     /locus_tag="VM910_37750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI68285.1"
                     /translation="MSEVASFVAAHSGAKSDVEMSTIRTISWRLIPFLVLAYFFSYLD
                     RVNLGFAALTMNADLKFSPLVFAWGAGIFFIGYVLFEVPSNLALEKFGASRWIARIIV
                     TWGIVSMLMVTVSGEVSFYVLRFLLGVAEAGFFPGIILYLTYWYPAEYRARFLAAFGI
                     AVPISTVIGAPISGLLLGLDGVAGLKGWQWLFIIEGIPAVLLGIVSWFFLTDRPEHAN
                     WLKPEQKAWLAAKLNSEIAAKEAVKRVTLGEALSSPKVITLALIYFGFVAALYGMQFW
                     LPQIVKAFGLSNAQTGFVTAIPYLFGTIAMIFWARHSDSSRERVFHVGAPFFLTAIAL
                     AAASYLTDPTATMVALTLAAIGIFAIFAVFWTLPTAWLSGTAAAGAIALINSIGNLAG
                     FGGPYLVGWVKEATGSTSSGLLVLALLPLIGGLLVLFGDHESNAEFAGGAR"
     gene            complement(1817..2986)
                     /locus_tag="VM910_37755"
     CDS             complement(1817..2986)
                     /locus_tag="VM910_37755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HVI68286.1"
                     /translation="MSPAPKPFPLSKREARRIWLAAQRLDLPVPFGAGPQAVADAVAH
                     LGYVQIDTINVIERCHHHILWNRIPDYRRDDLRQAQSVDRSVFEYWTHALSYVPTRDF
                     RYFIPEMRLHRREGHRWFASVKPADTRKVMRLLRQGALTIRDIEDDVLTEKEHLWSSR
                     KPSKRALQLAFYSGRVTISERNGMLKTYELIERHFGWDKLPKGASAGDFTAYLLERGL
                     RSQGLVSLDSICHLDAPRKTAVRRLIERRVRRGELVAVALEGTGKQEHWASPAVLEAS
                     RETGPELVHILSPFDPLIIQRKRTHLFFDYEHRFEAYVPKAKRKFGYFALPVLAGDEI
                     VAAIDLKTDRQKKKLLLQKWSWVGHGKAARGEARKDLKRKIEEELHRFERFQLGD"
     gene            complement(3122..3430)
                     /locus_tag="VM910_37760"
     CDS             complement(3122..3430)
                     /locus_tag="VM910_37760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68287.1"
                     /translation="MITSAKRHIEYQGQRSAAERTIIISTMPVLIGAAMVAAAILLAT
                     LVNAIGTRYVGLEGPSEESAWLIDRLTGHVYRCEASQRGKANCDADIATGSIPERPKP
                     "
     gene            complement(3714..>4737)
                     /locus_tag="VM910_37765"
     CDS             complement(3714..>4737)
                     /locus_tag="VM910_37765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVI68288.1"
                     /translation="GAAINVDVTLEQTMADAKVKLVFLDACRDNPFAAKIRSAKATRS
                     VSVQSGLAEMKSGEGTLIAFATGPGQTALDGENGTNSPFTRALVANITQPGVEIQQAM
                     TKVRAQVNEETNKNQLPWGHTNLIGSVYLNPLGVPSEKAEAPSTPPGPASDVELEFWR
                     SVKDSNKPEELNAYLTSYPNGTFKSLALARIASLQNGPSTTTRNLTTEVDPATFTEEA
                     DQTSEDQIGLDKGQRRDVQRRLTGLGFDVKVTGKFDDQTRSVIRRWQAARGYPATGYL
                     NNLQHKALLSEIVSARVASTDSSSEGSGHVRHSGGGGGGRRYYRGGGGGAPGGFFGFM
                     GGLFRH"
BASE COUNT          940 a         1427 c         1504 g          866 t
ORIGIN      
        1 gtgtccgggg gccggacatt aattaacgtt cacgaaatcc tgattattga cgattattga
       61 cttttgtcag tgataactcg atacttccta tcagatccaa gaacgggagt atccgatgtt
      121 ggctcggtcg attttatcga attatcaatg atgaaagtca atattcgtca ataatcagga
      181 tttcgtgaac gttaattaat gtccggcccg gacacacgcc cggtcgcgcc cgggcgaaaa
      241 cctaccttgc cccgcccgca aactccgcgt tgctctcgtg gtcgccgaag aggacgagca
      301 agccgccgat caggggcaac agcgccagca ccagaaggcc ggacgaggtc gagccggtcg
      361 cttccttgac ccagccgacc agataggggc cgccgaagcc cgcgagattt ccgatcgaat
      421 tgatcaacgc gatcgcgccg gcggctgccg tgcccgacag ccacgcagtc ggcagggtcc
      481 agaacaccgc gaagatggcg aagatgccga tcgcagccag cgtcagcgcc accatggtcg
      541 ccgtgggatc ggtcagatag ctcgcggcgg cgagcgcgat ggccgtcagg aagaacggcg
      601 cgccgacatg aaagacgcgc tcgcgggacg agtcggaatg tcgcgcccag aaaatcatcg
      661 cgatcgtgcc gaacagataa ggaatcgcgg tgacgaaccc ggtctgtgca ttgctgaggc
      721 cgaaggcctt gacgatctgc ggcagccaga actgcatgcc ataaagcgca gcgacgaagc
      781 cgaaatagat cagtgcgagc gtgatcacct tgggcgagga cagtgcctcg ccgagcgtga
      841 cgcgcttgac cgcctctttc gccgcgatct cggagttgag ctttgcggca agccacgcct
      901 tctgctccgg cttgagccag ttcgcgtgct cgggcctgtc ggtgaggaag aaccaactga
      961 cgatgccgag cagcaccgca ggaatgccct cgatgatgaa gagccattgc cagcccttca
     1021 gacccgcaac accgtcgagc ccgagcaaca gccccgagat cggcgcgccg atcacggtcg
     1081 aaatcggaac ggcgatcccg aacgcggcca ggaagcgcgc gcgatattcg gccggatacc
     1141 aataggtgag atagaggatg atgccgggaa agaagccggc ctccgccacg ccgagcagaa
     1201 agcgcagcac gtagaagctc acttcgccgc ttaccgtcac catcagcatc gagacgatgc
     1261 cccaggtcac tatgatgcgg gcgatccagc ggctcgcgcc gaacttttcc agcgccaggt
     1321 tgctcggcac ctcgaagagg acatagccga tgaagaagat ccccgcgccc caggcaaaga
     1381 ccaggggcga gaatttcaga tcggcgttca tggtcagcgc ggcgaagccg agattgacgc
     1441 gatcgaggta ggagaagaag taggccagca ccaggaacgg aatcagccgc caggagatgg
     1501 tgcggatggt ggacatctca acatcgcttt tggcgccgct atgcgctgcg acaaaactgg
     1561 cgacctcgct catttacgtt tctcccctgc ccgctcttgt tgatggattt tcgggtccag
     1621 ccgttttgag catcggcgca ggcaagtcaa cgggatttgc aaaaaagcgg ggaatagcgc
     1681 gttgcgccga gagaaattgg gatgacgagc gcgcgagccg ctgcgagtgc cgaagaaaat
     1741 ctctgcggag cgaaaggata gcccaacacg aacgcaagcg aaccatgagc tgtgtttggg
     1801 ctaactggac tcgccgtcaa tcgccgagtt gaaaccgctc gaagcggtgc agctcttcct
     1861 cgatcttgcg cttgagatcc ttgcgcgcct cgcccctggc agccttgccg tgaccgaccc
     1921 agctccattt ctgcagcagc agttttttct tttgccggtc ggtcttcagg tcgatcgccg
     1981 cgacgatctc gtcaccggcc agcaccggca gcgcgaaata accgaacttg cgtttcgcct
     2041 tcggcacata ggcctcgaaa cgatgctcgt aatcgaagaa cagatgcgtc cgcttgcgct
     2101 ggatgatgag aggatcgaac ggcgagagga tatgcacgag ctcgggccct gtctcgcggc
     2161 tcgcctccag caccgcgggc gaggcccaat gctcctgctt gcccgtgcct tcgagcgcga
     2221 ccgcgacgag ttcgccgcgc cgcacgcgcc gttcgatcaa ccgccgcacc gccgtcttgc
     2281 gcggcgcgtc gaggtggcag atcgaatcga gcgagaccag gccttgcgac cgtaaccccc
     2341 gctcgagcag ataggcggtg aagtcgccgg cgcttgcgcc cttcggcagc ttgtcccagc
     2401 cgaaatggcg ctcgatgagt tcgtaggtct tgagcatgcc gttgcgctcg gaaatcgtga
     2461 cccggcccga atagaacgca agctgcagcg cgcgcttcga cggtttgcgg ctcgaccaaa
     2521 gatgctcctt ttcggtgagc acgtcgtcct cgatgtcacg aatggtcagc gcaccctggc
     2581 gcagcagccg catcaccttg cgggtgtcgg ccggcttgac ggaggcgaac catctgtggc
     2641 cctcgcgccg gtgcaggcgc atctccggga tgaagtagcg gaagtccctt gtcggcacat
     2701 aggacagcgc atgggtccag tattcaaaca cgctgcggtc gacactctgc gcctggcgca
     2761 gatcgtcgcg ccggtagtcg gggatacgat tccagagaat gtggtggtga cagcgctcga
     2821 tcacgttgat ggtgtcgatc tgcacatagc cgagatgggc gaccgcatcc gccacggcct
     2881 gcggccctgc accgaacggc accggcaggt cgaggcgctg ggcggcaagc cagatccgcc
     2941 gggcctcacg cttgcttaag ggaaagggct tgggcgcggg agacattgcg cggcaatgta
     3001 gcgggatttg cgcggacgcc aagaacggac ccgagcagac atgaagaaaa gccgcactgc
     3061 ttgggtcgca gcgcggcaag ccaaaggaaa aatccgtccc cggcttactt cgccatgccc
     3121 atcaaggctt cggccgttcg ggaatgctgc cggttgcgat atcggcatcg cagttggcct
     3181 tgccgcgctg cgaggcctcg caccgataga catggccggt cagccggtcg atcagccagg
     3241 cgctttcttc gcttgggcct tcgaggccga cgtaacgagt cccaatcgcg ttgaccagcg
     3301 tggcgagaag aatcgccgcc gcaaccatgg ctgcgccgat caggacgggc attgtactga
     3361 tgatgatcgt gcgctcagcc gcgctgcgtt gcccctggta ttcgatatgt cgtttcgctg
     3421 acgtaatcac ggcccctgct tcaccttttc ttctgggctt ttcttgtcct cttggctgtc
     3481 accatcttga gaacctatct ggccaatttg ttgttcgcag gtcggctgct ttaagatgag
     3541 tcgagcggat tcgacgttac gccgaatcca aagctccatt cgcattattg atttgcaagc
     3601 gacactttga tcacaacttt cgcaaaaaaa ggctgtcccg acggagccaa acatcacagg
     3661 gcaaaaaaag aggcggcgcg ggccgcctca agtttgggga gacgaactca aactcagtgt
     3721 cggaacaatc cacccatgaa gccaaagaag ccgccgggtg cgccgccgcc gccaccgcga
     3781 taatagcgcc gcccgccacc gccgccgccg ctgtgacgca cgtgcccgga gccctcggag
     3841 gaggagtccg tcgaggcgac ccgggcggaa acgatctcgg agagcagcgc cttatgctgc
     3901 agattgttga ggtagccggt cgcgggatag ccgcgcgcag cctgccagcg acggatcacg
     3961 gaacgggttt ggtcgtcgaa cttgcccgtc accttgacgt cgaagccgag cccggtgagg
     4021 cggcgctgca cgtcgcggcg ctggcccttg tcgaggccga tctgatcctc ggaggtctgg
     4081 tcagcctcct cggtgaacgt cgcgggatcg acctcggtgg tcaggttccg cgtcgtggtc
     4141 gacgggccgt tctgcaggga ggcgatccgg gcaagcgcca gcgacttgaa tgtgccgttc
     4201 ggatagctgg tgagataggc attaagctct tccggcttgt tggaatcctt cacggagcgc
     4261 cagaactcga gctcgacgtc ggaggcgggc ccggggggcg tgctcggcgc ctcggccttc
     4321 tcggagggga caccgagcgg gttgagatac acactgccga tcaggttggt gtggccccag
     4381 gggagttggt tcttgttggt ctcctcgttg acctgggcgc ggactttggt catcgcctgt
     4441 tgaatctcga cgccgggctg ggtgatgttg gcgacgagcg cgcgggtgaa cggcgagttg
     4501 gtgccgttct cgccgtcaag cgcggtctgg cccgggccgg tggcgaaagc gatcagcgtg
     4561 ccctcgcccg acttcatctc ggcaaggcct gactgaacgg atacgctgcg tgtcgccttc
     4621 gcggagcgga tctttgcggc gaaaggattg tcgcggcagg cgtcgaggaa caccagcttg
     4681 accttggcat ccgccatggt ctgttcgagc gtcacgtcga cgttgatggc ggcaccg
//
LOCUS       DATLJT010001660         3427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_859426,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001660 DATLJT010000000
VERSION     DATLJT010001660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859426"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /locus_tag="VM910_37770"
     CDS             <1..2373
                     /locus_tag="VM910_37770"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HVI68289.1"
                     /translation="INVNITLLFSQKVYLQVAKAYLAGLEKYVAKGGDPSHVASVASF
                     FVSRIDTLADKQLDEKIAKANDPTEKARLAALKGKVAIANAKLAYQEYKQLFAGAPWD
                     KLAAKGARPQRLLWASTGTKNKDYSDVLYVEELIGPNTINTMPPATLDAFRDHGKVRD
                     TLEEDIDGARQVLAELEKSGISLDAITEELITDGVKLFADAADKLYGAVASKRASVLG
                     TGVDRQKLALGMTIAKAVEKNAEQWRASAKVRRLWHKDKSVWTGDDEDKWLGWLTSAA
                     SADIADYEDFAQRVKGQKFTDAVVLGMGGSSLGPEVLAETFPKKSGFPRLHVLDSTDP
                     AQVRAMEKAVNLAKTLFIVSSKSGGTTEPNAMKDYFYDRVSKTIGAEAAGHRFIAVTD
                     PGSSLEKVAIKQGFACIFHGEPTIGGRYSVLSPFGLVPAAAAGIDVRALIDHALSMVR
                     SCGADVPPQENPGVLLGLAMGLAGLEGRDKVTILSSKKVADFGAWAEQLIAESTGKEG
                     KGLIPIDGEPLGEAAVYGDDRFFIDIRTEGEDDAAHDERLAAVEQAGHPVVRIVMKSI
                     DHIGQEFFRFEMATAVAGAILGINPFNQPDVESAKIKTRELTAAYEKTGALPPEKPAI
                     STAEADLFTDDANASELRRLGADGSIDTWLKAHLSRAHVHDYVAVLAYIARNAGHIDA
                     LQSMRLRVRDRRHLATCAEFGPRFLHSTGQAYKGGPDSGVFLQITADDAKDLPVPGQK
                     ASFGVIKAAQARGDFDVLTERGRRALRVHLKGDLEAGLKMLDAAIADALN"
     gene            2441..>3427
                     /gene="gnd"
                     /locus_tag="VM910_37775"
     CDS             2441..>3427
                     /gene="gnd"
                     /locus_tag="VM910_37775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HVI68290.1"
                     /translation="MQLGMIGLGRMGGNIVRRLMKHGHTTVVYDKDEKAVAALAADGA
                     AGAKALEDFVAKLTKPRTAWVMLPAGRITEETIAALAKLMEADDVIIDGGNSFWQDDV
                     RRGRTLKQRGIHYLDVGTSGGIWGIERGYCMMIGGDKAVVDRLDPIFAALAPGIGDIP
                     HTPHREGRDPRVEQGYIHAGPVGAGHFVKMVHNGIEYGLMQAYAEGFDILKNADIEAL
                     PAEHRFTLDVADIAEVWRRGSVIPSWLLDLTAAALAENHTLDTYSGFVEDSGEGRWTV
                     NAAIDEAVPAEVLTAALFARFRSRKDHTFAEKILSAMRAGFGGHREPKKPTSK"
BASE COUNT          694 a         1087 c         1096 g          550 t
ORIGIN      
        1 atcaacgtca acatcacgct cctgttctcg cagaaggtct atctgcaggt cgcgaaagcc
       61 tatcttgccg ggctggaaaa atacgtcgcc aaaggcggcg acccttcgca tgtcgcaagc
      121 gtcgccagtt tcttcgtcag ccgcatcgac acgctggccg acaagcaact cgacgagaag
      181 atcgccaaag ccaacgaccc gacggaaaag gcgcggctcg cggcgctgaa gggcaaggtt
      241 gcgatcgcca acgccaagct tgcctatcag gagtacaagc agctctttgc gggcgcaccc
      301 tgggacaagc tcgccgccaa gggcgcgagg ccgcagcggc tgttgtgggc ctccaccggc
      361 acgaagaaca aggactatag cgacgtcctc tatgtcgagg aattgatcgg gcccaacacc
      421 atcaacacca tgccgccggc gacgctggat gcgttccgcg accacggcaa ggtgcgcgac
      481 accctggagg aagacatcga cggcgcgcgg caggtgctcg ccgaacttga aaaatccggg
      541 atctcgctcg atgcgatcac ggaagaactc attaccgacg gcgtcaagct gttcgcggat
      601 gctgcggaca agctctatgg cgcggtggcc tccaagcgtg cgtccgtgct cggcactggc
      661 gtcgaccggc aaaagctcgc cctcggcatg acgattgcga aggcggtgga gaagaacgcc
      721 gagcaatggc gggcctccgc caaggtgcgc aggctctggc acaaggacaa atcggtctgg
      781 accggcgacg acgaagacaa atggctggga tggctgacca gcgcggccag tgccgacatc
      841 gccgactacg aagatttcgc gcagcgggtg aagggacaaa aattcaccga cgcagtcgtg
      901 ctcggcatgg gcggatcgag cctggggccg gaagtgctgg ccgaaacttt cccgaagaag
      961 tccggctttc cgagattgca tgtcctggat tcgaccgatc cggcgcaggt gcgcgccatg
     1021 gagaaggcgg tcaatctcgc caagaccctg ttcatcgtct cctccaaatc cggcggcacg
     1081 accgaaccga acgcaatgaa ggattatttc tacgatcgcg tgtcgaagac cattggcgcc
     1141 gaagcagccg ggcatcgctt catcgcggtg accgatcccg gctcgtcgct ggagaaggtc
     1201 gcgatcaagc agggctttgc ctgcatcttc cacggggagc ccacgatcgg cggccgctat
     1261 tcggtgctgt caccattcgg tctggtgccg gctgcggccg caggcatcga cgtgcgcgcc
     1321 ctcatcgacc acgccctcag catggtgcgc tcctgcggcg ccgacgtgcc gccgcaggaa
     1381 aatcccggcg tgcttcttgg gctcgcgatg ggtcttgcgg ggcttgaggg ccgtgacaag
     1441 gtgacgatcc tgtcctcgaa aaaggtcgcg gacttcggcg cctgggcgga gcaactgatc
     1501 gcggaatcca ccggcaagga aggcaagggc ctgatcccga tcgacggcga gccgctcgga
     1561 gaggcggccg tttacggcga cgaccgattc ttcatcgaca tcaggaccga gggcgaggac
     1621 gacgctgcgc acgacgagag gctcgccgca gtcgagcaag ccggtcatcc cgtggtgcgg
     1681 atcgtcatga aatcgatcga tcacatcggt caggagtttt tccgcttcga gatggcaacc
     1741 gcggttgcgg gcgcaatcct cggcatcaat ccctttaacc agcccgacgt cgagtcggcc
     1801 aagatcaaga cgcgcgaact gaccgctgcc tacgagaaga ccggtgcctt gccgccggag
     1861 aagcccgcaa tatcaacggc cgaggccgat ctcttcacag acgacgccaa tgccagcgaa
     1921 ctgcgcaggc tgggcgccga cggcagcatt gacacctggt tgaaagccca tctttcccga
     1981 gcgcacgtgc acgattacgt ggctgtgctc gcctatatcg cgcgcaacgc cggccatatc
     2041 gacgccctgc aatcgatgcg gctcagggtc cgcgacaggc gccaccttgc aacctgcgcg
     2101 gagttcggtc cacgcttcct gcattcgacc ggccaggcct acaagggcgg ccccgactcc
     2161 ggcgtcttcc tgcagatcac tgccgacgac gccaaggacc tgcccgttcc cggccagaag
     2221 gcaagctttg gcgtgatcaa ggccgcgcag gcgcgcggcg atttcgacgt cttgaccgaa
     2281 cgcggccgac gcgcgctgcg ggtgcatctg aagggcgacc ttgaggcggg cctgaagatg
     2341 ctcgacgccg cgatcgcgga cgcgttgaac tagagcacga tcccgaagag caaggcgtcg
     2401 gttttcggat gcgatcgtgc tccacaaagg aaaggaaaac atgcagcttg gcatgatcgg
     2461 cctgggccgc atgggcggca acatcgttcg ccggctgatg aagcacgggc acacgaccgt
     2521 cgtctacgac aaggacgaaa aagccgtcgc ggcccttgcc gccgacggcg cagcgggcgc
     2581 gaaagcactg gaggatttcg tcgcaaagtt gacgaagccg cggacggcgt gggtgatgct
     2641 gcctgccggc aggatcacgg aagagaccat cgcggcgctg gcgaagctga tggaggcgga
     2701 cgacgtcatc atcgacggcg gcaacagctt ttggcaggac gacgtccgcc gcggcaggac
     2761 gctgaaacag cgcggcatcc attatctcga cgtcggcacg tcgggcggca tctggggcat
     2821 cgagcgcggc tattgcatga tgatcggcgg cgacaaggcg gtggtcgacc ggcttgatcc
     2881 gatcttcgcc gcgcttgccc ccgggatcgg cgacattcca cataccccgc accgcgaagg
     2941 ccgcgatccc cgcgtcgagc agggctatat tcacgcgggt cccgtcggcg ccggccattt
     3001 cgtcaagatg gtgcacaacg gcatcgaata cggcctgatg caggcctatg ccgaaggttt
     3061 cgacattctc aagaacgctg atatcgaggc gctgccggcg gaacatcgct tcacgctcga
     3121 cgtcgccgac attgccgagg tctggcggcg cggcagcgtc atcccgtcct ggctgctcga
     3181 cctcaccgca gcggcactcg ccgaaaatca cacgctcgac acctattcgg gctttgtcga
     3241 ggattccggt gaaggccgct ggaccgtcaa tgccgcaatc gacgaggcgg tacccgcgga
     3301 agtcctgacc gcggccttgt tcgcgcgctt ccgctcgcgg aaagaccaca ccttcgccga
     3361 aaagattctc tcggcaatgc gcgccggctt cggcgggcat cgggagccca aaaagccaac
     3421 gtcaaag
//
LOCUS       DATLJT010001661         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1323192,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001661 DATLJT010000000
VERSION     DATLJT010001661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323192"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..330
                     /locus_tag="VM910_37780"
     CDS             46..330
                     /locus_tag="VM910_37780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HVI68291.1"
                     /translation="MALRRFIIERDIPKVGTFEREQLRGAAAKSNGVLRQLGPDIQWL
                     ESYVADNKTFCVYLAKDEDIIRKHAEISGFPATKITEVRKMIDPTTERLD"
     gene            335..745
                     /locus_tag="VM910_37785"
     CDS             335..745
                     /locus_tag="VM910_37785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI68292.1"
                     /translation="MRRREFITLLGGAAATWPLMAYAQQPTMPVIGFLVSTSADGFTE
                     RLRKFSQGLKEQGFVEGENLVILYRFADNEADRLPALAADLVRRKVAVIATGGPPATF
                     AAQAATATIPIAFLVADDPVRSVSPQASRDRAAT"
     gene            742..1317
                     /locus_tag="VM910_37790"
     CDS             742..1317
                     /locus_tag="VM910_37790"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68293.1"
                     /translation="MTGINVFNAEVAAKRLELLRDLVPGVTRIAVFVNSADPEMMETQ
                     LRDVGTAARAMGLHIQTHDANTSAEIDAAFKSFGRERPDAVFVPTSPFFNGRRVQLAQ
                     LAAFHHLPATYALRDYAEVGGLMSYGSNIVDGYRQIGIYVGRVLKGAKPADLPIVQAN
                     KFELVINMHTAHMLDLVVPPSLLAIADEVIE"
     gene            1314..1676
                     /locus_tag="VM910_37795"
     CDS             1314..1676
                     /locus_tag="VM910_37795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68294.1"
                     /translation="MRRSEFITLLGGAAASWPLAARAQQGERMRRIGVLTVNAESDQE
                     YRASIAQFVQGLASFGWTEGRNIQIDHRYAAGDVGRMHVFAKELVGLAARKQVEREGL
                     IPSPKRREDARRQRPRPF"
     gene            complement(1739..1825)
                     /locus_tag="VM910_37800"
                     /pseudo
     CDS             complement(1739..1825)
                     /locus_tag="VM910_37800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
     gene            2072..3255
                     /locus_tag="VM910_37805"
                     /pseudo
     CDS             2072..3255
                     /locus_tag="VM910_37805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713295.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
BASE COUNT          678 a          989 c         1038 g          550 t
ORIGIN      
        1 aaccccgcgc ggctgggtat aacacggagt cagggagaga cggatatggc acttcggagg
       61 tttatcatcg aacgggatat tccaaaggtc ggcaccttcg agcgcgaaca gcttcgcgga
      121 gcagcagcga aatcgaatgg ggtattgcgc cagcttggtc ctgacatcca gtggcttgaa
      181 tcctatgtcg ccgacaacaa gacgttctgc gtctatctcg ccaaagatga ggacatcatc
      241 cgcaagcacg cggagatcag cggttttccg gcaacgaaga ttactgaggt ccgcaagatg
      301 atcgatccaa cgacggaacg gctagactga tcaaatgcga cgacgcgagt tcatcacgct
      361 ccttggcggc gcggcagcta cgtggccgct catggcatac gcgcagcagc cgacaatgcc
      421 ggtgatcggg ttcctcgtta gcacgtcggc tgacgggttt actgagcgcc tgcgcaaatt
      481 cagccaaggc ctgaaggagc agggcttcgt cgagggcgag aacctggtga tcttataccg
      541 cttcgctgac aacgaggccg accggttgcc ggcgctggcg gccgacctgg ttcgtcgcaa
      601 ggtcgccgtg atcgccactg gaggtcctcc ggcgacattt gcggcccagg cagcaaccgc
      661 cacgattcct atcgcgttcc tggtcgccga tgatccggtg cgctccgtct cgccgcaagc
      721 ctctcgcgac cgggcagcaa catgacggga atcaatgttt tcaatgccga agtagcggca
      781 aagcggttag aacttctgcg tgacctcgtc cccggcgtga cccgcattgc ggtattcgtc
      841 aattcggccg accctgagat gatggagaca cagctgagag acgtgggcac ggctgctcgc
      901 gccatggggc tgcacatcca gacgcacgac gcaaacacca gtgctgagat cgatgctgct
      961 ttcaaatcct ttggtcgcga gcggcctgat gcggttttcg ttccgacaag cccgtttttt
     1021 aacggccggc gcgtccaatt ggcacaactg gcggcattcc atcacctccc agcgacgtac
     1081 gcattgcgtg actacgccga agtcgggggt ctgatgagct atgggtccaa catagtcgat
     1141 ggttaccgtc agattggcat ttatgtcggt cgcgtcctca aaggcgcgaa gcctgcggat
     1201 ttgccaatcg tgcaagcgaa caagttcgaa ctggtaatca atatgcatac cgcgcacatg
     1261 ttagatcttg tcgtgccgcc atcgctgctg gcgatcgccg acgaggtgat cgagtgaggc
     1321 ggagcgagtt catcacgctg ctcggcggcg cagcagcatc gtggccgctc gcagcgcgcg
     1381 cgcagcaagg cgagcgcatg cgccggatcg gcgtactcac ggtcaatgcc gaaagcgacc
     1441 aggagtaccg ggctagcatc gcgcagtttg tgcagggact cgcctccttt ggatggaccg
     1501 agggacgcaa tatccaaatc gaccatcgct acgccgccgg cgatgtcggg cgcatgcacg
     1561 tcttcgcgaa ggaactggta ggcctggcgg cccggaaaca ggtcgagcgc gaaggtctca
     1621 tcccaagccc caaacgccgg gaagacgcgc gccgtcagcg gcccaggccg ttctgagttt
     1681 tctgccaagt cccgccggat gcctgctagg cggatccgat caggctacag gttacgcgct
     1741 tcatctcacg aaaaacgccc tcgcgttgca tcttcctctt taggaccctg agggcttgac
     1801 cgagcttgtt gtcgcgaacg aacagatctc cgcttcgagg ctcgccgatc gagcctgtgc
     1861 cttctcaaaa ccgcgcggcc ggtgaactta gtggctttcc ggcgcatttg tttagagaaa
     1921 tcgaaagact cttcttcaga tgagttttag gtaggcgcag ccgacccttt aggctatcgg
     1981 ctacgcgaga ggaccgcgat tcggcgcggg ctcagtgccg gctgtgaggc tcatggcaag
     2041 caagaccgag gagacgccgc agaaggaaga tatgccggag aaggggggtc ccgagaccct
     2101 gccgcatgac ctgtcgggcg cggcgatcac gaagctcatc cgctccgcca agaagcgcgg
     2161 ctacctcacc caggaccaga tcaactcggt attgccctcc gaggaggtca attccgagca
     2221 gatcgaggat gtgctggcca tgttccgcga gatgggcgtg aacgtggtcg agacccagga
     2281 gacgagcaaa gagggagcgg agccgcgcga agaggaagaa gagccgcgcg aagaggccga
     2341 cgaagagggc gagagcgagg gcggtgacct ggtcgaggtc cagcagaagg tccccgccaa
     2401 ggccgaggcc aaagacccga ccgaacgaac cgatgatccg gtgcgcatgt atctgcgcga
     2461 aatgagcccg gtgaagctgt tgtcgcgcga gggcgagatg gcgatcgcca agcgcatcga
     2521 ggccggccgc gaggcgatga tcgccggact gtgcgagagc ccgctcacct tccaggccat
     2581 catcatttgg cgcggcgagc cggcaaggtc ttcccgcgcg acatcgtcga tctcgaagcg
     2641 acctatgccg gtcccgacgc caaggccatg ccggcgccgg tgatcggccc cgacggccaa
     2701 ccgagcgtga cggcggcggg agcgccgccg gttcccagct cccccgtgcc ggcgcagatg
     2761 ccggtgccgt cggcagcgcc aacgggggcg accccgttca atggagcgcc cgagcgcggc
     2821 aacggggaga atggcgaggc cggcgagacc ccgcccggcg aaggcgattt cgatgacgac
     2881 gatgtggaga actcgctctc gcttgccgcc atcgaggccg agctcaagcc caaggtggtc
     2941 gagaccttcg acaacatcgc cgacgcctac aaacggctgc gccgcctgca ggaccaggac
     3001 atccagaaca agctgcgcaa cgattcgctc tcgccggcgc aggaacgccg ctacaagaag
     3061 ctcaaggacg acatcatcag cgaggtgaag tcgttacggc tcaatcaggc gcgcatcgac
     3121 gcgctggtcg agcagctcta cgacatcaac aagcgcctgg tcggctacga aggccgcctg
     3181 atgcgactcg ccgagggtca cggcgtcgca cgcgaggatt tcctcaagaa ctaccagggc
     3241 tacgagctcg atccg
//
LOCUS       DATLJT010001662         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1090011,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001662 DATLJT010000000
VERSION     DATLJT010001662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090011"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..306)
                     /locus_tag="VM910_37810"
     CDS             complement(85..306)
                     /locus_tag="VM910_37810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68295.1"
                     /translation="MTAKMKRREFITLLGGAAAWPLAARAQQAAMPVQQASQGHGSPR
                     MAGFRQGLNEIGPTIVFIGELDLPLADCA"
     gene            complement(303..1313)
                     /locus_tag="VM910_37815"
     CDS             complement(303..1313)
                     /locus_tag="VM910_37815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68296.1"
                     /translation="MPYPEPGWNMKRREFLSVLGGATAWPLAARAQQPAMPVIGFLDT
                     RSPDAMADRLRGFRQGLREIGYVEGDNATIVYRWAENKIDRVPELMADLIRRQAAVIL
                     AGGNIAAIFAAKAATTTVPIIFIVPEDPVRLGLVASLARPGGNLTGINFFNAELVAKQ
                     LELLHELLPRAARVAIFVNPTSASNTESMERDAPPAARAMGLQIQFLRASNSREIDTA
                     FATFARELPDALFVGNDGFFNSRRVQFVHQASHHRLPAIYTGREFSEIGGLMSYGTNI
                     ADAFRQAGVYAGRILKGAKPADLPVVQSSKFELVINHQTARMLGLTVPDKLLVAADEV
                     IE"
     gene            complement(1330..2301)
                     /locus_tag="VM910_37820"
     CDS             complement(1330..2301)
                     /locus_tag="VM910_37820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68297.1"
                     /translation="MKLPRRQFMHLAAGAAALPRVAWAQAYPARAVRVIVPFAPGGLT
                     DVFARLAAQKLTEHFGRQFYVENISGASGNIGTGQAARSAPDGYTLLFAYTSHVVNPS
                     VFAKIPYHPVKDFAPVTLAVGSATVLSVNPSLPARTVKELVALIKANPGKYNIASPGA
                     GATTHLTGEQFRASLALDLAQVPFNGAGPAIASVIAGHTQIMFSAVASAASQIKAGQL
                     RPLAVTGKTRSAILPEVPTTTEAGYPDIEGDGWVGVLVPAGTPKEIITTLHREMVRII
                     ALPDMKERLPTLGFEPIGNTPEEFEAQIKAEIEKWARIVRDANVKTQ"
     gene            complement(2313..2468)
                     /locus_tag="VM910_37825"
                     /pseudo
     CDS             complement(2313..2468)
                     /locus_tag="VM910_37825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
BASE COUNT          455 a          803 c          820 g          508 t
ORIGIN      
        1 cgggggcggc gtcccatcta ccggccacac atacgctttc atcatcggtt ctccaccgtc
       61 tgctgcgcgt cgaaacggcc gtttctatgc gcaatctgcg agcggcaagt ccaactcgcc
      121 aataaaaacg atcgttgggc caatctcatt taagccttgc cgaaacccgg ccatgcgcgg
      181 tgagccgtgg ccctgcgagg cctgctgcac cggcatcgcc gcctgctgcg cgcgcgcggc
      241 gagcggccac gccgccgcac cgccgagcaa cgttatgaac tcgcggcgct tcatcttcgc
      301 ggtcactcga tcacctcgtc ggcggccacg agcagcttgt ccggcacggt aaggccgagc
      361 atgcgcgcgg tctggtggtt gatgaccagt tcgaattttg acgattgcac gactggcagg
      421 tctgctggtt tggcgccctt aaggatgcgg ccggcataga cgccggcctg ccgaaaggcg
      481 tcggcaatgt tggtgccata gctcattagt ccgccgattt cggaaaattc tcggcccgtg
      541 tagatcgcgg gaagccgatg gtgcgatgcc tggtgaacga attgcacacg tcggctgttg
      601 aagaagccgt cgttgccgac gaagagggca tcgggcagtt cgcgcgcaaa tgttgcaaac
      661 gccgtatcga tctcccggct gttgctggcg cggaggaact ggatttgcag tcccatggcg
      721 cgcgcagccg gtggcgcgtc tctttccatg gactcggtat tcgaagcact cgtcggattg
      781 acgaagatag cgacgcgggc ggctcggggt aacagctcat gcaagagttc cagctgcttc
      841 gccaccaact cggcattgaa aaaattgatg ccggtcaggt tgccgcccgg ccgggctagg
      901 ctcgcgacaa gaccgagccg cacggggtct tcggggacga tgaagatgat cggcaccgtc
      961 gtggttgccg cctttgcagc aaagatggcg gcaatatttc cacccgcgag gatcacggcg
     1021 gcctgacggc gaatcagatc ggccatcagt tcgggcactc gatcgatttt attttctgcc
     1081 cagcggtaga cgatcgttgc gttgtcgcct tccacatagc cgatctctct gaggccctga
     1141 cgaaatccgc gcagacggtc cgccatagcg tcgggcgacc ttgtgtcgag gaatccgatc
     1201 accggcatcg ctggctgctg cgcgcgcgcc gcgagcggcc aagctgttgc gccgccaagc
     1261 acactgagaa attcgcgccg cttcatgttc cacccaggct cgggataagg cattgatggc
     1321 gttcagccct tactgggtct tgacgttcgc atcgcgaacg atccttgccc acttctcgat
     1381 ttctgccttg atctgcgctt cgaattcctc gggcgtgttt ccgatcggct cgaacccgag
     1441 cgtcggcagg cgctccttca tgtccggcag cgcgatgatc cggaccatct cgcggtgcag
     1501 cgtcgtgatg atctctttcg gcgttccggc cggcaccagg acccccaccc aaccgtcccc
     1561 ctcgatatcc ggatagcccg cctcggtcgt ggtcggcact tcgggcagta tcgccgagcg
     1621 cgtcttgccc gtgaccgcga gcgggcgcaa ctgacctgct ttgatttgtg acgcggccga
     1681 cgccaccgca ctgaacatga tttgggtgtg acctgcgatc accgaggcaa tcgccgggcc
     1741 agcgccattg aatgggacct gcgcgagatc gagcgcgagc gatgcgcgga actgctcgcc
     1801 agtgagatgc gtcgtggccc cggcccctgg tgaagcgatg ttgtacttgc cagggttggc
     1861 tttgatcagt gcaaccagct ccttgacggt ccttgccggc aacgagggat tgaccgaaag
     1921 cacggttgcc gagccgaccg cgagcgtgac cggcgcaaag tctttgacgg gatgataggg
     1981 aatcttggcg aagacggacg ggttcacgac atgcgaggtg taggcgaaca ggagggtgta
     2041 gccgtcgggt gcgctcctcg ccgcctgacc ggtgccgatg tttccgctgg caccgctgat
     2101 attctcgacg tagaactgcc ggccgaaatg ctcggtcagc ttctgggcag cgagccgcgc
     2161 gaacacgtcg gtcaggccgc cgggcgcgaa tgggacgatg actcgcaccg cccgtgctgg
     2221 ataagcttgc gcccacgcga cgcgcggcag cgcggcagca cccgctgcca gatgcataaa
     2281 ttgtcgacgg ggaagtttca tcgtgggtcc cctcattcga tcacctcgtc ggcgcgggcg
     2341 agcagcgtcg ccggcacttc caggccaagc gccttggcgg tcttgaggtt gatgaccaac
     2401 gcgtacttgg taggcgcttg caccggcagg tcggcgggct tttcgccctt gaggatgcga
     2461 tcgacatact cgccacacag taggcaccag ctttggattg cggcaggaca tcggaggcga
     2521 caggccaaca gcctggacgc cccccgtcag tgcttttctg gatacttgct gatgaaccca
     2581 tcgcgg
//
LOCUS       DATLJT010001663         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4078392,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001663 DATLJT010000000
VERSION     DATLJT010001663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4078392"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..457)
                     /locus_tag="VM910_37830"
     CDS             complement(242..457)
                     /locus_tag="VM910_37830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HVI68298.1"
                     /translation="MRYRSNSDLPASVQAHLPPHAQDIYREAFNHAFAAHAGDPYQEE
                     RAHRIAWAAVKRSYVKVGGSWIGRDRT"
     gene            complement(597..1127)
                     /locus_tag="VM910_37835"
     CDS             complement(597..1127)
                     /locus_tag="VM910_37835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVI68299.1"
                     /translation="MAETATKLPVGKQEKTDRPAEWRPFESLRREVDRLFEDFQLGSW
                     RSPFGRSLFDVQPFWRGEVGWGKAPAVDIVEKEKAYEITAELPGMDESNIDVTFSDGT
                     LTIKGEKRDEKEEKKKDYYLSERRYGSFQRSFGVPDGVDADKIEANFKNGVLTVTLPK
                     TAQAQKSEKKIAIRAA"
     gene            complement(1223..1915)
                     /locus_tag="VM910_37840"
     CDS             complement(1223..1915)
                     /locus_tag="VM910_37840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI68300.1"
                     /translation="MKAADVMVRNVITMGPDACVQDVAHVLLTARISGVPVVGPKGEL
                     VGIVSEGDLMCRVEAGTGRRRPWWLAIFTGKEALAMEFIREHSRKVADEMTREVITAA
                     LDTPLWKIANLLEKNAIKRVPIVEDGKVVGIVSRANLLQALASLSKRAEVATRTDDSQ
                     IRDTVLTQLNAEPWTRPSLINVIVQGGTVELWGIVDSASEKKAVRIAAEVTPGVRAVN
                     DNLIIRPAQAGY"
     gene            2046..2369
                     /locus_tag="VM910_37845"
     CDS             2046..2369
                     /locus_tag="VM910_37845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HVI68301.1"
                     /translation="MRAKRLILPAVIVLLVPTGHGAGQEIGQSSRGLVLAQRLCAECH
                     AVQREYSRSPNANAPRFQAIASIPGMTATALSAALNTSHRAMPNIMLAADEQADIITY
                     ILSLK"
BASE COUNT          420 a          769 c          687 g          499 t
ORIGIN      
        1 tccgtggcat tggaggcctc atccggcatg gtcaccatgt ccttggcatc gaactcgcgc
       61 gccttgacca cgatgaaaca gaccttttcg ggagagatcg agagtgccgg agtggctttg
      121 gtcgcggccg catcttcagc cggcgacgcc ttggtttcag gactgcctgc gcgcatgggt
      181 ctttctcctg gctcttgttg cccaaaaata ccccgcgggg cggcagagac gttgagatag
      241 gtcaagttcg gtcgcggccg atccagctgc cgccgacctt cacgtacgag cgcttgacgg
      301 ccgcccaggc gatgcggtgc gcgcgctcct cctggtaggg atcacccgca tgcgccgcga
      361 aggcatgatt gaaggcctcg cgatagatgt cctgggcgtg cggcggaagg tgcgcctgca
      421 ccgaagcggg cagatcgctg ttggaacggt atcgcattgc cgtcacctcg ccaaggcgac
      481 ccgagcgcag gggcctcact cgtctacaaa aaagcggccg ccgacttctc tcaggcggcc
      541 tagggctgcg ttgcaaagtc ggcagatgcc gactttgcag gttcgatctc gacaagtcag
      601 gccgctctga tcgcgatctt cttttcgctc ttctgtgcct gcgcggtctt cggcagcgtg
      661 acggtgagca cgccgttctt gaaatttgcc tcgattttgt cggcgtcgac gccgtccggg
      721 actccgaacg atcgctggaa cgagccatag cggcgctcgg acaggtaata atccttcttc
      781 ttctcctcct tttcgtcccg tttctcgccc ttgatcgtca gcgtgccgtc ggagaacgtg
      841 acgtcgatat tgctctcgtc catccccggc agctccgccg tgatctcgta ggccttttcc
      901 ttttcgacga tgtcgacggc tggcgccttg ccccagccga cctcgccacg ccagaacggc
      961 tgaacgtcga ataggctacg cccgaacggc gagcgccagg agcccagctg aaaatcttcg
     1021 aacaggcgat cgacttcccg ccgcaagctc tcgaacggcc gccactcggc cggacgatca
     1081 gtcttctctt gctttcccac cggcagtttc gttgcggttt cagccatgtc acttctcctc
     1141 actttgatca tgagcggtat atggtgaact gatacaaagc cagcaaccgc gggtgttttg
     1201 acggaaactc aaatcgtctg cgtcagtacc ccgcctgcgc cggacgaata atcagattgt
     1261 cattgacggc gcgcaccccg ggggtgacct cggcggcgat gcggacggct ttcttctctg
     1321 atgccgagtc cacgatcccc cacagctcga ccgtgccgcc ctgcacgatc acgttgatca
     1381 acgagggcct cgtccacggc tctgcgttga gctgcgtgag cactgtatcc cggatctgcg
     1441 agtcgtcggt ccgtgttgcg acttcagccc gcttgctcag actggccaag gcttgcagca
     1501 ggttcgcacg gctgacgatg ccgaccacct tgccgtcttc cacgattgga acgcgcttga
     1561 tcgcgttctt ctcgagcaga tttgcgatct tccagagcgg agtgtcgagg gccgcggtta
     1621 tcacctcgcg cgtcatctcg tcggcgacct tgcgcgaatg ctcccttatg aattccatcg
     1681 ccagcgcctc tttgcccgtg aagatggcga gccaccacgg tcgccggcgg ccggtgccgg
     1741 cctcgacgcg acacatgaga tcgccttcgc tgacgatacc gacgagctcg cccttcggcc
     1801 cgacgaccgg aacgccactg atccgcgcgg tcagcaaaac atgcgcgacg tcttgaacgc
     1861 aggcatcggg acccatcgtg atcacgttcc taaccattac gtcggcggct ttcatggcac
     1921 ctctccattg cgcgaccagc ttctatcgct cgcaggcgag ttctttgatc tgaatcaaga
     1981 aacgggcaca gtcttccgtg gttcgctggc gccaatttcg aggatgcggg gcagcggagg
     2041 ttcgaatgcg tgcgaagcgg ctgatactgc cggcggtcat cgtcctgctg gtgccgacag
     2101 ggcatggcgc gggacaggag atcggacaat cgagccgcgg cctcgttctg gcccaacggt
     2161 tatgcgcgga atgtcatgcc gtgcaaagag aatactcgcg atcgcccaac gcgaacgcgc
     2221 ctcgcttcca ggctattgcc tccatccccg gcatgaccgc gaccgcgcta tcggccgcgc
     2281 tcaatacatc acatcgcgcg atgcccaaca tcatgctcgc agcagacgag caggcagaca
     2341 tcatcaccta tattctgagc ctcaaatgaa tttcg
//
LOCUS       DATLJT010001664         6468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2471117,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001664 DATLJT010000000
VERSION     DATLJT010001664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471117"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VM910_37850"
     CDS             <1..229
                     /locus_tag="VM910_37850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HVI68302.1"
                     /translation="FQIVIMMKGWARFMYEDKPTLVSAGDVVHQRPGIVHYLFDYSPD
                     MEYLEIVSPADFKTVDMPPATDKVPPVTPWK"
     gene            complement(626..847)
                     /locus_tag="VM910_37855"
     CDS             complement(626..847)
                     /locus_tag="VM910_37855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68303.1"
                     /translation="MATRERRLAEFDGAGKPPPGTAVQVLCEDQSGTYLLPFACHYVD
                     GQWHNYESGGALEATVVGWRMPRRHYTRA"
     gene            1151..1474
                     /locus_tag="VM910_37860"
     CDS             1151..1474
                     /locus_tag="VM910_37860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68304.1"
                     /translation="MLMQAELRAQDIPGIEICTVEKTMERRTSCLQSNVEFLQRTVTK
                     LASDYQLKLDAANRQIEALKAAAVALQKTVADLQTSQKQMTDDLKKKADASPAKDAAP
                     TKQGK"
     gene            complement(1488..2591)
                     /locus_tag="VM910_37865"
     CDS             complement(1488..2591)
                     /locus_tag="VM910_37865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HVI68305.1"
                     /translation="MQITNVRAHHLRIPYDVGVAKFRHGTSGLSELDMVVVEVSTDQG
                     VTGWGDAFSYVCPRTTCTAIDEMIAPQVRGREVPEADKIPAFMEEIQRNLHLFGRYGI
                     TMFAISGLDIALWDIAARVKGVPLHQLIGGARRLKIPAYASLLRIGNEQSIAGECEAA
                     RRAGYGAIKLHEITLPAVFAARKAIGDGVPLMVDMNCPLDGTAAIAFAQACRAADPMF
                     LEEPVWPPEDFVTLAEVRAKGGLDIAAGENACTAYQFRQMMTAGAVSHAQPSIIKVGG
                     ITEFLKVAAIADELGVRIIPHSPYFGPGFLATLQLLSTRDEGLIEVFYMKRAACLWGG
                     RIAVDASGFVDVPSGPGLGYEPDKAVLEQFRAS"
     gene            complement(2756..3985)
                     /locus_tag="VM910_37870"
     CDS             complement(2756..3985)
                     /locus_tag="VM910_37870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68306.1"
                     /translation="MHSPRLSNVKITALAALLAATLASSAQAQKRYDPGATDTEIKIG
                     NIMPYSGPASAYATIGKTEAAYFNKINAEGGINGRKIDFISYDDAYSPPKTVEQARKL
                     VESDEVLLIFNPLGTPGNTAIQKYMNAKKVPQLFVSTGAAKWNDPKNFPWTMGWQPNY
                     QTEARIYASYILKNHPGKTIGVLYQNDDFGKDYLTGLREGLGDQASKLIVIETSYETS
                     TPTVDSQVVQIKGANPDIFVNITTPKFAAQAIKKIAELNWTPIHFLTNVSVSIGGVMK
                     PAGFENDQGILSTAYLKDPKDPDWKNDPAMKEWRTFMTKWYPEGDQDDASTVFAYGVA
                     KGLEQVLRQCGDDLTRENVMRQAANLNFEIGIYLPGMRIKTGPTDFAPLEQLQMMRFK
                     GETWERFGPMMSGEKGS"
     gene            4248..5081
                     /locus_tag="VM910_37875"
     CDS             4248..5081
                     /locus_tag="VM910_37875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI68307.1"
                     /translation="MNPAQRALWYIESHLADALTLDDISAIGGVSRFHMVRAFAAATG
                     FSVMRYVRARRLTEAARALAAGAPDILTLALDADYGSHEAFTRAFRDHFGVTPEAVRA
                     TTCLDRLKLQEPILMISTALDTLAPPRFEKSTAKLIAGVSDRFSHENGAGIPLLWQRF
                     HQTIGNIPNPVGRIAYGVCCNGDDAGNFDYIAGVEVRDFTDVPREFSRVRIPEARYAV
                     FTHTDHISTIRRTVNTIWNQWLPASGLKMADAPNFERYDEKFDPATGNGGFEIWIPVT
                     D"
     gene            5270..6199
                     /locus_tag="VM910_37880"
     CDS             5270..6199
                     /locus_tag="VM910_37880"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI68308.1"
                     /translation="MSEVRVLATDLEFPEGPVVMPDGSVLLVEIRGQRLTRIHPDGRK
                     EVVAKIPGGPNGAAIGPDGKCYICNNGGFSWVPTRNMLMPGPPAPHEYIGGSIQRVDL
                     QSGRIETVVDKCGDHPLNGPNDLVFDRHGGLWLSDLGKRRARDMDVGAFYYIKPGMKE
                     IVEGVFGLMPANGIGLSPDENTVYIAETPTARLWAFDLSAPGTVRPRDVIYRGERGKP
                     IAGLGGYQMFDSLAVEASGNVCVATLISGCISVIAPDGTIVEQVPTGDRVTTNIGFGG
                     PELKTAYITLSGKGELIAMQWSRPGLPLNFLNR"
     gene            6251..>6468
                     /locus_tag="VM910_37885"
     CDS             6251..>6468
                     /locus_tag="VM910_37885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVI68309.1"
                     /translation="MPWPEPVALHGEYARLEPLSRAHQNGLVEAVRDGELWQLWYTSV
                     PTPENMAKEIDRRLGLQAAGSMLPFTVF"
BASE COUNT         1163 a         1993 c         1987 g         1325 t
ORIGIN      
        1 attccagatc gtgatcatga tgaagggctg ggcgcgcttc atgtacgaag acaagccgac
       61 cctggtgtcg gccggcgacg tcgtgcatca gcggccgggg atcgtgcact acctgttcga
      121 ctactcgccg gacatggaat acctggaaat cgtcagcccg gcggacttca agaccgtcga
      181 catgccgccc gcgactgaca aggtgcctcc tgtcacgccc tggaagtgag gacgcgatgt
      241 cctcgcctcg ctccgccgcg ggttaaccct tgtcgtcggc cgccggttgc gagtctcgac
      301 ggccatgctg gatgtggtgc gccggatcaa gagggcgccg acatctagcc gtgaacaggt
      361 gagtacgacg cttgaacgct gattcggacg cggttcgttc gatcggcgtt ccgaagctta
      421 aagcgcgtcc gctttccgtc gcattttggc acagccgctg ctgatgggat tctctgtctg
      481 cgaactcgat ggccaggatg acggacttct gaaccgccac accagagcgc gatagcctta
      541 tgtcgaatcg ccgtcgcgct ctggtttatt attcgagcat gatctccgcg tgtgtcgcga
      601 gaaaaaccgg tcccgctttt ccggatcatg ctctcgtgta gtgtcgtcgc ggcatcctcc
      661 agccgacaac tgtcgcttcc agcgcaccgc cggactcgta gttgtgccat tgaccgtcga
      721 catagtggca agcaaatggg agcagatagg ttccgctctg atcctcgcac agcacctgga
      781 ccgccgtgcc aggtggcggt ttcccggcac catcgaattc agccagtcgt cgttcgcgcg
      841 tagccatagc tcgttccccg ctctctcgcg gcgcggcgcg ccgcctgcgt ttcccctgcc
      901 cgctctgccg tgccgcgtgt atccttttct tgggaaatct tttcttgaaa accacaacgc
      961 cggattgggg tatcaatgtg gtattcacgg cgtgccggac ctaagcaatt tgctgtgaag
     1021 agcgcgactt ttgtttgacg ggctccctca cgtgcgccgg taccgtgttg cttgagaacg
     1081 ctgtgtttaa gacgaggttt tgatgaagct gaggatttct tttgccgcga ttgcggcatt
     1141 gactgccccg atgctgatgc aagcggaact tcgagcccag gatattcccg gcatcgagat
     1201 ctgcacggtc gaaaagacca tggagcggcg cacatcgtgc ctgcagagca atgtcgaatt
     1261 cctgcagagg acagtcacaa agctcgcgtc cgattatcaa ttaaagctgg atgccgccaa
     1321 ccgccagatc gaagcgttga aagctgccgc cgtcgcgctg cagaaaacgg tggccgatct
     1381 ccagacgtcg cagaagcaga tgaccgacga cttgaagaag aaagccgatg cttcgccggc
     1441 aaaggacgcc gcgccgacga aacaaggcaa gtagccaccg gatttcgtta cgacgcgcgg
     1501 aattgctcca gcacggcctt gtccggctcg tagccgagcc cgggcccgga tggcacgtcg
     1561 acaaaaccac tggcatcgac ggcgatgcgg ccaccccaga ggcacgcggc ccgcttcatg
     1621 taaaagactt cgatgagccc ttcgtccctt gtcgacagca gttgcaacgt cgcaagaaag
     1681 cccggaccga aatagggcga atgcggaatg atcctgacgc cgagctcgtc ggcgatcgct
     1741 gcaaccttca ggaattcggt gatgccgccg accttgatga tcgaaggctg cgcgtggctc
     1801 accgcacccg ccgtcatcat ctggcggaat tgatacgccg tgcaggcatt ctcgccggcc
     1861 gcaatatcga gcccgccctt tgcgcgcacc tcggccagcg tgacgaaatc ttcgggcggc
     1921 cagaccggct cttccaggaa catcgggtct gcggcgcggc aggcctgcgc gaaggcgatc
     1981 gcggcggtgc cgtcgagcgg gcagttcatg tcgaccatca gcggcacgcc gtctccgatc
     2041 gccttgcgcg ccgcaaatac cgcgggaagc gtgatctcgt gcagcttgat ggcgccgtag
     2101 ccggctcgtc gcgccgcctc gcattcgccc gcgatgctct gctcgttccc gatccgcagc
     2161 aggctcgcat aggcgggaat tttgaggcgc ctggcgccgc cgatcaattg atgcagcggc
     2221 acgcccttca ccctggccgc gatatcccac agcgcgatgt cgagccccga aatggcaaac
     2281 atcgtgatgc cgtagcggcc gaacagatgc aggttgcgct ggatctcttc catgaaggcg
     2341 gggattttgt ccgcttccgg aacttcgcga ccacgcacct gtggcgcgat catctcgtcg
     2401 atggcggtgc aggtggttcg cggacagaca tagctgaagg catcgcccca gccggtgacg
     2461 ccttgatccg tcgagacctc gacaacaacc atgtcgagtt ccgagagtcc ggaagtgccg
     2521 tgccgaaatt tggcaacgcc gacatcatag gggatgcgga ggtgatgcgc tcggacgttg
     2581 gtgatctgca tgggagtacc tttctgtttc acccgcgcgc accgtcattg cggggagcgt
     2641 gagctccgaa gcaatccggc cttctggagt tggctccgga ctacttcgca gagcctgtca
     2701 tcgggccgcg cttcgcgccg acctgtcgtt ttgcaatgac gaaggaggcc gttcgtcaag
     2761 aacccttctc acccgacatc atcggtccaa agcgctccca cgtttcgccc ttgaagcgca
     2821 tcatctggag ctgttcgagc ggcgcgaaat cggtcgggcc ggtcttgatc cgcatacccg
     2881 gcaaatagat gccgatctcg aaattgaggt tcgcagcttg cctcatgacg ttctcgcgcg
     2941 tgagatcgtc gccgcattgc ctcaagacct gttccagccc cttggcgacg ccataggcga
     3001 acacggtgga agcgtcgtcc tgatcacctt ccgggtacca tttggtcatg aacgtgcgcc
     3061 attccttcat cgccggatcg ttcttccaat cggggtcctt ggggtccttc aggtaggcgg
     3121 tcgagaggat gccctggtca ttctcgaagc cggcaggctt catcaccccg ccgatcgaca
     3181 ccgacacgtt ggtgagaaag tggatcggag tccagttgag ttcggcgatc ttcttgattg
     3241 cctgggctgc gaatttcggc gtcgtgatgt tgacgaagat gtccgggttt gcgcccttga
     3301 tctgcaccac ttgcgaatcc accgtcggcg tcgacgtctc ataggacgtc tcgatcacga
     3361 tcagcttgct cgcctgatcg ccgagacctt cgcgcaagcc ggtgagatag tccttgccga
     3421 aatcgtcgtt ctggtagagc acgccgatcg tcttgccggg atggttcttc aggatgtagc
     3481 tcgcgtagat gcgcgcttcg gtctggtagt ttggctgcca gcccatggtc cagggaaaat
     3541 tcttcgggtc attccacttt gcggcgcccg tcgaaacgaa gagctgcggc actttcttcg
     3601 cgttcatgta tttctggatc gcggtgttgc cgggcgtgcc gagcgggttg aagatcagga
     3661 gtacctcgtc ggactccacg agcttgcgtg cctgttccac ggtcttcggc ggcgagtagg
     3721 cgtcgtcgta ggagatgaag tcgatcttgc gcccgttgat gccgccttcg gcgttgatct
     3781 tgttgaaata ggcggcctcg gtcttgccga tggtcgcata ggccgaggcc ggtcccgaat
     3841 agggcatgat gttgccgatc ttgatctcgg tgtcggtcgc gcccggatcg tatctcttct
     3901 gcgcctgcgc actgcttgcg agcgtcgccg caagcagcgc ggcaagtgct gttatcttca
     3961 cgttgctcaa gcgggggcta tgcatcgtcg ttctccctga cgtgttcccc gacggtcgcg
     4021 gcggtcttga tatctggcca gctctgtcag ccgtcggatc aatgcgccgc cgagtatgca
     4081 cgaacgggag gatgtgacca gcaacggtgt cgctgccatt ttccgtcgcg tggaagtaga
     4141 attggcaagt tcccgtctct ctgcgagcgg atgagcgcaa ggactatcaa acgccgatgc
     4201 ggcagaacgc gcaggatcgg cgtgcttcga acgaaggcct cacatccatg aatccggccc
     4261 aaagggcgct ctggtacatc gaaagccatc tcgccgatgc gttgaccctc gacgacattt
     4321 cggcgatcgg cggcgtctcg cgctttcaca tggtgcgggc gtttgccgcc gccaccggct
     4381 tctcggtgat gcgttacgtg cgtgcccggc gcctcaccga ggcggcgcgt gcgctcgcgg
     4441 ccggcgcgcc cgatattctg actctggcgc tggacgcgga ttacggctct cacgaagcct
     4501 tcacccgcgc gtttcgcgat catttcgggg tcacgcccga agcggttcgc gccacgacat
     4561 gcctcgaccg cctcaagcta caggagccga tcctcatgat ttccaccgca ctcgacactc
     4621 ttgcgccccc gcgtttcgaa aagagcaccg ccaaattgat cgccggggtc agcgatcgct
     4681 tcagccatga gaatggcgcc ggtattcccc tgctttggca acgctttcac caaacgatcg
     4741 ggaacatccc gaatcccgtt gggcggatcg cttacggggt ctgctgcaat ggcgacgatg
     4801 ccggaaactt cgattacatc gccggcgtcg aagtgcgcga cttcaccgac gtgccgcgcg
     4861 agttcagccg cgtccgcatt cccgaggcgc gttacgccgt gttcacccac accgaccaca
     4921 tctcgaccat ccgccgcacc gtcaacacca tctggaatca gtggctgccg gcctccggcc
     4981 tgaagatggc ggacgcgccg aacttcgagc gctatgacga gaagttcgat cccgcgaccg
     5041 gcaatggcgg cttcgaaatc tggatacccg tgacggacta ggttcctcgc ctcagcgaag
     5101 agttgccgac atcgggcctg ccggcttgcc gcaacgctgc cttgcagcgg ccggcgcttg
     5161 cttggcaagc gggggcgact ttgccataac ggaacgcaac aacatgttgc gtgcgcggga
     5221 cgtgctttgg aaaccgcctg cagctcacca gaaagcggga ggttttccga tgtccgaggt
     5281 ccgcgtgctc gccactgatc tggagtttcc ggaaggaccg gtggtgatgc cggacggatc
     5341 tgtcctgctg gtcgagattc gcggccagag gctgacgcgc atccaccctg atgggcgcaa
     5401 ggaagtcgtg gcaaaaattc cgggcggccc caacggagcc gcgatcgggc ccgacggcaa
     5461 atgctacatc tgcaacaatg gcggttttag ctgggtcccg acgcgcaaca tgctgatgcc
     5521 gggaccgccc gcgccgcacg aatatatcgg cggctcgatt cagcgggtcg atctgcagtc
     5581 aggcaggatc gagaccgtcg tcgacaagtg cggcgatcat ccgctgaatg ggccgaacga
     5641 tctcgtgttc gaccggcatg gcggcctgtg gctctccgac ctcggcaagc ggcgggcgcg
     5701 cgacatggat gtcggcgcct tctactacat caagcctggc atgaaggaga tcgtcgaagg
     5761 tgtgttcggc ctgatgcctg ccaacggcat cgggctgtcg ccggacgaaa acactgtcta
     5821 catcgcggag acgccgacgg cgcgcttgtg ggccttcgac ctgtcggcgc ccggcacggt
     5881 ccgcccgcgc gacgtgatct atcgcggcga gcgcggcaag ccgatcgccg ggctcggcgg
     5941 ttaccagatg ttcgattcgc tggcggtcga agcctcgggc aatgtctgcg tcgccacctt
     6001 gatctccggc tgtatctcgg tgattgcgcc ggatggtacg atcgtcgagc aggtgccgac
     6061 gggagatcgc gtcaccacca acattggctt cggcgggccg gaattgaaaa ctgcctacat
     6121 caccctgtcc ggcaagggcg agttgatcgc gatgcaatgg tcgcggccgg gtttgccgct
     6181 gaattttctg aacaggtgat gcctttgtcg cttgcgctcc gcgcaatgac ggagcgggaa
     6241 ggagtctccg atgccttggc ctgaacctgt cgccttgcac ggtgaatacg cacggctcga
     6301 gccactgtca cgggcgcatc aaaatgggct cgtcgaggcc gtgcgcgacg gtgagctctg
     6361 gcaactctgg tacacctctg tgccaacacc tgagaacatg gccaaggaaa tcgatcgtcg
     6421 cctcggcttg caggcggcgg gatcgatgct gccgttcacc gtgttcga
//
LOCUS       DATLJT010001665         2937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2012439,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001665 DATLJT010000000
VERSION     DATLJT010001665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012439"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="tolB"
                     /locus_tag="VM910_37890"
     CDS             complement(<1..785)
                     /gene="tolB"
                     /locus_tag="VM910_37890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564420.1"
                     /note="forms dimers; may be involved in cell envelope
                     integrity; interacts with outer membrane proteins and with
                     the C-terminal domain of inner membrane protein TolA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HVI68310.1"
                     /translation="MPHDRPVFPLTRRTLTLGALAGGLALLPRRARAAVRLDITEGNF
                     QPLPIAIPKFFAAGQDDGDAANAVTQIITANLQHSGLFAPIDPTAYIERIASVDAVPR
                     FPDWRNINAQALVTGRIGRQADGRLQAEFRLWDVLGGQQLDGKQYITTPDNWRRIAHI
                     ISDAIYERLTGEGGYFDSRVVFVDETGPPERRVKRLAIMDQDGANVRYLTRGDELVLT
                     PRFSPSTQEITYMAYGQGDPRVYLYNIETSQREIVGNFPGMSFS"
     gene            complement(806..1738)
                     /locus_tag="VM910_37895"
     CDS             complement(806..1738)
                     /locus_tag="VM910_37895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68311.1"
                     /translation="MTISCVGHAAALIFGLMAISAQPMEAPPVQALPVSFVSEKDFSQ
                     LPLGVKNAPQLKVPDIKPLADKVDEQKSVDQQAPKVSAKPDITTPAAKPVSEAKPEPK
                     PEPKPEPKPELKTELKPAPKPADKPDKPKPPEYRPDQIANLLKKDAAKDPPKQQDAPA
                     QPARDTPKFNADQVAQLLDKREPQRQVATAQSLNDSPTLGAAIGAQNAQLSQSEIDAL
                     RARISSCWSPPPGIDANSRVYVVLRVLFKPDGSMVQAPTLVEGSASALGPALAESAKR
                     ALLLCQPFTMLRPQHYDQWKDLELKFDPHELLGG"
     gene            complement(1747..2190)
                     /gene="tolR"
                     /locus_tag="VM910_37900"
     CDS             complement(1747..2190)
                     /gene="tolR"
                     /locus_tag="VM910_37900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HVI68312.1"
                     /translation="MNIAAAGGRGKRRRRPVMSEINVTPFVDVMLVLLIVFMVSAPLL
                     TVGVPIDLPQSQAKALEQNNEPLTVSVNNQSQIFLQNDEIKIDDLLPKLKAIVDARGG
                     TSNDLIYIRGDRTVAYGMMMRVMGRISGAGYHRIALVTEVEQGSQ"
     gene            complement(2215..2928)
                     /gene="tolQ"
                     /locus_tag="VM910_37905"
     CDS             complement(2215..2928)
                     /gene="tolQ"
                     /locus_tag="VM910_37905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HVI68313.1"
                     /translation="MNPADISSSVAPLTSSNLSLFHLFWHAHWIVESVMAGLLVCSVW
                     VWAIAIDKTLLFARLRRAGDHFEEAFWSGQSLEDLYKSLATQPGHSTGALFVAAMREW
                     KRSLEGHVRSLAGLQMRIEKVMDVAIAREVEHMERRLLVLATVGSAGPFIGLFGTVAG
                     IMTSFQSIAASQNTSLAVVAPGIAEALFATAIGLVAAIPATIFYNKFSSEVNRQAQRL
                     EGFADEFTAILSRQIDEGG"
BASE COUNT          549 a          891 c          912 g          585 t
ORIGIN      
        1 gagaagctca tgccgggaaa gttgccgacg atttcacgct ggctggtttc gatattgtac
       61 aaatagacgc gcggatcgcc ctggccgtag gccatgtagg tgatttcctg cgtcgatggc
      121 gagaaccgcg gggtcagcac gagctcgtcg ccgcgggtga gatagcgcac attggcgccg
      181 tcctgatcca tgatcgcaag ccgcttcacg cgccgctccg gcggaccggt ctcgtcgacg
      241 aacaccacgc ggctgtcgaa atagccgccc tcgccggtga gccgttcgta aattgcgtca
      301 gagatgatgt gggcgatgcg ccgccaattg tctggcgtgg tgatgtattg cttgccgtcg
      361 agctgttgtc cgccgagcac gtcccacagc cggaattccg cctgcaggcg accgtcagcc
      421 tgccgcccga tgcggccggt gacgagtgcc tgggcgttga tgttgcgcca atcaggaaaa
      481 cgcggcaccg catcgacgct cgcgatccgc tcgatatagg cggtcgggtc gatgggagcg
      541 aacaggccgc tgtgttgcag gttcgccgtg atgatttgcg tgaccgcgtt ggcggcgtcg
      601 ccgtcgtcct gcccggcagc gaagaatttc ggaatcgcaa ttggcagcgg ctggaaattt
      661 ccctcggtaa tgtcgagacg aacagcggcg cgcgcgcggc gcggcagcag cgccaagcca
      721 ccagcaagcg cgcccaaggt aagcgtgcga cgcgtcaacg gaaaaacagg tctatcgtga
      781 ggcatgacaa ctcttgtaag cgtgatcagc cgcccagcag ttcgtgtgga tcgaatttaa
      841 gttccagatc tttccattga tcgtagtgtt gaggcctaag catcgtgaat ggctgacaca
      901 atagtaaggc gcgcttggcg ctttccgcga gcgccggtcc aagcgccgag gcggagcctt
      961 cgaccaaagt cggcgcctgc accattgagc cgtcgggttt gaacagaacg cggagcacga
     1021 catagaccct ggaattggcg tcgatgcctg gaggcggact ccagcagctg gaaatgcggg
     1081 cacgcaaagc atcgatctcg ctctgcgaca gttgtgcgtt ttgcgccccg atcgccgcgc
     1141 caagggtcgg cgaatcgttg agcgactggg cagtggcgac ttgccgctgc ggctcgcgct
     1201 tgtcgagcaa ctgggcgacc tggtccgcgt tgaatttcgg cgtgtcgcgc gcgggctgcg
     1261 ccggcgcgtc ttgttgcttg ggcggatcct tggccgcatc tttctttaag agattggcga
     1321 tttgatccgg ccgatattcc ggcggtttcg gtttatctgg tttgtccgcc ggtttcggcg
     1381 ccggtttgag ttctgttttg agctcgggct tgggctcagg ctttggctcg ggcttcggtt
     1441 caggctttgc ttccgaaacc ggtttcgccg caggcgttgt gatgtccggc ttggcggaaa
     1501 ccttcggcgc ctgctgatcg accgatttct gctcgtcgac cttgtcggcg agcggcttga
     1561 tgtccggaac cttcaactgc ggcgcattct tcacgcccaa gggcagctgg ctgaagtcct
     1621 tttcggagac gaaggaaacc ggcagcgcct gcaccggcgg cgcctccatc ggttgggccg
     1681 agatcgccat gaggccgaag atcagcgcag cggcgtgacc gacgcacgat atggtcatgc
     1741 cgggcttcat tggctgccct gctcgacttc ggtgaccagc gcgatgcgat gataacccgc
     1801 gcccgaaatg cggcccatca cccgcatcat catgccataa gcgacggtcc tgtccccgcg
     1861 aatatagatg agatcgttgc tggtcccgcc gcgggcgtca acgatcgcct tgagcttggg
     1921 caacagatcg tcgatcttga tctcatcgtt ttgcaggaag atttgactct ggttgttgac
     1981 cgaaaccgtc aacggctcgt tgttctgttc cagcgccttg gcttgactct gcggcaggtc
     2041 gatcggcacg ccgacggtca gaagcggcgc cgacaccatg aaaacaatca acagcaccaa
     2101 catgacgtca acgaacggcg tcacattgat ttcgctcatg acgggtcgcc gccggcgctt
     2161 acctcttccg ccggctgcgg caatgttcat gctgcccgcc gccatatcct gccctcaccc
     2221 accctcgtcg atctgtcgcg acaggatcgc ggtgaattcg tcggcgaagc cttcgagccg
     2281 ctgcgcctgt cggttcacct cgcttgagaa tttattataa aaaatggtcg ccgggatggc
     2341 ggcaacgagg ccgattgcgg tggcaaacaa agcctccgcg atgcccggcg cgaccactgc
     2401 gagcgaagta ttctgcgacg ccgcgatcga ttgaaagctc gtcatgatgc cagccacggt
     2461 gccgaacagg ccgataaaag gtcctgccga accgaccgtc gccagcacca gcaagcgccg
     2521 ttccatatgt tcgacctcgc gggcgatggc gacgtccatg accttctcaa tgcgcatctg
     2581 cagtcccgcc aacgagcgga cgtggccttc gaggctgcgc ttccactcgc gcatggcggc
     2641 aacaaacagc gcgccggtcg aatgccccgg ctgtgtcgcc agcgacttgt acagatcttc
     2701 cagcgattgg cccgaccaga acgcttcctc aaaatggtcg ccggcgcgcc gcaaccgcgc
     2761 gaacaacagc gtcttgtcga tggcgatcgc ccacacccag accgagcaaa cgagcaggcc
     2821 cgccatcacc gattccacga tccaatgcgc gtgccagaac agatggaaca gcgaaagatt
     2881 ggaggatgtc agcggtgcga ccgacgagga aatgtcggca ggattcatag agcgtcc
//
LOCUS       DATLJT010001666         3403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1783805,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001666 DATLJT010000000
VERSION     DATLJT010001666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783805"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(526..1791)
                     /locus_tag="VM910_37910"
     CDS             complement(526..1791)
                     /locus_tag="VM910_37910"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI68314.1"
                     /translation="MIFLTDTIVRNLQPPAKGAKIHRDADAPGAPGTGVTGFGIRITA
                     AGFRAFVLEYRSRETGDQRVFTIGRFPTIKTERAREKARNLRAEIEDGGDPQAERVAK
                     RHEATVHDLADRFEREHLPLKKPSTAESYSRLLRLYIRPHLGRMRISTVTQADTERLC
                     RAVTEAAGPYQANRVQAVGSKLFTSALRWQLRTEVHGNPFKRVERHKEHARRRYASAE
                     ELRRLVAALAKHPDRQGADAIKLLLLTGARRGEVLSMRWGDLDLTAGTWNRKAADLKQ
                     GRDHSVPLSPPALTILSALRDEQTANGKRALGEFVFPSAVSTARHLVEVRRLWRNVVK
                     TADLGDLRLHDLRHSFASQLVSSGASLALIGSLLGHSSPAVTARYSHLFDSVERDAVT
                     RVGAIIEAAGLEHPPSAPVIPLSDQRRKK"
     gene            2028..3166
                     /locus_tag="VM910_37915"
     CDS             join(2028..2475,2475..3166)
                     /locus_tag="VM910_37915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611739.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI68315.1"
                     /translation="MAVKKYVVRLSAEERAQLDELIRKGKRSAQLLTKARILLKADVS
                     ERGEGWSDSRIAAALDTSIATIERTRRQLVEEGFEAVLRRKYNPNSARPRIFDGAAEA
                     KLIALTLSPAPEGFARWSLRLLEEKVVELHIVERASDNTIGRTLKKTFLKPHRKQQWV
                     IPPDANAAFVATMEDVLEVYQRPHDPQRPLVCLDETSKQLIIDTRAPIPAKPGQPARH
                     DYEYERNGVANLFMVFAPLEGWRHVKVTDRHTAIDYAHVLRDLSDTHFPDAAKIVLVQ
                     DNLNIHKPASLYEAFPAPEARRLVERFEWHYTPKHGSWLDMAESELGVLASQCLDRRI
                     ADKQILAHEVTAWQDHRNKHHAKADWQFTTANARVKLKRLYPQFE"
     gene            3253..3403
                     /locus_tag="VM910_37920"
                     /pseudo
     CDS             3253..3403
                     /locus_tag="VM910_37920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT          672 a         1042 c         1052 g          637 t
ORIGIN      
        1 gcctcgttcg ggggggcgaa tccgattcgg aagcacggta acgtcgcaaa tatcgactct
       61 ggttatttct tgaccagatg acaggattgg tctttccttc atggcgacct ccgaactcgc
      121 cgtcgcttgg gcttgctgac cgcatcagcg atgacctgac gcgtcgcgcg gtctgggacg
      181 taggcgacgc ccccgaagat cgtcgtgggc aacgaaggcg aacgagggtg cgctcgcaca
      241 caccgagcag cgcggcttgc tgcttacgcg gcacgaggcg gtcaccattc gggagcgcga
      301 taggcggccg cacctcgctc ttgcgcggac ggccgcgttt ggctttgggc gctggaatct
      361 cgacgatagg gagaggggtc tcaggcacgg ttgcacctct tctcggtgct ccggcaaaca
      421 aaaaaagccg caggagcaac cagtgtagca aatctggtta ctattacggc gtataatgtt
      481 atgaagcgcg cctatactat gacacacctt cgcgcaaccg gtttattatt tttttcgtcg
      541 ctgatcagac agtggaataa ccggcgcgga cggcggatgc tcgagtccgg cagcttctat
      601 gattgcgccc acacgggtca cggcatcgcg ctcgacgctg tcgaatagat gactgtagcg
      661 cgccgtcacc gccggactgg agtgcccgag cagactaccg atgagcgcca acgacgcgcc
      721 gcttgaaaca agctgagatg caaatgaatg tcgcagatcg tgaagtctca gatcgccgag
      781 atcggccgtc ttcaccacgt ttcgccatag ccgacggacc tcaacgagat gtctggccgt
      841 actgacggcg cttggaaaga cgaactcacc gagcgcgcgc ttcccgttgg cggtctgttc
      901 gtcacgaagc gccgacagga tcgttagcgc gggcggtgat agcggcaccg agtgatcgcg
      961 gccctgcttt aggtctgccg ccttccgatt ccacgtccca gccgtgagat cgagatcgcc
     1021 ccaacgcatc gacaggacct cgcctcttcg tgcgccggtg agcagcaata gcttgatcgc
     1081 gtcggccccc tggcgatcgg ggtgcttcgc cagcgccgcc accagtcggc gcagttcttc
     1141 agccgaagca tagcgtcgcc gcgcgtgctc tttgtgccgc tcgacacgtt tgaacgggtt
     1201 gccatggacc tcggtgcgca attgccaacg tagcgcgctg gtgaacagct tcgagccgac
     1261 ggcctggacg cggttcgcct gatacggccc ggcggcttcg gtgacggcgc ggcacagccg
     1321 ttcggtgtcg gcctgggtga ccgtgctgat gcgcatgcgg ccaaggtgcg gccggatgta
     1381 gagcctcagg aggcgcgagt agctctccgc ggtgctcggc ttcttcagcg gcaggtgttc
     1441 gcgctcgaag cgatcggcga ggtcgtggac ggtggcctca tggcgcttgg cgacacgttc
     1501 ggcctgcgga tcgcccccgt cctcgatctc ggcgcgcaga ttccgcgcct tctctcgagc
     1561 ccgctcggtc ttgatggtcg gaaaccggcc gatggtgaac acacgctggt cgcccgtctc
     1621 ccggctccgg tattcgagga cgaaggcgcg gaatccggcg gcggtgatgc ggatgccgaa
     1681 gccggtgacg ccggtgccgg gggcgccagg agcgtcggcg tcgcggtgta tcttcgcgcc
     1741 cttcgcaggc ggctgcaggt tgcgaacgat ggtgtcggtg aggaagatca tggaagctcc
     1801 gtatcgtcgc catatcgtca gtaacgacga catgcgctgt ccaacttgtg tcacgcggtg
     1861 tcgttcgttg tccaggaaaa atacaggctt tatcggaggt tacggcagca gctcgtggac
     1921 aatcaagcac ttagcgggag cccctgctca aaattgaaaa tgacggcaac ttctgaatct
     1981 ctgtcgctgc aaggcagcga tggaggtgat tcgtggtcgg gaaagagatt gccgtcaaga
     2041 agtacgtagt gcggttaagc gctgaggaac gcgcccaact cgacgagttg atccgcaaag
     2101 gcaagcgttc cgcccagctg ctgacgaagg cccgcatcct gctaaaggca gacgtatcgg
     2161 aacgcggtga aggctggagc gacagccgga ttgccgcggc gttggacacc agcatcgcca
     2221 ctatcgagcg gaccaggcgc cagctggtcg aggaggggtt cgaggcggtg ttaaggcgca
     2281 aatacaatcc caattccgcg cggccgcgga ttttcgacgg cgcagcagaa gcaaaattga
     2341 tcgcgctgac cctctctccg gccccggagg ggttcgcccg ttggagtctg cgcctgctcg
     2401 aggagaaggt cgtcgaactg cacattgtcg agcgcgccag cgacaacacg atcgggcgga
     2461 cgctaaaaaa aacattctca agccgcatcg caagcagcaa tgggtgattc cgcccgacgc
     2521 caacgcggcg tttgtcgcca ccatggaaga cgtcctggaa gtctatcagc gaccgcacga
     2581 tccgcagcgc ccgctcgtgt gcctcgacga aacctcgaag cagctgatca tcgacacgcg
     2641 cgcgccgatc cccgccaaac ctggacaacc agcgcgtcac gattacgaat acgaacgcaa
     2701 tggcgtcgcc aatctgttca tggtgttcgc gccgctggaa ggctggcggc atgtcaaggt
     2761 caccgaccgc cacaccgcca tcgattatgc ccacgtgctc agggatttgt ccgatacgca
     2821 ttttccagat gcggcgaaaa tcgtgctggt gcaggataat ctcaacatcc acaagccagc
     2881 ctcgctttat gaggcctttc ccgcgcccga ggcccgccgg ctcgtcgagc gcttcgaatg
     2941 gcactacacg cccaagcacg gcagctggct cgacatggcc gaatccgaac tcggcgttct
     3001 cgcgtcgcaa tgtctggatc gccgcatcgc cgacaagcaa atcctcgccc acgaagtcac
     3061 cgcatggcag gaccaccgca acaagcacca cgccaaagcc gactggcaat tcacaaccgc
     3121 caacgcccgc gtcaagctga agagactgta ccctcagttc gagtgactcg ggcgactagc
     3181 caactgctgg ctctggaccg gtatgaacgc cgggcgctat ctcgacgtaa atttgccatt
     3241 cgagcatttg atttggcgaa gcgcggcttc gcgggaccga tcttttgctc ccgtgccact
     3301 gccaatctgt gcgcgatcct gctgcctgac gccgagcatt tgcaggaaaa ggatgccgaa
     3361 tatgctaacc gtcatggatt ctcgaagcac aagcctgcat tgc
//
LOCUS       DATLJT010001667         4701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2930545,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001667 DATLJT010000000
VERSION     DATLJT010001667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2930545"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VM910_37925"
     CDS             complement(<1..1050)
                     /locus_tag="VM910_37925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68316.1"
                     /translation="MSDGRKTKFKEPMPDVELPKMKLEVESEAKPETPLTAEQIYLKR
                     KVEVAQQDNEDFSNYLREKAARDQQQEPPSAPTPQTAPRKLNRRSLLTWAAGTAVVGE
                     AVTLGYPVLTGQSIPDHGARHAGATYTEPQRHPIDRELVNPQADVGGRRFGDWVLLMP
                     TKMSGGTYAVNLNTGRALAWISYWNYGDYNPISHHLCAFPSADPSKGFEWINSTQGGK
                     NSLIYGIPTQIETPGEGFNIYRVRYDGSQMEVMENVAETTGLGLGVHVNIDPKTAEHY
                     FVTDGQKDIAACFDRRTSRVIAALKYDWVPNVRNLAEAWQKGGVLKISKIYPDPATGK
                     YDYLGTKGQKIEWEMV"
     gene            complement(1164..2117)
                     /locus_tag="VM910_37930"
     CDS             complement(1164..2117)
                     /locus_tag="VM910_37930"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVI68317.1"
                     /translation="MTVITGFLGSGKTTLVKHILSNQQGLRTAVIVNEFGDIGIDSEL
                     IVAADDGMVELENGCICCSINSNFVDAIFRILNRDRKLDYLVVETTGLADPLPVVLTF
                     LRSELRTSMRVDSIVTVADAASFCLELFESEAARNQLVYGDMILLNKRDLVDAERLQA
                     LEATIRAVKAGARIVRTVQCQVPLPLILDVGLFQFDEFFAAKEHSAHRHETAHGNAHD
                     HVTADGFESLSFQSEQPFAVHKFQDFLERLPDNVYRAKGLLWIDESKKRYVFHLVARR
                     FSLDESPRDGPMKNKLVLIGRHLDCERLRDQLQACLAPPAR"
     gene            complement(2270..3286)
                     /locus_tag="VM910_37935"
     CDS             complement(2270..3286)
                     /locus_tag="VM910_37935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68318.1"
                     /translation="MPEVSTPAPPLKAVDSLVVEVITDDVSDAYVSKTLFAVSEFSNV
                     ILAGAKVISGETLLCANLGLGLRLVSEADGVRHTLMFDTGPEGTIFIRNCANLGIRMG
                     EVECIAVSHGHWDHMAALPDAVDAIVKEGGRVTVHVNPGMFNERAVRLKGGTVVPVAN
                     VPLPVDLEKRGAHVVNRGDERLLLDDHFYLSGEIPRVTSFEKGRVDHLCRTRPDGPWE
                     PDPLLMDERMLVAHVRDLGLIVFSACSHAGIVNVCIHARDLFPGIPIYCVMGGLHLGG
                     VMERIIPETVDGLRPFRINHIITGHCTGWRALHALANAFGDAVSQSAVGTKYTFDAGH
                     HLSV"
     gene            3424..4236
                     /locus_tag="VM910_37940"
     CDS             3424..4236
                     /locus_tag="VM910_37940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI68319.1"
                     /translation="MDGIVERVGVFALVGLSSGFVSGLFGIGGGIVRIPLFVYLLPLF
                     GVPHPVMMHLAVGTSMALVLPSAIASTRKQLVLGNLDLKFFRTWALGILVGVLIGTAL
                     LPYASTEILQVIFALFMLTVGVYEGLLKDRLVIAPAAPQGAVKLAVAAAIGCLAALTG
                     TGGGTMTTPVLQAFSVRLEIAIATASATGLVTGTIATIGAVVGGWHTPNLPAYSLGYV
                     DLAIFAAMLPTIMIAAPIGVRAGDRLSEAWLRRIYTALLFVIAFDLIRKLVS"
     gene            complement(4315..>4701)
                     /locus_tag="VM910_37945"
     CDS             complement(4315..>4701)
                     /locus_tag="VM910_37945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68320.1"
                     /translation="NVGGAEPIYVMAPDMRAYVQVGTVWQEVPLTPADDSATGVTKIE
                     GKQTYRYRFDARVRDFAQLLPNYMHVRFSGTMLVSPSSRPNGDVFERKDNYYVYLKPF
                     DVADEVVLKRMRFSGKPPVWIPMPPH"
BASE COUNT          849 a         1427 c         1451 g          974 t
ORIGIN      
        1 caccatctcc cattcgatct tctgcccctt ggtgccgagg tagtcgtatt tgccggtcgc
       61 cgggtcggga tagatcttgg agatcttgag cacgccgccc ttctgccaag cctcggccag
      121 gttgcgcacg ttgggcaccc aatcgtattt gagcgccgcg atcacgcgcg atgtccggcg
      181 gtcgaagcag gccgcgatgt ccttctgccc gtcggtgacg aaatagtgct ccgcagtctt
      241 gggatcgatg ttcacgtgca cgccgagacc gaggcccgtg gtttccgcga cattctccat
      301 cacctccatt tgcgagccgt cgtagcgcac gcgataaatg ttgaagcctt ccccgggcgt
      361 ctcgatctgg gtggggatgc cgtagatcag cgaattcttg ccgccctggg tgctgttgat
      421 ccattcgaag cccttcgacg gatccgcact cgggaaggcg cacaaatggt gcgagatcgg
      481 gttgtagtcg ccgtagttcc agtacgatat ccaggcgagt gcccggccgg tattgaggtt
      541 cactgcatag gtgccgccgc tcatcttggt cggcatcagc agcacccagt cgccgaagcg
      601 ccggccgccc acgtctgcct gcggattgac caattcgcga tcgatggggt ggcgctgggg
      661 ttcggtgtaa gtggcgccgg cgtgccttgc cccgtggtcc gggatgctct gcccggtgag
      721 cacggggtag ccgagcgtga ccgcctcgcc caccaccgcg gtgcccgccg cccaggtcag
      781 caggctgcgc cgattgagct tccgcggcgc ggtttgcggc gtcggtgccg atggcggttc
      841 ttgctgctga tcgcgggcgg ctttttcccg cagataattc gaaaagtctt cgttgtcctg
      901 ctgcgccacc tcgaccttgc gcttgaggta gatctgttcg gcggtgagcg gcgtctcggg
      961 cttggcctca ctttcgacct cgagcttcat tttcggaagc tcgacgtctg gcatcggctc
     1021 tttgaatttc gtcttgcgcc cgtcggacat ggcccagctc ctgttctctt gtgcagctcc
     1081 gcattatctc atcacgacgt tgctcaggcg atcaagatca tccagctctg gtcgaattcg
     1141 atcaaagcct caatggagcg ccgctagcgt gccggcgggg cgaggcaggc ctgcagctga
     1201 tcgcggagcc gctcgcaatc gagatgccga ccgatcagca cgagcttgtt tttcatgggg
     1261 ccatcgcggg ggctttcatc tagcgagaat cgtcttgcca ccaagtgaaa gacgtagcgc
     1321 tttttgctct cgtcgatcca aagcaggccc ttggcgcgat agacgttgtc cggaagccgc
     1381 tcgaggaaat cttggaactt gtggacggca aagggctgct cgctctggaa tgacagggac
     1441 tcgaaaccgt ccgccgtcac gtgatcgtga gcgttcccgt gtgccgtctc gtgacgatga
     1501 gcagaatgct ccttcgccgc aaaaaactca tcgaattgga acaggccgac atcaaggatc
     1561 aacgggagcg gcacctggca ctgcaccgtg cggacgatcc gcgctccggc cttgacggcc
     1621 cggatcgtgg cctcgagtgc ttgcagacgc tcggcgtcga cgagatcgcg cttgttgagc
     1681 aagatcatat cgccgtagac gagctgattg cgggcagcct cgctctcgaa cagttccagg
     1741 caaaagcttg cggcatccgc gaccgtgacg atcgaatcga cgcgcatcga agtccgcaat
     1801 tccgagcgca ggaaggtgag caccacgggc aaggggtcgg cgagaccggt ggtttcgacc
     1861 accaggtagt cgagcttgcg atcgcggttg aggattcgaa agatcgcatc gacgaagttg
     1921 ctgttgatcg agcagcagat gcagccgttt tccagctcca ccatgccgtc gtcggctgcg
     1981 acgatcagct cgctgtcaat gccgatgtcg ccgaactcgt tgacgatgac agcggtccgc
     2041 agcccttgct ggttgctcag gatgtgcttc acaagggtcg tttttccgct gccgaggaag
     2101 ccggtgatga cggtcaccgg caaccgggcc ctggacggag gcgctgcaaa agctggcggg
     2161 gaagcagtca tgcggaagcc gctttatcga cttttgtccc cgcgcgtgca ggggacctat
     2221 gttccagagg cttggttctt tgaccggacc gcagtccggc cctcgtttcc taaaccgaga
     2281 ggtggtgccc agcatcgaac gtatattttg tgccgaccgc agactgactg accgcgtcac
     2341 caaacgcatt ggcaagggcg tgcaacgccc gccaacctgt gcagtgaccg gtgatgatgt
     2401 gattgattcg aaacggccgc agcccgtcca ctgtctcggg gatgatccgt tccatgacac
     2461 cgcccaggtg aagcccaccc attacgcaat agatcggaat gcccggaaag agatcgcggg
     2521 catgaatgca cacgttgaca atgccagcgt gggagcaggc gctgaagacg atcaatccca
     2581 aatcacggac gtgcgcaacc agcatgcgct cgtccatcag cagcggatcg ggctcccagg
     2641 ggccgtcggg gcgcgttcga cacagatgat cgacgcggcc tttttcgaag gacgtcacgc
     2701 gcggaatttc accgctcaag tagaaatgat catcgagcag cagtcgttca tcaccgcgat
     2761 tgaccacgtg cgccccgcgc ttctctaagt cgaccggaag cggaacgttc gcgaccggca
     2821 cgaccgttcc gcccttgagc cgaaccgcgc gctcgttgaa cattccggga ttgacgtgga
     2881 cggtgacgcg cccgccctct ttcacaattg catcgaccgc gtccggcagc gccgccatgt
     2941 gatcccagtg tccatgactc acggcgatgc attcgacctc gcccattcga attccgagat
     3001 tggcgcagtt gcgaataaaa atggtgccct ccgggccggt atcgaacatg agggtgtgtc
     3061 gcaccccatc agcttcggat acaagccgca ggccaagtcc cagattcgca cagagaagcg
     3121 tctcgccgga aatcaccttc gcgccggcga gaatgacgtt ggaaaattca gatactgcaa
     3181 acaaggtctt gctaacatag gcgtcgctga cgtcgtccgt tatcacctct acaacgaggg
     3241 aatcgacggc tttgagaggg ggcgctggtg tgctgacttc aggcatttgc gcaggtttcg
     3301 atggcgagac acctacggac cgcctgcgag gctacccttt gctcccaagg cagacaacta
     3361 cgggtcctgc atttcaaatg ctcgcgcggg gaataacagt ggcatgacat ctgggaacgc
     3421 atcatggatg gaattgtgga gcgtgtcggc gtgttcgcgc tagtgggcct ctcgtccggc
     3481 ttcgtgtcgg ggctgttcgg catcggcggg ggcatcgtcc gaattcccct cttcgtttat
     3541 ctcttgccgc tgtttggagt gccgcatccg gtcatgatgc atctggcggt gggaacctcc
     3601 atggcactgg tactcccgag cgcgatagcg tcgacgcgca agcagctggt gctcggcaac
     3661 ttggatctca agttcttccg cacctgggcc ttggggattc tggtgggcgt cctgattgga
     3721 actgcgctgc tcccttacgc gtccactgag attctccagg tcatcttcgc gctgttcatg
     3781 ctcacggtcg gcgtctacga gggcttgctc aaggatcgcc tggtgatcgc gccggcggcg
     3841 ccgcagggtg cggtcaagct cgctgtggcc gcggcgatcg gctgcctggc ggccttgacc
     3901 ggaaccggcg ggggtacgat gacgacgccg gttctgcagg cgttcagcgt gcggttggag
     3961 attgccattg ccaccgcgtc ggcgaccgga ctagtgacgg gcaccatcgc caccatcggc
     4021 gccgttgtgg gaggttggca cacgccgaat ctgccggcgt attccctggg ctacgtcgac
     4081 ctcgccattt ttgccgcgat gctgccgacc atcatgatcg ctgcgccgat cggcgtgcgc
     4141 gcgggcgatc ggctgagcga ggcctggctg cgccgcatct acaccgcgct gctgttcgtg
     4201 atcgcgttcg atctgatccg aaaactggtg agctagtctt agccaccatg ccacaacaat
     4261 tcgtcgccga ggcgaatcgc gtcccgcccg ggtatccgca aaccatcggt aacctcagtg
     4321 cggcggcatg gggatccaga ccggcggttt tcctgaaaag cgcatacgtt tcaaaacgac
     4381 ctcatcggca acgtcgaacg gcttgagata gacgtagtaa ttatccttgc gctcgaacac
     4441 gtcgccgttc ggccggctgc tcggactgac cagcatcgtg ccggagaagc gcacgtgcat
     4501 atagttgggg agcagttgcg cgaaatcgcg cacgcgcgca tcgaaacgat agcgataggt
     4561 ctgcttgccc tcgattttcg tgacgccggt tgcgctatcg tcggccggcg tcagcggcac
     4621 ttcctgccag accgttccga cctgcacata ggcgcgcata tccggcgcca tgacatagat
     4681 cggctccgcg ccgccgacgt t
//
LOCUS       DATLJT010001668         8426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4537646,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001668 DATLJT010000000
VERSION     DATLJT010001668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4537646"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VM910_37950"
     CDS             <1..379
                     /locus_tag="VM910_37950"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI68321.1"
                     /translation="PDDFNEAFTVMKDDPPDAILMVADALTTLNRKRVFEFAATRRLP
                     AIYEYDFYVRDGGLMSYGPDLAESFQRAAALVDRIFKGANPADLPFEQPTRYLFVVNL
                     KTAKAADLEMPPTLLALADEVIE"
     gene            435..3035
                     /locus_tag="VM910_37955"
     CDS             435..3035
                     /locus_tag="VM910_37955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HVI68322.1"
                     /translation="MTISLHPDTPQGRTLAAPAIAPAAPAAASSRGKIRSRLFTKYVA
                     LFVAVVGIALLSNGIFEVFFYYREHKAALIRIQHEQAEAAAAKISQFIKEIESQLGWT
                     TQLPWSAGSIEQRRFDALRLLRQVPAITELAQVDSTGKERLRVSRLAMDVVDSGLDLS
                     KDPKFTEAVAHKVYYGPVYFRRESEPYMTLALAGTRKDAGVSIAEVNLKLIWDVVSQI
                     KVGEHGHAYVVGSQGRLIAHPDISLVLRNTDMSKLPQVQAAQAGGDTTSESLEGAKNI
                     QGQEVLTASAPIQPLRWTMFVELPVEEAYASLYAALQRLAVVLFAASLFAVLAGIFLA
                     RRMVGPIQALRAGAERIGGGDFAQRISIKTGDELEGLANQFNDMGARLQESYADLENK
                     VEQRTSELRETLQQQTATSEVLETISSSTGDLEPVFQKMLENATRICGAKFGTMTLYE
                     NGGFRTVALYNAPQAYVDTQLYKLIRPHPEGGLGTVERTHEAVHIDDIRTQAPYIEGN
                     PNVRALSDLAGARTLVIVPMLKEDELIGSITIFRQEVKPFTDKQTELVANFANQAVIA
                     IENARLLNELRERTQELSRSLDDLRTAQDRLIQTEKLASLGQLTAGIAHEIKNPLNFV
                     NNFSALSTELIDELNEVLKSAGIDEKAREEIDELTHMLKGNLEKVVQHGKRADSIVKN
                     MLLHSREGSGERRPADINAIVEESLNLAYHGARAEKSGFNITLKRELDPDAGMIDLYP
                     QEITRVFLNLISNGFYAATKRKETAGDGFEPTLSAATKSLGSRVEIRIRDNGIGIPKD
                     VKEKMFNPFFTTKPAGEGTGLGLSMSHDIVVKQHGGKIDVKTEPGVFTEFIITLPRTL
                     AAQGNTGGTH"
     gene            3032..3421
                     /locus_tag="VM910_37960"
     CDS             3032..3421
                     /locus_tag="VM910_37960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68323.1"
                     /translation="MSVYILVVDDEPDVEALFRQQFRRDLRTGRFQMEFAPSAPAALQ
                     RAVEIGEPSLILILSDINMPGMSGLEMLPEVRARRPDVPVIMITAYGDPETRRKAIER
                     GAEGLLTKPIDFVVLRQEIDTRLEQAA"
     gene            3418..4668
                     /locus_tag="VM910_37965"
     CDS             3418..4668
                     /locus_tag="VM910_37965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI68324.1"
                     /translation="MMATILFVDDEPDLEALVLQKFRKQIREGAVRVIFAHDGVEALE
                     SLERHQVDMVVSDINMPRMDGLSLLQRLQEGEDKKSTIIVSAYGDMNNIRTAMNRGAF
                     DFLTKPIDFGDLETTIDKTIRHIEVLREARRRQAEAERAHASLSRYFSPQLASRLASC
                     NGEDDGMEVQWRDVATIFTDITGFTSLVESAAPEVLGKLLNEYVGGMTDVIFSHEGTV
                     AKIIGDAIQVLFNAPGDQPDFASRAVGCAHALDAWAEDFRKRWKAKGVNFGVTRIGVH
                     AGPALVGNFGGSRFFDYTAYGDTINIAARLESANKFLGTRICVSATVAERAENFQGRP
                     VGDLILRGRSEPLRAYEPLVSEAFKTPAITQYSEAFAKLEAGDATAMPAFAALVGLHA
                     DDPLAGFHLKRLLNGAKGVCMQLE"
     gene            4685..5431
                     /locus_tag="VM910_37970"
     CDS             4685..5431
                     /locus_tag="VM910_37970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68325.1"
                     /translation="MTRDFAAGNYRFIPAVFQYSSGAAADRGYEIERVRFDRLLPLGQ
                     GFAQIAKYIQAAGRPLTSFCACELRSPAAFTEDGFFKFNQHYVKTLAEWGIYDGGSTN
                     PVARSNVCPEIDPPAEPSFYAFSFTRPSRGATSTFVIAGGAELGGGSGSYAERIVRYR
                     DLSPEGLKEKVRFTTRQQESRMEEFGFGWKDATAVQAYTVHDFHPVIVEELIRRGATR
                     PGLTWHFARPPVIDLEFEMDCRRVMREIVI"
     gene            complement(5435..6325)
                     /locus_tag="VM910_37975"
     CDS             complement(5435..6325)
                     /locus_tag="VM910_37975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68326.1"
                     /translation="MSEAALSAIPLAASDAELETPSRRALRRLLGRKGAVAGLVVITM
                     FIVLALFAPLIAPYDPIATSWTLVRKPPSALHWFGTDDLGRDILARVIYGARASLLAG
                     AISVGIALAIGVPLGLLSGYRGGWADALISRITDAMLACPFLILAIALAAFLGPSLGN
                     AMIAIGISATPIFIRLTRGQVMSVKVEDYVEAARAVGNPPRRIALVHILPNIMPALLV
                     QATLSIAAAIIAEAALSFLGLGQQPPSPSWGSMLNAAQRFLSNAPWMAIWPGLAIFLV
                     VLSFNLVGDGLRDALDPRGR"
     gene            complement(6332..7273)
                     /locus_tag="VM910_37980"
     CDS             complement(6332..7273)
                     /locus_tag="VM910_37980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68327.1"
                     /translation="MLNFLARRIVQLIPTLFFVSILIFSLQQLLPGDPALVMAGEERD
                     PAVIAQIRQQYHLDQPIPVQYVYWVKGVLSGDLGESLRIKVPVRELIAQKLPVTLQLA
                     SMAIVIAFLIGIPAGIVSAVKKGTAWDYGANIFALWGISTPNFWLGILLIFLFSIKLG
                     WLPASGYVPPWENLRASLASTIMPAFVLGNAIAAILMRHTRSAMLHVLESDYVRTARA
                     KGLSERSVILKHAMRNALTPVITLGALELGTLLSGAVLTEQIFSIPGFGKLVVDAVFN
                     RDYAVVQGVVLVTATIYITLNLVADIAYILVNPRLRG"
     gene            complement(7278..>8426)
                     /locus_tag="VM910_37985"
     CDS             complement(7278..>8426)
                     /locus_tag="VM910_37985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68328.1"
                     /translation="KPELASLDHVEVVDPLTIKLVLKAPFSPLIAQLTDRAGMMVSPK
                     AAREAGDKFGLHPVCAGPYKFVERVQQDRMVFEKFADYWNKDNVFIDRIVYLPIVDAT
                     VRLANLKSGSLDLIERVLATDIKDVRADNRLKLSTAIELGYQGITLNIGNDKAKGPLS
                     QSAKVRQALDLSIDREAINQVVFNGEFMPGNQWVNPEHPYYQKAFPIRPRDVEKAKVL
                     LKEAGATPPVSVDFMVPKGAETEAVAQVVQSMAAEAGFDMKIRVTEFATSLKQAEAGE
                     YQAYLLAWSGRIDPDGNSYVFLHGKAPQNYSVWANADADKALDDARLVTDMAQRKAIY
                     EKLAKHELNDEAILYIYHRRILIAHTTKLEGYKQMPDGLVRVVGLKLK"
BASE COUNT         1651 a         2596 c         2681 g         1498 t
ORIGIN      
        1 gccggacgat ttcaacgagg cattcacggt catgaaggac gatccgcccg acgcgatcct
       61 gatggtcgcg gacgcgctga cgaccctcaa ccgcaagcgt gtgtttgagt tcgcagcaac
      121 cagaagactg ccggccatct acgaatatga cttttacgtt cgcgatggcg ggctgatgtc
      181 ttacgggccc gatctggcgg aatcgttcca gcgagcggcg gcattggtcg accgcatctt
      241 caaaggggcc aatcctgccg acctgccgtt cgagcagccg acccgctacc tcttcgtcgt
      301 taacctcaag acggccaagg ccgccgacct ggaaatgccc ccgaccctcc tggcccttgc
      361 ggacgaggtc atcgagtaat cttccgcacc gcggcaagag acaagcccgg gccttcttgc
      421 taaattgagg cccgatgacg atttccctcc accccgacac gccccagggc aggacgctgg
      481 cggcgccggc gatcgccccc gcggcgcccg cagcggcgtc gtcgcgcggc aagatccgca
      541 gccggctgtt cacgaaatac gtcgcgctgt tcgtcgcggt ggtcggtatc gccctgctgt
      601 ccaacggcat tttcgaggtt ttcttttatt accgcgagca caaggcggcc ctgatccgga
      661 ttcagcacga gcaggccgaa gctgcggcgg ccaagatcag tcagttcatc aaggaaatcg
      721 agagccagct cggctggacg actcagttgc catggtctgc gggctcgatc gagcagcggc
      781 gattcgacgc gctgcggctg ctccgtcagg ttccggcgat cacggagctc gctcaggtcg
      841 attcgaccgg aaaggagcgg ttgcgggttt cccgccttgc gatggacgtt gtcgacagcg
      901 gcctcgacct ctccaaggac cccaagttca ccgaggccgt cgcgcacaag gtctattacg
      961 ggccggtcta tttccgccgc gaatccgagc cctatatgac gctcgctttg gccggcacgc
     1021 gcaaggacgc cggagtcagc atcgccgaag tcaatctgaa actgatctgg gacgtcgtct
     1081 cccagatcaa ggtcggcgag cacggccacg cctatgtggt gggctcgcaa ggccgcctga
     1141 tcgcgcatcc cgacatcagc ctggtgctgc gcaacaccga catgtccaag cttccgcagg
     1201 tgcaggcggc gcaggccggc ggcgacacga cgtccgagtc gctcgaaggc gcgaaaaata
     1261 tccagggtca ggaggtgctc accgcgtctg cgccgatcca acccttgcgc tggaccatgt
     1321 tcgtggagtt gccggtggaa gaggcctatg cctcgctgta cgcggcgctg cagcgcctgg
     1381 ccgttgtgct gttcgcagcc tcgctcttcg cggtgctcgc agggatcttc ctggcgcggc
     1441 gcatggtcgg accgatccag gccttgcgtg ccggcgccga gcgcatcggc ggcggcgatt
     1501 ttgcgcagcg catctcgatc aagaccggcg acgagctgga ggggcttgca aaccagttca
     1561 atgacatggg cgcgcggttg caggaatcct atgccgacct ggagaacaag gtcgagcagc
     1621 gcacctcgga attgcgggaa acgttgcagc agcagacggc gacctcggag gttctcgaaa
     1681 ccatcagctc gtccaccggc gatctcgagc ccgtcttcca gaagatgctg gagaatgcga
     1741 cccggatctg cggcgcaaaa tttggaacga tgaccctgta cgaaaacggc ggctttcgga
     1801 ccgtcgcgct ctacaacgcg ccgcaagcct atgtggacac ccagctctac aagctcattc
     1861 ggccgcatcc cgaaggcggg ctcggcacgg tcgaaaggac ccatgaggct gtccacatcg
     1921 acgacatccg gacgcaggcg ccctatatcg agggcaatcc caatgttcgc gcactttcgg
     1981 atcttgccgg cgcccgcacg ctggtgatcg tgcccatgct caaggaagac gaactgatcg
     2041 gctcgatcac gattttccgt caggaagtga aaccgttcac agacaagcag accgaactgg
     2101 tggctaactt cgccaaccag gccgtgatcg cgatcgagaa tgcgcgcctg ctcaacgaat
     2161 tgcgcgaacg tacgcaggaa ttgtcgcggt cgctcgacga tctgcgcacc gcacaggacc
     2221 gactgatcca gaccgaaaag ctcgcttcgc tcggtcagct cactgcgggc atcgcccacg
     2281 aaatcaagaa cccgctgaac ttcgtaaaca acttctcggc gctgtcgacg gaattgatcg
     2341 atgaactgaa cgaggtgctg aagtccgccg ggatcgacga aaaggcacgc gaggaaatcg
     2401 acgagctcac ccatatgctc aagggcaatc tcgaaaaagt cgtgcagcac ggcaagcgtg
     2461 ccgactccat cgtcaagaac atgctcctgc attcgcgcga ggggagcggc gagcgtcgcc
     2521 cggccgacat caatgcgatc gtcgaggaga gcctcaatct tgcctatcac ggcgcgcggg
     2581 cggaaaagag cggcttcaac atcacgctca aacgcgagct cgatcccgac gccggcatga
     2641 tcgatctcta tccacaggaa atcacgcggg tcttcctcaa cctgatctcc aacggctttt
     2701 acgccgcgac caaacgcaag gagaccgcgg gtgacggctt tgaaccgacc ctgagcgcgg
     2761 cgaccaagag cctcggcagc agggtcgaaa tcagaattcg cgacaacgga atcggcatcc
     2821 ccaaggacgt caaggaaaaa atgttcaatc cgttcttcac caccaagccg gccggcgagg
     2881 gaaccgggct tgggctgtcg atgagccacg acatcgtcgt gaaacaacat ggcggcaaga
     2941 tcgacgtaaa aaccgaaccg ggcgtcttta ctgaattcat tattacgctg ccgcgtaccc
     3001 tggcagcaca gggaaatact ggaggcacgc attgagcgtt tatatcctcg tcgtcgacga
     3061 tgagccggac gtggaggcgc tatttcgcca acagtttcgg cgcgacctgc ggacgggcag
     3121 gtttcagatg gaattcgcac cttcggcgcc ggccgcgctt cagcgggcgg tcgagatcgg
     3181 agagccctcg ctgatcctga tcctgtctga tatcaatatg cccggcatga gcgggctcga
     3241 gatgctgccc gaggtgcggg cgcggcgtcc ggacgtgccc gtgatcatga tcaccgccta
     3301 tggcgatccg gagacacgca ggaaagcgat cgagcgcggc gctgagggac tgttgaccaa
     3361 gccgatcgac tttgtcgtcc tgcgtcagga gatcgatacc aggctcgagc aggctgcatg
     3421 atggcgacca ttctgtttgt cgatgatgag cccgacctcg aggcgctggt cctgcagaaa
     3481 ttccgcaagc agatccgcga gggcgcggta agggtgatct tcgcccatga cggcgtcgag
     3541 gcgctggaat cgctcgagcg gcatcaggtc gacatggtgg tgtcagatat caacatgccg
     3601 cggatggacg gcctgtcgct gttgcagaga ttgcaggagg gcgaggacaa gaagtcgacc
     3661 atcatcgttt ccgcctatgg cgacatgaac aacatccgca ccgcgatgaa ccgcggcgca
     3721 ttcgacttcc tgaccaagcc gatcgatttc ggcgatctcg aaaccacgat cgacaagacc
     3781 atccgccata tcgaggtgct gcgcgaggcg cgccgccggc aggccgaggc ggagcgggcg
     3841 cacgcgtcgc tgtcgcgcta tttctcgccg cagctcgcaa gccgccttgc ttcttgcaac
     3901 ggcgaagacg acggcatgga ggtgcaatgg cgcgacgtcg ccacgatctt caccgacatc
     3961 accgggttca cctcgctggt cgagagcgcg gcgcctgagg ttcttggcaa gctgctcaac
     4021 gagtatgtcg gcggcatgac cgacgttatc ttctcgcatg agggcacggt ggcaaagatc
     4081 atcggtgatg ccatccaggt tctgttcaac gcgcccggcg accagccgga tttcgcctcg
     4141 cgcgcggtcg gctgtgccca tgcgctcgat gcctgggcgg aggattttcg caagcgctgg
     4201 aaggccaagg gtgtgaattt cggcgtgacc cgaatcggcg ttcacgcggg gccggcactc
     4261 gtcggaaatt tcggcggcag tcggttcttc gattacacgg cctatggcga caccatcaac
     4321 atcgccgccc ggctggaatc ggccaacaag ttcctgggga cgaggatctg cgtcagcgcg
     4381 acggttgccg agcgggcgga gaactttcag ggcaggccgg tcggcgatct catcctgcgc
     4441 gggcggagcg agccgcttcg cgcctatgag ccgctggtga gcgaggcctt caagacgccg
     4501 gcgatcacgc aatattcgga agccttcgca aagctcgagg cgggagacgc gacggcgatg
     4561 ccggccttcg ccgcgctggt tggcctgcac gccgacgatc cgctggcggg cttccatctc
     4621 aagcgactgc tgaacggcgc caagggcgtc tgcatgcaac tcgagtaacg caggacaagc
     4681 cgacatgacg cgcgattttg ccgccggcaa ctaccggttc attccggccg tgttccaata
     4741 ttcgagcggc gccgcggcgg accgcggcta tgagatcgag cgcgtgcggt tcgacagact
     4801 gctgccgctg gggcaaggat ttgcgcagat cgcaaaatat atccaggcgg cgggacggcc
     4861 gctgacgtcg ttttgcgcct gcgagctgcg gtcgccggcg gcgttcacgg aggacgggtt
     4921 tttcaagttc aaccagcact acgtcaagac gctcgccgag tggggcattt acgacggcgg
     4981 cagcaccaat ccggtcgcgc gcagcaatgt ctgccccgag attgatcctc ccgccgagcc
     5041 atcgttctac gccttttcct ttacgcgtcc gagccggggc gcgacatcga cctttgtcat
     5101 cgccggcggc gccgagcttg gcggtggcag cggaagttac gcggagcgca ttgtgcgtta
     5161 ccgcgatctg agccccgagg gattgaaaga aaaggtccgc ttcacgacgc ggcagcagga
     5221 aagccgtatg gaggagttcg gcttcggatg gaaagacgcc acggccgtcc aggcctatac
     5281 ggtgcacgat ttccatccgg tgattgtcga agaactgatc cgccgcggcg cgacccgacc
     5341 cggcctgacc tggcatttcg cccgcccgcc cgtgatcgat ctcgaatttg agatggattg
     5401 ccgccgcgtg atgcgggaga tcgtgatcta ggcgctagcg tccgcgcgga tcgagcgcgt
     5461 cgcgcaatcc gtcgcccacg agattgaacg acagcaccac cagaaagatg gcaagtcccg
     5521 gccagatcgc catccagggc gcgttcgaga ggaatcgctg cgcggcgttc agcatgctgc
     5581 cccaggaggg cgagggcggc tgttgtccca gtccgagaaa cgagagcgcg gcttccgcga
     5641 tgatcgcggc cgcaatcgac agcgtcgcct gcaccaggag cgccggcatg atgttcggca
     5701 ggatatggac cagcgcgatc cgccgcggcg gattgcccac ggcgcgtgcg gcctcgacat
     5761 aatcctccac cttcacgctc atgacctggc cacgcgtgag gcggatgaag atcggagtgg
     5821 cggaaatgcc gatcgcgatc atcgcattgc cgaggcttgg gccgaggaaa gcggcaagcg
     5881 cgatggcgag aatgaggaac ggacaggcca gcatcgcgtc ggtaatgcgg ctgatcaacg
     5941 cgtcggccca tccgccgcga tagcccgaca aaagcccgag cggcacgccg atggcgagcg
     6001 caatgccgac cgagattgcg ccggccagga gcgatgcgcg cgcgccgtag atgacgcggg
     6061 cgaggatatc gcggccgaga tcgtcggtgc cgaaccagtg caatgcggag ggtggcttgc
     6121 gcaccagcgt ccagctcgtt gcgatgggat cgtaaggcgc gatcagcggc gcaaacaacg
     6181 ccaggacgat gaacatcgtg atcacgacca ggcccgcgac cgcccccttg cgcccgagca
     6241 ggcgccgcag cgcgcgccgc gaaggcgtct ccagctcggc gtcggaggcg gcaagcggga
     6301 tggctgacag cgcggcctcg ctcatggtgc ctcagcccct caacctggga ttgacgagga
     6361 tataggcgat gtcggcaacg aggttgagcg tgatgtagat cgtcgccgtc accagcacca
     6421 cgccttgcac cacggcataa tcgcggttga acacggcgtc gacgaccagt ttgccgaaac
     6481 ccggtatcga gaaaatctgc tcggtcagca cggcaccgga gagcagcgtg ccgagctcga
     6541 gcgcacccag cgtgatgacc ggcgtcagcg cattgcgcat ggcatgcttg agaatgaccg
     6601 agcgttcgga aagccccttt gcgcgggcgg tgcggacata atcgctctcc agcacgtgca
     6661 gcatggcgct gcgggtgtgg cgcatcagga tcgcggcgat cgcgttgccg agcacgaaag
     6721 cgggcatgat ggtggaagca aggcttgcac gaagattttc ccacggcggc acgtagccgg
     6781 acgccggcag ccagccgagc ttgatggaga acaggaagat caggaggatt cccagccaga
     6841 aattcggcgt cgagataccc cacagcgcga agatattggc gccgtagtcc caggcggtgc
     6901 ccttcttcac ggccgaaacg atgcccgcgg ggatgccgat caggaacgcg atcacgatcg
     6961 ccatcgaggc gagttggagg gtcacgggca gtttctgcgc aatcagctcg cgcacgggta
     7021 ccttgatgcg cagggattcg ccgaggtcgc ctgacaggac gcccttgacc caatagacat
     7081 attgcaccgg gatcggctga tcgaggtggt actgctggcg gatctgcgcg atcacggccg
     7141 gatcgcgctc ttcgcccgcc atcaccagcg ccggatcgcc gggcagcagt tgttgcagcg
     7201 aaaagatcag gatcgacacg aagaacagtg tcggtatcag ttgcacgatg cggcgggcga
     7261 ggaagttgag catatcctca cttcagcttg agcccgacca cgcgcaccag gccgtcaggc
     7321 atctgcttgt acccttcgag cttcgtcgtg tgcgcgatca ggatcctgcg atggtagatg
     7381 tagagaatgg cttcatcatt cagctcgtgc tttgccagct tctcgtagat ggccttgcgc
     7441 tgcgccatgt cggtcacaag tcgcgcatcg tcgagcgcct tgtcggcgtc tgcattggcc
     7501 catacgctgt aattctgcgg cgccttgccg tgcaggaaca cgtaggaatt gccgtccgga
     7561 tcgatgcggc cgctccacgc cagcagatag gcctggtact cgccggcttc cgcctgcttc
     7621 agcgaggtcg caaattccgt gaccctgatc ttcatatcga agccggcttc cgccgccatc
     7681 gattgtacga cctgcgcgac cgcctcggtc tcggcgccct ttggcaccat gaagtcgacg
     7741 gatacgggcg gcgtggcgcc ggcctctttg agcagtactt ttgccttctc cacgtcgcgt
     7801 gggcggatcg ggaaggcttt ctggtaatag ggatgctccg gattgaccca ttggttgccc
     7861 ggcatgaatt cgccgttgaa cacgacctgg ttgatcgctt cgcggtcgat cgaaagatcg
     7921 agcgcctgcc gcactttcgc cgactggctg agcggcccct tggccttgtc gttgccgatg
     7981 ttgagcgtga tgccctgata gccgagctcg atcgctgtgg agagcttcag cctgttgtcg
     8041 gcgcgcacgt ccttgatgtc ggtggcaagc acgcgctcga tcagatcgag gctgccggat
     8101 ttcaggttgg ccagccggac tgttgcatcg acgatcggca aatagacgat gcggtcgata
     8161 aacacgttgt ccttgttcca gtagtcagcg aacttctcga acaccatgcg gtcctgttgc
     8221 acgcgctcga cgaatttgta gggtccggca cagaccggat gcagcccgaa cttgtcgccg
     8281 gcttccctcg cggccttcgg cgacaccatc atgccggcgc ggtcggtaag ctgcgcgatc
     8341 agcggcgaaa acggcgcctt gagcaccagc ttgatggtaa ggggatcgac gacttcgaca
     8401 tggtcgagcg atgccagttc gggctt
//
LOCUS       DATLJT010001669         6067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3390285,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001669 DATLJT010000000
VERSION     DATLJT010001669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390285"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1231
                     /locus_tag="VM910_37990"
     CDS             371..1231
                     /locus_tag="VM910_37990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI68329.1"
                     /translation="MAEISPLRRRMIEDMTVRNLSPATQRSYLAAVLKLSRYFGRSPD
                     CLELEDIRAFQVHLVATGISWPALNQIVCALRFFYGVTLGHDAIPERIAYAREPRKLP
                     VVLSADEVVRFLEAIPSLKSRTALTTVYAAGLRVSEVVMLKIADIDSQRMVIRVEQGK
                     GGKDRYVMLSPQLLRILRSYWRFARPQRWMFPGRDEDRPLDPTVLHAACRSARSAADL
                     SKQVTVHTLRHSFATHLLENGADVRIIQVLLGHASLASTARYTQVATKTISNTPSPLD
                     RLRLEVVPPG"
     gene            1241..1432
                     /locus_tag="VM910_37995"
                     /pseudo
     CDS             1241..1432
                     /locus_tag="VM910_37995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(1811..2974)
                     /locus_tag="VM910_38000"
     CDS             complement(1811..2974)
                     /locus_tag="VM910_38000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVI68330.1"
                     /translation="MSQVSDLSRSLVPFDQNITLTVVVELSEASWLVAGLIPGVDRQP
                     VKKQEPEPVALLRLLERWRVEAVRAGRTIGRIALAFEAGRDGFWLARWLQARDIEVHV
                     VHSTSVAVSREHRRAKTDRLDTAMLMRVFLGWLRGERGHCRMVAIPTLEQEDAKRPSR
                     ERESLVGERTRIVNRMKAALARLGIRGFKPTLRKAPQRLDALRTPEEVSIPPNTLDEI
                     RRDMVRLAVVREQIDVIERARLARLEQAPAAGPHAMVRLLARVIGVGLETADMLVQEV
                     LSRNLRDRRAVARYVGLTGSPDESGRRRREKGLAKAGNARVRRGLIQLAWRFLRFQRD
                     STLAQWYRTRTGGPKGARKTTMIVALARKLLIALWRMVTIGEIPAGVALRQVI"
     gene            3046..4032
                     /locus_tag="VM910_38005"
                     /pseudo
     CDS             3046..4032
                     /locus_tag="VM910_38005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            4410..5759
                     /locus_tag="VM910_38010"
     CDS             4410..5759
                     /locus_tag="VM910_38010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HVI68331.1"
                     /translation="MIHGWSALGGLTMAEWEDELERWLMPFLDRLGHKTRQRMCPLYV
                     AGLIGPGDRKSVQPMAERLATGNYDQLHHFIADGVWDASPLESELLNQADRLVGGKDA
                     VLVIDDTAIPKKGERSVGVAAQYASALGKTANCQTLVSLTLARSEVPVMVALRLFLPD
                     SWTSNVSRLKRARVPAEHRTPRSKPEIALAEIDRAMAANVRFGCVLADAGYGLSAPFR
                     QGLTERGLAWAVGIPRHLKVYPVNVQLIWPITKVRGKPRKHHVPDILSIAAEQMLASA
                     KWKTVSWRSGTKGRLKARFAALRVRTADGPPQRIWDKGQQHLPGDEAWLIGEQRASGE
                     KKYYLANLPAATDLRTLAATIKARWVCEQAHQQLKEELGLDHFEGRSWQGLHRHALMT
                     MIAYAFLQHRRLAFAGRKKKNQRTSASANHARRTSRHCRSHPSAAVSTVSILPKTNH"
     gene            complement(5796..5948)
                     /locus_tag="VM910_38015"
                     /pseudo
     CDS             complement(5796..5948)
                     /locus_tag="VM910_38015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1125 a         1985 c         1796 g         1161 t
ORIGIN      
        1 gcggcggcgg tgaaactggt gcgcgtgcgc gggttggttc tgttgttggg ccgccacccg
       61 catctgaatg ttcaagcccc gaacgaattt gagttggtcg tgaaccgccg ctcggacgag
      121 ctgatcaaat aggacccgat acgttgcgat gcatgagtcc ggaaggggcc cttcggcgac
      181 ttgtccggac gcctcgtcga tgtctggata gtggggggta tagcggacta gaggagcatg
      241 ttgtttttta ccggaatcgg gattgaacga agccgctggt gcggcaatta aggaaggatc
      301 gcaatagctt cctcctgtct gagaggatcg cgggttttcg aagcccaacc cttcagacag
      361 gagttttcca atggctgaga tcagccctct tcgccgccgc atgatcgagg acatgacggt
      421 ccgcaatctg tcgccggcga cgcaacgatc ctaccttgcc gcggtattga agttgagccg
      481 atacttcggc cgatcccccg attgtctcga actggaggat atccgcgcct tccaggtcca
      541 cttggtagcg accgggatat cctggccggc gctgaaccag atcgtctgtg cgctgcgctt
      601 tttctacggt gtgacgctcg gccatgacgc cattccggag cgcatcgcct atgcgcgcga
      661 gccgcgcaag ctgccggtcg tgctaagcgc tgacgaggtc gtgcgcttcc tggaggcaat
      721 cccaagcctc aagagccgca cggcgctgac gacggtctat gccgccggac tgcgggtgtc
      781 ggaggtggtg atgctgaaga tcgccgacat cgacagccag cggatggtga tccgggtcga
      841 gcagggtaag ggaggcaagg atcgttacgt catgctctcg ccgcagctgt tgaggattct
      901 gcgcagctat tggcggttcg cccgaccgca gcgctggatg ttccccggtc gtgacgagga
      961 ccgaccgctt gaccccaccg tattgcacgc ggcctgccgt tcggcccgct cggcggccga
     1021 cctgagcaaa caggtgacgg tccacacgct gcgacacagc ttcgcgacgc atctgctgga
     1081 gaacggggcc gacgtgcgca tcatccaagt gcttctcggc catgccagcc tggccagcac
     1141 agcgcgctac acccaggtcg ccaccaagac catcagcaat acgccaagtc cgctcgaccg
     1201 gcttcgtctg gaggtcgtgc cgcccggctg agcgggtacc atggctgcgg tcccggaggt
     1261 ggcggatgtc ttccgccgcc acggtgaagc gtttcggcag gcccgtgccg ggcatctcgg
     1321 ccgcgtcgag cggcgcgtca tgggcgccat cacagcgtgc cggacggcgg cgctcggcgg
     1381 ccacgtcgag cagtgcgacg actgcggcgc gacgcgcatc gcctacaact ccttgtatgg
     1441 ggatttattt gtcaagctct cagcctcctc tttcttgaat ccctgacggc ctgcgcctgg
     1501 ccaaaaggcc ggggtcgatc gaccgcgagg gtgaggcggg cgttagcccg atcagcccac
     1561 tgtgcgagca atccgagggg agcctgccat tgggcgcaaa gcattgcgcg cgaccttgta
     1621 ttcggttgga ttcgtatcgg acccggacca tgaagcagtc aatggcttca gcagcaaggc
     1681 tccgaggcgg cgggcccatt ctgtcgacgg cattggagcc atggagttgt tcgggtcacc
     1741 gccacctcga atcgctacac gtggcctcct cgcgacagtc gatccgccgc gcgacgacca
     1801 tgccgcgtgt tcagatcacc tgccgcagcg cgacgcccgc cgggatctcg ccgatggtga
     1861 ccatccgcca tagcgcgatg agcagtttgc gggcgagtgc cacgatcatt gttgtcttgc
     1921 gcgcgccctt cgggccaccg gtccgcgtcc ggtaccattg cgcaagcgtg ctgtccctct
     1981 ggaatctcaa gaaccgccag gcgagttgga tcaggccgcg gcgcacgcgg gcattgcctg
     2041 ccttggccaa tcccttctcc cgtcgccgcc tgccactctc gtcaggcgaa cccgtgaggc
     2101 caacataacg ggccaccgct cgccggtctc gaaggtttcg ggacaatacc tcctgaacca
     2161 acatgtccgc cgtttccagg ccgacgccga tgacgcgggc caacaggcgc accatggcgt
     2221 gtggccccgc cgccggcgct tgttccaggc gcgcgaggcg ggcccgttcg atcacatcga
     2281 tctgttctcg caccaccgcg agccgaacca tgtcgcgtcg gatctcgtcg agcgtgttcg
     2341 gtggaatcga cacctcctcc ggcgtgcgca aagcgtcgag ccgctgcggc gccttgcgca
     2401 acgtcggttt gaacccgcgg atgccgagcc gggccaaggc tgccttcatc cggttgacga
     2461 tccgcgtgcg ctcgccgacc aggctttccc gttcacggct cggacgcttg gcgtcctctt
     2521 gctcgagcgt tgggatcgcc accatccggc agtgcccgcg ctcgccgcgc agccatccca
     2581 ggaacacacg catgagcatc gccgtatcca gccgatcggt cttggctcgg cgatgctcgc
     2641 gtgacacggc gacgctggtt gagtgaacaa cgtgcacctc gatgtcgcga gcctgtagcc
     2701 accgcgccag ccagaacccg tcgcggcccg cctcaaaggc gagggcaatc cgtccaattg
     2761 tcctgccagc cctcaccgcc tccacccgcc aacgctccaa cagccgcagc agcgcgaccg
     2821 gctccggttc ctgcttcttc acgggttggc gatcgacacc gggaatcaac ccagcgacga
     2881 gccagctggc ctcgctcagc tcaacgacca ccgtcagagt gatattttga tcaaagggaa
     2941 caagggaacg gctcagatca ctgacttggc tcatggggca cctccatccg tttcatcgaa
     3001 cgacggtgga ccatttcacg acctcgccgc tctccccata gcttctgtcg taatcggcat
     3061 tgcccgaagt gccagggctc ggcacgcgcg caatggctcg ccaaacggca agccgaactc
     3121 cttcccgtgc cgtatttcca cgtcgtcttc accttgccgg cgcccgccgg agagattgcc
     3181 ttccaaaaca aagccgtcgt ctatgccatc ctgttccgct gcgcggccga gacactcgcc
     3241 accatcgcgg ccgaccccaa gcatctcggc gcccaactcg gcgtgaccgc cgtcctccat
     3301 acctggggcc agaccctgca acaccatccc cacctccatt gtgtcgtgcc tggtggtgga
     3361 ccttcgctcg acggcacacg ctgggtcgct tgccggcccg gcttcttcct gccagtacgc
     3421 gtcctctccc ggctattccg ccgcctgttt ctgcaagagc tacaagccgc cttcgcggct
     3481 ggccagtttg gcttctttgg cgatctcgcc catctcgccg atccccgcgc gttcacccag
     3541 cggctcgatc agctcagacg gaccgactgg gtcgtctatg ccaagccacc attcggcggg
     3601 cccgaacagg tgctggctta tctcggccgc tacacgcatc gcgtcgccat cgccaacagt
     3661 cggttgattt cgcttgccga tggcaaggtg agcttttcct ggaaggacta tcggcagaac
     3721 cgcaaaacca aagtgatgac gcttgatgcc gacgagttca ttcgtcgctt tctgctgcac
     3781 acactgccgg acggcttcca ccgcatccgc cactacggct ttctcgccaa cggcggacgc
     3841 agcgacaaaa tcgccctctg ccgccaactc ctcgcaatcc gcaacgctgc gactgatcaa
     3901 caagccggcg aggatcccct cgtcaaatgc gagatccccg tctgcccaca ttgcggcggc
     3961 accatgcggc gcgtcgatgt cgtcccacga gcctgcacac gcgaccagcc gtttcgttgt
     4021 gatacgtcat gaccagcgcc tgcaccatcc accccttccg tgatcacctt ctcggtggca
     4081 acgtccgatg tcgccacgac cgcgttcgtc accaaacatc ccgccaatcg ttcggccgcg
     4141 catcccagcg ccgcaaatcc ctcgctggat cgcgctcctt gcctgatcaa tggcaccgct
     4201 cacatccggt gaatgtctcg ccgacgcgcg gcgaccacag gcgcgatttc cccgcaaccg
     4261 caccggctgc attatcccca tagcgccgtc cacaactccg cagcttcgtt caatccggct
     4321 tctatgaggt cgccacccgc ggcacagcgc gcggcacggt ctggcacgcg acctcacaga
     4381 accctcaaga ttcccgaatc gatttttcga tgattcatgg gtggtcggct cttggagggc
     4441 tgaccatggc ggaatgggaa gacgaactcg aacgctggct gatgccgttc ctggaccggt
     4501 tgggtcataa gacccggcag cggatgtgtc cattgtacgt tgcgggattg atcggtcctg
     4561 gcgatcgcaa gagcgttcag ccgatggcag aacgccttgc aaccggcaac tacgatcagc
     4621 tgcaccattt catcgccgat ggtgtctggg acgcatcacc gctggaatct gaactgctca
     4681 accaggcgga ccggcttgtc ggcggcaagg atgcggtgct ggttattgat gacaccgcaa
     4741 tcccgaagaa gggcgaacgc tcggttggtg ttgcagctca atatgcgtcg gctctcggca
     4801 aaacggcaaa ttgccaaacg ctggtgtctt tgacgcttgc gcgaagtgaa gtgccggtga
     4861 tggtcgcgtt gcgcctcttc ctgcccgaca gttggacaag caacgtgtct cgtctaaagc
     4921 gcgctcgcgt gccggccgaa caccgaacgc cacggtccaa gccggaaatt gctttggccg
     4981 agattgaccg cgcgatggca gccaacgtgc gtttcgggtg tgtgctggcg gatgcaggat
     5041 acgggctcag cgcaccgttt cgacaagggc taacagaacg cgggctggcc tgggccgtcg
     5101 gcatccctcg gcatctcaag gtatatccgg ttaatgtaca gctcatttgg ccgattacca
     5161 aagtccgcgg gaaaccacga aagcaccacg taccagatat cttatccatt gcggcagaac
     5221 aaatgctggc cagcgccaaa tggaaaaccg tgagttggcg cagcgggacg aagggtcggc
     5281 tgaaagcccg atttgctgct ctccgtgtcc gcaccgccga cggacctccg cagcggatat
     5341 gggataaggg tcagcagcat ctcccaggcg acgaagcttg gctcattggc gagcaacgcg
     5401 cttcagggga gaagaaatac tatctcgcca acctgcccgc ggcgacagat ctgcgcacac
     5461 tggccgccac gatcaaggca cgatgggttt gtgagcaggc gcatcagcag ttgaaggagg
     5521 agcttggact tgatcacttc gaagggcgat catggcaagg tcttcatcgt cacgccctga
     5581 tgacgatgat tgcctacgcc tttttgcaac accgtcgcct cgcattcgcg gggcggaaaa
     5641 aaaagaatca acggacctcc gcctcagcca accatgcccg ccgtacgtca cgccattgtc
     5701 gatctcatcc ttcggccgcc gtttcgacgg tgtccatact gccgaaaaca aatcattgaa
     5761 aaacactggc gcaaatagat tctgccaaag tagtgttagg acgtggactc attagcgccg
     5821 agccatagcc tgattgaagc gagttggacg aaggcgaggt agttggcggc aagtttgtcg
     5881 taacgcgtcg cgacccgacg agattgcttg atcctgttga agaaccgctc aactcgattg
     5941 cgggcgcgaa ggctgcaggt agatcgcgca atggcgtctc ggatcgtaag atcccgaaga
     6001 tgccgttgag tacacggcgg tcattcaccc gcggtacgac gcgcggcttg ttcggcaaca
     6061 ttggctt
//
LOCUS       DATLJT010001670         8432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1324173,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001670 DATLJT010000000
VERSION     DATLJT010001670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324173"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VM910_38020"
     CDS             complement(<1..530)
                     /locus_tag="VM910_38020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68332.1"
                     /translation="MKKGALALLIHGGTDNWSPERWKARFDEVCPDRAVHLLPRAEFD
                     PAEVRYAAVWKPVPGELAAFPNLKVIFNLGAGVDALMSDKTLPDVPLVRVAVADLTDR
                     MTEYVVLHVLMHHRQEPYLRQSQREKRWAPKFQWPASAISVGIMGLGRLGTNAADSLR
                     RIGFRVAGWSRSRKEI"
     gene            669..1037
                     /locus_tag="VM910_38025"
     CDS             669..1037
                     /locus_tag="VM910_38025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVI68333.1"
                     /translation="MLYAILCYHDEDFVGSWTKEQDAAVMTKLAAVQEKLHKQGRLGP
                     VARLLPTTAATTLRKENPPLVLDGPYAETKEQLLGFYLVDCKNLDEALDVARDLGAAN
                     PGGAYEIRPVGMFAPGGALK"
     gene            1034..2311
                     /locus_tag="VM910_38030"
     CDS             1034..2311
                     /locus_tag="VM910_38030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVI68334.1"
                     /translation="MTDAAWIDAALTSARPQAVGALLRYFRNLDTAEEAFQNACLRAL
                     KSWPQNGPPRDPASWLIMVGRNAAIDEVRRGRRQEPLPEDDRSISDLDDTEEALAERL
                     DGSHYRDDILRLLFICCHPDLPPTQQIALALRIVSGLTVRQIARAFLVTEAAMEQRIT
                     RAKARVADAGVPFETPGAVERSQRLAAVAAMIYLIFNEGYSASGDTADTRKPLCEEAI
                     RLARLLLRLFQSEPEIMGLTALLLLQHARADARFDAGGTLILLEDQDRSKWNQKLIAE
                     GLALIDKAMRHRQSGPYQIQAAIAALHSRAERPEDTDWKQIDLLYGALELVQPSPVVT
                     LNRAVAVSKVQGAQAALDMIEPLAPRLSNYFHFFGVRGAFLIQLGRNEEARVAFDRAI
                     ALANTSAEAAHIRMHLDRLMRDSKPREKKAKTD"
     gene            2735..3148
                     /locus_tag="VM910_38035"
     CDS             2735..3148
                     /locus_tag="VM910_38035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVI68335.1"
                     /translation="MPTMIEAKPVSKPALFMGRLVSGLVILFLLFDGAMKLVPLQVVT
                     ETMDRVGYGSSETLARSLGLITIVCTLLYSFPPTSFVGAILLTGYLGGAVASHVRIGS
                     PLFTHILFGIYLGVMVWGGLWLREGTVRAMLPFDR"
     gene            3188..3724
                     /locus_tag="VM910_38040"
     CDS             3188..3724
                     /locus_tag="VM910_38040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVI68336.1"
                     /translation="MFEVIIIIAVLLALAIAAILMLAAARPDTFSVRRTATMNASAEK
                     IFALISDFRQWGRWSPWETRDPGMKRTYDGAERGKGAVYAWDGNKNVGAGRMEILEAA
                     TPSKALIKLDFIKPFEGHNTAEFTMLPQGSGTTVTWVMQGPAPFMAKLMHVFVNMDRM
                     LGKDFEAGLANLKKQVES"
     gene            3779..4192
                     /locus_tag="VM910_38045"
     CDS             3779..4192
                     /locus_tag="VM910_38045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68337.1"
                     /translation="MQVNPYVYYDGNCEAAFKFYEKELGARIEMMLRYEEAPADMPSS
                     PEHKKKIMHARISIDGATIMGSDAPPKHFHKPQGFSLSLTVDDPAEAERKFNALCKGG
                     AVNMAWSKTFFAKGFGMGVDQFGIPWMVISPLPNS"
     gene            complement(4196..4639)
                     /locus_tag="VM910_38050"
     CDS             complement(4196..4639)
                     /locus_tag="VM910_38050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68338.1"
                     /translation="MGITRIIFVVGLAIALWFFATLLARADVRILSSPGGQVGPFLDL
                     FEEVNRSGERVVIDGPCLSACTLVLSMVPSDRICVTRRAVFGFHAARSMDRRGRLYAE
                     PEASKVVLEAYPDPIRSWIVQRGGLTSRLLLLRGRELAAMYRTCR"
     gene            complement(4644..5432)
                     /locus_tag="VM910_38055"
     CDS             complement(4644..5432)
                     /locus_tag="VM910_38055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68339.1"
                     /translation="MAASPQNVALVTGAARGIGLATAKRFLAEGWRVALLDIEDELLR
                     GAVDGLADPDNTLPLHCDVSDAAAVEAAIDALRKRFGRLDALINNAGVAVFAPLMETA
                     DADWNRILAVNLTGPFLFAKAAVPLLREGGGGAIVNITSISAVRASTLRSAYGTSKAA
                     LAHLTKQLAVELASSGIRVNGVAPGPVDTAMAKEVHTPEIRADYHDAIPLNRYGLEEE
                     LAEAIYFLSCDRSSYITGQILAVDGGFDAAGIGLPTLRGQRRNR"
     gene            5538..5927
                     /locus_tag="VM910_38060"
     CDS             5538..5927
                     /locus_tag="VM910_38060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI68340.1"
                     /translation="MKPIDLEDSVLENWRPGVQTRMLISAQNGANQLCIFEQWVAPDT
                     GAPAHSHPVEEILTVLAGEAEVWMDEARARLAAGQSVIVPAGRRHGFRNSGSGTLHLH
                     AVLASPIFEALPEGASVPVRRWEAVSK"
     gene            6012..7211
                     /locus_tag="VM910_38065"
     CDS             6012..7211
                     /locus_tag="VM910_38065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI68341.1"
                     /translation="MSNIGGLSRARLKRMRDIMAGHVESGRVPGMVTLVSRHGEVHAE
                     AIGKMAIGGAPMQRDTIFRIASMTKPVGAVAAMILVEECRLRLDDPVDAFLPELADRK
                     VLRSIEAEIDDIVPAKRAITLRDVLTFRLGYGMIPIFPDRYPIQKAVSAAGLAPGPVF
                     PCFPPDELMRRYGSLPLVHQPGERWLYNAGSEMLGVLIARAAGQSLGEFLRERIFEPL
                     GMRDTAFFVPQGKRDRFATTYIRDPETQQLKLFDDPVSGKFANPPVFENAGAGLVSTA
                     DDFNAFAQMLLNGGRFGNERILSRPSVELMTTDQLTPEQKLGSEMFFSDNRGWGFGLS
                     VFTRRDHLYNVPGRYGWEGGYGTSWYSDPKESFTGILLTQRLMDSPQAPAVMADFWTS
                     AYQAIDD"
     gene            7347..7763
                     /locus_tag="VM910_38070"
     CDS             7347..7763
                     /locus_tag="VM910_38070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVI68342.1"
                     /translation="MRFMMLMIPTGYEAAAPGTMPPADAVAAMMKYNEALKEAGVLIT
                     LDGLHPPSMGARVSFEGGKPVVTDGPFTESKEVIGGYWMIDVKSREEAIAWAKKCPAS
                     ANEIIEIRQVQEMADFPADVQEAASGFAEMQRRSAG"
     gene            7827..8192
                     /locus_tag="VM910_38075"
     CDS             7827..8192
                     /locus_tag="VM910_38075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68343.1"
                     /translation="MSAKNTFLAVFLGSKTGSKMAAWNALPEGERRAKEQQGIAAWKA
                     WVEKHQGAIAEMGGPLGKTKKVDPRGVADISNQMGAFTVVRADSHEAAAKMFGGHPHF
                     TIFPGEAVEIMPVLPIPGG"
     gene            8287..>8432
                     /locus_tag="VM910_38080"
     CDS             8287..>8432
                     /locus_tag="VM910_38080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI68344.1"
                     /translation="MSWRKDQRRAERGYHHGNLKEALLQAALDLIAQKGAAGFTFADA
                     ARMAG"
BASE COUNT         1591 a         2602 c         2683 g         1556 t
ORIGIN      
        1 tcgatttcct tcctgctccg gctccagccg gcgacacgaa agccgatccg gcgcaaggag
       61 tcggcggcat tggtgcctag cctgcccaac cccatgatgc cgactgaaat cgcgctcgcc
      121 ggccactgga atttcggcgc ccagcgcttc tcgcgttgcg actgccgcaa atagggttcc
      181 tgccggtgat gcatgagaac atgcagcacg acatattcgg tcatgcgatc ggtgagatcg
      241 gcgaccgcga cgcggaccag cggcacgtcc ggcaaggtct tgtcggacat cagcgcatcg
      301 acgccggcac cgagattgaa aatcaccttc agattcggaa aggctgccag ttcgccggga
      361 accggcttcc agactgcggc gtaacgaacc tctgcgggat cgaattcggc cctcggcaac
      421 aggtgcaccg cccgatcggg gcaaacctcg tcgaacctcg ccttccaccg ctccggcgac
      481 cagttgtcgg tgccgccatg gatcaagagc gccagcgcgc ctttcttcat tcgatcgccc
      541 caaattcagc tataggtgca gcggattccg gcaacatgac agaaccggct ctgccgcgca
      601 aaattgtcac ccttgctgtc ggctccgggc atactcgttc gtcttgcaga aaaaggggac
      661 ccctacacat gctctacgcc atactctgct atcacgacga ggatttcgtc ggttcctgga
      721 ccaaggaaca ggacgcggcc gtcatgacca agctggcggc ggtgcaggag aagctccaca
      781 agcagggccg gcttggtccg gtcgcgcgcc tgttgccgac aacggcggca accacgctcc
      841 gcaaggagaa tccgccgctt gtgctcgacg gtccctatgc cgaaaccaag gaacagcttc
      901 tcggcttcta ccttgtcgat tgcaaaaacc tcgacgaggc gctcgatgtc gcccgcgatc
      961 tcggcgcggc caatcccggc ggcgcctatg aaatccgccc ggtcggcatg ttcgcgcccg
     1021 gaggcgcgct gaaatgaccg acgccgcctg gatcgatgcc gcgctgacct cggcgcgtcc
     1081 ccaggccgtc ggtgcgctgc tgcgatattt ccgtaacctc gacaccgcgg aagaggcgtt
     1141 ccagaacgcc tgcctgcgcg ccttgaagag ctggccgcaa aacggccctc cgcgcgatcc
     1201 ggcttcctgg ctgatcatgg tcggccgcaa cgcggcgatc gatgaagtgc gccgcggccg
     1261 caggcaggag ccgttgcccg aggacgaccg gtcgatttcc gatctcgacg acaccgagga
     1321 agcactcgcc gaacggctcg acggttcgca ttaccgcgac gacatcctgc ggctcttgtt
     1381 catctgctgt catcccgatc tgccgcccac acaacagatc gcgcttgcgc tgcgcatcgt
     1441 gtccgggctc acggtcaggc agattgcgcg cgccttcctc gtcaccgagg ccgcgatgga
     1501 gcagcgcatc acccgcgcca aagcccgtgt cgcggatgcc ggcgtgccgt tcgaaacgcc
     1561 cggtgcggtc gaacgcagcc agcgtctcgc ggcggtcgcc gcgatgatct atctgatctt
     1621 caatgaaggt tattcggcga gcggcgacac cgccgacacg cgaaagccgc tctgcgagga
     1681 ggcgatccgg ctggcgcggc tgttgctgcg gctgttccag agcgagcccg agatcatggg
     1741 gctgacggca ctgctgctgc tgcaacatgc ccgcgcagac gcgcggttcg atgccggggg
     1801 cacattgatc ctgctcgagg accaggatcg ttcgaagtgg aatcaaaagc tgatcgcgga
     1861 ggggcttgcg ctgatcgaca aggcgatgcg ccatcggcaa agcggcccct atcagatcca
     1921 ggcggcgatc gcagcgctgc attcccgtgc cgagcggccc gaggataccg actggaagca
     1981 gatcgacctg ctctatggcg cgcttgaatt ggtgcagccg tcgccggtgg tgacgctcaa
     2041 ccgcgcggtt gcggtgtcga aagtgcaggg ggcgcaggct gcactcgaca tgatcgagcc
     2101 gctcgcgccg cggctgtcga actatttcca cttcttcggc gtgcgcggcg cgttcctgat
     2161 ccagctcggc cgcaacgaag aggcgcgcgt ggcgttcgac cgcgcgattg cgctcgccaa
     2221 cacctcggcg gaagcggccc atatccgcat gcatctcgac cgcctgatgc gcgacagcaa
     2281 gccgcgcgag aagaaggcga agactgattg aggcgcatca atgctcctac cctcccctta
     2341 aagggccctt agaggcgggg cagcatcctt tccgcatcgt catggccggg cttgtcccgg
     2401 ccatccacgt cttggcaaat cgaaagaaag gcgtggatgc ccgggtcaag cccgggcatg
     2461 acgagtctgt gggacgactt aagtcgtcac gagtcgcgcc gaaaatccac gattcgagtc
     2521 acggccgtgt cgcgccgaac ttgccgcgcg acctcctctc gttgctgagt tcgcttccac
     2581 cgcagcggaa gtgtgtgctc cgtccccctg caaggcccct ggaggggagg gtaaccagag
     2641 cggcgcactt tccaaaaatt atttccacac tccctgtcgg atcgcgttgt tcccgtccgt
     2701 ctattggtca aacgccaatt cctggggaca agccatgccg acgatgatcg aagcgaagcc
     2761 ggtgtcaaag ccagcgctct tcatgggccg ccttgtcagc ggcctcgtga tcctgttcct
     2821 ccttttcgac ggcgcgatga agctcgtccc gttgcaggtc gtcacggaaa cgatggacag
     2881 ggtgggctac ggttcgagcg aaacgctggc gcgaagcctg ggcctcatca ccatcgtctg
     2941 caccttgctc tattcgtttc cgccgacctc gttcgtaggg gcgatccttt tgaccggcta
     3001 tctcggcggc gcggtggcct cacacgtgag gatcggcagt ccgctgttca cgcacattct
     3061 gttcggcatc tatctcggtg tgatggtgtg gggcggcttg tggttgcggg aggggacggt
     3121 gcgcgcgatg ctgcccttcg accgctgacc cgccgatact ttcaaccaac cgacaacgga
     3181 gcccgccatg tttgaagtca tcatcattat cgccgtgctg cttgcgcttg cgatcgccgc
     3241 gatcctgatg cttgcggccg ccaggccgga tacgttcagc gtacgacgca cggcgaccat
     3301 gaatgcgtct gcggaaaaaa tcttcgcgtt gatctccgat tttcgccaat ggggccgctg
     3361 gtcgccctgg gaaaccaggg atcccggaat gaagcgcact tatgacggcg ccgagcgcgg
     3421 caagggtgcg gtctacgcct gggacggcaa caagaatgtc ggggcgggac gcatggagat
     3481 tctcgaagcc gcgacgccgt cgaaagcgct gatcaagctc gatttcatca agccgttcga
     3541 gggtcataac accgcggaat tcacaatgct gccgcagggc agtggcacca ccgtcacctg
     3601 ggtgatgcaa ggtccggcgc cgttcatggc caaattgatg cacgtgttcg tcaacatgga
     3661 caggatgctc ggcaaggact tcgaggcagg cctcgccaat ctcaagaagc aggtcgagag
     3721 ctgaacgcgg ttctcgatct ctcagcaacc ccgcaagcgt aaaaccgagg agcaaacgat
     3781 gcaggtcaat ccttacgtgt actatgacgg caactgcgag gcagccttca aattctacga
     3841 aaaggagctg ggcgccagga tcgagatgat gctgcgctac gaggaggcgc ctgcggacat
     3901 gccgtcatcc ccggagcaca agaagaagat tatgcatgcg cggatctcga tcgatggcgc
     3961 caccatcatg ggttccgacg cgcccccgaa gcattttcac aagccgcagg gcttctcgct
     4021 gtcgctcacg gtcgacgatc ctgcggaggc cgaacgcaag ttcaacgcgc tttgcaaggg
     4081 cggcgccgtc aacatggcgt ggtcgaaaac gttcttcgca aaggggttcg gcatgggcgt
     4141 cgaccagttc ggcattccct ggatggtgat aagcccgctg ccgaattcct gaaagtcatc
     4201 ggcaggtgcg gtacatcgca gcgagttcgc gcccacgcag caagagcagc cgcgacgtca
     4261 atccgccgcg ctggacgatc cagctccgga tcgggtcggg ataggcctcc agcaccacct
     4321 tggaggcctc cggctcggca tatagccgcc cgcggcggtc catcgatcgc gccgcatgga
     4381 agccgaagac cgcgcggcgc gtgacgcaaa tacggtcgct cggcaccatg ctcagcacca
     4441 gcgtgcaggc cgacagacag ggaccgtcga tcaccacgcg ttcgccggat cggttcacct
     4501 cttcgaacag atcgaggaat gggccgacct gaccgccggg acttgagaga atgcgcacgt
     4561 cggcgcgcgc cagcagcgtc gcgaaaaacc acaacgcgat cgcaagaccg acgacgaaaa
     4621 ttatgcgcgt gatccccatc gtttcacctg tttcgtcgtt ggccgcgcag cgtcggcaag
     4681 ccgatgccgg ccgcatcgaa gccgccatcg accgccagga tttgtccggt gatgtagctg
     4741 gagcggtcgc agctcagaaa gtagatggct tccgccaact cttcttcaag gccgtaacgg
     4801 ttgagcggaa tcgcatcgtg atagtcggcg cggatctcgg gagtgtgcac ctcttttgcc
     4861 atcgcggtgt cgaccggacc gggcgccacg ccgttgacgc ggatgccgga agaggcaagt
     4921 tccaccgcga gctgcttggt cagatgcgcg agcgccgcct tgctggtgcc ataggccgag
     4981 cgcagcgtgg aagcacgtac cgccgagatc gaggtgatgt tgacgatcgc gccgccgccc
     5041 ccttcgcgca gcagcggcac cgctgccttg gcaaacagga aaggaccggt gagattaacg
     5101 gcgaggatcc ggttccagtc ggcgtcagcg gtctccatga gcggcgcgaa cacggctacg
     5161 ccggcattgt tgatgagtgc gtcgagcctg ccgaagcgtt tgcgcagcgc atcgattgcg
     5221 gcctcgaccg cggcggcgtc ggacacgtcg cagtgaagcg ggagcgtgtt gtcgggatcg
     5281 gcgagcccgt cgactgctcc gcgcaacaat tcgtcctcga tgtcgagcag cgcgacgcgc
     5341 cagccttcgg cgagaaatcg cttcgcggtc gcaagtccga tgccgcgcgc agcccctgtg
     5401 acgagcgcga cattttgcgg ggaggctgcc atggtcgatc cgtgcttcct ggatggtcgt
     5461 ggtttgctcc actagcacga aagcgcatgc tttgctttgc tcgcttgtca gcatctgcca
     5521 aagggagggc ctttcccgtg aaaccgatcg atctcgagga cagtgtgctg gaaaattggc
     5581 gtccgggagt tcagactcgc atgctgattt cggcgcagaa cggtgcgaac cagctctgca
     5641 tcttcgagca atgggtcgcg cccgataccg gcgcgcccgc ccacagccat ccggtcgagg
     5701 aaatcctgac cgtgctcgcg ggcgaagccg aggtctggat ggacgaggcg cgagcgaggc
     5761 ttgcggccgg gcaatcggtg atcgtgccgg cgggtcgccg tcacgggttc cgcaacagcg
     5821 gtagcgggac tttgcacctg catgcggtgc tggcttcacc gatttttgaa gcgctgcccg
     5881 aaggtgcctc cgtgccggtc cggcggtggg aggcggtttc gaaataaatt tcgcgggcca
     5941 tgtcgtccgt ggccggtctc gttcgtctaa tcaggggctg gtgtcgttcg gaaggaatga
     6001 acatgcatgg aatgagcaac attggcggcc tgtccagagc gcgcttgaag cggatgcgcg
     6061 acatcatggc cggccacgtc gagagcggtc gcgtgcccgg catggtaacg ctggtgagcc
     6121 gccatggcga agttcacgcc gaggcgatcg gtaaaatggc catcggcggt gcaccgatgc
     6181 agcgcgatac catcttccgc attgcctcga tgaccaagcc ggttggcgcg gtggctgcaa
     6241 tgattctggt cgaggaatgc aggttgcggc tcgacgatcc cgttgatgct tttctgccgg
     6301 aacttgccga ccgcaaggtg ctgcgttcga tcgaagccga gatcgacgac atcgtaccgg
     6361 caaagcgcgc gatcacgctg cgcgacgtgc tcacctttcg cctcggctat ggcatgatcc
     6421 cgatctttcc ggaccgctat ccgatccaga aggcggtctc ggccgcgggg ctcgcgcccg
     6481 gccccgtatt tccctgcttt ccgccggacg aattgatgcg gcgctatggt agcctgccgt
     6541 tggtgcatca gcccggcgag cgctggctct acaacgccgg atcggagatg ctgggcgtcc
     6601 tgatcgcgcg cgctgcaggc caaagcctcg gcgaattctt gcgcgagcgc atcttcgaac
     6661 cgcttggcat gagggacacc gcattcttcg tgccgcaggg aaaacgcgat cgctttgcga
     6721 ctacctacat ccgcgacccc gagacgcagc agttgaagct ctttgacgat cccgtgagcg
     6781 gcaagtttgc aaacccgccg gtgttcgaga atgccggcgc aggcctcgtc tcgactgcgg
     6841 atgatttcaa cgcctttgca cagatgttgt tgaacggtgg gcggtttggc aacgagcgca
     6901 ttctgtcgcg gccttcagtt gagctgatga cgacggatca gctcacgccg gagcagaaac
     6961 tgggctcgga aatgttcttc agcgacaacc gcggctgggg ctttggtctc tcggtgttca
     7021 cccggcgcga ccatctctac aacgtgcccg gccgctatgg ctgggaagga ggttacggca
     7081 cctcctggta ttcggacccc aaggagagtt tcacggggat cctgttgacc cagcggctga
     7141 tggattcacc gcaggcgccg gctgtgatgg cggatttctg gacctcggcc tatcaggcga
     7201 tcgatgactg aggcggagcg cgtgaaacaa ttaccttgca aataattccg ctcgcgctgt
     7261 cggttgggca aaaccttctt cgtcttcttg acgagtgccg attgcgagcg ggcgccaccg
     7321 agacgagaac cgaacggaga gacatgatgc gattcatgat gttgatgatc cccacgggct
     7381 atgaggcggc ggcgcccggc accatgccgc cggcagacgc ggtcgctgcg atgatgaaat
     7441 acaacgaggc gctgaaggag gcgggcgttc tgatcacgct cgacggtctc catccgccgt
     7501 cgatgggcgc gcgcgtttcc ttcgagggcg gcaagccggt cgtgaccgac ggccccttca
     7561 ctgagagcaa ggaagtgatc ggcggttact ggatgatcga tgtgaaatcg cgcgaggagg
     7621 cgatcgcctg ggccaagaaa tgtccggcct ccgcgaacga gatcatcgag atccgtcagg
     7681 tgcaggaaat ggctgatttc ccggccgacg tgcaggaggc agccagcgga tttgccgaga
     7741 tgcagcgccg ctccgccggc taggcgcgga agcggtctgc accagcattc gacgttcttt
     7801 caaccagcaa aaggagacaa catccgatga gtgcgaagaa cacctttctc gccgtctttc
     7861 tcggcagcaa aaccggttcg aaaatggcag cctggaacgc gcttcctgag ggagaacggc
     7921 gcgccaagga acaacagggc atcgctgcgt ggaaggcgtg ggtcgagaaa caccaaggtg
     7981 ccatcgccga gatgggtgga ccgctgggca agaccaagaa ggtcgatccg cgcggcgttg
     8041 ccgatatcag caatcagatg ggtgccttta cggtcgttcg ggccgattcg catgaggcgg
     8101 ccgcgaagat gttcggaggg catccgcact tcacgatctt tccgggcgaa gccgtggaga
     8161 tcatgccggt tctgccgatt ccgggcggct aggcgctgtt tcagcccgtt tacgcttgcc
     8221 atttccggcc ttcaggaggg ctttaagtag tgacctctgg agccagctca tgtaaaaccc
     8281 ttttacatga gctggcgaaa ggatcagcgc cgcgccgagc gcggctatca tcacggcaat
     8341 ctgaaagagg cgttgctgca ggcagccctc gatttgatcg cgcagaaggg ggccgccggc
     8401 ttcaccttcg cggacgctgc gcgcatggcg gg
//
LOCUS       DATLJT010001671         8618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1784369,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001671 DATLJT010000000
VERSION     DATLJT010001671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784369"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VM910_38085"
     CDS             complement(<1..916)
                     /locus_tag="VM910_38085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68345.1"
                     /translation="MASIVRARFRLLSSLVFALCALLAAQHPARAQTKYPERSVRIVL
                     PFAAGGVADITARIVAEKLSEKLAQRFYVENQAGAGGIVAARTVTSSPPDGYTLALLS
                     NGTAVSVSLFRKLPFDPLKDFAPISSLGFFDFIFAKGANSPFKSLADVIAAAKAKPGT
                     LNVGTINVGSTQNLSAELFKTAAGIDFAIIPYRGTPEIQVALLQGDVALTIDSYSAMK
                     GNLADGKLRALASSGAQRSEATPELASVGESGVADYDVVSWNALFAPAGTPPEIVGTL
                     NAALRDILADPDVKKRLLELGIEAKASTP"
     gene            complement(975..2534)
                     /locus_tag="VM910_38090"
     CDS             complement(975..2534)
                     /locus_tag="VM910_38090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HVI68346.1"
                     /translation="MSLLSDPRWGEAATHLPDYLGNHVRVSVAALVLGLIVSLPLAIV
                     SRDRPLIRGILLGFASVVQTVPGLALLALFYPLLLAAASLTLSWFGFGFSAFGFLPAV
                     LALALYSMLPVLRNTVTGLRGIDADILQAAQAVGMTPQQSLFMVELPLALPVIMAGIR
                     TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVFVLFGCLAAAVLALAVDQLLALV
                     ESGVRSRSRLRTGLGGLGIAALVAATLVPSLARSHSSYIVGAKTFTEQYVLSALIAER
                     LRAEGLSASSREGLGSNVIFDALASNDIDVYVDYSGTLWVNQFHRTDMLPRQELLTEL
                     KAILAKQNITLFGELGFENAYALVMPKKRAEELGIRSIADLASRAPTLSIAGDYEFFS
                     RPEWAALQRAYGLSFRTQRTMQPDFMYAAVASGEVDVIAGYTSDGLIRKYDLVVLDDT
                     RHAIPPYDAILLLAPKRAGDEALQAALKPLLGRINIEDMREANLRASGNDASASPAAV
                     ARWLWERIGRR"
     gene            complement(2531..3328)
                     /locus_tag="VM910_38095"
     CDS             complement(2531..3328)
                     /locus_tag="VM910_38095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI68347.1"
                     /translation="MTTPLIAYTNVSKSFAGGRSGERVVAVDDVSLDVSEGEFLAIVG
                     GSGSGKTTLLRLANRLIEADRGRITIAGDEVGAVDPVQLRRRIGYVSQSGGLFPHLTV
                     ADNIGITPKLLGTPPAEVSARIDELLALVRLDGGAHRDRLPQELSGGQRQRVGVARAL
                     AAHPRIVLMDEPFGALDPLTRDALGEDYRALHGKLSVTTIMITHDMTEALLLADRVAV
                     MRAGRLLSLGTPDELSRSGDAYVSELLRTPRRQAERLNTLLPRDGAA"
     gene            3471..4286
                     /locus_tag="VM910_38100"
     CDS             3471..4286
                     /locus_tag="VM910_38100"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68348.1"
                     /translation="MDDLSAQAVALDDATVAFRLSDTRTYTAVEKASLLVAHGEFVAI
                     VGPTGCGKSTLLNVAAGLLRPASGSVKIFNRPLAGLNHDAGYLFQADALFPWKTALDN
                     VAIGLEIAGVRRAEALSRAQDFLASVGLGAFAARYPHMLSGGQRKRVGLAQVLIRHPK
                     ILLMDEPFGPLDAQTRQIMGNLLLDLWSADRKAVLFVTHDLEEAIALADRVVIMSAGP
                     GARIIGDWRVPLARPRDIFEVRLDKEFHALHREIWGVLKAEVMKGYAQSTGGA"
     gene            4289..5077
                     /locus_tag="VM910_38105"
     CDS             4289..5077
                     /locus_tag="VM910_38105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68349.1"
                     /translation="MSRIGLLSLQLLVAVVALVLWQFFSTVPVFGKVWLPPFFFSTPI
                     DVFSQVIKWFSTGVIWKHLAITLWESILAFVLGSAAGVAVGFWFARQPRVAAVFDPYV
                     KMINALPRVVLAPIFTLWLGLGIWSKVALGVTLVFFIVFFNVYQGVKEVSQTVLDNGR
                     MLGMNERQLTRHVYWPSALSWMFSSLHTSVGFAVVGAVVGEYLGSAAGLGYLIQQAEG
                     VFDVAGVFAGMFVLSLFVVLIDMVVTLVERRLLVWRPQAAETQA"
     gene            5205..6233
                     /locus_tag="VM910_38110"
     CDS             5205..6233
                     /locus_tag="VM910_38110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68350.1"
                     /translation="MKKMLSKLAGALLAMTLASPLAAAESKVTIAVGGGACLCYLPTL
                     LAKQLGEFEKAGVAVDLVDLKGGSDALKAVLGGSADVVSGYFDHCVNLAARKQELVAF
                     VVYDRYPGLVLVVSPSHTSEINSVKDLAGKKVGVSAPGSSTDFFLKYLLKKNGLDPTS
                     AAVIGVGLGATAVAAMEQGQIDAAVMLDPSVTVLQGNHADLKILADTRTQKDTLATFG
                     GEYPGGALYSTAAWVSSHEKEVQALTNAIVSTLAWIHSHTAEEIMAKMPEETVGKDKA
                     LYLAALKNTIPMYSETGKMDPKGADAVLAVFSEGSPEVAKANIDVSKTFTNKYVDQAK
                     KTTGMNAK"
     gene            6285..6530
                     /locus_tag="VM910_38115"
     CDS             6285..6530
                     /locus_tag="VM910_38115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68351.1"
                     /translation="MKRREFLSFAVGAAIGLPLAANAQSGRVRRIGLMANLPLRPIER
                     FRDRMKQLGYVEGQNLVVEYRFAEGRDELYTGFAEEL"
     gene            6532..7248
                     /locus_tag="VM910_38120"
     CDS             6532..7248
                     /locus_tag="VM910_38120"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68352.1"
                     /translation="MPVELIVAWGTPAAFAAKRATSKIPVVITVGDVINTGLVSNLAR
                     PDANITGFVALNVELEEKRLELIKEIVPHLSKVAVLGNRANPLNKINSEAVRRVAERL
                     SVSIEVVNIVRSDEVEAALGAIASVHPDAVIIASDTLLLSERARITKFMADNGVPAVY
                     PFREYADAGGFIFYGANIATLFERAADYVHRILNGESPQNLPIQQATSFEMIINLKAA
                     AKLGLTLPPAVLARADEVVE"
     gene            7347..8048
                     /locus_tag="VM910_38125"
     CDS             7347..8048
                     /locus_tag="VM910_38125"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVI68353.1"
                     /translation="MDPTIHRVTMLDLKVESSVWPFASTRRGEIDAHFADRQRERPNM
                     WNGRVLLGRDPVFEGPRFSARYFETDFASFLSWRDWGFPDSAVFNGFGMGALRTSDGA
                     FILGEMSAQTANAGRIYFPAGTPDPNDIAGGAVDIAGSVTREIEEETGLTAADYSASP
                     HWDCIVVGPSIAMIRLLDLAMTAEAARARIEASLARQAHPELSAIHLVRSLRDLSPQM
                     PRFVTAFIERELGTS"
     gene            8193..>8618
                     /locus_tag="VM910_38130"
     CDS             8193..>8618
                     /locus_tag="VM910_38130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68354.1"
                     /translation="MLTGKVVRLFFGLMVALATSGVAISAHAQEKKIKIGVIFDLTGP
                     LAGGGSELEYLGAKIMLDQFAKTGVEGYKVEAIYADAQSKPDVAINESVRLLEQEKVD
                     MLLGFFSSAQCVPVADRVEQLKKFMWMTTCISSAVFANKN"
BASE COUNT         1584 a         2674 c         2749 g         1611 t
ORIGIN      
        1 gcggtgtgct ggctttcgcc tcgatgccga gttcgagcag ccgcttcttc acgtcaggat
       61 cggcaaggat atcgcgcagc gcggcattga gcgtgccgac gatctcgggc ggcgtgccgg
      121 caggcgcaaa caacgcgttc cacgacacca cgtcgtaatc ggcaacgccg ctttccccaa
      181 cgctcgcaag ctccggcgtt gcctcggaac gctgcgcgcc ggaggaggcg agtgcccgca
      241 gcttgccgtc ggcgaggttg cccttcattg cggaatagct gtcgatcgtc agcgcgacgt
      301 cgccctgcaa aagcgctacc tggatttccg gcgtgccccg atagggaatg atcgcaaagt
      361 cgatgccggc agcggtcttg aacaattccg ccgagagatt ttgcgtgctg ccgacattga
      421 tggtcccgac attcagcgtc ccgggttttg ccttggcggc ggcgatgaca tcggcgagcg
      481 acttgaacgg ggagttcgcg cccttcgcga agatgaaatc gaagaagccg aggctcgaga
      541 tcggcgcaaa atccttcagc ggatcgaaag gcaatttcct gaacagcgac acgctcacag
      601 cggtgccgtt cgagagcagt gcgagcgtat agccgtccgg cggcgaggag gtgaccgttc
      661 gcgccgcaac gatgccgccg gcgcctgcct ggttctcgac atagaagcgc tgcgcgagct
      721 tttcggacag cttttcggcg acgatgcgcg cggtgatgtc ggcgacgcct ccggcggcga
      781 acggaagcac gatgcgcacc gaacgctccg gatatttcgt ctgcgcgcgg gccgggtgct
      841 gcgcagccag gagcgcacac agggcaaata ccaacgagga caggagacgg aagcgcgcgc
      901 ggacaataga agccatggac gacctttttt tggcttatag cacgcgagcg cgccgatcca
      961 agccttggtg gcgcctaacg cctgccgatc ctctcccaca gccagcgcgc caccgcagcg
     1021 ggggaggcgg aggcgtcatt gccactggcg cgcagattgg cctcgcgcat atcttcgata
     1081 ttaatgcgcc cgagcagggg ttttagtgct gcctgcaagg cctcgtcgcc ggcgcgcttc
     1141 ggcgccagca gcagaatggc atcgtagggc gggatcgcgt gtctggtatc gtcaagcacc
     1201 accagatcgt atttcctgat caggccgtcg ctggtgtagc ccgcgatcac gtcgacttcg
     1261 ccggacgcca cggcggcgta catgaagtcc ggctgcatcg tgcgctgcgt gcgaaaggaa
     1321 agcccgtaag ccctttgcag cgccgcccat tccggtcgcg aaaagaactc gtagtcgccg
     1381 gcgatcgaca aggtcggcgc gcgcgacgcg agatcggcga tcgaacggat acccagctct
     1441 tccgcgcgct tcttcggcat gaccagcgca taagcgttct cgaagccgag ctcgccgaac
     1501 agcgtgatgt tctgctttgc caggattgcc tttagttcgg tcaacagctc ttgccgcggc
     1561 agcatatcgg ttcggtgaaa ctggttgacc cagagagtac cggaatagtc gacgtagacg
     1621 tcgatgtcgt tcgaggcgag cgcatcgaag atcacgttgg agccgagccc ttcgcgcgag
     1681 cttgccgata gtccctccgc gcgcagccgc tcggcgatga gtgccgacag cacatattgt
     1741 tctgtgaagg tttttgcgcc gacgatatag gatgaatgcg agcgggccag cgaaggaacg
     1801 agtgttgccg caacgagcgc ggcgatgccg aggccgccga gacctgtgcg cagacggctt
     1861 cggctgcgaa ccccgctctc gaccagcgca agcagttgat cgactgcaag cgcgagcacg
     1921 gcggcggcca ggcagccgaa cagcacgaag acccagttct gcgtttgcag cccggcaaag
     1981 atataattgc cgagcgaggt ctggccgatc ggtgtcgaca acgtcgcggt tccgatcacc
     2041 cagactgccg cggtgcggat gccggccatg atcaccggca gtgccagcgg caattccacc
     2101 atgaacaacg attgttgcgg cgtcatgccg acggcttggg ccgcctgcaa gatgtccgcg
     2161 tcgatgccgc gcagcccggt cacggtgttg cgcaataccg gcagcatcga atagagcgca
     2221 agcgccagca ccgccggcag gaagccgaag gcggagaagc cgaagccgaa ccagctcagc
     2281 gtgagcgatg cggcggcgag caggagcgga tagaacaagg cgagcaacgc aagccccggc
     2341 acggtctgca ccacgctggc aaagccgagc aggatgccgc ggatcagggg ccggtcgcgc
     2401 gagacaatcg caagcggcag gctgacgatc agccccagca ccagcgccgc aacgctgacc
     2461 cgcacatggt tgccgagata atcgggaagg tgcgtcgctg cttcgcccca gcgcggatcg
     2521 gacaaaagac tcatgccgcg ccgtcccgtg gaagcagcgt gttcagccgc tcggcctgcc
     2581 ggcgcggtgt gcgcaggagt tcgctgacat aggcgtcgcc gctcctggaa agttcgtccg
     2641 gcgtgcccaa cgacaataac cgccccgcgc gcatcaccgc gacgcggtcg gcgagcaaca
     2701 gcgcttccgt catgtcatgg gtgatcatga ttgtggtgac cgagagcttc ccgtgcagcg
     2761 cgcggtagtc ctcaccgagc gcgtcgcggg tcaagggatc gagcgcgccg aacggttcgt
     2821 ccatcagcac gatgcgggga tgtgcggcga gcgcccgcgc cactccgacg cgttgccttt
     2881 gtccgcccga taattcctgc ggaagccggt cgcgatgcgc gccgccgtca agccgcacca
     2941 gtgcgagcaa ttcgtcgatc cgcgccgaga cttccgccgg cggcgtcccc aacagctttg
     3001 gcgtgatgcc gatattgtcg gcaaccgtca gatgcggaaa cagccctccg ctctgggaga
     3061 cgtaaccgat acgccgccgc agttgcaccg gatcgaccgc gcccacctca tcgcccgcga
     3121 tggtgatgcg gccgcggtcg gcctcgatca gccggttggc gagccgcagg agcgtggtct
     3181 tgccggagcc ggagccgccg acgatcgcga gaaattcgcc ctcactgaca tcaagcgaga
     3241 cgtcgtcgac cgcgacgacc cgttccccgc tgcggccgcc ggcaaaactc ttgctgacat
     3301 tggtgtaggc gatcagcggc gtggtcatgc ccctcggacc gggattgaag cggcgagcga
     3361 tttcaccctc cttcgcgggg agggtaagta ccaactacca cgccgggccc tttgattgaa
     3421 cttgtccgcg caagcggcta agccatcagc caaaaccggg agggaaggca gtggacgatt
     3481 tgtcggcaca ggcggtcgcg ctggatgacg cgacggtggc cttccgcctg tccgatacgc
     3541 gtacctacac cgcggttgaa aaggcgagcc tgttggttgc ccacggcgag ttcgtcgcca
     3601 tcgtcgggcc gacggggtgc ggaaagtcga cgctcctcaa cgttgcggcc ggcctgctgc
     3661 ggcctgcctc cggctcggtc aaaatcttca accggccgct cgccgggctc aaccacgacg
     3721 ccggctacct gtttcaggcc gatgcgctgt ttccgtggaa aaccgcgctc gacaatgtcg
     3781 caatcgggct cgaaattgcc ggcgtgcggc gggccgaggc gctctcccgc gcgcaggact
     3841 tccttgcttc ggtcggcctc ggcgccttcg ccgcccgcta tccgcacatg ctgtccggcg
     3901 gtcagcgcaa gcgcgtcggt ctggcgcaag ttctgatccg gcatcccaaa atactcctga
     3961 tggacgagcc gttcggcccg ctcgatgccc agacccggca gatcatgggc aacctgttgc
     4021 tcgacctctg gagcgccgac cgcaaggcgg tgctgttcgt cacccatgac ctcgaggagg
     4081 cgatcgcact cgcagaccgc gtcgtcatca tgtcggcggg gcccggcgcg cgcatcatcg
     4141 gcgactggcg cgttccgctc gcgcgcccgc gcgatatttt cgaggtgcgg ctggacaagg
     4201 aatttcacgc gctgcatcgc gagatctggg gcgtgctcaa ggctgaggtg atgaagggct
     4261 acgcgcagtc gacgggaggc gcttgagcat gtcgcggatc ggccttctct cgctgcaact
     4321 gcttgtcgcc gtcgtggccc tcgtgctgtg gcagtttttc tcgaccgtgc cggtgttcgg
     4381 aaaggtgtgg ctgccgccgt ttttcttctc gacgccgatc gatgtgttca gtcaggtcat
     4441 caaatggttt tcaaccggcg tgatctggaa gcatctcgcg atcacccttt gggaatcgat
     4501 cctcgccttc gtgttgggtt cggccgccgg cgtcgcggtc gggttctggt tcgcgcgcca
     4561 gccccgcgtc gcggccgtgt tcgaccctta cgtgaaaatg atcaatgcgc tgccccgcgt
     4621 ggtgctggca ccgatcttca cgctgtggct cggcctcggc atctggtcga aggtcgcgct
     4681 cggcgtgacg ctcgtgtttt tcatcgtgtt tttcaatgtc tatcagggcg tcaaggaagt
     4741 gagccagacg gtgctcgaca acggccgcat gctcggcatg aacgagcggc aattgacacg
     4801 gcatgtctac tggccttcgg cgttgtcgtg gatgttctca tcgctgcata cctcggtcgg
     4861 ctttgccgtg gtcggcgccg tggtcggcga atatctcggt tccgcggcgg gtctcggata
     4921 tctgattcaa caggccgaag gtgtgttcga cgttgccggc gtgtttgccg gcatgttcgt
     4981 gctctcgctg ttcgtcgttt tgatcgacat ggtggtgacg ctggtcgaac gacggctttt
     5041 ggtgtggcgg ccgcaggcgg ccgagacaca ggcttagact ttcgtcgtca tttgcgcgcc
     5101 aacgatgatg tttggcagcg ctccggtccg ccgggcgctg gcgacgcagc taccttctca
     5161 agccctccgc gctgctttat cgtgccggca agatggagga aaccatgaaa aaaatgctca
     5221 gcaaactggc gggcgcgctg cttgccatga cccttgcttc gcctctggcg gccgccgaaa
     5281 gcaaggtgac gatcgcagtc ggcggcggcg cctgcctctg ctacctgccg accctgcttg
     5341 ccaaacagct tggcgaattc gagaaagcgg gcgttgccgt cgacctcgtc gatctcaagg
     5401 gcggctcgga tgcgctcaaa gccgtgctcg gcggcagcgc cgatgtggtc tccggctatt
     5461 tcgaccattg cgtcaatctg gcggccagga agcaggagct cgtcgctttc gtggtctatg
     5521 accgctatcc ggggctggtg ctggtcgtgt cgccctcgca cacgagcgag atcaattcgg
     5581 tcaaggacct tgccggtaag aaggtcggcg tcagcgcgcc ggggtcttcc accgatttct
     5641 tcctcaaata cctcttgaag aagaacgggc tcgatccaac cagcgctgcc gtgatcggcg
     5701 ttggcctggg cgcgaccgcc gttgcggcga tggagcaggg gcagatcgac gccgccgtga
     5761 tgctcgatcc gtcggtgacc gtgctgcagg gcaaccatgc cgatctaaaa atcctcgccg
     5821 atacccgcac gcagaaggat acgcttgcca ccttcggcgg cgaatatccg ggcggcgcgc
     5881 tctactcgac cgcagcctgg gtctcgtccc acgagaagga ggtgcaggct ctgaccaatg
     5941 cgatcgtctc cacgctcgcc tggatccatt cgcacacggc cgaagagatc atggcgaaga
     6001 tgccggagga gacggtcggc aaggacaagg cgctctatct cgccgcgctg aagaacacga
     6061 tcccgatgta ttcggagacc ggcaagatgg accccaaggg cgcagacgcc gtgctcgcgg
     6121 tgttcagcga gggctcgcct gaggtcgcca aggccaatat cgacgtcagc aagacgttca
     6181 ccaacaaata tgtcgatcag gcgaagaaga cgacgggcat gaatgcgaaa tagcgcgcaa
     6241 gcagattcga gcggcaattc gccggttcgc tccgggaggt gcggatgaag cggcgcgaat
     6301 tcctgagttt tgccgtcggc gccgcaattg gcctgccgct cgcagcgaat gctcaatccg
     6361 gtcgcgttcg ccgtatcggc ttaatggcca atctgccgct gcggccgatc gagcgcttcc
     6421 gcgaccggat gaaacaactg ggctatgtag aaggccagaa tctcgtcgtc gagtaccgtt
     6481 ttgccgaagg cagggacgag ctttatacgg gctttgccga agaactttag catgcctgtc
     6541 gaactgatcg tggcgtgggg aacgccggcg gctttcgcgg ccaaacgcgc cacttcgaag
     6601 attccggtcg tgataaccgt tggcgacgtc atcaacactg gactggtttc caacctcgcg
     6661 cgtcccgatg ccaatatcac cgggttcgtc gccctcaacg tggagctcga ggagaaacgg
     6721 ctcgaactca tcaaagaaat agtgccgcac ctttcgaagg tcgccgtcct gggaaatcgc
     6781 gccaacccgc tcaacaagat caattcggag gcggtacgcc gtgtcgcgga gcggctctcg
     6841 gtctcgatcg aggtagtcaa tatcgtcaga agtgatgagg tcgaggccgc acttggcgcg
     6901 atagcctcag tgcatcctga tgccgtgatc atcgcatccg atacattgtt gttgagcgaa
     6961 cgcgcaagga taacgaagtt catggcggac aacggcgtcc ccgccgtcta tccgtttcgc
     7021 gaatacgccg atgccggcgg cttcatcttt tacggagcca acatcgctac gttgttcgag
     7081 cgcgctgccg actacgtgca cagaatattg aacggcgaaa gtccgcagaa ccttccgatt
     7141 caacaggcaa cgagcttcga gatgataatc aatctgaagg ccgcggcaaa gcttggcctg
     7201 acgcttccgc ccgcggtgct cgcccgcgcc gatgaggtcg tcgaatagaa cccgcttgtg
     7261 accttttcac acgtccaggc gcctttactt tccgcgcgaa agagtgtgct tcgacggcct
     7321 gcgacctccg gagggtacgg actgagatgg atccgaccat tcaccgcgtc acgatgcttg
     7381 acctgaaggt cgagtcctcc gtctggccgt tcgcgagcac gcgacgtggc gagatcgacg
     7441 cgcattttgc cgacaggcag cgcgagcggc cgaacatgtg gaacggccgc gtgctgctcg
     7501 gccgcgatcc cgtcttcgag ggcccgcgct tctcggcgcg ctatttcgag accgattttg
     7561 caagcttcct gtcgtggcgc gattggggct ttcccgattc cgccgtgttc aacgggttcg
     7621 gcatgggcgc gctgcgcact tccgacggcg ccttcatcct gggcgagatg agtgcgcaaa
     7681 cggcgaatgc cggccgcatc tattttccgg ccggcacgcc ggatccgaac gacattgcag
     7741 gcggtgcagt cgatatcgcc ggcagcgtga cgcgcgagat cgaggaggag accgggctca
     7801 ccgccgccga ctacagcgct tcaccgcact gggattgcat cgtcgtcggc ccctcgattg
     7861 cgatgatcag gctgctcgat ctcgccatga cggcggaagc ggcgcgcgcc aggatcgagg
     7921 ccagtctcgc gcgccaggcg caccccgaat tgtcagcgat ccatctggtg cgcagcctgc
     7981 gcgacctcag cccgcagatg ccgcgctttg tgaccgcatt catcgagcgc gaactgggta
     8041 cgtcttagcg acccgccttt agcaaaacgt tcaaggtccg gatcgaggaa agcagcgcta
     8101 ttcggcttga catcgctcgg cctgatcaat gtgatgggct ccaacaagat caagaaaatg
     8161 cgatgcacaa ttcttcgagg gaggaaggtt cgatgctgac cggcaaggtt gttcgcctgt
     8221 tttttgggct gatggtcgcg cttgcgactt cgggcgtggc gatttccgcc catgcccagg
     8281 agaaaaaaat caagatcggc gtgatcttcg acctgaccgg cccgctggcc ggcggcggct
     8341 ccgagctcga atatctcggc gccaagatca tgctcgatca attcgccaaa accggggtcg
     8401 agggctacaa ggtcgaggcg atctatgcgg atgcccagag caagcccgac gtcgccatca
     8461 acgaatccgt gcgtctactc gagcaggaaa aggtcgacat gttgctcggc ttcttctcgt
     8521 cggcgcaatg cgtgccggtc gccgatcgcg tcgagcagct caagaagttc atgtggatga
     8581 ccacgtgcat ttcttccgcc gtgttcgcca acaagaat
//
LOCUS       DATLJT010001672         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1324463,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001672 DATLJT010000000
VERSION     DATLJT010001672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324463"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VM910_38135"
     CDS             complement(<1..467)
                     /locus_tag="VM910_38135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI68355.1"
                     /translation="MTDLVDISGLNIRFTGERTVYAVNGLSLSLGDGEVLGLLGESGS
                     GKSVTLRALMRLLPRKRTEITGSVKVLGRNVLAMSEEQLADFRGQTVSMIFQEPALAL
                     DPIYTIGRQIAETVMRHERKSYSEARARALQMLEVVRIPSAKRRLDSYPHEMSG"
     gene            complement(464..1876)
                     /locus_tag="VM910_38140"
     CDS             complement(464..1876)
                     /locus_tag="VM910_38140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVI68356.1"
                     /translation="MSTEPALMSLTAVAKAIAEKRLSSHEVTRSCLHRIAEWQPKLNA
                     FMTIEAEAALKAAEEADAALARGQSRGPLHGVPLAHKDMYYDAGKIVTCGSAIRRDFV
                     PSVTSTALQRLKDAGQIRLGSLQMVEFAYGPTGHNVHYGAVRNPFARDHITGGSSSGS
                     GSAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTYGRISRAGAMPLSQSLDTVGPL
                     ARTAEDCALLLGLMAGADPADPTASTAAVPNYMAATSGSVKGLKIGVPTAFYVDDLDP
                     EVAKALDDTIAVLTREGAEIVKVELPDQRQLAAACQLVLATEAAAYHKRWLIERPQDY
                     GPQVLMRLQNGLAIPGVSYLEAMRWRGPALAAHDAATSRVDAVLTPVAPVAAPTIAES
                     DVGNSPGAEAVIQRLTRFTRPINYLGLPSLAVPAGFTGKGLPVGMQLIGRSFDEATVL
                     RIGAAFQRATDFHEIVPKLE"
BASE COUNT          362 a          670 c          667 g          333 t
ORIGIN      
        1 ccggacattt cgtgcggata ggaatcgagc cgccttttgg ccgagggaat ccgcaccacc
       61 tccagcatct ggagggcgcg cgcccgcgcc tcggaatagc tcttgcgctc atgacgcatc
      121 acggtctcgg cgatctggcg gccgatggtg tagatcggat cgagcgcgag cgccggttcc
      181 tggaaaatca tcgacaccgt ctggccgcgg aaatcagcca attgctcttc gctcatcgcc
      241 agtacgttgc ggccgagcac cttgacgctg cccgtgatct ccgtccgttt cctcggcaac
      301 agccgcatca acgcgcgcag cgtcacgctc ttgcccgagc cggattctcc gagcagcccg
      361 agcacctcgc catcgcccaa agacagcgag agaccgttga cggcatagac cgtgcgctcg
      421 ccggtgaagc ggatattgag gccggagatg tcgacaaggt cggtcattcg agctttggca
      481 caatctcgtg gaaatcggtg gcccgctgga acgcggcgcc gatccgaagc acggtcgctt
      541 cgtcgaagga gcggccgatc aattgcatgc cgaccggaag acccttgccg gtgaaccccg
      601 ccggcaccgc gagcgacggc agtccgagat aattgatcgg acgggtgaac cgcgtcagcc
      661 gctggatcac ggcttcggca cccgggctgt tgccgacgtc gctttcggcg atggtcggcg
      721 cggcgaccgg cgcgaccggc gtcagcaccg catcgacacg cgaagtcgca gcgtcatgcg
      781 cggccaaagc cggtccgcgc cagcgcatgg cttcgagata ggagacgccg gggatcgcaa
      841 gcccgttctg cagccgcatc agtacttgcg ggccgtaatc ctgcggccgc tcgatcagcc
      901 agcgcttgtg ataggccgcc gcttcggtcg cgagcaccaa ttggcaggcc gctgcaagct
      961 gccgctggtc gggcagttcg accttgacga tctcggcgcc ctcgcgcgtc agcacggcga
     1021 tggtgtcgtc gagcgccttc gctacctccg ggtcgagatc gtcgacatag aaggcggtgg
     1081 gcacgccgat cttgaggcct ttgaccgatc ccgatgtcgc cgccatgtag ttcggcaccg
     1141 ctgccgtact tgcggttgga tcggcggggt cggcgccggc catcaggccg agcagcagcg
     1201 cgcaatcttc cgccgtgcgt gcaagcggac cgaccgtatc gagcgactgc gacaacggca
     1261 tcgcgccggc ccggctgatg cggccgtagg tggtcttgag gcccgtgacg ccgcagaaat
     1321 gcgcgggcat gcggatcgag ccgccggtgt cggaaccgag cgctgcgaac gtaagccgcg
     1381 ccgcgaccgc ggagcccgac cccgacgacg aaccgccggt gatatgatcg cgcgcaaagg
     1441 gattgcggac ggcaccatag tgaacgttgt ggccggtcgg gccataggcg aactccacca
     1501 tctgcaacga accgaggcgg atctgccctg catccttcag gcgctgcagc gccgtcgagg
     1561 tgacactcgg gacaaaatcg cggcggatcg ccgagccgca ggtgacaatc ttgccggcat
     1621 cgtaatacat gtccttgtgc gccagcggca cgccatgcag cgggccacgg gattggcctc
     1681 tcgcgagtgc cgcgtcggcc tcctcggccg ccttcagtgc agcctcggcc tcgatcgtca
     1741 tgaaagcgtt cagcttcggt tgccattcgg cgatgcggtg caggcaggat cgcgtcactt
     1801 catgcgacga caggcgtttt tccgcgatcg ccttggcaac cgccgtcaac gacatcaggg
     1861 caggttcggt gctcatcgcg aaaccttcct cccctgaacg agcagaaagc ttgcaggctc
     1921 cagatcaaac ggcacggtgc cggcaacggg cgcaaagcct tcgaaggcag ggccgatcga
     1981 accggcgatg cgggtcgcga catcggcatc gacgggaacg ccggcgacat cg
//
LOCUS       DATLJT010001673         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3623061,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001673 DATLJT010000000
VERSION     DATLJT010001673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623061"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VM910_38145"
     CDS             <1..679
                     /locus_tag="VM910_38145"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVI68357.1"
                     /translation="LFYIAIKIFRLNEMIRIYRHGGPEILLVAASCGLVVALPIETGM
                     LLAIVLSFVHSLYFVVRPYCVELARVPGSTGWWPPGRGEKREHEPGVLVFAAAAPLTF
                     TNAQYISDKIMSALATAPAPVKLLVIEASGMIDIDYTGSQIFQETIAELRARSIDVAI
                     ARLSDPRAQAQADRTGLIAALGPERAFRTVEEAVRRLRPDAKPDDLGKKDDAGGRAAE
                     DPASGAP"
     gene            complement(739..1041)
                     /locus_tag="VM910_38150"
                     /pseudo
     CDS             complement(739..1041)
                     /locus_tag="VM910_38150"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(1481..2077)
                     /locus_tag="VM910_38155"
     CDS             complement(1481..2077)
                     /locus_tag="VM910_38155"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HVI68358.1"
                     /translation="MKELAVTPPPSSDLVPVTKANRQGIVDPLTALLFSAAAAGEGLS
                     PEACRHTLPIFDGHQRYDLKLAFKRMDKVTAEKGYAGPVVVCSVSYEPIAGHRANIPL
                     VKYLSEGREMEIALAPIAGTRLLAPFRLSVVSTLANLVIEANRFETSMAPAPAGTPPN
                     VAHPSDISPTRRDGVDERCVEVRSGVVVCQKVPEPAPK"
BASE COUNT          369 a          651 c          651 g          421 t
ORIGIN      
        1 tctcttctac atcgccatca agatctttcg tctcaacgaa atgatccgca tctaccgcca
       61 cggcgggccc gagatcctgc tggtggcagc gagctgcggg ctggtcgtgg cgctcccgat
      121 cgaaaccggc atgctgctgg ccatcgtgct gtccttcgtg cacagcctgt acttcgtggt
      181 ccggccctat tgcgtggaac tggcgcgcgt gcccggaagc accgggtggt ggccgcccgg
      241 ccggggcgag aagcgcgagc atgagccggg cgtcctcgtc tttgcggcgg cggcgccgct
      301 aacgttcacg aacgcccagt acatcagcga caagatcatg agcgcgctcg ccacggcgcc
      361 cgcgccggtg aagctgctgg tgatcgaggc cagcggcatg atcgacatcg actataccgg
      421 ttcgcagatc tttcaggaga ccatcgcgga gctgcgcgcg cgctcgatcg acgtcgcgat
      481 cgcccggctg tcggacccgc gggcccaggc gcaggccgac cgcaccgggc tgatcgcagc
      541 gctcggaccc gagcgcgctt tcagaaccgt cgaagaagcc gtgcgccggc tgcgtccgga
      601 tgcaaagccg gatgatctcg gcaagaaaga tgacgcggga ggccgtgcag cggaagaccc
      661 tgcgtcgggc gcgccgtgag cctgtcgtcg ggagctatcc ctttcggcgg gtggatggtc
      721 ggcagatgac cggcgcggtc aattggtctt catatatctc tcgtggcagc cgttgcaggc
      781 cgtccgcaac tccccaatca gccttttgaa ctcatccagg cgcttggtgg tgctcgcgtc
      841 ctgggcgagc ttggaggcgg tcgcggcgcg ccgatagaaa tccgcaaaat tcgcccagag
      901 gtcgggcgag gcgtaggtgt cggtcgcagg atcgcgatcg gcgcccgctt tccactggtt
      961 ggttgcagcc ggaaacatgt gcggaaacga catcaacatg atcgagatgg tatcggcgtg
     1021 ctcctgcgcc tccgcggcgt cgaacttgcc gttcgaccgt ggccatgtcg tctagtatcg
     1081 ccctgttcaa gtaaccctga atttgtgcgc gctttttcga atccatagtt cgtctcgtgt
     1141 cgcctagttt tgccctgtgc taccgagttg ggtagcaaaa atggtagcaa gattgtctgc
     1201 cccatggcct ccagcatggg aatccgaatt caccttgtcg cgattcccgt tctgcgcaga
     1261 atcgttccca tgttggcaat tcgaatcacc ggcaatcaac agcgcgcggg tcgcgcgctg
     1321 gcattcgata gccctgcaca gtggttctac cgcgccagcg gcctcgccaa tggcgggcgg
     1381 tcctattccc gccgccagct cctagcgaag gcggccatcg cagcctcgcg cgtcggctcg
     1441 aacttaggtg cgcggccgcg ccggaacgca gtatttagcg tcacttcgga gcgggctctg
     1501 ggaccttttg gcaaacgact acgccgctac gtacctcgac gcatcgttcg tcgacgccat
     1561 ctctgcgcgt cggtgaaatg tcggatggat gcgcgacatt agggggagtg cccgctggag
     1621 caggtgccat actcgtttcg aaacgattgg cctcgatcac taagtttgcg agcgtgctca
     1681 caaccgatag ccgaaacggc gccaataggc gcgtgccggc gattggtgcg agcgcgatct
     1741 ccatttcgcg gccctcggaa agatatttga caagcggaat attcgcgcga tggccagcga
     1801 ttggttcgta gcttacggaa cacacaacta cgggtcccgc gtaccccttt tccgctgtca
     1861 ccttgtccat tcgcttgaat gcgagcttca aatcatagcg ttggtggcca tcgaagatcg
     1921 gtagtgtgtg ccggcacgcc tctggggaca aaccttcgcc tgcggcagcg gccgaaaaca
     1981 gcaatgcggt caacggatcc acgatacctt ggcgattagc cttggtcact ggcacgagat
     2041 ccgaactcgg cggcggtgtg accgccagct ccttcacatt cccttcctcg ag
//
LOCUS       DATLJT010001674         3432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_174185,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001674 DATLJT010000000
VERSION     DATLJT010001674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174185"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VM910_38160"
     CDS             <1..1255
                     /locus_tag="VM910_38160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68359.1"
                     /translation="TSGREQDVATAKTDALTRWREQRNLPWLVAALMHLSPGDEDVAA
                     AIAASRAIEASSPAYYTLAWNRLRLLIGENKQEEARTELDQILDGRPLPEGVENLMRY
                     HRMKLARDLDEFARFALRRGEFVMYLPDPRTKLDATAPPLKSSTSFPYDFGAMLRWRT
                     ELFQPNPRYFDTDATAAMSFVMPLPLMARVAQSDRLPPNMQRDVALAVWTRAVLLDDA
                     EIANSVTPTVARHFPQSGAGWKAYQSAATPQQKKIEAALLLLRLPGASPWFAAGLGYP
                     YMRDVIGLVATRWWERGDADNQAVPDAPDDVTLCDDCALPLQFCPAGLPHVAGSRSRA
                     QRGRTLAPASRSARLSRRHHYRLGQCPAARSAGARGASPGGAGNAIRANEYRDVEGSL
                     SAAAQSLSPQSLDVENTPLVYSAPR"
     gene            complement(1452..1676)
                     /locus_tag="VM910_38165"
     CDS             complement(1452..1676)
                     /locus_tag="VM910_38165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68360.1"
                     /translation="MTTSDDVQKRNAFAACAAFRIEGLEASLDHAREIAVRDELGAVG
                     HYIAEAQGYLAQIRLLHQEALDEFSRAQGE"
     gene            2164..2403
                     /locus_tag="VM910_38170"
     CDS             2164..2403
                     /locus_tag="VM910_38170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68361.1"
                     /translation="MKALAISIVAGAALCSAVAVPASAIPMSNLPAAASDLTPGESVR
                     YVRNHYRDQDSSNGNAGDTANCYGYGFGPSSPCYP"
     gene            2545..>3432
                     /locus_tag="VM910_38175"
     CDS             2545..>3432
                     /locus_tag="VM910_38175"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HVI68362.1"
                     /translation="MDARTPLQPAVVTPKQALSIKPFGLDMDVLLTTEATGGATSVIL
                     GWHKPGEGPPDHVHFKQEEILFIIEGLYEVTVGDATAKAGPGSIVFIPRNVVHRFKNV
                     GGITGRMLGWSLPGGQDHYLKAISELAAGGGFTSEKVMEISKTFDTHFPVEPNARNSD
                     PFVHTDGHSLERIMQASTPEPILKISTGFMAAKQLFAASEIGLFEALADGPAGLEELA
                     GKTAVPPRTVGIVAAAMVSLGLIDQEGGRYRNGEAAATFLAGKPGHDVRPVLRFFNSI
                     SYPLWERLADAVRSGEGQAQ"
BASE COUNT          633 a         1103 c         1047 g          649 t
ORIGIN      
        1 cacctccggt cgcgagcagg acgtagccac cgccaaaacg gatgcgctca cccgatggcg
       61 cgagcagcgc aatctgccgt ggctcgtcgc cgcgctgatg catctctccc ccggcgacga
      121 ggatgtcgcg gctgcgatcg ccgcctcgcg cgcgatcgag gcgagctcgc ccgcctatta
      181 cacgttggcc tggaatcgcc tgcgcctgct catcggcgag aacaagcagg aggaagcgcg
      241 caccgagctc gatcaaatcc tcgacggccg tccgctgccg gaaggggttg agaacctgat
      301 gcgctatcac cgcatgaagc tggcgcgcga tctcgacgag tttgcccgct tcgcgctgcg
      361 ccgcggcgaa ttcgtgatgt accttcccga cccgcgcacc aagctcgacg cgacggcgcc
      421 gcctttgaag tcgtcgacga gctttccgta cgacttcggc gcaatgctca ggtggcggac
      481 ggagttgttt cagccgaacc cacgatattt cgacaccgat gcgacagcgg ccatgtcgtt
      541 cgtcatgccg ctgccgttga tggcccgggt ggcgcaatct gaccggctac cgcccaacat
      601 gcagcgcgac gtggcgctgg cggtgtggac gcgcgcggtg ctgctcgacg acgccgagat
      661 tgccaattcc gttacgccca ccgtcgcgcg tcactttccc caatccggcg cgggctggaa
      721 agcctatcag agcgccgcca cgccgcagca gaagaagata gaagcggcgc tgctcctgct
      781 acggctgccc ggggcaagtc cctggttcgc ggcggggctc ggctacccct acatgcgcga
      841 cgtgatcggg ctcgttgcaa cgcgctggtg ggagcgtggg gacgccgata atcaagccgt
      901 gcccgacgcc ccggacgatg tcacgctttg tgacgattgc gcccttccgc tgcaattttg
      961 ccccgccggc cttcctcacg tcgcaggatc gcgatcgcgc gcgcaacgag gtcgcacgct
     1021 tgcgccagct tcccggagcg cccgcctatc tcggcgccat cattatcgcc tgggccaatg
     1081 cccagccgcg cgatccgcgg gtgcccgagg cgcttcacct ggtggtgcgg gcaacgcgat
     1141 acgggcaaat gaataccgag acgtcgaagg cagcctatct gctgctgcac aatcgctatc
     1201 gccgcaatcc ctggacgtcg aaaacacccc tttggtttac agcgccccgc gatgaatgag
     1261 ggtgagaggg cccctgtcat tcaaagcgcg gcgctgagtt gttgtcatat cgaagagcgc
     1321 gcgccttgcg gcggacggcg cggcttgcag agcgagcttt aggcccaacg atgtgcggca
     1381 ggcgcttgct gccgtcgttc gagcgctgac cgcgcgaggg tttcaaaggc cgcacggcgg
     1441 agcgagagcc tttattcgcc ctgcgcgcgg ctgaactcgt ccagcgcctc ttgatgcagg
     1501 aggcgaattt gagccaggta tccttgcgcc tccgcgatgt agtgccctac ggcaccgagc
     1561 tcgtcgcgga ctgcgatctc gcgcgcgtga tccagggagg cttcaaggcc ctcgatgcga
     1621 aacgcagcac acgcggcaaa ggcatttcgc ttttggacgt cgtcgctggt cgtcatggct
     1681 ccatcctcac gtttgccgga acctagcgtc gcgcgggggc gtcgatcttc tgttttgccc
     1741 cctccatgtc gccacggcct cggcggattg ctgacgtgac ctgtcttcat gaccttcttg
     1801 ggctggtggc atagttcacc agagttggct taaggtcctt gggctagaac tgaattgttc
     1861 tgaatcgccc tgaatccctt cgctaccttg cctttcaaat cgcttattga acctctcctt
     1921 gccgccgcgc agttccatca ggggatgttc gtcgaaataa cggcatgccc gcccccgcgc
     1981 atgtcatccc cggcttttgc acgattggcg caacttcggc gaagtcagag caagaacgcc
     2041 aaagttactt tttccaatgg ccatacgtgt acgaccgcac caggcttcgg tcgcaggccg
     2101 gcatccgaca aggaaacggt cgcgattcat cgattgaagg tgcagcaaca ctagaggtgt
     2161 tccatgaaag ctctcgcgat cagtatcgtc gccggcgcag cgctatgcag cgccgttgct
     2221 gtgccggcat cagcgatccc catgagcaat ctccccgccg cagccagtga cctcacgccg
     2281 ggcgaaagcg tgcggtatgt ccgcaaccat taccgggacc aagacagctc taacggcaat
     2341 gccggcgata cggcgaactg ctacggctac ggctttggac caagctcccc ctgctatccg
     2401 taaaggtagc gcgtagctcg gaggatgggt tgagcgcagc gaaacccctc ctccacttta
     2461 ttcgaggcgt tttgccgaca tctcgcgtcc ggcgatgcga gcctcgtcga actagaaggt
     2521 cgtgaagaac tggagacagc atccatggac gcgcgaaccc cattgcagcc cgctgtcgtt
     2581 acgcccaaac aggcgttgtc gatcaagccc ttcggcctcg acatggacgt gctcctcacc
     2641 actgaggcca ccggcggcgc cacttctgtg atcctggggt ggcacaagcc cggtgaagga
     2701 ccgcccgacc atgtccattt caaacaggaa gagattttgt tcatcatcga gggcctctat
     2761 gaggtgaccg tcggcgatgc gaccgccaag gcgggtcccg gcagcatcgt cttcatcccg
     2821 cgcaacgtcg tgcaccgctt caagaacgtc ggcggcatca cgggacgcat gctgggctgg
     2881 agcctgccgg gcgggcagga tcattacctc aaagcgatct ctgagcttgc cgccggcggc
     2941 ggtttcacca gcgagaaggt gatggagatc agcaagacgt tcgataccca cttccctgtc
     3001 gagccaaatg cccggaattc tgatccgttc gttcacacag acggccacag tttggagcgc
     3061 atcatgcagg cgagcacacc cgaaccgatc ctgaagattt ccaccgggtt tatggcggca
     3121 aaacaacttt ttgcagcaag cgagatcggt ttgtttgagg cgcttgccga cggaccggct
     3181 ggcctggaag agctcgcggg caagaccgcg gttccgccgc gcacggtagg aattgtagcg
     3241 gctgcgatgg tgagcctcgg attgatcgac caggaaggcg gccgctatcg taatggagag
     3301 gcggcagcga catttcttgc cggcaagcca ggtcacgatg tgcgacccgt cctgcgcttc
     3361 ttcaacagca tcagctatcc gctctgggaa aggctcgccg acgccgtacg cagcggtgag
     3421 ggtcaggcac ag
//
LOCUS       DATLJT010001675         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_636935,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001675 DATLJT010000000
VERSION     DATLJT010001675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636935"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VM910_38180"
     CDS             complement(<1..933)
                     /locus_tag="VM910_38180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68363.1"
                     /translation="MKLPRRNFLHLAAGAAALPTLSRIARAQAYPSQPVRIIVGLPAG
                     TATDILARLVSQSLSERLGKQFIVENRPGAAGNIATEVVMRASPDGYTLLQVNSGNAV
                     NASLYANLNFNFIRDITPVACIARTPFVMVVNPSSPAKTVPEFIAYAKANPGKINMAS
                     PGIGSANHIFGASFAMMAGIDLVHVPYRGDFMADLLGGRVQVVFSAMAQSIEYIRDGK
                     LRGLAVTTAARLDVLPDIPAMDEFVTGYEASGWQGIGAPKNTPTNIVDRLNNEINAIV
                     ADPQVKVRLLDMGFKPTFMTSAEFGKFIADETEKW"
     gene            complement(952..1911)
                     /locus_tag="VM910_38185"
     CDS             complement(952..1911)
                     /locus_tag="VM910_38185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68364.1"
                     /translation="MLLGSTAAAWPLAVRAQQPAMPVVGFVSGRSPSSDAHLVAAFRR
                     GLNESGYVEGQNVAIAFRWAEGQNDQLASLVADLVRSRVTVIAATSIAAALAAKVATT
                     TIPIVFRIGGDPVGLGLVTSLNRPSGNLTGVTSLNIEVGSKRLELLHELVPTAATVGI
                     LVNPTSPNADKTSRDLEAAAQTLGLHLRILHAHTERDLDAALASLVQVDAGGLVIGPD
                     ALFSSQNEKLAALTARYAVPSVHYLRSFVTAGGLMSYGGSFTDSFHQTGAYVGRILKG
                     ERPTDLPVQQATKVELTINLKTAKALGLTVPDTLLARADGVIE"
     gene            complement(2010..2420)
                     /locus_tag="VM910_38190"
     CDS             complement(2010..2420)
                     /locus_tag="VM910_38190"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HVI68365.1"
                     /translation="MPFYTCFAFSDSLSAAQKAKIAEEITRIHTKLTGAPRSFVRVLF
                     HEMQSSDIFTGGKPLPFAMIRGVIRAGRSAEVKKQLLSELWTMLQDATGLKDDKLLVS
                     IQDNPANNAMEAGEILPEPQHEAEWFAGLGRTGH"
BASE COUNT          509 a          763 c          782 g          554 t
ORIGIN      
        1 ccatttctcg gtttcgtcgg cgatgaattt cccgaattca gcggaggtca taaatgtcgg
       61 cttgaagccc atgtcgagga ggcgcacctt cacctgagga tcggcaacga ttgcgttgat
      121 ctcgttgttg agccggtcaa caatgttggt gggcgtattt ttcggggcgc caataccttg
      181 ccaaccgctc gcctcgtagc cggtcacaaa ttcgtccatg gctgggatgt cgggcagcac
      241 atccaagcgg gccgcggtgg tcactgcgag gccgcgcagc ttgccgtctc tgatgtactc
      301 gatagactga gccattgcac tgaacacgac ctgcacccgc ccaccgagca gatcggccat
      361 gaaatcgccg cgatacggca cgtgaaccaa gtcgatgccg gccatcatcg cgaacgatgc
      421 gccgaagata tggttcgcgc tgccgatgcc gggcgaggcc atattgatct ttcccggatt
      481 agccttggca taggcgatga actcggggac cgttttggcc ggcgatgatg gattaaccac
      541 catgacgaag ggcgtgcggg cgatgcacgc gaccggcgtg atgtcgcgga tgaaattgaa
      601 atttaggttc gcgtagagcg acgcattcac cgcattcccc gaattgactt ggagcagcgt
      661 gtagccgtcc ggggacgccc tcatgacgac ttcggtggcg atattgccgg cggcgcccgg
      721 tcggttttcg acaatgaatt gtttgccgag ccgctccgat agcgattggc tcacgaggcg
      781 cgcgaggatg tcagtcgcag tcccggcggg caagccgaca ataatgcgca ccggctgcga
      841 tggataggct tgcgcccgcg cgatgcgaga tagcgttggc agtgcggcag cgcctgcggc
      901 cagatgcaga aaatttcggc ggggaagttt cattttgaac ctcccttcac atcattcgat
      961 cactccgtcg gcacgcgcga gcagcgtatc cggcacggtt aggccgagcg ccttggcggt
     1021 cttgaggttg atggtcagct cgacttttgt cgcctgctgc actggaaggt cggtcggcct
     1081 ctcgcctttg agaatccggc cgacataggc gccggtttga tgaaaggaat cggtgaaact
     1141 gccgccgtag ctcatcaggc cgcctgcggt gacaaagctg cgtaaatagt gcaccgacgg
     1201 aaccgcatag cgggcggtca gcgcagcgag cttttcattc tggctcgaga ataatgcatc
     1261 agggcctatg acgagccctc ctgcatcgac ttggaccaga cttgccaatg cggcatcgag
     1321 atcgcgttcg gtgtgggcat gcaggatgcg caggtgcagc ccgagcgtct gggccgccgc
     1381 ctccaggtct ctcgatgtct tgtcggcgtt aggactggtt gggttgacca ggatgcccac
     1441 tgtggctgcg gtgggcacca actcgtgcag cagctccagc cgcttcgacc ccacctcgat
     1501 gttcaacgag gtcacgcccg tcaggttgcc gctcgggcga ttgaggctgg tcaccaatcc
     1561 cagccccaca gggtcgcccc ctatcctgaa gacaattgga attgtcgtgg tcgctacctt
     1621 cgcagccaag gccgcggcaa tgcttgtcgc ggcaatgacg gtcactcggc tgcgaactag
     1681 atcggccaca agggaggcca attgatcgtt ctgaccttcc gcccagcgaa acgcgattgc
     1741 gacgttctgg ccttcgacat agccggattc attcaggccg cgtcgaaacg cggccacaag
     1801 atgcgcgtcg gagctgggcg acctgccgct cacgaatccg accaccggca tcgccggctg
     1861 ctgcgcccgc accgcgagcg gccaagccgc agccgtgctg ccgaggagca tgatgaacct
     1921 gcggcgctta taaagaaaac aagaagccat gggacgatta ccaagcgtgt cggtttggcc
     1981 aaaaggccga gatcggccaa gggaagcgat caatggccgg tacgccccag ccccgcgaac
     2041 cactcggctt cgtgttgggg ctcggggaga atttcgccag cttccattgc attgttcgcg
     2101 ggattgtctt ggatgctaac aagcaacttg tcgtctttca gccccgtcgc atcctgcagc
     2161 attgtccaaa gctcagatag gagttgcttt ttcacttccg ctgatcgccc ggcccggatc
     2221 acaccacgga tcatcgcgaa tggcaggggc ttcccgcccg tgaagatatc ggaggattgc
     2281 atctcgtgaa acaaaaccct gacgaagctc ctcggagcac cggtgagctt cgtgtgaatc
     2341 cgagtaatct cttcggctat ctttgctttc tgcgcagccg aaagagaatc cgagaatgca
     2401 aagcaggtat agaatggcat tatgccctcc tgttcaatgt tgatctcgtg ccagtcaccc
     2461 ttgtcctgtg gtgttttcgt aacgttccgt ggctaccggt cacccttttg atatgttccg
     2521 ataagctctg cattggccag gacgtggccc cgcattgctc gctcgatctg cgatttcgtc
     2581 gcgtctctta gatcgagcgt gatatcca
//
LOCUS       DATLJT010001676         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2474203,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001676 DATLJT010000000
VERSION     DATLJT010001676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474203"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VM910_38195"
     CDS             complement(<1..504)
                     /locus_tag="VM910_38195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI68366.1"
                     /translation="MPDATVTAAPTSRAERAAERRSAIVAAALDEFVARGFTATRLDD
                     VAKRAGVAKGTIYLHFKDKEALFEELVRTAIVPVVERITTPPALTGSRSIRDALEGFA
                     RTFIEEVANTRRADIIRLIVAEGPRFPAIADFYYREVISRGLAAMRALIEAGIARGEI
                     RSSELKDF"
     gene            complement(790..1200)
                     /locus_tag="VM910_38200"
     CDS             complement(790..1200)
                     /locus_tag="VM910_38200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HVI68367.1"
                     /translation="MQFDTKIAVVVRTDLEPWQKLNVASFLSGGIAAAFPGCVGEAYE
                     DGSGTKYLSLIGQPILIYGADRPALSRALERALARNVTPAVYTEDMFRTTHDQANRAA
                     VKGVPRAELNLVGLAFRAERKLVDKIVDGLKFHT"
     gene            complement(1256..1645)
                     /locus_tag="VM910_38205"
     CDS             complement(1256..1645)
                     /locus_tag="VM910_38205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HVI68368.1"
                     /translation="MVSNRLAQRRALFVFALILAIAGARFVTQSWAAGAFAMGKCGAY
                     GQAFDLAGEDEARVAALKQCKGECTAVTMKRSCAAFAVDMKDPCGPHGYAVRPHISSS
                     LNAATRECYKFGGKECVIRAWACDAKG"
     gene            complement(1917..3521)
                     /locus_tag="VM910_38210"
     CDS             complement(1917..3521)
                     /locus_tag="VM910_38210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HVI68369.1"
                     /translation="MKFGPASPADAIGGVTVHTLRQGSLVLKKGTTIGPAEAAALGQA
                     GVSEIVVVRLEPGDVSEDEGAASIAQAVTGDGINVERAFTGRANLFAARAGVLVVDRA
                     AVDRINGVDEAITLATLPAFKPVVEGEMIATVKLIPFGVEGRLRDAAVKAAGKGALSV
                     APYVIKRIGIVSTQLPGLSPKVIDKTLRVTAERLAPAGATIMAERRVPHEERALATAI
                     KELLALGAELVIVFGASAIADRRDVIPAAISDVGGAIEHFGMPVDPGNLLLIGSAGGV
                     PVLGAPGCARSPVENGFDWVLMRLLAGLKVTRAELMGMGVGGLLMEIVTRPQPRAKPE
                     AADNRNVAAIVLAAGRSTRMGGPNKLLAEISGKKLVRIAAEQALASKASEVIVVTGHQ
                     AELVEQALEGSNVKFARNPDFAGGLASSVKAGIAAVSDNADGAVICLGDMPLIDAKLI
                     DRLIEAFAPDRGNLIVVPVAEGRRGNPVLWSRRFFNELMTLDGDIGARHLIGKHAEAV
                     AEVPVEGNGAFLDIDTPQALETARQG"
     gene            complement(3518..>3934)
                     /locus_tag="VM910_38215"
     CDS             complement(3518..>3934)
                     /locus_tag="VM910_38215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVI68370.1"
                     /translation="LDYDVTVVDPRTAFASPERFPDVPLIAEWPDVALPPLNIDHYTA
                     FVALTHDPKIDDPALLHAFQRECFYIGALGSRKTHAKRAERLRAQGAKESDIARIHAP
                     IGLPIGAVSPSEIAVAIMAEITAQLRLPPKEKEQAA"
BASE COUNT          691 a         1310 c         1224 g          709 t
ORIGIN      
        1 aaaatctttc agctccgatg agcggatttc gccgcgcgcg atcccggcct cgatcagcgc
       61 ccgcatggcc gcaagcccgc gcgagatcac ctcgcggtag tagaaatcgg caatcgcggg
      121 gaagcgcggc ccttcggcca cgatcagacg gatgatgtcg gcccgacgcg tgttggcgac
      181 ctcctcgatg aaggtcctgg caaacccttc gagcgcgtcg cggatcgagc gcgagcccgt
      241 cagggccggc ggcgtggtga tgcgttccac gaccggcacg atcgcggtgc gcaccagctc
      301 ctcgaacagc gcctccttgt ccttgaagtg cagatagatc gtgcccttgg cgacgccggc
      361 gcgcttggcg acgtcgtcga gcctcgtcgc ggtgaagccg cgggcaacga actcatccag
      421 cgccgccgcg acgatggctg atcgccgctc ggcagcgcgc tcggcgcggc ttgtgggcgc
      481 ggctgtcact gtcgcgtcgg gcatggttcc tccgccgtcg ggatgcggtt acctctttca
      541 attatcaata atgactgact ggtcagtcat agtcaatgac caaaaaggca actcttcgtg
      601 aatgggctgg ttccctgcga tttcaggcat ccctcagggc ggcgcccaat tccggcacgc
      661 tatgcgcctg gatttaaacg ccgcccctcc gcgtcccccg ccgagcccca ttgaggcccg
      721 actaacgatc acaaacgcgg ctgctcgcgg ccatgatccg gatcaattcg gaggtggttt
      781 tcggtgggat caggtatgaa acttcaggcc atcgacgatc ttgtcgacca atttgcgctc
      841 ggcgcgaaag gcaagcccga ccaggttgag ttcggcgcgg gggaccccct tcacggccgc
      901 gcggttggct tggtcatgcg tggtcctgaa catgtcttcg gtatagacgg cgggcgtcac
      961 attacgggcg agcgcccgct ccagggcacg cgacagcgct gggcggtccg cgccgtagat
     1021 caggatcggc tggccgatca gcgacaggta tttcgtgccc gagccgtcct cgtaagcttc
     1081 gcccacgcat ccgggaaagg cggcagcaat gccgccggac aggaatgacg ccacgttgag
     1141 cttctgccac ggctcgaggt cggtgcgcac gaccaccgcg atcttggtat cgaattgcat
     1201 gcttgctccg ccgtcatgag agcgcgatgg caattcgaca tgaagctatc gcgccctagc
     1261 ccttcgcgtc gcaggcccac gcgcggatca cgcattcctt gccgccgaat ttgtagcatt
     1321 cgcgcgtcgc ggcgttaaga gatgacgaaa tatgcggacg caccgcataa ccgtgcggcc
     1381 cgcaggggtc tttcatgtcg acggcaaatg cggcgcaaga gcgcttcatg gtgaccgcgg
     1441 tacattcgcc cttgcactgc ttcagcgctg cgacgcgcgc ttcatcctcg ccggcaaggt
     1501 caaaggcctg gccgtaggca ccgcattttc ccatcgcgaa cgcacctgcg gcccatgact
     1561 gcgtgacaaa ccgcgcgccc gcgatcgcca agatcaaagc aaagacaaaa agtgcgcggc
     1621 gctgcgccaa tctgttcgaa acgatcaaaa gcccctcccc actccaacgg cgggaaacta
     1681 tccgagcggg tctttccact tggtgaatgc gaggtcgaaa ataaaccaaa cactcggcac
     1741 tccacgatcg cctgacgatc gcgccctacg tcccgctgca ggcagaacga gaccagactc
     1801 gttatcaaaa atttgatttg tggttggttg agcgaagcga aatcaatttt ctttttggag
     1861 cgattcgcac ggtgggttcg ctttgcttta cccacggtgc gaatcacgaa ttcgttttag
     1921 ccttgccgcg cggtctccag tgcctgcggc gtatcgatgt cgagaaacgc gccgttcccc
     1981 tcgaccggta cttcagcgac cgcttcggca tgcttgccga tcaaatgtcg cgcaccgata
     2041 tcgccatcga gcgtcatcag ctcgttgaag aaacgccgcg accacaagac cggattgccg
     2101 cgccgtcctt cggccacggg cactacgatc aggttgccgc gatcgggtgc aaacgcctcg
     2161 atcagccgat cgatcagttt tgcatcgatc agcggcatgt cgccaaggca gatcacggcg
     2221 ccgtcggcat tgtcggacac cgctgcgatc cccgctttca ccgagctggc gaggccgccg
     2281 gcgaaatccg gattgcgtgc gaatttgacg ttcgaccctt cgagcgcctg ctcgaccagt
     2341 tcggcctgat gcccggtaac gacaatcacc tcggacgcct ttgaggcgag cgcctgctcg
     2401 gccgcgatcc gcaccagctt cttgccggaa atttcagcga gcagcttgtt cgggccgccc
     2461 atccgggtcg agcgtccggc ggcaagcacg atcgcggcga cgttgcggtt gtctgcggct
     2521 tccggcttgg cgcgcggctg cggccgggtg acgatttcca tcaacaggcc gccgacgccc
     2581 atgcccatca gttcggcgcg ggtgaccttc agccccgcga gcaggcgcat caacacccag
     2641 tcaaagccgt tctcgaccgg cgaacgcgca cagccgggcg cgcccaggac cggcacgccg
     2701 ccggcgctgc cgatcaggag cagattgccg ggatcgaccg gcatgccgaa atgttcgatg
     2761 gcgccgccga cgtccgatat cgcagcgggg atcacgtcgc gccgatcggc gatcgccgac
     2821 gccccgaaca cgatcacgag ctcggcgccg agcgcgagca actccttgat tgcagtggcg
     2881 agcgcccgct cttcatgcgg cacccggcgc tccgccatga tcgtggcgcc ggcgggcgcg
     2941 agcctttcag cggtcacgcg caacgtcttg tcgatcactt tgggcgaaag gcccggcagt
     3001 tgggtcgaga cgatgccgat gcgtttgatg acgtagggcg caacgctcag cgcgcctttg
     3061 cctgccgcct tcacggcggc gtcgcgcagc cgtccctcga cgccgaacgg gatcaatttg
     3121 acggtcgcga tcatctcgcc ttcgaccacc ggcttgaaag ccggcagcgt cgcaagcgtg
     3181 atcgcctcgt cgacgccgtt gatgcgatcg acggcggcgc gatccaccac cagcacgccc
     3241 gcgcgcgcgg caaacaggtt ggcgcgcccg gtgaaggcgc gctcgacatt gataccatcg
     3301 cccgtgacgg cctgcgcgat cgaggccgcc ccttcgtcct cggagacgtc gcccggctcc
     3361 agccgcacca cgacgatctc gctcacaccc gcctgcccga gcgccgcggc ctcggcgggc
     3421 ccgatcgtcg tgcctttctt cagcaccaat gatccctggc gcagcgtgtg gacggtcacc
     3481 ccgccaatcg catctgccgg gctggcagga ccgaacttca tgcggcttgt tctttttctt
     3541 tcggcggcag gcgaagctgc gcggtgattt cggccatgat cgcgaccgcg atctcggacg
     3601 gcgagaccgc gccgatcggc aggccaatcg gcgcatgaat gcgcgcgatg tcgctttcct
     3661 tcgcgccctg cgcccgcaac cgctcggcgc gctttgcgtg ggttttccgc gatccgagcg
     3721 cgccgatgta gaagcactcg cgctggaagg cgtgcagcag cgccgggtcg tcgatcttgg
     3781 gatcgtgcgt cagcgcgacg aaggcggtgt agtgatcgat gttgagcggc ggcagtgcga
     3841 cgtcgggcca ttcggcaatc agcggcacat cgggaaagcg ctcggggctt gcaaaggccg
     3901 tgcgcggatc gacgaccgtc acatcatagt ccag
//
LOCUS       DATLJT010001677         3426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1554739,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001677 DATLJT010000000
VERSION     DATLJT010001677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554739"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VM910_38220"
     CDS             complement(<1..1827)
                     /locus_tag="VM910_38220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68371.1"
                     /translation="MSDGRKTKFKEPMQDVKLPKLEVESEAKPETPLTAEEIYLKRKA
                     EVAQQDNEDFSAYLREKAARDEQQAPQPAPAQTAPLPQTAPRKLNRRSLLTWAAGTAV
                     VGEAVTLGYPMLTGQNTPDHGARHAGSTNTESQRHPIDRELVNPQADVGGRRFGDWVL
                     LMPTKMGGGTYAVNLNTGRALAWVSYWNYGDYNPISHHLCAFPSADPAKGFEWINSTQ
                     GGKNSLICGIPTQIETPGEGFNIYRVRYDGSQMEVMENVAETTGLGLGVHVNIDPKTA
                     ERYFVTDGQKDIAACFDRRTSRVIAALKYDWVPNVRNLAEAWQKGGVLKISKIYPDPA
                     TGKYDYLGTKGQKIEWEMVPMGELFVEEGTLPGDDVLSLCGADGTIWHPSGRWASTIV
                     RLCGGQLILDAEKNFEPVTFLQFNKNAPNQYPVKKIDNDHWEVTFDKIFSPGHEIGFS
                     PDGRFLCMMNNLRENNCSVFDSSDPDPRNWKKIAHIEDPLWVGKYPNPFHMVFSMDGS
                     KLYLSILHPSPAASGVMVVDTKTWKIKKEIQGIGPDMQTLAVTYDGKYVVGVFSGFQR
                     LSSGIAFIDTETDELVGIWPSNGGHHDCVIVPRTLEHMKHTRS"
     gene            complement(1929..3347)
                     /locus_tag="VM910_38225"
     CDS             complement(1929..3347)
                     /locus_tag="VM910_38225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68372.1"
                     /translation="MHSSRRKVGDVTLSAQVLTKRYGDAPGYAAVRGVSLELSRGEFV
                     SIVGRSGSGKSTLMAMLGALTMPTEGKLLLDGTDVWTLPESERATFRSRHVGFVFQFP
                     SLLSNLTAVDNVAVPALLGRTMEAEEAYSRAHDLIARVGLTDRADAYPGSMSGGEQRR
                     VAVARALINAPPLLLADEPTSDLDEDSETDIIDLLERLQRTESFGFVLVTHDPKLAKR
                     AQRSYEMRQGALAATDLPQAVAEPQRRVTPAKIRPAQPAPAAARAPIRLGANLWGGVK
                     TFLIAAAVIFAAVLAADVAVEKYQAMRVRERAARVAALQHLALNGLRGDVQSVADLGD
                     GRYELTTYLENVGGAEPIYVMAPDMRAYVQVGTVWQEVPMTAADDSATGVTKIERKQT
                     YRYRFDARVRDFAQLLPNYMHVRFSGTMLVSPSSTPNGDVFERKDNYYVYLKPFDVAD
                     EVVLRRMRFSGKPPVWIPMPPH"
BASE COUNT          557 a         1077 c         1139 g          653 t
ORIGIN      
        1 tgagcgcgtg tgtttcatgt gctcgagcgt cctcggcacg atgacgcagt cgtggtggcc
       61 gccgttgctc ggccagatgc cgaccagctc gtcggtctcg gtgtcgatga aggcgatgcc
      121 gctcgagagc cgctggaagc cgctgaacac gccgaccacg tatttgccgt cgtaggttac
      181 cgccagcgtt tgcatgtccg ggccgatccc ctgaatctcc ttcttgatct tccaggtctt
      241 ggtgtcgacg accatcacgc cgctggcggc cggggagggg tgcaggatcg aaaggtagag
      301 cttggacccg tccatggaga acaccatgtg gaacggattc ggatatttgc cgacccacag
      361 cggatcctcg atgtgcgcga tcttcttcca gttgcgcggg tcgggatcgc ttgagtcgaa
      421 gaccgagcaa ttgttctcgc gcagattgtt catcatgcaa aggaagcggc cgtcggggga
      481 gaagccgatc tcgtggccgg gcgagaagat tttgtcgaac gtcacctccc agtggtcgtt
      541 gtcgatcttt ttcacgggat attggttggg cgcgttcttg ttgaactgaa ggaaggtcac
      601 gggctcgaag ttcttctcgg catcgaggat gagctggccg ccgcacaatc gcacgatggt
      661 ggacgcccag cggccgctcg ggtgccagat ggtgccgtcg gcgccgcaga gactaagaac
      721 atcgtcgccg ggaagcgttc cctcctcgac gaacagttcg cccatgggca ccatctccca
      781 ttcgatcttc tgccccttgg tgccgagata gtcgtacttg ccggtcgccg ggtcgggata
      841 gatcttggag atcttgagca cgccgccctt ctgccaggcc tcggcgagat tgcggacgtt
      901 gggcacccag tcgtatttga gcgccgcgat cacgcgcgag gtccggcggt cgaagcacgc
      961 cgcgatgtcc ttctgcccgt cggtgacaaa atagcgctcc gcggtcttgg gatcgatgtt
     1021 cacatgcacg ccgagaccaa gccccgtcgt ttccgcgacg ttctccatca cctccatttg
     1081 cgagccgtcg tagcgcacgc gatagatgtt gaagccttcc cccggcgtct cgatctgggt
     1141 gggaatgccg cagatcagcg aattcttgcc gccctgggtg ctgttgatcc attcgaagcc
     1201 cttggccgga tccgcgctcg ggaaggcgca caagtgatgc gagatcgggt tgtagtcgcc
     1261 gtaattccaa tacgagaccc aggcgagcgc cctgccggta ttcagattca cggcataggt
     1321 gccgccgccc atcttggtcg gcatcaaaag aacccagtcg ccgaagcgtc gcccacccac
     1381 atccgcctgc ggattgacca attcgcgatc gatgggatgg cgctgggatt cggtattggt
     1441 tgagccggcg tgcctcgcgc cgtgatccgg ggtgttctgc ccggtgagca tgggatagcc
     1501 gagcgtgacc gcctcgccca ccaccgcggt gcccgcggcc caagtcagga ggctgcgccg
     1561 attgagtttc cttggtgcgg tttgcgggag aggcgcggtt tgcgcaggcg ccggttgcgg
     1621 cgcttgctgt tcatcgcggg cggctttttc ccgcagatac gccgaaaaat cctcgttgtc
     1681 ctgctgcgcc acctccgcct tgcgcttgag gtagatctct tcggcggtga gcggcgtctc
     1741 gggcttcgcc tcgctctcga cctcaagctt cggaagcttg acgtcttgca tcggctcttt
     1801 gaatttcgtc ttgcgcccgt cggacatggc ccagctcctc cgcttcccga gttacgactg
     1861 acgttgattg cacaataatg tcgcgcagcg atcaagatcg actgccgcgc ggtcgatgca
     1921 tcggcacctc agtgcggggg catggggatc cagaccggtg gttttcctga aaaccgcatg
     1981 cgcctcaaga caacttcgtc ggccacgtcg aacggcttga gatagacgta gtaattgtcc
     2041 ttgcgctcga acacgtcgcc gttcggcgtg ctgctcggac tgaccagcat cgtgccggag
     2101 aagcgcacgt gcatgtagtt ggggagcagt tgcgcgaagt cgcgcacgcg cgcatcgaag
     2161 cgatagcgat aggtctgctt gcgctcgatt ttcgtgacgc cggttgcgct gtcatcggcc
     2221 gccgtcatcg gcacttcctg ccacaccgtt ccgacctgaa cataggcgcg catgtccggc
     2281 gccatgacat agatcggctc cgcgccgccg acgttctcaa gatacgtcgt caattcgtag
     2341 cgcccgtcgc cgaggtcggc gaccgactgc acgtcgccgc gaaggccatt gagcgccagg
     2401 tgctgcagcg ctgcgacgcg ggcggcgcgc tcgcgcaccc gcatcgcctg atatttctcg
     2461 acggcaacgt cggccgcgag gacggcggcg aagatcacgg ctgcggcgat gaggaaggtc
     2521 ttgaccccgc cccacaggtt cgcgccgagg cggatgggcg cgcgcgccgc ggccggcgcc
     2581 ggctgcgcgg gacggatctt ggccggggtg acgcgccgtt gcggctcggc cacagcctgg
     2641 ggcaggtcgg tcgccgcgag cgcgccttgg cgcatctcgt aactgcgctg cgcgcgcttg
     2701 gcgagcttag gatcgtgggt gacgaggacg aagccgaacg attccgtgcg ctgcaaccgt
     2761 tcgagcaggt cgatgatgtc ggtctcgctg tcctcatcga ggtcgctcgt cggttcgtcg
     2821 gcgagcaaca gcggcggcgc attgatgagc gcgcgggcga cggcgacccg gcgctgctcg
     2881 ccgccggaca tgctgccggg gtaggcgtcg gcgcggtccg tcagcccgac gcgggcgatg
     2941 agatcgtgcg cccgcgagta ggcctcctcg gcctccatgg tgcggccgag cagcgcggga
     3001 acggcgacgt tgtcgaccgc ggtcaggttc gacaacaggc tcgggaactg gaacacgaag
     3061 ccgacgtggc gggagcggaa ggtcgcgcgc tcgctttccg gcagcgtcca cacgtcggtg
     3121 ccgtcgagca gcagcttgcc ttcggtcggc atggtcaagg caccgagcat ggccatcagc
     3181 gtcgacttgc ccgaacctga tcggccgacg atcgagacga actcgccgcg gctgagctcg
     3241 agactgacgc cgcgcacggc ggcgtagccg ggagcgtcgc cgtagcgctt ggtcagcacc
     3301 tgcgccgaga gcgtcacgtc accgaccttc cgcctggatg agtgcatagg gcgccatccg
     3361 ccgcacccgc cacgccggca gaaaggcgcc gacgagaccg aggacggccg agaacacgat
     3421 cgccag
//
LOCUS       DATLJT010001678         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_638196,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001678 DATLJT010000000
VERSION     DATLJT010001678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638196"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VM910_38230"
     CDS             complement(<1..699)
                     /locus_tag="VM910_38230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HVI68373.1"
                     /translation="MLASADTSPDLDNGPRTEKSATTRMCAVSRTVRPIDELIRFVVS
                     PQGEVIADVKRKLPGRGLWISASRQAVAEAVRRHQFSRGFKREVRVAAALAADTEVLL
                     VRNAVEALAIAAKAGEVICGFSKVEAALRARQSGNAIGALIHAADGAPDGIRKLDALA
                     RQNAGIHGESAQFPTISALTSAELDLALGRLNVVHAALLAGPASKTFLSRSQILVRYR
                     SADDDKTAGIAARIS"
     gene            complement(723..2333)
                     /gene="nusA"
                     /locus_tag="VM910_38235"
     CDS             complement(723..2333)
                     /gene="nusA"
                     /locus_tag="VM910_38235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HVI68374.1"
                     /translation="MAVSANKLELLQIADAVAREKSIDRSIVISAMEDAIAKAARARY
                     GSETDVHAEIDAKKGELRLSRHMLVVEQVENSANQISLEDARRANPGAQIGDTIADTL
                     PPLEYGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDLG
                     RGEAIIRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEVP
                     EIYDGIVEIKAVARDPGSRAKIGVISRDSSVDPVGACVGMRGSRVQAVVNELQGEKID
                     IIPWSPDIATFVVNALAPAEVAKVVIDEDRERIEVVVPDTNNQLSLAIGRRGQNVRLA
                     SQLTGWDIDILTEQEESERRQADFENSTRVFMEALNVDEVVGQLLASEGFTSVEELAL
                     VDVKELAGIEGFDEETANELQNRAKEHLDQQEAELEAKRRELGVEDDMKAVPGVTSKM
                     LVKFGENDIKTVEDLAGCATDDLVGWTERKEGGEPTRHAGVLDGIEISRADAEAMIMQ
                     ARVKAGWITEADLAKEGTEAEPAEQPSA"
     gene            complement(2336..3079)
                     /gene="rimP"
                     /locus_tag="VM910_38240"
     CDS             complement(2336..3079)
                     /gene="rimP"
                     /locus_tag="VM910_38240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HVI68375.1"
                     /translation="MTDPSLGSMDAELLAEPRLVVEPGVAARVSAIASPVLQQMGYRL
                     VRVRISGEAGCTVQIMAERPDGSMQIDDCEAISRALSPVLDIADPIERAYRLEISSPG
                     IDRPLVRRSDFERHTGHLAKIEMTVAHHGRKRFRGTLGGVEGNAVRLTRDDAPEGEGV
                     DVLLALEDIGEAKLVLTDELIAESMRRGKAAERELKRNLGLEPPPPSHAKAHSKSRPP
                     KREAPKNTKQHRLAAEREKRGDFDPDRGD"
BASE COUNT          554 a         1159 c         1036 g          692 t
ORIGIN      
        1 cgaaatcctg gccgcaattc cggcagtctt gtcgtcatcg gccgaccggt atcggaccag
       61 gatctggctg cgcgacagga atgtcttgct cgccgggccc gcgagcagcg cagcatgtac
      121 cacatttaac cggcccaatg ccaaatccaa ttctgcggat gtcaacgcgc tgatggtcgg
      181 aaattgcgcg gattcaccat gaatcccggc attttgtcgc gccagggcat ccagcttgcg
      241 gattccgtcg ggcgccccgt cggcggcatg gatcaaggcc ccgatcgcgt tcccgctctg
      301 ccgggcccgg agagccgcct cgaccttgct gaagccgcaa atcacctcac cggccttggc
      361 ggcaatcgcc aaagcctcaa ccgcattgcg taccaacagg acctcggtgt cggctgcgag
      421 cgccgccgcc acgcggacct cgcgcttgaa acccctgctg aattggtgac gccgcaccgc
      481 ttccgcaact gcttgacgag acgcagaaat ccataatccc cggccgggga gcttgcgctt
      541 gacgtcggcg atcacctcgc cttggggcga gaccacgaag cggatcagtt cgtcgatcgg
      601 ccgtacggtg cggctgaccg cgcacatccg cgtcgtcgcg gacttttcgg tccgcggtcc
      661 gttgtcaaga tcagggctcg tatccgcgct ggccagcatc cgtgcggcat ctccatctga
      721 ccttaggccg aaggctgttc ggcgggctcg gcttccgtgc cctccttggc gagatcggcc
      781 tcggtgatcc agccggcctt gacgcgggcc tgcatgatca tggcttcggc gtcggcgcgt
      841 gagatttcga tcccgtcgag tacgccggca tgcctggtcg gctcgccgcc ctccttgcgc
      901 tcggtccagc cgaccagatc atcggtggcg cagcccgcga ggtcctcgac cgtcttgatg
      961 tcgttttcac cgaacttcac cagcatcttg gaggtgaccc ctggaaccgc cttcatgtcg
     1021 tcctcgacgc caagttccct tcgcttcgcc tcgagctcgg cctcctgctg gtcgagatgt
     1081 tccttggccc ggttctgcag ctcgttggcg gtttcctcgt cgaaaccttc gatgccggca
     1141 agttccttga cgtcaaccag cgccagctcc tccacagagg taaagccttc cgacgccagg
     1201 agctggccca ccacctcgtc gacattgagc gcctccatga acacgcgcgt ggagttctcg
     1261 aagtctgcct ggcggcgctc cgattcctcc tgctcggtca ggatgtcgat gtcccagccg
     1321 gtgagctgcg aggccagccg cacgttctgg ccgcgccggc cgatcgcaag cgaaagctgg
     1381 ttgttggtgt cgggcaccac gacctcgatc cgctcgcggt cttcgtcgat cacgaccttg
     1441 gcgacttcgg cgggcgccag cgcgttcacg acgaacgtcg cgatatccgg cgaccaggga
     1501 atgatgtcga tcttctcgcc ctgcagctcg ttgacgaccg cctgcacgcg cgaaccgcgc
     1561 atgccgacgc aggcgccgac cgggtcgacc gacgaatccc tcgagatcac gccgatcttg
     1621 gcgcgcgaac cgggatcgcg cgccactgcc ttgatctcga cgatgccgtc gtagatctcc
     1681 ggcacttcct gcgcgaaaag ctttgccatg aattgcggat gagtgcgcga caggaaaatc
     1741 tggggcccgc gggtctcgcg ccgcacgtcg aaaatatagg cgcgaacgcg gtcgccgttg
     1801 cggaacacct cccgcggcag catttcgtcg cggcggatga tggcttcgcc gcggccgaga
     1861 tcgacgatga cgctgccata ttcgacgcgt ttgacgatgc cgttgacgat gtcgccgata
     1921 cggtccttga attcctggta ctgccggtcg cgctcggcct cgcgcacctt ctgcacgatc
     1981 acctgcttgg cggattgcgc ggcgatgcgg ccgtattcca gcggcggcag cgtatcggcg
     2041 atggtgtcgc cgatctgtgc gcccggattg gcgcggcgtg cgtcttccag cgaaatctgg
     2101 ttggcggaat tttccacctg ctcgacgacc agcatgtggc gcgacaggcg aagctcgcct
     2161 ttcttggcgt cgatctcggc atgcacatcg gtctcgctgc catagcgggc gcgcgccgcc
     2221 ttcgcgatgg cgtcttccat cgccgagatc acgatggatc ggtcgatcga tttctcgcgc
     2281 gcgaccgcat ccgcaatctg cagcagttcg agtttgttgg cactgactgc catggctagt
     2341 ctcctctatc aggatcgaag tcgcctcgct tctctcgctc ggcggcgagg cggtgttgct
     2401 tggtattctt cggggcctct ctcttcggcg gcctcgattt ggaatgggct ttggcgtgcg
     2461 atggcggcgg cggctcgagc cccagattgc gcttcagctc gcgttcggcg gccttgccac
     2521 gccgcatcga ttcggcaatc aactcgtcgg tcagaaccag cttcgcctcg ccgatgtctt
     2581 ccagcgcaag cagcacgtca acgccctcgc cctcgggcgc gtcgtcgcgt gtcagccgca
     2641 ctgcgttgcc ttcgacgccc ccaagcgtgc cgcgaaatcg cttgcggccg tggtgggcaa
     2701 ccgtcatctc gattttcgcg agatgacctg tgtgacgttc gaaatcggag cggcgcacca
     2761 gcggccggtc gatgccgggc gacgaaattt caaggcgata ggcgcgttcg atcgggtcgg
     2821 cgatatcgag taccggcgac aacgcccgcg aaatcgcctc gcaatcgtca atctgcatcg
     2881 atccgtccgg ccgctccgcc atgatttgca cggtacaccc ggcctcgccc gaaatcctca
     2941 cccggaccag ccgatagccc atctgctgca ggaccgggct ggcgatcgcg gacacccgtg
     3001 ccgccacgcc cggctcgacc accaggcgcg gttcggccag gagttcggcg tccatggaac
     3061 ccagactcgg atcggtcata gtcagcgtca aattcgtcct agattcttgt tcggcgcgat
     3121 cttttcggaa aggcccgttg ccgcctttcc aaaattgcgc ccgtattggg ttgccggcat
     3181 cctttccgtc ggctccggcg gatcagccca gccataccgt gccaagtctt ggcaagtcgg
     3241 cccaggtaat aaaaaagagc gggtcccgag gggcccactc tcaatacgcg atcgtatgag
     3301 acttaagttg ccgccgatat agcccttttc ggagggtgcg gcaaggccgg caggcctgtt
     3361 cgaggaggca atttgcggcc taacccttcc ttcacctagg ccgcttagat tgcaggaaaa
     3421 ccgtccttca ccccggccca a
//
LOCUS       DATLJT010001679         4759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3393634,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001679 DATLJT010000000
VERSION     DATLJT010001679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3393634"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..828
                     /locus_tag="VM910_38245"
     CDS             316..828
                     /locus_tag="VM910_38245"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVI68376.1"
                     /translation="MASRSKKSLRAEQHRADVARARRRWIRQQGFLDTTRLVFIDETA
                     VTINMVRLRGRCPRGERLISHIPQGAWKTITFVAALRHNKMVAPMVIDGPIDGPAFVA
                     YVGQCLAPMLKCNDIVVMDNLPAHKVPGVKEVIEAFGATVQYLPQYSPDLNPIEMPFS
                     KFKAFLRKVS"
     gene            complement(905..1093)
                     /locus_tag="VM910_38250"
     CDS             complement(905..1093)
                     /locus_tag="VM910_38250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68377.1"
                     /translation="MTRRKPQSPKRDDPVTAADVIAFIQQVCLVPEGKLVGQPLVLQD
                     WQQELEQIPIIWTRSRHV"
     gene            complement(1059..1514)
                     /locus_tag="VM910_38255"
     CDS             complement(1059..1514)
                     /locus_tag="VM910_38255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68378.1"
                     /translation="MPRGRNRSPAALSVVSVVMPGQGRPEPPAELDALEQRAWREVIA
                     ALPAHWVDPAGQLILRRLAAQAAIAESLEVRIRDLRAQGQEAGKEAGALAARHGMASK
                     SVADLLAQLRAAPKSRTVPRAAGPRVLQAPEWRPWEIKGDQAQAAKPEA"
     gene            complement(1641..1838)
                     /locus_tag="VM910_38260"
     CDS             complement(1641..1838)
                     /locus_tag="VM910_38260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68379.1"
                     /translation="MTLATIVDHVEPHRGDVNRFWLGKLQSLCKSCHDSTKRLVEQRG
                     YLPDVGPDGWPLDPRHPVYNR"
     gene            complement(2187..3593)
                     /locus_tag="VM910_38265"
     CDS             complement(2187..3593)
                     /locus_tag="VM910_38265"
                     /inference="COORDINATES: protein motif:HMM:NF040238.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase family protein"
                     /protein_id="HVI68380.1"
                     /translation="MTVDFADQFEELTSQSSSTTLPSSCALEDLVSYAQGRSCIYLPC
                     KSSWPNASVDDRLPRMPVLDANGNPVLNKKGGVTTIPASMWLAQNRSVEALTWDPGEP
                     EFIRGRLAVDGGYVEKSGATTLNFYRPPPAIERGDSTQASRWVEHWRMLYPEDAEHII
                     AWLACRVQRPGEKINHALVLGGAPKIGKDTLLEAVVRTIGEWNFQNIKLNHLGQRNNG
                     FLKSLIVRLNEARDVGEQGTVDRYRLFDHMKDLLAAPPNTIRVNEKYINEYFILNRSG
                     MVITTNYRDALYLPPDDRRHYVAFSERRGEEFPAAFWNDFWGWYEAGGFAHVAALLNQ
                     YDLSNFDPKTEPVKTDAFWYMVNADRSGEFSEIADAIDRLKDKDGKRPDALTITQLIV
                     AAPELEWLRAVKMRRLMRRRLEDNGYAVVVNPNAKASDGMWVIDGRRQAIYARAALDL
                     KQRLTAAEQLQAGEATKP"
     gene            complement(3660..>4759)
                     /locus_tag="VM910_38270"
     CDS             complement(3660..>4759)
                     /locus_tag="VM910_38270"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HVI68381.1"
                     /translation="HDEITIEVPDNTVNKQDVLAVMLEPPAWAVGWPLGGKVHSGRIY
                     FEGPATAEPPAKTVESVESALDTFVAGAPELPNTKEVEQGAAEMFLANLDRNTAPLPA
                     LVSLPLDSSSRVSCPFHDDPNPSCSIYPDHYHCHACGAHGDRIDWLMRVEDMTRAEAL
                     DALYAWDAPSTQEQQQSEDEKRDFFRDIWNATQPLAGTIGERYLAETRGIDVSKLPPT
                     IKSVLRFHPNCVFGAGERHPCIVALMQDPLTDAVTGIHRIGLRQDNGAVLKIGRRALG
                     HMGVVKLWPGQRPARRRRRNRDRARRRYADSLSRRAADAGLVGGGARRARAPAGAAGD
                     RNAHSTCRPRRQQRRTERRHARPPSVDRGRA"
BASE COUNT          892 a         1471 c         1463 g          933 t
ORIGIN      
        1 acctgcccga ctggagacga cgcgatgtgg cgtaacaacg ggcgccgccg cctctacacc
       61 gaggcctatg tcgccagcct gatggccaag gcccgtagcg atctgcacgc gctgaatttc
      121 gagcagctga ccgagctcaa gcggctgcgc aacgagggtg gctgagctcc gcgccacgct
      181 gcaggaactg cgcgctgcca cgctggcccg tcagcacgcc caagccgagc tcaccagcct
      241 ttaccgggag cgcgcgattg cggaagcgac ggcttccggg cagccgccgc gcgctatggc
      301 ggtttctcga tcgacatggc atcacgttca aaaaaaagcc tgcgggcaga gcagcaccga
      361 gcagacgtgg cgcgagcgcg gcggcgctgg attcgacagc aaggctttct tgatacaacg
      421 cgactggtct ttatcgatga gaccgcggtc actatcaata tggtccgtct caggggacgt
      481 tgcccgcgtg gcgagcgact gatcagtcat attcctcaag gagcatggaa gacgatcaca
      541 ttcgtcgctg ccttgcggca taacaagatg gtggctccga tggtcatcga cggaccgatc
      601 gatggaccag ctttcgtggc ctatgtgggg caatgcctgg cgccgatgct caagtgcaac
      661 gacatcgtgg tgatggataa cctcccggct cacaaagttc ccggcgtgaa ggaggttatc
      721 gaagcttttg gcgcaacggt gcagtatctg ccgcagtact cgccggacct taatccgatc
      781 gagatgccgt tcagcaaatt caaagccttc ctgcgaaagg tctcctagcg gactgttcgt
      841 ggcctgtgcc gccggatcgg ctcgttcgtg ccaaccatca gtcgtgcaga atgtcgcaac
      901 tacttcagac atgccggcta cgcgtccata tgatcggaat ctgctctagt tcttgctgcc
      961 agtcctgcag caccagcggc tggccgacga gcttaccctc gggaacaaga cagacctgtt
     1021 ggataaacgc gatgacgtcg gcggcggtga ccggatcgtc acgcttcggg ctttgcggct
     1081 tgcgcctggt cacccttgat ctcccacgga cgccattcgg gagcctgcag gactcgcgga
     1141 cccgcggcac gcggtacagt gcgcgacttc ggcgcggccc gcaactgcgc gagcaaatcg
     1201 gcaacggatt tcgacgccat gccatgccgg gcagcaagcg cgccggcctc cttgccggcc
     1261 tcctgaccct gcgcacgcag atcgcgaatc cgaacctcaa ggctctccgc gatcgcggcc
     1321 tgggcagcca ggcggcggag gatcagctgc ccggcagggt cgacccaatg cgccggcaac
     1381 gcggcgatga cctcgcgcca agcccgctgt tcaagcgcgt ccagctccgc aggcggctcc
     1441 gggcgaccct gacccggcat gaccacagaa acgaccgaga gcgcggcagg ggagcgatta
     1501 cgaccacgag gcattggaaa cctccgcgtg gatgttaaca cgaggacccg aggcgaacgc
     1561 actttggttg tgccgcaggg ccgccgcgct ccgtgccggg gctcgcgcga aaattttcaa
     1621 tatcccccca cccccgactt tcagcgatta taaaccgggt gccgcggatc gagcggccag
     1681 ccatcgggac ccacgtcggg aagatagccg cgctgctcaa cgagccgctt cgtactgtcg
     1741 tgacaagact tgcacaacga ttgaagcttg ccgagccaga accgattgac gtctccgcga
     1801 tgcggctcga cgtgatcgac aatcgtagct agcgtcacga cacctcgctg aaggcagaac
     1861 gcgcacagtg gctcgatacg cagttgatgg ctccgtaaat gttgccaccg cgcgagcttg
     1921 taccagtgat cgtgtttttt cgtgcgcttc gtcatagagg gacacatccc cgttggtgat
     1981 attggtggtt tgacgggcag gcaaaacgat gacgaaggcg attggtgata ttggtggttc
     2041 tgacggcggg ggaacccaaa atggtcacca atttcaccaa ccaattggtg atgttggtag
     2101 ggtctataac ttctgtgcgt gaaaaaagcc agattatcta tcctgtaaaa gtttaaaacc
     2161 ttaccaatta caccaattcg accggctcac ggcttcgtgg cctcgccggc ctgtaattgc
     2221 tccgctgcgg tcaaccgctg tttcaggtct aatgcagcac gggcatagat tgcctgccgt
     2281 ctgccatcga taacccacat cccgtcgctt gccttagcgt taggattgac gacgacggcg
     2341 tagccgttgt cctcaagccg ccgccgcatg agccgccgca tttttacggc acgcaaccat
     2401 tcgagctcgg gggctgcaac gatcaattgc gtgatcgtca gcgcgtctgg ccgcttgccg
     2461 tccttatctt tcaaacggtc gatagcatcg gcaatctcac tgaattcacc actgcgatcg
     2521 gcgttgacca tgtaccagaa cgcgtccgtc ttcaccggct cagtcttggg gtcaaagttc
     2581 gataggtcat attggttgag cagcgcagcg acatgggcaa aaccgccggc ctcgtaccag
     2641 ccccaaaagt cgttccagaa tgcagccggg aactcctcgc cgcggcgctc ggagaacgcc
     2701 acgtagtgcc ggcggtcgtc gggcggcaga tagagcgcgt cgcggtaatt ggtggtgatg
     2761 accatcccgg agcgattcaa gatgaaatat tcgttgatgt acttttcgtt gacccggatc
     2821 gtgttgggcg gggccgcgag caggtccttc atgtgatcga atagccgata acggtcgacg
     2881 gtgccctgtt caccaacgtc gcgcgcctcg ttgagccgaa cgatcagcga tttgaggaaa
     2941 ccgttgttgc gttgaccgag gtggttgagc ttgatgttct gaaaattcca ctctccgatt
     3001 gtacgtacga ctgcttcgag cagcgtatcc ttgccgatct tgggcgcgcc gccgagcacg
     3061 agcgcgtggt tgatcttctc gcccggtcgc tgcacgcgac acgccaacca cgcgatgatg
     3121 tgttcggcgt cctcgggata gagcatgcgc cagtgctcga cccaacgcga tgcttgcgtc
     3181 gaatcaccgc gctcaatcgc aggcggcgga cggtagaagt tgagcgttgt cgcgcccgat
     3241 ttctcgacgt agccgccgtc aaccgccaag cggccgcgga tgaattccgg ctcgcccggg
     3301 tcccacgtca gcgcctcgac gctgcggttt tgcgccagcc acatgctcgc cgggatcgtc
     3361 gtcacgccgc cctttttatt gagcaccgga ttgccgttgg cgtcgagcac tggcatgcgc
     3421 ggcaatcgat cgtcgacgct ggcgtttggc cacgacgatt tgcacggcag atagatgcac
     3481 gaacggcctt gcgcgtagga tacaaggtct tcgagcgcgc atgatgacgg cagcgtcgtg
     3541 ctggatgact gcgacgttag ttcctcgaat tgatccgcga aatcgaccgt catgctttcc
     3601 tcccaagcac cacatcgttg aaatcccagc ccgcttgttt cgggatcagc ggcaccacct
     3661 tatgcccggc cacgatccac gcttggcggc cgcgcgtggc ggcgttctgt ccggcgttgt
     3721 tggcgtcgtg gtcgacaagt tgaatgagcg ttgcgatcgc cggcagcacc ggcaggcgcg
     3781 cgagcccgcc gcgcgccacc gccgaccagg ccggcgtcag cggcgcgccg cgataaggaa
     3841 tccgcgtagc ggcggcgagc acggtctcga ttccttcgcc gacgacgagc tggtcgctgc
     3901 cccggccaca gtttcaccac gcccatgtgg ccaagcgccc ggcggccgat cttgagtacc
     3961 gcgccgttgt cttggcgcag gccgatgcga tgaattccgg tcactgcgtc ggtgagcggg
     4021 tcttgcatca gcgcgacgat gcaagggtgc cgctcgccag cgccgaagac acaattcggg
     4081 tgaaagcgca gcacactctt gatcgtgggc ggcagtttgc tcacgtcgat accgcgggtt
     4141 tcggcgagat aacgctcgcc gatggtgcca gcgagcggtt gcgtcgcgtt ccagatgtcg
     4201 cggaagaagt cgcgcttttc gtcctcgctc tgttgctgct cctgcgtcga cggggcgtcc
     4261 caggcgtaga gcgcgtccaa agcttcggca cgggtcatgt cttcgacccg catgagccaa
     4321 tcgatccggt caccatgcgc gccgcaggcg tggcagtgat agtgatcggg atagatgctg
     4381 catgacggat tcggatcgtc gtggaacggg cacgacaccc ggctgctgga atccagtggt
     4441 agtgaaacca acgcaggcaa tggcgcggtg ttgcgatcca aattggcgag aaacatctcc
     4501 gccgcgcctt gttcgacctc tttcgtgttc ggcagttcgg gggcgccggc gacgaacgtg
     4561 tcgagtgcgg actcgacgga ttcgacggtt ttggccggcg gttcggccgt cgccggccct
     4621 tcgaagtaga ttcgcccact atgcaccttg ccgccgagcg gccagcccac ggcccatgcg
     4681 ggcggctcca acatcaccgc gagcacgtcc tgcttgttga cggtgttgtc cggcacttcg
     4741 atcgtgatct cgtcgtggc
//
LOCUS       DATLJT010001680         5452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3625848,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001680 DATLJT010000000
VERSION     DATLJT010001680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625848"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VM910_38275"
     CDS             complement(<1..646)
                     /locus_tag="VM910_38275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68382.1"
                     /translation="MKRFEGKTILVTGAGSGIGAACVRRLFAEGASIGAADVRKEDVD
                     QVVSEFGNSERIFGLGVDVSDRDQVAAFVSGAIRRFGKLSGLVNSAGIRGVGNVLDTD
                     PEIWRKVLSVNLEGTFNMCQAFARAVKEAQTPAAIVNLSSAAGIRGVPNRLPYSASKY
                     GVVGITQTIALELGPLGVRVNAVVPGMIRTPMTAVMFQDPENVKRIRAAHPIGRE"
     gene            complement(765..1232)
                     /locus_tag="VM910_38280"
     CDS             complement(765..1232)
                     /locus_tag="VM910_38280"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HVI68383.1"
                     /translation="MSAEQMVPQMSTQFPGSDGTEASMTCASLDSLADKQVLYLTTVG
                     RRTGLPREIEIWFVVWRERLYLFAETREAAGWVKNIRRNPKVMVRIGEWRTDATARVL
                     DPHTDRKLWGQVAAIAARKYGWGDGLPVEITHFPHALPIPADETLKEIEGETA"
     gene            complement(1452..2798)
                     /locus_tag="VM910_38285"
     CDS             complement(1452..2798)
                     /locus_tag="VM910_38285"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68384.1"
                     /translation="MIADKNEGAIAPAVDEIKAAGGGTAGFLVDVADRGQVQAFMTKV
                     VAAHGRVDILVNNAGITRYRPFLTMSDEDWNLVLDVDLKGVFFCVQAAAPQMIRQAYG
                     KIVNISSALGTGTRPHNTAGSPAVSSAYASAKAAVIQLTKTLARELGPHGINVNCVAP
                     GTFLTPLTAATRTPEQVAEHIAHRTKTVVLNRLGSLEELANAVLFLASRRSELYRWPY
                     SVCRWRANGSHVTSRGGGLLMLTLYYSPCACSLAPHIALEEAGATYALQLVSIPKGEQ
                     QASEYLNVNPRGRIPALRTAEGVLTENVAILTYIARSFPQARLLPEDPIDMARCLSHM
                     AWLSNTVHPAFTHIVRPGRFATDEAAHANLKATGRENAWKLLQELDSFLAGSEWVLGS
                     CHSVADPYTLVIYGWGKGHSMPVEQLANYTAFKERMLRRPAVRRALEREESRLLDG"
     gene            complement(2907..3713)
                     /locus_tag="VM910_38290"
     CDS             complement(2907..3713)
                     /locus_tag="VM910_38290"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HVI68385.1"
                     /translation="MRLFYQSFGVSRRSRDGRYGQVLKTILDRAAAPGTEISIHGLSP
                     HRAIAEQYRYLEFLDKAEVVDNGLRAEKEGYNAFVLGNIFDPGLHELRELLNIPVLGL
                     CESSVHVARLMGATFSLINVNPKFTHRIVESIKLQGLAARLVSVDQMRVERPGVFDLA
                     FGDDQAKAEIVRQFAECARRSLEQGAEVLIPAGGSLMAVLADAGVHQIGSAPVLNGLI
                     AVIKIAELAVAMRQLTGQFTSKHLIYAPPSGKLLADVWEAYGPHVYPGAT"
     gene            complement(3846..4208)
                     /locus_tag="VM910_38295"
     CDS             complement(3846..4208)
                     /locus_tag="VM910_38295"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HVI68386.1"
                     /translation="MANFDADTLRELRDVRELRIRTGKHPKTAVVIWIVVADEEVFAR
                     SWLGGRGRWYRDLAAGGPATLEFAGRRLEVQASPASDDTSIARASAEFLRKYQPSSHA
                     REMVRSEILSTTLRLEPR"
     gene            4335..5240
                     /locus_tag="VM910_38300"
     CDS             4335..5240
                     /locus_tag="VM910_38300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI68387.1"
                     /translation="MQRGNLNDLLVFRAVATERSFTRAAAKLGVSQSALSHTIRGLEE
                     RLGLRLLTRTTRSVAPTEAGERLLHTVGPRFEEIEAELEALSELREKPAGTIRITATD
                     YAADTILWPKLAKLLRQYPDIKVEISIDYGLVDIVAERFDAGVRSGEQVAKDMIAVRI
                     GPDMRSAVVGAPSYFKEHPEPRRPQELLRHNCINLRLPTRGGLYAWELEKDGRELKVH
                     VEGQLVFNGTFQMLNAAVDGFGLAYVPEDVARPHLAKGRLRRVLDDWCPPYSGYHLYY
                     PSRRQSTPAFALLVEALRYRAPRSA"
BASE COUNT         1021 a         1759 c         1621 g         1051 t
ORIGIN      
        1 cctcacgacc gatgggatga gccgcgcgaa tccgcttcac gttttcgggg tcctggaaca
       61 tgaccgccgt catcggcgtg cggatcatcc ctggcacaac ggcattgacg cggacgccga
      121 gcggtccgag ttccaatgcg atcgtctggg tgatccccac taccccgtac ttcgacgcgg
      181 aataggggag gcgattgggc acgcccctga tcccggccgc agagctaagg ttcacgattg
      241 ccgcaggcgt ttgcgcttcc ttcacggcac gagcgaaagc ctggcacatg ttgaacgtgc
      301 cttcgaggtt gaccgacagg accttccgcc aaatttcggg gtcggtatcc agaacgttgc
      361 cgactccccg gatgccagcg ctgttgacca gcccagagag ttttccgaac cgccgaatcg
      421 ctccggacac gaacgcggcc acttggtcac ggtccgaaac gtcaacgccg aggccaaaga
      481 tcctctcgct gttcccgaat tccgacacga cctgatcgac gtcttctttc cgtacgtctg
      541 cggcgccgat cgatgcacct tctgcaaata atcgcctgac gcaagccgcg ccgataccgc
      601 tgccggcgcc agttactaag atcgtcttgc cttcaaagcg tttcattcaa acaccctttc
      661 cttcagataa ccagtacgcc aacgcaatgg ctactgagac ttcttcgttt ccgacctttg
      721 caatcgcgcc cacatcttta acggttgcat ccgcctgctg agccttatgc cgtctctcct
      781 tcaatttcct tgagtgtctc gtcagccgga atggggagcg cgtgagggaa gtgagtgatt
      841 tccaccggca gcccgtcgcc ccacccatat ttgcgagcgg cgattgcagc gacctggccc
      901 cacagtttgc gatcggtgtg agggtcgagc acacgcgccg ttgcgtcagt gcgccattct
      961 ccgatgcgaa ccataacctt ggggttgcgc cttatgttct tgacccatcc ggccgcttca
     1021 cgtgtttcgg caaacaggta gagacgctcg cgccagacca cgaaccaaat ctcgatctcg
     1081 cggggcagac ccgtcctacg cccgaccgtg gtcagataaa ggacctgctt gtccgcaaga
     1141 ctgtcgagac tcgcgcaggt catcgaggct tccgttccat ccgaaccggg aaactgggtc
     1201 gacatctgcg gcaccatctg ctccgcggac atttccactg acggtgaatt gaatttagtg
     1261 ggtgcaaccc gcggcgatta gctggcggca ttcgcatgga cttatgaact gcatttctga
     1321 atggccgccg aaccacccgc tccccgtggc gttctagcaa tgccgagagc ctgagtttcg
     1381 tgacatagct gccgtaagtg cgagccacac cgccaagaag cagcgggaga gtgggttagg
     1441 gtccgatctg gtcagccgtc gagcaaccgg ctctcctcgc gctcgagcgc ccggcggacg
     1501 gccgggcgtc gcagcatgcg ttccttgaag gcggtataat tcgcgagctg ttcgaccggc
     1561 atgctgtgcc ctttgcccca gccgtagatc acaagtgtgt aggggtcggc caccgaatga
     1621 cacgagccca gaacccattc gctgccggcg aggaacgagt cgagctcctg cagcaatttc
     1681 caggcattct cgcgcccggt ggccttcagg ttcgcgtggg cggcttcatc ggtggcgaag
     1741 cgtcccgggc gaacgatgtg ggtgaaggcc gggtgcactg tattcgacag ccaggccatg
     1801 tgcgagaggc agcgcgccat gtcgatcggg tcttccggca acagccgcgc ctgcggaaac
     1861 gagcgggcga tgtaggtcag gatcgcgaca ttctcggtca gcacgccctc ggcggtgcgc
     1921 agcgccggta ttctgccgcg cggattgaca ttcagatact cggaagcctg ttgctcgcct
     1981 ttcgggatcg acaccagctg cagcgcatag gttgcgccgg cttcttcgag cgcgatgtgc
     2041 ggcgccagcg agcaggcgca gggagaataa tagagtgtca gcatcagaag tcctcctcct
     2101 cgactggtca catgcgatcc gttcgcccgc catcgacata cagagtatgg ccagcgatat
     2161 aactcgcttc gtctggaagc gaggaacagc acagcgttcg cgagttcttc gagcgatccc
     2221 aggcgattca acacgacggt cttcgtcctg tgcgcgatgt gctctgccac ttgctccggc
     2281 gtgcgggtcg ctgcggtcag cggcgtcagg aaggtgccgg gtgcgacgca attcacattg
     2341 ataccgtgcg gtccaagctc acgcgccagc gtctttgtaa gctgaatcac cgccgccttg
     2401 gcggacgcgt aagccgagct gactgccggc gaccccgcgg tattgtgcgg ccttgtgccg
     2461 gtgcctagag ccgaagagat gttgacgatc ttcccgtagg cttgccggat catctgcggc
     2521 gcggccgcct ggacacaaaa gaacacgccc ttcagatcca catccagcac caggttccaa
     2581 tcttcatcgg acatcgtcag gaacggccgg taacgcgtga tcccggcatt gttcaccagg
     2641 atgtccacgc ggccatgtgc ggcgacaacc ttggtcatga aggcctgcac ctgcccgcga
     2701 tcggcaacat cgacaagaaa tccggcggtg ccaccgcctg cggccttgat ttcgtcgacg
     2761 gcgggggcta tcgcgccctc gttcttatcg gcgatgatta cgaacgctct ctcgctggca
     2821 agcgttgcgc aatcgccttg ccgatgcccg agccggagcg gtcacgatcg ccacttttcc
     2881 cttcaagcgc atcggcttct cctccgttat gtggctccgg gatagacatg cgggccgtaa
     2941 gcctcccaaa catcggcgag caatttcccc gacggcggcg catagatcag gtgcttgctg
     3001 gtaaactggc cggtgagctg gcgcatcgcg accgcaagct cggcgatctt gatcaccgcg
     3061 atcagcccgt tgagaaccgg ggcggaaccg atttgatgca cgccagcgtc ggccagcaca
     3121 gccattagac tccctcccgc cgggatgagc acttcagcgc cttgttcaag gctgcgcctt
     3181 gcacattcgg caaactgcct gacgatctcg gctttcgcct ggtcatcgcc aaacgcgagg
     3241 tcaaagacgc ccggccgctc gactctcatc tggtccaccg agaccaagcg cgcggcgagg
     3301 ccctgcagct tgatgctctc gacgatgcga tgcgtgaact tcggattgac gttgataagc
     3361 gagaaggtcg cgcccatcag gcgggcaacg tgaaccgagg attcgcacag accaagcacc
     3421 gggatattga gcaattcccg cagctcgtgc agcccgggat cgaagatgtt gccgagtacg
     3481 aaggcattgt aaccttcttt ctcggcccgc agcccgttgt ccacgacctc ggccttgtcc
     3541 aagaattcaa ggtagcggta ctgctcggct atcgcccggt gcgggcttag gccgtgtatc
     3601 gaaatctcgg ttcccggcgc ggctgcccga tcaaggatgg tctttagaac ttggccatag
     3661 cggccatcgc gtgaccgacg cgacacaccg aagctttgat agaacaagcg catcacccac
     3721 ccctcctcga tgccgtcctg gggacatgag tgtttgtacc ccggatcccg cggtgaacgg
     3781 tcccgccgac ggccttggct aggtggtcgt gagaattggc ttattccggg tgttgaggcg
     3841 ggcggtcagc gcggttcgag ccgcagcgtg gtggagagga tttcagagcg caccatctcc
     3901 cgtgcatgcg agctcggctg gtatttccgc aggaactcgg cgctcgcccg agcgatcgag
     3961 gtgtcgtcgc tcgccgggga cgcctgtacc tcgagccggc gcccggcgaa ctccaatgtg
     4021 gcgggcccac ccgccgcgag gtccctgtac caacgtcccc tgccgccaag ccacgaacgc
     4081 gcgaagacct cttcgtcggc tacgacgatc cagatcacca ccgcggtctt cgggtgcttc
     4141 ccggtccgga tcctcaactc ccgcacatcg cgtagttccc gcagcgtgtc cgcgtcgaaa
     4201 tttgccatgc cgtcatcccc cgtccgccgc ggcggcccac cctcgatcgc gccgccgccg
     4261 gccaacatct agcagggcgc gccggtcgcg attagtccct ataattggca tggagttatg
     4321 agcggcagcc ataaatgcag cgcggcaatt tgaatgacct cttggtcttt cgggcggtcg
     4381 caaccgagcg cagtttcacg cgggctgcag cgaagctggg cgtatcccaa tcggcgctca
     4441 gtcacaccat tcgcggcttg gaagaacggc tcggcctacg gctattgacc cgcaccacgc
     4501 gcagcgtcgc cccgacggaa gcgggcgaac gcctgctcca caccgtcggc ccgcggttcg
     4561 aagagatcga ggccgagctc gaggcgctga gcgagcttcg cgagaaaccc gccggcacca
     4621 tccgcatcac ggcaacggac tatgcggccg acacgatcct ctggccgaaa ctcgcgaagc
     4681 tcctgcggca atatcccgac atcaaggtcg agattagcat tgactatggt ctcgtcgaca
     4741 tcgtcgcgga gcgtttcgat gcgggcgtgc gcagcggcga gcaggtggcg aaggacatga
     4801 tcgccgtgcg catcgggccc gacatgcgca gcgcggtcgt cggggcaccg tcgtatttca
     4861 aggaacaccc ggaaccgagg cggccacagg agctcctccg tcacaattgc atcaatctgc
     4921 gcctaccgac gcgtggcggg ttgtatgcct gggagctcga gaaggatggc cgcgagctga
     4981 aggtgcatgt cgaaggtcag ctcgtcttca acggcacgtt ccagatgctc aatgccgcgg
     5041 tcgacgggtt cggcctcgcc tatgtgccgg aggacgtggc gcggccccat cttgccaagg
     5101 gccgcctcag acgggtgctc gacgactggt gcccgccata ttccggctat cacctctatt
     5161 acccgagccg tcgtcaatcg accccagcat tcgccttgct agtcgaggcg ctccgctacc
     5221 gtgcgcctcg gtcagcttag gatgcgagac ttagcgtcgg gaatcgagca aaggacgttc
     5281 cccaatcacc ctttcaggcc tccactacaa aaccgcggac gaccgctatg ggtcaaaatg
     5341 agaaggatcg gcatcgccac agcatttctc ctgaacctcc aatagcggaa atgtttgcgg
     5401 acacgtcggc actctgccgc gcgatcccag ctaccccttt ttggcagagg cc
//
LOCUS       DATLJT010001681         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4082965,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001681 DATLJT010000000
VERSION     DATLJT010001681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4082965"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..452)
                     /locus_tag="VM910_38305"
                     /pseudo
     CDS             complement(61..452)
                     /locus_tag="VM910_38305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
     gene            561..1412
                     /locus_tag="VM910_38310"
     CDS             561..1412
                     /locus_tag="VM910_38310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVI68388.1"
                     /translation="MKALDLEAVQAFVLTADLKSFTRAAEAMDTTQSAVSLRIKRLED
                     SLGRRLLERTPRLVRLSSDGSAFLEAARNLVAAHHGAISSFGVEQRRLVVGISHHIVG
                     AELPMLLKRLNSAEPSLVLEMRVATSREVLDEFDRGKIDAGIVFRHDDRRRDGETVLE
                     ETFGWMAAPDFEHRAGEPLRLATQAEPCSVRSMAVDALDGAGIAWTEVFVGGGVATTG
                     AAVSAGLAVAALGRRVAPPGTIDVGPRLGLPPLPSRDVVLLSAAADPQTRASLKALAA
                     TIRATAN"
     gene            complement(1447..1863)
                     /locus_tag="VM910_38315"
     CDS             complement(1447..1863)
                     /locus_tag="VM910_38315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HVI68389.1"
                     /translation="MSDNAAPRPISEIASFHAHVYYDPASTRAEAERLRMWIGERFSV
                     TLGRWHDVKVGPHDQAMYQVAFATEVFAELVPWLMLNHGNLSILVHPNTTNPRRDHLA
                     DPLWIGAPLALHGDKLPEHAEGEQAPAPNTQTALRP"
     gene            1962..2447
                     /locus_tag="VM910_38320"
     CDS             1962..2447
                     /locus_tag="VM910_38320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVI68390.1"
                     /translation="MDMLVKLYDLPDSRTLLERLAKAGISVRRALAPEKHKVTAWIRD
                     NFSEGWVSETEVAFSRQPVSCFIAVKDGRITGFACHDATCRNFFGPTGVTPKARKSGI
                     GTALLLACLEDMKQQGFGYAIIGGVGPADYYAKAVGAVLIEGSEIGVYRGLLGASRFA
                     D"
     gene            complement(2471..3817)
                     /locus_tag="VM910_38325"
     CDS             complement(2471..3817)
                     /locus_tag="VM910_38325"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HVI68391.1"
                     /translation="MLHKPPVTQILARFIAATRWDDIPQQVRHQAKRSLINFFAVALA
                     GCRTQPVEIALRSLAEFSGGKQAAVIGRNERIDALSAAFLNAASANVHDFCDTHLRTV
                     IHPTAPVAPALLALAEFRQVSGKDALLALVLGNDIQARIGLAISPQHYNKGWHITSTC
                     GVFGAAAAVGKLLGLGEGALVSALGIASTQSSGLCECLGMPAKSVSVGNAARNGLWSA
                     LLAEKDFDGPAEPLTGRQGFYSAMGDTPDLSSITDRLGEDFEIMATSYKPYPCGFVIH
                     PVLDCVLEWRREHSATQVTRVVVRGNPSLAHRTDRPDVSTGRESQVSVQHAVAAALVF
                     GAAGLEQFTDACVGDPRVQTLRNKVEVLRDEAFSTIAASVEITTGDGKTHPLTQAAAR
                     GSDVNPMSDRDLERKLRTAAAGWNEEYDVKPLIDAIWSLDESADFSRLAALAVPRA"
     gene            3967..>4610
                     /locus_tag="VM910_38330"
     CDS             3967..>4610
                     /locus_tag="VM910_38330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68392.1"
                     /translation="MPTRKLAIALALAVASAVIAGARADDYPSRPIRLIVPFAAGGAA
                     DSVARILGKRVGEALRQTIVVEDRGGGGGTIGTELVKKSDPDGYTLLLGQSGPISINP
                     GIYTKLSYDPEKDFAPISMTTAYPYIVVVNPALGVKTLAELVALAKSKPGELNYGTAG
                     VGVSNHLVTELFDDKAGIRMTHIPYRGTSLAVADLLAGQVQVVFADPVSALAHVR"
BASE COUNT          840 a         1420 c         1480 g          870 t
ORIGIN      
        1 cagccgatgg gcgtagtggg cgatgatcat gaggtttctc cttggataag gcgatcgtcg
       61 tcagctcttg gcggtcgcgt attgcgcgac gccattgccg aacgaccagt tttcgggaag
      121 gacttcgacg agattgatga gaacgtcttc gggccgcacg ccgggatcgg tcgaaagatt
      181 cgcgacgatc tgccgataaa gcgccttctt cctttcgacc gtgcgcgtgt tgttggcggt
      241 gatctggatc atgaccagat catcgctgcg cgcgatgccg agatagctct tgctgtagct
      301 gaaatccgcg tcgtcgtgct cggtgaagtc atgaagtgat cctcctcagg cacgtcgaag
      361 ctggtgagca tcgcggaata gattccgtcg aggatggctt tgcggaaggc tggcggcttg
      421 ccgcgccgaa gcgaaacgcg aaccaaaggc atggtcggcc tcctgtgctt gtgatgtgcg
      481 gaagagatag ctggcgcttg accatttcga aaccgtatta aatactattt caatgatatg
      541 ggattgaaat ggatgtagcg atgaaggcgc tggatcttga ggcggtacaa gcctttgttc
      601 tgacggcgga tttgaagagt ttcacacgcg cggccgaggc gatggacacc actcaatccg
      661 cggtcagcct gaggatcaaa cggctggagg attcgcttgg ccggcggctg ttggagcgaa
      721 cgccgcggct ggtccggctt tcttcggacg gcagcgcgtt cctggaagcg gcacgcaatc
      781 tcgtcgccgc ccatcacggc gcgatcagtt cgttcggcgt cgagcagcgg cggctcgtgg
      841 tcggcatcag ccaccacatc gtgggtgccg agttgccgat gctgttgaag cgcttgaaca
      901 gcgccgagcc ctcactcgtg ctggaaatgc gcgttgcgac ttcgcgcgag gtgctcgatg
      961 aattcgatcg cggcaagatc gatgcgggaa tcgtctttcg tcacgacgac cgccgccgcg
     1021 acggcgaaac cgttctggaa gagacgttcg gctggatggc cgcgcccgat ttcgaacatc
     1081 gcgcgggtga gccgttgcgg ctggcgacgc aggccgagcc ctgcagcgtg cgcagcatgg
     1141 ccgtcgatgc gctggacggc gcggggatcg cctggaccga ggttttcgtc ggcggcggtg
     1201 ttgcgaccac gggagccgca gtctctgccg gtcttgccgt tgccgcgctc ggccgccgcg
     1261 tggcgccgcc gggaacgatc gatgttggcc cccgactcgg gctgccgccg cttccctcgc
     1321 gcgacgtggt gctgctttcc gccgctgccg acccgcagac cagggcgtcg ttgaaagcgc
     1381 tcgcggcgac gatccgcgct acggcaaatt gaggatccca tctttgcgcg gcgcagcgaa
     1441 gccggctcaa ggccgaagcg cggtttgggt gttcggcgcc ggggcctgct cgccctcggc
     1501 atgttcgggc agcttgtcgc catgcagcgc gagcggcgca ccgatccaga gcggatcggc
     1561 aagatgatcg cggcgcggat tcgtcgtgtt cggatggaca aggatgctca aattgccgtg
     1621 gttgagcatc agccagggca cgagttcggc aaacacctcc gttgcaaagg ccacttgata
     1681 cattgcctga tcgtgcggac cgactttgac atcatgccag cgcccgagcg ttacagaaaa
     1741 gcgttcgccg atccacatgc gcaaccgctc ggcctcggct cgcgtgctgg cgggatcata
     1801 atagacgtgg gcgtggaagc ttgcgatttc gctgatcggc cgcggtgccg cgttgtcgct
     1861 catctccata gccccttggt gcaggccctc atccctttcc ggatttgtgg accagcgcga
     1921 atatgttagc aagatcgtat tgccgcagga gctgccaccc catggacatg ctggtcaagc
     1981 tttacgatct gcctgactcc cgcaccttgc tggagcggct ggccaaagcc ggcatcagcg
     2041 tgcggcgcgc gctcgcaccc gagaaacaca aggtcacggc gtggatccgc gacaacttct
     2101 ccgagggttg ggtcagcgaa accgaggtgg cgttcagccg gcagcccgtg tcctgcttca
     2161 tcgcagtcaa ggacggccgg atcaccggct ttgcctgcca tgacgccacg tgccgcaact
     2221 ttttcggacc caccggtgtg acgccgaagg cgaggaagag cggcatcggg acggccctgc
     2281 tgctcgcctg tctcgaggac atgaagcagc aaggtttcgg ctacgccatc atcggcggcg
     2341 tgggacctgc cgactattac gcaaaggcgg tcggcgcggt gttgatcgaa ggatccgaga
     2401 tcggcgtata tcgaggcctg ttgggcgcct cgcgattcgc agactgaaac gggcgctgcc
     2461 ggccctttgc tcaggcccgc ggtactgcga gcgccgcgag gcgggagaaa tcggcgctct
     2521 catcaagcga ccagatcgcg tcgatcagcg gctttacgtc atattcctca ttccacccgg
     2581 cagcagcggt ccgcagcttc cgctccagat cccgatcgct catcggattg acgtcgctgc
     2641 cgcgggccgc ggcttgcgtg agcggatggg tctttccatc ccctgttgtg atctcgacac
     2701 tcgccgcaat ggtcgagaaa gcttcgtcgc gcagcacctc gaccttgttc cgcaacgtct
     2761 gcacgcgcgg atcgcccaca caagcatcgg tgaattgctc cagccccgca gcgccaaata
     2821 ccagcgcagc ggcgaccgca tgttgcacgc tgacctgtga ttcgcgcccg gtcgagacgt
     2881 ccggacgatc ggtgcgatgc gcgagcgacg gattgccgcg caccaccacg cgcgtcacct
     2941 gggtcgcaga atgctcgcgc cgccattcca gcacgcagtc gagcacggga tggatcacga
     3001 agccgcaagg atagggcttg taagaggtcg ccatgatctc aaagtcctcg cccaggcgat
     3061 cggtgatcga cgacagatcc ggcgtatcgc ccatggcact gtagaatccc tgccgtcctg
     3121 tcagaggttc tgcagggcca tcaaaatcct tctccgcaag tagcgccgac cacagcccat
     3181 tgcgcgcagc gtttcccacg ctgacactct ttgccggcat gcccaggcac tcgcataggc
     3241 cggatgattg tgtggaggcg atgccgagcg cggataccag cgcgccttca cccaaaccaa
     3301 gcagcttgcc gacggcagcg gccgcgccga acacgccgca agtcgaggtg atatgccagc
     3361 ccttgttgta gtgctgcggc gagatcgcaa gcccgatgcg tgcctggata tcgttgccga
     3421 gcaccaaggc aagcaatgcg tctttcccgc tcacttgcct gaactccgca agcgcgagca
     3481 gcgccggcgc gaccggcgcg gtcggatgga tcacggtgcg caaatgggtg tcgcagaaat
     3541 cgtggacatt ggcggacgcg gcgttgagaa aggccgcgct cagcgcatcg attcgctcgt
     3601 tgcggccaat caccgcggct tgttttccgc cggaaaactc agccagtgag cgaagtgcga
     3661 tttcaaccgg ctgcgtgcgg caacccgcca gcgccacggc aaagaaattg atcagggaac
     3721 gcttggcctg atgccgaacc tgctgcggga tgtcatccca acgcgtcgcg gcaatgaagc
     3781 gcgcaagaat ttgcgtcacc ggcggcttgt gcaacatggt tcctcccgaa tgggccgcca
     3841 gcatccgacg taagacggaa aagcgcaacg gcgcgaacca tggaccggat aggcattttc
     3901 gccttattgt gcaccgagag tcacaggccc gcgatcaagc cgggcgcatt tccgggagag
     3961 acaagcatgc caacgcgaaa gctcgccatt gcgctggccc ttgccgttgc gagcgccgtc
     4021 atcgcgggcg cgcgggccga cgactacccc tcgcgaccga tccggctgat cgtgccgttc
     4081 gccgcgggcg gcgccgcgga ttcggttgcc cgtatcctcg gcaagcgcgt gggcgaagcg
     4141 cttcgccaga ccatcgtggt cgaggatcgc ggcggtggcg gcggcaccat cggtaccgaa
     4201 ctcgtcaaga aatccgatcc ggatggatat acgttgttgc tcggtcaatc ggggccgatc
     4261 tcgatcaatc cgggcatcta cacaaaactg tcttacgatc cggagaagga ttttgcgccg
     4321 atctcgatga cgaccgccta cccctatatc gtggtggtca acccggcgct cggcgtgaag
     4381 acgcttgcgg aactggttgc actcgccaag agcaagcccg gtgaattgaa ctacgggacg
     4441 gccggtgtcg gcgtctccaa tcacctggtc accgagttgt tcgacgacaa ggccggaatc
     4501 aggatgaccc acatccccta tcgcgggact tcgctggcgg tcgccgatct cctcgcgggg
     4561 caggtgcagg ttgtgtttgc ggaccccgtg tcggcactcg cacatgttcg
//
LOCUS       DATLJT010001682         2409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_864403,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001682 DATLJT010000000
VERSION     DATLJT010001682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864403"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1023)
                     /locus_tag="VM910_38335"
     CDS             complement(55..1023)
                     /locus_tag="VM910_38335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVI68393.1"
                     /translation="MTFAPAALAAWEPSQRYPDPAVQSLDPSFNKCRLALAGVERLAT
                     GCRFNEGAVYFGDARCLLWSDIPNNRILRWDEETGRISIFRQPSNHANGNTRDRQGRL
                     ITCEHSGRRITRTEYDGAVTVLIDRFDGKPLNGTNDVVVKSDDTIWFTDPPSGISGNY
                     LGVEAKPELPFDVYRLDPKSGLATVVTTGLVKRPNGLAFSADESKLYVIESAPEGRSI
                     YVFDVVDGGVKLANGRMFINCEKEETPDGFRVDVDGNLWCGWGMSLALDGVKVFNPAG
                     KAIGFIALPERAANVCFGGRYRNRLFMAASHSIYSLYVNTQGVAGG"
     gene            complement(1192..1536)
                     /locus_tag="VM910_38340"
     CDS             complement(1192..1536)
                     /locus_tag="VM910_38340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68394.1"
                     /translation="MRRRDFITLLGGAATTWPLAARAQQAERMRKVGLLMGPSDPDAR
                     ARVKALRQRLGELGWLEDRNTRIDVVWGAADADHVRADAAELMRESPDVIVTNGPVVP
                     DRLLAIADEVIE"
     gene            complement(1537..>2409)
                     /locus_tag="VM910_38345"
     CDS             complement(1537..>2409)
                     /locus_tag="VM910_38345"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68395.1"
                     /translation="FAENDRDAQAYTTAFRQALEKLGWAAGRNVRIDYRWGGAEPERI
                     RTYAIELVGLKPDVILAVTAQALQPLQQVTSSIPIVFTQMGDPIGSGFVTSMAHPGGN
                     ITGFTPAEFSMQGKRLEMLKEVAPQITRVAVLFNPEQIPQAGMLRAVEAVAPSVGVQL
                     TAAAVRDAAEIERAIDQFAREPNGGLIVLPSGPTIVHRDLILALAARYRLPAAYQYRQ
                     FVTAGGLMSYGNDLADQYRQAASYVDRILRGEKAGDLPVQAPTKFELVINLNRQSARP
                     RNAADAARPRRRGD"
BASE COUNT          437 a          782 c          734 g          456 t
ORIGIN      
        1 tcgttagccc gacaacaggc tgttgttttc cggcagaagc caactgagat gtgcttaccc
       61 gccagccacg ccctgcgtgt tcacgtagag cgaatagatc gagtggctcg ccgccatgaa
      121 cagccggttg cggtagcggc cgccgaagca gacgttggcg gcgcgctcgg gcagcgctat
      181 gaagccgatc gccttgccag ccggattgaa caccttgacg ccgtcgagcg ccaggctcat
      241 gccccaaccg caccacaaat tgccgtcgac gtcgacccga aagccgtcgg gcgtttcttc
      301 tttttcgcag ttgatgaaca tgcggccatt ggcgagcttc actccgccgt cgaccacgtc
      361 gaagacgtag attgagcgtc cctcaggcgc gctctcgatg acatagagct tcgattcgtc
      421 ggccgagaag gcgagcccgt tcggccgctt gactaggccg gtggtcacca ccgtggcgag
      481 cccactcttg ggatcgagcc ggtacacgtc gaagggcaat tccggcttgg cctccacgcc
      541 cagataattg ccggaaatgc ccgagggcgg atcggtgaac cagatggtgt cgtcggactt
      601 caccaccacg tcgttcgtgc cattgagcgg cttgccgtcg aatcgatcga tcagcaccgt
      661 gacggcgccg tcatactcgg tgcgcgtgat gcgacggcct gaatgctcgc aggtgatgag
      721 tcggccctga cggtcgcggg tgttgccgtt ggcgtggttg gagggctgac ggaagatgct
      781 gatccggccg gtctcctcgt cccagcgcaa gatgcggtta ttgggaatgt cgctccacaa
      841 gagacagcgc gcatcaccga aatagaccgc accctcgtta aaacggcatc cggtcgcaag
      901 ccgctcgact ccggcaagcg cgagccggca tttgttgaac gacggatcga gactctgcac
      961 cgccgggtcg ggataacgct ggctcggctc ccaggccgcc aatgccgcag gcgcgaaggt
     1021 cattgcgcct gctgcacacg cgccagccat catgtcacgg cgtgaatttc ccatggcggc
     1081 ttctcccctg ttttgtgccc gtttacccgg cgctccgtgt cttgcgatgg cacagcatca
     1141 actgcggact catgcaccgc aacatacgac gtgcacaggt attgcaatga cttactcgat
     1201 cacctcgtcg gcgatcgcga gcagcctgtc aggaaccaca ggcccgtttg tcacgatgac
     1261 gtccggcgat tcgcgcatga gctcggccgc atcggccctg acgtgatccg catcggctgc
     1321 gccccagacc acgtcgatcc tggtattgcg gtcctccaac caacccagct cccccaaacg
     1381 ctggcgaagc gctttgacgc gggcgcgcgc atcggggtcg ctcggcccca tgagcaggcc
     1441 gactttccgc atccgctcgg cctgctgcgc gcgcgccgcg agcggccacg ttgttgccgc
     1501 gccgcccagg agagtgatga agtcgcggcg cctcattcaa tcacctcgtc ggcgcgggcg
     1561 agcagcgtcg gcggcatttc gaggccgagc gctttggcgg ttgaggttga tcaccagctc
     1621 gaatttggtc ggcgcctgga ccggcagatc gcccgccttc tcgccgcgca ggatgcgatc
     1681 gacgtacgag gctgcttgcc gatactgatc ggccagatca ttcccgtaag acatcaggcc
     1741 gcccgccgtc acaaattgcc ggtactggta agccgcgggc aaacggtagc gggccgccag
     1801 tgcgaggatc aggtcgcgat gaacgatagt agggccactc ggcagcacga tcagaccgcc
     1861 attcggctca cgggcgaatt ggtcaatagc acgttcgatc tcggcagcat cgcgcacggc
     1921 cgccgccgta agctgcaccc caacggatgg cgccacagct tcaactgcac gcagcatccc
     1981 cgcctgcgga atttgttccg gattgaaaag tacggccacg cgcgtaatct gaggcgccac
     2041 ctctttgagc atctccagtc ttttgccctg cattgagaat tcggcggggg tgaatccagt
     2101 gatgttgcca cccggatgcg ccatgcttgt gacgaagccg ctgccgatcg gatcgcccat
     2161 ctgggtgaag acgatcggta tgctgctcgt cacctgctgc agaggttgca atgcctgcgc
     2221 ggtcacggca aggatgacgt ccggtttcag cccgacaagc tcgatcgcat aggtccttat
     2281 gcgttcgggc tcggcaccgc cccagcgata gtcgatgcgt acgttgcggc ccgcggccca
     2341 acccaatttt tcaagtgcct gccggaacgc cgtcgtatag gcctgcgcat cccgatcgtt
     2401 ctcggcaaa
//
LOCUS       DATLJT010001683         5680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3856203,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001683 DATLJT010000000
VERSION     DATLJT010001683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3856203"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VM910_38350"
     CDS             <1..1427
                     /locus_tag="VM910_38350"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI68396.1"
                     /translation="RTILGVPLLREGTPIGVLSVNRTVVRPFTDKQIDLVQTFADQAV
                     IAIENVRLFDEVQARTRELSESLRQQTATADVLKVISRSAFDLKSVLQTLVESAGRLC
                     GADYATITRQKDGVLFFAEAYGYSSEFIEYVKAMPVERGRGTATGRALLEGRVIHIAD
                     VLADPEYTWAEAQRLGGYRTVLAVPMMREGVPMGVLTLTRSEVRPFSEKQIELVSTFA
                     DQAAIAIENVRLFEEIQDKSKQLAEASEHKSQFLASMSHELRTPLNAIIGLTEMMVTN
                     AARFGTEKALEPLRRVNAAGSHLLSLINEILDLSKIEAGKLDLNLEPVNLSRLIDEVI
                     GTAGQLAEKNKNRLVIEAQENLGRLNADSMRLKQILLNLLSNACKFTKEGEVTLRVRK
                     VADGRDWVELAVADSGIGMTAGQQAKLFQDFTQADSLTARRYGGTGLGLAISRKLARM
                     MGGDVTVTSEPGKGSVFTVRLPAA"
     gene            1597..2574
                     /locus_tag="VM910_38355"
     CDS             1597..2574
                     /locus_tag="VM910_38355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68397.1"
                     /translation="MLTIRMILALTALVTTIAPATAQNWPTRPVTIVYPFAAGSAGDV
                     LGRIFASRLSELLGQPVTFENVGGAGGMTGASRVAKAAPDGYSVLLGTTSTLAVNQTF
                     YKHPLYNAVTDFAPVALIAESPIVLIARRDLPANNLQGFIAYAKANQAKMQYGSAGVG
                     SSVHLACAGLNAAIGVNITHVPYRGGASAMQDLMAGRIDYQCPAAELAIPHIQGNSVK
                     AIAVLTKYRSPTLPNLASAHEQGLANFDAGAWFSFVLPKGTPAAIVQKLHNATVAAMS
                     APATEERLREFGSVLVAPERRSPEYLQKFIQSEIEKWAALIKAANIKAE"
     gene            2776..5019
                     /locus_tag="VM910_38360"
     CDS             2776..5019
                     /locus_tag="VM910_38360"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HVI68398.1"
                     /translation="MSHNENLFKGSIGRTYRESQPWWPDELAAPKGAPNIVIIVLDDV
                     GFADLGCYGSEIATPSMDRLASGGVRYSNFHVTSMCSPTRACLFTGRNAHAVGMGIIA
                     EWSSGYPGYRGHVTAQAATIPEVLRDHAYGTYAVGKWHLTNISHYGAAGPQDNWPLSR
                     GFGRWYGFHGALTDQWNPELYQDNRPIHLHSRETYHLSTDLVDHAIGDIRDHKTSAPH
                     RPFLLYLAFGACHFPHHVPREFIEKYRGRYDIGWDAVRASRLERQHKLGIVPAATGLA
                     PLNPGVTPWHDLAPDVRRLCARLQETYAAFLEHTDVQIGRLIEYLDALGNLEDTLIVL
                     LSDNGASPEGGPTGAFNLRKHMVYEEESPEVGLSRLDDIGGDFAFNHYPTGWAQASNT
                     PLKWYKKDTHGGGIRAPLIIHWPHRISASNAIRAQFHHVVDIAPTLYELVGVEAPTTY
                     RGAPQMPLHGIGMAYTFDADGPTRKEIQHFEMLGDRAIWHRGWKAVARHPKGTDFDAD
                     QWELYHLDDDFAELNDLAAAEPDRLRKMIELWWSEAGKYGVLPLDDRDWERGAERLRM
                     KPNTRYEYLGDMARIDRLSAPDISDRGYVVTASFEADEVAEGVILAWGSRFGGFVLYI
                     KERQLYYEYVYSESQKHTLAVDFRQHPGRRVIQLKFERVGPRAGRASLVVDGAVAGAL
                     DIPRTWPTHGTTAGFNCGRDAGAPVSDAYQRPFRFSGRSLRVTIELEGDVTHNPGAAY
                     QAVLREQ"
     gene            complement(5071..>5680)
                     /locus_tag="VM910_38365"
     CDS             complement(5071..>5680)
                     /locus_tag="VM910_38365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HVI68399.1"
                     /translation="TPGPEGHPEILPIHADANSKRIAGEYWHSDVSCDEEPPLGSILY
                     LHTVPPVGGDTLFASQYAAYEALSSRMKVYLEGLTATHAGDHVYRATNVLVGRDDRGK
                     VFPKASHPVIRTHPVTKRKALFVNQGFTTHIDGLPKEESRAVLNYLFEHCTRPEFQVR
                     FRWRPHSVAFWDNRSVQHMALWDYYPQVRSGRRVTIKGDRPF"
BASE COUNT         1168 a         1671 c         1736 g         1105 t
ORIGIN      
        1 tccgtacaat acttggtgtg ccgctcttgc gggaaggaac tcctatcggc gtgcttagcg
       61 taaaccgcac ggtcgtgcga ccattcaccg acaagcagat cgatttggtc cagaccttcg
      121 ccgaccaggc ggtgatcgcc atcgagaacg tgcggctgtt cgacgaggtg caggcgcgca
      181 cccgggagct gagcgagtcg cttcggcaac aaaccgctac tgccgacgtg ctcaaggtca
      241 tcagccgctc ggcttttgat ctcaagtcag tgctgcaaac gctcgttgaa tcggctggcc
      301 gcctttgcgg cgccgactac gccacgataa ctcggcagaa ggacggagtg ctcttcttcg
      361 cggaggctta cggttactcg tccgagttca tcgaatatgt caaggctatg cctgttgagc
      421 gaggacgcgg gacggcgacc ggacgggcct tgctggaggg tcgggtgatc catattgccg
      481 atgtcttggc cgacccggag tacacctggg cggaggccca gaggttgggc ggctaccgca
      541 cggtcctcgc ggttccgatg atgcgcgaag gcgttccgat gggcgttctg acattgacac
      601 gctccgaggt gcggccgttc agcgaaaagc agatcgaatt agtatcgacc ttcgctgacc
      661 aggcggcgat cgcgatcgag aacgtgcggc tgtttgagga aatccaggac aagagcaagc
      721 agctcgcgga ggcgagcgag cacaagtcgc aattcctcgc cagcatgagc cacgagctgc
      781 gcaccccgct caacgccatc atcggcctga ccgagatgat ggtcaccaac gcggcgcgct
      841 ttggcacaga aaaggcgctg gagccgctgc gccgcgtcaa tgccgccggt agccacctcc
      901 tgagcctgat caatgagata ctcgaccttt cgaagatcga agccggcaag ctcgacctca
      961 atctcgagcc cgtgaatttg tcccggctca tcgatgaggt catcggcacc gcaggacagc
     1021 tcgccgagaa gaacaagaac cggctcgtca tcgaagcgca agagaatttg ggcaggctca
     1081 acgcggattc catgcgcctc aagcagattc tgctcaatct actgagcaat gcctgcaagt
     1141 tcacgaaaga gggcgaggtc acgctgcggg tgcgcaaggt ggcggacgga cgcgactggg
     1201 tcgagcttgc ggtcgcggat agcggcattg gcatgaccgc ggggcagcag gcaaaactgt
     1261 tccaggattt cacgcaggcc gattcactca cggcgcgccg ctacggcggc accgggctgg
     1321 gccttgccat ctcgcgcaag ctcgcgcgca tgatgggcgg cgatgtgacc gtgacaagcg
     1381 agccgggcaa gggttcggtg tttacggtgc gccttccggc tgcctaaaac cttgcggaat
     1441 gcccctgtcg aggtggaagt gccgaaatct ccgctcaggg caagggagca ctatgctgtc
     1501 ggcaggcata ctttttttcg gttgcatcac tcaagacgtc cgtgatgctt cttccgccct
     1561 ggcggcagta atcgcattag tagagtcgga gggatcatgc tgaccatcag aatgattctc
     1621 gccctcacag ccctcgtcac cacgattgcg ccggctacag cgcagaactg gccgacccga
     1681 ccggtgacta ttgtgtatcc atttgctgcg ggcagcgcgg gcgatgtttt ggggcgcatc
     1741 ttcgcatcgc gcctctccga acttttgggc caaccggtga ccttcgaaaa tgttggcggc
     1801 gcgggtggca tgacaggcgc ctcgcgcgtg gcgaaggcag cgcccgacgg ctattcggtt
     1861 ctgctcggca ccaccagcac gcttgccgtg aaccagacgt tctacaaaca tccgctctac
     1921 aatgcggtga ccgactttgc gccggtcgcg ttgatcgccg agagcccgat tgtgttgatc
     1981 gcacgcaggg atttgcccgc gaacaacctg caggggttca tcgcctacgc gaaagcaaac
     2041 caggcgaaga tgcaatatgg ctcggccgga gtgggctcct cggtccatct cgcctgcgcc
     2101 gggctcaatg cagcgatcgg ggttaacatt acgcacgttc cctatcgcgg tggtgcctcg
     2161 gcgatgcagg atttgatggc cggccggatc gactatcagt gtccggccgc tgaactcgcg
     2221 atcccgcaca tccaaggcaa ctcggtcaag gcgatcgcgg tcctaacgaa ataccgctcg
     2281 ccgacccttc cgaatctggc gtccgcgcac gagcaggggc ttgctaattt cgacgctggc
     2341 gcctggtttt cctttgtctt gcccaagggc acgcccgcgg cgatcgtgca gaaacttcac
     2401 aatgccactg ttgctgcgat gagcgctcct gcgaccgagg aacgcctgag ggagtttggc
     2461 tccgttttgg ttgcgcccga acgcaggtcg cccgaatacc tccaaaaatt tatccagagc
     2521 gagatcgaga aatgggcggc cctgatcaag gcggcgaaca tcaaggccga atgattccga
     2581 ccgcatggaa tctagaagac cgctgaggaa ttttgcgatg gtccatatga gcaacattgc
     2641 aacggcaatg cttgaattgc acgattgaaa gtgcgggagg cgggggcacg cgagagcacg
     2701 ccgcgtgcac ttgagcggtc gagcgtctat gagcagcgga ggcaatgtga gctgcctcca
     2761 agagggaagg ccagcatgtc gcacaacgaa aatctcttta agggatcgat cggaaggaca
     2821 tatcgggagt cccagccctg gtggccggat gagctagccg cccccaaagg agcgcccaat
     2881 atcgtaatca ttgttctcga cgacgtaggt tttgccgacc tcggctgcta cggatccgaa
     2941 attgccaccc cgtctatgga ccggctggcg agcggtgggg tgcggtattc caacttccac
     3001 gtcacctcga tgtgctcgcc gacccgcgca tgcctgttca cggggcgcaa cgcgcatgcc
     3061 gttggcatgg ggattatcgc cgaatggtca agcggctatc cgggataccg agggcacgtc
     3121 accgcgcagg cggcgaccat cccagaagtt ctccgcgatc acgcctatgg cacctacgct
     3181 gtcggcaagt ggcatctcac caacatctca cattacggtg cagccgggcc gcaggacaac
     3241 tggccgctaa gtagaggatt tggccgctgg tacggttttc atggtgcgct gaccgaccag
     3301 tggaatccgg agttgtacca ggacaatcgg ccgatccatt tgcattcgcg tgaaacgtat
     3361 cacctcagca cagatctagt ggaccacgcg atcggcgaca ttcgtgacca taagacctcg
     3421 gcgccgcacc ggccgtttct gctctatctc gcgtttggcg cgtgtcactt cccccatcac
     3481 gtgccgcgtg agttcatcga aaaatatcgt ggccgttacg atatcggctg ggatgcggtg
     3541 cgtgcctcac gcttggagag gcaacataag cttggcattg tccccgccgc gaccggatta
     3601 gccccgctca atccgggagt gacgccctgg cacgaccttg cacccgatgt tcggaggctt
     3661 tgcgcgcggc ttcaagagac ctatgccgcc tttctcgagc acaccgatgt gcagatcggc
     3721 aggctgatcg aatatctcga tgcgctcggc aatctagagg acacgctgat cgttctctta
     3781 tcggataatg gtgcaagccc cgaaggaggt cctacgggcg cgttcaattt gcggaagcat
     3841 atggtgtacg aggaggaatc ccccgaggtc ggtctctcgc gtttagacga cattggcggc
     3901 gatttcgcat tcaatcatta cccgactgga tgggcccaag cctccaacac cccgctaaaa
     3961 tggtacaaga aagacaccca cggcggcggc attcgcgcgc cgctgatcat tcactggccg
     4021 caccgaattt ctgcaagcaa tgcaatccgc gcacagtttc accacgtcgt cgacatcgcg
     4081 ccaacgcttt acgagctcgt gggagttgag gcgcccacaa cataccgcgg cgcaccgcaa
     4141 atgccgcttc acggcatcgg catggcctat acgttcgacg ccgatggtcc tacccgcaag
     4201 gagatccagc acttcgaaat gcttggcgat cgtgcgatat ggcatcgcgg ttggaaagcg
     4261 gtggcccgac atccgaaagg aaccgatttt gatgccgacc aatgggagct ttatcacctg
     4321 gatgacgatt tcgcagaatt gaacgatttg gcagcggccg aacccgacag actacgcaag
     4381 atgatcgagt tgtggtggtc cgaagccggc aaatacggtg tcctcccgct cgatgatcgc
     4441 gattgggagc ggggagcgga acgcctcagg atgaagccga acacccgcta cgagtatctc
     4501 ggcgacatgg cgcgaatcga ccgattgtcg gcgcccgata tcagcgatcg cggctacgta
     4561 gtgaccgcct ccttcgaggc ggatgaagtt gcagaagggg tcatacttgc ctggggcagt
     4621 cgctttggcg gttttgtcct ctacatcaaa gaacgacagc tctattacga gtacgtatat
     4681 tcagaatctc aaaagcacac gctggccgtc gattttcgac aacaccctgg caggagggta
     4741 attcagttga agttcgagcg cgtgggccca cgtgctggcc gagcgagcct cgttgtcgac
     4801 ggggccgtcg cgggcgcatt agatattcct cgtacgtggc ctacgcacgg cacgactgca
     4861 ggattcaatt gtggacgcga tgccggtgcg cccgtcagcg atgcctatca gcggccgttt
     4921 cggttcagcg gccgatccct gcgggttacg atcgaacttg aaggcgacgt gacgcataat
     4981 cccggcgctg catatcaggc ggtgctcagg gaacagtgac cgtgcaggat caggttggcg
     5041 caaccgcgac taaatgagcc atacggccgc tcagaacggc ctgtcgccct tgatcgtcac
     5101 gcggcgaccg gagcggacct gggggtaata gtcccacaac gccatgtgct gaacactgcg
     5161 attgtcccaa aatgcgactg agtggggccg ccagcgaaat cgcacctgga attccggtcg
     5221 ggtgcagtgc tcgaatagat aattcaggac ggcgcggctc tcctctttag gaagcccgtc
     5281 gatatgggtg gtaaatccct gattgacgaa cagcgccttg cgcttggtca cgggatgggt
     5341 gcgtatgacc ggatgcgacg ccttcggaaa gaccttgcca cggtcgtcgc gccccaccag
     5401 cacattggtc gcacgataga cgtggtcgcc cgcatgcgta gccgtgagcc cttcgaggta
     5461 gactttcatg cgcgaagaca acgcctcgta ggccgcatac tggctcgcaa acagcgtgtc
     5521 tccgccgaca ggcggcacgg tgtggaggta tagaatgctg cccagcggcg gctcctcgtc
     5581 acatgagacg tcggaatgcc aatattctcc ggcaatgcgc ttggaattgg cgtcggcatg
     5641 aatgggaagg atctcgggat gcccctccgg accgggcgtg
//
LOCUS       DATLJT010001684         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1094863,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001684 DATLJT010000000
VERSION     DATLJT010001684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094863"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..1887)
                     /locus_tag="VM910_38370"
     CDS             complement(475..1887)
                     /locus_tag="VM910_38370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HVI68400.1"
                     /translation="MSKTFRPWKIDEPLLLPVMVQEFVAEDHVACFVLSVVRDEIDLI
                     KITGTYGSERGQPPFNPIMMTALLLYAYCSGIYSSRRIAKACRERVDFMSIVGFDAPD
                     FRTISDFRKRHLNALAELFLQVLHLCAQAGLAKLGHVAFDGSKIKANASKHKAMSYGR
                     MEERAAELEAEVAKWLSTAEAADAEEDQLYGRDKSGEEMPDWVADKQRRVERIRQAKA
                     ELEAEAKAAAEAKLKERAAANEQREAEGRRKGGRPAAPPSTVPDAKAQKNFTDPQSRI
                     MKSKDGFVQAYNAQAAVDAEAQIIVAQDVTQSGSDCAHLLPMIDAVEANLGRRPEQVS
                     ADAGYCSEDNLAGLEDRNIDGYVATGRARDAVAGAAKDNGDPPPSAPQPASTPTRVEV
                     MRAKIKAGGHASPYRLRKQLPEPVFGQIKQARGFRQFLLRGIEKVRAEWALICTAHNL
                     LKLAHRRSLSAALPLAAAAG"
     gene            1972..2925
                     /gene="cysD"
                     /locus_tag="VM910_38375"
     CDS             1972..2925
                     /gene="cysD"
                     /locus_tag="VM910_38375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HVI68401.1"
                     /translation="MNVVSPALEAVPLVAGDPAPPLSHLQALEAESIHIIREAVAESD
                     NPVLLYSIGKDSSVLLHLALKAFHPAKPPFPLLHIDTTWKFRDMIAFRDRRANELGVK
                     LIVHVNREGLARGIGPISHSDLHTDVMKTQGLRQALDHYGFDAALGGARRDEERSRAK
                     ERVFSLRNAQHRWDPKRQRAEPWRLYNGRKRKGESLRVFPLSNWTELDVWLYIHRERI
                     PVVPLYFAAPRPVVCRDGTLIMVDDHRLPLETGEQPQTRTVRFRTLGCYPLTGALESS
                     AMTLAGIIRETAESQFSERRGRLIDHDGSAAMERKKQEGYF"
     gene            2925..>3406
                     /locus_tag="VM910_38380"
     CDS             2925..>3406
                     /locus_tag="VM910_38380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HVI68402.1"
                     /translation="MNVQDPALFDADDFVTRQERKSLLRFIVCGSVDHGKSTLIGRLL
                     FEAGLVCTDQLDALDHESRRFGTQGGERDFALLLDGLAAEREQKITIDIAYRFFATAR
                     RRFIVADAPGHEQYTRNMATGASTADFALLLVSAADGLTRQTRRHARIISTLGVRHIV
                     V"
BASE COUNT          595 a         1136 c         1006 g          669 t
ORIGIN      
        1 gcaaccttgc cggcggtcgg catcctgccg atcggagggc ttacgctgtt gctcggcgtc
       61 gaccgcttca tgtcggagat ccgcgcggcg gcgaatctga ctagcccaac tttaccaatt
      121 cgcagaaaaa agcgaattgg atcaatgcca cctacctaaa agtctccact acagcggcga
      181 gttttctaac gtcgatggac ttttgcgcac atggcaatgc gcggggccat agcctactcc
      241 gcattccgcc gatcgaaaac ccacttggtc tacactacag aggatttgcg ggtacgcttc
      301 gttgatcgga ctcacttttt tgggcgcgcg caccccgaaa aacgtgctta tgcactgcca
      361 attggtaaag ttgggctagg agcctgtcca gattatcgca gttttcagat gtagcgtaag
      421 cttaaggcgg gttggcgggg cgtaacctcg ctttgttacg cccgtttgcc tcctttatcc
      481 tgccgcagcg gccaacggca acgctgcgga cagactccgc ctgtgggcga gcttgagaag
      541 attgtgggcg gtgcagatga gggcccactc ggcgcgtacc ttttcgatgc cgcgcagcag
      601 gaactggcgg aagccgcgcg cctgtttgat ctgcccgaac accggctcgg gcagttgctt
      661 gcgcagccgg tagggactgg catggccgcc ggccttgatc tttgcgcgca tgacctcgac
      721 gcgcgtcggt gtgctggccg gctgcggtgc ggatggcggg ggatcgccgt tgtctttggc
      781 cgcgccggca acggcgtctc tggcccggcc ggtcgccacg tagccgtcga tattgcggtc
      841 ctccagcccc gcgagattgt cctccgagca gtagccggcg tcggctgaca cctgctcggg
      901 tctgcggccg agattagcct cgaccgcatc gatcatcggc agcaggtgag cacaatcgct
      961 cccgctctgc gtcacgtcct gggccacgat gatctgcgct tcggcgtcaa cggcagcctg
     1021 ggcgttgtag gcctgtacga acccgtcctt gctcttcatg atccggctct gcggatcggt
     1081 gaagttcttc tgggctttgg catccggcac cgtggagggc ggcgcggctg gccggccgcc
     1141 tttgcgccgt ccctcggctt cccgctgctc gttcgccgcg gcccgctctt tgagcttggc
     1201 ttcagccgcc gccttcgcct ccgcctccag ttcggctttg gcctgacgaa tccgctcgac
     1261 gcgccgctgc ttgtcggcga cccaatccgg catctcctcg ccgctcttgt cgcggccgta
     1321 cagctggtct tcctccgcgt ccgccgcctc ggccgtgctg agccatttgg caacctcggc
     1381 ttccagttcc gcagcgcgct cctccatgcg cccgtagctc atcgccttgt gcttggaggc
     1441 attggccttg atcttgctgc catcgaatgc cacatgccca agcttcgcca gacccgcctg
     1501 cgcgcacaga tgcaagacct gcaagaacag ttccgcaagc gcattcaaat gccgcttgcg
     1561 gaaatccgag atagtgcgga aatccggcgc atcgaacccc acgatactca tgaaatcgac
     1621 ccgctcgcgg cacgccttgg cgatccggcg cgaggagtag atgccgctgc agtacgcata
     1681 cagcagcagc gccgtcatca tgatgggatt aaacggcggc tggccgcgct cgctgccgta
     1741 ggtgccggtg atcttgatga ggtcgatttc gtccctcacc acactcagca caaagcaggc
     1801 gacatggtcc tcagccacaa actcctgtac catcacaggc aacaatagcg gctcgtcgat
     1861 cttccaaggg cggaatgttt tgctcatgcc cacagacaga atctgactcg cgcttccttg
     1921 accagcaaaa tctgcctatt actcagacag gctcctagag gagacaacgc catgaacgtc
     1981 gtgtcgccgg ccttggaagc cgtaccgttg gttgcaggcg atccggctcc acctctttcc
     2041 catttgcaag cgctcgaagc ggagagcatc cacatcatcc gggaggctgt cgcagagagc
     2101 gacaatccgg tgctgctcta ttcgatcggg aaagactcct cggttctgct gcatctcgcc
     2161 ctcaaggcct tccatccggc gaagccgccg ttcccgctgc tgcacatcga caccacctgg
     2221 aagttccgcg acatgatcgc gttccgggac cgccgcgcga atgagctcgg agtcaagctg
     2281 atcgtccacg tcaatcggga gggcctcgct cgcggcatcg gtccgatcag ccattccgac
     2341 ctccacaccg acgttatgaa gacgcaagga ttgcggcagg cactcgatca ttacgggttt
     2401 gatgccgccc ttggcggcgc ccggcgcgac gaggagcgtt cgcgcgccaa ggaacgcgtg
     2461 ttctcactgc gcaacgcgca acaccgctgg gaccccaaac gccagcgtgc cgagccctgg
     2521 cggctctaca acggccgcaa gcgcaagggc gaaagcctgc gggtgtttcc gctgtccaat
     2581 tggaccgagc tcgatgtgtg gctctacatc catcgcgagc gcatcccggt ggtgcctttg
     2641 tatttcgcag cgccccggcc cgtcgtctgc cgcgacggca cgctgatcat ggtcgacgac
     2701 catcgcctgc cgctcgaaac aggcgagcag ccgcaaaccc gaaccgtgcg gttccgcacg
     2761 ctcggctgct atccgctcac cggcgcgctc gaaagcagcg ccatgaccct cgccggaatc
     2821 atccgggaaa ccgccgagag ccaattctcc gagcggcgtg gccgcctcat cgaccacgat
     2881 ggcagcgccg ccatggaacg caaaaagcag gaagggtatt tctgatgaac gtccaagacc
     2941 ctgcgctgtt cgatgccgac gacttcgtga cgcgtcagga acgcaaaagc ctgttgcggt
     3001 tcatcgtctg cggctccgtc gaccatggca agtcgaccct gattggccgg ctgctctttg
     3061 aggccggcct ggtatgcacc gaccagctcg acgcgctcga tcacgaatcc cgccggttcg
     3121 gcacccaggg cggggagcgc gactttgcgc ttctccttga cggccttgcg gccgaacgcg
     3181 agcagaagat caccatcgac atcgcctacc ggtttttcgc cacggcacgg cgcaggttca
     3241 ttgtcgctga tgcgccgggg cacgagcagt acacccgcaa catggcgacc ggtgcatcga
     3301 cggcagactt tgcgctcctc ctggtcagcg ccgccgacgg attgacccgc cagacccggc
     3361 ggcacgcccg catcatttcg acgctcgggg tgcggcatat cgttgt
//
LOCUS       DATLJT010001685         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3165477,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001685 DATLJT010000000
VERSION     DATLJT010001685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165477"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VM910_38385"
     CDS             complement(<1..233)
                     /locus_tag="VM910_38385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HVI68403.1"
                     /translation="MSREKSESGASKLPKGVIAVSPPSFRRERALIKRGVWPVAGCDE
                     AGRGPLAGPVVAAAVVLDPKRIPKGIDDSKRLTP"
     gene            complement(427..1590)
                     /locus_tag="VM910_38390"
     CDS             complement(427..1590)
                     /locus_tag="VM910_38390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HVI68404.1"
                     /translation="MSRASSHALRHPPATAPSEPAGAPSSFSEIGAAIDRERRRGRGA
                     QSNASGRYEAEARVAFDDGWQSLEELPPFKTTVSLDTARKVITRNDSPDIGFDRSINP
                     YRGCEHGCVYCFARPTHAYLGLSPGLDFESRLYAKPDAPALLEKELAASNYEPRMIAI
                     GTNTDPYQPIERERKIMRGILEVLERAGHPVGIVTKSALVVRDADILSRMAGRNLVKV
                     AISVTTLDAKLARTMEPRAATPTRRLEALKRLCDAGIPTTVMVAPVIPALNDAEIERI
                     LDAAAHAGVKEASYVLLRLPLEVRDLFREWLMANYPDRYRHVFTLIRDMRGGRDYDSQ
                     WGTRMKGTGPMAWMIGRRFEIACEKLGLNKRRSKLTTDHFARPSGNGQQLSLF"
     gene            1764..2330
                     /locus_tag="VM910_38395"
     CDS             1764..2330
                     /locus_tag="VM910_38395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HVI68405.1"
                     /translation="MLSVIIPTEGVEQPAVATLAALVPGAAAGVIREVLLVDSAGNGV
                     IERVADVAGCHFLPFSGPRAAALAAGARQARSPWLMFLRAGAVLDTGWIEETTQFIQS
                     VASSDRPRAGIFRYARSPYADVRLRDRLKSLARLVRPMTDQGLLIARDHYFRLGGHAA
                     DGRRSEAKLLRRLGRSSRTLLRSRIIVL"
     gene            2396..3328
                     /locus_tag="VM910_38400"
     CDS             2396..3328
                     /locus_tag="VM910_38400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HVI68406.1"
                     /translation="MSLGDSDGEIAAVRAFNRAYTKKLGVLDQHLLKSPYSLSEARVM
                     YELAHRDEMTATEIGGELGLDPGYLSRIVQGFEDDGLISREALASDRRHFRLALTTKG
                     RQAFARLDRSSHDEVAAMLSELTVADRKRLTEAMETIERLLGLSRAVPSRPAILRDPR
                     PGDMGYVVQSHGALYAGEYGFDATFEALVAEIVAKFITSFDAARERCWIADIDGDAVG
                     SVFLVRHSDDVAKLRLLLVDPAGRGQGIGQRLVAECISFARACGYRKITLWTQSILLA
                     ARKIYQDAGFKLVASEPHRSFGQDLIGETWEREL"
     gene            complement(3479..4042)
                     /gene="moaB"
                     /locus_tag="VM910_38405"
     CDS             complement(3479..4042)
                     /gene="moaB"
                     /locus_tag="VM910_38405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HVI68407.1"
                     /translation="MSSIDQSKQFIPLNIAVLTVSDTRSLADDKSGTTLAERLTAAGH
                     KLAAREIVSDDIEAIRGVVKKWIADAGVDIIITTGGTGFTGRDVTPEAIEPLFEKRMD
                     GFSIAFHMLSHAKIGTSTIQSRATAGVANATFIFCLPGSPGACRDGWDGILAAQLDYR
                     TRPCNFVEIMPRLDEHLRRPKAKGASA"
     gene            complement(4193..>4315)
                     /locus_tag="VM910_38410"
     CDS             complement(4193..>4315)
                     /locus_tag="VM910_38410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HVI68408.1"
                     /translation="GRVVPDSFTHGSAAQRKQWFTTGVRQGTVEACNTFERGAL"
BASE COUNT          742 a         1384 c         1373 g          816 t
ORIGIN      
        1 ggcgtcagcc gcttcgaatc gtcgatgccc tttggaatcc gcttgggatc gagcacgacg
       61 gcggccgcga cgaccggtcc cgccaatggc ccgcgcccgg cctcgtcaca gccggccacc
      121 ggccagacgc cccgcttgat cagtgcgcgc tcgcggcgaa aactcggcgg cgaaaccgcg
      181 atcacacctt tcggcagctt gctcgcgccg ctttcggact tttcccgact catgccggga
      241 tcctgcttca gggcatgtcg accgcgcaac cgggaacccg gccgaattcc cgcaattcat
      301 ctttctttgc tctgaaatga aaattgacag ccgcagtgga tgggtccgga ccagcatgat
      361 ccggaaaaat gggcaccgat tttcgattac gacaaacgcc ggacgcgttt gcgctgagat
      421 caggctctag aacaggctga gttgctggcc gttgccgctc ggccttgcga agtgatcggt
      481 ggtgagcttt gagcggcgct tgttcagccc aagcttttcg catgcgattt cgaacctgcg
      541 cccgatcatc caggccatcg gcccagttcc cttcatccgc gtaccccatt gcgagtcgta
      601 gtcgcggccg ccgcgcatgt ctcggatcag cgtgaagaca tggcgatagc gatcggggta
      661 attggccatc agccattcgc ggaaaagatc gcgcacctcg agcggcaggc gcaacagcac
      721 gtagctcgcc tccttgacgc cggcatgggc cgccgcgtcg agaatacgct cgatctcggc
      781 gtcgttcagg gccgggatca ccggcgccac catgaccgtg gtgggaatgc ctgcgtcgca
      841 caaccgcttg agcgcctcca ggcgccgcgt cggtgtcgcc gcccgcggtt ccatggttcg
      901 cgcgagtttc gcatcgagcg tcgtcaccga aatcgccacc ttgacgaggt tgcgcccggc
      961 catgcgcgac agaatatcgg cgtcgcggac caccagtgcc gacttggtga cgatgccgac
     1021 cggatggccg gctcgctcca gcacttcgag aatgccgcgc atgatcttcc gctcgcgttc
     1081 gatcggttga tacggatcgg tattggttcc gatcgcgatc atccgcggct cgtagttgga
     1141 cgccgccagt tccttctcga gaagcgccgg cgcatccggc ttggcataaa ggcgggattc
     1201 gaaatcgaga cccggggaaa ggccgagata ggcatgcgtt ggccgcgcaa agcagtagac
     1261 gcaaccatgc tcgcagcccc gataagggtt gatcgagcgg tcgaagccga tatcggggga
     1321 atcgttgcgt gtaatgacct ttcgcgcggt gtcgagcgac accgtggtct tgaacggggg
     1381 caattcttcc aggctctgcc agccgtcgtc gaaggcgacc cgcgcttccg cctcatagcg
     1441 cccgctcgca ttggactgtg cgccgcgtcc ccgccgccgc tcgcggtcga tagcggcccc
     1501 gatctcggaa aaagatgaag gcgcacccgc cggttcggag ggcgccgtgg ccggcgggtg
     1561 cctgagggca tgagaggatg ctcgactcat gggttcacct tagccatgcc tatagaacaa
     1621 agcaagaaca atgctgagga accggccggc ttggatcgta gttgcctgcc tgaaaaaggc
     1681 gcatcggact ggctgttttg attgtgacaa ggtgataaca gtcggcgttt ttcccaatct
     1741 cgtgagccaa cgaagcctca tcgatgctga gcgtcatcat tcccaccgaa ggcgtcgaac
     1801 agccggcagt cgcaaccctt gccgcgctgg tgcctggagc agccgccggt gtcatccgcg
     1861 aagtgctgct ggtcgatagt gcaggcaacg gcgtgatcga acgcgtcgcc gacgttgcgg
     1921 gctgccactt tctccccttt tcgggaccgc gcgccgcggc gcttgcggcc ggcgcccggc
     1981 aggcgcgctc gccctggctg atgttcctgc gcgccggcgc cgtgctggac accggctgga
     2041 tcgaggaaac cacgcagttc atccagagcg ttgcatcgag cgaccgtcct cgcgccggca
     2101 ttttccgcta cgcccgctcg ccctatgccg acgtgcgctt gcgtgatcgc ctcaaatcgc
     2161 tcgcgcgact ggtccggccg atgacggacc agggacttct gatcgcgcgc gaccactatt
     2221 tccggctcgg cggccacgcg gccgatgggc gccgctcgga ggccaaattg cttcgccggc
     2281 tcggccgctc atcgcgcacg cttctgcgca gccggatcat cgtcctgtga tggacgtgga
     2341 agatacttgc caaagtcaag ttattatttg acaatggcaa gtatcgaggt gcgccatgtc
     2401 gttaggcgat tccgatggcg agatagccgc cgtccgcgcg ttcaaccgcg cctataccaa
     2461 gaagctcgga gtgctggacc agcatctcct gaaaagccct tattcgttgt ccgaggcgcg
     2521 cgtgatgtac gagcttgcgc accgcgacga aatgacggca acggagatcg gcggcgagct
     2581 tggcctcgat ccaggctatc tcagccgcat cgtccagggt tttgaggatg acgggctgat
     2641 cagccgtgag gcccttgctt ccgaccgcag acacttccgc ctcgcactga ccacgaaggg
     2701 acgccaggcg tttgcccggc tcgatcgcag ctcccatgac gaggtcgcgg cgatgctgtc
     2761 ggaacttacc gtcgccgacc gcaaacgcct gacggaggcg atggagacca tcgagcggct
     2821 gcttggtctg tcgcgcgcgg ttccgtcccg cccggcgatc ctgcgcgatc cgcgccccgg
     2881 cgacatgggt tatgtggtgc aaagccatgg cgcgctctat gccggcgaat acggtttcga
     2941 cgcgaccttc gaggcgctgg tggcggagat cgtcgcgaaa ttcatcacct cgttcgacgc
     3001 cgcgcgcgaa cgctgctgga tcgccgatat cgacggcgat gcggtcggct cggtgtttct
     3061 ggtgcggcac agcgacgacg tcgcgaaact gcgcctgctg ctggtcgatc ccgccggccg
     3121 cggacagggc atcggccagc gactggtcgc cgaatgcatc agcttcgccc gcgcctgcgg
     3181 ctaccgcaag atcacgctat ggacccagag tattttgctc gccgcccgca agatctacca
     3241 ggacgccggt ttcaagctcg tggcgagcga accgcaccgc agctttggcc aggacctgat
     3301 cggggagacc tgggaacgcg agctgtgatc ttcgcggctc tattgcgcgc gaccgcggcc
     3361 aaggcgatac gcgggttttt cggattcgag gtctgcgtgt agcgggtttc tgaattgctt
     3421 cgctcgcact cgcaatgatc ttggattgct gcgtcgctct tctccgcgcg atgacaactt
     3481 aagccgaagc ccccttcgcc ttcggccgcc gcaggtgctc gtcgagccgc ggcatgatct
     3541 ccacgaagtt gcagggccgc gtgcggtaat cgagctgcgc agccagaatg ccgtcccaac
     3601 cgtcgcggca ggcgccgggc gagccgggca ggcaaaagat gaaggtcgcg ttcgcgacgc
     3661 cggcggtggc gcggctctgg atcgtcgagg tgccgatctt ggcgtggctc agcatgtgaa
     3721 acgcgatcga aaagccgtcc atccgttttt cgaacagggg ctcgatcgct tccggcgtga
     3781 cgtcgcgccc cgtaaagccg gtgccaccgg tggtgatgat gatatcaacg cctgcatccg
     3841 cgatccattt tttgacgacg ccacggatcg cctcgatgtc gtcggacaca atctcgcgcg
     3901 cggcgagctt gtgtccggca gcggtgagcc gctcggcgag cgtggtgccg gacttgtcgt
     3961 cggcgagcga gcgcgtgtcc gagaccgtca gcacggcgat gttgagcggg atgaattgtt
     4021 tggactgatc gatggaggac attcacttca ccctcgtgtc ccgggcacgg gtgcagcgtg
     4081 acagcgatgc accgcagagc cgacacccat tcaaaacacc atatccgatg gtgggtcccc
     4141 gatcagcagc gcagcatttc gtgccgcgct gcatccgggg cacgctccgt cgctacagcg
     4201 cgcccctttc gaacgtgttg caggcttcca ccgtgccctg ccgaacgccg gtcgtgaacc
     4261 attgcttgcg ctgggctgcg gagccgtggg taaaggaatc cggcaccacg cgtcc
//
LOCUS       DATLJT010001686         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2245861,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001686 DATLJT010000000
VERSION     DATLJT010001686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245861"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1287)
                     /locus_tag="VM910_38415"
     CDS             complement(232..1287)
                     /locus_tag="VM910_38415"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HVI68409.1"
                     /translation="MAAKNAIPTIDLSPLRGGSGTEKREVARQIDAACTEIGFFLVTG
                     HGISQDLITTARQRAIDFFALPDEEKMKVQRPPAKISRGYNWVGDRSVAYSMGQAAPP
                     DIQEAFAFGPESVADLASRVDAASAKMYAPNIWPDRPADFKKIMLSYYGAMSDLASDV
                     LRAMATSLGVSEAYFTDKFDRQASVARVIRYPAVTQPPLPGQLRAGTHTDYGIMTFVR
                     GDDTPGGLQVKHRQGGWIDVHIPSDGFCCNIGDLMARWSHDRWVSTLHRVAVPPPDAV
                     PTDRISLVFFQNPNPDTVIRCFESCVRPGETEKYPPITVAEHYLGKLMKAGHSRLDAK
                     VEDALTDAKRRESQATQ"
     gene            1444..>2084
                     /locus_tag="VM910_38420"
     CDS             1444..>2084
                     /locus_tag="VM910_38420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68410.1"
                     /translation="MLAPGIVRAQAIFPNHPIRIVVPVSPGGGVDTFARLIAAKVKTQ
                     RNVQFFVENRTGGNSTIGGLDVRRAAPDGYTVLFHASTHNVARLVMRNVPYDPVADFT
                     PIALAGSALLVHIVSNSRQEKTLAELVAAGKANPDKWSFATAQLGAPGHLAAVAFNQY
                     NGLNLPIIVYRGTAPAANDVVGGHVPMMIEAILALLPMVRAGSVRAIAVTSTKR"
BASE COUNT          378 a          659 c          639 g          408 t
ORIGIN      
        1 gattgctgcg ttgcgggatt gcgatccggt ctatgtccgc tttgggtcat aagcggaaat
       61 actcgatctc agcattagat gtccgcttta cccccaaaag cggacatccg atctgcgcgt
      121 tttaatgagt acacgcccta gatcacgcgc gtcgaccgga atgtcgccgg ccgggggtcc
      181 tcgaggatct tctccatgaa cactgacgct cgaaggcagg ctgcgagggg attattgggt
      241 agcttgggac tcgcgccgtt tggcatccgt caatgcatcc tcgaccttgg catcaagccg
      301 gctgtggcct gccttcatca atttgccgag gtagtgctcc gcaacggtga tgggcggata
      361 tttctctgtc tcgcccggtc tcacgcaact ctcgaagcag cgaatgaccg tgtcgggatt
      421 tgggttctga aagaagacaa gcgagatgcg atccgtcggc acggcgtctg gcggtggcac
      481 cgccacgcga tgcagggtcg atacccatcg gtcgtgcgac cagcgcgcca ttagatcgcc
      541 aatgttgcag caaaacccgt cactcggaat atgcacatcg atccagccgc cttggcgatg
      601 cttgacctgc agcccgcccg gcgtatcgtc accgcgcacg aacgtcatga ttccgtagtc
      661 cgtgtgggtg ccggcgcgaa gctgtcccgg caacggcggc tgggtcaccg ccggataacg
      721 gattactcgg gccacgctcg cttggcgatc gaacttatcg gtgaaatagg cctcgctcac
      781 gccaagcgac gtcgccatgg cgcgcagcac atccgacgcc aagtcagaca tggcgccgta
      841 gtaggaaagc atgatcttct tgaagtcggc cggacggtcc ggccagatgt tcggcgcgta
      901 cattttcgcg ctggcggcgt cgacccgcga tgccagatcg gcgacgcttt cgggcccgaa
      961 tgcaaaggcc tcttgaatat cgggcggcgc cgcctggccc atcgaatatg caaccgaccg
     1021 gtcgccgacc caattgtagc cgcggctgat cttggcgggg ggccgctgca ccttcatctt
     1081 ctcttcgtcg ggcagcgcga agaagtcgat cgcccgctgg cgcgccgtcg tgatcagatc
     1141 ctgagagatg ccatgcccgg tgactaggaa aaatccgatc tcggtgcagg ccgcgtcgat
     1201 ctggcgcgcc acctcgcgct tctcggtccc gctgccgccg cggagcggcg acaggtcgat
     1261 tgtagggatc gcattcttcg ccgccatggt gcatccttcc accgtgtgct tggcaactgc
     1321 gctcgaccat agtcgctgaa ttgatgccgc tcgcgatagt ataagagcga acggcatcga
     1381 gtaaggacca tttctacgaa tcgtcgtgac gcaatttcat cgctcctggc gactgcgggc
     1441 gccgtgctcg cgccgggtat cgtgcgcgcg caagcgattt tccccaatca cccaatccgc
     1501 atcgtcgttc ccgtatcgcc gggtggaggc gtcgacacct tcgcgcgcct cattgcagcc
     1561 aaggtcaaga cccagcgcaa cgttcagttc ttcgttgaga accgtaccgg cggcaatagc
     1621 acgatcgggg gcctcgacgt ccggcgcgcc gcgccggatg gctacacggt gctgttccac
     1681 gcctcgacgc acaatgtcgc gcggctggta atgcgcaatg tgccctacga tcccgtcgcg
     1741 gatttcacgc cgatcgcgct cgccggcagc gcgctgctgg tgcacatcgt ttcgaacagc
     1801 cggcaggaga agacgctggc ggaactcgtc gccgcaggca aggcaaaccc cgacaagtgg
     1861 tccttcgcga cggcgcagct cggcgcgccc ggtcatctcg ccgccgttgc gttcaatcaa
     1921 tacaacggcc tcaatctgcc gatcatcgtt tatcgcggga cagcaccggc cgcgaacgat
     1981 gtcgtcggcg ggcacgtgcc gatgatgatc gaggcgattc ttgccctgct gccgatggtg
     2041 cgcgctggaa gcgtgcgggc aatcgccgtg acaagcacga agcg
//
LOCUS       DATLJT010001687         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_177623,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001687 DATLJT010000000
VERSION     DATLJT010001687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177623"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM910_38425"
     CDS             complement(<1..206)
                     /locus_tag="VM910_38425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68411.1"
                     /translation="MPAQSTITAIDTLLEPDATMLGHAQANNTRLLKVFPKGFALDEA
                     HRPHITLIQRFVRTEDLDQYYAAA"
     gene            complement(239..1165)
                     /locus_tag="VM910_38430"
     CDS             complement(239..1165)
                     /locus_tag="VM910_38430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVI68412.1"
                     /translation="MAATGKPNALPTWNDGAAKSQILDFVARVTEQGGPDFVAPTERV
                     ATFDNDGTLWCEQPLQTQAFFLIDRTKELAAKDPTLKERQPFKALLEQDLKTLMGLGR
                     QAILELVFATHSGMREEEFEPIARDWLAKARHPKFGRLFKECTYRPQFELLNFLRQSG
                     FKTYIVTGGGIDFVRACSEEIYGIPREQVIGSSQKLRFEVNEDQVGVMKLAELNSFDD
                     REAKPQNIALHIGRRPILAFGNSDGDLPMLRYTKTGKSPRMALLLHHDDAEREVAYDR
                     QFRLSPLNEGLEKADAYGITVVSMKRDWNVIF"
     gene            1385..1651
                     /locus_tag="VM910_38435"
                     /pseudo
     CDS             1385..1651
                     /locus_tag="VM910_38435"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            1690..>2192
                     /locus_tag="VM910_38440"
     CDS             1690..>2192
                     /locus_tag="VM910_38440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68413.1"
                     /translation="MVRVRGRGNPATGSPAAVGVSLLALAGCGPAAPDPSVAERFAAT
                     AAVQQRDALTVRVRALSAEESRTYFGASPARVGVQPVWVQVQNDSDSDARYLPILTDP
                     NYFAPQEVAEQLHGWLSAVRNARLDALFARSAMPIYVAPHSVAAGFIYTHPDGGLKLI
                     NVGLIAG"
BASE COUNT          391 a          677 c          666 g          458 t
ORIGIN      
        1 tcggcagccg catagtattg gtcgaggtcc tccgtgcgga cgaaccgctg tatcagcgtg
       61 atgtgcggac ggtgtgcctc atccagggcg aatcccttcg ggaagacttt gagcaatcga
      121 gtgttgttgg cttgtgcgtg acccagcatg gtggcgtcag gttccagcag cgtgtcgatg
      181 gccgtgatgg tggattgtgc aggcatgacg acctccactt aggttctagg agcacgtttt
      241 agaaaatgac gttccagtcg cgcttcatgc taaccaccgt gatcccgtac gcgtccgctt
      301 tctccagccc ctcgttaagc ggactgagcc ggaactggcg gtcataggcg acttctcgct
      361 cagcgtcgtc atggtgaagg agaagagcca tgcgcgggct tttgccggtt ttggtatacc
      421 gcagcatcgg cagatcacca tccgagttgc caaacgccag aatcggccgc cgaccgatgt
      481 gcagagcgat gttctgcggc ttcgcctcgc gatcgtcgaa gctattgagt tcggcaagct
      541 tcatcacccc aacttggtcc tcgttcactt caaaccttag cttctggctc gagccgatta
      601 cctgctcgcg tgggatgcca taaatttcct ccgagcacgc gcgcacgaaa tcgatgccac
      661 cgcccgtcac gatgtaggtc ttgaacccgc tctgccgcag gaaattgagc agctcgaact
      721 gcggccggta cgtgcattcc ttgaacagcc gcccgaactt gggatgcctc gccttcgcca
      781 gccagtcgcg cgcgatgggt tcgaactcct cctccctcat accggaatgc gtggcaaata
      841 ccagctccaa gatcgcctgc ctccccagcc ccatcagcgt cttgaggtcc tgctcgagca
      901 acgctttgaa cggctggcgt tccttcagcg tagggtcctt cgcggcgagc tcttttgtgc
      961 gatcgatgag gaaaaaggct tgtgtctgca gcggctgctc gcaccaaagc gtgccatcgt
     1021 tgtcgaaggt ggcgacgcgc tctgtcgggg cgacgaaatc gggtccacct tgctctgtca
     1081 cgcgcgcgac aaagtcgagg atctgggatt tagctgcacc gtcgttccag gtggggagtg
     1141 cattcggctt accggttgcc gccatgggtc acccttgctg gtgatgggtc atgtggatat
     1201 tgacgccgcc cttctcgagc tgctcgcgaa cccactcgac tcgattccat tcagaaaacc
     1261 gagcttagag gctctttgct tggtgcgcat atccgaccat agtctcattc aacccctggt
     1321 ccagtaacca agtgagagtc cgaacgccaa ggccaagcgc acgtttccgc cctggcccac
     1381 cgacgtcggt gacggtgctc agctggacga aggctcaccg gcgcgcgacg ccgacaagat
     1441 caaacgtccc gtgctgctac ttcacggcgc gctcgatcgc gatgttgtga tagcggagtc
     1501 gcgacatatg gcgcagagtc ccgaggaggc gggcgtacca tacgaactcg taacgtggga
     1561 cgatcttgat catcaactcg atggcgcggc cgcgcgcgcg gaaatgctgc gcaacagcga
     1621 gacatttctg ctgcgatcgg tcggcgagta agcgttgcgg cgggcctcac tttgaaccga
     1681 ggtgacccga tggtgcgagt tcgcggtcgc ggcaatccag ccaccgggtc gcctgctgca
     1741 gttggcgtct cgctgctcgc gctggcgggt tgtgggcccg cggcgccgga tccgagcgtt
     1801 gccgagcggt ttgccgccac cgccgcggtg cagcaacgcg atgcgctcac cgtacgcgtg
     1861 cgagccttgt ccgcagaaga aagccgcacc tacttcggtg cctcgcccgc tcgcgtgggg
     1921 gtgcaaccgg tctgggtaca ggtgcagaac gacagcgata gcgacgctcg ttacctgccg
     1981 atcctaaccg atcccaacta cttcgcgccg caggaggttg ccgagcagct gcacggctgg
     2041 ctctccgcgg tgcgtaatgc gcgcctggac gcgctctttg cgcgcagcgc catgcctatc
     2101 tatgtggcgc cgcactcggt ggcagccgga ttcatctata cgcaccccga cggtggtctc
     2161 aaattgatca acgtcgggtt gattgccggg ca
//
LOCUS       DATLJT010001688         3305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1095733,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001688 DATLJT010000000
VERSION     DATLJT010001688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095733"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VM910_38445"
     CDS             complement(<1..242)
                     /locus_tag="VM910_38445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HVI68414.1"
                     /translation="MNDIPGVLLAGGLARRIGGGDKPMRTIGGRTILERVIARLSPQC
                     DGLILNANGDPARFAAFGLPVVADGVAGFPGPLAGIL"
     gene            complement(235..1125)
                     /locus_tag="VM910_38450"
     CDS             complement(235..1125)
                     /locus_tag="VM910_38450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HVI68415.1"
                     /translation="MMKIEKPPVPLVVPNPDDPRLTERVTGIDQTGRPTEIKVPVERA
                     LTLYLNAQEIVTMMTIGDYPEYLALGYLLNQNMLKYDDLVTEVEYDDDLQVVVVRTAH
                     HTNFEQKLRKRTQTSGCAQGTAFGDLMEAVESVVLPKAELRTSWLYQMTHAINTMPSL
                     YLEAGAIHGCVLCKEGTPVCYTEDVGRHNAVDKIAGWMFRHGVDPADKILYTTGRLTS
                     EMVIKTVRMGIPILVSRSGFTAWGVDLARQVGLTLVGRTRGKRFIALSGEERIVHDQN
                     LDYVEEESARHRRKGEARDE"
     gene            1221..1748
                     /gene="mobB"
                     /locus_tag="VM910_38455"
     CDS             1221..1748
                     /gene="mobB"
                     /locus_tag="VM910_38455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HVI68416.1"
                     /translation="MKVIGLAGWSGAGKTTLLTRVIPHLRAAGLRVSVIKHAHHKFDV
                     DVPGKDSWRHRQAGAEEVLVSSDMRWALMHELRGAREPRLPELLAKLSRVDLVIVEGY
                     KSEPHRKIEVHRAANGKPFLYPDDPAIAGIASDVAVETVLPTARLDDIPAVAAMMRRL
                     AISIEDMPAMADADS"
     gene            1760..3013
                     /gene="glp"
                     /locus_tag="VM910_38460"
     CDS             1760..3013
                     /gene="glp"
                     /locus_tag="VM910_38460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVI68417.1"
                     /translation="MAQLSDDCFAFGGPMMSVDEAVGIITSRVAAVKDRETVALGEAD
                     GRVLACDVAAPLPLPPFTNSAVDGYAVQSRDLPQAKERAFPVTGRVAAGASAKAAQPG
                     QAVRIFTGAPMPDGTDTVFMQEDVRIDEAGHVVLPAGLKPGANVRPAGEDVPQGRVAL
                     KAGQRLRPQDVALAAAFGLTQIEVVRRLRVAVFSTGDELASPGAPRAAAQLFDSNRFM
                     LMAMLKRLGCEVSDLGILRDERAPLANALKQVAARHDLILTTGGVSTGEEDHVKAGVE
                     SAGTLVLWRMAIKPGRPVAMGIIGGTPFIGLPGNPVASFVTFVHVVRPTVLALSGAIA
                     TPPDPMPVRSAFSYRKKSGRREYVRVNLRKAPDGALEAVKFPREGAGLLSSLVDTDGL
                     VELGETVTGVEPGETVGFLGYASLL"
     gene            3142..>3305
                     /locus_tag="VM910_38465"
     CDS             3142..>3305
                     /locus_tag="VM910_38465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HVI68418.1"
                     /translation="MTTTKLDLTGLKCPLPALKTRKALKSLKPGDRLEVHCTDPLSVI
                     DIPNLIQETG"
BASE COUNT          573 a         1049 c         1085 g          598 t
ORIGIN      
        1 aggatgccgg cgagcggacc cggaaaaccg gcgacgccgt cggcgacgac cggcaaacca
       61 aaggcggcaa agcgcgccgg atcgccgttg gcgttgagga tcagcccgtc gcattgcggc
      121 gaaagacgcg cgatcacccg ctcgaggatg gtgcggccgc cgatcgtgcg catcggcttg
      181 tcgccgccgc ctattctgcg cgccagacca ccggcgagca gcacgcccgg aatatcattc
      241 atcgcgcgct tcgcccttgc gccggtgccg tgcggattct tcttcgacgt agtcgaggtt
      301 ctggtcgtga acgatgcgct cttctcccga cagggcgatg aagcgctttc cccgcgtgcg
      361 cccgaccagc gtcagcccga cctgtcgcgc cagatcgacg ccccaggcgg tgaaacccga
      421 gcgcgagacg aggatcggaa tccccatccg caccgtcttg atcaccatct ccgaggtcag
      481 acggcccgtg gtgtagagaa tcttgtcggc gggatcgacg ccgtggcgga acatccagcc
      541 cgcgatcttg tcgacggcgt tatggcgtcc gacgtcctcg gtgtaacaga cgggcgtccc
      601 ttccttgcac agcacgcagc cgtggatcgc gccggcctcg agatagagcg agggcatggt
      661 gttgatcgca tgcgtcatct gatagagcca tgaggtgcgc agctccgcct tcggcagcac
      721 cacgctctcg accgcttcca tcagatcgcc gaacgccgtg ccctgcgcgc agccggaggt
      781 ctgcgtgcgc ttccttaatt tttgctcgaa atttgtgtga tgggcggtgc gcactacgac
      841 cacctgcaaa tcgtcgtcgt actcgacctc ggtgacgagg tcgtcgtatt tcaacatgtt
      901 ctggtttagc aggtagccga gcgcgagata ttccggatag tcgccgatcg tcatcatggt
      961 gacgatctcc tgcgcgttga gataaagcgt gagcgcccgc tccaccggca ccttgatctc
     1021 ggtcgggcgg ccggtctggt cgatcccggt cacgcgctcg gtcaggcgcg gatcgtccgg
     1081 attcgggacg accaggggaa cggggggctt ttcgatcttc atcataaggc taggttagca
     1141 tgacaagggt ccaagaccga tataaagcac gtttaggcat tgcctagccc ctcggttccg
     1201 ggcgaggctc ggagaatacg atgaaagtga tcgggcttgc gggatggagc ggcgcgggga
     1261 agaccaccct tctgacgcgc gtcatcccgc atttacgggc ggcgggcctg cgggtctccg
     1321 tcatcaagca cgcccaccac aagttcgatg tcgacgttcc cggcaaggac tcctggcgcc
     1381 accgtcaggc cggcgccgaa gaagtgctgg tctcttccga catgcgctgg gcgctgatgc
     1441 atgagctgcg cggcgcgcgc gagccgcgtc ttccggagct gcttgcaaag ctctcgcgtg
     1501 tcgatctcgt catcgtcgag ggctacaaat ccgagccgca ccgcaagatc gaggtgcatc
     1561 gcgcagccaa cggcaagcct ttcctttacc ctgatgatcc cgcgatcgcc ggtatcgcca
     1621 gcgatgtcgc agtcgaaacc gtgcttccca ccgcccgtct cgacgatatc ccggctgtcg
     1681 ccgccatgat gcggcggctg gcgatctcga tcgaggatat gccggcgatg gccgacgccg
     1741 acagctaaag gcgcgcgaca tggcgcagct atccgatgat tgctttgcct tcggcggacc
     1801 gatgatgtcg gtcgatgagg ccgtcggcat catcacctcg cgcgttgcgg cggtaaagga
     1861 cagagaaacc gtcgcgcttg gcgaggccga cggccgcgtg ctggcgtgcg atgttgcggc
     1921 cccgttgccg ttgccgccgt tcaccaattc cgcggtggac ggctatgccg tgcaatcacg
     1981 cgatttgccg caagcaaagg agcgggcctt tccagtgacg ggacgggtgg ctgcgggcgc
     2041 atcggcgaaa gccgcgcagc caggccaagc ggtacgaatt ttcaccggcg cgccgatgcc
     2101 tgatggcacc gacaccgtgt tcatgcagga agacgtgcgg atcgacgagg cgggccatgt
     2161 cgttctcccg gccggcctca agccgggcgc caatgtgcgc cccgccggcg aagacgttcc
     2221 gcaaggccgt gtcgcgctca aagcgggaca acgcctgcgg ccgcaggacg tcgcgctcgc
     2281 cgcggcgttc ggcctgacgc agatcgaggt ggtcaggcgg ctgcgcgttg ccgtgttttc
     2341 gaccggcgat gaactggcct cgcccggcgc gccacgcgcc gcagcccagc tcttcgattc
     2401 caaccggttc atgctgatgg cgatgctgaa gcggctcggt tgcgaggtct ccgatctcgg
     2461 cattctgcgc gacgagcgcg cgccgcttgc gaacgctttg aagcaggtgg cggcacgcca
     2521 cgatctgatc ctcaccacgg gcggcgtctc gaccggcgag gaggaccacg tcaaggccgg
     2581 tgtcgaaagc gctggcacgc tggtgttatg gcggatggcg atcaagcctg gtcggcccgt
     2641 cgcgatgggg atcattggcg gcacgccgtt catcggcctg ccgggaaatc cggtggcgag
     2701 ctttgtgacc ttcgtccatg tggtgcggcc gacagtgctg gcattgtcgg gggcgatcgc
     2761 aacgccgccc gatccgatgc cggtgcgatc cgccttcagc tacagaaaga agagcggccg
     2821 ccgcgaatat gtccgcgtca acctgcgcaa agcgcctgac ggcgcgctgg aagcagtgaa
     2881 attcccgcgc gagggcgcag gcttgttgtc atcgctggtc gataccgatg ggctggtcga
     2941 gctcggcgaa accgtcaccg gggtcgagcc gggcgagacc gtcggcttcc ttggctacgc
     3001 aagcctgcta tagcgcatga tccggaaaag tggcgactgg ctttccctcg cgacaaacgc
     3061 agaacgcgtt tgcgcggaga tcatgcgcaa acaatgtgtg acgccgttga ccttgccgcc
     3121 cggtttttcc atgttgcgcg catgaccacg accaagctcg atctgaccgg gctcaaatgc
     3181 ccgctgccgg cgctcaagac gcgcaaggcg ctgaaatcct tgaagccggg tgacaggctg
     3241 gaggtgcatt gcaccgatcc attgtcggtg atcgacattc cgaacctgat ccaggagacc
     3301 ggcga
//
LOCUS       DATLJT010001689         1541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1096051,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001689 DATLJT010000000
VERSION     DATLJT010001689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096051"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..970
                     /locus_tag="VM910_38470"
     CDS             233..970
                     /locus_tag="VM910_38470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HVI68419.1"
                     /translation="MNKVYADARSALAGLLKDGMMIMAGGFGLCGIPETLILAIRDSG
                     VKNLTVVSNNCGVDGKGLGILLDTRQIKKMISSYVGENKTFMQQYLAGELEIEFNPQG
                     TLAERIRAGGAGIPAFFTRTGVGTQIAEGKEVREFDGHRYVMERGLVADLAIVHAYRG
                     DSEGNLVYRKTARNFNPPMATAAKVTVVEVEQLVQPGDINPDHIVTPGIYVQRIVHVP
                     NAVKHIEQRTVRKRQRADSPAGTGVEV"
     gene            1020..>1541
                     /locus_tag="VM910_38475"
     CDS             1020..>1541
                     /locus_tag="VM910_38475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HVI68420.1"
                     /translation="MAWTREQMAARAAKELQDGFYVNLGIGIPTLVSNYIPPGMRVQL
                     QSENGMLGTGPFPFEGEEDPDLINAGKQTVTELPTTSYFNSSDSFAMIRGGHIDIAIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVGGVKKVVVVMEHSAKDESKLKHRCTLP
                     LTGAGVVDMIITDL"
BASE COUNT          331 a          445 c          462 g          303 t
ORIGIN      
        1 ttttcgcctt cgtagcgtcc ttgctgggcg gccttatttc atttcgcaac cggatcggcg
       61 gagcgctgct cgcggcaatc ttgttcgctg cgatgtatgt ggtttggaac tactatccgc
      121 atccgcaaat tcagcttccg atcgtgagcg cgtattccac gccatccggc ggttgacctt
      181 tccgtccatc cgattatcgc tattccgggg cattactgaa aatggagcgg acatgaacaa
      241 ggtctatgcg gacgcgcggt ccgcgttggc cgggttgctg aaggacggga tgatgatcat
      301 ggccggcggc ttcggcctat gcggcattcc cgaaacactg atactggcaa tccgcgactc
      361 tggggtgaaa aacctcaccg tcgtctccaa caattgcggc gttgacggca aagggctcgg
      421 cattctgctc gacacccgcc agatcaagaa gatgatctcc tcctatgtcg gggagaataa
      481 aactttcatg cagcaatatt tggctggcga actcgaaatc gagttcaacc cgcaaggcac
      541 gctggccgag cgcattcgcg ctggcggcgc cggcattcca gcattcttca cccgcaccgg
      601 tgtcggcacg cagattgcgg agggcaagga ggtgcgcgag ttcgacggcc accgttacgt
      661 catggagcgc ggcctggtcg ccgacctcgc catcgtgcac gcctatcgcg gcgattccga
      721 gggcaatctc gtctaccgaa agacggcgcg caacttcaat ccgccgatgg caacggcggc
      781 caaggtgacg gtggtcgaag tcgagcagct ggtgcagccc ggcgatatca atcccgatca
      841 catcgtcacg cctgggattt atgtgcaacg cattgtgcat gtgccgaacg cggtcaagca
      901 catcgagcag cgaaccgtga gaaaacgaca gagagccgac tcgccggccg gaaccggcgt
      961 cgaagtataa acacgcatga tcccgaaaag tgggaaggta aaaaaggagc cgaacgacaa
     1021 tggcttggac ccgtgagcag atggcggcgc gcgccgccaa ggaactgcaa gacggcttct
     1081 atgtgaacct cggcatcgga attccgacac tggtgtccaa ctacattcct cccggcatga
     1141 gggtgcagct gcaatcggag aatgggatgc tcggcactgg tccgttcccg ttcgagggtg
     1201 aggaggatcc cgacctcatt aatgccggca agcagacggt taccgagttg ccgaccacga
     1261 gctatttcaa ttcgtcggat tccttcgcca tgattcgcgg cggtcacatc gacatcgcca
     1321 ttctcggcgc catgcaggtc gccgaaaacg gcgatctcgc caattggatg atccccggca
     1381 agatggtcaa aggcatgggc ggcgccatgg atctggttgg cggggtgaag aaagtcgtcg
     1441 tggtgatgga gcactcggcc aaggatgaat caaagctcaa gcatcgctgc acgctcccgc
     1501 tcaccggcgc cggcgtggtc gatatgatca tcaccgatct c
//
LOCUS       DATLJT010001690         3362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1329516,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001690 DATLJT010000000
VERSION     DATLJT010001690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329516"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VM910_38480"
     CDS             <1..284
                     /locus_tag="VM910_38480"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVI68421.1"
                     /translation="GMEVQDAVEEPRAASYSFPSSFEPHAYYPGLLNLESRIDRSTGE
                     ALGRLGHNVEWWPDWTWLAGAVCTIVADDQTGVLKAGADPRRPSYAVGL"
     gene            complement(806..991)
                     /locus_tag="VM910_38485"
     CDS             complement(806..991)
                     /locus_tag="VM910_38485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68422.1"
                     /translation="MPGMVCELGLGEALALLSMMEHDAAFPLFATEADRAMLRREATR
                     VVLDHAAKAASLPSNVP"
     gene            complement(1014..1181)
                     /locus_tag="VM910_38490"
     CDS             complement(1014..1181)
                     /locus_tag="VM910_38490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68423.1"
                     /translation="MTEPLTAGELRAVVGGIIDDVMQTDLDAVTKRIVATALKNLCRL
                     CERERRRRALH"
     gene            1433..1600
                     /locus_tag="VM910_38495"
     CDS             1433..1600
                     /locus_tag="VM910_38495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68424.1"
                     /translation="MPRLCAAQVLAFVLQARDLRTQGRELGAFIPLCLDLIGTLRIHD
                     HFKQLRTRLLG"
     gene            complement(1816..2278)
                     /locus_tag="VM910_38500"
                     /pseudo
     CDS             complement(1816..2278)
                     /locus_tag="VM910_38500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087397.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2514..2888)
                     /locus_tag="VM910_38505"
     CDS             complement(2514..2888)
                     /locus_tag="VM910_38505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68425.1"
                     /translation="MTKLLYVEDNDDNVFMLKMRLELLGDFEVLTAEDGEKGCEMAAA
                     ERPDIILMDLEMPVVDGWEATRRLKGNPQTREIPIIALSAHALAGEREKALAAGCNEF
                     DTKPIEFDRLVATVRRILADRK"
     gene            complement(2885..>3362)
                     /locus_tag="VM910_38510"
     CDS             complement(2885..>3362)
                     /locus_tag="VM910_38510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVI68426.1"
                     /translation="GYLTKPIDRERLHRLVSRFRAPVPPTRVLLVEDDAVQRERMRGW
                     LEGPQWTVREAANGREALDRLKEGKPDVILLDLMMPEMDGFAVVAALQKEAGWRDIPV
                     IVITARDLDAKDRQRLNAGVQFVLVKERFQPADLVERIRRLVRTKPAVSNEMEAAS"
BASE COUNT          613 a         1081 c         1010 g          658 t
ORIGIN      
        1 tcggcatgga ggtgcaggac gcggtcgaag agccgcgcgc cgcgtcctac agtttcccgt
       61 cctcgttcga gcctcacgcg tattaccctg gcctgctcaa cctggagagc cggattgacc
      121 ggtcaacagg agaggcactc ggccgccttg gccacaacgt cgaatggtgg cccgactgga
      181 catggctggc tggcgccgtt tgcacgatcg ttgccgatga ccagaccggc gtgctcaaag
      241 ccggcgccga cccgcgtcgg ccgtcctatg cagtcgggtt gtgaccgggc ctccacctcg
      301 ccgatcgccc tcggaacgtc ggtcctcata ccagcgagct ttgccacctt actccagcgg
      361 acgatttcgg agtcgatgaa acgctgctga gcatagcaag agcggtcgtt gaagtcgtcg
      421 cgatctgacc gcgttggggt caaaaacgga aaatcttaga gcagttgtcc actttacccc
      481 cgaaagcgga catcgcgatc cacttcgata tgtccgcttt gtaccgggag cggccgagtc
      541 atcctgaaga cgacctgagg acacatgagg caatgagagt gccaacgctt atcatcagtt
      601 cgcgagcgct tcctcgaaga catttcgggt ccgtggatgc cgcggagatc gggagggatt
      661 gaagacgcgc gtttctttca tcagcaagcc aactggtgtt accaactcgc atggcagtgc
      721 tttgatctgg ccgtggcgta taagctaacg cgcgatatgc gttgaatgcc gcaaactcgc
      781 gctggggcag cctctacgat gcactctatg gcacgttgct tggcaacgac gcagccttcg
      841 ccgcatggtc aagcaccaca cgcgtggcct ccctgcgcag catggcgcga tcggcctcgg
      901 tagcaaacag cggaaaggcc gcgtcatgct ccatcatcga gagcagcgcc aacgcttcac
      961 ctaggccaag ctcgcaaacc attccaggca tgtgcttctc agtgcttcct ctctcaatga
     1021 agcgcccgac gacgacgctc gcgctcgcaa agcctgcaca ggtttttgag cgcggtcgcc
     1081 acaatgcgct tggtgacggc gtccaaatcc gtttgcatca cgtcgtcgat gatgccccca
     1141 accacggccc gcaattcgcc cgccgtgagc ggctcggtca cggacatcaa tattccctcg
     1201 cgttcaaccg actaagccag cctagcactg gcgcgccatc acttccgggc gcgggcgcgt
     1261 aagcattgcc accgtcagag cccccactat tccgccaccg atgccaagct tgaccgtgcg
     1321 gagtggaatc ccaattctgc ggcggcgatg ctgctggcgc aggcgcagac ggtggcgtcg
     1381 gtttcgcctc aggggccgcc ttcggcatcg gcttgttcga cggtggcggg agttgccgcg
     1441 gctttgtgcc gcgcaggtgc ttgcattcgt cctgcaagct cgcgacctgc gcacgcaagg
     1501 ccgtgagctc ggcgctttca tcccgctctg cctcgacctg atcggcacgc ttcgcattca
     1561 tgatcatttc aaacagcttc gcacgcgcct gctcggctga caccgttcgc gacgcctcgg
     1621 tgcgcaccac gggcggcaac agcgccagcg cacggacgac ctccgcgagg ttgccggcaa
     1681 ctagggccac agggcgattt ttggcaatgg gatccgagag tgatgaggag cagatccgcg
     1741 gcggaatcga ggtgtgcaga gggcggcggc ggatgacttt ttcaggggtc gacctcggct
     1801 gcgggcgcgg gggatgacac ctgcgctgga gatgatcggg ccgatccccg gcacgctcat
     1861 caagcgctca cagccggcat cccggcgtgc aagaacctcg atctcactcg atagaccctc
     1921 gatccgctca tcgagccggc gccagtctcc tgccaggtcc tcgatcaccc cgcaccatgc
     1981 gcggcgacaa gacatcgcag ggcgtggcga gaatgcgtgg caactcagcg cgcaggaagc
     2041 gtaagccttg ccgaacggca acgccacgct ccaacaggaa ggcgcggatc tgattgatga
     2101 tgccggtgcg ctgactgacc aaccgctcac gcacgcggtg aagtgcctgc aaatcgagct
     2161 gctcggcagt cttggtcgcg acgaacttca tcgtcgggcg ctgcaccgcc tcggcgatgg
     2221 cctccgcatc gcggaagtcg ttcttctgtc ccttcgaata cgggcgcacg tacttcgccg
     2281 atcgtcttta tctttatctt ttcgatcccg gcatcttcgg caagcctgtc gaattcgctg
     2341 aacaattgat tgaggcaggc gacggggatg ccactgcgaa tgagcggtga tcatcccttt
     2401 gaaatcacga ccgtattaag tggccgctgg gaacaatctg gggtgaacag gaggccccag
     2461 tgacttagcg gaacctgcca ttttttcgat ggcggatgtt ccacctgaga gcgtcatttg
     2521 cggtcggcga gaatgcgccg aacggttgct actagccgat cgaattcgat cggtttggtg
     2581 tcgaactcat tgcaccccgc cgcgagtgcc ttttcgcgct cgccggcgag cgcatgcgcg
     2641 gagagggcga tgatggggat ctcgcgggtt tgtggattgc ccttgagcct ccgcgtagct
     2701 tcccagccgt cgacgacggg catttcgagg tccatcagga taatgtccgg ccgctcggca
     2761 gcggccatct cgcagccctt ctcgccgtcc tccgccgtca gcacctcgaa atcgccgagc
     2821 agctcgagcc gcatcttcag catgaagaca ttgtcatcgt tgtcttcgac gtaaagcaat
     2881 ttggtcacga tgctgcttcc atttcgttgc tgaccgcagg cttcgtccgc acgagccggc
     2941 ggatgcgctc gaccaggtcg gccggttgga atcgttcctt gaccagcacg aattggacac
     3001 cggcattcaa tcgctggcgg tctttggcat cgagatcccg cgcggtgatg acgatgaccg
     3061 ggatgtcccg ccagcccgcc tccttctgca gggcggcgac gaccgcaaag ccgtccatct
     3121 ccggcatcat cagatcgagc aggatcacgt ccggcttgcc ttctttgagg cgatccaacg
     3181 cctcgcgacc gtttgctgct tctcgcacgg tccattgcgg accctcgagc catccgcgca
     3241 tccgctcgcg ctggaccgcg tcatcctcca ccagcaggac gcgcgtgggc ggcaccggtg
     3301 cccgaaagcg gctaaccagc cgatgcagcc gctcgcggtc gatcggcttg gtgagatacc
     3361 cg
//
LOCUS       DATLJT010001691         8091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2018519,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001691 DATLJT010000000
VERSION     DATLJT010001691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018519"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VM910_38515"
     CDS             complement(<1..254)
                     /locus_tag="VM910_38515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVI68427.1"
                     /translation="MAKLSLPVRLALLVAGTTLPLIAFAGGIVFHDYVQDRKDATQRV
                     LENVRSIRFTLDAEIRRMTGGLQVLALTNALRSGDFESFDG"
     gene            complement(459..1727)
                     /locus_tag="VM910_38520"
     CDS             complement(459..1727)
                     /locus_tag="VM910_38520"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVI68428.1"
                     /translation="MSAPTSASPESAGMSSPALARVEAHLKQRYIEAGRFPGTQLLLY
                     RRGKVVHNSVQGFADLERKAPVKHDTIFRIYSMTKPITSVAFMMLFEEGRVALDEPVH
                     KYIPEWKNLGAFVAGTAPAFLTRPLSRPMLIVDLLRHTSGLTYGFQQRSNVDAAYRDK
                     KIAESGTLQSMVEELGNIPLEFSPGEAWNYSVSTDVIGYLIGKISGRPFEQFLQERIL
                     DPLGMNDTGFFVPADKASRFAACYSADPPGRVTLHATERKSTLTLQDDPAKSPFLAKP
                     SFVSGGGGLCSTAADYLTFCRALIHGGELGGVRLLGPKTLKLMTTNHLPGGVDLPALS
                     RSLFSEATYNGMGFGLGFAVNLDPAKTLIPGSAGEYNWGGLATTSFWIDPAEELIAIF
                     MTQVMPSTAYPVRRELRTMVYTAITDSNLQ"
     gene            1863..2780
                     /locus_tag="VM910_38525"
     CDS             1863..2780
                     /locus_tag="VM910_38525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HVI68429.1"
                     /translation="MPQFSEADLTQAVVKSFENTPDPRAKFLLQELVKSLHDYVRRTD
                     LTFEEWEYAIDFLTRTGQKCTAIRQEFILLSDVMGVSMLVDAVNHRERDGATETTVLG
                     PFYVGEHKEMPHGTDISPQNVTGEKMFVQSRVTDLKGKPLAGVPVDVWHADDDGFYDS
                     QKPSYATEGPSSRARFVTDSDGRFFFRTILPCSYPIPTDGPVGEMIVQTKRHPMRPAH
                     VHFLVAAKGYEPLITHVFIGGDKYLDSDVVFGVKDELVAKIEKRSDPATPDGKPVPSP
                     WHLMTYEFRLKPGGGAAPKPMAARATEDA"
     gene            3104..4486
                     /locus_tag="VM910_38530"
     CDS             3104..4486
                     /locus_tag="VM910_38530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVI68430.1"
                     /translation="MSNQSPAWISDWRPEDETFWNSRGKFIARRNLIWSIVAEHIGFS
                     VWLIWSIVATKLPQAGFHYSTDELFQLVALPGLIGSLMRFPYTFAVTTFGGRNWTIFS
                     AAVLFIPTLSLAYFVGQPDTPFWLMLLVASTAGLGGGNFASSMANISFFYPDRMKGWA
                     LGLNAAGGNIGVSSVQLLTPILMGIGLINLYQATPVGGVYLQNAGLMWVLPLAIALFG
                     AIFFMNNLTSAKSSIKDQLAIVKRKHTWIMSFIYIGTFGSFIGYSAAFPLLIKTQFPA
                     ITIAIAFLGPLVGSLARPLGGLLADKVGGAIVTFWNFIAMAAATIGVLYFVGNKDFIG
                     FLVMFLILFVTTGVGNGSTYRMIPSIFREENLRKARGKGDAARAAALKAASIESGAAL
                     GFIGAVGACGGYLIPSGFGKSIAITGGPQLALVIYLTFYAICLAMTWWFYLRRGPVTE
                     GAASLAEARV"
     gene            4578..5003
                     /locus_tag="VM910_38535"
     CDS             4578..5003
                     /locus_tag="VM910_38535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HVI68431.1"
                     /translation="MTPDQVKLVQQSFAKVAPMADQAAVMFYDRLFEIAPAVKSLFPA
                     DMSEQRRKLMATLTIVVNGLSNLEQILPAVAALATRHVAYGARAEHYPVVGSALLWTL
                     EKGLAADWTAEVAEAWTAAYTTLSGFMINQAYGRPQAAE"
     gene            5013..6227
                     /locus_tag="VM910_38540"
     CDS             5013..6227
                     /locus_tag="VM910_38540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI68432.1"
                     /translation="MSEPLVIVGNGMAAVRLVDELVTTALGRYAIAVIGEEPRLAYNR
                     VLLSSVLAGETASHDIELKPAAWWRDRGVTLKYGCRATEIDVGRRELKIEGDESIEFS
                     RLVLATGSSPLRLNVPGAELNGVHTFRDSRDVDLLLSLAARKKPVVVIGGGLLGLEAA
                     YGLAKAGTPVTLLHLMDRLMERQLDAPAADLLKSLVERKGIAVELNANTARIHGTSRV
                     EGVELADGRRIEADAVVFAAGIRPNTKLAQEAGLTVNRGIVVDDVMQTSAPGIFALGE
                     CAEHRGVCYGLVEPAYEQARVLARHLSGRNAAYQGSVVATNLKVSGVSVFSAGDFLGS
                     DASEAILLSDIRRGTYKKLVIADGKLTGAVLVGDVGDALWYLELIRTRNPIAAIRKEM
                     MFGRALAVPKAA"
     gene            6241..>8091
                     /locus_tag="VM910_38545"
     CDS             6241..>8091
                     /locus_tag="VM910_38545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVI68433.1"
                     /translation="MTSTRTTCPYCGVGCGVLATPDGKGGAAISGDPDHPANLGRLCS
                     KGSALGETLSLESRLLHPMIRCSKGTMERVAWSDALDHVAHRLKHIIARDGAKAVAFY
                     LSGQMLTEDYYVANKLMKGFIGSANVDTNSRLCMASSVAGHRRAFGADTVPGCYEDLD
                     EADLLVLVGSNAAWCHPVLFQRMLANKQTRGARIVVIDPRRTDTAGDAELFLGLKPGT
                     DTALFSGLLVHLADNGALDRAYIEGHTIGFDEALARARNIAGSVAATALATGLSERDV
                     AAFFEMFLRNERVVTLYSQGVNQSAQGTDKVNAIINCHLATGRIGKRGAAPFSLTGQP
                     NAMGGREVGGLANQLAAHMAFTPQDIDRVRRFWKAPRIATHEGLKAVQMFEAIARGEI
                     KALWVIGTNPAVSLPDADAVREAMKKLELLVVSENVRDNDTVNVGAHVLFPALAWGEK
                     SGTVTNSERRISRQRAFMPAPGEARPDWWILSEVAKRLGFGGAFDYKSAADVFREHAA
                     LSAFENVGGRDFDIGALQKVGDDAYDEMLPLHWPMRWGDLEPRARFFFDGGYYTNDRK
                     ARFVAPEVPALRTETNPGRPLRLNTGRIRDQWHTMTRTGLSPRLGAHLPEP"
BASE COUNT         1457 a         2533 c         2584 g         1517 t
ORIGIN      
        1 ccgtcgaaac tctcgaaatc gccactgcgc aacgcgttgg tcagcgcgag cacctgcagg
       61 cctccggtca tccgcctgat ctcggcatcg agggtgaaac ggatgctgcg gacattctcc
      121 agcacgcgct gcgtcgcgtc cttgcggtcc tggacataat cgtggaagac gatgccgccc
      181 gcgaaagcga tcaacggcag cgtcgttccc gcgaccagaa gcgcaagccg tacggggagg
      241 gagagttttg ccaactattt tgccaactcc tgggccttgt tttgcgaggt acggcgctgg
      301 aatttccgat cggtggcacg cgttctgaaa agcactcgcc ctgaggcaac gcctgaagtt
      361 tcgagcatag gagaagcctc caaattccgc caaggatttt ggcgctggcg attccgtctc
      421 aaggcgtcgt aaagatgcag tgcgaaaatc gaaaacgttt attgaagatt gctgtccgtg
      481 attgcggtgt acaccatggt gcgcagttcg cgcctgactg gataggcggt cgacggcatc
      541 acctgggtca tgaatatggc gatcaactct tccgccggat cgatccagaa tgatgtcgtc
      601 gccaaaccgc cccaattgta ttcgccggcg ctgccgggga tcagcgtctt ggctggatcc
      661 agattgacgg caaagccaag accgaatccc atgccattat aggtcgcttc tgaaaacagc
      721 gagcgtgaca gggcgggcag gtcgaccccg ccgggcagat ggttcgttgt catcagcttc
      781 agcgttttcg gtcccaatag cctgacgccg ccaagttcgc cgccgtggat cagcgcgcgg
      841 cagaaggtga gatagtcggc tgcggtcgag cacagtccgc cgccgcccga gacgaacgac
      901 ggctttgcaa gaaacgggct ctttgccgga tcgtcctgca gcgtcagcgt gctcttgcgc
      961 tcggtggcgt gaagggtgac gcggcccggc gggtcggccg agtagcaggc ggcgaaccga
     1021 ctggccttgt cggccggcac gaagaagccg gtgtcgttca taccgagcgg atccaaaatc
     1081 ctctcctgca ggaactgctc gaaaggccgg cctgaaatct tgccgatcag atagccgatc
     1141 acgtcggtgg aaaccgaata gttccaggct tctccgggcg aaaattcgag tgggatgttg
     1201 ccgagttcct cgaccatgct ttgcagcgtc ccgctttccg cgatcttctt gtcgcggtag
     1261 gcggcgtcca cgttggagcg ctgctggaag ccgtaggtca ggcccgaggt gtggcgcaac
     1321 agatcgacga tcagcatcgg gcgcgacaaa ggcctggtga gaaaggctgg cgcagtgccg
     1381 gcgacgaacg cgccgagatt cttccactcc gggatatatt tgtggacggg ctcgtcgagc
     1441 gcgacgcggc cttcctcgaa cagcatcatg aaggccacgc tggtgatcgg cttggtcatc
     1501 gaatagatgc ggaagatcgt gtcgtgcttg acgggcgcct tgcgctcgag gtccgcaaag
     1561 ccctgcacgg aattgtggac caccttgccg cggcggtaga gcaggagctg agtgcccggg
     1621 aagcgtccgg cctcgatgta acgctgcttc agatgagctt cgacccgcgc aagggcggga
     1681 gaggacatgc cggcggattc gggtgaggct gaggtcggag ctgacatggg tcgttcccgg
     1741 gtgcggggtt cgagacatcg atagcggaaa aggcgtttct gttccaatca catgcgctta
     1801 ctcctttgcg cgcgctggtg taggctcatc gctctcgccg ccttcttccg ggaaacgcaa
     1861 aaatgccgca attcagcgaa gccgacctga cccaagccgt cgtcaagagc tttgagaaca
     1921 caccggaccc gcgtgccaaa ttcctgctgc aggagctggt gaagtcgctg cacgattatg
     1981 tgcgccgcac cgatctcacc ttcgaggaat gggaatatgc gatcgatttt ttgacccgca
     2041 ccggccagaa atgcaccgca atccgtcagg agttcatcct gctttccgac gtcatgggcg
     2101 tttcgatgct ggtcgatgcg gtcaaccatc gcgagcgtga cggcgcgacc gagaccacgg
     2161 tgctcggacc gttctatgtc ggagagcaca aggagatgcc gcatgggacc gacatctcgc
     2221 cgcagaacgt gaccggcgag aagatgttcg tgcagagccg cgtcaccgat ctcaagggca
     2281 agccgttggc cggcgtgccc gtcgacgtct ggcacgccga cgacgacggc ttctatgact
     2341 cgcagaagcc gtcttacgcc accgaagggc cgtcatcgcg ggcgcgcttc gtcaccgaca
     2401 gcgacgggcg cttctttttc cgcaccatcc tgccgtgcag ctatccgatc ccgaccgatg
     2461 gtccggtcgg cgagatgatc gtgcagacca aacgccatcc gatgcggcct gcgcatgtgc
     2521 atttcctcgt cgccgcgaaa ggctacgagc cgctcatcac ccacgtcttc ataggcggcg
     2581 acaaatacct cgactccgac gtggtgttcg gcgtgaagga tgaactggtc gcgaaaatcg
     2641 agaagcgcag cgatccggcc acgccggacg gcaagcccgt cccgtcgccc tggcatctga
     2701 tgacttacga attccgtctg aagccgggtg ggggagcggc gccgaagccg atggccgcgc
     2761 gcgcgaccga ggacgcgtaa cgaggctgat ttggactgct cgtgttgcag cgggcttaat
     2821 ttttaatcct tgtgctcagt tcttgtgcgc cgcacaagaa ataagcgaat cgcaaatttt
     2881 gaatgcgaat ccgctctgct gatgtttcag gcaatgtttt ataactcgct gtaatagcag
     2941 aactttcttt gacgtggcgg cttggcacgg agcttgaata gctcctgtcg gacgccaccg
     3001 acgccgtccc ccgacagcaa tcatccgaat ctcgagaccg cggctggggc ttccccggaa
     3061 cgcggctgcg tggccgccga cgaccagcga aaggacctta cgtatgagca atcagagccc
     3121 ggcctggatt tcagactgga gacccgagga cgagacgttt tggaattcca ggggcaagtt
     3181 cattgcccgc cgcaatctga tctggtcgat cgtggccgag catatcgggt tttcggtctg
     3241 gctgatctgg agcatcgtcg cgaccaagct gccgcaggcc ggcttccact attccaccga
     3301 cgaactgttt cagctcgtgg ccctgcccgg tctgatcggc tcgttgatgc gcttccccta
     3361 cacctttgcg gtgaccacct tcggcggccg caactggacc atcttctcgg cggctgtcct
     3421 gttcattccg acgctgtcgc ttgcctactt cgtcggccag cccgatacgc cgttctggct
     3481 gatgctgctg gttgcctcca ccgccgggct tggcggcggc aactttgcgt cctcgatggc
     3541 caacatctcc ttcttctatc cggaccggat gaagggttgg gcgcttgggc tgaacgcggc
     3601 cggcggcaat atcggcgttt ccagcgtgca gcttctgacg ccgatcctga tgggcatcgg
     3661 cctgatcaat ctctatcagg caacgccggt cggcggcgtc tacctgcaga atgcgggact
     3721 gatgtgggtg ctgccgctcg cgatcgcatt gttcggcgcc atcttcttca tgaacaatct
     3781 gacaagcgca aaatcctcga tcaaggacca gcttgccatc gtcaagcgca agcacacctg
     3841 gatcatgtct ttcatctata tcgggacgtt cggctccttc atcggctatt cggcggcgtt
     3901 tccgctcctg atcaagacgc aattcccggc catcacgatc gccatcgcct tcctcggacc
     3961 gctggtcggc tcgctggcgc gcccgctcgg cggcctgctc gccgacaagg tcggaggcgc
     4021 gatcgtcacc ttctggaatt tcatcgcgat ggccgccgcc acgatcggcg tgctgtactt
     4081 cgtcgggaac aaggatttca tcggcttcct ggtgatgttc ctgatcctgt tcgtcacgac
     4141 cggggtcggc aacggatcga cctatcggat gatcccctcg atcttccgcg aggaaaatct
     4201 tcggaaggcg cgcggcaaag gtgatgccgc tcgcgcggcc gcgctcaagg ccgcctcgat
     4261 cgagagcgga gccgcactcg gcttcatcgg cgcggtcggc gcctgcggcg gctacctgat
     4321 cccgagcggc ttcggcaagt cgatcgcgat caccggcgga ccgcaactcg cgctggtgat
     4381 ctacctcacc ttctacgcga tctgccttgc gatgacctgg tggttctacc ttcgccgcgg
     4441 cccggtgacg gagggtgcgg ccagcctcgc tgaagcgcgc gtttaagact cgcatgcacg
     4501 aggcgcgagt ctttgcgcct ttcgccatca atccttcgcc ccgagggcga aggaccaacg
     4561 aagagaagga gcgaagcatg acacccgatc aggtcaaact cgtgcagcaa agctttgcca
     4621 aggttgcacc gatggccgat caggcggcgg tgatgttcta cgaccgcctg ttcgagattg
     4681 cgcccgcggt gaagtcgctg ttccccgccg acatgagcga gcagcgacgg aaactgatgg
     4741 cgacgctcac gatcgtcgtg aacggacttt cgaacctcga gcagatcctg ccggcggtcg
     4801 ctgcgctggc cacgcgccat gtcgcctacg gtgcaagggc cgagcactat cccgtggtcg
     4861 gctcggcgct tctgtggaca ctcgagaagg gactggctgc ggattggacc gccgaggtcg
     4921 ccgaagcctg gaccgccgcc tataccacgc tgtccggctt catgatcaac caggcctatg
     4981 gccggccaca ggcagccgag tgaggacttg ccgtgagcga accgctggtc attgtcggca
     5041 acggaatggc cgccgtgcgc ctggtcgacg aactcgtgac cacggcgctg ggccgttacg
     5101 ccatcgcggt gatcggcgag gagccgcggc ttgcctataa tcgcgtactg ctctcgtcgg
     5161 tgctcgccgg cgaaactgcc tcccacgaca tcgagctcaa gccggccgcg tggtggcgcg
     5221 accgtggcgt cacgcttaag tatggctgcc gtgcgaccga aatcgacgtc ggccggcgcg
     5281 agctgaagat cgaaggcgac gagagcatcg agttctcgcg tctcgtgctg gcgaccggat
     5341 cctcgccgct ccggctcaac gtgccgggcg cagagctgaa cggcgtgcac acgttccgcg
     5401 acagccgcga tgtggacctc ctgctctcgc ttgccgcgcg gaagaagccc gttgtcgtga
     5461 tcggcggcgg attgctgggg ctcgaggcgg cctacggcct tgccaaggcg ggaacaccgg
     5521 tcaccttgct gcatctgatg gaccgcctga tggaacgtca gctcgatgcg ccggcggccg
     5581 atctgctgaa atcgctggtc gagcgcaagg gcatcgcggt cgagctgaac gccaatactg
     5641 cgcgcatcca tggcacgagc cgcgtcgaag gcgtcgagct tgcggacggc cggcggatcg
     5701 aggcggatgc agtggtgttt gccgccggca tccggccgaa cacgaagctc gcgcaggagg
     5761 cgggcctcac cgtcaaccgc ggcatcgtgg tcgacgatgt catgcagacc agcgcgcccg
     5821 gtatctttgc gctcggcgaa tgcgccgagc accgcggcgt ctgttacggg ctggtcgagc
     5881 cggcctatga gcaggcgcgc gtgctggccc ggcatctttc cggacggaac gcggcttacc
     5941 agggcagcgt ggttgccacc aatctgaaag tctcaggggt cagcgtgttc tccgcgggtg
     6001 atttcctggg aagcgacgcc agcgaagcga tcctgttgag cgatatcagg cgcggcacct
     6061 acaaaaagct ggtcattgcg gacggaaagc tcaccggcgc ggtgctggtc ggcgatgtcg
     6121 gcgatgcgct gtggtatctc gagctgatcc gcacccgcaa tccgatagcc gcgatccgca
     6181 aagagatgat gttcggccgc gcgctcgcag ttccgaaggc ggcatagcgg tgaggcgcgc
     6241 atgacctcaa cccgcaccac ctgcccctat tgcggagtcg gctgcggcgt gttggcaact
     6301 cccgacggca agggcggggc ggcgatctcg ggcgatcccg atcatcccgc caatctcggg
     6361 cggctctgct cgaagggctc ggcgctgggt gagacgcttt cgctcgaaag ccggctgctc
     6421 cacccgatga tccgctgcag caagggtact atggagcggg tggcgtggag cgacgcgctc
     6481 gatcatgtcg cgcaccggct caagcacatc atcgcccgcg acggtgcgaa agcggtcgcc
     6541 ttttatctat ccgggcagat gctgaccgag gattactacg tcgccaacaa gctgatgaag
     6601 ggatttatcg gctcggcgaa tgtcgacacc aattcgcggc tatgcatggc gtcgtctgtc
     6661 gcaggccacc gccgcgcgtt cggcgccgac accgtgccgg gctgctacga ggatctcgac
     6721 gaagccgatc tgttggtcct cgtcggctcc aacgcggcct ggtgtcatcc ggtgctgttc
     6781 cagcggatgc tcgccaacaa gcagacacgc ggcgcgcgca tcgtcgtgat cgatcctcgc
     6841 cgcaccgaca ccgccggcga cgccgagttg ttcttgggcc tcaaacccgg caccgatacc
     6901 gcgctgttct cgggcctcct cgtgcatctc gccgacaacg gcgcgctcga ccgtgcctat
     6961 atcgaggggc acaccatcgg tttcgacgag gcgctggcgc gagccaggaa tatcgccggc
     7021 agcgtcgctg cgaccgcgct cgcgaccggc ctgtcggaac gtgatgttgc ggcgttcttc
     7081 gagatgttcc tcaggaacga acgcgtggtg acgctctatt cgcaaggcgt caatcaatcg
     7141 gcgcagggca ccgacaaggt caacgcgatc atcaattgcc atctcgccac cggacgcatc
     7201 ggcaagcgcg gcgccgcccc gttctcgctg accggccagc ccaatgcgat gggcggccgc
     7261 gaggtcggcg ggctcgccaa ccagcttgcc gcccacatgg ccttcacgcc gcaagacatc
     7321 gaccgcgtgc ggcggttctg gaaagcgccg cgcatcgcca cccatgaggg gctgaaggcg
     7381 gtccagatgt tcgaggcgat cgcgcgcggc gagatcaagg cgctgtgggt gatcggcacc
     7441 aatcccgcgg tgtcgctgcc ggatgccgac gcggtgcgcg aggcgatgaa gaagctcgaa
     7501 ctgctggtgg tgtcggagaa tgtgcgcgac aacgacaccg tcaatgtcgg cgcgcatgtg
     7561 ctgttccccg cgctcgcctg gggcgaaaag tcgggcaccg tgaccaattc ggagcggcgc
     7621 atctcgcgcc agcgcgcgtt catgcccgcg cccggcgaag ccaggcccga ttggtggatc
     7681 ctgagcgagg tcgcaaagcg cctcggcttc ggcggcgcgt tcgactacaa atcggcggcg
     7741 gacgtgttcc gcgagcatgc ggcgctatcg gcgttcgaga acgtcggcgg ccgtgacttc
     7801 gacatcggcg cgttgcaaaa ggttggcgac gacgcatacg atgagatgct gcctttgcac
     7861 tggccgatgc gatggggcga cctcgagcca agggcgcgct tctttttcga cggcggctac
     7921 tatacgaacg accgcaaggc ccgcttcgtg gcgccggaag ttccagcgct gcggaccgag
     7981 accaatcccg gccgcccatt gcgactcaac accgggcgca ttcgcgacca gtggcacacc
     8041 atgacccgca ccggcctcag tccaaggctg ggcgcgcatc tgccggagcc g
//
LOCUS       DATLJT010001692         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2476894,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001692 DATLJT010000000
VERSION     DATLJT010001692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2476894"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VM910_38550"
     CDS             <1..735
                     /locus_tag="VM910_38550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611274.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVI68434.1"
                     /translation="CNKFLAKIASDLDKPRGFAALDQDEACAMLAEKPVGFIFGVGPA
                     TQERLVSRGFRTIGDLQRADETELMRQFPTDGRRLWRLARGSDDRRVVPDRGAKTISS
                     ETTFESDINDFATLERLLWRLSEKVSARLKTGELAGCTITLKLKTADFRQRTRSQSIA
                     SPTQLAAKIFAVSREMLAKEIDGTAFRLMGTGVSALRPGSRADDSDMLDRRAAHAERA
                     IDDLRRKFGASAVIRGIAYDGPEKAE"
     gene            complement(749..1594)
                     /locus_tag="VM910_38555"
     CDS             complement(749..1594)
                     /locus_tag="VM910_38555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HVI68435.1"
                     /translation="MAQRWRISLGLLAIAAVACGPAKAAATAAFLPHQALYELSLLKS
                     RGSSPVNSARGRILYNFSGSACEGYTSEFRQVSELDVGEGKVTLSDLRSSSWEDGAGK
                     TYRFQIETRMNDADSNPIDGIAERTGDHITVKLKKPETKTFTLDGSTVFPTEQIQRII
                     AAAREGKSVLELTVYDGSDNGEKVYNTLSVIGQPIPGDKTIASPDPSTTNEQMKSLTR
                     WPVTVSYYDHDGKSKDGEQTPVYAMSFELFENGVSRALVLDYNDFVISGALGKFDVKD
                     AKPCN"
     gene            1806..2273
                     /locus_tag="VM910_38560"
     CDS             1806..2273
                     /locus_tag="VM910_38560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVI68436.1"
                     /translation="MAGSIEAKLAQLGITLPQPVAPVANYVGFVRSGNMLFVSGQICV
                     DAQGKLIAKGKLGAGVSTEQGAAAARGCGINLLAQAKAALGDLDKVVRVVRLGGFVNS
                     APDFLDGPKVLNGASDLMVAVFGDKGRHARTTVGVAVLPADASVEVEAVFEVA"
     gene            2276..3028
                     /locus_tag="VM910_38565"
     CDS             2276..3028
                     /locus_tag="VM910_38565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HVI68437.1"
                     /translation="MHAPEWLTARPVAHRGLHDASRGIIENMPGAAQAAISGNFAIEC
                     DIQLTSDGEAMVHHDDELGRLTEGSGALLGKTAAELKAVSFKGTAERMMTLSDLCTLV
                     AGRVPLVIEIKSHFDGDRRLVTRMAEVLSSYTGPAVGMSFDPDQVMALRELIPSRPRG
                     IVAERHYTAEDWPETTRAQRWEMKHLHHFFLTQPDFVAYWIDELPSPAPWIARNIFGR
                     PLLTWTVRTPEQRARAARHADQMIFEGFRPEA"
     gene            3126..>3408
                     /locus_tag="VM910_38570"
     CDS             3126..>3408
                     /locus_tag="VM910_38570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HVI68438.1"
                     /translation="MASPEITLEAVPSAGEIPAEAWDACANPGANRHHFNGATPNASC
                     ANSRPYYNPFVSHAFFSAAEKSGSAAVRTGWGPRHLIAKLDGAIAGIVPC"
BASE COUNT          620 a         1064 c         1088 g          636 t
ORIGIN      
        1 tgcaacaagt ttctggccaa gatcgcctcc gacctcgaca agcccagggg ctttgccgcg
       61 ctcgatcagg acgaggcgtg cgcaatgctc gcagaaaagc cggtcggctt catcttcggc
      121 gtggggccag cgacccagga acggctggtt agtcgcggct ttcgcaccat cggagacctg
      181 cagcgtgcgg acgagaccga gctgatgcgc caattcccga ccgatggccg caggctgtgg
      241 cggctggcgc gcggcagcga cgatcgccgc gtggtgcctg accgaggtgc gaagacgatt
      301 tccagcgaga cgaccttcga gagcgacatc aacgatttcg ccacgctcga acggctgttg
      361 tggagattgt cggaaaaggt ctccgcgcgg ctcaagaccg gcgagcttgc gggttgcacg
      421 attacgctca aactgaaaac ggccgatttc cgccagcgca cacgctcgca atcaattgcc
      481 tcaccgaccc agctcgcggc caagatcttc gccgtctctc gcgagatgct ggcgaaggag
      541 atcgacggca cggcgttccg cctgatgggt accggcgtca gcgcgctgcg gccgggatcg
      601 cgggccgacg acagcgacat gctcgatcgc cgcgcagcgc acgcggaacg cgcgatcgat
      661 gatttgcgca ggaaattcgg cgcgagtgcg gtgatccgcg ggattgccta tgacgggccg
      721 gaaaaggcgg agtgacgccc ttcgggcgtc agttgcacgg tttcgcgtcc ttgacgtcga
      781 atttgccaag cgcgcccgaa atcacaaagt cgttgtaatc gagcaccagg gcgcgcgaca
      841 cgccgttctc gaacagctcg aacgacatgg cgtagaccgg cgtctgctcg ccgtccttcg
      901 acttcccgtc gtggtcgtaa tagctcaccg tgaccggcca gcgcgtcagc gatttcatct
      961 gttcgttggt ggtggatgga tcgggcgaag cgatcgtttt gtcgccgggg atcggttggc
     1021 cgatcaccga gagcgtgttg taaaccttct cgccattgtc cgagccgtcg taaacggtca
     1081 gctcgagcac ggattttcct tccctggcgg ctgcgatgat gcgctggatc tgctcggtcg
     1141 ggaacacagt cgagccgtcc agcgtgaagg ttttcgtctc gggctttttc agtttgaccg
     1201 tgatgtgatc cccggtcctt tcggcgatgc catcgatcgg attggagtcg gcatcgttca
     1261 tccgtgtctc gatctggaat cggtaagtct tgccggcacc atcctcccag gagctcgaac
     1321 gcaggtcgga tagcgtcacc ttgccctcgc cgacatcgag ttccgaaacc tgccggaatt
     1381 ccgaggtgta gccctcgcag gcgctaccgg agaaattgta gaggatgcgt ccccgcgcgc
     1441 tgttgaccgg gcttgagccg cgcgacttca ggagactgag ttcgtaaagc gcctgatgcg
     1501 gcagaaacgc cgcggtggcc gcggccttgg ccggtccgca cgcgactgct gcgatggcca
     1561 gcaaacccag ggaaatccgc caacgttgcg ccatgactta tccttggatg gggaaaatcg
     1621 ggacattaat gaccggccca ttgcgtcgca actggggccg cttcacggaa acgtgcgaat
     1681 tttgcggcta cgcccggcgt cccggcggca cccctgccct cgcttgcatg tcatggcgcg
     1741 atgcgcgaaa cagggccgct cgatctgtat cgatcgggtt tatcaatcaa gtcggggtta
     1801 aagacatggc aggttcgatc gaagcaaaat tggcgcaact cggcatcacc ttgccccagc
     1861 ccgtcgcacc ggtcgcaaat tatgtcggct ttgtgcgcag cggaaacatg ctgttcgtct
     1921 ccggccagat ctgcgtcgac gcgcaaggca aactgattgc caagggcaaa ctcggagccg
     1981 gcgtcagcac cgagcagggt gccgccgcgg cccgtggttg cggcatcaac ctgctcgcgc
     2041 aggcgaaggc tgcgcttggc gacctcgaca aggtcgtgcg ggtggtccgg ctcggcggtt
     2101 tcgtgaattc ggcaccggat ttcctggacg gaccgaaggt cctgaacggc gcgtccgacc
     2161 tgatggttgc ggtgttcggc gacaaaggtc ggcatgctcg caccacggtc ggcgtcgctg
     2221 tgctgccggc agatgcttcg gtcgaggtcg aagcggtctt cgaggtcgcc tgaaattgca
     2281 cgctccggag tggctgacag cacggccggt cgcgcatcgc ggcctgcatg atgcctcgcg
     2341 cggcattatc gagaacatgc cgggcgcggc gcaggccgcg atatcaggca atttcgcgat
     2401 cgaatgcgac atccagctta cgtccgacgg cgaagccatg gtgcatcatg acgacgagct
     2461 cggtcgcctg actgagggat cgggcgcgct gcttgggaag accgccgccg agctgaaagc
     2521 cgtttcattc aagggcacgg ccgaacggat gatgacgctg tccgatctct gcacgctggt
     2581 ggccggccgc gtgccgctgg tgatcgagat caagagccat tttgatggcg accgcaggct
     2641 ggtgacgcga atggcggagg tgctgtcgtc ctatacagga ccagcggtcg gcatgtcctt
     2701 cgatccggat caggtcatgg ccctgcgcga gctgatcccc tcgcgcccgc gcggcatcgt
     2761 cgccgagcgc cactacaccg cggaggattg gccggagacg acgcgggcgc agcgctggga
     2821 gatgaagcat ctgcaccact tcttcctcac gcagcctgac ttcgtcgcct actggatcga
     2881 cgaactgccc tcgcccgcgc cctggatcgc ccgcaatatc ttcggccgcc cgcttttgac
     2941 ctggacggtg cgcacgccgg agcagcgcgc ccgcgcagcg cgccacgccg accagatgat
     3001 ctttgagggc tttcggccgg aagcgtaaaa cgcgaaaaaa tcattcgata cgcggctcgc
     3061 gatgacgatg cggtgaacgc gcaggccctt gcagtcgctg ctgcaatgca cgatgtttgg
     3121 ggcagatggc ctcacccgaa atcacactcg aagcagttcc ctctgccggc gagatcccgg
     3181 ccgaggcatg ggacgcttgc gccaatcccg gcgccaaccg ccaccacttc aacggcgcta
     3241 cgccaaatgc ctcctgcgcc aattcaaggc cctattacaa cccattcgta tcccacgcat
     3301 ttttctcggc tgcagaaaaa tcggggtcgg cggccgtacg caccggctgg ggcccacgcc
     3361 atctgatcgc gaagctcgat ggtgcgatcg caggcatcgt gccctgct
//
LOCUS       DATLJT010001693         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1558958,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001693 DATLJT010000000
VERSION     DATLJT010001693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558958"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VM910_38575"
     CDS             complement(<1..411)
                     /locus_tag="VM910_38575"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVI68439.1"
                     /translation="MTWSMPNFAIARPRDIGSAISAREQESSSLFLAGGTDLLVNLRL
                     GLGRPKLLIDLSAIDALHEKSFAATGVRIGACVSLMELIDLPAIRQRYRALAEAAEAV
                     ASPTTRSIATIGGNLCLQTRCVYYNQSEWWRRANN"
     gene            complement(408..2738)
                     /gene="hcrA"
                     /locus_tag="VM910_38580"
     CDS             complement(408..2738)
                     /gene="hcrA"
                     /locus_tag="VM910_38580"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HVI68440.1"
                     /translation="MTGQVPSELRHRGVPLIDGIEKVTGRARYTADLDHAGALVGRIL
                     RSPVSHAEIKRLDVTKARELDGVFAVVTGADCPHTYGVLPVAMNEYPLARDRVRYRGE
                     PIAAVAAVDAATAEQALDLIEFEMRELPAYYSSAAARADGATLLHENKPGNIEREVHH
                     VFGDVAAGFDRADLVGEHRISCSEVNHAQIEPHAILAEFDPQTQRLTVQSVTQVGYYL
                     QLMLARCLDMDVSRIRVIKPFIGGGFGARVEVLNFEIIAALLARAVAGTVMMQLTREE
                     NFLTHRARPHTDVTLKLGMRQSGQITACACEVVQRGGAYAGYGIVTILYAGALLQGIY
                     DIPAVKFDGYRIYTNLPPCGAMRGHGSVDVRHAFECVLDRMARELGLDPFAVRRANLL
                     DVPTRTMNQLVVNSCGLSECLDRVEHASDWRARIGRLPPGKGLGMACSHYVSGAAKPI
                     HWTGEPHAVVNLKLDFDGGITAFTGAADIGQGSSTMVAIAVAETLGVSLERVRVVAAD
                     SAITPKDNGAYSSRITFMVGNAAIDAAKKLRAILIEAAGRKLDARPDDIECIGEQFLI
                     GKGGQASLSLAEVVKEALVGSGAIQVKGTFTCPPDAQGGKHKGGAVGSTMGFSYAAQV
                     VEVTVDEPTGAITVDHVWTALDCGHAINPLAVEGQIQGSVWMGMGQALSEETHYLDGL
                     PAHSNLLEYRVPTIVESPPIEVQIVEARDPSGPFGAKEASEGALASFPPAFVNAVANA
                     IGIDFDQLPLTPDRVMQAMIERRRQARASALKGHAS"
     gene            complement(2735..>2878)
                     /locus_tag="VM910_38585"
     CDS             complement(2735..>2878)
                     /locus_tag="VM910_38585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVI68441.1"
                     /translation="MAAEALLRRNSRPSKEEIKAALAGNICRCTGYVKIIESVHAAAE
                     ATS"
BASE COUNT          531 a          899 c          894 g          554 t
ORIGIN      
        1 attgttggca cgacgccacc attcgctttg gttgtagtag acgcatcgtg tctgcaggca
       61 aagattgccg ccgatggtcg cgatgctccg cgtcgtcggg cttgcaacgg cctccgcggc
      121 ttccgcaagc gcgcgatagc gttgacggat cgctggcagg tcgatgagct ccatcaggct
      181 cacgcaggca ccaatccgga ccccggtggc ggcgaatgat ttttcgtgca gtgcgtcgat
      241 ggcggacaaa tcgatgagta acttcgggcg cccgaggccg agccgtaaat tgacaagcag
      301 atcggttccg ccggcgagga acagacttga ggattcctgt tcgcgtgcgg aaatcgccga
      361 gccgatgtcg cgcggccggg cgatagcaaa gttcggcatc gaccaagtca tgacgcatgc
      421 cctttgagcg cgctggcgcg ggcctggcgg cgacgctcga tcatggcttg catgacgcgg
      481 tctggcgtga gcggaagctg atcgaaatcg atgccgatgg cattcgccac cgcgttgaca
      541 aaagccggcg gaaagctcgc cagtgcgcct tcgctggcct ctttggcccc gaacggtccg
      601 gacggatcac gggcttcgac aatttgaact tcgatcggtg gtgattcgac gatggtcggc
      661 acgcggtact ccagcaaatt agaatgcgcc ggcagaccgt cgaggtagtg cgtttcctcg
      721 ctcaaggcct gacccatgcc catccacacc gagccttgga tttgtccctc gaccgccagt
      781 gggttgatcg catggccaca atcaagcgcg gtccacacgt gatcgacggt gatcgcgcca
      841 gtcggctcgt cgaccgtgac ttcgacgacc tgcgctgcgt agctgaagcc catggtcgat
      901 ccaaccgctc cgcctttgtg cttgccgcct tgcgcatcag gcgggcaggt gaatgttccc
      961 ttcacctgaa tggcgccgct tccgaccagg gcctctttca ccacctcggc aagcgacagg
     1021 ctcgcctggc cgcctttacc gatcaggaac tgctcgccga tgcattcgat gtcgtccggt
     1081 cgggcgtcga gcttgcgtcc tgcggcttcg atcagaattg ctctaagctt tttcgcagca
     1141 tcgatggcgg cattgccgac catgaaagta atgcgcgacg aataggcgcc attgtccttt
     1201 ggcgtgatcg cgctgtccgc ggccaccacg cggacgcgtt cgagtgaaac acccaacgtc
     1261 tcggcgaccg cgatcgccac catcgtagaa gagccttggc cgatatcagc ggcgccagtg
     1321 aacgcggtaa tgccgccgtc gaaatcaagc tttaggttga cgaccgcatg cggctcgccg
     1381 gtccagtgaa tgggcttggc cgcaccgctc acataatgcg agcaagccat gcccagtccc
     1441 tttcccggcg gcaatctgcc aatccgggcg cgccagtcgc tggcatgctc cacgcggtcg
     1501 aggcattcgg acaatccgca gctgttcacc acgagctgat tcatcgtccg ggtgggaacg
     1561 tcgagaagat tggcgcggcg taccgcgaac ggatcgagcc cgagttcgcg tgccatccga
     1621 tccaggacac attcgaaggc atggcgcaca tcgaccgagc cgtgaccgcg catcgcgccg
     1681 cagggcggta gattggtata gatgcgatag ccgtcgaact tgacggcggg aatatcgtag
     1741 atgccttgca gtagcgcgcc ggcgtagagg atggtgacga taccgtagcc ggcataggcg
     1801 ccgccacgtt gcaccacctc gcaggcgcag gcggtgattt gcccgctttg gcgcatgcca
     1861 agcttgagcg tgacgtcagt gtgcggacgg gcgcggtgcg tcaggaagtt ctcctcgcgc
     1921 gtcaattgca tcatcaccgt gccggccacg gcgcgcgcaa gtagggcggc gatgatctcg
     1981 aagttcagca cctcgacccg cgcgccgaag ccgccgccaa tgaatggctt gatcacgcgg
     2041 atgcgcgaga catccatgtc gagacagcgc gcgagcatca gctggaggta atagccaacc
     2101 tgcgtcacgc tctgaacggt gagtcgctgt gtttgcggat caaactcggc aagtatggcg
     2161 tgcggctcga tttgcgcatg attgacctcg ctgcagctga tccgatgctc acccaccagg
     2221 tcggctcgat cgaatcccgc tgccacgtca ccgaagacat gatggacctc gcgctcgata
     2281 ttgccgggtt tgttctcgtg cagcagcgtt gcgccatcgg cgcgcgctgc tgcggacgaa
     2341 tagtaggccg gtaattcgcg catctcgaat tcgatcaggt cgagcgcctg ctcggccgtg
     2401 gctgcatcca ccgcggccac cgcggcgatt ggctcgccgc gatagcggac ccgatcgcgt
     2461 gcaagagggt attcgttcat cgcaaccggg agcacgccat aggtatgcgg gcagtcggcg
     2521 ccggttacga cagcgaagac cccatcaagc tcccgcgcct ttgtcacatc gagccgtttg
     2581 atctcggcat ggctgaccgg actgcgaagg atgcgtccga ccaatgcccc ggcatgatcc
     2641 agatcagcgg tatagcgggc gcggccggtg actttttcga tgccgtctat caacggaacg
     2701 ccgcgatgcc gcagctccga tggcacttgg ccggtcacga cgtcgcctcc gccgcggcat
     2761 gcaccgattc aatgattttt acatacccgg tacagcggca aatgttgccg gcgagcgcag
     2821 ctttgatttc ctctttgctc ggccgggaat tgcgccgcaa cagcgcctcc gccgccat
//
LOCUS       DATLJT010001694         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2711763,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001694 DATLJT010000000
VERSION     DATLJT010001694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2711763"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VM910_38590"
     CDS             complement(<1..216)
                     /locus_tag="VM910_38590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68442.1"
                     /translation="MFQGYIDIDGALPVNATASCDVLFERGLYWASGRSGVINLVAAH
                     KWFNLAALKGRSDAIALRREVAEQMSDL"
     gene            complement(1238..1465)
                     /locus_tag="VM910_38595"
     CDS             complement(1238..1465)
                     /locus_tag="VM910_38595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68443.1"
                     /translation="MHSVEAQLSPLFVSTRGKFVSKRREASCESTSAIRINEPILWPA
                     TMHSAVNHENIFLAKFQELESMQRLLTTVGA"
     gene            complement(1466..2944)
                     /gene="gatB"
                     /locus_tag="VM910_38600"
     CDS             complement(1466..2944)
                     /gene="gatB"
                     /locus_tag="VM910_38600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVI68444.1"
                     /translation="MTAASGKLIKGATGDWEVVIGMEVHAQVTSKSKLFSGASTAFGG
                     EPNSHVSLVDAAMPGMLPVINEECVRQAVRTGLGLSAKINLRSVFDRKNYFYPDLPQG
                     YQISQYKSPIVGEGEVTLELDGGKTATVGIERLHLEQDAGKLLHDQSATMSHVDLNRS
                     GVALMEIVSKPDIRDAEQAKAYVTKLRSILRYLGTCDGDMEKGNLRADVNVSVRKVGG
                     PLGTRCEIKNMNSINFIGQAIEYEARRQVEILEDGGVIEQETRLFDPNKGETRSMRSK
                     EEAHDYRYFPDPDLLPLEFTQSFVDELKAHLPELPDQKKTRFMTELGLSAYDASVLVA
                     ERESAEFYETVLSRLKNRDRDGKMAANWVINELFGRLNKEGKGIAASPVSAEQLAAIV
                     DLIGEGTISGKIAKDLFEIVWQEGGDPRALVEARGLKQVTDLSAIERVVDDIIAANPD
                     KVAQAKAKPAAIQWFVGQVMKSTAGKADPKAVNALLKDKLGG"
     gene            complement(2941..3234)
                     /locus_tag="VM910_38605"
     CDS             complement(2941..3234)
                     /locus_tag="VM910_38605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68445.1"
                     /translation="MADFKASLECATPASDLAPPLAALWWAAKGDWDAAHKLVQDEDT
                     REAAWVHAYLHRVEGDLGNAGYWYRRAAKPAAKDSLEAEWERIVSAFIEGGKA"
     gene            complement(3241..3532)
                     /locus_tag="VM910_38610"
                     /pseudo
     CDS             complement(3241..3532)
                     /locus_tag="VM910_38610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
BASE COUNT          669 a         1108 c         1022 g          733 t
ORIGIN      
        1 cagatccgac atctgctctg cgacctcgcg gcgcagggcg atcgcgtcac tgcgtccctt
       61 cagagccgcc aggttgaacc atttgtgcgc ggccacgaga ttgatgacgc cggaccgacc
      121 actcgcccaa taaagaccgc gctcgaaaag cacgtcgcaa ctggcggtcg cattgaccgg
      181 caaggcgccg tcgatatcga tgtatccctg aaacatcccc gtctcccctt ttttatgatt
      241 gccgcccgct cgcttgcccg agcgcggtcc gctccaccgt cgaacgcccc actacgtcgg
      301 caacgccgtt tgttcgactg ttgcgacaat gaccgagcaa gcttgaaggg cagcttaagt
      361 atcgcgatga acggcatgta aacgcgagcc ggtgccgcaa gcgcgctatt cggcaaccgt
      421 ggcttttggg gtctggcgtg catcagtcac ctccggttta ccgtccgctt tcgtgggtcg
      481 ataaccatcg ccgacgaacg caaaggcgct gcacgagggg tgtccggaga gaatgcggga
      541 ttggaagagt taaaaaaagc gggatcagcc cgaaaattga aacgagggcg tcggcgaaca
      601 cgtcaggacc agacttcagc tcaggcgaga agccggagtg aattgcggac ccaggaaaag
      661 atccgcaccg aacgaagaaa acccgttttc ttctgctgga ccgaaagggc gatccagccg
      721 cttgacctga tgtctcgccg acaccctcta tcgtcgaccg gagctgaccg gatgaaatcg
      781 cttctttcta gggaagccag ccatcctgcc actccaatga cgcgccggct tcaaagaccg
      841 ccggcgattt agtctggcga gacttacttc ttcttggaga ccttgcgggt cttcttcgca
      901 gtcttcttca ctgcgctctt cgccttcttc ttcgctttct tcgccatatt gccctccgat
      961 aatgagatgg cttagttcgc tgcgtgcatt cgggaatcga aatgcacttc attccgaata
     1021 caccaacgcg tcgaaaaaaa cagggtccca cttaaggaag tgttgatgtg ccgacgtgga
     1081 tgagcacgca tcgcttcgct cgaggatgcg acgtagcaga aacgaaaagc ctgcacgtcg
     1141 tctcgatcag cgaaagtaga tattgcagga agcacctgtg ctgcagccgt ttcccgcacg
     1201 cgtgcggtac tcgatcgatg cacgcaagga gcaagcgtca ggcgccaaca gtggtgagaa
     1261 gtcgctgcat ggactccaac tcttggaatt ttgcaagaaa aatattttca tgattaacgg
     1321 cgctgtgcat cgttgccggc cacagaatcg gttcgttgat gcgaatcgcc gatgtcgatt
     1381 cgcaactagc ttctcgtctc ttgctgacga atttgccgcg cgtcgaaacg aagagaggcg
     1441 agagctgcgc ctcgacagag tgcattcagc cgccgagctt atccttgagc agcgcgttga
     1501 ccgcctttgg atcggccttg ccggcggtcg acttcatgac ctgaccgaca aaccactgga
     1561 tcgctgccgg cttcgccttg gcctgtgcca ccttgtccgg attggccgcg atgatgtcgt
     1621 cgaccaccct ttcgatcgcg gagaggtccg tgacctgctt caggccgcgc gcctcgacca
     1681 gcgcgcgggg gtcgccaccc tcctgccaca cgatctcgaa cagatccttg gcgatcttgc
     1741 cggaaatggt cccttcgccg atcaggtcaa cgatggcggc gagttgttct gctgagaccg
     1801 gcgaggccgc gatgcccttg ccctccttgt tgaggcgacc gaacagctca ttgatcaccc
     1861 aattggcggc catcttgccg tcgcggtcgc ggtttttcag cctggaaagt accgtctcgt
     1921 agaattcggc gctctcgcgc tccgccacca gcacactagc gtcgtaagcc gagagcccaa
     1981 gctcggtcat gaagcgcgtt ttcttctggt ccggcagttc cggcaaatgc gccttcagct
     2041 catcgacgaa gctttgcgtg aactcaagtg gcaagagatc ggggtcgggg aagtagcgat
     2101 agtcgtgggc ctcttccttg gaccgcatcg agcgcgtctc gcctttgttc gggtcgaaca
     2161 agcgggtttc ctgctcgatc accccgccat cctccaggat ttcgacctgg cggcgcgcct
     2221 catactcgat cgcctggccg ataaagttga tcgaattcat gttcttgatc tcgcagcgcg
     2281 tgccgagcgg cccgcccacc ttgcgtacgg agacgttgac gtcggcgcgc aggtttccct
     2341 tctccatgtc gccgtcgcag gtgccgagat agcgcaggat cgaacgcagt ttcgtcacat
     2401 aggccttggc ctgctcggca tcgcgaatgt cgggcttgga gacgatttcc atcagcgcga
     2461 cgcccgatcg gttgaggtcg acatgggaca tggtcgcaga ctggtcgtgc agcaatttgc
     2521 cggcgtcctg ctccaggtgc agtcgctcga taccgacggt cgcggtcttg ccgccgtcaa
     2581 gctcgagcgt gacctcgcct tcacccacga tcggcgactt gtactggctg atttgatatc
     2641 cctgcggcag gtcgggataa aaatagttct tccggtcgaa caccgaacgc aggttgattt
     2701 tcgcgctcag gcccaagccg gtgcgcaccg cctggcgaac gcattcctcg ttgatcacag
     2761 gcagcatgcc cggcatcgcg gcatccacga gcgagacatg actgttcggc tcgccgccga
     2821 aggcggtcga cgctcccgag aacagctttg atttcgaggt gacctgggcg tgcacctcca
     2881 tgccgatcac tacttcccaa tcgccggttg cgcccttgat cagcttgccg ctcgctgccg
     2941 tcatgctttg ccgccctcga tgaaagcgga cacgatccgc tcccattcag cttcgagtga
     3001 atccttcgcc gctggctttg ccgcccggcg gtaccaatag ccggcattgc caagatcgcc
     3061 ctcgacgcga tgcaggtagg cgtgcaccca ggcggcctcg cgcgtgtctt cgtcctgaac
     3121 gagcttgtgt gctgcgtccc agtcgccttt ggcggcccac cacagcgccg cgagcggcgg
     3181 cgcaagatcg gacgcgggcg tcgcgcactc gaggcttgct ttgaagtcgg ctatccccca
     3241 ccaccacctc tcgggagtaa agcggccggc cgcctgctcg atcacttcgc cgagcgagaa
     3301 cagcgtctcc tcgtcgaagg gctgcccgat gagttgcagc gcgagtggca ggccctgcac
     3361 atccttgccg gcgggaacag cgatgccggg aagacccgcc atgttcaccg tcaccgtgaa
     3421 gatgtcgttc agatacattt caacggggtc ggcgccgccc ttctcgccga ggccgaacgc
     3481 ggccgatggc gtggccggcg tcaggatcgc gttcacgcct ttggcaaagc aa
//
LOCUS       DATLJT010001695         5569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_411257,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001695 DATLJT010000000
VERSION     DATLJT010001695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411257"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VM910_38615"
     CDS             <1..263
                     /locus_tag="VM910_38615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68446.1"
                     /translation="KAALDAASLAVALAAHAADVDAALLGYERDRKSAGSQLVARGRY
                     LGAFLEPQSADVPRPPHWHPEIIIREYGAAGVIDDKTTAQAV"
     gene            complement(347..766)
                     /locus_tag="VM910_38620"
     CDS             complement(347..766)
                     /locus_tag="VM910_38620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68447.1"
                     /translation="MCDYSLHNVATRPARIEDKLVTTKFNNSITRGFAAIGEQHVAVC
                     LLPGTEIAFDENVECEPMFGIGILPNKKIGQRLARFRQINMQNAMIHHDALEFPDGQV
                     VLLTDLCEGQAATVLQLPAQPTTEKEAQEQKRVIYVG"
     gene            complement(1431..2978)
                     /locus_tag="VM910_38625"
     CDS             complement(1431..2978)
                     /locus_tag="VM910_38625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HVI68448.1"
                     /translation="MAKRKPKPSSDEISGADIIAFIHQVCLVPEGKYVGQPLKLQRWQ
                     KDFIHQIYDNPHGTRRAILSVGRKNSKTTLSACLLLAHLCGPPAQSKPNSQLFSAAQS
                     RDQAAIIFNLAAKMVRLNPALARIVTIQETAKNLICIELGTRYRALSADASTAYGLSP
                     SFIVHDELGQVRGPRSPLYEALETATGAQENPLSIIISTQSPTDVDLLSVLIDDALAG
                     HDPHTVCKVYTAPTDLDPFDEATIRLANPAFGTFLNADEVMSMAAAARRMPAREAEYR
                     NLILNQRVEPTNAFIAPEVWKTCGTPAGSLGGLTVYGGLDLSEVADLTALVLIGWNGQ
                     KWLVHPTFWLPSEGLVERSTADRVPYDTWRERGFLQVTPGRTVAYQFVAEHLFDLFRR
                     LNIAKIAFDRWNMRHFAPWLLQAGFSEQFVKDHFVEFGQGVASMSPALRDLEQVILEG
                     QLAHGDHPVLNMCANNTVVALDDAGNRKPSKKRSVGRIDGIVALAMAIGVAPLRPPTI
                     DVEALIA"
     gene            complement(2947..3378)
                     /locus_tag="VM910_38630"
     CDS             complement(2947..3378)
                     /locus_tag="VM910_38630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68449.1"
                     /translation="MPRKAAAALAVIPRVPGRGRPEPPPDLDPLEQRIWREVIDALPD
                     HWLDSAGQVLLRRLVAQAAVAERQELRLRQLRAADQDDVELAAAHGAVAKIVAHLLDA
                     LRATPKSQFRARAAGSKAEQAPGWRPWEIRGGGKAQTETEQ"
     gene            complement(4452..4616)
                     /locus_tag="VM910_38635"
     CDS             complement(4452..4616)
                     /locus_tag="VM910_38635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68450.1"
                     /translation="MRKANPNLGDDEFDQKVIEAELDDIVEEFCARWVGPAKKPPAEA
                     CRRTRCAPPR"
     gene            complement(4726..5088)
                     /locus_tag="VM910_38640"
     CDS             complement(4726..5088)
                     /locus_tag="VM910_38640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68451.1"
                     /translation="MSTIVLKGSLFSLLCSLRLYSLLRRGASMEPSNRHPADRLADVR
                     SEIKRLEAEEEQLRAYLLEHPESRTGEAYIAVVGSQSRKRVDLKGLADEVGASIMARF
                     TSFRSAMIVRLQQRQHDA"
     gene            complement(5143..5514)
                     /locus_tag="VM910_38645"
     CDS             complement(5143..5514)
                     /locus_tag="VM910_38645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68452.1"
                     /translation="MVNSDFDLARLRVDLADPRFRRVGKPKKWRREYVQFPWTWIEGL
                     QAAKRVSTYRLALLLVYEHWRMGGRPIVLSNALVIAEGVSRRTKWNALVELEGLGLVR
                     VERRSRKSPRLILCQLPRDKT"
BASE COUNT         1087 a         1740 c         1697 g         1045 t
ORIGIN      
        1 cgaaagcggc actcgatgcc gcgagcctcg cggtcgcact tgctgcacat gcggccgatg
       61 tggacgccgc cctgctcggc tacgagcgag accgcaaatc cgccggttca cagctggtcg
      121 cgcgtggccg ttatctcggc gcgttcctgg agccgcaatc ggcggacgtg ccgcgcccgc
      181 cacattggca tcccgagatc atcatccgcg aatacggcgc ggccggcgtg atcgacgaca
      241 aaacgacagc gcaagcagtt tagctggagc ggcttgcatt ccgatggaat cgtcaaccgc
      301 tccagatgat ttacattgat gggccgttac ggccggacgc tgctcatcaa ccgacgtaga
      361 tgacgcgctt ctgctcctgc gcctcttttt cggtcgtcgg ctgcgccgga agttgcagca
      421 cagtcgccgc ctggccttcg cagagatcgg tcagcagcac gacctgcccg tccgggaatt
      481 ccagcgcatc gtgatggatc atagcgttct gcatgttgat ctgccggaag cgggcgagcc
      541 gctgtccgat tttcttgttg gggaggatcc cgatgccaaa cataggctcg cactcgacat
      601 tctcgtcgaa cgcaatctcc gtgcccggca acagacagac tgcaacatgc tgctccccaa
      661 tcgcggcaaa gccgcgcgtg atcgaattgt tgaacttggt cgtcaccagc ttgtcctcga
      721 tccgggcagg ccgggtggcc acattgtgca aactatagtc acacattgac ggaactcctc
      781 tccctgtgaa ccaggatttc tccggacggc ttccgaagcg acgtgaatgt tagcgaattt
      841 ctgaactgtt cggaagctca ggtctcagac cgctgaaaca tgctggtccg tgcgagtgaa
      901 cccctgatcc gattccgccc ctaggtttcc ggtagcataa cgacacaaac tgcttattcg
      961 atcacctcgt cgacgagcgc gagcagcgtc ggaggcacgt ctcgacggtc gttcctacgg
     1021 tgccggagag gccaacggtt tgcccttttc gaccacaaaa gtcgcgatgg ctttcacggg
     1081 cgacgttccc ttgttgatgc cttcgtggat tttgccaggc tcaatgtaga ttgagttcca
     1141 gccttgtagg tcatcgttgg tttgccttcg tagtctaacg cgaggacccc ttcctggatg
     1201 acaaccagcg cgcctggatg cgtgtgtcgg caaggcgacg ctagccgcgc tgtgcatcac
     1261 aatggccgcc gataagactc cgcccctcga gctgccgccg ttaccgcggc gggaggctga
     1321 actgaagccc ggatcaacga tatttgccgc tgtggtggct gaattccggc gggtcccaat
     1381 gccggtgttg cactgcccga atcacgcgat gcacagtgcc cggacttttc tcaagcaatc
     1441 aaagcctcaa catcgatcgt cgggggccgc aacggggcaa ccccgatcgc catcgccagc
     1501 gccacgatgc catcgatccg gccaaccgaa cgcttcttcg acggtttgcg attgccggca
     1561 tcatcgagcg caacgacggt attgttcgcg cacatattca agacgggatg gtctccatgt
     1621 gcgagctgac cctcaaggat gacttgctcc agatcgcgca acgccggcga catgctcgcg
     1681 acgccttggc cgaactcgac gaagtgatcc ttgacgaact gctcactaaa accggcctgc
     1741 aataaccaag gcgcgaagtg ccgcatgttc cagcgatcaa acgcgatctt tgcgatgttg
     1801 agacgacgga acaggtcgaa cagatgctcg gcgacgaact gatatgccac ggtcctaccc
     1861 ggcgtcactt gtaggaaccc gcgttcacgc caagtgtcgt aaggtaccct atcagcggtt
     1921 gaccgctcga ccagcccctc cgagggcaac caaaatgtcg gatgcaccag ccatttttgg
     1981 ccgttccacc cgatcagaac caacgcggtc aagtccgcga cctcactgag atcgaggccg
     2041 ccgtagaccg tgagcccgcc gagcgagccc gccggcgtgc cgcaagtttt ccagacctcg
     2101 ggggcaatga aggcattcgt cggctcgacc ctctggttaa gaattaaatt gcggtattca
     2161 gcctcgcgcg ccggcattct ccgcgcggcc gcggccatgg acatcacctc gtcggcgttc
     2221 aagaacgtgc cgaacgcagg attggcgaga cggatcgtcg cctcgtcgaa tggatcgaga
     2281 tccgtcggcg cggtgtagac cttgcaaacc gtatgtggat cgtgcccagc aagggcgtca
     2341 tcgatcagca cagagagaag atcaacatcg gtcggtgatt gcgtggaaat gatgatgctc
     2401 aacggattct cttgcgcacc ggtcgcggtt tcaagcgctt cgtaaagcgg ggatcgcgga
     2461 ccacggacct gcccaagctc gtcatgcaca atgaaggacg ggctcaatcc atacgcggtc
     2521 gaggcgtcgg cggagagcgc gcgatagcgc gtcccaagct cgatacagat caggttcttc
     2581 gccgtctcct gaatggtcac gatccgcgcc agcgcggggt taagccgcac catcttggca
     2641 gcgagattga agatgatcgc agcctgatca cgggactgcg ccgcgctaaa caactgcgaa
     2701 ttaggtttgc tctgtgccgg cggaccgcaa aggtgtgcca acagcaagca agcggaaagg
     2761 gtcgtcttgg agtttttgcg tccaacggag aggatcgcgc ggcgggtgcc gtgcgggttg
     2821 tcataaatct ggtggatgaa atccttttgc cagcgctgca atttcagcgg ctgaccgacg
     2881 tacttgccct cggggacgag acagacctgg tgaataaacg cgatgatgtc ggcgccggag
     2941 atctcgtcac tgctcggttt cggtttgcgc tttgccaccg ccgcgaatct cccacggccg
     3001 ccatcctgga gcctgctcgg cctttgagcc ggccgcccgc gcgcggaatt gcgatttcgg
     3061 cgtagcccga agcgcgtcga gcaggtgagc tacgattttt gccacagccc cgtgggcagc
     3121 agcaagctcg acgtcgtctt gatccgcagc gcgcagctgc cgaaggcgaa gctcttggcg
     3181 ctccgccacc gcagcctggg ccacaagccg ccggagcaaa acctgcccag ctgaatcgag
     3241 ccaatggtcg gggagcgcgt cgatcacctc acgccaaatc cgttgctcga gcggatcgag
     3301 atcaggcggc ggttccggac ggcccctgcc cgggacgcgg gggatgacgg cgagagcggc
     3361 ggcggctttg cgcggcatcg gatgattcct ggtgggccgc gcccacgacc aagaccggcg
     3421 cagcgggttt cgtgccgagg aatgttaatt cgatagtgcc ttgaggtcca acctcttcga
     3481 cccggctcag caaccactgt cgcccgccgg cgaacagcgt ctgcacggcg tcatggaggg
     3541 ggccgatcgt cccgccaagt cggagctcgt agacgtcgtc tttcgactcc gtgacggcca
     3601 gcacaatcgt gatggggtcg tccgacatgg cgcagccctc gcgatcctcg gaacgtcgtt
     3661 gccgggaacg acaatattcc ccctccgacc gcccgcaacc cggtccgtca cgggaaacgc
     3721 gccagcaggg aacatcttca gcgcaaacat catccggcgc caccttacca ccggccaaag
     3781 agcaatggcg gtggcgaagc tcgcgcagtt tcgatgcggc gatgtagccg ggcaacgagc
     3841 cgatgcccga aatcagggac cgtcgatttt aaccgtggag gaagccgcaa agctgggtca
     3901 cgtttcacac agcacggtag atcaagcaaa gatcgtatca aagccgtgcg cgcacgcggc
     3961 aatctgcgcc cggcaggcga gcgggggctt tttaatcgca aagcacggcg gtgaacctgg
     4021 gtgggtgacc acgttagcgc agctggccgt ggcgggcgcc gcgaccattg gccctccaga
     4081 gttttggaga ctaccccccc gatggtctgc aacgaccatt agagggctca ggacgcggtg
     4141 gtggcgctga tgcgcaccac gatagtcgga tggcgcgccg gcgcaatagg tggcggttgg
     4201 aagccgcgcg gcggcgcgct tagttcgtgc ggcgcacgga caacgggttc ggcaggccga
     4261 cgacgtgccg gcggttatcg tgcgcgcgtt aacctctcta gctcacgaag cctcgcacta
     4321 accgcggccg cgaacgcaat ggacgcgcgc gcgagttcct cgacctcgtg ccggtgcgcg
     4381 gccaatgccg atgcgatgcg gccgtcaacg tattcgttcc acgccgacac attgcacgtg
     4441 cagcatcgcg ttcagcgcgg cggcgcgcag cgcgtgcgtc ggcacgcttc tgctggcggt
     4501 ttctttgcag gcccaaccca gcgagcgcag aattcctcga cgatgtcatc aagctcggcc
     4561 tcgatcactt tctggtcgaa ctcgtcatcg ccgagattgg gatttgcctt gcgcatggcg
     4621 gcctctaggc ggcgggtatg ggctcgcact cggaatgtcc tgaccccggc cttgagacga
     4681 gcgcgtgaac ggcgggctcg agcggcgtcc ggcgatggcg agcgctcaag catcgtgctg
     4741 ccgctgttgc agccgcacaa tcatggccga tcggaagctg gtgaagcgcg ccatgatcga
     4801 ggcgccaacc tcgtcggcca ggcctttgag gtctacccgc ttgcgggatt gagagccgac
     4861 gacggcgata tacgcctcac cggtccggct ctcaggatgc tcgaggaggt aagcgcgcag
     4921 ctgttcctct tccgcctcaa gccgtttgat ctcagagcgg acgtcggcca ggcgatcagc
     4981 ggggtgacgg ttagacggct ccatcgaagc ccccctacgt aagagagaat acaagcgtag
     5041 agaacacaga agagagaata gggatccctt gagaacgatc gtgctcatgt ttaggcgcca
     5101 tcatgagaac gatcgtgcac atgctcatgg gaacgatcgt gctcatgttt tatcccgtgg
     5161 gagctggcac aggatcaggc gcggggactt ccgggagcgc cgttccaccc ggacgagccc
     5221 gagcccttcc aactcaacca gggcattcca ttttgtccgc cgcgataccc cttcggctat
     5281 cacaagggcg ttcgacagca cgataggccg cccgcccatg cgccagtgct cgtaaaccag
     5341 caacaaggcg agccgataag tgctgacgcg tttcgccgcc tgcaggcctt cgatccacgt
     5401 ccacgggaat tgaacgtact cccgccgcca cttctttggc ttacccacac gacgaaagcg
     5461 tgggtccgcc aagtccactc gaagccgtgc gagatcaaag tcgctgttta ccactatgct
     5521 gccccctgtt tagtcgcctc tcgtgccgtg atcagccgca tgacgcggt
//
LOCUS       DATLJT010001696         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_869269,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001696 DATLJT010000000
VERSION     DATLJT010001696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869269"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="gmd"
                     /locus_tag="VM910_38650"
     CDS             <1..804
                     /gene="gmd"
                     /locus_tag="VM910_38650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVI68453.1"
                     /translation="QSHVQVSFETPEYTANADGLGTLRLLEAIRILGRETKTRFYQAS
                     TSELFGKAQEIPQKETTPFYPRSPYAAAKLYAYWITVNYREAYGMHASNGILFNHESP
                     IRGETFVTRKITRAVASIEHGLQQKLFLGDLNALRDWGHARDYVEGMWMMLQQAEPDD
                     YVLATGEAHSVREFVEKAFAQVGKIIVWQGSGVEEKGIDKSTGRVLVEIDPRYFRLTE
                     VDTLLGDASKAQAKLGWRHKTKFDTLVADMVEADMVAVVEERKRRNRQA"
     gene            972..2054
                     /locus_tag="VM910_38655"
     CDS             972..2054
                     /locus_tag="VM910_38655"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVI68454.1"
                     /translation="MIGVGIVGCNYGRTVLIPAFRQDPRCEVVALAGTDTARTAELAR
                     AANVARGLGSWQAMVEEPAVAVIAVAVPPDLQPTIAQRALDLGKPVFLEKPLAADLAG
                     ARAILESARKSARPTVVDFNFPELPSWRRAKALLDDGAIGRLRNVVVTWNFANRATRL
                     RLDSWKTRGDGGGGLLGNFVSHCFYNLEWLCGPISGLTARLFALPNWHADGSIALALA
                     FASGAGGSLQVSCASFLGSGYRLELYGEDGALVLANPTFDYFRGFELKFARHGDDALK
                     PIAIEDADEERFPDSRVSPVSRLVRRLIDACESGGSPSPGVAEGYRVQCLIDAARRAH
                     ASGRWIDVVPPAAHSPDLVGAGLGKP"
BASE COUNT          382 a          657 c          706 g          416 t
ORIGIN      
        1 cagtcgcatg tgcaagtctc gttcgagacg ccggaatata cggccaacgc cgatggactt
       61 ggaacgctgc gcttgctcga ggccattcgt atcctcggcc gcgaaaccaa gactcgcttc
      121 tatcaagcct cgacctccga gctgttcggc aaggcgcagg agattccgca aaaggaaacg
      181 acgccgttct atcctcgctc gccttacgcg gcggcgaagc tctatgccta ttggatcacg
      241 gtcaattacc gcgaagccta tggcatgcac gcgtcgaacg gtattttgtt caatcacgag
      301 agtccgatcc gcggtgaaac gttcgtcacc cgcaagatca cgcgtgcggt ggcgtcgatc
      361 gaacatggtt tacagcagaa actcttcctc ggagatctca atgccctgcg cgactggggg
      421 catgcgcgcg actatgttga ggggatgtgg atgatgctgc agcaggccga gcctgacgac
      481 tacgtgctcg cgaccggcga ggcccattcg gtgcgtgaat tcgttgaaaa ggccttcgcc
      541 caggtcggaa agatcatcgt ctggcaaggc agtggtgttg aggagaaggg catcgataag
      601 tcgacaggca gggtgctggt cgaaatcgac ccgcgctatt ttcggctgac cgaggtcgac
      661 actctgctgg gagatgcaag caaggcgcag gccaaactcg gttggcgaca caagaccaag
      721 ttcgacacgt tggtggcaga catggtggaa gcggatatgg ttgcggtggt cgaagagcgg
      781 aaacgtcgca accgccaggc ctgaatgtcg cgggcagatt tgtccgatgt cggatagcca
      841 gccgtggcgg ccttccacgt cgatccatct gccgatgcca caacttcaat tcgggaccat
      901 ggcgcttatg ttggtacgcc tacgcgagct tgcggggctg gtccactgcg gcagcctacg
      961 gagacacgac gatgatcgga gtaggcatcg tcggatgtaa ctacggcaga acggtcctca
     1021 tcccggcgtt ccgccaggac ccccgctgcg aggtcgtcgc gctggcggga accgacacgg
     1081 cgcggacggc ggagctcgct cgggccgcaa acgtcgcccg cgggctcggc agctggcagg
     1141 caatggtcga agagccggcc gttgcggtga tcgccgttgc ggtcccgcct gatcttcagc
     1201 ccaccatcgc gcagcgcgcc ctcgatctgg ggaagccggt tttcctggag aagcccctcg
     1261 ccgccgatct cgcaggcgcg cgggcgattc tggaatccgc gcgcaagagc gcgaggccca
     1321 ccgtcgtcga ttttaatttt ccggagttgc cgtcatggcg gcgcgccaag gctctcctgg
     1381 acgatggcgc gatcgggcgc ttgcggaacg tcgtcgtgac ctggaacttc gcaaatcggg
     1441 ctacgcggtt gcgcctcgac agctggaaga cgcgcggcga cggtggcggc gggctgttgg
     1501 gaaacttcgt gtcccattgc ttttataatc tcgaatggct ttgcggcccg atctcgggcc
     1561 ttacggcccg cctgttcgcg ctgcccaact ggcacgcgga cggcagtatc gccctggcgc
     1621 tcgctttcgc gtcgggcgcc ggcggcagct tgcaggtgag ctgcgcgtct ttcctcggct
     1681 ctggttatcg cctcgagctt tatggcgagg acggcgccct cgtcctggct aatcccacct
     1741 tcgattactt tcgcggcttc gagctcaaat tcgcgcgcca cggcgacgac gcgttgaaac
     1801 cgattgcgat cgaggatgcg gacgaggagc gattccctga ttcacgcgtc tctccggtca
     1861 gccgcctggt gcggcgattg attgatgcgt gtgagagtgg cggttcgccc tcgcctggcg
     1921 tggccgaggg atatcgtgtg caatgcctga tcgacgcggc gcgacgcgcg cacgcgagcg
     1981 gccgctggat cgatgtggtg ccgccggcgg cgcattcccc cgatcttgtc ggcgccggcc
     2041 tcggcaagcc gtaacacatg cgagccaaac acccatccag cgctgcccaa tatgtgccgg
     2101 ctctgaccgg catccgggcg ctcgcggcct tattggtgct ggggatgcac accgagcaga
     2161 a
//
LOCUS       DATLJT010001697         8003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3170572,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001697 DATLJT010000000
VERSION     DATLJT010001697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3170572"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VM910_38660"
     CDS             complement(<1..1347)
                     /locus_tag="VM910_38660"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI68455.1"
                     /translation="MNGAAVIAEILRREGTQFLPCYPRNPLIEACAALDIRPILCRQE
                     RVGVGLADGYTRIKRGKRNGVFAAQAGPGIENAFPGVAQAFSENVPLLVIPAGLPLAR
                     QYVRPVFRAADVYRPVTKWSALAHSVQELPDLMRRAYHAMRSGKGGPVLIEIPAEVWE
                     AEYEGELDYAAVQVQRAAPDPDAVKTAVRMLLKAKNPLLLAGQGVLYAQASDRLSALA
                     ELIPAPVVTTNPGKSAIPESHPLALGASTRSRPKMFTEFMAKADLVLAIGSSLTRTPF
                     GPGVPAGKTIIHSTNDAADINKEYRVDHAVVGDAALVLDAVIAEVGRQKGAGGANALA
                     ALKEEVAAAKKGWLAEWGKHLDSEEVPINQYRVIRDLMCTVDRDNVIITHDSGSPREQ
                     LIPFWETTAPGSYMGWGKSTQLGYGLGITMGAKLAAPDKLCVNIMGDAAIGMTGMDI"
     gene            1533..2789
                     /locus_tag="VM910_38665"
     CDS             1533..2789
                     /locus_tag="VM910_38665"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HVI68456.1"
                     /translation="MACGSGLTRGTVSLTHRSIEALRPAETPYRVSDQRCIGLAVRVA
                     PSGIKTWDLAYRIRGSGKVRRVSLGRVADVSLEKARARANELTSAARAGRDLIAEEEE
                     SRAAAASRLTIEKLIELYVRRRVTGRLRTAKEIESRLRRALTPILHLYADDIRRRDIR
                     ELLDAVADQGIEREAEKRRQTVGAMFRWALSQDIVETDATAGLKAYDPGTPRDRVLTV
                     EEIEALWKWLDSGALPSEPADIMKLELLTGARCGEISGLWTEEIDRDEWTWTLPAARS
                     KNKRPRVTPLVGLARQIIEARLSVVQAGPLFTAETGSVLTAAHIGHYLLVRRDKLPID
                     KFTTHDLRRTVATMLAEMGIALDLVAAVVGHEAGGKDTRTLVRHYVRTDLIERKVHVL
                     RSWDERLQNIVAGRESAKVVRLLKTG"
     gene            3281..4066
                     /locus_tag="VM910_38670"
     CDS             3281..4066
                     /locus_tag="VM910_38670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68457.1"
                     /translation="MGHIRLGTLPKTRKWNQVVTLITGGADVDRIAAASADAAEHGLE
                     RASQDEGLAHTFWLLTQIPQAAQQPNFSERLWELGLTGLSSKPTLFEIVAAFTRAVDG
                     HVRKRAKRTDLGEMAQHAASETLASLAGRELPSLFGPTETDVQQALAKLGRSDRFSIV
                     ARDFFSRLTSRSLGYFLSRELSKHVGPNKRFAAIGDHSEFNAALDLHCREASRIIKEF
                     SGGWYGRTLQQERQITKEEARKFAHVAFKKLRAELRKRREANA"
     gene            4059..5828
                     /locus_tag="VM910_38675"
     CDS             4059..5828
                     /locus_tag="VM910_38675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68458.1"
                     /translation="MPERLIICGGAGRTGGDSTLRLALSGRSQNISLKLEDISKKLIR
                     NIPSLLVDLIEVATYVFCADQATSRGGGVQQGMGSDWRRDFRFIIPVRNPHHWNGRKV
                     VEALSSTLSFLSDDNYAFEFEEATNPVAIQNYLQFGDNGVARFKPDEVILFSGGLDSL
                     GGAVEELSKNGNRIALVSHRSSSKIFDNQKRLVAELKQRFPNRLMYVPVLVTRQEPLR
                     VQEYTQRTRSFLYATLACVIARLFENSRIRFFENGIVSINLPISEQVVGARATRTTHP
                     LVLEFFRQFFSVAIGKSIEIENSFIWKTRAEVIRSLVDRGCGELIKYTVSCTRTYEMT
                     KLYTHCGCCSQCLDRRFAVLAANAAGHDPVEMYRVELLAGPREKANDQTMAESYVRTA
                     LELREMSELAFFGRFSGETARVRDGFPSLSADEVGRQVLNLHQRHAQAIGEVLKAGVE
                     KHSEELINRSLSSSSVLMMTVVPGGAPALATIGKRADAIEMWMDEAESESASSSQSGG
                     EFVPDQAAARAHGPSRTGRRKSKPALERARGAIKELYPNGVPGQAVEPNSDLCRRVGE
                     KLKQSGLRDVSDDTILRAAGRRK"
     gene            5965..6186
                     /locus_tag="VM910_38680"
     CDS             5965..6186
                     /locus_tag="VM910_38680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI68459.1"
                     /translation="MVTPLAYTVAQACTVACTGRTALYEAIKSGELRAVKRGRRTLVL
                     ADDLRAWIERLPAIEVKHTEALLRPADDR"
     gene            6176..7075
                     /locus_tag="VM910_38685"
     CDS             6176..7075
                     /locus_tag="VM910_38685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVI68460.1"
                     /translation="MTAEAIAKALGGRKAGGAWMAQCPAHEDRKPSLSIAQGNKGKVL
                     VRCHAGCEQHRVIAALRTRGIWTDPDRRGGKAIGSRSGKTNQPDLCNETRTQAALRIW
                     AATVPASCILVEIYLAARNIVIPAPAALRFHAAIKHPTGQQWPAMVALVTRGTDGAPL
                     AIHRTFLARDGGGKAPIHPQKMMLGPCRGGAVRLANPAEVLMIGEGIETSLAAMLATG
                     HPVWAALSTSGLRALNLPDDVRNVIVLADGDDAGEAAAWECAWRWKREGRRVRIARPP
                     HGLDFNDVLMGRAPASREARHDQ"
     gene            7065..>8003
                     /locus_tag="VM910_38690"
     CDS             7065..>8003
                     /locus_tag="VM910_38690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68461.1"
                     /translation="MTSDTEYLIAAAIEGAEEIHNPLDGLVERAAADPGAPFEVLQRL
                     AALKKDDRAAFEALRAHLKNVGCRVTALDEAIAEESGDPGRRRPTQADILIGLAEAAE
                     LFHSPDGTGFADLDIDAHRETWVIRSKGFRRWLARRYFEATHGAPSSESLQSALNVIE
                     AKAHFDAPERVVHIRVGGLDDRLYLDLGDKAWRAVEISAAGWRVIANPPVRFRRAAGM
                     QPLPAPVAGGSVETLRSFLNVRSDTDFVLVVAWALAVLRTRGPYPVLALSGEQGSAKS
                     TFSAILRALLDPNTAPLRALPREDRDLFIAATNGHLF"
BASE COUNT         1526 a         2395 c         2582 g         1500 t
ORIGIN      
        1 gatgtccatg ccggtcatgc cgatggccgc gtcgcccatg atgttgacgc aaagcttgtc
       61 cggcgccgcg agcttcgcgc ccatggtgat gccgagcccg tagccgagct gggttgactt
      121 gccccacccc atatacgagc cgggcgcggt cgtttcccag aacgggatga gctgttcgcg
      181 cggactgccg gaatcgtggg tgatgatgac gttgtcgcgg tccaccgtgc acatgagatc
      241 gcggatcacg cggtactggt tgatgggcac ttcctcggag tcgagatgtt ttccccattc
      301 cgcgagccat cccttcttgg ccgccgcgac ttcctccttg agcgcggcga gcgcattggc
      361 gccgcccgcc cccttttggc ggccgacctc ggctatgacc gcatcgagca ccagcgccgc
      421 gtcgccgacc acggcgtgat cgacgcggta ctccttgttg atgtcggcgg cgtcgttggt
      481 ggaatggatg atggtcttgc cggccggcac gccgggcccg aacggggtgc gggtgaggct
      541 cgagccgatg gccagcacga gatcggcttt ggccatgaac tcggtgaaca tcttcggccg
      601 cgagcgcgtg gacgccccga gggcgagcgg atggctttcg ggaatggcgc tcttgcccgg
      661 attggtggtg accaccggcg cgggaatgag ctcggccaag gcgctgagcc ggtcgctcgc
      721 ttgcgcatag agcacgccct gcccggcgag cagcagcgga tttttcgcct tgagcagcat
      781 gcgcaccgcg gtcttgaccg catcgggatc gggggccgcg cgctggacct ggaccgcggc
      841 gtaatcaagc tcgccctcgt actcggcctc ccatacctcg gccggaatct cgataaggac
      901 gggcccgccc ttgccgctgc gcatggcgtg gtaggcgcgg cgcatcaggt cggggagctc
      961 ctgcaccgaa tgcgcgagcg cggaccactt ggtgacgggc cggtagacat cggcggcgcg
     1021 gaataccggc cggacatatt gccgcgccaa cggcagcccg gccggaatga ccaacagcgg
     1081 aacgttctcg gaaaaggcct gggcaacgcc gggaaaggcg ttctcgatgc cgggtccggc
     1141 ctgggcggcg aatacgccgt tgcgcttgcc gcgcttgatg cgggtatagc cgtccgccag
     1201 gccgacgccg acgcgctctt gccggcatag aatcggacgg atgtcgagcg cggcgcaggc
     1261 ctcgatcagc gggttgcggg ggtagcaggg gagaaactgc gttccctcgc gcctgaggat
     1321 ttcggcgatg acggcggcac cgttcatggg cgtatcccgc ctttgtatat ctgttcgcag
     1381 tcgcttgctg tcgtacggga ctgtccgttt tgtgtttgta tcacaatggg ttagagtcca
     1441 tggccgttca ggtcggtgcg tcgggtcccg ccgaatgcca gcaaacttgt tgcgacagcg
     1501 ttgcgacgga cggaacaaaa gaggggtagc aaatggcttg tggctcggga ctgacgcggg
     1561 gaacggtatc gctcacgcat cgttcgatcg aggccttgcg gccggcggag actccgtatc
     1621 gggtctccga tcagcgctgc atcgggctgg cggtgcgggt ggcgccaagc ggcatcaaga
     1681 cttgggactt ggcatatcgg attcggggct cgggaaaggt gcggcgggtt tcgctcggcc
     1741 gcgtggccga tgtgagtctg gaaaaagccc gcgcgcgcgc gaacgagttg accagcgcgg
     1801 cacgggcggg ccgcgacctg atcgccgagg aagaagagag ccgagctgcc gcggcttccc
     1861 ggctcaccat cgaaaaactg attgaactgt atgtgcggcg ccgggttacc ggccgcctgc
     1921 ggacggcaaa ggaaatcgag agccgtttgc ggcgggcgct tactccgatc ctgcatctat
     1981 acgccgacga tattcgccgg cgcgacatcc gggaattgct ggacgcggtg gctgaccaag
     2041 ggatcgagcg ggaagccgag aagcgccggc aaactgtcgg cgccatgttc cggtgggcct
     2101 tgtcgcagga catcgtggaa acggacgcca ccgccggttt gaaggcttac gatcctggga
     2161 cgccgcgtga ccgcgtgctg acagtggagg aaatcgaagc gctctggaaa tggctcgatt
     2221 ccggcgccct cccatccgag ccggcggaca tcatgaagct tgagcttttg acgggtgccc
     2281 gttgtggcga aatcagcggg ctctggaccg aggaaattga tcgcgacgaa tggacctgga
     2341 cgctacctgc cgcgcgctcg aaaaacaaac ggccgcgcgt gacaccgctc gttgggctgg
     2401 cacgacaaat cattgaggca cggctttccg tggtccaagc cggaccattg ttcacggccg
     2461 agacaggaag cgttttgact gcggcccata ttggacacta cctgttggtg cgccgcgaca
     2521 agttgccgat cgacaagttc accacgcacg acctgcggcg cacagtggcg accatgctgg
     2581 cggagatggg cattgccctc gatcttgtcg ctgcggtagt cggccacgaa gcgggcggca
     2641 aggacacccg aaccctggtc cgccattacg tccgcactga cctgatcgag cgcaaggtcc
     2701 atgtgttgcg ctcctgggat gagcgattgc agaacatcgt ggctggtcgc gagagcgcca
     2761 aggtcgtccg ccttctgaag accggctgat cggcgggtcg ctccgccgac ttgtgcgacc
     2821 agtacgcgca cgcgcgcacg caatccatac gtgagattgc tgctcccgcg taaggcgacc
     2881 ctttcaaggg gcgctgccgc ttccaccacg gactcccggc acggccatgg aagggcacgc
     2941 attcgcagcg gcccagcgga ggaagtgggc actacgttca gcaccttctc cgtcggaagt
     3001 ggacgcaatt acctttgccc gcagcatcca aaacaaactc ttcaggcata cgataattgc
     3061 ggtccgatct aggggcccca tttcggctgt ggcgacgaag cttcgaaccc tcttccctgc
     3121 gtgcgtttca gggctaacat cccccgcgga ggctaggctc gctgccgaaa cacgcggccc
     3181 cgctgccgct gtgacttctg cgggtgcccc ggcggagggt atcgcgttgc aaaaagctgc
     3241 ggctttgacg gcgctgatcg gttgacctcg ggaaaaaccg atgggccaca ttcgcctcgg
     3301 aacgctaccg aaaacgcgaa agtggaatca ggtcgtcacc ctgatcaccg gcggtgccga
     3361 tgttgaccgg atcgctgctg caagcgccga tgccgccgaa catggcctcg aaagggcgtc
     3421 gcaggatgaa ggcctggcac atacgttttg gctgctcacg caaataccgc aggccgccca
     3481 acagcccaat ttttcagaac gattgtggga actaggcctc accggccttt caagcaagcc
     3541 cacgcttttc gagattgttg cggcattcac ccgtgcggtc gatgggcatg tccgcaagcg
     3601 tgccaaacgc actgaccttg gagagatggc gcagcatgcc gcctccgaaa cgctggcatc
     3661 cttggctggc cgagagcttc cgagcctgtt tgggccgacc gagacggacg tgcaacaggc
     3721 gttggccaag ctcggcaggt ctgatcgatt cagcatcgtc gcgcgcgatt tcttctcgcg
     3781 tctgaccagc cgctcgctcg gttatttcct cagccgcgag ctgtcaaagc acgtcgggcc
     3841 gaataagcga tttgctgcga tcggcgacca tagtgagttc aatgcagcat tggacctcca
     3901 ttgccgtgag gcatcgcgga tcatcaaaga gttttcaggg gggtggtacg gcaggacgct
     3961 ccaacaagaa aggcagatca cgaaggaaga ggcccgaaag tttgcgcatg tagcattcaa
     4021 gaagctccgc gctgagttgc gtaagcggag agaggcaaat gcctgaacgc ctgatcatat
     4081 gtggcggcgc ggggcgtacc ggcggagact caaccttaag gctcgctctc agcggccggt
     4141 cccagaatat ctccctcaag ctcgaagaca tcagcaaaaa actgattcgg aatattccaa
     4201 gtctgctagt cgatctaatc gaggttgcca cctacgtctt ctgtgccgac caggcaacca
     4261 gtcgaggcgg tggagtgcag cagggaatgg gaagtgactg gcgtcgcgac tttcgcttca
     4321 tcattccggt cagaaatccg catcattgga acgggcgcaa ggtagtagaa gcgctttctt
     4381 cgactctctc gtttttgtca gacgacaatt acgcattcga gtttgaggag gcgactaatc
     4441 cggttgcgat tcagaattat ttacagtttg gcgacaatgg cgtcgctaga tttaagcccg
     4501 atgaagtcat cttattttct gggggcctag attccctcgg cggagccgtc gaggaacttt
     4561 ccaagaacgg caatcgcatt gccctggtca gtcatcgatc ctcatcgaag atattcgata
     4621 atcaaaagcg gctcgtcgcg gagctgaagc agcgttttcc gaaccggctc atgtatgttc
     4681 ccgtacttgt gaccagacag gaaccgctcc gggtccaaga atatacccag cgcacacgat
     4741 cctttctcta cgctacgcta gcctgcgtta tcgcccgcct gttcgaaaat agtcggattc
     4801 gattctttga gaatggcatc gtgagcatca atttgccgat ctccgagcag gtcgttgggg
     4861 ctcgcgcaac gcggactact catcccctgg tcttggaatt tttccggcaa tttttcagcg
     4921 tagccatcgg caagtcgatc gagattgaga attcctttat ttggaaaacc agagcggagg
     4981 ttatccggtc gcttgtcgat cgcggctgcg gtgaattgat caaatatacc gtcagttgca
     5041 cgcgaacgta cgagatgacg aaactttaca cccactgcgg ttgctgttcg cagtgcctcg
     5101 atcggcgctt tgccgttctt gctgcaaatg ccgccgggca tgatcccgtc gagatgtaca
     5161 gagtggagct gctcgccggc ccgcgcgaga aggcgaatga ccaaacgatg gctgagtcct
     5221 acgtaagaac cgccttggag cttcgggaaa tgagcgagct tgcgttcttc ggtcggttca
     5281 gtggtgaaac agcccgcgtc cgcgacggct tcccgtcgct atcagccgat gaggttgggc
     5341 ggcaagtcct caacctgcat caacgacatg ctcaggcgat cggggaggtg ctcaaggccg
     5401 gagtcgaaaa acacagtgaa gagctcatca acagaagtct atcatcgtcg tcggtgctga
     5461 tgatgacggt tgtgcccggc ggagcgccgg cgcttgcaac aattggcaag cgggcggatg
     5521 caatcgagat gtggatggac gaagcagagt ccgaaagcgc gtccagcagt cagagcggtg
     5581 gtgaatttgt tccagatcag gcggcggcac gagcacacgg gccttcgcga acaggccgcc
     5641 gcaagagcaa acctgcgctt gaacgcgcgc gcggcgcaat caaagagctt taccccaacg
     5701 gcgttcctgg gcaagccgtg gaaccgaact cggatttatg tcgacgggtc ggcgagaagc
     5761 tcaagcaatc tgggctacgg gacgtttcag acgacaccat tttgagggcc gccggccggc
     5821 gcaaataaag caaatacggc attgcgctat ttgccgtatt tgcggaatgg gaaatcaccc
     5881 gaacaactct attcatttcg ctatcgcccg cgaaaacgcg ggcacggccg ccgggaccat
     5941 cccggctcaa atggcgagat gaaaatggtt acaccgctag cctacaccgt cgcgcaagcg
     6001 tgcacggttg cgtgcaccgg gcgcacggcg ctctatgagg cgatcaagtc gggcgagctg
     6061 cgcgcggtca agcgcggtcg tcgcaccctt gtgctcgccg atgatttgcg cgcctggatc
     6121 gagagacttc ctgcaatcga ggtgaagcac accgaagcgc tcctgagacc cgccgatgac
     6181 cgctgaagcc attgccaagg cgctgggcgg tcgcaaggcg ggcggcgcgt ggatggcaca
     6241 gtgcccggcc cacgaggatc gcaagccaag cttgtcgatc gcgcaaggca ataagggcaa
     6301 agtgctggtg cgttgccatg ccggatgcga gcagcatcgg gtcattgcgg cactacgaac
     6361 acgcggaatt tggaccgatc ccgaccgacg cggcggcaag gcaatcggct cgcgatcagg
     6421 caagaccaat caaccggacc tatgcaacga aacgcgcacg caagcggcgc tgcgcatctg
     6481 ggccgccacg gtgcccgctt cctgcatctt ggtcgaaatc tatctcgctg ctcgcaacat
     6541 cgtcattccc gcacctgcag cgctgcgctt ccacgccgca atcaagcacc caaccggtca
     6601 acaatggccg gcgatggtgg cattggtaac acgggggacc gacggcgcgc cgctggcgat
     6661 ccatcgcaca ttcctggccc gcgacggcgg cggaaaggct ccgatccatc cgcagaagat
     6721 gatgctgggc ccgtgccgtg gcggtgctgt gcgtctcgct aatccggccg aagtactgat
     6781 gatcggcgag ggcatcgaga ccagcctcgc tgccatgctt gcaaccggac atcccgtctg
     6841 ggctgcgctt tcgacttctg gactacgcgc tctcaaccta ccggacgacg tgcgcaacgt
     6901 aattgtgctg gccgacgggg acgacgccgg cgaggcggcg gcatgggagt gcgcttggcg
     6961 gtggaagcgc gaaggacggc gtgtgcgcat cgctcgccct ccgcatggct tggacttcaa
     7021 cgacgtgctg atgggccgcg cgccagcatc gagggaggcg cgccatgacc agtgacactg
     7081 agtacctcat cgccgctgca attgagggtg ccgaggaaat ccataatccg ctagacgggc
     7141 tggtcgagcg cgccgctgcc gatccgggcg cgcccttcga ggtgctccaa cgacttgcgg
     7201 cgctgaagaa ggatgaccgc gctgcgttcg aggcgctgcg ggcgcacctc aagaacgtag
     7261 gctgtcgcgt gaccgcgctc gacgaggcga tcgccgagga gagcggtgat cctggcaggc
     7321 ggcgtccgac gcaagccgac atcttaatcg ggcttgccga ggccgcagag ttgtttcact
     7381 cgccggacgg gaccggcttc gcggatctcg acatcgatgc ccatcgggag acctgggtaa
     7441 tccgcagcaa agggttccgc cgctggctcg cacgtcgcta ctttgaggcg acacatggcg
     7501 cgcccagttc cgaatcgctg caatcggcgc tgaacgtgat cgaggcgaag gcgcatttcg
     7561 atgcgccaga acgagttgtc catatccgcg tcggagggct ggacgaccgg ctctatctcg
     7621 atctcggcga taaggcctgg cgggcggtgg agatcagcgc cgctggttgg cgggtaattg
     7681 cgaacccgcc ggttcgcttc cgccgcgccg ccgggatgca gcctttgcca gcgccggtgg
     7741 ccggcgggtc ggtggagaca ctccgctcct ttctcaacgt ccggtcggat accgatttcg
     7801 tgctggtggt cgcctgggcg ctcgccgtct tgcgcactcg cggcccgtat ccggtgctgg
     7861 cgctgtcggg cgagcagggt tcggcgaagt ccacgttttc cgcgatcctg cgggcgttgc
     7921 tcgacccaaa caccgcgccg ctgcgggcac tgcctcgaga ggaccgcgac cttttcattg
     7981 cggcgactaa cggccatctg ttc
//
LOCUS       DATLJT010001698         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_412429,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001698 DATLJT010000000
VERSION     DATLJT010001698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412429"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2634)
                     /locus_tag="VM910_38695"
     CDS             complement(<1..2634)
                     /locus_tag="VM910_38695"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HVI68462.1"
                     /translation="MGSERQIRSAESDVKPTRLAGRFVAGPHVSEPDRAERILRDWLA
                     ELEPAQSAVIDALLDHSFAKTILLGIVEFSPYLFDLIRADAARLIRLLRCEPEAHLAS
                     LIATTSREVIDAPDEAEAMRLLRRVKSEAALLIALCDIGGVWPVMQVTEALTDLAVAS
                     VQSALRYLLRQETLRGRLAPPDKDRPEHDSGLFVLAMGKMGAGELNYSSDIDLIVFFD
                     PDAPTLARDIEPQTFFVRITQALARLLQQRTGDGYVFRVDLRLRPDPASTQVAMSTEA
                     ALHYYEREGRTWERAAMIKARPCAGDLRAGEAMVSELAPFVWRRHLDFAALADVHDMK
                     RQMQTYRGQREIAVEGHNIKVGRGGIREIEFFAQTQQLIAGGRHVELRVRPTLQALSV
                     LASSDWITYQARDELAAAYQFLRRVEHRLQMIADEQTHSLPDEAEAMERFACFFGYEG
                     RTAFARDLLGHLKIVQRHYGKLFEGDPTGTVKLPDVDYGAGPEDKRLLEHLASLGFKE
                     PAAVAGTVQVWLSGEYRVLRTQSTRSAFAEIVPALIKGLADAEEPDRAVAAFDRFLQA
                     LQRGGRLFTLLGQNRELVALIALVLGAAPRLGDMLARQPQMMDGLIDPRFFGAMPDKR
                     ELSTRLASTLKDADSYEEFLDRLRLFGQESLFLIGTRILSGTVSAQQASTAFADVAEG
                     IVHTVHGLVRDRFAAQHGRIKGQETAIIAMGRLGSREMTASSDLDLILLYDFDGEDPD
                     SDGPKSLQGAHYFARFTQRLISAFTTRTNYGVLYEIDMRLRPSGRAGPVASSISSFAD
                     YQAHEAWTWEHMALTRARVVSASAEFRARIESIICEVLTRRRDPIAIANDVADMRRAI
                     AQEKGESDHWDLKYAAGGMVDI"
BASE COUNT          506 a          910 c          861 g          483 t
ORIGIN      
        1 gatatcgacc atgccgccgg cggcgtattt gagatcccaa tgatcgctct cgcccttctc
       61 ctgcgcgatc gcgcgccgca tgtcggcaac gtcgtttgcg attgcgatcg gatcgcggcg
      121 ccgcgtcagc acctcgcaga tgatgctctc gattctggcg cggaattcgg ctgaagccga
      181 tacgacgcgc gcacgcgtca gtgccatgtg ctcccaggtc caggcctcgt gcgcctggta
      241 gtcggcaaaa gaagagatgc tggaggccac agggcctgcg cgccccgaag ggcgcagccg
      301 catgtcgatc tcgtacagca cgccatagtt ggtacgcgtg gtgaaagcac tgatcaggcg
      361 ctgcgtgaag cgcgcgaaat aatgtgcgcc ctggagcgat tttggtccgt cggagtccgg
      421 atcttcgccg tcgaaatcat agagcaggat caggtcgaga tcggagcttg ccgtcatctc
      481 gcggctgccg agccgaccca tggcaatgat cgcggtctcc tgccccttga tgcggccatg
      541 ctgggcggcg aagcgatccc gcaccagccc atgcacggta tggacgatac cctcggcgac
      601 gtcggcaaag gcggtgctcg cctgttgcgc tgacaccgtg ccggacagga tgcgggtgcc
      661 gatcaggaac aggctctcct gcccgaacag ccgcaggcga tcgaggaact cctcatagga
      721 atccgcatcc ttcagcgttg acgcaaggcg cgtcgagagt tcgcgcttgt ccggcatcgc
      781 gccgaagaag cgcggatcaa tcaggccatc catcatttgc ggctgccgcg ccagcatgtc
      841 gccaagacgc ggggctgcac caagcacgag agcgatgagg gcgacgagct cgcggttctg
      901 accgagcagc gtgaacagcc ggccgccccg ctgcagcgcc tgcaggaagc ggtcgaatgc
      961 ggcgaccgca cgatcgggct cttcggcgtc ggccagtccc ttgatcaggg ccggtacgat
     1021 ttcggcaaag gcgctcctcg tcgattgcgt tctgagcacg cgatattcgc cggaaagcca
     1081 gacctgcacg gtccccgcga ctgcggccgg ctccttgaag ccgagcgatg ccagatgctc
     1141 cagcagtcgt ttgtcctcgg gccccgcacc atagtcgaca tccggcagtt tgacggtgcc
     1201 ggtcggatcc ccctcgaaca gtttgccgta atggcgctgc acgatcttga ggtgaccgag
     1261 caaatccctg gcgaaggcgg ttctgccctc atagccgaag aagcaggcga agcgttccat
     1321 ggcctcggct tcatcgggca ggctgtgggt ctgttcgtcc gcgatcatct gcagccggtg
     1381 ctcgacgcgg cgcagaaatt ggtaagccgc cgcgagttcg tcgcgcgcct gataggtgat
     1441 ccagtcgctg gaggccagca cactcaacgc ctgcaatgtc ggcctcaccc gcaattcgac
     1501 gtggcgcccg ccggcaatca attgctgggt ctgcgcgaaa aactcgattt cgcggatgcc
     1561 gccgcggccg accttgatat tgtgcccctc caccgcgatc tctctctggc cgcgataagt
     1621 ctgcatctgc cgcttcatgt cgtgcacgtc tgcaagcgcc gcaaaatcca gatgcctgcg
     1681 ccagacgaag ggcgcaagtt cggagaccat cgcctcgccc gcgcgcaggt cgccggcgca
     1741 aggccgtgcc ttgatcatgg cggcgcgctc ccaggtgcgc ccttcccgtt cgtaataatg
     1801 cagtgccgct tccgtcgaca tcgcgacctg cgtggaagcc ggatccggcc gcagtctcaa
     1861 gtcgacacgg aacacgtaac cgtcgccggt gcgttgctgc agcagccgcg ccagcgcctg
     1921 ggtgatacgc acgaagaagg tctgcggctc tatgtcgcgc gccagtgtcg gcgcgtccgg
     1981 atcgaagaac acgatcaggt cgatgtcgct ggaatagttc agctcgcccg cgcccatctt
     2041 gcccatcgcg agcacgaaca ggccgctgtc gtgctcaggc ctgtccttgt cgggcggcgc
     2101 aagccgcccg cgcagcgtct cctgtcgcaa cagataacgc aacgccgact gcaccgaggc
     2161 gaccgcgagg tcggtcagcg cctccgtcac ctgcatcacc ggccagacgc cgccgatgtc
     2221 gcaaagcgcg atcaacagcg ccgcctcgga tttcacccgc cgcagcaggc gcatcgcctc
     2281 ggcttcgtcc ggggcgtcga tgacctcacg ggaggtcgtc gcaatgagcg aggcgagatg
     2341 cgcctccggc tcgcagcgca atagccggat cagccgcgcg gcgtcggcgc ggatcaggtc
     2401 gaagagatag ggggaaaact cgacaatccc gagcagaata gtttttgcga aggaatgatc
     2461 gagcagcgcg tcgatgacag ccgattgcgc cggctcgagc tcggcaagcc agtctctcag
     2521 gatccgctcg gccctgtcgg gctcggacac gtgaggtcct gccacaaacc gcccagccaa
     2581 tctggtcggc ttgacgtccg attcagcgga gcggatttga cgttcgctac ccatttcgac
     2641 gcgattcctt catgcgaacg acaaatccaa agctccgctg gcagcgatgt ctgctcgacg
     2701 tccgttgact ctaacattcg caaacccgcc tccccagcaa aatgcgaacg tcgggaccgg
//
LOCUS       DATLJT010001699         8690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1333254,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001699 DATLJT010000000
VERSION     DATLJT010001699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333254"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VM910_38700"
     CDS             <1..974
                     /locus_tag="VM910_38700"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HVI68463.1"
                     /translation="NAACRKAAGMTSPASRQKPRALGQGSHIAMIAPASPANGERIAA
                     GKRELERLGFRLVPQREMSSQGYFAGSAAERRGEFLDALNDPSIDALIATRGGYGSAY
                     LLDEGWPETLASTKPVVGFSDLTTLQVYLWQLHRWPTFYGPMLAAGLDAGADTAYGYD
                     SASFRHALTNSSCSWEASLGGETLHAGSAEGILLGGAMTLLEATLGTPWEIDTAGSIL
                     ILEDRGMKPYQVDRMLLHLKHAGKFEGVRAMILGDFPECEPPVPNSPSVRDVAKRILA
                     PLHVPVLCGAPVGHTARPMLTLPLGVPARLTAEGQGTLKILEPTVRP"
     gene            971..2434
                     /gene="mpl"
                     /locus_tag="VM910_38705"
     CDS             971..2434
                     /gene="mpl"
                     /locus_tag="VM910_38705"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HVI68464.1"
                     /translation="MTPSDSAKQKHVHVIGIAGSAMAPLAGMLREHGFRVTGSDAGVY
                     PPASTLLDSLGIAYNHSFDARNLTPGPGLVVVGNIIARGNPELEETLERKIPYRSMPE
                     ILEEVFLPGKHSIVVSGTHGKTTTTAMLAWVFHSAGKRPTFLVGGVAENFGKSYGLGN
                     GAEMILEGDEYETAFWDRGPKFFHYHPDDLIITALEYDHADIYSDFAVYQLAFKRLVN
                     LVPRNGRVVIWGDTKDSGPALRQAAANAFCPIVTYGFSSENDWVASEPAFSGDHMKFR
                     VTRQGQRFGEFSLAASGRHNVLNALAAIIVAEGRGVSAASLEQSLASFRSVRRRLDVK
                     GEHHGVLVVDDFAHHPTAIRATIEAARGRWPNRRLWAILEPRSNSMRRKVFQESLPQA
                     LALADCVALGGVFRAQQLGEENRLDPETVAASVRTLGKEADVVSGGEAMADFLVANAK
                     ANDLLLIMSNGSFDGLCEKLLAKLDAALPVPAGANPG"
     gene            2431..4368
                     /locus_tag="VM910_38710"
     CDS             2431..4368
                     /locus_tag="VM910_38710"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVI68465.1"
                     /translation="MNFFPRGTKTQNFASRARRSGRAVRFFLAREAALSAFGSRRLRL
                     PTPRQKSVELVVFLLTATLCGLMAQPARATIQYQVSLAKPSEHLFHVTVTVPDVHGPL
                     VLQMPAWNALYQVRDFSSRVQQVSAESNGKELAVEKLNKLTWRARAEGTVSVRYAVFW
                     DSPGPFASQLNDEHAFINPAMVFLDVPNRRSESVAVLLQDLPKEWEVATPLDHVRTQS
                     GANVVDTVSAAGYDALCDGPMEVGTFRTFQIEGGPHPIAVAIHGDNWKQADVEAVLKK
                     ICSYQVQLMDGAPFLRYLFIFHIGKAAQGAGGGMEHANGTAINVSSGQALAGVAAHEF
                     FHLWNVKRIRPASLEPIDDTREQYTRALWFAEGVTSSYGSYTLVRSGIWSKQDFYGDL
                     AQNINELESRPANRWQSAEQASLDAWLEKYSYYNGPDFSVSYYSKGQILGLLLDILIR
                     DRTGNAKSLDDVMRKMNDDFARKGRFYRDSLDVRLSAEAVAGGSFADFFQDHVARAEP
                     LPYADILGKAGLLLEKQEIQRPDLGFALERESGAKATVRSVISGSAADRAGLRAGDEI
                     ETWNGENVPRRVEGWLRSRKPGDILRLQVRRNEQPSDISFALGGRTEEIFVVSEDANA
                     TAQAKAIRAGLLGGGAAAAAN"
     gene            4464..5195
                     /locus_tag="VM910_38715"
     CDS             4464..5195
                     /locus_tag="VM910_38715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVI68466.1"
                     /translation="MLRTLFVAIALSLYIIIVGIPLLIFTVLARNPDALYVAGARGTL
                     FLMRAVGVRVRVKGTERIPPGTCLFVANHTSSADAPAVVNAIPCRIAILLKKSLFQWP
                     IVGQAFTLAHFIPVERSRHESAIASLEKAISAMREGQSFLIYPEGTRSPDGRLQQFKK
                     GAVVMAIKAGVPIVPVACSGAHRVMEKRSLVIHPGEIVVEFLDAVDASRYTFEQREAL
                     NDVVHDAMAAGLPPDQRPIGFPGAA"
     gene            complement(5216..6253)
                     /gene="lpxD"
                     /locus_tag="VM910_38720"
     CDS             complement(5216..6253)
                     /gene="lpxD"
                     /locus_tag="VM910_38720"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVI68467.1"
                     /translation="MKLADLALKLSCRLEGDPAEEITAVAGMDNALPGNVTFLANRRY
                     FPLLHTTRASAIFIEDGIRIDREPGLLPLAALRTANPYLAFAQAIELFYRPPAYPPGI
                     HPTAVIAQSAKIGSGAHIGPYCFVDEEVALGASAVLHSFVTLYRGAQIGDNFFAHAHA
                     VVRERCRIGNRVILQNGVIIGGDGLGFAKQKDGSWYKMAQSGPAVLEDDVEVQSNACV
                     DRATVGETRIGRGAKLDDLVLIGHASQVGANAMLCGQVGLAGSTRIGDGSILAGQVGT
                     AGHLTIGKGAVITAKSGVPNDVPSAALYSGYPAVENRQWLKTQALLNRLPELQRKVRE
                     LEEQIEKLKSR"
     gene            6379..7371
                     /locus_tag="VM910_38725"
     CDS             6379..7371
                     /locus_tag="VM910_38725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HVI68468.1"
                     /translation="MLAAVLRDAKRIVEKPLEVGEAERPEVRARQVLLRVKACGVCRT
                     DLHIVEGELAAQRRPVIPGHQIVGEIVDGASPALPIGTRVGVSWMGGVDGTCPLCRRG
                     MENLCDAPVFTGYSVDGGYADYAAARTDFVFPLPGELSDTQAAPLLCAGIIGFRSIRV
                     AGVERGERVGLFGFGASAHLAIAVLRAWGCGVYVSTRGESHRRLAESLGASWVGSERE
                     KPPVELDRAITFAPSGDVVVAALGSLRKGGVVAINAIHLDRMPEFDYDKLLWGERQIR
                     SVANMTRQDARDFIAVAAEIELKPKVTLFPLEQANEALAALAADAIDGAAVLVP"
     gene            complement(7776..8648)
                     /locus_tag="VM910_38730"
     CDS             complement(7776..8648)
                     /locus_tag="VM910_38730"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVI68469.1"
                     /translation="MVVSCEITDKQNRQRPGRVDGANRSVIDRSAVEENRAFAPETIP
                     AAPGWPPVDRESSRVGRNSLDPAERCSLAGSAGEISPSFDVLAASAGLGGAGRLAENL
                     ARIPERVEPTPATGVERVVPGWQFCSGEKRGAEVGKTKRGKGTKWMVVVDGRGLPLGN
                     SLHSASPSEMRLAEQTLASIRVSRRHQAGRPRQKPLRVIADKGYDCDALRRRLRKRGI
                     QLIAPLRLNRRRTPPQDGRVLRRYKRRWMVERTFAWLGNFRRLVVRYDRSLTIYRAFF
                     HIACFMIVLRRVVQ"
BASE COUNT         1637 a         2736 c         2664 g         1653 t
ORIGIN      
        1 gaaatgctgc ctgtagaaag gcagcaggaa tgacctcgcc cgcctccagg caaaaaccaa
       61 gggctcttgg ccagggttcg cacatcgcta tgatcgctcc cgcctcgccc gccaacggcg
      121 agcgcatcgc cgcaggcaaa agggaactcg agcgcctcgg tttccgcctg gtgccgcaac
      181 gcgagatgag ctcgcagggc tacttcgcgg gctcggccgc agagcgccgc ggcgaatttc
      241 tcgatgctct aaacgaccct tcaatcgacg ctctgatcgc cacccgcggc ggttacggat
      301 cggcttatct gctggacgaa ggctggcctg agaccctcgc ttcgacgaaa ccggtcgtcg
      361 gcttcagcga cctcacaacg ctccaggtct acctctggca gctacaccgc tggccgacct
      421 tctacggtcc catgctcgcc gcggggctcg atgccggagc cgatactgcc tacggctacg
      481 actccgcatc ctttcgccat gccctcacga actcctcttg cagctgggaa gctagtctcg
      541 gaggcgaaac acttcacgcg ggctcggcag aaggaatcct cctgggcggg gccatgaccc
      601 ttctcgaagc cacgctcggt acgccctggg agatcgacac cgccggctcc atcctgatcc
      661 tcgaggatcg cgggatgaag ccgtatcagg tcgaccgcat gctcctgcac ctcaagcacg
      721 caggcaaatt cgaaggcgtc cgcgccatga ttctcggcga tttcccggaa tgcgaacccc
      781 cggttccgaa tagcccttcg gttcgagacg tggcaaaacg gattctcgcg cccctacacg
      841 tccccgtcct ttgcggcgcg ccggtcgggc ataccgcgcg gcccatgctg acattgcctc
      901 ttggtgtacc cgcccgcctc actgccgaag gccaaggtac actgaagatc ctggagccaa
      961 ccgtccgtcc atgacaccct ccgattctgc aaagcaaaaa cacgtccacg tcatcggcat
     1021 cgccggatcg gccatggcgc cccttgccgg aatgctgcga gaacacggct tccgcgttac
     1081 cggatcggac gccggcgtct atccgcccgc ctcgacgctg ctcgactcgc tcggtatcgc
     1141 ctacaaccac tccttcgatg ccaggaacct cacgcccggg cccggcttgg tggtcgtcgg
     1201 caacatcatc gcccgcggca atcccgagct ggaagaaact ctggagcgta agattccgta
     1261 ccgctccatg cccgaaatcc ttgaagaagt ctttcttccc ggcaaacact ccatcgtcgt
     1321 aagcggcacg cacggcaaga ccacgactac cgccatgctg gcctgggttt ttcattcggc
     1381 cggcaagcgg ccgacttttc tggtgggcgg cgtcgcggaa aatttcggca agagctacgg
     1441 cctcggcaac ggcgcggaaa tgattctcga gggcgacgag tacgaaaccg ccttttggga
     1501 ccgcggcccc aaattttttc actatcaccc cgacgacctc atcatcaccg cgcttgaata
     1561 cgatcatgcc gacatttact cggacttcgc cgtgtaccaa ctggcgttca agcgcctggt
     1621 gaatctcgtt ccgcgcaatg gccgcgtggt catctggggc gacacgaagg attcaggccc
     1681 cgcgcttcgc caggcggccg ccaacgcgtt ttgcccgatc gtcacctacg gcttttcgtc
     1741 ggaaaacgac tgggttgcca gcgagccggc tttctcgggc gaccacatga agttccgcgt
     1801 cacccgccag ggccaacggt tcggagagtt ctctctcgcc gcttcgggtc gccacaacgt
     1861 tttgaacgcg ctcgccgcca tcatcgtcgc tgagggccga ggcgtaagcg cggcgtctct
     1921 cgagcaatct ctcgcgtctt tccgctccgt tcgccgccgc ctcgacgtca aaggcgagca
     1981 ccacggcgtc ctggtggtag atgacttcgc ccaccatccc accgcgattc gcgccacgat
     2041 cgaagcggcc cgcggccgct ggccgaaccg ccgtctctgg gctattctgg agccgcgttc
     2101 gaactctatg cgccgcaaag tttttcagga atcccttccg caggctctcg cgctcgccga
     2161 ttgcgtcgcg ctcggcggcg ttttccgcgc acagcagctc ggcgaggaaa accgcctcga
     2221 tcccgaaacg gtcgcggctt cggttcgcac actcggcaaa gaggccgacg tggtctccgg
     2281 cggagaagcc atggccgatt ttctcgtggc caacgccaag gccaacgatc tcttgctcat
     2341 catgtccaac ggcagcttcg acggcctgtg cgagaagctc ctcgccaaac tagacgccgc
     2401 actgccggtc cccgcaggag caaacccagg gtgaactttt tcccgcgcgg cacgaaaacg
     2461 caaaacttcg catcccgagc ccgccgcagc gggcgtgcgg tccgcttctt ccttgcgcgg
     2521 gaggccgccc tctccgcctt tggctctcgc cggttgcgcc tgccaacccc gcgtcaaaag
     2581 agtgtggagc ttgtggtttt cctgctcacc gccactcttt gcggcctcat ggcccaaccc
     2641 gccagggcca cgattcaata ccaggtctcg ctcgcgaaac catccgagca tctgtttcac
     2701 gtgacggtga cggtccccga cgttcacggc ccgctggtgc tacagatgcc ggcgtggaac
     2761 gcgctgtacc aggtccgcga tttcagcagc cgcgtccagc aagtaagcgc cgaatcgaac
     2821 ggcaaagagc tggccgtcga aaagttgaac aaactcacct ggcgcgcgcg cgccgaaggc
     2881 accgtcagcg ttcgctatgc agtcttctgg gactcacccg gccccttcgc ctcgcaactg
     2941 aacgacgaac acgccttcat caatccggcc atggtctttt tggatgttcc caatcgccgt
     3001 tccgaatcgg tggccgtgct ccttcaggac ctgccaaagg agtgggaggt cgccacgccg
     3061 cttgaccacg tgagaacaca aagcggggcc aacgtcgtcg acaccgtttc cgccgccggc
     3121 tacgacgcct tgtgcgatgg gcccatggag gttggcacgt tcagaacgtt tcagatcgag
     3181 ggcggccccc atcccatcgc cgtcgccatc cacggcgaca actggaagca agccgatgtc
     3241 gaagcggtgc tcaaaaaaat ctgctcgtac caagtgcaat tgatggacgg cgcgcccttc
     3301 ctccgctacc tgttcatctt ccacatcggc aaagcggctc agggcgcggg cggaggaatg
     3361 gaacacgcca acggcacggc gatcaacgtc tcctcgggcc aagccctggc cggcgtcgcc
     3421 gcgcatgagt ttttccatct ctggaacgtg aagcgcatcc ggcccgcctc gctcgaaccg
     3481 attgacgaca cgcgcgaaca gtacacgcgc gccctgtggt tcgctgaagg cgtgaccagc
     3541 agctatggca gctacacgct cgtgcgctcc ggcatctggt ccaagcagga tttctatggc
     3601 gacctcgcac agaacatcaa tgaactcgag tcgcgcccgg ccaatcgctg gcaaagcgcc
     3661 gaacaagcga gcctcgatgc ctggctcgag aaatactcct actacaacgg accggacttc
     3721 agcgtgtcct attacagcaa ggggcagatc ctgggcctct tgctggacat tctgattcgc
     3781 gaccgcaccg gcaacgccaa gtcgctcgac gatgtgatgc gcaagatgaa cgacgacttc
     3841 gcgcgcaaag gaaggttcta tcgcgactca ctcgacgtgc gtctgtccgc cgaagcggtc
     3901 gctggcgggt ccttcgcgga tttctttcaa gaccatgtcg cccgcgcaga gccccttccc
     3961 tatgccgaca ttctcggcaa ggccggcttg ctgctggaaa agcaggagat ccagcggccc
     4021 gatctgggtt tcgcgctcga gcgcgagtcc ggcgccaaag ccacggttcg ctcggtaatt
     4081 tccggttcgg ccgcggaccg cgccggcctg cgcgccggcg acgagatcga aacctggaac
     4141 ggcgaaaacg ttcctcgccg agtcgaaggc tggctgcgta gccgcaagcc gggggacatt
     4201 ttgcgcctgc aagtgcgccg caacgaacaa ccctccgata tctccttcgc gctgggcgga
     4261 aggaccgagg agatcttcgt ggtcagcgag gatgcaaacg ccactgcgca ggccaaggcc
     4321 attcgcgccg gactgcttgg cggcggcgcg gccgcagccg cgaactgact ttcgcgccgc
     4381 accacccgcc ttgcgaatcg ccttgcgcac tgatggcttg acgggcctcc gggctttcga
     4441 gagagtggca ttagcctctg aacatgctgc gcaccctctt cgtcgccata gctctttcgc
     4501 tgtacatcat catcgtcggc attccgctgc ttattttcac cgtgctggcg aggaatcccg
     4561 acgcgctcta tgtggccggc gcgcgcggca cgctctttct gatgcgcgcc gtcggcgtgc
     4621 gcgtgcgtgt caaaggcacg gagcgcattc cgccgggcac ctgtcttttt gtggcgaacc
     4681 ataccagctc ggccgacgcg ccggccgtag tgaacgcgat tccgtgccgt atcgcgattc
     4741 tgctgaagaa gtcgctcttc caatggccca tcgtggggca ggccttcacc ctcgcgcatt
     4801 tcattccggt cgaacgctcg cggcacgaat cggcgattgc cagcctggaa aaggccatca
     4861 gtgccatgcg tgaagggcaa tcgtttttga tctatcccga gggcacgcgc agcccggacg
     4921 gccgcttgca gcagttcaaa aagggcgcgg tggtgatggc catcaaggcc ggtgttccca
     4981 tcgttcccgt cgcgtgttcg ggcgctcatc gggtcatgga aaaacggtcg ctggtgattc
     5041 atcctggcga gattgtcgtg gaatttctcg atgcggtcga tgcttcgcgt tacacgtttg
     5101 aacagcgcga agcgctgaac gacgtggtgc acgacgccat ggcggcggga ttgcctcccg
     5161 atcagcggcc gatcggattt cccggcgcgg cgtagacgaa aggccgccca caagctcagc
     5221 gggactttag tttttcgatt tgctcctcga gttcgcggac cttgcgctgc agttccggca
     5281 gacgattcag cagggcttgg gtcttgagcc attgacgatt ttccacggcc gggtagcccg
     5341 aatagagcgc cgcggaaggt acgtcgttgg ggactccgct tttcgcagtg atgacggcgc
     5401 ccttgccgat cgtgagatgg ccggccgtgc ctacctggcc tgccaggatc gagccgtcgc
     5461 cgattcttgt ggagccggcg agaccgacct ggccgcaaag cattgcgttg gctcctacct
     5521 gcgaggcgtg gcctatcagc accagatcat caagctttgc gccgcggcca atgcgggttt
     5581 cgccgaccgt ggcgcgatcg acgcaagcgt ttgattgcac ttccacatcg tcctccaaga
     5641 ccgccggtcc tgattgcgcc atcttgtacc aggatccgtc tttttgcttg gcgaagccca
     5701 gcccgtctcc accaatgatc acgccgttct ggagaatgac tcggttgcca atacggcagc
     5761 gttcacggac cacggcgtgc gcgtgcgcaa agaagttgtc gccgatttgc gctccgcgat
     5821 agagcgtgac gaagctgtgc agcacggcgc tcgcgccgag cgcgacctcc tcgtccacga
     5881 aacaataagg cccgatatgg gcgcccgagc cgatcttcgc ggattgggca atcacggcag
     5941 tcgggtgaat gcccggcggg taggctggcg gccggtagaa gagttcgatg gcctgggcga
     6001 aggcgaggta aggattcgcg gtgcggagcg cagccagcgg aagcagacca ggctcgcggt
     6061 cgatgcggat gccgtcttcg atgaaaatcg cgctagcccg cgtggtgtgc aggagaggaa
     6121 agtagcggcg gttggcgaga aaggtgacgt tgcccggtag ggcgttgtcc atgcctgcaa
     6181 cggccgtgat ttcctcggcc ggatcgcctt ccaggcggca ggagagcttc agcgcgagat
     6241 cagcgagttt cattttggca gctccagagg aacggagcga gtatagagga agcggctggt
     6301 cgacgcgaag cagcacggag cgaacgggaa tcgacgagca tcgaagaacc gggaagacct
     6361 tccctaaacg gaggtgccat gctggcggca gtgctaagag atgcgaaacg gatcgtggaa
     6421 aaaccgctcg aagtcggtga agcggagcgg ccagaggtgc gcgctcggca ggtgctgctg
     6481 cgcgtgaagg cttgcggggt gtgccggacc gatttacata tcgtcgaagg agaattggcg
     6541 gcccagaggc ggccggtgat tccggggcac cagattgtgg gagagattgt cgacggtgcc
     6601 agccccgcgt tgccgatcgg aacccgggtg ggagtttcct ggatgggcgg ggtggacggg
     6661 acttgccccc tttgccggcg cgggatggag aatctttgtg atgcgccggt ctttaccggg
     6721 tactcggtcg atggcgggta tgcggactac gcggcggctc gcacggactt tgtgtttccc
     6781 ctgcccggcg agctaagcga tacacaggcg gcgcccctgc tgtgtgcggg catcatcgga
     6841 tttcgcagca ttcgcgtggc gggggtcgag cgcggagagc gcgtggggct gtttggattt
     6901 ggggcttcag cgcatctggc gattgcagtg ctacgcgctt ggggttgcgg ggtttatgtt
     6961 tcgacgcggg gcgaatcgca tcggcggctg gcagagtcgc ttggagcgag ctgggttgga
     7021 agcgaaagag aaaaaccgcc ggtcgagttg gaccgggcca ttacatttgc gccgagcgga
     7081 gacgtggtcg tggccgcgct cggcagtttg cgaaagggcg gcgtggtggc gatcaacgcg
     7141 attcacctgg atcgaatgcc ggaattcgat tacgacaaac tgctgtgggg agagcggcag
     7201 attcgcagcg tggccaacat gactcggcag gatgcgcgag atttcattgc ggtcgcggcg
     7261 gagatcgaac tgaagccgaa agtcacgttg tttccgctcg aacaggcgaa tgaggcgctg
     7321 gccgcgctgg ccgcggatgc gattgacggg gcggcggtgc tggtgccgta gggcgaagaa
     7381 cgcaaaaacc ggaagtttct actcacagcg ccgccctacg gaggattatt ccgagcgtcg
     7441 acagcagaat tggcgaagag atcaagtgga atgcgccgat gttcttttag gccgggccca
     7501 tgaggccatc gccatccgaa ggaataccga aggtatatcg tggtgttcaa tctgtttcgg
     7561 caggattgca tccgattggt atttccaagc ggagccaagg tgaaagattc gtccggactg
     7621 ctggaaggcg acggtgccga cagaaggcgt ctggcaatgt ttttcgggcg cgaaggacgt
     7681 gagttccaac gagaaggcgt tgcgggacgt gattgccgga tggctcgaac tattgcggaa
     7741 atagcggttc cggaatactc ggaaaagcct agaagctatt gcacaaccct ccgtaagaca
     7801 atcatgaagc aggcgatatg aaaaaaggct cgatagatcg taagcgaacg atcgtagcga
     7861 accaccagac ggcggaaatt ccccaaccag gcgaacgtcc gctcgaccat ccagcgccgt
     7921 ttgtagcgtc gaagaacgcg accatcttgg ggcggcgtcc ggcgccgatt caagcggaga
     7981 ggagcgatca gctggatgcc ccgctttctc agtctgcgtc gcagcgcgtc acagtcatat
     8041 cctttgtcgg caatcacccg caggggtttc tgccggggac gccccgcctg atgtcgtcga
     8101 ctcacgcgga tcgacgcgag cgtctgctcg gcgagtcgca tttccgacgg ggaggcagag
     8161 tgaagggagt ttcccagagg aagaccgcgg ccgtcgacca ccaccatcca cttcgtcccc
     8221 ttgccccgct tggtttttcc gacttcagcg cccctttttt cgccggagca aaactgccat
     8281 ccaggaacga ctcgctccac tccagttgcc ggcgttggtt caactcgctc aggaatgcgc
     8341 gccaaatttt cagccagacg ccctgctcct cccaatcccg cagacgccgc cagcacgtcg
     8401 aaggatgggg atatttctcc ggcagatcct gccagcgagc accgctccgc aggatccaga
     8461 gaattccttc caacacgcga cgattctcta tccacgggcg gccaccctgg cgctgccggg
     8521 atggtttcgg gagcaaaggc gcgattttct tccactgcgc ttcggtcaat aacggaccgg
     8581 ttcgcgccat cgaccctccc tgggcgctgc cggttctgtt tatccgttat ttcacaagac
     8641 acaaccaccc tgatctcaaa gggttgtatt aggttatgca acagcttcta
//
LOCUS       DATLJT010001700         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4547012,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001700 DATLJT010000000
VERSION     DATLJT010001700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4547012"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2320
                     /locus_tag="VM910_38735"
     CDS             <1..2320
                     /locus_tag="VM910_38735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVI68470.1"
                     /translation="IMRFGIGQPVTRKEDARFLTGRGRYVADLDFARQAHAVFIYSPH
                     AHARIRAIEKATAEQSPGVFAVLTGEDWRADGLGTLDPEVMAEDMGGPKGYRTKRPPL
                     AIDRVRHVGERVAVVIAGTEAQARDAAELVSVDYDVLPAVVHAEDAIGPEAPRLHEGA
                     ANNTSFTMRMGNVDAIDAAFARAHHITRLSLYNNRITAVTLEPRGCIAEYDTGTRRYT
                     LYSSTQNVHGVRHILAHQILHVPESRIRVVARDVGGGFGMKGNVHAEEAVVTWAARRV
                     GRPVKWISSRAEALLGDAQGRDQNVSAELALDADGRFLGLRWTGSHNVGAYIEGAGCI
                     PILLSLQLAPNAYDIPAVAVTSSLVFTNTAPTVPYRGAGRPEAVYIIERLVDQAAREM
                     RIDPDELRKRNLIRPDAFPYQTRTGWTYDTGDYAAALAKCQALADWQGYAARRARSEA
                     AGRRRGRGIVYYVENTGIFNERMELRFDPSGELTIVAGTLSHGQGHETSYAQMVADWL
                     GVPEDKIHLAQADTDEVAIGRGTYASRSMIMGGSALRAAADEIIERGKRFAAHFMEAD
                     AADIAFADGSFMIAGTDRSMPILQVARMSFIPVGLPSELGVGLQGAGAFSPGLPSFAN
                     GCHICEVEVDPETGEVALDRYTVVDDIGTVINPLLATGQIQGGAAQGVGQALIEDIVY
                     GRDNGQLLTGSLLDYGIPRADMLPAIAVEFSPVPSTTNPLGVKGVGEGGTIAATPTVI
                     NAILDALAPLGVLDIPMPATPERIWRAIRERA"
BASE COUNT          441 a          836 c          804 g          439 t
ORIGIN      
        1 catcatgcgc tttggcattg gacaaccggt tacgcgcaag gaggatgctc gcttcctgac
       61 agggcgcggg cgttacgtcg cggacctcga cttcgctcgc caggcgcacg cggtctttat
      121 ctactcgccg cacgcccatg cacggatccg cgctatcgag aaggccacgg cggaacaaag
      181 tccgggcgtt tttgccgtgc tgaccggcga ggactggcgc gcagacgggc tcggcacgct
      241 cgatcccgag gtgatggccg aggacatggg cggaccaaag ggctaccgca ccaagcgtcc
      301 gccgctggcg atcgatcgtg tgcgccatgt cggcgagcgc gttgcggttg tgatcgccgg
      361 aaccgaagcg caggcgcgcg atgcggcgga attggtctcg gtcgattacg acgtgctgcc
      421 cgcggtggtg cacgccgagg atgcgatcgg ccccgaggcg ccacggctcc atgagggggc
      481 tgccaacaac acctccttca caatgcgcat gggcaatgtc gatgcgatcg acgccgcctt
      541 tgcccgggcg catcacatta cgaggctctc gctctacaac aaccgcatca ccgccgtcac
      601 gttggagccg cgcggctgca ttgccgagta cgataccggc acccgccgct acactctcta
      661 tagcagcaca cagaacgtgc acggtgtgcg ccatatcttg gcgcatcaaa tcctgcatgt
      721 cccggaaagc cgcatccgtg tcgtcgcccg cgacgtcggc ggcggctttg gcatgaaggg
      781 caacgtccat gccgaagagg cggtcgtcac ctgggcggcc cgccgcgtcg gccgtccggt
      841 caaatggatc tcatcgcgcg ccgaggcgct gctcggcgac gctcaggggc gtgaccagaa
      901 tgtcagcgcc gagctggcgc tcgatgccga cggcaggttt ctggggttgc gctggaccgg
      961 ctcgcacaat gtcggtgcct atatcgaagg agccgggtgc atcccgatcc tgctgtcgct
     1021 gcagctcgcc ccgaacgcct acgacattcc cgctgtggcg gtcacgagca gcctcgtgtt
     1081 caccaatacc gcgccgaccg tgccctatcg cggcgccggc cgccccgagg ccgtctacat
     1141 tatcgagcgg ctcgtcgacc aggcagcgcg cgagatgcgc atcgatccgg acgagctgcg
     1201 gaagagaaac ctgatcaggc ccgacgcctt cccctatcaa acgcgcaccg gctggaccta
     1261 tgacaccggg gattacgccg cggcattggc caaatgccag gcactggcgg actggcaagg
     1321 ctacgccgcg cgccgtgctc ggagcgaagc tgcgggcaga cgccgcggcc gcggcatcgt
     1381 ttattacgtc gagaacactg gtatcttcaa tgagcgcatg gaattgcgct tcgatcccag
     1441 tggcgaattg acaattgtcg ccggcacctt gtcgcatgga caagggcacg aaacgagcta
     1501 tgcacagatg gtcgccgact ggctcggcgt gcccgaggac aaaatccacc tggcgcaggc
     1561 cgacaccgac gaggtggcga tcggccgcgg tacttatgct tcgcgcagca tgatcatggg
     1621 cggcagtgcg ctacgcgccg ccgccgacga gatcatcgag cgcggcaagc gcttcgctgc
     1681 ccatttcatg gaggcggacg cggccgacat cgcctttgcc gacggcagtt tcatgatcgc
     1741 cggcaccgac cgttcgatgc cgatcctgca ggtggcgcgg atgtcgttca tcccggtggg
     1801 cctgccctcg gaactcggtg tcgggctgca gggtgccgga gccttctcgc cgggcctgcc
     1861 gagctttgcc aatggctgcc atatctgcga ggtcgaggtc gaccccgaaa ccggcgaggt
     1921 ggcgctcgac cgctacaccg tcgtcgacga cattggcacg gtgatcaacc ctttattggc
     1981 gacggggcag atccagggag gtgcggcgca aggtgtcggc caggcattga tcgaagacat
     2041 tgtctacggc cgcgacaatg gccagttgct gaccggctct ctcttggatt acggcattcc
     2101 gcgcgccgac atgctgccgg caatcgccgt cgagttcagc cccgtcccga gcacgaccaa
     2161 cccgctcggc gtcaagggcg tcggcgaagg cggtacgatt gccgcgacac cgacggtgat
     2221 caacgcaatc ctcgacgccc tcgccccgct cggggtcctc gatataccga tgcccgccac
     2281 ccccgaacgc atctggcgcg ccatccgcga gcgcgcctag cgttggggtt ttccaacctg
     2341 gtcagcctcc tcttcatcgt catcactacg gccgcgacgc taaatgcgca cggcatcacc
     2401 gacatccaga cctcttcgca agcgttgccg tcgccgcgat gttttttgac gcggtgatct
     2461 ctcggctaat gacgaaaacc gaacagcgct tgcgcgttgt ggtgcaggat cttgtcggta
//
LOCUS       DATLJT010001701         1704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3400171,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001701 DATLJT010000000
VERSION     DATLJT010001701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3400171"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /gene="gatC"
                     /locus_tag="VM910_38740"
     CDS             <1..211
                     /gene="gatC"
                     /locus_tag="VM910_38740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVI68471.1"
                     /translation="LQGELNAMLAFVEQLSEVNIEGVEPMTSVTPMQMKKRADVVDDG
                     EIPDDIVRNAPATEGHFFLVPKVVE"
     gene            235..459
                     /locus_tag="VM910_38745"
     CDS             235..459
                     /locus_tag="VM910_38745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68472.1"
                     /translation="MCLLCDDEKAYQAYMNYFDAMERQGKAADPEAAVNAVLDQLEAQ
                     DKARAKLRERADDPANDKTLSPFFCSPINK"
     gene            456..>1704
                     /locus_tag="VM910_38750"
     CDS             456..>1704
                     /locus_tag="VM910_38750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI68473.1"
                     /translation="MTDFTSLTLAEARDGLAKKSFTSLELTEAHLKAMEAARVLNAFV
                     LETPDQARNMAREADNRISKGEGGPLAGIPLGIKDLFATKNVRTTACSKILGNFVPTY
                     ESTVTANLWRDGAVMLGKLNNDEFAMGSSNETSCFGPVANPWRRNGANTTLVPGGSSG
                     GSASAVAALLCMGATATDTGGSIRQPAAFTATVGIKPTYGRCSRWGIVAFASSLDQAG
                     PIGRTVKDAAILLRSMAGHDPKDTTSVDRPVPDYEAAIGKSVKGMKIGIPKEYRLDGM
                     PAEIEKLWSDGAAWLKAAGAELVDVSLPHTKYALPAYYIVAPAEASANLARYDGVRYG
                     LRVPGRSIGEMYESTRADGFGAEVRRRVMIGTYVLSAGYYDAYYLRAQKVRTLIKKDF
                     EDCFAKGVNAILTPATPSAAFGLG"
BASE COUNT          339 a          509 c          544 g          312 t
ORIGIN      
        1 cctgcagggc gagctcaacg cgatgctggc cttcgtggag caattgtcgg aggtcaatat
       61 cgagggggtc gaaccgatga cctcggtgac gccgatgcaa atgaagaagc gggccgacgt
      121 ggtcgacgac ggcgaaatcc ctgacgacat cgtcaggaat gcgcctgcga ctgaaggcca
      181 tttctttctg gtgccgaagg tcgtcgagta atcctttatc ttgcgggaag tccgatgtgc
      241 ctgctctgcg acgatgaaaa ggcctatcag gcctacatga actatttcga tgcgatggag
      301 cggcagggca aggccgccga tccggaagcc gcggtcaatg cggtcctcga tcagctcgag
      361 gcccaggaca aagcccgagc caaattgcgc gagcgggccg acgaccccgc caacgacaag
      421 acgctgtctc cgttcttctg cagtccgatc aataaatgac cgatttcaca tcgctgacac
      481 ttgccgaagc tcgcgatgga ctcgcgaaaa aatcgtttac gtcgctggag ttgacagagg
      541 cgcatctgaa ggcgatggaa gccgcgcgcg tgctcaacgc cttcgtgctg gaaacgccgg
      601 atcaggcccg caacatggcg cgcgaggcgg acaatcggat ctcgaagggt gagggcggtc
      661 cgctcgccgg cattcctctt ggaatcaagg atctgttcgc taccaagaac gtgcgcacca
      721 cggcatgttc gaaaatcctt ggcaatttcg taccgactta tgagtcgacg gtcaccgcca
      781 atctctggcg cgacggcgcg gtgatgctcg ggaaactcaa caatgacgag ttcgcgatgg
      841 gctcgtcgaa cgagacttcg tgtttcggcc ctgttgccaa tccctggcgg cgcaatggcg
      901 ccaacaccac gctggtgccg ggcggctcat cgggcggatc ggcttcggcg gtggcagcct
      961 tgctgtgcat gggcgcgacc gcgaccgata ccggcgggtc gatccggcaa cccgccgctt
     1021 tcaccgcaac cgtcggtatc aagccgacct atgggcgctg ctcgcgctgg ggcatcgttg
     1081 cctttgcgtc ctcgctcgat caggcaggcc cgatcggccg cacggtcaag gatgcagcga
     1141 tcctgttgcg ctcgatggcg ggccacgatc cgaaggacac gacctcggtc gacagaccgg
     1201 tgccggatta tgaggccgcg atcggaaaat ccgtgaaggg gatgaagatt ggcatcccca
     1261 aagagtatcg cctcgacggc atgcccgctg aaatcgagaa gctctggagc gacggcgcgg
     1321 cctggctgaa agcagcgggc gcagaactgg tcgatgtgtc gctgccgcat accaaatatg
     1381 cgctgccagc ctattatatc gtcgcgccgg cggaggcttc ggccaatctc gcgcgctatg
     1441 acggcgtgcg ctatggcctg cgtgttcccg gccggagcat cggcgagatg tatgaaagca
     1501 cccgcgccga cggctttggc gcagaggtgc ggcgtcgcgt gatgatcggc acctacgtgc
     1561 tttcggccgg ctattacgac gcctattacc tgcgcgcaca aaaggtccgc acgctgatca
     1621 agaaggactt tgaggattgc tttgccaaag gcgtgaacgc gatcctgacg ccggccacgc
     1681 catcggccgc gttcggcctc ggcg
//
LOCUS       DATLJT010001702         4733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2022493,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001702 DATLJT010000000
VERSION     DATLJT010001702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022493"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(754..2394)
                     /locus_tag="VM910_38755"
     CDS             complement(754..2394)
                     /locus_tag="VM910_38755"
                     /inference="COORDINATES: protein motif:HMM:NF023726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HVI68474.1"
                     /translation="MSDGERVVPLHPVPEIGPEERAHRLQVEVERLAQLPMAEWMLYV
                     IGTEGYAERYGVDKVTLKQMVERVIKENKQRQRAEQVEKRRVEDRAKKQREVVKREKE
                     RKDERREQQEKKDAEKKDRERQAGLDVIAKLPRGEHETGLRQLARRLGEDVEALRAEL
                     EVLLTDKEEATRRAAGEPWPEPVNTKELLDESLALLRDYFVIHDDAAATIYALSVPFA
                     WIHDEIAAHSPLLVIQAADSDVAKTNLTKALLQLMPRSRMIVQPTGPALYRLIDHHHP
                     TLGIDNGDKLLAHDHDLADIVNSSWTRGILIPRTVDKAIHEFDPFCFKIINGVDLLPH
                     LDPATRTRCITTEMRPKLTGETVINFKHAAKDERFATLRRKAMRWTTDNKAAIENAEP
                     TMPEGFHNRLAENYVLLFAIADLAGSPWSEKVRTAAVKLSREFNMPSMGRRLLAIFHD
                     LASRHGPLLTSSGIEQVLPAYGNEWANYKGSGRPITKWQVAELLAPFKIYPDVIHPRG
                     RSADRGYDTRWPKFEIAFRHYLGKETPAGCTLVRKEKK"
     gene            complement(2855..3031)
                     /locus_tag="VM910_38760"
     CDS             complement(2855..3031)
                     /locus_tag="VM910_38760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68475.1"
                     /translation="MKSSDKRATAELLIATVEAAIARGWRHYSEKGELLASTLAVLEC
                     LQREGFVTVEESKT"
     gene            complement(3441..3947)
                     /locus_tag="VM910_38765"
     CDS             complement(3441..3947)
                     /locus_tag="VM910_38765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68476.1"
                     /translation="MSDDLDLNLRIGYQVQSTAEWRREKALQFPDDTRNLRAAEELER
                     LAVEIEALEGSDIHRQISDVHDSIFRLCDEDDNVTSIDSVVWDLNEAVSAELRSIGFH
                     GGYETGTQFLEWYRDLLREKFETLLGEAVPAPDLDEQVANDPAVKAAKDAYDAAYAKA
                     FAEARKKL"
     gene            complement(3937..4374)
                     /locus_tag="VM910_38770"
     CDS             complement(3937..4374)
                     /locus_tag="VM910_38770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68477.1"
                     /translation="MPAVTSEQWAEHRRRCDEEERRQRVMVITQFGAEDPEATMHAMA
                     DEILKYRRALRLLVEAVGWADAGAPFSLISSGPYWHPLGAPLIVRLKCPTLRQRRRAP
                     TDDKGNLTPQEFFRRKRLDRRFVGAQTLNHRKAKLERSGGDVR"
     gene            4425..4517
                     /locus_tag="VM910_38775"
     CDS             4425..4517
                     /locus_tag="VM910_38775"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68478.1"
                     /translation="MPDKGLDRVFVFRLDAASGKLMPNGHRTAG"
     gene            4595..4733
                     /locus_tag="VM910_38780"
                     /pseudo
     CDS             4595..4733
                     /locus_tag="VM910_38780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          887 a         1494 c         1380 g          972 t
ORIGIN      
        1 cccgtcgtag ctggtctggc ccgcggcggc ggccttcaag atgacctcgg ccatgtgcgc
       61 tttgaccccc ggggtcacct gctccaccgg aattttggtc atcacctcgt cgaggaccgc
      121 ccgcatgatc tggatcagct ctggaccgta gctcgccatg atccgctctc ccgtttggtc
      181 gcaacaggaa tttgcatcac tctactgcgc ccactcctgc gaaagcgacg tcccaaatcg
      241 cgatcacaga cttgattccc gccttcgcgg caatgagcga tgcagccgtg cttgatggga
      301 gcagggtcgc tggttcggcg cgagcgggcc agacacaaag tcgcgagcac catcacgctg
      361 atgtgagcgt cgttgcgatt cgcaagtgat aacgacgcgg aaaaagcttg taaaatcgag
      421 ggtttatgcg cgccgtgtgc aatagatgtg aaaaatagcc gcggaggtcg tacgctcgta
      481 cactccattg gcatccgcgg atgtcgcgtg tgaaccaagc gcttcgcctc cgctgtgatc
      541 tcgccgttgc gcttgagcgc caccgcccgc ggcaccagag cggcccactg tcgaccgcgt
      601 tcgtaattcc actgctcgtc gacaccccac gtcgcataat cactgcgatg gccacgcccg
      661 gcccgaccat cgatcacccc caacgcgaac tcgcgcgtgg ccatgatcgc ctcgaccgtc
      721 gtcggagagg tcgggacctg ctggggcatg ggtctacttc ttctccttgc gtactagcgt
      781 acaacctgcg ggggtctctt tcccgagata gtggcggaac gcgatttcaa atttgggcca
      841 gcgcgtgtcg tagcctcgat cggcagaacg gccgcgcggg tggatgacgt ccggataaat
      901 cttgaagggc gcaagcagct ccgcgacctg ccacttagtg atcggacggc cggagccttt
      961 gtaattggcc cactcgttgc cgtacgccgg cagcacctgc tctatcccgc tcgatgttaa
     1021 caatggccca tgacgactgg cgaggtcgtg aaagatcgcg agcaagcgtc gacccatgct
     1081 cggcatattg aactcacgcg agagcttgac cgccgcagtg cgcaccttct ccgaccaggg
     1141 actgccagcg agatcggcaa tcgcaaatag cagcacgtaa ttctccgcca agcgattgtg
     1201 gaagccctcc ggcatggtcg gctcggcgtt ctcaatcgcc gccttgttgt cggtcgtcca
     1261 ccgcatcgct ttgcgccgca gggtggcgaa gcgctcgtcc ttcgcagcgt gcttgaagtt
     1321 gatgaccgtc tcgcccgtta gcttcggccg catctcggtc gtaatgcagc gcgtacgcgt
     1381 ggcggggtcc aagtgcggta gcagatcgac accgttgatg atcttgaagc agaacggatc
     1441 gaactcgtgg atagccttat cgaccgtgcg cgggatgaga atgccgcgcg tccaagacga
     1501 gttgacgatg tcggcgagat cgtgatcatg cgcaagcaat ttgtcgccat tgtcgatgcc
     1561 taacgtcggg tgatgatgat cgatgaggcg atagagcgcc ggcccggtcg gctggacgat
     1621 catgcgcgag cgcggcatca gctgtagcag agccttggtc aagttcgtct ttgccacgtc
     1681 ggagtcggcc gcttggatca ccagcagcgg cgaatgggcc gcaatctcgt cgtgaatcca
     1741 cgcgaaggga accgacaacg cgtagatggt ggcagccgcg tcgtcgtgga tgacgaagta
     1801 atcccgcagc aacgccaagg actcgtccaa cagctccttg gtgttgaccg gctcaggcca
     1861 cggctccccc gccgcgcgcc gagtcgcctc ctccttgtcg gtgagcagga cctcaagttc
     1921 ggcgcgcaac gcctcaacgt cttcaccgag acggcgcgcg agttgccgca acccggtttc
     1981 gtgctcaccg cgcggcaact tggcgatgac atcgagccca gcctgtcgct cgcggtcctt
     2041 cttctcggca tccttctttt cctgctgctc gcggcgctcg tccttgcgct ctttctcgcg
     2101 tttgacaacc tcacgctgct tcttggcgcg atcttcgacc cgtcgtttct caacctgctc
     2161 ggcgcgctgc ctctgcttat tctccttaat gacgcgctcg accatctgct tgagggtgac
     2221 cttatcgacg ccgtagcgct cggcgtagcc ttcggtcccg atgacgtaga gcatccattc
     2281 ggccatcggc agctgcgcca atcgctcgac ttcgacctgc aggcggtgcg cgcgctcctc
     2341 tgggccgatc tctggcactg gatgtagcgg aacaacgcgt tcgccgtcgg acatggctga
     2401 ccaccctaat cgcgcgtgag cggccccctc gagagctcca gccaaggaag gctacgagag
     2461 agccgcagtc accgcgcgat gaaaacgtcg aattttttgc aagccttggc tggcagtgag
     2521 caacacctag gctcaatgcc gcaaaataca acttgaatgc acacgtttag cgctgcgcca
     2581 tccccatctc gatagcgcat cgatggcgca tcgatggctc aacgagctgc gccatcactt
     2641 atccccggcg caatcgatgg cgcaacgatg gcgcaaattg aggaaaaaaa aatttgtgcc
     2701 acgtcggcgc gaggcgtcct atgcgcagtc gagggtcgac cggaaacgcc agcggccccg
     2761 gcgcgagtga tgatcccgcc gaggccgttg tggtaagccc gaaaatccta ggacagcatg
     2821 aatggtacgc gcgcgagcat cagtcaggca aggtttaggt cttcgactcc tcgacggtca
     2881 cgaagccctc ccgctgcaag cactcgagca cagcgagcgt cgaggccagc agctcaccct
     2941 tctcgctgta atgtcgccac ccgcgcgcga tcgccgcttc gacggtggcg atcaggagct
     3001 cggccgtcgc ccgtttgtca ctcgatttca tcgtcgcgcc gccgatccag gtgagccgtt
     3061 catcgccgga gccatccgcg ccaacaaggt ccccaacaac cgactcgatc agggcgaagc
     3121 ttattcccgg cgactccgat gatttccggc gcagctatag ccgttggcta tcgcctacgc
     3181 cgagacgacc cctccgaatc cagattaaac cgcgacgcat gcccgcagcc tacgccgcag
     3241 gcggcgcgac gtcaggcact tcaggcgtgt catcgatgct gtgtctcggc cgccagcgag
     3301 gaccgggggc gatcagcgcg aacggcgcgg ctgcggccac cattggaaat tgttcgccac
     3361 aaaacctctt ttgccgcgtc taacttcttc cgtctcggga gacgcgccct tggtcgccgt
     3421 cagggcttgt gttaccggct tcacaatttc ttacgtgctt ccgcgaacgc cttggcatac
     3481 gctgcgtcat aggcgtcttt ggccgctttt actgcggggt cgttagccac ttgctcgtca
     3541 agatcaggag cgggaactgc ttcaccaagc agcgtttcga atttttctcg cagcagatcg
     3601 cgataccatt ctagaaattg ggtacccgtc tcgtagccac catgaaagcc gatcgagcga
     3661 agctcggcag atacagcttc gtttaaatcc cacacaacag agtcgataga agtcacgttg
     3721 tcgtcttcgt cgcacagcct aaaaatgctg tcgtgtacgt cgctgatctg ccggtgaata
     3781 tctgaacctt caagcgcctc aatctccaca gcgaggcgct ccagctcctc ggctgctctc
     3841 agattgcgcg tgtcgtcggg aaattggaga gccttttcac gccgccattc ggccgtgctc
     3901 tgtacctggt agccaatgcg tagattaaga tcgagatcat ctgacatcgc cgccgctcct
     3961 ttcgagttta gctttgcgat ggttgagagt ttgggcccca acgaatctgc ggtcaagacg
     4021 cttcctgcgg aaaaattcct gcggagtgag attacccttg tcatctgtcg gcgcgcggcg
     4081 tcgctggcgc aacgtcgggc atttcagtcg cacgatcaat ggtgcgccga gcggatgcca
     4141 atagggcccg gaactgatca gcgagaacgg cgcgcccgca tctgcccaac cgaccgcttc
     4201 tacgagcagc cgcagggctc ggcggtactt cagaatctca tcggccatcg catgcatcgt
     4261 cgcctcggga tcttcggcgc cgaattgtgt gatgaccatc acacgttgcc ggcgctcctc
     4321 ttcgtcgcac cgacgccgat gctcggccca ctgctccgac gtaacagcag gcaagctcgc
     4381 atcctcacga cgctgtgttt gatccgagcg gcgggttcat cgctgtgccc gacaaggggc
     4441 ttgaccgggt cttcgtcttt cgcctcgacg cggcgagcgg gaaactcatg ccgaacggcc
     4501 acaggactgc cggttgagcg ctgtgatacg cccgtttttc gccgtggtcc aattgccgct
     4561 agtcccggtc caaatgtcct gctgtgacat ggatgtggcg atggaagcga ccggggtgta
     4621 ctggaagccg gtgtggcagg tactggcgga tggtgccttc gaactcattt tggcaaacgc
     4681 ggcacaggtg aagagcgtgc ctggtcgcaa gaccgacgta aatgatgcca cat
//
LOCUS       DATLJT010001703         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_184233,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001703 DATLJT010000000
VERSION     DATLJT010001703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184233"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VM910_38785"
     CDS             <1..442
                     /locus_tag="VM910_38785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HVI68479.1"
                     /translation="SDIEQATRLARMMVTRWGLSEELGTVSYGENQDEVFLGMSVSRT
                     QNASEATVQKIDSEIKRLVEQGYNEANRILTEKRADLETLAKGLLEFETLTGEEITDL
                     LKGKKPNRESVLEPTTPRASAVPPAGKPRPRPDPDAGLEPQPQA"
     gene            complement(553..1407)
                     /locus_tag="VM910_38790"
     CDS             complement(553..1407)
                     /locus_tag="VM910_38790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68480.1"
                     /translation="MSALRNLLAAIMLIASAAAAHAAPQWLTLPATPTLPSAAQSGLA
                     PVNGIKIWYASFGRGEPVLLIHGGLANSNYWGHQVRALQKHYRVVVMDSRGHGRSSRN
                     EQPYGYDLMASDVLALMDHLGIKKAAIVGWSDGAIIGLDIAMHHPERVSRLFAFAANS
                     DPSGVADIAKSDVFNAYIERAGEEYKRLSPTPTEYKSFVEQITKMWETQPNWTEADLA
                     AIKVPTWIVDADHDEAIKRENTEFMAAHIPGAGLLIQPNVSHFSFMQDPEQFTNDVLH
                     FLEHVPAR"
     gene            1530..2528
                     /locus_tag="VM910_38795"
     CDS             1530..2528
                     /locus_tag="VM910_38795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68481.1"
                     /translation="MERRAFTMMLGCAMVIAVAVPSSPLRAQQGAIPVVGFLNGGLAR
                     NYAPLATAFVKGLEEAGFVEGRDVTIEYRWAEGQNDRLPALAAELVQRNVAVLAATST
                     PAALAAKGLSTTIPIVFETAADPVGLGLVASLNRPGGHITGVTQLSVETGPKRLEVLH
                     ELFPDRRAVALLVNPSNPVIAETAAREMQPAARTFGMELHVLKATADDDLDTAFTEAA
                     RLQVSGLVISGGDPFFVSRTERMAALAASHAVPAVGACRAFVAAGGLLSYGGDIVDAY
                     RLTGNYVGRILKGEKPADLPVQQATKIELAVNLKAAKALGITVPLTLLGRADEVIE"
     gene            complement(2571..>2999)
                     /locus_tag="VM910_38800"
     CDS             complement(2571..>2999)
                     /locus_tag="VM910_38800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVI68482.1"
                     /translation="LLHAVPGVELVDLKQPAVGLQSVNLGVLPAYKRELQRAELDAAR
                     AARVDALVAIYHSDHRELCAHEREQPFRIVNILEVIGESMGLRRHDRYKELKITQDAD
                     LIMRECGDLIAAHALDSAQAREVVAKALIGDQPLPLGKRT"
BASE COUNT          525 a          945 c          997 g          532 t
ORIGIN      
        1 gtccgacatc gagcaggcca cgcggcttgc gcgcatgatg gtgacccgct ggggcctttc
       61 cgaagagctc ggcaccgtgt cctatggcga gaaccaggac gaggtgtttc tgggcatgtc
      121 ggtgtcgcgc acccagaatg cctcggaagc gaccgtccag aagatcgact cggagatcaa
      181 gcggctggtc gagcagggct acaacgaggc caatcgcatt ctgaccgaga agcgcgccga
      241 tctcgagacg ctcgccaaag gcctgctcga gttcgaaacg ctgaccggcg aggagatcac
      301 cgatctcctg aagggcaaga agcctaaccg tgagtcggtg ctcgagccga ccacgccgcg
      361 cgcgtccgcc gttccgcccg ccggcaagcc ccggccgcgt cccgatccgg acgctggcct
      421 tgagccgcag ccgcaggcgt aagtccttat ggccggctgc tccggccgct ccaatcgtgc
      481 aaaagcccgg caacaagccg ggctttcgtt ttttgagagg ggcgggttcg cgaagggcgc
      541 cgcccccgcg gctcaccgcg ccggcacgtg ctcgaggaaa tgcagcacgt cgttggtgaa
      601 ttgctcggga tcctgcatga acgagaagtg gctgacgttc ggttggatca acaggcccgc
      661 acccggaata tgcgcggcca tgaactcggt gttctcgcgc ttgatcgcct cgtcatgatc
      721 ggcatcgacg atccaggtgg ggaccttgat ggcggcaaga tccgcctcgg tccagttcgg
      781 ctgggtttcc cacattttgg tgatctgctc gacgaagctc ttgtactcgg tgggcgtcgg
      841 cgaaaggcgc ttgtactctt cgccggctct ttcgatgtag gcgttgaaca catcgctttt
      901 cgcaatgtcg gccacgcccg acgggtcgga gttcgcggca aacgcaaaca gtcttgaaac
      961 gcgctcggga tgatgcatgg cgatgtcgag gccgatgatg gcgccgtcgc tccaacccac
     1021 gatcgccgcc ttcttgattc cgagatgatc catcaaggcg agcacgtcgg aggccatcag
     1081 gtcgtaaccg tagggttgtt cgttgcggct cgagcggccg tggccgcggc tgtccatgac
     1141 gacgacccgg taatgcttct gcagcgcgcg aacctgatgg ccccaataat tggaattggc
     1201 gagcccgcca tgaatcagca acacgggctc gccccggccg aagctggcgt accagatctt
     1261 gatgccgttg accggcgcaa ggccgctctg ggccgcactg gggagcgtcg gcgtggccgg
     1321 aagagtcagc cattgcggtg ccgcatgtgc cgccgcagcc gacgcgatca gcatgatcgc
     1381 ggcgagaaga tttcgtaaag ccgacatggc gccctcctgc tcgcaaatct cactccgtac
     1441 cagcacggac cggcgcaggt gaagcgattg cgccgttcgt cggtcccgtc aaggcatgat
     1501 aggctgttgc cggaggcgaa ggggtgacca tggagcggcg cgcgttcacc atgatgctcg
     1561 gctgcgcaat ggtgattgcg gtcgctgtgc cgtcgtcacc gttgcgcgcg cagcagggcg
     1621 cgatccccgt ggtcggcttc ctcaatggcg gactggcgcg gaactacgcg ccgctcgcga
     1681 cggcctttgt caaggggctc gaagaggccg gtttcgtcga aggacgagac gtcaccattg
     1741 aataccgctg ggccgagggc cagaacgatc gtctgcctgc attggcggcc gaactggttc
     1801 aacgcaacgt cgcggtgctt gcagccacgt cgacaccggc ggcgctggct gcaaagggat
     1861 tgagcacgac gatcccgatc gtgttcgaaa ccgcggccga tccggtcggg ctcggccttg
     1921 tcgccagcct gaaccggcca ggcgggcata tcaccggcgt gacccaattg tcggtcgaga
     1981 cgggaccaaa gcggcttgaa gtgctgcacg aactgtttcc cgatcgccgc gcggtcgcgc
     2041 tcctggtcaa cccgagcaat cccgtgattg cggagaccgc tgcaagggaa atgcagcccg
     2101 cagcccgcac ctttggcatg gaactgcacg tgctgaaggc gacggccgat gatgatctcg
     2161 atactgcctt caccgaagca gctcgactgc aggtgagcgg gctcgtgatc agcggcggcg
     2221 atccgttctt tgtcagccgg accgaacgga tggccgcgct tgcggcgagc cacgccgtgc
     2281 cggcggttgg tgcctgccgc gcatttgtcg ccgccggcgg cctgctcagc tatggcggcg
     2341 atatcgtcga tgcctatcgt ctcaccggaa attacgtcgg ccgcattctc aagggagaga
     2401 agccggccga cctgccggtt cagcaggcga ccaagatcga gcttgcagtc aatctcaagg
     2461 ctgcgaaggc gcttggcatc accgtgccgc tgaccctgct cggccgcgcc gacgaggtga
     2521 tcgaatgaag cggcggcacg tttcggccct tgccgcagtt gcaacaggtc ctacgtcctc
     2581 ttgccgagcg gcaagggctg gtcgccgatc agcgccttcg cgacgacctc gcgcgcctgc
     2641 gccgaatcga gcgcgtgcgc ggcgatcaaa tcgccgcact cgcgcatgat cagatcggca
     2701 tcctgggtga ttttgagctc cttgtagcgg tcgtggcgac ggaggcccat gctctcgccg
     2761 atgacctcca ggatgttgac gatgcggaac ggctgctcgc gctcgtgggc gcagagctcg
     2821 cgatgatcgc tgtgatagat ggcgaccagc gcatcgacac gcgccgcgcg cgcggcgtcg
     2881 agctcggcgc gctgcagttc gcgcttgtag gcgggcagca cgccgagatt gacgctctgc
     2941 agcccgacgg ccggctgctt gaggtcgaca agctcgacgc cgggcacggc gtgcaaaag
//
LOCUS       DATLJT010001704         9638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3173503,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001704 DATLJT010000000
VERSION     DATLJT010001704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173503"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VM910_38805"
     CDS             complement(<1..588)
                     /locus_tag="VM910_38805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVI68483.1"
                     /translation="MHAMVLQASGKPLTLTERPDPTPGPGEVRVKVSACGVCRTDLHV
                     VDAELPDIRYPIVPGHEVVGRVDALGDGVSDLALGMRVGVPWLGFTCGACPYCRRHEE
                     NLCDRPQFTGYTRDGGFASHLVADARYCFPLGEDGDDAELAPLLCAGLIGWRSLVMAG
                     SGKTLGIYGFGAAGHIVAQVARWQGRTVYAFTRKGD"
     gene            810..1205
                     /locus_tag="VM910_38810"
     CDS             810..1205
                     /locus_tag="VM910_38810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVI68484.1"
                     /translation="MTQTVKSVTPETTVGDLYRLFAADDFDAYPVVRNDTVVGVVSKL
                     DALKVFAFAKDQILPHYADGMGATVNEIMTAEVIAVDPETRLQRVLQLLVEHRVKSVP
                     VIDKWRNLVGIIAREDVMRAMARCMRQAE"
     gene            1233..3977
                     /locus_tag="VM910_38815"
     CDS             1233..3977
                     /locus_tag="VM910_38815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HVI68485.1"
                     /translation="MSTYGLERVFAPRTLALVGGSSRPSSLGAKILQNLLRGGFAGDI
                     AVVNPKYGLIDGKNTYPDLASVPFVPDLIVITAPASAVPGIVAEAGRLGVAGAVIISS
                     GLGHGEGSPGEAVLKTARDQKVRIIGPNCLGIMFPRCGLNASFAAHQPGKGKLALISQ
                     SGALAAAMVEWGAERAIGFSGIVSIGDQLDVDIADLLDYFALDDGTHAILLYVEAVRD
                     ARKFMSAARAAARLKPVVVVKSGRMAQGAKAAETHTGALAGSDAVYDAAFRRAGLLRV
                     FDLRELFDCVELLGQSRAPAGKNLTILTNGGGLGILAVDRLAELGGNATALAAATKAK
                     LDAVLPATWSKANPVDIGGDADSVRYLAALDVLLADTAGDAVLIMNVETAVEPSGDIA
                     RAVAERIKADRARRGASAKPVLAAWVGTDQNAAPIFDDAAIPHFPTEDDAVRAFMHLV
                     KHREAIAALMATPPTVSSLFKPDRDGAKKLIVNALADGRNWLDPVEISALFAAYEIPM
                     VPTLGASSPDEAATMAAPFLDEGRAVAVKIRSRDIRHKSDIGGVMLNLATRESVRDAA
                     AEVMARARRARPDATIEGVTIQPMISRSGSRELLLGLADDPTFGPVVVFGHGGTAVEV
                     IKDKALALPPLDMNLARDLVGRTAVARLLAAYRDVPAVPADDVPLTLVKLSQMAADLP
                     EISGLDINPLIADGDGVMALDGRVAIKVPEKFFVGRTRLAIRPYPAEWERQLQLAGDW
                     KVAVRPMRPEDEPAIAALLTRVSREDLRLRFFHAMKEFSHAFIARLTQLDYARAMAFV
                     ASDVATGDLIGVVRLHSDSHYETAEYAILLRSDLKGRGLGWALMQLLIEYARAEGLKR
                     LTGAVLRDNATMLAMCRELGFEVKSDPRDSAVAIVSLDLAVPSPVPPAADRKAVAAQP
                     "
     gene            complement(4049..4528)
                     /locus_tag="VM910_38820"
     CDS             complement(4049..4528)
                     /locus_tag="VM910_38820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVI68486.1"
                     /translation="MTTSHLIGHVRVLWRTERIIAELRLKRLLTNLGLQALAALIMAC
                     ALLLFELAAYFSLVQVWNAIWSVVALGAFNLLLAGVIVLFALRRPQTRELALADEIHQ
                     QALAAFEADLQKAESDSPASLRAAIEGAAIPLLVPLIPLLIKRLRRQATEKAEQEAA"
     gene            complement(4525..4803)
                     /locus_tag="VM910_38825"
     CDS             complement(4525..4803)
                     /locus_tag="VM910_38825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68487.1"
                     /translation="MAYRNDYVDELRSLKQETTRLTGAQAEEWREVSSQKAKDVADDV
                     KAFLSDLRDAIALDEKEIERAFTGRAAAALMTAVAVGVVIGWVLRKKP"
     gene            4962..6074
                     /locus_tag="VM910_38830"
     CDS             4962..6074
                     /locus_tag="VM910_38830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HVI68488.1"
                     /translation="MTNPPQLTESGDKSTTLHSSGAWTAANVEALERLCESAPAGRVG
                     AIDLSGVSALDTTGAWLFEKLARRFSTPSAQIAFVGVPESYTGLMREMQGVNRREPLA
                     RRHGSALLDFIERVGRGLAAETASFLQMLGAVGIALLGVLRHPRSLRLTSTIYQLYRV
                     GWQAIPIMVLITFLIGAIIAQQGFFHFRKFGAESYTVDMVGILVLREIGVLIVAIMVA
                     GRSGSAYTAELGSMKMREEIDALSTMGLDPVAVLILPRVAALVIALPILTFIGSIAGL
                     YGGGVVAQFYGDMAPAIYIERLREAVSLTTFKVGILKAPFMALVIGIVACSEGLRVQG
                     SAESLGKQTTTSVVKSIFLVIVLDGLFAVFFASIGM"
     gene            6077..6823
                     /locus_tag="VM910_38835"
     CDS             6077..6823
                     /locus_tag="VM910_38835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI68489.1"
                     /translation="MPAETIAIHVRDLVVGFGGHTVLDHLSLDVRAGEIIGLVGASGG
                     GKSVLMRTLIGLLPKRGGQIEFPATADGEVGRRGVLFQQGALFSALNARQNIQFPLRE
                     AAHVSEGLLDEIANAKLEMVGLSADDGDKFPSELSGGMTKRVALARALALDPALLFLD
                     EPTSGLDPIAAGEFDSLIKTLQRTLHFTVFMITHDLNTLAAVCDRVAALAEGRIVALG
                     PLSSVLKSDHPWVKSYFHGARAQMLHLETT"
     gene            6841..7947
                     /locus_tag="VM910_38840"
     CDS             6841..7947
                     /locus_tag="VM910_38840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVI68490.1"
                     /translation="METRAPYIIVGAFVLSAIVAVFGFVYWLNNTGGIGKRDTYQLVF
                     NGPVPGLLVGAAVLFNGIRVGEVTSLELVADHPHDVHATIAVAQRTPVRSDTRVGLDF
                     QGLTGVPVVALEGGDNAQAPPVREPLVAEKGAGQSMTQAARDALRRVDTVLSDNAAPL
                     HDAIGNISTFAEALARNSSRLDGIVAGLERMTGGATPPRKVIYDLSAASGFAMPHPPL
                     PAGLAITEPTATVRLQTQRFLFTNGEEGQDDFANALWSDSLPTLVQAKLLQSFENYDI
                     AHAPLRADNLSEGGARLLIDLRQFEIDGSPEPRASISLSAKLVDGTGHLQAARIFEKS
                     APMGGLTPARAAAAFNEAFDALARELVVWTASQR"
     gene            7926..9083
                     /locus_tag="VM910_38845"
     CDS             7926..9083
                     /locus_tag="VM910_38845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HVI68491.1"
                     /translation="MDGFATIGQRDSRDGLGSGQQSDRDLRLDACRGIALWFIFLDHV
                     PNNVGSWLTLRHYGFSDTTELFMFVSGITCALAYRKVQACDGWRAVICHTIRRGFEIY
                     VAFLALTVACVVMVYLLGGGGFADETNTRVLFEHPGPALARAVILQYRPVNTDVLPTF
                     VIFHLLFAPLLWLLLNVPRLALAASFLLYALVQLFGWNLPQWPRNEWYFNPFAWQFLV
                     VLGAGWEIADRKKLRPWLMSRAMTGCAVLYLLFAFAVALSWSFKPLEAAIPQALAKFM
                     YPIDKSNLDPLRLLHFLAIAVLVAKFVPRDWKGLTTPILRSAVRCGENSLEIYCAGVL
                     LALAAHALLVRVSADIAMQVAVSAGGVLALIAIATVLTRLKVESGSEPRLF"
     gene            9256..9456
                     /locus_tag="VM910_38850"
     CDS             9256..9456
                     /locus_tag="VM910_38850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68492.1"
                     /translation="MDEPVVWETIATAPYDRDLELAVIEGDHVHPLVFACRRGPSGWI
                     KVATRERVPVSPTHWRPWQKAP"
     gene            complement(9453..>9638)
                     /locus_tag="VM910_38855"
     CDS             complement(9453..>9638)
                     /locus_tag="VM910_38855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68493.1"
                     /translation="QEPRRAMDKTVADLNIKHFKKLLASETDPVKRQTIERLLAEEEA
                     KLARALAAKGAPPDHKT"
BASE COUNT         1637 a         3027 c         3123 g         1851 t
ORIGIN      
        1 atcgcccttg cgcgtgaacg catagaccgt tcgtccctgc cagcgcgcaa cctgtgcgac
       61 gatgtgcccg gccgcgccaa agccatagat cccgagcgtc ttgccggagc cggccatgac
      121 cagcgagcgc cagccgatca gaccggcgca gagcagcggc gcaagctcgg catcgtcgcc
      181 gtcttcgcca agcggaaagc agtagcgcgc atcggcgacg agatggctcg cgaacccgcc
      241 gtcgcgcgtg taaccggtga attgcggacg gtcgcacaga ttttcctcat gccgtcggca
      301 ataggggcag gcgccgcagg taaagccgag ccatggaacg ccgacgcgca taccgagggc
      361 aagatcgctc acgccatcgc ccaacgcgtc gacccggccg accacttcat gaccggggac
      421 gatcggatag cggatgtccg gcaattcggc gtcgacgacg tgcaggtcgg tgcggcacac
      481 gccgcaagcg ctcaccttca cgcgcacctc gcctggtccc ggggtcgggt ccggtcgctc
      541 cgtcaatgtc agaggctttc cggaagcctg aagaaccatg gcatgcatga actgctcctg
      601 gcgttcgacc caattaggga gaggccaaag aagatcgttt gatcctggtc aacgttaagc
      661 ggccggcgcc ccattagtag tgtcgccgct aatttgctag actaggaggc atggcgtgcc
      721 gcctgatcgg gcggcgcgcg cgagccgacg gaactctcac gaaagaatcg gccatgtacg
      781 aatttcttca agaaactgtc gcaaataaca tgacgcaaac ggttaaaagt gtgacgccag
      841 agaccacggt gggcgacctt taccggctgt tcgcggcgga cgattttgac gcctatccgg
      901 tcgttcggaa cgatacggtg gtcggcgtgg tgtcgaagct ggatgcactc aaggtgttcg
      961 ctttcgcaaa agatcagatc ctgccgcact atgcggatgg catgggtgcg actgtcaatg
     1021 aaatcatgac ggccgaggtc atcgccgtgg accccgaaac caggctgcag cgcgtcctgc
     1081 agctcctggt cgagcatcgg gtcaagagcg tgccggtcat tgacaagtgg cgaaatctcg
     1141 ttgggattat cgctcgcgag gatgtcatgc gggcgatggc gcgctgtatg cggcaagcgg
     1201 aatagcccct gatccccctg cgagacgaaa cgatgtccac atacgggcta gaacgggtct
     1261 ttgcgccgcg cactctcgcg ctcgtcggcg gcagctcgcg accctcgtcg ctcggtgcca
     1321 agattttgca aaacctgctg agaggcggct ttgcgggcga tatcgctgtc gtcaatccga
     1381 aatacggttt gattgacggc aagaacacct atcccgatct cgcttcggtg cccttcgttc
     1441 ccgacctgat cgtgatcacg gcgcctgctt cggcggtgcc gggcatcgtg gcggaagcgg
     1501 gccggcttgg tgttgccggt gctgtcatca tctcgtcggg cctcggccac ggcgagggct
     1561 cgccgggtga ggcggtgctc aagaccgccc gcgaccaaaa ggtcaggatc atcgggccga
     1621 actgcctcgg catcatgttt ccgcgctgcg ggctcaatgc gagctttgcc gcacatcagc
     1681 ccggcaaggg gaagctcgcg ctgatctcgc aatccggcgc gctcgcggcc gcgatggtgg
     1741 aatggggtgc ggaaagggcg atcggatttt ccgggatcgt gtcgatcggc gatcagctcg
     1801 acgtcgatat cgccgacctc ctcgactatt ttgcgctcga cgacgggacc cacgcgattc
     1861 tgctgtacgt cgaggcagtc agggacgccc gcaagttcat gtctgcggcg cgtgcagcgg
     1921 cccggctcaa gccggtggtc gtcgtcaagt ccggacgcat ggcgcagggt gcaaaagcgg
     1981 cggaaaccca caccggggcg ctcgccggaa gcgatgccgt gtacgacgcg gcgtttcgcc
     2041 gggccggcct gcttcgcgtg ttcgatctgc gcgaattgtt cgactgcgtg gagctgctcg
     2101 gccagagtcg ggcccccgcc gggaaaaatc tcacgatcct taccaatggc ggcgggctcg
     2161 gcattctggc cgtcgaccgg cttgcggaat tgggcggcaa cgcgacagcg cttgcggcgg
     2221 caacgaaggc gaagctcgat gccgtcttgc cggcgacctg gtcgaaagcc aatccggtcg
     2281 acatcggcgg cgatgcagat tccgtgcgct atttggccgc tctcgacgtg ttgctcgctg
     2341 acactgccgg cgatgccgtg ctgatcatga atgtcgagac ggcggtcgag ccgtcgggcg
     2401 acatcgccag ggcggtggcg gaacggatca aggctgaccg cgcgcgacgg ggcgcgtcgg
     2461 caaaaccggt tctcgccgcc tgggtcggca ccgatcagaa tgcggcgccg atcttcgatg
     2521 atgccgcaat ccctcatttc ccgaccgagg atgatgccgt gcgggcgttc atgcatctgg
     2581 tcaagcatcg cgaggcgatt gcggcgctga tggcgacgcc acccaccgtg tcgtcattgt
     2641 tcaagcccga ccgcgatggc gccaaaaaat tgattgtaaa cgccctggcc gacggaagaa
     2701 actggctcga tccggtcgaa atatcagcgc tgtttgcggc ttacgagatc ccgatggtac
     2761 cgacgctcgg cgcaagcagt cccgacgagg cggcgaccat ggccgcacca ttcctcgacg
     2821 aggggcgcgc cgttgcggtg aaaatccgat ccagggatat caggcacaag tccgatatcg
     2881 gcggggtcat gctgaacctc gcgaccagag agagcgtccg cgatgccgca gccgaggtca
     2941 tggcccgcgc gcgtcgcgcg cggcccgacg caacaatcga gggcgttacg atccagccga
     3001 tgatttcgcg gtcgggttcg cgcgaattgc tgctcgggct tgccgacgac ccgaccttcg
     3061 gcccggtcgt ggtgttcggc cacggcggca ccgcggtaga agtgatcaag gacaaggctc
     3121 tggcgttgcc gccgctcgac atgaacctcg cgcgcgatct cgtcggccgc acggccgtcg
     3181 cgcggcttct ggcggcctat cgcgacgtgc ctgccgttcc ggcggacgac gtgccgctga
     3241 cgcttgtcaa actctcccag atggcagccg accttccgga aatctccggg ctcgatatca
     3301 atccgttgat cgcggatggc gacggcgtca tggctctgga cggccgcgtt gcgatcaagg
     3361 tgcccgaaaa attctttgtc ggccggacgc ggctcgcgat caggccctat cccgccgaat
     3421 gggaacgtca gctccagctc gcgggcgatt ggaaggtggc ggtccgtccg atgcgccccg
     3481 aagacgagcc ggccattgcc gcgcttctga cccgcgtcag ccgcgaagac ctcaggctcc
     3541 gcttcttcca tgccatgaag gagttctcgc acgccttcat cgcccgtctc acgcagctcg
     3601 actacgcgcg cgccatggcc ttcgtcgcaa gcgacgtggc gaccggcgac ctcattggcg
     3661 tcgtgcgcct gcattcggat tctcattatg agaccgccga atatgcaatc ctgcttcggt
     3721 cggacctgaa aggcaggggc ctcggctggg cgctcatgca gctcctgatc gaatatgcca
     3781 gggccgaagg gctcaagcgc ctgaccggcg cggtgttgcg cgacaatgca acgatgctcg
     3841 cgatgtgccg cgagcttggg ttcgaggtca agagcgaccc gagggattcg gccgtggcga
     3901 tcgtttcgct tgatctcgcc gtaccgagcc ctgtcccacc tgcggccgac cgcaaggctg
     3961 tcgccgcgca gccgtgacaa gtggtggagg ttcgtttact cggcctcgcg aaccgaaact
     4021 gctggccggg ttcgtatcct tcgcgaaatc aggcggcttc ctgttcggcc ttttcggttg
     4081 cttgcctgcg caagcgcttg atgaggaggg ggatcagggg cacgagcagg gggattgcgg
     4141 cgccctcgat cgcggcgcgc agggaagccg gtgaatcgct ctccgccttc tggaggtccg
     4201 cctcgaaggc ggcaagcgcc tgctggtgga tttcgtccgc aagcgccagt tcccgggtct
     4261 gcggcctgcg caacgcgaac agcacgatca cgccggcaag gaggagattg aaggcgccga
     4321 gggcgacgac cgaccagatc gcgttccaga cctgcaccaa cgagaaatag gcggcgagct
     4381 cgaacagcag cagagcgcag gccatgatca gcgctgcgag cgcctggaga cccagattgg
     4441 tcaggagacg tttgaggcga agttcggcga tgattcgctc ggtacgccag agcacgcgca
     4501 cgtgcccgat cagatggctt gtcgtcatgg ctttttcctc aagacccagc cgatcacgac
     4561 gccgaccgcc accgcggtca tgagcgcggc ggcagctcgt ccggtgaaag cgcgctcgat
     4621 ctctttctca tcgagtgcga ttgcgtcacg aaggtccgag agaaaagcct tgacatcgtc
     4681 cgcgacgtcc ttggcctttt ggctcgagac ttcccgccat tcttctgcct gcgcgccggt
     4741 caggcgcgtg gtttcctgct tcagactgcg aagctcatcg acatagtcgt tgcgataggc
     4801 catggcgggg ttccctcgag caaaaacgct aggcgggaac ccgggcgccg ccgttgatat
     4861 gtatcaaggc cggggctgcc tcctccgctt tcttgcgctt tatcaaagcg atggatgcgg
     4921 tcgacgtcta gacttttccg atcatcatgc gggagcctgg cttgaccaac ccacctcaac
     4981 tgaccgaatc cggggacaaa tcgacgacgc tccattcgag cggggcgtgg accgctgcca
     5041 atgtcgaggc tcttgagcgg ctctgcgaga gcgcgcctgc gggacgcgtc ggcgcgatcg
     5101 atctcagcgg cgtgagcgca ctggacacca ccggcgcatg gttgttcgag aaactggccc
     5161 gtcgcttctc gacgccgtct gcccagatcg cctttgtcgg ggttcccgaa tcctacaccg
     5221 gcctgatgcg tgagatgcag ggtgtcaacc gcagggaacc gctcgccagg cggcacggca
     5281 gcgccttgct cgatttcatc gagcgggtcg gccgggggct cgcggccgag actgcgagtt
     5341 ttctgcaaat gctgggcgcc gtcggcatcg cattgctggg cgtgctgcgt cacccgcgct
     5401 cgctgaggct gacatcgacg atctatcagc tctaccgggt cggctggcag gcgatcccga
     5461 tcatggtgct gatcacattc ctgatcgggg caatcattgc gcagcagggc tttttccact
     5521 tccgcaagtt cggtgccgaa tcctacaccg tcgacatggt gggcatcctc gtgctgcggg
     5581 agatcggcgt actcattgtc gcgatcatgg tcgccggccg ctccggcagc gcctatacgg
     5641 ccgaactcgg ttcgatgaag atgcgcgagg agatcgacgc cttgagcacc atggggctcg
     5701 atcccgttgc agtcctgatc ctgccccgcg tcgcggcgct ggtgattgcg ttgccgatcc
     5761 tgaccttcat cggatcgatc gcggggctct acggcggcgg tgtcgtggcg cagttctacg
     5821 gcgacatggc gcccgccatc tacatcgagc ggctgcgcga agcggtctcc ctgaccacgt
     5881 tcaaggtcgg cattctcaag gcgccgttca tggcgctggt gatcggaatc gtggcctgtt
     5941 cggagggctt gcgggtgcag ggcagcgccg agtcgctcgg caagcagacg acgacatccg
     6001 tcgtgaaatc gatcttcctg gtgatcgtgc tcgacggcct gttcgccgtg ttcttcgcct
     6061 cgatcggaat gtgaccatgc cggctgagac aatcgccata cacgttcgcg acctcgtggt
     6121 cggtttcggc gggcataccg tgctcgatca cctgtcgctc gacgttcgcg ccggcgaaat
     6181 catcgggctg gtcggcgctt ccggcggcgg caaatcggtg ctgatgcgca cgctgatcgg
     6241 attgctgccg aagcgcggcg gtcagatcga gttccccgcg acggccgacg gcgaggtcgg
     6301 acggcgcggc gtgctgtttc agcagggcgc gctgttctcg gcgctgaatg cgcggcaaaa
     6361 catccagttt ccgttgcgcg aagcggcgca cgtctcggaa ggccttttgg acgaaatcgc
     6421 caatgccaag ctcgagatgg tcgggctttc ggccgacgac ggcgacaagt ttccctccga
     6481 gctgtccggc ggaatgacca agcgcgtggc gctggcgcgc gcgctcgcgc ttgatcccgc
     6541 gctgttgttc ctcgatgaac cgacgtcggg gctcgacccg atcgcggcgg gcgaattcga
     6601 ttcgctgatc aagacgctgc aacggacgct gcatttcacg gtcttcatga tcacgcacga
     6661 tctcaacacg ctggccgcgg tctgcgatcg ggtagcggcg cttgccgagg gcaggatcgt
     6721 ggcgctgggc ccgctgtcat ccgtgctcaa gtccgatcat ccctgggtca aatcgtattt
     6781 tcacggcgcg cgagcgcaga tgcttcacct cgaaacgact tgaggcaaca ggaacaggtc
     6841 atggaaaccc gcgcacccta tatcatcgtc ggcgccttcg tcctgtcggc gatcgtggcc
     6901 gtgttcggct ttgtctactg gctcaacaat acgggcggga tcggcaagcg cgacacctat
     6961 caactcgtct tcaacgggcc ggtgccgggc ctgctggtcg gcgcggcggt gctgttcaac
     7021 ggtattcgcg tcggcgaggt cacctcgctc gaactcgttg ccgatcatcc ccatgacgtc
     7081 cacgccacca tcgcggtcgc ccaacggacg ccggtgcgat ccgacaccag ggttggcctc
     7141 gacttccagg gattgaccgg cgtgccggtg gttgctctcg aaggcggcga caatgcgcag
     7201 gcaccgcctg tgcgcgagcc gctggtcgcc gagaagggcg ccggccagag catgacccaa
     7261 gcggcgcgcg atgccttgcg ccgggtcgat acggtgctat ccgacaatgc cgcgccgctg
     7321 catgatgcga ttggcaatat cagcacgttc gcagaagcat tggcgcgcaa ttcgtcgcgt
     7381 ctcgacggca ttgtcgcagg cctcgaacgc atgaccggcg gagccacacc gccgcgcaag
     7441 gtgatctatg atctttctgc cgcgagcggc tttgcaatgc cgcatcctcc cttgccggcc
     7501 gggcttgcga tcaccgagcc gaccgccacc gtccgcctgc agacccagcg cttcctgttt
     7561 accaatggcg aggaggggca ggatgacttc gccaatgcgc tatggagcga cagcctgccc
     7621 acgcttgtgc aggcgaaact gctgcagagc ttcgagaact acgatatcgc ccacgcaccg
     7681 ctgcgtgcgg acaatttgag cgaaggcggc gcgcgcctgc tcattgacct gcgccagttc
     7741 gagatcgacg gttcgccgga accccgcgcg tcgatctcgc tctcggcgaa gctcgttgac
     7801 ggaacaggcc acctccaggc cgcgcgcatc ttcgaaaagt cggcaccgat gggcgggctc
     7861 acgcctgcgc gggccgccgc cgccttcaac gaggcctttg atgcgctggc acgggagctc
     7921 gtggtgtgga cggcttcgca acgataggac agcgtgattc tcgggacggc ctggggagcg
     7981 ggcaacaaag cgaccgcgac ctgcgcctcg acgcatgccg gggcatcgcg ctgtggttca
     8041 tctttctgga ccacgttcca aacaatgtcg gaagctggct gacgctgcgc cattacggct
     8101 tcagcgacac caccgagctg ttcatgttcg tctcgggcat aacctgtgcg ctggcttatc
     8161 gaaaggtgca ggcatgcgac ggctggcgcg ccgtgatctg tcatacgatc cgccgcggtt
     8221 tcgagatcta tgtcgcgttt ctggcgctga cggtcgcctg cgtggtgatg gtctacctgt
     8281 taggcggcgg cgggtttgcc gacgagacca atacacgcgt tctgttcgag catccggggc
     8341 cggcgctcgc gcgcgccgtg atcctgcaat atcgcccggt caacaccgac gtgttgccga
     8401 ccttcgttat ctttcatctc ctgttcgccc cgctgctctg gctgctcttg aatgtgccga
     8461 ggctcgcgct cgccgcatcg ttcctgctgt acgcgctggt tcagctcttc gggtggaacc
     8521 tgccgcaatg gccgcgcaac gaatggtact tcaatccctt cgcctggcag ttcctggtcg
     8581 tgcttggcgc cgggtgggaa atcgcagatc gcaaaaaatt gcggccgtgg ctgatgtcgc
     8641 gcgcgatgac ggggtgcgcc gtactctatc tcttgttcgc gtttgcagtc gccctgagct
     8701 ggagcttcaa accgctcgag gcagcaattc cgcaggcgct ggcgaagttc atgtatccga
     8761 tcgacaaatc caatctcgat ccgctgcggc ttctgcattt ccttgcgatc gcagttctcg
     8821 tcgcgaagtt cgtgccgcgt gattggaaag ggttgacgac accgattctg cgcagcgctg
     8881 ttcgctgcgg ggagaattcg ctggaaatct attgtgcggg cgtgctgctg gcgcttgcag
     8941 cgcatgccct gctggtgcgg gtctcggcag atatcgcgat gcaggtcgcg gtcagcgccg
     9001 gcggcgtcct ggcgctgatt gccatcgcga ccgttctgac cagattgaag gtcgagagcg
     9061 ggagtgagcc caggttgttc tgacggatcg gccgatcttg tgccggcgcg cccgcttttt
     9121 tgatgtagct caatgaagac aggagccagt tgcctttatg aataaaggtt tacggaagcc
     9181 ctccgggcgc gtgcgcgaga tgcggtggtg gcctctggac accctgaccc ggcgcggcag
     9241 ctcaggatat cagcaatgga cgaaccggtg gtgtgggaaa cgattgcaac agcgccctac
     9301 gaccgcgacc tcgagctcgc ggtgatcgag ggagatcacg tccatcccct ggtgttcgcc
     9361 tgccgccgcg ggccaagcgg ctggatcaag gtcgcaaccc gcgagcgcgt ccccgtcagc
     9421 ccgacccatt ggcgcccctg gcaaaaggcg ccctaggttt tgtggtccgg cggcgcgccc
     9481 tttgccgcca gcgcccgagc cagcttggct tcttcctcgg cgagcaggcg ttcgattgtt
     9541 tgccgcttga ccgggtccgt ctcgctcgcc aatagcttct tgaaatgctt gatgttcagg
     9601 tccgccacag ttttatccat ggcccgtctc ggctcctg
//
LOCUS       DATLJT010001705         5663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2253685,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001705 DATLJT010000000
VERSION     DATLJT010001705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253685"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VM910_38860"
     CDS             <1..460
                     /locus_tag="VM910_38860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68494.1"
                     /translation="CAMRTKVQRSHLIPVNPQNATSNDWVILLEAGTIIGPSPERLRV
                     ARSTLERKIVKKYDFMLRAEHFLVLEGHDPRIHAVFFCPQLYKYDSEKKLVPLQGDEI
                     GRLIAHAVEGGITERRPWYDPSEKLPDRPIIDAPMNTEFDTLVVDMLSRA"
     gene            complement(722..1804)
                     /locus_tag="VM910_38865"
     CDS             complement(722..1804)
                     /locus_tag="VM910_38865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HVI68495.1"
                     /translation="MQSTDFVVARNDFKQCKLIETHLPDPDALPDDTLLVKVDRFAFT
                     ANNITYAILGERLSYWRLFPAPADFGNIPVWGFGDVIASKNPAVPEGERLFGYFPMAT
                     HLVIEAGNVSKRGLRDTAAHRQAVAPVYNAYARVANDPAYAGRQGDYQALVRPLFMLS
                     FLVDDYLAENEFFGARSVMLSSASSKTASGLAHLLHGRKKIRLIGLTSAGNADFVRSL
                     GCYDEVIAYDRVSTLPADTPTAFVDMAGNADLRARLHRHFGDALKYSGQVGLTHQNSA
                     AEEAELPGAKPAWFFAPDHIRKRTREWGPGAIDARLSAAWAGFAPTLDQWLKVIESRG
                     PTAVRQTYLDTLGGRIPPDQGNILKL"
     gene            complement(1770..2543)
                     /locus_tag="VM910_38870"
     CDS             complement(1770..2543)
                     /locus_tag="VM910_38870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HVI68496.1"
                     /translation="MTHHLNSQTDLEEAIHALVKRDPRLKPILDVAGMPSLRRREPGF
                     VGLAHIICGQQLSTASAAAIWARLSGAFDPFDHDACRRARADRLGRLGLSAAKIKTLK
                     SLARAIADERVNLDVLAEEDADSAHHTLTALHGIGPWTADVYLLFCLGHGDAWPAGDL
                     AVQEAIRIGLGLKDRPNAKQMAPIAEPWRPMRGAAAHLWWSYYRATKKREGVIAGEDK
                     TNVVSNIVAVANPRHGAKIAARKEPGSKHAVNRLRRRPK"
     gene            2581..3450
                     /gene="gluQRS"
                     /locus_tag="VM910_38875"
     CDS             2581..3450
                     /gene="gluQRS"
                     /locus_tag="VM910_38875"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HVI68497.1"
                     /translation="MPPVFRFAPSPNGYLHLGHAFSALLNFDLARASGGRLLLRIEDI
                     DPTRCRPEYEEAIYQDLAWLGISWEAPVRRQSEHLDAYRKTLDRLAAQGLIYPGFESR
                     ADIAKLVRQREAEGPWPRDPDGAPLYPGEAKLLSEDARAGLVESGVPYALRLDMSAAI
                     ARAGELSWAECGEGPNGETGRLVAAPQVWGDVVLARKETPTSYHLSVVVDDALQGVTD
                     VVRGADLFHATSVHRLLQQLLGLPEPVYRHHRLICDEAGHKLAKSTRSTALRELRAQG
                     ATAADIRTLIGLP"
     gene            3538..4785
                     /locus_tag="VM910_38880"
     CDS             3538..4785
                     /locus_tag="VM910_38880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68498.1"
                     /translation="MAARSRFVRSKRSPKKRPARKRPAAGKARKSAAAPGVVEAALAV
                     FAHEVRTPLTGILAISDLLATSDLPERERRWADTIKAGAEHLASLATLFVDAAKDRNA
                     ATAIREDLFDLRALARSAGDSLAGRAAAKGLQSRIDLSEALPSLVVGDPVRLRAALEN
                     LIDNAVKFTETGIVALDVAPARPTKRSGKGKVAVAFAVSDSGIGLTLTEIKRLFRPFS
                     QANVTIASRFGGAGLGLSSVKQIARSMGGDIVVTPRRGGGTTFTLTVALAGAEMADRS
                     RPGTGVRRPPRRFSAMRILSVEDNPFGRVVLNTILSELGHQAEFIGRGEDATERIAQG
                     AFDAVLMDMVLPGIDGIEAIRRIRALPPPLGRIAIIGVSGRSDDEAASRAAGADAFLL
                     KPVSPRALATALLEATRRSAGAT"
     gene            complement(4712..5605)
                     /locus_tag="VM910_38885"
     CDS             complement(4712..5605)
                     /locus_tag="VM910_38885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVI68499.1"
                     /translation="MRLFRYLFNIGLDAFYTFLADDGWAIASHIALSTLMAMFPFLIV
                     LTSLAGFFGSKELADEAAALLLQTWPKQVAATLSGQIHDVLTTTRTDALTIGAVLSVY
                     FASNGVEALRVALNRAYAVVETRRWYWLRLESIAYTLVAAVTALAMAFLIILGPLIME
                     AMRSYVPLVVEANRSFFHFSRYGITIAAMIVALFILHAWVPAGRRSFLQILPGIVFTL
                     VASLIAGIVFGQYLTRFANNYVTMYAGLASVIIALVFLYFIAAIFVYGGELNAAIIKS
                     RLPNGVSLQAAQSLEPAETQA"
BASE COUNT         1085 a         1751 c         1801 g         1026 t
ORIGIN      
        1 ctgcgccatg cgaacaaaag tgcaaagaag tcatctaatc ccggttaatc cacaaaacgc
       61 cacaagcaac gactgggtta tcttgctaga ggctggcacc atcattggtc cgtcacctga
      121 aagacttcgt gttgccagat caactctgga acgaaaaatt gtaaaaaagt acgactttat
      181 gcttagggcg gaacatttcc ttgtgttgga agggcatgat ccacgaattc atgcggtgtt
      241 cttttgccct caactttaca aatatgattc tgaaaaaaaa ctcgtgcctc tacagggcga
      301 cgagatcggt agattgattg ctcacgccgt tgagggcggg attaccgaga ggcgaccatg
      361 gtatgatcca tcggaaaaat tgccagatcg gccaataatc gacgctccaa tgaacacgga
      421 atttgacacg ctcgttgttg acatgcttag cagagcgtga acgtgccgtc gtcaccagcg
      481 taggctggga atgagtttca acaagtagcc gaagtattga gttgaacgcg ccgtcaatat
      541 tcaccgtcgc atcgaaatag agaaaggctc tgcgcaatgc agaacctttc ttgctcgtca
      601 gctgtcaatc ttttcgcgac cccggtcgcc cccaagaccg gggtcgttgg caaccgagtg
      661 acttattaat tgacttcact cggtggcggc gtagccgcat caccggatcg atccgagtta
      721 cctatagttt gagaatattc ccctgatccg gcggaatacg tccgccgagg gtatcgagat
      781 aggtctgcct gaccgcggtg gggccgcggc tttcgatcac cttgagccac tggtcgagcg
      841 taggcgcgaa acccgcccac gccgcggaca atcgtgcatc gatggcgccc ggtccccatt
      901 ccctcgtgcg cttgcggata tggtcgggtg cgaagaacca tgctggtttg gcgcccggca
      961 gttccgcctc ttcggctgcg gagttctgat gcgtcagccc gacctggccg gaatatttga
     1021 gcgcgtcgcc gaaatgtcgg tgcagccgcg cgcgcaaatc cgcattccct gccatgtcga
     1081 cgaaggcggt cggcgtgtcg gccggcagtg tggagacgcg gtcataggcg atgacctcgt
     1141 cgtagcagcc gagcgagcgg acgaaatcgg cgttgcccgc cgacgtcaac ccgatcaggc
     1201 ggattttttt ccggccgtgc aggagatgcg ccagtccgga cgccgtcttg ctggaagcgg
     1261 acgacagcat cacgcttcgc gcgccgaaaa attcgttctc ggcgagatag tcgtcgacca
     1321 ggaacgacag catgaacagc gggcgcacca gcgcctgata gtcgccctgg cggccggcat
     1381 aagcgggatc gttggcgacg cgtgcatagg cgttatagac cggcgcgacc gcttgccggt
     1441 gggccgcggt gtcgcgcagg ccgcgcttcg agacattgcc ggcctcgatc acgagatgcg
     1501 tcgccatcgg gaaatagccg aacaggcgct cgccttcggg cacggcggga tttttggagg
     1561 cgatcacgtc gccaaagccc cacacgggga tgttgccgaa atcggctggc gccgggaaca
     1621 accgccagta actcaaccgc tcgccgagta tcgcgtaggt gatgttgttg gcggtgaaag
     1681 caaagcgatc gactttcacc agcagcgtat catcgggcag cgcgtccgga tcgggcaggt
     1741 gcgtctcgat cagtttgcat tgcttgaaat catttcgggc gacgacgaag tcggttgact
     1801 gcatgtttcg atcccggctc ttttcgcgcc gcgatcttcg cgccgtgccg cggatttgca
     1861 acagcaacaa tgtttgaaac aacgttcgtc ttgtcttcgc cggcgatcac accttcgcgt
     1921 ttcttcgtcg cgcggtaata actccaccac aaatgcgccg ctgcgccgcg catcggccgc
     1981 cagggttccg caattggcgc catttgcttg gcgttgggcc ggtccttcag cccgaggcct
     2041 attcggatcg cctcctgaac cgcgaggtcg ccggcaggcc aggcgtcgcc atggccgagg
     2101 cagaacagca gataaacgtc ggcggtccag ggaccgatgc cgtgcagcgc cgtcaacgtg
     2161 tggtgagcgg aatcggcatc ctcctccgcg agcacgtcga ggttgacacg ctcatccgca
     2221 atcgcgcgcg ccagcgattt cagcgtcttg atttttgcgg ccgacaagcc gagccggccg
     2281 agccggtcgg cgcgggcacg acggcaggca tcatggtcga aggggtcgaa ggcaccgctg
     2341 agccgcgccc agattgccgc agcgcttgca gtggagagct gttggccgca gatgatgtga
     2401 gcaagcccca caaagccggg ctcgcgccgg cgcaggctcg gcatgcccgc gacatcgagg
     2461 atcggcttta gtcgcgggtc gcgcttgacc aaagcgtgga tcgcttcttc aaggtcggtc
     2521 tggctgttga ggtgatgggt cattgcgacc taaaaccatc acgcctgacc gaagagatca
     2581 atgccacccg ttttccgatt tgccccgagc ccgaacggct accttcacct cggccacgct
     2641 ttctcggcgt tgttgaactt cgacctggcg cgtgcgtcgg gcggccggct tttgctccgt
     2701 atcgaggaca tcgatccgac ccgttgccgg cccgaatatg aagaggcgat ctaccaagat
     2761 ctcgcgtggc tcggcatctc ctgggaagca ccggtgcggc ggcagtccga acatctcgat
     2821 gcttaccgca aaactctcga caggctcgcc gcccagggat tgatctatcc aggtttcgag
     2881 agccgtgccg atatcgcaaa gcttgtgagg cagcgggaag cggaaggacc ctggccgcgc
     2941 gaccccgacg gcgcgccgct ctatccgggc gaggcgaaac ttctgtcaga ggacgccagg
     3001 gcgggtctcg tcgaatcagg cgtgccctac gcgttgcggc tcgacatgtc tgcggcgatc
     3061 gcgcgtgccg gcgaactgag ctgggccgaa tgcggcgagg gacccaacgg cgagacgggc
     3121 cgacttgtcg ccgcgccgca agtctggggt gatgtcgttc tggcgcgcaa ggagacgccg
     3181 accagctatc atctttctgt cgtcgtcgac gacgcgctgc agggcgtgac cgacgtcgtc
     3241 cgcggcgccg atctgttcca cgcgaccagc gtgcatcggc tgctgcagca gctactcggt
     3301 ctccccgaac cggtctatcg ccaccatcgt ctgatttgcg acgaggccgg acacaagctc
     3361 gccaaatcca cccgatcgac cgccttgcgc gaactgcgtg cccagggtgc gacggccgcc
     3421 gacatccgca cgctgatcgg tcttccctga caaattccgc aagccaatca aaaggcgtcc
     3481 gtgacaccgg cagccctgcc gtgccatggt gcggcgggct cgggggccgg gagtatcatg
     3541 gcggcaagat cgcgctttgt gcgctctaaa cgctcgccga agaagcggcc ggcgaggaag
     3601 cgtcctgccg cgggcaaggc gcgcaaatcc gccgcggcgc ccggcgtggt cgaggcggcg
     3661 ctcgccgtct tcgctcacga agttcgtaca ccgctcaccg gaattctcgc catcagcgat
     3721 ttgctcgcca cctcggacct tcccgagcgc gagcgacgct gggccgacac catcaaggcc
     3781 ggagcggagc atctggcaag ccttgcgacc ctgttcgtcg atgcagccaa ggatcgcaac
     3841 gccgcgaccg cgatccgcga ggacctgttc gatctcaggg cactggcgcg cagcgccggc
     3901 gactcgttgg ccggccgggc ggcggcgaag ggattgcaat ccagaattga tctgtcggag
     3961 gcgctgccct cgcttgtggt cggcgacccc gttcgcctgc gggctgcgct tgaaaacctg
     4021 atcgataacg cggtgaaatt caccgagacc ggcatcgtcg cgctcgatgt ggctcccgcg
     4081 cggccgacga agcggagcgg caagggcaag gttgcggtgg cctttgcggt gtcggacagc
     4141 gggatcgggc tcacgctgac agagatcaag cgactgttcc ggcccttttc gcaggccaac
     4201 gtcacgatcg cctcgcgctt tggtggcgcg ggactcggat tgtcctccgt caagcagatc
     4261 gcgcgcagca tgggcggcga catcgtcgtg acgccgcggc ggggcggcgg caccaccttc
     4321 acgctcaccg tggcactggc gggcgcggag atggcggacc ggtcgcgtcc ggggacggga
     4381 gtgcgccgcc cgccccggcg gttctccgcc atgcgcatcc tcagcgtcga ggacaatccg
     4441 ttcggccgcg tggtgttgaa cacgatcctg agcgaactcg gccatcaggc ggagttcatc
     4501 gggcgcggcg aggacgccac cgaacggatc gcacaaggcg cgttcgatgc ggttctgatg
     4561 gatatggtgt tgccgggtat cgacggaatc gaggcgatcc ggcggatccg cgcgcttccc
     4621 ccgccgctcg gccgcatcgc gatcatcgga gtgtcgggcc gcagcgacga cgaggcggca
     4681 tcacgtgccg ccggcgccga cgcgttcctg ctcaagcctg tgtctccgcg ggctctagcg
     4741 actgcgctgc ttgaagcgac acgccgttcg gcaggcgcga cttgatgatc gcagcgttca
     4801 actcgccgcc atacacgaag atcgccgcga taaaatacag gaacaccagc gcgatgatca
     4861 ccgacgcaag ccccgcatac atcgtcacgt aattgttcgc gaagcgcgtg agatattggc
     4921 cgaacacgat gcccgcgatc agcgaggcga ccagcgtgaa gacgatgccc ggcaagatct
     4981 gcaggaagct gcggcggccc gccggaaccc aggcgtgcag gatgaacagc gccacgatca
     5041 tggctgcgat ggtgatgccg tagcgcgaga aatggaaaaa gctccggttg gcttcgacga
     5101 ccaacggcac atagctgcgc atcgcctcca tgatgagcgg tccgagtatg atcaggaacg
     5161 ccatcgccag cgccgtgacg gcggcaacca gggtgtaagc gatcgattcc agccgtagcc
     5221 agtaccagcg tcgcgtctcg accaccgcat aggcgcgatt gagcgcgacg cgcagtgcct
     5281 cgacgccgtt cgaggcgaaa taaacagaga ggaccgcgcc gatggtcagc gcatctgtcc
     5341 gcgtcgtcgt cagcacgtcg tggatttgtc ccgacaatgt cgcggcgacc tgtttcggcc
     5401 aggtctgcag gagcagcgcc gcggcctcgt cggccagttc cttcgagccg aaaaagccgg
     5461 ccagcgaggt cagcacgatc aggaacggaa acattgccat cagcgtcgac agcgcgatgt
     5521 ggcttgcgat cgcccagccg tcgtcggcga ggaaggtgta gaacgcatcc aggccgatat
     5581 tgaagaggta acggaagagc ctcacatttc acccttaccg gcagaagagc gttttgagcc
     5641 agtgccccga tttcggcggc att
//
LOCUS       DATLJT010001706         1846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3865073,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001706 DATLJT010000000
VERSION     DATLJT010001706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865073"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..185
                     /locus_tag="VM910_38890"
                     /pseudo
     CDS             1..185
                     /locus_tag="VM910_38890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611445.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            178..513
                     /locus_tag="VM910_38895"
                     /pseudo
     CDS             178..513
                     /locus_tag="VM910_38895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            725..1798
                     /locus_tag="VM910_38900"
     CDS             725..1798
                     /locus_tag="VM910_38900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HVI68500.1"
                     /translation="MGTDGVRHGFWSHCLAAGAVATALALAPHLASADEVGESFWTPG
                     SFGSLAATASQPGFSLSSTYYHTSTTAGSEVARARLVRIGRAAVDENVSAISISPEDL
                     AMVTPSYTFATPVLGAQAAIALTATYGRKRTTSDAIITTAPRDGPFATTRFDTISDVV
                     TGFGDLSPQISLRWNAGVHNVMTYLAGNIPVGAYDRARLSNIGIGHGALDGGAGYTYF
                     DEKTGYEFSAVAGLTYNFINPSTDYQSGVDVHLDWGASRFLTKQLQIGLVGYLYNQAS
                     CDGGSGDRVGCFQSRVAGAGGQLGYTIPMGELEGNVNVRAYKEFAAANRPEGWNVWLT
                     YTLSPAEPTPARSAAPTRRMPTK"
BASE COUNT          352 a          568 c          593 g          333 t
ORIGIN      
        1 ggacatttgc cgtaaagctg ggatcagcca ggcgacctac ttcaattgga agaagaacga
       61 tgggctgctg ccgacggaga tgcggcgaca gacagcttga ggacgagagc gcgacgcttc
      121 ggaaggtggt tgccggtccg agcctcgaca aagagatgct gcaggacgct ttacgccgct
      181 atgcagcttc acctcgatga gatcgcaacc aaaatcacgc cgggcgctca cgctattctc
      241 atcctcgatc aagccgggtg gcatggcgcc aaggccctca aggttccgag caacatctcg
      301 ctcttgcagc tcccgccacg cgcgcccgaa ctcaacggcc aagaagatat ctggcaattc
      361 atgcggcaga actggttgtc gaaccgaatt ttcaaatcct tcgacgacat cgtcgatcac
      421 tgctgctacg cctggaacac cctcatcgat cagccgtgga aaatcatgtc catcgcccgc
      481 cgcgattggg cagccgcagg tcactcaatt tgaggattag tattagtccg aaatgcggac
      541 ttctgaacca aagccactag atcaacgagt tgctagtgtc cttcgattcc gaagttcgca
      601 aatgaggctg cggcacaatg atgcgaactt cggaatcggg acactagcgc gcaaggctct
      661 ccgtatagtt ggagcgcgga tgccggttga ctgagtgact gccgcgagcg cctttggggg
      721 caaaatgggg acggacggtg tgcggcacgg cttttggtcg cactgcctgg cggccggggc
      781 cgttgcgacg gcgctcgcgc ttgcgccgca ccttgcgtcg gcggatgaag tcggggagag
      841 cttctggacg cccggatcgt tcgggagcct cgccgccacg gcgtcgcagc ccggcttctc
      901 cctgtcgtcg acctattacc acacgtcgac gacggccggc agcgaggtcg cgcgcgcccg
      961 cttggtcagg attggacggg ctgcggtcga tgaaaacgtg agcgcaatct cgatctcacc
     1021 cgaagatctg gcgatggtca cgccgtcgta cacgttcgcg acgccggttc tcggcgctca
     1081 ggcggccatc gcgctgacgg ccacttacgg ccgcaagcgg acaaccagcg atgcgatcat
     1141 cacgaccgcg ccgcgggacg ggccgttcgc gacaactcgg ttcgacacca tcagcgacgt
     1201 ggtgacgggc ttcggggacc tgtctccgca gatctcgctg cgctggaatg ccggcgtgca
     1261 caacgtcatg acctacctgg ccggcaatat tccggtcggc gcctacgatc gggcccgcct
     1321 ctcgaatatc ggcatcgggc acggcgcgtt ggacggcggg gccggctaca cctatttcga
     1381 tgagaagacg gggtatgaat tttcggcggt ggccggactg acttacaact tcatcaatcc
     1441 ctccaccgac tatcagagcg gcgtcgacgt ccatctggac tggggcgcgt cgcggttcct
     1501 gaccaagcag ctccagatcg gcttggtggg ctatctctac aatcaagcga gctgcgatgg
     1561 cgggtccggc gatcgggtcg gttgctttca gtcgcgcgtt gcgggcgccg gtgggcagct
     1621 cggttacacc atccccatgg gcgagctcga gggaaacgtg aacgtcaggg cgtataagga
     1681 gttcgccgcc gcgaaccggc cggaaggctg gaacgtgtgg ctcacctata cgctgtcgcc
     1741 cgccgagcct acgccggcgc ggtcggcggc gccgacgcgg cgcatgccca cgaagtagcg
     1801 gagctgcgac accacgacac ttgggggccg cgggcgcaag cgggct
//
LOCUS       DATLJT010001707         1656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2943632,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001707 DATLJT010000000
VERSION     DATLJT010001707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943632"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VM910_38905"
     CDS             complement(<1..1102)
                     /locus_tag="VM910_38905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HVI68501.1"
                     /translation="MLSRVTNVIDRRSSNRVLTRLRALLRSNEFYLIPLALVVGIMAG
                     AIVTLMSEIAQIAHVLIFGIPIDVRLSANAYVNPVVALIAPAAGGLLLGIMEWSRRRM
                     KISSAVDPIEANALRGGRLSLRDSVVVSGQTLISNGCGASVGLEAGYTQIGSGAASLL
                     GQVLNLRRNDLRLIVGCGAAAAIAAAFGAPITGAFYACELIVGVYSVGSAAPILAASL
                     AGALTAQYLGGAPYSLEVPRVSSVGLEQYLALIVLSLITAGIGIAVMRSSSLFERLFA
                     QSWLPLWIRPAIGGVCVGAMAIITPQVLAAGHGAMVLNLHREMATGFIATVIVLKLGA
                     CLISLASGFRGGLFFASLFVGSMIGKLFAALLA"
     gene            complement(1125..>1656)
                     /locus_tag="VM910_38910"
     CDS             complement(1125..>1656)
                     /locus_tag="VM910_38910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVI68502.1"
                     /translation="LLITPTSVPYDGMSPDQIVFMGMDGSVAAGHRPSSEWRFHLDIL
                     RARQEVEAVVHAHPTYCTILAIMGLEIPPIHYMIAAAGGDSIRCAPYATYGTPELSQH
                     AVTALEGRLACLLAHHGMIAVGSSLAKALWLAVEVETLARQYHGCLQIGRPPLLSAEE
                     IERVRVKMAGYGHSDK"
BASE COUNT          335 a          534 c          532 g          255 t
ORIGIN      
        1 tcgcaagcaa cgccgcgaac agcttgccga tcatgcttcc gacgaacagc gacgcgaaga
       61 acaggccgcc gcgaaagccc gaggccagag agatcaggca ggcgcccagc ttcaagacga
      121 tgacggtcgc gatgaagccc gtcgccattt cgcggtgcag attgagcacc attgcgccgt
      181 gacctgcggc aagaacctgc ggggtgatga ttgccattgc gccgacgcag acaccgccga
      241 ttgcaggcct gatccacaac ggcagccacg actgcgcaaa cagacgttcg aacagcgacg
      301 agcttcgcat caccgcgatt ccgatcccgg ccgtgatcag agagagcacg atgagcgcca
      361 gatattgttc gaggccgacg gagctgacac gtggtacctc gagcgagtat ggtgcgccgc
      421 cgagatattg cgcggtcaag gcgcccgcca gcgaggcagc gaggatcggc gccgcgctgc
      481 cgaccgagta gacgccgacg atcagttcgc aggcatagaa ggcgcccgtg atcggggcgc
      541 cgaaagcggc cgcgatcgca gcggcggcac cgcagccgac gatcagccgc aaatcgttgc
      601 gccgcaggtt gagcacctgg ccgagcagcg acgcggcgcc cgagccgatt tgggtgtagc
      661 cggcctcgag cccgacggac gcgccgcagc cgttcgagat cagggtctgt ccgctcacga
      721 ccacgctgtc gcgcaacgac aaccggcctc cgcgcagcgc gttggcctcg atcgggtcga
      781 ctgcgctgga gatcttcatg cggcggcgcg accattccat gataccgagc aacaggccgc
      841 cggccgccgg cgcgatcagg gccacgaccg gattgacata agcgttcgcg ctgaggcgaa
      901 cgtcgatcgg gatgccgaag atcaggacat gggcgatctg ggcgatctcg ctcatcagcg
      961 tcacgatggc gccggccatg ataccgacca cgagtgccag cggtatcaga tagaactcgt
     1021 tgctgcgcaa cagcgcgcgc aaccgggtca aaaccctgtt cgagctgcgt cgatcgataa
     1081 cgttcgtcac ccgagaaagc acgtgtcagt cccgcctgtc ctcgtcactt atcgctgtgt
     1141 ccatagcccg ccatcttgac gcggacccgt tcgatctctt ccgcggaaag cagcggaggt
     1201 ctgccgattt gcaggcagcc atgatattgg cgcgccagcg tttcgacctc gacggcgagc
     1261 cacagcgcct ttgcaagcga ggacccgacc gcgatcatgc cgtgatgggc gagcagacag
     1321 gccaatcggc cttcgagcgc tgtcacggca tgctgcgaca attccggcgt gccataggtc
     1381 gcgtagggcg cgcaacgaat gctgtcgccg ccggcggccg cgatcatgta atggatcggt
     1441 ggaatctcca gtcccatgat tgcgagaatc gtgcagtagg tcggatgcgc atgcacgacc
     1501 gcctcgacct cctgccgcgc gcgcaggata tccaggtgaa atcgccattc gctcgacggc
     1561 cgatggccgg ccgcgacgga accatccata cccataaaga cgatttgatc cgggctcatc
     1621 ccgtcgtagg gcacgctggt gggggtgatc agcagg
//
LOCUS       DATLJT010001708         1530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1104047,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001708 DATLJT010000000
VERSION     DATLJT010001708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104047"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VM910_38915"
     CDS             <1..417
                     /locus_tag="VM910_38915"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI68503.1"
                     /translation="SQERMDYTIIGRAVNVAARLESAARPSTILIAHETWTLVKDAII
                     AKEVPPLSVKGLADPLHAYEVLDLREMLPRRTIRHNIDGVSVMIELGRDDGEQAIHIL
                     KKIIGELDYDRTFESAWSQSKNSPPSVRDFCNSNSS"
     gene            complement(672..1067)
                     /locus_tag="VM910_38920"
     CDS             complement(672..1067)
                     /locus_tag="VM910_38920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003569124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI68504.1"
                     /translation="MEIKQNGTQPSAKGPADWFTGTVRIDPLFKAPHPARVSGSSVTF
                     EPGARTAWHTHPLGQTLIVTAGCGLAQREGERVEQIRPGDVVWFAPGEKHWHGAGPAT
                     AMTHIAIAEALDGKVVDWLEHVSDAQYRA"
     gene            1169..>1530
                     /locus_tag="VM910_38925"
     CDS             1169..>1530
                     /locus_tag="VM910_38925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI68505.1"
                     /translation="MQRGHLDDLLAFLAVAREQSFTKAAAKLGVSQSALSHTMRELEA
                     RLGVRLLTRTTRRVAPTEAGERLVQSLGPRFEEIEAELAALSDLRDKPAGTVRITATE
                     YAADAVLLPKLSKLLRDYP"
BASE COUNT          333 a          465 c          451 g          281 t
ORIGIN      
        1 agccaggagc ggatggacta cacgatcatc gggcgcgcgg tgaacgtggc ggctcggtta
       61 gagtcggctg cacggcctag cacaatccta atcgcgcacg aaacatggac attggtaaaa
      121 gacgcgatca tagccaagga ggttccgcca ctgagcgtca agggcttggc cgacccgctg
      181 catgcctatg aggttttgga tctccgcgaa atgctgccgc gcaggacaat tcgacacaac
      241 attgacggtg tgagtgtgat gatcgaactc gggcgcgacg acggcgaaca agccattcac
      301 atcctcaaaa agatcattgg cgaactcgac tatgacagaa catttgaaag cgcctggtct
      361 cagtcgaaaa actcaccacc cagcgtcaga gacttttgca actcaaattc gagttagtca
      421 tcaacctcaa caccaatgcg ctcggcctta cgatgccacc ggcactcctg acgcgcgccg
      481 acgaggtgat cgaataacaa catgccttgc cgcagcgcgg tatgtccgct tttggcacaa
      541 agcggacatg gcgatcgcgc ttagcgatgt ccgctttcgg gggtaaagcg gacatcgaac
      601 gaacgccctc caatttccgc ttatgaccca aagcggacat cccggccgaa gtcatgagat
      661 tttcgccgcg attaggcgcg atactgcgcg tcactaacgt gttccagcca atcgacaacc
      721 ttgccgtcaa gcgcttcggc gatcgcgatg tgcgtcattg ccgtggccgg tcctgcacca
      781 tgccaatgtt tctcgcccgg cgcgaaccag acaacgtcgc ccggccggat ctgctcaacc
      841 cgctcgccct cacgctgcgc aaggccgcag cctgccgtca cgatcagcgt ctgaccgagc
      901 ggatgcgtgt gccaggcggt gcgcgcgccg ggctcgaagg tgacgcttga gccgctgacg
      961 cgcgccggat ggggcgcctt gaacaacggg tcgatgcgga ccgtaccggt gaaccagtcc
     1021 gccggtccct tcgcagacgg ctgagtgccg ttttgcttga tctccatggc tgtgatcctc
     1081 ggttaggagc tcgaggaatg atgtaggacc acgcggtccg ttcgagtagc cgctataatg
     1141 agcataaact tatgagcgag gaccatgaat gcagcgcggg catctggatg accttctcgc
     1201 cttcctcgcc gtggcgcgcg agcagagctt cacaaaagcc gccgccaaac tcggcgtgtc
     1261 gcaatcggcg ttgagccaca ccatgcggga acttgaggcg cggctcggcg tgaggctcct
     1321 tacccgtaca acacgacgag tggcgccgac agaagcgggc gagcgactgg tgcaaagcct
     1381 tggcccgcga tttgaggaga tcgaggccga gcttgcagca ttgagcgacc ttcgcgacaa
     1441 gcccgccggc accgttcgca ttacagcaac cgaatatgcg gcagacgcgg tgctgttgcc
     1501 gaaattatca aagctgctgc gcgactatcc
//
LOCUS       DATLJT010001709         7084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3636124,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001709 DATLJT010000000
VERSION     DATLJT010001709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3636124"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VM910_38930"
     CDS             <1..869
                     /locus_tag="VM910_38930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVI68506.1"
                     /translation="GGFGVALVKVGTIQPAVERDYASVATDLKREIAAERARTQVAAL
                     RDKMEDERGGGASVIEAAQKLGLSAVTIDAVDRSGLMPNGQPANIPPGLNVVSQAFNS
                     DVGVDNDPISFKGGYVWFDVLGITPPRDRTLDEVKDQVEARWREDQIANRLRTKATEM
                     VQKLEQGGTLADEAASVGSKVETATGLKRESSPAGVPSGLIAAAFRTAKNGFGQTPGS
                     GGTEWIVFRVTDITVPPVDLASDEMKKAKESLSRSLSEEQLAEYVAKLEKDIGTSINE
                     AAFAQATGANNN"
     gene            888..1901
                     /gene="trpD"
                     /locus_tag="VM910_38935"
     CDS             888..1901
                     /gene="trpD"
                     /locus_tag="VM910_38935"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVI68507.1"
                     /translation="MDDFKSIIGKVATGATLSREEAASAFDSMMSGEATPSQMGGLLM
                     ALRVRGETVDEITGAVSAMRSKMLRVTAPPDAVDIVGTGGDGSGSVNVSTCAAFIVAG
                     AGVPVAKHGNRALSSRSGAADVLSSLGVRIDIKPEQVGRCVKEAGIGFMFAPSHHPAM
                     KNVGPTRVELATRTIFNLLGPLSNPAGVKRQMVGVFSRQWVQPLAQVLKNLGAEATWV
                     VHGSDGLDEITLTGPTFVAALEKGEIRTFEVTPEDAGLPRCGNDALKGGDGDANAVAL
                     QSVLDGKTSPYRDVALLNAAAALIVAGRARDMKEGVAIGQKSLDSGAAAARLKRLIVV
                     SNS"
     gene            1915..2733
                     /gene="trpC"
                     /locus_tag="VM910_38940"
     CDS             1915..2733
                     /gene="trpC"
                     /locus_tag="VM910_38940"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HVI68508.1"
                     /translation="MSDILTRIEAYKRDEIAAAKRAHPLSDVEARAKAQSSPRGFVRA
                     IRQKLTSGDYALIAEVKKASPSKGLIRADFDPPALAKAYEAGGAACLSVLTDTPSFQG
                     HLDFMVAARAATGLPVLRKDFMFDTYQVAESRAYGADCILIILASLDDAAASDIEDAS
                     LAYGMDVLIEVHDRAELERALKLRSPMIGVNNRNLRTFETTLATSEALAPLIPKDRLM
                     VGESGIFAPADLARLARVGISTFLVGESLMRQADVTAATRALLERETASTTGMR"
     gene            2733..3230
                     /gene="moaC"
                     /locus_tag="VM910_38945"
     CDS             2733..3230
                     /gene="moaC"
                     /locus_tag="VM910_38945"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVI68509.1"
                     /translation="MARKSSKSGPALTHIDAGGAARMVDVSEKPATDRTAVAEGRVIM
                     TKATLDMILSGNAKKGDVFGAARIAGIMAAKRAHELIPLCHPLALAKVALDVTPDRKL
                     PGCVVRATVKVTGPTGVEMEALTAVSVACLTIYDMIKAAERGVRIEGIHLVEKIGGKS
                     GHYRA"
     gene            3341..3601
                     /locus_tag="VM910_38950"
     CDS             3341..3601
                     /locus_tag="VM910_38950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68510.1"
                     /translation="MSTLKLFAAAVLASVVATPVLAQAVISEPGYCAFFYPTANCNNK
                     GPGNPYTDPNWRRDGGGWSAGQVPNSTVGVVRKRPPRHRSES"
     gene            complement(3712..5049)
                     /locus_tag="VM910_38955"
     CDS             complement(3712..5049)
                     /locus_tag="VM910_38955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVI68511.1"
                     /translation="MRKNFLFLLGSLTGACLTLLVSGPQGEHLIAKAKAAANATDTYS
                     QLNLFGEVFERVRADYVEKPDDAGLMEGAINGMVTSLDPHSRYMNDKAWREMQETTSG
                     EFGGLGIEVTMEDGLVKVVAPIDDTPAAKAGILSGDLISQIDDEPVQGLTLEQAVNKM
                     KGSVSTKTRLKILRKGKEAPIDVVLTREIIRIRPVRYHTDGGDIGYIRITSFNEQTTD
                     ALHKAIHDIAKEVPQDKLVGYVVDLRNNPGGLLDQAVSVSSTFLARGEVVSTRGRNPE
                     ETQRFTAHGGDLSKGKPLVVLINGGSASASEIVAGALHDHKRATLIGTRSFGKGSVQT
                     IIPLGVGNGALALTTARYFTPSGHSIQAQGIKPDIDVLQEVPDELKGRADIKGEASMR
                     GHLSAEGAEQSGSQSYVPPDEKDDKALIAAYNLLRGVTVNADVRPPPKAAVPN"
     gene            complement(5109..6293)
                     /locus_tag="VM910_38960"
     CDS             complement(5109..6293)
                     /locus_tag="VM910_38960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HVI68512.1"
                     /translation="MSETLLFTPLTLRDVTLRNRIVVPPMHQYSAVKGFPTDWHLMNA
                     GKFAAGGAGLVVVESTKVERRGCGTVGDLGIWDDVFIEPLQRLVAFIKQQNAAAGIQL
                     GHSGRKARANRPWEGDGPLLRTPDIHDWEAWTPVAPSAIAHSEKWPVPRALERHEVKD
                     LVEAWGQGARRAHQAGFDMLELHGAHGYLVHEFLSERSNQRTDEYGGSESNRMRFIIE
                     IAEAVRAHWPDRKPLFVRLSVEDHAGWGPEQSARLAKILKAKGVDVIDCSSGGITDAA
                     PILGKEIKYSYQVPLSEYVRRHADIMTMAVGLIIHGDQAEQILREGQADLIAVGREIL
                     NNPNWPMDAALKLGVEGPFRNVPPQFGYWLGARAKRGFGTRPSTWQAGLDIGLEETRK
                     VL"
     gene            6476..>7084
                     /locus_tag="VM910_38965"
     CDS             6476..>7084
                     /locus_tag="VM910_38965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HVI68513.1"
                     /translation="MNEAIRPARNVELGASNEPFQNIHEFVRKARANLNQNAWDYIVG
                     AAETETTMRRNRMSLDEIAFRPRVLRNVAKVDASVERFGRKLRLPVMLAPVGALEIFD
                     PNGAAAVVRGVGSFGAAHMLSSVSQPGLEKTADAAPNALRFFQLYTRGDDAFVEDHVS
                     RAIAKGYAAFCLTVDTAHYSRRERDIAKRYVRESRLRATGGDY"
BASE COUNT         1310 a         2259 c         2245 g         1270 t
ORIGIN      
        1 aaggcggttt cggcgtcgcg ctcgtcaagg tcggcacgat ccaaccggcg gtcgagcgcg
       61 attacgcgag cgtggccacc gacctgaagc gcgaaatcgc ggccgagcgc gcccgaaccc
      121 aggttgccgc actgcgcgac aagatggagg acgaacgcgg cggtggcgcc agcgtgatcg
      181 aagcggcgca gaaattgggg ctgtcggccg ttacgatcga tgccgtcgat cgttcgggac
      241 tgatgccgaa cgggcagccc gccaacattc cgccgggtct caacgtcgtg tcgcaggctt
      301 tcaacagcga tgtcggcgtc gacaacgacc cgatctcgtt caagggcggt tacgtctggt
      361 tcgacgtgct tggcatcacc ccgccgcgcg atcgtaccct cgacgaggtc aaggatcagg
      421 tcgaggcgcg ctggcgcgag gaccagatcg caaaccggtt gcgcacgaaa gccaccgaga
      481 tggtgcaaaa gctcgagcag ggcggcacgc tcgcggacga ggcggcaagc gtcggctcaa
      541 aggtcgaaac agcgaccggt ctcaagcgcg agtcatcacc cgcaggggtt ccctcgggcc
      601 tgatcgctgc ggcgtttcga accgccaaga atggttttgg tcagacaccg ggctccggtg
      661 gtactgaatg gatcgtcttc cgcgtgaccg acattactgt gcccccggtc gacctcgcct
      721 ccgacgagat gaagaaggcg aaggagagcc tcagccgcag cttgagcgag gagcagctcg
      781 ccgaatacgt tgccaagctc gagaaggaca tcggcacatc gatcaacgag gccgccttcg
      841 cccaggcgac gggtgcaaac aataattgag gattttgaaa gcacgcgatg gacgatttca
      901 aatcgatcat tggcaaagtc gcaaccggcg cgacgctgtc gcgcgaggaa gccgcttccg
      961 ccttcgacag catgatgtcg ggggaagcga cgccctcgca gatgggaggc ctcctgatgg
     1021 cgctcagggt ccgcggcgaa accgtcgacg agatcaccgg cgcggtctcc gcgatgcgct
     1081 cgaagatgct gcgggtgacg gcgccgcccg atgccgttga tatcgtcgga accggcggcg
     1141 acggctccgg ttcggtgaac gtctcgacct gcgcggcctt tatcgtggcg ggtgccggcg
     1201 ttcccgtcgc caagcacggc aaccgggccc tctcgtcacg ctcgggcgct gccgatgtgt
     1261 tgtcctcgct cggggtcaga atcgacatca agcccgaaca ggtcggccgc tgcgtcaagg
     1321 aggccgggat cggcttcatg ttcgctccct cccatcatcc cgcaatgaag aacgtagggc
     1381 cgacccgcgt cgaactcgcc acccgcacga tcttcaacct gcttgggccg ctttccaatc
     1441 cggccggcgt caagcggcag atggtcggcg tgttctcccg gcagtgggtg caaccactcg
     1501 cgcaggtttt gaaaaatctt ggagccgaag cgacctgggt ggtgcacggc tcggacgggc
     1561 tcgacgaaat caccctcacc ggcccgacct tcgtggccgc gctcgaaaag ggcgagatcc
     1621 gcaccttcga ggtcacgccg gaagacgcgg gcctgccccg ctgcggcaac gacgccttga
     1681 agggcggcga cggcgatgcc aatgcggtcg cgctgcaatc ggtgcttgac ggcaagacga
     1741 gcccctatcg cgatgtcgcg ctgctgaatg ctgccgcagc cctgatcgtc gccggccgtg
     1801 ccagggatat gaaggaaggc gtcgcgatcg gccagaagtc gctcgacagc ggtgctgcgg
     1861 cggcccgcct gaagcgcttg atcgtggtct caaatagctg aggcggaagg ctcgatgtcg
     1921 gacatcctga ccaggatcga ggcctacaag cgcgatgaga tcgcggccgc caagcgtgca
     1981 catccgctgt ccgacgtcga ggcccgcgca aaggcacagt cctctccgcg cggcttcgtc
     2041 cgcgccatcc ggcagaagct tacctccggc gactatgcgt tgatcgccga ggtgaagaaa
     2101 gcctcgccct ccaagggatt gatccgcgcc gatttcgatc cgccggcact cgcaaaggct
     2161 tatgaagcgg gtggcgcggc ctgcctgtcg gtgctgacgg acacgccgtc ctttcaggga
     2221 catctcgatt tcatggtcgc ggcgcgggcc gcgaccggct tgccggtgct gcgcaaggat
     2281 ttcatgttcg acacctatca ggtggcggaa tcgcgcgcct atggagccga ctgcatcctg
     2341 atcatcctgg cgtcgctcga tgacgccgcg gcaagcgaca tcgaggatgc gagccttgcc
     2401 tacgggatgg atgtcctgat cgaagtccac gaccgcgccg aactcgaacg cgcgttaaaa
     2461 cttcgctcgc cgatgatcgg cgtcaacaac cgcaacctgc gcaccttcga gacgacgctc
     2521 gcaaccagcg aagcactggc accgctgatt ccgaaagacc gcctgatggt tggcgaaagc
     2581 ggcatctttg cccccgccga tctggcgcgg ctggcgcgtg tcggtatttc aaccttcctg
     2641 gtcggcgaaa gcctgatgcg gcaagcggat gttacggcag ccacgcgcgc gctgcttgag
     2701 cgcgaaactg caagcacgac gggcatgcgc tgatggcgcg caaatcgtcg aagagcggcc
     2761 ctgccctcac ccacatcgat gccggcggcg cagctcgcat ggtcgacgtg tcggaaaagc
     2821 cggcgaccga ccgcaccgcg gtggccgaag gccgcgtgat catgacaaaa gcgacgctcg
     2881 atatgatcct gtcgggcaac gcgaaaaagg gcgacgtgtt cggcgcagca aggatcgccg
     2941 gtatcatggc ggccaagcgg gcgcatgagc tgatcccgct ctgccatccg ctggcgcttg
     3001 cgaaggtcgc cctcgatgtc actccggaca ggaaactacc gggctgcgtc gtccgtgcga
     3061 cggtcaaagt gaccgggcca acgggcgtag agatggaagc tctcacagcc gtgtcggtcg
     3121 catgcctcac gatctatgac atgatcaagg cggcggagcg cggcgtccgc atcgaaggca
     3181 ttcatctcgt tgaaaagatt ggcggcaagt ccgggcacta ccgggcgtga gggacggccc
     3241 gtgccgtcac ggttgattga tcggcaattt ggaagaatct cccggcttcg gtgttgccta
     3301 acgcgtagtc tccacgcatg acgcgaaagg agcagagcca atgtcgaccc tcaagctctt
     3361 cgccgcggcc gttctcgcga gcgtcgtcgc aacaccggtg ctggcgcagg cggtgatttc
     3421 cgagcccggc tactgcgcat tcttctaccc tactgcgaat tgcaacaaca aaggtccggg
     3481 caacccctac accgatccga actggcgtcg cgacggaggc ggctggagcg cgggccaggt
     3541 gccgaactcg acggttggcg ttgtgcggaa gcgaccaccc aggcaccgtt cggaatcctg
     3601 aagcccccca gatacagccg cgacgatcag cgcaacgagt tgttggtcga cctgaaatat
     3661 tgggcggccg aacggccgcc ctcttcgttg tcaagattca ctcgacttgg ctcaattcgg
     3721 cactgccgct ttcggcggtg gccgtacgtc ggcgttgacg gtgacgccgc gcaacaggtt
     3781 gtaggcggcg atcagcgcct tgtcgtcctt ctcatcgggc ggcacgtacg actgcgagcc
     3841 gctctgttct gcaccctctg ccgagagatg gccccgcatc gaggcttcgc ctttgatgtc
     3901 ggcccgcccc ttgagctcgt cgggcacttc ctgcagcaca tcgatgtcgg gcttgatgcc
     3961 ttgagcctgg atggaatgcc cggacggcgt gaaatagcgc gccgtcgtca atgccagcgc
     4021 gccgttgccg acgccgagcg ggataatggt ctgcaccgaa cccttgccga aggacctcgt
     4081 tccgatcagc gtcgcgcgct tgtgatcgtg gagcgcgccg gccacgattt ctgaagcgga
     4141 cgccgatccg ccgttgatca ggacgaccag gggcttgcct ttgctgagat cgccgccgtg
     4201 cgctgtgaag cgctgggttt cctcggggtt acggcctcgc gtcgagacga cctcgccgcg
     4261 cgccaggaaa gtgctggaga ccgaaaccgc ctgatcgagc aacccgcccg gattgttgcg
     4321 gagatcgacg acatagccca caagcttgtc ctgcgggacc tccttggcga tgtcgtggat
     4381 cgccttgtgc aacgcgtcgg tggtctgctc gttgaacgag gtgatccgaa tgtaaccgat
     4441 gtcgccgccg tcggtgtgat agcgcaccgg gcgtatgcgg atgatctcgc gggtgagcac
     4501 gacgtcgatc ggcgcctctt tgcccttgcg gagaatcttt agccgggtct tggtgctgac
     4561 ggagcccttc atcttgttga cggcctgctc gagggtaagc ccctgcaccg gctcgtcgtc
     4621 gatctggctg atcaggtcgc ccgacaggat gccggccttc gccgccggcg tgtcgtcgat
     4681 gggagcaacg accttgacca ggccgtcttc catcgtgacc tcgatgccaa gtccgccgaa
     4741 ttcgccggac gtcgtctcct gcatttcgcg ccaggccttg tcgttcatgt aacgcgagtg
     4801 cggatcgagc gacgtcacca tgccgttgat cgcaccctcc attaggccgg cgtcgtcagg
     4861 cttctcgacg taatcggcgc ggacgcgctc aaacacctcg ccgaacaggt tgagctggga
     4921 ataggtatcc gttgcattcg ctgcggcttt tgccttggcg atcagatgct cgccctgggg
     4981 accgctaacc agcagggtca gacacgcgcc tgtcagcgaa ccaagtaaaa ataggaaatt
     5041 cttccgcatc tgctccgcag ccttcccgcg attgttctgt gggcccacgt agggtcgaaa
     5101 gtggaagctc acaatacctt tctggtttct tcaaggccga tatccaggcc ggcctgccag
     5161 gtcgacggcc tggtaccaaa gccgcgtttg gcgcgggcgc cgagccaata accgaactgt
     5221 ggcgggacat tacggaaggg tccctcgacg cctaatttca aggcagcatc cattggccag
     5281 ttcggattgt tgaggatttc ccgtccgacc gcgatcaggt cggcctggcc ctcccgcaga
     5341 atctgctcag cctggtcgcc gtggatgatc agtccgaccg ccatggtcat gatatcggca
     5401 tggcgacgaa catattccga caacggcacc tggtaactgt acttgatctc cttgccgagg
     5461 atcggcgcgg catcggtaat gccacccgag gaacaatcga tcacgtcgac gcccttggcc
     5521 ttcaaaattt tcgccaaccg tgcgctctgt tccggtcccc agccggcatg atcttccacc
     5581 gatagccgaa cgaacaacgg cttgcgatcg ggccagtgcg cccgaactgc ttccgcgatt
     5641 tcgatgatga agcgcatccg gttggattcg gagccgccat attcgtcagt gcgctgattg
     5701 gagcgttcgg agagaaactc gtgcacgaga tagccatggg cgccgtgcag ctccagcatg
     5761 tcgaagccgg cctggtgagc ccggcgcgca ccctgtcccc aggcttccac gaggtccttg
     5821 acctcgtgtc gctccagagc cctcggcacc ggccatttct cgctatgagc gatcgcactc
     5881 ggcgcgaccg gcgtccacgc ttcccagtca tggatatcag gcgtgcgcag gagcgggccg
     5941 tctccctccc aggggcgatt ggcgcgcgcc ttgcggccgg aatgaccgag ttgaatgccg
     6001 gccgccgcgt tctgctgctt gatgaatgcg accaaccgct gcagcggctc gatgaaaacg
     6061 tcgtcccaga tgccgaggtc gcccacggtg ccgcagccgc gccgttcgac cttggtcgat
     6121 tcgacgacca caagacccgc gccgccggcg gcaaacttgc cggcgttcat cagatgccaa
     6181 tcggtcggaa accccttcac cgccgaatac tgatgcatcg gcggtacgac gatgcggttg
     6241 cgcaacgtca cgtcccgtag cgtcagcggc gtgaacagca gcgtctccga catggccggt
     6301 ttcctcgtgt aatctgatta tcaaggatat cgggcggtgc gtgcgccgca aagtggcggc
     6361 agggctgcaa acgtcaaatc cattcgacta gccatgcaat tttgggatgg cgtgaagccg
     6421 cgggaaggga taggcgaaag agcaacaaca gccttgcact ccgggggagg acatcatgaa
     6481 cgaagccatc cggccagcac ggaacgtcga actcggcgcc agcaacgaac cgttccagaa
     6541 catccacgag ttcgtccgga aggcccgcgc caacctcaac caaaatgcgt gggactatat
     6601 cgtcggcgcc gccgagaccg agaccaccat gcggcgcaac cgcatgtcgc tggacgagat
     6661 cgcgttccgc ccacgcgtgc tgcgaaatgt ggccaaggtc gacgcctcgg tggagcggtt
     6721 cggccgcaag ctgcggctgc ccgtcatgct ggccccggtc ggcgcgctcg agatctttga
     6781 tcccaatggc gctgctgccg tggtgcgcgg tgtcggcagc tttggcgcgg cgcatatgct
     6841 gagctcggtg tcccagccgg gcctggagaa gaccgcggat gcggctccca atgcgctccg
     6901 cttcttccag ctctatacgc gcggcgacga cgcctttgtc gaagaccatg tcagccgcgc
     6961 gattgcaaaa ggctatgccg cgttctgcct gaccgtcgac accgcccatt acagccgcag
     7021 agagcgcgat atcgccaagc gctacgttcg cgaaagccgc ctgcgcgcca ccggcggcga
     7081 ctat
//
LOCUS       DATLJT010001710         1909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2944482,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001710 DATLJT010000000
VERSION     DATLJT010001710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944482"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VM910_38970"
     CDS             <1..559
                     /locus_tag="VM910_38970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HVI68514.1"
                     /translation="VIEFFRGSSALVQLFWAFYVLPFFGIQLPPLVAGILVLGLNEGS
                     YFSEVVRAGLDAVPKGQREAAVTLGLPFHYRLFRIILPQALPIMVPPFGNELITMLKF
                     TALVSLVTVQDLSFRASLISSTLGESGPIFGLTIIIYFVLALLLGGGVRLLERPVSRW
                     AGRDFSAAPAARRSSIPDWAVGGTR"
     gene            556..1221
                     /gene="ehuD"
                     /locus_tag="VM910_38975"
     CDS             556..1221
                     /gene="ehuD"
                     /locus_tag="VM910_38975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HVI68515.1"
                     /translation="MSSFHWDWGFAWAILPELLRGLVVTVLATLLGSMVAFALGMVWT
                     LVRVLRIPFLTPSVSFFVDFIRGTPLLIQLYFLYYVLPTWGLTISALPTGIFGLGLFY
                     SAYAAEIFRAGIEDLPAGQWEAALTLGLPVRRVWLGIILPQAIRSVVPILGNVVISMF
                     KQSSLLSTITVFELLAAGLNIGSVNYRYVEPLTLVGAFYFAISFLSARIVRSLEAKHA
                     FGD"
     gene            1205..>1909
                     /locus_tag="VM910_38980"
     CDS             1205..>1909
                     /locus_tag="VM910_38980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HVI68516.1"
                     /translation="MPSEIESRTHDPMAATASTPAQALLSMRSITKSYGDHVVLNHID
                     LDVAPREKVAVIGPSGSGKTTILRAAIGLVKPDEGTIRIDGEYLWHVKATDGLRPAGE
                     RHARRVRRAVGIVFQQFNLFPHMTALENVREPLVHALGIRRQESDARATDLLCQVGLK
                     DHLGKHPAQLSGGQQQRVAIARSLALRPKIMLFDEVTSALDPELVGEVLRVVRQLASE
                     GEMSMLIVTHEISFAAD"
BASE COUNT          297 a          591 c          573 g          448 t
ORIGIN      
        1 cgtcatcgag ttcttccgcg ggagctcggc gctcgtccag ttgttctggg cattctacgt
       61 gctgccgttt ttcggcatcc agcttcctcc gttggtcgcc ggcattctcg tcctcggatt
      121 gaacgagggg agctactttt ccgaggtggt ccgagccggt cttgatgcgg ttccgaaagg
      181 ccagcgcgaa gccgcggtta cactgggcct cccgtttcac tatcgcctgt ttcgaataat
      241 cctgccgcag gcacttccga tcatggtgcc gccgttcggg aacgagctga tcaccatgtt
      301 gaaatttacc gcgctggttt cgctggtgac ggtccaggac ctgtcgtttc gcgcgagcct
      361 catcagcagc acgcttggcg aaagcggtcc gatattcggc ctgaccatca tcatttattt
      421 cgtgctggcg cttctgctgg gcggcggcgt ccggctgctt gagcgcccgg tcagccgctg
      481 ggcgggccgt gatttctccg cggctcccgc ggcgcggcgc tcgtccattc ccgattgggc
      541 ggtggggggc acgaggtgag ctcgttccat tgggactggg gcttcgcctg ggcaatcctt
      601 ccggagcttc tacgggggct tgttgtcacc gtgctggcaa ccttgctcgg ctcgatggtt
      661 gcttttgcgt tgggaatggt ctggaccctt gttcgcgtgc tccgcatacc cttcctgacg
      721 ccgtccgttt cattcttcgt cgacttcatt cgcggcaccc cgctgctgat ccagctttac
      781 ttcctgtact atgtgctgcc gacatggggc ctgacgattt ccgccttgcc cacgggcatt
      841 ttcgggctcg ggctgttcta tagcgcctac gcagccgaaa tctttcgcgc cggcatcgag
      901 gaccttcccg ccggtcaatg ggaggcggcg ctcaccctcg gcttgccggt ccgtcgcgtc
      961 tggcttggca tcatcctgcc gcaggccatc agatcggtcg tgccgatttt gggcaatgtc
     1021 gtcatcagca tgttcaagca gagctcgctt ctttcgacca tcacggtatt cgagctgctc
     1081 gccgcaggac tgaatatcgg ttccgtcaat taccgatatg tcgagccgtt gactcttgtc
     1141 ggcgcgtttt atttcgccat cagctttctg tcagctcgga tcgtccgctc cttggaggca
     1201 aagcatgcct tcggagattg aatcgcgcac gcacgatcct atggcggcga cggcgagcac
     1261 gcccgcgcag gcgctgctct caatgcgaag catcacaaag agctatggcg atcacgtggt
     1321 cctcaaccac atcgatctcg acgtggcgcc gcgcgagaaa gtcgcggtta tcgggccgag
     1381 cggatccggc aaaaccacga ttctgcgcgc ggccataggt ctcgtaaagc cggacgaagg
     1441 caccatccgg atcgatggtg agtatctttg gcatgtcaag gccaccgacg gtctgcgacc
     1501 cgccggcgag cggcatgccc gcagggtccg tcgcgcggtt ggcatagtgt ttcagcaatt
     1561 caatcttttt ccgcacatga cggcgctgga gaatgtccgc gagccgctcg ttcacgcgct
     1621 agggatacgg cggcaggaga gcgatgcaag agccaccgat cttttgtgcc aagttggtct
     1681 caaggatcat ctcggcaagc atccggcaca gctgtccggg ggtcaacagc aacgcgtcgc
     1741 cattgcgcgg tcgcttgccc tcaggccgaa gatcatgttg ttcgacgaag tcacctccgc
     1801 tctcgacccg gagctggtcg gcgaggtcct gcgcgtcgtg cgtcagctcg cctccgaggg
     1861 cgaaatgtca atgcttatcg tcacccatga gattagcttt gccgccgac
//
LOCUS       DATLJT010001711         4280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_186790,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001711 DATLJT010000000
VERSION     DATLJT010001711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186790"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VM910_38985"
     CDS             complement(<1..1037)
                     /locus_tag="VM910_38985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68517.1"
                     /translation="MRKGLFHLVAGAALAITLSASPAFAQKKYDTGASDTEIKIGQTV
                     PFSGPASAYASIGKTQAAYIQMINDQGGINGRKITLIQYDDAYSPPKAVEQVRKLVES
                     DEVLLTFQIIGTPSNAAVQKYLNAKKVPQLYAATGASRFTDPKNFPWTMGFNPNYFVE
                     GRIYGQYILKNYPNAKVGILYQNDDLGKDYLNGIKAGLGDKAASMVVAEASYEVSDPT
                     IDSQILKIKDAGADLFFSASTPKFAAQAIKKNFELNWHPVHILDINATSVGATLKPAG
                     LEASTGVISVNYGKDPADPTWKDDPGMKRYLDFMTKYYPDGDKGSLFNTYGYSTAQLL
                     VYTLKQCGDDLT"
     gene            1246..1788
                     /locus_tag="VM910_38990"
     CDS             1246..1788
                     /locus_tag="VM910_38990"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HVI68518.1"
                     /translation="MAANTIGTTAATARAAFALDRLKTPIGTALLVTDRDGLLRALDW
                     EDHARRMKELLRLHYGAVMLSDARAPREIRVALSGYFEGEFHRLETIGWRVAGTPFQR
                     KVWSALPAIPPGRTMSYGALAARLNVPKAARAVGHANGANPISIVIPCHRLIGADGSL
                     VKYGGGLSRKRWLLQHEGAL"
     gene            complement(1811..>4280)
                     /locus_tag="VM910_38995"
     CDS             complement(1811..>4280)
                     /locus_tag="VM910_38995"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HVI68519.1"
                     /translation="TGGYGRGLMAPESDIDLLFILPYKQTAWGEQVAEAILYCLWDMG
                     LKVGHATRSVDESIRQARGDMTIRTAILETRFLTGDKPLYDELVARFDKEVVQGSAAE
                     FVTAKLAEREERHRRAGQSRYLVEPNVKDGKGGLRDLHTLFWIAKYVYRVRETDELLE
                     RGVFDAQEYRTFRRCADFLWSVRCNLHFITGRAEERLSFDLQREIAVQLGYTSHPGMQ
                     DVERFMKHYFLVAKDVGDLTAILCAKLEDQQAKPAPVLSRFMARLRPSMHRRRVPESD
                     DFIVDNNRINLAAPDVFKHDPVNLIRIFRLAQKNNLAFHPDAMRNVTRSLKLINTELR
                     ENPEANRLFMEILTSNDAETVLRRMNETGVLGHFIRAFGKIVSMMQFNMYHHYTVDEH
                     LIRCIGFLQEIERGSNDELVLAADLMRKIRPEHRPVIYVAVLLHDIAKGRPEDHSIAG
                     AKVARRLCPRLGFSAADTELVAWLIEVHLTMSTVAQSRDLSDRRTIENFASVVQSVEQ
                     MKLLTILTTADIRGVGPGVWNGWKAQLLRTLYYETEPVLTGGFSEVNRARRIAAAQAE
                     FRATFTEWPEAELAAYIARHYPAYWLKVELPRKIRQARFIRASEQAGHKLAINVGFDE
                     ARSVTELTIFVADHPWLLSIIAGACASAGANIVDAQIYTTTDGRALDTIAISREYERD
                     EDEARRATRIGEMIEDVLEGKLRLPEAVARRAAGRGGRVRPFVVEPEVTINNQWSELY
                     TVIEVSGLDRPGLLYQLTTALSKLNLNIASAHVATFGERARDVFYVTDLLGAQINAPT
                     RQAAIRSALLHLLSSEEAAEKPAA"
BASE COUNT          731 a         1294 c         1422 g          833 t
ORIGIN      
        1 gtcaggtcgt cgccgcactg tttcaacgtg tagaccagaa gctgcgccgt cgagtagccg
       61 taggtgttga acagcgagcc cttgtcgccg tccggatagt acttcgtcat gaagtcgaga
      121 tagcgcttca tgccgggatc gtccttccat gttggatcgg ccggatcctt gccgtagttg
      181 acgctgatca cgcccgtcga cgcctcaagc cccgccggct tcaacgtcgc gccgaccgag
      241 gtcgcgttga tgtcgaggat gtgcacgggg tgccagttga gctcaaagtt cttcttgatc
      301 gcctgcgccg cgaatttcgg cgtggaggcg ctgaagaaca gatcggcacc ggcgtccttg
      361 atcttcagga tctgcgaatc gatggtcgga tcggaaacct cgtaggaggc ttccgccacc
      421 accatgctcg cggccttgtc gccaaggccc gccttgatgc cgttcaaata gtccttaccc
      481 agatcgtcgt tctggtagag gatgccgacc ttggcgttgg gataattttt caggatgtat
      541 tggccgtaga tgcggccctc gacgaagtaa ttcggattga agcccatcgt ccaggggaag
      601 ttcttcggat cggtgaagcg cgacgcgccg gttgccgcat agagctgcgg caccttcttg
      661 gcgttgagat atttctgaac cgcggcgttc gacggtgtgc cgatgatctg gaaggtgagc
      721 agcacctcgt cgctctcgac gagcttgcgc acctgttcga ccgccttcgg cgggctgtag
      781 gcgtcgtcat actggatcag cgtgatcttg cgaccgttga tcccaccctg gtcgttgatc
      841 atctggatat aggcagcctg ggtcttgccg atcgaggcgt aggcggaggc cggcccggag
      901 aacggcacgg tctggccgat cttgatttcg gtatctgacg cgccggtgtc gtatttcttc
      961 tgcgcgaagg ccggcgatgc cgacagcgta atcgccagcg ctgcaccggc gacaagatga
     1021 aaaagccctt tcctcatgtc gtagcgttcc tcttgtgatt ttgtgtgttg ggatttttgt
     1081 cggtgtctcg ccaccgcggc gcgatactgg aggaagcaat accgcgatgc aagatgatac
     1141 gggacggaaa ggcgaatttc cgccgacacg cgtcccggct ttcgggtggc ggctcgcgca
     1201 tggcgaaagt aagaagatac catgttgctc cggagaccgg cgacgatggc agccaacaca
     1261 attggcacga cagccgcaac agcgcgcgca gccttcgcgc tcgaccgcct gaaaaccccg
     1321 atcggcacgg cgctgctcgt gacggaccgg gatggtctgt tgcgtgcgct cgactgggag
     1381 gatcacgcaa ggcgcatgaa ggagctgctg cggctgcatt atggtgcggt gatgctctcc
     1441 gacgcccgcg ccccgcgcga gattcgagtc gcgctgtcgg gatatttcga gggcgagttc
     1501 catcggcttg aaacgatcgg atggcgcgtt gcgggcacgc ctttccagcg caaggtgtgg
     1561 tcggcgttgc cggcgattcc accgggcagg accatgagct atggcgcgct ggcggcgcgt
     1621 ctcaacgtgc cgaaggcggc gcgtgcagtc ggccacgcca acggcgccaa tccgatcagc
     1681 atcgtcattc cctgccatcg cctgatcggc gccgacggct cgctggtcaa atatggtggc
     1741 gggctcagcc gcaaacgctg gctgctgcag cacgaaggcg cgctttgacc ggggacgtat
     1801 cgtgccgcgc tcaggccgcg ggcttttccg ccgcctcttc ggaggacaga agatgcaaga
     1861 gcgcgcttcg gatcgcggcc tgccgggtcg gcgcgttgat ctgcgccccg agcaggtcgg
     1921 tgacatagaa cacgtcgcgc gcgcgttcgc cgaaggtcgc gacgtgggcg gaggcgatgt
     1981 tgagattgag tttcgagagc gcggtcgtca gttgatagag cagacccggc cggtcgaggc
     2041 cggacacctc gatgacggta taaagctccg accattggtt gttgatggtc acttcgggct
     2101 cgaccacgaa cggacgcacc cgtccgccgc gaccggccgc gcggcgcgcc acggcttccg
     2161 gcaggcgcag cttgccttcc agcacgtcct cgatcatttc gccgatccgc gtggcgcggc
     2221 gcgcttcgtc ctcgtcgcgc tcatattccc gcgagatcgc gatggtatcg agcgcgcgcc
     2281 cgtcggtcgt ggtgtagatc tgcgcgtcga cgatattggc gccggccgag gcgcaggcgc
     2341 cggcgatgat cgagagcagc cagggatggt cggccacgaa gatcgtaagc tcggtgacgc
     2401 tgcgcgcctc gtcgaagccg acattgatcg caagcttgtg ccccgcctgt tcgcttgcgc
     2461 ggatgaagcg ggcctgacgg atcttgcgcg gcagctcgac cttgagccaa taggccggat
     2521 aatgccgcgc gatataggcg gcgagctcgg cctcgggcca ctcggtgaac gtcgcgcgga
     2581 actcggcctg cgccgcggcg atgcggcggg cgcggttcac ttccgagaag ccgccggtca
     2641 ggaccggctc ggtctcgtaa tagagcgtgc gcaagagctg cgctttccag ccgttccaca
     2701 cgccggggcc gacgccgcga atgtcggcgg tggtgaggat cgtgagcagc ttcatttgct
     2761 cgaccgactg cacgacgctc gcgaaattct cgatggtgcg gcggtcggag aggtcgcgtg
     2821 actgcgcgac cgtcgacatg gtcagatgca cctcgatcag ccaggcgacg agctcggtgt
     2881 cggccgcgga gaagccgagc cgcggacata gccgccgcgc taccttcgcg cccgcgatcg
     2941 aatggtcttc gggccggccc ttggcgatat cgtgcaggag cacggcgaca tagatcacgg
     3001 ggcgatgttc ggggcggatc ttgcgcatca ggtcggccgc cagcaccagc tcgtcattgc
     3061 tgccgcgctc gatctcctgc aggaaaccga tgcagcggat cagatgctcg tccaccgtgt
     3121 agtggtggta catgttgaac tgcatcatcg acacgatctt gccaaaggcg cggatgaaat
     3181 gaccgagcac gccggtctcg ttcatccgac gcagcacggt ctcggcgtcg ttggaggtga
     3241 ggatctccat gaacagccgg ttggcctccg gattctcgcg cagctccgta ttgatcagct
     3301 tcagcgagcg cgtcacgttg cgcatggcgt cgggatgaaa ggcgagattg ttcttctgcg
     3361 cgagccggaa gatccggatc aggttgaccg gatcgtgctt gaacacgtcg ggtgccgcga
     3421 gattgatgcg gttgttgtcg acgatgaaat cgtcgctctc cggcacgcgc cgccggtgca
     3481 tgctgggccg aagccgcgcc atgaagcggc tcaacaccgg cgcgggcttc gcctgctggt
     3541 cctccagctt ggcacagagg atcgcggtga ggtcgccgac gtcttttgcg accaggaagt
     3601 agtgcttcat gaagcgctcg acgtcctgca tgccgggatg gctggtatag ccgagctgga
     3661 cggcgatctc gcgctgcagg tcgaacgata gccgctcctc ggcgcggccg gtgataaagt
     3721 gcaggttgca acggaccgac cacaggaaat cggcgcatcg gcggaaagtg cgatattcct
     3781 gcgcgtcgaa tacgccgcgc tccaggagct cgtccgtctc gcgcacgcga tagacatatt
     3841 tcgcgatcca gaacagcgtg tgcaggtcgc gcaggccgcc tttgccgtcc ttgacgttgg
     3901 gttcgaccag ataacgtgat tggcccgcgc ggcgatgtcg ttcctcgcgc tcggcgagtt
     3961 tcgcggtcac gaactcggcg gcggaaccct gcacgacttc cttgtcgaag cgtgcgacca
     4021 gttcgtcgta tagcggcttg tcgccggtca gaaaccgcgt ctccaggatc gcggtgcgga
     4081 tcgtcatgtc gccgcgcgcc tggcgaatcg attcatcaac cgagcgcgtg gcatggccta
     4141 ccttcaagcc catgtcccac aggcaataga ggatcgcctc ggccacctgc tcgccccagg
     4201 cggtctgctt gtagggcagg atgaacaaca gatcgatgtc ggattcgggc gccatcaggc
     4261 cgcgcccgta gccgccggtc
//
LOCUS       DATLJT010001712         1643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_874596,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001712 DATLJT010000000
VERSION     DATLJT010001712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874596"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1183)
                     /locus_tag="VM910_39000"
     CDS             complement(122..1183)
                     /locus_tag="VM910_39000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI68520.1"
                     /translation="MRFGIFDHLDDGGVPLGRLFEERLQLIEAYDSAGFYGYHLAEHH
                     ATPLGYAPSPGVFLSAVAQRTRQLRFGPMVYLLPLYHPLRLIEEICMLDQMSAGRFLL
                     GVGRGISPIEVGFFGVDFAHGMQQYEEALAVIRLGLTHDELTYRGEFYNFDAVPIVLK
                     PAQLPHPPLWYGVSRPETLSWAAENDVNLVSFLRPMPAMRSLVEQFQAEWAALGKRPD
                     TLPCIGVTRHIVVAETEKEARNIAARAYRPWRRNMEVLWERRGVRFPLQGELADEFDV
                     LQRHGVAFAGTPAGARDYVAAQAEGSGINYFVCDMAFGDITLDEAKRSVELFAQEVMP
                     ALAMPDQVLKGRNHALWDL"
     gene            1398..>1643
                     /locus_tag="VM910_39005"
     CDS             1398..>1643
                     /locus_tag="VM910_39005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI68521.1"
                     /translation="MAATRRLAAILAADVAGYSRLIGSDEQGTLGRLKAIRADVIDPA
                     IAAHHGRIVKTTGDGVLAEFASTVDALRCAGEMQTQMA"
BASE COUNT          297 a          535 c          545 g          266 t
ORIGIN      
        1 cggcgtgttg tggtgctcgg cgagatggta cccgtagaac ccggcgcggt cataggcttc
       61 gatcagcttg agccgcgcct cgaaatgctg gctgagcggc aggccgctgt cgtcaaggtg
      121 gtcaaagatc ccaaagcgca tggtttctcc ctttgagcac ctgatcaggc atggccaagg
      181 ccggcatgac ctcctgcgcg aacagctcga ccgaccgctt ggcctcatcg agggtgatat
      241 cgccgaaggc catgtcgcag acgaagtagt tgatgccgga accctcggcc tgggcggcga
      301 catagtcgcg cgccccggcc ggggtgccgg caaaagcgac accgtggcgc tgcaggacat
      361 cgaattcgtc ggcaagctcg ccctgcaacg ggaaccggac gccgcgccgc tcccacagca
      421 cttccatgtt gcggcgccac ggccggtagg ctcgtgccgc aatgttgcga gcctccttct
      481 cggtctccgc gacgacgatg tggcgcgtca ccccgatgca ggggagtgtg tccggtcgct
      541 tcccgagcgc cgcccattcg gcctggaact gttcgacgag cgagcgcatc gccggcatcg
      601 gccgcaggaa cgagaccagg ttgacatcat tttcggcggc ccacgatagg gtctccggcc
      661 gcgagacgcc gtaccacagc ggcggatgcg ggagttgcgc cggcttcagc acgatcggca
      721 ccgcgtcgaa attgtaaaac tctccgcgat aggtcagctc gtcatgggtc agccccaggc
      781 ggatcaccgc gagcgcctcc tcgtactgct gcatgccatg cgcgaaatcg acgccgaaga
      841 acccgacctc gatcggcgag atgccgcggc cgacgcccag cagaaagcgg cccgcgctca
      901 tttggtcgag catgcagatc tcttcgatca gccgcagcgg gtgatagagc ggcagcagat
      961 ataccatcgg cccgaagcgc agctgccgtg tccgctgcgc aacagcggag aggaagacac
     1021 cgggcgacgg cgcgtagccg agtggtgtcg cgtggtgctc ggcgagatgg tagccgtaaa
     1081 agcccgcgct atcgtaggct tcgatcagct gcagccgctc ctcgaagagc cggccgagag
     1141 gcaccccgcc gtcgtcgagg tggtcgaata tcccaaagcg caaccgtgcc tcccgtcata
     1201 gcttggccga aggatactat cgttccggcc cggccggctc caagcgcgat cgcgccgccg
     1261 atcgccatgc ggcgctcgga cgctcaaccg tgccgagttc gaccgggggc cggccgcgat
     1321 cagcggttaa ttgaagacgc gtgcgcgata tgggcgggtt gggatagagt gaggaggcaa
     1381 cctgagccgt ccttacaatg gccgcaaccc gtcgtttggc cgccattctt gccgccgatg
     1441 tcgcgggcta ctcgcggctg atcggaagcg atgagcaagg taccctgggg cggctcaagg
     1501 caatccgcgc cgacgtgatc gatccggcca tcgccgcgca tcacggccgc atcgtcaaga
     1561 ccaccggcga cggcgtgttg gccgagttcg ccagcactgt cgacgctttg cgctgtgccg
     1621 gcgaaatgca gacgcagatg gcg
//
LOCUS       DATLJT010001713         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1798696,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001713 DATLJT010000000
VERSION     DATLJT010001713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798696"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..865
                     /locus_tag="VM910_39010"
     CDS             326..865
                     /locus_tag="VM910_39010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68522.1"
                     /translation="MANVETLLDALPVHTIAQAKDFVRLHPEEDKYWSSELCFVNVPI
                     KGSKRDTLHLIEEELAMRFLPSARIQRFRLALATKPYDVFFLCQVPSQNLDNAYNASN
                     LKACEQAKTFWTQATSRREEGVEAYKVTFTRDDNPFPEPKWPSQSLAELIEKTFAGRM
                     INSEDHPGLLRLIGARQSS"
     gene            862..>2301
                     /locus_tag="VM910_39015"
     CDS             862..>2301
                     /locus_tag="VM910_39015"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HVI68523.1"
                     /translation="MSKNFGTIVVADFEYEVQPGELPNPLCMVAHVLDENLRHVRTIR
                     MWREELLASTRPPFDTGPDTLFVAYSAWAELTCLMVLGWPFPTHIFDLHTAYLATTNI
                     LLPYNPEEVRNKPRKRLPDACRAYGIGGWEKIDKNTIAEDIGNGRWQRYGQERVFEYC
                     EEDVRMSVRLLRAQLRRHQCGPTILPAADVPRVLWWSNYSAKAVAQIQARGMPIDVAR
                     WNLVRENKQAVIDELLRRFDPSHGSEEPIYTPEGEWSYARFERFLIRSGVTAWPRLES
                     GKLDIDGDAFRMMSHIPGIEALHALRDTLGVIVRAKLPIGHDGRNRPSLFPFCTATGR
                     NAHAKSLFNAHASVRSFMAFSPDAIGVYLDWRTQEVGIAAALSEDRALIEAYHDGDVY
                     HSLALVCGLTDDTDVKRWKRECPDVRQRMKALQLGINYGMGVPSLARGLDRHPIIASN
                     FIERHRRTYPRFWDWRDNEVQVAMLERRIE"
BASE COUNT          463 a          736 c          721 g          381 t
ORIGIN      
        1 attgacgaga tcgttgtcgg cgaacggcac cgccgcgacc tgggcgacat cgccagcctg
       61 gccgccaacg tggccgagct cggcctgctg catccgatag tcatccacgg aacgctcatt
      121 gccggtgagc ggcggcccaa ccgagccgct gatttatcgc ggcgacacct cgccatccgc
      181 tcgccgaaca gagtccgcaa aggaagcccc agatgagcaa acctgaacta gtgccgactg
      241 caagcccact accgaagcga cggattcgat cgccaagccg gaggggtttg acctcgatag
      301 gttcaaatcc aagcgcgccg cggcgatggc gaacgtcgag acgttgctgg acgccttgcc
      361 ggttcacacg atcgcgcagg cgaaggactt cgtccggttg cacccggagg aagataagta
      421 ttggtcgtcg gagctctgtt tcgtgaacgt ccccatcaag ggatcgaaac gggacacgct
      481 gcacctcatc gaggaagagc tcgccatgcg gtttctgccg agcgccagaa tccagcgctt
      541 tcggctggcg ctcgcgacga agccctacga cgtgttcttc ctctgccaag tgccgagcca
      601 gaacctcgac aacgcgtaca acgccagcaa cctcaaagct tgcgagcagg caaagacgtt
      661 ctggacgcag gcgacgagcc ggcgcgagga gggcgtcgaa gcgtacaaag tgaccttcac
      721 gcgcgatgac aacccgttcc cggagccgaa gtggccatca cagtcgctcg cggagctgat
      781 cgagaagacg ttcgccggtc gcatgatcaa cagcgaagac catcccggcc ttctgcgcct
      841 gatcggggcg cggcaatcgt cgtgagcaag aacttcggga cgatcgtcgt cgccgacttc
      901 gagtacgaag tccagcccgg cgaacttccc aaccccctgt gcatggtggc gcacgtgctg
      961 gacgagaacc tccgccacgt gcgcaccatc cgcatgtggc gcgaggaatt attggcctca
     1021 acgcgcccgc cattcgacac cggaccagat actttgtttg ttgcctacag cgcgtgggca
     1081 gagctcacgt gcttgatggt gctgggctgg ccattcccaa cgcacatctt cgatctgcac
     1141 accgcctacc ttgccaccac caacatcttg ctgccgtaca accccgaaga ggtgaggaac
     1201 aaaccgcgca aacgcctgcc cgacgcgtgc cgggcctacg ggatcggggg gtgggaaaag
     1261 atcgacaaga atacgatcgc cgaggacatc ggcaacggac gctggcaacg ctacggtcag
     1321 gaacgcgtct tcgagtactg cgaggaggac gtccgcatgt cggtgcggct gctgcgcgcg
     1381 cagctgcggc gccaccaatg cggtcccacg atattgccgg cggccgacgt gccgcgcgtg
     1441 ctgtggtggt cgaattacag cgccaaggcg gtggcgcaga ttcaggcccg cggcatgccg
     1501 atcgacgtcg cgcggtggaa cctggtgcga gagaacaagc aagccgtcat cgacgagctg
     1561 ctgcgccggt tcgacccgag ccatggcagc gaggagccga tctacacgcc cgagggcgag
     1621 tggagctacg ctcgcttcga gcggtttctc atccgctctg gcgtgacggc gtggccgcgg
     1681 ttggagagcg gcaaactgga catcgatggc gatgcgttcc gaatgatgag ccacatcccc
     1741 ggcatcgagg ccctgcacgc gctgcgcgac acgctcggcg tcatcgtgcg cgcgaagctc
     1801 cctatcggcc atgacgggcg caaccggcca agcctgttcc cgttctgcac cgccaccggt
     1861 cgcaatgcgc acgccaagag cctgttcaat gcgcacgcga gcgtccgctc gttcatggcg
     1921 ttctcgcccg acgcgatcgg cgtctacctc gattggagga cacaggaggt tggaatcgcc
     1981 gcggctctct ccgaagaccg tgcattgatc gaagcgtacc acgatggtga cgtctaccac
     2041 tcgctcgctc tggtttgcgg cctcactgac gacacagacg tgaagcgctg gaaacgggag
     2101 tgcccggacg ttcggcagcg catgaaggcg ctgcagctcg ggatcaatta tgggatgggc
     2161 gtcccttcct tggcgcgcgg cctcgaccgg catcctatca tcgcaagcaa cttcatcgaa
     2221 cgacatcggc gaacgtatcc gcgattctgg gactggcgtg acaacgaagt gcaagtggcg
     2281 atgctggagc gccgaatcga g
//
LOCUS       DATLJT010001714         6298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1799837,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001714 DATLJT010000000
VERSION     DATLJT010001714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799837"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            728..1619
                     /locus_tag="VM910_39020"
                     /pseudo
     CDS             728..1619
                     /locus_tag="VM910_39020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023326.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1843..4077
                     /locus_tag="VM910_39025"
     CDS             1843..4077
                     /locus_tag="VM910_39025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68524.1"
                     /translation="MGWFFRFKLQIRRFSRRHPWLIASVRSFMIFSAAFGGAYGFIAG
                     SRAERSGYDAHAFAIGASFLFAIACVALATLSIRLRFLHRKLRKIALHNEALVDRNWE
                     LQEAEGRARSLFESQSDLIVIRSADSVITFANDAYCQMAGEPREALIGSRHTFGVIEQ
                     GDGALEQSGTRVHDQKIDSPLGPRWIAWREGLVRSDAGQPAELQSVGRDVTDRTETER
                     ALAEARDHADAANRAKSRFLAMASHEIRTPLNGIVGMSGLLLDTSLTPEQTTYVRAIK
                     SSGDALSSLIEELLDYSKIEAGKIDLEQRPFALVSLIEDITELLAPRAEAKKLEIAAY
                     VDERLPHKVIGDAARLRQVLLNLAGNAIKFTQSGGVALIVEPGIWPSEISFLVRDTGI
                     GIAPEARERIFREFEQADERIARNYGGTGLGLSISDRIVKRMGGRITLESHPGAGSTF
                     EVAIPLPASDCDEPSTFAAPDLTARSVMLVAPQNIETSLIARRLQRWGAQTCQISDIT
                     VAQALLPERSWHAVLIDRALGADAVEALADAARVHAMQRIVMFTPATRQEPTSSSALT
                     GYLVKPLRAISLAARLSVAPEAAAPNLASDAAADTENQNEKPTRGLAVLVAEDNEINA
                     LLIRSLLNRLGHQAVIATNGEAALESWLAARSAGAPYDLVLMDIQMPELDGIETAKRI
                     RASEGSEPGPRTPILALTANTLIEDRYACFEAGMDGFLIKPLDRDNLAEALAGLAGTR
                     HMAA"
     gene            complement(4090..5046)
                     /gene="coaA"
                     /locus_tag="VM910_39030"
     CDS             complement(4090..5046)
                     /gene="coaA"
                     /locus_tag="VM910_39030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HVI68525.1"
                     /translation="MDIRATDQQYNPYRIFSREQWARLRDDTPMTLEPGEFSRLKSMH
                     DRLDLQEVEDIYLPLSRLLSIYIDATQRLYYAQRQFLGIRDRKMPYIVGVAGSVAVGK
                     STTARVLQALLARWSPRPKVDLITTDGFLLPNAALERQGLLQKKGFPESYDLPTLLAF
                     LSDIKAGRHPVRAPVYSHLTYDIVPNQWVEIDQPDILIVEGVNVLQTGRLPRDGKAVP
                     YVSDFFDFSVYIDADEPVLREWYVRRFLALRDTAFADPRSYFHRYALLSDDEATATAI
                     AIWERTNLANLEDNILPTRPRATLILKKGADHVVQTVALRRL"
     gene            complement(5061..5384)
                     /locus_tag="VM910_39035"
     CDS             complement(5061..5384)
                     /locus_tag="VM910_39035"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HVI68526.1"
                     /translation="MSRFTVHDLAAIIDARAASGGEASYTRKLLDKGAEHCAKKLGEE
                     AVETVIAAVENDRNHLIAESGDLLYHLLVLLKARGVSFEEVEAALAQRTSMSGLEEKT
                     SRKQT"
     gene            complement(5464..6240)
                     /gene="hisF"
                     /locus_tag="VM910_39040"
     CDS             complement(5464..6240)
                     /gene="hisF"
                     /locus_tag="VM910_39040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVI68527.1"
                     /translation="MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGA
                     DELCFLDITATHENRGIMLDVVRRTAEACFMPLTVGGGVRTIDDINTLLRSGADKVSI
                     NSAAVARREFVKEAAEKFGEQCIVVAIDAKRVKRSAGSDRWEIFTHGGRNATGIDALE
                     YAQEVVSLGAGEILLTSMDRDGTRQGFDIPLTRAIADSIPVPVIASGGVGNLDHLVDG
                     IREGHATAVLAASIFHFGEFTIREAKDHMVRAGLPMRLDP"
BASE COUNT         1178 a         2059 c         1935 g         1126 t
ORIGIN      
        1 gacgtacgat caggtgcgca aggtcttgcc gatgaccttt gtggaccttg gtgctcagca
       61 ggtgaagaac attcaggagc caatcagggc ctatcagggc gttggagttg aaccccaatc
      121 tcgcgactgc ttggatcaac agcggctgga ttaacatctg gcttggacag cctcagcttg
      181 gattggacca cctgatccgt gcccgacgcc tcgatccaag ccccagcagc gtgaggagta
      241 tggcgacggc acatgcatat ttttttctga accgttatga ggaggcgcta tctcaggcgg
      301 agcagcattt atgggcaaat cccgacgcac atcccgcact ccgcattgga gctgcaagcg
      361 ccgctttcgc cgggcgaagg gatacggcac agcgtctagc aggtcacttg caattcgttg
      421 acccagcgtt ccgcgtttcc cgtcttaagg aagttttgga tccctatcag cagtctgcct
      481 tcgtagataa gtactcagaa ggtctgcgtc tggcgggggt gcccgaatga acccgagtgc
      541 accgcctcgt tcccctgtgg cgaacacgcg gccttgtgaa cctcggcggc ggctgcggcc
      601 tttactcgtg aaattttgaa gtcggccccg gcaggcccgc gagaaccgta ggtcgacgtc
      661 aggaaggacc caaatacgca tgagtgcgtt cgaccaaaga cgaaacccaa cctcatacgc
      721 attgccaatc ggctcgcccg gcgccggcaa atcgatgctg gcggcgcgcc tgccatcgat
      781 cctgccgccg ctgtcccctg ccgaattgct cgaagtttcg atgatcgcct ccgtcgccgg
      841 ccaaatcgaa ggcggcgcgc tgacgtcaca acgacccttt cgcgcgccgc atcattccgc
      901 cagcatggcg gctttgaccg gcggcggcat gcgtgcaaag cccggtgaga tttcactggc
      961 gcaccagggc gtgctgtttc tcgacgaatt gccggagttc gacccgcgtg tcttggactc
     1021 gctgcgacag ccgctggaga acggcgaagt ttccgtctcc cgggccaatc atcgggtcac
     1081 ctatccggcg cgtttcatgc tcgttgccgc gatgaaccct tgccgctgcg gccacgcctt
     1141 cgagcccggc tattcctgca agcgcgcccc tatcgaccgc tgcaccgccg actaccagat
     1201 gcgcatctcc ggcccgctga tggaccgaat tgatctgcgg atcgaagtcc cggccgtaag
     1261 cgcagcagac ctgatcctcc cgccgcccgc tgagggctca gcggaggtcg ccgcgcgcgt
     1321 agcggcagcg cgcgaccttc aagccgcgcg ctacgccgca gcaggactgc cgcatatccg
     1381 cacaaacgcg gaagcgcccg cttcgctcct ggaggagatc gcgcaaccgg acgcaaggtc
     1441 aaaagctgtt gcgcgacgcg gccgagacca tgcgcctctc ggcgcgcggc tatcatcgcg
     1501 tactgcgcgt cgcgcgcacg ctcgccgacc tcgacggcgc ggacaaggtc ggccgtctgc
     1561 acctcgccga agccctctcc tatcgcgcgc tcgccgaaga tgtccgccac gcggcctaaa
     1621 accgcgatcc cgcaccaggc cccgcgtatc cgaagcgcgc taaccgcaac gtcgcaaaag
     1681 cgggcgagct tcggtttggg gaaaatgaac cgttaaccct tcatcaaccc tgttctcatc
     1741 gcgtcccgtg gcgtcactcc gcttcaagca ctttgcaaga tagccgacgc atcgtcggcg
     1801 aaagctgcga cagggacgac gacaagccat taaggggcgg cgatgggatg gttcttccgg
     1861 ttcaaactgc aaatccgccg cttctcccga cgccatccct ggctgattgc ctcggtccgt
     1921 tccttcatga tcttctcggc agcctttggc ggcgcctatg gttttatcgc gggcagccgg
     1981 gccgagaggt ccggctatga cgcgcatgcc ttcgcgatcg gagcgagttt cctgttcgcg
     2041 atcgcctgtg ttgcgctcgc gaccctgagc atccggctgc gcttcctgca ccgcaagctg
     2101 cgcaagatcg cgctccacaa tgaagcgctc gtcgaccgca actgggagct gcaggaagcc
     2161 gaaggacggg cgcgcagcct gttcgaatcg caaagcgacc tgatcgtgat tcggagcgcg
     2221 gacagcgtga tcaccttcgc caacgacgcc tattgccaga tggccggaga gccgcgtgaa
     2281 gccctgatcg gcagccgaca cacatttggg gtgatcgagc agggcgacgg cgcgctggag
     2341 cagagcggca cgcgggtcca cgaccagaag atcgacagcc cgttgggacc gcgctggatc
     2401 gcctggcgcg aaggtctggt tcgctcggac gccggtcagc cggcggagct gcaaagcgtc
     2461 gggcgcgacg tgaccgatcg caccgagacc gagcgcgcgc tggccgaagc gcgcgaccat
     2521 gcggatgcgg ccaaccgcgc caagtcacgc ttccttgcca tggcgagcca cgagatccgc
     2581 acgcctctga acggcatcgt cggcatgagc gggctcctgc tcgatacgtc gctgacgccc
     2641 gaacagacaa cctatgtcag ggcgatcaag agctctggag acgcgctgtc gtcgctgatc
     2701 gaggagcttc tggattattc caagatcgaa gccggcaaga tcgacctcga acagcggccg
     2761 ttcgcgctgg tctcgttgat cgaggacatc accgaattgc tggcgccgcg cgcagaggcc
     2821 aagaagctgg agatcgcggc ctatgtcgac gaacgcctgc cgcacaaggt gattggcgac
     2881 gcggcacggc tgcgccaggt gctgctcaat ctcgccggca acgccatcaa gttcacacaa
     2941 agcggcggcg tggcgctgat cgtcgaaccc ggcatctggc ccagcgagat cagctttctc
     3001 gtgcgcgaca ccggcatcgg cattgcaccg gaagcgcgtg aacggatctt ccgcgagttc
     3061 gaacaggcgg acgagcgcat cgccaggaat tatggcggca cgggtctggg cctgagcatc
     3121 tcggaccgaa tcgtcaaacg gatgggcggc cgcatcacgc tggaaagcca ccccggcgcc
     3181 ggctcgacct tcgaagtggc gattcccctg cccgcttccg actgcgatga gccgagtacg
     3241 ttcgccgcgc ccgatttgac cgcccggtcc gtcatgctgg tcgctccgca gaatatcgag
     3301 acgtcgctga tcgcccgccg cctgcagcgc tggggcgccc agacctgcca gatctcggat
     3361 ataaccgtcg cacaagcgct gctgccggaa cgttcctggc atgccgtgct gatcgatcgc
     3421 gcgctcggcg ccgacgcggt cgaagcactc gccgacgccg cgcgcgtcca cgcaatgcag
     3481 cggatcgtga tgttcacgcc tgccacgaga caggagccga catcctcctc cgccctcacc
     3541 ggctatctgg tgaagccgct gcgcgccatc tcgcttgcgg cgcggctttc ggtggcgccc
     3601 gaggcggccg cacccaatct tgcaagcgac gctgcggccg acacggagaa ccagaacgag
     3661 aagccgacgc gcggccttgc cgttctggtc gccgaagaca acgagatcaa tgccttgctg
     3721 atccgctcat tgttgaaccg gctcggacat caggccgtga tcgcgaccaa cggcgaggcg
     3781 gcgctggaat cctggcttgc cgccagatcc gcaggcgccc cttacgacct cgtcctcatg
     3841 gacatccaga tgcctgagct cgacggcatc gagacggcca agcgcatccg tgccagcgaa
     3901 ggcagcgagc cgggcccgcg cacgccgatc cttgcgctga ctgcgaatac gctgatcgag
     3961 gatcgctatg cctgtttcga agccggcatg gacgggtttt tgatcaagcc gctcgatcgc
     4021 gacaatctcg ccgaagcgct ggcgggcctt gccggcacgc ggcacatggc agcgtagctt
     4081 cagcgcactt cagagccgcc gcagcgccac ggtttgcacc acgtgatcgg cgcccttctt
     4141 caggatcaac gtcgcacgcg ggcgcgtcgg cagaatgttg tcctcgagat tggctaggtt
     4201 ggtacgttcc cagatcgcga tcgcggttgc ggtggcttcg tcgtcggaca gcagcgcata
     4261 gcgatggaaa taggatcgcg gatcggcgaa cgccgtgtcg cgcaaggcga gaaagcgcct
     4321 gacataccac tcacgcagca ccggctcgtc ggcgtcgata taaacggaga aatcgaaaaa
     4381 gtcggacacg taaggcaccg ccttgccgtc gcgcggcaga cggccggtct gcaggacatt
     4441 gacgccttcc acaatgagga tgtcgggctg atcgatctcg acccactggt tcggcacgat
     4501 gtcgtaggtc aagtgcgaat aaacgggtgc gcgcaccgga tgccgtcccg ccttgatgtc
     4561 gctcaggaac gccagcagcg tcggcaaatc gtagctctcc ggaaagcctt ttttctgcag
     4621 caggccttgc cgttccagcg ccgcattggg aagcaggaag ccatccgtcg tgatcaggtc
     4681 gaccttcggc cgcggcgacc agcgtgccaa cagcgcctgc agcacgcgcg ccgtggtgga
     4741 tttgccgacg gcgaccgaac cggcgacacc aacgatgtaa ggcatcttgc ggtcgcggat
     4801 gccgaggaat tggcgctggg cgtaatacag ccgctgggtg gcgtcgatat agatcgagag
     4861 cagccgcgac agcggcaggt agatgtcctc gacctcctgc aggtcgaggc gatcatgcat
     4921 cgatttcaaa cgggagaact cgcccggctc cagggtcatg ggcgtgtcgt cgcgcaagcg
     4981 cgcccactgt tcgcgagaga agatgcggta aggattatat tgctggtctg ttgcgcggat
     5041 atccatcagg ccgcctctgg ctatgtctgc ttgcgcgacg tcttctcttc gagtccggac
     5101 atcgaagttc gttgcgccaa ggcggcttca acttcttcga aactgacgcc tcgcgccttc
     5161 aggagcacca ggaggtgata gagaaggtcg ccgctttcgg cgatcagatg attgcggtcg
     5221 ttctcgaccg cggcgatcac cgtttccacc gcctcctcgc ccagcttctt ggcgcaatgc
     5281 tcggcgccct tgtccagcag ctttctcgtg taagacgcct cgcccccaga tgccgcgcgg
     5341 gcatcgatga tggcagccag gtcgtggacg gtaaaacgcg acatcaacaa aactcggcac
     5401 acatgtccgc agaaaatgcg ggcgcttcag ccgctcatgt agcactttta tgacgaggag
     5461 tcgtcaggga tcgagccgca tcggcaaccc ggcgcgcacc atgtggtcct tcgcttcgcg
     5521 aatggtaaat tcgccaaagt gaaagatcga ggccgccagg accgcggtcg cgtggccctc
     5581 gcggatcccg tccaccaggt gatcgaggtt gccgacgccg cccgaggcga tcaccggcac
     5641 cgggatgctg tcggcaatcg ctcgggtcag cgggatgtca aaaccctgtc tcgtgccgtc
     5701 ccgatccatc gaggtcagca gaatttcccc cgcccccaac gacaccacct cctgggcgta
     5761 ttcgagggcg tctatcccgg tggcattacg tccaccatgg gtgaaaatct cccagcgatc
     5821 cgagccggcg ctccgtttca cgcgcttggc atcgatcgcc accacaatgc actgctcgcc
     5881 gaatttctcc gcagcttcct tgacgaactc acgcctcgcg accgcggccg agttgatcga
     5941 caccttgtcg gcgccagagc gcaacaacgt gttgatgtcg tcgatggttc gtacgccgcc
     6001 gccaacggtg agcggcatga agcaggcttc ggccgtgcgc cggaccacgt ccagcatgat
     6061 gccgcggttt tcatgggttg ccgtgatgtc gaggaaacag agttcgtcgg cgccggctgc
     6121 gtcataggca atcgccgctt ccacgggatc gccggcatcg cgcaaatcga cgaaattgac
     6181 gcccttcacc acgcggccgt ccttgacgtc cagacaggga atgacgcgca ccttgaacat
     6241 ctgaaatctc ctaggcggcg gcgcgcgcgt ttttgatgag ctcgagcgct gccgatgc
//
LOCUS       DATLJT010001715         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2946150,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001715 DATLJT010000000
VERSION     DATLJT010001715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2946150"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VM910_39045"
     CDS             complement(<1..617)
                     /locus_tag="VM910_39045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68528.1"
                     /translation="MGTADSSRRIRVGDIELAYRITGRGKPVILIHGLACGQRMWFHQ
                     RRKLSDRHTVVTYDQRGHGHSDAPDDATRYSGAQLARDLAGIVDALSFDKVAIVGFSM
                     GGGPALALAAARPERITHLVLADIGSGADDAWRIQWLARRWVDFAERTGWDELLPDML
                     RSEFFKSYANRGPRYRRHMGGLIRSTPLAGLRHTLSQVLGQRKSL"
     gene            796..1419
                     /locus_tag="VM910_39050"
     CDS             796..1419
                     /locus_tag="VM910_39050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68529.1"
                     /translation="MRCAMPLSMLLSLSILGLVSVTFANDASAEVACSGHKLLPIPRP
                     QSSCSDVKPRIYPSPDGALRALVFPVDVSLYATPDMESRVVIRTSKGDTLTSKDYSSP
                     RGANGYYVFNAKWSPDSQFFVYSMSSSGGHSPWSFPMMVYSRQKNRIAGFSDMTNGEP
                     TVSGDFHFAGAHTVVASTWKQPASLDDKVAVTVDLEEAFAKLAPSAD"
     gene            complement(1539..2915)
                     /locus_tag="VM910_39055"
     CDS             complement(1539..2915)
                     /locus_tag="VM910_39055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI68530.1"
                     /translation="MRQMTLVAFLQAQNCTNLPGSWRHPHSRTDTWSADYYQHIGRVL
                     EEGKFHLGFFDDRLSMPDMYGRDHEHTVRHGIRCVKMDAVTVLTVMGMATERLGLGAT
                     YSTSYYEPFHVARLFATLDLMTDGRAAWNVVTSLNDGEAQNMGREQVIEHDLRYDRAD
                     EFMEVVFGHWDSWEDDAIVQDKATGLFANPEKVHRLDHNGRFFRSRGPFTVPRSSQGR
                     PVIIQAGQSGRGKRFGAQWGELIFVVYPNIAVAKRDYAEFKAEVAACGRDPEHVKITQ
                     LINTVAAATKVEAEDRWALISKLPLEIDALSLLSEALNFDFSTKKMDEAFTDEEMAAI
                     SGLQAIRDRVVRASGNKNPTVRDFIHFSGRGRVHDAIVGGPKEICDELEQWLVERACD
                     GFVVSATHVPGAYEDFVRFVVPELQRRGLFRTAYAGKTLRENLGMPIPPVAAWRGMRA
                     RRQRDGVG"
     gene            complement(3144..>3885)
                     /locus_tag="VM910_39060"
     CDS             complement(3144..>3885)
                     /locus_tag="VM910_39060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HVI68531.1"
                     /translation="LLTAAVVLLLLLIVTRIVQRWLETELLPRTRLEPSLQLSIVTIF
                     GYIGAIIAIVVALTGLGFDLQKITLIAGALSVGIGFGLQSIVSNFVSGLILLTERPIR
                     VGDSIVVKGEEGWVRRVRVRATEIETFDRASVIIPNSEFVTGVVKNWTRTNTLGRIVI
                     KVVVAYDSDPVQVREILTDVAGNHPQIVQSPPPGAFLLGFGVSLAHSALEFELRCVVA
                     DVEKALSVRSDLHYAIIKRFREAGIAIP"
BASE COUNT          746 a         1212 c         1217 g          710 t
ORIGIN      
        1 aaaagcgatt tccgctggcc cagcacttgc gacagggtat gtcgcaatcc ggcgagcggc
       61 gtgctgcgga tcaagccgcc catgtggcgg cggtaacgcg gcccgcgatt ggcatacgat
      121 ttgaaaaatt cgcttcgcag catgtccggg agcaactcat cccagccggt gcgctccgca
      181 aagtcgaccc agcgccgcgc cagccactgg attcgccagg cgtcgtccgc acccgatccg
      241 atgtcggcga ggacgagatg cgtgatccgc tcgggccttg cggcggcgag cgcaagagcc
      301 gggccgcctc ccatggaaaa gccgacaatc gcaaccttgt cgaagctgag cgcatcgacg
      361 ataccggcga ggtcgcgggc gagctgagcg cccgagtaac gggtggcgtc gtccggggca
      421 tcgctatggc cgtggccgcg ctgatcgtag gtgacgaccg tatggcgatc ggacaatttc
      481 cgccgctggt gaaaccacat ccgctggccg cacgcgagcc cgtgtatgag gatcacgggc
      541 ttaccgcggc ctgtgatgcg ataggcgagc tcgatatcgc cgacgcgaat gcggcgcgag
      601 gagtcggccg tgcccatcga cgcaggtgat cctcgtatcg ccctcgagca caagacggca
      661 tggtggaaga agccgaccgc cggcagctcg ggcccgcaac gacatgacag agtttaaata
      721 ggacatccga gcagcgaaag ggcatgcttc cgcgcttgct cgtccgtagc gtctcttggt
      781 cctggaggaa cgatcatgcg ttgcgcaatg ccattatcga tgttgctttc gctctcaatc
      841 ttgggcctgg tgtcagtgac gtttgccaac gacgcgtccg ccgaggttgc ctgcagcgga
      901 cacaaattgc tgccgatccc acgaccgcag tcgagctgca gcgatgtcaa gccgcggatc
      961 tacccatcgc ccgacggcgc gctccgcgcg ctcgtgtttc ccgtggatgt cagcctctat
     1021 gccacgcccg acatggagag ccgcgtggtc atccgcacca gcaagggcga tactctcacg
     1081 tcgaaggact attcctcgcc gcgcggcgcc aatgggtact atgtcttcaa tgcaaaatgg
     1141 tcgcccgact cgcaattctt cgtctacagc atgtcgtcgt ccggcggtca ttcgccctgg
     1201 tcatttccta tgatggtgta tagccgccag aagaaccgca tcgccggttt cagcgacatg
     1261 accaacggcg agccgacggt ttcgggagat ttccattttg ccggcgcgca tacggtggtc
     1321 gccagcacgt ggaagcaacc tgcatcgttg gatgacaagg ttgcggtcac ggtcgatctc
     1381 gaggaggcat tcgcaaagct cgccccgtct gcagattaga ggccggcagg gcggacagcc
     1441 cgggtgaccc atgtgaagcg ctgctcgcgg cgagaaggcg aaatcgccat ccgcgccagg
     1501 ggccagattc gccgggttcg tgcgggggat cagggtgctc aaccaacgcc atctcgctgc
     1561 cgtcgcgcgc gcatgccgcg ccatgcagct accggcggga tcggcatccc tagattctcg
     1621 cgcagggttt ttcctgcgta cgccgtacgg aacaagccac ggcgctggag ctcgggcacg
     1681 acgaagcgaa cgaagtcctc gtaggctccg ggaacgtggg tcgcggacac gacgaagccg
     1741 tcgcaagcgc gctcgaccag ccattgttcg agttcatcac aaatctcttt cggcccgccg
     1801 acgatcgcat catgcactcg gccgcggccg ctgaagtgga tgaagtcgcg caccgtcgga
     1861 tttttgttgc cgctcgcgcg cacgacgcgg tcgcggatcg cctggaggcc ggagattgcc
     1921 gccatttcct cgtccgtgaa cgcttcgtcc atcttcttgg tcgagaagtc gaaattgagc
     1981 gcttccgaca gcagcgagag cgcgtcgatc tcgagcggca gcttgctgat gagcgcccat
     2041 cgatcctcgg cctcgacctt ggtggcggcg gccaccgtgt tgatcagttg cgtgatcttg
     2101 acgtgctcgg ggtcgcggcc gcaggcggcc acctccgcct tgaattcggc atagtcgcgc
     2161 ttggcgacgg cgatgttggg gtaaacgacg aagatcaact cgccccattg cgccccgaag
     2221 cgcttgccgc ggccgctttg gcccgcctgg atgatgacgg ggcggccctg cgatgagcgc
     2281 ggcacggtga acggcccgcg ggagcggaaa aaacggccat tgtggtcgag gcgatggacc
     2341 ttttccgggt tggcgaacag gcccgtcgcc ttgtcctgca cgatagcgtc atcctcccag
     2401 ctatcccaat ggccgaacac cacttccatg aattcgtcgg cccgatcgta gcgcaagtca
     2461 tgctcgatca cctgctcgcg gcccatgttt tgcgcttcgc cgtcgttgag cgaggtgacg
     2521 acgttccagg ctgcacgtcc atcggtcatc agatcgagcg tggcgaacag gcgggccacg
     2581 tggaacggtt cgtagtagct ggtcgaatag gtcgcaccga ggccgagccg ctcggtcgcc
     2641 atgcccatga ccgtaagcac ggtgaccgca tccatcttca cgcaacggat gccgtggcgc
     2701 accgtgtgtt catggtcgcg cccgtacatg tcgggcatcg ataaccggtc gtcgaagaag
     2761 ccgagatgga acttcccctc ctccagcacc cggccgatat gctgatagta gtccgcggac
     2821 caggtatccg tacgcgaatg cggatggcgc cacgaacccg ggagattcgt gcaattctgc
     2881 gcttgcagga aggcgaccaa ggtcatctgg cgcattgtcg ggacccttcg caatctcgag
     2941 cgcgctgcgg atcgtgccag tacgtatgcg cttgacggcg ctcgatcgcg cgaccccttg
     3001 gcgagcttga ggaagctcac ggccaaaagg agggctcgta tccccaacct atgggatcga
     3061 acgtatcgtt ggaaagccca gcctgtcgag agtcagaacg cttcgcttgc ctccgcaagt
     3121 tggcggttcg ggacgccgtg gtgttacgga atcgcgatgc ctgcctcgcg aaaccgcttg
     3181 atgatggcgt aatgcaggtc gctgcggacc gacagggcct tttccacatc ggcgacgacg
     3241 caacgcagtt cgaattccag cgcgctgtgt gcgaggctca ccccgaagcc caagaggaat
     3301 gccccgggag gcggagactg cacaatttgg ggatgattgc cggcgacgtc agtcaagatt
     3361 tcacggacct gaaccggatc gctgtcatag gcgacgacga ccttgatcac gatgcggccc
     3421 aatgtattcg tgcgcgtcca gttcttgacg acgccggtga cgaattcaga gttcgggatg
     3481 atcacgctcg ctcgatcaaa agtctcgatc tcggttgcgc gtacacgaac acgccgcacc
     3541 catccctcct cgcccttgac cacgatcgag tcgccgacgc ggatcggccg ttcggtcagc
     3601 aggatgaggc cggatacgaa attcgacacg atggactgca gcccaaaccc gataccgacc
     3661 gaaagggcac cggcaatcaa cgtaatcttc tgcaggtcga agccaagccc cgtgagcgca
     3721 acaacgatcg ctatgatcgc gcctatatag ccgaagatcg tgacgatcga gagctggagg
     3781 ctgggctcca atctggtccg cggcagcagc tccgtctcca gccaacgttg cacgatgcgc
     3841 gtgacgatga gtaacagcag aaggacaaca gcggccgtca gcagc
//
LOCUS       DATLJT010001716         6297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3637976,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001716 DATLJT010000000
VERSION     DATLJT010001716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637976"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1232)
                     /locus_tag="VM910_39065"
     CDS             complement(246..1232)
                     /locus_tag="VM910_39065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68532.1"
                     /translation="MLRRRELLGLVLGATAARPPRVFAQQVASARLGFIHSSAPGYFE
                     PFRAAFLAGLKEAGYTEHSNLAIESRWAEGHYERLPALVTDLLDQRVDVLFAAGGTDP
                     ARAAKAATQTTPIVFVSAADPVASGLVASLSRPGGNVTGVSLLASALSGKKFALLREL
                     VPRAAAIGALVNPGYAGVKSQVDEFQAAAAQLGVKLVVLSAETDEDIKTAFASLTQQH
                     IGALVIANDLFFGGRRDQLVELAATYGVPVMHFQREFVTAGGLMSYGPSFDDGYRQGG
                     VYVGRILRGEKPADLPVMQPTKFELVINLKAARALGITVPPSLMAIADEVIE"
     gene            complement(1386..2138)
                     /locus_tag="VM910_39070"
     CDS             complement(1386..2138)
                     /locus_tag="VM910_39070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68533.1"
                     /translation="MKSGAESLTPFVVRGARLFDRIDYRLIETPEERDSVYLMRYRAY
                     LHGGLISPSESQRIIDCYDDAPNAWLFGIYLDGELCSSLRVHVLTSEWRMSYSTELFG
                     DVLHSRLDRGEVFIDPARFAADPEKSQRFQELPYLTVRLGYLACDHFNADTGLAMVRT
                     DHQAFYRRVFLSETITEPRAFPGWHSRKVALMASDFPTVREKILARFPILRSSAFERR
                     MLFDRGSQRKAAPRPWLVSVPRRHDAAAVSLG"
     gene            complement(2283..4613)
                     /locus_tag="VM910_39075"
     CDS             complement(2283..4613)
                     /locus_tag="VM910_39075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI68534.1"
                     /translation="MQLASQRGEQNQEVSPRISAALIDSLFEASGTVLTGVVFSAIAA
                     GMTALKTGQGLLWAFVLLLMAAGAFRAFDLRRYQASKSTLTAEEAAQWQKRYQLGAMI
                     QAAAIGLWCATTLLSNDDAVAHMICLSVTTGIVAGAAGRAYGRQSIFRLQAVLMFGPT
                     VIALALRGTGYYVAMSVVCAAFLAAVIQISANLHRIFVRAVVAREREAALAGQFDTAL
                     NNMPHGLCMFRADGRLAVMNHRFSEMMGLADDIAQHEIRAPGVVEACVQAGSISVASG
                     KIIVAEIENSQARDIVTTDPDAARGRSLSWTFQPMADGGAVVLLEDITERRNAEARIS
                     HLARFDELTGLPNRVNFRDEIEHLLTMPHEEGHLSALLFIDLDQFKQVNDTLGHPCGD
                     QLLCAVAARLRDMLRPEDFVARFGGDEFVVFQQNIRSAEDAAMLARRIVDHLSERYKI
                     DDHLVEIGASIGIAMTSFGVSADTLLKNADMALYRAKADGRGTFCFFRDELAQAVESR
                     RILELDLRKALANEEFELYFQPLVNLKSGKITTCEALLRWNHPVRGTVSPVDIIPVAE
                     DMGLIVDLGRWILRKACLECMKWPDGVSVAVNFSPQQFHQRDLLSEVRYALEVSTLPA
                     NRLEIEITESSLLRNTQLTHDVLSQLRAIGVRISLDDFGTGYSSLSYLHNFPLQKVKI
                     DRSFLEGIDTDRPLTLLRGVARLSADLGMSVVVEGIETNEQLELISADGTVTEAQGYL
                     FSRPVPAIRVRQLLNASHGRHMVDDQVVAVPSRSIA"
     gene            complement(4786..6138)
                     /gene="tolB"
                     /locus_tag="VM910_39080"
     CDS             complement(4786..6138)
                     /gene="tolB"
                     /locus_tag="VM910_39080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HVI68535.1"
                     /translation="MPMTQFPLPPLMLDRRKLLLGGAGAAAGLLLPSFASAQTKFTVP
                     EGTVAPLPIAIPNFVPGTPGDTEVGAGVAQVITNNLKRSGLFAPIDPAAFIDRITDSD
                     KQPQFQNWKTINAQALVVGRTTRQNDGRLKAEFRLWDVASGQQLAGQSFVTAPEYWRR
                     IAHIISDQVYERLTGDKGYFDSRVVFVDETGPSERRVKRLALMDQDGANVRYLTRGAD
                     LVLTPRFSPSTQEITYMEFGQGDPKVYLFNIETGQREIVGNFPGMSFSPRFSPDGQHI
                     IMSLQQGGNSNLFVMDLRSKSMTRLTDTPAIDTSPSYAPDGSRICFESDRGGKPQIYV
                     MPAQGGQAQRISFGEGSYSTPVWSPRGDYIAFTKQGGGQFAIGIMKTDGSGERILTSG
                     FHNEGPTFAPNGRVLMFFRDPGGNSGPSLFTVDISGRNEQRVPTPGFASDPAWSPLLS
                     "
BASE COUNT         1277 a         1929 c         1974 g         1117 t
ORIGIN      
        1 aaacgaacgt tccgtcaacc ttgatggggt gttgcggaac ccgatcaaat gccgagaaac
       61 ggcgaaaagc gaccgcgata gttaatcggt tgtaaatgtg gcgcgagggt gaaggcaagc
      121 tccgggggta tcggaaatgc ccggattttg ggccggagtt gctcttattc cacaccttcc
      181 acgcgcgcga cgagcccgga cgacggcagg cgttttttgc tcgcgagcgt taaaatcagc
      241 ccgatttact cgatgacctc gtcggcaatc gccatcaacg aaggtggaac cgtgataccg
      301 agcgcgcgtg ccgccttgag attgatgacg agctcaaact tcgtcggttg catgaccggc
      361 agatcggccg gcttttcgcc cctgagaatg cggccgacat agacgccgcc ctggcggtaa
      421 ccgtcgtcga agctcggtcc gtagctcatc agaccaccgg cggtgacgaa ctcccgctgg
      481 aaatgcatga cgggcacgcc gtatgtcgct gcgagctcga cgagctgatc ccgacggccg
      541 ccaaagaaca gatcattggc gatgacgagc gcgccgatgt gttgctgggt caagctcgcg
      601 aaggcggtct tgatgtcttc gtccgtttcg gcggagagca cgaccagttt gacaccgagc
      661 tgggccgccg cggcctgaaa ttcatccacc tgcgatttca cgccggcata gcccggattg
      721 acgagcgcgc cgatcgcggc cgcacgaggt accagttcgc gcagtagggc aaacttcttg
      781 ccgctcagcg ccgaagcgag caggctcacg ccggtgacgt tgccgcccgg ccggctcagg
      841 ctcgcaacga ggcctgacgc gacgggatct gccgcgctca cgaacacgat cggcgtcgtc
      901 tgcgttgctg ccttcgccgc gcgcgccgga tcggtgccgc cggcggcaaa gaggacatcc
      961 actctttgat caagcaggtc ggtcaccagc gccggcagcc gctcatagtg gccttccgcc
     1021 cagcgcgatt cgatcgcgag attgctgtgc tcggtgtaac cggcctcctt gagaccggcc
     1081 aggaacgccg cgcgaaacgg ctcgaagtaa ccaggagccg aactgtggat gaagccgagg
     1141 cgggcagacg ccacttgctg cgcgaatacg cgcggcggac gcgccgcagt ggcacccaga
     1201 acaagcccga gaagttcccg ccgccgcagc atgccctttc cccgatcggt gccaccacga
     1261 tagcatagcg ccggattcag gtaacgcagc cctctctgcc gccgatagca ttctgtcggg
     1321 tttgcggaaa cgacggcggc gcgcttgaag cggcgatcac gctcgaatgc aggacggaac
     1381 ggcggttagc ccaaggatac ggctgcagca tcatgacgac gcggcaccga tacgagccac
     1441 ggccgcggcg cagccttgcg ctggctgccg cgatcgaaca gcatacgccg ctcgaaagcg
     1501 ctcgaacgca aaatgggaaa gcgcgccaag atcttttccc ggacagtcgg gaaatcggac
     1561 gccatcaagg ccaccttcct cgaatgccag cctggaaacg cgcgcggttc ggtgatggtc
     1621 tcggacagaa agacacggcg atagaacgcc tggtgatcgg tacgcaccat cgcgagcccg
     1681 gtatcggcat tgaaatgatc gcatgccaga tagcccagcc gcaccgtcag ataagggagc
     1741 tcctgaaacc gctgactttt ttccgggtct gcagcaaaac gagccggatc gatgaaaact
     1801 tcgcctcgat cgaggcgact atgcagaaca tcgccgaaca attcggttga ataggacatc
     1861 cgccattctg acgtcaggac gtgaacgcgg agagaactac aaagttcgcc gtcgagatat
     1921 attccgaaga gccaggcatt cggcgcatcg tcataacaat cgatgatgcg ctgggactcc
     1981 gacggcgaaa tcaatccgcc atgcagatag gctctgtagc gcatcaaata gaccgaatct
     2041 ctttcctcgg gagtttcgat gaggcggtaa tcgatgcgat caaacagccg tgcccctcgt
     2101 acaacgaacg gagtgagcga ttcggctccg gacttcatct gacgtccccc acactgccaa
     2161 gcaacttccg cgagtgagag aagattaacg gtgccttaac agaattgcaa agactcgaag
     2221 atgttagggt taatgttcct tgcccgcggc tgccaccggg cgagaggact catcgagtaa
     2281 ggtcaggcga tcgaccgcga agggacggcg acgacctgat cgtccaccat gtggcggccg
     2341 tgcgatgcgt tgagcaactg gcgcaccctg atcgctggga ccggtcggct gaacagatag
     2401 ccctgcgctt ccgtcaccgt gccgtcggcc gaaatcagct cgagctgctc gttggtctcg
     2461 atgccttcga ccacgaccga catgccgaga tcggcggaga gccgcgccac gccgcgcagc
     2521 agcgtcagcg gccgatcggt gtcgatgccc tcgaggaagg agcggtcgat cttcaccttt
     2581 tgcagtggga agttgtgcag atagctcaag ctcgaatagc cggtaccgaa atcgtcgagc
     2641 gaaatcctta cgccgatggc gcgcaactgc gacagcacgt cgtgggtcaa ctgggtgttg
     2701 cgcagcagcg aggattcggt gatctcgatc tcgagccggt tcgccggcag tgtggagacc
     2761 tcgagcgcgt agcgaacctc gctcagcaag tcgcgctgat ggaattgctg cggcgagaaa
     2821 ttgaccgcga cgctgacgcc atcgggccat ttcatgcact ccaggcacgc cttgcgcagg
     2881 atccagcggc cgagatccac aatcaggccc atgtcctccg cgaccggaat gatatcgacc
     2941 ggggagaccg tgccgcgcac gggatgattc cagcgcaaca acgcctcaca ggtcgtgatc
     3001 ttgccggact tgagattgac cagcggctgg aagtacagct cgaactcctc gttcgccagc
     3061 gccttgcgca ggtcgagttc caggatgcgg cgcgactcga cggcttgcgc aagctcgtcg
     3121 cggaagaaac aaaacgtgcc gcggccgtcg gccttcgcac ggtaaagcgc catgtcggcg
     3181 ttcttgagca aggtgtcggc gctgacgccg aacgatgtca tcgcaatgcc gatgctggcg
     3241 ccgatctcga ccagatggtc gtcgatcttg taacgctcgc tcaaatgatc gacgatgcgc
     3301 cgtgccagca tggcggcgtc ttcggcggaa cggatatttt gctggaacac cacgaattcg
     3361 tcgccgccga aacgggcaac gaaatcctct ggacgcagca tgtcgcgcaa tcgcgccgcg
     3421 acagcgcaca acagttggtc gccgcagggg tgaccgagcg tatcgttgac ctgcttgaac
     3481 tgatcgaggt cgatgaacaa aagggccgac agatgccctt cttcgtgcgg catggtcaga
     3541 aggtgctcga tttcgtcgcg gaaattgacg cggttgggca ggccggtgag ttcgtcgaac
     3601 cgcgcgagat ggctgatacg ggcttccgcg ttgcgccgct ccgtgatgtc ctcgagcagc
     3661 acgacggcgc cgccatcggc catcggctgg aacgtccagg acagcgagcg gccgcgggcc
     3721 gcgtcgggat cggtcgtgac gatgtcgcgc gcctgcgagt tctcgatttc ggcaacgatg
     3781 atcttgccgc tcgcaaccga gatcgatcct gcctgcacgc aggcctcgac gacgccgggg
     3841 gcacggatct cgtgctgagc gatgtcgtcg gccaggccca tcatctcact gaagcgatga
     3901 ttcatgacgg ccagccggcc atcggcgcgg aacatgcaca gaccgtgcgg catgttgttc
     3961 agcgccgtgt cgaactggcc cgcgagcgcc gcctcgcgtt cacgcgctac gaccgcccgc
     4021 acgaatattc ggtgcaaatt ggccgaaatc tgtatgacgg ccgcaaggaa agcggcgcac
     4081 acgaccgaca tcgccacgta gtagccggta ccgcgcagcg ccagcgcgat cacggttgga
     4141 ccgaacatga gcacagcctg cagacgaaat atcgattgcc gtccgtaggc cctgcctgca
     4201 gcgcccgcca cgatgcccgt cgtgaccgac aggcagatca tgtgggccac ggcgtcgtca
     4261 ttgctcagca gcgtggtagc acaccacagg ccgatcgcag cggcctggat catcgcgccg
     4321 agctgatagc gcttctgcca ctgcgccgct tcttcggcgg tcaaggttga tttgctcgcc
     4381 tggtaacgcc gcaaatcgaa tgcgcgaaaa gctcccgccg ccatgagaag caggacaaat
     4441 gcccagagca gaccttgtcc cgtcttcagc gcagtcatgc ctgctgcgat ggccgagaag
     4501 acgacgcccg taagcaccgt gccggaagcc tcgaacagcg aatcgatcag cgccgccgaa
     4561 attctcggcg aaacctcctg attctgctct ccgcgttggc ttgcgagctg catttgaaga
     4621 cggcgcgtcc tgttcaacgc gcagttctac aatttcgaaa tgaagccttt ctgagcgaac
     4681 atcgttaccg ggacgttgtc ctcgatcgaa aaagccggga atatgcccgt taaatcagcg
     4741 gactaggcag gctttgccgg gtgtgtcaga gcccgggtac aactatcagg ataacagcgg
     4801 cgaccacgcc ggatcagagg cgaaaccggg ggtcggcacg cgctgctcgt tgcgcccgga
     4861 aatgtcgacg gtgaacagcg acggtccgct gtttccgccg ggatcgcgga agaacatcag
     4921 gacgcgaccg ttcggagcaa aagtcgggcc ctcattgtgg aagcccgagg tgaggatgcg
     4981 ctcgcccgag ccgtccgtct tcatgatgcc gatcgcgaac tggccgccgc cctgtttggt
     5041 aaacgcaatg taatcgccgc gtggcgacca caccggcgtt gaatagctgc cctcgccgaa
     5101 cgaaatgcgc tgcgcctggc cgccctgtgc cggcatgacg tagatctgcg gctttccgcc
     5161 gcggtcggat tcgaagcaga tgcggctgcc gtcgggcgcg taggatggcg aagtgtcgat
     5221 cgcgggcgtg tcggtcagtc gcgtcatcga cttggagcgc agatccatca cgaacaggtt
     5281 ggaattgccg ccctgttgca aactcatgat gatgtgctgc ccgtcgggcg agaagcgtgg
     5341 cgagaacgac atgcccggga agttgccgac gatctcgcgc tggccggtct cgatgttgaa
     5401 aagatagacc ttcggatcgc cctgaccgaa ttccatgtag gtgatttcct gcgtcgaggg
     5461 cgaaaagcgt ggcgtcagca cgagatccgc gccgcgcgtc agatagcgca cattggcgcc
     5521 gtcctgatcc atcaacgcca gccgcttgac gcgccgttcc gacggaccgg tctcgtcgac
     5581 gaacaccacg cggctgtcga aataaccctt gtccccggtc aggcgctcat agacctggtc
     5641 ggaaatgatg tgcgcgatgc gccgccagta ttcgggcgcg gtgacaaagg actggccggc
     5701 gagctgctgg ccggaggcga cgtcccacag gcggaattcc gccttcaggc gtccatcgtt
     5761 ctggcgcgtt gtccgtccga ccaccagcgc ctgcgcattg atggtcttcc agttctggaa
     5821 ctgcggctgc ttgtcgctgt cggtgatgcg gtcgatgaag gcggccggat caatcggcgc
     5881 gaaaaggcct gagcgtttga gattgttggt gatcacctgc gcgacgccgg cgccgacttc
     5941 ggtgtcgccg ggcgtgcccg gcacgaaatt cggaatcgcg atcggcagcg gcgcgaccgt
     6001 accttcgggc accgtgaatt tcgtttgggc gctcgcaaac gacgggagca gcagaccggc
     6061 agccgccccc gcgccgccga gcagaagttt gcggcggtca agcatcagcg gaggaagcgg
     6121 aaactgcgtc atgggcatcg cggctctcaa acatcaactt cgaaacatcg ccttggggtc
     6181 gaacgtgatc tccatgtcct tccaagcgtc gtagctctca tcgcgcagca tgttgaaggg
     6241 ctggccttcc agcactgcgc ggcgcgcagc ttcggcggca acctgatatg tcggcga
//
LOCUS       DATLJT010001717         1586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1339959,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001717 DATLJT010000000
VERSION     DATLJT010001717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339959"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VM910_39085"
     CDS             complement(<1..603)
                     /locus_tag="VM910_39085"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HVI68536.1"
                     /translation="MAAFIKRAFEAAGLRSEDAKVVADLMVEADLLGSDTHGVIRLPL
                     YLRRLKAGGINARPDIRIVQERPATALVDGDNGIGHLVMRFAAMTAIEKAKGAGVAWV
                     GARMSNHAGPAALYAMMPLAHDMIGLYLAVGSNNHLPPWGSTENLLVTNPVAIAVPAE
                     EEPPIVLDMAPTVAAFGKVRLKMQRGEEMPVGWMIGRDGKP"
     gene            906..>1586
                     /locus_tag="VM910_39090"
     CDS             906..>1586
                     /locus_tag="VM910_39090"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVI68537.1"
                     /translation="MARYRVTVDTGGTFSDFVYLNEETGELSIAKLPSTPDDPSRAIL
                     AGIEALLATAGEGADIGYFCHGTTVGTNALLEGKGVRTGLLVTEGFRGIYLVGEQARP
                     YGTAIFDVMYDKPAPLVPPSLTGEVKERVDFRGNVLRDLDETALRETVRELTARNVES
                     IAVCLLFSFLHPAHEARVREIIVEEMPDCSISLSSEVLPQIREYYRLSTTVINAYLQP
                     ILARYIAQL"
BASE COUNT          265 a          526 c          501 g          294 t
ORIGIN      
        1 cggcttgccg tcgcggccga tcatccagcc gaccggcatc tcctcgccac gctgcatctt
       61 caggcgcacc ttgccgaagg cggcgaccgt cggcgccatg tcgagcacga tcggcggctc
      121 ttcctcggcc ggaacggcga tcgcgacggg attggtgaca agcaggtttt cggtcgaacc
      181 ccagggcggc agatggttgt tgctgccgac cgcgaggtaa agcccgatca tgtcatgggc
      241 gagcggcatc atggcataga gcgccgccgg gcccgcgtgg ttgctcatgc gtgcgcccac
      301 ccaggcgacg cctgcgcctt tcgccttctc gatcgccgtc atcgcggcaa agcgcatgac
      361 caggtggccg atgccattgt cgccgtcgac aagggcggtg gccggcctct cctgcacgat
      421 ccggatatcc ggacgcgcgt tgatcccgcc cgccttgaga cgtcgcagat aaagcggcag
      481 gcgaatcaca ccgtgggtat cgctgccaag cagatcggcc tcgaccatca aatcggcgac
      541 caccttcgcg tcctcggagc gaaggccggc ggcctcgaag gcgcgcttga tgaaagctgc
      601 caacgccggc gccgcgatgc gcggcgcatc ggcgacgttc gcggtcttgg catcgctggc
      661 gacgcgtcct gacatgttca ttccgatcta gcgcctgccg ctatgtcatc ttgatcgagg
      721 gtcgattaca actggcattt cttcgcccca acgttcgttc cgcgagatcg gcgcgatcca
      781 agttccctcc ccttgcgcta tgccgcggcg cggtcgggac gccatcagcc cgcgatattg
      841 tcctttaagg ccggtcatgt ccttatagcg ggcgctgctc gctcccggct ggaaccgatc
      901 atcgcatggc tcgctaccgc gtcacggtcg ataccggagg gacattttcc gatttcgttt
      961 atctgaatga agaaaccggc gagctgtcga tcgccaagct gccatcgacg cccgatgatc
     1021 cctcgcgcgc gatcctagcc ggcatcgagg cgctcttggc aacggcgggc gaaggcgccg
     1081 acatcggcta tttctgccac ggcaccacgg tcgggaccaa cgcgttgctc gaaggcaagg
     1141 gcgtcaggac cggcctgttg gtgacggagg gatttcgcgg catctacctc gtgggggagc
     1201 aggcgcggcc gtacggcacc gcgatcttcg acgtcatgta cgacaagcct gcgccactgg
     1261 tgccgccgag cctgaccggc gaagtgaagg agcgcgtcga cttccgcggc aatgtgctgc
     1321 gcgacctcga cgagacggcg ctgcgcgaaa ccgtgcgcga gctcacggcg cggaacgtcg
     1381 agtcgatcgc ggtgtgcctg ttgttctcgt ttctgcaccc cgcgcatgaa gcgcgcgtgc
     1441 gcgaaatcat cgttgaggaa atgccggatt gcagcatctc gctgtcgtcc gaggtgctgc
     1501 cgcagatccg cgagtattat cggctgagca ccaccgtcat caacgcctat ctgcagccga
     1561 tcctcgcgcg ctatatcgcg caactc
//
LOCUS       DATLJT010001718         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3638885,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001718 DATLJT010000000
VERSION     DATLJT010001718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3638885"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VM910_39095"
     CDS             complement(<1..576)
                     /locus_tag="VM910_39095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI68538.1"
                     /translation="MTMSVELPLEQNEATAPQAGTAAQEGARPTALRRYARPALGLVL
                     PVGAALAWELIVWLGLSSGRLVPPPSRVFETLVELARSGELLRHIVATLTRVALGFNF
                     GVIAGTVLGAISGYWGLARRLLDPTVQALRAIPSLAWVPLFILWLGIFETSKVALIAV
                     GVFFPVYLGVLGAILSVDRKIVEVGRAFRLSG"
     gene            complement(784..1761)
                     /locus_tag="VM910_39100"
     CDS             complement(784..1761)
                     /locus_tag="VM910_39100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HVI68539.1"
                     /translation="MTAGISRRALALLSAMWVAMPGAATAAEALKEIHIDWATYNPVS
                     MVLKQNGLLEKEFAKDGIGIVWVQSAGSNKALEFLNAGSIDFGSTAGSAALVARINGN
                     PIKSIYVYSRPEWTALVTTKDSKIATVADLKGKRVAVTRGTDPHIFLVRALLGAGLSE
                     KDITPVLLQHADGKTALIRGDVDAWAGLDPMMAQAEIEDGAKLFYRKADANTWGILNV
                     REQFLKDYPDIVRRVLVTYEEARKYSLAHYDELKKTFMSVTKLPENVVDKQLKERTEL
                     THSRIGTPQRESILAAGLALQQAGVIDSKVDVKANLDALIDDQVPLPTN"
     gene            1963..>2400
                     /locus_tag="VM910_39105"
     CDS             1963..>2400
                     /locus_tag="VM910_39105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HVI68540.1"
                     /translation="MDSIVTQPQHETDAVFDTARITAAIDALAEKHVGREDAFRSAVA
                     QLLKAELIDARDAAQKVLLRDRHGRRCAERLCFVQDEIIRILFAAATRHLYRSHVPSG
                     AERMAIVATGGYGRGLMAPESDIDLLFILPYKQTAWGEQVAEAI"
BASE COUNT          438 a          799 c          743 g          420 t
ORIGIN      
        1 tcccgacagg cggaaggcgc ggccgacctc gacgatcttg cgatcgacgg agaggatcgc
       61 gccgagcacg ccgagataaa ccggaaagaa cacgccgacc gcgatcagcg cgactttcga
      121 ggtctcgaag atgccgagcc acaggatgaa cagcggcacc caggcgagcg aaggaattgc
      181 gcgcagcgcc tgtaccgtcg gatcgagcag gcgccgggca agcccccaat agccggagat
      241 cgcgcccagc acggtgcccg cgatcacccc aaaattgaag cccagggcca cgcgcgtcag
      301 cgtcgcaacg atgtggcgca gcaactcgcc gcttctcgcg agctcgacga gcgtctcaaa
      361 cactcttgac ggcggcggca ccagccgtcc gctggagagg ccgagccaca cgataagctc
      421 ccaggcgagc gcggccccga ccggcaacac gagcccgagc gccgggcgcg cataacggcg
      481 caaggcggtc gggcgcgcgc cctcttgtgc agccgtgccg gcttgcggcg ccgtcgcttc
      541 gttttgctca aggggcagtt cgaccgacat ggtcatagca agacgcttct cggcagagga
      601 ttgcaaggtg ctcaccctcc cctggagggg gagggtcggc tcacatgagc gtagcgaaat
      661 gtgagaccgg gtggggtgcg cggtaggcga caagtttcac cccaccccgc cgctcacgtt
      721 gttcgcgggc gaccctcccc ctccagggga gggtaagaaa gcgcgcacgt gtttcacacg
      781 ttatcaattc gtcggcagcg gaacctgatc gtcaatcagc gcgtccaggt tcgccttcac
      841 gtcgaccttg gaatcgatca cgccggcctg ctgcagcgcc agccccgcgg ccaggatcga
      901 ctcgcgctga ggcgtgccga tgcggctgtg ggtgagctcc gtgcgttcct tgagctgctt
      961 gtcgaccacg ttttctggca gcttggtcac gctcatgaag gttttcttca gctcgtcgta
     1021 atgggccagc gagtatttgc gcgcctcctc ataggtcacg agcacgcggc ggacgatgtc
     1081 cgggtaatcc ttcaggaact gctcgcgcac gttgaggatg ccccaggtgt tggcgtcggc
     1141 cttgcgatag aacagtttcg cgccgtcctc gatctcggcc tgcgccatca tcgggtcgag
     1201 cccggcccag gcgtcgacgt cgccgcggat cagcgcggtc ttgccgtcgg cgtgctgcag
     1261 cagcaccggc gtgatgtcct tctccgacag ccccgcgccg agcagcgcgc gcaccaggaa
     1321 gatgtgcgga tcggtgccgc gcgtcaccgc gacacgcttg cccttcagat ccgcaacggt
     1381 tgcgattttg gaatctttgg tcgtcaccag cgccgtccat tcggggcgcg aatagacata
     1441 gatcgacttg atcggattgc cgttgatgcg cgcgaccagc gccgcggaac ccgccgtcga
     1501 accgaaatcg atcgagcccg cgttgaggaa ctccagtgcc ttgttggacc ctgccgactg
     1561 cacccagaca attccgatgc cgtccttggc gaactccttc tccaggaggc cgttttgctt
     1621 cagcaccatc gacaccggat tgtaggttgc ccagtcgatg tggatttcct tgagcgcttc
     1681 tgccgcggtc gccgcgcccg gcatcgcgac ccacatcgcc gagagcagcg ccaatgcgcg
     1741 tcttgaaatt cctgccgtca ttactcacct cctccgaagc ctaaaaaatc aaccgttatt
     1801 tcaatgcgtt acctcccgcg gtcaacaaga aaatccgcac gctcgaagcc gcggctgcga
     1861 gaacgatttt gcttgccgcc gggcaaacgc agcatgggcc tcctatgtat tattttacaa
     1921 tgtcgggtga cagcacgacc cccgtccgat accgattagc ccatggacag tatcgtcacg
     1981 cagccacagc acgaaaccga cgcggttttc gacaccgccc ggattacggc ggcgatcgac
     2041 gcgctggctg aaaagcacgt gggccgcgag gacgcgttcc gctctgccgt ggcgcagctc
     2101 ctgaaggccg aactgatcga cgcgcgcgat gctgcgcaaa aggtgctgct gagggatcgc
     2161 cacggccgcc gctgcgccga gcggctctgc ttcgtgcagg acgagatcat ccgcatcctg
     2221 tttgcggcgg ccacacgaca tctctaccgc tcccatgttc cctccggcgc cgagcgcatg
     2281 gcgatcgtgg cgaccggcgg ctacgggcgc ggcctgatgg cgcccgaatc cgacatcgat
     2341 ctgttgttca tcctgcccta caagcagacc gcctggggcg agcaggtggc cgaggcgatc
//
LOCUS       DATLJT010001719         3500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1340716,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001719 DATLJT010000000
VERSION     DATLJT010001719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340716"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..641
                     /locus_tag="VM910_39110"
     CDS             213..641
                     /locus_tag="VM910_39110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68541.1"
                     /translation="MPNFTALDMSLPEVAILVAAMVQARDAVGRRWWSHPQEPTGEEW
                     WADVLADPRARRRERRTDLAVKQAFHRLADEKRDIILVACSKCDWRAAFSRDELIASH
                     GADYAMPNLLDHLAAPSCSRLGSNWDRCGVHYVEPIEGKR"
     gene            695..1204
                     /locus_tag="VM910_39115"
     CDS             695..1204
                     /locus_tag="VM910_39115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI68542.1"
                     /translation="MEGGKRADRPELAKALAACRKHKAKLIIAKLDRLSRNLAFIATL
                     MDSGVEFIAVDNPHANKLTIHILAAVAQHERETISERTKAALAAAKRRGVKLGSPRLV
                     AARKASRKARSTAADTFAANVRPIIKEIQNSGVSSLRGVAKALNARGIRTARGSEWTG
                     VQVSNVLER"
     gene            1239..2084
                     /locus_tag="VM910_39120"
     CDS             1239..2084
                     /locus_tag="VM910_39120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68543.1"
                     /translation="MTLRAPEPFSKFNSDPLDVGARSESAHSSHNLHSGYVAVPVTEE
                     PGPDNLNGFPLRQTLRQRHTIRPQREIGLTAHLRRVIIAPKASPSRYAGIPGFDVGTD
                     DAPTPSTPPPERSLVENHELVTDLARYSETLCSESAIRKKWRLSEDTWKMLGSSDELV
                     RAIEEEKTRRIRSGATKRELAQQHIIRGPGVLASIMDDPQANARHRVDSIKTLNAIAD
                     PGPEAAVGEERIIIKIDMGADLRAKGQEPNPGDVVIIEAAARPSTPKQIENDHSEQSD
                     DEWRR"
     gene            2072..2383
                     /locus_tag="VM910_39125"
     CDS             2072..2383
                     /locus_tag="VM910_39125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68544.1"
                     /translation="MATMTIFERLNQGRPPAEMAIKQSSKAPAQLLLDWLPRWPQDTI
                     TVRQIRVYGPYTIRNRRSAIDAAEVLVTNGWLKPIKPSRPDTYAWQITRKTILHPPVA
                     M"
     gene            complement(2768..>3500)
                     /locus_tag="VM910_39130"
     CDS             complement(2768..>3500)
                     /locus_tag="VM910_39130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HVI68545.1"
                     /translation="MLENLQMARPGQQMDDGDDDMMSALDELGDMIRKQQQLRDRTFQ
                     QGQDQRRDRQRGQRGQQGQQGQQGQQGDQMGELRENQQALRDQLKKLMEELRKKGFGQ
                     GQQGQGEMDQLGRAGDAMGEAEGQLGEGNADGAVDSQGRALEALRRGAQNLAQSMQQQ
                     GMLGPGPNGQPGRLGPPRARQETDPLGRPLRGRDYGDDTTVKVPGEIDVQRARRILEE
                     LRRRFGENFRPQLELEYIERLLRDF"
BASE COUNT          719 a         1147 c         1032 g          602 t
ORIGIN      
        1 cgagcctgag gcccgtcttt ggtcttcctt gccaaaccgc gaacctgtgg cgcatcaaaa
       61 tcctggcgca atggtgtcgg agctgacatg acgagcctcc tgggttcgcc atgttgaatc
      121 tgactcgcct ccatctggga accgcccacg agtcacaatt tcagggactc ggtataagac
      181 ccaattgatg ttgtcacccg agaaggccgc agatgccgaa cttcaccgcg ctcgatatga
      241 gccttcccga ggtcgcaatc ctagtggcgg cgatggtgca ggcgcgtgac gccgtgggcc
      301 gtcggtggtg gtcgcacccg caagagccaa cgggcgaaga atggtgggcc gacgtgcttg
      361 cggaccctcg cgctcgaagg cgagagcggc gcaccgatct agcggtcaag caagcgttcc
      421 atcggctcgc cgacgaaaag cgcgatatca tacttgtagc gtgcagcaag tgcgattggc
      481 gagctgcatt ttcacgcgac gagctgatcg cctctcatgg cgcggactac gcgatgccca
      541 acctactcga ccatctcgcc gcaccgagtt gcagcaggct cggctccaat tgggatcggt
      601 gcggggtgca ttacgtcgag ccgatcgagg gcaagcggta gctcttttgg gtggcacggc
      661 cgacgcaaca cactttcgga gcgatgacgt cggggtggag ggcggcaaac gcgcagaccg
      721 ccccgaactg gccaaggcac tggccgcctg ccgcaagcac aaggccaagc tcatcatcgc
      781 caagctcgac cggctgtccc gcaacctcgc cttcatcgcc acgctgatgg acagcggcgt
      841 cgagttcatc gccgtcgata acccgcacgc caacaagctc accatccaca tcctggccgc
      901 cgtcgcgcag cacgaacgtg aaacgatcag cgagcgcacc aaggctgccc tcgcggctgc
      961 caagcgccgc ggcgtcaagc tcggcagtcc gcgcctcgtc gccgcccgca aggcgtcgag
     1021 aaaagcccgt agcacggccg ccgatacgtt cgccgccaat gtccggccca ttatcaagga
     1081 aattcagaac agcggcgtca gcagcctccg cggcgtcgcc aaggcgctaa acgcccgcgg
     1141 tatacggaca gctcgcggta gcgagtggac cggagtgcaa gtgagcaacg tcctcgagcg
     1201 ttagccgaca ggggacccga actgcctgcg gaaccaaatt gacgctgcgc gcgcctgagc
     1261 cgttttccaa attcaactcg gatccgctcg acgtcggtgc ccggagcgag agcgcgcact
     1321 cgagccacaa cctccacagc gggtatgtcg cggttcctgt tacggaggaa ccaggcccag
     1381 acaacttgaa tggcttccct ctccgacaaa cgctgcgcca gcgccatacc atccgccctc
     1441 agcgcgaaat cggcctgact gcgcacctac ggcgcgttat aatcgcgccc aaggcaagcc
     1501 ccagcagata tgccggcatc ccgggcttcg acgtcggaac cgacgacgct ccgacaccga
     1561 gtactccacc gccggaacgc tcgctcgtcg agaaccacga actggtaacc gatctcgcga
     1621 gatattctga aacgctatgc tccgagtccg ccatcagaaa gaagtggcgg ctcagcgagg
     1681 acacatggaa aatgctcggc agcagcgacg agctggtccg agcaatcgag gaagaaaaga
     1741 cgcggcgaat ccgctcggga gcaacaaagc gtgagctcgc gcagcagcac atcattcgcg
     1801 gacccggcgt cttagcctcg atcatggacg acccccaggc aaatgcgcga caccgggtcg
     1861 acagcatcaa aacgctgaac gcaatcgctg accccggacc agaagccgca gtaggggaag
     1921 aaagaattat catcaaaatt gacatgggcg cagatttgag agccaaaggt caggaaccaa
     1981 atccgggcga tgttgtgata atcgaagcgg cggcgcggcc cagcaccccg aaacaaatcg
     2041 aaaatgatca cagcgagcaa tcggacgacg aatggcgacg atgacaattt ttgagcggtt
     2101 aaatcaggga cggccaccgg ccgaaatggc gatcaaacaa tcgtccaaag cgcccgcaca
     2161 gctcctgctc gattggctac ctcggtggcc ccaagatacg atcaccgtcc gccaaatccg
     2221 cgtctatggc ccctacacca tccgaaaccg tagaagcgcg atcgacgcag ccgaagtcct
     2281 ggttacgaat ggctggctaa aaccaatcaa accgtcccgg cccgacacct atgcgtggca
     2341 aatcacccgg aaaaccatcc ttcatccccc tgtagcgatg tagctggaat attccgaaaa
     2401 atactccggc ggattatttt ttggggcaag gaaattgagc ctgcgccttt actcgctcac
     2461 gaggccttcg aagtcgccca atgggccagt caatcctcag ctgcggcggc ggtgcagcag
     2521 atgggcacgc gattggcgtc tggcggcgga acgctcgcta cccttgtgcg ccaaaatcaa
     2581 gacctcaaac ctactactac ggctaccctg tctgacggtc gaggaccttg aggcaattgc
     2641 aggtgcagtg ggcgtcggcc cctcagggcg catcacctgg catagctact accgcgccgc
     2701 cgcccgcgag ctcgcgccgg agggggaggg ggtggcaata ggatggaaga gttctgccgt
     2761 ctgatttcta gaaatcccgc agcagccgct cgatgtattc gagctcgagc tgcgggcgga
     2821 agttctcgcc gaagcgccgg cgcaattctt ccagaatgcg ccgcgcacgc tgcacgtcga
     2881 tttcgcctgg taccttgacc gtcgtgtcgt cgccgtagtc gcggccgcgc aagggtcggc
     2941 cgagcgggtc agtctcctgt cgggcacgcg gtggcccgag cctgccgggc tgtccgttag
     3001 ggccgggacc caacatgccc tgctgctgca tcgattgggc gaggttctgc gcgccgcggc
     3061 gcaaagcttc gagcgcgcgg ccttgggaat cgaccgcgcc gtcggcgttg ccttctccaa
     3121 gctgaccttc cgcctccccc atggcgtcgc cggcgcggcc gagctgatcc atctcgccct
     3181 gcccttgctg gccttggccg aagcccttct tgcgcaactc ctccatcaat tttttgagct
     3241 gatcgcgcaa tgcctgctga ttctcgcgca gctcgcccat ctgatcgccc tgctggcctt
     3301 gctgcccttg ttggccctgc tgaccgcgct gcccgcgttg gcgatcgcgc cgctggtcct
     3361 ggccctgctg gaacgtgcga tcgcgcaatt gctgctgctt gcggatcatg tcgccgagct
     3421 cgtcgagcgc cgacatcatg tcatcgtcgc cgtcatccat ctgctgcccg ggacgagcca
     3481 tctgcaggtt ttccagcatc
//
LOCUS       DATLJT010001720         2405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2487674,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001720 DATLJT010000000
VERSION     DATLJT010001720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2487674"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..564
                     /locus_tag="VM910_39135"
     CDS             166..564
                     /locus_tag="VM910_39135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68546.1"
                     /translation="MILPPALLLGVSLAIPVVDKVPALNVEQVCEGIAQQGGVSFHDP
                     NIAVERKNCLDSEHAIRDEISKQWSSFSASDKTSCTNEATMGGESSYTELLTCLEMAR
                     DVRALHNDQQKQVDTGPIPTAPPGHAQPKP"
     gene            complement(607..1404)
                     /locus_tag="VM910_39140"
     CDS             complement(607..1404)
                     /locus_tag="VM910_39140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68547.1"
                     /translation="MRGGWYGGCLKMALAVGFGASLAMAGNDVSAAAQRQINQVNPDL
                     DTDDQLAPSQMKQPVPAAVSHPGSAAHASAPAAVRHAGPAPAGGGHTVACSGTFGKDS
                     SHLKLAMTFDSKNITFTDVESTGGTKVPASVLFPKDPKRRLEVWWANPAARSDTYLIV
                     INGKSDWSAPGGLRLGLNLAQLEKLNHKAFKLKGFDKDGTATVSDWDGGVLASLPGGC
                     KSGVNLRADPKVPAETVAALAADKEYSSEDPEMRAVKPAVSEILIGY"
     gene            1644..>2405
                     /locus_tag="VM910_39145"
     CDS             1644..>2405
                     /locus_tag="VM910_39145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HVI68548.1"
                     /translation="MRKHRVDAGPDTVHWGFFDAGLNPLITIDPGDEVTISTVSGPPE
                     AMPKAESGLAVPPALSAIHAKVQQRLGPHIMTGPIAVRGAKAGQVLEVRIKSIALHYD
                     WGYNVIRPLAGALPDDFASTRLIHIPLDRTRMTGRLPWGLELQLKPFFGVMVVAPPRA
                     WGPVGSPPPRRNGGNMDNKELVAGSTLYLPVHVDGALFSVGDGHGVQGDGEVCVTAIE
                     TGLIGTFELHIRDDFALEWPMVETPNELITMGFDPD"
BASE COUNT          451 a          772 c          727 g          455 t
ORIGIN      
        1 gccgaatagc ggtgcgagaa tcattggctg cttcccttac cctcgcgcgc aggcatagcg
       61 ggcgcacctg ccgccggtca accctgatct cgcgccggca tcattgtgtt ttttgcacca
      121 aggtgatgtt atattcggca atcaacggaa tgacggagtt tggccatgat attgccgccg
      181 gcactgcttc ttggcgtcag ccttgccatt cccgtcgtcg acaaagttcc agcgctgaac
      241 gtggaacaag tctgtgaagg catcgcacaa cagggtggcg tctcattcca cgatccgaat
      301 attgcggtgg agaggaaaaa ttgcctcgac agcgagcacg cgattcgcga cgagatctcc
      361 aagcaatggt cgagtttttc ggcgagcgat aagacctcgt gcacgaacga ggcgacgatg
      421 ggtggcgagt cgagctacac cgagctgtta acctgtctgg aaatggcgcg cgacgttcgg
      481 gcgttgcaca acgaccaaca aaagcaggtg gacaccggcc ctattccgac ggccccgccc
      541 ggtcacgccc agcccaagcc ttgatcgagt aaagtcgcaa tcgcggaatg gcctgatcgg
      601 cgaacctcaa tagccgatca ggatttcgct caccgccggt ttgaccgccc gcatctcggg
      661 atcttccgag ctgtattcct tgtcggcggc gagagcggcg acggtctcgg cgggcacttt
      721 cggatccgcg cgcaggttca ctcccgattt gcagccgccg ggcaagctgg ccagtacgcc
      781 gccgtcccaa tcgctcacag tggcggtgcc gtctttgtcg aagcctttga gcttgaaagc
      841 cttgtgattg agtttctcaa gctgcgcgag gttgagcccc agccgcaatc cgccgggcgc
      901 gctccagtcg gacttgccgt tgatgacgat cagataagtg tcactgcgcg ctgcgggatt
      961 ggcccaccag acctcgagcc gccgcttcgg atctttcgga aacagcaccg atgccggcac
     1021 cttcgtgcct ccggtggatt cgacatcggt gaaagtgatg ttcttggaat caaaggtcat
     1081 cgccagcttg agatggctcg aatccttgcc gaaggtgccg ctgcaagcca cggtgtgccc
     1141 gccgccggcc ggcgccggac ccgcatgcct caccgcggcc ggcgccgatg catgcgcggc
     1201 gcttcccggg tgcgacaccg ccgccggaac cggctgcttc atctgtgacg gcgcgagttg
     1261 gtcgtcggtg tcgagatcag gattgacttg attgatctgg cgctgcgccg ccgccgagac
     1321 gtcattgcca gccattgcga gtgacgcccc aaaacccacg gccagtgcca ttttcaggca
     1381 gccgccatac catcccccac gcatactcgc ctcccgttct gggtgaaccc tacaatttca
     1441 agcttttaag gcccgcactg cagccggcct cgccagcatt gcccggatgc agaatagcac
     1501 gccgcggctg gttcggcacg gcccggcctt tgccactatc tagccacccg caccgtctgg
     1561 actagcacgc ctgtttcctg acccaagatt ctggcagaac cgaagcaaaa atcggcaggc
     1621 ttctcccatt ccaaggggca caaatgcgca agcatcgcgt ggacgcagga ccggatacgg
     1681 ttcactgggg cttttttgac gcagggctga acccgctgat cacgatcgac cccggcgatg
     1741 aagtcacaat ctcgaccgtc tcggggccgc ccgaagcgat gccgaaagct gaaagcgggc
     1801 tagctgtccc gccggccttg tcggccatac acgcgaaagt gcagcagcgg cttggtccgc
     1861 acatcatgac cggtccgatc gcggtgcgcg gcgccaaagc cgggcaggtg ctagaggtga
     1921 ggatcaaatc gatcgcgctg cactatgact ggggctacaa cgtcatccgc ccgcttgccg
     1981 gcgcgctgcc cgacgatttt gcctcgacgc gtctcattca tattccgctc gatcgcacgc
     2041 ggatgaccgg ccggctgccg tggggcctcg aactgcagct caagccattc ttcggcgtca
     2101 tggtggtggc gccgccacgc gcctggggac cggtcggctc gccgcccccg cggaggaacg
     2161 gcggcaatat ggacaacaag gaactcgtcg ccggcagcac gctttacctg ccggtccatg
     2221 tcgacggtgc gctgttctcg gtcggcgacg gccacggcgt gcagggcgac ggcgaggtct
     2281 gcgtcacggc gatcgagacc gggctgatcg gcaccttcga attgcacatc cgcgacgact
     2341 tcgcactgga atggccgatg gtggaaacgc cgaacgagct cattaccatg ggattcgatc
     2401 ccgat
//
LOCUS       DATLJT010001721         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_877546,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001721 DATLJT010000000
VERSION     DATLJT010001721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877546"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..684
                     /gene="maiA"
                     /locus_tag="VM910_39150"
     CDS             40..684
                     /gene="maiA"
                     /locus_tag="VM910_39150"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HVI68549.1"
                     /translation="MSRDLYTFFRSSTSYRLRIALAYKQLEYTPHYVSLPKMEHRAPS
                     YRALHAQGLVPLLIDGDRSFIQSMAIIEYLDEVYPEPPLMPADVRGRHYVRAVSQIIG
                     CDIHPLNNVRVLKHIQTQFGADDAATKTWYRHWIAEGLGGLETYVASAGFARRFCYGD
                     TVTMADVCLVPQIFNAQRFDCPLDEYGTLTAIFERCMAVDAFRTTQPNTQADAF"
     gene            complement(737..1213)
                     /locus_tag="VM910_39155"
     CDS             complement(737..1213)
                     /locus_tag="VM910_39155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI68550.1"
                     /translation="MRTDTVHSDMPLWGRPGYLLRRLHQIHYALFFEECAGFDITPVQ
                     YGLLTTLSLNPDLDQNSLARELGIDRTNVADVLNRLARRGLLERRRSADDGRMVLARL
                     TRPGERLTRKMYWPMRRAQKRLLEPLLSQERNALIITLIRLINGNNHLGRTIFQPS"
     gene            1498..>2364
                     /gene="dctP"
                     /locus_tag="VM910_39160"
     CDS             1498..>2364
                     /gene="dctP"
                     /locus_tag="VM910_39160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HVI68551.1"
                     /translation="MQRRAFLMRASAGVAATAIARPAIAQTGAPVRWRLATSWPKSLD
                     TLYGSVDAMCQRVGQMTDRKFQIQCFAGGEIVPPLQVWDATQNGTVECGHTLTSFNIG
                     KNPAVAFDSGLAFGLNTRQQQAWMNYGGGLELIRALFKKDGILPIPCGNVGVQMGGFY
                     RKEINSVDDLKGLKFRIGGLGGMILAKLGVVPLQIPTADIYPSLERGAIDAAEWIGPY
                     DDEKLGLHKVARYYYYPGWWEGSAQVTLLVNLQHWEALPESYRAALEAACAEATVLMM
                     AKYDTRNPEALRR"
BASE COUNT          460 a          711 c          716 g          477 t
ORIGIN      
        1 acgagattct caacaaagcc gccgcggcgg agtagacgtt tgtcgcggga tttatatacg
       61 ttcttccgat cctccacctc ataccggctt cgcattgcgc tcgcctacaa gcagctcgag
      121 tacacgccgc actacgtcag cctgccaaaa atggagcacc gagcgccctc atatcgggcg
      181 ctgcatgctc agggattggt gcccttgctg atcgatggcg atcgatcctt cattcagtcc
      241 atggccatca tcgagtatct ggacgaggtc tacccggagc ctccgctgat gccggcggac
      301 gtgcgtggac gccactacgt gcgagcggtg tcgcaaatca tcggctgtga tattcaccct
      361 cttaataacg tacgcgtgct caagcacatc cagacgcagt tcggtgccga cgacgcagcg
      421 accaaaacgt ggtatcgcca ttggatcgct gagggattgg gaggtctgga gacttacgtg
      481 gctagcgcgg gcttcgcccg tcgcttctgc tatggcgata ccgtcaccat ggccgacgtt
      541 tgcctcgtgc cgcagatttt caatgcccag cgcttcgatt gtccgctcga cgagtacggc
      601 accctgactg cgatcttcga gcgatgcatg gccgtcgacg cctttcgcac gacccagccc
      661 aacactcagg ccgacgcatt ctgaaccgga ctgcgccgtg ggacgggcaa ggctacccgt
      721 ctgcgatcat tcgatctcaa ctgggctgaa agatcgtccg gccaagatga ttgttgccgt
      781 tgatgaggcg gatcagggtt atgatgagcg cgttgcgctc ctgcgagagg agcggctcga
      841 gcagacgctt ctgggcgcgt cgcatcggcc aatacatctt tcgcgtcagt cgctcgccgg
      901 ggcgggtcaa tcgcgcaagg accatgcgtc cgtcgtccgc gctgcggcgg cgctcgagga
      961 gcccgcgtcg cgcgaggcgg ttgaggacat cggcgacatt ggtgcggtcg attccgagtt
     1021 cgcgcgccaa tgagttttga tcgagatcgg gattgagcga tagcgtcgtc aacaatccgt
     1081 actgaaccgg tgtgatgtcg aatcccgcgc actcctcgaa aaaaagcgcg taatggattt
     1141 gatgcaagcg ccgcagaaga taacccggcc gaccccacag cggcatgtcg gaatgaaccg
     1201 tgtcagtacg caacggtctt ttcttggcgt tgcgtcggcg cgctttcctt tcactggcga
     1261 gtcttggtgc gtgtcttcgt gccgctttca ttcttgtcgc catttgacac tcctcctcgt
     1321 cgcactaggc tcgcttcccg cacgacagca aatattcagt atagggtcat atttgcaggc
     1381 actggagact atgttaggct tttatccacg ctgcgcgcag atacaaccag caaaaaagaa
     1441 actgcgactc cgaaggccgc gccggttctc ccagtcatat ctcggcagga gacaaaaatg
     1501 caaaggcgtg cgtttctcat gcgagcgagc gcgggcgtcg ccgcgaccgc gatcgcaagg
     1561 ccggcgatcg cgcaaaccgg cgccccggtg cgttggcggc tcgcgacgag ctggccaaag
     1621 agtctcgata cgctttacgg cagcgtcgac gccatgtgcc aacgtgtggg gcagatgacc
     1681 gacaggaagt tccagattca gtgcttcgct ggcggtgaga tcgtgccgcc gctccaagtt
     1741 tgggatgcga cgcaaaacgg caccgtcgag tgtggccaca cgctcacctc gttcaatata
     1801 ggcaagaacc ccgcggtcgc gttcgattcc gggctcgcgt tcgggctcaa cacgcgccag
     1861 cagcaggcct ggatgaatta cggcggaggg ctggagctca tccgcgcgtt gttcaagaag
     1921 gacggcatcc tgcccattcc ctgcggcaat gtgggcgtgc agatgggtgg cttctatcgc
     1981 aaggagatca actccgtcga cgacctcaaa gggctcaagt tccgcatcgg cgggctaggt
     2041 ggcatgatct tggcaaagct cggcgttgtg ccgctgcaaa ttcccacggc cgacatttat
     2101 ccatcgctcg aacgcggcgc aatcgatgcc gccgagtgga tcggccctta cgacgacgag
     2161 aagctcgggc tgcacaaggt cgccaggtac tactactatc ccggctggtg ggagggcagc
     2221 gcccaggtca cgctgctcgt caatctccag cattgggagg ctctgcccga gtcctatcga
     2281 gccgcgctcg aggcggcatg cgccgaggcg accgttttga tgatggcgaa gtacgacacc
     2341 agaaatcccg aggcgttgcg ccgg
//
LOCUS       DATLJT010001722         1562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1569453,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001722 DATLJT010000000
VERSION     DATLJT010001722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569453"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VM910_39165"
     CDS             <1..583
                     /locus_tag="VM910_39165"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVI68552.1"
                     /translation="RVFLERGLAGASMDEIAGRACAGKPTIYARFPSKEALFTAVVMQ
                     SVAANMARYEAYTPTGATIEDRLESVAATVLQWMLVSDAIRLMRVAIAEAPRFPDLAS
                     SVYGMARNRATQAVARLLAEAAQSDELGTLPAFAPTRVKTTTRFFQDLVVFPLAMRAL
                     FGEQLKELRAEIAPHVRGSVAFFLAACRHGGVI"
     gene            complement(611..1069)
                     /locus_tag="VM910_39170"
     CDS             complement(611..1069)
                     /locus_tag="VM910_39170"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVI68553.1"
                     /translation="MKAKTRNELIAQCACFDLRKATRAVSRMYDDFLRDEGLNITQFS
                     LLRLIRTEKELSISTLGRYMVMDRTSITRALAPLERDGLIDSRPGADKRIRIVSVTNK
                     GRKLVEEAEPRWRQAQEALIETIGEDRWRAMCALLRDTTRMVRHRTVTAA"
     gene            1231..>1562
                     /locus_tag="VM910_39175"
     CDS             1231..>1562
                     /locus_tag="VM910_39175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HVI68554.1"
                     /translation="MTSQRSVVFAEPVRTAIGTFGGSLKDVPAPSLGAVAIGAAVARA
                     GIRAEDVETVVMGNVVQAGAKMNPARQAAVHAGLPVTIPALTVNRVCGSGAQAIVSAA
                     QEISLGNI"
BASE COUNT          278 a          488 c          487 g          309 t
ORIGIN      
        1 ccgcgtcttt ctcgaacgcg ggctggccgg cgccagcatg gatgagatcg ccgggcgtgc
       61 ctgcgccggc aagccgacca tctacgcccg ctttcccagc aaggaggcgc ttttcaccgc
      121 cgtggtgatg cagagcgttg ctgccaacat ggcgcggtac gaggcctata cgccaaccgg
      181 tgccacgatc gaagatcgcc tcgagagcgt cgccgccacc gtcctgcaat ggatgctggt
      241 cagtgacgca atccgtttga tgcgggtggc gattgcggag gcgccgcggt ttcccgactt
      301 ggccagcagc gtctatggca tggcgcgcaa ccgcgcgacg caagccgtcg cccggcttct
      361 ggccgaggca gcccaatccg acgagctcgg cacgttaccg gcgtttgctc ccacgcgcgt
      421 gaaaacaacc acgcgatttt tccaggattt ggtcgtgttt cccctggcga tgcgagcgtt
      481 gttcggcgag cagctcaaag agctgcgtgc ggagatcgca ccgcacgtaa gagggagcgt
      541 tgcgttcttt cttgccgcct gtcggcacgg cggcgttatt tgagtcgctt aagtcactac
      601 gctattgcgg ttaggccgcc gtgactgtgc ggtgccgcac catacgggtg gtgtcccgca
      661 acagcgcgca catcgcacgc caacggtctt cgccgatggt ctcgatcagc gcctcctgtg
      721 cttggcgcca cctcggttcg gcttcctcga cgagcttgcg gcccttgttc gtgacggaaa
      781 cgatacgaat ccgcttgtca gcgccgggcc gactgtcgat cagtccgtcc cgctccaagg
      841 gagcaagcgc gcgggtaatg ctagtccggt ccatgaccat gtaccgaccg agcgtcgaaa
      901 tcgacagctc cttttccgtg cggatcaggc gaagcagtga gaactgcgtg atgttgagcc
      961 cttcatcccg caggaagtcg tcgtacatgc gcgagaccgc gcgcgttgcc ttgcgcaggt
     1021 cgaaacacgc gcattgcgcg atcaattcat tgcgtgtctt tgctttcatc cgcattcccg
     1081 cttcttccgg ccaagactcc ggcaggtaaa actcacaaaa aagtgactat acacgtagtg
     1141 cacacgtatt gtgtcgcaat ataggtgtgt atacactcat atgtcaagac gatgcagaaa
     1201 tgcatcgaac gtcgttcggg aggttttgtc atgacatcgc agcggtcagt tgtgttcgca
     1261 gaaccggttc gcaccgcgat cggcaccttt ggcggcagcc tcaaggacgt gccggctccc
     1321 agtctcggcg cggtggcgat cggcgccgcg gttgcccgcg cgggaatccg cgcggaagac
     1381 gtggaaacgg tcgtcatggg caacgtcgtg caggccggag ccaagatgaa tcccgcccgg
     1441 caagcggccg tccatgccgg gctgccggtg acgatcccgg cgctcacggt caatcgcgtt
     1501 tgcggctcgg gagcgcaggc catcgtctcg gcggcgcagg agatctccct cggcaacatc
     1561 aa
//
LOCUS       DATLJT010001723         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1108434,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001723 DATLJT010000000
VERSION     DATLJT010001723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108434"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VM910_39180"
     CDS             complement(<1..521)
                     /locus_tag="VM910_39180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI68555.1"
                     /translation="MEAFGLLLHGFTVLLTWKTLAVMMVGLVLGIFVGVLPGLGGPNG
                     VAILLPLTFTMDPTSAIVMLSCIYWGALFGGAITSILFNIPGEAWSVATTFDGYPMAQ
                     HGQAAEALTAAFTSSFIGSLVAVLLITFLAPGIAAFALQFGPPEFFAVYLLTFCSFVG
                     LGREDKHKTVISMT"
     gene            complement(697..1239)
                     /locus_tag="VM910_39185"
     CDS             complement(697..1239)
                     /locus_tag="VM910_39185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HVI68556.1"
                     /translation="MATPETEIVVEDPTAPSDNSPSVVGTRTVDIVVSLLLLALAALL
                     CFDNWRTGIAWAADGPQAGYFPFYLGLILAAASLYGLITSLVTGAGATQTFLTRDQLR
                     RVMQVFVPTLLFCLLTQWLGLYVASFLLIAGFMRIIGRIALWKSILTAFLFAIIMFVT
                     FDIAFDVIMPKGPLEAAFGY"
     gene            complement(1372..>2284)
                     /locus_tag="VM910_39190"
     CDS             complement(1372..>2284)
                     /locus_tag="VM910_39190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68557.1"
                     /translation="NKPVEIVVAAGAGGASDQMARMMQAAIQKNNLMKQPMVVSLKGG
                     ASGAEALIYMKSNEGDPNKVLIAYSLIYMLPLSAKIPFNWRDLTPVSVIALDQFVLWV
                     NTQLPYKTVKEFTDAAKSAAPPLKMGGTGSRREDHVLTVFLEQKTGAKFAYLPYKSGG
                     EAATQLVGNHTASNVNNPSENLEVWRAGQVRPLCVFDKERIEYKAKVISDMSWNDIPT
                     CKEQDLDIQYLMLRAMFLPGKVTPEQTAFYVDLFKKLTQTAEYKDYMEKQALKPIFLT
                     GNDMIKFLEEDDALNKNLMTEAGFVAN"
BASE COUNT          475 a          650 c          711 g          448 t
ORIGIN      
        1 gtcatggaaa tgacggtctt gtgcttgtcc tcgcggccga ggccgacgaa cgagcagaac
       61 gtcaatagat agacggcgaa gaactcgggt gggccgaact gtagtgcgaa cgcggcaata
      121 cccggtgcaa gaaacgtgat cagcaggaca gccaccagcg agccgatgaa ggacgaagtg
      181 aaggccgcgg tcagcgcctc ggccgcctgg ccgtgctgcg ccatcggata gccgtcgaaa
      241 gtggtcgcca ccgaccatgc ctcacccgga atgttgaaca ggatcgaggt gatggcgccg
      301 ccgaacagag cgccccaata gatgcaggac agcatgacga tggccgaggt cggatccatc
      361 gtgaaggtga gcggcagcag gatcgcgacg ccattcggtc cgcctaatcc aggcagtact
      421 ccgacgaaaa tgccgagcac gagcccgacc atcatcacgg ccagagtctt ccacgtcagc
      481 aaaacggtga agccgtgaag cagcaggccg aaggcttcca tcgcgactag cgttccgatt
      541 ccgaagtccg cacacccttt gcgcaaccct ctgatgcgaa cttcggaatc aaagggacgc
      601 tagctactct aggattctct agtgccgcct ttgaaaccga agttcgcagc tgcccttgca
      661 gcacggtctt gcgggaactt cgggttggac accagcctag tagccgaaag ccgcttcgag
      721 cgggcctttc ggcatgatga cgtcgaatgc gatgtcgaag gtgacgaaca tgatgattgc
      781 gaacaggaac gcggtgagta tcgacttcca cagcgcaatg cgtccaatga ttcgcatgaa
      841 ccccgcgatc aggagaaaac ttgcgacgta gagtccgagc cactgcgtga gcaggcaaaa
      901 cagcagcgtg gggacgaaga cttgcatcac tcggcggagc tggtcgcgag tgaggaatgt
      961 ctgcgtggca cccgcgcccg tcaccagcga ggtgatgagg ccgtaaagac tggctgcggc
     1021 aaggatgagc ccgaggtaga acgggaagta gccggcctgc gggccgtcgg cagcccatgc
     1081 gatcccggtg cgccagttgt cgaagcaaag cagcgccgcc aacgctaaca acagcagcga
     1141 aacgacgata tcgacggtgc gggtgccgac gacggatggc gaattatccg acggcgcggt
     1201 cgggtcttcg acgacgattt cggtttcggg tgtggccatt ggtctttttt cagcatcccc
     1261 gcgcgccgcg gtcattcacc cgcgaaacgg atgatccggt cagccgccgc tcccagttgc
     1321 ggcgctgaag gatgctggat gcctcacttt cgcgggggat gatcgtgcgc gctagtttgc
     1381 gacaaatccc gcctcggtca tcagattttt attcagcgca tcgtcctcct ccaggaactt
     1441 tatcatgtcg ttgccggtga ggaagatcgg cttgagcgct tgtttctcca tgtagtcctt
     1501 gtattcagcg gtctgcgtca actttttgaa cagatcgaca tagaacgcgg tctgttccgg
     1561 cgtcactttg cctggaagaa acatggcgcg cagcatcagg tattggatgt cgaggtcctg
     1621 ctccttgcag gtgggaatgt cgttccacga catgtcgctg atgaccttgg ccttgtattc
     1681 gatccgctcc ttgtcgaata cgcatagcgg ccgaacctga ccggcgcgcc agacttcgag
     1741 attctccgag ggattgttga cgttggaggc ggtgtgattg ccgacgagtt gggtcgccgc
     1801 ctcgccgccc gacttatatg gcagataggc gaatttcgcg ccggtcttct gctccagaaa
     1861 gacggtgagg acgtggtcct cgcggcgcga gcccgtgccg cccatcttga gcggtggcgc
     1921 ggcgctctta gcagcgtcgg tgaactcttt cacggtcttg taaggcagtt gcgtattcac
     1981 ccacagcacg aactgatcga gggcgatgac ggaaaccggg gtgaggtcgc gccagttgaa
     2041 cggaatcttg gccgagagcg gcagcatgta gatgagcgag taggcgatga ggactttatt
     2101 gggatcgcct tcgttcgatt tcatgtagat cagcgcctcg gctcccgacg cgccgccctt
     2161 gagcgagacg accatcggct gtttcatcag attgttcttc tgaatcgcgg cctgcatcat
     2221 ccgcgccatc tggtcggagg ctccgccggc accggcggca acgacgatct ccaccggctt
     2281 attc
//
LOCUS       DATLJT010001724         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4554799,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001724 DATLJT010000000
VERSION     DATLJT010001724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4554799"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..363
                     /locus_tag="VM910_39195"
                     /pseudo
     CDS             1..363
                     /locus_tag="VM910_39195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327356.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            672..845
                     /locus_tag="VM910_39200"
     CDS             672..845
                     /locus_tag="VM910_39200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68558.1"
                     /translation="MPHQPGKLQPNLFEEEAPRIELAAIQKADLAMLVEALLREIATA
                     LASGEIGDDQDHI"
     gene            826..2883
                     /locus_tag="VM910_39205"
     CDS             826..2883
                     /locus_tag="VM910_39205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI68559.1"
                     /translation="MTKITSEHLARSAYVYIRQSTADQLVHNHESRRRQYGLVDRAWQ
                     LGWTTVEVIDDDLGRSGGGITRPGFERLLAAICEGRVGAVLAIEASRLARNGRDWHTL
                     IEFCGLVGTIIVDEDGIYDPRHSNDRLLLGMKGTMSELELSLFRQRSQEALKQKARRG
                     ALILGVAAGYVKVGRDRIEKNPDQRVQDALKLVFSKFAEFQSARQVHIWLRDEGIELP
                     VKSRNAEARGIIWRLPAYNIVHNILTNPIYAGACAFGRTTSRVSVENGRKRVRRGVHR
                     PMTEWDVLIKDHHEAYITWDEFERNQSVIANNATGKGSATVKGAVRRGELLLPGLLRC
                     GHCGRKLHVSYSGKLGRYNCYGARTNHGTARCISIGGLGIDAAVSTEVLRHLKPLGVD
                     AAVKAIEGQTSETSAAQRQLELSLQQARYEAAHARRQYDAVDPANRLVAGELERRWNE
                     ALQAVNKLEGEIAAIVARKPPPLGERERQLLIQLGADLELAWSHPAATAATRKRILRA
                     ALSEIVVRKEGSVIDLILHWQGGDHTALQLKLRLNAAGRQHWPVPEDTTSLVRELARL
                     MPDRQIARLLNRCGKPTGYGNGWTQERVRGFRNHHDIAVYRDGEWAERGEITLDAAAE
                     IIGVSKMTALRMIRRGDVKGRQVCKGAPWVIKVEDVAAFDVQKRSSGSVTPNPAQQTF
                     VFQ"
     gene            3042..>3297
                     /locus_tag="VM910_39210"
     CDS             3042..>3297
                     /locus_tag="VM910_39210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HVI68560.1"
                     /translation="MAPALEVADIFRRHGAAFRQAHAGHLGCVERRIMSAITTCRTAA
                     LGGHVEQCDDCGATRIAYNSCRNRHCPKCQGLARVQWLADR"
BASE COUNT          627 a         1016 c         1079 g          575 t
ORIGIN      
        1 tctatgccgc cggactgcgc gtgtcggaag tcgttctctt aaagatcgcc gacatcgaca
       61 gccagcggat ggtgatccgg gtagagcagg gcaagggcgg caaggaccgc tacgtcatgc
      121 tttctccgca gctcctgcgg attttacgca cctattggcg gctcgctcga ccgaagcgat
      181 ggctgttccc gggccgtgac gatgagcgtc cgctcgtacc caacgtcttg cacgccgcct
      241 gccgctcagc ctgcgcggcg gctggcttga gcaagcaggt gacggtgcac acgctctcgg
      301 cacacatttg cgacccatct cttggagagc ggggcggacg tgcgcatcat ccaggtcttg
      361 ctcgaaacgg cgacatactg cccacgaaca cccggtcgaa ggacggatct actatcggtt
      421 ccatccctgg tgcggcgaga gcgtgctgat tgccaggcgg ctcgaatacc gtggtgtcga
      481 gctggtggtg attccgcaac ccgacagttc gctcgcctgc ataccggcgt ggatgacgca
      541 cgaagctgcg gctcaatacg cgctcagtga ggcgccccgt ttttcgctcg atgttctgcg
      601 gtccctgcgc accgagatcg atgcgctcct aggctttctc cagtccgaat caaagacgga
      661 ggaagccgat aatgccgcac cagcccggaa agctccaacc gaacctgttc gaggaggagg
      721 cgccacgcat cgagctggcg gccatacaga aggccgatct ggcgatgctg gtggaagccc
      781 tgctgcgcga gatcgcaaca gcgcttgcaa gcggggagat tggcgatgac caagatcaca
      841 tctgagcacc ttgcacgcag cgcgtacgtc tacatccgtc agtcgaccgc tgatcagctc
      901 gtgcacaatc acgagagcag gcgccggcag tacgggctgg tcgatcgcgc gtggcagctc
      961 ggctggacca ctgtcgaggt catcgacgat gatctgggcc gctcgggcgg cggcatcaca
     1021 cggccgggct tcgagcgcct gctggcggcg atctgcgagg ggcgcgtcgg cgccgtgctc
     1081 gcgatcgagg cgtctcggct cgcgcgcaac ggccgcgatt ggcatacgct gatcgagttc
     1141 tgtggattgg tcggtaccat catcgtggac gaggacggaa tctatgatcc tcgccattcc
     1201 aatgatcggc tattgttggg catgaaagga acgatgagcg agctggagct gtcgttgttc
     1261 cgccagcgct cacaggaggc gctgaagcag aaagcgcgcc gcggcgcact gatccttggc
     1321 gttgcagcag gctatgtgaa agtcgggcgt gaccggatcg agaagaatcc agaccagcgg
     1381 gttcaagatg cactgaagct cgtcttctcc aagttcgccg agttccaaag tgctcgtcag
     1441 gtccatattt ggctgcgcga tgaaggcatc gaattgcccg tcaagtcgcg caacgccgag
     1501 gcgcgcggta tcatttggag attgccggct tacaacatcg tgcacaacat tctgaccaac
     1561 ccgatctatg ccggcgcctg tgccttcggg cgtacgacga gcagggtgag tgtcgagaat
     1621 ggccgcaagc gtgtgcggcg cggtgtgcat cgtccaatga ccgaatggga tgttctgatc
     1681 aaggatcatc acgaagccta catcacctgg gacgagttcg agaggaacca gagcgtgatt
     1741 gccaacaacg cgaccggcaa aggcagtgcc acggtcaagg gagcggtgcg gcgcggcgag
     1801 ctgctgctgc ccgggcttct gcgctgtggt cattgcggcc gtaagctgca tgtgtcctac
     1861 agcggcaagc tcggccgcta taactgctat ggcgcccgca cgaaccatgg tacggcgcga
     1921 tgcatctcga tcggcgggct gggcatcgac gctgccgtca gcacagaggt gctgcgccac
     1981 ttgaagccgc tcggcgtcga cgctgcggtc aaggcgatcg agggccagac cagcgagaca
     2041 tcagccgcac aaaggcagct cgagctatct cttcaacaag cgcgatacga ggccgcccac
     2101 gcccgtcggc aatatgacgc cgtcgatccg gccaatcgcc tggtggccgg cgagttggag
     2161 cgccgctgga acgaggcatt gcaggccgtg aacaagttag aaggcgagat tgctgccatc
     2221 gtggcgcgca aacccccgcc gctgggcgag cgggagcggc agctgttgat tcagctcggc
     2281 gctgatctcg agctcgcctg gtcgcatccg gcagccacag ctgcgacacg caagaggatc
     2341 ctgcgcgccg cgctcagcga gatcgtcgtg cgcaaggaag gctcggtcat cgacctgatc
     2401 ctgcactggc agggcggaga ccacactgcg ctgcagctca agctgagatt gaatgcggct
     2461 ggccggcagc attggccggt gcctgaggat acgacatccc tcgttcgcga gctggcccgg
     2521 ctgatgccgg accggcagat tgcacgcctc ctcaatcgct gcggcaagcc gacgggctac
     2581 ggcaatggct ggacccaaga gcgtgttcgc ggcttccgta accaccacga catcgcggtg
     2641 taccgcgacg gcgaatgggc cgagcgcggc gagatcacac tcgacgccgc ggcggagatc
     2701 atcggcgtca gcaagatgac ggccctgcgt atgatccgtc gcggcgacgt caagggacgg
     2761 caggtctgca agggggcgcc ctgggtcatc aaggtcgagg acgtggccgc ctttgatgtc
     2821 cagaagcgct cttcaggttc ggtaacacca aatcccgcgc agcagacctt tgtgtttcaa
     2881 tgagatagga ggatgcgcat tatggcgcat gactgctcgg ccacgccagc ctcgcaagca
     2941 cggcgcgcta tacccgggtc gccaccaaaa cgatcagcaa cacgccgagc ccgctcgatc
     3001 ggctccgctt ggaggtcgcc cagcccggct gagcgggcgt catggctccg gctctggagg
     3061 tggcggatat cttccgccgc catggcgcag cgtttcgaca ggcgcacgcc ggtcatctcg
     3121 ggtgcgtcga gcggcgcatc atgagcgcca tcacgacctg ccgaacggcc gcgctcggcg
     3181 gccatgtcga acaatgcgat gattgtggcg caacgcgcat cgcttacaac tcctgccgca
     3241 accgtcactg tcccaaatgc caggggctgg cccgggtgca gtggctcgcg gatcggc
//
LOCUS       DATLJT010001725         7840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4097705,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001725 DATLJT010000000
VERSION     DATLJT010001725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4097705"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..533
                     /locus_tag="VM910_39215"
     CDS             411..533
                     /locus_tag="VM910_39215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68561.1"
                     /translation="MSTAAANDLREMVRALTLFPWFNTAEDEKRREAAGWALAH"
     gene            634..1632
                     /locus_tag="VM910_39220"
     CDS             634..1632
                     /locus_tag="VM910_39220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68562.1"
                     /translation="MQFDQLRRREFITLLGGAAAAWPLAARAQQPAMPVIGFLNAASA
                     DRYRPMVAAFRQGLQQSGYVEGQNVLIEYRWAEGQNNRLPALAADLVGRKVAVIAATT
                     SSGAVAAKAATTTIPVVFEVGADPVELGLVPSLDRPGRNVTGVSNFSGLLAAKVFEFL
                     HELMPNSRLIAVLVNPTSPNLARSTTKAMQAAGRALGQQIIVLNASTKDEIDAAFVAL
                     VQLRAGALAVGGDAFLNSRREQLVALAARHGVPAAYPWPEYVTAGGLMSYGPSLVDAY
                     RQTGVYVGRVLKGAKPADLPVMQPTKFKLVINLKTAKALGLEIPSSILARADEVIE"
     gene            1629..1763
                     /locus_tag="VM910_39225"
                     /pseudo
     CDS             1629..1763
                     /locus_tag="VM910_39225"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1801..1956
                     /locus_tag="VM910_39230"
     CDS             1801..1956
                     /locus_tag="VM910_39230"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI68563.1"
                     /translation="MSCSSISKGARPHDPSAAAASGISLKDAVVGLPLPRHPGAERYY
                     REMGILR"
     gene            complement(2018..2989)
                     /locus_tag="VM910_39235"
     CDS             complement(2018..2989)
                     /locus_tag="VM910_39235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI68564.1"
                     /translation="MTSTFRAACALLTTVVFLGIAVCGDARAAETIIIGTASPGGPYL
                     AYGRGLARILSRELGQEVTAESTQGPAQNITLLEKKQMMLAFTTMGVALQAWNGADWT
                     KGERYRSMRVIFPMYDTAFQFVALKRLGLRWLEDFAGLRIGVGPRAGTGGTYVPEILN
                     ALGIKASVRFGAMESMGAQLTAGDLDGVVFATGFPVPVLTTLIAAHPVDFVRPSPDQI
                     AIIRQKLPEISQSLVPAQTYPTQERDYPTIGLYNFAVAHKDLPNDLVYKIVKAVFDNH
                     DELVKAQSSANETVPANISRDAVLPLHPGAERYYREVGIPIPVEQTH"
     gene            complement(3093..3662)
                     /locus_tag="VM910_39240"
     CDS             complement(3093..3662)
                     /locus_tag="VM910_39240"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI68565.1"
                     /translation="MGQTAAPTIARRRQALTNVIGALCLAAALVVSVPVWSGSARAQA
                     TNWPRSITIGTASPGGVYDAYGEGLARMLTRTLQIEATAQVTQGPAQNIVLMEKKEAM
                     RGFVIMGVALQGWNRTHTAAQMAAGKLDGVAIASGFPIPALAESQSSAKETIPANIDR
                     NTMLSLHPGALRHYREIGIPVPPGATVGN"
     gene            complement(3729..4718)
                     /locus_tag="VM910_39245"
     CDS             complement(3729..4718)
                     /locus_tag="VM910_39245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HVI68566.1"
                     /translation="MRQVGAWGLVSALLISVPPAVNVAMAQEVASPRSIALATASPGG
                     VYLVYGQALAPILSRALQIEVTAEPTQGAVQNVILLEKRQTMLAFINVGPSLQGWNGS
                     AWAKGTHYRSMRVMFPLYDTGFQFVVHKRVAAKSLDNFTGLRIGGGPRGGTSGAYVEQ
                     IFKILGIPALVRHDAWDNLAQQLAERELDGAAAAIGAPFPALAQLDARQTIDFVAPSP
                     EQVASIRKQLPEITPSTIAAGTYRSLTTDYPTIGLYNFGVAHKDLSKDLVYRIVKAVF
                     ENHAELVKAHPVAKETVPENIERNTLFPLHPGALRYYREKGIAVPPAALGATN"
     gene            4952..>7840
                     /locus_tag="VM910_39250"
     CDS             4952..>7840
                     /locus_tag="VM910_39250"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68567.1"
                     /translation="MFETSVADSRDRSGEQTAVSEERIGLGLKGRLQFAFGAITLLVV
                     IAMGVGLYAFFQVGKSLQRITEEALPPALAASELSSRAEYIVGVGPALLASNDADEVN
                     RLSASVSSEFANVTKLLDQLRRADLDKAELDAIGEVISSLGANLALLQTTIWEKVAAE
                     TRREALVEATFAAHREFDAIWEPRFADMRGKVLRLQRALVSPNESQQDRRAELNDLDQ
                     AILALLPLEQIRRDFTLTFEIALRASTTNDPRELTEFRNQAQRSIRSIDGLVSDIDPD
                     LSTELFGPIARLRANVRGETNIFGLRQTEIETTAKSKQLIAENTALSAHLHNAVEQLV
                     TSSREDIDTAALVANRAQQFNTNVLLSVSALAVISSFLIVWLYVGRSIVARLTQLSGA
                     MLSIAAGRREIPVPATGSDEVAAMGRAVEVFRQNAIELDHLLAERANAAIRLEKLVEE
                     RTSELQRRGAVLRVTFDNMEHGVLMFDRELKLAAWNRQVMELLELPQAFLESKPRYDD
                     YLHLLAERGEYASGDAEAEVRRLTADVARDYRWERTRPDGTVLEIRHSGLPEGGCIII
                     YTDITERKRYEEALTAARDQAEAMSLTKSAFLASMSHELRTPLNAIIGLTEMMVTNAA
                     RFGTEKALEPLRRVHRAGTHLLGLINQVLDLSKIEAGKLELSPETVNLAPLLEDVIGT
                     ARQLAEQNKNRLVVESPDNLGSLTVDPMRLRQILLNLLSNACKFTQQGEVKLRVKKVV
                     DGRNLIEIAVADTGIGMTPEQQAKLFEEFTQADSSTARQYGGTGLGLAITRKLARMMG
                     GDVTVASEPGKGSVFTVRLPGSAEAQARSSTGSDGRRSPTAGCVLVIDDDATARELIA
                     DHLKAEGFSAVTAAGGMEGLKLAKELRPTAITLDVIMPDLDGWSVLTALRQDPELAEI
                     PVIMITIVDEHRRGIALGAAGYLTKPIDREQLHRLVSRFRAPVPPTRVL"
BASE COUNT         1516 a         2386 c         2427 g         1511 t
ORIGIN      
        1 gcaagccgag gatgcgcaag ggccgttcga ctgcgagcac ctgatttcgg ctgctaggtt
       61 gctgtgacac gcggcgggtc gcggtttgca gacaagcaag tcgtgttgga ttgcgcgtgg
      121 tgccgccgcg ccgcactcgt cggccaccgg tctattgcac tgcaagcaca tcgctcatct
      181 gacgaccccc acctagggag gacttttgga tccgactcgg tttgaccagc tttgaccatt
      241 tcggcggtgc tgacttgttg cgcatggttt gataatacgc catttcgctt gcgtacgcga
      301 cttgccagct gaggccaact cagcagctcc gctgtttctg ccgcctctgg tcgggccgct
      361 ctttttaggt gattaagccg tgaacgccca agaagcggcc gatatcttag gtgtcaaccg
      421 ccgccgccaa cgacttgcgt gagatggtgc gcgcgctcac gctgtttcct tggttcaaca
      481 ccgcagaaga cgaaaagcgc cgggaggccg caggatgggc gctggcgcat tagcccgagt
      541 atcaagcgga gtgttggaga cgccgggagg gatgccgctg actgatcgct cccaattcta
      601 tatcctacca tccggcttgg ctgcggggac tgaatgcaat ttgatcaatt gaggcggcgc
      661 gagttcatca cgctgctcgg cggagcggcg gcagcgtggc cgctcgcggc gcgcgcgcag
      721 cagcccgcga tgccggtgat cgggttcctc aacgccgcat cagctgacag gtaccggccc
      781 atggtggctg cgttccgtca gggcctccaa caatctggct atgtcgaagg tcagaacgtg
      841 ttgatcgaat accggtgggc ggagggtcaa aacaatcgat tgcctgccct ggcggccgat
      901 ctggttggtc gcaaggttgc cgtgattgcc gcgaccacca gttccggcgc agtggcggcc
      961 aaggcggcga ccaccacgat cccggttgtc ttcgaggtag gggccgaccc ggtcgagctt
     1021 ggacttgtcc ccagcctcga ccgaccaggc aggaacgtca cgggggtaag taatttcagc
     1081 ggcctgctgg cggcgaaggt gtttgaattc ctgcacgagc tgatgcctaa ctccaggttg
     1141 atcgccgtgc tggtgaatcc gaccagtccg aatcttgccc ggtccactac gaaagccatg
     1201 caggcggcgg ggcgcgccct cgggcagcaa atcattgttc tgaatgccag cactaaagac
     1261 gagatcgatg cagcatttgt ggctttggtc cagctgcggg caggcgcgct cgctgtgggt
     1321 ggcgatgcgt tcttgaacag ccgccgcgaa caactcgttg cgctggcggc acggcacggg
     1381 gtgccggcgg cttacccatg gcctgaatac gtcacggcgg gcggcctgat gagttacgga
     1441 ccgagcctcg ttgacgccta ccgtcagacc ggcgtctatg tcggccgggt tttgaaaggt
     1501 gccaagcccg ctgatcttcc cgtgatgcag ccaaccaaat tcaagctggt gatcaacctc
     1561 aagaccgcca aggcgctcgg cctcgaaatt ccgtcgtcga tacttgcccg cgccgacgag
     1621 gtgatcgagt gagggggcgc gacttgatca cgctaaaatg gcgcgagttc gccgccgctg
     1681 gcggcctatt aagctatgga agcagcgcta ccgacacgta ccgccaggcc ggcgtctaca
     1741 ccggccgaat tctcaagggc gagaagccga ccgacttgcc ggtggagcaa acgacgagag
     1801 ttgagctgtt catcaatctc aaaaggcgct cggcctcacg atccctctgc cgctgccgca
     1861 agtggaattt cgctcaagga tgccgtggtg ggattgccgc tgccgcggca tcctggcgcc
     1921 gagcgctatt atcgcgagat ggggatcctg cgctgagcgc ggcgttgatc tcggtgtaat
     1981 cgcagcacca ccaccgtctc accgatccag ggcgtggtca gtgcgtttgc tcaaccggga
     2041 ttgggatccc aacttcacga taatagcgct cggcgccggg atgcagcggg agaacagcgt
     2101 cacggctgat attcgccggg acggtctcgt tcgccgagga ttgggccttc accagctcgt
     2161 cgtgattgtc gaacacagct ttcacgatct tgtagacgag gtcgttgggc agatccttgt
     2221 gcgcgaccgc gaaattgtag agcccgatcg tcgggtagtc acgctcctga gtcggatagg
     2281 tttgggctgg aaccaacgac tgcgaaatct ccggcagttt ctgtctgatt atggcgattt
     2341 ggtcgggcga aggccgtacg aaatccaccg gatgcgctgc aatcaacgtc gtgagcactg
     2401 gaacagggaa gccggtggca aagacaacgc cgtccaaatc gcccgccgtc aactgggctc
     2461 ccatgctctc catagcgccg aaccggacgg aggccttgat gccgagagcg tttaatatct
     2521 ccggcacgta agtgcccccc gttccggctc gcgggccaac accaatacgt aagccagcaa
     2581 agtcctcgag ccaacggagt ccaagccgtt tcagcgcgac gaactgaaaa gccgtgtcgt
     2641 acatcgggaa tatcacccgc atggaacggt agcgctctcc cttggtccaa tcggccccgt
     2701 tccaggcctg cagggcgacg cccatcgtcg taaaggcaag catcatctgc tttttctcaa
     2761 gcagcgtgat gttctgcgcc gggccttgag ttgactctgc ggtgacctct tgtcccaact
     2821 cgcgggacag aatgcgagct aggcccctac cataagccag ataaggcccg cccggcgacg
     2881 ctgtcccgat gatgatcgtc tcggccgcac gggcatcccc acagactgca atgccgagaa
     2941 agacaacggt ggtaagaagc gcacatgcgg cacggaatgt agaggtcatc ttcttgcaac
     3001 cttttcggtg acccttatgg agcttatcgg tgaacgggct agctggtccg gcgccagcgt
     3061 cgtcatcact tgtcggtttc agtcttaagg tgtcagttac ccacagtcgc gcccggcgga
     3121 acggggatgc cgatctcgcg atagtggcgg agtgctcctg gatgaagcga gagcattgta
     3181 ttacgatcta tgtttgccgg aattgtctct ttcgccgacg attggctttc cgcaagcgcc
     3241 gggatcggga acccgctagc gatggccact ccgtcgagtt tgccagcagc catctgcgcg
     3301 gccgtgtgtg tcctgttcca gccctgtaac gcgaccccca tgatgacgaa gccgcgcatg
     3361 gcttctttct tttccatgag cacgatgttc tgggccggcc cctgggtcac ttgggcggtt
     3421 gcctcgattt gcaacgtgcg ggtcaacatc cgcgccagac cctcgccata ggcgtcgtag
     3481 accccgcccg gcgatgcggt cccaatggtg atggaacggg gccaattcgt cgcttgggcc
     3541 cgagccgacc cgctccaaac cggtactgaa actacgagcg ccgcggccaa gcacaacgca
     3601 ccgataacgt ttgtgagggc ctggcgccgg cgggcgatcg ttggggcagc agtctggccc
     3661 atcgcgcatc tcacctcgtt ggcgctgctg acggcagggg taacgcctcg ccgatcgagc
     3721 gcgatgcttc aattggtcgc cccgagtgcc gcggggggca ccgcaatccc cttttcgcgg
     3781 taataccgca gcgcccccgg atggagcggg aaaagcgtat tgcgctcgat attttcagga
     3841 acggtttcct tcgccaccgg atgcgccttc accagttccg cgtgattctc gaacaccgcc
     3901 ttgacgattc gatagaccag atctttggaa agatctttgt gggcgacacc aaagttatag
     3961 agcccgatgg tcggataatc cgtggttaag gatcgatacg tcccggccgc gattgtggag
     4021 ggggtgattt ctggcagctg ctttctgatg ctggcgacct gttccgggct gggcgcgacg
     4081 aagtctatgg tctgccgggc atcgagctgt gcaagagcgg gaaacggcgc cccaatggcg
     4141 gcggccgctc catcaagctc ccgctctgcc agttgctggg cgagattgtc ccaggcgtca
     4201 tgccgaacga gggcaggaat tccgagaatc ttgaagatct gctcgacgta agcgccgctg
     4261 gttccgccgc gcggcccccc accgatccgc aagcccgtga aattgtcgag cgacttggca
     4321 gcaacgcgct tgtgcaccac gaattgaaag cccgtatcgt acagcgggaa catgacgcgc
     4381 atcgagcgat agtgcgttcc cttcgcccaa gcactgccgt tccagccctg caagctcgga
     4441 ccgacattga tgaaggcgag catggtttgg cgtttttcga ggagaatgac attttgcacc
     4501 gcgccttgag tcggttcggc tgtcacctcg atctgcaaag cccgagagag aatgggggcc
     4561 aaggcttggc cgtagacgag atagacgccg cccggagatg ccgtggccag agcgatgctt
     4621 cggggggacg cgacctcttg cgccatcgcc acgttaacgg caggcggaac agagataaga
     4681 agcgccgaga ccagacccca ggcaccgacc tgtctcaccg gctcctcctc cctggagggt
     4741 tgatcaattg tattttatgc tccctggacg gttgatcaat tgtattttgt gaggagaagt
     4801 tgtaacagca taaaactgcg caggcaccta ccgaagtgac atcaggccta tcttggcggc
     4861 cacgggttcc cttcgaccct cgcgctgcat ccaccggagg aggcacgggg tctctgatgg
     4921 agggctcgca ggctgaagtc gcgagaccac gatgttcgaa acctcagtcg cagattctcg
     4981 cgacaggtcc ggcgagcaga ctgccgtctc agaggaaagg atcggtctcg gcctgaaagg
     5041 ccggcttcaa ttcgccttcg gtgcgatcac attgctcgtt gtgatcgcca tgggggtcgg
     5101 actttacgcc ttcttccagg ttggcaagtc gcttcagcga atcaccgagg aagcgctgcc
     5161 tccggcgctc gccgccagcg agctgtcgag cagggcagaa tatattgtcg gcgtcgggcc
     5221 ggcccttctg gcttcgaacg acgccgacga ggtcaacagg ctttccgctt ccgtctccag
     5281 cgagttcgca aacgtcacca agcttctcga ccaactgcgg cgcgctgacc tcgacaaggc
     5341 cgaactcgac gcaatcggcg aggtcatttc gagtctcggc gcgaatctcg cgctcctgca
     5401 aacaactatc tgggagaaag ttgccgcgga gaccaggcgc gaggcgttgg tcgaagcaac
     5461 tttcgcagcg catcgggaat tcgatgcgat ctgggagccg cgctttgccg acatgagagg
     5521 aaaggtcttg cggctgcagc gcgccctggt ttcaccgaac gagtcgcaac aggatcgtcg
     5581 ggcagagttg aacgatctcg atcaggcgat ccttgcgctg ttgccactgg agcagataag
     5641 gcgcgacttc accttgacgt tcgagatcgc ccttcgcgca tcgacgacga acgatccgcg
     5701 ggagctcacc gagttcagaa atcaggcgca acgttcgatc cgctcgatcg atggcctcgt
     5761 ttcagatatc gatcccgatc tgtcgaccga gctgttcggg ccgattgccc ggcttcgcgc
     5821 caacgtcaga ggtgagacga atatcttcgg cttgcgccag accgagatcg agacgacagc
     5881 caagagcaag cagctcatcg ctgagaatac tgcgctctcc gcccacctgc acaacgctgt
     5941 cgaacagctg gtcaccagct cgcgcgagga cattgacacc gcggcgctgg ttgccaaccg
     6001 ggcgcagcag ttcaacacga atgttcttct ctccgtctcc gcgctcgccg tgatcagctc
     6061 gtttctgatc gtttggctct atgtcggtcg cagcatcgtg gcgcggctga cgcaattgag
     6121 cggcgccatg ctctccatcg ccgcgggacg gcgcgaaatc cccgtccccg cgaccggatc
     6181 ggacgaagtt gccgcgatgg ggcgcgctgt cgaagttttc cggcaaaacg ccattgagct
     6241 cgaccatttg ctcgcggaac gcgccaacgc cgccatccgc ctggaaaagc tcgtggagga
     6301 gcgcacgtcc gaactgcagc ggcgcggagc agtcctacgt gtcaccttcg acaacatgga
     6361 acatggcgta ctgatgttcg accgcgagct gaagctggcc gcttggaacc ggcaggtcat
     6421 ggagttgctc gagctgcctc aggcattcct agagagcaaa ccccgatatg acgactacct
     6481 tcacttgcta gccgagcgcg gcgagtatgc gtcgggcgat gccgaagccg aagtgcgacg
     6541 gctcacggcc gacgtcgcgc gggattacag atgggagcga acccgtcctg acgggaccgt
     6601 gctggagata cggcacagtg gtttgccgga aggcggatgc attatcattt acacggatat
     6661 caccgagcgc aagcgttacg aggaggcgct caccgctgcg cgcgaccagg ccgaggcgat
     6721 gagcctcacc aagtcggcct ttctcgccag catgagccac gagctgcgca cgccgctcaa
     6781 cgccattatc gggctgacgg agatgatggt cacgaacgct gcgcgcttcg gcacggaaaa
     6841 ggcgctggaa ccgctgcgcc gggtacaccg tgccggtacc catcttctcg gcctcatcaa
     6901 ccaggtcctc gacctctcca aaatagaggc cggcaagctc gagctcagcc cggaaacggt
     6961 gaatctggcg ccgctgcttg aggacgtcat cggcaccgcg cgacaactgg cggagcagaa
     7021 taaaaatcga ctcgtagtcg aaagcccgga caatcttggc agcttgaccg tcgaccccat
     7081 gcggctgcgg cagatcctgc tcaatctgtt gagcaatgcc tgcaaattca cccaacaggg
     7141 cgaggttaag ctgcgcgtca aaaaggtggt cgatggccgc aatttgatcg agatcgctgt
     7201 ggccgatacc ggcatcggta tgactcccga gcagcaggcc aagttgttcg aggaattcac
     7261 ccaggctgat tcctcgaccg cacggcagta cggcggcacc gggctcggcc tagccatcac
     7321 gcgcaagctc gcgcgcatga tgggcggcga cgtgacggtg gcaagcgagc cgggcaaggg
     7381 atcggtcttt accgtccgcc tacccggcag cgcggaggcg caggcgagga gctcgaccgg
     7441 ttcagacggc cgccgttcgc ccactgccgg ctgcgttctc gtcatcgatg acgacgccac
     7501 tgcacgcgag ctcattgcgg atcacctcaa ggccgaagga ttttccgccg tgactgcggc
     7561 gggcggaatg gaggggctca agcttgccaa ggaactgcgg ccaaccgcga ttacgctcga
     7621 cgtcattatg cccgacctcg acggctggtc ggtgttaacc gctttgcggc aggaccctga
     7681 gctcgccgag atccccgtca tcatgatcac gatcgtggat gagcaccgcc ggggcattgc
     7741 cctcggcgcg gccggctatc tcaccaagcc gatcgaccgc gaacagctgc atcggttggt
     7801 tagccggttt cgggcaccgg tgccgcccac gcgcgtcctg
//
LOCUS       DATLJT010001726         1730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1109233,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001726 DATLJT010000000
VERSION     DATLJT010001726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109233"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VM910_39255"
     CDS             <1..625
                     /locus_tag="VM910_39255"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68568.1"
                     /translation="EDSGIKQPKDLEGASIANPAGGATVDMFPVYAKAAGLDASKVKW
                     VVAGSDSLPGLLASNRVPCVGQFTVGEPLLRIQAAPAKLIRFAFADAGVSFYGNGLIA
                     TDSTIATKADIVRRFVAATIRGMKDAFANPEEAGRIMNKYHPAIDVAVAEGETKAVAE
                     LAQVKGRELGLIDPARIEETINVINSVFKLKAPVAVNDVYAPGFVSK"
     gene            665..799
                     /locus_tag="VM910_39260"
     CDS             665..799
                     /locus_tag="VM910_39260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68569.1"
                     /translation="MSVARIAVPDFVSNSYFPAIAAIELGFFAREGLDVAHELIFPNY
                     "
     gene            812..1597
                     /locus_tag="VM910_39265"
     CDS             812..1597
                     /locus_tag="VM910_39265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68570.1"
                     /translation="MRDGKIDFVAGPAHAVFRAFPDWEGAKLLAALAQGMYWLLVIRT
                     DLAATPGDVNAVKGRTIGAAPLVDQGLKRLLIDAGLDLDRDGVHIVDVPGASEPGVSF
                     GVAAAKALEDGKLDGFWANAMGAETAVRRGVGKVVLDVRRGLGPRAAFHHTMPVLATS
                     DHAIKRDPNTVAAGVRSVVKAQQALKRDPSLAARVGKALFPPAEAALIADVVARDLPY
                     YDPTISEAAVDGLNRFAQASGLARRSVPYNQVVALEFRHLWTA"
BASE COUNT          322 a          498 c          573 g          337 t
ORIGIN      
        1 ggaggactcc ggtattaagc agcccaagga tctcgaagga gcctcgatag ccaatcccgc
       61 ggggggcgca acggtcgaca tgttcccggt ctacgctaag gccgccggct tggatgcgag
      121 caaggtgaag tgggtcgtcg cgggcagtga ttcgctgccg ggattgctgg caagcaatcg
      181 cgtgccgtgt gtcggtcagt tcacggtcgg cgaaccgctt ctgcgcatac aagccgcgcc
      241 ggccaagctc atccgtttcg cgtttgccga tgcgggtgtg agcttctacg gcaacggcct
      301 cattgcgacc gacagcacca tcgcgacgaa ggctgatatt gtgcggcggt tcgtcgccgc
      361 tacgattcga ggcatgaagg atgcttttgc aaatccggag gaggcgggtc ggattatgaa
      421 taaatatcat cccgcgatcg atgttgccgt tgccgagggg gaaaccaagg cagtggccga
      481 attggcgcaa gtcaagggca gggaactcgg gctgatcgat cccgcccgca tcgaagaaac
      541 catcaacgtc atcaatagcg tattcaaact caaggcgccg gtggcggtga acgacgtcta
      601 tgcgccagga ttcgtatcga agtgacgtgg ctgccaggcc cgcatcgacg ccccaggagt
      661 ccttgtgagc gttgcacgaa tagcggtccc cgatttcgtc tccaactcgt actttccggc
      721 gatcgctgcg atcgaactcg gcttctttgc gcgcgaagga cttgatgtcg cgcatgagtt
      781 gatcttcccg aactactagg cctacgaggc gatgcgcgac ggcaagatcg attttgttgc
      841 aggtccggcg cacgctgtct ttcgcgcatt cccggattgg gagggagcca agctgctcgc
      901 cgcgctagcg cagggaatgt actggcttct tgtcatacga acggacctcg cggcaacgcc
      961 gggtgatgtt aacgccgtca agggccgcac gattggggcg gcgccgctgg tggaccaggg
     1021 gttgaaacgc ctcttgatcg acgcaggcct cgatctcgat cgcgacggcg tacacatcgt
     1081 cgacgttccc ggcgcgagcg agccgggcgt ctcgttcggg gtggccgccg cgaaagcgct
     1141 cgaggatggc aaacttgatg ggttttgggc gaacgccatg ggggccgaga ccgccgtgcg
     1201 ccgcggcgtt ggcaaagtag tccttgacgt gcggcgcgga ttgggtccac gcgcggcttt
     1261 ccaccatacg atgccagtct tggcgacgag cgaccatgcg atcaagcgcg atccgaacac
     1321 ggtcgcggcc ggtgtgcgtt ccgtggtgaa ggctcagcag gctttgaaac gcgatcccag
     1381 ccttgccgca cgggtcggca aggcgttgtt tccgccggca gaggccgcgc tgatcgcgga
     1441 cgtggttgcg cgcgatctgc cctactacga cccgaccatc tccgaagcgg cggtcgacgg
     1501 cctcaatcgc tttgcccagg caagcggttt ggcgcggaga tcagtgcctt acaatcaggt
     1561 cgtcgccctg gagttccgtc acctctggac tgcctaacct gaaaaaaatc gtgccagcgc
     1621 ccaatcggcc ggtatcggag aactcattca gaatgagcgc ttatgcaacg gtgagggcgg
     1681 tgattggtgc tcgtgccgtg atcgcacttg tgctgctgag ggagggcgag
//
LOCUS       DATLJT010001727         1514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4098053,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001727 DATLJT010000000
VERSION     DATLJT010001727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4098053"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..>1514)
                     /locus_tag="VM910_39270"
     CDS             complement(399..>1514)
                     /locus_tag="VM910_39270"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVI68571.1"
                     /translation="TVPSGGLNTSVVAVITDVVRDAKPIVGYGFASIGRFAQGGLIRE
                     RFAPRLLAASDADLTDQAGTNLDPFRAWKVMMANEKPGGHGERCVAVGALDMALWDAV
                     GKIARLPLYRLLMERTGATQRDGPCTVSVYAGGGYYYPTDDIACLSDEVRRFRDLGYG
                     RTKIKIGSAPLARDLKRIDAAARLLDGPGNVAVDAIYAYTAATSPEAADALAPRGLWW
                     FEDICDPLDFETVATVARRYAAPIAAGEALFSAAEANLLDRHGGLRRDRDILVFDPVH
                     CYGLPGYLQIIELLEKRGWPRGAFWPHGGHLFCLHVVAALGLGGAEVNPLCFQPFGGY
                     GDGTNVANGTAIPPELPGIGFETKPALRDFLATVIDV"
BASE COUNT          302 a          477 c          468 g          267 t
ORIGIN      
        1 gagatcgggc cccatgggcg cgactactat cggagcctcg attccaaacc gttcgcaaaa
       61 ccgcgttcgc agcatgtcac gatcgtcctc ctacgagagc aaaatggggg cgatctggct
      121 aacgatgcaa ttgcgaacac attggcagtg gtttggcgga ggccatctga tgttaaatga
      181 ccgctgtcgg cgcgcgactt agccggtcac cacgagattg gtgaaggtcg gctaactcaa
      241 cggataccgg ccgttcagcc taagcggcgg gaatgggtca aagtgcccca ttccagaccc
      301 tcgccgccgc tggctcaaac cgtcggtttg ggtcgacgag tggagggagg tattgtaagc
      361 cgtcgtcaac tctgtgccgt atggcagcct tacgctgatc aaacgtcgat aacagtggcc
      421 aaaaagtctc ttagcgccgg ctttgtttca aagccgatac cgggaagctc tggcgggatc
      481 gcggtgccat tcgcgacgtt cgttccgtcg ccgtagccgc cgaagggttg gaaacagagt
      541 gggttgacct cggcaccgcc gagcccaagc gctgctacga catgtaggca gaagagatgc
      601 ccaccgtgtg gccaaaaggc cccgcgcggc cagccgcgct tttcaagcag ctcgatgatc
      661 tgcagatatc ccggtaggcc gtagcaatgg acaggatcaa aaaccaggat atccctgtcg
      721 cggcgcaatc cgccgtgccg gtcaagcaga ttggcttcgg ccgcggagaa gagcgcttcg
      781 cccgcggcga ttggggccgc gtagcgcctc gccaccgtgg cgaccgtttc gaaatccagc
      841 ggatcgcata tgtcctcgaa ccaccatagc ccgcgagggg caagcgcatc ggcggcctca
      901 ggactggtcg cggcggtata ggcgtatatg gcatccactg cgacattccc gggaccatcg
      961 agcagccgtg ccgcggcgtc gatccgtttc aaatcccgcg ccaacggcgc gctgccgatc
     1021 ttgatcttgg tgcgcccgta cccaaggtcg cgaaagcggc gcacctcgtc cgataagcaa
     1081 gcgatgtcat cggtgggata ataatagccg ccgccggcgt agaccgagac cgtgcagggc
     1141 ccatcgcgct gggtggcgcc cgtgcgctcc atcagaagtc ggtaaagcgg cagccgcgcg
     1201 atcttcccga cggcatccca caacgccatg tcgagcgccc cgacggcgac gcaacgctcg
     1261 ccatggccgc cgggcttttc gttcgccatc atgaccttcc acgcgcggaa cggatcgagg
     1321 ttcgttcccg cttgatccgt tagatctgca tcgctggcag cgagcagtcg cggtgcgaac
     1381 cgctcgcgaa tgaggccgcc ttgcgcgaag cggccgatcg atgcaaagcc gtaaccgacg
     1441 atcggcttgg cgtcccgaac aacatcggtg atcactgcca ccacactcgt gttgagcccg
     1501 cccgacggca cggt
//
LOCUS       DATLJT010001728         3447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3409156,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001728 DATLJT010000000
VERSION     DATLJT010001728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3409156"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VM910_39275"
     CDS             complement(<1..623)
                     /locus_tag="VM910_39275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVI68572.1"
                     /translation="MSYVALAAAGASEVRAQATPPAEQGATQSDEPRSSPSTSAPDAT
                     PPSAPAQEAPSSSPPATPAQDATQPGASSSPASPAQQQTETPALPAVTVEAPRRRPAS
                     SPAVQTAQVRTTLPSPPQPTAPQPSTADPSAAIQAAWPASGTQDARTGTVGVYANSTA
                     AATKINTPLIDIPQSLSVVTREFINDNSFQNLTDITRYVPGVDIHQG"
     gene            1547..2593
                     /locus_tag="VM910_39280"
     CDS             1547..2593
                     /locus_tag="VM910_39280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVI68573.1"
                     /translation="MQPDAMGVAMRLAAARLREAGIVLKPLLRSAGLSAIQMTNKDVR
                     IGVASQIRFLELAAKALNDPVLGFRLMHDVDLRHVGLLYYVAASSETLGNALERAQRY
                     SSIVNAGVVLKCFGARNFTITLRYAGVARHSDRQQMECVVTAIIRFCRNSTERRLNPI
                     AVHFVHRRLPQSSEIEKFFGCRIEFAANTDRIIFDKEAKQLPLVAADPYLNETLLRYC
                     EQALAYRRSREGPLQIAIENAIAPLLPHGKAQFGAIAQTLGVSSRTLARRLAAEGLSF
                     GEILSQLRSDLATHYLSDPNLPISEIAWLVGYQGVSAFSHGYKRWTGMSPKKMRGKLL
                     ASISGASARVRAGD"
     gene            2899..>3447
                     /locus_tag="VM910_39285"
     CDS             2899..>3447
                     /locus_tag="VM910_39285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVI68574.1"
                     /translation="MTESSPTEQTNALLAAVVASSMDAVFSIDTSARIQTWNSAAEQL
                     YGYTAAEAIGQPLGLLAPDPTNYAPGTRFQRVLQGEQIYFETSRRRKDGSLVEVGISS
                     GPIRDSSGHVVGVSVVHRDISERKRSERDLRQMHQWLEMAQEAGDVAPWAYDPKTGEV
                     RWTKQLYRQLGYGSDEITPSLSA"
BASE COUNT          644 a         1008 c         1074 g          721 t
ORIGIN      
        1 tcgccctggt ggatgtcgac gcccggaacg tagcgcgtga tgtcggtgag gttctgaaag
       61 ctgttgtcgt tgatgaactc ccgggtcacg acgctgagcg actggggaat gtcgatgagc
      121 ggcgtattga tcttggtcgc cgccgccgtg ctgttggcat agacgcccac cgtccccgtg
      181 cgcgcatctt gcgtgccgct cgccggccag gccgcctgta tcgccgcgct cggatccgcc
      241 gtgcttggct gcggcgcggt tggctgcggc gggctcggca gcgtcgtgcg cacctgcgcc
      301 gtctgcaccg ccggcgaact ggccgggcgc cgccgcggcg cttcgacggt gactgcaggc
      361 aacgcgggcg tctccgtttg ctgttgcgcc gggctcgccg gcgatgatga cgcgccgggc
      421 tgcgtggcgt cctgcgccgg cgtcgccggt ggcgacgagg aaggagcttc ctgcgccggc
      481 gcgctcggcg gcgtggcgtc tggcgccgac gtgctcggcg acgatcgcgg ctcgtccgat
      541 tgcgtggcgc cttgctcggc cggcggcgtt gcctgcgctc gcacctcgct cgcgcccgcc
      601 gcggcgagcg caacatagct caccgaaagg agcgccagct tcctgaccgc cccgatgcaa
      661 tgatgcgctc gattcttcct gcacatagcc cgcccccgaa gaaagtgtcg caaagtcgct
      721 gcgacagcgt ccctaacagg agtcggccgg ggaatcttcc gctatctttt tgaatgaatt
      781 tagtttttcc ccacgttggg tttattccaa ttcaaatgtc ggaatgaacg tcttaatgct
      841 gcggacgttt tggattcgac cagccggcag tcgtgtcaaa cggtgcgaaa gagatttcgg
      901 caacgcgttg caatcggatc acagatatgg cggacgcaga cacggatgcg tggtcgcgat
      961 ggcaagcgac ggctgattgc tcggccgccg cggcgcacgc aatgccggca gacagcactt
     1021 cgattgccct tgtcgagagc cgggtttttc tcttgagtgg ccggatctca aattacgagt
     1081 ggcctggtcc ggccaggacc gaaaccggcc aggtggcggc aagagctatc tggcggaatc
     1141 tcgcagtgct tgaatcttga gcaagagatc caccacaatg cgttgaagcc gtgcattctc
     1201 ggcttcgagg cgctccggta gtctctgatt gctgcgttgg gagacttgcc tatccgaacc
     1261 gctttttggt tcccgagcac gctctcgggc cgcttgcagg ctcagcaaat tttttgtcct
     1321 gcttgatcga gacatatcgg cctgggtcta acgcaaacgc cggtaacggc gaccggctcg
     1381 ctggacatgc aacacttcgg acaattctat taagttgcgg ggaaaggtgt tttgttattt
     1441 gctgccactg atttgctctt gcatgccgtg ctcgggagtc gttccgatgc gcagagccag
     1501 tcccaacctc tcgatgagaa acggagcaaa gtccgggcat cgggacttgc agccggacgc
     1561 gatgggcgtg gcgatgcgtt tggctgcagc acgcctgcgc gaggcgggca ttgtcctcaa
     1621 gccgctgctg agaagcgccg gtctgtctgc cattcagatg accaacaaag acgtgcgcat
     1681 cggcgttgcg agccagatca ggtttctgga gcttgccgcc aaagcattga acgatcctgt
     1741 gctcggcttc aggctgatgc acgacgtgga cctccggcat gtcgggctgc tctactacgt
     1801 tgccgcttcg tcggagacgc tcggcaacgc gctggagcgg gcccagcgct acagttcgat
     1861 cgtcaatgca ggcgtggttc tgaaatgttt tggagcccgc aatttcacga ttaccttgcg
     1921 ctatgctggc gtcgccagac attccgatcg gcaacagatg gaatgtgttg tgacagcgat
     1981 tatccgcttc tgcaggaatt caacggagcg ccgtctaaac ccgatcgccg tccatttcgt
     2041 tcatcgacgc ttgccccagt cctctgaaat agaaaaattc tttggatgcc ggatagaatt
     2101 tgctgcgaac acagaccgga tcatcttcga caaggaagca aagcagcttc ctctcgtcgc
     2161 cgcggatccg tatctcaacg agacgctgct ccgctattgc gagcaggcgc tggcatatcg
     2221 ccgctccaga gagggtccgc tgcagatcgc catcgaaaat gcgatcgctc cgctcctacc
     2281 gcatggtaaa gcgcagttcg gcgcgatcgc gcagacgctt ggggtgagca gccgaacgtt
     2341 ggctcggagg ctggctgcgg aaggcttgag cttcggtgaa attctcagtc aactccgctc
     2401 cgatctcgcc acgcattatc tgagtgaccc caaccttccg atctctgaga tagcatggct
     2461 cgttggttat cagggagtta gcgcattctc ccatggctac aagcggtgga ccggaatgag
     2521 cccgaagaag atgcgcggca aattgctggc gagcattagc ggagcttcgg ccagagtacg
     2581 agccggcgat taacctgccg ccgatgctgc tcgcccgcgc cgacgaggcg atcgaacagc
     2641 ggtgcttttg ctgcggcgcg caagggctcg aatgacgtgg tggacggcgc ccactcccgt
     2701 cgaccaatcg ccgtaaggtg atcgttgctg cgaatcacat cagggagcgg tccacatgca
     2761 aatgaccacg attgctttgg atattgcgca gtggaccagt ctacgggtgg gctggagatg
     2821 tgctatcctt tcggtcggaa ccacccgcgt gagcacacgt tagactggtt ggcgcaccga
     2881 ttgtaaggtg gccttgccat gaccgagagc agtccgactg aacagacgaa tgctcttcta
     2941 gccgcagttg tcgcttcgtc gatggacgct gttttcagca tcgatacgag cgcaagaatt
     3001 cagacatgga actcggccgc tgagcaactc tacggctaca cagcggccga agcgattggt
     3061 cagcctcttg gccttctcgc tcccgatccc acgaactatg cgccaggaac taggttccaa
     3121 cgcgtacttc aaggcgagca gatttatttc gaaacgtctc gtcgacgcaa ggacggaagt
     3181 ctggtcgagg ttgggatcag ctctgggcca attcgagact cgagtgggca cgtggttggc
     3241 gtgtccgtcg tccatcgcga catatccgaa cgcaagcgct ctgagcgcga cctgcgccag
     3301 atgcatcaat ggctcgagat ggcacaagag gcgggagatg tagccccgtg ggcgtacgac
     3361 cctaagaccg gcgaggtgcg ctggacaaaa caattgtacc gacagctggg ctatggttcg
     3421 gacgaaataa cgccgtctct gtcagca
//
LOCUS       DATLJT010001729         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4555750,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001729 DATLJT010000000
VERSION     DATLJT010001729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4555750"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2528)
                     /locus_tag="VM910_39290"
     CDS             complement(<1..2528)
                     /locus_tag="VM910_39290"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVI68575.1"
                     /translation="MIASFGIPAAYKDFQARNPIAPGRGTLAGRTALERRVVHIEDAA
                     TDPEYTRIEAVRLGNQRTMLGVPLVRDDALIGVITLARSRVEAFSEKEIALVAIFADQ
                     AVIAMENARLLSELRQRTTDLSKALDQQTATSEVLQGISSSPGELEPVFQAILAHATR
                     LCEASSGAMWLKEGDRFRNAAFHGALPAAYIEQWRSSRVDRTAPTGRVAQSRKPLQIA
                     DLREDQTYLDGYPLTVTAVDVAGIRTLAIVPMLKEDEFVGGISIYRKEVRPFTDKQIE
                     LVQNFANQAVIAIENTRLLNELRQSLQQQTATADVLKVISRSTFDLKTVLQTLVESVA
                     RLCEADQATITREIGGKFFRAEGYGFSSEFMSYIRDVPVEPERGTAHGRALLEGQMIH
                     IPDVLTDPDYTWAEAQRLGGFRTILGVPMLREGVPIGVLALTRSEVRPFTDKQVELVT
                     TFADQAAIAIENVRLFEEEATARAAAEAARDAAELARTEAAAARDAAEQARCEAEAAN
                     LAKSTFLATMSHEIRTPMNGVLGMIEVLERQRLTKDQRRIILTMRESAQALLHIIDDV
                     LDFSKIEAGRLELEATPFSLSGLVEGVLETFQPQVLAKGLTLDAEIDAGSQDALVGDP
                     TRVRQILFNLLSNAIKFTDHGGVRVRAGTLPLGGGSTLATLAVTDTGIGLGAEQLARL
                     FQPFVQADSSTTRQFGGTGLGLSIVQRLAQAMGGDVAVESAPGVGSTFTVTLTLHAAP
                     ADSPLKTLLGAVARPSARVAAQSGEGLRVLVVEDHPVNRDVLMLQLKLLGLAADTAEN
                     GVDALEAWARGRYAAVLADIHMPHMDGHELARRLRAAEADRGAVR"
BASE COUNT          456 a          833 c          803 g          483 t
ORIGIN      
        1 cggacggcgc cgcggtcggc ttcggcggcg cgcagccgcc gcgccaactc atggccatcc
       61 atgtgcggca tgtggatgtc ggcgagcacg gcggcgtagc ggccacgcgc ccatgcctcc
      121 agcgcatcga cgccgttctc cgcagtgtcg gcggcgagcc ccagcagctt gagctggagc
      181 atgagcacat cgcggttcac ggggtgatcc tcgaccacca ggacgcgcag gccctcgcct
      241 gactgcgcgg cgactctcgc ggaaggtctg gcaacagccc cgagcagcgt cttgagcggc
      301 gagtccgccg gcgccgcatg aagcgtcaat gtcacagtga aggtggaccc gacgccgggc
      361 gcgctctcga cagcaacatc accgcccatg gcctgcgcga gccgctggac gatggaaagg
      421 cctagcccgg tccctccgaa ttgccgtgtg gtcgagctat cggcctgtac gaagggttgg
      481 aacaggcgcg cgagttgctc tgcgcccagc ccaatgccgg tgtcggtcac ggcgagcgtt
      541 gcgagggtac tcccgccccc gagcggcaag gtgccggcgc gcaccctcac gccgccgtgg
      601 tcggtgaact tgatggcatt gcttaacagg ttgaacagaa tttgccgtac ccgtgtcgga
      661 tccccgacca aagcgtcctg cgagccggca tcgatctcgg catcgagcgt gagaccttta
      721 gcgagcacct gcggctggaa tgtctctagt accccttcga ccaggccgct cagcgagaac
      781 ggtgtagctt cgagttccag ccgtccggcc tcgatcttcg agaagtcgag cacgtcgtca
      841 atgatgtgca gcagtgcttg tgccgattct cgcatagtca ggatgatgcg ccgctgatcc
      901 tttgtgagcc gctggcgctc cagcacttcg atcatgccaa gcacgccgtt cataggcgtt
      961 cggatttcgt ggctcatggt ggcgagaaac gtcgacttcg ccaagttggc ggcctcggcc
     1021 tcgcaacgcg cctgctcggc tgcgtcgcgt gcggccgcgg cctcggtgcg cgccagttca
     1081 gccgcgtcgc gcgcggcctc ggcggctgcc ctggccgtgg cctcttcctc gaacagccgc
     1141 acgttctcga tcgcgattgc tgcctgatcg gcgaaggtgg tcaccagctc gacctgcttg
     1201 tcggtaaatg gccgcacctc ggagcgtgtc aatgctagga cgccgattgg gacgccctcg
     1261 cgcaacatcg gaaccccaag gatagtgcga aagccaccca acctctgcgc ctccgcccag
     1321 gtgtagtccg ggtcggttag gacatcgggg atgtgaatca tctggccctc gagcaatgct
     1381 cgcccgtgag ccgtcccacg ttctggctca acgggtacgt ctctgatgta actcatgaac
     1441 tcggaagaaa aaccataacc ctctgctcga aagaacttgc ccccgatctc tcgcgttatg
     1501 gtggcttgat cggcctcaca cagtcgtgcg accgattcga ccagcgtctg aagcaccgtc
     1561 ttgaggtcga acgtcgagcg gctgatgacc ttgagcacat cggcagtggc cgtctgctgc
     1621 tgcagcgatt ggcgcagctc gttgagcagg cgggtgttct cgatggcgat gacagcttgg
     1681 ttggcgaagt tctggaccag ctcgatctgc ttgtcggtga acggacggac ctccttacga
     1741 tagatggaga tgccgccgac gaactcatcc tccttgagca tgggcacgat ggccaatgtg
     1801 cggatgccag cgacatcgac tgcggtaaca gtcaacggat agccgtcgag atacgtctga
     1861 tcttctcgta ggtcagctat ctgaagtggc tttcgggatt gagcaacgcg gcccgtaggg
     1921 gctgtgcggt ccaccctgga gctccgccac tgctcgatat atgccgccgg cagcgcaccg
     1981 tggaatgcag cgttgcggaa gcgatctcct tctttgagcc acatagcgcc ggagctggcc
     2041 tcgcagagcc gcgtcgcgtg tgccagtatg gcctggaaca ccggttccag ctcacccggc
     2101 gagctggaga tgccctgtaa cacctcggag gtcgccgtct gctgatccag cgccttgctg
     2161 aggtctgttg tgcgctgccg caattcactc agcagccgag cattctccat cgcgatcacc
     2221 gcctggtcgg cgaagattgc gaccagcgcg atctcttttt cagagaaagc ttcgacacgc
     2281 gaccgggcaa gggtgatgac accgatcagc gcatcgtccc ggaccagcgg caccccgagc
     2341 atcgtacgct gattaccgag ccgcaccgct tcgatccggg tgtattcggg atcggtcgcc
     2401 gcatcttcga tatgcaccac gcgccgctcc aaagccgtcc gaccggccaa cgtgccgcgg
     2461 cccggcgcaa tcggattgcg cgcttggaag tccttatacg ctgccggtat cccgaacgag
     2521 gcaatcattc gacagagacc gccctgcagc cggaagataa aaccggaatc ggccg
//
LOCUS       DATLJT010001730         3331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_192114,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001730 DATLJT010000000
VERSION     DATLJT010001730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192114"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1261
                     /locus_tag="VM910_39295"
     CDS             152..1261
                     /locus_tag="VM910_39295"
                     /inference="COORDINATES: protein motif:HMM:NF021541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HVI68576.1"
                     /translation="MLPLRIVCPLAMLMILTSGASGQDVDDASFRNLAKQCQMRASVA
                     ASSRAIKIKDEAIAQHQAFNGTRVDRAGRIVFFGHSEAESDQEIDGNVSSRQIPWRQV
                     LEYWEKLNDRNIAEGAGGALQVWYYPGALSDDPPPVLHRKKMQLRRLLKFISRLDLSQ
                     MGPESDDLREALQQSVIRSSISDVPWSAAFVSSIMRAVPLDENTEFSYDASHVAYITS
                     AVEQSLRDLIGGESASFYRACDPDTTKPRIGDLFCYHRHIEGTPHQYAQKGPSLFKSL
                     FRDIAKGEEPISRSHCDIVVSVDSDSSKVTVIGGNVQNSVTEKVLNLNTKGVLSTAQG
                     STVCDSYNPDRPMSGGEPNCNPNRQKWFVLLQAAI"
     gene            complement(1279..>3331)
                     /locus_tag="VM910_39300"
     CDS             complement(1279..>3331)
                     /locus_tag="VM910_39300"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HVI68577.1"
                     /translation="RFLRPRSVAIVGISSRAGSAGQVILQSLNNFKGDIHLVGRSSET
                     IDGRPVLKSPDELPEGVDLAVFTLPAAGVRAAIEACARRDVGSALIFAAGFAEVGDQT
                     TQDAVTTTARSAGLAVVGPNCLGVTNNVDGIWLHMLYAREAQRGVVGGVAFVGQSGGL
                     LGHFQRAADGRGLPLTYVISTGNEAGLESTDFLEFLADDRSTRVIALYCEQIRRPQEF
                     LAACRRARAAGKPIVLMQAGRGAKARKAAQSHTGALIGDFATIRTQVEDTGAVVVHTM
                     DEMMDLVAILVRYPVPPTKGPSILTASGAYVALTNDFADEAGLELPALDAATLKKVRE
                     VLPAYGNYGNPLDVTAGFSPDALPIAVKALIDDPNVGMLFISFPISYAAVAVAFNKGM
                     ADSPKPKVMVALGDTWPLAPDIMQAINESPAVFSRSSDRMLRAITLYTRYGRSLARTR
                     VPPHPAPFAGMPRLGKRTQPEWLSKKILAAAGIRVPGGELARGLDHAVAIATRVGYPV
                     ALKAQAAALPHKSEAGGVMLNLADEAALRAAWETMQRNVERAAPGMTLDGCLVETMSP
                     KGLELMIGAKRDLAWGTVLLVGLGGIWVEALGDVELLPVDADEAQVVEALHKLRAAKL
                     LTGFRGARPVDVEAVAKAVLALGRLMQTTPDIVEIDVNPLMVHAEGQGATALDALIVA
                     E"
BASE COUNT          653 a         1026 c         1037 g          615 t
ORIGIN      
        1 ttcccaagct gtatgtcgag ggttcgattc ccttcgcccg ctccaactct ctgcgacgcc
       61 atcatttttc cccagcttcc agtaagacta cgcgccttat cgaatggcta cggtgcacgc
      121 gtagtcatct gcgaggccgg aggaagatcg tatgctgccg ctgagaatcg tttgtccatt
      181 ggcaatgttg atgatcctga cctctggggc ttccgggcag gacgtcgacg atgcgtcgtt
      241 caggaatctt gcgaagcaat gtcaaatgag agcgtcagtt gcggcatctt caagagcaat
      301 caagatcaaa gacgaggcga tcgcgcagca tcaggcattc aatggaaccc gtgtcgaccg
      361 ggcaggacga atagtctttt ttgggcactc ggaagcggag agcgaccagg aaatagacgg
      421 caacgtttct tcaagacaaa tcccatggcg tcaggtttta gaatattggg aaaaactcaa
      481 tgacaggaac atagcggaag gcgcgggggg cgcgcttcaa gtgtggtact atcccggtgc
      541 tcttagtgac gatccgccgc ccgttctcca tcggaaaaaa atgcagctac ggcgtctttt
      601 gaaattcata tcaagactcg atctgtctca aatggggccc gaatccgatg acctacgaga
      661 ggctctgcaa caaagcgtaa tccgcagtag tatcagcgac gttccgtggt cagccgcttt
      721 cgtatcttcc attatgcgcg ccgttcccct cgacgagaat accgagttct cctatgacgc
      781 cagtcacgtg gcctatatca cctccgccgt tgaacaatcc ctgagagatt tgatcggcgg
      841 ggagagcgcc agcttttacc gtgcttgcga tcccgacaca accaaacctc ggattggtga
      901 cctattttgc tatcatcgac acatcgaagg tacaccacac cagtacgctc aaaaggggcc
      961 ctccctgttc aagtcgctct tccgcgatat tgcgaaaggt gaggaaccca tcagccgcag
     1021 tcactgcgat atcgttgtca gcgtcgacag cgattccagt aaagtcactg tgataggagg
     1081 caacgtccag aattccgtga ccgaaaaggt acttaatcta aatacaaagg gcgttctttc
     1141 tacagctcag ggaagcactg tgtgcgacag ctacaaccct gatagaccca tgtcgggtgg
     1201 cgagcccaat tgcaatccga ataggcagaa gtggttcgtg ctcctacagg cagcgatctg
     1261 atagacggcg ggtctctttc actcggcgac gatcagcgca tcgagcgcgg tcgcgccttg
     1321 gccctcggcg tggaccatca acgggttgac gtcgatctcg acaatgtcgg gcgtggtctg
     1381 catcagccgg ccgagagcga ggacggcctt cgccaccgct tcgacgtcga cgggtcgcgc
     1441 gccgcggaat ccggtgagca gcttcgccgc gcgcaacttg tgcagcgcct cgacgacctg
     1501 cgcttcgtcg gcatcgaccg gcaaaagctc tacgtcgccc agcgcctcga cccagatgcc
     1561 gccgagcccg actagcagca ccgtccccca cgcgagatcg cgtttggccc caatcatcag
     1621 ttcgaggccc ttaggcgaca tggtctcgac caggcaaccg tcgagggtca tgccgggtgc
     1681 ggcgcgttcg acgtttcgtt gcatcgtctc ccaggccgca cgcagcgccg cctcatcggc
     1741 caagttgagc atgacgccgc cggcttcgct tttgtgcggc agcgccgcgg cttgtgcctt
     1801 taacgccacc ggatagccga cgcgcgtggc gatagcgacc gcgtggtcca gcccgcgggc
     1861 aagctcgcca ccgggcacgc ggatgccagc cgcggcgagg atcttcttgc tcaaccactc
     1921 cggctgggtg cgcttgccga gcctcggcat gcctgcaaac ggagcgggat gggggggcac
     1981 gcgggtgcgc gcgagcgagc gtccataccg ggtatagagc gtgatcgcgc gcagcatgcg
     2041 gtcggacgag cgcgagaaca ccgccggact ttcgttgatg gcctgcatga tgtcgggagc
     2101 gagcggccac gtatcgccga gcgccaccat gaccttgggc ttcggcgagt ccgccatgcc
     2161 cttattgaag gcgaccgcca ccgcggcgta gctgatggga aacgagatga acagcatccc
     2221 gacattggga tcgtcgatca gcgccttgac cgcgatgggc agcgcgtccg gcgaaaagcc
     2281 ggcagtgacg tcgagcgggt tgccgtagtt tccgtaagcc ggtagcacct cgcggacctt
     2341 cttcagcgtg gccgcatcga gcgcgggaag ctcgagaccg gcttcgtcgg cgaaatcgtt
     2401 ggtgagcgcc acgtatgcgc ccgaggcggt gaggatgctg gggcccttgg tgggaggcac
     2461 cggatagcgc accaggatag cgaccaaatc catcatctcg tccatggtat gaaccacgac
     2521 agcgccggtg tcctcgacct gggttcgtat ggtggcgaaa tcgccgatca gcgcgccggt
     2581 atgcgactgc gcggccttgc gcgctttggc gccgcggccc gcctgcatca acacgattgg
     2641 tttgccagcc gcgcgtgcgc gccgacaggc ggcgaggaat tcctgcggcc ggcgaatttg
     2701 ctcgcagtaa agcgcgatca cacgggtgga gcggtcgtca gccaggaatt cgagaaaatc
     2761 ggtgctttcg agccccgcct cattgccggt cgaaatcacg taggtgagcg gaaggccgcg
     2821 cccgtcggcg gcacgctgga agtggccgag caagccgccg ctctggccga caaaggcaac
     2881 gcctcccacg acgccgcgct gcgcctcgcg cgcgtagagc atgtgcagcc atatcccgtc
     2941 cacgttattg gtgacgccga ggcaattcgg ccccacgacg gcaagaccgg ccgaccgcgc
     3001 ggtggtggtg acggcgtcct gtgtcgtctg atccccgacc tcggcgaacc cggcggcgaa
     3061 tatcagcgcc gagccgacgt cgcggcgcgc gcaggcttcg atcgccgcgc gcacgccggc
     3121 ggccggcagc gtgaacacgg cgaggtccac gccctcgggc agctcgtcag ggctcttgag
     3181 cacgggcctg ccgtcgatcg tctcgctgct gcggccgacg agatggatat cgcccttgaa
     3241 gttgttgagc gattgcagaa tgacctggcc ggcgctgccc gcgcgcgaag agatcccgac
     3301 gatggcgacc gagcgcgggc gcagaaatct g
//
LOCUS       DATLJT010001731         4354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4557058,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001731 DATLJT010000000
VERSION     DATLJT010001731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4557058"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VM910_39305"
     CDS             <1..1764
                     /locus_tag="VM910_39305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HVI68578.1"
                     /translation="EIEGTFGVVTSTHWIATAVGMATLEKGGNAFDAGVATAFTLQVV
                     EPHLNGPGGDVPIIVHDVRRGRTEVICGQGPAPARATIGHYKSEGLEMVPGTGLLAAC
                     VPGTFESWMLLLRDYGTIRLRDVLEPAIAYARNGYPLVERASATIKTVEQLFRQHWPT
                     SAAVYLPNGAVPKPGTLFTNKQLSETYARILKEAESAGSDRVAQIERARQAWSKGFVA
                     EAIDKFCRTQDVMDVSGAPHKGVLSTDDMARWVPTVEAPLTYDYGPYTVCKAGVWSQG
                     PVMLQQLALLKGFQLDGLDPVSAEFIHLQVECAKLAYADREKFYGDPNFNEIPIQTLL
                     SDAYNDERRKLISEKASLDFIPGSVEGFGAVVKLRRAEGHREAVGAMGAGEPTVGRFG
                     EVRGDTVHFDIIDESGNMVSATPSGGWLQSSPVIPELGFCLGSRAQMFWLEEGHPASL
                     APGKRPRTTLSPTIALRDGEPYLAWGSPGGDQQDQWITQFFLRHVHAKLNLQEAIDAP
                     AWHSEHFPISFWPRTARPGVLVVENRVPKASREDLTRRGHTIEVGPDWSEGRLTAASR
                     LGRRRRAAANPRGMQGYAAGR"
     gene            2006..2716
                     /locus_tag="VM910_39310"
     CDS             2006..2716
                     /locus_tag="VM910_39310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HVI68579.1"
                     /translation="MTWSIIARDTATGQIGIAVATRFFAVGARVPHIAAGLGGIATQA
                     LVNPYYGIDGIRMLREGGSPQEIVSRLTSADDGRESRQLHILDAAGRIAAFTGKDCVD
                     WCGHLAGEGFSIAGNMLAGADVLEETAKTYLAGESLPFARRLIVAMRAGEAAGGDKRG
                     KQSAALLIHEREEWSALDLRVDDHDDPLAELERLEQVSRKHWVHFRKFLPTRQNPAGV
                     TDRATIDAGIEAAIAGKT"
     gene            2713..3702
                     /locus_tag="VM910_39315"
     CDS             2713..3702
                     /locus_tag="VM910_39315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68580.1"
                     /translation="MTENTLIDVRGLRVLFHGDDGRTTHAVDSVDLSVANGATLGLVG
                     ESGCGKSVTSLAIMGLLPKQSAEVSGSISFEGFDLLQTPDETLRDLRGNRLAMIFQEP
                     MTSLNPSFTIGDQIIETILRHRGGPRRAARERAIDLLRRVHIPSPERRIDDYPHKLSG
                     GMRQRVMIAMALACDPKLLIADEPTTALDVTLQAQILELMRELKAASGAAIILITHDL
                     GVVAEVCDDVAVMYAGEIVERAQIDELFTSPQHPYTVGLLGSIPRLERRTPHLATIEG
                     MVPNMTRLPTGCRFAARCPFASEICLKAPPPLTELKPGHASRCLKAPLERLVS"
     gene            3702..>4354
                     /locus_tag="VM910_39320"
     CDS             3702..>4354
                     /locus_tag="VM910_39320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVI68581.1"
                     /translation="MALLEVENLVKQFAADRNLFGRPTAFVKAVDGVSFRVDAGETLA
                     LVGESGCGKSTVSRLALRLIEPDAGRIRFEGRDLLALDANELRAFRRQAQIIFQDPYA
                     SLNPRMTVGQILSEPLALHNLVPQAGRRARVEEILTLVGLEPRFARRYPHEFSGGQRQ
                     RIAIARALAVEPKLIICDEPVSALDVSIRSQILNLLRDLQQRLGLSYIFVSHDLAVV"
BASE COUNT          756 a         1468 c         1381 g          749 t
ORIGIN      
        1 gaaatcgagg gcactttcgg ggtcgtgacc tccacccact ggatcgccac cgccgtcggg
       61 atggccacgc tggaaaaggg cggcaacgcc tttgacgccg gggttgccac cgccttcacg
      121 ctgcaggtgg tcgagccgca tctgaacggg cctggcggcg acgtgccgat catcgtgcac
      181 gatgtcaggc gcggccgcac cgaggtgatc tgcggccagg ggcctgcgcc cgcgcgcgcc
      241 accatcggcc attacaagag cgagggcctg gagatggtgc cgggcaccgg actgctcgcc
      301 gcctgcgtgc cgggaacttt cgaatcctgg atgctgctct tgcgcgatta cggcaccata
      361 cggctgcgcg atgtgctgga accggcgatc gcctacgccc gcaacggcta tccgcttgtc
      421 gagcgggcat ccgccaccat caagaccgtc gagcaattgt tccggcaaca ctggccgacg
      481 tcggcggccg tgtaccttcc caatggcgcg gtgcccaaac ccggcaccct cttcaccaac
      541 aagcagcttt cggagaccta cgcgcgcatc ctgaaggagg ccgagagcgc aggcagcgac
      601 cgcgtcgcac agatcgagcg tgcgcgccaa gcctggtcaa agggctttgt cgcggaagca
      661 atcgacaaat tctgtcgcac ccaggacgtg atggatgtct ccggcgcgcc gcacaagggc
      721 gtgctgtcga ccgacgacat ggcgcgctgg gtgccgaccg tagaggcgcc gctgacctac
      781 gactacggcc cctataccgt ttgcaaagcc ggcgtctgga gccaggggcc ggtcatgctg
      841 cagcagctcg cgctgctgaa gggttttcag ctcgatgggc tcgatcccgt cagcgccgag
      901 ttcatccatc ttcaggtcga atgcgccaag cttgcctatg ccgaccgcga gaaattctac
      961 ggcgatccca atttcaacga gatcccgatc cagacgctgc tgtcggatgc ctataacgac
     1021 gagcggcgca aattgatttc ggaaaaagcc tcgctcgatt tcattccggg ctcggtcgag
     1081 ggctttggcg cggtcgtcaa gctgcggcgc gcggaaggcc accgggaagc cgtcggtgcc
     1141 atgggcgcgg gcgagccgac ggtcggacgc tttggcgagg tgcgcggcga caccgtgcat
     1201 ttcgacatta tcgacgaaag cggcaacatg gtctcggcga cgccatcggg cggctggctg
     1261 cagtcatcgc ccgtgatccc cgaactcggc ttctgtctcg gcagccgcgc tcagatgttc
     1321 tggctggagg aaggtcatcc cgcctcgctc gcgcccggca aacggccgcg caccacgctc
     1381 tcgcccacca tcgcattgcg cgatggtgag ccctatctcg catggggatc gcccggcggc
     1441 gaccaacagg atcagtggat tacgcagttc ttcctgcgac atgttcacgc gaagctgaac
     1501 ctgcaggaag caatcgacgc tccagcctgg cattccgagc actttccgat ctcgttctgg
     1561 ccgcgcaccg cgcggcccgg tgtgcttgtg gtcgagaacc gcgtgccaaa ggcgtcacgc
     1621 gaggacctga cacgccgcgg ccacacaatc gaggtcggcc ccgattggtc ggaaggccgg
     1681 ctgacggcgg cctcgcgcct cggccgccgc cggcgtgcgg ccgccaaccc caggggtatg
     1741 cagggctacg ccgccggacg ctagagtgag acgagtcttg atcggatcca actctccgcg
     1801 cgctcgttca ccctcccccg gagggggagg gtcggcgagc atcgggcaac gatgcgagac
     1861 ggggtgggat ggtgttttat tgtgggcagc atcaccccac cccgatcgct catttcattc
     1921 gcgatcgacc ctccccctcc aggggcgggt aagcagttcc gctgccgcga cgacgcaact
     1981 tcgtttcacc atgaaataag ccgagatgac ctggtcgatc atcgcccgcg atacagccac
     2041 cggccagatc gggattgccg tcgcgaccag gttctttgcc gtcggtgcgc gcgtgccgca
     2101 tatcgcggcg ggcctcggcg gcatcgcaac gcaggcgctg gtcaatccct attacggcat
     2161 cgacggcatc aggatgttgc gcgaaggcgg gagtccgcag gagatcgtga gcaggctgac
     2221 ctcggccgat gacggtcgcg agagccgcca gctccacatc ctggatgcgg caggccgcat
     2281 cgccgctttc accggcaagg attgtgtcga ctggtgcggg catctcgccg gcgagggctt
     2341 ctcgatcgcc ggcaacatgc tggcgggcgc cgatgtgctg gaggaaaccg caaaaaccta
     2401 tctcgcaggc gagagcctgc cgttcgcgcg ccggctgatc gtggcgatgc gcgctggcga
     2461 agccgccggc ggcgacaagc gcggcaagca atcggcagcg ctcctgatcc acgagcgcga
     2521 agaatggtcc gcgcttgatc tgcgcgtcga cgaccacgac gatccgctcg cagagctcga
     2581 acggctggaa caggtgagcc gcaagcactg ggtgcatttt cgaaaattcc tgccgacacg
     2641 gcagaaccct gccggggtca ccgatcgcgc gaccatcgac gctggaattg aagccgccat
     2701 cgcgggcaag acatgaccga aaacaccctg atcgacgtgc ggggcctgcg cgtgctgttt
     2761 catggcgatg acgggcgcac cacccacgcc gtcgacagcg tcgatctctc cgtcgccaac
     2821 ggcgctacgc tcggcctggt cggcgaatcc ggctgcggca agagcgtcac ctcgcttgcg
     2881 atcatggggc tgttgccgaa gcaatcggcc gaggtctcag gctcgatcag cttcgagggt
     2941 ttcgatctcc tgcagacgcc ggacgaaacc ctgcgcgacc ttcgcggcaa ccggcttgcc
     3001 atgattttcc aggagccgat gacctcgctc aatccgagct tcacgatcgg cgaccagatc
     3061 atcgaaacca tattgcgtca tcgcggcgga cctcggcgcg cggcacgcga gcgcgcaatc
     3121 gacctgctgc gccgggtgca catcccctcg cccgaacggc gcatcgacga ttatccgcac
     3181 aagctttccg gcggcatgcg ccagcgcgtg atgatcgcca tggcgcttgc ctgcgatccg
     3241 aagcttctga tcgcggatga gccgaccact gcactcgacg tcaccctgca ggcgcagatc
     3301 ctcgaactga tgcgcgagct caaggcggcg agcggtgccg cgatcatcct gatcacccat
     3361 gatctcggcg tggtcgccga ggtctgcgat gacgttgccg tgatgtatgc cggcgagatc
     3421 gtcgagcgtg cgcaaatcga cgaattgttc acttcgcctc agcatcccta tacggtgggc
     3481 ctgctcggct cgatcccgcg gctcgagcgg cgcacgccgc atcttgcgac catcgagggc
     3541 atggtgccga acatgacacg cctgcccacc gggtgccgct ttgccgcacg ctgccctttt
     3601 gccagcgaga tctgcctgaa ggcgccgcca ccgctcacgg agctaaagcc cggccacgcc
     3661 tcgcgctgtc tgaaggcgcc gctggaaaga ctggtgtcct gatggcgctt ctggaggtcg
     3721 aaaacctcgt caagcaattc gccgccgatc gcaacctgtt cggccgtccg accgctttcg
     3781 tgaaagcggt cgacggcgtg agttttcgcg tcgacgccgg ggaaactctg gcgctggtcg
     3841 gcgagtccgg ttgcggcaaa tcgaccgtca gccgtctggc gttgcgcctc atcgagcccg
     3901 acgcaggccg catccgcttc gaggggcgcg acctgctcgc actcgatgcg aacgagctgc
     3961 gcgccttccg ccgccaggcc cagatcatct ttcaggaccc ctatgcctcg ctcaacccgc
     4021 gcatgaccgt gggtcagatc ctgagcgagc cgctcgcgct tcacaatctt gtgccacagg
     4081 ccgggcggcg cgcgcgggtc gaggagatcc tgacgctggt cgggctcgag ccgcgtttcg
     4141 cgcgccgcta cccccacgaa ttctccggcg gccagcgcca gcggatcgcg attgcccgcg
     4201 ccctcgcagt cgaaccgaaa ctgatcatct gcgacgagcc ggtgtccgca ctcgacgtgt
     4261 cgatccgttc ccaaatcctc aatctgttgc gcgatctgca gcaacggctc gggctttcct
     4321 acatcttcgt ctcgcacgac cttgccgtgg tcaa
//
LOCUS       DATLJT010001732         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1572093,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001732 DATLJT010000000
VERSION     DATLJT010001732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572093"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(501..1325)
                     /locus_tag="VM910_39325"
     CDS             complement(501..1325)
                     /locus_tag="VM910_39325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI68582.1"
                     /translation="MPSVAANGAEIPLVGLGTWDLRGRTCVRMVEQALRLGYRHIDTA
                     EMYDNEREVGEGLHASGIKRGDVFVTTKVWPSHFAPRDLERAARDSLTRLRLRQVDLL
                     LLHWPNPQIPLSKTLGALCKVKRDGVARHIGVSNFTVKLLEEAFRLSSEPLVCNQIEC
                     HPFLDQSKVIAACRKHGMAVVAYSPIARGGARNDKLLARIGAAYGKTAAQVALRFLVQ
                     HEIVVIPRTSKEQRLAENAGVFDFALSAAEMKEIAGLSHRDGRIVDWSYSGRAQWD"
     gene            complement(1340..2161)
                     /locus_tag="VM910_39330"
     CDS             complement(1340..2161)
                     /locus_tag="VM910_39330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI68583.1"
                     /translation="MDVIESHGARIPILGFGTMTLKEDVCVQLVEASLKLGYRHLDTA
                     QMYGNEREVGSGMRGSALKREDIFLTTKVWFTRLASGDFEHSVDESLERLALPWVDLL
                     MIHWPNAQVPLSESIASLCKMKQAGLTKHIGVANFNVAMIEEAVKLASEPIAVLQIET
                     HPYLDQSKVVAAARRHGMAVVGYCPLARGKVPADPVLQKIGHAHGKTPAPVALGFLEQ
                     QQIIPIPRTSKRERLAENLGSLDLRLSEAEMAEIAELKRPDSRIVSPPQAPKWDT"
     gene            complement(2270..2596)
                     /locus_tag="VM910_39335"
     CDS             complement(2270..2596)
                     /locus_tag="VM910_39335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVI68584.1"
                     /translation="MLRVRLKADGRLVEVLPDGSERPIASDPGAIASAAHNAARAEEA
                     RYARNVRGRTGLTQAAFAARIGVPVETVRNWEQGKRSPRGPARALLKVIEEAPDAAFA
                     VLAKAR"
BASE COUNT          511 a          896 c          885 g          541 t
ORIGIN      
        1 gcgcgcggcg tgagcgagaa agattacatg accggcaatc tgctcggccg cgaagtgaca
       61 gccgaagacg tcgcgcaggc gttcttgcgc caggcgctgg cgctgaaaac caccgccgac
      121 gtcacgaccg tcgacggcgg caatatcgcc gcggcgatgc gctgacccgt agcccgatcc
      181 gcggggcacc gcgtcgcgac agcggagcag cgtgggcaaa atcgttgacg cgcggcgccc
      241 acgcggtcgc acggaacaga tgattttgct cgccctactg gctatgccga gctgacggtc
      301 cgcatggttg ctgaatcaaa cgtcgtatgc gcgcttttct ttgccctctc cccgttgggg
      361 agagggttag ggtgaggggg tagagagttc gatccaaaaa tccgaacctc cctcacccgc
      421 tcgcttcgct cgcgacctct ccccactggg gtgaggtgac cggacttgtc ctgcactttg
      481 aggaaagaca aatgaacgtc tcaatcccac tgggcgcggc cggaatagga ccaatcgaca
      541 atgcggccgt cgcggtgcga aagcccggcg atttccttca tctccgccgc gctgagcgca
      601 aaatcgaaaa cgcctgcgtt ttccgcaagc cgctgttctt tgctggtgcg cgggatcacg
      661 acgatctcgt gttgtaccag aaatcgcaaa gctacctgcg ccgcagtctt gccatacgcg
      721 gcgccgatgc gcgccaacag cttgtcgttg cgcgcgccgc cgcgcgcgat cggactatag
      781 gcgacgaccg ccatgccgtg tttgcggcag gcggcaatca ccttggattg gtcgagaaat
      841 gggtggcatt cgatctgatt gcagaccagc ggttcggacg aaagccgaaa cgcttcctcg
      901 aggagcttca ccgtgaaatt ggacacgccg atatggcgcg cgaccccatc gcgcttgacc
      961 ttgcacagcg caccgagcgt cttgctgagc ggaatttgcg gattgggcca gtgaagcagc
     1021 aacaggtcga cttggcgcaa gcgcagccgg gtcagcgagt cgcgcgcggc gcgctcgagg
     1081 tcgcgcggcg cgaaatgcga cggccaaact ttggtcgtca caaagacgtc gccgcgcttg
     1141 atgccagagg cgtgcaggcc ctcgcccact tcgcgctcat tgtcgtacat ttccgcggta
     1201 tcgatgtggc ggtagccgag ccgcaacgcc tgttcgacca tgcggacgca ggtgcggccg
     1261 cgcagatccc aggtgccaag cccaacgagt ggaatctcgg cgccgttcgc agcgacactc
     1321 ggaattgccc cgagcgcgtt catgtgtccc atttcggcgc ctgcggcgga ctgacgatcc
     1381 gcgaatccgg gcgcttgagc tctgcgatct ccgccatttc cgcctcgctc agccgcaaat
     1441 cgagactacc gaggttttcg gcaagccgct cgcgcttgga cgtgcgcggg atcggaatga
     1501 tttgttgctg ttcgaggaaa cccagcgcca ccggcgccgg tgttttgccg tgagcgtgtc
     1561 cgatcttttg cagcaccggg tcggcgggca ctttgccgcg cgccagcgga caatagccga
     1621 cgaccgccat gccatgacgg cgcgccgccg cgacgacctt gctctggtcg agatagggat
     1681 gcgtttcgat ctgcaagacg gcgatcggtt cgcttgccag tttgaccgct tcttcaatca
     1741 tggcgacatt aaaattggcg acgccgatat gcttggttag tcccgcttgc ttcatcttgc
     1801 acagcgaagc aatcgattcc gaaagcggca cttgtgcgtt cggccaatgg atcatcaaca
     1861 agtcgaccca tggcagcgcc aagcgttcca ggctttcgtc cacggagtgc tcgaaatcgc
     1921 cagaggccaa gcgcgtgaac cagactttgg tggtgaggaa aatatcttcg cgcttaagcg
     1981 cagagccgcg catgcccgag ccgacctcgc gctcgttgcc gtacatttgc gcggtgtcga
     2041 gatgccggta gccgagcttg agcgatgctt ccaccaattg cacgcacacg tcctccttca
     2101 gcgtcatagt gccaaagccg aggatcggaa tgcgcgcgcc atgactttcg ataacgtcca
     2161 tgcgggtgat aacctccata aaatgccgcg gaatgccgcg ggcgggccgc agcatcgggg
     2221 attgccggcg gtaattccag cccttgccga gcgagggtgt tatgcggctt taacgcgctt
     2281 tggcgagcac ggcgaaggcg gcgtcgggcg cctcttcgat aactttgagg agcgcacggg
     2341 cgggaccgcg cggcgagcgc ttgccctgct cccagttgcg caccgtctcg actggcacgc
     2401 cgatgcgtgc cgcgaacgcg gcctgcgtga ggccggtccg gccccgcaca ttgcgcgcat
     2461 agcgggcttc ctcggcgcgt gcggcattgt gcgcggcgct ggcaattgcg cccggatcgc
     2521 tggcgatcgg acgttcgctg ccatccggca gcacctcgac caggcgtccg tccgctttca
     2581 accgcacgcg cagcattgcg cttgctctcc cccgcgttct ccaggagcgc catcataact
     2641 gattcgtcgt ggattcaatg agttagtacg ccactagcca ccttgatcta ggccgatgac
     2701 gcactcatac gatggcctag tcggccattg gccgctatca ggagtctcgc cctccccgcc
     2761 cgctgtaagg agttttggac gtgcagcttc tgcagatcga cgcccagcgg ctgtgggact
     2821 cgctgatgga gac
//
LOCUS       DATLJT010001733         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2951315,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001733 DATLJT010000000
VERSION     DATLJT010001733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951315"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VM910_39340"
     CDS             <1..439
                     /locus_tag="VM910_39340"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HVI68585.1"
                     /translation="RRFFMRGMSIGEVARKMGLRSSAIRYYEKLGLIPKAPRLSGRRR
                     YDERVLERLAMVRFAKHVGFSIAEIKVLLRGVDGRPPPERWRKLAAEKVAQVDAFVAQ
                     ARTIRKLLSETLDFQCPKLVERGRALPSAAARPAVRLKRVARS"
     gene            complement(464..1441)
                     /locus_tag="VM910_39345"
     CDS             complement(464..1441)
                     /locus_tag="VM910_39345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68586.1"
                     /translation="MKLSRRRFLHLVATAAALPAFSRCARAQAYPARPVRVLEGFGSG
                     SSADLVARLIGHWLSERLGQHFVIENRPGAGSNVATEAVVRSAPDGYTLLTCVTANAI
                     NATLYEKLNFNFIRDIAPVAGLLRMSLVVLVNPSLAAKTLPEFIDYAKANPGKVNIAV
                     PGNGSGPHVAGELFKMMTEINIVNVPYRGPAPAFTDLISGQVQVMFVALPAAIGYIKA
                     GELRALAVTGASRSSELPDVPAVAEFVQAYEASGWIGIGAPKSTPAEIIDKLNSNITA
                     GLADPQLKARIKGLGADPMPMTPTQFGALMGDETEKWAKVVKFAGIRAE"
     gene            complement(1460..2113)
                     /locus_tag="VM910_39350"
     CDS             complement(1460..2113)
                     /locus_tag="VM910_39350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HVI68587.1"
                     /translation="MPSLAQRAFSEWLGTAFLLAAVVGSGIMAQKLAGDDALALLCNT
                     LPTGAILAVLILVFGPLSGAHFNPAVSLGFALRGELPRSTAAIYVVAQVFGGLAGVWA
                     AHLMFELPVWQFSITQRTGPGQWLAEAVATFGLLLTIFGCVARRPESVPYAVGLYITA
                     AYWFTASTSFANPAVTIACSLSDTFTGIAPAGVGAFILSQFAGMVAAVILGRWLWRK"
     gene            complement(2113..2640)
                     /locus_tag="VM910_39355"
     CDS             complement(2113..2640)
                     /locus_tag="VM910_39355"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HVI68588.1"
                     /translation="MPDRPFNVLFLCTGNSARSIMAEAILNKLGAGKFRAFSAGSQPK
                     GQVNPHTRELLASLGYDTSGFRSKSWNEFAKPGAPALDFAFTVCDNAAGEICPVWPGQ
                     PMTAHWGIPDPAEAKGSPAEIALAFKDAYRMLHQRIGVFTALPIRSLDRLSLQRRLEE
                     IGRMQGATGKVTEPS"
     gene            complement(2649..>3072)
                     /locus_tag="VM910_39360"
     CDS             complement(2649..>3072)
                     /locus_tag="VM910_39360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68589.1"
                     /translation="SALFATQPSVVKPDYAKWMLDDPRVNFAISTRGKQPGLDHLGIQ
                     VESQDELQEVYAHLRAAGGNIVEQGQTSCCYAKSEKSWIDDPAGISWETFFTTGESTD
                     YGDGTGEREARVAHQKVCCEPQATSQSMPQTASACGTA"
BASE COUNT          611 a          955 c          963 g          543 t
ORIGIN      
        1 aaggagattt ttcatgcgcg ggatgagtat cggggaggtg gcgcgcaaga tggggctgcg
       61 ctcctcggct attcgttact acgaaaagct cgggctgatc ccgaaagcac cgcggttgag
      121 cggccgccga cgctacgacg agcgcgtgct tgagcggctg gcgatggtgc gatttgccaa
      181 gcacgtgggt ttcagcatcg ccgagattaa ggtgctgtta cgcggcgtcg acggccgccc
      241 gccgcccgag cggtggcgca agctcgccgc cgagaaagtc gctcaagtcg acgcattcgt
      301 tgcgcaggcc cgaacgatcc gaaaattgct gtccgagacg ctggacttcc agtgtccaaa
      361 attggtggaa cgcggtcgtg ctttgccgtc cgcagccgca cgtccagcgg tgcgcctgaa
      421 gcgtgtcgcc agatcgtaag ggagcccaac gagatttatc gcatcattcc gctctgatac
      481 cggcgaactt caccaccttg gcccatttct cggtttcgtc gcccataagc gccccaaact
      541 gggtgggcgt catcggcatt ggatcggcgc ccagaccttt gatccgagcc ttgagctgcg
      601 ggtcggcaag tccagcagtg atattgctgt tgagcttatc gatgatttcg gcgggggtgc
      661 tcttgggcgc accgatcccg atccaaccgc tcgcctcgta agcttggaca aattccgcga
      721 ccgctggcac gtccggtagc tcggacgagc gcgatgcacc ggtaaccgcc aatgcccgca
      781 actcgcccgc cttgatgtaa ccgatcgcag ccggcaaagc cacgaacatc acctgcactt
      841 ggccgctgat caggtcagtg aacgcgggcg ctgggccgcg atagggcaca ttaacgatgt
      901 taatttcagt catcatcttg aacagctcgc ccgcaacatg ggggccgctc ccgttacccg
      961 gcaccgcgat gttcaccttg ccgggattgg ccttggcgta gtcgatgaac tcgggaagag
     1021 tcttggctgc aagcgatggg ttgaccagca cgaccagaga catccggagc agtccggcga
     1081 ccggtgcgat gtcgcggatg aaattgaaat tcagcttctc gtagagcgtc gcattgatcg
     1141 cgttcgcagt aacgcaggta agaagcgtgt agccgtccgg ggctgaacgt acaacggcct
     1201 cggtggcgac attgctgcca gcgccgggcc ggttctcgat aacaaaatgc tgccccagcc
     1261 gctccgacag ccagtgaccg atcagacgcg cgacaaggtc ggctgagcta ccgctaccaa
     1321 atccttcaag cacccgcacc ggccgcgcgg gatacgcttg cgcccgcgcg cagcgagaga
     1381 atgccggcag cgcggcagca gtggctacca ggtgcagaaa tcgacggcgg gaaagtttca
     1441 tggctcccct ctccgtcatt catttgcgcc aaagccaacg accgaggatg accgctgcga
     1501 ccattcccgc aaactgcgag aggatgaagg cccctacccc agcgggcgca attccagtga
     1561 acgtgtcgga cagcgagcag gcaatggtga cggcaggatt ggcgaaagaa gtcgaagccg
     1621 tgaaccaata ggccgccgtg atgtagagac cgacggcgta aggaacggac tcaggcctcc
     1681 gtgcgacgca gccgaagata gtcaatagaa ggccgaaggt cgcgacagct tccgccagcc
     1741 attgtcctgg acctgttcgc tgcgtgatcg agaactgcca caccggcagt tcgaacatga
     1801 gatgcgccgc ccacacgccc gcgaggccgc cgaatacttg cgccacgacg taaatcgcgg
     1861 ccgtcgacct gggcaactcg ccgcgcaggg cgaatccaag acttaccgcc gggttgaagt
     1921 gcgcgcccga aagcgggccg aataccagga tcaagacggc gaggatcgcg ccggtcggga
     1981 gtgtattgca caggagcgcg agcgcgtcgt cgcccgcgag tttttgcgcc atgataccgg
     2041 agccgacgac ggccgcgagc agaaaggcgg tccccaacca ttcggaaaac gcgcgttgtg
     2101 cgagcgaggg catcagctcg gctcggtcac cttgccggtt gcgccctgca tacggccgat
     2161 ctcctcgagc cgccgttgca agctcaaccg atcgaggctg cggatcggca gcgccgtgaa
     2221 aacgccgatg cgctgatgca gcatccggta cgcatccttg aacgcaagcg cgatctcagc
     2281 cggcgagcct ttggcttcgg ctggatcggg aatgccccaa tgcgcggtca tcggctggcc
     2341 cggccacacc ggacagattt cgcccgccgc gttgtcgcag acggtgaagg caaaatcgag
     2401 cgcaggcgcg ccgggcttgg cgaactcatt ccacgacttc gagcgaaagc ccgacgtgtc
     2461 atagcccagg ctcgccaaga gctccctcgt gtgcgggttc acctggccct tcggctggct
     2521 gcccgcactg aaggcacgga atttaccggc gccgagcttg ttcaggatgg cctcggccat
     2581 gatcgaacgc gccgagttgc ccgtgcataa gaaaaggacg ttgaagggac ggtccggcat
     2641 gtccacgcct atgccgtgcc gcaagccgac gccgtttgag gcatggattg cgaggtagct
     2701 tgcggctcgc agcacacctt ttggtgagcc acgcgcgcct cacgctcgcc ggtgccatcg
     2761 ccgtaatcgg tgctctcgcc agtagtgaag aacgtttccc aggagatgcc ggccggatcg
     2821 tcgatccacg atttttccga cttcgcatag cagcaggaag tttggccctg ctcgacgata
     2881 ttgccgcccg ctgcgcgcag atgcgcatag acttcctgca attcgtcctg gctctcgact
     2941 tggatgccga ggtgatcgag gcccggctgc ttgccccgcg tcgagatcgc gaaattcacg
     3001 cgcgggtcgt ccaacatcca tttggcatag tccggcttga ccacggaagg ctgggtcgcg
     3061 aacagcgccg aa
//
LOCUS       DATLJT010001734         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2724556,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001734 DATLJT010000000
VERSION     DATLJT010001734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724556"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VM910_39365"
     CDS             <1..1263
                     /locus_tag="VM910_39365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVI68590.1"
                     /translation="RDPTASLEATEKFWKDWTSKCRAAGEWTDAVRRSMITLKALTYA
                     PTGGIVAAPTTSLPEKIGGERNWDYRFCWLRDATLTLLGAMHAGYYEEAEAWREWLLR
                     AVAGSPDQLQIMYGIGGERRLSEWVVDWLPGYEKSSPVRVGNAAHTQLQLDMFGEIMD
                     VHHQARRGGLTTNESGWDAQIKFLEYLARIWREPDQGIWEMRGPAQHFTYSKVMAWVA
                     FDRTIKSAENFGLEGPLEEWRRLRDEICDDVCTHAYNKKLGTFVQAYGSDQLDASLLM
                     VPCVGFLPVRDPRIAATIAAIERRLLRDGLVIRYCTEDVTDALPPGEGAFLACSFWLV
                     DVYILQERFEEAENLFRRLVGLCNEVGLLSEEYDPSSRRLIGNFPQAFSHMALVNSAY
                     NLTRVRKPLLQRAQDEHAPPAAVREKVP"
     gene            complement(1331..2236)
                     /locus_tag="VM910_39370"
     CDS             complement(1331..2236)
                     /locus_tag="VM910_39370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HVI68591.1"
                     /translation="MSIRPIKRIVQSKPTIEGAGVKLRRAFGFGDTQETDPFLLLDDF
                     RNDRPQDYLAGFPWHPHRGIETITYVLSGTVEHGDSLGNRGTLGAGDVQWMTAGRGIM
                     HQEMPQGDTQGRMHGFQLWANLPSSLKMRAPRYQDIKAGEIPEVVDDDGTRVRVVCGE
                     FWGKKGPVDGVAADPRYLDIAVSAGKRKKLKVETERHAFAYVFEGDGRFASASRPLGV
                     LTEKQVDGEELLVREPAGDRSLVLFDSGDEVEVQAGERGIRFLLVSGKPIEEPVAWYG
                     PIVMNTRAQLEQAVAELRNGTFIRG"
     gene            complement(2680..>3370)
                     /locus_tag="VM910_39375"
     CDS             complement(2680..>3370)
                     /locus_tag="VM910_39375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HVI68592.1"
                     /translation="GIVLAANLKALMAAAESTDGVIELAPQVGDFVAVDEPLFNLYGG
                     ARVLDETPLRNAIAFGSERTMEQDPTFAFRIVVDIALKALSPAINDPTTAVLAIDQLH
                     RMLRSVGRRDLRTDEILDASGRLRVISRTPNWDDFVNLAFSEIRACGANNLQIIRRLR
                     AMIENLKETLPSHRHDELQRQLNLLDSEAKRLLRYPEELALAGIADSQGLGGHSSKYT
                     LENKNNRIPGV"
BASE COUNT          628 a         1042 c         1060 g          640 t
ORIGIN      
        1 cgcgatccaa ccgcctcctt ggaggcaacc gaaaaattct ggaaggattg gacgagcaaa
       61 tgccgtgccg ccggcgaatg gaccgacgcc gtgcgtcgct ccatgatcac gctcaaggcg
      121 ctcacctatg cgccgaccgg cggcatcgtc gccgcgccga ccacgtcgct gccggaaaag
      181 atcggcggcg agcgcaattg ggactaccgg ttttgctggc tgcgcgatgc tacgctcacg
      241 ctccttggcg ccatgcacgc cgggtactat gaagaggcgg aggcttggcg cgaatggttg
      301 ttgcgcgcgg tggccggcag tcccgaccag ctgcagatca tgtacggcat tggcggcgag
      361 cggcggctga gcgaatgggt cgtcgactgg ctgccaggct acgagaaatc ctcgccagtg
      421 cgcgtcggca atgccgcgca cacccagctg cagctcgata tgttcgggga gattatggac
      481 gtgcaccatc aggcgcggcg cggcggattg accaccaacg aatcgggatg ggacgcgcaa
      541 atcaagtttc tcgaatatct ggcgcgaatc tggcgtgagc cggatcaggg catctgggaa
      601 atgcgcggcc cggcgcagca tttcacctat tccaaggtca tggcctgggt tgccttcgat
      661 cgcactatca agagcgctga aaatttcggc ctcgaagggc cgctcgagga gtggcgaaga
      721 ttgcgcgacg agatttgcga cgacgtgtgc acgcacgcct ataacaagaa gctcggcacg
      781 ttcgtgcagg cctatggttc cgaccagctc gacgccagcc tgctgatggt gccgtgcgtc
      841 ggcttcctgc cagtaaggga tccgcgcata gccgctacca tcgcggcgat cgaacgaaga
      901 ctcctccgtg acggcctggt catccgctat tgcaccgagg acgtaacaga cgcgctgccg
      961 cccggtgagg gcgccttcct tgcctgcagc ttctggctgg tcgatgtcta cattttgcag
     1021 gaacgcttcg aagaagccga aaacctgttc cgtcggctcg tcgggctttg caacgaagtc
     1081 ggtctactca gtgaggaata cgatccgagt tccagacgcc tgatcggcaa ctttccccag
     1141 gccttttcgc atatggctct ggtcaacagc gcctacaact tgacgcgcgt gcgcaagccc
     1201 ttgcttcagc gcgctcagga cgagcacgct ccacccgccg cggtgcggga gaaggtgcca
     1261 taggcggacg ccggcctcgc cgatcgccgt tgtgaagtcg gttgcggtcc cggattcgcc
     1321 ggtccgcatg ctaacccctg atgaacgtac cgttgcgcag ttcggcgacc gcctgctcga
     1381 gctgcgcgcg cgtattcatc acgatcggcc cgtaccaggc caccggctcc tcgatcggct
     1441 tgccggacac cagcaagaat cggatgccgc gctcgccggc ctgaacttcg acctcgtcgc
     1501 cggagtcgaa cagcaccagc gagcggtcgc ccgccggttc gcgcacaagc agctcctcgc
     1561 cgtcgacctg cttctcggtg agaacgccca agggccgcga cgcagacgca aaacggccgt
     1621 ctccctcgaa aacgtaagcg aaggcatggc gctcagtttc gactttcagc tttttacgct
     1681 tgccggcaga taccgcgatg tcgaggtagc gcgggtcggc ggcgacgccg tcgaccgggc
     1741 cctttttgcc ccaaaactcg ccgcacacga cgcgcacgcg cgtaccgtcg tcgtcgacca
     1801 cttcgggaat ctcgcccgct ttgatgtcct gatagcgcgg agcccgcatc ttcagtgacg
     1861 acggcaggtt ggcccagagc tgaaagccgt gcatacggcc ttgcgtatcg ccttgcggca
     1921 tctcctgatg catgatgccg cggcctgcgg tcatccattg cacgtcgccg gcgccgagcg
     1981 tgccgcgatt gccaaggctg tcgccatgct cgaccgtgcc ggagagcaca taggtgatgg
     2041 tctcgatgcc gcgatgcgga tgccagggga aaccggcgag ataatcctgc ggacggtcgt
     2101 tgcggaaatc gtcaagcagt aggaaaggat cggtttcttg agtatcgcca aaaccgaaag
     2161 cgcggcgcaa cttcacgccg gcgccttcga tcgtcggctt ggactgcacg atgcgtttta
     2221 tggggcgaat ggacatggcg atgacgctcg cgctgcgccg cgagccctcc cagattgtgg
     2281 ctagttgtca cgctagataa atgcggtagg cgcggccgca aggaggcaca ccgctgatac
     2341 cgcggtaccg ctcaataatg ccgagcgtcc cacgccgccg cgatcattgc gccagcgctt
     2401 tggtatgggc gtcttcgccg atgctcttat agtcgtaagt ggcgtagaac tcggtccggt
     2461 agccgccccc accccgtctg ctcgatcacg cgattaacgt cgcgcagccg ctcggcgggt
     2521 acgcgcaaga ggcgaggcgc cgtctagtgc gccgccggtt tgtgcggttg cttatgcgat
     2581 gaccgggtga gaatgcagcc gcacagcgcc aatatcgcaa cggctaccag cgaaagcgtg
     2641 cgagacttgt ttggcgagct aatccgggcc ccagggcggt cagacacctg gaattcgatt
     2701 gtttttattc tccagagtgt acttgctgga atgcccgccc agcccttggg agtcggcaat
     2761 tccggcgagc gccagttctt cgggataacg gagtagtctc ttggcttcgc tgtcgagcag
     2821 attgagttga cgttggagct cgtcgtgacg atggcttggc aaggtctctt tgaggttttc
     2881 gatcatcgcg cgcaaacggc gaatgatctg aagattgttt gccccgcagg ccctgatctc
     2941 gctgaatgcc agattgacga aatcgtccca attcggggtc cgggatatca cgcgcaaccg
     3001 tcccgaagca tccaagattt catcggtgcg cagatcgcgt cggccgaccg accgcagcat
     3061 gcggtgcagc tggtcgattg cgagcacggc ggtggtcgga tcgttgatcg caggggataa
     3121 cgccttgagc gcaatgtcga ccacgatccg gaacgcgaag gtcggatctt gctccatagt
     3181 ccgctcggag ccgaatgcaa tcgcgttgcg cagcggcgtt tcatcgagca cgcgcgcgcc
     3241 accgtagagg ttgaacagcg gctcatcgac ggcaacgaaa tcgccgactt ggggcgcgag
     3301 ttcgatcacg ccgtcggtgc tctccgcagc ggccatcagg gccttcagat tggccgcaag
     3361 aacgatgccc
//
LOCUS       DATLJT010001735         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2724600,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001735 DATLJT010000000
VERSION     DATLJT010001735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724600"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VM910_39380"
     CDS             complement(<1..600)
                     /locus_tag="VM910_39380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68593.1"
                     /translation="MRNVLVTGGSRGIGLAIAQRLATAGYNVIAVARRESSELRRAIS
                     ELGHGGLHFLACDLSQVDAIPDFVKAVRREFGAIYGLVNNAGGGSEGLLATMHNADIE
                     ALVRLNVLSPIVLSKYVVRHMMADGEGRIVNISSIIASTGYNGLSVYGASKAAATGFT
                     RSLAREVGKLGITVNAIAPGFIATELTQSLSDEARQRIAG"
     gene            complement(593..1975)
                     /locus_tag="VM910_39385"
     CDS             complement(593..1975)
                     /locus_tag="VM910_39385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVI68594.1"
                     /translation="MSPRESFALRDHLCPELKGRAISDAHHEVSLTDILHQSCLIGGL
                     RRLSGRSVLLAMSGQLLSALAMIELDGVARRMLLCPPDLNSEHIQALIEDAGIDAIVT
                     DEPPRWTDAGVYLIVGAHLPEQTIAVPRTERATEWLMLTSGTSGVPKIVGHTLDGLCG
                     AILADGLPRGPAPVWATFYDIRRYGGLQIFLRAVIGGGSMVLSEPGEALADHVARLAA
                     GGVTHISGTPSHWRKLLMSGSAAGFAPRYVRLSGEIADQAVLDGLAQAFPNASIGHAY
                     ASTEAGVGFAVNDGREGFPASLIGQNRDGVEMKVVDGSLRIRSSRTARAYVGKSPAAL
                     TDADGFVDTGDMVELRGDRYYFVGRRGGIINIGGLKVHPEEIEAVINRHPQVRMSRAK
                     SRRSPITGSIVVADVVLADGGENRTNEIRDEILRCCQTSLAPHKVPAVIRFVVTLDVT
                     PAGKLSRSDA"
     gene            complement(1962..2207)
                     /locus_tag="VM910_39390"
     CDS             complement(1962..2207)
                     /locus_tag="VM910_39390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVI68595.1"
                     /translation="MSVRLRIFAAMKRIAEEQQVALPQLNDDLLLHETGFDSLAFAIL
                     VARLEDDFGIDPFTISEDAAFPLTVGDFVRAYENVPA"
     gene            complement(2351..>2807)
                     /locus_tag="VM910_39395"
     CDS             complement(2351..>2807)
                     /locus_tag="VM910_39395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HVI68596.1"
                     /translation="RDAQGYFHFIDRVGDTFRWKGENVATSEVNDALRDCPGIVDATT
                     YGVTVPGCDGRAGMAAIVIEEDRFDLKTLADHLAQRLPVYAHPLFLRVSRMIDATETF
                     KQKKHQLAREGFDPATVSDPLYFRDPGSFAYRPLDASRFADIAKGGIRL"
BASE COUNT          538 a          896 c          887 g          486 t
ORIGIN      
        1 accggcgatg cgctggcgcg cttcgtccga gaggctttgc gtcaattcgg tcgcaatgaa
       61 acccggcgcg atcgcattca cggtgatgcc gagcttgccg acctcccttg cgagcgagcg
      121 cgtgaaaccg gtcgcagccg ccttggacgc gccgtagacg gacagcccgt tgtaaccggt
      181 cgaggcgatg atcgaggaga tgttgacgat acgcccctcg ccgtccgcca tcatgtgacg
      241 cacgacgtat ttggaaagga cgatcggcga cagcacgttg aggcgcacca acgcctcgat
      301 gtcggcattg tgcatcgtgg cgagcaggcc ttcgctgccg cctccggcgt tgttgacgag
      361 gccgtagatc gcgccgaact cgcgacgcac cgccttcacg aaatccggaa tcgcatcgac
      421 ctggctgagg tcgcaggcga ggaaatgaag accgccgtgc ccgagttcgg agatggccct
      481 gcgcagttcg ctgctttcgc gccgcgcgac cgcaatcacg ttgtagcccg cggtcgccag
      541 cctctgcgcg atcgccagac ctatgccgcg acttccgccg gtgaccagaa cgttacgcat
      601 cggagcggga gagctttccg gccggagtga cgtccagcgt gacaacgaag cggatcaccg
      661 cgggcacttt gtggggggca agcgaggttt ggcagcacct gaggatttcg tcacgaattt
      721 cgttcgtgcg gttctcgccg ccgtcggcga gcaccacatc ggcgaccacg atcgagcccg
      781 tgatcgggct gcggcgcgat ttggcgcgcg acatgcgcac ctggggatga cggttgatga
      841 cggcctcgat ctcctcgggg tgaaccttca atccgccgat attgatgatg ccgccgcgcc
      901 ggccgacgaa gtaatagcga tcgcctcgca attcgaccat gtcgccggta tcgacaaatc
      961 cgtccgcatc cgtaagcgcc gccgggcttt tgccgacata ggcgcgtgcg gtgcggctgg
     1021 aacggatgcg cagcgaaccg tcgaccacct tcatctcgac gccgtcgcga ttttgcccga
     1081 tcaggcttgc cggaaagcct tcgcgtccat cgttgacggc gaagccgacg ccggcttcgg
     1141 tcgaagcgta ggcgtggccg atagacgcat tgggaaaagc ctgcgccaga ccgtcgagca
     1201 ccgcctggtc cgcaatctct cccgacaggc ggacatagcg cggcgcaaag cccgcggcgg
     1261 atccgctcat caggagcttg cgccagtgcg atggcgtgcc agagatgtgc gtcacgccgc
     1321 ccgcggccag ccgcgcgacg tgatcggcga gcgcctcacc gggttcggac agcaccatgg
     1381 acccgccgcc gatcacggcg cgcaggaaaa tctgcaagcc gccgtagcgg cggatatcgt
     1441 agaacgtcgc ccataccggc gcgggaccgc gcgggagacc atcggcgaga atcgcgccgc
     1501 aaagaccatc cagcgtatgg ccgacgatct tcggcacgcc tgacgtgccc gacgtcagca
     1561 tcaaccattc cgtggcccgc tcggtccttg gcactgcgat cgtctgctcg ggcaagtgcg
     1621 ctccgacaat caggtacact cccgcgtccg tccagcgcgg aggctcgtcg gtgactatgg
     1681 catcaatgcc ggcatcctcg atcagtgcct ggatgtgttc ggaattgagg tcgggcgggc
     1741 acagcagcat ccgccgcgcg acgccatcga gctcgatcat cgcgagcgcg gagagaagct
     1801 gccccgacat cgccagcaac accgagcggc cggacagtct gcgaagaccg ccgatcaggc
     1861 agctttggtg caggatgtcg gtcagcgaca cctcatggtg cgcgtccgag atcgcgcggc
     1921 ctttcagctc cgggcagaga tgatcgcgaa gcgcaaaact ctcacgcggg gacattttcg
     1981 taagcccgga cgaaatcgcc gaccgtcagg gggaacgccg catcctcgga gatggtaaac
     2041 ggatcgatac cgaaatcgtc ttcaagccgc gcaaccagaa tcgcgaaggc cagcgagtcg
     2101 aaacctgtct cgtgcagcaa gagatcgtcg ttaagctgcg gcaacgcgac ctgctgctcc
     2161 tcggcaatcc gcttcatcgc agcaaaaatc cttaacctta ccgacatgga tcgagtcgcc
     2221 ttttccgctg taatcgtcgg ccgcgataat aaggcgtaaa ggtgtaagag tgccgccaga
     2281 cgctccaact ttcacgctgg ttggtaaaca tctcgcttgc cactttggga aatgtgctgg
     2341 gaaaatccgc ctaaagccgg attccgccct tggcgatgtc ggcaaagcgg gaggcatcga
     2401 gcggtcgata ggcgaaggac ccgggatccc tgaagtacag cggatcggac accgtggccg
     2461 gatcaaatcc ctcgcgcgcg agctgatgct ttttctgctt gaaagtttcg gttgcgtcga
     2521 tcatccggct gacgcgcagg aacagcggat gcgcgtagac cggcagccgc tgcgccaggt
     2581 gatctgcgag cgttttgaga tcgaagcggt cttcttcgat cacgatcgcg gccatcccgg
     2641 cgcggccgtc gcagccgggc accgtgacgc cataggtcgt cgcatcgacg atgccgggac
     2701 aatccctgag cgcgtcgttg acctcgctgg tcgcgacgtt ctcgcccttc cagcggaagg
     2761 tatcgccgac ccggtcgata aaatgaaaat agccctgcgc gtcgcgc
//
LOCUS       DATLJT010001736         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3643783,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001736 DATLJT010000000
VERSION     DATLJT010001736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643783"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1339
                     /locus_tag="VM910_39400"
     CDS             98..1339
                     /locus_tag="VM910_39400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVI68597.1"
                     /translation="MARLKFGAFLAPHHPLGEHPMLQFRRDLDFVEQLDELGYDEFWC
                     GEHHSSGWEMIASPEMFLAAAGERTKRIKLGTGVISLPYHHPFNVAQRMVQLDYMTGG
                     RAIFGSGPGALASDAHTLGIDPMTQRDRQDEAIAIIRRLFKGERVTAKSDWFTMQDAA
                     LQILPLQEEMPCVVASMISPSGMTLAGKYGIGIISLGSMSTKGLMSLAQQWEFAEDAA
                     KKNGTTVSRADWRVLLTWHVAESREQARREAGAGLMRWHNEYNVGTLMRPGLTAFKSP
                     DEAVDQTAFVDGAASAIGTPDDLVKLIKNVMKVSGGVGAIIGFVHDWANPENTRRSWD
                     LVARYVVPEINGYVESMRKSQKFVIENRAIFERAGEAVMAKIMGNEKAAAALADTGPG
                     RVAIPSVNAPDLQKEAAKRKA"
     gene            1508..2884
                     /locus_tag="VM910_39405"
     CDS             1508..2884
                     /locus_tag="VM910_39405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HVI68598.1"
                     /translation="MSMQNVAPAATPIRPPKRSALKHIPGDEGWPIIGKTLEVLADPK
                     GHIEKNAERYGLVYRSHLFGETSVVLLGPEANELVLFDQARQFSSTHGWDRILGLLFP
                     RGLMLRDFDDHRLHRKALSVAFKSGPMKSYLGELDRGIAARVAQWKAGPGEMLLYPAV
                     KQLTLDLAATAFLGAGIGPEVDEINRAFIDMVAAAVAPIRHPLPGTQMARGVKGRKRI
                     IAYFSEQIPVRRAGSGGDDLFSQLCQATHEDGALLSEQDIIDHMSFLMMAAHDTLTSS
                     LTSFVGELAAHPEWQDRLRGEVAALALAADAPTGMNHLEAMPLTEMAFKEALRLKPPV
                     PSMPRRAVRDFTFAGFAIPAGTMVGVNPLFTHHMPEIWPKPDTFDPLRFTEEAQRNRH
                     RFAFVPFGGGAHMCLGLHFAYMQAKCFARHFLQNLTVSLAPGYKPQWQMWPIPKPRDG
                     LRVTVKPV"
     gene            complement(2888..3394)
                     /locus_tag="VM910_39410"
     CDS             complement(2888..3394)
                     /locus_tag="VM910_39410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVI68599.1"
                     /translation="MTSMSATFASSPARDEARQLFLAVIAGFACAFALARVLPLAIDA
                     VSATIEPLCAASAGSALDTVEPIASYALPNVPGKRVTIVRVFYGPGGFSRPHRHAGSV
                     TAYITKGEIRSQLGGGPLETFEVGQSFFEPPGSTHLVSANASNTEPAELIAVFVADEG
                     AQLTTFIE"
     gene            complement(3391..>3565)
                     /locus_tag="VM910_39415"
     CDS             complement(3391..>3565)
                     /locus_tag="VM910_39415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68600.1"
                     /translation="GLPDDIAQAAVFLASDESSFVNGHDLVVDGAITGGRNWTQQQQG
                     YVALRKAFDQKTG"
BASE COUNT          679 a         1130 c         1145 g          611 t
ORIGIN      
        1 cgcaagcgat cgcccccgca ccgattgcct tgcaggtcgc cagcacgtag cttgccctcc
       61 cagagaacaa caaaattccg ctcgggagaa gaccagcatg gcgcgtttga aattcggagc
      121 ctttctcgcc ccgcatcacc cgctcggcga gcatccgatg ctgcaatttc gccgcgacct
      181 cgatttcgtc gagcaactcg acgagctcgg ctatgacgag ttctggtgcg gcgagcacca
      241 ttcgtcgggt tgggaaatga tcgcctcgcc tgaaatgttt ctggcggcgg ccggcgagcg
      301 caccaagcgg atcaagctcg gcaccggcgt gatttcgctg ccctaccacc atcccttcaa
      361 cgtggcgcag cgcatggtgc agctcgatta catgaccggc ggacgcgcga ttttcggctc
      421 gggtccgggc gcgctggcgt ccgacgcgca caccctcggg attgatccga tgacgcagcg
      481 cgaccgccag gacgaagcga tcgcgatcat ccgccgcctg ttcaagggcg agcgcgtcac
      541 cgcgaaaagc gactggttca ccatgcagga cgcagcgctg cagattttgc cgctgcagga
      601 agagatgcca tgcgttgtgg cctcaatgat ctcgccctcg ggcatgacgc ttgccggcaa
      661 atacggcatc ggcatcatct cgctcggctc gatgtcgacg aaggggttga tgtcgctggc
      721 gcagcaatgg gagtttgcgg aggatgcggc gaaaaagaac ggcacgacag taagccgcgc
      781 cgactggcgc gtgctgctca cctggcacgt cgccgagagc cgcgagcagg cgcggcgcga
      841 ggcgggcgcc ggcctgatgc gctggcacaa tgagtataat gtcggcacct tgatgcggcc
      901 gggactgact gcgttcaaat cgcccgacga ggccgtcgac cagaccgcgt tcgtcgacgg
      961 cgcagcctct gccatcggca cgccggacga tctcgtgaag ttgatcaaga acgtaatgaa
     1021 agtgtcgggc ggcgtcggcg ccatcatcgg ctttgtgcat gactgggcca acccggagaa
     1081 tacgcgccgg agttgggacc tggtggcgcg ctatgtggtg ccggagatca acggctatgt
     1141 cgaaagcatg cgcaagtcgc aaaaattcgt gatcgagaac cgcgccattt ttgagcgcgc
     1201 aggtgaagca gtcatggcca agatcatggg taacgaaaag gccgccgccg cgctggcgga
     1261 taccggtccg ggacgggtcg cgataccgtc ggtgaatgcg ccggatttgc agaaggaagc
     1321 cgcgaaacgg aaagcataag ggattcccgc aggcggaccg tcggcggcga caggccacaa
     1381 cctgtctcct ccggtaaagc agcgagattt tcgccgcaaa gccttgcgag aatttgcacc
     1441 gacgcgacga cagttctatt gggacgagac gatccgagcc ggccgatcgg ttgacggagc
     1501 aaacgccatg tcgatgcaga atgtggctcc cgctgcgact cccatcaggc cgcccaagcg
     1561 cagcgctttg aaacatatcc ccggcgacga gggctggccg atcatcggca agacgcttga
     1621 agtgctggcc gatcccaagg gccatatcga aaaaaatgca gaaagatacg ggctggtcta
     1681 tcgcagccat ctgttcggcg agaccagcgt cgtgctgctg ggcccggaag ccaatgaact
     1741 tgtcctgttc gaccaggcac ggcagttttc gtccacgcat ggctgggacc gcattctcgg
     1801 gctcctgttc ccgcgcggcc tgatgctgcg cgacttcgac gatcatcgcc tgcaccggaa
     1861 ggcgctgtcg gtcgccttca aatcgggacc catgaagtcc tacctcggcg aactcgacag
     1921 gggcattgcc gcacgcgttg cgcaatggaa ggcggggccc ggcgagatgc tgctttatcc
     1981 cgcggtcaag cagcttacgc tcgacctcgc cgcaactgcg ttcctcggcg ccggcattgg
     2041 acccgaggtc gacgagatca accgcgcctt catcgacatg gtggcggcgg ctgtcgcgcc
     2101 gatccgacat ccgctgccgg gcacgcagat ggcccgtggc gtcaaaggcc gcaagcgtat
     2161 catcgcctat ttctccgaac agatcccggt ccgccgtgcc gggagcggcg gcgacgatct
     2221 gttctcgcag ctttgtcagg cgacccacga agacggcgcg ctgctctccg aacaggacat
     2281 catcgatcac atgagcttcc tgatgatggc cgcgcacgac acgttgacgt cgtcgctgac
     2341 ctccttcgtc ggcgaacttg ccgcccatcc ggaatggcag gacaggctgc gcggcgaggt
     2401 cgcagccctc gccctcgcgg cggatgcgcc gaccggcatg aaccaccttg aggccatgcc
     2461 gctgaccgag atggcgttca aggaagcgct gcggctcaag ccgccggttc cctcgatgcc
     2521 gcgccgggcg gtgcgcgatt tcacgttcgc gggctttgcc ataccggccg gaaccatggt
     2581 cggcgtcaat ccgctgttca cccatcacat gccggagatc tggccgaagc cggacacgtt
     2641 cgatccgctg cgcttcaccg aagaggcgca gcgcaaccgc caccgctttg ccttcgtgcc
     2701 gttcggcggc ggcgcgcata tgtgcctggg gctgcacttt gcctatatgc aggcgaaatg
     2761 cttcgcgcgg catttcctgc aaaatcttac ggtatcactt gcacccggct acaaaccgca
     2821 atggcagatg tggccaatcc cgaagccgcg cgacgggctg cgcgtgaccg tgaagccggt
     2881 gtgacggcta ttcgatgaac gtcgtgagct gcgcgccctc gtcggcgacg aacaccgcta
     2941 tcagttcggc gggctcggtg ttgctcgcgt ttgccgagac cagatgcgtc gaacccggcg
     3001 gctcgaaaaa cgactgcccg acctcgaacg tctcgagcgg tccgccgccg agctgcgaac
     3061 ggatttcgcc cttggtgatg taggccgtga ccgagccggc gtggcggtgc gggcgggaaa
     3121 aaccgcccgg accatagaac acacgcacga tggtgacacg tttgcccggc acgttcggca
     3181 aggcatagga ggcgatcggc tcgaccgtgt cgagcgcgga gccggcgctg gccgcgcaga
     3241 gcggctcgat tgtggccgaa accgcgtcga tcgcgagcgg caacacccgt gccagcgcaa
     3301 aggcgcaggc aaacccggcg atcacggcga gaaaaagctg ccgcgcctcg tcgcgtgccg
     3361 gagacgatgc gaacgttgcg gacatcgatg tcatcccgtc ttttgatcga acgccttgcg
     3421 cagcgcgacg taaccctgtt gctgctgggt ccagttgcgg ccgccggtga tcgcgccatc
     3481 gaccacgaga tcatggccgt tgacgaacga agattcgtcg ctggccagga acactgcggc
     3541 ctgagcgatg tcgtcgggaa ggccc
//
LOCUS       DATLJT010001737         1649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3412342,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001737 DATLJT010000000
VERSION     DATLJT010001737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412342"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..772)
                     /locus_tag="VM910_39420"
     CDS             complement(26..772)
                     /locus_tag="VM910_39420"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HVI68601.1"
                     /translation="MTDALGWRMKFGVIAPSTNTVVQPEFDMMRPRGVTNHFARIHIP
                     NAPILGDADFEKLIAAVRGELLAAVDRVMTCEPGHLIMGMSSETFWDGLEGSERLRER
                     IEARAGVAVTMGSDAAGQALRRYGEIRRIGVVTPYMPIADEQVRRFFTDCGFEVARLV
                     GLKCASPVLIAHVGESELRDAVCAVDGPDVEAIVQVGTNLAMARLAAAGELWLDKPVI
                     AINTATYWWALRRNGIADPIDGLGTLLREH"
     gene            complement(896..>1649)
                     /locus_tag="VM910_39425"
     CDS             complement(896..>1649)
                     /locus_tag="VM910_39425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HVI68602.1"
                     /translation="LQRDIANLKAGLAKVKGVTGFLPVVAPASALPNAKNEHYRDEEA
                     FLFGLAEALGTEYRAIIDAGLDLQVDDAFIPYQYEKMVPPMTLADYRNWASRRIEALN
                     HALRGLPKDRTRYHICWGSWNGPHMFDVPIKDIVDLVLRVNVGAYSFEAANPRHEHEW
                     KVWKNVTLPAGKVLMPGLIAHSTNVVEHPDLVAERLLRYADIVGRDSVMAGTDCGFSQ
                     SPLAGRVHRSIMWAKLKCLAEGAAIASKVLWH"
BASE COUNT          288 a          551 c          510 g          300 t
ORIGIN      
        1 gggttgtcgg ttgttcgatc tggcgtcaat gctcgcgcag cagcgtaccg aggccgtcga
       61 tcgggtcggc gatgccgttg cggcgcaaag cccaccaata ggtcgcggtg ttgatcgcga
      121 tgaccggttt gtcgagccac agctcgcccg cggcggcgag ccgggccatc gcaagattgg
      181 tgccgacctg aacaatagcc tcgacatcgg gcccgtcgac ggcgcacacc gcgtcccgca
      241 attcgctttc gccgacatgc gcgatcaata cggggctcgc gcatttgagg ccgacgagcc
      301 gcgccacctc gaacccgcaa tcagtgaaaa accgccggac ctgctcatcg gcgatcggca
      361 tataaggcgt gacgaccccg atccgcctga tctcgccata gcggcgcagc gcctggcccg
      421 cggcgtccga gcccatcgtc acggcgaccc cggctcgcgc ctcgatccgc tcgcgcagcc
      481 gctcgctgcc ctccagcccg tcccaaaacg tctccgacga catgcccatg atcaggtggc
      541 caggctcgca ggtcatgacc cgatcgaccg ccgccaacag ctcgccgcgc acggccgcga
      601 tcagcttctc gaaatcggca tcgccgagga tcggggcgtt cgggatgtgg attcgtgcaa
      661 aatggttggt gacgccgcgt gggcgcatca tgtcgaattc cggctgcacg acggtgttgg
      721 tgctgggcgc gatgacgccg aacttcatcc gccatccgag cgcgtcggtc atttccaaat
      781 ctgccctaat cctcggcgag tccgagtgca tcgctgaaat atgcggcggg acttttcctg
      841 cggcgtgaca tcggcgaaga gctgcacgct gaactccacg ctgcaatccc gataatcaat
      901 gccagagtac cttcgaggcg atggcggcac cctcggcgag gcatttcagc ttcgcccaca
      961 tgatcgatcg gtgcacgcga cctgcgagcg ggctttgcga gaatccgcaa tctgtgccgg
     1021 ccataacgct gtcgcggccg acaatgtcgg catagcgcag cagccgctcg gcaacgaggt
     1081 cgggatgctc gacgacgttg gtcgaatgcg caatgagccc cggcatcagc accttgccgg
     1141 cggggagcgt cacgttcttc cacaccttcc attcgtgctc gtggcgtgga ttggcggcct
     1201 cgaaactata ggcgccgaca ttgaccctca gcacgagatc gacgatgtcc ttgatcggca
     1261 cgtcgaacat gtgcggtccg ttccagctgc cccagcagat gtggtagcgc gtgcggtcct
     1321 tcggcagccc ccgaagcgca tggttgagcg cctcgatgcg ccggctcgcc cagttgcggt
     1381 agtccgcgag cgtcatcggt ggcaccatct tttcgtactg gtacgggatg aacgcatcgt
     1441 ccacctgcag atcgaggccg gcatcgatga tggcgcgata ctcggtcccg agcgcctcgg
     1501 cgagaccgaa caggaacgcc tcctcatcgc gatagtgctc gtttttggca ttgggcagcg
     1561 cactcgccgg cgccaccacc ggcaggaatc cggttacgcc cttcaccttg gctaggcccg
     1621 ccttgagatt ggcgatgtcg cgctgcagc
//
LOCUS       DATLJT010001738        12198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4330580,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001738 DATLJT010000000
VERSION     DATLJT010001738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4330580"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VM910_39430"
     CDS             <1..842
                     /locus_tag="VM910_39430"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68603.1"
                     /translation="AIVSGELDISAMTAAAGLFNSVAKGAPLVIILDRGNNRPGFGYT
                     VTSVTEETYDQGVRSLADFGKLRGKRVGVPALGSINQYNVALALAKAGLDPAKDVQWT
                     VNVPQPDMMKMLGQKQVDVTDLAYQFGFFAQNNKWGPMIATGDQVAPGTAIAAFAARK
                     DYLQKNRDVVVRWAIAYLQGVKEFNAAAVAPDKHPDVVDILARTTALNKPELIKAIAP
                     HWSYVNEDGAPPVDSIMTMQDFWSGRDFHFVEQKVSREQLFDLSIANEAKARLGREKP
                     FGN"
     gene            887..2296
                     /locus_tag="VM910_39435"
     CDS             887..2296
                     /locus_tag="VM910_39435"
                     /inference="COORDINATES: protein motif:HMM:NF005687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HVI68604.1"
                     /translation="MNTELWRWPASNLAQAISTRQISSREAVTSCLVRLEEVNPRINA
                     VVDVMADDALAAADHADEIIARREAAGPLHGVPVTIKINVDCVGRTTTNGVAAFQDRI
                     AKTDSPPVANWRKAGAIVIGRTNVPPFSARFFTDNALYGRTLNPWDVARTPGGSSGGA
                     AAAVATGIGPLAHGNDRAGSIRYPAFACGVAGLRPTIGVVPTFESTEMADPTITTQLT
                     HVQGPLARSVGDVRLGFLAMAGHDSRDPWWVPAELNAGTAVGSVRVAMLAHTSGTEID
                     QAIYATIRDAARWLEDAGYRVEEATPPRLEEAAEMFFTLIRTEEKAGTSKAIERFGDD
                     RLRRARASTMAYAGEFDLEGYIQAFARRASILPEWMLFFERYPLLLLPVSWERPFPVD
                     FDQQGDNAVRRMLTAHHPMLAISILGLPALAIPTGSADGIPVGVQLVAARFHEHVCLR
                     AGEAIEARYKVMTPIDPRF"
     gene            2358..3779
                     /locus_tag="VM910_39440"
     CDS             2358..3779
                     /locus_tag="VM910_39440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI68605.1"
                     /translation="MGRVLIENGYVVTVDPQRRVYPDGFVLINGNKIEAVGNSAQMPT
                     RDVARTIDASGMIVIPGLINAHQHFYYHLFKGLGHGLLLEDWFPHLVFPVLPHLTDDD
                     MELTSYLAAIEMLSTGTTCCLNHLRTTTSEELLQRISAPTAELGFRQVIGKEVQCRLA
                     GNPRHPRDLADEIAYVEDLIPRWKHTHDGLTRLCLVAECTSVFVEHKLTSEELLIGSK
                     KLADRHGLKLAAHISGGTLSFDKSYLQILRKTGQTDAQLLMQMGLLDSSWILVHGINC
                     TPTDLRLIANSGASLIYCPTSEAVRGGGIGPAAAALAVGVNVALGSDGPMVDDSVDML
                     EQMKACSFLQGVKHLDPTIMPPERCIEMATINAARAMGLDDEIGSLEVGKLADVAIFD
                     LDTPHSTPATNPVASLVYSARGPDAHTVFVEGREVVTNHKLTTFSDTKHLFARARART
                     QEIVTKPACSTGRHRPGPSRFRS"
     gene            3799..4863
                     /locus_tag="VM910_39445"
     CDS             3799..4863
                     /locus_tag="VM910_39445"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68606.1"
                     /translation="MKQISRRRFVARVSSAGMVLLAGSKRVRAQGHEKLTIGLSPFIN
                     QATIFLANELGYFSKMGLDIDMKIFMDGALVVAPMLSGELQIGIMTPNAGFFNSLSRG
                     GPLRAFLCNGQGRRGRAVTALVIRSDHYDAGIKTLADLARMKGKIAAVGAAGSINQYG
                     LGSALKLAGLDPVRDVQWQTSVPQPDIVKQLGQKQVDVAEITYHLAYLAQKEGFCRIL
                     WSRDEILPDSQAAMHTVRDDILQEKRDTVVKYAMACIQAGRVFNQVAGDPEKHADMLK
                     LIVKSIFPRDEALLKAVAPHWEWIAEDGMPNVTSIMAEQDFWADTFKTVETKVPQQRV
                     VELSIAKEAVQRLSVERPFG"
     gene            complement(5072..5516)
                     /locus_tag="VM910_39450"
                     /pseudo
     CDS             complement(5072..5516)
                     /locus_tag="VM910_39450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(5540..6382)
                     /locus_tag="VM910_39455"
     CDS             complement(5540..6382)
                     /locus_tag="VM910_39455"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HVI68607.1"
                     /translation="MTWDRQRRLAEIFRRAHQPPPLLLLPNAWDAMSARQFEAAGFGA
                     IATTSGGLAWALGFPDGERAPWQEVVAATARIVRAVRVPVTADIEAGYGDTPDRVGTR
                     VAEILQAGVFGFNIEDGTPSPDRPVRAIEDASARIRVARAAANAAGVPAVINARVDLY
                     LKNIGDPESRLAETVWRSKAYLAAGADCIYPFGLADVGTIARLTQALDGAPVNIVARP
                     DTSLPQLERIGIARVSIASGAALTVMSLIKQIGEELYVSRRFDVLKHSISRVEAQKLF
                     EPRD"
     gene            complement(6502..7428)
                     /locus_tag="VM910_39460"
     CDS             complement(6502..7428)
                     /locus_tag="VM910_39460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68608.1"
                     /translation="MVQPSSYAFVPLPDRAPLKFPDGARVALIFTINIEYWEPFRPGQ
                     KEPLFPGGPATIPHALPGDVLDTANWTWREYGHRVGIWRLMDAFDTLGIAPSCTCNGM
                     ILQERRRIIDAVKERGWELVPHNWVQNDLLTYYAYKPNEERAVIRRTLDKYAEVVGKP
                     AKAWLSSAIRGTAHTPAFLKEFGLIAYCDYLNDDQPYLIDTVHGPIVCVPYSNDINDF
                     NMFARGGLSTRDGVDMLRLCFDQLYAEGARSGRIMNVGLHPHVIGQPYRIAALREFIE
                     YIKGQSNVWFPTREQIALWYLGEAGQHMPGKA"
     gene            complement(7428..8414)
                     /locus_tag="VM910_39465"
     CDS             complement(7428..8414)
                     /locus_tag="VM910_39465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68609.1"
                     /translation="MRFAAKLLIEPNKRPFTIMLAVVIVIALMDQGEGYFFSLGTVFS
                     VMQLFATFGLVALGLGLSMLVREFDLSVAGIVGLAGCIAVMTGVSNPWLGVLFGVGAG
                     VVTGVIQGLIMTRLHLSSVGVTLGGLLTLQGLTYVLTENKTISYPNMAVALGLNAPIG
                     GLVSVRSVAALAVFVIAALAMTYTHIGRDIIATGSDRRASRVAGLKTDRVIISVFAAS
                     GASAALAGVLLSYSLGAASPVALADVLPTAAAASIVGGVSVVGGRGSPLGIAAGVLTL
                     CILRSGLSAIGIEPHVHDLVTGAILLAIAVLDAPELVQRATAWRLDRAERKA"
     gene            complement(8417..9298)
                     /locus_tag="VM910_39470"
     CDS             complement(8417..9298)
                     /locus_tag="VM910_39470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVI68610.1"
                     /translation="MAGGAIAAFALSTPGFTSPLSINALLNAISLIGCVAIGMTFITL
                     TGNIMSLCLGATVSASALIFLASLSFGVFPAFLIAILFGVVITAVQGFLVGSIRANPI
                     LVSIAGLSLLIGGAALITGGQRIYPSGEGLQMFKGRVAGVPIEALYFFATVVIGQLVL
                     SCTRIGREMVMAGSNLRAATAAGIGTTRAITCAYVIAGACSAVSGVLLAARYGSGDME
                     LGAGYDYTAIAAVLVGGTSIQGGSGSALRTLVGVSVIAIIEVVLLLRGFSQQLQYLIT
                     GIIVLLVIMLHTIGERH"
     gene            complement(9373..10866)
                     /locus_tag="VM910_39475"
     CDS             complement(9373..10866)
                     /locus_tag="VM910_39475"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HVI68611.1"
                     /translation="MPILAVDGVTKRFGPNEVLKNVSLSLGAQEIRAICGENGAGKST
                     LVKIITGVHAADGGTVLVDGTPTEITDPQHAQELGIALVAQELSLAPALSVFDNIWLG
                     NREVPLFHRRKELRERARAALEKVGLEELPLEAPVSRLSIGRRQLVELARMLTRDARV
                     FILDEPTATLSDVEIERIFAALRALKDEGKSVIYITHRLGEVFQICDSVSVLRNGELV
                     GTRRTAEIDRAQLMEMMLGRSLREMYPRYDSRPGQPMLEVRDLWVPPVVQNFNLTAQR
                     GMVICLAGQVGSGATEVLRALAGLAFDSTGEVGIAGQPVQLRSVMRAQRAKMQFISED
                     RAGEGVFLRLNVGQNLVATRLGDHSRLGILARRGLRATAAKLAAAVGVDRARLRSQAD
                     ELSGGNQQKVAFGRVIGGDEPGVLLMNEPTRGVDVGARADIYRLIRQFCDRGYCLVMA
                     SSDIEEVLGMADVIVSMYRGRQVATYQRGEADLHRVLADITHPVAAA"
     gene            complement(10873..11919)
                     /locus_tag="VM910_39480"
                     /pseudo
     CDS             complement(10873..11919)
                     /locus_tag="VM910_39480"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            complement(12014..>12198)
                     /locus_tag="VM910_39485"
     CDS             complement(12014..>12198)
                     /locus_tag="VM910_39485"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavin-dependent monooxygenase"
                     /protein_id="HVI68612.1"
                     /translation="KEVIETAYHFAGGTAIFENQPFERRLRDVHAATQQVQAQFVNFE
                     VAGQVLLGLPVTSKLI"
BASE COUNT         2391 a         3865 c         3705 g         2237 t
ORIGIN      
        1 cggcgatcgt ctcgggcgag cttgacatct ccgccatgac cgcggccgcc ggcttgttca
       61 atagcgtggc caagggagcg ccgctcgtaa tcattcttga ccgtgggaat aatcgtccgg
      121 gtttcggcta taccgtcacc agcgtcaccg aggagacgta cgatcaaggg gttcgatcgc
      181 tggcggattt cggcaagctc aggggtaagc gcgtcggcgt tccggctctc ggcagcatca
      241 atcagtacaa tgtcgcgctc gcgctcgcca aggcgggcct cgatccggct aaggatgtgc
      301 agtggaccgt taacgtgccg cagcccgata tgatgaagat gctcgggcaa aagcaggttg
      361 atgtcactga ccttgcctac cagttcggct tcttcgccca gaacaataaa tggggcccga
      421 tgatcgccac gggcgaccag gttgcccccg gcacggcaat tgccgctttt gcggcgcgca
      481 aggactattt gcagaagaat cgggacgtgg ttgtccgctg ggccatcgct tacctgcagg
      541 gcgtgaagga gttcaatgcc gcggctgttg ctcccgacaa gcatcccgac gtcgtcgata
      601 tcctggcgcg taccacggcg ctgaacaagc cggagctgat caaagcgatc gccccccatt
      661 ggagctatgt caacgaggac ggcgccccgc cggtcgattc gatcatgaca atgcaagact
      721 tctggagcgg cagagacttc cactttgtcg agcagaaggt ttcacgcgag cagcttttcg
      781 atttgtcgat cgccaatgag gcgaaggcgc gcctgggtcg cgagaaacca ttcggcaact
      841 gagcggccgg tgaggaattg ttagaaactg ctcccgtgcg gttcctgtga acactgaact
      901 ctggcggtgg ccggcgagca atctcgcgca agccatcagc acgcgtcaga tctcaagccg
      961 cgaggctgta acgagctgcc tcgtccgcct cgaagaggtc aacccgcgca tcaacgccgt
     1021 ggtcgatgtg atggcggacg atgctctcgc tgcagccgat cacgcagatg agatcatcgc
     1081 ccggagagag gcagccggac cgctgcacgg cgtccccgtc actatcaaga tcaacgtgga
     1141 ctgcgtcggt cgtaccacca ccaacggggt ggccgcgttc caagaccgta tcgccaagac
     1201 cgacagcccg ccggtcgcga actggcgcaa ggccggcgcg atcgtgatcg gccggaccaa
     1261 tgttccgccg ttcagcgcgc gtttcttcac cgacaatgcc ctctacggtc gcacgctcaa
     1321 tccctgggat gtggcgcgca cgccgggcgg ctcgagcggt ggtgctgccg cggccgttgc
     1381 gaccggcatc ggcccgctcg ctcatggcaa tgatcgggcg ggctccattc gttatccggc
     1441 gtttgcctgc ggcgtggcgg gcttgcggcc aactattggt gttgtcccga cgttcgaatc
     1501 gacggagatg gccgatccca ccatcacaac gcagctcacc cacgtgcaag gtccattagc
     1561 gcgatccgtc ggcgatgtgc ggctgggctt cctcgcgatg gctgggcacg attcgcgcga
     1621 tccctggtgg gtcccggcgg agcttaatgc gggcaccgcg gtaggatcgg tgcgcgtagc
     1681 catgctcgca cacacgtcgg gcactgagat cgatcaggcc atctacgcga ccatccgaga
     1741 tgctgcgcgc tggttggagg atgccggcta cagggtcgaa gaagcgacgc ctccgcgcct
     1801 ggaagaagcg gctgagatgt tcttcacgct tattcggacc gaggagaagg ctgggacgag
     1861 caaggcaatc gagcgtttcg gcgacgaccg acttcggcgc gccagggctt ccaccatggc
     1921 gtatgcgggt gaattcgatc tcgaaggcta tatccaagca ttcgcgcggc gagcttcaat
     1981 cctgcccgag tggatgctgt ttttcgagcg ctatccgctt ctgctcttgc cggtctcctg
     2041 ggaaaggccg tttccagtcg acttcgacca acaaggcgac aacgcagtca ggcggatgct
     2101 caccgcgcat catccaatgc tcgcgatctc gatcttggga ctgcccgcgc tcgctatccc
     2161 gaccggttcc gcagatggaa taccggtcgg ggtgcaactc gtcgcagcgc gatttcacga
     2221 gcatgtttgt ttgagggcag gggaagccat tgaggcgcgg tataaagtca tgacccccat
     2281 cgatccgcgc ttttgatgcc gctcagccca ccggaaactc gtaatatgtg ctttgctatc
     2341 gaagggaaac ttggggcatg ggcagagttc tgatcgagaa cggttacgtc gtcaccgtcg
     2401 atccccagcg gcgggtgtat cctgacggat ttgtcctcat caacggaaac aagatcgagg
     2461 cggtcggcaa ttcagcgcag atgccgacgc gggatgttgc ccgcacgatc gatgcgagcg
     2521 ggatgattgt cattcccggg ctcatcaacg cgcatcagca tttctattat cacctgttca
     2581 aaggcttagg acacggcctt ttgctggagg actggtttcc gcatctggtg tttccggttc
     2641 tgccccatct cacggacgac gatatggagc tcacttccta tctcgccgcg attgagatgc
     2701 tgagcaccgg aacgacctgt tgcctcaacc atctgcgtac caccacgagc gaggagctat
     2761 tgcagcgcat ttcggcgccg acggccgagc tcggctttcg gcaagtcatc gggaaggaag
     2821 tccagtgccg gctggccggc aatccgcggc atccgcgcga tctcgccgac gagatcgctt
     2881 atgtcgaaga ccttatcccc cggtggaagc acacccacga cgggttgacg cggctctgtc
     2941 tcgtcgcgga atgcacctct gtcttcgtcg agcataagct cacgtcggaa gaattgttga
     3001 tcgggagcaa gaagctcgcc gaccgacatg gtctgaagct cgcggcccat atttcgggcg
     3061 gtaccctgtc attcgacaaa agctatctgc aaatcctgcg caagaccggc caaaccgatg
     3121 cgcaacttct catgcagatg gggcttctcg attcctcctg gattctggtg cacgggatca
     3181 attgtacgcc gacggacttg cgtctgattg ccaacagcgg agcaagtctc atttactgtc
     3241 cgacaagtga ggccgtgcgg ggtggcggca ttggtcccgc cgctgccgcg ctcgctgtcg
     3301 gcgtgaatgt cgcacttgga tcggacggtc ccatggtcga tgactctgtc gacatgctgg
     3361 agcagatgaa ggcttgttcg ttcttgcagg gggtcaagca tctcgatccg accatcatgc
     3421 caccggagcg atgcattgaa atggccacca tcaatgccgc ccgcgcgatg gggctcgatg
     3481 acgagatcgg gtctcttgaa gtgggaaaac ttgccgacgt tgccattttc gacctcgata
     3541 caccgcattc aacacccgca accaatccag ttgcgagtct tgtctattcg gcccgaggac
     3601 cggacgcgca tacagtgttc gtggagggcc gcgaggtggt cactaatcat aagctcacga
     3661 ctttttccga taccaagcat ctgttcgcgc gtgcacgcgc caggactcaa gagatcgtaa
     3721 caaagccagc ctgttcgacc gggcggcatc ggcctggacc aagccgcttc cgcagctaac
     3781 cgaaaggatc gcgacatcat gaaacagata tctcgaagaa ggtttgtggc cagggtcagc
     3841 tccgcaggaa tggttttgct cgcgggcagc aaaagggtga gagcgcaagg acacgaaaag
     3901 ctgaccatcg gcctgtcacc attcattaat caggccacga tctttttagc caatgagctt
     3961 ggctattttt ccaagatggg tttggacatc gacatgaaaa ttttcatgga tggcgccttg
     4021 gtggtggcgc cgatgctgtc gggtgagctt cagatcggaa tcatgacccc caacgcaggc
     4081 ttcttcaact cgctctctcg cggtgggcca ctccgcgcct ttctttgcaa tggccagggc
     4141 cgccgcggcc gcgcggtcac cgcccttgtc attcgcagcg atcattatga tgccggcatc
     4201 aagacgttgg cggatcttgc gcggatgaaa ggcaagattg ctgcagtcgg ggcggcaggc
     4261 agcatcaacc aatatggact gggttccgct cttaagctcg ctggtctcga tcccgtgcgc
     4321 gacgtgcagt ggcagacctc ggttcctcag ccggacattg taaaacagct cgggcagaag
     4381 caggttgatg ttgcggaaat tacctatcat ctcgcctatc tcgcccaaaa ggagggcttc
     4441 tgccgaatct tatggtcacg cgacgaaatc ctgccggact ctcaggcggc gatgcatacc
     4501 gttcgcgatg acatcttgca ggaaaagaga gacacagtcg taaaatacgc gatggcgtgc
     4561 atccaggcgg gacgtgtgtt caatcaggtc gcgggtgatc cggaaaaaca tgcggatatg
     4621 ctcaaactga tcgtcaaatc catatttccg cgcgacgagg ctttgctcaa agcggtcgct
     4681 ccacattggg aatggattgc cgaagacggc atgccgaatg tcacttcgat tatggccgag
     4741 caggatttct gggcggatac cttcaagaca gtcgaaacga aggtgccgca acagcgcgtg
     4801 gtcgagctca gcattgcgaa agaggccgtg cagcggctca gcgtggaaag acctttcgga
     4861 taaatcgctg gctcatgaaa caagtgttag gcttcgggtt tggccattca ccctcccagt
     4921 tgaccgacaa caagacagaa caatgcatgt tcgcccctcg cacgtcacgc ggtagcgtag
     4981 cgacttgaag ttgacccatt caagtccgtc gaatgcggca atggccgtcg gggtcacgcc
     5041 gcgacccgcg ggcgtcgcga actgaaaccg gctattcagc tgcccgtcgc cgcacttgat
     5101 ggccgccggg caccctacga aaggcgatgt tgagccggtt ggcgccattg atggcgacga
     5161 gcctcttcgg tgaactgttt acgcgcctcc tcataaacct cgtcgggcac ttgagtgtca
     5221 gcgactaagg tgaccgcctc ggtccaggcc agtgccgcac gctcacggtc gctgtagaat
     5281 ggcgaatcgc gccaggcgtt caacagatag agccgctgct cgctttcccc gtcggcacgc
     5341 aattccttcg tatgcatgtc gatacagtgg gcgcatccgt tcatctggga tgcccgcagc
     5401 ttgacgagct ctaaaagcga acgatcgaga ccgcagttgc gcacgaacat gtgcagggcc
     5461 ttcatggcgt tctcgccttg cggagaagct ttgcggaaat ccaatcgagc tttcattgca
     5521 ggcctccatc tcgcgagatt caatctctcg gttcaaacag cttttgtgct tcgacgcggc
     5581 tgatcgaatg cttcagcacg tcgaagcgac ggctgacgta cagctcttcc ccgatttgct
     5641 tgatcagcga catgaccgtc agcgcagcac cagaagcgat gctcacgcgc gcgataccga
     5701 tgcgctcgag ctgcggcagc gaggtgtccg gcctggccac gatattgacc ggcgcaccgt
     5761 cgagcgcctg ggtcaaccgc gcgatggtgc caacatcggc aagaccgaac ggatagatgc
     5821 aatccgcccc ggctgccaga taagccttgc tccgccacac ggtctctgcc aagcggcttt
     5881 ccgggtcgcc gatgttcttg aggtagagat cgacgcgggc attgatgacc gccggcaccc
     5941 ctgccgcgtt cgcggctgcg cgcgcgacgc gaatgcgggc actggcatcc tcgatcgcgc
     6001 ggaccggcct gtctggagaa ggcgtgccat cctcgatgtt gaagccgaaa acgcctgctt
     6061 gcaggatttc ggccactcgc gttcccaccc gatcgggcgt gtcgccgtat cccgcctcga
     6121 tgtcggcggt gaccggcaca cgaactgccc gcacgatccg cgcagtcgcc gccacgacct
     6181 cctgccaagg cgcccgctca ccgtcaggaa aacccaaggc ccaggcaagg ccaccgctgg
     6241 tcgtcgcaat cgcgccgaag ccagccgcct cgaattggcg cgcactcatg gcgtcccagg
     6301 cgttgggcag aagcaatagt ggaggtggct gatgggctcg ccgaaaaatc tccgccagtc
     6361 ttcgttgccg atcccatgtc atctgcggga gcgtaggtcg ggcacgtgca tctcacaaga
     6421 cctgcgctcc ggccccacct cgcttcagca agatcggccg atcgcttgaa cctcatgccg
     6481 gctttcgagg caaacgagcg tttaggcctt tcccggcatg tgctgccccg cttcgccgag
     6541 ataccacaag gcgatctgct cgcgggtcgg aaaccaaacg ttcgattgtc ctttgatata
     6601 ttcgatgaac tcgcgcagcg cggcaatccg gtacggctga ccgatgacat gcgggtgcag
     6661 gccgacgttc atgatccgcc cgctgcgtgc gccttcggca taaagctgat cgaaacagag
     6721 cctgagcatg tcaaccccgt cgcgcgtcga aaggcccccg cgggcgaaca tgttgaagtc
     6781 gttgatgtcg ttcgaatacg gcacgcagac gatcggccca tggacggtat cgatcagata
     6841 gggctgatcg tcattgaggt agtcgcaata ggcgatcaag ccgaactcct tgaggaaggc
     6901 gggcgtgtgc gccgtgccgc ggatcgccga cgacagccag gccttcgccg gcttgcccac
     6961 cacctcggcg tacttatcga gcgtccgacg aatgacggcc cgttcctcgt tcggcttgta
     7021 ggcgtagtac gtcagcagat cgttctgcac ccagttgtgc ggcaccagct cccagccgcg
     7081 ctccttcact gcgtcgatga tgcggcggcg ctcctgcagg atcatgccat tgcaggtgca
     7141 ggacggcgcg atgccaagcg tgtcgaatgc gtccatcaac cgccagatgc cgacgcggtg
     7201 gccgtactcg cgccacgtcc agttcgccgt atcgagcacg tcgcccggca gggcatgggg
     7261 aatggttgca ggtccaccgg gaaatagcgg ctccttctgg cccggccgga acggctccca
     7321 gtactcgata ttgatggtga agatgagcgc cacacgcgca ccatccggaa atttcagcgg
     7381 cgcgcggtcg ggcagcggca cgaacgcata gctcgaaggc tgcaccatca cgctttcctt
     7441 tctgcgcggt cgaggcgcca ggcggtggcg cgctgcacga gctcgggcgc gtcgagcacg
     7501 gcgatggcaa gcagaatagc accggtgacg agatcatgga cgtgcggctc gatcccaatc
     7561 gcgctcaggc cagagcgcaa aatgcacagc gtcagtacgc ctgcggcaat tccgagcgga
     7621 ctgccgcggc cgccgaccac ggacacgccg ccgacgatcg aggccgcggc cgccgtcggc
     7681 agcacatcgg cgagcgcgac cggcgatgcc gcgcccaggc tgtaactcag cagcacgccg
     7741 gccaaggcag ccgaggcgcc cgaagccgca aacacgctga tgatcacccg atcggtcttg
     7801 agacctgcga cccggctcgc ccggcgatcg ctgccggtgg cgatgatgtc gcggccgatg
     7861 tgggtgtagg tcatcgctaa ggctgctatg acgaaaaccg ccaatgccgc aacgcttcgc
     7921 accgaaacga gacccccgat cggcgcattg agccccagcg ccactgccat gttgggatag
     7981 ctgatagtct tgttttcggt gagcacgtag gtgaggccct gcagcgtcag cagcccgccg
     8041 agcgtgacac cgaccgagct cagatgcagc cgtgtcatga tcaggccctg gatcacgccg
     8101 gtgaccacac cggcaccgac cccgaacagg accccaagcc atgggttgga cacgccggtc
     8161 atcaccgcga tacagccggc aagcccgacg atgcccgcga ccgagaggtc gaattcacgc
     8221 accagcatgg acaggccaag gccaagcgcg acgaggccga aggtggcaaa cagctgcatc
     8281 acgctgaaga cggtgccaag actgaagaaa tagccctcgc cctggtccat cagggcgatc
     8341 acgatgacga ccgcgagcat gatcgtgaat ggccgcttgt tcggctcaat cagcaatttg
     8401 gccgcgaacc gcaccgctaa tgcctctcgc cgatggtgtg cagcataatg accagcagaa
     8461 cgatgatacc ggtgatcaga tattgcagct gctgactgaa accgcgcagc aacagcacca
     8521 cttcgatgat ggcaatgacg ctcacgccga caagcgtgcg cagcgctgag ccggaaccgc
     8581 cctggatgct ggtgccgccg accaacaccg cggcaatggc ggtgtagtcg tagcccgcac
     8641 cgagctccat gtcgcccgaa ccgtaacgtg ccgcgagcaa aacgccggaa accgccgagc
     8701 aggcgccggc gatgacataa gcacatgtga tggcccttgt cgtcccgatg ccggcagccg
     8761 tggcagcgcg gaggttactg ccggccataa ccatctcgcg gccaatccgc gtgcaggaca
     8821 gcacgagctg gccgatgacc acggttgcga agaagtacag cgcctcgatc ggaacaccgg
     8881 cgacgcggcc cttgaacatc tgcaggcctt cgcccgaggg atagatgcgc tgaccgccgg
     8941 tgatcagcgc cgcgccaccg atcagcagcg aaagcccggc gatgctgacc aggatcggat
     9001 tggcgcggat cgaaccaacc aggaagccct gtaccgcggt aatgacgacg ccgaacagga
     9061 tggcaatcaa aaatgcgggg aacacgccga acgacaggct ggcgaggaaa atcagcgccg
     9121 aggccgatac ggtggcgcca aggcagaggg acatgatgtt gccggtcagc gtgatgaagg
     9181 tcatgccgat ggcgacgcag ccgatgagcg agatggcgtt gagcaatgcg ttgatgctca
     9241 ggggcgaggt gaagcccggc gtgctcagcg cgaaggcagc gatcgccccg ccggccacga
     9301 tgcggacagc tagcaccaca tgccgcgctc gcgccccgcg cagacggaac cgcgcacgcg
     9361 gcaatagctc gctcatgcgg ccgcaaccgg atgcgtgatg tcggccagga cgcgatgcag
     9421 gtcggcctcg ccccgctggt aggtcgcgac ctgccggccg cggtacatgc tgacgatgac
     9481 gtcggccatg ccgagcacct cctcgatatc ggaggacgcc atgacgagac agtagccgcg
     9541 gtcgcagaac tgccggatca gccgatagat gtcggcgcgc gccccgacat cgacgccacg
     9601 cgtcggctcg ttcatcagca ggacgccggg ctcgtcgccg ccgatcacgc gaccgaatgc
     9661 aaccttctgc tggttgccgc cgctcagttc gtcggcctgc gagcgcagcc gcgcgcggtc
     9721 gacgccaact gccgcagcga gcttcgccgc tgtggcgcgc aaaccccgcc gggccaaaat
     9781 gccaagccgg ctgtgatcgc caagcctggt ggcgactaaa ttctgaccga cgttcagccg
     9841 aaggaacacg ccctcgccgg cgcgatcctc ggagatgaat tgcatcttcg cacgctgggc
     9901 cctcatcacc gagcgaagct gcacgggctg cccggcgatg cccacctcgc cggtcgagtc
     9961 gaacgcaagt ccagccagtg cccgcaacac ctcggtggcg ccggacccga cctggccggc
    10021 gaggcagatc accatgccgc gctgcgccgt cagattgaag ttctgcacca ccggcggcac
    10081 ccaaagatcc ctcacctcca gcatgggctg gccgggccgg ctgtcatagc gcggatacat
    10141 ctcgcgcaaa ctccgcccga gcatcatctc catcagctgc gcgcgatcga tctctgccgt
    10201 ccgccgcgtc ccgaccagct cgccgttgcg caacaccgag acgctgtcgc agatctggaa
    10261 cacctcgccg agccggtggg tgatgtagat caccgacttg ccctcgtcct tgagcgcgcg
    10321 cagcgccgca aaaatgcgct cgatctcgac gtcggaaagc gttgcggtcg gctcatccag
    10381 aatgaacacc cgcgcatcgc gggtcagcat ccgcgccagc tccacgagtt gccgccgacc
    10441 gatgctcaat ctcgatactg gcgcctcgag cggcagctcc tccaacccca ccttctccag
    10501 cgccgcccgc gcccgttcgc gcagctcctt gcggcggtga aacagcggca cctcgcggtt
    10561 gccgagccag atgttgtcga acaccgaaag cgccggcgcg agactgagct cctgcgccac
    10621 cagtgcgata ccgagttcct gcgcatgctg cgggtcggtg atttcggtcg gcgtcccgtc
    10681 gaccagcacg gtgccgccgt cagcggcgtg cacgccggtg atgattttga ctaacgtgct
    10741 cttgcccgcg ccgttctcgc cgcagatcgc gcggatctcc tgcgcgccga gcgaaagcga
    10801 gacgttcttg aggacctcgt tcgggccgaa ccgctttgtt accccgtcaa cggcaagtat
    10861 cggcatcgaa gctcaatacg agcagccggc ttcgaactgc gccaggttgt ccttggtgat
    10921 gccgtgtacc ggcaccagct cgtatttctg cagcttctgg ccattgaagt agcccgcgat
    10981 cttcttcgcc gctacttcag cctcttcggt cgggatttgc gtcgcggtcg cgtattgctg
    11041 gcccgacttg atgtgtacca tgccgtcctt catgcagttg ctggcgacca ccacgatgcc
    11101 cttgttggca acaccgagcg ttagcccggc cgactccacc gcctggatcg agcctgtcgc
    11161 ctggttgtcg gccatggcga agatgccgcc gatcccgccg cggccgctga agcgcacgag
    11221 cagctctcca gtgatcttct cggtcatcgc cgtattccac ttgccgtctt cggtggcaac
    11281 aagctcgatg cccgggtgct tgctcagcac ctccttgaac gccgccatgc gcgtctggac
    11341 gttgagctgc taggcgacgc ccgtgacggc cacgacctga gccttcgtct tgccctccgc
    11401 ctgcaggcct ttgaccatgc cctccgcggc gagacgccca agctcggcgt gatcggtccc
    11461 gatatacgag gtgaacagct cctcgtactc cttcttcagc ggagtgacga tcagcaccac
    11521 cggaatgccg gcggccttgg cccgcgtcaa cgccgggatg atggcctggt cgttgacgta
    11581 cacgatggcg atgaggtcga acttctgcgc gatcgcgtcg tcgacctgct gcgactgcac
    11641 ggcggcgtcg tagttcgaac tgagattcgt taccttcatg ccgtcctgcg tcgcgaactt
    11701 cgtgaaggtc gaggtccagg cgccgatgta aggattggta ttggcggtgc cgagcggcat
    11761 cacacgcttg ccctcgggcg acgcaatggc gtcgtgcccg gttacaactg ccagcagaag
    11821 cgcgcacaga cccaccacga agcgcatgaa catctcctgt tccaagacca agatcccaaa
    11881 aatgttagcg tcagtcgggc aagcgcacca gcaggccatg cgacgcagga ttgccgaaat
    11941 gaccttctgc aagccagcgc gcacaggcgg ttagccgctc gtccgcgcgc caaacggccg
    12001 agccattgca acatcagatc aatttcgacg tgaccggaag tccgagcagc acctgaccgg
    12061 ccacctcgaa gttgacgaac tgggcctgca cctgctgcgt cgcggcgtgc acatcacgca
    12121 gccgccgttc gaacggctgg ttttcgaaaa ttgccgtgcc gccggcgaag tgataggcgg
    12181 tctcaatcac ctccttcg
//
LOCUS       DATLJT010001739         1768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1346988,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001739 DATLJT010000000
VERSION     DATLJT010001739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346988"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VM910_39490"
     CDS             <1..1179
                     /locus_tag="VM910_39490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVI68613.1"
                     /translation="VIEGRVISANSTGADRMQTVVNEIGELGGLVKQLATSVANHEDL
                     LATLQANANAPAPVVSAPPQKAEVSAPTIAPQADEKRIMPAALMPVSAEQAPARSHAQ
                     ILGTIRSAIDESRIDIYLQPMVTLPQRKVRFYEAMTRLRDDKEQIVAAEEFVAIAEAS
                     GLIGRIDHMVLLRCVQVLRRLMVRNKEVGVFCNVSSATLANPSTFSQCLDFLEANRAL
                     APALVIEFKQATFRSLGPTETEHLAALAQRGYRFSIDHVTDLRIDPRDLADRGVRFIK
                     VPAALLLDPKQTSSSDIHPSDLSDLLGRFGIDLIAERIEGERAVVDLLDYDVRFGQGF
                     LFAPPRPLRPEGAVNGAPSAAAETTASVRSAVPEPPAVEAQPRASGNAALARRVAGPA
                     "
     gene            1189..>1768
                     /locus_tag="VM910_39495"
     CDS             1189..>1768
                     /locus_tag="VM910_39495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HVI68614.1"
                     /translation="MATLRFVERLRDLVDGVEVVLSDIWGVVHNGLAAFPEACEALQR
                     YREAGGTVVLVTNAPRPADSVQRQLRKLAVPDDTYDAIVSSGDLTRHYVADHAGQKVF
                     WLGPERDSGIYQGLDPALVPLEEADYIVCTGLFDDETETAEDYRTMMSKARERSLPLI
                     CANPDIVVERGDRLIYCAGAIAELYRELGGEVI"
BASE COUNT          293 a          614 c          565 g          296 t
ORIGIN      
        1 gtcatcgaag gccgcgtgat atcggccaat tccaccggcg ccgaccgcat gcagaccgtg
       61 gtcaacgaga tcggcgagct cggcgggctg gtcaagcagc ttgcgacctc ggtcgccaac
      121 catgaagatc tcctggccac gctccaagcc aacgccaacg cgcctgcccc cgtagtgagc
      181 gcgccgcccc aaaaggcgga agtcagcgcc ccgacgattg cgccgcaggc cgacgagaag
      241 cgcatcatgc ccgccgcgct gatgccggtt tctgccgagc aggcacccgc ccgcagccac
      301 gctcagatcc tcggcacgat acgaagcgcg atcgacgaaa gccgcatcga catctacctg
      361 cagcccatgg tgacgctgcc gcagcgcaag gtccgctttt acgaagcgat gacacggctg
      421 cgcgacgaca aagagcagat cgttgccgcg gaggagtttg tcgcgatcgc ggaagcgtcc
      481 ggcctgatcg ggcgcatcga ccacatggtg ttgctgcgtt gcgtgcaggt gctgcggcgg
      541 ctgatggtgc gcaacaagga agtcggcgtg ttctgcaatg tctcgtccgc tacgctcgcc
      601 aatccctcga ccttctcgca atgcctggat ttcctggagg ccaaccgtgc gctggctccc
      661 gccctcgtga tcgagttcaa gcaggcgacc ttccgcagtc tcggcccgac cgaaaccgag
      721 catctcgcgg cgctggcgca acgcggctac cgtttctcga tcgatcacgt caccgacttg
      781 cggatcgatc cgcgagacct tgccgaccgc ggcgtccgct tcatcaaggt gccggcggcg
      841 ctgctgcttg atccgaagca gacctcgtcc tccgacattc atccgtccga cctgtcggac
      901 ctgctcggcc gtttcggcat cgacctgatt gccgagcgta tcgagggcga gcgcgcggtg
      961 gtcgacctgc tcgattacga cgtgcggttc ggccagggat tcctgtttgc cccgccgcgc
     1021 ccgctgcggc ccgagggcgc cgtcaacggg gcgccatccg ccgcggcaga gacgaccgcc
     1081 tccgtcagat cggccgtgcc cgaacctccc gcggtcgagg cacagccgcg cgcgtcaggc
     1141 aatgcggcgc tggcccgccg cgtcgccggc cctgcctgag cgggggccat ggccacgctg
     1201 cgctttgtcg agcggctgcg cgatctcgtg gatggcgtcg aggtcgtgct gtccgacatt
     1261 tggggcgtcg ttcataacgg ccttgcggcc ttccccgaag cgtgcgaggc gctgcagcgc
     1321 tatcgcgaag cgggcggcac ggtcgttctc gtcaccaacg caccgcgccc ggccgattcg
     1381 gtgcagcgcc aactgcgcaa actcgccgtg cccgacgata cctatgacgc gatcgtctct
     1441 tcgggcgatt tgacccgaca ctatgtcgcc gaccatgccg gccaaaaagt attctggctc
     1501 ggacccgagc gcgacagcgg gatctatcag gggctcgacc ctgcgctcgt gccgctggag
     1561 gaagccgatt acatcgtgtg cacggggctc ttcgacgacg agaccgaaac cgccgaagac
     1621 tatcggacga tgatgtcgaa agcgcgcgag cggagcctgc cgctgatctg cgccaacccc
     1681 gacatcgtgg tcgagcgcgg cgaccggctg atttattgcg ccggcgccat cgccgagctc
     1741 tatcgcgagc tcggcggcga ggtgatct
//
LOCUS       DATLJT010001740         5382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1347219,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001740 DATLJT010000000
VERSION     DATLJT010001740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347219"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..798)
                     /locus_tag="VM910_39500"
     CDS             complement(115..798)
                     /locus_tag="VM910_39500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68615.1"
                     /translation="MSEALKQQRQEQGAIVPLAKTSVAAQLNTGLNYLSKYASTGLPL
                     RFNKEGEFIYPTEGDKALAEGTQLAVIWPQARGGFQKFGEKGERPEYRVAPIFGSKPP
                     EREELPDRDPSQWPISKLTAEPEDPWREVQMVPLQSIEDGTVYVFSTMSVTGLRAVSN
                     LLAQCARAAAKDGDSYPVIKLRCGGYEHKRFGWVKVPGFERVGSVPKSDITAAVTSIA
                     GDLEHVLAE"
     gene            complement(795..992)
                     /locus_tag="VM910_39505"
     CDS             complement(795..992)
                     /locus_tag="VM910_39505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68616.1"
                     /translation="MKQSDLPLVRPPDAKLSDAALRIIAEVARLFPGSERDIIIVRQK
                     PIPMNTARIEVGNSQRGKGTT"
     gene            complement(1648..1935)
                     /locus_tag="VM910_39510"
     CDS             complement(1648..1935)
                     /locus_tag="VM910_39510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68617.1"
                     /translation="MMTGNCDRLPVREFNERLTTAKPGQIIVYATGFLAADTAEAFHN
                     KTPTAADLSKVAEAALLASVRGEAFLTQRCIGPRRFEFAHRKPNGVTGKAE"
     gene            complement(3019..4224)
                     /locus_tag="VM910_39515"
     CDS             complement(3019..4224)
                     /locus_tag="VM910_39515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HVI68618.1"
                     /translation="MKRLPTVRELDRLTERGRYACGHGLYLQISEWNTRAWIFRYRHG
                     GRARHVGLGSCLYKSLKEARDEAIEYRRMLARGVDPLDTKRGKVLEDRRAEARKKTFE
                     QAAREYIAAHENTWRGDGSRKQWTQSLEKHAFAKIGAIPVADITVTDVLSVLDPILRE
                     IPETAERVRHRIAKVLDWAIARDLRQNDNPARRPNLLPKRKRTRQHFTAMPYVAVPAF
                     IHELRQRPTIVARALELLILTAARPGELLGMRWSEIDLASAMWTMPGERMKGGRAHRV
                     PLSGRAVELLAGLPRDGSDNAFLGRSAGVRSNPHGLRRLLKRMEHGVTAHGFRASFRT
                     WAGERTNFPRELIEVSLAHAVGDATEEAYNRGDMLARRRQLMEAWSKYCSKPHAKGDV
                     VPLRQGAPA"
     gene            4643..4891
                     /locus_tag="VM910_39520"
     CDS             4643..4891
                     /locus_tag="VM910_39520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HVI68619.1"
                     /translation="MAERVVPHFHNTPGVSVIEIGAREFMCEGELAPFDHPHVYLDMG
                     DASEIICPYCSTLFRYDSTLGSNAARPAECALADGATA"
     gene            4924..>5382
                     /locus_tag="VM910_39525"
     CDS             4924..>5382
                     /locus_tag="VM910_39525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVI68620.1"
                     /translation="MASSRTVIIAGAGIGGLTAALAIARRGFDVTIFDQAQRFEEIGA
                     GIQLSPNASRILIALGLGEPLQRHVVAPQELRVMNARTARVLARAPLGAVADKRYGAP
                     YWVIHRGDLQAVLLDAVRAHPDIVLHLGTRVEDFALDEDGVTIAARSPQRR"
BASE COUNT          903 a         1720 c         1568 g         1191 t
ORIGIN      
        1 tgcgatcacc cgcatgcgaa tgtccgcaga atatgccttc ggcatggctc ggcccccagg
       61 cgaatcaatg ccgccagact cgcccattta caggtccttg ggaatcctgc gattctattc
      121 ggcaagaaca tgctctaggt cgccggcgat tgatgttacc gccgccgtga tgtctgactt
      181 cggcacgctg cccacacgct cgaagccggg caccttcacc caaccgaagc gcttgtgttc
      241 gtagccgccg cacctcagct tgatgacggg atagctgtcg ccgtccttcg ccgccgcgcg
      301 cgcgcattgc gcaagaaggt tcgacaccgc ccgcaggccc gtgacggaca tggtgctaaa
      361 gacgtacacc gttccgtctt cgattgactg cagcggcacc atttgcacct ctcgccaagg
      421 gtcttcgggt tcggcggtga gcttgctgat gggccactgg ctcggatcac gatctggcag
      481 ttcctcacgt tccggtggct tgctgccgaa aatcggggct acccgatatt cggggcgttc
      541 gcccttctct ccgaactttt ggaagccgcc gcgggcctgg ggccagatga cggcaagctg
      601 ggttccctcc gccagcgctt tgtctccctc cgttgggtag atgaattccc cctccttatt
      661 gaagcgaagt ggaagtccgg tggaggcgta cttcgacagg tagttcaatc cggtgttgag
      721 ttgcgccgcg acgctagtct ttgccaacgg cacgattgcg ccttgttctt gtctttgctg
      781 tttcaatgct tcactcatgt ggttcccttc ccgcgctgtg aattgcctac ttcgatgcgc
      841 gcggtattca tcggaatagg tttctgtcgc actatgatga tgtcgcgctc gctgcccggg
      901 aatagtcgcg ccacctcagc gatgatgcgc agcgcggcgt ctgaaagttt ggcgtcgggc
      961 ggccgcacga gcggcaagtc gctctgcttc atgtttcctc ctggccgccg ccgaggagct
     1021 ggcccacggc gccagcggcg gcctacgagc gccgcgctta ggcccccatc tgtttgagaa
     1081 cgtggtccca gatgccgcgc ttaatcccgc gtcggtgatc gtcgcctgct aggcgcttaa
     1141 tgatgtgccg atacacggcc tctaggctgc cgcgcttttt gattccggac ttcaaatcct
     1201 ggcgtgtgaa tgacgtggtt gtggggggca aataggattt tggttttctc ctgccgtaaa
     1261 gctggcgtag cactttctta gacatgactt cccctctgtt gtgcagcatc ggctgccgtg
     1321 cggttaggcc ggggtttcat cccagatttt tcgaacctct tcctccgtga gcgtgtctcc
     1381 tacgagcgcg tctgcgtctc ccgcctcccc taggcgccga caaaaactgg caacgcgaac
     1441 gccgcgcccg ttgctcgtcg ctccacggca gtctctcgat gattgccatt tgctcttcgc
     1501 tgaacagtct gagatcgggg agctttcccc cgtgcgccag ataattatca atgtcgtaga
     1561 tgccccaagc tgctccgcgc cccgccatgt ctaaccctgc catttctcac ttccctcttg
     1621 ttgctgcggc atcggctgcc gcgcgcctta ttcggctttg ccggtaactc cattaggttt
     1681 tctgtgcgcg aactcgaacc gccttggtcc aatgcaccgc tgtgtcaaaa acgcctcgcc
     1741 cctaaccgag gccagcaacg cggcctcggc caccttgctt aggtccgccg ccgtcggcgt
     1801 cttgttgtga aatgcctcgg cggtgtcggc tgcaagaaag ccggttgcat agacgatgat
     1861 ttggcctggc tttgccgtcg tcagccgctc gttaaattct cttaccggga gacggtcgca
     1921 gttcccggtc atcatcgcgc cctcgatttc agcttcgaaa tgacgggggc ggcgacatcg
     1981 cgaagctgtt gccagctatc atcagcgggg cgccattcac ggccggcctt gcgggcctca
     2041 acccactcga tgagttgatt gaacgaccac gcgattgtgt ttgcggtcag ccgaaaaggc
     2101 ttcggcgccc tccccgcgtt ctgtagctcg cgccaggtag agcaaggaac gccagtgatg
     2161 tgttctcgct ctggctcgcg cacggcgcga tctggtcggt tgtcgttcat tgcaatcgcg
     2221 ttcaagggtg aggtcgccag cgtcctggcg acctcgttgc cttgccttgg cttgttgctg
     2281 ttggaccgcg tcggcgatgg cgcttacgcg cagaggtgat ctgggtccgt caggtcaggt
     2341 tcgtctgctg acgacgacct tgtctcacag cctgattggg gaatggaggg ggttattccc
     2401 ccttaggagc tcgaggtgtg attttcagtg cggcttccaa ctggcgctcc caagtctccc
     2461 gcgacagcag ctcgcttacg aaagcttttt ccctaaatag ccgcgcagcg tccctctgga
     2521 gttcttcagc gctcgccccc tcgagccacg ccatctcctc tttgtaccat tccatagctt
     2581 ggcggttttt taccttaccc tttttaaatt cccgccgcca cgacaaggtt tctttgttcg
     2641 acaccccgtc aagcttgtag ccgcgaatta cttgtggcgc ggcagcatca tacgttatgc
     2701 ccgttgcatg aagcagctcg atagcttaat atatgccgcc agctctcccg tttcggcgcg
     2761 ccccaaatac gcctcgcgaa atctgatcag cgcatcctgc aatatcagcg gcctatgaat
     2821 tttccaccac tctttaagat attcttcgtg ggcggctgtg gtgcctggcg gcatacgcgc
     2881 cctccaccat tcttctaaag atcgttcccg gttgtttgtc atggcgcgcc cattggtgcg
     2941 gggcttctct taaccgatct gccgcagtgc caccacatca gcttcgacgc gcgggccggc
     3001 cacaatattc gctccaagct atgccggcgc tccttgacgc agcggcacca cgtcgccttt
     3061 ggcgtgtggc ttgctgcaat atttgctcca cgcttccatc aactgccgtc gccgcgccag
     3121 catatcgccg cggttatagg cttcctccgt cgcatcaccg acggcgtgcg caaggctgac
     3181 ctcgattagc tcgcgtggga aattggtgcg ttccccagcc cacgttcgga aagatgcgcg
     3241 aaagccgtgc gccgtcacgc catgctccat gcgcttcagc aaccgccgca ggccatgcgg
     3301 attcgacctg acgcccgcgg aacggccaag gaatgcgtta tcgctgccgt ctcgcggcaa
     3361 gccggccaac agctcaaccg cgcggccgga cagcggcacg cgatgcgccc gcccgccttt
     3421 catgcgctct cccggcatcg tccacatcgc ggacgccagg tcgatttcgg accaacgcat
     3481 gcccagcaac tcaccggggc gcgccgcggt taggatcaga agttcgagcg cccgcgcgac
     3541 tatggtcggg cgctgtcgca actcgtgtat gaacgccggc accgcgacat aaggcatagc
     3601 ggtgaaatgc tgccttgtgc gcttccgttt tggcaacaaa ttcggccgcc tggccggatt
     3661 gtcgttctgc cgtaagtcgc gcgcaatggc ccaatccaaa accttggcga tgcgatgccg
     3721 cacgcgctca gcagtttcgg gaatttctcg caaaatcgga tcaagaacgc tcaacacatc
     3781 tgtgacagtg atgtcggcca ccgggatggc gccaatcttg gcgaaggcgt gcttttcgag
     3841 gctttgcgtc cactgtttgc ggcttccatc gccgcgccag gtgttttcgt gcgccgcgat
     3901 gtattcgcgc gcggcctgct caaacgtctt cttgcgggct tcggcgcgcc ggtcttcaag
     3961 cactttgccg cgcttcgtgt cgagcggatc gacgccgcgg gcaagcatac gccgatattc
     4021 aatcgcttcg tcgcgcgcct ctttcagcga tttatagaga caggagccaa ggccgacatg
     4081 acgcgcgcga ccgccgtggc gatatcgaaa aatccatgcg cgtgtattcc attcagaaat
     4141 ttgcaagtag agcccgtggc cacaagcata gcggccacgc tcggtgaggc ggtcaagctc
     4201 gcgcactgtg ggaagccgtt tcacggtcga ctccttaagc agcgtttttt ctccgtgaca
     4261 cgcctaacct cccatctgcc taccattcga ccctaccatg ccccgcactg cctcggaagt
     4321 gctcagaaga caaaaatgtg aaaagtgcct atggaacttg gctaacctgc aatgcctcgg
     4381 aacgcctcgg aagccagccg actggaatcg ttctcctgcg cccccgcccc caatttcgcc
     4441 aagcgcagtg gcgccggcaa gagaccacgg cgaaaaattg ccgattagcg actgctgggg
     4501 acgtgcaccc cgccgcggca tttggattgc ggtcggttgg gggacgagaa cgattggcct
     4561 agccatggcc tacccggctg gctatggtgc cgcgcggtcg cgggaagcgt aagctcggtc
     4621 acgtcgaagg cggacgaaga acatggcgga gcgggttgtt cctcacttcc acaacacgcc
     4681 gggcgtctca gtcatcgaga tcggcgcgcg ggagttcatg tgcgagggcg agttggctcc
     4741 gttcgatcac ccgcacgtct acctcgacat gggcgatgca agcgagatca tctgtcccta
     4801 ttgctccacg ctctttcgtt acgattccac gcttgggtcc aatgcggcac gcccggcgga
     4861 atgcgcgctg gcggatggag cgaccgcgta gcagcagcaa tcgatgcgac ggagacatga
     4921 gccgtggcgt cctcgcgcac cgtcatcatc gctggcgcgg ggatcggggg gctgaccgcg
     4981 gctttggcga ttgcgcggcg cggattcgac gtcaccatat tcgaccaggc gcaacgcttt
     5041 gaagagatcg gcgcgggcat tcaactgtcg cccaatgcgt cgcgcattct gattgcgctc
     5101 ggcctcggcg agccgttgca gcggcatgtc gtcgctccac aggagctgcg ggtcatgaac
     5161 gcgcggacgg cgcgggtgct tgcgcgcgcg ccgctcggtg cggtggccga taagcgctac
     5221 ggcgcgccct actgggtgat ccatcgcggc gacctgcagg cggtgctgct cgacgcggtg
     5281 cgcgcccatc ccgacatcgt ccttcatctc gggacgcggg tcgaagattt cgcgctcgac
     5341 gaagacgggg ttacgatcgc cgcccgctcc ccgcagcgtc gg
//
LOCUS       DATLJT010001741         1832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1575170,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001741 DATLJT010000000
VERSION     DATLJT010001741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575170"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VM910_39530"
     CDS             complement(<1..639)
                     /locus_tag="VM910_39530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HVI68621.1"
                     /translation="MAVMRSGASLTRRRFLTTAASSAVMAAAGGIAKPALSRAADRPV
                     ISHGVQSGDVCVDSAIVWARADRPSRMLVEIATSESFKTIRSAVFIDALPETDFTAKA
                     LLEGLPAGQDVFYRICFQDHSFPSALGSPQVGRFRTAPSERRTISFVWSGDTAGGGWG
                     IDEARGGMRTYATMLKSRPDFFIHCGDHIYADCPLRAEHKLPNGEVWRNIVTE"
     gene            970..1407
                     /locus_tag="VM910_39535"
     CDS             970..1407
                     /locus_tag="VM910_39535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68622.1"
                     /translation="MDDKYFGSHELHGREHIVQGSSDRGFGYVFAGFAALVAALSLYK
                     GGTHWPYWLAAAVMFALVAFYRPSLLAPLNRLWTKLGLVLFAVVSPLALGIVYYGCIT
                     PVGWLMRPSGKDPLRLRFEPERKSYWVSRQPPGPPPDSLMKQF"
     gene            1418..1567
                     /locus_tag="VM910_39540"
     CDS             1418..1567
                     /locus_tag="VM910_39540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HVI68623.1"
                     /translation="MSFLREFAAFLLERKKYWLLPVFIVMAIFGGLIVLTKGTAVAPF
                     IYTLF"
     gene            1574..>1832
                     /locus_tag="VM910_39545"
     CDS             1574..>1832
                     /locus_tag="VM910_39545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HVI68624.1"
                     /translation="MRVLGISAFYHDSAAALIADGRIVAAAQEERFTRKKFDANYPRH
                     AIEYCLNAGGVTLADVDYVAFYDKPFLKFERLLETYLTFSPR"
BASE COUNT          325 a          587 c          566 g          354 t
ORIGIN      
        1 ctcagtaacg atgttcctcc acacctcgcc atttggaagc ttgtgctccg cgcgcagcgg
       61 gcagtcggcg tagatgtggt cgccgcaatg aatgaaaaaa tcgggccgcg atttcagcat
      121 ggtcgcgtaa gtgcgcatgc ccccgcgcgc ctcgtcgatg ccccagccgc caccggcggt
      181 atcgcccgac cacacgaacg agatggtgcg ccgttcgctc ggcgccgtgc gaaaacgccc
      241 gacctgcggg gaaccgagcg cgctcggaaa cgaatgatcc tggaagcaga tgcgatagaa
      301 cacgtcctgc ccggcgggca gaccctcgag caacgccttg gcggtgaaat cggtctccgg
      361 cagagcgtcg atgaagaccg cgctgcgaat agtcttgaag ctctcgctgg tcgcgatctc
      421 gaccagcatg cgcgatggtc tatccgcccg cgcccagaca attgcggaat cgacgcagac
      481 atcgccggac tggacgccgt ggctaatgac cggccgatcg gcggcgcggc tcaatgcggg
      541 cttggcgatg ccgccggcgg ccgccatcac cgccgacgaa gccgcagtgg tcaagaagcg
      601 gcgccgcgtc agcgacgcgc cgctgcgcat gacggccatc gggcctccgc cgtcgaatca
      661 ccgggacgga agctgcccgc gtcgtctgac agggactcga cacttgtgcg acagctgcgt
      721 gaagacctcg cagcacgccg agctgcagag gctcaaccga atgcggatcc ataacgctag
      781 atacagaggt ggacatcagc gcgagctctc cgcatccacc gtcgtcaagc ggcggagagc
      841 ggtggcatgt tcccgaggtg ttctgctgcg aacttcgctt gcctctggga accggaccag
      901 tatgttgcaa tcgcattctg aatatgcggt ttcatgcgcg gggggcgcat cagcacgggg
      961 aacaacggcg tggacgacaa gtactttggc tcccatgagc ttcacggccg ggagcatatc
     1021 gtgcagggct cgtcggatcg cgggttcggc tacgtctttg ccggctttgc ggcgcttgtt
     1081 gcggccttat cgctctacaa gggcggtacg cattggccct attggctggc ggcggccgtc
     1141 atgttcgcgc tcgtcgcatt ttaccgtccg agtctgctcg ctccgctcaa ccggctgtgg
     1201 accaagctgg gcctcgtcct gttcgcggtc gtgagcccgc tcgccctggg gatcgtatat
     1261 tatggctgca ttacgccggt cggctggctc atgcggccca gcggcaagga ccctttgcgg
     1321 cttcgcttcg agcctgagcg caaaagctat tgggtctcgc gacagcctcc aggtccgccg
     1381 cccgacagcc tcatgaagca attctgagta cccgatgatc tcctttctac gtgaattcgc
     1441 cgcgttcttg ctggagcgta agaaatactg gctgctgccg gtgttcatcg tcatggccat
     1501 attcggcgga cttatcgtgc tcacgaaggg cacggcggtt gcgccgttca tctacacgct
     1561 attctgacgg gccatgcgag tcctcgggat ttctgcgttc tatcacgaca gcgcggccgc
     1621 gttgatcgcc gacgggcgca tcgtggccgc cgcgcaggaa gaacgtttca cccgcaagaa
     1681 gttcgatgcc aactatccgc gccacgcgat cgaatattgc ctcaacgccg gcggcgttac
     1741 gctggcggat gtcgactacg tcgccttcta tgacaagccc tttctgaagt tcgagcgcct
     1801 gctcgaaacc tatctcacct tcagcccccg cg
//
LOCUS       DATLJT010001742         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3875843,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001742 DATLJT010000000
VERSION     DATLJT010001742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875843"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1184
                     /locus_tag="VM910_39550"
     CDS             87..1184
                     /locus_tag="VM910_39550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVI68625.1"
                     /translation="MSALPDQSPSRRRLLLASDRSDRSSELANILRSVGDVDTVATKE
                     IPPFPNGRFSGVVVDINLRSPESVQTVRNRLRADVYSDMPRLFVLAEALHHATMQAWA
                     LGATDTISRPFNADAILQRIHAAFPDSAGFDATDRGKTLNRGVAAAHEVMVKIFERLP
                     AGVPLTLDDVVQSEIKIIKAIKHSSLREWLTVIGCHHTDSYRHCLFVTGFAVAFAQHL
                     GMREDDQRRLTRAALLHDVGKAFVPVAILDKKGALTEEELAVVRKHPQRGYDALAAQG
                     GFPPEMLDVVLHHHELLDGSGYPHGLRADQISDIVRLTTIVDIYAALVEKRAYRVPFT
                     HSKAFAVMEGMGEKLDQQLLQVFRPVAFGCY"
     gene            complement(1237..1647)
                     /locus_tag="VM910_39555"
     CDS             complement(1237..1647)
                     /locus_tag="VM910_39555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVI68626.1"
                     /translation="MPVKVNAIDHIVINVSDVARSAGWYAKILGMEIRVFDPGDGKSP
                     RTSLVFGSQKINVRPKHADKVEWFTADHETAGSDDLCFLTSSTPEEVVAHLEANGVAI
                     EEGPTRKQGARGTLLSVYCRDPDGSLIEISSYEK"
     gene            1771..>2173
                     /locus_tag="VM910_39560"
     CDS             1771..>2173
                     /locus_tag="VM910_39560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68627.1"
                     /translation="MFSRRAAIGLMATGMSSLLVSARRAAAADYPTRPIRFLVAYSPG
                     GATDIIARLIGQRLSERLGQQFVIENKPGAGNNIGTEAAVNAEPDGYTVLLVNPANYI
                     NTSLYANLKFNFVNDIAPVASFNRVPNVMTVN"
BASE COUNT          383 a          714 c          665 g          411 t
ORIGIN      
        1 ggacatgttg atcatgccta ttaagccgcg gggaacgcat cgtcgggtag catgatccgg
       61 tattgcctct gcgagaccca aagcccatgt ccgctctccc cgatcagtct ccatcccgcc
      121 gccggctgct gctcgcgtcc gaccggagcg accgctccag cgaactcgcc aacatcctgc
      181 gctccgtcgg cgatgtcgat acggtggcga cgaaggagat cccgccgttc ccgaacggca
      241 ggttttcggg cgtcgtggtc gatatcaacc tgcgctcgcc ggagagcgtg cagacggtcc
      301 gcaaccgctt gcgggcggac gtctacagcg acatgccgcg gctgttcgtg ctggcggagg
      361 cgctgcacca cgccaccatg caggcgtggg cactcggcgc gaccgatacc atttcgcggc
      421 cgttcaatgc cgatgccatc ctgcagcgca tccacgccgc attcccggat tccgcaggct
      481 ttgacgcgac cgaccgcggc aagaccttga accggggcgt tgcggccgcg cacgaggtga
      541 tggtcaagat ctttgagcgg ctgccggcag gcgttccctt gaccctcgac gatgtggtgc
      601 agtcggaaat caagatcatc aaggcgatca agcattcctc cttgcgcgaa tggttgaccg
      661 tgatcggctg tcaccacacc gacagctatc gccattgtct gttcgttacg ggcttcgcgg
      721 tggctttcgc gcagcatctc gggatgcgcg aggacgacca gcggcggctg acccgcgcgg
      781 cgctgctgca cgatgtcggc aaggcattcg tgccggtggc gatcctcgac aagaagggcg
      841 cgctgaccga ggaagagctt gccgtggtcc gcaagcaccc gcagcgcggc tacgacgcgc
      901 ttgcggcgca aggcggcttt ccaccggaaa tgctggacgt cgtgctgcac caccacgaac
      961 tgctcgacgg cagcggctat ccgcatggcc tgcgcgccga ccagatcagc gacatcgtgc
     1021 ggctgacgac catcgtcgac atctatgccg cgctggtgga gaagcgcgcc tatcgcgtgc
     1081 cgttcaccca ctccaaggcg tttgccgtca tggaagggat gggcgagaag ctcgaccagc
     1141 agttgctgca ggtattccgt ccggtggcgt tcggctgcta ctgaggctca atccgctgcg
     1201 tcgccggccg cttctcgcag tgacggggcg agacgctcac ttttcatacg acgaaatctc
     1261 gatcaggctg ccgtcggggt cgcggcaata gaccgacagc agagtgccgc gcgcgccctg
     1321 tttcctggtc gggccttcct cgattgcaac gccgtttgcc tcgagatggg cgacgacctc
     1381 ctccggcgtg ctcgacgtca gaaagcacaa atcgtcgctg ccggcggtct cgtggtccgc
     1441 ggtgaaccat tcgaccttgt cggcatgttt cggccggaca ttgattttct ggctgccaaa
     1501 tacgagcgag gtgcgcgggc tcttgccgtc gccggggtcg aataccctga tctccatgcc
     1561 gaggattttt gcgtaccacc cagccgagcg cgcgacatcg ctgacattga tcacgatatg
     1621 atcgatcgca ttgaccttga ccggcatggc tgatccctgt cctctcgcat ggcccgcgcg
     1681 cgtggcgcgc tatttgcgct ccacgccttg cttgttacaa cgtgcgtcca tatcagttca
     1741 agaacaacga aaagcgggga gatatccgac atgttttcac gccgcgccgc gatcggtctc
     1801 atggcaaccg gcatgtccag cctgctcgtg tcggcgcgcc gcgccgcggc ggcggactat
     1861 ccgacacggc cgatccgctt cctggtcgca tattcgccgg gaggtgcgac cgatatcatc
     1921 gcccgcctga tcgggcagcg gctctccgaa cggctcggcc agcaattcgt gatcgagaac
     1981 aagccgggcg ccggcaacaa tatcggcacc gaggctgcgg tgaacgccga gcccgacggc
     2041 tatacagtgc tgctggtgaa cccggcgaac tacatcaaca cctcgctcta cgccaacctc
     2101 aagttcaatt tcgtcaacga catcgcgccc gtggcatcgt tcaatcgcgt gcccaacgtg
     2161 atgacggtca aca
//
LOCUS       DATLJT010001743         6385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2728006,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001743 DATLJT010000000
VERSION     DATLJT010001743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728006"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VM910_39565"
     CDS             <1..376
                     /locus_tag="VM910_39565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI68628.1"
                     /translation="AIIGIILLIGIVKKNAIMMIDFALDAERNEGKSPREAIYQACLL
                     RFRPIIMTTMAAVLGALPLMVGTGAGSELRHPLGISIVGGLLLSQLLTLFTTPVIYLW
                     FDRLAIRFSGGAEGANQPSGTG"
     gene            380..3664
                     /locus_tag="VM910_39570"
     CDS             380..3664
                     /locus_tag="VM910_39570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVI68629.1"
                     /translation="MNPSSPFIRRPVATTLLTFGLAAAGAVAFFKLPVSPLPQVDFPT
                     ISVQATLPGASPEDVATTVASPLERHLGQIADVTEMTSSSSVGQARITLQFGLNRNID
                     GAARDVQAAINAARADLPTSLRTNPTYRKVNPADAPILVLTLTSDTATRGEVYDAAST
                     VLAQKLSQVEGIGEVGVSGSSLPAVRVDLIPQALYKYGIGLEDVRAALSSANAHSPKG
                     GIDVGDQRYQIYANDQANKAEDYKHLIIAYRNGAAVHLSDVGEVIDGVENLRNAGLSN
                     GKPAVLIILYRQPGANIISTVDAVKALMPVLRASISPAIDIGLAVDRSTTIRTSLRDV
                     ERTLVLAVLLVIIVVFAFLRNLRATLIPVVAVSVSLVATFGAMYLVGYSLDNLSLMAL
                     TVATGFVVDDAIVVLENVTRYIEEGMSPLNAALKGANEVGFTVLSMSISLIAVFIPIL
                     LMSGIIGRLFREFAMVISISIMISLAISLATTPMMCAVLLRQETGSHGRLYRVSERFF
                     EAMLGFYRSTLTSALEHPFSIVLIFFAAIGLNVYLYVVIPKGFFPQQDTGRIIGSIQA
                     DQAISFQLMQQKLSQFVSIIKKDPAVETVVGFTGGAQTNSGFLFVSLVPLGERHISAD
                     AVIARLRREMAVVPGATLFLQAVQDIRAGGRASNAQYQYTLQGPTFEELNEWAPKIAA
                     ALQHEPDLADVNSDQQNRGLESDVTIDRDAAARLGVTVSQIDNTLYDAFGQRQVSTIY
                     VARNQYHVIMEVAPRYWQNPETLKDVYVSTSGASVGGSQSTNAVAGTVVSSSTSTALA
                     AANAQAARNLATNSIGQTGKGVASTGSAVSTKQETMIPLSAVAHFTRGATPLAVNHQG
                     LLVATTISFNLPPNVSLSTAIATIDATMNKLGVPATIRGSYQGTAKVFQDSLANQPFL
                     ILAALVTIYIVLGVLYESYIHPLTILSTLPSAGVGALLALMAFKTEFSIMALIGVILL
                     IGIVKKNAIMMIDFALEAERKRGLSSLEAIREACLLRFRPIMMTTMAAMLGALPLALG
                     MGDGGELRQPLGISIVGGLILSQALTLYSTPVIYLYLDRFRLWSQRFRGDSAPHTPGP
                     SLQPGE"
     gene            3665..5176
                     /locus_tag="VM910_39575"
     CDS             3665..5176
                     /locus_tag="VM910_39575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVI68630.1"
                     /translation="MAGAGPLPFLERIRSRIRIDVARAGARRRSAGALLALAMSASLS
                     GCILGTERPELNLEVPEAYHAARNGNADAAVPALDWWRGFRSAELTALMEAAQLHNLD
                     IAVSIAQIVQADALVGVSGAALLPTLTGNASAERQKQSSANTVASGNGFGGTTFNTFQ
                     TGLTASYMLDFWGKNRAALYAAEENATVSRYNREVVTLTTMVTVANTYFQVLAAQDEL
                     RVTRQNLAAAERILNLVRKQFNGGTASQLDVSQQEALAATQRAAIPPLEVTLRQNIAA
                     LALLVGRAAPNFTVHGGTTRQIVVPRVTPGLPSELLYQRPDVRQAESQLASSSFSVES
                     ARAAFFPQIALTGTTGFQSAALASLFTPGAWFYTLTASLTQPIFDGFLLESQLKQAKG
                     VQLQNLQAYRKAVLSAFADVEKALIALQQFTLQERLQQQVVDASQQAFDVAEKQLTGG
                     TINLITVLQAEQTLFTAQNNLVLVRLSKLQAASSLFQALGGGWTPAGTLASVE"
     gene            5207..5512
                     /locus_tag="VM910_39580"
     CDS             5207..5512
                     /locus_tag="VM910_39580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68631.1"
                     /translation="MLTTIAALLLSAGVSLGAAHAQFVTLTPPNIPLAFGMTPEQASQ
                     ALGVGLYYVRGRPGDELLLALPNVKGAALASRSDGLYLQFRRGRLTGWKGDWGTIRP"
     gene            5509..5901
                     /locus_tag="VM910_39585"
     CDS             5509..5901
                     /locus_tag="VM910_39585"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HVI68632.1"
                     /translation="MTKRKAQRITAHLVGAALIALFGAEASQAQDLGVRPLRYGNTSG
                     WFYDNRNDDRDVPNNGFFPGNYTVDPTTAWLGAAGVIAGNSYRSPLPYPSQVVIGPAP
                     SSVSCMRYRSYDPATGTYVGRDGLRHRC"
     gene            complement(6136..>6385)
                     /locus_tag="VM910_39590"
     CDS             complement(6136..>6385)
                     /locus_tag="VM910_39590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HVI68633.1"
                     /translation="QAGEFVHEEIDKIRRGEHGARSPQQAIAIGLSEARRAGVDLPPP
                     RKGKAKSRTRRSAKYAKAGQGRRKRGDGRAFRAQSNRC"
BASE COUNT         1094 a         2125 c         1984 g         1182 t
ORIGIN      
        1 ggcgatcatc ggcatcattc tcctgatcgg catcgtgaaa aagaacgcga tcatgatgat
       61 cgacttcgcg ctcgacgccg agcgcaacga gggcaagtcg ccgcgcgaag cgatttacca
      121 ggcctgcctg ctgcgcttcc ggccgatcat catgacgacc atggccgcgg tgctcggcgc
      181 gctgccgctg atggtcggca cgggcgcggg ctccgagctg cggcatccgc tcggcatctc
      241 catcgtcggc ggcctcctgc tcagccagtt gctgaccttg ttcacgacgc cggtgatcta
      301 tctctggttc gaccggctcg ccatacgctt ctccggcggc gcggaaggcg cgaaccagcc
      361 gagcgggaca ggttaggtca tgaatccgtc aagccccttc atccgccggc cggtcgccac
      421 gaccctgttg acgttcgggc ttgcggcagc cggcgcggtc gcgttcttca agctgcccgt
      481 ctcgcccttg ccgcaggtcg atttcccgac catttcggtg caggcgaccc tgcccggcgc
      541 aagccccgag gacgtcgcca ccactgtggc aagccccctc gagcgccatc tcgggcagat
      601 cgccgatgtc accgagatga cgtcttcaag ctcggtggga caggcgcgca tcaccctgca
      661 gttcggcttg aaccgcaaca tcgacggggc cgcgcgcgac gtgcaggctg cgatcaacgc
      721 ggcccgcgcc gacctgccga ccagcctcag gaccaatccg acctaccgca aggtcaaccc
      781 ggccgacgcc ccgatcctgg tcctgacgct gacctccgac accgcgaccc gcggcgaagt
      841 ttacgatgca gcatcgaccg ttttggcgca aaagctgtca caagtcgaag gtatcggcga
      901 ggtcggggtc agcggcagct cgttgccggc ggtgcgcgtg gacctcattc cgcaggcgct
      961 ctacaaatac ggcatcggtc tcgaagacgt tcgcgccgcg ctgtcaagcg ccaatgcgca
     1021 cagcccgaag ggcggcatcg atgtgggcga ccagcgctat cagatttacg ccaatgacca
     1081 ggccaacaag gcggaggact acaaacacct catcatcgcc tatcgaaacg gcgcagcagt
     1141 ccatctaagc gacgttggcg aagtcatcga cggggtcgag aacctgcgca atgccgggct
     1201 ttccaatggc aagccggcgg tgctgatcat cctctaccgg cagcccggtg cgaacatcat
     1261 ctcgaccgtc gacgcggtga aggcgctgat gccggtactc cgggcatcga tctcgccagc
     1321 catcgacatc ggccttgccg tcgaccgctc gaccaccatt cgcacctcgc tgcgcgacgt
     1381 cgagcgcacg ctggtgcttg ccgtgctgct cgtgatcatc gtggtattcg cgttcctccg
     1441 aaacctgcgc gcgaccctga tccccgtcgt cgctgtctcg gtctcgctgg ttgcgacttt
     1501 cggtgcgatg tatctggtcg gctacagcct cgacaacctg tcgctgatgg cgctgacggt
     1561 ggcgacgggc ttcgtcgtcg acgacgcgat cgtcgtactg gaaaacgtca cccgctacat
     1621 cgaggaagga atgtcgccgc tgaacgcggc gctcaagggc gcgaacgagg tcggcttcac
     1681 cgtgctctcg atgagcattt cactgatcgc cgtctttatc ccgatcttgc tgatgtccgg
     1741 cattatcggt cgcctgttcc gcgaattcgc gatggtgatc tcgatctcga tcatgatttc
     1801 gcttgcgatt tcgctggcga ccacgccgat gatgtgcgcg gtgctgctgc gccaggaaac
     1861 gggaagccat ggtcggttgt accgcgtgag cgaacgcttt ttcgaggcga tgcttggttt
     1921 ctatcggagc acgctgacga gcgcgctgga gcatcccttc agcatcgtgt tgatcttctt
     1981 cgccgccatc ggcctgaacg tttacctgta tgtcgtgatc ccgaagggtt tcttcccgca
     2041 gcaggacacc ggacggatca tcggctctat ccaggccgac caggccatct cgttccagct
     2101 catgcagcag aagctctcgc agttcgtcag catcatcaag aaggaccccg cggtcgaaac
     2161 cgtcgttggc ttcaccggcg gcgcccagac caattccgga ttcttgttcg tttcgctggt
     2221 cccgctcggt gagcgccata tttcggcaga cgcggtgatt gcacggcttc gacgcgagat
     2281 ggcggtcgtt ccgggtgcga ccctcttcct gcaggcggtg caggatattc gggctggcgg
     2341 ccgcgccagc aacgcgcaat atcaatacac cctgcaggga ccgacattcg aggagctcaa
     2401 cgaatgggcg ccgaagattg cggccgcgct gcagcacgaa cccgatcttg ccgacgtcaa
     2461 cagcgaccag cagaacaggg ggctggaatc cgacgtcacg atcgatcgcg acgcggctgc
     2521 gcggctcggc gtcaccgtga gccagataga caacacgctc tacgacgcgt tcggccagcg
     2581 ccaggtctcg accatctatg tcgcgcgcaa ccagtatcat gtcatcatgg aggtggcccc
     2641 gcgatactgg cagaatccgg agacgctgaa agacgtctat gtctcgacct ccggcgcctc
     2701 ggtcggcgga tcgcaatcga ccaatgcagt ggcaggcacc gtggtctcgt cgagcacgtc
     2761 gaccgccctg gccgccgcga acgcgcaggc cgcgcgcaac ctcgcgacca actcgatcgg
     2821 ccagaccggc aagggcgtgg cgtcgacggg ctcggccgtc tcgaccaagc aggagacgat
     2881 gatcccgctg tcggcggtgg cccatttcac gcggggcgcg accccgcttg cagtgaacca
     2941 tcaggggctc ctggtggcga ccacgatctc cttcaacctg ccgcccaacg tctcgctgag
     3001 cacggcgatc gccaccatcg acgccaccat gaacaagctc ggcgtgccgg cgacgatccg
     3061 cggctcttat cagggtacgg cgaaggtgtt tcaggattcg ctggccaacc agcccttcct
     3121 cattctcgcg gcgctggtca cgatctacat cgtgctcggc gtgctttacg agagttacat
     3181 tcacccactc accatcctgt cgacgctgcc ctccgcaggt gtcggcgcgc tgctggcgct
     3241 gatggcgttc aagaccgagt tcagcatcat ggcgctgatc ggcgtcattc tcctgatcgg
     3301 catcgtgaag aagaacgcga tcatgatgat cgattttgcg cttgaagcag agcgcaagcg
     3361 ggggctcagt tcgctcgaag cgatccgcga agcctgcctg ctccgcttcc ggccgatcat
     3421 gatgacgacc atggcggcga tgctcggggc ccttccgctc gccctcggca tgggcgacgg
     3481 cggggagctt cgccagccgc tcggcatctc cattgtcgga ggcttgattc tgagccaggc
     3541 gctgacgctt tattccacac ctgtgatcta tttgtatctg gacagatttc gtctctggag
     3601 ccaacgtttt aggggcgact cagcacctca tacgcccggg ccttctctgc aaccgggcga
     3661 atagatggca ggcgccggtc cactcccgtt tctcgagcga atccggagcc gtatccggat
     3721 cgatgttgcg cgcgccggcg cccggcgccg ctcggctggc gcactgcttg cgcttgcgat
     3781 gagcgcgtcg ctctcgggct gcatcctcgg caccgaacgc cccgaactca atcttgaagt
     3841 gcccgaggcc tatcacgcgg cacgcaatgg caatgcggac gccgccgttc cggccttgga
     3901 ctggtggcgc ggcttccgtt ccgccgagct caccgcgttg atggaggcgg cccagcttca
     3961 caacctcgat atcgccgtgt ccatcgccca gatcgtccag gccgatgccc tggtcggggt
     4021 gtcgggcgcg gccctgctcc cgacgctgac gggaaatgcg agcgccgaac gacagaagca
     4081 gagcagcgcc aataccgtcg ccagcggcaa cggcttcggc gggacgacct tcaatacttt
     4141 ccagaccgga ttgaccgcga gctacatgct cgacttctgg ggcaagaacc gcgcggcgct
     4201 ctatgcggcg gaggaaaatg cgacggtctc gagatataat cgcgaggttg tcacactgac
     4261 gacgatggtc accgtcgcca atacctattt ccaggttctc gccgcgcagg acgagttgcg
     4321 cgtcacccgc cagaacctgg cagcggcgga gcgcatcctc aatctcgtca gaaagcagtt
     4381 caacggcggt acggcctcgc agctcgacgt gtcgcagcag gaggcgctgg ccgcgaccca
     4441 gcgcgccgca attcccccgc tcgaggtcac gctcaggcaa aacatcgctg cacttgcgct
     4501 tctggtcggt cgcgcagcgc caaacttcac cgttcacggc ggcacgacgc gccagatcgt
     4561 ggtgccgcgg gtcactccgg gcctgccgtc ggaattgctt taccagaggc cggacgtccg
     4621 ccaggccgag tcgcagctcg cctcctcgag tttcagcgtc gagtccgcgc gcgctgcgtt
     4681 ttttccgcag atcgcgctca cgggcacgac cggatttcag agcgcggcgc ttgcgtcgct
     4741 gttcacgccg ggcgcctggt tctacacgct gaccgcaagc ctgacccagc cgatcttcga
     4801 cggcttcctg ctcgagagcc agttgaagca ggccaagggc gtgcaactgc agaacctgca
     4861 ggcctatcgc aaagcggtgc tgtccgcctt tgccgacgtc gagaaggcgc tcatcgcctt
     4921 gcagcaattc acgttgcagg aacgcctgca gcagcaggtc gtcgacgcct cgcagcaggc
     4981 tttcgacgtc gcggaaaagc agttgaccgg cggcaccatc aatctgatta ccgtgctgca
     5041 ggcggagcag acgctgttta cggcgcaaaa caacctggtg ctggtccgcc tgtcgaagct
     5101 tcaagccgca agcagcctgt tccaggcgct cggcggcggc tggaccccgg ccgggacgct
     5161 ggcatcggtg gagtgaggga ggatgcggaa agcgagcaga aagcccgtgc tgacaacgat
     5221 agcggccttg ctgctgtcgg ccggcgtctc gctgggcgca gcgcatgccc agttcgtcac
     5281 gttgacgccg ccgaacatcc cgcttgcgtt cggcatgact cccgaacagg cctcgcaggc
     5341 gcttggcgtt ggactatact atgtgcgcgg cagacccggc gacgagcttt tgcttgcgct
     5401 gccgaacgtc aagggcgcgg cgctggcgag ccgcagcgac gggctctatc tgcagtttcg
     5461 gcgcggaagg ctgacgggat ggaaaggcga ttgggggacg atcaggccgt gacgaaacgg
     5521 aaagcccaaa ggatcacggc acacctcgtc ggcgccgccc tgatcgcgct gttcggcgcg
     5581 gaggcgtcgc aagcgcaaga cctcggcgtt cgtcccctcc gctacggcaa caccagcggc
     5641 tggttctacg ataatcgcaa cgacgatcgc gacgttccca acaatggatt cttcccgggg
     5701 aattacaccg tcgatccgac aactgcctgg ctcggtgcgg caggcgtcat cgcaggtaat
     5761 tcctaccgct cgcccttgcc ctatccgtca caggtcgtga tcggacccgc gccctcttcc
     5821 gtcagttgca tgcgctaccg gtcatacgat ccggctaccg gcacctatgt cggacgggac
     5881 ggactgcgtc atcgctgctg aatcgccggc gagatcgaga acagggcgcg ccgaaccgac
     5941 gcgcaaatca ggcacgtttc cgacgcgctc tcgtacgtgc ggccttcttg gcggcagccg
     6001 agcgagcctt ggcacctttg gtccgtacgg ccttgcgggc ggcggctgaa cgcgaggcgg
     6061 cgctgcgtcg gctggctgcg cgcttggctt gttgcgacag cgcacgtcgt gaggcggagc
     6121 tccgcggctc gtgcttcagc acctgtttga ctgcgcgcga aacgcgcggc cgtcgccgcg
     6181 cttgcgccgg ccctggcccg ctttggcgta tttggcgctt cgccgggtac gcgacttggc
     6241 cttccccttc cgtggcggcg gaagatcgac gccggcacgg cgcgcttcgg agaggccgat
     6301 cgcgatggct tgctgcggcg atcgtgcgcc gtgctcgcca cgacgaatct tgtctatttc
     6361 ttcgtgcacg aactcgccgg cctgc
//
LOCUS       DATLJT010001744         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1810006,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001744 DATLJT010000000
VERSION     DATLJT010001744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810006"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1513)
                     /gene="dinB"
                     /locus_tag="VM910_39595"
     CDS             complement(266..1513)
                     /gene="dinB"
                     /locus_tag="VM910_39595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVI68634.1"
                     /translation="METTATILHADLDAFYASVEQLLDPSLRGKPIAVGGGVVLAASY
                     EAKSFGISSGMSGWRARELCPQLIFVGGHFQDYQRLGDAAINVLGDFTPLVERISIDE
                     AFADVAGCTHLFGSPPDIARAVRRRVRAELGLAISIGVARTKHLAKIASQVAKPDGLV
                     VVDPDTELDFLHDLPVGLMWGVGPVTRARLSEIGVHTIGQLAKMPGWSLERIVGRAAG
                     EKLTALAWNRDPREIRTRHRAQSAGAQSALGRKPAEVQIFRPTLRHLADRVASRLRAK
                     SRPGRTVTVRVRFADMHSVTRSVTLDAPISATMSLAEIAEELVRGALADHPEEENISL
                     LAISVSHLEKHWKLQLDLPLGLADEKRRPGAKRGIARWTADRAVDMIRDRFGWNAVGY
                     GSVVLGLSRSVPDEFRELAEKDL"
     gene            1494..2927
                     /locus_tag="VM910_39600"
     CDS             1494..2927
                     /locus_tag="VM910_39600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVI68635.1"
                     /translation="MVAVVSTPTLPLRNLGNGSIPQSLVSLCLYKNKMRTLSQMGLDI
                     SVNKCVIYRAAVLGARPRRHYFCLHTESTAFNAARPSLTWTSYERRGQSYGVLTMRRI
                     YLDYNASTPIDPAVAAAMLLFLEDHYGNPSSRHWASVEAKAAIETARGQVAALLGCHD
                     DEIVFTGGGSEANNLALKGAFFAPRDKGDHIITTRIEHPSIIEPCRFLERLGAQVTYL
                     PVDRTGRIDPDDLRRAITPRTMLISIMHANNEVGTIEPIEDCARIAHEHRILFHTDAA
                     QSVGKVSTDVNELGVDLLSVAGHKVYAPKGVGALFVRRGVRLESLIHGAAHEAGRRAG
                     TESAFLAIGLGKACELACDLAPMDLVRALRDHFWQELEQRFGNRIALNGHPTRRLPNT
                     LNVSFVGQIGTEILARLDGVAATTGSACHSGRIELSPVLEAMHIAPEVGMGAVRFSLG
                     RTTTPNEIKVVVERLTDLFAKEGQGAK"
     gene            3019..>3200
                     /locus_tag="VM910_39605"
     CDS             3019..>3200
                     /locus_tag="VM910_39605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68636.1"
                     /translation="MTTDHPTAAGTATTVMSVDLSYLVRRRIFLYLGILIVLLAFGAP
                     GGGLIDIPITFLLKNK"
BASE COUNT          681 a          968 c          897 g          654 t
ORIGIN      
        1 tgggacggag gtcgaaaaaa tctgtgatgg cactcgaaaa atgcgcgctg ttttgaaatt
       61 cactttctac tctgtgagtg accaaagctc taccctatag agccagcaag tcgtgcgtcg
      121 gatgtctccc agggttagaa ctccttttgc aacgtcatcg acccggcacc gggcgtcaga
      181 tccgtagacc cattattgac cctcgatcag ttcggcgtcg tgttcgaaaa gccatgcgat
      241 cgcggctcat agcgagccgc catgattaga ggtccttttc ggcgagttcg cgaaactcat
      301 cgggaacaga gcgggatagt cccaacacga ctgagccgta cccgaccgca ttccatccga
      361 agcgatcgcg gatcatgtcg acagcgcggt ctgccgtcca acgcgctatt cctctcttgg
      421 cgccaggccg gcgtttttca tctgcaagcc caagtggaag gtccagctgc agtttccagt
      481 gcttctcaag atgcgacacg gagattgcca gcagcgagat gttctcctct tcaggatgat
      541 cggcaagtgc gccgcgaacg agctcctcgg cgatttcggc aaggctcatg gtcgcagaaa
      601 tcggcgcatc aagggttacc gagcgcgtga ccgagtgcat gtcggcgaac cgaacccgaa
      661 cggtcacagt gcgaccgggc cgggatttcg cccgaagccg gcttgccact cggtcggcaa
      721 gatggcgtag cgtgggtcgg aatatttgca cttcagcagg cttcctgccg agcgccgact
      781 gggctccagc cgattgtgct cggtgacgcg tcctgatctc ccgtggatca cgattccagg
      841 cgagcgctgt gagcttctct ccggccgcac gaccgactat tcgctcaagc gaccaccctg
      901 gcatcttcgc cagctgcccg atggtatgga caccaatctc ggacagccgt gcccgggtaa
      961 ctggacccac tccccacatc aatccgacag gcaggtcgtg aaggaaatcc agctcggtgt
     1021 cgggatcgac aaccaccagc ccgtcaggct tggccacttg cgaggcaatt ttcgcgaggt
     1081 gcttggtgcg cgccacgccg atcgagattg caaggccaag ctctgcgcgc acgcgacgac
     1141 gcaccgctct cgcgatatca ggcggcgaac cgaaaaggtg ggtgcaccct gcaacgtcag
     1201 caaaggcctc gtcgatagaa atgcgttcga cgagaggggt gaaatcaccg agcacattga
     1261 tggcagcatc gcccagccgc tggtaatcct ggaaatgccc gccgacaaaa atcaattgcg
     1321 gacacagctc acgggcccgc catcccgaca taccgctact gatcccaaaa gatttggctt
     1381 cgtacgaggc ggcgagcacc accccgccgc caactgcgat gggtttgccg cgcagagacg
     1441 ggtcgagcag ttgctcgacc gatgcataga acgcatcgag gtctgcatgg aggatggtgg
     1501 ctgttgtctc caccccgacc ctccccctgc gaaatttggg aaacggatca attccgcaat
     1561 cgttagtgag cttgtgcctt tataagaaca aaatgagaac actgagtcaa atgggtttgg
     1621 acatcagcgt gaataaatgc gtcatctatc gcgctgccgt gctcggcgct cgcccgcgtc
     1681 gtcactattt ttgccttcac actgagtcta ccgccttcaa tgccgccaga ccgtcgctga
     1741 cttggaccag ctacgagagg cgcggacaaa gttacggtgt tctgaccatg cggcgtatct
     1801 acctcgacta caacgcgagc acaccgatcg atcctgcagt cgcggcggca atgctgctgt
     1861 ttcttgaaga ccattacggt aacccgtcaa gccggcattg ggcctcggtc gaggcaaagg
     1921 cagccatcga aaccgctcgc ggccaagtcg cagcactgct cggctgtcac gacgatgaga
     1981 tcgtttttac cggcggcggc agcgaggcca acaatctcgc cctcaagggc gcgttcttcg
     2041 cgccgcgcga caagggtgat cacattatca ccacccggat cgagcacccg tcgatcattg
     2101 aaccgtgccg ctttctcgaa cgcctaggtg cacaggtgac ttatctgcca gtcgaccgca
     2161 ctggccgcat tgatccagac gacttacgtc gagccatcac gccgcgcacc atgctcatca
     2221 gcatcatgca tgccaataac gaggtcggaa ccatcgagcc gatcgaggat tgcgcccgga
     2281 ttgcgcatga gcatcgcatt ttatttcata cggatgcggc ccaatccgtc ggcaaggtct
     2341 cgaccgatgt gaacgaactc ggtgtcgacc ttctttccgt tgccggccac aaggtttacg
     2401 cccccaaggg cgtgggcgcc ctatttgtgc gacgtggcgt acgcctggaa tcattgatcc
     2461 acggcgccgc gcacgaggcc ggccgacggg cgggaacaga gagcgctttt cttgcaatcg
     2521 gtcttggcaa ggcctgcgag cttgcatgtg atcttgcgcc gatggatctg gtgcgcgcat
     2581 tgcgcgacca tttttggcaa gagctagagc agcgatttgg aaatcgcatt gccctcaacg
     2641 ggcatcctac gcgtcggctg ccgaacaccc tcaacgtttc cttcgtcgga cagatcggca
     2701 ctgagatatt ggcacggctt gatggcgttg ccgccacaac aggatcggca tgtcattcgg
     2761 gccgcatcga gctgtcgccg gttctggaag ccatgcacat cgcgcccgaa gtcggcatgg
     2821 gtgccgtccg cttcagcctt ggacgcacga ccacaccgaa cgaaatcaaa gtggttgtcg
     2881 aacgtctgac tgatctcttc gccaaagagg gacaaggggc aaagtagcca gagcgagaag
     2941 actaaatcga ttttgatgat ggtccgcttt ttgctgtgcc ttgttcgcac gcgaccaccc
     3001 aattgcccgg caaccccaat gacgactgat catccaacgg ccgcaggaac agccactacc
     3061 gttatgtcgg tcgatttatc gtatttggta cgccggagaa tattcctcta tttaggcatc
     3121 ctcatagtac tgttggcctt cggcgcgccc ggcggtggcc tcatcgatat tccgataacg
     3181 tttctgctca agaacaaatt
//
LOCUS       DATLJT010001745         1621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1810160,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001745 DATLJT010000000
VERSION     DATLJT010001745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810160"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..>1621)
                     /locus_tag="VM910_39610"
     CDS             complement(430..>1621)
                     /locus_tag="VM910_39610"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVI68637.1"
                     /translation="EPPAVKRRRKGRPGVARKLAENPDATRRFFAEHCACGAVLSEAG
                     QELAKEYDHIDIPPIRPITTRVELFRATCPCCKARVTASAPADMPEGTPFGPGIASMI
                     AYLHGCQMVGFKRLTEVCQGLFGLTISQGAIANVLARVGEAFAAPAERIAAEVRASEV
                     IASDETSARVKGKTWWQWTFGCATAVYHVIAPTRGKCVPTDFLGGVKPQMWLSDRLAA
                     QLGHAEEHQFCLAHLIRDAQYAIDHGDTIFAPDFKALLKDACEVGRRRPALADVTIAA
                     HRRRLERQLERLLARKPTDAEGRKLRDAVYVDCSDKLFVFLKRRDAEPTNNESERALR
                     PSVIFRKVTNGFRSEWGAKTYAALCSIVETGRRNGRSALIAIRDALALPVAQAAGARA
                     THTG"
BASE COUNT          291 a          525 c          521 g          284 t
ORIGIN      
        1 gaacgcgaga ttgacgagta tctcgcaaac tgcgtcgcgc agcgcgacgc caccacggcc
       61 gcctaagaaa accccggcgc tgttagtgca gcggccgggc cagtgggttg gatatagata
      121 gcaccactgt tagccccttc gccgccgcgc tgcaacgccc atcatagggt gcacagtgat
      181 gcgcgcagat ttgcccaatc gcaagcaagc caattttcaa gaattgcccg tcgagttggc
      241 aagctcaccc gcgccccaga atttgaaatt tgagcgggcg gactggacct ccttccgcac
      301 tgtggagggg ctgatgcaga aggccggcgt cgcgaagaac aagctgcgcc gtctggttct
      361 gaaggaattg accgacaatg cgctcgacga aaatgccaag gtccgcatta gcgagcttag
      421 cgtaactact caccccgtgt gcgttgctcg cgcgccggct gcttgggcga cgggtagtgc
      481 tagggcatcg cggatggcga tgagagcgct gcgaccgttg cggcggccgg tttcgacaat
      541 cgagcagaga gcggcgtagg tcttcgcgcc ccactcggag cggaacccat tcgtcacttt
      601 gcgaaaaatc accgacggcc tcagtgcccg ctcgctttcg ttgttggtcg gctcggcgtc
      661 gcggcgcttc aggaacacga acagcttgtc gctgcagtcg acatacactg cgtctcgcag
      721 cttgcggccc tcggcatcgg tcggcttgcg agcgagcaac cgctccagct gcctttcaag
      781 acggcggcga tgcgcagcaa tggtgacgtc agcgagagcg ggccggcggc ggccgacctc
      841 acaagcgtcc ttgagcaggg ccttgaagtc cggcgcgaag atcgtatcgc cgtggtcgat
      901 ggcatactgc gcatcacgga tcagatgcgc gaggcagaac tggtgctcct cggcgtgccc
      961 caactgcgcg gccagccggt ccgacagcca catctggggc ttcacgccac cgagaaagtc
     1021 tgttggcacg cactttccgc gcgtcggcgc gatcacatga tagacagcgg tggcgcagcc
     1081 gaacgtccac tgccaccagg tcttgccctt cacacgcgca ctcgtctcgt cgctggcaat
     1141 cacctcgctt gcgcgcacct ctgctgcgat ccgctctgca ggcgctgcaa atgcctcgcc
     1201 gacgcgggcg agcacgttgg cgatggcgcc ctgagagatt gtgaggccga agagcccctg
     1261 gcagacctcg gtgagccgct tgaagccgac catctggcag ccgtgcaggt aggcgatcat
     1321 cgacgcgatg cccggaccga atggcgtgcc ttcgggcata tccgcggggg cgctcgccgt
     1381 cacccgcgcc ttacagcagg ggcaggtggc gcggaacaac tcgacgcgcg tcgtgatcgg
     1441 cctgatcggc ggaatgtcaa tatgatcgta ctccttcgcg agttcctgtc cggcttcgct
     1501 gagcacggca ccgcaggcac agtgctcagc gaagaagcgt cgcgtcgcgt cgggattctc
     1561 ggccagcttg cgcgcgacgc cgggccggcc tttgcgccgc cgcttgaccg ccggcggctc
     1621 c
//
LOCUS       DATLJT010001746         6063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4105295,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001746 DATLJT010000000
VERSION     DATLJT010001746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4105295"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(650..1576)
                     /locus_tag="VM910_39615"
     CDS             complement(650..1576)
                     /locus_tag="VM910_39615"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVI68638.1"
                     /translation="MEPAWRLAEEAPELRWLVTDLWSHDAVGIVGGEPKCCKSFLALD
                     LAVAVAAGVPCLRRFVVPDPGRVLLYAAEDSPAIVRRRLESIAAAAGVELSDLDIQVI
                     TAPTLRLDLEADQRSLAQTIAHFRPRLLILDPFVRLHRIDENASGEVAPLLAYLRALQ
                     RRYALAVVVVHHAKKGAAQARGGQALRGSSEFHAWGDSNLYLRRQGDDLTLTIEHRAA
                     PSQKPMKIELCQSGEALALQVVEQTAPSKPVPHSLDERITTALAAAASPLPFAELRSQ
                     CRVRNATLYEHLAALITSGRIVKDGEGYRFVS"
     gene            complement(1588..2427)
                     /locus_tag="VM910_39620"
     CDS             complement(1588..2427)
                     /locus_tag="VM910_39620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVI68639.1"
                     /translation="MTKKLLALYGLKWNPFAPDVPVEAIYLTPRTQSFCWRVEQLAGE
                     GGFALITGAPGTGKSVALRILADRLGGQRDVKIGILSRPQAGIVDFFRELGDLFSVAL
                     RPNNRWGGSKLLRERWQTYIDASLSRPVLIVDEAQEMVPSVLAELRLLSSVRLDSHIL
                     LTVVLAGDGRLVDRFRSDELLPLGSRIRVRLALERASSDELQECLRHALNKAGAPKLM
                     TPELIATLADHAQGNLRTLMNMAAELLAIAAEREARHIDEKLFLETYAQPPPEKIAGG
                     RRR"
     gene            complement(2424..3887)
                     /locus_tag="VM910_39625"
     CDS             complement(2424..3887)
                     /locus_tag="VM910_39625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HVI68640.1"
                     /translation="MDEVPDRRIHGRWARFRFTVIGHLLASPPGKGDLAGAIASLAAR
                     TWRHPISGEPVRFGFSTIERWYYRALKEKSDPVGVLRRKLRADAGQQPAMRAPVRQAI
                     FEQYAAHKSWSAQLHHDNLVALAECRRPELRPVPSYDTLRRFLKANGLAKQRRITSRR
                     TAGAERAEARFLDREVRSYEASHVGSLVHFDCHFGSKKVLTSRGELLTPILFAVLDDR
                     SRVACHLQWYLSETAENVAHGLSQAFQKRGLPRAGLSDNGAAMTAAEIREGLSRLGVL
                     HETTLPYTPAANGKVETLWASVEGRLIAMLEHVPDLTLAFLNEATLAWAEQDYNRKIH
                     SEIGVAPITRFLAGPSVMRPSPDSGALRLAFTRTETRTQRRSDGTIVIGGRRFEVPDR
                     YRHLSRLEIRYASWDLSSVHLVDERTGTVLCRLFPQDKTQNASGLRRTRDPLSKEPPA
                     TPPAAVGIPPLLDKLLKQQADTGLPPAYLPKDERDDR"
     gene            complement(3979..4548)
                     /locus_tag="VM910_39630"
     CDS             complement(3979..4548)
                     /locus_tag="VM910_39630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68641.1"
                     /translation="MYSTLLGDATFHDLLLACDEDLADEARSQRCLICCSALHSADYP
                     RKPRGRLCRLGPEHDWRFSLCCARHGCRKRRTPPSLRFLGPKVYLAAAIIVIAILREG
                     ATTTRLRRLQELIRVDRHTVERWRRWWRFLFTATPFWRIARAAFMPPVDEGALPAALL
                     ERFSGSATERMRALLRFLSPLTGGQVQAF"
     gene            4584..5093
                     /locus_tag="VM910_39635"
     CDS             4584..5093
                     /locus_tag="VM910_39635"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HVI68642.1"
                     /translation="MAKAGRNDPCPCGSGKKYKKCCQPKDEAAGHEELFKEQAKREER
                     NTARRAELQRMKEQAIAELLGNGLDNTEEEDELTKASNAAVDLVHAGKLEEAEAAARD
                     LIERYPEVHDGRDRLGMMYEAKGEAKTAADCYRKALEIIRERPGQYDPEFAAAFVELI
                     EKLDPPAPT"
BASE COUNT         1160 a         1876 c         1970 g         1057 t
ORIGIN      
        1 ggcgtagttc ggcgctcggc ataaagcccg ctataccgca cgcggcataa cgggctttcg
       61 ctgcacgctc cgtcctgaca tgccatcccc tgagaggcct gccttcatct ccagtacagt
      121 tacgcagcgc cgtttggacc ggcgttactc gtgacacaag acccgcagcc gagatcgcac
      181 gtctcggtac tttgcgatgc agcaatcact tcagtactag gtgtgacggt tgaattgcca
      241 caaattggag ggggtgagca ggctgacggt gtttgatgtt ggtgccagcg aggtaattga
      301 cgggcggcag ttgcgatgtg tctcgcccag tgccgcgtga gcatgccgtc gtgctggcgt
      361 gcgtcaagga cgagcctggc ggtggcgcta atgcgccatc cttgacgcgc cgctgcgcgc
      421 gccggcgctc tcattcgagc ggccgggacg agggaatggc tccgccgggg ccgaaccaga
      481 gaattgggac gagaagaaca agaagaatgc gagatggtcg tctctggacg gggcggcttc
      541 ccagttcccg ttcccgcaca ttgtagagcg ctcgggaagc ggaagtggga agttccccca
      601 cgatgagacg cttggtctct cgcacctgtc gcggcagcag ccgaggccgt caagagacga
      661 accgatagcc ctcgccgtct ttcactatgc ggccggacgt gatgagcgcg gcaaggtgct
      721 cgtaaagggt tgcgttgcgc acgcggcact gcgacctgag ctcggcgaag ggcagcgggg
      781 acgcagcagc tgcgagcgcc gtcgtgatgc gctcatcgag cgagtgtggc accggctttg
      841 agggagcagt ctgctccacg acctggaggg ccagcgcctc gcccgattgg caaagctcaa
      901 tcttcattgg cttctgcgag ggagcggcgc gatgttcaat tgtcaaggtg agatcgtcgc
      961 cctggcggcg cagatagaga ttgctgtcac cccaggcgtg gaactcggaa gagccgcgca
     1021 gcgcctggcc gccgcgggcc tgagcggcac ccttcttggc atggtggacg acgacaacgg
     1081 caagcgcata ccggcgctgc agcgctctca agtaggcgag cagcggtgcc acctcgccgc
     1141 tggcgttctc gtcgatccgg tgcagccgca cgaagggatc gaggatgagg agccgcggcc
     1201 ggaagtgggc aatggtctgc gccaggctgc gctgatcggc ctcgagatcg agccgcaggg
     1261 tgggcgcggt gatgacctgg atgtcgagat cggagagctc gaccccagcc gccgcggcaa
     1321 tgctctcgag ccgccgacgc acaatggctg gggagtcttc ggcggcatag aggagaacgc
     1381 ggccagggtc gggcaccacg aagcggcgca agcagggaac gccggcggcc acggcgacgg
     1441 cgagatcgag cgccaggaac gacttgcagc acttgggctc accgccgacg atgccgacgg
     1501 catcgtggga ccacagatcg gtgacgagcc agcgcagctc cggggcctct tcggccaggc
     1561 gccaggccgg ttccacgggc agaagcctca tcggcgccgc cctcctgcga tcttctcagg
     1621 cggcggttgg gcgtaagtct cgaggaagag cttctcgtcg atgtggcggg cctcgcgctc
     1681 ggccgcgatg gcgagcagct cggcggccat gttcatgagc gtgcgcaaat tgccttgagc
     1741 gtggtcggcg agcgtagcaa tgagttcggg ggtcatgagc ttgggtgcac ccgccttgtt
     1801 aagggcatgc ctgaggcact cctgcagctc atcggagctg gcacgctcca gggcgagccg
     1861 cacacggatg cggctgccca aggggaggag ctcatcggaa cggaatcggt caacgagccg
     1921 gccgtcaccg gcgagcacca cggtcaagag gatgtggctg tcgagacgga cggaggagag
     1981 caatcgcagt tcggcaagga cgctgggtac catctcctgc gcctcgtcga cgatcagcac
     2041 aggtcgagag agggaagcat cgatgtaggt ctgccagcgc tcgcgcaaga gcttcgagcc
     2101 gccccagcgg ttgttggggc gcagtgcgac ggagaacaga tctccaagtt cgcggaaaaa
     2161 atccacaatg cccgcctgcg ggcggctcaa tatgccgatc ttcacgtcgc gctggccccc
     2221 gagccgatcg gcaaggatgc gcagggcgac ggatttgccg gtgcccggtg cgccggtgat
     2281 gagggcaaag ccgccctccc cggcgagctg ctcgacgcgc cagcagaagg actgggtgcg
     2341 cggggtcaga tagatcgcct cgacggggac gtcgggggcg aaggggttcc acttgaggcc
     2401 gtagagcgcc agcagctttt tggtcatcgg tcgtctcgtt catccttggg gagataggcc
     2461 ggtggcagtc cggtgtcagc ctgctgcttc aagagcttgt caagcagcgg cggaatgccc
     2521 actgcggccg gtggagtggc cgggggttcc ttggaaagcg gatcgcgcgt cctgcgcaga
     2581 ccgctggcgt tttgcgtctt gtcttggggg aagagccggc acagaacggt gccggtgcgc
     2641 tcgtctacga ggtgaacgga gctgagatcc cagctggcgt agcggatctc gagccgtgag
     2701 agatggcggt agcggtccgg cacctcgaag cgacggcccc cgatgacaat ggtgccgtcg
     2761 ctcctcctct gcgtgcgggt ctcggtcctg gtgaaggcga gccgcagcgc gccgctgtca
     2821 ggggagggtc gcattacgct cggccccgcc aggaagcgcg tgatgggagc aacgccgatc
     2881 tcggagtgga tcttgcggtt gtagtcctgc tcggcccagg cgagggtcgc ctcattgagg
     2941 aaggcgagtg tgagatcggg gacgtgctcg agcatggcaa tgagccgacc ctccacggaa
     3001 gcccagagcg tctcgacctt gccattggct gccggcgtat aaggcagggt cgtctcgtgc
     3061 aggacgccga gacgagaaag tccctctctg atctcggccg ccgtcatggc ggcaccgttg
     3121 tcgctcaggc ctgcacgcgg cagtccccgc ttctggaagg cttgggagag gccatgcgcg
     3181 acgttctctg cggtctcgga gaggtaccat tgcagatggc aggcaactcg ggagcgatca
     3241 tcgagaacgg cgaacaagat tggggtcagc aactcgccgc gggaagtgag taccttcttg
     3301 gaaccaaaat ggcagtcaaa atgaacgagg cttcccacgt gactggcctc gtagctcctc
     3361 acctcacgat cgaggaaccg ggcctcggcg cgttcggcgc cggcggttcg gcgtgatgtg
     3421 atccgtcgct gctttgcaag cccgttggcc ttgaggaagc gacgcaacgt gtcatagctg
     3481 gggacgggtc tgagttcagg acggcggcac tcggccagcg caacgagatt gtcgtggtgc
     3541 agctgcgcac tccaactctt gtgcgccgca tattgctcaa agatcgcctg gcgtacgggt
     3601 gcgcgcatcg cgggctgctg gccagcatcg gcgcgcagct tacggcgcag gacgcctacg
     3661 ggatcgctct tctccttcaa ggcgcggtaa taccaacgct cgatggttga gaagccgaag
     3721 cggacgggtt cgcccgagat ggggtggcgc caggtgcgcg cggcgagcga cgcgatcgcg
     3781 ccggcgaggt cgcctttccc cggcggtgac gccaataggt gcccgatcac ggtgaagcga
     3841 aaccgcgccc accgcccgtg aatgcgtcgg tcgggtacct catccatcgt ggctcgtgct
     3901 cccgttcaac gagagcgcga agttaagaga gtgccccgat tagttcgacg cgcgtcttct
     3961 gcggatttgc gagagccgtt agaaagcctg cacttgccct ccggtcagcg gcgacagaaa
     4021 gcgcaacagc gcccgcatcc gctcggtcgc gctgccagaa aagcgctcga ggagcgcagc
     4081 cggcagcgcg ccttcatcga ccggcggcat gaaagcggcg cgcgcgatcc tccagaaggg
     4141 cgtcgccgtg aagagaaagc gccaccacct cctccagcgc tcgaccgtgt ggcggtcgac
     4201 gcggataagc tcttggagcc ggcgcaaccg ggttgtggtc gcgccttccc gcaggattgc
     4261 aatcacgatg atggctgccg cgagatagac cttggggcca agaaagcgca gagagggtgg
     4321 cgtcctgcgc ttgcgacagc catgacgggc acagcacaag ctgaagcgcc aatcgtgctc
     4381 gggccccaaa cggcagagcc ggccgcgcgg tttgcgcgga tagtcggccg aatgcagcgc
     4441 gctgcaacag atcaggcagc gctggctgcg cgcctcatcg gcgaggtcct catcacaggc
     4501 cagcagcaga tcatgaaagg tggcgtcgcc cagcagggtc gaatacaagg atacctccct
     4561 caaactccaa ggcattgacc agaatggcca aagcgggacg caatgatccc tgcccctgtg
     4621 gcagcggtaa gaagtacaag aagtgctgcc agccaaagga tgaggcggcc gggcacgagg
     4681 agctcttcaa ggagcaggcc aagcgcgagg agcggaatac ggcgcgccgt gccgaactcc
     4741 aacgcatgaa ggaacaagcc attgccgagc tcctcggcaa tggcctcgac aataccgagg
     4801 aagaagacga gctgaccaag gcctccaatg ccgccgtcga tctcgtgcac gccggcaagc
     4861 tcgaggaagc cgaggccgcc gctcgcgatc tgattgagcg gtatcccgaa gtccacgacg
     4921 gccgggatcg cctcggaatg atgtacgagg ccaagggcga ggcgaagacc gccgcggatt
     4981 gctatcgcaa ggcactcgag atcattcgcg agcgcccggg acagtacgac ccagagttcg
     5041 cggccgcatt cgtcgagctc atcgaaaagc tcgatcctcc agccccgacc taacaccaca
     5101 attggtcgtt ctgcgctcga tcatagaggg cgccgcgata tcggccatca caaaccttcc
     5161 caaaatcaat cggcgatgac cctcacgtta cgtgccgggt ctcatcaccc tgccccatct
     5221 cttcgtccgc gttcccagaa ctgcttatcg aggcacccat gtcccattag gaccgaactc
     5281 gcgtacgcca taacgaccag aagcgcgatt acgctcccga tcagcaaaat ccgattccaa
     5341 tccatctctg ctcaaccccg cgctcgcacg ctcgcgccca ttaaaattcg tgagccacac
     5401 cgcttgcaat ttccctgcat ttcccttcca aaatccgtgc actcacccca gtgcgttcat
     5461 tcctctgcca ctctaaaatt ccgatgccca gcaaagctca agctgttgtt gtcgagcaac
     5521 gcgtcttgat tagaaatcaa tttctttaac ttaagactgc ttcattcatt gatccggacc
     5581 ccagattcca ccaccgtcca ttctgctggg cgttcgcgtt ctgatcgcct ccactttcga
     5641 gtcggaacta tgagcagcca gttcgaaact ttcctgcatg tcgccgaggt agcatgtgct
     5701 gccggcgttc tcgcttggat cttcgcgctt cttacttggc ggcgcgcgct ttcacatcgt
     5761 gcactggctt tccttcttac aagcgtcgtg ctcctcatac ttctcggagc cccaccggcc
     5821 gcgctcgcga cgggcttcgt ctgcgttgtc gttatcgtcc tactcgaatc tacgcaatgg
     5881 cgcgcggcag aaccgccgaa taaataaccg aaacagccgc gacctccacc gcaagacagc
     5941 tccgccgctc acccacccta gaacgcggcc aaccgtacgc tcatcgtctc gcgctcgcac
     6001 gctcgcgctc ccagcagaat tcatgacgcg ttccgcatcc aatttaattt acgtgacgtc
     6061 att
//
LOCUS       DATLJT010001747         6305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1349922,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001747 DATLJT010000000
VERSION     DATLJT010001747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349922"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VM910_39640"
     CDS             complement(<1..763)
                     /locus_tag="VM910_39640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVI68643.1"
                     /translation="MLQANEGFTSHFTDQQFRVRYAVRHGENGDVEYLRRGTDGKWDR
                     FARISADDAVATRAIEFSADGRELYWLDSRGRDTAAVVAHDLESDTTRILAEDPRADF
                     TYLLLDPITERPVAAARTFERIRWEVLDPDYEEDFNYLTRQSSGDLMITSMSQDRRHW
                     IVAYLHDNAPLEYFHYDRNARQARRLFSSMPALEGAPLVMMEPVIVRTRDGLELVCYL
                     SRPRDARPAKRLPMVLLVHGGPWTRDVWALYPDHQW"
     gene            986..1237
                     /locus_tag="VM910_39645"
     CDS             986..1237
                     /locus_tag="VM910_39645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68644.1"
                     /translation="MPIVVHPYDRGAEITIGHRGHGTRIVYLVDGGNPKIEHAIYRRE
                     EGNPRAIRTYAHDAPFGISEDYAARKQPGAFKHGGVMAL"
     gene            complement(1282..1830)
                     /locus_tag="VM910_39650"
     CDS             complement(1282..1830)
                     /locus_tag="VM910_39650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68645.1"
                     /translation="MFEYCHQPDCILRAQLSRLSSDVLLSDGTFGRAGDRVIDLHFWN
                     EQIPVKPIAGYSLAWGCRFNQSLGKSLRGLAQFLMSKPQLSDINIIRAITNLDSLHRI
                     AARHGFTAIRDPVNPSLWEHVHRFGQNILYWLLTLACHSGRARPGKFSRTRQVIYLSR
                     RGLDCKYIAAARGHNHDVHGLA"
     gene            complement(2103..3353)
                     /locus_tag="VM910_39655"
     CDS             complement(2103..3353)
                     /locus_tag="VM910_39655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVI68646.1"
                     /translation="MMIESHASSRRELLLASGALFAWTYMPKLARAEGRDPRFLTIVL
                     RGALDGLAAVAPVGDPHWVSLRGDNALTLDSKTSALKLDDFFALNPAMPNLHRMFKAN
                     EAIVVHAAATSYRERSHFDGQDVLESGVEKPGPTRSGWLNRLLGGLAPGDRVSSNGAR
                     AFAVGPVAPLVVRGPAPALSWWPRRVLPASDDTIMRLLDLYRHTDVNFARVLEGRTKL
                     TAIAPAGSMDQKPIGQIASPDVAQLRAYFAEAVGTAAKFLAQPDGPRVGALALNGWDT
                     HYNEGIATGWLAQLLGALDAALAAVQENMGPAWRETVVVLVTEFGRTARINGTNGTDH
                     GTATVMLLAGGALKGGRVIADWPGLKAENLLENRDLKPTTDVRAVLKGLLKDHLRADD
                     GALAADVFPGSDDVKPIAGLVTSA"
     gene            complement(3354..4811)
                     /locus_tag="VM910_39660"
     CDS             complement(3354..4811)
                     /locus_tag="VM910_39660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HVI68647.1"
                     /translation="MTTNPRLEAALALHRFGFGPVAGSIAAIADDPRGALLADLERPG
                     AGRVAAANLPNSGQAARAFFEFRAERQAKEKLAERAKKETEAKGIVVGAADDSAMKSN
                     GDASAAKPEPAQTRQGQQPPPLPTQLVQNEAKIRIEAAVAAEIGLVERLVWFWSNHFC
                     VSADKILSMAGAYEREAIRPHVLGRFVDMLTAVESHPAMLVYLDNLESMGPNSVAGIN
                     RDKSLNENLAREILELHTLGVRTGYSQDDVTHFANVLTGWTWVGLGEPNHGGEFVFVK
                     RLHEPGEQIVIGKSYPDAGVDQGRAVLADLARQPATAGHIAHKFARHFVADAPPPSLV
                     AKLTKSFTDSDGNLKELAKTLIAAEESWTPERTKLKRPSEWIVSALRLTGAQWAAGRI
                     MSGQALLGEPLWRPPAPNGFAEDESTWIDGLSQRVDIASNFAERVADRLDPVALIETG
                     LGRLASAETRDTVARAEGRAQALALLLMAPEFLRR"
     gene            5091..>6305
                     /locus_tag="VM910_39665"
     CDS             5091..>6305
                     /locus_tag="VM910_39665"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HVI68648.1"
                     /translation="MSKLSRVTLRLLGAFAIEANVGGRPIPISVRSRKARALLAYLAM
                     KPDYRARREELATLFWGDNPDALARHSLRQCLISLRQDLCLASEILVVDREAIGLRTQ
                     FVCVDARRFMSLARSAGSDGLAQAAALWQGTFLPDLALDIEEFDTWHRQEADRLSAVA
                     ADVFEALCRNADTNGGGERAIAAAERLIALEPTREDRQRTALMLFARYKGREAALSRA
                     RQFTDLLRSELDVSPEAATRALIDAIKRGDFEPADAPHRAQPAAQSVVKPVSLPDRVA
                     RSLPCEKASSVPAPAVLTAAPTPRNTASATPSFWRRQPFAAACVLVAFLAMGTIVANT
                     SKLWSPQTDLQRGQALVVLPFAVDNSGQPDDPAFARVLTHNLIGYLSRFGKLRVISEQ
                     TSDFYRERQTDVA"
BASE COUNT         1156 a         1980 c         1974 g         1195 t
ORIGIN      
        1 gccattggtg atcggggtac agcgcccata cgtcccgtgt ccacggcccg ccatgtacga
       61 gcagcaccat cggcagcctc ttcgccggcc gcgcgtcgcg cggacgggac agatagcaga
      121 cgagttcgag tccgtcgcgg gtgcgaacga tgacgggctc catcatcaca agcggcgctc
      181 cctccaatgc gggcatcgac gaaaagaggc gccgagcctg cctcgcgttg cgatcgtaat
      241 ggaagtattc caaaggtgcg ttgtcatgca gataggctac gatccagtgc cgccggtcct
      301 gcgacatgct ggtgatcatg aggtcacccg acgattggcg cgtgaggtag ttgaaatctt
      361 cctcgtagtc cgggtccagc acctcccacc ggatacgctc gaatgtacgt gctgctgcga
      421 ccgggcgctc ggttatgggg tcgagcaaga gataggtgaa atcggctcgc gggtcttccg
      481 ccaggatccg cgtcgtatca ctttcgaggt catgcgcgac aactgcggcg gtatcgcgac
      541 cccggctatc cagccaatat aattcgcggc cgtcggcgct gaactctatg gcgcgggtcg
      601 ccaccgcatc gtccgcgctg attctggcga agcggtccca tttaccgtct gtgccgcgcc
      661 gcaggtactc gacatcgccg ttctcgccat gacgaacagc gtagcgcacg cggaactgct
      721 gatcggtgaa gtgcgaggtg aaaccctcgt tggcttggag caaagtgctt tccccggtcg
      781 caacatctac acgatagacg tcgaagtaac gcttatcgcg ctcgttgtgc gcgataagca
      841 gttcgctggg aaagtggagc gaactctgct gaacgtagca tctcaccccg ggtcccggag
      901 tcagcggcct gattttggca gttcggagat cgactcgcca ggcgcgccag ttctcgtcgc
      961 cagcctgctc gcggaagaat acgatatgcc gattgtcgtg catccatacg atcgaggagc
     1021 cgagattacg atcggtcacc gcggtcacgg gacgcgcatt gtctatctgg tcgatggggg
     1081 caacccaaag attgagcacg ccatctaccg gcgcgaggaa ggcaatccgc gcgccatccg
     1141 gacttatgcg cacgacgcac cgttcgggat ttccgaagat tacgcggcgc ggaagcagcc
     1201 cggagccttt aagcatggcg gcgtgatggc gctgtaagca tggcggcgtg acggcactgt
     1261 tcgggcatga gcttcgtggc gttaagcgag tccatgaaca tcatggttat gccccctcgc
     1321 agcggcaatg tacttgcaat cgagtcccct gcgagagaga taaatgacct gtcgagttcg
     1381 cgaaaattta cccggccgcg cacggccgga atgacacgca agcgtcaaca gccaatacag
     1441 aatattttgg ccgaaccgat gcacgtgctc ccaaagcgaa ggattgacag ggtcgcgaat
     1501 ggccgtgaat ccatggcgcg cggcgatgcg atgaagcgag tcgagatttg taatcgcgcg
     1561 gatgatgttt atgtccgaga gttgcggctt gctcatcaga aattgcgcta atccgcgaag
     1621 ggacttcccg aggctctggt tgaaacggca tccccacgcc agggaatagc ctgcgatagg
     1681 tttgaccggg atctgctcat tccagaagtg gagatcgatc actcggtcac cagcgcgacc
     1741 gaacgttccg tcggaaagca gcacatcact ggacagtcgg ctgagctgcg cccgaagaat
     1801 gcagtccggc tggtggcagt actcgaacac gccctcccgc cctcgcaacc gcgtatcgag
     1861 agcaaaaatg aactccgccc agagcggccg cgtgcggttt gctccaggtc gattctcaac
     1921 gcgcgaggaa catacgcgaa atgcccacgg ctggtcccat cggttgaaaa tcgtcagatg
     1981 gcatcgggat aaaagtgtca tagcgccccc ttgcagttcg ttacagtccg tcggcagcga
     2041 gcaacgcgac tgcgcttgag cattccgctc tctccttgga gagcggattc aactcaacgc
     2101 ggtcaggccg acgtgaccaa gcctgcaata ggcttcacat cgtcgctgcc ggggaacaca
     2161 tcggctgcga gcgcgccgtc atccgcccga aggtgatcct tcagcaatcc tttgagcacg
     2221 gcgcgcacat cggtggtggg cttgaggtcg cggttctcca acaagttctc cgccttgagc
     2281 ccaggccagt cggcgatcac gcggccgcct ttgagcgcgc cgccggcgag cagcatgacg
     2341 gtggcggtgc cgtggtcggt gccattcgta ccgttgatgc gcgccgtgcg gccgaattcg
     2401 gtgacgagca ccaccacggt ctcgcgccac gccggtccca tgttttcctg gacggcggcg
     2461 agagccgcat cgagcgcgcc cagcagttgc gccagccagc cagttgcgat gccttcgttg
     2521 taatgtgtgt cccagccgtt gagcgcgagc gcgccgacgc gcggaccgtc gggctgcgcc
     2581 aggaacttcg cggcggtgcc aacggcttcg gcgaaatagg cacgcaactg cgcgacgtcg
     2641 gggctggcaa tctggccgat cggcttctgg tccatgctgc cggcgggcgc aattgccgtg
     2701 agcttcgtgc ggccttcaag gacacgcgcg aagttgacgt cggtgtgccg atagagatcc
     2761 agcaatcgca taatcgtgtc gtcgcttgcc ggcagaaccc gccgcggcca ccatgacagc
     2821 gccggcgctg gtccacgaac gaccaatggc gccaccggtc cgaccgcgaa ggccctggcg
     2881 ccgttcgaac tgacccggtc ccccggcgcg aggcccccca gcaaacggtt gagccacccg
     2941 ctgcgggtcg ggcccggctt ttcgacgccg ctctcgagca cgtcctggcc atcgaaatgc
     3001 gagcgctcgc gataggaggt ggcagcggcg tgaacgacaa tcgcctcgtt cgctttgaac
     3061 atacgatgca gattcggcat cgctggattg agcgcgaaga aatcgtcgag cttgagtgct
     3121 gatgtcttgc tgtcgagcgt cagcgcattg tcgccgcgca gcgaaaccca atgcggatcc
     3181 ccaaccggag ccaccgccgc gaggccgtcg agcgccccgc gcagcacgat cgtcagaaag
     3241 cgcggatctc gcccttcggc ccgcgcgagc ttcggcatat acgtccaggc aaacagagcg
     3301 cccgaagcga gcagcaattc ccggcgcgac gatgcgtggc tttcgatcat gatttatctc
     3361 cgcaggaatt ctggcgccat cagcagcagg gcgagggcct gcgcgcgacc ttccgcgcga
     3421 gcgaccgtgt cgcgcgtctc cgccgacgcc agccgaccga gcccggtctc aataagcgcc
     3481 accggatcca gccggtcagc cacgcgctca gcgaaattgc tggctatgtc gacgcgttgc
     3541 gacaggccat cgatccatgt cgactcatct tcggcgaagc cgtttggagc cggcggacgc
     3601 cacagcggct cgccgagcaa cgcctgcccc gacatgatac ggccggccgc ccattgcgcc
     3661 cccgtgaggc gcagcgccga tacgatccat tcggatggac gcttgagctt cgtacgctcg
     3721 ggcgtccatg actcctcggc cgcaatcagc gtcttggcca gctcctttag attgccgtca
     3781 ctgtcggtaa agctcttcgt cagcttggcg accagcgacg gcggaggcgc gtcggcgacg
     3841 aaatgccggg cgaacttatg cgcgatgtgc ccggcagtcg ccggctgccg cgcgagatct
     3901 gcgagcacgg cacggccctg atcgaccccc gcgtcgggat agctcttgcc gatcacaatc
     3961 tgttcgcccg gttcgtgcag gcgcttgacg aaaacgaact ccccgccatg gttgggctcg
     4021 ccaagcccca cccaggtcca cccggtgagc acattggcga agtgcgtgac gtcgtcctgc
     4081 gaatacccgg tgcggacgcc gagcgtgtgc agctccagaa tttcgcgagc taggttttcg
     4141 ttcaagctct tgtcgcggtt gatgccggcg accgaattcg ggcccatgga ttccaggtta
     4201 tcgagataaa ccaacatggc cggatggctt tcgaccgcgg tcagcatgtc gacgaagcgg
     4261 ccaaggacgt gcgggcgtat cgcctcgcgt tcataggcac cggccatcga aaggattttg
     4321 tctgccgaaa cacagaagtg attcgaccaa aaccaaacaa ggcgctcgac taagccgatc
     4381 tcggcggcga cggcggcttc gatgcggatt ttggcctcgt tttgaacgag ctgcgtcggc
     4441 agtggcggcg gctgctggcc ctgccgggtc tgagccggtt ccggcttggc agccgacgca
     4501 tccccgtttg atttcatcgc actgtcgtcg gccgcgccaa caactatgcc cttcgcctcc
     4561 gtttccttct tggcgcgctc cgccagcttt tccttcgctt gccgttcggc gcgaaactcg
     4621 aaaaaggcac gcgccgcctg cccgctgttg ggcagattcg ctgctgcgac ccgcccggcg
     4681 ccaggccgtt cgaggtcggc cagcaatgcg ccgcgtggat cgtctgcgat tgccgcgatt
     4741 gaaccggcga ccggtccgaa accgaaccgg tgcaatgcga gtgctgcttc gagtctgggg
     4801 ttggtcgtca tgttgtcgtc ccgggccagc tcatcgatcc tgcggcgggc gcatctgcgc
     4861 tcgatcagcc gcaagcgcag agcagccttc gaatttcatc aacgatggca cggccgtggt
     4921 catcgcggcg tcattgcgcc gtcaaaggaa acctttcgat ggatgaggta cttaatgcgc
     4981 aggcaaggtt ctcaaggacc gttctccagg cctttgcgct tgagcttggt gcgtgcggat
     5041 caagagtgat aagttaccta gccgtggatt cttcggcggg gctccgatcg atgtccaaat
     5101 tgagccgcgt cacattgcgt ctgctcgggg cgttcgcgat cgaagcgaac gtgggcggcc
     5161 gccctattcc gatttcggtc cgatccagaa aggctcgagc gctgctcgcc tatctggcga
     5221 tgaagccgga ctaccgcgcg aggcgggaag aactcgccac actgttttgg ggtgacaatc
     5281 ctgatgcgct ggcgcgccac agcctgcggc aatgcctcat ttcgctgcgc caggatttgt
     5341 gcctggcatc tgaaatcctc gtcgtggacc gagaggcgat tgggctacgc actcaattcg
     5401 tctgcgtaga tgcgcgtcgg ttcatgtcac ttgctcgatc ggcggggtcg gacggactgg
     5461 cccaggccgc ggcgctctgg cagggaacgt ttttgccgga cctcgctttg gacatcgaag
     5521 agttcgatac ctggcatcgt caagaagctg atcggctttc ggcggtggcg gcggacgtct
     5581 tcgaagcgct ttgccggaac gccgatacga atggcggtgg cgaacgcgcc attgcggcgg
     5641 ctgaacgtct aattgcgctg gagccgacgc gcgaggatcg acagcgtacc gcgctgatgc
     5701 tgtttgcgcg ctacaaggga cgcgaagccg cgctgagtcg ggcaaggcag ttcactgatc
     5761 tgctgcgcag cgaacttgac gtttctcccg aagcagcaac gcgcgccctg atcgatgcga
     5821 tcaagcgcgg tgattttgaa cctgcggatg cgcctcatcg tgcgcagcct gcggcgcaga
     5881 gcgttgtcaa accggtcagc ctgccggaca gggtggcgcg ttcgctgccg tgcgagaagg
     5941 cgtcctcggt tcccgcaccg gccgtgctta ccgccgcgcc aacgccccgc aatacggcgt
     6001 cggccacccc gtcgttttgg cgtcgccagc cattcgccgc tgcatgcgtg ctcgtcgctt
     6061 ttctcgcgat gggcacgatc gtcgccaaca catcgaaact atggtcgccg cagaccgatc
     6121 tgcagcgcgg ccaagcgctt gtggttctgc cctttgccgt cgacaactcc gggcagccag
     6181 acgatcccgc gttcgcgcgc gtcctcaccc ataacctgat cgggtatctc agccgctttg
     6241 gcaaattgcg tgtgatctcg gaacagacat ccgacttcta tcgggagcgt cagacggacg
     6301 tggca
//
LOCUS       DATLJT010001748         5956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3418421,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001748 DATLJT010000000
VERSION     DATLJT010001748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3418421"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="flgF"
                     /locus_tag="VM910_39670"
     CDS             complement(<1..735)
                     /gene="flgF"
                     /locus_tag="VM910_39670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HVI68649.1"
                     /translation="MQNTLLVGLSRQATLERQLDVISNNVANVNTNGFKADQMLFEEY
                     LTSRAHEDNFQGADRRVSYVQDRGTYRDYTKGAMQLTGNPLDVAIDGSGFLAVQAAGG
                     ERYTRDGNLQINSQGQLVTNDGNPVLGQSGPIVFQSTDHDINISPDGTITVLEGASRI
                     DSIRGKMRVVNFADAQKLLKQGYNLFAAGDGAAQADTKSAVRQGFVEKSNVNVVSEMS
                     HMIEVMRTYTNISNLLQQQGDLHKNAI"
     gene            1120..1617
                     /gene="fliL"
                     /locus_tag="VM910_39675"
     CDS             1120..1617
                     /gene="fliL"
                     /locus_tag="VM910_39675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HVI68650.1"
                     /translation="MADNEQAEGSAETSEVTAQPKSKFKLIVMIVAALFVLGAGAATW
                     FLFFRHPADEVHAEAPPPKPPVFVEVPDMMVNLVTGPGERVQYLKVKVVLEVKEERQV
                     EEIKPTMPRVTDIFQTYLRELRPSDLTGSAGLFRLKEELTRRVNAAVAPTKVSAVLFK
                     EIMVQ"
     gene            1634..2836
                     /gene="fliM"
                     /locus_tag="VM910_39680"
     CDS             1634..2836
                     /gene="fliM"
                     /locus_tag="VM910_39680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HVI68651.1"
                     /translation="MAGNDPVDQDAIAAQWEASLGSEDPVAAAEAAESNELTESMALQ
                     WAAMVEDGSRDFGAGKNSGERVLSQEEIDNLLGFTVGDVNLDDHSGIRAIIDSAMVSY
                     ERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVISVFK
                     AEEWENFGLATVDSSLIYSIIDVLLGGRRGNSTLRIEGRPYTTIETNLVKRLIEVVLS
                     DAEQAFRPLSPVTFSIDRLETNPRFAAISRPANAAILVRLRIDMEDRGGNIELLLPYA
                     TIEPIRSVLLQMFMGEKFGRDPIWEGHFATEIAQAEIAVDAVLYESDIPLKELMKLKV
                     GDTLPLDMRADALVSVRCGSVTLTEGRMGRVGDRVAIRVTKPLRKPRTTLAMFEKADE
                     QNKLMEAQ"
     gene            2833..3315
                     /locus_tag="VM910_39685"
     CDS             2833..3315
                     /locus_tag="VM910_39685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HVI68652.1"
                     /translation="MNHSLGLAIESLVAILLLFTIGYCMVLNRRLQRLKADEHSLKAV
                     IAELITATEIAERAIGGLKHTVRDVNENLGSQLEAASQMSEQLKGQLSEADNVVRRLA
                     KIALAARPQANQEIASAGEATAKNVAAGDVSLRFSNAKAVAAAAQAFSERRRSGGLAA
                     "
     gene            3312..4148
                     /locus_tag="VM910_39690"
     CDS             3312..4148
                     /locus_tag="VM910_39690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HVI68653.1"
                     /translation="MKSFRNIRVIPVVVIAIASLAALKVAGLLIDGAYVFDYRLQGAS
                     SAETRGESRNESRGESKRESRGSWAQQMLNFPGGPKAEPSDVTGSVHGAPKEEAPKPA
                     APESKPRGAVIHPEDNAKTVSPSERAILERLQSRRQELETRSREIDIRENLLKAAEKR
                     IESKVEELKAIESRAAAEKEQKAEAENTRLKGLVTTYESMKPKDAAKVFDRLEMPVLI
                     EIASQIAPRKMSDIMGLMQPDAAERLTVELARRAAGDKSDGGAADLPKIEGRVLPPQK
                     SD"
     gene            4253..>5956
                     /locus_tag="VM910_39695"
     CDS             4253..>5956
                     /locus_tag="VM910_39695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI68654.1"
                     /translation="MARTAAGGFWSQIRALSRSVRRWHDGFRRSRLLAAALIVSGLSF
                     AAPCRAADAVKGEATFSAAKGYARLVLKLAEDVPSEVVTAGSIVVIRFERPVDIPVDR
                     LSDAVPDYVSSARRDPDGTAIRLSLARRVAINTMSAGERTFVDFLPDSWVGPPPSLPI
                     EVVRELAERARAAERALRQQRADALARKRPPVRVRALVQPTFVRFVFEMPDGVGASSV
                     LNDQKLTIQFNALLSFDLADAIVAAPPNVASIKQRVDGEMSTAVEIALIGDVDVHSFR
                     EDKNYLIDVAFQQPEKARALPLAEASRTQQPAQTAAASPASEKATPPPAEKPRSEEIA
                     LPTSETIAKQIKTDGTSEPPMPPPAPLDAAPSRPAQPPVTPAAEAEPAAPSAREASPS
                     APARPATETPKASSIEAKKIVSPPPSSEAKLAEAPASVDAWRDSEGLNLTFTFPSATP
                     AASFRCGDTVWLLFDSVAPFDVEPIRAKAGAIIGEVTRQQLATGQAIRMRLNRPQMHS
                     LTGDEHTGGARWSLIFADKVEAPPQPLPVMRNITDPALANVVVPLANPGRLHRLVDPD
                     AG"
BASE COUNT         1106 a         1856 c         1955 g         1039 t
ORIGIN      
        1 gatggcgttc ttgtgaagat cgccctgctg ctgcagcagg ttggaaatat tggtgtaggt
       61 ccgcatcacc tcgatcatgt ggctcatttc cgagaccacg ttgacattcg acttctcgac
      121 aaagccctgc cggaccgccg atttggtatc ggcctgggcc gcgccgtcgc cggccgcaaa
      181 caggttgtag ccctgcttga gaagtttctg cgcgtcggcg aagttgacga cgcgcatctt
      241 gccgcggatc gagtcgatac ggcttgcgcc ctccagcacc gtgatcgtgc cgtcgggcga
      301 gatgttgatg tcgtgatcgg tcgactggaa cacgatcggg ccgctctgcc cgagcacggg
      361 attgccgtca ttggtgacga gctggccctg gctgttgatc tgcaaattgc cgtcgcgggt
      421 gtagcgctcg ccgcccgccg cctgaacggc gagaaagccc gatccgtcga tggcgacatc
      481 cagcggattg ccggtgagct gcatcgcgcc tttggtgtag tcgcgatagg tgccgcgatc
      541 ctggacgtag gagacgcgcc tgtcggcgcc ctggaagttg tcctcgtgcg cgcgcgaggt
      601 cagatattcc tcgaacagca tctgatcggc cttgaagccg ttggtgttga cgttcgcgac
      661 gttgttggag atgacatcga gttgccgctc caaggttgcc tgccgcgaaa gtccgacgag
      721 aagcgtgttc tgcaccggtc ggttctcccc ttttccaaca tccgaaagca ggagccctcc
      781 caggcgcgat gctcgcggat cccttgaacc gctttggctc tcccaagccg cgcggttcgg
      841 caaggtcaga gcgaatgccg tgccaagtgg gaaagtggag cgttttgaat cggttaggcg
      901 atcggagggg gtgaattgag ggcggcagaa aggtcttgtt gaccatgttt gcccggcaac
      961 tttttcctag ctgccagcat ttcgaaaggt tccattaacc attgttacct agcgttcacg
     1021 aggtggggcg catgacgcgc tgggggcatt ggtatcgcgt gatttgaaag ctgccggccg
     1081 gctatcgggc ccgttgaaaa gagatcggac cgagcggcca tggcagacaa cgagcaggcg
     1141 gaaggcagcg ccgaaacgag cgaagtcacc gcccaaccca agagcaaatt caagctcatc
     1201 gtcatgatcg tcgccgcgct gttcgtgctc ggcgcgggtg ctgcgacctg gttcctgttc
     1261 ttccgtcatc ccgctgacga ggtgcacgcc gaggcgccgc cgccgaagcc gccggtgttc
     1321 gtcgaagtgc cggacatgat ggtcaacctc gtcaccggac cgggcgagcg cgtgcaatat
     1381 ctgaaggtga aggtcgtgct cgaggtcaag gaagaacggc aagtcgagga gatcaagccg
     1441 accatgccgc gcgtcaccga catcttccag acctatctcc gcgaactgcg cccgagcgac
     1501 ctcacgggct cggccggcct gttccgcctg aaggaggaac tgacgcggcg ggtcaacgcc
     1561 gcggtcgcgc cgaccaaggt ttcggcggtg ctgttcaagg aaatcatggt tcagtaacgg
     1621 acggatcagg acgatggcgg gcaacgatcc tgtcgaccag gatgccatag cagcacagtg
     1681 ggaagcttcg ctcggttccg aagatcccgt tgcggcggcg gaagcggctg agtcgaacga
     1741 actcacggaa tcgatggccc tgcaatgggc cgcgatggtc gaggacggca gccgcgattt
     1801 cggagccggc aagaacagcg gcgaacgcgt cctctcacag gaggaaatcg acaatctgct
     1861 cggtttcacc gtcggcgacg tcaatctcga cgaccattcc ggcatccgcg ccatcatcga
     1921 ttcggcgatg gtctcctacg agcgtctgcc gatgctcgaa atcgtattcg accgcctggt
     1981 gcggctgatg acgacgtccc tgcgcaattt cacctccgac aacgtcgagg tctcgctcga
     2041 ccgcattacc tcggtgcggt tcggcgatta catgaactcg atcccgctgc ccgccgtcat
     2101 cagcgtgttc aaggccgagg agtgggagaa cttcgggctc gctaccgtcg attccagcct
     2161 gatctattcg atcatcgacg tgctgctcgg cggccggcgc ggcaattcca cgctgcgcat
     2221 cgagggcagg ccttacacca cgatcgaaac aaatctggtc aagcgcttga tcgaagtggt
     2281 gctatcggat gccgagcagg cgttccggcc gctgtcgccg gtgacctttt cgatcgaccg
     2341 actggagacc aatccgcgct ttgccgcgat ctcgcggcca gcgaatgcgg cgatcctggt
     2401 gcggctgcgc atcgacatgg aagaccgcgg cggcaatatc gagctcctgt tgccctatgc
     2461 gaccatcgag ccgatccgca gcgtgctgct gcagatgttc atgggcgaaa agttcggccg
     2521 cgatccgatc tgggagggtc atttcgcgac cgagatcgcg caggccgaga tcgccgtcga
     2581 tgcggtgctc tatgaatcgg acattccgct gaaagagctg atgaagctca aggtcggcga
     2641 cacgctgccg ctcgacatgc gcgcggatgc gctggtgtcg gtgcgctgcg gcagcgtcac
     2701 gctcaccgag gggcggatgg ggcgggtcgg cgaccgcgtc gcgatccgcg tgacaaagcc
     2761 gttgcgcaag cccaggacca cgcttgcgat gttcgagaag gcggacgagc agaacaagct
     2821 gatggaggcc caatgaacca ttcattaggt ttggcgatcg aatcgctggt cgcgatcctg
     2881 cttttgttca ccatcggtta ctgcatggtg ctcaacaggc ggctgcagcg gctgaaggcg
     2941 gacgagcatt cgctgaaggc cgtgatcgcg gaactgatca ccgcgaccga aatcgccgag
     3001 cgcgcgatcg gcgggctcaa gcacaccgtg cgcgacgtca atgaaaatct cggcagccag
     3061 ctcgaggccg caagccagat gtcggagcag ttgaaggggc agttgtccga agccgataac
     3121 gtcgttcggc ggctggccaa gatcgcgctc gctgcgcggc cgcaagccaa ccaggagatc
     3181 gcgtccgccg gcgaggcgac cgcgaagaat gttgcggcgg gcgacgtctc gctgcggttc
     3241 tccaacgcca aggcggtggc cgcagcggca caggcgtttt ctgaacgcag aaggtccggc
     3301 ggccttgcgg catgaaatcg tttcgtaata ttcgtgtcat tcccgtcgtt gtgatcgcca
     3361 tcgcttcgct ggcggcgctg aaagtcgcgg gccttctgat cgacggcgct tacgtgttcg
     3421 actatcgcct gcagggtgcg tcgagcgccg agacgagagg cgagtcgaga aacgaatcga
     3481 gaggcgagtc gaagcgcgag tcgagaggtt cctgggccca gcaaatgctc aatttcccgg
     3541 gcggtccgaa agctgagccg agcgacgtca cgggttcggt gcacggcgcg ccgaaagagg
     3601 aggcgccgaa accggccgct ccggaaagca agccccgcgg cgcggtgata catccggaag
     3661 acaatgcaaa gaccgtctcg ccgtcggagc gcgcgatcct ggagcggctg cagtcgcgga
     3721 ggcaggagct ggaaacacgc tcgcgcgaga tcgacatccg cgagaacctg cttaaggccg
     3781 cggagaagcg catcgagtcc aaggtcgagg agctgaaggc aatcgagtcg cgcgctgcgg
     3841 ccgagaagga acagaaggcc gaggccgaga acacacgtct caagggcctc gtcaccacgt
     3901 atgagagcat gaagccgaag gatgcggcaa aagttttcga ccggctggag atgccggtcc
     3961 tgatcgagat cgcctcgcag atcgctccgc gcaagatgtc ggatatcatg gggctgatgc
     4021 agccggatgc cgccgaacgg ctcacggtcg agctcgcacg tcgcgccgcc ggcgacaagt
     4081 cagacggcgg cgcagccgat ctgccgaaga tcgaaggcag ggtcctgcca ccgcaaaagt
     4141 ccgattgaga acggcatatt taacaagtcc ttaattcggc aatcttaggg ttagcgggtg
     4201 agggtgcgtc cgcgtcatga atgcgggccg ccgaaccgga agacatgtcg gaatggcgcg
     4261 cacggctgcc ggaggatttt ggtcgcaaat ccgcgccttg tcgcggagcg tgcggcgctg
     4321 gcatgacggc tttcggcgca gccgcttgct tgcagcggcg ctcatcgtct ccggcctgag
     4381 ctttgcggcg ccctgccgcg ctgcggatgc ggtcaagggc gaggcgacat tctccgccgc
     4441 aaaaggctat gcgcggctag tcctcaagct tgccgaggac gtcccatccg aggtcgtgac
     4501 cgcaggctca atcgtcgtca tccgcttcga acgtcccgtc gacattcccg tcgatcgatt
     4561 gtcggacgcg gttcccgatt atgtgtcgtc cgcgcggcgc gatcccgacg gcacggcgat
     4621 ccgcctttcg ctggcgcgcc gcgtcgccat caacaccatg tcggccggcg aacgcacctt
     4681 cgtcgatttt ctcccggaca gttgggtcgg tccgccgccg agcctgccga tcgaggtggt
     4741 gcgggagctt gccgagcggg cgcgcgccgc cgagcgcgcg ttgcgtcagc agcgcgccga
     4801 tgcgctcgcc aggaagcgtc cgccggtccg cgtgcgcgcg ctggtgcagc cgaccttcgt
     4861 ccgcttcgtg ttcgagatgc ccgacggcgt gggcgcatcg agcgtgctga acgaccagaa
     4921 gctcacgatc cagttcaatg cgctgctcag cttcgacctt gcggatgcga tcgtcgcagc
     4981 cccgccgaac gtcgcctcga tcaagcagag ggtagatggc gagatgtcga cggccgtcga
     5041 gatcgcgctg atcggcgatg tcgacgtcca ctccttccgc gaggacaaga actacctcat
     5101 cgacgtggct ttccagcagc cggagaaggc gagggcgctg ccgcttgcgg aggcgtcgcg
     5161 cacgcagcag ccggcccaga ctgccgccgc atcgcccgca tcggagaagg ccacgccgcc
     5221 gccagccgag aagccgcgtt cggaagaaat cgccttgcca acgtcggaga cgatcgcaaa
     5281 gcagatcaag acggacggca cgtccgagcc ccccatgccg ccgcccgcac cgctcgatgc
     5341 cgcgccctcc aggccggcgc agccgccggt aacacccgca gcggaagccg agccggccgc
     5401 gccgtcggcg agggaggcgt cgccgtccgc gccggcaagg cccgcaaccg agacgccgaa
     5461 agcctcgagc atcgaagcga agaagatcgt gtctccgccg ccatcatccg aggctaaatt
     5521 ggccgaagcg cccgcgagcg tcgatgcctg gcgcgacagc gaaggcctga acctcacatt
     5581 cactttcccc tctgcaacgc ctgcggcctc gttccgctgc ggcgatacgg tgtggctgtt
     5641 gttcgattcc gttgctccgt tcgatgtcga gccgatccgc gccaaggccg gcgcgatcat
     5701 cggcgaggtg acgcgccagc agcttgccac cggacaggcg atccgcatgc gcctcaaccg
     5761 cccacagatg cactcgctga cgggcgacga gcacactggc ggcgccagat ggagcctgat
     5821 cttcgccgac aaggtggaag cgccgccgca accgcttccg gtgatgcgca acatcaccga
     5881 ccccgcgctc gccaatgtcg tggtgccgct ggccaatccc gggcgtctgc atcggcttgt
     5941 cgatcccgac gccggc
//
LOCUS       DATLJT010001749         4013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_431292,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001749 DATLJT010000000
VERSION     DATLJT010001749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431292"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..259)
                     /locus_tag="VM910_39700"
                     /pseudo
     CDS             complement(143..259)
                     /locus_tag="VM910_39700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            608..844
                     /locus_tag="VM910_39705"
     CDS             608..844
                     /locus_tag="VM910_39705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68655.1"
                     /translation="MPVRRTECRIKRAGHLLGPSQQLDLFGGDRPRAIVDMPAWPELP
                     AETRTTLTGLMVRLILEHAGANRPGSPREAGHDL"
     gene            834..2897
                     /locus_tag="VM910_39710"
     CDS             834..2897
                     /locus_tag="VM910_39710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVI68656.1"
                     /translation="MISDKVRPHHLERKALLYVRQSSAHQVLHNRESSALQYAMRDRL
                     AALGWSEIEVIDDDLGRSAAGGVQRAGFERMVAEVCLGKVGAVCAREVSRFARNSRDW
                     QQLIEMCRVVDTVLIDQETIYAPRHGNDRLLLGLKGSLNEYELDLLRQRSLSARYEKA
                     RRGELVVGAPVGFVKAGDHYEKDPDRRVQEAITLVFDKVLELGSARQALLWFHEHDLD
                     LPVRGKDGTTLWRRPNYATIHRMIENPVYGGAYAYGKTAVATGHGVAGVKARRKARAD
                     WLALMPNAHEGYISWEKAETIRKMVSGNVPTSRHHGAPKHGDALLSGLIRCRRCGRKL
                     TLRYSGARHDIPRYSCTRGWMDNGEPRCIAFGGLQVDDAVADALLTVVGPGAVAAAVS
                     AEAEAIQRRDQVREALERDLEAARFAADRAFRQYDAADPANRLVTGELEARWNKALAH
                     AAEVEGKMTAHDATAPTPAADPASLVALAADLKSVWTAPSTDARLRKRIVRTVIHEVI
                     ADIDQEAAEVLLVVHWIGGAHSEIRLPRRRRGQRNNTSTDVIAAVRQLVLVAHDDLIA
                     GILNRNGLVTGNGNRWTRERVTSLRSHHRIPVHKPAEDEVEPWLQLNKAAQFLHIAAK
                     TLRRAAEAGEILGIHPLPDGPWIFSRTDLSTPAAQTITERARRNPRRPAGPDPDHQSL
                     FPSMT"
     gene            complement(2921..3851)
                     /locus_tag="VM910_39715"
                     /pseudo
     CDS             complement(2921..3851)
                     /locus_tag="VM910_39715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          684 a         1270 c         1314 g          745 t
ORIGIN      
        1 gagagcgctg accgggtact gaaggaatgc gcgatgcgtc ggcgccagct gaagtggctg
       61 tggcgccggc tcaactgtat tagctaagac ttgatctgac ggtcgggctg gatgtctgaa
      121 ccggtgtatc tgtcgatcgt agtcaagctg ccatgagttt ctccatcgcc aaggggaggg
      181 tgtcaagaaa ggtttgcatc ggcgtcttgc cgaagcacca gcggccttga tgcggtcgtc
      241 gataattgta caacatcatt tgagaacgcc cacgggacga cgttccgtga gcttttgtat
      301 ccttggcatc cgtggttcgc gcgtcgcgtc gccgtgcatg aggcgatcgg gaagtcgaac
      361 gatcttgttt tccgctgcac gctgagcggt tctgaggccg gccgctcggc ggagatcccg
      421 gcttggatgt ttgaccgggc ggcctgtgcc gaggcgcggc ttactgccgc cccctatgtc
      481 agtgcggcgg ccttgtcggc gttgcgtgat ctgctgcagc aggcgctgaa ggatagatcc
      541 gtttcatcga gcgtgccggt ttcgggcgta tcagggattt ctcgcgacca gaatcgaaga
      601 gaagcccatg cctgtcagga gaacggagtg ccggatcaag cgggcgggtc atctgctggg
      661 cccctcgcag cagctcgacc tgttcggtgg cgatcgcccc agggccatag tggatatgcc
      721 agcgtggccg gaactgccgg cggagacacg gacaacgctg accggtctga tggtgcggct
      781 gatcctggag catgccggcg cgaaccggcc cggatcgccg cgggaggccg gtcatgatct
      841 ctgacaaggt tcgtccccat catctggaac gcaaggcgtt gctttacgtc cggcaatctt
      901 cggcccatca ggttctgcac aaccgtgaga gtagcgcgct gcaatatgcc atgcgggatc
      961 gcctggcagc actcggctgg tccgagatcg aggtgatcga cgacgatctg ggccgttcgg
     1021 ccgcaggcgg cgtccaacgc gccggattcg agcgaatggt cgcggaagtg tgtctcggca
     1081 aggtcggcgc cgtgtgtgcc cgcgaggtct cgcgcttcgc ccgcaacagc cgggactggc
     1141 agcagctgat cgagatgtgc cgcgttgtcg atactgtgct gatcgatcag gagacgatct
     1201 atgcgccccg gcacggtaat gaccggctgc tcctcgggct gaagggaagc ctcaacgaat
     1261 acgaactcga tctgctgcgc cagcgttcgc tctcggcccg ctacgagaag gcgcgtcgtg
     1321 gcgagcttgt cgtcggcgcg ccggtgggct tcgtcaaggc cggcgatcac tacgagaagg
     1381 atcccgatcg ccgcgtgcag gaggcgatta cgctggtctt cgacaaggtg ctggaactcg
     1441 gcagcgcacg ccaagctcta ctctggttcc atgagcatga tctcgacctt ccggtgaggg
     1501 gcaaggacgg caccacgctt tggcggcgac cgaattatgc caccatccat cggatgatcg
     1561 agaaccccgt ctacggcggc gcctatgctt atggtaagac ggccgtggcg acaggccacg
     1621 gcgtcgccgg tgtgaaggcg cggcggaagg cacgtgccga ttggctggcg ctcatgccga
     1681 acgctcatga aggctatatc agctgggaaa aggccgagac gattcgcaag atggtcagcg
     1741 gcaacgtgcc aaccagccgc catcatggcg cgcccaagca tggcgatgcc cttttatcag
     1801 ggctcatccg ctgccgccgc tgcggtcgga agctgacgct ccgctattcg ggtgcaaggc
     1861 acgatattcc gcgctacagc tgcacccgcg gctggatgga taacggcgag ccgcgctgca
     1921 tcgcctttgg cggtctgcaa gtcgacgacg ccgtcgcgga tgcgcttctc accgtcgtcg
     1981 gccccggcgc cgttgcggcc gctgtttcgg cagaggcgga agccatccag cggcgggacc
     2041 aagtacgcga ggcgctcgag cgcgatcttg aagcggcgcg ctttgccgct gaccgggctt
     2101 tccgtcaata cgacgcggcc gatccggcca accggcttgt taccggcgaa ttggaggcac
     2161 gctggaacaa ggcgctcgcg catgcagcgg aagtcgaagg caaaatgacc gctcatgacg
     2221 caactgcgcc gacacccgcc gccgatccag cctcactcgt cgcgctcgca gccgacctga
     2281 agagcgtgtg gaccgctccc tccacggatg ctcggctgag gaagcgcatc gtgcgcaccg
     2341 tcatccacga ggtgatcgcc gatatcgacc aggaggcggc agaggtcttg cttgttgtcc
     2401 attggatcgg cggtgcccac agcgagatac gtctgcccag gcgccggcgc ggccagcgca
     2461 acaacacgtc gaccgacgta atcgccgcgg tccgccaact tgtgctggtc gcccatgacg
     2521 atctcatcgc cggcatcctg aacaggaatg gcctcgtcac gggcaatggc aatcgctgga
     2581 cccgcgaacg ggtgacgtcg ctgcgctcgc atcaccgcat ccccgtacat aagccggcgg
     2641 aagacgaggt cgaaccctgg ttgcaattga acaaggccgc gcaatttctc cacatcgccg
     2701 ccaagacact ccggcgcgcg gcagaagccg gcgagatcct cgggatccat cccttgcccg
     2761 atggtccgtg gatcttcagc cgcaccgatc tctccacccc ggcagcccaa accatcacgg
     2821 aacgggcgcg gcggaacccg agacgccccg cgggaccgga tcccgatcac caaagcctat
     2881 ttccttcaat gacatagaca gatgggtgtt ctgatgcgcg gttgtactcg gcgagccagg
     2941 cgtcgaggtc agcctgcagc tcgtcgatgg tgcgatagat cttcttgcgg aaggccacgc
     3001 ggtagaactc gtcgagcacg gtgcggtgga agcgctcaca gatgccgttg gtctgcgggc
     3061 tcttggtctt ggtgcggctg tgatcgatgt cctcgaccgc caggtagagc tcgtattcgt
     3121 ggctctgatt gccgcagaac tcggtgccgc gatcggtgag catgcgcgac agccggatgc
     3181 catgctcgtc gtagaacggc accacccggt cgttgaggat ctcggccgcg gtgatcgggg
     3241 tcttgcggtc gtagagcttg gcgaaggcga ccttggcgta ggtgtcgatg acggtctgct
     3301 gatagatccg ccccaccccc ttcaaggtgc cgacatagaa ggtgtcctgg gccccgcaat
     3361 agcccgggca ttcgctctcg aactcgccgt gcgcttcctt gtccgccttg gccttttcga
     3421 gggcgacgac ctgtgcctcg gtgagcaaga tgccgtcctg cgccaccttg gcctcgagcg
     3481 ccttcaggcg cagcttcatc gaggtcagct cgtggcgctg ccacacgcag cgcacccccg
     3541 ccggcgagat cgacaggccc tgccgcttca gcgcctccga gatgcgcacc tggccccagg
     3601 ccggctgctc gattggcgag cgccacgacc gcctcctcga tcgcgatcgg cacgcggttt
     3661 ttcaggatcg gcttgcggcg gctgatctcc tggagcgcct gctcgccgcc cttatcgtag
     3721 agctccttga agcggtagaa gctgtcgcgg ctgtagccca tcatcttaca ggcctggcta
     3781 acattgccga gctgcttggc caattccagc aacccgacct tggcgcggat aatcttctgc
     3841 tcttgtgtca tcgtggtctc cgtcacgccg gaggggcggg ctgcccgcgg ccgcgttcgg
     3901 cgggcgggcc caggtcatgg acctgctcgc cccgctcggg ccggtctacc aggcgggcac
     3961 gctgtcgggg aacccgctgg ccaccgcggc cggcctggcc acgctgcgcg cct
//
LOCUS       DATLJT010001750         1695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2732214,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001750 DATLJT010000000
VERSION     DATLJT010001750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732214"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VM910_39720"
     CDS             complement(<1..405)
                     /locus_tag="VM910_39720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVI68657.1"
                     /translation="MADPVMAAAQSSPDVVVRRSRMDRFIDGIELLAAFFVGIVAADI
                     FTSVLLRYFFSVQIPDSYDFGRLLLGILIFWGIAATSYRGTHITVDLVYANVGPKLQR
                     FIDVFATLVLLFVVSVQTWTLFDKVVSTRADNV"
     gene            complement(561..1601)
                     /locus_tag="VM910_39725"
     CDS             complement(561..1601)
                     /locus_tag="VM910_39725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI68658.1"
                     /translation="MPAHRTKRPITLIVAAAALSVATSAALAQDKTFELKLSHWVPPT
                     HPLQKAMEEWGASVEKASNGTIKSKVFPSQQLGKAFDHYDMARDGIADFTYVNPGYQP
                     GRFPIISAGELPFLVGDAKGGIRAIDAWYRKYAPTEMKDVKYCFSFILDPLTWHSSKK
                     KIVVPGDIKGMKVRPSQATVAAWVTLLGGTNVQASATEVRDVMEKGVAEAVTFPWGSV
                     PLLGVDKVTKYHMEAPLHTVTFQWLMSPRTYAAMSPAQKKVIDDHCTTEWAGKFADPW
                     ADFEHAGLAKVKAEPGHEVYTITDAQLDEWKKSAEPLEKQWTDNVRKAGGDPDTIMKE
                     LKQSLAQYKAAY"
BASE COUNT          292 a          520 c          551 g          332 t
ORIGIN      
        1 cacgttgtcg gcccgcgtgc tcacgacctt gtcgaacaag gtccaggtct gaaccgagac
       61 gacgaacagc aggaccagcg tcgcgaacac gtcgatgaag cgctgtagtt tcggcccgac
      121 attggcatag acgaggtcga ccgtgatgtg agtgccccgg tacgaggtgg cggcgatgcc
      181 ccagaagatc aggatgccca gaaggagtcg tccgaagtcg tagctgtcgg gaatctggac
      241 gctgaagaag tatcgcagca ggaccgaggt gaagatatcg gcggcgacga tgccgacgaa
      301 gaacgcggcc agcagctcga tcccatcgat gaagcgatcc atgcggctgc gcctgaccac
      361 gacgtccggg ctcgattggg ctgcggccat gacgggatca gccattcctc aacgtctgcg
      421 ccgatgagca tgcgcgagag cgggcgtcga ctgcaagcgt cgctcgggcg ggtgggcgtc
      481 tccgcaagca gcggcaaagc gcgcctgccg ctgttttcat cgcgcgacgg cttgcgcgac
      541 tgaggcgggg aagatgccgg tcagtacgcc gccttgtatt gggcgagcga ctgcttgagc
      601 tccttcatga tggtatccgg atcgccgccg gccttgcgga cgttgtcggt ccactgcttc
      661 tccagcggtt ccgccgactt cttccattcg tcgagctgcg cgtcagtgat cgtgtagacc
      721 tcgtgcccgg gctcggcttt caccttggcc agtccggcgt gctcgaaatc cgcccaggga
      781 tcggcgaatt tacccgccca ctcagtggtg cagtggtcgt cgatgacctt cttctgtgcg
      841 ggcgacatgg cggcataggt gcgcgggctc atcagccact ggaacgtgac ggtgtggagc
      901 ggcgcctcca tgtgatactt cgtgaccttg tcgacgccca gcagcggcac cgagccccaa
      961 gggaaggtga ccgcctccgc cacgcctttc tccatcacgt cgcgcacctc ggtcgcgctc
     1021 gcctgcacgt tggtgccgcc gagcagcgtc acccaggccg ccacggttgc ttgcgacggg
     1081 cgcaccttca tgcccttgat gtcacccggc accacgattt tcttcttgct cgagtgccac
     1141 gtgagcggat cgaggatgaa ggagaagcaa tatttcacgt ccttcatctc ggttggcgcg
     1201 tatttgcgat accaggcgtc gatcgcgcgg atgccgccct tggcgtcgcc caccaggaac
     1261 ggcaattcgc cagcggagat gatggggaag cggccgggct gatagcccgg attgacataa
     1321 gtaaagtcgg cgatgccgtc gcgcgccatg tcgtaatggt cgaaggcctt gccgagctgc
     1381 tgggacggaa acactttcga cttgatggtg ccgttcgacg ccttttcgac cgacgcgccc
     1441 cattcctcca tcgccttctg cagcggatgc gtgggcggca cccagtgcga aagcttgagc
     1501 tcgaaggtct tgtcctgagc gagcgcggcg gatgttgcga ccgacagcgc tgctgctgcg
     1561 acgatcaatg tgatgggacg cttggttcga tgcgctggca tcgtcttcct cctcgccgga
     1621 acttgtggcc gttgcgccgc cttgtcggca ttgagcccga agcgatcggc actgtaaagt
     1681 gacgttcgcg ggcct
//
LOCUS       DATLJT010001751         1548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3651689,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001751 DATLJT010000000
VERSION     DATLJT010001751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651689"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VM910_39730"
     CDS             complement(<1..206)
                     /locus_tag="VM910_39730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HVI68659.1"
                     /translation="MSDFALPVVLGIALAAATGFRVFLPMLIVSAAAYTGHLSLDNSF
                     AWLGTRSALAMLSVAAVAEILAYY"
     gene            complement(358..1336)
                     /locus_tag="VM910_39735"
                     /pseudo
     CDS             complement(358..1336)
                     /locus_tag="VM910_39735"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1333..>1548)
                     /locus_tag="VM910_39740"
     CDS             complement(1333..>1548)
                     /locus_tag="VM910_39740"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HVI68660.1"
                     /translation="AGFLMSYGPNLPDLYRRAAEFADKILRGAKPADIPVEQPTKFEL
                     VVNLKSAKTLGLTIPGTVLSRADEVIE"
BASE COUNT          327 a          458 c          459 g          304 t
ORIGIN      
        1 atgtagtaag ctaagatttc tgcgactgct gccacactga gcattgccag tgcggatcgc
       61 gtgccgagcc atgcgaagct attgtccaaa gagaggtgtc cagtataggc cgcggcgctc
      121 acaatcagca tcggcaaaaa cactcgaaag ccggtcgcag cggctagcgc gatgccgaga
      181 acaactggta atgcgaagtc cgacatgcag ctgctctcgg gatttcgatg gaccgctagg
      241 cccttctcga gcgacattgc ggtgcatccc ggatgatgtc cacctatgcc tcaaaagcgg
      301 acatcgttca gcacgatcgc gacgtccgct ttgcgtcaaa agcggacatt ggacgggcta
      361 ttcaatcacc tcgtcggcca gctgttgcag atacaggggc acatcgaggc cgagcgcctt
      421 cgcggtcttg aggtttatca ccagctcgaa tttggtgggc gcctgaaccg gcagctcact
      481 tggtttctcg cccttgagca cgcggtcgac atacgacgcc gcacgccggt actcgtcggg
      541 aatattgggg ccgtaagaga tcaggccgcc gatttcccca atagatggtg gggacacgcc
      601 agcgagcagc tagcccaata atttctgcac catgactaat tgtgaagacg tccggtatga
      661 cgatgaagcc actattaggc tcgcgtgcct gtgtcgtaaa gagcgcttcg agctcggaca
      721 tgtccttaac ggggccgaca atcgcctcca cgccgtggga cgcggcggca gttctgaacg
      781 ggttcagata atctttgaca aacggtgctg taggcgggtt gaacactaac gtgactctgc
      841 taacgcgcgg cgcaatatct ctgagtacct ccacccactt gccgcccagc gaccccacga
      901 taggagtgaa accagtcaca ttgccgccag gttgcgcgag gctcgtgacg aaaccgctac
      961 cgatgggatc gccaacctcg acgaaaatca ccgggatggt acgcgtctgc tgaagtaccg
     1021 ccgcggtagc gggtgtggaa ctcgtaacaa tgaggtccgg ttggagcgcg acgagttcct
     1081 tcgcgaatcg tttgattgac tcggcatcac ctccagccgc gtgaatctcc atctcgatgt
     1141 tgcggccctc cgtccacccg agttttcgaa gttcctcccg gaacagcgcg agcccggatt
     1201 gtgcgtcacg gtcgctctcg gcaaacccca acaacacgcc aacgagtcgc gatcgggtcg
     1261 gctgctgcgc atatgcagcg ggcggtcgga tgaccgtggc agcggcaagc agcgtgatga
     1321 attcgcgccg cctcattcga tcacctcgtc ggcgcgggag agcaccgtcc cgggaatggt
     1381 aagaccgagc gtcttggcgc tcttcaagtt gacgacaagc tcgaatttgg tcggctgctc
     1441 gaccggaatg tcggccggct tcgccccgcg caaaatcttg tcggcgaact cagcggcgcg
     1501 acggtacagg tcgggcaggt ttggtccata ggacatcagg aatcccgc
//
LOCUS       DATLJT010001752         1577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1581053,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001752 DATLJT010000000
VERSION     DATLJT010001752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581053"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VM910_39745"
     CDS             <1..683
                     /locus_tag="VM910_39745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HVI68661.1"
                     /translation="VFIKIPGTPEGLPAIEEAIFAGVPINVTLLFSDDQYLAAANAFM
                     RGIERRIEAGLTPDVRSVASIFISRWDAAVTGKVPKALNDRLGIAIAKRAYKAYLALL
                     SSPRWMRAYNAGARPQRLLWASTGTKDPEASDVLYVKSLAAPLTVNTMPEGTLKALAD
                     HGDVGATLPADGGNSEEELARFAEAKIDVHALSRQLQDEGAKSFVKSWNDLMSVISSK
                     SAVLKRAS"
     gene            775..1461
                     /locus_tag="VM910_39750"
     CDS             775..1461
                     /locus_tag="VM910_39750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVI68662.1"
                     /translation="MLVVDVGGTSVKILATGQKEERSFPSGPTLTPKQMVSGVKKLAR
                     GWTYDVVSIGYPGPVLHCRPMAEPYNLGRGWVGFDFAAAFGRPVKVVNDAAMQALGGY
                     NGGKMLFLGLGTGLGSTMIVNGIVEPMELGHLPYKKGTYEDYVGRVGLERYGKKKWRR
                     HVAEVVKRFIAALEPDETVIGGGNVKKLHPLPPHSRAGDNANAFRGEFRLWGEERALP
                     HTGTRSKPRT"
BASE COUNT          308 a          494 c          522 g          253 t
ORIGIN      
        1 atgtcttcat caagatcccg ggcacccccg aaggcctgcc ggccatcgaa gaggcgatct
       61 ttgcgggggt gccgatcaac gtcacgctgc tgttctccga cgaccagtac ctggcggccg
      121 ccaacgcatt catgcgcggc atcgaacggc gcatcgaagc gggattgacg cccgatgtgc
      181 gctcggtggc gtcgatcttc atcagccgct gggacgccgc ggtcaccggc aaggtgccga
      241 aggcgctcaa cgatcggctc ggtatcgcca tcgccaaacg cgcctacaag gcctatctcg
      301 cgttgctgag ttcgccacgg tggatgcgcg cctacaatgc cggcgcgcgc ccgcagcgcc
      361 tgctgtgggc cagcaccggg accaaggatc cggaggcttc ggatgtcctc tacgtcaaga
      421 gcctggccgc gccattgacg gtgaacacca tgcccgaggg cacgctgaaa gccctcgccg
      481 accacggcga cgtcggcgca acgctccctg cggacggcgg caactccgaa gaggagctgg
      541 cacggttcgc cgaggcgaag atcgatgtcc atgcgttgtc gcgacagctt caggacgaag
      601 gtgccaagtc attcgtcaag tcgtggaacg acctgatgag tgtgatcagc tccaaatccg
      661 ccgtgctcaa gcgagcgagc tagcgcggtg aaggcgaagt cgcatcccgc cgccgagcgc
      721 cggcgagggc ggcaagatcg agctgacgtc gcaaagacgt ccgcagccgg gaaggtgttg
      781 gtggtcgatg tgggcggaac ctcggtcaag atccttgcca ccggacaaaa agaggagcgc
      841 tcatttccat ccgggccgac gttgaccccc aagcaaatgg tgtccggggt caagaagctc
      901 gcccggggtt ggacgtatga cgtggtgtcg atcggctatc ccggcccggt tctccactgc
      961 cggccaatgg ccgaaccata caacctggga cgcggatggg tcgggttcga tttcgcagca
     1021 gcgttcggtc gccctgtgaa agtcgtcaac gacgccgcga tgcaggctct ggggggctac
     1081 aacggcggca agatgctctt tctaggcctc gggacgggac ttggatcgac catgattgtg
     1141 aacgggatcg tggagcccat ggagctcggt cacctgcctt acaagaaggg tacctatgag
     1201 gactacgtgg gccgggtcgg gctggagcgt tacggcaaga agaaatggcg ccgtcacgtt
     1261 gccgaggtgg tgaagcgctt catcgccgcg ctcgagcccg acgaaaccgt gattgggggc
     1321 ggcaacgtca agaagctcca cccgctgccg ccgcattctc gcgcgggcga caacgccaac
     1381 gccttccgcg gcgaattccg cctgtgggga gaagaacgcg ctctcccgca caccgggaca
     1441 cgctcgaaac cacggacttg atcggagagg tgccacagat ggctgcacga tcggagatgc
     1501 ctcgcccgcg atcgacgagc agacccgccg catggcgtgc gctcgaagat cactacggga
     1561 caatgcgtgg ccgccac
//
LOCUS       DATLJT010001753         4822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3190903,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001753 DATLJT010000000
VERSION     DATLJT010001753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190903"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..172
                     /locus_tag="VM910_39755"
     CDS             20..172
                     /locus_tag="VM910_39755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HVI68663.1"
                     /translation="MLLSLIRMIQAFRDYQRNVAELSQLSDRELADIGLDRSDIPRVA
                     AGNYNG"
     gene            296..1729
                     /gene="trmFO"
                     /locus_tag="VM910_39760"
     CDS             296..1729
                     /gene="trmFO"
                     /locus_tag="VM910_39760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HVI68664.1"
                     /translation="MIGTKSSTGIVHVVGAGLAGSEAAWQAAEAGCRVVLHEMRPERM
                     TEAHRTDGCAELVCSNSFRSDDAANNAVGLLHAEMRRLYSLIMRAADANQVPAGGALA
                     VDREGFSAAVTRALQEHPRIEIRRGEITGLPSADWDNVIVATGPLTSAPLAEAIRELT
                     DEDALAFFDAIAPIVHRDSIDLSIAWFQSRYDKSGPGGTGADYINCPMTEEQYDAFVD
                     ALLAGEKTDFKDWEANTPYFDGCLPVEVMAERGHETLRHGPMKPVGLTNRHNPSQKPY
                     AIVQLRQDNKLGTLYNMVGFQTKLKYGAQQHIFRAIPGLENAEFARLGGLHRNTFLNS
                     PKLLDAQLRLRADRRLRFAGQMTGCEGYVESASIGLIAGLQAAADLQGRELSPPPPTT
                     ALGALLGHITGGHIETIEHGSRSFQPMNINFGLFPPLAQAPTKKADGTRLRGNEKTVA
                     KKQALSTRALSDLDRWIAEHLRVPAAA"
     gene            1737..2840
                     /locus_tag="VM910_39765"
     CDS             1737..2840
                     /locus_tag="VM910_39765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HVI68665.1"
                     /translation="MRLPKDDADTLSARWIDGVLLKRDVFSTVERGRFRAGPSEIDAV
                     LRRLDQVPWWSYTIARHLFTRERRALHLAKDIRVGPDLLWAGRRALVRGFIDGVALHL
                     AKPYGDVAYFRSAKAALRRLHRAGICHNDLAKEQNWLRGADGRAYLTDFQLAACFKKR
                     SRLFRIAAYEDLRHLLKHKRSYVPEALTPKERKILANKSLVASVWLATGKKVYRAITR
                     GLFNFTDREGGGRRLVNDAPVLIDLIRRNPAVRDTAIVAFADRRTGVGLYAFVEADKG
                     GLEAQLRNEIAAARGPKPPEHIQVVHALPRDASGKPRTEILQLVAMNQLDLIEPMMAN
                     EADREFLKDILEQRKNLRDRFNFEAHDMTLPSP"
     gene            complement(2861..3598)
                     /locus_tag="VM910_39770"
     CDS             complement(2861..3598)
                     /locus_tag="VM910_39770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68666.1"
                     /translation="MALSLSIKALDAGYGAVNALRGVSLDVVGGETVALLGTNGNGKS
                     TLMKCVMGLVRPRRGSMVLTVEGTSHDLTRLSPEAIVDLGVALVPEGRRLFPKLTVVE
                     NLMLGAFRKAARRTIEHNLTMAFETFPVLKERKGQIAGTLSGGQQQMLAIARALMSSP
                     RLLLVDEPSVGLSPLLVSQTITKIGELKKSFGLTVLMAEQNFNQAIRIADRGYVIVHG
                     EIAVSAASVAELQANDIVKRLYLGGVA"
     gene            complement(3588..4331)
                     /locus_tag="VM910_39775"
     CDS             complement(3588..4331)
                     /locus_tag="VM910_39775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68667.1"
                     /translation="MTAPGEAPLLRIEALTKSFGGFVALDNINVDIGKGERFGLIGPN
                     GSGKTTLVNCISGALRPSKGSVLFGGEDITTLPPNQRSHRGIARSFQIPRPFRSMTVI
                     ENLMVALDFAASRHLLESASHRRDAAMAVLTRIGLASKADVSTAALTQVELRKMELAR
                     AMAIGPKLLISDEAMAGLSTSEVDEVLDLLISLGGEDIAIIMIEHIMQAVMRFSERVM
                     CLDAGRIIAIGSPSEVMGNPRVQEAYLGT"
     gene            complement(4328..>4822)
                     /locus_tag="VM910_39780"
     CDS             complement(4328..>4822)
                     /locus_tag="VM910_39780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI68668.1"
                     /translation="YVKMLFFVQALLVVVAVATTRTIQNSRIGRGLQALRDDELAAEC
                     TGVPTLRLKLLACVISGALISAGGAPAAMYLQFANPESAFNLNYSVSVLAMSLIGGTA
                     HWIGPVLGAVLLGATQQLLAVTISSEVNVLVLGIMLVVFVVGAPKGLIGLLRRAPSRI
                     GDRK"
BASE COUNT          961 a         1534 c         1451 g          876 t
ORIGIN      
        1 attctgcaag gagactacca tgttgctctc gctcatccgc atgatccagg cgttccggga
       61 ctatcagcgc aatgtcgctg agctgtccca gctcagcgat cgcgagctgg ccgacatcgg
      121 cctcgatcgc tcggacattc cgcgcgtagc agccggcaac tacaacggct gattccgttt
      181 cagccgactg actacggata gcgcccgcct cgctgcgggc gctatcgttt tatggggcct
      241 tcacgggaca gcacgcaggt gcgtttcggc taaaaacgcg ctaaccctgc gccccatgat
      301 cggaactaaa tcttccactg gaatcgttca tgtcgtgggc gccggcctcg cgggttcgga
      361 ggccgcctgg caggctgcag aagccggctg ccgcgtggtt ctgcacgaga tgcgaccgga
      421 gcgtatgacc gaggcgcatc gtacagacgg ctgtgccgaa ctcgtatgtt cgaactcgtt
      481 ccgctcggac gacgcggcca acaacgccgt agggctcctc cacgccgaga tgcgccggct
      541 ttactcactc atcatgcgcg cggccgacgc taaccaggtc ccggcgggcg gtgccctcgc
      601 ggtcgaccgt gaaggttttt ccgctgccgt aaccagagcg ctgcaggagc atccgcgaat
      661 cgagatccgg cgcggcgaaa ttactggcct gccatccgcc gactgggaca atgtcatcgt
      721 cgcgaccggt cctctcacct ctgctccgct cgccgaggcg attcgtgagc taacggacga
      781 agacgcgctg gcgttcttcg atgcgattgc cccgatcgtg cacagggact caatcgacct
      841 gtcgattgcc tggttccagt cacgctacga caaatctggc cccggcggca ccggcgccga
      901 ctacatcaac tgtcccatga cggaggagca atacgacgcc tttgtcgatg cgttgctggc
      961 tggcgaaaaa accgatttca aggactggga ggccaacacg ccctatttcg acggctgcct
     1021 gccggtcgag gtcatggccg agcgcggtca tgagacgctg cggcacggac cgatgaaacc
     1081 tgtgggcctg accaatcggc acaatccctc gcaaaagcct tatgccatcg tgcaactgcg
     1141 gcaggacaac aagctgggca cgctctacaa catggtcggt tttcaaacca aactgaaata
     1201 cggtgcgcag cagcacattt tccgcgccat cccgggactt gaaaacgccg agtttgcaag
     1261 gctcggaggg ttacaccgca acacttttct gaactcgcca aagctgctgg atgcccagtt
     1321 gcggttgcgc gccgaccggc ggctgcgttt cgccggccag atgacgggct gcgaaggcta
     1381 tgtcgaatcg gcaagtattg gattgatcgc cggcctccag gccgcagccg acctacaggg
     1441 aagagagctt tcgcccccac ccccgaccac agcgctgggg gcactgctcg gccatatcac
     1501 cggtggccac atcgagacca tcgaacacgg cagccgctca ttccagccga tgaacatcaa
     1561 tttcggtctg ttcccgccgc tcgcccaggc gccgacgaaa aaggccgacg gcaccaggtt
     1621 gcggggcaat gaaaagacgg tcgcgaagaa acaggcgctc agcactcgcg ccttgtcgga
     1681 tctggaccgc tggatcgccg agcatctccg cgtgccggcg gcagcgtgag cgtctcatga
     1741 gattgcccaa ggacgatgcc gataccttgt cggcgcgctg gatcgatggc gtgttgctta
     1801 agcgcgacgt attttcgacc gtggagcgcg gtcggtttcg cgcgggtccg agcgaaatcg
     1861 atgccgtcct gcgccgactc gatcaggtgc catggtggtc ctacactatt gcgcgccacc
     1921 tgtttacccg ggagcgtcga gctctgcatc ttgcaaaaga catccgcgtc ggccccgacc
     1981 tcttgtgggc cggacggcgc gcgctggtgc gtggcttcat cgatggcgtc gcactgcatt
     2041 tggcgaagcc gtacggcgac gttgcctatt tccgctcggc caaagccgcc cttcgtcgat
     2101 tgcatcgcgc aggaatctgc cacaacgacc tcgccaagga gcagaactgg ctgcgcggcg
     2161 ccgacggacg cgcctatctt acggactttc agctcgccgc atgcttcaaa aagcgttccc
     2221 ggctgttccg tatcgccgcc tatgaggatc ttcgacacct gttgaagcac aagcgcagct
     2281 atgtgccgga ggcgctgaca ccgaaagagc gcaagatcct cgccaacaaa tcgctggtcg
     2341 cgagtgtctg gcttgcgacg ggcaagaagg tctatcgcgc gatcacgcgc gggttgttca
     2401 acttcaccga tcgcgaaggc ggcggacgac ggcttgtcaa tgatgcgccg gtgctgatcg
     2461 atctgatccg caggaatcca gcggtccgcg acaccgccat cgtcgccttt gccgatcggc
     2521 gcaccggggt cggcctctac gcgtttgtcg aggccgacaa gggcgggctg gaagcgcaac
     2581 tgcgcaacga aatcgccgca gcgaggggtc cgaagccgcc tgaacatatc caggtggttc
     2641 atgccttgcc gcgcgatgcc agcggcaaac cgcgcaccga aatcctgcag cttgtggcga
     2701 tgaaccagct cgacctgatc gagccgatga tggccaacga ggccgatcgg gagtttctga
     2761 aggacattct ggagcaacgg aagaacttgc gcgatcgctt caatttcgaa gcccatgaca
     2821 tgacgttacc gtcgccctga cggccaagat ctagcccgct tcaggcgacg ccgccgagat
     2881 agagccgttt gactatgtcg ttggcttgca actccgcgac ggaggcagcc gaaaccgcga
     2941 tttcgccgtg cacgatcaca tagccgcggt cggcgatgcg gatcgcctgg ttgaagttct
     3001 gctcggccat cagcacggtc aggccaaaac tctttttcag ttctccaatc tttgtaatgg
     3061 tttgcgacac caggagcggc gacaggccga ccgaaggctc atcaaccaga agcaggcgcg
     3121 gcgaggacat caacgcacgg gcgatggcga gcatttgctg ctgtccgccg gacaaggtgc
     3181 ccgcgatctg gcccttccgc tccttcagca cgggaaaggt ttcgaaggcc atcgtgaggt
     3241 tgtgttcgat ggtccgccgt gcggccttgc ggaaggcacc caacatcaag ttttccacga
     3301 ccgtcagctt gggaaacagc cgccgccctt cgggaacgag agccacaccg agatcaacga
     3361 tcgcctcggg cgaaagccgg gtcaggtcgt gcgatgttcc ttcgaccgtc agcaccatgc
     3421 tgccgcgccg tggacggacg agccccatga cgcacttcat caacgtactc ttgccgttcc
     3481 cgttggtacc gagcagtgcg acggtctcac ccccgaccac gtcgagcgag acaccgcgca
     3541 gcgcgttcac cgcgccgtaa cctgcatcga gcgctttgat cgagaggcta agtgccaaga
     3601 taggcctcct ggacacgtgg attgcccatg acttcggacg gcgatccgat cgcgatgatc
     3661 cggccggcgt cgaggcacat cacgcgctcc gagaaacgca tcacagcctg catgatgtgc
     3721 tcgatcatga tgatggcgat atcctcgcca ccaaggctga tcagaagatc cagcacttcg
     3781 tcgacctcgg aggtggacag acccgccatc gcctcatcgg aaatcaggag cttcggacct
     3841 attgccatgg cgcgagcgag ctccatcttg cgcaattcca cctgcgttag cgccgccgtc
     3901 gagacgtcgg ccttggatgc aagaccaatg cgcgtcagga ccgccatcgc ggcatcgcgc
     3961 cggtgggacg ccgattccag gagatggcga ctggcagcga agtcgagcgc caccatcaga
     4021 ttttcgatca ccgtcatgct tctaaacggc cgcggaatct gaaagctccg cgcgatgccg
     4081 cgatgcgagc gctggttcgg cggcaatgtc gtgatatcct cgccgccaaa cagcacggac
     4141 cccttgctcg gacgcagcgc gccggaaatg cagttgacca gcgttgtctt tcccgaaccg
     4201 ttcggaccga tcaggccgaa gcgctcacct ttgccaatat cgacattgat gttgtcgagt
     4261 gcgacgaagc cgccaaaact tttggtcaag gcctcgatcc gcagcaatgg agcctctccg
     4321 ggcgccgtca tttgcgatct ccgatccggg aaggagctcg acgcaacagg ccgatgaggc
     4381 cttttggcgc tccaactacg aagaccacca gcatgatgcc aagaacgaga acgttgacct
     4441 cggatgaaat cgtcactgcc aggagttgct gggtggcacc cagcaggacc gcgccgagga
     4501 ctggcccgat ccagtgcgcc gtcccgccga tgagcgacat ggccagcacc gagacggagt
     4561 agttcaggtt gaaggcggat tccggattgg caaattgcag atacattgcc gcgggtgcgc
     4621 cgccggcaga gatcagcgca cccgatatca cgcaggccag aagcttcagc cgcagggtgg
     4681 gcacgcccgt acactcggcc gcgagttcgt cgtcgcggag cgcctgcagc ccgcggccaa
     4741 tccgggagtt ctggatggtc cgtgtggttg cgacggccac gaccaccaac agcgcctgca
     4801 cgaagaacag cattttgaca ta
//
LOCUS       DATLJT010001754         2881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1815377,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001754 DATLJT010000000
VERSION     DATLJT010001754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815377"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..992
                     /locus_tag="VM910_39785"
     CDS             225..992
                     /locus_tag="VM910_39785"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HVI68669.1"
                     /translation="MPRAIIEGDRVRITGVRNFDYRSRNDFTVRYEEREIRLSHLAAL
                     DFFVSYWSEGLVGHTFLSFIFDDAPPLSISIETRPEVGEGFSPIASMFKQFELIYVVG
                     DERDLVRVRTNYRNENVYLYRLNASAGDARRLLLAYLARINELADRPEWYHLLSNSCT
                     INIVRYANAAGRVGRFDIRHLLNGLVDGYLYHSGRLDTTLPFDELRRRSLINEAAQAA
                     DDAPDFSERIRASLPTISHPIRRTQTQNPAQSPPQGR"
     gene            complement(940..2393)
                     /locus_tag="VM910_39790"
                     /pseudo
     CDS             complement(940..2393)
                     /locus_tag="VM910_39790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            complement(2398..>2881)
                     /locus_tag="VM910_39795"
     CDS             complement(2398..>2881)
                     /locus_tag="VM910_39795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVI68670.1"
                     /translation="PTLFQAIEIDGESYWDGGYSGNPTITPLVRECRSQDTILVQINP
                     VERPGLPRSARDILNRLNEVSFNAVLLKELRMIALLRQVAQPDNSESAKWADMRIHRI
                     SSDVMVELGYSSKLNAEWEFLCMLRDEGRHAGDAFLSTHHDDLGRRSTFDLDELLKGV
                     "
BASE COUNT          566 a          900 c          915 g          500 t
ORIGIN      
        1 tcatcgcttg gggcaccttg gccatctact attcgaatct cccgtgggcc gcgctgcgct
       61 tgacgttagc ggcggccttt gcggcgttcg ccgtgtgggc ctgctgggtg tcgcctcgac
      121 gcggcatgtc cgcggttttt ctcggattat ttttcgtagt ggtcgtgtgg tggatctcga
      181 tccctccttc gcacgatcgc ccatggcggc cggaagtggc ggtgatgccg cgggcgatca
      241 tcgagggtga ccgcgtacgc atcaccggtg tgcgcaactt cgactaccgc agccggaacg
      301 actttaccgt gcgttatgag gagcgcgaaa tacggttgtc ccatttagca gcgcttgatt
      361 tcttcgtatc ctactggagc gaggggctgg tcggacacac attcttgagc ttcatttttg
      421 acgatgcccc gccgctgagt atctccattg agacgcggcc ggaggttggc gagggctttt
      481 cccccatcgc ctcgatgttc aagcaattcg agctgatcta tgtggtgggt gatgagcgtg
      541 atctcgtgcg cgtgcgcacc aactatcgca acgagaacgt atacctctat cgcctcaacg
      601 cctcggcggg cgacgcgcgg cggctgctgc tggcctatct ggcgcggatc aacgaactcg
      661 ctgatcggcc ggagtggtat catttgctga gcaacagctg cacgatcaac atcgtgcgct
      721 atgcaaacgc cgctggccgg gtcggccggt tcgatatccg ccatctccta aacggtttgg
      781 tcgacggtta cctctatcac tccggccgtt tggatacgac gctgccattc gatgaactgc
      841 ggcggcgatc cctgatcaac gaggctgcgc aggcggccga tgatgcgccg gatttttcgg
      901 agcgcattcg cgcatctctg ccgacgatct cccaccctat tagaaggacc caaacgcaga
      961 acccagcgca atcaccgcca caagggcgat aatcgcgcca actatggcca ccatgaccat
     1021 gtcgaaatag ctctcgcgat gcgtcaagcc gcacaatgcc agcagcgtca ccacggcgcc
     1081 gttgtgcggc aggctgtcga gggttccgga gccgatcacc gcaacacgat gcatgagcgc
     1141 gggatcgatg tctttcccgg ccgcaagctc catgtacgcg ttgcccagcg catcaagcgc
     1201 gatggtgagt ccgcctgagg ccgaaccggt gagcgccgcc agtacattgg tggcgacggc
     1261 caatgacacg agcggtcccc cctcgatcga cagcacccaa tcgcgaaccg ctgcgaaagc
     1321 gggcagcgcc gccaccacgg cgccgaagcc cacgagactt gcgatggtga cggccggcaa
     1381 taccgaggca ttggcgcccg cgtccatgct cgcgcgcagc gccggaaagt agcgataatt
     1441 gatggcgatg agcgtggcga tcgccgacgt cagcgcaacc accaccgccc aaacgccggc
     1501 gaccgccgag agcgaggtgg aaccccaacg ttcctccgcc aggaacgaaa agtcgaggcg
     1561 cggcagcagg agcagcgaca tcaagagatt aacactcact acgacgacca gaggcaggaa
     1621 tgcccgagcg atgggcggtt tgctgtcgcc gtgccggccg cgatggattt cggccggatc
     1681 gaactcgcgt gcggtggttg cgagctcgcg cacgaccggg tcgtccgccg cagcatcgac
     1741 cgccgtgccg tccgaagcgc cgtagccctc tccggcgcgg cgtgcggcac gctccgcccg
     1801 acccaaccac cacaggccga atcccagcat gatggcggat gcgatgattc ccagaccagg
     1861 cgcggcgaat ggcgtcgtgc cgaagaaggg cattgggatc gcattctgaa tcgccggcgt
     1921 tcccggcagg gctgacatcg tgaaggtgga ggtgcccagc gtgatcgccg ctggcatcaa
     1981 gcggcgggga atggacgccg ctcgaaacaa cgcctgcgcc atggggacga gcacaaagaa
     2041 tgcaacgaac aggctgacgc cgccataggt tacgagcgca cctgcgagta cgaccgccag
     2101 cacggcgcgg cgctcaccca gccgttcggt catgaagccg gcgatggcgg acacgcagcc
     2161 gctgtcgtcc atgagcttgc cgaacagggc gccaagtaga aacaacggga agaactgggc
     2221 caggaagacc gccgcactgc ccatgaaggt ttgggtccag ttggcgagca gcggctccag
     2281 cgcgaacgcg gcggcaagca gcgcggcggc gggagcagga ggagcacgct ccagccgcgc
     2341 agcgcaagcc atatgagcag cgccaatccg agcaagatgc cgatgagacc catggtctca
     2401 aacacccttg agcaactcgt cgaggtcgaa ggtggagcgt cggcctagat cgtcgtgatg
     2461 ggtgctcaga aatgcgtcac ctgcgtgccg cccctcgtcg cgcaacatgc acaggaattc
     2521 ccactcggcg ttgagcttcg aagaatagcc gagctccacc atcacgtcgc tggagatgcg
     2581 gtggatgcgc atgtccgccc atttggcgct ctcggaatta tccggctgtg caacttggcg
     2641 cagcaaggcg atcatgcgca gctctttcag aagcacggca ttgaacgaga cctcgttgag
     2701 ccggttgaga atgtcgcggg cagagcgcgg caacccaggc cgttcgaccg gattgatctg
     2761 aacgagaatc gtatcctggg agcggcattc ccgcaccagc ggcgtgatgg tgggattgcc
     2821 cgagtagccg ccgtcccaat agctttcgcc atcgatctcg atggcctgga acagcgtggg
     2881 g
//
LOCUS       DATLJT010001755         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2734234,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001755 DATLJT010000000
VERSION     DATLJT010001755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734234"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1241..1399)
                     /locus_tag="VM910_39800"
     CDS             complement(1241..1399)
                     /locus_tag="VM910_39800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68671.1"
                     /translation="MSQTGCAWPFGESCTVRIVYDDILNKHRMSYDAVFRHERTHSNG
                     WRHAQSRK"
BASE COUNT          331 a          518 c          497 g          313 t
ORIGIN      
        1 gacgttggtg cttcgggaaa accccgatgc ggacgttcct ggatgcaatg ccgatgacga
       61 aggagaaaat gatcgcggct taacgagaac ggacaccaaa atccgatcgc tcaaccaggc
      121 accgcctgtc agatcgagtt ccggctaata cacatatggc gtgctcaacc cacgtcgtgc
      181 cctgcttctt gatcgagctg gcgagtgacg aggtgggtcg cgcaagtagc tcaagcgcgg
      241 cgccgaatga ccggagcagt cttacggttc ccatccggtg ccgacgcgac ccgcatagac
      301 caacgcgcgg ccctcccggc gtgcaaggcg caactcctga agagccttcg ggcaaaaagc
      361 caattaccgc aaacctatct cgctgccagc atttcacctt gagccctatt gcgacttccc
      421 gctgtcccat gcgacccact cccgagttcg cacatcgtgt gcgcgagcaa cggttctggc
      481 gcggtcgctg gtccgattgg cctcaaagag caaatgtttg gcgtagagta acaaatcccg
      541 cacggaacgc gaagcgacta agaagcgtgc aatccggcaa gcgaatcgcc aatagcctcg
      601 gagatacaat gtcgaagcct aagacttgga tggaccacgc gcgctcgctt gaggcggact
      661 ggccgccccg ccagacgata actcttacgg acctagtcgc cgaagctcgc gccgccgagt
      721 gggattttac taattggtgt tacgcgagcg ggtacgacgc cagcgacccg gatgcccgcc
      781 gcatgttcga atgcttgctg gcttacgcga ggctcgaggg ccgcgccggc ttgcattgac
      841 cgccgctcgg ctgcacccgg ctgcatcacc atgctgaaaa ctctgatcat cgcgactgtg
      901 ctgctgccga cgctcgccgt cgcgcagcga tcccagcaac tcccgcaacc gagacaaccc
      961 ggccaatggt gtccggttgg gtggatggcg agcgggagct actgcgtacc gggctccgac
     1021 agggcgccgg ccgctattcc caagaagggt tggtgcccgg ccggctagcg cgagagcggc
     1081 agttattgca tccgctgagg gttcgcagct gcgagcatcc tagcatcagc ggcattttga
     1141 agtgattgcg cgggctgaaa acgccactcc atggatcggc tgcccccatt ccggtcccac
     1201 cgaagtcgaa cgggaacagg gtctcgtcca tggctcgctc ctattttctg ctttgggcgt
     1261 gccgccagcc gttggagtgc gtgcgctcgt gccggaagac ggcatcgtag ctcatgcgat
     1321 gcttattgag aatatcgtcg taaacgatcc gcacggtgca gctctcgccg aagggccacg
     1381 cgcagccggt ctgcgacacg ccccagcact ctactttgat gcctgcctcg tcggtgcgtc
     1441 gcgtcctcgg atctatcgct acaaatgaag gcggcttttt tgagcggtgg gatcggcaga
     1501 cggcgctgaa cccatcctca actcgaggga gcgcatcatg caagcttcgg cttcgaggcg
     1561 ggaaggtata ataagtagcg ccgatcaact tcgattgtcg tgtcgagccc cgcggagtgc
     1621 gcctgcccca tgcgccgaaa tccggcttcg gtcagttca
//
LOCUS       DATLJT010001756         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1815916,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001756 DATLJT010000000
VERSION     DATLJT010001756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815916"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VM910_39805"
     CDS             <1..99
                     /locus_tag="VM910_39805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVI68672.1"
                     /translation="SLRLLRAYSKIKDQTVQRQLVSLMESIAANEA"
     gene            complement(305..1288)
                     /locus_tag="VM910_39810"
     CDS             complement(305..1288)
                     /locus_tag="VM910_39810"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68673.1"
                     /translation="MLLTRRAAMRGLGRGAVTLIAAPASGQPAYPNRTIKMIVPYPPG
                     GTTDLLGRLVADQIKSGLSATVIVENKPGAATTLGAEQVARSEPDGYTLLMATSTTLA
                     INKTLYRKLAYDPAKDFAAVALVAAVPFALIINPSIPAKTLSEFIAYAKSNPGLAYGS
                     AGNGSPQHLGAEMLKSLAGIDIRHVPYRGSVPAMLDVIAGHIPFMVVDLQPALQQIRE
                     GKVRVLGVTTRARVAAAPEIPTLAEGGLAGYELVAWQGVVAPARTPRPIVDALATEIA
                     RLLADPATRDHFAAMALEPLPASTPDSFAAFIKTEIDRWAVIVKNSGAELE"
     gene            1478..2806
                     /locus_tag="VM910_39815"
     CDS             1478..2806
                     /locus_tag="VM910_39815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVI68674.1"
                     /translation="MQKIKIVAVATLLVAAPAFGESVSPTSPAPAKPATVPAGPARPT
                     ASKPAAPASTPESRSAAALALSHEPTYDEGTAERMKEAALSYTDIAVRGGWPTIANDA
                     KFAIGVAGANDDVLRKRLIMTGDLADDKTTGAFDDAVAEAVKRFQVRHGLAPTGSVTP
                     RTLAALNVPVQKRIKQLEASLERLESMNFTFGQRYVVVNIPATFAEAVEEDKVVRRYR
                     VIVGKTEKPAPTLTAEITGVVLNPTWTVPSSIAKSEISAHMRRDPSYLSRMHMEVLDA
                     HENPIDPQSVDWSGAHTPNFTVRQQNGAWNALGTVKIDMPNPYSVYMHDTNQRSLFND
                     DYRFDSHGCSRVDNVRDLAAWLLSEEVAKWNRAAIDAAIETGVHQEIPLPRKVPVAWI
                     YLTAWMTRDQTVQFRNDIYDQDEQLLEATAEEAAFLNQAANHPLTAHLAQ"
     gene            2918..>3374
                     /locus_tag="VM910_39820"
     CDS             2918..>3374
                     /locus_tag="VM910_39820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVI68675.1"
                     /translation="MLETPKHKHKSTKQKSYADGKILQDVTDGVGVVTFNNPGKRNAM
                     SLDMWEGLGHALTDLRDDEDVRVVILVGAGDKAFVSGADISQFEKTRHNAAASEEYSR
                     RSAAQRALLADYPKPTISCIRGFCLGGGMQVAMLTDIRIASQDSQFGIPA"
BASE COUNT          668 a         1080 c         1037 g          589 t
ORIGIN      
        1 agcctgaggc tgttgcgggc ctacagcaag atcaaggatc agacggtgca acgtcagctc
       61 gtatccttga tggaatcgat agccgccaac gaggcgtgat cgccgaacgg accggccagc
      121 gcggtctggt gtcccgaaca cgaagttcgc aggattttta cgaggggctg cgaacttcag
      181 gttcgaaagg acaccacacc agccgaactt acgttgcagc agttctgatg cgatcgatca
      241 gccgtggcga tcgagagtag acgtagccgg tggccgcatt cggccgcctg taggggctag
      301 ttgttcattc gagttcggcg cccgaatttt tgacaatgac cgcccagcgg tcgatctcgg
      361 tcttgatgaa tgcggcaaag ctgtcgggcg ttgatgccgg gagcggctcc agcgccatcg
      421 ccgcaaagtg atcgcgggtc gccggatcgg ccagcagcct ggcgatctct gtggcgagcg
      481 cgtcaacgat cggccgcggc gtgcgggcgg gcgcgaccac cccttgccag gcgacgagtt
      541 catagccggc gagaccgcct tccgcgagcg tcggtatttc gggcgcagcg gcaacgcgcg
      601 cgcgggtcgt cacgccgagg acacgcacct tgccttcgcg gatctgctgc agcgccggct
      661 gcaagtccac caccatgaag ggaatgtgtc cggcgatcac gtcgagcatc gccggcacgc
      721 tgccgcgata ggggacgtgg cggatatcga tgccggccag ggatttcagc atctcggcgc
      781 cgagatgctg cgggctgcca ttgcccgccg agccataggc caggcctggg ttcgacttgg
      841 cgtaggcaat gaattcggaa agcgtcttgg cagggatcga cgggttgatg atcagcgcga
      901 acggcacggc cgcaaccagg gccaccgccg caaagtcctt tgcgggatca taggcgagct
      961 tgcggtagag cgtcttgttg atggcaagcg tcgtcgaggt tgccatcaac agcgtgtaac
     1021 cgtcgggctc ggagcgcgcg acctgttcgg cgcccagcgt cgtcgccgct cccggcttgt
     1081 tctcgacgat cacggttgcg ctgagcccgg atttgatctg gtcggcaacc agacggccga
     1141 gaaggtccgt cgtgccgccg ggcggatagg gcacgatcat cttgatggtc cggttcggat
     1201 aggccggctg gcccgaggcc ggcgccgcaa tcagcgtgac ggctccgcgg ccaaggccgc
     1261 gcattgccgc ccggcgcgtc aacagcatgg ctcctccccg aaaccgttat ttttcgccag
     1321 cctcttcgca agctacatta ccggctaaag cgacatatca aaggccccca aagtccaatc
     1381 cgcaatttag gcgctgcaac agcttaatca gaacgcaatt ctctccgaat agaagaccgt
     1441 gcctcttcac ccttcgcgcg gctcctgggg atcatccatg caaaaaatca agatcgttgc
     1501 cgttgcaacc ctgcttgtgg ctgcgcccgc attcggcgag agcgtttccc ccacttcgcc
     1561 tgctccagcc aagccggcaa ccgtgcctgc tggccctgct cgcccgaccg catccaagcc
     1621 ggccgcgccc gcctccaccc cggaatcgcg ctcggccgca gcactcgcgc tgtcgcacga
     1681 gccgacctat gacgaaggca ccgcggagcg aatgaaggaa gcggcgctca gctatacgga
     1741 tatcgccgtg cgcggcggct ggccgaccat cgccaacgat gcgaaattcg cgatcggcgt
     1801 ggcgggcgcg aacgacgatg tcctgcgcaa gcgtctgata atgaccggcg acctcgccga
     1861 cgacaagacg acgggcgcgt tcgacgatgc ggtcgccgag gcggtcaagc gtttccaagt
     1921 ccgacacgga ttggcgccga ccggcagcgt cacgccgcgc acgcttgcag ccctcaacgt
     1981 gcccgtgcag aagcgcatca agcagctcga ggcctcgctc gaacgcctcg agagcatgaa
     2041 tttcacgttt ggtcagcgtt acgtcgtcgt caatattccc gcaacctttg cggaggccgt
     2101 cgaggaggac aaggtcgtcc ggcgctatcg ggtaatcgtc ggcaagaccg aaaaacccgc
     2161 gccgaccctg actgccgaga tcaccggcgt cgttctcaat ccgacctgga ccgtgccgtc
     2221 gtcgatcgcg aaatccgaga tttccgcgca tatgcggaga gacccgagct atctctcgcg
     2281 catgcatatg gaagttcttg atgcgcacga gaatccgatc gatccgcagt ctgtggactg
     2341 gtccggcgcg catacgccga acttcacggt gcgtcagcag aacggcgcct ggaacgccct
     2401 cggtacggtc aaaatcgaca tgccgaatcc ctattcggtc tacatgcacg acaccaatca
     2461 gcggagcctg ttcaacgacg actaccgctt cgattctcac ggctgctcgc gcgtcgacaa
     2521 tgtccgcgac ctcgccgcct ggttactctc cgaagaggtg gcgaaatgga atcgggcggc
     2581 aatcgatgcc gcaatcgaaa ccggagtgca tcaggaaatt ccgctgccga ggaaggtgcc
     2641 ggtcgcctgg atctatctca cggcatggat gaccagggac cagaccgtgc agttccgcaa
     2701 cgacatctac gaccaggacg agcagcttct ggaagcgacc gccgaggaag ccgccttcct
     2761 caatcaggct gccaatcacc ctttgactgc gcatctggcg caatgaacgc gcgttgcgat
     2821 gcccgacgcg cagagctgga cgcgcgcagg agcattttcg cgcgccttgc catcggctag
     2881 agtgaggtca gtcgttctca ccaaaagcgc ctttcaaatg ctggaaaccc caaaacacaa
     2941 gcacaagtcg accaagcaaa aatcctacgc cgacggaaag atcctgcagg acgtcaccga
     3001 cggcgtcggc gtcgtcacct tcaacaatcc cggcaagcgc aacgccatgt cgctcgacat
     3061 gtgggagggt ctcggacatg cgctgaccga tctgcgcgac gatgaggacg tgcgggtggt
     3121 gatcctcgtc ggcgccggag acaaggcatt cgtctcgggc gctgacatca gccagttcga
     3181 aaagacccgc cacaatgcgg cggcttcaga ggaatattcc aggcgcagcg ccgcgcagcg
     3241 ggcattgctg gccgattatc cgaagccgac gatttcctgt atccggggtt tctgtctcgg
     3301 cggcggcatg caggtcgcca tgctcaccga catccgcatc gcctcccagg acagccagtt
     3361 cggcattccc gcgg
//
LOCUS       DATLJT010001757         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2961554,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001757 DATLJT010000000
VERSION     DATLJT010001757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2961554"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            628..1431
                     /locus_tag="VM910_39825"
     CDS             628..1431
                     /locus_tag="VM910_39825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HVI68676.1"
                     /translation="MAVHSMTRRTALGAASMGVSSLIATQAMAQENPRKTFVLVHGAY
                     HGGWCWRRVADLLEVRGHKVYAPSLTGNGDRSHLLSKDINLDTQIADIANLATWEDLN
                     DLCLVAHSFGGWPASGALEHIHDRVGSIVWLDAFKPEDGQKGVDYISEFSRKAMEEAV
                     ARGEPGRKPPPAKTFSLSEKDYAWIDSKLTQQPNGLVMQPIKLTGKRDAIAKKTYIRA
                     PKYPQTAFDKALAECKAGGGWQTFINDNSGHDVMIDQPAWLANLLLKVS"
     gene            1526..2290
                     /locus_tag="VM910_39830"
     CDS             1526..2290
                     /locus_tag="VM910_39830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68677.1"
                     /translation="MAGQLQGKIAIVTGASNGIGRGIAELFAVEGAKTVLVARRAELL
                     DEVAAGIKAKGGEALPLPADLTKEDAIIALFKKTVETFGRLDVLVNNAGIATHRNTED
                     ITLDYWNEALAINITAPFLCAREAVRIMKAQQPQGGRIVNIGSVSAKTPRPDSLPYTV
                     TKFGLQGMTHQLTMDGRKHNIVSSIIHPGATLSSFSTRRGRTKAGYGEKPGDDYIMAA
                     EDVAKVAVLMAALPDEVNLYEATILPNTMCSFIGRG"
     gene            complement(2415..>2595)
                     /locus_tag="VM910_39835"
     CDS             complement(2415..>2595)
                     /locus_tag="VM910_39835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68678.1"
                     /translation="ALLCARDEWESHGSAAECQNKFASLHCLTGRSGSRIVTGPTSKP
                     EEGWSYGWSMSALGQ"
BASE COUNT          556 a          775 c          782 g          482 t
ORIGIN      
        1 gcaggctttc caaggccttc agccgaagcg cgggctctga aggcaggtga ctatggagct
       61 cttgatggat gtgcgaaatg atatctgagt catgcctggt catggtggtg atccgccagt
      121 taaggtcttt caccagcgtg cgatactact acgagagtca cagtagtccc agggcgggat
      181 tgactcgccg accggggcct ccatcggaaa tagtggaccc tgctgaattt aatgggcatc
      241 caactgccga tcatggacgg ctacgaggtt acccggcaaa tcaaccgcgt cgcgatcgat
      301 ttcaatcatc gccatcactt cccacacgct ggatggggaa gaacagacca cgcgagcagc
      361 aggatgtgac agctatatgc cggagcctta cagcccgcga caactgctgg cgaaaaaatt
      421 cagcagtgca tgccctaata gctgcgatcg attgctgcac cgcttaatgt cgcttttggc
      481 acaaagcgga catcacacct cgactgatcc atgtccgctt tcgggggtaa agcggacatt
      541 gagagcggta gttttttgct atcgtattgc gctcactgca ataagacggc ttgtcttaac
      601 gacggcgaga agagacagga ggaagcgatg gcggtccatt caatgacgcg ccgcacggcg
      661 ctgggcgcgg cgagcatggg tgtgtcgtca ctcatagcga cgcaggcgat ggcacaagag
      721 aacccgcgca agaccttcgt gctcgtgcac ggcgcctatc acggcggttg gtgctggcgc
      781 cgcgtcgctg acctgctcga agtgcgcggt cacaaggttt atgcgccgtc gctcaccggc
      841 aatggcgacc gctcgcatct tctcagcaaa gacatcaatc tggacactca gatcgccgac
      901 atcgccaatc tcgcgacgtg ggaagacctc aacgacctct gcttggtcgc gcattcattc
      961 ggcggctggc cggcctctgg cgcgctcgaa catattcacg accgcgtcgg ttcgatcgtc
     1021 tggctcgacg cgttcaagcc ggaggacggc cagaagggcg ttgactacat ttcagagttc
     1081 agccgcaagg ccatggagga ggcggtggcg cgcggcgagc ctggccgcaa gccgccgccg
     1141 gctaagacct tctcgctcag cgagaaggat tatgcctgga tcgactccaa gctgacgcag
     1201 cagccgaatg gtctagtgat gcagccgata aagctcaccg gcaagcgcga cgcgatcgct
     1261 aagaagacct acatccgcgc gccgaaatat ccgcagactg ccttcgacaa ggcgctggcc
     1321 gaatgcaaag ccggcggcgg ttggcagacc ttcatcaacg acaattccgg ccatgacgtg
     1381 atgatcgatc agccggcctg gctcgcgaat ttgttgctga aagtgtcgta ggtcgcagcg
     1441 gaccgtgctg gcgcaagtct aacggttatg cccataacgg gcctttcgtg agtagtggca
     1501 tttgcgcgat ttacgggagt gcatgatggc cggacaactc caaggcaaga tcgcgatcgt
     1561 caccggcgcc agcaacggca tcggccgcgg catcgccgag ctgttcgccg tcgaaggcgc
     1621 gaagaccgtg ctggtggcac gccgcgccga gctgctggat gaggtcgcgg caggcatcaa
     1681 agcaaaaggc ggcgaagcgc tgccgctgcc ggccgacctc accaaagaag acgcgatcat
     1741 cgcgctgttc aaaaagacgg tcgagacctt cgggcgcctt gacgtgctcg tgaacaacgc
     1801 tggcatcgcc acccacagga acaccgagga catcacgctc gactactgga acgaggcgct
     1861 cgccatcaac atcaccgcgc cgtttctctg cgcgcgtgag gcggtccgca tcatgaaggc
     1921 gcagcagccc cagggcggac gcatcgtcaa catcggcagc gtttcggcca agaccccgcg
     1981 gccggattcg ctgccctaca cggtgaccaa gttcggcctg cagggcatga cgcaccaact
     2041 cacgatggat gggcgcaagc acaatatcgt ctcgtccatc atccatcccg gcgccacgct
     2101 gtcgtcgttc tcgacacggc gcggacggac caaggccggc tacggcgaaa agcctggcga
     2161 cgattacatt atggccgccg aggatgtggc caaggtcgcg gtgctgatgg ctgccctgcc
     2221 ggacgaggtg aatctctacg aggcgacgat cctgccaaac accatgtgtt cgtttatcgg
     2281 gcgagggtaa gaccacgccg gtccagcagg cccataaccc ctgtcgggcc gcgctgatga
     2341 gttaatcgaa tagctatcga atgtctgctt ttgggggtaa agcggacatc ggttcaaaac
     2401 ctcatcgatg tccgttattg acccaaagcg gacattgacc agccatagct ccacccctct
     2461 tccggtttac tggttgggcc agttacgatg cgtgatccag agcgtccggt caggcaatga
     2521 agcgacgcga atttgttttg acactcggca gcgctgccgt ggctttccca ttcgtcgcgc
     2581 gcgcacaaca gcgcg
//
LOCUS       DATLJT010001758         1723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2502087,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001758 DATLJT010000000
VERSION     DATLJT010001758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502087"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..781)
                     /locus_tag="VM910_39840"
     CDS             complement(89..781)
                     /locus_tag="VM910_39840"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HVI68679.1"
                     /translation="MKGGDIVAAVLGCIAISLTAIAFGSGPSYAAGVATEASDVEVST
                     ALIREIQFMLLRLGMDPGPIDGIPGPQTLSAVRKFEVKSGLPASDPITDRKISSVFLA
                     RLREEASRAILGGEGKPEASPAVATPSPVAVAPAQPPAPPPDRFAACTYNPGDFRIGT
                     TQYTPDNFLQVGFDGSTARAVSSLKDRLNEARQLAENLGGSALAEVQRQARVLDYFNC
                     RLKIEQASDGKK"
     gene            complement(797..>1723)
                     /locus_tag="VM910_39845"
     CDS             complement(797..>1723)
                     /locus_tag="VM910_39845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVI68680.1"
                     /translation="EVKVADFGIARIDASEFTIVGDLLGTPAYMAPEQFAGAPVDRRT
                     DLFAAGVILFEMLAGVKPFRGKSITEIISFMESRGPEDIRALNPAVPDSLKRVITKAL
                     SFDPAGRFNDAAEFSKAILEAFPVRVAQTPVPEPLVSATAVENADIGSTASVDATWRP
                     DLLREIERDLATFIGPVAAIAVRRAIRQTSDVAAVYDLLGRQVANPRDRAEFLARGQR
                     RAISQPDSRPRSTPEAQVEPMPDRATTSLGLTNIALVESNLARYIGPIAKILIKRELE
                     KHGTLDQFYRALAAHIPDERERENFLRAQHRN"
BASE COUNT          324 a          538 c          527 g          334 t
ORIGIN      
        1 acgcaccgct ccgacatggg ttcgcgcggc cgagcggtaa cgcggcttgt tgtcgatcgc
       61 agtcacggtt tcctgggcgc agcgaggcct atttctttcc gtccgacgcc tgttcgattt
      121 tcagccgaca attgaagtag tcgagaacgc gcgcctgccg ctgaacttcg gcgagcgcgg
      181 agccgccgag gttttcggca agctggcgcg cttcgttgag acggtccttc aagctcgaaa
      241 ccgcgcgcgc cgtcgaaccg tcaaatccca cttgcagaaa gttgtccggc gtatattggg
      301 tcgtcccgat ccggaagtcg ccgggattgt aggtgcacgc agcaaatcga tctggcggcg
      361 gagcgggggg ctgagctggt gccaccgcaa ccggtgaagg cgttgcaacc gctggcgaag
      421 cctccggctt cccttcgccg ccgagaatgg ccctggatgc ttcctcgcgt aaccgcgcca
      481 ggaagacgct tgaaattttc ctgtcagtga tcggatccga ggccggcaga ccggatttta
      541 cctcaaattt gcggactgcg ctcaaagtct gcggaccggg aataccgtcg atgggtccgg
      601 ggtccattcc gagccgcagc agcatgaatt ggatctcgcg gatcagcgct gtgctcactt
      661 cgacatccga agcctctgtc gcgacaccag cagcataaga cgggcccgag ccgaacgcta
      721 ttgccgtcaa agagatcgcg atgcagccga gcaccgccgc gacaatatcg ccccccttca
      781 tctgcgcctt tccttctcag ttgcggtgct gggccctcag aaaattctct cgctcgcgtt
      841 cgtccggaat atgcgccgcc agcgcgcggt agaattgatc cagcgtcccg tgtttttcga
      901 gctccctctt gatcaggatt ttcgcgatcg ggccgatgta gcgggcgagg ttcgactcga
      961 ctaaagcaat gttggtgaga ccgagtgatg tggtcgctcg atctggcatt ggttcaacct
     1021 gtgcctctgg cgtcgagcgc ggccggctgt ccggttggga gatggcccgc cgttgccccc
     1081 gcgccaggaa ttcggcgcgg tcacgcgggt tcgccacttg ccgtcccagc aaatcgtaca
     1141 ccgccgcaac gtcactggtc tgccggattg cccgccgcac cgcaatcgcc gccaccggac
     1201 cgatgaatgt cgccagatcg cgctcgatct cgcgcaacag atcgggacgc caggtcgcgt
     1261 ccactgacgc ggtcgacccg atatcggcgt tctcgaccgc cgtggccgag accagcggtt
     1321 cgggaacagg cgtctgtgca acgcgaacag gaaaagcttc gagtatcgcc tttgagaact
     1381 cggcggcatc gttgaagcgc ccggccggat caaaggacaa ggccttggta atgacccgct
     1441 tcagcgaatc gggaacagcc ggattcaagg cgcggatatc ttcaggtccg cggctctcca
     1501 tgaacgaaat gatctcggtg atgcttttgc cacggaaagg cttcactccg gccaacatct
     1561 caaacaggat gacaccggcg gcaaagagat cggtgcgcct gtcgaccggc gctccggcaa
     1621 actgctccgg ggccatataa gccggcgttc ccaataaatc gccgacgatg gtgaactcgg
     1681 aggcgtcgat gcgggcgatg ccgaagtcgg cgaccttaac ctc
//
LOCUS       DATLJT010001759         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1357778,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001759 DATLJT010000000
VERSION     DATLJT010001759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357778"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VM910_39850"
     CDS             complement(<1..458)
                     /locus_tag="VM910_39850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68681.1"
                     /translation="MAKGFASTTDLQEKKITFSEIGPDLYAFTAEGDPNSAVIVGEDG
                     CLVFDAQATPAMANKVIEQVKAVTDKPIKYVVLSHYHAVRVLGASAYHAEAIIASQET
                     RRLVAERGKQDWDSEYGRFPRLFQDAQSIPGLTWPTLTFEGDMSIYLGKR"
     gene            complement(485..712)
                     /locus_tag="VM910_39855"
     CDS             complement(485..712)
                     /locus_tag="VM910_39855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HVI68682.1"
                     /translation="MPLSTSSNFARPDDAFRAIVEAHRGLTDEQSADLDAALVLILAN
                     HIGDIAVLSEAIALASRRMITADQQQQQQQQ"
     gene            complement(713..2362)
                     /locus_tag="VM910_39860"
     CDS             complement(713..2362)
                     /locus_tag="VM910_39860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVI68683.1"
                     /translation="MAQAETQARNQTKTQFGYRRHADQDRTGADIAEHRVIVVGAGPV
                     GLSLAIDLAQRGQDVLLLDDADRIGEGSRAICFSKRSLEFWDRLGVGDRMVDKGVVWS
                     VGRIFHGDRQLYQFNLLPEQGHKRPAFINLQQFYAEAYLVDRVEQLPSISLRWCNKVV
                     ALEQRNDGVALTIDTPDGPYQVRAAYVIACDGARSSLRGMVGAEFSGQVFEDQFLIAD
                     VKMTAEFPTERWFWFDPPFHAGRSALLHKQPDDIWRIDLQLNRYADPAVEKQPEHVRP
                     RIARMLGHDKFEFEWISLYKFQCRRMDRFIHGRVIFAGDAAHQVSPFGARGANSGLED
                     AENLAWKLDRVLRGQSPEILLESYHVERSAAADENIRESTRSTDFMAPNSHQEARLRK
                     AVLSLAKETEFGKRMVNGGRLSVPSIYESPLSTPDGEDWRGGPQPGTSMPDAPIAAQS
                     GQPMFLTDAFIRQGTRFTLLEFGNGASVAAPEGLGAIRICGQDGFVDRDGLATARYDA
                     EPGTAYLLRPDGYVAARFRHPTRDALEAALARAVGLNGRPS"
BASE COUNT          438 a          765 c          769 g          454 t
ORIGIN      
        1 tcgcgcttgc cgagatagat cgacatgtcg ccttcgaaag tcagcgtcgg ccaggtcagc
       61 ccggggatgc tttgcgcatc ctggaacagg cgcgggaagc ggccatattc ggaatcccaa
      121 tcctgcttgc cgcgctcggc aaccagacgg cgcgtttcct gcgaagcgat gattgcttcc
      181 gcgtgatagg cggatgcgcc gagtacgcgc accgcgtggt agtgagacag caccacatat
      241 ttgatcggct tgtcggtgac ggcctttacc tgctcgatca ccttgttcgc catcgcgggc
      301 gttgcctgcg catcgaacac gaggcagcca tcctcgccaa cgatcactgc cgaattgggg
      361 tcgccttcgg cggtgaaggc atagagatcg gggccgatct cggagaaagt gatttttttc
      421 tcctgaaggt ccgtggtcga cgcgaagccc tttgccatcg gctcagtcct ttgaatcccg
      481 gcgattattg ttgctgctgc tgctgttgct ggtcggcagt aatcatgcgt cggcttgcga
      541 gcgcaatcgc ctcgctcaac acggcaatat cgccgatatg gttagcgagg atcagaacca
      601 gcgcggcatc gagatcggcg ctctgttcat cggtcaggcc gcggtgcgct tcgacaatgg
      661 cgcgaaacgc gtcgtccggc ctcgcaaaat tcgaactcgt ggacagcggc atttagctag
      721 gcctcccgtt caggccgacc gcgcgggcga gcgcggcctc aagggcatcg cgcgtcgggt
      781 gacggaaacg cgccgcgaca tagccgtcgg gccgcagcag ataggcggtt ccgggctccg
      841 cgtcgtagcg cgcggtcgcg agcccgtcgc gatcgacgaa gccgtcctgg ccgcagatcc
      901 ggatcgcgcc gaggccttcg ggcgcagcga ccgatgcacc attgccaaac tcgagtaatg
      961 tgaagcgcgt tccctgcctg atgaaagcat cggtcaggaa catcggctgc cccgattgcg
     1021 ccgcgatcgg cgcatccggc atagacgtac cgggctgcgg ccctccgcgc cagtcctcgc
     1081 cgtccggcgt cgacaatggg gactcgtaaa tcgatggcac cgagaggcgc ccgccattca
     1141 ccatgcgctt gccgaactcg gtttccttcg cgagcgagag cacggctttg cgcagccgcg
     1201 cctcctggtg cgagttcggc gccatgaaat cggtggagcg ggtcgattcg cggatatttt
     1261 cgtcggccgc agcgctccgc tcgacgtgat agctttcgag cagtatctcc ggcgattgcc
     1321 cgcgcagcac gcgatcgagc ttccaggcga gattctcggc atcctccagc cctgaattgg
     1381 cgccgcgcgc gccgaacggg gagacctggt gggcggcatc gccggcaaag atcacgcggc
     1441 cgtggatgaa acggtccatg cgccggcact ggaatttgta caacgagatc cattcgaact
     1501 cgaacttgtc gtggccgagc attcgcgcga tgcgcggccg tacatgctcc ggctgcttct
     1561 cgacggccgg atcggcataa cgattgagct gaaggtcgat ccgccagata tcgtcgggct
     1621 gcttgtgcag aagcgccgag cgcccggcat ggaacggcgg gtcgaaccag aaccagcgct
     1681 cggtggggaa ttccgccgtc atcttcacat cggcgatcag gaattggtcc tcgaacacct
     1741 gtcctgaaaa ctccgcaccc accatccccc gcaacgagga gcgcgcgcca tcgcaggcaa
     1801 tgacgtaagc ggcacgcacc tgataggggc catccggcgt atcgatggtg agcgcgacgc
     1861 cgtcgttgcg ctgttccaac gctactacct tgttgcacca gcgcaaggag atggagggaa
     1921 gctgctcgac acggtcgacg agataggcct cggcgtaaaa ctgctggaga ttgatgaagg
     1981 ccggccgctt gtggccttgt tcgggcagca ggttgaactg atagagctgt cggtcgccat
     2041 gaaagatcct gccgacactc cacaccacgc ccttgtcgac catgcggtcg ccgacgccga
     2101 ggcggtccca gaactcgagc gaccgcttgg agaagcagat cgcgcgcgag ccttcgccga
     2161 tgcggtcggc gtcgtcgagc agcaggacgt cctggccgcg ctgcgcaagg tcgatcgcca
     2221 gcgacagccc cacgggaccc gcgccaacca cgatgacccg atgttcggcg atgtcggcac
     2281 cggtccggtc ctggtccgcg tggcggcggt agccgaactg ggtctttgtc tgattcctcg
     2341 cctgtgtttc cgcctgcgcc atctcttcaa accttcgcac tagccagacg gcggcgggtt
     2401 tgttaaatta gtctcagttg caacta
//
LOCUS       DATLJT010001760         4546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4343487,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001760 DATLJT010000000
VERSION     DATLJT010001760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4343487"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VM910_39865"
     CDS             <1..232
                     /locus_tag="VM910_39865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HVI68684.1"
                     /translation="NGLDGRKSGSVAGYSYSDANKGSGITWNDASFLEYIKDPKAKIP
                     NTKMTFAGIKNEDEAKNLWAYLKQYDVDGKTK"
     gene            complement(268..1272)
                     /locus_tag="VM910_39870"
     CDS             complement(268..1272)
                     /locus_tag="VM910_39870"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HVI68685.1"
                     /translation="MHLDKPTRRRAITIFAAAAAGAITGGPHRLAKADFAWRGVAMGA
                     DATILFNGIEPEAAKAATALVEAEIDRLEGALSLYRPDSELCRLNRDRMLLRPSGDLR
                     RALGIALDVSAMCDGLFDPTVQTLWETYVNWFTASPGAGLPPDSIIALAHAAVDWRKI
                     SVKADAIRSGPDQRLTLNGLGQGYLTDRVAELLATQGLKHVLVDLGEQRALSPRQSGA
                     PWLVTRGGAHTIRLMRGALATSEGSGCVLGAGGAVHHLFDPRTGRSAAHWKRITVHHR
                     SAAIADALSTALYIASAGDIKAMLPRVPDVIVWARDHDGREWRWSSSPVDGVALEEPG
                     "
     gene            complement(1282..3396)
                     /locus_tag="VM910_39875"
     CDS             complement(1282..3396)
                     /locus_tag="VM910_39875"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVI68686.1"
                     /translation="MQGTIGQTGLSALFGLLRGLAIVILMAGAAQAADLTIASQFFSS
                     TGPVTVEPPQGSPPSAIVRASDGHILGYAFSTLDVSGSVGYAGRPLDIVAAVTPEGIV
                     AGARIVAHEEPILVIGIPRDALAAYVAGFGGFDVRAGAGLKPADDLARGPHAVAGATI
                     TSTVIRDAIVRSSRTVLRSRDNAPDGTARLDRETLRRSSWQSLVAEGTVQHRLVLRAE
                     ASKLLGTQDSEPDKPFIDLWLALATPPPIGESLLGQRIYESELAKIGPDDDLVLIGAS
                     GLYSFKGTEWRQSGSFERFEIIQGSRTLRLKAADHTPIEALHAAGAPELREIAVFRIP
                     RSSGFDSTKPFRLDLDLGTPAQASGPAVVTLDYRIPDRYLIGPAATPVQPSAGRTAAS
                     AAAQPPVWQEIWWARRYEIAVLGAMLTVLAGILVFQDTVTAHGAFYYRLRTSYMLLTL
                     LFLGFFANAQLSVVNVLTFIHALLSGFRWELFLLDPMVFTLWSFVAVSMLFWGRGVFC
                     GWLCPFGTLQELTNHLAQRLGIKQIEIPFGLHERLWMIKYVVFVGILALSLRSILLAF
                     QLAEVEPFKTAITMKFAREWPFVLYAGLLVFAGLFVERFYCRYLCPLGAALAIPARMR
                     MFEWLKRYRECGSECQVCARRCTVQAIHPLGQINPNECIYCLKCQANYFDHEICLHLK
                     KRAQRRQPQTTASPANSNAPRT"
     gene            3527..>4546
                     /locus_tag="VM910_39880"
     CDS             3527..>4546
                     /locus_tag="VM910_39880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HVI68687.1"
                     /translation="MRSRAVVFAMLTGAIAIASGFYLAPTGTAYAQAQPAAPATPKPA
                     DIEQHKTTPGGQYQPSLDVLGGKPTEQPGTKPGDPNLTKAEFDQANRIYFERCAGCHG
                     VLRKGATGKPLTPDLTKKLGYEYLRDFITYGSPGGMPNWGSSGQLSENEVALMARYLL
                     NEPAQPPEFGLKEMKESWKVLVPVAQRPTKKMNKLDIDNLFSVTLRDAGEVALIDGNT
                     KKIEYILQSGYAVHISRMSASGRYLYIIGRDAKLNVIDLWMDPPQTVAELKIGTEARS
                     VETSKFKGYEDRYAIAGAYWPPQYVLMDGATLEPLKVVSTRGMTYDTQEYHPEPRVAS
                     IVASHY"
BASE COUNT          930 a         1394 c         1461 g          761 t
ORIGIN      
        1 gaacggcctc gatggccgaa agtcgggatc ggtggcgggg tacagctatt ccgacgccaa
       61 caaggggtcc ggcatcacct ggaacgacgc gagcttcctg gagtacatca aggatcccaa
      121 ggccaagatt ccgaacacca agatgacctt cgcaggcatc aagaacgaag acgaagcgaa
      181 gaacctgtgg gcctatctca agcaatatga tgtcgacggc aagaccaagt aactacgagc
      241 tttgccgatg cttcggtgcg atgcgcgtca gcccggttcc tcgagcgcga cgccatccac
      301 cggagatgaa ctccagcgcc attcacgacc gtcgtgatcg cgggcccaca cgatgacatc
      361 cgggacgcgc ggcagcatcg ccttgatgtc gccggccgag gcgatgtaga gcgcggtcga
      421 cagcgcgtcg gcaattgccg ccgaccgatg atgaactgtg attctcttcc aatgtgcggc
      481 gctgcggccg gtgcgtggat cgaacaggtg gtgcaccgcg ccgcccgcgc cgagcacgca
      541 gcctgagccc tccgaggttg caagcgcgcc gcgcatgagg cggatcgtat gggcgccccc
      601 gcgggtcacc agccaggggg cgccgctttg gcgcggggac agtgcccgct gctcgccgag
      661 atcgaccaga acgtgcttca agccctgcgt ggcgagcagt tccgcgacgc ggtcggtcag
      721 gtaaccttgg ccgagaccat taagcgtcag gcgttggtcc ggtccgctgc ggatcgcgtc
      781 cgccttgacg ctgatcttgc gccaatcgac cgccgcgtgc gccagtgcga tgatcgaatc
      841 cggtggcagg cctgcgccgg gagatgcggt aaaccagttg acataggttt cccagagcgt
      901 ctgcacggtc ggatcgaaca agccatcgca cattgccgaa acgtcgagtg cgatgccgag
      961 tgcgcgccgc agatcgccgc tcggccgaag cagcatgcga tcgcgattga gccggcacag
     1021 ctcggaatcc ggccgataca ggctcagcgc accctcgagg cggtcgattt cagcttcaac
     1081 gagcgcggtc gcggcctttg ctgcctccgg ttcgatgccg ttgaagagga ttgtcgcgtc
     1141 cgcgcccatc gcgaccccgc gccatgcaaa gtcggccttg gcgaggcggt gaggaccgcc
     1201 cgtgattgcg ccggcggctg ctgcagcaaa gatggtgatg gcacgccggc gggtcggctt
     1261 gtcgagatgc atcggcgtct cctatgtccg cggcgcgttg ctgttcgcgg gcgaggccgt
     1321 cgtctggggc tgccgccgtt gcgcgcgttt tttcagatgc aggcagatct cgtggtcgaa
     1381 atagttggct tggcatttga ggcagtagat gcactcgttc ggattgatct gccccagcgg
     1441 atgaatggcc tgaacagtgc agcggcgcgc gcagacctgg cattccgagc cgcattcacg
     1501 ataccgcttg agccattcga acatccgcat gcgtgcggga atggccagag ccgcaccgag
     1561 cgggcaaagg taacggcaat agaaccgctc gacgaagagc ccggcgaata ccaggagccc
     1621 cgcatagaga acgaacggcc actcgcgcgc gaacttcatg gtgatggcgg tcttgaatgg
     1681 ttcgacctct gccagctgaa acgccagcag gatcgagcgc agcgacagcg caaggatgcc
     1741 gacgaaaacc acgtatttga tcatccagag ccgctcatgc aacccgaacg gaatttcgat
     1801 ctgcttgatg ccgaggcgct gcgccaggtg attggtcagt tcctgcagtg tgccgaacgg
     1861 gcaaagccag ccgcagaaca cgccccgtcc ccagaacagc atgctgaccg caacgaagct
     1921 ccagagcgtg aacaccatcg ggtcaagcag gaacagctcc caccgaaagc cggacaggag
     1981 tgcgtgaatg aaggtcagca cgttgacgac cgacaattgc gcgttggcga agaacccaag
     2041 gaagagcagc gtgagcagca tgtaggaggt gcgcagccgg taatagaatg caccatgtgc
     2101 ggtgaccgtg tcctgaaaca ccagtatgcc ggcaagaacc gtgagcatcg cgccgagcac
     2161 tgcgatctcg tagcgccgtg cccaccagat ctcctgccat accggcggct gtgcagcagc
     2221 agatgccgcg gttcggcccg ccgagggctg gaccggggtt gcggcaggac cgatcaggta
     2281 tcggtcaggt atgcggtaat ccagcgtcac gacggccggc cctgaggcct gtgccggggt
     2341 tccgagatca agatcgagcc ggaatggttt ggtcgagtcg aacccgctcg atcgcgggat
     2401 acggaagacc gcgatctcgc ggagttccgg cgcgcccgct gcgtgcaagg cttcgatcgg
     2461 cgtgtggtcg gcggccttca aacggagggt gcgactgccc tggatgatct cgaatcgttc
     2521 gaacgaaccg gactgccgcc attcggttcc cttgaacgaa tagagacccg atgcgccgat
     2581 cagcacgagg tcgtcatcgg gaccaatctt tgccagctcg ctttcgtaga tgcgttgccc
     2641 gagcaggctc tcgccgatcg gcggcggtgt ggcgagcgca agccagagat cgatgaatgg
     2701 cttgtctggc tcgctatcct gcgtgccgag caactttgac gcctcggcgc gcaacaccag
     2761 ccgatgttga accgttccct cggccacgag cgattgccac gacgaccgac gcagggtttc
     2821 gcggtcgagc cgtgccgtgc cgtcaggtgc gttgtctcgg ctgcgcagca ccgtacgcga
     2881 tgagcgcacg atcgcatcgc gaatgacggt gctggtgatt gttgcgcctg cgaccgcgtg
     2941 cgggccgcgg gcaaggtcgt cagccggttt gaggcccgcg ccggcacgga cgtcgaaacc
     3001 tccgaaacct gccacatagg cggcaagcgc gtcacgtgga atacctatca caaggatcgg
     3061 ctcttcgtgt gcgacgatcc tggcgccggc aacgatgcct tccggcgtga cggccgcaac
     3121 aatgtcgagg gggcgcccgg catatccgac tgagccggac acatcgagcg tggagaacgc
     3181 gtaaccaaga atatgaccgt ccgatgcccg tacgattgcg cttggaggac ttccctgcgg
     3241 cggctccacg gtcaccggac cagtggagga aaagaattgg gaagcaatcg tgaggtcggc
     3301 tgcttgcgcg gcgccagcca tcaggatgac gattgcgaga ccgcgcagga gtccgaatag
     3361 cgcagaaagc ccagtctggc cgatcgtgcc ttgcatggcc ggatcaggca gtgtcccccg
     3421 actgctgcat tgagatagat caagtcgcta cttaaccgcg gtcaaggcag caaagctccc
     3481 ttcgaggcat aagccaactt gcggagtctc atgctggagg gatgcgatgc ggagtcgcgc
     3541 ggttgtgttt gcaatgctca cgggcgctat cgccatcgcg agtgggttct atctggcgcc
     3601 gacgggcaca gcatacgcgc aagcgcagcc ggcggcgccg gccacaccaa agcccgccga
     3661 catcgaacag cacaagacga cgccgggtgg ccaataccag ccgagcctcg acgtgctcgg
     3721 cggaaagccc accgagcagc ccggcaccaa accgggcgac cccaatctca ccaaggctga
     3781 attcgatcag gcgaaccgga tctacttcga acgttgcgca ggctgtcacg gcgtgctgcg
     3841 caagggtgca accggcaagc cgctgacgcc tgatctgacc aagaagctcg gctatgaata
     3901 tctgcgcgac ttcatcacct acggttcacc cggcggcatg ccgaattggg gaagctcggg
     3961 ccagctctcc gagaacgaag tcgccttgat ggcgcgttat ctcctcaacg aaccggcgca
     4021 gccgccagag ttcggtctca aggaaatgaa ggagagctgg aaagtcctgg ttccggtcgc
     4081 gcaacggcca accaagaaaa tgaacaagct cgacatcgac aatctgtttt cggtgacgct
     4141 gcgcgatgcg ggcgaagtgg ccttgatcga cggcaacacc aagaaaatcg aatacattct
     4201 gcagtccggc tatgccgtgc acatctcgcg catgtcggct tcggggcgct atctctacat
     4261 catcgggcgc gatgcgaagc tcaacgtgat cgatctctgg atggatccgc cgcagacggt
     4321 cgcggagctg aagatcggaa ccgaggcgcg ctcggtcgag acctccaagt tcaagggcta
     4381 cgaggacaga tatgcgatcg ccggagccta ctggccgccg caatatgtgc tgatggacgg
     4441 cgcgacactc gagccgctca aggtcgtctc gacgcgcggc atgacctacg acacgcagga
     4501 atatcatccc gagccgcgcg tcgcatccat cgtcgcctcg cattac
//
LOCUS       DATLJT010001761         5603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4572123,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001761 DATLJT010000000
VERSION     DATLJT010001761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4572123"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(565..891)
                     /locus_tag="VM910_39885"
     CDS             complement(565..891)
                     /locus_tag="VM910_39885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68688.1"
                     /translation="MTDNDKPDSNSRYWRYQAVRVDYPAYPDDPEYLVIEVCLSRANN
                     RLCSWSAEPASKRPSGDTYAELLDDLMRMLQDAREWKPVALDDLRVGMQFERAEVTDS
                     ARRGFE"
     gene            complement(988..1449)
                     /locus_tag="VM910_39890"
                     /pseudo
     CDS             complement(988..1449)
                     /locus_tag="VM910_39890"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2293..2820
                     /locus_tag="VM910_39895"
     CDS             2293..2820
                     /locus_tag="VM910_39895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68689.1"
                     /translation="MNARWILKWLGDKGQRVGLLILLVGIIALYFSIRGYYAQQSVNL
                     PNLVSIGPKIYSNNQTASIGLEWFNTGRKATIGGRALLFAVNKDQTRRHKIGETLISG
                     GLPGNAITARISVDMRQSLELFLVCVAYLDDNNKSYQQVYLYRLGRPTDGPDEIPLVE
                     ELRLGPPRAEICNEQ"
     gene            2908..5001
                     /locus_tag="VM910_39900"
     CDS             2908..5001
                     /locus_tag="VM910_39900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68690.1"
                     /translation="MLKGFLGLIAIALSPLIAAAGEEEIIVETSHATIESQDGQPLRV
                     VFAAEERPALIFKPRAGIWDWSQSRIAIPVENPGDEALTLLLRVDDEMSRSKTGQVGI
                     APRSSGSLVISISAPQPRSMGMIAGPSPAGAGLDPDTLPVTATDGAIDGSHVTFVQLG
                     MLRPLAPKHLIVGPPRVEPDRTSFDGMVDGFGQFFPGEWPEKVSSAEILRAKGAEEAR
                     TVARWLADSPKRDRFGGLLEAGSFRATGFFRTEQRDGRWWLVTPEGNGFFSIGMDVIA
                     LAGDTYVEGREFMFRDLPAPDGEFAAHWSERDDRGGLWPQPGRAFNHGHAFNFYTANL
                     QRKFGADWRERWREETVRRLEAWGFNTIGNWGEPDLWAMHRLPYTVPLWLEGKFWWGG
                     IPDPFDPRFVIAVDKIAENAAARFGGDAWLVGYFVDNELAWRRGWSTDPRERFALAIS
                     TLGRGAESPAKSAFVAQLIEIYREPERLAEAWGIPINSWDELRAAGFALPLESTGKPA
                     VIRDLAAFTRRYAEGYFRTVAEALHRHDPNHLYLGSRFAWRTPEAIEACARWCDIVSF
                     NLYQRSIDKDNDEWARFHALGKPALIGEFHFGSTDRGLFWEGLVGVGRESERGAAYSR
                     YLRTVADNPDFVGAHWFQYIDEPLTGRTLDGENAHVGFVTVADLPYAGLVAAAREANL
                     SVLRELRRIAVRATQ"
     gene            5337..>5603
                     /locus_tag="VM910_39905"
     CDS             5337..>5603
                     /locus_tag="VM910_39905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68691.1"
                     /translation="MESRKTNIAVEDRFKVVPFEEFCEIAHVGHSRGYEFLNSGDIKS
                     VLVGRRRLVLIQSWYDYLERLLEEQRTFTPGRHPASRQSPPSEPA"
BASE COUNT         1104 a         1650 c         1720 g         1129 t
ORIGIN      
        1 ggttgctgac gtggcaccaa atgccgacgg agagaatggg cccgccgccc ggagcttcgc
       61 cgccgatgcg catgactgca tcatggttag gatccaaacg gacctgccgc atacaagttt
      121 ggcggcgcga agctgcgccc caatggcgag ctcccctcgg attaataccc cggccaagcg
      181 ttgtccgacg ccgcagcttc atagaactcc tctcactggt catcatccct ccgcgtgctc
      241 aggtgtcaaa gcctgcccct ggcttgaaca gggggcgctg cgaaaaacac aagcacgcta
      301 agcggcaagc cgctaagctg cgctagcatt tcggtccctt gacacctttc cgcgcgctcc
      361 gcgaacctga tcgcaggcga cgggacgagg aacggccttt ccgctccaaa caaggaaccg
      421 gcaacaacca aaaaccactt gacagaaaaa ctcccataca agtccgcaaa acttcagtct
      481 ctctccgccg tctcccaaat tggggcgagg cgttccttgg cgacaacgcc gttgctcatt
      541 cgaacggttc gcagatcgct ccccctattc gaatccccgg cgagcgctgt ccgtgacttc
      601 ggcgcgctcg aactgcatgc cgacccgcag gtcgtcgagc gcgaccggtt tccactcccg
      661 cgcgtcctgc agcatccgca tcaagtcgtc gagcagttcc gcgtatgtgt cgccgctagg
      721 gcgtttgctg gcaggctccg cgctccatga gcagagacgg ttgttggcgc gactgaggca
      781 cacctcgatg acgaggtatt cgggatcgtc cgggtacgca gggtaatcga cgcgtacggc
      841 ctgatatcgc caatagcgcg aattggaatc gggtttgtcg ttgtcggtca tgctgtgggc
      901 tccaatttga tgcggtctcg ccgatcagtg tgtcgatcgt agcgattgag cagccagctt
      961 cggagcagga aatcacccac ttgcgagcta atagcgacag ctggcgacac ccgctgcttg
     1021 caccagcggc ctcgaggata gtgagccgca cccctcagtg cgcgccgcct cccgcgcgga
     1081 tgcaatgttg ggctcggcat tcgtattctt cgggttcacc agcagggcga tgaccccagc
     1141 ctgcggaacc agctcggaaa gcagttcaag gcgtttcggc gtcagctcgt cggcgagcaa
     1201 gctgaccccc gtgaggttgc cgcccggcct ggcgaggctg gcgacgaggc ctaactcgac
     1261 cgcgtcggcg ccaatcctga agacgatcgg gatcgtcgag gttgcgcttt tcgccgcgaa
     1321 tgccgtaggg gcgccgcccg tggagacaat cacgtcaacc ttgcggccga cgagctcggt
     1381 ggccaatgcg ggcagccgat catagtggcc cttcgccccg cggtattcga tcgccacgtt
     1441 ctgtccctcg acatagcctt tcgttcagtc cctggcgcaa cgcggccaca tacggtgcaa
     1501 acgctccggg cgaggccgtg ctgaggatgc cgatcaccgg catcgccctc tgctgcgcaa
     1561 ggagggggca agccaccgcc gtcgtaccgc ccagcagcag cagcaactcg cgccgcctca
     1621 tctgtcgacc gcccctgtgc tgttcaccct gtccccggtc ttcattgccc gtcctcgacg
     1681 gctcggcagc taggcgcagt ttaaccgcgg ggtgcgcgtt ttcacatctg cagctggacg
     1741 gagaggcagg ctcggcggcc ccgagcagtt accgagagaa agcgcctttc gcgaactgtc
     1801 cgtcatagga tgggccgcac accagcctcg gcggtctggc cccgaacgag tttgcagcct
     1861 ggtctcaacg ggaccacaac cggagcggct tctggttatc agtgagggca agcaaggggc
     1921 ggggtcaatg atgaaggcct cgggcgtggg aacatcctcg ggttgcgaca gtcagacgaa
     1981 tcgttgacgg cgttattgac gcgagtatgg ttaacaactc tctttgatta ggggcgcgat
     2041 gtgagcgttt atagcacctc aaaaaaagcg gacccgcgga taggaggaga gctgatagat
     2101 gggcaatccg gcctcggggt gcgcttgcct ctccgatgat cggcaaatag cgaacttcga
     2161 ctaaagtccc ggcgtgtcag gctcccccag tgttatccgg ggcatgttcg ttcagtgtag
     2221 tcgctggaag agcgaggtcg ggactggcta tagacggcaa cctgagaagt gcatggacgt
     2281 agaggggaag cggtgaatgc gcgctggatc ttaaagtggc taggggacaa gggtcaacga
     2341 gtcggccttt taattctgtt ggttggcatt attgctctgt attttagcat ccgtggttat
     2401 tatgcgcaac aaagcgtcaa cttgcctaat cttgtttcta tcgggccaaa aatatattct
     2461 aacaaccaaa ccgcctccat cgggctagaa tggttcaata ccggaagaaa ggcaaccatc
     2521 ggcggtagag ccttgttatt tgctgtcaat aaagatcaga ccagacgaca caagattgga
     2581 gaaacgttaa ttagcggggg ccttcccgga aatgctatca cagcaagaat tagtgtagat
     2641 atgcgtcaat cactcgagtt atttctcgtg tgtgtggctt accttgacga taacaacaaa
     2701 agctaccagc aagtttatct ctatcgtctc ggtaggccaa ccgatggacc cgacgaaatc
     2761 ccgctcgtcg aggaactgcg cttgggcccg cccagggccg aaatctgtaa tgaacaatag
     2821 tggaatccgt ccaggcgctc ggaaccgcgc cttgtgatat cctccatact gctgcgatag
     2881 ctgcacaaac ccggcgagcg ggctacgatg ctaaaagggt ttctcggttt gatcgcgatc
     2941 gccctctcac cgctgattgc agcagcgggc gaagaggaaa ttattgtgga aacatcacac
     3001 gccaccatcg agtcacagga cgggcagccg cttcgggttg tgtttgcggc ggaggagcgg
     3061 cccgcgctga tctttaagcc gagggctggc atctgggatt ggtctcagag ccggattgca
     3121 atcccggtcg aaaatccggg agacgaagcg ctgaccttac tattgcgtgt cgacgacgag
     3181 atgagccggt caaagactgg tcaggtaggg attgcgccgc gcagctccgg cagcctcgtc
     3241 atctcgatca gcgcgccgca gccacggtcg atgggcatga tcgccggacc ttcgcccgca
     3301 ggtgcgggcc tcgatcccga caccctaccc gtgacggcga ccgacggcgc gatcgacggc
     3361 tcgcatgtta cgttcgtcca acttgggatg ctgcgtccgt tggcccccaa acatctcatc
     3421 gtcggaccgc cgcgcgtcga gccggatcgc acctcctttg acggcatggt cgacggtttc
     3481 ggccagttct tcccgggtga gtggccggag aaggtgagtt cggccgaaat tctccgtgcc
     3541 aaaggtgccg aagaggcgcg cacagtcgcg cggtggcttg ccgactctcc gaagcgcgat
     3601 cgcttcggtg ggcttctgga ggccggcagc tttcgcgcaa ccggcttctt ccgaaccgaa
     3661 cagcgtgacg ggcgatggtg gcttgtcacc cccgagggca atggcttctt ctcaatcggg
     3721 atggatgtga ttgcgcttgc tggagatacc tatgtcgagg gccgcgagtt tatgtttcgc
     3781 gatctgccgg cgcccgatgg agagtttgcc gcgcactgga gcgagcgcga cgaccgtggc
     3841 ggtttgtggc cccagcccgg ccgcgcattc aatcacggtc atgccttcaa cttctacacc
     3901 gcgaacctgc agcgcaagtt cggcgctgat tggcgcgagc gctggcgcga agaaacggtg
     3961 cggcggctcg aggcttgggg ttttaacacc atcggcaatt ggggcgagcc cgatttatgg
     4021 gcgatgcatc gcttgcccta cactgtgccg ctatggctag agggtaagtt ttggtgggga
     4081 ggaataccgg acccgttcga tccgcggttt gtcatagctg tggataagat agccgagaat
     4141 gccgccgcgc ggtttggggg cgacgcttgg ctggtcggct atttcgtcga caacgagctg
     4201 gcttggaggc ggggctggtc gaccgatcca cgagaacgtt ttgccctggc gatcagcacg
     4261 ctgggccgcg gtgcggaaag cccggcgaaa tcggccttcg tcgcgcaact catcgagatc
     4321 tatcgcgaac cagagcgact cgcggaggct tggggcattc caatcaactc ttgggatgag
     4381 ctgcgcgccg cgggtttcgc cctgccgctc gagagcaccg gcaagcccgc ggtgatccgc
     4441 gatctagcgg ccttcacccg gcgttatgcc gaaggttatt tccgaacggt agctgaagcg
     4501 ctgcaccgtc acgatccgaa ccatctctat ctcggcagcc gttttgcgtg gcggacccct
     4561 gaggcgatcg aggcgtgcgc ccgctggtgc gatatcgtca gcttcaatct ctaccaacga
     4621 tcgatcgaca aagacaacga tgagtgggcg cgtttccatg cgctcggcaa accggctctg
     4681 atcggcgagt ttcattttgg ctcgaccgac cgcggactat tttgggaagg tcttgtcggg
     4741 gttggcagag agagcgaacg cggcgccgcc tactcccgtt accttcgcac tgtcgccgac
     4801 aaccccgatt ttgtcggcgc ccattggttc caatacatcg acgaaccgct gaccgggcgg
     4861 accctcgacg gcgaaaacgc gcatgtcggc tttgtcacgg ttgccgatct gccgtacgcg
     4921 ggccttgttg ccgcggcacg cgaggccaac ctgtcggtgt tacgcgagct acggcgtatt
     4981 gcggtccgcg ctacacaata actgacaatg gcgagttggt aagttcagaa gaatttcgcg
     5041 aaggggaagc gagcggtccg ctggacaatg actcgggatg gttacggatg cggcggtgct
     5101 cacagacagc tatcgccgcc cgtcccatag catctctccg ccggagggag atggattcga
     5161 acttcgagct tctccgaccg tcagccaatg caccggagag atcatgcgcg agaccgattg
     5221 cgaagtggtg gttgccgagt catccgtacc aaggggcgcg acacctatcc gcggacgtgt
     5281 ggtgatgccc cggcgtgatg acgtcatgat agctcttttc atcaagaggc cgcttagtgg
     5341 agagccgcaa gacgaatatt gctgtagaag atcgcttcaa agtcgtacca tttgaggaat
     5401 tttgcgaaat cgcacatgtt gggcatagca gaggctacga gtttctaaat tcgggcgaca
     5461 ttaaatcggt tttggtcgga cggcgccggc tggttcttat tcagagctgg tacgattatc
     5521 tcgagcggct gctcgaggaa cagcgcactt tcacgcccgg ccgacatccg gccagccggc
     5581 agtcaccacc atctgagccc gcc
//
LOCUS       DATLJT010001762         1684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1587352,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001762 DATLJT010000000
VERSION     DATLJT010001762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587352"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VM910_39910"
     CDS             <1..787
                     /locus_tag="VM910_39910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI68692.1"
                     /translation="RVGLTAVLHTWGSALTHHPHVHVIVPGGGLSLDGERWVACRSGF
                     FLPVRVLSRLFRRLFLEGLAVLHEANRLAFFGDLAPLADQRAFDAALAPLRRSEWIVY
                     AKRPFAGPQAVLVYLARYTHRVAISNSRLIAFNEAGVTFNWKDYRIKGRDRLRTMTLD
                     GAEFIRRFLLHVLPSGFHRIRHYGLFAGTVRARNIERVRQALAAPKLSPTSAHVEADS
                     ETETPSSAPRCPCCGGRMIIVETFEGARPARSPPSSRIRIDTS"
     gene            1344..>1684
                     /locus_tag="VM910_39915"
     CDS             1344..>1684
                     /locus_tag="VM910_39915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HVI68693.1"
                     /translation="MSETRKLAAILVSDIVGYSRLAGADEDRVLARLRTLRSDLIDPT
                     IAVHHGRIIKRTGDGSIIEFRSVVDAVNCAIEVQRAMVERNAEVAPDKRIEFRVGIHL
                     GDVVEESDGDL"
BASE COUNT          290 a          629 c          467 g          298 t
ORIGIN      
        1 gcgcgtcggc ctcaccgccg tcctccacac ctggggctcg gcgctcaccc atcacccgca
       61 tgtccacgtg atcgttccgg gcggcggcct ttctttggac ggcgaacgat gggtcgcttg
      121 caggtccggc ttcttcctcc ccgtgcgcgt gctctcgcgc ctgtttcgtc gcctcttcct
      181 tgaaggcctc gccgtcctgc atgaggccaa tcggctcgcc ttcttcggcg atctcgcgcc
      241 ccttgccgac cagcgcgcct tcgacgccgc gctcgcgcct ctgcgtcgat ccgagtggat
      301 cgtctacgcc aagagaccct tcgccggacc gcaagcggtt ctcgtctatc tcgcgcgcta
      361 cacccaccgg gtcgcgatct ccaactcccg gctcatcgcg ttcaacgaag ctggcgtcac
      421 cttcaactgg aaggactacc gcatcaaagg ccgcgaccgg ctcaggacca tgacgctcga
      481 cggcgccgag ttcatccgcc gctttctcct gcatgtcttg ccgagcggct tccaccgcat
      541 ccgccactac ggcctgttcg ctggaacggt tcgagcgcgc aacatcgagc gcgtccgcca
      601 agcgctcgcc gcgcccaagc tctcgcccac gagcgcgcac gtcgaagccg acagcgagac
      661 cgagacacct tcgtctgcgc cccgatgccc gtgttgcggc ggccggatga ttatcgtcga
      721 gacgttcgaa ggcgcgcgcc ctgcgcgttc cccaccgtca agccggatca ggatcgacac
      781 ctcatgatga tcgtcgcggc gcctcccgcc tcgcgccacc gtttcccccc gtctccgacc
      841 gcgcgccgga gcaggaagcc gatgtcctca caacgtcgcc actcctcctc cgaccgccat
      901 gcctgcgcga ggcgcgctca tcctcgcgat cgaaaacgcc agccgtcgac gttcccgccg
      961 ccaaaaccgc acaccacccg ccatcgcggc gaagcacgcc agccgtccgc gatccccaaa
     1021 tccccatagt tcgcgcccga acaaaacagc gccctaccta cccgcggttt cctccttggg
     1081 aggctttcga acgccggccc cacatcgcgc acgaccgtcc cggaaggggc cggcatccga
     1141 aacccttcac cgaagcacgc actcccgagt tggaccctgt acgggcggaa agtgcgagaa
     1201 agcggacttt ggctaaggcg tgtccctctc ttgaaagaca aaggaattca ttcattcaat
     1261 ctgcttcggc atttaaagct ccccgcattt gggcacaccc gggctggcat ttgtgctaat
     1321 gcgccaacag tcggatgggc gcaatgagcg agacccggaa gctcgcggcg atcttggtat
     1381 ccgacatcgt cggctactcc cggcttgctg gcgcggacga agatcgcgtc ctcgcacggc
     1441 ttcggaccct tcgcagcgat ctgatcgacc cgaccatcgc ggtccatcac ggtcgcatca
     1501 tcaagcgcac tggcgacggc agcatcattg agttccgcag cgtcgtcgat gcagtaaact
     1561 gcgccatcga agtccaaagg gcgatggtcg agcgcaacgc cgaggtcgcg ccagataagc
     1621 gcatcgaatt tcgtgtcggc atccacctcg gcgacgtggt cgaggagagc gacggcgacc
     1681 tcat
//
LOCUS       DATLJT010001763         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2047888,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001763 DATLJT010000000
VERSION     DATLJT010001763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047888"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1183
                     /locus_tag="VM910_39920"
     CDS             128..1183
                     /locus_tag="VM910_39920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68694.1"
                     /translation="MKNRTREICTSGSVRDEDGQPPHLLGRRNFLHLAAGAGALLAVS
                     RVALAQTYPSRPITMIVPFPAGGPTDTIGRIVAEGMRVSLRQPIIIENVTGAGGTIGV
                     GRVARAVPDGYTLSVGFLGTHVLNGAIYTLQYDVLKDFEPVALLASNPQLIVAKNATP
                     AKNLNELIVWLKGNPGKASQGTAGVGSPAHVSGAYFQKETGTRFQFVPYRGAAPAMQD
                     LVAGQIDLMFDQAVNSLPHVRGGKIKAYAVTANTRLASAPEIPTVDEAGLPGFYVSIW
                     SGMWAPKGTPKDVVAKLNAAVVDALAHPTARQQLADLGQEVPQRDQQTPEALGVFQRA
                     EIEKWWPIIKAAGIKAE"
     gene            1688..>2170
                     /locus_tag="VM910_39925"
     CDS             1688..>2170
                     /locus_tag="VM910_39925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68695.1"
                     /translation="MLNMNRREFITLLGGAAAAWPLAVQAQLHPKMLRVGFVGMQPRE
                     SPLYANFLKRMAELGYQEGRNFTFDYIQTPNIEGYEKDYRELAARKVDVFLAVGNEPA
                     LRAGFQSRRGLSHDDSAPVTPHNAPFQTYKAAGVHHPSRRRGSRLAARGACAAAGDEK
                     G"
BASE COUNT          439 a          650 c          659 g          422 t
ORIGIN      
        1 aatggctgtc gcggcgggat cgcgggagcc ggctccactg gcgccggttc actgcactcc
       61 tcaagcgctg ccctctgcca gcgcctcgaa ttgtccatcg gtacaccacc acgagcgagg
      121 ctctcccatg aagaaccgga cgcgggaaat ctgcacatcc gggtctgtga gggatgagga
      181 cgggcaacct cctcatctac tcggccgccg aaattttctg catctggccg caggcgctgg
      241 cgcgctcctg gccgtctcgc gagttgcttt ggcgcaaacc tatccatcgc ggccgatcac
      301 gatgatcgtg ccgtttcctg ctggcggccc aacagatacg attggacgta tcgtggccga
      361 gggcatgcgg gtctcgctcc gccagcccat cattattgaa aacgtaaccg gcgccggcgg
      421 tactatcggc gtgggccgcg tcgcgcgtgc ggtacctgat ggctatactc tcagcgtcgg
      481 cttcttgggc acgcatgtcc tgaacggcgc tatctatacg cttcaatacg acgtactgaa
      541 ggatttcgag ccagtcgcgt tactcgctag caatcctcaa ctaattgtcg caaagaacgc
      601 tacacccgcg aagaatttga atgaattgat cgtgtggttg aaagggaacc caggcaaggc
      661 gtcgcaggga accgcaggcg tgggcagccc cgcgcacgtc agtggcgctt acttccaaaa
      721 agagacgggc acgcgctttc aattcgtacc ctatcgcggt gccgctccgg ccatgcagga
      781 tttggtcgct gggcagatcg acctcatgtt cgaccaagct gtgaactccc ttccgcacgt
      841 gcgtggcggc aagatcaaag cttacgccgt aacggccaat acccgcttag cctcggcgcc
      901 tgaaatcccg actgtggacg aggccggact tccggggttt tacgtttcta tctggtccgg
      961 catgtgggcg cctaaaggca cacctaagga cgtagtcgct aagctcaatg ctgcggtcgt
     1021 ggatgcccta gcccacccga cagcgcggca gcaacttgcc gaccttggtc aggaggttcc
     1081 gcagcgcgac caacagaccc ccgaagcgct aggtgttttt caaagggccg aaatcgagaa
     1141 gtggtggccg atcataaagg cggcaggcat caaggcggaa tgaatagcgt cgcccgagcg
     1201 gaccaaggct gcgattgcgg agcggcagaa attcagcgca ttgctgaaaa tgctgccggc
     1261 tggcctggca tgaaatacgt aaagtcgaaa ggggcgtgga gttttgcagc cctcctcaac
     1321 atggccgtcg agccgctcgc catgtgctat cctgctctgc ttggaggcac ttgagagaca
     1381 ggccaacacc tcgattcaga acaattcaga tctgccccaa ggacctgccc atccgttcgg
     1441 tggcaggctg agctgcgggc gggatcatgc cctgctgttt gtcaagtagt ttcggaccct
     1501 aacgaggtca tccaccatga gcgagcaggc tgtagaggag aggcgaggtt gggcttccga
     1561 catcgtaggg gtcgtggtct attcgatcct ggttctcctg gtcatgcttt tgttaataga
     1621 cgttccggcc taaggccacg gcggcaaacc ggctagggaa agccggacgc accgaggaga
     1681 aaaccccatg ctcaacatga accggcgcga gttcatcacg ctgctcggcg gggcggccgc
     1741 cgcgtggccg ctcgcggtgc aggcgcagct gcatcctaag atgctgcgtg tcggctttgt
     1801 cggtatgcag cctcgagagt cgccacttta tgcaaatttc ctcaagcgga tggccgaact
     1861 cggctatcag gaaggtcgga actttacctt cgattacatc caaacaccga atatcgaagg
     1921 gtacgaaaaa gactatcgcg agctcgcggc gcgcaaggtt gacgtgttcc tggcggtcgg
     1981 aaacgagccg gcgctgcgcg cgggcttcca gtcacgacgg gggctgagcc atgacgactc
     2041 ggcccctgtc acccctcaca atgctccttt ccagacatac aaggcggcgg gcgttcatca
     2101 tccttctcgg cggcgcggca gccgcctggc cgctcgcggt gcgtgcgcag cagccggcga
     2161 tgaaaaaggc
//
LOCUS       DATLJT010001764         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3888200,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001764 DATLJT010000000
VERSION     DATLJT010001764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3888200"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1017)
                     /locus_tag="VM910_39930"
     CDS             complement(43..1017)
                     /locus_tag="VM910_39930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HVI68696.1"
                     /translation="MTLARFLPRLALALLLVAAAAWVAVHRDRIDLATLDGWLGSLGL
                     WAPVGYIALYALATVALVPGVVFSLAGGALFGPFWGSILNLMGGTLGATLAFLAARYI
                     GGDWVTRKAGGLLKRLIDGVDAEGWRFVAFVRLVPLFPYILLNYALGLTHIPFQHYVI
                     ATFVCMAPAAVAYTWLGHAGRGALAGDTDAVWYGLLALGLLAAIVLLPRLIKRLRGKF
                     DWIEANELKRRLDRGEAVAIIDVRGRDEFVGPLGHIATARNIPVAELESRLSELAGLE
                     RGPIVLVCRTDKRSATAARTLRAAGFTQVNVLRRGMEEWNETRLPVQA"
     gene            complement(1017..1856)
                     /locus_tag="VM910_39935"
     CDS             complement(1017..1856)
                     /locus_tag="VM910_39935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HVI68697.1"
                     /translation="MRDALFVYEPLVRLAAFAGVFGVMAVWELLTPRRRQAIGRNWRW
                     PNNLGVLAVDVVLVRILLPITAVGLALIAEARGFGLLNLIALPRWASIIISVILLDLA
                     IYLQHVLFHAVPALWRLHRMHHADLEFDVTTGVRFHPIEILLSTAIKLTVVAALGTPA
                     AAVVIFEVLLNATSMFNHSNVRVPAQIDRVLRWFVVTPDMHRVHHSILRRETNSNFGF
                     NLPWWDRLLGTYRAQPAAGHEGMTIGIEQFRDARELGLDRMLLQPFRGEAGRYPIAAR
                     EAD"
     gene            1933..2601
                     /locus_tag="VM910_39940"
     CDS             1933..2601
                     /locus_tag="VM910_39940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVI68698.1"
                     /translation="MSRSNPKLTLFAQFAAVAKALAHAHRLEILEQLAQGERAVEVVA
                     QRTGLSVANTSQHLQHMRRAGIVAARREGKFILYTLADEAVLDLMATLRRIAQRNVAE
                     VERVIRSYFNDRDSMEPVSREQLLENSRAGVVTVLDVRPADEFALGHLPRAVNIPLRE
                     LEARLGELDRDQEIVAYCRGPYCVLSYEAVASLRARGFKARRLEDGLPEWRAAGLPIV
                     TGSA"
     gene            complement(2707..>3358)
                     /locus_tag="VM910_39945"
     CDS             complement(2707..>3358)
                     /locus_tag="VM910_39945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVI68699.1"
                     /translation="KSIKADETAILLVDFQANFVSPDGAWYGKFAEHYKKTRMLDRIV
                     ELVRKARAKGVLVVHITEGYTSDYRELDPSNPGMFHRGQIGRGAWKVGSKEAAYYEPL
                     KPAPGDRDLFLPPRSQASGFGGTGLSEILRAKGIKNVAVAGFTSDVCVYATVLSAYDL
                     GFHVYALREGMVGFFDGMAEQMVNFVYPMWSQVIDNDEFDNMIEASPAASRKASAN"
BASE COUNT          677 a         1054 c         1063 g          564 t
ORIGIN      
        1 tatgcccttg gcggcggtcg ccgcggttgt aggctgtgcg gcctaggctt gcaccggcag
       61 gcgggtctcg ttccactcct ccatgccacg gcgcaacacg ttgacctgcg taaagcctgc
      121 ggcacgcaag gtccgcgccg ccgtcgccga gcgcttgtcc gtgcggcaaa cgagaacgat
      181 cggtccgcgc tcaagcccgg caagctcgga taaccggctt tcgagttcgg ccacgggaat
      241 attgcgcgcc gtcgcgatgt gaccgagcgg cccaacgaat tcatcgcgtc cccggacatc
      301 gatgatggcg acggcttcgc cgcgatcgag ccggcgcttg agttcattcg cctcgatcca
      361 gtcgaatttg ccgcgcagcc gtttgatcag tcgcggcagc aagacgatgg cagcgagcaa
      421 gccgagcgca agcaaaccgt accacacggc atcggtgtcg ccggccagcg ccccgcgccc
      481 ggcgtgcccg agccaggtat aggcgaccgc agcgggagcc atgcacacga aggtggcgat
      541 aacgtagtgt tgaaacggaa tatgcgtcag cccaagcgcg tagtttaaca gaatataggg
      601 gaaaagcggg accagtcgta caaacgcgac gaagcgccag ccctccgcat cgactccgtc
      661 gatcagtcgc ttgagcaggc cgcccgcctt gcgggtgacc cagtcacccc cgatgtagcg
      721 cgcggcgaga aatgcaagcg tggcaccgag agtgccgccc atgaggttta agatgctgcc
      781 ccagaatggt ccgaacaacg caccgccggc tagagagaaa acgacgccgg gaacaagagc
      841 aactgtagcg agggcataga gagcgatgta gccaaccggt gcccagaggc caagtgaacc
      901 gagccaacca tcgagtgtcg caagatcgat cctatcccga tgaacggcga cccacgccgc
      961 cgctgccacc agcagcagtg ccagcgcaag tcggggtaag aaacgagcga gagtcattaa
     1021 tcggcctccc gcgctgcgat cggatagcgg cctgcttcac cacggaacgg ttgtaatagc
     1081 atgcgatcga ggccgagttc gcgggcatca cgaaactgct cgataccaat tgtcatgccc
     1141 tcgtggccgg ccgcaggctg ggcgcggtag gtgcccaaca gacggtccca ccaaggcagg
     1201 ttgaagccga aattgctgtt ggtctcgcgc cgcaggatcg aatgatggac gcgatgcatg
     1261 tccggcgtca cgacaaacca gcgcagcacg cgatcgattt gcgcaggaac gcggacgttg
     1321 ctgtggttga acatcgaggt cgcattgagc aggacctcga agatcactac ggccgccgcg
     1381 ggcgtcccga gtgccgcgac gacggtaagt ttgatcgcag tcgacagcaa aatctcgatg
     1441 gggtgaaaac gcaccccggt ggtcacgtcg aactcgaggt cggcatgatg catgcgatgc
     1501 agccgccaga gcgccggcac ggcatgaaag agcacgtgct ggagatagat cgcgaggtcg
     1561 aggaggatga ccgagatgat gatgcttgcc caccgcggca gcgcgatcag gttgaggaga
     1621 ccaaatccgc gcgcctcggc aatgagcgca agaccgacag ccgtgatcgg caacagtatg
     1681 cgcacgagca ccacgtcgac cgccagcacg ccgaggttgt tcggccagcg ccaattgcgt
     1741 ccgatcgctt gtcgcctgcg cggcgtaagg agctcccaaa ccgccatcac cccgaacacg
     1801 ccggcaaatg cggccaagcg aaccagcggt tcgtatacga acaaggcatc acgcatcggt
     1861 catcggcttg acaaaggctt ggattcgcgg ttattcaatc attcacttga atgatgttgg
     1921 attgctcgcc cgatgtcaag gtccaacccg aagttgacgc tgttcgcgca gttcgctgcg
     1981 gtcgccaaag ccctggccca cgcgcatcga ctggagattc tagagcagct ggcgcaaggc
     2041 gagcgcgccg tcgaggtggt cgcgcaacga accggcctct ccgtcgccaa cacgtcgcaa
     2101 cacctgcagc acatgcggcg cgcgggcatc gtagcggcgc gacgcgaggg aaagttcatc
     2161 ctctacacgc tcgccgatga ggctgtgctc gatctcatgg caacgctgcg ccggatcgcc
     2221 cagcgcaatg ttgccgaagt ggagcgcgtg atccgcagct acttcaacga ccgtgacagt
     2281 atggagccgg tctctcgcga gcaactcctg gaaaattcgc gcgccggcgt ggtcaccgtc
     2341 ctcgacgtcc gcccggcgga tgaattcgcg cttggacatt tgccgcgtgc agtgaacatt
     2401 ccgttgcgcg aacttgaagc gcgcctcggg gagctcgatc gcgaccaaga gatcgtcgcc
     2461 tattgccgcg gtccgtactg cgttctttcc tacgaggcgg tggcctcgct tcgcgcgcgt
     2521 ggcttcaagg cgcgccggtt ggaagatggc cttccggagt ggcgcgctgc ggggctgccg
     2581 atcgtcaccg gcagcgcttg acccggacgc aagcgcctat cgccaacgga cggccttgac
     2641 gatggcatcc atgcccgccg ccagcacaag cggcgatccg aagcgtctcc gacatggtcc
     2701 ggttcgttag ttggccgacg ctttgcgcga cgccgccggg ctcgcctcga tcatgttgtc
     2761 gaactcgtcg ttgtcgatga cctgcgacca catgggatag acgaagttga ccatctgctc
     2821 cgccatcccg tcgaagaagc ccaccatgcc ctcgcgcaac gcatagacat ggaagccgag
     2881 atcgtaggcc gacaagacgg ttgcgtagac gcacacgtcg ctggtgaagc cggccacggc
     2941 gacgttcttg atgcccttcg cgcgcaggat ttcgctcaat ccggttccgc caaacccgct
     3001 ggcttggctg cgcgggggca gaaacaaatc gcgatctccc ggcgccggct tcagcggctc
     3061 gtagtaggcg gcttccttcg agccgacctt ccaggcgccg cggccgatct gcccgcggtg
     3121 gaacatgccg ggattggaag gatcgagctc gcgatagtcc gaggtgtagc cctcggtgat
     3181 gtgcacgacg agaacgcctt tggcccgcgc cttgcgcaca agctcgacga tacggtcgag
     3241 catgcgggtc ttcttgtagt gttcggcgaa cttgccgtac caggcgccgt ccggcgaaac
     3301 gaaattggcc tggaagtcga ccagcaggat ggcggtctcg tctgccttga tgcttttg
//
LOCUS       DATLJT010001765         1779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_210187,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001765 DATLJT010000000
VERSION     DATLJT010001765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210187"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(882..>1779)
                     /locus_tag="VM910_39950"
     CDS             complement(882..>1779)
                     /locus_tag="VM910_39950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI68700.1"
                     /translation="PHHCTREFMIACRDIAREHDLAMQTHVSEAKYQGVSTFKMFGKS
                     TVEVMDACGLVGPWFSAAHAIWISDTDMDLLSRKGAQVSHNGAANLRLGSGIAAVREY
                     IRHGVTVGIGTDGSASSDNQNMFEAMRAAAFVSRVRGLPPADWVSTAEAFIMATEGSA
                     RVLGMGDFIGRIAKGYKADLVLLDLDHVNFVPLNNATNQIVFTENGGAVDKVLIGGKL
                     VVTGGRAIGIDRAALAAKAQAAVERLAALNADARRFAERIEPIVSNFCRGLADDDMMH
                     RLRRDLGRPDARCVHCGKPPHG"
BASE COUNT          322 a          546 c          574 g          337 t
ORIGIN      
        1 ggtctgctcg accaccatcg ggacgagctg gttctggtag gtatcgcgcg gatctctcac
       61 gccgcctcct ttagacatgc cgatcgccat cagcgcaaat ttcttgcgat ttgaactcgt
      121 catcaacctg aagacggcaa aagatattgg acacgaagtc ccgaagggtc ttccgctttg
      181 acggcttctg agcgcgcctg catcgcttcg acggccgcgt ggtcgttcgg atcttcatcg
      241 cgtgacggaa gtcgcggtat ccggttacgc cttgggcatc ggcgtttcgt aagcggcgac
      301 tttgaccggc acttgcggcc gaccgggact gtcatgatcg atatatgtct cggtgaccgc
      361 gcccggcaat atccgcgtat gccgaaaaga gaggcaccta ttggattaaa aaaagagagg
      421 cacctattgg atttcggaaa acaatgtctg agcgtttccc ggcccacgcg agaaccattt
      481 gcggagccgg gcgtgaagaa cctccagcat aatttagaga agctaactcg ccggcttgaa
      541 gctgagttgc gcggctctcc acccgctcgg cgccagcgaa gcgaggatcc aggacgagat
      601 ccaatcgcgg tcccggacga cattgctgag aaagatccgg cgcgttgatc ggcggcgacg
      661 ccttacgcaa ctgtcgtggt gcaattcgca gcaaggcccc gtagcgcaat ttgcgctaca
      721 tgccgtcacc ggcagttgaa gtcacggggg cggaccgcca ggcgttctcg acctataaag
      781 cggcgtccat catgatgaaa attgcggttg tcggcgcgtg agctgcgcga gaccgtttca
      841 atgtctgagc gtttccacct ggcgctcgca ctcgccacgc cctacccgtg tggtggtttc
      901 ccgcaatgga cgcagcgggc gtcgggccgg ccgagatcgc ggcgcagccg gtgcatcatg
      961 tcgtcgtcgg cgagaccgcg gcagaagttc gagacaatgg gctcgatgcg ctcggcgaag
     1021 cgccgggcat cggcgttgag cgcggcgagg cgctccaccg cggcctgcgc ctttgccgcg
     1081 agcgcggcgc ggtcgatgcc gatggcacgg ccgcctgtga ccacgagctt gccgccgatc
     1141 agcaccttgt cgacggcgcc gccgttctcg gtgaacacga tctggttggt ggcgttgttg
     1201 agcggcacga aattgacgtg gtcgaggtcg agcagcacga ggtcggcttt gtaacccttg
     1261 gcaatcctgc cgatgaagtc ccccataccc agcacccgcg ccgacccttc ggtcgccatg
     1321 atgaaggctt cggccgtcga gacccagtcg gccggcggca ggccgcggac ccgcgagacg
     1381 aaggcggcag cgcgcatcgc ttcgaacatg ttctggttgt cggacgaggc tgagccgtcg
     1441 gtgccgatgc cgacggtgac gccgtggcgg atgtattcgc ggaccgccgc gatgcccgac
     1501 ccgagccgca gattggcggc gccgttgtgc gacacctggg cgcccttgcg gctgagcagg
     1561 tccatatcgg tgtcgctgat ccagatggca tgcgcggcgg agaaccaagg ccccaccagc
     1621 ccgcatgcgt ccatcacctc aaccgtcgat ttgccgaaca tcttgaaggt cgacacgccc
     1681 tggtatttcg cctcgctcac atgggtctgc atggccagat catgctcgcg ggcgatgtcg
     1741 cggcacgcga tcatgaattc gcgggtgcag tggtgcggg
//
LOCUS       DATLJT010001766         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2967612,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001766 DATLJT010000000
VERSION     DATLJT010001766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967612"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VM910_39955"
     CDS             <1..175
                     /locus_tag="VM910_39955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HVI68701.1"
                     /translation="KFAARARVTGDVGIYHLRGCSSYAGLTQPDRWFCTEEDAQAAGF
                     RRAFNCRAKPGAK"
     gene            complement(190..636)
                     /locus_tag="VM910_39960"
     CDS             complement(190..636)
                     /locus_tag="VM910_39960"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HVI68702.1"
                     /translation="MPTYTVTAPEGCLDQGKRARIAQEITRVHSETTGAPTYFAQVIF
                     NDVASGKYFVGGVPLRGKQVFVNGQIRAGRTAESKDRLIAQMLAAVSGAAEVPANHVW
                     VYITDLIPRQMAEFGNVLPEPGDEAGWTNALLPADRAHMESFRPAR"
     gene            complement(668..1495)
                     /locus_tag="VM910_39965"
     CDS             complement(668..1495)
                     /locus_tag="VM910_39965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI68703.1"
                     /translation="MKIVDAQVHIWSQTVVPTSGLHRKVEKFTAEEVLAEMDAAGVDA
                     ALIHPPGSWDPNANALAVDAARRYPDRFAVMGQFPLQDPENRKLIHGWRNQPGMMGLR
                     WPFLLEEQQKWLYDRSLDWLWPAAENEGLPIAMMGGLFLKKFREIAERHPRLRMIVDH
                     CGLNRRGRDAEAFIHLDELVALAGLPNVAVKATGAPHYSTQSYPFRNIQDGLHRIFDA
                     FGPKRFFWGTDITRMQCSYRQCVTFFTEELPWLGGGDLEDVMGRGLCQWIGWNYRFD"
     gene            1676..>2095
                     /locus_tag="VM910_39970"
     CDS             1676..>2095
                     /locus_tag="VM910_39970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68704.1"
                     /translation="MTGPEDDDGLAGKVALISGGGAAGDGIGNGRAAAILLARAGTKV
                     MVADREPALAERTVEMITHEGGTAAAIAGDVTLEADCRRLVEATVARFGRLDFLDNNV
                     GIGSRGSVVEEKPETYRRVMQVNVESMFLLSKYAIPAM"
BASE COUNT          375 a          688 c          655 g          377 t
ORIGIN      
        1 caagtttgcc gcgcgcgcgc gcgtgaccgg cgatgtcggc atctatcacc tgcggggctg
       61 cagcagctat gccggcctga cccaacccga ccgctggttc tgcaccgagg aggatgcgca
      121 ggctgcgggc tttcgccggg ctttcaattg ccgtgcgaaa cccggtgcga aatagggctt
      181 cgcacgcgtt caacgcgcag gcctgaaact ttccatatgc gcgcggtcgg ccggaagcag
      241 cgcattcgtc caaccggcct catcgccagg ctccggaagc acgttgccga actccgccat
      301 ctgccgcggt atcaaatcgg tgatgtagac ccagacatgg ttcgcaggca cttcggcggc
      361 gccggagacg gctgcaagca tctgcgcgat cagccgatcc ttgctctccg cggtcctgcc
      421 cgcgcgaatc tggccgttga cgaacacctg tttcccgcgc agcggcacgc cgccgacaaa
      481 atacttgccg gacgcaacgt cgttgaaaat cacctgcgca aaataggtcg gtgcgcccgt
      541 ggtttcgcta tgaacccgcg tgatctcctg ggcgatcctc gcccgcttcc cctggtcgag
      601 acatccttcc ggcgccgtca ccgtatatgt cggcatttcg catatcctcc tgtgttggca
      661 ggtcgtgtca gtcgaaccgg taattccagc cgatccattg gcagaggccg cggcccatga
      721 cgtcctcgag gtccccgccc ccgagccacg gcagttcctc ggtgaagaag gtcacgcatt
      781 ggcggtagct gcattgcatc cgcgtgatgt cggtccccca gaagaagcgc ttgggtccga
      841 acgcatcgaa gatgcggtgc agcccgtcct ggatgttgcg gaacggatag ctctgcgtcg
      901 aataatgcgg cgcgccggtc gctttcacgg cgacattggg caatcccgcc agcgccacga
      961 gctcgtcgag atggatgaaa gcctccgcat cgcggccgcg ccggttgagc ccgcagtgat
     1021 cgacgatcat cctcaaacgc gggtggcgct cggcaatctc gcggaacttt ttcaggaaaa
     1081 gaccgcccat catcgcgatc ggcagcccct cattttcggc cgccggccac agccaatcca
     1141 gcgagcggtc gtagagccac ttctgctgct cttccaggag aaacggccag cgcaggccca
     1201 tcatgccggg ctggtttcgc cagccatgga tcagctttcg attctcgggg tcttgcagcg
     1261 gaaactggcc catcaccgcg aaacgatcgg gatagcgcct ggccgcatcg accgcgagcg
     1321 cgttcgcgtt ggggtcccag ctaccaggcg gatggatcag tgcggcgtcg accccggccg
     1381 cgtccatctc ggccaaaacc tcttcggcgg tgaatttctc gaccttgcgg tgcaggcctg
     1441 acgtcggcac caccgtctgc gaccagatat gcacctgcgc gtcgacgatc ttcacggcgt
     1501 ttcctccgct ttctgatggc gcaatcttgc atgagcatcg agggtccggc aacgcgcgga
     1561 tatgacttgc cggatatgcc ttgccgcgga aacagcatgc gcccaccgca atgcaggcgc
     1621 ccttctctcg cgagcgccgc attgctatcg tgcgctccaa aacagggaag aaaccatgac
     1681 agggcccgag gacgacgacg gtctcgccgg caaggtcgcg ctgatcagcg gcggcggtgc
     1741 ggcgggcgac ggcatcggca atggccgagc ggcggcgatc ctgctcgcgc gtgccggaac
     1801 aaaagtgatg gtagccgatc gcgagccggc gctcgccgag cgtaccgtcg agatgatcac
     1861 gcatgaggga ggcacggccg ctgcgatagc cggcgatgtc accctcgagg cggactgccg
     1921 ccgtctggtc gaggcgaccg tcgcacgttt cgggcggctc gacttcctcg acaacaatgt
     1981 cggcatcggc agccgcggca gtgtggtcga ggagaagccg gagacgtatc ggcgcgtcat
     2041 gcaggtcaac gtcgagagca tgttcctgct gtcgaaatat gccatccccg cgatg
//
LOCUS       DATLJT010001767         1719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1822473,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001767 DATLJT010000000
VERSION     DATLJT010001767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822473"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VM910_39975"
     CDS             <1..214
                     /locus_tag="VM910_39975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68705.1"
                     /translation="EFFAPERHAAIAAVAGPNIDLCFVEKLHGVLAMPAEAVRQPEEL
                     QKSGHSGFGTRLFRPSVWYSLCKRGE"
     gene            258..1235
                     /locus_tag="VM910_39980"
     CDS             258..1235
                     /locus_tag="VM910_39980"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68706.1"
                     /translation="MRRRQFIATLGAAAVWPFGARAQQHAIPVVGFLNPTVSRLYEFN
                     AAAFRQGLKDAGFVDGENVRIEYRWAEGDYGQLPALAAELVKLKVAVIGATGDVASAR
                     AAKDATSVIPVVFTIGADPVKVGLVDSYNRPGGNVTGISAITSQIGAKRIEILYQLVS
                     WSKVALLMNPDNHAAETERQDAEAAVQALGRRAVVLNVASKDDFNGAFDSLIREGADA
                     LFVATDPMLLSQRETIATFAARQKIPAIHFERQYALQGGLISYGTSISGMYRQAGVYC
                     GQILKGANPAEMPVMQPTKFELVINLKTARSLGIEISSNLLAIADEVIE"
     gene            complement(1318..>1719)
                     /locus_tag="VM910_39985"
     CDS             complement(1318..>1719)
                     /locus_tag="VM910_39985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HVI68707.1"
                     /translation="FVLPGRTQTTAIFAAFQTASGDTHPIHYDVEYCRARGMPNLLAH
                     GFQTLIHTAPGAGLFPFLVEDSLVGFLEQSSRFLKPVYADDTIYPALEVTELTAGRTT
                     GVVTLQSTVYNQRRELVLEGWQKFLIRKRPA"
BASE COUNT          327 a          519 c          553 g          320 t
ORIGIN      
        1 tgaatttttc gcgccggaac gccacgcagc cattgccgcc gtcgccggac cgaacataga
       61 cctttgcttc gtcgagaaac ttcatggggt cttagctatg ccagcggagg cggtgcgaca
      121 acccgaagaa ttgcaaaagt ccggccattc gggctttggt acgcgacttt ttcgcccttc
      181 ggtatggtac agtttgtgca aacgcgggga atgaggggat cgatgcgccg ccgcgcgcgt
      241 gccgacgagg tgatcgaatg aggcggcgtc agttcatcgc gacgctcggc gcggcggcgg
      301 tttggccgtt tggcgcacgc gcgcaacaac atgcgattcc cgtggtcggc tttctcaatc
      361 cgaccgtgtc gaggctgtac gagttcaacg cggctgcatt tcgccagggc ctgaaggacg
      421 cgggctttgt cgacggcgag aacgtccgca tcgaatatcg ttgggccgag ggcgactacg
      481 gccagcttcc cgcgcttgcg gccgaactgg tcaagctcaa ggtcgccgtg atcggtgcca
      541 ccggcgatgt ggcgtcagcc cgcgcagcca aggatgcgac ctccgtcatt cccgtcgtct
      601 tcacgatcgg agccgacccg gtcaaggtcg gccttgtcga cagttacaat cgacccggcg
      661 gcaatgtgac cggcatcagc gccatcacca gccagatcgg cgccaagcgg atcgagatcc
      721 tctatcaact ggtcagttgg tcgaaggtgg cgctgttgat gaatcccgac aatcacgccg
      781 ccgagaccga acggcaggat gccgaggctg cggtccaggc tctgggacgt cgcgctgtcg
      841 tcctgaacgt ggcgagcaag gacgatttca acggcgcatt cgacagcctg atcagagagg
      901 gtgccgacgc cttgttcgtc gccaccgatc ccatgctgct cagccaacgc gagacgattg
      961 caacatttgc ggcgcgccag aaaatcccgg cgatccactt cgagcggcaa tatgcgctgc
     1021 aaggcggcct catcagctac ggcaccagta tcagcggaat gtaccgtcag gcgggtgtct
     1081 attgcggcca gattctcaaa ggcgccaacc cggccgaaat gcctgtcatg cagccgacca
     1141 agttcgaact cgtgatcaat ttgaagaccg ccaggtcgtt ggggatcgag atttcgtcga
     1201 acctgctcgc aatcgccgac gaggtgatcg agtgaggcgg cgggagttct tggcggcgct
     1261 tgcgcgcaaa gccatccgtg ggtgcgcgca agacgccgga tcgcttcgtc gcgcgcttca
     1321 ggcaggccgc tttcgaatca agaatttctg ccatccctcc agcaccagct cgcggcgctg
     1381 gttgtacacc gtgctctgca atgtcacgac gcccgtggtg cggcctgccg tgagctcggt
     1441 cacttccagc gcgggataga tcgtgtcgtc ggcatagacg ggcttcaaga accggctcga
     1501 ctgttcgaga aagccgacga gggaatcctc gaccagaaac ggaaacaggc ccgcgcccgg
     1561 cgcggtgtgg atcagcgtct ggaagccgtg cgccagcagg ttcggcatgc cgcgggcgcg
     1621 gcaatattcg acatcgtagt ggatcggatg ggtgtcgccg cttgcggtct ggaaggccgc
     1681 gaagatcgcc gttgtctgcg tacggcccgg caggacaaa
//
LOCUS       DATLJT010001768         2997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_212762,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001768 DATLJT010000000
VERSION     DATLJT010001768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212762"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VM910_39990"
     CDS             complement(<1..460)
                     /locus_tag="VM910_39990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVI68708.1"
                     /translation="MATNATTHDDHAHDDHAHDHPTGWRRWVYSTNHKDIGTMYLFFA
                     IIAGIIGAAMSIAIRAELMYPGVQIFHETHIYNVFVTSHGLIMIFFMVMPAMIGGFGN
                     WFVPLMIGAPDMAFPRMNNISFWLLPASFALLLISTFVEGEPGANGVGAGW"
     gene            complement(531..1391)
                     /gene="coxB"
                     /locus_tag="VM910_39995"
     CDS             complement(531..1391)
                     /gene="coxB"
                     /locus_tag="VM910_39995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVI68709.1"
                     /translation="MKMSKGRIGRHLLGSAVICGALAASGTAFAELGQPAPWEYTLQG
                     SATPVMDNIIWFHRVLLIIITVITLFVLALLVTVMVKFNSRSNPTPSRTTHNTLIEVA
                     WTLIPVLILVGIAVPSFRLLFLQLDLPKADLTIKATGKQWYWSYAYPDNGKFEFDSLL
                     ACDEERTKCDAPRLLAVDNEIVVPVDKVVRVETIGADVIHSFAVPAFGIKIDAIPGRL
                     NETWFKATKTGKFYGQCSELCGKDHAYMPIVVHVVTDQEFATWVEAAKKKFAAAKDTT
                     YASAAESAAR"
     gene            1884..2420
                     /locus_tag="VM910_40000"
     CDS             1884..2420
                     /locus_tag="VM910_40000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HVI68710.1"
                     /translation="MGPSKYLIKPVGGRAGLLAGAIATILLLGFAAAAHAQGAVRSVH
                     GDWQIRCDTPPGAQSEQCALIQSVVAEDRSNAGLTVIVLKTADQKSRLMRVVAPLGVL
                     LPSGLGLKLDNQDVGRAGFVRCLPNGCVAEVIMDDKLLGQLKAAKTATFIIFETPEEG
                     IGFPLSLNGLAEGFDKLP"
     gene            complement(2667..>2997)
                     /locus_tag="VM910_40005"
     CDS             complement(2667..>2997)
                     /locus_tag="VM910_40005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68711.1"
                     /translation="HRLIRQVALGYCSTELWEVIGWASIEMMASVYPIGVIIQKPEVW
                     PRARRLDAIASALVGHESALPVGAAASNFRIVVAVSKVQVLDQLLCTVGRSWKTTLKR
                     DDISNSH"
BASE COUNT          596 a          936 c          893 g          572 t
ORIGIN      
        1 tccagcccgc accgacgccg ttggcgccgg gctcgccctc gacgaaggtc gagatcagaa
       61 gcagcgcgaa ggaggccggc aacagccaga acgagatgtt gttcatacgc gggaacgcca
      121 tgtcgggcgc gccgatcatc aacggcacga accagttgcc gaagccgccg atcatcgccg
      181 gcatcaccat gaagaagatc atgatcagac cgtgcgaggt cacgaacacg ttgtagatgt
      241 gcgtctcgtg gaagatctgc acgccgggat acatcagctc ggcgcggatc gcgatcgaca
      301 tggcggcgcc aatgatgccc gcgatgatcg cgaagaacag gtacatcgtg ccgatgtcct
      361 tgtggttcgt cgaatagacc cagcgccgcc aaccggttgg atggtcgtgg gcgtggtcgt
      421 catgcgcgtg atcgtcgtgc gtcgtagcgt tcgttgccat ggtctaatcc tgccttgcag
      481 tccctttggg acctcaaccg gtcctcgccc ttgtccctcg cgcggcgccc tcagcgcgcc
      541 gcgctctccg cggccgacgc gtaggtcgta tccttcgccg ccgcaaattt cttcttcgcg
      601 gcctcgaccc aggtcgcgaa ctcctgatcg gtcaccacat gcacgacgat cggcatgtag
      661 gcgtgatcct tgccgcatag ctccgagcac tgcccgtaaa acttgccggt cttggtcgcc
      721 ttgaaccagg tctcgttgag gcggccgggg atcgcgtcga tcttgatgcc gaaggccggc
      781 actgcgaagg aatggatcac gtcggcgccg atggtctcga cacggaccac cttgtccacg
      841 ggcaccacga tctcgttgtc gaccgcaagc agccgcggcg catcgcattt ggtgcgttct
      901 tcgtcgcacg ccagcagcga atcgaactcg aacttgccgt tatccgggta tgcgtaagac
      961 caataccact gcttgccggt cgccttgatg gtgaggtccg ccttcggcag gtcgagttgc
     1021 aggaacagga ggcggaacga cggcaccgcg atcccgacca gaatcagaac cgggatcaag
     1081 gtccaggcca cctcgatgag ggtgttgtgg gtggtgcgcg acggcgtcgg gttggagcgg
     1141 gagttgaact tgaccatcac ggtcacgagc agcgccagca cgaacagggt gatgacggta
     1201 atgatgatca ggagcaccct gtggaaccag atgatgttgt ccatcaccgg cgtagcggat
     1261 ccctgcagcg tgtattccca cggcgccggc tgccctagtt cggcaaaggc cgtcccgctt
     1321 gccgccagcg ccccacaaat caccgccgac cccagcaggt gacggccaat tcggcctttc
     1381 gacatcttca tgccgttcgt gctccctaaa tcgaaaatca tccccacgac gcccgctgat
     1441 gcagcgagac gccatcgatc cgcccccgcc aaacggcctg aagcgcacct tcaagggggt
     1501 gccgggccag atcgtgagac acgccgaaat ccagcctctc aaaccataat tcttaagcca
     1561 tcgcaatgca ctcttggggg gcaggcggcg gcttctcccg cgtcgacgcc gccgtaacat
     1621 tttcccgtga gggaacgtcc aaacgcacgt ttggacgcat tagggcgctt gacaggtgtt
     1681 tacccccatg taggcacagg tacgacgatc ccgaccgcgc tgattcgtgg caaaaaacga
     1741 gaggacgcgg cgatcccggc gcaggttttg cttggaaaga ccgccttcaa ggcgccgtca
     1801 ttgccgtact cagtcggttg cgcgaagttc aaggaccgtc cgagcgactg gtcatacaag
     1861 ggacttacaa ggatccgacc ccgatggggc cttcgaaata cctgatcaaa cccgtcggcg
     1921 gccgtgccgg cttactggcc ggcgcgatcg ccacaattct gctcttgggc tttgcggccg
     1981 cggcgcatgc gcagggggcc gtgcgttcgg tgcatggcga ttggcagatc cgctgcgaca
     2041 ccccaccggg cgcgcagagc gagcaatgcg ccctgatcca gagcgtggtg gccgaggacc
     2101 gctccaacgc cggcctgacc gtgatcgtgc tgaaaaccgc cgaccagaag agccgcctga
     2161 tgcgcgtggt cgccccgctc ggcgtgctct tgccctccgg ccttggcttg aaactggaca
     2221 atcaggacgt cggccgcgcc ggttttgtcc gctgcctgcc caacggctgt gtcgccgaag
     2281 tgatcatgga cgacaagctg ctcgggcaat tgaaggccgc gaagaccgcg acgttcatca
     2341 tcttcgagac gccggaagag gggatagggt ttccgttgtc gctgaacggg ctggcggagg
     2401 ggtttgataa gctaccgtga ggggagacta gtggttttgg tcagccaaac ttcctgcgca
     2461 gctcttcggc ctcttcaggc aacttcaacc agtccaatcc tcgggcagca tttgttgcaa
     2521 gctgccgggt agtaggatga tcacgcccaa gcttgccatc gactatgccg agcgcgagtt
     2581 ctaagtacga tcgagctcca gcgtaatccc cctccctctg cagcagaatg ccgaaatgtc
     2641 gtcaccgagt tctctttcat ttgtcgtcag tgcgagttcg aaatatcgtc tcgcttcagt
     2701 gtagtcttcc aggaccgacc gacggtacaa agcagttgat ccaaaacctg cacctttgac
     2761 actgcaacga cgatgcgaaa attcgacgca gcagcgccaa caggcagagc actttcgtgg
     2821 ccgacaagcg ctgatgcgat tgcatcgagt cgtcgtgcgc gaggccatac ctcgggcttc
     2881 tgaataatta ccccaatcgg gtaaacgctg gccatcatct cgatagacgc ccacccaata
     2941 acttcccaga gctctgtgct acagtagccg agcgcgactt ggcgtatcaa tcgatgc
//
LOCUS       DATLJT010001769         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_674384,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001769 DATLJT010000000
VERSION     DATLJT010001769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674384"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VM910_40010"
     CDS             <1..701
                     /locus_tag="VM910_40010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68712.1"
                     /translation="VLDNEGKPIIVTVNRPTKALDLINQRPLIEGEIERRIRPEAAKS
                     CVSIREVRLGEPAIPPELLVAVRREQLATQLAKAFIQEKAAQEKRIDSENAKETANQQ
                     VTLVKAEIGVKASERNALAAKNEGQGERDKLALIAEGQKKQMDVLGVDSTVRLRQFEL
                     MLGTLTGFFEKHPEVLTAALTNAQKFVPNISVGGTEGGMSGLISAILGQSLATATAPV
                     HPTGQSDRTAAGQQ"
     gene            complement(768..1925)
                     /locus_tag="VM910_40015"
     CDS             complement(768..1925)
                     /locus_tag="VM910_40015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68713.1"
                     /translation="MKPVDAAITRMTEDGLAVVLGGRTYGDDTKADRIGNLRQMSVPK
                     EMDTFTEALARQSKLLALPAGSAQDAIYGAYGNDLELLTRYRNAFKRTIDAALRARAT
                     PDQPDAQAFLDRVTQEVAEIVHGPTLDLGPDTLRLEGNELLGRLQTQVRADVASLTQA
                     VAVWLHLLAEAEYVSIIEWFNPKALRYHFFRMEASRTELGRNKRTTGDMISGRTITTT
                     VQNRVEVFNERRVHTVVNARVQTPEEYRGRVPKRIALLLDAIPAEVRPFLTIIDGMVS
                     QEVIHRRVASSRIETQTHAVYIPDPALALFNSWAINGWGGSTPEPARSFYQGHAISNA
                     NKILALELIGTAAVTMLAAVAEGRRGAIAAGIICLILTLFQQLGMRIERKT"
BASE COUNT          449 a          688 c          702 g          440 t
ORIGIN      
        1 ccgttctcga caacgagggc aagccgatca tcgttacggt caaccggccc acaaaggcgc
       61 tcgacctcat caaccagcgt ccgctgatcg agggtgagat cgaacgccgc atccgtccgg
      121 aagctgcaaa atcgtgcgtg agcattcgcg aagttcgtct cggcgagcct gcgatcccac
      181 ccgagctcct cgtcgcggtt cgtcgcgaac agctcgcgac ccagttggcg aaggcattca
      241 ttcaggagaa ggccgcccag gaaaagcgca tcgactcgga aaacgccaag gaaaccgcca
      301 accagcaggt cacgctcgtc aaggccgaaa tcggcgtgaa ggcatccgag aggaatgcgc
      361 tcgcggcgaa gaacgaaggc cagggcgagc gggacaagct cgccctgatc gccgagggcc
      421 agaagaagca gatggacgtg ctgggcgtcg attcaacggt ccgcctgcgg cagttcgagc
      481 tcatgctagg cactctgacg ggcttcttcg agaagcaccc ggaggttctc accgcggcgc
      541 tgaccaacgc gcagaagttc gtgccgaaca tttcggtggg tgggaccgaa ggcggaatga
      601 gcggcttgat ttcggccatt ctcgggcaat cgctcgcgac cgcgaccgca cccgttcatc
      661 cgaccggtca atccgatcgg accgccgcgg ggcagcagtg atctgcttgg atgcgcgggt
      721 cgagcccgcg catgacgatc gagacgttgg agcgccttca tctccgatca cgtcttacgc
      781 tcgatgcgca tcccaagctg ctggaacaag gtcaggatga gacagatgat gccggcggcg
      841 atcgcaccac ggcggccttc ggcgacggcc gccagcatgg tgacggcggc agtgccgatg
      901 agctccaagg cgaggatctt gttcgcattc gatatcgcat gcccctggta gaacgagcgc
      961 gccggctccg gtgtcgatcc tccccagccg ttgatcgccc agctgttgaa cagggcgagc
     1021 gccggatccg gaatgtagac cgcgtgcgtc tgcgtctcga tcctgctcga ggcgacacgg
     1081 cgatgaatta cttcttgcga aaccatgccg tcgatgatcg tcaagaacgg ccgcacttcc
     1141 gccgggatgg cgtcgaggag aagcgcgatc cgcttcggca cgcgcccacg atattcctcg
     1201 ggtgtctgga cccgggcgtt cacgacggtg tgtacccgcc gctcgttgaa cacctcgacg
     1261 cggttttgga cggtcgtggt gatggtgcgg ccgctgatca tatcgccggt cgtcctcttg
     1321 ttgcgtccga gttcggtccg agacgcctcc atgcggaaaa aatgataacg caacgccttc
     1381 gggttgaacc actcgatgat gctcacgtat tcggcttcgg ccagcaggtg cagccagacg
     1441 gcgacggcct gcgtgaggga agcgacgtcg gcgcggacct gagtctggag tcgccccagc
     1501 agttcattgc cttcgagccg cagcgtgtcc ggcccgaggt cgagcgtcgg cccatgcacg
     1561 atctccgcga cctcttgagt cacgcgatcg agaaaagcct gggcgtctgg ctggtcggga
     1621 gttgcccgcg cccgcagcgc cgcatcgatc gtccgcttga acgcattgcg gtagcgggtc
     1681 agcagctcga gatcattgcc atacgcgcca tagattgcat cctgcgcgct gccggccggt
     1741 aaggcgagca gcttcgattg ccgcgccaat gcctcggtga aggtgtccat ctcctttggc
     1801 acgctcatct gcctgaggtt gccgatgcgg tcggccttgg tgtcgtctcc gtaggtgcga
     1861 ccgccgagaa ccacagcaag cccatcctcg gtcatgcggg tgatcgcagc atcaaccggt
     1921 ttcatggtcg tctctctctg tcgaagctcc gcgacggagc tgttagtcca cattaaatca
     1981 tatccattgg aactcgtcgg atcaaaaaca gagacgaaat attattgatt aacatcagat
     2041 aattatctgg cgccactgac gcaacgccgc ggtgcctgtc ccttccgaaa atgggatatg
     2101 cgtgtcagct ctttcttctc ctcataatgc taattgagag ccggtaattg tgatggccac
     2161 tggatcgctg cagggaaacg tcaattcgag agcttggtcg agaagggcac taatccgatg
     2221 gtatttctca cgttccacgg cggtgcgcga agcttttgtg tttgagatgc gcatcataa
//
LOCUS       DATLJT010001770         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1824730,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001770 DATLJT010000000
VERSION     DATLJT010001770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824730"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1036)
                     /locus_tag="VM910_40020"
                     /pseudo
     CDS             complement(1..1036)
                     /locus_tag="VM910_40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971427.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1040..2053)
                     /locus_tag="VM910_40025"
     CDS             complement(1040..2053)
                     /locus_tag="VM910_40025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI68714.1"
                     /translation="MQYRQLGRSGLKISPICLGTMMFGGPTNEATASRIVAKAREAGV
                     NFIDTADNYNKGGSEQVVGRVISNNRPNWVLATKLANTMGDDPNRGGLSRRWVLQAAD
                     ESLKRLGTDYIDIYYLHKEDHATPLEETVRAMAELIRSGKVRYFGVSNYRAWRVAEIC
                     NICDRLGIDRPVASQPYYNAMNRMPEVEHIPACGYYGLGVVPYSPLARGVLTGKYGVD
                     GAPDKDTRAGRNDTRMMQTEWRPESLKLAQEIKRHAESKGITAGQFAVGWVLNSSFVS
                     SVIAGPRTEEQWDDYVRALDYKFTAEDEALVDRLVVTGHPSTPGFNDPAYPIEGRKPR
                     TGG"
     gene            complement(2159..2581)
                     /locus_tag="VM910_40030"
     CDS             complement(2159..2581)
                     /locus_tag="VM910_40030"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HVI68715.1"
                     /translation="MTDKAAAKLKSDGWSIVETTGFIHLIGPLWHRLVDGHHEYALIA
                     QDKHHNRRGLVQGGVLMTFADRTCGMTARHVTGKHTMATIQLDVHFVEAGKIGETLVS
                     RPRVVRATRSMIFVNTEVTVDRRCIAMASGVFKILKSE"
BASE COUNT          472 a          873 c          872 g          512 t
ORIGIN      
        1 aggcgctgct cgatcgtgcg caggcgctcg tcgagcacag cctgatgccc tgtcgccgcc
       61 gcaagcagcg ccatgtgcat ttcaacgaag ggcaggctgg tcttgggaaa atgcgcttgc
      121 gcatagacct ccgcatcgcc ccaaagattt gccggcacgg cgtagccgca agccatcaag
      181 cgccacaata gcgatgcgca gtccgacatc gcgttcagcg gcggggccgc gttgactcgt
      241 ggttgtagaa tgtcggaata aatcgcaagc gccttcgccg tatcgccgag ctcgagcgcg
      301 ccgagcgcct gatgccaata gatgtggccg taaaggatac cgctgcggtc gtaggtgccg
      361 atccattgcg tgatcaaggc gtcggcatcg tccactgcgc caccctcgaa cattgcgtgc
      421 aacagcgcat gcgccgcata ggcgttgttg cgccgctgat caaaggcgcg ctcggtcatc
      481 gcgcgaccct tcccgacctg gccattctcg gtcagcgacc agccgtaatt ggagagaaac
      541 caccagtctc gccataatgc cgcgcgtggc gctcgcacag atcctgtcgt gcgcgatcgt
      601 gatcggccat gcccgagaag gcgaacaggc cgaacgcgcc gagcggcagc gacagcacca
      661 ccgcatcgcg cggaaagttt tcgagatgtc cgagtgccga tgacagcgcc gccggaagat
      721 tgccttcaac cgcaagcgcg agcgtctcga cgtggcccct ctcgcgcatg gtaccccgat
      781 gggcgacttt ctcgcgcgcc gccgctgcgg ctttccgtgc cgcctcgccc tgctgataga
      841 acgagtggat gcgcgcccgc gcagcgtgcg gaagtccgaa gtcgggatcg gcgtcgatcg
      901 cgcgctcgaa ggcttcggcc gcgccaggcc aggccgagag catgagatcg acgccctcgc
      961 gataggctga ggcagccgaa gccgaatccg tcgaaagggg caggccgtaa cggtcctcgt
     1021 ccaccgcggc gcccatggct caaccgcccg tccgtggctt gcgtccctcg atcggatagg
     1081 cgggatcgtt gaagccgggc gtcgagggat ggccggtcac gaccaggcgg tcgaccagtg
     1141 cctcatcctc ggccgtgaac ttgtagtcga gcgcacggac atagtcgtcc cactgctcct
     1201 ccgtgcgcgg gcctgcgatg accgagctca cgaaagacga gttcaggacc cagcccaccg
     1261 cgaactgacc ggcggtgatg cccttgctct cggcatggcg tttgatttcc tgcgcgagct
     1321 tcaacgattc cggccgccac tccgtctgca tcatgcgcgt gtcgttgcgc cccgcacgtg
     1381 tgtccttgtc gggtgcgccg tccacgccgt acttgccggt caaaacgccg cgcgccagcg
     1441 gactgtaggg tacgacgccg agcccgtaat agccgcaggc cgggatgtgc tcgacttccg
     1501 gcatgcggtt catcgcgttg taataaggct ggcttgcgac cgggcgatcg atgccgagcc
     1561 ggtcgcaaat gttgcatatt tcggccacgc gccaggcgcg gtagttcgac acgccgaaat
     1621 agcgcacctt gccgctacgg atcagttcgg ccatggcgcg cacggtctct tccagcggcg
     1681 tcgcatggtc ttccttgtgc aggtaataga tgtcgatgta gtcggtgccg aggcgcttca
     1741 agctttcgtc cgccgcctgc agcacccagc gccgcgacaa cccgccgcgg ttggggtcgt
     1801 cgcccatcgt gttggcgagc tttgtggcaa gcacccagtt cggccggttg ttggagatga
     1861 cgcggccgac cacctgctcg gaaccgccct tgttgtaatt atccgcggtg tcgatgaagt
     1921 tgacgccggc ctcgcgcgcc ttggcgacga tccgcgacgc ggtcgcttca ttcgtggggc
     1981 cgccgaacat catggtgccc aggcagatcg gcgagatttt caggccgctg cggcctagct
     2041 ggcggtattg catgggggag ttcctcacat tgacgggcgg caggatagcg ccattgcgag
     2101 cgcaagcgaa gcatcagcgc cgcaaaacgc gatgtccgga ttgctccgac gatcgtgctt
     2161 actcgctctt gaggatcttg aacacgccgc tcgccattgc gatgcaacgc ctgtcgacgg
     2221 tcacttccgt gttcacgaag atcatgcttc gggtcgcgcg gaccacgcgg ggccgtgaga
     2281 ccagggtctc gccgatcttt ccggcctcga cgaaatgcac atcaagctgg atggtggcca
     2341 tggtgtgctt gccggtcacg tggcgtgcgg tcatgccgca ggttcgatcg gcaaaggtca
     2401 tcagcacgcc cccttgcacg aggccgcggc ggttgtggtg cttgtcctgg gcaatcagcg
     2461 catattcgtg atggccgtcg acgaggcgat gccacaaggg accgatcagg tggatgaacc
     2521 ccgtcgtctc gacaatgctc cagccgtcgg atttgagctt tgcggcggcc ttgtcggtca
     2581 tgccggatcg tccactcctg aatgcgccgt catttcatcg gctgcttgcc tggcctagtc
     2641 aagcgatgaa ggccgccgca ggcgatcggc tgacaccggc tttggcagaa aatgccggat
     2701 aaatgtcatt gctgccaaag cgcgaattg
//
LOCUS       DATLJT010001771         3819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4577084,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001771 DATLJT010000000
VERSION     DATLJT010001771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4577084"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VM910_40035"
     CDS             <1..428
                     /locus_tag="VM910_40035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68716.1"
                     /translation="KTLMRYMGYTGIATAHGFRSTFKDWATEQTDFRTEAIELALAHA
                     VGSKVEAAYRRGELLEKRRLLSKAWGDFCLGLTPPAASNLIPFPGSVPDDRATEPVPI
                     AASSPKKLRRSIPLTAKAASITARPSPKSKDRRQLELDI"
     gene            complement(925..2253)
                     /gene="repC"
                     /locus_tag="VM910_40040"
     CDS             complement(925..2253)
                     /gene="repC"
                     /locus_tag="VM910_40040"
                     /inference="COORDINATES: protein motif:HMM:NF040974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein RepC"
                     /protein_id="HVI68717.1"
                     /translation="MDQENCAQEAPRGSFTRAPTGFRRLTPGLLKADRTAEKFTGLPE
                     GVESPGQLLAALKAAASRLGIASRLVHAVDWLFCFTQPQDWQKDSRPIVWPSASMQAI
                     ALGLSPTQVKEINRRLIELGLVTMKDSPNGKRYGKRDPKGRIIEAYGFDLAPIAARYA
                     EFQRVAVEGRAERRAMSRLRRRATIARKAITQILETAQEYGFEGEEWQVFANETEALA
                     LALKAVERVDEMEMGVRSLERRQQAARERLELLLGTVETAPMGTENRPHQYNYKPSPN
                     PEEDTVTAANKCNRAGRSGVSRSSVPVQPARAEKGMVHGIRPDELVRLTPKLKPYLRR
                     PDTTWPDIIDAADWLRHDLGVSKSLWGDACLAMGRELAAVALAIVSTKEPEHFRTSPG
                     GYFHGMVAKAIAGELHLDGTVWALRQASEPERHERGRGGGSRRRDADWVS"
     gene            complement(2445..2651)
                     /locus_tag="VM910_40045"
     CDS             complement(2445..2651)
                     /locus_tag="VM910_40045"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HVI68718.1"
                     /translation="MQTTENAVIDKDRAYRRRLTAQGQHQLVVALPRETVALIDELKE
                     RHGLRNRSQALMQLIEQGREIAQQ"
     gene            complement(2957..3277)
                     /locus_tag="VM910_40050"
     CDS             complement(2957..3277)
                     /locus_tag="VM910_40050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68719.1"
                     /translation="MTSKKLGALLKSVPPATARAAPEIIPVSAVEKPAPAPRRAVLPA
                     AKKEPEVPLQVLVPAHIRQELGIMVAKERTSLRSLILRGIRGLGIEVTAEEIKGKRGR
                     RHSF"
     gene            complement(3396..>3819)
                     /locus_tag="VM910_40055"
     CDS             complement(3396..>3819)
                     /locus_tag="VM910_40055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68720.1"
                     /translation="TIAALVGADLAIIPLKPAPDDVDAAIATYNLIREINETPERSGH
                     PIRAAMILTMTMRGTVIARHVRNELEAAGYPLLQAEMPHRVSYPEASIQGLSPNVVEP
                     DGAAAHDIADIVHELLNLENHEIMKSSEQEIMDKAVGA"
BASE COUNT          687 a         1183 c         1149 g          800 t
ORIGIN      
        1 tgaaaacgct gatgcggtat atgggctata ccggtatcgc caccgcacac gggttccgct
       61 cgacgtttaa agactgggcg accgaacaga ccgacttcag gaccgaggcg atcgagctgg
      121 cattggcgca cgccgtcggc agcaaagtcg aggccgctta tcggcgcggc gagcttctcg
      181 aaaaacggcg gctgttgagc aaggcctggg gcgatttctg cctcggcctg acgccacctg
      241 cggcatcaaa cctgatcccg tttcctggat cggttcccga cgaccgtgcg accgagccgg
      301 tgccgatcgc tgcgagcagc ccaaaaaagc tccgccgcag cattcctctc acagctaagg
      361 cggcgtccat caccgcaaga ccctctccaa agtcaaagga tcgccgccag cttgagctgg
      421 atatctaagc cacagaggca gtcggcgcga cgaccgccct cgatccaggc accgatcgcg
      481 tcgcggtcct ggttcgcagt caatttcatc gcgatcaggc gaccgagcgc acctctcttg
      541 aggagatgct cgataaaacc tttagccgtg taagttttgc ccgaacggcg gtgccaacga
      601 tcgcgatagg atcgtcaagc gcatcaacgg gaagctcggc aggaagagaa tccccgggcc
      661 catcgccgcg gctgtggacg atgcctcact ttccggcgtc agtgagcgat gccgcgtaca
      721 agggctcgaa cgcccggcgc agccctcgcc ggcggcggtc gggtctttgt tcatgatcca
      781 ttcggaatgg ggcctcgata gcgaccggat aggtcttcgg ccggaggcct tggtcgaacc
      841 cccgaagcgc cggagtgaac cgtagcgagc ccgcacatcg aggactcggt ttccggggat
      901 ccacgaaaag catgatagaa agcatcacga aacccagtca gcgtctcggc ggcgcgagcc
      961 ccctcccctg ccccgctcgt ggcgttccgg ctccgaggcc tgccgcaagg cccataccgt
     1021 gccgtcgagg tggagctcgc cggcaattgc cttggcgacc ataccgtgga aatagccgcc
     1081 cggggaggtc cggaaatgct ccggctcctt ggtcgagacg atcgcgagcg ccacggcggc
     1141 tagctcgcgg cccatggcaa ggcaggcgtc accccagagg gattttgaga cgccaaggtc
     1201 gtgccgcagc cagtcggcgg cgtcgatgat gtccggccag gtcgtatccg ggcgccgcag
     1261 atagggcttg agcttcgggg tgagccgcac cagctcgtcc gggcggatgc cgtgcaccat
     1321 gcccttctct gcccttgctg gctgcaccgg cactgaggat cgagagacac cggaccgccc
     1381 tgccctgtta cacttgttgg ctgcggttac tgtgtcctct tcaggattag ggcttggttt
     1441 gtagttgtat tggtggggtc ggttttccgt ccccatgggg gcggtttcca cagttcccaa
     1501 taggagctcg aggcgctcgc gcgcagcttg ctgccggcgt tccaggctcc tgacccccat
     1561 ctccatctca tccacgcgct ccacggcctt cagcgcgagc gccagggcct ctgtctcatt
     1621 ggcgaacacc tgccactcct cgccctcaaa cccgtactcc tgggccgtct cgaggatttg
     1681 ggtgatggcc ttgcgcgcga tcgtggcgcg tcggcgtagg cggctcatgg cacgccgttc
     1741 cgccctgccc tctaccgcca cacgctgaaa ttccgcgtag cgtgcggcta ttggtgcgag
     1801 atcaaagccg taggcctcga tgatgcggcc cttgggatcg cgctttccgt agcgcttgcc
     1861 gttcgggctg tctttcatcg tgaccaggcc aagctcgatg agccgacggt tgatttcctt
     1921 gacctgcgtt ggcgaaagac cgagcgctat tgcctgcatc gaggccgagg gccatacgat
     1981 cggccggctg tctttttgcc agtcttgtgg ctgcgtaaaa cagaacagcc agtcgaccgc
     2041 gtgcacgaga cgcgatgcaa tgccgagccg tgatgccgcg gccttcagtg ccgcgagcag
     2101 ctgcccaggc gattccaccc cttcaggcag tccagtaaac ttctctgccg tgcggtcggc
     2161 cttcagaagc ccaggcgtga gccgcctaaa gccggttggt gcacgggtaa agctcccccg
     2221 tggcgcctct tgggcacaat tttcttgatc catcggatcc tcctctttgt ggttggatcg
     2281 cgacgggcac gccttccccg ttaacggaaa cggcaatttc cgtttgacaa gaacggcgtc
     2341 ttttggctag gttgggggct gcgtgtaccc caacggtcgc cttgaaggcc cgtcacgaag
     2401 ttttgaaagc ccgcccggca aggcgggctt ttcagtctcc aggtctactg ctgggcgatt
     2461 tccctccctt gctcgattag ctgcatcagc gcctggctgc gattacgcag accatggcgt
     2521 tccttgagct cgtcaatgag ggctactgtc tcgcgcggca gcgcgaccac gagctgatgc
     2581 tgtccctgcg cggtaagccg gcgcctatag gcgcggtcct tgtcgatgac ggcattctcg
     2641 gtcgtttgca taagccccct cctctgcgag cgcgtgagtc gctagcgagc tcaaggtaaa
     2701 accatagctt gtggctgtcc atcgccctcc ggccagttct ggtatgattc gtcgtaatcc
     2761 cttggttttt ctggattgtc tcggatgcgg aaagttcgtg ggtgagtcaa acttcctggt
     2821 gttcgggaag cgagaacccc gaatcactca gagtctcggg tcgcgcccaa agggcccatg
     2881 aattcaataa ttcatgagtt cattagttga ggattttatg agctcatgaa tccatgattt
     2941 acatgaattc ctgaattcaa aaggaatgac gacggccacg cttgcccttg atctcctctg
     3001 cggtcacctc gatgccgagg ccgcggatgc cgcgcaggat gagcgaccgc aggctcgtgc
     3061 gctccttggc gaccatgatg ccgagctcct gacggatatg ggcgggcacc agcacctgta
     3121 gcggcacctc cggctctttt tttgcggctg gcagtacagc ccggcgtggc gccggggccg
     3181 gtttttcgac cgcagaaacg gggatgatct ccggggccgc acgtgcggtc gccggcggca
     3241 ccgacttcag cagcgcgccg agtttcttgc tggtcatggg ttcactacct cattcatgaa
     3301 ttcatgaacc ggtgagtcca tgatttcaca gtttccgaaa ttcattcgtt catgaaccgc
     3361 cacagttcgc agcaaaacct cctgcagaag ccggatcatg cgcccacagc cttatccatg
     3421 atttcctgct ctgaggattt catgatttca tggttttcta aattcagcag ttcatgaacg
     3481 atgtctgcaa tgtcgtgcgc tgccgcgcca tccggctcga cgacatttgg gctaaggcct
     3541 tgaatactgg cctcgggata agacacgcga tgaggcattt cggcttggag gaggggatag
     3601 ccggccgcct cgagctcgtt gcgcacgtgc cgcgcgatga cagtaccacg catggtcatc
     3661 gtgaggatca tagcggcccg tattgggtgc ccgctgcgct ccggcgtctc gttgatctcg
     3721 cgaatcaaat tatacgtggc aatcgcagca tccacgtcat ccggagcagg cttgagggga
     3781 ataatcgcga gatcggcccc gacgagcgcc gcaatggtt
//
LOCUS       DATLJT010001772         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2972488,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001772 DATLJT010000000
VERSION     DATLJT010001772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2972488"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VM910_40060"
     CDS             complement(<1..327)
                     /locus_tag="VM910_40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVI68721.1"
                     /translation="MDTERASPRYSDIDVWAPLDIAEAMIEGQFTAVAAVRGARFALL
                     EAALAAAERLRSGGRLVYVGAGTSGRLAVQDGAELVPTFSWPQERLLLLIAGGRNALL
                     RSVEGAE"
     gene            414..1331
                     /locus_tag="VM910_40065"
     CDS             414..1331
                     /locus_tag="VM910_40065"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVI68722.1"
                     /translation="MNALADFLLLGIDGGGSRCRARLCNIAGATLSEGVAGAANIRLG
                     VEQSFASVLQATIQCMSGAGLSSRDFERVVACVALAGASEPSQLEAARRHEHPYRMAV
                     FVTDAQAACIGAHGGRDGGIIVIGTGSIGWAELNGRQYQVGGWGWPISDEGSGAWLGC
                     EALRRTLWAHDGRMAWSALLRSLVAKFRSDPHAIVHWMTGASPRDFATFAPDIVEHAL
                     ANDPVAVELLRLAGGHVDALAQRLIEFGVHRLSLVGGLAGSIEPWLADSTKHHLVAPR
                     GDAVAGALQLARNAAESLAPGRTAPERSR"
     gene            1328..2359
                     /locus_tag="VM910_40070"
     CDS             1328..2359
                     /locus_tag="VM910_40070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVI68723.1"
                     /translation="MSETRVNMEAEWQKSPDAVRRQPSALAAPLSELLRCLRREPPNF
                     VLTSARGSSAHSATFGKHLIERHLGIPVGAFAPNVATIYKQRLKLNGQLFLTVSQSGR
                     SDDLIESVAMAKAAGALTATIVNDTDSPLAKSSDIVLPMAAGPELSVAATKTFVTSLS
                     AWLHIVAGWAGLDELRAAIERLPDRLTAAMRLDWSPALNALCEATSLVSLGRGPTLAI
                     AREAALKLKETSNLHAEAFSGAEFLHGPIALVESSYPALLFVPTDHAAAGLRALAADL
                     RRKNASVFMTDHGEQQASTLPALAPDNPDTDAVCLIQSFYGLAIRVAQCRGINVDLPR
                     HLQKVTRTR"
BASE COUNT          428 a          808 c          822 g          457 t
ORIGIN      
        1 ctcggcgcct tccaccgacc gcagcaaggc gttcctgccg ccggctataa ggaggagcag
       61 ccgctcttgc ggccagctga aggtcggaac gagctccgcg ccgtcctgga cggccagccg
      121 gccagaggtc ccggcgccga catagacgag ccgaccgccg cttcgtagtc tttccgcggc
      181 agcaagcgcg gcctccaaaa gtgcgaaacg ggcgcctctc accgcggcga ctgcggtgaa
      241 ttggccctcg atcatcgcct cggcgatgtc caaaggcgcc caaacgtcga tatcagaata
      301 tcgcgggctc gcgcgctcgg tatccatgga tcatgctgac tgttgacacg caggagaacg
      361 ctttccgatc ctagctggtt ctcgcgcgtt agggggttga cggagcatcg gaaatgaacg
      421 ccctcgcgga cttccttttg cttggaatcg atggcggcgg cagccgctgt cgcgcccgcc
      481 tttgcaatat cgcgggcgcg acgctaagtg aaggggtcgc aggcgcggcc aatattcgcc
      541 ttggagtgga gcaaagcttt gcctcggtcc tccaggccac gattcaatgc atgagtgggg
      601 caggattgtc gtcacgcgac ttcgaaaggg ttgtggcctg cgtggcgttg gcgggagcca
      661 gcgaacccag ccagctcgag gctgcgcggc ggcatgagca tccctatcgc atggcggttt
      721 tcgtcaccga tgcccaggcg gcatgcatcg gcgcacatgg cggccgtgac ggcggcatca
      781 tcgtgatcgg cactggcagc attggctggg cggagctgaa tgggcggcag tatcaagtcg
      841 gcggctgggg ttggccgatc tcggatgagg gaagcggcgc ttggctcggc tgcgaggcgt
      901 tgcgccgcac gctgtgggcc catgatgggc gcatggcttg gtcggcgctg ctgcgttcgc
      961 tggttgcaaa gtttcggtcg gatccgcacg cgatcgtgca ttggatgacg ggcgcttcgc
     1021 caagggattt cgccaccttc gcgccggaca tcgtcgagca tgctttggcc aacgaccctg
     1081 tcgcggtcga actcctgcgg cttgccggcg ggcacgtcga tgcgctggcg caacggctca
     1141 tcgagttcgg ggtgcatcgc ctgtctctgg tcggcgggct tgcgggatcg atcgagccgt
     1201 ggctcgcgga ctccaccaag catcatcttg ttgcgcctcg cggcgacgcg gtcgccggcg
     1261 cgctgcaatt ggcccgcaat gcggcggaat ccttggctcc cggccgcact gctccggagc
     1321 gttcccggtg agcgagaccc gcgtcaacat ggaagcggaa tggcaaaaaa gccctgacgc
     1381 cgtgcggcgg cagccgagcg cgctcgcagc tccgctgtcc gaactcctgc gttgcctcag
     1441 gcgcgagccg ccgaactttg tcctgacaag cgcgcgcgga agctcggccc attcggcgac
     1501 gttcggcaag catctgatcg agcgccatct cggcatcccg gtaggcgctt ttgcgcccaa
     1561 tgtggccacc atctacaagc agcggctgaa gctcaacggt cagctcttcc tcacggtctc
     1621 acaatccgga agaagcgacg acctcatcga gtcggtcgcg atggccaagg ccgcaggcgc
     1681 actgaccgca accatagtca acgataccga cagcccgctg gcaaagtcgt ccgacatcgt
     1741 gctcccgatg gccgcagggc ccgagctcag cgtggcagca acaaagacct tcgtgaccag
     1801 cctctccgct tggcttcata tcgtcgccgg atgggccggc ttggatgaat tgcgggctgc
     1861 aatcgagcgt ctgcccgatc gtctcacggc tgccatgcgg ctcgactgga gcccagcatt
     1921 gaatgcgctg tgcgaggcga cgagcctcgt gagcctggga cgcggcccca cgctcgcgat
     1981 cgcgcgagag gcggccttga agttgaagga gacctcaaat cttcatgccg aagcgttcag
     2041 cggcgctgaa ttcctccacg gtccgatcgc cctcgtggaa tcgtcatacc ctgcactcct
     2101 gtttgtgccg acggaccacg ccgcagccgg gctgcgggcg ctcgctgcgg atcttcgccg
     2161 caagaatgcc agcgtattca tgacggatca cggggagcaa caggcgagta ctttacccgc
     2221 gctcgcgccg gataaccccg ataccgacgc ggtatgcctg atccagagct tctacgggct
     2281 ggccattcgt gtcgcccaat gtcgtggcat caacgtggac cttccgcgcc atttgcaaaa
     2341 ggtaacgcgg accagatgaa gaggtcagag ctgcacgcca tcgcggcgga ccatgttttc
     2401 gacggcaccg tcgtgcacga gcgtaccgct gtggtcgtgg acggcgctcg catcgtggat
     2461 ctcgtcccga cgagcgatct gccgcggacc ttctccattc gcgagctgcc ggacg
//
LOCUS       DATLJT010001773         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2054718,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001773 DATLJT010000000
VERSION     DATLJT010001773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054718"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VM910_40075"
     CDS             complement(<1..941)
                     /locus_tag="VM910_40075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVI68724.1"
                     /translation="MQFTLNEDQLAVRDMAREFAAEKIAPHAVRWDEEKHFPVDVMRE
                     AAKLGMGGVYIRDDVGGSAMSRFDAALIFEALAQGCPTVSAFISIHNMALWMIDAFGN
                     EAQRKKWLPKLCAMEFLASYCLTEPGSGSDAAALRTRAVRDGDHYVLNGQKQFISGAG
                     GGDLYVVMVRTGTGGPSGISTIVVEGDTPGLSLGVNERKMGWNAQPTRAVIFENARVP
                     AANLLGEEGIGFKIAMAGLDGGRINMAACSLGGAQSALDKALAYMKDRKAFGKRLDEF
                     QALQFRIADMATELEAARTFVWRAADALDRKDPDATML"
     gene            complement(961..>2298)
                     /locus_tag="VM910_40080"
     CDS             complement(961..>2298)
                     /locus_tag="VM910_40080"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HVI68725.1"
                     /translation="QPEWAATNPQRRARVMMKFLELAQRDYDKLAELLAREHGKTIPD
                     AKGDIQRGLEVVEFAIGVPHLMKGEYTEGAGPGIDIYSLRQPLGVVAGITPFNFPAMI
                     PMWKFAPAIACGNAFILKPSERDPGVPMKLAELMIEAGLPPGILNVVNGDKEAVDAIL
                     DDPDIKAIGFVGSSPIAQYIYERAAASGKRCQCFGGAKNHAIVMPDADMDQAVDALIG
                     AGYGSAGERCMAVSVAVPVGKTTADRLMERLVPRVESLKIGTSIDPSADYGPLVTREA
                     VQRVKNYVDVGLKEGATLAVDGRNFRMQGYENGFYMGGCLFDNVTKNMRIYKEEIFGP
                     VLSVVRAHDYNEALALPSEHDYGNGVAIFTRDGDAARDFAARVNVGMVGINVPIPVPI
                     AYYTFGGWKKSGFGDLNQHGPDAIRFYTKTKTVTSRWPSGVKEGAEFSIPTMN"
BASE COUNT          406 a          755 c          701 g          436 t
ORIGIN      
        1 agcatcgtgg catccggatc cttgcggtcg agcgcgtccg cggcgcgcca gacaaaggtg
       61 cgcgccgcct ccaactcggt cgccatgtcg gcgatgcgaa actgcagtgc ctggaactcg
      121 tcgagtcgct ttccgaaggc cttgcggtct ttcatatagg cgagcgcctt gtcgagcgcg
      181 ctctgcgcgc cgccaagcga acaggccgcc atattgatgc gcccgccgtc gaggccggcc
      241 atcgcgatct tgaagccgat gccttcctcg ccgagcagat tcgcggcagg tacgcgggca
      301 ttctcaaaga tcaccgcgcg cgtgggctgc gcgttccagc ccatcttgcg ctcgttgacg
      361 ccaagcgaga gcccgggcgt gtcgccttcg accacgatcg tcgaaatgcc ggaaggtccg
      421 ccggtgccgg tccgcaccat cacgacatag aggtcgccgc caccggcacc cgaaatgaac
      481 tgcttctgac cgttgagtac gtagtggtcg ccgtcacgta ccgcgcgcgt gcgcagcgcc
      541 gccgcatccg atcccgagcc tggctcggtc aggcagtagc ttgccaaaaa ctccatagcg
      601 cagagtttcg gcagccattt cttgcgctgg gcctcgttgc cgaaggcatc gatcatccat
      661 aacgccatat tgtggatgga gatgaacgcc gagacggtgg ggcaaccctg cgccagcgcc
      721 tcgaagatca gcgcggcgtc gaaccgcgac atggcggagc cgccgacgtc gtcgcggata
      781 tagaccccgc ccatgccgag tttcgccgcc tcccgcatca cgtcaacggg gaaatgcttc
      841 tcctcatccc agcgcacagc atgcggagcg attttttccg cggcaaactc ccgcgccata
      901 tcacgaaccg caagctggtc ctcgttcagc gtgaactgca tttttccccg actttgaagg
      961 tcagttcatc gtcgggatgg agaactcggc tccttccttg acacctgagg gccagcgcga
     1021 agtgaccgtc ttggtcttgg tatagaagcg gatcgcgtcc gggccgtgct ggttcaagtc
     1081 gccgaagccc gacttcttcc agccgccaaa cgtgtagtag gcgatcggaa ccgggatcgg
     1141 cacgttgatg ccgaccatgc ccacattgac gcgggctgca aagtcgcgcg ccgcatcgcc
     1201 gtccctggtg aagatggcga caccgttgcc gtagtcatgc tccgacggca gcgccagggc
     1261 ttcgttgtag tcgtgcgcgc gcacgacgct cagcaccggc ccaaaaatct cctccttgta
     1321 gatgcgcatg ttcttggtga cgttgtcgaa caggcaaccg cccatataga agccgttctc
     1381 gtagccctgc atcctgaaat tgcggccatc cacggcaagc gtggcgccct ccttgaggcc
     1441 gacgtcgacg tagttcttga cgcgctggac cgcctcgcgc gtcaccagcg ggccatagtc
     1501 tgcggaagga tcgatcgagg tgccgatctt caggctttcg actcgcggga ccagcctttc
     1561 catgagccgg tcggcggtgg tcttgccgac gggaaccgcg accgagacag ccatgcagcg
     1621 ctcgccggcg gagccatagc ctgctccaat cagggcatcg accgcctggt ccatatcggc
     1681 atccggcatg acgatcgcgt ggttcttggc accgccgaaa cactggcagc gtttgccgct
     1741 cgcggccgcg cgttcataaa tgtattgcgc gatcggtgag gagccgacaa agccgatcgc
     1801 cttgatatcg gggtcgtcga ggatggcatc gaccgcttcc ttgtcgccgt tcacgacatt
     1861 gagaatgccc ggcggcaatc cggcttcgat catcagttcg gcgagcttca tcggcacgcc
     1921 cggatcgcgt tccgatggct tgaggatgaa cgcgttgccg caggcgatcg ccggggcgaa
     1981 tttccacatc gggatcatcg ccgggaaatt gaacggcgtg atgccggcga cgactccaag
     2041 cggctgccgc agcgaataga tgtcgatgcc ggggcccgcc ccttccgtat actcgccctt
     2101 catcagatgc ggaacgccta tggcgaattc cacgacctca agcccgcgct ggatgtcgcc
     2161 cttggcgtcg gggatggtct tgccgtgctc gcgcgccaaa agctcggcaa gcttgtcgta
     2221 gtcgcgctgc gccagctcga ggaatttcat catcacgcgc gcgcgccgct gcggattggt
     2281 cgccgcccat tccggctg
//
LOCUS       DATLJT010001774         1712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1594846,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001774 DATLJT010000000
VERSION     DATLJT010001774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594846"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..505
                     /locus_tag="VM910_40085"
     CDS             257..505
                     /locus_tag="VM910_40085"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HVI68726.1"
                     /translation="MPRATTAISLSPSDPTSLGQPTHFERQVTQLIERIKTTPRQPGV
                     DDIRIPSERAFRSRERALREGLEIDRVVFDALVALRAR"
     gene            complement(646..1620)
                     /locus_tag="VM910_40090"
     CDS             complement(646..1620)
                     /locus_tag="VM910_40090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HVI68727.1"
                     /translation="MPDAIRFHKSGGPEVLVSEPVPTGKPGPGEARVRHTAVGVNFVD
                     IYIRTGLYPAALPSGLGTEAAGVVEDVGPGVDHIKAGDRVAYAGGPLGAYSEVRVMPA
                     DRLVALPQGISDQQAAAMMLKGLTVQYLIRQIHKVTNGDTILFHAAAGGVGLIACQWA
                     KSLGATVIGTVGSEEKAKIAKAHGCDYPVIYTREDFVARVKEITRDQKLPVVYDSVGK
                     DTFMKSLDCLKPKGMMVSFGQSSGSVGPVDLGIFGQKGSLFFTRPTLNTYAAARADLS
                     AMAKDLFGVVLSGAVKIEVKQTYPLKDAAQAHRDLESRKTVGSTVLTV"
BASE COUNT          295 a          534 c          554 g          329 t
ORIGIN      
        1 gcgattgcgg ttccttcgtc gcgcgggccg atcgtactcg atatgggcac ctcagcttac
       61 atgatgaccg aggtgatgtt acgcgaacgg cttggagagt tacttcccga aggcgtcgcg
      121 ctcggccccg cgggcgagcc ggccagagat ccaacggcgg cacgacgcgg cgccttgctg
      181 ccgttcggcg gctacaaagg tttcgctctt gcgctgatga tgcaggcgct gggactgctc
      241 gccggttctg gcttggatgc cgagagcgac tacggctatc tctttgtcac cttccgaccc
      301 gacctctttg ggccagccga cacatttcga gcgccaggtg acgcagctga tcgagcgcat
      361 caagacaacg ccacggcagc ccggcgtcga cgacatccgt attccgtccg agcgggcatt
      421 tcgctcccgc gagcgtgcac tccgcgaggg cttggagatc gaccgggtgg tattcgatgc
      481 gctcgtagca ctgcgcgcgc ggtaaagggc gccggagtgg cgtatcttcc gacctgtggt
      541 ttccaaaaat tccatccagt cggattcgcg cggccaggca gaatcagaag tcgtccaaga
      601 actcgggtag cagggatggc gctcagtgcc ggggggttgc ttcgctcaca ccgtgagcac
      661 agtcgagccc acggtcttgc gcgactccag gtctcgatgc gcctgcgcgg cgtctttcag
      721 cggataggtc tgtttgacct cgatcttgac tgcgcccgag agcaccacgc cgaagagatc
      781 ctttgccatc gccgagagat cggcacgggc ggcggcgtag gtgtttagtg tgggacgggt
      841 gaagaacagc gagcccttct gtccaaagat gccgagatcg actggaccga ccgaacccga
      901 agactggccg aagctcacca tcatgccctt gggcttgagg caatcgagcg atttcataaa
      961 agtgtccttg ccgacggagt catagaccac cggtagcttt tgatcgcggg tgatttcctt
     1021 gacacgcgcc acgaagtcct cgcgggtata gatcactggg tagtcgcacc cgtgcgcctt
     1081 ggcgatcttc gccttctcct cgctcccgac tgtgccgatg acggtggcgc ccaacgactt
     1141 cgcccattgg caggcgatca ggcctactcc gcccgccgcg gcgtggaaga ggatcgtgtc
     1201 gccattggtg accttgtgga tctgtcggat gaggtactgg acggtcaggc ctttgagcat
     1261 catcgccgcg gcctgctggt ccgagatacc ttgcggcagc gccaccagcc ggtcggccgg
     1321 catcacgcgc acctcgctgt aggcccctaa tgggccgcca gcatacgcga cacggtcgcc
     1381 cgccttgatg tggtcgacgc ccgggccgac gtcctcaacg acaccggcgg cctcggtgcc
     1441 aaggccgctg ggcagcgctg cgggatagag gcccgtgcgg atgtagatgt ccacgaagtt
     1501 cacaccgacg gccgtgtggc gcacgcgcgc ctcgccgggg cctggcttgc cagtcgggac
     1561 gggctccgac accagcactt caggtccgcc ggacttatgg aaacggatag cgtcgggcat
     1621 gtcgcggctc ctctcgggca taaacggtca tcaatgacgg ttggcgttat agactaccgt
     1681 gactttgcgg tcgacgagtt cggcggcaag cg
//
LOCUS       DATLJT010001775         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1828343,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001775 DATLJT010000000
VERSION     DATLJT010001775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828343"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VM910_40095"
     CDS             <1..914
                     /locus_tag="VM910_40095"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI68728.1"
                     /translation="NSVTNYRGLPAVSYHDMTDFDLQQEVCAKAGITGRIISNPFAAE
                     VMTAISRKPAIDVVKHVNDRTVAVVARAKSNWGLGTLNPLDASHIAEGERCLGALGYK
                     GLLITSSWHGQFIDGEDAFAFWEWAQARQVPIFIHPPRVPIGHEQQMDQYKLDELVGR
                     PFDTAMALARMILSGLFDCYPDVKIAVAHMGGGLLPVIGRLDFGWRLGCDGMPERAVI
                     RCKRRPSDYLARLYVDTMGFWAPHVRETVEVFGADRVMFGTDYGPVPIDPGEHVDIVN
                     GLVISAADKEKILWRNAARFFNLDDLA"
     gene            1072..2130
                     /locus_tag="VM910_40100"
     CDS             1072..2130
                     /locus_tag="VM910_40100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68729.1"
                     /translation="MAEYRRKLEEYTLARGKYEAEADAYWGSVADKRKLRRAKRRNNQ
                     DIALGDYVLTQPPVYSGPPKPVDPSAPPEEVPPRRYVPVVADFLRAAAKEFNFVPQQP
                     RSEMEYKRAYAKAASAAGLTREQAVRIYAFESGGDGAYDVQAGLEQPKPGAQAISTAL
                     GYNQLLATNSVELMAEKGDQFIKILSAKAAGLTDEAKKTLQRKLVVLKSMVAFCRSVP
                     DTWSAHDKLANTPKGLGVHALNLDLDVGPLLQTQKLLDSVVFARAQGYGAVLSAAELE
                     MMNLTGDGNGLDIVMMPLASRERVPTSNFFQPGGYERNPIAGRSGVVSKLIAATNAVM
                     DRESNLQGAKDLAAVFPK"
     gene            complement(2569..2850)
                     /locus_tag="VM910_40105"
     CDS             complement(2569..2850)
                     /locus_tag="VM910_40105"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HVI68730.1"
                     /translation="MKAIWRGQVIAESDRTLEVHGYRYFPREAVRMDLLQLAPKTTSD
                     LACPHGVQFYDLAADAARSERAAWSYEAPHAAMKQIDHWIGFWNDVEIA"
BASE COUNT          547 a          889 c          931 g          531 t
ORIGIN      
        1 aaaattcagt gacgaattat cgcggtttgc cggcggtgtc ctaccacgac atgacggact
       61 tcgacctcca gcaggaggtt tgcgctaagg ccggcatcac cggccggatc atcagcaatc
      121 cgttcgcggc ggaggtgatg accgcgatct cgcgcaagcc cgcgatcgac gtcgtcaagc
      181 acgtcaacga ccggaccgtc gcggtggtgg cgcgggcgaa gagcaactgg ggcctcggca
      241 cgctcaatcc gctcgatgca agccacatcg ccgagggcga gcgctgtctt ggcgcgctcg
      301 gctacaaggg gctgctgatc acgtcgagct ggcacggcca attcatcgac ggcgaagacg
      361 ccttcgcgtt ctgggaatgg gcgcaagcgc ggcaagtgcc gatcttcatc catccgccgc
      421 gcgtgcccat cggacacgag cagcagatgg atcaatacaa gctcgatgaa ctggtggggc
      481 ggccgttcga cactgcgatg gcgctcgcac gcatgatcct gtcggggctg ttcgactgct
      541 atccggacgt gaagatcgcg gttgcacata tgggtggcgg gcttctgccg gtcatcggac
      601 ggctcgattt tggttggcgg ctcggctgcg acggcatgcc cgagcgcgcg gtcattcgct
      661 gcaagcgccg gccgagcgat tatctcgcca ggctttacgt cgacaccatg ggtttctggg
      721 cgccgcacgt gcgcgagacg gtggaggtgt tcggcgccga tcgcgtcatg ttcggcaccg
      781 attacggccc ggtgccgatc gatcccgggg agcacgtcga catcgtgaac ggactggtaa
      841 tctccgcagc cgataaagag aagatacttt ggcgtaacgc ggcgcgtttt ttcaacctgg
      901 acgacctcgc gtgaggtccg ctcggcaagg actgcagacg aaatgcggca ccgttcgctc
      961 catagatgct gcgggtgtgt gctcgcggcg ctgctgctct cgttaactca cggggcgaga
     1021 gccgaagctg ccgagcgccc ggcggcggcg agcgcaccgt cgccggccgc catggccgaa
     1081 tatcgtcgca aattggagga gtacaccctc gcgcgtggta aatacgaggc cgaggccgac
     1141 gcatattggg gttccgtcgc ggacaagcgg aagctgcgac gcgccaaacg ccggaacaat
     1201 caggacatcg cgctcggcga ttacgtattg acccagccgc cggtctattc gggtccgccc
     1261 aaaccggtcg atccctcggc acctcccgag gaggtgccgc cgagaaggta cgtgcccgtc
     1321 gtggccgact ttttgcgggc agcggcgaag gaattcaatt ttgttccgca gcagcctcgc
     1381 agcgagatgg agtacaaacg cgcctacgcc aaggccgctt ccgccgccgg gctgacgagg
     1441 gagcaggccg tgcgaattta tgccttcgag tccggcggcg acggcgcata cgacgtgcag
     1501 gcggggctcg aacagccgaa gccgggcgcg caggcgattt ccaccgcgct gggctacaac
     1561 cagctgttgg ccaccaacag cgtcgagctc atggcggaga agggcgatca gttcatcaaa
     1621 atcttgagcg cgaaggccgc gggactgacc gatgaggcaa agaagacgct gcagagaaaa
     1681 ctcgtggtgc tcaagagcat ggtcgctttc tgccgaagcg tgcccgatac ctggagcgcc
     1741 cacgacaagc tggcgaacac ccccaaaggt cttggcgtcc atgcgctgaa tctggatctg
     1801 gacgttggtc ctttgctgca gacgcagaag ctcttggact ccgtcgtgtt cgcgcgcgcg
     1861 cagggatatg gcgcggtgtt gagtgcggcc gagctcgaga tgatgaacct caccggcgat
     1921 ggcaatggtc tcgatatcgt catgatgccg ctggcatcgc gcgaacgagt gcccacctcg
     1981 aacttcttcc agccgggggg ctatgagcgc aatccgatcg ccggccgcag cggcgtcgtc
     2041 tcgaagttga tagctgcgac caacgcagtg atggatcgag agagcaactt gcaaggcgca
     2101 aaggacctgg cggccgtatt ccccaaatga atcatgacgg aaaggcttaa cgcgccggga
     2161 cctcggcggg gtcgtttgcg cctccacacg ctgtggtagg tattttcatt gtaaataaca
     2221 ggccggaaac aatggccggt cggtttctac aaccggcgct ttgcatcgcg atcctcccat
     2281 gacggatccc ccggcgcctg gccgcggccg cgatatgcgt ccgcgcagca aaaagcccgg
     2341 cggggtgtcc cgtccgggcg gctggcgtag cttcggtgaa tgcacttttc tggaagattc
     2401 tcgttacacg agtcaagagg gttatgggag gacttgtatc ctgcgctcgc tgcggcgcgc
     2461 caaagcttga atttacgtca gcttttcaag gcgctgcgga catagcccgg cgactagccc
     2521 cgaccacctt cgtcgcgatt gaccccaagc gcgcgggggt tgatccgctc acgcgatctc
     2581 aacgtcgttc cagaaaccga tccaatgatc gatctgcttc atcgcggcgt gcggcgcctc
     2641 gtacgaccat gcggcgcgct cgctgcgtgc cgcgtccgcc gcgaggtcgt agaactggac
     2701 tccgtgcggg caggccagat cgctcgttgt cttaggagcc agctgcagca aatccatccg
     2761 caccgcttcc cgcgggaaat accggtagcc gtgcacttcg agggttcgat cgctctccgc
     2821 aatcacctga ccgcgccata tcgccttcat ggatgaccat cctccgtccc ggctaaactg
     2881 gactcagtcg ttgcactt
//
LOCUS       DATLJT010001776         1597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3665808,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001776 DATLJT010000000
VERSION     DATLJT010001776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665808"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VM910_40110"
     CDS             <1..444
                     /locus_tag="VM910_40110"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491650.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVI68731.1"
                     /translation="LAYQKDMQEDKEGAMDAFSALLLSIAAMTGMVRDMTPDVARMRQ
                     AAGAGYSTATDLADWLVRTHNMPFREAHHVTGRIVAKAAAENVSLDRLPLAAMQAIEK
                     LITNDVFTVLSAEDSANSRTSYGGTAPKNVRRQANRWLKALEKER"
     gene            542..781
                     /locus_tag="VM910_40115"
     CDS             542..781
                     /locus_tag="VM910_40115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HVI68732.1"
                     /translation="MSSSSGLRFARIAAIGTLVAALGLAGCGRKAGLDPPPGATAADT
                     SPSRPDLEPAIGPDGKVIATPQGPKRSTFIDWLLN"
     gene            791..>1597
                     /locus_tag="VM910_40120"
     CDS             791..>1597
                     /locus_tag="VM910_40120"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVI68733.1"
                     /translation="MHHFAYRNGVLHAEAVNLVDLAQAVATPFYCYSTATLRRHYDVF
                     ADAFADVKALICYAMKANSNQAVIKTLAGFGAGADVVSEGELRRARAAGIAPDKIMFS
                     GIGKTAAELALAVDEGILCVNVESEGELELLSSIAKGKGRTARVSVRVNPDIDPKTHA
                     KIATGKSENKFGIPISRARDVYARAAKLPGLEVTGVDMHIGSQITELEPFDDAFALLS
                     DFVRTLRADGHAISHVDLGGGLGIPYREDNDPPPHPEAYAAMVKRATRDLD"
BASE COUNT          293 a          540 c          487 g          277 t
ORIGIN      
        1 ctcgcctacc agaaggacat gcaggaggac aaggaaggcg ccatggatgc tttctccgcc
       61 ctcttgttgt cgattgccgc catgaccggc atggtgcgcg acatgacccc cgatgtcgcg
      121 cgcatgcgcc aagccgctgg ggcgggctat tccaccgcga ccgacctcgc cgactggctg
      181 gtgcgtacgc acaacatgcc gttccgcgag gcgcatcatg tcaccgggcg aatcgtggcc
      241 aaggcggcgg cggagaatgt ctcgctcgac cgattgccgc tcgccgccat gcaggcgatc
      301 gagaagctca tcaccaatga cgtctttacg gtgcttagcg ccgaggattc ggcgaacagc
      361 cgcaccagtt acggcggcac cgccccgaaa aatgtgcgcc ggcaggcgaa ccggtggctt
      421 aaggccttgg aaaaggagcg ctgatagatt cctcgtttcg catgggaacg ggattgtttc
      481 gccacgctgt cgcctcttct gtccgatctg atatggtgct gccaattgag gagggcccga
      541 attgagttct tcatccggcc tgcgctttgc ccggatcgcg gctattggca cgcttgtcgc
      601 cgcgctcggc cttgccggtt gcggacgcaa agccggactc gacccgccgc cgggagcgac
      661 ggccgccgac accagccctt cgcggccgga cctcgagccg gcgatcgggc ccgacggcaa
      721 ggtcatcgcg acaccgcaag gacccaaacg cagcacgttc atcgattggc tgctcaactg
      781 agccagcgct atgcaccatt tcgcctatcg caacggcgtc cttcacgccg aggcggtcaa
      841 tctcgttgat ctcgcgcaag cggtcgcgac cccattctat tgctactcga ccgccacgct
      901 ccgccggcac tacgacgtgt tcgcggatgc ctttgccgac gtgaaggcgc tgatctgcta
      961 cgccatgaag gcgaattcca accaagcggt gatcaagacg ctcgccggtt tcggcgccgg
     1021 cgccgacgta gtgtcggaag gcgagctcag gcgcgcgcgc gccgccggca tcgcgccgga
     1081 caagatcatg ttctcgggca tcggcaagac ggcggccgaa ctcgcgcttg ccgtcgatga
     1141 aggcatcctt tgcgtcaacg tcgaatccga gggcgagctc gagctgctat catccatcgc
     1201 caagggtaag ggccgcacgg cccgtgtctc ggttcgcgtc aatcccgaca tcgaccccaa
     1261 aacccacgcc aagatcgcga ccggcaagtc cgaaaacaag ttcggaattc cgatcagccg
     1321 cgcgcgcgac gtttatgccc gcgcggcaaa actcccaggc ctcgaagtca ccggcgtgga
     1381 catgcatatc ggcagccaga tcacggagct cgagccgttc gacgacgctt ttgcgctgtt
     1441 gtccgatttc gtgcggacct tgcgcgccga cgggcacgcc atcagtcatg tggacctggg
     1501 tggcggcctc ggcattccct accgcgaaga caacgatccg ccgccccacc ccgaggccta
     1561 tgccgccatg gtcaagcgcg cgacgcggga tctcgac
//
LOCUS       DATLJT010001777         1659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_446839,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001777 DATLJT010000000
VERSION     DATLJT010001777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446839"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..>1659)
                     /locus_tag="VM910_40125"
     CDS             complement(232..>1659)
                     /locus_tag="VM910_40125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68734.1"
                     /translation="LFDKVWDGRIVSESTLTSRINAVRKAVKDTGKDQRLLRTVARKG
                     FRFVGDVRAEQPAAAPAQSASHPLKDGEPPRLALPALDRTAIAVLPFINMSGEAEQEY
                     FSDGISEDIITALSKLRWFYVIARNSSFVYKGKSVHLKQIGEELGAAYVVEGSVRKDG
                     ERVRITAQLNDVATGSHVWAERYDRSLADVFAVQDEITQAIVAAIEPQLYAAENFRAQ
                     RKAPDSMDAWDLVMRALSHYWRVTRQDNMVAQALLEKAIKIDPQYGQALGVLAASHSF
                     GAHMGWEEMAKAVPAAERAALAAIHADSEDAWAHYALASVYLFTRRFDDCLSEFELAL
                     KLNPSFSPARGYYGVALAYCGRWEEGDRAARQALRLSPRDPFAAIYYGVASYSQFVGG
                     NYEEAIGLAREGLRLRPDFVGAHRVLTAAAAMADHRELAAKALEALRRVQPNVSLSWL
                     ASEMPFKHDVEREHYSEAFRRAGLR"
BASE COUNT          329 a          513 c          491 g          326 t
ORIGIN      
        1 atcttccgga tgtgcgacgt atcggaataa cttgaataac tgctcaataa agtcatcgtc
       61 ttctactttc tctggtgctg cccctttttt ccaacatgca cctttggaag cgcgaagact
      121 ttcagagact catcattttt catccagaaa tgcttgaaat aatggccaga acggcgccgg
      181 tttcgccaca acgaagccgc ggggtcttct cactgcaccg agcggatagt gctagcgaag
      241 cccagcacgc cggaacgcct ccgagtagtg ctcgcgttcg acgtcgtgct tgaaaggcat
      301 ttcgctggcg agccaggaga gcgacacgtt cggttgcacg cggcggagcg cttccaacgc
      361 cttggccgcc agttcccgat gatcggccat ggcggccgca gcggtaagca cgcggtgggc
      421 accgacgaaa tcaggacgca gacgcagccc ctcgcgggcg agaccgatcg cctcctcgta
      481 gtttccgccg acgaactggc tgtaggaagc aactccatag tagatcgcgg caaagggatc
      541 ccgagggctc aggcgcaacg cctggcgcgc tgcgcgatcg ccttcttccc accggccgca
      601 ataggcgagc gcgacgccgt aatagcctcg ggcgggagag aaactgggat tgagcttcag
      661 cgcgagctca aattcggaga gacagtcgtc gaagcggcgc gtgaacagat aaacgctcgc
      721 cagcgcgtaa tgcgcccacg catcctcgct gtcggcgtga atggcagcga gtgcggcgcg
      781 ttcggcggcc ggtaccgctt tcgccatttc ttcccatccc atatgggcgc caaaggagtg
      841 gctggcggcc agcacgccga gtgcctgtcc gtattgcgga tctatcttga tcgccttctc
      901 gagcagcgcc tgggcgacca tattgtcctg ccgcgtcact cgccaataat gcgacagcgc
      961 gcgcatcacg agatcccacg cgtccatgct gtcaggcgct ttgcgctggg cccggaaatt
     1021 ctcggcagca tagagctgcg gctcgatcgc ggcgacgatg gcttgcgtga tctcatcttg
     1081 gacggcgaac acgtcggcca aactgcgatc gtagcgctct gcccagacat ggctgccggt
     1141 agcaacgtcg ttgagctgcg ccgtaatacg cacgcgctcg ccatccttcc ggacgctgcc
     1201 ctcgacgaca taggcggcgc cgagctcttc gccgatctgt ttcaggtgaa ccgacttgcc
     1261 cttgtacacg aaggaggagt tgcgggcgat cacatagaac cagcgtagct ttgaaagcgc
     1321 cgtgatgatg tcttcgctaa tgccgtcgga aaaatactcc tgctcggctt cgccgctcat
     1381 attgataaag ggcagaaccg cgattgcggt tcggtcgagt gccggaagag ccaatcgcgg
     1441 tggttcccca tcctttagcg gatgagacgc agactgcgcc ggcgccgcgg ccggttgttc
     1501 cgctcgcacg tcaccgacaa agcgaaagcc cttgcgcgca acggtgcgca ggagacgttg
     1561 atccttgcct gtgtccttta cagccttgcg caccgcattg atgcgactgg tcaacgtcga
     1621 ctccgagacg atacggccat cccacacttt gtcgaacag
//
LOCUS       DATLJT010001778         1844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2974694,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001778 DATLJT010000000
VERSION     DATLJT010001778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2974694"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VM910_40130"
     CDS             complement(<1..717)
                     /locus_tag="VM910_40130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68735.1"
                     /translation="MIDISLSRRRLLAGASATTALGLFAGTSATQAKAPVLNAPAPAF
                     YRFKLGTIEATVVSDGPLSIGDPKNTFRGPTAEELGKMMNDQFLPANNVVLDQNVLVI
                     NTGDKLALFETGMSSVKRNDDMGRLVANLKQSGIDPADVDAVIPTHAHIDHIGGIMAA
                     DGSRNFPNAQIYISQADLEFWTDDKRLGTPAEGSALAARKNLIPNRERIVFYKDGQEV
                     LPGVQAMHTPGHTVGHTSFVV"
     gene            969..1247
                     /locus_tag="VM910_40135"
     CDS             969..1247
                     /locus_tag="VM910_40135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68736.1"
                     /translation="MQRAHPPLSRVKKRKKTMPTTILICGSPADGFQIIGLFADRGDA
                     ESYAEDYSLKDWWIAELTSPEAFAEMQRQRLRALRGPRKIRKGPRIGV"
     gene            complement(1354..>1844)
                     /locus_tag="VM910_40140"
     CDS             complement(1354..>1844)
                     /locus_tag="VM910_40140"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HVI68737.1"
                     /translation="AYRRQVVDYHRKESPGSILVDTDARYVYYVLPDGKAVRYGAIVG
                     ERAWAGVATVGRKEEWPGWTPTEGEKRRLGPLPNYVEGGASNPMGARALYLYADGKDT
                     LYRIHGTNQPGWIGHAISSGCIRLTNEDVIDLYSRVKTGAVVVVLGPKQGASRVASAD
                     SR"
BASE COUNT          342 a          573 c          577 g          352 t
ORIGIN      
        1 taccacgaag ctggtatgtc cgaccgtgtg ccccggggta tgcattgcct gcacgccggg
       61 gagaacctcc tggccatcct tgtagaacac gatgcgctcc cggttcggga tgaggttctt
      121 gcgcgcggcg agcgcagagc cctcggccgg agtgccaagc cgcttgtcgt cggtccagaa
      181 ttccaggtcg gcctgggaaa tatagatctg cgcgttgggg aaattgcggc tgccgtccgc
      241 cgccatgatc ccaccgatat ggtcgatgtg cgcatgggtg gggatgaccg cgtcgacgtc
      301 ggcggggtcg atgccggact gcttgaggtt ggcgaccagc cgtcccatgt cgtcgttgcg
      361 cttgaccgag ctcatgccgg tctcgaacaa ggcgagcttg tcaccggtat tgatcacgag
      421 gacgttctgg tccagcacga cgttgttggc cggcaggaac tgatcgttca tcatcttgcc
      481 aagttcctcc gccgtgggac cgcggaaggt gttttttggg tcgccgatcg acagcggccc
      541 gtccgacacc acggtcgcct cgatggttcc gagcttgaag cgatagaatg ccggtgccgg
      601 cgcgttcagc accggcgcct tggcctgcgt cgccgacgtc ccagcaaata ggcccagtgc
      661 ggtcgtcgca gatgctccgg cgagcaagcg gcgccgcgac agcgaaatgt cgatcacgat
      721 ccgcctccct atgcgttttc ttgaggggcg gacggtcgtc cgcccgcgac gagtctactc
      781 caacgggagg ccgagttcac gatccttcca aatagaaatg atcccctgaa cgctggctcg
      841 cggtttcggc agatgatacg caatggtgct ttttctgagc ctgaaatcga ccatttcgtg
      901 atggccatca cagcggccga agccgaaatc tggcatagac cctaaccctg ccgcggaaat
      961 agccgccaat gcagcgcgcc catccgcctc tgagccgtgt aaaaaagaga aaaaagacca
     1021 tgcccacgac gatcttgata tgcggcagtc ctgcggatgg cttccaaatc atcggcttgt
     1081 tcgccgatag gggtgatgcc gaatcctacg ctgaagacta tagtctcaaa gactggtgga
     1141 ttgccgagct gacatcgccg gaagcgttcg cagaaatgca gcggcaacgc ttgcgagcac
     1201 tgcgcggtcc ccgaaagatt cggaagggac cccgtatcgg cgtgtgacgc aaatcgaggc
     1261 agctcgcgat ctgcgccgtc gcgggcaaag cgcaccgagc caagccatga gggtgtgtcc
     1321 gacttgcaag gggagacgtc caggatagcg cgtttatcgg ctgtcggccg atgcgacacg
     1381 cgaggctcct tgcttcggtc ccagcaccac cacaaccgca cccgtcttca cccggctgta
     1441 gagatcgatc acatcctcat tggtcaaccg gatgcagccc gacgaaatcg cgtgcccaat
     1501 ccatcccggc tggttcgtgc cgtggatgcg atagagcgtg tctttgccgt cggcgtagag
     1561 gtagagcgcg cgcgccccca tgggattgct tgcaccgcct tcgacgtagt tcggaagcgg
     1621 gccgaggcgg cgcttctcac cctcggtcgg cgtccatccc ggccactctt ccttgcggcc
     1681 gaccgtggcc acgccggccc aggccctttc gccgacgatc gcgccgtagc gaaccgcctt
     1741 gccgtcgggc agcacgtaat aaacgtagcg cgcgtcggta tcgaccagga tcgagccggg
     1801 cgattccttg cggtggtagt cgacgacctg acgccgatag gcgg
//
LOCUS       DATLJT010001779         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_447252,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001779 DATLJT010000000
VERSION     DATLJT010001779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447252"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VM910_40145"
     CDS             <1..1267
                     /locus_tag="VM910_40145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI68738.1"
                     /translation="ATDGRSQTGTNMLETYFSASKMLGHLRSGPSGPYLDGFAAALER
                     QGYSAGTAVRYLRAAAHLGHVAARQGAMPGDIDLAVFSEHLRTCRCPRAMGGRRNHHT
                     IFGARLFRQHLVEIGVCRSAAAALQHAEPCLVAHFKVWLRKHRGASDPSIKLYARDAA
                     HLMAALGDDPERWEPAAIRSFFLDRASHCGNGTVEKLTTSLRAFLRYLAVEGRCRAGL
                     ADVVPGYAHWRLADLPRYLAAEQVNRLIAACDGEVVERRRDRAILLLLARLGLRAGDV
                     AQLRLGDIEWETGSLRVSGKSRYEVRLPLPQDVGDAIAAYLSCRPSTCPSDHVFLRNI
                     APFRPFRKGDGVSSVVKRLMRQAGIVAPVKGAHVLRHTAATEMLRHGVPLEKIGLVLR
                     HRGIDTTAHYAKADVMLLKQIAQPWPEVL"
     gene            1267..2172
                     /locus_tag="VM910_40150"
     CDS             1267..2172
                     /locus_tag="VM910_40150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVI68739.1"
                     /translation="MLEAIEAYLALRRATGFAMLNAEYLLKSFAAFATERGQTHVHMQ
                     TAIDWAARGPSVAQRDARLKAVCRFVRHVRVEDARHELPPANHFGARKTRRSPHIYSA
                     TEIDRLIGAALRLRPKGGLRSRTYATLIALLSVTGLRISEALRLTVADVTRDGLLIRE
                     TKFRKTRLAPLHDTAVAGLKRYLARRRPRSKADPVFVDARGLPLRYIAVKETFDRLVG
                     SAGIVSAAKRRPRLHDLRHTFAVRALQDSPADRSRSGAHMAALATYMGHVNIYATYWY
                     LEATADLMRDVAAAGERFVAEGRQS"
     gene            2169..>2674
                     /locus_tag="VM910_40155"
     CDS             2169..>2674
                     /locus_tag="VM910_40155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HVI68740.1"
                     /translation="MTPIAPLIEAFLDETLARQRGASRHTCDSYALSFQLLFMFAAKR
                     LKISPSALTLEQLDAGLISAFLEYLEDQRKNASVTRNVRLAAIKSFFRFLEYRQPAAL
                     EHIRRVLAIPFKKTDRRLVPYLLREELQAVLDAPDPATRDGIRDRAMLHLAVCAGLRV
                     SELTGLKL"
BASE COUNT          441 a          878 c          863 g          492 t
ORIGIN      
        1 ggccaccgat ggccggtcac aaacggggac gaacatgttg gagacctact tctcggcatc
       61 gaagatgctg gggcatctgc ggagcgggcc gagcggacca taccttgacg gtttcgcagc
      121 cgcgctcgag cggcagggct atagcgccgg cacggccgtg cgatatttac gggcggcagc
      181 gcacctcggc catgtcgcgg ctcggcaagg ggcaatgccg ggcgacattg atctggcggt
      241 gttcagcgag cacttgcgca cttgccgatg tccgcgcgcc atgggagggc gccgcaacca
      301 ccacaccatt ttcggcgcta ggctctttcg ccagcacctt gtcgaaatcg gcgtttgccg
      361 gtccgccgct gccgcattgc agcatgccga gccgtgcctg gtggctcact tcaaggtatg
      421 gctgcgcaag caccgcgggg cgtccgatcc ctccatcaag ctctacgctc gcgacgccgc
      481 gcacttgatg gcggcgctcg gggacgatcc agaacggtgg gaaccggccg ctattcgcag
      541 ctttttcctg gaccgtgcga gccattgcgg caatggaacc gtcgagaagt tgacgaccag
      601 cctgcgcgcg ttcttgcgat atcttgctgt cgaaggccgc tgccgggcag gtctcgccga
      661 tgtcgttccc ggctatgccc attggcggct tgccgacttg ccgcgatatc tggcggcgga
      721 gcaggtgaac cggctgatcg cggcgtgcga tggcgaagtc gttgagcgcc ggcgtgatcg
      781 cgcgatcctc ctgctcttgg cgcgcctcgg gctgcgggcc ggcgacgtgg cgcaactccg
      841 cctcggcgac atcgagtggg agaccggctc gctgcgggtc tcgggcaagt cgcgctacga
      901 ggtgcgtctg ccgcttccgc aagacgtcgg ggatgcgatc gccgcctacc tctcctgccg
      961 cccttcgacc tgcccgagcg atcacgtgtt cctgcgcaat atcgcgccgt ttcgaccatt
     1021 ccggaaaggt gacggcgtct cgtccgtggt gaaacgcctt atgcggcagg ccggcatcgt
     1081 ggcgccagtc aagggagcgc acgtcctgcg acacacggcg gcgaccgaga tgctgcgcca
     1141 tggcgttccg ctcgagaaga tcgggttggt cctgcgccac cgaggcatcg acacgacggc
     1201 ccattacgcc aaggccgatg tcatgctcct gaagcaaatc gcgcaaccct ggccggaggt
     1261 tctctgatgt tggaggccat cgaggcttat ctggcgctcc gtcgtgccac tggattcgcg
     1321 atgctgaatg cggaatacct gctcaagagc tttgccgcct ttgcgaccga gcgcgggcaa
     1381 acgcatgtcc acatgcaaac cgcaatcgat tgggcggcgc gcggaccgtc tgtcgcgcaa
     1441 cgcgatgccc ggctgaaagc cgtctgccgc tttgtgcgtc atgtccgggt tgaagatgcc
     1501 cggcatgagc tgccgccagc caatcacttc ggcgcccgca agacgcggcg ctccccgcat
     1561 atctactcag caacggagat cgatcgcctg atcggggccg cccttcggct tcgacccaaa
     1621 ggcggcttgc gctcacggac ctatgcaaca ttgatcgcac tgctctcagt caccgggttg
     1681 aggatctccg aagcgctgcg gttgacggtc gccgacgtga cgcgcgacgg cctgctgatc
     1741 cgcgagacca agttccggaa gacccgcctg gcgccgctgc acgacacggc agtagcgggc
     1801 ttgaagcgct atctcgcgcg tcgccggccc cgttccaaag ctgatccggt gttcgttgac
     1861 gcgcgtggtc tgccgctgcg ctacattgcg gtcaaagaga ccttcgacag gctggtcggc
     1921 agcgcaggta tcgtatcggc ggcgaagcgc cgcccgcgtc tgcacgatct gcggcacacg
     1981 tttgcggtgc gtgcgctgca agacagtccg gccgatcgaa gccgaagcgg cgcgcatatg
     2041 gcggcgcttg cgacctacat gggtcacgtc aacatctatg cgacctactg gtacctggag
     2101 gccaccgccg acctcatgcg cgacgtcgcc gcggccggtg aaaggttcgt cgccgaaggg
     2161 aggcaatcat gacgccgatt gctcccctca tcgaagcgtt cctcgatgag acactcgccc
     2221 gccagcgggg cgccagccgg catacctgcg actcctatgc cctgagcttc cagttgctct
     2281 tcatgttcgc tgcaaaaaga ctgaagatct cgccgtcggc gctgacgctg gagcaactcg
     2341 acgccggcct catcagcgcc ttcctggagt atctcgagga tcagcgcaaa aacgcgtcgg
     2401 tgacgcgcaa tgtccggctg gcggccatca agtccttctt ccgcttcctc gaatacaggc
     2461 agccggcagc cctggagcat atccgccgcg tcttggcgat cccgttcaag aaaacggatc
     2521 ggcgtctggt cccctatctt ctgcgggagg agctgcaagc ggtgctcgac gctccggatc
     2581 ccgccacgcg cgatggcatc cgcgaccggg ccatgctgca tctggcggtt tgtgcgggtc
     2641 tgcgcgtgtc cgagctgacg gggttgaagc tcga
//
LOCUS       DATLJT010001780         4601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2286006,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001780 DATLJT010000000
VERSION     DATLJT010001780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286006"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VM910_40160"
     CDS             <1..415
                     /locus_tag="VM910_40160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HVI68741.1"
                     /translation="WIGNLMLLVLNLPLVGLWVKLLAVPYRLLFPAIFVFCCIGAYTV
                     DNKVFDVYEIAALSAVGYVLLKLDCEPAPMVLGYILGPIMEENLRRAMMISFGDPMVL
                     IMRPISAAFLFAAVALLVAIALPALRAKREEAMQE"
     gene            421..1095
                     /locus_tag="VM910_40165"
     CDS             421..1095
                     /locus_tag="VM910_40165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68742.1"
                     /translation="MVNRDRGDAEMIAKVVHCNGYSLAEDPRISRDKLECAPTEGRYS
                     DWEHVCWGDPNIGALGRIPMTLWLARHVDASLIIWSTGASRIIGGDYEATVFLNRALL
                     SFAELKRNFPDRFNDEAWSSERWYREWIQAISTTETTSQRTSDSLVEARKIVVERFDS
                     KPVILYSVSSANHAPRVLRDGLIAFEYGTGSRSTRRNMTLCAVAAETCYGAHTIRDVQ
                     VDDLGA"
     gene            complement(1165..1443)
                     /locus_tag="VM910_40170"
     CDS             complement(1165..1443)
                     /locus_tag="VM910_40170"
                     /inference="COORDINATES: protein motif:HMM:NF037270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68743.1"
                     /translation="MQIESDQTVCPACGAEGLEIFPVLHHMMCAYVGPQYDFAPAAAG
                     YICPKCRRDIVSGDLACEVVGTSARCVRCRKEMIVSPSPPPTFRANQN"
     gene            complement(1475..2530)
                     /locus_tag="VM910_40175"
     CDS             complement(1475..2530)
                     /locus_tag="VM910_40175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVI68744.1"
                     /translation="MDVKINRLDPNVPDPVESRRRAAGRLVRFAYATVVFGVLGFFVI
                     YFGAPFVYLSGPGTVTAPRHVVSLPYTVQVSQMKLVPGATVKAGEEIAEVISPEQDSI
                     VATYMRALADIAGRTAELRIKARVAQDLLEAARSYQLVTQEAVERLGVMTAATVTFRM
                     EVLREYASARKAVVSAEAEASESTVQLASLDEFIKQLRARLGEVESHFGKGRVLAPVA
                     GIVSTAIADVGQSLTAGTPIAEILDPTDIFVDWYIPNERLIDPTVGHEVFVLFGNRRI
                     PGTIAQILPVSGVYAGTQQQLLVRGRPATQIARIRFAPNASPPPLNTTVDVRMHYTAF
                     SAHVAGALFRLLGLYGN"
     gene            complement(2543..3796)
                     /locus_tag="VM910_40180"
     CDS             complement(2543..3796)
                     /locus_tag="VM910_40180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI68745.1"
                     /translation="MFEIVQLLAGTHLLLLAFSVLIFDIPRYTLSLMSLALFGVWKRS
                     DRVPAGNASVSVIIPTFNGGSGLAPTIASLHRQTLRPVEIIVVDDGSTDDTRAVAERA
                     RALGLVDIVICHGTRCGRSPAVNAAARFATGDLLLTVDADTVFEPTAVARLAAIFDDP
                     RVGGASCNIAISNERDSLWTGLQSVEYLMSISAGRSILDVVDAIACLSGACSMYRRDV
                     FVRQGGLDVGPGEDLEYSLRLRRLGYLIRFVPDAWAETDGPASGISLLRQRARWDRDA
                     LRVRFMMYGELSFFHRLERLPDTLQRLDFVIFDLVPTLSLPFYLAYIVLLFGTADAAL
                     FLAAVYVVLLWISIFNLALAFVMFNRSVGFFGLGLSLVFPLYQGVYLKCARFFSYSSE
                     IMFAASRYDDFVPPRVRRALLGTRI"
     gene            complement(4374..>4601)
                     /locus_tag="VM910_40185"
     CDS             complement(4374..>4601)
                     /locus_tag="VM910_40185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68746.1"
                     /translation="RPYYGGYYGGYYGYRIARRSSSIMLAGAKKHSAITLAGAKIRRV
                     NGVALLASSTAPSPRTHLPKRDASPQVLARR"
BASE COUNT          956 a         1350 c         1473 g          822 t
ORIGIN      
        1 gtggatcggc aacctcatgc tgctcgtgct caacctgccg ctggtcggct tgtgggtgaa
       61 gctgctggcg gtgccctacc ggctgctgtt tcccgcgatc ttcgtgttct gctgcatcgg
      121 cgcctatacc gtcgacaaca aagtcttcga cgtctacgag atcgccgcgc tctcggcggt
      181 cggctacgtg ctgctcaagc tcgactgcga gcccgcgccc atggtgctcg gatacattct
      241 cgggccgatc atggaggaga acctgcgccg cgcgatgatg atctcgttcg gcgacccgat
      301 ggtcttgatc atgcggccga tttccgccgc cttcctgttc gccgccgtcg cgctgctcgt
      361 cgccatcgca ctgccggcgc tgcgcgccaa gcgcgaggaa gcgatgcagg agtgatcagt
      421 atggtcaacc gggaccgggg cgatgccgaa atgatagcaa aagttgttca ctgcaacggg
      481 tattcgcttg ccgaagaccc gagaatctcg cgcgataaac ttgagtgcgc tcctacggaa
      541 gggaggtaca gtgattggga gcatgtatgc tggggtgatc caaatatcgg tgctcttggg
      601 cgaattccaa tgacgttgtg gcttgcccgc cacgtcgatg ccagcctcat catttggtcg
      661 accggagcga gccgaatcat cggtggcgac tatgaggcga cagtctttct caatcgtgcg
      721 ctgctgtcgt tcgcggagct aaaaaggaat tttcccgacc gtttcaacga tgaggcatgg
      781 tcgagtgaaa gatggtatag ggagtggatt caagccatat cgacaaccga aacaacgagt
      841 cagcgcacgt cagattcgct cgtcgaagct agaaaaatag ttgtcgagag gttcgattca
      901 aaaccggtaa ttctatattc ggtaagttcg gcaaatcacg cgccaagagt gttgcgagac
      961 ggactcattg ccttcgaata tggaacaggt tctcgatcga cccgcaggaa tatgaccctc
     1021 tgcgctgttg ctgcggaaac atgttacggt gcgcacacga tccgagatgt gcaagtcgac
     1081 gatctcggtg cgtaaggggg atgccgagcg aaatcatcgg gtcgctgacg cggcggtgcc
     1141 ggtcgaggct gaccgaagcc agcatcaatt ctggtttgcc cggaacgtcg gcggagggga
     1201 tggcgacacg atcatctcct tgcgacatcg gacacagcgc gcgcttgtcc ccacgacctc
     1261 gcacgcgagg tcgcccgaca cgatgtcgcg ccggcatttc ggacagatat atccagcggc
     1321 cgccggggcg aagtcatact gggggccgac ataggcgcac atcatgtgat gaagaaccgg
     1381 gaagatctcc aggccttcgg cgccgcaagc cgggcacacc gtctgatccg attcaatttg
     1441 catcgtggag cactccgcac ggtgtccgcg gccgtcagtt tccataaagc ccaagcagcc
     1501 ggaacagcgc gccggcgacg tgggcggaaa atgcggtgta gtgcatgcga acgtcgaccg
     1561 tcgtgttcag cggcggcggc gacgcgttcg gagcgaagcg gatgcgcgcg atctgcgtcg
     1621 caggacggcc acgcaccaac agctgctgct gcgtgccggc gtacacgccg gaaaccggca
     1681 ggatttgggc gatcgttccg ggaatgcgcc ggttgccgaa cagcacaaag acttcgtgtc
     1741 cgaccgtggg atcgatcagg cgctcattgg gaatgtacca gtccacgaag atgtcggttg
     1801 gatcgaggat ttcggcgatc ggcgtgcccg ccgtgagcga ctggccgaca tcggcgatag
     1861 ctgtcgaaac tattccggcg accggagcaa ggacgcggcc cttgccgaaa tggctctcta
     1921 cctcaccgag gcgtgctcga agttgtttga taaattcatc aagcgaggcg agttgaacag
     1981 tggactcgga ggcttccgcc tcggcggaga caacggcttt gcgggccgag gcgtattcgc
     2041 gcaaaacttc catgcggaag gttacggtgg ccgccgtcat cacgccgagg cgttcgactg
     2101 cttcctgggt cacgagctga taagagcgcg ccgcttcgag aaggtcctgc gcgacgcgag
     2161 ccttgatgcg cagttccgcc gtgcggccgg cgatgtccgc cagcgcgcgc atataggtcg
     2221 cgacgatgct gtcctgctcc ggtgaaatca cctcggcgat ctcttcgccg gccttcaccg
     2281 tggcgccggg caccagcttc atctggctga cctgcacggt ataagggagc gaaaccacat
     2341 gacgcggggc cgtgacggtg ccgggaccgc tgagatacac gaagggcgcg ccgaaataga
     2401 taacgaagaa accgagcacc ccgaacacga ccgttgcata ggcaaagcgc accagccgtc
     2461 cggcagcgcg ccggcggctt tcgacgggat cgggaacgtt gggatcgagg cggttgatct
     2521 tgacgtccat cgggccggct cctcaaatcc gagtccccaa gagggcgcga cgaacgcgcg
     2581 ggggtacgaa gtcatcgtac cgcgacgcgg cgaacatgat ttccgaggag taggaaaaga
     2641 accgggcgca tttcagatag acgccctggt acagcggaaa gacgagcgat agcccgagac
     2701 caaagaagcc gaccgatcga ttgaacatga cgaacgcaag cgccaggttg aaaatcgaaa
     2761 tccagagcag gacaacgtag acggctgcca gaaacagcgc ggcgtcggcg gtgccgaaca
     2821 acagtacgat gtaagccaga tagaacggca agctgagcgt gggaaccaga tcgaagataa
     2881 cgaagtccag ccgctgcaac gtgtccggca ggcgctcgag ccgatggaaa aagctcagtt
     2941 cgccatacat catgaaacgc acgcgcaacg catcgcgatc ccagcgggcg cgctggcgca
     3001 acaggctgat cccgcttgcc gggccgtctg tttccgccca ggcatccggc acgaagcgga
     3061 tgagatagcc gagccggcgc agccgcaagc tgtactccag atcctctccg gggccgacgt
     3121 ccagaccgcc ttgccgcaca aatacatcgc gccggtacat ggagcaggcg ccggacaggc
     3181 aggcgatcgc gtccacgacg tccagtatgg atcgcccggc gctgatcgac atcaggtatt
     3241 cgacgctttg tagcccggtc cacaacgagt cgcgctcgtt gctgatcgca atgttgcaac
     3301 tggcgccgcc gacgcgcgga tcgtcgaata tggcggcgag ccgcgcgacg gcggtcggct
     3361 cgaaaaccgt atccgcatcg acggtcaaga ggaggtcgcc ggtggcgaac cgcgccgcgg
     3421 cgtttacggc cggactgcgg ccgcagcgcg tgccatggca aataactatg tccaccaggc
     3481 ccagcgcgcg cgcccgctcc gcgaccgcgc gcgtgtcgtc ggtcgagccg tcatccacga
     3541 caatgatctc gaccggccgc aaggtttgcc ggtgcagcga ggcgatcgtg ggggcgagtc
     3601 cggagccgcc gttgaacgtc ggtatgatca cgctgaccga agcgttgccc gcaggcactc
     3661 tatcgcttcg tttccacacg ccgaacaaag ccaatgacat cagcgagagc gtgtagcggg
     3721 gaatgtcgaa tatcagtacc gagaaggcga gcagcaacaa atgggttccg gcgagcagct
     3781 gcactatttc gaacatgtga cgtgccctcg gaaggctaac aagacgctcg ccgaatgcgc
     3841 ccgggatcgg aaaagagtcg gcgcccctta gaagctcgat atcgtcacga cgccggtggg
     3901 cgctctaagc aatgaacttc ggcgtggctc ttgttgatcg acgaaagctc tttcgggcgg
     3961 gcgagcggct atccgtgcga tctccgaacc cctcggtgtg cgtgcggaga ttgtcgaatt
     4021 ttacaggacg taacgaaaac tggccattgg ctggggcggc ctcgggtggg gcgccttccg
     4081 ccgtgcgccg tatatgcccc atgccatcgt atattgattg ataataacag ttgaaccgct
     4141 gtcatgctgg cagcgcggcg ggcggtattt gggtcgggat ccttttcgta ccgtttcgcc
     4201 tatcagaccg agatgtagga cgccaacggc gagaactctt cgagcacatc gccggcgtcg
     4261 ttatcaggtc gcgatggcga tctagcgggc cgcggtcgag cgctggcgag gcggggccat
     4321 tacccttccc agggcgcacg ggtgatgacc cccaaaccgg acctagatgg cgttcagcgc
     4381 cgtgctagca cctgtggcga agcgtcccgc ttaggtagat gagtgcgagg cgaaggcgcc
     4441 gtcgaacttg caagcaacgc gacgccgttg acgcgacgaa tctttgcgcc ggccaaggtg
     4501 atcgcggaat gcttcttagc gccagcaagc atgatggatg acgatcgccg cgctatccgg
     4561 tagccatagt agccgccata gtacccgccg tagtaaggcc g
//
LOCUS       DATLJT010001781         5311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1830824,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001781 DATLJT010000000
VERSION     DATLJT010001781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830824"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1174)
                     /locus_tag="VM910_40190"
     CDS             complement(188..1174)
                     /locus_tag="VM910_40190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68747.1"
                     /translation="MRLTNVIARVLAACAGLLFATAALAQSPGYPDRPVKIIVPFAAA
                     GPTDAVARLIALKLSEKYGQQFYIENMAGAGGNLGMGAGARAPADGYTILFVSSSYTV
                     NPSLYAKPPYDPDKDFAPVTKAAGSPNGLFVHPSIPAKSVKELVELIKANPGKYTFAS
                     PGIGTTPHLSSELFKLTFGLDFALAPFPGGGPSIQSVVAGHTPMCFQAIPPATPLVKD
                     GKLRALAVTAATRSPALPDVPTLDELGIKNQEAETIQGVLLPAGTPKSIVDLLQTEIA
                     RIINLPDVKDKLLAIGLEPDGMSPAAFAAYIKADVGKWKKVIADAKIPQIGG"
     gene            complement(1446..1739)
                     /locus_tag="VM910_40195"
     CDS             complement(1446..1739)
                     /locus_tag="VM910_40195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HVI68748.1"
                     /translation="MPTFMCSLSWTEQGIRAIKDAPKRAKAAQDLAKKVGVEIKEVYL
                     TSGDSDLLVIIDTPNGDNAAKFALALGSKGSVRTRTARIWPQSEFHKLVSQLP"
     gene            complement(2006..2491)
                     /locus_tag="VM910_40200"
     CDS             complement(2006..2491)
                     /locus_tag="VM910_40200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68749.1"
                     /translation="MPKIAGFLALICVLALCGATASAQTCEYAGQTFSLGSTVCECPN
                     LRIVRSANSAGRGEITSRRLTCSKDQSWVNTNSLCLVAYTSADHAEDAFRKFQASYCP
                     RLPVNHAELLKAITEETEKFFSLAPRSQVVVAVQAICRRFANLSEHCQAMIEGLAASG
                     N"
     gene            complement(2671..4542)
                     /locus_tag="VM910_40205"
     CDS             complement(2671..4542)
                     /locus_tag="VM910_40205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVI68750.1"
                     /translation="MKALMRSRMSLFTACTAAFAGGLALLISNGERMSEGAGLASANA
                     APTNAALASGWNDLDFLLCHTPAKPATTITSSMIRLAAVQTEAPQAKTNAASPALAFA
                     DTDPPVWDGLGSLSYKVTTSSAAAQVYFDQGLRLTYAFNHEEAQRAFRKAQKLDPDCA
                     MCFWGEALVLGPNINMPMVEEAVAPAFAAMQKARALAAKASPREQALITALSARYVED
                     AKADRTKLDAAYAAAMANVALQFPEDNEIAVLYAESVMDLSPWDYWQRGGREPNPQSA
                     PIVPTLERVLARDPDHPGAIHYYIHAVEASDRPKRAEPYADRLRGAVPGAGHLVHMPS
                     HIYYRVGRYLDALGDNKTAMAVDETYLAANEVPMGVYRLGYYPHNVHFVMASALMAGD
                     GPSGITAAEKLRGLVPDQAALAIPGAHPVKAAPYFAHALFSPPDTILALPDPGNTIPY
                     VKAMWHYARGKAYVAQRDLGAATAEANAIETLERTADFSLLKTAGIPASDVLKLARSV
                     IEGRVALAQGDANKAIERFEQAAALQETLPYMEPPYWYYPVRQSLAVALMQAGRLDDA
                     EGQFRRALARAPNNGWSYYGLAELYKTRGDVEAARKAEAELAKTWVGDRQLLQLSKL"
     gene            complement(4662..5114)
                     /locus_tag="VM910_40210"
     CDS             complement(4662..5114)
                     /locus_tag="VM910_40210"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HVI68751.1"
                     /translation="MTAADLHCVRGYVLVVALVLGAAQTAAADGLRAGQWRTVNSPEI
                     DGVAGPVQQSIRCLTPEAVADLDKTFSPISRTTNSTCERVEHESTPQRLKWRLQCTGQ
                     IDMDVAGEFTFDSPEHYTATVTARSFMLGRLMQSVRTSIEGQRVGDCP"
BASE COUNT          953 a         1643 c         1689 g         1026 t
ORIGIN      
        1 ggctgattgg ctccgcgtca cattgcccgc ggtcatcctc gcgaaagcga ggagccatat
       61 tccagaggct gtggttatgg gtttccgctt tcgcggggac gacgatttat cagttgatga
      121 gtcgaagtag gtatcgtctc agtgtcggga ggcgcgcgcg ttgcggcgag cgaggcacgg
      181 accaacttca tcctccgatc tgcgggatct tcgcgtcggc gatcaccttc ttccacttgc
      241 caacgtcggc cttgatgtag gcggcgaacg cagccggcga cattccatcc ggttcgaggc
      301 cgatcgccag cagcttgtcc ttcacgtcgg gcaggttgat gatgcgtgcg atttccgtct
      361 gcagcagatc gacgatcgat ttcggcgtgc ccgcaggcaa cagcacgccc tggatggtct
      421 cagcctcttg gtttttgatt ccgagttcgt cgagcgtggg cacgtcgggc aaagccggcg
      481 agcgggtcgc cgcggtgacc gcaagcgcgc gcagcttgcc gtccttcacg agcggggtcg
      541 cgggcggaat cgcctggaag cacatcggcg tgtgcccagc cacgaccgac tgtatcgacg
      601 gaccgccgcc cgggaacggc gcgagcgcga aatccagtcc gaaggtgagc ttgaacagct
      661 cgctcgaaag atgcggcgtg gtgccgatac caggtgaggc gaaggtgtac tttccgggat
      721 tggccttgat gagttccacc agctccttca ccgatttggc cggaatgctg ggatgcacga
      781 acaggccgtt gggagaaccc gcggccttgg tgacgggcgc gaagtccttg tcgggatcgt
      841 aaggaggctt ggcgtagagg ctcggattga cggtgtagct cgacgacacg aacaggatcg
      901 tgtagccgtc ggccggcgcc ctggcgccgg cgcccatgcc gagattgccg ccggcccccg
      961 ccatgttctc gatgtagaac tgctggccgt atttttccga cagcttgagc gcaatcagcc
     1021 gagccaccgc gtcggtcggc cccgcggccg cgaatggcac gatgatcttg accggccggt
     1081 cgggatatcc cgggctctgc gccagcgcgg cggtggcaaa gagaagcccg gcgcaggccg
     1141 cgagcacgcg ggcgatcacg ttggtcaatc tcatgggcgc tcccctggcc gtgtccggcg
     1201 ttatggcggg catccgcgtc tctctttctc gcgacggcaa ggaggtagat cctgccaaca
     1261 agcccggcca caatctggac ggcagcctgt cagtagcaga ctgacgaatg ggggtggcga
     1321 atgcaaacat ttcctggcgc gaccgaatgc ccaggcgctg cagcgatcgg ctccaacgcg
     1381 gcctgttggc gacaaggatg tcacgccgat ccgtgccgcc tccgcgatag gtttcgcggc
     1441 gacagctacg gcagctggga taccaacttg tgaaattccg actgcggcca aatgcgggcc
     1501 gtgcgggtcc gcacgctccc cttcgagccg agcgcgagcg cgaatttcgc cgcgttgtcg
     1561 ccgttggggg tatcgatgat gaccaggaga tcgctgtcgc cggacgtaag gtagacctcc
     1621 ttgatctcaa ctcccacttt cttggccagg tcttgagcgg ctttggcgcg cttgggagca
     1681 tccttgatcg cccgtattcc ttgctcggtc cagctcagcg aacacatgaa cgtcggcatg
     1741 cgatcctccg tgtgattgcc cgcaaagatt gccgaccgct gcagaagcgg ccgtcgatcc
     1801 gtggactgtg aaagcgtgat ggagacgcca aaaaagcggc ggtctgccaa gagatcgtgc
     1861 ccgccgacgg cactatcgct gcctgttgac ccgaacggaa tttcggcacg ttccgttaca
     1921 aagggcagct catcaaatgt tacaccgctc gcccagcctg cggaaggcgg gagaagaagg
     1981 aacgctcgcg cgggattcga cggatctaat tcccgctcgc tgcgaggccc tcgatcatcg
     2041 cttggcaatg ttcagataaa ttggcaaacc ggcggcaaat ggcctggacg gctacgacca
     2101 cttggctccg cggcgccaag ctgaaaaact tttcggtctc ctcggtgatg gcttttagta
     2161 attctgcgtg attgaccgga agacgtgggc aataactcgc ttgaaatttt ctgaatgcgt
     2221 cctcggcatg atcggccgag gtgtaagcaa ccaaacagag agaattggtg ttgacccagc
     2281 tttggtcctt gctgcaagtg agccttctgc tcgtgatctc gccccgcccc gcactgttag
     2341 cgctgcgtac gatcctgaga ttcggacact cgcaaaccgt cgatcccaga ctgaacgttt
     2401 gacctgcata ttcgcaggtt tgcgccgacg ccgttgcacc gcacagagcg agaacacaaa
     2461 tcagcgcgag aaaacctgca atcttgggca ttgctgccct ccgtgcgtcg gaagcccgaa
     2521 taggagttga gctccggccg aagaaagcgg acgccgaccc ttgtcatcgt ttccgattcg
     2581 ccgatcgctg gccagtcaaa aatcgagtag ctgcaagtat ctaccgattg tcgttatgtc
     2641 gccggtcgca ggtaaaaagg cggatgcgcg tcacagcttg gacagttgca gcagttggcg
     2701 atcgccaacc caggtcttcg cgagttctgc ttccgccttg cgcgccgcct cgacgtcgcc
     2761 ccgcgtctta tacagttcgg cgagcccata ataggaccag ccgttattcg gcgcacgcgc
     2821 aagggcgcgc cggaactgac cctccgcatc gtcgagtcgg ccggcctgca tcaacgcgac
     2881 cgcgagcgat tgacgcaccg gataatacca gtacggcggc tccatatagg gcagtgtctc
     2941 ctgcaatgcc gccgcctgct cgaatcgctc gatcgcctta ttggcgtcgc cttgcgccag
     3001 cgcgacgcgg ccttcgatca cgctccgcgc aagcttgagc acatcggatg cgggaatgcc
     3061 cgccgtcttg agcagcgaga agtcggccgt gcgctcgagg gtttcgatgg cgttcgcctc
     3121 ggcggtggcc gcgccgagat cgcgctgcgc cacatatgct ttgccgcgcg cgtagtgcca
     3181 catcgccttg acatatggaa tggtattgcc gggatcgggc agcgcgagga tggtgtcggg
     3241 cgggctgaac agggcgtggg cgaagtacgg cgctgccttc accggatgcg cgcccgggat
     3301 ggcgagtgcg gcctggtcgg ggacgaggcc acgcagcttc tccgctgcgg taatgcctga
     3361 gggcccgtcg cccgccatca gcgccgaagc catgacgaag tgcacattgt gcgggtaata
     3421 gcccagccgg tagacgccca tcggcacctc gttcgcagcc aggtatgttt catccaccgc
     3481 catcgcggtc ttgttgtcgc cgagggcgtc gagataacgg ccgacgcggt aatagatgtg
     3541 gctcggcatg tgcaccaggt ggcccgcacc gggaacggcg ccacgcaacc ggtcggcgta
     3601 gggctccgcg cgctttggcc ggtcggaggc ctcgaccgcg tggatgtaat agtgaatcgc
     3661 gccgggatga tcgggatccc tggcaagcac gcgctccaaa gtcggcacga tgggcgcgct
     3721 ttgtgggttc ggttcccgtc cgccccgctg ccagtagtcc catggactga gatccatgac
     3781 cgattccgcg tacagcaccg cgatctcgtt gtcctccggg aattgcaacg cgacgttggc
     3841 catcgcggcg gcataggctg catcgagctt tgtccggtcc gccttcgcgt cttcaacgta
     3901 tcgcgccgac aaggccgtaa tcagcgcctg ctcgcgcggg cttgccttgg cggcgagcgc
     3961 ccgcgccttc tgcattgcgg cgaaggcagg cgccaccgcc tcttcgacca tcggcatgtt
     4021 gatattgggg ccgagcacga gcgcctcacc ccagaagcac atggcgcagt cgggatcgag
     4081 cttctgcgcc ttacggaatg cgcgctgcgc ctcctcgtga ttgaacgcat aggtcagccg
     4141 cagtccctgg tcgaaataga cctgcgccgc ggcgctcgaa gtcgtgactt tgtaagagag
     4201 cgagccgagg ccgtcccaca ccggcggatc ggtatcggca aacgccagcg caggcgacgc
     4261 ggcattcgtt tttgcctgcg gcgcctcggt ctgcaccgca gccaagcgga tcatgctgct
     4321 cgttatggtg gtggcaggct tggcgggcgt gtggcagagg aggaaatcca gatcgttcca
     4381 tccggaggcc agcgctgcat tcgtcggggc ggcattcgcc gacgcgagtc cggcaccttc
     4441 tgacatgcgc tcgccgttgg agatcaacaa cgcgagcccg cctgcgaacg ccgctgtaca
     4501 ggcggtgaaa agcgacatcc gcgaccgcat caacgccttc atgatgtcct ccgatcaaag
     4561 cgcgaggacc gcgtccatgc gtggttcact tggctgacgc ttgcaattgg gaccctcggc
     4621 gacctgaggg ttagcgcagt cgccctccat cccgcaagcc gctacgggca atcgcccacg
     4681 cgctggcctt cgatcgacgt gcgtaccgat tgcatgaggc ggcccagcat gaatgagcgc
     4741 gcggtgacgg tagccgtgta gtgctctggg ctgtcaaacg tgaattctcc cgccacgtcc
     4801 atgtcgatct ggcccgtgca ttgcaggcgc catttcagcc gctgcggcgt gctttcatgc
     4861 tcaacccgct cgcaggtcga gttcgtcgtg cggctgatgg ggctgaacgt cttgtcgaga
     4921 tcggcgaccg cctccggcgt cagacagcga atgctttgtt gaaccgggcc cgcgacgcca
     4981 tcgatttcag gggaattaac cgtcctccac tggccggcgc gcaatccgtc ggcagcggca
     5041 gtctgggcgg cgccaagaac gagcgccaca acgagcacat agccgcgcac gcaatgaaga
     5101 tccgcagcag tcatttatcc gatagtcccg atggcaaagg tgtatcaggg tggatgataa
     5161 cagtgtcctc ggtcgcgtcg gaaggccgtt gccccgtcct cagacgcgct gaaaatcatc
     5221 cggttgtgaa ctgtcattgc gccttgatgt tggccgcgcg aatgaccttc gcccacttgt
     5281 cgatttccca tcggatccgg tcggcgaatt c
//
LOCUS       DATLJT010001782         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1598418,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001782 DATLJT010000000
VERSION     DATLJT010001782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598418"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VM910_40215"
     CDS             complement(<1..921)
                     /locus_tag="VM910_40215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68752.1"
                     /translation="MMRYSCVMCLVAIATVSLAPDRAFSQTPSPPLLQLETKIPLGDV
                     KGRIDHMAIDLPRRRLFVAELGNDTVGVVDLNEQKVQHVITELKEPQGVGYVPSSDTV
                     FVTNAGDGSVLLFRGAEYAAAGRIDLGNDADNIRVDSASNRVFVGYGSGALAVIDPAT
                     NGKIADISLQAHPESFQLARSDRRIFVNVPKAREIAVVDRFASKQTGSWPIDNGSNFP
                     MALDESSGRVFVASRNPPQLSVFSVRDGSAIAAVGACGDTDDLFFDAKRQRVYLSCGD
                     GYVDVFEAQGDAYGRSAHIPTISGARTALFV"
     gene            complement(963..1514)
                     /locus_tag="VM910_40220"
     CDS             complement(963..1514)
                     /locus_tag="VM910_40220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68753.1"
                     /translation="MMRRSLMLVIAVGLATLGSSIAWSADSDQAALIKALDGAKLTLL
                     QGIAQVAKGTEVPIEAKYQMVRGKLILSIYTSVKGFDTAAEDNSFNEYIGDVTTANWT
                     PKKDVFADLKHIARSAQYHALLSMTKVRIPTIIQKASAQGTVLAVREKIRGGKPVFEV
                     MVVQDNTIRPTFYDLATGEPTAG"
     gene            1896..2573
                     /locus_tag="VM910_40225"
     CDS             1896..2573
                     /locus_tag="VM910_40225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVI68754.1"
                     /translation="MRLLVVEDEPRILEIIKAALERAEFAVDTVRRRADAREALANTA
                     YDAAILDLGLPDGDGLNLLAELRPTGNRIPILILTARDAVEDRVLGLDAGADDYLVKP
                     FAMSELVARTKALLRRPGAALGTTLKAGNIVFDTIGRDVSVAGIPLQLPRREAAILEH
                     LLRRQGRVVPKSVLEEKLYGIDEELESNAVPVHVHHLRRKLQEAAASAEIHTVRGVGY
                     LLAETKG"
     gene            2570..>3931
                     /locus_tag="VM910_40230"
     CDS             2570..>3931
                     /locus_tag="VM910_40230"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVI68755.1"
                     /translation="MSFVGFRSLQVRLAVRLGVLYVAAAAIAVGVLAYQAYDTAGSLN
                     DRELGLRAEDLARAMVADGAGQPRLELPSKLAAAYAAAPEDDLFAIRDTNGRVIAASP
                     AEFGERVSKWPPSADEPSYFRLSGAGSEDFGSETYYGLSVALQTAAGPMWISVARTEG
                     SDALIKSILREFVFDAIWVSPLLMLATLAIGVFAIRNGLKPVREISRMASSIGPDATS
                     VRLPEQNLPTEIMPLVASINHALDRLEKGFAVQREFTANAAHELRTPLTIITSALETM
                     DGNSELEKLRTDVARVNRLVEQLLRVARLDAVALEFGTVDVNKVASSVVAAMAPWAIA
                     QGKPIAFAGTEWPVNVKANAHALEDALRNLIENAVVHAPPGTEVTVTVDRGGRIEVAD
                     RGSGVPPEDRENIFKRFWRGAGERKEGAGLGLAIVGEIMRAHRGSVSVADNPGGGALF
                     TLSF"
BASE COUNT          752 a         1188 c         1184 g          807 t
ORIGIN      
        1 aacaaacagc gcagttcgtg cgccggatat cgtcgggatg tgggccgagc ggccgtaggc
       61 gtccccttgc gcctcgaaca catccacgta tccgtcgccg cagctgaggt acacgcgctg
      121 gcgcttcgca tcgaagaaga gatcgtcagt atcaccacac gcacccacgg cggcaatcgc
      181 gctgccatcc cgcacggaga acacgcttag ctgcggggga ttgcgagacg ccacaaaaac
      241 tcggccggaa ctttcgtcca gagccattgg aaaattgctg ccgttgtcga tcggccagct
      301 ccctgtctgt ttgctcgcaa aacggtcgac gaccgcgatc tcgcgcgcct tcgggacgtt
      361 cacgaagatg cgacggtcag agcgggcaag ctgaaagctt tcgggatgag cctgcaatga
      421 aatgtcggcg attttgccgt tcgtcgcggg atcgatcacc gccagtgcgc cgctcccgta
      481 cccaacgaac actcggttgg acgcgctatc cacgcgaatg ttgtcggcat cattgccgag
      541 atcgattctg cctgctgctg cgtattccgc gcctcggaac agcagcacgg acccgtcacc
      601 cgcattggtg acaaaaacgg tatcgctcga cgggacatag ccgacacctt gcggctcctt
      661 caattcggta atgacgtgct gtaccttttg ctcattcaaa tcgacgactc cgacggtatc
      721 gttaccgagc tcggccacaa acagacgtcg ccggggcaga tcgatcgcca tgtgatcgat
      781 ccggccctta acgtcgccca acggaatctt agtttcgagc tgcagcaacg gggggctcgg
      841 cgtctgagag aatgcccgat cgggtgcaag cgacaccgtc gcaattgcca cgaggcacat
      901 cacgcaagag taacgcatca tcattggcta tcccttctgt tcattgggtc cagactgctt
      961 tctcagccgg ctgtcggctc cccggtggcg aggtcgtaga acgtcggcct gatcgtattg
     1021 tcttggacca ccatcacttc gaaaacaggc ttgccgccgc gaattttctc cctaacagcc
     1081 aagaccgtgc cctgcgccga agccttctgg atgattgtcg gaatcctgac cttggtcatc
     1141 gagagcagcg cgtgatactg cgccgaccga gcgatgtgct ttaggtcggc gaacacgtcc
     1201 ttcttcgggg tccagttcgc tgtagtcacg tcgcctatgt actcgttgaa ggaattatct
     1261 tccgcagcgg tgtcgaagcc cttcaccgag gtatagatcg acagtatcag cttgccgcgt
     1321 accatctggt attttgcttc gatgggcact tcggttccct tggcgacctg agcgatgcct
     1381 tgcaacaggg tcagcttggc gccgtccaat gctttgatga gagctgcctg atcggagtct
     1441 gcagaccagg cgatgctgct tcccaacgtc gccaatccga ccgcgataac gagcatcaga
     1501 cttcttctca tcatgttgac cttcttatgt acttgcgagg gaaaagcgca ttcgcggacc
     1561 agctatttga ccatgaccca tgttggctgc agcagtggga atgcgatgag gccgaccaca
     1621 gccgtcgccg ctatgagccg cgggctattc acgtgcctcg ccctgatgct gcgggggttc
     1681 gtttaaggca ggtttatgcc gaattgggcg ggatgatcgg cggcagtcgc aagtccgaga
     1741 gctgtatgtt gtttccaaag attctggata ccgtgcgcgc agctgcacca tcccaggcgg
     1801 cttaaaactg tcttaaactt cgcatgtgtt gagcagggcg cgcgctggcg cagtcaactg
     1861 aaagtaggcg tgccggtttc cgaaagggca agcacatgcg tctccttgtc gtcgaagatg
     1921 agccgcgaat tttggaaatc atcaaagctg cgctggagcg cgccgagttt gccgtggaca
     1981 ccgtgcggcg gcgggccgat gcgcgggaag cgctggcaaa caccgcctac gatgccgcaa
     2041 tcctcgacct cgggcttccc gatggtgacg ggctaaacct gcttgctgag ttgcggccta
     2101 ctggaaaccg catccccatt ttgatcctca ccgcgcgcga tgcggtggag gaccgtgttc
     2161 tcggcctcga tgcgggtgca gacgactacc tcgtgaagcc attcgcgatg agcgagctcg
     2221 ttgctcgcac gaaggcgctg ctgcgccgtc ccggagcggc gctcggtacc acccttaagg
     2281 ccggcaatat cgtcttcgac accatcggtc gagacgtcag cgttgccggc attccattgc
     2341 aacttcctcg tcgtgaggct gcgattttgg agcacttgct ccgccgccag ggccgcgtcg
     2401 taccgaagag cgttctcgaa gaaaagctct acggcatcga cgaggagctc gaatcgaatg
     2461 ctgttccggt gcacgtgcac cacctgcggc gcaagctgca agaagccgca gccagcgcgg
     2521 aaattcatac tgtgcgcgga gtaggttatt tgctcgccga gacgaaagga tgagttttgt
     2581 cggttttcgc tcgttgcaag tgcgcctcgc ggtgcgcctt ggggtgttgt atgtcgccgc
     2641 cgctgcaatc gcggtcggcg ttttagccta tcaagcctac gacacggcag gatcgttgaa
     2701 tgaccgcgaa ctcggcctgc gcgctgagga tctcgcacgg gcgatggtcg ccgacggtgc
     2761 cggccagcct cgccttgagc tgccctcgaa gcttgctgcc gcatacgcgg cggcacccga
     2821 agatgacctg ttcgcgatcc gtgataccaa tggccgcgtc attgccgcat cccccgccga
     2881 gtttggcgag cgcgtgtcca aatggccccc ctccgcggat gagcctagct atttccgcct
     2941 cagtggcgct ggttccgagg attttggctc cgagacttac tacggcttga gcgtcgcgct
     3001 gcagacggcc gcgggtccga tgtggatatc ggtcgcgcgg acggaaggat cagacgccct
     3061 catcaaatcg attttgcgcg aattcgtatt tgacgccatt tgggtgagcc cgctgctcat
     3121 gttggccaca ttagcaatcg gcgtctttgc aatccgcaac gggctcaaac ccgtgcgcga
     3181 gatttcccgt atggcgtcgt cgatcggtcc tgatgcgacg tcggtacggc tgccggaaca
     3241 aaacctgccg acggaaatca tgccgctggt cgcttccata aaccatgcgc tcgatcggct
     3301 cgagaaaggc tttgccgtgc agcgcgagtt taccgccaac gccgcccatg agcttcggac
     3361 gccgctcacg atcatcacct cggctctcga aacgatggat ggaaacagcg agctcgagaa
     3421 attgaggacc gacgtcgcgc gcgtgaacag gctcgtcgag caactccttc gcgtcgcccg
     3481 gttggacgcc gtcgcgctgg aattcggtac cgtcgatgtc aacaaggtcg cgtcctcggt
     3541 tgtggccgcg atggcgccct gggccatcgc ccagggtaag cccattgcgt tcgccggaac
     3601 cgagtggccg gtgaacgtga aagcaaacgc tcacgcgctc gaagacgcgc tgagaaatct
     3661 gatcgagaac gcggtcgttc acgcgccgcc tggaacggaa gtgaccgtga cggtcgatcg
     3721 tgggggccgg atcgaagtgg ccgatcgcgg cagcggcgtg ccgccagagg atcgcgaaaa
     3781 cattttcaaa cggttctggc gcggcgcggg tgaaaggaag gaaggcgccg gcctcggcct
     3841 cgccattgtc ggcgaaatca tgcgagcgca tcgcggcagc gtcagcgtcg cagataatcc
     3901 cggcggcggc gccctcttca ccctgtcatt t
//
LOCUS       DATLJT010001783         2618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_682171,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001783 DATLJT010000000
VERSION     DATLJT010001783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682171"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..943
                     /gene="hflK"
                     /locus_tag="VM910_40235"
                     /pseudo
     CDS             1..943
                     /gene="hflK"
                     /locus_tag="VM910_40235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974515.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
     gene            1351..1713
                     /locus_tag="VM910_40240"
     CDS             1351..1713
                     /locus_tag="VM910_40240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68756.1"
                     /translation="MTEPGLQRDGKNEATMTSTLTPPPLIMVNKLRAPVADLSPTKRS
                     AVIECFNNNGLFKRNGYWWGAPEGKHISGVTVADLARDGIFSVITNLRNSSARLTERG
                     QWFARTLVDTANGVHVRE"
     gene            2341..2607
                     /locus_tag="VM910_40245"
     CDS             2341..2607
                     /locus_tag="VM910_40245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68757.1"
                     /translation="MTKESEHSANLSKAREMLLVERRQLAAELTKSYQRGDGEKRERF
                     MVVQSMIEAIDRAALDEARIARGEDPVPTVTHYVTGKPSQIPGQ"
BASE COUNT          601 a          772 c          793 g          452 t
ORIGIN      
        1 ctgacgaggt cggcgcggtg cagcgattcg gaaagttcgt ggcgccggag gtgcagccgg
       61 gcctgaacta ccacctgccc tatccgatcg agaccgtgct gacgccgccc gccttgcggg
      121 tcaacaagac cgacattggc atccgcccgg ccgaggagtt tggccgccgc ggtgcgcaac
      181 cgcgcgatct accggaagaa agcctgatgc tgaccggcga ccagaatatc gtcgacgtcg
      241 agttctcagt gctttggcgg atcaagcccg gcaacgtcaa cgactatctg ttcaatgtgc
      301 aaaatcccga aggcacggtg aaagcggtcg ccgaaagcgc gatgcgcgag gtgatcgggc
      361 gcttgaacgt ccaaccgctg ctcaccggcg gctcgaaagt gacgcaggat aaaacagaaa
      421 cctcggtgca ggatctggtg cagaccgtgc tgcagaagac gctcgattca tataatgcgg
      481 gcatcctcat ccagaacgtg cagatctcgc ggatcgatcc gccggccgag gtgatggatt
      541 ccttcctgga cgtggcggcg gcgcggtcgg acgccgagcg cgcgcagaac gaggcgcaaa
      601 cctacgccaa ccgggtggtg ccggaagcgc gcgggcgcgc cgccaagatc ctgcaggacg
      661 ccgacgccta tcgcgagcag gcggtcgccg aagccaaggg cgagacttcg ccttcctgca
      721 gatctacaag caatacaaaa aagcgccgac ggtgacccgc gagcgcatgt atctggaaac
      781 gatggaacgc atcctcagca acaccaacaa gacgatcatc gataccggcc acagcggccc
      841 gggcgtggtg ccctacctgc cgctgaccga tctgccggtg ccgccgagcc gcaccagcac
      901 gccgccgcga gtcggcactt cgtcgacggg aggcgcgcaa tgaggatcgg tcaacggcgc
      961 cagtatcgat ccgcgcgatg cgctttctcg gcctcggcgt cggcgtgatc tcgatctcac
     1021 cctcggcgac taaggcgtct tccaacagct ttttgacgac ggcgctgacg gggacgtcgt
     1081 cacgcgcggc gacgcggcgc agagcgcggg cccagaaggt atatcgggga taaatgccac
     1141 aaagtgacac cctcactgat acggagcgcg gccatcgatt gtagccttta tgcgtcggat
     1201 ttcgcatggt cttggcggcg ctgggtatgc ccgattgaac caggtcaacc taagcatgca
     1261 cgagcgaacg ctccgatttt agagcattca acgatcgtcg ctccaggtcg cagcgataca
     1321 gggatgattt gtcaatcagg ggacaagaca atgacagagc cgggactaca acgagatggc
     1381 aagaacgaag ccacgatgac atccactctc acaccgccac cattgatcat ggtgaataaa
     1441 ctgcgcgcgc cggtcgccga cctgtcacct accaagcgaa gtgccgtgat cgagtgcttc
     1501 aataacaacg gtctttttaa acgcaatggc tactggtggg gagcgccaga aggcaagcat
     1561 atctcagggg tcactgttgc agatctcgct cgcgatggca tattctcggt aataacaaat
     1621 ctccgaaata gttcggcacg gctgaccgaa cgtggccaat ggtttgcacg gacgctggtc
     1681 gacactgcaa acggggtaca tgtgcgggaa taatcgccac gttgacgata aatagatgcg
     1741 cggtagcggg ggctcagcgc tctttccgat agccccctcc attccgcgcc tccttccggc
     1801 gcgcacggca aacggcgaga gccaacctgg cccgcgctgc agcccgcgtt agcgttttga
     1861 gaaaaagcgt accgagggcg caaccaggca aaaggcgttc agtatgggaa tctcgctcga
     1921 ctcaagggct tgatacttgc gcggttcact tcaagggcga acgtttgcag atcaagaggt
     1981 cgaacgaggt tatccttcgc aagcgaatat acgtttagac cggggctccg cttcatcgtt
     2041 gtggcgcacc gcggagcgct tgcgctcgat gcgggaagcg gtcctctatc tctgtgtaga
     2101 ccttatgcag ccattgttcc gcgcgctcgg cctggcggct tgccagatca aatccaggta
     2161 gaagtggacc cagagatcga agtgtctggg aagaccgtcg gcaaacttcg taaaatcaca
     2221 gcgaggcccg attaatttgg caattacgac gccgcgggac cgcttgataa tccgcagccg
     2281 caggctagtt tcaccgccga agcgcggcgc attgcaatat tgcaaccgaa gagggctccc
     2341 atgaccaaag agagcgaaca ctcggccaat ctcagcaagg cgcgagagat gcttttagtg
     2401 gagcgccgac agctcgccgc cgagctaacc aagtcctacc aaagaggcga cggcgagaag
     2461 cgagaacgct tcatggtcgt tcaatccatg atcgaagcga ttgatcgagc ggcgctagac
     2521 gaagctcgga ttgcgagagg agaggacccg gtgccgaccg taacgcacta tgtcactgga
     2581 aagccaagtc aaataccagg gcaataaatc ctgccgcc
//
LOCUS       DATLJT010001784         3655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2060256,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001784 DATLJT010000000
VERSION     DATLJT010001784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060256"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(659..735)
                     /locus_tag="VM910_40250"
     tRNA            complement(659..735)
                     /locus_tag="VM910_40250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(699..701),aa:Met,seq:cat)
     gene            complement(929..1672)
                     /locus_tag="VM910_40255"
     CDS             complement(929..1672)
                     /locus_tag="VM910_40255"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVI68758.1"
                     /translation="MLFRVGRTFAVVFCVGGVAIEFAAAADIPYVQSPAPAYVPPPSW
                     AGFYLGGQVGYGMDSVRWRNLGGSAFFSPPNSLTRDRGSGVIGGGQLGYNFQYDRLVF
                     GIEGSVSAADFDRSFASPYFPATDVWSSRLTWLTTVTGRVGYGFDNWLPYIKGGFAAG
                     NVETSIQANPFGVMSQGSAVHSGWTLGGGVEFKVAPQFSLGLEFMHTDLGRSTDINGP
                     QIVPGAPESYGVGLRSNSIMARFNYFFGR"
     gene            complement(1855..2517)
                     /locus_tag="VM910_40260"
     CDS             complement(1855..2517)
                     /locus_tag="VM910_40260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68759.1"
                     /translation="MNKLRQWRLVGSAVGGERHFWLDKIVFGLDRVLRRWQSVVEFTH
                     DPTCILRITLGRLDQDFVLADGTAGRAGDRFVDLHLWNEQIPAMPKEGASIAWARQMS
                     LCFQHSLRQLARYLTLRPDLDDISLLRCTMGFAGRERNDQMVRLIRRYGFEFVPPATT
                     MTLGERARRLGENIMISLIVLARNQAALRRDTLRRGRTRVFMSRKVLEQRYGGGAGRI
                     DL"
     gene            complement(2845..3549)
                     /locus_tag="VM910_40265"
     CDS             complement(2845..3549)
                     /locus_tag="VM910_40265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68760.1"
                     /translation="MLELRDVVCCYGQVTALKGVSLTVGKGQLVALIGANGAGKTTTL
                     KAISGLLHPTAGTIVFDGHDITHASARTILALGLAHCPEGRHVFAQMTVLENLEMGCY
                     LRTDRRGIAADMTRVFDQFPRLAERRQQAAGTLSGGEQQMLAIGRALMSQPKLILFDE
                     PSLGLAPNIVERTFDIVTNIRASGTTVLMVEQNASAALDMCDHAYLLESGSVAIEGSG
                     RELIDDPHVREAYLAG"
     gene            complement(3542..>3655)
                     /locus_tag="VM910_40270"
     CDS             complement(3542..>3655)
                     /locus_tag="VM910_40270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68761.1"
                     /translation="RIVVLNYGRIIAEGPPAAIQKNPDVIRAYLGEGKKRA"
BASE COUNT          767 a         1145 c         1056 g          687 t
ORIGIN      
        1 gcccctcaac agccagtctg ccgacaaacc gggaacctgc tccagcaggg tgagcgttag
       61 gttttggctg aggcccgacc cgattttgcg ttgtgccacc ccgcgaatcc tattttgagg
      121 cgacgggcca ttctcgtggt ctcgccggca cgagcatatc ggggacgccc gttgtggctg
      181 cggcggtgcc tagcgagagc agtccaaatt ggcgacgatg cattcttacc tcctccaacg
      241 cggcgcattt gctctaattc acaaggcgct cagagcgccc gcgccttggt aagcgataaa
      301 ccagatcgac ggctcaagct gagaaggtgg gaggctacga cgagacgggg accatcgaat
      361 gtctcaccag ccgccgcgaa ggtactcttc caagcattga ggcgatggct ggacgcaaaa
      421 aatctttgcc aaatgttacc ccatttgtta cccgttgaaa taattggcgc acccgaagag
      481 attcgaactc ctgaccccag atgtctgagc tcaggcaaga tggattcttt gttgctcgtg
      541 ccagacacca gaatttaatg tatcttcggg ggccccaagt gtgtacctgg ccgtgcaccc
      601 tactactatt ctgctgtctc gccgtaagat ttagcgggct ataagcactt gattttgttg
      661 gtagcggggg ccgggatcga accggcgacc tacggattat gattccgccg ctctgaccag
      721 ctgagctacc ccgccaacaa tttcaacgac ttacagtgcc gcccgagaag ctcgcaagcc
      781 tttttgcacg ctggcgacct cgcgggccgg caccggctat gtagtcgagg gagatagacc
      841 gagcaagccg cgggaatggc gcagatgatt ggctggcacc tgccgcctct cgcctcactc
      901 aaagccattc agatttggtg ccgccgcgtc atcggccgaa gaagtagttg aaccgagcca
      961 tgatgctgtt gcttcgcagt cctacgccat agctctccgg cgcccccgga acgatctgcg
     1021 gtccattgat atcggtgctt ctgccgagat cggtgtgcat gaattccagg ccgagggaaa
     1081 attggggcgc gactttgaac tcgaccccac cgcccagcgt ccagcccgag tgtaccgccg
     1141 atccttgcga catcacgcca aacgggttgg cctgaatgct ggtctcgaca ttgccggcgg
     1201 cgaagccgcc tttgatatag ggcagccagt tgtcgaagcc gtagccgacg cggccggtca
     1261 ccgtggtgag ccaggtgagc cgcgaggacc acacatccgt cgccgggaaa tacgggctgg
     1321 caaagctgcg gtcgaaatcg gcggccgata ccgacccctc gatgccgaac accaaccggt
     1381 cgtactgaaa attatagccg agctgcccgc cgccgattac cccgctcccg cggtcgcgcg
     1441 tcagcgaatt cggcggcgaa aaaaatgccg aaccacccaa attccgccac cggacgctat
     1501 ccatgccgta gccgacttgg ccacccagat agaatccggc ccagctcggc ggcggcacgt
     1561 aggcaggcgc gggactttgc acgtagggaa tatcggctgc tgcagcaaat tcgatcgcca
     1621 caccacccac gcaaaagacg acagcgaacg ttcgaccgac gcggaaaagc atatggcctc
     1681 tccctggtcg cccccaacac gacattttgc ggaaacttgc ccgcgacgtt ggcgaaagcg
     1741 cggcttcgat acgtctcctt tccgacaagg ttaataaatt ctgcggcgct tcactcgagc
     1801 gggaccttat caaggcacca tcttcgtcgg tcaggatggg caggttgggc gatgctacag
     1861 atcgatgcgg cccgcgccac ctccatagcg ttgttcgagc acctttcgcg acatgaaaac
     1921 ccgcgtgcgg ccccggcgca gcgtgtctcg ccgcagcgcc gcttgattgc gcgctagcac
     1981 gatcagcgag atcatgatgt tttcgcccaa ccggcgggcg cgttcaccca acgtcatcgt
     2041 tgtcgccggc ggaacgaact cgaatccgta tcggcggatc aagcgcacca tttggtcatt
     2101 acgctcgcgg ccagcgaacc ccatggtgca acgcagaagg gaaatgtcgt cgagatcggg
     2161 gcgcaaggtg agatagcgcg cgagttgccg cagcgaatgc tggaagcaaa ggctcatctg
     2221 ccgcgcccat gcaatcgatg caccctcctt cggcatcgcc gggatttgct cgttccacag
     2281 atgcagatcg acgaagcgat cgccggcgcg acccgccgtg ccatccgcaa gcacgaaatc
     2341 ctgatccaga cggccgaggg tgatacgcaa aatgcaggtc gggtcgtggg taaattcgac
     2401 gacggactgc cagcggcgca gtacgcggtc cagcccaaag acgatcttgt ccagccaaaa
     2461 atgacgctct ccccccacgg ccgacccgac cagtcgccat tgccgcaatt tattcatggc
     2521 atcggatgcc taacggcacg cttgctgcaa tattacaatc tattagtctg caccggcttc
     2581 caatctgcac caagccccca agccacgaac agcggtcgtc gtcattgccg ggcttgacac
     2641 cgcaagtcgg gtttacccga cttgcgacgc atagtactgg gcaacacggg caagcccgag
     2701 ttgcggtgca atccatctac ttcgtaagaa gatttttggc gaaagagatg gatgcgcggg
     2761 tcataagcgc gttcacgcgc gtcttcgacg cgcttctgcc cgcgtatgac gagtcgagga
     2821 taactgcaaa caggttaaac cgcgttaccc cgcgagatag gcctcgcgca cgtgcggatc
     2881 gtcgatcagc tcgcgcccgc ttccctcgat cgccaccgaa ccggattcca gaagataggc
     2941 gtgatcgcac atgtcgagcg cggcggacgc attctgctcc accatgagaa cagtcgtgcc
     3001 ggacgcgcga atgttggtga caatgtcgaa cgtacgctcg acgatattgg gagcaagccc
     3061 gagcgaaggt tcgtcgaaca agatgagctt gggttgcgac atcaaggccc ggccgatcgc
     3121 aagcatctgc tgctcgccgc ccgacaacgt tcccgccgcc tgctgccggc gctcagcgag
     3181 ccgtggaaac tgatcgaaga cgcgcgtcat gtcggcggcg atccctcgcc gatccgtgcg
     3241 caagtaacag cccatttcca gattttccag caccgtcatc tgcgcgaaca cgtggcgtcc
     3301 ctccggacaa tgcgcgagcc cgagcgcaag aatggtgcgc gcggaggcgt gcgtgatgtc
     3361 gtgtccgtcg aacacgatcg tgccggcagt cggatgcaac aatcccgaga tcgccttcaa
     3421 ggtcgtcgtc ttgccggctc cgttcgcgcc gatcaaggcg acgagctggc ctttgccgac
     3481 ggtgagcgat acgcccttga gcgccgtcac ctggccgtag caacagacga catcgcgcag
     3541 ttcaagcacg cttctttccc tctcccaaat aagcgcgaat aacgtcggga tttttctgga
     3601 tggccgccgg cggcccttcc gcgatgatgc ggccgtagtt gaggacgacg atgcg
//
LOCUS       DATLJT010001785         7061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4584648,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001785 DATLJT010000000
VERSION     DATLJT010001785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4584648"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VM910_40275"
     CDS             <1..745
                     /locus_tag="VM910_40275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVI68762.1"
                     /translation="CPADTPEVNVSEIELQGATVYRVNGLIDDCGKIVGEGKAKAGWF
                     DTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGLIGMWKAFAELEAIGF
                     IGSKRPRMVAVQAAGCAPMVRAFENGSEHAPRWEDAHTIASGIRVPQAVGDFLILRAV
                     RESKGFAIAVADEKISAALNDVAREEGLLLCPEGAATYAAYQQSLADGRVSKSDRVML
                     FNCATGLKYPLPPVTRTLDRFKPIDYAKL"
     gene            773..1744
                     /locus_tag="VM910_40280"
     CDS             773..1744
                     /locus_tag="VM910_40280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HVI68763.1"
                     /translation="MFKPVFAALIATLGLAGGASAEKYPSHPITVVVPFSAGGPSDAM
                     MRILAERMKTSLGEAVLIENVTGAGGSIGVGRVVHSAPDGYTIGFGHLGTHVANGAIY
                     KLNYDLVTDLEPVVRLPSNPMIIVGKNAVPAKTLPDLIAWLKSRPQPATAGTAGAGSG
                     AHVAGLAFENAAGVKLQYVPYRGTGPAMNDLVAGQIDIMVDQLSNSINQVRAGTIRGY
                     AVTDARRNDEAPDIPTTEEAGLPGFRMTLWSGLWAPKGTPKEIVAKLNSAVVDALSDP
                     QVRKALENLGLQVPSADQLTPEALGAWQKSEIAKWWPIIKAANVKVD"
     gene            1773..3005
                     /locus_tag="VM910_40285"
     CDS             1773..3005
                     /locus_tag="VM910_40285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVI68764.1"
                     /translation="MSLRSVTPSQVRRAFLLRDEIALLDVRHEADFATGHPLFAANMA
                     AERIVLEAEMRLPRKDVPIVLYDAGEGHVPKARETLAALGYTNVRELQGGLKAWRDAG
                     FELFQDVNSYAKAFGELVESRRHTPSLPADDVAALIASGANIAVLDVRRFDEYATMNI
                     PGSVSVPGAELVLRAGKAAPDPETTIVVNCAGRTRSIIGTQSLINAGVPNKVVALRNG
                     TIGWTLAKHALEHDADRRGAVGAFENASANARDVAYRAGVRRIGVGDLGVLQAQQNRT
                     LYRFDVRDEAEYTAGHIAGFRHYAGGQLVQEIDVAAPVRGARIVLTDNLGIRADMTAS
                     WLAQMGWDVYLLEGGYEGPLEVTPPLTMPKPDPAHRYRRPYEGTDASPAKMQAYLDWE
                     FGLVEQLRRDGTHGFFVI"
     gene            3032..3916
                     /locus_tag="VM910_40290"
     CDS             3032..3916
                     /locus_tag="VM910_40290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVI68765.1"
                     /translation="MPYAMTDDGVRLYFEETGSGRPVIFVHEFAGDLRSFEPQMRHFG
                     KRYRAIAFNARGFPQSDVPEHVSSYSQIRAADDILAVLEHIGERQAHIVGLSMGGFAT
                     LHFGLRHPQRALSLCIGGCGYGAEPDKRETFRAEADVIARALRTEGMAAFAERYALGP
                     TRVQYQNKDPRGHAEFKAMLAEHSAVGSANTQQGVQKERPSLYTLVEEMKRITVPALI
                     IHGDEDWPCLLPGVLMKQSIPSAALAVMPNCGHAINLEEPDEYNIIVGNFLAQVDSGR
                     WPQRDPRAVSASITGMKD"
     gene            3933..5597
                     /locus_tag="VM910_40295"
     CDS             3933..5597
                     /locus_tag="VM910_40295"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HVI68766.1"
                     /translation="MRGRRVLMDSLVMHGVRHIFGNPGTTETPLLDSLPAYPQIDYVM
                     TLHEGVAIAASSFYAQASGRTAVANLHVAPGLGNAIGALYGALKANSPVLVTAGQQDT
                     RMRLANPLLGHDLVAMAAPVTKWSVQVERADEIAPILRRAFKVATDQPAGPVFVSLPI
                     DALEQETSVEPVAPDRLWRCTQPDPKGIGEVASLLLKSASPVIIAGDDIARSRAEGEL
                     VGLVEAIGASLWFEGLRHHASFPTSHPNYRQSLPGDAVQVRNALHGADLVLLLGGPFF
                     EDIWYAAGGHFPAGAVVVQVEASPERLAFNHHLDAGVVGDLSTSLVSLTDAVRSRATP
                     EFRLAAQARNRALAELRAREKEAYRARLEKSWSREPSSMPRVAAELRRGLPDDAVIVD
                     ESITASLDLARSFDYRGFGDYFGGRGGGIGQGLAGAIGVKIAMPDRPVVAVSGDGSAM
                     YSIQALWTAAHHKLAIVFVILSNRQYRILKHNVDVWRQNFEAGTQHPYQNMDLTGPEL
                     DFVHLAAGMGVQGTRVEAADAIAPALADAVAANRPYLVEIAVEGKR"
     gene            5706..6599
                     /locus_tag="VM910_40300"
     CDS             5706..6599
                     /locus_tag="VM910_40300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HVI68767.1"
                     /translation="MPLLPFDELAQAIAGRRSVYGSISGLRLDRAGPGEAWSSLPYRP
                     VFVGDTETGFLHGGVVTAMLDESCGMAVQLALDGTRAIATLDLRIDYQKPATPGLDIK
                     AHSICYRVTRSIAFVRATAYQDSEEDPVATATACFMVGANRTNMLTDRPLDDGAPGLD
                     APEDPSGPFARSPFARCLGIRISEDGTLVMPFSPKIIGNPILPAIHGGMTGAFLETTA
                     IVGVTRELGVLALPKPIGLTINYLRSGRALASYANASIVKQGRRVIAFEAKAWQEDPA
                     KPIASAFGHFMLRKAPGGDEE"
BASE COUNT         1277 a         2188 c         2286 g         1310 t
ORIGIN      
        1 ttgtcctgcc gatacgccgg aagtgaatgt cagcgagatc gagctgcagg gcgcgaccgt
       61 ctatcgcgtc aacgggctga tcgacgattg cggcaagatc gtcggcgagg gcaaggccaa
      121 ggccggctgg ttcgatacct cgaccctcaa ggagccctat cgcatcgagg gcaagaagac
      181 gatggggctc gagcttgccg agcagctcgg ctgggacgtg cccgacgtaa tcttttatcc
      241 gaccggcggc ggcaccggcc tgatcggcat gtggaaggcg tttgcggagc tggaagcgat
      301 cggtttcatc ggaagcaagc ggccgcgcat ggtcgcggtg caagcggccg gatgcgcgcc
      361 gatggtgcgc gccttcgaga atggctctga gcacgcgccg cgctgggagg atgcacacac
      421 catcgcctcc ggcatccgcg tgccgcaggc ggtcggagat tttctgatcc tgcgcgcggt
      481 gcgcgagagc aagggttttg cgatcgcggt cgccgacgag aaaatttctg ctgcactcaa
      541 cgacgtcgcg cgcgaggaag gcctgctgtt gtgtccggag ggggccgcga cttacgccgc
      601 ctaccagcag agccttgcag acggccgcgt gagcaaaagt gatcgcgtca tgctgttcaa
      661 ttgcgccacc ggtcttaaat atccgctgcc gccggttacg cggacgctcg atcgcttcaa
      721 gccgatcgat tatgccaaac tctaggatgc actgatgcgg cagcgagttg ggatgttcaa
      781 acccgttttt gcggcattga tcgcgaccct tggcttggca ggcggcgcgt ccgccgaaaa
      841 atatccgagc catcccatta cggtcgtggt gccgttctcg gccggcggtc cgtcggatgc
      901 catgatgcgg attctcgccg agcgcatgaa gacgtcgctc ggcgaagccg tgctgatcga
      961 aaatgtgacc ggggcgggcg gctcgatcgg cgtcgggcgg gtggtgcatt ccgcgcccga
     1021 cggctacacc atcggcttcg gccatctcgg tacgcatgtc gctaacggcg cgatctacaa
     1081 gctcaactat gatctcgtca ccgacctcga accggtcgtg cggctgccca gcaatcccat
     1141 gatcatcgtc ggcaagaatg cagtgccggc aaaaacgctg ccggatctca tcgcctggct
     1201 gaagtcgcgg ccgcaacccg caaccgcggg caccgcgggc gcaggctccg gcgcccacgt
     1261 cgcagggctc gcgtttgaaa atgcggcggg tgtcaagctg caatacgtgc cctaccgcgg
     1321 cacggggcct gcgatgaacg atctcgtcgc cggccaaatc gacatcatgg tcgaccaact
     1381 ctcgaattcc atcaatcagg tgcgtgccgg aacaattcgc ggctatgcgg ttaccgatgc
     1441 caggcgcaac gacgaggcgc cggatattcc gaccaccgag gaagcgggcc tccccggatt
     1501 tcgcatgacg ctgtggtcag gcctgtgggc gcccaagggc acgccgaagg agatcgtggc
     1561 gaagctcaat tccgcggtgg tcgacgcgtt gagcgatccg caggtgcgca aggcgcttga
     1621 gaatctgggg ctgcaggtgc cgtctgcgga tcagttgacg ccggaggcgc tcggtgcctg
     1681 gcagaaatcc gaaattgcca aatggtggcc gatcatcaag gccgcgaacg tgaaggtcga
     1741 ctgatggggt gccaagtgtc gcgcagaacc ttatgagcct ccgatccgtc acgccctcgc
     1801 aggtgcgccg cgcgtttttg ttgcgcgatg aaatcgccct gctcgatgtg cgacacgagg
     1861 cggattttgc aaccggccac ccgctttttg cagccaacat ggcggccgaa cggattgtcc
     1921 tcgaagccga gatgcggctg ccgcgcaagg atgtgccgat tgttctctac gatgcgggcg
     1981 agggccacgt gcccaaagcg agggagacgt tggcggcgct cggctacacc aatgttcgcg
     2041 aacttcaggg cgggctgaaa gcctggcggg atgccggctt tgaactcttt caggacgtca
     2101 attcctacgc caaggccttc ggcgaactgg tcgagtctcg tcgccacacg ccttcgctgc
     2161 cggccgacga cgttgccgcg ctgattgcgt ccggcgcgaa catcgcggtc ctcgacgttc
     2221 ggcggttcga cgaatatgcg accatgaaca ttccgggttc ggtcagcgta cccggcgcgg
     2281 aactggtgct gcgcgcggga aaggccgcgc cggatccgga gacgaccatc gtcgtgaatt
     2341 gcgccggccg cacccgttcg atcatcggga cgcaatcgct gatcaatgcc ggcgttccaa
     2401 acaaggttgt ggcgctcaga aacggcacga tcggctggac tctggcaaag cacgcgctcg
     2461 agcatgatgc cgacaggcgc ggggccgtcg gcgcattcga aaacgccagc gccaacgcac
     2521 gcgatgtcgc gtatcgcgcc ggcgtccggc gtatcggcgt gggagacctc ggggtacttc
     2581 aggcgcagca aaaccgcacg ctctatcggt tcgacgttcg cgatgaggct gagtatacgg
     2641 ccggccatat cgcgggcttt cgccattacg cgggcggcca gctcgtgcag gagatcgacg
     2701 tggccgcacc ggtgcgcggc gcgcgcatcg tgctcaccga taatttgggc attcgtgccg
     2761 acatgacggc gtcctggctc gcgcaaatgg gatgggacgt ttacctgctg gaaggcggct
     2821 acgagggtcc gctggaggtc acgccgcctc tgaccatgcc gaaacccgat cctgcacacc
     2881 gctatcgccg cccctatgag gggacggatg cctcgcccgc caagatgcag gcctatcttg
     2941 attgggaatt cggcctcgtc gaacaactca ggcgcgatgg cacccacggc ttcttcgtga
     3001 tttgatgcgg cggacggcgg gagatcgaaa aatgccctac gcaatgaccg acgatggggt
     3061 gcggctctac ttcgaggaaa cgggatcagg ccgccccgtc atcttcgttc atgaattcgc
     3121 cggcgacttg cgcagcttcg agccgcagat gcggcatttt gggaagcgct atcgcgccat
     3181 tgccttcaat gcgcgcggct ttccacagtc cgacgtaccg gagcacgtct cctcctattc
     3241 gcagatacgt gcggccgacg acatcctcgc ggtgctcgag cacatcggcg agcgccaggc
     3301 gcatatcgtc ggattgtcga tgggcgggtt cgcgacgctg catttcggcc tgcgtcatcc
     3361 gcagcgggcg ctgtcgttgt gcatcggagg ctgcggttac ggcgccgaac cggacaagcg
     3421 cgagacgttc cgcgccgagg ccgatgtcat agccagggcg ctccggaccg agggtatggc
     3481 ggctttcgcc gagcgttacg ctttagggcc gacccgcgtt caataccaaa acaaggatcc
     3541 gcgcggtcac gccgaattca aggcgatgct ggcggagcat tcggcggtcg gatccgccaa
     3601 cacccagcaa ggtgtgcaaa aggagcggcc gtcgctttac acgctggtag aggagatgaa
     3661 gcgcatcacg gtgccggcat tgatcatcca cggtgatgag gattggccct gtcttcttcc
     3721 cggtgttctc atgaagcaaa gcattccctc cgcagcgctc gcggtgatgc cgaactgcgg
     3781 ccatgcgatc aatctcgagg aaccggacga gtacaacatc atcgtcggta atttcctggc
     3841 ccaggtcgac agcggccggt ggccacagcg cgatccccgt gcagtgagcg catccatcac
     3901 cggaatgaag gattagcggg gagcaacaga gaatgcgggg ccgtcgtgtg ctgatggatt
     3961 ctctggtgat gcacggggtg cgccatatct tcggcaatcc cgggaccacg gagacgccgc
     4021 tgctggacag tttgccggcc tatccgcaga tcgactatgt gatgaccctg catgagggcg
     4081 tcgcgatcgc agcgagcagc ttctatgcgc aagcgagcgg ccgcactgcg gttgccaacc
     4141 ttcacgtcgc ccccgggctc ggcaatgcga taggcgcgct gtatggcgcg ctcaaggcca
     4201 attcgcctgt cctggtgacc gcgggtcagc aggacacgcg catgcggctt gccaatcccc
     4261 tgctgggcca tgatctcgtt gccatggcag ctccggtcac gaagtggagc gtgcaggtcg
     4321 agcgggcaga tgagatcgcc cccatcctgc gacgcgcttt caaggtggca accgatcagc
     4381 cggcgggtcc cgtcttcgtc tccctgccga tcgacgcgct ggagcaggaa acgtcggtcg
     4441 agccggtcgc gccggaccga ttgtggcgtt gcacgcaacc ggatcccaaa ggcattggtg
     4501 aggtcgcttc gctgctcctg aaatcggcga gccccgtcat tatagccggc gacgacattg
     4561 cgcgatcgcg agccgagggg gaactcgtcg gacttgtgga ggcgattggt gcatcgctct
     4621 ggttcgaagg cctgcggcac cacgcgtcct ttccgacaag ccaccccaat taccgccaat
     4681 cgctcccggg tgatgcggtc caggtccgca acgcgcttca cggcgctgat ctcgtgctgc
     4741 tgttgggcgg accgtttttc gaagacatct ggtacgcagc gggcggccat ttccccgcgg
     4801 gcgcggtcgt cgtccaggtc gaggcatcgc ccgagcgcct ggcgttcaat catcatctcg
     4861 acgccggcgt cgtcggcgac ctctcaacca gccttgtctc gctgacggat gcggtgcgtt
     4921 cgcgcgcgac gccggaattc aggcttgccg cccaggctcg caaccgcgcg ctcgccgagt
     4981 tgcgtgcacg ggaaaaggag gcgtatcggg cgcggttgga gaagagctgg agccgtgagc
     5041 cgagttcgat gccgcgcgtg gccgctgaac tgcgacgtgg cctgcccgac gatgcagtga
     5101 tcgtagacga gagcatcacg gcaagcctcg acctcgcacg ttccttcgac tatcggggct
     5161 tcggcgatta tttcggggga cgcggcggcg gcatcgggca gggattggcc ggcgcgatcg
     5221 gggtcaagat cgcgatgccg gatcgcccgg tggttgctgt gtccggcgac ggttcggcga
     5281 tgtacagcat ccaggcgctg tggactgcag ctcaccacaa gcttgcgatc gttttcgtca
     5341 ttctgtcgaa ccgccaatat cgcatcctca agcacaatgt ggacgtctgg cgtcagaatt
     5401 tcgaagcggg gacgcagcat ccctatcaga acatggatct gaccggaccg gagcttgatt
     5461 tcgtgcatct ggcggccggc atgggtgtcc aaggcacaag ggttgaggcg gccgacgcca
     5521 tcgcaccggc cctggctgat gccgtcgcgg caaatcggcc ttatctcgtc gagattgcgg
     5581 tcgaagggaa acggtgacgc tcatgatgtg ttcaaggacc gagcggcgca attcgcacgt
     5641 attaggtaac tcctgtcgta acgccggtct gaagggtcgg catatccaaa tgctcgatat
     5701 tcagcatgcc attattgccg tttgacgaac tcgcacaggc gatcgcgggg cgccgctcgg
     5761 tctatggctc gataagtgga ctgcggctcg accgcgccgg gccgggcgag gcctggtcaa
     5821 gcttgccgta ccgcccggtt ttcgtcggcg acaccgagac cggctttctg cacggcggcg
     5881 tcgttaccgc catgctcgat gagagctgcg gtatggccgt gcagctcgcg ctagatggga
     5941 ctcgcgcgat tgccacgctt gacctgcgca tcgactacca gaagccggcg acgccgggcc
     6001 tcgacatcaa ggcgcattcg atttgctacc gcgtcacccg ctccatcgcc tttgtgcgcg
     6061 cgaccgccta tcaggattcc gaggaggatc ctgtggctac cgcaacggcc tgctttatgg
     6121 ttggcgccaa ccggaccaac atgctcacgg acaggccgct cgatgatggc gcgcccgggc
     6181 tcgacgcgcc ggaagatccg agcggtccgt ttgccagaag ccccttcgcg cgttgtctcg
     6241 gcatccgcat cagtgaggac ggcaccttgg tgatgccctt ctcgccgaag atcatcggta
     6301 acccgatcct gcccgccatt cacggcggca tgaccggcgc ctttctcgag accaccgcaa
     6361 tcgttggcgt gacacgcgag ctcggcgtct tggcgctacc gaagccgatc ggcctgacaa
     6421 tcaactatct gcgctccggt cgcgcgctcg ccagctacgc caacgcctcg atcgtcaagc
     6481 agggcaggcg ggtcatcgct ttcgaagcca aagcctggca ggaagatccg gccaagccca
     6541 tcgcctcggc gttcggtcac ttcatgctga gaaaggcgcc gggaggcgac gaggaataga
     6601 atcgccttga gttgctttgc ctgccggata gtccgcctag gcaaggtatg ggcgtgtcgc
     6661 gcgtaccatg aacggttgca ccgttcatgg gtaagcgtta gcaaatggtt gccgcagcgc
     6721 aacgattgtt gcataccagc aacaatcgtt caagatttcg cccccgcctt ggcccgctcg
     6781 cgcctacgcc gctttttagc cacacccctc catgaaatga tgttccgcaa gagcattggc
     6841 cgcggccctc gtgaacggag cgggaacctt tttcgggttt ccgcgcctaa cagggagatg
     6901 gctggggaaa cagggttgcg atggacgcca agaccgacat caagaacagg ctgccaagcc
     6961 gtcatgtgac tgagggcccg gcgcgcgctc cccatcgctc ctacctctac gccatggggc
     7021 tgaccaccga gcagattcat cagccttttg tcggcgttgc c
//
LOCUS       DATLJT010001786         5605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2288681,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001786 DATLJT010000000
VERSION     DATLJT010001786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288681"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VM910_40305"
     CDS             complement(<1..506)
                     /locus_tag="VM910_40305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HVI68768.1"
                     /translation="MTQAAMSPGMAALVLFGSFFFLIALRVPVAFALGLACLPPLVFE
                     QRLSPMVLFNETFKSYNSFILLAVPFFLLTANLMNIGGITDRLVHLSRTMVGNWPGSL
                     AQINVLLSVFFAGISGSSTADAASQSKIFIEAQTKEGYDLSFSIAITAVSAVLAVIIP
                     PSILMIVWG"
     gene            complement(503..985)
                     /locus_tag="VM910_40310"
     CDS             complement(503..985)
                     /locus_tag="VM910_40310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HVI68769.1"
                     /translation="MKKLIDGYCRLLTWLMVATVAILVVPVSLQIISRYTQLIPSYIW
                     TEELSRFLFIWMVMLGAMVAIKEGTHFEVDIWPELSRRANAMLRIVSNLFVLIFALVF
                     VYWGIKFVEFGWYQESELAELPMPFIFVAWPLAGVTWVIFLGEIFVDNIRILAGKTAP
                     "
     gene            complement(1176..2174)
                     /locus_tag="VM910_40315"
     CDS             complement(1176..2174)
                     /locus_tag="VM910_40315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HVI68770.1"
                     /translation="MSRTLRTALFVAVVAAVSALAPSARAQEAVTLHGAVQFNDDHAF
                     NKALLRFEQLVKQYYGKPINFVLHRNSELGLEKDYFAYMNQGISVDYGIVSPAHMSTF
                     SKAAPFIDAPFLFRDLDHWNKVLDADLLKPIADEIAQKADVMLIGYAGGGVRNIFVNR
                     PVRNLIEMKGLKVRVQGAPIWSRTFSAVGMSPTVIAYNEIYNAIQNGVIAAGENEAAG
                     VEQMKFYEVGPNLSMTEHAITIRPICFSGKTFKKLSPELQAAIIKAGKEAGTYGRQVE
                     SSEDSAKLDALEKAGKLKRIAFTDRAEMKKLVDPVMAAYAKEIGADAIFNQINTIK"
     gene            2321..3775
                     /locus_tag="VM910_40320"
     CDS             2321..3775
                     /locus_tag="VM910_40320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVI68771.1"
                     /translation="MDASTYGGEGDMSARKRDIRRLADASAPERRKWLARAAFFHQED
                     LRYLKFLIPEGARVLELGCGTGDLLAALKPSFGVGVDFSAGMIAQARQAHPDLTFLVG
                     DIEDAAFVRSLPGPFDVIVVVDTLGALDDCQAMFANLHDLCTRETRVVVGYFSHLWYP
                     ALKVAEATGMKMPQPPQNVLSRADVRALAALGDFDAVKDETRVLSPVRLFGLGRLVNR
                     FLAPLPLIGSLCLRHFTVCRSLRRSAEVRSVTVVIPARNERGNIEAAVKRIPRFVDAL
                     EIIFVEGHSRDGTWEEIDRVAAAHPDYDIKAMRQPGTGKGDAVFAGFDAARGDVVMIL
                     DADLTMPPEQLPKFWDAISSGKGEFVNGSRLIYPMEQEAMRVLNLIANKAFSLLFTWL
                     LSQRFTDTLCGTKALRRSDYARIKAARSYFGKFDPFGDFELIFGASKLGLKAVEIPIR
                     YASRTYGETQISRFRHGFMLLRMVLFAFMHIKAM"
     gene            3775..4524
                     /locus_tag="VM910_40325"
     CDS             3775..4524
                     /locus_tag="VM910_40325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVI68772.1"
                     /translation="MERAASAQLADHRGMWDRKPVLRLVYEDFYDRIAAACRSGRTIE
                     IGGGIGNLKRRLREVVATDIQSAPWLDCVADAQRLPFADACAANIVMVDVLHHLEFPL
                     VFFREAARVLRPGGRVLMVEPAITWGSTAFYRLFHHEPVRTSADVLEDGSPDPRRDPY
                     DSNQAIPTLLATRERDRFHRLCPTLRVAAVQWFALAAYPLSGGFQPWSLIGEGVARRL
                     IRIERTLEPVLGRFTAFRMLLTIEKAAAGGR"
     gene            4708..4893
                     /locus_tag="VM910_40330"
     CDS             4708..4893
                     /locus_tag="VM910_40330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68773.1"
                     /translation="MESEHRDLVTKTRALIETTRREISQLREEIDSARTTVDRAQRLL
                     LRTEPSTNRIARPAKAL"
     gene            4978..>5605
                     /locus_tag="VM910_40335"
     CDS             4978..>5605
                     /locus_tag="VM910_40335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HVI68774.1"
                     /translation="MDRPFPIVGVGASAGGVEALEYLFKAMPPEPGMAFVIVTHLAPK
                     RESMLPEILARDTHMPVLIAENDQIVRPNRVYVAPADAVLDITKGRLGVRAVGDGRER
                     TPIDSFFAALAEDQGEYAIGIVLSGAGSDGTLGIKAIKEHGGLTLAQATDHSGPRHSS
                     MPESAIASGLVDLAVPVETMPAQLAGYVRSFDILDKEVDKDDGAETTRN"
BASE COUNT          997 a         1811 c         1771 g         1026 t
ORIGIN      
        1 ccccagacga tcatcaaaat cgagggcgga atgatcacgg cgagcaccgc cgacaccgcc
       61 gtgatcgcga tcgagaatga caggtcgtag ccttccttgg tctgcgcctc gatgaagatt
      121 ttgctctggc ttgcggcgtc ggcggtcgaa gagcccgaga tgccggcgaa gaacaccgac
      181 agcagcacgt tgatctgcgc gagcgatccc ggccagttgc cgaccatcgt gcgcgagagg
      241 tgtacgagcc gatcggtgat gccgccgatg ttcatcaggt tggcggtcaa caggaagaac
      301 ggcaccgcca gcaggatgaa ggagttgtag gacttgaagg tttcgttgaa cagcaccatc
      361 ggggagagcc gctgctcgaa caccagcggc ggcagacacg caagcccgag cgcaaacgcc
      421 accggcaccc gcagcgcgat caggaagaag aacgagccga acagcaccag cgcggccatg
      481 cccggcgaca tcgcggcctg cgtcatggcg cggtcttccc ggccaggatg cggatattgt
      541 cgacgaagat ctcgcccagg aagatcaccc aggtcacgcc ggcgagcggc caggcgacga
      601 aaatgaacgg catgggtagt tcggcgagct cggattcctg ataccagccg aactccacga
      661 acttgatgcc ccaatagacg aacaccagcg cgaagatcag cacgaacagg ttcgagacga
      721 tcctgagcat cgcgttggcg cgccgggaga gctcgggcca gatgtcgacc tcgaaatgcg
      781 tgccttcctt gatggcgacc atcgcgccga gcatgaccat ccagatgaag agaaagcgcg
      841 acagctcctc ggtccagatg tagctcggaa tcaactgcgt atagcgcgag atgatttgca
      901 gcgagactgg aacgaccaag atcgcgacgg tcgccaccat cagccaggtc agcagtcggc
      961 aatagccgtc gatcagcttt ttcatgagcg cgagcttata ttcgagtccg acgtatcagc
     1021 tcatattgtc ttccccgcga aagcggggac ccataaccac cgcctctgga atatgggtcc
     1081 ccagcataag cgcgtttacg cgcgtcttcg acgcgctatg cgcggggacg acagacaatc
     1141 agcaggattg cgtactagcc gccgctgaag gacagctatt tgatggtgtt gatctggttg
     1201 aagatcgcgt ccgccccgat ttccttggcg taagccgcca tcaccgggtc gaccagcttc
     1261 ttcatctcgg cgcggtccgt gaaagcgatg cgcttgagct tgccagcctt ctccagcgcg
     1321 tcgagcttgg cgctgtcttc ggaggactcg acctgtcggc cataagtgcc ggcttccttg
     1381 ccggccttga tgatggccgc ttgcagctcg ggcgacagct tcttgaaggt tttgcccgag
     1441 aagcagatcg gccggatcgt gatcgcgtgc tccgtcatcg agagattggg accgacctcg
     1501 tagaacttca tctgctcgac gccggcggcc tcgttctcgc ccgccgcgat cacgccgttt
     1561 tggatggcgt tgtagatctc gttgtaggcg atcacggtcg gcgacatgcc gacggctgag
     1621 aaggtgcggc tccagatcgg cgccccttgc acgcgcacct tcaacccctt catctcgatg
     1681 aggttgcgca ccggccggtt gacgaagatg ttgcgcacac cgccgccggc gtagccgatc
     1741 agcatcacat ccgccttctg cgcgatctcg tcggcgatcg gcttgagcaa gtcggcgtcg
     1801 agcaccttgt tccagtggtc gaggtcgcgg aacaggaacg gcgcgtcgat gaagggggcg
     1861 gccttggaga aggtcgacat gtgcgccggc gacacgatgc cgtagtcgac cgagatgccc
     1921 tgattcatgt aggcgaagta gtctttctcc agtccgagtt cgctgttgcg atggagcacg
     1981 aagttgatcg gcttgccgta atattgtttg accagctgct cgaacctgag cagcgccttg
     2041 ttgaaggcgt gatcgtcgtt gaactgaacc gcgccgtgca gcgtcaccgc ctcctgagcg
     2101 cgcgccgatg gcgcaagggc ggaaacggcg gcgacgacag cgacgaataa agccgtcctc
     2161 agcgtgcgag acatggcatc ctcccggatc gtgcattttt cgttggttgg ccgatagtac
     2221 gccggcattg gtccttgtcc atgggatgga gccgaccctg cgatgagcgc cggcgccgta
     2281 atttgggcaa atggacgcta aatcgcgtat acgggtgggg atggacgcga gcacatacgg
     2341 cggcgagggc gacatgtcgg cgcgcaagcg cgacatccgc cgcctcgccg acgcgagcgc
     2401 gcccgagcgc aggaaatggc tcgcgcgcgc cgccttcttc caccaggagg atctgcgcta
     2461 cctcaagttc ctcattcccg aaggcgcccg cgtgctcgag ctcggatgcg gcaccggcga
     2521 cctcctcgcc gcgctcaaac cctcgttcgg cgtcggcgtt gatttcagcg ccggtatgat
     2581 cgcgcaggcg cgccaggcgc atcccgatct cacgtttttg gtcggcgaca tcgaggacgc
     2641 cgctttcgtg cgctcgttgc ccggcccgtt cgacgtcatc gtggtggtcg acacgctcgg
     2701 cgcgctcgac gattgccagg cgatgttcgc caacctgcac gatttgtgca cgcgcgagac
     2761 ccgcgtcgtg gtcggctact tttcgcactt gtggtatccg gcgctgaaag tggccgaggc
     2821 gacgggcatg aaaatgccgc agcctccgca aaacgtcctc tcccgcgccg acgtgcgcgc
     2881 gctcgcggcg cttggcgact tcgacgcggt caaggacgaa acgcgcgtgc tctctccggt
     2941 gcggctgttc gggctggggc gcctggtcaa ccgcttcctg gcgccgttgc cgctcatcgg
     3001 cagcctgtgc ctgcgccatt tcaccgtttg ccgctcgctg cgacgatccg ccgaggtgag
     3061 atccgtgacg gtcgtcattc ccgcgcgcaa cgagcgcggc aacatcgagg cggcggtcaa
     3121 gcgcatcccc cgcttcgtgg acgcgctcga aatcatcttc gtcgaggggc actcccggga
     3181 cggcacctgg gaggagatcg atcgcgtcgc cgccgctcat cccgattacg acatcaaggc
     3241 gatgcgccag ccggggacgg gcaagggcga tgccgtgttt gccggcttcg acgctgcgcg
     3301 cggcgacgtc gtgatgatcc tcgatgccga tctgaccatg ccgccggagc aattgccgaa
     3361 gttctgggac gcgatttctt ccggcaaggg cgagttcgtc aacggctccc gtctcattta
     3421 tccgatggag caggaggcga tgcgcgtgct caacctcatc gccaacaagg cgttctcttt
     3481 gcttttcacc tggctgttgt cgcagcgctt caccgacacg ctgtgcggca ccaaggcgct
     3541 gcggcgctcc gactatgcgc ggatcaaggc cgcgcgcagt tatttcggca agttcgatcc
     3601 attcggggat tttgagctga tcttcggcgc ttccaaactc ggcctcaagg cggtcgagat
     3661 ccccatccgc tatgccagcc gcacctacgg cgagacccag atctcccgtt tccgtcacgg
     3721 cttcatgctg ctgcgcatgg tgttgttcgc gttcatgcac atcaaggcga tgtgatggag
     3781 cgcgcggcat ccgctcagct cgcggatcac cgcggcatgt gggatcgcaa gccggtcctg
     3841 cgcctcgtct acgaggattt ttacgaccgc atcgccgcgg cctgccgaag cggccgcacc
     3901 atcgaaatcg gcggcggcat cggcaacctc aagcgccggc tgcgcgaggt ggtcgcaacc
     3961 gatatccaat ccgcgccgtg gctcgactgc gtcgcggatg cccagcggct gccgttcgcg
     4021 gatgcctgcg ccgccaatat cgtcatggtc gacgtgctgc atcatctcga atttccgctc
     4081 gtgttcttcc gcgaggccgc gcgggtgcta cggccaggcg ggcgcgtgct catggtggag
     4141 ccggcgatca cctggggcag caccgccttc taccggctct ttcatcacga gcccgtgcgc
     4201 acctcagccg acgtgctcga ggacggcagc ccggacccgc gccgcgaccc ttacgactcc
     4261 aaccaggcga tcccgacgct cctcgccacc cgcgagcgcg accgctttca tcgcctctgt
     4321 cccacgctgc gagtcgccgc cgtgcaatgg ttcgcgctcg cggcctatcc gctgagcggc
     4381 ggcttccaac cctggagcct gatcggcgaa ggcgttgcgc ggcgcctcat acgcatcgaa
     4441 cgcacgctcg agcccgtgct cggtcgcttc acggcgtttc gaatgctgct gacgatcgag
     4501 aaggcggcgg ccggcgggcg gtgagttttc gtcagccggc aagtcgattt cgcccgcgag
     4561 cgcaatggct ttgctggtac gcggccaaag catccggaac caaataccct ttcgtcggtt
     4621 gtacttaacc ggccgactcc gtcgtggagg cgcgtgccgg gacatgtcgt ggctgccgac
     4681 gattgaaagc cctcgggagc gaatcccatg gagtccgagc atcgcgacct cgtgaccaag
     4741 acgcgggcat tgatcgagac cacccgccgc gagatttcgc aactgcgcga agagatcgac
     4801 tcggcacgga ctacggtcga tcgcgcgcag aggcttttgt tacgtaccga accatccacc
     4861 aatcgcattg caagacccgc aaaggctctt tgaattcgca gggccttggc gtagccttgg
     4921 cgcgatccgc ccaccgcagg cagacccgtt tcctctcgat ccctgctggg ggagccgatg
     4981 gataggccat ttcccattgt cggcgtcggc gcttccgcgg gaggcgtcga agcgctcgaa
     5041 tatctgttca aggccatgcc gcccgagccc ggcatggcct ttgtcatcgt cacccacctc
     5101 gcccccaagc gggaaagcat gctgccggaa attctggcgc gcgatacgca catgccggtg
     5161 ctgatcgcgg agaacgacca gatcgtccgc cccaatcgcg tctatgtggc gccggccgat
     5221 gcggtgctcg acatcacgaa ggggcggctg ggcgtccgcg ccgtcggcga cggccgcgag
     5281 cgcaccccga tcgactcgtt cttcgccgcg ctggccgagg accagggcga gtatgccatc
     5341 ggtatcgtgc tctccggcgc cggcagcgac ggcacgctcg gcatcaaggc gatcaaggag
     5401 cacggcggct tgacgctggc gcaggcgacc gaccactccg gcccgcgtca ttccagcatg
     5461 cccgaaagcg ccatcgcgtc cgggctggtc gacctggcgg tgccggtcga aaccatgccc
     5521 gcacagcttg ccggctatgt gcgcagcttc gacatcctcg acaaggaggt ggacaaggac
     5581 gatggggcgg agactacgcg caatg
//
LOCUS       DATLJT010001787         1634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_4359223,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001787 DATLJT010000000
VERSION     DATLJT010001787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4359223"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(727..1128)
                     /locus_tag="VM910_40340"
     CDS             complement(727..1128)
                     /locus_tag="VM910_40340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVI68775.1"
                     /translation="MRLAGHYVAAGYAVLVPERRGYGRSDGPSWSEAVGRDIGVVLIA
                     RLQAEADDVLAAVEYLRGVPSADISRLGIAGWSLGGIVTLFAISRSRSFRAAIDQAGG
                     VLMWRFSPALQAALTQAARAMTCPVLLMRAE"
     gene            complement(1412..>1634)
                     /locus_tag="VM910_40345"
     CDS             complement(1412..>1634)
                     /locus_tag="VM910_40345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68776.1"
                     /translation="TSQRLTEITLEETLRGSVFIGSPDTVVGKLKDLQRDIGLSGVQL
                     ETNCAGLADHAAEREAIRLLAREVKSAFR"
BASE COUNT          333 a          515 c          534 g          252 t
ORIGIN      
        1 ggaagcccca ctgcgtgatc agggatgatc aaccggaggc ccgtgaggga caggccgggc
       61 gcactggggt ggcggagagg cttgtagtac cgatgaagcc gggtaatgcc ggggagggaa
      121 ggggcctcag ttcaagacaa acgcgacaag cggtgaggga ccgggagatt gggcaaccta
      181 gcaactccga aaagtgttca gacactgcag acggcgttac acgcgaaaac gcgtgaagtc
      241 ttgtccgaga gccggatgcg ggaaatctgc atgtccggtt cgatgagcgg agtgtggaaa
      301 cggagccagg gtcgaaccag tgaggcaccg tcagacgaaa ggggcggcaa cagatatgtt
      361 cggcctacag ccaccgcgcc acactccgac tctaccgaaa ttgcccgttc aggaacgagg
      421 gcctaaggtc cggaatcggc gaatgagcca tcaagttgcc gacatacctc acgcgtctcg
      481 gccgctttgg ggccggctcc tctcggcagg atcaattcgt gcagctatcg ccggaggtag
      541 gcgtcaaaaa agcctatcgc gtcgcgcccc cagatcggca aaccttcggc cgagaacaag
      601 agatggccgg gtgcgaccgc cgcctcctgt gcggtgggag taaacggcgg atacatgatc
      661 agccggtgcg gcaacccggc ggcgtccatc acctatgaga gtgtcggtat cgcctcgggg
      721 gcggcgtcat tctgcgcgca tcaacaacac tgggcatgtc atagcgcggg ccgcctgcgt
      781 cagtgctgcc tgcagtgccg ggctgaagcg ccacatcaat acgccgccgg cttgatcgat
      841 cgcggcccga aagcttcggc tgcgcgaaat cgcaaagagc gtgacgatgc cgccgagcga
      901 ccagccggcg atccccaaac ggcttatatc ggcggatggc acgccgcgca ggtattcgac
      961 ggcagcgagt acgtcgtcgg cctcagcctg caaccgcgcg atcaatacga cgccaatatc
     1021 gcggccaaca gcctcgctcc aggacggccc gtcggagcgg ccgtagccgc gccgctccgg
     1081 gaccagcacg gcataaccgg cagcgacata gtggccagcg agccgcactc acgggttggg
     1141 ccgccgctca aacccggcgc gcgacccgtg gttgtagata agggtcggga aagggccgga
     1201 cccgtcgggc tgatagaaat agccctgaat gcgtagcccg ccgctctgat agttgacctc
     1261 ggtgtaaggc tgctgcgcgc gcgtcgcgca cggtgccagc agtggcacaa gcccggcggt
     1321 tcaccggccg ttcagcccgg gcggcgggaa cgggtcaatg tgccccactc cggccattca
     1381 cgatcgtcgc cgcggaggct cgaagccgcg ctcaacggaa cgccgacttg acctcccgcg
     1441 ccaatagccg gatcgcctct cgctccgccg cgtgatcggc caggcccgcg caattcgttt
     1501 ccaactgcac gcccgacagc ccgatgtcgc gttgtaggtc tttcaacttc cccaccaccg
     1561 tatcgggtga gccaatgaac accgaaccgc gcaatgtttc ctcgagcgtg atttcagtga
     1621 ggcgctgcga ggtt
//
LOCUS       DATLJT010001788         1572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_911401,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001788 DATLJT010000000
VERSION     DATLJT010001788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911401"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VM910_40350"
     CDS             <1..947
                     /locus_tag="VM910_40350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVI68777.1"
                     /translation="VFTLPAEIAAIAYQNKNAIYALLFKAASDTMLTIAADPKHLGAK
                     IGITAVLHTWGSAMTHHPHVHMIVTGGGISPDGSRWIASRPDYLVPVEVLSALFRGRM
                     LGMLIDAHAAGRLKFFGAYQHLADTLAFRAYLGPLWTTDWFVYAKRPFAGPEQVLAYL
                     SRYTHRVAISNSRLISADATGVTFKYKDYRIEGPDRYTAMTLEPGEFIRRFLMHVLPK
                     GFHRIRHYGLLANGGLTRAEKIARARQLIAAAPQITPSPQPPQHDPTYEATTLSEELD
                     HPCPHCGSRMVIIETFQPGCRPRHRPSAPVSTIGINTS"
     gene            complement(1449..>1572)
                     /locus_tag="VM910_40355"
     CDS             complement(1449..>1572)
                     /locus_tag="VM910_40355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68778.1"
                     /translation="ADLPVQAPSKYALTVNLKTAKALGLAVPSALLARADEVIE"
BASE COUNT          285 a          584 c          417 g          286 t
ORIGIN      
        1 tcgtgttcac gctgcccgcc gagatcgcag ccatcgccta ccagaacaaa aacgcgatct
       61 acgccctcct gttcaaggcc gcttcggaca ccatgctcac cattgccgcc gaccccaagc
      121 acttgggcgc caagatcggc atcaccgccg tcctgcacac ctggggttcg gcgatgaccc
      181 accatcccca cgtgcacatg atcgtcacgg gcggtggcat ctcgccggat ggatcacgct
      241 ggatcgcctc acggccggac tatctcgtcc ccgtcgaagt gctctcggcc ctattccggg
      301 ggcgcatgct ggggatgctg atcgacgcgc atgccgccgg ccgcctcaag ttcttcggcg
      361 cttaccagca tcttgccgac acgctggcct tccgagccta tctcggcccg ctgtggacga
      421 cggactggtt cgtctatgcc aagcgtccgt tcgcgggccc cgagcaggtc ctggcctacc
      481 tgtcccgcta cacccatcgt gtcgctattt ccaacagccg cttgatctcc gccgatgcga
      541 cgggtgtgac cttcaaatac aaagattacc ggatcgaggg ccccgatcga tacacggcca
      601 tgacgctcga gcccggcgag ttcattcgcc ggttcctcat gcatgtgttg cccaagggtt
      661 tccaccgcat caggcactat ggcctgctcg ccaatggcgg cctcacccgc gccgagaaga
      721 tcgcccgcgc tcgccagctc atcgccgcag caccccaaat cactccgtcg ccccagccac
      781 cacagcatga cccaacctac gaggcgacga ccctttcaga ggagctcgac catccctgcc
      841 cgcattgcgg ctcgcgcatg gtgatcatcg agacgttcca gccgggttgc cgccctcgcc
      901 accgtccctc cgcccccgtg tcgacaatcg gtatcaacac gtcatgacgc tgcgcgccat
      961 cacctcgatc accgtcttct tctctctgct gctgccttgt gccagccacg atagggcacg
     1021 gccccttcgg agctcctgcc ggcgatctac cacgcaatca gccgatcatc cgctgaaaat
     1081 cacgccacaa ctccaccttg cggctgcgcc tcgcctcaac aatcagcgcc gacgcttgtc
     1141 tcacagcacc cggctgcgag ggccgcgcga gtcaaatccc catagcgctc gctgataggg
     1201 ctgccacctc ccgcggtttc gttcactggc ggctttcgaa cgccggcccc cggccttggc
     1261 gcatgtcgcg agcttggcga gcgggccggc atccgaaacc cgtcacacga gcacgcatcc
     1321 cgggtgcctg gccgcgacgg tgcaagtgcc tcaataacca gacatgatcg ccgcagcgca
     1381 gcgaactggt aaagtgtgcc agaatccgga cctgagcatg cgcaacatac atagcgaccg
     1441 aagagcccct attcgatcac ctcatcagcg cgggcgagta gcgccgaggg gaccgcaaga
     1501 ccaagtgcct tcgcggtctt tagatttact gtcaacgcgt atttactcgg cgcttgcacc
     1561 ggcagatcgg cc
//
LOCUS       DATLJT010001789         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1376459,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001789 DATLJT010000000
VERSION     DATLJT010001789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376459"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VM910_40360"
     CDS             complement(<1..795)
                     /locus_tag="VM910_40360"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVI68779.1"
                     /translation="MSASSAKADRAFVRLTNVGKSFRIESGAIEVLRNVSIDVADRQF
                     ISILGPSGCGKSTLLMMLAGLEPVSSGAIEIAGQAMRAPRRDVGVIFQDPTLLPWKSA
                     IENVLFPIEIFGLPFAKYQARAEALLELVGIPEARHRRPRQLSGGMRQRVAICRALIH
                     EPKLLLMDEPFSALDAITRDELNVTLLDIWERYHQTAFFVTHSIREAVFLSDRVVVLG
                     GWPGQVVLDVTIPFARPRDLSIGESGDFNQICGELRRAIAENHARRV"
     gene            complement(808..1917)
                     /locus_tag="VM910_40365"
     CDS             complement(808..1917)
                     /locus_tag="VM910_40365"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68780.1"
                     /translation="MDTNCSTRISRRQALAGTAAALAGFKFPTPAIAQGATSIKMTLP
                     WLPQGSQFFAFVARNRGYWKTRGLNVEIARGFGSAAAIQTITQGQMQTGIIAAPSVLV
                     GAAQDLDTRVVGIAGYDSTMGFLVLDESPVKSLKDLEGRKLGSTPQSAEVPFIDHFLT
                     LSGVDPSKVSRVALQATALETTLMNKQVDAISVFATSNMPSLLVQGVKFRFYNFADVG
                     IRLYSNCLTTSPTYLNDNKSVVEAWADGMNEGLKYSVTNFEDAVDIFVSEVPEVKMSS
                     TGKAHTRYGAGLFLAAYLTPELREHGIGWGDPVSLKNQAELVMKYAVGQGAKRPDTAV
                     IFSNAMAGKIKLTASEWELARKSASEFAPLLGIKL"
     gene            complement(1941..>2902)
                     /locus_tag="VM910_40370"
     CDS             complement(1941..>2902)
                     /locus_tag="VM910_40370"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVI68781.1"
                     /translation="LAIGTMTFGSQMDEKSAHRLLDEAYAAGVNFFDTAEVYPAPAQA
                     STYGLAELILGRWLKRLPRDQIIISSKVAGGQRPEGPHLPWVRGGATAVDVAQVCAAC
                     DGSLQRLGTDYIDIYQPHWPDRKVPIEVQLEALMRLVEQGKVRRIGLSNETAWGVTVF
                     CAAARHQSRPVCVQNAYNLLQRRIEHGLVEACEREQVSLIAYSPLAMGVLAGKHSGGC
                     RPDRARLSSPNRYSEMYLQEQLLSVADAYVEVAQAHGLDPAVMAYAWVRQQPMVASVL
                     SSFSRSEQLEPFVRSAQIRLDPGILSQLDAVRQTHDARWNILG"
BASE COUNT          562 a          896 c          866 g          578 t
ORIGIN      
        1 gacgcggcgg gcgtgattct cggcgatcgc tctgcgtagt tcaccgcaga tctgattgaa
       61 gtcgccgctc tcgccgatac tcaagtcgcg tgggcgcgcg aatgggattg taacatccag
      121 caccacttgc ccaggccagc cgccgagtac gacgactcgg tcagacaaga agaccgcttc
      181 acgaatggag tgggtcacga agaacgccgt ctggtggtag cgctcccaga tatcgagcag
      241 agtcacattg agttcgtccc gcgtgatcgc gtcgagggcg gaaaacggct cgtccatcag
      301 caacagcttc ggttcgtgaa tgagcgcccg gcagatcgcc acccgttggc gcattccgcc
      361 cgagagctga cgcggtcgtc gatggcgcgc ttccgggatc ccgacaagct cgagcagcgc
      421 ctcggcgcgc gcctggtact tggcgaaggg aaggccgaag atttcgatcg gaaacaggac
      481 gttttcgatc gccgatttcc acggcaacag cgtcggatcc tgaaaaatca cccccacgtc
      541 gcggcgcgga gcgcgcatgg cttgaccggc aatctcgatt gcgccgctgc tgaccggctc
      601 gagcccggcc agcatcatca acagcgtgct cttgccacag ccggaaggac cgaggatcga
      661 aatgaactgc cgatcggcca cgtcaatgct gacgttgcgc aatacttcaa tcgcgccgct
      721 ttcgattcga aagctcttgc cgacgttggt cagtcgcaca aacgcacggt cagctttcgc
      781 gctactggcg gacatacgag ggtccgttta gagcttgatc ccaagcagcg gcgcaaattc
      841 gctcgcggat ttgcgcgcca gctcccattc ggaagcggtg agtttgatct tgccggccat
      901 cgcgttgctg aaaatgacgg cggtgtcagg ccgtttcgca ccttggccga cggcatattt
      961 catcaccagc tccgcctgat ttttgagcga gactggatcg ccccaaccaa ttccgtgctc
     1021 gcggagttcg ggcgtgagat aggcggcgag gaatagcccg gcgccgtaac gggtatgcgc
     1081 cttgcccgtc gacgacattt tcacctccgg gacttcgctg acgaaaatat cgaccgcgtc
     1141 ctcgaaattg gtgacgctgt acttcagtcc ttcgttcatg ccgtcggccc atgcctcgac
     1201 cacggactta ttgtcgttga ggtaggttgg gctcgtcgta aggcagttgg agtacaggcg
     1261 gattcccacg tccgcgaaat tatagaatcg gaatttcacc ccctgcacga gcaggctcgg
     1321 catgtttgag gtcgcaaaga ctgaaatggc atcgacttgt ttgttcatga gtgtggtttc
     1381 gagcgcggtc gcctgcaatg ccacgcggct gactttgctg ggatcgaccc ccgacagggt
     1441 caggaaatgg tcgatgaacg gcacttccgc cgattgcggc gtggatccca acttgcgccc
     1501 ttcaagatcc ttgagcgatt ttaccggaga ctcgtcgagc accaggaaac ccatcgtgga
     1561 gtcgtagccg gcgatgccga ctacgcgagt gtccagatct tgcgcggccc cgacgagaac
     1621 ggatggggca gcgatgatac cggtttgcat ctggccttgc gtgatcgtct ggatggcggc
     1681 ggccgagcca aagccgcggg ctatctccac attcagtccg cgcgttttcc aatagccacg
     1741 attccgcgcg acgaaagcga agaactgcga gccctgtggc aaccacggca gagtcatctt
     1801 gatgctggtt gcaccttgcg cgatcgcggg tgttgggaat ttgaaacccg ccaacgccgc
     1861 tgcggttccg gccagcgcct gccgtcgtga tattcgcgtg gagcagttcg tgtccatcac
     1921 acactccgtt tagtcagtgt tcatccaagg atattccagc gcgcgtcgtg cgtctgcctc
     1981 acggcgtcga gctgcgacaa gattcctggg tcgagccgaa tctgtgcgct gcgcacaaat
     2041 ggttcgagct gctcgctccg cgagaaactg gagaggacgc tcgccaccat cggctgctgg
     2101 cgcacccagg cgtacgccat gacggcgggg tcgagaccat gcgcctgggc gacttccacg
     2161 tacgcgtccg caacgctaag tagctgctct tgaagataca tctcgctgta gcgattggga
     2221 ctggatagtc gtgcccgatc cgggcggcat ccgccggaat gctttccggc cagcacgccc
     2281 atcgccaacg gcgaataggc gatcaagctc acctgttcac gctcgcaggc ttcgaccaaa
     2341 ccatgctcga tcctgcgctg caaaaggttg tatgcgttct gcacgcacac cggacgcgat
     2401 tgatggcgcg cggctgcgca aaaaacggtc acaccccacg ccgtctcgtt gctcaggcca
     2461 attcggcgca cctttccttg ctcgaccaga cgcatcaggg cttcgagctg cacctcgatg
     2521 ggcaccttcc gatcgggcca atgcggctga tagatgtcga tgtaatcggt tccgagccgc
     2581 tgtaacgagc cgtcgcacgc cgcgcacacc tgggcaacgt caacggcggt cgcgccgccc
     2641 cgcacccacg gcaaatgcgg gccttcgggc cgctgtccac ccgcaacctt gctggagata
     2701 ataatctggt cgcgcggcaa acgcttcaac cagcgtccca atatgagctc cgcgagccca
     2761 taggtgctcg cttgtgctgg cgccgggtag acttcggcgg tatcaaagaa attcacgccc
     2821 gccgcatacg cttcatcgag caggcggtgg gccgatttct catccatctg gctgccgaag
     2881 gtcatcgtgc cgattgcgag at
//
LOCUS       DATLJT010001790         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_1143120,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001790 DATLJT010000000
VERSION     DATLJT010001790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143120"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..704)
                     /locus_tag="VM910_40375"
                     /pseudo
     CDS             complement(316..704)
                     /locus_tag="VM910_40375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648492.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
     gene            940..1071
                     /locus_tag="VM910_40380"
     CDS             940..1071
                     /locus_tag="VM910_40380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68782.1"
                     /translation="MYHGVPMRDLVADWKKWSRAERVIAVMGTLLMVALPLGLLMTG"
     gene            complement(1158..1400)
                     /locus_tag="VM910_40385"
     CDS             complement(1158..1400)
                     /locus_tag="VM910_40385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68783.1"
                     /translation="MARAVAKAAHAPRPGIPVLYVGVCHGPGTRAPPTSGNIDYFQGY
                     KRAGDILIQNALADLYDRDSLIFPAIFNYRHLLSCI"
     gene            complement(1530..1733)
                     /locus_tag="VM910_40390"
     CDS             complement(1530..1733)
                     /locus_tag="VM910_40390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68784.1"
                     /translation="MPDEVEIGQLVHEIEMIEARLIAARHLRLTDRVRLLEDQKRRLE
                     ARLAELQAEPGDAPPKTPSLMRI"
     gene            complement(2274..>2594)
                     /locus_tag="VM910_40395"
     CDS             complement(2274..>2594)
                     /locus_tag="VM910_40395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68785.1"
                     /translation="YNEYMPPERREDPIVQDIHLLSRCAARPEIVWCQHHNGVFRSED
                     AGANWQEQTAIRPSKFGFAVAAHLRDPDTAWFVPAIKDVRRIPVDGKVVVARTRDGGR
                     SFEV"
BASE COUNT          474 a          812 c          801 g          507 t
ORIGIN      
        1 ccgtggatag ggcaagcagc aatggtcctg ctgctacgag cacgcgcaga tctcgcttca
       61 tttccgagta gcctcctgtt ctgtcacgcg ggctcacaaa gcccgccgcc ccatccttgc
      121 cggcccgttt gccgagcttt cttgcaggat gacaacgctc aataggctat cacctatcga
      181 agacaagaaa tagcgccggt atcggtatca cctccgccgt cccggcgcgg ccgtgccgcg
      241 acgggttgct tgtcgagcca gacgtcttcc atgcgccagc cgttgcagat gccgttgacc
      301 atcaccgtcc accccttgac aggccacgtg atagcggggc tcgcggccgg tacggccggt
      361 gaagagtgcc cgcgggatat tgaccatgtg cggcgagcgc ttgcgctcgt agagcagcgg
      421 cgtgccgcac cgcgagcaga agcttcgggt cgactccgtc gtcgcatcct cgaagcgagc
      481 gatgcttcgt cggcccttcg ccacgcgggc gtgcttcgcc aacagccgat ataggtcgca
      541 tacgcggcac cgtgcgcgcg acggcttgcc gtggagtgat cgtgccaggc ccaaaaagcg
      601 ggaaagtcga tctctagctt cactgcgcca cagaggcaac ggccagacac ttgccggccc
      661 gataggtttg gcgcggcgcg tttattcagg ctctccaatt tcatgctcga ccccgcaatg
      721 acggatgggt ttccatacgg gagagacgat cgaaaataat catagcagcc ggcaataaaa
      781 gcccgggcgg cttcctcttc gacggctgtc gcgatcgaaa tcccccgata acgaaccgct
      841 cgatcgaagc aggtctcact tcgccggtag tgaaagcggg tatatctgcc agtcgtagcg
      901 ctcgccctgc cttcggggaa ggcagtcgcg agcgaatcca tgtaccatgg cgtgccgatg
      961 cgtgatctcg ttgccgactg gaagaaatgg agccgtgcgg agcgcgtaat cgcagtgatg
     1021 ggtactttac tgatggttgc attgccgctc ggattgctga tgaccggcta gatcggcccg
     1081 gagcgccggg accttccggg gcatcgcgtc ttgcaggagc gccttcgcct gagcggtcgg
     1141 caggcgatgc ttgcggctca aatgcaggag agcaaatgcc gatagttgaa aatggccggg
     1201 aagatcaggc tatcccggtc gtacagatcg gccagggcgt tctggattag gatgtcgccc
     1261 gcccgcttgt aaccttgaaa gtagtcgata tttccgctcg tcggcggcgc ccgcgttcct
     1321 gggccgtggc acaccccgac gtagagcacc gggatgcccg gtcgcggagc gtgagcggct
     1381 ttggcgacgg ctcgagccac tttccgtcga ccacatgccg ctctccgagc cgccgccgcg
     1441 ggagagccgc tttgggtcgc cgctgaaatt gtcgaaggtt cattgggtgc ggccggagat
     1501 ccggtgcttt gcggggtcgc tgttcgctgt caaatccgca tgagagaggg cgtctttggc
     1561 ggggcgtcac ccggctccgc ctgcagctcg gcaaggcgcg cctcaagcct tcgcttctga
     1621 tcctcgagca agcggacgcg gtcggtgagc ctcagatgcc tcgccgcaat gagccgcgcc
     1681 tcgatcattt cgatctcgtg gaccaattgt ccgatttcga cttcatccgg cattggctat
     1741 ccgtcctttg gtcgcaacaa gcacaagatc tgtcagatcg aatttaatca gccgatcagc
     1801 ggtccactct ctgcgcgcga gatccctgac ccggatcgcg caagcaaacg aggcgcttgg
     1861 ctacgactat cccattcaat ccaagcgtat cggtccgaaa gcgagacgat tcatgccgtg
     1921 ccttttgcaa aggagctgac atgctggtcc aaccgtacct gtcctttgac ggacgctgcg
     1981 aagaggcgat cgagttctgc cgtaaggcgc tcggtgccgc gacttgccca attaaggcat
     2041 ccggctccta ccttgggtgt ctgacggtct gttcaatgta gagccggggc gaagcgcacc
     2101 gtcgcgatcg gcggcagccg cgcctcgggc atcgtccagg aatccccgcc attctccgag
     2161 atccacaacc cgccgctggt cgaaccaaag gcgaggagct caccggtctc gtcgaccgcg
     2221 agcgcatgtc gccacgccag atcataggca tggcgttgtc gtaggccgtt cttttacacc
     2281 tcgaagctcc ggccgccgtc tcgggtgcgc gcgacgacca ccttgccatc gaccgggatc
     2341 cgccgcacgt ctttgatcgc ggggacaaac caggcggtat ccgggtcacg caaatgggcg
     2401 gcgacggcaa agccgaattt cgatgggcgg atcgctgtct gctcctgcca attagccccc
     2461 gcgtcttctg agcggaacac gccattgtgg tgctggcacc agacgatttc ggggcgcgcg
     2521 gcgcagcgcg acagaagatg gatgtcctgg acaatcggat cctcgcgccg ctccggcggc
     2581 atatactcgt tgta
//
LOCUS       DATLJT010001791         5355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_455296,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001791 DATLJT010000000
VERSION     DATLJT010001791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455296"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..222)
                     /locus_tag="VM910_40400"
     tRNA            complement(146..222)
                     /locus_tag="VM910_40400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Met,seq:cat)
     gene            520..1953
                     /locus_tag="VM910_40405"
     CDS             520..1953
                     /locus_tag="VM910_40405"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HVI68786.1"
                     /translation="MDQSLKSPPLSALDDAFHAMLDASRREPPPPLELRLDRLRRLRE
                     IVSDNEARFEQAISADFGHRSPTETLFAETLFVLGEIRHAAKHLKRWMAPQRIATALQ
                     FLPAKNRLIPQPLGVVGIIAPWNYPLQLTIAPAVGALAAGNRVMIKPSELTPHFAALL
                     KELIAAKFAPDEMMVTGIEEEIARAFASLPFDHLVFTGSTRIGRLVSEAAGRNLTPVT
                     LELGGKSPAIVDRSADLDEAAERIAYGKLLNAGQTCIAPDYAFVPEEKAVAFAEKVRT
                     HMRRMFGTEPGNKDYTSIISDRHYARLERLVADAAAKGAKILQPAKADDPNWKARRKF
                     PPTIVVGATSDMTVMQEEIFGPVLPVLGYRDADETIAYVNKHDRPLALYWFGRNSTAR
                     DQVLTHTVSGGVTVNDCLFHFTQINQPMGGVGASGSGAYHGEWGFKTLSKLKPVFYRS
                     VLNRLADLYPPYGGRIARLEKMLRFMS"
     gene            2061..3665
                     /locus_tag="VM910_40410"
     CDS             2061..3665
                     /locus_tag="VM910_40410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HVI68787.1"
                     /translation="MTDTFDFVVVGAGSGGCAVASRLSEDAATSVALLDAGGKCDNWV
                     VTTPGAIILMISGKLNNWAFNTVPQKGLNGRIGYQPRGKGLGGSSAINAMVYIRGHRV
                     DYDHWASLGNTGWSYADVLPCFKRSEDNAEFDGEFHGKGGPLHVSGLRSDNPVKEMFL
                     QGAREAQFRIRDDFNGAEQEGLGVYQVTQNNGERWSAARGYIHPFMGTRGNLRVETHA
                     QATRILFEGKRAVGVEYLQGKQKKQLRARREVILSAGAFQSPQLLMLSGVGDAASLAR
                     YGIASLHHLPGVGQNLQDHPDFVFGFASDAPYFSGLSWSGIGRILKGIGQYRRERRGP
                     LTSNVAECGGFLKTRPDLDLPDIQLHFCMAVVEDHGRKPRWGTGFSCHVCLLRPKSIG
                     SVWLDSADPLAPPAIDPNFLGEDSDLDTMVAGFKMTRRLLEAPTLRKLQKADMFTNGI
                     ASDEDIRKVLRARVDTVYHPVGTCKMGVNDASAVVDPRLKVYGLEGLRVVDASIMPTL
                     IGGNTNAPTIMIGEKAADMIRAEMRA"
     gene            complement(3774..4013)
                     /locus_tag="VM910_40415"
     CDS             complement(3774..4013)
                     /locus_tag="VM910_40415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68788.1"
                     /translation="MRVLVWFGFVLVATAAQAQSYTTTVNRETNTVTTTGPGYSASTT
                     QISRTATSTTYTTTITRNPGGYQPMGAGGYHPMGH"
     gene            4294..5076
                     /locus_tag="VM910_40420"
     CDS             4294..5076
                     /locus_tag="VM910_40420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVI68789.1"
                     /translation="MDLGIKGRRAIVCASSKGLGRACAIALANEGVHVTLTARGAEAL
                     KATAEEIRKKSPGVTVTEVVGDITTPAGREAVLKACPEPDILVNNAGGPPPGDFRNWT
                     RDDWIKAIDANMLTPIELIKATVDGMMARKFGRIVNITSAAVKAPIEILGLSNGARAG
                     LTGFIAGISRKTVINNVTINGLLPGPFATDRLRGTAKAEADKRGITPDQLLAERAKQN
                     PAGRFGDPEEFGLFCAFLCGAKAGFITGQNILLDGGAFPGTL"
BASE COUNT         1026 a         1669 c         1705 g          955 t
ORIGIN      
        1 aatcagttcg agcgttgcgg agatgttggc agcgatcacc tcaaactctc cgcctgacga
       61 agcgtttgga accggcggcc aactcgccgc cctgctcagg gtttccgaga ggacggccga
      121 gcccctggca aagtccaggg agcgttggtt gcgggggcaa gatttgaact tgcgaccttc
      181 aggttatgag cctgacgagc taccgggctg ctccaccccg cgataaacca ttgcgcgcct
      241 tgagagaaaa ccgctgacga aacggggtgg ggcggcttca aagcgcgccg gtccgttcgc
      301 tcataggact tcctgagaag gcaacccgga ggccaaggcc ccgtcgggtg ccggggctat
      361 gtatcaacgc cgcttccctt tggaaagggt tttgggccgc ctttttcgat tttatgacag
      421 acaaaatgag gcaaaagccg cggaaaaaca ccagcccttg ccagaagttc ggcaaaggag
      481 gagccttccc gccaacaagg tcaaaaacag gggaacgcca tggatcagtc cctgaagagc
      541 ccgccccttt cagccctgga tgacgctttc catgccatgc tggacgcctc gcggcgcgag
      601 ccgccgccgc cgctcgagct tcgcctcgac cgcctgcggc ggctgcgcga gatcgtcagc
      661 gacaacgagg cccgcttcga gcaggcgatc tctgccgatt tcggccaccg ctcgccaacg
      721 gaaaccctgt tcgccgagac gctgttcgtg ctcggcgaga tcaggcatgc ggcaaagcac
      781 ctcaagagat ggatggcccc gcagcgcatt gcgaccgcgc tgcaattcct gccggccaag
      841 aatcgcctga tcccgcagcc gctcggcgtg gtcggcatca tcgcgccctg gaattatccg
      901 ttgcagctca ccatcgcgcc ggcggtcggc gcgctcgccg ccggcaaccg ggtgatgatc
      961 aagccgagcg agctgacgcc gcatttcgcc gcgctcctca aagagctgat cgcggcaaag
     1021 ttcgcacccg acgagatgat ggtcaccggc atcgaggagg agatcgcaag agcattcgcg
     1081 tcgctaccct tcgatcatct ggtcttcacc ggctcgaccc ggatcggccg tctcgtctcg
     1141 gaagccgcag gccgcaacct cactccggtg accctggagc tcggcggcaa gtcgcctgcg
     1201 atcgtcgacc gctctgccga tctcgatgaa gccgccgagc gcatcgccta tggcaagctg
     1261 ctcaatgcgg gacagacctg catcgcgccg gattatgcct tcgttcccga agagaaagcc
     1321 gtggcttttg ccgaaaaggt gcgcacccat atgcggcgca tgttcggcac cgagcccggc
     1381 aacaaggact acacctccat catctccgac cgccattatg cgcgtctgga acgcctagtg
     1441 gcggatgccg cggccaaggg cgcaaagatc ctgcaaccgg cgaaagcgga cgatccgaac
     1501 tggaaggcgc ggcgcaaatt cccgcccacc atagtggtcg gcgccacatc cgacatgacc
     1561 gtgatgcagg aggagatttt cggacccgtg ctgccggtgc tcggctatcg cgacgcggac
     1621 gagacgatcg cctatgtcaa caagcacgac cggccgcttg cgctgtactg gttcggccgc
     1681 aacagcacgg cacgcgacca ggtgctgacg cacacggtct ccggcggcgt cacggtcaac
     1741 gactgcctgt tccatttcac gcagatcaat caaccgatgg gcggggtcgg cgcgtcgggc
     1801 tcgggcgcct atcatggcga atggggattc aagacgctga gcaagcttaa gccggtgttc
     1861 tatcgctcgg tgctcaaccg gctcgccgat ctctatccgc cttatggtgg gaggatcgcg
     1921 cggttggaga agatgctgcg gttcatgtcg taaccaccgt cgttccggga tgcacaaagc
     1981 gcagacccgg tatctcgaga ttccgggctc gatgctgcgt gtcgccccgg aacgatcaag
     2041 caaggatttg ggggatcgcg gtgactgaca cattcgattt cgtggtggtg ggcgcgggaa
     2101 gcggcggttg cgcagtggca agccggctct cggaggatgc ggccacttcg gtagcgctgc
     2161 tcgatgccgg cggcaaatgc gacaactggg tggtgaccac gccaggtgcg atcatcctga
     2221 tgatttcggg caagctcaac aactgggcgt tcaacaccgt gccgcagaag gggctgaacg
     2281 gccgcatcgg ctatcagccg cgcggcaagg gactcggcgg ctccagcgcc atcaacgcca
     2341 tggtctatat ccgcggccac cgcgtcgatt acgaccactg ggcttcgctc ggcaacaccg
     2401 gctggagtta cgccgacgtg cttccctgct tcaagcgctc ggaagacaat gccgagttcg
     2461 acggcgaatt tcacggcaag ggcggtccgc tccatgtctc gggcctgcgc tccgacaacc
     2521 cggtcaagga aatgtttctg cagggcgcgc gcgaggcgca gttccggatt cgcgacgatt
     2581 tcaacggcgc ggaacaggag ggacttggcg tctaccaggt cacacaaaac aacggcgagc
     2641 gctggagcgc ggcgcgcggt tacatccacc cgttcatggg cacgcgggga aacctgcgcg
     2701 tcgagacgca cgcgcaggcc acccgcatcc tgttcgaggg caagcgcgcg gtcggcgtcg
     2761 aatatctgca gggcaaacag aaaaagcagc ttcgcgcgcg tcgcgaggtc attctttccg
     2821 ccggcgcgtt tcaatcgccg cagctcttga tgctgtcggg cgtcggcgat gctgcttcgc
     2881 ttgccagata cggcatcgca agcctgcacc atctgccggg cgtcggacag aacctgcagg
     2941 accatcccga cttcgtgttc ggctttgcct ccgatgcccc ttatttctcg ggtctgtcct
     3001 ggagcggcat cggccgcatc ctcaaaggca tcgggcaata ccgccgcgag cggcgcgggc
     3061 ccttgacgtc gaacgtcgcc gaatgcggcg gctttctgaa gacgaggccg gacctcgacc
     3121 tgcccgatat ccagcttcat ttctgcatgg ccgtggtgga ggaccacggc cgcaagccgc
     3181 gctggggtac cggcttctcg tgccacgtct gcctgctccg cccgaagagc attggcagcg
     3241 tctggctcga cagcgccgat ccgctcgcgc cgcctgcgat cgatccgaac tttttgggcg
     3301 aggacagcga cctcgatacc atggttgcgg gcttcaagat gacgcggcgg ctgttggaag
     3361 cgccgacctt gcgcaaactg cagaaggccg acatgttcac gaacggcatt gcgagcgacg
     3421 aggatatccg caaggtactg cgtgcgaggg tcgataccgt ctatcacccg gtcggcacct
     3481 gcaagatggg cgtcaatgac gcaagcgccg tggtcgatcc cagactgaag gtctacggcc
     3541 tcgaaggcct gcgcgtggtc gatgcctcga tcatgccgac cttgatcggc ggcaacacca
     3601 atgcgccgac catcatgatc ggcgaaaaag ctgccgacat gatcagggcc gagatgcggg
     3661 cttgagcaag ctgttacgac aactcgtctt gcccggagga tgaattgtgg attgccgggt
     3721 caagccctgc aatgacgacg gaatagactg cccgacctgg tctatgaaag ccctcaatgc
     3781 cccatcggat ggtagccgcc ggcacccatc ggctgatagc cgccggggtt gcgggtgatg
     3841 gtggtggtat aggtggtgct ggtcgcggtg cgcgaaatct gcgtcgtgct tgcgctgtag
     3901 ccaggaccgg tcgtggtgac ggtattggtc tcgcggttga cggtggtggt gtaggactgc
     3961 gcctgggctg cagtcgctac aagtacaaat ccaaaccaaa ccagcactct catcatccga
     4021 ccctgttgtt gtcgcgagcg gcgttcacct agcaggggca ttttgacgaa gacttgtcgg
     4081 attagttcaa atgcgacgga ccagccggtc gatctcggtg cacaggcgtc gccgggctgg
     4141 tgctgagtct tgcctggagg atggattgcc gggccaagcc cggcaacgac cgcctctcct
     4201 gacgcaaagc ctcagggctt atcagcgcct gcgaggttgg ctaaggtagc catcaccgca
     4261 aaatcaaact gcgcgctttt agggagtgaa accgtggatc tgggtatcaa aggccgccgc
     4321 gccatcgtct gtgcatcgag caagggattg ggacgcgcct gcgcgattgc gctggccaat
     4381 gaaggcgtgc atgtcacgct gaccgcgcgc ggcgcggaag cgctgaaggc gaccgccgag
     4441 gagatccgca agaagagccc gggcgtcacc gtgacggaag tggtcgggga catcacgacg
     4501 cctgccggtc gcgaggccgt gctgaaagcc tgtcccgagc cggacattct ggtcaacaac
     4561 gccggtggcc cgccgcccgg cgacttccgc aactggaccc gcgacgactg gatcaaggcg
     4621 atcgatgcca acatgctgac cccgatcgag ttgatcaagg ccacggtgga cggcatgatg
     4681 gcgcgaaaat tcggccgcat cgtcaatatc acctcggccg cggtgaaggc gccgatcgag
     4741 atattggggt tgtccaacgg ggcgcgcgcc ggcctcaccg gcttcatcgc aggcatttcg
     4801 cgcaagacgg tcatcaacaa cgtgaccatc aacggcctgc tgccgggacc gtttgcaacc
     4861 gaccggctgc gcggcacggc gaaagcggaa gccgacaagc gcggtattac gccggaccag
     4921 ttgcttgccg agcgcgccaa gcagaacccc gccggccgct tcggcgatcc cgaggagttc
     4981 ggactgtttt gcgccttcct gtgcggcgca aaggccggct tcatcaccgg acagaacatc
     5041 ctgctcgacg gtggcgcctt cccgggtacg ctctagtgcg ttagcgccaa taccgagccg
     5101 gtgcatccgt ggtcgcattg gttggacggc tggaccgaac ctgttctact ttaccggcgg
     5161 tggagcttgg gagcaggtca acagaaactt cctgctcagc accgatacgg cggcgggtgt
     5221 cttcagtgaa tccgctgcgg gcacctttac cacaacgaaa tcaggttggg tcgccggcct
     5281 cggctatgag cggatgatcg ctcaaaactg gatcgtgcgc gccgaatggc tgcactatgg
     5341 cttcagcggc cttgg
//
LOCUS       DATLJT010001792         7650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3904991,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001792 DATLJT010000000
VERSION     DATLJT010001792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3904991"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="lepB"
                     /locus_tag="VM910_40425"
     CDS             <1..571
                     /gene="lepB"
                     /locus_tag="VM910_40425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVI68790.1"
                     /translation="THYALPFSPPVFSGRIFASQPKRGDVAVFRLPWDDSIDYIKRVI
                     GLPGDRIQMIDGLLHINGVAVKRERAQDYIDSDVGQYPTRVRRWRETLPNGVSYETLD
                     RVENSLYDNTAPYLVPPGHFFMMGDNRDNSNDSRVSPASGGVGYVPFENIIGRAEIIF
                     FSIGDGAHPWQVWRWPWSVHWHRLFRIVR"
     gene            complement(730..1245)
                     /locus_tag="VM910_40430"
                     /pseudo
     CDS             complement(730..1245)
                     /locus_tag="VM910_40430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1281..2177)
                     /locus_tag="VM910_40435"
     CDS             complement(1281..2177)
                     /locus_tag="VM910_40435"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68791.1"
                     /translation="MARKPRRRARPCTAPTTQIVLLGTGTPRPDPTRSGPATGLVVNG
                     MPYLVDFGPGVVRRIAAAYDKGATAFGFAAAALQTVFLTHLHADHTAGYPDLILTPWI
                     LGRRQPLDVYGPKGLRSMTGHVLKAWEIDIEDRVTGIDRLPRRGCRVNPHEIAPGVIY
                     VDRNIRVTAFPVRHGDLAAFGFRFETADRVIVISGDTAPTPSLAEHCEGCDVLIHEAY
                     SERTYERVSPQWQRYRRTHHTSSKQLAALATRVRPGLLVLYHRSNAGGAARSPDAEKQ
                     LLWEMRRLYRGRIVAGHDLDIL"
     gene            complement(2182..3183)
                     /locus_tag="VM910_40440"
     CDS             complement(2182..3183)
                     /locus_tag="VM910_40440"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HVI68792.1"
                     /translation="MTPPAAWLAVRAAAAVLMSVGALLFANATFAQTYPSRPVKLVVP
                     FPGGGPLDFTARLLAEKLAAAMKQAFVVENRPGASGNLGTEAVAKVAPDGYTLLFVLE
                     TPLTVSPSLYPKLAFDPSRDFTPIATAARFSLTLVVHPSVPVNSLAEFVAYAKARKEQ
                     PLIYGSGGGRGDPGHLTMEYFRMRAGFEGVHVPYKGNSQVVMNLVGGQIHAGFLATPG
                     VLPHIQDGRLKALAISSAQRAPLAPDIPTVAESGYPGFEVGFYQVMLAPAGLPEPIRA
                     TLEQEMHHALQSPDLQARLRGNALEPVVRGGDETAILLKAAAERWRAVITSANIQLD"
     gene            3294..4238
                     /locus_tag="VM910_40445"
     CDS             3294..4238
                     /locus_tag="VM910_40445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVI68793.1"
                     /translation="MQWADRNGRRLKLRDLHILLAVVQRGSMAKAAAELAISQPAISK
                     AISDIEHTLGLRLLDRGRNGIEPTVYGRALVRRGMAIFDELKQGVEELEFLADPTRGK
                     LNIGSTESIAAGLLPAVIDRFSRQHSGVHLNLVQAVISTLHYRELRERSIDLLLGRIP
                     ANFAEDDLEADVLFNDQVVVVAGERSRWARARGLKLADLANAPWILPPADTLPGSLAV
                     DLFRAAGLDMPAAPITTLSIHLCCQLTATERFVTTLPTSILRFNGKNLPLKVLPIRLP
                     IQLRPVGVVTLKNRTPNPVARLFIECVRGIVKPLADRK"
     gene            4569..4778
                     /locus_tag="VM910_40450"
     CDS             4569..4778
                     /locus_tag="VM910_40450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68794.1"
                     /translation="MVVAKAYPEPDERGRGKKSAVAALFPMVAPRRLQEARAVIAYRD
                     QDLGLFSVRENFRAGVSVYRTKAIT"
     gene            complement(5377..5541)
                     /locus_tag="VM910_40455"
     CDS             complement(5377..5541)
                     /locus_tag="VM910_40455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68795.1"
                     /translation="MACPPPSPRQRIPGRPTDAGDRFKPRGLLPRLYSRLLIALIVTW
                     IVLVILHMWS"
     gene            5780..5980
                     /locus_tag="VM910_40460"
     CDS             5780..5980
                     /locus_tag="VM910_40460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68796.1"
                     /translation="MRQKSQLVLPCDPDYTAHREAQKRRQETREWLLQRERANLAPSI
                     VPDVPEGQPKFERRRRYSRSRH"
     gene            complement(6098..6535)
                     /locus_tag="VM910_40465"
     CDS             complement(6098..6535)
                     /locus_tag="VM910_40465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68797.1"
                     /translation="MANGERQMPGHVEAAYRGAVDNILFLKKQQWLATNYVLLLYLAV
                     FLISAHYFSRTDAARNWLGVITIVGFVVHWWMLHSFQRSIEMFRNRLVWVYRSYFSED
                     ERAGLDLRLEPRSYWYQPEAYIGLIAVSLVGAILTAVYLWSVR"
     gene            complement(7049..>7650)
                     /locus_tag="VM910_40470"
     CDS             complement(7049..>7650)
                     /locus_tag="VM910_40470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68798.1"
                     /translation="AGKWRIGVGTTLRAKTLGIFGYGRIGATVAGYGKAFGMNVLVWA
                     RERSLERARLDGYATARSKAEFFERCDVVSLHLRLVAATRHIVTAEDLARMRPTAVLV
                     NTSRAPLIEPGALVAALRAGRPGYAAVDVFEHEPMRDPNLPLLQFEQVVATPHLGYVT
                     REEYETQFTDIFDQILAFAAGRPINVVNPQVLAATSARG"
BASE COUNT         1504 a         2357 c         2379 g         1410 t
ORIGIN      
        1 tacgcattat gcgctgccat tctcgccgcc ggtgttctcc gggcgcattt tcgcatcgca
       61 acccaagcgc ggcgacgtcg cggtgttccg cctcccctgg gacgactcga tcgactacat
      121 caagcgcgtc attggtcttc ccggcgaccg cattcagatg atcgacggcc tgctccacat
      181 caacggggtc gcggtcaaac gcgagcgcgc gcaagactat atcgacagcg acgtcggcca
      241 atacccaact cgagtcaggc gctggcgcga gaccctgccg aatggggtca gctacgagac
      301 gctcgatcgc gtcgagaatt ccttgtacga caacacggcg ccctacctcg taccgcccgg
      361 gcatttcttc atgatgggcg acaaccgcga caattccaac gacagcagag tgtcgcctgc
      421 gagtggcggg gtcggctatg tgccgttcga gaacatcatc ggccgcgccg aaatcatctt
      481 cttctcgatc ggtgacggcg cgcacccttg gcaggtgtgg cgctggccgt ggtcggtgca
      541 ctggcaccgc ttgttcagga tcgttcgctg aggaaggatg gaaaaaagag tccgacgccg
      601 ggaacggctg cgaccgtcaa ggaaatcgtg agctatcttg cttcccgcgg caatccgagc
      661 ttcctcgatc ttgatgattg agggagcgct cctaacggtg gatgcgcaca caggcggcga
      721 ccgagtcgct tagggcgtgg actcattaac gcgcaaccac agcctgattg atgcaagttg
      781 gataaaggcg aggtagttcg ccgcgagttt gtcatagcgg gtggcgacac ggcgacattg
      841 cttgatcttg ttgaagaacc gttcgactag attacgagcg cgataaagat gcgggctaaa
      901 gcagattggc tccttccgat tccgtttcgg cggaatgttt gcccacgcgc cgtgttggct
      961 cacgagggct ctgatccaat ctgcatcaaa tccacgatcc ccgagcagca tagcccccga
     1021 tttgaggcta gacagcaggg tgaggacgag acggttgtcg tgcgcttcgc cggtggtcaa
     1081 ggcgagccgc aaaggcattc cgttagtatc gacgacagca tggatcttgc tcgtcagtcc
     1141 gcctcgcgac cggcccatca gttgttcttt gttgcctgca atgcaggccc cgtgctgatg
     1201 aactcggaca atggacgtgt cgatcatctg caccgccgca ttatgttcgg tgaaaatgac
     1261 ccaaacctga caacccgggg tcacaaaatg tcgaggtcat gtccggcaac aatccggccc
     1321 cgatagagcc ggcgcatttc ccacagcaat tgcttctctg catccggcga tctcgccgcg
     1381 ccgcccgcgt tcgagcggtg atagaggacg agcaagcctg gccggacgcg cgtcgcgagc
     1441 gccgcgagtt gcttcgacga cgtgtgatgg gtgcgccgat agcgctgcca ctgcggcgaa
     1501 acccgttcat aggttcgctc cgaataggcc tcgtggatca gcacatcgca gccctcgcag
     1561 tgctcggcga ggctcggcgt gggggccgtg tcgccggaaa taacgatcac cctgtcagct
     1621 gtctcgaacc ggaatccgaa cgcggccaaa tcgccgtgcc gtaccggaaa agcggtgacg
     1681 cggatgttgc gatcgacgta gatgacgccg ggcgcgatct catgcgggtt aacccggcag
     1741 ccgcgccgcg gcagccggtc gatgcccgtc acgcgatcct cgatgtcgat ctcccacgcc
     1801 ttgagcacgt gcccagtcat cgagcgcaga cccttcggcc catagacgtc gagcggctgc
     1861 cggcggccaa ggatccaggg cgtcaggatc aggtcggggt atccggccgt gtggtcggca
     1921 tgcagatgcg tgagaaaaac tgtctggagg gccgcggcgg cgaagccgaa tgcggttgcg
     1981 cccttgtcgt aggctgccgc aatacggcga acgacgcctg gaccgaagtc gaccagatag
     2041 ggcatgccgt tgaccaccag cccagttgcg gggccagacc gcgtaggatc gggacgcggc
     2101 gtcccggtcc ccagtaggac gatctgcgtc gtcggcgcgg tgcacggtcg cgcccggcgg
     2161 cgaggcttgc gggccatgcg atcagtcgag ctggatgttg gcgctcgtga tcacggcgcg
     2221 ccatcgctca gcggccgcct tgagcaggat ggcggtttcg tcgccgcctc gcacgaccgg
     2281 ctcaagcgcg ttgcctcgca gccgcgcctg cagatcgggt gattgcaacg catggtgcat
     2341 ttcctgctcg agcgtggcgc ggataggctc cggcaggccg gccggcgcca gcatcacctg
     2401 gtagaagccg acctcgaagc cggggtagcc gctctccgcg accgtcggga tgtccggcgc
     2461 gagcggtgcg cgctgcgcac tggagattgc aagcgctttc aggcgcccat cctgtatatg
     2521 tggcagcacg ccgggtgtcg cgagaaagcc cgcgtggatt tgtccgccga cgagattcat
     2581 cacgacctgc gaattgcctt tgtagggaac gtgcacgccc tcgaaccccg cccgcatgcg
     2641 gaaatattcc atcgtcaagt gccccgggtc gccgcgcccg ccgccactgc catagatcaa
     2701 cggctgctcc tttcgcgcct tggcataggc gacaaattcc gccagcgaat tcaccggtac
     2761 agagggatgg acaacgaggg taagcgagaa gcgcgccgcc gtggcgatcg gcgtgaagtc
     2821 acgcgacgga tcgaatgcga gcttcggata aagcgacggg ctaacagtca gcggcgtctc
     2881 aaggacgaaa aggagcgtat agccatcggg cgccaccttg gcgaccgctt cggttccgag
     2941 attaccggac gcgcctggcc ggttctccac cacaaacgcc tgcttcatcg cggccgctag
     3001 cttctcggcg agcaggcgtg ccgtgaagtc gagcggtccg ccgcctggaa acggtacgac
     3061 gagctttacg ggccgggatg gataggtttg cgcaaaagtc gcgtttgcga aaagaagcgc
     3121 gccgacgctc atcagcaccg cggcggcggc ccgcactgcg agccatgcag ccggtggggt
     3181 catcggttac tcccgggttc ggacaatgga gcttctcgat cttgcgggag gcagacataa
     3241 acgtccaatt ctattatatg aaggttacat aacctttcga atagggtgct acgatgcaat
     3301 gggccgatcg gaacgggcga cgcctcaagc tacgcgacct gcacattctg ctcgctgtgg
     3361 tgcagcgggg cagcatggcg aaggcggcag ccgagctcgc gatctcgcag cccgccatct
     3421 cgaaggcgat ctccgacata gagcacacgc tcggccttcg cctactcgat cggggccgca
     3481 acggcatcga gccgacggtc tatggccgcg cgctcgttcg gcgcggcatg gcgattttcg
     3541 acgagctcaa acagggcgtc gaagaactcg agttcctcgc cgatccgacg agaggtaagc
     3601 tgaacatcgg cagtaccgaa agcatcgcgg cagggctgtt gcctgccgtc atcgaccgct
     3661 tctcgcgaca gcattcgggc gttcacctca atttagttca ggccgtcatc agtacactgc
     3721 attatcgcga actgcgcgag cggagcatcg atctgctgct tggacggata cctgccaatt
     3781 ttgccgagga cgatctcgaa gcggatgttc tgttcaacga tcaggtcgta gtggtggccg
     3841 gcgagcggag ccgatgggcc cgcgctcgcg gattgaaact ggcggacctt gccaacgcgc
     3901 cctggattct gccgccggcc gataccttgc ccggctcgct cgcggtcgat ctcttccgcg
     3961 ccgcaggtct ggacatgccg gccgcgccga tcacgacgct ttcaattcac ctgtgctgcc
     4021 agctcacggc gaccgagcgc ttcgtcacga cgctgccgac ctcgatcttg cgcttcaacg
     4081 gcaaaaacct gccgctgaag gtactcccaa tccggcttcc cattcagctg cggccggttg
     4141 gtgtcgtgac gttgaagaac cgcacgccaa atccagtggc acggcttttc atcgaatgtg
     4201 tgcgcgggat cgtgaagccg ctcgcagata ggaagtagga tagccgcggc caaaggcacc
     4261 ggagcacgtc agctcgtggc acatcgcgtc aattcgcggc gacgcagcac ttcggtcgct
     4321 ttcggagcga agcggacatt gactgagccg cgcttacaga aagcggattt atgagtacgc
     4381 gccctagttc tccatgaatg gtttcagaag ttgccgccat tcgctctcag actgacaaat
     4441 cttctcttct aagagacatg caactggcgg accttggccc ggcaatggag cgaccgccgc
     4501 ggaaattgta gcgacagttt tttgtcgcta ccattttttc tcggccgtcc gacgtttgcg
     4561 cgctcgcgat ggtggtcgca aaggcgtacc cggaaccgga cgaaagaggg cgcgggaaaa
     4621 aaagcgcggt ggccgcgctt tttccaatgg tggcaccgcg gcgattgcaa gaggcccgcg
     4681 ccgtcatcgc gtaccgcgat caggatttag gactattttc cgtgcgggaa aatttccgcg
     4741 cgggcgtgag tgtatacaga acgaaggcaa tcacctgaag cagcgccaca ctccatggcc
     4801 cacgtaggcg gcgcgatggc ccgtaactct caggcaccag ttttgatttt gaccgcccca
     4861 tacgcatcgg ccgtacgcat tgcgatggta attgcgaccg cagccgccta gcgcgaatgc
     4921 cggtgcaacc gacgataagg tgacggcagc aatcgcgacg ccgagcagca cggtcctgag
     4981 cgacttcatg aatttcatgc atacctccta ttgaggagcg atcgtggtca atcgcgatcc
     5041 tgcttgcact tcaagcgcgc gttaccgaga ttgattacga tacggggatg atgcattcaa
     5101 tcctctcgct ctgggtacgg acattcttgg cttgatcgcg atcggacaac agaccggata
     5161 ccaaacgaaa cccagggtgg agcaaagtgg agcaaaatag gtcgactgct ccgctcgggg
     5221 gtggtggtgg gagcagggtg tcacctgagc gatctcgttt cggcaaacgg gcgctcgcgc
     5281 attctcaaaa gacgttgctc gccgcatctc gcggcggctg caccgatgcg caattcgagc
     5341 gcgcggcggc gctcgggctt ggggcggcgc gagacatcat gaccacatgt gcaggatcac
     5401 caacacgatc caagtcacga tgagcgcgat cagcagccgc gagtagagcc gcggcagcag
     5461 gccgcggggc ttgaaacggt cgccggcgtc cgtggggcgg ccggggatgc gctggcgagg
     5521 cgacggcggc gggcaggcca tggttggctc ttagcgcccg gtggggaagg gcgccaggtc
     5581 gctacgcgtc ggtgcgcact ctacgacgct catctcgaga tcgatgcggg ccggcggccg
     5641 cagcgcggcc gacgttctcg attacaaacg tgtcatgatc ttcaagttcc ggtgatccca
     5701 tcgcgctaag cgcgtcgcca tccatactta atggaacaga caacggcagg tcgtcgttgt
     5761 gtggcaaagg gatggacaca tgcgtcaaaa gtcccaattg gttctgccct gcgaccccga
     5821 ctacacggcg catcgggagg cccagaagcg ccggcaggaa acccgcgaat ggctgttgca
     5881 gcgcgagcga gccaaccttg cgccctccat cgtgccagac gtgccggagg gccaaccgaa
     5941 gttcgaacgt aggcgccgct attcgcgctc gcgccattga ttggagctaa ggccctgcgc
     6001 tttgggggca ccgttgcgct acaacaatgc cctcccgcag ccggatacgc gtcgcgttct
     6061 cctagcgagt cggctggtaa cgacgaaaat taaaaagtta gcggaccgac cacaaataca
     6121 ccgcggtcaa aatggctccg accaatgaaa cggcgatgag cccaatgtaa gcctcgggtt
     6181 gataccaata tgaccttggt tccagccgca gatccaatcc cgcgcgctcg tcttcgctga
     6241 aatagctcct ataaacccaa accaggcgat ttctaaacat ctcgatcgag cgttgaaatg
     6301 agtgcaacat ccaccagtgg acgacaaacc caacaattgt aattacgcca agccaatttc
     6361 gtgcagcatc agtccggctg aagtagtgag ccgagatcag gaaaacggcc agatatagaa
     6421 gtaggacata gttggtcgcc agccattgct gctttttcag aaaaaggatg ttgtccaccg
     6481 cacccctgta ggccgcctct acatgccccg gcatttgccg ctcaccgttt gccatcggac
     6541 cctccctcag ttcggcttgc ctaagatgac atcgttaggg agccggctca acccgtctca
     6601 acggaggggt cgtgctaaat tgcacaagct gcaatcctat gggtgcgccc tgtgaggaag
     6661 gggggcgtgc cgatttttcc gagtgcaata tgcaatgaca gacgtgcgta cctgccggct
     6721 cggtttggcg tgacggaatg gtcgccggcg gttagtgttt gcatagggag gttgcaagcg
     6781 aacgaggcgg gcatgtcatt gcggataaca tttgcgcttg ctgtcatcat tactctgacc
     6841 gctgtcgcga ccgcgtcggc tgcggccaag aaacaaagag tccctcgcag cggctcgcag
     6901 gttcaggccg tcgctccacg caacgccgcg cccttcaacc catacaatcc cgcgctcaca
     6961 ggcgccggca gccctggcta caatcaaagc ctcatgacct ggaggtaagt gccggccgcc
     7021 gtgcattcag gcgtgaaaca cgtcgcgctc acccgcgcgc gctcgtcgct gccagcacct
     7081 gcggattgac gacgttgatc ggccgtcccg ccgcaaaggc gaggatctgg tcgaaaatgt
     7141 cggtgaattg cgtctcgtat tcctcgcgcg tcacatagcc gaggtgcggc gtcgccacga
     7201 cctgctcgaa ctgcagcagc ggcaaattgg gatcgcgcat tggctcgtgc tcgaacacgt
     7261 cgaccgccgc gtagccgggc cggcccgcgc gcagcgccgc caccagcgcg ccgggctcga
     7321 tcagcggcgc gcggctggtg ttgaccagca cggcggtcgg ccgcatgcgc gcgagatcct
     7381 cggcggtgac gatgtggcgc gttgccgcaa cgagccgcag gtgcagcgaa acgacgtcgc
     7441 agcgctcgaa gaattccgcc ttgctgcgcg ccgtcgcgta gccgtccaag cgcgcccgct
     7501 cgagcgatcg ctcgcgcgcc cacaccagca cgttcatgcc gaacgccttg ccatagcccg
     7561 cgaccgtcgc gccgatgcgg ccgtagccga aaatgccgag ggtttttgcg cgcaacgtcg
     7621 tgccgacgcc gatccgccac ttgccggcct
//
LOCUS       DATLJT010001793         5865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2065089,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001793 DATLJT010000000
VERSION     DATLJT010001793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065089"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VM910_40475"
     CDS             <1..595
                     /locus_tag="VM910_40475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HVI68799.1"
                     /translation="KIGISTEHIDFPGTQTISTEAALKGWMALGENVAHRGLKKLVIV
                     TSHGGNSAAMTLVAQDLRAYHGLLAVTTSWSRLSAAEQFFPAEEVRHGIHGGAVETSI
                     MLARHRDQVRQEAIADFRPSSIAMEASYRRLSTQRPAPFAWQAQDLHPSGAIGNATLA
                     TAEKGERLVDQGARAFIELLEDVDQFDVTIFAPAAAP"
     gene            671..949
                     /locus_tag="VM910_40480"
     CDS             671..949
                     /locus_tag="VM910_40480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HVI68800.1"
                     /translation="MSHTIREKKKLLARVGRIRGQVEALERSLNEETDCARIMHMIAG
                     IRGSVSGLMAEVVEDHVRTHLVDPKKNPGALNADAAEQLIDVVHTYLR"
     gene            955..1965
                     /gene="dmeF"
                     /locus_tag="VM910_40485"
     CDS             955..1965
                     /gene="dmeF"
                     /locus_tag="VM910_40485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HVI68801.1"
                     /translation="MTDSFGAFQPTPHAHVFLGRDHQRAERRTWAVIILCTIMMLAEI
                     IGGALFGSLALIADGLHMSTHAGALLLAALAYTFARRYAYDRSFTFGTGKFGDLAGYS
                     SAIVLAMIALLIAYEAVERLISPVPISFREAIPIAFLGLAVNVASAWLLSGGRDHDHY
                     HNHAHVHDHHHEHDHEHAHDHHHEHDHAHGTHHRDNNMRAAVVHVMADAAVSVLVIVG
                     LLLARAFGWLWMDPLAGLIGAVVIANWSFGLLRDTGAILLDRTPDLGIAEKVRQAVES
                     EGDRVTDLHLWRLGPGHLGAIVSVATTKAREAAHYRQRLAGFADLSHVTVEVQHAPPA
                     ND"
     gene            complement(1982..2236)
                     /locus_tag="VM910_40490"
     CDS             complement(1982..2236)
                     /locus_tag="VM910_40490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HVI68802.1"
                     /translation="MPGHPDAAIAKRLKRANGHLETIIEMIEQGRPCVEIAQQLRAVE
                     GAIANAKKALIQDHIGRGLKATGAKGGAALKDFRAIAKYL"
     gene            3119..5140
                     /locus_tag="VM910_40495"
     CDS             3119..5140
                     /locus_tag="VM910_40495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVI68803.1"
                     /translation="MLRLRKTRTNDRCSRPCQLAATALGIALGGPALAADLPVKAPMA
                     VYDWTGFYVGGHFGYGGGSLGPGTNPLPEQGVLFPPGVTGLIGGYQVGYNRQFANRLV
                     LGVEADATFTSPEDQAALGRMPVTPFNTTADYVGTVRGRIGYAFGRWMPYVTGGFAWG
                     HTRAFINQTDTDSGDVISALGHYQTGWTAGLGIEFAVSGNWTAKLEYEYVDLSRQTYD
                     LSGFGLANTSVEPRVSLAKFGLNYQFGDMPLRPADSGKIALPESDVWNVHAQATFLPQ
                     GYTAFRALYSGPDSLPPGGQLQNTWTTTAFLGVRLWEGGEFYFDPELAQGFGLNGTLG
                     VGGFPNGEAQKAGAAFPKIRPQRYYVKQTFGLGGEQEDVADGPNQLQGKRDIDRVTLV
                     VGRFAIGDFFDNNAYAHDPRADFMNWALWESASYDFPADLPGYTRGVMVELNRKNWAV
                     RGAVVETPTQPNSDILTYKGLGTVVEFEGRYNLFEQPGKVRFGVFETQGNTGSYRQAL
                     TAEMADPTLDINTIMASIRHINPKYGSYANVEQQIVQDVGLFARASWNDGQNEILSFT
                     DIDRSLSAGLSIKGSFWGRANDTVGIGGIINGLSDAHRDFLAAGGLGLLIGDGRLNYR
                     PEQILEAYYAYQVTKNFTVTADYQFIANPSYNADRGPVSIFACRLHGEF"
     gene            5156..>5865
                     /locus_tag="VM910_40500"
     CDS             5156..>5865
                     /locus_tag="VM910_40500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVI68804.1"
                     /translation="MTLQDLRASTLGQALRPNVWDAVALILVIGAMVLLAYGGEQTTL
                     PLSALEVTPVSLDPRNLPFYALRTTMRMLLAIVCSTIFTFVYSAIAAKSRRAEMVLIP
                     LLDILQSVPILGFLTFTVVFFLNLFPGQVLGAELACVFAIFTSQAWNMTFSMYQSIRN
                     VPKDLEEASQSFHLSGWQRFWRLDVPFAMPGLIWNAMMSMSGGWFFVVASEAITVGNT
                     TVTLPGVGSYVALAIEKR"
BASE COUNT         1066 a         1883 c         1820 g         1096 t
ORIGIN      
        1 gaagatcggg atttccaccg agcatatcga ttttccgggc acgcagacga tctcgaccga
       61 agcggcgtta aagggatgga tggcgctcgg cgagaacgtc gcgcacagag gcctgaaaaa
      121 gctcgtcatc gtcaccagcc acggcggcaa cagcgcagcg atgacgctcg tggcgcagga
      181 cctgcgcgcc tatcacgggc ttctcgccgt caccacgtca tggtcgcgcc tgtccgcggc
      241 ggagcaattt tttccggcag aggaagtgcg tcacggcatc catggcggcg cggtcgaaac
      301 ctcgatcatg ctggcgcgcc acagggatca ggtacggcag gaggcgatcg ccgacttccg
      361 cccatcgagc atagcgatgg aagcgagcta tcgccggctt tcgacgcagc ggccggcgcc
      421 gttcgcctgg caggcgcaag acctgcaccc gagcggcgcg atcggcaacg cgacgcttgc
      481 caccgccgaa aaaggcgagc ggctggtcga tcagggcgcg cgtgccttca tcgaattgct
      541 cgaagacgtc gatcaattcg acgtgacaat attcgcgcca gccgcagcac cgtaagggca
      601 gcgccttttt gcaaggggct cctcaaaacg cacattgaca tagcataggg gggtacccta
      661 tgtatgttcc atgtcgcaca ccatccggga aaagaagaag ctgctggcgc gggtcggccg
      721 gatccgcggc caggtcgaag cgctcgagcg ctcgctcaat gaggagaccg actgcgcgcg
      781 catcatgcat atgatcgcgg gcattcgcgg cagcgtttcg gggctaatgg ccgaggttgt
      841 cgaggatcac gtccgcaccc atctcgtcga ccccaagaag aatccgggcg cgctcaacgc
      901 cgacgccgcc gaacagctca tcgatgtggt ccacacctat ctgaggtgaa ggtcatgacc
      961 gattcgtttg gggcatttca gcccaccccg cacgcgcacg tcttcctcgg cagggatcat
     1021 cagagggccg aacgccgcac ctgggccgtg atcatcctct gcactatcat gatgctcgcc
     1081 gaaatcatcg gtggcgcgct gttcggctcg ctcgcgctga tcgccgacgg actgcatatg
     1141 tcgacgcatg ccggcgccct gctgctggcc gcgctcgcct acactttcgc ccgcagatac
     1201 gcttatgacc gaagcttcac tttcggcacc ggcaagttcg gcgatctcgc aggatattcc
     1261 agcgcgatcg tgctggcgat gattgcgctt ctgatcgcct acgaggccgt cgagcgcttg
     1321 atcagcccgg tgccgatcag cttccgcgaa gctatcccga tcgccttcct ggggcttgcc
     1381 gtcaacgtcg cgagcgcctg gctcttgtcc ggcgggcgtg atcacgatca ttaccataat
     1441 cacgctcacg tacacgacca tcaccacgag cacgatcacg agcacgcgca cgatcatcac
     1501 catgagcacg atcacgcgca tgggacccat catcgcgaca acaacatgcg ggccgcggtg
     1561 gtccatgtga tggccgatgc cgcggtctcg gtgctcgtga tcgtcgggct cctgctggcg
     1621 cgcgcgtttg gctggctctg gatggacccg ctcgcgggac tgatcggtgc ggtcgtgatc
     1681 gcaaactggt cgttcggcct tttgcgcgat accggcgcga tcctgctcga ccggacaccg
     1741 gatttgggca tcgcggagaa ggtgcggcag gcggtcgaga gcgagggcga ccgcgtcacc
     1801 gatctgcatc tgtggcggct tggaccggga catctcggcg cgatcgtgtc ggtggctacc
     1861 acgaaggccc gcgaagccgc gcattaccgg cagcggctcg caggcttcgc cgacctgtcg
     1921 cacgtcacgg tggaggtcca gcacgcgcct ccggccaacg actgagggcg acagcgacgc
     1981 ctcacagata tttggcgatc gccctgaaat ccttcagcgc ggcgccgcct ttcgccccgg
     2041 tcgccttcag gccgcggccg atatggtctt gaatgagggc cttcttggca ttcgcgatcg
     2101 cgccctcgac cgcccggagc tgctgggcga tctccacaca ggggcggccc tgttcgatca
     2161 tctcgatgat ggtttcgagg tggccgttgg cgcgtttcag gcgttttgcg atcgcggcat
     2221 cagggtgacc cggcatcggc gtctcctatc ctccccggag gataggccag atcctcccgg
     2281 gaggatagcg ccccaggctt gacagcgctc cataccgagc ctatttgagc agcgcgcttg
     2341 ctgccggcga ctgccgaagc gggcgcgatt caaccctcac gaagagacca tgggcagtac
     2401 ccgcaaaggc agcgccaatc cgatcggagc cgcgttgccg caacacattg agatcgccgc
     2461 gacggcggcg gctgcgcaag cgctgatggt cgccttgggc tgacccattt cgctcaccgc
     2521 tgccgcaacc gattgcggcc aggaggtgag cgatgctcga gaaactcgcg aaattcccga
     2581 ttgctcgatc ccggcgcggc gcgccgcgcg tgatcgtgcc cgccaacgac aatcgcctgg
     2641 gcgctcgccg gaacgagctg cggcgaggtc ggccacagct tgcctgtcgc tggttcgtcg
     2701 acgaggcagg acggcttggt tcgcggtggg agactgacgc gcccgaggat cccgggccat
     2761 cccggcgcga ccggagcatc gcaccttttt tcacagaact gtcttcgatc cgtcttatca
     2821 gacagggcac gtcggacccg cacaggactc gatccagccc ggaccgcttc gcgcgatgcg
     2881 gatgaggggc gtaagcggca cggcaaacag cgtgattgtg ttgcaccggc gtcacgcgac
     2941 catgcgatct aacggacatt ccggtctgcc tccttccaat tgcaaatgac ttgcaataac
     3001 gctgggaaaa atcgtgcctg cgcattgcag ttcaaaaccc gtgggacggg cggatttttt
     3061 ctcggcgaga gccttcgcga atgcgatgtt gacgtggtga cctgaagaga ttggcgcgat
     3121 gttgaggctg aggaagactc gcaccaatga ccgctgcagc cgaccatgtc agttggcggc
     3181 gacggcgctc ggcatcgcgc ttggcggccc ggcgctggca gcggaccttc ccgtgaaagc
     3241 gccgatggcc gtctacgatt ggaccggatt ttatgtcggc ggccattttg gctacggcgg
     3301 cggcagcctc gggcccggta ccaatcccct tcccgaacag ggcgtgctgt tcccgcctgg
     3361 cgtcaccggc ctgatcggcg gctaccaggt gggctacaac cgtcaattcg caaaccgcct
     3421 cgtgctcggc gtcgaagccg atgccacctt tacaagcccg gaggatcaag ccgcactggg
     3481 acgaatgccg gttacgccct tcaacacgac ggccgactat gtcggtacgg tgcgtggccg
     3541 catcggatac gcgttcggac ggtggatgcc ttatgtcacc ggcggcttcg cctggggaca
     3601 cacacgcgcc ttcatcaacc agaccgacac agactccggc gacgtcattt ctgccctggg
     3661 tcactatcag accggctgga ccgccggact cggtatcgag tttgccgtga gtggcaattg
     3721 gaccgccaag ctcgaatacg agtatgtcga cctatcgcgg cagacctacg atctgagcgg
     3781 cttcggactg gccaatacaa gcgtggagcc tcgcgtcagt ctcgccaaat tcggcctgaa
     3841 ctatcaattc ggcgatatgc ctctgaggcc ggccgacagc ggcaaaatcg cgttgcccga
     3901 gtccgacgtc tggaacgtcc atgcgcaggc gacgtttctg ccgcagggtt atacggcttt
     3961 tcgcgcgctc tatagcggtc ccgacagcct gccccccggc ggacagcttc agaacacctg
     4021 gacgacgacg gcctttcttg gcgtcaggct ctgggagggc ggcgagtttt acttcgatcc
     4081 cgagcttgca cagggtttcg gcctgaacgg cacgctgggc gtcggcggat tccccaacgg
     4141 ggaggcgcaa aaggcaggcg ccgcgttccc gaaaattcgc ccgcagcgct actacgtcaa
     4201 gcagacgttc gggctcggag gcgaacagga agatgtcgcg gacggcccca atcaactcca
     4261 gggcaagcgc gatattgatc gcgtcaccct cgtggtcggc cgcttcgcga tcggagactt
     4321 tttcgacaac aatgcctatg cgcacgatcc gcgcgcggat ttcatgaatt gggcattgtg
     4381 ggagtcagcc tcctacgact tcccggccga cctgcccggt tatacgcggg gggtcatggt
     4441 cgaactcaac cgcaagaact gggcggtgcg tggcgcggtt gtggagacgc cgacccagcc
     4501 caacagcgac atcctgacct acaaggggct cgggaccgtc gtcgagttcg aaggccgcta
     4561 caaccttttc gagcagcccg gcaaggtcag gtttggcgtc ttcgagaccc agggcaacac
     4621 cggcagctac cgtcaggccc tgacggccga aatggcggat ccaacgctcg atatcaacac
     4681 gatcatggcg agcattcgac atatcaaccc gaaatacggc tcctacgcca atgtcgagca
     4741 gcagatcgtt caggacgtcg gcctgttcgc gcgcgcaagc tggaacgacg gacaaaacga
     4801 gatcctgtca ttcaccgata tcgaccgcag cctgtcggcc gggctgtcca tcaagggcag
     4861 tttctgggga cgggcgaacg acaccgtcgg tatcggcggc atcatcaacg gattgtcgga
     4921 cgcgcatcgc gacttcctcg ccgccggcgg cctcggcctt ctgatcggcg atggccgcct
     4981 caactatcgc ccggaacaaa ttctcgaggc ctattacgcc tatcaggtca ccaagaactt
     5041 caccgtgacg gccgattatc aattcatcgc aaatccctcc tacaatgcgg accgcggtcc
     5101 ggtttcgatc ttcgcgtgcc gcctgcacgg cgaattctag gcgggggctt ttgcaatgac
     5161 gcttcaagat cttcgcgcct ccacgctggg acaggcactg cggcccaacg tctgggatgc
     5221 cgtcgcgctg atactcgtga tcggcgccat ggtgctgctc gcctatggcg gcgaacagac
     5281 gaccctgccc ctctcggcgc tcgaggtcac cccggtgtcg ctcgatccgc gcaacctgcc
     5341 gttctacgcc ctgcggacca cgatgcgtat gctgctcgcc atcgtctgct cgaccatctt
     5401 caccttcgtt tattcggcga tcgcagccaa aagccgccgt gcggaaatgg tgctgatccc
     5461 gctgctcgat atcctgcagt cggtgccgat cctgggcttt ctgactttca ccgtcgtgtt
     5521 tttcctgaac ctgtttccgg gccaggtgct cggcgcggag ctcgcctgcg tgttcgcgat
     5581 cttcacgagc caggcctgga acatgacgtt cagcatgtac cagtcgatcc gcaacgtgcc
     5641 gaaggacctg gaggaggcgt cgcaaagctt tcatttgagc ggctggcagc ggttttggcg
     5701 gctggacgta ccgttcgcca tgcccggcct gatctggaac gcgatgatgt cgatgtcggg
     5761 cggctggttc ttcgtggtgg cgtcggaagc gatcaccgtc ggcaacacca ccgtgacctt
     5821 gccgggcgtc ggctcctatg tggcgctcgc gatcgagaag cgcga
//
LOCUS       DATLJT010001794         4434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2293279,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001794 DATLJT010000000
VERSION     DATLJT010001794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293279"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="VM910_40505"
     CDS             <1..155
                     /locus_tag="VM910_40505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI68805.1"
                     /translation="RHNTPRVGRILMARGRDATDVAIVTSFGPCTLASFRVITDEVTY
                     AGQAAT"
     gene            346..3687
                     /locus_tag="VM910_40510"
     CDS             346..3687
                     /locus_tag="VM910_40510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVI68806.1"
                     /translation="MSIQVALHHVTHYTYDRPINLGPQIVRLRPAPHCRTRILSYSLT
                     VTPVQHFVNWQQDPHGNWLARFVFPEKTTEFSVISDLRADIEVINPFDFFVEPYAEKW
                     PFKFPPEMREDLAAFIEPDPVTPRLKEFIASISREPRNTVNFLVEINQRLQRMIRYLI
                     RMEPGVQPPEETLASRAGSCRDTAWLLVQIMRHLGLPARFVSGYLIQLRPDLKPLEGP
                     AGAEIDFTDLHAWSEVYLPGAGWIGLDATSGLFCGEGHVPLAATPHFRSAAPITGTVE
                     LCEISFAYDVSVERLAEQPRVTAPFSLEAWSALDALGEAIDADLRQHDVRLTMGGEPT
                     FVSIDDYQSAEWNTTALGPIKRKCADELIRRLRNRFAPGGLLHYGQGKWYPGESNPRW
                     AFALYWRRDGVPIWHDDSLIAHEAADRKASSGDARVFAEGIATRLGFSTDYAQPVYED
                     PADRILKEGLIAENIDPSDPKIDDPVERARILKLFDGKLGEPAGFVLPLQHSTAAAGT
                     EWRSEIWSTRRGRLFLVPGDSPLGLRLPMQSLPSLDPVEYPHVLLADPFAERVPLRDP
                     ADALGKNYKASNAHNDEISAPLAPETPVLQRPGTVVTGQASSRVPVRTALTVEARDGH
                     LCVFMPPVETLNDYLDLVETIEATATELNLPIHVEGYAPAPDPRLNVLKVTPDPGVIE
                     VNVQPAASWREAVTITNGLYEDAHHSRLGTNKFMLDGRHTGTGGGNHVVFGGLSATDS
                     PFLRRPDLLKSLVLYMLRRPSLSYLFSGLFVGPTSQAPRIDEARQDLLYELEIAMDKI
                     PKPGGDDWMPPWLVDRLFHNILVDVTGNTHRAEICIDKLFSPDSTTNRLGLVELRSFE
                     MPPDARMSLAQQLLLRALIAWFWRMPLDGKPARWGTALHDRFMLEHFVWEDFLDVLDD
                     LRRADYAFDPVWFEAQRQFRFPIYGTVQRGGVTLELRQAIEPWHVLNEDVPGGPVRLV
                     DSSLERLQVKASGLNADRHAVTCNGRRVPLTATGRSGEYVAGVRFKAWNLATGLQPTV
                     GLNAPLIFDIVDAWNRRSLGGCVYHVVHPGGRNYETFPVNSYEAEARRRARFQHHGHT
                     GGVIDVPNLEHSLEYPMTLDLRRPN"
     gene            complement(3969..>4434)
                     /locus_tag="VM910_40515"
     CDS             complement(3969..>4434)
                     /locus_tag="VM910_40515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HVI68807.1"
                     /translation="KIWDTLAIAEYLHEINPGARLFPSDRGQRAHCRSICGEMHSGFS
                     NLRSALPMNIKARFVGFKAWAGARADIDRITSIWRECFDAYGGPYLFGQRACAADAMY
                     APVCSRFVTYDVALDEDAAGYSCTMMDYPYMKEWTAAAKLEPDELEELDVEF"
BASE COUNT          898 a         1350 c         1265 g          921 t
ORIGIN      
        1 cgcgccacaa tacgccgcgt gtcggacgca ttttgatggc acgcggacgc gatgccaccg
       61 acgtggcgat cgttacttct ttcggacctt gtacccttgc gagctttagg gtcatcactg
      121 acgaagtgac atatgcgggg caggctgcaa cgtaatgcag tgccccaacc acaacgtggc
      181 gccgccaccc gggatcgatt gagctgccgt tcgcagtgcg gcccgttggc tataaggcct
      241 cgatgacgca tagtccccgg acggggtgtc cacagagtcg cgccattgac ggcccggaaa
      301 ttgcatggaa attggcaata tacaaatccg ggtgcgagcg gcagcatgtc catccaggtc
      361 gcgcttcacc acgtcaccca ttacacctat gaccggccaa tcaacctcgg cccacagatc
      421 gtccggctgc ggccagcgcc gcactgccgg actcgtattc tgagctattc cctaacggtg
      481 acgccggtgc agcatttcgt gaattggcag caagaccccc acggtaattg gcttgcccgc
      541 ttcgtcttcc cggaaaaaac taccgaattt tccgtcatat ccgatcttcg agcagacatt
      601 gaagtaatta atccgtttga ttttttcgtc gagccctatg cagaaaagtg gccgttcaag
      661 tttcctcccg aaatgcgcga agaccttgcc gccttcatcg aaccagatcc cgtaacgccg
      721 cgtttgaagg agtttatcgc ctcgatttcg cgcgagccgc gcaataccgt gaattttctg
      781 gtcgaaatca accagcgcct ccagcgaatg atccgctacc taattcgcat ggagcccggc
      841 gtacagccgc ccgaggaaac gcttgcttca cgcgctggct cctgccgcga cacagcgtgg
      901 ctgttagtgc aaatcatgcg gcaccttgga ctaccggctc gtttcgtttc cggttatctc
      961 attcagctgc ggcctgatct gaagccgctc gagggacctg ccggcgccga gatcgatttt
     1021 accgatttac acgcatggtc cgaagtttac ctgccaggcg ccggatggat cgggttggat
     1081 gcgacctcag ggcttttctg cggcgagggt cacgtaccgc ttgcggcgac gccccacttc
     1141 cgctcggccg ctccgattac cggcacggta gaactgtgcg aaatatcttt tgcgtacgac
     1201 gtgagtgtgg agcgcctcgc cgaacagccg cgtgtgacgg cgccgttctc gctggaggct
     1261 tggagcgcgc tcgatgcgct cggcgaagca atcgacgccg atctgcggca gcatgatgtg
     1321 cgtctgacca tgggcggcga gccgaccttc gtctccatcg acgactatca atcggcggaa
     1381 tggaacacga ctgcactcgg gccaatcaag cggaagtgcg ccgacgaatt gatccggcgg
     1441 ttgcgcaatc gtttcgcgcc aggcggatta cttcattatg gtcagggcaa atggtacccc
     1501 ggcgaatcga atccgcgctg ggcgttcgca ctgtattggc gccgcgatgg cgtaccgatc
     1561 tggcacgatg acagtttaat tgcgcacgaa gccgctgatc gcaaagcctc atccggcgat
     1621 gcacgagtat ttgctgaggg aattgcaacc cgcttaggat tctcgaccga ttacgcacag
     1681 cccgtctacg aagatcctgc ggaccggatc ctaaaagagg gattgatcgc ggagaacatc
     1741 gatccgagcg atccaaagat cgacgatccg gtggaacgcg cgcgcatcct gaaattattt
     1801 gacggtaaac tcggcgagcc ggccggcttc gtgctgccgc tgcagcactc gacggccgcg
     1861 gctggaacgg aatggaggag cgagatctgg agcacacgcc gcggccggct gtttctggtg
     1921 cccggcgatt cacctctcgg cttgcggcta ccgatgcaat cgctgcccag tcttgacccc
     1981 gtcgaatatc cgcatgtctt actggccgac ccgtttgccg aacgtgtacc gttgcgcgat
     2041 cccgccgatg cgttaggaaa gaactataag gcgagcaacg cgcacaacga cgagatatcg
     2101 gcgccgctcg cacccgaaac accagtgttg cagcgccccg gtacagttgt caccggtcaa
     2161 gcgtcatcca gagtgccggt gcgtacggct ctgaccgtag aagcgcgtga tgggcatttg
     2221 tgcgtgttca tgccgcctgt cgaaacgctc aatgattatc tcgacctagt cgagacgatc
     2281 gaggcgaccg cgaccgaact caacttgcct atccacgtcg aagggtacgc gccagcgccc
     2341 gatccacggc tcaatgtgct caaagtcacg cccgatcccg gtgttattga ggtcaacgtt
     2401 caaccggcag cgtcatggcg cgaggccgtc actatcacca atggccttta cgaggacgcg
     2461 caccactcac ggctcggcac caataaattc atgctcgacg gcagacacac cgggacgggc
     2521 ggtggcaatc acgttgtatt tggcggcttg agcgcaaccg acagtccatt cctccggcgt
     2581 ccggatctgc tcaagagctt ggtgctctac atgctgcggc ggccttccct gtcatatcta
     2641 ttttccggat tgttcgtcgg gccgaccagc caagctcccc gcatcgacga ggcgcgccaa
     2701 gatctactct acgaactcga gatcgcgatg gacaaaatac ccaaaccggg tggcgacgat
     2761 tggatgccac cttggttggt cgatcgcctg ttccacaaca tcctggtcga cgtcaccggc
     2821 aatacccatc gcgctgagat ctgcatcgac aaactgttct ccccggacag tacgaccaac
     2881 cgcctaggac tggttgagct gcgttctttc gaaatgccgc cggacgcacg catgagtcta
     2941 gcgcagcagt tgttgctgcg tgcgctcatc gcctggttct ggcgaatgcc tctcgatggc
     3001 aagccggcgc gttggggcac cgcgcttcac gaccgcttca tgctcgagca tttcgtctgg
     3061 gaagatttcc tcgacgtact ggatgatctc agacgcgccg attacgcctt cgatcctgtc
     3121 tggttcgagg cgcaacggca attccgtttc ccaatctacg gcaccgttca gcgtggaggc
     3181 gtcacgctgg agttgcgcca ggcgatcgag ccctggcatg tactaaacga ggatgtgccc
     3241 ggcggccctg tgcgtcttgt tgattcctcc ctcgagcggt tgcaggtcaa ggcatcgggg
     3301 ctgaacgccg accgtcacgc cgtaacttgc aacggtcgcc gcgtgccgtt gaccgcgact
     3361 ggccgctctg gcgaatatgt tgccggcgtg cggttcaagg cttggaatct ggcgacaggt
     3421 ctgcaaccga ctgtcggcct caatgcacca ctgatcttcg acatcgtgga cgcctggaac
     3481 cgccgttcgc tcggcggctg cgtgtatcac gttgtccatc cgggcgggcg taactacgag
     3541 actttcccag tcaattccta cgaggcagaa gcccgccgcc gcgctcgctt ccagcaccac
     3601 ggccacactg gcggggtcat cgacgtacca aacctcgaac actcgctgga atatccgatg
     3661 accctcgatc tgagacgtcc gaactagtca ctttccgctg cttgcttgcg tcaatttcga
     3721 ggaaggccta gtcggttgat cgattaagcc ggtaatgtcc ggttatggca caaagccggg
     3781 atggccgcgc ggcgtctaat atccgctatt gggggtaaag cggacattgc tcagacacgc
     3841 cgtgatgtct gctaaagatt tctcgacaaa atagtcgctg aaagccgttg gtcccaaatt
     3901 aattgaccca ggtcgcaaga cgtgcaccct taaatctatc tgatggcgga agctcttgat
     3961 ggaaaatatc aaaactcgac gtctagttct tcgagctcat ccggttcaag cttggccgcc
     4021 gcggtccatt ctttcatgta gggatagtcc atcatcgtgc aggaatagcc cgccgcgtcc
     4081 tcgtcgagcg caacgtcgta ggtcacgaag cgagaacaga ctggcgcgta catggcgtcc
     4141 gcggcgcagg cgcgctggcc gaacaggtaa ggtccgccat aagcgtcaaa acattcccgc
     4201 cagattgacg tgatgcggtc gatgtcagcg cgcgcgcccg cccaggcttt gaagccaaca
     4261 aaccgcgctt tgatgttcat tggcaaggcg gaacgcagat tgctgaaacc cgaatgcatt
     4321 tcgccgcaga tggagcggca atgagcgcgc tgcccccggt cggaaggaaa cagccgcgca
     4381 ccgggattga tctcgtgcag atattcggcg atcgccaagg tgtcccagat tttc
//
LOCUS       DATLJT010001795         4876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_3675382,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001795 DATLJT010000000
VERSION     DATLJT010001795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3675382"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /locus_tag="VM910_40520"
     CDS             <1..1620
                     /locus_tag="VM910_40520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68808.1"
                     /translation="EATLAPSLFETELEAESAPPPHAAMPPEPKPELPNPAAAQAVFM
                     AAPRDFHAVEPTAEPELPPPLAISEILLPHAAASASPRPALDPELPPDHPLEPGTRPT
                     GRVASPSERIAASEDAISEIPATPRPPVSSASFIAAARRAAQAAAAQPLGDNKGVPAK
                     AGSKDKGKGGEPSSMTSKIRSLLVAASVVVIVLGAFKMAMTLLDTGAPAPASESVSEP
                     QASSPAESPAKPAAPDQLTPSMTSPTPIGRQSLNNALPNSTEPMASVDIPQVAAPTIS
                     TTSAEDVTGAISTESDRAVIVPVPPSERLPDLIGGPALRAAALKGDASAAYEVGNRFA
                     EGKGVPQNYDEAVKWYDRAARAGLMPAVFRLGTFYEKGLGVKRDTDIARRYYLQAAER
                     GNAKAMHNLAVLDADGGGKGANYKSAAQWFRKAADRGVADSQFNLGILYARGIGVEQN
                     LAESFKWFSLAAAQGDADAALKRDDVAKRLDAQSLAAAKLAIQTFVAEPQPDDAVTVR
                     TPVGGWDSAAPQPTAAKPAAKPAAKKTAAAR"
     gene            1725..2513
                     /locus_tag="VM910_40525"
     CDS             1725..2513
                     /locus_tag="VM910_40525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVI68809.1"
                     /translation="MSADDRKDKWSGQAGPWATPPSRAPIAQPPRPRPALNRSRYWSA
                     TIFALLMLSVGIRAYKDLSQPEAWAYWKDQYFAPSLSASLIANADFDGSGRGHQALAI
                     RGTIGAAAASWLRERLDEAHLAPGDTVLLSSPGGDVEQAVIMGDIIRSRGLATAVGMV
                     STSGRVRPAYCASACVLVYAGGKPRYGVEGSLLGVHRFVTTTPVDDPVAETQRTAGMV
                     LGYFTRMGVSSAVVEAMSKTSDVRWLDTREALAMNLITNRVAKP"
     gene            2715..3632
                     /locus_tag="VM910_40530"
     CDS             2715..3632
                     /locus_tag="VM910_40530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVI68810.1"
                     /translation="MQIYLPIADLPVNIFLILAMGAAVGFVSGMFGIGGGFLMTPLLI
                     FIGIAPAVAVASVASHIAASSFSGAISYWRRRAIDPALALVLLIGGVGGTALGVSTFT
                     YLRSLGQLDLMIALSYVVLLTTVGAAMLYEGFRAILRTRRGATPPRRAHNWIHALPLK
                     IRFKRSKIYLSVIPVVIVGIVIGFIGAIMGIGGGFILVPVMIYLLRVPTSTVIGTSMV
                     LTLVTMLFATVLHAVTNHLVDAVLALILMVGGVTGAQFGARAGQKIRGEQLRLLLGLL
                     ILAVGIRFAIELVIRPDELFTIRETGVTQ"
     gene            3629..4411
                     /locus_tag="VM910_40535"
     CDS             3629..4411
                     /locus_tag="VM910_40535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HVI68811.1"
                     /translation="MRACALPAAAILVAASLAAAPARGERLIASVSNHRVTVTPNYSG
                     EELVLFGSVEKDASVPAARSNYDLVVTVLGPRADMVTRRKARVFGIWINSDYRQFLKV
                     PTYLALFANRPFDEIGTPEVQRRQQLGLNNVLLTQRVGPDYADVVPNDAFRSAFVRLQ
                     RQQGLYREDTSAVTFLTPTLFRTGIPLPAAVPIGTYDIEIKLFSNGALLTRTETAFEI
                     VKVGFEQFVASTARQNGLAYGLVTAVMALMTGWIASIVFRKD"
     gene            complement(4502..>4876)
                     /locus_tag="VM910_40540"
     CDS             complement(4502..>4876)
                     /locus_tag="VM910_40540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVI68812.1"
                     /translation="EPLSGDVIGFVVASKKPGSRPIYVSGDTTWFDGVAEVARRFNCG
                     VVLPFAGAAQTRGPFHLTMDTNDTIETARAFSDAVIVPVHTDGWAHFRQNAKDLRLTF
                     DALGFGKRLQLLEPGVRTAIEA"
BASE COUNT          825 a         1661 c         1537 g          853 t
ORIGIN      
        1 gaagctacgc ttgcgccatc ccttttcgag accgaactgg aagcagagtc cgcgccgccg
       61 ccacacgcgg cgatgccgcc ggagcccaag cccgaattgc ccaatcccgc cgcggcgcaa
      121 gccgtcttta tggcagcgcc gcgcgacttc cacgccgtcg agccgacagc cgagcccgaa
      181 ctgccgccgc cgcttgccat cagtgaaatt ctcttgccgc atgcggcggc gtcggcatcg
      241 ccgcgccccg cgctcgaccc cgagctgccg cccgatcatc cgctcgagcc gggcacgcgg
      301 ccgacgggcc gcgtcgcgtc gccctcggag cggatcgccg cgtccgagga cgcaatcagc
      361 gagattcccg ccacgcctcg tccgccggtc tcctccgcga gcttcattgc cgccgcgcgg
      421 cgcgccgcgc aggctgccgc cgcccaaccg ctcggcgaca acaagggtgt gcctgcgaag
      481 gcaggctcca aagacaaggg caagggcggc gagccgtcct ccatgacttc gaaaatccgc
      541 tcgctgctgg tcgctgcgag cgtggtcgtt atcgtgctcg gcgccttcaa gatggcgatg
      601 acgctgctcg ataccggcgc gccggcacct gcaagcgaaa gcgtgagcga gccgcaggca
      661 tcctcaccgg ccgagagccc ggccaaaccg gcggctcccg atcagctcac cccgtcgatg
      721 acctcgccga cgccgatcgg ccggcagtcg ctgaacaacg ccctgccgaa ttcgaccgag
      781 ccgatggcct ccgtcgacat cccgcaagtc gcggcgccta ccatttcgac cacttcggct
      841 gaagacgtca cgggtgcgat atcgaccgag agcgataggg ccgtgattgt accggtcccg
      901 ccgagcgagc gcttgcccga tctgatcggc ggtccggcgt tgcgcgcagc cgccctgaag
      961 ggcgatgcaa gcgcagccta tgaggtcggc aatcgcttcg ccgaaggcaa gggcgtgccg
     1021 caaaattacg acgaagccgt gaaatggtat gaccgcgcag cgcgcgccgg cctcatgccc
     1081 gcggtcttcc ggctcgggac cttctacgag aaaggcctcg gcgtgaagag ggataccgac
     1141 atcgcgcggc gctactatct gcaggctgcc gagcgcggca acgcgaaagc aatgcacaat
     1201 ctcgcggtgc tcgatgccga cggcggcggc aagggcgcaa actacaagag tgcggcgcag
     1261 tggttccgca aggcggccga ccgcggcgtc gccgacagcc agttcaatct cggcatcctc
     1321 tatgcgcgag gcatcggcgt cgaacagaac ctcgcggagt ccttcaaatg gttcagcctc
     1381 gccgcggccc agggcgatgc agacgcagcc ctcaagcgcg acgacgtcgc caaacgtctc
     1441 gacgcgcagt cgctggccgc agccaagctc gcaatccaaa ccttcgtcgc cgagccgcag
     1501 cccgacgatg ccgtcactgt cagaacgccg gtcggcggat gggactcggc tgccccgcag
     1561 ccgaccgcag ccaagccggc tgcgaagccc gccgccaaaa agaccgccgc agcgcgttaa
     1621 tttgatcgat ccgtgatttg ccgccgacat cggcggcagc tacctcaatg agggggcggt
     1681 gccgagcggc cgtgccggag agccccgccg tcgagaggat cgcagtgtct gccgatgatc
     1741 gcaaggacaa gtggagcgga caggccggac cgtgggcaac gccgccgtcg cgcgcgccga
     1801 tcgcgcagcc gccgaggccg cggcccgccc tcaacaggag ccgctactgg tcggctacca
     1861 tcttcgcgct gttgatgctt agcgtcggca tccgcgccta caaggacctg tcgcagccgg
     1921 aggcgtgggc ctactggaag gaccaatatt tcgcgccgag cctgagcgcg tcgctgatcg
     1981 ccaacgccga tttcgatgga tcgggccgag gccatcaggc actcgccatc cgcggcacga
     2041 tcggcgcggc cgcggcgagc tggctgcgtg agcggctcga cgaagcgcat ctcgcgccgg
     2101 gcgacaccgt gttgctttcc tcgccgggcg gcgatgtcga gcaggccgtc atcatgggag
     2161 acatcatcag atcacgcgga ctcgcaaccg ccgtcggcat ggtcagtacc tcgggccgtg
     2221 tccggccggc ctattgcgcg agcgcctgcg tgctggtcta cgccggcggc aagccgcgtt
     2281 acggcgtgga aggctccctg cttggggtcc atcgctttgt cacgaccacg ccggtggacg
     2341 atcccgtcgc cgagacgcag cgcacggccg gaatggtgtt gggttatttc accaggatgg
     2401 gcgtctcctc ggccgtcgtg gaggccatgt cgaaaaccag cgacgtgcgc tggctcgaca
     2461 ccagggaagc cttggcgatg aacctgatca ccaatcgcgt cgcaaagcct tgaggaccgt
     2521 ggcgggtccg cattaatctg aattcttcac cacacccgaa aaagtgcgcc ggagcgccgg
     2581 caaaaaagtc gcccctggcg aattctccag tccctccctg cggtgatttc ggctatgcag
     2641 aggcgcggca tcgaacccgc atcatgatgc gaatttctta atcgcgatcc tgaccgccaa
     2701 gcgggaaaac gcgcgtgcag atttatcttc cgattgccga cctcccggtc aacatcttcc
     2761 tcatcctggc gatgggtgcg gcggtcggct tcgtatccgg catgttcgga atcggcggcg
     2821 gatttttgat gacgccgctt ttgatcttca tcgggattgc acccgcggtc gcggtcgctt
     2881 ccgtcgcgag ccatatcgcg gcctcctcgt tctccggcgc gatttcctac tggcggcggc
     2941 gcgcgatcga tccggcgctc gcgctcgtgc ttctgatcgg cggtgtcggc ggaactgcgc
     3001 ttggcgtttc cactttcacc tatctgcgct cgctcggcca gctcgatctg atgatcgcgc
     3061 tgtcctacgt cgtcctgctc accaccgtcg gcgccgcgat gctctatgag ggctttcgcg
     3121 cgatcctgcg gacgcggcgt ggcgccacac cgccacgccg ggcccacaac tggatccatg
     3181 cgctgccgct gaaaatccgc ttcaaacgct ccaagatcta tctgtcggtg atcccggtcg
     3241 tgatcgtcgg catcgtcatc ggcttcatcg gcgccatcat gggcatcggc ggcggcttca
     3301 tcctcgtgcc agtcatgatc tatctcttgc gcgtgccgac ctcgaccgtc atcggcacct
     3361 cgatggtcct gacgctggtc accatgttgt tcgcaaccgt gttgcacgcg gttaccaacc
     3421 atctggtcga tgcggtgctg gcgttgatcc tgatggtcgg cggggtcacg ggcgcgcagt
     3481 tcggcgcccg cgccggacag aagattcgcg gcgaacagtt gcgtctgctg ctcgggttgt
     3541 tgattctcgc ggtcggcatc cgcttcgcga tcgagctcgt gatccgcccg gacgagctgt
     3601 tcaccatcag ggaaaccggg gtgacgcaat gagagcctgc gcactgcccg ccgctgcgat
     3661 tctggtggcc gcgagccttg cggccgcgcc ggcgcgtggc gaacggctga tcgcctcggt
     3721 ctccaaccat cgcgtcacgg tgacgccgaa ctattccggc gaggagctcg tgctgttcgg
     3781 ctcggtcgag aaggatgcct cggtaccggc cgcgcgcagc aattacgatc tggtcgtcac
     3841 cgtccttggc ccgcgcgccg atatggtcac ccgtcgcaag gcgcgcgtgt tcggcatctg
     3901 gatcaacagc gactaccggc aattcctgaa agtgccaacc tatctcgcgc tgttcgccaa
     3961 ccgtccgttc gacgagatcg gtacacccga ggttcagcgc cgacagcagc ttggcttgaa
     4021 caatgtgctg ttgacccagc gtgtcggtcc ggattacgcc gatgtcgttc ccaacgatgc
     4081 gttccgcagc gcttttgtgc ggctgcaacg ccagcagggc ctttaccgcg aggacacctc
     4141 cgcggtgacg ttcctgacgc cgacgctgtt tcgtaccggc attcccctgc ccgccgcagt
     4201 gccgatcggc acttacgaca tcgagatcaa gctgttctcg aacggcgcgc tgctgacgcg
     4261 caccgaaacc gcattcgaga tcgtcaaggt cggcttcgag caattcgtgg cctccaccgc
     4321 gcggcagaac gggctcgctt atgggctcgt caccgcggtc atggcgctga tgaccggctg
     4381 gatagcgtcg atcgtgttcc gcaaggatta actaaatcgt cattgcaagg agcgtgcgct
     4441 ccgaagcaat cccgagccgg gcttcggttt cctgaatcgc ttcgctcgca acgacggatc
     4501 gtcaagcctc gatcgccgtc cgcacgcccg gctccagcaa ttgcagccgc ttgccaaaac
     4561 cgagcgcgtc gaaggtgagg cgaaggtctt tcgcgttctg gcggaaatgc gcccagccgt
     4621 cggtgtgaac gggaacgatc accgcatcgg aaaatgcccg cgcggtctcg atcgtgtcgt
     4681 tggtatccat ggtgagatgg aaaggaccgc gggtttgcgc ggcgcccgca aagggcagca
     4741 cgacgccgca gttgaaacgc cttgccactt cggccacgcc atcgaaccag gtggtgtcgc
     4801 cgctgacata gatcggccgg ctgcccggct tcttcgatgc gacgacaaag ccgatgacgt
     4861 cgccggaaag cggctc
//
LOCUS       DATLJT010001796         2291 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2756346,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001796 DATLJT010000000
VERSION     DATLJT010001796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756346"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VM910_40545"
     CDS             complement(<1..456)
                     /locus_tag="VM910_40545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVI68813.1"
                     /translation="MMRSCRPRTAALAAVAFTFASHAALAQKKYDTGANDTEIKIGNI
                     MPYSGPASAYGVIGKTEEAYFNKINAEGGINGRKIKFISYDDAYSPPKAVEQARKLVE
                     SDEVLVIFNPLGTPSNTAIEKYLNTKRVPQLFVATGASRWNDPKNFPWTM"
     gene            complement(463..1479)
                     /locus_tag="VM910_40550"
     CDS             complement(463..1479)
                     /locus_tag="VM910_40550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI68814.1"
                     /translation="MSAVDDVVTEGQAVEAVPKRAMTLGAGTSLTVLALLLVIPFFAK
                     NFIIFQMTMLLIYGLAVLALNILTGGSGQFSLGQSAFYAIGAYTSAILMEQYDVNYAL
                     TLPVAGVLCFAAGFLFGQPALRLSGVYLALATFALATAMPQLLKLGFFEKWTGGVQGL
                     VVTKPDPPFHLPISQDMWLYYFTLVITIAIYIFSVNLLRSRSGRAFMAIRDNEIAASA
                     MGVDVALYKTLAFGVSAGITGVAGGLGAIAVQFVAPDSYTITLAIALFLGMVVGGVGW
                     LPGSIVGSAFIIFVPNIAESISKGLSGAVFGVLLFLVIFLVPHGARQVAIVSQQMIGK
                     FRKT"
     gene            complement(1482..>2291)
                     /locus_tag="VM910_40555"
     CDS             complement(1482..>2291)
                     /locus_tag="VM910_40555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HVI68815.1"
                     /translation="AVVMIYQAIDHLNFAQGEMAMFSTFVSWQLMQWGIPYWWAFLLT
                     LAFSFVAGIAIERILFKPLAKAPILTQVAGFIALFSIINSCAGLIWDFTIKQYPTPFG
                     SAPFLGSQLISTHQAGMIGVTVLLLIALYIFFQYTRVGLAMRAAASLPESARLVGINT
                     SWMIALGWGMAAGIGAIAGILIAPVVFLEPNMMGGVLIYGFAAAVLGGLTSPFGAVIG
                     GFLVGIVENLAGTYIPGVGNELKLPIALALIITVLVVKPAGLFGRPIVKRV"
BASE COUNT          487 a          676 c          748 g          380 t
ORIGIN      
        1 catggtccag ggaaaattct tcggatcatt ccatctggag gcgccggtgg cgacgaagag
       61 ctgcggcacc ctcttggtgt tgagatattt ctcgatcgcc gtgttcgagg gcgtgccgag
      121 cgggttgaag atcacgagga cctcgtcgct ctccacgagc ttgcgcgcct gctcgacggc
      181 cttgggcggc gaatacgcat cgtcatagga gatgaatttg atcttgcggc cattgatgcc
      241 gccttcggcg ttgatcttgt tgaagtacgc ctcttcggtc ttgccgatca cgccgtaggc
      301 cgaggccggc ccgctatagg gcatgatgtt gccgatcttg atctcggtat cgttggcgcc
      361 ggtatcgtat tttttttgcg caagggcggc gtgggacgca aaggtgaagg ccaccgccgc
      421 aagcgcggcg gtgcgtggtc ggcaggaacg catcatatct cctcaggtct tcctgaattt
      481 accgatcatc tgctggctga cgatggcgac ttgcctggcg ccgtgcggca ccaggaaaat
      541 gacgagaaac aggagcacgc cgaacacggc gccggagaga cccttggaga tgctttccgc
      601 gatgttgggc acgaagatga tgaaggccga acccacgatc gatccgggca accagccgac
      661 gccgccgacc accatgccga ggaacagagc gatcgccagc gtaatggtgt agctgtcggg
      721 cgcgacgaac tgcaccgcga tcgcacccag cccgccggca acgccggtga tccctgcgga
      781 aacgccgaac gcgagcgtct tgtacagcgc gacgtcgacg cccatcgccg atgccgcgat
      841 ctcgttgtcg cggatcgcca tgaaggcgcg accggaacgc gagcgcagga ggttcacgga
      901 aaagatgtag atcgcgatcg tgatcaccag cgtgaagtaa tagagccaca tgtcctgcga
      961 gatcggcagg tggaacggcg gatccggctt ggtcacgaca aggccctgca cgccgccggt
     1021 ccatttctcg aagaagccga gtttcaggag ctgcggcatc gccgttgcca gcgcaaacgt
     1081 cgcgagcgcg agatagacgc cggacagccg cagcgccggc tggccgaaca ggaagccggc
     1141 agcaaagcac agcacgcccg cgaccggcag cgtcagcgcg taattgacgt cgtactgctc
     1201 catcaggatc gccgaggtgt aggcgccgat cgcgtagaac gcgctctggc cgagcgagaa
     1261 ctggccgctt ccgccggtca ggatgttgag cgcgagcacc gcgagcccgt agatcaggag
     1321 catcgtcatc tggaagatga tgaagttctt ggcaaagaac ggtatgacga gcagcagcgc
     1381 cagcaccgtc agcgaggtgc ctgcgcccag cgtcatggcc cgcttcggaa cggcctctac
     1441 ggcctggcct tctgtgacga cgtcatcaac agcgctcatg ctcagactcg cttcacaatg
     1501 ggccggccga acaggccggc gggtttgacg accaacacgg tgatgatcag cgccagtgcg
     1561 atcggcagct tcagctcatt gccgacgccg gggatatagg tgccggcaag attctcgacg
     1621 atgccgacca gaaaaccgcc gatcacggcg ccgaacggac ttgtcagtcc gccgagcacc
     1681 gcggcggcaa agccgtagat cagcacaccg cccatcatgt tcggctcgag aaacaccacg
     1741 ggcgctatca ggattccggc gatcgccccg atgccggccg ccatgcccca gccaagcgcg
     1801 atcatccaag aggtgttgat gccgacgagc cgcgccgatt ccggcaggga ggcggcggcg
     1861 cgcatggcaa gtccgacccg cgtatattgg aagaagatat agagcgcgat cagaagcagc
     1921 acggtaacgc cgatcatgcc ggcctggtgg gtcgagatga gctggctgcc gaggaagggg
     1981 gccgagccga acggcgtcgg atattgcttg atggtgaaat cccagatcag gccggcgcag
     2041 gaattgatga tggagaacag cgcgatgaag cccgcgacct gggtcaggat cggcgctttc
     2101 gcgagtggct tgaagaggat gcgctcgatc gcaatgcccg cgacgaaaga gaaggcgagg
     2161 gtgagaagga aagcccacca gtaggggatg ccccattgca tcaattgcca ggagacgaag
     2221 gtcgagaaca tcgccatttc gccctgcgcg aaattgagat ggtcgatcgc ctggtagatc
     2281 atgaccaccg c
//
LOCUS       DATLJT010001797         1775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_914332,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001797 DATLJT010000000
VERSION     DATLJT010001797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914332"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VM910_40560"
     CDS             <1..1311
                     /locus_tag="VM910_40560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HVI68816.1"
                     /translation="ALLKSAFGLTEAPVNIYSMAGMTWALSSHYFPLAYLVLGPALRA
                     LDVRMEEAALMAGGSYRQVFAKVTLPLLRPSILSALLLLFVMGMASYEVPRLIGRPAR
                     IDVFTTDIQGAIIAAPPAFGVASALGLTLLAVCMLAVFFYRRATRHAESFATITGKGY
                     RPTRVKLGRARWPVALGVAAMFILALGLPLITLVWQSFFRNLAQPFFSRGAQATLDNY
                     EFILSYPIFLAALKTSVLLAAMAATIVAGLTFVMAWIAQRSLPRYGFILDALAFAPIA
                     IPGVIVGAGILVAYLMLPIPIYNTIWILLVAYVTLYLPYGMRFASSGISQIHRELEEV
                     AAVSGAGLAQIFLRILIPLLAPVLLAGWIYVFVLAVRELGASIFLVGPGTHVLGTISL
                     TMWEEGGSYGAVAALGVIQIVPLVVIVAGLRSIELRIQRRAQRS"
     gene            complement(1392..>1775)
                     /locus_tag="VM910_40565"
     CDS             complement(1392..>1775)
                     /locus_tag="VM910_40565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVI68817.1"
                     /translation="LAWVPFLMQRLDHEFMIRPSEAPLLKKKPSDYMRDMYYSSQPIE
                     IQDRQAMECTFRMINAETQLLYSSDYPHWDFDLPSTIYDLPFLSEKAKHNILGGTAAR
                     LFKLPPRNEKQKENLQRFGNLAAAA"
BASE COUNT          258 a          575 c          570 g          372 t
ORIGIN      
        1 gcgctgctca aatcggcgtt cgggctcacc gaggcgcccg tgaacatcta ttcgatggcg
       61 gggatgacct gggcgttgtc gagccactat tttccgctcg cctatctggt gctggggccg
      121 gcactgcgag cgctcgacgt gcgcatggaa gaggccgccc tgatggccgg cggaagttac
      181 cggcaggtct tcgccaaggt cacgttaccg ttgttgcggc cctcaatcct ttcggcgctg
      241 ctgctcctgt tcgtgatggg catggcctcg tatgaggtgc cgcgcctcat cggtcggccg
      301 gcgcgcatcg acgtgttcac caccgacatc cagggcgcca tcatcgcggc gccgccggcc
      361 ttcggagtcg ctagcgcgct cggattgacg ctgctcgcag tctgcatgct cgccgtattc
      421 ttctatcgcc gcgccacccg gcacgcggaa tcgtttgcca ccatcaccgg caagggctat
      481 cggcccaccc gcgtcaagct cggacgcgcc cgctggccgg tcgcgctcgg cgtcgccgcc
      541 atgtttatcc tcgcactcgg actgccgctg atcacgctcg tctggcagtc gttcttccgc
      601 aatctggcgc agccgttctt ctcccgcggc gcgcaggcga cgctcgacaa ttacgagttc
      661 atcctgagtt atccgatctt tctcgcggcg ttgaaaacga gcgtgctgct ggcggcgatg
      721 gccgcgacga tcgtcgccgg cctcaccttc gtcatggcct ggatcgcgca gcgatccctg
      781 ccgcgctatg gcttcatcct cgatgcgctc gccttcgctc cgatcgcgat cccgggcgtg
      841 atcgtcggcg cgggtatcct ggtcgcctat cttatgctgc cgatcccgat ctacaacacg
      901 atctggatcc tgctggtcgc ctacgtgacc ttgtatcttc cttacggcat gcggttcgcg
      961 tcgagcggga tcagccagat ccatcgcgag ctcgaggagg tggcggcggt ttcgggcgcc
     1021 gggctcgccc agatcttctt gcgcatcctc attccgctcc tggcgccggt gctgctggca
     1081 ggctggatct atgttttcgt gctggcggtg cgcgagctcg gcgcgtcgat cttcctcgtc
     1141 ggtcccggca cccatgtgct cggaaccatc agtctgacca tgtgggagga gggcggcagc
     1201 tacggcgcgg tcgcggcgct gggtgtgatc cagatcgttc cgctcgtcgt catcgttgcc
     1261 ggcctgcgat cgatcgagct gcgcatccaa cgccgcgcgc aacggtcgta gcgtgggaaa
     1321 agccgcaagc gcaaaaacaa aaccgcctcc cggctggagc cgggaggcgg ccgatggcga
     1381 ggtcggcggc ttcaggccgc ggcggcgaga ttgccgaacc gctggaggtt ttctttctgc
     1441 ttctcgttgc gcggcggcag cttgaacaag cgcgccgcgg tgccgccgag gatgttgtgc
     1501 ttggccttct ccgacaggaa gggcagatcg tagatcgtgg acggcaggtc gaaatcccag
     1561 tgcggataat ccgacgagta gaggagctgc gtctccgcgt tgatcatgcg gaacgtgcac
     1621 tccatcgcct gccgatcctg aatctcgatg ggctgtgagg agtagtacat gtcgcgcata
     1681 tagtccgatg gcttcttctt cagcagcggc gcctccgagg gccggatcat gaattcgtgg
     1741 tcgagccgct gcatcaggaa tggaacccac gcgag
//
LOCUS       DATLJT010001798         1815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2294505,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001798 DATLJT010000000
VERSION     DATLJT010001798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294505"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VM910_40570"
     CDS             <1..754
                     /locus_tag="VM910_40570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HVI68818.1"
                     /translation="RPVRLMPGVPSPLANRKPGDIDFWVVRENTEGEYSSVGGRMFPD
                     TEREFVTQQTVMTRIGVDRILKFAFELAASRPKKHLTSATKSNGISITMPYWDERVEA
                     MAKNYSKVKWDKYHIDILTANFVLHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPS
                     GNINPERDFPSVFEPVHGSAPDIAGQGIANPIGMIWSGAMMLEHLGEKEAGEAILAAI
                     ERTLGERTLRTRDLGGNADTTACGKAVAEMVE"
     gene            complement(823..1737)
                     /locus_tag="VM910_40575"
     CDS             complement(823..1737)
                     /locus_tag="VM910_40575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVI68819.1"
                     /translation="MRGVFVDANDSLAEIFERQRRDGDPDVRVHQDPNIEPEQWPAIL
                     GGAEIGVVDHTALPTAIAKQCTGLKHVVFLGTGARSYMNPDELAELGVSVHLIKGYGD
                     TAVAESAIALMWASARVLAQMDREMRAGNWLREDAMQLTGKTLGLVGFGGIAAEVARI
                     AKGAGMRVIAWNRSPKQHPGVEFVPLDRLLADSHVVSIHLLLNDETRGFITREKIAAM
                     RPGVILVNTARGAIVDEAAMIEALRSGHIRHAGLDVFNIEPLPKDHPLTKIPNVTLSA
                     HSAFRTPEASENLIHAAWEHCRRVARGG"
BASE COUNT          327 a          584 c          575 g          329 t
ORIGIN      
        1 tcgcccggtt cggctgatgc cgggtgtgcc gtcgccgctt gccaaccgca agcctggcga
       61 tatcgatttc tgggtggtgc gggaaaacac cgaaggcgaa tattcatccg tgggcgggcg
      121 aatgtttccg gataccgagc gcgagttcgt cacgcagcag acggtgatga cccggatcgg
      181 ggtcgaccgt atcctgaagt tcgctttcga gcttgccgcg tcgcggccga agaagcacct
      241 gacctcggcg accaagtcga acggcatctc catcaccatg ccctactggg acgagcgcgt
      301 ggaagcgatg gcgaagaatt attcgaaggt gaagtgggac aagtaccaca tcgacatcct
      361 gaccgcgaat ttcgtgctgc atccggactg gttcgatgtc gtggtcggct ccaacctgtt
      421 cggcgacatt ctgtccgatc tcggtcccgc ctgcaccggc acgatcggca ttgcgccgtc
      481 cggcaacatc aatccggaac gcgatttccc gagcgtgttc gagccggtgc acgggtctgc
      541 gcctgatatc gcggggcagg ggatcgcaaa cccgatcggc atgatctggt caggcgcgat
      601 gatgctggag catctcggcg agaaggaagc gggcgaagcg atcctcgccg cgatcgagcg
      661 cacgctcggc gaacggacgc tgcgcacccg cgatctcggc ggcaatgccg acacgacggc
      721 ctgcggcaag gcggtcgcgg aaatggtaga gtaggaccgg caccggttat cattgtcgtc
      781 ccggctcgcg cttcgcttgc ctgggacgac gagatcgaaa tttcagccgc cccgcgccac
      841 cctgcggcaa tgctcccacg ccgcgtgaat cagattctcg ctcgcttccg gcgtgcggaa
      901 cgccgaatgc gccgacagcg tgacgttggg aattttcgtt aatggatgat ccttcggcag
      961 cggctcgatg ttgaacacgt cgagcccggc atggcggata tggcccgacc tcaacgcctc
     1021 gatcatcgca gcttcgtcga cgatcgcgcc gcgcgcggtg ttgaccagga tgacgcccgg
     1081 ccgcatcgcg gcgatttttt cgcgcgtgat gaagccgcgc gtctcgtcgt tcaacagaag
     1141 atggatcgag acgacatggc tgtcggcgag cagcctgtcg agcgggacaa actcgacgcc
     1201 cggatgttgc ttcggcgacc tattccaggc gatcacacgc atgccggcgc ccttcgcaat
     1261 gcgcgccacc tccgcggcga tgccgccgaa gccgaccagg ccgagcgtct tgccggtgag
     1321 ctgcatcgca tcttcgcgca gccagttgcc ggcgcgcatc tcgcggtcca tctgcgccag
     1381 cacgcgcgcg gaggcccaca tcagcgcaat cgccgattcc gcgaccgccg tgtcgccata
     1441 acccttgatc aaatgcacgg aaacgccgag ttcggcgagc tcgtccggat tcatgtagct
     1501 gcgcgcaccg gtgccgagaa agacgacgtg cttcagcccc gtgcattgct ttgcgatcgc
     1561 ggtcggcagc gcggtgtgat cgacgacgcc gatctcggcg ccgcccagga tggccggcca
     1621 ctgctcgggc tcgatattgg ggtcctggtg cacgcgcaca tcgggatcgc cgtccctgcg
     1681 ctgccgctcg aaaatctccg cgagactgtc attggcgtca acaaagactc cgcgcaccgc
     1741 aacctccctt tgttcggtta ttcaggacgc aacgccggca agtgcaagca cggtgtgcat
     1801 caggacattg gcgcc
//
LOCUS       DATLJT010001799         3838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U13703 k141_2067235,
            whole genome shotgun sequence.
ACCESSION   DATLJT010001799 DATLJT010000000
VERSION     DATLJT010001799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37939581
            Sequence Read Archive: SRR8487025
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 08:47:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,118
            CDSs (total)                      :: 8,073
            Genes (coding)                    :: 7,854
            CDSs (with protein)               :: 7,854
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 41
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 80 of 219
            Pseudo Genes (incomplete)         :: 156 of 219
            Pseudo Genes (internal stop)      :: 20 of 219
            Pseudo Genes (multiple problems)  :: 34 of 219
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2067235"
                     /isolate="SMAG_U13703"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="38.535556 N 121.772778 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1516)
                     /locus_tag="VM910_40580"
     CDS             complement(140..1516)
                     /locus_tag="VM910_40580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HVI68820.1"
                     /translation="MRRIDHAEAASEFIAAKAHQEFHDRRLWDLRVKRDREAHAIPEW
                     EELRNLASQIKEHTLSRLADYLEQFESRARENGAHVHWARDAVEHNQILYDILSARQA
                     KTLVKSKSMLTEECDMRPFLERRDIEVIETDLGERIQQLDDEPPSHIVVPAVHKLRGD
                     VSAVFTRTIGAEPGNDDVHYLAETQRRTTRPYFLRAEAGMTGANFAVAETGSFVVCTN
                     EGNADLSANVPKLHIASIGIEKIIPKTEHLGVFIRLLSRSALGSPITQYTSHFRAPRT
                     GGELHIVLVDNGRSERLGMRDFWYSLKCIRCGACMNTCPVYRRSGGLSYGATYSGPIG
                     VIIDPTFNIRKYSNLPFASTLNGSCTNVCPVKINIHEQIYKWRQIIAERNQLPFVKKE
                     MMRVAGKVLADPRLYRAAISAAGLAIEHLPRFAIYNWLNPWGRQREIPEAPGQTFRSW
                     YIERRSRP"
     gene            complement(1513..2256)
                     /locus_tag="VM910_40585"
     CDS             complement(1513..2256)
                     /locus_tag="VM910_40585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HVI68821.1"
                     /translation="MRVGLFIPCYIDAFFPAVGIATLELLERLGVDVVYPLNQTCCGQ
                     LMANSGSQKDSAGAEALFVENFKDFDHIVTPSGSCAYHVREHLDAIPQSTQAQKVRSN
                     TYELVEFLHDVLKVEAFPWAEFPHKVGLHNSCGTLRKLRTAKASEIDEPFFSKPLDLL
                     RKVRGIEFVTPSRPDECCGFGGTFSIFEEPVSAKMGYDKVADHHAAGAEYIVSADMSC
                     LMHQKGCAERVGLEIRFIHIAQILNGDRG"
     gene            complement(2359..>3838)
                     /locus_tag="VM910_40590"
     CDS             complement(2359..>3838)
                     /locus_tag="VM910_40590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HVI68822.1"
                     /translation="AVFGAAFGLMPIGWIILNVIFLYQLTLERGYFKVMQDSIAGVSQ
                     DRRLQLLLIAFCFGAFFEGASGFGTPVAVTGAILIGLGFSPLAASGLSLIANTAPVAY
                     GALGTPIIALAAVTGLDLYDLSGMVGRQLPFFSLIVPFWLIWAFAGWRGMLEIWPATL
                     VAGVSFAIPQFVISNFHGPWLVDVLASVVSMACLIAFLNYWRPQKLWTTTTPRGQQGE
                     AAGSAVSAEPASTVGVPVAPAHAEVIKAWIPWLILSIAVILWGTPQFKALLDNLSIVR
                     IPVTGLHNLVERVPPVVLRPTPEAAVYLFNWLSATGSGILVAAVVAGLVMGFSPLGLV
                     RTYWNTLKLVRYSLLTIACMLAIGFITRYSGIDATLGLAFANTGPLYPLFGTMLGWLG
                     VALTGSDTSSNVLFGSLQRITAEQLGLNPVLMSAANSSGGVMGKMIDAQSIVVASTAT
                     RWYGHEGDILRYVFLHSLALAVLVGILVSLQAYVWPFSALVR"
BASE COUNT          807 a         1123 c         1197 g          711 t
ORIGIN      
        1 ccgcccattc gcagaagagc ttcccggaat tcttcgagca gcggccggga actcatttcg
       61 aacagcggta tctccggaag cgggggaacc ggcgtgggct ggctcgcgcg cacctctgcg
      121 agaatcgtgt cgcggctcgt cacggtcggc tcctgcgctc gatataccag ctgcgaaagg
      181 tttgccccgg cgcttctgga atctcgcgct ggcgacccca cggattgagc caattgtaga
      241 tggcgaagcg cggcagatgc tcgatcgcaa ggccagccgc cgagatggca gcgcgataca
      301 gcctcgggtc ggccagcacc ttgccggcga cgcgcatcat ttctttcttc acgaacggaa
      361 gctggttgcg ctcggcaatg atctgccgcc atttgtagat ctgctcgtga atgttgatct
      421 tgaccgggca aacgttggta caactgccgt tgagagtgga ggcaaacggg aggttgctat
      481 atttgcggat attgaaagtg ggatcgatga tgacgccgat cggacccgag taagtcgcgc
      541 cataactcag ccctccgctt cggcggtaga ccgggcaggt attcatgcac gctccacacc
      601 ggatgcattt cagcgaatac cagaaatcgc gcatgccgag acgctcggag cgtccgttgt
      661 cgacgagcac gatatgaagc tccccacccg tgcgcggcgc ccgaaagtgc gacgtgtatt
      721 gggtgatcgg cgagcccagg gcgctgcggg acaacaggcg gatgaatact ccgagatgct
      781 cggtctttgg gatgatcttt tcgattccga tcgacgcgat gtggagtttc ggtacgttgg
      841 cgctgaggtc ggcattgccc tcattggtac aaaccacaaa gctgccggtc tcagcgacag
      901 caaaatttgc gccggtcatg ccggcttcgg cccgcagaaa gtacggtcgc gtcgtccggc
      961 gctgcgtctc agccagataa tggacgtcat cgttgccggg ctccgccccg atcgttcgcg
     1021 taaataccgc ggagacgtcg ccgcggagct tgtggaccgc tggcacgacg atgtggctcg
     1081 gcggctcgtc gtcgagttgc tggatgcgct cgccgagatc ggtctctatc acctcgatgt
     1141 cgcgcctttc gaggaacgga cgcatgtcgc attcctctgt cagcattgac ttgctcttga
     1201 cgagcgtttt ggcctgccgc gccgagagaa tgtcgtagag gatctgatta tgctcgacgg
     1261 cgtcgcgggc ccagtgcacg tgcgcgccgt tctctctcgc ccggctttcg aattgttcga
     1321 gatagtccgc gagccgtgac agcgtgtgtt ccttgatttg cgaggcaaga ttgcgcagct
     1381 cctcccattc tggaattgcg tgcgcctcgc ggtcgcgctt gactcggaga tcccaaagcc
     1441 gccgatcatg gaattcctgg tgcgccttgg cggcgatgaa ttcggaggct gcctcggcat
     1501 gatcgatccg cctcatccgc ggtctccatt gaggatctgc gcaatatgga tgaagcggat
     1561 ctcgagacca acccgctcgg cgcaaccctt ttggtgcatg aggcacgaca tgtctgcgga
     1621 aacgatgtat tcggcgccgg cagcgtgatg atcggcaact ttgtcgtaac ccatcttggc
     1681 cgaaaccggc tcttcgaaaa tggaaaaagt gccgccgaag ccgcagcatt cgtccggtcg
     1741 gctcggggtc acgaattcga tgccgcgcac cttgcggagc agatcgagtg gcttggagaa
     1801 aaacggctcg tcgatctccg atgcttttgc ggtgcgaagc ttgcgaagcg tgccgcaaga
     1861 attgtgaagc ccgaccttgt gcggaaattc ggcccaaggg aaggcttcga ctttcagtac
     1921 atcatgcagg aattcgacca actcgtaggt attgctgcga accttctgcg cctgcgtgga
     1981 ctgtgggatg gcgtccaaat gttcccgcac gtggtaggcg cagcttcccg atggggtgac
     2041 gatatggtcg aaatccttga agttctcgac gaacagcgcc tccgccccag cggaatcctt
     2101 ttggctcccg ctgttcgcca tcagctggcc gcagcaggtc tggttgagcg gatagacaac
     2161 atcgaccccg agccgttcca gtagttccag tgtggcgatg ccgaccgccg gaaaaaaagc
     2221 atcgatgtaa cagggaataa acaatcccac tctcatgatc gatgctcttc aactcgatgt
     2281 cgcgtcagag cagcttgttc aagctggtac cgggccgaca ctctctattg ggccggatac
     2341 agcggcgcgc cgatcgcgtc agcgcactaa ggcgctgaag ggccagacat acgcctgaag
     2401 cgagacgaga atgccgacca gcacggccag agccagcgag tgcaagaaga cgtagcggag
     2461 gatgtcgcct tcatggccgt accagcgcgt cgcggtgcta gcgaccacga tgctctgggc
     2521 gtcgatcatt ttgcccatga caccgcccga gctgttcgct gcagacatca agactgggtt
     2581 gaggcccagc tgttcagccg tgatgcgttg caggctgccg aacagcacgt tcgacgaggt
     2641 atcggaaccg gtgagcgcca cgccgagcca gcctagcatc gtgccgaaca gcgggtagag
     2701 cgggcccgtg ttggcgaagg caagaccgag cgtcgcatcg ataccggaat agcgggtaat
     2761 gaatccgatt gcgagcatgc acgcgatcgt caaaagcgag tagcgtacaa gcttgagcgt
     2821 attccaatag gtgcgcacga ggcctaaggg cgaaaagccc atgaccaggc ccgcaacgac
     2881 ggcggccacc aggatgccgg agccagtggc ggacaaccaa ttgaatagat agacggcagc
     2941 ttcgggtgtt ggcctaagca cgacgggagg cacccgctcg accaaattgt gcaaacctgt
     3001 cactgggatg cgcacgatcg agaggttatc gagcaaagcc ttgaactgcg gcgtgcccca
     3061 taggatcacg gcgatgctga ggatgagcca gggaatccag gccttgatga cctcagcgtg
     3121 agccggcgca accgggacac cgacggtcga cgcaggctcc gccgatactg cgctccctgc
     3181 cgcctcgccc tgctgcccgc gcggcgttgt cgtcgtccaa agcttttgcg ggcgccaata
     3241 gttgaggaag gcgatcaagc aggccattga caccacggat gcgagaacgt cgacaagcca
     3301 ggggccgtga aaatttgaga tcacaaattg tgggatggca aagctgacgc cggccacgag
     3361 ggtggccggc cagatctcga gcatgccgcg ccagccggcg aaggcccaga tcaaccagaa
     3421 cggcactata agtgaaaaga acggaagttg ccggccgacc atcccgctga ggtcgtagag
     3481 atcgaggccg gtcaccgccg ccagcgcaat aatcggcgtg cccagcgcgc cgtaggcgac
     3541 tggcgcggta ttggcgatca acgacaatcc ggaagcagcc agcggtgaaa accccagtcc
     3601 gatcaggatc gcaccggtca cggccacggg cgttccgaaa cccgacgcac cctcgaagaa
     3661 ggcgccgaag cagaaggcga tcaagagcag ctgcagccga cggtcctggc tcacgcccgc
     3721 gatgctgtcc tgcatcacct tgaagtagcc ccgctccaac gtgagctggt agaggaagat
     3781 gacgttgaga atgatccagc cgatgggcat gaggccgaac gcagcaccaa acacagcg
//
